LOCUS       DAZKHF020000001        65512 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2201S31241
            SAMN43267800-rid25106413.denovo.001, whole genome shotgun sequence.
ACCESSION   DAZKHF020000001 DAZKHF020000000
VERSION     DAZKHF020000001.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267800
            Sequence Read Archive: SRR30307263
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 65512)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 65512)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 65512)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350581.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350581.2
            Genome Coverage       :: 151x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:21:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,652
            CDSs (total)                      :: 5,557
            Genes (coding)                    :: 5,320
            CDSs (with protein)               :: 5,320
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 237
            CDSs (without protein)            :: 237
            Pseudo Genes (ambiguous residues) :: 0 of 237
            Pseudo Genes (frameshifted)       :: 102 of 237
            Pseudo Genes (incomplete)         :: 130 of 237
            Pseudo Genes (internal stop)      :: 52 of 237
            Pseudo Genes (multiple problems)  :: 41 of 237
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..65512
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267800-rid25106413.denovo.001"
                     /serotype="O 26:H11"
                     /isolate="2201S31241"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-01"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            <1..503
                     /locus_tag="ACHOSC_RS00005"
                     /old_locus_tag="pgaptmp_000001"
     CDS             <1..503
                     /locus_tag="ACHOSC_RS00005"
                     /old_locus_tag="pgaptmp_000001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="glycine zipper 2TM domain-containing protein"
                     /protein_id="HGU4546019.1"
                     /translation="CLLPVAMLASFTLAGCQSNADDHAADVYQTDQLNTKQETKTVNI
                     ISILPAKVAVDNSQNKRNAQAFGALIGAVAGGVIGHNVGSGSNSGTTAGAVGGGAVGA
                     AVGSMVNDKTLVEGVSLTYKEGTKVYTSTQVGKECQFTTGLAVVITTTYNETRIQPNT
                     KCPEKS"
     gene            519..1058
                     /locus_tag="ACHOSC_RS00010"
                     /old_locus_tag="pgaptmp_000002"
     CDS             519..1058
                     /locus_tag="ACHOSC_RS00010"
                     /old_locus_tag="pgaptmp_000002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5384 family protein"
                     /protein_id="HGU4546020.1"
                     /translation="MKKVFLCAILASLSYPAIASSLQDQLSAVAEAEQQGKNEEQRQH
                     DEWVAERNREIQQEKQRRANAQAAANKRAATAAANKKARQDKLDAEASADKKRDQSYE
                     DELRSLEIQKQKLALAKEEARVKRENEFIDQELKHKAAQTDVVQSEADANRNMTEGGR
                     DLMKRVGKAEENKSDSWFN"
     gene            complement(1153..2730)
                     /gene="guaA"
                     /locus_tag="ACHOSC_RS00015"
                     /old_locus_tag="pgaptmp_000003"
     CDS             complement(1153..2730)
                     /gene="guaA"
                     /locus_tag="ACHOSC_RS00015"
                     /old_locus_tag="pgaptmp_000003"
                     /EC_number="6.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005130181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003922 - GMP synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016462 - pyrophosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006177 - GMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing GMP synthase"
                     /protein_id="HGU4546021.1"
                     /translation="MTENIHKHRILILDFGSQYTQLVARRVRELGVYCELWAWDVTEA
                     QIRDFNPSGIILSGGPESTTEENSPRAPQYVFEAGVPVFGVCYGMQTMAMQLGGHVEA
                     SNEREFGYAQVEVVNDSALVRGIEDALTADGKPLLDVWMSHGDKVTAIPSDFVTVAST
                     ESCPFAIMANEEKRFYGVQFHPEVTHTRQGMRMLERFVRDICQCEALWTPAKIIDDAV
                     ARIREQVGDDKVILGLSGGVDSSVTAMLLHRAIGKNLTCVFVDNGLLRLNEAEQVLDM
                     FGDHFGLNIVHVPAEDRFLSALAGENDPEAKRKIIGRVFVEVFDEEALKLEDVKWLAQ
                     GTIYPDVIESAASATGKAHVIKSHHNVGGLPKEMKMGLVEPLKELFKDEVRKIGLELG
                     LPYDMLYRHPFPGPGLGVRVLGEVKKEYCDLLRRADAIFIEELRKADLYDKVSQAFTV
                     FLPVRSVGVMGDGRKYDWVVSLRAVETIDFMTAHWAHLPYDFLGRVSNRIINEVNGIS
                     RVVYDISGKPPATIEWE"
     gene            complement(2799..4265)
                     /gene="guaB"
                     /locus_tag="ACHOSC_RS00020"
                     /old_locus_tag="pgaptmp_000004"
     CDS             complement(2799..4265)
                     /gene="guaB"
                     /locus_tag="ACHOSC_RS00020"
                     /old_locus_tag="pgaptmp_000004"
                     /EC_number="1.1.1.205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004151980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003938 - IMP dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IMP dehydrogenase"
                     /protein_id="HGU4546022.1"
                     /translation="MLRIAKEALTFDDVLLVPAHSTVLPNTADLSTQLTKTIRLNIPM
                     LSAAMDTVTEARLAIALAQEGGIGFIHKNMSIERQAEEVRRVKKHESGVVTDPQTVLP
                     TTTLREVKELTERNGFAGYPVVTEENELVGIITGRDVRFVTDLNQPVSVYMTPKERLV
                     TVREGEAREVVLAKMHEKRVEKALVVDDEFHLIGMITVKDFQKAERKPNACKDEQGRL
                     RVGAAVGAGAGNEERVDALVAAGVDVLLIDSSHGHSEGVLQRIRETRAKYPDLQIIGG
                     NVATAAGARALAEAGCSAVKVGIGPGSICTTRIVTGVGVPQITAVADAVEALEGTGIP
                     VIADGGIRFSGDIAKAIAAGASAVMVGSMLAGTEESPGEIELYQGRSYKSYRGMGSLG
                     AMSKGSSDRYFQSDNAADKLVPEGIEGRVAYKGRLKEIIHQQMGGLRSCMGLTGCGTI
                     DELRTKAEFVRISGAGIQESHVHDVTITKESPNYRLGS"
     gene            4427..5797
                     /gene="xseA"
                     /locus_tag="ACHOSC_RS00025"
                     /old_locus_tag="pgaptmp_000005"
     CDS             4427..5797
                     /gene="xseA"
                     /locus_tag="ACHOSC_RS00025"
                     /old_locus_tag="pgaptmp_000005"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009318 - exodeoxyribonuclease VII
                     complex [Evidence IEA];
                     GO_function: GO:0008855 - exodeoxyribonuclease VII
                     activity [Evidence IEA];
                     GO_process: GO:0006308 - DNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII large subunit"
                     /protein_id="HGU4546023.1"
                     /translation="MLPSQSPAIFTVSRLNQTVRLLLEHEMGQVWISGEISNFTQPAS
                     GHWYFTLKDDTAQVRCAMFRNSNRRVTFRPQHGQQVLVRANITLYEPRGDYQIIVESM
                     QPAGEGLLQQKYEQLKAKLQAEGLFDLQYKKPLPSPAHCVGVITSKTGAALHDILHVL
                     KRRDPSLPVIIYPTAVQGDDAPGQTVRAIELANQRNECDVLIVGRGGGSLEDLWSFND
                     ERVARAIFASRIPVVSAVGHETDVTIADFVADLRAPTPSAAAEVVSRNQQELLRQVQS
                     TRQRLEMAMDYYLANRTRRFTQIHHRLQQQHPQLRLARQQTMLERLQKRMSFALENQL
                     KRTGQQQQRLTQRLNQQNPQPKIHRAQTRIQQLEYRLAETLRVQLSATRERFGNAVTH
                     LEAVSPLSTLARGYSVTTATDGNVLKKVKQVKAGEMLTTRLEDGWIESEVKNIQPVKK
                     SRKKVH"
     gene            complement(5794..6009)
                     /locus_tag="ACHOSC_RS00030"
                     /old_locus_tag="pgaptmp_000006"
     CDS             complement(5794..6009)
                     /locus_tag="ACHOSC_RS00030"
                     /old_locus_tag="pgaptmp_000006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417005.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HGU4546024.1"
                     /translation="MELHCPQCQHVLDQDNDHARCPSCGEIIEMKALCPDCHQPLQVL
                     KACGAVDYFCQHGHGLISKKRVEFVLA"
     gene            complement(6078..7550)
                     /gene="der"
                     /locus_tag="ACHOSC_RS00035"
                     /old_locus_tag="pgaptmp_000007"
     CDS             complement(6078..7550)
                     /gene="der"
                     /locus_tag="ACHOSC_RS00035"
                     /old_locus_tag="pgaptmp_000007"
                     /EC_number="3.6.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417006.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase Der"
                     /protein_id="HGU4546025.1"
                     /translation="MVPVVALVGRPNVGKSTLFNRLTRTRDALVADFPGLTRDRKYGR
                     AEIEGREFICIDTGGIDGTEDGVETRMAEQSLLAIEEADVVLFMVDARAGLMPADEAI
                     AKHLRSREKPTFLVANKTDGLDPDQAVVDFYSLGLGEIYPIAASHGRGVLSLLEHVLL
                     PWMEDLAPQEEVDEDAEYWAQFEAEENGEEEEEDDFDPQSLPIKLAIVGRPNVGKSTL
                     TNRILGEERVVVYDMPGTTRDSIYIPMERDGREYVLIDTAGVRKRGKITDAVEKFSVI
                     KTLQAIEDANVVMLVIDAREGISDQDLSLLGFILNSGRSLVIVVNKWDGLSQEVKEQV
                     KETLDFRLGFIDFARVHFISALHGSGVGNLFESVREAYDSSTRRVGTSMLTRIMTMAV
                     EDHQPPLVRGRRVKLKYAHAGGYNPPIVVIHGNQVKDLPDSYKRYLMNYFRKSLDVMG
                     SPIRIQFKEGENPYANKRNTLTPTQMRKRKRLMKHIKKNK"
     gene            complement(7668..8846)
                     /gene="bamB"
                     /locus_tag="ACHOSC_RS00040"
                     /old_locus_tag="pgaptmp_000008"
     CDS             complement(7668..8846)
                     /gene="bamB"
                     /locus_tag="ACHOSC_RS00040"
                     /old_locus_tag="pgaptmp_000008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamB"
                     /protein_id="HGU4546026.1"
                     /translation="MQLRKLLLPGLLSVTLLSGCSLFNSEEDVVKMSPLPTVENQFTP
                     TTAWSTSVGSGIGNFYSNLHPALADNVVYAADRAGLVKALNADDGKEIWSVSLAEKDG
                     WFSKEPALLSGGLTVSGGHVYIGSEKAQVYALNTSDGTVAWQTKVAGEALSRPVVSDG
                     LVLIHTSNGQLQALNEADGAVKWTVNLDMPSLSLRGESAPATAFGAAVVGGDNGRVSA
                     VLMEQGQMIWQQRISQATGSTEIDRLSDVDTTPVVVNGVVFALAYNGNLTALDLRSGQ
                     IMWKRELGSVNDFIVDGNRIYLVDQNDRVMALTIDGGVTLWTQSDLLHRLLTSPVLYN
                     GNLVVGDSEGYLHWINVEDGRFVAQQKVDSSGFQTEPVAADGKLLIQAKDGTVYSITR
                     "
     gene            complement(8857..9477)
                     /gene="yfgM"
                     /locus_tag="ACHOSC_RS00045"
                     /old_locus_tag="pgaptmp_000009"
     CDS             complement(8857..9477)
                     /gene="yfgM"
                     /locus_tag="ACHOSC_RS00045"
                     /old_locus_tag="pgaptmp_000009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ancillary SecYEG translocon subunit YfgM"
                     /protein_id="HGU4546027.1"
                     /translation="MEIYENENDQVEAVKRFFAENGKALAVGVILGVGALIGWRYWNS
                     HQVDSARSASLAYQNAVTAVSEGKPDSIPAAEKFAAENKNTYGALASLELAQQFVDKN
                     ELEKAAAQLQQGLADTSDENLKAVINLRLARVQVQLKQADAALKTLDTIKGEGWAAIV
                     ADLRGEALLSKGDKQGARSAWEAGVKSDVTPALSEMMQMKINNLSI"
     gene            complement(9495..10769)
                     /gene="hisS"
                     /locus_tag="ACHOSC_RS00050"
                     /old_locus_tag="pgaptmp_000010"
     CDS             complement(9495..10769)
                     /gene="hisS"
                     /locus_tag="ACHOSC_RS00050"
                     /old_locus_tag="pgaptmp_000010"
                     /EC_number="6.1.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004821 - histidine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006427 - histidyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine--tRNA ligase"
                     /protein_id="HGU4546028.1"
                     /translation="MAKNIQAIRGMNDYLPGETAIWQRIEGTLKNVLGSYGYSEIRLP
                     IVEQTPLFKRAIGEVTDVVEKEMYTFEDRNGDSLTLRPEGTAGCVRAGIEHGLLYNQE
                     QRLWYIGPMFRHERPQKGRYRQFHQLGCEVFGLQGPDIDAELIMLTARWWRALGISEH
                     VTLELNSIGSLEARANYRDALVAFLEQHKEKLDEDCKRRMYTNPLRVLDSKNPEVQAL
                     LNDAPALGDYLDEESREHFAGLCKLLESAGIAYTVNQRLVRGLDYYNRTVFEWVTNSL
                     GSQGTVCAGGRYDGLVEQLGGRATPAVGFAMGLERLVLLVQAVNPEFKADPVVDIYLV
                     ASGADTQSAAMALAERLRDELPGVKLMTNHGGGNFKKQFARADKWGARVAVVLGESEV
                     ANGTAVVKDLRSGEQTAVAQDSVAAHLRTLLG"
     gene            complement(10880..11998)
                     /gene="ispG"
                     /locus_tag="ACHOSC_RS00055"
                     /old_locus_tag="pgaptmp_000011"
                     /gene_synonym="gcpE"
     CDS             complement(10880..11998)
                     /gene="ispG"
                     /locus_tag="ACHOSC_RS00055"
                     /old_locus_tag="pgaptmp_000011"
                     /gene_synonym="gcpE"
                     /EC_number="1.17.7.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046429 - 4-hydroxy-3-methylbut-2-en-1-yl
                     diphosphate synthase activity (ferredoxin) [Evidence IEA];
                     GO_process: GO:0044237 - cellular metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin-dependent
                     (E)-4-hydroxy-3-methylbut-2-enyl-diphosphate synthase"
                     /protein_id="HGU4546029.1"
                     /translation="MHNQAPIQRRKSTRIYVGNVPIGDGAPIAVQSMTNTRTTDVEAT
                     VNQIKALERVGADIVRVSVPTMDAAEAFKLIKQQVNVPLVADIHFDYRIALKVAEYGV
                     DCLRINPGNIGNEERIRMVVDCARDKNIPIRIGVNAGSLEKDLQEKYGEPTPQALLES
                     AMRHVDHLDRLNFDQFKVSVKASDVFLAVESYRLLAKQIDQPLHLGITEAGGARSGAV
                     KSAIGLGLLLSEGIGDTLRVSLAADPVEEIKVGFDILKSLRIRSRGINFIACPTCSRQ
                     EFDVIGTVNALEQRLEDIITPMDVSIIGCVVNGPGEALVSTLGVTGGNKKSGLYEDGV
                     RKDRLDNNDMIDQLEARIRAKASQLDEARRIDVQQVEK"
     gene            complement(12025..13038)
                     /gene="rodZ"
                     /locus_tag="ACHOSC_RS00060"
                     /old_locus_tag="pgaptmp_000012"
     CDS             complement(12025..13038)
                     /gene="rodZ"
                     /locus_tag="ACHOSC_RS00060"
                     /old_locus_tag="pgaptmp_000012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytoskeleton protein RodZ"
                     /protein_id="HGU4546030.1"
                     /translation="MNTEATHDQNEALTTGARLRNAREQLGLSQQAVAERLCLKVSTV
                     RDIEEDKAPADLASTFLRGYIRSYARLVHIPEEELLPGLEKQAPLRAAKVAPMQSFSL
                     GKRRKKRDGWLMTFTWLVLFVVIGLSGAWWWQDHKAQQEEITTMADQSSAELSSNSEQ
                     GQSVPLNTSTTTDPATTSTPPASVDTTATNTQTPAVTAPAPAVDPQQNAVVSPSQANV
                     DTAATPAPTATTTPDGAAPLPTDQAGVTTPAADPNALVMNFTADCWLEVTDATGKKLF
                     SGMQRKDGNLNLTGQAPYKLKIGAPAAVQIQYQGKPVDLSRFIRTNQVARLTLNAEQS
                     PAQ"
     gene            complement(13323..14477)
                     /locus_tag="ACHOSC_RS00065"
                     /old_locus_tag="pgaptmp_000013"
     CDS             complement(13323..14477)
                     /locus_tag="ACHOSC_RS00065"
                     /old_locus_tag="pgaptmp_000013"
                     /EC_number="2.1.1.-"
                     /EC_number="2.1.1.192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional tRNA
                     (adenosine(37)-C2)-methyltransferase TrmG/ribosomal RNA
                     large subunit methyltransferase RlmN"
                     /protein_id="HGU4546031.1"
                     /translation="MSEQLVTPENVTTKDGKINLLDLNRQQMREFFKDLGEKPFRADQ
                     VMKWMYHYCCDNFDEMTDINKVLRGKLKEVAEIRAPEVVEEQRSSDGTIKWAIAVGDQ
                     RVETVYIPEDDRATLCVSSQVGCALECKFCSTAQQGFNRNLRVSEIIGQVWRAAKIVG
                     AAKVTGQRPITNVVMMGMGEPLLNLNNVVPAMEIMLDDFGFGLSKRRVTLSTSGVVPA
                     LDKLGDMIDVALAISLHAPNDEIRDEIVPINKKYNIETFLAAVRRYLEKSNANQGRVT
                     IEYVMLDHVNDGTEHAHQLAELLKDTPCKINLIPWNPFPGAPYGRSSNSRIDRFSKVL
                     MSYGFTTIVRKTRGDDIDAACGQLAGDVIDRTKRTLRKRMQGEAIDIKAV"
     gene            complement(14627..15058)
                     /gene="ndk"
                     /locus_tag="ACHOSC_RS00070"
                     /old_locus_tag="pgaptmp_000014"
     CDS             complement(14627..15058)
                     /gene="ndk"
                     /locus_tag="ACHOSC_RS00070"
                     /old_locus_tag="pgaptmp_000014"
                     /EC_number="2.7.4.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004550 - nucleoside diphosphate kinase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-diphosphate kinase"
                     /protein_id="HGU4546032.1"
                     /translation="MAIERTFSIIKPNAVAKNVIGNIFARFEAAGFKIVGTKMLHLTV
                     EQARGFYAEHDGKPFFDGLVEFMTSGPIVVSVLEGENAVQRHRDLLGATNPANALAGT
                     LRADYADSLTENGTHGSDSVESAAREIAYFFGEGEVCPRTR"
     gene            complement(15207..17519)
                     /gene="pbpC"
                     /locus_tag="ACHOSC_RS00075"
                     /old_locus_tag="pgaptmp_000015"
     CDS             complement(15207..17519)
                     /gene="pbpC"
                     /locus_tag="ACHOSC_RS00075"
                     /old_locus_tag="pgaptmp_000015"
                     /EC_number="2.4.99.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417014.1"
                     /note="penicillin-binding protein 1C; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA];
                     GO_function: GO:0008955 - peptidoglycan
                     glycosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan glycosyltransferase PbpC"
                     /protein_id="HGU4546033.1"
                     /translation="MPRLLTKRGCWITLAAAPFLLFLAAWGADKLWPLPLHEVNPARV
                     VVAQDGTPLWRFADADGIWRYPVTIEDVSPRYLEALINYEDRWFWKHPGVNPFSVARA
                     AWQDLTSGRVISGGSTLTMQVARLLDPHPKTFGGKIRQLWRALQLEWHLSKREILTLY
                     LNRAPFGGTLQGIGAASWAYLGKSPANLSYSEAAMLAVLPQAPSRLRPDRWPERAEAA
                     RNKVLERMAVQGAWSREQVKESREEPIWLAPRQMPQLAPLFSRMMLGKSKSDKIVTTL
                     DAGLQRRLEELAQNWKGRLPPRSSLAMIVVDHTDMRVRGWVGSVDLNDDSRFGHVDMV
                     NANRSPGSVLKPFVYGLALDEGLIHPASLLQDVPRRTGDYRPGNFDSGFHGPISMSEA
                     LVRSLNLPAVQVLEAYGPKRFAAKLRNVGLPLYLPNGAAPNLSLILGGAGAKLEDMAA
                     AYTAFARHGKAGKLRLQPDDPLLERPLMSSGAAWIIRRIMADEAKPLPDSALPRVAPL
                     AWKTGTSYGYRDAWAIGVNARYVIGIWTGRPDGTPVVGQFGFASAVPLLNQVNNILLS
                     RSANLPEDPRPNSVTRGVICWPGGQSLPEGDGNCRRRLATWLLDGSQPPTLLLPEQEG
                     INGIRFPIWLDENGKRVAADCPQARQEMINVWPLPLEPWLPASERRAVRLPPASTICP
                     SYGHDAQLPLQLTGVRDGAIIKRLPGAAEATLPLQSSGGAGERWWFLNGEPLTERGRN
                     VTLHLTDKGDYQLLVMDDVGQIATVKFVMQ"
     gene            complement(17520..22482)
                     /gene="yfhM"
                     /locus_tag="ACHOSC_RS00080"
                     /old_locus_tag="pgaptmp_000016"
                     /pseudo
     CDS             complement(17520..22482)
                     /gene="yfhM"
                     /locus_tag="ACHOSC_RS00080"
                     /old_locus_tag="pgaptmp_000016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417015.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="alpha2-macroglobulin"
     gene            22689..23534
                     /gene="sseA"
                     /locus_tag="ACHOSC_RS00085"
                     /old_locus_tag="pgaptmp_000017"
     CDS             22689..23534
                     /gene="sseA"
                     /locus_tag="ACHOSC_RS00085"
                     /old_locus_tag="pgaptmp_000017"
                     /EC_number="2.8.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417016.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004792 - thiosulfate sulfurtransferase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-mercaptopyruvate sulfurtransferase"
                     /protein_id="HGU4546034.1"
                     /translation="MSTTWFVGADWLAEHIDDPEIQIIDARMASPGQEDRNVAQEYLN
                     GHIPGAVFFDIEALSDHTSPLPHMLPRPETFAVAMRELGVNQDKHLIVYDEGNLFSAP
                     RAWWMLRTFGVEKVSILGGGLAGWQRDDLLLEEGAVELPEGEFNAAFNPEAVVKVTDV
                     LLASHENTAQIIDARPAARFNAEVDEPRPGLRRGHIPGALNVPWTELVREGELKTTDE
                     LDAIFFGRGVSYDKPIIVSCGSGVTAAVVLLALATLDVPNVKLYDGAWSEWGARADLP
                     VEPVK"
     gene            23870..23995
                     /gene="timP"
                     /locus_tag="ACHOSC_RS00090"
                     /old_locus_tag="pgaptmp_000018"
     CDS             23870..23995
                     /gene="timP"
                     /locus_tag="ACHOSC_RS00090"
                     /old_locus_tag="pgaptmp_000018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010283914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="small toxic inner membrane protein TimP"
                     /protein_id="HGU4546035.1"
                     /translation="MKIRCFCIVLIVSGALFSQVNNNRSLSGDNLLVVNNLQSSK"
     gene            complement(24027..24803)
                     /gene="sseB"
                     /locus_tag="ACHOSC_RS00095"
                     /old_locus_tag="pgaptmp_000019"
     CDS             complement(24027..24803)
                     /gene="sseB"
                     /locus_tag="ACHOSC_RS00095"
                     /old_locus_tag="pgaptmp_000019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708361.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enhanced serine sensitivity protein SseB"
                     /protein_id="HGU4546036.1"
                     /translation="MSETKNELEDLLEKAATEPAHRPGFFRTLLESTVWVPGTAAQGE
                     AVVEDSALDLQHWEKEDGTSVIPFFTSLEALQQAVEDEQAFVVMPVRTLFEMTLGETL
                     FLNAKLPTGKEFMPREISLLIGGEGNPLSSQEILEGGESLILSEVAEPPAQMIDSLTT
                     LFKTIKPVKRAFICSIKENEEAQPNLLIGIEADGDIEEIIQATGSVATDTLPGDEPID
                     ICQVKKGEKGISHFITEHIAPFYERRWGGFLRDFKQNRII"
     gene            complement(24945..26228)
                     /gene="pepB"
                     /locus_tag="ACHOSC_RS00100"
                     /old_locus_tag="pgaptmp_000020"
     CDS             complement(24945..26228)
                     /gene="pepB"
                     /locus_tag="ACHOSC_RS00100"
                     /old_locus_tag="pgaptmp_000020"
                     /EC_number="3.4.11.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311416.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030145 - manganese ion binding [Evidence
                     IEA];
                     GO_function: GO:0070006 - metalloaminopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0019538 - protein metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase PepB"
                     /protein_id="HGU4546037.1"
                     /translation="MTEAMKITLSTQPADARWGEKATYSINNDGITLHLNGADDLGLI
                     QRAARKIDGLGIKHVQLSGEGWDADRCWAFWQGYKAPKGTRKVEWPDLDDAQRQELDN
                     RLMIIDWVRDTINAPAEELGPSQLAQRAVDLISNVAGDRVTYRITKGEDLREQGYMGL
                     HTVGRGSERSPVLLALDYNPTGDKEAPVYACLVGKGITFDSGGYSIKQTAFMDSMKSD
                     MGGAATVTGALAFAITRGLNKRVKLFLCCADNLISGNAFKLGDIITYRNGKKVEVMNT
                     DAEGRLVLADGLIDASAQKPEMIIDAATLTGAAKTALGNDYHALFSFDDALAGRLLAS
                     ASQENEPFWRLPLAEFHRSQLPSNFAELNNTGSAAYPAGASTAAGFLSHFVENYQQGW
                     LHIDCSATYRKAPVEQWSAGATGLGVRTIANLLTA"
     gene            complement(26406..26606)
                     /gene="iscX"
                     /locus_tag="ACHOSC_RS00105"
                     /old_locus_tag="pgaptmp_000021"
     CDS             complement(26406..26606)
                     /gene="iscX"
                     /locus_tag="ACHOSC_RS00105"
                     /old_locus_tag="pgaptmp_000021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004104502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein IscX"
                     /protein_id="HGU4546038.1"
                     /translation="MGLKWTDSREIGEALYDAYPDLDPKTVRFTDMHQWICDLEDFDD
                     DPQASNEKILEAILLVWLDEAE"
     gene            complement(26618..26953)
                     /gene="fdx"
                     /locus_tag="ACHOSC_RS00110"
                     /old_locus_tag="pgaptmp_000022"
     CDS             complement(26618..26953)
                     /gene="fdx"
                     /locus_tag="ACHOSC_RS00110"
                     /old_locus_tag="pgaptmp_000022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015683702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ISC system 2Fe-2S type ferredoxin"
                     /protein_id="HGU4546039.1"
                     /translation="MPKIVILPHQDLCPDGAVLEANSGETILDAALRNGIEIEHACEK
                     SCACTTCHCIVREGFDSLPESSEQEDDMLDKAWGLEPESRLSCQARVTDEDLVVEIPR
                     YTINHAREH"
     gene            complement(26955..28805)
                     /gene="hscA"
                     /locus_tag="ACHOSC_RS00115"
                     /old_locus_tag="pgaptmp_000023"
     CDS             complement(26955..28805)
                     /gene="hscA"
                     /locus_tag="ACHOSC_RS00115"
                     /old_locus_tag="pgaptmp_000023"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001559634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S protein assembly chaperone HscA"
                     /protein_id="HGU4546040.1"
                     /translation="MALLQISEPGLSAAPHQRRLAAGIDLGTTNSLVATVRSGQAETL
                     ADHEGRHLLPSVVHYQQQGHSVGYDARTNAALDTANTISSVKRLMGRSLADIQQRYPH
                     LPYQFQASENGLPMIETAAGLLNPVRVSADILKALAARATEALAGELDGVVITVPAYF
                     DDAQRQGTKDAARLAGLHVLRLLNEPTAAAIAYGLDSGQEGVIAVYDLGGGTFDISIL
                     RLSRGVFEVLATGGDSALGGDDFDHLLADYIREQAGIPDRSDNRVQRELLDAAIAAKI
                     ALSDADSVTVNVAGWQGEISREQFNELIAPLVKRTLLACRRALKDAGVEADEVLEVVM
                     VGGSTRVPLVRERVGEFFGRPPLTSIDPDKVVAIGAAIQADILVGNKPDSEMLLLDVI
                     PLSLGLETMGGLVEKVIPRNTTIPVARAQDFTTFKDGQTAMSIHVMQGERELVQDCRS
                     LARFALRGIPALPAGGAHIRVTFQVDADGLLSVTAMEKSTGVEASIQVKPSYGLTDSE
                     IASMIKDSMSYAEQDVKARMLAEQKVEAARVLESLHGALAADAALLSAAERQVIDDAA
                     AHLSEVAQGDDVDAIEQAIKNVDKQTQDFAARRMDQSVRRALKGHSVDEV"
     gene            complement(28822..29337)
                     /gene="hscB"
                     /locus_tag="ACHOSC_RS00120"
                     /old_locus_tag="pgaptmp_000024"
     CDS             complement(28822..29337)
                     /gene="hscB"
                     /locus_tag="ACHOSC_RS00120"
                     /old_locus_tag="pgaptmp_000024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051087 - protein-folding chaperone
                     binding [Evidence IEA];
                     GO_process: GO:0097428 - protein maturation by iron-sulfur
                     cluster transfer [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone HscB"
                     /protein_id="HGU4546041.1"
                     /translation="MDYFTLFGLPARYQLDTQALSLRFQDLQRQYHPDKFASGSQAEQ
                     LAAVQQSATINQAWQTLRHPLMRAEYLLSLHGFDLASEQHTVRDTAFLMEQLELREEL
                     DEIEQAKDEARLESFIKRVKKMFDTRHQLMVEQLDNEAWDAAADTVRKLRFLDKLRSS
                     AEQLEEKLLDF"
     gene            complement(29433..29756)
                     /gene="iscA"
                     /locus_tag="ACHOSC_RS00125"
                     /old_locus_tag="pgaptmp_000025"
     CDS             complement(29433..29756)
                     /gene="iscA"
                     /locus_tag="ACHOSC_RS00125"
                     /old_locus_tag="pgaptmp_000025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster assembly protein IscA"
                     /protein_id="HGU4546042.1"
                     /translation="MSITLSDSAAARVNTFLANRGKGFGLRLGVRTSGCSGMAYVLEF
                     VDEPTPEDIVFEDKGVKVVVDGKSLQFLDGTQLDFVKEGLNEGFKFTNPNVKDECGCG
                     ESFHV"
     gene            complement(29773..30159)
                     /gene="iscU"
                     /locus_tag="ACHOSC_RS00130"
                     /old_locus_tag="pgaptmp_000026"
     CDS             complement(29773..30159)
                     /gene="iscU"
                     /locus_tag="ACHOSC_RS00130"
                     /old_locus_tag="pgaptmp_000026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000331707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly scaffold IscU"
                     /protein_id="HGU4546043.1"
                     /translation="MAYSEKVIDHYENPRNVGSFDNNDENVGSGMVGAPACGDVMKLQ
                     IKVNDEGIIEDARFKTYGCGSAIASSSLVTEWVKGKSLDEAQAIKNTDIAEELELPPV
                     KIHCSILAEDAIKAAIADYKSKREAK"
     gene            complement(30187..31401)
                     /gene="iscS"
                     /locus_tag="ACHOSC_RS00135"
                     /old_locus_tag="pgaptmp_000027"
     CDS             complement(30187..31401)
                     /gene="iscS"
                     /locus_tag="ACHOSC_RS00135"
                     /old_locus_tag="pgaptmp_000027"
                     /EC_number="2.8.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017459011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase"
                     /protein_id="HGU4546044.1"
                     /translation="MKLPIYLDYSATTPVDPRVAEKMMQFMTMDGTFGNPASRSHRFG
                     WQAEEAVDIARNQIADLVGADPREIVFTSGATESDNLAIKGAANFYQKKGKHIITSKT
                     EHKAVLDTCRQLEREGFEVTYLAPQRNGIIDLKELEAAMRDDTILVSIMHVNNEIGVV
                     QDIAAIGEMCRARGIIYHVDATQSVGKLPIDLSQLKVDLMSFSGHKIYGPKGIGALYV
                     RRKPRVRIEAQMHGGGHERGMRSGTLPVHQIVGMGEAYRIAKEEMATEMERLRGLRNR
                     LWNGIKDIEEVYLNGDLEHGAPNILNVSFNYVEGESLIMALKDLAVSSGSACTSASLE
                     PSYVLRALGLNDELAHSSIRFSLGRFTTEEEIDYTIELVRKSIGRLRDLSPLWEMYKQ
                     GVDLNSIEWAHH"
     gene            complement(31642..32130)
                     /gene="iscR"
                     /locus_tag="ACHOSC_RS00140"
                     /old_locus_tag="pgaptmp_000028"
     CDS             complement(31642..32130)
                     /gene="iscR"
                     /locus_tag="ACHOSC_RS00140"
                     /old_locus_tag="pgaptmp_000028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002913993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly transcriptional regulator
                     IscR"
                     /protein_id="HGU4546045.1"
                     /translation="MRLTSKGRYAVTAMLDVALNSEAGPVPLADISERQGISLSYLEQ
                     LFSRLRKNGLVSSVRGPGGGYLLGKDASSIAVGEVISAVDESVDATRCQGKGGCQGGD
                     KCLTHALWRDLSDRLTGFLNNITLGELVNNQEVLDVSGRQHTHDAPRTRIQDAIDVKL
                     RA"
     gene            complement(32431..33168)
                     /gene="trmJ"
                     /locus_tag="ACHOSC_RS00145"
                     /old_locus_tag="pgaptmp_000029"
     CDS             complement(32431..33168)
                     /gene="trmJ"
                     /locus_tag="ACHOSC_RS00145"
                     /old_locus_tag="pgaptmp_000029"
                     /EC_number="2.1.1.200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (cytosine(32)/uridine(32)-2'-O)-methyltransferase TrmJ"
                     /protein_id="HGU4546046.1"
                     /translation="MLQNIRIVLVETSHTGNMGSVARAMKTMGLTNLWLVNPLVKPDS
                     QAIALAAGASDVIGNAHIVDTLDEALAGCSLVVGTSARSRTLPWPMLDPRECGLKSVA
                     EAANTPVALVFGRERVGLTNEELQKCHYHVAIAANPEYSSLNLAMAAQVIAYEVRMAW
                     LATQENGEQVEHEETPYPLVDDLERFYGHLEQTLLATGFIRENHPGQVMNKLRRLFTR
                     ARPESQELNILRGILASIEQQNKGNKA"
     gene            33287..34090
                     /gene="suhB"
                     /locus_tag="ACHOSC_RS00150"
                     /old_locus_tag="pgaptmp_000030"
     CDS             33287..34090
                     /gene="suhB"
                     /locus_tag="ACHOSC_RS00150"
                     /old_locus_tag="pgaptmp_000030"
                     /EC_number="3.1.3.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000553460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008934 - inositol monophosphate
                     1-phosphatase activity [Evidence IEA];
                     GO_process: GO:0046854 - phosphatidylinositol phosphate
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="inositol-1-monophosphatase"
                     /protein_id="HGU4546047.1"
                     /translation="MHPMLNIAVRAARKAGNLIAKNYETPDAVEASQKGSNDFVTNVD
                     KAAEAVIIDTIRKSYPQHTIITEESGELEGTDQDVQWVIDPLDGTTNFIKRLPHFAVS
                     IAVRIKGRTEVAVVYDPMRNELFTATRGQGAQLNGYRLRGSTARDLDGTILATGFPFK
                     AKQYATTYINIVGKLFNECADFRRTGSAALDLAYVAAGRVDGFFEIGLRPWDFAAGEL
                     LVREAGGIVSDFTGGHNYMLTGNIVAGNPRVVKAMLANMRDELSDALKR"
     gene            34235..35089
                     /locus_tag="ACHOSC_RS00155"
                     /old_locus_tag="pgaptmp_000031"
     CDS             34235..35089
                     /locus_tag="ACHOSC_RS00155"
                     /old_locus_tag="pgaptmp_000031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311427.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HGU4546048.1"
                     /translation="MALPVNKRVPKILFILFVVAFCVYLVPRVAINFFYYPDDKIYGP
                     DPWSAESVEFTAKDGTRLQGWFIPSSTGPADNAIATIIHAHGNAGNMSAHWPLVSWLP
                     ERNFNVFMFDYRGFGKSKGTPSQAGLLDDTQSAINVVRHRSDVNPQRLVLFGQSIGGA
                     NILAVIGQGDREGIRAVILDSTFASYATIANQMIPGSGYLLDESYSGENYIASVSPIP
                     LLLIHGKADHVIPWQHSEKLYSLAKEPKRLILIPDGEHIDAFSDRHGDVYREQMVDFI
                     LSALNPQN"
     gene            35280..36560
                     /gene="csiE"
                     /locus_tag="ACHOSC_RS00160"
                     /old_locus_tag="pgaptmp_000032"
     CDS             35280..36560
                     /gene="csiE"
                     /locus_tag="ACHOSC_RS00160"
                     /old_locus_tag="pgaptmp_000032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417030.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stationary phase inducible protein CsiE"
                     /protein_id="HGU4546049.1"
                     /translation="MMPTLAPPSVLSAPQRRCQILLTLFQPGLTATMATFSELNGVDD
                     DIASLDISETGREILRYHQLTLTTGYDGSYRVEGTVLNQRLCLFHWLRRGFRLCPSFI
                     TSQFTPALKSELKRRGIARNFYDDTNLQALVNLCSRRLQKRFESRDIHFLCLYLQYCL
                     LQHHAGITPQFNPLQRRWAESCLEFQVAQEIGRHWQRRALQPVPPDEPLFMALLFSML
                     RVPDPLRDAHQRDRQLRQSIKRLVNHFRELGNVRFYDEQGLCDQLYTHLAQALNRSLF
                     AIGIDNTLPEEFARLYPRLVRTTRAALAGFESEYGVHLSDEESGLVAVIFGAWLMQEN
                     DLHEKQIILLTGNDSEREAQIEQQLRELTLLPLNIKHMSVKAFLQTGAPRGAALIIAP
                     YTMPLPLFSPPLIYTDLTLTTHQQEQIRKMLESA"
     gene            complement(36552..37691)
                     /locus_tag="ACHOSC_RS00165"
                     /old_locus_tag="pgaptmp_000033"
     CDS             complement(36552..37691)
                     /locus_tag="ACHOSC_RS00165"
                     /old_locus_tag="pgaptmp_000033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-phenylpropionate MFS transporter"
                     /protein_id="HGU4546050.1"
                     /translation="MVLQSTRWLALGYFTYFFSYGIFLPFWSVWLKGIGLTPETIGLL
                     LGAGLVARFLGSLLIAPRVSDPSRLISALRVLALLTLLFAVAFWAGAHVAWLMLVMIG
                     FNLFFSPLVPLTDALANTWQKQFPLDYGKVRLWGSVAFVIGSALTGKLVTMFDYRVIL
                     ALLTLGVASMLLGFLIRPTIQPQGASRQQESTGWSAWLALVRQNWRFLACVCLLQGAH
                     AAYYGFSAIYWQAAGYSASAVGYLWSLGVVAEVIIFALSNKLFRRCSARDMLLISAIC
                     GVVRWGIMGATTALPWLIVVQILHCGTFTVCHLAAMRYIAARQGSEVIRLQAVYSAVA
                     MGGSIAIMTVFAGFLYQYLGHGVFWVMALVALPAMFLRPKVVPSC"
     gene            complement(37851..38741)
                     /gene="hcaR"
                     /locus_tag="ACHOSC_RS00170"
                     /old_locus_tag="pgaptmp_000034"
     CDS             complement(37851..38741)
                     /gene="hcaR"
                     /locus_tag="ACHOSC_RS00170"
                     /old_locus_tag="pgaptmp_000034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator HcaR"
                     /protein_id="HGU4546051.1"
                     /translation="MELRHLRYFVAVAQALNFTRAAEKLHTSQPSLSSQIRDLENCVG
                     VPLLVRDKRKVALTAAGECFLQDALAILEQAENAKLRARKIVQEDRQLTIGFVPSAEV
                     NLLPKVLPMFRLRQPDTLIELVSLITTQQEEKIRRGELDVGLMRHPVYSPEIDYLELF
                     DEPLVVVLPVDHPLAHEKEITAAQLDGVNFVSTDPVYSGSLAPIVKAWFAQENSQPNI
                     VQVATNILVTMNLVGMGLGVTLIPGYMNNFNTGQVVFRPIAGNVPSIALLMAWKKGEM
                     KPALRDFIAIVQERLASVTA"
     gene            38877..40238
                     /gene="hcaE"
                     /locus_tag="ACHOSC_RS00175"
                     /old_locus_tag="pgaptmp_000035"
     CDS             38877..40238
                     /gene="hcaE"
                     /locus_tag="ACHOSC_RS00175"
                     /old_locus_tag="pgaptmp_000035"
                     /EC_number="1.14.12.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008695 - 3-phenylpropionate dioxygenase
                     activity [Evidence IEA];
                     GO_process: GO:0019380 - 3-phenylpropionate catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-phenylpropionate/cinnamic acid dioxygenase
                     subunit alpha"
                     /protein_id="HGU4546052.1"
                     /translation="MTTPSDLNIYQLIDTQNGRVTPRIYTDPDIYQLELERIFGRCWL
                     FLAHESQIPKPGDFFNTYMGEDAVVVVRQKDGSIKAFLNQCRHRAMRVSYADCGNTRA
                     FTCPYHGWSYGINGELIDVPLEPRAYPQGLCKSHWGLNEVPCVESYKGLIFGNWDTSA
                     PGLRDYLGDIAWYLDGMLDRREGGTEIVGGVQKWVINCNWKFPAEQFASDQYHALFSH
                     ASAVQVLGAKDDGSDKRLGDGQTARPVWETAKDALQFGQDGHGSGFFFTEKPDANVWV
                     DGAVSSYYRETYAEAEQRLGEVRALRLAGHNNIFPTLSWLNGTATLRVWHPRGPDQVE
                     VWAFCITDKAASDEVKAAFENSATRAFGPAGFLEQDDSENWCEIQKLLKGHRARNSKL
                     CLEMGLGQEKRRDDGIPGITNYIFSETAARGMYQRWADLLSSESWQEVLDKTAAYQQE
                     VMK"
     gene            40235..40753
                     /gene="hcaF"
                     /locus_tag="ACHOSC_RS00180"
                     /old_locus_tag="pgaptmp_000036"
     CDS             40235..40753
                     /gene="hcaF"
                     /locus_tag="ACHOSC_RS00180"
                     /old_locus_tag="pgaptmp_000036"
                     /EC_number="1.14.12.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008695 - 3-phenylpropionate dioxygenase
                     activity [Evidence IEA];
                     GO_process: GO:0019380 - 3-phenylpropionate catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-phenylpropionate/cinnamic acid dioxygenase
                     subunit beta"
                     /protein_id="HGU4546053.1"
                     /translation="MSAQVSLELHHRISQFLFHEASLLDDWKFRDWLAQLDEEIRYTM
                     RTTVNAQTRDRRKGVQPPTTWIFNDTKDQLERRIARLETGMAWAEEPPSRTRHLISNC
                     QISETDIPNVFAVRVNYLLYRAQKERDETFYVGTRFDKVRRLEDDNWRLLERDIVLDQ
                     AVITSHNLSVLF"
     gene            40753..41073
                     /locus_tag="ACHOSC_RS00185"
                     /old_locus_tag="pgaptmp_000037"
     CDS             40753..41073
                     /locus_tag="ACHOSC_RS00185"
                     /old_locus_tag="pgaptmp_000037"
                     /EC_number="1.14.12.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 3-phenylpropionate/cinnamic acid
                     dioxygenase ferredoxin subunit"
                     /protein_id="HGU4546054.1"
                     /translation="MNRIYACPVADVPEGEALRIDTSPVIALFNVGGEFYAINDRCSH
                     GNASMSEGYLEDDATVECPLHAASFCLKTGKALCLPATDPLTTYPVHVEGGDIFIDLP
                     EAQP"
     gene            41070..41882
                     /gene="hcaB"
                     /locus_tag="ACHOSC_RS00190"
                     /old_locus_tag="pgaptmp_000038"
                     /gene_synonym="phdD"
                     /gene_synonym="yfhX"
     CDS             41070..41882
                     /gene="hcaB"
                     /locus_tag="ACHOSC_RS00190"
                     /old_locus_tag="pgaptmp_000038"
                     /gene_synonym="phdD"
                     /gene_synonym="yfhX"
                     /EC_number="1.3.1.87"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-phenylpropionate-dihydrodiol/cinnamic
                     acid-dihydrodiol dehydrogenase"
                     /protein_id="HGU4546055.1"
                     /translation="MSDLHNESIFITGGGSGLGLALVERFIEEGAQVATLELSAAKVA
                     SLRQRFGEHILAVEGNVTCYADYQRAVDQILTRSGKLDCFIGNAGIWDHNASLVNTPA
                     ETLETGFHELFNVNVLGYLLGAKACAPALIASEGSMIFTLSNAAWYPGGGGPLYTASK
                     HAATGLIRQLAYELAPKVRVNGVGPCGMASDLRGPQALGQSETSIMQSLTPEKIAAIL
                     PLQFFPQPADFTGPYVMLASRRNNRALSGVMINADAGLAIRGIRHVAAGLDL"
     gene            41892..43094
                     /gene="hcaD"
                     /locus_tag="ACHOSC_RS00195"
                     /old_locus_tag="pgaptmp_000039"
     CDS             41892..43094
                     /gene="hcaD"
                     /locus_tag="ACHOSC_RS00195"
                     /old_locus_tag="pgaptmp_000039"
                     /EC_number="1.14.12.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylpropionate dioxygenase ferredoxin
                     reductase subunit"
                     /protein_id="HGU4546056.1"
                     /translation="MKEKTIIIVGGGQAAAMAAASLRQQGFTGELHLFSDERHLPYER
                     PPLSKSMLLEDSPQLQQVLPANWWQENNVHLHSGVTIKTLGRDTRELVLTNGESWHWD
                     QLFIATGAAARPLPLLDALGERCFTLRHAGDAARLREVLQPERSVVIVGAGTIGLELA
                     ASATQRRCKVTVIELAATVMGRNAPPPVQRYLLQRHQQAGVRILLNNAIEHVVDGEKV
                     ELTLQSGETLQADVVIYGIGISANEQLAREANLDTANGIVIDEACRTCDPAIFAGGDV
                     AITRLDNGALHRCESWENANNQAQIAAAAMLGLPLPLLPPPWFWSDQYSDNLQFIGDM
                     RGDDWLCRGNPETQKAIWFNLQNGVLIGAVTLNQGREIRPIRKWIQSGKTFDAKLLID
                     ENIALKSL"
     gene            43191..43613
                     /locus_tag="ACHOSC_RS00200"
                     /old_locus_tag="pgaptmp_000040"
     CDS             43191..43613
                     /locus_tag="ACHOSC_RS00200"
                     /old_locus_tag="pgaptmp_000040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417038.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DoxX family protein"
                     /protein_id="HGU4546057.1"
                     /translation="MNTLRYFDFGAARPVLLLIARIAVVLIFIIFGFPKMMGFDGTVQ
                     YMASLGAPMPMLAAIIAVVMEVPAAILIVLGFFTRPLAVLFIFYTLGTAVIGHHYWDM
                     TGDAVGPNMINFWKNVSIAGAFLLLAITGPGAISLDRR"
     gene            complement(43661..44533)
                     /locus_tag="ACHOSC_RS00205"
                     /old_locus_tag="pgaptmp_000041"
     CDS             complement(43661..44533)
                     /locus_tag="ACHOSC_RS00205"
                     /old_locus_tag="pgaptmp_000041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldose 1-epimerase"
                     /protein_id="HGU4546058.1"
                     /translation="MTIYTLSHGSLKLDVSDQGGVIEGFWRDTTPLLRPGKKSGVATD
                     ASCFPLVPFANRVSGNRFVWQGREYQLQPNVEWDAHYLHGDGWLGEWQCVSHSDDSLC
                     LVYEHRSGVYHYRVSQAFHLTADTLTVTLSVTNQGAETLPFGTGWHPYFPLSPQTRIQ
                     AQASGYWLEREQWLAGEFCEQLPQELDFNQPAPLPRQWVNNGFAGWNGQARIEQPQEG
                     YAIIMETTPPAPCYFIFVSDPAFDKGYAFDFFCLEPMSHAPDDHHRPEGGDLIALAPG
                     ESTTSEMSLRVEWL"
     gene            complement(44545..45639)
                     /locus_tag="ACHOSC_RS00210"
                     /old_locus_tag="pgaptmp_000042"
     CDS             complement(44545..45639)
                     /locus_tag="ACHOSC_RS00210"
                     /old_locus_tag="pgaptmp_000042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding dehydrogenase"
                     /protein_id="HGU4546059.1"
                     /translation="MKINRIVNEGFMKTMLAAYLPGNSTVDLREVAVPTPGINQVLIK
                     MKSSGICGSDVHYIYHQHRATAAAPDKPLYQGFINGHEPCGQIVAMGQGCRHFKEGDR
                     VLVYHISGCGFCPNCRRGFPISCTGKGKAAYGWQRDGGHAEYLLAEEKDLILLPDALS
                     YEDGAFISCGVGTAYEGILRGEVSGSDNVLVVGLGPVGMMAMMLAKGRGAKRIIGVDM
                     LPERLAMAKQLGVMDHGYLATTEGLPQIIAELTHGGADVALDCSGNAAGRLLALRSTA
                     DWGRVVYIGETGKVEFEVSADLMHHQRRIIGSWVTSLFHMEKCAHDLTDWKLWPRNAI
                     THRFSLEQAGDAYALMASGKCGKVVINFPD"
     gene            complement(45672..46670)
                     /locus_tag="ACHOSC_RS00215"
                     /old_locus_tag="pgaptmp_000043"
     CDS             complement(45672..46670)
                     /locus_tag="ACHOSC_RS00215"
                     /old_locus_tag="pgaptmp_000043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HGU4546060.1"
                     /translation="MSASSLPLPQGKSVSLKQFVSRHINEIGLLVVIAILYLVFSLNA
                     PGFISLNNQMNVLRDAATIGIAAWAMTLIIISGEIDVSVGPMVAFVSVCLAFLLQFEV
                     PLAVACLLVLLLGALMGTLAGVLRGVFNVPSFVATLGLWSALRGMGLFMTNALPVPID
                     ENEVLDWLGGQFLGVPVSALIMMVLFALFVFISRKTAFGRSVFAVGGNATAAQLCGIN
                     VRRVRILIFTLSGLLAAVTGILLAARLGSGNAGAANGLEFDVIAAVVVGGTALSGGRG
                     SLFGTLLGVLVITLIGNGLVLLGINSFFQQVVRGVIIVVAVLANILLTQRSSKAKR"
     gene            complement(46695..48206)
                     /locus_tag="ACHOSC_RS00220"
                     /old_locus_tag="pgaptmp_000044"
     CDS             complement(46695..48206)
                     /locus_tag="ACHOSC_RS00220"
                     /old_locus_tag="pgaptmp_000044"
                     /EC_number="7.5.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter ATP-binding protein"
                     /protein_id="HGU4546061.1"
                     /translation="MFTATEAVPVAKVVAGNKRYPGVVALDNVNFTLNKGEVRALLGK
                     NGAGKSTLIRMLTGSERPDSGDIWIGETRLEGDEATLTRRAAELGVRAVYQELSLVEG
                     LTVAENLCLGQWPRRNGMIDYLQMAQDAQRCLQALGVDVSPEQLVSTLSPAQKQLVEI
                     ARVMKGEPRVVILDEPTSSLASAEVELVIIAVKKMSALGVAVIYVSHRMEEIRRIASC
                     ATVMRDGQVAGDVMLENTSTHHIVSLMLGRDHVDIAPVAPQEIVDQAVLEVRALRHKP
                     KLEDISFTLRRGEVLGIAGLLGAGRSELLKAIVGLEEYEQGEIVINGEKITRPDYGDM
                     LKRGIGYTPENRKEAGIIPWLGVDENTVLTNRQKISANGVLQWSTIRRLTEEVMQRMT
                     VKAASSETPIGTLSGGNQQKVVIGRWVYAASQILLLDEPTRGVDIEAKQQIYRIVREL
                     AAEGKSVVFISSEVEELPLVCDRILLLQHGTFSQEFHAPVNVDELMSAILSVH"
     gene            complement(48229..49212)
                     /locus_tag="ACHOSC_RS00225"
                     /old_locus_tag="pgaptmp_000045"
     CDS             complement(48229..49212)
                     /locus_tag="ACHOSC_RS00225"
                     /old_locus_tag="pgaptmp_000045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HGU4546062.1"
                     /translation="MPKKMRTTRNLLLMATLLGSALFARAADKEMTIGAIYLDTQGYY
                     AGVRQGVQDAAKDSSVQVQLIETNAQGDISKESTFVDTLVARNVDAIILSAVSENGSS
                     RTVRRASEAGIPVICYNTCINQKGGDKYVSAYLVGDPLEFGKKLGNAAADYFIANKID
                     QPKIAVINCEAFEVCVQRRKGFEEVLKSRVPGAQIVANQEGTVLDKAISVGEKLIIST
                     PDLNAIMGESGGATLGAVKAVRNQNQAGKIAVFGSDMTTEIAQELENNQVLKAVVDIS
                     GKKMGNAVFAQTLKVINKQADGEKVIQVPIDLYTKTEDGKQWLATHVDGLP"
     gene            complement(49309..52590)
                     /locus_tag="ACHOSC_RS00230"
                     /old_locus_tag="pgaptmp_000046"
     CDS             complement(49309..52590)
                     /locus_tag="ACHOSC_RS00230"
                     /old_locus_tag="pgaptmp_000046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417044.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5107 domain-containing protein"
                     /protein_id="HGU4546063.1"
                     /translation="MTPVKVWQERVEIPTYETGPQDIHPMFLENRVYQGSSGAVYPYG
                     VTDTLSEQKTLKSWQAVWLENDYIKVMILPELGGRVHRAWDKVKQRDFVYHNEVIKPA
                     LVGLLGPWISGGIEFNWPQHHRPTTFMPVDFTLEAHEDGAQTVWVGETEPMHGLQVMT
                     GFTLRPDRAALEIASRVYNGNATPRHFLWWANPAVKGGEGHQSVFPPDVTAVFDHGKR
                     AVSAFPIATGTYYKVDYSAGVDISRYKNVPVPTSYMAEKSQYDFVGAWCHDEDGGLLH
                     VANHHIAPGKKQWSWGHSEFGQAWDKSLTDNNGPYIELMTGIFADNQPDFTWLDAYEE
                     KRFEQYFLPYHSLGMVQNASRDAVIKLQRSKRGIEWGLYAISPLNGYRLAIREIGKCN
                     ALLDDAVALMPATAIQGVLHGINPERLTIELSDADGNIVLSYQEHQPQELPLPDVAKA
                     PLAAQDITSTDEAWFIGQHLEQYHHASRSPFDYYLRGVALDPLDYRCNLALAMLEYNR
                     ADFPQAVAYATQALKRAHALNKNPQCGQASLIRASAYERQGQYQQAEEDFWRAVWSGN
                     SKAGGYYGLARLAARNGNFDAGLDFCQQSLRACPTNQEVLCLHNLLLVLSGRQDNARV
                     QREKLLRDYPLNATLWWLNWFDGRSESALAQWRGLCQGRDVNALMTAGQLINWGMPTL
                     AAEMLNALDCQRTLPLYLQASLLPKAERGELVAKAIDVFPQFVRFPNTLEEVAALESI
                     EECWFARHLLACFYYNKRSYNKAIALWQRCVEMSPEFADGWRGLAIHAWNKQHDYELA
                     ARYLDNAYQLAPQDARLLFERDLLDKLSGATPEKRLARLENNQEIALKRDDMTAELLN
                     LWHLTGQADKAADILATRKFHPWEGGEGKVTSQFILNQLLRAWQHLDAREPQQASELL
                     HAALHYPENLSEGRLPGQTDNDIWFWQAICANAQGDETEAMRCLRLAATGDRTINIHS
                     YYNDQPVDYLFWQGMALRLLGEQQTAQQLFSEMKQWAQEMAKTSIEADFFAVSQPDLL
                     SLYGDLQQQHKEKCLMVAMLASAGLGEVAQYESARAELTAINPAWPKAALFTTVMPFI
                     FNYVH"
     gene            52708..53901
                     /locus_tag="ACHOSC_RS00235"
                     /old_locus_tag="pgaptmp_000047"
     CDS             52708..53901
                     /locus_tag="ACHOSC_RS00235"
                     /old_locus_tag="pgaptmp_000047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417045.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HGU4546064.1"
                     /translation="MRACINNQQIRHHNKCVILELLYRQKRANKSTLARLAQISIPAV
                     SNILQELESEKRVVNIDDESQTRGHSSGTWLIAPEGDWTLCLNVTPTSIECQVANACL
                     SPKGEFEYLQIDAPTPQALLSEIEKCWHRHRKLWPDRTINLALAIHGQVDPVTGVSQT
                     MPQAPWTTPVEVKYLLEEKLGIRVMVDNDCVMLALAEKWQNNSQERDFCVINVDYGIG
                     SSFVINEQIYRGSLYGSGQIGHTIVNPDGVVCDCGRYGCLETVASLSALKKQARVWLK
                     SQPVSTQLDPEKLTTAQLIAAWQSGEPWITSWVDRSANAIGLSLYNFLNILNINQIWL
                     YGRSCAFGENWLNTIIRQTGFNPFDRDEGPSVKATQIGFGQLSRAQQVLGIGYLYVEA
                     QLRQI"
     gene            complement(53965..55218)
                     /gene="glyA"
                     /locus_tag="ACHOSC_RS00240"
                     /old_locus_tag="pgaptmp_000048"
     CDS             complement(53965..55218)
                     /gene="glyA"
                     /locus_tag="ACHOSC_RS00240"
                     /old_locus_tag="pgaptmp_000048"
                     /EC_number="2.1.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019077397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydroxymethyltransferase"
                     /protein_id="HGU4546065.1"
                     /translation="MLKREMNIADYDAELWQAMEQEKVRQEEHIELIASENYTSPRVM
                     QAQGSQLTNKYAEGYPGKRYYGGCEYVDIVEQLAIDRAKELFGADYANVQPHSGSQAN
                     FAVYTALLEPGDTVLGMNLAHGGHLTHGSPVNFSGKLYNIVPYGIDATGHIDYADLEK
                     QAKEHKPKMIIGGFSAYSGVVDWAKMREIADSIGAYLFVDMAHVAGLVAAGVYPNPVP
                     HAHVVTTTTHKTLAGPRGGLILAKGGSEELYKKLNSAVFPGGQGGPLMHVIAGKAVAL
                     KEAMEPEFKTYQQQVAKNAKAMVEVFLERGYKVVSGGTDNHLFLVDLVDKNLTGKEAD
                     AALGRANITVNKNSVPNDPKSPFVTSGIRVGTPAITRRGFKEAEAKELAGWMCDVLDS
                     INDEAVIERIKGKVLDICARYPVYA"
     gene            55546..56736
                     /gene="hmpA"
                     /locus_tag="ACHOSC_RS00245"
                     /old_locus_tag="pgaptmp_000049"
     CDS             55546..56736
                     /gene="hmpA"
                     /locus_tag="ACHOSC_RS00245"
                     /old_locus_tag="pgaptmp_000049"
                     /EC_number="1.14.12.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008941 - nitric oxide dioxygenase NAD(P)H
                     activity [Evidence IEA];
                     GO_function: GO:0019825 - oxygen binding [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA];
                     GO_process: GO:0051409 - response to nitrosative stress
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NO-inducible flavohemoprotein"
                     /protein_id="HGU4546066.1"
                     /translation="MLDAQTIATVKATIPLLVETGPKLTAHFYDRMFTHNPELKEIFN
                     MSNQRNGDQREALFNAIAAYASNIENLPALLPAVEKIAQKHTSFQIKPEQYNIVGEHL
                     LATLDEMFSPGQEVLDAWGKAYGVLANVFINREAEIYNENASKAGGWEGTRDFRIVAK
                     TPRSALITSFELEPVDGGAVAEYRPGQYLGVWLKPEGFPHQEIRQYSLTRKPDGKGYR
                     IAVKREEGGQVSNWLHNHANVGDVVKLVAPAGDFFMAVADDTPVTLISAGVGQTPMLA
                     MLDTLAKAGHTAQVNWFHAAENGDVHAFADEVKELGQSLPRFTAHTWYRQPSEADRAK
                     GQFDSEGLMDLSKLEGAFSDPTMQFYLCGPVGFMQFTAKQLVDLGVKQENIHYECFGP
                     HKVL"
     gene            complement(56781..57119)
                     /gene="glnB"
                     /locus_tag="ACHOSC_RS00250"
                     /old_locus_tag="pgaptmp_000050"
     CDS             complement(56781..57119)
                     /gene="glnB"
                     /locus_tag="ACHOSC_RS00250"
                     /old_locus_tag="pgaptmp_000050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017373484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrogen regulatory protein P-II"
                     /protein_id="HGU4546067.1"
                     /translation="MKKIDAIIKPFKLDDVREALAEVGITGMTVTEVKGFGRQKGHTE
                     LYRGAEYMVDFLPKVKIEIVVPDDIVDTCVDTIIRTAQTGKIGDGKIFVFDVARVIRI
                     RTGEEDDAAI"
     gene            complement(57180..58514)
                     /gene="glrR"
                     /locus_tag="ACHOSC_RS00255"
                     /old_locus_tag="pgaptmp_000051"
     CDS             complement(57180..58514)
                     /gene="glrR"
                     /locus_tag="ACHOSC_RS00255"
                     /old_locus_tag="pgaptmp_000051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator GlrR"
                     /protein_id="HGU4546068.1"
                     /translation="MSHKPAHLLLVDDDPGLLKLLGLRLTSEGYSVVTAESGAEGLRV
                     LNREKVDLVISDLRMDEMDGMQLFAEIQKVQPGMPVIILTAHGSIPDAVAATQQGVFS
                     FLTKPVDKDALYQAIDDALEQSAPATDERWREAIVTRSPLMLRLLEQARLVAQSDVSV
                     LINGQSGTGKEIFAQAIHNASPRNSKPFIAINCGALPEQLLESELFGHARGAFTGAVS
                     NREGLFQAAEGGTLFLDEIGDMPAPLQVKLLRVLQERKVRPLGSNRDIDIDVRIISAT
                     HRDLPKAMARGEFREDLYYRLNVVSLKIPALAERTEDIPLLANHLLRQAAERHKPFVR
                     AFSTDAMKRLMTASWPGNVRQLVNVIEQCVALTSSPVISDALVEQALEGENTALPTFV
                     EARNQFELNYLRKLLQITKGNVTHAARMAGRNRTEFYKLLSRHELDANDFKE"
     gene            complement(58504..59217)
                     /gene="qseG"
                     /locus_tag="ACHOSC_RS00260"
                     /old_locus_tag="pgaptmp_000052"
     CDS             complement(58504..59217)
                     /gene="qseG"
                     /locus_tag="ACHOSC_RS00260"
                     /old_locus_tag="pgaptmp_000052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system QseEF-associated
                     lipoprotein QseG"
                     /protein_id="HGU4546069.1"
                     /translation="MRHIFQRLLPRRLWLAGLPCLALLGCVQSHNKPAIDTPAEEKIP
                     VYQLADYLSTECSDIWALQGKSTETNPLYWLRAMDCADRLMPAQSRQQARQYDDGSWQ
                     NTFKQGILLADAKITPYERRQLVARIDALSTEIPAQVRPLYQLWRDGQALQLQLAEER
                     QRYGKLQQASDSELDTLRQQHHVLQQQLELTTRKLENLTDIERQLSTRKPAGNFSPDT
                     PHEGEKPAPSTHEVTPDEP"
     gene            complement(59382..60809)
                     /gene="qseE"
                     /locus_tag="ACHOSC_RS00265"
                     /old_locus_tag="pgaptmp_000053"
     CDS             complement(59382..60809)
                     /gene="qseE"
                     /locus_tag="ACHOSC_RS00265"
                     /old_locus_tag="pgaptmp_000053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417051.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two component system sensor histidine kinase
                     QseE/GlrK"
                     /protein_id="HGU4546070.1"
                     /translation="MKRWPVFPRSLRQLVMLAFLLILLPLLVLAWQAWQSLNALSDQA
                     ALVNRTTLIDARRSEAMTNAALEMERSYRQYCVLDDPTLAKVYQSQRKRYSEMLDAHA
                     GVLPDDKLYQALRQDLNNLAQLQCNNSGPDAAAAARLEAFASANTEMVQATRTVVFSR
                     GQQLQREIAERGQYFGWQSLVLFLVSLVMVLLFTRMIIGPVKNIERMINRLGEGRSLG
                     NSVSFSGPSELRSVGQRILWLSERLSWLESQRHQFLRHLSHELKTPLASMREGTELLA
                     DQVVGPLTPEQKEVVSILDSSSRNLQKLIEQLLDYNRKQADSAVELENVELAPLVETV
                     VSAHSLPARAKMMHTDVDLKATACLAEPMLLMSVLDNLYSNAVHYGAESGNICLRSSL
                     HGARVYIDVINTGTPIPQEERAMIFEPFFQGSHQRKGAVKGSGLGLSIARDCICRMQG
                     ELYLVDESGQDVCFRIELPSSKNTK"
     gene            complement(61385..65272)
                     /gene="purL"
                     /locus_tag="ACHOSC_RS00270"
                     /old_locus_tag="pgaptmp_000054"
                     /gene_synonym="purI"
     CDS             complement(61385..65272)
                     /gene="purL"
                     /locus_tag="ACHOSC_RS00270"
                     /old_locus_tag="pgaptmp_000054"
                     /gene_synonym="purI"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004642 -
                     phosphoribosylformylglycinamidine synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase"
                     /protein_id="HGU4546071.1"
                     /translation="MMEILRGSPALSAFRINKLLARFQAARLPVHNIYAEYVHFADLN
                     APLNDDEHAQLERLLKYGPALASHAPQGKLLLVTPRPGTISPWSSKATDIAHNCGLQQ
                     VNRLERGVAYYIEAGTLTNEQWQQVTAELHDRMMETVFFALDDAEQLFAHHQPTPVTS
                     VDLLGQGRQALIDANLRLGLALAEDEIDYLQDAFTKLGRNPNDIELYMFAQANSEHCR
                     HKIFNADWVIDGEQQPKSLFKMIKNTFETTPDYVLSAYKDNAAVMEGSEVGRYFADHE
                     TGRYDFHQEPAHILMKVETHNHPTAISPWPGAATGSGGEIRDEGATGLGAKPKAGLVG
                     FSVSNLRIPGFEQPWEEDFGKPERIVTALDIMTEGPLGGAAFNNEFGRPALNGYFRTY
                     EEKVNSHNGEELRGYHKPIMLAGGIGNIRADHVQKGEINVGAKLVVLGGPAMNIGLGG
                     GAASSMASGQSDADLDFASVQRDNPEMERRCQEVIDRCWQLGDANPILFIHDVGAGGL
                     SNAMPELVSDGGRGGKFELRDILSDEPGMSPLEIWCNESQERYVLAVAADQLPLFDEL
                     CKRERAPYAVIGEATEELHLSLHDRHFDNQPIDLPLDVLLGKTPKMTRDVQTLKAKGD
                     ALVREGITIADAVKRVLHLPTVAEKTFLVTIGDRSVTGMVARDQMVGPWQVPVANCAV
                     TTASLDSYYGEAMAIGERAPVALLDFAASARLAVGEALTNIAATQIGDIKRIKLSANW
                     MAAAGHPGEDAGLYEAVKAVGEELCPALGLTIPVGKDSMSMKTRWQEGNEEREMTSPL
                     SLVISAFARVEDVRHTITPQLSTEDNALLLIDLGKGNNALGATALAQVYRQLGDKPAD
                     VRDVAQLKGFYDAIQALVAQRKLLAYHDRSDGGLLVTLAEMAFAGHCGIDADIGSLGD
                     DRLAALFNEELGAVIQVRAADREAVESVLAQHGLADCVHYVGQAVSGDRFVITANGQT
                     VFSESRTTLRVWWAETTWQMQRLRDNPECADQEHQAKSNDADPGLNVKLSFDINEDVA
                     APFIATGARPKVAVLREQGVNSHVEMAAAFHRAGFDAIDVHMSDLLAGRTGLEDFHAL
                     VACGGFSYGDVLGAGEGWAKSILFNDRVRDEFATFFHRPQTLALGVCNGCQMMSNLRE
                     LIPGSELWPRFVRNTSDRFEARFSLVEVTQSPSLLLQGMVGSQMPIAVSHGEGRVEVR
                     DAAHLAALESKGLVALRYVDNFGKVTETYPANPNGSPNGITSVTTESGRVTIMMPHPE
                     RVFRTVSNSWHPENWGEDGPWMRIFRNARKQLG"
BASE COUNT        15425 a        18174 c        16552 g        15361 t
ORIGIN      
        1 aatgtcttct gcctgtggca atgttagcgt cattcactct ggcaggatgc cagtcaaatg
       61 ctgacgatca tgccgccgat gtttatcaaa ccgatcaact gaataccaaa caagaaacta
      121 aaaccgttaa tattatttcc attcttcccg caaaagttgc cgtagacaac tcccaaaata
      181 aacggaacgc acaagccttc ggcgcgctta ttggcgcagt cgctggcggt gttatcggcc
      241 acaacgtcgg ttctggcagc aattccggaa cgacggcagg ggcagttggc ggcggagctg
      301 taggcgcggc agtgggttct atggtgaatg ataaaacctt agtggaaggt gtttctttaa
      361 catataagga aggcaccaaa gtgtatacct ctacccaggt gggtaaagag tgccagttta
      421 cgacaggttt agccgttgtt attaccacaa cgtataacga aacgcgtatt cagccaaata
      481 ctaaatgtcc tgaaaagagc taataatcag gaggagtcat gaagaaagtt tttctttgcg
      541 ccatcttggc ctccttaagc tatccggcta tcgcctcatc attgcaggat caactctctg
      601 ctgtagcaga agctgaacag caaggtaaaa atgaagagca aaggcagcat gacgaatggg
      661 tcgcggagcg caacagggaa atccagcaag agaagcaacg tcgcgcaaat gcccaggccg
      721 ccgctaacaa aagagcggca acggcagcgg caaataagaa agctcgtcag gataaactgg
      781 acgccgaagc ctctgcggac aaaaaacgcg atcaaagtta tgaagatgag ctacgcagct
      841 tagagattca gaaacaaaaa ctggcgctgg caaaagaaga agcccgcgtt aagcgagaaa
      901 acgaatttat cgatcaggaa ctgaagcaca aagctgcgca aaccgatgtg gtgcaatctg
      961 aagctgacgc caacagaaat atgactgaag gcggtcgcga tctgatgaaa cgcgtgggca
     1021 aagcagaaga gaacaaatcg gacagctggt ttaattaatc gatgttagta acttcaagcc
     1081 tatgattctt gaagataaaa aaccctctgt agtaacagag ggttttgttc attcatagtg
     1141 cagggattaa aatcattccc actcaatggt agctggcggc ttgccgctga tgtcatacac
     1201 cacgcgggaa ataccgttca cttcattgat aatgcggttg gaaacgcgac cgaggaaatc
     1261 gtacggcaga tgcgcccagt gtgcggtcat aaagtcgatg gtttcgacag cacgcagaga
     1321 gacaacccaa tcatacttac gaccatcgcc cattacgcca acggaacgta ccggcaggaa
     1381 cacagtgaac gcctggctga ctttgtcgta caggtccgct ttacgcagtt cttcaatgaa
     1441 gatggcgtca gcacggcgca gcaggtcaca gtactctttc ttcacttcac ccagcacacg
     1501 aacgccaagg cctggtcccg ggaacgggtg acggtacagc atgtcgtacg gcaggcccag
     1561 ctccagacca atcttacgca cttcgtcttt gaacagctct ttcagcggtt caaccaggcc
     1621 catcttcatc tctttcggca ggccgcccac gttgtggtga gatttgatga cgtgtgcttt
     1681 accggttgca gaagccgcag attcgataac gtcagggtag atggtgccct gcgccagcca
     1741 cttcacgtct tccagtttca gcgcttcttc atcgaatact tcaacgaaaa cgcgcccgat
     1801 gattttacgt tttgcttccg gatcgttttc gccagccagc gctgacagga agcgatcttc
     1861 tgccggtacg tgaacaatgt taagaccaaa gtgatcgcca aacatatcca gaacctgctc
     1921 tgcttcgttg agacgcagca ggccgttgtc gacgaatacg caagtcaggt ttttaccgat
     1981 agcgcggtgc agcagcattg cggttacgga ggaatccaca ccaccagaga ggccgaggat
     2041 gactttatcg tcgcctacct gctcgcggat gcgagctaca gcatcgtcga taattttcgc
     2101 tggcgtccac agggcttcac actggcagat atcacgcaca aaacgctcca gcatgcgcat
     2161 accctggcgg gtgtgagtca cttccgggtg gaactgtacg ccatagaagc gtttttcttc
     2221 gttagccata atggcaaacg ggcagctttc ggtgctggct acggtgacga agtcggacgg
     2281 gatagcggta actttatcgc cgtggctcat ccagacatcg agcagcggtt taccgtctgc
     2341 ggtcagcgca tcttcgatac cgcgaaccag tgcgctgtcg tttacgactt caacctgcgc
     2401 gtagccaaat tcacgttcgt tagaggcttc aacgtgaccg cccaactgca ttgccatggt
     2461 ctgcatgcca tagcaaacgc cgaataccgg tacgcctgct tcaaagacat actgcggcgc
     2521 acgcgggctg ttttcttcgg tggtgctttc cgggccgccg gaaagaataa tgccgcttgg
     2581 attgaagtca cgaatttgtg cttctgtcac atcccacgcc cacagttcgc agtaaacacc
     2641 cagctcacgc acgcggcgcg caaccagttg agtgtactga gaaccgaagt ccagaatgag
     2701 gatgcgatgc ttatgaatgt tttccgtcat tgacgcttat tccgaggcaa gtgaaacaga
     2761 taatataaat cgcccgacat gaagtcgggc gaagagaatc aggagcccag acggtagttc
     2821 ggggactctt tagtaatggt cacgtcgtga acgtggcttt cctgaatgcc cgcaccgctg
     2881 atacgtacaa actccgcttt agtacgcagt tcgtcgatag taccacagcc ggtcagaccc
     2941 atacaggagc gcaggccgcc catctgctgg tgaatgatct ctttcaggcg acctttataa
     3001 gctacgcgac cttcgatacc ttccggcacc agtttgtcgg cagcgttatc gctctggaaa
     3061 taacggtcag aggaaccttt ggacatcgcg cccagggaac ccataccacg gtaagatttg
     3121 taagaacggc cctggtagag ttcgatttca cccggagatt cttcagtacc cgccagcatg
     3181 gaacctacca tcaccgcgct tgcgccagct gcgatagctt tggcgatgtc gccggagaag
     3241 cgaataccac catctgcgat aaccggaata ccggtgcctt ccagggcttc aactgcgtca
     3301 gcaacggcag tgatctgcgg aacacctacg ccagtaacga tacgagtcgt acagatagaa
     3361 ccagggccga taccgacttt aaccgcgctg caaccagctt ctgccaaagc gcgtgcacct
     3421 gcagctgttg ccacgttgcc gccgatgatt tgcagatccg gatatttagc acgggtttca
     3481 cggatacgct gcaaaacgcc ttctgagtga ccgtgggagg agtcgatcag cagaacgtca
     3541 acgccagcgg caaccagcgc gtcaacacgc tcttcgttac ccgcacctgc gccaactgca
     3601 gcaccaacac gcagacggcc ttgctcgtct ttacaggcgt tcggtttacg ttccgctttc
     3661 tggaagtctt tcacggtgat catgccgatc aggtggaatt cgtcatcaac caccagcgct
     3721 ttttcaacgc gtttttcgtg catttttgcc agcaccactt cacgggcttc accttcacgc
     3781 acggtgacca gacgctcttt cggcgtcatg taaacgctaa ccggctggtt caggtcggta
     3841 acaaaacgca cgtcacgacc ggtgataata cccaccagtt cgttttcttc ggtaacgacc
     3901 ggatagcccg caaaaccgtt acgctcggtc agttctttca cttcgcgcag cgtcgtggtt
     3961 ggcagaacag tctgcggatc agtcaccaca ccagattcgt gttttttcac acgacgaact
     4021 tcttctgcct ggcgttcaat ggacatgttt ttgtggataa agccgatacc gccttcctga
     4081 gccagagcaa tagccaggcg cgcttccgtt acggtatcca ttgctgcgga aagcataggg
     4141 atattcagac gaatagtttt cgtcagctgg gtgctgaggt cagcagtatt cggcagaacg
     4201 gtagaatgag caggaacgag gagaacgtcg tcaaacgtca gagcttcttt agcgatacgt
     4261 agcatgggca atatctcgac cagagtggtt aataaatatt gccgcggcat tatacagagc
     4321 gtaaccgatt gcatctaccc ctttttgcaa aaaatgcttg ctatccccga agggcgggtt
     4381 actatcgact gaataacctg ctgatttaga atttgatctc gctcacatgt taccttctca
     4441 atcccctgca atttttaccg ttagtcgcct gaatcaaacg gttcgtctgc tgcttgagca
     4501 tgagatggga caggtttgga tcagcggcga aatctctaat ttcacacaac cggcttccgg
     4561 tcactggtac tttacactca aagacgacac cgcccaggta cgctgcgcga tgttccgcaa
     4621 cagcaaccgt cgggtgacct tccgtccaca gcatgggcaa caagttttag ttcgcgccaa
     4681 tattacgctc tacgagccgc gcggcgacta ccagataatc gttgagagta tgcagccggc
     4741 cggtgaaggg ctgctgcaac agaagtacga acagctcaaa gcgaagttgc aggctgaagg
     4801 attgttcgat ctgcaataca aaaaaccact tccctcccct gcgcattgcg ttggtgtgat
     4861 cacctcaaaa accggtgctg cgctacatga tattttgcat gtgttaaaac gtcgcgatcc
     4921 ttctctaccg gtgatcatct accccaccgc cgttcagggc gatgacgcgc cggggcaaac
     4981 tgttcgcgcc attgaactgg cgaatcagcg caatgagtgc gacgtgttga tcgttgggcg
     5041 cggcggcggt tcgctggaag atttatggag ttttaacgac gaacgcgtag cgcgggcgat
     5101 ttttgccagc cgcattccgg tcgtcagcgc cgtcgggcat gagacggatg tgaccattgc
     5161 cgattttgtt gccgatctgc gtgcgccaac gccgtctgcc gccgctgaag tagtgagccg
     5221 taatcagcaa gagttactgc gccaggtgca atcgacccgt caacggctgg agatggcgat
     5281 ggattattat ctcgccaacc gcacgcgtcg ctttacgcag atccatcacc gattacagca
     5341 acagcatccg cagctccggc tggcacgcca gcaaaccatg cttgagcgcc tgcaaaagcg
     5401 gatgagcttt gcgctggaaa atcaacttaa gcgtaccggg caacagcagc agcggttaac
     5461 acagcggctg aatcagcaaa atccacagcc gaagattcat cgcgcgcaaa cgcgcattca
     5521 gcaactggaa tatcgtttag cagaaaccct gcgcgtacag cttagcgcca cgcgtgaacg
     5581 tttcggtaat gcagtaacgc acctcgaagc cgtaagccca ctgtcaacgc tggcgcgtgg
     5641 atacagcgtt actactgcta ctgacggcaa tgtactgaaa aaagtgaagc aagttaaagc
     5701 gggtgaaatg ctaaccacac gtctggaaga cggctggata gaaagtgaag taaaaaacat
     5761 ccagccggta aaaaaatcgc gtaaaaaggt gcattaagcc agcacgaatt caacgcgttt
     5821 tttcgatatc aagccatgcc cgtgctgaca gaagtaatct actgcaccgc aggcttttaa
     5881 cacctgcaac ggttgatggc agtctggaca aagagctttc atttcaataa tttccccgca
     5941 ggatgggcaa cgcgcatggt cgttatcctg atcaagcaca tgctgacatt gtggacaatg
     6001 cagttccata tgaattcctc cggtagccat ccctctacat tcatagaggg aatggcagat
     6061 aaaatactta cggataatta tttatttttc ttgatgtgct tcatcagacg cttacgttta
     6121 cgcatctggg ttggcgtcag ggtgttacgc ttgttcgcat acgggttttc cccttctttg
     6181 aactgaatac gaatcggcga tcccattacg tccagcgatt tgcggaagta gttcatcaag
     6241 tagcgcttgt aggaatcagg caggtctttc acctgattac cgtgaatcac cacaatcggc
     6301 gggttataac caccagcgtg ggcatatttc agcttcacac gacgaccgcg taccagcggc
     6361 ggttggtgat cttcaacagc catcgtcatg atgcgcgtca gcatagaggt ccccacacga
     6421 cgggtggagc tgtcatacgc ttcacgtact gattcaaaca agttaccaac accactgccg
     6481 tgcaaggcag agataaagtg cacacgagca aaatcgataa agcccagacg gaagtccagc
     6541 gtttctttca cctgctcttt cacttcctgg ctcaggccat cccacttatt caccacaatg
     6601 acaagtgagc gcccactatt gagaataaag cccagcagcg agagatcctg atcggaaata
     6661 ccttcgcgcg catcaatcac taacatcacc acgttggcat cttcaatggc ctgcaacgtt
     6721 ttgattacgg agaatttctc tacagcatcg gtgattttgc cgcgtttacg tacgccagcg
     6781 gtgtcaatga gcacatactc acgtccatcg cgttccattg gaatgtagat actgtcacgc
     6841 gtcgtgccag gcatgtcata aacaacaacg cgctcttcac caagaatacg gttagtgagt
     6901 gtagacttac ctacgttcgg acgacccaca atcgccagtt tgatcggcag actttgcggg
     6961 tcgaagtcgt cttcctcttc ttcttcgccg ttctcttccg cttcaaattg cgcccagtat
     7021 tcagcgtctt cgtcgacttc ctcttgcggt gcgagatctt ccatccacgg cagcagcaca
     7081 tgctccagca gacttaatac gccacgaccg tgagacgcgg cgatcgggta gatttcacct
     7141 aaaccaagcg agtagaaatc aaccactgcc tgatcgggat ccagaccgtc agttttgttt
     7201 gccaccagga aggtcggttt ttcacgggag cgcagatgtt tggcaatcgc ttcatctgcc
     7261 ggcatcaggc ccgcgcgcgc atccaccata aacagtacga cgtccgcttc ttcaatcgcc
     7321 agcagcgact gttccgccat gcgggtttct acaccgtctt ctgtgccatc aatcccgccg
     7381 gtatcaatac agataaactc acggccttca atttccgcac gaccgtactt acggtcacga
     7441 gtcagacccg ggaaatccgc aaccagcgca tctcgggtgc gagttagacg gttaaataac
     7501 gtggattttc ctacgttagg gcgcccgaca agcgcgacca caggtaccat gtttaaagcc
     7561 tcatttttat aaatcatcag acaacgcacg ctatattcgc gtcgttgtta aaaacaggaa
     7621 aacggcccct gtccaggagc cgttttcaaa gtgaacgaca gagacgatta acgtgtaata
     7681 gagtacacgg ttccgtcttt tgcctggatc agcagtttgc cgtcagcggc aaccggttca
     7741 gtctggaaac cggaactatc aactttttgc tgggcaacga aacgaccatc ttcgacgtta
     7801 atccagtgca gataaccttc actgtcacct accaccaggt tgccattata cagcaccgga
     7861 gaagtcagca ggcgatgcaa cagatcgctt tgtgtccaca gcgtaacgcc gccatcaatg
     7921 gtcaacgcca tcacccggtc attttgatcg accagataga tgcgattgcc gtcgacgatg
     7981 aaatcattca ccgaacccag ttcgcgtttc cacataatct gaccactgcg cagatcaagc
     8041 gccgtcaggt taccattata ggccagcgcg aaaacaacgc cgttaacaac gacgggagtc
     8101 gtgtcaacat cgctcagacg gtcaatttcg gtagaaccgg tcgcctggga aatacgctgc
     8161 tgccaaatca tctggccctg ttccatcagc actgcgctga cgcgaccatt atcgccaccc
     8221 acgacggccg caccaaaagc cgttgccggc gcagactcgc cacgcaaaga gagcgaaggc
     8281 atatcgaggt taactgtcca tttgacagcg ccgtcagcct cgttcagcgc ttgtaactga
     8341 ccgttactgg tgtggattaa caccagaccg tcgctgacca ccgggcgcga aagtgcttca
     8401 cccgcgactt tagtttgcca tgccacagta ccatcgctgg tattcagcgc gtaaacctgc
     8461 gccttttcgc tgccaatgta gacatgccca ccagacacgg tcagaccgcc agaaagtaat
     8521 gcaggctctt tagagaacca gccatctttc tcggccaggc tgacagacca gatttctttg
     8581 ccatcatccg cattcagcgc ttttaccaaa ccagcgcggt ccgctgcata gacaacgttg
     8641 tccgccagtg ccggatgaag attggaatag aagttgccaa tgccgctacc aacggaagtg
     8701 ctccacgccg tggtcggcgt aaactggttt tcaacggttg gcaatgggga catctttacc
     8761 acatcttctt cgctgttaaa cagcgaacag ccgcttaaaa gggtaacgga aagcagtcct
     8821 ggcagcagta atttacgcaa ttgcatcggg tccctctcag atggacaaat tattaatttt
     8881 catctgcatc atttcgctca gtgccggagt aacatcgctt ttcacgcctg cttcccatgc
     8941 actacgcgca ccttgcttat cacctttgct cagcaatgct tcaccacgca ggtcggcaac
     9001 aatggcagcc cacccttcac ctttgatggt atcaagggtt ttcagcgcgg catcagcctg
     9061 cttgagctgt acctgaacgc gagcaagacg cagatttatc acggctttga gattttcatc
     9121 gctcgtgtct gccagcccct gttgtaactg ggcggcagct ttctctagtt catttttgtc
     9181 aacaaattgc tgcgcaagtt ccaaagaagc cagcgcacca taagtatttt tattttcagc
     9241 agcaaatttt tccgccgccg ggatgctatc cggtttgcct tcgctcactg cggtaaccgc
     9301 attttgatag gcaagagaag cggagcgtgc agaatcaacc tgatggctgt tccagtagcg
     9361 ccagccaatc agtgcgccaa cgcccaaaat caccccaaca gccagtgctt tgccattttc
     9421 agcaaaaaag cgtttaaccg cttctacctg gtcgttttcg ttctcgtaaa tttccacgct
     9481 gtccttctcc ttccttaacc cagtaacgtg cgcaaatgcg cggctacgct atcctgcgca
     9541 actgccgttt gctcaccaga gcgcaaatcc ttcactactg ctgtgccgtt agccacttca
     9601 gactcaccca gcaccacagc aacgcgggca ccccatttat cagcacgggc aaactgtttc
     9661 ttaaagttgc cgccgccgtg gttggtcatc aatttcacgc ccggtaattc atcacgcaga
     9721 cgctcagcta atgccatagc cgcagattgt gtatcagcac ctgaagccac caggtatata
     9781 tcgacaacag gatcggcttt aaattccgga ttaacggcct gtactaacaa tacaagacgt
     9841 tcgaggccca tcgcaaaacc gacagccggt gttgcacgac cgcccagttg ttccacaaga
     9901 ccgtcataac gaccgcctgc acacacggtg ccctgggagc cgagactgtt agtcacccac
     9961 tcgaaaacgg tacggttata gtaatccaga ccacgcacca gacgctggtt tacggtgtaa
    10021 gcgatccccg cgctctcaag cagtttgcac agaccggcaa aatgctcacg agattcctcg
    10081 tccagataat cacctaatgc cggagcgtcg ttgagaagcg cctgcacttc cggatttttt
    10141 gaatccagca cgcgcagcgg gttagtgtac atgcggcgtt tgcagtcttc gtccagcttt
    10201 tctttatgct gctcaaggaa tgccaccagc gcatcgcggt aattggcgcg tgcttccagc
    10261 gaaccgatag aattcagctc aagagttacg tgttcggaaa tacccagcgc gcgccaccag
    10321 cgggcggtga gcataatcag ttcagcgtcg atatccggac cttgcagacc gaaaacttcg
    10381 cagcccaact gatggaactg acgataacgc cctttctgcg gacgctcgtg acggaacatc
    10441 ggcccgatat accacagacg ctgttcctga ttgtacagaa gaccatgctc gatgccggcg
    10501 cgtacacagc ccgccgtccc ttcagggcgc agagtcaggc tgtcgccatt gcgatcctca
    10561 aaggtgtaca tctctttttc aaccacgtcg gtgacttcac caatcgcacg tttgaatagc
    10621 ggggtctgct ctacaatcgg caagcggatt tcactgtaac cgtagctgcc gagcacgttt
    10681 ttcagtgtgc cttcaatgcg ctgccagatg gccgtttcgc caggcaggta atcgttcatg
    10741 ccgcgaatgg cttgaatgtt ttttgccacg tttattctct ttctgaatat aaaaatgaac
    10801 cctcaacgct tccctcaatg tttcgggagc tatgcgggtt caatcataca cgggaagcga
    10861 ggcgcttccc atcacgttat tatttttcaa cctgctgaac gtcaattcga cgcgcttcgt
    10921 ccagctgact ggctttcgca cgaatgcgtg cttccagctg gtcgatcata tcgttgttgt
    10981 ccagacggtc tttgcgcacg ccatcttcat agaggccgct tttcttgttg ccgccggtga
    11041 cgccgagtgt agaaaccagc gcctcacctg ggccattcac cacgcagccg ataatcgaaa
    11101 cgtccatcgg agtgatgata tcttccaggc gttgctccag cgcgttaacc gtaccgataa
    11161 catcaaattc ctgacgcgaa caggtcgggc aggcgatgaa gttgatcccg cgcgaacgga
    11221 tacgcagcga tttcaaaata tcgaaaccga ctttgatctc ttcgaccgga tcggccgcca
    11281 gtgatacgcg cagcgtgtcg ccgatgcctt cagacagcag cagacctaaa ccaatggcgg
    11341 attttactgc cccgctgcgt gcaccaccgg cttcggtgat ccccagatgc agcggctgat
    11401 cgatctgttt tgccagcaaa cgataagact caacagcgag gaagacgtca gacgctttca
    11461 cgctgacttt gaactgatcg aagttcaggc gatcgagatg atcaacatgg cgcatggcgg
    11521 attccagcaa cgcctgcggc gtcggttcgc catacttttc ttgcagatct ttttccagcg
    11581 atccggcgtt aacgccaata cggatcggaa tgtttttatc gcgcgcacag tcaaccacca
    11641 tgcgaatacg ctcttcatta ccgatattgc cagggttaat acgcagacaa tcgacgccgt
    11701 attccgctac tttcagtgca atgcgatagt cgaagtggat gtcagccacc agcggcacgt
    11761 taacctgctg tttgatgagt ttgaacgctt ctgccgcgtc catcgtcggt acggagacac
    11821 ggacgatatc agcgccaacg cgttccagcg ccttgatttg attgaccgtt gcttcgacgt
    11881 ctgtcgtacg cgtattggtc atggactgta cggcgatggg agcaccatcg ccaatcggca
    11941 cattcccaac gtaaatacgt gttgattttc tacgttgaat tggagcctgg ttatgcatga
    12001 aaaatctccc gcgttacccg tctgttactg cgccggtgat tgttcggcat tgagggtcag
    12061 acgcgcaacc tggttagttc tgataaaacg actcagatcg acaggtttcc cttgatactg
    12121 gatctgtact gcggctggcg caccaatttt cagtttgtat ggtgcctggc cggttaagtt
    12181 caaattaccg tctttacgct gcataccgct aaacaatttt ttaccggtag catcagtaac
    12241 ctccagccag caatcggcag taaagttcat caccagcgca ttcggatcag ccgccggcgt
    12301 agtcacgcct gcctgatcgg ttggcaacgg cgcagcgcca tctggcgttg ttgttgccgt
    12361 tggtgccggg gtcgcggcgg tatcaacatt tgcctgcgaa ggcgaaacaa ccgcattctg
    12421 ttgcggatca acagctggtg ctggcgcagt tacggcaggt gtttgcgtgt tggttgcggt
    12481 agtatccaca gacgctggcg gcgtggacgt tgtagccggg tctgtagttg tcgacgtatt
    12541 taacggaaca ctctgcccct gctcgctatt actgctcagt tccgccgaag attgatcggc
    12601 catagtggtg atctcttcct gctgagcttt gtggtcttgc caccaccagg caccgctcag
    12661 gccgataacc acaaacaaca ccagccaggt gaaggtcatc agccagccgt cacgtttttt
    12721 gcggcgttta ccgagggaaa aactctgcat aggcgcaact tttgcagccc gtagtggagc
    12781 ctgcttttcc agccctggca gcagttcttc ttctggaata tgtaccagac gcgcataaga
    12841 gcggatatat ccgcgcagga atgttgaagc aagatcggcg ggtgccttat cttcttcaat
    12901 gtcgcgtacc gtggaaacct tcaggcaaag tcgctcggca acggcctgct gactaagtcc
    12961 tagttgttcg cgagcattac gcaggcgagc gccggtagta agtgcttcat tttggtcgtg
    13021 cgtggcttca gtattcattc gctgcaggta cgtttaaatg agaattgaga tgccggtgaa
    13081 ccatcatgct cacccacacc gcgaaacatc cgttaagtta accgttatca tacagtataa
    13141 gaccgtctgt gtgctcgtga caaagcccac attcactaag gctaacttac tgttgcatcg
    13201 ttacatactg ccttaaagtc agcaaaaacg caccgttaat attgaccaga caattgcaac
    13261 ttaatacttc attcataaaa gattacgcca cggtacataa agtaaccgtg gcgtaatggc
    13321 tatcagaccg ctttaatgtc gatggcttca ccctgcatcc gtttacgcag ggtacgtttc
    13381 gtacggtcga taacatcgcc cgccaactga ccacaggcag catcgatatc atcaccacgg
    13441 gttttacgca caatagtggt gaaaccgtag ctcatcagca cttttgagaa acggtcgata
    13501 cggctgttcg agctgcgtcc atacggcgcg cccgggaagg ggttccacgg gatcaggttg
    13561 atcttacacg gcgtatcttt cagcagttcc gccagttggt gcgcgtgttc agtgccgtcg
    13621 ttaacgtggt caagcatcac atactcaata gtgacacggc cctgattggc gttggatttc
    13681 tccagataac ggcgcaccgc agcaaggaac gtttcgatat tgtacttttt gttgatcggc
    13741 acaatttcgt cacgaatttc gtcgttcggc gcgtgcaggg aaattgccag tgcaacgtcg
    13801 atcatatcgc ccagtttatc cagcgccgga actacaccgg aagtagaaag cgtgacgcga
    13861 cgtttagaca ggccaaaacc gaaatcatca agcatgattt ccatcgccgg aacgacgttg
    13921 ttcaggttga gcagcggctc gcccatgccc atcatcacta cgttagtgat tgggcgctga
    13981 ccggtgactt ttgctgcgcc gacgattttc gccgcacgcc acacctggcc aataatttcc
    14041 gatacccgca ggttgcggtt aaagccctgc tgggcggtgg aacagaattt acactccagc
    14101 gcacacccca cctgcgaaga gacgcagagc gtggcacggt cgtcttccgg gatatacacc
    14161 gtttcgacgc gctgatcgcc aacggcgatc gcccatttaa tggtgccgtc ggatgaacgc
    14221 tgttcttcaa ccacttccgg tgcgcggatt tccgccacct ctttcagttt gccgcgcaac
    14281 actttgttga tgtcggtcat ctcatcaaag ttgtcgcagc aatagtgata catccacttc
    14341 atcacctgat cggcgcggaa gggtttttca cctaaatctt taaaaaactc ccgcatctgc
    14401 tgacggttga gatccagcag gttgattttt ccatctttcg tggtgacgtt ttcaggtgtg
    14461 actaattgtt cagacatatg ctattccggc ctcgttatta cacgttatgg cccctggagg
    14521 attgaaaaaa gaaacgcccc ggtgagcggc tgctcgtccg ggggcgctgc attgtacaaa
    14581 ttctggcgca cggatgccac gtttgcacgc ggcatttacg aaattattaa cgggtgcgcg
    14641 ggcacacttc gccttcgcca aagaaataag cgatttcgcg agcggcagat tcgacggaat
    14701 cagaaccgtg ggtaccgttt tcggtcaggc tgtcagcgta atcagcgcgc agagtaccag
    14761 ccagtgcgtt tgccggattg gtcgcgccca gcagatcgcg gtgacgctga acggcgtttt
    14821 caccttccag cacggaaacc acgatcgggc cagaggtcat gaattcaacc agaccatcaa
    14881 agaacggttt tccatcgtgt tcagcataaa agccacgtgc ctgttcaacg gtcaggtgca
    14941 gcattttggt gccgacaatt ttgaaccctg cagcttcaaa gcgcgcaaag atattaccaa
    15001 tgacgttttt tgctaccgcg ttcggtttga tgatggaaaa agtacgttca atagccattt
    15061 ttacctctgt aaattgttct gttgttgtct gtaccagcgt acgagatggc gcggattata
    15121 atgagcaaca gggccgttga ctattgatga aggtaacatt tttttaaaat aaaatgagtt
    15181 ttagcaacaa tcagggacaa aacagactat tgcatgacaa atttcactgt cgcgatttgc
    15241 cccacatcat ccatcaccag caattgataa tcacctttat ccgtcaaatg cagggtcacg
    15301 ttgcgcccgc gttcagttaa cggttcgcca ttcagaaacc accagcgttc acctgcccct
    15361 ccacttgatt gcaacggcaa agtcgcttcc gctgcgcccg gtaaacgttt aataatcgcg
    15421 ccatcgcgaa cgcctgtcag ctgcagaggg agctgggcat cgtgaccgta tgacgggcaa
    15481 attgtcgagg ccggtggtaa gcgcacagcg cgacgttctg atgcgggcag ccagggttcc
    15541 agcggtagcg gccagacatt tatcatttct tgtcgcgctt gcgggcaatc agcggcaaca
    15601 cgtttgccat tttcatccag ccagatgggg aaacgaatgc cattaatgcc ttcctgctcc
    15661 ggcagtaata gagttggcgg ctgactcccg tccagcagcc aggttgccag gcggcggcga
    15721 cagttaccgt caccttccgg caaagactgt ccgcccggcc agcagataac gccacgagtg
    15781 actgagttcg ggcgcgggtc ttccggcaga ttcgcactgc gcgacagtaa gatattattg
    15841 acctgattca acaatggtac ggcactggca aagccaaact gaccaacaac gggcgtgccg
    15901 tccggtctgc cagtccagat cccaatgaca tagcgagcgt taaccccaat cgcccaggcg
    15961 tcacgatagc catagctggt gcccgttttc catgccagtg gggcgacgcg cggcaaggca
    16021 ctatccggca agggtttcgc ttcatcagcc ataatccggc gaatgatcca cgccgccccc
    16081 gacgacatta aaggccgttc aagcagcgga tcgtcaggct gtaagcgcaa tttgcctgcc
    16141 ttgccgtggc gagcaaacgc ggtatacgct gccgccatat cttccagttt tgcaccagcg
    16201 ccgccgagaa tgagtgaaag attcggcgca gcaccgttgg gcaaatataa cggcaatcca
    16261 acattgcgta actttgccgc aaaccgtttc ggtccatagg cttccagcac ctgcacagca
    16321 ggtaagttca gcgagcgcac cagcgcctcg ctcatgctga tcgggccatg aaaaccgcta
    16381 tcaaagttac ctggtcgata atcaccggtg cgccggggga cgtcttgcag cagtgatgcc
    16441 gggtggatca agccttcatc cagcgccaga ccataaacaa acggtttgag caccgatcct
    16501 ggcgatcggt tcgcattgac catatcaaca tgaccaaagc gtgaatcatc gttgagatca
    16561 accgatccca cccagccgcg aacacgcata tcggtatgat caaccacgat catcgccagt
    16621 gagctgcgcg gtggcaaccg ccctttccag ttttgcgcca gttcttccag acgtcgttga
    16681 agaccggcat ccagcgtagt aacgattttg tcgcttttgc ttttaccgag catcatgcgc
    16741 gaaaacagcg gtgccagttg cggcatttgt cggggtgcca gccagatggg ttcttccctt
    16801 gactctttta cctgctcacg ggaccacgca ccttgcacgg ccatccgttc gagcacttta
    16861 ttacgcgcgg cttcggcacg ctccggccaa cgatccgggc gaagacggct gggcgcttgc
    16921 ggcaaaaccg ccagcattgc cgcctcggaa tagcttaaat tcgcaggcga ctttccgaga
    16981 taagcccaac ttgccgcacc gatcccctgc aacgtaccgc caaacggagc gcggttaaga
    17041 tacaaggtca gaatttcacg cttagacaga tgccattcca gttgcaacgc gcgccagagc
    17101 tggcgaattt tgccgccaaa tgttttgggg tgaggatcaa gcagacgagc aacctgcata
    17161 gtgagcgtgc tgccaccgga aataacccgt cccgaagtga gatcttgcca tgctgcgcgc
    17221 gccaccgaga atggattcac ccccggatgc ttccagaacc agcgatcttc ataattgatc
    17281 agcgcttcaa ggtaacgtgg agaaacatct tcgattgtta ccggataacg ccagatgccg
    17341 tcagcatcgg cgaagcgcca tagcggcgta ccatcctgcg ccacgaccac tcgtgcggga
    17401 ttgacttcat gcagcggtag aggccagagt ttatctgctc cccacgctgc aagaaaaagg
    17461 agaaatggcg cagctgccag cgttatccag cagccgcgtt tggttaacaa gcgaggcatt
    17521 tacggtcgga caatcagcag atcttcagcc gcgccggtcg cccgccattg gggaacatac
    17581 attgattcca ccatcggttg cggtacctga tacgttcccg gcgtcaccgc ccgcgccaga
    17641 tacaccagcg ttaccggttg gtattcatca acggcaaccg ccgccacaaa gcgatcgtca
    17701 cggaactcaa tgtgcttaat actcgcctgc tgcatctggt tcagtaagtt ctgcacttcg
    17761 ccaccacttt gctccaggct ggcgctatcg ttcgccagat tctggttttc cagttccaga
    17821 cccgcaggca gcagatccac gaccaacgca tccggcacgc tgttactggc ttttacctgc
    17881 aaccacacca gcaccagatc gccgctacgt aacgagtcca gcgatttgct cttaccatca
    17941 gtaccaagaa tatgacgctc gatttgcagc acatcgttcg ccggtaaagg tgcggattgc
    18001 ggataaccgc tggcatccac acgcagccat aacggctgat cgccactgtt tctcacctgc
    18061 aaggtggcaa gttgatcgct attcagattg ctgtttagcg ttttctcgcc tgtcagcggc
    18121 tcagctgaga aagaggtttg cgcctgccat ttacccggta aatcctgaat cgtacgggca
    18181 gccaggaaca acgcgttact ttcctgcgtc gatagccagc gttcaccaaa tgcctgctgc
    18241 gaaagcgtgt tcagcaaagt gttctgctca tcgggtagca gtttattttc ttccagcaag
    18301 gagagcatta acgcgttgtc gcgcagtgga ctaccgtaat cacccagcca tatccgctca
    18361 tcactatttc tcggcgtttt cagcgccagc acaatcgctt cttcaccacg cgttgcatca
    18421 cccatggttt tcagcgcaac gccaagttgc agcagcggta aaccagaagc ggcatctgca
    18481 cgatgctccc agatttcacg cagcgcaccc agtggtgctt tttgctgacg ggccagcacc
    18541 agcgcagcgt aagactgaac ggcgaattta ctggctttga gattatccgc gtacgggatc
    18601 gacatcatgc ccggatcttg taaatagcgc agcagacgct cattaccccg gttaatggca
    18661 tctgtcggca cgctgtaacc ctgttcgcct gcgcggacca ggaaatccat cacgtaagcc
    18721 gtcagccagt actcttcgtc accgttttta tcccacagcg caaagccgcc gttatcacgt
    18781 tgcatttgca gcaaacggga aatgccgata tcgaccgatg cacggcgttt ctcatcactg
    18841 tcgcctttga tgcccaacgc ctgcagttgg gctgcgttgg tataaagtga cgggaacagg
    18901 ccgctggcgg tttgctcaag acagccgtac ggatacgctt ttaactcttt gatataacgt
    18961 gcaatgttca gcggtggttt gccgctcaac aacaattgcc cttccagcgt aacaggcgag
    19021 aagttttgca atccatccgc cggaatcgcc catgtctcac caggctgtaa cgccgtaccg
    19081 taattaaccg tttgtgccgg gaacgccgga cggacgccga ttttccactg cttatgctga
    19141 tcggcaacgg tttcacccgg taacgctaac ccgctaatgg tggcctgaat ttctccatcg
    19201 ccataacccg gcaatgctcg caccgggata aacagcgtag tacgcacacc tggcgctaat
    19261 tcaacggctg cgggtgaatc gctgaccagt tcaagcaaac cactggcggt cagggcaacg
    19321 ttcagttttt gcggtttatc ggtaagatta gtgatatcca gcgtcagacg cgaggtatcg
    19381 ccactcgcca taaagcgcgg catgttcagt tcagcaatca ccggtgcggc aactatcact
    19441 ttgctttcgt tgctaccgaa atcatctgcc gtccaggctt gcgccatgac gcgcaattca
    19501 ccgttaaaat cgccaatcgg cagtgtaacc gagccttcgc cctgttcgtt gagcgttacc
    19561 ggcagcgcct gctgcgcgac aatattgacg tgattgaccg gcggtttacc accacgtttc
    19621 agctcatcac catcgccacc gaaacgcaga gctgccagac gcccctgacc ttcaataacc
    19681 tgaccgtaaa tatcgtaaat gtctgcgcca tagcgtttct gaccaaagaa cgcctgccac
    19741 ggatctggcg tgacgtagtc agtaatattc agcacaccac tatcaacggc agacaccagc
    19801 acattcacct gtttaggctt ctcgccattt ttagtgctgg ctttaatttt cacggttaat
    19861 ggttgattgg gacgcatttt tgctggtgtt tccagcgcca gatcgaggcg acggttttca
    19921 tcgccaagcg gcagatgcaa cacaccaacc gcgcgttttg gcgtcgcgga gcgagattta
    19981 tcgccaggac gtaccaccag cgtacttaaa tacagatcat gacgattcca ggttttatcg
    20041 accggaatcg tcagatccag cccttgagcc cgaacatcaa tctcttgcca ccacagcggc
    20101 ccttcactgg actcgaccat cgcataacct ttacccgccg ttggcgcggc gatatgcaac
    20161 ttaatggtgt cgccagggcg ataactggct ttatccagtt tcagcgtgac acggtcgggt
    20221 cgcactgcgc cgctaccgtc gctgttgtcc tgccagctat agccagccca gaaacgaaca
    20281 ctactgaccg cttcattcgg cgctttgact tccagacgat aagcgcccca ctctaccgga
    20341 aaactgacct tgccggtttc gtccgctttc agatccagag tttgttcatt ttcgatcaga
    20401 tctttttgat caaactgtga ctgccagcct tcatcttctg accagttcca gtagtaatcg
    20461 cggcgttcgc gaatcaggcg cacctgcaag cccgacacgg cttttttcac gccttgcgca
    20521 tcgctataaa cgatgtcaaa agcggcgtta ctgccttcat caacaatggg ctgtttcacc
    20581 gtgctgtcag tacgataatc gtaaaccgat ttcgaggcga actgcggacg gatccccggc
    20641 aatgcatcgg caggccagat agcctgctca gcgcggcgcg tcaccgggcg accgcccgat
    20701 tccagcaaac taccctggaa aataacctgt aatggggaat gcgtttcctt ccactggctt
    20761 tctgtagaaa cttcgccgcg ccctttatca tccagcgtca actgaacttc atccagcgtg
    20821 cgggaaagat tttcggcagc tatatcgccg aattcaaaac caggtaaggc tgacacagct
    20881 tcacgcagtg ggcgcaggaa aagttgccct tgcaaagtat taccattagc aggtgcacca
    20941 tacaggtagt accccaccac ggagaatttc acttcatctt tcggcgttag cggggttttc
    21001 tcaccggtca gattcagcgc catgcgctct ggcataaaat cttcgacgtg gaaatcccac
    21061 atccgatact ggttatcgcc cgtgttagcg cgaatatgcc acataccggt tgccgcattg
    21121 ctatcgagtg gccaggtaaa gtggtagagg ccattctccg gctgactaac gacgctcctg
    21181 agtacctgcc catcgggttt aatcacgtct aacttgatgg gttgattggg caacgcttta
    21241 ccgtctgcat cacgcagcaa accattgagg attaccgttt cacttgggcg ataaagatcg
    21301 cgtgggccaa acatgaaaaa ctgtttgcta tagcctggcg cgccagcaat gttaaattct
    21361 gctaagtcca gcgccggaag ttttaaatcg agtagcgttg tctgaccgtc tttacgcgcc
    21421 aacagtaatg ccgcgttttt atcattttcc agctgcacat gcccctgagc gtcactggtt
    21481 gcctgagtca gagtctgccc tttctcattt aataaagaga cttcaattcc ttgctgggcc
    21541 gcgccgtttt ccagactttg ggtaaagata tccagacgat tgtgataacg gtgagctgaa
    21601 acgccgatat cacttaacgt aaacagcgtc gcgggattac tgtaatcgta acgtccagcc
    21661 tgattcatca cagccagata cacgcccgcc tgctgaaggc ggtttgatat cgcccagcgg
    21721 cagcaataat ttttcacggg tgttacgcgc aggattgaga tcaaaccgtc cggtgtagac
    21781 cagatccgcc atctgcagca gtttgtctga ctgccagttc gccagcgaat tgcggtattc
    21841 ccattggcta atgaatgctg gcagagattc tggcttaacg cggaagaagt taacatcgac
    21901 attattaacg ttgagcgcca ttaccggcag cccttcaacg actttgccag gcagcagcga
    21961 accacggctg gcaaaaccga cgctgggttg gatgtcgcgg gtagttatag ttttttcgta
    22021 atctttactg aaggttgcgt tgttgagtac tttgacctcc ttgccaatag taacgatcaa
    22081 atcacgtttc ggttcgaggt ggcgtaaacg taactcttta agattatctg acagctccca
    22141 ggcaccatcc actttgccgc tttttttatc gacgacatga ataacgcgtg agaaatcctg
    22201 atccgggtcg agagggatgg agaacgtcag caccagcgtg gctgcaccat caagttggac
    22261 ttcagagaga tccagcaatg tcagcacctt accggcactc tgttgggcca gtttttgtct
    22321 ctccggcacg gagagttttg ctgaactcgc gttttctgaa gaggccgctt tagtaacttc
    22381 agaaggcgca tcttttttca ccgctgttgg cgcgttatcg ttgttgtcgc accctgccag
    22441 cgccagcatt agcatgcagg cggctacgcg taactttttc atttttcatc cctgccacaa
    22501 tggcccgtta gcaacgtcga ataattatta tgcgtgagaa tttacgttat gtaattcagt
    22561 atcacgccca tacaatccga attttagtga gaattgtggc tcagacagcc aaatcagcct
    22621 ctttcgcttg tcgcttttca ctaaacatcc gacaatttag cctaaccccg gcaaaaatgg
    22681 agatgcctat gtccacgaca tggtttgtag gagccgactg gctcgccgaa catattgatg
    22741 acccggaaat tcagattatt gatgcccgca tggcgtcgcc tggacaggaa gatcgtaacg
    22801 ttgctcagga gtatctgaat ggacatattc ccggcgcagt gttttttgat atcgaagcgc
    22861 tttctgatca cacttccccg cttccgcaca tgctgccgcg cccggaaacg ttcgccgtgg
    22921 cgatgcgtga attaggcgtt aaccaggata agcacctgat tgtctatgac gaaggtaatc
    22981 ttttctcagc cccacgagca tggtggatgc tgcgcacctt tggtgtagag aaagtgtcga
    23041 ttctgggggg tggacttgca ggctggcagc gcgatgatct gctgttagaa gaaggtgcag
    23101 tagagctgcc ggaaggagag tttaacgccg cgtttaatcc tgaagccgtg gtgaaagtaa
    23161 ccgatgtatt attggcaagc catgaaaata cggcgcaaat tattgatgcc cgcccggctg
    23221 cacgttttaa cgcagaagtt gatgaacctc gcccaggttt acgtcgcgga catattcccg
    23281 gagcactgaa tgttccgtgg acggaactgg tgcgcgaagg cgaactaaaa acgaccgatg
    23341 aactggatgc gatatttttt ggtcgcggcg tcagctacga caaaccaatt atcgtcagct
    23401 gcggctctgg tgtaacggca gccgtggttt tgttagcact cgcgacgctg gatgtgccaa
    23461 acgtgaaact gtacgacggc gcatggagtg aatggggcgc gcgggcagat ttaccggttg
    23521 agccagtgaa ataagtattt tacaggcaat aaaaaaccgc cgaatttggc ggttttttat
    23581 tgctggtctg gttcgcggcc tttccagcag gttgtattac cgtagtaatg caagcgcgtc
    23641 tcagcggaga caatactcgc cagtaactct ctttttgtca agcaaaagag agttattatt
    23701 gttctgttag tgtattatcc actgcggccc tttccgccgt ctcgcaagcg ggcgctggct
    23761 taaggaaagg atgttccgcg accgttagag caagcgcgtc tcagcgcttg ctatcgcggc
    23821 aatatcgcca gtggtgctgt cgtgatgcgg tcttcgcatg gaccgcacaa tgaagatacg
    23881 gtgcttctgt atcgtactta ttgtttctgg tgcgctgttt tcccaagtaa ataataaccg
    23941 gagtctctcc ggcgacaatt tactggtggt taacaacctt cagagcagca agtaagcccg
    24001 aatgccgccc tttagggcgg catattttag attatccggt tctgtttaaa gtcacgcaaa
    24061 aaaccacccc agcgacgttc atagaatggc gcaatatgtt cggtaataaa gtggctaatt
    24121 cctttttccc cttttttcac ctgacaaata tcgattggtt catcgccagg taatgtatcg
    24181 gtcgctacac ttcccgtcgc ctgaataatt tcttcgatat caccatcggc ttcaatgcca
    24241 ataagtaaat taggctgtgc ctcttcgttc tctttaattg aacaaataaa agcacgcttc
    24301 accggcttaa tggttttaaa taaggtggtg agtgaatcaa tcatttgtgc tggcggctct
    24361 gcgacttccg ataatatcag cgattcaccg ccttccagga tttcctggct gctcagcgga
    24421 tttccctctc caccaatcaa caaactgatt tcacgcggca taaattcttt accggtcggc
    24481 agtttggcat taaggaagag cgtttcgcca agggtcatct caaacaatgt gcgaacgggc
    24541 attacgacaa atgcctgttc gtcttcaacc gcctgttgaa gcgcttctaa cgaggtgaaa
    24601 aaaggaatga cgctggtgcc gtcttctttt tcccagtgct gtaaatcaag cgcactatct
    24661 tcaaccacag cctcgccctg cgccgccgta ccaggcaccc agacggtgga ttccagtaga
    24721 gtacggaaaa agcccgggcg gtgcgccggt tcggttgctg ctttttccag caggtcttca
    24781 agttcgtttt ttgtttcgga catatgaacc acaattcatt caacgttcgg ggcgcaaatg
    24841 cctgatgcgc tacgcttatc aggcctacaa gggattcgca atttgttgaa tttgcagaat
    24901 ttgtaggccg gataaggcgt ttacaccgca tccggcatat tagtttacgc cgttaacaga
    24961 ttagctatcg tgcgcacacc aagtcccgta gcgcccgcag accactgttc aaccggcgct
    25021 ttacggtaag tcgccgagca gtcgatatgc agccagcctt gctgatagtt ctcaacaaag
    25081 tgcgacagga agcccgccgc cgtgctcgcg cctgccggat acgccgcgct tccggtattg
    25141 ttcagttcgg caaagttaga cggcagctgg ctgcggtgga attccgccag cggcagacgc
    25201 cagaatggtt cgttctcttg tgaggcactc gccagcaaac gaccggcaag cgcatcgtca
    25261 aaactgaaca gcgcgtgata atcattaccc agcgcagttt tcgccgcccc ggtgagggtc
    25321 gccgcatcaa tgatcatttc cggtttctgc gcactggcat caatcagacc atcggcaagc
    25381 accagacgcc cttcggcatc agtgttcatc acttcaactt ttttaccgtt gcgataggtg
    25441 atgatatcgc ccagcttgaa cgcattgccg ctaatcaggt tatccgcaca gcagaggaac
    25501 agcttcacgc gcttgttcag tccgcgcgta atggcaaatg ccagcgcccc ggtaaccgtt
    25561 gccgcgccgc ccatgtccga cttcatcgag tccataaacg cagtctgttt gatgctgtag
    25621 ccgccggagt caaaagtgat acctttacct accaggcacg cgtacaccgg tgcttcttta
    25681 tcgccagttg ggttgtaatc cagcgccagc aataccggag aacgttctga accgcgcccg
    25741 acggtgtgca gccccatata accttgctca cgcagatctt cgcctttggt gatccgataa
    25801 gtcacacgat cgcccgcgac gttgctgatc agatcaacag cacgctgtgc cagttgcgat
    25861 ggtcccaatt cttctgccgg tgcgttgatg gtgtcacgca cccagtcgat gatcatcagg
    25921 cggttatcca gttcctggcg ctgggcatcg tccagatccg gccactccac tttacgcgtg
    25981 cctttcgggg ctttgtaacc ttgccagaat gcccagcagc gatccgcatc ccagccttca
    26041 ccgcttaact gaacatgctt gatgcccaga ccgtcaatct tgcgcgccgc acgctggatc
    26101 agccccagat cgtctgcccc gttcaaatgc agggtaatgc cgtcattatt aatgctgtaa
    26161 gttgcttttt ctccccagcg cgcgtcggca ggttgggtag agagggtaat cttcatcgct
    26221 tctgtcattt tagttatcct tctttgtaaa agggccgcca gttggcagcc ccaggatttt
    26281 tccgctaaat gattcgcgtt gcaggaaggc ggcaagtgag tgaagcccca ggagcataga
    26341 taactatgtg actggggtga acgagcgcag ccaacgcatc tgcggcgtga agcatgacgc
    26401 ggaaattatt cggcctcgtc cagccagact aacaaaatcg cttcgaggat tttctcgttg
    26461 gatgcctgcg ggtcgtcgtc gaaatcttcc agatcgcaaa tccactgatg catatcggtg
    26521 aatcgaaccg ttttcggatc aagatcggga tacgcatcgt acagtgcttc gccaatttcg
    26581 cggctatcgg tccatttaag tcccatacta acctctgtta atgctcacgc gcatggttga
    26641 tagtgtaacg cgggatttcg accactaaat cttcgtcggt gacgcgcgcc tggcagctta
    26701 aacggctttc cggctccagt ccccaggctt tgtccagcat gtcgtcttcc tgctctgagc
    26761 tttccggcag cgagtcaaaa ccttcacgaa cgatgcagtg gcaggtggtg caagcacagg
    26821 atttttcaca ggcgtgttca atctcgatac cgttacgcag agctgcgtcg agaatggttt
    26881 caccgctatt agcttccaga acagcgccat cagggcagag atcctgatga ggcaaaataa
    26941 caatctttgg catattaaac ctcgtccacg gaatggcctt tcagcgcacg acgaaccgac
    27001 tggtccatgc ggcgagcggc gaaatcctgg gtttgtttgt ctacgttttt aatcgcttgt
    27061 tcgatggcgt caacgtcatc gccctgcgcc acttcactca ggtgagcggc agcatcgtca
    27121 atgacctgac gttctgcggc gcttaacagc gcggcatcag cagccagcgc gccgtgcaga
    27181 ctttccagca cacgcgccgc ttctactttt tgttctgcca gcattcgggc ttttacgtcc
    27241 tgctcggcat agctcattga gtctttgatc atcgaagcga tttcgctatc ggtcagaccg
    27301 taagacggtt tgacctgaat agacgcctca acgccggtgg atttctccat cgccgtcacg
    27361 ctcaaaagac cgtcggcatc gacctggaac gtcacgcgaa tatgcgcacc gccagccggt
    27421 agcgccggaa taccacgcag cgcaaaacgc gccagtgagc ggcagtcctg caccagttcg
    27481 cgctcaccct gcattacatg gatagacatc gccgtctgac catctttaaa ggtggtgaaa
    27541 tcctgagcgc gggccaccgg aatagtggta ttacgcggaa tcactttctc caccaggccg
    27601 cccatcgttt cgaggcccag cgacagtggg atcacatcaa gcaacagcat ttcgctgtct
    27661 ggcttgttac ccaccagaat atccgcctga atcgccgcgc caatagcgac gactttatcc
    27721 gggtcgatgg aagtcagcgg tggacgaccg aaaaattcgc ctacccgttc acgcaccagc
    27781 ggcacgcgag tagaaccgcc caccatcacc acttccagca cttcgtcagc ttctacaccc
    27841 gcgtctttaa gcgcgcgacg acaagccagt aaggttcgtt ttaccagtgg cgcgatcagt
    27901 tcattgaatt gttcacggct gatttcgccc tgccagcccg caacgttaac ggtcacggag
    27961 tccgcatcgc tcagcgcgat tttggctgca atggcggcat ccagcagttc acgctgaacg
    28021 cggttatcgc tacgatcagg aatgcccgcc tgctcgcgaa tgtaatccgc cagcagatgg
    28081 tcgaaatcat caccaccgag cgcggaatca ccgccggtcg ccagcacttc aaacacaccg
    28141 cgacttaagc gcagaatgga aatatcaaac gtcccgccgc cgaggtcata aacggcgatc
    28201 acgccttcct gaccggaatc cagcccgtag gcgatagccg cagcggtcgg ttcgttaagt
    28261 aagcgcagga cgtgaaggcc cgccagacgc gccgcgtctt tggtgccctg acgctgggca
    28321 tcgtcaaagt acgccggaac ggtgataact acaccatcca gctcgcctgc cagggcttca
    28381 gttgcccgcg ccgccagtgc tttgaggatg tccgcagaaa cgcgcaccgg gttcagcagc
    28441 cccgccgccg tttcaatcat cggcaggccg ttttcgctgg cctggaattg ataaggcaga
    28501 tgcggatagc gttgctggat atcagccagc gagcgcccca tcaggcgttt aacagaacta
    28561 atggtgttgg cggtgtcaag cgctgcgtta gtacgcgcgt cataacccac cgaatgccct
    28621 tgctgttgat agtgaacaac agatggcagc aggtgacggc cttcatggtc ggctaacgtt
    28681 tcggcctgac cgctgcgcac tgtcgccacc agcgagttgg ttgtgcccag gtcaatacca
    28741 gccgccagac gacgctgatg cggcgcagca ctcaaaccag gttcactaat ttgtaataag
    28801 gccatgttta gcttccagaa attaaaaatc gagcagtttt tcttcgagtt gttcggcact
    28861 gctccgcagt ttatcgagaa aacgcagctt acgcacggta tccgccgccg cgtcccacgc
    28921 ctcgttgtct aactgttcaa ccatcaactg atggcgggta tcaaacatct ttttcacacg
    28981 tttgataaag ctttccagcc gcgcttcatc tttcgcctgt tcgatctcgt ccagctcttc
    29041 gcgcagctcc aactgttcca tcaggaacgc ggtgtcgcgc acagtatgct gctcgctggc
    29101 gagatcaaaa ccgtgtaaag aaagcaaata ttccgcgcgc attaacggat gacgcagcgt
    29161 ttgccaggcc tggttaatgg ttgcagattg ctgtacggcg gcgagttgtt ccgcctggct
    29221 tccgctggcg aatttatcag gatgatactg acgttgtaga tcctgaaaac gcaggctcag
    29281 cgcctgggta tcgagttgat aacgggcagg caagccaaag agggtgaagt aatccataac
    29341 attctcaggg ctgcgatccg ccgcgataac ggcgaatcag gtaaaacaaa ccccacgcgc
    29401 aggcgaccac ggtggggtta tcggtatgcg catcaaacgt ggaagctttc gccgcaacca
    29461 cactcatctt taacgttcgg gttggtgaat ttaaaccctt cattcaggcc ttcttttacg
    29521 aagtccagct gcgtaccgtc cagaaattgc aggcttttgc catcgaccac gactttcacg
    29581 cctttgtctt caaacacgat gtcttccggc gtcggttcgt caacaaattc cagtacataa
    29641 gccatacctg aacatccgga ggttctcacg cccagacgca ggccaaaccc tttgccgcgg
    29701 ttagccagaa aggtatttac tcgcgctgct gcactgtcgc tcagtgtaat cgacataacc
    29761 aaacctcaac tcttattttg cttcacgttt gcttttatag tccgcaatgg cggctttgat
    29821 cgcgtcttct gccagaatag aacagtgaat tttcaccggc ggcagttcaa gttcttcagc
    29881 aatatcggta tttttgatcg cctgcgcttc gtcgagagac ttccctttca cccattcggt
    29941 gaccagggag ctggaagcga tagcagaacc gcagccgtaa gttttaaaac gcgcgtcttc
    30001 aatgatacct tcatcgttga ctttaatctg caacttcatc acgtcgccac aggccggtgc
    30061 acccaccatg ccgctgccga cgttctcgtc gttgttgtca aaggaaccca cgttacgcgg
    30121 attctcgtaa tggtcgataa ctttttcgct gtaagccatt ataaattctc ctgattccga
    30181 taccgattaa tgatgagccc attcgatgct gttcagatcc acgccctgct tgtacatttc
    30241 ccacagcgga gaaaggtcac gcagacgacc gatggattta cgaactaact cgatggtgta
    30301 gtcgatctct tcttcagtag taaaacgacc taaagagaaa cggatagagc tatgtgccag
    30361 ctcgtcgttc agccccagcg cgcgcagcac gtaggacggt tcgaggcttg ctgacgtaca
    30421 ggcggaacct gaagaaactg cgaggtcttt cagcgccata atcagcgact caccttcaac
    30481 gtagttgaag ctgacgttga gaatgttcgg cgcaccgtgt tccaggtcac cgttcaggta
    30541 aacttcttcg atatctttga tgccgttcca cagacggtta cgcaggccgc gcagacgttc
    30601 catctcggtc gccatctctt cttttgcgat gcgataggcc tcgcccattc cgacgatctg
    30661 gtgaacaggc agagtgccgg aacgcatacc gcgctcgtga ccgccgccgt gcatttgcgc
    30721 ttcgatgcgt acgcgcggtt tacgacgtac atacagcgca ccgatacctt tcgggccata
    30781 gattttgtga ccggagaaag acatcaggtc aactttcaac tggctcaggt cgataggcag
    30841 tttgcccacg ctctgggttg catcaacgtg atagataatg ccacgagcac ggcacatttc
    30901 gccgatagcc gcgatatcct gcaccacgcc gatttcgtta tttacgtgca tgatggaaac
    30961 aaggatggtg tcgtcacgca tcgctgcttc aagttctttc aggtcgataa tgccgttacg
    31021 ctgcggtgcc aggtaggtga cttcaaaacc ttcgcgctcc agctgacggc aggtatccag
    31081 taccgctttg tgttcggttt tgctggtgat gatgtgcttg ccttttttct gataaaagtt
    31141 ggctgcacct ttgatcgcca ggttgtcaga ttcggttgca ccagaggtaa agacgatttc
    31201 acgcggatca gcgccgacca gatcggcaat ctgattacgg gcgatatcta ccgcttcttc
    31261 agcctgccag ccgaaacggt gagaacgaga ggccgggtta ccaaaggttc cgtccatcgt
    31321 cataaactgc atcattttct cggcaacacg cgggtccacc ggcgtggttg cggagtagtc
    31381 gagataaatc ggtaatttca ttgctctata aactccgtac atcactcaat gcaaggaacc
    31441 aggctaccgg ctggatgtac gaccgtgttt acggagcgcg atacgccccg gcctgattct
    31501 aatatcttgt tatctttaac ctaactgttt cgggtttcag caagttgaca aacttacgaa
    31561 tctccgggag cttacataag taagtgaccg gagtgagtat gtgcagtcaa cgcagatgca
    31621 acacgaaaca ggacggttaa attaagcgcg taacttaacg tcgatcgcgt cttgtatgcg
    31681 ggtgcgtggc gcgtcgtgag tatgctgacg accagacaca tccagcactt cctggttatt
    31741 aaccagttcg cctaaagtaa tgttgttgag aaaaccggtg agacggtcgc tcaaatcacg
    31801 ccacagcgcg tgggtcaggc atttatcgcc gccctggcag ccgcctttac cctgacaacg
    31861 ggtggcatct acagattcgt caacggcgct aatcacttcg ccaacggcga tgctgctggc
    31921 atctttgcct aacagataac caccgcctgg tccacgtacg ctggaaacca gaccattttt
    31981 acgcagacgg gaaaacagtt gttccagata agaaagggaa attccctgac gttcggaaat
    32041 atcagccaac ggtaccgggc ccgcttcaga gttgagcgca acgtcaagca ttgcggtcac
    32101 ggcatagcgc cctttagatg tcagtctcat gtcttacttc acctcaaact cgcccctgcc
    32161 cggggttttt tattgtaaaa gtgggggtat tgcatagcag ggtcaagtct gacattcccg
    32221 agtaaattgg tcaactattt acttgactga tttagtcggg tatttaacct tcagtgccat
    32281 ttttttatcg tggcgtttgt acctgttgtc cttccagacc atgggcgggt tgagggcaat
    32341 tattcgcatt agcgctggcc tcttcccctc accctaaccc tctccccgaa ggggcgaggg
    32401 gaccgtccgt gcatttatct ggcttagccc tcaggcctta ttacctttat tctgctgctc
    32461 aatagaagcc agaatcccgc gcaggatatt caattcctgg ctttccgggc gcgcacgggt
    32521 aaacagacgg cgcaatttat tcatcacctg ccccggatgg ttttcacgga taaaaccggt
    32581 tgccagcagc gtttgttcca gatgaccata aaaacgctcc agatcatcga ccagcggata
    32641 cggcgtctct tcatgctcga cctgctcgcc gttttcctga gttgccagcc aggccatgcg
    32701 cacttcatag gcaataacct gagccgccat cgccaggttc agcgagctgt attccgggtt
    32761 agccgcaatc gcgacatgat aatggcattt ctgcaactct tcattggtca agccgacgcg
    32821 ctcgcgacca aacaccagcg ccaccggggt atttgccgct tcagcgacgc ttttcaggcc
    32881 gcattcgcgc gggtcgagca tcggccatgg cagcgtgcgg gagcgtgcgc tggtgcccac
    32941 caccaggcta caaccggcta acgcttcgtc gagcgtatcg acgatgtgag cattaccaat
    33001 cacatcgctg gcccctgctg ccagggcaat cgcctgggag tcgggtttca ccagtggatt
    33061 aaccagccac agattggtta atcccattgt tttcatggca cgggcaacag aacccatatt
    33121 gccggtgtgt gacgtctcca ccagcacaat tcgaatattt tgcagcattg tctttcttca
    33181 tctaaagatt attcacgcat cttatcataa aacgaagaca gatgccgatc tcgctgctat
    33241 actctgcgcc gttttcccgt tctttaacat ccagtgagag agaccgatgc atccgatgct
    33301 gaacatcgcc gtgcgcgcag cgcgcaaggc gggtaattta attgccaaaa actatgaaac
    33361 cccggacgct gtagaagcga gccagaaagg cagtaacgat ttcgtgacca acgtagataa
    33421 agctgccgaa gcggtgatta tcgacacgat tcgtaaatct tacccacagc acaccatcat
    33481 caccgaagaa agcggtgaac ttgaaggtac tgatcaggat gttcaatggg ttatcgatcc
    33541 actggatggt actaccaact ttatcaaacg tctgccgcac ttcgcggtat ctatcgccgt
    33601 tcgtatcaaa ggccgcaccg aagttgctgt ggtatacgat cctatgcgta acgaactgtt
    33661 caccgccact cgcggtcagg gcgcacagct gaacggctac cgtctgcgcg gtagcaccgc
    33721 tcgcgatctc gacggtacca ttctggcgac cggcttcccg ttcaaagcaa aacagtacgc
    33781 cactacctac atcaacatcg tcggcaaact gttcaacgaa tgtgcagact tccgtcgtac
    33841 cggttctgcg gcgctggatc tggcttacgt cgctgcgggt cgtgttgacg gtttctttga
    33901 aatcggtctg cgtccgtggg atttcgcggc aggcgagctg ctggttcgtg aagcgggcgg
    33961 catcgtcagc gacttcaccg gtggtcataa ctatatgctg accggtaaca tcgttgctgg
    34021 taacccacgt gttgtgaaag ccatgctggc gaacatgcgt gacgagttaa gcgacgctct
    34081 gaagcgttaa tgactcaggc gggtgatgtc actcacccgc cctcgccttt caggcgctat
    34141 tctgaaatac ttcctcaccg ctttactttc tttcgtcact ctcccaccat ctttccccga
    34201 ttaatggatg ataaagaagt cgcaaggaaa ggttatggca ctgccagtga acaaacgcgt
    34261 tcccaaaatt ctgtttattc tctttgttgt tgccttctgc gtttatttag tgccgcgcgt
    34321 tgccatcaac ttcttctatt atcctgacga taaaatttac ggtcccgacc cctggtcggc
    34381 ggaatccgtc gaatttacgg ctaaggacgg tactcgtctg caaggctggt ttattccttc
    34441 ttcgacgggc cctgctgaca acgccatcgc aaccatcatt catgctcacg gcaatgccgg
    34501 aaatatgtcc gcccactggc cgctggtcag ttggttaccc gagcgtaatt tcaacgtttt
    34561 tatgtttgat tatcgcgggt ttggtaaatc aaaaggcacg ccgtcccagg ccggattgct
    34621 ggacgatacg caaagcgcca tcaatgtggt gcgccatcgc agtgatgtaa acccacaacg
    34681 gctggtgctg ttcgggcaga gtattggcgg ggcgaatatt cttgctgtta ttggtcaggg
    34741 tgatcgtgaa ggcatacgtg cggtgatcct cgactccaca tttgcctctt atgcaaccat
    34801 cgccaaccag atgatccccg gcagtggcta cttacttgat gagagttaca gcggcgaaaa
    34861 ttatatcgcc agcgtcagcc cgatcccgct tttactcatt cacggtaaag ctgatcacgt
    34921 tatcccatgg cagcacagcg aaaagttgta tagcctggca aaagagccaa aacggttgat
    34981 cctaatcccg gatggcgaac acattgatgc tttttccgat cgccacggcg atgtttatcg
    35041 cgaacagatg gtggacttta tccttagcgc gttgaatccg cagaactaac ccatgatcgc
    35101 tagcacgata atcattcaca aaaccacctt aagacatgct aatccactgg tcagaacagt
    35161 ttaagatgag aaaaattctg tgacgcttgc caacatttct gatgattagc attcccttcg
    35221 ccatttccct gagcaaactt tagctattct tatcaattat gcttatggga gatctacaga
    35281 tgatgcctac gcttgctcca ccatctgtcc tttcggctcc ccagcgccgc tgccagatct
    35341 tgctgacgct ctttcagccg gggttgaccg ccaccatggc aaccttcagc gagcttaatg
    35401 gtgtggatga tgatattgcc agtcttgata tcagcgaaac aggacgggag atcctgcgct
    35461 atcatcaact cacactgacg actggttatg acggtagcta ccgggttgaa ggtacagtgc
    35521 ttaaccaacg tttgtgttta tttcactggc tacgacgtgg tttccgtctg tgtccgtcat
    35581 ttattaccag ccaattcacc cccgccctga agagtgaact gaagcggcgc ggaattgcgc
    35641 gtaactttta cgacgatacc aatctacaag cgttagtgaa tctctgctcc cgacggctgc
    35701 aaaaacgctt tgaatcgcgc gatattcatt tcctgtgtct gtatctgcaa tattgtttgc
    35761 tgcagcacca cgctggaatt acgcctcagt ttaatccgct ccaacgtcgc tgggcggaat
    35821 cctgccttga atttcaggta gcgcaggaaa ttggacgcca ctggcagcgt cgggcgctcc
    35881 agcctgtacc gcctgatgag ccactgttta tggcactact tttttccatg ttgcgggttc
    35941 ccgatccact gcgggatgcg catcagcgag acagacaatt gcgtcagtct atcaaacgtc
    36001 tggtaaacca ttttcgtgag ctgggaaatg ttcgttttta tgatgaacag gggttatgtg
    36061 atcagcttta tacccacctc gcccaggcgt taaatcgcag tttgtttgcc atcggtattg
    36121 ataataccct gccggaagag ttcgccagac tgtacccacg cctggtgcgc accacccgcg
    36181 cggcgctggc cggatttgaa agtgaatacg gcgtccatct ttctgatgag gaaagtggtc
    36241 tggtcgcggt gattttcggt gcctggctaa tgcaggaaaa cgatctgcat gaaaaacaga
    36301 ttattctact gaccgggaat gatagcgagc gagaagcgca gattgagcag cagttacgcg
    36361 aactaacgtt actgccgctc aacattaagc atatgtcggt aaaggcattt ttgcagacag
    36421 gcgctccgcg tggcgcggca ctgattattg cgccttatac catgccatta ccgctctttt
    36481 caccaccgct gatctatacg gacctgacgc tgacaacaca tcaacaggag cagatccgca
    36541 aaatgctcga atcagcatga gggaacaact ttcgggcgca aaaacattgc cggcagcgcc
    36601 accagcgcca ttacccagaa cacgccgtgg cccagatatt gatacaggaa accggcgaaa
    36661 acggtcatga tagcgatact gccgcccatc gcgacggcag agtaaaccgc ctgtaaacgg
    36721 atgacttcgc taccctggcg agcagcaata taacgcatgg cggccaggtg gcagaccgtg
    36781 aaggtgccgc aatgcagaat ttgcaccact atcaaccacg gcaacgccgt agttgctccc
    36841 ataatgcccc agcgcactac gccgcaaatc gccgagatca acagcatatc gcgtgcacta
    36901 caacggcgga aaagtttatt actcagcgca aagataatga cttccgccac cacgcccagc
    36961 gaccacaaat accccaccgc cgaggccgag tagccagctg cctgccagta aatggcgcta
    37021 aaaccgtaat aggccgcatg tgccccctgc aataaacaaa cgcaggccag aaagcgccag
    37081 ttctggcgaa ccagcgccaa ccacgcagac caaccggtgc tctcctgctg gcgacttgcc
    37141 ccttgtggct gaatcgtcgg acggatgaga aagccgagca gcatggatgc cacgcccaac
    37201 gtcaacagcg cgaggatcac ccgataatca aacatagtga ccagtttgcc cgtcagcgcc
    37261 gagccaatga caaacgccac cgagccccac agtcgcactt tgccgtaatc aagcgggaac
    37321 tgcttttgcc acgtattcgc cagtgcatcg gtcaacggta ccagtggtga gaaaaagagg
    37381 ttaaagccaa tcatcaccag catcagccac gctacgtgcg cccccgccca gaaggcgaca
    37441 gcaaagagaa gtgtcagcag tgccagcacg cgcaaggcgg aaatcaggcg ggaaggatcg
    37501 ctgacgcggg gcgcgatgag caaactcccg aggaaacggg caaccagacc tgcccccaat
    37561 aacaggccga tggtttctgg cgttaaacca atccctttaa gccagacgct ccagaaaggt
    37621 agaaaaatgc cgtaactaaa aaagtatgtg aaatagccga gcgccaacca gcgcgtggat
    37681 tgcaaaacca tgagtccctc ccgtcaggag gcgttagtct ggggttaatc gaatgttggt
    37741 gcaagttgaa aaataagaat attcgagcct gcttcccgat gtctgtaacg ccagttcaca
    37801 agcactaaaa aactgagatc cgcgacgcat gttgcaaaaa taatgcgtaa ttatgccgtt
    37861 acgcttgcca aacgttcctg cacaatggcg atgaaatcgc gtaatgccgg cttcatctcc
    37921 cctttcttcc acgccatcag taaagcaatg gaaggcacat ttccggcaat gggacgaaaa
    37981 acaacctgtc cggtattaaa attattcata taaccgggta tcaaagtgac gcccagcccc
    38041 atgcccacca gattcatggt caccagaata ttcgttgcca cctggacgat atttggctgg
    38101 ctgttttctt gcgcaaacca cgctttaacg atcggcgcaa gcgaaccgga atacaccgga
    38161 tcggtactga cgaaattcac gccatccagt tgcgcggcgg tgatctcttt ttcatgtgct
    38221 aaagggtgat caaccggtaa cacaaccact aatggttcgt caaaaagctc cagataatca
    38281 atctccgggc tataaacagg atggcgcatt aagccgacgt cgagttcacc acggcgaatt
    38341 ttttcctcct gttgcgtggt gattaaactc accagctcaa tcaaggtgtc tggctgtctg
    38401 agacgaaaca tcggtaatac ttttggcagt aaattcactt ccgccgatgg cacaaagcca
    38461 atggttaatt gtctgtcttc ctgaacaatt ttccgcgccc gtaatttggc attttccgct
    38521 tgttcgagga ttgccagcgc gtcctggaga aaacattctc ccgccgccgt caacgcgact
    38581 ttgcgcttat ccctcaccag taacggaaca ccgacacagt tttcaagatc gcggatctgg
    38641 ctgcttaacg aaggctgtga ggtatgcagt ttttccgccg cacgggtaaa gttcagtgcc
    38701 tgcgccactg cgacgaaata gcgtaaatgc cgtagttcca tcaccttccc cttgttatcg
    38761 aaaaaacgtc tcagctggta gaaattaact atttcacata ttagcaacca accagcaaca
    38821 tccttatggc acaaaaatag aaggtcaata catcttatct ttcaggatta aaaaatatga
    38881 ccacaccctc agatttgaac atttaccaac tgattgatac ccaaaatggt cgggtcactc
    38941 cgcgtattta taccgaccca gatatttacc aactggagct tgaacgtatt ttcggccgtt
    39001 gctggttatt tctcgcccac gaaagccaga tcccaaaacc tggtgatttc tttaacacct
    39061 acatggggga agatgcggtt gtcgtagtgc gtcagaaaga cggcagcatt aaggcgtttc
    39121 tcaaccaatg ccgccaccgg gccatgcgtg tgagttatgc agattgcggc aacactcgcg
    39181 cctttacctg tccgtatcac ggctggtctt atggcattaa cggcgagttg atcgatgtac
    39241 cgctggaacc tcgcgcctac ccacaagggt tgtgtaaatc ccactgggga ctaaacgaag
    39301 ttccttgtgt ggagagttat aaagggctga tttttggcaa ctgggatacc agcgcaccgg
    39361 gcctgcgtga ttacctgggt gacattgcct ggtatctgga tggcatgctg gatcgtcgcg
    39421 aaggcggcac cgaaattgtc ggcggcgtac aaaagtgggt gatcaactgt aactggaaat
    39481 tcccggcaga gcagttcgcc agtgaccagt atcatgctct gttcagccat gcttctgccg
    39541 ttcaggtatt aggggcgaaa gatgatggca gcgataagcg cctcggtgat ggacaaaccg
    39601 cccgcccggt gtgggaaacc gccaaagatg cgctgcaatt tggtcaggac ggtcacggta
    39661 gcggtttctt ctttactgaa aaaccggatg ctaatgtctg ggtcgatggc gcagtttcaa
    39721 gctattaccg cgaaacctat gccgaagcag aacaacgttt aggtgaagtt cgcgccctgc
    39781 gcctggcggg tcataacaat atattcccca cgctttcatg gctaaacggc actgccacac
    39841 tccgcgtctg gcatccgcgc ggccctgatc aagttgaagt gtgggcgttc tgtattactg
    39901 acaaagccgc ctccgatgaa gttaaagccg cttttgaaaa cagcgccact cgtgcttttg
    39961 gtcctgctgg ttttctcgag caggatgact cggagaactg gtgtgaaatc cagaaattgc
    40021 ttaaaggcca ccgcgcccgc aacagcaaac tgtgtctgga aatggggctt ggtcaggaaa
    40081 agcgccgcga cgacggcatt cctggcatta ctaactatat cttttcagaa actgccgctc
    40141 gtggaatgta ccaacgctgg gctgatcttc tgagtagcga aagctggcag gaagtgctcg
    40201 ataaaaccgc cgcttaccag caggaggtga tgaaatgagt gcgcaagttt cactagagtt
    40261 acatcaccgc attagccagt ttctctttca cgaagccagc ttactggacg actggaaatt
    40321 tcgtgactgg ctggcgcagc tcgacgaaga gattcgttac accatgcgca ccacagttaa
    40381 cgcgcaaaca cgcgaccgcc gcaaaggcgt ccagccaccg acaacctgga tttttaatga
    40441 caccaaagac cagctggagc ggcgaatcgc ccgtctggaa acgggcatgg cctgggcaga
    40501 agagccgccg tcacgcaccc gtcacttaat cagcaactgc cagataagcg aaaccgacat
    40561 cccaaacgta tttgctgtgc gggtaaatta tctgctttat cgggcacaaa aagagcgcga
    40621 tgaaacattc tatgttggaa cgcgtttcga caaagttcgc cgtctggaag atgacaactg
    40681 gcgcttgctg gaacgggata tcgtcctgga tcaagcggta atcacttccc ataacctgag
    40741 tgtactgttc tgatgaatcg aatttatgcg tgtcccgttg cggatgtgcc ggagggtgag
    40801 gctctccgga tcgatacctc gcccgtcatc gccctgttca acgttggcgg cgagttttat
    40861 gccattaacg atcgttgcag ccatggtaat gcgtcaatgt cagaagggta tctggaagat
    40921 gacgccacgg tggagtgccc gctacacgcc gccagttttt gcctgaaaac ggggaaagcg
    40981 ttatgcctgc ccgccaccga tccgctcacc acttatccag tacacgttga aggtggtgac
    41041 attttcatcg acttaccgga ggcgcagcca tgagcgatct gcataacgag tccattttta
    41101 ttaccggcgg cggatcggga ttagggctgg cgctggtcga gcgatttatc gaagaaggcg
    41161 cgcaggttgc cacgctggaa ctgtcggcgg caaaagtcgc cagtctgcgt cagcgatttg
    41221 gcgaacatat tctggcggtg gaaggtaacg tgacctgtta tgccgattat caacgcgcgg
    41281 tcgatcagat cctgactcgt tccggcaagc tggattgttt tatcggcaat gcaggcatct
    41341 gggatcacaa tgcctcactg gttaatactc ccgcagagac gctcgaaacc ggcttccacg
    41401 agctgtttaa cgtcaatgtt ctcggttacc tgctgggcgc aaaagcctgc gctccggcgt
    41461 taatcgccag tgaaggcagc atgattttca cactgtcaaa tgccgcctgg tatcctggcg
    41521 gcggtggccc gctgtacacc gccagtaaac atgccgcaac cggacttatt cgccaactgg
    41581 cttatgaact ggcaccgaaa gtgcgggtga atggcgtcgg cccgtgtggt atggccagcg
    41641 acctgcgcgg cccacaggcg ctcgggcaaa gtgaaacctc gataatgcag tctctgacgc
    41701 cggagaaaat tgccgccatt ttaccgctgc aatttttccc gcaaccggcg gattttacgg
    41761 ggccgtatgt gatgttggca tcgcggcgca ataatcgcgc attaagcggt gtgatgatca
    41821 acgctgatgc gggtttagcg attcgcggca ttcgccacgt agcggctggg ctggatcttt
    41881 aaggaagcac gatgaaagaa aaaaccatca ttattgtcgg tggcgggcaa gcggcggcaa
    41941 tggctgcggc ctcgctacgc cagcaagggt tcaccggtga gctgcatctg ttttccgatg
    42001 agcgacatct tccttatgaa cgacctccac tctcgaaatc catgttgctg gaagattccc
    42061 cgcagttaca gcaggtgtta cccgctaact ggtggcagga aaacaatgtt catctgcatt
    42121 ccggtgtaac catcaaaacg ctgggtcgcg acacacgaga gttagtgtta accaacggcg
    42181 aaagctggca ctgggatcag ctttttatag caaccggcgc ggcagctcga ccgctgccgt
    42241 tgcttgatgc actgggagaa cgctgcttta ccctacgcca tgccggtgat gccgccagac
    42301 tgcgagaagt tctgcagccc gaacggtcag tcgtgattgt cggtgccgga actattggtc
    42361 tggaactggc tgccagcgcc acgcagcgca gatgtaaggt gacagtgatt gaactggcgg
    42421 caaccgtcat gggccgtaat gcaccaccgc ccgtgcaacg ctatctttta cagcgccacc
    42481 agcaggctgg tgtgcgcatt ctgctcaata atgccattga acatgtggtc gatggtgaaa
    42541 aagtagaact gacgctgcaa agtggggaaa cgcttcaggc tgatgtggtg atttacggta
    42601 ttggtatcag cgccaacgag caactggctc gcgaggccaa ccttgatact gccaatggca
    42661 ttgtcattga tgaggcttgc cgcacctgcg atcccgcgat ctttgccggt ggcgatgtgg
    42721 caatcactcg tcttgataat ggtgcactac accgctgcga aagctgggaa aacgccaata
    42781 accaggcgca aattgccgct gccgcaatgt tagggctacc gctaccgcta ctgccgccgc
    42841 cgtggttctg gagcgatcag tacagtgata acttacagtt tattggcgat atgcgtggcg
    42901 atgactggct ttgtcgtggc aacccggaaa ctcagaaggc gatttggttt aatctgcaaa
    42961 acggcgtgct tatcggtgcg gtcacgctga atcaggggcg tgagattcgc ccaattcgca
    43021 aatggatcca gagcggcaaa acgtttgatg cgaaactgct gatagatgag aacatcgcgc
    43081 ttaaatcact gtaaccagga taattagcga atatctcaat gcctggggcg tggcgaggtg
    43141 caagagtgtg tattacgttt aaatcacatt atcttgcaaa gggattggtt atgaacacac
    43201 tacgttattt tgattttgga gctgcccgcc ccgttttgtt attaattgcc cgtatcgccg
    43261 tggtcttaat tttcattatt tttggttttc ccaaaatgat gggctttgac ggtacggtcc
    43321 aatatatggc ctcgttgggc gcgccaatgc cgatgctggc agcgattatt gcggtagtta
    43381 tggaagtgcc cgccgcgata ttaatcgtgc ttggcttttt cacccgtccg ctggcggtgc
    43441 tgtttatttt ctacacgctg ggtacggcgg tgattggtca ccattactgg gatatgaccg
    43501 gcgatgcggt tgggccaaat atgattaatt tctggaagaa tgtcagtatc gctggcgcat
    43561 tcctgctgct ggcaattacc gggccggggg caatttctct cgatcggcgt taggaaagat
    43621 gccggatgcg gcgtgaacgc cttatccggc atttaataaa ttacagccac tctacccgca
    43681 acgacatctc tgaagttgtt gattcccctg gtgctagcgc aatgagatca cccccttccg
    43741 ggcgatgatg atcatccggc gcatggctca tcggttccag acagaagaaa tcaaacgcat
    43801 atcctttatc aaacgcaggg tcggaaacaa agatgaaata acacggtgca ggtggcgtcg
    43861 tttccatgat gatggcataa ccttcctgcg gctgctcgat gcgggcctga ccattccatc
    43921 cggcaaaacc attgttcacc cactggcgcg gcaacggcgc gggctggtta aaatccagtt
    43981 cctgcggtag ctgctcgcaa aactcacccg ccagccactg ctcccgctcc agccagtaac
    44041 cgctcgcctg tgcctgaatc cgcgtttgcg gcgacaacgg aaaataagga tgccagccgg
    44101 taccaaatgg cagcgtctct gccccttgat tggtgacaga gagcgtcacc gtcagcgtat
    44161 ccgccgttaa atgaaacgcc tgacttaccc gatagtgata gacaccgctg cgatgttcat
    44221 acaccagaca caaactatca tcgctgtgcg agacacattg ccactcgccc agccagccat
    44281 cgccgtgtag atagtgcgca tcccactcaa cattcggttg cagttgatac tcacgcccct
    44341 gccagacaaa acgattgccg ctcacccggt ttgcaaatgg caccagcgga aaacatgagg
    44401 catcggttgc cacaccgctt tttttaccag gacgtaagag cggcgtcgta tcgcgccaga
    44461 agccttcgat tacgccgccc tggtcagaaa cgtccagctt tagcgacccg tgggataagg
    44521 tatagatggt catccgctaa ctccttaatc cgggaagtta atcacaactt tcccgcattt
    44581 gccgctcgcc atcagcgcat aggcatcacc tgcctgttcc agcgagaagc gatgggtaat
    44641 ggcgttacgc ggccacagtt tccagtccgt cagatcatgg gcgcattttt ccatatggaa
    44701 cagactggtc acccaggagc cgataatccg ccgttgatgg tgcatcagat cggcgctgac
    44761 ctcgaactcc acttttccgg tttctccgat gtaaaccacc cgtccccagt cagcggtgga
    44821 tcgcagtgcc agcaagcgac ctgcggcatt accagaacaa tcgagcgcaa catccgcgcc
    44881 accgtgggtg agttcggcga taatctgcgg cagaccttcg gtggttgcta aatagccgtg
    44941 atccatcacc cctaactgtt ttgccatcgc cagacgttcc ggcagcatat caacgccgat
    45001 gatccgtttt gcaccgcgac ctttcgccag catcatcgcc atcatgccga ctggccccag
    45061 accgaccacc agcacgttat cactgccgga aacttcgccg cgcaaaattc cttcatacgc
    45121 tgtaccaacg ccgcaactga taaacgcacc atcttcgtag ctcagcgcat ccggcaggag
    45181 gatcagatct ttttcttccg ccagcagata ttcggcatga ccgccgtcac gctgccagcc
    45241 gtaagccgct tttcctttgc cagtacaaga gataggaaag ccgcggcggc agttcgggca
    45301 aaaaccacag ccagaaatgt gatacaccag cacgcggtcg ccctctttaa aatggcggca
    45361 gccttgcccc atcgccacaa tctgcccgca cggttcatga ccgttgataa atccctggta
    45421 taacggttta tcgggtgccg ccgccgtggc acggtgttga tgatagatat agtggacatc
    45481 gcttccgcaa atcccggagg atttcatttt gatcagtacc tggttaatcc ccggcgtcgg
    45541 caccgcaact tcccgcagat cgacggttga atttcctggt aaataagctg ccagcatcgt
    45601 tttcataaat ccctcattaa caatacgatt aattttcatc cctgccgcac ccgcgccagg
    45661 ggcgttaagg tttagcgttt cgctttactg cttcgctggg tcagcaagat attcgccagc
    45721 accgccacca cgatgatgac gccgcgtacc acctgctgga aaaaggagtt aataccgagc
    45781 agcaccagac cgttaccgat tagcgtaatc accagcacac caagcaatgt accgaacagg
    45841 gaaccgcgac cgccggaaag tgccgtaccg ccgaccacga ccgcggcgat gacgtcaaac
    45901 tccagaccgt ttgcggcacc tgcgttaccg gaaccgaggc gtgccgccag caaaatgccg
    45961 gtcaccgccg ctaataatcc cgaaagggta aagataagaa tgcgcactcg acgaacgtta
    46021 ataccgcaca actgcgccgc cgtggcatta ccgccaacgg caaaaaccga gcgcccgaag
    46081 gcggtttttc ggctaatgaa cacaaacagc gcaaacaaca ccatcatgat cagcgcggat
    46141 actggcacac cgagaaattg tccgcccagc cagtccagca cctcgttttc gtcaatcggc
    46201 actggcagtg cgttggtcat aaacagcccc attccgcgca gggcgctcca cagccccagt
    46261 gtggcaacga aacttggtac gttaaacacg ccgcgcagca ccccggcgag cgttcccatc
    46321 agcgcgccta acagcaacac cagcagacac gccaccgcca gcggaacttc aaattgcagc
    46381 aaaaatgcca ggcacaccga gacaaaagcc accatcggcc caacgctgac atcaatttca
    46441 ccggagataa taatcagcgt catcgcccag gcggcaatcc caatggtggc ggcatcgcgc
    46501 agcacgttca tctggttatt caatgagata aagcctggcg cgttcaggga gaagaccaga
    46561 taaagaatgg caatcaccac cagcaaaccg atctcattaa tatggcgact gacaaattgt
    46621 ttgagcgaga cgctcttgcc ctgcggcaat ggtaatgacg aagcagacat cttcaggttc
    46681 ctcggtaaaa ttcatcagtg cacagacaga atggcggaca tcagctcatc cacattgacc
    46741 ggagcgtgaa actcctgcga gaacgtgccg tgctgtagta acaggatgcg gtcacagacc
    46801 agcggcaact cctccacttc actggagata aacaccacgc tttttccttc ggcagccagc
    46861 tcacggacaa tacggtaaat ctgctgtttg gcttcgatat cgacgccgcg cgttggctcg
    46921 tcgagcaaca aaatctggct ggcggcatag acccaacgac cgatcaccac tttttgctga
    46981 ttgccgccag aaagcgtgcc gatgggcgtt tcgctactgg cggccttgac cgtcatccgc
    47041 tgcatcacct cttcggtcag gcggcggatg gtggaccatt gcagtacgcc gttggcgctg
    47101 attttttgcc gattggtcag cactgtattt tcgtcaacgc ccaaccaggg aatgatcccc
    47161 gcttctttgc ggttttctgg cgtatagcca atgccgcgtt tcagcatgtc gccgtaatcg
    47221 gggcgcgtga ttttctcgcc gttgataaca atttcgccct gttcatactc ctccagccca
    47281 acaatcgcct tcagcaattc actgcgccct gcccccagca ggccagcaat gccaagcact
    47341 tcgccgcgac gtaacgtaaa gctgatatcc tccagcttgg gcttatggcg taatgcacgg
    47401 acttccagca cggcctgatc cacaatttcc tgaggggcaa ccggcgcaat atcaacgtga
    47461 tcacgcccta gcattagcga cacaatatga tgcgtggagg tgttttcgag catcacatcg
    47521 cccgccacct gaccatcgcg cataacggtg gcacaggagg caatgcggcg aatttcttcc
    47581 atccggtggc tgacataaat caccgccacg cccagtgccg acatcttttt caccgcgatg
    47641 atcaccagtt caacttccgc actcgcaagc gaactggtag gttcatcaag aatgaccacg
    47701 cgcggctcgc ccttcatcac ccgcgcaatt tccaccagtt gcttttgcgc cgggcttagc
    47761 gttgaaacaa gttgttcagg gctaacgtca acgcccagcg cctgtaagca acgttgggca
    47821 tcctgcgcca tttgcaggta atcaatcatg ccgttgcggc ggggccactg accgaggcag
    47881 aggttttccg ccaccgtcag tccctccacc agacttaact cctgataaac cgcacgaacc
    47941 cccagttcag cggcacggcg agtcaacgta gcttcgtcac cttccagtcg cgtctcgcca
    48001 atccagatat caccgctatc cgggcgttcg ctgccggtaa gcattcgaat gagagtcgat
    48061 ttgcccgcgc cgtttttacc taacagcgca cgaacttcgc ctttattgag cgtgaagtta
    48121 acgttatcca gcgcaacgac gccggggtaa cgcttatttc ctgccaccac ttttgctacc
    48181 gggactgcct ctgttgccgt gaacataggg acctctgcga atcagcgatt agggcagacc
    48241 atcaacgtgc gttgccagcc actgtttacc atcttccgtt ttggtataga gatcgattgg
    48301 cacctgaatc actttttcac cgtcggcttg tttattgata accttcaatg tttgcgcgaa
    48361 aacagcattg cccattttct taccggaaat atccactacc gctttcagca cctgattgtt
    48421 ttccagctcc tgagcaattt cggttgtcat atccgaaccg aaaacagcaa tttttccggc
    48481 ctgattttga ttacgtaccg cttttaccgc gccgagtgtc gcaccgcccg actcccccat
    48541 aatggcgttg agatccggcg tggagataat cagtttttca ccaacggaaa tcgctttatc
    48601 taaaacagtc ccttcctgat tagcgacaat ttgcgcgccg ggaacgcggg attttaatac
    48661 ttcttcaaat cctttacgtc gctgcacaca aacttcaaag gcttcgcaat tgatgacagc
    48721 aattttcggc tggtcaattt tattggcaat aaaataatcg gcggcagcgt tacccagttt
    48781 tttaccaaat tccagcggat cgccgaccag atacgccgag acatatttat cgcccccctt
    48841 ttgattaata caggtgttgt agcaaatcac cggaataccc gcttcactgg cgcgacgaac
    48901 ggtacggcta ctgccatttt cagacactgc cgataaaata atggcatcga cattacgcgc
    48961 cacgagggta tcaacaaagg tactttcttt cgaaatatca ccctgggcgt tagtttcaat
    49021 taactgtacc tgtactgaag aatcttttgc cgcatcctga acgccctgac gcactccagc
    49081 gtaatatccc tgggtatcaa ggtatattgc accaatggtc atttctttat ccgcagccct
    49141 ggcaaatagt gcgctaccta atagcgtagc cattaataat agattacggg ttgttctcat
    49201 ttttttaggc atagttcctc ctgtagggtt tttattaaca acggcttatt ctaattattt
    49261 tgtgatgagc ggcagcgcgt gccgctcaaa tattacagtt aatagggttt agtgaacgta
    49321 gttaaaaata aaaggcatca cggtggtgaa taatgccgct ttcggccagg ccggattaat
    49381 cgccgtcaat tcagcgcgag cagattcata ttgcgcaacc tccccgagtc ccgcggacgc
    49441 cagcatcgcc accatcaggc atttttcttt atgctgctgt tgtaaatcgc catacagcga
    49501 caacaggtca ggttgtgaaa cagcaaagaa atccgcctca atactggttt tcgccatctc
    49561 ttgcgcccac tgtttcattt cactaaacag ttgctgtgcg gtttgctgtt cacccagcag
    49621 tcgcagcgcc attccttgcc agaagagata atcaaccggc tgatcgttgt aataactgtg
    49681 gatgttaatg gtgcgatcgc cggtcgccgc cagacgtaaa caacgcatcg cttcagtttc
    49741 atcgccctgc gcgttggcgc atatcgcctg ccagaaccag atgtcgttat cagtttgccc
    49801 cggtaaacgg ccttcgctta aattctccgg ataatgcagc gcggcatgaa gcagttcgct
    49861 ggcctgctgc ggctctctgg catcaagatg ctgccaggcg cgtaataact ggttgaggat
    49921 aaactgactg gtgaccttcc cttccccgcc ttcccacggg tggaatttac gcgtggcgag
    49981 aatgtccgcc gctttgtctg cctgacccgt gagatgccac aaattgagca gttctgcggt
    50041 catgtcgtcg cgtttcagcg caatttcctg attattttcc agacgcgcca gtcgtttctc
    50101 cggtgtggct ccacttaact tatcaagcaa atcccgttcg aaaagcagac gtgcatcctg
    50161 cggcgcaagc tgataagcat tatcaagata acgcgcggcc agctcataat cgtgttgctt
    50221 attccacgca tggatcgcta acccgcgcca gccgtcggca aactccggcg acatctctac
    50281 gcaacgttgc cataaggcaa tggctttgtt gtagctacgt ttgttgtagt agaagcaggc
    50341 cagtaaatgg cgagcaaacc agcactcttc aatactctcc agcgccgcca cttcttccag
    50401 cgtattcggg aaacggacaa actgcgggaa gacatcaatg gcttttgcga ccagttcgcc
    50461 acgttcggct ttcggcagca agctggcttg caggtaaagc ggcagcgtgc gctggcagtc
    50521 cagtgcgttc agcatctctg ccgccagggt gggcattccc cagttaatca gttgcccggc
    50581 ggtcatcagg gcgttaacgt cgcgtccctg acacagaccg cgccactgcg cgagagctga
    50641 ttcgctacga ccatcgaacc agttcagcca ccacagagtg gcgttcagcg gataatcgcg
    50701 cagcagtttc tcgcgctgca cacgcgcgtt gtcctgacga ccacttaaca ccagcagcag
    50761 attatgcagg caaagcactt cctgattggt tgggcaggcg cgaagacttt gttggcaaaa
    50821 atccagacca gcgtcgaagt taccattacg cgccgccagt cgtgccaggc cataatagcc
    50881 accggctttg ctgttgccgc tccagaccgc acgccagaaa tcctcttcgg cttgttgata
    50941 ttgtccctga cgttcgtaag cactggcgcg aatcaaactc gcctgtccgc actgcggatt
    51001 tttgttcagc gcatgtgcgc gtttcagagc ctgagtggca tacgccaccg cttgcgggaa
    51061 atcggcgcgg ttatattcca gcatcgccag cgccaggtta cagcggtaat ccagcggatc
    51121 cagcgccacg ccgcgcaggt agtaatcgaa cggtgaacgg ctggcgtgat gatattgctc
    51181 cagatgctga ccgataaacc aggcttcatc tgtactggta atgtcttgtg ctgccagtgg
    51241 cgctttggcg acgtccggca acggcaactc ttgcggctga tgttcctgat aactcagtac
    51301 aatattgccg tcggcatcgg agagctcaat ggtcagcctt tcaggattga taccgtgcaa
    51361 cacgccctgg atggcggtcg caggcatcag tgccacggca tcatcaagta acgcgttgca
    51421 tttgccgatt tcgcggatcg ccaggcggta tccgttcaac ggagagatgg catacagccc
    51481 ccactcaatc ccccgcttac tacgctggag ttttatcacc gcatcgcggg aggcattttg
    51541 caccatgccc aaagaatgat aaggcaggaa atactgctca aaacgcttct cttcgtaagc
    51601 atcaagccag gtaaaatcgg gctggttatc ggcaaaaata ccggtcatca gttcgatata
    51661 cggaccgtta ttgtcggtca gactcttatc ccacgcctgg ccaaattcac tgtgtcccca
    51721 actccactgt tttttacctg gcgcaatatg gtggttggca acgtgcagca aaccgccatc
    51781 ttcatcgtga caccacgcgc cgacaaaatc gtactgcgat ttttcggcca tatatgaggt
    51841 tggaacaggc acatttttat agcgagaaat gtccactccg gcggagtagt ccactttgta
    51901 gtaagtgccg gtggcgatgg ggaaagcgga gacggcccgt ttgccgtgat caaacaccgc
    51961 agtcacatcc ggtgggaaaa cgctctgatg cccttccccc cctttcactg ccgggttggc
    52021 ccaccacaag aaatgacgcg gcgtggcgtt accgttatag acgcggctgg cgatttccag
    52081 cgccgcccgg tcagggcgca gggtgaaacc tgtcatcacc tgtaaaccat gcatcggctc
    52141 cgtttcgcct acccacaccg tctgtgcacc gtcttcatgg gcttcgaggg tgaaatcaac
    52201 gggcataaag gtggtcgggc gatgatgttg cggccagtta aactcaatcc caccggaaat
    52261 ccacggcccc agcagcccca ccagcgcagg tttaatgact tcattgtgat aaacaaaatc
    52321 gcgctgtttc actttatccc acgcgcgatg cacccgaccg cccagttccg gcaggatcat
    52381 cactttgatg tagtcgtttt ccagccacac cgcctgccag gatttcaggg ttttctgctc
    52441 gctcagcgta tcggtcacgc cgtagggata aaccgcgccg gacgatccct gataaacgcg
    52501 attttcgagg aacatgggat gtatatcctg cggcccggtt tcataggtcg ggatctcaac
    52561 gcgctcttgc cacactttta ctggagtcat ggtgccctca atattaacaa gacatactga
    52621 attaaaagat ttgtggcagt gtattgaaca atctggcaat gttttcgcgg aataatcacg
    52681 caattaacta aacaaggttt agtgaagatg agagcctgca ttaacaatca acagattcgc
    52741 caccataaca aatgcgtgat tctggaactg ctgtaccggc aaaagcgcgc caataaatca
    52801 acgctggccc ggctggcgca aatttcgatt ccggcagtca gtaatatttt gcaggaactg
    52861 gaaagcgaaa aacgggtggt gaatattgac gatgaaagcc agacgcgcgg gcatagtagc
    52921 ggtacatggc tgattgcgcc ggaaggtgac tggacgctgt gcctgaacgt gacgcccacc
    52981 agtattgagt gtcaggttgc taatgcttgt ttaagtccga aaggtgaatt tgagtattta
    53041 cagattgatg caccgacgcc gcaggcgctg ctgtccgaaa tcgaaaaatg ctggcatcgc
    53101 caccgtaaat tgtggccgga ccgcaccatc aatctggcgc tggcaatcca cggtcaggtt
    53161 gatcctgtga ccggcgtgtc gcaaaccatg ccgcaagcgc cgtggacaac gccggtggag
    53221 gtgaaatatc tgctggaaga gaagctcggc attcgggtga tggtcgataa tgactgcgtg
    53281 atgctggcgc tggcggagaa atggcaaaat aattcgcagg aacgggattt ctgcgtgatc
    53341 aacgttgatt acggcattgg ctcgtcgttc gtgattaacg agcaaattta tcgcggcagt
    53401 ttgtatggta gcggacagat tggtcacacc atcgttaatc cggatggcgt cgtctgcgac
    53461 tgtggacgtt atggctgcct ggaaaccgtc gcctcgttaa gcgcattaaa aaaacaggcg
    53521 cgggtatggc taaaatcaca accggttagt actcaacttg atcctgaaaa actgactaca
    53581 gcgcagttaa tcgctgcctg gcaaagtgga gaaccgtgga tcaccagctg ggttgaccgc
    53641 agtgccaatg ccattggttt gagtctgtat aacttcctca acatcctcaa tattaatcag
    53701 atttggttgt acggtcgcag ttgtgccttt ggtgagaact ggcttaatac tattattcgc
    53761 cagacaggat ttaacccgtt cgaccgcgac gaaggaccga gcgtgaaagc gacgcaaatt
    53821 ggcttcgggc aattaagccg cgcacaacag gtgctgggaa ttggctattt gtatgttgag
    53881 gcgcagttac ggcagatttg atggcgcaat aacgtagaaa ggcttcccga aggaagcctt
    53941 ttttcaacag attaaccgtt tcgcttatgc gtaaaccgga taacgtgcgc agatgtcgag
    54001 aactttacct ttgatgcgct cgataacagc ttcatcattg atgctgtcca gcacgtcaca
    54061 catccagcca gccagttctt tcgcttctgc ttctttgaag ccgcgacgcg taattgccgg
    54121 agtacccacg cgaataccgg aggtcacaaa cgggctcttc ggatcgttcg gtacgctgtt
    54181 tttgttgacg gtgatgttag cacggcccag agcggcgtct gcttctttac cggtcaggtt
    54241 tttatcaacc agatcaacca ggaacaggtg gttatcagtg ccgccggaaa ccactttgta
    54301 gccgcgctcg aggaacactt ctaccatcgc tttagcgttt ttcgcgacct gctgctggta
    54361 agttttgaac tcaggctcca tcgcttcttt cagagcaacc gctttaccgg cgattacgtg
    54421 catcaacgga ccgccctgac caccagggaa aacggcagag ttcagttttt tgtacagctc
    54481 ttcgctacca cctttcgcca ggatcaggcc gccgcgcgga cccgccaggg ttttgtgagt
    54541 ggtggtagta acaacgtgag catgaggaac cgggttcggg tagacgccag cagcaaccag
    54601 gcccgcaacg tgcgccatat caacgaacag gtaagcaccg atgctgtcag cgatttcacg
    54661 cattttcgcc cagtccacca cgccggaata tgcagagaaa ccaccgataa tcattttcgg
    54721 cttgtgttct ttggcttgtt tttccagatc ggcgtagtcg atatgaccgg tagcatcgat
    54781 accgtaagga acgatgttgt acagtttacc ggagaagtta accggagaac cgtgagtcag
    54841 gtgaccgcca tgcgccaggt tcatacccag aacggtatca cctggttcca gcagcgcggt
    54901 gtagaccgca aagttagcct gggagccgga gtgcggctgg acgttagcgt agtcagcgcc
    54961 gaacagttct ttcgcacgat cgatcgccag ttgttcaacg atatcaacat actcgcaacc
    55021 gccgtagtag cgtttgcccg gataaccttc agcatatttg ttggtcagct gagaaccctg
    55081 cgcctgcatt acgcgcgggc tggtgtagtt ttcggaggcg atcagttcga tgtgctcttc
    55141 ctgacgtact ttttcctgct ccatagcctg ccacagttcg gcatcataat cggcaatgtt
    55201 catttcacgc tttaacatcc gcatctcctg actcagctaa caataaaatt tttggccttt
    55261 ataggcggtc ctgttggaca acggcgaaca gtataaccga atcattgtgc gataacaggt
    55321 cttgacaaag gaatttacgc aaacgattac cttcaggcta cgcaaggctt tggagaataa
    55381 agagcttgca accggaaacg gatttctttt caggtttgtg atgcaaattt ttcacttcat
    55441 cacattcttt ctgaaaaaca ccaaagaacc atttacattg cagggctatt ttttataaga
    55501 tgcatttgag atacatcaat taagatgcaa aaaaaggaag accatatgct tgacgctcaa
    55561 accatcgcta cagtaaaagc caccatccct ttactggtgg aaacggggcc aaagttaacc
    55621 gcccatttct acgaccgtat gtttactcat aacccagaac tcaaagaaat ttttaacatg
    55681 agtaaccagc gtaatggcga tcaacgtgaa gccctgttta acgctattgc cgcctacgcc
    55741 agtaatattg aaaacctgcc tgcgctgctg ccagcggtag aaaaaatcgc gcagaagcac
    55801 accagcttcc agatcaaacc ggaacagtac aacatcgtcg gtgaacacct gttggcaacg
    55861 ctggacgaaa tgttcagccc ggggcaggaa gtgctggacg cgtggggtaa agcctatggt
    55921 gtactggcta atgtatttat caatcgcgag gcggaaatct ataacgaaaa cgccagcaaa
    55981 gccggtggtt gggaaggtac tcgcgatttc cgcattgtgg ctaaaacacc gcgcagcgcg
    56041 cttatcacca gcttcgaact ggagccggtc gacggtggcg cagtggcaga ataccgtccg
    56101 gggcaatatc tcggcgtctg gctgaagccg gaaggtttcc cgcatcagga aattcgtcag
    56161 tactctttga ctcgcaaacc ggatggcaaa ggctatcgta ttgcggtgaa acgcgaagag
    56221 ggtgggcagg tatccaactg gttgcacaat cacgccaatg ttggcgatgt cgtgaaactg
    56281 gtcgctccgg caggtgattt ctttatggct gtcgcagatg acacaccagt gacgttaatc
    56341 tctgccggtg ttggtcaaac gccaatgctg gcaatgctcg acacgctggc aaaagcaggc
    56401 cacacagcac aagtgaactg gttccatgcg gcagaaaatg gcgatgttca cgcctttgct
    56461 gatgaagtta aggaactggg gcagtcactg ccgcgcttta ccgcgcacac ctggtatcgt
    56521 cagccgagcg aagccgatcg cgctaaaggt cagtttgata gcgaaggtct gatggatttg
    56581 agcaaactgg aaggtgcgtt cagcgatccg acaatgcagt tctatctctg cggcccggtt
    56641 ggcttcatgc agtttaccgc gaaacagtta gtggatctgg gcgtgaagca ggaaaacatt
    56701 cattacgaat gctttggccc gcataaggtg ctgtaatttg atgttgccgg atggaaacat
    56761 ccggcaaccc ttgacgcggt ttaaattgcc gcgtcgtcct cttcaccggt acggatgcga
    56821 atgacccgtg ccacgtcgaa gacgaagatt ttaccgtcac cgattttgcc ggtttgcgcc
    56881 gtgcgaataa tggtatcaac acaggtatcg acaatgtcgt ccggtacgac aatctcaatt
    56941 ttcactttcg gcagaaaatc caccatatac tccgcgccgc ggtacagctc ggtatggcct
    57001 ttctggcggc caaaaccttt cacctcagtg accgtcatgc cggtaatgcc gacttcagcc
    57061 agtgcttcgc gaacatcgtc cagcttgaag ggtttaataa tcgcatcaat ctttttcatg
    57121 ctattccttg aaaaggtcgc ctgtcttttg atctgctaaa cgtaacacat aacgccaatt
    57181 cattccttga aatcgtttgc atccagctcg tgtcgggaaa gcagtttata aaattctgtc
    57241 cggttgcgcc ccgccattct cgccgcgtgt gtgacgttgc ctttggtgat ttgcagcagc
    57301 ttacgcaaat agttgagttc aaactgatta cgtgcctcaa caaaggttgg cagcgccgta
    57361 ttttcaccct ccagcgcctg ctctactaat gcatcgctaa taaccggaga tgaggtcagc
    57421 gccacgcact gttcaatcac gttgaccaac tggcgcacat tacccggcca gctagcggtc
    57481 atcaggcgtt tcatcgcatc agtggagaac gcgcggacaa acggtttatg tcgctctgcc
    57541 gcctggcgca acaggtgatt tgccagaagc ggaatgtctt ctgtgcgctc tgccagagcc
    57601 ggaattttca ggctgacaac gttgaggcgg taatagaggt cttcacggaa ttccccgcgc
    57661 gccatcgctt ttggcaggtc gcggtgagtg gcggaaataa tccgcacgtc gatatcaata
    57721 tcgcggttac tgcccagcgg gcgcacttta cgctcctgca acacgcgcag cagtttgacc
    57781 tgcaacggtg cgggcatatc gccaatctca tcgagaaata gcgtaccgcc ttccgccgcc
    57841 tggaataaac cttcgcgatt gctgacagcg ccagtaaacg cgccacgcgc atgaccaaac
    57901 agctccgact ccagcaattg ctcgggcaat gcgccacagt taatagcaat aaatggtttg
    57961 ctattgcgcg ggctggcgtt gtggatagcc tgggcgaaaa tctctttccc ggtgccgctc
    58021 tggccgttaa tcaaaacgct aacgtctgat tgcgccacca gccgcgcctg ttccagcaaa
    58081 cgcagcatca gcgggctgcg ggtgacaatc gcctcgcgcc agcgttcatc ggtggctggc
    58141 gcggattgtt ccagcgcatc gtcaattgcc tgatatagcg catctttatc gacaggcttg
    58201 gtgaggaaac tgaacacgcc ctgctgtgtt gcagcaacgg catcgggaat agaaccatgc
    58261 gcggtaagaa taattactgg cattcccggc tgcactttct gaatttcagc aaacagctgc
    58321 ataccgtcca tttcatccat ccgcaggtcg ctgatgacta aatctacttt ttcgcgattc
    58381 agtacccgta atccttcagc gccactttcc gccgtgacca cgctgtagcc ttcgctggtc
    58441 aggcgcaggc caagcagttt cagcaatccc ggatcgtcat cgaccaataa taaatgcgca
    58501 ggtttatggc tcatcaggag tgacctcatg ggtggatggc gcgggctttt caccctcgtg
    58561 cggcgtatcc ggcgagaaat ttccagccgg tttgcgggtc gagagctggc gttcaatatc
    58621 agtcaggttt tccagcttgc gggtggtgag ttccagttgc tgctgtagaa cgtgatgttg
    58681 ctggcgcaat gtatccagct cgctgtcgct ggcctgctgg agcttgccgt agcgttggcg
    58741 ctcttccgcc agttgcagtt gcagcgcctg accgtcgcgc cagagttgat acagagggcg
    58801 aacctgtgcc gggatctcgg tacttagcgc atcaatgcgc gcgaccagct ggcggcgctc
    58861 atacggcgta attttggcgt cggcgagcaa aatcccttgt ttaaaggtat tttgccagct
    58921 gccgtcgtca tattggcgag cttgctgacg tgactgcgca ggcattaaac gatcagcaca
    58981 atccatcgcc cgcagccagt aaagcggatt ggtttcggtt gatttacctt gcagcgccca
    59041 gatgtcgcta cattcagtag aaagatagtc agccagttga taaaccggaa ttttttcttc
    59101 tgctggcgta tcaatggctg gcttattgtg actctgcacg caacccagca atgccagaca
    59161 tggcagccct gccagccaca gccgtcgggg caataatcgt tgaaaaatgt gtcgcatatt
    59221 caccagactt aatgcctatc ccagtgggcg taattattgc aggcagtttg gacatggaca
    59281 gcgcggagaa accggagcgt acacgtcgta cgtgaggatt tcgagcactg cccggggccg
    59341 aactggcaaa taaaatagcc tgatgggata ggctgttagc attatttcgt gtttttcgac
    59401 gacggcaatt caatgcggaa acaaacgtct tgcccgctct cgtcgaccag atacagttcc
    59461 ccttgcatac ggcaaataca atccctggca atgcttaatc ccagaccgct gcccttcacc
    59521 gccccttttc gctggtggct tccttgaaaa aagggttcga agatcatggc gcgttcctct
    59581 tgcggaatgg gtgtgcctgt attgatgaca tcaatataaa cccgtgcacc atgtaagctg
    59641 ctgcgaaggc aaatgttacc ggattcagcc ccgtagtgca ccgcattgga gtaaagatta
    59701 tccagtacgc tcatcagcag cattggctcc gccaggcaag ctgttgcttt gagatcgacg
    59761 tcggtatgca tcattttagc ccgtgcgggc aggctatgag cagaaaccac tgtctccacc
    59821 agcggtgcta attcaacatt ctccagttcc accgcactgt ccgcctgttt acggttgtaa
    59881 tcaagcagtt gttcgatcag tttttgcaaa ttgcggctgc tgctatcaag aatgctcacc
    59941 acctcttttt gctctggcgt aagcggcccg acaacctggt cagccagtaa ttcagtgccc
    60001 tcacgcatac tcgccagtgg cgtttttaat tcatgagata aatgtcttaa aaattgatgg
    60061 cgttgggatt ccagccatga caggcgctca cttaaccaaa gaatacgttg cccaaccgag
    60121 cgtaactcgc tcggtccact gaacgaaacg ctattgccca gagaacgccc ttcccccaga
    60181 cggttgatca tgcgctcgat atttttcacc ggcccgataa tcatccgcgt gaaaagtagc
    60241 accatcacca gactcaccag aaatagcacc agcgattgcc aaccaaaata ttgcccacgt
    60301 tcggcgattt cacgctgaag ttgctgccca cgagagaaca ccactgtgcg cgtggcctgc
    60361 accatttcgg tattggcact ggcaaaggct tcaagacgcg cggcggcggc agcatcggga
    60421 ccactgttgt tacactgaag ttgggccaga ttgttcaaat cctgacgcaa tgcctggtaa
    60481 agtttatcgt ctggcagtac gcctgcgtgg gcatcgagca tttcgctgta acgcttgcgc
    60541 tggctttgat aaaccttcgc aagcgtaggg tcgtccagca cgcaatactg acggtagcta
    60601 cgctccatct ccagcgccgc gttggtcatc gcttcactac gccgggcatc aataagcgta
    60661 gtgcggttaa ccagcgccgc ctgatcgcta agcgcattca ggctttgcca ggcttgccat
    60721 gccagcacca acaggggtag tagaattagc aaaaatgcca gcattaccag ttgtcgtaat
    60781 gagcggggaa aaacgggcca gcgtttcaag gtgttactct cgtcagacgc gaatagcctg
    60841 atgctaaccg aggggaagtt cagatacaac aaagccggga attacccggc tttgttatgg
    60901 aataaggcgg tgcctaactc gacgtttcgc ccgatggttg atatagctac gctgatatca
    60961 gaagttggac ggcaggcacc ttgttgtgcg tcattcgaat tttatgtagc acatcccgaa
    61021 ggggctgaca taagtcggtg aatgagccac tgcttactat tatgcagtaa ctgtgccaat
    61081 aaagaaaata gtttggtaac gtactgatta tacgttgctt tgagggattt atggcttctt
    61141 cgctatttga atgtaaatta gccactgtgt cgctaaaaag agacaactta agataaactt
    61201 attagattac aataaaatca atcatttaag tgtcgccaga aagcgacacg gtaaaccacc
    61261 cattgtcgtg atttacagac acaaaaaagc tcccggagtt gggagcttat gatagtggtt
    61321 ggtgcttaat gccggatgcg gcgtaaatgc cgcatccggg ctgcaaaacc aatgaactga
    61381 tgacttaccc caactgctta cgcgcattgc ggaaaatgcg catccatggg ccatcctcgc
    61441 cccagttttc cggatgccag gagttgctga cagtacggaa aacacgttcc gggtgcggca
    61501 tcataatggt gactcgacca ctttcagtcg taaccgacgt aataccgttc ggagaaccgt
    61561 tcgggttagc cgggtaggtt tcagtgactt tgccgaagtt atcgacatag cgcagtgcca
    61621 ccagcccttt gctttccagt gccgccagat gcgccgcatc acgcacttcc acgcgccctt
    61681 caccgtgaga gacagcaatc ggcatttgcg agcccaccat cccctgcaac agcagcgacg
    61741 ggctttgggt cacttcaacc aggctgaaac gcgcttcaaa gcgatcggag gtattgcgca
    61801 caaaacgtgg ccacaactca ctacctggga tcagttcacg cagattagac atcatctggc
    61861 aaccgttaca tacccccagc gccagcgttt gcggacggtg gaagaaggtt gcaaactcat
    61921 cgcgtacacg gtcattgaac aggattgact tcgcccaacc ttcaccggca cccagcacat
    61981 caccgtagga gaaaccaccg cacgcgacca gggcgtggaa atcttccagg cccgtgcgtc
    62041 cagccagcag gtcactcata tgcacgtcga tagcatcaaa gcctgcacgg tggaaagctg
    62101 ccgccatttc aacatgcgag ttcacgccct gctcacgcag tacggcaact ttcgggcgcg
    62161 cgccagtggc aataaacggt gctgccacat cttcgttgat atcgaacgac agttttacat
    62221 tcaggcccgg atcggcgtcg tttgatttcg cctggtgctc ctggtcagca cactccgggt
    62281 tgtcacgcag gcgctgcatc tgccaggtag tttctgccca ccagacacgc aacgtagtgc
    62341 ggctttcgct gaatacagtc tgcccgttgg cggtaatcac aaaacggtca ccggaaaccg
    62401 cctgcccaac ataatggacg caatcagcaa gcccatgctg tgccagtacg gactcgaccg
    62461 cttcacggtc agcggcacga acctgaatca ccgcacccag ttcttcgtta aacaacgccg
    62521 ccaggcgatc gtcacccaga gagccgatat ccgcatcaat gccacaatga ccagcaaagg
    62581 ccatttccgc cagtgtgacc aacagaccgc catcagagcg gtcgtgatac gccagcagct
    62641 tacgctgtgc aaccagcgcc tgaatcgcgt catagaagcc tttcagttgc gcgacatcgc
    62701 gtacatctgc cggtttgtcg ccaagctgac gataaacctg cgccagcgcc gttgcgccca
    62761 gcgcgttatt gcctttgccc aaatcaatca gcagcagtgc gttatcttcg gtagaaagct
    62821 gcggcgtgat ggtgtgacgt acatcttcca cgcgggcaaa tgcagaaatc accagcgaca
    62881 gcggcgacgt catttcgcgc tcttcgttac cttcctgcca gcgggttttc atcgacatag
    62941 agtctttacc caccgggatc gtcaggccca gcgccggaca aagctcttcg cccacggctt
    63001 taacggcttc atacaggccc gcatcttcac cagggtggcc tgccgccgcc atccagttgg
    63061 cggaaagttt aatgcgtttg atatcgccaa tctgtgttgc ggcaatattg gttaacgctt
    63121 caccgaccgc cagacgggca gaggcggcga aatccagcag cgcaaccggc gcacgctcgc
    63181 caatcgccat cgcttcaccg tagtagctgt cgaggctggc ggtagtgacc gcgcagttag
    63241 cgaccggcac ctgccacgga cccaccatct gatcgcgcgc taccatgcca gttacgctgc
    63301 ggtcaccgat ggtcaccagg aaagttttct ccgccacggt cggcagatgc agtacgcgtt
    63361 tcaccgcgtc tgcaatggtg atcccttcac ggaccagcgc atcgcccttc gctttcagcg
    63421 tttgtacatc gcgggtcatc ttcggcgttt taccaagcag gacgtccagc ggcagatcga
    63481 tcggctgatt atcaaaatga cgatcgtgca gagaaagatg cagttcttcg gtcgcttcac
    63541 caatcaccgc gtagggtgcg cgctcacgct tacacagttc gtcaaacagc ggtaattgat
    63601 cggcagcaac cgccagcacg tagcgttcct gggattcgtt acaccagatt tccagcgggc
    63661 tcatgcccgg ttcgtcgctt agaatatcgc gcagttcaaa tttaccgccg cgtccgccgt
    63721 cgctcaccag ctccggcatc gcgttagaaa gaccgcccgc accgacgtcg tggataaaca
    63781 gaattgggtt agcatcacca agctgccagc aacggtcgat cacttcctgg caacgacgct
    63841 ccatctccgg gttgtcgcgc tgtacggaag caaagtcgag gtcggcatca gactgacctg
    63901 acgccatgga agacgccgct ccgccgccaa gaccgatgtt cattgccggg ccgccgagaa
    63961 cgaccagctt cgcaccgacg ttgatctcgc ctttttgtac gtgatcggcg cgaatgttgc
    64021 cgatcccgcc cgccagcatg atcggtttgt gataaccgcg cagctcttcg ccgttgtggc
    64081 tgttcacttt ttcttcataa gtacggaagt agccattcag tgccggacga ccaaattcgt
    64141 tgttaaacgc cgcgccgccc agcgggcctt cggtcatgat gtccagcgcg gtgacaatgc
    64201 gctcaggctt accgaaatct tcttcccacg gctgttcgaa gccaggaatt cgcaggttgg
    64261 aaacggagaa accaaccaaa cccgctttcg gcttcgcgcc gaggccggtg gcaccttcat
    64321 cgcggatttc accgccggaa ccggtcgccg cgcccggcca cggagaaatc gccgtcgggt
    64381 ggttgtgagt ttcgactttc atcagaatat gcgccggttc ctgatggaaa tcgtagcggc
    64441 ccgtttcgtg gtcagcaaag tagcggccca cttcagaacc ttccattacg gcggcgttat
    64501 ctttataagc agagagaacg taatctggcg tggtttcgaa ggtgtttttg atcatcttaa
    64561 acagcgattt cggctgttgc tcaccgtcga taacccaatc ggcgttaaaa atcttgtggc
    64621 ggcagtgctc ggagttcgcc tgggcgaaca tatacagctc gatgtcgttc gggttacgac
    64681 caagctttgt gaaagcatcc tgcagatagt caatttcatc ttccgccagt gccaggccaa
    64741 gacgcaggtt agcgtcgatt agcgcctgac ggccctgccc cagcaaatca acgctggtga
    64801 ccggagtcgg ttgatggtgt gcaaacagct gctctgcatc atctaaagca aaaaagaccg
    64861 tttccatcat gcggtcgtgc agttcagcgg taacctgctg ccattgttca ttggtcagcg
    64921 taccggcttc tatatagtaa gcaacgccgc gctcaaggcg gtttacctgt tgtagcccgc
    64981 agttatgggc aatatcggtc gctttcgaag accagggaga gatggtgcca ggacgcgggg
    65041 tcaccagcag gagtttgcct tgcggggcgt ggctggcgag tgccgggcca tatttcagca
    65101 ggcgttcaag ttgtgcgtgc tcatcatcgt ttaacggcgc attgaggtca gcaaaatgga
    65161 catactcggc gtaaatattg tgaaccggga gcctggcagc ctgaaaacgt gccagcagtt
    65221 tgttgattcg gaatgccgac agtgcaggcg aaccacgcag aatttccatc ataagtctct
    65281 cgtcttctaa gctttcggtg tacccaaggg ggggaaacgg gcgtcattat aaagaatctg
    65341 atgcgctgac gaaaccgttt gcgtggaaat aaaatcacca tcgtgaatta gcaacgcgtg
    65401 ccgccaatgg ctgtaataag ttgccatctg gcgcaggttt acgcaaaatg ccgctcattt
    65461 aagagtaaac ctttcactat tattacgttt tttcaagctg ggacgcgcac ga
//
LOCUS       DAZKHF020000002        58533 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2201S31241
            SAMN43267800-rid25106413.denovo.002, whole genome shotgun sequence.
ACCESSION   DAZKHF020000002 DAZKHF020000000
VERSION     DAZKHF020000002.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267800
            Sequence Read Archive: SRR30307263
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 58533)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 58533)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 58533)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350581.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350581.2
            Genome Coverage       :: 151x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:21:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,652
            CDSs (total)                      :: 5,557
            Genes (coding)                    :: 5,320
            CDSs (with protein)               :: 5,320
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 237
            CDSs (without protein)            :: 237
            Pseudo Genes (ambiguous residues) :: 0 of 237
            Pseudo Genes (frameshifted)       :: 102 of 237
            Pseudo Genes (incomplete)         :: 130 of 237
            Pseudo Genes (internal stop)      :: 52 of 237
            Pseudo Genes (multiple problems)  :: 41 of 237
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..58533
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267800-rid25106413.denovo.002"
                     /serotype="O 26:H11"
                     /isolate="2201S31241"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-01"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            complement(<1..449)
                     /gene="folK"
                     /locus_tag="ACHOSC_RS00275"
                     /old_locus_tag="pgaptmp_000055"
     CDS             complement(<1..449)
                     /gene="folK"
                     /locus_tag="ACHOSC_RS00275"
                     /old_locus_tag="pgaptmp_000055"
                     /EC_number="2.7.6.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-amino-4-hydroxy-6-
                     hydroxymethyldihydropteridine diphosphokinase"
                     /protein_id="HGU4546072.1"
                     /translation="MTVAYIAIGSNLASPLEQVNAALKALGDIPESHILAVSSFYRTP
                     PLGPQDQPDYLNAAVALETSLAPEELLNHTQRIELQQGRVRKAERWGPRTLDLDIMLF
                     GNEVINTERLAVPHYDMKNRGFMLWPLFEIAPELAFPDGETLREVLHT"
     gene            complement(446..1843)
                     /gene="pcnB"
                     /locus_tag="ACHOSC_RS00280"
                     /old_locus_tag="pgaptmp_000056"
     CDS             complement(446..1843)
                     /gene="pcnB"
                     /locus_tag="ACHOSC_RS00280"
                     /old_locus_tag="pgaptmp_000056"
                     /EC_number="2.7.7.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414685.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:1990817 - poly(A) RNA polymerase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polynucleotide adenylyltransferase PcnB"
                     /protein_id="HGU4546073.1"
                     /translation="MFTRVANFCRKVLSREESEAEQAVARPQVTVIPREQHAISRKDI
                     SENALKVMYRLNKAGYEAWLVGGGVRDLLLGKKPKDFDVTTNATPEQVRKLFRNCRLV
                     GRRFRLAHVMFGPEIIEVATFRGHHEGNVSDRTTSQRGQNGMLLRDNIFGSIEEDAQR
                     RDFTINSLYYSVADFTVRDYVGGMKDLKDGVIRLIGNPETRYREDPVRMLRAVRFAAK
                     LGMRISPETAEPIPRLATLLNDIPPARLFEESLKLLQAGYGYDTYKLLCEYHLFQPLF
                     PTITRYFTENGDSPMERIIEQVLKNTDTRIHNDMRVNPAFLFAAMFWYPLLETAQKIA
                     QESGLTYHDAFALAMNDVLDEACRSLAIPKRLTTLTRDIWQLQLRMSRRQGKRAWKLL
                     EHPKFRAAYDLLALRAEVERNAELQRLVKWWGEFQVSAPPDQKGMLNELDEEPSPRRR
                     TRRPRKRAPRREGTA"
     gene            complement(1903..2799)
                     /gene="gluQRS"
                     /locus_tag="ACHOSC_RS00285"
                     /old_locus_tag="pgaptmp_000057"
     CDS             complement(1903..2799)
                     /gene="gluQRS"
                     /locus_tag="ACHOSC_RS00285"
                     /old_locus_tag="pgaptmp_000057"
                     /EC_number="6.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001432381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA];
                     GO_process: GO:0043039 - tRNA aminoacylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA glutamyl-Q(34) synthetase GluQRS"
                     /protein_id="HGU4546074.1"
                     /translation="MTDTQYIGRFAPSPSGELHFGSLIAALGSYLQARARQGRWLVRI
                     EDIDPPREVPGAAETILRQLEHYGLHWDGDVLWQSQRHHAYREALAWLHEQGLSYYCT
                     CTRARIQSIGGIYDGHCRVLHHGPDNAAVRIRQQHPVTQFTDQLRGIIHADEKLARED
                     FIIHRRDGLFAYNLAVVVDDHFQGVTEIVRGADLIEPTVRQISLYQLFGWKVPDYIHL
                     PLALNPQGAKLSKQNHAPALPKGDPRPVLIAALQFLGQQAEAHWQDFSVEQILQSAVK
                     NWRLTAVPESAIVNSTFSNASC"
     gene            complement(2866..3321)
                     /gene="dksA"
                     /locus_tag="ACHOSC_RS00290"
                     /old_locus_tag="pgaptmp_000058"
     CDS             complement(2866..3321)
                     /gene="dksA"
                     /locus_tag="ACHOSC_RS00290"
                     /old_locus_tag="pgaptmp_000058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002209354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase-binding protein DksA"
                     /protein_id="HGU4546075.1"
                     /translation="MQEGQNRKTSSLSILAIAGVEPYQEKPGEEYMNEAQLAHFRRIL
                     EAWRNQLRDEVDRTVTHMQDEAANFPDPVDRAAQEEEFSLELRNRDRERKLIKKIEKT
                     LKKVEDEDFGYCESCGVEIGIRRLEARPTADLCIDCKTLAEIREKQMAG"
     gene            complement(3499..4203)
                     /gene="sfsA"
                     /locus_tag="ACHOSC_RS00295"
                     /old_locus_tag="pgaptmp_000059"
     CDS             complement(3499..4203)
                     /gene="sfsA"
                     /locus_tag="ACHOSC_RS00295"
                     /old_locus_tag="pgaptmp_000059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA/RNA nuclease SfsA"
                     /protein_id="HGU4546076.1"
                     /translation="MEFSPPLQRATLIQRYKRFLADVITPDGRELTLHCPNTGAMTGC
                     ATPGDTVWYSTSDNTKRKYPHTWELTQSQSGAFICVNTLWANRLTKEAILNESISELS
                     GYSSLKSEVKYGAERSRIDFMLQADSRPDCYIEVKSVTLAENEQGYFPDAVTERGQKH
                     LRELMSVAAEGQRAVIFFAVLHSAITRFSPARHIDEKYAQLLSEAQQRGVEILAYKAE
                     ISAEGMALKKSLPVTL"
     gene            complement(4218..4748)
                     /gene="thpR"
                     /locus_tag="ACHOSC_RS00300"
                     /old_locus_tag="pgaptmp_000060"
     CDS             complement(4218..4748)
                     /gene="thpR"
                     /locus_tag="ACHOSC_RS00300"
                     /old_locus_tag="pgaptmp_000060"
                     /EC_number="3.1.4.58"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414689.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004113 - 2',3'-cyclic-nucleotide
                     3'-phosphodiesterase activity [Evidence IEA];
                     GO_function: GO:0008664 - RNA 2',3'-cyclic
                     3'-phosphodiesterase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA 2',3'-cyclic phosphodiesterase"
                     /protein_id="HGU4546077.1"
                     /translation="MSEPQRLFFAIDLPAEIREQIIHWRATHFPPEAGRPVAADNLHL
                     TLAFLGEVSAEKEKALSLLAGRIRQPGFTLTLDDAGQWLRSRVVWLGMRQPPRGLIQL
                     ANMLRSQAARSGCFQSNRPFHPHITLLRDASEAVTIPPPGFNWSYAVTEFTLYASSFA
                     RGRTRYTPLKRWALTQ"
     gene            4822..7251
                     /gene="hrpB"
                     /locus_tag="ACHOSC_RS00305"
                     /old_locus_tag="pgaptmp_000061"
     CDS             4822..7251
                     /gene="hrpB"
                     /locus_tag="ACHOSC_RS00305"
                     /old_locus_tag="pgaptmp_000061"
                     /EC_number="3.6.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414690.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent helicase HrpB"
                     /protein_id="HGU4546078.1"
                     /translation="MSSLPVAAVLPELLTALDCAPQVLLSAPTGAGKSTWLPLQLLAH
                     PGINGKIILLEPRRLAARNVAQRLAELLNEKPGDTVGYRMRAQNCVGPNTRLEVVTEG
                     VLTRMIQRDPELSGVGLVILDEFHERSLQADLALALLLDVQQGLRDDLKLLIMSATLD
                     NDRLQQMLPEAPVVISEGRSFPVERRYLPLPTHQRFDEAVAVATAEMLRQESGSLLLF
                     LPGVGEIQRVQEQLASRIGSDVLLCPLYGALSLNDQRKAILPAPQGMRKVVLATNIAE
                     TSLTIEGIRLVVDCAQERVARFDPRTGLTRLITQRVSQASMTQRAGRAGRLEPGICLH
                     LIAKEQAERAAAQSEPEILQSDLSGLLMELLQWGCSDPAQMSWLDQPPVVNLLAAKRL
                     LQMLGALEGERLSAQGQKMAALGNDPRLAAMLVSAKNDDEAATAAKIAAILEEPPRMG
                     NSDLGVAFSRNQPAWQQRSQQLLKRLNVRGGEADSSLIAPLLAGAFADRIARRRGQDG
                     RYQLANGMGAMLDANDALSRHEWLIAPLLLQGSASPDARILLALPVDIDELVQRCPQL
                     VQQSDTVEWDDAQGTLKAWRRLQIGLLTVKVQPLAKPSEDELHQAMLNGIRDKDLSVL
                     NWTAEAEQLRLRLLCAAKWLPEYDWPAVDDESLLATLETWLLPHMTGVHSLRGLKSLD
                     IYQALRGLLDWGMQQRLDSELPAHYTVPTGSRIAIRYHEDNPPALAVRMQEMFGEATN
                     PTIAQGRVPLVLELLSPAQRPLQITRDLSAFWKGAYREVQKEMKGRYPKHVWPDDPAN
                     TAPTRRTKKYS"
     gene            7383..9917
                     /gene="mrcB"
                     /locus_tag="ACHOSC_RS00310"
                     /old_locus_tag="pgaptmp_000062"
     CDS             7383..9917
                     /gene="mrcB"
                     /locus_tag="ACHOSC_RS00310"
                     /old_locus_tag="pgaptmp_000062"
                     /EC_number="2.4.99.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706097.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009274 - peptidoglycan-based cell wall
                     [Evidence IEA];
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA];
                     GO_function: GO:0008955 - peptidoglycan
                     glycosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0046677 - response to antibiotic [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional glycosyl
                     transferase/transpeptidase"
                     /protein_id="HGU4546079.1"
                     /translation="MAGNDREPIGRKGKPTRPVKQKVSRRRYEDDDDYDDYDDYEDEE
                     PMPRKGKGKGKGRKPRGKRGWLWLLLKLAIVFAVLIAIYGVYLDQKIRSRIDGKVWQL
                     PAAVYGRMVNLEPDMTISKNEMVKLLEATQYRQVSKMTRPGEFTVQANSIEMIRRPFD
                     FPDSKEGQVRARLTFDGDHLATIVNMENNRQFGFFRLDPRLITMISSPNGEQRLFVPR
                     SGFPDLLVDTLLATEDRHFYEHDGISLYSIGRAVLANLTAGRTVQGASTLTQQLVKNL
                     FLSSERSYWRKANEAYMALIMDARYSKDRILELYMNEVYLGQSGDNEIRGFPLASLYY
                     FGRPVEELSLDQQALLVGMVKGASIYNPWRNPKLALERRNLVLRLLQQQQIIDQELYD
                     MLSARPLGVQPRGGVISPQPAFMQLVRQELQAKLGDKVKDLSGVKIFTTFDSVAQDAA
                     EKAAVEGIPALKKQRKLSDLETAIVVVDRFSGEVRAMVGGSEPQFAGYNRAMQARRSI
                     GSLAKPATYLTALSQPKIYRLNTWIADAPIALRQPNGQVWSPQNDDRRYSESGRVMLV
                     DALTRSMNVPTVNLGMALGLPAVTETWIKLGVPKDQLHPVPAMLLGALNLTPIEVAQA
                     FQTIASGGNRAPLSALRSVIAEDGKVLYQSFPQAERAVPAQAAYLTLWTMQQVVQRGT
                     GRQLGAKYPNLHLAGKTGTTNNNVDTWFAGIDGSTVTITWVGRDNNQPTKLYGASGAM
                     SIYQRYLANQTPTPLNLVPPEDIADMGVDYDGNFVCSGGMRVLPVWTSDPQSLCQQSE
                     MQQQPSGNPFDQSSQPQQQPQQQPAQQEQKDSDGVAGWIKDMFGSN"
     gene            10137..12380
                     /gene="fhuA"
                     /locus_tag="ACHOSC_RS00315"
                     /old_locus_tag="pgaptmp_000063"
     CDS             10137..12380
                     /gene="fhuA"
                     /locus_tag="ACHOSC_RS00315"
                     /old_locus_tag="pgaptmp_000063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0015344 - siderophore uptake transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0038023 - signaling receptor activity
                     [Evidence IEA];
                     GO_process: GO:0015891 - siderophore transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferrichrome porin FhuA"
                     /protein_id="HGU4546080.1"
                     /translation="MARSKTAQPKHSLRKIAVVVATAVSGMSVYAQAAVEPKEDTITV
                     TVAPAPQESAWGPAATIAARQSATGTKTDTPIQKVPQSISVVTAEEMALHQPKSVKEA
                     LSYTPGVSVGTRGASNTYDHLIIRGFAAEGQSQNNYLNGLKLQGNFYNDAVIDPYMLE
                     RAEIMRGPVSVLYGKSSPGGLLNMVSKRPTTEPLKEVQFKAGTDSLFQTGFDFSDSLD
                     DDGVYSYRLTGLARSANAQQKGSEEQRYAIAPAFTWRPDDKTNFTFLSYFQNEPETGY
                     YGWLPKEGTVEPLPNGKRLPTDFNEGAKNNTYSRNEKMVGYSFDHEFNDTFTVRQNLR
                     FAENKTSQNSVYGYGVCSDPANAYSKQCAALAPADKGHYLARKYVVDDEKLQNFSVDT
                     QLQSKFATGDIDHTLLSGVDFMRMRNDINAWFGYDDSVPLLNLYNPVNTDFDFNAKDP
                     ANSGPYRILNKQKQTGVYVQDQAQWDKVLVTLGGRYDWADQESLNRVAGTTDKRDDKQ
                     FTWRGGVNYLFDNGVTPYFSYSESFEPSSQVGKDGNIFAPSKGKQYEVGVKYVPEDRP
                     IVVTGAVYNLTKTNNLMADPEGSFFSVEGGEIRARGVEIEAKAALSASVNVVGSYTYT
                     DAEYTTDTTYKGNTPAQVPKHMASLWADYTFFDGPLSGLTLGTGGRYTGSSYGDPANS
                     FKVGSYTVVDALVRYDLARVGMAGSNVALHVNNLFDREYVASCFNTYGCFWGAERQVV
                     ATATFRF"
     gene            12431..13228
                     /gene="fhuC"
                     /locus_tag="ACHOSC_RS00320"
                     /old_locus_tag="pgaptmp_000064"
     CDS             12431..13228
                     /gene="fhuC"
                     /locus_tag="ACHOSC_RS00320"
                     /old_locus_tag="pgaptmp_000064"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe3+-hydroxamate ABC transporter ATP-binding
                     protein FhuC"
                     /protein_id="HGU4546081.1"
                     /translation="MQEYTNHSDTTFALRNISFRVPGRTLLHPLSLTFPAGKVTGLIG
                     HNGSGKSTLLKMLGRHQPPSEGEILLDAQPLESWSSKAFARKVAYLPQQLPPAEGMTV
                     RELVAIGRYPWHGALGRFGAADREKVEEAISLVGLKPLAHRLVDSLSGGERQRAWIAM
                     LVAQDSRCLLLDEPTSALDIAHQVDVLALVHRLSQERGLTVIAVLHDINMAARYCDYL
                     VALRGGEMIAQGTPAEIMRGETLEMIYGIPMGILPHPAGAAPVSFVY"
     gene            13228..14118
                     /gene="fhuD"
                     /locus_tag="ACHOSC_RS00325"
                     /old_locus_tag="pgaptmp_000065"
     CDS             13228..14118
                     /gene="fhuD"
                     /locus_tag="ACHOSC_RS00325"
                     /old_locus_tag="pgaptmp_000065"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe(3+)-hydroxamate ABC transporter
                     substrate-binding protein FhuD"
                     /protein_id="HGU4546082.1"
                     /translation="MSGLPLISRRRLLTAMALSPLLWQMNTAHAAAIDPNRIVALEWL
                     PVELLLALGIVPYGVADTINYRLWVSEPPLPDSVIDVGLRTEPNLELLTEMKPSFMVW
                     SAGYGPSPEMLARIAPGRGFNFSDGKQPLAMARKSLTEMADLLNLQSAAETHLAHYED
                     FIRSMKPRFVKRGARPLLLTTLIDPRHMLVFGPNSLFQEILDEYGIPNAWQGETNFWG
                     STAVSIDRLAAYKDVDVLCFDHDNSKDMDALMATPLWQAMPFVRTGRFQRVPAVWFYG
                     ATLSAMHFVRVLDNAIGGKA"
     gene            14115..16097
                     /gene="fhuB"
                     /locus_tag="ACHOSC_RS00330"
                     /old_locus_tag="pgaptmp_000066"
     CDS             14115..16097
                     /gene="fhuB"
                     /locus_tag="ACHOSC_RS00330"
                     /old_locus_tag="pgaptmp_000066"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe(3+)-hydroxamate ABC transporter permease
                     FhuB"
                     /protein_id="HGU4546083.1"
                     /translation="MSKRIALFPALLLALLVIVATALTWMNFSQALPRSQWAQAAWSP
                     DIDVIEQMIFHYSLLPRLAISLLVGAGLGLVGVLFQQVLRNPLAEPTTLGVATGAQLG
                     ITVTTLWAIPGAMASQFAALVGACVVGLIVFGVAWGKRLSPVTLILAGLVVSLYCGAI
                     NQLLVIFHHDQLQSMFLWSTGTLTQTDWGGVERLWPQLLGGVMLTLLLLRPLTLMGLD
                     DGVARNLGLALSLARLAALSLAIVISALLVNAVGIIGFIGLFAPLLAKMLGARRLLPR
                     LMLASLIGALILWLSDQIILWLTRVWMEVSTGSVTALIGAPLLLWLLPRLRSISAPDM
                     KVNDRVAAERQHVLAFALAGGVLLLMAVVVALSFGRDAHGWTWASGALLEDLMPWRWP
                     RIMAALFAGVMLAVAGCIIQRLTGNPMASPEVLGISSGAAFGVVLMLFLVPGNAFGWL
                     LPAGSLGAAVTLLIIMIAAGRGGFSPHRMLLAGMALSTAFTMLLMMLQASGDPRMAQV
                     LTWISGSTYNATDAQVWRTGIVMVILLAITPLCRRWLTILPLGGDTARAVGMALTPTR
                     IALLLLAACLTATATMTIGPLSFVGLMAPHIARMMGFRRTMPHIVISALVGGLLLVFA
                     DWCGRMVLFPFQIPAGLLSTFIGAPYFIYLLRKQSR"
     gene            complement(16132..17412)
                     /gene="hemL"
                     /locus_tag="ACHOSC_RS00335"
                     /old_locus_tag="pgaptmp_000067"
     CDS             complement(16132..17412)
                     /gene="hemL"
                     /locus_tag="ACHOSC_RS00335"
                     /old_locus_tag="pgaptmp_000067"
                     /EC_number="5.4.3.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020233394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042286 - glutamate-1-semialdehyde
                     2,1-aminomutase activity [Evidence IEA];
                     GO_process: GO:0033014 - tetrapyrrole biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-1-semialdehyde 2,1-aminomutase"
                     /protein_id="HGU4546084.1"
                     /translation="MSKSENLYSAARELIPGGVNSPVRAFTGVGGTPLFIEKADGAYL
                     YDVDGKAYIDYVGSWGPMVLGHNHPAIRNAVIEAAERGLSFGAPTEMEVKMAQLVTEL
                     VPTMDMVRMVNSGTEATMSAIRLARGFTGRDKIIKFEGCYHGHADCLLVKAGSGALTL
                     GQPNSPGVPADFAKHTLTCTYNDLASVRAAFEQYPQEIACIIVEPVAGNMNCVPPLPE
                     FLPGLRALCDEFGALLIIDEVMTGFRVALAGAQDYYGVEPDLTCLGKIIGGGMPVGAF
                     GGRRDVMDALAPTGPVYQAGTLSGNPIAMAAGFACLNEVAQPGVHETLDELTTRLAEG
                     LLEAAEEAGIPLVVNHVGGMFGIFFTDAESVTCYQDVMACDVERFKRFFHMMLDEGVY
                     LAPSAFEAGFMSVAHSMEDINNTIDAARRVFAKL"
     gene            17578..17625
                     /gene="yadX"
                     /locus_tag="ACHOSC_RS00340"
                     /old_locus_tag="pgaptmp_000068"
     CDS             17578..17625
                     /gene="yadX"
                     /locus_tag="ACHOSC_RS00340"
                     /old_locus_tag="pgaptmp_000068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YadX"
                     /protein_id="HGU4546085.1"
                     /translation="MERASKMPSSYLYDQ"
     gene            17637..19058
                     /gene="clcA"
                     /locus_tag="ACHOSC_RS00345"
                     /old_locus_tag="pgaptmp_000069"
     CDS             17637..19058
                     /gene="clcA"
                     /locus_tag="ACHOSC_RS00345"
                     /old_locus_tag="pgaptmp_000069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005247 - voltage-gated chloride channel
                     activity [Evidence IEA];
                     GO_process: GO:0006821 - chloride transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="H(+)/Cl(-) exchange transporter ClcA"
                     /protein_id="HGU4546086.1"
                     /translation="MKTDTPSLETPQAARLRRRQLIRQLLERDKTPLAILFMAAVVGT
                     LVGLAAVAFDKGVAWLQNQRMGALVHTADNYPLLLTVAFLCSAVLAMFGYFLVRKYAP
                     EAGGSGIPEIEGALEDQRPVRWWRVLPVKFFGGLGTLGGGMVLGREGPTVQIGGNIGR
                     MVLDIFRLKGDGARHTLLATGAAAGLAAAFNAPLAGILFIIEEMRPQFRYTLISIKAV
                     FIGVIMSTIMYRIFNHEVALIDVGKLSDAPLNTLWLYLILGIIFGIFGPIFNKWVLGM
                     QDLLHRVHGGNITKWVLMGGAIGGLCGLLGFVAPATSGGGFNLIPIATAGNFSMGMLV
                     FIFVARVITTLLCFSSGAPGGIFAPMLALGTVLGTAFGMVAVELFPQYHLEAGTFAIA
                     GMGALLAASIRAPLTGIILVLEMTDNYQLILPMIITGLGATLLAQFTGGKPLYSAILA
                     RTLAKQEAMQLARSKAASASENT"
     gene            19082..19147
                     /gene="yadW"
                     /locus_tag="ACHOSC_RS00350"
                     /old_locus_tag="pgaptmp_000070"
     CDS             19082..19147
                     /gene="yadW"
                     /locus_tag="ACHOSC_RS00350"
                     /old_locus_tag="pgaptmp_000070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="small protein YadW"
                     /protein_id="HGU4546087.1"
                     /translation="MAIIIGLEFAQLPMSFGAKYE"
     gene            19140..19484
                     /gene="erpA"
                     /locus_tag="ACHOSC_RS00355"
                     /old_locus_tag="pgaptmp_000071"
     CDS             19140..19484
                     /gene="erpA"
                     /locus_tag="ACHOSC_RS00355"
                     /old_locus_tag="pgaptmp_000071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001295564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster insertion protein ErpA"
                     /protein_id="HGU4546088.1"
                     /translation="MSDDVALPLEFTDAAANKVKSLIADEDNPNLKLRVYITGGGCSG
                     FQYGFTFDDQVNEGDMTIEKQGVGLVVDPMSLQYLVGGSVDYTEGLEGSRFIVTNPNA
                     KSTCGCGSSFSI"
     gene            complement(19531..20154)
                     /gene="yadS"
                     /locus_tag="ACHOSC_RS00360"
                     /old_locus_tag="pgaptmp_000072"
     CDS             complement(19531..20154)
                     /gene="yadS"
                     /locus_tag="ACHOSC_RS00360"
                     /old_locus_tag="pgaptmp_000072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRIC cation channel family protein"
                     /protein_id="HGU4546089.1"
                     /translation="MLVYWLDIVGTAVFAISGVLLAGKLRMDPFGVLVLGVVTAVGGG
                     TIRDMALDHGPVFWVKDPTDLVVAMVTSMLTIVLVRQPRRLPKWMLPVLDAVGLAVFV
                     GIGVNKAFNAEAGPLIAVCMGVITGVGGGIIRDVLAREIPMILRTEIYATACIIGGIV
                     HATAYYTFSVPLETASMMGMVVTLLIRLAAIRWHLKLPTFALDENGR"
     gene            complement(20192..20992)
                     /gene="btuF"
                     /locus_tag="ACHOSC_RS00365"
                     /old_locus_tag="pgaptmp_000073"
     CDS             complement(20192..20992)
                     /gene="btuF"
                     /locus_tag="ACHOSC_RS00365"
                     /old_locus_tag="pgaptmp_000073"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0031419 - cobalamin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="vitamin B12 ABC transporter substrate-binding
                     protein BtuF"
                     /protein_id="HGU4546090.1"
                     /translation="MAKSLFRALVALSFLAPLWLNAAPRVITLSPANTELAFAAGITP
                     VGVSSYSDYPPQAQKIEQVSTWQGMNLERIVALKPDLVIAWRGGNAERQVDQLASLGI
                     KVMWVDATSIEQIANALRQLAPWSPQPDKAEQAAQSLLDQYAQLKAQYADKPKKRVFL
                     QFGINPPFTSGKESIQNQVLEVCGGENIFKDSRVPWPQVSREQVLARSPQAIVITGGP
                     DQIPKIKQYWGEQLKIPVIPLTSDWFERASPRIILAAQQLCNALSQVD"
     gene            complement(20985..21683)
                     /gene="mtnN"
                     /locus_tag="ACHOSC_RS00370"
                     /old_locus_tag="pgaptmp_000074"
     CDS             complement(20985..21683)
                     /gene="mtnN"
                     /locus_tag="ACHOSC_RS00370"
                     /old_locus_tag="pgaptmp_000074"
                     /EC_number="3.2.2.16"
                     /EC_number="3.2.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008782 - adenosylhomocysteine
                     nucleosidase activity [Evidence IEA];
                     GO_function: GO:0008930 - methylthioadenosine nucleosidase
                     activity [Evidence IEA];
                     GO_process: GO:0009164 - nucleoside catabolic process
                     [Evidence IEA];
                     GO_process: GO:0019509 - L-methionine salvage from
                     methylthioadenosine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5'-methylthioadenosine/S-adenosylhomocysteine
                     nucleosidase"
                     /protein_id="HGU4546091.1"
                     /translation="MKIGIIGAMEEEVTLLRDKIEKRQTISLGGCEIYTGQLNGTEVA
                     LLKSGIGKVAAALGATLLLEHCKPDVIINTGSAGGLAPTLKVGDIVVSDEARYHDADV
                     TAFGYEYGQLPGCPAGFKADDKLIAAAEACIAELNLNAVRGLIVSGDAFINGSVGLAK
                     IRHNFPQAIAVEMEATAIAHVCHNFNVPFVVVRAISDVADQQSHLSFDEFLAVAAKQS
                     SLMVESLVQKLAHG"
     gene            21767..23284
                     /gene="dgt"
                     /locus_tag="ACHOSC_RS00375"
                     /old_locus_tag="pgaptmp_000075"
     CDS             21767..23284
                     /gene="dgt"
                     /locus_tag="ACHOSC_RS00375"
                     /old_locus_tag="pgaptmp_000075"
                     /EC_number="3.1.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008832 - dGTPase activity [Evidence IEA];
                     GO_process: GO:0006203 - dGTP catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dGTPase"
                     /protein_id="HGU4546092.1"
                     /translation="MAQIDFRKKINWHRRYRSPQGVKTEHEILRIFESDRGRIINSPA
                     IRRLQQKTQVFPLERNAAVRTRLTHSMEVQQVGRYIAKEILSRLKELKLLEAYGLDEL
                     TGPFESIVEMSCLMHDIGNPPFGHFGEAAINDWFRQRLHPEDAESQPLTDDRCSVAAL
                     RLRDGEEPLNELRRKIRQDLCHFEGNAQGIRLVHTLMRMNLTWAQVGGILKYTRPAWW
                     RGETPETHHYLMKKPGYYLSEEAYIARLRKELNLALYSRFPLTWIMEAADDISYCVAD
                     LEDAVEKRIFTVEQLYHHLHEAWGQHEKGSLFSLVVENAWEKSRSNSLSRSTEDQFFM
                     YLRVNTLNKLVPYAAQRFIDNLPAIFAGTFNHALLEDASECSDLLKLYKNVAVKHVFS
                     HPDVEQLELQGYRVISGLLEIYRPLLSLSLSDFTELVEKERVKRFPIESRLFHKLSTR
                     HRLAYVEAVSKLPSDSPEFPLWEYYYRCRLLQDYISGMTDLYAWDEYRRLMAVEQ"
     gene            23414..24838
                     /gene="degP"
                     /locus_tag="ACHOSC_RS00380"
                     /old_locus_tag="pgaptmp_000076"
     CDS             23414..24838
                     /gene="degP"
                     /locus_tag="ACHOSC_RS00380"
                     /old_locus_tag="pgaptmp_000076"
                     /EC_number="3.4.21.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine endoprotease DegP"
                     /protein_id="HGU4546093.1"
                     /translation="MKKTTLALSALALSLGLALSPLSATAAETSSATTAQQMPSLAPM
                     LEKVMPSVVSINVEGSTTVNTPRMPRNFQQFFGDDSPFCQEGSPFQSSPFCQGGQGGN
                     GGGQQQKFMALGSGVIIDADKGYVVTNNHVVDNATVIKVQLGDGRKFDAKMVGKDPRS
                     DIALIQIQNPKNLTAIKMADSDALRVGDYTVAIGNPFGLGETVTSGIVSALGRSGLNA
                     ENYENFIQTDAAINRGNSGGALVNLNGELIGINTAILAPDGGNIGIGFAIPSNMVKNL
                     TSQMVEYGQVKRGELGIMGTELNSELAKAMKVDAQRGAFVSQVLPNSSAAKAGIKAGD
                     VITSLNGKPISSFAALRAQVGTMPVGSKLTLGLLRDGKQVNVNLELQQSSQNQVDSSS
                     IFNGIEGAEMSNKGKDQGVVVNNVKTGTPAAQIGLKKGDVIIGANQQAVKNIAELRKV
                     LDSKPSVLALNIQRGDSSIYLLMQ"
     gene            24995..26146
                     /gene="cdaR"
                     /locus_tag="ACHOSC_RS00385"
                     /old_locus_tag="pgaptmp_000077"
                     /pseudo
     CDS             24995..26146
                     /gene="cdaR"
                     /locus_tag="ACHOSC_RS00385"
                     /old_locus_tag="pgaptmp_000077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308193.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator CdaR"
     gene            complement(26235..26621)
                     /gene="yaeH"
                     /locus_tag="ACHOSC_RS00390"
                     /old_locus_tag="pgaptmp_000078"
     CDS             complement(26235..26621)
                     /gene="yaeH"
                     /locus_tag="ACHOSC_RS00390"
                     /old_locus_tag="pgaptmp_000078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000272186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3461 family protein"
                     /protein_id="HGU4546094.1"
                     /translation="MYDNLKSLGITNPEEIDRYSLRQEANNDILKIYFQKDKGEFFAK
                     SVKFKYPRQRKTVVADGVGQGYKEVQEISPNLRYIIDELDQICQRDRSEVDLKRKILD
                     DLRHLESVVTNKISEIEADLEKLTRK"
     gene            complement(26783..27595)
                     /gene="yaeI"
                     /locus_tag="ACHOSC_RS00395"
                     /old_locus_tag="pgaptmp_000079"
     CDS             complement(26783..27595)
                     /gene="yaeI"
                     /locus_tag="ACHOSC_RS00395"
                     /old_locus_tag="pgaptmp_000079"
                     /EC_number="3.1.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414706.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphodiesterase YaeI"
                     /protein_id="HGU4546095.1"
                     /translation="MISRRRLLQATAATIATSSGFGYMHYWEPGWFELIRHRLAFFKD
                     NAAPFKILFLADLHYSRFVPLSLISDAIALGIEQKPDLILLGGDYVLFDMPLNFSAFS
                     DVLSPLAECAPTFACFGNHDRPVGTEKNHLIGETLKSAGITVLFNQATVIATPNRQFE
                     LVGTGDLWAGQCKPPPASEANLPRLVLAHNPDSKEVMRDEPWDLMLCGHTHGGQLRVP
                     LVGEPFAPVEDKRYVAGLNAFGERHIYTTRGVGSLYGLRLNCRPEVTMLELV"
     gene            complement(27650..28474)
                     /gene="dapD"
                     /locus_tag="ACHOSC_RS00400"
                     /old_locus_tag="pgaptmp_000080"
     CDS             complement(27650..28474)
                     /gene="dapD"
                     /locus_tag="ACHOSC_RS00400"
                     /old_locus_tag="pgaptmp_000080"
                     /EC_number="2.3.1.117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008666 -
                     2,3,4,5-tetrahydropyridine-2,6-dicarboxylate
                     N-succinyltransferase activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2,3,4,5-tetrahydropyridine-2,6-dicarboxylate
                     N-succinyltransferase"
                     /protein_id="HGU4546096.1"
                     /translation="MQQLQNIIETAFERRAEITPANADTVTREAVNQVIALLDSGALR
                     VAEKIDGQWVTHQWLKKAVLLSFRINDNQVIEGAESRYFDKVPMKFADYDEARFQKEG
                     FRVVPPAAVRQGAFIARNTVLMPSYVNIGAYVDEGTMVDTWATVGSCAQIGKNVHLSG
                     GVGIGGVLEPLQANPTIIEDNCFIGARSEVVEGVIVEEGSVISMGVYIGQSTRIYDRE
                     TGEIHYGRVPAGSVVVSGNLPSKDGKYSLYCAVIVKKVDAKTRGKVGINELLRTID"
     gene            complement(28505..31177)
                     /gene="glnD"
                     /locus_tag="ACHOSC_RS00405"
                     /old_locus_tag="pgaptmp_000081"
     CDS             complement(28505..31177)
                     /gene="glnD"
                     /locus_tag="ACHOSC_RS00405"
                     /old_locus_tag="pgaptmp_000081"
                     /EC_number="2.7.7.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     uridylyltransferase/uridylyl-removing protein GlnD"
                     /protein_id="HGU4546097.1"
                     /translation="MNTLPEQYANTALPTLPGQLQNPCVWPRDELTVGGIKAHIDTFQ
                     RWLGDAFDNGISAEQLIEARTEFIDQLLQRLWIEAGFSQIADLALVAVGGYGRGELHP
                     LSDIDLLILSRKKLPDDQAQKVGELLTLLWDVKLEVGHSVRTLEECMLEGLSDLTVAT
                     NLIESRLLIGDVALFLELQKHIFSEGFWPSDKFYAAKVEEQNQRHQRYHGTSYNLEPD
                     IKSSPGGLRDIHTLQWVARRHFGATSLDEMVGFGFLTSAERAELNECLHILWRIRFAL
                     HLVVSRYDNRLLFDRQLSVAQRLNYSGEGNEPVERMMKDYFRVTRRVSELNQMLLQLF
                     DEAILALPADEKPRPLDDEFQLRGTLIDLRDETLFMRQPEAILRMFYTMVRNSAITGI
                     YSTTLRQLRHARRHLQQPLCNIPEARKLFLSILRHPGAVRRGLLPMHRHSVLGAYMPQ
                     WSHIVGQMQFDLFHAYTVDEHTIRVMLKLESFASEETRQRHPLCVDVWPRLPSTELIF
                     IAALFHDIAKGRGGDHSILGAQDVVHFAELHGLNSRETQLVAWLVRQHLLMSVTAQRR
                     DIQDPEVIKQFAEEVQTENRLRYLVCLTVADICATNETLWNSWKQSLLRELYFATEKQ
                     LRRGMQNTPDMRERVRHHQLQALALLRMDNIDEEALHQIWSRCRANYFVRHSPNQLAW
                     HARHLLQHDLSKPLVLLSPQATRGGTEIFIWSPDRPYLFAAVCAELDRRNLSVHDAQI
                     FTTRDGMAMDTFIVLEPDGSPLSADRHEVIRFGLEQVLTQSSWQPPQPRRQPAKLRHF
                     TVETEVTFLPTHTDRKSFLELIALDQPGLLARVGKIFADLGISLHGARITTIGERVED
                     LFIIATADRRALNNELQQEVHQRLTEALNPNDKG"
     gene            complement(31239..32033)
                     /gene="map"
                     /locus_tag="ACHOSC_RS00410"
                     /old_locus_tag="pgaptmp_000082"
     CDS             complement(31239..32033)
                     /gene="map"
                     /locus_tag="ACHOSC_RS00410"
                     /old_locus_tag="pgaptmp_000082"
                     /EC_number="3.4.11.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070006 - metalloaminopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I methionyl aminopeptidase"
                     /protein_id="HGU4546098.1"
                     /translation="MAISIKTPEDIEKMRVAGRLAAEVLEMIEPYVKPGVSTGELDRI
                     CNDYIVNEQHTVSACLGYHGYPKSVCISINEVVCHGIPDDAKLLKDGDIVNIDVTVIK
                     DGFHGDTSKMFIVGKPTIMGERLCRITQESLYLALRMVKPGINLREIGAAIQKFVEAE
                     GFSVVREYCGHGIGRGFHEEPQVLHYDSRETNVVLKPGMTFTIEPMVNAGKKEIRTMK
                     DGWTVKTKDRSLSAQYEHTIVVTDNGCEILTLRKDDTIPAIISHDE"
     gene            32401..33126
                     /gene="rpsB"
                     /locus_tag="ACHOSC_RS00415"
                     /old_locus_tag="pgaptmp_000083"
     CDS             32401..33126
                     /gene="rpsB"
                     /locus_tag="ACHOSC_RS00415"
                     /old_locus_tag="pgaptmp_000083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S2"
                     /protein_id="HGU4546099.1"
                     /translation="MATVSMRDMLKAGVHFGHQTRYWNPKMKPFIFGARNKVHIINLE
                     KTVPMFNEALAELNKIASRKGKILFVGTKRAASEAVKDAALSCDQFFVNHRWLGGMLT
                     NWKTVRQSIKRLKDLETQSQDGTFEKLTKKEALMRTRELEKLENSLGGIKDMGGLPDA
                     LFVIDADHEHIAIKEANNLGIPVFAIVDTNSDPDGVDFVIPGNDDAIRAVTLYLGAVA
                     ATVREGRSQDLASQAEESFVEAE"
     gene            33384..34235
                     /gene="tsf"
                     /locus_tag="ACHOSC_RS00420"
                     /old_locus_tag="pgaptmp_000084"
     CDS             33384..34235
                     /gene="tsf"
                     /locus_tag="ACHOSC_RS00420"
                     /old_locus_tag="pgaptmp_000084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation elongation factor Ts"
                     /protein_id="HGU4546100.1"
                     /translation="MAEITASLVKELRERTGAGMMDCKKALTEANGDIELAIENMRKS
                     GAIKAAKKAGNVAADGVIKTKIDGNYGIILEVNCQTDFVAKDAGFQAFADKVLDAAVA
                     GKITDVEVLKAQFEEERVALVAKIGENINIRRVAALEGDVLGSYQHGARIGVLVAAKG
                     ADEELVKHIAMHVAASKPEFIKPEDVSAEVVEKEYQVQLDIAMQSGKPKEIAEKMVEG
                     RMKKFTGEVSLTGQPFVMEPSKTVGQLLKEHNAEVTGFIRFEVGEGIEKVETDFAAEV
                     AAMSKQS"
     gene            34382..35107
                     /gene="pyrH"
                     /locus_tag="ACHOSC_RS00425"
                     /old_locus_tag="pgaptmp_000085"
     CDS             34382..35107
                     /gene="pyrH"
                     /locus_tag="ACHOSC_RS00425"
                     /old_locus_tag="pgaptmp_000085"
                     /EC_number="2.7.4.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000224576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0033862 - UMP kinase activity [Evidence
                     IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UMP kinase"
                     /protein_id="HGU4546101.1"
                     /translation="MATNAKPVYKRILLKLSGEALQGTEGFGIDASILDRMAQEIKEL
                     VELGIQVGVVIGGGNLFRGAGLAKAGMNRVVGDHMGMLATVMNGLAMRDALHRAYVNA
                     RLMSAIPLNGVCDSYSWAEAISLLRNNRVVILSAGTGNPFFTTDSAACLRGIEIEADV
                     VLKATKVDGVFTADPAKDPTATMYEQLTYSEVLEKELKVMDLAAFTLARDHKLPIRVF
                     NMNKPGALRRVVMGEKEGTLITE"
     gene            35399..35956
                     /gene="frr"
                     /locus_tag="ACHOSC_RS00430"
                     /old_locus_tag="pgaptmp_000086"
     CDS             35399..35956
                     /gene="frr"
                     /locus_tag="ACHOSC_RS00430"
                     /old_locus_tag="pgaptmp_000086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000622421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008135 - translation factor activity, RNA
                     binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome recycling factor"
                     /protein_id="HGU4546102.1"
                     /translation="MISDIRKDAEVRMDKCVEAFKTQISKIRTGRASPSLLDGIVVEY
                     YGTPTPLRQLASVTVEDSRTLKINVFDRSMSPAVEKAIMASDLGLNPNSAGSDIRVPL
                     PPLTEERRKDLTKIVRGEAEQARVAVRNVRRDANDKVKALLKDKEISEDDDRRSQDDV
                     QKLTDAAIKKIEAALADKEAELMQF"
     gene            36048..37244
                     /gene="ispC"
                     /locus_tag="ACHOSC_RS00435"
                     /old_locus_tag="pgaptmp_000087"
     CDS             36048..37244
                     /gene="ispC"
                     /locus_tag="ACHOSC_RS00435"
                     /old_locus_tag="pgaptmp_000087"
                     /EC_number="1.1.1.267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0030604 - 1-deoxy-D-xylulose-5-phosphate
                     reductoisomerase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0070402 - NADPH binding [Evidence IEA];
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate reductoisomerase"
                     /protein_id="HGU4546103.1"
                     /translation="MKQLTILGSTGSIGCSTLDVVRHNPEHFRVVALVAGKNVTRMVE
                     QCLEFSPRYAVMDDEASAKLLKTMLQQQGSRTEVLSGQQAACDMAALEDVDQVMAAIV
                     GAAGLLPTLAAIRAGKTILLANKESLVTCGRLFMDAVKQSKAQLLPVDSEHNAIFQSL
                     PQPIQHNLGYADLEQNGVVSILLTGSGGPFRETPLRDLATMTPDQACRHPNWSMGRKI
                     SVDSATMMNKGLEYIEARWLFNASASQMEVLIHPQSVIHSMVRYQDGSVLAQLGEPDM
                     RTPIAHTMAWPNRVNSGVKPLDFCKLSALTFAAPDYDRYPCLKLAMEAFEQGQAATTA
                     LNAANEITVAAFLAQQIRFTDIAALNLSVLEKMDMREPQCVDDVLSVDANAREVARKE
                     VMRLAS"
     gene            37433..38191
                     /gene="ispU"
                     /locus_tag="ACHOSC_RS00440"
                     /old_locus_tag="pgaptmp_000088"
                     /gene_synonym="uppS"
     CDS             37433..38191
                     /gene="ispU"
                     /locus_tag="ACHOSC_RS00440"
                     /old_locus_tag="pgaptmp_000088"
                     /gene_synonym="uppS"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308203.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0002094 - polyprenyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008834 -
                     di-trans,poly-cis-undecaprenyl-diphosphate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0043164 - Gram-negative-bacterium-type cell
                     wall biogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(2E,6E)-farnesyl-diphosphate-specific
                     ditrans,polycis-undecaprenyl-diphosphate synthase"
                     /protein_id="HGU4546104.1"
                     /translation="MLSATQPLSEKLPAHGCRHVAIIMDGNGRWAKKQGKIRAFGHKA
                     GAKSVRRAVSFAANNGIEALTLYAFSSENWNRPAQEVSALMELFVWALDSEVKSLHRH
                     NVRLRIIGDTSRFNSRLQERIRKSEALTAGNSGLTLNIAANYGGRWDIVQGVRQLAEK
                     VQQGNLQPDQIDEEMLNQHVCMHELAPVDLVIRTGGEHRISNFLLWQIAYAELYFTDV
                     LWPDFDEQDFEGALNAFANRERRFGGTEPGDETA"
     gene            38204..39061
                     /gene="cdsA"
                     /locus_tag="ACHOSC_RS00445"
                     /old_locus_tag="pgaptmp_000089"
     CDS             38204..39061
                     /gene="cdsA"
                     /locus_tag="ACHOSC_RS00445"
                     /old_locus_tag="pgaptmp_000089"
                     /EC_number="2.7.7.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414717.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016772 - transferase activity,
                     transferring phosphorus-containing groups [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidate cytidylyltransferase"
                     /protein_id="HGU4546105.1"
                     /translation="MLKYRLISAFVLIPVVIAALFLLPPVGFAIVTLVVCMLAAWEWG
                     QLSGFTTRSQRVWLAVLCGLLLALMLFLLPEYHRNIHQPLVEISLWASLGWWIVALLL
                     VLFYPGSAAIWRNSKTLRLIFGVLTIVPFFWGMLALRAWHYDENHYSGAIWLLYVMIL
                     VWGADSGAYMFGKLFGKHKLAPKVSPGKTWQGFIGGLATAAVISWGYGMWANLDVAPV
                     TLLICSIVAALASVLGDLTESMFKREAGIKDSGHLIPGHGGILDRIDSLTAAVPVFAC
                     LLLLVFRTL"
     gene            39073..40425
                     /gene="rseP"
                     /locus_tag="ACHOSC_RS00450"
                     /old_locus_tag="pgaptmp_000090"
     CDS             39073..40425
                     /gene="rseP"
                     /locus_tag="ACHOSC_RS00450"
                     /old_locus_tag="pgaptmp_000090"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma E protease regulator RseP"
                     /protein_id="HGU4546106.1"
                     /translation="MLSFLWDLASFIVALGVLITVHEFGHFWVARRCGVRVERFSIGF
                     GKALWRRTDKLGTEYVIALIPLGGYVKMLDERAEPVVPELRHHAFNNKSVGQRAAIIA
                     AGPVANFIFAIFAYWLVFIIGVPGVRPVVGEIAANSIAAEAQIAPGTELKAVDGIETP
                     DWDAVRLQLVDKIGDESTTITVAPFGSDQRRDVKLDLRHWAFEPDKEDPVSSLGIRPR
                     GPQIEPVLENVQPNSAASKAGLQAGDRIVKVDGQPLTQWVTFVMLVRDNPGKSLALEI
                     ERQGSPLSLTLIPESKPGNGKAIGFVGIEPKVIPLPDEYKVVRQYGPFNAIVEATDKT
                     WQLMKLTVSMLGKLITGDVKLNNLSGPISIAKGAGMTAELGVVYYLPFLALISVNLGI
                     INLFPLPVLDGGHLLFLAIEKIKGGPVSERVQDFCYRIGSILLVLLMGLALFNDFSRL
                     "
     gene            40455..42887
                     /gene="bamA"
                     /locus_tag="ACHOSC_RS00455"
                     /old_locus_tag="pgaptmp_000091"
     CDS             40455..42887
                     /gene="bamA"
                     /locus_tag="ACHOSC_RS00455"
                     /old_locus_tag="pgaptmp_000091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_process: GO:0071709 - membrane assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamA"
                     /protein_id="HGU4546107.1"
                     /translation="MAMKKLLIASLLFSSATVYGAEGFVVKDIHFEGLQRVAVGAALL
                     SMPVRTGDTVNDEDISNTIRALFATGNFEDVRVLRDGDTLLVQVKERPTIASITFSGN
                     KSVKDDMLKQNLEASGVRVGESLDRTTIADIEKGLEDFYYSVGKYSASVKAVVTPLPR
                     NRVDLKLVFQEGVSAEIQQINIVGNHAFTTDELISHFQLRDEVPWWNVVGDRKYQKQK
                     LAGDLETLRSYYLDRGYARFNIDSTQVSLTPDKKGIYVTVNITEGDQYKLSGVEVSGN
                     LAGHSAEIEQLTKIEPGEQYNGTKVTKMEDDIKKLLGRYGYAYPRVQSMPEINDADKT
                     VKLRVNVDAGNRFYVRKIRFEGNDTSKDAVLRREMRQMEGAWLGSDLVDQGKERLNRL
                     GFFETVDTDTQRVPGSPDQVDVVYKVKERNTGSFNFGIGYGTESGVSFQAGVQQDNWL
                     GTGYAVGINGTKNDYQTYAELSVTNPYFTVDGVSLGGRLFYNDFQADDADLSDYTNKS
                     YGTDVTLGFPINEYNSLRAGLGYVHNSLSNMQPQVAMWRYLYSMGEHPSTSDQDNSFK
                     TDDFTFNYGWTYNKLDRGYFPTDGSRVNLTGKVTIPGSDNEYYKVTLDTATYVPIDDD
                     HKWVVLGRTRWGYGDGLGGKEMPFYENFYAGGSSTVRGFQSNTIGPKAVYFPHQASNY
                     DPDYDYECATQDGAKDLCKSDDAVGGNAMAVASLEFITPTPFISDKYANSVRTSFFWD
                     MGTVWDTNWDSSQYSGYPDYSDPSNIRMSAGIALQWMSPLGPLVFSYAQPFKKYDGDK
                     AEQFQFNIGKTW"
     gene            43009..43494
                     /gene="skp"
                     /locus_tag="ACHOSC_RS00460"
                     /old_locus_tag="pgaptmp_000092"
                     /gene_synonym="hlpA"
                     /gene_synonym="ompH"
     CDS             43009..43494
                     /gene="skp"
                     /locus_tag="ACHOSC_RS00460"
                     /old_locus_tag="pgaptmp_000092"
                     /gene_synonym="hlpA"
                     /gene_synonym="ompH"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone Skp"
                     /protein_id="HGU4546108.1"
                     /translation="MKKWLLAAGLGLALATSAQAADKIAIVNMGSLFQQVAQKTGVSN
                     TLENEFKGRASELQRMETDLQAKMKKLQSMKAGSDRTKLEKDVMAQRQTFAQKAQAFE
                     QDRARRSNEERGKLVTRIQTAVKSVANSQDIDLVVDANAVAYNSSDVKDITADVLKQV
                     K"
     gene            43498..44523
                     /gene="lpxD"
                     /locus_tag="ACHOSC_RS00465"
                     /old_locus_tag="pgaptmp_000093"
     CDS             43498..44523
                     /gene="lpxD"
                     /locus_tag="ACHOSC_RS00465"
                     /old_locus_tag="pgaptmp_000093"
                     /EC_number="2.3.1.191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016410 - N-acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-3-O-(3-hydroxymyristoyl)glucosamine
                     N-acyltransferase"
                     /protein_id="HGU4546109.1"
                     /translation="MPSIRLADLAQQLDAELHGDGDIVITGVASMQSAQTGHITFMVN
                     PKYREHLGLCQASAVVMTQDDLPFAKSAALVVKNPYLTYARMAQILDTTPQPAQNIAP
                     SAVIDATAKLGNNVSIGANAVIESGVELGDNVIIGAGCFVGKNSKIGAGSRLWANVTI
                     YHEIQIGQNCLIQSGTVVGADGFGYANDRGNWVKIPQIGRVIIGDRVEIGACTTIDRG
                     ALDDTIIGNGVIIDNQCQIAHNVVIGDNTAVAGGVIMAGSLKIGRYCMIGGASVINGH
                     MEICDKVTVTGMGMVMRPITEPGVYSSGIPLQPNKVWRKTAALVMNIDDMSKRLKSLE
                     RKVNQQD"
     gene            44628..45083
                     /gene="fabZ"
                     /locus_tag="ACHOSC_RS00470"
                     /old_locus_tag="pgaptmp_000094"
     CDS             44628..45083
                     /gene="fabZ"
                     /locus_tag="ACHOSC_RS00470"
                     /old_locus_tag="pgaptmp_000094"
                     /EC_number="4.2.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000210741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-ACP dehydratase FabZ"
                     /protein_id="HGU4546110.1"
                     /translation="MTTNTHTLQIEEILELLPHRFPFLLVDRVLDFEEGRFLRAVKNV
                     SVNEPFFQGHFPGKPIFPGVLILEAMAQATGILAFKSVGKLEPGELYYFAGIDEARFK
                     RPVVPGDQMIMEVTFEKTRRGLTRFKGVALVDGKVVCEATMMCARSREA"
     gene            45087..45875
                     /gene="lpxA"
                     /locus_tag="ACHOSC_RS00475"
                     /old_locus_tag="pgaptmp_000095"
     CDS             45087..45875
                     /gene="lpxA"
                     /locus_tag="ACHOSC_RS00475"
                     /old_locus_tag="pgaptmp_000095"
                     /EC_number="2.3.1.129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008780 -
                     acyl-[acyl-carrier-protein]-UDP-N-acetylglucosamine
                     O-acyltransferase activity [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-ACP--UDP-N-acetylglucosamine
                     O-acyltransferase"
                     /protein_id="HGU4546111.1"
                     /translation="MIDKSAFVHPTAIVEEGASIGANAHIGPFCIVGPHVEIGEGTVL
                     KSHVVVNGHTKIGRDNEIYQFASIGEVNQDLKYAGEPTRVEIGDRNRIRESVTIHRGT
                     VQGGGLTKVGSDNLLMINAHIAHDCTVGNRCILANNATLAGHVSVDDFAIIGGMTAVH
                     QFCIIGAHVMVGGCSGVAQDVPPYVIAQGNHATPFGVNIEGLKRRGFSREAITAIRNA
                     YKLIYRSGKTLDEVKPEIAELAETYPEVKAFTDFFARSTRGLIR"
     gene            45875..47023
                     /gene="lpxB"
                     /locus_tag="ACHOSC_RS00480"
                     /old_locus_tag="pgaptmp_000096"
     CDS             45875..47023
                     /gene="lpxB"
                     /locus_tag="ACHOSC_RS00480"
                     /old_locus_tag="pgaptmp_000096"
                     /EC_number="2.4.1.182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008915 - lipid-A-disaccharide synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipid-A-disaccharide synthase"
                     /protein_id="HGU4546112.1"
                     /translation="MTEQRPLTIALVAGETSGDILGAGLIRALKERVPNARFVGVAGP
                     RMQAEGCEAWYEMEELAVMGIVEVLGRLRRLLHIRADLTKRFGELKPDIFVGIDAPDF
                     NITLEGNLKKQGIKTIHYVSPSVWAWRQKRVFKIGRATDLVLAFLPFEKAFYDKYNVP
                     CRFIGHTMADAMPLDPDKNAARDVLGIPHDAHCLALLPGSRGAEVEMLSADFLKTAQL
                     LRQTYPDLEIVVPLVNAKRREQFERIKAEVAPDLSVHLLDGMGREAMVASDAALLASG
                     TAALECMLAKCPMVVGYRMKPFTFWLAKRLVKTDYVSLPNLLAGRELVKELLQEECEP
                     QKLAAALLPLLANGKTSHAMHDTFRELHQQIRCNADEQAAQAVLELAQ"
     gene            47020..47616
                     /gene="rnhB"
                     /locus_tag="ACHOSC_RS00485"
                     /old_locus_tag="pgaptmp_000097"
     CDS             47020..47616
                     /gene="rnhB"
                     /locus_tag="ACHOSC_RS00485"
                     /old_locus_tag="pgaptmp_000097"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004523 - RNA-DNA hybrid ribonuclease
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HII"
                     /protein_id="HGU4546113.1"
                     /translation="MIEFVYPHTQLVAGVDEVGRGPLVGAVVTAAVILDPARPIAGLN
                     DSKKLSEKRRLALYEEIKEKALSWSLGRAEPHEIDELNILHATMLAMQRAVAGLHIAP
                     EYVLIDGNRCPKLPMPAMAVVKGDSRVPEISAASILAKVTRDAEMAALDIVFPQYGFA
                     QHKGYPTAFHLEKLAEHGATEHHRRSFGPVKRALGLAS"
     gene            47653..51135
                     /gene="dnaE"
                     /locus_tag="ACHOSC_RS00490"
                     /old_locus_tag="pgaptmp_000098"
     CDS             47653..51135
                     /gene="dnaE"
                     /locus_tag="ACHOSC_RS00490"
                     /old_locus_tag="pgaptmp_000098"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit alpha"
                     /protein_id="HGU4546114.1"
                     /translation="MSEPRFVHLRVHSDYSMIDGLAKTAPLVKKAAALGMPALAITDF
                     TNLCGLVKFYGAGHGAGIKPIVGADFNVQCDLLGDELTHLTVLAANNTGYQNLTLLIS
                     KAYQRGYGAAGPIIDRDWLIELNEGLILLSGGRMGDVGRSLLRGNRALVDECVAFYEE
                     HFPDRYFLELIRTGRPDEESYLHAAVELAEARGLPVVATNDVRFIDSSDFDAHEIRVA
                     IHDGFTLDDPKRPRNYSPQQYMRSEEEMCELFADIPEALANTVEIAKRCNVTVRLGEY
                     FLPQFPTGDMSTEDYLVKRAKEGLEERLAFLFPDEEERVKRRPEYDERLETELQVINQ
                     MGFPGYFLIVMEFIQWSKDNGVPVGPGRGSGAGSLVAYALKITDLDPLEFDLLFERFL
                     NPERVSMPDFDVDFCMEKRDQVIEHVADMYGRDAVSQIITFGTMAAKAVIRDVGRVLG
                     HPYGFVDRISKLIPPDPGMTLAKAFEAEPQLPEIYEADEEVKALIDMARKLEGVTRNA
                     GKHAGGVVIAPTKITDFAPLYCDEEGKHPVTQFDKSDVEYAGLVKFDFLGLRTLTIIN
                     WALEMINKRRAKNGEPPLDIAAIPLDDKKSFDMLQRSETTAVFQLESRGMKDLIKRLQ
                     PDCFEDMIALVALFRPGPLQSGMVDNFIDRKHGREEISYPDVQWQHESLKPVLEPTYG
                     IILYQEQVMQIAQVLSGYTLGGADMLRRAMGKKKPEEMAKQRSVFAEGAEKNGINAEL
                     AMKIFDLVEKFAGYGFNKSHSAAYALVSYQTLWLKAHYPAEFMAAVMTADMDNTEKVV
                     GLVDECWRMGLKILPPDINSGLYHFHVNDDGEIVYGIGAIKGVGEGPIEAIIEARNKG
                     GYFRELFDLCARTDTKKLNRRVLEKLIMSGAFDRLGPHRAALMNSLGDALKAADQHAK
                     AEAIGQADMFGVLAEEPEQIEQSYASCQPWPEQVVLDGERETLGLYLTGHPINQYLKE
                     IERYVGGVRLKDMHPTERGKVITAAGLVVAARVMVTKRGNRIGICTLDDRSGRLEVML
                     FTDALDKYQQLLEKDRILIVSGQVSFDDFSGGLKMTAREVMDIDEAREKYARGLAISL
                     TDRQIDDQLLNRLRQSLEPHRSGTIPVHLYYQRADARARLRFGATWRVSPSDRLLNDL
                     RGLIGSEQVELEFD"
     gene            51148..52107
                     /gene="accA"
                     /locus_tag="ACHOSC_RS00495"
                     /old_locus_tag="pgaptmp_000099"
     CDS             51148..52107
                     /gene="accA"
                     /locus_tag="ACHOSC_RS00495"
                     /old_locus_tag="pgaptmp_000099"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase carboxyl transferase
                     subunit alpha"
                     /protein_id="HGU4546115.1"
                     /translation="MSLNFLDFEQPIAELEAKIDSLTAVSRQDEKLDINIDEEVHRLR
                     EKSVELTRKIFADLGAWQIAQLARHPQRPYTLDYVRLAFDEFDELAGDRAYADDKAIV
                     GGIARLDGRPVMIIGHQKGRETKEKIRRNFGMPAPEGYRKALRLMQMAERFKMPIITF
                     IDTPGAYPGVGAEERGQSEAIARNLREMSRLGVPVVCTVIGEGGSGGALAIGVGDKVN
                     MLQYSTYSVISPEGCASILWKSADKAPLAAEAMGIIAPRLKELKLIDSIIPEPLGGAH
                     RNPEAMAASLKAQLLADLADLDVLSTEDLKNRRYQRLMSYGYA"
     gene            52206..54347
                     /gene="ldcC"
                     /locus_tag="ACHOSC_RS00500"
                     /old_locus_tag="pgaptmp_000100"
     CDS             52206..54347
                     /gene="ldcC"
                     /locus_tag="ACHOSC_RS00500"
                     /old_locus_tag="pgaptmp_000100"
                     /EC_number="4.1.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine decarboxylase LdcC"
                     /protein_id="HGU4546116.1"
                     /translation="MNIIAIMGPHGVFYKDEPIKELESALVAQGFQIIWPQNSVDLLK
                     FIEHNPRICGVIFDWDEYSLDLCSDINQLNEYLPLYAFINTHSTMDVSVQDMRMALWF
                     FEYALGQAEDIAIRMRQYTDEYLDNITPPFTKALFTYVKERKYTFCTPGHMGGTAYQK
                     SPVGCLFYDFFGGNTLKADVSISVTELGSLLDHTGPHLEAEEYIARTFGAEQSYIVTN
                     GTSTSNKIVGMYAAPSGSTLLIDRNCHKSLAHLLMMNDVVPVWLKPTRNALGILGGIP
                     RREFTRDSIEEKVAATTQAQWPVHAVITNSTYDGLLYNTDWIKQTLDVPSIHFDSAWV
                     PYTHFHPIYQGKSGMSGERVAGKVIFETQSTHKMLAALSQASLIHIKGEYDEEAFNEA
                     FMMHTTTSPSYPIVASVETAAAMLRGNPGKRLINRSVERALHFRKEVQRLREESDGWF
                     FDIWQPPQVDEAECWPVALGEQWHGFNDADADHMFLDPVKVTILTPGMDEQGNMSEEG
                     IPAALVAKFLDERGIVVEKTGPYNLLFLFSIGIDKTKAMGLLRGLTEFKRSYDLNLRI
                     KNMLPDLYAEDPDFYRNMRIQDLAQGIHKLIRKHDLPGLMLRAFDTLPEMIMTPHQAW
                     QRQIKGEVETIALEQLVGRVSANMILPYPPGVPLLMPGEMLTKESRTVLDFLLMLCSV
                     GQHYPGFETDIHGAKQDEDGVYRVRVLKMAG"
     gene            54404..54793
                     /gene="yaeR"
                     /locus_tag="ACHOSC_RS00505"
                     /old_locus_tag="pgaptmp_000101"
     CDS             54404..54793
                     /gene="yaeR"
                     /locus_tag="ACHOSC_RS00505"
                     /old_locus_tag="pgaptmp_000101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308216.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HGU4546117.1"
                     /translation="MLGLKQVHHIAIIATDYAVSKAFYCDILGFTLQSEVYREARDSW
                     KGDLALNGQYVIELFSFPFPPERPSRPEACGLRHLAFSVDDIDAAVAHLESHNVKCEA
                     IRVDPYTQKRFTFFNDPDGLPLELYEQ"
     gene            54858..56156
                     /gene="tilS"
                     /locus_tag="ACHOSC_RS00510"
                     /old_locus_tag="pgaptmp_000102"
     CDS             54858..56156
                     /gene="tilS"
                     /locus_tag="ACHOSC_RS00510"
                     /old_locus_tag="pgaptmp_000102"
                     /EC_number="6.3.4.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016879 - ligase activity, forming
                     carbon-nitrogen bonds [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA lysidine(34) synthetase TilS"
                     /protein_id="HGU4546118.1"
                     /translation="MTLTLNRQLLTSRQILVAFSGGLDSTVLLHQLVQWRTENPGVTL
                     RAIHVHHGLSANADAWVTHCENVCQQWQVPLVVERVQLAQEGLGIEAQARQARYQAFA
                     RTLLPGEVLVTAQHLDDQCETFLLALKRGSGPAGLSAMAEVSEFAGTRLIRPLLARTR
                     GELEQWALAHGLRWIEDESNQDDSYDRNFLRLRVVPLLQQRWPHFAEATARSAALCAE
                     QESLLDELLADDLAHCQTSQGTLQIAPMLAMSDARRAAIIRRWLAGQNAPMPSRDALV
                     RIWQEVALAREDASPCLRLGAFEIRRYQSQLWWIKSVTGQSETIVPWQTWLQPLELPA
                     GLGSVQLTAGGDIRPPRADEAVSVRFKAAGLLHIVGRNGGRKLKKIWQELGVPPWLRD
                     TTPLLFYGETLIAAAGVFVTQEGVAEGENGVSFVWQKTLS"
     gene            complement(56205..56465)
                     /gene="rof"
                     /locus_tag="ACHOSC_RS00515"
                     /old_locus_tag="pgaptmp_000103"
     CDS             complement(56205..56465)
                     /gene="rof"
                     /locus_tag="ACHOSC_RS00515"
                     /old_locus_tag="pgaptmp_000103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rho-binding antiterminator"
                     /protein_id="HGU4546119.1"
                     /translation="MSMNDTYQPINCDDYDNLELACQHHLMLTLELKDGEKLQAKASD
                     LVSRKNVEYLVVEAAGETRELRLDKITSFSHPEIGTVVVSES"
     gene            complement(56452..56652)
                     /gene="yaeP"
                     /locus_tag="ACHOSC_RS00520"
                     /old_locus_tag="pgaptmp_000104"
     CDS             complement(56452..56652)
                     /gene="yaeP"
                     /locus_tag="ACHOSC_RS00520"
                     /old_locus_tag="pgaptmp_000104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012133967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YaeP family protein"
                     /protein_id="HGU4546120.1"
                     /translation="MEKYCELIRKRYAEIASGDLGYVPDALGCVLKVLNEMAADDALS
                     EAVREKAAYAAANLLVSDYVNE"
     gene            56818..57363
                     /gene="yaeQ"
                     /locus_tag="ACHOSC_RS00525"
                     /old_locus_tag="pgaptmp_000105"
     CDS             56818..57363
                     /gene="yaeQ"
                     /locus_tag="ACHOSC_RS00525"
                     /old_locus_tag="pgaptmp_000105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YaeQ family protein"
                     /protein_id="HGU4546121.1"
                     /translation="MALKATIYKATVNVADLDRNQFLDASLTLARHPSETQERMMLRL
                     LAWLKYADERLQFTRGLCADDEPEAWLRNDHLGIDLWIELGLPDERRIKKACTQAAEV
                     ALFTYNSRAAQIWWQQNQSKCVQFANLSVWYLDDEQLAKVSAFADRTMTLQATIQDGV
                     IWLSDDKNNLEVNLTAWQQPS"
     gene            57360..57782
                     /gene="arfB"
                     /locus_tag="ACHOSC_RS00530"
                     /old_locus_tag="pgaptmp_000106"
     CDS             57360..57782
                     /gene="arfB"
                     /locus_tag="ACHOSC_RS00530"
                     /old_locus_tag="pgaptmp_000106"
                     /EC_number="3.1.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003747 - translation release factor
                     activity [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alternative ribosome rescue aminoacyl-tRNA
                     hydrolase ArfB"
                     /protein_id="HGU4546122.1"
                     /translation="MIVISRHVAIPDGELEITAIRAQGAGGQHVNKTSTAIHLRFDIR
                     ASSLPEYYKERLLAASHHLISSDGVIVIKAQEYRSQELNREAALARLVAVIKDLTTEQ
                     KARRPTRPTRASKERRLASKAQKSSVKAMRGKVRSGRE"
     gene            57796..58506
                     /gene="nlpE"
                     /locus_tag="ACHOSC_RS00535"
                     /old_locus_tag="pgaptmp_000107"
                     /gene_synonym="cutF"
     CDS             57796..58506
                     /gene="nlpE"
                     /locus_tag="ACHOSC_RS00535"
                     /old_locus_tag="pgaptmp_000107"
                     /gene_synonym="cutF"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706138.1"
                     /note="NlpE, an outer membrane lipoprotein, interacts
                     directly with CpxA, the sensor histidine kinase of the Cpx
                     system for response to envelope stress; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="envelope stress response activation lipoprotein
                     NlpE"
                     /protein_id="HGU4546123.1"
                     /translation="MVKKAIVTAMAVISLFTLMGCNNRAEVDTLSPAQAAELKPMPQS
                     WRGVLPCADCEGIETSLFLEKDGTWVMNERYLGAREEPSSFASYGTWARTADKLVLTD
                     SKGEKSYYRAKGDALEMLDREGNPIESQFNYTLEPAQSSLPMTPMTLRGMYFYMADAA
                     TFTDCATGKRFMVANNAELERSYLAARGHSEKPVLLSVEGHFTLEANPDTGAPTKVLA
                     PDTAGKFYPNKDCSSLGQ"
BASE COUNT        13739 a        14539 c        16305 g        13950 t
ORIGIN      
        1 gtatgtaaga cttcacgcaa cgtctcccca tcaggaaacg ccagctccgg tgcaatctca
       61 aacagcggcc acagcataaa tccacgattc ttcatatcgt agtgcggaac ggccaggcgt
      121 tcggtattta tcacttcatt accaaacagc atgatatcga gatccagcgt gcgtggtccc
      181 cagcgttcag ctttacgaac acgaccctgt tgcagttcta tgcgctgtgt atggttgagt
      241 aactcttccg gggcgagcga agtttcaagc gccacggcgg cgttgaggta atcgggttga
      301 tcttgcggcc ccagcggtgg ggtgcggtaa aacgaagaaa cggcaagaat gtggctttca
      361 gggatatcgc ctaatgcttt cagggcagca ttgacctgct ccagcggaga ggccagattg
      421 ctgcctatgg caatatacgc cactgtcatg cggtaccctc acgacgcggt gcgcgtttgc
      481 gtggacgacg agtacggcga cgcggtgacg gctcttcatc caactcgtta agcatccctt
      541 tttgatctgg tggcgcggaa acctggaact caccccacca tttcaccaga cgctgcagtt
      601 cagcgttacg ttcaacttca gctcgcaagg ccaacaggtc ataagccgca cggaacttag
      661 gatgctccag cagtttccat gcgcgtttac cctgacgacg ggacatacgc aactgcaact
      721 gccagatatc gcgggttaac gtcgtcagac gtttcgggat tgccagtgaa cggcaggctt
      781 cgtccagcac gtcgttcatc gccagcgcga aagcgtcgtg ataggtcagg ccgctttcct
      841 gggcaatctt ctgtgccgtc tccagcagtg ggtaccagaa catggcggca aacaggaacg
      901 ccgggttcac gcgcatatcg ttatggatac gcgtatcggt attcttcagc acctgttcaa
      961 tgatccgctc catcgggctg tcgccatttt ccgtgaagta gcgggtaatg gtcgggaaca
     1021 gcggctggaa cagatgatat tcacacaaca gcttataggt atcgtaaccg tagcccgctt
     1081 gtagcagttt aagcgattct tcaaacaggc gtgccggtgg gatatcgttc aacagggtag
     1141 cgaggcgagg gatcggttct gcggtttccg ggctgatgcg catacccaat ttggcggcaa
     1201 aacgtaccgc gcgcagcata cgtaccggat cttcacggta gcgcgtttcc gggttaccaa
     1261 tcagacggat aacgccgtcc ttcagatcct tcataccgcc aacgtaatca cggacggtaa
     1321 aatccgctac gctgtaatag aggctgttga tagtgaaatc gcggcgctgg gcgtcttctt
     1381 cgatggagcc gaaaatgttg tcgcgcagca acatgccgtt ttgcccgcgt tgggaggtcg
     1441 tgcggtcgct gacgttacct tcgtggtgtc cacggaaggt cgcaacttcg ataatctccg
     1501 ggccaaacat tacatgagcc agacggaaac ggcgacccac caggcggcag ttacggaaca
     1561 gtttgcgcac ctgctcaggc gtggcgttag tggttacgtc aaaatctttc ggctttttgc
     1621 caagtaacag gtcgcgcacg ccgccgccaa ccagccaggc ttcgtatccc gctttgttga
     1681 gcctgtacat taccttcagg gcattttcac tgatatcttt gcgggaaata gcatgctgct
     1741 cacgcgggat caccgtcacc tgtggacggg cgactgcctg ttcagcctcg ctttcctcgc
     1801 ggcttagcac cttgcggcaa aaattagcga ctcgggtaaa aatagtacac ctcggtagtg
     1861 tcaaacatca ttcaggacaa aaaaatagcg gctaatcata gctcagcatg acgcatttga
     1921 gaatgttgaa tttacaattg ccgactcggg cacggcggta agccgccagt ttttgacggc
     1981 tgactgaagg atttgctcga cgctgaaatc ctgccagtgt gcttctgcct gctgccccag
     2041 aaattgaagt gccgcgatta gtaccgggcg tggatcgcct ttcggcaacg caggcgcatg
     2101 attctgcttg gaaagtttag cgccttgtgg attaagcgcc agcggcagat gaatgtaatc
     2161 tggcactttc cagccaaaaa gctggtacag cgagatttgc cttactgttg gttcaatcag
     2221 atcagcccca cgcactattt ctgtaacgcc ctggaaatga tcatcaacca caacagccag
     2281 gttgtaggcg aacaacccat cacggcgatg aatgataaaa tcttcccgtg ccagtttttc
     2341 gtcggcgtga ataatgccgc gcagctggtc agtaaattgc gtgaccggat gctgctggcg
     2401 gatacgcact gcggcattgt ctggtccatg atgcaacacc cggcaatgac cgtcgtaaat
     2461 accgccaatg ctttgaatac gcgcacgcgt acaggtgcag taataactta gtccttgttc
     2521 atgtaaccag gcgagtgctt cacgataggc gtggtgacgt tgcgattgcc agagaacatc
     2581 gccgtcccag tgcagaccgt aatgttccag ctggcgcagg atagtttctg cggcaccggg
     2641 aacttcacga ggcgggtcga tatcttctat gcgtaccagc cagcgacctt gccgggcgcg
     2701 agcctgcaaa tagctgccga gcgcggcgat cagagagcca aaatgaagct cgccggaagg
     2761 agagggggcg aagcggccaa tatactgtgt gtctgtcatc tctttgaaca aaaaataagg
     2821 cgggagcatt tcccgcctgt ggtaaacgtg atggaacggc tgtaattagc cagccatctg
     2881 tttttcgcga atttcagcca gcgttttgca gtcgatgcac agatcggctg tcgggcgcgc
     2941 ttccagacgg cgaataccaa tttcaacacc gcaggattcg cagtagccga aatcttcgtc
     3001 ttccactttt ttcagcgtct tctcgatctt tttgatcagc ttacgctcgc gatcgcggtt
     3061 acgcagttcg aggctgaact cttcttcctg ggctgcacgg tctaccgggt ccgggaagtt
     3121 ggctgcttca tcctgcatat gtgtaacggt gcgatcgact tcatccctga gttgattacg
     3181 ccatgcttcc agaatacgac ggaagtgcgc cagctgggct tcattcatat actcttcgcc
     3241 cggcttctcc tgatatggtt ccaccccagc gatggcgaga atactcaggg acgatgtttt
     3301 acggttttgc ccttcttgca tgttgcttct ccttaacacg cactatcgat ccccatgttc
     3361 gggggaaaaa tgaggccgct ataaatagca gatgcttttc cggatagcaa ttatctaaac
     3421 gtaacacttg acaactgtgt gaggaaaagc gtatttgcgc acgcgaccag aatgtaaatt
     3481 aaccagttac ttactttact acaatgtaac cggcagtgat tttttaagag ccatgccttc
     3541 agcagaaatt tccgctttgt aagccagaat ttctaccccc ctctgttgag cttctgacaa
     3601 tagttgcgcg tatttctcat cgatgtggcg cgcgggtgaa aaccgtgtaa tggctgaatg
     3661 cagcacggcg aaaaagataa ccgcacgctg gccttcagcc gctacgctca tcaactcccg
     3721 aaggtgtttc tgacctcgtt cagtgaccgc atcgggaaaa tatccctgtt cgttctccgc
     3781 taacgtaacc gatttcactt caatatagca gtctggacgc gaatccgcct gcaacataaa
     3841 gtcaatacgg ctgcgttctg cgccgtattt tacttcgctt ttcagcgagc tatagcctga
     3901 cagttctgaa attgattcat taaggatagc ctctttcgtc aacctgttag cccaaagcgt
     3961 gttgacgcaa ataaatgcgc cgctctggct ttgagttaat tcccaggtgt gtgggtattt
     4021 ccgtttggtg ttgtctgaag tcgaatacca gacggtatcg ccaggcgttg cacaaccggt
     4081 cattgcaccc gtattcgggc agtgtagcgt taattcgcga ccatcgggtg tgatcacatc
     4141 ggctaaaaaa cgtttgtaac gctgaattag cgtcgcgcgc tgtagagggg gagaaaattc
     4201 cattgcgaca atccttgtta ttgcgttagc gcccagcgtt ttagcggcgt gtagcgtgtg
     4261 cgtccacggg caaacgagga ggcgtaaagg gtgaactccg tcaccgcata cgaccagtta
     4321 aaacctggcg gcgggattgt caccgcctcg ctggcgtcgc gcaataaggt aatatgtgga
     4381 tgaaacggac gattgctttg aaaacaaccg ctgcgggcag cctgtgaacg gagcatattc
     4441 gccagctgga ttaagccgcg tggcggctga cgcatcccta accacaccac acgcgaacgc
     4501 agccattgtc cggcgtcatc aagcgtgagt gtgaaaccag gttgacgaat ccgtccggct
     4561 aaaagagaaa gcgccttctc tttctcggcg ctcacttcgc ctaaaaatgc cagagtcaga
     4621 tgcaaattat cggcggcgac cggacgtccc gcctcaggtg ggaagtgtgt ggcgcgccag
     4681 tggataatct gttcgcggat ttctgcaggt aagtcgatag caaagaacag acgttgcggt
     4741 tcagacatgt gaggcactcg gttatgaatt accgcgatgc tacaatgtgg cgcgaagaat
     4801 gttaaccctc tggagcgttt tgtgtcgtcg ttgcccgttg ctgccgtctt acctgaatta
     4861 cttaccgctc tcgattgtgc gccgcaggta ttattaagtg cgccgaccgg ggccgggaaa
     4921 tcaacctggc tgccgctgca actgctggcg catcccggca ttaacgggaa aattatcctg
     4981 ctggagccgc gtcgtctggc ggcgcgtaac gtcgcgcaac ggctggcgga gctgcttaac
     5041 gaaaagccag gcgataccgt tggctaccgg atgcgtgcgc aaaactgcgt cgggccgaat
     5101 acccgcctgg aagtagttac cgaaggcgtg ctgacgcgga tgatccagcg tgacccggag
     5161 ttgagcggtg ttggattggt gatcctcgat gagtttcatg agcgcagctt gcaggcggat
     5221 ttggcgttgg cgctgttact cgatgtgcaa caaggtctgc gtgatgacct taaactgctg
     5281 attatgtcgg ctacgctgga caacgaccgc ttgcagcaaa tgctgccaga agcgcccgtc
     5341 gtcatctcag aagggcgctc gtttccggtt gaacgccgtt atttaccact gcccacgcat
     5401 cagcgttttg acgaagccgt tgcggtagcc accgctgaaa tgctgcgtca ggaaagtgga
     5461 tcattactgt tatttttacc tggcgtcgga gaaattcagc gtgtgcagga acaactggct
     5521 tcgcgcatcg gcagcgatgt attgctctgc ccgctgtatg gcgcgttgtc gctgaacgat
     5581 cagcgaaaag cgatcctccc ggcaccgcaa gggatgcgca aagtggtgct ggcgaccaat
     5641 attgctgaaa ccagtttaac catcgaaggt attcgtctgg tggtggattg tgcccaggag
     5701 cgtgtggcgc gttttgatcc gcgcacgggg cttacgcgac tgattactca acgcgttagc
     5761 caggcatcca tgacgcagcg tgccgggcgc gccgggcgtc tggagccggg tatctgcctg
     5821 catttaatcg ccaaggaaca agcagaacgc gccgcggcgc aaagtgaacc ggagatatta
     5881 caaagcgatc tttccggttt gctgatggaa ttactgcaat ggggatgcag cgatccggcg
     5941 cagatgagct ggctggatca accgccagta gtgaatctac tggccgcgaa acgtctgtta
     6001 caaatgctgg gggcactgga gggtgaacgg cttagtgcgc aagggcaaaa aatggcagcg
     6061 ctgggtaacg atccgcgttt agcggcaatg ctggttagcg cgaagaacga cgacgaagct
     6121 gctaccgcgg caaaaattgc cgccattctc gaagagccgc cacggatggg caatagtgac
     6181 ctgggcgtgg cgttttcgcg caatcaacca gcctggcagc aacgtagtca gcaactgtta
     6241 aaacgcttaa acgtacgtgg cggtgaggca gacagttcgc ttatcgcgcc gctacttgcc
     6301 ggggcgtttg ccgatcgcat tgctcgtcgc cgtgggcaag atggacgcta tcaactggca
     6361 aacggcatgg gagcgatgct cgatgccaac gacgcgctaa gccgccacga atggttgatc
     6421 gcaccgttat tattgcaggg cagcgcctcg ccggatgcgc ggattttact ggcgctgccg
     6481 gtcgatattg atgagttagt acaacgctgc ccgcagctgg tacagcagtc tgacactgtg
     6541 gagtgggatg acgcgcaagg tacgctgaaa gcctggcgtc ggctgcaaat cggtctgctg
     6601 acggtgaaag tgcagccgct ggcgaaaccc tcggaagacg agttgcatca ggcgatgctt
     6661 aatggcatcc gcgataaaga tttaagcgtg ctcaactgga cggcggaagc ggaacagcta
     6721 cgcttgcgtt tgttatgcgc cgcaaagtgg ttgccggaat atgactggcc agcggttgat
     6781 gatgaaagtt tattggcgac gctggaaacg tggctgctgc cacatatgac tggcgtacat
     6841 tcactacgcg gcctgaaatc actcgacatt tatcaggcac tacgcggatt acttgattgg
     6901 ggaatgcagc aacgtctgga tagtgaattg cctgcgcatt acactgtgcc gacgggaagc
     6961 cggatcgcca ttcgttatca tgaagataac ccgcccgcgc tggcggtgag aatgcaagag
     7021 atgtttggcg aggccaccaa tccgacgatc gcccaggggc gcgtgccgct ggtgctggag
     7081 ttgctttcac ctgcccaaag gccattacaa atcacacgag atttgagcgc cttctggaaa
     7141 ggagcgtacc gtgaggtgca aaaagagatg aaagggcgtt atcccaaaca tgtctggccg
     7201 gacgacccgg caaatactgc accgacgcga cggacgaaaa agtattcgta aggttggttt
     7261 tctccctctc cctgtgggag aggggaccga tcgagcacaa attttgagag atatcttctt
     7321 ctgtcttgta acagaagaac agaaaatcgg gcttttgcgc ctgaatattg cggagaaaaa
     7381 gcatggccgg gaatgaccgc gagccaattg gacgcaaagg gaaaccgacg cgtccggtca
     7441 aacaaaaggt aagccgtcgt cgttacgaag atgacgatga ttacgacgat tatgatgact
     7501 atgaggatga agaaccgatg ccgcgcaaag gtaagggcaa aggcaaaggg cgtaagcctc
     7561 gtggcaaacg cggctggcta tggctgctgt taaaactggc tatcgttttt gccgtgctga
     7621 tcgccattta cggcgtttat ctcgatcaaa aaattcgtag ccgtattgat ggcaaggtct
     7681 ggcaactgcc tgcggcagtt tatggccgaa tggtcaacct tgagccagat atgaccatca
     7741 gcaagaacga gatggtgaag ctgctggagg cgactcagta tcgtcaggtg tcgaaaatga
     7801 cccgtcctgg cgaatttacc gtgcaggcca acagcattga gatgatccgc cgtccgtttg
     7861 atttcccgga cagtaaagaa gggcaggtgc gtgcgcgtct gacctttgat ggcgatcatc
     7921 tggcgacgat cgtcaatatg gagaacaacc gtcagttcgg tttcttccgt cttgatccgc
     7981 gtctgatcac catgatctcc tcgccaaacg gtgagcagcg tttgtttgtg ccgcgcagtg
     8041 gtttccctga tttgctggtg gatactttgc tggcgacaga agaccgtcat ttttacgagc
     8101 atgacggcat cagcctctac tccatcggac gtgcggtgct ggcaaacctg accgccggac
     8161 gcacggtgca gggagcgagt acgctgacgc aacagctggt gaaaaacctg ttcctctcca
     8221 gtgagcgttc ttactggcgt aaagcgaacg aagcttacat ggcgctgatc atggacgcgc
     8281 gttacagcaa agaccgtatt cttgagctgt atatgaacga ggtgtatctc ggtcagagcg
     8341 gcgacaacga aatccgcggc ttcccgctgg cgagcttgta ttactttggt cgcccggtag
     8401 aagagctaag cctcgaccag caggcgctgt tagtcggcat ggtgaaagga gcgtccatct
     8461 acaacccgtg gcgtaaccca aaactggcgc tggagcgacg taatctggtg ctgcgtctgc
     8521 tgcaacagca acagattatt gatcaagaac tctatgacat gttgagtgca cgtccgctgg
     8581 gtgttcagcc acgcggtggg gtgatctctc cacagccagc ctttatgcaa ctggtgcgtc
     8641 aggagctgca ggcgaaatta ggcgataagg taaaagatct ctccggcgtg aagatcttca
     8701 ctacctttga ctcggtggcc caggacgcgg cagaaaaagc cgccgtggaa ggcattccgg
     8761 cactgaagaa acagcgtaag ttgagcgatc ttgaaactgc gattgtggtc gtcgaccgct
     8821 ttagtggtga agttcgtgcg atggtcggtg gttctgagcc gcagtttgcg ggctacaacc
     8881 gtgcgatgca ggcgcgtcgt tcgattggtt cccttgcaaa accagcgact tatctgactg
     8941 ccttaagcca gccgaaaatc tatcgtctga atacgtggat tgcggatgcg ccaatcgcgc
     9001 tgcgtcagcc gaatggccag gtctggtcac cgcagaatga tgaccgtcgt tatagcgaaa
     9061 gcggcagagt gatgctggtg gatgcgttga cccgttcgat gaacgtgccg acggtaaatc
     9121 tggggatggc gctggggctg cctgcggtta cggagacctg gatcaaactg ggcgtaccga
     9181 aagatcagtt gcatccggtt ccggcgatgc tgctgggggc gttgaactta acgccaatcg
     9241 aagtggcgca ggcgttccag accatcgcca gcggcggtaa ccgtgcaccg ctttctgcac
     9301 tgcgttcggt aattgcggaa gatggcaagg tgctgtatca gagcttcccg caggcggaac
     9361 gcgctgttcc ggcgcaggcg gcgtatctga cactatggac catgcagcag gtggtacaac
     9421 gcggtacggg tcgtcagctt ggggcgaaat acccgaacct gcatctggca gggaaaacag
     9481 ggactaccaa caataacgta gatacctggt ttgcaggcat tgacggcagc acggtgacca
     9541 tcacctgggt aggccgtgat aacaaccagc cgaccaaact gtatggagcc agcggggcaa
     9601 tgtcgattta tcagcgttat ctggctaacc agacgccaac gccgctgaat cttgttccgc
     9661 cagaagatat tgcagatatg ggcgtggact acgacggcaa ctttgtttgc agcggtggca
     9721 tgcgtgtctt gccggtctgg accagcgatc cgcaatcgct gtgccagcag agcgagatgc
     9781 agcagcagcc gtcaggcaat ccgtttgatc agtcttctca gccgcagcaa cagccgcaac
     9841 agcaacctgc tcagcaagag cagaaagaca gcgacggtgt agccggttgg atcaaggata
     9901 tgtttggtag taattaacat ctaagcgtga aataccggat ggcgagttgc catccggtaa
     9961 aataacatcc catctaagat attaaccctt tcttttcatc tggttgttta ttaacccttc
    10021 aggaacgctc agattgcgta ccgcttgcga acccgccagc gtttcgaata ttatcttatc
    10081 tttataataa tcattctcgt ttacgttatc attcacttta catcagagat ataccaatgg
    10141 cgcgttccaa aactgctcag ccaaaacact cactgcgtaa aatcgcagtt gtagtagcca
    10201 cagcggtaag cggcatgtct gtttatgcac aggcagcggt tgaaccgaaa gaagacacta
    10261 tcaccgttac cgttgcacct gcgccgcaag aaagcgcatg ggggcctgct gcaactattg
    10321 cggcgcgaca gtctgctacc ggcactaaaa ccgatacgcc gattcaaaaa gtgccacagt
    10381 ctatttctgt tgtgaccgcc gaagagatgg cgctgcatca gccgaagtcg gtaaaagaag
    10441 cgcttagcta cacgccgggt gtctctgttg gtacgcgtgg cgcatccaac acctatgacc
    10501 acctgatcat tcgcggcttt gcggcagaag gccaaagcca gaataactat ctgaatggcc
    10561 tgaagttgca gggcaacttc tataacgatg cggtcattga cccgtatatg ctggaacgcg
    10621 ctgaaattat gcgtggcccg gtttccgtgc tttacggtaa aagcagtcct ggcggcctgt
    10681 tgaatatggt cagcaagcgt ccgaccaccg aaccgctgaa agaagttcag tttaaagccg
    10741 gtactgacag cctgttccag actggttttg actttagcga ttcgttggat gatgacggtg
    10801 tttactctta tcgcctgacc ggtcttgcgc gttctgccaa tgcccagcag aaagggtcag
    10861 aagagcagcg ttatgctatt gcaccggcgt tcacctggcg tccggatgat aaaaccaatt
    10921 tcaccttcct ttcttacttc cagaacgagc cggaaaccgg ttattacggc tggttgccga
    10981 aagagggaac cgttgagccg ctgccgaacg gtaagcgtct gccgacagac tttaatgaag
    11041 gggcgaagaa caacacctat tctcgtaatg agaagatggt cggctacagc ttcgatcacg
    11101 aatttaacga cacctttact gtgcgtcaga acctgcgctt tgctgaaaac aaaacctcgc
    11161 aaaacagcgt ttatggttac ggcgtctgct ccgatccggc gaatgcttac agcaaacagt
    11221 gtgcggcatt agcgccagcg gataaaggcc attatctggc acgtaaatac gtcgttgatg
    11281 atgagaagct gcaaaacttc tccgttgata cccagttgca gagcaagttt gccactggcg
    11341 atatcgacca caccctgctg tccggtgtcg actttatgcg tatgcgtaat gacatcaacg
    11401 cctggtttgg ttacgacgac tctgtgccac tgctcaatct gtacaatccg gtgaataccg
    11461 atttcgactt caatgccaaa gatccggcaa actccggccc ttaccgcatt ctgaataaac
    11521 agaaacaaac gggcgtttat gttcaggatc aggcgcagtg ggataaagtg ctggtcaccc
    11581 taggcggtcg ttatgactgg gcagatcaag aatctcttaa ccgcgttgct gggacgaccg
    11641 ataaacgtga tgacaaacag tttacctggc gtggtggtgt taactacctg tttgataatg
    11701 gtgtaacacc ttacttcagc tatagcgaat cgtttgaacc ttcttcgcaa gttgggaagg
    11761 atggtaatat tttcgcaccg tctaaaggta agcagtatga agtcggcgtg aaatatgtac
    11821 cggaagatcg tccgattgta gttactggtg ccgtgtataa tctcactaaa accaacaacc
    11881 tgatggcgga ccctgagggt tccttcttct cggttgaagg tggcgagatc cgcgcacgtg
    11941 gcgtagaaat cgaagcgaaa gcggcgctgt cggcgagtgt taacgtagtc ggttcttata
    12001 cttacaccga tgcggaatac accaccgata ctacctataa aggcaatacg cctgcacagg
    12061 tgccaaaaca catggcttcg ctgtgggctg actatacctt ctttgacggt ccgctttcag
    12121 gtctgacgct gggcaccggt ggtcgttata ctggctccag ctatggtgat ccggctaact
    12181 cctttaaagt gggaagttat acggtcgtgg atgcgttagt gcgttatgat ctggcgcgag
    12241 tcggcatggc gggctccaac gtggcgctgc atgttaacaa cctgttcgat cgtgaatacg
    12301 tcgccagctg ctttaacact tatggctgct tctggggcgc agaacgtcag gtcgttgcaa
    12361 ccgcaacctt ccgtttctaa tttctctttt ggggcacgga tttccgtgcc catttcacaa
    12421 gttggctgtt atgcaggaat acacgaatca ttccgatacc acttttgcac tgcgtaatat
    12481 ctcctttcgt gtgcccgggc gcacgctttt gcatccgctg tcgttaacct ttcctgccgg
    12541 gaaagtgacc ggtctgattg gtcacaacgg ttctggtaaa tccactctgc tcaaaatgct
    12601 tggccgtcat cagcctccgt cagaagggga gattcttctt gatgcccagc cgctggaaag
    12661 ctggagcagc aaagcgtttg cccgcaaagt ggcttatttg ccgcagcagc ttcctccggc
    12721 agaagggatg accgtgcgtg aactggtggc gattggtcgt tacccgtggc atggcgcgct
    12781 ggggcgcttt ggtgcggcag atcgcgaaaa ggtcgaggaa gctatctcgc tggttggctt
    12841 aaaaccgctg gcgcatcggc tggtcgatag tctctctggc ggcgaacgtc agcgggcgtg
    12901 gatcgccatg ctggtggcgc aggatagccg ttgtctgttg ctcgacgaac cgacttcggc
    12961 gctggatatc gcccaccagg ttgatgtgct ggcgctggtg caccgtttaa gtcaggaacg
    13021 tggcctgacg gtcattgccg tgctgcacga tatcaatatg gcggcacgct actgtgatta
    13081 tctggtcgcc ctgcgcggtg gtgaaatgat tgctcaggga acgcctgcgg aaattatgcg
    13141 cggcgaaacc ctcgaaatga tttatggcat cccgatgggt attttgccgc atccggcggg
    13201 tgctgcacct gtgagttttg tttattgatg agcggcttac ctcttatttc gcgccgtcga
    13261 ctgttaacgg cgatggcgct ttctccgttg ttatggcaga tgaataccgc ccacgcggcg
    13321 gctattgacc ccaatcgtat tgtggcgctg gagtggttgc cggtggaatt actgctggcg
    13381 ctcggcatcg tgccttacgg cgtggcggat accatcaact atcgcctgtg ggtcagcgaa
    13441 ccaccattgc cggactcagt gattgacgtc ggtttgcgta cagaacctaa ccttgaactg
    13501 ctgaccgaaa tgaaaccgtc gtttatggtc tggtcggcag gatatggccc ttcaccagaa
    13561 atgctggcac gtattgcgcc gggtcgcgga tttaacttca gtgacggcaa acagccgttg
    13621 gcgatggcgc gtaaatcgct gacggaaatg gcagatttac ttaacctgca aagcgcagca
    13681 gaaacgcatt tagcgcacta tgaagacttt atccgcagca tgaaaccccg ctttgtgaag
    13741 cgtggtgcac gcccgttact gctgacgacg cttatcgatc cgcgccatat gctggtcttc
    13801 ggtccaaaca gcttgttcca ggaaattctc gatgagtacg gcatcccaaa tgcctggcaa
    13861 ggggaaacca acttctgggg cagtaccgcc gtcagtatcg atcgtctggc ggcgtataaa
    13921 gacgttgatg tgctctgttt tgatcacgac aacagcaaag acatggatgc gctaatggca
    13981 accccgctgt ggcaggccat gccgtttgtc cgtacagggc gctttcagcg cgtacccgca
    14041 gtctggtttt atggtgcaac gctttcggca atgcactttg tgcgtgttct ggataacgcc
    14101 atcggaggta aagcgtgagt aaacgaattg cgcttttccc ggcgttattg ctggcgctgt
    14161 tagtgattgt cgctacggcg ctaacctgga tgaacttctc acaggcgctg ccgcgcagtc
    14221 agtgggcgca ggccgcctgg tcgccggata ttgacgtcat cgagcagatg atttttcact
    14281 acagcttgtt gccgcgtctg gcgatttcgc tgctggtggg cgcaggcctg gggctggtgg
    14341 gcgtgctgtt tcagcaagtg ctgcgtaatc cgctggcgga accgacgaca cttggcgttg
    14401 ctacaggcgc gcaactggga attaccgtca ccacactttg ggcgatccca ggtgctatgg
    14461 cgagccagtt tgccgcactg gtgggcgctt gtgtcgttgg cttaatcgtc tttggcgtcg
    14521 cgtgggggaa acggctttcg ccggttacgc ttattctcgc ggggctggtg gtgagccttt
    14581 attgcggggc aattaatcag ctactggtta tcttccatca cgaccagctg caaagcatgt
    14641 tcctgtggag caccggaacg ctgacgcaaa ccgactgggg cggcgttgag cgtttatggc
    14701 cgcagttgct gggcggcgtg atgttgacgt tattgctact tcgcccgtta accctgatgg
    14761 ggcttgatga tggcgtggcg cgcaatctcg ggctggcctt gtcgctcgcg cgtctggcag
    14821 cgctgtcgct ggcgattgtc atcagtgcgc tgctggtgaa cgctgtgggg attatcggct
    14881 ttatcgggtt gttcgcgccg ctgctggcaa aaatgctggg ggcgcggcgt ctgctgccac
    14941 gactgatgct ggcgtcgctg attggggcgc tgatcctctg gctttccgat caaatcatcc
    15001 tctggctgac tcgcgtgtgg atggaagtgt ccaccggttc ggtcactgcg ttgatcggtg
    15061 cgccgctgct actgtggctg ttgccgcgtt tacgcagcat tagcgcgccg gatatgaagg
    15121 tcaacgatcg tgtcgcggct gaacgccaac atgtgctggc gtttgccctc gcgggcggcg
    15181 tgctgctgtt gatggctgtg gtggtggcgc tgtcgtttgg tcgtgatgcg cacggctgga
    15241 cgtgggcgag cggggcgttg cttgaggatt tgatgccctg gcgctggccg cgaattatgg
    15301 cggcgctgtt tgcgggcgtc atgctggcgg tggcgggctg tattattcag cggctgaccg
    15361 gaaacccgat ggcaagcccg gaagtgctgg ggattagctc tggcgcagcg tttggcgtgg
    15421 tgttgatgct gtttctggtg ccgggtaatg cctttggctg gctgttacct gcaggcagtc
    15481 tcggcgcggc ggtgacgctg ttgatcatta tgatcgccgc cggccgcggt ggattttccc
    15541 cacaccgcat gttactggcg gggatggcgt taagcaccgc gttcaccatg cttttgatga
    15601 tgttgcaggc aagtggtgac ccgcgaatgg cgcaagtgct gacctggatt tccggttcga
    15661 cctacaacgc gaccgatgcg caggtctggc gcacgggaat tgtgatggtg attttgctgg
    15721 cgattacccc gctgtgccgc cgctggctga ccattttacc gctgggtggt gataccgccc
    15781 gagccgtagg aatggcgctg acgccgacgc gaattgcgct gctgctgtta gcggcttgcc
    15841 tgacggcgac cgcgacgatg actattggac cgttgagttt tgttggttta atggcaccgc
    15901 atattgcgcg gatgatgggc tttcgacgga cgatgccaca catcgtaatt tcggcgctgg
    15961 tgggtggttt actgctggtg ttcgctgact ggtgtgggcg gatggtgctg tttccattcc
    16021 agatcccagc agggctgctg tcgaccttta tcggcgcgcc atattttatc tatttgttga
    16081 gaaagcagag ccgttaatag aatgcctcgc ccggtgggcg aggcaaaatc atcacaactt
    16141 cgcaaacacc cgtcgtgcag catcgatggt gttattgata tcttccatgc tgtgcgccac
    16201 ggacataaag cccgcttcaa acgctgacgg tgccaggtaa acaccttcgt ccagcatcat
    16261 atggaagaaa cgcttaaagc gttccacgtc acaggccatc acatcctgat agcacgtcac
    16321 ggactcggcg tcggtaaaga aaataccgaa catgccgcca acgtggttaa cgaccagcgg
    16381 aattccggct tcttctgccg cttccagcag accttctgcc agacgtgttg tcagctcatc
    16441 cagcgtttcg tgaacgcccg gctgcgcgac ttcattcaga caggcgaaac ccgctgccat
    16501 cgcaattggg ttaccggaaa gcgtacccgc ctgatagacc ggacccgtcg gggccagcgc
    16561 atccattaca tcacgacgac caccgaatgc gcctaccggc attccaccgc cgatgatttt
    16621 gcccaggcag gtgagatccg gttccacacc gtaataatcc tgtgcgccag ctagcgctac
    16681 gcggaagcca gtcattactt catcgatgat cagcaatgcg ccaaattcgt cgcacagcgc
    16741 gcgcagacct ggcaggaact ctggcagcgg tggaacgcag ttcatattgc ctgccaccgg
    16801 ctcgacgata atacaggcaa tctcttgcgg gtattgctca aacgcggcgc gtacagaagc
    16861 cagatcgtta taagtacagg ttaaggtatg tttggcgaaa tctgccggaa cacccggcga
    16921 gtttggctgg cctaacgtga gtgcgccaga accggctttc accagcaggc agtcagcgtg
    16981 accgtggtaa caaccttcaa atttaatgat tttgtcgcga ccggtaaaac cacgggccag
    17041 gcggatggcg ctcatcgtcg cttcagtgcc ggagttcacc atgcgcacca tatccatggt
    17101 cgggaccagt tcggtcacca gttgcgccat tttcacttcc atttcggtcg gtgcaccaaa
    17161 gcttaaacca cgctcggcgg cttcaatcac ggcattgcgg atagccggat ggttatggcc
    17221 cagcaccatc ggcccccagg aaccgacata atcgatatag gctttgccat caacatcgta
    17281 cagataagcg ccgtccgctt tttcgataaa cagtggagtg ccgcccacgc cagtaaaggc
    17341 gcgaacaggg gagttcacac cgccagggat cagctcgcgc gctgcgctgt aaagattttc
    17401 agacttactc atggagggtt cctgattcgt agaaaaagtg aatggctgct attctatgtt
    17461 attcataaca agttaaatac tcgtcaaaca tcaggctgct tgtactggtg caatcctgat
    17521 ttcttagagt ataaaagttt tgtgcatttg aaacattacg ctttgcaaag gattttcatg
    17581 gaacgtgcga gtaaaatgcc gtcatcttat ttgtatgacc aataagtgat cattggatga
    17641 aaactgatac tccctcttta gaaacaccgc aggccgcgcg cctgcgacgc agacaactga
    17701 ttcgccaact tcttgagcgc gataaaaccc cgttagccat tttgtttatg gcggcagtcg
    17761 tcggcacgct tgtcgggctg gcagcggttg cttttgacaa aggtgtcgcc tggttgcaga
    17821 accaacgtat gggggcgctg gtacatactg ctgataatta tccgcttctg ttaaccgtcg
    17881 cttttctctg ttcggcggtg ctggcgatgt tcggctactt tttggtgcgc aaatacgcgc
    17941 cggaagcagg tggttcgggg atcccggaaa ttgaaggggc gctggaagat caacgtcccg
    18001 ttcgctggtg gcgtgtgttg ccggtgaaat tctttggcgg gctggggaca ctcggcggag
    18061 gcatggtgct agggcgcgaa ggaccaaccg tgcagatcgg cggcaatatc ggtcgcatgg
    18121 tgcttgatat tttccgcctg aaaggtgatg gagctcgcca tacgctgctg gcaaccggtg
    18181 ctgctgcggg gctggctgcg gcctttaacg cgccgctggc gggtattttg tttattatcg
    18241 aagagatgcg tccgcagttt cgctatacgt taatttcgat taaagcggta tttattggcg
    18301 tcattatgtc gaccattatg taccggattt ttaatcatga agttgcgttg attgacgtcg
    18361 gtaaactttc tgatgcgccg cttaatacgc tgtggcttta tctgatcctc ggtattattt
    18421 ttggcatttt cggccctatt tttaataaat gggtgctggg gatgcaggat ttgctgcacc
    18481 gtgtgcacgg cggcaatatt accaaatggg tactgatggg tggtgcgatt ggcggtctgt
    18541 gtggattgct ggggtttgtg gcaccagcaa cgtcgggcgg cggttttaac ctgattccta
    18601 tcgctaccgc ggggaatttc agcatgggaa tgctggtgtt tattttcgtc gctcgggtca
    18661 ttaccacctt actctgcttc tcttccggcg cgccgggcgg tatttttgcc ccgatgctgg
    18721 cgctgggtac tgtgctggga accgctttcg gaatggttgc cgttgagctg tttccgcaat
    18781 atcacctgga ggcggggacg tttgctattg ccggaatggg ggcattactg gcggcatcta
    18841 ttcgcgcgcc gttaacgggg atcattctgg ttctggagat gaccgataac taccagctca
    18901 ttttgccaat gattattacc ggtcttggcg caacactatt agcgcaattt accggcggga
    18961 aaccgctata ctcggcgatt cttgcgcgca cgctggcaaa acaggaagct atgcaactgg
    19021 cgcgaagcaa ggccgcatca gccagcgaga atacttgaac gaaataccag ggtattagat
    19081 aatggcgatt attattgggt tagaatttgc ccaattgccg atgtcgtttg gagcaaaata
    19141 tgagtgatga cgtagcactg ccgctggagt ttaccgacgc agcagccaac aaagttaaaa
    19201 gcctgatcgc tgacgaagat aacccgaatc tgaaattacg cgtgtatatc accggtggcg
    19261 gttgcagcgg cttccagtat ggtttcacct ttgatgatca ggtgaacgaa ggcgatatga
    19321 ccatcgaaaa acagggcgtt ggcttggtgg ttgatccgat gagcctgcaa tatctggtcg
    19381 gcggttccgt tgactatacc gaaggactgg aaggttctcg tttcatcgtg accaacccga
    19441 acgcgaaaag cacctgcggt tgcggttctt cctttagcat ctaatctgtt gttcatgtgc
    19501 cggataaact atccggcaca tcttctctct tcaacgccca ttctcatcca gcgcaaacgt
    19561 cggtagctta agatgccaac gaatagccgc cagccgaatc aagagcgtca cgaccatgcc
    19621 catcatactg gctgtttcca gtggtacgga aaatgtgtaa taagccgtag cgtggacaat
    19681 accgccgata atacaggcag ttgcgtagat ttctgtacgt aaaatcatgg ggatttcgcg
    19741 ggccagaaca tcacgaatga tcccgccgcc aacgccagta atgacgccca tacaaaccgc
    19801 gattaacgga ccggcttccg cattaaaggc tttattcacg ccaatgccga caaacaccgc
    19861 cagaccaacg gcgtccagca ccggcaacat ccattttggt aagcgtcttg gctggcgcac
    19921 cagcacgatg gtcagcatgc tggttaccat tgcaacgacc agatcggtgg gatctttcac
    19981 ccaaaatacc gggccgtgat ccagcgccat gtcgcgaatt gtcccgccgc ctacagcggt
    20041 aaccacgccc agtaccagaa caccaaaagg gtccatacgc aattttccgg ctaacaaaac
    20101 gccggagatg gcaaataccg ctgtgccgac tatatccagc caatagacga gcattgttca
    20161 atccccactg agcacctgaa aaggtcaggc actaatctac ctgtgaaagc gcattacaga
    20221 gctgttgtgc agcgaggata atacgtgggc ttgcacgttc aaaccagtca ctcgtgagag
    20281 gaataacggg aattttgagc tgttcacccc agtattgttt gattttagga atttggtccg
    20341 gtccgcctgt aatgacaatc gcctgtggcg agcgtgctaa cacctgttcg cggctaactt
    20401 gcggccaggg aacccggctg tctttaaaga tgttttctcc gccacaaact tcgagtacct
    20461 ggttctgaat cgactctttt ccactggtaa atggcggatt aatgccgaat tgcagaaaaa
    20521 cacgtttttt aggtttatca gcatattgcg ctttcaattg cgcgtactga tccagcaggg
    20581 attgcgcggc ttgttcggcc ttgtctggtt gcggactcca gggggccagt tgacgtaacg
    20641 cattggcaat ttgttcaatg cttgtcgcat cgacccacat cacttttatt cccagcgaag
    20701 ccagctggtc aacctgccgc tcggcattac ctccacgcca ggcaatcacc agatcgggtt
    20761 tcagcgcgac aatgcgttcc agattcatcc cctgccaggt ggaaacctgc tcaatctttt
    20821 gcgcttgtgg aggatagtcg gaatagctgc tgaccccaac cggcgtgatc ccggcggcaa
    20881 aggcaagttc agtgttggcg ggagaaagcg tgatgacgcg cggcgcggcg ttgagccaca
    20941 gtggcgcaag aaaagacagg gcgaccagcg ccctgaacag tgacttagcc atgtgcaagt
    21001 ttctgcacca gtgactcaac catcaggctg gactgtttag cggcaacagc caggaactca
    21061 tcgaagctaa gatgagactg ttgatcggcc acgtcggaga tggcgcgtac gacaacaaac
    21121 gggacgttga aattgtggca gacatgggcg attgccgtcg cttccatctc tacagcaatg
    21181 gcctgtggga agttgtggcg gattttcgcc agaccaacag aaccgttgat gaaagcgtcg
    21241 ccgctaacaa tcaggccacg tacagcgtta agattcagtt cggcaatgca ggcctcagcg
    21301 gcagcgatca gtttatcgtc agctttaaag cctgccggac agcctggtaa ctgaccgtat
    21361 tcataaccaa atgccgtgac atccgcgtcg tgataacgtg cttcgtccga gacaacgata
    21421 tcgcccactt tcaacgttgg tgccaggcca ccggcagaac cggtgttaat aatcacatct
    21481 ggcttgcagt gttccaacag caaagtggca cccagcgccg cagcgacttt accgatgccc
    21541 gatttcagaa gcgcaacctc ggttccattc agttggccgg tatagatttc gcaaccgccg
    21601 agactgatag tttgacgttt ttcgattttg tcacgcagca gcgtaacttc ttcttccatt
    21661 gcaccaatga tgccgatttt catagattta ctcgcgataa gcccgatttg aaggcatagt
    21721 ttaccatgcg cttacgggga agcgtatttc tcacgcggga gaggacatgg cacagattga
    21781 tttccgaaaa aaaataaact ggcatcgtcg ttaccgttca ccgcagggcg ttaaaaccga
    21841 acatgagatc ctgcggatct tcgagagcga tcgcgggcgt atcatcaact ctccggcaat
    21901 tcgtcgtctg caacaaaaga cgcaggtttt tccactggag cgcaatgccg ccgtgcgcac
    21961 gcgtcttacc cactcgatgg aagtccagca ggtggggcgc tacatcgcca aagaaatttt
    22021 aagccgtctg aaagagctta aattgctgga agcatacggc ctggatgaac tgaccggacc
    22081 ttttgaaagc attgttgaga tgtcatgcct gatgcacgat atcggcaatc cgccgtttgg
    22141 tcattttggc gaagcagcga taaatgactg gtttcgccaa cgtttgcacc cggaagatgc
    22201 cgaaagccag cctctgactg acgatcgctg cagcgtggcg gcactacgtt tacgggacgg
    22261 ggaagaaccg cttaacgagc tgcggcgcaa gattcgtcag gacttatgtc attttgaggg
    22321 gaatgcacaa ggcattcgtc tggtgcatac attgatgcgg atgaatctca cctgggcaca
    22381 ggttggcggt attttaaaat atacccgtcc ggcgtggtgg cgtggcgaaa cgcctgagac
    22441 acatcactat ttaatgaaaa agccgggtta ttatctttct gaagaagcct atattgcccg
    22501 gttgcgtaaa gaacttaatt tggcgcttta cagtcgtttt ccattaactt ggattatgga
    22561 agctgccgac gacatctcct attgtgtggc agaccttgaa gatgcggtag agaaaagaat
    22621 atttaccgtt gagcagcttt atcatcattt gcacgaagcg tggggccagc atgagaaagg
    22681 ttcgctcttt tcgctggtgg ttgaaaatgc ctgggaaaaa tcacgctcaa atagtttaag
    22741 ccgcagtacg gaagatcagt tttttatgta tttacgggta aacaccctaa ataaactggt
    22801 accctacgcg gcacaacgat ttattgataa tctgcctgcg attttcgccg gaacgtttaa
    22861 tcatgcatta ttggaagatg ccagcgaatg cagcgatctt cttaagctat ataaaaatgt
    22921 cgctgtaaaa catgtgttta gccatccaga tgtcgagcag cttgaattgc agggctatcg
    22981 ggtcattagc ggattattag agatttatcg tcctttatta agcctgtcgt tatcagactt
    23041 tactgaactg gtagaaaaag aacgggtgaa acgtttccct attgaatcgc gcttattcca
    23101 caaactctcg acgcgccatc ggctggccta tgtcgaggct gtcagtaaat taccgtcaga
    23161 ttctcctgag tttccgctat gggaatatta ttaccgttgc cgcctgctgc aggattatat
    23221 cagcggtatg accgacctct atgcgtggga tgaataccga cgtctgatgg ccgtagaaca
    23281 ataaccaggc ttttgtaaag acgaacaata aatttttacc ttttgcagaa actttagttc
    23341 ggaacttcgc gctataaaac gaatctgaag aacacagcaa ttttgcgtta tctgttaatc
    23401 gagactgaaa tacatgaaaa aaaccacatt agcactgagt gcactggctc tgagtttagg
    23461 tttggcgtta tctccgctct ctgcaacggc ggctgagact tcttcagcaa cgacagccca
    23521 gcagatgcca agccttgcac cgatgctcga aaaggtgatg ccttcagtgg tcagcattaa
    23581 cgtagaaggt agcacaaccg ttaatacgcc gcgtatgccg cgtaatttcc agcagttctt
    23641 cggtgatgat tctccgttct gccaggaagg ttcaccgttc cagagctctc cgttctgcca
    23701 ggggggccag ggcggtaatg gcggcggcca gcaacagaaa ttcatggcgc tgggttccgg
    23761 cgtcatcatt gatgccgata aaggctatgt cgtcaccaac aaccacgttg ttgataacgc
    23821 gacggtgatt aaagtccagc tgggcgatgg tcgtaagttc gacgcgaaga tggttggcaa
    23881 agatccgcgc tctgatatcg cgctgattca gatccagaac ccgaaaaacc tgaccgcaat
    23941 taagatggcg gattctgatg cactgcgcgt gggtgattac accgtagcga ttggtaaccc
    24001 gtttggcctg ggagagacgg taacttccgg gattgtctct gcgctggggc gtagcggcct
    24061 gaatgccgaa aactacgaaa acttcatcca gaccgatgca gcgatcaacc gtggtaactc
    24121 cggtggtgcg ctggttaacc tgaacggcga actgatcggt atcaacaccg cgatcctcgc
    24181 accggacggc ggcaacatcg gtatcggttt tgctatcccg agcaacatgg tgaaaaacct
    24241 gacctcgcag atggtggaat acggccaggt gaaacgcggt gagctgggta ttatggggac
    24301 tgagctgaat tccgaactgg cgaaagcgat gaaagttgac gcccagcgcg gtgctttcgt
    24361 aagccaggtt ctgcctaatt cctccgctgc aaaagcgggc attaaagcgg gtgatgtgat
    24421 cacctcactg aacggtaagc caatcagcag ctttgccgca ctgcgtgctc aggtgggtac
    24481 tatgccggtg ggcagcaaac tgaccctggg cttgctgcgc gacggtaagc aggtcaacgt
    24541 gaatctggaa ctgcagcaga gcagccagaa tcaggttgat tccagctcca tcttcaacgg
    24601 cattgaaggc gctgagatga gcaacaaagg caaagatcag ggcgtggtag tgaacaacgt
    24661 gaaaacgggc actccggctg cgcagatcgg cctgaagaaa ggtgatgtga ttattggcgc
    24721 gaaccagcag gcagtgaaaa acatcgctga actgcgtaaa gttcttgaca gcaaaccgtc
    24781 tgtactggca ctgaacattc agcgcggcga cagctccatc tacctgttaa tgcagtaatc
    24841 tccctcaacc ccttcctgaa aacgggaagg ggttctcctt acaatctgtg aacttcacca
    24901 caactccata catcttcatc atcctttagg catttgcaca atgccgtacg ttacgtactt
    24961 ccttatgcta agccgtgcat aacggaggac ttatggctgg catcttgata ccaaaatggc
    25021 gcaggatatc gtggcacgta ccatgcgcat catcgatacc aatatcaacg taatggatgc
    25081 ccgtgggcga attatcggca gcggcgatcg tgagcgtatt ggtgaattgc acgaaggtgc
    25141 attgctggta ctttcacagg gacgagtcgt cgatatcgat gacgcggtgg cacgtcatct
    25201 gcacggtgtg cggcaaggga ttaatctacc gttacggctg gaaggtgaaa ttgtcggcgt
    25261 aattggcctg acaggtgaac cagagaatct gcgtaaatat ggcgaactgg tctgcatgac
    25321 ggctgaaatg atgctggaac agtcgcggtt gatgcacttg ttggctcagg atagccgttt
    25381 gcgggaggaa ctggtgatga acctgattca ggcagaggag aatactcccg cacttactga
    25441 atgggcgcaa cggctgggga tcgatctcaa tcaaccgcga gtggtggcta ttgttgaggt
    25501 cgacagcggt cagcttggtg tggacagcgc aatggcggag ttacaacaac tgcaaaacgc
    25561 gctgactacg cccgagcgta ataatctggt ggcgattgtc tcgctaaccg aaatggtggt
    25621 gttgaaaccg gcgttgaact cttttgggcg ctgggatgca gaagatcatc gtaagcgagt
    25681 tgaacaactg attacccgca tgaaagagta cggccagctg cgttttcgcg tttcactggg
    25741 caactatttt accggtcctg gcagtattgc ccgatcctat cgtacggcga aaacgacgat
    25801 ggtggtgggt aaacagcgga tgccagaaag tcgctgctat ttttatcagg atctgatgtt
    25861 acctgtgtta ctcgacagtt tgcgtggcga ctggcaggcc aacgaactgg cgcgaccgct
    25921 ggcgcggctg aaagcgatgg acaataacgg cttgctgcga cgaacgctgg cggcgtggtt
    25981 tcgtcacaat gtgcaaccgc tggcaacgtc aaaggcgttg tttattcatc gtaataccct
    26041 ggagtatcgg cttaatcgta tatcggaact gaccgggctt gatttgggca attttgatga
    26101 caggttgctg ctgtatgtgg cgttacaact ggatgaagag cggtaggtta tgcgttaagg
    26161 ttgaggcggc gctggctcat tcccctcagc ccgaagggga gaggggaatg cccgaattaa
    26221 ccgtcaattc gggattattt acgcgttagt ttttccagat cggcttcaat ctcgctgatc
    26281 ttattggtta cgactgactc caggtgacgt aagtcgtcga ggatcttacg cttaagatca
    26341 acttcgctgc ggtcacgctg gcagatttga tcaagctcat caatgatata ccgtagattc
    26401 gggctgattt cctggacttc tttataaccc tgacccacac catcagcgac gaccgtttta
    26461 cgctgacgcg gatatttaaa cttaacgctc ttggcgaaaa actcgccttt gtctttctgg
    26521 aaatagattt tcagaatatc gttgttggct tcctgccgga ggctgtaacg atcaatttct
    26581 tcaggattgg taatacccag acttttcaga ttgtcgtaca tagcgttacc tcaaaatgag
    26641 tcagtaaatt gtgcttattt tagcatttgg cctggcccgc ccggctcgac tgtgatcgca
    26701 gaaagccttt ttcttccaga cttctatcat ggcgtaaaaa tcaaaaaatt acctgcttta
    26761 ttgtggtgat aaaattcacg ttctacacca gttccagcat cgttacttcc gggcggcagt
    26821 tcagacgcaa accatacaaa ctgcccacgc cacgggttgt gtagatgtgt cgttcgccaa
    26881 aggcatttaa tccggcgacg taacgtttat cttcgacagg ggcaaaaggt tcaccaacca
    26941 acggtacgcg cagttgcccg ccgtgagtat ggccgcacag catcagatcc cagggttcat
    27001 cacgcatgac ttctttgctg tcgggattat gcgccagcac cagtcttggc agattggctt
    27061 cgctggcggg aggcggtttg cattgtccgg cccataagtc accggtgcct accagttcga
    27121 attgcctgtt cggcgtggcg atcaccgtag cctggttaaa caacaccgtg atgcccgctg
    27181 atttcaacgt ctcgccaatt aagtgatttt tttctgtacc aacagggcga tcgtggttgc
    27241 cgaagcaggc aaacgtcggc gcacattcgg caagggggga gaggacatca ctaaacgccg
    27301 aaaaattcag cggcatatca aataatacgt aatcgccgcc cagtaatatc aaatcgggct
    27361 tttgttctat gccaagagca atcgcgtcag aaatcaggct taaaggaaca aaacgagagt
    27421 aatggagatc ggccagaaaa agaattttga atggtgctgc gttgtcttta aaaaaggcga
    27481 ggcggtgacg gattaattca aaccagcctg gctcccagta atgcatataa ccgaaacctg
    27541 agctcgtggc tatcgtcgcg gcagtagcct gcaacaatcg gcggcgtgaa atcatcgctc
    27601 atccctgcaa aaaaaatcgg gcagcgtcgt gctgcccgtg tgcatacttt tagtcgatgg
    27661 tacgcagcag ttcgttaatg ccgactttgc cgcgagtttt cgcgtcaact ttcttaacga
    27721 taaccgcaca gtagaggctg tatttgccat cttttgacgg cagattacct gaaacaacca
    27781 cagaccccgc cggaacgcga ccgtagtgga tttcgccggt ttcacggtcg taaatacggg
    27841 tgctctgacc aatgtatacg cccatggaaa tgacggaacc ttcttcgaca atcacccctt
    27901 caaccacttc agagcgtgcg ccaatgaagc agttatcttc aatgatggtt gggttagcct
    27961 gcagcggttc cagcacgccg ccgatgccca cgccaccgga aaggtggacg tttttaccaa
    28021 tctgcgcaca agaaccgacg gtcgcccagg tatcaaccat ggtgccttca tcaacatatg
    28081 cgccgatgtt gacgtaagac ggcatcagca cggtgttacg ggcaataaac gcaccctgac
    28141 gtaccgccgc tggtggcaca acgcggaagc cttctttctg gaaacgtgct tcgtcgtagt
    28201 cggcgaattt catcggcact ttgtcgaagt agcggctttc tgccccttcg atcacctgat
    28261 tatcattaat acggaaagag agcagcaccg cttttttcaa ccactgatgc gtcacccact
    28321 gaccgtcaat tttttccgct acacgcagtg caccggaatc cagcagggcg atcacctgat
    28381 ttaccgcttc gcgggtaacg gtgtctgcgt tggctggcgt gatctcggca cggcgttcaa
    28441 aagcggtttc aataatgttc tgtaactgct gcattgttaa actcttttca tatcagtaaa
    28501 cacatcaccc tttatcgttt ggattgaggg cctctgtcaa ccgctgatgc acttcctgct
    28561 gcaactcgtt attaagtgca cgccggtcag cggtggcaat tatgaataaa tcttctactc
    28621 gctcgccaat ggttgtaatt cgggcaccat gaagcgaaat tcccagatcg gcaaaaattt
    28681 tcccgactct cgccagcagt cctggctggt cgagcgcgat aagctcaagg aacgatttgc
    28741 ggtcggtatg ggtcggcaaa aacgttactt cggtttcaac agtaaaatgg cgtaatttgg
    28801 cgggttggcg acggggctgc ggtggctgcc agctactttg cgtcagtact tgctccagac
    28861 caaaccgaat aacctcatga cgatctgcgg acagcgggct gccatcgggt tccagcacga
    28921 taaaggtatc catcgccata ccgtcgcgag tggtgaaaat ttgtgcgtcg tgaacactta
    28981 aattgcggcg gtctaattcg gcacagacgg cggcaaacag ataggggcgg tccgggctcc
    29041 agataaaaat ctcggtgcct ccacgcgtag cctgcgggct aagcaatacc agcggtttgc
    29101 ttaaatcatg ctgtaataaa tggcgggcat gccaggccag ttgatttggg ctatggcgga
    29161 caaaatagtt agcacgacag cgtgaccaaa tttggtgcag cgcctcttcg tcgatgttat
    29221 ccatgcgcag tagtgccagt gcctggagtt ggtgatgacg aacccgttcg cgcatatccg
    29281 gcgtgttttg cattccgcgt cgtagctgct tttcggtggc aaaatagagt tcgcgcaaca
    29341 gactttgctt ccagctattc cacagcgttt cgttggtggc gcaaatgtca gccaccgtca
    29401 ggcataccag ataacgaagt cggttttctg tttgcacttc ttcggcaaac tgcttgatga
    29461 cttccgggtc ctgaatatcg cggcgttggg cggtcaccga catcaacagg tgctggcgaa
    29521 ccagccaggc aaccagctgt gtttcgcgcg agttaagccc gtggagttcg gcaaaatgca
    29581 ctacatcctg agcaccgaga atggagtggt cgccgccgcg tcctttggcg atatcgtgaa
    29641 acagcgcggc gatgaaaatc agctcagttg acggcaggcg cggccagacg tccacacaca
    29701 acggatggcg ctggcgcgtt tcttcactgg caaaactctc cagtttcagc atcacgcgga
    29761 tagtatgttc atccaccgtg taggcgtgga acagatcaaa ctgcatctgc ccgacgatat
    29821 gcgaccattg cggcatatac gcgccgagca cgctatggcg atgcattggc aatagcccgc
    29881 gccgcaccgc tccggggtga cgcagaatgc tcaaaaacag ttttcgtgct tccggaatat
    29941 tacacagcgg ttgttgcaga tggcgacggg catggcgtaa ctggcgcagc gtggtggagt
    30001 aaatgccggt gatcgcactg ttgcgcacca tggtgtagaa catacgcaag atggcttccg
    30061 gctggcgcat aaatagtgtt tcatcacgca ggtcgattag cgtaccgcgt agctgaaact
    30121 catcgtcgag tggacgtggt ttttcgtcgg cgggaagggc gaggatggct tcatcgaaca
    30181 gttgcagcag catctggttg agttcactga cgcggcgtgt aacgcggaag taatccttca
    30241 tcatccgctc gaccggttcg ttgccttcac cactgtaatt cagacgctga gcgacgctaa
    30301 gttggcgatc gaataacaga cgattatcgt aacggctgac gaccagatgc agggcaaagc
    30361 gaatacgcca cagtatatgc agacattcgt ttaattccgc ccgctccgct gaggttaaga
    30421 agccaaaccc aaccatttca tccagcgatg ttgcgccaaa atgacggcgg gccacccatt
    30481 gcagagtgtg gatatcgcgc aagccgccag ggctactttt gatgtctggt tcaagattgt
    30541 agctggtgcc gtggtaacgc tgatggcgct ggttctgttc ttcaactttc gccgcgtaga
    30601 acttgtcgga aggccagaat ccttcgctaa aaatatgttt ttgcagttcg aggaagagcg
    30661 caacatcgcc aattaataag cgggattcga ttaaattggt ggcgacggtt aaatccgata
    30721 acccttccag catgcactct tcaagcgtgc gcacactatg accgacttcc agctttacat
    30781 cccagagcag cgttaacagc tcgcccactt tttgcgcctg atcgtccggg agctttttac
    30841 ggcttaaaat cagtaaatcg atgtcagaaa gtggatgcag ctcgccacga ccgtagccac
    30901 cgacggcgac caatgccagg tcggcaatct ggctgaatcc cgcttcaatc cataatcgtt
    30961 gcaggagctg gtcgataaac tcggtgcgcg cctcaatcaa ctgttctgca gagatcccat
    31021 tgtcaaaggc atcacccagc caacgctgga aagtatcgat atgggctttt atcccaccga
    31081 cggttaattc atcacggggc cagacgcatg gattttgcag ttgaccgggc agggtgggga
    31141 gagcggtgtt tgcgtactgt tctggaaggg tattcattgt gcgccaccca taagattaaa
    31201 ttatcgcatt aaaaaagccg gcattcgccg gcttcatctt attcgtcgtg cgagattatc
    31261 gccgggatgg tgtcatcctt gcgtagcgtc agaatttcgc agccgttatc agtcaccaca
    31321 atagtatgct catattgtgc agacaagctg cgatctttgg tttttaccgt ccagccatct
    31381 ttcatggtgc ggatctcttt tttacccgcg ttgaccattg gctcgatggt gaacgtcatc
    31441 ccaggtttca gtacgacgtt ggtttcacgg gagtcatagt gcagcacctg cggttcttca
    31501 tggaagccgc gaccaatacc gtgtccgcaa tattcacgaa cgacggagaa gccttctgct
    31561 tcgacaaatt tctgaatcgc cgcaccgatt tcgcgcagat taatgcctgg tttgaccatg
    31621 cgcagcgcca ggtacaggct ttcttgcgtg atgcggcaca gacgttcgcc catgatggtc
    31681 ggcttaccga cgataaacat tttcgaggta tcgccgtgga aaccatcttt gattacggtg
    31741 acatcaatgt taacgatatc gccatctttc agcagcttcg catcgtccgg gataccgtgg
    31801 cacaccactt cattaataga gatgcaaacg gatttcggat agccgtgata gccgaggcag
    31861 gcagaaaccg tgtgttgttc attaacaatg taatcattac agatgcgatc cagctcgccg
    31921 gtgctgacgc ccggtttaac atacggttcg atcatctcca gcacttcggc agccagtcgg
    31981 ccagcgacgc gcattttttc gatatcttct ggggtcttga ttgagatagc cattaattct
    32041 gtccatcagc gtcggtgata ccgacaatat atatgtaagt gccgtcaatg gtatcacacc
    32101 cgggcaaatt gagaatcatt ctgaatttcg ccaaacgtgc cactgaacgt tttctataat
    32161 agaaaattca acgtctgatg ctgtacacag cgccaacaat tattggtgtc catgacgtat
    32221 ttgtggtata aagcgcgccg gacttccgat ccatttcgta tacacagact ggacggaagc
    32281 gacaatctca ctttgtgtaa caacacacac gtatcggcac atattccggg gtgccctttg
    32341 gggtcggtaa tatgggatac gtggaggcat aaccccaact tttatataga ggttttaatc
    32401 atggcaactg tttccatgcg cgacatgctc aaggctggtg ttcacttcgg tcaccagacc
    32461 cgttactgga acccgaaaat gaagccgttc atcttcggtg cgcgtaacaa agttcacatc
    32521 atcaaccttg agaaaactgt accgatgttc aacgaagctc tggctgaact gaacaagatt
    32581 gcttctcgca aaggtaaaat ccttttcgtt ggtactaaac gcgctgcaag cgaagcggtg
    32641 aaagacgctg ctctgagctg cgaccagttc ttcgtgaacc atcgctggct gggcggtatg
    32701 ctgactaact ggaaaaccgt tcgtcagtcc atcaaacgtc tgaaagacct ggaaactcag
    32761 tctcaggacg gtactttcga aaagctgacc aagaaagaag cgctgatgcg cactcgtgag
    32821 ctggagaaac tggaaaacag cctgggcggt atcaaagaca tgggcggtct gccggacgct
    32881 ctgtttgtaa tcgatgctga ccacgaacac attgctatca aagaagcaaa caacctgggt
    32941 attccggtat ttgctatcgt tgataccaac tctgatccgg acggtgttga cttcgttatc
    33001 ccgggtaacg acgacgcaat ccgtgctgtg accctgtacc tgggcgctgt tgctgcaacc
    33061 gtacgtgaag gccgttctca ggatctggct tcccaggcgg aagaaagctt cgtagaagct
    33121 gagtaataag gcttgataac tcccccaaaa tagttcgagt tgcagaaagg cggcaagctc
    33181 gagaattccc gggagcttac atcagtaagt gaccgggatg agcgagcgaa gacaacgcat
    33241 ctgcggcgcg aaatatgaag ggggagagcc cttatagacc aggtagtaca cgtttggtta
    33301 gggggcctgc atatggcccc ctttttcact tttatatctg tgcggtttaa tgccgggcag
    33361 atcacatctc cgaggatttt agaatggctg aaattaccgc atccctggta aaagagctgc
    33421 gtgagcgtac tggcgcaggc atgatggatt gcaaaaaagc actgactgaa gctaacggcg
    33481 acatcgagct ggcaatcgaa aacatgcgta agtccggtgc tattaaagca gcgaaaaaag
    33541 caggcaacgt tgctgctgac ggcgtgatca aaaccaaaat cgacggcaac tacggcatca
    33601 ttctggaagt taactgccag actgacttcg ttgcaaaaga cgctggtttc caggcgttcg
    33661 cagacaaagt tctggacgca gctgttgctg gcaaaatcac tgacgttgaa gttctgaaag
    33721 cacagttcga agaagaacgt gttgcgctgg tagcgaaaat tggtgagaac atcaacattc
    33781 gccgcgttgc tgcgctggaa ggcgacgttc tgggttctta tcagcacggt gcgcgtatcg
    33841 gcgttctggt tgctgctaaa ggcgctgacg aagagctggt taaacacatc gctatgcacg
    33901 ttgctgcaag caagccagaa ttcatcaaac cagaagacgt atccgctgaa gtggtagaaa
    33961 aagaatacca ggtacagctg gatatcgcga tgcagtctgg taagccgaaa gaaatcgcag
    34021 agaaaatggt tgaaggccgc atgaagaaat tcaccggcga agtttctctg accggtcagc
    34081 cgttcgttat ggaaccaagc aaaactgttg gtcagctgct gaaagagcat aacgctgaag
    34141 tgactggctt catccgcttc gaagtgggtg aaggcatcga gaaagttgag actgactttg
    34201 cagcagaagt tgctgcgatg tccaagcagt cttaattatc aaaaaggagc cgcctgaggg
    34261 cggcttcttt ttgtgcccat cttgtaaatt cagctaaccc ttgtggggct gcgctgaaaa
    34321 gcgacgtaca atgtcgctag tattaattca tttcaatcgt tgacagtctc aggaaagaaa
    34381 catggctacc aatgcaaaac ccgtctataa acgcattctg cttaagttga gtggcgaagc
    34441 tctgcagggc actgaaggct tcggtattga tgcaagcata ctggatcgta tggctcagga
    34501 aatcaaagaa ctggttgaac tgggtattca ggttggtgtg gtgattggtg ggggtaacct
    34561 gttccgtggc gctggtctgg cgaaagcggg tatgaaccgc gttgtgggcg accacatggg
    34621 gatgctggcg accgtaatga acggcctggc aatgcgtgat gcactgcacc gcgcctatgt
    34681 gaacgctcgt ctgatgtccg ctattccatt gaatggcgtg tgcgacagct acagctgggc
    34741 agaagctatc agcctgttgc gcaacaaccg tgtggtgatc ctctctgccg gtacaggtaa
    34801 cccgttcttt accaccgact cagcagcttg cctgcgtggt atcgaaattg aagccgatgt
    34861 ggtgctgaaa gcaaccaaag ttgacggcgt gtttaccgct gatccggcga aagatccaac
    34921 cgcaaccatg tacgagcaac tgacttacag cgaagtgctg gaaaaagagc tgaaagtcat
    34981 ggacctagcg gccttcacgc tggctcgtga ccataaatta ccgattcgtg ttttcaatat
    35041 gaacaaaccg ggtgcgctgc gccgtgtggt aatgggtgaa aaagaaggga ctttaatcac
    35101 ggaataattc ccgtgatgga taaataaggg taagattccg cgtaagtatc gcgggggcgt
    35161 tagtctggtt ataaggcgtt attgttgcag gcagtttggt cacggccagc gcgcagcaac
    35221 cggagcgtac aaaagtacgt gaggatggcg agcactgctc ggggccaaaa tggcaaataa
    35281 aatagcctaa taatccagac gattacccgt aatatgttta atcagggcta tacttagcac
    35341 acttccactg tgtgtgactg tctggtctga ctgagacaag ttttcaagga ttcgtaacgt
    35401 gattagcgat atcagaaaag atgctgaagt acgcatggac aaatgcgtag aagcgttcaa
    35461 aacccaaatc agcaaaatac gcacgggtcg tgcttctccc agcctgctgg atggcattgt
    35521 cgtggaatat tacggcacgc cgacgccgct gcgtcagctg gcaagcgtaa cggtagaaga
    35581 ttcccgtaca ctgaaaatca acgtgtttga tcgttcaatg tctccggccg ttgaaaaagc
    35641 gattatggcg tccgatcttg gcctgaaccc gaactctgcg ggtagcgaca tccgtgttcc
    35701 gctgccgccg ctgacggaag aacgtcgtaa agatctgacc aaaatcgttc gtggtgaagc
    35761 agaacaagcg cgtgttgcag tacgtaacgt gcgtcgtgac gcgaacgaca aagtgaaagc
    35821 actgttgaaa gataaagaga tcagcgaaga cgacgatcgc cgttctcagg acgatgtaca
    35881 gaaactgact gatgctgcaa tcaagaaaat tgaagcggcg ctggcagaca aagaagcaga
    35941 actgatgcag ttctgatttc ttgaacgaca aaaacgccgc tcagtagatc cttgcggatc
    36001 ggctggcggc gttttgcttt ttattctgtc tcaactctgg atgtttcatg aagcaactca
    36061 ccattctggg ctcgaccggc tcgattggtt gcagcacgct ggacgtggtg cgccataatc
    36121 ccgaacactt ccgcgtagtt gcgctggtgg caggcaaaaa tgtcactcgc atggtagaac
    36181 agtgcctgga attctctccc cgctatgccg taatggacga tgaagcgagt gcgaaacttc
    36241 ttaaaacgat gctacagcaa cagggtagcc gcaccgaagt cttaagtggg caacaagccg
    36301 cttgcgatat ggcagcgctt gaggatgttg atcaggtgat ggcagccatt gttggcgctg
    36361 ctgggctgtt acctacgctt gctgcgatcc gcgcgggtaa aaccattttg ctggccaata
    36421 aagaatcact ggttacctgc ggacgtctgt ttatggacgc cgtaaagcag agcaaagcgc
    36481 aattgttacc ggtcgatagc gaacataacg ccatttttca gagtttaccg caacctatcc
    36541 agcataatct gggatacgct gaccttgagc aaaatggcgt ggtgtccatt ttacttaccg
    36601 ggtctggtgg ccctttccgt gagacgccat tgcgcgattt ggcaacaatg acgccggatc
    36661 aagcctgccg tcatccgaac tggtcgatgg ggcgtaaaat ttctgtcgat tcggctacca
    36721 tgatgaacaa aggtctggaa tacattgaag cgcgttggct gtttaacgcc agcgccagcc
    36781 agatggaagt gctgattcac ccgcagtcag tgattcactc aatggtgcgc tatcaggacg
    36841 gcagtgttct ggcgcagctg ggggaaccgg atatgcgtac gccaattgcc cacaccatgg
    36901 catggccgaa tcgcgtgaac tctggcgtga agccgctcga tttttgcaaa ctaagtgcgt
    36961 tgacatttgc cgcaccggat tatgatcgtt atccatgcct gaaactggcg atggaggcgt
    37021 tcgaacaagg tcaggcagcg acgacagcat tgaatgccgc aaacgaaatc accgttgctg
    37081 cttttcttgc gcaacaaatc cgctttacgg atatcgctgc gttgaattta tccgtactgg
    37141 aaaaaatgga tatgcgcgaa ccacaatgtg tggacgatgt gttatctgtt gatgcgaacg
    37201 cgcgtgaagt cgccagaaaa gaggtgatgc gtctcgcaag ctgaggataa tccggctaca
    37261 gagagtcgcg ctatttgtta gcgtagggct tcagtgatat agtctgcgcc atctgatcgt
    37321 aagtagttgg ctttataagg tcaggtatgc cgtggtttta cacggctttt ttttgtatag
    37381 gcttcagtat tcctgagtac cgtaaaccct gtcagggaat aaaaaacgcg tgatgttgtc
    37441 tgctactcaa ccacttagcg aaaaattgcc agcgcatggc tgccgtcatg ttgcgatcat
    37501 tatggacggc aatggtcgct gggcaaaaaa gcaagggaag attcgtgcct ttgggcataa
    37561 agccggggca aaatccgtcc gccgggctgt ctcttttgcg gccaacaacg gtattgaggc
    37621 gttaacgctg tatgccttta gtagtgaaaa ctggaaccga ccagcgcagg aagtcagtgc
    37681 gttaatggaa ctgtttgtgt gggcgctcga tagcgaagta aaaagtctgc accgacataa
    37741 cgtgcgtctg cgtattattg gcgataccag tcgctttaac tcgcgtttgc aagaacgtat
    37801 tcgtaaatct gaagcgctaa cagccgggaa tagcggtctg acgctgaata ttgcggcgaa
    37861 ctacggtgga cgttgggata tagtccaggg agtcaggcaa ctggctgaaa aggtgcagca
    37921 aggaaacctg caaccagatc agatagatga agagatgcta aaccagcatg tctgtatgca
    37981 tgaactggcc cctgtagatt tagtaattag gactgggggg gagcatcgca ttagtaactt
    38041 tttgctttgg caaattgcct atgccgaact ttactttaca gatgttctct ggcccgattt
    38101 cgatgaacaa gactttgaag gggcgttaaa tgcctttgct aatcgagagc gtcgtttcgg
    38161 cggcaccgag cccggtgatg aaacagcctg atgggggtcg cttttgctga agtatcgcct
    38221 gatatctgct tttgtgttaa tacccgtcgt catcgcggcg ttgtttctgt tgccgccggt
    38281 ggggttcgcc atcgtaacgc tggtggtctg catgctggca gcgtgggaat ggggacagct
    38341 tagcggtttt accactcgtt cgcagcgagt atggttggcg gtgttatgcg ggttattgtt
    38401 ggcgctgatg ctttttctgt tgccggaata tcaccgaaat attcatcaac cgctggttga
    38461 aatctcactt tgggcttcgt tgggttggtg gattgtcgcg ctattgctgg tgctgtttta
    38521 cccaggttcc gcagcaatct ggcgtaactc taaaacattg cgccttattt ttggcgtgct
    38581 aaccattgtt cccttcttct ggggcatgct ggcgttacgg gcctggcact acgacgagaa
    38641 tcattacagt ggcgcaatat ggctgctcta tgtcatgatc ctggtatggg gcgctgactc
    38701 tggcgcatat atgtttggca aattgtttgg taaacataag ctggcaccga aggtttctcc
    38761 gggtaaaacc tggcaaggct ttataggtgg actagcgacc gcagcggtaa tctcatgggg
    38821 ttatggcatg tgggcgaatc tcgacgtcgc tcccgtcacc ttactcattt gctctattgt
    38881 cgcagcgtta gcctcagtgc tcggcgatct gaccgagagt atgtttaagc gtgaagcagg
    38941 aattaaggac agcggtcatt taattccagg acacggtggt attttagatc gtattgatag
    39001 cctgacggct gcggtaccgg tctttgcttg cttgttgtta ctggtattca ggacgcttta
    39061 acggaaggta atatgctgag ttttctctgg gatttggctt cgttcatcgt tgcactgggt
    39121 gtacttatca ccgtgcatga atttggtcat ttctgggttg cccggcgttg tggtgttcgc
    39181 gttgagcgtt tctcaatagg gtttggtaag gcgctctggc ggcgaactga taagctcggc
    39241 accgaatatg ttatcgccct gatcccgctg ggcggttatg tcaaaatgct ggatgagcgc
    39301 gcagaaccgg tcgttccgga actccgccac catgccttca ataataaatc tgtcggccaa
    39361 cgagcggcga ttattgccgc aggtccggtt gcaaacttca tttttgctat ctttgcctac
    39421 tggctggttt ttattattgg tgtgcctggc gtacgtccgg tggttggtga aatagcagcc
    39481 aattcgatag ctgcggaagc acaaattgca ccaggtacgg aactaaaagc cgtagatggt
    39541 atcgaaacgc ctgattggga tgccgtgcgt ttgcagttgg tcgataaaat tggcgatgaa
    39601 agcaccacca ttacagtagc gccatttggc agcgaccaac ggcgggatgt aaagctcgat
    39661 ttacgtcact gggcgtttga gcctgataaa gaagatccgg tatcttcgct ggggattcgt
    39721 cctcgtgggc cgcaaattga acctgtactg gaaaatgtgc aaccaaactc ggcggcaagc
    39781 aaggcaggtt tgcaagcagg cgacaggatc gttaaagtcg atggtcagcc cttaacgcag
    39841 tgggtgacct ttgtgatgct tgtccgggat aacccgggta aatccttagc gttagaaatc
    39901 gaaaggcagg gaagtccctt gtctttgaca ttaatcccgg agagtaaacc gggtaatggt
    39961 aaagcgattg gttttgtcgg tattgagccg aaagtcattc ctttgccaga tgagtataaa
    40021 gttgtacgcc agtatgggcc gttcaacgcc atcgtcgaag ccacggacaa aacgtggcag
    40081 ctgatgaagc tgacggtcag tatgctggga aaattgatca ccggtgatgt gaaactgaac
    40141 aacctcagtg ggccgatctc tatcgccaag ggggctggga tgacagcgga actcggggta
    40201 gtttattacc tgccgtttct tgcgcttatt agcgtgaact tagggataat taacctgttt
    40261 ccgttgcccg tacttgacgg ggggcatctg ctgttccttg cgatcgaaaa gatcaagggc
    40321 ggaccggtat ccgagcgggt tcaagacttt tgttatcgca ttggctcgat tctgctggtg
    40381 ctgttaatgg ggcttgcact tttcaatgat ttctctcggt tatgagagtt agttaggaag
    40441 aacgcataat aacgatggcg atgaaaaagt tgctcatagc gtcgctgctg tttagcagcg
    40501 ccaccgtata cggtgctgaa gggttcgtag tgaaagatat tcatttcgaa ggccttcagc
    40561 gtgtcgccgt tggtgcggcc ctcctcagta tgccggtgcg cacaggcgac acggttaatg
    40621 atgaagatat cagtaatacc attcgcgctc tgtttgctac cggcaacttt gaggatgttc
    40681 gcgtccttcg tgatggtgat acccttctgg ttcaggtaaa agaacgtccg accattgcca
    40741 gcattacttt ctccggtaac aaatcggtga aagatgacat gctgaagcaa aacctcgagg
    40801 cttctggtgt gcgtgtgggc gaatccctcg atcgcaccac cattgccgat atcgagaaag
    40861 gtctggaaga cttctactac agcgtcggta aatatagcgc cagcgtaaaa gctgtcgtga
    40921 ccccgctgcc gcgcaaccgt gttgacctaa aactggtgtt ccaggaaggt gtgtcagctg
    40981 aaatccagca aattaacatt gttggtaacc atgctttcac caccgacgaa ctgatctctc
    41041 atttccaact gcgtgacgaa gtgccgtggt ggaacgtggt aggcgatcgt aaataccaga
    41101 aacagaaact ggcgggcgac cttgaaaccc tgcgcagcta ctatctggat cgcggttatg
    41161 cccgtttcaa catcgactct acccaggtca gtctgacgcc agataaaaaa ggtatttacg
    41221 tcacggtgaa cattaccgaa ggcgatcagt acaagctttc tggcgttgaa gtgagcggca
    41281 accttgccgg gcactccgct gaaattgagc agctgactaa gatcgagccg ggcgagcagt
    41341 ataacggcac caaagtgacc aagatggaag atgacatcaa aaagcttctc ggtcgctatg
    41401 gttatgccta tccgcgcgta cagtcgatgc ctgaaattaa cgatgccgac aaaaccgtta
    41461 aattacgcgt gaacgttgat gcgggtaacc gtttctacgt gcgtaagatc cgttttgaag
    41521 gtaacgatac ctcgaaagat gccgtcctgc gtcgcgaaat gcgtcagatg gaaggtgcat
    41581 ggctgggaag cgatctggtc gatcagggta aggagcgtct gaatcgtctg ggcttctttg
    41641 aaactgtcga taccgatacc caacgtgttc cgggtagccc ggaccaggta gatgtcgtct
    41701 acaaggtaaa agagcgcaac accggtagct tcaacttcgg gattggttac ggtactgaaa
    41761 gtggcgtgag cttccaggct ggtgtgcagc aggataactg gttaggtaca ggttatgctg
    41821 ttggtatcaa cgggaccaaa aacgattacc agacctatgc tgaactgtcg gtaaccaacc
    41881 cgtacttcac tgtagatggt gtaagcctcg gtggtcgtct cttctataat gacttccagg
    41941 cagatgacgc cgacctgtcc gactatacca acaagagtta tggtacagac gtgacgttgg
    42001 gcttcccgat caacgaatat aactcgctgc gtgcaggtct gggttatgta cataactccc
    42061 tgtccaacat gcagcctcag gttgcgatgt ggcgttatct gtactctatg ggtgaacatc
    42121 cgagcacctc tgatcaggat aatagcttca aaacggacga cttcacgttc aactatggtt
    42181 ggacctataa caagcttgac cgtggttact tcccgacaga tggttcacgt gtcaacctga
    42241 ccggtaaagt gaccattcct ggatcggata acgaatacta caaagtgacg ttagacacgg
    42301 cgacttatgt gccgatcgat gacgatcaca aatgggttgt tctggggcgt acccgctggg
    42361 gttatggtga tggtttaggc ggcaaagaga tgccgttcta cgagaacttc tatgccggtg
    42421 gttccagcac cgtgcgtggc ttccagtcca atacaattgg tccgaaagca gtttacttcc
    42481 cgcatcaggc cagtaattat gatccggact atgattacga atgtgcgact caggacggcg
    42541 cgaaagacct gtgtaaatcg gatgatgctg taggcggtaa cgccatggcg gttgccagcc
    42601 tcgagttcat caccccgacg ccgtttatta gcgataagta tgctaactcg gttcgtactt
    42661 ccttcttctg ggatatgggt accgtttggg atacaaactg ggattccagc caatattctg
    42721 gatatccgga ctatagtgat ccaagcaata tccgtatgtc tgcgggtatc gcattacaat
    42781 ggatgtcccc attggggccg ttggtgttct cctacgccca gccgttcaaa aagtacgatg
    42841 gagacaaggc agaacagttc cagtttaaca tcggtaaaac ctggtaagtg ttctccacaa
    42901 aggaatgtag tggtagtgta gcgatgactt taggcgatca atataagatc gccgggccac
    42961 gcaaagaact gcaccctccg gtgcaaatgg gatggtaagg agtttattgt gaaaaagtgg
    43021 ttattagcgg caggtctcgg tttagcactg gcaacttctg ctcaggcggc tgacaaaatt
    43081 gcaatcgtca acatgggcag cctgttccag caggtagcgc agaaaaccgg tgtttctaac
    43141 acgctggaaa atgagttcaa aggccgtgcc agcgaactgc agcgtatgga aaccgatctg
    43201 caggctaaaa tgaaaaagct gcagtccatg aaagcgggca gcgatcgcac taagctggaa
    43261 aaagacgtga tggctcagcg ccagactttt gctcagaaag cgcaggcttt tgagcaggat
    43321 cgcgcacgtc gttccaacga agaacgcggc aaactggtta ctcgtatcca gactgctgtg
    43381 aaatccgttg ccaacagcca ggatatcgat ctggttgttg atgcaaacgc cgttgcttac
    43441 aacagcagcg atgtaaaaga catcactgcc gacgtactga aacaggttaa ataagtaatg
    43501 ccttcaattc gactggctga tttagcgcag cagttggatg cagaactaca cggtgatggc
    43561 gatatcgtca tcaccggcgt tgcgtccatg caatctgcac aaacaggtca cattacgttc
    43621 atggttaacc caaaataccg tgagcattta ggcttgtgcc aggcgtccgc ggttgtcatg
    43681 acccaggacg atcttccttt cgcgaaaagt gccgcgctgg tagtgaagaa tccctacctg
    43741 acttacgcgc gcatggcgca aattttagat accacgccgc agcccgcgca gaacattgca
    43801 cccagtgcgg tgatcgacgc gacggcgaag ctgggtaaca acgtatcgat tggcgctaac
    43861 gcggtgattg agtccggcgt tgaactgggc gataacgtga ttatcggtgc cggttgcttc
    43921 gtaggtaaaa acagcaaaat cggtgcaggt tcgcgtctct gggcgaacgt aaccatttac
    43981 catgagatcc agatcggtca gaattgcctg atccagtccg gaacagtggt aggcgcagac
    44041 ggctttggtt atgccaacga tcgtggtaac tgggtgaaga tcccacagat tggtcgcgta
    44101 attattggcg atcgcgtgga gatcggtgcc tgcacaacca tcgatcgcgg cgcgctggat
    44161 gacactatta ttggcaatgg cgtgatcatt gataaccagt gccagattgc acataacgtc
    44221 gtgattggcg acaatacggc ggttgccggt ggcgtcatta tggcgggcag cctgaaaatt
    44281 ggtcgttact gcatgatcgg cggagccagc gtaatcaacg ggcatatgga aatatgcgac
    44341 aaagtgacgg ttacgggcat gggtatggtg atgcgtccca tcactgaacc aggcgtctat
    44401 tcctcaggca ttccgctgca acccaacaaa gtctggcgca aaaccgctgc actggtgatg
    44461 aacattgatg acatgagcaa gcgtctgaaa tcgcttgagc gcaaggttaa tcaacaagac
    44521 taacgttcca tcttttgttc gccaaacttt acggcctgtc tcattcttac gattgcggca
    44581 ggccgtgtta ttattgtcgt ttcttatatt ttgacaggaa gagtatcttg actactaaca
    44641 ctcatactct gcagattgaa gagattttag aacttctgcc gcaccgtttc ccgttcttac
    44701 tggtggatcg cgtgctggat tttgaagaag gtcgttttct gcgcgcagta aaaaatgtct
    44761 ctgtcaatga gccattcttc cagggccatt tccctggaaa accgattttc ccgggtgtgc
    44821 tgattctgga agcaatggca caggcaacag gtattctggc gtttaaaagc gtaggaaaac
    44881 tggaaccggg tgagctgtac tacttcgctg gtattgacga agcgcgcttc aagcgcccgg
    44941 tcgtgcctgg cgatcaaatg atcatggaag tcactttcga aaaaacgcgc cgcggcctga
    45001 cccgttttaa aggggttgct ctggtcgatg gtaaagtagt ttgcgaagca acgatgatgt
    45061 gtgctcgtag ccgggaggcc tgatacgtga ttgataaatc cgcctttgtg catccaaccg
    45121 ccattgtgga agagggcgcg tcaattggcg cgaacgcaca cattggtcct ttttgtatcg
    45181 ttggacccca tgtcgaaatt ggtgagggta ccgtactgaa atctcacgtt gtcgtgaatg
    45241 gtcatactaa aattggccgc gataatgaga tttatcagtt cgcctccatc ggcgaagtta
    45301 accaggatct gaaatatgct ggcgaaccga cccgtgtgga aatcggcgat cgtaaccgca
    45361 ttcgcgaaag cgtcaccatt catcgtggca cagtccaggg cggtggattg acgaaggtgg
    45421 gcagcgacaa cttactgatg atcaacgcgc acattgcgca cgattgtacg gtaggtaacc
    45481 gctgtattct cgccaacaac gcaacgctgg cgggtcacgt atcggttgac gacttcgcga
    45541 tcatcggcgg catgaccgca gtccatcagt tctgcatcat tggtgcgcac gtgatggttg
    45601 gcggctgctc cggtgtggcg caggacgtcc ctccttatgt cattgcgcag ggtaaccacg
    45661 caacgccgtt cggtgtcaat atcgaagggc tgaagcgccg cggattcagc cgtgaggcga
    45721 ttaccgctat ccgcaatgcg tataagctga tttatcgtag cggtaaaacg ctcgatgaag
    45781 tgaaaccgga aattgctgaa ctggcggaaa catatccgga agtgaaagcc tttaccgatt
    45841 tctttgcacg ctcaacgcgc ggtctgattc gttaatgact gaacagcgtc cattaacgat
    45901 tgccctggtc gccggagaaa cctccggcga tatcctgggg gccggtttaa tccgcgctct
    45961 gaaagaacgt gtgcccaacg cccgctttgt tggtgttgcc gggccacgaa tgcaggctga
    46021 aggctgcgaa gcctggtacg aaatggaaga actggcggtg atgggcattg ttgaagtgct
    46081 cggtcgtctg cgtcgcttac tgcatattcg tgccgatctg acaaagcgtt ttggcgaact
    46141 gaagccagat atttttgttg gtattgatgc gcctgacttc aatattactc ttgaaggtaa
    46201 cctcaaaaag cagggtatca aaaccattca ttacgtcagt ccatccgtct gggcgtggcg
    46261 acagaaacgc gttttcaaaa taggcagagc caccgatctg gtgctcgcat ttctgccttt
    46321 cgaaaaagcg ttttatgaca aatacaatgt accgtgccgc tttatcggtc ataccatggc
    46381 tgatgccatg ccgttagatc cagataaaaa tgccgcccgt gatgtgctgg ggatccctca
    46441 cgatgcccac tgcctggcgt tgctaccagg gagccgtggt gcagaagttg aaatgcttag
    46501 tgccgatttc ctgaaaacgg cccagctttt gcgccagaca tatccggatc tcgaaatcgt
    46561 ggtgccactg gtgaatgcca aacgccgcga gcagtttgaa cgcatcaaag ctgaagtcgc
    46621 gccagacctt tcagttcatt tgctggatgg gatgggccgt gaggcgatgg tcgccagcga
    46681 tgcggcgcta ctggcgtcgg gtacggcagc cctggagtgt atgctggcga aatgcccgat
    46741 ggtggtggga tatcgcatga agccttttac cttctggttg gcgaagcggc tggtgaaaac
    46801 tgattatgtc tcgctgccaa atctgctggc gggtagagag ttagtcaaag aattattgca
    46861 ggaagagtgt gagccgcaaa aactggctgc ggcgctgtta ccgctgttgg cgaacgggaa
    46921 aaccagccac gcgatgcacg ataccttccg tgaactgcat cagcagatcc gctgcaatgc
    46981 cgatgagcag gcggcacaag ccgttctgga gttagcacaa tgatcgaatt tgtttatccg
    47041 cacacgcagc tggttgcggg tgtggatgaa gtcggacgcg ggccgttagt tggcgcggtc
    47101 gtcaccgctg cggtgatcct tgacccggcg cgcccgattg ccgggctgaa tgattccaaa
    47161 aagctgagcg aaaaacgccg tctggcgctc tatgaagaga tcaaagagaa agcgttgagc
    47221 tggagtctgg gccgcgcgga accccacgaa atcgacgagc tgaacattct tcatgcgacc
    47281 atgctggcga tgcagcgtgc cgtcgctggg ctgcatattg cgccggaata tgtgttgatt
    47341 gatggtaacc gctgcccgaa attaccgatg cctgcgatgg ctgtggtgaa aggcgatagc
    47401 cgcgtaccgg aaatcagtgc cgcgtctatc ctggcgaaag tgacgcgtga cgccgaaatg
    47461 gcggcgctgg atattgtttt cccgcaatat ggttttgccc aacacaaagg gtacccaacc
    47521 gcttttcatc tggaaaaact ggctgaacac ggcgcgaccg aacaccatcg gcgcagcttt
    47581 gggcctgtca aacgcgcact gggacttgcg tcctgattct tgtgtcgaga ttaagtaaac
    47641 cggaatctga agatgtctga accacgtttc gtacacctgc gggtgcacag cgactactcg
    47701 atgatcgatg gcctggccaa aaccgcaccg ttggtaaaaa aggcggcggc gttgggtatg
    47761 ccagcactgg cgatcaccga tttcaccaac ctttgtggtc tggtgaagtt ctacggagcg
    47821 ggacatggcg cagggattaa gcctatcgtc ggggcagatt ttaacgtcca gtgcgacctg
    47881 ctgggtgatg agttaaccca cctgacggta ctggcggcga acaataccgg ctatcagaat
    47941 ctgacgttgc tgatctcaaa agcgtatcag cgcgggtacg gtgccgccgg gccgatcatc
    48001 gatcgcgact ggcttatcga attaaacgaa gggttgatcc ttctttccgg cggacgcatg
    48061 ggcgacgtcg gacgcagtct tttgcgtggt aacagagcgc tggtagatga gtgtgtcgcg
    48121 ttttatgaag aacacttccc ggatcgctat tttctcgagc tgatccgcac cggcaggccg
    48181 gatgaagaaa gctatctgca cgcggcggtg gaactggcgg aagcgcgcgg tttgcccgtc
    48241 gtggcgacca acgacgtgcg ctttatcgac agcagcgact ttgacgcaca cgaaatccgc
    48301 gtcgcgatcc acgacggctt taccctcgac gatcctaaac gcccgcgtaa ctattcgccg
    48361 cagcaatata tgcgtagcga agaggagatg tgtgagctgt ttgccgacat ccccgaagcc
    48421 cttgccaaca ccgttgagat cgccaaacgc tgtaacgtaa ccgtgcgtct tggtgaatac
    48481 ttcctgccgc agttcccgac cggggacatg agcaccgaag attatctggt caagcgtgca
    48541 aaagagggcc tggaagagcg tctggccttt ttattccctg acgaggaaga acgtgttaag
    48601 cgccgcccgg aatatgacga acgtctggag actgaacttc aggttatcaa ccagatgggc
    48661 ttcccgggct acttcctcat cgttatggaa tttatccagt ggtcgaaaga taacggcgta
    48721 ccggtagggc caggccgtgg ctccggtgcg ggttcactgg tggcctacgc gctgaaaatc
    48781 accgacctcg atccgctgga atttgacctg ctgttcgaac gtttccttaa cccggaacgt
    48841 gtctccatgc ctgacttcga cgttgacttc tgtatggaga aacgcgatca ggttatcgag
    48901 cacgtagcgg acatgtacgg tcgtgatgcg gtatcgcaga tcatcacctt cggtacaatg
    48961 gcggcgaaag cggtgatccg cgacgtaggc cgcgtgctgg ggcatccgta cggctttgtc
    49021 gatcgtatct cgaaactgat cccgcccgat ccggggatga cgctggcgaa agcgtttgaa
    49081 gccgagccgc agctgccgga aatctacgaa gcggatgaag aagttaaggc gctgatcgac
    49141 atggcgcgca aactggaagg ggtcacccgt aacgccggta agcacgccgg tggggtggtt
    49201 atcgcgccga ccaaaattac cgattttgcg ccgctttact gcgatgaaga gggcaaacat
    49261 ccggtcaccc agtttgataa aagcgacgtt gaatacgccg gactggtgaa gttcgacttc
    49321 cttggtttgc gtacgctcac catcatcaac tgggcgctgg agatgatcaa caagcggcgg
    49381 gcgaagaatg gcgagccgcc gctggatatc gctgcgatcc cgctggatga taagaaaagc
    49441 ttcgacatgc tgcaacgctc ggaaaccacg gcggtattcc agcttgaatc gcgcggcatg
    49501 aaggacctga tcaagcgtct acaacctgac tgcttcgaag atatgatcgc cctagtggca
    49561 ctgttccgcc ccggtccgtt gcaatcaggg atggtggata actttatcga ccgtaaacat
    49621 ggtcgtgaag agatctccta tccggacgta cagtggcagc atgaaagcct gaaaccggta
    49681 ctggagccaa cctacggcat tatcctgtat caggaacagg tcatgcagat tgcgcaggtg
    49741 ctttctggtt ataccctcgg tggcgcggat atgctgcgtc gtgcgatggg taagaaaaag
    49801 ccggaagaga tggctaagca acgttctgta tttgctgaag gtgcagaaaa gaacggaatc
    49861 aacgctgaac tggcgatgaa aatcttcgac ctggtggaga aattcgctgg ttacggattt
    49921 aacaaatcgc actctgcggc ctatgctttg gtgtcatatc aaacgttatg gctgaaagca
    49981 cactatcctg cggagtttat ggcggcggta atgaccgccg atatggacaa caccgagaag
    50041 gtggtgggtc tggtggatga gtgctggcgg atggggctga aaatcctgcc accagatata
    50101 aactccggtc tttaccattt ccacgtcaac gacgacggcg aaatcgtgta tggtattggc
    50161 gcgatcaaag gggtcggtga aggtccgatt gaggccatca tcgaagcccg taataaaggc
    50221 ggctacttcc gcgaactgtt tgatctctgc gcccgtaccg acaccaaaaa gttgaaccgt
    50281 cgcgtgctgg aaaaactgat catgtccggg gcgtttgacc gtcttgggcc acaccgcgcg
    50341 gcgctgatga actcgctggg tgatgcgtta aaagcggcag atcaacacgc gaaagcggaa
    50401 gctatcggtc aggccgatat gttcggcgtg ctggccgaag agccggaaca aattgaacaa
    50461 tcctacgcca gctgccaacc gtggccggag caggtggtat tagatgggga acgtgaaacg
    50521 ttaggcctgt acctgaccgg acaccctatc aaccagtatt taaaagagat tgagcgttat
    50581 gtcggaggcg taaggctgaa agacatgcac ccgacagaac gtggtaaagt catcacggct
    50641 gcggggctcg ttgttgccgc gcgggttatg gtcaccaagc gcggcaatcg tatcggtatc
    50701 tgcacgctgg atgaccgttc cgggcggctg gaagtgatgt tgtttactga cgccctggat
    50761 aaataccagc aattgctgga aaaagaccgc atacttatcg tcagcggaca ggtcagcttt
    50821 gatgacttca gcggtgggct taaaatgacc gctcgcgaag tgatggatat tgacgaagcc
    50881 cgggaaaaat atgctcgcgg gcttgctatc tcgctgacgg acaggcaaat tgatgaccag
    50941 cttttaaacc gactccgtca gtctctggaa ccccaccgct ctgggacaat tccagtacat
    51001 ctctactatc agagggcgga tgcacgcgcg cggttgcgtt ttggcgcgac gtggcgtgtc
    51061 tctccgagcg atcgtttatt aaacgatctc cgtggcctca ttggttcgga gcaggtggaa
    51121 ctggagtttg actaatacag gaatactatg agtctgaatt tccttgattt tgaacagccg
    51181 attgcagagc tggaagcgaa aatcgattct ctgactgcgg ttagccgtca ggatgagaaa
    51241 ctggatatta acatcgatga agaagtgcat cgtctgcgtg aaaaaagcgt agaactgacg
    51301 cgtaaaatct tcgccgatct tggtgcatgg cagattgcgc aactggcacg ccatccacag
    51361 cgtccttata ccctggatta cgttcgcctg gcatttgatg aatttgacga actggctggc
    51421 gaccgcgcgt atgcagacga taaagctatc gtcggtggta tcgcccgtct tgacggtcgt
    51481 ccggtgatga tcattggtca tcaaaaaggt cgtgaaacca aagaaaaaat tcgccgtaac
    51541 tttggtatgc cagcgccaga aggttaccgc aaagcactgc gtctgatgca aatggctgaa
    51601 cgctttaaga tgcctatcat cacctttatc gacaccccgg gggcttatcc gggcgtgggc
    51661 gcagaagaac gtggtcagtc tgaagccatt gcacgcaacc tgcgtgaaat gtctcgcctc
    51721 ggtgtaccgg tagtttgtac ggttatcggt gaaggtggtt ctggcggcgc gctggcgatt
    51781 ggcgtgggcg ataaagtgaa tatgctgcaa tacagcacct attccgttat ctcgccggaa
    51841 ggttgtgcgt ccattctgtg gaagagcgct gacaaagcgc cgctggcagc tgaagcgatg
    51901 ggtatcatcg ctccgcgtct gaaagaacta aaactgatcg actccatcat cccggaaccg
    51961 ctgggtggtg ctcaccgtaa cccggaagcg atggcggcat cgttgaaagc gcaactgctg
    52021 gcggatctgg ccgatctcga cgtgttaagc actgaagatt taaaaaatcg tcgttatcag
    52081 cgcctgatga gctacggtta cgcgtaattc gcaaaagttc tgaaaaaggg tcacttcggt
    52141 ggcccttttt tatcgccacg gtttgagcag gctatgatta aggaaggatt ttccaggagg
    52201 aacacatgaa catcattgcc attatgggac cgcatggcgt cttttataaa gatgagccca
    52261 tcaaagaact ggagtcggcg ctggtggcgc aaggctttca gattatctgg ccacaaaaca
    52321 gcgttgattt gctgaaattt atcgaacata accctcgaat ttgcggcgtg atttttgact
    52381 gggatgagta cagtctcgat ttatgcagcg atatcaatca acttaatgaa tatctcccgc
    52441 tttatgcctt catcaacacc cactcgacga tggatgtcag cgtgcaggat atgcggatgg
    52501 cgctctggtt ttttgaatat gcgctggggc aggcggaaga tatcgccatt cgtatgcgtc
    52561 agtacaccga cgaatatctt gataacatta caccgccgtt cacgaaagcc ttgtttacct
    52621 acgtcaaaga gcggaagtac accttttgta cgccggggca tatgggcggc accgcatatc
    52681 aaaaaagccc ggttggctgc ctgttttatg attttttcgg cgggaatacc ctcaaggctg
    52741 atgtctctat ttcggtcacc gagcttggtt cgttgctcga ccacaccggg ccacacctgg
    52801 aagcggaaga gtacatcgcg cggacttttg gcgcggaaca gagttatatc gttaccaacg
    52861 gaacatcgac gtcgaacaaa attgtgggta tgtacgccgc gccatccggc agtacgctgt
    52921 tgatcgaccg caattgtcat aaatcgctgg cgcatctgtt gatgatgaac gatgtagtgc
    52981 cagtctggct gaaaccgacg cgtaatgcgt tggggattct tggtgggatc ccgcgccgtg
    53041 aatttactcg cgacagcatc gaagagaaag tcgctgccac cacgcaggca caatggccgg
    53101 ttcatgcggt gattaccaac tccacctatg atggcttgct ctacaacacc gactggatca
    53161 aacagacgct ggatgtcccg tcgatccact tcgattccgc ctgggtgccg tacacccatt
    53221 ttcatccgat ctaccagggg aaaagtggta tgagcggcga gcgtgttgcg ggaaaagtga
    53281 tcttcgaaac gcaatcgacc cacaaaatgc tggcggcgtt atcgcaggct tcgctgatcc
    53341 acattaaagg cgagtatgac gaagaggcgt ttaacgaagc ctttatgatg cataccacca
    53401 cctcgcccag ttatcccatt gttgcttcgg ttgagacggc ggcggcgatg ctgcgtggta
    53461 atccgggcaa acggctgatt aaccgttcgg tggagcgggc gctgcatttt cgcaaagagg
    53521 tccagcggtt gcgggaagag tctgacggtt ggtttttcga tatctggcaa ccgccgcagg
    53581 tggatgaagc cgaatgctgg cccgttgcgc taggcgaaca gtggcacggc tttaacgatg
    53641 cggatgccga tcatatgttt ctcgatccgg ttaaagtcac tattttgaca ccggggatgg
    53701 acgaacaggg caatatgagc gaggagggga tcccggcggc gctggtggca aaattcctcg
    53761 acgaacgtgg gatcgtagta gagaaaaccg gcccttataa cctgctgttt ctctttagta
    53821 ttggtatcga taaaaccaaa gcaatgggat tattgcgtgg gttgacggaa tttaaacgct
    53881 cttacgatct caacctgcgg atcaaaaata tgctacccga tctctatgct gaagatcccg
    53941 atttctaccg caatatgcgt attcaggatc tggcgcaagg gatccataag ctgattcgta
    54001 aacacgatct tcccggtttg atgttgcggg cattcgatac tttgccggaa atgatcatga
    54061 cgccacatca ggcatggcag agacagatta aaggtgaagt agaaaccatt gcgctggaac
    54121 aactggtcgg tagagtatcg gcaaatatga tcctgcctta tccaccgggc gtaccgctgt
    54181 tgatgccagg agaaatgctg accaaagaga gccgcacggt actcgatttt ctactgatgc
    54241 tttgttccgt agggcaacat taccccggtt ttgaaacgga tattcacggc gcgaaacagg
    54301 acgaagacgg cgtttaccgc gtacgagtcc taaaaatggc gggataactt gccagagcgg
    54361 cttccgggcg agtaacgtgc tgttaacaaa taaaggagac gttatgctgg gtttaaaaca
    54421 ggttcaccat attgcgatta ttgcgacgga ttatgcggtg agcaaagctt tctactgcga
    54481 tattcttggt ttcacgctgc aaagcgaagt ctatcgcgaa gcgcgcgact cgtggaaagg
    54541 ggatttggcg cttaatgggc aatatgtgat tgagcttttc tcttttccgt tcccgccgga
    54601 gcgccccagc cgaccggaag cttgcggcct gcgtcatctg gcttttagcg ttgatgacat
    54661 cgatgcggca gtggcgcacc ttgaaagcca taacgtgaag tgtgaagcca tccgtgtcga
    54721 tccatacacg caaaaacgct tcaccttctt taacgatccg gacgggctgc cgttggaact
    54781 gtatgagcag taaggcttgt catcgccgca tttgcccggt aacgtgccgg gcattgctac
    54841 tgtaaaatcg caccatcatg acactcacgc tcaatagaca acttctcacc tcacgccaga
    54901 ttctggtggc ctttagcggc gggcttgact ccaccgttct gctgcatcag ttggtgcagt
    54961 ggcggacgga aaatccgggc gtcactctgc gcgctattca tgtgcatcac ggtttaagtg
    55021 ccaatgccga tgcctgggtt acgcattgcg aaaacgtctg ccaacagtgg caggtgccgc
    55081 tggtggtcga acgcgtgcaa cttgcgcaag aaggactggg cattgaggcc caggcgcggc
    55141 aggcgcgtta tcaggcattt gcccgcacct tgttgcccgg tgaagtgctg gtcaccgcgc
    55201 aacatctcga cgatcaatgt gaaacttttc tgctggcgct aaaacgcggt agcggccctg
    55261 ccgggctttc ggctatggcg gaagtttcgg agtttgccgg aacacggctt atccgcccgt
    55321 tgctcgcccg cacgcgggga gaactggagc agtgggcatt ggcgcatggt ttacgctgga
    55381 ttgaagacga aagtaatcag gacgactcat acgatcgtaa ctttctgcgc ctgcgcgtag
    55441 tgccgttatt gcagcagcgt tggccgcatt ttgcggaagc aacggcccgc agcgccgcac
    55501 tttgtgctga acaagagagt ctgttggatg aactgctggc ggatgattta gcacactgcc
    55561 aaacgtcgca ggggacgctg cagattgcgc caatgctggc gatgagtgat gcccgccgcg
    55621 cggcgattat ccgtcgctgg ctggcagggc agaatgcacc aatgccttcc cgcgacgcgc
    55681 tggtgcggat ctggcaggaa gtggcgctgg cgcgggaaga tgcctccccc tgtttacgct
    55741 tgggcgcgtt tgaaatccgc cgctatcagt cgcaactgtg gtggattaaa tccgtcaccg
    55801 ggcaaagtga aaccattgtg ccgtggcaga cgtggctaca accgctggaa ttaccggcgg
    55861 ggctgggaag cgtacagctt actgcgggag gcgatattcg ccctccgcgt gcagacgaag
    55921 cggtcagcgt gcgtttcaaa gcggcaggat tgctgcatat tgtcgggcgc aacggcggac
    55981 gtaagctgaa gaaaatctgg caagagttag gcgtgccgcc gtggctacgt gacaccacgc
    56041 cactgctgtt ttatggcgaa acgctgattg cggcggcagg ggtatttgtg acgcaagaag
    56101 gtgtggctga aggtgagaat ggcgtaagtt ttgtctggca gaaaacgctt agttaagtga
    56161 aagccggata agacgcatca aacgtcgcat ccggcgaaag tcaatcagga ctcgctcacc
    56221 accaccgtac cgatttccgg gtggctaaag ctggtaattt tatccagacg tagctcacgg
    56281 gtttcgccag cggcctcgac gaccagatac tccacatttt tgcgggagac taaatcgctg
    56341 gcttttgcct gcaatttttc gccatctttc agctcaagtg tcagcattaa atgatgctgg
    56401 caggcgagct caagattatc gtaatcatca caattgattg gttgatacgt atcattcatt
    56461 gacataatcg ctcaccagta agtttgccgc agcgtatgct gctttttccc tgacagcctc
    56521 agaaagggcg tcgtcggcag ccatttcatt cagcactttc aaaacgcagc ccagcgcgtc
    56581 cggaacgtat cctaagtctc cgctggcgat ttccgcgtac cgcttgcgta ttaactcaca
    56641 atatttttcc acatgccctc ctgtcagcac tctgacttaa ccgtggatgc aagtctaagc
    56701 ctacgaagat aaactctgtt tcgcaaggtg actataccac actcatgtct gcaatatcag
    56761 cgccgcaact gcacgtattc cgttacaatg gcctcctgat tcgaaaggag ttttcttatg
    56821 gcgcttaaag cgacaattta taaagcgacg gttaatgtgg ccgatctcga ccgcaaccag
    56881 tttctcgatg cctctctgac gctggcgcgc cacccttcag aaacccagga gcgtatgatg
    56941 ctgcgcttgc tggcgtggct gaaatatgct gatgaacgtc tgcaatttac ccgtggtttg
    57001 tgtgccgatg atgagccgga agcgtggctg cgtaacgatc acctgggcat tgatttgtgg
    57061 attgagctgg ggctgccgga tgagcggcgg attaagaaag cctgcaccca ggccgcagaa
    57121 gtggcgctgt ttacctataa tagtcgggcg gcgcaaatct ggtggcagca aaatcagagc
    57181 aaatgtgtgc agtttgccaa tctttccgtc tggtatctgg acgatgaaca actggcgaaa
    57241 gtaagcgcct ttgccgatcg taccatgacg ctgcaggcaa cgattcagga tggcgtgatc
    57301 tggttatcgg acgataagaa taatctggaa gttaacttaa ccgcctggca acaaccttca
    57361 tgattgtgat ttcccgacat gttgctattc ccgatggtga gcttgagatc accgccattc
    57421 gtgcgcaggg cgcgggcggg cagcatgtta ataagacctc aacggccatt catctgcgtt
    57481 ttgacattcg ggcgtccagc ctgccagagt attacaaaga gcgtctactc gccgccagcc
    57541 atcatttgat cagcagtgat ggcgtgattg tcattaaggc acaggaatac cgcagtcagg
    57601 aactgaaccg cgaagccgcg ctagcccggc tggtggcagt gattaaagat ttaacaacag
    57661 aacaaaaagc ccgacgaccc acgcggccca cccgtgcatc gaaagagcgc aggctggcat
    57721 cgaaagcaca aaaatcaagc gtgaaggcga tgcgcggcaa agtacgcagc ggtcgggaat
    57781 aaaaagaagg aatagatggt gaaaaaagcg atagtgacag cgatggctgt aatcagcctc
    57841 tttactctga tgggatgtaa taaccgggcc gaagtcgata cgctttctcc agcacaggct
    57901 gccgaactga aaccgatgcc gcaaagttgg cgcggcgtgc tgccgtgtgc tgattgcgaa
    57961 ggaatcgaaa cctctctgtt cctcgaaaaa gacggaacat gggtgatgaa tgagcgttat
    58021 ctcggtgctc gtgaagaacc ttcctccttc gcttcctacg gtacatgggc gcgaaccgct
    58081 gacaagctgg tattaaccga cagcaaaggt gaaaagtcat attatcgggc gaaaggcgat
    58141 gcgctggaga tgctcgatcg tgaaggcaat ccgattgaat cgcagttcaa ctatacgctg
    58201 gaaccggcac aatctagttt acctatgacg ccgatgaccc tgcgaggcat gtatttttat
    58261 atggctgatg cggcgacctt cactgattgc gcgaccggaa aacgtttcat ggtagcgaat
    58321 aacgcagagc tggagcgtag ctacctggct gcgcgcggtc acagtgaaaa accggtgtta
    58381 ctgtcagtag aaggtcactt tacgcttgag gctaatccgg atactggtgc gccaaccaaa
    58441 gtattagcgc ccgatacggc gggtaaattt tacccgaata aggattgcag tagtttgggg
    58501 cagtaaacct gcttaaggca gaaacaaacg caa
//
LOCUS       DAZKHF020000003        53226 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2201S31241
            SAMN43267800-rid25106413.denovo.003, whole genome shotgun sequence.
ACCESSION   DAZKHF020000003 DAZKHF020000000
VERSION     DAZKHF020000003.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267800
            Sequence Read Archive: SRR30307263
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 53226)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 53226)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 53226)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350581.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350581.2
            Genome Coverage       :: 151x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:21:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,652
            CDSs (total)                      :: 5,557
            Genes (coding)                    :: 5,320
            CDSs (with protein)               :: 5,320
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 237
            CDSs (without protein)            :: 237
            Pseudo Genes (ambiguous residues) :: 0 of 237
            Pseudo Genes (frameshifted)       :: 102 of 237
            Pseudo Genes (incomplete)         :: 130 of 237
            Pseudo Genes (internal stop)      :: 52 of 237
            Pseudo Genes (multiple problems)  :: 41 of 237
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..53226
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267800-rid25106413.denovo.003"
                     /serotype="O 26:H11"
                     /isolate="2201S31241"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-01"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            271..2664
                     /gene="malP"
                     /locus_tag="ACHOSC_RS00540"
                     /old_locus_tag="pgaptmp_000108"
     CDS             271..2664
                     /gene="malP"
                     /locus_tag="ACHOSC_RS00540"
                     /old_locus_tag="pgaptmp_000108"
                     /EC_number="2.4.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008184 - glycogen phosphorylase activity
                     [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="maltodextrin phosphorylase"
                     /protein_id="HGU4546124.1"
                     /translation="MSQPIFNDKQFQEALSRQWQRYGLNSAAEMTPRQWWLAVSEALA
                     EMLRAQPFAKPVANQRHVNYISMEFLIGRLTGNNLLNLGWYQDVQDSLKAYDINLTDL
                     LEEEIDPALGNGGLGRLAACFLDSMATVGQSATGYGLNYQYGLFRQSFVDGKQVEAPD
                     DWHRSNYPWFRHNEALDVQVGIGGKVTKDGRWEPEFTITGQAWDLPVVGYRNGVAQPL
                     RLWQATHAHPFDLTKFNDGDFLRAEQQGINAEKLTKVLYPNDNHTAGKKLRLMQQYFQ
                     CACSVADILRRHHLAGRKLHELADYEVIQLNDTHPTIAIPELLRVLIDEHQMSWDDAW
                     AITSKTFAYTNHTLMPEALERWDVKLVKGLLPRHMQIINEINTRFKTLVEKTWPGDEK
                     VWAKLAVVHDKQVHMANLCVVGGFAVNGVAALHSDLVVKDLFPEYHQLWPNKFHNVTN
                     GITPRRWIKQCNPALAALLDKSLQKEWANDLDQLINLEKFADDAKFRQQYREIKQANK
                     VRLAEFVKVRTGIEINPQAIFDIQIKRLHEYKRQHLNLLHILALYKEIRENPQADRVP
                     RVFLFGAKAAPGYYLAKNIIFAINKVADVINNDPLVGDKLKVVFLPDYCVSAAEKLIP
                     AADISEQISTAGKEASGTGNMKLALNGALTVGTLDGANVEIAEKVGEENIFIFGHTVE
                     QVKAILAKGYDPVKWRKKDKVLDAVLKELESGKYSDGDKHAFDQMLHSIGKQGGDPYL
                     VMADFAAYVEAQKQVDVLYRDQEAWTRAAILNTARCGMFSSDRSIRDYQARIWQAKR"
     gene            2674..4758
                     /gene="malQ"
                     /locus_tag="ACHOSC_RS00545"
                     /old_locus_tag="pgaptmp_000109"
     CDS             2674..4758
                     /gene="malQ"
                     /locus_tag="ACHOSC_RS00545"
                     /old_locus_tag="pgaptmp_000109"
                     /EC_number="2.4.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004134 - 4-alpha-glucanotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-alpha-glucanotransferase"
                     /protein_id="HGU4546125.1"
                     /translation="MESKRLDNAALAAGISPNYINAHGKPQSISAETKRRLLDAMHQR
                     TATKVAVTPVPNVMVYTSGKKMPMVVEGSGEYSWLLTTEEGTQYKGHVTGGKAFNLPT
                     KLPEGYHTLTLTQDDQRAHCRVIVAPKRCYEPQALLNKQKLWGACVQLYTLRSEKNWG
                     IGDFGDLKAMLVDVAKRGGSFIGLNPIHALYPANPESASPYSPSSRRWLNVIYIDVNA
                     VEDFHLSEEAQAWWQLPTTQQTLQQARDADWVDYSTVTALKMTALRMAWKGFAQRDDE
                     QMTAFRQFVAEQGDSLFWQAAFDALHAQQVKEDEMRWGWPAWPEMYQNVDSPEVRQFC
                     EEHRDDVDFYLWLQWLAYSQFAACWEISQGYEMPIGLYRDLAVGVAEGGAETWCDREL
                     YCLKASVGAPPDILGPLGQNWGLPPMDPHIITARAYEPFIELLRANMQNCGALRIDHV
                     MSMLRLWWIPYGETADQGAYVHYPVDDLLSILALESKRHRCMVIGEDLGTVPVEIVGK
                     LRSSGVYSYKVLYFENDHEKTFRAPKAYPEQSMAVAATHDLPTLRGYWESGDLTLGKT
                     LGLYPDEVVLRGLYQDRELAKQGLLDALHKYGCLPKRAGHKASLMSMTPTLNRGLQRY
                     IADSNSALLGLQPEDWLDMAEPVNIPGTSYQYKNWRRKLSATLESMFADDGVNKLLKD
                     LDRRRRAAAKKK"
     gene            complement(4803..6119)
                     /gene="gntT"
                     /locus_tag="ACHOSC_RS00550"
                     /old_locus_tag="pgaptmp_000110"
     CDS             complement(4803..6119)
                     /gene="gntT"
                     /locus_tag="ACHOSC_RS00550"
                     /old_locus_tag="pgaptmp_000110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gluconate transporter"
                     /protein_id="HGU4546126.1"
                     /translation="MPLVIVAIGVILLLLLMIRFKMNGFIALVLVALAVGLMQGMPLD
                     KVIGSIKAGVGGTLGSLALIMGFGAMLGKILADCGGAQRIATTLIAKFGKKHIQWAVV
                     LTGFTVGFALFYEVGFVLMLPLVFTIAASANIPLLYVGVPMAAALSVTHGFLPPHPGP
                     TAIATIFNADMGKTLLYGTILAIPTVILAGPVYARVLKGIDKPIPEGLYSAKTFSEEE
                     MPSFGVSVWTSLVPVVLMAMRAIAEMILPKGHAFLPVAEFLGDPVMATLIAVLIAMFT
                     FGLNRGRSMDQINDTLVSSIKIIAMMLLIIGGGGAFKQVLVDSGVDKYIASMMHETNI
                     SPLLMAWSIAAVLRIALGSATVAAITAGGIAAPLIATTGVSPELMVIAVGSGSVIFSH
                     VNDPGFWLFKEYFNLTIGETIKSWSMLETIISVCGLVGCLLLNMVI"
     gene            complement(6480..7055)
                     /gene="nfuA"
                     /locus_tag="ACHOSC_RS00555"
                     /old_locus_tag="pgaptmp_000111"
     CDS             complement(6480..7055)
                     /gene="nfuA"
                     /locus_tag="ACHOSC_RS00555"
                     /old_locus_tag="pgaptmp_000111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016942932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA];
                     GO_process: GO:0051604 - protein maturation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S biogenesis protein NfuA"
                     /protein_id="HGU4546127.1"
                     /translation="MIRISDAAQAHFAKLLANQEEGTQIRVFVINPGTPNAECGVSYC
                     PPDAVEATDTALKFDLLTAYVDELSAPYLEDAEIDFVTDQLGSQLTLKAPNAKMRKVA
                     DDAPLMERVEYMLQSQINPQLAGHGGRVSLMEITEDGYAILQFGGGCNGCSMVDVTLK
                     EGIEKQLLNEFPELKGVRDLTEHQRGEHSYY"
     gene            complement(7114..7797)
                     /gene="gntX"
                     /locus_tag="ACHOSC_RS00560"
                     /old_locus_tag="pgaptmp_000112"
     CDS             complement(7114..7797)
                     /gene="gntX"
                     /locus_tag="ACHOSC_RS00560"
                     /old_locus_tag="pgaptmp_000112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417872.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA utilization protein GntX"
                     /protein_id="HGU4546128.1"
                     /translation="MLTVPGLCWLCRMPLALGHWGICSVCSRATRTDKTLCPQCGLPA
                     THSHLPCGRCLQKPPPWQRLVTVADYAPPLSPLIHQLKFSRRSEIASALSRLLLLEVL
                     HARRTTGLQLPDRIISVPLWQRRHWRRGFNQSDLLCQPLSRWLHCQWDSEAVTRTRAT
                     ATQHFLSARLRKRNLKNAFRLELPVQGRHMVIVDDVVTTGSTVAEIAQLLLRNGAATV
                     QVWCLCRTL"
     gene            7835..8605
                     /gene="bioH"
                     /locus_tag="ACHOSC_RS00565"
                     /old_locus_tag="pgaptmp_000113"
     CDS             7835..8605
                     /gene="bioH"
                     /locus_tag="ACHOSC_RS00565"
                     /old_locus_tag="pgaptmp_000113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052689 - carboxylic ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pimeloyl-ACP methyl ester esterase BioH"
                     /protein_id="HGU4546129.1"
                     /translation="MNNIWWQTKGQGNVHLVLLHGWGLNAEVWRCIDEELSSHFTLHL
                     VDLPGFGRSRGFGALSLADMAEAVLQQAPDKAIWLGWSLGGLVASQIVLTHPERVQAL
                     VTVASSPCFSARDEWPGIKPDVLAGFQQQLSDDFQRTVERFLALQTMGTETARQDARA
                     LKKTVLALPMPEVDVLNGGLEILKTVDLRQPLQNVSMPFLRLYGYLDGLVPRKVVPML
                     DKLWPHSESYIFAKAAHAPFISHPVEFCHLLVALKQRV"
     gene            complement(8634..9512)
                     /gene="rpnA"
                     /locus_tag="ACHOSC_RS00570"
                     /old_locus_tag="pgaptmp_000114"
     CDS             complement(8634..9512)
                     /gene="rpnA"
                     /locus_tag="ACHOSC_RS00570"
                     /old_locus_tag="pgaptmp_000114"
                     /EC_number="3.1.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination-promoting nuclease RpnA"
                     /protein_id="HGU4546130.1"
                     /translation="MSKKQSSTPHDALFKLFLRQPDTARDFLAFHLPAPIHALCDMKT
                     LKLESSSFIDDDLRESYSDVLWSVKTEQGPGYIYCLIEHQSTSNKLIAFRMMRYAIAA
                     MQNHLDAGYKTLPMVVPLLFYHGIESPYPYSLCWLDCFADPKLARQLYASAFPLIDVT
                     VMPDDEIMQHRRMALLELIQKHIRQRDLMGLVEQMACLLSSGYANDRQIKGLFNYILQ
                     TGDAVRFNDFIDGVAERSPKHKESLMTIAERLRQEGEQSKALHIAKIMLESGVPLTDI
                     MRFTGVSEEELAAASQ"
     gene            complement(9715..9951)
                     /gene="feoC"
                     /locus_tag="ACHOSC_RS00575"
                     /old_locus_tag="pgaptmp_000115"
     CDS             complement(9715..9951)
                     /gene="feoC"
                     /locus_tag="ACHOSC_RS00575"
                     /old_locus_tag="pgaptmp_000115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="[Fe-S]-dependent transcriptional repressor FeoC"
                     /protein_id="HGU4546131.1"
                     /translation="MASLIQVRDLLALRGRMEAAQISQTLNTPQPMINAMLQQLESMG
                     KAVRIQEEPDGCLSGSCKSCPEGKACLREWWALR"
     gene            complement(9951..12272)
                     /gene="feoB"
                     /locus_tag="ACHOSC_RS00580"
                     /old_locus_tag="pgaptmp_000116"
     CDS             complement(9951..12272)
                     /gene="feoB"
                     /locus_tag="ACHOSC_RS00580"
                     /old_locus_tag="pgaptmp_000116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0015093 - ferrous iron transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006826 - iron ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe(2+) transporter permease subunit FeoB"
                     /protein_id="HGU4546132.1"
                     /translation="MKKLTIGLIGNPNSGKTTLFNQLTGARQRVGNWAGVTVERKEGQ
                     FSTTDHQVTLVDLPGTYSLTTISSQTSLDEQIACHYILSGDADLLINVVDASNLERNL
                     YLTLQLLELGIPCIVALNMLDIAEKQNIRIEIDALSARLGCPVIPLVSTRGRGIEALK
                     LAIDRYKANENVELVHYAQPLLNEADSLAKVMPSDIPLKQRRWLGLQMLEGDIYSRAY
                     AGEASQHLDAALARLRNEMDDPALHIADARYQCIAAICDVVSNTLTAEPSRFTTAVDK
                     IVLNRFLGLPIFLFVMYLMFLLAINIGGALQPLFDVGSVALFVHGIQWIGYTLHFPDW
                     LTIFLAQGLGGGINTVLPLVPQIGMMYLFLSFLEDSGYMARAAFVMDRLMQALGLPGK
                     SFVPLIVGFGCNVPSVMGARTLDAPRERLMTIMMAPFMSCGARLAIFAVFAAAFFGQN
                     GALAVFSLYMLGIVMAVLTGLMLKYTIMRGEATPFVMELPVYHVPHVKSLIIQTWQRL
                     KGFVLRAGKVIIIVSIFLSAFNSFSLSGKIVDNINDSALASVSRVITPVFKPIGVHED
                     NWQATVGLFTGAMAKEVVVGTLNTLYTAENIQDEEFNPAEFNLGEELFSAIDETWQSL
                     KDTFSLSVLMNPIEASKGDGEMGTGAMGVMDQKFGSAAAAYSYLIFVLLYVPCISVMG
                     AIARESSRGWMGFSILWGLNIAYSLATLFYQVASYSQHPTYSLVCILAVILFNIVVIG
                     LLRRARSRVDIELLATRKSVSSCCAASTTGDCH"
     gene            complement(12289..12516)
                     /gene="feoA"
                     /locus_tag="ACHOSC_RS00585"
                     /old_locus_tag="pgaptmp_000117"
     CDS             complement(12289..12516)
                     /gene="feoA"
                     /locus_tag="ACHOSC_RS00585"
                     /old_locus_tag="pgaptmp_000117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferrous iron transporter A"
                     /protein_id="HGU4546133.1"
                     /translation="MQYTPDTAWKITGFSREISPAYRQKLLSLGMLPGSSFNVVRVAP
                     LGDPIHIETRRVSLVLRKKDLALLEVEAVSC"
     gene            complement(12954..15275)
                     /gene="yhgF"
                     /locus_tag="ACHOSC_RS00590"
                     /old_locus_tag="pgaptmp_000118"
     CDS             complement(12954..15275)
                     /gene="yhgF"
                     /locus_tag="ACHOSC_RS00590"
                     /old_locus_tag="pgaptmp_000118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417866.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003729 - mRNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Tex family protein"
                     /protein_id="HGU4546134.1"
                     /translation="MMNDSFCRIIAGEIQARPEQVDAAVRLLDEGNTVPFIARYRKEI
                     TGGLDDTQLRNLETRLSYLRELEERRQAILKSISEQGKLTDDLAKAINATLSKTELED
                     LYLPYKPKRRTRGQIAIEAGLEPLADLLWSDPSHTPEVAAAQYVDADKGVADTKAALD
                     GARYILMERFAEDAALLAKVRDYLWKNAHLVSTVMSGKEEEGAKFRDYFDHHEPLSTV
                     PSHRALAMFRGRNEGVLQLSLNADPQFEEPPKESYCEQIIMDHLGLRLNNAPADSWRK
                     GVVSWTWRIKVLMHLETELMGTVRERAEDEAINVFARNLHDLLMAAPAGLRATMGLDP
                     GLRTGVKVAVVDATGKLVATDTIYPHTGQTAKAAMTVAALCEKHNVELVAIGNGTASR
                     ETERFYLDVQKQFPKVTAQKVIVSEAGASVYSASELAAQEFPDLDVSLRGAVSIARRL
                     QDPLAELVKIDPKSIGVGQYQHDVSQTQLARKLDAVVEDCVNAVGVDLNTASVPLLTR
                     VAGLTRMMAQNIVAWRDENGQFKNRQQLLKVSRLGPKAFEQCAGFLRINHGDNPLDAS
                     TVHPEAYPVVERILAATQQALKDLMGNSSELRNLKASDFTDEKFGVPTVTDIIKELEK
                     PGRDPRPEFKTAQFADGVETMNDLQPGMILEGAVTNVTNFGAFVDIGVHQDGLVHISS
                     LSNKFVEDPHTVVKAGDIVKVKVLEVDLQRKRIALTMRLDEQPGETNARRGGGNERPQ
                     NNRPAAKPRGREAQPAGNSAMMDALAAAMGKKR"
     gene            complement(15372..15848)
                     /gene="greB"
                     /locus_tag="ACHOSC_RS00595"
                     /old_locus_tag="pgaptmp_000119"
     CDS             complement(15372..15848)
                     /gene="greB"
                     /locus_tag="ACHOSC_RS00595"
                     /old_locus_tag="pgaptmp_000119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312275.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0032784 - regulation of DNA-templated
                     transcription elongation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription elongation factor GreB"
                     /protein_id="HGU4546135.1"
                     /translation="MKTPLVTREGYEKLKQELNYLWREERPEVTKKVTWAASLGDRSE
                     NADYQYNKKRLREIDRRVRYLTKCMENLKIVDYSPQQEGKVFFGAWVEIENDDGVTHR
                     FRIVGYDEIFGRKDYISIDSPMARALLKKEVGDLAVVNTPAGEASWYVNAIEYVKP"
     gene            16076..16795
                     /gene="ompR"
                     /locus_tag="ACHOSC_RS00600"
                     /old_locus_tag="pgaptmp_000120"
     CDS             16076..16795
                     /gene="ompR"
                     /locus_tag="ACHOSC_RS00600"
                     /old_locus_tag="pgaptmp_000120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014646135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator OmpR"
                     /protein_id="HGU4546136.1"
                     /translation="MQENYKILVVDDDMRLRALLERYLTEQGFQVRSVANAEQMDRLL
                     TRESFHLMVLDLMLPGEDGLSICRRLRSQSNPMPIIMVTAKGEEVDRIVGLEIGADDY
                     IPKPFNPRELLARIRAVLRRQANELPGAPSQEEAVIAFGKFKLNLGTREMFREDEPMP
                     LTSGEFAVLKALVSHPREPLSRDKLMNLARGREYSAMERSIDVQISRLRRMVEEDPAH
                     PRYIQTVWGLGYVFVPDGSKA"
     gene            16792..18144
                     /gene="envZ"
                     /locus_tag="ACHOSC_RS00605"
                     /old_locus_tag="pgaptmp_000121"
     CDS             16792..18144
                     /gene="envZ"
                     /locus_tag="ACHOSC_RS00605"
                     /old_locus_tag="pgaptmp_000121"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     EnvZ"
                     /protein_id="HGU4546137.1"
                     /translation="MRRLRFSPRSSFARTLLLIVTLLFASLVTTYLVVLNFAILPSLQ
                     QFNKVLAYEVRMLMTDKLQLEDGTQLVVPPAFRREIYRELGISLYSNEAAEEAGLRWA
                     QHYEFLSHQMAQQLGGPTEVRVEVNKSSPVVWLKTWLSPNIWVRVPLTEIHQGDFSPL
                     FRYTLAIMLLAIGGAWLFIRIQNRPLVDLEHAALQVGKGIIPPPLREYGASEVRSVTR
                     AFNHMAAGVKQLADDRTLLMAGVSHDLRTPLTRIRLATEMMSEQDGYLAESINKDIEE
                     CNAIIEQFIDYLRTGQEMPMEMADLNAVLGEVIAAESGYEREIETALYPGSIDVKMHP
                     LSIKRAVANMVVNAARYGNGWIKVSSGTEPNRAWFQVEDDGPGIAPEQRKHLFQPFVR
                     GDSARTISGTGLGLAIVQRIVDNHNGMLELGTSERGGLSIRAWLPVPVTRAQGTTKEG
                     "
     gene            complement(18220..19842)
                     /gene="pckA"
                     /locus_tag="ACHOSC_RS00610"
                     /old_locus_tag="pgaptmp_000122"
     CDS             complement(18220..19842)
                     /gene="pckA"
                     /locus_tag="ACHOSC_RS00610"
                     /old_locus_tag="pgaptmp_000122"
                     /EC_number="4.1.1.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004612 - phosphoenolpyruvate
                     carboxykinase (ATP) activity [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate carboxykinase (ATP)"
                     /protein_id="HGU4546138.1"
                     /translation="MRVNNGLTPQELEAYGISDVHDIVYNPSYDLLYQEELDPSLTGY
                     ERGVLTNLGAVAVDTGIFTGRSPKDKYIVRDDTTRDTFWWADKGKGKNDNKPLSPETW
                     QHLKGLVTRQLSGKRLFVVDAFCGANPDTRLSVRFITEVAWQAHFVKNMFIRPSDEEL
                     AGFKPDFIVMNGAKCTNPQWKEQGLNSENFVAFNLTERMQLIGGTWYGGEMKKGMFSM
                     MNYLLPLKGIASMHCSANVGEKGDVAVFFGLSGTGKTTLSTDPKRRLIGDDEHGWDDD
                     GVFNFEGGCYAKTIKLSKEAEPEIYNAIRRDALLENVTVREDGTIDFDDGSKTENTRV
                     SYPIYHIDNIVKPVSKAGHATKVIFLTADAFGVLPPVSRLTADQTQYHFLSGFTAKLA
                     GTERGITEPTPTFSACFGAAFLSLHPTQYAEVLVKRMQAAGAQAYLVNTGWNGTGKRI
                     SIKDTRAIIDAILNGSLDNAETFTLPMFNLAIPTELPGVDTKILDPRNTYASPEQWQE
                     KAETLAKLFIDNFDKYTDTPAGAALVAAGPKL"
     gene            20221..21945
                     /gene="yhgE"
                     /locus_tag="ACHOSC_RS00615"
                     /old_locus_tag="pgaptmp_000123"
     CDS             20221..21945
                     /gene="yhgE"
                     /locus_tag="ACHOSC_RS00615"
                     /old_locus_tag="pgaptmp_000123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4153 domain-containing protein"
                     /protein_id="HGU4546139.1"
                     /translation="MDNVELSPATRWGMIATGLLQGLVCYLLIAWLAGKNHSWIVYGV
                     PATVAFSSVLLFSVISFKQKRLWGWLALVFIATTGMSGWLKWQTDGMNPWRAEKAIWD
                     FGCYLLLMAMLLLPWIQQSLRIRNGSSRYSYFYKSVWHNVLILLVIFLANGLTWVVLL
                     LWGELFKLVGIKFFNTLFFATDWFMYLTLGLVTALAVILARTQSRLIDSIQKLFTLIA
                     TGLLPLVSLLTLMFIITLPFTGLSAISRHISAAGLLLTLAFLQLILMAIVRDPQKASL
                     PWTGPLRCLIKTALLVAPLYVFVAAWALWLRVAQYGWTVDRLQGALAVLVLLVWSLGY
                     FVSIVWRNGQNPLVLQGKVNLAVSLLVLVILVLLNSPVLDSMRISVNSHMARYQSGKN
                     TPDQVTIYMLEQSGRYGRAALESLKSDAGFMKDPKRARDLLMALDGEQHLQEQVSEKV
                     LAENVLIAPGSVKPDATFWSALIQDRYNVMTCIEKDACVLVEQDLNSDGQAERILFAF
                     NDDRVIVYGFDSDRKEWDALDMSLLPNEITKEKLLTAAKDGKLGTRPKAWRDLTVDGE
                     TLEINLSK"
     gene            complement(22080..22958)
                     /gene="hslO"
                     /locus_tag="ACHOSC_RS00620"
                     /old_locus_tag="pgaptmp_000124"
     CDS             complement(22080..22958)
                     /gene="hslO"
                     /locus_tag="ACHOSC_RS00620"
                     /old_locus_tag="pgaptmp_000124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417860.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp33 family molecular chaperone HslO"
                     /protein_id="HGU4546140.1"
                     /translation="MPQHDQLHRYLFENFAVRGELVTVSETLQQILENHDYPQPVKNV
                     LAELLVATSLLTATLKFDGDITVQLQGDGPMNLAVINGNNNQQMRGVARVQGEIPENA
                     DLKTLVGNGYVVITITPSEGERYQGVVGLEGDTLAACLEDYFMRSEQLPTRLFIRTGD
                     VDGKPAAGGMLLQVMPAQNAQQDDFDHLATLTETIKTEELLTLPANEVLWRLYHEEEV
                     TVYDPQDVEFKCTCSRERCADALKTLPDEEVDSILTEDGEIDMHCDYCGNHYLFNAMD
                     IAEIRNNASPADPQVH"
     gene            complement(22983..23384)
                     /gene="hslR"
                     /locus_tag="ACHOSC_RS00625"
                     /old_locus_tag="pgaptmp_000125"
     CDS             complement(22983..23384)
                     /gene="hslR"
                     /locus_tag="ACHOSC_RS00625"
                     /old_locus_tag="pgaptmp_000125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated heat shock protein Hsp15"
                     /protein_id="HGU4546141.1"
                     /translation="MKEKPAVEVRLDKWLWAARFYKTRALAREMIEGGKVHYNGQRSK
                     PSKIVELNTTLTLRQGNDERTVIVKAITEQRRPASEAALLYEETAESVEKREKMAMAR
                     KLNALTMPHPDRRPDKKERRDLLRFKHGDSE"
     gene            complement(23395..24063)
                     /gene="yrfG"
                     /locus_tag="ACHOSC_RS00630"
                     /old_locus_tag="pgaptmp_000126"
     CDS             complement(23395..24063)
                     /gene="yrfG"
                     /locus_tag="ACHOSC_RS00630"
                     /old_locus_tag="pgaptmp_000126"
                     /EC_number="3.1.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312268.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GMP/IMP nucleotidase"
                     /protein_id="HGU4546142.1"
                     /translation="MHINIAWQDVDTVLLDMDGTLLDLAFDNYFWQKLVPETWGAKNG
                     VTPQEAMEYMRQQYHDVQHTLNWYCLDYWSEQLGLDICAMTTEMGPRAVLREDTIPFL
                     EALKASGKQRILLTNAHPHNLAVKLEHTGLDAHLDLLLSTHTFGYPKEDQRLWHAVAE
                     ATGLKAERTLFIDDSEAILDAAAQFGIRYCLGVTNPDSGIAEKQYQRHPSLNDYRRLI
                     PSLM"
     gene            complement(24128..26263)
                     /gene="igaA"
                     /locus_tag="ACHOSC_RS00635"
                     /old_locus_tag="pgaptmp_000127"
     CDS             complement(24128..26263)
                     /gene="igaA"
                     /locus_tag="ACHOSC_RS00635"
                     /old_locus_tag="pgaptmp_000127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="intracellular growth attenuator protein IgaA"
                     /protein_id="HGU4546143.1"
                     /translation="MSTIVIFLAALLACSLLAGWLIKVRSRRRQLPWTNAFADAQTRK
                     LTPEERSAVENYLESLTQVLQVPGPTGASAAPISLALNAESNNVMMLTHAITRYGIST
                     DDPNKWRYYLDSVEVHLPPFWEQYINDENTVELIYTDSLPLVISLNGHTLQEYMQETR
                     GYALQPVPSTQASIRGEESEQIELLNIRKETHEEYALSRPRGLREALLIVASFLMFFF
                     CLITPDVFVPWLAGGALLLLGAGLWGLFAPPAKSSLREIHCLRGTPRRWGLFGENDQE
                     QINNISLGIIDLVYPAHWQPYIAQDLGQQTDIDIYLDRHVVRQGRYLSLHDEVKNFPL
                     QHWLRSTIIAAGSLLVLFMLLFWVPLDMPLKFTLSWMKGAQTIEATSVKQLADAGVRV
                     GDTLRISGTGMCNIRTSGTWSAKTNSPFLPFDCSQIIWNDARSLPLPESELVNKATAL
                     TEAVNRQLHPKPEDESRVSASLRSAIQKSGMVLLDDFGDIVLKTADLCSAKDDCVRLK
                     NALVNLGNSKDWDALVKRANAGKLDGVNVLLRPVSAESLDNLVATSTAPFITHETARA
                     AQSLNSPAPGGFLIVSDEGSDFVDQPWPSASLYDYPPQEQWNAFQKLAQMLMHTPFNA
                     EGIVTKIFTDANGTQHIGLHPIPDRSGLWRYLSTTLLLLTMLGSAIYNGVQAWRRYQR
                     HRTRMMEIQAYYESCLNPQLITPSESLIE"
     gene            26583..27143
                     /gene="nudE"
                     /locus_tag="ACHOSC_RS00640"
                     /old_locus_tag="pgaptmp_000128"
     CDS             26583..27143
                     /gene="nudE"
                     /locus_tag="ACHOSC_RS00640"
                     /old_locus_tag="pgaptmp_000128"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ADP compounds hydrolase NudE"
                     /protein_id="HGU4546144.1"
                     /translation="MSKSLQKPTILNVETVARSRLFTVESVDLEFSNGVRRVYERMRP
                     TNREAVMIVPIVDDHLILIREYAVGTESYELGFSKGLIDPGESVYEAANRELKEEVGF
                     GANDLTFLKKLSMAPSYFSSKMNIVVAQDLYPESLEGDEPEPLPQVRWPLAHMMDLLE
                     DPDFNEARNVSALFLVREWLKGQGRV"
     gene            complement(27309..29861)
                     /gene="mrcA"
                     /locus_tag="ACHOSC_RS00645"
                     /old_locus_tag="pgaptmp_000129"
     CDS             complement(27309..29861)
                     /gene="mrcA"
                     /locus_tag="ACHOSC_RS00645"
                     /old_locus_tag="pgaptmp_000129"
                     /EC_number="2.4.99.28"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417855.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan glycosyltransferase/peptidoglycan
                     DD-transpeptidase MrcA"
                     /protein_id="HGU4546145.1"
                     /translation="MKFVKYFLILAVCCILLGAGSIYGLYRYIEPQLPDVATLKDVRL
                     QIPMQIYSADGELIAQYGEKRRIPVTLDQIPPEMVKAFIATEDSRFYEHHGVDPVGIF
                     RAASVALFSGHASQGASTITQQLARNFFLSPERTLMRKIKEVFLAIRIEQLLTKDEIL
                     ELYLNKIYLGYRAYGVGAAAQVYFGKTVDQLTLNEMAVIAGLPKAPSTFNPLYSMDRA
                     VARRNVVLSRMLDEGYITQQQFDQTRTEAINANYHAPEIAFSAPYLSEMVRQEMYNRY
                     GESAYEDGYRIYTTITRKVQQAAQQAVRNNVLDYDMRHGYRGPANVLWKVGESAWDNN
                     KITDTLKALPTYGPLLPAAVTSANPQQATAMLADGSTVALSMEGVRWARPYRSDTQQG
                     PTPRKVTDVLQTGQQIWVRQVGDAWWLAQVPEVNSALVSINPQNGAVMALVGGFDFNQ
                     SKFNRATQALRQVGSNIKPFLYTAAMDKGLTLASMLNDVPISRWDAGAGSDWQPKNSP
                     PQYAGPIRLRQGLGQSKNVVMVRAMRAMGVDYAAEYLQRFGFPAQNIVHTESLALGSA
                     SFTPMQVARGYAVMANGGFLVDPWFISKIENDQGGVIFEAKPKVACPECDIPVIYGDT
                     QKSNVLENNDVEDVAISREQQNVSVPMPQLEQANQALVAKTGAQEYAPHVINTPLAFL
                     IKSALNTNIFGEPGWQGTGWRAGRDLQRRDIGGKTGTTNSSKDAWFSGYGPGVVTSVW
                     IGFDDHRRNLGHTTASGAIKDQISGYEGGAKSAQPAWDAYMKAVLEGVPEQPLTPPPG
                     IVTVNIDRSTGQLANGGNSREEYFIEGTQPTQQAVHEVGTTIIDNGEAQELF"
     gene            29981..30760
                     /gene="hofM"
                     /locus_tag="ACHOSC_RS00650"
                     /old_locus_tag="pgaptmp_000130"
     CDS             29981..30760
                     /gene="hofM"
                     /locus_tag="ACHOSC_RS00650"
                     /old_locus_tag="pgaptmp_000130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417854.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA utilization protein HofM"
                     /protein_id="HGU4546146.1"
                     /translation="MAFKIWQIGLHLQQQEAVAVAIVRGAKECFLQRWWRLPLENDII
                     KDGRIVDAQQLAKTLLPWSRELPQRHHIMLAFPASRTLQRSFPRPSMSLGEREQTAWL
                     SGTMARELDMDPDSLRFDYSEDSLSPAYNVTAAQSKELATLLTLAERLRVHVSAITPD
                     ASALQRFLPFLPSHQQCLAWRDNEQWLWATRYSWGRKLAVGMTSAKELAAALSVDPDS
                     VAICGEGGFDPWEAVSVRQPPLPPPGGDFAIALGLALGKAY"
     gene            30760..31299
                     /gene="hofN"
                     /locus_tag="ACHOSC_RS00655"
                     /old_locus_tag="pgaptmp_000131"
     CDS             30760..31299
                     /gene="hofN"
                     /locus_tag="ACHOSC_RS00655"
                     /old_locus_tag="pgaptmp_000131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA utilization protein HofN"
                     /protein_id="HGU4546147.1"
                     /translation="MNPPINFLPWRQQRRTAFLRFWLLMFVAPLLLAVGITLILRLTG
                     SAEARIDAVLLQAEQQLARSLQITKPRLLEQQQLREQRSQRQRQRQFTRDWQSALEAL
                     AALLPEHAWLTTISWQQGTLEIKGLTTSITALNALETSLRQDASFHLNQRGATQQDAQ
                     GRWQFEYQLTRKVSDEHVL"
     gene            31283..31723
                     /gene="hofO"
                     /locus_tag="ACHOSC_RS00660"
                     /old_locus_tag="pgaptmp_000132"
     CDS             31283..31723
                     /gene="hofO"
                     /locus_tag="ACHOSC_RS00660"
                     /old_locus_tag="pgaptmp_000132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA utilization protein HofO"
                     /protein_id="HGU4546148.1"
                     /translation="MNMFFDWWFATSPRLRQFCWAFWLLMLVTLIFLSSTHHEERDAL
                     IRLRASHHQQWATLYRLVDTTPFSEEKTLPFSPLDFQLPGAQLVSWHPSAQGGELALK
                     TLWEAVPSAFTRLAERNVSVSRFSLSVEGDDLLFTLQLETPHEG"
     gene            31713..32117
                     /gene="hofP"
                     /locus_tag="ACHOSC_RS00665"
                     /old_locus_tag="pgaptmp_000133"
     CDS             31713..32117
                     /gene="hofP"
                     /locus_tag="ACHOSC_RS00665"
                     /old_locus_tag="pgaptmp_000133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417851.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA utilization protein HofP"
                     /protein_id="HGU4546149.1"
                     /translation="MRVKRWLLAGIALCLLTGMRDPFKPPEDLCRISELSQWRYQGMV
                     GRGEHIIGVIKDGQKKWRRVQQNDVLENGWTILQLTPDALTLGTGTNCEPPQWLWQRQ
                     GDTNEAMDSRTTVDADTRRTGGKAAKSDADGG"
     gene            32029..33267
                     /gene="hofQ"
                     /locus_tag="ACHOSC_RS00670"
                     /old_locus_tag="pgaptmp_000134"
     CDS             32029..33267
                     /gene="hofQ"
                     /locus_tag="ACHOSC_RS00670"
                     /old_locus_tag="pgaptmp_000134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA uptake porin HofQ"
                     /protein_id="HGU4546150.1"
                     /translation="MKQWIAALLLMLIPGVQAAKPQKVTLMVDDVPVAQVLQALAEQE
                     KLNLVVSPDVSGTVSLHLTDVPWKQALQTVVKSAGLITRQEDNILSVHSIAWQNNNIA
                     RQEAEQARAQANLPLENRSITLQYADAGELAKAGEKLLSAKGSMTVDKRTNRLLLRDN
                     KTALSALEQWVAQMDLPVGQVELSAHIVTINEKSLRELGVKWTLADAQHAGGVGQVTT
                     LGSDLSVATATTHVGFNIGRINGRLLDLELSALEQKQQLDIIASPRLLASHLQPASIK
                     QGSEIPYQVSSGESGATSVEFKEAVLGMEVTPTVLQKGRIRLKLHISQNVPGQVLQQA
                     DGEVLAIDKQEIETQVEVKSGETLALGGIFTRKNKSGQDSVPLLGDIPWFGQLFRHDG
                     KEDERRELVVFITPRLVSSE"
     gene            33668..34189
                     /gene="aroK"
                     /locus_tag="ACHOSC_RS00675"
                     /old_locus_tag="pgaptmp_000135"
     CDS             33668..34189
                     /gene="aroK"
                     /locus_tag="ACHOSC_RS00675"
                     /old_locus_tag="pgaptmp_000135"
                     /EC_number="2.7.1.71"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004709337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate kinase AroK"
                     /protein_id="HGU4546151.1"
                     /translation="MAEKRNIFLVGPMGAGKSTIGRQLAQQLNMEFYDSDQEIEKRTG
                     ADVGWVFDLEGEEGFRDREEKVINELTEKQGIVLATGGGSVKSRETRNRLSARGVVVY
                     LETTIEKQLARTQRDKKRPLLHVETPPREVLEALANERNPLYEEIADVTIRTDDQSAK
                     VVANQIIHMLESN"
     gene            34246..35334
                     /gene="aroB"
                     /locus_tag="ACHOSC_RS00680"
                     /old_locus_tag="pgaptmp_000136"
     CDS             34246..35334
                     /gene="aroB"
                     /locus_tag="ACHOSC_RS00680"
                     /old_locus_tag="pgaptmp_000136"
                     /EC_number="4.2.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003856 - 3-dehydroquinate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-dehydroquinate synthase"
                     /protein_id="HGU4546152.1"
                     /translation="MERIVVTLGERSYPITIASGLFNEPASFLPLKSGEQVMLVTNET
                     LAPLYLDKVRGVLEQAGVNVDSVILPDGEQYKSLAVLDTVFTALLQKPHGRDTTLVAL
                     GGGVVGDLTGFAAASYQRGVRFIQVPTTLLSQVDSSVGGKTAVNHPLGKNMIGAFYQP
                     ASVVVDLDCLKTLPPRELASGLAEVIKYGIILDGAFFNWLEENLDALLRLDGPAMAYC
                     IRRCCELKAEVVAADERETGLRALLNLGHTFGHAIEAEMGYGNWLHGEAVAAGMVMAA
                     RTSERLGQFSSAETQRIITLLKRAGLPVNGPREMSAQAYLPHMLRDKKVLAGEIRLIL
                     PLAIGKSEVRSGVSHELVLNAIADSQSA"
     gene            35426..36712
                     /gene="damX"
                     /locus_tag="ACHOSC_RS00685"
                     /old_locus_tag="pgaptmp_000137"
     CDS             35426..36712
                     /gene="damX"
                     /locus_tag="ACHOSC_RS00685"
                     /old_locus_tag="pgaptmp_000137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA];
                     GO_process: GO:0032506 - cytokinetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein DamX"
                     /protein_id="HGU4546153.1"
                     /translation="MDEFKPEDELKPDPSDRRTGRSRQSSERSERTERGEPQINFDDI
                     ELDDTDDRRPTRAQKERNEEPEIEEEIDESEDETVDEERVERRPRKRKKAASKPASRQ
                     YMMMGVGILVLLLLIIGIGSALKAPSTSSSDQTASGEKSIDLAGNATDQANGVQPAPG
                     TTSAENTQQDVSLPPISSTPTQGQTPAATDGQQRVEVQGDLNNALTQPQNQQQLNNVA
                     VNSTLPTEPATVAPVRNGNASRDTAKTQTAERPATTRPARQQAVIEPKKPQATVKTEP
                     KPVAQTPKRTEPAAPVASTKAPAATSAPAPKETATTAPVQTASPAQTTATPAAGGKTA
                     GNVGSLKSAPSSHYTLQLSSSSNYDNLNGWAKKENLKNYVVYETTRNGQPWYVLVSGV
                     YASKEEAKKAVSTLPADVQAKNPWAKPLRQVQADLK"
     gene            36819..37655
                     /gene="dam"
                     /locus_tag="ACHOSC_RS00690"
                     /old_locus_tag="pgaptmp_000138"
     CDS             36819..37655
                     /gene="dam"
                     /locus_tag="ACHOSC_RS00690"
                     /old_locus_tag="pgaptmp_000138"
                     /EC_number="2.1.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0009007 - site-specific
                     DNA-methyltransferase (adenine-specific) activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenine-specific DNA-methyltransferase"
                     /protein_id="HGU4546154.1"
                     /translation="MKKNRAFLKWAGGKYPLLDDIKRHLPKGECLVEPFVGAGSVFLN
                     TDFSRYILADINSDLISLYNIVKMRTDEYVQAARELFVPETNCAEVYYQFREEFNKSQ
                     DPFRRAVLFLYLNRYGYNGLCRYNLRGEFNVPFGRYKKPYFPEAELYHFAEKAQNAFF
                     YCESYADSMARADDASVVYCDPPYAPLSATANFTAYHTNSFTLEQQAHLAEIAEGLVE
                     RHIPVLISNHDTMLTREWYQRAKLHVVKVRRSISSNGGTRKKVDELLALYKPGVVSPA
                     KK"
     gene            37673..38350
                     /gene="rpe"
                     /locus_tag="ACHOSC_RS00695"
                     /old_locus_tag="pgaptmp_000139"
     CDS             37673..38350
                     /gene="rpe"
                     /locus_tag="ACHOSC_RS00695"
                     /old_locus_tag="pgaptmp_000139"
                     /EC_number="5.1.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004750 - D-ribulose-phosphate 3-epimerase
                     activity [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribulose-phosphate 3-epimerase"
                     /protein_id="HGU4546155.1"
                     /translation="MKQYLIAPSILSADFARLGEDTAKALAAGADVVHFDVMDNHYVP
                     NLTIGPMVLKSLRNYGITAPIDVHLMVKPVDRIVPDFAAAGASIITFHPEASEHVDRT
                     LQLIKENGCKAGLVFNPATPLSYLDYVMDKLDVILLMSVNPGFGGQSFIPQTLDKLRE
                     VRRRIDESGFDIRLEVDGGVKVNNIGEIAAAGADMFVAGSAIFDQPDYKKVIDEMRSE
                     LAKVSHE"
     gene            38343..39101
                     /gene="gph"
                     /locus_tag="ACHOSC_RS00700"
                     /old_locus_tag="pgaptmp_000140"
     CDS             38343..39101
                     /gene="gph"
                     /locus_tag="ACHOSC_RS00700"
                     /old_locus_tag="pgaptmp_000140"
                     /EC_number="3.1.3.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008967 - phosphoglycolate phosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycolate phosphatase"
                     /protein_id="HGU4546156.1"
                     /translation="MNKFEDIRGVAFDLDGTLVDSAPGLAAAVDMALYALELPVAGEE
                     RVITWIGNGADVLMERALTWARQERATQRKTMGKPPVDDDIPAEEQVRILRKLFDRYY
                     GEVAEEGTFLFPHVADTLGALQAKGLPLGLVTNKPTPFVAPLLEALDIAKYFSVVIGG
                     DDVQNKKPHPDPLLLVAERMGIAPQQMLFVGDSRNDIQAAKAAGCPSVGLTYGYNYGE
                     AIDLSQPDVIYQSINDLLPALGLPHSENQESKND"
     gene            39094..40098
                     /gene="trpS"
                     /locus_tag="ACHOSC_RS00705"
                     /old_locus_tag="pgaptmp_000141"
     CDS             39094..40098
                     /gene="trpS"
                     /locus_tag="ACHOSC_RS00705"
                     /old_locus_tag="pgaptmp_000141"
                     /EC_number="6.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004830 - tryptophan-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006436 - tryptophanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan--tRNA ligase"
                     /protein_id="HGU4546157.1"
                     /translation="MTKPIVFSGAQPSGELTIGNYMGALRQWVNMQDDYHCIYCIVDQ
                     HAITVRQDAQKLRKATLDTLALYLACGIDPEKSTIFVQSHVPEHAQLGWALNCYTYFG
                     ELSRMTQFKDKSARYAENINAGLFDYPVLMAADILLYQTNLVPVGEDQKQHLELSRDI
                     AQRFNALYGEIFKVPEPFIPKSGARVMSLLEPTKKMSKSDDNRNNVIGLLEDPKSVVK
                     KIKRAVTDSDEPPVVRYDVQNKAGVSNLLDILSAVTGQSIPELEKQFEGKMYGHLKGE
                     VADAVSGMLTELQERYHRFRNDEAFLQQVMKDGAEKASVHASRTLKAVYEAIGFVAKP
                     "
     gene            40367..41272
                     /gene="yhfZ"
                     /locus_tag="ACHOSC_RS00710"
                     /old_locus_tag="pgaptmp_000142"
     CDS             40367..41272
                     /gene="yhfZ"
                     /locus_tag="ACHOSC_RS00710"
                     /old_locus_tag="pgaptmp_000142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator YhfZ"
                     /protein_id="HGU4546158.1"
                     /translation="MRRTFIKKEGVVITTLARYLLGEKCGNRLKTIDELATECRSSVG
                     LTQAALKTLESSGAIRIERRGRNGSYLVEMDNKALLSHVDINNVVCAMPLPYTRLYEG
                     LASGLKAQFDGIPFYYAHMRGADIRVECLLNGVYDMAVVSRLAAESYLTQKGFCLALE
                     LGPHTYVGEHQLICRKGESANVKRVGLDNRSADQKIMTDVFFGDSDVERVDLSYHESL
                     QRIVKGDVDAVIWNVVAENELTMLGLEATPLTDDPRFLQATEAVVLTRVDDYPMQQLL
                     RAVVDKHALLAHQQRVVSGEQEPSY"
     gene            41289..41651
                     /gene="yhfY"
                     /locus_tag="ACHOSC_RS00715"
                     /old_locus_tag="pgaptmp_000143"
     CDS             41289..41651
                     /gene="yhfY"
                     /locus_tag="ACHOSC_RS00715"
                     /old_locus_tag="pgaptmp_000143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417841.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HGU4546159.1"
                     /translation="METRLNLLCEAGVIDKDICKGMMQVVNVLETECHLPVRSEQGTM
                     AMTHMASALMRSRRGEEIEPLDNELLAELAQSSHWQAVVQLHQVLLKEFALEVNPCEE
                     GYLLANLYGLWMAANEEV"
     gene            41735..42898
                     /gene="yhfX"
                     /locus_tag="ACHOSC_RS00720"
                     /old_locus_tag="pgaptmp_000144"
     CDS             41735..42898
                     /gene="yhfX"
                     /locus_tag="ACHOSC_RS00720"
                     /old_locus_tag="pgaptmp_000144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhfX family PLP-dependent enzyme"
                     /protein_id="HGU4546160.1"
                     /translation="MFVEALKRQNPALISAVLSLWQQGKIAPDSWVIDVDQVLENGKR
                     LIETARLYGIELYLMTKQFGRNPWLAEKLLALGYSGIVAVDYKEARVMRRAGLSVAHQ
                     GHLVQIPCHQVADAVEQGTDVITVFTLDKAREVSAAAVKAGRVQFVLLKVYSDDDFLY
                     PGQESGFVQHSLHEVVAEIKKLPGLHLAGLTHFPCLLWDEAAGKVLPTPNLHTLVQAR
                     DQLAKSGIAIEQLNAPSATSCTSLPMLAEYGVTHAEPGHALTGTIPANQQGDQPEYIA
                     MLWLSEISHHFRGDSYCYGGGYYRRGHAQHALVFTPENQKITETNLKTVDDSSIDYTL
                     PLAGEFPVSSAVVLCFRTQIFVTRSDVVLVSGIHRGEPKIVGRYDSLGNSLGA"
     gene            42898..44124
                     /gene="yhfW"
                     /locus_tag="ACHOSC_RS00725"
                     /old_locus_tag="pgaptmp_000145"
     CDS             42898..44124
                     /gene="yhfW"
                     /locus_tag="ACHOSC_RS00725"
                     /old_locus_tag="pgaptmp_000145"
                     /EC_number="5.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008973 - phosphopentomutase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopentomutase"
                     /protein_id="HGU4546161.1"
                     /translation="MARFVVLVIDSFGVGAMKDVTLVRPQDAGANTCGHILSQLPHLQ
                     LPALEKLGLINALGYAPGDMQPSDSATWGVAELQHEGGDTFMGHQEILGTRPLPPLRM
                     PFRDVIGRVEQALVSAGWQVERRGDDLQFLWVNQAVAIGDNLEADLGQVYNITANLSV
                     ISFDDAIKIGRIVREQVQVGRVITFGGLLTDSQRILDAAESKEGRFIGINAPRSGAYD
                     NGFQVVHMGYGVDEKVQVPQKLYEAGVPTVLVGKVADIVSNPYGVSWQNLVDSQRIMD
                     ITLDEFNTHPTAFICINIQETDLAGHAEDVARYAERLQVVDRNLARLVEAMQPDDCLV
                     VMADHGNDPTIGHSHHTREVVPVLVYQQGLVHTQLGVRTTLSDVGATVCEFFRAPPPQ
                     NGRSFLSSLRFAGDTL"
     gene            44121..44999
                     /gene="php"
                     /locus_tag="ACHOSC_RS00730"
                     /old_locus_tag="pgaptmp_000146"
     CDS             44121..44999
                     /gene="php"
                     /locus_tag="ACHOSC_RS00730"
                     /old_locus_tag="pgaptmp_000146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotriesterase-related protein"
                     /protein_id="HGU4546162.1"
                     /translation="MSIDPTGYTLAHEHLHIDLSGFKNNVDCRLDQYAFICQEMNDLM
                     TRGVRNVIEMTNRYMGRNAQFMLDVMRETGINVVACTGYYQDAFFPEHVATRSVQELA
                     QEMVDEIEQGIDGTDLKAGIIAEIGSSEGKITPLEEKVFIAAALAHNQTGRPISTHTS
                     FSTMGLEQLALLQAHGVDLSRVTVGHCDLKDNLDNILKMIDLGAYVQFDTIGKNSYYP
                     DEKRIAMLHALRDRGLLNRVMLSMDITRRSHLKANGGYGYDYLLTTFIPQLRQSGFSQ
                     ADVDVMLRENPSQFFQ"
     gene            45010..45363
                     /gene="yhfU"
                     /locus_tag="ACHOSC_RS00735"
                     /old_locus_tag="pgaptmp_000147"
     CDS             45010..45363
                     /gene="yhfU"
                     /locus_tag="ACHOSC_RS00735"
                     /old_locus_tag="pgaptmp_000147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417837.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2620 domain-containing protein"
                     /protein_id="HGU4546163.1"
                     /translation="MKKIGVAGLQREQIKKTIEATAPGSFEVFIHNDMEAAMKVKSGQ
                     LDYYIGACNTGAGAALSIAIAVIGYNKSCTIAKPGIKAKDEHIAKMIAEGKVAFGLSV
                     EHVEHAIPMLINHLK"
     gene            45375..46679
                     /gene="yhfT"
                     /locus_tag="ACHOSC_RS00740"
                     /old_locus_tag="pgaptmp_000148"
     CDS             45375..46679
                     /gene="yhfT"
                     /locus_tag="ACHOSC_RS00740"
                     /old_locus_tag="pgaptmp_000148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhfT family protein"
                     /protein_id="HGU4546164.1"
                     /translation="MDLYIQIIVVACLTGMTSLLAHRSAAVFHDGIRPILPQLIEGYM
                     NRREAGSIAFGLSIGFVASVGISFTLKTGLLNAWLLFLPTDILGVLAINSLMAFGLGA
                     IWGVLILTCLLPVNQLLTALPVDVLGSLGELSSPVVSAFALFPLVAIFYQFGWKQSLV
                     AAVVVLMTRVVVVRYFPHLNPESIEIFIGMVMLLGIAITHDLRHRDENDIDASGLSVF
                     EERTSRIIKNLPYIAIVGALIAAVASMKIFAGSEVSIFTLEKAYSAGVTPEQSQTLIN
                     QAALAEFMRGLGFVPLIATTALATGVYAVAGFTFVYAVGYLSPNPMVAAVLGAVVISA
                     EVLLLRSIGKWLGRYPSVRNASDNIRNAMNMLMEVALLVGSIFAAIKMAGYTGFSIAV
                     AIYFLNESLGRPVQKMAAPVVAVMITGILLNVLYWLGLFVPA"
     gene            46691..47776
                     /gene="yhfS"
                     /locus_tag="ACHOSC_RS00745"
                     /old_locus_tag="pgaptmp_000149"
     CDS             46691..47776
                     /gene="yhfS"
                     /locus_tag="ACHOSC_RS00745"
                     /old_locus_tag="pgaptmp_000149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HGU4546165.1"
                     /translation="MKTFPLQSLTIIEAQQKQFALVDSICRHFPGSEFLTGGDLGLTP
                     GLNQPRITQRVEQVLADAFHAQAAALVQGAGTGAIRAGLAALLKPGQRLLVHDAPVYP
                     TTRVIIEQMGLTLITVDFNDLSALKQVVDEQQPDAALVQHTRQQPQDSYVLADVLATL
                     RAAGVPALTDDNYAVMKVARIGCECGANVSTFSCFKLFGPEGVGAVVGDADVINRIRA
                     TLYSGGSQIQGAQALEVLRGLVFAPVMHAVQAGVSERLLALLNGGAVPEVKSAVIANA
                     QSKVLIVEFHQPIAARVLEEAQKRGALPYPVGAESKYEIPPLFYRLSGTFRQANPQSE
                     HCAIRINPNRSGEETVLRILRESIASI"
     gene            complement(47928..48659)
                     /gene="frlR"
                     /locus_tag="ACHOSC_RS00750"
                     /old_locus_tag="pgaptmp_000150"
                     /gene_synonym="yhfR"
     CDS             complement(47928..48659)
                     /gene="frlR"
                     /locus_tag="ACHOSC_RS00750"
                     /old_locus_tag="pgaptmp_000150"
                     /gene_synonym="yhfR"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417834.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator FrlR"
                     /protein_id="HGU4546166.1"
                     /translation="MSATDRYSHQLLYATVRQRLLDDIAQGVYQAGQQIPTENELCTQ
                     YNVSRITIRKAISDLVADGVLIRWQGKGTFVQSQKVENALLTVSGFTDFGVSQGKATK
                     EKVIEQERVSAAPFCEKLNIPGNSEVFHLCRVMYLDKEPLFIDSSWIPLSRYPDFDEI
                     YVEGSSTYQLFQERFDTRVVSDKKTIDIFAATRPQAKWLKCELGEPLFRISKIAFDQN
                     DKPVHVSELFCRANRITLTIDNKRH"
     gene            complement(48759..49544)
                     /gene="frlD"
                     /locus_tag="ACHOSC_RS00755"
                     /old_locus_tag="pgaptmp_000151"
     CDS             complement(48759..49544)
                     /gene="frlD"
                     /locus_tag="ACHOSC_RS00755"
                     /old_locus_tag="pgaptmp_000151"
                     /EC_number="2.7.1.218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructoselysine 6-kinase"
                     /protein_id="HGU4546167.1"
                     /translation="MKTLATIGDNCVDIYPQLNKAFSGGNAVNVAVYCTRYGIQPGCI
                     TWVGDDDYGTKLKQDLARMGVDISHVHTKHGVTAQTQVELHDNDRVFGDYTEGVMADF
                     ALSEEDYAWLAQYDIVHAAIWGHAEDAFPQLHAAGKLTAFDFSDKWDSSLWQTLVPHL
                     DFAFASAPQEDEALRLKMKAIVARGAGVVIVTLGENGSIAWDGAQFWRQAPEPVTVID
                     TMGAGDSFIAGFLCGWSAGMTLPQAMAQGTACAAKTIQYHGAW"
     gene            complement(49541..50371)
                     /gene="frlC"
                     /locus_tag="ACHOSC_RS00760"
                     /old_locus_tag="pgaptmp_000152"
     CDS             complement(49541..50371)
                     /gene="frlC"
                     /locus_tag="ACHOSC_RS00760"
                     /old_locus_tag="pgaptmp_000152"
                     /EC_number="5.1.3.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructoselysine 3-epimerase"
                     /protein_id="HGU4546168.1"
                     /translation="MKTGMFTCGHQRLPIEHAFRDASELGYDGIEIWGGRPHAFAPDL
                     KAGGIKQIKALAQTYQMPIIGYTPETNGYPYNMMLGDEHMRRESLDMIKLAMDMAKEM
                     NAGYTLISAAHAGYLTPPNVIWGRLAENLSELCEYAENIGMDLILEPLTPYESNVVCN
                     ANDVLHALALVPSPRLFSMVDICAPYVQAEPVMSYFDKLGDKLRHLHIVDSDGASDTH
                     YIPGEGKMPLRELMRDIIDRGYEGYCTVELVTMYMNEPRLYARQALERFRALLPEDER
                     "
     gene            complement(50421..51443)
                     /gene="frlB"
                     /locus_tag="ACHOSC_RS00765"
                     /old_locus_tag="pgaptmp_000153"
     CDS             complement(50421..51443)
                     /gene="frlB"
                     /locus_tag="ACHOSC_RS00765"
                     /old_locus_tag="pgaptmp_000153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417830.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructoselysine 6-phosphate deglycase"
                     /protein_id="HGU4546169.1"
                     /translation="MLDIDKSTVDFLVTENMVQEVEKVLSHDVPLVHTIVEEMVKRDI
                     DRIYFVACGSPLNAAQTAKHLADRFSDLQVYAISGWEFCDNTPYRLDDRCAVIGVSDY
                     GKTEEVIKALELGRACGALTAAFTKRADSPITSAAEFSIDYQADCIWEIHLLLCYSVV
                     LEMITRLAPNAEIGKIKNDLKQLPNALGQLVRTWEEKGRQLGELASQWPMIYTVAAGP
                     LRPLGYKEGIVTLMEFTWTHGCVIESGEFRHGPLEIVEPGVPFLFLLGNDESRHTTER
                     AINFVKQRTDNVIVIDYAEISQGLHPWLAPFLMFVPMEWLCYYLSIYKDHNPDERRYY
                     GGLVEY"
     gene            complement(51464..52801)
                     /gene="frlA"
                     /locus_tag="ACHOSC_RS00770"
                     /old_locus_tag="pgaptmp_000154"
     CDS             complement(51464..52801)
                     /gene="frlA"
                     /locus_tag="ACHOSC_RS00770"
                     /old_locus_tag="pgaptmp_000154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417829.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructoselysine/psicoselysine transporter"
                     /protein_id="HGU4546170.1"
                     /translation="MGSQELQRKLGFWAVLAIAVGTTVGSGIFVSVGEVAKAAGTPWL
                     TVLAFVIGGLIVIPQMCVYAELSTAYPENGADYVYLKNAGSRPLAFLSGWASFWANDA
                     PSLSIMALAIVSNLGFLTPIDPLLGKFIAAGLIIAFMLLHLRSVEGGAAFQTLITIAK
                     IIPFTIVIGLGIFWFKAENFAAPTTTAIGATGSFMALLAGISATSWSYTGMASICYMT
                     GEIKNPGKTMPRALIGSCLLVLVLYTLLALVISGLMPFDKLANSETPISDALTWIPAL
                     GSTAGIFVAITAMIVILGSLSSCVMYQPRLEYAMAKDNLFFKCFGHVHPKYNTPDVSI
                     ILQGALGIFFIFVSDLTSLLGYFTLVMCFKNTLTFGSIIWCRKRDDYKPLWRTPAFGL
                     MTTLAIASSLILVASTFVWAPIPGLICAVIVIATGLPAYAFWAKRSRQLNALS"
     gene            complement(53096..53224)
                     /locus_tag="ACHOSC_RS00775"
                     /old_locus_tag="pgaptmp_000155"
     CDS             complement(53096..53224)
                     /locus_tag="ACHOSC_RS00775"
                     /old_locus_tag="pgaptmp_000155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020240495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4223 family protein"
                     /protein_id="HGU4546171.1"
                     /translation="MATLTACTGHIENRDKNCSYDYLLHPAISISKIIGGCGPTAQ"
BASE COUNT        12504 a        13459 c        14661 g        12602 t
ORIGIN      
        1 cctttactta atttatagag ttaccttccg ctttttgaaa atacgcaacg gccatttttt
       61 gcacttagat acagattttc tgcgctgtat tgcattgatt tgctgctaat cctgtggttt
      121 gcactagctt taagtggttg agatcacatt tccttgctca tccccgcaac tcctccctgc
      181 ctaatccccc gcaggatgag gaaggtcaac atcgagcctg gcaaactagc gataacgttg
      241 tgttgaaaat ctaagaaaag tggaactcct atgtcacaac ctatttttaa cgataagcaa
      301 tttcaggaag cgctttcacg tcagtggcag cgttatggct taaattctgc ggctgaaatg
      361 actcctcgcc agtggtggct agcagtgagt gaagcactgg ccgaaatgct gcgtgctcag
      421 ccattcgcca agccggtggc gaatcagcga catgttaact acatctcaat ggagtttttg
      481 attggtcgcc tgacgggcaa caacctgttg aatctcggct ggtatcagga tgtacaggat
      541 tcgttgaagg cttatgacat caatctgacg gacctgctgg aagaagagat cgacccggcg
      601 ctgggtaacg gtggtctggg acgtctggcg gcgtgcttcc tcgactcaat ggcaactgtc
      661 ggtcagtctg cgacgggtta cggtctgaac tatcaatatg gtttgttccg ccagtctttt
      721 gtcgatggca aacaggttga agcgccggat gactggcatc gcagtaacta cccgtggttc
      781 cgccacaacg aagcactgga tgtgcaggta gggattggcg gtaaagtgac gaaagacgga
      841 cgctgggagc cggagtttac cattaccggt caagcgtggg atctccccgt tgtcggctat
      901 cgtaatggcg tggcgcagcc gctgcgtctg tggcaggcga cgcacgcgca tccgtttgat
      961 ctgactaaat ttaacgacgg tgatttcctg cgtgccgaac agcagggcat caatgcggaa
     1021 aaactgacca aagttctcta tccaaacgac aaccatactg ccggtaaaaa gctgcgcttg
     1081 atgcagcagt acttccagtg tgcctgttcg gtagcggata ttttgcgtcg ccatcatctg
     1141 gcggggcgta aactgcacga actggcggat tacgaagtta ttcagctgaa cgatacccac
     1201 ccaactatcg cgattccaga actgctgcgc gtgctgatcg atgagcacca gatgagctgg
     1261 gatgacgcct gggccattac cagcaaaact ttcgcttaca ccaaccatac cctgatgcca
     1321 gaagcgctgg aacgctggga tgtgaaactg gtgaaaggct tactgccgcg ccatatgcag
     1381 attattaacg aaattaatac tcgctttaaa acgctggtgg agaaaacctg gccgggcgat
     1441 gaaaaagtgt gggccaaact ggcggtggtg cacgataaac aagtgcatat ggcgaacctg
     1501 tgtgtggttg gcggtttcgc ggtgaacggt gttgcggcgc tgcactcgga tctggtggtg
     1561 aaagatctgt tcccggaata tcaccagcta tggccgaaca aattccataa cgtcaccaac
     1621 ggtattaccc cacgtcgctg gatcaaacag tgcaaccctg cactggcggc tctgttggat
     1681 aaatcactgc aaaaagagtg ggctaacgat ctcgatcagc tgatcaatct ggaaaaattc
     1741 gctgatgatg cgaaattccg tcagcaatat cgcgagatca agcaggcgaa taaagtccgt
     1801 ctggcggagt ttgtgaaagt tcgtaccggt attgagatca atccacaggc gattttcgat
     1861 attcagatca aacgtctgca tgagtacaaa cgccagcacc tgaatctgct gcatattctg
     1921 gcgttgtaca aagaaattcg tgaaaacccg caggctgatc gcgtaccgcg cgtcttcctc
     1981 ttcggcgcga aagcggcacc gggctactac ctggcgaaga atattatctt tgcgatcaac
     2041 aaagtggctg acgtgatcaa caacgatcca ctggttggcg ataagttgaa ggtggtgttc
     2101 ctgccggatt attgcgtttc ggcggcggaa aaactgatcc cggcggcgga tatctccgaa
     2161 caaatttcga ctgcaggtaa agaagcttcc ggtaccggca atatgaaact ggcgctcaat
     2221 ggtgcgctta ctgtcggtac gctggatggt gcgaacgttg aaatcgccga gaaagtcggt
     2281 gaagaaaata tctttatttt tggtcatacc gtggaacaag tgaaggctat tctggccaaa
     2341 ggctacgacc cggtgaaatg gcggaagaaa gataaggtgc tggacgcagt attgaaagag
     2401 ctggaaagcg gtaaatacag cgacggcgat aagcatgcct tcgaccagat gctgcacagt
     2461 atcggcaaac aaggcggcga tccgtatctg gtgatggcgg atttcgcagc ctatgttgag
     2521 gcacaaaagc aggtggatgt gctgtaccgc gaccaggagg cctggactcg cgcggcgatc
     2581 ctcaataccg cccgctgcgg tatgtttagc tcggatcgct ctattcgcga ttatcaggct
     2641 cgtatctggc aggcaaaacg ctaaggaagc tcgatggaaa gcaaacgtct ggataatgcc
     2701 gcgctggcgg cggggattag ccccaattac atcaatgccc acggtaaacc gcagtcgatt
     2761 agcgccgaaa ccaaacggcg tttgcttgac gcgatgcatc aacgtaccgc cacgaaagtg
     2821 gcggtaacgc cagtcccgaa tgtcatggtt tataccagcg gcaaaaaaat gccgatggtg
     2881 gtggagggca gcggcgaata tagctggctg ctgaccaccg aagaaggaac acagtacaaa
     2941 ggccatgtaa cggggggcaa agcgttcaat ctaccgacga agctgccgga aggttatcac
     3001 acgctgacac tcacccagga cgaccagcgc gcgcattgcc gggtgattgt cgccccgaaa
     3061 cgctgttacg aaccgcaggc gttgctgaat aaacaaaagc tgtggggtgc ctgcgttcag
     3121 ctttatacgc tgcgatcgga aaaaaactgg ggtattgggg attttggcga tctcaaagcg
     3181 atgctggtgg atgtggcaaa acgtggcggg tcgttcatcg gcctgaaccc gattcatgcg
     3241 ctctatccgg caaatccgga gagtgccagc ccatacagcc cgtcttctcg ccgttggctg
     3301 aatgtgattt atatcgacgt taacgccgtt gaagatttcc atcttagcga agaggctcag
     3361 gcctggtggc agttgccgac cacgcaacag acgctgcaac aggcgcgcga tgccgactgg
     3421 gtcgattact ccacggttac cgccctaaaa atgacagcat tacgaatggc gtggaaaggt
     3481 ttcgcgcaac gtgatgatga gcagatgacc gcgtttcgcc agtttgttgc agagcagggc
     3541 gacagcctgt tctggcaggc agcctttgat gcgctacatg cccagcaagt gaaagaggac
     3601 gaaatgcgct ggggctggcc tgcatggcca gagatgtatc agaacgtgga ttcaccagaa
     3661 gtgcgtcagt tctgcgaaga acatcgtgat gacgtcgatt tttatctctg gttgcagtgg
     3721 ctggcttaca gccagtttgc cgcctgctgg gagataagcc agggctatga aatgccgatt
     3781 ggcttgtatc gtgatctggc ggttggcgta gcggaaggtg gggcggaaac ctggtgtgac
     3841 cgtgaactat attgcctgaa agcgtcggtt ggcgcgccgc cggatatcct cggcccgttg
     3901 gggcagaact ggggattacc gccaatggac ccgcatatca tcaccgcgcg tgcctatgaa
     3961 ccgtttatcg agctgttgcg tgccaatatg caaaactgcg gcgcattacg aattgaccat
     4021 gtgatgtcga tgctgcgttt gtggtggata ccgtatggcg agacggcaga tcagggcgcg
     4081 tatgttcact atccggtgga tgatctgctc tcgattctgg cactcgaaag taaacgtcat
     4141 cgctgtatgg tgattggtga agatctcggt accgtaccgg tagagattgt cggtaagctg
     4201 cgcagcagcg gtgtgtactc ctacaaagtg ctctatttcg aaaacgacca cgagaagacg
     4261 ttccgtgcac cgaaagcgta tccggagcag tcgatggcgg ttgcggcgac acatgacctg
     4321 cctacgctac gcggttactg ggagagcggg gatctaacgc tgggcaaaac cctggggctg
     4381 tatccggatg aagtggtact gcgcggtctg tatcaggatc gcgaactggc gaagcaaggg
     4441 ctgctggatg cactgcataa atatggttgt ctgccaaaac gtgccgggca taaggcatcg
     4501 ttgatgtcga tgacgccgac gctgaaccgt ggtttgcagc gctacattgc cgacagtaac
     4561 agtgctctgt taggactaca gccggaagac tggctggata tggccgaacc ggtgaatatt
     4621 cctggcacca gttaccagta taaaaactgg cgacgcaagc tttccgcaac gcttgagtcg
     4681 atgtttgccg atgatggcgt gaacaagttg ctgaaggatt tggacagacg gcgcagagct
     4741 gcagcgaaga agaagtagag tgcggttgat gccggatgcg atgcatccgg cagtcagtgt
     4801 tatcaaatca ccatattcag cagcagacag cctaccagac cgcacaccga gataatggtt
     4861 tccagcatcg accaggattt gatggtctcg ccgatagtca ggttaaagta ctctttgaac
     4921 agccagaagc ccggatcgtt cacatgagag aaaatcacac taccggaacc caccgcaata
     4981 accatcagct ccgggctaac acccgtcgtt gcaatcagcg gtgccgcgat accacccgca
     5041 gtgattgccg caacggttgc ggaacccagc gcgatacgca gtacggcagc aatcgaccag
     5101 gccatcagca gcggagaaat gttggtttcg tgcatcatgg aagcaatgta tttgtccacg
     5161 ccgctgtcta ccagcacctg cttgaacgca ccgccaccac cgatgatcaa cagcatcatc
     5221 gcaatgattt tgatggaaga aaccagcgtg tcgttaatct gatccattga acgaccacgg
     5281 ttcagaccaa aggtgaacat ggcaatcagc acggcaatca gcgtagccat taccgggtca
     5341 ccgaggaact ccgctaccgg caggaaagcg tgacctttcg gcaggatcat ttcggcaatc
     5401 gcacgcatcg ccatcagtac taccggcacc agagaggtcc agacgctgac gccaaagctc
     5461 ggcatctctt cttcgctgaa ggttttcgcg ctgtagagac cttccggaat tggcttatcg
     5521 atacctttca acacgcgagc gtaaaccgga ccggcgagaa tcacggtcgg gattgccaga
     5581 atagtaccgt acagcagggt tttacccata tcggcattga aaatggtggc aatcgcagtc
     5641 ggacccggat gcggtggcag gaagccgtgg gtcacagaca gtgcagctgc cattggtaca
     5701 ccaacataca acagtggaat attcgcagaa gccgcgatgg taaacaccag cggcagcatc
     5761 agcacaaagc ccacttcata gaacagggca aaaccaacgg taaaaccggt cagtaccacc
     5821 gcccactgga tgtgtttttt accaaatttg gcaatcagcg tggtggcgat acgttgtgcg
     5881 ccaccgcagt ctgccagaat tttgcccagc attgcgccaa aacccatgat cagggcaagg
     5941 ctaccgagcg tcccgccgac accggctttg atggagccaa taactttatc cagcggcatt
     6001 ccttgcatta atccaacagc aagcgccacg aggacgagag cgatgaagcc gttcattttg
     6061 aagcggatca tcaggagcaa caacaagatt acaccgatag caacaatgac taatggcatg
     6121 atttacctgg cctttcattt gttatgggta acgtcaattt tctgacgaca aactctaatt
     6181 atcccaatcg ggaacagaga tattgcggca ccacgactga tacccactaa aactaattat
     6241 tgtagtcaga tgtcaggagt atgtttggta cccatgtgaa tgatacgggt aacatctggc
     6301 gtttgagaat caccagagcg gggtaaattt aaattatgag aggttggtca tattatcgcg
     6361 gggaaacgaa gggagggatt tgccaaagca atgctgtgcc aacgtctggc acatgttcaa
     6421 cgtaggcccg aaatgacgct ttagcgtcgc atcgggcaat ctacaaaaga ggggataact
     6481 tagtagtagg agtgttcgcc gcgctggtgt tcggtgagat cgcgcacacc tttcagctcc
     6541 gggaattcgt tcagcagctg cttctcgatc ccttctttca gcgtcacatc gaccatggaa
     6601 caaccgttac agccgccgcc aaattgcaga atggcgtaac cgtcttcggt gatttccatc
     6661 agcgaaacgc gaccaccgtg accagcaagc tgtgggttga tctgcgactg cagcatatac
     6721 tccacgcgct ccatcagcgg tgcatcgtct gccactttac gcattttggc gttcggggct
     6781 ttcagcgtta actgagaacc caactggtcg gtaacaaagt cgatctctgc atcttccagg
     6841 tatggtgcgc ttaactcatc aacatacgcg gtcagcaggt caaatttcag ggcagtgtcg
     6901 gtggcttcca cggcatccgg cggacaataa gaaacaccac attcagcgtt aggcgtgcca
     6961 gggttaatca caaatacgcg gatttgtgtc ccttcttcct gatttgccag cagtttggca
     7021 aagtgcgctt gtgcagcatc ggaaatacgg atcatagtaa tggcctaata gttgactatt
     7081 ttagttggtt ataatacgcc catcatcgag gctctacaag gttcgacaaa ggcaccagac
     7141 ctggacagtc gccgcaccat tgcgtaaaag caactgcgca atctctgcga cggtacttcc
     7201 ggtggtaacg acatcatcca caatcaccat atggcgacct tgcacgggca attcaagacg
     7261 aaaggcattt ttcaggttgc gcttgcgcag ccgggcactg agaaaatgct gggtcgcagt
     7321 ggcccgtgta cgtgtgacgg cttcgctatc ccattggcaa tgcaaccagc gtgataacgg
     7381 ctgacacagc aaatcgctct gattaaatcc ccgacgccag tgacgccgct gccataacgg
     7441 aacgctgatg atgcgatccg gcaattgcaa cccggtggtg cgacgagcgt gtaagacttc
     7501 caatagtaac agacgtgaca gggcgctggc gatttcactg cgccgggaaa atttaagctg
     7561 gtggataagc ggacttaacg gcggcgcata gtcggcaacc gtgaccagtc tttgccaggg
     7621 cggcggtttt tgcaggcagc gaccgcaggg aagatgggag tgtgtggcgg gtaatccaca
     7681 ttgtgggcat aacgttttat ctgtgcgggt ggcgcgtgaa cagaccgaac aaatccccca
     7741 atgacctaac gccagtggca ttcggcatag ccagcataat cccggtactg ttagcatatg
     7801 ttcatccttg taagtcaaaa gagaacaata gcggatgaat aacatctggt ggcagaccaa
     7861 aggtcagggg aatgttcatc ttgtgctgct gcacggatgg ggactgaatg ccgaagtgtg
     7921 gcgttgcatt gacgaggaac ttagctcgca ttttacgctg caccttgttg acctgcccgg
     7981 cttcgggcgt agccggggat ttggtgcgct gtcacttgct gatatggccg aagccgtgct
     8041 gcaacaggca cctgataaag ccatttggtt aggctggagt ctgggcgggc tggtggcaag
     8101 ccaaattgtt ttaacccatc ccgagcgtgt tcaggcgctg gtcaccgtgg cgtcgtcacc
     8161 ttgttttagt gctcgtgacg agtggccggg gataaaaccg gacgtactgg cgggatttca
     8221 gcagcaactc agtgatgatt ttcagcgtac agtggagcgg ttcctggcgt tacaaaccat
     8281 ggggactgaa acggcgcgcc aggatgcgcg ggcgttgaag aaaaccgttc tggcgttacc
     8341 gatgccggag gttgacgtgc ttaatggcgg gctggaaatc ctgaaaacgg tcgatctccg
     8401 tcagccgctg caaaacgtgt ccatgccgtt tttgcgattg tatggctatc tcgacggtct
     8461 ggtgccgcgc aaagtggtgc cgatgctgga taaactttgg cctcacagcg aatcatatat
     8521 cttcgccaaa gcggcccatg cgccgtttat ttcgcatccg gtcgagtttt gtcacctgct
     8581 ggtggcgttg aagcagaggg tgtaggcaac ttttgaaatg gcgagacaga aatttactgg
     8641 ctcgccgcag ccaactcttc ttctgacacc ccggtaaagc gcatgatgtc tgtaagaggg
     8701 actccggatt caagcattat tttggctata tgcagggctt tggattgttc cccctcctgc
     8761 cgcaatcttt ccgcaattgt cattaaactc tccttgtgtt tcggtgaacg ttcggcaacg
     8821 ccgtcgataa aatcgttaaa acgtaccgcg tcgccggttt gcagtatgta attaaacagc
     8881 cctttgattt gtctgtcatt agcgtatcca ctacttaata agcaggccat ttgctctacc
     8941 agccccatca gatcgcgttg acgaatatgt ttttgaatta actccagcag cgccatacgt
     9001 cggtgctgca tgatttcatc atcaggcatg acggtgacat caatcagcgg aaatgcggag
     9061 gcataaagct gccttgccag tttgggatcg gcgaaacaat ccagccaaca cagcgaatag
     9121 ggataggggc tttcaatacc gtggtaaaac aacaatggca ccaccatcgg caacgttttg
     9181 tatccagcat caaggtgatt ttgcattgcg gcaatagcgt aacgcatcat gcgaaatgcg
     9241 atcagtttgt ttgaggtgct ttgatgttca atcagacaat agatgtatcc tggtccttgt
     9301 tccgttttca ccgaccacag cacatcggaa tagctttcac gcagatcgtc atcaataaag
     9361 ctgctcgact ccagcttgag ggttttcata tcacaaagcg cgtgaatggg tgccggtaaa
     9421 tgaaacgcaa gaaaatcacg agccgtgtcc ggttggcgta aaaagagttt gaacagcgca
     9481 tcgtgtgggg tggaactctg ctttttgctc atggcgttcc ttcacctgtt gtttgaatgc
     9541 cgcgactcta cgcgccactc cacacctgtg tattagcgag ttttcatccc tgcgagatgc
     9601 tttcaaagga acttacgccg tgctgcaaga gttgcatttt tatggaaagc cgcacgcaaa
     9661 aaagcccgga tgcagacgca taccgggctt tccaaaacag gcgatggagt aaggttaacg
     9721 cagcgcccac cactcgcgca gacaggcttt tccttccggg cagcttttac aactgccaga
     9781 gaggcagccg tcaggttctt cctgaatccg cacggctttg cccatacttt ccagttgttg
     9841 cagcatggcg ttaatcattg gctgtggagt gttcaatgtc tggcttatct gggccgcttc
     9901 catacggccc cgtaacgcca gcaaatcgcg cacctgaata agtgaagcca ttaatggcaa
     9961 tcaccggtgg tgctggctgc gcagcaactg cttaccgact tgcgggttgc cagcagttcg
    10021 atatccaccc ggctacgcgc gcggcgcagc agaccgataa ccacgatgtt aaacaggata
    10081 accgccagaa tgcacaccag gctgtaagtt gggtgctgac tgtagctggc gacctgatag
    10141 aacaatgttg ccagcgagta agcgatattc agcccccaca ggatggagaa gcccatccag
    10201 ccacggcttg attcacgggc gatggctccc atcaccgaga tacatggtac atacagcagg
    10261 acgaaaatca ggtagctgta agctgctgct gcgctaccga atttctgatc catcacgccc
    10321 atcgccccgg tacccatttc gccgtcgcct ttgctggctt caatggggtt catcagtacg
    10381 ctaaggctga aggtgtcttt caggctctgc caggtttcat ctatcgcact gaacagctct
    10441 tcaccgaggt taaactctgc cggattgaac tcttcgtcct gaatattttc tgcggtgtag
    10501 agggtgttga gcgtacccac taccacttct ttcgccatgg cacctgtaaa caggccaacc
    10561 gttgcctgcc agttatcttc atgcacgcca attggcttga agaccggggt gatcacccgg
    10621 ctgacggacg ccagcgccga gtcgttgatg ttatcgacga ttttcccgct cagcgagaag
    10681 ctgttgaaag cgctcaggaa aatgctgacg atgatgatca ctttaccagc acgcagaacg
    10741 aagcctttca gacgctgcca ggtctggata atcaggcttt taacgtgtgg tacatgatag
    10801 accggcaact ccatgacaaa cggcgtcgct tcaccgcgca tgatggtgta cttgagcatc
    10861 aggccagtca gcaccgccat cacaataccc agcatataca gcgagaagac cgccagcgca
    10921 ccgttctgcc cgaagaaggc agccgcgaat actgcgaaga tagccagacg cgcgccgcag
    10981 gacataaacg gtgccatcat gatggtcatc agacgttcac gcggtgcatc aagcgtacgt
    11041 gcacccatta ccgacggtac gttacaaccg aaaccgacga tcagcggcac aaaggatttc
    11101 cccggcaagc ccagcgcctg catcagacgg tccatcacaa acgccgcacg cgccatatac
    11161 ccggagtcct caaggaagga gaggaacagg tacatcatgc caatctgcgg caccagtggc
    11221 agcacggtgt taatgccgcc acccaggccc tgggcgagga agatagtcag ccagtccggg
    11281 aagtggagcg tgtagccaat ccattgaata ccatgcacaa atagcgccac ggagccgacg
    11341 tcaaacagcg gctgtaacgc cccgccgatg ttgatagcca gcaggaacat caggtacatc
    11401 acaaagagga aaatcggcag accgaggaaa cggttgagca cgattttatc taccgcagtg
    11461 gtgaaacggc tgggttctgc cgtcagggtg ttgcttacca catcacagat ggcagcaatg
    11521 cactggtaac gcgcatcggc aatgtgcagc gccggatcgt ccatctcatt acgcagacgg
    11581 gcgagggcgg catccagatg ctgcgacgct tcaccggcgt aggcgcggct gtagatatcg
    11641 ccttccagca tttgcaggcc cagccagcga cgttgtttca gcgggatgtc ggaaggcatc
    11701 acttttgcca gtgaatctgc ttcgttgagt agcggctgtg cgtaatgcac cagttccaca
    11761 ttctcgttag ctttatagcg atcaatcgcc agcttgagcg cttcaatacc gcgaccacgg
    11821 gttgaaacca gcgggatcac tgggcagcca agacgcgccg acagagcatc aatttcaata
    11881 cgaatatttt gcttctcggc aatgtcgagc atgttcagtg ccacgatgca gggaatgccg
    11941 agttccagca gttgtagcgt caggtacagg ttacgctcaa ggttagacgc atccaccacg
    12001 ttaatcagca ggtcggcgtc gccactcaaa atgtagtgac aggcgatttg ctcatcgagc
    12061 gaggtctgcg atgagatggt ggtcagagaa taggtgccgg gcaggtccac cagcgtgacc
    12121 tgatgatcgg tggtggagaa ttgcccttct ttacgttcga cggtaacgcc agcccagtta
    12181 cctacacgct gacgtgcgcc agtgagctgg ttaaataagg ttgtcttgcc agaatttgga
    12241 ttaccaatta agcctatggt taattttttc attgttgtta tcaccgtatt aacaggaaac
    12301 cgcttccact tctaataagg ccagatcttt tttgcgtaat accaggctca cacgacgggt
    12361 ttcgatatga atggggtcgc cgagtggagc gacgcgcacc acattaaaag aggagccagg
    12421 taacatgcca agagaaagca gtttttggcg atatgccggg ctgatttcac gggaaaagcc
    12481 agtgattttc cacgcagtat ctggagtgta ttgcataggt gcctacttgt ttctcattaa
    12541 ctggataact acctcatggg gcgtttgaca gcgacatgag accagatgat ttgccaacga
    12601 aggagagatt aaataatcaa cagcacaatg ataatgagaa tggtttctat cagcaatact
    12661 taaaatgtgt gcgaatgttg gtttgaataa taatctgact caatgttgat gtggctcaag
    12721 gttatatcgc agcgaaaata taattcgaat agcgaattac caggactggc taatgtttaa
    12781 ttaaggtttc agctgttaat gaagtgataa atgttgccgg aatatattgt gagtttggct
    12841 gtggaataaa aatgccggag atattctccg gcacttttat gtcagacaag gcgttcgttg
    12901 ccagatgcgg cgtggacacc ttatcgcgca ggtttagggg tcaatcaggg cgtttaacgt
    12961 tttttgccca ttgccgccgc cagcgcatcc atcatcgcgc tattaccggc aggctgcgct
    13021 tcacgaccgc gtggtttggc tgccgggcgg ttgttttgcg ggcgttcatt accgccgccg
    13081 cgacgggcgt tggtttcgcc aggctgctcg tccagacgca tggtcagggc gatacgttta
    13141 cgctgaagat ccacttccag cactttcacc ttcacaatgt cgcccgcttt caccacggta
    13201 tgcggatctt ccacaaactt gttcgacaat gaagagatgt gaaccaggcc gtcctgatgc
    13261 acgccaatat cgacaaacgc gccaaagttg gtgacgttgg tgacagcgcc ttcgaggatc
    13321 atacccggtt gcaggtcgtt cattgtctcg acgccatcgg caaactgagc ggttttaaat
    13381 tccggacgcg gatcgcgacc cggtttttcc agctctttga tgatgtcagt tactgtcggc
    13441 acaccgaatt tttcatcagt aaagtcagac gctttcaggt tacgcagttc gctgctgtta
    13501 cccatcagat ctttcagtgc ctgctgtgtt gctgccagaa tgcgttccac caccggatag
    13561 gcttccgggt gaacggtaga ggcgtccagc gggttatcac cgtggttaat gcgcaggaag
    13621 cccgcgcact gctcgaaggc tttcggcccc agacggctga ctttcagcag ttgctgacgg
    13681 ttcttgaact ggccgttctc atcgcgccag gccacgatgt tttgcgccat catgcgcgtc
    13741 aggcccgcca cgcgggttaa tagcggaaca gaagcggtgt tgagatcgac gccaacggcg
    13801 tttacgcagt cttctactac tgcatccagt ttgcgggcca gttgcgtctg gctgacgtca
    13861 tgctgatact gacctacgcc gatagatttc ggatcgattt tcaccagctc cgccagcgga
    13921 tcctgcaaac ggcgggcgat agataccgca ccacgcagcg aaacgtcgag atccgggaac
    13981 tcctgcgctg ccagctcgga agccgagtaa accgacgcgc cagcttcgct gacgatcact
    14041 ttctgcgcgg tcactttcgg gaactgcttc tgcacgtcga gatagaaacg ttcggtttcg
    14101 cgggaagctg taccgttgcc gatcgccacc agttcaacgt tatgcttttc acacaaggca
    14161 gcaacggtca tcgctgcttt tgctgtttgt ccggtgtgcg ggtaaatggt atcggtcgcc
    14221 accagtttgc cagtggcatc gaccaccgct acttttaccc cggtacgcag acccggatcg
    14281 aggcccatcg tggcacgcag tccggcaggg gccgccatca gcagatcgtg caggttacgg
    14341 gcaaagacgt tgattgcttc atcttccgcg cgttcgcgca cggtgcccat cagttcggtt
    14401 tccagatgca tcagcacctt gatgcgccag gtccagctca ccacgccttt gcgccagcta
    14461 tccgccggag cattgttcag gcgcaggccg agatgatcca tgatgatttg ctcgcaatag
    14521 ctctcttttg gcggctcttc gaactgcgga tcggcattca gcgaaagctg gagtacgcct
    14581 tcgttacgcc cacggaacat cgccagcgcg cggtgagaag gcaccgtgga cagcggttcg
    14641 tgatgatcga aatagtcgcg gaattttgcc ccttcctctt ctttaccgct catcacggtt
    14701 gagaccagat gcgcgttctt ccacagataa tcacgcactt tcgccagcag cgcggcatct
    14761 tcggcaaacc gttccatcag gatatagcgc gcgccgtcca gcgcggcttt ggtatctgcc
    14821 acgcctttat ctgcatcaac atattgtgca gcggcgactt ctggcgtgtg tgacggatcg
    14881 ctccacagca ggtcagccaa cggctcaagc cctgcttcaa tggcgatttg cccgcgggtg
    14941 cggcgtttag gtttgtaggg caggtagaga tcttcgagtt cggttttgct tagtgtggcg
    15001 ttgatggcct tcgccagatc atcggtgagt ttgccttgct cggaaatgga tttgaggatc
    15061 gcctgacgtc tctcttccag ctcgcgcaga tagctcagac gcgtttccag attacgcagc
    15121 tgcgtgtcat ccagaccgcc ggtgatttcc ttacgataac gtgcgataaa cggcacggta
    15181 ttcccttcgt caagcaggcg aacggcagcg tcaacctgtt ccgggcgcgc ctgaatttca
    15241 cccgcaataa tgcggcagaa cgaatcattc atcatggttt gagttcatct tttcggatca
    15301 aaaatcaggg gatagttata cggactggct ggcaaaaatg ccagccatcg gcaggaggtt
    15361 aagactcttc cttacggttt cacgtactcg atagcattga cataccagct cgcttccccg
    15421 gcaggggtat tcaccaccgc cagatcgccg acttcttttt tcagcaatgc gcgggccatc
    15481 ggggaatcga tagagatgta atctttacgg ccaaaaattt catcgtagcc gacaatacgg
    15541 aaacggtgag tcacgccatc gtcgttttca atctccaccc atgcgccaaa aaagaccttg
    15601 ccttcctgct gaggggagta atcgacgatt ttgagatttt ccatgcattt agtgagatag
    15661 cgcacgcgac ggtcgatttc acgcagacgc tttttattat actgatagtc agcattttcg
    15721 ctgcggtcgc ccagacttgc ggcccaggtc accttttttg tgacctccgg gcgttcttca
    15781 cgccagagat aattaagctc ttgtttgagt ttttcatacc cttcccgggt aaccaggggc
    15841 gttttcatct cgttgattcc ctttgtctgt ttgataatgc gcacattggg tataacgtga
    15901 tcatatcaac agaatcaata atgtttcgcc gaataaattg tatacttaag ctgctgttta
    15961 atatgctttg taacaattta ggctgaaatt cataccagat ttagctggtg acgaacgtga
    16021 gcttttttaa gaatacacgc ttacaaattg ttgcgaacct ttgggagtac aaacaatgca
    16081 agagaactac aagattctgg tggtcgatga cgacatgcgc ctgcgtgcgc tgctggaacg
    16141 ttatctcacc gaacaaggct tccaggttcg aagcgtcgct aatgcagaac agatggatcg
    16201 cctgctgact cgtgaatctt tccatcttat ggtactggat ttaatgttac ctggtgaaga
    16261 tggcttgtcg atttgccgac gtcttcgtag tcagagcaac ccgatgccga tcattatggt
    16321 gacggcgaaa ggggaagaag tggaccgtat cgtaggcctg gagattggcg ctgacgacta
    16381 cattccaaaa ccgtttaacc cgcgtgaact gctggcccgt attcgtgcgg tgctgcgtcg
    16441 tcaggcgaac gaactgccag gcgcaccgtc acaggaagag gcggtaattg ctttcggtaa
    16501 gttcaaactt aacctcggta cgcgcgaaat gttccgcgaa gatgagccga tgccgctcac
    16561 cagcggtgag tttgcggtac tgaaggcact ggtcagccat ccgcgtgagc cgctctcccg
    16621 cgataagctg atgaaccttg cccgtggtcg tgaatattcc gcaatggaac gctccatcga
    16681 cgtgcagatt tcgcgtctgc gccgcatggt agaagaagat ccagcgcatc cgcgttacat
    16741 tcagaccgtt tggggcctgg gctacgtctt tgtaccggac ggctctaaag catgaggcga
    16801 ttgcgcttct cgccacgaag ttcatttgcc cgtacgttat tgctcatcgt caccttgctg
    16861 ttcgccagcc tggtgacgac ttatctggtg gtgctgaact tcgcgatttt gccgagcctc
    16921 cagcagttta ataaagtcct cgcgtacgaa gtgcgtatgt tgatgaccga caaactgcaa
    16981 ctggaggacg gcacgcagtt ggttgtgcct cccgctttcc gtcgggagat ctaccgtgag
    17041 ctggggatct ctctctactc caacgaggct gccgaagagg caggtctgcg ttgggcgcaa
    17101 cactatgaat tcttaagcca tcagatggca cagcaactgg gcggcccgac ggaagtgcgc
    17161 gttgaggtca acaaaagttc gcctgtcgtc tggctgaaaa cctggctgtc gcccaatatc
    17221 tgggtacgcg tgccgctgac cgaaattcat cagggcgatt tctctccgct gttccgctat
    17281 acgctggcga ttatgctatt ggcgataggc ggggcgtggc tgtttattcg tatccagaac
    17341 cgaccgttgg tcgatctcga acacgcagcc ttgcaggttg gtaaagggat tattccgccg
    17401 ccgctgcgtg agtatggcgc gtccgaggtg cgttccgtta cccgtgcctt taaccatatg
    17461 gcggctggtg ttaagcaact ggcggatgac cgcacgctgc tgatggcggg ggtaagtcac
    17521 gacttgcgca cgccgctgac gcgtattcgc ctggcgactg agatgatgag cgagcaggat
    17581 ggctatctgg cagaatcgat caataaagat atcgaagagt gcaacgccat cattgagcag
    17641 tttatcgact acctgcgcac cgggcaggag atgccgatgg aaatggcgga tcttaatgca
    17701 gtactcggtg aggtgattgc tgccgaaagt ggctatgagc gggaaattga aaccgcgctt
    17761 taccccggca gcattgacgt gaaaatgcac ccgctgtcga tcaaacgcgc ggtggcgaat
    17821 atggtggtca acgccgcccg ttatggcaat ggctggatca aagtcagcag cggaacggag
    17881 ccgaatcgcg cctggttcca ggtggaagat gacggtccgg gaattgcgcc ggaacaacgt
    17941 aagcacctgt tccagccgtt tgtccgcggc gacagtgcgc gcaccattag cggcacggga
    18001 ttagggctgg caattgtgca gcgtatcgtg gataaccata acgggatgct ggagcttggc
    18061 accagcgagc ggggcgggct ttccattcgc gcctggctgc cagtgccggt aacgcgggcg
    18121 cagggcacga caaaagaagg gtaaataaac gggaggcgaa ggtgcctccc gttttgcttt
    18181 ctataagata ctggatagat attctccagc ttcaaatcat tacagtttcg gaccagccgc
    18241 taccagcgcg gcacccgcag gggtgtcggt gtatttatcg aagttgtcga taaacagttt
    18301 cgccagggtt tcggcttttt cctgccactg ttccggagaa gcgtaggtgt tacgcggatc
    18361 gagaatcttc gtgtctacgc ccggcagttc ggttgggatc gccaggttaa acatcggcag
    18421 agtgaaggtt tctgcattat ccagcgaacc gttgaggatg gcgtcgataa tggcgcgggt
    18481 atctttaatc gagatacgtt tgccagtgcc gttccagcca gtgttaacca gataagcctg
    18541 cgcgcccgcc gcctgcatac gtttcaccag cacttctgcg tactgagtcg ggtgcagcga
    18601 caggaacgcc gcgccgaagc aagcggagaa ggttggcgtc ggttcggtga tgccacgctc
    18661 agtaccggcc agtttggcgg tgaagccaga gaggaagtga tactgggttt gatcggcagt
    18721 caggcgagaa accggcggca acacgccgaa agcatcagca gtcaggaaga taaccttagt
    18781 cgcgtggccc gctttggaaa ccggcttaac aatgttatcg atgtgataga tcggataaga
    18841 aacgcgggtg ttctcggttt ttgaaccatc atcaaagtcg atagtgccat cttcacgcac
    18901 ggtgacgttt tccagcagcg catcgcgacg gatagcgttg tagatttcag gttccgcttc
    18961 tttcgacagc ttgatagttt ttgcgtagca gccgccttcg aagttaaaca cgccgtcatc
    19021 gtcccagccg tgctcgtcat caccaatcag gcgacgtttc gggtcggtgg aaagggtggt
    19081 tttaccggtg ccggaaaggc cgaagaacac cgcaacatcg cctttctcac caacgttggc
    19141 ggagcagtgc atagaagcga tacctttcag cggcagcagg tagttcatca tcgagaacat
    19201 ccctttcttc atttcgccgc cgtaccaggt gccgccaatc agctgcatgc gctcggtcag
    19261 gttaaacgcc acgaagtttt cggagttcag accctgttct ttccactgcg ggttagtgca
    19321 cttcgcgccg ttcataacga taaagtctgg tttgaaacct gccagctctt catcgctcgg
    19381 gcgaataaac atgtttttga caaaatgcgc ctgccaggcc acttcggtga tgaaacggac
    19441 ggaaagacga gtatccgggt tcgcaccaca gaaagcgtcg acaacgaaca gacgtttgcc
    19501 ggaaagctgc ctggtcacca ggcctttcag atgctgccag gtttccggag agagaggttt
    19561 gttgtcgttc ttacctttgc ctttgtctgc ccaccagaaa gtatcgcgag tggtatcgtc
    19621 acggacgata tacttatctt ttggtgaacg accggtgaag atcccggtat cgacggcaac
    19681 ggcacccaga ttagttaaca ccccgcgctc ataacctgtc aggctcggat cgagctcttc
    19741 ctgatacagc aggtcgtagc ttgggttgta aacgatatca tgtacgtcac tgataccata
    19801 agcctcgagt tcttgcgggg tcaaaccatt gttaacgcgc atttcactgc tccttagcca
    19861 atatgtattg cctgaatagt aaagtctttt tgggggtgtt aaccgcgaca aggctcatag
    19921 atttacgtat ctggagaaat tcatatttga caaaaaactc cgtgattcct gtcacgaaac
    19981 ggttgctatt atcgcagaaa tagcatttta ggtgtggaaa atgttctcaa aaggttaaat
    20041 cttggtttat atggcgggaa tattgtgagt ggaatcgcat tccttaatga aattgatgca
    20101 aattcaatcg gtaaagtatc gattcaccac gctaactttt tttgattatt ttctgaaaaa
    20161 agcaaaatac tactgcccgg atgcgataat taacccacct tttgattctg gataaccaac
    20221 atggataacg ttgaactttc accggcaaca cgctggggga tgattgctac cggattactt
    20281 caggggctgg tttgctacct gttgatcgcc tggcttgccg gaaaaaatca cagttggatt
    20341 gtttatggcg tgcctgcgac cgttgcgttt tcatccgttt tactcttctc cgtgatctct
    20401 tttaaacaga agcgcctttg gggatggctg gcgctggtgt ttattgccac gacagggatg
    20461 agcggttggc tgaagtggca aactgatggc atgaacccct ggagagctga aaaggcgatt
    20521 tgggattttg gctgctatct gctgttgatg gcaatgttgt tgctaccgtg gatacaacaa
    20581 agccttcgta tccgcaatgg cagtagccgc tatagctact tttacaagtc agtatggcat
    20641 aacgtactta tattactggt gatttttctc gccaatggcc tgacgtgggt ggtgctttta
    20701 ctgtggggtg agttgtttaa acttgttggc atcaagtttt tcaatacgct cttttttgca
    20761 accgactggt ttatgtatct tacattaggt ctggttaccg cactggcggt gattctcgcg
    20821 cgcacacagt cacgtttaat cgactctatt caaaagttgt tcacgttaat cgccacgggg
    20881 ttgctgccgt tagtatcatt gctaaccctg atgtttatca tcactctgcc gtttacgggc
    20941 ctgagcgcga tttctcgcca catctccgcc gccgggttgc tgttaacgct ggcctttttg
    21001 caattgatct taatggctat tgtccgcgat ccgcaaaaag cgtcacttcc ctggacgggg
    21061 ccgttgcgtt gcctgattaa aaccgctttg ctggttgctc cgctgtatgt gttcgtcgcc
    21121 gcctgggcgt tatggctgcg ggtcgctcag tacggctgga ctgtcgaccg cttgcagggc
    21181 gcgctggcgg tgctggtgtt actggtgtgg tcgctggggt attttgtcag tatcgtctgg
    21241 cgtaatgggc aaaatcccct tgttcttcag ggcaaagtga accttgcggt ttcgttattg
    21301 gtgttggtga tactggtgct tcttaattcg ccggtgctgg atagtatgcg tattagcgtg
    21361 aacagtcata tggcgcgtta tcagagcggc aaaaacacgc cagaccaggt aactatctac
    21421 atgctcgaac agagcggtcg ctatggacgt gcggcgcttg agtcgctgaa aagcgatgcc
    21481 gggtttatga aagacccgaa acgcgcgcgg gatctgctga tggcgttaga tggagagcaa
    21541 catcttcagg aacaggtatc ggaaaaagta ttagccgaaa atgtgttaat tgcccctggt
    21601 tctgttaaac cggatgcgac attctggtcg gccttaatcc aggatcgcta taacgtgatg
    21661 acctgtattg aaaaagacgc ctgcgtcctg gtcgagcaag atctgaatag tgatggtcag
    21721 gcggagcgga tcctgtttgc ttttaatgat gacagagtca ttgtctatgg ctttgactca
    21781 gacagaaaag aatgggacgc gcttgatatg agtttacttc cgaacgaaat aacgaaagaa
    21841 aaattactca cagctgcgaa ggatggaaaa ctggggacga ggccgaaagc gtggcgcgat
    21901 ctgacggtag atggtgaaac gctggaaata aacttaagta agtgacaact cttttaacgg
    21961 atacgacgct gtatctgacc aacaataatt tgcgatttat ttcacaggcc tggtaagcgc
    22021 agcacatcag gccattacgt cgtaaaatcc ggcagagccc tctctgccgg acatactcat
    22081 taatgaactt gcggatctgc cggagacgcg ttgttgcgga tttcagcaat atccatcgca
    22141 ttgaacagat agtggttacc gcagtaatca caatgcatgt caatttcgcc atcttccgtc
    22201 aggatgctat caacttcttc atcaggcagc gttttcagcg catcggcgca acgttcacgc
    22261 gagcaggtgc atttgaactc cacatcctgc ggatcgtaaa ccgtcacctc ttcttcgtga
    22321 tacaaacgcc acaacacttc gtttgccggt aaggtcagca gttcttcggt tttgatggtt
    22381 tcggttagcg tcgccaggtg gtcaaagtcg tcctgctggg cattttgcgc aggcattacc
    22441 tgcaacaaca taccgcctgc agccggtttg ccgtctacgt cgccggtgcg aataaacagg
    22501 cgcgtcggca gctgttcaga acgcataaag taatcttcca ggcaggccgc cagggtatca
    22561 ccttccagac caaccacgcc ctgatagcgt tcgccttcgc tcggggtaat ggtgatcacc
    22621 acgtaaccat tgccgaccag cgttttcagg tcggcatttt ctggaatttc gccctgcacg
    22681 cgcgccacac cgcgcatttg ctggttattg ttaccgttaa taaccgccag attcatcgga
    22741 ccgtcgcctt gcagctgtac ggtgatatca ccatcaaact tcagcgtagc ggttaacagg
    22801 ctggtcgcaa ccagcagttc tgccagcacg tttttaacgg gctgcggata atcgtggttc
    22861 tcaaggatct gttgcagggt ttccgaaacg gttaccagtt cgccgcgaac ggcaaaattt
    22921 tcaaacagat agcgatgtaa ttggtcatgt tgcggcataa tcatctctct tgcaggtgac
    22981 agttattcac tgtcgccgtg tttaaatcgt aacaggtcgc ggcgctcttt tttgtccggg
    23041 cgtcggtccg ggtgcggcat ggttaaggca ttaagtttac gtgccatcgc cattttttcg
    23101 cgtttctcta cactttccgc agtctcttca tacagcaagg ctgcctcgct ggcggggcga
    23161 cgctgttcag taatcgcctt tacaatcacc gtgcgttcgt catttccctg gcgcagagtg
    23221 agcgtggtat tcagctcgac gattttgctc ggcttgctgc gctgcccgtt gtaatgcacc
    23281 ttaccgcctt caatcatttc acgggccagc gcgcgggttt tataaaaacg ggcagcccat
    23341 agccatttat ccagtcgaac ctcaacagca ggtttctctt tcatggcgtc tccttcacat
    23401 tagcgagggg atcaggcggc ggtagtcatt cagtgacgga tggcgttgat actgtttctc
    23461 ggcaatcccg gaatcaggat tagtcacgcc gaggcagtaa cgaataccaa attgcgcggc
    23521 agcatcgaga atcgcttcgc tgtcatcaat aaacagcgtt ctttcagctt tcagacccgt
    23581 agcttcggcc accgcatgcc ataaccgctg atcctctttc ggataaccaa atgtgtgggt
    23641 ggaaagtaat aaatcaaggt gtgcgtccag accggtatgc tcaagtttta ccgccaggtt
    23701 gtgcggatgc gcattggtga gcaaaattcg ctgcttaccg ctggctttca gtgcctcaag
    23761 aaacggaatg gtatcttcac gcagtacggc acgcggtccc atctcggtgg tcatcgcaca
    23821 gatatccaga cccagttgct cactccagta atcaagacag taccagttta gcgtatgctg
    23881 tacgtcgtga tattgctggc gcatatattc catcgcttcc tgtggcgtaa ccccgttttt
    23941 ggcgccccat gtttcaggca ccagcttttg ccagaaatag ttatcgaagg cgaggtcgag
    24001 caacgtgccg tccatatcca gcagaacggt atctacgtcc tgccaggcaa tgttgatatg
    24061 catgagggaa atctccagag tgaagcaatt tgcgcgacag ggtagcataa cctgccgcgc
    24121 aaacgtgtta ttcgataagg ctttctgaag gggtgatcag ttgcgggttc aggcagcttt
    24181 cataataggc ctgaatctcc atcatgcgag tgcgatgacg ctggtaacga cgccaggcct
    24241 gtacgccatt gtaaatggcg ctacccagca tcgtcagtag cagcaatgtg gtgctgagat
    24301 agcgccacag tccggaacga tccgggatcg gatgaaggcc aatatgctgc gtaccattgg
    24361 cgtcagtgaa gatttttgtg acgatacctt cggcgttaaa cggcgtatgc atcagcattt
    24421 gtgccagttt ctggaaagcg ttccactgtt cttgcggcgg gtagtcgtaa agtgatgccg
    24481 aaggccaggg ctgatcaaca aaatcgctgc cttcgtcgct gacaatcagg aatccgccgg
    24541 gggccggact gtttagtgat tgtgccgctc gcgccgtttc atgcgtgata aacggcgcgg
    24601 tggaggttgc caccaggtta tccagcgatt ctgcactcac cgggcgtaat aacacattca
    24661 cgccatcgag cttcccggcg ttggcgcgtt ttaccagcgc gtcccagtct ttactgttgc
    24721 cgagattgac cagcgcattt ttcagtcgca cacagtcatc tttggcagaa cataaatccg
    24781 ctgtcttcag tacaatgtcg ccaaaatcat caagcaatac catgccggat ttttgaatcg
    24841 ctgaacgtaa tgaggcactg acgcgagatt catcttccgg tttagggtgc agctggcgat
    24901 taactgcttc agtcaatgcc gtcgctttgt tgaccagttc agattctggt aatggtaatg
    24961 agcgggcgtc attccagatg atctgcgagc agtcaaacgg taaaaaaggt gaattggttt
    25021 tcgcgctcca ggttccggaa gtgcgaatat tacacattcc cgtaccgcta atacgcaatg
    25081 tatcgcctac ccgcacgcca gcgtcagcca gttgttttac gctggtggct tcaatggtct
    25141 gcgcgccttt catccatgag agagtgaatt tcagcggcat atccagcggg acccagaata
    25201 acagcataaa cagcaccagc agcgagcccg cagcgataat cgtactgcgc agccagtgct
    25261 gtaacggaaa gttttttact tcatcatgca gcgaaagata tcgcccctga cgcactacgt
    25321 ggcggtcgag atagatatca atatcggttt gttgaccgag atcctgagca atgtatggct
    25381 gccagtgtgc gggatagacc aggtcgataa taccgagtga aatattgttg atctgttcct
    25441 gatcgttttc gccaaacaat ccccaacgac ggggtgtacc gcgcagacaa tgaatttctc
    25501 gcagggagga ttttgccggg ggcgcgaata gcccccacag acctgcgccc agcagcagta
    25561 atgcgccgcc tgccagccac ggaacaaata catccggggt aatcaggcag aaaaagaaca
    25621 tcaggaagga ggcgacgatc agcaacgctt cacgcagccc gcgcggacga ctcagcgcat
    25681 attcttcatg cgtttctttg cgaatattga gtagctcgat ttgctcactc tcttccccgc
    25741 gaatcgacgc ctgcgtggac ggaacaggtt gcaaggcata gccgcgcgtt tcctgcatgt
    25801 actcctgcag cgtatgaccg ttgagtgaaa taaccagcgg cagcgaatcg gtataaatca
    25861 gttcaacggt attctcatcg ttgatgtatt gttcccagaa agggggcagg tggacttcta
    25921 ccgaatcgag gtagtaacgc catttattcg gatcgtcggt agagatgccg taacgcgtga
    25981 tagcgtgtgt cagcatcatg acgttgttgc tttcggcatt cagcgccaga gagatcggtg
    26041 ccgcgctggc tcccgttggg ccaggcacct gtaatacctg cgtcaggctc tcaagataat
    26101 tttcaacggc gctacgttct tcaggtgtga gtttacgcgt ttgcgcatcc gcgaaggcgt
    26161 tggtccaggg cagctgacgc cgtctggatc gcacttttat cagccatccc gcaagtagtg
    26221 agcaggccag caaagcagct aaaaaaatca caatggtgct catgctttcc ccatcttact
    26281 tagtctgtca ggcgtggtca gtcgcaccag acacaataca tgattctgcg gttggctatc
    26341 ggcaagcgtg gagtttaact tgagcatcat taagcacatc acagtaaccg ctgatcatag
    26401 caaaccctct gaaggcggaa tatcagcaaa ttcctggagt tatttcaacc tattgacttt
    26461 taatttgatt tgaggacgat tatttattaa gttacataaa tgtaaataac atgcaattca
    26521 cattgccgaa agcgtgtccg atatcgcaca ataaccgctg atttcacagc atagatccca
    26581 cgatgagcaa atcattacaa aaacccacca ttctgaatgt tgaaactgta gcccgttccc
    26641 gactgtttac cgtcgagagc gtggatctgg agttcagcaa tggcgtgcgg cgtgtttatg
    26701 aacgaatgcg tccaaccaac cgggaagcag tgatgattgt gccgattgtg gacgatcacc
    26761 tgatcctgat ccgcgaatac gcagtgggaa ctgaatccta cgaattaggt ttttcgaaag
    26821 gattaattga tccgggtgaa agcgtctacg aagccgctaa ccgcgagcta aaagaagagg
    26881 ttggatttgg tgcgaacgat ctgacttttt tgaagaagct cagcatggca ccgtcttact
    26941 tttccagcaa aatgaatatc gtggtcgcgc aagatctcta cccggaatcg ctggaaggcg
    27001 atgagccaga gccgctacca caggtgcgct ggccgctggc gcatatgatg gatttgctgg
    27061 aagaccctga cttcaacgaa gcgcgtaatg tcagcgcgct gttcctcgtg cgcgaatggt
    27121 tgaaagggca ggggcgagtg taaatgcgtg aagtttgcct gatgcacaac gcttatcagg
    27181 cttatgggtt tcctgcaata tattgatttt ttcacgattt tgttagccgg ataacgcgtt
    27241 cacccgcatc cggcataaac aaatgcactt tgtcagccaa ctgaaaaggc gccgaagcgc
    27301 ctttttaatc agaacaattc ctgtgcctca ccattatcga taatggtcgt tcccacctcg
    27361 tgcaccgcct gttgtgtcgg ctgcgtacct tcgatgaaat actcttcgcg gctgttgcca
    27421 ccattagcta actgcccggt gctgcgatcg atattcaccg tcacaatacc cggaggcggc
    27481 gtcagcggct gctccggcac accttcaaga acggctttca tataagcgtc ccatgcaggc
    27541 tgggcactct tggcaccgcc ttcgtaacct gagatctgat ctttaatcgc tccggaagcc
    27601 gttgtatgac cgagattacg acggtgatca tcaaagccaa tccagaccga ggtcacaacg
    27661 cccggaccgt aacccgagaa ccacgcatct ttcgaactgt tagtggtacc ggttttcccg
    27721 ccgatatcgc gacgctgcaa atcacgacct gcacgccagc cggtaccctg ccagcctggt
    27781 tcaccaaaga tattggtgtt caaagcactc ttaatcagga atgccagcgg agtgttgatg
    27841 acgtgcggtg cgtactcctg cgcgccagtc ttcgccacta acgcctgatt tgcctgctcc
    27901 agctgcggca ttggtacaga aacattctgc tgctcgcggg agatagcgac atcttcaaca
    27961 tcgttatttt ccagcacgtt cgatttctgc gtatcaccgt aaatcaccgg aatatcgcat
    28021 tccgggcagg ctactttcgg tttcgcttcg aaaatcacgc cgccctgatc gttttcaatt
    28081 ttgctgataa accacgggtc caccaggaag ccaccgttcg ccatgaccgc gtagccgcgc
    28141 gccacctgca ttggggtgaa ggacgctgaa cccagcgcca gcgattcggt gtggacaatg
    28201 ttttgcgccg ggaagccgaa gcgttgcaga tattctgcag cgtagtcgac gcccatcgcc
    28261 cgcattgcgc gtaccatcac cacgtttttc gactgaccca gcccctgacg taagcgaatt
    28321 ggaccagcat actgcggtgg tgagttcttc ggctgccagt cagaaccggc acctgcatcc
    28381 cagcgagaaa ttggcacatc gttcaacata cttgccagcg tcagaccttt atccatcgcc
    28441 gcggtgtaga ggaacggttt gatgttggaa cccacctgac gcagtgcctg ggtggcgcgg
    28501 ttaaacttgc tctgattgaa atcaaagcca ccgaccagcg ccataacggc accgttttgc
    28561 ggattgatcg acaccagcgc cgagttcact tccggcactt gtgccagcca ccatgcatcg
    28621 ccaacctgac gaacccagat ttgctgaccc gtttgcagaa catcggtcac tttacgcggc
    28681 gtcggtcctt gctgagtatc cgaacggtaa ggacgcgccc agcgaacgcc ttccatactc
    28741 aatgcgacgg tcgacccgtc cgccagcatc gccgtcgctt gctgaggatt ggcgctggtg
    28801 actgcggcag gcagcagcgg accataggtt ggcagcgcct tcagcgtatc ggtaatcttg
    28861 ttgttatccc acgccgactc gcccactttc cacagcacat ttgccgggcc gcgatagccg
    28921 tggcgcatgt cgtagtccag cacgttatta cgtaccgcct gctgtgcggc ctgctgcact
    28981 ttgcgggtga tggtggtgta aatgcgataa ccgtcttcat aggcactttc gccataacgg
    29041 ttatacatct cctggcgcac catttcgctc aggtacggcg cagagaaagc aatctccggc
    29101 gcgtgatagt tagcgttaat cgcctcagtg cgtgtctgat cgaactgttg ttgggtgata
    29161 tacccttcat ccagcatccg cgacagtacg acgttacgcc gcgcgacggc acgatccatt
    29221 gagtagagcg gattaaaggt ggaaggcgct ttcggcagcc cggcgatcac agccatttcg
    29281 ttcagcgtca gttggtcgac cgttttaccg aaatagactt gtgccgcagc accgacacca
    29341 taggcgcggt aaccgaggta aatcttgttc agataaagct cgaggatctc gtctttcgtc
    29401 agcaactgtt caatgcgaat cgcgaggaag acttccttaa tcttacgcat cagcgtgcgt
    29461 tctggactga ggaagaaatt tctcgccagc tgctgggtaa tggtactcgc cccttgtgac
    29521 gcgtggccgg agaacagcgc cacacttgcc gcacggaaga tccccaccgg gtcaacgccg
    29581 tgatgctcgt agaagcggct gtcttccgtc gcgataaagg ctttcaccat ctccggtggg
    29641 atttgatcca acgtaaccgg aatacgacgt ttctcaccgt attgagcaat cagctcgcca
    29701 tcggcgctgt aaatctgcat cggaatttgc aggcgaacat cttttaatgt cgccacatcc
    29761 ggcagttgtg gctcgatgta gcggtatagg ccataaatcg agcctgctcc cagcagaatg
    29821 caacagactg caaggatcaa aaaatacttt acgaacttca ctggaaattt cccatttagt
    29881 ttcatttggg cagtttataa acaaacgcgc ggtagtataa aggcaagcca gacgcattga
    29941 tatacccgtc agagtgacgg gtgataagga gagcatcaca atggcattta agatctggca
    30001 aattggtttg catttacaac agcaagaagc ggtagcggtt gcgatcgtgc ggggagcaaa
    30061 agaatgcttt ttgcaacgct ggtggcggtt gccgctggag aacgacatta tcaaagatgg
    30121 gcggattgtt gatgcgcagc agctggctaa aacgttgtta ccgtggagtc gcgaactgcc
    30181 gcagcgtcat cacattatgt tggcgtttcc cgccagtcgc acattacagc ggtcatttcc
    30241 gcgcccgtcg atgtcccttg gtgagcggga gcaaacggcc tggctgtcag ggacgatggc
    30301 ccgcgagctg gatatggatc cggactccct gcgtttcgat tacagcgaag actcactcag
    30361 tcccgcctat aacgtgactg ccgcgcaaag caaagagctg gcaacgctgc ttactctggc
    30421 agaaaggttg cgtgttcatg ttagtgcgat caccccggat gccagtgcat tacagcgatt
    30481 cctgcctttt ttaccttctc atcagcaatg tctggcctgg cgtgataacg aacagtggct
    30541 gtgggcgacg cgctatagct gggggcgcaa actggcggtg gggatgacta gcgcgaagga
    30601 gctggcggca gcgttatccg ttgatcccga cagcgtcgcg atatgtggcg aaggcggatt
    30661 tgacccctgg gaggccgttt ctgttcgtca gccgccgcta ccgccgcctg gtggagactt
    30721 tgccatcgcg ctggggctgg cgcttgggaa ggcgtactga tgaacccgcc aattaatttt
    30781 ttgccctggc gacagcaacg ccggaccgct tttctgcgtt tctggttgct gatgttcgtt
    30841 gcgcctctgc tgctggccgt cgggataacg ctaatactgc gtctgacagg cagcgccgaa
    30901 gcgcgcatag acgcagtttt gcttcaggcg gaacaacaac tcgcccgcag cttacagata
    30961 acgaagccac gtttgctgga gcaacagcag ttacgcgaac agcgttcgca acggcagcgc
    31021 cagcgacaat ttacccgcga ctggcaatct gcgctggaag cactggcggc gcttttaccc
    31081 gagcacgcct ggctgacaac gataagctgg cagcagggaa cgctggagat caaggggctg
    31141 acaacaagca ttaccgcgtt aaacgcacta gaaacgtcac tccgccagga tgcgtctttt
    31201 catctcaatc agcggggagc cacgcagcag gatgcgcagg gacgctggca atttgagtat
    31261 cagttaacaa ggaaggttag cgatgaacat gttctttgac tggtggttcg ccacatcacc
    31321 ccgcctccgc cagttttgct gggcattctg gttgctgatg ttagttacgc tcatttttct
    31381 gtcatcgaca caccatgaag agcgcgacgc attaattcga ctacgggcaa gtcatcacca
    31441 gcagtgggcc acactgtatc gcctggttga caccactccc ttcagcgaag aaaaaacgct
    31501 gcccttttcg ccactggatt ttcagttgcc cggcgcgcaa ctggtttcct ggcatccatc
    31561 cgcgcaggga ggtgagttgg cgttgaaaac gctgtgggaa gcagtgccgt cggcatttac
    31621 acggctggca gagcgcaacg tcagcgtgag ccgtttttcg ttaagcgtgg aaggtgatga
    31681 tcttttgttc acgctacaac tggagacacc gcatgagggt taaacgctgg ttgttggcag
    31741 gtattgcatt gtgcctttta accggtatgc gtgacccttt taaaccgccg gaagatctat
    31801 gccggattag cgaacttagc cagtggcgct atcaggggat ggtagggcga ggcgagcaca
    31861 tcatcggtgt aataaaagac gggcaaaaga aatggcgacg ggtgcagcaa aacgatgtgc
    31921 tggaaaacgg ctggacaatt ttacagctga cgccagacgc actaacgctg ggaaccggga
    31981 caaactgcga accgccacaa tggttgtggc aacggcaagg agatacaaat gaagcaatgg
    32041 atagccgcac tactgttgat gctgataccc ggcgtacagg cggcaaagcc gcaaaaagtg
    32101 acgctgatgg tggatgacgt tccggtagct caggtgttgc aggcgctggc tgaacaggag
    32161 aagttgaacc tggtcgtgtc gccagacgtc agcggtacgg tgtcgttaca tctaacagat
    32221 gttccctgga agcaggcact acaaactgta gtgaaaagcg ccggactgat aacgcggcag
    32281 gaagacaaca ttctctcagt gcattccatt gcctggcaga ataacaatat cgcccgccag
    32341 gaggcggagc aggcgcgggc gcaggcaaat ctgccgctgg aaaatcgcag tataaccctg
    32401 caatacgccg acgcgggaga actggcgaaa gcgggggaga agctactgag tgccaaaggg
    32461 agtatgaccg tcgataaacg caccaatcgc cttttgctac gagataacaa aacggcgtta
    32521 agcgcgcttg aacagtgggt agcgcaaatg gatctgccgg tcgggcaggt tgagctgtcg
    32581 gcgcatattg tcaccattaa tgaaaaaagt ttgcgtgagt taggcgtgaa atggacgctg
    32641 gccgatgcgc aacacgctgg tggcgttggg caagtcacca cgcttggtag cgacctctcc
    32701 gtagcgacgg cgacaacgca tgtcggtttt aacattgggc gcatcaacgg acgcttgctg
    32761 gatcttgagc tttctgcgct cgaacaaaaa cagcagctgg atattatcgc cagtccgcgt
    32821 ctgctggcct cacatcttca gcctgccagc attaaacagg ggagcgaaat tccatatcag
    32881 gtttccagcg gggaaagtgg cgcgacgtcg gtggaattta aagaggccgt cctggggatg
    32941 gaggtcacgc ccacggtgtt acaaaaaggt cgcatccggc tgaaattaca catcagccag
    33001 aacgttccgg ggcaggtgct acagcaggcc gatggcgaag tgctggcgat tgataagcag
    33061 gagatcgaaa cgcaggtcga ggtcaaaagc ggagaaacgt tggcgctggg cggcattttt
    33121 acccgtaaaa ataaatcggg tcaggatagc gtaccgttgc ttggcgacat tccctggttc
    33181 gggcaattat ttcgtcatga cggaaaagaa gatgaacgac gcgagttagt ggtgtttatc
    33241 acgccacgac tggtttccag tgagtaaaca gccgtaaaag cggtaatgtt tttatgctga
    33301 acgtgtttca tctatttgac gcgcgcaggt atttagcata caaggagtac cgatttgaga
    33361 gttggtgctc ttcgctgcct gcgttccatg atgatgattt atcattcagg cggcattttg
    33421 ctgtcttttt tacgctaatc ttacccggtg atttatcgcc agagcggtgg tagcaaggca
    33481 gcgcgcttgc agcgaccaga tatgcagagg gatgggtgat ttattcagtt gccaaacccg
    33541 ctggagtatt gagataattt tcagtctgac tctcgcaata tcttatgagg tttcagttca
    33601 tgtcctgcgg cgctctctga gcgaagcggg tttatcatta acgaatagtc ttagtagtac
    33661 cgaaaaaatg gcagagaaac gcaatatctt tctggttggg cctatgggtg ccggaaaaag
    33721 cactattggg cgccagttag ctcaacaact caatatggaa ttttacgatt ccgatcaaga
    33781 gattgagaaa cgaaccggag ctgatgtggg ctgggttttc gatttagaag gcgaagaagg
    33841 cttccgcgat cgcgaagaaa aggtcatcaa tgagttgacc gagaaacagg gtattgtgct
    33901 ggctactggc ggcggctctg tgaaatcccg tgaaacgcgt aaccgtcttt ccgctcgtgg
    33961 cgttgtcgtt tatcttgaaa cgaccatcga aaagcaactt gcacgcacgc agcgtgataa
    34021 aaaacgcccg ttgctgcacg ttgaaacacc gccgcgtgaa gttctggaag cgttggccaa
    34081 tgaacgcaat ccgctgtatg aagagattgc cgacgtgacc attcgtactg atgatcaaag
    34141 cgctaaagtg gttgcaaacc agattattca catgctggaa agcaactaat tctggcttta
    34201 tatacactcg tctgcgggta cagtaattaa ggtggatgtc gcgttatgga gaggattgtc
    34261 gttactctcg gggaacgtag ttacccaatt accatcgcat ctggtttgtt taatgaacca
    34321 gcttcattct taccgctgaa atcgggcgag caggtcatgt tggtcaccaa cgaaaccctg
    34381 gctcctctgt atctcgataa ggtccgcggc gtacttgaac aggcgggtgt taacgtcgat
    34441 agcgttatcc tccctgacgg cgagcagtat aaaagcctgg ctgtactcga taccgtcttt
    34501 acggcgttgt tacaaaagcc gcatggtcgc gatactacgc tggtggcgct tggcggcggc
    34561 gtagtgggcg atctgaccgg cttcgcggcg gcgagttatc agcgcggtgt tcgtttcatt
    34621 caagtcccga cgacgttact gtcgcaggtc gattcctccg ttggcggcaa aactgcggtc
    34681 aaccatcccc tcggtaaaaa catgattggc gcgttctacc agcctgcttc agtggtggtg
    34741 gatctcgact gtctgaaaac gcttcccccg cgtgagttag cgtcggggct ggcagaagtc
    34801 atcaaatacg gcattattct tgacggtgcg ttttttaact ggctggaaga gaatctggat
    34861 gcgttgttgc gtctggacgg tccggcaatg gcgtactgta ttcgccgttg ttgtgaactg
    34921 aaggcagaag ttgtcgccgc cgacgagcgc gaaaccgggt tacgtgcttt actgaatctg
    34981 ggacacacct ttggtcatgc cattgaagct gaaatggggt atggcaattg gttacatggt
    35041 gaagcggtcg ctgcgggtat ggtgatggcg gcgcggacgt cggaacgtct cgggcagttt
    35101 agttctgccg aaacgcagcg tattataacc ctgctcaagc gggctgggtt accggtcaat
    35161 gggccgcgcg aaatgtccgc gcaggcgtat ttaccgcata tgctgcgtga caagaaagtc
    35221 cttgcgggag agatacgctt aattcttccg ttggcaattg gtaagagtga agttcgcagc
    35281 ggcgtttcgc acgagcttgt tcttaacgcc attgccgata gtcaatcagc gtaacaacaa
    35341 gaaaggtcag gccgcttatc aagcggtcta ttagcttcag gttaattgca atgtggtaag
    35401 cattaacctt ttagtggggt gttaaatgga tgaattcaaa ccagaagacg agctgaaacc
    35461 cgatcccagc gatcgtcgta ctggtcgttc tcgtcaatct tctgaacgtt ctgagcgtac
    35521 tgaacgtggc gaaccgcaga tcaattttga tgatattgaa cttgatgaca ctgacgatcg
    35581 ccgtccgact cgtgcgcaaa aagagcgcaa tgaggaaccg gaaatcgaag aagaaattga
    35641 cgaatccgaa gatgaaaccg tggatgaaga gcgcgtagag cgtcgtccgc gtaagcgcaa
    35701 aaaagcagcc agtaaacccg cttctcgtca gtatatgatg atgggcgtcg gcattctggt
    35761 tctactgctg ttgatcatcg gtatcggttc tgcgctaaaa gccccctcga cctcttccag
    35821 cgatcaaacc gcgtctggcg agaagagtat tgatcttgca ggcaatgcga ccgatcaggc
    35881 gaatggggtg cagccagcgc cgggaaccac gtctgcggaa aatactcagc aggatgtttc
    35941 tctgccaccg atctcttcta cgccgactca agggcaaacc ccggcggcaa cggatggtca
    36001 acaacgtgtt gaagtgcagg gtgacctgaa caatgcgctg acccagccac aaaatcagca
    36061 acagttgaac aatgtggcgg tcaattctac attgccgact gaaccagcaa ctgtcgcgcc
    36121 agtacgcaat ggcaatgcat cgcgtgacac ggcgaaaacg caaactgctg aacgtccggc
    36181 cactacgcgt ccagctcgtc agcaggcggt gattgaaccg aaaaaaccgc aagcaaccgt
    36241 gaaaacggaa ccgaagccgg tagcacagac gccgaagcgt actgaaccag ctgctcctgt
    36301 ggcgagcacg aaggcaccgg ctgcgacttc tgcgccagca ccaaaagaga cggcgactac
    36361 ggctccagta cagacggcat ccccggcgca aaccacggca acaccagccg ctggagggaa
    36421 gaccgcaggt aatgttggtt cgttgaaatc ggcaccgtcc agccattaca ctctgcagct
    36481 gagcagttcc tctaactacg acaacctgaa cggttgggcg aagaaagaga atctgaaaaa
    36541 ctacgttgtc tatgaaacga cgcgtaatgg tcagccgtgg tatgtcctgg tttctggcgt
    36601 gtacgcttcg aaagaagagg cgaaaaaagc ggtatctaca ttgccagcag atgttcaggc
    36661 caaaaacccg tgggcgaaac cgctgcgtca ggtacaggcc gatctgaagt aatcaaggtt
    36721 atctcccgca atgggttatc gttgcgggag ttgcctgaag cgctggatgc tgtcggagct
    36781 ttctccacag ccggagaagg tgtaattagt tagtcagcat gaagaaaaat cgcgcttttt
    36841 tgaagtgggc agggggcaag tatcccctgc ttgatgatat taaacggcat ttgcccaagg
    36901 gcgaatgtct ggttgagcct tttgtaggtg ccgggtcggt gtttctcaac accgactttt
    36961 ctcgttatat ccttgccgat atcaatagcg acctgatcag tctctataac attgtgaaga
    37021 tgcgtactga tgagtacgta caggccgcac gcgagctgtt tgttcccgaa acaaattgcg
    37081 ccgaggtcta ctatcagttc cgcgaagagt tcaacaaaag ccaggatccg ttccgtcggg
    37141 cggtactgtt tttatatttg aaccgctacg gttacaacgg cctgtgtcgt tacaatctgc
    37201 gcggtgagtt taacgtgccg tttggccgct acaaaaaacc ctatttcccg gaagcagagt
    37261 tgtatcactt cgctgaaaaa gcgcagaatg cctttttcta ttgtgagtct tacgccgata
    37321 gcatggcgcg cgcagatgat gcatccgtcg tctattgcga tccgccttat gcaccgctgt
    37381 ctgcgaccgc caactttacg gcgtatcaca caaacagttt tacgcttgaa caacaagcgc
    37441 atctggcgga gatcgccgaa ggtctggttg agcgccatat tccagtgctg atctccaatc
    37501 acgatacgat gttaacgcgt gagtggtatc agcgcgcaaa attgcatgtc gtcaaagttc
    37561 gacgcagtat aagcagcaac ggcggcacac gtaaaaaggt ggacgaactg ctggctttgt
    37621 acaaaccagg agtcgtttca cccgcgaaaa aataattctc aaggagaagc ggatgaaaca
    37681 gtatttgatt gccccctcaa ttctgtcggc tgattttgcc cgcctgggtg aagataccgc
    37741 aaaagccctg gcagctggcg ctgatgtcgt gcattttgac gtcatggata accactatgt
    37801 tcccaatctg acgattgggc caatggtgct gaaatccttg cgtaactacg gcattaccgc
    37861 ccctatcgac gtacacctga tggtgaaacc tgtcgatcgc attgtgcctg atttcgctgc
    37921 cgctggtgcc agcatcatta cctttcatcc agaagcctcc gagcatgttg accgcacgct
    37981 gcaactgatt aaagaaaatg gctgtaaagc gggactggta tttaacccgg cgacaccgct
    38041 gagctatctg gattacgtga tggataagct ggatgtgatc ctgctgatgt ccgtcaatcc
    38101 tggtttcggc ggtcagtcgt tcattcctca aacactggat aaactgcgcg aagtacgtcg
    38161 tcgtatcgac gagtctggct ttgacattcg tctggaagta gacggtggcg tgaaggtgaa
    38221 caacattggc gaaatcgctg cggcgggcgc ggatatgttc gtcgccggtt cggcaatctt
    38281 cgaccagcca gactacaaaa aagtcattga tgaaatgcgc agtgaactgg caaaggtaag
    38341 tcatgaataa gtttgaagat attcgcggcg tcgcttttga tcttgatggt acgctggtcg
    38401 acagtgctcc cggtcttgct gctgcggtag atatggcgct gtatgcgctg gagttgcccg
    38461 tcgcaggtga agaacgcgtt attacctgga ttggtaacgg cgcagatgtt ctgatggagc
    38521 gcgcattgac ctgggcgcgt caggaacgtg cgactcagcg taaaacaatg ggtaaaccgc
    38581 ccgttgatga cgacattccg gcagaagaac aggtacgtat tctgcgtaaa ctgttcgatc
    38641 gctactatgg cgaggttgcc gaagagggga cgtttttgtt cccgcacgtt gccgatacgt
    38701 tgggcgcgtt gcaggctaaa ggcctgccgc taggcctggt caccaacaaa ccgacgccgt
    38761 tcgtcgcgcc gctgctcgaa gccttagata ttgccaaata ctttagcgtg gttatcggcg
    38821 gcgatgatgt gcaaaacaaa aaaccgcatc cggacccgct gttactggtg gctgagcgga
    38881 tgggaattgc cccacaacag atgctgtttg tcggcgactc acgcaatgat attcaggcgg
    38941 caaaagcggc aggttgccca tcagttggct taacctacgg atataactac ggcgaggcta
    39001 tcgatctcag ccagcctgat gtaatttatc agtctataaa tgaccttctg cccgcattag
    39061 ggcttccgca tagcgaaaat caggaatcga aaaatgacta agcccatcgt ttttagtggc
    39121 gcacagccct caggtgaatt gaccattggt aactacatgg gtgcgctgcg tcagtgggta
    39181 aacatgcagg atgactacca ttgcatttac tgtatcgttg accaacacgc gatcaccgtg
    39241 cgccaggatg cacagaagct gcgtaaagcg acgttggata cgctggcctt gtatctggct
    39301 tgtggtatcg atcctgagaa aagcaccatt tttgttcagt cccacgtacc agaacatgcg
    39361 cagttaggct gggcactgaa ctgctatacc tacttcggcg aactgagccg catgacgcag
    39421 tttaaagata aatctgcgcg ttatgccgag aacatcaacg ctggtctgtt tgactatccg
    39481 gtgctgatgg ctgcggacat cctgctgtat caaactaatc tggtaccggt gggtgaagac
    39541 cagaaacagc acctggaact gagtcgcgat atcgcccagc gtttcaacgc gctgtatggc
    39601 gagatcttta aggtgccgga gccgtttatt ccgaaatctg gcgcgcgcgt aatgtcgctg
    39661 ctggagccga ccaagaagat gtccaagtct gacgataacc gcaataacgt tatcggcctg
    39721 ctggaagatc cgaaatcggt agtgaagaaa atcaaacgcg cggtcactga ctccgacgag
    39781 ccgccggtag ttcgctacga tgtgcagaac aaagcgggcg tttccaacct gctggatatc
    39841 ctttctgcgg taacgggcca gagcattcca gaactggaaa aacagttcga aggcaagatg
    39901 tatggtcatc tgaaaggtga agtggctgat gccgtttccg gtatgctgac tgaattgcag
    39961 gaacgctatc accgtttccg caacgatgaa gccttcctgc aacaggtgat gaaagacggc
    40021 gcagaaaaag ccagtgtaca cgcttcccgt acgctgaaag cggtgtacga agcgattggt
    40081 tttgtggcga agccgtaata tcaaaacgct cggacggtcc cctcgcccct ttggggagag
    40141 ggttagggtg aggggagcag gcaagcgcca gtgggaatta ccctttgctc gcattgtcga
    40201 ataaatcact tcgtcatcac tctgaaaccg gggaaacccg gtttttttat cctcatttgc
    40261 aataatccga aaaaatgtga agcgcctcgc cgtttccaca tctcacagtt gccacttatc
    40321 ttcattttaa tgaagatata aacattcatt ttattgaaaa tatattatgc gtcgaacgtt
    40381 tatcaaaaaa gaaggcgtcg ttatcacgac gctggcccgt tatttgttgg gtgaaaaatg
    40441 cggtaatagg ttgaaaacca tagatgagct ggcaactgaa tgccgttcat ccgttggcct
    40501 gacgcaggcc gcgttgaaaa cgctggaatc aagcggagcg atacggattg aacgccgtgg
    40561 gcgcaatggc agttatctgg tcgagatgga taacaaagca ttgctgagtc atgtggatat
    40621 caacaacgtg gtatgtgcga tgcctttgcc ctatacccgc ttgtacgaag gtttagcgag
    40681 cggattgaaa gcccagtttg atggcattcc tttttactat gcgcatatgc gcggcgcgga
    40741 tattcgcgtg gagtgtttac ttaacggcgt gtatgacatg gcggtggttt cccgactggc
    40801 ggcggaaagt tatctcacgc aaaaagggtt ttgcctcgcg ctggagttgg ggccgcacac
    40861 ctacgttggc gagcaccagt tgatttgccg taaaggcgag tccgcaaacg tgaagcgcgt
    40921 ggggctggat aaccgttcgg cggatcagaa aatcatgacc gatgtctttt ttggcgatag
    40981 tgatgtggaa cgtgtcgatc tctcttatca cgagagttta caacgcattg ttaaaggcga
    41041 tgtcgatgcg gtgatctgga acgtggtggc ggaaaacgaa ctgaccatgc tgggattaga
    41101 ggcgacgccg ctcacagacg atccgcgatt tttacaggcc accgaagccg tggttctgac
    41161 gcgagtcgat gattacccaa tgcaacaact gctgcgtgcc gttgtagata aacacgccct
    41221 gctcgcccat caacaacggg tagtgagtgg ggaacaggaa ccgagttatt aaacgaaagg
    41281 catctgatat ggaaaccaga ctcaacctgc tttgcgaggc aggcgttatt gataaggaca
    41341 tctgcaaagg catgatgcag gtcgtcaacg tactggaaac agagtgccat ctgccggtgc
    41401 gcagtgagca aggaacgatg gcgatgacac atatggcgag tgcactgatg cgcagtcgcc
    41461 gtggtgaaga aatagagccg ctggataacg aattgctggc agagctggcg caatccagcc
    41521 actggcaagc cgttgtgcaa ttgcatcagg tgttgttgaa ggaattcgca ctggaagtta
    41581 acccgtgtga agaaggctat ttgctggcca acctttatgg attatggatg gctgctaacg
    41641 aagaggtttg aagctcccga caaatccacg tcgcacaacc taaatattca aaaaaatgaa
    41701 tatttaattc aaggaacaga aacgaggcaa caagatgttt gtagaagcat tgaaacgcca
    41761 gaacccggcg ctgatttccg ccgtactaag tttgtggcag cagggcaaga tcgccccgga
    41821 cagctgggtg atcgacgtgg atcaggtact ggaaaacggt aagcgactga ttgagacggc
    41881 gcggctttac ggcattgaac tgtatctgat gaccaagcag tttggccgta acccatggct
    41941 ggcggaaaaa ttgctggcat taggttacag cggcattgtg gcggtggatt acaaagaggc
    42001 gcgagtcatg cgccgcgctg gtttgtcggt ggcgcatcag gggcatctgg tacaaatccc
    42061 ttgtcatcag gttgctgacg ccgttgaaca gggcaccgac gtcatcaccg tgtttactct
    42121 cgacaaagcg cgggaagttt ctgcggcagc ggtgaaggcc ggacgtgtgc agtttgtgct
    42181 gctcaaagtt tatagcgacg atgattttct ttatccgggg caggagagcg gttttgtaca
    42241 gcattcgctg cacgaggtgg ttgctgaaat taagaaactg ccgggtctgc atttagccgg
    42301 acttacccat ttcccttgcc tgctttggga tgaggctgcc gggaaagttt tgccgacacc
    42361 gaatcttcac acgctggtac aagcacggga tcaactggcg aaatctggta ttgcgattga
    42421 gcaactgaac gcgccttctg cgaccagctg cacttcgctg ccgatgctgg cggaatacgg
    42481 tgtgactcat gccgaacccg gtcatgcgct gacgggcact attccggcaa accagcaggg
    42541 cgatcagcct gaatatatag cgatgctttg gttaagtgaa atctcccatc atttccgtgg
    42601 cgacagctac tgctacggcg gcggttacta ccgtcgtggt catgcgcaac atgcgctggt
    42661 gtttacgcca gaaaatcaaa agattactga aaccaatctc aaaactgtgg atgacagcag
    42721 tatcgactac accctgccgc tggcaggcga gtttccggta agcagtgcag tagtgctctg
    42781 ttttcgcacg cagatttttg tcactcgtag cgatgtggtg ctggtgtccg gtattcaccg
    42841 tggcgaaccg aaaatcgtcg gtcgttatga cagtcttgga aactctctgg gggcgtaatg
    42901 gcgcgatttg tggtgttagt gattgatagc tttggcgtag gggcaatgaa agatgtcacg
    42961 ctggtgcgcc cgcaagatgc cggggcgaat acctgtggtc acatcctgag ccagttgccg
    43021 catttgcagc taccagcgct ggagaagctg gggctaatca acgcattggg ttatgcacca
    43081 ggtgatatgc aaccgtcaga ttccgcaacc tggggggtgg cagagctgca acatgaaggt
    43141 ggcgatacct ttatggggca tcaggaaatt ttaggcacgc gcccgttacc gccgctgcgg
    43201 atgccctttc gcgatgtgat tggccgtgtt gagcaggcat tagtttccgc tggctggcag
    43261 gtggagcgcc gtggcgatga tctgcaattt ctgtgggtca atcaggcggt tgcgattggc
    43321 gataatctcg aggcggattt aggccaggtc tataacatta ccgccaatct ctctgtgatc
    43381 tcttttgacg acgcgatcaa aattggtcgt atcgtgcgtg agcaggtaca ggtcggtcgg
    43441 gtcattacat ttggcggctt gttaaccgac agtcagcgta ttctcgatgc cgcagaaagc
    43501 aaagaagggc gctttattgg tattaatgcg ccgcgttctg gcgcttatga caacggcttc
    43561 caggtcgtgc atatgggcta cggtgttgat gaaaaagtgc aggtgccgca aaaactgtat
    43621 gaggcgggcg tgccaaccgt gctggtaggt aaggtggcag atatcgtcag caatccttat
    43681 ggcgtgagct ggcaaaacct ggtggatagc cagcggatta tggatatcac cctcgacgaa
    43741 tttaacacgc atccgacggc gtttatttgc atcaatatcc aggaaaccga cctcgctggt
    43801 catgcagaag acgtcgcacg ttatgccgaa cgtttgcagg tcgttgaccg taaccttgcc
    43861 cggcttgttg aggcgatgca gccagatgat tgcctggtcg tgatggcgga tcacggtaac
    43921 gatccgacca ttggtcacag ccaccatacc cgtgaagtgg tgccagtgct ggtttatcag
    43981 caagggctgg tccacacgca actcggtgta cgcaccacgc tttctgatgt gggggctacc
    44041 gtgtgtgaat ttttccgtgc gccaccgcca caaaatggtc gctcttttct ttcctccctc
    44101 cggtttgcag gagacaccct atgagtattg atccgacggg ttacaccctg gcccatgagc
    44161 atctgcatat tgatctctcc ggctttaaaa acaacgtgga ctgccgcctt gatcagtatg
    44221 cgttcatttg ccaggagatg aacgacctga tgacccgggg cgtgcgtaat gtgattgaga
    44281 tgaccaaccg ttacatgggg cgcaatgcgc aatttatgct cgatgtaatg cgcgagacgg
    44341 ggatcaacgt ggtggcctgc accggttatt accaggacgc ttttttcccg gagcatgtgg
    44401 cgacccgcag cgtgcaggaa ctggcgcagg agatggtcga tgaaattgaa cagggtatcg
    44461 atggcaccga cctgaaagcc gggattatcg cggagatcgg ctctagcgaa gggaagatta
    44521 cgccgctgga agagaaggtg tttattgccg ctgcgctggc gcataaccag accggacgcc
    44581 cgatctccac gcatacttcg ttcagcacca tggggctgga acaactggct ctgttgcaag
    44641 cccacggagt tgatctttcg cgcgtcaccg ttggtcactg cgatctgaaa gacaatctcg
    44701 acaacatttt gaagatgatc gatctcggcg cgtacgtgca gttcgacacc attggtaaga
    44761 acagttacta cccggacgaa aagcgcattg cgatgctcca cgcgctacgt gaccgtgggt
    44821 tgctgaaccg cgtcatgctg tcgatggata ttacgcgccg ctcccattta aaagccaatg
    44881 gtggttatgg ctatgactat ttattgacca cctttattcc gcaattgcgc cagtcaggat
    44941 tcagtcaggc cgatgtggat gtgatgttac gtgaaaatcc ctctcaattt ttccaataag
    45001 gacagactca tgaaaaagat tggcgttgca ggcttacagc gtgagcagat taaaaaaacc
    45061 attgaagcga cggctcctgg cagttttgaa gttttcattc acaacgacat ggaagcggca
    45121 atgaaagtga aatccgggca actggattac tacatcggtg cgtgtaatac cggtgcgggc
    45181 gcggcattgt cgattgccat cgcggtgatt ggctataaca aaagttgcac cattgccaaa
    45241 ccaggcatta aagcgaaaga cgagcatatc gccaaaatga tcgctgaagg gaaagtggcg
    45301 tttggccttt ccgttgagca cgtcgaacac gcgattccga tgctgattaa ccatctgaaa
    45361 taaaaggcac gactatggat ctgtatattc agattatcgt ggtggcgtgc ctgacgggta
    45421 tgacatcgct tctggcgcat cgctcggcgg ctgtttttca tgacggcatc cgcccgatcc
    45481 tgccgcaact gattgaaggc tatatgaatc gtcgcgaggc ggggagtatc gcttttggtc
    45541 tgagcattgg ttttgtggcc tcggtgggga tctcttttac cctgaaaacc gggctgctca
    45601 acgcatggtt actctttctt cctaccgata tcctcggcgt actggcgata aacagcctga
    45661 tggcgtttgg tcttggcgct atctggggcg tgttgatcct tacttgcctg ttgccagtaa
    45721 accagctgct gaccgcgctg ccggtggatg tattaggtag cctcggggaa ttaagctcgc
    45781 cggtggtttc tgcttttgca ctgttcccgc tggtggcgat tttctaccag tttggctgga
    45841 agcaaagtct ggtcgccgcc gtggttgttc tgatgacccg tgtggtagtc gtgcgctatt
    45901 tcccacatct taaccctgaa tccatcgaaa tctttattgg catggtgatg ctgctgggga
    45961 tcgcgataac tcacgacctg cgtcatcgtg atgaaaatga catcgatgcc agcgggcttt
    46021 cggtgtttga agagcgcacg tcgcggatta tcaaaaactt accttatatc gccatcgtgg
    46081 gagcattgat tgccgccgtt gccagtatga agatttttgc tggtagcgaa gtttcgatct
    46141 tcacactgga gaaagcgtat tccgcaggcg tgacgccgga acaatcgcaa acgctgatta
    46201 atcaggcggc tctggcagaa tttatgcgcg gactgggttt tgtgccgttg attgccacca
    46261 ccgcgttagc cactggcgtg tatgcagttg cgggctttac ctttgtttat gcggtgggct
    46321 atctctcgcc aaatccgatg gttgcggcgg tattaggcgc agtggttatt tcggcggaag
    46381 tcttgctgct tcgttcgatc ggcaaatggc tgggacgcta cccgtcggtg cgtaatgcgt
    46441 cggataacat ccgtaacgcc atgaatatgc tgatggaagt ggcgctgctg gtcggttcga
    46501 ttttcgcagc aattaagatg gcgggttata ccggattctc tatcgcggtt gccatttact
    46561 tcctcaacga atccctgggc cgtccggtac agaaaatggc ggcaccggtc gtggcagtaa
    46621 tgatcaccgg tattctgctg aatgttcttt actggcttgg cctgttcgtt ccggcttaag
    46681 gaaggcacct atgaagacgt ttcctctgca aagcctgacg attattgagg cgcagcaaaa
    46741 gcagtttgcg ctggtggata gcatttgtcg ccatttcccc ggcagcgagt ttcttactgg
    46801 cggtgattta gggttaactc caggtctgaa tcaaccgcgt attacccaac gggtggagca
    46861 ggtgctggct gatgcatttc acgcacaggc tgcggcgctg gtgcagggcg cggggactgg
    46921 cgcgattcgc gccgggctgg cggctttgct caaaccgggg cagcgtcttc tggtgcatga
    46981 cgcgcctgtt tacccgacga cacgggttat tattgagcag atggggctga cgcttattac
    47041 tgttgatttc aatgacctgt cggcactgaa gcaggtcgtc gacgagcaac aaccggatgc
    47101 ggcgctggtg cagcatacgc gccagcagcc gcaggacagc tacgtgctgg cagatgtgct
    47161 ggcaacgttg cgcgcggcag gtgttccagc gttaaccgat gacaactatg cggtgatgaa
    47221 ggtggcgcga atcggctgtg aatgcggcgc gaatgtctcg acattttcct gcttcaagct
    47281 atttgggcca gagggtgttg gtgcagtggt cggcgatgct gatgttatca accgtattcg
    47341 cgccacgctt tactccggcg gtagccagat ccagggcgca caggcgctgg aagtattgcg
    47401 tggtctggtg tttgcgccag tgatgcacgc ggtgcaggca ggggtatctg aacggttgct
    47461 ggctttgctt aacggtggtg cggtgccgga agtgaaaagc gcggtgattg ctaatgcgca
    47521 gtcgaaggtg ttgattgtcg agtttcatca gccgattgcc gccagagtgc tggaagaggc
    47581 gcaaaagcgc ggtgccttgc cttacccggt gggtgcagag tcgaaatatg aaatcccgcc
    47641 gctcttttat cgcctttccg gaacgtttcg ccaggcgaat ccacaatcag aacattgtgc
    47701 gattcgcatt aacccgaatc gcagcggtga agagacggtg ctgcggattt tgcgtgagag
    47761 tattgccagt atttaatgct ggatagcacc gtcaagctaa attccgtact gaacggtccc
    47821 ctcgcccctt tggggagagg gttagggtga ggggaacgta cgggatgtaa aattcaacgc
    47881 gccagcatcg ccggatgcga tgctggcgca tcttatccgg cctacggtta atgtctttta
    47941 ttatcaatcg ttaaggtgat gcgattggcg cggcagaaga gttcggagac gtgcaccggt
    48001 ttgtcattct gatcaaaggc gattttgctg atgcgaaaca acggttcgcc cagttcgcat
    48061 ttcagccatt ttgcctgcgg gcgggtggcg gcaaagatat cgatggtctt tttgtcgctg
    48121 accactcgcg tgtcgaaacg ctcctgaaat aactgataag tggagcttcc ttcaacgtaa
    48181 atctcatcaa agtcaggata acgcgacagt gggatccagg aactatcaat aaacagcggc
    48241 tctttatcga gatacatcac ccggcaaaga tggaacactt cgctgttgcc agggatgttc
    48301 agcttttcgc aaaacggcgc ggcgctgact cgttcctgct cgatcacttt ctctttcgtc
    48361 gccttgcctt gtgagacgcc aaaatcggta aaaccactga cggtcagcag ggcgttttcg
    48421 accttctggc tttgtacaaa ggtgcctttg ccctgccagc ggatcagtac gccgtctgcc
    48481 actaagtcgc tgatagcttt gcgaatagta atgcggctga cgttatattg cgtacaaagc
    48541 tcgttttcgg tagggatctg ttgcccggcc tggtaaaccc cctgcgcgat atcatccagc
    48601 aaccgctggc ggacggtagc gtagaggagt tgatgagagt agcggtccgt agctgacata
    48661 atcgaccctg aaataaagaa tgaattactc ggattatagg gtcgttttcc ggcaagtaac
    48721 agcacgttgg cgtgaagatg ctcacgccaa cgttatacct accaggcacc gtggtactga
    48781 atggttttcg ccgcgcacgc cgttccctgc gccatcgcct gcggtaatgt catccccgca
    48841 gaccagccgc aaaggaatcc ggcaatgaac gaatctccgg cacccatggt gtcgataacc
    48901 gtcaccggtt caggagcctg acgccagaac tgcgcgccat cccaggcaat gctgccgttt
    48961 tcacccagcg tgacaatcac cacgcctgcg ccacgggcaa caatcgcttt catcttcaga
    49021 cgcagcgctt cgtcttcttg cggtgcggag gcaaaggcaa aatcgagatg cggcaccagt
    49081 gtctgccaga gcgagctgtc ccacttgtcg gagaagtcga aagcggtaag tttgcccgca
    49141 gcgtgcagct gtgggaatgc gtcttccgca tgtccccaga ttgccgcgtg cacaatgtca
    49201 tactgcgcca gccaggcgta atcctcttca ctcagggcaa agtcggccat cacgccttcg
    49261 gtgtagtcgc caaaaacgcg atcattgtcg tgcagttcca cctgagtttg tgcggtaacg
    49321 ccgtgtttcg tatggacatg gctgatatcg acgcccatgc gggcgagatc ctgcttcagc
    49381 tttgtgccgt agtcatcgtc acccacccag gtaatgcatc ccggctgtat gccgtagcga
    49441 gtgcagtaca ccgccacatt gaccgcatta ccgccagaaa acgctttatt cagttgcggg
    49501 tagatatcga cgcagttatc gccgattgtc gccagggttt tcatctctca tcctccggca
    49561 gcagcgcgcg aaagcgttcc agcgcctggc gggcatagag tctgggctcg ttcatataca
    49621 tcgtcaccag ctccaccgta cagtaaccct catagccccg gtcgataata tcgcgcatca
    49681 gttccctcag cggcattttg ccttctccag gaatgtaatg cgtgtcgctg gccccgtcgc
    49741 tgtcgacaat atgcagatga cgtaatttat cgcccagttt gtcgaaataa ctcatcaccg
    49801 gttccgcctg gacatacggc gcgcaaatgt cgaccatgct gaacaggcgc ggcgaaggca
    49861 ccagcgccag cgcatgaagc acatcattag cattacacac gacgttcgat tcatacggcg
    49921 ttaacggttc gaggattaga tccatgccga tgttttccgc gtactcacac agttcgctta
    49981 ggttctctgc cagccgtccc cagataacat taggtggcgt gagatagccc gcgtgggccg
    50041 cggaaatcag cgtataaccc gcgttcatct cttttgccat atccatcgcc agcttgatca
    50101 tgtcgaggct ttcgcgacgc atatgttcat cgcccagcat catgttatac ggatagccgt
    50161 tggtttctgg cgtatagccg ataatcggca tctgatacgt ctgcgccagc gccttgattt
    50221 gtttgatgcc gcccgctttt aagtccggcg cgaacgcgtg cgggcgaccg ccccaaattt
    50281 cgatgccgtc gtaacccagc tcgcttgcat cacgaaatgc gtgttcaata ggcagccgct
    50341 ggtggccgca ggtaaacata cctgttttca tcgggtaact ccttgcgtaa attcggcccg
    50401 ttgccgggcc gggagaggga ttaatattcc accagaccac cgtaatagcg gcgttcatcc
    50461 gggttgtgat ctttgtaaat agacaggtag tagcagagcc actccattgg cacgaacatc
    50521 aggaacggtg ccagccacgg gtgcagccct tgcgaaattt cggcgtaatc gatgacgatc
    50581 acgttgtcag tacgctgttt aacaaagtta atggcgcgtt cggtggtgtg gcgactttca
    50641 tcattgccga gcaggaacag gaacggaacg cccggttcga caatctccag cgggccatgg
    50701 cggaactctc cgctctcaat cacgcagccg tgcgtccagg taaattccat cagcgttaca
    50761 atgccttctt tgtaacccag cggacgcagc ggacccgcag caacggtata aatcatcggc
    50821 cactggctgg ccagttcacc aagctggcgg cctttttctt cccaggtgcg taccagttga
    50881 ccgagcgcat tcggcaactg cttgagatcg tttttgatct tgccgatttc cgcgttcggc
    50941 gcgaggcggg tgatcatctc cagcaccacg ctgtagcaga gcagcaggtg aatttcccag
    51001 atacagtcgg cctgataatc aatgctaaat tccgccgccg aggtaatcgg gctgtccgcg
    51061 cgtttggtga acgccgcagt gagtgcgccg caggcccggc ccagctccag cgctttgatt
    51121 acctcttccg ttttaccgta gtcagaaacg ccaatcaccg cgcaacgatc gtcgaggcga
    51181 tacggggtgt tatcgcagaa ctcccagccg gaaatggcgt agacctgaag atcggaaaag
    51241 cgatccgcca gatgtttcgc cgtttgcgcc gcgttgagtg gcgatccgca ggcaacgaaa
    51301 taaatacgat caatgtcgcg cttcaccatc tcttccacga tggtgtgcac cagcggaacg
    51361 tcatggctga gaactttttc cacttcctga accatatttt cggtgaccag aaagtccacg
    51421 gtgcttttat caatatccaa cattttattt tctccagata acattacgac aaagcgttga
    51481 gctggcggct acgcttcgcc cagaaggcgt aagcaggcag accagtagca ataacgatga
    51541 cggcgcagat aaggccggga atcggtgccc agacaaaggt tgaggcgacc agaatgaggc
    51601 ttgacgcaat ggcgagggtg gtcatcagcc cgaaagccgg agtacgccac agcggtttgt
    51661 aatcgtcgcg tttacgacac cagatgatgg agccgaaggt gagggtattt ttgaaacaca
    51721 tcaccagggt gaaataaccc agcaggctgg tgagatcgga aacgaagatg aagaagatcc
    51781 ccagcgcccc ttgcaggatg atggagacat ccggcgtgtt gtatttcgga tgcacatggc
    51841 cgaagcattt aaagaacagg ttgtctttcg ccatcgcgta ttccagccgc ggctggtaca
    51901 tcacgcagct ggaaagcgaa ccaagaatga cgatcatcgc cgtgatggca acaaagatcc
    51961 cagcggtgct gccgagtgcg gggatccagg tcagggcgtc ggaaatcggc gtttcagaat
    52021 tggcgagttt gtcgaagggc atcaggccgg aaatcaccag cgccagcagg gtgtagagca
    52081 ccagaaccag cagacaggaa ccaatcagcg ctcgtggcat ggtttttccg gggtttttaa
    52141 tttcgccggt catataacag atagaggcca tgccggtata cgaccaactg gtggcagaga
    52201 tccccgccag cagcgccata aagctgcccg ttgcgccaat cgcagtggtg gtaggggcgg
    52261 caaaattctc cgctttaaac cagaagatcc caaggccaat gacgatagtg aacgggataa
    52321 ttttggcgat ggtaattagc gtctgaaacg ctgcgccgcc ttcaacggag cgcaggtgta
    52381 gcaacataaa ggcgataatt aatccggcgg cgataaattt accgagcaac ggatcgatag
    52441 gcgttaaaaa gccaagattg ctgacaatcg ccagcgccat aatcgacaat gacggcgcat
    52501 cgttggccca gaagctggcc cagccggaga ggaaagccag cggtcggctt ccggcatttt
    52561 tcagataaac ataatctgcg ccattttccg gataagcggt ggatagttcc gcatagacgc
    52621 acatttgcgg gatcacaatt aacccgccaa tgacaaacgc gagcaccgta agccacggcg
    52681 tgcccgctgc ttttgccact tcacccacag atacaaaaat accggagccg acggttgtcc
    52741 cgacggcgat tgcaagaacg gcccaaaatc cgagcttgcg ttggagttcc tggcttccca
    52801 tagcgatacc tttttcttgt ccaatacgtt gtaggggaga gtttgcagtc atttaaaagg
    52861 taacatgaca atgcatgatg aatataacaa catatgatgt tatgtaatgt ggaggaagat
    52921 cacaaaattt cgcacaggat cgcgctgtgg ctaatggatg tagttatcaa attgaattta
    52981 aagtgaaaat atttttacgg gcgggggcaa gaaggacata gaaacaaata cgccctcgga
    53041 aaatccagag ggcgtcgggc aattaaaccg gtgttagccg atttctgtca gagacttact
    53101 gtgcagtagg accgcaaccg ccaatgattt tagaaataga aattgccggg tgcagcaggt
    53161 agtcgtaaga gcagttctta tcacggtttt caatatgacc agtacatgca gttaacgtag
    53221 ccagta
//
LOCUS       DAZKHF020000004        49752 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2201S31241
            SAMN43267800-rid25106413.denovo.004, whole genome shotgun sequence.
ACCESSION   DAZKHF020000004 DAZKHF020000000
VERSION     DAZKHF020000004.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267800
            Sequence Read Archive: SRR30307263
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 49752)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 49752)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 49752)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350581.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350581.2
            Genome Coverage       :: 151x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:21:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,652
            CDSs (total)                      :: 5,557
            Genes (coding)                    :: 5,320
            CDSs (with protein)               :: 5,320
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 237
            CDSs (without protein)            :: 237
            Pseudo Genes (ambiguous residues) :: 0 of 237
            Pseudo Genes (frameshifted)       :: 102 of 237
            Pseudo Genes (incomplete)         :: 130 of 237
            Pseudo Genes (internal stop)      :: 52 of 237
            Pseudo Genes (multiple problems)  :: 41 of 237
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..49752
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267800-rid25106413.denovo.004"
                     /serotype="O 26:H11"
                     /isolate="2201S31241"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-01"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            complement(65..1309)
                     /gene="mtr"
                     /locus_tag="ACHOSC_RS00780"
                     /old_locus_tag="pgaptmp_000156"
     CDS             complement(65..1309)
                     /gene="mtr"
                     /locus_tag="ACHOSC_RS00780"
                     /old_locus_tag="pgaptmp_000156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015173 - aromatic amino acid
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0015801 - aromatic amino acid transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan permease"
                     /protein_id="HGU4546172.1"
                     /translation="MATLTTTQTSPSLLGGVVIIGGTIIGAGMFSLPVVMSGAWFFWS
                     MAALIFTWFCMLHSGLMILEANLNYRIGSSFDTITKDLLGKGWNVVNGISIAFVLYIL
                     TYAYISASGSILHHTFAEMSLNVPARAAGFGFALLVAFVVWLSTKAVSRMTAIVLGAK
                     VITFFLTFGSLLGHVQPATLFNVAESNASYAPYLLMTLPFCLASFGYHGNVPSLMKYY
                     GKDPKTIVKCLVYGTLMALALYTIWLLATMGNIPRPEFIGIAEKGGNIDVLVQALSGV
                     LNSRSLDLLLVVFSNFAVASSFLGVTLGLFDYLADLFGFDDSALGRLKTALLTFAPPV
                     VGGLLFPNGFLYAIGYAGLAATIWAAIVPALLARASRKRFGSPKFRVWGGKPMITLIL
                     VFGVGNALVHILSSFNLLPVYQ"
     gene            complement(1463..3352)
                     /gene="deaD"
                     /locus_tag="ACHOSC_RS00785"
                     /old_locus_tag="pgaptmp_000157"
     CDS             complement(1463..3352)
                     /gene="deaD"
                     /locus_tag="ACHOSC_RS00785"
                     /old_locus_tag="pgaptmp_000157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417631.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RNA helicase DeaD"
                     /protein_id="HGU4546173.1"
                     /translation="MAEFETTFADLGLKAPILEALNDLGYEKPSPIQAECIPHLLNGR
                     DVLGMAQTGSGKTAAFSLPLLQNLDPELKAPQILVLAPTRELAVQVAEAMTDFSKHMR
                     GVNVVALYGGQRYDVQLRALRQGPQIVVGTPGRLLDHLKRGTLDLSKLSGLVLDEADE
                     MLRMGFIEDVETIMAQIPEGHQTALFSATMPEAIRRITRRFMKEPQEVRIQSSVTTRP
                     DISQSYWTVWGMRKNEALVRFLEAEDFDAAIIFVRTKNATLEVAEALERNGYNSAALN
                     GDMNQALREQTLERLKDGRLDILIATDVAARGLDVERISLVVNYDIPMDSESYVHRIG
                     RTGRAGRAGRALLFVENRERRLLRNIERTMKLTIPEVELPNAELLGKRRLEKFAAKVQ
                     QQLESSDLDQYRALLSKIQPTAEGEEQDLETLAAALLKMAQGERTLIVPPDAPMRPKR
                     EFRDRDDRGPRDRNDRGPRGDREDRPRRERRDVGDMQLYRIEVGRDDGVEVRHIVGAI
                     ANEGDISSRYIGNIKLFASHSTIELPKGMPGEVLQHFTRTRILNKPMNMQLLGDAQPH
                     TGGERRGGGRGFGGERREGGRNFSGERREGGRGDGRRFSGERREGRAPRRDDSTGRRR
                     FGGDA"
     gene            complement(3345..3425)
                     /gene="yrbN"
                     /locus_tag="ACHOSC_RS00790"
                     /old_locus_tag="pgaptmp_000158"
     CDS             complement(3345..3425)
                     /gene="yrbN"
                     /locus_tag="ACHOSC_RS00790"
                     /old_locus_tag="pgaptmp_000158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YrbN"
                     /protein_id="HGU4546174.1"
                     /translation="MKIADQFHDELCRLAAINFEAHVLHG"
     gene            complement(3532..4416)
                     /gene="nlpI"
                     /locus_tag="ACHOSC_RS00795"
                     /old_locus_tag="pgaptmp_000159"
     CDS             complement(3532..4416)
                     /gene="nlpI"
                     /locus_tag="ACHOSC_RS00795"
                     /old_locus_tag="pgaptmp_000159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020802045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein NlpI"
                     /protein_id="HGU4546175.1"
                     /translation="MKPFLRWCFVATALTLAGCSNTSWRKSEVLAVPLQPTLQQEVIL
                     ARMEQILASRALTDDERAQLLYERGVLYDSLGLRALARNDFSQALAIRPDMPEVFNYL
                     GIYLTQAGNFDAAYEAFDSVLELDPTYNYAHLNRGIALYYGGRDKLAQDDLLAFYQDD
                     PNDPFRSLWLYLAEQKLDEKQAKEVLKQHFEKSDKEQWGWNIVEFYLGNISEQTLMER
                     LKADATDNTSLAEHLSETNFYLGKYYLSLGDLDSATALFKLAVANNVHNFVEHRYALL
                     ELSLLGQDQDDLAESDQQ"
     gene            complement(4525..6660)
                     /gene="pnp"
                     /locus_tag="ACHOSC_RS00800"
                     /old_locus_tag="pgaptmp_000160"
     CDS             complement(4525..6660)
                     /gene="pnp"
                     /locus_tag="ACHOSC_RS00800"
                     /old_locus_tag="pgaptmp_000160"
                     /EC_number="2.7.7.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312072.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004654 - polyribonucleotide
                     nucleotidyltransferase activity [Evidence IEA];
                     GO_process: GO:0006402 - mRNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyribonucleotide nucleotidyltransferase"
                     /protein_id="HGU4546176.1"
                     /translation="MLNPIVRKFQYGQHTVTLETGMMARQATAAVMVSMDDTAVFVTV
                     VGQKKAKPGQDFFPLTVNYQERTYAAGRIPGSFFRREGRPSEGETLIARLIDRPIRPL
                     FPEGFVNEVQVIATVVSVNPQVNPDIVAMIGASAALSLSGIPFNGPIGAARVGYINDQ
                     YVLNPTQDELKESKLDLVVAGTEAAVLMVESEAELLSEDQMLGAVVFGHEQQQVVIQN
                     INELVKEAGKPRWDWQPEPVNEALNARVAALAEARLSDAYRITDKQERYAQVDVIKSE
                     TIATLLAEDETLDENELGEILHAIEKNVVRSRVLAGEPRIDGREKDMIRGLDVRTGVL
                     PRTHGSALFTRGETQALVTATLGTARDAQVLDELMGERTDTFLFHYNFPPYSVGETGM
                     VGSPKRREIGHGRLAKRGVLAVMPDMDKFPYTVRVVSEITESNGSSSMASVCGASLAL
                     MDAGVPIKAAVAGIAMGLVKEGDNYVVLSDILGDEDHLGDMDFKVAGSREGISALQMD
                     IKIEGITKEIMQVALNQAKGARLHILGVMEQAINAPRGDISEFAPRIHTIKINPDKIK
                     DVIGKGGSVIRALTEETGTTIEIEDDGTVKIAATDGEKAKHAIRRIEEITAEIEVGRV
                     YTGKVTRIVDFGAFVAIGGGKEGLVHISQIADKRVEKVTDYLQMGQEVPVKVLEVDRQ
                     GRIRLSIKEATEQSQPAAAPEAPAAEQGE"
     gene            complement(6907..7176)
                     /gene="rpsO"
                     /locus_tag="ACHOSC_RS00805"
                     /old_locus_tag="pgaptmp_000161"
     CDS             complement(6907..7176)
                     /gene="rpsO"
                     /locus_tag="ACHOSC_RS00805"
                     /old_locus_tag="pgaptmp_000161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004933502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S15"
                     /protein_id="HGU4546177.1"
                     /translation="MSLSTEATAKIVSEFGRDANDTGSTEVQVALLTAQINHLQGHFA
                     EHKKDHHSRRGLLRMVSQRRKLLDYLKRKDVARYTQLIERLGLRR"
     gene            complement(7325..8269)
                     /gene="truB"
                     /locus_tag="ACHOSC_RS00810"
                     /old_locus_tag="pgaptmp_000162"
     CDS             complement(7325..8269)
                     /gene="truB"
                     /locus_tag="ACHOSC_RS00810"
                     /old_locus_tag="pgaptmp_000162"
                     /EC_number="5.4.99.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(55) synthase TruB"
                     /protein_id="HGU4546178.1"
                     /translation="MSRPRRRGRDINGVLLLDKSQGMSSNDALQKVKRIYNANRAGHT
                     GALDPLATGMLPICLGEATKFSQYLLDSDKRYRVIARLGQRTDTSDADGQIVEERPVT
                     FSAEQLAAALDTFRGDIEQIPSMYSALKYQGKKLYEYARQGIEVPREARPITVYELLF
                     IRHEGNELELEIHCSKGTYIRTIIDDLGEKLGCGAHVIYLRRLAVSKYPVERMVTLEH
                     LRELVEQAEQQDIPAAELLDPLLMPMDSPASDYPVVNLPLTSSVYFKNGNPVRTSGAP
                     LEGLVRVTEGENGKFIGMGEIDDEGRVAPRRLVVEYPA"
     gene            complement(8269..8670)
                     /gene="rbfA"
                     /locus_tag="ACHOSC_RS00815"
                     /old_locus_tag="pgaptmp_000163"
     CDS             complement(8269..8670)
                     /gene="rbfA"
                     /locus_tag="ACHOSC_RS00815"
                     /old_locus_tag="pgaptmp_000163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001712908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosome-binding factor RbfA"
                     /protein_id="HGU4546179.1"
                     /translation="MAKEFGRPQRVAQEMQKEIALILQREIKDPRLGMMTTVSGVEMS
                     RDLAYAKVYVTFLNDKDEDAVKAGIKALQEASGFIRSLLGKAMRLRIVPELTFFYDNS
                     LVEGMRMSNLVTSVVKHDEERRVNPDDSKED"
     gene            complement(8834..11506)
                     /gene="infB"
                     /locus_tag="ACHOSC_RS00820"
                     /old_locus_tag="pgaptmp_000164"
     CDS             complement(8834..11506)
                     /gene="infB"
                     /locus_tag="ACHOSC_RS00820"
                     /old_locus_tag="pgaptmp_000164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-2"
                     /protein_id="HGU4546180.1"
                     /translation="MTDVTIKTLAAERQTSVERLVQQFADAGIRKSADDSVSAQEKQT
                     LIDHLNQKNSGPDKLTLQRKTRSTLNIPGTGGKSKSVQIEVRKKRTFVKRDPQEAERL
                     AAEEQAQREAEEQARREAEESAKREAQQKAEREAAEQAKREAAEQAKREAAEKDKVSN
                     QQDDMTKNAQAEKARREQEAAELKRKAEEEARRKLEEEARRVAEEARRMAEENKWTDN
                     AEPTEDSSDYHVTTSQHARQAEDESDREVEGGRGRGRNAKAARPKKGNKHAESKADRE
                     EARAAVRGGKGGKRKGSSLQQGFQKPAQAVNRDVVIGETITVGELANKMAVKGSQVIK
                     AMMKLGAMATINQVIDQETAQLVAEEMGHKVILRRENELEEAVMSDRDTGAAAEPRAP
                     VVTIMGHVDHGKTSLLDYIRSTKVASGEAGGITQHIGAYHVETENGMITFLDTPGHAA
                     FTSMRARGAQATDIVVLVVAADDGVMPQTIEAIQHAKAAQVPVVVAVNKIDKPEADPD
                     RVKNELSQYGILPEEWGGESQFVHVSAKAGTGIDELLDAILLQAEVLELKAVRKGMAS
                     GAVIESFLDKGRGPVATVLVREGTLHKGDIVLCGFEYGRVRAMRNELGQEVLEAGPSI
                     PVEILGLSGVPAAGDEVTVVRDEKKAREVALYRQGKFREVKLARQQKSKLENMFANMT
                     EGEVHEVNIVLKADVQGSVEAISDSLLKLSTDEVKVKIIGSGVGGITETDATLAAASN
                     AILVGFNVRADASARKVIEAESLDLRYYSVIYNLIDEVKAAMSGMLSPELKQQIIGLA
                     EVRDVFKSPKFGAIAGCMVTEGVVKRHNPIRVLRDNVVIYEGELESLRRFKDDVNEVR
                     NGMECGIGVKNYNDVRTGDVIEVFEIIEIQRTIA"
     gene            complement(11531..13018)
                     /gene="nusA"
                     /locus_tag="ACHOSC_RS00825"
                     /old_locus_tag="pgaptmp_000165"
     CDS             complement(11531..13018)
                     /gene="nusA"
                     /locus_tag="ACHOSC_RS00825"
                     /old_locus_tag="pgaptmp_000165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005131833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor NusA"
                     /protein_id="HGU4546181.1"
                     /translation="MNKEILAVVEAVSNEKALPREKIFEALESALATATKKKYEQEID
                     VRVQIDRKSGDFDTFRRWLVVDEVTQPTKEITLEAARYEDESLNLGDYVEDQIESVTF
                     DRITTQTAKQVIVQKVREAERAMVVDQFREHEGEIITGVVKKVNRDNISLDLGNNAEA
                     VILREDMLPRENFRPGDRVRGVLYSVRPEARGAQLFVTRSKPEMLIELFRIEVPEIGE
                     EVIEIKAAARDPGSRAKIAVKTNDKRIDPVGACVGMRGARVQAVSTELGGERIDIVLW
                     DDNPAQFVINAMAPADVASIVVDEDKHTMDIAVEAGNLAQAIGRNGQNVRLASQLSGW
                     ELNVMTVDDLQAKHQAEAHAAIDTFTKYLDIDEDFATVLVEEGFSTLEELAYVPMKEL
                     LEIEGLDEPTVEALRERAKNALATIAQAQEESLGDNKPADDLLNLEGIDRDLAFKLAA
                     RGVCTLEDLAEQGIDDLADIEGLTDEKAGALIMAARNICWFGDEA"
     gene            complement(13046..13498)
                     /gene="rimP"
                     /locus_tag="ACHOSC_RS00830"
                     /old_locus_tag="pgaptmp_000166"
     CDS             complement(13046..13498)
                     /gene="rimP"
                     /locus_tag="ACHOSC_RS00830"
                     /old_locus_tag="pgaptmp_000166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004125659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0042274 - ribosomal small subunit
                     biogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimP"
                     /protein_id="HGU4546182.1"
                     /translation="MSTLEQKLTEMITAPVEALGFELVGIEFIRGRTSTLRIYIDSED
                     GINVDDCADVSHQVSAVLDVEDPITVAYNLEVSSPGLDRPLFTAEHYARFVGEEVTLV
                     LRMAVQNRRKWQGVIKAVDGEMITVTVEGKDEVFALSNIQKANLVPHF"
     gene            complement(13705..13781)
                     /locus_tag="ACHOSC_RS00835"
                     /old_locus_tag="pgaptmp_000167"
     tRNA            complement(13705..13781)
                     /locus_tag="ACHOSC_RS00835"
                     /old_locus_tag="pgaptmp_000167"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(13745..13747),aa:Met,seq:cat)
     gene            14129..15472
                     /gene="argG"
                     /locus_tag="ACHOSC_RS00840"
                     /old_locus_tag="pgaptmp_000168"
     CDS             14129..15472
                     /gene="argG"
                     /locus_tag="ACHOSC_RS00840"
                     /old_locus_tag="pgaptmp_000168"
                     /EC_number="6.3.4.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004055 - argininosuccinate synthase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA];
                     GO_process: GO:0006526 - arginine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate synthase"
                     /protein_id="HGU4546183.1"
                     /translation="MTTILKHLPVGQRIGIAFSGGLDTSAALLWMRQKGAVPYAYTAN
                     LGQPDEEDYDAIPRRAMEYGAENARLIDCRKQLVAEGIAAIQCGAFHNTTGGLTYFNT
                     TPLGRAVTGTMLVAAMKEDGVNIWGDGSTYKGNDIERFYRYGLLTNAELQIYKPWLDT
                     DFIDELGGRHEMSEFMIACGFDYKMSVEKAYSTDSNMLGATHEAKDLEYLNSSVKIVN
                     PIMGVKFWDESVKIPAEEVTVRFEQGHPVALNGKTFSDDVEMMLEANRIGGRHGLGMS
                     DQIENRIIEAKSRGIYEAPGMALLHIAYERLLTGIHNEDTIEQYHAHGRQLGRLLYQG
                     RWFDSQALMLRDSLQRWVASQITGEVTLELRRGNDYSILNTVSENLTYKPERLTMEKG
                     DSVFSPDDRIGQLTMRNLDITDTREKLFGYAKTGLLSSSATSGVPQVENMENKGQ"
     gene            complement(15480..17105)
                     /gene="yhbX"
                     /locus_tag="ACHOSC_RS00845"
                     /old_locus_tag="pgaptmp_000169"
     CDS             complement(15480..17105)
                     /gene="yhbX"
                     /locus_tag="ACHOSC_RS00845"
                     /old_locus_tag="pgaptmp_000169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417641.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase-like hydrolase/transferase"
                     /protein_id="HGU4546184.1"
                     /translation="MTVFNKFARSFKSHWLLYLCVIVFGITNLVASSGAHMVQRLLFF
                     VLTILVVKRISSLPLRLLVAAPFVLLTAADMSISLYSWCTFGTTFNDGFAISVLQSDP
                     DEVVKMLGMYIPYLCAFAFLSLLFLAVIIKYDVSLPTKKVTGILLLIVISGSLFSACQ
                     FAYKDAKNKKAFSPYILASRFATYTPFFNLNYFALAAKEHQRLLSIANTVPYFQLSVR
                     DTGIDTYVLIVGESVRVDNMSLYGYTRSTTPQVEAQRKQIKLFNQAISGAPYTALSVP
                     LSLTADSVLSHDIHNYPDNIINMANQAGFQTFWLSSQSAFRQNGTAVTSIAMRAMETV
                     YVRGFDELLLPHLSQALQQNTQQKKLIVLHLNGSHEPACSAYPQSSAVFQPQDDQDAC
                     YDNSIHYTDSLLGQVFELLKDRRASVMYFADHGLERDPTKKNVYFHGGREASQQAYHV
                     PMFIWYSPVLGDGVDRTTENNIFSTAYNNYLINAWMGVTKPEQPQTLEEVIAHYKGDS
                     RVVDANHDVFDYVMLRKEFTEDKQGNPTPEGQG"
     gene            complement(17564..17650)
                     /locus_tag="ACHOSC_RS00850"
                     /old_locus_tag="pgaptmp_000170"
     tRNA            complement(17564..17650)
                     /locus_tag="ACHOSC_RS00850"
                     /old_locus_tag="pgaptmp_000170"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(17614..17616),aa:Leu,seq:gag)
     gene            complement(17665..17997)
                     /gene="secG"
                     /locus_tag="ACHOSC_RS00855"
                     /old_locus_tag="pgaptmp_000171"
     CDS             complement(17665..17997)
                     /gene="secG"
                     /locus_tag="ACHOSC_RS00855"
                     /old_locus_tag="pgaptmp_000171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015136 - sialic acid transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015739 - sialic acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecG"
                     /protein_id="HGU4546185.1"
                     /translation="MYEALLVVFLIVAIGLVGLIMLQQGKGADMGASFGAGASATLFG
                     SSGSGNFMTRMTALLATLFFIISLVLGNINSNKTNKGSEWENLSAPVKTEQTQPAAPA
                     KPTSDIPN"
     gene            complement(18225..19562)
                     /gene="glmM"
                     /locus_tag="ACHOSC_RS00860"
                     /old_locus_tag="pgaptmp_000172"
     CDS             complement(18225..19562)
                     /gene="glmM"
                     /locus_tag="ACHOSC_RS00860"
                     /old_locus_tag="pgaptmp_000172"
                     /EC_number="5.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008966 - phosphoglucosamine mutase
                     activity [Evidence IEA];
                     GO_function: GO:0016868 - intramolecular
                     phosphotransferase activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglucosamine mutase"
                     /protein_id="HGU4546186.1"
                     /translation="MSNRKYFGTDGIRGRVGDAPITPDFVLKLGWAAGKVLARHGSRK
                     IIIGKDTRISGYMLESALEAGLAAAGLSALFTGPMPTPAVAYLTRTFRAEAGIVISAS
                     HNPFYDNGIKFFSIDGTKLPDAVEEAIEAEMEKEISCVDSAELGKASRIVDAAGRYIE
                     FCKATFPNELSLSELKIVVDCANGATYHIAPNVLRELGANVIAIGCEPNGVNINAEVG
                     ATDVRALQARVLAEKADLGIAFDGDGDRVIMVDHEGNKVDGDQIMYIIAREGLRQGQL
                     RGGAVGTLMSNMGLELALKQLGIPFARAKVGDRYVLEKMQEKGWRIGAENSGHVILLD
                     KTTTGDGIVAGLQVLAAMARNHMSLHDLCSGMKMFPQILVNVRYTAGSGDPLEHESVK
                     AVTAEVEAALGNRGRVLLRKSGTEPLIRVMVEGEDEAQVTEFAHRIADAVKAV"
     gene            complement(19555..20403)
                     /gene="folP"
                     /locus_tag="ACHOSC_RS00865"
                     /old_locus_tag="pgaptmp_000173"
     CDS             complement(19555..20403)
                     /gene="folP"
                     /locus_tag="ACHOSC_RS00865"
                     /old_locus_tag="pgaptmp_000173"
                     /EC_number="2.5.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312083.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004156 - dihydropteroate synthase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydropteroate synthase"
                     /protein_id="HGU4546187.1"
                     /translation="MKLFAQGTSLDLSHPHVMGILNVTPDSFSDGGTHNSLIDAVKHA
                     NLMINAGATIIDVGGESTRPGAAEVSVEEELQRVIPVVEAIAQRFEVWISVDTSKPEV
                     IRESAKVGAHIINDIRSLSEPGALEAAAETGLPVCLMHMQGNPKTMQEAPKYDDVFAE
                     VNRYFIEQIARCEQAGIAKEKLLLDPGFGFGKNLSHNYSLLARLAEFHHFNLPLLVGM
                     SRKSMIGQLLNVGPSERLSGSLACAVIAAMQGAHIIRVHDVKETVEAMRVVEATLSAK
                     ENKRYE"
     gene            complement(20493..22427)
                     /gene="ftsH"
                     /locus_tag="ACHOSC_RS00870"
                     /old_locus_tag="pgaptmp_000174"
     CDS             complement(20493..22427)
                     /gene="ftsH"
                     /locus_tag="ACHOSC_RS00870"
                     /old_locus_tag="pgaptmp_000174"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014833437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004176 - ATP-dependent peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent zinc metalloprotease FtsH"
                     /protein_id="HGU4546188.1"
                     /translation="MAKNLILWLVIAVVLMSVFQSFGPSESNGRKVDYSTFLQEVNND
                     QVREARINGREINVTKKDSNRYTTYIPVQDPKLLDNLLTKNVKVVGEPPEEPSLLASI
                     FISWFPMLLLIGVWIFFMRQMQGGGGKGAMSFGKSKARMLTEDQIKTTFADVAGCDEA
                     KEEVAELVEYLREPSRFQKLGGKIPKGVLMVGPPGTGKTLLAKAIAGEAKVPFFTISG
                     SDFVEMFVGVGASRVRDMFEQAKKAAPCIIFIDEIDAVGRQRGAGLGGGHDEREQTLN
                     QMLVEMDGFEGNEGIIVIAATNRPDVLDPALLRPGRFDRQVVVGLPDVRGREQILKVH
                     MRRVPLAPDIDAAIIARGTPGFSGADLANLVNEAALFAARGNKRVVSMVEFEKAKDKI
                     MMGAERRSMVMTEAQKESTAYHEAGHAIIGRLVPEHDPVHKVTIIPRGRALGVTFFLP
                     EGDAISASRQKLESQISTLYGGRLAEEIIYGPEHVSTGASNDIKVATNLARNMVTQWG
                     FSEKLGPLLYAEEEGEVFLGRSVAKAKHMSDETARIIDQEVKALIERNYNRARQLLTD
                     NMDILHAMKDALMKYETIDAPQIDDLMARRDVRPPAGWEEPGASNNSGDNGSPKAPRP
                     VDEPRTPNPGNTMSEQLGDK"
     gene            complement(22527..23156)
                     /gene="rlmE"
                     /locus_tag="ACHOSC_RS00875"
                     /old_locus_tag="pgaptmp_000175"
     CDS             complement(22527..23156)
                     /gene="rlmE"
                     /locus_tag="ACHOSC_RS00875"
                     /old_locus_tag="pgaptmp_000175"
                     /EC_number="2.1.1.166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016436 - rRNA (uridine) methyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0001510 - RNA methylation [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uridine(2552)-2'-O)-methyltransferase
                     RlmE"
                     /protein_id="HGU4546189.1"
                     /translation="MTGKKRSASSSRWLQEHFSDKYVQQAQKKGLRSRAWFKLDEIQQ
                     SDKLFKPGMTVVDLGAAPGGWSQYVVTQIGGKGRIIACDLLPMDPIVGVDFLQGDFRD
                     ELVMKALLERVGDSKVQVVMSDMAPNMSGTPAVDIPRAMYLVELALEMCRDVLAPGGS
                     FVVKVFQGEGFDEYLREIRSLFTKVKVRKPDSSRARSREVYIVATGRKP"
     gene            23282..23575
                     /gene="yhbY"
                     /locus_tag="ACHOSC_RS00880"
                     /old_locus_tag="pgaptmp_000176"
     CDS             23282..23575
                     /gene="yhbY"
                     /locus_tag="ACHOSC_RS00880"
                     /old_locus_tag="pgaptmp_000176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014833438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome assembly RNA-binding protein YhbY"
                     /protein_id="HGU4546190.1"
                     /translation="MNLSTKQKQHLKGLAHPLKPVVLLGSNGLTEGVLAEIEQALEHH
                     ELIKVKIATEDRETKTLIVEAIVRETGACNVQVIGKTLVLYRPTKERKISLPR"
     gene            complement(23731..24207)
                     /gene="greA"
                     /locus_tag="ACHOSC_RS00885"
                     /old_locus_tag="pgaptmp_000177"
     CDS             complement(23731..24207)
                     /gene="greA"
                     /locus_tag="ACHOSC_RS00885"
                     /old_locus_tag="pgaptmp_000177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006178768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0070063 - RNA polymerase binding [Evidence
                     IEA];
                     GO_process: GO:0032784 - regulation of DNA-templated
                     transcription elongation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription elongation factor GreA"
                     /protein_id="HGU4546191.1"
                     /translation="MQAIPMTLRGAEKLREELDFLKSVRRPEIIAAIAEAREHGDLKE
                     NAEYHAAREQQGFCEGRIKDIEAKLSNAQVIDVTKMPNNGRVIFGATVTVLNLDSDEE
                     QTYRIVGDDEADFKQNLISVNSPIARGLIGKEEDDVVVIKTPGGEVEFEVIKVEYL"
     gene            24455..25888
                     /gene="dacB"
                     /locus_tag="ACHOSC_RS00890"
                     /old_locus_tag="pgaptmp_000178"
     CDS             24455..25888
                     /gene="dacB"
                     /locus_tag="ACHOSC_RS00890"
                     /old_locus_tag="pgaptmp_000178"
                     /EC_number="3.4.16.4"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001212660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004185 - serine-type carboxypeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine-type D-Ala-D-Ala carboxypeptidase"
                     /protein_id="HGU4546192.1"
                     /translation="MRFSRFIIGLTSCIAFSVQAANVDEYVTQLPAGANLALMVQKVG
                     ASAPAIDYHSQQMALPASTQKVITALAALIQLGPDFRFTTTLETKGNVENGVLKGDLV
                     ARFGADPTLKRQDIRNMVATLKKSGVNQIDGNVLIDTSIFASHDKAPGWPWNDMTQCF
                     SAPPAAAIVDRNCFSVSLYSAPKPGDMAFIRVASYYPVTMFSQVRTLPRGSAEAQYCE
                     LDVVPGDLNRFTLTGCLPQRSEPLPLAFAVQDGASYAGAILKDELKQAGITWSGTLLR
                     QTQVNEPGTVVASKQSAPLHDLLKIMLKKSDNMIADTVFRMIGHARFNVPGTWRAGSD
                     AVRQILRQQAGVDIGNTIIADGSGLSRHNLIAPATMMQVLQYIAQHDNELNFISMLPL
                     AGYDGSLQYRAGLHQAGVDGKVSAKTGSLQGVYNLAGFITTASGQRMAFVQYLSGYAV
                     EPADQRNRRIPLVRFESRLYKDIYQNN"
     gene            complement(25928..27100)
                     /gene="cgtA"
                     /locus_tag="ACHOSC_RS00895"
                     /old_locus_tag="pgaptmp_000179"
     CDS             complement(25928..27100)
                     /gene="cgtA"
                     /locus_tag="ACHOSC_RS00895"
                     /old_locus_tag="pgaptmp_000179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Obg family GTPase CgtA"
                     /protein_id="HGU4546193.1"
                     /translation="MKFVDEASILVVAGDGGNGCVSFRREKYIPKGGPDGGDGGDGGD
                     VWMEADENLNTLIDYRFEKSFRAERGQNGASRDCTGKRGKDVTIKVPVGTRIIDQGTG
                     ETMGDMTKHGQRLLVAKGGWHGLGNTRFKSSVNRTPRQKTNGTPGDKRELLLELMLLA
                     DVGMLGMPNAGKSTFIRAVSAAKPKVADYPFTTLVPSLGVVRMDNEKSFVVADIPGLI
                     EGAAEGAGLGIRFLKHLERCRVLLHLIDIDPIDGTDPVENARIIISELEKYSQDLAAK
                     PRWLVFNKIDLLDKAEAEEKAKAIAEALGWEDKYYLISAASGLGVKDLCWDVMTFIIE
                     NPVVQAEEAKQPEKVEFMWDDYHRQQLEEIAEEDDEDWDDDWDEDDEEGVEFIYKR"
     gene            complement(27116..28081)
                     /gene="yhbE"
                     /locus_tag="ACHOSC_RS00900"
                     /old_locus_tag="pgaptmp_000180"
     CDS             complement(27116..28081)
                     /gene="yhbE"
                     /locus_tag="ACHOSC_RS00900"
                     /old_locus_tag="pgaptmp_000180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012135318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HGU4546194.1"
                     /translation="MKQQAGIGILLALTTAICWGALPIAMKQVLEVMEPPTIVFYRFL
                     MASIGLGAILAVKKRLPPLRVFRKPRWLILLAVATAGLFGNFILFSSSLQYLSPTASQ
                     VIGQLSPVGMMVASVFILKEKMRSTQVVGALMLLSGLVMFFNTSLVEIFTKLTDYTWG
                     VIFGVGAATVWVSYGVAQKVLLRRLASPQILFLLYALCTIALFPLAKPGVIAQLSHWQ
                     LACLIFCGLNTLVGYGALAEAMARWQAAQVSAIITLTPLFTLFFSDLLSLAWPDFFAR
                     PMLNLLGYLGAFVVVAGAMYSAIGHRIWGGLRKHTTVVSQPRAGE"
     gene            complement(28208..28465)
                     /gene="rpmA"
                     /locus_tag="ACHOSC_RS00905"
                     /old_locus_tag="pgaptmp_000181"
     CDS             complement(28208..28465)
                     /gene="rpmA"
                     /locus_tag="ACHOSC_RS00905"
                     /old_locus_tag="pgaptmp_000181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004933559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L27"
                     /protein_id="HGU4546195.1"
                     /translation="MAHKKAGGSTRNGRDSEAKRLGVKRFGGESVLAGSIIVRQRGTK
                     FHAGANVGCGRDHTLFAKADGKVKFEVKGPKNRKFISIEAE"
     gene            complement(28486..28797)
                     /gene="rplU"
                     /locus_tag="ACHOSC_RS00910"
                     /old_locus_tag="pgaptmp_000182"
     CDS             complement(28486..28797)
                     /gene="rplU"
                     /locus_tag="ACHOSC_RS00910"
                     /old_locus_tag="pgaptmp_000182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015960603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L21"
                     /protein_id="HGU4546196.1"
                     /translation="MYAVFQSGGKQHRVSEGQTVRLEKLDIATGETVEFAEVLMIANG
                     EEVKIGVPFVDGGVIKAEVVAHGRGEKVKIVKFRRRKHYRKQQGHRQWFTDVKITGIS
                     A"
     gene            29056..30027
                     /gene="ispB"
                     /locus_tag="ACHOSC_RS00915"
                     /old_locus_tag="pgaptmp_000183"
     CDS             29056..30027
                     /gene="ispB"
                     /locus_tag="ACHOSC_RS00915"
                     /old_locus_tag="pgaptmp_000183"
                     /EC_number="2.5.1.90"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="octaprenyl diphosphate synthase"
                     /protein_id="HGU4546197.1"
                     /translation="MNLEKINELTAQDMAGVNAAILEQLNSDVQLINQLGYYIVSGGG
                     KRIRPMIAVLAARAVGYEGNAHVTIAALIEFIHTATLLHDDVVDESDMRRGKATANAA
                     FGNAASVLVGDFIYTRAFQMMTSLGSLKVLEVMSEAVNVIAEGEVLQLMNVNDPDITE
                     ENYMRVIYSKTARLFEAAAQCSGILAGCTPEEEKGLQDYGRYLGTAFQLIDDLLDYNA
                     DGEQLGKNVGDDLNEGKPTLPLLHAMHHGTPEQAQMIRTAIEQGNGRHLLEPVLEAMN
                     ACGSLEWTRQRAEEEADKAIAALQVLPDTPWREALIGLAHIAVQRDR"
     gene            30254..30532
                     /gene="sfsB"
                     /locus_tag="ACHOSC_RS00920"
                     /old_locus_tag="pgaptmp_000184"
     CDS             30254..30532
                     /gene="sfsB"
                     /locus_tag="ACHOSC_RS00920"
                     /old_locus_tag="pgaptmp_000184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator SfsB"
                     /protein_id="HGU4546198.1"
                     /translation="MESNFIDWHPADIIAGLRKKGTSMAAESRRNGLSSSTLANALSR
                     PWPKGEMIIAKALGTDPWVIWPSRYHDPQTHEFIDRTQLMRSYTKPKK"
     gene            complement(30580..31839)
                     /gene="murA"
                     /locus_tag="ACHOSC_RS00925"
                     /old_locus_tag="pgaptmp_000185"
     CDS             complement(30580..31839)
                     /gene="murA"
                     /locus_tag="ACHOSC_RS00925"
                     /old_locus_tag="pgaptmp_000185"
                     /EC_number="2.5.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008760 - UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase activity [Evidence IEA];
                     GO_process: GO:0019277 - UDP-N-acetylgalactosamine
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HGU4546199.1"
                     /translation="MDKFRVQGPTKLQGEVTISGAKNAALPILFAALLAEEPVEIQNV
                     PKLKDVDTSMKLLSQLGAKVERNGSVHIDARDVNVFCAPYDLVKTMRASIWALGPLVA
                     RFGQGQVSLPGGCTIGARPVDLHISGLEQLGATIKLEEGYVKASVDGRLKGAHIVMDK
                     VSVGATVTIMCAATLAEGTTIIENAAREPEIVDTANFLITLGAKISGQGTDRIVIEGV
                     ERLGGGVYRVLPDRIETGTFLVAAAISRGKIICRNAQPDTLDAVLAKLRDAGADIEVG
                     EDWISLDMHGKRPKAVNVRTAPHPAFPTDMQAQFTLLNLVAEGTGFITETVFENRFMH
                     VPELSRMGAHAEIESNTVICHGVEKLSGAQVMATDLRASASLVLAGCIAEGTTVVDRI
                     YHIDRGYERIEDKLRALGANIERVKGE"
     gene            complement(31894..32148)
                     /gene="ibaG"
                     /locus_tag="ACHOSC_RS00930"
                     /old_locus_tag="pgaptmp_000186"
     CDS             complement(31894..32148)
                     /gene="ibaG"
                     /locus_tag="ACHOSC_RS00930"
                     /old_locus_tag="pgaptmp_000186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417657.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BolA family iron metabolism protein IbaG"
                     /protein_id="HGU4546200.1"
                     /translation="MENNEIQSVLMNALSLQEVHVSGDGSHFQVIAVGELFDGMSRVK
                     KQQTVYGPLMEYIADNRIHAVSIKAYTPAEWARDRKLNGF"
     gene            complement(32308..32601)
                     /gene="mlaB"
                     /locus_tag="ACHOSC_RS00935"
                     /old_locus_tag="pgaptmp_000187"
     CDS             complement(32308..32601)
                     /gene="mlaB"
                     /locus_tag="ACHOSC_RS00935"
                     /old_locus_tag="pgaptmp_000187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312097.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid asymmetry maintenance protein MlaB"
                     /protein_id="HGU4546201.1"
                     /translation="MSESLSWMQTGDTLALSGELDQDVLLPLWEMREEAVKGITCIDL
                     SRVSRVDTGGLALLLHLIDLAKKQGNNVTLQGVNDKVYTLAKLYNLPADVLPR"
     gene            complement(32601..33236)
                     /gene="mlaC"
                     /locus_tag="ACHOSC_RS00940"
                     /old_locus_tag="pgaptmp_000188"
     CDS             complement(32601..33236)
                     /gene="mlaC"
                     /locus_tag="ACHOSC_RS00940"
                     /old_locus_tag="pgaptmp_000188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipid-binding protein MlaC"
                     /protein_id="HGU4546202.1"
                     /translation="MFKRLMMVALLVIAPLSAATAADQTNPYKLMDEAAQKTFDRLKN
                     EQPQIRANPDYLRTIVDQELLPYVQVKYAGALVLGQYYKSATPAQREAYFAAFREYLK
                     QAYGQALAMYHGQTYQIAPEQPLGDKTIVPIRVTIIDPNGRPPVRLDFQWRKNSQTGN
                     WQAYDMIAEGVSMITTKQNEWGTLLRTKGIDGLTAQLKSISQQKITLEEKK"
     gene            complement(33255..33806)
                     /gene="mlaD"
                     /locus_tag="ACHOSC_RS00945"
                     /old_locus_tag="pgaptmp_000189"
     CDS             complement(33255..33806)
                     /gene="mlaD"
                     /locus_tag="ACHOSC_RS00945"
                     /old_locus_tag="pgaptmp_000189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015914 - phospholipid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipid asymmetry maintenance
                     protein MlaD"
                     /protein_id="HGU4546203.1"
                     /translation="MQTKKNEIWVGIFLLAALLAALFVCLKAANVTSIRTEPTYTLYA
                     TFDNIGGLKARSPVSIGGVVVGRVADITLDPKTYLPRVTLEIEQRYNHIPDTSSLSIR
                     TSGLLGEQYLALNVGFEDPELGTAILKDGDTIQDTKSAMVLEDLIGQFLYGSKGDDNK
                     NSGDAPAAAPGNNETTEPVGTTK"
     gene            complement(33811..34593)
                     /gene="mlaE"
                     /locus_tag="ACHOSC_RS00950"
                     /old_locus_tag="pgaptmp_000190"
     CDS             complement(33811..34593)
                     /gene="mlaE"
                     /locus_tag="ACHOSC_RS00950"
                     /old_locus_tag="pgaptmp_000190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014071945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipid asymmetry maintenance ABC transporter
                     permease subunit MlaE"
                     /protein_id="HGU4546204.1"
                     /translation="MLLNALASLGHKGIKTLRTFGRAGLMLFNALVGKPEFRKHAPLL
                     VRQLYNVGVLSMLIIVVSGVFIGMVLGLQGYLVLTTYSAETSLGMLVALSLLRELGPV
                     VAALLFAGRAGSALTAEIGLMRATEQLSSMEMMAVDPLRRVISPRFWAGVISLPLLTV
                     IFVAVGIWGGSLVGVSWKGIDSGFFWSAMQNAVDWRMDLVNCLIKSVVFAITVTWISL
                     FNGYDAIPTSAGISRATTRTVVHSSLAVLGLDFVLTALMFGN"
     gene            complement(34601..35410)
                     /gene="mlaF"
                     /locus_tag="ACHOSC_RS00955"
                     /old_locus_tag="pgaptmp_000191"
     CDS             complement(34601..35410)
                     /gene="mlaF"
                     /locus_tag="ACHOSC_RS00955"
                     /old_locus_tag="pgaptmp_000191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipid ABC transporter ATP-binding protein
                     MlaF"
                     /protein_id="HGU4546205.1"
                     /translation="MEQSVANLVDMRDVSFTRGNRCIFDNISLTVPRGKITAIMGPSG
                     IGKTTLLRLIGGQIAPDHGEILFDGENIPAMSRSRLYTVRKRMSMLFQSGALFTDMNV
                     FDNVAYPLREHTQLPAPLLHSTVMMKLEAVGLRGAAKLMPSELSGGMARRAALARAIA
                     LEPDLIMFDEPFVGQDPITMGVLVKLISELNSALGVTCVVVSHDVPEVLSIADHAWIL
                     ADKKIVAHGSAQALQANPDPRVRQFLDGIADGPVPFRYPAGDYHADLLPGS"
     gene            35620..36597
                     /gene="yrbG"
                     /locus_tag="ACHOSC_RS00960"
                     /old_locus_tag="pgaptmp_000192"
     CDS             35620..36597
                     /gene="yrbG"
                     /locus_tag="ACHOSC_RS00960"
                     /old_locus_tag="pgaptmp_000192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="calcium/sodium antiporter"
                     /protein_id="HGU4546206.1"
                     /translation="MLLATALLIVGLLLVVYSADRLVFAASILCRTFGIPPLIIGMTV
                     VSIGTSLPEVIVSLAASLHEQRDLAVGTALGSNIINILLILGLAALVRPFTVHSDVLR
                     RELPLMLLVSVVAGSVLYDGQLSRSDGIFLLFLAVLWLLFIVKLARQAERQGTDSLTR
                     EQLAELPRDGGLPVAFLWLGIALIIMPVATRMVVDNATVLANYFAISELTMGLTAIAI
                     GTSLPELATAIAGVRKGENDIAVGNIIGANIFNIVIVLGLPALITPGEIDPLAYSRDY
                     SVMLLVSIIFALLCWRRSPQPGRGVGVLLTGGFIVWLAMLYWLSPILVE"
     gene            36611..37597
                     /gene="kdsD"
                     /locus_tag="ACHOSC_RS00965"
                     /old_locus_tag="pgaptmp_000193"
     CDS             36611..37597
                     /gene="kdsD"
                     /locus_tag="ACHOSC_RS00965"
                     /old_locus_tag="pgaptmp_000193"
                     /EC_number="5.3.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA];
                     GO_function: GO:0097367 - carbohydrate derivative binding
                     [Evidence IEA];
                     GO_process: GO:1901135 - carbohydrate derivative metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arabinose-5-phosphate isomerase KdsD"
                     /protein_id="HGU4546207.1"
                     /translation="MSHVELQPGFDFQQAGKEVLAIERECLAELDQYINQNFTLACEK
                     MFWCKGKVVVMGMGKSGHIGRKMAATFASTGTPSFFVHPGEAAHGDLGMVTPQDVVIA
                     ISNSGESSEITALIPVLKRLHVPLICITGRPESSMARAADVHLCVKVAKEACPLGLAP
                     TSSTTATLVMGDALAVALLKARGFTAEDFALSHPGGALGRKLLLRVNDIMHTGDEIPH
                     VKKTASLRDALLEVTRKNLGMTVICDDNMMIEGIFTDGDLRRVFDMGVDVRQLSIADV
                     MTPGGIRVRPGILAVEALNLMQSRHITSVMVADGDHLLGVLHMHDLLRAGVV"
     gene            37618..38184
                     /gene="kdsC"
                     /locus_tag="ACHOSC_RS00970"
                     /old_locus_tag="pgaptmp_000194"
     CDS             37618..38184
                     /gene="kdsC"
                     /locus_tag="ACHOSC_RS00970"
                     /old_locus_tag="pgaptmp_000194"
                     /EC_number="3.1.3.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-manno-octulosonate-8-phosphatase KdsC"
                     /protein_id="HGU4546208.1"
                     /translation="MSKAGASLATCYGPVSADVMAKAENIRLLILDVDGVLSDGLIYM
                     GNNGEELKAFNVRDGYGIRCVLTSDIEVAIITGRKAKLVEDRCATLGITHLYQGQSNK
                     LIAFSDLLEKLAIAPENVAYVGDDLIDWPVMEKVGLSVAVADAHPLLIPRADYVTRIA
                     GGRGAVREVCDLLLLAQGKLDEAKGQSI"
     gene            38181..38756
                     /gene="lptC"
                     /locus_tag="ACHOSC_RS00975"
                     /old_locus_tag="pgaptmp_000195"
     CDS             38181..38756
                     /gene="lptC"
                     /locus_tag="ACHOSC_RS00975"
                     /old_locus_tag="pgaptmp_000195"
                     /EC_number="7.5.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter periplasmic protein
                     LptC"
                     /protein_id="HGU4546209.1"
                     /translation="MSKARRWVIIVLSLAVLVMIGINMAEKDDTAQVVVNNNDPTYKS
                     EHTDTLVYNPEGALSYRLIAQHVEYYSDQAVSWFTQPVLTTFDKDKIPTWSVKADKAK
                     LTNDRMLYLYGHVEVNALVPDSQLRRITTDNAQINLVTQDVTSEDLVTLYGTTFNSSG
                     LKMRGNLRSKNAELIEKVRTSYEIQNKQTQP"
     gene            38725..39282
                     /gene="lptA"
                     /locus_tag="ACHOSC_RS00980"
                     /old_locus_tag="pgaptmp_000196"
     CDS             38725..39282
                     /gene="lptA"
                     /locus_tag="ACHOSC_RS00980"
                     /old_locus_tag="pgaptmp_000196"
                     /EC_number="7.5.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide ABC transporter
                     substrate-binding protein LptA"
                     /protein_id="HGU4546210.1"
                     /translation="MKFKTNKLSLNLVLASSLLAASIPAFAVTGDTDQPIHIESDQQS
                     LDMQGNVVTFTGNVIVTQGTIKINADKVVVTRPGGEQGKEVIDGYGKPATFYQMQDNG
                     KPVEGHASQMHYELAKDFVVLTGNAYLQQVDSNIKGDKITYLVKEQKMQAFSDKGKRV
                     TTVLVPSQLQDKNNKGQTPAQKKGN"
     gene            39289..40014
                     /gene="lptB"
                     /locus_tag="ACHOSC_RS00985"
                     /old_locus_tag="pgaptmp_000197"
     CDS             39289..40014
                     /gene="lptB"
                     /locus_tag="ACHOSC_RS00985"
                     /old_locus_tag="pgaptmp_000197"
                     /EC_number="7.5.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003861862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter ATP-binding protein"
                     /protein_id="HGU4546211.1"
                     /translation="MATLTAKNLAKAYKGRRVVEDVSLTVNSGEIVGLLGPNGAGKTT
                     TFYMVVGIVPRDAGNIIIDDDDISLLPLHARARRGIGYLPQEASIFRRLSVYDNLMAV
                     LQIRDDLSAEQREDRANELMEEFHIEHLRDSMGQSLSGGERRRVEIARALAANPKFIL
                     LDEPFAGVDPISVIDIKRIIEHLRDSGLGVLITDHNVRETLAVCERAYIVSQGHLIAH
                     GTPTEILQDEHVKRVYLGEDFRL"
     gene            40062..41495
                     /gene="rpoN"
                     /locus_tag="ACHOSC_RS00990"
                     /old_locus_tag="pgaptmp_000198"
     CDS             40062..41495
                     /gene="rpoN"
                     /locus_tag="ACHOSC_RS00990"
                     /old_locus_tag="pgaptmp_000198"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0001216 - DNA-binding transcription
                     activator activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase factor sigma-54"
                     /protein_id="HGU4546212.1"
                     /translation="MKQGLQLRLSQQLAMTPQLQQAIRLLQLSTLELQQELQQALESN
                     PLLEQIDTHEEIDTRETQDSETLDTADALEQKEMPEELPLDASWDTIYTAGTPSGTSG
                     DYIDDELPVYQGETTQTLQDYLMWQVELTPFSDTDRAIATSIVDAVDDTGYLTVPLED
                     ILESMGDEEIDIDEVEAVLKRIQRFDPVGVAAKDLRDCLLIQLSQFDKTTPWLEEARL
                     IISDHLDLLANHDFRTLMRVTRLKEDVLKEAVNLIQSLDPRPGQSIQTGEPEYVIPDV
                     LVRKHNGHWTVELNSDSIPRLQINQHYASMCNNARNDGDSQFIRSNLQDAKWLIKSLE
                     SRNDTLLRVSRCIVEQQQAFFEQGEEYMKPMVLADIAQAVEMHESTISRVTTQKYLHS
                     PRGIFELKYFFSSHVNTEGGGEASSTAIRALVKKLIAAENPAKPLSDSKLTSLLSEQG
                     IMVARRTVAKYRESLSIPPSNQRKQLV"
     gene            41518..41805
                     /gene="hpf"
                     /locus_tag="ACHOSC_RS00995"
                     /old_locus_tag="pgaptmp_000199"
     CDS             41518..41805
                     /gene="hpf"
                     /locus_tag="ACHOSC_RS00995"
                     /old_locus_tag="pgaptmp_000199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0044238 - primary metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome hibernation promoting factor"
                     /protein_id="HGU4546213.1"
                     /translation="MQLNITGNNVEITEALREFVTAKFAKLEQYFDRINQVYVVLKVE
                     KVTHTSDATLHVNGGEIHASAEGQDMYAAIDGLIDKLARQLTKHKDKLKQH"
     gene            41923..42414
                     /gene="ptsN"
                     /locus_tag="ACHOSC_RS01000"
                     /old_locus_tag="pgaptmp_000200"
     CDS             41923..42414
                     /gene="ptsN"
                     /locus_tag="ACHOSC_RS01000"
                     /old_locus_tag="pgaptmp_000200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS IIA-like nitrogen regulatory protein PtsN"
                     /protein_id="HGU4546214.1"
                     /translation="MTNNDTTLQLSSVLNRECTRSRVHCQSKKRALEIISELAAKQLS
                     LPPQVVFEAILTREKMGSTGIGNGIAIPHGKLEEDTLRAVGVFVQLETPIAFDAIDNQ
                     PVDLLFALLVPADQTKTHLHTLSLVAKRLADKTICRRLRAAQSDEELYQIITDTEGTP
                     DEA"
     gene            42460..43314
                     /gene="rapZ"
                     /locus_tag="ACHOSC_RS01005"
                     /old_locus_tag="pgaptmp_000201"
     CDS             42460..43314
                     /gene="rapZ"
                     /locus_tag="ACHOSC_RS01005"
                     /old_locus_tag="pgaptmp_000201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNase adapter RapZ"
                     /protein_id="HGU4546215.1"
                     /translation="MVLMIVSGRSGSGKSVALRALEDMGFYCVDNLPVVLLPDLARTL
                     ADREISAAVSIDVRNMPESPEIFEQAMSNLPDAFSPQLLFLDADRNTLIRRYSDTRRL
                     HPLSSKNLSLESAIDKESDLLEPLRSRADLIVDTSEMSVHELAEMLRTRLLGKREREL
                     TMVFESFGFKHGIPIDADYVFDVRFLPNPHWDPKLRPMTGLDKPVAAFLDRHTEVHNF
                     IYQTRSYLELWLPMLETNNRSYLTVAIGCTGGKHRSVYIAEQLADYFRSRGKNVQSRH
                     RTLEKRKP"
     gene            43311..43583
                     /gene="npr"
                     /locus_tag="ACHOSC_RS01010"
                     /old_locus_tag="pgaptmp_000202"
     CDS             43311..43583
                     /gene="npr"
                     /locus_tag="ACHOSC_RS01010"
                     /old_locus_tag="pgaptmp_000202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000216792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS phosphocarrier protein NPr"
                     /protein_id="HGU4546216.1"
                     /translation="MTVKQTVEITNKLGMHARPAMKLFELMQGFDAEVLLRNDEGTEA
                     EANSVIALLMLDSAKGRQIEVEATGPQEEEALAAVIALFNSGFDED"
     gene            43797..44429
                     /gene="yrbL"
                     /locus_tag="ACHOSC_RS01015"
                     /old_locus_tag="pgaptmp_000203"
     CDS             43797..44429
                     /gene="yrbL"
                     /locus_tag="ACHOSC_RS01015"
                     /old_locus_tag="pgaptmp_000203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PhoP regulatory network protein YrbL"
                     /protein_id="HGU4546217.1"
                     /translation="MIRLSEQSPLGTGRHRKCYAHPEDAQRCIKIVYHRGDGGDKEIR
                     RELKYYAHLGRRLKDWSGIPRYHGTVETDCGTGYVYDVIADFDGKPSITLTEFAEQCR
                     YEEDIAQLRQLLKQLKRYLQDNRIVTMSLKPQNILCHRISESEVIPVVCDNIGESTLI
                     PLATWSKWCCLRKQERLWKRFIAQPALAIALQKDLQPRESKTLALTSREA"
     gene            complement(44426..45154)
                     /gene="mtgA"
                     /locus_tag="ACHOSC_RS01020"
                     /old_locus_tag="pgaptmp_000204"
     CDS             complement(44426..45154)
                     /gene="mtgA"
                     /locus_tag="ACHOSC_RS01020"
                     /old_locus_tag="pgaptmp_000204"
                     /EC_number="2.4.99.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009274 - peptidoglycan-based cell wall
                     [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016763 - pentosyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="monofunctional biosynthetic peptidoglycan
                     transglycosylase"
                     /protein_id="HGU4546218.1"
                     /translation="MSKSRLTVFSFVRRFLLRLMVVLAVFWGGGIALFSVAPVPFSAV
                     MVERQVSAWLHGNFRYVAHSDWVSMDQISPWMGLAVIAAEDQKFPEHWGFDVASIEKA
                     LAHNERNENRIRGASTISQQTAKNLFLWDGRSWVRKGLEAGLTLGIETVWSKKRILTV
                     YLNIAEFGDGVFGVEAAAQRYFHKPASKLTRSEAALLAAVLPNPLRFKVSSPSGYVRS
                     RQAWILRQMYQLGGEPFMQQHQLD"
     gene            complement(45151..45804)
                     /gene="elbB"
                     /locus_tag="ACHOSC_RS01025"
                     /old_locus_tag="pgaptmp_000205"
     CDS             complement(45151..45804)
                     /gene="elbB"
                     /locus_tag="ACHOSC_RS01025"
                     /old_locus_tag="pgaptmp_000205"
                     /EC_number="4.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709007.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isoprenoid biosynthesis glyoxalase ElbB"
                     /protein_id="HGU4546219.1"
                     /translation="MKKIGVILSGCGVYDGSEIHEAVLTLLAISRSGAQAVCFAPDKQ
                     QVDVINHLTGEAMTETRNVLIEAARITRGEIRPLAQADAAELDALIVPGGFGAAKNLS
                     NFATLGSECTVDRELKALAQAMHQAGKPLGFMCIAPAMLPKIFDFPLRLTIGTDIDTA
                     EVLEEMGAEHVPCPVDDIVVDEDNKIVTTPAYMLAQNIAEAASGIDKLVSRVLVLAE"
     gene            complement(46034..48370)
                     /gene="arcB"
                     /locus_tag="ACHOSC_RS01030"
                     /old_locus_tag="pgaptmp_000206"
     CDS             complement(46034..48370)
                     /gene="arcB"
                     /locus_tag="ACHOSC_RS01030"
                     /old_locus_tag="pgaptmp_000206"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aerobic respiration two-component sensor
                     histidine kinase ArcB"
                     /protein_id="HGU4546220.1"
                     /translation="MKQIRLLAQYYVDLMMKLGLVRFSMLLALALVVLAIVVQMAVTM
                     VLHGQVESIDVIRSIFFGLLITPWAVYFLSVVVEQLEESRQRLSRLVQKLEEMRERDL
                     SLNVQLKDNIAQLNQEIAVREKAEAELQETFGQLKIEIKEREETQIQLEQQSSFLRSF
                     LDASPDLVFYRNEDKEFSGCNRAMELLTGKSEKQLVHLKPADVYSPEAAAKVIETDEK
                     VFRHNVSLTYEQWLDYPDGRKACFEIRKVPYYDRVGKRHGLMGFGRDITERKRYQDAL
                     ERASRDKTTFISTISHELRTPLNGIVGLSRILLDTELTAEQEKYLKTIHVSAVTLGNI
                     FNDIIDMDKMERRKVQLDNQPVDFTSFLADLENLSALQAQQKGLRFNLEPTLPLPHQV
                     ITDGTRLRQILWNLISNAVKFTQQGQVTVRVRYDEGDMLHFEVEDSGIGIPQDELDKI
                     FAMYYQVKDSHGGKPATGTGIGLAVSRRLAKNMGGDITVTSEQGKGSTFTLTIHAPSV
                     AEEVDDAFDEDDMPLPALNVLLVEDIELNVIVARSVLEKLGNSVDVAMTGKAALEMFK
                     PGEYDLVLLDIQLPDMTGLDISRELTKRYPREDLPPLVALTANVLKDKQEYLNAGMDD
                     VLSKPLSVPALTAMIKKFWDTQDDEESTVTTEENSKSEALLDIPMLEQYLELVGPKLI
                     TDGLAVFEKMMPGYVSVLESNLTAQDKKGIVEEGHKIKGAAGSVGLRHLQQLGQQIQS
                     PDLPAWEDNVGEWIEEMKEEWRHDVEVLKAWVAKATKK"
     gene            complement(48466..49395)
                     /gene="yhcC"
                     /locus_tag="ACHOSC_RS01035"
                     /old_locus_tag="pgaptmp_000207"
     CDS             complement(48466..49395)
                     /gene="yhcC"
                     /locus_tag="ACHOSC_RS01035"
                     /old_locus_tag="pgaptmp_000207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312117.1"
                     /note="This family includes YhcC from E. coli K-12, an
                     uncharacterized radical SAM protein; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01212 family radical SAM protein"
                     /protein_id="HGU4546221.1"
                     /translation="MQLQKLVNMFGGDLTRRYGQKVHKLTLHGGFSCPNRDGTIGRGG
                     CTFCNVASFADEAQQHRSIAEQLAHQANLVNRAKRYLAYFQAYTSTFAEVQVLRSMYQ
                     QAVSQANIVGLCVGTRPDCVPDAVLDLLCEYKDQGYEVWLELGLQTAHDKTLHRINRG
                     HDFACYQRTTQLARQRGLKVCSHLIVGLPGEGQAECLQTLERVVETGVDGIKLHPLHI
                     VKGSIMAKAWEAGRLNGIELEDYTLTAGEMIRHTPPEVIYHRISASARRPTLLAPLWC
                     ENRWTGMVELDRYLNEHGVQGSALERPWIPPTE"
BASE COUNT        12254 a        13483 c        11996 g        12019 t
ORIGIN      
        1 cctgataagc gaagcgcatc aggcattgtg taggcagcag aaatgtcgga taaggcaccg
       61 ctgattactg atacaccggc agtaaattaa agctcgataa aatatggacc agtgcgttgc
      121 caacgccaaa caccagaatc agcgtaatca tcggcttgcc gccccagacg cggaatttcg
      181 ggctgccaaa gcgtttacgc gatgcgcggg ctaacagcgc cggaacaatt gccgcccaga
      241 tggtagctgc taaaccagca taaccaatgg cgtacaggaa tccgttcggg aacagtagac
      301 cacccacaac aggcggggca aaggtcagca atgccgtttt caagcgcccc agggctgagt
      361 catcgaaacc aaacagatct gccagatagt caaacaaacc cagcgttacg ccaaggaacg
      421 aactcgctac cgcaaagttt gagaacacga ccagcagaag gtccaggcta cggctgttca
      481 gcacgccgct taatgcctgt accagcacat caatattacc gcccttctct gcaataccga
      541 taaactccgg acgcgggatg ttacccatcg tcgccagcaa ccagatggta tacagcgcca
      601 gtgccatcag cgtaccgtac accaggcatt tcacgatggt tttcggatct ttgccgtaat
      661 acttcatcag gcttggcacg ttaccgtgat aaccaaacga cgccaggcag aacggcaatg
      721 tcatcagcag atacggcgca taagatgcat tgctttctgc aacgttgaac aatgtcgcag
      781 gctgcacatg ccccaacagg ctgccaaagg tgaggaagaa ggtaatgact ttcgccccca
      841 gcacaatcgc cgtcatgcgg ctgacggcct tagtactcaa ccacaccaca aacgctacca
      901 gcagtgcaaa accaaaaccc gccgcccgtg ccgggacgtt tagcgacatc tctgcgaagg
      961 tgtgatgcag aattgaacca ctggcagaaa tataggcata ggtcaggata tagagcacaa
     1021 aggcaatgga aataccgttg accacgttcc agcctttgcc cagcaaatct ttggtgatgg
     1081 tgtcaaaact cgaaccgatt cggtaattca ggttagcttc cagaatcatc aagccggaat
     1141 gcagcataca gaaccaggta aagatcagcg ccgccattga ccagaaaaac cacgccccgg
     1201 acatgaccac tggcagagaa aacatccctg cgccaataat ggtgccgccg ataatcacca
     1261 cgccgccaag cagcgacggt gacgtttggg tggtggttag tgttgccatg agggcttctc
     1321 tccagtgaaa aatagtgcga ctgcgttgtt atgcattgca ctgtaccagt acacgagtac
     1381 aaaagacaga aaaaaagccc cgatggtaaa aatcggggct gtatatatta ttttacagat
     1441 tgtgttcgct gttcagcgat gattacgcat caccaccgaa acgacgacga ccggtagagt
     1501 catcacgacg cggagcgcgg ccttcacgac gttcgccgct aaaacgacga ccatcaccac
     1561 ggccaccttc acggcgttca ccgctgaagt tacgaccgcc ttcacgacgt tcgccaccga
     1621 aaccacgacc accgccacga cgctcaccgc cagtatgcgg ctgcgcatcg cccagcaact
     1681 gcatattcat cggcttgttc agaatgcgag tacgcgtaaa gtgttgcagt acttcacccg
     1741 gcataccttt cggcagttcg atagtggagt gagaagcaaa cagcttaatg ttaccgatgt
     1801 aacggctgct gatgtcgcct tcgttagcaa tcgcgccaac gatatgacga acttcaacac
     1861 catcatcgcg gcccacttca atgcggtaaa gctgcatgtc accaacatca cgacgttcac
     1921 gacgcggacg atcttcacgg tcgccacgtg ggccacggtc gttacgatcg cgcggaccac
     1981 ggtcatcacg gtcacggaat tcacgtttcg gacgcatcgg cgcatctggc ggaacaatca
     2041 gagtacgttc accctgtgcc attttcagca gtgccgcagc cagagtttcg agatcctgct
     2101 cttcaccttc tgcagtcggc tgaattttgc tcagcagtgc gcggtattga tccagatcgc
     2161 tgctttccag ctgctgctgt actttagcgg cgaatttttc cagacggcgt ttgcccagca
     2221 gttctgcgtt cggcagttct acttccggaa tagtcagctt catagtacgt tcaatgttgc
     2281 gcagcagacg acgctcgcgg ttctcaacga acagcagcgc gcggccagca cgacccgcac
     2341 gaccggtacg accgatacgg tgaacgtaag actcagaatc catcgggata tcgtagttaa
     2401 ctaccaggct gatacgctca acgtccaggc cacgggctgc aacgtcggtc gcaatcagga
     2461 tgtccagacg accatctttc aggcgttcca gtgtctgttc acgcagcgcc tggttcatgt
     2521 caccgttcag cgcggcgctg ttgtagccgt tacgctcaag agcttcagcc acttccagag
     2581 tcgcgttttt ggtacgaacg aagataatcg ccgcatcaaa atcttccgct tccaggaaac
     2641 gtaccagtgc ttcgtttttg cgcatacccc agacagtcca gtagctctgg ctgatgtcag
     2701 gacgggtagt cacgctggac tgaatgcgca cttcctgcgg ctctttcata aagcggcggg
     2761 taatgcgacg aatcgcttcc ggcatggttg cagagaacag agcggtctga tgaccttccg
     2821 ggatctgcgc cataatggtt tcaacgtctt cgatgaagcc catgcgcagc atttcgtcag
     2881 cttcatccag aaccagaccg ctcagtttag agaggtccag agtgccacgt ttcaggtggt
     2941 ccagcagacg gcccggagta ccgacaacga tctgcggccc ctgacgcagg gcgcgtaatt
     3001 gcacgtcata acgctggccg ccgtatagag caaccacatt tacgccgcgc atgtgtttag
     3061 agaaatccgt cattgcttca gcaacctgta ccgccagttc gcgggtcggt gccagcacca
     3121 gaatctgtgg tgctttcagc tcaggatcaa gattctgcaa cagaggtaaa gagaatgctg
     3181 cagtttttcc gctccccgtc tgggccatac ccagaacgtc gcggccattc agcagatgtg
     3241 gaatacactc tgcctgaatt ggagatggtt tttcgtaacc cagatcgtta agggcttcaa
     3301 ggataggagc cttcaggccc agatctgcaa aagtggtttc gaattcagcc atgtagtacg
     3361 tgtgcctcaa aattaatggc ggccagtcta cataactcat catgaaattg atcagcaatt
     3421 ttcattgaaa agtgtgaacc ggctcaaagt aggtgtatta acgaacaaca acgccctcac
     3481 ccgttaaggt gatggcaatc aaaaaagatt acgggctgat gtgtacgtca gctattgctg
     3541 gtccgattct gccaggtcat cttggtcctg gcccaggagc gataattcca acaatgcgta
     3601 tcggtgctca acaaagttat gaacgttgtt ggcaaccgcc agtttgaaca gtgccgtggc
     3661 gctgtccaaa tcccccagac ttaggtagta cttacctaaa tagaagttgg tttcactgag
     3721 atgctcagcg agcgaggtgt tatccgttgc gtccgccttg agcctttcca ttaacgtttg
     3781 ttcgctaatg ttgcccaggt agaactcgac aatgttccat ccccactgtt ccttatccga
     3841 tttttcgaag tgctgtttca acacttcttt agcctgcttc tcatcgagct tctgctcggc
     3901 gagataaagc cacagactac ggaaaggatc attgggatcg tcttgataaa acgccagcag
     3961 atcatcttgc gctaacttgt cacgaccgcc gtaatataat gcgatcccgc gattcaagtg
     4021 cgcgtagttg taagttggat caagctcaag tacagaatca aacgcttcat aggcagcatc
     4081 aaaattgcct gcctgcgtta aatatatgcc taagtaattg aatacttcag gcatatccgg
     4141 tcggattgcc agcgcttgcg aaaaatcgtt acgcgctaat gccctcagac cgagactatc
     4201 atacaacact ccgcgctcat ataaaagctg tgcgcgttcg tcatcggtta aagcccgact
     4261 ggcaaggatt tgttccatac gtgccagaat cacttcctgc tgtaaagtcg gttgcaatgg
     4321 taccgcgagg acttcacttt tacgccagga agtattactg catcctgcaa gcgtaagtgc
     4381 tgtcgcaacg aaacaccagc gcaaaaaagg cttcatttcc cactcccgaa gaccacggtt
     4441 gaatgaacgt cctgttcccg gttgctaaca aggcgtcctg cccggttaaa agccccccgc
     4501 cgcagcggag ggcaaatggc aaccttactc gccctgttca gcagccggag cttccggtgc
     4561 tgcagcaggt tgagactgct cagtcgcttc tttaatgctc agacggatac ggccctggcg
     4621 atcaacttcc agaactttca ccggtacttc ctgacccatc tgcaggtaat cggtcacttt
     4681 ctcaacgcgt ttgtcagcga tttgagagat gtggaccaga ccttctttac cgccgccgat
     4741 ggcaacaaat gcgccaaagt caacgatacg ggtcacttta ccagtgtaga cgcggcccac
     4801 ttcgatttct gcagtgatct cttcgatacg acgaatagca tgtttcgctt tctcgccgtc
     4861 ggtcgctgcg atcttcacag taccgtcatc ttcgatttcg atggtagtgc cagtttcttc
     4921 ggtcagggca cggataacag agccgccttt accgataacg tctttgatct tgtccgggtt
     4981 gatcttgatg gtatggatac gcggtgcgaa ctcagagata tcgccacgcg gcgcgttgat
     5041 cgcctgttcc attacgccca ggatatgcag acgcgcacct ttagcctggt tcagcgcaac
     5101 ctgcatgatc tctttggtga taccttcaat tttgatatcc atctgcaatg cagagatacc
     5161 ctcgcgggaa cccgcaactt tgaagtccat atcgcccagg tgatcttcgt cgcccaaaat
     5221 gtcagacagt acaacgtagt tgtcgccttc tttcaccaga cccattgcga tacccgcaac
     5281 ggcagctttg atcggcacac ctgcgtccat cagcgccaga gacgcgccgc acacggaagc
     5341 catagaagag gaaccgttgg attcagtgat ttcagacaca acacgtacgg tgtacgggaa
     5401 tttgtccata tccggcatga ctgccagcac gccgcgcttc gccagacgac cgtgaccaat
     5461 ttcacgacgc ttcggagaac cgaccatgcc ggtttcgcct acggagtacg gagggaagtt
     5521 gtagtggaac aggaaggtat cggtacgttc gcccatcagt tcatcaagaa cctgcgcgtc
     5581 acgagcagta cccagcgttg cggtaaccag cgcctgcgtt tcaccacggg tgaacagcgc
     5641 agaaccgtga gtacgcggca gcacgccagt acgcacatcc agaccacgga tcatatcttt
     5701 ttcacgaccg tcgatacgcg gttcgcctgc cagtacgcgg ctacgaacaa cgtttttctc
     5761 gatagcgtgc agaatttcac ccagttcgtt ttcgtccagg gtttcgtctt cagcaagcag
     5821 cgtcgcgatg gtttcagatt tgatgacatc aacctgcgca taacgctctt gtttgtcggt
     5881 gatgcggtaa gcatcgctca ggcgagcttc agccagtgca gcaacgcgcg cgttcagcgc
     5941 ttcgtttacc ggctccggct gccagtccca acgtggttta ccggcttctt tcaccagttc
     6001 attgatgttc tgaataacaa cctgctgttg ttcatgaccg aacactactg cgcccagcat
     6061 ctggtcttcg ctcagcagtt cagcttcaga ttcaaccatc agaacagcgg cttcagtacc
     6121 ggcaacaacc agatccagtt tgctctcttt cagctcgtcc tgagtcgggt tcagtacgta
     6181 ctggtcattg atgtaaccta cgcgagcagc accaatcgga ccattgaacg gaataccaga
     6241 cagagacagt gctgcggaag caccaatcat cgcgacgata tccgggttaa cttgcgggtt
     6301 aacagaaacc acggtggcga taacctgaac ttcgttgacg aagccttccg ggaacagcgg
     6361 gcgaatcggg cggtcaatca gacgcgcgat cagggtttcg ccttcgcttg ggcggccttc
     6421 acgacggaag aagctacccg ggatacgacc agcagcgtag gtacgctcct gatagttaac
     6481 ggtcagtggg aagaagtcct gacctggttt ggcttttttc tggccaacaa cggtaacaaa
     6541 taccgcggtg tcatccatgc taaccataac agcggcagta gcctgacgag ccatcatgcc
     6601 ggtttccaga gtcacggtgt gttggccgta ctggaattta cgaacgatcg gattaagcaa
     6661 tgtaatatcc tttctctttc ttagacagta ccttacggca ctggtgttaa tacccgatct
     6721 tctgcgcatc ctcgcgacta atgacaaccc taacccagct ctatgtgggt aaagcctctc
     6781 attagccgcg cgaacctctg caacggaaga tcattcatag caacaataca ttagtttcca
     6841 gtgaattgct gccgtcagct tgaaaaaagg ggccactcag gccccctttt ctgaaactcg
     6901 caagaattag cgacgcagac ccaggcgctc gatgagctgg gtgtaacgtg ctacgtcttt
     6961 acgtttcagg tagtcgagca gtttacgacg ctgagaaacc atgcgcagca gaccacgacg
     7021 gctgtggtga tcttttttgt gctctgcaaa gtggccctgc aggtggttga tctgtgcagt
     7081 cagcagtgct acctgaactt cggtagaacc ggtgtcgttt gcgtcacgac caaactcaga
     7141 aacgatttta gctgttgctt cagtacttag agacatttta aaactccaaa gtatatagaa
     7201 tgaaaggacg ccgatctcta attcagcgat cccagtgtac gttacgcaaa gtgttaaaca
     7261 atttacgcga cgttaagcgg cagtattcta ctcgtagcga cctgttatcg caagacggtt
     7321 aacattacgc cgggtattca accaccaggc gacgaggcgc aacgcggcct tcatcgtcaa
     7381 tttcgcccat accgataaat ttgccgttct caccttctgt aacgcgaacc agtccttcca
     7441 gcggcgcacc ggatgtacga actgggttac catttttgaa gtaaacagaa gacgttaacg
     7501 gaagattcac caccgggtag tccgaagctg gactgtccat tggcatcagt aatggatcaa
     7561 gtaactccgc agccggaata tcctgctgtt cggcttgttc gaccagttca cgcagatgtt
     7621 ccagggtcac catccgttca accggatatt tacttaccgc cagacgacgc aggtaaataa
     7681 catgcgcgcc acaaccgagt ttttcaccca ggtcatcaat gatggtgcgg atataagtgc
     7741 cttttgagca gtgaatttcc agctccagct cattgccttc gtggcgaata aacagcaatt
     7801 cataaacggt aatcgggcgc gcttcacgcg gtacttcaat gccctgacgc gcatattcgt
     7861 acagtttttt accctgatat ttcagtgctg aatacatcga agggatctgt tcgatatcgc
     7921 cacggaaagt atccagcgcc gccgccagtt gctctgcgct aaaggttacc ggacgttctt
     7981 caacgatttg tccgtcggca tcagaagtat cggtacgctg tccaagacgc gcaatgaccc
     8041 gatagcgttt gtcggagtcc agcagatact gggaaaactt cgtcgcttcc ccgaggcaaa
     8101 ttggcaacat gccagttgcc agcgggtcca gcgcaccggt atgcccggca cggttggcgt
     8161 tataaatccg tttcactttt tgcagcgcat cattgctgga cataccctgc gatttatcca
     8221 gcaacaaaac gccgttaatg tcgcgaccgc gacgacgagg acgactcatt agtcctcctt
     8281 gctgtcgtcc gggttaacac gacgttcttc gtcatgtttg accacgctgg tcaccaggtt
     8341 tgacatgcgc atcccttcaa ccagagagtt gtcgtagaag aaggtcagtt ccggcacgat
     8401 acgcaggcgc atcgctttcc ccagcaggct gcggatgaaa ccagaagctt cttgcaacgc
     8461 tttgatgccc gctttaaccg cgtcttcatc tttgtcgttg aggaacgtca catatacttt
     8521 ggcatacgcc aggtcgcgag acatttcgac accggaaacg gtggtcatca tgcccaggcg
     8581 aggatcttta atttcacgct gcaggatgag agcgatctct ttttgcattt cctgcgctac
     8641 gcgctgcggg cgaccaaatt ctttcgccat aataaattct cctgacaaaa aaggggctgt
     8701 tagccccttt ttaaaattaa tttcaggtgg aagggctgtt cacgttggcc tgataagacg
     8761 cgccagcgtc acatcaggca atccatgccg gatgcggcat aaacgcctta tcccgcatgg
     8821 aaccctaaaa accttaagcg atggtacgtt ggatctcgat gatttcgaat acttcgatca
     8881 catcgccagt gcggacgtcg ttgtagttct taacgccgat accacattcc ataccgttac
     8941 ggacttcgtt aacgtcatct ttgaagcggc gcagggactc cagctcgcct tcgtagataa
     9001 ccacgttgtc acgcagaacg cggatcgggt tgtgacgttt aaccacacct tcggtaacca
     9061 tacagcctgc gatggcacca aatttcggcg atttgaacac gtcacgaact tccgccagac
     9121 cgataatctg ctgtttcagt tccggagaca gcataccgct catcgccgct ttcacttcgt
     9181 caatcaggtt atagatgacg gagtagtaac gcagatccag gctttccgct tcaatcactt
     9241 tacgtgcaga ggcatcagca cgtacgttga agccaaccag gatggcgttg gacgccgcag
     9301 ccagggtggc gtcggtttcg gtgataccac ctacgccaga accgatgatc ttcactttaa
     9361 cttcgtcagt agacagtttc agcaaggagt cggagatcgc ttccacagaa ccctgtacgt
     9421 ctgccttcag gacgatgttc acttcgtgaa cttcgccttc ggtcatgttg gcgaacatgt
     9481 tctcgagttt agatttctgc tgacgcgcca gtttaacttc gcggaattta ccctgacgat
     9541 acagtgcaac ttcacgcgct ttcttctcgt cacgtacaac ggtaacttca tcacccgcag
     9601 ccggtacgcc ggacaggccg aggatttcca ccggaatgga cggacccgct tccagcactt
     9661 cctgacccag ttcgttacgc atcgcacgaa cacgaccgta ttcgaagcca cacagaacga
     9721 tatcgccctt gtgcagagta ccttcacgta ccagaacggt agcaaccgga ccacgacctt
     9781 tatcgaggaa ggattcgata accgcaccgc tcgccatacc tttacgtacc gctttcagct
     9841 ccagaacttc cgcctgcagc aggatagcgt ccagcagttc atcgatgccg gtacccgctt
     9901 tcgcagatac gtgtacgaac tggctttcac cgccccactc ttccggcagg atgccatact
     9961 gggagagttc gttcttaaca cgatccggat cggcttctgg tttatcaatc ttgttcactg
    10021 caaccaccac cggtacctgc gccgctttcg cgtgctggat tgcttcgatg gtctgcggca
    10081 tcacgccgtc gtcggcagca acaaccagga ctacgatgtc cgttgcctgc gcaccacgtg
    10141 cacgcattga agtaaacgcg gcgtgccccg gggtgtccag gaaggtgatc atgccgtttt
    10201 cagtttcaac gtggtatgca ccaatgtgct gggtaatgcc gcccgcttcg ccagaggcaa
    10261 ctttcgttga acgaatgtag tccagcagag aggttttacc gtggtcaacg tgacccatga
    10321 tggtcacaac cggcgcgcgc ggttcagccg cagcacccgt gtcacggtcg ctcattaccg
    10381 cctcttccag ctcgttttca cgacgcagga taactttgtg gcccatctct tcagcaacca
    10441 gctgtgcggt ttcctgatcg ataacctggt tgatggttgc cattgcgccc agtttcatca
    10501 tcgctttgat gacctgagag cctttaaccg ccatcttgtt cgccagttcg ccaacggtga
    10561 tggtttcgcc gatcacaacg tcacggttaa cggcctgagc tggcttctgg aagccttgct
    10621 gcagggaaga acctttacgt tttccgcctt taccgccacg tactgctgcg cgtgcttctt
    10681 cacgatcagc ttttgattca gcgtgtttgt tgcctttctt cggacgcgct gctttcgcgt
    10741 tacgaccacg gccacgaccg ccttcgactt cacgatcgct ttcgtcttct gcctggcgag
    10801 catgttgaga agtagtgacg tgataatcgc tggaatcttc agtcggttcc gcgttatcag
    10861 tccatttgtt ttcttccgcc atacgacgtg cttcttcagc aacgcgacgt gcttcttctt
    10921 cgagtttacg acgcgcttct tcttcagctt tacgcttgag ctctgcagct tcctgctcac
    10981 ggcgggcttt ttcagcctgg gcgtttttag tcatatcgtc ttgttgattg ctcactttgt
    11041 ctttttccgc agcttcacgt ttcgcttgtt cagcagcttc acgcttagct tgttctgcgg
    11101 cctcacgttc agctttttgt tgcgcctcgc gtttagccga ttcttctgcc tcacgacggg
    11161 cttgctcttc cgcttcacgc tgcgcttgct cttccgctgc aaggcgttca gcctcttgcg
    11221 gatcgcgttt cacaaaggtg cgtttcttgc ggacttcgat ttgtaccgat ttgctttttc
    11281 caccggtacc aggaatgtta agggtgctgc gtgttttacg ttgcagcgtc aatttgtccg
    11341 ggcctgaatt tttctgattc aggtggtcaa tcaaagtctg tttctcttgt gcagacacag
    11401 agtcgtcagc agacttccgg atacctgcat cagcaaattg ctgtaccagg cgttccacgg
    11461 aggtctgtcg ctctgcggcc agcgttttaa tcgttacatc tgtcatgctg ttccttcctg
    11521 ctacagttta ttacgcttcg tcaccgaacc agcaaatatt acgggcagcc ataatcagtg
    11581 ctccggcttt ttcgtcggtc aacccttcga tatcagccag atcatcaatg ccctgttcgg
    11641 cgagatcttc cagcgtacaa acgccacggg cggccagttt gaatgccaaa tcacgatcta
    11701 tcccttcaag gttcagcaga tcgtcagccg gtttgttatc accgaggctt tcttcctggg
    11761 cctgtgcaat ggtggccagt gcatttttag cacgctcgcg cagtgcttca acggtcggct
    11821 catcaaggcc ttcgatttcc aacagctctt tcatcggcac ataggccagt tcttccagcg
    11881 tcgagaagcc ttcttctacc agaacagtcg cgaagtcttc gtcgatgtcg agatatttgg
    11941 tgaaggtgtc gatcgctgcg tgcgcttccg cctgatgctt agcctgcagg tcgtcaacgg
    12001 tcatcacgtt gagttcccaa ccgctcagct gcgaagccag acgcacgttc tgaccgttac
    12061 ggccaatcgc ctgcgccagg ttaccggctt caacggcgat atccatggtg tgtttatctt
    12121 catccaccac gatagaagca acgtctgccg gtgccattgc gttaatcaca aactgcgccg
    12181 ggttatcatc ccacaggacg atatcgatac gctcgccgcc cagttcagta gaaaccgcct
    12241 gaacacgcgc gccacgcata cctacacagg cacctaccgg atcgatacgt ttatcgttgg
    12301 ttttcaccgc gattttcgca cgagaacccg gatcgcgagc cgctgcttta atttcaatca
    12361 cttcttcgcc gatttctggc acttcaatac ggaacagttc gatcagcatt tccggcttgg
    12421 aacgagtgac gaacagttgc gcgccacgcg cttccgggcg aacggaatag agcacgccac
    12481 gaacgcggtc gccagggcgg aagttttcac gcggcagcat atcttcgcgc aggatcacgg
    12541 cttcagcatt gttgcccaga tccagagaga tgttgtcgcg gtttactttt ttcaccacgc
    12601 cggtgatgat ttcaccttcg tgttcacgga actgatcaac caccatcgca cgttcggctt
    12661 cacgcacttt ctgcacgata acctgttttg ccgtctgggt agtgatacgg tcaaaggtaa
    12721 cagactcaat ctgatcttca acgtaatcgc ccaggttcag gctttcatct tcataacgtg
    12781 cggcttcaag ggtgatttcc ttggtcggct gggtgacttc atcaacaact aaccagcgac
    12841 ggaaggtgtc aaaatcaccg cttttgcgat cgatctgtac gcggacgtcg atctcttgtt
    12901 catatttttt ctttgttgct gtcgccagcg cgctttccaa tgcttcgaaa atcttctcgc
    12961 gaggtagcgc cttttcattg gatacggctt caactacagc caaaatttct ttgttcatcg
    13021 cgggcttttc acctcatcca gactattaaa agtggggaac caggttcgcc ttctggatat
    13081 tactcagcgc gaacacttca tctttacctt cgacggtaac tgtgatcatt tcaccgtcta
    13141 ccgctttgat aacgccctgc catttacgac ggttttgtac cgccatacgg agaaccagag
    13201 tcacctcttc tccgacaaaa cgggcgtagt gttcagccgt gaacagtggg cgatcgagac
    13261 ccggtgagga gacttccagg ttataagcaa cggtgatggg atcttcaaca tccagcacag
    13321 cacttacctg gtggctcaca tcagcacaat catcaacatt gatgccatct tcactatcaa
    13381 tatagatgcg cagtgtggat gtgcgaccgc gaataaattc gatgccaacc agttcaaaac
    13441 ccagggcctc aactggcgca gtaatcatct ctgttaattt ttgctctaat gtggacaagc
    13501 ccacccccaa gacataaaaa aagggcctaa agcccagtta ttctgtagtc agataacaaa
    13561 aaaccccgat aaatcggggc tttatataac tgaaccctat aaccgcaact gcggtctgga
    13621 gcactttcca gaaggatttt ttcaaatccc actacgaagg ccgaagtctt cacagtatat
    13681 ttgaaaaagg actctaaggg aaagtggttg cgggggccgg atttgaaccg acgaccttcg
    13741 ggttatgagc ccgacgagct accaggctgc tccaccccgc gcctgaaacg tggcaaattc
    13801 tactcgtttt gggtaaaaga tgcaaatact gctgggattt ggtgtaccga gacgggacgt
    13861 aaaatctgca ggcattatag tgatccatgc cacattttgt caacgtttat tgctaataac
    13921 gtgaatgaat atccagttca ctttcatttg ttgaatactt ttgccttctc ctgctctccc
    13981 ttaagcgcat tattttacaa aaaacacact aaactcttcc tgtctccgat aaaagatgat
    14041 taaatgaaaa ctcatttatt ttgcataaaa attcagtgag agcggaaatc caggctcatc
    14101 atcagttaat taagcagggt gttattttat gacgacgatt ctcaagcatc tcccggtagg
    14161 tcaacgtatt ggtatcgctt tttccggcgg tctggacacc agtgccgcac tgctgtggat
    14221 gcgacaaaag ggagcggttc cttatgcata tactgcaaac ctgggccagc cagacgaaga
    14281 ggattatgat gcgatccctc gtcgtgccat ggaatacggc gcggagaacg cacgtctgat
    14341 cgactgccgc aaacaactgg tggccgaagg tattgccgct attcagtgtg gcgcatttca
    14401 taacaccacc ggcggcctga cctatttcaa cacgacgccg ctgggccgcg ccgtgactgg
    14461 taccatgctg gttgctgcga tgaaagaaga tggcgtgaat atctggggtg acggtagcac
    14521 ctataaagga aacgatatcg aacgtttcta tcgttatggt ctgctgacca atgctgaact
    14581 gcagatttac aaaccgtggc ttgatactga ctttattgat gaactgggcg gccgtcatga
    14641 gatgtctgaa tttatgattg cctgcggttt cgactacaaa atgtctgtcg aaaaagccta
    14701 ctccacagac tccaacatgc ttggtgcaac gcatgaagcg aaggatctgg aatacctcaa
    14761 ctccagcgtc aaaatcgtca acccgattat gggcgtgaaa ttctgggatg agagcgtgaa
    14821 gatcccggca gaagaagtca cagtacgctt tgagcaaggt catccggtgg cgctgaacgg
    14881 taaaaccttt agcgacgacg tagaaatgat gctggaagct aaccgcatcg gcggtcgtca
    14941 cggcctgggc atgagcgacc agattgagaa ccgtatcatc gaagcgaaaa gccgtggtat
    15001 ttacgaagct ccggggatgg cactgctgca cattgcgtat gaacgtctgt tgaccggtat
    15061 tcacaacgaa gacaccattg agcagtatca cgcgcatggt cgtcagttgg gccgtctgct
    15121 gtaccagggg cgttggtttg actcccaggc gctgatgctg cgtgactctc tgcaacgctg
    15181 ggttgccagc cagatcactg gtgaagttac cctggagctg cgccgtggga acgattattc
    15241 aatcctgaat accgtctcag agaacctgac ctacaagcca gagcgtctga cgatggaaaa
    15301 aggcgactcg gtgttctcgc cagatgatcg tattggtcaa ttgaccatgc gtaatctgga
    15361 tatcactgat acccgagaga aactttttgg ttatgccaaa actggcctgc tctcctcctc
    15421 tgccacttca ggcgtgccgc aggtggagaa tatggaaaac aaaggccagt aattcgacat
    15481 caaccctgcc cttcaggggt ggggttacct tgcttatcct ctgtaaactc ctttctgagc
    15541 atcacataat cgaaaacatc atggtttgca tccacaaccc gtgagtctcc tttatagtgt
    15601 gcaatcactt cctcaagcgt ttgcggctgt tccggctttg ttacccccat ccacgcatta
    15661 ataaggtaat tattgtaagc tgtcgaaaag atgttgtttt ccgttgtgcg atccacgcca
    15721 tccccaagaa cggggctata ccagataaac atcgggacat gatatgcctg ctggctagcc
    15781 tccctgcctc catgaaaata gacgttcttc ttcgtagggt cacgttccag gccgtggtcg
    15841 gcaaaataca tgaccgaggc gcggcgatct tttaataatt caaaaacctg acctagcaaa
    15901 ctatctgtgt aatgaatgga gttgtcatag caggcatcct gatcgtcctg cggttgaaac
    15961 acggcgctgg attgcggata ggcgctacaa gccggttcat ggcttccatt taaatgaaga
    16021 acaatcagtt tcttttgctg cgtattttgc tgtaatgctt gcgataaatg cggcaacaac
    16081 aattcatcaa atcctctgac ataaactgtt tccatggcgc gcatggcgat actggtaact
    16141 gctgtaccat tctgccgaaa agcggattgc gagcttagcc agaaagtctg aaatcctgct
    16201 tgattagcca tattaataat gttgtcgggg taattatgaa tgtcatgact caaaacagaa
    16261 tcagcagtta aagaaagggg aaccgacagc gcagtgtaag gtgcgccgct tattgcttga
    16321 ttaaacagtt tgatctgttt tctttgtgct tcaacttgcg gtgtcgtaga gcgtgtatat
    16381 ccatacaaag acatattgtc gacacgtaca gactccccca caatcaacac gtaggtatca
    16441 atacctgtat ccctgactga taattgaaaa tacggcaccg tgtttgcaat tgagagtaat
    16501 ctttgatgct ctttcgctgc taaagcaaaa tagttgagat tgaaaaacgg cgtataggta
    16561 gcaaatcgcg acgctagtat atatggactg aacgcttttt tattttttgc atctttataa
    16621 gcaaattgac aagcggaaaa taaactgccc gaaatgacaa tcagcaataa tattcctgtc
    16681 actttttttg tcggcaagga aacatcatat tttattatta ctgccaaaaa aagaagggat
    16741 aaaaaagcaa aggcacatag ataagggata tacatcccca gcattttgac aacttcatcc
    16801 ggatcactct ggagcacact aatcgcaaat ccatcattga aagttgtacc aaaggtacac
    16861 catgaataga ggctaatact catgtctgcc gcagtcagta acacaaatgg tgcggcaaca
    16921 agcaggcgaa gcggaaggga tgatatacgt tttacaacca ggatggtcag gacgaagaac
    16981 agcaatcgct gaaccatatg cgcgccggaa gaggcgacta agttcgtaat accaaaaaca
    17041 ataacacaaa gatacaacaa ccaatgagat ttaaaacttc tagcaaattt gttgaatact
    17101 gtcatccaga aatccaagtt cacaaccaat ataaacaatt atcaacaatg ttcaatttta
    17161 tatcagacat gtttttcaac tctgatccat atcagaccga aacactgtta gttttttctt
    17221 tttaaatgat gcggccattg cgttctcagt aattaaataa cattatcaat gcgtctctaa
    17281 acattacgca gaacgtagcc atcagtagtt atactgacag gagcaggatg gtttatcgag
    17341 aagtaaacat atccgtattc gtaccggaac tggagttgac acaataaagt gccaattatg
    17401 tcagcagaag ggaaaagttt ataactaagg cgtaccggca ccatcgtttc aaggtaccag
    17461 ctacgagtaa agcaactgga cgagatacag atacctgaca accattcctc agaccaggac
    17521 caaaacgaaa aaagacgctt ttcagcgtct cttttctgga atttggtacc gaggacggga
    17581 cttgaacccg taagccctat tgggcactac cacctcaagg tagcgtgtct accaattcca
    17641 ccacctcggc acggatacta ctttttagtt cgggatatcg ctggtcggct tagccggagc
    17701 agctggttga gtttgttcgg ttttcaccgg tgcactcaga ttttcccatt cgctaccttt
    17761 attggttttg ttgctattga tgttacccag caccagactg atgatgaaga ataacgttgc
    17821 cagcagcgcc gtcatgcggg tcatgaagtt accagaacca cttgaaccaa acagcgtagc
    17881 ggaagcgcct gctccgaagg aggctcccat atcagcgcct ttaccttgct gcagcatgat
    17941 cagaccaaca aggccaattg ccacaataag gaaaactact aaaagagctt cgtacataat
    18001 caacctgttc cttgcggagt tgccgcgtac caatgcttca accaataaag cgggagtttt
    18061 taatctttcc cactgaagcg ggtgtgaata ctaaccaaag cgaatgacct tcgcaagggc
    18121 aattttatcg cattgtatca actgcggaaa aaaacagcaa aaccgatgtg ttcgctgaga
    18181 aaaaaggcga caatcgccgc ctttttagcc agttatctaa cgctttaaac ggcttttact
    18241 gcatcggcga tgcggtgtgc aaattcagtc acctgcgctt cgtcttcgcc ttccaccatc
    18301 acgcgaatta acggttcggt gccggattta cgcagcaaca cgcgtccacg gttgcccagc
    18361 gcagcttcaa cctctgcggt cacggcttta actgactcat gctcaagtgg atcgccgcta
    18421 cctgcggtgt aacgtacgtt aaccagaatc tgcgggaaca ttttcatgcc gctgcaaagg
    18481 tcgtgcaggc tcatatggtt acgtgccatc gccgccagca cctgcaagcc agcaacgatg
    18541 ccgtcaccgg tagtagtttt atccagtagg atcacatgac cggaattctc tgcaccgata
    18601 cgccagcctt tctcctgcat tttttccagt acgtagcggt cgcccacttt cgcgcgcgca
    18661 aatggaattc ctaactgttt cagcgccagt tcaagcccca tgttgctcat caatgtaccc
    18721 acagcgccac cacgcagctg gccctgacga agaccttcac gcgcgatgat atacatgatc
    18781 tgatcgccat cgactttatt gccttcatgg tcaaccataa tcacgcgatc gccatcgccg
    18841 tcgaaggcaa taccgagatc cgctttttca gccagcacac gagcctggag cgcgcgaacg
    18901 tcggtagccc ccacttcggc attgatgttt acaccgtttg gctcacaacc gatagcgata
    18961 acgttcgccc ccagttcgcg cagcacgttc ggcgcgatgt gataagtcgc accgtttgca
    19021 caatccacca caatcttcag ttcactgagg ctaagttcgt tcgggaacgt ggctttgcaa
    19081 aactcgatat agcgacccgc ggcatcaacg atacggctgg ctttacccag ttctgccgaa
    19141 tcaacgcagc tgatctcctt ttccatttcc gcttcgatgg cctcttctac cgcatccggc
    19201 agtttggtgc cgtcgataga gaagaattta atgccattat cgtagaacgg gttatgagat
    19261 gcagatatca caattccggc ctctgcgcgg aaggtacgcg tcagataagc cacggccggt
    19321 gttggcatcg ggccagtgaa gagtgcggaa aggcccgctg ccgccagacc cgcttccagt
    19381 gctgactcca gcatatagcc agaaatacgc gtgtctttac caataataat cttacgggag
    19441 ccgtggcgcg ccagcacttt acccgcggcc caacccagct taagcacaaa atcaggtgtg
    19501 atcggcgcat cccctacacg accacgaatc ccatcggtac cgaaatattt acgattactc
    19561 atagcgtttg ttttcctttg cagacagagt ggcttccacc acccgcatcg cttctacggt
    19621 ttctttgacg tcatgaacac gaatgatgtg cgcgccttgc attgcggcaa tgaccgcaca
    19681 ggccagacta ccgctcaggc gctcggacgg ccccacgttc agcagctgcc caatcatcga
    19741 ttttcgtgac atacccacca acagcggcag gttgaaatgg tgaaattcag ccaggcgcgc
    19801 cagtaatgaa tagttatggg agagattttt accgaaaccg aatccggggt cgagcaacaa
    19861 tttctctttt gcgatacccg cctgctcgca acgtgctatt tgctcaataa agtagcgatt
    19921 cacttctgca aagacatcgt catacttcgg agcttcctgc atggtttttg gatttccctg
    19981 catatgcatc agacaaaccg gtaaaccggt ttctgcagcc gcctccagag cgccaggttc
    20041 ggaaagggag cggatatcat taataatgtg agcgccaact ttcgctgact cacggatgac
    20101 ttctggtttg gatgtatcga ccgagatcca gacttcgaag cgttgagcaa ttgcctcaac
    20161 cacaggaata acacgttgca actcttcttc aacgctaact tccgccgccc ctgggcgcgt
    20221 ggactcgcca ccaacgtcaa tgatcgtcgc gccagcattg atcatcagat tcgcatgttt
    20281 caccgcatct atcagcgagt tatgcgtgcc accatccgaa aaggaatcag gcgtgacgtt
    20341 gaggatcccc attacgtgag gatggctaag gtccagtgaa gtaccctggg caaagagttt
    20401 catgatgtta tccctggtat gaattgataa gaaaaaaccc cggagcacgc cccggggttt
    20461 tcggtacaaa tacagtcatc tgatgcggga acttacttgt cgcctaactg ctctgacatg
    20521 gtgttacccg ggttcggcgt acgcggttca tcaaccggac gaggagcctt tggactacca
    20581 ttgtcgccag aattgttaga agcgcctggt tcttcccagc ccgctggcgg acgtacatcg
    20641 cgacgtgcca tcaggtcatc aatctgcggt gcgtcgatag tctcatattt catgagagca
    20701 tctttcatcg catgcagaat atccatattg tcggtcagaa gctgacgcgc acgattatag
    20761 ttacgctcaa tcagtgcttt cacttcctgg tcgatgatac gtgcagtttc atcggacata
    20821 tgtttcgctt tcgctacgct acggccgagg aatacttcac cttcttcttc cgcgtacagc
    20881 agtggaccca atttctcaga gaagccccac tgggtcacca tgttacgtgc cagattggtc
    20941 gcaactttaa tatcgttgga cgcaccggta gaaacatgtt ccggcccata gatgatctct
    21001 tctgccagac gaccaccgta cagcgtagaa atctggcttt ccagtttctg acggctggcg
    21061 ctgattgcgt cgccttcagg caagaagaaa gtcacaccca gcgcacgacc gcgtgggata
    21121 atcgtcactt tgtgcaccgg atcgtgttcc ggcaccaggc gaccgataat cgcatgaccc
    21181 gcttcgtggt aagccgttga ttctttctgc gcttccgtca tcaccatgga gcgacgttcc
    21241 gcacccatca tgattttgtc tttcgctttc tcgaactcaa ccatcgacac aacgcgtttg
    21301 ttgccacgag cagcgaacag tgccgcttcg ttcaccaggt tcgccaggtc agcaccggag
    21361 aaaccaggag taccacgggc aatgattgcc gcgtcgatat cgggggccaa tggtacgcga
    21421 cgcatgtgaa ctttcaggat ctgctcacga ccgcgaacat ctggcaagcc gaccacaacc
    21481 tgacggtcga aacggccagg acgcagcagg gccgggtcga gaacgtccgg acggttagtc
    21541 gcggcgataa cgatgatacc ttcgttacct tcgaagccat ccatctcaac cagcatctgg
    21601 ttcagagtct gttcacgttc atcgtgacca ccgcccagac cagcgccacg ctggcggcct
    21661 acggcgtcga tttcatcgat aaagatgatg cacggtgccg ctttcttcgc ctgttcgaac
    21721 atgtcacgaa cacgggatgc acccacaccg acgaacattt ctacgaagtc agaaccggag
    21781 atagtaaaga acggaacttt cgcttcgcct gcaatcgctt tcgccagcaa cgttttaccg
    21841 gtacccggag gaccgaccat caggacgcct ttcgggatct taccgccgag tttctggaag
    21901 cggctcggct cgcgcagata ctctaccagt tcagcaactt cttcttttgc ttcgtcgcag
    21961 cccgcaacgt cagcaaaggt cgttttgatc tgatcttccg tcagcatgcg cgctttgctc
    22021 ttaccaaacg acatggcacc tttgccaccg ccgccctgca tttgacgcat gaagaagatc
    22081 cagacaccaa tcagcaacag catcgggaac caggagatga agatagaagc cagcaggctt
    22141 ggttcttcag gcggttcacc gacaaccttg acgttcttgg tcaacaggtt atccagtaat
    22201 ttcggatcct gaaccggaat gtaagtggta taacggttac tatctttctt ggtaacgttg
    22261 atttcacgtc cgttgatacg cgcttcacga acctggtcgt tattgacctc ttgtaggaag
    22321 gtagagtaat ccaccttacg gccattagac tcgctgggcc caaagctctg gaatactgac
    22381 atcagcacaa cggcaatgac cagccagagt attaggtttt tcgccatgtc actcaaggga
    22441 ttaacctctt attacaactg tgttaaaaac agcgtcagga tactctatat ccagcatctt
    22501 tcaaactttc gtctgaaatc tcccggttag ggtttacgcc cggtcgctac aatatacact
    22561 tcccgcgaac gtgcacgaga agagtccggc ttacgaactt tgaccttcgt aaacagggag
    22621 cgaatttccc ttagatactc atcgaaacct tcgccctgga acaccttcac tacaaaactg
    22681 ccacctggcg ctaatacatc acgacacatt tctagcgcca gttccaccag atacatggca
    22741 cgggggatat ccaccgccgg tgttccgctc atgtttggtg ccatatcgga catgacaacc
    22801 tggactttgc tgtcgccaac gcgctccagc agtgctttca tcaccagttc atcacgaaaa
    22861 tcgccctgaa gaaagtccac accaacgata ggatccatag gtaaaagatc gcaagcgatg
    22921 atgcggcctt tgccgccaat ttgggtgacc acatattgtg accaaccacc cggagcagca
    22981 ccaaggtcga caaccgtcat tcccggttta aagagtttgt cactttgctg tatttcatca
    23041 agtttaaacc aggcacggga acgtaacccc tttttctgtg cctgttgaac atatttatcg
    23101 ctaaagtgtt cctgaagcca gcggctggag ctggcagaac gcttcttacc tgtcatttaa
    23161 ctttcccatg gggataactc atcgtaacca attgcgtaaa tttttacgcg cctatttggt
    23221 gatatatggg agatggcggt agaatgaccc gttttcaatc ccaacgtaag caaaaaatac
    23281 gatgaatctg agtactaaac aaaaacagca cctgaaaggt ctggcacatc cgctcaagcc
    23341 agttgttctg cttggcagta atggtttgac cgaaggggtg ctggccgaga ttgaacaagc
    23401 gttagagcac catgaactca tcaaggtgaa aatcgccacc gaagatcgcg aaactaaaac
    23461 cttgatcgtg gaagctatcg tgcgcgaaac cggcgcctgt aatgtacagg tcatcggtaa
    23521 aacgctggtg ctttatcgcc caactaaaga acgtaaaatc tcgctgccac gctaagatta
    23581 tcctaaagtt acacacattt gctgtgtaaa acgaggggtt ttccgcaggc aggagagcaa
    23641 aatgccacgc tctgttcgtt gataaaaggc cgcatagcgg cctttttcct ttctttacaa
    23701 tacatcaaca tcttgagtat tgggtaattc ttacaggtat tccaccttaa ttacttcaaa
    23761 ttctacttcg ccgcccggcg ttttgatgac cacaacatca tcttcttctt tgccgatcag
    23821 gccacgagca ataggcgagt ttacagaaat caggttttgt ttaaagtcag cttcgtcatc
    23881 gccaacgatg cgataagtct gttcttcgtc agaatccaga ttcagcaccg ttacggtagc
    23941 accgaaaata acgcgcccat tgttgggcat tttggtgaca tcaatcacct gcgcgttcga
    24001 cagcttggct tcgatgtctt taatacggcc ttcgcagaaa ccctgctgtt cacgagctgc
    24061 gtggtattcg gcgttttctt tcaggtcgcc atgctcacgc gcttccgcga tagcagcaat
    24121 gatttcagga cggcgcacag atttcagaaa atccagctct tcgcgtaatt tttcagcgcc
    24181 gcgtaaggtc atcggaatag cttgcatttg ttatacctct tgaatattcc tgatggggca
    24241 agtcttcacc caccccggct gttcggcctg cccggcataa ctcctcaccg ggaccagaag
    24301 caaaaaaata ccgacccggg tacaagtccc aggtcagcta caattcacat tttgatagtc
    24361 attttaccct gaagttcccg aagggtcatc gtttacttta tagggcgttg cgccgtagta
    24421 tgacggctcg attccaggtt gttagcgcga gattatgcga ttttccagat ttatcatcgg
    24481 attgaccagc tgtatagcgt tcagtgttca ggccgcgaat gttgatgagt acgttactca
    24541 actccccgct ggtgccaacc ttgccctgat ggtgcaaaaa gtcggcgcgt cggcccccgc
    24601 tattgattac cacagtcagc agatggcgct gcccgccagt acgcagaaag tgattactgc
    24661 gctggcggcg ttgattcaac tcggccccga ttttcgtttc accacgacgc ttgaaaccaa
    24721 aggcaatgtg gaaaacggcg tacttaaggg tgacttagtg gcgcgatttg gtgccgatcc
    24781 gacgttaaaa cgtcaggata ttcgcaatat ggtcgcgact ttgaaaaaat ctggcgtcaa
    24841 ccaaatcgat ggcaatgtgt tgatagatac ctccattttc gccagccacg ataaagcccc
    24901 cggctggcca tggaatgaca tgacacaatg ctttagcgct ccgcctgccg ctgccatagt
    24961 tgaccgcaac tgtttctccg tctcgctcta cagtgcccca aaacctggtg atatggcttt
    25021 tatacgcgtg gcatcttatt accccgttac gatgttcagc caggtacgca ccctcccccg
    25081 aggttctgcc gaagcgcaat actgcgaact ggatgtcgta ccaggcgacc tgaaccgctt
    25141 tacgctgacg ggatgcctgc cacaacgttc tgagccgctc ccgttggctt ttgccgtgca
    25201 ggatggagcc agctatgccg gggcaattct gaaagatgag ttaaaacagg caggtatcac
    25261 ctggagcgga acactgctgc gccagactca ggttaacgaa cctggaacgg tagttgccag
    25321 taaacagtcg gccccgttgc acgatctgct taagattatg ctgaaaaagt cggacaacat
    25381 gatcgccgat acggttttcc gcatgatagg ccatgcgcgc ttcaatgtgc ctggaacatg
    25441 gcgggccggg tcggacgccg tgcgtcagat cctgcgccag caagccggtg tcgatattgg
    25501 aaacaccatt attgccgatg gttcagggct ttcgcggcat aacctgattg cccccgccac
    25561 catgatgcag gtgctgcaat acattgccca acacgacaat gaacttaact ttatctccat
    25621 gctgccgctt gcgggctatg acggctcttt gcagtaccgt gcaggtctgc atcaggcggg
    25681 cgtggatgga aaagtctcag cgaaaaccgg ttcgttgcag ggggtatata acctggcggg
    25741 attcattacc acagcgagcg ggcaacgaat ggcgtttgta caatatcttt ctggctatgc
    25801 agtagaaccg gcggatcagc gtaatcgccg tattccgtta gtgcgttttg aaagccgttt
    25861 gtataaagat atttatcaga acaattagtc aaaaagaaac cccggcacat ggccggggtt
    25921 ttgctgatta acgcttgtaa atgaactcaa cgccttcttc gtcgtcttcg tcccagtcat
    25981 catcccagtc ctcatcatcc tcttcagcaa tctcttcaag ctgctggcga tgataatcat
    26041 cccacatgaa ttcgactttc tctggctgtt tcgcttcttc agcctgcacg accgggtttt
    26101 caatgataaa ggtcatcaca tcccagcaga gatctttcac gcccagtcca ctcgccgcag
    26161 agatcagata atatttatct tcccagccca gcgcttcagc aatcgctttc gctttctctt
    26221 cagcttctgc cttatccagc agatcgatct tattgaacac taaccaacgc ggtttcgccg
    26281 ccagatcctg gctgtatttt tccagctcgc tgataataat acgcgcgttt tcaaccggat
    26341 cggtgccgtc aatcggatcg atatcgatga ggtgcaacag gacgcggcaa cgttccaggt
    26401 gcttcaggaa gcggataccc agacctgcgc cttccgcagc gccttcaatc agtcctggaa
    26461 tatcggcaac aacgaagctc ttttcgttgt ccattcgtac cacacccaga cttggcacca
    26521 gagtggtaaa cggataatcc gccactttcg gtttagccgc cgataccgcg cgaataaagg
    26581 tcgatttacc cgcgtttggc atccccaaca taccgacgtc agccagcagc atcagctcca
    26641 gcagcagctc acgcttatca cccggcgtac cgttggtttt ctgccgtggt gtacggttaa
    26701 cggacgattt gaaacgggta ttgcccagac cgtgccagcc gcccttagca accagcagac
    26761 gctgaccgtg tttagtcata tcgcccatgg tttcaccagt accttggtcg attatacgcg
    26821 tacctaccgg cactttaatc gtcacgtctt taccgcgctt accggtacaa tcgcggcttg
    26881 cgccgttctg accgcgctct gcacggaaag atttttcaaa acgataatcg ataagcgtgt
    26941 tcaggttttc gtcggcttcc atccatacgt caccaccatc accaccgtcg ccgccatccg
    27001 ggccgccttt cggaatatac ttttcgcggc ggaagctcac gcaaccatta ccgccatcac
    27061 ctgcaacgac cagaatcgat gcttcatcaa caaacttcat tttattctcc gtaaatcatt
    27121 cgcctgcgcg gggttgcgat accaccgttg tatgcttacg taatccgccc caaatacgat
    27181 gaccaatggc ggaatacatc gcgcccgcaa ccacgacaaa cgcaccgaga taacctaaaa
    27241 ggtttaacat cggtctggcg aagaaatcgg gccaggccag tgataaaaga tctgaaaaaa
    27301 acagcgtaaa cagtggggtg agcgtgatga tcgcgctcac ctgcgctgcc tgccagcgag
    27361 ccatcgcttc cgccagggcg ccatatccta ccaatgtatt cagtccgcaa aaaattaaac
    27421 atgcgagctg ccagtggcta agctgcgcta tcactccagg ctttgccaga gggaagagcg
    27481 caattgtaca taaagcgtac agtaaaaaca ggatctgcgg tgaggccagc cgacgcaata
    27541 aaaccttttg cgccacgcca taactcaccc aaaccgtcgc cgcaccgacc ccaaagataa
    27601 ctccccaggt gtaatcggtg agctttgtaa atatctcgac cagactggtg ttaaaaaaca
    27661 tcaccaggcc gctcaggagc atcaatgccc ctacaacctg agtgctgcgc attttctctt
    27721 tcaggataaa tacgctggca accatcatgc caactggcga gagttgccca atcacctgcg
    27781 aagcggtcgg actcaggtat tgcaaggatg agctgaacag gatgaagttc ccaaacagcc
    27841 cggcggtcgc cactgccaac aaaatcaacc agcgtggctt acgaaacacg cgtaatggcg
    27901 gcaacctctt cttcaccgca agaatggcac caaggccaat actcgccatc aagaaacggt
    27961 aaaacacgat tgtcggaggt tccatcacct ccagcacctg cttcattgcg attggcaacg
    28021 ccccccagca aattgctgtg gtgagcgcca aaagaatgcc aatgcctgcc tgctgcttca
    28081 taccgtattc cctgcaaagc gcatttgcgg attaacgtcg caccatgcgc atttttaccg
    28141 gttatcgaat gtaaaaagcc ccgcaacgtg ttgcggggct ttcatccgtt accgggacgc
    28201 gaaaaactta ttcagcttcg atgctgataa atttacggtt tttcgggcct ttaacttcga
    28261 atttcacttt accgtctgct ttagcaaaca gagtgtggtc acgaccgcaa cctacgttag
    28321 cgccagcgtg gaatttggta ccacgttgac gaacgatgat gctacccgcc agaacggatt
    28381 cgccaccgaa acgcttaacg cccaagcgtt tagcttctga atcgcgaccg ttacgtgtgg
    28441 agccgccagc ctttttatgt gccatttgaa atctctcctc aggtcttagg cgctgatgcc
    28501 agtaattttc acatcagtga accactgacg atggccctgc tgcttacgat agtgtttacg
    28561 acgacgaaac ttaacgattt taactttctc gccacgaccg tgagcaacaa cttcagcttt
    28621 gattacgccg ccatcaacga aaggaacgcc gattttgact tcttcaccgt ttgcgatcat
    28681 cagcacttca gcgaactcaa cagtttcgcc agttgcgatg tccagctttt ccaggcgaac
    28741 ggtctgacct tcgcttactc ggtgttgttt accaccactt tggaaaaccg cgtacatata
    28801 aaactccgct tccgcgcaca ccttttcaat gattcagagt gcgctataaa tattcacaat
    28861 agggcgcgaa tattacgcaa aacgcacgcc tttgacaagt gctacagtca atacacgaag
    28921 aaaaaaaaca caacttgtac ggtaacgttt atctgtgcca ttttttcagt acaatcactc
    28981 tatattccta accataaacc ctaagttgcc tttgttcaca gtaaggtaat cggggcgaaa
    29041 agcccggctt ttgcgatgaa tttagaaaaa atcaatgagt taaccgcgca agatatggcg
    29101 ggtgttaatg cggcaatcct tgagcagctt aattccgacg tccagctgat caatcagtta
    29161 ggctattaca tcgtcagcgg cggcggtaaa cgtattcgtc cgatgattgc tgtactggct
    29221 gcacgagctg ttggctatga gggaaatgcg catgtcacca ttgctgccct gatcgagttt
    29281 atccacacgg cgactctgct gcacgacgac gttgtggatg aatcagatat gcgcaggggt
    29341 aaagctaccg ccaacgctgc atttggcaat gccgccagcg tgctggtagg cgattttatt
    29401 tatacccgcg cgttccagat gatgaccagc ctcggttcgc tcaaagtgct ggaagtcatg
    29461 tcagaagccg taaacgtcat cgcagaaggt gaagttctgc aactgatgaa cgttaacgat
    29521 ccggacatca ctgaagaaaa ctacatgcgc gttatctata gcaaaaccgc gcgtctgttt
    29581 gaggctgccg cgcagtgttc cgggattctg gctggctgta cgccggaaga ggagaaaggc
    29641 ctgcaggatt atgggcgcta tctcggcact gctttccagt tgatcgacga tttactcgat
    29701 tacaatgccg atggcgaaca gttaggtaaa aatgtcggcg acgatctgaa cgaaggtaaa
    29761 ccgacgctgc cgctgctgca tgcgatgcat catggcacac cagaacaggc acagatgatc
    29821 cgtaccgcca tcgaacaggg taacggtcgc catcttctgg aaccggttct ggaagcaatg
    29881 aacgcttgtg gatctcttga atggacgcgt cagcgtgccg aggaagaagc agacaaagcc
    29941 atcgcagcgt tacaggtgct cccggacacc ccttggcgag aagcactcat cggcctcgcg
    30001 cacatcgctg ttcaacgcga tcgttaatcc cctcccctca tcccgcgcag cgcgcggggt
    30061 ggttccagta aattccataa aaacacttac tcagctctat aacacctgaa aaacaggctg
    30121 tgcatcttta tatattctga atattcacac tccttacagg aactttttag agcaatcggc
    30181 catcaggagt atagtgatgc tcgacagaag aagtgttctg aatgaaagcg actaacttaa
    30241 ggagtgagga aagatggaaa gtaatttcat tgactggcat cccgctgaca tcattgcggg
    30301 tttgcgcaag aagggaacgt caatggcggc ggagtctcgc agaaatggtt tgagttcctc
    30361 aacgctggcg aatgcattat cgcgcccatg gccgaaagga gagatgatta ttgcgaaagc
    30421 cctgggaact gacccctggg ttatctggcc atcacgctac catgatccgc agacccatga
    30481 gtttatcgac agaacgcagt tgatgcgtag ctacactaaa ccgaaaaaat gagtggtctg
    30541 gcggtagccc cgcgaacggg gctgccagct ctcagacgat tactcgcctt tcacacgctc
    30601 aatatttgca cctaaagcgc gcagtttgtc ttcaatgcgt tcgtagccac gatcgatgtg
    30661 ataaatacga tcaaccaccg tcgtcccttc cgcaatacag ccagccagca ccaggcttgc
    30721 tgatgcacgc agatcggttg ccataacctg tgcgccagaa agtttttcaa caccgtgaca
    30781 aataacggta ttgctttcga tttcggcatg cgcgcccata cggctaagct ccggcacatg
    30841 cataaagcgg ttttcaaaga ccgtttcggt gatgaatccg gtcccttctg ccaccaggtt
    30901 caacagcgtg aactgggcct gcatatcggt cgggaatgcc ggatgcggcg cggtacgtac
    30961 gttaacagcc ttcggacgtt tgccatgcat atccaggcta atccagtctt cgccgacttc
    31021 gatgtccgct ccagcgtcac gcagtttcgc cagcacggcg tcgagagtat ctggctgcgc
    31081 gttacggcag ataattttgc cgcgagaaat cgccgccgcc accaggaaag taccggtttc
    31141 gatacgatcc ggtagcacgc gatagacacc gccgcctaaa cgttccacac cttcgatgac
    31201 gatacgatcg gtgccctgac cgctaatttt cgcacccagc gtaatcagga agttcgcggt
    31261 atcgacgatt tccggttcac gcgctgcgtt ttcaataatc gtggtacctt ccgccagggt
    31321 tgcagcacac atgatggtca ccgttgcgcc aacgctgact ttatccatca cgatatgtgc
    31381 gcctttcaga cgaccatcga cggaagcttt aacgtaacct tcttccagtt tgatggtcgc
    31441 gcctaattgt tcgaggccag aaatgtgtag atcaaccgga cgcgcaccga tcgtacaacc
    31501 gccaggcagt gaaacttgcc cctgaccaaa gcgcgctacc agcggcccca gtgcccagat
    31561 agaagcacgc atggttttaa ccagatcgta aggtgcgcag aatacattaa cgtcgcgggc
    31621 atcaatatgc acagaaccat tacgttctac tttcgcaccc agctggctta gcagcttcat
    31681 tgatgtatcg acgtctttca gtttcgggac gttctggatc tctaccggtt cttccgccag
    31741 tagagcggca aaaaggatag gcagagcagc atttttagcg ccggaaattg tgacttcgcc
    31801 ctggagcttc gttggcccct gaacacgaaa tttatccatt tagtttgttc tcagttaaca
    31861 attcatatcc gctaccggcg aatcgcccat agctcaaaag ccgttcagtt tgcgatcgcg
    31921 cgcccactcc gcaggggtat acgctttgat cgacacagca tgaatgcggt tatccgcaat
    31981 atattccatc agcgggccat agaccgtctg ctgtttttta acccgactca tgccgtcaaa
    32041 caactcaccc acggcaataa cctgaaagtg gctgccatcg ccggaaacgt ggacttcctg
    32101 gagggagaga gcgttcatca acacgctctg aatttcatta ttttccatgg gatcttcaat
    32161 catcagttaa taaaccagcg aaacatctta gagcaaagtt gcgctggcat aaataagcaa
    32221 aaagcctcgc tgataaatca gacaaggctc gacttgcagg caggtttgcc ggacaggcgg
    32281 ttaacgccat atccggcctg aaaaaattta acgaggcaga acatcagcag gcaaattata
    32341 caatttcgcg agggtataca ctttgtcgtt taccccctga agcgtcacgt tgttgccctg
    32401 ctttttcgcc agatcgataa gatggagcag cagtgccagt ccacccgtat ccacgcggga
    32461 cacacggcta agatcgatgc aggtaatccc cttcaccgct tcctcacgca tttcccaaag
    32521 cggtagcaga acgtcctgat ccagctctcc ggataacgcc agcgtgtcac ccgtctgcat
    32581 ccagctcagt gactcgctca ttattttttc tcttccagag tgattttctg ttgagaaatc
    32641 gatttcagtt gcgcagtcag gccgtcgata cctttggtac gcagcagcgt tccccactcg
    32701 ttttgtttgg tggtgatcat actgacgcct tcagcaatca tgtcgtaagc ctgccagttt
    32761 cccgtctggg agtttttacg ccactggaag tccagacgca ccggcggacg gccattcggg
    32821 tcaataatgg taacgcgaat aggcacaatg gttttatcgc ccagcggctg ttctggcgca
    32881 atctgatagg tttgaccgtg atacatcgcc agcgcctgac cgtaagcctg cttcaggtac
    32941 tcacggaaag cggcaaagta ggcttcacgt tgagcagggg tcgcactctt gtaatactgg
    33001 cccagcacca gcgcaccggc gtatttcacc tgtacgtatg gcagcagttc ctgatcaaca
    33061 atggtacgca gataatccgg gttggcccga atttgcggtt gctcattctt caggcgatcg
    33121 aacgttttct gcgccgcctc gtccatcagc ttatacggat tggtctggtc tgccgcggtt
    33181 gccgcactca gaggtgcaat caccagcaaa gcgaccatca ttaaacgttt aaacatgcgt
    33241 cggttctcct gaaattattt cgttgtaccc acaggttcag tggtttcatt attacctggc
    33301 gcagcagctg gcgcatcgcc actattctta ttgtcatcgc ctttactacc gtaaaggaac
    33361 tgaccaatga gatcttccag caccatcgca gacttagtgt cctgaattgt atcgccatcc
    33421 ttcaggatag cagtccccag ttccgggtct tcaaaaccga cgtttaatgc cagatattgt
    33481 tcccccagca ggccggaagt acgaatgctc agcgaactgg tatctggaat gtggttataa
    33541 cgttgttcaa tttccagcgt tacgcgtggc agataggttt tcgggtccag cgtaatatcc
    33601 gccacccggc ccacaacaac gccaccaata ctgaccggag agcgcgcttt caggccgcca
    33661 atgttatcga acgtcgcata aagcgtgtag gtcggttcag tacgtatgga cgtcacgttc
    33721 gccgccttca ggcaaacaaa cagcgccgcc agcagtgctg ctaataaaaa gatacccacc
    33781 caaatttcat tttttttcgt ttgcatgaac tcaattccca aacatcaatg cggtcagcac
    33841 aaaatccagc cccagaacag ccagagacga gtggacaacg gtgcgagtgg ttgcccggct
    33901 aatcccggca gacgtcggga tggcgtcgta gccgttaaac aacgaaatcc acgtcaccgt
    33961 gatggcgaac accacgctct taatcagaca gttgaccaga tccatacgcc agtcgacggc
    34021 attttgcatt gccgaccaga agaacccgct atcaatgcct ttccaactga cgccgaccag
    34081 agatccgccc cagatcccca cggcgacgaa aataaccgtc aacagtggta atgaaataac
    34141 cccagcccag aaacggggag aaataacccg acgcagcgga tccaccgcca tcatctccat
    34201 gctggagagt tgctctgtag cgcgcatcag gccgatttct gcggttagcg ccgaaccagc
    34261 acgcccggca aacaacaacg cggcaaccac cggccccagt tcacgcagta gcgataacgc
    34321 caccagcata cccagactgg tttccgcact ataagtggtc agaaccagat aaccttgcag
    34381 ccccaacacc attccgatga acacgccaga aaccacaata atcagcatcg acaggacgcc
    34441 gacattatag agctggcgca ccagcagcgg cgcatgtttg cgaaattccg gtttgccgac
    34501 cagcgcattg aataacatta acccggcccg cccgaacgtt ctcagggttt taatcccttt
    34561 atgtccgagc gacgccagcg catttaacag catgagtggc ttaactccct ggtaaaagat
    34621 cagcgtgata atcgccggca ggatagcgga acggaacagg cccgtcagct atcccgtcca
    34681 gaaactgacg tacgcgcgga tcaggattcg cctgcaacgc ctgggcactg ccatgagcga
    34741 caattttttt gtccgccagg atccaggcgt gatccgcaat acttaacact tccggcacat
    34801 cgtgagaaac caccacacaa gtcacgccca gcgcgctgtt cagctcagaa atcagcttca
    34861 ccagtacgcc catggtgatg ggatcttgcc caacaaaagg ttcatcaaac atgatgagat
    34921 ccggctccag cgcaatcgca cgcgccagcg ctgcacgccg cgccatccca ccagaaagtt
    34981 cagaaggcat tagcttagcc gctccacgca gccccacggc ctccagcttc atcatcactg
    35041 tactatgcaa caatggcgcg ggaagttggg tatgttcgcg cagtggatag gcgacgttgt
    35101 caaatacgtt catatcagtg aacaacgccc cggactgaaa taacatgctc atccgtttgc
    35161 gcactgtata caggcgcgaa cgagacatcg ccggaatatt ctcaccatcg aaaaggatct
    35221 caccatgatc tggtgcgatt tgcccgccaa tcagacggag tagcgtcgtt ttaccgatgc
    35281 ccgatggccc catgattgcc gtgatcttcc ctcgcggcac ggtcagggaa atattatcga
    35341 agatgcagcg attgccacgc gtaaaactga catcgcgcat atcgactaaa ttcgccacag
    35401 actgctccat aattcaccct tcgtcttgcg ttgattttct aagcatggcg ctcaatttaa
    35461 ccttgaaccc aacatattta cagaatatta cccgccgtgg ttagcgaaag ctggcatttg
    35521 ttttactttt tagccgcata aagtcaaaat taagaatccg ttacggcttt ctgaaaatct
    35581 tcagcggacc ggcgagtata cctgaagaaa ggatgttaga tgcttttagc tacggcactg
    35641 ttaattgttg gtttactttt ggtcgtttac agtgccgacc gcctggtttt tgccgcgtct
    35701 attctttgcc gaacctttgg catcccgccg ctgatcatcg gcatgacggt ggtcagtatt
    35761 ggtacatcgt taccagaagt catcgtctcg cttgccgcgt ctctgcacga acaacgcgat
    35821 ttagccgttg gtacagccct cggctcaaac attatcaata tattgctgat cctcggtctg
    35881 gctgcgctgg ttcgtccttt taccgtccat tctgatgttc tacgccgtga attaccctta
    35941 atgttgttgg tcagcgtggt ggccggttcc gtactctatg acggacaact tagtcgcagc
    36001 gatggtatct ttctcctctt tctggctgtg ctatggctgc tgttcattgt taaacttgca
    36061 cgtcaggctg aacgtcaggg gactgacagc ctgaccagag agcagcttgc agagctgccg
    36121 cgtgacggcg gattgcccgt cgcgttttta tggctcggca ttgcgcttat catcatgcca
    36181 gtggccacgc ggatggtggt tgataacgcc acggtgctgg cgaattactt tgccatcagc
    36241 gagttgacga tgggtctgac ggcaattgct atcggaacca gcctgccgga actggcaacc
    36301 gcaatagcgg gggttcgcaa aggtgaaaac gacattgctg tcggaaatat cattggcgca
    36361 aacattttta atattgtcat cgtgttgggt ttacccgcgc tgataacgcc aggagagatt
    36421 gatccactgg cgtacagtcg tgactacagc gtgatgttgc tggtgagcat tatttttgcg
    36481 ttgctgtgct ggcggcgctc cccacaaccg ggccgtggtg taggggtatt attaactggc
    36541 ggatttatcg tatggctggc gatgttgtac tggttatcgc caatactcgt tgaataactg
    36601 gaaacgcatt atgtcgcacg tagagttaca accgggtttt gactttcagc aagcaggtaa
    36661 agaagtcctg gcgattgaac gtgaatgcct ggcggagctt gatcaataca tcaatcagaa
    36721 tttcacgctt gcctgtgaaa agatgttctg gtgtaaaggg aaagttgtcg tcatggggat
    36781 ggggaaatcg gggcacatcg ggcgcaaaat ggccgcaacg tttgccagca ccggtacacc
    36841 ttcatttttc gtccatcctg gtgaagccgc gcatggtgat ttaggcatgg tcaccccaca
    36901 ggatgtggtg attgctatct ctaactcagg tgaatccagc gaaatcacgg ccttaattcc
    36961 tgtgcttaag cgtcttcacg taccgttaat ctgcatcacc ggtcgcccgg agagcagcat
    37021 ggcgcgcgct gcagatgtgc atctgtgtgt taaagtagcg aaagaagcct gtccgttagg
    37081 gctggcaccg accagcagca ccaccgccac gctggttatg ggcgatgccc tcgctgtcgc
    37141 gctgttaaaa gcacgcggct ttactgctga agattttgcg ctctcacacc caggcggcgc
    37201 actgggtcgt aaacttctgc tgcgcgtaaa cgatattatg catacgggcg atgagatccc
    37261 gcatgttaag aaaacggcca gtctgcgtga cgcattgctg gaagttaccc gcaaaaatct
    37321 tggtatgact gtcatttgcg atgacaatat gatgattgaa ggcatcttta ccgacggtga
    37381 tttacgccgt gtcttcgata tgggcgtgga tgttcgtcag ttaagtattg ccgatgtgat
    37441 gacgccgggg ggaatacgtg tgcgccctgg cattctggcc gttgaggcac tgaacttaat
    37501 gcagtcccgc catatcacct ccgtgatggt tgccgatggc gaccatttac tcggtgtgtt
    37561 acatatgcat gatttactgc gtgcaggcgt agtgtaaaga ttcaaggata aacaacaatg
    37621 agcaaagcag gtgcgtcgct tgcgacctgt tacggccctg tcagcgccga cgttatggca
    37681 aaagcagaga acattcgtct gctgatcctc gacgttgatg gcgtactgtc agatggcctg
    37741 atttatatgg gcaataacgg cgaagagctg aaagcgttca atgttcgtga cggttatggc
    37801 attcgttgtg tgctcacctc tgatattgaa gtcgctatca ttaccgggcg aaaggctaaa
    37861 ctggtagaag atcgttgtgc cacattgggg atcactcact tgtatcaggg gcagtcaaac
    37921 aaactgatcg cctttagcga tttgctggaa aaactggcga ttgccccgga aaatgtggct
    37981 tatgtcggcg atgatctcat cgactggccg gtaatggaaa aagtgggttt aagcgtcgcc
    38041 gtggccgatg cgcatccact gttgatcccg cgcgccgatt acgtgacgcg cattgctggc
    38101 ggtcgtggcg cagtgcgcga agtttgcgac ttattactcc tggcgcaggg caaactggat
    38161 gaagccaaag ggcaatcgat atgagtaaag ccagacgttg ggttatcatt gtgctatcac
    38221 tggcggttct ggtgatgatc ggcattaata tggccgaaaa agacgatacc gcccaggtgg
    38281 tcgtcaacaa caatgatccc acctataaaa gcgagcatac ggacacgctc gtctataacc
    38341 cagaaggggc actaagctat cgattgattg ctcaacacgt tgaatattat tccgatcagg
    38401 ccgtttcgtg gtttacgcag ccggtactta ccacgtttga taaggataaa atcccgacat
    38461 ggtccgtaaa agcagataaa gccaagctga ccaatgaccg gatgctctat ttatatggac
    38521 acgttgaagt caacgcactc gtgccggact ctcaacttcg cagaatcacg acggataacg
    38581 cgcagatcaa tctggtgacg caggatgtta cctctgaaga cctcgtcacg ttatacggaa
    38641 caacatttaa ctccagcggt ctgaaaatgc gcggcaactt acgcagcaag aacgccgagc
    38701 tgattgaaaa ggttagaaca tcctatgaaa ttcaaaacaa acaaactcag ccttaatctt
    38761 gtgcttgcca gctcacttct ggccgccagc attccggcat ttgccgtaac cggagacact
    38821 gatcagccga tccacattga atcggaccag caatctcttg atatgcaagg caacgtggtt
    38881 acctttaccg gtaatgtcat cgtcacccag ggcaccatca aaattaatgc cgacaaagtg
    38941 gttgttaccc gtccgggcgg cgaacaaggt aaagaagtga ttgacggcta cggtaaaccg
    39001 gcaacgttct accagatgca ggacaacggt aaaccggttg aaggtcacgc ttcccagatg
    39061 cactacgaac tggcaaaaga ttttgtcgtt ctgacgggta atgcttatct gcagcaggtt
    39121 gatagcaaca ttaagggcga taagatcact tacctggtaa aagagcagaa aatgcaggct
    39181 ttcagcgaca aaggcaagcg cgtaaccacc gttctggtgc cgtcgcagct gcaggacaaa
    39241 aacaacaaag gccagacccc ggcacagaag aagggtaatt aattcgttat ggcaacatta
    39301 actgcaaaga accttgcaaa agcctataaa ggccgtcgcg tggtagaaga cgtcagcctg
    39361 accgtcaact ccggggaaat tgtcggtctg ctggggccaa acggtgccgg taagaccacc
    39421 actttctata tggttgtagg cattgtgccg cgcgatgcgg gcaacatcat tattgatgat
    39481 gacgatatca gtctgctgcc tctgcatgca cgcgcgcgcc gcggtatcgg ctatctgcca
    39541 caggaagcct ccattttccg tcgcctcagc gtttacgata acctgatggc ggtactgcaa
    39601 attcgtgacg acttgtctgc tgaacaacgt gaagaccgcg cgaacgagct gatggaagag
    39661 tttcacattg agcacctgcg tgacagcatg gggcagtcac tctccggggg tgaacgtcgc
    39721 cgtgtagaaa ttgcccgcgc actggctgcg aatccgaaat ttattctgct cgacgaaccg
    39781 tttgccggtg ttgacccgat ctcggttatc gacattaaac gcatcattga gcacctgcgc
    39841 gacagcggcc tgggcgtgct gatcactgac cacaacgtgc gtgaaacact ggcggtttgt
    39901 gaacgcgctt atatcgtcag tcaggggcat ttgatcgccc acggcacgcc tacagaaatc
    39961 ttacaagacg aacacgttaa gcgtgtatac cttggggaag acttcagact ctgatagggt
    40021 agaagtttgc gacgttttag caggagagta cgattctgaa catgaagcaa ggtttgcaac
    40081 tcaggcttag ccaacaactg gcgatgacgc cacagctcca acaggcaatt cgtctgttgc
    40141 agttgtcgac gctggaactt cagcaggagc tacagcaggc gctggagagt aatccgctgc
    40201 ttgagcaaat cgacactcat gaagaaatcg acacccgcga aacgcaagac agtgaaacgc
    40261 tggacaccgc cgatgcgctc gaacaaaaag agatgccgga agagctgccg ctcgatgcca
    40321 gttgggacac catttacacc gctggtacac catccggcac cagcggtgac tacattgacg
    40381 atgagctgcc ggtctatcag ggcgaaacga cgcagacctt gcaggattac ctgatgtggc
    40441 aggtcgagct gacaccgttt tccgacactg accgcgctat tgctacctct atcgtcgatg
    40501 ccgttgatga caccggttat ctgactgtcc cgctggaaga tattctcgaa agtatgggcg
    40561 atgaagagat cgacatcgac gaggttgaag ccgtccttaa gcggatccaa cggtttgatc
    40621 cggtcggtgt agcggcaaaa gatctgcgtg actgcctgct aatccaactc tcccaattcg
    40681 ataaaaccac gccgtggctg gaagaggcca gattgatcat tagcgatcat ctcgatctgt
    40741 tagccaatca cgacttccgc actttaatgc gcgtcacgcg tctgaaagaa gatgtgctga
    40801 aagaagccgt caatctgatc cagtcgctcg atccgcgccc cgggcagtcg atccagactg
    40861 gcgaacctga gtatgtcatt ccagatgtgc tggtgcgtaa gcataacggt cactggacag
    40921 tagaactcaa cagtgacagc attccgcgtc tgcaaatcaa ccagcactac gcctcgatgt
    40981 gcaataacgc tcgcaatgat ggtgacagcc agtttatccg cagcaatctg caggatgcca
    41041 aatggttgat caagagtctg gaaagccgta acgatacgct actgcgcgtg agtcgctgta
    41101 tcgttgaaca gcagcaagcc ttctttgagc aaggcgaaga atatatgaaa ccgatggtac
    41161 tggccgatat cgcccaggct gtcgaaatgc atgaatcgac gatatctcgc gtgaccacgc
    41221 aaaaatacct gcatagtcca cgaggcattt ttgaactgaa gtatttcttt tccagtcacg
    41281 tcaataccga gggcggcggc gaagcttcct ccacggcgat tcgtgcgctg gtgaagaaat
    41341 taatcgcggc ggaaaaccca gcgaaaccgt tgagcgacag caagttaacc tctttgctgt
    41401 cggaacaagg tatcatggtg gcacgccgca ctgttgcgaa gtaccgagag tctttatcca
    41461 ttccgccgtc aaaccagcgt aaacagctcg tttgacccaa ccgataagga agacactatg
    41521 cagctcaaca ttaccggaaa taacgtcgag atcaccgagg cactgcgcga atttgttaca
    41581 gccaaatttg ccaaacttga gcaatatttt gaccgaatca accaggtcta tgttgttctg
    41641 aaagtggaga aagtcaccca cacctcagat gcaacactgc atgtaaacgg tggtgaaatt
    41701 catgccagcg cggaaggtca ggacatgtac gctgccattg atggcttaat tgataagctg
    41761 gcacgccagt tgaccaaaca taaagataaa ctgaaacaac actaattgtc cgggcaatta
    41821 gcatgtgcat ggcggcctgt tgtgcggcac aacgggccat ttgtacggtt aatgctccga
    41881 gcctgttcca ctgtttgagt gggcaggttc ttaggtgaaa ttatgacaaa taatgataca
    41941 actctacagc ttagcagtgt tcttaacagg gaatgtacgc gaagccgcgt ccactgtcag
    42001 agcaaaaaac gcgccctgga aatcatcagc gagctggcgg cgaaacaact tagcctgccg
    42061 cctcaggtgg tttttgaagc tatcctgacg cgggaaaaaa tgggcagtac cggtatcggc
    42121 aatggtattg ccattccgca tggcaaactg gaagaagata ctctgcgcgc cgttggcgtt
    42181 ttcgttcagc tcgaaacgcc tatagctttc gatgccatcg acaaccaacc ggtggactta
    42241 ctttttgccc tgctggtgcc ggcagaccaa actaaaacgc acctgcatac tctgtcgctg
    42301 gtggcgaaac gtctggcgga caaaaccatc tgccgccgtt tacgcgcagc ccagagcgat
    42361 gaagagctgt atcaaatcat tacggatacc gaaggtactc cggatgaagc gtagttattc
    42421 ggtaatgtct cttttagacg ttgtgaggag aaacagtaca tggtactgat gatcgtcagc
    42481 ggacgttcag gttcaggtaa atctgtcgcc ctgcgtgcgc tggaagatat gggtttttac
    42541 tgcgtggata accttcccgt agtgttgtta cccgatctgg ctcgaactct ggccgatcgc
    42601 gagatttctg ccgccgtcag cattgatgtt cgtaatatgc cggagtcacc agaaatattc
    42661 gaacaggcga tgagtaacct gcctgacgct ttctcaccgc aactactgtt cctggatgcc
    42721 gaccgtaata ccttaattcg tcgttacagt gacacgcgcc gactgcatcc gctttccagc
    42781 aaaaacctgt cgctggaaag tgctatcgac aaagaaagcg atttgctgga gcctctgcgt
    42841 tcgcgagcgg atctgattgt cgatacctca gaaatgtccg ttcacgagct ggctgaaatg
    42901 ctgcgtaccc gtctgctggg taaacgtgaa cgcgaactga ccatggtctt tgagtctttc
    42961 ggcttcaaac acggtatccc tatcgatgca gattacgtct ttgacgtgcg cttcttgccg
    43021 aacccgcact gggatccgaa actgcgtcca atgacaggtc ttgataaacc tgtcgccgcg
    43081 ttcctcgacc gccacacaga agtacacaat tttatctacc agacgcgaag ctatcttgaa
    43141 ctatggttac ctatgctgga aaccaacaac cgtagctact tgacggtcgc cattggttgt
    43201 accggcggaa agcaccgttc ggtgtatatt gcagagcaac tggcagacta cttccgctcg
    43261 cgcggtaaaa acgtccagtc acgccatcgt acgctggaaa aacgtaaacc atgaccgtca
    43321 agcaaactgt tgaaatcaca aacaagctgg gcatgcatgc ccggcctgca atgaagctgt
    43381 ttgaattaat gcagggtttt gacgctgaag tgctgttacg taatgacgaa ggcaccgagg
    43441 ccgaagccaa cagcgttatt gcgctgctga tgttggattc tgccaaagga cggcagattg
    43501 aagttgaagc gaccggccca caggaagagg aagcactggc cgccgttatc gccctcttta
    43561 actctggttt tgatgaagat taatcttcat cactttgaca tacaactccc ttcaaactcc
    43621 ccccgacaat aagaaaatca cgtactgaaa tcgttctcaa tcaacgtcat ttgtacattt
    43681 tgtgcgcttt tcactttcag aagaacctta agaaaacctt aagagacatt gtttaggttt
    43741 tgtttaagtt aatcgaccat actggagatc gtcagaaaat atttccagga gatggcatga
    43801 ttcgcttatc tgaacaaagt cccctgggca ccggaagaca tcggaaatgt tatgcgcatc
    43861 cggaagatgc ccaacgctgt atcaagattg tctaccatcg tggcgatggt ggcgataaag
    43921 agatccgccg tgagttaaag tactacgcgc atcttggtcg ccggttaaaa gactggagtg
    43981 gaataccgcg ctatcacggt accgttgaga cggattgcgg aaccggttat gtctacgatg
    44041 tgattgcaga ttttgacggc aaaccctcca ttacgctgac cgaatttgct gaacaatgcc
    44101 gttacgaaga agacattgca caactgcgcc agctactgaa acagctgaag cgttatttgc
    44161 aggataaccg tatcgtgacg atgtcgctga agccgcagaa tatcctctgt caccgcatca
    44221 gtgagtcaga ggttatcccg gtcgtctgtg acaacattgg tgaaagcaca ttgattccac
    44281 tggcgacctg gtcgaagtgg tgttgtctgc gtaagcaaga gcggttatgg aaacgattta
    44341 tcgcccaacc ggcgctggct attgccctgc agaaagatct gcaacctcgt gagagcaaaa
    44401 cgctggccct cacttcgcgc gaagcttaat ccagctggtg ttgttgcata aacggctcac
    44461 cgcctaactg atacatctgc cgtaaaatcc acgcctgacg gctacgcacg tagcccgatg
    44521 gcgaggagac tttgaaacga agtggattag gtaatacagc tgccagtaat gcagcttccg
    44581 accgggtaag tttgctcgcg ggtttgtgga aataacgttg tgccgcagct tcgacgccaa
    44641 acacaccgtc gccaaattcg gcgatattca ggtaaaccgt caggatacgc tttttgctcc
    44701 agactgtttc tatccccagc gttaatccag cttccagccc ttttcgtacc cagctacgcc
    44761 catcccataa aaagagattt ttggccgtct gctgagaaat cgttgaagca ccgcgaatac
    44821 ggttttcatt gcgctcgttg tgcgccaggg ctttctcaat ggaagcgaca tcaaagcccc
    44881 agtgctcagg aaatttctga tcttctgcgg caatcaccgc cagtcccatc cacggcgaga
    44941 tttgatccat actgacccag tcagaatgtg ccacgtaacg aaaattgcca tgcagccagg
    45001 cgctgacctg tcgctcgacc attaccgctg agaagggaac aggcgcaaca ctaaacaacg
    45061 cgatgccccc gccccagaaa acggcgagga caaccattaa ccgcaaaaga aaacggcgaa
    45121 cgaaactaaa caccgttaag cggcttttac tcattcagcc agaaccagca cgcgggaaac
    45181 cagcttatca atgccgctcg ccgcttctgc aatgttctgc gccagcatat atgctggggt
    45241 ggtgacaatc ttattgtctt catcaaccac gatatcatcg acaggacagg gcacatgctc
    45301 cgcgcccatc tcttccagca cttctgcggt atcgatatca gtaccgatgg tcaaacgcag
    45361 cgggaaatcg aaaattttcg gcagcatcgc cggggcaata cacataaaac caagcggttt
    45421 tccggcctga tgcatcgctt gtgccagcgc ctttaattca cggtcaacgg tgcattcgct
    45481 accaagagtg gcaaaattgc ttaaattctt cgccgcgcca aacccccccg gcacaatcaa
    45541 cgcatccagt tcagcggcat cggcctgggc cagaggacgg atttcaccgc gcgttattcg
    45601 tgccgcttca atcagcacgt tgcgcgtttc cgtcatcgct tcgccagtta aatggttgat
    45661 aacatcaacc tgctgcttat ccggtgcaaa gcagaccgcc tgcgcaccgc tgcgtgaaat
    45721 agctaacaac gtcaacaccg cttcatgaat ttcagaaccg tcatagacgc cgcatccgct
    45781 cagaattacg ccaattttct tcattgtgat catcctttcg caattgactg aaacacatta
    45841 atttttttaa taaaaatggt acgcatcaca catttaactg attcatgtaa caaatcattt
    45901 aagttttgct atcttaactg cgtgcggcct gaaaaacagt gctgtgccct tgtaactcat
    45961 cataataatt tacggcgcag ccaagatttc cctggtgttg gcgcagtatt cgcgcacccc
    46021 ggtctagccg gggtcatttt ttagtggctt ttgccaccca cgctttcagc acttctacgt
    46081 cgtgacgcca ctcttctttc atctcttcaa tccattcacc gacgttatct tcccaggcag
    46141 gaaggtccgg agactgaatt tgctgaccca gctgttgcag atggcgtaac cccactgacc
    46201 ccgccgcacc tttaatttta tgtccttcct ctacaatgcc ttttttatcc tgcgccgtca
    46261 gattcgactc cagcacgctg acatagcccg gcatcatctt ctcaaacacc gctaacccgt
    46321 cggtgatcag cttcggtcct acaagttcga gatactgttc cagcatggga atatcgagca
    46381 atgcttctga tttactgttc tcttctgtcg tcaccgtact ctcctcatca tcctgggtat
    46441 cccagaattt cttgatcatc gcggttagcg ccggaaccga aagcggctta ctcagcacat
    46501 catccattcc agcattgagg tactcttgtt tgtctttcag cacgttagcg gttaaggcca
    46561 ccagcggcgg taaatcctcg cgcggataac gcttcgtcag ttcacgagag atatccagcc
    46621 cggtcatatc tggcaactga atatccagca acaccaggtc gtattcgccc ggtttaaaca
    46681 tctccagcgc cgccttgccg gtcatggcga catcaacgct gttacctaat ttttccagca
    46741 cagaacgcgc aacaatcacg ttcagttcaa tgtcttccac cagcagcaca ttcagcgccg
    46801 gtaaaggcat atcgtcttca tcaaacgcat catcgacctc ttctgctacc gacggtgcgt
    46861 ggatcgtcaa cgtaaaggtt gaacctttgc cctgttcgct ggtaaccgta atatcgccgc
    46921 ccatattttt cgccagacga cgagaaacgg ccagaccaat accggtgccg gtggcaggtt
    46981 taccgccatg actgtctttc acctggtaat acatggcgaa aattttatcc agctcatcct
    47041 gcggaatgcc gataccagag tcttccactt caaaatgcag catatcgcct tcatcgtagc
    47101 gcacgcgcac ggtaacctgg ccttgctggg tgaatttgac ggcgttactg atgaggttcc
    47161 acaggatctg ccgtaaacgc gtcccgtcgg taatgacctg atgcggtaat ggcagcgtcg
    47221 gctccaggtt aaagcgcaat cctttttgtt gcgcctgcaa tgcggagaga ttttccagat
    47281 cggcaaggaa gctggtgaaa tcaaccggtt gattatcaag ctggaccttg cgccgttcca
    47341 tcttatccat gtcgataata tcgttaaaga tattccccag cgtgacggcc gaaacatgga
    47401 tggtcttgag atatttttcc tgctcggcgg tgagttcggt atccagcaga atacggctca
    47461 ggccgacgat accgttcagc ggtgtacgca attcgtgact gatggtggag ataaacgtcg
    47521 ttttgtcgcg gctggcccgt tcaagcgcat cctgataccg cttacgctcg gtaatgtcgc
    47581 gaccaaagcc catcaaaccg tgacgtttac ccacgcggtc gtagtacggc actttacgga
    47641 tttcaaagca ggctttgcgc ccgtccgggt aatccagcca ctgttcatag gtcagtgaca
    47701 cattatgacg gaacactttt tcatcggttt caatgacttt tgcggcggct tccggtgagt
    47761 aaacatcagc aggtttcagg tgaaccagtt gtttttcgct ttttccggtc agcagctcca
    47821 tcgcgcggtt acagccggaa aactctttat cttcgttacg ataaaaaacc aggtcgggtg
    47881 aagcatcaag gaaggaacgt aagaatgagg attgctgctc gagctgaatt tgtgtctctt
    47941 cgcgctcttt gatttcaatt ttcagttggc cgaaggtttc ctgcagttct gcttccgctt
    48001 tttcacgaac ggcgatttcc tgatttagct gggcaatatt atcttttaac tgaacgttga
    48061 ggctcaaatc gcgctcgcgc atctcctcca gtttttgcac cagccgtgac agacgttgtc
    48121 gtgactcctc cagttgctcg acgaccaccg atagaaagta gaccgcccac ggcgtaatca
    48181 gcaaaccaaa gaagatagaa cgaataacat caatgctttc gacctgacca tgcagcacca
    48241 tggttaccgc catttgtacc acaatggcaa gaacgacgag ggccagcgcc agcaacattg
    48301 agaagcgcac cagacctaac ttcatcatca ggtcaacata atactgcgcc agcagacgaa
    48361 tttgcttcat tagggaattc cttcacgaca acctgcccca ataataccca attctgacag
    48421 ttacgttata aattgtgtga gaaatgcgga attcccctca acgacctact ccgttggagg
    48481 tatccacgga cgttccagcg ccgatccctg tacgccatgt tcattcagat agcgatccag
    48541 ctcgaccatt cccgtccatc gattttcgca ccacagagga gcaagcagcg tcgggcgacg
    48601 ggcgctggcg gaaatgcggt ggtagatcac ttccggtggc gtatggcgaa tcatttctcc
    48661 ggcagtgagc gtgtaatcct ccagttcaat accgttcaaa cgccccgctt cccaggcttt
    48721 cgccataatg ctgcctttca caatatgcag cggatgcagc tttatgccgt ccacgccggt
    48781 ttcaaccact cgctccagcg tttgcaaaca ttcagcctgc ccttcgccag gcaggccgac
    48841 aattaagtgg gaacacacct tcagcccgcg ctgacgtgcc agctgggttg tacgctgata
    48901 acaggcaaaa tcatggccgc ggttgatgcg atgcagtgtt ttgtcgtggg cggtttgtag
    48961 ccccagctcc agccacactt cgtaaccctg gtccttatat tcgcaaagca gatccagcac
    49021 cgcatccggc acgcagtccg ggcgggtacc aacacacaaa ccgacaatat tggcctggct
    49081 caccgcctgc tgatacatag aacgcagcac ctgaacttcc gcaaaggtgc tggtatacgc
    49141 ctgaaagtag gccagatagc gtttagcgcg gttaactaaa ttcgcctggt gcgccagttg
    49201 ctcggcaatg gaacgatgct gctgcgcttc atcggcaaac gaggcaacat tacagaatgt
    49261 gcagccacca cgcccgatgg taccgtcacg gttagggcag ctaaaaccgc cgtgcagcgt
    49321 cagcttatgc accttttgcc cataacgacg ggtgagatca ccaccaaaca tattgactaa
    49381 tttctgtaac tgcataatct gatagaccgc gccttgaaaa gaggccaaag cctgccattt
    49441 ttagctcaat tcggcgatga cctggatcaa tcgtcccggc ctgcttttat caactgcata
    49501 atcaatcaaa attaccgaaa tttcatgcat aatcacataa atcacttttg cttatcttgt
    49561 gtcagatttt tttatctcct tatggatttt aggcaaaaac agtagcatga aacgtcatta
    49621 ccaattaagg cagtataaaa tgctggcttt ctcgtcagtt caaggcagga taagggttaa
    49681 cacgccttta tgacagtcag gaattgactg tttctctaac gacttccctt ttagccttaa
    49741 agataaaatc ta
//
LOCUS       DAZKHF020000005        49065 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2201S31241
            SAMN43267800-rid25106413.denovo.005, whole genome shotgun sequence.
ACCESSION   DAZKHF020000005 DAZKHF020000000
VERSION     DAZKHF020000005.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267800
            Sequence Read Archive: SRR30307263
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 49065)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 49065)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 49065)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350581.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350581.2
            Genome Coverage       :: 151x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:21:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,652
            CDSs (total)                      :: 5,557
            Genes (coding)                    :: 5,320
            CDSs (with protein)               :: 5,320
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 237
            CDSs (without protein)            :: 237
            Pseudo Genes (ambiguous residues) :: 0 of 237
            Pseudo Genes (frameshifted)       :: 102 of 237
            Pseudo Genes (incomplete)         :: 130 of 237
            Pseudo Genes (internal stop)      :: 52 of 237
            Pseudo Genes (multiple problems)  :: 41 of 237
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..49065
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267800-rid25106413.denovo.005"
                     /serotype="O 26:H11"
                     /isolate="2201S31241"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-01"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            48..971
                     /gene="ydcI"
                     /locus_tag="ACHOSC_RS01040"
                     /old_locus_tag="pgaptmp_000208"
     CDS             48..971
                     /gene="ydcI"
                     /locus_tag="ACHOSC_RS01040"
                     /old_locus_tag="pgaptmp_000208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310054.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR substrate-binding domain-containing
                     protein"
                     /protein_id="HGU4546222.1"
                     /translation="MEKNSLFSQRIRLRHLHTFVAVAQQGTLGRAAETLNLSQPALSK
                     TLNELEQLTGARLFERGRQGAQLTLPGEQFLTHAVRVLDAINTAGQSLHRKEGLNNDV
                     VRVGALPTAALGILPSVIGQFHQQQKETTLQVATMSNPMILAGLKTGEIDIGIGRMSD
                     PELMTGLNYELLFLESLKLVVRPNHPLLQENVTLSRVLEWPVVVSPEGTAPRQHSDAL
                     VQSQGCKIPSGCIETLSASLSRQLTVEYDYVWFVPSGAVKDDLRHATLVALPVPGHGA
                     GEPIGILTRVDATFSSGCQLMINAIRKSMPF"
     gene            complement(1009..2649)
                     /gene="trg"
                     /locus_tag="ACHOSC_RS01045"
                     /old_locus_tag="pgaptmp_000209"
     CDS             complement(1009..2649)
                     /gene="trg"
                     /locus_tag="ACHOSC_RS01045"
                     /old_locus_tag="pgaptmp_000209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein Trg"
                     /protein_id="HGU4546223.1"
                     /translation="MNTTPSHRLGFLHHIRLVPLFACILGGILVLFALSSALAGYFLW
                     QADRDQRDVTAEIEIRTGLANSSDFLRSARINMIQAGAASRIAEMEAMKRNIAQAESE
                     IKQSQQGYRAYQNRPVKTPADEALDTELNQRFQAYITGMQPMLKYAKNGMFEAIINHE
                     SEQIRPLDNAYTDILNKAVKIRSTRANQLAELAHQRTRLGGMFMIGAFVLALVMTLIT
                     FMVLRRIVIRPLQHAAQRIEKIASGDLTMNDEPAGRNEIGRLSRHLQQMQHSLGMTVG
                     TVRQGAEEIYRGTSEISAGNADLSSRTEEQAAAIEQTAASMEQLTATVKQNADNAHHA
                     SKLAQEASIKASDGGQTVSGVVKTMGAISTSSKKISEITAVINSIAFQTNILALNAAV
                     EAARAGEQGRGFAVVASEVRTLASRSAQAAKEIEGLISESVRLIDLGSDEVATAGKTM
                     STIVDAVASVTHIMQEIAAASDEQSRGITQVSQAISEMDKVTQQNASLVEEASAAAVS
                     LEEQAARLTEAVDVFRLHKHSVSAEPRGAGEPVSFATV"
     gene            3048..3197
                     /gene="hokB"
                     /locus_tag="ACHOSC_RS01050"
                     /old_locus_tag="pgaptmp_000210"
     CDS             3048..3197
                     /gene="hokB"
                     /locus_tag="ACHOSC_RS01050"
                     /old_locus_tag="pgaptmp_000210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_025301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin HokB"
                     /protein_id="HGU4546224.1"
                     /translation="MKHNPLVVCLLIICITILTFTLLTRQTLYELRFRDGDKEVAALM
                     ACTSR"
     gene            complement(3175..3243)
                     /gene="yncP"
                     /locus_tag="ACHOSC_RS01055"
                     /old_locus_tag="pgaptmp_000211"
     CDS             complement(3175..3243)
                     /gene="yncP"
                     /locus_tag="ACHOSC_RS01055"
                     /old_locus_tag="pgaptmp_000211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YncP"
                     /protein_id="HGU4546225.1"
                     /translation="MNLLVRCAGKIPALALTWTCRP"
     gene            complement(3269..3442)
                     /locus_tag="ACHOSC_RS01060"
                     /old_locus_tag="pgaptmp_000212"
     CDS             complement(3269..3442)
                     /locus_tag="ACHOSC_RS01060"
                     /old_locus_tag="pgaptmp_000212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4546226.1"
                     /translation="MKKLALILFMGTLVSFYADAGRKPCSGSKGGISHCTAGGKFVCN
                     DGSISASKKTCTN"
     gene            complement(3687..4217)
                     /gene="cybB"
                     /locus_tag="ACHOSC_RS01065"
                     /old_locus_tag="pgaptmp_000213"
     CDS             complement(3687..4217)
                     /gene="cybB"
                     /locus_tag="ACHOSC_RS01065"
                     /old_locus_tag="pgaptmp_000213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415935.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_process: GO:0022904 - respiratory electron transport
                     chain [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome b561"
                     /protein_id="HGU4546227.1"
                     /translation="MENKYSRLQISIHWLVFLLVIAAYCAMEFRGFFPRSDRPLINMV
                     HVSCGISILVLMVVRLLLRLKYPTPPIIPKPKPMMTGLAHLGHLVIYLLFIALPVIGL
                     VMMYNRGNPWFAFGLTMPYASEANFERVDSLKSWHETLANLGYFVIGLHAAAALAHHY
                     FWKDNTLLRMMPRKRS"
     gene            4406..5407
                     /gene="gap"
                     /locus_tag="ACHOSC_RS01070"
                     /old_locus_tag="pgaptmp_000214"
     CDS             4406..5407
                     /gene="gap"
                     /locus_tag="ACHOSC_RS01070"
                     /old_locus_tag="pgaptmp_000214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0006006 - glucose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HGU4546228.1"
                     /translation="MSKVGINGFGRIGRLVLRRLLEVKSNIDVVAINDLTSPKILAYL
                     LKHDSNYGPFLWSVDFTEDSLIVDGKSIAVYAEKEAKNIPWKAKGAEIIVECTGFYTS
                     AEKSQAHLDAGAKKVLISAPAGEMKTIVYNVNDDTLDGNDTIVSVASCTTNCLAPMAK
                     ALHDSFGIEVGTMTTIHAYTGTQSLVDGPRGKDLRASRAAAENIIPHTTGAAKAIGLV
                     IPELSGKLKGHAQRVPVKTGSVTELVSILGKKVTAEEVNNALKQATTNNESFGYTDEE
                     IVSSDIIGSHFGSVFDATQTEITAVGDLQLVKTVAWYDNEYGFVTQLIRTLEKFAKL"
     gene            complement(5449..6888)
                     /gene="aldA"
                     /locus_tag="ACHOSC_RS01075"
                     /old_locus_tag="pgaptmp_000215"
     CDS             complement(5449..6888)
                     /gene="aldA"
                     /locus_tag="ACHOSC_RS01075"
                     /old_locus_tag="pgaptmp_000215"
                     /EC_number="1.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase"
                     /protein_id="HGU4546229.1"
                     /translation="MSVPVQHPMYIDGQFVTWRGDAWIDVVNPAAEAVISRIPDGQAE
                     DARKAIDAAERAQPEWEALPAIERASWLRKISAGIRERASEISALIVEEGGKIQQLAE
                     VEVAFTADYIDYMAEWARRYEGEIIQSDRPGENILLFKRALGVTTGILPWNFPFFLIA
                     RKMAPALLTGNTIVIKPSEFTPNNAIAFAKIVDEIGLPRGVFNLVLGRGETVGQELAG
                     NPKVAMVSMTGSVSAGEKIMATAAKNITKVCLELGGKAPAIVMDDADLELAVKAIVDS
                     RVINSGQVCNCAERVYVQKGIYDQFVNRLGEAMQAVQFGNPAERNDIAMGPLINAAAL
                     ERVEQKVARAVEEGARVALGGKAVDGKGYYYPPTLLLDVRQEMSIMHEETFGPVLPVV
                     AFDTLEDAISMANDSDYGLTSSIYTQNLNVAMKAIKGLKFGETYINRENFEAMQGFHA
                     GWRKSGIGGADGKHGLHEYLQTQVVYLQS"
     gene            complement(7085..7885)
                     /gene="ydcF"
                     /locus_tag="ACHOSC_RS01080"
                     /old_locus_tag="pgaptmp_000216"
     CDS             complement(7085..7885)
                     /gene="ydcF"
                     /locus_tag="ACHOSC_RS01080"
                     /old_locus_tag="pgaptmp_000216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF218 domain-containing protein YdcF"
                     /protein_id="HGU4546230.1"
                     /translation="MNITPFPTLSPATIDAINVIGQWLAQDDFSGEVPYQADCVILAG
                     NAVMPTIDAACKIARDQQIPLLISGGIGHSTTFLYSAIAQHPHYNTIRTTGRAEATIL
                     ADIAHQFWHIPHEKIWIEDQSTNCGENARFSIALLNQAVERVHTAIVVQDPTMQRRTM
                     ATFRRMTGDNPDAPRWLSYPGFVPQLGNNADSVIFINQLQGLWPVERYLSLLTGELPR
                     LRDDSDGYGPRGRDFIVHVDFPAEVIHAWQTLKHDAVLIEAMESRSLR"
     gene            complement(8157..12059)
                     /gene="hrpA"
                     /locus_tag="ACHOSC_RS01085"
                     /old_locus_tag="pgaptmp_000217"
     CDS             complement(8157..12059)
                     /gene="hrpA"
                     /locus_tag="ACHOSC_RS01085"
                     /old_locus_tag="pgaptmp_000217"
                     /EC_number="3.6.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415931.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003724 - RNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RNA helicase HrpA"
                     /protein_id="HGU4546231.1"
                     /translation="MTEQQKLTFTALQQRLDSLMLRDRLRFSRRLHGVKKVKNPDAQQ
                     AIFQEMAKEIDQAAGKVLLREAARPEITYPDNLPVSQKKQDILEAIRDHQVVIVAGET
                     GSGKTTQLPKICMELGRGIKGLIGHTQPRRLAARTVANRIAEELKTEPGGCIGYKVRF
                     SDHVSDNTMVKLMTDGILLAEIQQDRLLMQYDTIIIDEAHERSLNIDFLLGYLKELLP
                     RRPDLKIIITSATIDPERFSRHFNNAPIIEVSGRTYPVEVRYRPIVEEADDTERDQLQ
                     AIFDAVDELSQESPGDILIFMSGEREIRDTADALNKLNLRHTEILPLYARLSNSEQNR
                     VFQSHSGRRIVLATNVAETSLTVPGIKYVIDPGTARISRYSYRTKVQRLPIEPISQAS
                     ANQRKGRCGRVSEGICIRLYSEDDFLSRPEFTDPEILRTNLASVILLMTALGLGDIAA
                     FPFVEAPDKRNIQDGVRLLEELGAITTDEQASAYKLTPLGRQLSQLPVDPRLARMVLE
                     AQKHGCVREAMIITSALSIQDPRERPMDKQQASDEKHRRFHDKESDFLAFVNLWNYLG
                     EQQKALSSNAFRRLCRIDYLNYLRVREWQDIYTQLRQVVKELGIPVNSEPAEYREIHI
                     ALLTGLLSHIGMKDADKQEYTGARNARFSIFPGSGLFKKPPKWVMVAELVETSRLWGR
                     IAARIDPEWVEPVAQHLIKRTYSEPHWERAQGAVMATEKVTVYGLPIVAARKVNYSQI
                     DPALCRELFIRHALVEGDWQTRHAFFRENLKLRAEVEELEHKSRRRDILVDDETLFEF
                     YDQRISHDVISARHFDSWWKKVSRETPDLLNFEKSMLIKEGAEKISKLDYPNFWHQGN
                     LKLRLSYQFEPGADADGVTVHIPLPLLNQVEESGFEWQIPGLRRELVIALIKSLPKPV
                     RRNFVPAPNYAEAFLGRVKPLELPLLDSLERELRRMTGVTVDREDWHWDQVPDHLKIT
                     FRVVDDKNKKLKEGRSLQDLKDALKGKVQETLSAVADDGIEQSGLHIWSFGQLPESYE
                     QKRGNYKVKAWPALVDERDSVAIKLFDNPLEQKQAMWNGLRRLLLLNIPSPIKYLHEK
                     LPNKAKLGLYFNPYGKVLELIDDCISCGVDQLIDANGGPVWTEEGFAALHEKVRAELN
                     DTVVDIAKQVEQILTAVFNINKRLKGRVDMTMALGLSDIKAQMGGLVYRGFVTGNGFK
                     RLGDTLRYLQAIEKRLEKLAVDPHRDRAQMLKVENVQQAWQQWINKLPPARREDEDVK
                     EIRWMIEELRVSYFAQQLGTPYPISDKRILQAMEQISG"
     gene            12260..12865
                     /gene="azoR"
                     /locus_tag="ACHOSC_RS01090"
                     /old_locus_tag="pgaptmp_000218"
     CDS             12260..12865
                     /gene="azoR"
                     /locus_tag="ACHOSC_RS01090"
                     /old_locus_tag="pgaptmp_000218"
                     /EC_number="1.7.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FMN-dependent NADH-azoreductase"
                     /protein_id="HGU4546232.1"
                     /translation="MSKVLVLKSSILAGYSQSNQLSDYFVEQWREKHSADEITVRDLA
                     ANPIPVLDGELVGALRPSDAPLTPRQQEALALSDELIAELKAHDVIVIAVPMYNFNIS
                     TQLKNYFDLVARAGVTFRYTENGPEGLVTGKKAIVITSRGGIHKDGPTDLVTPYLSTF
                     LGFIGITDVKFVFAEGIAYGPEMAAKAQSDAKAAIDSIVAA"
     gene            complement(12916..14232)
                     /gene="ynbD"
                     /locus_tag="ACHOSC_RS01095"
                     /old_locus_tag="pgaptmp_000219"
     CDS             complement(12916..14232)
                     /gene="ynbD"
                     /locus_tag="ACHOSC_RS01095"
                     /old_locus_tag="pgaptmp_000219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310040.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2/dual specificity phosphatase
                     family protein"
                     /protein_id="HGU4546233.1"
                     /translation="MITERRNVLLQGAGWLLLLAPFFFFTYGSLNQFTAVQDLNSHDI
                     PSQVFGWETAIPFLPWTIVPYWSLDLLYGFSLFVCSTTFEQRRLVHRLILATVMACCG
                     FLLYPLKFSFIRPEVSGVTGWLFSQLELFDLPYNQSPSLHIILCWLLWRHFRQHLAER
                     WRKVCGGWFLLIAISTLTTWQHHFIDVITGLAVGMLIDWMVPVDRRWNYQKPDQRRIK
                     IALPYVVGAGSCIVLMELMVMIQLWWSVWLCWPVLSLLIIGRGYGGLGAITTGKDSQG
                     KLPPAVYWLTLPWRIGMWLSMRWFCRRLEPVSKITAGVYLGAFPRHIPAQNAVLDVTF
                     EFPRGRATKDRLYFCVPMLDLVVPEEGELRQAVAMLETLREEQGSVLVHCALGLSRSA
                     LVVAAWLLCYGHCKTVDEAISYIRARRSHIVLKEDHKAMLKLWENR"
     gene            complement(14222..15979)
                     /gene="ynbC"
                     /locus_tag="ACHOSC_RS01100"
                     /old_locus_tag="pgaptmp_000220"
     CDS             complement(14222..15979)
                     /gene="ynbC"
                     /locus_tag="ACHOSC_RS01100"
                     /old_locus_tag="pgaptmp_000220"
                     /EC_number="2.1.1.-"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional alpha/beta hydrolase/class I
                     SAM-dependent methyltransferase"
                     /protein_id="HGU4546234.1"
                     /translation="MENSRIHGEHFFTSSDNTALFYRHWPALQPGAKKVIVLFHRGHE
                     HSGRLQHLVDELAMPDTVFYAWDARGHGQTSGPRGYSPSLARSVQDVDEFVRFAASDS
                     QVGLDEVVVIAQSVGAVLVATWVHDYAPAIRGLVLASPAFKVKLYVPLARPALALWHR
                     LRGLFFINSYVKGRYLTHDRQRVASFNNDPLITRAIAVNILLDLYKTSERIIRDAAAI
                     TLPTQLLISGDDYVVHRQPQIDFYQRLRSPLKELHLLPGFYHDTLGEENRAQAFEKMQ
                     SFISRLYANKSQKFDYQHEDRTGPSADRWRLLSGGPVPLSPVDLAYRFMRKAMKLFGA
                     HSAGLHLGMSTGFDSGSSLDYVYQNQPQGSNAFGRLIDKIYLNSVGWRGIRQRKTHLQ
                     ILIKQAVADLHAKGLAVRVVDIAAGHGRYVLDALANEPAVSDILLRDYSELNVAQGQA
                     MIAQRGMSGRVRFEQGDAFNPEELSALTPRPTLAIVSGLYELFPENEQVKNSLAGLAN
                     AIEPGGILIYTGQPWHPQLEMIAGVLTSHKDGKPWVMRVRSQGEMDSLVRDAGFDKCT
                     QRIDEWGIFTVSMAVRRDN"
     gene            complement(15995..16891)
                     /gene="ynbB"
                     /locus_tag="ACHOSC_RS01105"
                     /old_locus_tag="pgaptmp_000221"
     CDS             complement(15995..16891)
                     /gene="ynbB"
                     /locus_tag="ACHOSC_RS01105"
                     /old_locus_tag="pgaptmp_000221"
                     /EC_number="2.7.7.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004605 - phosphatidate
                     cytidylyltransferase activity [Evidence IEA];
                     GO_process: GO:0016024 - CDP-diacylglycerol biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidate cytidylyltransferase"
                     /protein_id="HGU4546235.1"
                     /translation="MLEKSLATLFALLILATLINHFLLWRLPERKEDEVTLRIRTWWG
                     IVICFSLVISGPRWMTLTFFALISFLALKEYCTLISVHFPRWLYWVIPLNYLFIGFNC
                     FELYLLFIPLAGFLILATGQVFMGDPSGFLHTVSAIFWGWIMTVFALSHAAWLLMLPT
                     TNIQGGALLVLFLLALTESNDIAQYLWGKSCGRRKVVPKVSPGKTLEGLVGGVITTMI
                     ASLIIGPLLTPLNTLQALLAGLLIGISGFCGDVVMSAIKRDIGVKDSGKLLPGHGGLL
                     DRIDSLIFTAPVFFYFIRYCCY"
     gene            complement(16891..17496)
                     /gene="ynbA"
                     /locus_tag="ACHOSC_RS01110"
                     /old_locus_tag="pgaptmp_000222"
     CDS             complement(16891..17496)
                     /gene="ynbA"
                     /locus_tag="ACHOSC_RS01110"
                     /old_locus_tag="pgaptmp_000222"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310037.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0016780 - phosphotransferase activity, for
                     other substituted phosphate groups [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-alcohol phosphatidyltransferase family
                     protein"
                     /protein_id="HGU4546236.1"
                     /translation="MTLYQIKPLFQSLLRPTMFWLYKHHVTANHITLAALALSLLTGL
                     LLMLVAQPILFMLLPIVLFIRMALNALDGMLARECNQQTRLGAILNETGDVISDIALY
                     LPFLFLPESNASLVILMLFCTILTEFCGLLAQTINGVRSYAGPFGKSDRALIFGLWGL
                     AVAIYPQWMQWNNLLWSIASILLLWTAINRCRSVLLMSAER"
     gene            complement(17667..19973)
                     /gene="ydbD"
                     /locus_tag="ACHOSC_RS01115"
                     /old_locus_tag="pgaptmp_000223"
     CDS             complement(17667..19973)
                     /gene="ydbD"
                     /locus_tag="ACHOSC_RS01115"
                     /old_locus_tag="pgaptmp_000223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415925.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2773 domain-containing bactofilin"
                     /protein_id="HGU4546237.1"
                     /translation="MFKSRICGWIGLLPLFMLSLPVQAELRCVANTVDIEPFFSAATA
                     EDKQQVEQAINSSVNLVPFGLSASDWKVHRGDLVVEGNIESNQKLIVLGNLTVKGNIS
                     TFSLSNPWVILGNVTATNIVTDSPLLITGSINASGLVFIDSYYDNPSSIKGSINARGI
                     FINDIIAPVVASSTNSEFMVRASDKNDTENVKKALMIINPDAYYWGLINDEDALKEIF
                     KRSNIRMAGNVCNQMKKEALFRPKPSPELVQELQMLDEGNVAAFEGRDIATFDLAIMR
                     TLPRLKGISANLRKQLINSNDEQTIESMARYMPDNEILELTDQQLGYQPVVLGLLDRE
                     PLSVEIMTRMSRLPDGVGPLNLALRENLPLDIVMTLAKRDWDMIIQELYKDAWLLPES
                     IIDGYIRSDDSSIRQVGAGGQLTYNQAMQLANDSSNNVVTSLAFKLAEMKHHGQLLRM
                     TPQESDKVAAYLYQKFENDDDLIRVLFLALPDNLQFNFVKRMEKKSPAYFCCRDMQVI
                     HSDAALQRLLTRFNDPEGWSNLAKNQYLSTSMKQKIWQRALSHRKNNPKADSAAYETS
                     ADMILSELISHGEVDDQMLLNATALIRLEDWDFLESALVSWDNLPAVVLKELQQNTPR
                     NDIWAKFFLRQENSSRAQVDEALRVYYALDPDALAQLDVLAKQPDRIWWSTLAKSNLT
                     FFKFGALNNRHTPPAVLAAEIDPEWWIVAMNNPRFPVDVLKARLKRDPLLALELVNPE
                     LDLVRQLALNGKTRAIREQAMRKLDELY"
     gene            complement(20037..20897)
                     /gene="pdxI"
                     /locus_tag="ACHOSC_RS01120"
                     /old_locus_tag="pgaptmp_000224"
     CDS             complement(20037..20897)
                     /gene="pdxI"
                     /locus_tag="ACHOSC_RS01120"
                     /old_locus_tag="pgaptmp_000224"
                     /EC_number="1.1.1.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxine 4-dehydrogenase"
                     /protein_id="HGU4546238.1"
                     /translation="MSSNTFTLGTKSVNRLGYGAMQLAGPGVFGPPRDRHVAITVLRE
                     ALALGVNHIDTSDFYGPHVTNQIIREALYPYSDDLTIVTKIGARRGEDASWLPAFSPA
                     ELQKAVHDNLRNLGLDVLDVVNLRVMMGDGHGPAEGSIEASLTVLAEMQQQGLVKHIG
                     LSNVTPTQVAEARKIAEIVCVQNEYNIAHRADDAMIDALAHDGIAYVPFFPLGGFTPL
                     QSSTLSDVAASLGATPMQVALAWLLQRSPNILLIPGTSSVAHLRENMAAEKLHLSEEV
                     LSTLDGISRE"
     gene            complement(21128..21718)
                     /gene="paaY"
                     /locus_tag="ACHOSC_RS01125"
                     /old_locus_tag="pgaptmp_000225"
     CDS             complement(21128..21718)
                     /gene="paaY"
                     /locus_tag="ACHOSC_RS01125"
                     /old_locus_tag="pgaptmp_000225"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0010124 - phenylacetate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylacetic acid degradation protein PaaY"
                     /protein_id="HGU4546239.1"
                     /translation="MPIYQIDGLTPVVPEESFVHPTAVLIGDVILGKGVYVGPNASLR
                     GDFGRIVVKDGANIQDNCVMHGFPEQDTVVEEDGHIGHSAILHGCIIRRNALVGMNAV
                     VMDGAVIGENSIVGASAFVKAKAEMPANYLIVGSPAKAIRELSEQELAWKKQGTHEYQ
                     VLVTRCKQTLHQVEPLREVEPNRKRLVFDENLRPKQ"
     gene            complement(21700..22650)
                     /gene="paaX"
                     /locus_tag="ACHOSC_RS01130"
                     /old_locus_tag="pgaptmp_000226"
     CDS             complement(21700..22650)
                     /gene="paaX"
                     /locus_tag="ACHOSC_RS01130"
                     /old_locus_tag="pgaptmp_000226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylacetic acid degradation operon negative
                     regulatory protein PaaX"
                     /protein_id="HGU4546240.1"
                     /translation="MSKLVTFIQHAVNAVPVSGTSLISSLYGDSLSHRGGEIWLGSLA
                     ALLEGLGFGERFVRTALFRLNKEGWLDVSRIGRRSFYSLSDKGLRLTRRAESKIYRAE
                     QPAWDGKWLLLLSEGLDKSTLADVKKQLIWQGFGALAPSLMASPSQKLADVQTLLHEA
                     GVADNVICFEAQIPLALSRAALRARVEECWHLTEQNAMYETFIQSFRPLVPLLKEAAD
                     ELTPERAFHIQLLLIHFYRRVVLKDPLLPEELLPAHWAGHTARQLCINIYQRVAPAAL
                     AFVSEKGETSVGELPAPGSLYFQRFGGLNIEQEAICQFTR"
     gene            complement(22751..24064)
                     /gene="paaK"
                     /locus_tag="ACHOSC_RS01135"
                     /old_locus_tag="pgaptmp_000227"
     CDS             complement(22751..24064)
                     /gene="paaK"
                     /locus_tag="ACHOSC_RS01135"
                     /old_locus_tag="pgaptmp_000227"
                     /EC_number="6.2.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047475 - phenylacetate-CoA ligase
                     activity [Evidence IEA];
                     GO_process: GO:0010124 - phenylacetate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylacetate--CoA ligase PaaK"
                     /protein_id="HGU4546241.1"
                     /translation="MITNTKLDPIETASVDELQALQTQRLKWTLKHAYENVPMYRRKF
                     DAAGVHPDDFRELSDLRKFPCTTKQDLRDNYPFDTFAVPMEQVVRIHASSGTTGKPTV
                     VGYTQNDIDNWANIVARSLRAAGGTPKDKIHVAYGYGLFTGGLGAHYGAERLGATVIP
                     MSGGQTEKQAQLIRDFQPDMIMVTPSYCLNLIEELERQLGGDASGCSLRVGVFGAEPW
                     TQAMRKEIERRLGITALDIYGLSEVMGPGVAMECLETTDGPTIWEDHFYPEIVNPHDG
                     TPLADGEHGELLFTTLTKEALPVIRYRTRDLTRLLPGTARTMRRMDRISGRSDDMLII
                     RGVNVFPSQLEEEIVKFEHLSPHYQLEVNRRGHLDSLSVKVELKESSLTLTHEQRCQV
                     CHQLRHRIKSMVGISTDVMIVNCGSIPRSEGKACRVFDLRNIVGA"
     gene            complement(24091..25296)
                     /gene="paaJ"
                     /locus_tag="ACHOSC_RS01140"
                     /old_locus_tag="pgaptmp_000228"
                     /gene_synonym="paaE"
                     /gene_synonym="ydbW"
     CDS             complement(24091..25296)
                     /gene="paaJ"
                     /locus_tag="ACHOSC_RS01140"
                     /old_locus_tag="pgaptmp_000228"
                     /gene_synonym="paaE"
                     /gene_synonym="ydbW"
                     /EC_number="2.3.1.174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     3-oxoadipyl-CoA/3-oxo-5,6-dehydrosuberyl-CoA thiolase"
                     /protein_id="HGU4546242.1"
                     /translation="MREAFICDGIRTPIGRYGGALSGVRADDLAAIPLRELLVRNPRL
                     DAECIDDVILGCANQAGEDNRNVARMATLLAGLPHSVSGTTINRLCGSGLDALGFAAR
                     AIKAGDGDLLIAGGVESMSRAPFVMGKATSAFSRQAEMFDTTIGWRFVNPLMAQQFGT
                     DSMPETAENVAELLKISREDQDSFALRSQQRTAKAQSSGILAEEIVPVVLKNKKGVVT
                     EIQHDEHLRPETTLEQLRGLKAPFRANGVITAGNASGVNDGAAALIIASEQMAAAQGL
                     TPRARIVAMATAGVEPRLMGLGPVPATRRVLERAGLSIHDMDVIELNEAFAAQALGVL
                     RELGLPDDAPHVNPNGGAIALGHPLGMSGARLALAASHELHRRNGRYALCTMCIGVGQ
                     GIAMILERV"
     gene            complement(25296..25718)
                     /gene="paaI"
                     /locus_tag="ACHOSC_RS01145"
                     /old_locus_tag="pgaptmp_000229"
     CDS             complement(25296..25718)
                     /gene="paaI"
                     /locus_tag="ACHOSC_RS01145"
                     /old_locus_tag="pgaptmp_000229"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016790 - thiolester hydrolase activity
                     [Evidence IEA];
                     GO_process: GO:0010124 - phenylacetate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyphenylacetyl-CoA thioesterase PaaI"
                     /protein_id="HGU4546243.1"
                     /translation="MSHKAWQNAHAMYENDACAKALGIDIISMDEGFAVVTMTVTAQM
                     LNGHQSCHGGQLFSLADTAFAYACNSQGLAAVASACTIDFLRPGFAGDTLTATAQVRH
                     QGKQTGVYDIEIVNQQQKTVALFRGKSHRIGGTITGEA"
     gene            complement(25708..27135)
                     /gene="paaH"
                     /locus_tag="ACHOSC_RS01150"
                     /old_locus_tag="pgaptmp_000230"
     CDS             complement(25708..27135)
                     /gene="paaH"
                     /locus_tag="ACHOSC_RS01150"
                     /old_locus_tag="pgaptmp_000230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008691 - 3-hydroxybutyryl-CoA
                     dehydrogenase activity [Evidence IEA];
                     GO_process: GO:0010124 - phenylacetate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-CoA dehydrogenase PaaH"
                     /protein_id="HGU4546244.1"
                     /translation="MMINVQTVAVIGSGTMGAGIAEVAASHGHQVLLYDISAEALTRA
                     IDGIHARLNSRVTRGKLTAETCERTLKRLIPVTDIHALAAADLVIEAASERLEVKKAL
                     FAQLAEVCPPQTLLTTNTSSISITAIAAEVKNPERVAGLHFFNPAPVMKLVEVVSGLA
                     TAAEVVEQLCELTLSWGKQPVRCHSTPGFIVNRVARPYYSEAWRALEEQVAAPEVIDA
                     ALRDGAGFPMGPLELTDLIGQDVNFAVTCSVFNAFWQERRFLPSLVQQELVIGGRLGK
                     KSGLGVYDWRAEREAVVGLEAVSDSFSPMKVEKKSDGVTEIDDVLLIETQGETAQALA
                     IRLARPVVVVDKMAGKVVTIAAAAVNPDSATRKAIYYLQQQGKTVLQIADYPGMLIWR
                     TVAMIINEALDALQKGVASEQDIDTAMRLGVNYPYGPLAWGAQLGWQRILRLLENLQH
                     HYGEERYRPCSLLRQRALLESGYES"
     gene            complement(27137..27925)
                     /gene="paaG"
                     /locus_tag="ACHOSC_RS01155"
                     /old_locus_tag="pgaptmp_000231"
     CDS             complement(27137..27925)
                     /gene="paaG"
                     /locus_tag="ACHOSC_RS01155"
                     /old_locus_tag="pgaptmp_000231"
                     /EC_number="5.3.3.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA];
                     GO_process: GO:0010124 - phenylacetate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-(1,2-epoxy-1,2-dihydrophenyl)acetyl-CoA
                     isomerase PaaG"
                     /protein_id="HGU4546245.1"
                     /translation="MMEFILSHVEKGVMTLTLNRPERLNSFNDEMHAQLAECLKQVER
                     DDTIRCLLLTGAGRGFCAGQDLNDRNVDPTGPAPDLGMSVERFYNPLVRRLAKLPKPV
                     ICAVNGVAAGAGATLALGCDIVIAARSAKFVMAFSKLGLIPDCGGTWLLPRVAGRARA
                     MGLALLGNQLSAEQAHEWGMIWQVVDDETLADTAQQLARHLATQPTFGLGLIKQAINS
                     AETNTLDTQLDLERDYQRLAGRSADYREGVSAFLAKRSPQFTGK"
     gene            complement(27925..28692)
                     /gene="paaF"
                     /locus_tag="ACHOSC_RS01160"
                     /old_locus_tag="pgaptmp_000232"
     CDS             complement(27925..28692)
                     /gene="paaF"
                     /locus_tag="ACHOSC_RS01160"
                     /old_locus_tag="pgaptmp_000232"
                     /EC_number="4.2.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-dehydroadipyl-CoA hydratase PaaF"
                     /protein_id="HGU4546246.1"
                     /translation="MSELIVSRQQRVLLLTLNRPAARNALNNALLMQLVNELEAAATD
                     TSISVCVITGNARFFAAGADLNEMAEKDLAATLNDTRPQLWARLQAFNKPLIAAVNGY
                     ALGAGCELALLCDVVVAGENARFGLPEITLGIMPGAGGTQRLIRSVGKSLASKMVLSG
                     ESITAQQAQQAGLVSDVFPSDLTLEYALQLASKMARHSPLALQAAKQALRQSQEVALQ
                     AGLAQERQLFTLLAATEDRHEGISAFLQKRTPDFKGR"
     gene            complement(28689..29759)
                     /gene="paaE"
                     /locus_tag="ACHOSC_RS01165"
                     /old_locus_tag="pgaptmp_000233"
     CDS             complement(28689..29759)
                     /gene="paaE"
                     /locus_tag="ACHOSC_RS01165"
                     /old_locus_tag="pgaptmp_000233"
                     /EC_number="1.14.13.149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0010124 - phenylacetate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1,2-phenylacetyl-CoA epoxidase subunit PaaE"
                     /protein_id="HGU4546247.1"
                     /translation="MTTFHSLTVAKVEPETRDAVTITFAVPQPLQEAYRFRPGQHLTL
                     KASLDGEELRRCYSICRSYLPGEISVAVKAIEGGRFSRYAREHIRQGMTLEVMVPQGH
                     FGYQPQAERQGRYLAIAAGSGITPMLAIIATTLQTEPESQFTLIYGNRTSQSMMFRQA
                     LADLKDKYPQRLQLLCIFSQETLDSDLLHGRIDGERLQSLGASLINFRLYDEAFICGP
                     AAMMDETEAALKALGMPDKTIHLERFNTPGTRVKRSVNVQSDGQKVTVRQDGRDREIV
                     LNADDESILDAALRQGADLPYACKGGVCATCKCKVLRGKVAMETNYSLEPDELAAGYV
                     LSCQALPLTSDVVVDFDAKGMA"
     gene            complement(29767..30264)
                     /gene="paaD"
                     /locus_tag="ACHOSC_RS01170"
                     /old_locus_tag="pgaptmp_000234"
     CDS             complement(29767..30264)
                     /gene="paaD"
                     /locus_tag="ACHOSC_RS01170"
                     /old_locus_tag="pgaptmp_000234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415909.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0010124 - phenylacetate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1,2-phenylacetyl-CoA epoxidase subunit PaaD"
                     /protein_id="HGU4546248.1"
                     /translation="MQRLATIAPPQVHEIWALLSQIPDPEIPVLTITDLGMVRNVTQM
                     GEGWVIGFTPTYSGCPATEHLIGAIREAMTTHGFTPVQVVLQLDPAWTTDWMTPDARE
                     RLRQYGISPPAGHSCHAHLPPEVRCPRCASVHTTLISEFGSTACKALYRCDSCREPFD
                     YFKCI"
     gene            complement(30279..31025)
                     /gene="paaC"
                     /locus_tag="ACHOSC_RS01175"
                     /old_locus_tag="pgaptmp_000235"
     CDS             complement(30279..31025)
                     /gene="paaC"
                     /locus_tag="ACHOSC_RS01175"
                     /old_locus_tag="pgaptmp_000235"
                     /EC_number="1.14.13.149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0010124 - phenylacetate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1,2-phenylacetyl-CoA epoxidase subunit PaaC"
                     /protein_id="HGU4546249.1"
                     /translation="MNQLTAYTLRLGDNYLVLSQRLGEWCGHAPELEIDLALANIGLD
                     LLGQARNFLSYAAELAGEGDEDTLAFTRDERQFSNLLLVEQPNGNFADTIARQYFIDA
                     WHVALFTRLMESRDPQLAAISAKAIKEARYHLRFSRGWLERLGNGTDVSGQKMQQAID
                     KLWRFTAELFDADEIDIALSEEGIAVDPRTLRAAWEAEVFAGINEATLNVPQEQAYRT
                     GGKKGLHTEHLGPMLAEMQYLQRVLPGQQW"
     gene            complement(31034..31321)
                     /gene="paaB"
                     /locus_tag="ACHOSC_RS01180"
                     /old_locus_tag="pgaptmp_000236"
     CDS             complement(31034..31321)
                     /gene="paaB"
                     /locus_tag="ACHOSC_RS01180"
                     /old_locus_tag="pgaptmp_000236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0010124 - phenylacetate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1,2-phenylacetyl-CoA epoxidase subunit PaaB"
                     /protein_id="HGU4546250.1"
                     /translation="MSNVYWPLYEVFVRGKQGLSHRHVGSLHAADERMALENARDAYT
                     RRSEGCSIWVVKASEIVASQPEERGEFFDPAESKVYRHPTFYTIPDGIEHM"
     gene            complement(31333..32262)
                     /gene="paaA"
                     /locus_tag="ACHOSC_RS01185"
                     /old_locus_tag="pgaptmp_000237"
     CDS             complement(31333..32262)
                     /gene="paaA"
                     /locus_tag="ACHOSC_RS01185"
                     /old_locus_tag="pgaptmp_000237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0097266 - phenylacetyl-CoA 1,2-epoxidase
                     activity [Evidence IEA];
                     GO_process: GO:0010124 - phenylacetate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1,2-phenylacetyl-CoA epoxidase subunit PaaA"
                     /protein_id="HGU4546251.1"
                     /translation="MTQEERFEQRIAQETAIEPQDWMPDAYRKTLIRQIGQHAHSEIV
                     GMLPEGNWITRAPTLRRKAILLAKVQDEAGHGLYLYSAAETLGCAREDIYQKMLDGRM
                     KYSSIFNYPTLSWADIGVIGWLVDGAAIVNQVALCRTSYGPYARAMVKICKEESFHQR
                     QGFEACMALAQGSEAQKQMLQDAINRFWWPALMMFGPNDDNSPNSARSLAWKIKRFTN
                     DELRQRFVDNTVPQVEMLGMTVPDPDLHFDTESGHYRFGEIDWQEFNEVINGRGICNQ
                     ERLDAKRKAWEEGTWVREAALAHAQKQLARKVA"
     gene            32547..34592
                     /gene="paaZ"
                     /locus_tag="ACHOSC_RS01190"
                     /old_locus_tag="pgaptmp_000238"
     CDS             32547..34592
                     /gene="paaZ"
                     /locus_tag="ACHOSC_RS01190"
                     /old_locus_tag="pgaptmp_000238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylacetic acid degradation bifunctional
                     protein PaaZ"
                     /protein_id="HGU4546252.1"
                     /translation="MQQLASFLSGTWQSGRGRSRLIHHAISGEALWEVTSEGLDMAAA
                     RQFAIEKGAPALRAMTFIERAAMLKAVAKHLLSEKERFYALSAQTGATRADSWVDIEG
                     GIGTLFTYASLGSRELPDDTLWPEDELIPLSKEGGFAARHVLTSKSGVAVHINAFNFP
                     CWGMLEKLAPTWLGGMPAIIKPATATAQLTQAMVKSIVDSGLVPEGAISLICGSAGDL
                     LDHLDSQDVVTFTGSAATGQMLRVQPNIVAKSIPFTMEADSLNCCVLGEDVTPDQPEF
                     ALFIREVVREMTTKAGQKCTAIRRIIVPQALVNAVSDALVARLQKVVVGDPAQEGVKM
                     GALVNAEQRADVQEKVNILLAAGCEIRLGGQADLSAAGAFFPPTLLYCPQPDETPAVH
                     ATEAFGPVATLMPAQNQQHALQLACAGGGSLAGTLVTADPQIARQFIADAARTHGRIQ
                     ILNEESAKESTGHGSPLPQLVHGGPGRAGGGEELGGLRAVKHYMQRTAVQGSPTMLAA
                     ISKQWVRGAKVEEDRIHPFRKYFEELQPGDSLLTPRRTMTEADIVNFACLSGDHFYAH
                     MDKIAAAESIFGERVVHGYFVLSAAAGLFVDAGVGPVIANYGLESLRFIEPVKPGDTI
                     QVRLTCKRKTLKKQRSAEEKPTGVVEWAVEVFNQHQTPVALYSILTLVARQHGDFVD"
     gene            34840..37113
                     /gene="tynA"
                     /locus_tag="ACHOSC_RS01195"
                     /old_locus_tag="pgaptmp_000239"
     CDS             34840..37113
                     /gene="tynA"
                     /locus_tag="ACHOSC_RS01195"
                     /old_locus_tag="pgaptmp_000239"
                     /EC_number="1.4.3.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415904.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005507 - copper ion binding [Evidence
                     IEA];
                     GO_function: GO:0008131 - primary amine oxidase activity
                     [Evidence IEA];
                     GO_function: GO:0048038 - quinone binding [Evidence IEA];
                     GO_process: GO:0009308 - amine metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="primary-amine oxidase"
                     /protein_id="HGU4546253.1"
                     /translation="MGSPSLYSARKTTLALAVALSFAWQAPVFAHGGEAHMVPMDETL
                     KEFGADVQWDDYAQIFTLIKDGAYVKVKPGAQTAIVNGQPLALQVPVVMKDNKAWVSD
                     TFINDVFQSGLDQTFQVEKRPHPLNALTADEIKQAVEIVKASADFKPNTRFTEISLLP
                     PDKEAVWAFALENKPVDQPRKADVIMLDGKHIIEAVVDLQNNKLLSWQPIKDAHGMVL
                     LDDFASVQNIINNSEEFAAAVKKRGITDAKKVITTPLTVGYFDGKDGLKQDARLLKVI
                     SYLDVGDGNYWAHPIENLVAVVDLEQKKIVKIEEGPVVPVPMTARPFDGRDRVAPAVK
                     PMQIIEPEGKNYTITGDMIHWRNWDFHLSMNSRVGPMISTVTYNDNGTKRKVMYEGSL
                     GGMIVPYGDPDIGWYFKAYLDSGDYGMGTLTSPIARGKDAPSNAVLLNETIADYTGVP
                     MEIPRAIAVFERYAGPEYKHQEMGQPNVSTERRELVVRWISTVGNYDYIFDWIFHENG
                     TIGIDAGATGIEAVKGVKAKTMHDETAKDDTRYGTLIDHNIVGTTHQHIYNFRLDLDV
                     DGENNSLVAMDPVVKPNTAGGPRTSTMQVNQYNIGNEQDAAQKFDPGTIRLLSNPNKE
                     NRMGNPVSYQIIPYAGGTHPVAKGAQFAPDEWIYHRLSFMDKQLWVTRYHPGERFPEG
                     KYPNRSTHDTGLGQYSKDNESLDNTDAVVWMTTGTTHVARAEEWPIMPTEWVHTLLKP
                     WNFFDETPTLGALKKDK"
     gene            complement(37171..38670)
                     /gene="feaB"
                     /locus_tag="ACHOSC_RS01200"
                     /old_locus_tag="pgaptmp_000240"
                     /gene_synonym="maoB"
                     /gene_synonym="padA"
     CDS             complement(37171..38670)
                     /gene="feaB"
                     /locus_tag="ACHOSC_RS01200"
                     /old_locus_tag="pgaptmp_000240"
                     /gene_synonym="maoB"
                     /gene_synonym="padA"
                     /EC_number="1.2.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415903.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylacetaldehyde dehydrogenase"
                     /protein_id="HGU4546254.1"
                     /translation="MTEPHVAVLSQVQQFLDRQHGLYIDGRPGPAQSEKRLAIFDPAT
                     GQEIASTADANEADVDNAVMSAWRAFVSRRWAGRLPAERERILLRFADLVEQHSEELA
                     QLETLEQGKSIAISRAFEVGCTLNWMRYTAGLTTKIAGKTLDLSIPLPQGARYQAWTR
                     KEPVGVVAGIVPWNFPLMIGMWKVMPALAAGCSIVIKPSETTPLTMLRVAELASEAGI
                     PDGVFNVVTGSGAVCGAALTSHPHVAKISFTGSTATGKGIARTAADHLTRVTLELGGK
                     NPAIVLKDADPQWVIEGLMTGSFLNQGQVCAASSRIYIEAPLFDTLVSGFEQAVKSLQ
                     VGPGMSPVAQINPLVSRAHCDKVCSFLDDAQAQQAELIRGSNGPAGEGYYVAPTLVVN
                     PDAKLRLTREEVFGPVVNLVRVADGEEALQLANDTEYGLTASVWTQNLSQALEYSDRL
                     QAGTVWVNSHTLIDANLPFGGMKQSGTGRDFGPDWLDGWCETKSVCVRY"
     gene            38906..39811
                     /gene="feaR"
                     /locus_tag="ACHOSC_RS01205"
                     /old_locus_tag="pgaptmp_000241"
     CDS             38906..39811
                     /gene="feaR"
                     /locus_tag="ACHOSC_RS01205"
                     /old_locus_tag="pgaptmp_000241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator FeaR"
                     /protein_id="HGU4546255.1"
                     /translation="MNPAVDNEFQQWLSQINQVCGNFTGHLLTERYTGVLDTHFAKGL
                     KLSTVTTSGVNLSRTWQEVKGSDDAWFYTVFQLSGQAIMEQDERQVQIGAGDITLLDA
                     SRPCSLYWQESSKQISLLLPRTLLEQYFPHQKPVCAERLDADLPMVQLSHRLLQESMN
                     NPALSETESEAALQAMVCLLRPVLHQRESVQPRRERQFQKVVTLIDDNIREEILRPEW
                     IAGETGMSVRSLYRMFADKGLVVAQYIRNRRLDFCADAIRHAADDEKLAGIGFHWGFS
                     DQSHFSTVFKQRFGMTPGEYRRKFR"
     gene            complement(39983..40312)
                     /gene="ydbL"
                     /locus_tag="ACHOSC_RS01210"
                     /old_locus_tag="pgaptmp_000242"
     CDS             complement(39983..40312)
                     /gene="ydbL"
                     /locus_tag="ACHOSC_RS01210"
                     /old_locus_tag="pgaptmp_000242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707676.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdbL family protein"
                     /protein_id="HGU4546256.1"
                     /translation="MMKKTLLFCAFLVGLVSSNVMALTLDEARTQGRVGETFYGYLVA
                     LKTDAETEKLVTDINAERKASYQQLAKQNNVSVDDIAKLAGQKLVARAKPGEYVQGIN
                     GKWVRKF"
     gene            complement(40317..40502)
                     /gene="ynbE"
                     /locus_tag="ACHOSC_RS01215"
                     /old_locus_tag="pgaptmp_000243"
     CDS             complement(40317..40502)
                     /gene="ynbE"
                     /locus_tag="ACHOSC_RS01215"
                     /old_locus_tag="pgaptmp_000243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YnbE family lipoprotein"
                     /protein_id="HGU4546257.1"
                     /translation="MKILLAALTSSFMLAACTPRIEVAAPKEPITINMNVKIEHEIII
                     KADKDVEELLETRSDLF"
     gene            complement(40499..43138)
                     /gene="ydbH"
                     /locus_tag="ACHOSC_RS01220"
                     /old_locus_tag="pgaptmp_000244"
     CDS             complement(40499..43138)
                     /gene="ydbH"
                     /locus_tag="ACHOSC_RS01220"
                     /old_locus_tag="pgaptmp_000244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdbH family protein"
                     /protein_id="HGU4546258.1"
                     /translation="MLGKYKAVLALLLLIILVPLTLLMTLGLWVPTLAGIWLPLGTRI
                     ALDESPRITRKGLIIPDLRYLVGDCQLAHITNASLSHPSRWLLNVGTVELDSACLAKL
                     PQTEQSPAAPKTLAQWQSMLPNTWINIDKLIFSPWQEWQGKLSLALTSDIQQLRYQGE
                     KVKFQGQLKGQQLTVSELDVVAFENQPPVKLVGEFTMPLVPDGLPVSGHATATLNLPQ
                     EPSLVDAELDWQENSGQLIVLARDNGDPLLDLPWQITRQQLTVSDGRWSWPYAGFPLS
                     GRLGVKVDNWQAGLENALISGRLSVLTQGQAGKGNAVLNFGPGKLSMDNSQLPLQLTG
                     EAKQADLILYARLPAQLSGSLTDPTLAFEPGALLRSKGRVIDSLDIDEIRWPLAGVKV
                     TQRGVDGRLQAILQAHENELGDFVLHMDGLANDFLPDAGRWQWRYWGKGSFTPMNATW
                     DVAGKGEWHDSTITLTDLSTGFDQLQYGTMTVEKPRLILDKPVVWVRDAQHPSFSGAL
                     SLDAGQTLFTGGSVLPPSTLKFSVDGRDPTYFLFKGDLHAGEIGPVRVNGRWDGIRLR
                     GNAWWPKQSLTVFQPLVPPDWKMNLRDGELYAQVAFSAAPEQGFRAGGHGVLKGGSAW
                     MPDNQVNGVDFVLPFRFADGAWHLGTRGPVTLRIAEVINLVTAKNITADLQGRYPWTE
                     EEPLLLTDVSVDVLGGNVLMKQLRMPQHDPALLRLNNLSSSELVSAVNPKQFAMSGAF
                     SGALPLWLNSEKWIVKDGWLANSGPMTLRLDKDTADAVVKDNMTAGSAINWLRYMEIS
                     RSSTKINLDNLGLLTMQANITGTSRVDGKSGTVNLNYHHEENIFTLWRSLRFGDNLQA
                     WLEQNARLPGNDCPQGKECEEKQ"
     gene            43346..44335
                     /gene="ldhA"
                     /locus_tag="ACHOSC_RS01225"
                     /old_locus_tag="pgaptmp_000245"
     CDS             43346..44335
                     /gene="ldhA"
                     /locus_tag="ACHOSC_RS01225"
                     /old_locus_tag="pgaptmp_000245"
                     /EC_number="1.1.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-lactate dehydrogenase"
                     /protein_id="HGU4546259.1"
                     /translation="MKLAVYSTKQYDKKYLQQVNESFGFELEFFDFLLTEKTAKTANG
                     CEAVCIFVNDDGSRPVLEELKKHGVKYIALRCAGFNNVDLDAAKELGLKVVRVPAYDP
                     EAVAEHAIGMMMTLNRRIHRAYQRTRDANFSLEGLTGFTMYGKTAGVIGTGKIGVAML
                     RILKGFGMRLLAFDPYPSAAALELGVEYVDLPTLFSESDVISLHCPLTPENYHLLNEA
                     AFDQMKNGVMIVNTSRGALIDSQAAIEALKNQKIGSLGMDVYENERDLFFEDKSNDVI
                     QDDVFRRLSACHNVLFTGHQAFLTAEALTSISQTTLQNLSNLEKGETCPNELV"
     gene            44446..44868
                     /gene="hslJ"
                     /locus_tag="ACHOSC_RS01230"
                     /old_locus_tag="pgaptmp_000246"
     CDS             44446..44868
                     /gene="hslJ"
                     /locus_tag="ACHOSC_RS01230"
                     /old_locus_tag="pgaptmp_000246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heat shock protein HslJ"
                     /protein_id="HGU4546260.1"
                     /translation="MKKLAAFVALSLLMAGCVSNDKIAVTPEQLQHHRFVLESVNGKP
                     VTSDKNPPEISFGEKMMISGSMCNRFSGEGKLSNGELTAKGLAMTRMMCANPQLNELD
                     NTISEMLKEGAQVDLTANQLTLATAKQTLTYKLADLMN"
     gene            complement(44865..45131)
                     /gene="ydbJ"
                     /locus_tag="ACHOSC_RS01235"
                     /old_locus_tag="pgaptmp_000247"
     CDS             complement(44865..45131)
                     /gene="ydbJ"
                     /locus_tag="ACHOSC_RS01235"
                     /old_locus_tag="pgaptmp_000247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF333 domain-containing protein"
                     /protein_id="HGU4546261.1"
                     /translation="MRAAFWVGCAALLLSACSSEPVQQATAAHVAPGLKASMSSSGEA
                     NCAMIGGSLSVARQLDGTAIGMCALPNGKRCSEQSLAAGSCGSY"
     gene            45405..48929
                     /gene="nifJ"
                     /locus_tag="ACHOSC_RS01240"
                     /old_locus_tag="pgaptmp_000248"
     CDS             45405..48929
                     /gene="nifJ"
                     /locus_tag="ACHOSC_RS01240"
                     /old_locus_tag="pgaptmp_000248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016903 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors [Evidence IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate:ferredoxin (flavodoxin) oxidoreductase"
                     /protein_id="HGU4546262.1"
                     /translation="MITIDGNGAVASVAFRTSEVIAIYPITPSSTMAEQADAWAGNGL
                     KNVWGDTPRVVEMQSEAGAIATVHGALQTGALSTSFTSSQGLLLMIPTLYKLAGELTP
                     FVLHVAARTVATHALSIFGDHSDVMAVRQTGCAMLCAANVQEAQDFALISHIATLKSR
                     VPFIHFFDGFRTSHEINKIVPLADDTILDLMPQAEIDAHRARALNPEHPVIRGTSANP
                     DTYFQSREATNPWYDAVYDHVEQAMNDFSAATGRQYQPFEYYGHPQAERVIILMGSAI
                     GTCEEVVDELLTRGEKVGVLKVRLYRPFSAKHLLQTLPGSVRSVAVLDRTKEPGAQAE
                     PLYLDVMTALAEAFNNGKRETLPRVIGGRYGLSSKEFGPDCVLAVFAELNAAKPKARF
                     TVGIYDDVTNLSLPLPENTQPNSAKLEALFYGLGSDGSVSATKNNIKIIGNSTPWYAQ
                     GYFVYDSKKAGGLTVSHLRVSEQPIRSAYLISQADFVGCHQLQFIDKYQMAERLKPGG
                     IFLLNTPYSADEVWSRLPQEVQAVLNQKKARFYVINAAKIARECGLAARINTVMQMAF
                     FHLTQILPGDSALAELQGAIAKSYSSKGQDLVERNWQALALARESVEEVPLQPVNPHS
                     ANRPPVVSDAAPDFVKTVTAAMLAGLGDALPVSALPPDGSWPMGTTRWEKRNIAEEIP
                     TWKEELCTQCNHCVAACPHSAIRAKVVPPEAMENAPASLHSLDVKSRDMRGQKYVLQV
                     APEDCTGCNLCVEVCPAKDRQNPEIKAINMMSRLEHVEEEKINYDFFLNLPEIDRSKL
                     ERIDIRTSQLITPLFEYSGACSGCGETPYIKLLTQLYGDRMLIANATGCSSIYGGNLP
                     STPYTTDANGRGPAWANSLFEDNAEFGLGFRLTVDQHRVRVLRLLDQFADKIPAELLT
                     ALKSDATPEVRREQVAALRQQLNDVAEAHELLRDADALVEKSIWLIGGDGWAYDIGFG
                     GLDHVLSLTENVNILVLDTQCYSNTGGQASKATPLGAVTKFGEHGKRKARKDLGVSMM
                     MYGHVYVAQISLGAQLNQTVKAIQEAEAYPGPSLIIAYSPCEEHGYDLALSHDQMRQL
                     TATGFWPLYRFDPRRADEGKLPLALDSRPPSEALEETLLHEQRFRRLNSQQPEVAEQL
                     WKDAAADLQKRYDFLAQMAGKAEKSNTD"
BASE COUNT        12300 a        13030 c        11739 g        11996 t
ORIGIN      
        1 aaacaacttc acttgtcagc gacaccgctt cgtttttaac atcgcttatg gaaaaaaata
       61 gtctgtttag tcagcgcatc cgtttgcgcc accttcatac attcgtagct gtcgcacaac
      121 aaggaacttt ggggcgcgcg gctgaaaccc ttaatttgag tcaacctgcg ctctctaaga
      181 cattgaatga actggagcag ctgactggcg ctcgcttgtt tgagcgtggt cgtcaggggg
      241 cgcaacttac cttacccggc gaacaatttt taacgcatgc agtcagagtt cttgacgcca
      301 tcaacactgc cggacagtcg cttcatcgta aagaaggtct taataatgat gtcgtcaggg
      361 ttggtgcact acctactgcg gcactgggga tattaccttc ggttataggt cagtttcatc
      421 agcaacaaaa agagacgacc ttgcaagttg cgacaatgag taaccctatg attctggcgg
      481 gtttaaaaac cggggaaatc gatatcggca ttggtcggat gtcagatcct gaactgatga
      541 ccgggcttaa ttacgaactg ctgtttcttg aatcgttgaa gctggttgtc cgccctaatc
      601 acccgctact tcaggagaac gtaacgctaa gccgggtgct ggaatggccg gtcgttgtat
      661 caccagaagg cactgcgcca cgccagcatt cagatgcatt agtgcagagc cagggctgta
      721 aaattccttc gggttgtatc gaaacgctgt ctgcttcgct atctcgtcaa cttacggttg
      781 aatacgatta cgtgtggttt gtcccttctg gcgctgtaaa agacgacctg cgtcatgcca
      841 cgctggtggc cctgcctgtt ccgggacatg gtgcaggcga accgattgga atactgaccc
      901 gcgtagatgc gacgttctct tctggttgcc agttgatgat taacgctatt cgaaaatcaa
      961 tgccgttctg aaaggtgaag ggatctgtcg atccctcctt gaacattttc acaccgtagc
     1021 gaaactaact ggttcacccg ctccgcgagg ttctgccgac acagaatgtt tgtgcagacg
     1081 gaatacatcc accgcctcag ttaatcgtgc cgcctgttct tcaagggaca ccgccgccgc
     1141 tgaggcctct tctaccagag aagcattctg ttgcgtcacc ttatccattt cagaaatcgc
     1201 ctggctaacc tgcgttatgc ctctactttg ttcatccgag gcggcggcga tttcctgcat
     1261 gatatgtgtg acactcgcga cggcatcaac aatagtgctc atggttttcc ctgccgttgc
     1321 cacctcatcc gaccccaggt caattaacct gactgattca ctgatcaagc cttcaatctc
     1381 tttcgccgct tgggcgctgc ggcttgcgag tgtccgtact tcgctggcga caacggcaaa
     1441 tccacggcct tgctcacccg ctcgcgcggc ttcaacggca gcattcagtg ccagaatatt
     1501 cgtctggaaa gcaatactgt tgatgacggc ggtgatctcg gaaattttct tcgaacttgt
     1561 agagatagcg cccatcgttt ttactacacc ggaaaccgtc tgcccgccat cgctggcttt
     1621 aatagaagcc tcttgcgcca gtttgctggc atgatgcgcg ttatccgcat tctgtttcac
     1681 cgtcgcagtg agttgctcca tgctagcggc agtttgttcg atagccgccg cttgttcttc
     1741 ggtgcgagat gacaggtccg cattgccagc tgaaatttcg ctggtgccac gataaatctc
     1801 ttccgcaccc tgtcgaacag tccctactgt catccccagt gaatgctgca tttgctgtaa
     1861 atgacgactt aagcgaccga tttcattacg acccgccggt tcatcattca tcgtcagatc
     1921 gccactggcg attttttcaa tccgttgtgc ggcatgttgc agtggacgaa tgacgatccg
     1981 acgtagcacc ataaatgtta tcagcgtcat gaccagggca agcacaaacg cgccaatcat
     2041 gaacatccca cccaggcggg tgcgctgatg ggccagttcc gccagttggt tggctctggt
     2101 gctacgtatc ttaacggctt tgttcaaaat atcggtataa gcattatcca gcggtcggat
     2161 ctgctcactt tcatgattga taatcgcttc aaacatgcca tttttggcat atttcaacat
     2221 aggttgcata cccgtgatat aagcctgaaa gcgttgattt aattcagtgt cgagggcttc
     2281 atcagcaggt gttttcaccg gtcgattctg ataagcacga taaccttgct gcgactgttt
     2341 aatctccgat tcggcttgcg caatatttcg cttcattgct tccatttccg caatacgact
     2401 tgcagccccg gcctgaatca tattgatccg ggctgaacgc aaaaaatctg aactgttcgc
     2461 taacccggtc cgaatctcaa tctccgcagt aacatcacgc tgatcgcgat cggcctgcca
     2521 gaggaaatag ccagccagag cagaacttaa tgcgaataga actaagatac cgccaagaat
     2581 gcaggcaaat aacggaacca gcctgatgtg atgcaaaaaa cctaatcgat gtgagggagt
     2641 tgtattcatt accgttgtct ctcgtccagg tttactcctg atagaaagtc atcggcactt
     2701 gacggtaatt acttatgacc aaaaacctct ttgcgtcaca tttttacaac aattattgca
     2761 gagaaaatta atgctattgg aatgaagaga gtaacgaatg gctaaactgg ctaacccgaa
     2821 tgacgaaaaa ttctggaaaa cagcacgcaa tttcgtgaag tgattatttt cgtccctaag
     2881 gaaacctcat aggatgcctg cgggtgcctg agactttctg ttttcaggct aaccgggaat
     2941 cagaaagacg aaggccccga ccgatatttc tatcaaccga ggccaacgaa tgaacctagc
     3001 aattcagatc ttagcctctt acccgccatc cggcaaggag aaaggctatg aagcacaacc
     3061 ctctggtggt gtgtctgctc attatctgca ttacgattct gacattcaca ctcctgaccc
     3121 gacaaacgct ctacgaactg cggttccggg acggtgataa ggaggttgct gcgctcatgg
     3181 cctgcacgtc caggtaaggg caagcgcggg gattttcccc gcgcatctga ctagcagatt
     3241 cattttctca tggcacccct tttacacttc agttagtgca tgtttttttc gaggcactaa
     3301 tagaaccatc attacagaca aatttgccgc ctgccgtaca gtgtgagatc ccccctttcg
     3361 aaccagaaca gggtttgcgc ccggcatcgg cataaaagga aacaagcgtt cccataaaca
     3421 aaataagtgc cagttttttc atataacctc catggtttat cgaatcttat gttcgatttg
     3481 aacgttatac gctggtttat tttgataccg ttgatgagat caaagatcgg gtgaattgat
     3541 taaaaataag ccagatggac gtatgggcaa ggattatggt tttatttgtc atacaaataa
     3601 atataatagg cgcttccgat gtagacccgt attcttcgcc tgtaccacgg gtcggtttta
     3661 gtacaggcgt tttctttaaa tatccttcag gaacgtttac gcggcatcat gcgtagaagt
     3721 gtgttgtcct tccagaaata gtggtgtgcc agtgcagccg cagcgtgcaa cccgatgaca
     3781 aaatatccca gattcgccag cgtctcatgc cacgacttta agctatcaac ccgttcgaaa
     3841 ttggcctctg aagcgtaagg catcgtcaaa ccaaacgcaa accacgggtt gccccggtta
     3901 tacatcatca ccaaaccaat cactggcagc gcaataaaca ggagataaat caccaaatgt
     3961 cccaaatgcg ccagtcccgt catcatcggt tttggcttag gtataatcgg cggggttggg
     4021 tatttcagtc ttaacaaaag acgaaccacc atcagcacga gaattgagat accacaggaa
     4081 acatgaacca tgttgatgag tggccgatcg ctacgtggga agaaaccacg aaactccatt
     4141 gcgcaatacg ctgcgataac cagcaaaaag accagccagt gaatgctgat ttgtaacctt
     4201 gagtatttat tttccataac atttcctgct ttaacataat tttccgttaa cataacgggc
     4261 ttttctcaaa atttcattaa atattgttca cccgttttca gtacgttacg tttcactgta
     4321 agaatggaga ttgcccatcc cacaatcctg gtctaagcct ggaaaggatc aattttcatc
     4381 cgaatgttcc tgacaggaga aaaccatgag taaagttggt attaacggtt ttggtcgtat
     4441 cggtcgactg gtgttgcgtc gattacttga agtcaaaagc aacatagacg ttgtcgctat
     4501 taatgatctc acttccccaa aaattctcgc ctacctgctg aaacatgatt caaactacgg
     4561 accattcctc tggagcgttg attttacgga agattcactt atcgttgatg ggaaaagtat
     4621 cgcggtttac gccgaaaaag aggctaaaaa tattccgtgg aaagcgaaag gtgcagaaat
     4681 cattgtcgaa tgtactggct tttatacctc cgccgagaaa tcgcaggcgc atcttgatgc
     4741 tggtgcgaag aaggtgttga tttccgcccc tgccggtgaa atgaaaacta tcgtttataa
     4801 cgtcaatgac gacactctgg atggcaacga caccattgtt tccgtggcgt catgcaccac
     4861 taactgtctt gcgccgatgg ccaaagcctt gcatgacagt ttcgggatag aagtcggcac
     4921 gatgacgacc attcatgcct atactggcac ccagtcactg gtggatggcc cacgtggtaa
     4981 agatttacgt gcttcacgcg cagcggcaga aaatatcatt ccccacacta cgggggcggc
     5041 aaaagccatt ggtctggtga tcccggaact gagcggcaaa ctgaaaggtc atgcgcaacg
     5101 cgtgccggtg aaaacaggtt cggtcactga gctggtgtcc attctcggaa aaaaagtgac
     5161 tgccgaagag gtgaataacg cacttaaaca agcaaccacc aataacgagt catttggtta
     5221 taccgatgaa gaaatagtct cttccgatat cattggcagc catttcggtt cggtgtttga
     5281 tgccacgcaa acggaaatta ccgccgtggg cgatttacaa ctggtgaaaa cggtcgcctg
     5341 gtacgataac gaatatggct tcgtcacgca gcttattcgc accctcgaaa aattcgctaa
     5401 actctgacgc gcacaggcgg aggaaaaaac ctccgcctct tttactcatt acgactgtaa
     5461 ataaaccacc tgggtctgca gatattcatg caggccatgt ttaccatctg cgccgccaat
     5521 accggattta cgccatccgg cgtggaagcc ttgcatagct tcgaagtttt cacggttgat
     5581 gtaagtttca ccaaacttca gccctttaat ggctttcatc gcgacgttca gattttgggt
     5641 atagattgat gaggtcaggc cgtaatcact gtcattagcc attgagatag catcttccag
     5701 cgtgtcaaat gcgaccaccg gcagaaccgg gccaaaggtt tcctcatgca taatcgacat
     5761 ttcctggcga acatccagca gcaatgtcgg cggataataa tatcctttcc cgtctactgc
     5821 tttgccaccc aacgccactc tcgccccttc ttctactgcg cgcgccactt tttgctcgac
     5881 cctttccagc gccgcggcgt taatcaacgg ccccatcgca atgtcgttgc gttcagcggg
     5941 gttaccaaat tgaaccgcct gcatcgcttc acccagccga ttgacgaact gatcataaat
     6001 gcctttctgt acataaacac gttctgcaca gttacacact tgcccactat taatgacgcg
     6061 tgaatcaacg atggctttga ctgccagttc aagatcggca tcgtccatta cgatagctgg
     6121 tgctttacca cccaattcca ggcacacttt ggtgatgttt ttcgccgcag tcgccataat
     6181 cttctcacct gcagagacgc tgcctgtcat actgaccatt gcgacctttg ggttacccgc
     6241 cagttcttgc ccaacggttt caccacgccc cagtacaagg ttaaacacgc cgcgcggaag
     6301 gcctatttca tcgacgattt tggcgaatgc aatcgcattg tttggcgtaa attcactggg
     6361 tttaatgacg atggtattac cggtcaaaag agcgggagcc attttgcggg caatgaggaa
     6421 gaacgggaag ttccacggca gaatgccggt agtcacacca agcgcacgtt taaacaaaag
     6481 aatattttct cctggacgat cgctttgaat aatctcgccc tcgtaacgcc gtgcccactc
     6541 cgccatgtaa tcgatatagt cggcagtaaa agccacttcg acttcagcca gctgctggat
     6601 cttgccccct tcttcaacaa tcagcgcact gatttcactg gcgcgttcgc ggatgccggc
     6661 ggagattttg cgcaaccaac tggcgcgttc aatagcaggc aacgcttccc attctggttg
     6721 tgcacgttct gctgcatcga ttgccttacg ggcatcctcg gcctgaccat cgggtatgcg
     6781 ggaaatgaca gcctctgcag cagggtttac cacatcaatc catgcgtctc cacgccaggt
     6841 aacaaactgt ccatcgatat acataggatg ttgaacgggt actgacatgg gcgactcctg
     6901 tgatttatat gtttgttttc ggtgaatgca ttgttaatta acatcaatca tcagagagac
     6961 ggcatcgtga aaggacaatt ctgtgaaggg cttcataaaa acaagtgtaa aaggaataaa
     7021 aggcagaaat aaaagtcagg agtcatgccc gtttgcaaac aagtggttca caaacgggca
     7081 atttttaacg taacgagcga ctttccatcg cctcgatgag cactgcatca tgtttcagcg
     7141 tttgccatgc atggatgact tctgccggaa aatcaacgtg aacgataaaa tctcgcccgc
     7201 ggggaccgta gccatcgcta tcatcgcgta aacgcggcag ctcgccagtg agtagtgaga
     7261 gataacgctc aaccggccat aatccttgta actgattaat aaagattaca ctgtctgcgt
     7321 tatttcctaa ctgaggaacg aatccgggat aacttaacca gcgtggtgca tcgggattgt
     7381 ccccagtcat acggcggaac gtcgccatcg tgcgccgctg catggtgggg tcctgaacaa
     7441 cgatagccgt atgaactcgt tctacggcct gattcaatag cgcgatgcta aagcgtgcgt
     7501 tttcaccgca gtttgttgac tggtcttcaa tccagatttt ttcatgcgga atgtgccaga
     7561 actgatgagc gatatccgcc aggatggtcg cttctgctct gccagtggtg cggatagtgt
     7621 tgtagtgcgg atgctgtgcg atggcgctat acaaaaaagt tgtcgagtga ccgataccac
     7681 cactaatcag taaaggaatt tgctgatcgc gagcaatctt acatgccgcg tcgatagtcg
     7741 gcataaccgc attgcctgca aggatcacgc aatcggcctg atacggcacc tcaccggaga
     7801 aatcatcctg cgccagccac tgtccgataa catttatggc atctatagtt gccggcgaaa
     7861 gcgtcggaaa cggggtaatg ttcatggttc ctccttatcg ttcccttaag aatactgcgt
     7921 gagataatag tgagaagcgg agcactctga aaggaggaag tcgtatgtcc ttaaccgcca
     7981 ctccgcaagc ctgacatatc ttttaaggaa atgctgtaga taaacattat tcatcttgca
     8041 gcagagaatt gtatttttct gaattcctga atttttaccc ttaccgtttt agaattaata
     8101 aaaaacccgg cgctaaatgc ttaccgggtt ttttctttat caggcaaata gcagggttaa
     8161 ccgctaatct gctccatcgc ctgcaaaata cgcttatctg aaatcggata aggcgtacca
     8221 agttgttgag cgaagtaact aacgcgcaac tcttctatca tccaacggat ctctttcacg
     8281 tcttcatcct cacgacgtgc gggcggcagt ttgttgatcc attgctgcca cgcctgctgg
     8341 acgttttcga ctttcagcat ctgagcacgg tcgcgatgtg gatcaaccgc cagtttttcc
     8401 agccgttttt caatcgcctg caaatatcgc agcgtgtcgc ccagccgttt gaagccgtta
     8461 ccagtgacaa aaccgcgata taccaacccg cccatctgcg ctttaatgtc agaaagcccc
     8521 agtgccatgg tcatatccac ccgccctttc agacgtttgt tgatattgaa caccgcagtg
     8581 aggatttgct cgacctgctt cgcaatatcc accaccgtgt cattcagttc ggcgcgcact
     8641 ttttcatgca gcgcagcaaa gccttcttcc gtccagaccg ggccaccatt ggcgtcgatc
     8701 aattgatcca caccgcagga gatacagtcg tcgatcagct ccagcacttt gccatacggg
     8761 ttaaagtaca gtcccagctt ggctttgttc ggtaactttt catgtaaata tttgattggc
     8821 gatggaatat tcagcagcag tagacggcga agaccgttcc acattgcctg cttttgctcc
     8881 agcgggttat caaacagttt gatcgccaca ctgtcgcgct catccaccag cgccggccac
     8941 gccttcactt tgtagttgcc acgcttctgt tcgtagcttt ccggcagctg accaaaactc
     9001 cagatatgta agccgctctg ctcgataccg tcatccgcca ccgcagatag cgtttcctgc
     9061 actttgcctt tcagcgcatc tttcagatct tgtagcgagc gcccttcttt tagcttcttg
     9121 tttttgtcat ccaccacgcg gaaggtaatt ttcaggtgat caggcacctg atcccagtgc
     9181 cagtcttcgc ggtcaacggt aacgccggtc atccgccgta actcgcgctc aaggctgtcg
     9241 agcaacggta actccagcgg tttgacgcgg cctaaaaacg cttcggcata gtttggcgcg
     9301 ggtacaaaat tacggcgtac cggtttcggc aacgatttaa tcagagcaat caccagttcg
     9361 cggcgcagac cggggatctg ccattcaaac ccgctttcct caacctggtt aagtaacggc
     9421 agcggaatat gtacggtcac accgtcagca tccgcgccgg gctcaaactg atagctcaaa
     9481 cgcagcttga gattgccctg atgccagaag ttcgggtaat ccagcttgct gattttttct
     9541 gcgccctctt tgatcaacat gcttttttca aagttgagca aatcaggggt ttcgcggctg
     9601 acttttttcc accagctgtc gaagtgacga gcggagatta catcgtggct gatgcgctgg
     9661 tcgtagaact caaacaacgt ttcgtcatca accagaatat cgcggcgacg tgatttgtgt
     9721 tccagctctt ccacttcggc ccgcagtttc aggttttcac ggaagaatgc gtgacgcgtc
     9781 tgccagtcac cttccaccag cgcatggcga ataaagagtt cacggcataa cgccggatcg
     9841 atctggctgt agttgacctt gcgcgcggca acaatcggca aaccataaac agtgactttt
     9901 tccgttgcca tcaccgcgcc ctgcgcccgt tcccagtgag gttcgctgta ggtgcgttta
     9961 atcaaatgct gagcgacagg ctccacccat tccgggtcga tacgcgcagc aatgcgcccc
    10021 cacaggcggc tggtttctac cagttccgcc accattaccc atttcggcgg ttttttgaat
    10081 aaaccagaac cgggaaagat ggagaaacgc gcgttacgtg cgccggtata ttcttgttta
    10141 tcggcatctt tcatgccgat atgggaaagt aaacccgtca gcaacgcaat gtgaatttcg
    10201 cgatactccg ccggttcgct gttaaccgga atgccaagtt ctttcaccac ctggcgcaac
    10261 tgggtgtaga tatcctgcca ttcgcgcacg cgcagatagt tgagataatc gatacgacac
    10321 aggcgacgga aggcgttgga agaaagcgcc ttttgctgct cgccaagata attccacaga
    10381 ttcacaaacg cgaggaagtc agactctttg tcgtggaagc gacgatgttt ttcgtccgat
    10441 gcctgctgtt tatccatcgg acgctcgcgc ggatcctgaa tggaaagcgc ggacgtgata
    10501 atcatcgcct cacgcacgca gccatgtttt tgcgcttcca gcaccatacg cgccagacgt
    10561 ggatcgacag gcaactgcga gagctggcga ccgagcggcg tcagtttata ggcgctggcc
    10621 tgttcatccg tggtgatcgc gcccagttct tcgagcagac gcacgccatc ctggatattg
    10681 cgtttatccg gtgcttcgac aaacgggaac gcagcgatat cgcccagccc cagcgcggtc
    10741 atcagcaaaa taaccgaggc caggttggta cgcagaatct ccggatcggt aaactccggg
    10801 cgcgaaagga aatcgtcttc ggaatagaga cgaatacaga tcccttcgga cacacgccca
    10861 cagcggcctt tacgctgatt ggcagacgcc tgcgaaatgg gttcaatcgg caaacgctgc
    10921 actttggtgc gatagctgta gcggctgata cgtgctgtac cggggtcgat aacgtattta
    10981 atccccggta cggtcagcga ggtttccgcg acgttggtcg ccagcacaat gcgccgtccg
    11041 ctgtgcgact ggaacacgcg gttctgttcg ctgttcgaaa gccgcgcata aagcggcaag
    11101 atttcggtat gacgcagatt tagcttgttc agcgcatcgg cggtatcgcg aatttcccgc
    11161 tcaccactca taaagatcag aatgtcgcca gggctttcct gactcagttc gtctacggcg
    11221 tcaaaaatcg cctgcaactg atcgcgctcg gtgtcgtcgg cttcttcaac aatcggtcga
    11281 tagcgcactt ccaccggata ggtccgaccg gagacttcga taatcggcgc attattaaag
    11341 tggcgcgaaa agcgttccgg gtcgatagtc gcggaagtga taatgatttt taggtcagga
    11401 cgccgcggca gcaactcttt caaatagccg agcagaaaat cgatattcag gctgcgttcg
    11461 tgtgcttcgt caataatgat ggtgtcgtac tgcatcagca ggcggtcttg ctggatctcc
    11521 gccagcagga taccgtcggt catcagcttg accatcgtgt tatcactaac gtgatcgctg
    11581 aaacgcactt tgtaaccgat gcaaccgccc ggctccgttt tcagctcttc cgcaatacgg
    11641 ttcgccaccg ttcttgccgc caggcgacgc ggctgggtat ggccgatcag ccctttaatc
    11701 ccgcgcccca gctccataca gattttcggt aactgagtcg ttttaccaga acccgtttcc
    11761 ccggcgacga tcaccacctg gtgatcacga atcgcttcga gaatgtcctg ttttttctga
    11821 ctaaccggta aattgtcagg ataagtaatt tccggtcgtg ccgcttcacg cagcaggact
    11881 ttccctgccg cctggtcaat ctctttcgcc atctcctgga aaatggcctg ttgtgcatca
    11941 ggatttttaa ccttcttaac accgtgcaga cgccgagaaa aacgcagtct gtcgcgcagc
    12001 atcagcgaat ccagccgctg ctgcaaggcc gtaaaggtca atttttgttg ttctgtcata
    12061 acgttagtgg gcagtagtct gcctgattaa ttttctttta aatgatagat atagagtacc
    12121 acatcgcggc tttctatgcg ttgttcaata aattcgaaca taggcttcga tttattgcgc
    12181 tatctctgtg gcaggattgt gaataaagtg tcagcaagca acggggcatc gcccattcaa
    12241 acatctataa ggaaacacca tgagcaaggt attagttctt aaatccagca tcctggcagg
    12301 gtactctcag tctaatcagt tgtccgatta ttttgttgaa caatggcgcg aaaagcactc
    12361 cgctgatgaa atcaccgttc gcgacctggc tgcaaatccg attccggtac tggatggcga
    12421 actggttggt gcactgcgtc cgagcgatgc gccgctgact ccgcgtcagc aggaagctct
    12481 ggcgctttcc gatgagctga ttgccgagct gaaagcccac gacgttatcg ttattgcggt
    12541 accgatgtat aacttcaaca tctcaactca gttgaaaaac tattttgacc tggttgcacg
    12601 cgcaggcgtt actttccgct ataccgagaa cggtccggaa ggtctggtaa cgggtaaaaa
    12661 agccatcgtt attaccagcc gcggtgggat ccacaaagat ggaccaacgg acctggtgac
    12721 gccgtatctg tccacgttcc tcggctttat cggcattacc gatgtgaaat ttgtcttcgc
    12781 cgaagggatc gcatacggtc cggaaatggc agcgaaagcg cagtctgacg cgaaagcggc
    12841 catcgacagc attgttgctg cataaccgga cacgatcttg atgtggactg tagttcagtc
    12901 cacatctcaa tccacttacc tgttttccca taatttcagc atcgctttgt gatcttcctt
    12961 aagcacaata tgcgaacgtc tggctcgaat ataactaatc gcttcatcaa cggttttaca
    13021 gtgtccgtaa cataacaacc atgccgccac taccagcgca ctgcgcgata atcccaacgc
    13081 gcaatggacc agaacgctgc cttgctcttc gcgtaatgtt tccagcatcg ccacggcctg
    13141 tcggagctcc ccctcttccg gaaccaccag atccagcatc ggtacacaga aatagagtcg
    13201 atcttttgtc gctcgtcccc gagggaattc aaaggtgacg tccagaaccg cattctgtgc
    13261 cggaatatgt cgtggaaacg cccctaaata aacaccagca gtaattttgc tcaccggctc
    13321 caggcgacga caaaaccaac gcatagacag ccacatcccg atgcgccagg gcaatgtcag
    13381 ccagtaaacg gcgggcggga gtttcccctg actatctttc cctgttgtta tcgcgccaag
    13441 cccaccgtac ccacggccaa taatgagtag cgataatact ggccaacata accagactga
    13501 ccaccataac tgaatcatca ccattagctc catcaacaca atgcacgagc ccgcgcctac
    13561 gacatatggc agtgctattt tgattcgacg ttgatcaggt ttctgataat tccaacgacg
    13621 atcgacgggc accatccagt caatcaacat acctaccgcc agccctgtga tgacatcaat
    13681 aaaatgatgc tgccaggtcg tcagagtaga aatagcgatg agtaaaaacc atccgccaca
    13741 gactttacgc cacctctcag ccagatgctg acgaaagtga cgccaaagta gccagcagag
    13801 aataatatgc agcgaaggag actggttata aggcagatca aacagttcaa gttgcgaaaa
    13861 tagccatccc gtcaccccac tcacttcagg acggataaaa ctaaacttca gcggatagag
    13921 caaaaaaccg cagcaggcca ttaccgttgc cagaataagc cggtggacaa gtcggcgctg
    13981 ttcgaatgtc gtgctacaaa cgaacagcga aaatccatat aaaagatcca gactccagta
    14041 aggaacaata gtccagggaa gaaaagggat cgccgtttcc caaccgaata cctgactggg
    14101 gatatcatgg ctgttaaggt cctgaaccgc ggtgaactga ttaagagatc cataggtgaa
    14161 gaagaaaaac ggggccagca acaataacca gccagcgcct tgtagcaata cgttccgacg
    14221 ttcagttatc acgacgcacc gccatcgaaa ccgtaaaaat accccactca tcaatccgtt
    14281 gtgtgcattt atcaaatccg gcatcacgca cgagtgaatc catctcccct tgcgaacgca
    14341 cgcgcattac ccacggttta ccatctttat gactggttaa caccccggca atcatctcca
    14401 gttgagggtg ccacggctgc ccggtgtaga tgagaatgcc gcccggttcg atggcattgg
    14461 caagacctgc gagtgagttt tttacctgct cattttcggg aaaaagctca tacaggccag
    14521 agacaatcgc cagcgtaggc cgcggagtta acgcgctgag ttcctccggg ttaaacgcat
    14581 cgccctgttc aaaacgcacc cgcccagaca ttccccgttg ggcaatcatc gcttgcccct
    14641 gtgcaacatt taactcactg taatcacgta acaaaatatc gcttacggca ggctcgtttg
    14701 ccagcgcatc cagtacatag cgcccatgcc ctgcggcaat gtcaaccacg cggacggcta
    14761 aacctttggc gtggagatcg gcaacggctt gtttaatcag tatttgtaaa tgggttttac
    14821 gctggcgaat accgcgccag ccaacactgt tcaggtagat tttgtcaatt aagcgcccga
    14881 atgcgttact gccttgcggt tgattttgat agacataatc cagggaactg cctgaatcaa
    14941 agccggtgct cattccgaga tgcaggcccg cagagtgcgc cccgaacaat ttcatcgcct
    15001 ttcgcataaa gcgatacgcc aaatcaaccg gcgataatgg cacgggtcca cctgaaagga
    15061 gccgccagcg atccgctgat ggtccagtgc ggtcttcatg ctgataatca aatttttgtg
    15121 acttgttagc atataaacga ctaataaagc tttgcatttt ttcaaatgcc tgcgccctgt
    15181 tctcttcacc caacgtgtcg tgataaaagc ctggcagcag atgcagctct ttcagagggc
    15241 tacgtaatct ctgataaaaa tcaatctgcg gttggcgatg caccacatag tcatcgcctg
    15301 atatcagaag ttgcgtgggg agcgtaatcg ccgccgcatc tctaataata cgttcagacg
    15361 ttttgtagag atcgagcaag atattaacgg caatcgcccg tgtgatcagc ggatcattat
    15421 tgaaactcgc cacccgttgc cgatcgtggg tcaaatagcg tcctttcaca taggaattaa
    15481 taaaaaacag accacgcaga cgatgccata acgccagcgc aggacgtgcc agcggtacat
    15541 acaatttaac cttaaaggcc ggagaagcca gcaccagccc gcgaattgca ggcgcataat
    15601 catgaaccca tgtggcaacc agcactgcgc cgacgctttg cgcgatcact accacctcat
    15661 ccagtccgac ctggctatcg ctggcagcaa aacgaacaaa ttcatcgaca tcctgcactg
    15721 aacgcgcaag agatgggcta taaccacgcg gccccgaagt ttgtccatgc cctcgggcat
    15781 cccaggcata aaaaacagta tctggcatcg ccagttcatc aacgagatgt tgtagacgac
    15841 cagaatgttc atgaccgcga tgaaataaga cgatgacctt tttagccccg ggctgtaaag
    15901 cgggccagtg ccgataaaac aacgctgtat tatcactgct ggtaaaaaaa tgttccccat
    15961 ggatgcgtga attttccatt ttctctattt tccttcagta acagcagtag cgaataaaat
    16021 aaaaaaatac cggagcggtg aaaattaatg agtcaatcct gtcaaggagt ccgccatgtc
    16081 ctggtaatag tttcccacta tctttaacac caatatctcg tttgatggct gacatcacga
    16141 catcgccgca aaaaccacta ataccaatta acaaacccgc taataatgcc tgtaatgtat
    16201 ttagcggtgt cagcaacggt ccaataatca gtgacgcaat catggtggtg attacgcccc
    16261 ccaccagacc ttccagcgtt tttcccgggc tgactttggg gaccactttt cttctgccgc
    16321 aggatttccc ccataaatac tgtgcaatat cgtttgactc ggttaatgcc aaaagaaata
    16381 acaccagtaa cgccccgccc tggatatttg tggttggcaa cattaataac caggcggcat
    16441 gactcaaggc gaaaacggtc attatccagc cccaaaaaat ggcgctcacg gtatgcagaa
    16501 aaccggaggg gtcccccata aaaacttgcc cggtggctaa tattagaaac ccagccaaag
    16561 gtataaataa tagatacagc tcaaagcaat taaaaccgat aaacaaatag ttaagaggaa
    16621 taacccaata taaccaccgc ggaaaatgta cagatataag cgtacaatat tctttcaatg
    16681 ccagaaaact tatcagcgca aaaaacgtca acgtcatcca gcgtggacct gaaatcacca
    16741 gtgaaaaaca aatgacgatg ccccaccagg tacgaatacg taatgtaact tcatcctctt
    16801 ttctctccgg taaccgccat agcagaaaat ggtttatcag cgttgctaaa attaacaacg
    16861 cgaaaagtgt tgccagagat ttttccagca ttatctttca gcgctcataa gaagcacact
    16921 ccgacaacga ttaatcgcag tccagagaag cagaattgaa gcaatgctcc ataaaagatt
    16981 attccactgc atccactgtg gataaatggc aacggccaga ccccacagac caaatattaa
    17041 cgcgcgatcg cttttgccaa atggaccggc ataactgcga acaccgttaa tcgtctgcgc
    17101 gagtaaaccg caaaactcgg tcaatatggt gcaaaataac atgagtatca cgagtgatgc
    17161 gttactttcc ggtaaaaata aaaatggtaa atagagcgca atatcggaaa taacatcacc
    17221 agtctcattc aaaattgccc caagccgtgt ttgctgattg cactcacgcg ccaacatgcc
    17281 atccagcgca ttgagcgcca tacggataaa aagcacgatg ggcaatagca taaagaggat
    17341 gggttgtgcc accaacatca acagcaatcc ggtaagaaga gaaagcgcca gtgcagcaag
    17401 agtgatgtga ttcgctgtaa cgtggtgctt gtaaagccaa aacatcgtcg gccttaacag
    17461 cgactgaaag agcggtttta tctggtagag cgtcattatc aatccttgat aattcggttt
    17521 gcgctatggt gcatcaattc ccctgacgga gatatcgcaa cacgccgctt cattaaaaat
    17581 tcagaatata tcaaggtatt ggggactcac tgtcaggaat aattatcccc aattaatcaa
    17641 agggttaaga aatattacgt tgtggatcaa tacaactcat caagttttct catcgcttgt
    17701 tcccgtattg cgcgcgtctt accgttaagc gccagctggc ggactaaatc cagttcaggg
    17761 ttaacaagct ctaacgccag caaaggatca cgtttcagcc tcgcctttaa tacatcaact
    17821 ggaaaacggg gattattcat cgccacaatc caccactcgg gatcaatttc tgccgccagt
    17881 actgcaggcg gtgtgtggcg gttgttaagt gcgccgaact tgaaaaatgt gagattgctt
    17941 ttcgccagtg tactccacca tatacgatcc ggttgttttg ccagtacatc cagttgtgct
    18001 aacgcatcgg ggtcgagtgc ataatagacg cgtaatgctt catcaacctg cgcacgggag
    18061 ctgttttcct gcctcagaaa aaacttcgcc caaatatcat tgcgtggcgt attttgctgt
    18121 aattccttga ggacaacagc gggtaaatta tcccaactaa ctaatgcact ctctaaaaag
    18181 tcccagtctt ccaaacgtat cagggcggtg gcatttaata gcatctgatc atcaacttca
    18241 ccgtggctga taagctcaga caaaatcata tcagcgctgg tttcatacgc agctgaatct
    18301 gctttcggat tatttttccg atgcgataaa gcacgttgcc aaattttttg tttcatcgac
    18361 gtgctcagat actgattttt cgccagatta ctccacccct caggatcgtt gaaacgtgtc
    18421 aataaccgtt gtagagcagc gtcagagtga attacctgca tatcccgaca gcaaaagtag
    18481 gcaggggatt ttttctccat ccttttaaca aaattaaact gtaagttatc tggcaatgct
    18541 aaaaataaca cacgtattag atcatcgtca ttctcgaatt tttggtataa ataggctgca
    18601 actttgtcac tctcttgtgg cgtcatccgc aataattgac catggtgttt catctctgcc
    18661 agcttgaacg ctaagcttgt gacaacattg tttgatgaat cgttcgccaa ctgcattgcc
    18721 tgattgtagg tgagttgtcc tccagcaccg acctgacgaa tagaggaatc atcactgcgg
    18781 atatagccat caataatgga ttcaggcaat aaccacgcat ctttataaag ctcctgaatg
    18841 atcatatccc aatctctttt cgccagagtc attacgatat ccagagggag attttcacgt
    18901 agcgcaagat tcaacggacc gacaccatca ggtagacgac tcattcgcgt cattatttcg
    18961 acagagagcg gttcacggtc gagcaacccc agaacaacag gctggtaacc caattgctga
    19021 tcggtcaact ccagaatctc attgtcaggc atatatctcg ccatactttc aatcgtttgt
    19081 tcatcattgc tattaataag ttgtttgcgt aggttagctg aaattccttt aagccttggg
    19141 agagttcgca ttatggcaag atcaaatgtc gcaatatctc gtccttcaaa tgcagcaacg
    19201 ttgccttcat ccagcatttg caattcctgc actaactcag gagaaggctt agggcgaaac
    19261 agcgcttctt ttttcatctg attacagaca ttccctgcca tgcgaatatt gcttcgctta
    19321 aaaatttctt tcagagcatc ttcatcattt attagacccc aataatatgc atcaggattt
    19381 attatcatca gcgctttttt gacattttca gtgtcatttt tgtcactcgc acggaccatg
    19441 aattcactat ttgtcgaaga cgcaacaaca ggtgcaatta tgtcattgat aaatattcca
    19501 cgcgcattaa tactcccctt aatcgaagac ggattatcgt aatatgagtc gataaatacc
    19561 agtccacttg catttatcga accggtaatt aacagtggtg aatccgtaac tatattcgtt
    19621 gccgtcacgt taccgagaat aacccatgga ttagaaagag agaaagtgga aatattacct
    19681 ttgactgtca aattaccaag aacaatcaat ttttgattac tctctatatt accttctacc
    19741 actaaatcgc cacgatgcac tttccagtcc gatgcagata aaccgaaggg gacaagattc
    19801 acgctactgt tgatagcttg ttcaacttgt tgtttatctt cagcggtagc cgcagaaaaa
    19861 aatggttcaa tatcaactgt atttgcaaca catcgaagtt ccgcctgtac tggaagactc
    19921 agcataaaca ggggaagtaa acctatccag ccgcaaatcc tggacttaaa catatgcact
    19981 ccatgccatt aaaaaagaaa caaaagggaa atgcatcttt cccttttgta tattcgttat
    20041 tctcgcgaaa taccatccaa cgtagacaac acttcctcag aaagatgcaa tttttcagca
    20101 gccatattct cccgtaaatg cgcaacagaa gacgtccctg ggatcagcaa aatattcggt
    20161 gaacgttgta acagccacgc cagcgccacc tgcatgggtg ttgcacccag gctcgcagca
    20221 acatccgaca gcgtagagga ttgcagcggt gtaaagcccc cgagcgggaa gaacggcacg
    20281 taggcaatgc catcgtgggc caaagcatca atcattgcat catcagcacg gtgcgcgatg
    20341 ttgtattcgt tttgcacaca gacaatttcg gcaatcttgc gcgcctctgc aacctgcgtc
    20401 ggtgtgacgt tgctcaggcc aatatgtttt accaggcctt gttgttgcat ctctgccagc
    20461 acggtcaggc tggcctcaat cgatccttcc gctgggccat gaccatctcc catcataacg
    20521 cgcaggttaa ccacatccag tacgtccagg ccgagattac gtagattatc gtgcaccgct
    20581 ttttgcaact ctgccggaga aaaggcgggc aaccaggaag cgtcctctcc acgccgcgca
    20641 ccaattttag tgacaattgt caggtcgtca gagtaaggat aaagcgcttc gcggataatc
    20701 tgattggtga cgtgcggacc ataaaagtcg ctggtatcaa tgtgattgac gcccaatgcc
    20761 agcgcctcgc gcagcacagt tatagcgacg tggcgatctc ttgggggacc aaaaacccca
    20821 ggacctgcca gttgcatcgc gccataacca agacggttaa cggattttgt accgagagta
    20881 aatgtattgc tgctcatcgc accctgccct gtaataaaac ggattaagaa aaatcagtat
    20941 agaaacgaaa agagataaat aatgcgaggg cgtaaacaag attactggat gagttgctct
    21001 attgtcctga aaaacacaaa acaaaacgcg taaagcgccg catccggcaa taaaaaaacc
    21061 gcaacctgta tcagcgttgc ggtttaggta tcacatgcta aaaattagaa tgttcagtat
    21121 ctggacgtta ctgtttcggt cgcagattct catcaaatac cagacgtttc ctgttcggtt
    21181 caacttcccg caatggctca acttgatgca acgtctgctt acaacgagtc accagcacct
    21241 gatactcatg cgtaccctgc tttttccatg ccagctcctg ctcactgagt tcacgaatcg
    21301 ctttcgccgg gctgccgaca atcaggtaat tggcaggcat ttctgctttg gctttcacaa
    21361 atgcggatgc gccaacaatg ctgttctcgc caatcaccgc gccgtccatc actaccgcgt
    21421 tcattcccac taatgcattg cggcggataa tgcaaccatg aaggatagcg ctatgaccaa
    21481 tatgtccatc ttcttccacg acagtatcct gctcgggaaa accgtgcata acgcaattat
    21541 cctgaatgtt cgcgccatct ttcaccacga tacgaccaaa atcgccacgc aggctggcat
    21601 ttggcccaac gtaaacgccc ttgcctagaa taacatcgcc gatcaatacc gctgtcggat
    21661 ggacaaaact ctcttctggc acaaccggag tcagaccatc tatctggtaa attggcatat
    21721 cgcctcctgt tcaatattca aaccgccaaa acgttgaaaa tacaggcttc ccggcgcagg
    21781 cagttcaccg accgaggttt cacctttttc actaacgaac gctaaagcag caggcgctac
    21841 gcgctgataa atgttgatac acagctgacg cgccgtatgc cctgcccagt gcgccggaag
    21901 caactcctct ggcaacaatg ggtctttaag gacgacacgg cgataaaaat ggatcagtaa
    21961 aagctgaata tgaaatgcac gctccggggt taactcgtct gctgcctctt ttaacagcgg
    22021 caccagcggg cggaatgact gaataaaggt ttcgtacatg gcattttgtt cagttaaatg
    22081 ccagcactct tccactctgg cacgcagtgc tgcacgagaa agcgccagtg gtatttgcgc
    22141 ttcaaaacaa atcacgttat ccgccacccc cgcttcatgc aaaagtgtct gtacatccgc
    22201 cagtttttgc gacggcgatg ccatcaggct gggtgccagt gcgccaaaac cttgccagat
    22261 caactgcttt ttgacatcag ccagcgttga tttatctaaa ccttccgaga gcaacaggag
    22321 ccatttacca tcccatgcag gttgctctgc gcgataaatt ttactttctg cccgtcgcgt
    22381 caggcgcaag cctttatcac tgaggctata gaaactgcgt cgcccgatgc gggaaacatc
    22441 cagccagcct tctttattaa gacgaaacaa agcggtgcgc acgaaacgct caccaaatcc
    22501 cagcccttcc agcaaagcag ccagactacc cagccagatt tcaccaccac gatgggaaag
    22561 cgaatcacca tacagagagg agatcaaaga tgtgccactg accggaacag cgtttacagc
    22621 atgttggata aaagtaacaa gtttactcat tcgattcagt ctgttgttat tgttgtgatt
    22681 tatgaatcat agcacaggtc gtatcgactt ctggccctgg cgctttacct caccagggcc
    22741 agacgacacg tcaggcacca acaatattgc gcagatcaaa cacccgacac gccttgcctt
    22801 ctgaacgcgg gatactgcca cagttaacga tcatcacatc ggtagagatc cccaccatcg
    22861 acttaatccg atggcgcagc tggtggcaca cctggcaacg ctgctcatgc gtcaatgtca
    22921 ggctgctttc tttcaactcc actttcacag aaagtgaatc aagatgcccc cggcggttca
    22981 cctccagttg gtaatggggc gataaatgtt cgaatttgac aatctcttct tccagttgtg
    23041 acgggaagac atttacaccg cgaatgatca acatgtcatc gctgcgtccg ctgatgcgat
    23101 ccatgcggcg catagtccgc gccgttcctg gtaacagtcg ggttaaatca cgcgtgcggt
    23161 aacgaatgac cggcaatgct tctttggtca gcgtggtgaa taacagttcg ccatgttcac
    23221 catcggcaag cggtgtgccg tcatgaggat taacaatttc aggatagaaa tgatcttccc
    23281 aaatggttgg gccgtcggta gtttccagac actccatcgc cacccccggc cccatcactt
    23341 ctgacaggcc ataaatatcc agtgcggtga tccccagacg ccgttcaatc tctttacgca
    23401 tggcctgtgt ccacggctca gcaccaaaca ctccaacccg cagcgagcaa ccgctggcat
    23461 caccgcccaa ctgccgctcc agctcttcaa tcaggttaag gcaataagat ggcgtaacca
    23521 tgatcatatc tggttgaaaa tcgcggatca gttgcgcttg tttttccgtc tgcccaccag
    23581 acatcgggat caccgtagcg cccagacgtt cggcaccata gtgcgcgccc agcccaccag
    23641 taaacaggcc gtaaccatag gcaacatgaa ttttgtcttt gggcgtgcca cctgccgcac
    23701 gcaaggaacg cgctacaata ttggcccagt tatcaatatc gttttgcgta tagccgacaa
    23761 ctgtcggttt acctgtggtt cccgaagaag catgaatgcg caccacttgt tccatcggca
    23821 cggcaaaggt gtcgaaggga tagttgtcgc gcagatcctg tttggtggta cagggaaatt
    23881 tacgcagatc tgaaagttcc ctgaaatcat caggatgtac gcctgctgcg tcaaatttgc
    23941 gccgatacat cgggacattt tcatacgcgt gtttgagcgt ccatttcaga cgttgtgttt
    24001 gcaacgcctg taactcatca acagacgcgg tttcaatcgg gtcaagcttt gtattggtta
    24061 tcattgtagg gtactcgcag gttgatatgc tcaaacacgc tccagaatca tggcgatgcc
    24121 ctgaccgaca ccgatgcaca tggtgcacaa tgcgtaacga ccgttacgcc gatgcagctc
    24181 atggctggca gccagtgcca ggcgggcacc actcattccc aacggatggc ctaaggcgat
    24241 agcgcctccg ttggggttaa catgtggggc atcatcaggc agccccaatt cgcgtagtac
    24301 acccaacgcc tgggccgcga acgcttcgtt cagttcaatc acgtccatat cgtgaatact
    24361 cagccccgcg cgttccagca cccggcgagt tgcaggcacc ggaccaagcc ccatcaggcg
    24421 cggttccacc ccggcggttg ccatggctac gattcgcgcc cgcggtgtca gtccttgcgc
    24481 tgctgccatc tgttcactgg caataatcaa cgcagcggct ccgtcattca ccccggaggc
    24541 attgcctgcg gtaatcaccc cattggcacg aaatggtgct tttaacccac gtaactgttc
    24601 cagcgtcgtt tccgggcgca gatgctcatc atgttgtatt tctgttacaa cgcctttctt
    24661 gtttttcaac acaaccggaa caatctcctc agccagaatg cctgaggatt gcgcttttgc
    24721 cgtacgttgc tgactgcgta gcgcaaaact atcttgatct tctcgcgata tttttaacag
    24781 ttcagctaca ttctctgccg tttccggcat gctgtcagtt ccaaattgtt gagccatgag
    24841 cgggttcaca aatcgccagc caatagtggt atcaaacatc tcagcctgac gagaaaatgc
    24901 actggttgcc ttgcccataa caaacggtgc ccgagacatt gactccacgc caccagcgat
    24961 cagcaaatcg ccatcgcccg ctttaatcgc ccgtgcggcg aaccccagtg cgtccagccc
    25021 ggaaccacac aagcggttaa tggttgtgcc ggaaacacta tgcggcagcc ccgccagtaa
    25081 cgtcgccatc cgggctacgt tacggttgtc ttctcccgcc tgattagcac agccgaggat
    25141 cacatcatcg atacactccg catcgagacg cgggtttcgc accagcagct cccgcaaagg
    25201 gatagcagcc agatcgtcag cccgaacacc tgataatgcc ccaccatagc ggccaattgg
    25261 cgtacgaatt ccgtcacaga taaaggcttc acgcatcagg cttctcctgt aatggtgccg
    25321 ccgatgcggt gagatttacc gcgaaacagc gcaaccgttt tttgttgttg gttaacaatt
    25381 tcgatgtcgt aaacaccggt ttgcttgccc tgatgacgta cctgcgcagt agcagttaag
    25441 gtgtctccgg caaagcctgg acgcaaaaaa tcaatcgtgc aggcagaagc gacggctgcc
    25501 agcccctggc tattgcaggc gtaggcaaag gcagtatcag ccagtgaaaa tagctgcccg
    25561 ccgtggcaac tttgatgacc gttaagcatt tgtgcagtga cggtcatggt cactacagca
    25621 aagccttcat ccattgagat aatgtcgatg ccaagcgctt tggcgcaggc atcgttctca
    25681 tacattgcat gggcattttg ccaggcctta tgactcataa ccgctctcca gaagcgctcg
    25741 ttggcgcagc aatgaacatg ggcgatagcg ttcttcgcca tagtgatgtt gtagattttc
    25801 caggagcctt aatattcgct gccagccaag ttgcgctccc caggcaagcg ggccatatgg
    25861 ataattcacc ccaagacgca tggcggtatc gatatcctgt tcagaggcca cgcctttttg
    25921 cagcgcatca agggcttcat tgatgatcat tgctaccgtt cgccaaatca gcattcctgg
    25981 gtaatctgca atttgcagca ctgttttgcc ctgctgttgc aggtaataaa tggccttgcg
    26041 ggtcgctgag tccgggttca ccgctgccgc agcaatggtc accaccttgc ccgccatttt
    26101 gtcgaccact accaccgggc gtgccagtcg tattgccagc gcctgtgccg tctcgccttg
    26161 tgtctcaatc aataaaacat cgtcaatttc cgtgacgccg tcacttttct tttctacttt
    26221 cattgggcta aaactgtcgc ttaccgcttc caggccaaca actgcctcac gttccgcgcg
    26281 ccagtcgtac acgcccagcc cacttttctt gcccaaccgt ccaccaatca ccagttcctg
    26341 ttgcaccagc gaaggtaaaa aacgacgctc ctgccagaaa gcgttaaaca ccgaacaggt
    26401 gacagcaaaa ttgacgtcct gaccaatcag atcggttaat tccagcggcc ccatcgggaa
    26461 accagcgcca tcgcgaagtg cagcgtcaat cacttctggt gcagcaacct gctcttccag
    26521 tgcccgccag gcctcggaat aataaggacg cgccacacgg ttaacgataa atccaggggt
    26581 cgaatgacag cgcacaggct gcttacccca actcaacgtt agttcacaca actgctcaac
    26641 aacttccgct gccgttgcca gcccactgac cacctccacc aacttcatca ccggtgccgg
    26701 gttaaaaaaa tgcagccccg caacacgttc aggatttttt acctccgcag caatcgcggt
    26761 tatagagatt gacgaagtgt tagtggtcaa tagcgtttgt ggcgggcaaa cttccgccag
    26821 ctgtgcaaag agcgcttttt tgacttccag acgttcagac gccgcttcaa tgaccaggtc
    26881 cgcagctgcc agcgcgtgaa tatcggtcac cgggatcagg cgtttcaatg tgcgttcaca
    26941 ggtttcagca gtcagttttc cccgcgtcac gcgtgaattt agccgcgcgt gtatcccgtc
    27001 gattgcgcgg gtcagcgctt cagcagaaat gtcatacagt aaaacctgat gtccatgact
    27061 ggcggcaact tcagcaatgc cagcccccat ggtgccgctc ccaatcactg ccacagtttg
    27121 cacatttatc atcatgctat ttccccgtga actgcggtga gcgtttagcc aggaacgcac
    27181 tgacaccttc acgataatct gcgctccgtc cggcaagtcg ctgatagtcg cgttccagat
    27241 ccagttgcgt atcgagcgta ttggtttcag cgctatttat cgcttgcttg ataagtccaa
    27301 gaccaaatgt cggttgtgtc gccagatgcc gtgccagctg ttgcgcggta tctgccagcg
    27361 tttcatcatc aacaacctgc cagatcatcc cccattcgtg cgcctgttca gcactcagtt
    27421 gattccccag cagtgccagc cccatggcgc gcgctcgccc agcaacgcgt ggcagtaacc
    27481 aggttccacc gcaatcgggt attaagccta acttactaaa cgccatgacg aattttgctg
    27541 aacgggcagc aataacgatg tcgcacccca gtgccagtgt tgcgcctgcc cctgccgcca
    27601 cgccattgac tgcacagatc accggttttg gcagttttgc caggcgacgt accagtgggt
    27661 tatagaaacg ttcaactgac atccctaaat ccggtgcggg gcctgtggga tcaacgttac
    27721 gatcgttaag atcctgacca gcacaaaatc cgcgcccggc acctgtaagt aacaggcaac
    27781 ggatagtgtc gtcgcgctcg acctgtttca ggcactctgc cagttgtgcg tgcatctcat
    27841 cattaaaact gttcaggcgt tccgggcggt tgagcgttag tgtcatcacg cccttttcta
    27901 catgactgag gatgaattcc atcattagcg tcctttaaag tcgggcgtgc gtttttgtaa
    27961 gaaagcggag atgccttcat gacgatcttc tgttgccgcc agcaaggtga ataactgtcg
    28021 ctcctgggca agtccggctt gcaaagccac ttcctgcgac tggcgcagcg cttgctttgc
    28081 cgcttgtaag gccaacggcg agtgacgtgc cattttcgat gccagctgta aggcgtattc
    28141 gagggttaaa tcgctgggga agacgtcgct aaccagcccg gcctgctgtg cttgctgggc
    28201 ggtgatactt tctccgctca gcaccatttt gctggctaac gatttaccga cactacggat
    28261 taaacgttgc gttccgcctg cgccaggcat gatgccgaga gtgatttccg gcaacccaaa
    28321 acgcgcgttc tctccggcaa ccaccacatc gcacaacaat gccagttcgc aacccgcccc
    28381 aagcgcgtaa ccattgacgg ctgcgataag tggtttatta aaggcctgca atcgcgccca
    28441 tagctgcgga cgtgtatcgt ttaaggtggc cgcgagatct ttttctgcca tttcgttgag
    28501 atcggcccca gcggcaaaaa agcgtgcatt accggtaatc acacagaccg aaatgctggt
    28561 atcggtagcc gcagcttcca gttcatttac cagttgcatc agcagggcat tatttagcgc
    28621 attacgtgcg gcgggacggt taagggtcag caacaatact cgttgctgac ggctgacgat
    28681 cagttcgctc atgccatccc cttcgcgtca aagtcaacca ccacatcgct ggtcagcggc
    28741 agtgcctggc aactcaacac ataacctgcg gccagttcat ccggttccag actgtaattg
    28801 gtttccatcg ccactttgcc acgcagcact ttgcatttgc aggtcgcaca gacgccgcct
    28861 ttgcaggcat agggcagatc agccccctgg cgcaatgccg catcgagaat gctttcatcg
    28921 tcggcattaa gcacgatttc ccgatcccgc ccatcctgac gtacagtcac tttttgtccg
    28981 tcactttgca cgttaacgct acgtttgacg cgcgtgccag gcgtattaaa ccgctccaga
    29041 tgaatggttt tatctggcat tcccagtgct tttaaggcgg cttccgtctc atccatcatc
    29101 gccgccggac cacaaataaa tgcctcatca taaagacgaa aattaatgag cgaggcccca
    29161 agtgactgta atctttcacc gtcaatacgc ccgtgaagca gatcgctgtc gagggtttcc
    29221 tgactgaaaa tgcacaacaa ctgtaaacgc tgaggatatt tgtctttcag gtctgccagt
    29281 gcctggcgaa acatcatgct ctggctggta cggttaccgt agatcagggt gaactgactt
    29341 tcaggctcgg tttgtaaagt ggtggcgata atcgccagca ttggcgtaat acctgatcct
    29401 gctgcaatag ccagatagcg gccctggcgt tcggcctggg gctgatagcc gaaatgcccc
    29461 tgcggcacca tgacctccag cgtcataccc tggcggatgt gttcgcgggc atagcgggag
    29521 aaacgtccgc cttcaatggc tttcaccgcc acactaattt cgccaggcag gtagctgcgg
    29581 caaatggagt aacaacggcg taattcttca ccatcaagac tggcttttaa ggtcaaatgt
    29641 tgaccggggc gaaagcgata cgcctcctgc aaaggctggg gcaccgcaaa ggtaatggtc
    29701 accgcatcac gggtttccgg ctcgactttt gccactgtta aggaatgaaa cgttgtcatg
    29761 gcatcctcaa atacatttga aataatcgaa aggttcgcgg caactatcgc agcggtacaa
    29821 tgctttgcag gccgtggaac caaattcact gataagtgtg gtatggacgc tggcgcagcg
    29881 cgggcaacgt acttctggcg gcaaatgggc atggcaactg tgtccggcgg gcgggctaat
    29941 gccatactgt cgcagacgct cacgggcatc gggggtcatc cagtcggtgg tccatgccgg
    30001 gtcgagttgc agcacaacct gaacgggggt aaacccgtgg gttgtcattg cctcacgtat
    30061 cgcgccaatc aaatgttccg ttgccggaca accggaataa gtcggcgtaa agccgatcac
    30121 ccatccttct cccatctgtg tcacattacg caccatgcct aaatcggtaa tggtcagcac
    30181 cgggatctcc gggtccggga tctggctgag tagcgcccat atctcatgaa cctgtggcgg
    30241 tgcaatggtg gccagacgtt gcatacccat ctcctctgtt accattgctg accgggcaag
    30301 acacgctgga gatactgcat ttctgccagc atgggtccaa ggtgttcggt atgcagtcct
    30361 tttttaccgc cagtgcgata cgcctgctct tgcggtacgt tcaatgtggc ttcgttgatt
    30421 ccggcaaaaa cttcggcttc ccacgctgcg cgtaaagtgc gtggatcaac cgcaataccc
    30481 tcttcactca gtgcaatatc aatctcgtcg gcatcgaaca gttcggcagt aaaacgccac
    30541 aacttgtcga tggcctgctg catcttttgc cctgatacgt cagtaccatt gcccagtcgc
    30601 tccagccagc cacgactaaa acgcaggtga taacgcgctt ctttaattgc tttggcagaa
    30661 atcgccgcca gttgcggatc acggctttcc atcagacggg taaagagcgc cacatgccat
    30721 gcatcgatga aatactggcg tgcaatggtg tcggcaaaat tgccgtttgg ttgttcaacc
    30781 aacaataagt tgctgaactg gcgctcgtct cgggtgaagg ccagggtatc ttcatcacct
    30841 tctcccgcta attcagcggc atacgataag aagttacgtg cctgacctaa taaatcgagg
    30901 ccaatgtttg ccagtgcgag atcgatttcc agttccggtg cgtgaccgca ccattccccc
    30961 aaccgctggg agagcaccag atagttatcg cccaggcgca aggtgtaagc cgttaactga
    31021 ttcatttccg acctcacatg tgctcaatgc catcagggat ggtgtaaaac gttggatggc
    31081 gatagacctt gctttcagcc ggatcaaaaa attcaccgcg ttcttccggt tgcgaggcaa
    31141 caatttcact cgccttcacc acccaaattg aacatccttc gctacgacgg gtgtaagcat
    31201 cacgggcatt ttccagtgcc atccgctcat cggcagcatg taaactgcca acatggcggt
    31261 gtgataagcc ctgtttgcca cgcacgaaaa cttcgtataa cggccagtaa acattactca
    31321 ttttgaatct ccttatgcga ccttacgggc aagttgtttt tgtgcatggg ccagcgctgc
    31381 ttcccgtacc caggtacctt cttcccaggc tttacgtttg gcgtcgagcc tctcctgatt
    31441 acaaattccg cgaccgttaa ttacttcgtt aaactcctgc cagtcgatct caccaaagcg
    31501 gtagtgaccg ctttcagtgt caaaatgcag atccgggtca ggaacggtca taccgagcat
    31561 ttcaacctgt ggaacggtgt tgtccacgaa acgctggcgg agttcgtcgt tggtgaaacg
    31621 tttgattttc caggcgagac ttctggcgct gtttggcgag ttatcatcgt ttggcccgaa
    31681 catcattaag gctggccacc agaaacggtt aatggcgtct tgcaacatct gcttttgagc
    31741 ttcgctgccc tgcgccagtg ccatgcaggc ttcaaacccc tgacgctggt gaaaactctc
    31801 ttctttgcag attttcacca ttgctctggc atacgggcca taagaagttc ggcataacgc
    31861 cacctgattg acgatcgctg cgccatccac cagccagccg ataacgccga tatcagccca
    31921 actcaatgtc ggataattaa agatggagga atatttcatc cgtccgtcga gcattttttg
    31981 gtagatgtct tctctggcac agcccagtgt ttcggcggcg ctatagagat acagaccatg
    32041 accggcttca tcctgcactt tggctaacag aatggcttta cgccgcaagg ttggtgcgcg
    32101 agtgatccag ttaccttcag gcaacatacc aacaatctcg gagtgcgcat gctgcccaat
    32161 ctggcggatc aatgtctttc ggtaagcatc gggcatccag tcctgcggct cgatagccgt
    32221 ttcctgggct atccgttgct caaagcgttc ttcttgggtc acgtcatcac ctttacgatt
    32281 ccagttttcg tgaattaatt aaaaacacga atcacgttgt tttatgaaag ttacacaatg
    32341 gttaactaaa gtcacaaatt ttgtatactt gaactgtgac agatttcgca ctctattttg
    32401 ataactcatt atttatcagt gcattaatgt ttttagtagt tgatatacag atcacgcagt
    32461 taacatttcg ttaaaagatc ccttgctttt tatgattcgc gatttaacta ttagcaacag
    32521 aaatgtgaaa catctggaga gtagcgatgc agcagttagc cagtttctta tccggtacct
    32581 ggcagtctgg ccggggccgt agccgtttga ttcaccacgc tattagcggc gaggcgttat
    32641 gggaagtgac cagtgaaggt cttgatatgg cggctgcccg ccagtttgcc attgaaaaag
    32701 gtgcccccgc cctccgcgcg atgaccttta tcgaacgtgc ggcgatgctt aaagcggtcg
    32761 ctaaacatct gctgagtgaa aaagagcgtt tctatgctct ttctgcgcaa acaggcgcaa
    32821 cgcgggcaga cagttgggtt gatattgaag gtggcattgg gacgttattt acttacgcca
    32881 gcctcggtag ccgggagctg cctgacgata cgctgtggcc ggaagatgaa ttgatcccct
    32941 tatcgaaaga aggtggattt gccgcgcgcc atgtactgac ctcgaagtca ggcgtggcag
    33001 tgcatattaa cgcctttaac ttcccctgct ggggaatgct ggaaaagctg gcaccaacgt
    33061 ggctgggcgg aatgccagcc atcatcaaac cagctaccgc gacggcccaa ctgactcagg
    33121 cgatggtgaa atcaattgtc gatagtggtc ttgttcccga aggcgcaatt agtctgatct
    33181 gcggtagtgc tggcgacttg ttggatcatc tggacagcca ggatgtggtg actttcacgg
    33241 ggtcagcggc gaccggacag atgctgcgag ttcagccaaa tatcgtcgcc aaatctatcc
    33301 ccttcactat ggaagctgat tccctgaact gctgcgtact gggcgaagat gtcaccccgg
    33361 atcaaccgga gtttgcgctg tttattcgtg aagttgtgcg tgagatgacc acaaaagccg
    33421 ggcaaaaatg tacggcaatc cggcggatta ttgtgccgca ggcattggtt aatgctgtca
    33481 gtgatgctct ggttgcgcga ttacagaaag tcgtggtcgg tgatcctgct caggaaggcg
    33541 tgaaaatggg cgcactggta aatgctgagc agcgtgccga tgtgcaggaa aaagtgaaca
    33601 tattgctggc tgcaggatgc gagattcgcc tcggtggtca ggcggattta tctgctgcgg
    33661 gtgccttctt cccgccaacc ttattgtact gtccgcagcc ggatgaaaca ccggcggtac
    33721 atgcaacaga agcctttggc cctgtcgcaa cgctaatgcc agcacaaaac cagcaacatg
    33781 ctctgcaact ggcttgtgca ggcggcggta gccttgcggg aacgctggtg acggctgatc
    33841 cgcaaattgc gcgtcagttt attgccgacg cggcacgtac gcatgggcga attcagatcc
    33901 tcaatgaaga gtcggcaaaa gaatccaccg ggcatggctc cccactgcca caactggtac
    33961 atggtgggcc tggtcgcgca ggaggcggtg aagaattagg tggtttacga gcggtgaaac
    34021 attacatgca gcgaaccgct gttcagggta gtccgacgat gcttgcagct atcagtaaac
    34081 agtgggtgcg cggtgcgaaa gtcgaagaag atcgtattca tccgttccgc aaatattttg
    34141 aggagctaca accaggcgac agcctgttga ctccccgccg cacaatgaca gaggccgata
    34201 ttgttaactt tgcttgcctc agcggcgatc atttctatgc acatatggat aagattgctg
    34261 ctgccgaatc tattttcggt gagcgggtgg tgcatgggta ttttgtgctt tctgcggctg
    34321 cgggtctgtt tgtcgatgcc ggtgttggtc cggtcattgc taactacggg ctggaaagct
    34381 tgcgttttat cgaacccgta aagccaggcg ataccatcca ggtgcgtctc acctgtaagc
    34441 gcaagacgct gaaaaaacag cgtagcgcag aagaaaaacc aacaggtgtg gtggaatggg
    34501 cggtagaggt attcaatcag catcaaaccc cggtggcgct gtattcaatt ctgacgctgg
    34561 tggccaggca gcacggtgat tttgtcgatt aatcggtgaa tgaagggcaa cggccaatag
    34621 ttgccctttt atttcactaa gttttgtgac gttgtcacat tatgcatgat gtgtacatct
    34681 attttcaggg cacccactgt atgaaaagct ggcacacctg ccaaacaacc tggcaggtgc
    34741 aggcaatccc ctttgcatca gtactgataa tgtgaacctg actaaaccgc ccacagagcg
    34801 cggttgctaa caagaacaca acatctgacg aggttaataa tgggaagccc ctctctgtat
    34861 tctgcccgta aaacaaccct ggcgttggca gtcgccttaa gtttcgcctg gcaagcgccg
    34921 gtatttgccc acggtggtga agcgcatatg gtgccaatgg atgaaacgct taaagaattt
    34981 ggtgccgatg tgcagtggga cgactacgcc cagatcttta ccctgattaa agatggcgcg
    35041 tacgtgaaag tgaagcctgg tgcgcaaaca gcaattgtta atggtcaacc tctggcactg
    35101 caagtgccgg tagtgatgaa agacaataag gcctgggttt ctgacacctt tattaacgat
    35161 gttttccagt ccggtctgga tcaaaccttt caggtagaaa agcgccctca cccacttaat
    35221 gcgctaactg cggacgaaat taaacaggcc gttgaaattg ttaaagcttc cgcggacttc
    35281 aaacccaata cccgttttac tgagatctcc ctgctaccgc cagataaaga agctgtctgg
    35341 gcgtttgcgc tggaaaacaa accggttgac cagccacgca aagccgacgt cattatgctc
    35401 gacggcaaac atatcatcga agcggtggtg gatctgcaaa acaacaaact gctctcctgg
    35461 caacccatta aagacgccca cggtatggtg ttgctggatg atttcgccag tgtgcagaac
    35521 attattaaca acagtgaaga atttgccgct gccgtgaaga aacgcggtat tactgatgcg
    35581 aaaaaagtga ttaccacgcc gctgaccgta ggttatttcg atggtaaaga tggcctgaaa
    35641 caagatgccc ggttgctcaa agtcatcagc tatcttgatg tcggtgatgg caactactgg
    35701 gcacatccca tcgaaaacct ggtggcggtc gttgatttag aacagaaaaa aatcgttaag
    35761 attgaagaag gtccggtagt tccggtgcca atgaccgcac gcccatttga tggccgtgac
    35821 cgcgttgctc cggcagttaa gcctatgcaa atcattgagc ctgaaggtaa aaattacacc
    35881 attactggcg atatgattca ctggcggaac tgggattttc acctcagcat gaactctcgc
    35941 gtcgggccga tgatctccac cgtgacttat aacgacaatg gcaccaaacg caaagtcatg
    36001 tacgaaggtt ctctcggcgg catgattgtg ccttacggtg atcctgatat tggctggtac
    36061 tttaaagcgt atctggactc tggtgactac ggtatgggca cgctaacctc accaattgct
    36121 cgtggtaaag atgccccgtc taacgcagtg ctccttaatg aaaccatcgc cgactacact
    36181 ggcgtgccga tggagatccc tcgcgctatc gcggtatttg aacgttatgc cgggcctgag
    36241 tataagcatc aggaaatggg ccagcccaac gtcagtaccg aacgccggga gttagtggtg
    36301 cgctggatca gtacagtggg taactatgac tacatttttg actggatctt ccatgaaaac
    36361 ggcactattg gcattgatgc cggtgctacg ggcatcgaag cggtgaaagg tgttaaagcg
    36421 aaaaccatgc acgatgagac ggcgaaagat gacacgcgct acggcacgct tatcgatcac
    36481 aatatcgtgg gtactacaca ccaacatatt tataatttcc gcctcgatct ggatgtagat
    36541 ggcgagaata acagcctggt ggcgatggac ccagtggtaa aaccgaatac tgccggtggc
    36601 ccacgcacca gtaccatgca agttaatcag tacaacatcg gcaatgaaca ggatgccgcg
    36661 cagaagtttg atccgggcac gattcgtctg ttgagtaacc cgaacaaaga gaaccgcatg
    36721 ggcaatccag tttcctatca aattattcct tatgcaggtg gtactcaccc ggtagcaaaa
    36781 ggtgcccagt tcgcgccgga cgagtggatc tatcatcgtt taagctttat ggacaagcag
    36841 ctttgggtaa cgcgttatca tcctggcgag cgtttcccgg aaggcaaata tccgaaccgt
    36901 tctactcatg acaccggtct tggacaatac agtaaagata acgagtcgct ggacaacacc
    36961 gacgccgttg tctggatgac caccggcacc acacatgtgg cccgcgccga agagtggccg
    37021 attatgccga ccgaatgggt acatactctg cttaaaccat ggaacttctt tgacgaaacg
    37081 ccaacgctag gggcgctgaa gaaagataag tgattgtttc agacaaaaaa acggcaccag
    37141 gtgccgtttt ttacttatga gcgaaccaga ttaataccgt acacacaccg acttagtttc
    37201 acaccaaccg tccagccagt cggggccaaa atcacggccc gttcctgact gcttcatccc
    37261 accaaacggt aagttagcgt caattaaggt atggctgttt acccacaccg tccctgcctg
    37321 taagcgatcg ctatattcca gagcctggga gagattttgc gtccagacac tggcagttaa
    37381 gccatattcc gtgtcgtttg ccagttgtaa cgcctcttct ccatccgcta ctcgcaccag
    37441 gtttaccacc ggaccaaaca cctcttcacg agttaagcgc aatttagcat cgggatttac
    37501 caccagcgtt ggcgcaacat aatacccctc tccggctggt ccattcgacc cgcgaatcag
    37561 ctctgcttgc tgtgcctgcg catcgtcgag gaatgaacac actttgtcgc agtgcgcacg
    37621 agaaaccaaa gggttaatct gtgcaacagg tgacatcccc ggtcccactt gcaacgattt
    37681 taccgcctgc tcaaatccac taaccagcgt gtcaaacaac ggcgcttcaa tataaattcg
    37741 cgaactggcg gcgcatactt gcccttgatt caggaagctt ccggtcatca agccttcaat
    37801 aacccattgc ggatcagcat cttttaatac aattgccggg tttttaccgc ccagttccag
    37861 cgttacacgc gttaagtgat cagcagcagt tctggcaata ccttttcccg tcgcggttga
    37921 accggtaaaa ctgattttcg caacatgagg atgtgacgtc agggccgcgc cgcatacagc
    37981 acctgacccg gtgacgacat taaaaacgcc atcagggata ccagcctcgc tggccagttc
    38041 cgccacgcgc aacatcgtca gtggcgtggt ttccgaaggc ttaatcacga ttgaacagcc
    38101 tgctgccagt gctggcatca ccttccacat accaatcatc aacggaaagt tccatggcac
    38161 aattcccgcc actacgccaa ccggctcttt acgcgtccag gcctgataac gcgccccctg
    38221 gggtaaggga atcgacaagt ccagcgtttt acccgcgatt ttggtcgtta acccggcggt
    38281 ataacgcatc cagttcagcg tacagcccac ttcaaaagca cgagaaatgg caattgactt
    38341 gccttgctcc agggtttcca gttgcgccag ctcctcactg tgctgctcca ccagatcagc
    38401 aaaacgtagc agaatacgtt cacgctctgc gggtaatcgc ccggcccagc gacgcgagac
    38461 aaaggcccgc caggcagaca tgactgcgtt atctacatcc gcttcgttgg catcagcagt
    38521 agacgcaatt tcttgcccgg tggccggatc aaagatcgcc aatcgttttt cactttgtgc
    38581 ggggcctgga cgaccatcaa tataaagacc gtgttgacga tcgagaaact gttggacctg
    38641 gcttaatact gctacatgcg gctctgtcat cacttttcct tattatttac ccagtgtgat
    38701 ttcagtgtac gcagcgacaa gaaaagtcac ttttttcgct gtgacattcg ctttgtcagc
    38761 tgtgacaagc tccgcaaatc gtgacaataa ccgcttatgt ctctatgcct gagaagactt
    38821 atttcctata gtcaggctca ttagcttcgt ttactgcaac acaaatgcaa caataaaaat
    38881 acatttcaca gagcgaaaac gtgccatgaa ccccgcagtg gataatgagt ttcagcaatg
    38941 gctttcccaa atcaatcagg tatgcggaaa ttttaccgga cacctgctga ctgagcgtta
    39001 cacgggtgta ctggatacgc attttgccaa aggactaaag ctgagtaccg tgacaaccag
    39061 cggggtgaat ttatcccgca cctggcagga agtaaaaggc agcgacgatg cctggtttta
    39121 caccgttttt cagcttagtg gtcaggcaat aatggagcag gatgagcgtc aggtgcagat
    39181 tggcgctggc gatattacgt tactcgatgc ctcacgcccc tgttcgcttt actggcagga
    39241 gtcttctaaa cagatttcat tacttttgcc acgcactctg ctggaacaat attttcccca
    39301 tcaaaaacct gtctgcgcag aaagactgga cgctgactta cccatggtgc aactcagtca
    39361 tcgcctgtta caggagagca tgaataatcc ggcactttct gaaacagaaa gtgaagctgc
    39421 gctacaggcg atggtgtgtc tgctgcgccc ggtacttcat cagcgggaat ctgttcaacc
    39481 tcgtcgtgaa cgtcagtttc aaaaagtggt tacgttgata gacgataata ttcgcgaaga
    39541 gatattacgc ccggagtgga tagccggaga gacaggtatg tcagtacgta gtttgtaccg
    39601 aatgtttgcc gataaaggtt tggtagtcgc gcaatatatt cgtaaccgtc gtctcgattt
    39661 ttgtgcagat gcgattcgcc atgccgcaga tgatgaaaaa ctggcaggca tcggctttca
    39721 ttggggattt tctgaccaga gtcatttttc aacggtattt aagcaacgct ttgggatgac
    39781 gccaggcgag tatcgacgta aattccgcta actaattggc gttgtgtgta ctgcaacgcc
    39841 aattagttaa aacagatatt tgactatgca tttttcaggg cgaaaggcaa tttgacagga
    39901 gtttttgaat attcaccttg tttcaggaaa atagatcaat cgctttctcc tgtagcgtta
    39961 tcccgatatt gcggttttgc tattaaaatt ttcgcaccca tttaccgttt atcccttgta
    40021 catattcccc cggtttggcc cgagctacga gcttttgccc ggcgagtttc gcgatatcat
    40081 ctacagacac attattttgc tttgccagtt gttggtaact cgctttacgt tcggcattaa
    40141 tgtcggttac taatttctct gtttcagcat ccgttttcag cgcaaccaga taaccataaa
    40201 atgtttcacc tacccgcccc tgagttctgg cttcatccag agtcaatgcc attacattgc
    40261 tgcttaccag cccaacaaga aacgcacaaa aaagtaatgt tttcttcatc atcgcctcag
    40321 aaaagatcgc tacgagtttc aagcagctct tcgacatctt tgtctgcctt gatgatgatc
    40381 tcatgctcaa ttttaacgtt catattgata gtgatcggtt ccttgggcgc agcgacttca
    40441 atacgcggcg tacaggcagc cagcataaaa gatgacgtca acgcagccag taaaattttc
    40501 attgtttttc ctcacactct tttccttgcg gacagtcatt tcccggcaga cgtgcgttct
    40561 gctccaacca tgcctggaga ttatcgccaa agcgtaaact gcgccacagc gtaaaaatat
    40621 tctcttcatg atggtaatta aggtttaccg taccgctttt accatcaacg cgactggtac
    40681 ctgtaatgtt ggcctgcatg gttaataaac cgagattatc taagttaatt tttgtcgatg
    40741 accggctaat ttccatatag cgcaaccagt taattgctga acccgcagtc atattgtctt
    40801 tcaccaccgc atctgcggtg tctttatcca gccgcaaggt cattggcccg ctatttgcca
    40861 gccaaccatc tttcactatc catttttcgc tgtttagcca taacggcaat gcaccactaa
    40921 atgccccgga catggcgaat tgtttcggat tgacggcgct aaccagttcg ctggatgaga
    40981 gattattcag ccgcaacagc gccgggtcat gttgcggcat acgtaattgt ttcatcagta
    41041 cgttaccgcc taacacatcg acgctaacat cagtcaacag caagggttct tcttcagtcc
    41101 acggataacg cccttgcaaa tcagccgtaa tatttttcgc tgtcaccaga ttaatcactt
    41161 cggcaattcg caacgtaacg gggccgcgag tccccagatg ccaggctcca tcggcaaaac
    41221 ggaaaggcag gacaaaatcg acaccgttaa cctgattatc tggcatccag gcactaccgc
    41281 ctttcaacac gccgtgtcct cccgcgcgga atccttgttc aggcgcagca gaaaatgcaa
    41341 cctgagcata cagttcacca tcgcgtaagt tcatcttcca gtcgggtggc accagcggct
    41401 ggaatacagt cagtgattgt tttggccacc aggcgttacc gcgcagacga ataccgtccc
    41461 agcgaccatt tacccgaacc gggccaatct caccagcatg taaatcgcct ttaaagagga
    41521 aatacgtcgg atcgcgccca tcgacgctaa attttaaggt tgatggcggt aacacactgc
    41581 cgccagtgaa cagcgtttgc ccggcgtcca gtgacagcgc gccgctgaag gagggatgct
    41641 gtgcgtcacg tacccagacg acgggcttgt cgagaattaa tcgcggcttt tctaccgtca
    41701 tcgtaccgta ttgtaactgg tcgaaaccgg tggagaggtc ggtcagcgta atcgtgctgt
    41761 catgccattc accttttcct gcgacatccc aggtggcatt catcggtgta aaactgcctt
    41821 ttccccagta gcgccactgc cagcggcccg cgtcagggag aaaatcattc gccagcccat
    41881 ccatatgcag cacgaaatcg cccagttcat tttcatgcgc ctgcaaaatg gcctgcaaac
    41941 gtccgtcaac accacgttgg gtgactttta cacccgctaa aggccagcgg atttcatcga
    42001 tgtccagcga atcgatgact cttcccttcg aacgtaataa cgcgcctggc tcaaaggcca
    42061 gcgttgggtc agtcagactt ccacttagct gcgcgggtaa gcgggcatat aaaatgaggt
    42121 ccgcctgttt cgcttcaccg gtcagttgca gaggcagctg gctgttatcc atgcttaatt
    42181 ttcctgggcc aaaatttagc accgcgttgc ctttacccgc ttgcccctgg gtcagcacac
    42241 tcagtcgtcc gctgatcaga gcgttctcaa gccctgcctg ccagttgtcg actttgacac
    42301 ccagtcggcc actcaaagga aaacctgcat acggccaact ccagcgacca tcgcttacgg
    42361 tcaattgttg acgagtaatt tgccacggca aatcgagcaa cggatcgccg ttatcccgtg
    42421 ccagcacaat caattgcccg ctattttcct gccagtccag ctctgcatcc accagtgacg
    42481 gttcctgcgg caagtttaac gtcgcagtag catgcccact tacaggaagt ccatccggca
    42541 cgagcggcat agtaaattcc cccaccagtt taaccggcgg ctgattttca aacgcgacga
    42601 catccagttc gctgactgta agttgttgcc ctttcagctg gccttgaaat ttaacttttt
    42661 cgccctgata acgcagttgc tggatatcag aggttaatgc gagagagagt tttccctgcc
    42721 attcctgcca gggagaaaaa atcagtttat cgatattgat ccaggtgtta ggcagcatgg
    42781 actgccactg cgcgagggtt tttggagcag ctggcgattg ctccgtctgc ggcaatttcg
    42841 ccagacaagc agaatcaagt tctaccgtgc cgacgttcaa taaccagcgg ctgggatgtg
    42901 aaaggctggc gttggtgata tgcgcaagct gacaatctcc caccagataa cggagatcgg
    42961 gaatgattaa acctttacgc gtaatgcgtg ggctttcatc taatgcaata cgtgtcccga
    43021 gcggtagcca gatgcccgcc agagtgggaa cccacagccc gagcgtcatc aacagcgtca
    43081 acggcacaag aataatcagt aataacagcg cgagaacggc tttatattta cccagcatgg
    43141 gtagttaata tcctgattta gcgaaaaatt aagcattcaa tacgggtatt gtggcatgtt
    43201 taaccgttca gttgaaggtt gcgcctacac taagcatagt tgttgatgaa tttttcaata
    43261 tcgccatagc tttcaattaa atttgaaatt ttgtaaaata tttttagtgg cttaaatgtg
    43321 attcaacatc actggagaaa gtcttatgaa actcgccgtt tatagcacaa aacagtacga
    43381 caagaagtac ctgcaacagg tgaacgagtc ctttggcttt gagctggaat tttttgactt
    43441 tctgctgacg gaaaaaaccg ctaaaactgc caatggctgc gaagcggtat gtattttcgt
    43501 aaacgatgac ggcagccgcc cggtgctgga agagctgaaa aagcacggcg ttaaatatat
    43561 cgccctgcgc tgtgccggtt tcaataacgt cgaccttgac gcggcaaaag aactggggct
    43621 gaaagtagtc cgtgttccag cctatgatcc agaggccgtt gctgaacacg ccatcggtat
    43681 gatgatgacg ctgaaccgcc gtattcaccg cgcgtatcag cgtacccgtg acgctaactt
    43741 ctctctggaa ggtctgaccg gatttactat gtatggcaaa acggcaggcg ttatcggtac
    43801 cggtaaaatc ggtgtggcga tgctgcgcat tctgaaaggt tttggtatgc gtctgctggc
    43861 gttcgatccg tatccaagtg cagcggcgct ggaactcggt gtggagtatg tcgatctgcc
    43921 aaccctgttc tctgaatcag acgttatctc tctgcactgc ccgctgacac cggaaaacta
    43981 ccatctgttg aacgaagccg ccttcgatca gatgaaaaat ggcgtgatga tcgtcaatac
    44041 cagtcgcggt gcattgattg attctcaggc agcaattgaa gcgctgaaaa atcagaaaat
    44101 tggttcgttg ggtatggacg tgtatgagaa cgaacgcgat ctgttctttg aagacaaatc
    44161 caacgacgtg atccaggatg acgtgttccg tcgtctgtct gcctgccaca acgtgctgtt
    44221 taccgggcac caggcattcc tgacagcaga agctctgacc agtatttctc agactacgct
    44281 gcaaaactta agcaatctgg aaaaaggcga aacctgcccg aacgaactgg tttaatcttg
    44341 ccgctcccct gcaatccagg ggagctgatt cagataatcc ccaatgacct ttcattctct
    44401 attcttaaaa tagtcctgag tcagaaactg taattgagaa ccacaatgaa gaaactagcc
    44461 gcgtttgttg cgctaagcct gctgatggcg ggatgtgtaa gtaatgacaa aattgctgtt
    44521 acgccagaac agctacagca tcatcgcttt gtgctggaaa gcgtaaacgg taagcccgtg
    44581 accagcgata aaaatccgcc agaaatcagc tttggtgaaa aaatgatgat ttccggcagc
    44641 atgtgtaacc gctttagcgg tgaaggcaaa ctgtctaatg gtgaactgac agccaaaggg
    44701 ctggcaatga cccgtatgat gtgcgctaac ccgcagctta atgaactcga taacaccatt
    44761 agcgaaatgc tgaaagaagg tgcacaagtg gatctgaccg cgaaccagtt aacgctggcg
    44821 accgcgaaac agacattaac ttataagctg gcggatttaa tgaattaata gctgccacag
    44881 ctcccggcgg caagtgactg ttcgctacag cgtttgccgt tgggtaatgc acacatccca
    44941 atcgccgtac catccagttg acgggcaaca gaaagcgaac cgccgatcat tgcacaattt
    45001 gcttctccac tactggacat cgacgctttt aaacctggcg ctacgtgcgc cgcagtcgcc
    45061 tgctgaacag gttcactact acacgccgac aacaataaag cggcacaccc tacccaaaac
    45121 gctgctcgca tctcttcttc ctctgatctt caagccaaac gacaccgcca taaataatag
    45181 gcagcacaga gggcggcgtc gagagctgtc ctgcgcgttg ccccgcattt ttactttttt
    45241 atggctattt ttttgccctc tgtttgatca aaacattcat tacgctgatg tgggggacac
    45301 aaaagcgaaa atgcagaaga aagccatttg ctaaaattga aggattacta ctgggcgcgc
    45361 agcaatttcg tgcgcccctc atttgcgcaa tgtaagggtg tcatatgatt actattgacg
    45421 gtaatggcgc ggttgcttcg gtggcgtttc gcaccagtga agttatcgcc atctacccta
    45481 ttacccccag ttccacgatg gcagaacagg ctgatgcctg ggccggaaac ggcttgaaga
    45541 acgtttgggg agacacacca cgcgtggttg aaatgcagtc ggaagcgggt gctatcgcta
    45601 ccgtgcatgg cgctttgcag acgggtgccc tttcaacatc gtttacgtca tcgcagggtt
    45661 tgctgctgat gatcccgacg ctgtacaaac tggcaggcga actcacaccg tttgtcctgc
    45721 atgtagcggc acgtaccgtt gccacacatg cactctctat ttttggcgat cattccgacg
    45781 tcatggcggt gcgccagacg ggttgcgcga tgttgtgtgc ggcaaacgtc caggaagcgc
    45841 aagactttgc tctcatttcg cacatcgcga cgctgaaaag ccgcgtgcca tttattcatt
    45901 tctttgatgg tttccgcacg tcccacgaaa tcaacaaaat tgtcccgctg gccgatgaca
    45961 cgattcttga tctcatgccg caggccgaaa ttgatgctca tcgcgcccgg gcactcaacc
    46021 cggaacatcc ggtgatccgc ggtacgtccg ccaatcctga cacttatttc cagtctcgcg
    46081 aagccaccaa cccatggtac gacgcggtct atgaccatgt tgaacaggcg atgaatgatt
    46141 tctctgccgc gacaggtcgt cagtatcagc cgtttgaata ttacgggcat ccgcaagcgg
    46201 aacgggtgat tatcctgatg ggctctgcca ttggcacctg tgaagaagtg gttgatgaat
    46261 tgctaacccg tggcgaaaaa gtcggcgtgc tgaaagttcg cctgtaccgc cccttctcag
    46321 ccaaacactt gctacaaact ctaccgggat ccgtacgtag cgtggcggta ctggacagaa
    46381 ccaaagaacc cggtgcccag gcagaaccgc tctatctgga tgtgatgacc gcactggcag
    46441 aagcctttaa taatggcaag cgcgaaactc tcccccgtgt cattggtggg cgctatggtc
    46501 tttcatccaa agaatttggc ccggactgtg tactggcggt atttgccgag ctcaacgcgg
    46561 ctaaaccgaa agcgcgcttt acggttggta tttacgatga tgtaaccaat ctgtccctgc
    46621 cgttgccgga aaacacccag ccaaactcgg cgaaacttga agccttgttt tatggccttg
    46681 gtagtgatgg cagcgtttcc gcgaccaaaa acaatatcaa gattatcggt aattccacgc
    46741 cgtggtacgc acagggctat tttgtttacg actccaaaaa ggcgggcggc ctgacggttt
    46801 ctcaccttcg agtgagcgaa cagccgattc gttccgctta tctcatttcc caggctgatt
    46861 ttgttggctg ccaccagttg cagtttatcg ataaatatca gatggctgag cgtttaaaac
    46921 ctggcggcat tttcctgctc aacacaccgt acagcgcaga tgaagtgtgg tcgcgcttgc
    46981 cgcaagaagt tcaggccgtg ttaaaccaga aaaaagcgcg cttctatgtg attaacgcgg
    47041 cgaaaatcgc ccgcgaatgt ggcctggcgg cccgtattaa taccgtcatg cagatggctt
    47101 ttttccatct gacgcaaatt ctgcctggcg atagcgccct cgcagaattg cagggtgcga
    47161 ttgccaaaag ttacagtagc aaaggccagg atctggtgga acgcaattgg caggctctgg
    47221 cgctggcgcg tgaatccgta gaagaagttc cgttgcaacc ggtaaatccg cacagcgcca
    47281 atcgaccgcc agtggtttcc gatgccgctc ctgatttcgt gaaaaccgta accgctgcga
    47341 tgctcgccgg gcttggtgac gccctccccg tttcggcgct gccgccagac ggctcctggc
    47401 cgatgggcac tacgcgctgg gaaaaacgta atattgccga agagatcccc acctggaaag
    47461 aggaactctg tacccaatgt aaccactgcg ttgccgcttg cccacactca gctattcgcg
    47521 caaaagtggt gccgcctgaa gcgatggaaa acgcccctgc cagcctgcat tcgctggatg
    47581 tgaaatcgcg tgatatgcgc gggcagaaat atgtcttgca ggtggcaccg gaagattgca
    47641 ccggttgtaa cctgtgcgtc gaagtttgcc cggcgaaaga ccgtcagaat ccagagatta
    47701 aagccatcaa tatgatgtct cgcctggaac atgtcgaaga agagaaaatc aattacgatt
    47761 tcttcctcaa cctgccagaa atcgaccgta gcaaactgga acgtattgat attcgtacat
    47821 cgcagctgat tacaccgctg tttgaatatt caggtgcttg ctccggttgt ggcgagacgc
    47881 cgtatattaa attgctgact cagctctatg gcgaccggat gttgatcgct aacgccaccg
    47941 gctgttcttc aatttatggc ggtaacctgc cctctacacc gtataccacc gatgccaacg
    48001 gtcgtgggcc ggcatgggcg aactctctat ttgaagataa tgccgaattt ggccttggtt
    48061 tccgcctgac ggtcgatcaa caccgtgtcc gcgtgctgcg tctgctggat caatttgccg
    48121 ataaaatccc ggcggaatta ctgacggcgt tgaaatcaga cgctacgcca gaggttcgtc
    48181 gagaacaggt cgcagcttta cgccagcaac tcaacgatgt tgccgaagca catgaactgc
    48241 tgcgtgacgc agatgcactg gtggaaaaat caatctggct gattggtggc gacggctggg
    48301 cttacgatat cggctttggc ggtctggatc atgtattgag tttgacggaa aacgtcaaca
    48361 ttctggtgct ggatacgcaa tgctattcca acaccggtgg tcaggcgtcg aaagcgacac
    48421 cgctgggtgc agtaactaag ttcggcgagc acggcaaacg taaagcgcgt aaagatcttg
    48481 gcgtcagtat gatgatgtac ggtcatgttt atgtggcgca gatttctctc ggcgcgcagc
    48541 tgaaccagac ggtgaaagcg attcaggaag cggaagcgta tccggggcca tcgctgatca
    48601 ttgcttatag cccgtgtgaa gagcatggtt acgatctggc actcagccat gaccagatgc
    48661 gccaactcac agctaccggc ttctggccgc tatatcgctt cgatcctcgt cgtgccgatg
    48721 aaggcaaact gccgctggcg ctggattcac gcccgccgtc agaagcactg gaagaaacgt
    48781 tacttcacga gcaacgtttc cgtcgtctga attcgcagca gccagaagtg gcagaacagt
    48841 tatggaaaga tgctgcagct gatttgcaaa aacgctatga cttcctggca caaatggccg
    48901 gaaaagcgga aaaaagcaac accgattaaa tgctctggat aaggattatc caattctaaa
    48961 aaaaaagccc ggacgactgt tcgggcttgt ctttttatat gttggaaaat cagtggcaat
    49021 gcaatggccc aacagaaaat aaaatctgtg acaataaagg catat
//
LOCUS       DAZKHF020000006        48466 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2201S31241
            SAMN43267800-rid25106413.denovo.006, whole genome shotgun sequence.
ACCESSION   DAZKHF020000006 DAZKHF020000000
VERSION     DAZKHF020000006.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267800
            Sequence Read Archive: SRR30307263
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 48466)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 48466)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 48466)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350581.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350581.2
            Genome Coverage       :: 151x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:21:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,652
            CDSs (total)                      :: 5,557
            Genes (coding)                    :: 5,320
            CDSs (with protein)               :: 5,320
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 237
            CDSs (without protein)            :: 237
            Pseudo Genes (ambiguous residues) :: 0 of 237
            Pseudo Genes (frameshifted)       :: 102 of 237
            Pseudo Genes (incomplete)         :: 130 of 237
            Pseudo Genes (internal stop)      :: 52 of 237
            Pseudo Genes (multiple problems)  :: 41 of 237
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..48466
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267800-rid25106413.denovo.006"
                     /serotype="O 26:H11"
                     /isolate="2201S31241"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-01"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            168..1385
                     /gene="alaA"
                     /locus_tag="ACHOSC_RS01245"
                     /old_locus_tag="pgaptmp_000249"
     CDS             168..1385
                     /gene="alaA"
                     /locus_tag="ACHOSC_RS01245"
                     /old_locus_tag="pgaptmp_000249"
                     /EC_number="2.6.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine transaminase AlaA"
                     /protein_id="HGU4546263.1"
                     /translation="MSPIEKSSKLENVCYDIRGPVLKEAKRLEEEGNKVLKLNIGNPA
                     PFGFDAPDEILVDVIRNLPTAQGYCDSKGLYSARKAIMQHYQARGMRDVTVEDIYIGN
                     GVSELIVQAMQALLNSGDEMLVPAPDYPLWTAAVSLSSGKAVHYLCDESSDWFPDLDD
                     IRAKITPRTRGIVIINPNNPTGAVYSKELLMEIVEIARQHNLIIFADEIYDKILYDDA
                     EHHSIAPLAPDLLTITFNGLSKTYRVAGFRQGWMVLNGPKKHAKGYIEGLEMLASMRL
                     CANVPAQHAIQTALGGYQSISEFITPGGRLYEQRNRAWELINDIPGVSCVKPRGALYM
                     FPKIDAKRFNIHDDQKMVLDFLLQEKVLLVQGTAFNWPWPDHFRIVTLPRVDDIELSL
                     SKFARFLSGYHQL"
     gene            1469..2068
                     /gene="yfbR"
                     /locus_tag="ACHOSC_RS01250"
                     /old_locus_tag="pgaptmp_000250"
     CDS             1469..2068
                     /gene="yfbR"
                     /locus_tag="ACHOSC_RS01250"
                     /old_locus_tag="pgaptmp_000250"
                     /EC_number="3.1.3.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0002953 - 5'-deoxynucleotidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5'-deoxynucleotidase"
                     /protein_id="HGU4546264.1"
                     /translation="MKQSHFFAHLSRLKLINRWPLMRNVRTENVSEHSLQVAMVAHAL
                     AAIKNRKFGGNVNAERIALLAMYHDASEVLTGDLPTPVKYFNSQIAQEYKAIEKIAQQ
                     KLVDMVPEELRDIFAPLIDEHAYSDEEKSLVKQADALCAYLKCLEELAAGNNEFLLAK
                     TRLEATLEARRSQEMDYFMEVFVPSFHLSLDEISQDSPL"
     gene            complement(2127..3959)
                     /locus_tag="ACHOSC_RS01255"
                     /old_locus_tag="pgaptmp_000251"
     CDS             complement(2127..3959)
                     /locus_tag="ACHOSC_RS01255"
                     /old_locus_tag="pgaptmp_000251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SLC13 family permease"
                     /protein_id="HGU4546265.1"
                     /translation="MNGELIWVLSLLAVAIVLFATGRVRMDAVALFVIVAFALSGTLT
                     VPEVFSGFSDPNVVLIAALFIIGDGLVRTGVATVMGTWLVKVAGNSEIKMLVLLMLTV
                     AGLGAFMSSTGVVAIFIPVVLSVAMRMQTSPSRLMMPLSFAGLISGMMTLVATPPNLV
                     VNSELLREGYHGFSFFSVTPIGLVVLVLGILYMLVMRFMLKGDTQTPQREGWTRRTFR
                     DLIREYRLTGRARRLAIRPGSPMIGQRLDDLKLRERYGANVIGVERWRRFRRVIVNVN
                     GVSEFRARDVLLIDMSAADVDLRQFCSEQLLEPMVLRGEYFSDQALDVGMAEISLIPE
                     SELIGKSVREIGFRTRYGLNVVGLKRNGVALEGSLADEPLLLGDIILVVGNWKLIGML
                     AKQGRDFVALNLPEEVSEASPAHSQAPHAIFCLVLMVALMLTDEIPNPVAAIIACLLM
                     GKFRCIDAESAYKSIHWPSIILIVGMMPFAVALQKTGGVALAVKGLMDIGGGYGPHMM
                     LGCLFVLSAVIGLFISNTATAVLMAPIALAAAKTMGVSPYPFAMVVAMAASAAFMTPV
                     SSPVNTLVLGPGNYSFSDFVKLGVPFTIIVMAVCVVMIPMLFPF"
     gene            complement(4046..4696)
                     /gene="hxpA"
                     /locus_tag="ACHOSC_RS01260"
                     /old_locus_tag="pgaptmp_000252"
     CDS             complement(4046..4696)
                     /gene="hxpA"
                     /locus_tag="ACHOSC_RS01260"
                     /old_locus_tag="pgaptmp_000252"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hexitol phosphatase HxpA"
                     /protein_id="HGU4546266.1"
                     /translation="MRCKGFLFDLDGTLVDSLPAVERAWSNWARRHGLAPEEVLAFIH
                     GKQAITSLRHFMAGKSEADIAAEFTRLEHIEATETEGITALPGAIALLNHLNKAGIPW
                     AIVTSGSMPVARARHKIAGLPAPEVFVTAERVKRGKPEPDAYLLGAQLLGLAPQECVV
                     VEDAPAGVLSGLAAGCHVIAVNAPADTPRLNEVDLVLHSLEQITVTKQPNGDVIIQ"
     gene            complement(4707..5201)
                     /locus_tag="ACHOSC_RS01265"
                     /old_locus_tag="pgaptmp_000253"
     CDS             complement(4707..5201)
                     /locus_tag="ACHOSC_RS01265"
                     /old_locus_tag="pgaptmp_000253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007371080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfbU family protein"
                     /protein_id="HGU4546267.1"
                     /translation="MEMTNAQRLILSNQYKMMTMLDPANAERYRRLQTIIERGYGLQM
                     RELDREFGELKEETCRTIIDIMEMYHALHVSWSNLQDQQSIDERRVTFLGFDAATEAR
                     YLGYVRFMVNVEGRYTHFDAGTHGFNAQTPMWEKYQRMLNVWHACPRQYHLSANEINQ
                     IINA"
     gene            complement(5284..5739)
                     /gene="yfbV"
                     /locus_tag="ACHOSC_RS01270"
                     /old_locus_tag="pgaptmp_000254"
     CDS             complement(5284..5739)
                     /gene="yfbV"
                     /locus_tag="ACHOSC_RS01270"
                     /old_locus_tag="pgaptmp_000254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminus macrodomain insulation protein YfbV"
                     /protein_id="HGU4546268.1"
                     /translation="MSTPDNRSVNFFSLFRRGQHYSKTWPLEKRLAPVFVENRVIKMT
                     RYAIRFMPPIAVFTLCWQIALGGQLGPAVATALFALSLPMQGLWWLGKRSVTPLPPAI
                     LNWFYEVRGKLQESGQVLAPVEGKPDYQALADTLKRAFKQLDKTFLDDL"
     gene            6077..7279
                     /gene="ackA"
                     /locus_tag="ACHOSC_RS01275"
                     /old_locus_tag="pgaptmp_000255"
     CDS             6077..7279
                     /gene="ackA"
                     /locus_tag="ACHOSC_RS01275"
                     /old_locus_tag="pgaptmp_000255"
                     /EC_number="2.7.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetate kinase"
                     /protein_id="HGU4546269.1"
                     /translation="MSSKLVLVLNCGSSSLKFAIIDAVNGEEYLSGLAECFHLPEARI
                     KWKMDGNKQEAALGAGAAHSEALNFIVNTILAQKPELSAQLTAIGHRIVHGGEKYTSS
                     VVIDESVIQGIKDAASFAPLHNPAHLIGIEEALKSFPQLKDKNVAVFDTAFHQTMPEE
                     SYLYALPYNLYKEHGIRRYGAHGTSHFYVTQEAAKMLNKPVEELNIITCHLGNGGSVS
                     AIRNGKCVDTSMGLTPLEGLVMGTRSGDIDPAIIFHLHDTLGMSVDAINKLLTKESGL
                     LGLTEVTSDCRYVEDNYATKEDAKRAMDVYCHRLAKYIGAYTALMDGRLDAVVFTGGI
                     GENAAMVRELSLGKLGVLGFEVDHERNLAARFGKSGFINKEGTRPAVVIPTNEELVIA
                     QDASRLTA"
     gene            7354..9498
                     /gene="pta"
                     /locus_tag="ACHOSC_RS01280"
                     /old_locus_tag="pgaptmp_000256"
     CDS             7354..9498
                     /gene="pta"
                     /locus_tag="ACHOSC_RS01280"
                     /old_locus_tag="pgaptmp_000256"
                     /EC_number="2.3.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016407 - acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acetyltransferase"
                     /protein_id="HGU4546270.1"
                     /translation="MSRIIMLIPTGTSVGLTSVSLGVIRAMERKGVRLSVFKPIAQPR
                     TGGDAPDQTTTIVRANSSTTTAAEPLKMSYVEGLLSSNQKDVLMEEIVANYHANTKDA
                     EVVLVEGLVPTRKHQFAQSLNYEIAKTLNAEIVFVMSQGTDTPEQLKERIELTRNSFG
                     GAKNTNITGVIVNKLNAPVDEQGRTRPDLSEIFDDSSKAKVNNVDPAKLQESSPLPVL
                     GAVPWSFDLIATRAIDMARHLNATIINEGDINTRRVKSVTFCARSIPHMLEHFRAGSL
                     LVTSADRPDVLVAACLAAMNGVEIGALLLTGGYEMDARISKLCERAFATGLPVFMVNT
                     NTWQTSLSLQSFNLEVPVDDHERIEKVQEYVANYINADWIESLTATSERSRRLSPPAF
                     RYQLTELARKAGKRIVLPEGDEPRTVKAAAICAERGIATCVLLGNPAEINRVAASQGV
                     ELGAGIEIVDPEVVRESYVGRLVELRKNKGMTETVAREQLEDNVVLGTLMLEQDEVDG
                     LVSGAVHTTANTIRPPLQLIKTAPGSSLVSSVFFMLLPEQVYVYGDCAINPDPTAEQL
                     AEIAIQSADSAAAFGIEPRVAMLSYSTGTSGAGSDVEKVREATRLAQEKRPDLMIDGP
                     LQYDAAVMADVAKSKAPNSPVAGRATVFIFPDLNTGNTTYKAVQRSADLISIGPMLQG
                     MRKPVNDLSRGALVDDIVYTIALTAIQSAQQQ"
     gene            9688..11208
                     /gene="yfcC"
                     /locus_tag="ACHOSC_RS01285"
                     /old_locus_tag="pgaptmp_000257"
     CDS             9688..11208
                     /gene="yfcC"
                     /locus_tag="ACHOSC_RS01285"
                     /old_locus_tag="pgaptmp_000257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416801.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative basic amino acid antiporter YfcC"
                     /protein_id="HGU4546271.1"
                     /translation="MSATTESKPTRRWAMPDTLVIIFFVAILTSLATWVVPVGMFDSQ
                     EVQYQVDGQTKTRKVVDPHSFRILTNEAGEPEYHRVQLFTTGDERPGLMNFPFEGLTS
                     GSKYGTAVGIIMFMLVIGGAFGIVMRTGTIDNGILALIRHTRGNEILFIPALFILFSL
                     GGAVFGMGEEAVAFAIIIAPLMVRLGYDSITTVLVTYIATQIGFASSWMNPFCVVVAQ
                     GIAGVPVLSGSGLRIVVWVIATLIGLIFTMVYASRVKKNPLLSRVHESDRFFREKQAD
                     VEQRPFTFGDWLVLIVLTAVMVWVIWGVIVNAWFIPEIASQFFTMGLVIGIIGVVFRL
                     NGMTVNTMASSFTEGARMMIAPALLVGFAKGILLLVGNGEAGDASVLNTILNSIANAI
                     SGLDNAVAAWFMLLFQAVFNFFVTSGSGQAALTMPLLAPLGDLVGVNRQVTVLAFQFG
                     DGFSHIIYPTSASLMATLGVCRVDFRNWLKVGATLLGLLFIMSSVVVIGAQLMGYH"
     gene            complement(11241..11783)
                     /gene="yfcD"
                     /locus_tag="ACHOSC_RS01290"
                     /old_locus_tag="pgaptmp_000258"
     CDS             complement(11241..11783)
                     /gene="yfcD"
                     /locus_tag="ACHOSC_RS01290"
                     /old_locus_tag="pgaptmp_000258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016817 - hydrolase activity, acting on
                     acid anhydrides [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase YfcD"
                     /protein_id="HGU4546272.1"
                     /translation="MEQRRLASTEWVDIVNEENEVIAQASREQMRAQCLRHRATYIVV
                     HDGMGKILVQRRTETKDFLPGMLDATAGGVVQADEQLLESARREAEEELGIAGVPFAE
                     HGQFYFEDKNCRVWGALFSCVSHGPFALQEDEVSEVCWLTPEEITARCDEFTPDSLKA
                     LALWMKRNAKNEAVETETAE"
     gene            complement(11841..12392)
                     /gene="yfcE"
                     /locus_tag="ACHOSC_RS01295"
                     /old_locus_tag="pgaptmp_000259"
     CDS             complement(11841..12392)
                     /gene="yfcE"
                     /locus_tag="ACHOSC_RS01295"
                     /old_locus_tag="pgaptmp_000259"
                     /EC_number="3.1.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311211.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphodiesterase"
                     /protein_id="HGU4546273.1"
                     /translation="MKLMFASDIHGSLPATERVLELFAQSGAQWLVILGDVLNHGPRN
                     ALPEGYAPAKVAERLNEVAHKVIAVRGNCDSEVDQMLLHFPITAPWQQVLLEKQRLFL
                     THGHLFGPENLPALNQNDVLVYGHTHLPVAEQRGEIFHFNPGSVSIPKGGNPASYGML
                     DNDVLSVIALNDQSIIAQVAINP"
     gene            complement(12448..13092)
                     /gene="yfcF"
                     /locus_tag="ACHOSC_RS01300"
                     /old_locus_tag="pgaptmp_000260"
     CDS             complement(12448..13092)
                     /gene="yfcF"
                     /locus_tag="ACHOSC_RS01300"
                     /old_locus_tag="pgaptmp_000260"
                     /EC_number="2.5.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione transferase"
                     /protein_id="HGU4546274.1"
                     /translation="MSKPAITLWSDAHFFSPYVLSAWVALQEKGLSFHIKTIDLDSGE
                     HLQPTWQGYGQTRRVPLLQIDDFELSESSAIAEYLEDRFAPPTWERIYPLDLENRARA
                     RQIQAWLRSDLMPIREERPTDVVFAGAKKAPLTAEGKASAEKLFAMAEHLLVLGQPNL
                     FGEWCIADTDLALMINRLVLHGDEVPERLVDYATFQWQRASVQRFIALSAKQSG"
     gene            13228..13875
                     /gene="yfcG"
                     /locus_tag="ACHOSC_RS01305"
                     /old_locus_tag="pgaptmp_000261"
     CDS             13228..13875
                     /gene="yfcG"
                     /locus_tag="ACHOSC_RS01305"
                     /old_locus_tag="pgaptmp_000261"
                     /EC_number="1.8.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GSH-dependent disulfide bond oxidoreductase"
                     /protein_id="HGU4546275.1"
                     /translation="MIDLYFAPTPNGHKITLFLEEAELDYRLIKVDLGKGGQFRPEFL
                     RISPNNKIPAIVDHSPADGGEPLSLFESGAILLYLAEKTGLFLSHETRERAATLQWLF
                     WQVGGLGPMLGQNHHFNHAAPQTIPYAIERYQVETQRLYHVLNKRLENSPWLGGENYS
                     IADIACWPWVNAWTRQRIDLAMYPAVKNWHERIRSRPATGQALLKAQLGDERSDS"
     gene            13932..14294
                     /gene="folX"
                     /locus_tag="ACHOSC_RS01310"
                     /old_locus_tag="pgaptmp_000262"
     CDS             13932..14294
                     /gene="folX"
                     /locus_tag="ACHOSC_RS01310"
                     /old_locus_tag="pgaptmp_000262"
                     /EC_number="5.1.99.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004150 - dihydroneopterin aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0006760 - folic acid-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroneopterin triphosphate 2'-epimerase"
                     /protein_id="HGU4546276.1"
                     /translation="MAQPAAIIRIKNLRLRTFIGIKEEEINNRQDIVINVTIHYPADK
                     ARTSEDINDALNYRTVTKNIIQHVENNRFSLLEKLTQDVLDIAREHHWVTYAEVEIDK
                     LHALRYADSVSMTLSWQR"
     gene            14315..15208
                     /locus_tag="ACHOSC_RS01315"
                     /old_locus_tag="pgaptmp_000263"
     CDS             14315..15208
                     /locus_tag="ACHOSC_RS01315"
                     /old_locus_tag="pgaptmp_000263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01777 family oxidoreductase"
                     /protein_id="HGU4546277.1"
                     /translation="MNIVITGGTGLIGRHLIPRLLELGHQITVVTRNPQKASSVLGPR
                     VTLWQGLADQSNLNGVDAVINLAGEPIADKRWTHEQKERLCQSRWNITQKLVDLINAS
                     DTPPSVLISGSATGYYGDLGEVVVTEEEPPHNEFTHKLCARWEEIACRAQSDKTRVCL
                     LRTGVVLAPDGGILGKMLPPFRLGLGGPIGSGRQYLAWIHIDDMVNGILWLLDNDLRG
                     PFNMVSPYPVRNEQFAHALGQALHRPAILRVPATAIRLLMGESSILVLGGQRALPKRL
                     EEAGFAFRWYDLEEALADVVR"
     gene            complement(15256..16146)
                     /gene="rpnB"
                     /locus_tag="ACHOSC_RS01320"
                     /old_locus_tag="pgaptmp_000264"
     CDS             complement(15256..16146)
                     /gene="rpnB"
                     /locus_tag="ACHOSC_RS01320"
                     /old_locus_tag="pgaptmp_000264"
                     /EC_number="3.1.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination-promoting nuclease RpnB"
                     /protein_id="HGU4546278.1"
                     /translation="MTISTTSTPHDAVFKSFLRHPDTARDFIDIHLPAPLRKLCDLTT
                     LKLEPNSFIDDDLRQYYSDLLWSVKTQEGVGYIYVVIEHQSKPEELMAFRMMRYSIAA
                     MQNHLDAGYKELPLVIPMLFYHGCRSPYPYSLCWLDEFAEPAIARKIYSSAFPLVDIT
                     VVPDDEIMQHRKMALLELIQKHIRQRDLLGLVDQIVSLLVTGNTNDRQLKALFNYVLQ
                     TGDAQRFRAFIGEITERAPQEKEKLMTIADRLREEGAMQGKHEEALRIAQEMLDRGLD
                     RELVMMVTRLSPDDLIAQSH"
     gene            complement(16343..17116)
                     /gene="hisP"
                     /locus_tag="ACHOSC_RS01325"
                     /old_locus_tag="pgaptmp_000265"
     CDS             complement(16343..17116)
                     /gene="hisP"
                     /locus_tag="ACHOSC_RS01325"
                     /old_locus_tag="pgaptmp_000265"
                     /EC_number="7.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine ABC transporter ATP-binding protein
                     HisP"
                     /protein_id="HGU4546279.1"
                     /translation="MSENKLNVIDLHKRYGEHEVLKGVSLQANAGDVISIIGSSGSGK
                     STFLRCINFLEKPSEGSIVVNGQTINLVRDKDGQLKVADKNQLRLLRTRLTMVFQHFN
                     LWSHMTVLENVMEAPIQVLGLSKQEARERAVKYLAKVGIDERAQGKYPVHLSGGQQQR
                     VSIARALAMEPEVLLFDEPTSALDPELVGEVLRIMQQLAEEGKTMVVVTHEMGFARHV
                     STHVIFLHQGKIEEEGAPEQLFGNPQSPRLQQFLKGSLK"
     gene            complement(17124..17840)
                     /locus_tag="ACHOSC_RS01330"
                     /old_locus_tag="pgaptmp_000266"
     CDS             complement(17124..17840)
                     /locus_tag="ACHOSC_RS01330"
                     /old_locus_tag="pgaptmp_000266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HGU4546280.1"
                     /translation="MIEILHEYWKPLLWTDGYRFTGVAITLWLLILSVVIGGVLALFL
                     AIGRVSSNKYIQFPIWLFTYIFRGTPLYVQLLVFYSGMYTLEIVKGTEFLNAFFRSGL
                     NCTVLALTLNTCAYTTEIFAGAIRSVPHGEIEAARAYGFSTFKMYRCIILPSALRIAL
                     PAYSNEVILMLHSTALAFTATVPDLLKIARDINAATYQPFTAFGIAAVLYLIISYVLI
                     SLFRRAEKRWLQHVKPSSTH"
     gene            complement(17837..18523)
                     /gene="hisQ"
                     /locus_tag="ACHOSC_RS01335"
                     /old_locus_tag="pgaptmp_000267"
     CDS             complement(17837..18523)
                     /gene="hisQ"
                     /locus_tag="ACHOSC_RS01335"
                     /old_locus_tag="pgaptmp_000267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012124147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine ABC transporter permease HisQ"
                     /protein_id="HGU4546281.1"
                     /translation="MLYGFSGVILQGALVTLELAISSVVLAVIIGLIGAGGKLSQNRL
                     SGLIFEGYTTLIRGVPDLVLMLLIFYGLQIALNTVTEAMGVGQIDIDPMVAGIITLGF
                     IYGAYFTETFRGAFMAVPKGHIEAATAFGFTRGQVFRRIMFPAMMRYALPGIGNNWQV
                     ILKSTALVSLLGLEDVVKATQLAGKSTWEPFYFAIVCGVIYLVFTTVSNGVLLFLERR
                     YSVGVKRADL"
     gene            complement(18613..19395)
                     /gene="hisJ"
                     /locus_tag="ACHOSC_RS01340"
                     /old_locus_tag="pgaptmp_000268"
     CDS             complement(18613..19395)
                     /gene="hisJ"
                     /locus_tag="ACHOSC_RS01340"
                     /old_locus_tag="pgaptmp_000268"
                     /EC_number="7.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine ABC transporter substrate-binding
                     protein HisJ"
                     /protein_id="HGU4546282.1"
                     /translation="MKKLVLSLSLVLAFSSATAAFAAIPQNIRIGTDPTYAPFESKNS
                     QGELVGFDIDLAKELCKRINTQCTFVENPLDALIPSLKAKKIDAIMSSLSITEKRQQE
                     IAFTDKLYAADSRLVVAKNSDIQPTVESLKGKRVGVLQGTTQETFGNEHWAPKGIEIV
                     SYQGQDNIYSDLTAGRIDAAFQDEVAASEGFLKQPVGKDYKFGGPSVKDEKLFGVGTG
                     MGLRKEDNELREALNKAFAEMRADGTYEKLAKKYFDFDVYGG"
     gene            complement(19616..20398)
                     /gene="argT"
                     /locus_tag="ACHOSC_RS01345"
                     /old_locus_tag="pgaptmp_000269"
     CDS             complement(19616..20398)
                     /gene="argT"
                     /locus_tag="ACHOSC_RS01345"
                     /old_locus_tag="pgaptmp_000269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine/arginine/ornithine ABC transporter
                     substrate-binding protein ArgT"
                     /protein_id="HGU4546283.1"
                     /translation="MKKSILALSLLVGLSAAASSYAALPETVRIGTDTTYAPFSSKDA
                     KGDFVGFDIDLGNEMCKRMQVKCTWVASDFDALIPSLKAKKIDAIISSLSITDKRQQE
                     IAFSDKLYAADSRLIAAKGSPIQPTLDSLKGKHVGVLQGSTQEAYANETWRSKGVDVV
                     AYANQDLVYSDLAAGRLDAALQDEVAASEGFLKQPAGKDFAFAGSSVKDKKYFGDGTG
                     VGLRKDDAELTAAFNKALGELRQDGTYDKMAKKYFDFNVYGD"
     gene            complement(20664..21233)
                     /gene="ubiX"
                     /locus_tag="ACHOSC_RS01350"
                     /old_locus_tag="pgaptmp_000270"
     CDS             complement(20664..21233)
                     /gene="ubiX"
                     /locus_tag="ACHOSC_RS01350"
                     /old_locus_tag="pgaptmp_000270"
                     /EC_number="2.5.1.129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005120208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavin prenyltransferase UbiX"
                     /protein_id="HGU4546284.1"
                     /translation="MKRLIVGISGASGAIYGVRLLQVLRDVTDIETHLVMSQAARQTL
                     SLETDFSLREVQALADVTHDARDISASISSGSFQTLGMVILPCSIKTLSGIVHSYTDG
                     LLTRAADVVLKERRPLVLCVRETPLHLGHLRLMTQAAEIGAVIMPPVPAFYHRPQSLD
                     DVINQTVNRVLDQFAITLPEDLFARWQGA"
     gene            complement(21328..22845)
                     /gene="purF"
                     /locus_tag="ACHOSC_RS01355"
                     /old_locus_tag="pgaptmp_000271"
     CDS             complement(21328..22845)
                     /gene="purF"
                     /locus_tag="ACHOSC_RS01355"
                     /old_locus_tag="pgaptmp_000271"
                     /EC_number="2.4.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006820991.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004044 - amidophosphoribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009113 - purine nucleobase biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidophosphoribosyltransferase"
                     /protein_id="HGU4546285.1"
                     /translation="MCGIVGIAGVMPVNQSIYDALTVLQHRGQDAAGIITIDANNCFR
                     LRKANGLVSDVFEARHMQRLQGNMGIGHVRYPTAGSSSASEAQPFYVNSPYGITLAHN
                     GNLTNAHELRKKLFEEKRRHINTTSDSEILLNIFASELDNFRHYPLEADNIFAAIAAT
                     NRLIRGAYACVAMIIGHGMVAFRDPNGIRPLVLGKRDIDENRTEYMVASESVALDTLG
                     FDFLRDVAPGEAIYITEEGQLFTRQCADNPVSNPCLFEYVYFARPDSFIDKISVYSAR
                     VNMGTKLGEKIAREWEDLDIDVVIPIPETSCDIALEIARILGKPYRQGFVKNRYVGRT
                     FIMPGQQLRRKSVRRKLNANRAEFRDKNVLLVDDSIVRGTTSEQIIEMAREAGAKKVY
                     LASAAPEIRFPNVYGIDMPSATELIAHGREVDEIRQIIGADGLIFQDLNDLIDAVRAE
                     NPDIQQFECSVFNGVYVTKDVDQGYLDFLDTLRNDDAKAVQRQNEVENLEMHNEG"
     gene            complement(22882..23370)
                     /gene="cvpA"
                     /locus_tag="ACHOSC_RS01360"
                     /old_locus_tag="pgaptmp_000272"
     CDS             complement(22882..23370)
                     /gene="cvpA"
                     /locus_tag="ACHOSC_RS01360"
                     /old_locus_tag="pgaptmp_000272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin V production protein"
                     /protein_id="HGU4546286.1"
                     /translation="MVWIDYAIIAVIAFSSLVSLIRGFVREALSLVTWGCAFFVASHY
                     YTYLSVWFTGFEDELVRNGIAIAVLFIATLIVGAIVNFVIGQLVEKTGLSGTDRVLGV
                     CFGALRGVLIVAAILFFLDSFTGVSKSEDWSKSQLIPQFSFIIRWFFDYLQSSSSFLP
                     RA"
     gene            complement(23629..24291)
                     /gene="dedD"
                     /locus_tag="ACHOSC_RS01365"
                     /old_locus_tag="pgaptmp_000273"
     CDS             complement(23629..24291)
                     /gene="dedD"
                     /locus_tag="ACHOSC_RS01365"
                     /old_locus_tag="pgaptmp_000273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416817.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030428 - cell septum [Evidence IEA];
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA];
                     GO_process: GO:0032506 - cytokinetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein DedD"
                     /protein_id="HGU4546287.1"
                     /translation="MASKFQNRLVGTIVLVALGVIVLPGLLDGQKKHYQDEFAAIPLV
                     PKAGDRDEPDMMPAATQALPTQPPEGAAEEVRAGDAAAPSLDPTTIAANNTEFEPEPA
                     PVAPPKSKPVEPPKPKVEAPPAPKPEPKPVVEEKAAPTGKAYVVQLGALKNADKVNEI
                     VGKLRGAGYRVYTSPSTPVQGKITRILVGPDASKDKLKGSLGELKQLSGLSGVVMGYT
                     PN"
     gene            complement(24281..25549)
                     /gene="folC"
                     /locus_tag="ACHOSC_RS01370"
                     /old_locus_tag="pgaptmp_000274"
     CDS             complement(24281..25549)
                     /gene="folC"
                     /locus_tag="ACHOSC_RS01370"
                     /old_locus_tag="pgaptmp_000274"
                     /EC_number="6.3.2.12"
                     /EC_number="6.3.2.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004326 - tetrahydrofolylpolyglutamate
                     synthase activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_process: GO:0009396 - folic acid-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional tetrahydrofolate
                     synthase/dihydrofolate synthase"
                     /protein_id="HGU4546288.1"
                     /translation="MIIKRTPQAASPLASWLSYLENLHSKTIDLGLERVSQVAARLGV
                     LKPAPFVFTVAGTNGKGTTCRTLESILMAAGYKVGVYSSPHLVRYTERVRVQGQELPE
                     SAHTASFAEIESARGDISLTYFEYGTLSALWLFKQAQLDVVILEVGLGGRLDATNIVD
                     ADVAVVTSIALDHTDWLGPDRESIGREKAGIFRSEKPAIVGEPEMPSTIADVAQEKGA
                     LLQRRGVEWNYSVTDHDWAFSDAHGTLENLPLPLVPQPNAATALAALRASGLEVSENA
                     IRDGIASAILPGRFQIVSESPRVIFDVAHNPHAAEYLTGRMKALPKNGRVLAVIGMLH
                     DKDIAGTLAWLKSVVDDWYCAPLEGPRGATAEQLLEHLGNGKSFDSVAQAWDAAMADA
                     KAEDTVLVCGSFHTVAHVMEVIDARRSGGK"
     gene            complement(25619..26533)
                     /gene="accD"
                     /locus_tag="ACHOSC_RS01375"
                     /old_locus_tag="pgaptmp_000275"
     CDS             complement(25619..26533)
                     /gene="accD"
                     /locus_tag="ACHOSC_RS01375"
                     /old_locus_tag="pgaptmp_000275"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase, carboxyltransferase
                     subunit beta"
                     /protein_id="HGU4546289.1"
                     /translation="MSWIERIKSNITPTRKASIPEGVWTKCDSCGQVLYRAELERNLE
                     VCPKCDHHMRMTARNRLHSLLDEGSLVELGSELEPKDVLKFRDSKKYKDRLASAQKET
                     GEKDALVVMKGTLYGMPVVAAAFEFAFMGGSMGSVVGARFVRAVEQALEDNCPLICFS
                     ASGGARMQEALMSLMQMAKTSAALAKMQERGLPYISVLTDPTMGGVSASFAMLGDLNI
                     AEPKALIGFAGPRVIEQTVREKLPPGFQRSEFLIEKGAIDMIVRRPEMRLKLASILAK
                     LMNLPAPNPEAPREGVVVPPVPDQEPEA"
     gene            complement(26689..27348)
                     /locus_tag="ACHOSC_RS01380"
                     /old_locus_tag="pgaptmp_000276"
     CDS             complement(26689..27348)
                     /locus_tag="ACHOSC_RS01380"
                     /old_locus_tag="pgaptmp_000276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HGU4546290.1"
                     /translation="MDLIYFLIDFILHIDVHLAELVAEYGVWVYAILFLILFCETGLV
                     VTPFLPGDSLLFVAGALASLETNDLNVHMMVVLMLIAAIVGDAVNYTIGRLFGDKLFS
                     NPDSKIFRRSYLDKTHQFYERHGGKTIILARFVPIVRTFAPFVAGMGHMSYRHFAAYN
                     VIGALLWVLIFTYAGYFFGTLPFIQSNLKLMIVGIIFVSILPGVFEFIRHKRAAARAA
                     K"
     gene            complement(27431..28243)
                     /gene="truA"
                     /locus_tag="ACHOSC_RS01385"
                     /old_locus_tag="pgaptmp_000277"
     CDS             complement(27431..28243)
                     /gene="truA"
                     /locus_tag="ACHOSC_RS01385"
                     /old_locus_tag="pgaptmp_000277"
                     /EC_number="5.4.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(38-40) synthase TruA"
                     /protein_id="HGU4546291.1"
                     /translation="MSDQQQLPVYKIALGIEYDGSKYYGWQRQNEVRSVQEKLEKALS
                     QVANEPITVFCAGRTDAGVHGTGQVVHFETTAQRKDAAWTLGVNANLPGDIAVRWVKA
                     VPDDFHARFSATARRYRYIIYNHRLRPAVLSKGVTHFYEPLDAERMHRAAQCLLGEND
                     FTSFRAVQCQSRTPWRNVMHINVMRHGPYVVVDIKANAFVHHMVRNIVGSLMEVGAHN
                     QPESWIAELLAAKDRTRAAATAKAEGLYLVAVDYPDRYDLPKPPMGPLFLAD"
     gene            complement(28243..29256)
                     /locus_tag="ACHOSC_RS01390"
                     /old_locus_tag="pgaptmp_000278"
     CDS             complement(28243..29256)
                     /locus_tag="ACHOSC_RS01390"
                     /old_locus_tag="pgaptmp_000278"
                     /EC_number="1.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0008652 - amino acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate-semialdehyde dehydrogenase"
                     /protein_id="HGU4546292.1"
                     /translation="MSEGWNIAVLGATGAVGEALLETLAERQFPVGEIYALARNESAG
                     EQLRFGGKTITVQDAAEFDWTQAQLAFFVAGKEATAAWVEEATNSGCLVIDSSGLFAL
                     EPDVPLVVPEVNPFVLTDYRNRNVIAVPDSLTSQLLAALKPLIDQGGLSRISVTSLIS
                     ASAQGKKAVDALAGQSAKLLNGIPIDEEDFFGRQLAFNMLPLLPDSEGSVREERRIVD
                     EVRKILQDEGLMISASVVQAPVFYGHAQMVNFEALRPLAAEEARDAFAQGEDIVLSEE
                     NEFPTQVGDASGTPHLSVGCVRNDYGMPEQVQFWSVADNVRFGGALMAVKIAEKLVQE
                     YLY"
     gene            complement(29322..30458)
                     /gene="pdxB"
                     /locus_tag="ACHOSC_RS01395"
                     /old_locus_tag="pgaptmp_000279"
     CDS             complement(29322..30458)
                     /gene="pdxB"
                     /locus_tag="ACHOSC_RS01395"
                     /old_locus_tag="pgaptmp_000279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033711 - 4-phosphoerythronate
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-phosphoerythronate dehydrogenase PdxB"
                     /protein_id="HGU4546293.1"
                     /translation="MKILVDENMPYARDLFSRLGEVTAVPGRPIPVAQLADADALMVR
                     SVTKVNESLLAGKPIKFVGTATAGTDHVDEAWLKQAGIGFSAAPGCNAIAVVEYVFSS
                     LLMLAERDGFSLHDRTVGIVGVGNVGRRLQARLEALGIKTLLCDPPRADRGDEGDFRS
                     LDELVQHADILTFHTPLFKDGPYKTLHLADEKLIRSLKPGAILINACRGAVVDNTALL
                     TCLNEGQKLSVVLDVWEGEPELNVELLTKVDIGTPHIAGYTLEGKARGTTQVFEAYSK
                     FIGHEQHVALDTLLPAPEFGRITLHGPLDQPTLKRLVHLVYDVRRDDAPLRKVAGIPG
                     EFDKLRKNYLERREWSSLYVICDDASAASLLCKLGFNAVHHPAR"
     gene            30557..31552
                     /gene="flk"
                     /locus_tag="ACHOSC_RS01400"
                     /old_locus_tag="pgaptmp_000280"
     CDS             30557..31552
                     /gene="flk"
                     /locus_tag="ACHOSC_RS01400"
                     /old_locus_tag="pgaptmp_000280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0010468 - regulation of gene expression
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagella biosynthesis regulator Flk"
                     /protein_id="HGU4546294.1"
                     /translation="MIQPISGPPPGQPPGQGDNLPSGAGNQPLSSQQRTSLESLMTKV
                     TSLTQQQRAELWAGIRHDIGLSGDSPLLSRHFPAAEHNLAQRLLAAQKSHSARQLLAQ
                     LGEYLRLGNNRQAVTDYIRHNFGQTPLNQLSPEQLKTILTLLQEGKMVIPQPQQREAT
                     DRPLLPAEHNALKQLVTKLAAATGEPSKQIWQSMLELSGVKDGELIPAKLFNHLVTWL
                     QARQTLSQQNTPTLESLQMALKQPLDASELAALSAYIQQKYGLSAQSSLSSAQAEDIL
                     NQLYQRRVKGIDPRDMQPLLNPFPPMMDTLQNMATRPALWILLVAIILMLVWLVR"
     gene            complement(31549..32727)
                     /locus_tag="ACHOSC_RS01405"
                     /old_locus_tag="pgaptmp_000281"
     CDS             complement(31549..32727)
                     /locus_tag="ACHOSC_RS01405"
                     /old_locus_tag="pgaptmp_000281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HGU4546295.1"
                     /translation="MTAVSQTETRSSANFSLFRIAFAVFLTYMTVGLPLPVIPLFVHH
                     DLGYGNTMVGIAVGIQFLATVLTRGYAGRLADQYGAKRSALQGMLACGLAGGALLLAA
                     ILPVSAPFKFALLVIGRLILGFGESQLLTGALTWGLGIVGPKHSGKVMSWNGMAIYGA
                     LAVGAPLGLLIHSHYGFAALAITTMVLPLLAWACNGTVRKVPALAGERPSLWSVVGFI
                     WKPGLGLALQGVGFAVIGTFVSLYFASKGWAMAGFTLTAFGGAFVVMRVMFGWMPDRF
                     GGVKVAIVSLLVETVGLLLLWQAPGAWVALAGAALTGAGCSLIFPALGVEVVKRVPSQ
                     VRGTALGGYAAFQDIALGVSGPLAGMLATTFGYSSVFLAGAISAVLGIIVTILSFRRG
                     "
     gene            complement(33011..34231)
                     /gene="fabB"
                     /locus_tag="ACHOSC_RS01410"
                     /old_locus_tag="pgaptmp_000282"
     CDS             complement(33011..34231)
                     /gene="fabB"
                     /locus_tag="ACHOSC_RS01410"
                     /old_locus_tag="pgaptmp_000282"
                     /EC_number="2.3.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase I"
                     /protein_id="HGU4546296.1"
                     /translation="MKRAVITGLGIVSSIGNNQQEVLASLREGRSGITFSQELKDSGM
                     RSHVWGNVKLDTTGLIDRKVVRFMSDASIYAFLSMEQAIADAGLSPEAYQNNPRVGLI
                     AGSGGGSPRFQVFGADAMRGPRGLKAVGPYVVTKAMASGVSACLATPFKIHGVNYSIS
                     SACATSAHCIGNAVEQIQLGKQDIVFAGGGEELCWEMACEFDAMGALSTKYNDTPEKA
                     SRTYDAHRDGFVIAGGGGMVVVEELEHALARGAHIYAEIVGYGATSDGADMVAPSGEG
                     AVRCMKMAMHGVDTPIDYLNSHGTSTPVGDVKELAAIREVFGDKSPAISATKAMTGHS
                     LGAAGVQEAIYSLLMLEHGFIAPSINIEELDEQAAGLNIVTETTDRELTTVMSNSFGF
                     GGTNATLVMRKLKD"
     gene            34390..36396
                     /gene="mnmC"
                     /locus_tag="ACHOSC_RS01415"
                     /old_locus_tag="pgaptmp_000283"
     CDS             34390..36396
                     /gene="mnmC"
                     /locus_tag="ACHOSC_RS01415"
                     /old_locus_tag="pgaptmp_000283"
                     /EC_number="1.5.-.-"
                     /EC_number="2.1.1.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416827.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004808 - tRNA
                     (5-methylaminomethyl-2-thiouridylate)(34)-
                     methyltransferase activity [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional tRNA
                     (5-methylaminomethyl-2-thiouridine)(34)-methyltransferase
                     MnmD/FAD-dependent
                     5-carboxymethylaminomethyl-2-thiouridine(34)
                     oxidoreductase MnmC"
                     /protein_id="HGU4546297.1"
                     /translation="MKHYSIQPANLEFNAEGTPVSRDFDDVYFSNDNGLEETRYVFLG
                     GNQLEVRFPEHPHPLFVVAESGFGTGLNFLTLWQAFNQFREAHPQAQLQRLHFISFEK
                     FPLTRADLALAHQHWPELAPWAEQLQAQWPMPLPGCHRLLLDEGRVTLDLWFGDINEL
                     TSQLDDSLNQKVDAWFLDGFAPAKNPDMWTQNLFNAMARLARPGGTLATFTSAGFVRR
                     GLQDAGFTMQKRKGFGRKREMLCGVMEQTLPLPCSAPWFNRTGSSKREAAIIGGGIAS
                     ALLSLALLRRGWQVTLYCADEAPALGASGNRQGALYPLLSKHDEALNRFFSNAFTFAR
                     RFYDQLPVKFDHDWCGVTQLGWDEKSQHKIAQMLSMDLPAELAVAVEANAVEQITGVA
                     TNCSGITYPQGGWLCPAELTRNVLELAQQQGLQIYYQYQLQNLSRKDDCWLLNFAGDQ
                     QATHSVVVLANGHQISRFSQTSTLPVYSVAGQVSHIPTTPELAELKQVLCYDGYLTPQ
                     NPANQHHCIGASYHRGSEDTAYSEDDQQQNRQRLIDCFPQAQWAKEVDVSDKEARCGV
                     RCATRDHLPMVGNVPDYEATLVEYASLAEQKDEAVSAPVFDDLFMFAALGSRGLCSAP
                     LCAEILAAQMSDEPIPMDASTLAALNPNRLWVRKLLKGKAVKAG"
     gene            complement(36517..36795)
                     /locus_tag="ACHOSC_RS01420"
                     /old_locus_tag="pgaptmp_000284"
     CDS             complement(36517..36795)
                     /locus_tag="ACHOSC_RS01420"
                     /old_locus_tag="pgaptmp_000284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfcL family protein"
                     /protein_id="HGU4546298.1"
                     /translation="MIAEFESRILALIDGMVDHASDDELFASGYLRGHLTLAIAELES
                     GDDHSAQAVHTTVSQSLEKAIGAGELSPRDQALVTDMWENLFQQASQQ"
     gene            complement(36829..37377)
                     /gene="epmC"
                     /locus_tag="ACHOSC_RS01425"
                     /old_locus_tag="pgaptmp_000285"
     CDS             complement(36829..37377)
                     /gene="epmC"
                     /locus_tag="ACHOSC_RS01425"
                     /old_locus_tag="pgaptmp_000285"
                     /EC_number="1.14.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P hydroxylase"
                     /protein_id="HGU4546299.1"
                     /translation="MNSTHHYEQLIEIFNSCFADEFNTRLIKGDDEPIYLPADAEVPY
                     NRIVFAHGFYASAIHEISHWCIAGKARRELVDFGYWYCPDGRDAQTQSQFEDVEVKPQ
                     AFDWLFCVAAGYPFNVSCDNLEGDFEPDRVVFQRRVHAQVMDYLANGIPERPARFIKA
                     LQNYYHTPELTAEQFPWPEALN"
     gene            complement(37377..38186)
                     /locus_tag="ACHOSC_RS01430"
                     /old_locus_tag="pgaptmp_000286"
     CDS             complement(37377..38186)
                     /locus_tag="ACHOSC_RS01430"
                     /old_locus_tag="pgaptmp_000286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HGU4546300.1"
                     /translation="METFNSLFMVSPLLLGVLFFVAMLAGFIDSIAGGGGLLTIPALM
                     AAGMSPANALATNKLQACGGSISATIYFIRRKVVSLSDQKLNIAMTFVGSMSGALLVQ
                     YVQADVLRQILPILVICIGLYFLLMPKLGEEDRQRRMYGLPFALIAGGCVGFYDGFFG
                     PAAGSFYALAFVTLCGFNLAKATAHAKLLNATSNIGGLLLFILGGKVIWATGFVMLVG
                     QFLGARMGSRLVLSKGQKLIRPMIVIVSAVMSAKLLFDSHGQEILHWLGMN"
     gene            complement(38186..39010)
                     /gene="mepA"
                     /locus_tag="ACHOSC_RS01435"
                     /old_locus_tag="pgaptmp_000287"
     CDS             complement(38186..39010)
                     /gene="mepA"
                     /locus_tag="ACHOSC_RS01435"
                     /old_locus_tag="pgaptmp_000287"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-insensitive murein endopeptidase"
                     /protein_id="HGU4546301.1"
                     /translation="MNKTAIALLALLASSASLAATPWQKITQPVPGSAQSIGSFSNGC
                     IVGADTLPIQSEHYQVMRTDQRRYFGHPDLVMFIQRLSSQVSNLGMGTVLIGDMGMPA
                     GGRFNGGHASHQTGLDVDIFLQLPKTRWTSAQLLRPQALDLVSRDGKHVVSSLWKPEI
                     FSLIKLAAQDKNVTRIFVNPAIKQQLCLDAGTDRDWLRKVRPWFQHRAHMHVRLRCPA
                     DSLECEDQPLPPPGDGCGAELQSWFEPPKPGTTKPEKKTPPPLPPSCQALLDEHVI"
     gene            complement(39014..40099)
                     /gene="aroC"
                     /locus_tag="ACHOSC_RS01440"
                     /old_locus_tag="pgaptmp_000288"
     CDS             complement(39014..40099)
                     /gene="aroC"
                     /locus_tag="ACHOSC_RS01440"
                     /old_locus_tag="pgaptmp_000288"
                     /EC_number="4.2.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004107 - chorismate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate synthase"
                     /protein_id="HGU4546302.1"
                     /translation="MAGNTIGQLFRVTTFGESHGLALGCIVDGVPPGIPLTEADLQHD
                     LDRRRPGTSRYTTQRREPDQVKILSGVFEGVTTGTSIGLLIENTDQRSQDYSAIKDVF
                     RPGHADYTYEQKYGLRDYRGGGRSSARETAMRVAAGAIAKKYLAEKFGIEIRGCLTQM
                     GDIPLEIKDWSLVEQNPFFCPDPDKIDALDELMRALKKEGDSIGAKVTVVASGVPAGL
                     GEPVFDRLDADIAHALMSINAVKGVEIGDGFDVVALRGSQNRDEITKDGFQSNHAGGI
                     LGGISSGQQIIAHMALKPTSSITVPGRTINRFGEEVEMITKGRHDPCVGIRAVPIAEA
                     MLAIVLMDHLLRQRAQNADVKTDIPRW"
     gene            complement(40134..41066)
                     /gene="prmB"
                     /locus_tag="ACHOSC_RS01445"
                     /old_locus_tag="pgaptmp_000289"
     CDS             complement(40134..41066)
                     /gene="prmB"
                     /locus_tag="ACHOSC_RS01445"
                     /old_locus_tag="pgaptmp_000289"
                     /EC_number="2.1.1.298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008276 - protein methyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0018364 - peptidyl-glutamine methylation
                     [Evidence IEA];
                     GO_process: GO:0042273 - ribosomal large subunit
                     biogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L3 N(5)-glutamine
                     methyltransferase"
                     /protein_id="HGU4546303.1"
                     /translation="MDKIFVDEAVNELQTIQDMLRWSVSRFSAANIWYGHGTDNPWDE
                     AVQLVLPSLYLPLDIPEDMRTARLTSSEKHRIVERVIRRVNERIPVAYLTNKAWFCGH
                     EFYVDERVLVPRSPIGELINNKFAGLISKQPQHILDMCTGSGCIAIACAYAFPEAEVD
                     AVDISPDALAVAEQNIEEHGLIHNVIPIRSDLFRDLPKVQYDLIVTNPPYVDAEDMSD
                     LPNEYRHEPELGLASGTDGLKLTRRILGNAADYLADDGVLICEVGNSMVHLMEQYPDV
                     PFTWLEFDNGGDGVFMLTKEQLLAAREYFAIYKD"
     gene            41232..41783
                     /gene="smrB"
                     /locus_tag="ACHOSC_RS01450"
                     /old_locus_tag="pgaptmp_000290"
     CDS             41232..41783
                     /gene="smrB"
                     /locus_tag="ACHOSC_RS01450"
                     /old_locus_tag="pgaptmp_000290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease SmrB"
                     /protein_id="HGU4546304.1"
                     /translation="MKKKTTLSEEDQALFRQLMAGTRKIKQDTIVHRPQRKKISEVPV
                     KRLIQEQADASHYFSDEFQPLLNTEGPVKYVRPDVSHFEAKKLRRGDYSPELFLDLHG
                     LTQLQAKQELGALIAACRREHVFCACVMHGHGKHILKQQTPLWLAQHPHVMAFHQAPK
                     EYGGDAALLVLIEVEEWLPPELP"
     gene            complement(41856..42707)
                     /locus_tag="ACHOSC_RS01455"
                     /old_locus_tag="pgaptmp_000291"
     CDS             complement(41856..42707)
                     /locus_tag="ACHOSC_RS01455"
                     /old_locus_tag="pgaptmp_000291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001721620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="StfH/YfcO family fimbrial adhesin"
                     /protein_id="HGU4546305.1"
                     /translation="MHKIRHVICLLVLALCASGIQAATKIATLYVPAGTTSVVAKYRF
                     HLSVLTPQSVEYGTYESNSTAAASLPLVSWTGSPPGPELRMERNNTTLPDSTCPGLEE
                     YDALSPVTAWSCNELVLGVYYDGDLHGCPWIVSSYVESASTMDQRFGAGIFIGPTKVS
                     SDCPTESLAPYDVSWNENYVVHNKVVRLQSTGGVIEQTLPTFLMENGKLCDGSNFDER
                     GAYCRFVAQQMTFSTSGCDNAKVTVMPEPQPITSRQLHDMKLRVDTTSRQPIDSTCRF
                     TYILNMY"
     gene            complement(42709..43248)
                     /locus_tag="ACHOSC_RS01460"
                     /old_locus_tag="pgaptmp_000292"
     CDS             complement(42709..43248)
                     /locus_tag="ACHOSC_RS01460"
                     /old_locus_tag="pgaptmp_000292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HGU4546306.1"
                     /translation="MKTAHIYSPGYALLAGLLLTASTGARAVDNNLHFYGNLLSKSCT
                     LVVDGANLAEIHFPTVSNRDLMVAGQSAHLPVVFKLKDCKGPAGYNVKVTLTGTEDSG
                     QPGFLAVDDTSTAQGVGIGMETLDGKRVDINNTTGATFALTNGNNDINFRAWIQAKSG
                     RDVTIGDFTASLTATFEYI"
     gene            complement(43245..43733)
                     /locus_tag="ACHOSC_RS01465"
                     /old_locus_tag="pgaptmp_000293"
     CDS             complement(43245..43733)
                     /locus_tag="ACHOSC_RS01465"
                     /old_locus_tag="pgaptmp_000293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001709066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HGU4546307.1"
                     /translation="MKKFLLLLLCAGCGAVLAAEDDITFHGTLMSPPACSISGGQTIE
                     VQFRDLIIDSIDGNYGRQKVDYELSCDSDVRDPEWNMTLSWTGTATSYNDSAIETDVP
                     GFGIELQHDGQRFKLNEPLSINATDFSQKPKLEAVPVKAADAVLTDTNFSAYATLRVD
                     YQ"
     gene            complement(43730..44239)
                     /locus_tag="ACHOSC_RS01470"
                     /old_locus_tag="pgaptmp_000294"
     CDS             complement(43730..44239)
                     /locus_tag="ACHOSC_RS01470"
                     /old_locus_tag="pgaptmp_000294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001733155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HGU4546308.1"
                     /translation="MSHKFVAGAGLLVLAGWGISGLAVAGSDIKQVDLTLRILVDGPP
                     PCSVKGSAVKFGNVIINRIDGTNYQQDAKYTLNCGNKVSDDLRMQLKGNTAVINGETV
                     LSTDIAGLGVRIENAADNSLFAVGENNWTPFNVNTQPQLKAVPVKQSGVQLTPSEFNA
                     TMTMVVDYQ"
     gene            complement(44255..45007)
                     /locus_tag="ACHOSC_RS01475"
                     /old_locus_tag="pgaptmp_000295"
     CDS             complement(44255..45007)
                     /locus_tag="ACHOSC_RS01475"
                     /old_locus_tag="pgaptmp_000295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbria/pilus periplasmic chaperone"
                     /protein_id="HGU4546309.1"
                     /translation="MFNLTNTAKIVVPALALLATVVSFSSHASVTPDRTRLVFNESDK
                     SISVTLRNNDPTLPYLAQSWIEDAKGNKISSPLAVLPPVQRIDAMMNGQVKVQGLPDI
                     NKLPADRESVFYFNVREIPPKSNKPNTLQIALQTRIKLFWRPKALENTSMKSPWQHKV
                     TLIRNGQDFTVNNPTPYYVIISNASAKKGGNPPAGFTPLMMEPKTSAPLNAKMDSVPV
                     LTYINDYGARMPLYFICTGNSCQVDEEQSRKG"
     gene            complement(45027..47672)
                     /gene="yfcU"
                     /locus_tag="ACHOSC_RS01480"
                     /old_locus_tag="pgaptmp_000296"
     CDS             complement(45027..47672)
                     /gene="yfcU"
                     /locus_tag="ACHOSC_RS01480"
                     /old_locus_tag="pgaptmp_000296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001112832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial usher protein YfcU"
                     /protein_id="HGU4546310.1"
                     /translation="MPDHSLFRLRILPWCIALAMSGSYSSVWAEDDIQFDSRFLELKG
                     DTKIDLKRFSSQGYVEPGKYNLQVQLNKQPLAEEYDIYWYAGEDDASKSYACLTPELV
                     AQFGLKEDVAKNLQWSHDAKCLKSGQLEGMEIKADLSQSALVISLPQAYLEYTYPDWD
                     PPSRWDDGISGIVADYSINAQTRHEENGGDDSNEISGNGTVGVNLGPWRMRADWQTNY
                     QHTRSNDDDDEFSGDETQKKWEWSRYYAWRALPSLKAKLALGEDYLRSDIFDGFNYVG
                     GSVSTDDQMLPPNLRGYAPDISGVAHTTAKVTVSQMGRVIYETQVPAGPFRIQDLGDS
                     VSGTLHIRIEEQNGQVQEYDISTASMPYLTRPGQVRYKIMMGRPQEWGHHIEGEFFSG
                     AEASWGIANGWSLYGGALGDENYQSAALGIGRDLSTFGAVAFDVTHSHTKLDKDTAYG
                     KGSLDGNSFRVSYSKDFDQLNSRVTFAGYRFSEENFMTMSEYLDASDSGMVRTGNDKE
                     MYTATYNQNFRDAGVSVYLNYTRHTYWDREEQTNYNIMLSHYFNMGSIRNVSISMTGY
                     RYEYDNQADKGMYISLSMPWGDNSTVSYNGNYGSGTDSSQVGYFSRVDDATHYQLNVG
                     TSDKHTSVDGYYSHDGSLAQVDLSANYHEGQYTSAGLSLQGGATLTAHGGALHRTQNM
                     GGTRLLIDADGVADVPVEGNGAAVYTNMFGKAVVSDVNNYYRNQAYIDLNRLPENAEA
                     TQSVVQATLTEGAIGYRKFAVISGQKAMAVLRLSDGSHPPFGAEVKNDNEQTVGLVDD
                     DGNVYLAGVKPGEHMSVFWSGVAHCDINLPDPLPADLFNGLLLPCQHKGNVAPITSPA
                     VKPAIQEQTQRVTPTEPPTSISVNQ"
     gene            complement(47754..48317)
                     /locus_tag="ACHOSC_RS01485"
                     /old_locus_tag="pgaptmp_000297"
     CDS             complement(47754..48317)
                     /locus_tag="ACHOSC_RS01485"
                     /old_locus_tag="pgaptmp_000297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HGU4546311.1"
                     /translation="MSKFVKTAIAAAMVMGVFTSTATIAAGNNGTARFYGTIEDSVCS
                     IVPDDHKLEVDMGDIGAEKLKNNGTTTPKSFQIRLQDCVFDTQETMTTTFTGTVSSAN
                     SGNYYTIFNTDTGAAFNNVSLAIGDSLGTSYKSGMGIDQKIVKDTSTNKGKAKQTLNF
                     KAWLVGAADAPDLGNFEANTTFQITYL"
BASE COUNT        11825 a        13139 c        12129 g        11373 t
ORIGIN      
        1 cacctcatta ttttgtcatt attaagttat taacagcaca atcgagcctt cccctctggc
       61 aaaatcttat tctgcagacc ttcaaaacac cgtcctgggg gagtacattg ttctaagctg
      121 acttccacgg cagggagtgg cgataacagc aaaaaaggtc aagattcatg tcccccattg
      181 aaaaatccag caaattagag aatgtctgtt atgacatccg tggtccggtg ctgaaagaag
      241 caaaacgcct ggaagaagaa ggtaacaagg tactgaaact gaacatcggc aacccagccc
      301 cgttcggttt tgacgcgcca gatgaaatcc tcgttgacgt gatacgcaac ctgcctaccg
      361 ctcaagggta ttgcgattcc aaaggtcttt actccgcgcg taaagccatc atgcagcact
      421 accaggctcg tggcatgcgt gatgttaccg tggaagatat ttacatcggc aatggtgtat
      481 cggagcttat cgttcaggca atgcaggcat tgctgaacag cggggacgaa atgttggttc
      541 ctgcaccaga ttacccacta tggaccgcgg cggtttcgct ttccagcggt aaagcggtgc
      601 attatctttg cgatgaatcc tctgactggt tcccggacct cgatgatatt cgcgctaaaa
      661 ttacgcctcg tacgcgtggg atcgttatta tcaacccaaa taacccaacc ggcgcggtat
      721 attccaaaga gcttttaatg gagattgtgg agattgcacg tcagcataat ctcattatct
      781 tcgccgatga aatttatgac aaaattcttt acgacgacgc tgagcatcac tcaattgcgc
      841 cgctggcacc tgacctgctg accattacct ttaacggact gtcgaaaacg taccgcgttg
      901 caggcttccg tcaggggtgg atggtgttga acgggccgaa aaaacacgcc aaaggctaca
      961 tcgaaggtct ggaaatgctg gcttcaatgc gcctgtgtgc taacgttcct gcgcaacacg
     1021 ccattcagac cgcgctgggt ggttatcaga gcatcagtga atttattacc cctggcggtc
     1081 gtctttatga gcagcgtaac cgcgcgtggg aactgatcaa cgatattccg ggcgtttcct
     1141 gcgtgaaacc tcgtggtgcg ctgtatatgt tcccgaaaat cgacgccaaa cgctttaaca
     1201 ttcacgacga tcagaaaatg gtgctggatt tcctgttgca ggaaaaagtt ctgttggtgc
     1261 aagggacggc attcaactgg ccgtggccgg atcacttccg cattgtcacg ctaccgcgtg
     1321 tcgatgatat cgagctgtct ttgagcaagt tcgcgcgttt cctttctggt tatcatcagc
     1381 tgtaatctta atttcactgc cggagattgc atccggcagc gttatcccgc cacaatgacc
     1441 tgatgatgtc atcatacgta aggtcactat gaaacagagc catttttttg cccatctctc
     1501 ccgcctgaaa ctcattaacc gctggccgct catgcgcaac gtgcggacgg aaaatgtgtc
     1561 cgaacacagt ttgcaggtag cgatggtcgc ccatgcgctg gcagctatca aaaatcggaa
     1621 atttggcggt aatgtcaacg ccgaacgtat cgctttactg gcgatgtacc acgatgcctc
     1681 agaagtgctc accggcgatc tccctactcc ggtgaaatac ttcaattcgc aaatcgctca
     1741 ggagtacaag gctattgaaa aaatcgctca gcaaaaactg gtcgatatgg ttccggaaga
     1801 gctgcgggat atctttgcgc cgttaattga cgagcatgca tatagcgatg aagaaaaatc
     1861 gctggtgaaa caggcagatg cactgtgtgc atatctgaaa tgtctggaag aactcgcggc
     1921 cggaaataat gaattcttgc tggcaaaaac gcgactggaa gcgacgcttg aagcgcgtcg
     1981 cagccaggag atggactact tcatggaagt atttgttccc agcttccatc tttcgctcga
     2041 tgagattagc caggattcac cgctgtaagc agccggagtc tgcgtcgcat caggcaataa
     2101 gcgccggatg cgacatcagg ctcttgtcaa aacggaaaca gcatcgggat catcaccaca
     2161 caaaccgcca tcacgataat ggtgaacggt acccccaact tcacaaagtc actgaagctg
     2221 taatttcccg gacctaaaac cagtgtgtta acaggtgaag aaaccggtgt cataaaggcg
     2281 gcggatgctg ccatcgccac gaccatcgcg aatggataag gcgacacccc catcgttttg
     2341 gcagcagcca gcgcaatcgg agccatcaac accgccgtcg cggtattaga gataaatagc
     2401 ccaataaccg ccgacaagac aaacaaacac cccagcatca tatgtggccc gtaaccaccg
     2461 ccaatgtcca tcagcccttt caccgccagc gcgacacctc ccgttttctg taatgccaca
     2521 gcaaacggca tcatcccaac gatcaaaata atgctcggcc agtgaatgga tttataggcg
     2581 ctttcagcat ctatacagcg gaatttcccc atcagcaggc aggcgatgat agcggcaaca
     2641 ggattaggaa tttcatctgt cagcattaac gccaccatta gcaccagaca gaaaatggca
     2701 tggggtgcct ggctgtgcgc gggcgatgct tcactcacct cttccggtaa gttcagcgct
     2761 acgaagtcgc ggccctgttt ggccagcata ccgatcagtt tccagttacc cacaaccagg
     2821 atgatatcgc ccagcagcag aggctcatcc gccagcgaac cttccagcgc cacgccattg
     2881 cgctttagcc ccaccacatt cagtccgtag cgggtacgaa aaccaatttc gcgcaccgat
     2941 ttaccaatca gttctgactc aggaattaat gaaatctctg ccatgcccac atcaagggcc
     3001 tggtcagaaa aatactcgcc gcgcagtacc atcggctcca gcaattgctc actacaaaat
     3061 tgccggagat cgacatcagc cgcagacata tcaataagca aaacgtcacg cgcgcgaaat
     3121 tcagaaaccc cattaacgtt cacgataacg cgacgaaaac gccgccagcg ttcaacaccg
     3181 atgacgttag cgccataacg ctcacgtaat ttgagatcat ccagccgttg acctatcatt
     3241 ggcgatccgg ggcgaatagc cagacgtcgc gcacgcccgg tcagtcgata ttcacggata
     3301 agatcgcgaa aggttcgacg cgtccagcct tcgcgctgcg gggtctgggt atcccctttc
     3361 agcatgaaac gcatcactaa catatacaaa atacccagca ccagcacgac caggccaata
     3421 ggtgttacgc taaagaaact gaagccgtga tagccttcac gcagcaattc actgttgact
     3481 accaggttcg gcggcgtcgc caccagcgtc atcatgccgc taatcagccc ggcaaaactt
     3541 aacggcatca tcagacgtga cggcgaagtt tgcatacgca tggcaacgct taacaccacg
     3601 ggaataaaga tagcgacaac gccggttgaa ctcataaacg cgccaagccc cgcgacggtc
     3661 agcatcaaca aaaccaacat tttgatttca ctgttgcccg ccactttgac cagccatgtt
     3721 cccattacgg tggcaacacc ggtacggacc aaaccatcgc caataataaa caaggcggca
     3781 atcaggacaa cgttaggatc agaaaagccg gaaaatactt cggggaccgt cagcgttccg
     3841 cttaatgcaa acgcgacaat aacaaacaaa gcgaccgcat ccatacgcac tctgcccgtc
     3901 gcaaacaaga cgatggcaac ggccagtaat gaaagaaccc aaatcaattc accgttcaca
     3961 acttatcctt gttaattgag ggggatgact tgattctgcc ataaaaaagc cccgacgaga
     4021 cggggctaaa tcatgatcag gtgtttcact gaataataac atcgccattt ggctgtttgg
     4081 tcacagtaat ttgctccaga ctgtggagga ccaaatcgac ctcattcagg cgcggggtat
     4141 ctgccggagc gttaaccgca atgacatggc aacccgccgc caggccagaa agcacgccag
     4201 cgggagcatc ttccaccacc acacactcct gcggcgcaag ccccagcagc tgcgcaccta
     4261 acagatacgc atcaggttct ggttttccgc gcttcactcg ctcagcggtt acaaacacct
     4321 ctggtgcggg aagcccagct attttatggc gcgctcgcgc taccggcatg gagccagaag
     4381 tcacaatggc ccacggaata cctgctttat tcaaatgatt gagtaaggcg attgcccccg
     4441 gaagcgcggt aataccttcg gtttccgtgg cctcgatgtg ctccagacgc gtaaactcgg
     4501 cggcaatatc agcctcggat ttgcccgcca taaaatggcg caaagaggtg atcgcctgtt
     4561 taccgtgaat gaaagccagc acctcttccg gcgctaaccc atgacgtctg gcccagttgc
     4621 tccacgcccg ttctaccgca ggcagggaat ccaccagcgt tccatcaaga tcaaacagaa
     4681 aacctttgca ccgcacgcgg gcctcctcag gcgttgataa tttgattaat ttcgttggcg
     4741 ctcaaatggt actgacgcgg gcaggcatgc cacacattaa gcatgcgctg atatttttcc
     4801 cacattggcg tctgggcgtt aaaaccgtga gttccggcgt caaaatgggt atagcgccct
     4861 tccacattaa ccataaagcg gacataaccg aggtaacgtg cttcagtggc ggcgtcaaag
     4921 ccgaggaagg tgacacgacg ttcatcgatg gattgctggt cctgcaaatt agaccaggaa
     4981 acatgcaacg catgatacat ctccataatg tcgatgatag tgcggcaggt ttcttctttc
     5041 agctcgccaa actcgcgatc caattcacgc atctgtaatc cgtaaccacg ctcaataatt
     5101 gtttgcaggc gacggtaacg ttcagcattt gccggatcga gcatagtcat catcttgtac
     5161 tgattagaca aaataagacg ttgcgcgttg gtcatttcca ttgttgactc ctgtatcact
     5221 atactacggt gaaaaaaaag aaggctgagt atgccttctt ttatatgcgt aatcaggggt
     5281 caattacaaa tcatcaagga aagttttatc cagttgtttg aaggcgcgct taagcgtgtc
     5341 agctaatgcc tggtaatcag gcttgccttc aacgggtgcc aacacctgtc cagactcctg
     5401 caatttaccg cgaacttcat aaaaccagtt aaggattgca gggggtaatg gcgttacaga
     5461 acgcttgccc agccaccaca atccctgcat gggtaaactt aaggcgaaca gcgcagtggc
     5521 aactgccggc ccaagctgac cgcccagggc aatctgccag cagagagtaa atacggcgat
     5581 cggcggcata aaacggatcg cataacgcgt catcttgata acgcgatttt cgacaaagac
     5641 cggggcaagg cgtttttcca gcggccacgt ctttgagtaa tgctgtcccc ggcgaaacaa
     5701 gctaaaaaaa ttaacagaac gattatccgg cgttgacatg cttcacctca acttcacata
     5761 taaagattca aaaatttgtg caaattcaca actcagcggg acaacgttca aaacattttg
     5821 tcttccatac ccactatcag gtatccttta gcagcctgaa ggcctaagta gtacatattc
     5881 attgagtcgt caaattcata tacattatgc cattggctga aaattacgca aaatggcata
     5941 gactcaagat atttcttcca tcatgcaaaa aaaatttgca gtgcatgatg ttaatcataa
     6001 atgtcggtgt catcatgcgc tacgctctat ggctccctga cgttttttta gccacgtatc
     6061 aattataggt acttccatgt cgagtaagtt agtactggtt ctgaactgcg gtagttcttc
     6121 actgaaattt gccatcatcg atgcagtaaa tggtgaagag tacctttctg gtttagccga
     6181 atgtttccac ctgcccgaag cacgtatcaa atggaaaatg gacggcaata aacaggaagc
     6241 ggctttaggt gcaggcgccg ctcacagcga agcgctcaac tttatcgtta atactattct
     6301 ggcacaaaaa ccagaactgt ctgcgcagct gactgctatc ggtcaccgta tcgtacacgg
     6361 cggcgaaaag tataccagct ccgtagtgat cgatgagtct gttattcagg gtatcaaaga
     6421 tgcagcttct tttgcaccgc tgcacaaccc ggctcacctg atcggtatcg aagaagctct
     6481 gaaatctttc ccacagctga aagacaaaaa cgttgctgta tttgacaccg cgttccacca
     6541 gactatgccg gaagagtctt acctctacgc cctgccgtac aacctgtaca aagagcacgg
     6601 catccgtcgt tacggcgcgc acggcaccag ccacttctat gtaacccagg aagcggcaaa
     6661 aatgctgaac aaaccggtag aagaactgaa catcatcacc tgccacctgg gcaacggtgg
     6721 ttccgtttct gctatccgca acggtaaatg cgttgacacc tctatgggcc tgaccccgct
     6781 ggaaggtctg gtcatgggta cccgttctgg tgatatcgat ccggcgatca tcttccacct
     6841 gcacgacacc ctgggcatga gcgttgacgc aatcaacaaa ctgctgacca aagagtctgg
     6901 cctgctgggt ctgaccgaag tgaccagcga ctgccgctat gttgaagaca actacgcgac
     6961 gaaagaagac gcgaagcgcg caatggacgt ttactgccac cgcctggcga aatacatcgg
     7021 tgcctacact gcgctgatgg atggtcgtct ggacgctgtt gtattcactg gtggtatcgg
     7081 tgaaaatgcc gcgatggttc gtgaactgtc tctgggcaaa ctgggcgtgc tgggctttga
     7141 agttgatcat gaacgcaacc tggctgcacg tttcggcaaa tctggtttca tcaacaaaga
     7201 aggtacccgt cctgcggtgg ttatcccaac caacgaagaa ctggttatcg cgcaagacgc
     7261 gagccgcctg actgcctgat ttcacaccgc cagctcagct ggcggtgctg ttttgtaacc
     7321 cgccaaatcg gcggtaacga aagaggataa accgtgtccc gtattattat gctgatccct
     7381 accggaacca gcgtcggtct gaccagcgtc agccttggcg tgatccgtgc aatggaacgc
     7441 aaaggcgttc gtctgagcgt tttcaaacct atcgctcagc cgcgtaccgg tggcgatgcg
     7501 cccgatcaga ctacgactat cgtgcgtgcg aactcttcca ccacgacggc cgctgaaccg
     7561 ctgaaaatga gctacgttga aggtctgctt tccagcaatc agaaagatgt gctgatggaa
     7621 gagatcgtcg caaactacca cgctaacacc aaagacgctg aagtcgttct ggttgaaggt
     7681 ctggtcccga cacgtaagca ccagtttgcc cagtctctga actacgaaat cgctaaaacg
     7741 ctgaatgcgg aaatcgtctt cgttatgtct cagggcactg acaccccgga acagctgaaa
     7801 gagcgtatcg aactgacccg caacagcttc ggcggtgcca aaaacaccaa catcaccggc
     7861 gttatcgtca acaaactgaa cgcaccggtt gatgaacagg gtcgtactcg cccagatctg
     7921 tccgagattt tcgacgactc ttccaaagct aaagtaaaca atgttgatcc ggcgaagctg
     7981 caagaatcca gcccgctgcc ggttctcggc gctgtgccgt ggagctttga cctgatcgcg
     8041 actcgtgcga tcgatatggc tcgccacctg aatgcgacca tcatcaacga aggcgacatc
     8101 aatactcgcc gcgttaaatc cgtcactttc tgcgcacgca gcattccgca catgctggag
     8161 cacttccgtg ccggttctct gctggtgact tccgcagacc gtcctgacgt gctggtggcc
     8221 gcttgcctgg cagccatgaa cggcgtagaa atcggtgccc tgctgctgac tggcggctac
     8281 gaaatggacg cgcgcatttc taaactgtgc gaacgtgctt tcgctaccgg cctgccggta
     8341 tttatggtga acaccaacac ctggcagacc tctctgagcc tgcagagctt caacctggaa
     8401 gttccggttg acgatcacga gcgtatcgag aaagttcagg aatacgttgc taactacatc
     8461 aacgctgact ggatcgaatc tctgactgcc acttctgagc gcagccgtcg tctgtctccg
     8521 cctgcgttcc gttatcagct gactgaactt gcgcgcaaag cgggcaaacg tatcgtactg
     8581 ccggaaggtg acgaaccgcg taccgttaaa gcagccgcta tctgtgctga acgtggtatc
     8641 gcaacttgcg tactgctggg taatccggca gagatcaacc gtgttgcagc gtctcagggt
     8701 gtagaactgg gtgcagggat tgaaatcgtt gatccagaag tggttcgcga aagctatgtt
     8761 ggtcgtctgg tcgaactgcg taagaacaaa ggcatgaccg aaaccgttgc ccgcgaacag
     8821 ctggaagaca acgtggtgct cggtacgctg atgctggaac aggatgaagt tgatggtctg
     8881 gtttccggtg ctgttcacac taccgcaaac accatccgtc cgccgctgca gctgatcaaa
     8941 actgcaccgg gcagctccct ggtatcttcc gtgttcttca tgctgctgcc ggaacaggtt
     9001 tacgtttacg gtgactgtgc gatcaacccg gatccgaccg ctgaacagct ggcagaaatc
     9061 gcgattcagt ccgctgattc cgctgcggcc ttcggtatcg aaccgcgcgt tgctatgctc
     9121 tcctactcca ccggtacttc tggtgcaggt agcgacgtag aaaaagttcg cgaagcaact
     9181 cgtctggcgc aggaaaaacg tcctgacctg atgatcgacg gtccgctgca gtacgacgct
     9241 gcggtaatgg ctgacgttgc gaaatccaaa gcgccgaact ctccggttgc aggtcgcgct
     9301 accgtgttca tcttcccgga tctgaacacc ggtaacacca cctacaaagc ggtacagcgt
     9361 tctgccgacc tgatctccat cgggccgatg ctgcagggta tgcgcaagcc ggttaacgac
     9421 ctgtcccgtg gcgcactggt tgacgatatc gtctacacca tcgcgctgac tgcgattcag
     9481 tctgcacagc agcagtaatc tcgtcatcat ccgcagcttt gcgctgcgga tatctgaacc
     9541 ggaaataatc actatttccg gttttttatt ctcttaatct gcattaatcc tttctgatta
     9601 tcttgcttaa ctgcgctgca tcaatgaatt gcgccatttc actttgcata cttaccactt
     9661 tgttttgtgc aagggaatat ttgcgctatg tccgcaacca ctgaatccaa accaacaaga
     9721 agatgggcaa tgcccgatac gttggtgatt atcttttttg ttgctatttt aaccagcctt
     9781 gccacctggg tagttccggt ggggatgttt gacagtcagg aagtgcagta tcaggttgat
     9841 ggtcaaacaa aaacacgcaa agtcgtagat ccacactcat ttcgcattct gactaacgaa
     9901 gcaggcgaac ctgagtatca ccgcgtacag ctgttcacga cgggcgatga acgcccgggc
     9961 ctgatgaact tcccgtttga aggattaacc tcaggatcga aatacgggac agctgttggc
    10021 atcatcatgt ttatgctggt gattggcggc gcgtttggca ttgtgatgcg tacaggaacc
    10081 attgataacg gtatcctggc gcttattcgc catacccgcg ggaatgaaat tctctttatt
    10141 cctgcgctgt ttattctgtt ttcacttggc ggcgcggtat ttggtatggg agaagaggcc
    10201 gtcgcctttg ccattatcat cgcaccgcta atggtccggc tgggctatga cagtattacc
    10261 accgtcctgg tgacctatat tgccacgcaa atcggttttg ccagttcgtg gatgaacccg
    10321 ttttgtgtgg tcgttgctca ggggattgcc ggcgttccgg tgctttctgg ctccgggttg
    10381 cgcatcgtgg tgtgggttat cgccactctg attggcctga tctttaccat ggtgtacgcc
    10441 tcacgagtga aaaagaatcc tcttctgtca cgcgtgcatg agtccgaccg cttctttcgt
    10501 gaaaagcagg ctgatgttga acaacgtccg tttacctttg gtgactggct ggtattgatt
    10561 gtcctgaccg ccgtaatggt ctgggtgatt tggggcgtga tcgttaatgc ctggtttatt
    10621 ccagaaattg ccagccagtt cttcaccatg ggtctggtga ttggcatcat cggcgttgtt
    10681 ttccgcctta acggcatgac ggttaatacc atggcttcat cctttaccga aggggcgcga
    10741 atgatgatcg cccctgccct gctggtgggt ttcgccaaag ggattttgct gctggtcggt
    10801 aatggtgaag caggtgatgc cagcgtgtta aataccatcc tcaacagcat tgccaatgcc
    10861 attagcggtc tggacaacgc tgtcgcggcc tggtttatgt tgctcttcca ggcggtattt
    10921 aatttctttg tgacatccgg ttctggtcag gcggcgttaa ccatgccgtt actggcaccg
    10981 cttggcgatc tggtcggtgt taaccgtcag gttaccgtgc tggcattcca gtttggtgat
    11041 ggcttcagcc acatcattta cccaacctca gcttcgttaa tggcgacgct cggtgtttgc
    11101 cgggtggact tccgtaactg gctgaaggtg ggcgcgacac tgcttggact gctgtttatt
    11161 atgtccagcg tcgtggtgat cggcgctcag ttgatgggct accactaaaa atgttaagag
    11221 ccgcaattgc ggctcttttt tcattctgcc gtttcagtct ctacggcttc atttttggca
    11281 ttgcgcttca tccacaacgc cagcgctttc agcgagtctg gagtgaactc atcgcagcgt
    11341 gcggtgattt cttccggcgt cagccagcaa acttcactga cttcatcttc ctgtagggcg
    11401 aagggaccgt gagagacgca gctgaacaat gcgccccaga cacggcaatt tttatcttcg
    11461 aaatagaact gcccgtgctc agcaaagggg acaccggcaa tgcccaactc ttcttccgct
    11521 tcgcgacgcg cggattccag cagttgctca tcggcctgga ccacaccgcc tgcggtcgca
    11581 tctaacatgc cgggtaaaaa gtcttttgtc tcggtacgac gctggaccag aattttgccc
    11641 atgccatcat gcacgacgat gtaagttgca cgatgacgca gacactgtgc ccgcatttgt
    11701 tcccggctgg cttgtgcaat gacttcgttc tcttcattga caatatccac ccattcagta
    11761 cttgccaaac gacgctgttc cattatcagg aaaccttctt tttctggcgc tcttacggcg
    11821 cattttgagt tgtgggtaaa ttacggatta atcgcgacct gcgcaatgat actttgatca
    11881 ttgagtgcga ttacactaag tacgtcatta tccagcatgc cataactcgc cggattaccg
    11941 cctttcggaa tactcaccga gccggggttg aagtggaaaa tctccccccg ctgttctgcc
    12001 actggcagat gggtatggcc gtacaccagc acatcgttct ggtttaaagc aggcagattt
    12061 tccgggccaa aaagatgacc atgcgtcaaa aacagacgtt gtttttccag taatacctgt
    12121 tgccacggcg cggttatcgg gaaatgcagc agcatttgat ccacttcgct gtcgcagttg
    12181 ccgcgcacag cgataacctt atgtgccact tcattaagcc gttcagcgac tttggctggc
    12241 gcgtaaccct ccggtaaagc attacgcggg ccgtgattca acacgtcgcc aaggatcacc
    12301 agccactggg caccgctttg ggcaaacaac tccagaacac gttccgtcgc cggtaacgac
    12361 ccatgaatgt ccgatgcaaa catcagtttc atcactcact cctcgtcgaa aaaacgtaag
    12421 ctatgatact ggattctgtc gttgctatca gccagattgc ttcgccgaaa gtgcaataaa
    12481 acgctggaca gacgctcgct gccactggaa tgtcgcataa tccaccaggc gttctggcac
    12541 ctcatcgcca tgtagtacca ggcggttaat cattagcgcc agatcagtat cagcaatgca
    12601 ccattcacca aataaattcg gctgacccag tactaacaaa tgttctgcca tcgcaaacag
    12661 tttctctgca ctggcttttc cctcggccgt tagtggcgct tttttcgccc ccgcaaagac
    12721 aacatccgtc ggacgctctt cgcggatggg catcagatcg ctgcgcagcc aggcctgaat
    12781 ctgtcgtgca cgcgcacgat tttctaaatc aagcggataa atacgttccc aggtcggtgg
    12841 cgcaaatcga tcttccagat actccgcaat ggcagaagat tcactcaact caaaatcatc
    12901 gatttgtaat aacggcacac ggcgtgtctg accgtaacct tgccacgtcg gctgcaaatg
    12961 ttcaccgctg tcgaggtcga tggtcttgat atgaaacgac aggccttttt cctgcaacgc
    13021 cacccaggcg gataacacat aaggggagaa aaagtgggca tctgaccaaa gcgtgatagc
    13081 gggtttactc attatgtcct cggcaataaa gggggctggc tatcaaacta tagagccttt
    13141 ttgtcactgt cacgtgacga atactcacgt aagtcgccgc gctttctata cttgtggtgt
    13201 tcagatgatt aaacaaccgg agctgcaatg atcgatctct atttcgcccc gacacccaat
    13261 ggtcacaaaa ttacgctgtt tctcgaagaa gcagagcttg attatcgctt gattaaggta
    13321 gacctgggga aaggcggtca gtttcgtccg gaatttttgc gcatttcgcc caacaacaaa
    13381 attccggcaa ttgttgatca ttctcctgcc gatggcggcg aaccgttaag cctctttgag
    13441 tctggtgcca ttttgttgta tctggctgag aaaacaggac tctttttgag tcatgaaacg
    13501 cgtgagcgcg ccgccacatt acagtggtta ttctggcagg taggcggact ggggccgatg
    13561 cttgggcaaa atcatcattt taatcacgca gccccccaaa ccattcctta cgctattgaa
    13621 cgttatcagg ttgaaactca gcgtctttac catgtactga acaagcggct ggaaaactcg
    13681 ccctggctgg gaggcgagaa ctacagcatt gcggatattg cctgctggcc gtgggttaat
    13741 gcctggactc gccagcgaat tgacctcgca atgtatccgg cagtcaagaa ctggcatgag
    13801 cggatccgtt cgcgccctgc caccgggcag gcactgctaa aagcacaact cggtgatgag
    13861 cgttcggata gttaacagaa acaggttctc gtgtattatt tcatcctaag taaaacaacg
    13921 gagaacctgc aatggcacaa cctgccgcta ttattcgtat aaagaacctt cgtttgcgta
    13981 cgtttatcgg aattaaggaa gaagaaatta acaaccgtca ggatattgtt atcaatgtga
    14041 cgatccacta ccccgccgat aaagcgcgca ctagcgaaga tatcaacgat gcgctgaatt
    14101 atcgcaccgt aacgaaaaac attattcagc atgtagagaa taaccgtttc tctttgctgg
    14161 aaaaattaac tcaggatgtg ctcgatatcg cacgtgaaca tcactgggtg acgtatgctg
    14221 aagtggagat cgataaactg cacgcgctgc gctacgccga ttcggtatcc atgaccttaa
    14281 gctggcagcg ttaaccgcca tatcgggagg ctgcatgaac atagtgatca ccggagggac
    14341 gggattaatt ggtcgccatt tgattccgcg tttgctggag ctgggccatc aaatcacggt
    14401 agtgacgcgt aacccacaga aagccagttc cgttctcggc cctcgggtga cgctatggca
    14461 agggctagcc gatcaaagca acctcaacgg cgttgatgcg gtaatcaacc tggccggaga
    14521 accgattgct gataaacgct ggactcacga gcaaaaagag cgtctctgcc aaagccgctg
    14581 gaatatcacg caaaaactgg tcgatttgat taatgccagc gacacgccgc cgtcggtact
    14641 catttccggc tcggcaacgg gctattatgg cgacttaggt gaagtggtgg ttaccgaaga
    14701 ggaaccgccg cataacgaat ttactcataa actctgcgcc cgctgggaag agattgcctg
    14761 ccgggcgcaa agtgacaaaa cgcgagtgtg cctgctgcga accggcgtgg tactggcacc
    14821 ggacggcggt attctcggta aaatgctgcc accgtttcgt cttggcctgg gcgggccgat
    14881 tggttccggt cggcagtatc tggcctggat tcatattgat gatatggtca acggcattct
    14941 ctggctgctg gataacgacc tgcgcgggcc atttaatatg gtttcgccct acccggtacg
    15001 caatgaacaa tttgcccatg cgctcggtca agcgctgcat cgcccggcca ttttgcgcgt
    15061 ccccgcaacc gccattcggc tgttaatggg cgaatcttca atactggtat taggtggaca
    15121 acgcgcgctg cctaaacggc tggaagaagc gggttttgcg tttcgctggt acgatttaga
    15181 agaggcgctg gcggatgtcg ttcgctgatg tggtttacag caaacatccg ccagttaact
    15241 cccggtgtta caggattagt ggctttgcgc gataagatcg tctggtgaaa gtcgggtcac
    15301 catcataact aactctctgt ctaaacctct atccagcatc tcctgagcaa tacgcagggc
    15361 ttcttcgtgt ttgccctgca ttgcgccttc ttcacgtaat ctgtcagcaa tggtcatcag
    15421 tttctccttt tcttgtggtg cgcgttctgt tatctcacca ataaatgcac ggaaacgctg
    15481 ggcatcccct gtttgtaata cgtaattaaa cagggctttt agctgtctgt cattagtgtt
    15541 ccctgtaact agcagcgaaa caatttggtc gactaatccc aacagatcgc gctgacgaat
    15601 atgtttctga attaactcca acagcgccat tttgcggtgt tgcataatct cgtcatccgg
    15661 caccacggta atatccacca acggaaacgc tgatgaatat attttgcggg ctatagcagg
    15721 ctcggcaaat tcatcaagcc agcagagtga ataaggataa ggacttctgc aaccatgata
    15781 aaacagcatc gggatcacca atggaagctc tttatagccc gcatcaagat ggttttgcat
    15841 tgccgcaatg gaataacgca tcatgcgaaa agccattaat tcttccggct tactttggtg
    15901 ctctatcact acataaatat aacccactcc ctcctgcgtt ttcacagacc acaagaggtc
    15961 ggaataatat tgccgcaggt cgtcatcaat aaaactgttt ggttccagtt taagcgtcgt
    16021 taaatcacac agtttgcgca gcggcgcggg aagatgaata tcaataaaat cccgcgcggt
    16081 gtctggatgg cgtaaaaaag atttaaatac cgcatcatgc ggcgtggaag ttgtcgatat
    16141 tgtcatggcg ttccgtcacc cctcaaattt aagatgacgc gacaataacc acgattaaca
    16201 cccaatgcag caggcagttt ttatctttgg acagcgaatt cagaagtatt tttagcggac
    16261 tgcaactttt acatttcagc ggaagctgac tcacaaattc cccatgaaaa tactccggca
    16321 gcgttagcca ccggagagga aattatttca gcgatccctt aaggaactgt tgcagacgag
    16381 ggctttgcgg gttgccaaat aactgctccg gcgcgccctc ttcttctatt tttccctgat
    16441 ggaggaaaat gacgtgagta gaaacatggc gagcaaagcc catttcgtga gtcactacca
    16501 ccatggtttt cccctcttct gccagttgct gcataatacg taacacttcg cctaccagtt
    16561 caggatcgag cgccgaggta ggttcatcaa acagtaaaac ttccggttcc atcgccagcg
    16621 cccgcgcgat agaaacacgc tgttgctgac cgccggaaag atgcaccgga tatttcccct
    16681 gcgcacgttc gtctatcccg actttcgcca gatacttcac cgcccgctcg cgcgcttcct
    16741 gcttgctcag gcccaacacc tgaatcggcg cttccatgac gttttccagc accgtcatat
    16801 ggctccagag attgaagtgc tggaatacca tcgtcaggcg tgtgcgcagt aagcgcagtt
    16861 gatttttatc ggcgactttg agttgaccat ctttgtcgcg caccagattg atcgtctggc
    16921 cattgaccac gatcgaccct tcactcggtt tttcgaggaa gttaatgcag cgcagaaagg
    16981 tacttttccc cgatcccgac gatccgatga tgcttattac atctccggca ttcgcttgca
    17041 gtgagacccc tttcagcact tcatgttcgc cgtagcgttt gtgcaaatcg ataacgttta
    17101 atttattctc ggacatcgta ttctcagtgc gttgaagaag gtttcacatg ctgcaaccag
    17161 cgtttttccg ctctgcgaaa gaggctgatc aggacataag agatgattaa atagagcacc
    17221 gcggcaatgc cgaaggcggt aaaaggttga tacgtggcgg cgttaatatc gcgggctatt
    17281 ttcagcagat ccggcacagt ggccgtaaac gccagcgcag tagagtgcag catcaggatc
    17341 acttcgttgc tgtatgccgg taacgcaata cgcagcgcag aaggcaaaat aatgcaacga
    17401 tacattttaa aagtcgagaa gccataggct ctggcggctt caatttcccc atgcggaacc
    17461 gaacggattg ccccagcaaa aatctcggta gtgtaagcgc aggtgttaag cgtcagcgcc
    17521 agcacggtac agttcaggcc actgcggaag aaagcgttaa ggaattcggt tcccttaaca
    17581 atctcaagcg tgtacatgcc ggaatagaac accagcaact gaacatacag cggcgtaccg
    17641 cgaaaaatat aggtaaataa ccagattgga aactggatgt atttattact ggagacacga
    17701 ccaatcgcca gaaacagcgc caggactccg cctatcacta ccgacaaaat aagcagccac
    17761 agagtgatcg ccacaccagt aaagcgataa ccgtcggtcc acagcagcgg tttccagtat
    17821 tcatgtaaga tttcgatcac aggtcagccc tcttcacacc cacggagtag cggcgctcaa
    17881 ggaacagcag cacaccattg gaaacagtgg tgaaaaccag gtaaatcacg ccacagacga
    17941 tggcgaaata gaacggttcc caggtacttt tgcctgccag ttgggtggct ttgaccacat
    18001 cttccaggcc gagtaacgaa accagtgcgg tagatttgag gatcacctgc cagttgttgc
    18061 caatgcctgg cagggcgtaa cgcatcatcg ccggaaacat gatccgccga aacacttgcc
    18121 cacgagtaaa accgaacgcc gtcgccgcct ctatatgccc tttcggcact gccataaacg
    18181 cgccacggaa cgtttcggta aaataagcac cgtagataaa accgagagta ataataccag
    18241 cgaccatcgg atcgatatca atctgcccga cgcccatcgc ctccgtcacc gtgtttagtg
    18301 caatctgcaa accgtagaaa atcagcagca tcaacaccaa gtccggcacg cctcgaatca
    18361 gcgtggtgta cccttcgaaa atcagccccg aaagccgatt ttgcgagagc ttaccgccag
    18421 cgccaattaa accgatgatt acagcgagca ctacagaact gatagccagc tccagcgtga
    18481 cgagcgcacc ctgtaaaata acacctgaaa acccatacaa catgctgcct gtcctgtcgt
    18541 gtgtggtgaa ttaccgcctc ttgtctccct ccggttaccc gaagggagcg ggctttcttt
    18601 caccgatggc gattaaccgc cataaacatc aaaatcgaag tactttttcg ctaatttctc
    18661 gtaagtaccg tcagcgcgca tttcggcaaa ggctttgttc agtgcctcgc gcagttcgtt
    18721 atcttcttta cgcaggccca tgccggtccc tacgccaaac agtttttcat ctttaacaga
    18781 cgggccaccg aatttgtaat ctttaccgac aggttgtttg aggaaacctt cgctggcagc
    18841 gacctcatcc tggaacgcgg catcaatacg tccggcagtc aggtcagaat aaatgttgtc
    18901 ctgcccctga tacgagacga tttcaatgcc ttttggtgcc caatgttcat taccgaacgt
    18961 ctcctgggtg gtgccctgca atacgcctac ccgtttgcct ttcagcgact cgactgtcgg
    19021 ctgaatgtcg gaatttttcg ccaccaccaa acgagaatcg gcagcgtaca gtttgtcggt
    19081 gaaggctatt tcttgctgac gtttttccgt aatggaaagc gatgacatga tggcgtcaat
    19141 cttcttcgct tttaaggacg ggattaacgc atccagcgga ttttcgacaa acgtacattg
    19201 cgtattgatg cgtttgcata attcctttgc cagatcgata tcgaagccaa ccagttcgcc
    19261 ttgtgaattt tttgattcaa atggcgcata ggtcgggtcg gtaccgatgc ggatgttttg
    19321 cggaatcgca gcaaacgccg cagttgcgct ggagaaggcc agaaccagag agagcgatag
    19381 caccagtttt ttcataatgt cctcaactga cagtcttttt atagggaata tttacaggtt
    19441 tgttacactt atcgtgccat aaaattgacc atcaaggcaa aatatcccgc cccgttagac
    19501 gtattatttt tcattaaaaa tgcttaagta tgcatttcaa tgcaccacga tagtgcaaca
    19561 aacctggcgt gcaccatgat gatcattcca tcaggtacag cttcccagcg acgtatcagt
    19621 caccgtagac attaaagtcg aaatactttt tcgccatctt gtcgtaggtg ccgtcctgac
    19681 gcagctcgcc aagcgcctta ttgaaggcag ccgtcagttc agcatcatct ttacgtagcc
    19741 ctacaccggt gccatcaccg aagtattttt tgtcttttac tgatgagcca gcaaaggcga
    19801 aatctttacc agcaggttgc ttgaggaatc cttcgctggc agcaacttca tcttgtaacg
    19861 cagcatccag acgtcctgca gccagatcgg aatagaccaa atcctggttg gcataggcca
    19921 ccacatccac gcctttgcta cgccaggtct cgttagcgta agcttcctgg gttgatccct
    19981 gcagcacacc aacatgttta cctttcagtg aatccagcgt tggctgaatc ggtgaacctt
    20041 tggcagcaat caaacgagaa tctgcggcgt acagcttgtc ggagaaggca atctcctgct
    20101 gacgtttatc ggtaatggaa agcgacgaaa taatggcgtc gatttttttc gctttcagtg
    20161 aggggatcag cgcgtcaaag tcactggcaa cccaggtaca tttcacctgc atccgtttgc
    20221 acatctcgtt accgagatcg atatcaaagc caacaaaatc acctttagca tctttcgacg
    20281 agaacggcgc ataggtggta tcggttccga tacgtaccgt ctccggtagc gccgcatagc
    20341 tggaagccgc tgcggagaga ccgactaaca aagacagagc gagaatcgac ttcttcatac
    20401 ataaccctca agtgaaaatg gcttttttat gttgtgtatt gtgttgtgtg tttgcaggct
    20461 ctttcatgca ggtcttatgc catcttgacg acaacagtaa cattcaacgt taaatatgtt
    20521 aataagacgt tgcattattg tcctgaagtt gaagatagca ggtatagcgg ttgaatcgca
    20581 gcgtttcgat tgaatggcag aaacaaaatg tcgaggattt gatcgcggtt gcaaaattgc
    20641 cctgaaacag ggcaacagcg gagttatgcg ccctgccagc gggcaaagag atcttcagga
    20701 agggttattg caaactggtc aagaacacga ttaaccgtct gatttatcac atcatcaagg
    20761 gattgcgggc gatgataaaa cgccggaacg ggaggcataa tcaccgcacc gatttctgcc
    20821 gcctgagtca ttaaacgcag atggcctaag tgcaatggtg tttcacgcac gcagagcacc
    20881 aacgggcgac gctctttcag caccacatct gccgcacggg tcagtaagcc atcagtatag
    20941 ctatggacaa tgccggaaag ggttttgatt gaacagggta aaatcaccat ccccagcgtc
    21001 tggaaagaac cggaagagat gctggcggaa atatcgcgcg catcgtgcgt gacatcagct
    21061 aatgcctgca cttcgcgcag ggaaaaatcc gtttcgaggg ataaggtctg gcgcgccgcc
    21121 tggctcatca ccagatgcgt ttcgatatct gtgacatcgc gcagaacctg taataagcgc
    21181 acgccataaa tcgcgccgct ggcaccgctg atgcctacaa tgagtcgttt cataaatagt
    21241 tgccctgtca gacttgcagg cagactttgc agtatttcgg cgggagttgc aagtcagggt
    21301 gccagaccgg caccctcagc gaaggcatca tccttcgtta tgcatttcga gattttccac
    21361 ttcgttctga cgttgcactg ctttggcgtc atcattacgt aacgtatcga ggaaatcgag
    21421 gtagccctga tcaacatctt tggtgacgta gacgccgttg aacaccgagc attcaaactg
    21481 ctggatatcc ggattttcag cgcgaacggc gtcgatcaga tcgttcagat cctggaaaat
    21541 caacccgtca gcaccgatga tctggcgaat ttcatcaact tcgcgaccgt gagcgatcag
    21601 ttccgtggcg ctcggcatat caataccata aacgttcggg aagcgaattt ccggtgccgc
    21661 agaagcgagg tacactttct tcgctccggc ttcgcgtgcc atctcgataa tctgctcaga
    21721 agtggtgcca cggacgatgg agtcgtcgac cagcaggacg tttttatcgc ggaactcggc
    21781 gcggttggca ttcagtttac ggcgcacgga cttacgacgc agctgctggc ccggcatgat
    21841 aaaggtgcgg ccaacatagc ggtttttaac gaagccctgg cggtacggtt tgcccagaat
    21901 acgagcaatt tccagcgcga tatcacacga ggtttctggg atcgggatca ccacgtcgat
    21961 atccagatct tcccattcgc gggcaatttt ctcgcccagt ttcgtgccca tattcacacg
    22021 cgcgctgtaa acggaaattt tgtcgatgaa cgagtccggg cgagcaaagt atacatactc
    22081 aaacaggcac ggattgctga ccggattgtc agcacattga cgggtaaaca actgcccttc
    22141 ttcagtgatg taaatcgctt cgcccggcgc gacgtcacgc aggaaatcaa agcccagcgt
    22201 atcgagcgct acgctttcgg aagcgaccat atattctgta cggttctcgt caatatcacg
    22261 ttttcccagt accagcggac gaatcccgtt tggatcgcgg aaagcaacca taccgtggcc
    22321 gataatcatc gccacacagg catatgcgcc gcggattaag cggtttgtgg cagcaatggc
    22381 agcgaaaata ttgtcggctt ccagcgggta gtggcggaag ttgtccagct cgctagcgaa
    22441 gatattaagc agaatttccg agtcggaagt ggtgttgatg tggcggcgtt tttcttcaaa
    22501 cagtttttta cgcaactcgt gagcgttggt cagattgccg ttgtgggcaa gcgtaatgcc
    22561 atacggagag ttaacgtaaa acggctgcgc ttcagaggcg ctggagctgc cagccgtagg
    22621 gtaacgcaca tgaccaatgc ccatattgcc ctgcaaacgc tgcatatggc gagcttcaaa
    22681 tacatcgctc accagcccgt tcgctttacg caaacggaag cagttattgg catctatggt
    22741 gatgatgccg gcggcatcct gaccgcgatg ctgaagcacc gttaaggcat cataaatcga
    22801 ctggttaacc ggcataacac cggcgatacc gacaataccg cacatacgtc tttttcctcg
    22861 ttaagccaca tctcagagca cttacgctct gggcaagaaa cttgacgagc tttgcagata
    22921 atcaaaaaac catctgatga taaaactgaa ttgcgggatc agctgtgatt tgctccagtc
    22981 ttcgcttttc gacaccccgg taaaggagtc gagaaagaag agaatggcgg caacaatcaa
    23041 cacaccgcgc aacgcaccga aacagacgcc cagcacccga tcggtgcctg acaaccccgt
    23101 tttctccacc aactggccta tcacgaagtt cacgatagca ccaacgatca gggtagcgat
    23161 aaacagtacc gcgatggcaa ttccatttcg aaccagttcg tcttcaaagc ccgtaaacca
    23221 gactgacagg taagtgtagt aatgactggc aacaaagaaa gcacaacccc atgtcaccag
    23281 cgataacgct tcacgaacaa agccgcggat caggctaacc agagaggaaa aagcaatcac
    23341 cgcgattatg gcgtaatcaa tccagaccat atgtgtccca cgattttacg ccctgtcatc
    23401 ctgttcgggg cgcattctaa cagaaaaaga aaacgtttgc gtagggattt ccttcccgcg
    23461 catcaataaa aatggcgctg aaaaaatatt caacgccatc gactttttat gcctttgcgg
    23521 catcgtgcaa tgcgtgtcgg atgcggcgta aacgccttat ccgacctacg gttctaacac
    23581 tgcccaggcc tgataagacg cgccagcgtc gcatcaggca agactgtatt aattcggcgt
    23641 atagcccatt accacgccac ttaagccaga aagttgcttc aactcaccca gcgaaccttt
    23701 cagcttatct ttcgaggcat ccggcccaac cagaatacgg gtaattttac cctgcactgg
    23761 cgtggatggc gacgtataaa cccgataacc ggcaccgcgc agcttaccga caatctcatt
    23821 cactttatcg gcatttttca gcgcacccag ttgcacaaca taggctttac ccgttggcgc
    23881 tgctttttct tccacgaccg gcttcggttc tggcttcggc gcaggtggtg cttcaacctt
    23941 cggtttaggc ggctccaccg gtttcgactt cggtggggcg accggtgcag gttccggttc
    24001 aaactcggtg ttattggctg caatagtggt cggatcgagc gacggtgctg cggcatcacc
    24061 tgcccgcacc tcttccgctg cgccttccgg cggctgcgtc ggcaacgcct gggtggcggc
    24121 tggcatcata tcaggctcat cgcgatcgcc cgctttcggc accagcggga tagccgcgaa
    24181 ctcatcctga taatgttttt tctgcccgtc cagcagccct ggaagtacaa tcacccccag
    24241 cgccaccagc acgatcgtgc ccactaaccg attctgaaac ttacttgcca ccgcttctcc
    24301 tcgcgtcaat cacttccatg acatgtgcga cagtgtggaa agaaccacac accagcacgg
    24361 tgtcttccgc tttagcgtcc gccattgcgg catcccatgc ctgcgcaacg ctatcaaatg
    24421 atttgccgtt acccaaatgc tcaagcagtt gttctgccgt ggcaccgcgc ggcccttcca
    24481 gtggcgcaca ataccagtca tcaaccacgc ttttcaacca ggccagagtt ccggcaatat
    24541 ctttatcatg tagcataccg ataaccgcca gcacgcgccc gtttttcggt agcgctttca
    24601 tacgcccggt gagatattcc gccgcatgtg gattatgcgc gacatcaaaa ataacgcgtg
    24661 gcgactcgct cacaatctgg aaacgtcccg gcaaaattgc gctggcaatc ccgtcgcgaa
    24721 tggcattttc actgacttcc agcccgctgg cacgcagtgc cgccagcgcc gttgcggcat
    24781 tcggttgcgg gacaagcggc aacggcagat tttccagcgt gccgtgagca tcgctaaacg
    24841 cccagtcatg atcggtgacg gaatagttcc actcaacgcc ccgacgttgt aacagtgcac
    24901 ctttttcctg tgccacatca gcaatggtag aaggcatttc cggctcaccg acaattgccg
    24961 gtttttcgct gcggaagatg cctgctttct cgcgaccaat actttcgcga tctggaccca
    25021 gccagtcggt atgatccagc gcaatactgg ttactaccgc gacatcggca tcgacaatat
    25081 tggttgcgtc cagacgaccg cccagcccta cttccagaat caccacgtca agttgtgcct
    25141 gcttgaacag ccacaacgcc gacagcgtac cgtactcgaa ataggtcagg gaaatatcac
    25201 cgcgtgccga ttcaatctcc gcaaaagagg cggtgtgggc cgattccggc aattcctggc
    25261 cctgcacacg tacgcgctcg gtataacgca ccagatgcgg tgaactgtag acgcccactt
    25321 tgtaccctgc cgccatcaga atcgactcca gcgtacggca ggtggtgcct ttgccattcg
    25381 tacccgcaac ggtaaacaca aatggcgctg gtttcagaac gccaagacgc gccgcgacct
    25441 ggctcacgcg ctcaaggccg agatcgatag ttttactgtg caggttttcc agataagaaa
    25501 gccacgaagc cagaggcgac gcggcttgag gagtgcgttt gataatcatg gtatccgctg
    25561 attcgttacg gtgagaatag caaaagggca gagccagtgg ccctgccctt atcagttatc
    25621 aggcctcagg ttcctgatcc ggtaccgggg gtaccactac gccttcacgc ggcgcttcag
    25681 gattcggcgc tggcagattc atcaacttcg ccagaatgct cgccagtttc aggcgcattt
    25741 ccggacgacg gacgatcatg tcgatcgcgc ctttctcgat caggaattca ctgcgctgga
    25801 atcctggcgg cagtttttcg cgaacggtct gttcgataac acgcggaccg gcaaagccga
    25861 ttaacgcttt cggttcagcg atgttgagat cgcccagcat ggcgaaactt gcagaaacac
    25921 cgcccatcgt cgggtcggtc agcacggaga tgtacggcaa gccgcgctcc tgcatttttg
    25981 ccagtgccgc agaggttttc gccatctgca tcagcgacat cagtgcttcc tgcatacgtg
    26041 cgccaccaga ggcggagaag cagatcagcg ggcagttatc ttccagcgcc tgctcaacgg
    26101 cacgcacgaa acgtgcaccc acaacagacc ccattgaacc gcccataaag gcgaactcga
    26161 atgccgcagc gacaaccggc attccataca gagtgccttt catcaccacc agcgcatctt
    26221 tttcgccagt ttctttctgc gcagatgcca gacggtcttt atacttcttg gagtcacgaa
    26281 acttcagcac atctttcggc tcaagctcgc tacccagctc cacaaggctt ccttcatcta
    26341 acaggctatg caggcgatta cgcgctgtca tacgcatgtg atggtcacac ttcggacaga
    26401 cctcaagatt acgttccagc tcagcgcggt ataaaacctg accgcagcta tcacacttag
    26461 tccacacccc ttcaggaatg ctcgccttgc gggtgggagt aatgttgctt ttaattcgtt
    26521 caatccagct cattagggac ctttctgtct gaacctggtt cgatgccagt tttatctttg
    26581 gggacgcata atgccatttt tgcccccaac agaccatgaa tgttgcacat taaaacataa
    26641 cagcccgaaa ctttggataa aaaagtggtc gaaccgcgga gttacttttt attttgcggc
    26701 gcgtgccgca gcgcgtttgt gacgaataaa ttcgaaaacc ccaggcaata ttgaaacaaa
    26761 aataatgcca acaatcatca gcttaaggtt gctctgaatg aaggggagcg tgccgaagaa
    26821 ataacccgcg taggtaaaaa tcagtaccca caacagtgcg ccgatcacgt tataagcggc
    26881 gaaatgacgg tacgacatgt ggcccattcc cgcaacaaac ggcgcgaacg ttctgacgat
    26941 cggtacaaaa cgggcgagaa taatcgtttt gccgccatgt ctctcgtaaa actgatgagt
    27001 cttatcaaga taactgcgac gaaagatttt cgaatccggg ttactgaaca gtttgtcacc
    27061 aaataaccgt ccaatggtat agttcactgc atcaccgaca atagcggcaa tcaacataag
    27121 caccaccatc atatggacat tgagatcgtt agtttccagc gacgccaatg caccagcaac
    27181 aaacagcagc gaatcaccag gcaggaatgg cgttaccacc agccccgttt cgcagaacag
    27241 aatcaaaaac agaatggcat aaacccagac gccgtactcc gcgaccagct ctgccagatg
    27301 cacgtcaatg tgcaggataa aatcgatgag gaaataaatc aggtccatat ttgcctatgc
    27361 cttgtactcg tcatctttca ggctgtaact acgttggcta cagcctgaaa tgttccgagc
    27421 attattctcg ttagtccgcc agaaatagcg ggcccattgg cggttttgga agatcatacc
    27481 ggtcagggta atccaccgcg accagataca gcccttccgc ttttgccgtt gctgccgccc
    27541 gcgttctgtc ctttgccgcc agcaactctg ctatccagct ctccggctgg ttgtgggcac
    27601 cgacttccat caggctgccg acaatattcc tgaccatatg atgtacaaag gcattcgctt
    27661 tgatatctac caccacataa ggaccgtgac gcatgacatt aatatgcata acgttgcgcc
    27721 acggggttcg ggactggcac tgcaccgcac ggaacgaggt gaaatcattc tcgcccagca
    27781 agcattgcgc agcccgatgc atccgttcag cgtccagcgg ttcgtaaaaa tgggttaccc
    27841 ctttactcag taccgccggg cgcagccgat gattgtagat gatgtagcga taacggcgag
    27901 ccgtggcgct aaatcgggca tgaaaatcat caggtacagc tttaacccaa cgcacggcga
    27961 tgtcaccagg taaattcgca tttacgccca aggtccacgc cgcgtctttg cgctgcgcgg
    28021 ttgtttcgaa atgcaccact tgcccggtac cgtgtacccc tgcgtcagta cgcccggcgc
    28081 agaagacggt gatgggttcg ttcgccacct gggagagcgc cttttccagc ttctcctgca
    28141 cactgcggac ttcattctgc cgttgccagc cgtaatactt actgccgtcg tactcaatgc
    28201 ccagcgcaat tttataaact ggcagttgtt gctggtcgga cattagtaca gatactcctg
    28261 caccagtttc tcggcgattt ttactgccat cagcgcgccg ccaaagcgaa cgttatcggc
    28321 caccgaccag aactggactt gctccggcat accgtagtca ttacgcacgc agccaacaga
    28381 aagatgcggc gtacccgaag catcccctac ctgagtcggg aattcgttct cttcagagag
    28441 cacaatatct tcgccctgag caaacgcatc acgcgcttct tctgccgcca gcggacgcag
    28501 tgcttcaaag ttgaccatct gggcatgacc gtagaatacc ggtgcctgga cgacgcttgc
    28561 cgaaatcatc agcccttcgt cctgcaggat tttgcgtact tcgtcaacga tacgacgttc
    28621 ttcacgcacg ctaccttcgc tatccggcaa caacggcagc atattgaacg ccagttgacg
    28681 accgaagaaa tcttcttcgt caatcggaat gccgttgagc aatttcgcac tctgccccgc
    28741 taacgcatcg accgcttttt tgccctgggc ggaggctgaa atcaggctgg taacactgat
    28801 acgcgacaaa ccgccctgat cgattaacgg tttcagtgcc gccagcagct ggctggtcag
    28861 actgtctggt acggcgatga cattccggtt gcggtaatcg gtcagtacaa acgggtttac
    28921 ttccggcacc accagcggta cgtcgggttc gagagcaaac agtccgctgc tgtcgatcac
    28981 caggcaacct gagttggtcg cttcttcaac ccaggcagcg gtagcttctt tgcctgcgac
    29041 aaaaaatgcc agctgcgcct gcgtccagtc gaattcagcg gcatcctgca cggtgattgt
    29101 tttaccacca aagcgcagtt gttcgcctgc gctttcgtta cgtgccagtg cataaatttc
    29161 cccaaccggg aactgacgtt cagccagcgt ttcaagcagg gcttcgccca cagcgccagt
    29221 tgcgcccagg acggcaatgt tccagccttc agacatggtg gtttactcca gaaatagcaa
    29281 agctccctgc caatgttaca gcagagagca tgaagaagag attaacgtgc cggatgatga
    29341 acggcgttaa aacccagttt acacagcaat gatgccgcac tggcgtcatc acaaattaca
    29401 tacagagatg accattcacg gcgctcaaga tagtttttgc gcagtttatc gaactcaccc
    29461 ggtatcccgg cgactttacg cagcggtgcg tcatcgcggc gcacatcata caccaaatgc
    29521 accagccttt tcagcgtcgg ttgatcgagc gggccatgca gcgtaatgcg accaaactct
    29581 ggcgcgggca gtaatgtatc cagcgcaacg tgctgttcat gcccaataaa cttgctatag
    29641 gcttcaaaca cttgcgtagt accgcgtgct ttaccttcca gggtataacc tgcgatatgc
    29701 ggcgtgccga tatccacttt tgtcagcaac tccacgttaa gttccggttc gccttcccag
    29761 acatccagca ctacgcttaa cttctggcct tcattcaggc aggtcagcaa cgcagtatta
    29821 tcgacgactg cgccacggca ggcgttaatc agaatcgctc ccggcttcag gctacggatc
    29881 agtttttcat ccgccagatg cagcgttttg tacggcccat ctttgaagag tggcgtatgg
    29941 aaagtcagaa tatccgcatg ctgaactaat tcatccagcg agcggaaatc accctcatcc
    30001 ccacggtcag cgcgaggcgg atcgcaaagc aatgttttaa tccctaacgc ttccagtcgc
    30061 gcctgcaatc gacgtccaac gttaccaacg cccacgatcc ccaccgtacg gtcgtgcagt
    30121 gaaaatccat cgcgttcggc aagcatcagt agtgaggaga aaacatattc caccaccgca
    30181 atcgcattac agccaggtgc agcggaaaaa ccaattcccg cctgcttcaa ccatgcttca
    30241 tcgacatggt cggtccccgc agtggcagtg ccaacaaatt taatgggttt tcctgccagc
    30301 aaagattcat tcactttcgt gaccgaacgc accatcagcg catccgcgtc tgccagttga
    30361 gcaacgggga ttggacgccc gggaaccgcg gtcacctcac ccaaacggct aaataagtcg
    30421 cgggcataag gcatattttc atcaacaagg attttcacgt ttgtgttacc tgtatgagac
    30481 gagagttaac cggacaagtg tgccataatc tcgcggccag gcatacttgc gaagatttca
    30541 ggtataagga tacgtaatga tacaacctat ttccggccct cctcctgggc aaccaccagg
    30601 tcagggagat aacctgccgt ctggcgcggg caatcagcct ttatccagtc agcaacgtac
    30661 ttcgctggaa agcttaatga cgaaagtgac ctcactgacg caacaacaaa gagcagaact
    30721 gtgggcgggt atcaggcacg atattggtct gtcgggagat tcaccgctgc tttcgcgtca
    30781 cttccctgcc gctgagcata atctggcgca acgtctgctg gccgcgcaaa aaagccattc
    30841 tgcccgccag cttttagcgc aattagggga gtatttacgt ctggggaata atcgtcaggc
    30901 ggtcacggat tatatccgtc ataactttgg tcagacgccg ctgaatcagc tctcaccgga
    30961 gcaattaaaa accattctca ccctgttgca ggaagggaag atggttattc cgcaaccaca
    31021 gcagcgcgag gcgaccgacc gtcctttatt accggcggag cacaatgcgc taaaacagct
    31081 ggtgaccaaa cttgcggcgg caacggggga acccagcaaa cagatctggc aatcgatgct
    31141 ggaactttcc ggggtgaaag atggcgagtt aattccagcg aaactgttta accatctggt
    31201 gacctggttg caggcgcgcc agacgttaag ccagcaaaat acgccgacgc tggaatcact
    31261 acagatggcg ctaaaacaac ctttagatgc cagtgaactg gcggcgttat cggcatatat
    31321 ccagcaaaaa tatggccttt ctgcgcaatc atcgctttct tctgcccagg ccgaggatat
    31381 tcttaatcag ctttatcaac ggcgggttaa agggattgat ccgcgtgata tgcaaccgct
    31441 gcttaatcct tttccaccga tgatggacac gttgcaaaat atggcaacgc gtcccgcgct
    31501 gtggatactg ttagtcgcga ttatcctgat gctggtctgg ctggttcgtt agccccgacg
    31561 aaatgacagt atcgtgacaa taattcccag caccgcagag atcgccccgg caagaaatac
    31621 cgaagagtaa ccaaacgtgg tcgccagcat tcccgccagc ggcccggaga caccgagggc
    31681 gatatcctga aacgcggcgt aaccgcccag tgcggtgccg cgaacttgtg aggggacgcg
    31741 tttaaccacc tccacgccca gcgcaggaaa gataagcgaa catccggctc cggttaacgc
    31801 cgcgcccgct aatgcgaccc acgcacctgg ggcttgccag agcagcaaca agcccaccgt
    31861 ttctacaagc agagagacaa tcgccacttt cacgccgcca aaacggtccg gcatccagcc
    31921 aaacatgacg cgcatcacga caaatgcgcc gccaaacgcg gtaagagtaa agcccgccat
    31981 cgcccatcct ttgctggcaa agtagagcga aacgaaagtc ccgataaccg caaaaccaac
    32041 gccttgtagt gccagcccta accctggttt ccagataaac ccgacaacgc tccacagcga
    32101 tggacgttct cccgccaggg ccggtacttt gcgcactgtg ccgttacagg cccacgccag
    32161 taagggtaat accattgtgg tgatcgccag tgcggcaaaa ccgtaatggc tatgaatcaa
    32221 caggccaagc ggagcaccaa cagcgagggc accgtaaatc gccattccat tccatgacat
    32281 cactttgcca gagtgttttg gccctacgat gcctaacccc caggtcagag cgcctgtcag
    32341 taactggctt tcaccaaacc caagaatcaa acgcccgatg accaacaggg caaatttgaa
    32401 cggtgcggag acaggcaaaa tcgccgccag cagcaacgcg ccgccagcca gaccacaagc
    32461 taacatcccc tgaagcgccg aacgttttgc accatattga tcggccagtc gcccggcata
    32521 gccacgcgtc agcaccgtag ccagaaactg aatcccgacg gcaatcccga ccatggtatt
    32581 gccatagccc agatcatgat gaacaaacag cgggataacc ggcaacggca gccctacggt
    32641 catgtaggtg agaaaaaccg caaaagcgat gcggaagagc gaaaaattgg cagaagatcg
    32701 tgtttcggtt tggcttacag cagtcatgca ttactccaga atgcagcgca aggcgaggag
    32761 tatccccgtc tcatctctct ggtttcaggg ttacggtgcg ttggcaggat ttaacgcgta
    32821 cgtcttttca gaaggaaatc gacaaagcgg gaagtttgcc tggaacaggc ggcgattgtc
    32881 aatgatgtga aaaagggaac catcaggttc ccttttgcgt tagtgccgga ggccgcatta
    32941 gcgcgtaacg tcggatgcga cgctggcgcg tcttatccga cctactccga ctactccggc
    33001 ctactgcgaa ttaatctttc agcttgcgca ttaccagcgt ggcgttggtg ccgccgaagc
    33061 cgaagctgtt agacataacg gtggtcagtt cgcgatcggt cgtttcggtc acgatgttca
    33121 gacctgcagc ctgctcgtcc agctcttcaa tgttgatgct cggggcgata aagccgtgtt
    33181 ccagcatcag cagagagtag atagcttcct gtacgccagc agcgcccaga gagtgaccgg
    33241 tcatggcttt ggttgcagaa atcgccgggc tcttatcgcc gaacacttca cggatagctg
    33301 ccagttcttt cacgtcgcca accggagtcg aagtaccatg agagttcagg taatcgattg
    33361 gggtatcaac gccgtgcatc gccatcttca tgcagcgtac tgcgccttcg ccagacggag
    33421 caaccatgtc tgcaccatca gaggttgcgc cgtagccaac gatttcagca tagatgtgag
    33481 caccacgcgc cagcgcgtgt tccagctctt caaccactac cataccgccg ccgccagcga
    33541 taacgaaacc gtcacggtga gcgtcgtaag tacgggaggc tttttccggg gtgtcgttgt
    33601 atttagtaga cagcgcaccc atcgcgtcga attcgcaggc catttcccag cacagctctt
    33661 cgccgccgcc agcaaacacg atgtcctgtt tgcccagttg gatctgctct actgcgttac
    33721 cgatacagtg tgcggaagtc gcacacgcgg agctgatgga gtagttaacg ccatgaattt
    33781 taaacggcgt ggcgaggcag gcagaaacgc cggatgccat cgctttggtg accacatacg
    33841 ggccaaccgc tttcaggccg cgcgggccgc gcattgcgtc agcgccgaac acctggaaac
    33901 gcggggaacc gccgccagaa cctgcaatca ggccaacgcg cgggttattc tggtaagctt
    33961 ccggagaaag gcccgcatct gcgattgcct gctccataga aaggaatgca taaatggatg
    34021 cgtcgctcat aaagcgcaca actttgcggt caatgaggcc agtggtatcc agttttacgt
    34081 tgccccagac gtggctacgc atgccggaat ccttcagctc ctgagagaaa gtgatccctg
    34141 aacgtccttc acgcagagat gccaggactt cctgctggtt attaccgatg ctggaaacaa
    34201 tgcccaggcc agtaatcact gcacgtttca ttcaatacct ctgtaagtcg cacatagagt
    34261 aagtttcgaa tgcacaatag cgtacacttg tacgccgaac aagtccgatc agccatttaa
    34321 tagagaaatt tgcgcagcct tacacacatc gctaagatcg agccaccgcc tgtaagacga
    34381 gtaacttacg tgaaacacta ctccatacaa cctgccaacc tcgaatttaa tgctgagggt
    34441 acacctgttt cccgagattt tgacgatgtc tatttttcca acgataacgg gctggaagag
    34501 acgcgttatg tttttctggg aggcaaccaa ttagaggtac gctttcctga gcatccacat
    34561 cctctgtttg tggtagcaga gagcggcttc ggcaccggat taaacttcct gacgctatgg
    34621 caggcattta atcagtttcg cgaagcgcat ccgcaagcgc aattacaacg cttacatttc
    34681 attagttttg agaaatttcc cctcacccgt gcggatttag ccttagcgca tcaacactgg
    34741 ccggaactgg ctccgtgggc agaacaactt caggcgcagt ggccaatgcc cttgcccggt
    34801 tgccatcgtt tattgctcga tgaaggccgc gtgacgctgg atttatggtt tggcgatatt
    34861 aacgaactga ccagccaact ggacgattcg ctaaatcaaa aagtagatgc ctggtttctg
    34921 gacggctttg cgccagcgaa aaacccggat atgtggacgc aaaatctgtt taacgccatg
    34981 gcaaggttgg cgcgtccggg cggcacgctg gcgacattta cgtctgccgg ttttgtccgc
    35041 cgcggtttgc aggacgccgg attcacgatg caaaaacgta agggctttgg gcgcaaacgg
    35101 gaaatgcttt gcggggtgat ggaacagaca ttaccgctcc cctgctccgc gccgtggttt
    35161 aaccgcacgg gcagcagcaa acgggaagcg gcgattatcg gcggtggtat tgccagcgcg
    35221 ttgttgtcgc tggcgctatt acggcgcggc tggcaggtaa cgctttattg cgcggatgag
    35281 gcccccgcac tgggtgcttc cggcaatcgc cagggggcgc tgtatccgtt attaagcaaa
    35341 cacgatgagg cgctaaaccg ctttttctct aatgcgttta cttttgctcg tcggttttac
    35401 gaccaattac ccgttaaatt tgatcatgac tggtgcggcg tcacgcagtt aggctgggat
    35461 gagaaaagcc agcataaaat cgcacagatg ttgtcaatgg atttacccgc agaactggct
    35521 gtagccgttg aggcaaatgc ggttgaacaa attacgggcg ttgcgacaaa ttgcagcggc
    35581 attacttatc cgcaaggtgg ttggctgtgc ccagcagaac tgacccgtaa tgtgctggaa
    35641 ctggcgcaac agcagggttt gcagatttat tatcaatatc agttacagaa tttatcccgt
    35701 aaggatgact gttggttgtt gaattttgca ggagatcagc aagcaacaca cagcgtagtg
    35761 gtactggcga acgggcatca aatcagccga ttcagccaaa cgtcgactct cccggtgtat
    35821 tcggttgccg ggcaggtcag ccatattccg acaacgccgg aactggcaga gctgaagcag
    35881 gtgctgtgct atgacggtta tctcacgcca caaaatccgg cgaatcaaca tcattgtatt
    35941 ggtgccagtt atcatcgcgg cagcgaagat acggcgtaca gtgaggacga tcagcagcag
    36001 aatcgccagc ggttgattga ttgtttcccg caggcacagt gggcaaaaga ggttgatgtc
    36061 agtgataaag aggcgcgctg cggtgtgcgt tgtgccaccc gcgatcatct gccaatggta
    36121 ggcaatgttc ccgattatga ggcaacactc gtggaatatg cgtcgttggc ggagcagaaa
    36181 gatgaggcgg taagcgcgcc ggtttttgac gatctcttta tgtttgcggc tttaggttct
    36241 cgcggtttgt gttctgcccc gctgtgtgcc gagattctgg cggcgcagat gagcgacgaa
    36301 ccgattccga tggatgccag tacgctggcg gcgttaaacc cgaatcggtt atgggtgcgg
    36361 aaattgttga agggtaaagc ggttaaggcg gggtaatctg ctctcgcagt gtttgccgga
    36421 tgcggcgtga acgccttatc cgacctacgt gtgacgtgta ggcctgataa gacgcgataa
    36481 gcgtcgcatc aggcatggtg ctccagacgc cgcaacttac tgctgtgacg cctgctgaaa
    36541 caggttttcc cacatatcgg tcaccagcgc ctggtcacgc ggcgacaatt caccggcacc
    36601 aatggctttt tccagactct ggctaacggt cgtatgcacc gcctgagcgg agtggtcatc
    36661 accactttcc agttctgcga tggctaatgt caggtggcca cgcaaatacc cactggcaaa
    36721 caactcatca tcactggcat ggtcaaccat accgtcgatt aatgccagaa tgcgtgattc
    36781 aaactccgcg atcatcttct ttcctcattg taaaacgtgg caccggcttc agttgagcgc
    36841 ttccggccac gggaactgtt ccgccgtaag ttccggcgtg tgataataat tttgtagtgc
    36901 tttaatgaag cgtgccgggc gttcaggaat gccgtttgct aggtaatcca tcacctgcgc
    36961 atgtacccgc cgttgaaaca ccacacgatc cggttcgaag tcgccttcca gattgtcgca
    37021 actgacgtta aacggatatc ccgccgccac acagaacaac cagtcgaacg cctgcggctt
    37081 cacttcaaca tcttcaaact ggctttgcgt ttgggcatcg cgtccgtccg ggcaatacca
    37141 gtagccgaaa tcaaccagct cacggcgcgc tttcccagca atacaccagt gcgaaatctc
    37201 atgaattgcg ctggcataaa agccatgagc aaagacgatt cggttatacg gtacttccgc
    37261 atcagcagga agatagatcg gttcgtcgtc gcctttaatc agacgggtat taaattcatc
    37321 ggcaaagcag ctattaaaaa tttcaatcaa ctgctcatag tggtgtgtac tgttcattag
    37381 ttcatcccca accagtggag gatctcctgt ccgtggctat caaaaagtaa tttggcactc
    37441 atcaccgctg agacaataac aatcatcggg cggatcagct tttgtccttt gctcaacacc
    37501 agtcgtgaac ccatgcgcgc gccgaggaac tgccccacca gcatgacaaa gcccgtcgcc
    37561 caaatcactt tgccaccgag aataaacagc agtaaaccgc cgatgtttga cgttgcgttg
    37621 agtaatttgg cgtgcgccgt ggctttggcg aggttgaatc cgcacagcgt aacaaaggcc
    37681 agtgcgtaaa acgatccggc tgccggacca aagaatccgt cataaaaacc gacgcagcca
    37741 ccggcaatta acgcgaaggg taaaccgtac atccgacgct ggcgatcttc ttcacccagc
    37801 tttggcatca gcaagaaata gaggccgata caaattacca gaatgggcaa aatctgccgt
    37861 aagacatcag cctgaacgta ttgcaccagc agtgcgccgc tcattgagcc gacaaaggtc
    37921 atggcgatat tgagtttctg atcgcttaaa ctaaccactt tgcggcgaat aaagtagata
    37981 gtagcggaaa tagagccgcc gcaggcttgc agtttattgg ttgccagcgc attagcggga
    38041 gacatccccg ctgccatcaa tgccggaatg gtgagtaacc caccgccacc ggcaatcgag
    38101 tcgataaatc ccgccagcat ggcgacaaaa aagagaactc ccagcaacag cggggaaacc
    38161 ataaacaggc tattaaacgt ttccattaga tcacgtgctc atccagtagc gcctggcagg
    38221 aaggcggcaa cggaggcggt gtcttcttct caggctttgt tgttcccggt tttggaggtt
    38281 caaaccagct ttgcagttct gccccgcaac catcgcctgg tggcggtaaa ggttgatctt
    38341 cacactccag actatcggca ggacaacgta atcgtacatg catatgcgca cgatgctgga
    38401 accagggtcg cactttgcgc aaccagtcgc gatcggtgcc cgcatctagg caaagttgtt
    38461 gtttaatcgc cggattaaca aaaatgcgcg tgacgttttt gtcctgggcg gcgagtttga
    38521 tcaagctgaa aatttctggc ttccacagcg aggagacaac gtgtttaccg tcgcgggaaa
    38581 ctaagtccag tgcttgcggg cgcaagagct gcgcggaggt ccagcgagtt ttcggcagtt
    38641 gcagaaagat atcgacatcc agtccggtct ggtggctggc atgaccgccg ttgaaacgcc
    38701 caccagcagg catccccata tcgccaatca gcaccgtacc catgcccaga ttgctcacct
    38761 ggctactcag acgctggata aacatcacca gatccgggtg accgaaatag cgacgctgat
    38821 cggtacgcat gacctgataa tgttcggact gtatcggcag cgtgtcagcg ccgacaatac
    38881 agccattaga aaaactgcct atcgattgtg cgctacccgg cacaggttgg gttatttttt
    38941 gccacggcgt cgctgccagg ctagcgctac tggcaagcag agccagcagc gcaatcgcgg
    39001 ttttgttcat tttttaccag cgtggaatat cagtcttcac atcggcattt tgcgcccgct
    39061 gccgtaacag gtgatccatt aaaacgatcg ccagcatcgc ttctgcgatc ggcactgcac
    39121 ggatcccgac acagggatcg tgacggcctt tggtgatcat ctcaacttct tcgccaaagc
    39181 ggttaatagt gcgccccggc acggtaatgc tggaggtcgg tttcagcgcc atatgggcaa
    39241 tgatttgctg cccgctgctg ataccgccga gaatgccgcc cgcatggttg ctctggaaac
    39301 cgtctttggt gatttcgtcg cggttctggc tgccgcgcag cgccactacg tcaaaaccgt
    39361 cgccaatttc cacgcctttc accgcgttga tgctcatcag cgcatgggcg atgtcggcat
    39421 ccaggcggtc aaagaccggc tcgccaagtc cggcggggac accactggca acaacggtga
    39481 ctttcgcgcc gatggagtcg ccctcttttt tcagcgcgcg catcagttca tctaacgcgt
    39541 cgattttgtc cgggtccggg cagaagaacg gattttgttc aaccagcgac cagtctttga
    39601 tttccagcgg aatgtcgccc atctgggtca agcagccgcg gatttcaata ccaaatttct
    39661 cggcgagata ttttttggca atcgcccctg ccgccacgcg catggcggtt tcgcgggcgg
    39721 aagaacgtcc accgccgcga taatcgcgca gaccgtattt ttgttcgtag gtgtaatcag
    39781 catggcctgg gcggaaaacg tctttaattg cgctgtaatc ctgagagcgc tggtcagtgt
    39841 tttcgatcaa caagccaatg ctggtgccag tagtaacgcc ttcaaaaaca ccggagagaa
    39901 ttttgacctg atccggctcg cggcgctggg tggtatagcg cgatgtccca gggcgacgac
    39961 ggtcgaggtc atgttgcagg tccgcttccg tcagcggaat gcctggcgga acaccatcga
    40021 cgatgcagcc gagcgccagc ccatgcgatt cgccgaaggt ggttacgcga aagagttgtc
    40081 caattgtgtt tccagccatc acggctccgt tattgttgtg tttgcgtgtt tacttaatct
    40141 ttataaatcg cgaaatattc acgggccgcc agcagctgct ctttggtgag cataaacaca
    40201 ccatcgccgc cgttatcaaa ctccagccag gtgaacggaa catccggata ttgttccata
    40261 agatgtacca tgctgttgcc gacttcacaa atcagcacac catcatcagc aaggtaatct
    40321 gccgcgttac cgagaatgcg acgcgtcagt ttcaggccgt cggtgccaga tgccaggccc
    40381 agttccggct cgtgacggta ttcgtttggc aggtcggaca tatcttccgc gtcgacatac
    40441 ggcgggttag tgacaatcag atcgtattgc actttcggca aatcacggaa cagatcggaa
    40501 cgaatcggaa tgacgttgtg gatcagaccg tgttcttcga tgttctgttc ggcaaccgcc
    40561 agcgcgtcgg gagagatatc taccgcgtcg acttccgctt ccgggaaggc ataagcacag
    40621 gcaatggcga tgcagccgct accggtacac atatctaaaa tatgctgcgg ttgcttgctg
    40681 atgagtccgg caaatttatt gttgatcagt tcaccaatcg gtgagcgcgg caccagcacg
    40741 cgttcatcga cgtaaaattc atgaccgcag aaccaggctt tgttggtcag gtaagccacc
    40801 ggaatgcgct cgttgacgcg gcggatcacg cgttcaacaa tacggtgttt ttcgctggag
    40861 gtcagacgcg cggtgcgcat atcttccggg atatccagcg gcaggtagag cgatggtaac
    40921 accagctgta cggcttcatc ccacgggtta tcggtgccgt gaccgtacca gatatttgcc
    40981 gcgctgaagc ggcttaccga ccagcgcaac atgtcctgaa tggtttgcag ctcatttact
    41041 gcttcatcaa cgaaaatttt atccacgtat tcctccaggg catgatcgca ataatttcgg
    41101 cggctagttt gccatgaaga tgacgataaa tcagcattca cgcgcggtga gtgaggaaaa
    41161 atacgtttaa aacgatcgat tgtgctacga gtcgggtaaa ctgtaggaaa attagaaata
    41221 gagacagata aatgaaaaag aaaacaacac ttagcgagga ggaccaggct ctgtttcgcc
    41281 agttgatggc ggggactcgc aagattaagc aggacacgat tgtccaccga ccgcagcgta
    41341 aaaaaatcag cgaagtgccg gtgaaacgct tgatccagga gcaggctgat gccagccatt
    41401 atttctccga tgagtttcag ccgttattaa ataccgaagg tccggtgaaa tatgttcgcc
    41461 cggatgtcag ccattttgag gcgaagaaac tgcgccgcgg cgattattca ccggaattgt
    41521 ttttggattt acacggtctg acgcagctgc aggccaagca ggaactgggc gcgttgattg
    41581 ccgcctgtcg ccgcgaacat gtgttttgtg cctgcgtgat gcatgggcac gggaagcata
    41641 ttttgaagca acaaacaccg ctgtggctgg cacaacatcc gcatgtgatg gcatttcacc
    41701 aggcaccgaa agagtacggc ggtgatgcgg ctttgttggt gttgattgaa gtggaagagt
    41761 ggttgccgcc ggagttgccc tgaggagttg agcgtgttgt gccaggagcg cggaatggca
    41821 ggggcgaatg accctgccag gtgagaaaag gtgcgctaat acatattcag tatgtaagtg
    41881 aatcggcagg tagaatctat gggctgtcga gatgtcgtgt ccacgcgtag cttcatatca
    41941 tgcagttgcc tggacgtgat cggttgcggc tctggcatca ccgtcacttt ggcattatca
    42001 cagccggaag tcgagaaggt catctgctgt gcgacaaaac ggcagtaggc tccacgctcg
    42061 tcaaaattac tgccatcaca cagtttgccg ttttccatca ggaaagtggg cagtgtttgc
    42121 tcaataacac cacctgttga ctgcaacctg acgactttgt tatgtacaac ataattttca
    42181 ttccaggaaa catcatacgg tgccagggac tctgtcgggc agtcagagct aacttttgtt
    42241 ggtcctataa aaattccggc cccgaaacgc tgatccatag tactggcgct ttcaacataa
    42301 gacgacacaa tccagggaca accgtgtaaa tctccatcgt aataaacgcc aagtactaat
    42361 tcattacatg accaggccgt tactggagag agagcatcat actcttctaa tccggggcag
    42421 gtagaatccg gtaaggttgt attattgcgc tccatcctga gttcagggcc gggaggactc
    42481 ccggtccaac taactaaagg caaacttgct gccgcagttg aattagattc atacgtgcca
    42541 tattcaactg attgaggggt caggactgaa agatgaaaac gatactttgc aacaacgctg
    42601 gtcgtccccg cgggtacata gagtgtggcg atctttgtcg cagcctgtat cccagaagcg
    42661 cacagagcca gcaccaataa acatataaca tgacgtattt tatgcatttt atatatactc
    42721 aaacgtagct gtcaggctgg cggtgaaatc accgatggtt acatcacgac cgcttttcgc
    42781 ctggatccag gcccggaaat taatatcatt gttgccgttg gtaagcgcaa aagtcgcccc
    42841 ggtggtgttg ttaatatcca cccgcttgcc gtcgagcgtt tccatcccta tccccacgcc
    42901 ctgcgcggtg gaggtgtcgt cgactgccag aaaacccggc tgaccgctgt cttccgttcc
    42961 ggtgagggtg actttgacgt tgtatcccgc cggccctttg cagtctttca gcttaaacac
    43021 aaccggcaga tgcgcggact gcccggcgac catcaaatcc cggttgctga cggtgggaaa
    43081 atgaatttcc gccagattgg caccatccac caccagcgtg caggatttac tcagcaggtt
    43141 gccgtaaaaa tgcaggttgt tatcgactgc ccgtgccccc gtgctggcgg tcagcagcag
    43201 gcctgctaac agcgcataac cgggtgaata aatgtgtgct gttttcattg gtaatccacc
    43261 cgtaaggttg cgtaagcaga aaaattcgtg tccgtcagca ccgcatcagc cgctttgacc
    43321 ggtaccgcct ccagctttgg cttctggcta aaatcggtgg cgttaatgga aagcggctcg
    43381 ttgagcttaa aacgctgacc gtcatgctga agctcaattc cgaagcccgg tacatccgtc
    43441 tcaatagcag agtcgttgta ggaggtggca gtccccgtcc aggagagtgt catattccac
    43501 tcgggatcgc ggacatccga gtcgcagctc agttcgtagt ccactttctg acgaccataa
    43561 ttgccatcaa tgctgtcgat aataagatcg cgaaattgca cttcgatggt ttgcccgccg
    43621 ctgatgctac aggcgggcgg cgacatcagc gtaccgtgga atgtgatgtc atcttccgcc
    43681 gccagcacag ccccgcaacc agcgcacagt aaaaggagca ggaatttttt cattggtaat
    43741 ccaccaccat ggtcatcgtg gcgttaaact ccgacggcgt cagttgcacc ccgctctgtt
    43801 ttaccggcac cgctttcagc tgaggctgag tgttgacatt aaaaggcgtc cagttatttt
    43861 caccaactgc aaacagggag ttgtcggcgg cgttttctat ccgcaccccc aggccggcga
    43921 tatccgtgct gagaaccgtt tctccgttga tcacggcggt attgcctttg agctgcatcc
    43981 gtaagtcatc gctgacttta ttaccgcagt tgagggtgta tttcgcatcc tgttggtaat
    44041 tggtaccatc gatcctgttg attatcacat tgccaaattt cacagcgctt cccttcacgg
    44101 agcaaggcgg cggtccatcg accaggattc gcagcgtcag atccacctgt ttgatatcac
    44161 tgccagcaac agccaaaccg ctgatgcccc atccggccag caccagcaac cctgcgcccg
    44221 ctacaaactt gtgactcata agtccctccc ggcatcagcc tttgcggctc tgctcttcat
    44281 ccacctggca gctgttgccg gtgcatatga agtacagcgg catccgcgcg ccgtagtcgt
    44341 tgatgtaagt cagcaccggc acgctgtcca tcttcgcatt cagcggtgcg gaggttttcg
    44401 gctccatcat cagcggtgtg aatcccgccg gcgggttgcc gcctttttta gcgctggcgt
    44461 tgctgatgat cacgtagtac ggcgtcgggt tattgacggt gaagtcctgg ccgttacgga
    44521 tgagcgtcac cttatgctgc cacggacttt tcatgctggt gttctccagc gccttcggtc
    44581 gccagaacag tttgatacgc gtctgcagcg caatctgcag tgtgttcggt ttatttgatt
    44641 tcggcggaat ttcgcgcacg ttgaaataga acacgctctc gcggtcggcc ggcagtttgt
    44701 tgatatccgg cagcccctgc accttcacct ggccgttcat catcgcatcg atacgctgca
    44761 ctggcggaag caccgcaagc ggcgagctga ttttgttgcc ctttgcatcc tctatccagc
    44821 tctgcgccag ataaggcagt gtgggatcgt tgttgcgcag cgtgacgctg atggacttgt
    44881 cgctttcgtt aaacaccagc cgtgtcctgt cgggcgtcac cgaagcgtgg ctggagaaac
    44941 tgaccactgt cgccagcagc gccagtgccg ggacaacgat ttttgcagtg ttagtcagat
    45001 taaacatgga tcattcctta atcacgttac tggtttactg aaattgaagt cggaggttcc
    45061 gttggcgtca cccgctgtgt ctgttcctga atcgccggtt tgaccgccgg cgaagtgata
    45121 ggtgctacat tgcctttatg ctggcatggc agtaacaagc cgttaaacag atcggcaggc
    45181 agcgggtccg gcaggttgat atcgcaatgc gcaacaccac tccagaacac actcatgtgt
    45241 tcgccaggtt tcaccccagc cagataaaca ttgccgtcat catcgacaag gcccactgtc
    45301 tgctcgttat cattttttac ttctgcgcca aacggaggat ggctgccgtc gctcaggcgc
    45361 agcaccgcca tcgctttttg tccgctaatg acagcaaatt tacggtagcc aatggcacct
    45421 tcagttagcg tggcttgcac caccgactgg gttgcttcag cgttttcagg caatctgttg
    45481 aggtcgatat acgcctgatt gcggtaatag ttattgacgt cagaaacgac ggctttacca
    45541 aacatattgg tataaacagc agccccgtta ccttccaccg gaacatcggc aacgccatcg
    45601 gcatcaatca acaatcgtgt cccgcccata ttctgggtac ggtgaagtgc gccaccgtgg
    45661 gcagtaagcg ttgcgccgcc ctgtaacgac aagcccgcag aggtgtattg cccttcatgg
    45721 tagttcgcac tgaggtcaac ctgcgccagc gaaccatcat ggctgtaata gccgtcaacg
    45781 ctggtgtgtt tgtcactggt gccgacgttc aactgatagt gagtcgcgtc atcgacacgg
    45841 ctgaaataac cgacctgact gctgtccgtc ccactgccat agttaccgtt atagctaacg
    45901 gtactgttgt cgccccacgg catactgagc gaaatgtaca tgcctttgtc ggcctggttg
    45961 tcatactcgt aacggtagcc agtcatcgag atgctgacat tacgaatact gcccatattg
    46021 aaatagtgcg agagcatgat gttgtagttt gtctgctcct cgcgatccca gtaggtatgg
    46081 cgggtatagt tgagataaac cgaaacaccc gcatcgcgga agttctggtt ataagtggcg
    46141 gtgtacatct ctttgtcgtt gcccgtgcgt accattccgc tgtcactggc atccagatac
    46201 tcgctcatgg tcataaagtt ctcttccgag aagcgatatc cagcaaaagt aacgcggctg
    46261 ttgagctggt caaagtcttt ggaataactc acacggaagg agttaccgtc cagcgaacct
    46321 ttgccataag cggtgtcttt atccagtttg gtgtgcgagt gagtaacatc aaacgcgacc
    46381 gcgccgaatg tagacaaatc gcgaccgatg ccaagcgccg cagactgata gttttcatct
    46441 cccagtgcgc cgccatataa cgaccagccg ttagcgatcc cccaggaagc ttcagcacca
    46501 gaaaaaaatt caccctcgat atggtgtccc cactcttgcg gacggcccat catgatctta
    46561 tagcgaacct gacctgggcg agtgaggtat ggcatcgagg cggtgctgat gtcatattcc
    46621 tgcacctggc cgttctgttc ttcaatgcga atatgcaacg taccggagac agaatcacca
    46681 agatcctgaa tacgaaacgg tccagccgga acctgcgttt cgtaaatcac acgccccatc
    46741 tggctgacgg tcacttttgc tgtggtgtgt gccacgccgg aaatgtctgg cgcgtagccg
    46801 cgcagattgg gaggcaacat ttgatcgtca gtactgacac tgccaccaac atagttaaag
    46861 ccatcaaaaa tatcggatct gaggtaatcc tcgcccagcg ccagtttggc ttttaatgat
    46921 ggtaacgccc gccaggcata gtagcgactc cactcccatt ttttttgagt ttcatcgccg
    46981 ctgaattcat cgtcatcatc attactgcga gtatgttgat agttagtctg ccagtcagca
    47041 cgcatacgcc acggccccag gttaaccccg accgtcccgt tgccgctgat ctcgttacta
    47101 tcatcaccgc cattttcttc gtgccgggtt tgtgcgttga tgctgtagtc cgcgacgatc
    47161 ccggagatgc cgtcatccca acgtgaaggc ggatcccagt cgggataagt atattcgagg
    47221 taagcctgtg gcagtgaaat gactaatgcg gactggctta aatcagcctt aatttccatg
    47281 ccttccagtt gaccggattt caggcattta gcatcgtggc tccattgcag atttttcgcc
    47341 acgtcttctt ttaaaccaaa ctgcgctacc agttccggtg tcagacaagc atagcttttg
    47401 ctcgcgtcat cttcaccagc ataccagtaa atatcgtact cttccgccaa tggctgttta
    47461 tttagttgaa cctgtaaatt gtattttccg ggctcaacat atccctggct ggaaaaacgc
    47521 ttcagatcaa tttttgtgtc gccttttaat tccagaaaac gggaatcaaa ctgaatgtcg
    47581 tcttcagccc agacactgct ataactccct gacattgcca gcgcaatgca ccacggaaga
    47641 atccgtaatc gaaaaagaga atggtcaggc atacacgttt ctatccgttt ttattcttgt
    47701 tcggtcaaaa tccatttaaa tcccccatag cagtgtcgct atgggggtat gccttacagg
    47761 taagtaatct ggaaggtggt atttgcttcg aaattaccca gatctggcgc atcagctgcg
    47821 ccgaccagcc aggctttaaa gttcagtgtc tgcttcgctt tgcctttatt ggtagaagtg
    47881 tctttcacta ttttctggtc aatacccatg ccgcttttgt aagaggtacc cagagagtca
    47941 ccgatcgcca ggctaacatt gttaaatgcc gcaccggtat cggtattgaa aatggtgtaa
    48001 taattgccgc tatttgcaga agaaacggta ccagtaaagg tagtggtcat tgtttcctga
    48061 gtatcaaata cgcaatcttg cagacgaatc tggaaacttt tcggcgtggt ggtgccgtta
    48121 tttttcagtt tttcggcacc gatatcaccc atatcaactt ccagtttgtg atcgtccgga
    48181 acgatagaac acactgagtc ttcaatggtg ccgtaaaaac gtgcagtacc gttgttaccc
    48241 gcagcgattg ttgccgtaga ggtgaaaacg cccatcacca ttgccgcagc aatagctgtt
    48301 ttaacaaact tactcattat ttgtccttaa actataaata attaaaaata taaacattac
    48361 aatacattga ttcagtcaat acccaatgtt tatacccagc cattgaatac ggcatgcgat
    48421 ccaaaaagaa caggtcgtag aaaattgtat tctttggtgt ggggcc
//
LOCUS       DAZKHF020000007        47640 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2201S31241
            SAMN43267800-rid25106413.denovo.007, whole genome shotgun sequence.
ACCESSION   DAZKHF020000007 DAZKHF020000000
VERSION     DAZKHF020000007.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267800
            Sequence Read Archive: SRR30307263
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 47640)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 47640)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 47640)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350581.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350581.2
            Genome Coverage       :: 151x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:21:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,652
            CDSs (total)                      :: 5,557
            Genes (coding)                    :: 5,320
            CDSs (with protein)               :: 5,320
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 237
            CDSs (without protein)            :: 237
            Pseudo Genes (ambiguous residues) :: 0 of 237
            Pseudo Genes (frameshifted)       :: 102 of 237
            Pseudo Genes (incomplete)         :: 130 of 237
            Pseudo Genes (internal stop)      :: 52 of 237
            Pseudo Genes (multiple problems)  :: 41 of 237
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..47640
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267800-rid25106413.denovo.007"
                     /serotype="O 26:H11"
                     /isolate="2201S31241"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-01"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            67..699
                     /gene="comR"
                     /locus_tag="ACHOSC_RS01490"
                     /old_locus_tag="pgaptmp_000298"
     CDS             67..699
                     /gene="comR"
                     /locus_tag="ACHOSC_RS01490"
                     /old_locus_tag="pgaptmp_000298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415629.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR family copper-responsive transcriptional
                     repressor ComR"
                     /protein_id="HGU4546312.1"
                     /translation="MATDSTQCVKKSRGRPKVFDRDAALDKAMKLFWQHGYEATSLAD
                     LVEATGAKAPTLYAEFTNKEGLFRAVLDRYIDRFAAKHEAQLFCEEKSVESALADYFA
                     AIANCFTSKDTPAGCFMINNCTTLSPDSGDIANTLKSRHAMQERTLQQFLCQRQARGE
                     IPTHCDVTHLAEFLNCIIQGMSISAREGASLEKLMQIAGTTLRLWPELVK"
     gene            complement(761..1300)
                     /gene="ycfJ"
                     /locus_tag="ACHOSC_RS01495"
                     /old_locus_tag="pgaptmp_000299"
     CDS             complement(761..1300)
                     /gene="ycfJ"
                     /locus_tag="ACHOSC_RS01495"
                     /old_locus_tag="pgaptmp_000299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine zipper 2TM domain-containing protein"
                     /protein_id="HGU4546313.1"
                     /translation="MNKSMLAGIGIGVAAALGVAAVASLNVFERGPQYAQVVSATPIK
                     ETVKTPRQECRNVTVTHRRPVQDENRITGSVLGAVAGGVIGHQFGGGRGKDVATVVGA
                     LGGGYAGNQIQGSLQESDTYTTTQQRCKTVYDKSEKMLGYDVTYKIGDQQGKIRMDRD
                     PGTQIPLDSNGQLILNNKV"
     gene            complement(1527..2831)
                     /gene="ndh"
                     /locus_tag="ACHOSC_RS01500"
                     /old_locus_tag="pgaptmp_000300"
     CDS             complement(1527..2831)
                     /gene="ndh"
                     /locus_tag="ACHOSC_RS01500"
                     /old_locus_tag="pgaptmp_000300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017384716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone dehydrogenase"
                     /protein_id="HGU4546314.1"
                     /translation="MTTPLKKIVIVGGGAGGLEMATQLGHKLGRKKKAKITLVDRNHS
                     HLWKPLLHEVATGSLDEGVDALSYLAHARNHGFQFQLGSVIDIDREAKTITIAELRDE
                     KGELLVPERKIAYDTLVMALGSTSNDFNTPGVKENCIFLDNPHQARRFHQEMLNLFLK
                     YSANLGANGKVNIAIVGGGATGVELSAELHNAVKQLHSYGYKGLTNEALNVTLVEAGE
                     RILPALPPRISAAAHNELTKLGVRVLTQTMVTSADEGGLHTKDGEYIEADLMVWAAGI
                     KAPDFLKDIGGLETNRINQLVVEPTLQTTRDPDIYAIGDCASCPRPEGGFVPPRAQAA
                     HQMATCAMNNILAQMNGKPLKNYQYKDHGSLVSLSNFSTVGSLMGNLTRGSMMIEGRI
                     ARFVYISLYRMHQIALHGYFKTGLMMLVGSINRVIRPRLKLH"
     gene            complement(3231..3773)
                     /gene="ycfP"
                     /locus_tag="ACHOSC_RS01505"
                     /old_locus_tag="pgaptmp_000301"
     CDS             complement(3231..3773)
                     /gene="ycfP"
                     /locus_tag="ACHOSC_RS01505"
                     /old_locus_tag="pgaptmp_000301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase YcfP"
                     /protein_id="HGU4546315.1"
                     /translation="MIIYLHGFDSNSPGNHEKVLQLQFIDPDVRLISYSTRHPKHDMQ
                     HLLKEVDKMLQLNVDERPLICGVGLGGYWAERIGFLCDIRQVIFNPNLFPYENMEGKI
                     DRPEEYADIATKCVTNFREKNRDRCLVILSRNDEALNSQRTSEELHHYYEIVWDEEQT
                     HKFKNISPHLQRIKAFKTLG"
     gene            complement(3796..4821)
                     /gene="nagZ"
                     /locus_tag="ACHOSC_RS01510"
                     /old_locus_tag="pgaptmp_000302"
     CDS             complement(3796..4821)
                     /gene="nagZ"
                     /locus_tag="ACHOSC_RS01510"
                     /old_locus_tag="pgaptmp_000302"
                     /EC_number="3.2.1.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-N-acetylhexosaminidase"
                     /protein_id="HGU4546316.1"
                     /translation="MGPVMLDVEGYELDAEEREILAHPLVGGLILFTRNYHDPAQLRE
                     LVRQIRAASRNHLVVAVDQEGGRVQRFREGFTRLPAAQSFAALSGMEEGGKLAQEAGW
                     LMASEMIAMDIDISFAPVLDVGHISAAIGERSYHADPQKALAIASRFIDGMHEAGMKT
                     TGKHFPGHGAVTADSHKETPCDPRPQAEIRAKDMSVFSSLIRENKLDAIMPAHVIYSD
                     VDPRPASGSPYWLKTVLRQELGFDGVIFSDDLSMEGAAIMGSYAERGQASLDAGCDMI
                     LVCNNRKGAVSVLDNLSPIKAERVTRLYHKGSFSRQELMDSARWKAISTRLNQLHERW
                     QEEKAGH"
     gene            complement(4832..5656)
                     /gene="thiK"
                     /locus_tag="ACHOSC_RS01515"
                     /old_locus_tag="pgaptmp_000303"
     CDS             complement(4832..5656)
                     /gene="thiK"
                     /locus_tag="ACHOSC_RS01515"
                     /old_locus_tag="pgaptmp_000303"
                     /EC_number="2.7.1.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019165 - thiamine kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006772 - thiamine metabolic process
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine kinase"
                     /protein_id="HGU4546317.1"
                     /translation="MPFRSNNPIMRDELLSRFFPQFHPVTTFNSGLSGGSFLIEHQGQ
                     RFVVRQPHDPDAPQSAFLRQYRALSQLPASIAPKPHLYLRDWMVVDYLPGEVKTYLPD
                     TNELAGLLYYLHQQPRFGWRITLLPLLELYWQQSDPARRTVGWLRMLKRLRKAREPRP
                     LRLSPLHMDVHAGNLVHSASGLKLIDWEYAGDGDIALELAAVWVENTEQHRQLVNDYA
                     TRAKIYPAQLWRQVRRWFPWLLMLKAGWFEYRWRQTGDQQFIRLADDTWRQLLIKQ"
     gene            complement(5637..6278)
                     /gene="lpoB"
                     /locus_tag="ACHOSC_RS01520"
                     /old_locus_tag="pgaptmp_000304"
     CDS             complement(5637..6278)
                     /gene="lpoB"
                     /locus_tag="ACHOSC_RS01520"
                     /old_locus_tag="pgaptmp_000304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein activator LpoB"
                     /protein_id="HGU4546318.1"
                     /translation="MTKMSRYALITALAMFLAGCVGQREPAPVEEVKPAPEQPAEPQQ
                     PVPTVPSVPTIPQQPGPIEHEDQTAPPAPHIRHYDWNGAMQPMVSKMLGADGVTAGSV
                     LLVDSVNNRTNGSLNAAEATETLRNALANNGKFTLVSAQQLSMAKQQLGLSPQDSLGT
                     RSKAIGIARNVGAHYVLYSSASGNVNAPTLQMQLMLVQTGEIIWSGKGAVSQQ"
     gene            complement(6292..6669)
                     /gene="ycfL"
                     /locus_tag="ACHOSC_RS01525"
                     /old_locus_tag="pgaptmp_000305"
     CDS             complement(6292..6669)
                     /gene="ycfL"
                     /locus_tag="ACHOSC_RS01525"
                     /old_locus_tag="pgaptmp_000305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcfL family protein"
                     /protein_id="HGU4546319.1"
                     /translation="MRKGCFGLVSLALLLLVGCRSHPEIPVNDEQSLVMESSLLAAGI
                     SAEKPVLSTSDIQPSASSTLYNERQEPVTVHYRFYWYDARGLEMHPLERPRSVTIPAH
                     SAVTLYGSANFLGAHKVRLYLYL"
     gene            complement(6672..7031)
                     /gene="hinT"
                     /locus_tag="ACHOSC_RS01530"
                     /old_locus_tag="pgaptmp_000306"
     CDS             complement(6672..7031)
                     /gene="hinT"
                     /locus_tag="ACHOSC_RS01530"
                     /old_locus_tag="pgaptmp_000306"
                     /EC_number="3.9.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415621.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine nucleoside phosphoramidase"
                     /protein_id="HGU4546320.1"
                     /translation="MAEETIFSKIIRREIPSDIVYQDDLVTAFRDISPQAPTHILIIP
                     NILIPTVNDVSAEHEQALGRMITVAAKIAEQEGIAEDGYRLIMNTNRHGGQEVYHIHM
                     HLLGGRPLGPMLAHKGL"
     gene            7365..9554
                     /gene="fhuE"
                     /locus_tag="ACHOSC_RS01535"
                     /old_locus_tag="pgaptmp_000307"
     CDS             7365..9554
                     /gene="fhuE"
                     /locus_tag="ACHOSC_RS01535"
                     /old_locus_tag="pgaptmp_000307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0015344 - siderophore uptake transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0038023 - signaling receptor activity
                     [Evidence IEA];
                     GO_process: GO:0015891 - siderophore transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferric-rhodotorulic acid/ferric-coprogen
                     receptor FhuE"
                     /protein_id="HGU4546321.1"
                     /translation="MLSTQFNRDNQYQASTKPSLLAGCIALALLPSAAFAAPATEETV
                     IVEGSATAPDDGENDYSVTSTSAGTKMQMTQRDIPQSVTIVSQQRMEDQQLQTLGEVM
                     ENTLGISKSQADSDRALYYSRGFQIDNYMVDGIPTYFESRWNLGDALSDMALFERVEV
                     VRGATGLMTGTGNPSAAINMVRKHATSREFKGDVSAEYGSWNKERYVADLQSPLTEDG
                     KIRARIVGGYQNNDSWLDRYNSEKTFFSGIVDADLGDLTTLSAGYEYQHIDVNSPTWG
                     GLPRWNTDGSSNSYDRARSTAPDWAYNDKEINKVFMTLKQRFADTWQATLNATHSEVE
                     FDSKMMYVDAYVNKADGMLIGPYSNYGPGFDYVGGTGWNSGKRKVDALDLFADGSYEL
                     FGRQHNLMFGGSYSKQNNRYFSSWANIFPDEIGSFYNFNGNFPQTDWSPQSLAQDDTT
                     HIKSLYAATRVTLADPLHLILGARYTNWRVDTLTYSMEKNHTTPYAGLVFDINDNWST
                     YASYTSIFQPQNDRDSSGKYLAPITGNNYELGLKSDWMNSRLTTTLAIFRIEQDNVAQ
                     STGTPIPGSNGETAYKAVDGTVSKGVEFELNGAITDNWQLTFGATRYIAEDNEGNAVN
                     PNLPRTTVKMFTSYRLPVMPELTVGGGVNWQNRVYTDTVTPYGTFRAEQGSYALVDLF
                     TRYQVTKNFSLQGNVNNLFDKTYDTNVEGSIVYGAPRNFSITGTYQF"
     gene            complement(9614..11047)
                     /gene="ptsG"
                     /locus_tag="ACHOSC_RS01540"
                     /old_locus_tag="pgaptmp_000308"
     CDS             complement(9614..11047)
                     /gene="ptsG"
                     /locus_tag="ACHOSC_RS01540"
                     /old_locus_tag="pgaptmp_000308"
                     /EC_number="2.7.1.199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000475716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005355 - glucose transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:1904659 - glucose transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIBC"
                     /protein_id="HGU4546322.1"
                     /translation="MFKNAFANLQKVGKSLMLPVSVLPIAGILLGVGSANFSWLPAVV
                     SHVMAEAGGSVFANMPLIFAIGVALGFTNNDGVSALAAVVAYGIMVKTMAVVAPLVLH
                     LPAEEIASKHLADTGVLGGIISGAIAAYMFNRFYRIKLPEYLGFFAGKRFVPIISGLA
                     AIFTGVVLSFIWPPIGSAIQTFSQWAAYQNPVVAFGIYGFIERCLVPFGLHHIWNVPF
                     QMQIGEYTNAAGQVFHGDIPRYMAGDPTAGKLSGGFLFKMYGLPAAAIAIWHSAKPEN
                     RAKVGGIMISAALTSFLTGITEPIEFSFMFVAPILYIIHAILAGLAFPICILLGMRDG
                     TSFSHGLIDFIVLSGNSSKLWLFPIVGIGYAIVYYTIFRVLIKALDLKTPGREDATED
                     AKATGTSEMAPALVAAFGGKENITNLDACITRLRVSVADVSKVDQAGLKKLGAAGVVV
                     AGSGVQAIFGTKSDNLKTEMDEYIRNH"
     gene            complement(11342..12139)
                     /gene="ycfH"
                     /locus_tag="ACHOSC_RS01545"
                     /old_locus_tag="pgaptmp_000309"
     CDS             complement(11342..12139)
                     /gene="ycfH"
                     /locus_tag="ACHOSC_RS01545"
                     /old_locus_tag="pgaptmp_000309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HGU4546323.1"
                     /translation="MFLVDSHCHLDGLDYESLHKDVDDVLAKAAARDVKFCLAVATTL
                     PGYLHMRDLVGERDNVVFSCGVHPLNQNDPYDVEDLRRLAAEEGVVALGETGLDYYYT
                     PETKVRQQESFIHHIQIGRELNKPVIVHTRDARADTLAILREEKVTDCGGVLHCFTED
                     RETAGKLLDLGFYISFSGIVTFRNAEQLRDAARYVPLDRLLVETDSPYLAPVPHRGKE
                     NQPAMVRDVAEYMAVLKGVAVEELAQVTTDNFARLFHIDASRLQSIR"
     gene            complement(12150..13154)
                     /gene="holB"
                     /locus_tag="ACHOSC_RS01550"
                     /old_locus_tag="pgaptmp_000310"
     CDS             complement(12150..13154)
                     /gene="holB"
                     /locus_tag="ACHOSC_RS01550"
                     /old_locus_tag="pgaptmp_000310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta'"
                     /protein_id="HGU4546324.1"
                     /translation="MRWYPWLRPDFEKLVASYQAGRGHHALLIQALPGMGDDALIYAL
                     SRYLLCQQPQGHKSCGHCRGCQLMQAGTHPDYYTLAPEKGKNMLGIDAVREVTEKLNE
                     HARLGGAKVVWVTDAALLTDAAANALLKTLEEPPAETWFFLATREPERLLATLRSRCR
                     LHYLAPPPEQYAVTWLSREVTMSQDALLAALRLSAGSPGAALALFQGDNWQARETLCQ
                     ALAYSVPSGDWYSLLAALNHEQAPARLHWLATLLMDALKRHHGAAQVTNVDVPGLVAE
                     LANHLSPSRLQAILGDVCHIREQLMSVTGINRELLITDLLLRIEHYLQPGVVLPVPHL
                     "
     gene            complement(13151..13792)
                     /gene="tmk"
                     /locus_tag="ACHOSC_RS01555"
                     /old_locus_tag="pgaptmp_000311"
     CDS             complement(13151..13792)
                     /gene="tmk"
                     /locus_tag="ACHOSC_RS01555"
                     /old_locus_tag="pgaptmp_000311"
                     /EC_number="2.7.4.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004798 - thymidylate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006233 - dTDP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTMP kinase"
                     /protein_id="HGU4546325.1"
                     /translation="MRSKYIVIEGLEGAGKTTARNVVVETLEQLGIRDMVFTREPGGT
                     QLAEKLRSLVLDIKSVGDEVITDKAEVLMFYAARVQLVETVIKPALANGTWVIGDRHD
                     LSTQAYQGGGRGIDQHMLATLRDAVLGDFRPDLTLYLDVTPEVGLKRARARGELDRIE
                     QESFDFFNRTRARYLELAAQDKSIHTIDATQPLEAVMDAIRTTVTHWVKELDA"
     gene            complement(13782..14804)
                     /gene="yceG"
                     /locus_tag="ACHOSC_RS01560"
                     /old_locus_tag="pgaptmp_000312"
     CDS             complement(13782..14804)
                     /gene="yceG"
                     /locus_tag="ACHOSC_RS01560"
                     /old_locus_tag="pgaptmp_000312"
                     /EC_number="4.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein YceG"
                     /protein_id="HGU4546326.1"
                     /translation="MKKVLLIILLLLVVLGIAAGVGVWKVRHLADSKLLIKEETIFTL
                     KPGTGRLALGEQLYADKIINRPRVFQWLLRIEPDLSHFKAGTYRFTPQMTVREMLKLL
                     ESGKEAQFPLRLVEGMRLSDYLKQLREAPYIKHTLSDDKYATVAQALELENPEWIEGW
                     FWPDTWMYTANTTDVALLKRAHKKMVKAVDSAWEGRADGLPYKDKNQLVTMASIIEKE
                     TAVASERDQVASVFINRLRIGMRLQTDPTVIYGMGERYNGKLSRADLETPTAYNTYTI
                     TGLPPGAIATPGADSLKAAAHPAKTPYLYFVADGKGGHTFNTNLASHNKSVQDYLKVL
                     KEKNAQ"
     gene            complement(14807..15616)
                     /gene="pabC"
                     /locus_tag="ACHOSC_RS01565"
                     /old_locus_tag="pgaptmp_000313"
     CDS             complement(14807..15616)
                     /gene="pabC"
                     /locus_tag="ACHOSC_RS01565"
                     /old_locus_tag="pgaptmp_000313"
                     /EC_number="4.1.3.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008696 - 4-amino-4-deoxychorismate lyase
                     activity [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0046656 - folic acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminodeoxychorismate lyase"
                     /protein_id="HGU4546327.1"
                     /translation="MFLINGYKQESLAVSDRATQFGDGCFTTARVIDGKVSLLSAHIQ
                     RLQDACQRLMIFCDFWPQLEQEMKTLAAEQQNGVLKVVISRGSGGRGYSTLNSGPATR
                     ILSVTVYPAHYDRLRNEGMTLALSPVRLGRNPHLAGIKHLNRLEQVLIRSHLEQTNAD
                     EALVLDSEGWVTECCAANLFWRKGNVVYTPRLDQAGVNGIMRQFCIRLLAQSSYQLVE
                     VQASLEEALQADEMVICNALMPVMPVRACGDVSFSSATLYEYLAPLCERPN"
     gene            complement(15736..16977)
                     /gene="fabF"
                     /locus_tag="ACHOSC_RS01570"
                     /old_locus_tag="pgaptmp_000314"
     CDS             complement(15736..16977)
                     /gene="fabF"
                     /locus_tag="ACHOSC_RS01570"
                     /old_locus_tag="pgaptmp_000314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase II"
                     /protein_id="HGU4546328.1"
                     /translation="MSKRRVVVTGLGMLSPVGNTVESTWKALLAGQSGISLIDHFDTS
                     AYATKFAGLVKDFNCEDIISRKEQRKMDAFIQYGIVAGVQAMQDSGLEITEENATRIG
                     AAIGSGIGGLGLIEENHTSLMNGGPRKISPFFVPSTIVNMVAGHLTIMYGLRGPSISI
                     ATACTSGVHNIGHAARIIAYGDADVMVAGGAEKASTPLGVGGFGAARALSTRNDNPQA
                     ASRPWDKERDGFVLGDGAGMLVLEEYEHAKKRGAKIYAELVGFGMSSDAYHMTSPPEN
                     GAGAALAMANALRDAGIEASQIGYVNAHGTSTPAGDKAEAQAVKTIFGEAASRVLVSS
                     TKSMTGHLLGAAGAVESIYSILALRDQAVPPTINLDNPDEGCDLDFVPHEARQVSGME
                     YTLCNSFGFGGTNGSLIFKKI"
     gene            complement(17065..17301)
                     /gene="acpP"
                     /locus_tag="ACHOSC_RS01575"
                     /old_locus_tag="pgaptmp_000315"
     CDS             complement(17065..17301)
                     /gene="acpP"
                     /locus_tag="ACHOSC_RS01575"
                     /old_locus_tag="pgaptmp_000315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016535861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HGU4546329.1"
                     /translation="MSTIEERVKKIIGEQLGVKQEEVTNNASFVEDLGADSLDTVELV
                     MALEEEFDTEIPDEEAEKITTVQAAIDYINGHQA"
     gene            complement(17512..18246)
                     /gene="fabG"
                     /locus_tag="ACHOSC_RS01580"
                     /old_locus_tag="pgaptmp_000316"
     CDS             complement(17512..18246)
                     /gene="fabG"
                     /locus_tag="ACHOSC_RS01580"
                     /old_locus_tag="pgaptmp_000316"
                     /EC_number="1.1.1.100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004316 - 3-oxoacyl-[acyl-carrier-protein]
                     reductase (NADPH) activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-ACP reductase FabG"
                     /protein_id="HGU4546330.1"
                     /translation="MNFEGKIALVTGASRGIGRAIAETLAARGAKVIGTATSENGAQA
                     ISDYLGANGKGLMLNVTDPASIESVLEKIRAEFGEVDILVNNAGITRDNLLMRMKDEE
                     WNEIIETNLSSVFRLSKAVMRAMMKKRHGRIITIGSVVGTMGNGGQANYAAAKAGLIG
                     FSKSLAREVASRGITVNVVAPGFIETDMTRALSDDQRAGILAQVPAGRLGGAQEIANA
                     VAFLASDEAAYITGETLHVNGGMYMV"
     gene            complement(18259..19188)
                     /gene="fabD"
                     /locus_tag="ACHOSC_RS01585"
                     /old_locus_tag="pgaptmp_000317"
     CDS             complement(18259..19188)
                     /gene="fabD"
                     /locus_tag="ACHOSC_RS01585"
                     /old_locus_tag="pgaptmp_000317"
                     /EC_number="2.3.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004314 - [acyl-carrier-protein]
                     S-malonyltransferase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ACP S-malonyltransferase"
                     /protein_id="HGU4546331.1"
                     /translation="MTQFAFVFPGQGSQTVGMLADMAASYPIVEETFAEASAALGYDL
                     WALTQQGPAEELNKTWQTQPALLIASVALYRVWQQQGGKAPAMMAGHSLGEYSALVCA
                     GVINFADAVRLVEMRGKFMQEAVPEGTGAMAAIIGLDDASIAKACEEAAEGQVVSPVN
                     FNSPGQVVIAGHKEAVERAGAACKAAGAKRALPLPVSVPSHCALMKPAADKLAVELAK
                     ITFNAPTVSVVNNVDVKCETNGDAIRDALVRQLYNPVQWTKSVEYMAAQGVEHLYEVG
                     PGKVLTGLTKRIVDTLTASALNEPSAMAAALEL"
     gene            complement(19204..20157)
                     /gene="fabH"
                     /locus_tag="ACHOSC_RS01590"
                     /old_locus_tag="pgaptmp_000318"
     CDS             complement(19204..20157)
                     /gene="fabH"
                     /locus_tag="ACHOSC_RS01590"
                     /old_locus_tag="pgaptmp_000318"
                     /EC_number="2.3.1.180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase III"
                     /protein_id="HGU4546332.1"
                     /translation="MYTKIIGTGSYLPEQVRTNADLEKMVDTSDEWIVTRTGIRERHI
                     AAPNETVSTMGFEAATRAIEMAGIEKDQIGLIVVATTSATHAFPSAACQIQSMLGIKG
                     CPAFDVAAACAGFTYALSVADQYVKSGAVKYALVVGSDVLARTCDPTDRGTIIIFGDG
                     AGAAVLAASEEPGIISTHLHADGSYGELLTLPNADRVNPENSIHLTMAGNEVFKVAVT
                     ELAHIVDETLAANNLDRSQLDWLVPHQANLRIISATAKKLGMSMDNVVVTLDRHGNTS
                     AASVPCALDEAVRDGRIKPGQLVLLEAFGGGFTWGSALVRF"
     gene            complement(20225..21295)
                     /gene="plsX"
                     /locus_tag="ACHOSC_RS01595"
                     /old_locus_tag="pgaptmp_000319"
     CDS             complement(20225..21295)
                     /gene="plsX"
                     /locus_tag="ACHOSC_RS01595"
                     /old_locus_tag="pgaptmp_000319"
                     /EC_number="2.3.1.274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415608.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043811 - phosphate:acyl-[acyl carrier
                     protein] acyltransferase activity [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acyltransferase PlsX"
                     /protein_id="HGU4546333.1"
                     /translation="MTRLTLALDVMGGDFGPSVTVPAALQALNSNSQLTLLLVGNPDA
                     ITPLLAKADFEQRSRLQIIPAQSVIASDARPSQAIRASRGSSMRMALELVKEGRAQAC
                     VSAGNTGALMGLAKLLLKPLEGIERPALVTVLPHQQKGKTVVLDLGANVDCDSTMLVQ
                     FAIMGSVLAEEVVEIPNPRVALLNIGEEEVKGLDSIRDASAVLKTIPSINYIGYLEAN
                     ELLTGKTDVLVCDGFTGNVTLKTMEGVVRMFLSLLKSQGEGKKRSWWLLLLKRWLQKS
                     LTRRFSHLNPDQYNGACLLGLRGTVIKSHGAANQRAFAVAIEQAVQAVQRQVPQRIAA
                     RLESVYPAGFELLDGGKSGTLR"
     gene            complement(21376..21549)
                     /gene="rpmF"
                     /locus_tag="ACHOSC_RS01600"
                     /old_locus_tag="pgaptmp_000320"
     CDS             complement(21376..21549)
                     /gene="rpmF"
                     /locus_tag="ACHOSC_RS01600"
                     /old_locus_tag="pgaptmp_000320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005125993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L32"
                     /protein_id="HGU4546334.1"
                     /translation="MAVQQNKPTRSKRGMRRSHDALTAVTSLSVDKTSGEKHLRHHIT
                     ADGYYRGRKVIAK"
     gene            complement(21601..22122)
                     /gene="yceD"
                     /locus_tag="ACHOSC_RS01605"
                     /old_locus_tag="pgaptmp_000321"
     CDS             complement(21601..22122)
                     /gene="yceD"
                     /locus_tag="ACHOSC_RS01605"
                     /old_locus_tag="pgaptmp_000321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001174481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA accumulation protein YceD"
                     /protein_id="HGU4546335.1"
                     /translation="MQKVKLPLTLDPVRTAQKRLDYQGIYTPDQVERVAESVVSVDSD
                     VECSMSFAIDNQRLAVLNGDAKVTVTLECQRCGKPFTHQVYTTYCFSPVRSDEQAEAL
                     PEAYEPIEVNEFGEIDLLAMVEDEIILALPVVPVHDSEHCEVSEADMVFGELPEEAQK
                     PNPFAVLASLKRK"
     gene            22321..22905
                     /gene="yceF"
                     /locus_tag="ACHOSC_RS01610"
                     /old_locus_tag="pgaptmp_000322"
     CDS             22321..22905
                     /gene="yceF"
                     /locus_tag="ACHOSC_RS01610"
                     /old_locus_tag="pgaptmp_000322"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415605.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="7-methyl-GTP pyrophosphatase"
                     /protein_id="HGU4546336.1"
                     /translation="MPKLILASTSPWRRALLEKLQISFECAAPEVDETPRSDESPRQL
                     VLRLAQEKAQSLASRYPDHLIIGSDQVCVLDGEITGKPLTEENARLQLRKASGNIVTF
                     YTGLALFNSANGHLQTEVEPFDVHFRHLSEAEIDNYVRKEHPLHCAGSFKSEGFGITL
                     FERLEGRDPNTLVGLPLIALCQMLRREGKNPLMG"
     gene            complement(23017..23976)
                     /gene="rluC"
                     /locus_tag="ACHOSC_RS01615"
                     /old_locus_tag="pgaptmp_000323"
     CDS             complement(23017..23976)
                     /gene="rluC"
                     /locus_tag="ACHOSC_RS01615"
                     /old_locus_tag="pgaptmp_000323"
                     /EC_number="5.4.99.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA pseudouridine(955/2504/2580) synthase
                     RluC"
                     /protein_id="HGU4546337.1"
                     /translation="MKTETPSVKIVAITADEAGQRIDNFLRTQLKGVPKSMIYRILRK
                     GEVRVNKKRIKPEYKLEAGDEVRIPPVRVAEREEEAVSPHLQKVAALADVILYEDDHI
                     LVLNKPSGTAVHGGSGLSFGVIEGLRALRPEARFLELVHRLDRDTSGVLLVAKKRSAL
                     RSLHEQLREKGMQKDYLALVRGQWQSHVKSVQAPLLKNILQSGERIVRVSQEGKPSET
                     RFKVEERYAFATLVRCSPVTGRTHQIRVHTQYAGHPIAFDDRYGDREFDRQLTEAGTG
                     LNRLFLHAAALKFTHPGTGEVMRIEAPMDEGLKRCLQKLRNAR"
     gene            24549..27734
                     /gene="rne"
                     /locus_tag="ACHOSC_RS01620"
                     /old_locus_tag="pgaptmp_000324"
     CDS             24549..27734
                     /gene="rne"
                     /locus_tag="ACHOSC_RS01620"
                     /old_locus_tag="pgaptmp_000324"
                     /EC_number="3.1.26.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0008995 - ribonuclease E activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease E"
                     /protein_id="HGU4546338.1"
                     /translation="MKRMLINATQQEELRVALVDGQRLYDLDIESPGHEQKKANIYKG
                     KITRIEPSLEAAFVDYGAERHGFLPLKEIAREYFPANYSAHGRPNIKDVLREGQEVIV
                     QIDKEERGNKGAALTTFISLAGSYLVLMPNNPRAGGISRRIEGDDRTELKEALASLEL
                     PEGMGLIVRTAGVGKSAEALQWDLSFRLKHWEAIKKAAESRPAPFLIHQESNVIVRAF
                     RDYLRQDIGEILIDNPKVLELARQHIAALGRPDFSSKIKLYTGEIPLFSHYQIESQIE
                     SAFQREVRLPSGGSIVIDSTEALTAIDINSARATRGGDIEETAFNTNLEAADEIARQL
                     RLRDLGGLIVIDFIDMTPVRHQRAVENRLREAVRQDRARIQISHISRFGLLEMSRQRL
                     SPSLGESSHHVCPRCSGTGTVRDNESLSLSILRLIEEEALKENTQEVHAIVPVPIASY
                     LLNEKRSAVNAIETRQDGVRCVIVPNDQMETPHYHVLRVRKGEETPTLSYMLPKLHEE
                     AMALPSEEEFAERKRPEQPALATFAMPDVPPAPTPAEPAAPVVAPAPKAAPATPATPA
                     QPGLLSRFFGALKALFSGSEETKPTEQPAPKAEAKPERQQDRRKPRQNNRRDRNERRD
                     TRSERTEGSDNREENRRNRRQAQQQTAETRESRQQAEVTEKARTTDEQQAPRRERSRR
                     RNDDKRQAQQEAKALNVEEQSVQETEQEERVRPVQPRRKQRQLNQKVRYEQSVAEEAV
                     VAPVVEETAAAEPIVQEAPAPRTELVKVPLPVVAQTAPEQQEENNADNRDNGGMPRRS
                     RRSPRHLRVSGQRRRRYRDERYPTQSPMPLTVACASPELASGKVWIRYPIVRPQDVQV
                     EEQREQEEVQVQPMVTEVPVAAAVEPVVSAPVVEEMAEVVEAPVPVAEPQPEVVETTH
                     PEVIAAAVTEQPQVITESDVAVAQEVAEHAEPVVEPQEETADIEEVAETAEVVVAEPE
                     VVAQPAAPVVAEVAAEVETVAAVEPEITVEHNHATAPMTRAPAPEYVPEAPRHSDWQR
                     PTFAFEGKGAAGGHTATHHASAAPARPQPVE"
     gene            complement(27930..28883)
                     /gene="flgL"
                     /locus_tag="ACHOSC_RS01625"
                     /old_locus_tag="pgaptmp_000325"
     CDS             complement(27930..28883)
                     /gene="flgL"
                     /locus_tag="ACHOSC_RS01625"
                     /old_locus_tag="pgaptmp_000325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009424 - bacterial-type flagellum hook
                     [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-associated protein FlgL"
                     /protein_id="HGU4546339.1"
                     /translation="MRFSTQMMYQQNMRGITNSQAEWMKYGEQMSTGKRVVNPSDDPI
                     AASQAVVLSQAQAQNSQYTLARTFATQKVSLEESVLSQVTTAIQNAQEKIVYASNGTL
                     SDDDRASLATDIQGLRDQLLNLANTTDGNGRYIFAGYKTETAPFSEEKGKYVGGAESI
                     RQQVDASRSMVIGHTGDKIFDSITSNAVAEPDGSASETNLFAMLDSAIAALKTPVADS
                     EADKEIAAAALDKTNRGLKNSLNNVLTVRAELGTQLNELESLDSLGSDRALGQTQQMS
                     DLVDVDWNATISSYIMQQTALQASYKAFTDMQGLSLFQLNK"
     gene            complement(28895..30538)
                     /gene="flgK"
                     /locus_tag="ACHOSC_RS01630"
                     /old_locus_tag="pgaptmp_000326"
     CDS             complement(28895..30538)
                     /gene="flgK"
                     /locus_tag="ACHOSC_RS01630"
                     /old_locus_tag="pgaptmp_000326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009424 - bacterial-type flagellum hook
                     [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0044780 - bacterial-type flagellum assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-associated protein FlgK"
                     /protein_id="HGU4546340.1"
                     /translation="MSSLINNAMSGLNAAQAALNTASNNISSYNVAGYTRQTTIMAQA
                     NSTLGAGGWVGNGVYVSGVQREYDAFITNQLRAAQTQSSGLTARYEQMSKIDNMLSTS
                     TSSLATQMQDFFTSLQTLVSNAEDPAARQALIGKSEGLVNQFKTTDQYLRDQDKQVNI
                     AIGASVDQINNYAKQIASLNDQISRLTGVGAGASPNNLLDQRDQLVSELNQIVGVEVS
                     VQDGGTYNITMANGYSLVQGSTARQLAAVPSSADPSRTTVAYVDGTAGNIEIPEKLLN
                     TGSLGGILTFRSQDLDQTRNTLGQLALAFAEAFNSQHKAGFDANGDAGEDFFAIGKPA
                     VLQNTKNTGDVAIGATVTDASAVLATDYKISFDNNQWQVTRLASNTTFTVTPDANGKV
                     AFDGLELTFTGTPAVNDSFTLKPVSDAIVNMDVLITDEAKIAMASEEDAGDSDNRNGQ
                     ALLDLQSNSKTVGGAKSFNDAYASLVSDIGNKTATLKTSSATQGNVVTQLSNQQQSIS
                     GVNLDEEYGNLQRFQQYYLANAQVLQTANAIFDALINIR"
     gene            complement(30604..31545)
                     /gene="flgJ"
                     /locus_tag="ACHOSC_RS01635"
                     /old_locus_tag="pgaptmp_000327"
     CDS             complement(30604..31545)
                     /gene="flgJ"
                     /locus_tag="ACHOSC_RS01635"
                     /old_locus_tag="pgaptmp_000327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA];
                     GO_process: GO:0044780 - bacterial-type flagellum assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar assembly peptidoglycan hydrolase FlgJ"
                     /protein_id="HGU4546341.1"
                     /translation="MISDSKLLASAAWDAQSLNELKAKAGEDPAANIRPVARQVEGMF
                     VQMMLKSMRDALPKDGLFSSEHTRLYTSMYDQQIAQQMTAGKGLGLAEMMVKQMTPEQ
                     PLPEESTPAAPMKFPLETVVRYQNQALSQLVQKAVPRNYDDSLPGDSKAFLAQLSLPA
                     QLASQQSGVPHHLILAQAALESGWGQRQIRRENGEPSYNLFGVKASGNWKGPVTEITT
                     TEYENGEAKKVKAKFRVYSSYLEALSDYVGLLTRNPRYAAVTTAASAEQGAQALQDAG
                     YATDPHYARKLTNMIQQMKSISDKVSKTYSMNIDNLF"
     gene            complement(31545..32642)
                     /gene="flgI"
                     /locus_tag="ACHOSC_RS01640"
                     /old_locus_tag="pgaptmp_000328"
     CDS             complement(31545..32642)
                     /gene="flgI"
                     /locus_tag="ACHOSC_RS01640"
                     /old_locus_tag="pgaptmp_000328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415598.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body P-ring protein FlgI"
                     /protein_id="HGU4546342.1"
                     /translation="MIKFLSALILLLVTTAAQAERIRDLTSVQGVRQNSLIGYGLVVG
                     LDGTGDQTTQTPFTTQTLNNMLSQLGITVPTGTNMQLKNVAAVMVTASLPPFGRQGQT
                     IDVVVSSMGNAKSLRGGTLLMTPLKGVDSQVYALAQGNILVGGAGASAGGSSVQVNQL
                     NGGRITNGAVIERELPSQFGVGNTLNLQLNDEDFSMAQQIADTINRVRGYGSATALDA
                     RTIQVRVPSGNSSLVRFLADIQNMQVNVTPQDAKVVINSRTGSVVMNREVTLDSCAVA
                     QGNLSVTVNRQANVSQPDTPFGGGQTVVTPQTQIDLRQSGGSLQSVRSSASLNNVVRA
                     LNALGATPMDLMSILQSMQSAGCLRAKLEII"
     gene            complement(32654..33352)
                     /gene="flgH"
                     /locus_tag="ACHOSC_RS01645"
                     /old_locus_tag="pgaptmp_000329"
     CDS             complement(32654..33352)
                     /gene="flgH"
                     /locus_tag="ACHOSC_RS01645"
                     /old_locus_tag="pgaptmp_000329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009427 - bacterial-type flagellum basal
                     body, distal rod, L ring [Evidence IEA];
                     GO_function: GO:0003774 - cytoskeletal motor activity
                     [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body L-ring protein FlgH"
                     /protein_id="HGU4546343.1"
                     /translation="MQKNAAHTYAISSLLVLSLTGCAWIPSTPLVQGATSAQPVPGPT
                     PVANGSIFQSAQPINYGYQPLFEDRRPRNIGDTLTIVLQENVSASKSSSANASRDGKT
                     NFGFDTVPRYLQGLFGNARADVEASGGNTFNGKGGANASNTFSGTLTVTVDQVLVNGN
                     LHVVGEKQIAINQGTEFIRFSGVVNPRTISGSNTVPSTQVADARIEYVGNGYINEAQN
                     MGWLQRFFLNLSPM"
     gene            complement(33405..34187)
                     /gene="flgG"
                     /locus_tag="ACHOSC_RS01650"
                     /old_locus_tag="pgaptmp_000330"
     CDS             complement(33405..34187)
                     /gene="flgG"
                     /locus_tag="ACHOSC_RS01650"
                     /old_locus_tag="pgaptmp_000330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006819466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009426 - bacterial-type flagellum basal
                     body, distal rod [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal-body rod protein FlgG"
                     /protein_id="HGU4546344.1"
                     /translation="MISSLWIAKTGLDAQQTNMDVIANNLANVSTNGFKRQRAVFEDL
                     LYQTIRQPGAQSSEQTTLPSGLQIGTGVRPVATERLHSQGNLSQTNNSKDVAIKGQGF
                     FQVMLPDGSSAYTRDGSFQVDQNGQLVTAGGFQVQPAITIPANALSITIGRDGVVSVT
                     QQGQAAPVQVGQLNLTTFMNDTGLESIGENLYTETQSSGAPNESTPGLNGAGLLYQGY
                     VETSNVNVAEELVNMIQVQRAYEINSKAVSTTDQMLQKLTQL"
     gene            complement(34325..35080)
                     /gene="flgF"
                     /locus_tag="ACHOSC_RS01655"
                     /old_locus_tag="pgaptmp_000331"
     CDS             complement(34325..35080)
                     /gene="flgF"
                     /locus_tag="ACHOSC_RS01655"
                     /old_locus_tag="pgaptmp_000331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal-body rod protein FlgF"
                     /protein_id="HGU4546345.1"
                     /translation="MDHAIYTAMGAASQTLNQQAVTASNLANASTPGFRAQLNALRAV
                     PVEGLSLPTRTLVTASTPGADMTPGKMDYTSRPLDVALQQDGWLAVQSADGSEGYTRN
                     GSIQVDPTGQLTIQGHPVIGEAGPIAVPEGAEITIAADGTISALNPGDPANTVAPVGR
                     LKLVKATGSEVQRGDDGIFRLSAESQATRGPILQADPTLRVMSGVLEGSNVNAVAAMS
                     DMIASARRFEMQMKVISSVDDNAGRANQLLSMS"
     gene            complement(35100..36305)
                     /gene="flgE"
                     /locus_tag="ACHOSC_RS01660"
                     /old_locus_tag="pgaptmp_000332"
     CDS             complement(35100..36305)
                     /gene="flgE"
                     /locus_tag="ACHOSC_RS01660"
                     /old_locus_tag="pgaptmp_000332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook protein FlgE"
                     /protein_id="HGU4546346.1"
                     /translation="MAFSQAVSGLNAAATNLDVIGNNIANSATYGFKSGTASFADMFA
                     GSKVGLGVKVAGITQDFTDGTTTNTGRGLDVAISQNGFFRLVDSNGSVFYSRNGQFKL
                     DENRNLVNMQGLQLTGYPATGTPPTIQQGANPTNISIPNTLMAAKTTTTASMQINLNS
                     SDPLPTVTPFSASNADSYNKKGSVTVFDSQGNAHDMSVYFVKTGDNNWQVYTQDSSDP
                     TGTAEPAMKLVFNANGVLTSNPTENITTGAINGADPATFSLSFLNSMQQNTGANNIVA
                     TTQNGYKPGDLVSYQINDDGTVVGNYSNEQTQLLGQIVLANFANNEGLASEGDNVWSA
                     TQSSGVALLGTAGTGNFGTLTNGALEASNVDLSKELVNMIVAQRNYQSNAQTIKTQDQ
                     ILNTLVNLR"
     gene            complement(36330..37025)
                     /gene="flgD"
                     /locus_tag="ACHOSC_RS01665"
                     /old_locus_tag="pgaptmp_000333"
     CDS             complement(36330..37025)
                     /gene="flgD"
                     /locus_tag="ACHOSC_RS01665"
                     /old_locus_tag="pgaptmp_000333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook assembly protein FlgD"
                     /protein_id="HGU4546347.1"
                     /translation="MSIAVTTTDPTNTGVSTTSSSSLTGSNAADLQSSFLTLLVAQLK
                     NQDPTNPMENNELTSQLAQISTVSGIEKLNTTLGSISGQIDNSQSLQASNLIGHGVMI
                     PGTTVLAGTGSEEGAVTTTTPFGVELQQAADKVTATITDKNGAVVRTIDIGELTAGVH
                     SFTWDGTLTDGSTAPNGSYNVAISASNGGTQLVAQPLQFALVQGVIRGNSGNTLDLGT
                     YGTTTLDEVRQII"
     gene            complement(37037..37441)
                     /gene="flgC"
                     /locus_tag="ACHOSC_RS01670"
                     /old_locus_tag="pgaptmp_000334"
     CDS             complement(37037..37441)
                     /gene="flgC"
                     /locus_tag="ACHOSC_RS01670"
                     /old_locus_tag="pgaptmp_000334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001740734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030694 - bacterial-type flagellum basal
                     body, rod [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body rod protein FlgC"
                     /protein_id="HGU4546348.1"
                     /translation="MALLNIFDIAGSALTAQSQRLNVAASNLANADSVTGPDGQPYRA
                     KQVVFQVNAAPGAATGGVKVADVIESQAPDKLVYEPGNPLADAKGYVKMPNVDVVGEM
                     VNTMSASRSYQANVEVLNTVKSMMLKTLTLGQ"
     gene            complement(37445..37861)
                     /gene="flgB"
                     /locus_tag="ACHOSC_RS01675"
                     /old_locus_tag="pgaptmp_000335"
     CDS             complement(37445..37861)
                     /gene="flgB"
                     /locus_tag="ACHOSC_RS01675"
                     /old_locus_tag="pgaptmp_000335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030694 - bacterial-type flagellum basal
                     body, rod [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body rod protein FlgB"
                     /protein_id="HGU4546349.1"
                     /translation="MLDKLDAALRFQQEALNLRAQRQEVLAANIANADTPGYQARDID
                     FASELKKVMQRGRDATSVVALTMTSTQHIPAQALTPPTAELQYRIPDQPSLDGNTVDM
                     DRERTQFADNSLQYQMSLSALSGQIKGMMNVLQSGN"
     gene            38016..38675
                     /gene="flgA"
                     /locus_tag="ACHOSC_RS01680"
                     /old_locus_tag="pgaptmp_000336"
     CDS             38016..38675
                     /gene="flgA"
                     /locus_tag="ACHOSC_RS01680"
                     /old_locus_tag="pgaptmp_000336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009428 - bacterial-type flagellum basal
                     body, distal rod, P ring [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body P-ring formation chaperone
                     FlgA"
                     /protein_id="HGU4546350.1"
                     /translation="MLTIKRSVAIIAILFSPLSTASNLTSQLHNFFSAQLAGVSDEVR
                     VSIRTAPNLLPPCEQPLLSMSNNSRLWGNVNVLARCGNDKRYLQVNVQATGNYVVAAM
                     PIVRGGKLEAGNVKLKRGRLDTLPPRTVLDINQLVDAISLRDLSPDQPIQLTQFRQAW
                     RVKAGQRVNVIASGDGFSANAEGQALNNAAVAQNARVRMVSGQVVSGVVDADGNILIN
                     L"
     gene            38751..39044
                     /gene="flgM"
                     /locus_tag="ACHOSC_RS01685"
                     /old_locus_tag="pgaptmp_000337"
     CDS             38751..39044
                     /gene="flgM"
                     /locus_tag="ACHOSC_RS01685"
                     /old_locus_tag="pgaptmp_000337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis anti-sigma factor FlgM"
                     /protein_id="HGU4546351.1"
                     /translation="MSIDRTSPLKPVSTVQPRETTDAPVTNSRAAKTTASTSTSVTLS
                     DAQAKLMQPGSSDINLERVEALKLAIRNGELKMDTGKIADALINEAQQDLQSN"
     gene            39049..39465
                     /gene="flgN"
                     /locus_tag="ACHOSC_RS01690"
                     /old_locus_tag="pgaptmp_000338"
     CDS             39049..39465
                     /gene="flgN"
                     /locus_tag="ACHOSC_RS01690"
                     /old_locus_tag="pgaptmp_000338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagella biosynthesis chaperone FlgN"
                     /protein_id="HGU4546352.1"
                     /translation="MTRLAEILDQMSAVLNDLKTVMDQEQQHLSMGQINGSQLQWITE
                     QKSSLLATLDYLEQLRKKEPNTANSVDISQRWQEITVKTQQLRQMNQHNGWLLEGQIE
                     RNQQALEMLKPHQEPTLYGANGQTSTTHRGGKKISI"
     gene            complement(39505..41040)
                     /gene="murJ"
                     /locus_tag="ACHOSC_RS01695"
                     /old_locus_tag="pgaptmp_000339"
     CDS             complement(39505..41040)
                     /gene="murJ"
                     /locus_tag="ACHOSC_RS01695"
                     /old_locus_tag="pgaptmp_000339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706990.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0034204 - lipid translocation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="murein biosynthesis integral membrane protein
                     MurJ"
                     /protein_id="HGU4546353.1"
                     /translation="MNLLKSLAAVSSMTMFSRVLGFARDAIVARIFGAGMATDAFFVA
                     FKLPNLLRRIFAEGAFSQAFVPILAEYKSKQGEDATRVFVSYVSGLLTLALAVVTVAG
                     MLAAPWVIMVTAPGFADTADKFALTSQLLKITFPYILLISLASLVGAILNTWNRFSIP
                     AFAPTLLNISMIGFALFAAPYFNPPVLALAWAVTVGGILQLVYQLPHLKKIGMLVLPR
                     INFHDAGTMRVVKQMGPAILGVSVSQISLIINTIFASFLASGSVSWMYYADRLMEFPS
                     GVLGVALGTILLPSLSKSFASGNHDEYNRLMDWGLRLCFLLALPSAVALGILSGPLTV
                     SLFQYGKFTAFDALMTQRALIAYSVGLIGLIVVKVLAPGFYSRQDIKTPVKIAIVTLI
                     LTQLMNLAFIGPLKHAGLSLSIGLAACLNASLLYWQLRKQKIFTPQPGWMAFLLRLVV
                     AVLVMSGVLLGMLHIMPEWSLGTMPWRLLRLMAVVLAGIAAYFAALAVLGFKVKEFAR
                     RTV"
     gene            complement(41150..42073)
                     /gene="yceM"
                     /locus_tag="ACHOSC_RS01700"
                     /old_locus_tag="pgaptmp_000340"
     CDS             complement(41150..42073)
                     /gene="yceM"
                     /locus_tag="ACHOSC_RS01700"
                     /old_locus_tag="pgaptmp_000340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HGU4546354.1"
                     /translation="MKKLRIGVVGLGGIAQKAWLPVLAAASDWTLQGAWSPTRAKALP
                     ICESWRIPYADSLSSLAASCDAVFVHSSTASHFDVVSTLLNAGVHVCVDKPLAENLRD
                     AERLVELAARKKLTLMVGFNRRFAPLYGELKTQLATAASLRMDKHRSNSVGPHDLYFT
                     LLDDYLHVVDTVLWLSGGKASLDGGTLLTNDAGEMLFAEHHFSAGPLQITTCMHRRAG
                     SQRETVQAVTDGALIDITDMREWREERGQGVVHKPIPGWQSTLEQRGFVGCARHFIEC
                     VQNQTVPQTAGEQAVLAQRIVDKIWRDAMSE"
     gene            complement(42075..42722)
                     /gene="yceH"
                     /locus_tag="ACHOSC_RS01705"
                     /old_locus_tag="pgaptmp_000341"
     CDS             complement(42075..42722)
                     /gene="yceH"
                     /locus_tag="ACHOSC_RS01705"
                     /old_locus_tag="pgaptmp_000341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YceH family protein"
                     /protein_id="HGU4546355.1"
                     /translation="MKYQLTALEARVIGCLLEKQVTTPEQYPLSVNGVVTACNQKTNR
                     EPVMNLSESEVQEQLDNLVKRHYLRTVSGFGNRVTKYEQRFCNSEFGDLKLSAAEVAL
                     ITTLLLRGAQTPGELRSRAARMYEFSDMAEVESTLEQLANREDGPFVVRLAREPGKRE
                     SRYMHLFSGEVEDQPAVTDMSNAVDGDLQARVEALEIEVAELKQRLDSLLAHLGD"
     gene            complement(42733..43317)
                     /gene="rimJ"
                     /locus_tag="ACHOSC_RS01710"
                     /old_locus_tag="pgaptmp_000342"
     CDS             complement(42733..43317)
                     /gene="rimJ"
                     /locus_tag="ACHOSC_RS01710"
                     /old_locus_tag="pgaptmp_000342"
                     /EC_number="2.3.1.267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal protein S5-alanine
                     N-acetyltransferase"
                     /protein_id="HGU4546356.1"
                     /translation="MFGYRSNVPKVRLTTDRLVVRLVHDRDAWRLADYYAENRHFLKP
                     WEPVRDESHCYPSGWQARLGMINEFHKQGSAFYFGLFDPDEKEIIGVANFSNVVRGSF
                     HACYLGYSIGQKWQGKGLMFEALTAAIRYMQRTQHIHRIMANYMPHNKRSGDLLARLG
                     FEKEGYAKDYLLIDGQWRDHVLTALTTPDWTPGR"
     gene            43553..44761
                     /gene="mdtH"
                     /locus_tag="ACHOSC_RS01715"
                     /old_locus_tag="pgaptmp_000343"
     CDS             43553..44761
                     /gene="mdtH"
                     /locus_tag="ACHOSC_RS01715"
                     /old_locus_tag="pgaptmp_000343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415583.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter MdtH"
                     /protein_id="HGU4546357.1"
                     /translation="MSRVSQARNLGKYFLLIDNMLVVLGFFVVFPLISIRFVDQMGWA
                     AVMVGIALGLRQFIQQGLGIFGGAIADRFGAKPMIVTGMLMRAAGFATMGIAHEPWLL
                     WFSCLLSGLGGTLFDPPRSALVVKLIRPQQRGRFFSLLMMQDSAGAVIGALLGSWLLQ
                     YDFRLVCATGAVLFVLCAAFNAWLLPAWKLSTVRTPVREGMTRVMRDKRFVTYVLTLA
                     GYYMLAVQVMLMLPIMVNDVAGAPSAVKWMYAIEACLSLTLLYPIARWSEKHFRLEHR
                     LMAGLLIMSLSMMPVGMVSGLQQLFTLICLFYIGSIIAEPARETLSASLADARARGSY
                     MGFSRLGLAIGGAIGYIGGGWLFDLGKSAHQPELPWMMLGIIGIFTFLALGWQFSQKR
                     AARRLLERDA"
     gene            44825..45472
                     /gene="grxB"
                     /locus_tag="ACHOSC_RS01720"
                     /old_locus_tag="pgaptmp_000344"
     CDS             44825..45472
                     /gene="grxB"
                     /locus_tag="ACHOSC_RS01720"
                     /old_locus_tag="pgaptmp_000344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004362 - glutathione-disulfide reductase
                     (NADPH) activity [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin 2"
                     /protein_id="HGU4546358.1"
                     /translation="MKLYIYDHCPYCLKARMIFGLKNIPVELHVLLNDDAETPTRMVG
                     QKQVPILQKDDSRYMPESMDIVHYVDKLDGKPLLTGKRSPAIEEWLRKVNGYANKLLL
                     PRFAKSAFDEFSTPAARKYFVDKKEASAGNLADLLAHSDGLIKNISDDLRALDKLIVK
                     PNAVNGELSEDDIQLFPLLRNLTLVAGINWPSRVADYRDNMAKQTQINLLSSMAI"
     gene            45606..46166
                     /gene="yceB"
                     /locus_tag="ACHOSC_RS01725"
                     /old_locus_tag="pgaptmp_000345"
     CDS             45606..46166
                     /gene="yceB"
                     /locus_tag="ACHOSC_RS01725"
                     /old_locus_tag="pgaptmp_000345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HGU4546359.1"
                     /translation="MNKFLFAAALIVSGLLVGCNQLTQYTITEQEINQSLAKHNNFSK
                     DIGLPGVADAHIVLTNLTSQIGREEPNKVTLTGDANLDMNSLFGSQKATMKLKLKALP
                     VFDKEKGAIFLKEMEVVDATVQPEKMQTVMQTLLPYLNQALRNYFNQQPAYVLREDGS
                     QGEAMAKKLAKGIEVKPGEIVIPFTD"
     gene            46272..47318
                     /gene="pyrC"
                     /locus_tag="ACHOSC_RS01730"
                     /old_locus_tag="pgaptmp_000346"
     CDS             46272..47318
                     /gene="pyrC"
                     /locus_tag="ACHOSC_RS01730"
                     /old_locus_tag="pgaptmp_000346"
                     /EC_number="3.5.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004151 - dihydroorotase activity
                     [Evidence IEA];
                     GO_process: GO:0019856 - pyrimidine nucleobase
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotase"
                     /protein_id="HGU4546360.1"
                     /translation="MTAPSQVLKIRRPDDWHLHLRDGDMLKTVVPYTSEIYGRAIVMP
                     NLAPPVTTVEAAVAYRQRILDAVPAGHNFTPLMTCYLTDSLDPNELERGFNEGVFTAA
                     KLYPANATTNSSHGVTSIDAIMPVLERMEKIGMPLLVHGEVTHADIDIFDREARFIES
                     VMEPLRQRLTALKVVFEHITTKDAADYVRDGNERLAATITPQHLMFNRNHMLVGGVRP
                     HLYCLPILKRNIHQQALRELVASGFNRVFLGTDSAPHARHRKESSCGCAGCFNAPTAL
                     GSYATVFEEMNALQHFEAFCSVNGPQFYGLPVNDTFIELVREEQQVAESIALTDDTLV
                     PFLAGETVRWSVKQ"
     gene            47392..47637
                     /gene="dinI"
                     /locus_tag="ACHOSC_RS01735"
                     /old_locus_tag="pgaptmp_000347"
     CDS             47392..47637
                     /gene="dinI"
                     /locus_tag="ACHOSC_RS01735"
                     /old_locus_tag="pgaptmp_000347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA damage-inducible protein I"
                     /protein_id="HGU4546361.1"
                     /translation="MRIEVTIAKTSPLPAGAIDALAGELSRRIQYAFPDNEGHVSVRY
                     AAANNLSVIGATKEDKQRISEILQETWESADDWFVSE"
BASE COUNT        11521 a        12901 c        11851 g        11367 t
ORIGIN      
        1 ctggtgaacg gaggtgatta tttttataat aacctttcat tcacagtcac caggtacaac
       61 ggcatcatgg caactgactc aacacaatgt gtaaaaaaaa gccgtggccg cccaaaagtg
      121 ttcgacaggg atgccgcgct tgataaggcc atgaaattgt tctggcaaca cggttatgaa
      181 gcgacttctc ttgcggacct cgtcgaagcg accggagcca aagcgcccac gctatacgcg
      241 gaatttacca acaaagaggg gttatttcgt gccgttctcg accgctatat cgatcgtttt
      301 gccgctaagc atgaagcaca gctgttttgt gaagagaaaa gcgtggagtc tgcgctggct
      361 gactattttg ctgccatcgc caactgcttt accagcaaag acaccccggc aggctgcttc
      421 atgatcaaca actgcaccac cctctcccca gattcaggag atatcgccaa tacgttgaaa
      481 tcacgccatg cgatgcaaga gcgcactttg cagcagtttt tatgtcaacg acaagcgcgc
      541 ggggaaatcc cgacccactg tgacgtgaca catctggcag aattccttaa ttgtattatt
      601 caggggatgt cgatcagcgc acgcgaaggt gcatcgctgg aaaaactaat gcagattgcc
      661 ggaacgactt tgcgtttatg gcccgaactg gtgaaataag tcgcaaaaaa agcccctcag
      721 cctgagcgaa tgaggggcca aattgcagag tacagccttg ttatactttg ttattcaaaa
      781 tcagttgccc attactatct agcgggatct gcgtacccgg atcgcggtcc atgcggattt
      841 tgccctgctg atcgccaatc ttataggtca catcataacc gagcattttt tctgacttgt
      901 catacaccgt tttacaacgc tgttgcgtag tcgtgtaagt atcgctttcc tggagagagc
      961 cctggatctg gttaccggca tatccaccac ccagcgcccc cacaacagtg gcgacatctt
     1021 taccgcgacc accgccaaac tgatgcccta tcacgccgcc agcaacagcg ccgagcaccg
     1081 acccggtaat gcgattttca tcctgcaccg gtcgacgatg ggtcactgtg acgttgcgac
     1141 actcctgacg cggtgtttta accgtttcct tgattggggt tgcagaaaca acctgagcgt
     1201 attgcgggcc acgttcaaac acgttcagac tggccactgc cgctacgccc agcgcagctg
     1261 cgacaccaat cccgataccc gccaacattg atttattcac gggaacacct ccttctttgc
     1321 agttatgcca atcatgcaag cgaaggagaa aagcagctat cagacagaag atgaaaagcg
     1381 cgggaatggc tggaaatact taatgattcg gaggagtctg aaggaatgcc tgatgcgacg
     1441 cttaacgcgt cttatcaggc ctaccgctcg taccatttgt ccagggcgga tgaggcgttt
     1501 atgccacatc cgccagtgta cgtcgattaa tgcaacttca aacgcggacg gataacgcgg
     1561 ttaatactcc ccaccagcat cattaatccg gttttaaagt aaccatgcag cgcaatctga
     1621 tgcattcggt atagcgagat atatacaaag cgcgcaattc gtccttcaat catcattgag
     1681 ccgcgcgtca ggttacccat caggctacca acggtggaga agttcgacag cgataccagc
     1741 gaaccatgat ctttatactg ataatttttc agcggcttac cattcatctg cgccagaatg
     1801 ttgttcattg cgcaagtcgc catctggtgt gcagcctgag cacgcggcgg aacaaagccc
     1861 ccttccggac gcgggcatga cgcgcagtcg ccaatagcgt aaatgtctgg atcgcgggtg
     1921 gtttgcagcg tcggttccac caccagctgg ttgatacggt tagtttcaag accaccgata
     1981 tctttcagga agtctggcgc tttgatcccg gctgcccaca ccatcagatc agcctcaata
     2041 tattcgccat ctttagtgtg caggccgcct tcatcagcac tggtgaccat ggtttgcgtc
     2101 agcacgcgaa cgccaagttt cgttagctcg ttgtgggccg cagcagagat acgtggcggt
     2161 aatgcaggca aaatacgttc tcccgcttct accagcgtta cgttcagggc ttcgttggtc
     2221 aggcctttgt aaccgtagct gtgcagttgc ttgaccgcgt tgtgcaattc agcggagagt
     2281 tctacacccg tcgcgccgcc gccgacaatc gcaatgttca ctttgccatt tgcgcccagg
     2341 ttggcggagt atttcaggaa gagattcagc atctcctggt gaaagcgacg cgcctggtgc
     2401 gggttatcga ggaaaatgca gttctctttg acacctggcg tattgaaatc gttagaggtg
     2461 ctacccagcg ccattaccag ggtgtcatag gcgattttac gttccggaac cagcagttca
     2521 cctttctcat cgcgcagttc tgcaatagtg attgttttcg cttcacgatc aatatcaatg
     2581 acggaaccca gctggaactg gaaaccatga ttgcgcgcat gggccagata gctcaacgca
     2641 tcgacgcctt catcaagcga gccagtcgcc acttcgtgca gcagcggttt ccacagatgg
     2701 ctgtggttac gatcgaccag cgtaattttg gcttttttct tgcgtcccag cttatgcccc
     2761 agctgtgttg ccatttccag cccaccagca ccgccgccga caatcacaat ctttttcaat
     2821 ggcgtagtca acgtgacccc cttaaattta ttaaccaatt gttaattaat ggttattaac
     2881 atagctctta attaacaaca ggttacagca atgaaaatgt tcttcgctat tgagaataac
     2941 atgaatggtg cattggtcat accaaaattg atgttaatca agttttgttg ctgaaaagat
     3001 aggcgacttt accgggtgag atcaaaaaac agagtttatt gtttattatt tgagcaagtc
     3061 cggaagggcg atatagggtg aagcgtatat cgagggcgtt tggtcgtcct gataagaggc
     3121 gcatcaggca ttgatgctcg aatgtcggat gcggcgtgaa cgccttatcc gacccacaga
     3181 atccggagcg catcaggtac cggcaaggtt acctgatgcg acgagggcat ttacccgagg
     3241 gttttgaacg ctttaatgcg ctgtaaatgc ggggagatat tcttgaattt gtgcgtctgc
     3301 tcttcgtccc agacaatctc gtaataatga tgcagctctt cagatgtccg ctggctgtta
     3361 agcgcttcat cattacgcga caaaatcacc aggcaacgat cgcgattctt ctcacggaag
     3421 ttggtcacac acttagtggc aatatcggca tactcttccg ggcgatcaat cttgccttcc
     3481 atgttctcat aagggaacaa attagggttg aagatcacct ggcggatatc gcaaagaaaa
     3541 ccaatccgtt ccgcccagta tccgcccaga ccaacgccgc aaatcagcgg acgctcgtcg
     3601 acgttcagtt gcaacatttt gtccacttct ttgagcagat gctgcatatc atgtttcgga
     3661 tgccgcgtac tgtagcttat caagcgtaca tccgggtcaa taaactgcaa ttgtaagact
     3721 ttctcgtggt tacccggact gttagagtca aaaccgtgta aatagataat catcgcttcc
     3781 tcacataagc cagggttagt gacctgcttt ctcttcctgc cagcgttcat gtaactgatt
     3841 cagacgggtg ctgatcgctt tccagcgagc cgagtccatc agttcctgtc gcgaaaatga
     3901 acctttatga tacaaacgtg taacacgttc tgccttgatc ggtgacagat tatctaacac
     3961 gctgacggcc cctttacgat tattgcagac caggatcata tcgcaacccg catccagtga
     4021 tgcctgcccg cgttcggcat aactgcccat aatcgcggca ccttccatcg ataaatcgtc
     4081 agagaaaatc acgccgtcaa aacccagttc ctgacgcaaa acggttttca gccagtaggg
     4141 agaaccgctc gccggacgcg gatcaacatc actgtagatc acatgcgcag gcataatggc
     4201 gtcgagttta ttttcgcgga ttaaggaact gaagaccgac atatctttag cgcgaatctc
     4261 cgcttgtgga cgtggatcgc acggtgtttc tttgtgtgag tctgccgtta ctgcaccgtg
     4321 tcctgggaag tgtttcccgg tcgttttcat tccggcttca tgcataccat caataaaccg
     4381 gctggcaatt gccagggctt tttgtggatc ggcatgataa gaacgctcgc caatcgccgc
     4441 gctgatatgc ccgacatcca gcactggcgc aaagctgata tcaatatcca tagcgatcat
     4501 ttcgctggcc atcagccaac ccgcctcttg cgccagtttg ccgccctctt ccattcctga
     4561 cagcgcagcg aatgattgcg ccgctggcaa gcgggtaaaa ccttcacgaa aacgctgcac
     4621 gcgtccacct tcctgatcta ccgccaccac cagatgattg cgcgaagctg cgcggatctg
     4681 gcgcaccagt tcacgcaact gggcaggatc atgatagtta cgcgtaaaga gaatcagccc
     4741 tcccaccagc ggatgcgcaa gtatttcacg ctcttccgcg tccagttcgt aaccttcgac
     4801 atccaacatt actggaccca cactgctctc cttattgttt tattaatagc tgccgccagg
     4861 tgtcatcggc cagcctgata aattgttgat cgccggtttg tcgccagcgg tactcaaacc
     4921 accctgcttt gagcatcagc agccagggaa accatcgcct gacctgacgc cataattgcg
     4981 ccggataaat cttcgcgcga gtggcatagt cattgaccaa ttgccggtgc tgttcagtat
     5041 tttccaccca cactgccgcc agttccagcg cgatatcacc atctccggca tactcccagt
     5101 cgatgagttt taaccctgac gcgctatgca ctaaatttcc ggcgtggaca tccatatgca
     5161 atggacttaa gcgtaaaggc cgtggttccc gcgctttgcg cagacgtttt aacattcgca
     5221 gccaacccac tgtccgccgc gccggatcgc tttgctgcca gtacagttcc agtaacggca
     5281 acagcgttat tcgccagcca aaacgtggtt gttgatgtag ataatacagc aagcctgcca
     5341 gttcgttggt atccggcaaa tacgttttta cctcgccggg cagatagtcg actaccatcc
     5401 agtcacggag atataaatgc ggcttcggtg caatgcttgc gggtagttgt gataaagccc
     5461 gatactggcg caagaacgcg gactgcggcg catcaggatc gtgcggctga cgcacaacaa
     5521 aacgctggcc ctgatgttca atgagaaaac tcccgccact aagcccacta ttaaacgtcg
     5581 tgacgggatg aaactgcggg aaaaagcgcg acagcaattc gtcgcgcatg atgggattat
     5641 tgctgcgaaa cggcaccttt acctgaccag ataatttcgc ccgtctgcac cagcatcagc
     5701 tgcatttgta gggtcggagc gttaacgttg ccagaggcgc tggagtacag cacgtaatga
     5761 gcgccgacat tgcgggcaat gcctatggct ttactacggg tacctaaact gtcctgcggc
     5821 gacaaaccta actgttgctt agccatcgac agctgctggg cggaaaccag ggtaaatttc
     5881 ccgttattag ccagcgcatt tcgcagcgtt tcggtcgctt ctgcggcatt cagcgaaccg
     5941 ttagtacggt tgttaacgct atcaaccagc aggacgctac ccgcagtcac cccgtcagcc
     6001 ccaagcatct tactgaccat cggctgcatt gcgccattcc agtcataatg gcgaatatgc
     6061 ggcgcaggcg gtgccgtttg atcttcgtgc tcaattgggc ctggctgctg cgggatcgtc
     6121 ggcaccgagg gcactgtggg gacaggctgt tgtggctcgg ctggttgttc cggcgctggt
     6181 ttcacttctt ctaccggtgc aggttcacgt tgccccacac acccggcgag aaacatcgcc
     6241 agcgcggtaa tcaaggcgta gcgactcatt tttgtcatca agattcaccc cttacaaata
     6301 tagataaagt ctgactttgt gcgcccccag aaaattggcg ctgccgtaca gcgttaccgc
     6361 cgaatgtgcg ggaatggtaa cgctgcgtgg cctttccaga ggatgcatct ccagccctct
     6421 ggcgtcatac cagtaaaaac gataatgaac ggtaacgggt tcttgccttt cgttataaag
     6481 cgttgaggat gctgaaggtt gaatatcaga cgtcgaaagg acgggctttt ctgcactgat
     6541 gcccgcagcc aataaagatg actccatcac cagcgattgc tcatcattca ccggaatttc
     6601 cggatgtgaa cgacagccca ccagcagtaa caacgccaga gacaccagcc caaagcatcc
     6661 ttttctcatc gttacagacc tttatgtgcc agcattggtc ccagcggacg gccacccaac
     6721 aagtgcatat ggatgtggta aacctcttgt ccgccatggc ggttggtatt catgatcaga
     6781 cgatagccat cttcggcaat accttcttgc tcagcaattt ttgccgctac ggtgatcatg
     6841 cgtcccagcg cctgctcatg ctcagctgac acgtcgttca cagtcggaat gaggatattc
     6901 ggaatgatca gaatatgcgt tggcgcttgc ggcgaaatat cgcgaaacgc cgttaccaga
     6961 tcatcctggt agacgatatc ggaggggatc tcacgacgaa taattttgct gaatatagtt
     7021 tcttctgcca cgacattttc ctttttcaaa tagccatgcg ttgcgccatg ctacgccgcg
     7081 ccacactgcg agtatagagt atggtcgagt taaacctgct ctttcaacct taactcacga
     7141 tttcttaagc aaaaaatcgt tttgctgccg tatatatcgc cattattccc atttctgcct
     7201 gcgaatgcgc tgaaaagaca ttctctgttt ggtttacacg tcaaatatgg atgcgtatat
     7261 ttctcatttg catttacata caaaattatt cgcacactaa atataaccgc tcatcaccct
     7321 cctccggcat gagcctgacg acaacataaa ccaagagatt tcaaatgctt tcaacacaat
     7381 ttaacaggga taatcaatat caagccagca ccaaaccatc actacttgcc ggttgcatag
     7441 cattggcact attaccttct gccgcttttg ccgctccagc cactgaagaa acggtgattg
     7501 ttgagggttc agccacagct ccagatgatg gcgaaaatga ttacagcgta acgtctacct
     7561 ctgcgggtac caaaatgcag atgactcaac gtgatattcc tcagtcggtc actattgtta
     7621 gccagcagcg gatggaagat cagcagttac aaacgctggg cgaagtgatg gaaaacacgc
     7681 tggggatcag caaaagtcag gcggattccg atcgtgctct ttattattcc cgcggattcc
     7741 agatcgataa ctatatggtt gatggtatcc ccacctattt tgaatcgcgc tggaatctgg
     7801 gcgacgcact ttctgatatg gcactctttg aacgcgtaga agtagtgcgt ggcgcgacag
     7861 gactcatgac cgggacgggt aatccatctg cggcaattaa tatggttcga aaacacgcga
     7921 ccagtcgtga atttaaaggc gatgtctcgg cggaatacgg tagctggaac aaagaacggt
     7981 atgtggcgga tttacaaagc ccactcaccg aagacggtaa aatccgcgcg cgaattgtcg
     8041 gcggctacca gaataacgac tcatggctgg accgctacaa cagtgaaaag accttcttct
     8101 ccggcattgt cgatgctgat ttaggcgatc ttacgacgct ttcagccggt tacgaatatc
     8161 agcacattga tgttaatagc ccaacctggg gcggtttacc gcgctggaat actgatggca
     8221 gcagcaacag ttacgatcgc gcacgcagta ccgcacctga ctgggcgtac aacgataaag
     8281 agatcaacaa ggtctttatg accctgaagc agcggtttgc tgatacctgg caagcgacgc
     8341 tgaatgccac ccactctgaa gtcgaatttg acagcaaaat gatgtatgtc gatgcctatg
     8401 taaacaaagc ggatggtatg ctgattgggc catacagtaa ttatggacct ggctttgatt
     8461 atgtcggcgg caccggttgg aacagtggca aacgtaaagt tgatgcgctg gatttgttcg
     8521 ctgacggtag ttatgaattg tttggtcgtc agcacaatct aatgtttggt ggcagttaca
     8581 gcaaacaaaa caatcgttac ttcagttcat gggccaacat cttcccggat gaaattggca
     8641 gtttctacaa ctttaatggc aatttcccac aaaccgactg gtcaccacag agcctggcgc
     8701 aggacgatac cacacatatt aaatcgttat atgctgccac tcgtgtcacc cttgccgatc
     8761 cgctgcatct gatcctcggc gcacgttata ccaactggcg ggttgatacg ctgacttaca
     8821 gcatggagaa aaaccacacc acgccttacg ctggtctggt gtttgacatc aatgacaact
     8881 ggtcgaccta cgccagctat acctctattt tccagccgca aaatgatcgt gacagttcag
     8941 gcaaatatct ggctccaatc accggtaaca actacgagct gggtctgaaa tcggactgga
     9001 tgaatagccg tctgaccacc acgttagcca tcttccgtat tgagcaggat aatgtcgctc
     9061 agtccaccgg tacacctatc cccggcagca acggcgaaac cgcctataaa gcagtggatg
     9121 ggacagtcag taaaggggtg gaatttgaac tcaacggcgc aattaccgac aactggcagc
     9181 tgacatttgg cgcaacgcgc tatattgcag aggataacga aggaaacgcc gttaatccta
     9241 atctgccacg caccacggtt aaaatgttca ccagctatcg gttgcctgtc atgccagagt
     9301 tgacagtcgg cggtggtgtt aactggcaaa atcgcgtgta taccgacacc gtgacaccgt
     9361 atggcacctt ccgcgccgag caaggtagct acgcgctggt ggatctcttc acccgctacc
     9421 aggtgacgaa aaacttctcg ttacagggga acgtcaataa cctgttcgac aaaacctacg
     9481 ataccaacgt ggaaggttct atcgtctacg gcgcaccgcg taatttcagc attaccggca
     9541 cgtatcaatt ctgaatagca cctgtaaaaa aggcagccat ctggctgcct tagtctcccc
     9601 aacgtcttac agattagtgg ttacggatgt actcatccat ctcggttttc aggttgtcgg
     9661 atttagtacc gaaaatcgcc tgaacaccag aaccagcaac cactacgccc gctgcgccca
     9721 gtttcttcag gccagcctga tccactttag acacatcagc aacgctgacg cgcagacggg
     9781 taatacatgc gtcgaggtta gtaatgtttt ctttaccacc aaatgcagca accagagccg
     9841 gtgccatttc gctggtacct gtcgcttttg catcttcagt cgcgtcttca cgacccggcg
     9901 ttttcagatc cagtgcttta atcagcacgc ggaagatggt gtagtaaaca atcgcataac
     9961 cgataccgac gatcgggaac agccacagtt tgctgctgtt accagacaga acgatgaagt
    10021 cgatcagacc gtgcgagaac gacgtaccgt cacgcatccc cagaagaata cagattggga
    10081 atgccaggcc tgccagaatc gcgtggatga tgtacaggat cggcgcaacg aacatgaagg
    10141 agaactcgat cggctcggtg ataccggtca ggaacgaggt cagcgccgcg gagatcataa
    10201 taccgcccac tttcgcgcgg ttttctggtt tagcagagtg ccagatagca attgcggcag
    10261 ctggcagacc gtacattttg aacaggaagc caccagacag tttacccgca gtcgggtcac
    10321 ccgccatata acgcggaatg tcgccgtgga aaacctgacc tgctgcgttg gtgtattcac
    10381 caatctgcat ctggaaaggt acgttccaga tgtggtgcag accaaacggt accaggcaac
    10441 gttcgatgaa accgtaaatg ccaaacgcaa ctaccgggtt ctggtaagca gcccactgag
    10501 agaaggtctg gattgcagaa ccaatcggcg gccaaatgaa ggacagcaca acgccagtaa
    10561 agatggcagc caggccagaa atgatcggca caaagcgttt accggcaaag aagccaagat
    10621 actcaggcag cttaatacgg tagaaacggt taaacatgta cgctgcgatc gcaccggaga
    10681 taatccctcc gagtacgcca gtatccgcca ggtgtttaga ggcgatttct tcagcaggta
    10741 aatgcagtac cagtggcgca accacggcca tggttttaac catgatgcca taggcaacaa
    10801 ctgcggccag cgcggatacg ccatcgttat tggtaaagcc gagggcgaca ccgatcgcaa
    10861 aaatcagtgg catgtttgca aagacggaac cgcctgcttc tgccataaca tgcgatacaa
    10921 cggcgggcag ccagctgaaa ttcgcggaac cgacgcccag cagaatacct gcgataggca
    10981 gtacggatac cggcagcatc agcgatttac cgaccttttg caggttagca aatgcattct
    11041 taaacataat tgagagtgct cctgagtatg ggtgcttttt ttacgttctc acgcgtggca
    11101 aggggggaga gcctcgccgt gtacagggca tctaagcgcc ctttatttat tacacagagt
    11161 aaaataattc agtgccaatt tgtttgacgg ctatcacgtt tcaacttcag atcattggag
    11221 aagttgcaca ttttgacaaa acatatgtca caatatttca aacgtggtta ttcaccgccc
    11281 aattttcggc ggtgaacttt actcgtttta gccattaatt acgagcttta aaaaaactca
    11341 ttcaacggat ggattgaagg cgggaagcgt cgatgtgaaa cagacgggcg aagttatcgg
    11401 tggttacctg cgccagttct tcaacggcaa cacctttcaa cacagccatg tattctgcaa
    11461 cgtcacgaac catcgcaggt tgattctctt ttcctcgatg cggtaccggc gcaaggtaag
    11521 gtgagtcagt ttccaccagt aatcgatcca gggggacata acgcgcagca tcgcgcagtt
    11581 gctccgcatt acggaaggtc acaatgccgg aaaaggagat gtaaaatccg agatccagta
    11641 atttacccgc cgtttctctg tcctctgtaa aacagtgtag tacgccaccg caatccgtca
    11701 ctttttcttc gcgcagaatt gccagcgtat cggcacgggc gtcacgggta tggacgataa
    11761 ccggcttgtt cagttcacga ccaatctgga tatgatggat gaaggactct tgctgacgta
    11821 ctttagtttc cggtgtgtaa taataatcca gcccggtttc acccagcgct acaacaccct
    11881 cttctgccgc cagacggcgt aaatcttcta catcgtaggg atcattctgg ttgagtggat
    11941 gcacgccaca agaaaatacg acgttgtcac gttcgcctac cagatcccgc atatgtaaat
    12001 aacccggtaa tgttgtggcg actgccagac aaaatttcac atcgcgtgcg gcggctttcg
    12061 ccagaacgtc atccacgtcc ttatgcaaag attcataatc cagaccatcg agatggcagt
    12121 gtgagtcgac taaaaacatg atgtctctct taaagatgag gaaccggtag cacaacgccc
    12181 ggttgcaggt aatgctcaat acgcagtaaa agatcggtga tgagaagctc gcggttgatg
    12241 cctgtaacag acattaactg ttcacgaatg tggcaaacat cccccagtat agcctgcagg
    12301 cgcgagggag aaagatggtt tgccagttcg gcgaccaggc ccggcacatc aacattggtc
    12361 acctgcgcag caccatgatg gcgttttagc gcatccatca gcaacgttgc cagccagtgt
    12421 aaacgcgccg gagcttgttc atgattaagg gccgctagca gcgaatacca gtcgcccgat
    12481 ggcacgctat atgccaacgc ctgacacaat gtttcacgag cctgccagtt atctccctga
    12541 aacaacgcca gtgccgcgcc aggcgaaccg gcgcttaagc gcaatgcggc aagtaatgca
    12601 tcctgtgaca ttgtcacttc gcgtgaaagc caggtcacgg cgtactgttc cggcggcggc
    12661 gcaaggtaat gtaaccgaca acgactacgt aatgttgcca gtaaacgttc aggctcgcgg
    12721 gtagccagga aaaaccaggt ttctgctggt ggctcttcaa gcgttttcag caatgcgtta
    12781 gccgcggcgt cggtcagtaa ggcagcatcg gttacccaga caactttcgc accacctagg
    12841 cgtgcgtgct cattcagctt ttcggtgacc tcacgtaccg catcaatgcc cagcatattt
    12901 ttcccttttt cgggagccag ggtgtagtaa tcgggatgcg tgccagcctg catcaactga
    12961 catccacgac agtgaccgca acttttgtgg ccctgcggtt gttggcagag taaataacgg
    13021 ctcagggcgt agattaaagc atcatcgccc atgcccggta acgcctgaat gagtagcgca
    13081 tggtgacctc ttccggcctg atagctggct accagttttt cgaaatcagg tcgtaaccat
    13141 ggataccatc tcatgcgtcc aactccttaa cccagtgggt cacggtagtg cggattgcat
    13201 ccatcacggc ctccagcggc tgggtggcat caatggtatg aatactttta tcttgtgcag
    13261 ccagttccag atagcgggcg cgggtgcgat taaagaaatc gaaagactct tgctcaatac
    13321 gatccagctc gccgcgcgca cgcgcgcgtt ttaagccaac ttccggggta acatcgagat
    13381 agagcgttaa gtcggggcga aaatccccga gaacagcatc acgcagtgtt gccagcatat
    13441 gttggtcaat accacgtcct ccgccctgat acgcctgagt ggagagatcg tggcgatcgc
    13501 caatcaccca ggtgccgtta gccagagctg gtttgatgac cgtttctacc agttgaacgc
    13561 gcgcggcata aaacatcaga acttcggctt tatcggtaat gacttcatcg cctaccgatt
    13621 tgatatccag caccaggctt cttaactttt cggcaagttg cgtaccgcca ggttcccgag
    13681 tgaaaaccat gtcgcggata cccagttgct caagcgtctc aaccaccaca ttacgcgcgg
    13741 tagttttgcc tgcgccttcc agcccctcaa tgacgatata cttactgcgc atttttttcc
    13801 ttaagcactt tcagataatc ctgcacagac ttgttatggc tggcaagatt ggtattaaac
    13861 gtgtgaccac ctttaccatc ggccacaaaa tagagatacg gcgtttttgc cggatgcgca
    13921 gcagccttca gcgaatccgc ccccggcgta gctatcgcac ccggcggcaa accggtaatg
    13981 gtataggtgt tatacgctgt cggcgtttcc aggtctgcac gagaaagttt gccattataa
    14041 cgctctccca tcccgtaaat cacggttggg tcggtctgca ggcgcatacc aatgcgtaaa
    14101 cggttgataa ataccgaggc aacctgatcg cgttcactgg caacggcggt ttctttttcg
    14161 ataattgatg ccatcgtcac cagctgattt ttatctttat aaggcagacc gtccgcacgc
    14221 ccttcccagg cgctatcgac cgctttcacc attttcttgt gcgctcgctt gagtaacgcg
    14281 acatcggtgg tattggcggt atacatccag gtgtctggcc agaaccaacc ttcaatccac
    14341 tccgggtttt ccagttcaag tgcctgcgct acggtggcgt acttatcgtc actcagcgtg
    14401 tgcttgatat acggagcctc acgcaattgc ttgaggtagt cgctcagacg catcccttct
    14461 accagtcgca gcgggaactg tgcttcttta ccgctttcca acaatttcag catctcgcgc
    14521 acggtcatct gcggtgtaaa gcggtaagtc ccggctttaa agtgagaaag atccggttcg
    14581 atacgcagca gccattgaaa aacccgtggg cgattgatga tcttatcagc ataaagctgt
    14641 tcaccgagcg ccagacgtcc ggtccctggc ttcagggtaa atatcgtctc ttctttgata
    14701 agcaatttgc tgtcggcaag atggcgaacc ttccagacgc ccacaccagc ggcgataccc
    14761 agtaccacca gcaataacaa gataatcaat aacacttttt tcatgactaa ttcgggcgct
    14821 cacaaagtgg ggctaaatat tcatataacg ttgctgacga aaaggagaca tcgccacagg
    14881 cacgtacggg catcactggc attaacgcat tacaaataac catctcatct gcctgcaacg
    14941 cctcttccag agaggcttgc acttcgacaa gctgataaga ggattgtgcc agcaaacgga
    15001 tacagaattg tcgcataatg ccgttaacac ctgcctgatc cagtcgcggc gtataaacta
    15061 cattgccctt ccgccagaac aaattagccg cacagcattc cgtaacccac ccttcgctgt
    15121 caaggaccag cgcctcatca gcgtttgtct gctcaagatg agagcggatc aatacttgct
    15181 caagccgatt aagatgttta atacctgcaa gatgaggatt gcgccccagc cgcaccgggc
    15241 ttagcgccaa cgtcatccct tcgttacgca aacggtcgta atgtgcagga taaaccgtaa
    15301 cggagagaat ccgcgttgcc ggtccactgt tcaatgtgct gtaccctcgc ccgccactac
    15361 cgcgactgat cacgactttc agcacaccat tttgctgttc tgctgccagc gttttcatct
    15421 cttgttcaag ctgaggccag aagtcacaga aaatcatcaa ccgctgacaa gcatcctgta
    15481 gtcgctggat atgcgccgat aacaaactga ctttaccgtc gataactctg gcggtggtaa
    15541 aacaaccatc accaaactgc gttgcccgat cgcttactgc cagcgattcc tgcttataac
    15601 cgttaattaa gaacataccg gctccttatc agagaattgt tagtgtggca gcatgttcac
    15661 tacggaacaa gtcggaataa aagctaagaa aaaaggcccg caagcggacc ttttatatgg
    15721 gtggaaaatg acaacttaga tctttttaaa gatcaaagaa ccattagtgc caccgaagcc
    15781 gaaggagtta cacagcgtgt attccattcc gctgacctga cgtgcttcat gcggtacgaa
    15841 atccagatcg caaccttcat ccgggttatc caggttgatg gttggcggaa cagcctgatc
    15901 gcgcagcgcc aggatggagt agatagattc tactgcaccc gccgcaccta acaggtgacc
    15961 ggtcatagat ttcgtggagc ttaccaacac acggcttgca gcttcaccga agatggtttt
    16021 caccgcctgc gcttcagctt tatcgccagc cggcgtagaa gtaccgtgcg cgttaacgta
    16081 gccaatctga ctcgcttcaa tgcctgcatc acgcagagca tttgccattg ccagagctgc
    16141 gcctgcgcca ttttctggcg gtgacgtcat atgataagca tcgctgctca taccaaagcc
    16201 gacgagttca gcgtaaattt tcgcaccgcg ttttttcgcg tgttcgtact cttcaagtac
    16261 cagcataccg gcaccatcgc ccagtacgaa gccatcacgc tctttatccc acgggcggct
    16321 cgccgcttgc gggttatcat tgcgggtaga taatgcacgt gccgcgccaa aaccaccaac
    16381 gcccagcggc gtactggctt tctctgcgcc acctgcaacc atcacgtcag catcgccata
    16441 cgcgataata cgcgcagcat gaccaatgtt gtgcacgccg gaagtacagg cggtcgcgat
    16501 agagatgctt gggccacgca ggccatacat gatagtcaga tgacctgcca ccatgttcac
    16561 aatcgttgac ggaacgaaga atgggctgat cttacgtgga ccaccgttca tcagagatgt
    16621 gtggttttct tcgatcagtc cgaggccgcc aatcccggag ccaattgcgg caccaatgcg
    16681 ggttgcgttc tcttccgtta tttcaaggcc agaatcctgc atggcctgaa cgccagcgac
    16741 aattccatat tgaatgaagg catccatctt gcgctgttct ttgcgcgaga taatgtcctc
    16801 acagttaaaa tcctttacta agccagcaaa tttcgttgca taggcgctag tatcgaaatg
    16861 gtcgattagg ctgatgccac tctgaccggc aagcagagct ttccaggtag actctacggt
    16921 attgccgaca ggagacaaca tgcccagtcc ggtcacaact acacgacgct tagacacgtt
    16981 tgtcctccag ggagggaaaa aatgattcta gtgggacaaa aagataaaac tcaggcggtc
    17041 gaacgaccgc ctggagatgt tcacttacgc ctggtggccg ttgatgtaat caatggcagc
    17101 ctgaacggtg gtgattttct cagcttcttc gtccggaatc tcagtatcaa actcttcttc
    17161 cagagccatt accagctcaa cggtgtcaag agaatccgcg cccaggtctt caacgaaaga
    17221 agcattgttg gtaacttctt cctgcttaac gcccagctgt tcgccgataa ttttcttaac
    17281 gcgttcttcg atagtgctca tactcttaaa tttcctatca aaactcgctt tcgcgatggt
    17341 tttcgtagtg tataaaatgt tgaaaaattt gcaactaaat cccggcaggt cttaccacga
    17401 ttttacgtta ttttgaggcc ttacccccca ataacgcaaa tcattttgca ctaattatta
    17461 ctcataacca cgcagactgc gcgcaacatg agcattttgt gcaaatcgcg gtcagaccat
    17521 gtacatcccg ccgttcacat gcaaagtttc acccgtgatg taagctgctt cgtcggatgc
    17581 caggaatgca accgcgttgg cgatttcctg tgcgccgccg aggcgacccg caggaacctg
    17641 cgccaggata cccgcacgct ggtcatcgct cagcgcacgt gtcatgtccg tttcaataaa
    17701 gcccggagca acaacgttta cagtaatacc gcgtgacgca acttcgcgcg ccagtgattt
    17761 actgaagccg atcaagcccg ctttcgccgc agcgtagttg gcctgaccgc catttcccat
    17821 ggtaccaacc acagaaccga tagtgataat acgaccatga cgctttttca tcatagcgcg
    17881 cattaccgct tttgacagac ggaaaacaga tgaaaggttg gtttcgataa tttcgttcca
    17941 ctcttcatct ttcattcgca ttaacaggtt atcacgagtg ataccggcat tattgaccag
    18001 gatatccact tcaccaaatt ctgcgcgaat tttttccaga acagattcga tagatgccgg
    18061 gtcggtcaca ttcaacatca gacctttgcc gttggcacct aaataatcgc tgatcgcctg
    18121 agcgccattt tcactggtcg cagtgccaat aactttcgcg ccacgggctg cgagcgtttc
    18181 agcaattgcg cggccaattc cgcggcttgc accggttacc agtgcgattt ttccttcaaa
    18241 attcatgatt ttcctctttt aaagctcgag cgccgctgcc atcgctgaag gttcgttcag
    18301 cgccgaggcg gtcagggtgt cgacaatgcg tttcgtcagg ccagtaagca ccttgcccgg
    18361 gccgacttca tagagatgtt ctacgccttg cgctgccatg tactcaacag acttcgtcca
    18421 ctgaaccggg ttatacaact gacgtaccag tgcgtcacgg atggcatcac cattggtttc
    18481 gcatttcaca tcaacgttat tcacaacaga aactgttggt gcgttaaagg tgattttcgc
    18541 taattctact gccagtttgt cggctgctgg tttcatcagc gcacagtgag acggtacgct
    18601 cactggtaac ggcagcgcgc gttttgcgcc agcagcttta caggcagcgc cagcacgctc
    18661 aaccgcttct ttatgaccgg caataaccac ctgtcccgga gagttaaagt ttaccggaga
    18721 aacgacctga ccttctgcag cttcttcaca cgctttcgca atagacgcat catccagacc
    18781 gatgattgcc gccatagcgc cagtgccttc cggtacggct tcttgcatga acttaccgcg
    18841 catctcaacc agacgcaccg catcagcgaa attaatcacg ccagcgcaaa ccagggcgga
    18901 gtattccccc aggctgtgac cggccatcat tgccggtgct ttaccgccct gctgctgcca
    18961 tacgcgatac agcgcaacag atgcaatcaa cagcgctggc tgggtttgcc aggttttatt
    19021 cagttcttca gctggcccct gctgggtcag cgcccacagg tcgtagccca gcgccgcaga
    19081 agcttcagca aacgtttctt cgacaattgg atagctcgcc gccatatcag ccagcattcc
    19141 aacggtttga gaaccctgtc cagggaacac aaatgcaaat tgcgtcatgt tttaatcctt
    19201 atcctagaaa cgaaccagcg cggagcccca ggtgaatcca ccgccaaagg cttcaagcag
    19261 aaccaactgc cccggcttaa tgcgcccgtc gcgtacagct tcatccagcg cgcacgggac
    19321 agaggccgca gaggtattac cgtggcgatc cagcgtcacc acgacattgt ccatagacat
    19381 accgagtttt ttcgccgttg cactgataat acgcaggtta gcctgatgcg gaaccagcca
    19441 gtccagttga gaacggtcaa gattatttgc cgccagcgtc tcatcaacga tgtgcgccag
    19501 ttccgttacc gcaaccttga agacttcgtt gcccgccatc gtcagatgaa ttgaattctc
    19561 tggattcaca cggtcagcat taggcagcgt cagcaactca ccatagctac cgtcggcatg
    19621 cagatgggtg gagatgattc ccggctcttc agaggcagcc agcaccgcag cgcccgcgcc
    19681 atcgccaaaa ataataatag tcccacgatc ggttggatcg caggtgcgcg ccagtacatc
    19741 ggaaccgacg accagagcat acttcaccgc cccagatttc acgtattgat cggctacgct
    19801 taatgcatag gtgaaacctg cgcaggctgc tgcaacgtca aatgccgggc aacctttaat
    19861 gcccaacatg ctttgaatct gacaagctgc gctcgggaaa gcgtgcgtag cagaagtcgt
    19921 tgccacaacg atcaggccaa tctggtcttt ctcaatgccc gccatctcaa ttgcgcgtgt
    19981 cgccgcttca aagcccatgg ttgaaacggt ttcgtttggc gcggcaatgt ggcgttcgcg
    20041 gataccggta cgagtgacaa tccactcgtc agaggtgtcc accatttttt ccaaatcggc
    20101 gtttgtccgc acttgttcgg gcagatagct gccagtacca ataatcttcg tatacatgta
    20161 cgctcagtca cttttcggtt atataccgtc acttgcaaac tgcgagttcg ctggcagcgt
    20221 cctgctaccg cagagttccg cttttgccac cgtccagcag ctcaaaacca gctgggtata
    20281 cagattccag gcgagcggca attcgctgag gaacttgtcg ctgcaccgcc tgcactgcct
    20341 gttcaatcgc gaccgcaaaa gctcgctgat tggctgcacc atgacttttt atcaccgtgc
    20401 cgcgcaatcc taacagacag gcgccgttat actggtcggg gttgaggtga ctgaatcgcc
    20461 tcgtcaggct cttttgtagc caacgcttta ataacagtag ccaccacgac cgttttttcc
    20521 cttcaccctg agatttcagc agagaaagga acatcctgac aacaccttcc atcgtcttta
    20581 acgtgacatt tcctgtaaag ccgtcacaaa ccagcacatc tgtcttgcca gttaacaact
    20641 cattggcttc aagatagccg atataattga tagaagggat tgttttaagc accgctgagg
    20701 catcccgaat actgtcgaga ccctttactt cttcttcacc aatattgagc aacgccacgc
    20761 gaggattggg aatttccacc acctcttcag ccagaactga gcccataatg gcaaattgca
    20821 ccaacattgt gctgtcacaa tcgacgttgg cccctaaatc gaggaccacc gttttgccct
    20881 tttgctgatg tggtaatacc gtcaccagcg ccggacgctc aatcccctcc aggggcttga
    20941 gtaataattt tgccagcccc atcagtgccc cggtattacc ggcactgaca caggcttgcg
    21001 ctcgaccttc tttcaccagc tccagggcca tacgcattga actcccacga ctggcgcgga
    21061 tagcttgcga aggccgggca tcactggcga taactgactg cgcaggaata atctgcagac
    21121 gcgaacgttg ttcaaagtca gctttagcaa gtaatggcgt gatggcgtcg ggattgccga
    21181 ctaaaagaag agtgagttgc gaattagaat tcagtgcctg caatgctgca ggcactgtca
    21241 cggaagggcc aaaatcccct cccatgacat ctaacgccag ggttagacgt gtcaaggtat
    21301 cgtcgccgcc cggtttggtc tttccccagt tgcctgggga aaatcctcac taagcttcat
    21361 cacgcagacg cgtgattact tagcgatgac cttgcggccg cggtagtaac cgtcggcagt
    21421 gatgtggtga cgcaggtgtt tttcaccaga agttttgtct acagacaggc tggtgactgc
    21481 ggtcagcgcg tcatgggaac gacgcatgcc acgtttggaa cgggttggtt tattctgttg
    21541 tacggccatg gaccttactc ctcaattacg gtttatcccc gatccaacgg ggagcaccaa
    21601 ttacttacgc tttaagctgg ctaatacggc aaatgggttt ggcttttgcg cttcttcagg
    21661 cagttcacca aagaccatgt ccgcttcgga cacttcacag tgttcagaat catgcaccgg
    21721 aactaccggc aaggcgagga tgatttcatc ttcaaccatt gcaagcagat cgatttcacc
    21781 gaattcgtta acctcaatcg gttcatacgc ttccggcagt gcttcagcct gttcgtctga
    21841 acgcacagga ctaaaacaat acgttgtgta gacctgatga gtaaacggct tcccgcaacg
    21901 ctgacactcg agcgttaccg tcaccttcgc atcgccgttt aacactgcga gacgttggtt
    21961 atcgatagcg aacgacatgg agcattccac atcactgtcc acactgacta cggattcggc
    22021 gacgcgctca acctgatcag gggtatagat accctggtaa tcaaggcgtt tttgagccgt
    22081 acgaaccgga tcgagagtca ggggtaattt taccttttgc atagggcgcg catattaact
    22141 ttgtaacgtc atagagtcaa agaaaaaggc agcctgtggt tgccttttgc caataattcg
    22201 cacacattgc gggttactgc tttattttcg ttcagtgctg ataacgaaca tcaacctgca
    22261 aaacgaagcg catagtttaa aatggctatc atcaatacgc tatatctgat ggaaaaaaat
    22321 atgcctaaac ttattttagc ctccacctcg ccatggcgtc gcgctcttct ggaaaaactg
    22381 caaatctctt ttgaatgtgc agcaccagag gtcgacgaaa ccccacgcag cgacgaatca
    22441 ccgcgacagt tggtgcttcg actggcacaa gaaaaagcgc aatctctggc gtcacgttat
    22501 ccggatcatt taattattgg ttcagaccag gtatgtgttc ttgatggcga aatcaccggt
    22561 aaaccgttaa cggaagaaaa tgcccgtctg caattacgca aagccagcgg caatatcgtc
    22621 accttctata ctggactggc gttgtttaat tcggcgaatg ggcatctaca aacagaagtg
    22681 gagccttttg acgtccattt ccgtcacctg agcgaggcgg agattgataa ttacgtgcgt
    22741 aaggagcatc ccctgcactg cgcgggtagc tttaagagtg aaggatttgg cattacgctg
    22801 tttgagcgtt tagagggacg tgaccctaac acgctggttg gtttaccgct aatcgcgctg
    22861 tgtcagatgt tacgtcggga agggaaaaac ccgctgatgg gatagttgtt tgcgccggat
    22921 gcggttacgt cgcatccggc aatcggtgca ggcggcctga tgcggcgtaa acgccttatc
    22981 aggccaaccg cccattatca ggcttatatg cttgtattag cgcgcgttac gcagcttttg
    23041 caaacaacgc ttcaaacctt catccatcgg cgcttcgata cgcatcacct caccggtccc
    23101 cggatgggta aacttcaacg ctgcggcgtg caggaacaga cgatttaatc ccgtgcctgc
    23161 ttcagtgagc tgtctgtcaa attcacggtc accgtagcga tcgtcaaagg caatcggatg
    23221 acccgcatac tgtgtatgca cacggatctg atgagtacgt ccggttaccg ggctacaacg
    23281 caccagggtg gcaaatgcat agcgttcttc cactttaaag cgtgtttccg acggtttgcc
    23341 ttcctgactg acacgcacga tacgttcgcc gctttgcaga atatttttca ataacggcgc
    23401 ttgaacgctc ttcacatgcg actgccactg accgcgcacc agcgccaggt aatctttttg
    23461 cattcctttt tcacgtaatt gctcatgcag agaacgcaac gccgagcgtt ttttcgctac
    23521 cagcaacaca cctgaagtgt cccggtcgag acgatgaacc agttcaagga accgcgcttc
    23581 cgggcgcaac gcccgcaaac cttcgataac gccgaagctt aaaccactgc cgccatgtac
    23641 cgccgtaccg gaaggtttat tcagcaccag gatatgatca tcttcatata agatgacgtc
    23701 cgccagcgcc gccacctttt gcagatgtgg cgaaaccgcc tcttcttccc gctcagcaac
    23761 gcgaaccggt gggatacgca cctcatcacc cgcttcgagt ttatattcag gcttaatacg
    23821 ttttttgttc acccgcactt cacctttacg caaaatacgg taaatcatac tttttggtac
    23881 gcctttcaat tgggtacgca aaaagttatc gatacgttgc cccgcttcgt cagcggtgat
    23941 agcaacaatt tttacggatg gagtctctgt tttcatggtg ggcgattcta attagccaac
    24001 aggattcgcg ccactcattt ttctatgctt atatttagtt ttacacctta ttacttcact
    24061 acgtgatcac tttattgatg gttattaaac caatcaccag caagaagtga aaaaactgtg
    24121 agtaagcggg tgataaatgg taaaagtcat cttgctataa caaggcttgc agtggaataa
    24181 tgaggccgtt tccgtgtcca tccttgttaa aacaagaaat tttacggaat aacccatttt
    24241 gcccgaccga tcatccacgc agcaatggcg taagacgtat tgatctttca ggcagttagc
    24301 gggctgcggg ttgcagtcct taccggtaga tggaaatatt tctggagagt aatacccagt
    24361 ctgtttcttt gataattgcg ctgtttttcc gcatgaaaaa cgggcaaccg acactctgcg
    24421 cctctttgag ctgacgataa ccgtgaggtt ggcgacgcga ctagacacga ggccatcggt
    24481 tcacacccgg aaaggcgtta ctttgcccgc agcttagtcg tcaatgtaag aataatgagt
    24541 aagttacgat gaaaagaatg ttaatcaacg caactcagca ggaagagttg cgcgttgccc
    24601 ttgtagatgg gcagcgtctg tatgacctgg atatcgaaag tccagggcac gagcagaaaa
    24661 aggcaaacat ctacaaaggt aaaatcaccc gcattgaacc gagtctggaa gctgcttttg
    24721 ttgattacgg cgctgaacgt cacggtttcc tcccactaaa agaaattgcc cgcgaatatt
    24781 tccctgctaa ctacagtgct catggtcgtc ccaacattaa agatgtgttg cgtgaaggtc
    24841 aggaagtcat tgttcagatc gataaagaag agcgcggcaa caaaggcgcg gcattaacca
    24901 cctttatcag tctggcgggt agctatctgg ttctgatgcc gaacaacccg cgcgcgggtg
    24961 gcatttctcg ccgtatcgaa ggcgacgacc gtaccgaatt aaaagaagca ctggcgagcc
    25021 ttgaactgcc ggaaggcatg gggcttatcg tgcgcaccgc tggcgtcggc aaatctgctg
    25081 aggcgctgca atgggattta agcttccgtc tgaaacactg ggaagccatc aaaaaagccg
    25141 ctgaaagccg cccggctccg ttcctgattc atcaggagag caacgtaatc gttcgcgcat
    25201 tccgcgatta cttacgtcag gacatcggcg aaatccttat cgataacccg aaagtgctcg
    25261 aactggcacg tcagcatatc gctgcattag gtcgcccgga tttcagcagc aaaatcaaac
    25321 tgtacaccgg cgagatcccg ctgttcagcc actaccagat cgagtcgcag atcgaatccg
    25381 ccttccagcg tgaagtccgt ctgccgtccg gtggttccat tgttatcgac agcaccgaag
    25441 cgttaaccgc catcgacatc aactccgcac gcgcgacccg cggcggcgat atcgaagaaa
    25501 ctgcgtttaa caccaacctc gaagctgccg atgagattgc tcgtcagctg cgcctgcgtg
    25561 acctcggcgg cctgattgtt atcgacttca tcgacatgac gcctgtacgc caccagcgtg
    25621 cggtagaaaa ccgtctgcgt gaagcggtgc gtcaggaccg tgcgcgtatt caaatcagcc
    25681 atatttctcg ctttggcctg ctggaaatgt cccgtcagcg cctgagccca tcactgggtg
    25741 aatccagtca tcacgtttgt ccgcgttgtt ctggtactgg caccgtgcgt gacaacgaat
    25801 cgctgtcgct ctctattctg cgtctgatcg aagaagaagc gctgaaagag aacacccagg
    25861 aagttcacgc cattgttcct gtgccaatcg cttcttacct gctgaatgaa aaacgttctg
    25921 cggtaaatgc cattgaaact cgtcaggacg gtgtgcgctg tgtaattgtg ccaaacgatc
    25981 agatggaaac cccgcactac cacgtgctgc gcgtgcgtaa aggggaagaa accccaacct
    26041 taagctacat gctgccgaag ctgcatgaag aagcgatggc gctgccgtct gaagaagagt
    26101 tcgctgaacg taagcgtccg gaacaacctg cgctggcaac ctttgccatg ccggatgtgc
    26161 cgccagcgcc aacgccagct gaacctgccg cgcctgttgt agccccagca ccgaaagctg
    26221 caccggcaac accagcaact cctgcacaac ctgggctgtt gagccgcttc ttcggcgcac
    26281 tgaaagcgct gttcagcggt agtgaagaaa ccaaaccgac cgagcaacca gcaccgaaag
    26341 cagaagcgaa accggaacgt caacaggatc gtcgcaagcc tcgtcagaac aaccgccgtg
    26401 accgtaatga gcgccgcgac acccgtagtg aacgtactga aggcagcgat aatcgcgaag
    26461 aaaaccgtcg taatcgtcgc caggcacagc agcagactgc cgagacgcgt gagagccgtc
    26521 agcaagctga ggtaacggaa aaagcacgta ccaccgacga gcagcaagcg ccgcgtcgtg
    26581 aacgtagccg ccgccgtaac gatgataaac gtcaggcgca acaagaagcg aaggcgctga
    26641 atgttgaaga gcaatctgtt caggaaaccg aacaggaaga acgtgtacgt ccggttcagc
    26701 cgcgtcgtaa acagcgtcag ctcaatcaga aagtgcgtta cgagcaaagc gtagccgaag
    26761 aagcggtagt cgcaccggtg gttgaagaaa ctgccgctgc cgaaccaatt gttcaggaag
    26821 ctccagctcc acgcacagaa ctggtgaaag tcccgctgcc agtcgtagcg caaactgcac
    26881 cagaacagca agaagagaac aatgctgata accgtgacaa cggtggcatg ccgcgtcgtt
    26941 ctcgccgctc gcctcgtcac ctgcgcgtga gtggtcagcg tcgtcgtcgc tatcgtgacg
    27001 agcgttatcc aacccagtcg ccaatgccgt tgaccgtagc gtgcgcgtct ccggaactgg
    27061 cctctggcaa agtctggatc cgctatccaa ttgtacgtcc gcaagatgta caagttgaag
    27121 agcagcgcga acaggaagaa gtacaagtgc agccgatggt gactgaggtc cctgtcgccg
    27181 ccgctgtcga acctgttgtt agcgcgccag ttgttgaaga aatggctgaa gtcgtagaag
    27241 cccccgtacc ggttgccgaa ccgcaaccgg aagtggttga aacgacgcat cctgaagtga
    27301 ttgccgccgc ggtaactgaa cagccgcagg tgattaccga gtctgatgtt gccgtagccc
    27361 aggaagtcgc agaacacgca gaaccggtgg ttgaaccgca ggaagagacg gcagatattg
    27421 aagaagttgc cgaaactgct gaggttgtgg ttgctgaacc tgaagttgtt gctcaacctg
    27481 ccgcgccagt cgtcgctgaa gtcgcagcag aagttgaaac ggtagccgcg gttgaaccag
    27541 agatcaccgt tgagcataac cacgctaccg cgccaatgac gcgcgctccg gcaccggaat
    27601 atgttccgga ggcaccgcgt cacagtgact ggcagcgccc tacttttgcc ttcgaaggta
    27661 aaggtgccgc aggtggtcat acggcaacgc atcatgcctc tgccgctcct gcgcgtccgc
    27721 aacctgttga gtaataatta gctcaaagta atcaagccct ggtaactgcc agggcttttt
    27781 tatttcatct ttgaatcaac atattaaaat tcatcaaatg gcatccttgc taaccaacaa
    27841 tgcaaaatag gcaaaaaaat gcccgccccg gcgatcccag agcaggcaga caaaacatac
    27901 ccagtttcat gatatgtttt aaagcgagct tatttgttga gctggaagag cgacaatccc
    27961 tgcatatcgg taaatgcttt atacgatgcc tgcaatgccg tttgctgcat gatgtaagat
    28021 gaaatagttg cattccagtc cacatcaacc agatcgctca tctgctgcgt ttgccctaaa
    28081 gcgcgatcgc tacctaatga atccagcgac tccagttcgt tcagctgcgt gcctaattcc
    28141 gcgcgaactg taaggacatt attcagcgag tttttcagtc cacggttggt tttatccaat
    28201 gccgcagcgg cgatttcttt atccgcttcg ctatccgcga ccggcgtttt cagggctgcg
    28261 atggcgctat ccagcatggc aaaaagattg gtttcagaag cgctaccgtc tggttccgct
    28321 accgcgttac tggtaatact gtcgaaaatt ttgtcgcccg tatgtcctat caccatcgaa
    28381 cgcgaagcat cgacctgttg cctaatactt tctgctccac cgacgtattt ccctttctct
    28441 tcgctaaacg gcgcagtctc tgttttataa ccggcaaaaa tgtagcgccc gttaccgtca
    28501 gtggtgttcg ccagattcag caactggtca cgaagcccct ggatatccgt agccagcgag
    28561 gcccggtcat cgtcactcaa agtgccattg ctggcgtaga caattttttc ctgagcattc
    28621 tggatagcag tggtgacctg gctaagtaca ctctcttcca gtgacacttt ttgagtggcg
    28681 aaagtacgcg ccagcgtgta ctggctgttt tgcgcctgtg cctgggagag aacaaccgct
    28741 tgtgatgctg caatgggatc gtcagaaggg ttaacgactc gcttacctgt cgacatctgc
    28801 tcgccgtact tcatccattc cgcctgagaa ttggtgatgc cacgcatgtt ttgctggtac
    28861 atcatctggg tactgaaacg catcttatct ccccttagcg aatgttaatc agcgcatcaa
    28921 aaatcgcgtt tgccgtctgc agaacctgcg cattcgccag gtaatactgc tgaaaacgtt
    28981 gcagatttcc gtactcctca tcgagattga caccggaaat cgactgctgc tgattggaaa
    29041 gctgcgtcac cacattacct tgcgtggcgc tactggtttt caacgtcgcg gttttattac
    29101 cgatatcact cactaacgag gcataagcgt cgttaaagga tttcgcaccg cccaccgttt
    29161 tactgttgct ttgcagatcc agcagggcct gaccgttgcg gttatcgcta tcacccgcat
    29221 cttcttcgct cgccatcgct attttcgctt cgtcggtgat taatacatcc atgttgacga
    29281 tagcgtcact tactggtttc agcgtgaagc tgtcgttaac agcaggcgtt cctgtaaacg
    29341 tcaactccag accatcaaat gccactttac cgttggcatc cggcgtcacc gtaaaagtgg
    29401 tattactggc aaggcgggtg acctgccact gattattatc gaacgagatt ttgtaatcag
    29461 tcgccagtac cgcggaggca tcagttaccg tggcaccgat cgcaacgtca cctgtgtttt
    29521 tcgtgttttg cagaaccgcg ggcttaccga tagcaaagaa atcttcacca gcatcgccgt
    29581 tggcatcaaa tccggctttg tgttggctgt tgaaagcctc ggcaaatgcc agcgccagtt
    29641 gtccaagcgt attacgcgtc tggtccagat cctgagaacg gaatgtcaga atgccgccca
    29701 gcgacccggt attcagtaat ttctccggga tctcaatatt gcctgccgtc ccatcaacgt
    29761 aagcgacagt cgtacgagaa gggtcggcgc tggaaggaac tgccgccagt tgccgcgccg
    29821 tacttccctg aaccagtgag taaccattgg ccatcgtgat gttataagta ccgccatcct
    29881 gaacgctgac ttctacgcca acaatctggt ttaattcgct caccagttga tcgcgttgat
    29941 ccagcagatt gttaggtgac gcccctgccc ccacgcctgt caggcgcgag atttgatcgt
    30001 tcaggctggc aatttgttta gcgtaattgt tgatctgatc aacgctggca cctatcgcga
    30061 tattgacctg tttgtcctgg tcgcgcagat attgatcggt ggttttaaac tgattcacca
    30121 acccttctga tttcccaatc agcgcctggc gcgctgccgg gtcttccgcg ttactcacca
    30181 gcgtttgcag gctggtgaag aaatcctgca tctgtgttgc cagcgaagag gtactggtgg
    30241 agagcatatt gtcgattttc gacatctgct catagcgggc agtcaggccg ctactttgcg
    30301 tctgcgccgc acgtaactgg ttggtaataa aagcatcata ctcacgctgc acaccagaaa
    30361 cgtagacacc attgccaacc cagccgccag cgcccaacgt gctattggcc tgcgccataa
    30421 tagtggtttg gcgggtatat ccggcaacgt tatagctgga gatattatta cttgccgtat
    30481 ttaacgccgc ctgggccgcg ttcagtccgc tcatggcgtt attgatcaag ctggacatgg
    30541 aggttcctta taagccttca attacagagt attatcggca gcgacccgcc ggacttgagt
    30601 tattcagaac agattatcaa tgttcatgct gtaggttttg ctcaccttgt cgcttatcga
    30661 tttcatctgc tggatcatgt tggtgagttt acgggcatag tgaggatcgg tagcataccc
    30721 cgcgtcctgt agggcctgcg ccccctgctc cgcactcgcg gcggtcgtca cggcggcgta
    30781 gcgcgggtta cgtgttaaca gcccaacgta atccgacaat gcttccagat acgagctgta
    30841 gacccgaaac ttcgctttta ctttcttcgc ttcgccgttt tcatattcag tcgtggtgat
    30901 ttcagtaact ggccctttcc agttgccaga ggctttgaca ccaaacaggt tatagctcgg
    30961 ctcgccgttt tcgcggcgga tttgccgttg cccccaacca gattccagtg ccgcctgagc
    31021 gaggatcaaa tgatgtggca caccgctttg ctggcttgcc agttgggcgg gcagcgagag
    31081 ttgcgcgagg aatgctttac tgtcacccgg cagcgaatca tcgtagttac gtggcacggc
    31141 cttttgcacc agctgcgaaa gcgcctgatt ttgataacgc accacagttt cgagcgggaa
    31201 tttcatcggt gctgctggcg tggactcctc tggcaatggt tgttctggcg tcatctgttt
    31261 aaccatcatc tctgcaagcc ccagaccttt gcccgccgtc atctgttggg caatctgctg
    31321 gtcatacata ctggtataca ggcgagtgtg ctcgctgctg aacaggccat cttttggtaa
    31381 agcgtcgcgc atgcttttca acatcatctg cacgaacatc ccttccacct gacgggccac
    31441 cggacggata tttgccgccg gatcttcgcc cgctttcgcc tttagttcgt tgagtgattg
    31501 cgcatcccag gccgcacttg ccagtagttt gctgtcgctg atcatcagat gatttccagt
    31561 tttgcccgca gacatcccgc actttgcatt gattgcagta tggacatcag atccatcggc
    31621 gtagcgccca gcgcattgag cgcgcgcacc acgttattga ggctggcgct ggaacgtacg
    31681 ctttgcagcg aaccgccgct ctggcgtaaa tcgatctgcg tttgtggagt aaccacagtc
    31741 tgtccaccac caaacggtgt atctggctgg ctgacattgg cctgacgatt gactgttact
    31801 gagagatttc cctgcgctac cgcgcagctg tcgagggtca cttcgcgatt catcaccacc
    31861 gaaccggtgc gcgagttaat cactacttta gcgtcctgcg gggtgacatt aacctgcata
    31921 ttctggatat ctgcaaggaa acggaccagg gaactgttgc cacttggtac gcgcacctga
    31981 atagtccgcg catctaacgc ggtggcgctg ccatatccac gcacgcggtt gatggtgtca
    32041 gcgatttgct gcgccatgct gaaatcttcg tcgttaagtt gcaaattaag ggtattcccg
    32101 acgccaaact ggctgggcaa ttcacgttca ataaccgcac cattggtgat ccgtccaccg
    32161 ttcagttggt tcacctgaac actgctaccg ccagcggagg ctcctgcgcc gccaaccaga
    32221 atattgccct gcgccagcgc atacacctga ctgtcaacgc ccttaagcgg cgtcatcaac
    32281 aacgtacctc cacgcaagct tttggcattt cccatggaag aaaccaccac atcgatggtt
    32341 tgcccctgac gtccaaacgg aggaagtgac gctgtcacca ttaccgcagc gacgtttttt
    32401 agctgcatat tggtgcccgt cggaacggta attcccagct gtgagagcat gttattaagc
    32461 gtttgtgtgg taaacggcgt ctgggttgtc tggtcaccgg tgccatccag ccccaccacc
    32521 agaccatagc caatcagtga gttttgcctt accccctgaa cactggtgag atcgcgaata
    32581 cgctcagcct gagccgccgt cgtaaccagt agaagaatta atgcagagag aaatttaatc
    32641 accacagcct cacttacatt ggcgacaggt taaggaagaa acgctgcaac cagcccatat
    32701 tttgcgcttc gttaatgtag ccattgccta cgtattcaat gcgcgcatcc gccacctgag
    32761 tagacggtac ggtattgctg ccgctgatag tgcgtggatt aaccacgcca gagaagcgaa
    32821 taaattcggt accctgatta atggcaatct gtttttcacc caccacatgc aggttgccgt
    32881 tgaccagtac ctggtcaacc gtcaccgtca acgtgccgct aaaggtattg ctggcattgg
    32941 ccccgccctt tccgttgaac gtgttaccac cggaggcttc gacatcggca cgagcgttac
    33001 caaacagccc ctgcaaatag cgcggcacag tatcaaagcc aaaattagtt ttaccgtcac
    33061 ggctggcatt cgcagaggag cttttgctgg cgctgacgtt ctcctgcaac acgatggtca
    33121 gcgtatcgcc aatattgcgt ggtcgacgat cttcaaacag cggttgatag ccatagttaa
    33181 tcggctgagc agactggaaa atagaaccgt tggcgacggg cgtcggcccg ggaaccggtt
    33241 gtgcactggt cgccccctgc accagcggcg tggagggtat ccaggcgcag ccggttagtg
    33301 aaagcaccaa caagctggaa atggcataag tatgcgcagc gtttttttgc atggctatca
    33361 tcttcaaaaa tcagtaaacc ggtgaacctg ccaccggtta agccttagag ttgcgtcagt
    33421 ttttgcagca tctgatcggt ggtggacacc gctttactgt tgatttcgta agcgcgttgc
    33481 acctgaatca tattgaccag ttcttccgcc acgttgacgt tagacgtttc aacataccct
    33541 tgatacagca gtcccgcgcc gttcaggccc ggcgtgcttt cgttcggtgc accagaggat
    33601 tgcgtttcgg tgtagaggtt ttcgccaatg ctctccagcc cggtgtcatt cataaaggtg
    33661 gtgagattga gctgcccaac ctgaaccgga gctgcctggc cttgttgggt tacgctgacc
    33721 acgccatcac gaccgatggt gatacttaac gcattcgccg gaatggtgat cgctggctgc
    33781 acctgaaaac caccagccgt caccagctgc ccgttctgat ccacctggaa agagccgtca
    33841 cgggtatagg ctgatgaacc atctggcaac atcacctgga aaaagccctg ccctttaatc
    33901 gcgacatctt tgctgttgtt ggtctgcgac aggtttccct ggctgtgtaa gcgttcagtg
    33961 gcgaccgggc gtacccccgt gccgatttgt aatccggagg gtaaggtggt ttgttcggaa
    34021 gactgtgccc ccggctggcg aatggtttga taaagcagat cttcaaacac cgcgcgctga
    34081 cgcttaaaac cgttagtact gacgtttgcc aggttgttgg caatgacgtc catattggtt
    34141 tgctgggcgt caaggcccgt tttggcgatc cataatgaac tgatcataaa atgtcctgta
    34201 gtggattagt gacaagcaga gcagaagcac gcgacagtgt gaatttcccc tcaccctaac
    34261 cctctcccca aaggggcgag ggaatagtcc gcgcgctttt attgcaatgt catcaatcgt
    34321 tcaattaact catcgacagc agttggttgg cacggcctgc gttatcatcg acgctgctga
    34381 tcaccttcat ctgcatttca aaacgccgcg cgctggcaat catgtcgctc attgccgcaa
    34441 cggcattgac gttactgcct tccagaaccc ccgacatcac acgcaaggtt ggatctgcct
    34501 gcagtatcgg cccacgcgtg gcctggcttt ctgcgcttaa acgaaaaatg ccgtcgtcac
    34561 cgcgttgcac ttcgctgccc gtggctttca ccagtttcag acgccccact ggcgcaaccg
    34621 tatttgccgg atcgcccgga ttgagcgccg agattgtgcc atcggcagca atagtgattt
    34681 ccgccccttc cggcacagca attggcccag cctcgcctat caccggatgc ccctgaattg
    34741 tcagttgccc ggtgggatca acctgaatgc tgccattacg cgtataccct tcgctgccgt
    34801 cagcggactg cacggccagc cagccatcct gctgcaatgc aacgtccagc gggcgcgagg
    34861 tgtaatccat cttgccgggc gtcatgtctg cacccggtgt tgacgccgtt accagcgtgc
    34921 gcgtgggcag tgaaagccct tccaccggca ctgcacgcaa ggcgtttaac tgcgcgcgaa
    34981 aaccgggcgt agaggcattg gccagattac tggcggttac cgcctgttga ttcagcgtct
    35041 ggctggctgc tcccatcgcg gtataaattg cgtgatccat tgagctatcc cgtcagcgat
    35101 tagcgtaagt taaccagcgt gttgaggatc tggtcctggg ttttgatggt ctgggcgtta
    35161 gactgatagt tacgctgggc aacgatcata ttgaccagtt ctttactgag atcgacgttg
    35221 gacgcttcca acgcaccgtt ggtcagggtg ccaaagttgc ccgtcccggc tgtccccaac
    35281 agcgccacgc cagaagattg cgtcgcagac cagacgttgt cgccttcgga tgccagacct
    35341 tcgttgttgg caaagttcgc cagtacaatc tgccccagca gttgtgtttg ttcgttggaa
    35401 tagttgccga caaccgtacc gtcatcattg atttgataac tcaccagatc gcccggtttg
    35461 tagccattct gggtggttgc cacaatattg ttagcgccgg tattttgctg catggagttg
    35521 aggaagctca ggctaaacgt ggcgggatct gcgccgttaa ttgcgccagt ggtaatattc
    35581 tctgttggat ttgaggtcag aacgccattg gcattaaaca ccagcttcat tgcaggctcg
    35641 gctgtacctg ttggatcact gctatcctgg gtgtagacct gccagttatt atccccggtc
    35701 ttcacaaagt agacgctcat gtcatgagca ttaccctgac tgtcgaaaac agtcaccgaa
    35761 ccttttttgt tatagctatc cgcattgctg gcgctgaatg gcgtaacagt aggaagcgga
    35821 tcactggaat tcaggttgat ctgcatcgat gccgtggtgg tagttttcgc tgccatcagg
    35881 gtattcggga tcgaaatatt ggtcggattc gccccttgct gaatagtcgg cggcgtaccg
    35941 gttgccgggt aacccgtcag ctgtaaacct tgcatattca ccaggttacg gttttcatcc
    36001 agcttaaatt gtccgttacg gctgtagaac accgaaccgt tgctgtctac cagacggaaa
    36061 aaaccgttct ggctgatagc aacgtccaga cctcgcccgg tgttggtggt cgtgccatcg
    36121 gtaaagtcct gagtgatacc ggcaactttt acccccagtc ccactttcga accggcaaac
    36181 atatcggcaa aagaggccgt gcctgattta aagccgtagg tggcggagtt ggcgatattg
    36241 ttgccaataa catcgaggtt ggtggcggca gcgtttaatc cgctaaccgc ttgagaaaag
    36301 gccatgactg actcctgaaa tgtgaaggct taaattatct gccgtacttc gtcgagggtg
    36361 gtggtgccgt aagtgccgag atccagcgta ttaccgctgt tgccgcggat cacaccctgc
    36421 accagagcaa actgcagcgg ctgggcaacc agttgtgtac caccgttact ggcgctaatc
    36481 gctacattgt aagaaccgtt cggcgcagtg ctgccgtcag tcaacgtacc gtcccaggtg
    36541 aaactgtgaa ctccggcggt cagttcacca atatcaatgg tgcgcacaac cgcaccattt
    36601 ttatcggtga tggtggccgt aactttgtct gccgcctgtt gcagctcaac accaaacggc
    36661 gtggtcgtgg tcacagctcc ttcttcactg ccggttcccg caagaacagt ggtgccgggg
    36721 atcatcacgc cgtgaccgat caggttactg gcctgtaacg actggctgtt atcaatctgt
    36781 ccggaaatag atccgagtgt ggtattgagt ttttcaatcc cgctgaccgt gctgatttgt
    36841 gccaattgcg acgtcagctc gttgttttcc attggattgg tcgggtcctg gtttttcagc
    36901 tgcgccacca gcaaagtcag aaaactgctt tgtaaatctg cggcgttgct gcccgtgagc
    36961 gaactactgc tggtggtact gacgccggta tttgtcggat cggtggtggt taccgcaatg
    37021 gacatagctt tctcctttat tgaccgagcg taagggtttt cagcatcatg cttttcaccg
    37081 tgttgagcac ttcaacgttg gcctgatagc tgcgtgacgc cgacatggtg ttaaccatct
    37141 ccccgacgac atcaacgttc ggcattttta cgtagccctt tgcatctgcc agcggattac
    37201 ccggttcata aaccagtttg tccggggcct gactttctat aacatcggca acctttacgc
    37261 cgcctgtcgc agcacctggt gcagcgttaa cctggaatac cacctgtttt gcccgatatg
    37321 gctgtccatc aggaccggtc acgctatcag catttgccag attactggcc gccacgttca
    37381 ggcgctggga ctgggcagtt aacgccgacc cggcgatatc aaaaatattc agcagtgcca
    37441 tccgttaatt tccgctctgt aaaacgttca tcatgccttt gatttgcccg ctcaacgcgc
    37501 taaggctcat ctggtattgc aggctgttat cggcaaactg ggtgcgttcg cgatccatat
    37561 cgacggtatt accgtcaagc gaaggctggt ccggaatacg gtattgcagt tctgcggtag
    37621 gaggcgtcag cgcctgcgcc gggatgtgtt gcgttgaggt catcgtcagt gcaaccacac
    37681 tggttgcatc ccgtccacgt tgcatgactt ttttaagttc actggcaaaa tcgatatcgc
    37741 gcgcctgata accaggggta tcggcattgg cgatgtttgc tgccagcact tcctgacgct
    37801 gggcgcgcag attgagcgcc tcttgttgaa aacgtaaggc ggcgtcgagc ttatcgagca
    37861 tatctcctcc gcaggtatca aaattctgcc atcagcttaa atgcctttac gcgcgcgtta
    37921 tcggcggaat aaacgcaaaa tgggtcgcta tttatgccgt tgatggtcat tgcggacagg
    37981 tacaattcac gttgtagaaa tggctggggg cgaaaatgct gacaataaaa cgtagcgtgg
    38041 cgatcatcgc gatactgttc agtccgttaa gtacggcgag caatctcaca tcgcaattgc
    38101 acaacttttt tagcgcccaa ctcgcggggg taagtgatga ggttcgtgtt tctattcgta
    38161 cagcgcccaa tctactaccg ccatgcgagc agccattgct ttcgatgagc aataattccc
    38221 gcctgtgggg caatgtgaat gtgttggcac gctgcggtaa cgacaaacga tatttacagg
    38281 ttaatgtgca ggccacagga aattatgtgg ttgccgcgat gcccattgtg cggggaggaa
    38341 agctggaagc tggcaatgtc aaactaaaac gcggacggct ggataccctg ccaccgcgta
    38401 cggtgctgga tatcaatcaa cttgttgatg ccattagcct gcgcgatcta tcacccgatc
    38461 aacctatcca gttaacccag tttcgccagg catggcgggt aaaagcggga caacgcgtca
    38521 atgtgatcgc cagcggtgat gggtttagcg ccaacgcaga aggtcaggcg ctgaacaatg
    38581 cagccgtcgc acagaatgcg cgggtgcgca tggtatcggg acaggtagtc agcggcgttg
    38641 ttgatgcaga tgggaatatt cttataaacc tgtaagctgt taaagattac ccgtcccttg
    38701 ccgataaata agcaacacat gataaaagcg ccctcaatga ggaataaacc atgagtattg
    38761 atcgcacttc gcctctgaag cctgtaagca ccgttcaacc gcgcgaaacc actgacgcgc
    38821 cggtaacgaa cagccgggcg gcaaaaacaa ccgcctccac cagcaccagt gtgacgttaa
    38881 gcgacgcgca agcaaaactg atgcaacccg gcagcagtga tatcaatctt gaacgtgtcg
    38941 aagcgttaaa actggcgatt cgtaacggtg aactaaaaat ggacaccggc aaaattgccg
    39001 atgcgctgat caacgaagcg cagcaagact tgcagagtaa ctgatcgtat gacacgtctt
    39061 gcagagatcc tcgaccagat gtccgctgtg cttaacgatc tcaaaacggt aatggatcaa
    39121 gagcagcaac atctctctat ggggcagatc aacggcagcc agttgcaatg gattacagaa
    39181 caaaaaagct cactgctggc gacgctggat tacctcgaac agttacgcaa gaaagaaccc
    39241 aatacagcaa atagcgttga tattagtcaa cgctggcagg aaattactgt gaaaacgcag
    39301 caactacgcc aaatgaatca acataacggc tggttactgg aaggacagat tgagcgcaat
    39361 caacaggcgc tggaaatgtt gaaaccgcat caggaaccga cgctatatgg ggcgaacggt
    39421 cagacctcaa caacccatcg cggcggtaaa aagatttcga tctgaatatt atctccggcc
    39481 tgcactgcag gccggaatgc attgttacac cgtccggcgg gcaaattctt taactttgaa
    39541 gcccagtacc gccagtgcag cgaagtacgc ggcaatcccc gccagcacga ccgccattaa
    39601 acgcagtaag cgccagggca tggtacccaa tgaccactcc ggcatgatat gtaacatacc
    39661 taaaagcacg ccagacatca ccagtaccgc caccaccaga cgcaacagaa acgccatcca
    39721 gccgggttgc ggagtaaaga ttttctgctt acgcaactgc cagtaaagca gcgaagcatt
    39781 cagacacgcc gccagaccaa tagaaagtga cagcccggca tgtttcaacg gaccaataaa
    39841 cgccaggttc atcaattgcg ttaaaatcag cgtaacgatg gcaattttca ctggcgtttt
    39901 aatgtcctgg cgggaataaa agccaggagc caacactttc actacaatca ggccaatcaa
    39961 acccaccgag taggcaatta acgcccgctg ggtcatcagc gcatcaaacg cggtaaattt
    40021 accgtactgg aacagcgaaa cggttaacgg accagaaaga atgcccaacg caaccgcact
    40081 cggcagcgcc aacaggaaac aaagacgcaa cccccagtcc atcaaacggt tgtattcatc
    40141 atgattgcca ctggcaaaac ttttcgacag cgacggcagc aaaatggtgc caagcgccac
    40201 gcccagcaca ccggacggaa actccattaa gcggtcggcg taatacatcc aagacaccga
    40261 accggaagca agaaacgagg caaaaatggt gttgatgatt aaggagatct ggctcacaga
    40321 gacgccaagg atcgccggtc ccatctgttt caccacgcgc attgttccgg catcgtggaa
    40381 gttaatgcgc ggcaggacca gcatgccgat cttcttcagg tgcggtagct gatacaccag
    40441 ctgcaggatg ccgccgaccg taacagccca cgccagcgcc agcaccggtg ggttaaagta
    40501 cggtgcggca aacagcgcga aaccaatcat gctgatgtta agcagtgttg gagcaaacgc
    40561 cggaatcgag aagcggttcc acgtattcag aatcgctccc accagcgacg ccagggagat
    40621 cagcaagata tagggaaagg taatctttag tagctggctg gtcagggcaa atttgtcagc
    40681 tgtgtcagcg aagcctggcg cggtcaccat gatcacccac ggtgcggcga gcatgccagc
    40741 gaccgtcaca accgccagcg cgagtgtcag caggccagaa acataagaga caaagacccg
    40801 cgtggcgtct tcaccctgct tacttttata ttccgccaga atcggtacaa atgcctggga
    40861 aaatgcccct tcggcaaaga tacggcgtaa caagttagga agtttaaaag cgacgaaaaa
    40921 ggcgtcggtt gccatccctg cgccaaagat tctggcgaca attgcgtctc gtgcgaagcc
    40981 aagcacacgc gaaaacatgg tcatcgagct gacggcggcc agcgatttta ataaattcat
    41041 cggtgttcta atccagaccc acgggcaaaa cgcctgcaag gcaggcgctg aaagaggcga
    41101 atagtctacc tggattatgg tgaattgcta ccgccagatg ttacagggtt tattcactca
    41161 tcgcatcgcg ccagatcttg tcaacgatac gttgcgccag cacggcctgt tcgccggcgg
    41221 tttgcggaac tgtctgattt tgcacacatt caatgaagtg ccgcgcacag ccgacaaatc
    41281 cacgttgttc aagagtgctc tgccaaccag gaatcggttt atgcactacg ccctgcccgc
    41341 gctcctcacg ccattcgcgc atatccgtaa tgtcgatgag cgcaccgtca gtcacggcct
    41401 gcacggtttc acgctgactt ccggcacggc gatgcataca ggtggtgatc tgcaaaggac
    41461 cggccgaaaa atggtgctcg gcaaacagca tttcgccagc gtcgttagtc agtagcgtac
    41521 cgccatccag agaggctttg ccgcccgaca accacagcac ggtatccacc acatgcagat
    41581 aatcatccag caacgtgaaa taaagatcgt gtggcccgac actattgcta cgatgtttat
    41641 ccattcttag cgaagctgcg gtagcgagtt gcgtttttaa ctcaccgtag agtggtgcga
    41701 aacgacggtt aaaaccgacc atcaacgtca gttttttacg cgccgccagt tccaccagcc
    41761 gttcagcatc gcgcagattt tctgccagcg gtttatcgac acagacatgt acacccgcat
    41821 tgagtaacgt actgaccacg tcaaagtggc tggcggtgct ggaatgcaca aaaaccgcat
    41881 cgcaactggc ggcaaggctg gataacgaat cggcataagg aatgcgccag ctttcacaaa
    41941 ttggcagggc tttcgcgcgc gtaggcgacc aggctccttg taacgtccag tcagacgctg
    42001 ccgccagcac cggtaaccac gctttttgcg caatgccacc taatcccact acgccgatac
    42061 gtaatttttt cactttaatc tcccagatgg gccagcaacg aatcaagacg ctgtttcagt
    42121 tctgccactt cgatttccag ggcttcgacg cgggcctgta aatcaccatc aaccgcgttc
    42181 gacatatccg tcaccgccgg ctgatcttca acctcaccac tgaaaagatg catgtagcgg
    42241 ctttcgcgtt tacccggttc gcgggccaga cgcaccacaa aaggaccatc ttcgcgattt
    42301 gccagttgtt ccagcgtcga ctccacttcc gccatatcgc tgaattcata cattcgcgcg
    42361 gcgcggctgc gcagttcacc tggcgtctgg gcaccacgca ataacaacgt ggtgattaac
    42421 gccacttccg ctgcgctcag tttcagatcg ccaaattctg aattacaaaa acgttgctca
    42481 tatttggtga cccgattacc aaaaccgctc actgtgcgta gataatgacg tttgaccaga
    42541 ttatccagct gttcctgcac ttcggattca ctcagattca tgaccggttc acggttcgtt
    42601 ttctgattac aggccgtgac tacaccattg actgagagcg ggtattgctc cggcgtggtc
    42661 acctgctttt ccagcaagca accaatcacg cgagcttcaa gggcagtaag ttgatatttc
    42721 atgagattct ccttagcggc cgggcgtcca gtctggggta gttaatgccg tcagtacgtg
    42781 atcgcgccat tgtccatcaa tcaacagata gtctttcgca tagccttctt tttcaaaacc
    42841 cagtcgcgcc agtaaatcac cgctgcgttt attgtgcggc atataattag ccataatgcg
    42901 atgaatatgt tgggtgcgct gcatataacg aatggctgcg gtcagggctt caaacatgag
    42961 tcctttgccc tgccattttt gcccaatcga ataaccgaga tagcaggcat gaaaagagcc
    43021 acgaacaaca ttggaaaaat tggcaacgcc aataatctct ttttcgtccg ggtcgaataa
    43081 gccaaagtag aaagctgaac cttgtttatg aaattcgtta atcatcccca gcctggcctg
    43141 ccagcctgat ggataacagt ggctttcgtc gcgcactggc tcccagggct tgaggaaatg
    43201 gcgattctct gcgtaataat ccgcaagacg ccaggcatca cgatcatgca ccagacgcac
    43261 gaccagtcgg tctgtggtta agcgcacttt tggcacgtta ctgcgatagc caaacattct
    43321 atacctactc cttcccgtaa cgtctttaat acgctgtaag tattactata cctgcgcaac
    43381 cagaccctgt gaaaagtcca aaatgccatt ttctgcatta ttcacatatt tgataaaaac
    43441 tctctatcaa agcgacgttt tgataaaaaa atattgtccc cgaagtgatg ggaaaagtct
    43501 tagcggcaat gcagaataga aagtgctcat cttttatttc ccgggagggg aaatgtcccg
    43561 cgtatcgcag gcgaggaacc tgggtaaata tttcctgctc atcgataata tgctggtcgt
    43621 gctggggttc tttgttgtct tcccgctgat ctctatccgc ttcgttgatc aaatgggctg
    43681 ggccgccgtc atggtcggta ttgctctcgg tctacgccag tttattcagc aaggtctggg
    43741 tattttcggc ggcgcaattg ccgaccgctt tggtgccaaa ccgatgattg ttaccggtat
    43801 gctgatgcgc gccgccggat tcgccacaat gggtatcgcc cacgaaccgt ggctattgtg
    43861 gttttcatgc ctgctctcgg gactcggcgg cacgttgttt gatccgccgc gttcggcgct
    43921 ggtggtgaaa ctaatccgtc cacaacaacg tggtcgtttt ttctcgctgt tgatgatgca
    43981 ggacagtgcc ggtgcggtca ttggcgcatt gttggggagc tggctgttgc aatacgactt
    44041 tcgcctggtc tgcgccacag gggccgtttt gtttgtgctg tgtgcggcgt tcaatgcgtg
    44101 gttgttaccg gcatggaaac tctccaccgt acgcacgccc gttcgcgaag gcatgacccg
    44161 cgtgatgcgt gacaagcgtt ttgtcaccta tgtcctgacg ctggcgggtt actacatgct
    44221 ggctgtacaa gtgatgctga tgctgccaat tatggtcaac gacgtggctg gcgcaccctc
    44281 tgccgttaaa tggatgtatg ccattgaagc gtgtctgtcg ttaacgttgc tctaccctat
    44341 cgcccgctgg agtgaaaagc attttcgtct ggaacaccgg ttgatggctg ggctgttgat
    44401 aatgtcatta agcatgatgc cggtgggcat ggtcagcggc ctgcaacaac ttttcaccct
    44461 gatttgtctg ttttatatcg ggtcgatcat tgccgagcct gcgcgtgaaa ccttaagtgc
    44521 ttcgctggcg gacgcaagag ctcgcggcag ctatatgggg tttagccgtc tgggtctggc
    44581 gattggcggc gctattggtt atatcggtgg tggctggctg tttgacctgg gcaaatcggc
    44641 gcaccagcca gagcttccgt ggatgatgct gggcattatt ggcatcttca ctttccttgc
    44701 gctgggttgg cagtttagcc agaaacgcgc cgcgcgtcgt ttgcttgaac gcgacgcctg
    44761 atttgatact cccccctgac tcctccatac tgaaagtgaa ctgacagcca aatggaggag
    44821 tcacgtgaag ctatacattt acgatcactg cccttactgc ctcaaagccc gcatgatttt
    44881 cggcctgaaa aatatccccg tcgaattaca tgttctgctc aacgacgacg cagaaacacc
    44941 cacccggatg gtcggtcaaa aacaggttcc cattctgcaa aaagatgaca gccgctatat
    45001 gccagaaagc atggacatcg ttcactatgt cgataaactc gacggcaaac cgttactgac
    45061 cggcaaacgt tcccctgcca ttgaagagtg gctgcgcaag gtcaatggct acgcaaacaa
    45121 actgctgttg ccgcgttttg ccaaatcggc atttgatgag ttttctactc ccgccgcgcg
    45181 caaatatttc gtcgacaaga aagaggccag cgcgggtaat ttagccgacc tgctggccca
    45241 ctctgacggt ctgattaaga atatcagcga tgatttgcgt gcgctggaca aactgatcgt
    45301 caaaccgaac gccgtgaatg gcgaactttc ggaagatgat attcagctat ttccgctact
    45361 gcgtaatctg acgctggtag ccggaatcaa ctggccaagc cgcgttgctg attaccgcga
    45421 taatatggcg aaacagacac aaatcaattt gttatcatca atggcgattt aatcctgacc
    45481 cgccgcggca gcctgtattc gcgggttgcc gccgttttca gttttttctg ctggcgagtt
    45541 tatcgctggt gacacatgct gcgttgtgtc aggatattga cgcagatttc atgtcgagga
    45601 ctaccatgaa caagttttta tttgccgctg cattgatcgt cagtggcctg ctcgttggct
    45661 gtaatcaact cacccaatac accatcaccg aacaagaaat taaccagtcg cttgcgaaac
    45721 ataataattt ctcaaaagat atcggtttac ccggcgtggc tgacgcccat attgttctga
    45781 caaacttgac cagccaaatt ggtcgcgaag agccgaataa ggttacccta accggagacg
    45841 ccaatctgga catgaactcc ctgttcggta gtcagaaagc gaccatgaaa ctgaagctga
    45901 aagcgctgcc cgtgtttgat aaagaaaaag gtgcgatctt cctgaaagag atggaagtgg
    45961 tcgatgcgac ggtacaaccg gaaaaaatgc aaacggtgat gcaaacgttg cttccctatt
    46021 tgaaccaggc attacgcaat tactttaacc agcaacctgc ttacgtcctg cgcgaagatg
    46081 gcagtcaggg cgaagcaatg gcgaaaaaac tggcgaaagg cattgaagtg aagccaggcg
    46141 aaattgtcat tccgtttacg gattaatgac gagggcgcat tcgcgccctt tatttttcgt
    46201 gcaaaggaaa acgtttccgc ttatcctttg tgtccggcaa aaacatccct tcagccggag
    46261 catagagatt aatgactgca ccatcccagg tattaaagat ccgccgccca gacgactggc
    46321 accttcacct ccgcgatggc gacatgttaa aaactgtcgt gccgtatacc agcgaaattt
    46381 atggacgggc tattgtaatg cccaatctgg ctccgcccgt gaccactgtt gaggctgccg
    46441 tggcgtatcg ccagcgtatt cttgatgccg tacctgccgg gcacaatttc accccattga
    46501 tgacctgtta tttaacagat tcgctggatc ctaatgagct ggagcgcgga tttaacgaag
    46561 gcgtgttcac cgctgcaaaa ctttatccgg caaacgcaac cactaactcc agccacggcg
    46621 tgacgtcaat tgacgcaatc atgccggtac ttgagcgcat ggaaaaaatc ggtatgccgc
    46681 tactggtgca tggtgaagtg acacatgcag atatcgacat ttttgatcgt gaagcgcgct
    46741 ttatagaaag cgtgatggaa cctctgcgcc agcgcctgac tgcgctgaaa gtcgtttttg
    46801 agcacatcac caccaaagat gctgccgact atgtccgtga cggaaatgaa cggctggctg
    46861 ccaccatcac tccgcagcat ctgatgttta accgcaacca tatgctggtt ggtggcgtgc
    46921 gtccgcacct gtattgttta cccatcctca aacgcaatat tcaccaacag gcattgcgtg
    46981 aactggtcgc cagcggtttt aatcgagtat tcctcggtac ggattctgcg ccacatgcac
    47041 gtcatcgcaa agagagcagt tgcggctgcg cgggctgctt caacgcccca accgcgctgg
    47101 gcagttacgc taccgtcttt gaagagatga atgctctgca gcactttgaa gcattctgtt
    47161 ctgtaaacgg cccgcagttc tatggcttgc cggtcaacga cacattcatc gaactggtac
    47221 gtgaagagca acaggttgct gaaagcatcg cactgactga tgacaccctg gtgccattcc
    47281 tcgccgggga aacggtacgc tggtccgtta aacaataaaa atcgtagctt cctgttgtca
    47341 ttaggttatt ttacctgtat aaataaccag tatattcaac agggggctat tatgcgaatt
    47401 gaagtcacca tagcgaaaac ttctccattg ccagctgggg ctattgacgc cctggctggc
    47461 gaactttccc gccgtattca gtatgcgttt cctgataatg aaggccacgt atcggtacgt
    47521 tatgccgcag cgaataattt atcggttatt ggcgcaacaa aagaagataa acagcgcatt
    47581 agcgaaattc tccaggaaac gtgggaaagc gccgatgact ggtttgtcag cgaataatat
//
LOCUS       DAZKHF020000008        47080 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2201S31241
            SAMN43267800-rid25106413.denovo.008, whole genome shotgun sequence.
ACCESSION   DAZKHF020000008 DAZKHF020000000
VERSION     DAZKHF020000008.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267800
            Sequence Read Archive: SRR30307263
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 47080)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 47080)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 47080)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350581.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350581.2
            Genome Coverage       :: 151x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:21:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,652
            CDSs (total)                      :: 5,557
            Genes (coding)                    :: 5,320
            CDSs (with protein)               :: 5,320
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 237
            CDSs (without protein)            :: 237
            Pseudo Genes (ambiguous residues) :: 0 of 237
            Pseudo Genes (frameshifted)       :: 102 of 237
            Pseudo Genes (incomplete)         :: 130 of 237
            Pseudo Genes (internal stop)      :: 52 of 237
            Pseudo Genes (multiple problems)  :: 41 of 237
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..47080
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267800-rid25106413.denovo.008"
                     /serotype="O 26:H11"
                     /isolate="2201S31241"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-01"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            103..960
                     /gene="focA"
                     /locus_tag="ACHOSC_RS01740"
                     /old_locus_tag="pgaptmp_000348"
     CDS             103..960
                     /gene="focA"
                     /locus_tag="ACHOSC_RS01740"
                     /old_locus_tag="pgaptmp_000348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate transporter FocA"
                     /protein_id="HGU4546362.1"
                     /translation="MKADNPFDLLLPAAMAKVAEEAGVYKATKHPLKTFYLAITAGVF
                     ISIAFVFYITATTGTGTMPFGMAKLVGGICFSLGLILCVVCGADLFTSTVLIVVAKAS
                     GRITWGQLAKNWLNVYFGNLVGALLFVLLMWLSGEYMTANGQWGLNVLQTADHKVHHT
                     FIEAVCLGILANLMVCLAVWMSYSGRSLMDKAFIMVLPVAMFVASGFEHSIANMFMIP
                     MGIVIRDFASPEFWTAVGSAPENFSHLTVMNFITDNLIPVTIGNIIGGGLLVGLTYWV
                     IYLRENDHH"
     gene            1015..3297
                     /gene="pflB"
                     /locus_tag="ACHOSC_RS01745"
                     /old_locus_tag="pgaptmp_000349"
     CDS             1015..3297
                     /gene="pflB"
                     /locus_tag="ACHOSC_RS01745"
                     /old_locus_tag="pgaptmp_000349"
                     /EC_number="2.3.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008861 - formate C-acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate C-acetyltransferase"
                     /protein_id="HGU4546363.1"
                     /translation="MSELNEKLATAWEGFTKGDWQNEVNVRDFIQKNYTPYEGDESFL
                     AGATEATTTLWDKVMEGVKLENRTHAPVDFDTAVASTITSHDAGYINKQLEKIVGLQT
                     EAPLKRALIPFGGIKMIEGSCKAYNRELDPMIKKIFTEYRKTHNQGVFDVYTPDILRC
                     RKSGVLTGLPDAYGRGRIIGDYRRVALYGIDYLMKDKLAQFTSLQADLENGVNLEQTI
                     RLREEIAEQHRALGQMKEMAAKYGYDISGPATNAQEAIQWTYFGYLAAVKSQNGAAMS
                     FGRTSTFLDVYIERDLKAGKITEQEAQEMVDHLVMKLRMVRFLRTPEYDELFSGDPIW
                     ATESIGGMGLDGRTLVTKNSFRFLNTLYTMGPSPEPNMTILWSEKLPLNFKKFAAKVS
                     IDTSSLQYENDDLMRPDFNNDDYAIACCVSPMIVGKQMQFFGARANLAKTMLYAINGG
                     VDEKLKMQVGPKSEPIKGDVLNYDEVMERMDHFMDWLAKQYITALNIIHYMHDKYSYE
                     ASLMALHDRDVIRTMACGIAGLSVAADSLSAIKYAKVKPIRDEDGLAIDFEIEGEYPQ
                     FGNNDPRVDDLAVDLVERFMKKIQKLHTYRDAIPTQSVLTITSNVVYGKKTGNTPDGR
                     RAGAPFGPGANPMHGRDQKGAVASLTSVAKLPFAYAKDGISYTFSIVPNALGKDDEVR
                     KTNLAGLMDGYFHHEASIEGGQHLNVNVMNREMLLDAMENPEKYPQLTIRVSGYAVRF
                     NSLTKEQQQDVITRTFTQSM"
     gene            3489..4229
                     /gene="pflA"
                     /locus_tag="ACHOSC_RS01750"
                     /old_locus_tag="pgaptmp_000350"
     CDS             3489..4229
                     /gene="pflA"
                     /locus_tag="ACHOSC_RS01750"
                     /old_locus_tag="pgaptmp_000350"
                     /EC_number="1.97.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043365 -
                     [formate-C-acetyltransferase]-activating enzyme activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate formate lyase 1-activating protein"
                     /protein_id="HGU4546364.1"
                     /translation="MSVIGRIHSFESCGTVDGPGIRFITFFQGCLMRCLYCHNRDTWD
                     THGGKEVTVEDLMKEVVTYRHFMNASGGGVTASGGEAILQAEFVRDWFRACKKEGIHT
                     CLDTNGFVRRYDPVIDELLEVTDLVMLDLKQMNDEIHQNLVGVSNHRTLEFAKYLANK
                     NVKVWIRYVVVPGWSDDDDSAHRLGEFTRDMGNVEKIELLPYHELGKHKWVAMGEEYK
                     LDGVKPPKKETMERVKGILEQYGHKVMF"
     gene            complement(4311..4901)
                     /gene="ycaK"
                     /locus_tag="ACHOSC_RS01755"
                     /old_locus_tag="pgaptmp_000351"
     CDS             complement(4311..4901)
                     /gene="ycaK"
                     /locus_tag="ACHOSC_RS01755"
                     /old_locus_tag="pgaptmp_000351"
                     /EC_number="1.6.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H oxidoreductase"
                     /protein_id="HGU4546365.1"
                     /translation="MQSERIYLVWAHPRHDSLTANIADAIHQRAMERKIQVTELDLYR
                     RNFNPVMTPEDEPDWKNMDKRYSPEVHQLYSELLEHDTLVVVFPLWWYSFPAMLKGYI
                     DRVWNNGLAYGDGHKLPFNKVRWVALVGGDKESFVQMGWEKNISDYLKNMCSYLGIED
                     ADVTFLCNTVVFDGQELHASYYQSLLSQVRDMVDAL"
     gene            5001..5909
                     /gene="ycaN"
                     /locus_tag="ACHOSC_RS01760"
                     /old_locus_tag="pgaptmp_000352"
     CDS             5001..5909
                     /gene="ycaN"
                     /locus_tag="ACHOSC_RS01760"
                     /old_locus_tag="pgaptmp_000352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0001216 - DNA-binding transcription
                     activator activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HGU4546366.1"
                     /translation="MRMNMSDFATFFAVARNQSFRAAGDELGLSSSAISHSIKTLEQR
                     LKIRLFNRTTRSVSLTEAGSNLYERLRPAFDEIQIMLDEMNDFRLTPTGTLKINAARV
                     AARIFLMPLLVGFTREYPDIKVELTTDDSLVDIVQQGFDAGVRLSGIVEKDMISVAIG
                     PPVKLCVAATPEYFARYGKPRHPHDLLNHQCVVFRYPSGKPFHWQFAKELEIAVAGNI
                     ILDDVDAELEAVLMGAGIGYLLYEQIKEYLDTGRLECVLGDWSTERPGFQIYYPNRQY
                     MSCGLRAFLDYVKTGQICQSQRHRPQ"
     gene            complement(5910..7340)
                     /gene="ycaM"
                     /locus_tag="ACHOSC_RS01765"
                     /old_locus_tag="pgaptmp_000353"
     CDS             complement(5910..7340)
                     /gene="ycaM"
                     /locus_tag="ACHOSC_RS01765"
                     /old_locus_tag="pgaptmp_000353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415419.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="APC family permease"
                     /protein_id="HGU4546367.1"
                     /translation="MAGNVQEKQLRWYNIALMSFITVWGFGNVVNNYANQGLVVVFSW
                     VFIFALYFTPYALIVGQLGSTFKDGKGGVSTWIKHTMGPGLAYLAAWTYWVVHIPFLA
                     QKPQAILIALGWAMKGDGSLIKEYSVVALQGLTLVLFIFFMWVASRGMKSLKIVGSVA
                     GIAMFVMSLLYVAMAVTASAITEVHIATTNITWETFIPHIDFTYITTISMLVFAVGGA
                     EKISPYVNQTRNPGKEFPKGMLCLAVMVAVCAILGSLAMGMMFDSRNIPDDLMTNGQY
                     YAFQKLGEYYNMGNTLMVIYAIANTLGQVAALVFSIDAPLKVLLGDADSKYIPASLCR
                     TNASGTPVNGYFLTLVLVAILIMLPTLGIGDMNNLYKWLLNLNSVVMPLRYLWVFVAF
                     IAVVRLAQKYKPEYVFIRNKPLAMTVGIWCFAFTAFACLTGIFPKMEAFTAEWTFQLA
                     LNVATPFVLVGLGLIFPLLARKANSK"
     gene            complement(7549..8697)
                     /gene="ycaD"
                     /locus_tag="ACHOSC_RS01770"
                     /old_locus_tag="pgaptmp_000354"
     CDS             complement(7549..8697)
                     /gene="ycaD"
                     /locus_tag="ACHOSC_RS01770"
                     /old_locus_tag="pgaptmp_000354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HGU4546368.1"
                     /translation="MSTYTRPVMLLLSGLLLLTLAIAVLNTLVPLWLAQEHMSTWQVG
                     VVSSSYFTGNLVGTLLTGYVIKRIGFNRSYYLASFIFAAGCAGLGLMIGFWSWLAWRF
                     VAGVGCAMIWVVVESALMCSGTSRNRGRLLAAYMMVYYVGTFLGQLLVSKVSTELMSV
                     LPWVTGLTLAGILPLLFTRVLNQQAENHDSTSITSMLKLRQARLGVNGCIISGIVLGS
                     LYGLMPLYLNHKGVSNASIGFWMAVLVSAGILGQWPIGRLADKFGRLLVLRVQVFVVI
                     LGSIAMLSQAAMAPALFILGAAGFTLYPVAMAWACEKVEHHQLVAMNQALLLSYTVGS
                     LLGPSFTAMLMQNFSDNLLFIMIASVSFIYLLMLLRNAGHTPKPVAHV"
     gene            9012..9638
                     /gene="ycaC"
                     /locus_tag="ACHOSC_RS01775"
                     /old_locus_tag="pgaptmp_000355"
     CDS             9012..9638
                     /gene="ycaC"
                     /locus_tag="ACHOSC_RS01775"
                     /old_locus_tag="pgaptmp_000355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isochorismate family cysteine hydrolase YcaC"
                     /protein_id="HGU4546369.1"
                     /translation="MTKPYVRLDKNDAAVLLVDHQAGLLSLVRDIEPDKFKNNVLALG
                     DLAKYFNLPTILTTSFETGPNGPLVPELKAQFPDAPYIARPGNINAWDNEDFVKAVKA
                     TGKKQLIIAGVVTEVCVAFPALSAIEEGFDVFVVTDASGTFNEITRHSAWDRMSQAGA
                     QLMTWFGVACELHRDWRNDIEGLATLFSNHIPDYRNLMTSYDTLTKQK"
     gene            complement(9674..10537)
                     /gene="dmsC"
                     /locus_tag="ACHOSC_RS01780"
                     /old_locus_tag="pgaptmp_000356"
     CDS             complement(9674..10537)
                     /gene="dmsC"
                     /locus_tag="ACHOSC_RS01780"
                     /old_locus_tag="pgaptmp_000356"
                     /EC_number="1.8.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dimethyl sulfoxide reductase anchor subunit
                     DmsC"
                     /protein_id="HGU4546370.1"
                     /translation="MGSGWHEWPLMIFTVFGQCVAGGFIVLALALLKGDLRAEAQQRV
                     IACMFGLWVLMGIGFIASMLHLGSPMRAFNSLNRVGASALSNEIASGSIFFAVGGIGW
                     LLAMLKKLSPALRTLWLIVTMVLGVIFVWMMVRVYNSIDTVPTWYSIWTPMGFFLTMF
                     MGGPLLGYLLLSLAGVDGWAMRLLPAISVLALVVSGVMSVMQGTELATIHSSVQQAAA
                     LVPDYGALMSWRIVLLAVALCLWIAPQLKGYQPAVPLLSVSFILLLAGELIGRGVFYG
                     LHMTVGMAVAS"
     gene            complement(10539..11156)
                     /gene="dmsB"
                     /locus_tag="ACHOSC_RS01785"
                     /old_locus_tag="pgaptmp_000357"
     CDS             complement(10539..11156)
                     /gene="dmsB"
                     /locus_tag="ACHOSC_RS01785"
                     /old_locus_tag="pgaptmp_000357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000213046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dimethylsulfoxide reductase iron-sulfur subunit
                     DmsB"
                     /protein_id="HGU4546371.1"
                     /translation="MTTQYGFFIDSSRCTGCKTCELACKDYKDLTPEVSFRRIYEYAG
                     GDWQEDNGVWHQNVFAYYLSISCNHCEDPACTKVCPSGAMHKREDGFVVVDEDVCIGC
                     RYCHMACPYGAPQYNETKGHMTKCDGCYDRVAEGKKPICVESCPLRALDFGPIDELRK
                     KHGDLAAVAPLPRAHFTKPNIVIKPNANSRPTGDTTGYLANPKEV"
     gene            complement(11167..13611)
                     /gene="dmsA"
                     /locus_tag="ACHOSC_RS01790"
                     /old_locus_tag="pgaptmp_000358"
     CDS             complement(11167..13611)
                     /gene="dmsA"
                     /locus_tag="ACHOSC_RS01790"
                     /old_locus_tag="pgaptmp_000358"
                     /EC_number="1.8.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415414.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009389 - dimethyl sulfoxide reductase
                     activity [Evidence IEA];
                     GO_function: GO:0030151 - molybdenum ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dimethylsulfoxide reductase subunit A"
                     /protein_id="HGU4546372.1"
                     /translation="MKTKIPDAVLAAEVSRRGLVKTTAIGGLAMASSALTLPFSRIAH
                     AVDSAIPTKSDEKVIWSACTVNCGSRCPLRMHVVDGEIKYVETDNTGDDNYDGLHQVR
                     ACLRGRSMRRRVYNPDRLKYPMKRVGARGEGKFERISWEEAYDIIATNMQRLIKEYGN
                     ESIYLNYGTGTLGGTMTRSWPPGNTLVARLMNCCGGYLNHYGDYSSAQIAEGLNYTYG
                     GWADGNSPSDIENSKLVVLFGNNPGETRMSGGGVTYYLEQARQKSNARMIIIDPRYTD
                     TGAGREDEWIPIRPGTDAALVNGLAYVMITENLVDQAFLDKYCVGYDEKTLPASAPKN
                     GHYKAYILGEGPDGVAKTPEWASQITGVPADKIIKLAREIGSTKPAFISQGWGPQRHA
                     NGEIATRAISMLAILTGNVGINGGNSGAREGSYSLPFVRMPTLENPIQTSISMFMWTD
                     AIERGPEMTALRDGVRGKDKLDVPIKMIWNYAGNCLINQHSEINRTHEILQDDKKCEL
                     IVVIDCHMTSSAKYADILLPDCTASEQMDFALDASCGNMSYVIFNDQVIKPRFECKTI
                     YEMTSELAKRLGVEQQFTEGRTQEEWMRHLYAQSREAIPELPTFEEFRKQGIFKKRDP
                     QGHHVAYKAFREDPQANPLTTPSGKIEIYSQALADIAATWELPEGDVIDPLPIYTPGF
                     ESYQDPLNKQYPLQLTGFHYKSRVHSTYGNVDVLKAACRQEMWINPLDAQKRGINNGD
                     KVRIFNDRGEVHIEAKVTPRMMPGVVALGEGAWYDPDTKRVDKGGCINVLTTQRPSPL
                     AKGNPSHTNLVQVEKV"
     gene            complement(13850..15142)
                     /gene="serS"
                     /locus_tag="ACHOSC_RS01795"
                     /old_locus_tag="pgaptmp_000359"
     CDS             complement(13850..15142)
                     /gene="serS"
                     /locus_tag="ACHOSC_RS01795"
                     /old_locus_tag="pgaptmp_000359"
                     /EC_number="6.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000887397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004828 - serine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006434 - seryl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine--tRNA ligase"
                     /protein_id="HGU4546373.1"
                     /translation="MLDPNLLRNEPDAVAEKLARRGFKLDVDKLGALEERRKVLQVKT
                     ENLQAERNSRSKSIGQAKARGEDIEPLRLEVNKLGEELDAAKAELDALQAEIRDIALT
                     IPNLPADEVPVGKDENDNVEVSRWGTPREFDFEVRDHVTLGEMHSGLDFAAAVKLTGS
                     RFVVMKGQIARMHRALSQFMLDLHTEQHGYSENYVPYLVNQDTLYGTGQLPKFAGDLF
                     HTRPLEEEADTSNYALIPTAEVPLTNLVRGEIIDEDDLPIKMTAHTPCFRSEAGSYGR
                     DTRGLIRMHQFDKVEMVQIVRPEDSMAALEEMTGHAEKVLQLLGLPYRKIILCTGDMG
                     FGACKTYDLEVWIPAQNTYREISSCSNVWDFQARRMQARCRSKSDKKTRLVHTLNGSG
                     LAVGRTLVAVMENYQQADGRIEVPEVLRPYMNGLEYIG"
     gene            complement(15233..16576)
                     /gene="rarA"
                     /locus_tag="ACHOSC_RS01800"
                     /old_locus_tag="pgaptmp_000360"
                     /gene_synonym="mgsA"
     CDS             complement(15233..16576)
                     /gene="rarA"
                     /locus_tag="ACHOSC_RS01800"
                     /old_locus_tag="pgaptmp_000360"
                     /gene_synonym="mgsA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication-associated recombination protein
                     RarA"
                     /protein_id="HGU4546374.1"
                     /translation="MSNLSLDFSDNTFQPLAARMRPENLAQYIGQQHLLAAGKPLPRA
                     IEAGHLHSMILWGPPGTGKTTLAEVIARYANADVERISAVTSGVKEIREAIERARQNR
                     NAGRRTILFVDEVHRFNKSQQDAFLPHIEDGTITFIGATTENPSFELNSALLSRARVY
                     LLKSLGTEDIEQVLTQAMEDKTRGYGGQDIVLPDETRRAIAELVNGDARRALNTLEMM
                     ADMAEVNDSGKRVLKPELLTEIAGERSARFDNKGDRFYDLISALHKSVRGSAPDAALY
                     WYARIITAGGDPLYVARRCLAIASEDVGNADPRAMQVAIAAWDCFTRVGPAEGERAIA
                     QAIVYLACAPKSNAVYTAFKAALADARERPDYDVPVHLRNAPTKLMKEMGYGQEYRYA
                     HDEANAYAAGEVYFPPEIAQTRYYFPTNRGLEGKIGEKLAWLAEQDQNSPIKRYR"
     gene            complement(16587..17198)
                     /gene="lolA"
                     /locus_tag="ACHOSC_RS01805"
                     /old_locus_tag="pgaptmp_000361"
     CDS             complement(16587..17198)
                     /gene="lolA"
                     /locus_tag="ACHOSC_RS01805"
                     /old_locus_tag="pgaptmp_000361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010354356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA];
                     GO_process: GO:0042953 - lipoprotein transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipoprotein chaperone LolA"
                     /protein_id="HGU4546375.1"
                     /translation="MKKIAITCALLSSLVASSVWADAASDLKSRLDKVSSFHASFTQK
                     VTDGSGAAVQEGQGDLWVKRPNLFNWHMTQPDESILVSDGKTLWFYNPFVEQATATWL
                     KDATGNTPFMLIARNQSSDWQQYNIKQNGDDFVLTPKASNGNLKQFTINVGRDGTIHQ
                     FSAVEQDDQRSSYQLKSQQNGAVDAAKFTFTPPQGVTVDDQRK"
     gene            complement(17353..21459)
                     /gene="ftsK"
                     /locus_tag="ACHOSC_RS01810"
                     /old_locus_tag="pgaptmp_000362"
     CDS             complement(17353..21459)
                     /gene="ftsK"
                     /locus_tag="ACHOSC_RS01810"
                     /old_locus_tag="pgaptmp_000362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA translocase FtsK"
                     /protein_id="HGU4546376.1"
                     /translation="MSQEYTEDKEVTLTKLSSGRRLLEALLILIVLFAVWLMAALLSF
                     NPSDPSWSQTAWHEPIHNLGGMPGAWLADTLFFIFGVMAYTIPVIIVGGCWFAWRHQS
                     SDEYIDYFAVSLRIIGVLALILTSCGLAAINADDIWYFASGGVIGSLLSTTLQPLLHS
                     SGGTIALLCVWAAGLTLFTGWSWVTIAEKLGGWILNILTFASNRTRRDDTWVDEDEYE
                     DDEEYEDENHGKQHESRRARILRGALARRKRLAEKFINPMGRQTDAALFSGKRMDDDE
                     EITYTARGVAADPDDVLFSGNRATQPEYDEYDPLLNGAPITEPVAVAAAATTATQSWA
                     APVEPVTQTPPVASVDVPPSQPTVAWQPVPGPQTGEPVIAPAPEGYPQQSQYAQPAVQ
                     YNEPLQQPVQPQQSYYAPAAEQPAQQPYYAPAAEQPVQQPYYATAPEQPAQQPYYAPA
                     PEQPVAGNAWQAEEQQSTFAPQSTYQTEQTYQQPAAQEPLYQQPQSVEQQPVVEPEPV
                     VEETKPARPPLYYFEEVEEKRAREREQLAAWYQPIPEPVKEPEPIKSSLKAPSVAAVP
                     PVEAAAAVSPLASGVKKATLATGAAATVAAPVFSLANSGGPRPQVKEGIGPQLPRPKR
                     IRVPTRRELASYGIKLPSQRAAEEKAREAQRNQYDSGDQYNDDEIDAMQQDELARQFA
                     QTQQQRYGEQYQHDVPVNAEDADAAAEAELARQFAQTQQQRYSGEQPAGANPFSLDDF
                     EFSPMKALLDDGPHEPLFTPIVEPVQQPQQPVAPQQQYQQPQQPVPPQQQYQQPQQPV
                     APQPQYQQPQQQVAPQPQYQQPQQPVAPQPQYQQPQQPVAPQPQYQQPQQPVAPQQQD
                     TLLHPLLMRNGDSRPLHKPTTPLPSLDLLTPPPSEVEPVDTFALEQMARLVEARLADF
                     RIKADVVNYSPGPVITRFELNLAPGVKAARISNLSRDLARSLSTVAVRVVEVIPGKPY
                     VGLELPNKKRQTVYLREVLDNAKFRDNPSPLTVVLGKDIAGEPVVADLAKMPHLLVAG
                     TTGSGKSVGVNAMILSMLYKAQPEDVRFIMIDPKMLELSVYEGIPHLLTEVVTDMKDA
                     ANALRWCVNEMERRYKLMSALGVRNLAGYNEKIAEADRMMRPIPDPYWKPGDSMDAQH
                     PVLKKEPYIVVLVDEFADLMMTVGKKVEELIARLAQKARAAGIHLVLATQRPSVDVIT
                     GLIKANIPTRIAFTVSSKIDSRTILDQAGAESLLGMGDMLYSGPNSTLPVRVHGAFVR
                     DQEVHAVVQDWKARGRPQYVDGITSDSESEGGAGGFDGAEELDPLFDQAVQFVTEKRK
                     ASISGVQRQFRIGYNRAARIIEQMEAQGIVSEQGHNGNREVLAPPPFD"
     gene            complement(21594..22088)
                     /gene="lrp"
                     /locus_tag="ACHOSC_RS01815"
                     /old_locus_tag="pgaptmp_000363"
     CDS             complement(21594..22088)
                     /gene="lrp"
                     /locus_tag="ACHOSC_RS01815"
                     /old_locus_tag="pgaptmp_000363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001591745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucine-responsive transcriptional regulator
                     Lrp"
                     /protein_id="HGU4546377.1"
                     /translation="MVDSKKRPGKDLDRIDRNILNELQKDGRISNVELSKRVGLSPTP
                     CLERVRRLERQGFIQGYTALLNPHYLDASLLVFVEITLNRGAPDVFEQFNTAVQKLEE
                     IQECHLVSGDFDYLLKTRVPDMSAYRKLLGETLLRLPGVNDTRTYVVMEEVKQSNRLV
                     IKTR"
     gene            22632..23597
                     /gene="trxB"
                     /locus_tag="ACHOSC_RS01820"
                     /old_locus_tag="pgaptmp_000364"
     CDS             22632..23597
                     /gene="trxB"
                     /locus_tag="ACHOSC_RS01820"
                     /old_locus_tag="pgaptmp_000364"
                     /EC_number="1.8.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004791 - thioredoxin-disulfide reductase
                     (NADPH) activity [Evidence IEA];
                     GO_process: GO:0019430 - removal of superoxide radicals
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-disulfide reductase"
                     /protein_id="HGU4546378.1"
                     /translation="MGTTKHSKLLILGSGPAGYTAAVYAARANLQPVLITGMEKGGQL
                     TTTTEVENWPGDPNDLTGPLLMERMHEHATKFETEIIFDHINKVDLQNRPFRLNGDNG
                     EYTCDALIIATGASARYLGLPSEEAFKGSGVSACATCDGFFYRNQKVAVIGGGNTAVE
                     EALYLSNIASEVHLIHRRDGFRAEKILIKRLMDKVENGNIILHTNRTLEEVTGDQMGV
                     TGVRLRDTQNSDNIESLDVAGLFVAIGHSPNTAIFEGQLELENGYIKVQSGIHGNATQ
                     TSIPGVFAAGDVMDHIYRQAITSAGTGCMAALDAERYLDGLADAK"
     gene            23720..25486
                     /gene="cydD"
                     /locus_tag="ACHOSC_RS01825"
                     /old_locus_tag="pgaptmp_000365"
     CDS             23720..25486
                     /gene="cydD"
                     /locus_tag="ACHOSC_RS01825"
                     /old_locus_tag="pgaptmp_000365"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0042883 - cysteine transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine/glutathione ABC transporter
                     permease/ATP-binding protein CydD"
                     /protein_id="HGU4546379.1"
                     /translation="MNKSRQKELTRWLKQQSVISQRWLNISRLLGFVSGILIIAQAWF
                     MARILQHMIMENIPREALLLPFTLLVLTFVLRAWVVWLRERVGYHAGQHIRFAIRRQV
                     LDRLQQAGPAWIQGKPAGSWATLVLEQIDDMHDYYARYLPQMALAVSVPLLIVVAIFP
                     SNWAAALILLGTAPLIPLFMALVGMGAADANRRNFLALARLSGHFLDRLRGMETLRIF
                     GRGEAEIESIRSASEDFRQRTMEVLRLAFLSSGILEFFTSLSIALVAVYFGFSYLGEL
                     DFGHYDTGVTLAAGFLALILAPEFFQPLRDLGTFYHAKAQAVGAADSLKTFMETPLAH
                     PQRGEVELASTDPVTIEAEDLFITSPEGKTLAGPLNFTLPAGQRAVLVGRSGSGKSSL
                     LNALSGFLSYQGSLRINGIELRDLSPESWRKHLSWVGQNPQLPAATLRDNVLLARPDA
                     SEQELQAALDNAWVSEFLPLLPQGVDTPVGDQAARLSVGQAQRVAVARALLNPCSLLL
                     LDEPAASLDAHSEQRVMEALNAASLRQTTLMVTHQLEDLADWDVIWVMQDGRIIEQGR
                     YAELSVAGGPFATLLAHRQEEI"
     gene            25487..27208
                     /gene="cydC"
                     /locus_tag="ACHOSC_RS01830"
                     /old_locus_tag="pgaptmp_000366"
     CDS             25487..27208
                     /gene="cydC"
                     /locus_tag="ACHOSC_RS01830"
                     /old_locus_tag="pgaptmp_000366"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0034775 - glutathione transmembrane
                     transport [Evidence IEA];
                     GO_process: GO:0045454 - cell redox homeostasis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine/glutathione ABC transporter ATP-binding
                     protein/permease CydC"
                     /protein_id="HGU4546380.1"
                     /translation="MRALLPYLALYKRHKWMLSLGIVLAIVTLLASIGLLTLSGWFLS
                     ASAVAGVAGLYSFNYMLPAAGVRGAAITRTAGRYFERLVSHDATFRVLQHLRIYTFSK
                     LLPLSPAGLARYRQGELLNRVVADVDTLDHLYLRVISPLVGAFVVIMVVTIGLSFLDF
                     TLAFRLGGIMLLTLFLMPPLFYRAGKSTGQNLTHLRGQYRQQLTAWLQGQAELTIFGA
                     SDRYRTQLENTEIQWLEAQRRQSELTALSQAIMLLIGALAVILMLWMASGGVGGNAQP
                     GALIALFVFCALAAFEALAPVTGAFQHLGQVIASAVRITDLTDQKPEVTFPDTQTRVA
                     DRVSLTLRDVQFTYPEQSQQALKGISLQVNAGEHIAILGRTGCGKSTLLQLLTRAWDP
                     QQGEILLNDSPIASLNEAALRQTISVVPQRVHLFSATLRDNLLLASPGSSDEALAESL
                     RRVGLEKLLEDAGLNSWLGEGGRQLSGGELRRLAIARALLHDAPLVLLDEPTEGLDAT
                     TESQILELLAEMMREKTVLMVTHRLRGLSRFQQIIVMDNGQIIEQGTHAELLARQGRY
                     YQFKQGL"
     gene            27250..27954
                     /gene="aat"
                     /locus_tag="ACHOSC_RS01835"
                     /old_locus_tag="pgaptmp_000367"
     CDS             27250..27954
                     /gene="aat"
                     /locus_tag="ACHOSC_RS01835"
                     /old_locus_tag="pgaptmp_000367"
                     /EC_number="2.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008914 - leucyl-tRNA--protein transferase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="leucyl/phenylalanyl-tRNA--protein transferase"
                     /protein_id="HGU4546381.1"
                     /translation="MRLVQLSRHSIAFPSPEGALREPNGLLALGGDLSPARLLMAYQR
                     GIFPWFSPGDPILWWSPDPRAVLWPESLHISRSMKRFHKRSPYRVTMNYAFGQVIEGC
                     ASDREEGTWITRGVVEAYHRLHELGHAHSIEVWREDELVGGMYGVAQGTLFCGESMFS
                     RMENASKTALLVFCEEFIGHGGKLIDCQVLNDHTASLGACEIPRRDYLNYLNQMRLGR
                     LPNNFWVPRCLFSPQE"
     gene            28239..28457
                     /gene="infA"
                     /locus_tag="ACHOSC_RS01840"
                     /old_locus_tag="pgaptmp_000368"
     CDS             28239..28457
                     /gene="infA"
                     /locus_tag="ACHOSC_RS01840"
                     /old_locus_tag="pgaptmp_000368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001040186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-1"
                     /protein_id="HGU4546382.1"
                     /translation="MAKEDNIEMQGTVLETLPNTMFRVELENGHVVTAHISGKMRKNY
                     IRILTGDKVTVELTPYDLSKGRIVFRSR"
     gene            28711..28798
                     /locus_tag="ACHOSC_RS01845"
                     /old_locus_tag="pgaptmp_000369"
     tRNA            28711..28798
                     /locus_tag="ACHOSC_RS01845"
                     /old_locus_tag="pgaptmp_000369"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:28745..28747,aa:Ser,seq:gga)
     gene            complement(29141..31417)
                     /gene="clpA"
                     /locus_tag="ACHOSC_RS01850"
                     /old_locus_tag="pgaptmp_000370"
     CDS             complement(29141..31417)
                     /gene="clpA"
                     /locus_tag="ACHOSC_RS01850"
                     /old_locus_tag="pgaptmp_000370"
                     /EC_number="3.4.21.92"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0043335 - protein unfolding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit
                     ClpA"
                     /protein_id="HGU4546383.1"
                     /translation="MLNQELELSLNMAFARAREHRHEFMTVEHLLLALLSNPSAREAL
                     EACSVDLVALRQELEAFIEQTTPVLPASEEERDTQPTLSFQRVLQRAVFHVQSSGRNE
                     VTGANVLVAIFSEQESQAAYLLRKHEVSRLDVVNFISHGTRKDEPTQSSDPGSQPNSE
                     EQAGGEERMENFTTNLNQLARVGGIDPLIGREKELERAIQVLCRRRKNNPLLVGESGV
                     GKTAIAEGLAWRIVQGDVPEVMADCTIYSLDIGSLLAGTKYRGDFEKRFKALLKQLEQ
                     DTNSILFIDEIHTIIGAGAASGGQVDAANLIKPLLSSGKIRVIGSTTYQEFSNIFEKD
                     RALARRFQKIDITEPSIEETVQIINGLKPKYEAHHDVRYTAKAVRAAVELAVKYINDR
                     HLPDKAIDVIDEAGARARLMPVSKRKKTVNVADIESVVARIARIPEKSVSQSDRDTLK
                     NLGDRLKMLVFGQDKAIEALTEAIKMARAGLGHEHKPVGSFLFAGPTGVGKTEVTVQL
                     SKALGIELLRFDMSEYMERHTVSRLIGAPPGYVGFDQGGLLTDAVIKHPHAVLLLDEI
                     EKAHPDVFNILLQVMDNGTLTDNNGRKADFRNVVLVMTTNAGVRETERKSIGLIHQDN
                     STDAMEEIKKIFTPEFRNRLDNIIWFDHLSTDVIHQVVDKFIVELQVQLDQKGVSLEV
                     SQEARNWLAEKGYDRAMGARPMARVIQDNLKKPLANELLFGSLVDGGQVTVALDKEKN
                     ELTYGFQSAQKHKAEAAH"
     gene            complement(31448..31768)
                     /gene="clpS"
                     /locus_tag="ACHOSC_RS01855"
                     /old_locus_tag="pgaptmp_000371"
     CDS             complement(31448..31768)
                     /gene="clpS"
                     /locus_tag="ACHOSC_RS01855"
                     /old_locus_tag="pgaptmp_000371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005128368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease adapter ClpS"
                     /protein_id="HGU4546384.1"
                     /translation="MGKTNDWLDFDQLAEEKVRDALKPPSMYKVILVNDDYTPMEFVI
                     DVLQKFFSYDVERATQLMLAVHYQGKAICGVFTAEVAETKVAMVNKYARENEHPLLCT
                     LEKA"
     gene            complement(31867..31941)
                     /gene="yljB"
                     /locus_tag="ACHOSC_RS01860"
                     /old_locus_tag="pgaptmp_000372"
     CDS             complement(31867..31941)
                     /gene="yljB"
                     /locus_tag="ACHOSC_RS01860"
                     /old_locus_tag="pgaptmp_000372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YljB"
                     /protein_id="HGU4546385.1"
                     /translation="MNQKFEAVNAIDRNVTDVADANDR"
     gene            32091..32315
                     /gene="cspD"
                     /locus_tag="ACHOSC_RS01865"
                     /old_locus_tag="pgaptmp_000373"
     CDS             32091..32315
                     /gene="cspD"
                     /locus_tag="ACHOSC_RS01865"
                     /old_locus_tag="pgaptmp_000373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold shock-like protein CspD"
                     /protein_id="HGU4546386.1"
                     /translation="MEKGTVKWFNNAKGFGFICPEGGGEDIFAHYSTIQMDGYRTLKA
                     GQSVQFDVHQGPKGNHASVIVPVEVEAAVA"
     gene            complement(32388..34334)
                     /gene="macB"
                     /locus_tag="ACHOSC_RS01870"
                     /old_locus_tag="pgaptmp_000374"
     CDS             complement(32388..34334)
                     /gene="macB"
                     /locus_tag="ACHOSC_RS01870"
                     /old_locus_tag="pgaptmp_000374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1990195 - macrolide transmembrane
                     transporter complex [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="macrolide ABC transporter ATP-binding
                     protein/permease MacB"
                     /protein_id="HGU4546387.1"
                     /translation="MTPLLELKDIRRSYPAGDEQVEVLKGITLDIYAGEMVAIVGASG
                     SGKSTLMNILGCLDKATSGTYRVAGQDVATLDADALAQLRREHFGFIFQRYHLLSHLT
                     AEQNVEVPAVYAGLERKQRLLRAQELLQRLGLEDRTEYYPAQLSGGQQQRVSIARALM
                     NGGQVILADEPTGALDSHSGEEVMAILHQLRDRGHTVIIVTHDPQVAAQAERVIEIRD
                     GEIVRNPPAIEKVNVAGGTEPVVNTVSGWRQFVSGFNEALTMAWRALAANKMRTLLTM
                     LGIIIGIASVVSIVVVGDAAKQMVLADIRSIGTNTIDVYPGKDFGDDDPQYQQALKYD
                     DLIAIQKQPWVASATPAVSQNLRLRYNNVDVAASANGVSGDYFNVYGMTFSEGNTFNQ
                     EQLNGRAQVVVLDSNTRRQLFPHKADVVGEVILVGNMPARVIGVAEEKQSMFGSSKVL
                     RVWLPYSTMSGRVMGQSWLNSITVRVKEGFDSAEAEQQLTRLLSLRHGKKDFFTWNMD
                     GVLKTVEKTTRTLQLFLTLVAVISLVVGGIGVMNIMLVSVTERTREIGIRMAVGARAS
                     DVLQQFLIEAVLVCLVGGALGITLSLLIAFTLQLFLPGWEIGFSPLALLLAFLCSTVT
                     GILFGWLPARNAARLDPVDALARE"
     gene            complement(34331..35446)
                     /gene="macA"
                     /locus_tag="ACHOSC_RS01875"
                     /old_locus_tag="pgaptmp_000375"
     CDS             complement(34331..35446)
                     /gene="macA"
                     /locus_tag="ACHOSC_RS01875"
                     /old_locus_tag="pgaptmp_000375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308991.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019898 - extrinsic component of membrane
                     [Evidence IEA];
                     GO_component: GO:1990195 - macrolide transmembrane
                     transporter complex [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:1990961 - xenobiotic detoxification by
                     transmembrane export across the plasma membrane [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="macrolide transporter subunit MacA"
                     /protein_id="HGU4546388.1"
                     /translation="MKKRKTVKKRYVIALVIVIAGLITLWRILNAPVPTYQTLIVRPG
                     DLQQSVLATGKLDALRKVDVGAQVSGQLKTLSVAIGDKVKKDQLLGVIDPEQAENQIK
                     EVEATLMELRAQRQQAEAELKLARVTYSRQQRLAQTQAVSQQDLDTAATEMAVKQAQI
                     GTIDAQIKRNQASLDTAKTNLDYTRIVAPMAGEVTQITTLQGQTVIAAQQAPNILTLA
                     DMSTMLVKAQVSEADVIHLKPGQKAWFTVLGDPLTRYEGQIKDVLPTPEKVNDAIFYY
                     ARFEVPNPNGLLRLDMTAQVHIQLTDVKNVLTIPLSALGDPVGDNRYKVKLLRNGETR
                     EREVTIGARNDTDVEIVKGLEAGDEVVIGEAKPGAAQ"
     gene            35597..36553
                     /gene="ybjX"
                     /locus_tag="ACHOSC_RS01880"
                     /old_locus_tag="pgaptmp_000376"
     CDS             35597..36553
                     /gene="ybjX"
                     /locus_tag="ACHOSC_RS01880"
                     /old_locus_tag="pgaptmp_000376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001322270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VirK/YbjX family protein"
                     /protein_id="HGU4546389.1"
                     /translation="MNMSQLTERTFTPSESLSSLSLFLSLARGQCRPGKFWHRRSFRQ
                     KFLLRSLIMPRLSVEWMNELSHWPNLNVLLTRQPRLPVRLHRPYLAANLSRKQLLEAL
                     RYHYALLRGCMSAEEFSLYLNTPGLQLAKLEGKNGEQFTLELTMMISMDKEGDSTILF
                     RNSEGIPLAELTFTLCEYQGKRTMFIGGLQGAKWEIPHQEIQNATKACHGLFPKRLVM
                     EAACLFAQRLQVEQIIAVSNETHIYRSLRYRDKEGKIHADYNAFWESVGGVCDAERHY
                     RLPAQIARKEIAEIASKKRAEYRRRYEMLDAIQPQMATMFRG"
     gene            complement(36550..38208)
                     /gene="ybjD"
                     /locus_tag="ACHOSC_RS01885"
                     /old_locus_tag="pgaptmp_000377"
     CDS             complement(36550..38208)
                     /gene="ybjD"
                     /locus_tag="ACHOSC_RS01885"
                     /old_locus_tag="pgaptmp_000377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent endonuclease"
                     /protein_id="HGU4546390.1"
                     /translation="MILERVEIVGFRGINRLSLMLEQNNVLIGENAWGKSSLLDALTL
                     LLSPESDLYHFERDDFWFPPGDINGREHHLHIILTFRESLPGRHRVRRYRPLEACWTP
                     CTDGYHRIFYRLEGESAEDGSVMTLRSFLDKDGHPIDVEDINDQARHLVRLMPVLRLR
                     DARFMRRIRNGTVPNVPNVEVTARQLDFLARELSSHPQNLSDGQIRQGLSAMVQLLEH
                     YFSEQGAGQARYRLMRRRASNEQRSWRYLDIINRMIDRPGGRSYRVILLGLFATLLQA
                     KGTLRLDKDARPLLLIEDPETRLHPIMLSVAWHLLNLLPLQRIATTNSGELLSLTPVE
                     HVCRLVRESSRVAAWRLGPSGLSTEDSRRISFHIRFNRPSSLFARCWLLVEGETETWV
                     INELARQCGHHFDAEGIKVIEFAQSGLKPLVKFARRMGIEWHVLVDGDEAGKKYAATV
                     RSLLNNDREAEREHLTALPALDMEHFMYRQGFSDVFHRVAQIPENVPMNLRKIISKAI
                     HSSSKPDLAIEVAMEAGRRGVDSVPTLLKKMFSRVLWLARGRAD"
     gene            38634..39329
                     /gene="aqpZ"
                     /locus_tag="ACHOSC_RS01890"
                     /old_locus_tag="pgaptmp_000378"
     CDS             38634..39329
                     /gene="aqpZ"
                     /locus_tag="ACHOSC_RS01890"
                     /old_locus_tag="pgaptmp_000378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015250 - water channel activity [Evidence
                     IEA];
                     GO_process: GO:0006833 - water transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aquaporin Z"
                     /protein_id="HGU4546391.1"
                     /translation="MFRKLAAECFGTFWLVFGGCGSAVLAAGFPELGIGFAGVALAFG
                     LTVLTMAFAVGHISGGHFNPAVTIGLWAGGRFPAKEVVGYVIAQVVGGIVAAALLYLI
                     ASGKTGFDAAASGFASNGYGEHSPGGYSMLSALVVELVLSAGFLLVIHGATDKFAPAG
                     FAPIAIGLALTLIHLISIPVTNTSVNPARSTAVAIFQGGWALEQLWFFWVVPIVGGII
                     GGLIYRTLLEKRD"
     gene            39824..40723
                     /gene="lysO"
                     /locus_tag="ACHOSC_RS01895"
                     /old_locus_tag="pgaptmp_000379"
     CDS             39824..40723
                     /gene="lysO"
                     /locus_tag="ACHOSC_RS01895"
                     /old_locus_tag="pgaptmp_000379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415395.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-lysine exporter LysO"
                     /protein_id="HGU4546392.1"
                     /translation="MFSGLLIILVPLIVGYLIPLRQQAALKVINQLLSWMVYLILFFM
                     GISLAFLDNLASNLLAILHYSAVSITVILLCNIAALMWLERGLPWRNHHQQEKLPSRI
                     AMALESLKLCGVVVIGFAIGLSGLAFLQHATEASEYTLILLLFLVGIQLRNNGMTLKQ
                     IVLNRRGMIVAVVVVVSSLIGGLINAFILDLPINTSLAMASGFGWYSLSGILLTESFG
                     PVIGSAAFFNDLARELIAIMLIPGLIRRSRSTALGLCGATSMDFTLPVLQRTGGLDMV
                     PAAIVHGFILSLLVPILIAFFSA"
     gene            40867..42519
                     /gene="hcp"
                     /locus_tag="ACHOSC_RS01900"
                     /old_locus_tag="pgaptmp_000380"
                     /gene_synonym="priS"
     CDS             40867..42519
                     /gene="hcp"
                     /locus_tag="ACHOSC_RS01900"
                     /old_locus_tag="pgaptmp_000380"
                     /gene_synonym="priS"
                     /EC_number="1.7.99.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005122080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016661 - oxidoreductase activity, acting
                     on other nitrogenous compounds as donors [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxylamine reductase"
                     /protein_id="HGU4546393.1"
                     /translation="MFCVQCEQTIRTPAGNGCSYAQGMCGKTAETSDLQDLLIAALQG
                     LSAWAVKAREYGIINHDVDSFAPRAFFSTLTNVNFDSPRIVGYAREAIALREALKAQC
                     LAVDANARVDNPMADLQLVSDDLGELQRQAAEFTPNKDKAAIGENILGLRLLCLYGLK
                     GAAAYMEHAHVLGQYDNDIYAQYHKIMAWLGTWPADMNALLECSMEIGQMNFKVMSIL
                     DAGETGKYGHPTPTQVNVKATAGKCILISGHDLKDLYNLLEQTEGTGVNVYTHGEMLP
                     AHGYPELRKFKHLVGNYGSGWQNQQVEFARFPGPIVMTSNCIIDPTVGAYDDRIWTRS
                     IVGWPGVRHLDGDDFSAVITQAQQMAGFPYSEIPHLITVGFGRQTLLGAADTLIDLVS
                     REKLRHIFLLGGCDGARGERHYFTDFATSVPDDCLILTLACGKYRFNKLEFGDIEGLP
                     RLVDAGQCNDAYSAIILAVTLAEKLGCGVNDLPLSLVLSWFEQKAIVILLTLLSLGVK
                     NIVTGPTAPGFLTPDLLAVLNEKFGLRSITTVEEDMKQLLSA"
     gene            42531..43499
                     /gene="hcr"
                     /locus_tag="ACHOSC_RS01905"
                     /old_locus_tag="pgaptmp_000381"
     CDS             42531..43499
                     /gene="hcr"
                     /locus_tag="ACHOSC_RS01905"
                     /old_locus_tag="pgaptmp_000381"
                     /EC_number="1.6.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH oxidoreductase"
                     /protein_id="HGU4546394.1"
                     /translation="MTMPTNQCPWRMQVHHITQETPDVWTISLICHDYYPYRAGQYAL
                     VSVRNSAETLRAYTISSTPGVSEYITLTVRRIDDGVGSQWLTRDVKRGDYLWLSDAMG
                     EFTCDDKAEDKFLLLAAGCGVTPIMSMRRWLAKNRPQADVRVIYNVRTPQDVIFADEW
                     RNYPVTLVAENNVTEGFIAGRLTRELLAGVPDLASRTVMTCGPAPYMDWVEQEVKALG
                     VTRFFKEKFFTPVAEAATSGLKFTKLQPAREFYAPVGTTLLEALESNNVPVVAACRAG
                     VCGCCKTKVVSGEYTVSSTMTLTDAEIAEGYVLACSCHPQGDLVLA"
     gene            43632..45350
                     /gene="poxB"
                     /locus_tag="ACHOSC_RS01910"
                     /old_locus_tag="pgaptmp_000382"
     CDS             43632..45350
                     /gene="poxB"
                     /locus_tag="ACHOSC_RS01910"
                     /old_locus_tag="pgaptmp_000382"
                     /EC_number="1.2.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_function: GO:0052737 - pyruvate dehydrogenase (quinone)
                     activity [Evidence IEA];
                     GO_process: GO:0006090 - pyruvate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ubiquinone-dependent pyruvate dehydrogenase"
                     /protein_id="HGU4546395.1"
                     /translation="MKQTVAAYIAKTLESAGVKRIWGVTGDSLNGLSDSLNRMGTIEW
                     MSTRHEEVAAFAAGAEAQLSGELAVCAGSCGPGNLHLINGLFDCHRNHVPVLAIAAHI
                     PSSEIGSGYFQETHPQELFRECSHYCELVSSPEQIPQVLAIAMRKAVLNRGVSVVVLP
                     GDVALKPAPEGATTHWYHAPQPVVTPEEEELRKLAQLLRYSSNIALMCGSGCAGAHKE
                     LVEFAGKIKAPIVHALRGKEHVEYDNPYDVGMTGLIGFSSGFHTMMNADTLVLLGTQF
                     PYRAFYPTDAKIIQIDINPASIGAHSKVDMALVGDIKSTLRALLPLVEEKADRKFLDK
                     ALEDYRDARKGLDDLAKPSEKAIHPQYLAQQISHFAADDAIFTCDVGTPTVWAARYLK
                     MNGKRRLLGSFNHGSMANAMPQALGAQATEPERQVVAMCGDGGFSMLMGDFLSVVQMK
                     LPVKIVVFNNSVLGFVAMEMKAGGYLTDGTELHDTNFARIAEACGITGIRVEKASEVD
                     EALQRAFSIDGPVLVDVVVAKEELAIPPQIKLEQAKGFSLYMLRAIISGRGDEVIELA
                     KTNWLR"
     gene            45387..46388
                     /gene="ltaE"
                     /locus_tag="ACHOSC_RS01915"
                     /old_locus_tag="pgaptmp_000383"
     CDS             45387..46388
                     /gene="ltaE"
                     /locus_tag="ACHOSC_RS01915"
                     /old_locus_tag="pgaptmp_000383"
                     /EC_number="4.1.2.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="low-specificity L-threonine aldolase"
                     /protein_id="HGU4546396.1"
                     /translation="MIDLRSDTVTRPSRAMLEAMMAAPVGDDVYGDDPTVNALQDYAA
                     ELSGKEAAIFLPTGTQANLVALLSHCERGEEYIVGQAAHNYLFEAGGAAVLGSIQPQP
                     IDAAADGTLPLDKVAMKIKPDDIHFARTKLLSLENTHNGKVLPREYLKEAWEFTRERN
                     LALHVDGARIFNAVVAYGCELKEITQYCDSFTICLSKGLGTPVGSLLVGNRDYIKRAI
                     RWRKMAGGGMRQSGILAAAGMYALKNNVARLQEDHDNAAWMAEQLREAGADVMRQDTN
                     MLFVRVGEENAAALGEYMKARNVLINASPIVRLVTHLDVSREQLAEVAAHWRAFLAR"
     gene            46399..>47080
                     /locus_tag="ACHOSC_RS01920"
                     /old_locus_tag="pgaptmp_000384"
     CDS             46399..>47080
                     /locus_tag="ACHOSC_RS01920"
                     /old_locus_tag="pgaptmp_000384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415390.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-binding protein"
                     /protein_id="HGU4546397.1"
                     /translation="MPQRILVLGASGYIGQHLVRTLSQQGHQILAAARHVDRLAKLQL
                     ANVSCHKVDLSWPDNLPALLQDIDTVYFLVHSMGEGGDFIAQERQVALNVRDALREVP
                     VKQLIFLSSLQAPPHEQSDHLRARQATADILREANVPVTELRAGIIVGAGSAAFEVMR
                     DMVYNLPVLTPPRWVRSRTTPIALENLLHYLVALLDHPASEHRIFEAAGPEVLSYQQQ
                     FEHFMAVSG"
BASE COUNT        11278 a        12451 c        11704 g        11647 t
ORIGIN      
        1 agttacgggc ctataagcca ggcgagatat gatctatatc aatttctcat ctataatgct
       61 ttgttagtat ctcgtcgccg acttaataaa gagagagtta gtgtgaaagc tgacaaccct
      121 tttgatcttt tacttcctgc tgcaatggcc aaagtggccg aagaggcggg tgtctataaa
      181 gcaacgaaac atccgcttaa gactttctat ctggcgatta ccgccggtgt tttcatctca
      241 atcgcattcg tcttctatat cacagcaacc actggcacag gcacaatgcc cttcggcatg
      301 gcaaaactgg ttggcggcat ttgcttctct ctggggctga ttctttgtgt tgtctgcgga
      361 gccgatctct ttacttccac agtgttgatt gttgttgcta aggcgagtgg gcgcatcacc
      421 tggggtcagt tggcgaaaaa ctggctaaat gtctattttg gcaacctggt cggcgcactg
      481 ctgtttgtac ttttaatgtg gctttccggc gagtatatga ccgcaaatgg tcaatgggga
      541 ctaaacgtcc tacaaaccgc cgaccacaaa gtgcaccata cttttattga ggccgtctgc
      601 cttggtatcc tggcaaacct gatggtatgt ctggcagtat ggatgagtta ttctggccgt
      661 agcctgatgg acaaagcgtt cattatggtg ctgccggtcg cgatgtttgt tgccagcggt
      721 tttgagcaca gtatcgcaaa catgtttatg atcccgatgg gtattgtaat ccgcgacttc
      781 gcatctccgg aattctggac cgctgtcggt tctgcaccgg aaaatttttc tcacctgacc
      841 gtgatgaact tcatcactga taacctgatt ccggttacga tcggtaatat tatcggcggt
      901 ggtttgttgg ttgggttgac atactgggtc atttacctgc gtgaaaacga tcaccattaa
      961 tggttgtcga agtacgcagt aaataaaaaa tccacttaag aaggtaggtg ttacatgtcc
     1021 gagcttaatg aaaagttagc cacagcctgg gaaggtttta ccaaaggtga ctggcagaat
     1081 gaagtaaacg tccgtgactt cattcagaaa aactacactc cgtacgaggg tgacgagtcc
     1141 ttcctggctg gcgctactga agcgaccacc accctgtggg acaaagtaat ggaaggcgtt
     1201 aaactggaaa accgcactca cgcgccagtt gactttgaca ccgctgttgc ttccaccatc
     1261 acctctcacg acgctggcta catcaacaag cagcttgaga aaatcgttgg tctgcagact
     1321 gaagctccgc tgaaacgtgc tcttatcccg ttcggtggta tcaaaatgat cgaaggttcc
     1381 tgcaaagcgt acaaccgcga actggatccg atgatcaaaa aaatcttcac tgaataccgt
     1441 aaaactcaca accagggcgt gttcgacgtt tacactccgg acatcctgcg ttgccgtaaa
     1501 tctggtgttc tgaccggtct gccagatgca tatggccgtg gccgtatcat cggtgactac
     1561 cgtcgcgttg cgctgtacgg tatcgactac ctgatgaaag acaaactggc acagttcact
     1621 tctctgcagg ctgatctgga aaacggcgta aacctggaac agactatccg tctgcgcgaa
     1681 gaaatcgctg aacagcaccg cgctctgggt cagatgaaag aaatggctgc gaaatacggc
     1741 tacgacatct ctggtccggc taccaacgct caggaagcta tccagtggac ttacttcggc
     1801 tacctggctg ctgttaagtc tcagaacggt gctgcaatgt ccttcggtcg tacctccacc
     1861 ttcctggatg tgtacatcga acgtgacctg aaagctggca agatcaccga acaagaagcg
     1921 caggaaatgg ttgaccacct ggtcatgaaa ctgcgtatgg ttcgcttcct gcgtactccg
     1981 gaatacgatg aactgttctc tggcgacccg atctgggcaa ccgaatctat cggtggtatg
     2041 ggcctcgacg gtcgtaccct ggttaccaaa aacagcttcc gtttcctgaa caccctgtac
     2101 accatgggtc cgtctccgga accgaacatg accattctgt ggtctgaaaa actgccgctg
     2161 aacttcaaga aattcgccgc taaagtgtcc atcgacacct cttctctgca gtatgagaac
     2221 gatgacctga tgcgtccgga cttcaacaac gatgactacg ctattgcttg ctgcgtaagc
     2281 ccgatgatcg ttggtaaaca aatgcagttc ttcggtgcgc gtgcaaacct ggcgaaaacc
     2341 atgctgtacg caatcaacgg cggcgttgac gaaaaactga aaatgcaggt tggtccgaag
     2401 tctgaaccga tcaaaggcga tgtcctgaac tatgatgaag tgatggagcg catggatcac
     2461 ttcatggact ggctggctaa acagtacatc actgcactga acatcatcca ctacatgcac
     2521 gacaagtaca gctacgaagc ctctctgatg gcgctgcacg accgtgacgt tatccgcacc
     2581 atggcgtgtg gtatcgctgg tctgtccgtt gctgctgact ccctgtctgc aatcaaatat
     2641 gcgaaagtta aaccgattcg tgacgaagac ggtctggcta tcgacttcga aatcgaaggc
     2701 gaatacccgc agtttggtaa caatgatccg cgtgtagatg acctggctgt tgacctggta
     2761 gaacgtttca tgaagaaaat tcagaaactg cacacctacc gtgacgctat cccgactcag
     2821 tctgttctga ccatcacttc taacgttgtg tatggtaaga aaactggtaa caccccagac
     2881 ggtcgtcgtg ctggcgcgcc gttcggaccg ggtgctaacc cgatgcacgg tcgtgaccag
     2941 aaaggtgctg tagcgtctct gacttccgtt gctaaactgc cgtttgctta cgctaaagat
     3001 ggtatctcct acaccttctc tatcgttccg aacgcactgg gtaaagacga cgaagttcgt
     3061 aagaccaacc tggctggtct gatggatggt tacttccacc acgaagcatc catcgaaggt
     3121 ggtcagcacc tgaacgttaa cgtgatgaac cgtgaaatgc tgctcgacgc gatggaaaac
     3181 ccggaaaaat atccgcagct gaccatccgt gtatctggct acgcagtacg tttcaactcg
     3241 ctgactaaag aacagcagca ggacgttatt actcgtacct tcactcaatc tatgtaatta
     3301 gatttgactg aaatcgtaca gtaaaaagca tacaataaag gctccacgaa agtggggcct
     3361 tttttagcac gagagccttt tttgtcagct atctatactt taaggtgact gccaaaacag
     3421 actcgacgta gccttcgagc tgcgcaccaa cacggcctca gatgggccac atctggagaa
     3481 acaccgcaat gtcagttatt ggtcgcattc actcctttga atcctgtgga accgtagacg
     3541 gcccaggtat tcgctttatc acctttttcc agggctgcct gatgcgctgc ctgtattgtc
     3601 ataaccgcga cacctgggat acgcatggcg gtaaagaagt taccgttgaa gatttaatga
     3661 aagaggtggt gacctatcgc cactttatga acgcttccgg cggcggcgtt accgcatccg
     3721 gcggtgaggc aatcctacaa gctgagtttg ttcgtgactg gttccgcgcc tgcaaaaaag
     3781 aaggcattca tacctgtctg gacaccaacg gttttgttcg tcgttacgat ccggtgattg
     3841 atgaactgct ggaagtaacc gacctggtaa tgctcgatct caaacagatg aacgacgaga
     3901 tccaccaaaa tctggttgga gtttccaacc accgcacgct ggagttcgcc aaatatctgg
     3961 cgaacaaaaa tgtgaaggtg tggatccgct acgttgttgt cccaggctgg tctgacgatg
     4021 acgattcagc gcatcgcctc ggtgaattta cccgtgatat gggcaacgtt gagaaaatcg
     4081 aactcctccc ctaccacgag ctgggcaaac acaaatgggt ggcaatgggt gaagagtaca
     4141 aactcgacgg tgttaaacca cctaagaaag agaccatgga acgcgtgaaa ggcattcttg
     4201 agcagtacgg tcataaggta atgttctaat ttccccgtaa agcggcaact ttattgagtt
     4261 gccgcttttt tattccgcgc ttcattctgc tacagcgttt ttaacacatt ttatagtgca
     4321 tctaccatat cccgtacctg agataataac gactgataat agctcgcatg aagttcttgc
     4381 ccatcgaata ccactgtatt acacaagaaa gtgacatcgg catcttcaat accaagataa
     4441 ctgcacatat ttttcaaata atcgcttata tttttttccc agcccatctg gacaaatgat
     4501 tctttgtctc ctccaaccag cgccacccaa cgaactttat tgaatggtaa tttgtgccca
     4561 tctccataag ccagcccatt attccatact ctgtcaatat atccttttag cattgccggg
     4621 aagctatacc accagagagg aaaaaccacc actaacgtgt catgttcaag cagctctgaa
     4681 taaagctgat gaacctctgg agaataacgt ttatccatat tcttccagtc tggttcatct
     4741 tccggcgtca tcactgggtt gaaattacgc cgatataaat cgagttccgt cacctgtatt
     4801 ttccgctcca ttgcccgctg atggatcgca tcagcaatat ttgcggtcaa tgaatcatga
     4861 cgaggatggg cccataccaa ataaatacgt tcagactgca taattttacc tccacgttaa
     4921 gttctggagc gcactttacg caggcggcat aatcctgtaa acgtgatgat tcaggatgag
     4981 ttaatgaagg attttcacta atgcggatga atatgtctga ctttgccact ttctttgccg
     5041 tcgcccgtaa tcaaagcttt cgtgcagcgg gcgatgagtt aggcttatcc tcgtccgcca
     5101 ttagccatag tattaaaaca ctggaacaac gtcttaaaat tcgcttattc aatcggacaa
     5161 cccgaagcgt atcgttaacg gaagcgggtt ctaatctcta tgaacgattg cgcccagctt
     5221 tcgacgaaat tcagatcatg ttagatgaga tgaatgattt tcgtctgaca ccgacaggaa
     5281 cgctcaaaat caatgccgcc cgggtagctg cacgtatatt tttaatgcct ttactggttg
     5341 gtttcacacg tgaatatcct gatattaaag tcgagctcac aactgacgat tctcttgtcg
     5401 atatcgtcca acagggcttt gacgctggcg ttcgacttag tggcatcgtc gaaaaagata
     5461 tgatcagcgt ggcgatcggc ccaccagtga aattatgtgt agcagcaacg ccagagtact
     5521 ttgcaagata tggcaaaccc cgccatcctc atgatctgct taatcatcaa tgtgttgttt
     5581 ttcgttatcc cagcggcaaa cctttccact ggcaatttgc aaaggagctg gaaattgctg
     5641 tcgcaggaaa tatcattctg gatgatgttg atgctgaact ggaggccgtt ttgatgggtg
     5701 ctggtatcgg gtatctgctc tatgaacaga tcaaggagta ccttgatact ggtcgtttag
     5761 aatgtgtact gggagactgg agcacagagc gtccaggctt ccagatctat tatccaaacc
     5821 gccagtatat gtcctgcggc ttgcgcgcat ttctcgatta tgtaaaaacg ggtcagatct
     5881 gccagagtca gcgtcaccga ccacaataat tatttactat tcgctttacg cgccagcagc
     5941 gggaaaatca gtcctaatcc taccagcaca aacggcgttg caacattcag cgccaactgg
     6001 aaggtccact ctgcagtgaa ggcttccatt ttcgggaaga tccccgtcaa acaggcaaag
     6061 gcggtaaagg caaaacacca aatcccgacg gtcattgcca gaggcttatt acgaataaag
     6121 acatattctg gtttatattt ctgcgccagg cgaacgactg caataaatgc aacaaatacc
     6181 cataaataac gcagcggcat cactaccgag ttaagattca acagccattt atagagattg
     6241 ttcatatcac cgatgccgag agtcggcagc ataatcagaa ttgccaccag caccagggtc
     6301 agaaaatagc cattaacggg cgtaccagaa gcgttggtac gacataaact ggctggaata
     6361 tatttgctgt cagcatcacc taatagcact ttaagcgggg catcaatcga gaataccagc
     6421 gcagctactt gtcccagggt attcgcaatg gcgtaaatca ccattaaagt attacccatg
     6481 ttgtaatact cgcccagctt ctgaaaggcg taatattgac cgttagtcat taagtcatcc
     6541 gggatattac gcgagtcaaa catcatcccc atcgccagtg agcccagaat ggcacaaacc
     6601 gcaaccatca ccgccaggca tagcatccct tttggaaatt cttttcctgg gttgcgcgtt
     6661 tgattaacgt aaggagaaat cttctctgct ccgccaaccg cgaaaaccag cattgaaata
     6721 gtggtaatgt aggtaaagtc gatatgagga atgaacgttt cccaggtaat gtttgtggtc
     6781 gcaatatgca cttcagtaat cgcagacgcg gttaccgcca tcgccacata caggagcgac
     6841 ataacaaaca tcgcaatccc tgccacagaa ccgacgattt tcagcgattt cataccgcgt
     6901 gaagcaaccc acataaagaa gataaacagc accagcgtta acccctgtaa cgctacgact
     6961 gaatattctt tgattagcga accgtcgcct ttcatcgccc aaccgagcgc aatcagaatt
     7021 gcctggggtt tttgtgccag aaagggaata tgcaccaccc agtaggtcca cgcggcgaga
     7081 taagccagtc cgggtcccat cgtgtgttta atccaggtac tgaccccgcc cttcccatct
     7141 ttgaaggtcg agcctaactg accaacaatt agcgcataag gtgtgaaata gagtgcaaag
     7201 ataaacaccc atgaaaaaac aaccaccagc ccctggttgg catagttatt aacaacgttg
     7261 ccaaaacccc agacagtgat aaaagacatc agcgcaatgt tgtaccatcg caactgtttt
     7321 tcctgaacat tcccagccat agacaaatcc ttcgccaaaa ttaaaggggc atctgtaaaa
     7381 cgagggatta tgcgcggcaa ataagaagtg cggaaatagc gaaatgcaaa attataacct
     7441 gctgcacatt ttatttttaa acgaataaaa tttggaataa agcaagtcag atagaagaaa
     7501 aaaaaagcag agtgtgaaac agtaagttca cactctgctt gaattcattt acacgtgagc
     7561 aacgggtttc ggcgtatgac cggcgttgcg cagcagcatc agcaaataga taaacgatac
     7621 gctggcgatc atgataaaca ataaattatc ggagaaattc tgcattagca tagcggtaaa
     7681 tgacgggcca agcagacttc ccacagtata gctcaacagt aaggcctggt tcatcgccac
     7741 cagttgatga tgttcaactt tctcgcaagc ccatgccatc gccaccggat atagcgtaaa
     7801 gccagcggca ccgaggatga ataacgctgg ggccatcgcc gcctggctaa gcatcgcgat
     7861 actgccgaga atgacgacaa agacctgaac acgcaacacc agcagtcgac caaacttatc
     7921 cgccagacgt ccaatcggcc attgtccaag gatacccgca ctgaccagta ccgccatcca
     7981 gaaaccaatg ctggcattgc tcaccccttt gtgattgagg tacagcggca tcaggccata
     8041 tagagatccc agaacgattc ctgagataat gcagccattc acgccaagcc gcgcctgacg
     8101 gagttttagc attgacgtaa ttgacgtcga atcatggttt tcagcctgct gattcagcac
     8161 acgcgtaaac aacagcggta agatccctgc caacgtcaaa cctgtaaccc acggcaatac
     8221 ggacatcagc tcggttgaaa ctttgctgac cagtaactgg cctaaaaacg ttcccacgta
     8281 ataaaccatc atatacgcag caagcaaacg cccacggtta cgtgacgtcc cactgcacat
     8341 cagcgcgctc tcaacaacca cccaaatcat ggcacagccg acgcccgcga caaaacgcca
     8401 agccaaccag ctccagaatc caatcatcag gccaaggccg gcacagccag cggcaaaaat
     8461 gaaggaggcc agataatagc tgcggttaaa gccaatgcgc ttaatgacat accctgtcag
     8521 caatgtaccg acaaggttgc cggtaaaata ggatgagctg acaacgccta cctgccatgt
     8581 ggacatgtgt tcctgggcga gccaaagcgg cacgagtgta tttaacaccg cgatcgccag
     8641 agtcaacaaa agcaggccag acagcaaaag catgacaggc cgggtatacg tggacatgaa
     8701 ttaaaaccgt gaggaagttc aaatttcatg cgcatcatgc caccgccaaa aacattgtca
     8761 atgggtgaaa atacgcgcat atcgcagcgg cggtattatc gatttatatt actcattcat
     8821 aacgctaatg ctgaagagat gattaatctc atctgattgt ttttattgat ctaacacaaa
     8881 tttcatacgc ttcgttcagt cgaaaaattt catggtcaca gcaaactcat atttcccgtc
     8941 tatgctttca gagcaatgca tcacgtctga ccgatttcag acgtaccact gactctcgaa
     9001 aggagaagag gatgaccaaa ccgtatgttc gtcttgataa aaatgatgct gccgttttgc
     9061 ttgttgatca tcaggctggt ttactttccc ttgtacggga tatcgaaccc gataagttta
     9121 aaaataacgt gctggcgctg ggtgacctgg ctaagtattt caacttacct actattctta
     9181 ccaccagttt tgaaaccggt cctaacggtc cattagttcc agaactgaaa gcacaattcc
     9241 ccgatgcacc ttacattgct cgcccgggaa atattaacgc ctgggataac gaagattttg
     9301 taaaagcagt caaagcgaca ggtaaaaaac agttaattat tgccggtgtg gtaaccgaag
     9361 tttgcgtggc attcccggcg ctttcagcca ttgaagaagg gtttgatgtc tttgttgtga
     9421 ctgacgcgtc agggaccttt aatgaaatta cccggcattc ggcatgggat cgcatgtcgc
     9481 aagcaggtgc gcaattgatg acatggttcg gcgtggcgtg cgagctgcac cgtgactggc
     9541 gtaatgatat tgaaggactg gcgacgttgt tctctaacca tattcctgat tatcgtaatt
     9601 tgatgaccag ttatgacacg ttaacgaagc agaaataaaa gagaaccggg gccgaaaccc
     9661 cggttgttac tgattagctt gcgacggcca tccccacggt catatgcaaa ccatagaata
     9721 cgccgcgacc gattaattcc cctgccagaa gcagaatgaa tgagacggat agtagcggaa
     9781 ccgcaggctg ataacctttt agctgtggtg caatccacaa gcacagggca acggccaaaa
     9841 gcacgatccg ccaggacatc agcgcaccat agtccggcac cagcgctgcg gcctgctgca
     9901 cagaactatg aatagttgcc agctctgtgc cctgcatcac tgacatcacg ccactcacca
     9961 ccagtgccag tacagaaatg gctggcagca gacgcatcgc ccagccatcg acgccagcca
    10021 gactcaacaa caggtaaccc agtaacgggc cgcccataaa catcgtcagg aagaagccca
    10081 tcggcgtcca gatgctgtac caggtcggaa cggtatcaat gctgttatac acacgcacca
    10141 tcatccagac aaagatgacg ccaagaacca tcgtcactat cagccacagc gtacgcaatg
    10201 ccggtgacag ctttttcagc attgccagca accagccgat gccgcctacg gcaaaaaaga
    10261 tcgaaccgct ggcgatttcg ttactgagtg ctgaagcccc tacccggttg agcgagttaa
    10321 aagcgcgcat tggtgaacca agatggagca tagaggcgat aaagccaatg cccatcagca
    10381 cccataaacc aaacatgcag gcgataacac gctgctgggc ttctgctcgc aggtcgcctt
    10441 tgagcagcgc caaagccaga acgataaaac cacctgctac acattgcccg aagaccgtga
    10501 agatcatcag cggccattca tgccatccac ttcccatctc acacctcctt cgggtttgcc
    10561 agatagccag tggtatcccc ggtcgggcgg ctattggcat tgggtttgat cacaatattc
    10621 ggtttggtaa agtgagctcg cggcaacggc gcaacggccg ccagatcgcc atgtttttta
    10681 cgcagctcgt cgatagggcc gaaatccagc gcccgcagcg gacaagattc aacacagatc
    10741 ggctttttac cctccgcaac acggtcataa caaccatcgc atttggtcat atggcctttc
    10801 gtttcgttat attgcggtgc gccatacggg caagccatat ggcagtagcg gcagccaatg
    10861 cacacatctt catcgaccac aacaaaacca tcttcacgtt tatgcatcgc accgctcggg
    10921 cagactttag tacaagccgg atcttcgcag tggttacatg aaatcgacag atagtaggca
    10981 aacacgttct ggtgccagac accgttatct tcctgccagt cgccgccagc atattcataa
    11041 atgcggcgga agctgacttc tggcgtcaaa tctttgtagt ctttacaggc cagctcgcag
    11101 gttttgcaac cggtgcaacg gctggaatca ataaaaaatc catactgggt tgtcatcggt
    11161 tactccttac accttttcaa cctgaacaag gtttgtatgt gacggattcc ccttagcgag
    11221 aggagacgga cgttgagtgg tcagtacgtt gatacaaccg cccttatcga cacgttttgt
    11281 atccgggtca taccaggcac cttcacccag tgcgaccaca cccggcatca ttcgtggcgt
    11341 cactttcgcc tcaatatgaa cctcaccacg atcgttaaag atcctgactt tatcgccgtt
    11401 gttgataccg cgtttttgcg catcaagcgg gttgatccac atttcctgac ggcaagctgc
    11461 tttcagcaca tcaacgttgc cgtaagttga gtgaacgcga gatttatagt ggaaacccgt
    11521 aagctgcagc ggatactgtt tgttcagcgg atcctgataa ctttcaaagc ccggcgtgta
    11581 gatcggcagt ggatcgatca catcgccttc tggcaattcc caggtagcgg caatgtcagc
    11641 cagcgcctgc gaataaatct caattttgcc cgatggcgta gtcagtgggt ttgcctgcgg
    11701 atcttcacgg aaggctttat aagcaacgtg atgcccttgt gggtcgcgct ttttaaagat
    11761 cccctgcttg cggaactctt caaacgttgg cagttcagga atcgcttccc gcgactgggc
    11821 atacagatgc cgcatccact cttcctgggt acggccttca gtaaactgtt gctcaacgcc
    11881 aagacgtttt gccagttcgc tggtcatttc atagatggtc ttacattcaa agcgcggttt
    11941 aatcacctga tcgttgaaaa tcacgtaaga catattcccg caggatgcat ccagtgcaaa
    12001 gtccatctgt tcggaagcgg tgcagtcagg cagcaggatg tcagcatatt tcgccgatga
    12061 ggtcatgtgg cagtcgataa ccacaatcag ctcgcacttc ttatcatcct gaaggatttc
    12121 atgggtacgg ttgatttcag aatgctggtt aatcaggcag ttaccggcat agttccagat
    12181 cattttgatc ggcacatcca gcttatcttt cccgcgtaca ccatcacgca gcgccgtcat
    12241 ttccgggcca cgttcaatgg catcggtcca cataaacatc gaaatgctgg tctggatcgg
    12301 gttttccaag gtcggcatac ggacaaacgg taagctgtat gaaccttcac gcgcgccgct
    12361 gttgcctccg ttaataccaa cgttaccggt cagaatcgcc agcatcgaga tagcacgggt
    12421 tgcgatttca ccgttagcgt gacgctgcgg gccccatccc tggctgataa acgccggttt
    12481 ggtactgccg atttcacgag ccagtttgat gattttgtct gccggaacac cggtgatttg
    12541 tgaggcccat tccggtgttt tagccacgcc atctggccct tcacccagaa tataagcttt
    12601 atagtggcca tttttcggcg cactggctgg cagggttttc tcatcgtagc caacgcaata
    12661 tttatcgagg aatgcctgat ccaccaggtt ttcagtgatc atgacgtacg ccagaccgtt
    12721 aaccagtgcg gcatctgttc ccggacgaat agggatccac tcatcttcgc gcccggcacc
    12781 ggtgtcggta tagcgcggat caatgatgat catgcgggca ttagatttct ggcgtgcctg
    12841 ttcaagatag taagtcaccc caccgccact cattcgcgtt tcgccagggt tattaccaaa
    12901 cagcactacc agcttactgt tttcgatatc cgacgggctg ttgccatctg cccagccgcc
    12961 gtaggtatag ttcaaacctt ccgcaatttg cgcggaggag tagtcgccgt aatggttcag
    13021 atagccgccg cagcagttca tcagccgcgc gaccagggta tttcccggcg gccaggagcg
    13081 ggtcatggtg ccgcccagcg taccggtgcc atagttcaga tagatagact cgttgccgta
    13141 ctctttgatc aggcgctgca tattggtcgc gatgatgtcg taggcttctt cccagctaat
    13201 gcgctcgaat ttgccttcac cgcgcgcccc gactcgtttc atcggatatt tcaggcggtc
    13261 cggattgtag acacggcgac gcatggaacg cccacgcagg caggcgcgaa cctggtgcag
    13321 gccgtcgtaa ttgtcatcgc cggtattgtc cgtttcgaca tatttgattt caccgtccac
    13381 gacgtgcata cgtagcgggc agcgactacc acagttaact gtacaggcgc tccagataac
    13441 cttttcgtct gattttgttg gaatggcgct atcgacagcg tgcgcaatcc gactaaaagg
    13501 taatgttaat gcgctgctgg ccattgccag gccgccgatc gctgtcgttt ttaccaaacc
    13561 acggcgactc acctcagcag ccaataccgc atcagggatt ttcgttttca taatggctca
    13621 ctcaagcttg ctcacgattg attccagtaa caaaccgtat tgtgtaatta tttctatagc
    13681 agctcgctat ataggcttgt atacatcgaa taatttgaaa atcaaagaga taggtagatt
    13741 ttcactcgaa agtagtatta cgtgtaagtg aggagtaatt attgttcggt atcaaaggga
    13801 gacaaaaaaa gcgcccgcag gcgcttttta gattcagaaa aattgggtat tagccaatat
    13861 attccagtcc gttcatatac ggacgcagaa cttctggtac ttcaatacga ccatcagcct
    13921 gctgatagtt ttccattact gcaaccagcg tacgaccaac agccagtcca gaaccgttca
    13981 gggtatgaac cagacgggtt ttcttgtccg acttgctgcg gcagcgtgcc tgcatacgac
    14041 gcgcctggaa atcccaaacg ttggagcagg aagagatctc acggtaggtg ttctgtgccg
    14101 ggatccatac ttccaggtcg taagttttgc aagcgccaaa gcccatgtcg ccagtgcaaa
    14161 ggatgatttt acggtacggc aggcccaaca actgcaggac tttttctgca tgaccagtca
    14221 tctcttccag cgccgccatt gagtcttccg ggcgcacgat ctgcaccatt tcaactttgt
    14281 cgaactggtg catacggatc agaccacggg tgtcacgacc atatgaaccg gcttcagaac
    14341 ggaagcatgg ggtgtgggcg gtcatcttaa ttggcagatc atcttcatcg atgatttcac
    14401 cgcgcaccag gttagtcagc ggaacttctg ccgttgggat cagcgcatag ttactggtgt
    14461 ctgcttcttc ttccagcgga cgagtatgaa acagatcgcc agcaaatttc ggcagctgac
    14521 ccgtaccgta cagcgtgtcc tggttaacca ggtacggaac atagttctca ctgtagccat
    14581 gctgttcggt atgcagatcc agcataaact gcgacagtgc gcggtgcatg cgagcaatct
    14641 gccctttcat taccacaaag cgggaaccag tcagcttaac tgcagctgca aagtcgaggc
    14701 cagagtgcat ttcacccagc gtcacatggt cacgaacttc aaagtcaaac tcacgcgggg
    14761 tgccccagcg gctgacttcg acgttgtcat tttcgtcttt acctaccggc acttcatctg
    14821 caggcaggtt agggatggtc agcgcgatat cgcgaatttc agcctgtaaa gcatccagct
    14881 cggcttttgc tgcatccagc tcttcgccca gtttgttcac ttccagacgt aaaggctcga
    14941 tatcttcccc gcgcgctttc gcctggccaa tggatttcga tcgggagtta cgctccgctt
    15001 gcaggttttc cgttttgacc tgcaatactt tacgacgctc ttcaagagcg cccagcttat
    15061 ctacatccag cttaaagccc cggcgtgcca gtttttcagc gactgcgtct ggctcattac
    15121 gcagcagatt gggatcgagc atgcttatcc tgtgcttatc gaattaaatg ggagatgtgg
    15181 ccacagcctg cgaccacagg gatacagtaa caacattacc gcaacgataa cattaacggt
    15241 agcgttttat ggggctattt tgatcctgtt cagccagcca ggcgagcttt tcgccaatct
    15301 tgccttcaag gcccctgttt gtcgggaaat aatagcgtgt ttgtgctatt tccggcggga
    15361 agtaaacctc accggcagca taagcgtttg cttcatcatg agcgtaacga tattcctgcc
    15421 cgtagcccat ttccttcatt aatttcgtcg gcgcattacg caaatgaacc ggcacgtcat
    15481 aatccggacg ttcgcgagca tccgccagcg cagctttaaa cgcagtgtag acagcgttgc
    15541 tttttggcgc gcaggccagg taaacaatcg cctgagcaat ggcgcgttca ccttccgccg
    15601 ggccaacgcg agtaaagcaa tcccaggccg caattgccac ctgcatcgcc cgtgggtcgg
    15661 cattgccgac atcttcggaa gcaattgcca ggcaacgacg cgcgacatat aacggatcgc
    15721 caccagcggt aataattcgc gcataccagt agagcgccgc atcgggtgcg ctaccacgta
    15781 ccgacttatg cagtgcggaa atgagatcgt aaaagcgatc gcctttgtta tcaaagcggg
    15841 cgcttcgctc accggcgatt tcggtcaata attcaggctt caggacccgc ttaccgctat
    15901 cattgacttc ggccatatcc gccatcattt ccagcgtatt taacgcccgg cgcgcgtcgc
    15961 cattcaccag ttcagcaatg gcgcgtcgtg tttcatctgg cagaacaata tcctgaccac
    16021 cataaccacg ggtcttgtct tccatcgcct gagttagtac ttgctcaata tcctctgtac
    16081 ccagggattt caacagatag acacgggcac gggaaagcag tgccgaatta agctcaaacg
    16141 acgggttttc agtggttgcg ccaataaaag tgatggtgcc gtcttcaata tgtggcagaa
    16201 atgcatcctg ctggcttttg ttgaatcggt gcacttcgtc aacaaaaaga atagtgcggc
    16261 gacctgcatt gcggttttgc cgggcgcgct cgatcgcctc gcgaatctct ttcacgccag
    16321 aggtgacggc agaaatacgt tccacatcag cgttcgcata gcgggcaatc acttcagcga
    16381 gggttgtttt gccggtaccc ggtggccccc agaggatcat agaatgtaaa tgcccggctt
    16441 cgatagcgcg cggcaacggc ttccccgcag ccagcaaatg ttgctggccg atatactgtg
    16501 ctaaattttc tggccgcata cgcgcggcca gaggttgaaa agtattatcc gaaaaatcga
    16561 gcgacagatt gctcactcag gtgcctctac ttacgttgat catctaccgt gacgccttgc
    16621 ggcggggtga aggtaaattt cgctgcatcc acagccccat tttgctggga tttcagttga
    16681 taactgctgc gctgatcgtc ctgctccacc gcgctaaact gatggattgt gccatcacgt
    16741 cccacgttaa tggtgaactg cttcagattg ccattgctgg ctttcggcgt caggacaaag
    16801 tcatcgccat tctgtttgat attgtactgc tgccagtcgc tggactggtt gcgggcaatc
    16861 agcataaacg gcgtattacc ggtggcatct ttcagccagg ttgccgtagc ttgctcaacg
    16921 aacgggttat agaaccacag tgttttaccg tcagaaacca gaatgctttc atcaggttgt
    16981 gtcatatgcc agttgaataa gtttggacgt ttcacccaca gatcgccctg accttcctgc
    17041 accgccgcgc cgctaccgtc agtcactttt tgtgtgaagc tggcgtggaa gctgctgact
    17101 ttatccaggc ggcttttcag atcgcttgcg gcatcagccc aaacgctgct tgctactaag
    17161 cttgagagta atgcacaggt gatggcaatt tttttcatca ttattcctca aattacgtca
    17221 ctcccgatcg ggagttccct ctactctcta ttccaggcta ataatcagct tgaggaaaga
    17281 agaaaatact gaattttcag ttgatagagt gccggatgcg acgctaccgc gccttatccg
    17341 gcaatgcatt aattagtcaa acggcggtgg ggccagcact tcacgattac cgttgtgccc
    17401 ctgttcgctg acaatcccct gcgcttccat ctgttcgata atacgcgctg cacggttata
    17461 accaatgcgg aactgacgct gtacgccaga aattgacgct ttgcgttttt cagtgacaaa
    17521 ctgcaccgcc tgatcgaaca acggatccag ttcttcagcg ccatcgaaac cgcccgcgcc
    17581 accttcgctt tcactgtcgg aagtgatgcc atcaacatac tgtgggcgac cacgcgcttt
    17641 ccagtcctgc accacggcat gaacttcctg atcgcgaaca aaagcaccat ggacacgtac
    17701 cggcaacgtg gagttcggcc cagagtagag catatccccc atccccagca gtgattccgc
    17761 gccagcctga tcaagaatgg tacgtgagtc aatcttactg gatacggtaa aggcgatacg
    17821 ggtcggaata ttcgctttaa tcagaccagt aataacatca accgatggac gctgagttgc
    17881 cagtacgagg tggatacccg cggcacgggc tttttgcgcc agacgtgcta tcagctcttc
    17941 cactttttta cctaccgtca tcatcaggtc ggcaaattcg tcaaccaaca ccacaatgta
    18001 tggttctttt ttcagcaccg gatgctgggc atccatactg tcacccggct tccagtacgg
    18061 gtctggaatc ggacgcatca tgcgatcggc ttcagcaatt ttttcgttat aacccgccag
    18121 attacgcaca cccagcgcag acatcagttt ataccgacgc tccatctcgt taacacacca
    18181 gcgcagcgcg ttggcggcat ctttcatatc agtaacgact tccgttaaca gatgcggaat
    18241 gccttcataa accgaaagct ccagcatttt cgggtcgatc atgatgaaac gcacatcttc
    18301 tggctgtgct ttataaagca tgctcaggat catcgcgttc acaccgacag atttaccgga
    18361 accggtagtc cccgcaacca acaagtgcgg cattttcgcc agatcggcaa ccaccggctc
    18421 accggcgata tctttaccca gcaccacggt taatggcgac ggattatcgc ggaatttggc
    18481 gttatccaaa acttcgcgca gataaacggt ttgtcgtttt ttatttggta actccagacc
    18541 tacatagggt ttgccaggaa taacttcaac gacacgcacc gccaccgtcg aaagtgaacg
    18601 ggcaaggtcc cgtgacaagt tagaaatgcg cgccgctttt acgccaggag ccaggttaag
    18661 ttcaaagcga gtgataaccg gccccggaga gtaattgacg acatcggctt taatacggaa
    18721 atcagccaga cgcgcttcca ccaggcgagc catttgttca agcgcaaagg tatctaccgg
    18781 ctccacttcg ctcggcggcg gtgtcagcaa atccagagaa ggcagcggcg tcgtcggttt
    18841 atgcaacgga cggctgtcgc cattacgcat caacagcgga tgaagcaggg tatcctgctg
    18901 ctgcggtgca accggttgtt gcggctgttg atactgcggc tgcggagcaa ctggttgttg
    18961 cggctgctga tattgcggct gtggcgcaac cggttgttgc ggctgctgat attgtggctg
    19021 cggcgcaacc tgctgttgtg gctgctgata ctgcggctgc ggcgcaaccg gctgttgcgg
    19081 ctgctgatat tgctgctgtg gcggaactgg ttgttgcggc tgctgatact gctgctgcgg
    19141 tgcaaccggt tgttgcggct gctgtacagg ttcaacaatt ggcgtaaaca acggttcgtg
    19201 tggaccatca tccagcaacg ctttcattgg cgaaaattca aaatcatcca gcgagaacgg
    19261 attcgcccca gccggttgtt cgccggaata acgttgttgt tgagtttgcg caaactgacg
    19321 agccagttca gcctctgccg cagcatctgc atcttctgcg tttacgggca catcatgttg
    19381 atactgttcg ccatagcgtt gctgctgtgt ctgggcgaac tgacgtgcca gttcatcctg
    19441 ctgcatcgca tcgatttcat catcgttata ctgatcgcca gaatcatact gattgcgctg
    19501 ggcttcacgg gctttctctt ccgccgcacg ctgtgagggc agcttaatac cgtaagacgc
    19561 cagttcacga cgagttggca cgcggatacg tttcggtcgt ggcaactgcg ggccaatccc
    19621 ctctttgact tgcggacgcg gtccaccgct atttgccaga ctgaagactg gcgcggcaac
    19681 ggttgcggca gcccccgtcg ccagtgtcgc ttttttcacg ccagatgcca gcggagaaac
    19741 agcggcagcg gcttctactg gaggtactgc tgcaacagaa ggcgctttca acgaagattt
    19801 gatcggttct ggttctttaa ccggttctgg aatcggttga taccaggccg caagttgttc
    19861 acgttcacgg gctcgcttct cttccacttc ttcaaaatag taaagcggcg gacgcgcggg
    19921 ttttgtctct tctacaacgg gttcaggctc cacaacaggc tgctgttcaa cggattgcgg
    19981 ctgttggtac aacggctcct gagcggctgg ttgctgataa gtttgctcag tctggtatgt
    20041 agactgtgga gcaaaagtgg attgctgctc ttcggcttgc caggcgttac ctgccaccgg
    20101 ttgttctggc gcaggggcgt aatacggctg ttgtgcaggc tgttccggcg cagttgcgta
    20161 atacggttgc tgaacaggtt gttccgccgc aggagcgtag taaggttgtt gcgcaggttg
    20221 ttcagctgca ggtgcataat acgactgctg cggctgtact ggttgttgca gcggctcatt
    20281 atattgcact gcaggctgcg catattgtga ctgctgtggg taaccttccg gtgcaggagc
    20341 aataaccggc tctcccgttt gtggacccgg tacaggctgc caggctactg taggttgcga
    20401 gggtggaaca tcaacagagg caacaggcgg cgtctgagtc acaggttcaa ccggcgcagc
    20461 ccagctttgt gtcgccgtgg tagcagcagc tgctacagcg acaggttcgg taattggcgc
    20521 accgtttaat aatggatcgt attcgtcata ttctggctgc gttgcacgat tgcccgaaaa
    20581 taggacgtcg tccgggtcgg cagccacacc gcgtgcagtg taggtaatct cttcgtcatc
    20641 atccatccgc ttaccggaga acaacgcagc gtctgtttgc cgccccatcg gattaatgaa
    20701 tttttccgcc aaccgtttac gacgcgctag cgcgccgcga agaatacggg cacggcgtga
    20761 ttcatgctgt ttgccgtggt tttcatcttc atactcttcg tcatcttcat attcatcttc
    20821 atcgacccag gtatcatcgc gacgggtacg attactggcg aaggtgagaa tgtttaaaat
    20881 ccagccgccg agtttttcag caatggtcac ccatgaccaa ccggtgaaca gcgtcaggcc
    20941 cgcagcccaa acgcagagca gcgcaatagt tcccccgcta ctgtgtagca gtggttgtag
    21001 cgtagtactt agtaagctgc caatgacgcc accggaggca aaataccaga tatcgtcagc
    21061 gttgattgcc gccagaccac aggaggtaag gatgagcgcc aaaacgccaa tgatgcgtag
    21121 cgaaacggca aaataatcaa tgtattcgtc gctggactga tgacgccagg caaaccaaca
    21181 accgccgaca ataatgacgg gaatggtgta agccatcacg ccaaaaataa agaacagcgt
    21241 atctgccaac cacgcaccgg gcatcccacc taaattatgg ataggttcat gccaggccgt
    21301 ttgcgaccag ctggggtccg aagggttaaa gcttagtaag gcagccatca accagacggc
    21361 aaacaggaca ataaggatca gcaacgcctc cagaaggcgg cggccgctac ttaactttgt
    21421 caatgtgact tctttgtctt cagtgtattc ctggctcaag aaaggctctc caggttacag
    21481 gcttttcctg cccgatgcta aaacggacaa cagtaccagg ttaccccagt actgttgctg
    21541 tatggattaa caggagtgta atcaaaatac gccgattttg cacctgttcc gtgttagcgc
    21601 gtcttaataa ccagacgatt actctgcttg acttcttcca taacaacgta tgtccgtgtg
    21661 tcattgacgc caggcagacg cagcagggtt tcccccagca acttacggta agctgacata
    21721 tccggcacgc gtgttttcaa caggtagtcg aaatcaccag atactaaatg acactcctga
    21781 atttcttcaa gtttttgtac agcggtattg aattgttcaa acacatccgg tgcgccacga
    21841 ttcagagtaa tctcaacgaa taccagaagt gatgcatcca gataatgggg gttcagcagc
    21901 gccgtatagc cctgaataaa cccttgtctt tccagccgac gcacacgctc aaggcacggc
    21961 gttggggaaa gtcccacacg tttagaaagc tcgacgttag aaatacgccc atccttttgc
    22021 aactcattaa gaatgttacg atcgatacgg tcgagatctt tgccagggcg cttcttgcta
    22081 tctaccatta ttattgtctc tctgtattcc ttccctactc ctgtctgacc ctgaaacatc
    22141 actgttcaga gtcgctaccc gtcacgatag accgaaatca tgaagattca cggcgcgcaa
    22201 tgcctgggtc tacggtgaga agaccgttgc cttgtggcag ctatcgacat cacgaatggc
    22261 gtctgtccac gccatacgta gatttcacta gcacggtaaa catggtattt acatgcatgg
    22321 ttatgcgcat ataagaatac tgtttttttc ttcctcgaat gttttcgcaa aacaccaggg
    22381 gattgtcaaa gcaaaacatc gatttttagt acaacatgcc agatattcat taacaggggc
    22441 gtttattcat catttaatcg gcttataaag cgggaaattt aagtagcaga ggtgataatg
    22501 acgggagaat ttgacctgct tatcgatgct gccgattgca caaattgtta acaaaatcgt
    22561 catactcttt ttttacgtct gtaaattccc tacaatcctg cccattgtct gccaacaact
    22621 atggggatct catgggcacg accaaacaca gtaaactgct tatcctgggt tcaggcccgg
    22681 cgggatacac cgctgctgtc tacgcggcgc gcgccaacct gcaacctgtg ctgattaccg
    22741 gcatggaaaa aggcggccaa ctgaccacca ccacggaagt ggaaaactgg cctggcgatc
    22801 caaacgatct gaccggtccg ttattaatgg agcgcatgca cgaacatgcc accaagtttg
    22861 aaactgagat catttttgat catatcaaca aggtggatct gcaaaaccgt ccgttccgtc
    22921 tgaatggcga taacggcgaa tacacttgcg acgcgctgat tattgccacc ggagcttctg
    22981 cacgctatct cggcctgccc tctgaagaag cctttaaagg cagtggggtt tctgcttgtg
    23041 caacctgcga cggtttcttc tatcgcaacc agaaagttgc ggtcatcggc ggcggcaata
    23101 ccgcggttga agaggcgctg tatctgtcta acatcgcttc ggaagtgcat cttattcacc
    23161 gccgtgacgg tttccgcgcg gaaaaaatcc ttattaagcg tctgatggat aaagtggaga
    23221 acggcaacat cattctgcac accaaccgta cgctggaaga agtgaccggc gatcaaatgg
    23281 gtgtcactgg cgttcgtctg cgcgatacgc aaaacagcga taacatcgag tcactcgacg
    23341 ttgctggtct gtttgttgct atcggtcaca gcccgaatac tgccattttc gaagggcagc
    23401 tggaactgga aaacggctac atcaaagtgc agtcgggtat tcatggtaat gccacccaga
    23461 ccagcattcc tggcgtcttt gccgcgggtg acgtgatgga tcatatttat cgtcaggcca
    23521 ttacttcggc cggtacaggc tgcatggcag cacttgatgc ggaacgctac ctcgatggtt
    23581 tagctgacgc aaaataattt tacaaatcag taacaaaagt aaagaaggcg acaccatgcg
    23641 actatgggtc gcctttattt tttccccgtt gtaacattgc tctgcaaata attctgataa
    23701 ctcacctgct aagcgtgcaa tgaataaatc ccgtcaaaaa gagttaaccc gctggttaaa
    23761 acagcaaagc gtcatctccc aacgttggct gaatatttct cgtctgctgg gctttgtaag
    23821 cggcatattg atcattgccc aggcctggtt catggcgcga attctgcaac atatgattat
    23881 ggagaatatt ccccgtgaag ccctgctgct tccctttacg ttactggttc tgacctttgt
    23941 actgcgcgca tgggtggtct ggttacgcga acgggtgggt tatcacgccg ggcagcatat
    24001 ccgctttgcc atccgccgtc aggttctcga ccgtctgcaa caagcagggc cagcgtggat
    24061 tcagggtaaa cctgcgggga gctgggcgac gttggtactc gagcaaattg acgatatgca
    24121 tgattactat gcacgctacc tgccgcaaat ggcgctggca gtgtcggtgc cgttgctgat
    24181 tgtggtggcg atcttcccct ctaactgggc tgcggcgctc attctgctgg gcacagcacc
    24241 gttaattccg ttgtttatgg cgctggttgg gatgggggct gccgatgcta accggcgtaa
    24301 ctttctcgct cttgctcgct taagtgggca tttcctcgat cgcctgcgcg gcatggaaac
    24361 attgcgtatt tttggtcgtg gtgaagctga aattgaaagt attcgttctg cttcggaaga
    24421 tttccgccaa cggacaatgg aagtgctacg gctggcgttt ttatcctccg gcattctcga
    24481 attttttacc tcgctgtcaa ttgctctggt ggcggtctac tttggttttt cctatctcgg
    24541 cgagctggat tttggtcact acgataccgg tgtgacgctg gctgcgggtt ttctggccct
    24601 gatccttgcg ccagagtttt tccagccatt gcgcgatctc ggtacgtttt atcatgctaa
    24661 agcccaggct gttggtgcag ctgacagtct gaaaacgttt atggaaaccc cgctcgccca
    24721 tccgcagcgt ggcgaggtgg aattagcatc gaccgatccg gtgaccattg aggccgagga
    24781 tctgtttatc acgtcgccgg aaggtaaaac gctggccgga ccgctgaact ttactttgcc
    24841 agcaggccaa cgtgcagtgt tggttggtcg cagcggttca ggtaaaagct cactgctgaa
    24901 cgcgctttct ggttttctct catatcaggg atcattacga atcaacggga tagaattacg
    24961 cgatttatca ccagaatcat ggcgtaaaca tctctcctgg gttgggcaaa acccacaatt
    25021 accggcagca acattgcggg ataacgtact actggcgcga cctgatgcca gcgaacaaga
    25081 attacaagca gcgctggata acgcctgggt cagcgagttt ctaccgctcc tgccacaagg
    25141 cgttgataca cctgttggcg accaggctgc ccgcctttcc gtggggcagg cgcagcgcgt
    25201 ggcggtggcc cgtgcgttac taaatccctg ttcgctatta ctgttggatg aacccgctgc
    25261 cagccttgat gctcacagtg aacagcgcgt aatggaggcg ctgaatgccg cctctctgcg
    25321 ccagacaacg ttaatggtca cccaccagtt agaagatctt gctgactggg atgtcatttg
    25381 ggttatgcag gatggccgga ttattgagca aggacgttac gcggaattaa gtgtggctgg
    25441 tggcccattc gccacattac tggcccatcg tcaggaggag atttaaatgc gcgctttgct
    25501 accctatctg gcactgtata aacgtcataa atggatgtta agtcttggta tcgtgctggc
    25561 aattgtcacg ctgctcgcca gtatcggtct gttgacactt tccggctggt tcctctcggc
    25621 ctcagcggtt gcgggggttg ccggactgta cagcttcaac tatatgctac ccgctgcggg
    25681 cgtgcgtggc gcagcaatca cccgtactgc cgggcgttat tttgaacgtc tggtaagtca
    25741 cgacgcgact ttccgcgtgt tgcagcatct gcgcatttac accttcagca aattgctgcc
    25801 cctctcccct gccggactgg cgcgctatcg tcagggcgaa ttgctcaatc gcgtggtggc
    25861 ggatgttgat acgctcgatc atctttatct gcgcgttatc tcgccgctgg tgggcgcttt
    25921 tgtggtgatt atggtggtga caatcgggtt aagtttcctt gatttcaccc tcgcctttag
    25981 gctgggcggc attatgttac tgacgctttt cctgatgcca ccgctgtttt atcgtgcggg
    26041 aaaaagcacc gggcaaaatc tgactcatct tcgcggacag tatcgccaac aactgacggc
    26101 ctggctgcaa gggcaagctg agctgaccat ttttggcgcc agcgatcgtt atcgcacgca
    26161 actagagaat acagaaattc aatggctgga agcgcaacgc cgtcaatctg aactgaccgc
    26221 attgtcgcaa gcgataatgc tgctcattgg cgcgttagcg gtgatcctga tgctgtggat
    26281 ggcgtctggc ggcgttggcg gcaatgctca acccggcgcg ttaattgccc tgtttgtctt
    26341 ctgcgcgtta gccgcgtttg aagcactggc accagtaacg ggtgcatttc agcatctggg
    26401 gcaagtcatt gcctctgccg ttcggatcac tgatttaacg gatcaaaaac cggaagtcac
    26461 cttccctgat acccaaactc gtgttgccga tcgcgtttcg ctgacattac gggatgttca
    26521 gttcacttat ccggagcaat cccaacaggc acttaaaggg atttctcttc aggtaaacgc
    26581 cggggaacat attgcgattc tcgggcgaac cggatgcggc aaatcaacac tgttacaact
    26641 gctgacccgc gcttgggacc cgcaacaggg agagattttg cttaacgata gccccatagc
    26701 cagcctgaat gaagcggctc tacgacagac catcagcgtt gttcctcagc gagtgcatct
    26761 gtttagcgcc acgctgcgtg ataatctgtt actcgcctcg cctggcagta gtgatgaggc
    26821 tctggcggag agcttgcgtc gcgttggtct ggagaagctg ctcgaggatg caggtctcaa
    26881 cagttggtta ggtgaaggcg gacgccagct ctccggtggt gaactgcgcc gtctggctat
    26941 cgcccgtgcg ctgttacatg atgcgccact ggtgttgctg gatgaaccta ccgaaggctt
    27001 agatgccaca accgaaagcc agatccttga attgcttgca gaaatgatgc gtgagaaaac
    27061 ggtgttaatg gtcacccatc gacttcgcgg actctctcgt ttccaacaaa taatagtgat
    27121 ggacaacggg caaattattg agcaaggtac tcacgcagag ctgcttgcca gacaggggcg
    27181 ttattaccag ttcaagcagg gtttgtaagc tattattgaa cgatccgact tgcgtggagt
    27241 tttgcggtca tgcgcctggt tcagctttct cgccattcaa tagccttccc ttccccggaa
    27301 ggcgcattac gtgagcctaa cggcctgctg gcacttgggg gcgatcttag ccctgcgcgc
    27361 ctgttaatgg cttaccagcg tggtattttt ccgtggtttt ctccaggcga ccccatcctc
    27421 tggtggtcgc ccgatccccg cgcggtgcta tggccagaat cactgcatat cagccgtagt
    27481 atgaagcgat ttcataaacg ctcgccctat cgtgtcacga tgaattacgc ttttggtcag
    27541 gtcattgaag gctgtgccag cgatcgcgaa gaaggaacct ggatcacgcg tggcgtggtc
    27601 gaagcctacc atcgccttca cgaactcggc catgcccact ccattgaagt ctggcgtgaa
    27661 gatgagcttg tcggcggtat gtacggcgtg gcccagggaa cgctattttg tggcgagtcc
    27721 atgttcagcc ggatggaaaa tgcgtctaaa acggcgcttc tggtattctg tgaggaattt
    27781 atcggtcatg gcggtaagct tatcgactgc caggtcctta acgatcacac agcatcgctt
    27841 ggtgcctgcg aaattccccg ccgggattac cttaattatc tcaatcaaat gcgcctcgga
    27901 cgattgccga ataatttctg ggtaccacga tgcttgtttt caccacaaga atgaatgttt
    27961 tcggcacatt tctccccaga gtgttataat tgcggtcgca gagttggtta cgctcattac
    28021 cccgctgccg ataaggattt tttcgcgtca ggtaacgccc atcgtttatc tcaccgctcc
    28081 cttatacgtt gcgcttttgg tgcggcttag ccgtgtgttt tcggagtaat gtgccgaacc
    28141 tgtttgttgc gatttagcgc gcaaatcttt acttatttac agaacttcgg cattatcttg
    28201 ccggttcaaa ttacggtagt gataccccag aggattagat ggccaaagaa gacaatattg
    28261 aaatgcaagg taccgttctt gaaacgttgc ctaataccat gttccgcgta gagttagaaa
    28321 acggtcacgt ggttactgca cacatctccg gtaaaatgcg caaaaactac atccgcatcc
    28381 tgacgggcga caaagtgact gttgaactga ccccgtacga cctgagcaaa ggccgcattg
    28441 tcttccgtag tcgctgattg ttttaccgcc tgatgggcga agagaaagaa cgagtaaaag
    28501 gtcggtttaa ccggcctttt tattttgtga tatgtatgaa gtactttgga agtataagtc
    28561 cgtaacttgt ctcgatgtag atatactgca caacttgttt acagtatgag caaacaataa
    28621 ttatcattaa aattcttctt gaccgtttta ctccaactcc ctatagtagc gccccgttgc
    28681 cacccatgag gtttggtagc aaaacaattt ggtgaggtgt ccgagtggct gaaggagcac
    28741 gcctggaaag tgtgtatacg gcaacgtatc gggggttcga atccccccct caccgccata
    28801 tttaaagaag agctcgtacg aaagtgcgag cttttttttc gcattttatc tgtctgtacg
    28861 gcagtgaacg cgttcgcaaa ggttttaccg caccccgtcg atttctaagc tgcctgtacg
    28921 gcagtgaact caaatactac acgtttagcc cgttgttaat aaagcaaaac catcccattc
    28981 tcctgcagca ccctttttta tccttacctt cccgcagcac taattatcaa caacttacca
    29041 tcatcattaa aaaagggtct gaacctcctc caccccataa acaaaaagtc cgtaacctct
    29101 ttcgagatta cggacttgac caacctacct aacaatcaga ttaatgcgct gcttccgcct
    29161 tgtgcttttg tgcactctgg aatccgtaag tcagctcatt tttctcttta tccagcgcga
    29221 cggtgacctg accgccgtcc accagcgaac caaacagcag ttcgttggcg agcggttttt
    29281 tcaggttgtc ctggatgaca cgcgccatcg gacgtgcgcc cattgcccgg tcgtaacctt
    29341 tctcggccag ccagttacgc gcttcctggc tcacttccag agaaacacct ttctgatcca
    29401 gctgaacctg caactcgacg atgaatttat ccaccacctg atggatcacg tcggttgaca
    29461 gatgatcaaa ccagataatg ttgtcgagac ggttacggaa ttccggtgta aagatcttct
    29521 tgatctcctc catcgcatcg gtgctgttat cctggtggat aagaccaatg gatttacgct
    29581 cagtttcacg taccccggcg ttggtggtca tcaccagcac cacgttacgg aagtccgctt
    29641 tgcgtccgtt gttatcggtc agcgtaccgt tgtccatcac ctgcaacaga atattgaaca
    29701 cgtccggatg cgctttctcg atttcgtcca gcaacagcac cgcatgtgga tgcttgatga
    29761 ccgcatcagt cagcaaacct ccctgatcaa aaccaacgta tcccggaggc gcaccaatca
    29821 gacggctgac ggtatggcgt tccatatact cggacatatc aaagcgcagc agctcaatgc
    29881 ccaacgcttt cgaaagctgt accgtcacct ctgttttccc gaccccggta gggccggcaa
    29941 acaggaacga accaaccggt ttatgttcgt gacctaaacc tgcacgcgcc atcttaatgg
    30001 cttcagtcag cgcctcaatg gctttatcct gaccgaagac cagcattttc aggcgatcgc
    30061 cgaggttttt cagggtatcg cggtcactct gagaaacact cttctctgga atgcgcgcaa
    30121 tacgggccac cacggactca atatccgcca cattaacggt tttcttgcgt ttgcttaccg
    30181 gcatcaggcg tgcgcgagcg cccgcttcgt cgataacgtc aatggcttta tccggcagat
    30241 gacgatcgtt aatgtatttc accgccagct ctaccgctgc acgcaccgct tttgcggtat
    30301 aacgcacgtc gtggtgcgct tcatacttcg gtttcaggcc attgatgatt tgaacagttt
    30361 cttcgatcga cggttcagta atatcaattt tctggaagcg acgcgccaga gcacggtctt
    30421 tctcgaaaat gttgctgaac tcctgatagg ttgtcgaacc aattacgcga attttaccgc
    30481 tggagagcaa cggtttgatc aggttagccg catcgacctg gccaccagac gctgcacccg
    30541 caccgataat ggtgtggatc tcatcaataa acaggatgct gttagtgtcc tgctccagct
    30601 gcttgagcaa cgctttaaaa cgtttttcaa agtcgccgcg atatttagtg cccgctaaca
    30661 gagaaccgat atcgagagag taaatcgtac agtcagccat cacttccggc acatcgccct
    30721 gaacaattcg ccaggcaaga ccttccgcaa tcgcggtttt accgacacca gattccccca
    30781 ccagcagcgg gttgttttta cggcgacggc agagaacctg aatagcacgc tccagctcct
    30841 tctcacgacc aatcagtggg tcgattccgc ccacgcgcgc aagctgattc aggttcgtcg
    30901 tgaaattctc catacgttcc tccccaccag cttgttcttc gctgtttggc tggctgccag
    30961 gatcagaaga ctgtgtcggc tcgtctttac gcgtgccatg agagataaag ttcaccacat
    31021 cgagacggct gacttcatgt ttacgcaaca gatatgccgc ctgcgactcc tgttcgctaa
    31081 agatagcgac cagaacgttt gcaccggtta cctcattgcg accggaggac tggacatgga
    31141 agaccgcacg ttgcagtaca cgctgaaaac tcagcgtcgg ctgtgtgtcg cgctcctctt
    31201 cactggcagg cagaacgggt gtggtttgtt caataaaggc ttccagttcc tgacggagcg
    31261 caaccaaatc cacagaacac gcttccagcg cctcccgggc agatgggtta ctgagcagcg
    31321 ccagtaacaa gtgctcgacg gtcataaact catgacggtg ctcgcgcgct ctggcgaaag
    31381 ccatatttaa actgagttcc agttcttgat tgagcatagg cacctccccc aatttttatg
    31441 cctgcattca ggctttttct agcgtacaca gcaatggatg ctcattctcc ctcgcgtact
    31501 tgttcaccat cgccactttg gtttctgcaa cctcggcggt aaagactccg caaatggcct
    31561 tcccctggta gtgaacagcg agcatcaatt gcgttgcacg ttctacatca taagaaaaga
    31621 atttttgtaa cacgtcaata acaaactcca tcggagtgta atcatcattg actaatatca
    31681 ctttatacat agatggcggt tttagcgcgt cgcgaacttt ttcttccgcc agttgatcaa
    31741 agtccagcca gtcgttcgtt ttacccattg tcagttatca tcttcggtta cggttatcgg
    31801 tagaacgccc tgccgctgac aagagcttat ttgcctgacc aatctactcc acatgataga
    31861 taactatcat ctatcgttgg catcagcgac atctgtcaca ttcctgtcaa tagcgttaac
    31921 tgcttcaaat ttttgattca tacctgcccc ctggcccctg tcaaacgctt gacggctcgc
    31981 cctaattctc taaattgtat ttctagagtt ggcgaggttt tgaacagccc cctctctgac
    32041 cccggtttat tccatcttac ttgtataaga tttgcgaagg atgtcgaagc atggaaaagg
    32101 gtactgttaa gtggttcaac aatgccaaag ggtttggttt catctgccct gaaggcggcg
    32161 gcgaagatat tttcgctcat tattccacca ttcagatgga tggttacaga acgctaaaag
    32221 ctggacaatc cgttcagttt gatgtccacc aggggccaaa aggcaatcac gccagtgtta
    32281 ttgtgcccgt cgaagtagaa gcggcagtcg catagctctt ctgtctcatt gtgtacatcc
    32341 taaaggcaaa atgccagccc gatcggctgg catttttatc tcaaaaatta ctctcgtgcc
    32401 agagcatcta ctggatccag tcgtgccgca tttcgtgcgg gtaaccatcc aaataaaatc
    32461 ccggtgaccg tcgagcagag aaacgccagc agcagtgcca acggtgaaaa accaatctcc
    32521 cagccgggta agaaaagctg caaggtgaaa gcaattaaca gtgacagtgt tattcccaac
    32581 gcgccaccga ccaggcaaac cagtacggct tcgatcagga actgttgcaa aacatcgctt
    32641 gctcgcgcac ctacagccat gcgaatgcca atttcccgcg tccgctcggt cactgacacc
    32701 agcataatat tcattacacc aataccgccc accaccagcg aaatcaccgc caccagcgtc
    32761 agaaacagtt gtaaagtacg tgtggtcttt tcaacagttt tcaagacgcc gtccatgttc
    32821 caggtaaaga aatccttctt tccgtggcgc agtgaaagta aacgcgtgag ttgctgttcc
    32881 gcctcggcgc tgtcaaatcc ttcttttacc ctgacagtaa tggagttcag ccacgactgg
    32941 cccataactc gcccggacat cgtgctgtaa ggtagccaga cacgcagcac tttactgcta
    33001 ccaaacatcg actgtttttc ttccgccaca ccaatgactc tggcgggcat attgccgacc
    33061 agaatcacct cgccaaccac atctgcttta tgggggaaaa gctggcggcg agtattactg
    33121 tcgagaacca cgacctgcgc acgaccgttc agctgctcct gattaaaggt gtttccttca
    33181 ctgaaggtca tgccatagac attaaaataa tcgccgctca cgccattggc actggcagca
    33241 acatcaacat tgttataacg caggcgcagg ttttgcgaga cggcaggtgt ggctgaggcg
    33301 acccacggtt gtttttggat ggcgattaag tcgtcgtact tcagcgcctg ctgatattgc
    33361 ggatcgtcat cgccaaaatc tttcccggga tagacatcaa tagtattcgt accaatagaa
    33421 cgaatatccg ccagcaccat ttgtttggcg gcgtcaccca ccacgacaat ggaaaccacc
    33481 gacgcaatac cgataataat ccccagcatg gtcagtaaag tacgcatttt attcgctgcc
    33541 agcgcccgcc atgccatcgt cagcgcctcg ttaaaaccgc tgacaaactg ccgccagcca
    33601 gacaccgtgt tgacaacagg ctccgtcccg ccagcaacat tcactttttc aatggcggga
    33661 ggattgcgca caatttcgcc gtcgcgaatt tcgatcaccc gctcggcctg agcagcgacc
    33721 tgcggatcgt gggtgacgat aatcaccgta tgcccacgat cgcgcagctg atgcaggatc
    33781 gccatcacct cttcgccaga atggctgtcc agtgcgccgg ttggttcatc ggcaagaatt
    33841 acctgaccac cgttcatcaa tgcccgcgcg atgctgacgc gctgttgctg accacccgaa
    33901 agctgtgccg gataatactc tgtacggtct tccagcccca gccgttgcag caactcctgg
    33961 gcacgaagca gtcgctgttt ccgctcaaga ccagcataga cggcgggtac ttcaacgttc
    34021 tgctcggcgg ttaaatgcga aagtaaatgg taacgctgga aaataaagcc gaaatgctcg
    34081 cggcgcagtt gcgccagcgc atcggcgtcc agtgtggcaa catcctgacc ggcgacgcga
    34141 taggtgccgc tggtggcctt atccagacag ccgagaatat tcatcagggt cgatttaccg
    34201 gaacctgaag cgccaacaat cgctaccatc tcaccggcat aaatatcgag ggtgatgccc
    34261 ttcagcacct caacctgctc atcaccggca ggatagctgc gacgaatatc ctttaattcg
    34321 agcaaaggcg tcattgtgca gctcctggtt tggcctcacc aatcaccact tcatcgcccg
    34381 cttcaagccc tttgacaatc tcaacatcgg tatcgttacg tgcgccaatc gtcacttcac
    34441 gctcgcgtgt ttcaccatta cgcaacaatt tgactttata acgattatcg ccaaccggat
    34501 cgcctaacgc cgacagaggg atcgtcagca catttttcac atcggtgagc tgaatatgca
    34561 cttgcgcagt catatccagc cgcagcaaac cattggggtt ggggacttca aaacgggcgt
    34621 aatagaaaat agcgtcgtta accttttccg gcgtcggtag tacatccttg atttgcccct
    34681 cgtagcgcgt cagtggatcg ccaagtaccg taaaccaggc tttttgcccc ggcttcaggt
    34741 ggattacatc cgcttcagaa acctgcgctt ttaccagcat ggtgctcata tctgccagcg
    34801 tcagaatgtt cggtgcttgt tgtgcggcaa tcaccgtctg gccttgcaga gtggtgattt
    34861 gcgtgacttc cccggccatc ggggcaacga tgcgagtgta atcgagattg gttttagccg
    34921 tatcgagaga ggcctgattg cgcttgattt gcgcgtcaat ggtgccaatt tgcgcctgtt
    34981 tcacagccat ctccgtcgcg gcggtgtcga gatcctgctg tgaaaccgcc tgcgtttgtg
    35041 ccagacgttg ctgacgggaa tacgtcaccc gcgccagttt cagctccgct tccgcctgct
    35101 gccgctgcgc acgtagctcc atcagcgttg cttcgacctc cttaatctgg ttttcagcct
    35161 gttcaggatc aataacccct aaaagctggt ctttttttac tttatcgcca atcgccaccg
    35221 acagagtttt caactgaccg ctgacctgcg cgcccacgtc aaccttacgc agcgcgtcca
    35281 gctttccggt cgccagcacg ctttgctgta aatcaccggg gcgcacaatc agtgtctgat
    35341 aagtcggcac gggtgcgtta agaattctcc ataacgtaat cagtccggcg atgactatca
    35401 ccagcgcaat aacgtaacgc ttcttcacgg ttttccgctt tttcataaat attctgaata
    35461 ctccatatgc caatccgggt cattaacttc aacgaaatat caacaaaacc ctaaaattta
    35521 cgttagtaat cacactattg atgtttggtt aagatacgca atggtgaaat cgacgtcatg
    35581 tataaccatt gatttcatga atatgtcgca gctaactgaa cggaccttta cgccatctga
    35641 atctctcagc agcctgtcac tttttcttag tctggcacgt ggacagtgtc ggccgggtaa
    35701 attctggcat cgccgtagtt ttcgccagaa atttttgctg cgctcgttga ttatgccgcg
    35761 tttaagcgtt gagtggatga acgaactttc ccactggcct aatctcaatg tgttgttaac
    35821 gcgccaaccg cgactgcctg tgcgtctgca tcgcccttac cttgcggcga atcttagccg
    35881 taagcaattg ctggaggcgt tacgttacca ttatgcgtta ctccgcggat gtatgtcggc
    35941 ggaagaattc agcttatatt tgaatacccc cgggctgcaa ctggctaaac tggaaggcaa
    36001 aaacggcgag cagttcacgc ttgagctgac catgatgatc tcaatggata aagaaggtga
    36061 cagcacaatc ctgttccgca acagcgaagg tattcctctg gcagagctca cttttaccct
    36121 gtgtgaatat caggggaaaa gaacgatgtt tattggcgga ctgcaaggcg caaaatggga
    36181 aatcccacat caggaaatcc agaatgcgac gaaagcctgc cacgggctat ttcccaaacg
    36241 cctcgtgatg gaagcggcct gtctgtttgc ccaacgtttg caggtagagc agattattgc
    36301 cgtcagcaat gaaacgcata tttaccgcag cctgcgttat cgtgataaag aaggcaagat
    36361 ccatgccgat tacaacgctt tctgggaatc ggttggcggc gtatgtgatg ctgaacgcca
    36421 ttaccgcctt ccagcacaga tagcacgaaa agagattgcc gaaatcgcca gtaaaaaacg
    36481 ggctgaatac cgtcggcgct atgagatgct cgacgctatt cagccacaaa tggccacgat
    36541 gtttcgcggt taatccgcgc gaccgcgcgc cagccacagc acgcgtgaga acattttttt
    36601 cagcagcgtc ggtacggagt ccacaccacg acgtccggcc tccattgcca cttcaatggc
    36661 aagatcgggt ttggaagagc tatggatcgc tttcgagata attttgcgca gattcatcgg
    36721 tacattttcc gggatttgcg ccacgcggtg gaacacatcg gaaaatccct ggcgatacat
    36781 aaaatgttcc atatccagcg ccggtaacgc cgttaaatgt tctcgttcgg cttcccgatc
    36841 gttattcaac aggctgcgta ccgtagcggc atatttcttc cctgcttcat cgccatcgac
    36901 cagtacatgc cattcaatcc ccattcggcg ggcaaattta accagtggct ttagcccgga
    36961 ctgggcaaac tcaatgacct tgatcccttc ggcatcgaaa tggtgtccac actggcgcgc
    37021 cagttcattg ataacccagg tttccgtttc cccttccacc agcaaccagc agcgagcaaa
    37081 cagcgatgac ggacggttaa aacgaatgtg aaaggatatg cgtcggctat cttcggtact
    37141 caagccactc ggccccagac gccaggcggc aacgcgcgag gactcacgta ccagtcggca
    37201 aacatgctct accggcgtta acgaaagcaa ctcacccgag ttggtggtgg caatgcgctg
    37261 caatggcaga agattcaaca gatgccaggc aactgaaagc ataatggggt gtaaacgggt
    37321 ttctggatct tcaattagca ataacggacg cgcgtcttta tccagtctca atgtgccttt
    37381 tgcctgcaat aaagtagcaa acaggccgag caaaataacc cgatacgagc gccctccagg
    37441 tcggtcaatc atccggttga tgatgtccag atagcgccag cttcgttgct cattgctggc
    37501 ccgccgccgc attaaacgat atcgcgcctg tccagccccc tgctcagaga aataatgctc
    37561 aagcaactgc accattgcgg aaagtccctg acgaatctgt ccatcagaga gattttgcgg
    37621 atgtgaggac aactcacggg cgaggaaatc gagctggcgc gcggtgactt ccacattggg
    37681 aacatttggc accgtgccgt tacgaatacg gcgcataaaa cgggcatcac gcaagcgcag
    37741 caccggcatt aaacgcacca gatggcgtgc ctgatcgtta atatcctcga catcaatcgg
    37801 atgtccgtct ttatcgagaa aactgcgcag tgtcatcacg ctgccgtctt ccgcactctc
    37861 cccttccaga cgataaaaaa tacggtgata gccatcggtg catggcgtcc agcacgcttc
    37921 cagcggccga taacggcgaa cccgatgtcg gcctggcagc gattcgcgga aggtcaaaat
    37981 aatatgcaga tgatgttctc gcccgttgat atctcccggc gggaaccaga aatcgtcgcg
    38041 ctcaaaatgg tagagatctg attctggcga tagcagcagg gttaaggcgt ccagcaagct
    38101 ggatttaccc cacgcgttct ccccaatcag gacgttgttt tgttcgagca tcaacgacaa
    38161 acggttgata ccgcgaaaac ccacaatttc aacgcgctca agaatcatat cccctccaga
    38221 aatatgtgac ttatccttta cgctggcagt ataacgggaa aggccttgct aatacgtgat
    38281 gacatcacat ttaagcatgg caaatgtctg aaaaatgagc gaataatcgc gttatactcc
    38341 ctcttgctga taagtctgca aaaacaacaa aatcaaataa ttagcatgat aaattatttt
    38401 ttattacgcc taaaggatta aatcttttgc ttgcactaca ctgtcaatca gacaaatgac
    38461 tatatggggc aacccgctgt tgccctaaat caaatttact ggatttattt atctggtgaa
    38521 tgactcgcct tcgtttttaa aatagccgcg cattatttcc gtcgtcactt ttttacgacg
    38581 accctataaa acgaccatat ttttcacagg gtcaattttt aattgaggtg gatatgttca
    38641 gaaaattagc agctgaatgt tttggtactt tctggcttgt ttttggtggc tgtggtagtg
    38701 ctgtactggc cgcaggcttc ccggaattag gcattggttt tgccggcgtg gcgttggcgt
    38761 tcggtctgac cgttctgacg atggcctttg ctgttggtca tatttctggt ggtcatttta
    38821 acccggcggt cactattggt ttatgggctg gcggacgttt tccggcaaaa gaagtcgttg
    38881 gctacgtaat tgcccaggtt gtcggcggta ttgttgcagc ggcgctgctg tatttaattg
    38941 ccagtggtaa aacgggtttt gacgcggcag ccagcggttt tgcttctaac ggttatggcg
    39001 agcattcacc aggcggttat tccatgcttt ccgcgctggt agttgaactg gtattgagtg
    39061 caggtttcct gttggtgatc cacggcgcaa ccgacaaatt cgcgccggca ggttttgcgc
    39121 cgatcgctat tggtctggcc ttaaccctga ttcacttaat tagtattccg gtgactaaca
    39181 cttctgttaa cccggcgcgc agcaccgcgg ttgctatctt ccagggcggc tgggcattag
    39241 aacaactgtg gttcttctgg gtggtgccaa ttgtcggcgg cattatcggt ggtctgattt
    39301 accggaccct gctggaaaag cgtgattaat taacgcagaa ggcctggcgt aatgtcaggc
    39361 ctttttttaa gatgttgtta tatgttgatt catcatcttg ttgcgctcct ttcatgagct
    39421 aagccacata ttgccactgg cgcaaggagc gcgcaggggg cggccaatcg ccgccgcccc
    39481 atgcaccccc gggctctggc gaacaaaatc gccgctgcgc ggtgccctcg acttatccct
    39541 tacggctacc gggtcgggcg cgaggtaaca tccctgtaaa acgcgccctc agcccacatc
    39601 catgtgggct gccccggcct tcagggaacg cctcggcgat tttgacgcca ccaacatcgg
    39661 tgcagtttgt aattaaagcg caagaatatc tttaaatgaa aacgaatact ctactgctcc
    39721 ttatgcataa catttccttt taattgcaac tttactcgtc cttccgcttt cagtagtgtg
    39781 tcatcgcgca tttcgacttc atctttgcaa ggactgggtt ttcatgtttt ctgggctgtt
    39841 aatcattctg gttcccctga ttgtgggtta cctcattccg cttcgccaac aagctgcgtt
    39901 aaaagttatt aatcagctat taagctggat ggtttacctt attctctttt ttatgggtat
    39961 aagtctggcg ttcctcgata acctcgccag taacctgttg gcgattctgc attattctgc
    40021 cgtcagtatt accgttattt tactgtgtaa tattgccgcc ctgatgtggc tggagcgagg
    40081 cctgccgtgg cgcaaccacc atcagcaaga aaaactcccg tcgcgtattg cgatggcgct
    40141 ggagtcgcta aaactgtgcg gcgtagtagt gattggtttt gccattggtc tgagtggact
    40201 ggctttctta caacacgcga ccgaagccag tgaatacacg ttaattttgc tacttttcct
    40261 cgttggtatt cagttgcgca ataatggcat gaccttaaag cagattgtcc ttaatcgccg
    40321 gggaatgatt gtcgccgtgg tggtggttgt cagttcatta attggtggtt taattaacgc
    40381 ctttattctt gatctcccca tcaatacctc gctggcaatg gcctccggtt tcggctggta
    40441 ttctctttcc ggtattttat tgaccgaatc ttttggtccg gtaatcggga gcgcggcgtt
    40501 ttttaatgat ctggcccgtg aactgattgc tattatgttg atccctgggc tgattcgccg
    40561 cagccgctct actgcactgg gcttatgcgg tgccacatca atggatttca ccctgcccgt
    40621 tcttcaacgt actggcgggc tggatatggt cccggcggca attgttcacg gttttattct
    40681 tagcctgtta gtgccgatcc tcatcgcctt tttctctgcg taatacctct ctggcggtag
    40741 attcctgccg ccaaaattgc gctaaatcaa tctcccttaa agttgcatga aaaatccctt
    40801 ttatccccgc gttaagcgtc ttaaccttaa acatgtatat taaatataac tttaaaaggt
    40861 gtgatcatgt tttgtgtgca atgtgaacaa actatccgta ctccggcagg aaacggctgc
    40921 tcatacgcgc aggggatgtg tggtaaaacg gcggaaactt ctgaccttca ggatttactc
    40981 atcgcggcgc tgcaagggct ttctgcctgg gcggtaaaag cgcgtgaata cggcatcatc
    41041 aaccacgatg ttgacagctt cgcgccacgt gcctttttct caactctgac caacgttaac
    41101 ttcgattctc cgcgtattgt cggctacgct cgtgaagcga ttgccctgcg cgaggcgctg
    41161 aaagcgcaat gcctggctgt agatgccaac gcgcgcgtcg ataacccgat ggctgacctg
    41221 caactggtga gcgacgatct cggcgagctg caacgtcagg ctgcagaatt tacccctaac
    41281 aaagataaag cggcgattgg cgaaaacatt ctcggcctgc gtctgctgtg cctgtatggc
    41341 ctgaaaggtg cggcggccta tatggaacac gcgcatgttc tcggtcaata cgacaacgat
    41401 atttatgccc agtatcataa aatcatggcg tggctgggga cctggcctgc cgatatgaac
    41461 gcgcttcttg agtgttcaat ggaaatcggc cagatgaact tcaaagtgat gagcattctg
    41521 gatgcaggcg aaaccggtaa atacggtcac ccgacgccaa cccaggtcaa cgtcaaagcg
    41581 acggcgggta aatgcattct gatttccggt cacgatctca aagatctcta caacctgctg
    41641 gaacagaccg aaggcacggg cgttaatgtc tacacccacg gcgaaatgct gcctgcgcat
    41701 ggctacccgg agctgcgtaa attcaagcat ctggtcggta actacggcag tggctggcag
    41761 aatcagcaag tggagttcgc tcgtttccct ggccccatcg tgatgacctc gaactgcatc
    41821 atcgacccaa ccgtaggcgc ttatgacgat cgtatctgga cccgcagcat tgttggctgg
    41881 cctggcgtgc gtcatctgga cggtgatgat ttctctgcgg ttatcaccca ggcgcaacag
    41941 atggcaggct tcccgtacag cgaaattccg caccttatca ccgtgggttt tggtcgccag
    42001 acgctgcttg gcgctgctga tacgctgatt gatctggtga gccgtgaaaa actgcgtcat
    42061 atcttcctgc ttggtggctg tgacggcgca cgcggcgagc gccactactt caccgatttc
    42121 gccaccagcg tgccggatga ctgcctgatc ctgaccctcg cctgtggtaa atatcgcttt
    42181 aacaaacttg agtttggcga tatcgaaggt ctgccgcgtc tggtagatgc aggtcaatgt
    42241 aacgatgctt actcagcgat tattctggct gtcactctgg cagagaaact gggctgcggt
    42301 gtcaacgatc tgccgctgtc gctggtgctc tcctggtttg aacagaaagc gatcgtcatt
    42361 ctgctgacgc tgctttctct gggcgtgaaa aatatcgtca ccggcccgac tgcacctggt
    42421 ttcctgacac cagacctgct ggcggtgctg aacgagaaat ttggcctgcg ttctatcacc
    42481 actgttgaag aagacatgaa gcaactgttg agcgcgtaag gagtttgtcg atgacgatgc
    42541 caacgaatca atgcccgtgg cggatgcagg ttcatcacat tacgcaagaa acgccggatg
    42601 tgtggacgat ttccctgatt tgccacgatt actacccata tcgcgccggg caatatgcac
    42661 tggtcagcgt gcgaaactca gcggaaacgc tgcgtgctta caccatttcc tccacgccag
    42721 gcgtgagtga atatatcacc ctgaccgtgc ggcggattga tgacggtgtc ggctcccagt
    42781 ggctgacgcg cgatgtaaaa cgcggtgatt atctctggct ttcggacgcg atgggggaat
    42841 ttacctgcga cgataaagca gaagataaat tcctgttgct ggcggcaggc tgcggcgtca
    42901 cgccgattat gtcgatgcgt cgctggcttg cgaagaaccg tccacaggcc gatgtgcggg
    42961 tgatctacaa cgtgcgtacg ccgcaggatg ttattttcgc cgatgagtgg cgtaactatc
    43021 cggtaacgct ggtggcagaa aataacgtta ccgaaggctt tatcgctggt cgtctcactc
    43081 gcgaactgct ggcaggtgta cctgacttag cttcacgtac cgtgatgacc tgcggcccgg
    43141 ctccgtatat ggattgggta gagcaggaag tgaaagcgct cggcgtgacg cgtttcttta
    43201 aagagaaatt cttcacccca gtagcagaag cggcgaccag cggtctgaaa ttcaccaaac
    43261 tgcaaccggc acgagaattt tacgccccgg ttggcaccac gctactggag gcgctggaaa
    43321 gcaataacgt tccggttgtc gctgcctgcc gtgcgggtgt ttgcggctgc tgtaagacaa
    43381 aagtggtttc cggtgaatat acggtgagca gtacaatgac gctgaccgac gccgaaatcg
    43441 ctgaaggtta cgtactggcc tgctcctgcc atccgcaggg ggatttggtt ctcgcataat
    43501 cgccttatgc ccgatgatat tcctttcatc gggctattta accgttagtg cctcctttct
    43561 ctcccatccc ttccccctcc gtcagatgaa ctaaacttgt taccgttatc acattcagga
    43621 gatggagaac catgaaacaa acggttgcag cttatatcgc caaaacactc gaatcggcag
    43681 gggtgaaacg catctgggga gtcacaggcg actctctgaa cggtcttagt gacagtctta
    43741 atcgcatggg caccatcgag tggatgtcca cccgccacga agaagtggcg gcctttgccg
    43801 ctggcgctga agcacaactt agcggagaac tggcggtctg cgccggatcg tgcggccccg
    43861 gcaacctgca cttaatcaac ggcctgttcg attgccaccg caatcacgtt ccggtactgg
    43921 cgattgccgc tcatattccc tccagcgaaa ttggcagcgg ctatttccag gaaacccacc
    43981 cacaagagct attccgcgaa tgtagtcact attgcgagct ggtttccagc ccggagcaga
    44041 tcccacaagt actggcgatt gccatgcgca aagcggtgct taaccgtggc gtttcggttg
    44101 tcgtgttacc aggcgacgtg gcgttaaaac ctgcgccaga aggggcaacc acgcactggt
    44161 atcatgcgcc acaaccagtc gtgacgccgg aagaagaaga gttacgcaaa ctggcgcaac
    44221 tgctgcgtta ttccagcaat atcgccctga tgtgtggcag cggctgcgcg ggggcgcata
    44281 aagagttagt tgagtttgcc gggaaaatta aagcgcctat tgttcatgcc ctgcgcggta
    44341 aagaacatgt cgaatacgat aatccgtatg atgttggaat gaccgggtta atcggcttct
    44401 cgtcaggttt ccataccatg atgaacgccg acacgttagt gctactcggc acgcaatttc
    44461 cctaccgcgc cttctacccg accgatgcca aaatcattca gattgatatc aacccagcca
    44521 gcatcggcgc tcacagcaag gtggatatgg cactggtcgg cgatatcaag tcgactctgc
    44581 gtgcattgct tccattggtg gaagaaaaag ccgatcgcaa gtttctggat aaagcgctgg
    44641 aagattaccg cgacgcccgc aaagggctgg acgatttagc taaaccgagc gagaaagcca
    44701 ttcacccgca atatctggcg cagcaaatta gtcattttgc cgccgatgac gctattttca
    44761 cctgtgacgt tggtacgcca acggtgtggg cggcacgtta tctaaaaatg aacggcaagc
    44821 gtcgcctgtt aggttcgttt aaccacggtt cgatggctaa cgccatgccg caggcgctgg
    44881 gtgcgcaggc gacagagcca gaacgtcagg tggtcgccat gtgcggcgat ggcggtttta
    44941 gcatgttgat gggcgatttc ctctcagtag tgcagatgaa actgccagtg aaaattgtcg
    45001 tctttaacaa cagcgtgctg ggctttgtgg cgatggagat gaaagctggt ggctatttga
    45061 ctgacggcac cgaactacac gacacaaact ttgcccgcat tgccgaagcg tgcggcatta
    45121 cgggtatccg tgtagaaaaa gcgtctgaag ttgatgaagc cctgcaacgc gccttctcta
    45181 tcgacggtcc ggtgttggtg gatgtggtgg tcgccaaaga agagttagcc attccaccgc
    45241 agatcaaact cgaacaggcc aaaggtttta gcctgtatat gttgcgcgca attatcagcg
    45301 gacgcggcga tgaagtgatc gaactggcga aaacgaactg gctaaggtaa aaagggtggc
    45361 atttcccgtc ataataagga catgccatga ttgatttacg cagtgatacc gttacccgac
    45421 cgagccgcgc catgctcgaa gcgatgatgg ccgccccggt tggggacgac gtttacggag
    45481 acgaccctac cgttaatgct ctgcaggact atgcagcaga gctttccggt aaagaagccg
    45541 ccatttttct gcctaccggc actcaggcca acctggtcgc tctgctcagt cactgcgaac
    45601 gtggcgaaga gtatattgtc ggtcaggccg cgcataacta tctgtttgaa gccggtggcg
    45661 cagcggtgct gggcagtatt cagccgcaac ccatcgacgc ggctgccgac ggcacgctac
    45721 cgctggataa agtggcgatg aaaatcaaac ccgacgatat ccatttcgcc cgcaccaaat
    45781 tactcagtct ggaaaacacc cacaacggca aagtgctgcc gcgtgaatac ctgaaagaag
    45841 catgggaatt tacccgcgag cgcaatctgg cgctgcatgt tgacggtgcg cgcatcttta
    45901 atgccgtggt ggcttacggc tgcgaactga aagagatcac gcaatattgt gattcgttca
    45961 ccatttgcct gtcgaaaggt cttgggacgc cagtcggttc attactcgtc ggtaatcgtg
    46021 attacattaa acgtgccatc cgctggcgga aaatggccgg tggcgggatg cgtcagtccg
    46081 ggattctggc tgccgccggg atgtatgccc tgaaaaataa cgtcgcacgg ttgcaggaag
    46141 atcacgacaa cgccgcctgg atggcggagc aactgcgtga agcaggcgcg gatgtgatgc
    46201 gtcaggacac taatatgctg tttgttcgcg tcggggaaga aaatgctgcc gcgttaggcg
    46261 aatacatgaa agcgagaaac gtgctgatta acgcctcgcc gattgtccgc ctggtgacgc
    46321 atcttgacgt ctcgcgcgaa caactggcgg aagtcgccgc ccactggcgc gcattcctgg
    46381 cgcgttaagg agagagacgt gccgcaacgc attttagttc tcggtgccag tggctacatt
    46441 ggtcagcatc tggtgcgcac actcagccag caagggcatc agatcctggc ggcggcacgt
    46501 catgtcgaca ggcttgcaaa gctgcaactg gcaaacgtca gttgccataa agtcgatctc
    46561 agctggccgg ataaccttcc ggccctgttg caggatatcg ataccgtcta ttttctggtg
    46621 cacagcatgg gcgaaggcgg cgattttatc gctcaggagc gccaggtggc tctcaacgtc
    46681 cgcgatgcgc tacgtgaagt accagttaag caattaatct ttctcagttc gttgcaggcc
    46741 ccgccacatg agcagtcgga tcatctgcgt gctcgtcagg ctacggcgga cattcttcgt
    46801 gaagcgaatg tacctgtgac cgaacttcgg gccggaatta tcgttggcgc aggttcagcg
    46861 gcgttcgaag tcatgcgcga tatggtctac aacctgccag tgttaacgcc gccacgctgg
    46921 gtacgttcac gcaccacgcc catcgcgctg gaaaacttgc tgcactatct ggtggcgttg
    46981 ttagatcatc cggccagcga acaccgcatc ttcgaagccg ccggaccaga ggtgctcagt
    47041 tatcagcaac agtttgaaca ttttatggcg gtgagcggta
//
LOCUS       DAZKHF020000009        44485 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2201S31241
            SAMN43267800-rid25106413.denovo.009, whole genome shotgun sequence.
ACCESSION   DAZKHF020000009 DAZKHF020000000
VERSION     DAZKHF020000009.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267800
            Sequence Read Archive: SRR30307263
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 44485)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 44485)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 44485)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350581.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350581.2
            Genome Coverage       :: 151x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:21:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,652
            CDSs (total)                      :: 5,557
            Genes (coding)                    :: 5,320
            CDSs (with protein)               :: 5,320
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 237
            CDSs (without protein)            :: 237
            Pseudo Genes (ambiguous residues) :: 0 of 237
            Pseudo Genes (frameshifted)       :: 102 of 237
            Pseudo Genes (incomplete)         :: 130 of 237
            Pseudo Genes (internal stop)      :: 52 of 237
            Pseudo Genes (multiple problems)  :: 41 of 237
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..44485
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267800-rid25106413.denovo.009"
                     /serotype="O 26:H11"
                     /isolate="2201S31241"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-01"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            complement(56..1420)
                     /gene="mnmE"
                     /locus_tag="ACHOSC_RS01925"
                     /old_locus_tag="pgaptmp_000385"
     CDS             complement(56..1420)
                     /gene="mnmE"
                     /locus_tag="ACHOSC_RS01925"
                     /old_locus_tag="pgaptmp_000385"
                     /EC_number="3.6.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis GTPase MnmE"
                     /protein_id="HGU4546398.1"
                     /translation="MSDNDTIVAQATPPGRGGVGILRISGLKAREVAETVLGKLPKPR
                     YADYLPFKDADGSVLDQGIALWFPGPNSFTGEDVLELQGHGGPVILDLLLKRILTIPG
                     LRIARPGEFSERAFLYDKLDLAQAEAIADLIDASSEQAARSALNSLQGAFSARVNHLV
                     EALTHLRIYVEAAIDFPDEEIDFLSDGKIEAQLNDVIADLDAVRAEARQGSLLREGMK
                     VVIAGRPNAGKSSLLNALAGREAAIVTDIAGTTRDVLREHIHIDGMPLHIIDTAGLRE
                     ASDEVERIGIERAWQEIEQADRVLFMVDGTTTDAVDPAEIWPEFIARLPAKLPITVVR
                     NKADITGETLGMSEVNGHALIRLSARTGEGVDVLRNHLKQSMGFDTNMEGGFLARRRH
                     LQALEQAAEHLQQGKAQLLGAWAGELLAEELRLAQQNLSEITGEFTSDDLLGRIFSSF
                     CIGK"
     gene            complement(1526..3172)
                     /gene="yidC"
                     /locus_tag="ACHOSC_RS01930"
                     /old_locus_tag="pgaptmp_000386"
     CDS             complement(1526..3172)
                     /gene="yidC"
                     /locus_tag="ACHOSC_RS01930"
                     /old_locus_tag="pgaptmp_000386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0032977 - membrane insertase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertase YidC"
                     /protein_id="HGU4546399.1"
                     /translation="MDSQRNLLVIALLFVSFMIWQAWEQDKNPQPQAQQTTQTTTTAA
                     GSAADQGVPASGQGKLISVKTDVLDLTINTRGGDVEQALLPAYPKELNSTQPFQLLET
                     SPQFIYQAQSGLTGRDGPDNPANGPRPLYNVEKDAYVLAEGQNELQVPMTYTDAAGNT
                     FTKTFVLKRGDYAVNVNYNVQNAGEKPLEISTFGQLKQSITLPPHLDTGSSNFALHTF
                     RGAAYSTPDEKYEKYKFDTIADNENLNISSKGGWVAMLQQYFATAWIPHNDGTNNFYT
                     ANLGNGIAAIGYKSQPVLVQPGQTGAMNSTLWVGPEIQDKMAAVAPHLDLTVDYGWLW
                     FISQPLFKLLKWIHSFVGNWGFSIIIITFIVRGIMYPLTKAQYTSMAKMRMLQPKIQA
                     MRERLGDDKQRISQEMMALYKAEKVNPLGGCFPLLIQMPIFLALYYMLMGSVELRQAP
                     FALWIHDLSAQDPYYILPILMGVTMFFIQKMSPTTVTDPMQQKIMTFMPVIFTVFFLW
                     FPSGLVLYYIVSNLVTIIQQQLIYRGLEKRGLHSREKKKS"
     gene            complement(3175..3432)
                     /gene="yidD"
                     /locus_tag="ACHOSC_RS01935"
                     /old_locus_tag="pgaptmp_000387"
     CDS             complement(3175..3432)
                     /gene="yidD"
                     /locus_tag="ACHOSC_RS01935"
                     /old_locus_tag="pgaptmp_000387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_462741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertion efficiency factor
                     YidD"
                     /protein_id="HGU4546400.1"
                     /translation="MAPPLSPGSRVLIALIRVYQRLISPLLGPHCRFTPTCSSYGIEA
                     LRRFGVIKGSWLTVKRVLKCHPLHPGGDDPVPPGPFDTREH"
     gene            complement(3396..3755)
                     /gene="rnpA"
                     /locus_tag="ACHOSC_RS01940"
                     /old_locus_tag="pgaptmp_000388"
     CDS             complement(3396..3755)
                     /gene="rnpA"
                     /locus_tag="ACHOSC_RS01940"
                     /old_locus_tag="pgaptmp_000388"
                     /EC_number="3.1.26.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014172301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000049 - tRNA binding [Evidence IEA];
                     GO_function: GO:0004526 - ribonuclease P activity
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease P protein component"
                     /protein_id="HGU4546401.1"
                     /translation="MVKLAFPRELRLLTPSQFTFVFQQPQRAGTPQITILGRLNSLGH
                     PRIGLTVAKKNVRRAHERNRIKRLTRESFRLRQHELPAMDFVVVAKKGVADLDNRALS
                     EALEKLWRRHCRLARGS"
     gene            complement(3772..3912)
                     /gene="rpmH"
                     /locus_tag="ACHOSC_RS01945"
                     /old_locus_tag="pgaptmp_000389"
     CDS             complement(3772..3912)
                     /gene="rpmH"
                     /locus_tag="ACHOSC_RS01945"
                     /old_locus_tag="pgaptmp_000389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001686914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L34"
                     /protein_id="HGU4546402.1"
                     /translation="MKRTFQPSVLKRNRSHGFRARMATKNGRQVLARRRAKGRARLTV
                     SK"
     gene            complement(4142..4222)
                     /locus_tag="ACHOSC_RS01950"
                     /old_locus_tag="pgaptmp_000390"
     CDS             complement(4142..4222)
                     /locus_tag="ACHOSC_RS01950"
                     /old_locus_tag="pgaptmp_000390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4546403.1"
                     /translation="MTIDKNWLNRSNKDPGRSLRFTHQPV"
     gene            4519..5922
                     /gene="dnaA"
                     /locus_tag="ACHOSC_RS01955"
                     /old_locus_tag="pgaptmp_000391"
     CDS             4519..5922
                     /gene="dnaA"
                     /locus_tag="ACHOSC_RS01955"
                     /old_locus_tag="pgaptmp_000391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003688 - DNA replication origin binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA];
                     GO_process: GO:0006275 - regulation of DNA replication
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromosomal replication initiator protein DnaA"
                     /protein_id="HGU4546404.1"
                     /translation="MSLSLWQQCLARLQDELPATEFSMWIRPLQAELSDNTLALYAPN
                     RFVLDWVRDKYLNNINGLLTSFCGADAPQLRFEVGTKPVTQTPQAAVTSNVAAPAQVA
                     QTQPQRAAPSTRSGWDNIPAPAEPTYRSNVNVKHTFDNFVEGKSNQLARAAARQVADN
                     PGGAYNPLFLYGGTGLGKTHLLHAVGNGIMARKPNAKVVYMHSERFVQDMVKALQNNA
                     IEEFKRYYRSVDALLIDDIQFFANKERSQEEFFHTFNALLEGNQQIILTSDRYPKEIN
                     GVEDRLKSRFGWGLTVAIEPPELETRVAILMKKADENDIRLPGEVAFFIAKRLRSNVR
                     ELEGALNRVIANANFTGRAITIDFVREALRDLLALQEKLVTIDNIQKTVAEYYKIKVA
                     DLLSKRRSRSVARPRQMAMALAKELTNHSLPEIGDAFGGRDHTTVLHACRKIEQLREE
                     SHDIKEDFSNLIRTLSS"
     gene            5927..7027
                     /gene="dnaN"
                     /locus_tag="ACHOSC_RS01960"
                     /old_locus_tag="pgaptmp_000392"
     CDS             5927..7027
                     /gene="dnaN"
                     /locus_tag="ACHOSC_RS01960"
                     /old_locus_tag="pgaptmp_000392"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006177590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit beta"
                     /protein_id="HGU4546405.1"
                     /translation="MKFTVEREHLLKPLQQVSGPLGGRPTLPILGNLLLQVADGTLSL
                     TGTDLEMEMVARVALVQPHEPGATTVPARKFFDICRGLPEGAEIAVQLEGERMLVRSG
                     RSRFSLSTLPAADFPNLDDWQSEVEFTLPQATMKRLIEATQFSMAHQDVRYYLNGMLF
                     ETEGEELRTVATDGHRLAVCSMPIGQSLPSHSVIVPRKGVIELMRMLDGGDNPLRVQI
                     GSNNIRAHVGDFIFTSKLVDGRFPDYRRVLPKNPDKHLEAGCDLLKQAFARAAILSNE
                     KFRGVRLYVSENQLKITANNPEQEEAEEILDVTYSGAEMEIGFNVSYVLDVLNALKCE
                     NVRMMLTDSVSSVQIEDAASQSAAYVVMPMRL"
     gene            7027..8100
                     /gene="recF"
                     /locus_tag="ACHOSC_RS01965"
                     /old_locus_tag="pgaptmp_000393"
     CDS             7027..8100
                     /gene="recF"
                     /locus_tag="ACHOSC_RS01965"
                     /old_locus_tag="pgaptmp_000393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication/repair protein RecF"
                     /protein_id="HGU4546406.1"
                     /translation="MSLTRLLIRDFRNIETADLALSPGFNFLVGANGSGKTSVLEAIY
                     TLGHGRAFRSLQIGRVIRHEQEAFVLHGRLQGEERETAIGLTKDKQGDSKVRIDGTDG
                     HKVAELAHLMPMQLITPEGFTLLNGGPKYRRAFLDWGCFHNEPGFFTAWSNLKRLLKQ
                     RNAALRQVTRYEQLRPWDKELIPLAEQISTWRAEYSAGIAADMADTCKQFLPEFSLTF
                     SFQRGWEKETEYAEVLERNFERDRQLTYTAHGPHKADLRIRADGAPVEDTLSRGQLKL
                     LMCALRLAQGEFLTRESGRRCLYLIDDFASELDDERRGLLASRLKATQSQVFVSAISA
                     EHVIDMSDENSKMFTVEKGKITD"
     gene            8129..10543
                     /gene="gyrB"
                     /locus_tag="ACHOSC_RS01970"
                     /old_locus_tag="pgaptmp_000394"
     CDS             8129..10543
                     /gene="gyrB"
                     /locus_tag="ACHOSC_RS01970"
                     /old_locus_tag="pgaptmp_000394"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005694 - chromosome [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase (ATP-hydrolyzing) subunit B"
                     /protein_id="HGU4546407.1"
                     /translation="MSNSYDSSSIKVLKGLDAVRKRPGMYIGDTDDGTGLHHMVFEVV
                     DNAIDEALAGHCKEIIVTIHADNSVSVQDDGRGIPTGIHPEEGVSAAEVIMTVLHAGG
                     KFDDNSYKVSGGLHGVGVSVVNALSQKLELVIQREGKIHRQIYEHGVPQAPLAVTGET
                     EKTGTMVRFWPSLETFTNVTEFEYEILAKRLRELSFLNSGVSIRLRDKRDGKEDHFHY
                     EGGIKAFVEYLNKNKTPIHPNIFYFSTEKDGIGVEVALQWNDGFQENIYCFTNNIPQR
                     DGGTHLAGFRAAMTRTLNAYMDKEGYSKKAKVSATGDDAREGLIAVVSVKVPDPKFSS
                     QTKDKLVSSEVKSAVEQQMNELLAEYLLENPTDAKIVVGKIIDAARAREAARRAREMT
                     RRKGALDLAGLPGKLADCQERDPALSELYLVEGDSAGGSAKQGRNRKNQAILPLKGKI
                     LNVEKARFDKMLSSQEVATLITALGCGIGRDEYNPDKLRYHSIIIMTDADVDGSHIRT
                     LLLTFFYRQMPEIVERGHVYIAQPPLYKVKKGKQEQYIKDDEAMDQYQISIALDGATL
                     HTNASAPALAGEALEKLVSEYNATQKMINRMERRYPKAMLKELIYQPTLTEADLSDEQ
                     TVTRWVNALVSELNDKEQHGSQWKFDVHTNAEQNLFEPIVRVRTHGVDTDYPLDHEFI
                     TGGEYRRICTLGEKLRGLLEEDAFIERGERRQPVASFEQALDWLVKESRRGLSIQRYK
                     GLGEMNPEQLWETTMDPESRRMLRVTVKDAIAADQLFTTLMGDAVEPRRAFIEENALK
                     AANIDI"
     gene            10783..11181
                     /gene="yidB"
                     /locus_tag="ACHOSC_RS01975"
                     /old_locus_tag="pgaptmp_000395"
     CDS             10783..11181
                     /gene="yidB"
                     /locus_tag="ACHOSC_RS01975"
                     /old_locus_tag="pgaptmp_000395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418153.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YidB family protein"
                     /protein_id="HGU4546408.1"
                     /translation="MGLFDEVVGAFLKGDAGKYQAILSWVEEQGGIQVLLEKLQSGGL
                     GAILSTWLSNQQGNQSVSGEQLESALGTNAVSDLGQKLGVDTSTASSLLAEQLPKIID
                     ALSPQGEVSPQANNDLLSAGMELLKGKLFR"
     gene            11296..12108
                     /gene="yidA"
                     /locus_tag="ACHOSC_RS01980"
                     /old_locus_tag="pgaptmp_000396"
     CDS             11296..12108
                     /gene="yidA"
                     /locus_tag="ACHOSC_RS01980"
                     /old_locus_tag="pgaptmp_000396"
                     /EC_number="3.1.3.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar-phosphatase"
                     /protein_id="HGU4546409.1"
                     /translation="MAIKLIAIDMDGTLLLPDHTISPAVKNAIAAARARGVNVVLTTG
                     RPYAGVHNYLKELHMELPGDYCITYNGALVQKAADGSTVAQTALSYDDYRFLEKLSRE
                     VGSHFHALDRTTLYTANRDISYYTVHESFVATIPLVFCEAEKMDPNTQFLKVMMIDEP
                     AILDQAIARIPQEVKEKYTVLKSAPYFLEILDKRVNKGTGVKSLADVLGIKPEEIMAI
                     GDQENDIAMIEYAGVGVAMDNAIPSVKEVANFVTKSNLEDGVAFAIEKYVLN"
     gene            complement(12154..12810)
                     /gene="yidX"
                     /locus_tag="ACHOSC_RS01985"
                     /old_locus_tag="pgaptmp_000397"
     CDS             complement(12154..12810)
                     /gene="yidX"
                     /locus_tag="ACHOSC_RS01985"
                     /old_locus_tag="pgaptmp_000397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YidX"
                     /protein_id="HGU4546410.1"
                     /translation="MKLNFKGFFKAAGLFPLALMLSGCISYALVSHTAKGSSGKYQSQ
                     SDTITGLSQAKDSNGTKGYVFVGESLDYLITDGADDIVKMLNDPALNRHNIQVADDAR
                     FVLNAGKKKFTGTISLYYHWNNEEEKALATHYGFACGVQHCTRSLENLKGTIHEKNKN
                     MDYSKVMAFYHPFKVRFYEYYSPRGIPDGVSAALLPVTVTLDIITAPLQFLVVYAVNQ
                     "
     gene            13088..13777
                     /gene="dgoR"
                     /locus_tag="ACHOSC_RS01990"
                     /old_locus_tag="pgaptmp_000398"
     CDS             13088..13777
                     /gene="dgoR"
                     /locus_tag="ACHOSC_RS01990"
                     /old_locus_tag="pgaptmp_000398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-galactonate utilization transcriptional
                     regulator DgoR"
                     /protein_id="HGU4546411.1"
                     /translation="MTLNKTDRIVITLGKQIVHGKYVPGSPLPAEAELCEEFATSRNI
                     IREVFRSLMAKRLIEMKRYRGAFVAPRNQWNYLDTDVLQWVLENDYDPRLISAMSEVR
                     NLVEPAIARWAAERATSSDLAQIESALNEMIANNQDREAFNEADIRYHEAVLQSVHNP
                     VLQQLSIAISSLQRAVFERTWMGDEANMPQTLQEHKALFDAIRHQDGDAAEQAALTMI
                     ASSTRRLKEIT"
     gene            13774..14652
                     /gene="dgoK"
                     /locus_tag="ACHOSC_RS01995"
                     /old_locus_tag="pgaptmp_000399"
     CDS             13774..14652
                     /gene="dgoK"
                     /locus_tag="ACHOSC_RS01995"
                     /old_locus_tag="pgaptmp_000399"
                     /EC_number="2.7.1.58"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydro-3-deoxygalactonokinase"
                     /protein_id="HGU4546412.1"
                     /translation="MTARYIAIDWGSTNLRAWLYQGDHCLESRQSEAGVTRLNGKSPA
                     AVLAEVTTDWREENTPVVMAGMVGSNVGWKVAPYLSVPARFSSIGEQLTSVGDNIWII
                     PGLCVSHDDNHNVMRGEETQLIGARTLAPSSLYVMPGTHCKWVQADSQQINDFRTVMT
                     GELHHLLLNHSLIGAGLPPQENSADAFAAGLERGLNAPAILPQLFEVRASHVLGTLPR
                     EQVSEFLSGLLIGAEVASMRDYVTHQHAITLVAGTSLTARYQQAFQAMGCDVTAVAGD
                     TAFQAGIRSIAHAVAN"
     gene            14636..15253
                     /gene="dgoA"
                     /locus_tag="ACHOSC_RS02000"
                     /old_locus_tag="pgaptmp_000400"
     CDS             14636..15253
                     /gene="dgoA"
                     /locus_tag="ACHOSC_RS02000"
                     /old_locus_tag="pgaptmp_000400"
                     /EC_number="4.1.2.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydro-3-deoxy-6-phosphogalactonate aldolase"
                     /protein_id="HGU4546413.1"
                     /translation="MQWQTKLPLIAILRGIKPDEALAHVGAVIDAGFDAVEIPLNSPQ
                     WEQSIPAIVDAYGDKALIGAGTVLKPEQVDALARMGCQLIVTPNIHSEVIRRAVGYGM
                     TVCPGCATATEAFTALEAGAQALKIFPSSAFGPQYIKALKAVLPSDIAVFAVGGVTPE
                     NLAQWIDAGCAGAGLGSDLYRAGQSVERTAQQAAAFVKAYREAVQ"
     gene            15250..16398
                     /gene="dgoD"
                     /locus_tag="ACHOSC_RS02005"
                     /old_locus_tag="pgaptmp_000401"
     CDS             15250..16398
                     /gene="dgoD"
                     /locus_tag="ACHOSC_RS02005"
                     /old_locus_tag="pgaptmp_000401"
                     /EC_number="4.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020077623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008869 - galactonate dehydratase activity
                     [Evidence IEA];
                     GO_process: GO:0009063 - amino acid catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="galactonate dehydratase"
                     /protein_id="HGU4546414.1"
                     /translation="MKITKITTYRLPPRWMFLKIETDEGVVGWGEPVIEGRARTVEAA
                     VHELGDYLIGQDPSRINDLWQVMYRAGFYRGGPILMSAIAGIDQALWDIKGKVLNAPV
                     WQLMGGLVRDKIKAYSWVGGDRPADVIDGIKTLREIGFDTFKLNGCEELGLIDNSRAV
                     DAAVNTVAQIREAFGNQIEFGLDFHGRVSAPMAKVLIKELEPYRPLFIEEPVLAEQAE
                     YYPKLAAQTHIPLAAGERMFSRFDFKRVLEAGGISILQPDLSHAGGITECYKIAGMAE
                     AYDVTLAPHCPLGPIALAACLHIDFVSYNAVLQEQSMGIHYNKGAELLDFVKNKEDFS
                     MVGGFFKPLTKPGLGVEIDEAKVIEFSKNAPDWRNPLWRHEDNSVAEW"
     gene            16473..17810
                     /gene="dgoT"
                     /locus_tag="ACHOSC_RS02010"
                     /old_locus_tag="pgaptmp_000402"
     CDS             16473..17810
                     /gene="dgoT"
                     /locus_tag="ACHOSC_RS02010"
                     /old_locus_tag="pgaptmp_000402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HGU4546415.1"
                     /translation="MVSGFAMPKIWRKLAMDIPVNAAKPGRRRYLTLVMIFITVVICY
                     VDRANLAVASAHIQEEFGITKAEMGYVFSAFAWLYTLCQIPGGWFLDRVGSRVTYFIA
                     IFGWSVATLFQGFATGLMSLIGLRAITGIFEAPAFPTNNRMVTSWFPEHERASAVGFY
                     TSGQFVGLAFLTPLLIWIQEMLSWHWVFIVTGGIGIIWSLIWFKVYQPPRLTKGISKA
                     ELDYIRDGGGLVDGDAPVKKEARQPLTAKDWKLVFHRKLIGVYLGQFAVASTLWFFLT
                     WFPNYLTQEKGITALKAGFMTTVPFLAAFVGVLLSGWVADLLVRKGFSLGFARKTPII
                     CGLLISTCIMGANYTNDPMMIMCLMALAFFGNGFASITWSLVSSLAPMRLIGLTGGVF
                     NFAGGLGGITVPLVVGYLAQGYGFAPALVYISAVALIGALSYILLVGDVKRVG"
     gene            complement(17807..18871)
                     /gene="cbrA"
                     /locus_tag="ACHOSC_RS02015"
                     /old_locus_tag="pgaptmp_000403"
     CDS             complement(17807..18871)
                     /gene="cbrA"
                     /locus_tag="ACHOSC_RS02015"
                     /old_locus_tag="pgaptmp_000403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418145.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin M resistance lipid reductase CbrA"
                     /protein_id="HGU4546416.1"
                     /translation="MEHFDVAIIGLGPAGSALARKLAGKMQVIALDKKHQCGTEGFSK
                     PCGGLLAPDAQRSFIRDGLTLPVDVIANPQIFSVKTVDVAASLTRNYQRSYININRHA
                     FDLWMKSLIPASVEIYHDSLCRKIWREDDKWHVIFRADGWEQHITARYLVGADGANSM
                     VRRHLYPDHQIRKYVAIQQWFAEKHPVPFYSCIFDNSITNCYSWSISKDGYFIFGGAY
                     PMKDGQTRFTTLKEKMSAFQFQFGKAVKSEKCTVLFPSRWQDFVCGKDNAFLIGEAAG
                     FISASSLEGISYALDSADILRSVLLKQPEKLNTAYWRATRKLRLKLFGKIVKSRCLTA
                     PALRKRIMRSGMAHIPQLKD"
     gene            18972..20186
                     /gene="yidR"
                     /locus_tag="ACHOSC_RS02020"
                     /old_locus_tag="pgaptmp_000404"
     CDS             18972..20186
                     /gene="yidR"
                     /locus_tag="ACHOSC_RS02020"
                     /old_locus_tag="pgaptmp_000404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312656.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3748 domain-containing protein"
                     /protein_id="HGU4546417.1"
                     /translation="MKQITFAPRNHLLTNTNTWTPDSQWLVFDVRPSGASFTGETIER
                     VNIHTGEVEVIYRASQGAHVGVVTVHPKSEKYVFIHGPENPDETWHYDFHHRRGVIAE
                     GGKVSNLDAMDITAPYMPGALRGGSHVHVFSPNGERVSFTYNDHVMHELDPALDLRNV
                     GVAAPFGPVNVQKQHPREYSGSHWCVLVSKTTPTPQPGSDEINRAYEEGWVGNHALAF
                     IGDTLSPKGEKVPELFIVELPQDEAGWKAAGDAPLSGTETTLPAPPRGVVQRRLTFTH
                     HRAYPGLVNVPRHWVRCNPQGTQIAFLMRDDNGIVQLWLISPQGGEPRQLTHNKTDIQ
                     SAFNWHPSGEWLGFVLDNRIACAHAQSGEVEYLTENHANPPSADAEVFSPDGQWLAWM
                     EGGQLWITETDR"
     gene            complement(20188..20520)
                     /gene="yidQ"
                     /locus_tag="ACHOSC_RS02025"
                     /old_locus_tag="pgaptmp_000405"
     CDS             complement(20188..20520)
                     /gene="yidQ"
                     /locus_tag="ACHOSC_RS02025"
                     /old_locus_tag="pgaptmp_000405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418143.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YceK/YidQ family lipoprotein"
                     /protein_id="HGU4546418.1"
                     /translation="MIRNVLLAFMICSGMTLLGGCSSVMSHTGGKEGTYPGTRASATM
                     IGDDETNWGTKSLAILDMPFTAVMDTLLLPWDVFRKDSSVRSRVEKSEANAQATNAVI
                     PPARMPDN"
     misc_feature    20760..20832
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01832"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="ROSE (Repression Of Heat Shock gene Expression)
                     occurs in the 5'-region of heat shock genes and acts as an
                     RNA thermometer to modulate expression; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF01832"
     gene            20826..21239
                     /gene="ibpA"
                     /locus_tag="ACHOSC_RS02030"
                     /old_locus_tag="pgaptmp_000406"
     CDS             20826..21239
                     /gene="ibpA"
                     /locus_tag="ACHOSC_RS02030"
                     /old_locus_tag="pgaptmp_000406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006687722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0006950 - response to stress [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="small heat shock chaperone IbpA"
                     /protein_id="HGU4546419.1"
                     /translation="MRNFDLSPLYRSAIGFDRLFNHLENNQSQSNGGYPPYNVELVDE
                     NHYRIAIAVAGFAESELEITAQDNLLVVKGAHADEQKERTYLYQGIAERNFERKFQLA
                     ENIHVRGANLVNGLLYIDLERVIPEAKKPRRIEIN"
     gene            21351..21779
                     /gene="ibpB"
                     /locus_tag="ACHOSC_RS02035"
                     /old_locus_tag="pgaptmp_000407"
     CDS             21351..21779
                     /gene="ibpB"
                     /locus_tag="ACHOSC_RS02035"
                     /old_locus_tag="pgaptmp_000407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709512.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0006950 - response to stress [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="small heat shock chaperone IbpB"
                     /protein_id="HGU4546420.1"
                     /translation="MRNFDLSPLMRQWIGFDKLANALQNAGESQSFPPYNIEKSDDNH
                     YRITLALAGFRQEDLEIQLEGTRLSVKGTPEQPKEEKKWLHQGLMNQPFSLSFTLAEN
                     MEVSGATFVNGLLHIDLIRNEPEPIAAQRIAISERPALNS"
     gene            21976..23637
                     /gene="yidE"
                     /locus_tag="ACHOSC_RS02040"
                     /old_locus_tag="pgaptmp_000408"
     CDS             21976..23637
                     /gene="yidE"
                     /locus_tag="ACHOSC_RS02040"
                     /old_locus_tag="pgaptmp_000408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312652.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative transporter"
                     /protein_id="HGU4546421.1"
                     /translation="MSDIALTVSILALVAVVGLFIGNVKFRGIGLGIGGVLFGGIIVG
                     HFVSQAGMTLSSDMLHVIQEFGLILFVYTIGIQVGPGFFASLRVSGLRLNLFAVLIVI
                     IGGLVTAILHKLFDIPLPVVLGIFSGAVTNTPALGAGQQILRDLGTPMEMVDQMGMSY
                     AMAYPFGICGILFTMWMLRVIFRVNVETEAQQHESSRTNGGALIKTINIRVENPNLHD
                     LAIKDVPILNGDKIICSRLKREETLKVPSPDTIIQLGDLLHLVGQPADLHNAQLVIGQ
                     EVDTSLSTKGTDLRVERVVVTNENVLGKRIRDLHFKERYDVVISRLNRAGVELVASGD
                     ISLQFGDILNLVGRPSAIDAVANVLGNAQQKLQQVQMLPVFIGIGLGVLLGSIPVFVP
                     GFPAALKLGLAGGPLIMALILGRIGSIGKLYWFMPPSANLALRELGIVLFLSVVGLKS
                     GGDFVNTLVNGEGLSWIGYGALITAVPLITVGILARMLAKMNYLTMCGMLAGSMTDPP
                     ALAFANNLHPTSGAAALSYATVYPLVMFLRIITPQLLAVLFWSIG"
     gene            complement(23634..24350)
                     /gene="yidP"
                     /locus_tag="ACHOSC_RS02045"
                     /old_locus_tag="pgaptmp_000409"
     CDS             complement(23634..24350)
                     /gene="yidP"
                     /locus_tag="ACHOSC_RS02045"
                     /old_locus_tag="pgaptmp_000409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HGU4546422.1"
                     /translation="MIYKSIAERLRIRLNSADFTLNSLLPGEKKLAEEFAVSRMTIRK
                     AIDLLVAWGLVVRRHGSGTYLVRKDVLHQTASLTGLVEVLKRQGKTVTSQVLIFEIMP
                     APPAIASQLRIQINEQIYFSRRVRFVEGKPLMLEDSYMPVKLFRNLSLQHLEGSKFEY
                     IEQECGILIGGNYESLTPVLADRLLARQMKVAEHTPLLRITSLSYSESGEFLNYSVMF
                     RNASEYQVEYHLRRLHPEKS"
     gene            24646..26262
                     /locus_tag="ACHOSC_RS02050"
                     /old_locus_tag="pgaptmp_000410"
     CDS             24646..26262
                     /locus_tag="ACHOSC_RS02050"
                     /old_locus_tag="pgaptmp_000410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312650.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HGU4546423.1"
                     /translation="MLSQIQRFGGAMFTPVLLFPFAGIVVGLAILLQNPMFVGESLTD
                     PNSLFAQIVHIIEEGGWTVFRNMPLIFAVGLPIGLAKQAQGRACLAVMVSFLTWNYFI
                     NAMGMTWGSYFGVDFTQDAVAGSGLTMMAGIKTLDTSIIGAIIISGIVTALHNRLFDK
                     KLPVFLGIFQGTSYVVIIAFLVMIPCAWLTLLGWPKVQMGIESLQAFLRSAGALGVWV
                     YTFLERILIPTGLHHFIYGPFIFGPAAVEGGIQMYWAQHLQEFSLSAEPLKSLFPEGG
                     FALHGNSKIFGAVGISLAMYFTAAPENRVKVAGLLIPATLTAMLVGITEPLEFTFLFI
                     SPLLFAVHAVLAASMSTVMYLFGVVGNMGGGLIDQVLPQNWIPMFSNHADMMLTQIAI
                     GLCFTLLYFVVFRTLILQFNMCTPGREDAEVKLYSKAEYKASRGQTTAAEPKKELDQA
                     AGILQALGGVGNISSINNCATRLRIALHDMSQTLDDEVFKKLGAHGVFRSGDAIQVII
                     GLHVSQLREQLDSLINSHQSAENVAITEAV"
     gene            26262..27076
                     /locus_tag="ACHOSC_RS02055"
                     /old_locus_tag="pgaptmp_000411"
                     /pseudo
     CDS             26262..27076
                     /locus_tag="ACHOSC_RS02055"
                     /old_locus_tag="pgaptmp_000411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312649.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(27073..27966)
                     /gene="yidL"
                     /locus_tag="ACHOSC_RS02060"
                     /old_locus_tag="pgaptmp_000412"
     CDS             complement(27073..27966)
                     /gene="yidL"
                     /locus_tag="ACHOSC_RS02060"
                     /old_locus_tag="pgaptmp_000412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418136.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HGU4546424.1"
                     /translation="MNGKLQSSDVKNETPYNIPLLINENVISSGISLISLWHTYADEH
                     YRVIWPRDKKKPLIANSWVAVYTVQGCGKILLKNGEQITLHGNCIIFLKPMDIHSYHC
                     EGLVWEQYWMEFTPTSMMDIPVGQQSVIYNGEIYNQELTEVAELITSPEAIKNNLAVA
                     FLTKIIYQWICLMYADGKKDPQRRQIEKLIATLHASLQQRWSVADMAATIPCSEAWLR
                     RLFLRYTGKTPKEYYLDARLDLALSLLKQQGNSVGEVADTLNFFDSFHFSKAFKHKFG
                     YAPSAVLKNTDQHPTDASPHN"
     gene            28133..29848
                     /gene="yidK"
                     /locus_tag="ACHOSC_RS02065"
                     /old_locus_tag="pgaptmp_000413"
     CDS             28133..29848
                     /gene="yidK"
                     /locus_tag="ACHOSC_RS02065"
                     /old_locus_tag="pgaptmp_000413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="solute:sodium symporter family transporter"
                     /protein_id="HGU4546425.1"
                     /translation="MNSLQILSFVGFTLLVAVITWWKVRKTDTGSQQGYFLAGRSLKA
                     PVIAASLMLTNLSTEQLVGLSGQAYKSGMSVMGWEVTSAVTLIFLALIFLPRYLKRGI
                     ATIPDFLEERYDKTTRIIIDFCFLIATGVCFLPIVLYSGALALNSLFHVGESLQISHG
                     AAIWLLVILLGLAGILYAVIGGLRAMAVADSINGIGLVIGGLMVPVFGLIAMGKGSFM
                     QGIEQLTTVHAEKLNSIGGPTDPLPIGAAFTGLILVNTFYWCTNQGIVQRTLASKSLA
                     EGQKGALLTAVLKMLDPLVLVLPGLIAFHLYQDLPKADMAYPTLVNNVLPVPMVGFFG
                     AVLFGAVISTFNGFLNSASTLFSMGIYRRIINQNAEPQQLVTVGRKFGFFIAIVSVLV
                     APWIANAPQGLYSWMKQLNGIYNVPLVTIIIMGFFFPRIPALAAKVAMGIGIISYITI
                     NYLVKFDFHFLYVLACTFCINVVVMLVIGFIKPRATPFTFKDAFAVDMKPWRNVKIAS
                     IGILFAMIGVYAGLAEFGGYGTRWLAMISYFIAAVVIVYLIFDSWRHRHDPAVTFTPD
                     AKDSL"
     gene            29845..31338
                     /gene="yidJ"
                     /locus_tag="ACHOSC_RS02070"
                     /old_locus_tag="pgaptmp_000414"
     CDS             29845..31338
                     /gene="yidJ"
                     /locus_tag="ACHOSC_RS02070"
                     /old_locus_tag="pgaptmp_000414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase-like hydrolase/transferase"
                     /protein_id="HGU4546426.1"
                     /translation="MKRPNFLFIMTDTQATNMVGCYSGKPLNTQNIDSLAAEGIRFNS
                     AYTCSPVCTPARAGLFTGIYANQSGPWTNNVAPGKNISTMGRYFKDAGYHTCYIGKWH
                     LDGHDYFGTGECPPEWDADYWFDGANYLSELTEKEISLWRNGLNSVEDLQANHIDETF
                     TWAHRISNRAVDFLQQPARAEEPFLMVVSYDEPHHPFTCPVEYLEKYADFYYDLGEKA
                     QDGLANKPEHHRLWAQAMPSPVGDDGLYHHPLYFACNDFVDDQIGRVINALTPEQREN
                     TWVIYTSDHGEMMGAHKLISKGAAMYDDITRIPLIIRSPQGERRQVDTPVSHIDLLPT
                     MMALADIEKPEILPGENILAVKEPRGVMVEFNRYEIEHDSFGGFIPVRCWVTDDFKLV
                     LNLFTSDELYDRRNDPNEMHNLIDDIRFADVRSKMHDALLDYMDKIRDPFRSYQWSLR
                     PWRKDALPRWMGAFRPRPQDGYSPVVRDYDTGLPTQGVKVEEKKQKF"
     gene            complement(31385..31834)
                     /gene="yidI"
                     /locus_tag="ACHOSC_RS02075"
                     /old_locus_tag="pgaptmp_000415"
     CDS             complement(31385..31834)
                     /gene="yidI"
                     /locus_tag="ACHOSC_RS02075"
                     /old_locus_tag="pgaptmp_000415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YidI"
                     /protein_id="HGU4546427.1"
                     /translation="MGIIAQNKISSLGMLFGAIALMMGIIHFSFGPFSAPPPTFESIV
                     ADKTAEIKRGLLAGIKGEKITTVEKKEDVDVDKILDQSGIALAIAALLCAFIGGMRKE
                     NRWGIRGALVFGGGTLAFHTLLFGIGIVCSILLIFLIFSFLTGGSLV"
     gene            31944..32291
                     /gene="yidH"
                     /locus_tag="ACHOSC_RS02080"
                     /old_locus_tag="pgaptmp_000416"
     CDS             31944..32291
                     /gene="yidH"
                     /locus_tag="ACHOSC_RS02080"
                     /old_locus_tag="pgaptmp_000416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YidH family protein"
                     /protein_id="HGU4546428.1"
                     /translation="MKISRLGEAPDYRFSLANERTFLAWIRTALGFLAAGVGLDQLAP
                     DFATPVIRELLALLLCLFSGGLAMYGYLRWLRNEKAMRLKEDLPYTNSLLIISLILMV
                     VAVIVMGLVLYAG"
     gene            32281..32643
                     /gene="yidG"
                     /locus_tag="ACHOSC_RS02085"
                     /old_locus_tag="pgaptmp_000417"
     CDS             32281..32643
                     /gene="yidG"
                     /locus_tag="ACHOSC_RS02085"
                     /old_locus_tag="pgaptmp_000417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF202 domain-containing protein"
                     /protein_id="HGU4546429.1"
                     /translation="MPDSRKARRIADPGLQPERTSLAWFRTMLGYGALMALAIKHNWH
                     QAGMLFWISIGILAIVALILWHYTRNRNLMDVTNSDFSQFHVVRDKFLISLAVLSLAI
                     LFAVTHIHQLIVFIERVA"
     gene            32640..33137
                     /gene="yidF"
                     /locus_tag="ACHOSC_RS02090"
                     /old_locus_tag="pgaptmp_000418"
     CDS             32640..33137
                     /gene="yidF"
                     /locus_tag="ACHOSC_RS02090"
                     /old_locus_tag="pgaptmp_000418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HGU4546430.1"
                     /translation="MTGSQVIDAEEDRHKLVVEYKDALQPADFYHNFKQRGIRSVQLI
                     PYLEFDDRGDLTAASVTAELWGKFLIALFECWVRADISRISIELFDATLQKWCGSENP
                     QPRRDCQACDWHRLCPHARQETPDSVLCAGYQAFYSYSAPHMRVMRDLIKQHRSPMEL
                     MTMLR"
     gene            complement(33145..34329)
                     /gene="emrD"
                     /locus_tag="ACHOSC_RS02095"
                     /old_locus_tag="pgaptmp_000419"
     CDS             complement(33145..34329)
                     /gene="emrD"
                     /locus_tag="ACHOSC_RS02095"
                     /old_locus_tag="pgaptmp_000419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418129.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter EmrD"
                     /protein_id="HGU4546431.1"
                     /translation="MKRQRNVNLLLMLVLLVAVGQMAQTIYIPAIADMARDLNVREGA
                     VQSVMGAYLLTYGVSQLFYGPISDRVGRRPVILVGMSIFMLATLVAVTTSSLTVLIAA
                     SAMQGMGTGVGGVMARTLPRDLYERTQLRHANSLLNMGILVSPLLAPLIGGLLDTMWN
                     WRACYLFLLVLCAGVTFSMARWMPETRPVDAPRTRLLTSYKTLFGNSGFNCYLLMLIG
                     GLAGIAAFEACSGVLMGAVLGLSSMTVSILFILPIPAAFFGAWFAGRPNKRFSTLMWQ
                     SVICCLLAGLLMWIPDWFGVMNVWTLLVPAALFFFGAGMLFPLATSGAMEPFPFLAGT
                     AGALVGGLQNIGSGVLASLSAMLPQTGQGSLGLLMTLMGLLIVLCWLPLATRMSHQGQ
                     PV"
     gene            34411..34485
                     /gene="ysdE"
                     /locus_tag="ACHOSC_RS02100"
                     /old_locus_tag="pgaptmp_000420"
     CDS             34411..34485
                     /gene="ysdE"
                     /locus_tag="ACHOSC_RS02100"
                     /old_locus_tag="pgaptmp_000420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YsdE"
                     /protein_id="HGU4546432.1"
                     /translation="MNVSQIYARNGELFSGRICKQKRQ"
     gene            complement(34748..34837)
                     /gene="tisB"
                     /locus_tag="ACHOSC_RS02105"
                     /old_locus_tag="pgaptmp_000421"
     CDS             complement(34748..34837)
                     /gene="tisB"
                     /locus_tag="ACHOSC_RS02105"
                     /old_locus_tag="pgaptmp_000421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin TisB"
                     /protein_id="HGU4546433.1"
                     /translation="MSLVDIAILILKLIVAALQLLDAVLKYLK"
     gene            35402..35500
                     /gene="ivbL"
                     /locus_tag="ACHOSC_RS02110"
                     /old_locus_tag="pgaptmp_000422"
     CDS             35402..35500
                     /gene="ivbL"
                     /locus_tag="ACHOSC_RS02110"
                     /old_locus_tag="pgaptmp_000422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ilvB operon leader peptide IvbL"
                     /protein_id="HGU4546434.1"
                     /translation="MTTSMFNAKLLPTALSAAVVVVRVVVVVGNAP"
     gene            35606..37294
                     /gene="ilvB"
                     /locus_tag="ACHOSC_RS02115"
                     /old_locus_tag="pgaptmp_000423"
     CDS             35606..37294
                     /gene="ilvB"
                     /locus_tag="ACHOSC_RS02115"
                     /old_locus_tag="pgaptmp_000423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0003984 - acetolactate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0009082 - branched-chain amino acid
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase large subunit"
                     /protein_id="HGU4546435.1"
                     /translation="MASSGTTSTRKRFTGAEFIVHFLEQQGIKIVTGIPGGSILPVYD
                     ALSQSTQIRHILARHEQGAGFIAQGMARTDGKPAVCMACSGPGATNLVTAIADARLDS
                     IPLICITGQVPASMIGTDVFQEVDTYGISIPITKHNYLVRHIEELPQVMSDAFRIAQS
                     GRPGPVWIDIPKDVQTAVFEIETQPAMAEKAAAPAFSEESIRDAAAMINAAKRPVLYL
                     GGGVINAPARVRELAEKAQLPTTMTLMALGMLPKAHPLSLGMLGMHGVRSTNYILQEA
                     DLLIVLGARFDDRAIGKTEQFCPNAKIIHVDIDRAELGKIKQPHVAIQADVDDVLAQL
                     IPQVEAQPRAEWHQLVADLQREFPCPIPKACDPLSHYGLINAVAACVDDNAIITTDVG
                     QHQMWTAQAYPLNRPRQWLTSGGLGTMGFGLPAAIGAALANPDRKVLCFSGDGSLMMN
                     IQEMATASENQLDVKIILMNNEALGLVHQQQSLFYEQGVFAATYPGKINFMQIAAGFG
                     LETCDLNNEADPQAALQEIINRPGPALIHVRIDAEEKVYPMVPPGAANTEMVGE"
     gene            37298..37588
                     /gene="ilvN"
                     /locus_tag="ACHOSC_RS02120"
                     /old_locus_tag="pgaptmp_000424"
     CDS             37298..37588
                     /gene="ilvN"
                     /locus_tag="ACHOSC_RS02120"
                     /old_locus_tag="pgaptmp_000424"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase small subunit"
                     /protein_id="HGU4546436.1"
                     /translation="MQNTTHDNVILELTVRNHPGVMTHVCGLFARRAFNVEGILCLPI
                     QDSDKSHIWLLVNDDQRLEQMISQIDKLEDVVKVQRNQSDPTMFNKIAVFFQ"
     gene            37663..38253
                     /gene="uhpA"
                     /locus_tag="ACHOSC_RS02125"
                     /old_locus_tag="pgaptmp_000425"
     CDS             37663..38253
                     /gene="uhpA"
                     /locus_tag="ACHOSC_RS02125"
                     /old_locus_tag="pgaptmp_000425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator UhpA"
                     /protein_id="HGU4546437.1"
                     /translation="MITVALIDDHLIVRSGFAQLLGLEPDLQVVAEFGSGREALAGLP
                     GRGVQVCICDISMPDISGLELLSQLPKGMATIMLSVHDSPALVEQALNAGARGFLSKR
                     CSPDELIAAVHTVATGGCYLTPDIAIKLASGRQDPLTKRERQVAEKLAQGMAVKEIAA
                     ELGLSPKTVHVHRANLMEKLGVSNDVELARRMFDGW"
     gene            38253..39755
                     /gene="uhpB"
                     /locus_tag="ACHOSC_RS02130"
                     /old_locus_tag="pgaptmp_000426"
     CDS             38253..39755
                     /gene="uhpB"
                     /locus_tag="ACHOSC_RS02130"
                     /old_locus_tag="pgaptmp_000426"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418124.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal transduction histidine-protein
                     kinase/phosphatase UhpB"
                     /protein_id="HGU4546438.1"
                     /translation="MKTLFSRLITVIACFFIFSAAWFCLWSISLHLVERPDMAVLLFP
                     FGLRLGLMLQCPRGYWPVLLGAEWLLIYWLTQAVGLTHFPLLMIGSLLTLLPVALISR
                     YRHQRDWRTLLLQGAALTAAALLQSLPWLWHGKESWNALLLTLTGGLTLAPICLVFWH
                     YLANNTWLPLGPSLVSQPINWRGRHLVWYLLLFVISLWLQLGLPDELSRFTPFCLALP
                     IIALAWHYGWQGALIATLMNAIALIASQTWRDHPVDLLLSLLVQSLTGLLLGAGIQRL
                     RELNQSLQKELARNQHLAERLLETEESVRRDVARELHDDIGQTITAIRTQAGIVQRLA
                     ADNASVKQSGQLIEQLSLGVYDAVRRLLGRLRPRQLDDLTLEQAIRSLMREMELEGRG
                     IVSHLEWRIDESALSENQRVTLFRVCQEGLNNIVKHADASAVTLQGWQQDERLMLVIE
                     DDGSGLPPGSGQQGFGLTGMRERVTALGGTLHISCLHGTRVSVSLPQRYV"
     gene            39765..41084
                     /gene="uhpC"
                     /locus_tag="ACHOSC_RS02135"
                     /old_locus_tag="pgaptmp_000427"
     CDS             39765..41084
                     /gene="uhpC"
                     /locus_tag="ACHOSC_RS02135"
                     /old_locus_tag="pgaptmp_000427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709527.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter family glucose-6-phosphate
                     receptor UhpC"
                     /protein_id="HGU4546439.1"
                     /translation="MLPFLKAPADAPLMTDKYEIDARYRYWRRHILLTIWLGYALFYF
                     TRKSFNAAVPEILANGVLSRSDIGLLATLFYITYGVSKFVSGIVSDRSNARYFMGIGL
                     IATGIMNILFGFSTSLWAFAVLWVLNAFFQGWGSPVCARLLTAWYSRTERGGWWALWN
                     TAHNVGGALIPIVMAASALHYGWRAGMMIAGCMAIVVGIFLCWRLRDRPQALGLPAVG
                     EWRHDALEIAQQQEGAGLTRKEILTKYVLLNPYIWLLSFCYVLVYVVRAAINDWGNLY
                     MSETLGVDLVTANTAVTMFELGGFIGALVAGWGSDKLFNGNRGPMNLIFAAGILLSVG
                     SLWLMPFASYVMQATCFFTIGFFVFGPQMLIGMAAAECSHKEAAGAATGFVGLFAYLG
                     ASLAGWPLAKVLDTWHWSGFFVVISIAAGVSALLLLPFLNAQAPREA"
     gene            41340..42731
                     /gene="uhpT"
                     /locus_tag="ACHOSC_RS02140"
                     /old_locus_tag="pgaptmp_000428"
     CDS             41340..42731
                     /gene="uhpT"
                     /locus_tag="ACHOSC_RS02140"
                     /old_locus_tag="pgaptmp_000428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hexose-6-phosphate:phosphate antiporter"
                     /protein_id="HGU4546440.1"
                     /translation="MLAFLNQVRKPTLDLPLEVRRKMWFKPFMQSYLVVFIGYLTMYL
                     IRKNFNIAQNDMISTYGLSMTQLGMIGLGFSITYGVGKTLVSYYADGKNTKQFLPFML
                     ILSAICMLGFSASMGSGSVSLFLMIAFYALSGFFQSTGGSCSYSTITKWTPRRKRGTF
                     LGFWNISHNLGGAGAAGVALFGANYLFDGHVIGMFIFPSIIALIVGFIGLRYGSDSPE
                     SYGLGKAEELFGEEISEEDKETESTDMTKWQIFVEYVLKNKVIWLLCFANIFLYVVRI
                     GIDQWSTVYAFQELKLSKAVAIQGFTLFEAGALVGTLLWGWLSDLANGRRGLVACIAL
                     ALIIATLGVYQHASNEYIYLASLFALGFLVFGPQLLIGVAAVGFVPKKAIGAADGIKG
                     TFAYLIGDSFAKLGLGMIADGTPVFGLTGWAGTFAALDIAAIGCICLMAIVAVMEERK
                     IRREKKIQQLTVA"
     gene            complement(42776..>44485)
                     /gene="adeD"
                     /locus_tag="ACHOSC_RS02145"
                     /old_locus_tag="pgaptmp_000429"
     CDS             complement(42776..>44485)
                     /gene="adeD"
                     /locus_tag="ACHOSC_RS02145"
                     /old_locus_tag="pgaptmp_000429"
                     /EC_number="3.5.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenine deaminase"
                     /protein_id="HGU4546441.1"
                     /translation="LLAVSRGDAVADYIIDNVSILDLINGGEISGPIVIKGRYIAGVG
                     AEYADAPALQRIDARGATAVPGFIDAHLHIESSMMTPVTFETATLPRGLTTVICDPHE
                     IVNVMGEAGFAWFARCAEQARQNQYLQVSSCVPALEGCDVNGASFTLEQMLAWRDHPQ
                     VTGLAEMMDYPGVISGQNALLDKLDAFRHLTLDGHCPGLGGKELNAYITAGIENCHES
                     YQLEEGRRKLQLGMSLMIREGSAARNLNALAPLINEFNSPQCMLCTDDRNPWEIAHEG
                     HIDALIRRLIEQHNVPLHVAYRVASWSTARHFGLNHLGLLAPGKQADIVLLSDARKVT
                     VQQVLVKGEPIDAQTLQAEESARLAQSAPPYGNTIARQPVSASDFALQFTPGKRYRVI
                     DVIHNELITHSHSSVYSENGFDRDDVSFIAVLERYGQRLAPACGLLGGFGLNEGALAA
                     TVSHDSHNIVVIGRSAEEMALAVNQVIQDGGGLCVVRNGQVQSHLPLPIAGLMSTDTA
                     QSLAEQIDALKAAARECGPLPDEPFIQMAFLSLPVIPALKLTSQGLFDGEKFAFTTLE
                     VTE"
BASE COUNT        10237 a        11445 c        12095 g        10708 t
ORIGIN      
        1 ctaacaatga agcgaagccg catctgacag tcagaatacg gcttcgtaag cgcggttact
       61 taccaataca gaagctggag aaaatccgcc ccagcaggtc gtctgaagta aattccccgg
      121 tgatttcgct taagttctgc tgtgccaggc gcaactcttc cgccagcagt tcacccgccc
      181 aggcacctaa cagttgcgct ttaccttgct gcaaatgctc cgctgcctgt tccagcgcct
      241 gtaagtggcg acggcgcgcc aggaaaccgc cttccatgtt ggtgtcaaag cccatgctct
      301 gtttgagatg gttacgcagc acgtccacgc cttcaccagt ccttgccgag agacgaatta
      361 acgcgtgacc gttcacttca ctcattccca gcgtttcgcc ggtgatatcg gctttattgc
      421 gcaccacggt gatcggcagt ttcgctggca gacgggcgat aaattccggc cagatctctg
      481 ccggatccac ggcgtctgtt gtggtgccat cgaccataaa cagcacccgg tcggcctgtt
      541 caatttcctg ccacgcgcgc tcgatcccaa tacgttccac ttcgtcactg gcttcacgta
      601 gcccggcggt gtcgatgata tgcagcggca ttccgtcaat gtggatatgc tcacgcaaca
      661 cgtcacgcgt agttccggcg atatcggtta cgattgctgc ttcacggcca gccagcgcgt
      721 ttaacaggct cgatttaccg gcgttaggac gtccggcaat caccactttc atcccttcgc
      781 gcaacaaact accctgacgt gcttcagcac gcactgcatc aagatcggca ataacgtcat
      841 tgagctgggc ttcaattttg ccgtcggaga ggaaatcgat ctcttcatca gggaaatcaa
      901 ttgccgcttc gacgtaaatg cgcaagtggg tgagggcttc taccagatga ttaacccgtg
      961 cggagaatgc gccttgcagc gagttaagtg ccgaacgggc cgcctgttcc gagctggcgt
     1021 cgataagatc ggcaatcgcc tcagcctggg ctaagtcgag tttatcgtaa agaaacgcgc
     1081 gttcggaaaa ctcaccaggg cgagcaatcc gcaggccggg aatggtcaga atgcgtttta
     1141 acagcaggtc gaggatcacc ggaccgccat gaccctgcag ttcaagcaca tcttcgccgg
     1201 tgaacgagtt cgggccaggg aaccatagcg caatcccctg atcgagcacg ctgccgtcgg
     1261 cgtctttaaa tggcaaataa tcggcgtagc gcggcttagg tagtttaccc agcacggttt
     1321 cggcaacttc acgggctttg aggccggaga tgcgcaggat gccaacaccg ccacgtcccg
     1381 gaggcgtggc ctgggctacg atagtgtcat tatcgctcat gatgttcctg ttgctttgtg
     1441 tggcggatgc gcggtgctta tccgccctac gaaaagaaaa aaggcggtca actgaccgcc
     1501 cttattttag cgaaaactca ccgaatcagg attttttctt ctcgcggcta tgcaggccac
     1561 gtttttccag accacggtaa atcagctgct gctgaataat ggttaccagg ttgctgacga
     1621 tatagtacag caccagacct gacgggaacc acaggaagaa cacggtgaag atgaccggca
     1681 taaaggtcat gatcttctgc tgcatcgggt cggtcactgt ggtcggcgac atcttctgaa
     1741 tgaagaacat cgttacgccc atcaggatcg gcaggatgta gtacgggtcc tgtgccgaca
     1801 ggtcgtggat ccacagtgca aacggtgcct gacgcagttc aacggaaccc atcagcatgt
     1861 agtacaacgc caggaagatt ggcatctgga tcagcagcgg gaagcagccg cccagcgggt
     1921 taaccttctc agctttgtac agcgccatca tttcctggct gatacgctgt ttgtcatcgc
     1981 ccagacgctc acgcattgcc tgaatcttcg gctgcagcat acgcatcttc gccatggagg
     2041 tgtactgcgc tttggtcagc gggtacatga tgccacgaac gataaaggtg atgatgataa
     2101 tggagaagcc ccagttaccc acaaagctat ggatccattt cagcagtttg aacagcggct
     2161 gagagatgaa ccacaaccaa ccgtaatcaa cggtcagatc caggtgcgga gcaacagctg
     2221 ccattttgtc ctggatttcc gggccaaccc acagggtgct gttcatcgcg ccagtctgac
     2281 caggctgaac cagtaccggc tgagatttat agccgatagc ggcgatgccg ttacccagat
     2341 tagcggtata gaagttgttg gtaccgtcgt tatgcgggat ccacgccgtc gcgaaatact
     2401 gttgcagcat cgccacccaa ccacctttcg aagagatgtt caggttttcg ttatcggcaa
     2461 tggtatcgaa cttgtatttc tcatacttct cgtcaggcgt ggagtacgcc gcgccacgga
     2521 aggtgtgcag tgcgaagttg ctgcttccgg tatcgagatg cggtggcaga gtgatggatt
     2581 gcttcaactg accaaaggtg gagatttcca gcggtttctc gccagcgttc tgcacgttgt
     2641 agttgacgtt gacagcgtaa tcaccacgtt tcaggacaaa cgttttggta aacgtgttgc
     2701 ctgccgcgtc ggtatacgtc atcggcacct gcagttcgtt ttgaccttca gccagcacat
     2761 aagcgtcttt ttcaacgtta tacagcggac gcgggccgtt agccgggtta tccgggccat
     2821 cacgaccggt cagaccgctc tgtgcctgat aaataaactg cggtgaagtt tccagcagct
     2881 ggaacggctg ggtagagttc agctctttcg ggtaagcagg cagcagagct tgctcaacat
     2941 caccaccacg ggtgttgatg gtcagatcaa gcacgtcggt cttaaccgag atcagtttcc
     3001 cctggccact ggccggtacg ccctggtcgg cggcgctacc cgctgcggtg gtcgttgtct
     3061 gcgtggtctg ttgggcctga ggttgcgggt ttttatcctg ctcccaggct tgccagatca
     3121 tgaaagacac gaacagcaaa gcgatgacta aaagattgcg ttgcgaatcc atcgttagtg
     3181 ttctctggta tcaaatggtc cgggcgggac gggatcgtca ccaccagggt gtaaagggtg
     3241 gcattttaat acgcgtttca ccgtcaacca actgcctttt atcactccaa acctgcgcaa
     3301 tgcctcaatt ccgtagcttg aacaggttgg agtgaaacga caatgcggcc cgagtagcgg
     3361 actaatcagg cgttgataga cccgaatgag ggctatcagg acccgcgagc caggcgacag
     3421 tggcggcgcc ataatttttc caacgcttcc gagagagcac ggttatcgag gtcggcaacc
     3481 ccttttttcg ccaccaccac gaaatccata gccgggagtt catgttggcg cagacggaag
     3541 ctttcacgcg tcagacgttt aatccgattg cgttcatggg cgcgtcgaac gtttttcttg
     3601 gcgactgtaa gaccgatacg gggatgcccc agcgaattca ggcggccgag aatggtaatt
     3661 tgcggcgtgc cagcccgttg tggctgctgg aagacgaatg tgaattgact gggagttaac
     3721 aagcgtaact ccctgggaaa tgcgagctta accactcagg ggttagcttt attacttaga
     3781 aacggtcaga cgagcgcggc ctttagcacg acgacgtgcc agaacctgac gaccattttt
     3841 agtagccata cgagcacgga agccgtgaga acggttgcgc ttcagtacag acggttgaaa
     3901 agtgcgtttc atggcgattt ctacctaaac ttgaataaat tcaatggctt tattggatat
     3961 ccgccgaaaa atgaaacgat ggacaccgaa gccatgggtg attaaagagg ccggattgta
     4021 ataattgtac actccggagt caattctctt tccttattta ccgcgctttt ccgcaccttt
     4081 tcgcagggaa aatgtacgac ctcacaccag tgaaaaccag catggcgcgc cgggtggagg
     4141 attatacggg ctgatgggta aagcgcaagg atcgtcctgg atctttatta gatcgattaa
     4201 gccaattttt gtctatggtc attaaatttt ccaatatgcg gcgtaaatcg tgcccgcctc
     4261 gcggcaggat cgtttacact tagcgaggtc tggaaagtcc tgtggataaa tcgggaaaat
     4321 ctgtgagaaa cagaagatct cttgcgcagt ttaggctatg atccgcggtc ccgatcgttt
     4381 tgcaggatct tgatcgggca tataaccgca gacagcggtt cgtgcgtcac cctcaagcag
     4441 ggtcttttcg acgtacgtca acaatcatga atgtttcagc cttagtcatt atcgactttt
     4501 gttcgagtgg agtccgccgt gtcactttcg ctttggcagc agtgtcttgc ccgattgcag
     4561 gatgagttac cagccacaga attcagtatg tggatacgcc cattgcaggc ggaactgagc
     4621 gataacacgc tggccctgta cgcgccaaac cgttttgtcc tcgattgggt acgggacaag
     4681 taccttaata atatcaatgg actgctaacc agtttctgcg gagcggatgc cccacagctg
     4741 cgttttgaag tcggcaccaa accggtgacg caaacgccac aagcggcagt gacgagcaac
     4801 gtcgcggccc ctgcacaggt ggcgcaaacg cagccgcaac gtgctgcgcc ttctacgcgc
     4861 tcaggttggg ataacatccc ggccccggca gaaccgacct atcgttctaa cgtaaacgtc
     4921 aaacacacgt ttgataactt cgttgaaggt aaatctaacc aactggcgcg cgcggcggct
     4981 cgccaggtgg cggataaccc tggcggtgcc tataacccgt tgttccttta tggcggcacg
     5041 ggtctgggta aaactcacct gctgcatgcg gtgggtaacg gcattatggc gcgcaagccg
     5101 aatgccaaag tggtttatat gcactccgag cgctttgttc aggacatggt taaagccctg
     5161 caaaacaacg cgatcgaaga gtttaaacgc tactaccgtt ccgtagatgc actgctgatc
     5221 gacgatattc agttttttgc taataaagaa cgatctcagg aagagttttt ccataccttc
     5281 aacgccctgc tggaaggtaa tcaacagatc attctcacct cggatcgcta tccgaaagag
     5341 atcaacggcg ttgaggatcg tttgaaatcc cgcttcggtt ggggactgac tgtggcaatc
     5401 gaaccgccag agctggaaac ccgtgtggcg atcctgatga aaaaggccga cgaaaacgac
     5461 attcgtttgc cgggcgaagt ggcgttcttt atcgccaagc gtctacgatc taacgtacgt
     5521 gagctggaag gggcgctgaa ccgcgtcatt gccaatgcca actttaccgg acgggcgatc
     5581 accatcgact tcgtgcgtga ggcgctgcgc gacttgctgg cattgcagga aaaactggtc
     5641 accatcgaca atattcagaa gacggtggcg gagtactaca agatcaaagt cgcggatctc
     5701 ctttccaagc gtcgatcccg ctcggtggcg cgtccgcgcc agatggcgat ggcgctggcg
     5761 aaagagctga ctaaccacag tctgccggag attggcgatg cgtttggtgg tcgtgaccac
     5821 acgacggtgc ttcatgcctg ccgtaagatc gagcagttgc gtgaagagag ccacgatatc
     5881 aaagaagatt tttcaaattt aatcagaaca ttgtcatcgt aaacctatga aatttaccgt
     5941 agaacgtgag catttattaa aaccgctaca acaggtgagc ggtccgttag gtggtcgtcc
     6001 tacgctaccg attctcggta atctgctgtt acaggttgct gacggtacgt tgtcgctgac
     6061 cggtactgat ctcgagatgg aaatggtggc acgtgttgcg ctggttcagc cacacgagcc
     6121 aggagcgacg accgttccgg cgcgcaaatt ctttgatatc tgccgtggtc tgcctgaagg
     6181 cgcggaaatt gccgtgcagc tggaaggtga acggatgctg gtacgctccg ggcgtagccg
     6241 tttttcgctg tctaccctgc cagcggcgga tttcccgaac ctcgatgact ggcagagtga
     6301 agtcgaattt accctgccgc aggcaacgat gaagcgtctg attgaagcga cccagttttc
     6361 gatggcgcat caggacgttc gctattactt aaatggtatg ctgtttgaaa ccgaaggtga
     6421 agaactgcgc accgtggcaa ccgacggcca ccgtctggcg gtctgttcaa tgccaattgg
     6481 tcaatctttg ccaagccatt cggtgatcgt accgcgtaaa ggcgtgattg aactgatgcg
     6541 tatgctcgac ggcggcgaca atccgctgcg cgtgcagatt ggcagcaaca atattcgcgc
     6601 ccacgttggc gactttatct tcacctccaa actggtggat ggtcgcttcc cggattaccg
     6661 ccgcgttctg ccgaagaatc cggacaaaca tctggaagct ggctgcgatc tgctcaagca
     6721 ggcgtttgcc cgtgcggcaa ttctctctaa cgagaaattc cgcggcgtgc gcctgtatgt
     6781 cagcgaaaac cagctgaaaa tcaccgccaa caacccggaa caggaagaag cggaagagat
     6841 cctcgacgtt acctatagcg gtgcggagat ggaaatcggc ttcaacgtca gctatgtgct
     6901 ggatgttctg aacgcgctga aatgcgaaaa cgtccgcatg atgctgaccg attcggtttc
     6961 cagcgtgcag attgaagatg ccgcatcaca gtcggctgcc tatgttgtca tgccaatgag
     7021 actgtaatgt ccctcacccg cttgttgatc cgcgatttcc gcaacattga aaccgcggat
     7081 ctcgctttat ctcccggctt taactttctg gtaggtgcca acggcagtgg caaaaccagc
     7141 gtgctggaag ccatctatac gctcggccat ggtcgggcgt ttcgcagttt gcagattggt
     7201 cgcgtcattc gccatgagca ggaggcattt gttctccatg ggcgattaca gggcgaagag
     7261 cgcgagacgg cgattggctt aaccaaggac aaacagggcg acagcaaagt ccgcatcgac
     7321 ggtactgacg ggcataaagt cgcggaactg gcgcacctga tgccaatgca gctgataacg
     7381 ccagaagggt ttactttact caacggcggc cccaaataca gaagagcatt cctcgactgg
     7441 ggatgctttc acaacgaacc cggatttttc accgcctgga gcaatctcaa gcgattgctc
     7501 aagcagcgca atgcggcgct gcgccaggtg acacgttacg aacagctacg cccgtgggat
     7561 aaagaactga tcccgctggc ggagcaaatc agcacctggc gcgcggagta tagcgccggt
     7621 atcgcggccg atatggccga tacctgtaag caatttctcc ctgagttttc tctgactttc
     7681 tctttccagc gcggctggga gaaagagaca gaatatgctg aggtgctgga acgtaatttt
     7741 gaacgcgatc gccagctaac ctacaccgcg catggcccgc ataaagcgga cttacgcatt
     7801 cgcgccgacg gtgcgccggt ggaagatacc ttatcgcgtg ggcagcttaa gctgttgatg
     7861 tgcgccttac gtctggcgca aggagagttc ctcacccgtg aaagcgggcg gcggtgtctc
     7921 tacctgatag atgattttgc ctctgagctt gatgatgagc gtcgtgggtt gcttgccagc
     7981 cgcttaaaag cgacgcaatc acaggtcttt gtcagcgcga tcagtgctga acacgttata
     8041 gacatgtcgg acgaaaattc gaagatgttt accgtggaaa agggtaaaat aacggattaa
     8101 cccaagtata aatgagcgag aaacgttgat gtcgaattct tatgactcct ccagtatcaa
     8161 agtcctgaaa gggctggatg cggtgcgtaa gcgcccgggt atgtatatcg gcgacacgga
     8221 tgacggcacc ggtctgcacc acatggtatt cgaggtggta gataacgcta tcgacgaagc
     8281 gctcgcgggt cactgtaaag aaattatcgt caccattcac gccgacaact ctgtctctgt
     8341 acaggatgac gggcgcggca ttccgaccgg tattcacccg gaagagggcg tatcggcggc
     8401 ggaagtgatc atgaccgttc tgcacgcagg cggtaaattc gacgataact cctataaagt
     8461 gtccggcggt ctgcacggcg ttggtgtttc ggtagtaaac gccctgtcgc aaaaactgga
     8521 gctggttatc cagcgcgagg gtaaaattca ccgtcagatc tacgaacacg gtgtaccgca
     8581 ggccccgctg gcggttaccg gcgagactga aaaaaccggc accatggtgc gtttctggcc
     8641 tagcctcgaa actttcacca atgtgaccga gttcgaatat gaaattctgg cgaaacgtct
     8701 gcgtgagttg tcgttcctca actccggcgt ttccattcgt ctgcgcgaca agcgcgacgg
     8761 caaagaagac cacttccact atgaaggcgg catcaaggcg ttcgttgaat atctgaacaa
     8821 gaacaaaacg ccgatccacc cgaatatctt ctacttctcc actgaaaaag acggtattgg
     8881 cgtcgaagtg gcgttgcagt ggaacgatgg cttccaggaa aacatctact gctttaccaa
     8941 caacattccg cagcgtgacg gcggtactca cctggcaggc ttccgtgcgg cgatgacccg
     9001 taccctgaac gcctacatgg acaaagaagg ctacagcaaa aaagccaaag ttagcgccac
     9061 cggtgacgat gcgcgtgaag gcctgattgc ggtcgtttcc gtgaaagtgc cggacccgaa
     9121 attctcctcc cagaccaaag acaaactggt ttcttctgag gtgaaatcag cggttgaaca
     9181 gcagatgaac gaactgctgg cagaatacct gctggaaaac ccaaccgacg cgaaaatcgt
     9241 ggttggcaaa attatcgatg ctgcccgtgc ccgtgaagcg gcgcgtcgcg cgcgtgaaat
     9301 gacccgccgt aaaggtgcgc tcgacttagc gggcctgccg ggcaaactgg cagactgcca
     9361 ggaacgcgat ccggcgcttt ccgaactgta cttggtggaa ggggactccg cgggcggctc
     9421 tgcgaagcag gggcgtaacc gcaagaacca ggcgattctg ccgctgaagg gtaaaatcct
     9481 caacgtcgag aaagcgcgct tcgataagat gctctcttct caggaagtgg cgacgcttat
     9541 caccgcgctt ggctgtggta tcggtcgtga cgagtacaac ccggacaaac tgcgttatca
     9601 cagcatcatc atcatgaccg atgcggacgt cgacggctcg cacattcgta cgctgctgtt
     9661 gaccttcttc tatcgtcaga tgccggaaat cgttgaacgc ggtcacgtct acatcgctca
     9721 gccgccgctg tacaaagtga agaaaggcaa gcaggaacag tacattaaag acgacgaagc
     9781 gatggatcag taccagatct ctatcgcgct ggatggcgca acgctgcaca ccaacgccag
     9841 cgcaccggca ttggctggcg aagcgttaga gaaattggtg tctgagtaca acgcgacgca
     9901 gaaaatgatc aaccgcatgg agcgtcgtta tccgaaagca atgctgaaag agcttatcta
     9961 tcagccgacg ctgacggaag ccgacctctc tgatgagcag accgttaccc gctgggtgaa
    10021 cgcgctggtc agcgaactga acgacaaaga acagcacggc agccagtgga agtttgatgt
    10081 ccacaccaat gccgaacaaa acctgttcga gccgattgtt cgcgtgcgta cccacggtgt
    10141 ggatactgac tatccgctgg atcacgagtt tatcaccggt ggcgaatatc gtcgtatctg
    10201 cacgctgggt gagaaactgc gtggcttgct ggaagaagat gcgtttatcg aacgtggcga
    10261 gcgtcgtcag ccggtagcca gcttcgagca ggcgctggac tggctggtga aagagtcccg
    10321 tcgcggcctc tccatccagc gttataaagg tctgggcgag atgaacccgg aacagctgtg
    10381 ggaaaccacc atggacccgg aaagccgtcg tatgctgcgc gttaccgtta aggatgcgat
    10441 tgccgctgac cagttgttca ccacgctgat gggcgacgcc gttgaaccgc gccgtgcgtt
    10501 tattgaagag aacgccctga aagcggcgaa tatcgatatt taatggcgct aaccatgcga
    10561 gcgtgcctga tgcgctacgc ttatcaggcc tacgaaaatc ttgcaatgta ttgaattttc
    10621 atgattttgt aggccggata aggcgttcac gccgcatccg gcaagaagct ataagaaaag
    10681 ggcggagatc atctccgccc tttttatttc tgcaatccgc aattatcatc ccgcaaatct
    10741 atactttttg ggtcaacacg taacgcttta caggtgatta tcatggggct ttttgatgaa
    10801 gttgtcggtg cctttctgaa aggcgatgcg gggaaatatc aggctatttt aagttgggtt
    10861 gaggagcagg gcggcattca ggtgctgctg gaaaaactgc aaagtggcgg cttaggggcc
    10921 attctctcaa cctggctgag taatcaacag ggcaatcaat cggttagtgg cgagcaactg
    10981 gaatcggcgc tcggcacaaa tgcggtgtcc gatcttggac aaaaacttgg cgtggatacc
    11041 agtacagctt ccagtttact ggcagaacaa ttgccgaaga ttattgatgc gctctcaccg
    11101 caaggtgaag tgtctccaca agccaataac gatctgcttt ccgcaggcat ggaactgctg
    11161 aaagggaaac tcttccgcta agcaaatggg gagcatgcaa cccgcgttgc atcccccttt
    11221 tccctcgggt gttgtactga tttttgagcg gaatcgcgtt agcatgggtc aggaactaat
    11281 ctacctgggg aactcatggc tatcaaactc attgctatcg atatggatgg cacccttctg
    11341 ctacccgatc acaccatttc acccgccgtt aaaaatgcga ttgccgcagc tcgcgcccgt
    11401 ggcgtgaatg tcgtgctaac gacgggtcgc ccttatgcag gtgttcacaa ttacctgaaa
    11461 gagctgcata tggaactgcc gggcgactac tgcattactt ataacggcgc gctggtacag
    11521 aaggccgctg atggtagcac cgtggcgcaa accgctctca gctatgacga ctaccgtttc
    11581 ctggaaaaac tctctcgcga agtcggttct catttccacg ccctggaccg caccacgctg
    11641 tacaccgcca accgtgatat cagctactac acggtgcatg aatccttcgt tgccacaatt
    11701 ccgctggtgt tctgcgaagc ggagaaaatg gaccccaata cccagttcct gaaagtgatg
    11761 atgattgatg aacccgccat cctcgaccag gctatcgcgc gtattccgca ggaagtgaaa
    11821 gagaaatata ccgtgctgaa aagtgcgccg tacttcctcg aaatcctcga taaacgcgtt
    11881 aacaaaggta cgggggtgaa atcactggcc gacgtgttag gtattaaacc ggaagaaatc
    11941 atggcgattg gcgatcagga aaacgacatc gcaatgattg aatatgcagg cgtcggtgtg
    12001 gcgatggata acgctattcc ttcggtgaaa gaagtggcga actttgtcac caaatccaac
    12061 cttgaagatg gcgtggcgtt tgctattgag aagtatgtgc tgaattaatc agtggacggg
    12121 caaacagcat aaacgcgttt gcccgcttac tgattattgg tttactgcat atacaaccag
    12181 aaattgcagc ggtgcagtaa tgatgtccag cgtaacagtc actggcagta atgctgcgga
    12241 aacaccatcc ggaatgcctc tgggtgaata gtattcataa aatcgcactt taaacggatg
    12301 atagaacgcc atcacctttg agtaatccat gtttttattt ttctcatgga ttgtgccttt
    12361 taggttttcc agtgacctgg tacagtgttg aacaccacag gcaaaaccat aatgcgttgc
    12421 cagtgccttt tcttcttcgt tattccagtg gtagtaaagc gatattgtgc cggtaaattt
    12481 ctttttcccc gcatttaaaa caaatcttgc gtcatcggca acctgaatat tgtgccggtt
    12541 aagtgctgga tcattgagca tcttaacgat gtcatcggca ccatcagtga taaggtaatc
    12601 cagcgattcc cctacaaaaa catagccttt tgttccatta ctatcttttg cctgcgatag
    12661 cccagtgatg gtgtctgact gcgattgata ctttcctgaa ctaccctttg cggtatggga
    12721 aaccagagca tacgagatac agcctgaaag catcagcgcc agtgggaata aaccggcagc
    12781 cttaaaaaat cccttaaaat tcaacttcat gattaatcct ttaaacgagt agctaagccg
    12841 ttgaaagcat actcttcttt ccctcctgtt tagcgcctga tatccctttt cagatttctg
    12901 cccgacgcat gtcatttttt tatgcattgt tctttttgtg atctaaattg tagtacaaca
    12961 atataagttt gtactacatt acgcacacgg caaacgcgaa cgtcatcacg ctggtactac
    13021 aaagttgccg cgttatgcat cgatcggggt aaagtagaga agaacataca gagcacaagg
    13081 actctccatg actctcaata aaaccgatcg cattgtcatt acgctgggta aacagattgt
    13141 tcacggcaaa tacgtacctg gctcgccact tccggctgag gcggagctct gtgaagagtt
    13201 tgcaacctcg cgcaacatca tccgtgaggt gttccgttcg ttgatggcga aacggctgat
    13261 tgaaatgaaa cgttatcgcg gcgcgtttgt ggcaccgcgt aaccagtgga attacctcga
    13321 cactgacgta ctgcaatggg tgctggaaaa cgactacgac ccacggctta tcagtgccat
    13381 gagcgaagtg cgaaatctgg tggaaccggc gattgcccgt tgggcagcag agcgcgcgac
    13441 ttccagcgat ctggcgcaga ttgaatcggc gctgaacgag atgattgcca acaatcagga
    13501 ccgcgaagcg tttaacgaag cggatattcg ctaccacgag gcggtgctgc agtcggtaca
    13561 taacccggtg ttacagcaac ttagcattgc gatcagttcg ttgcagcggg cggtttttga
    13621 acgaacctgg atgggcgatg aggccaacat gccgcaaacg ctccaggaac ataaggcgct
    13681 gttcgatgca atacggcatc aggacggcga tgcggcagag caggcggcgc ttaccatgat
    13741 cgccagctcg acacgaaggt taaaggaaat cacatgacag ctcgctacat cgcaattgac
    13801 tggggatcga ccaatcttcg cgcctggctt tatcagggcg accactgcct ggagagcagg
    13861 caatcagaag caggcgtcac gcgcctgaac ggaaaatctc cggctgcggt gttagcagaa
    13921 gtcacgaccg actggcgtga agagaatacg ccagtggtaa tggcaggaat ggtcggcagc
    13981 aatgtcggct ggaaagttgc tccgtattta tctgttcctg cccgtttttc gtctattggc
    14041 gaacaattaa cgtctgttgg cgacaatatc tggattattc ccggattatg cgtctctcat
    14101 gacgataacc acaatgtgat gcgcggcgaa gaaacacaat tgatcggcgc gcgaactctg
    14161 gctccttcct ctctttatgt tatgcccggt acgcattgca aatgggtgca ggccgatagc
    14221 cagcaaatca acgattttcg caccgtgatg accggtgaat tacatcattt actgttaaat
    14281 cactcattga ttggcgcagg tttgccgccg caggaaaact ctgccgatgc cttcgcggct
    14341 ggccttgagc gcggccttaa tgcgcccgcc atattgccgc agctttttga agttcgcgcc
    14401 tcgcatgtgc tgggaacact tccccgcgaa caggtcagcg aatttctctc tggtttgttg
    14461 attggcgcag aggttgccag tatgcgcgac tatgtgaccc atcaacacgc catcaccctt
    14521 gtcgccggaa catcgctgac cgcgcgctac cagcaagcct ttcaggcgat gggttgcgac
    14581 gtgacggcgg tggcgggcga cacggcattt caggctggta taaggagcat cgctcatgca
    14641 gtggcaaact aaacttccgc tgatcgccat tttgcgcggc attaagcccg acgaggcgct
    14701 ggcgcatgtt ggcgcggtga ttgacgccgg gttcgacgcg gttgaaatcc cgctgaattc
    14761 cccacaatgg gagcaaagca ttcccgccat cgttgatgcg tatggcgaca aggcgttgat
    14821 tggcgcaggt acggtactga aacctgaaca ggtcgatgcg ctcgccagga tgggttgtca
    14881 gctcatcgtt acgcccaata tccatagtga agtgatccgc cgtgcggtgg gctacggcat
    14941 gaccgtctgc cccggctgcg cgacggcgac cgaagccttt accgcgctcg aagcgggcgc
    15001 gcaggcgctg aaaatatttc cgtcatcggc ttttggtccg caatacatca aagcgttaaa
    15061 agcggtattg ccatcggaca tcgcagtctt tgccgttggc ggcgtgacgc cagaaaacct
    15121 ggcgcagtgg atagacgcag gttgtgctgg ggcgggctta ggcagcgatc tctatcgcgc
    15181 cgggcagtcc gtagaacgca ccgcgcagca ggcagcagca tttgttaagg cgtatcgaga
    15241 ggcagtgcaa tgaaaatcac caaaattacc acgtatcgtt tacctccccg ctggatgttc
    15301 ctgaaaattg aaaccgatga aggcgtggtc ggttggggcg agcccgtgat tgaaggccgc
    15361 gcccgtacgg tggaagcggc agttcacgag ctgggtgact atttgattgg tcaggatcct
    15421 tcgcgcatca atgacttatg gcaagtgatg tatcgcgccg gattttatcg tggcggtcca
    15481 atcctgatga gcgccattgc cgggatcgac caggcgttat gggatatcaa aggcaaagtg
    15541 ctgaatgcgc cggtctggca actgatgggc ggcctggttc gcgacaaaat taaagcctac
    15601 agttgggtcg gcggcgatcg tccggcggat gttatcgacg gcattaaaac cctgcgcgaa
    15661 atcggcttcg ataccttcaa actgaacggt tgtgaagaac tggggctaat tgataactcc
    15721 cgcgcggtag atgcggcagt caacaccgtg gcacaaattc gtgaagcttt tggcaatcag
    15781 attgagtttg gtcttgattt ccacggtcgc gtcagcgcgc caatggcgaa agtgctgatt
    15841 aaagaactgg agccgtatcg cccgctgttt attgaagagc cggtgctggc ggaacaagcc
    15901 gaatactacc cgaaattggc ggcacaaacg catattccac tggcggcagg tgagcgcatg
    15961 ttctcacgtt tcgattttaa acgcgtgctg gaggcaggcg gtatttcgat tctgcaaccg
    16021 gatctctccc atgcaggcgg tattaccgaa tgctacaaaa ttgctggaat ggcagaagcc
    16081 tatgatgtga cccttgcgcc gcactgtccg ctcggaccga ttgcactggc agcttgcctg
    16141 catatcgact ttgtttccta taacgcggta cttcaggaac aaagtatggg cattcattac
    16201 aacaaaggcg cggagttact cgactttgtg aaaaacaaag aagacttcag tatggttggc
    16261 ggcttcttta aaccgttaac gaaaccgggc ttaggtgtgg aaatcgacga agctaaagtt
    16321 attgagttca gtaaaaatgc cccggactgg cgtaatccgc tctggcgtca tgaagataac
    16381 agcgtagcag agtggtaatt cctgccacgt aagcccctca tcgggcacta aaacagcaat
    16441 acaaaaatat aaccctctgt aaattacagg gcatggtgag cggcttcgct atgcccaaaa
    16501 tctggagaaa gctcgctatg gatattcccg ttaatgcagc aaagccgggg cgtcggcgtt
    16561 atctgacgct ggtgatgatc tttattacgg tggtcatttg ttatgttgac cgcgctaacc
    16621 tggccgtggc ttccgcccat attcaggaag agttcggcat tacgaaagcg gaaatgggct
    16681 atgtattttc ggccttcgcc tggctttata cgctatgcca gatccccggc ggttggtttt
    16741 tagatcgcgt aggttctcgc gtgacttatt ttattgcgat atttggttgg tcagtggcga
    16801 ctttattcca gggctttgcc acgggattaa tgtcattaat tggtctgcgt gcgataaccg
    16861 gtattttcga agcgcccgct ttcccgacca ataaccggat ggtgaccagc tggttcccgg
    16921 aacatgaacg cgcttccgcc gttggttttt atacgtctgg tcagtttgtc ggtctggcgt
    16981 ttttgactcc gctgctgatc tggattcagg agatgttgag ctggcactgg gtgttcattg
    17041 tcactggtgg tatcggcatt atctggtcgc tgatttggtt taaggtttat cagccgccgc
    17101 gcctgaccaa aggtatcagc aaagctgaac tggattacat tcgtgatggc ggcggtctgg
    17161 tggatggtga tgcgccggtg aagaaagagg cgcgtcagcc gttaacagcc aaagactgga
    17221 aactggtgtt ccatcgtaaa ctgatcggcg tttatcttgg gcaatttgcg gtggcttcta
    17281 cactgtggtt tttcttaacc tggttcccga actatttaac ccaggaaaaa ggaatcacgg
    17341 cgctgaaagc aggctttatg accacggtgc cattcctcgc ggcgtttgtc ggcgtcctgc
    17401 tctctggctg ggtcgcggat ctgctggtac gtaagggctt ttcactgggc tttgcgcgta
    17461 aaacgccgat tatctgcggc ttgctgatct ccacctgcat tatgggcgct aactacacca
    17521 acgatccgat gatgattatg tgcctgatgg cgctggcatt cttcggcaac ggttttgctt
    17581 cgattacctg gtcgctggtc tcttctctgg caccgatgcg cctgattggt ttaaccggcg
    17641 gcgtgtttaa cttcgccggt ggtctgggcg gcatcaccgt tccgctggtg gtggggtacc
    17701 tggcgcaggg ttacggtttc gcacctgcac tggtttatat ctccgccgtc gcgttgattg
    17761 gcgcgctctc ttacatcctg ctggtgggcg atgtgaagcg cgttggctaa tctttcaact
    17821 gtggaatatg cgccatacca ctgcgcataa tccgctttct taaagccggt gcggtcaggc
    17881 atcggctttt tactatcttg ccgaagagtt ttaaacgcag tttgcgggtg gcgcgccagt
    17941 aagcggtatt gagcttctct ggctgcttca gtaacaccga acgcagaatg tctgcgctat
    18001 ccagcgcata gctaatcccc tccagcgaac tggcgctgat aaatcctgcc gcttcgccaa
    18061 tcagaaaggc gttgtcctta ccgcagacaa aatcctgcca gcgtgaggga aacagcaccg
    18121 tgcatttttc gcttttcacc gccttaccaa actggaactg aaaggcgctc attttctctt
    18181 tcagcgtcgt gaaacgcgtc tgaccgtctt tcattggata agcaccgcca aagataaaat
    18241 aaccgtcttt gctgatactc catgaataac agttagttat cgaattatca aagatgcagg
    18301 agtagaacgg caccggatgt ttctctgcga accactgctg gatagcgaca tatttacgga
    18361 tttgatgatc cgggtagaga tgtcgccgca ccatcgagtt tgcgccatcg gcaccgacca
    18421 gatagcgggc agtaatatgt tgctcccagc cgtctgcacg aaaaatgaca tgccatttat
    18481 catcctcacg ccagattttt cgacacaggc tgtcgtgata aatctcaacg ctggcgggga
    18541 tcagcgattt catccacaag tcgaaagcat ggcgattaat attgatatag cttcgctggt
    18601 agttacgcgt gagcgatgcg gcgacgtcga cagttttgac gctgaaaatc tgcgggttgg
    18661 cgatcacatc gacaggaagc gtcagtccat cgcgaataaa agatcgctgc gcgtccggtg
    18721 ccagcagacc gccgcaaggt ttgctgaaac cttcagtacc acactggtgc tttttatcca
    18781 gcgcgatcac ctgcattttg cctgctaatt ttcgcgccaa cgccgatcct gccgggccga
    18841 ggccaataat cgccacgtcg aaatgttcca tcttttcact cacattcatc acgatggcaa
    18901 aagactattg tctgaaagtg aagtccttgt gaagtatgat ggcgggaccc gttttgtatc
    18961 aggaccgcgc catgaaacag ataacctttg ctccccgtaa tcacctgctc accaatacca
    19021 atacctggac gcccgacagc cagtggctgg tatttgacgt gcgtccttct ggggcgtcgt
    19081 ttaccggcga gaccattgag cgtgtgaata tccataccgg cgaggtcgag gttatctatc
    19141 gcgcgtcaca gggcgcacac gtcggcgtgg tgaccgttca tccgaagtca gagaaatatg
    19201 ttttcatcca tggcccggaa aatcctgatg aaacatggca ttacgatttc catcatcgac
    19261 gcggagtcat tgctgaaggc ggcaaggtga gcaatctcga tgcaatggat attaccgcgc
    19321 cgtatatgcc aggagcgctg cgcggcggca gccatgtgca tgtctttagc ccgaacggtg
    19381 aaagggtgag ctttacctat aacgaccatg taatgcatga actcgatccg gcgctggatt
    19441 tgcgaaacgt cggtgttgct gcgccgtttg gcccggtcaa cgtacaaaag cagcatccgc
    19501 gtgaatacag cggtagccac tggtgcgtgc tggtgagtaa aaccacgccc acgccgcagc
    19561 ctggcagcga tgaaatcaat cgtgcttatg aagaaggatg ggtaggaaat cacgcgctgg
    19621 cgtttattgg cgacacactt tcgccaaagg gcgagaaagt gccggagctg tttatcgttg
    19681 agttaccgca agatgaagct ggctggaaag cggcaggtga tgcgccgtta agtggaacgg
    19741 aaaccaccct gcccgcgcca ccgcgcggcg tcgtgcagcg acgtttaacc tttacccacc
    19801 atcgggctta tccggggtta gtcaacgtcc cgcgccactg ggtgcgctgt aatccgcagg
    19861 gtacgcaaat cgcgttttta atgcgtgatg ataacggcat tgtgcaactg tggcttatct
    19921 cgccacaggg cggcgagccg cgccagttaa cccataacaa aacggatatt cagtctgcat
    19981 ttaactggca tccgtcagga gaatggttgg gctttgtgct ggataatcga attgcttgtg
    20041 cccatgcgca aagcggcgag gttgagtatt taaccgaaaa ccacgccaat ccaccttctg
    20101 cggacgccga ggtcttctcg ccggatggtc aatggctggc gtggatggaa ggtggccagc
    20161 tgtggatcac cgaaactgat cgctaaatca gttatcaggc attctggccg gtggaataac
    20221 cgcattggtg gcttgcgcgt tggcttcgct tttttcaacg cgtgagcgca ccgagctgtc
    20281 tttgcggaac acatcccacg gcagtaaaag cgtgtccatc acagcggtaa acggcatatc
    20341 gagaatcgcc agcgacttag tgccccagtt ggtttcatcg tcgccaatca tggttgcgct
    20401 ggcgcgggtg ccgggatacg ttccttcttt ccctccggta tgggacatca cgctggagca
    20461 tcctccgagt aatgtcattc cgctgcatat catgaacgct aacaacacat ttcttatcat
    20521 aataaattca tctgttgatc gtgggtgttg gcctgatgag ttatagcgat cccttgctga
    20581 aaataacatc atcattacgt cgcactgtgg cggctatcgc actttaacgt ttcgtgctgc
    20641 cccctcagtc tatgcaatag accataaact gcaaaaaaaa gtccgctgat aaggcttgaa
    20701 aagttcattt ccagacccat ttttacatcg tagccgatga ggacgcgcct gatgggtgtt
    20761 ctggctacct gacctgtcca ttgtggaagg tcttacattc tcgctgattt caggagctat
    20821 tgattatgcg taactttgat ttatccccgc tttaccgttc tgctattgga tttgaccgtt
    20881 tgtttaacca cttagaaaac aaccagagcc agagtaatgg cggctaccct ccgtataacg
    20941 ttgaactggt agacgaaaac cattaccgca ttgctatcgc tgtggctggt tttgctgaga
    21001 gcgaactgga aattaccgcc caggataatc tgctagtggt gaaaggtgct cacgccgacg
    21061 aacaaaaaga gcgcacctat ctgtaccagg gcatcgctga acgcaacttt gaacgcaaat
    21121 tccagttagc tgagaacatt catgttcgtg gcgctaacct ggtaaatggt ttgctgtata
    21181 tcgatctcga acgcgtgatt ccggaagcga aaaaaccgcg ccgtatcgaa atcaactaat
    21241 tccctaaggc cgcctggcgc ggcctgacat ctccatgctc gccgttaggg agcatatgcg
    21301 aatcttcgga tttgcaggta cttactcgct tcttagaagg agaaatgact atgcgtaact
    21361 tcgatttatc cccactgatg cgtcaatgga tcggttttga caaactggcc aacgcactgc
    21421 aaaacgccgg tgaaagccag agcttcccgc cgtacaacat tgagaaaagc gacgataacc
    21481 actaccgcat tacccttgcg ctggcaggtt tccgtcagga agatttagag attcaactgg
    21541 aaggtacgcg cctgagcgta aaaggcacgc cggagcagcc aaaagaagag aaaaaatggc
    21601 tgcatcaagg gcttatgaat cagccattta gcctgagctt tacgctggct gaaaatatgg
    21661 aagtctctgg cgcaaccttc gtaaacggtt tactgcatat tgatttaatt cgtaatgagc
    21721 ctgaacccat cgcagcgcag cgtatcgcta tcagcgaacg tcccgcgtta aatagctaac
    21781 tagctattct ctttgccccg ccattctggt ggggcttttt ttgtcttact tactcaacaa
    21841 tgcgttaaat ttcgactgtt taagatattt cggcacgttc tttcccgtga taatgtgcgc
    21901 tataaggcaa atctgcttca ccgattcagc cataatccgt agcaattaaa aatgttatct
    21961 caagagaaat ggacgatgag tgatatagca ttaacggtca gtattctggc tttggtggca
    22021 gtcgtcggtt tgtttatcgg caatgtcaaa tttcgcggca taggattagg tattggcggc
    22081 gtgctgtttg gtgggatcat cgtcggccat tttgtttctc aggcggggat gacattaagt
    22141 agcgatatgc tgcatgttat tcaggaattt ggcctgatcc tgttcgttta tactatcggg
    22201 attcaggtag ggccgggctt ctttgcctca ttgcgcgtct ccggattacg cctcaacctg
    22261 tttgctgttc tgatcgtcat catcggtggt ctggttaccg ccatcctgca taaactgttt
    22321 gatattccac tgccggtagt gctggggatt ttctccggtg cggttaccaa tacgccagcg
    22381 ctgggggcag ggcagcagat tttgcgcgac ttgggtacac caatggaaat ggtcgatcag
    22441 atggggatga gttacgcgat ggcgtatcca ttcggcattt gcgggatttt gttcaccatg
    22501 tggatgttgc gggttatttt ccgcgtcaat gtcgagacag aagctcagca gcacgagtct
    22561 tcacgcacca atggcggcgc gctgatcaag actatcaata ttcgcgttga gaaccctaac
    22621 ctgcatgatt tagccattaa agatgtaccg attctcaacg gcgacaaaat tatctgctcg
    22681 cgtctgaaac gcgaagaaac cctaaaagtt ccttcgccag ataccattat ccaactgggc
    22741 gatttgctgc atctggtggg gcagccagcg gatttacata atgcgcaact ggtgattggt
    22801 caggaggtcg atacttcgct gtccacgaaa ggcactgatt tgcgcgtcga gcgtgtggtg
    22861 gtcaccaatg aaaacgtgct cggaaaacgt attcgcgacc tgcactttaa agaacgctat
    22921 gacgttgtta tctcgcgcct gaaccgtgcc ggggtcgaac tggtcgccag tggcgatatc
    22981 agcctgcagt tcggcgatat cctcaatctg gtggggcgtc cgtccgcaat tgatgccgtt
    23041 gccaatgtgc tggggaatgc gcagcaaaaa ctgcaacagg ttcagatgct gccagtgttt
    23101 attggcatcg ggcttggcgt attgttaggt tctattcccg tctttgtgcc tgggttcccg
    23161 gccgcgttga aactggggct ggcgggcggt ccgctgatta tggcgttgat cctcgggcgt
    23221 atcggcagta tcggcaagct gtactggttt atgccgccaa gtgccaacct cgcgctgcgg
    23281 gagctgggga tcgtgctgtt cctctcggtc gttggtctga aatctggtgg agattttgtg
    23341 aataccctgg tcaatggcga agggctaagc tggattggtt atggtgccct gatcaccgcc
    23401 gttccgctga ttactgttgg cattctggcg cggatgttag ccaaaatgaa ttacctgacc
    23461 atgtgcggga tgctggcagg ttccatgacc gatcctccgg cgctggcgtt tgctaataat
    23521 cttcatccaa ccagcggtgc ggcggcgctc tcttacgcca ctgtctatcc gttagtgatg
    23581 ttcctgcgca ttatcacccc ccaattactg gcggtgctct tctggagtat cggttaactc
    23641 ttttccgggt ggagtcgccg taaatggtac tccacctggt attcgctggc atttctgaac
    23701 atcactgaat aattcaaaaa ctccccgctc tcgctatatg acaacgaggt gatccgcagc
    23761 agtggcgtgt gttccgctac cttcatttgc cgcgccagca gtctatcggc gagcactggc
    23821 gtcaggcttt cataattacc gccaatcaaa atcccgcact cttgttcaat atattcaaac
    23881 ttcgaccctt ccagatgttg cagcgaaaga ttacggaaca gttttaccgg catatagctg
    23941 tcttccagca tcagcggttt cccttccaca aaacgaacgc gacgggagaa gtagatctgc
    24001 tcgttgattt gaatccgtaa ctggctggca atggccggag gcgcaggcat gatttcaaaa
    24061 atcagcacct ggctggtgac cgtttttccc tgccgtttta acacctccac cagcccggtc
    24121 aggctggcgg tttgatgcag tacatctttg cgcaccaggt aagtgccgct gccgtggcgg
    24181 cggaccacca atccccacgc taccagcagg tcaatggctt tacggatggt catccgcgat
    24241 accgcaaatt cttccgccaa ctttttttca ccgggaagaa ggctatttag cgtgaaatct
    24301 gcggaattaa gtcgaattct taaccgctcc gcaatgcttt tgtagatcat ctctagatct
    24361 cttttttgcc tgcaagcacg ggtaatgcgc ccacaagttc tgcttaatcg attgaaaaaa
    24421 catctttttt ttaaagatgt gttcgatgct gtgtacctgt gctcacagat gtctactttt
    24481 tcgcgaaaac gtagatctct accgcccaac gaaaagcatg aaagcgatca cgaatcccat
    24541 tgggtcgtca tgttctgccg atcgcatctt cctatcctcg ctccaggcct gccgcataac
    24601 caatcaggct tcctacttac agaattgaga aaagaggatg tggaaatgct cagtcaaatt
    24661 caacgctttg gcggcgcgat gttcacgcca gtgctgctgt ttcccttcgc cgggattgtg
    24721 gtgggtcttg ccatcttgct gcaaaacccg atgtttgtcg gggaatcact gaccgatccg
    24781 aacagtttat tcgcgcaaat cgtacacatt attgaagagg gcggttggac ggtgttccgt
    24841 aatatgccgc tgatttttgc tgtcggttta cccattggcc ttgctaagca agcgcagggg
    24901 cgtgcttgtc tggcggtgat ggtgagtttc ctgacttgga actatttcat caacgcgatg
    24961 ggaatgacct ggggaagcta cttcggcgtc gatttcactc aggacgcggt ggcaggtagc
    25021 ggtctgacaa tgatggccgg gattaaaacc ctcgatacca gcattatcgg cgcaattatc
    25081 atttccggca ttgtgacggc gctgcataac cgtctgttcg ataaaaaact gccggttttt
    25141 ctcggcattt tccaggggac gtcttatgtg gtgattatcg ccttcctggt gatgatcccc
    25201 tgtgcctggc tgacgttgct cggctggcca aaagtacaaa tggggattga atctctgcaa
    25261 gcgttcctgc gttcggcggg tgcgcttggg gtgtgggttt acaccttcct cgaacgtatt
    25321 ctgatcccaa ccggtttaca ccacttcatc tacggaccgt ttatctttgg tccggcagct
    25381 gttgaaggcg gcattcagat gtactgggcg cagcatctgc aagagttcag tctgagcgcc
    25441 gagccgctga aatcgttgtt cccggaaggc ggttttgccc tgcacggtaa ctcaaaaatc
    25501 tttggtgccg tgggcatttc tttagcgatg tacttcactg ccgcaccgga aaatcgggta
    25561 aaagtggcgg gcttgctgat tcccgcaacc ttaaccgcca tgctggtggg aattaccgaa
    25621 ccgctggaat ttaccttcct gttcatttca ccgttgctgt ttgcggtaca cgccgtgctg
    25681 gcggcctcaa tgtcgaccgt gatgtatctc tttggtgtgg tgggcaacat gggcggaggt
    25741 ctgattgacc aggttttacc gcaaaactgg atcccgatgt tcagcaacca cgcggatatg
    25801 atgctgaccc aaatcgccat tgggttgtgc tttaccctgc tgtacttcgt ggttttccgc
    25861 accctgattc tgcagttcaa catgtgcacg ccgggacgtg aagatgcgga agtgaaactc
    25921 tactcaaaag ccgaatacaa agcctcgcga ggccaaacca ccgcggcaga gccaaaaaaa
    25981 gagctggatc aggctgccgg tattctgcaa gccctgggcg gggtcggcaa tatctccagc
    26041 attaacaatt gcgcgacgcg tctacgcatt gcactgcatg acatgtcaca aacgctggat
    26101 gacgaagtct ttaaaaagct gggagcgcac ggcgtcttcc gtagtggcga tgccattcag
    26161 gtgatcattg gtctgcatgt atcccaattg cgtgaacagc tcgatagctt aattaattct
    26221 catcaatcag cagaaaatgt tgccattacg gaggcagtat aatgaccaaa ttctcagtgg
    26281 ttgtcgcagg cggtggaagc acctttacgc caggcatcgt gttgatgctc ctggcgaatc
    26341 aggaccgttt cccgcttcgt gcactgaaat tttatgataa cgatggcgcg cggcaggaag
    26401 tgattgccga agcctgtaaa gtcatcctta aagaaaaagc gccggacatt gcgtttagtt
    26461 acaccaccga tcctgaagta gcgttcagcg acgttgattt tgtcatggcg cacatccgcg
    26521 tcggcaagta cccgatgcgc gaactggatg aaaaaatccc actgcgccac ggtgttgttg
    26581 gtcaggaaac ttgcggaccc ggcggaatag cgtacggcat gcgttccatt ggcggcgtcc
    26641 tggaactggt ggattatatg gaaaaatatt caccaaacgc ctggatgctc aactactcca
    26701 acccggcagc aattgtcgca gaagccacgc gtcgtctgcg cccgaatgcg aaaatcctca
    26761 acatctgtga catgccaatc ggtattgaaa gccggatggc gcaaattgtt gggctgcaag
    26821 atcgcaaaca gatgcgcgtg cgctactacg gcctgaacca ctggtggtcg gcgatatccc
    26881 gcagtttcag aaagggttaa tgagtcagca agtggcggtg gaaaaactgg tcgtggacgc
    26941 ctgggaacag cgttcatatc agcacctgtg gcaggcgatt acgttgtcga aaacggtacc
    27001 gagcgcctcg gtcgccaaag ctattctgga tgagttgctg gaggccaaca aagcgtactg
    27061 gccagagtta cgttaattgt gtggactggc atccgttggg tgctggtccg tattctttag
    27121 cacggctgac ggcgcataac caaatttatg tttaaaggct ttgctgaaat gaaaggagtc
    27181 gaagaagttg agcgtatcag cgacttcgcc aaccgagttt ccttgttgct ttaatagcga
    27241 tagcgccaga tccagacgcg catcgaggta atattctttc ggcgtcttgc cggtatagcg
    27301 taaaaacaga cgacgcaacc aggcttcgct acaggggatc gtggcagcca tatcagctac
    27361 gctccagcgt tgttgcagac tggcatgtaa agtggcaatt aatttttcaa tttgccgccg
    27421 ttgtggatct tttttaccat ctgcgtacat aagacaaatc cactgataaa taattttcgt
    27481 cagaaaagcg actgccagat tattttttat tgcttctggt gaagttatta actcagcaac
    27541 ttcggtgagt tcctgattat aaatttcgcc attataaata acgctttgct gaccaacggg
    27601 aatatccatc atactggtgg gggtaaattc catccagtac tgttcccaga ctaaaccttc
    27661 acagtgataa gagtgaatat ccattggctt taagaatata atacagttgc catgcagtgt
    27721 tatttgttcg ccattcttta aaagaatttt cccacatcct tgtacggtat acaccgcaac
    27781 ccatgagttg gcgataagcg gtttcttctt gtcccgcggc cagatcaccc ggtaatgctc
    27841 gtcggcgtag gtatgccaga gcgagatcag agaaattccg ctggagatca cattttcgtt
    27901 aatcaataag ggaatattgt acggagtttc gttttttaca tctgaacttt gcaattttcc
    27961 attcatttta atatccctcc ggcgaattat gctcacaacc tgaacttaac agaaaacaat
    28021 ccgttcaggg atacgacgtt tgtattttaa gaatctgact gcctgacccg acgcatttta
    28081 cctctcccta tattcatgcg tccgggacat aaacataaat aagggctatg agatgaattc
    28141 gttacaaatc ttgagttttg tcggttttac gctgctggtg gcggtgatca cctggtggaa
    28201 ggtccgcaaa acagataccg gatcgcaaca aggctatttt cttgccggac gttcactaaa
    28261 agcgccggtt attgccgctt cgttaatgct aaccaacctt tccacggaac aactggtcgg
    28321 cctttccggg caggcctaca aaagcggcat gtcggtgatg ggctgggaag tgacttcagc
    28381 ggtgacgctg atcttcctcg cgctaatctt tttaccgcgc tatctgaagc gcggcattgc
    28441 caccatcccc gattttctgg aggaacgtta tgataaaacg acgcgtatta tcatcgactt
    28501 ctgcttcctc attgccaccg gcgtctgctt tctgccgatt gttctctact ccggcgcgtt
    28561 ggcgctcaac agcctgtttc acgtcgggga atcgctacag atttctcacg gtgcggctat
    28621 ctggctactg gtaattttgc ttggtctggc gggaattttg tatgcggtga tcggcggact
    28681 gcgcgcaatg gcagtggcgg actccatcaa cggtattggg ctggttatcg gcgggttgat
    28741 ggtgccggta tttggcctga tcgcgatggg caagggcagc tttatgcagg gcattgagca
    28801 actcaccacc gttcacgccg agaaattaaa ctcaatcggt ggcccgaccg atcccttgcc
    28861 gattggcgcg gcatttaccg gtttgattct ggtgaacacc ttttactggt gtacaaatca
    28921 gggcatcgtg caacgcacgc tggcgtcaaa aagcctggcg gaagggcaaa agggggcgct
    28981 gttaacggcg gtgctgaaaa tgctcgaccc gctggtactg gtgctgccag ggttgattgc
    29041 gtttcatctg tatcaggatt taccgaaagc cgacatggcc tacccgacgc tggtcaataa
    29101 cgttctgcca gtgccaatgg tgggtttctt cggcgcggtg ttatttggtg cggtgatcag
    29161 taccttcaac ggctttctga atagcgccag tacgttattc agtatgggta tttaccgtcg
    29221 catcattaac cagaatgccg agccgcagca gctggtcacc gtcgggcgca aatttggttt
    29281 ctttatcgct atcgtttcgg tgctggtcgc gccgtggatc gccaacgcgc cgcaggggct
    29341 gtatagctgg atgaaacagc tcaacggcat ttacaacgtg ccgctggtta ccatcatcat
    29401 tatgggcttt ttcttcccgc gcatcccggc gctggcggca aaagtggcga tggggattgg
    29461 cataatcagc tacatcacca tcaactatct ggtgaagttc gacttccatt tcctctatgt
    29521 gctggcctgt acgttctgca tcaacgtggt cgtgatgctg gtgatcggtt ttatcaaacc
    29581 gcgcgccacg ccgttcacct tcaaagatgc gtttgcggtg gacatgaaac cgtggagaaa
    29641 cgtcaagatc gcgtcaattg gcatcctgtt cgcgatgatt ggcgtctatg ccgggctggc
    29701 tgaattcggc ggctacggta cgcgctggtt agcgatgatc agttatttca ttgccgccgt
    29761 agtgattgtc tacctgattt ttgacagctg gcggcatcgt cacgacccag ccgtaacctt
    29821 tactcccgac gcgaaggata gcctatgaaa cgccccaatt ttctgttcat catgaccgat
    29881 acccaggcca ccaatatggt cggttgctat agcggtaaac cgctgaatac gcaaaatatt
    29941 gatagtctgg cggcggaagg tattcgcttt aattccgcct acacctgttc accggtttgt
    30001 acgcctgcac gcgccggact atttaccggt atctacgcta accagtccgg cccgtggacc
    30061 aacaacgtcg cgccaggcaa aaacatctcc actatggggc gctactttaa ggatgccggc
    30121 tatcacacct gttacatcgg caaatggcat ctcgacggtc atgactattt cggcactggc
    30181 gagtgtccgc cggagtggga cgctgattac tggttcgatg gggcgaacta ccttagcgaa
    30241 ctgacggaaa aagagatcag cctgtggcgc aatggcctaa acagcgttga ggatttacag
    30301 gcgaaccata tcgacgaaac cttcacctgg gcgcaccgca tcagcaatcg ggcagtagat
    30361 tttctgcaac agcccgcgcg cgccgaggaa cccttcctga tggtggtttc gtatgatgag
    30421 ccgcatcacc cgttcacctg tccggtggag tatttagaga aatacgctga tttttactac
    30481 gatctgggcg agaaagctca ggatggcctg gcgaacaaac cggaacatca ccgcttatgg
    30541 gcgcaggcga tgccatcgcc agtcggtgat gacgggcttt atcaccatcc gctctatttt
    30601 gcctgtaatg actttgttga tgaccaaatc ggacgggtca tcaatgcctt aacgccagag
    30661 caacgtgaaa atacgtgggt catttatact tccgatcacg gcgaaatgat gggcgcacat
    30721 aagctgatca gtaaaggagc ggcgatgtat gacgacatca cccgtattcc gctgatcatc
    30781 cgttcgccgc aaggggagcg gcgacaggtc gatacgccag tcagtcatat cgatttactg
    30841 ccgacaatga tggcgctggc agatattgaa aaaccagaga ttctgccggg ggaaaatatc
    30901 cttgccgtga aagagccacg cggtgtaatg gtggaattta accgctacga gattgagcat
    30961 gacagctttg gcggttttat tccggtgcgt tgctgggtga cggatgactt taaactggta
    31021 ctcaacctct tcaccagtga tgaactttac gatcgccgta atgacccaaa tgaaatgcat
    31081 aacctgatcg atgatatccg ttttgcagac gttcgcagca aaatgcatga cgccttattg
    31141 gattacatgg acaaaattcg cgatccgttc cgcagttacc aatggagcct gcgtccgtgg
    31201 cgtaaagatg cactgccgcg ctggatgggg gcatttcgtc cacgcccaca agatggctat
    31261 tcgccggtgg tacgtgacta tgacaccggc ctaccgacgc aaggagtgaa agtggaagag
    31321 aaaaaacaga agttctgacg ataatgcccg ctgatgaaca cccggcgggc attattcagg
    31381 ctgcttaaac cagtgaaccg ccggtcagga aggaaaaaat caggaatatc agcaaaatgc
    31441 tgcacacgat gccgatacca aaaagcaagg tatggaaagc cagcgtcccg ccgccaaaaa
    31501 ccagcgcgcc gcgaatgccc cagcgatttt ccttacgcat accgccaata aatgcgcaaa
    31561 gtagcgcagc aatggccagt gcgataccgc tttgatcgag gattttatcg acatctacgt
    31621 cctctttttt ctccacggtg gtaatttttt cgccttttat tccggcgaga agacctcttt
    31681 taatttcagc cgttttgtcc gccacgatgc tttcaaacgt tggcggtggt gcggaaaaag
    31741 ggccgaatga aaagtggata atccccatca ttaaagcgat ggcaccgaac agcatgccca
    31801 atgaactgat tttattttga gctatgatac ccatctgacc tccttgtagc ggatggcgaa
    31861 agcatatcag gaaacgggcg gacgaaattc ttaaaaaaaa accgctaaag gtagagcgtt
    31921 agtgaaattg caaaggagca acaatgaaaa tttcccgcct tggagaagcg cccgattacc
    31981 gcttctcgct ggctaatgag cgtacttttc tggcgtggat ccgtaccgca ctaggatttc
    32041 tggcggcggg ggttgggctt gatcaacttg cgccagattt cgccacgccc gtcattcgtg
    32101 aactgctggc gttgttgttg tgtctgtttt ccggcgggtt agcgatgtat ggctacctgc
    32161 gctggttacg caatgaaaaa gcgatgcgtc tgaaagagga cctgccctac accaacagct
    32221 tattaatcat tagcttaata ttgatggtcg tggcggtgat cgttatggga ctggtgttgt
    32281 atgccggata gccgcaaagc cagacgcatt gccgacccag gactgcagcc ggagcgcaca
    32341 tcactggcgt ggtttcgtac catgctgggc tacggcgcgt tgatggcgtt ggctatcaaa
    32401 cacaactggc accaggcggg catgttattc tggatttcga ttggcattct cgccatcgtg
    32461 gcgctgatcc tctggcacta cacccgtaat cgcaatttaa tggatgtcac gaatagcgat
    32521 ttttcccaat ttcacgtagt ccgtgacaaa tttttgatct ccctcgcggt gttatctctc
    32581 gcaatactgt ttgctgtaac gcatatacat caacttatcg tatttattga gagagtcgca
    32641 tgacaggaag tcaggtcatt gatgctgaag aggacaggca taaactggtt gtagagtaca
    32701 aggatgctct gcaacctgcc gatttttatc acaatttcaa acagcggggc atccgctccg
    32761 tacagctcat tccctatctt gaatttgatg accggggcga tctgacggcc gcttcggtga
    32821 cagcagagct gtgggggaaa tttttaattg ccctgttcga gtgttgggtg cgggcagata
    32881 tcagtcgtat ctcgattgag ctttttgacg ccaccttaca aaaatggtgt ggcagcgaaa
    32941 acccgcaacc gcgccgtgac tgccaggcgt gcgactggca tcgcctgtgc ccgcacgctc
    33001 gccaggagac gccagacagc gtgctttgtg cgggctatca ggcgttttat tcttactccg
    33061 caccgcatat gcgggtgatg cgtgatctga taaagcagca tcgttctccc atggagctga
    33121 tgacgatgct gcggtgacgt gcgcttaaac gggctgcccc tgatgcgaca tccgcgtcgc
    33181 cagcggcagc cagcacagca cgatcaacaa tcccattaag gtcatcaaca accccaggct
    33241 gccctgaccg gtttgcggca acatcgcaga gagcgacgcc agcacgccgg aaccaatgtt
    33301 ttgcagaccg ccgaccagcg cgccagccgt gcccgccagg aaggggaacg gctccatcgc
    33361 gccgctggtc gccagcggaa acagcatccc ggcaccgaaa aagaacagcg cggcgggaac
    33421 gagcagcgtc cagacattca tcacgccaaa ccagtcgggg atccacatca gcaagccagc
    33481 cagcaggcag cagataaccg actgccacat taacgtggag aagcgtttat tgggacgtcc
    33541 ggcaaaccat gcgccaaaaa acgctgccgg aatcggcaga ataaacaaaa tactgaccgt
    33601 catactgctc agccctaaca ccgcgcccat cagcacgccg gagcaggctt caaaggcggc
    33661 aatcccggcc agaccgccaa tcagcatcag caaataacag ttaaaaccgc tgttaccgaa
    33721 aagcgtttta taactggtaa gcaggcgcgt gcgcggtgca tcgaccggac gcgtttccgg
    33781 catccagcgg gccatactga aggtcacacc agcacaaaga accaacaaaa agagataaca
    33841 ggcgcgccag ttccacatcg tatccagcag accgccgatt agcggtgcga gcaacggact
    33901 gacgagaatc cccatgttta acaggctgtt agcatggcgc aactgtgtcc gttcatataa
    33961 atctcgcggc aaagtacgcg ccattacgcc gccaacgccg gtgcccatcc cctgcatcgc
    34021 gctggcggca atcaacaccg tcaaactgga ggtcgtgacc gcgaccagcg ttgccagcat
    34081 aaaaatggac attccgacga ggatcaccgg tcggcggccc acgcggtcgg aaatcgggcc
    34141 ataaaacagc tgtgagacac cgtaagtcag cagataagcg cccattacgc tctgcaccgc
    34201 cccttcacgg acgttgagat cgcgcgccat atcggcaata gctggaatat aaatggtttg
    34261 cgccatctga ccgacggcca cgagtaatac caacatcaat aacaaattga cgtttctttg
    34321 ccttttcatt atcacggatg cttttataaa aataattaga ataagtcact acgcattccc
    34381 ataaatgcgg gagaggaatc tatcacattg atgaatgtta gccagatata cgccaggaat
    34441 ggggaattgt ttagcggcag aatatgtaaa caaaagcggc aataaatgtt gccgggaggg
    34501 attacgtgcg aactgtaacc ctctccccaa aggggcgagg gggaaaccac cgttcccttt
    34561 cagaaaatga gctaaacatt gcacgggacg gtcccctcgc ccctttgggg agagggctag
    34621 ggtgaggggc acaggcagcg caggtgcaaa cattctgctg gtagaagaag cccccaactc
    34681 caacccgcac gctaaatata aaaggggagc ggttacccgc tcccctttgg tgcgacttga
    34741 atctgaatta cttcaggtat ttcagaacag catcaagcag ttgcagtgct gcaacaatga
    34801 gtttgaggat aagaatggcg atatccacca ggctcatacg cgtctcctgt ggttcaggag
    34861 cactgccagc tgacgtacct ttccgctgcc tgttgccagc tgttgtgcac gcggtatctt
    34921 aaggagaaac gttgtgcttg ttgacataac acagtgtgct cacgcggggt taaggcgctg
    34981 acggcaccac ccgtttcagc caggacttag tgcgccgggt aacgatgcgg cccccgcata
    35041 acacattgcg tacagtgata ttatagaagt ttactgtata aataaacagt aatatttgga
    35101 caaaacgcaa actgtgatcg atactcaact atgccctaaa tacgtcaaaa ttcgtgccga
    35161 aattgcgcgt tctgcgcgga acacgtatac tttcagtgtt gacataatac agtgtgcttt
    35221 gcggttacca gccgcaggcg gctgatgaaa cctcgctccg gcggggtttt ttgttacctg
    35281 caattcagta caaaacgtga tcagcccctc aattttccct tgctgaaaaa ttttccattg
    35341 tctcccctgt aaagctgtgc ttgtataaat attgttaaac acaaaaccaa caaggtcccc
    35401 aatgactact tccatgttca acgcaaaact actaccaact gcgctatccg ccgcagtggt
    35461 cgtcgtgcgt gtggtggtgg tcgtcggcaa tgcgccgtag ggactggaac aacacacgat
    35521 tccaaaaccc cgccggcgca aaccgggcgg ggtttttcgt ttaagcacct cccggaaagt
    35581 cggcccagaa gaaaaggact ggagcatggc aagttcgggc acaacatcga cgcgtaagcg
    35641 ctttaccggc gcagaattta tcgttcattt cctggaacag cagggcatta agattgtgac
    35701 gggcattccg ggcggttcta tcctgcctgt ttacgatgcc ttaagccaaa gcacgcaaat
    35761 ccgccatatt ctggcccgtc atgaacaggg cgcgggattt atcgctcagg ggatggcgcg
    35821 caccgacggt aaaccggcgg tctgtatggc ctgtagcgga ccgggtgcga ctaacctggt
    35881 gaccgccatt gccgatgcgc ggctggactc catcccgctg atttgcatca ccggtcaggt
    35941 tcccgcctcg atgatcggca ccgacgtctt ccaggaagtg gacacctacg gcatctctat
    36001 ccccatcacc aaacacaact atctggtcag acatatcgaa gaactcccgc aggtcatgag
    36061 cgatgccttc cgcattgcgc aatcaggccg cccaggcccg gtgtggatag acattcctaa
    36121 ggatgtgcaa acggcagttt ttgagattga aacacagccc gctatggcag aaaaagccgc
    36181 cgcccccgcc tttagcgaag aaagcattcg tgacgcagcg gcgatgatta acgctgccaa
    36241 acgcccggtg ctttatctgg gcggcggtgt gatcaatgcg cccgcacggg tgcgtgaact
    36301 ggcggagaaa gcgcaactgc ctaccaccat gactttaatg gcgctgggca tgttgccaaa
    36361 agcgcatccg ttgtcgctgg gtatgctggg gatgcacggc gtgcgcagca ccaactatat
    36421 tttgcaggag gcggatttgt tgatagtgct cggtgcgcgt tttgatgacc gggcgattgg
    36481 caaaaccgag cagttctgtc cgaatgccaa aattattcat gtcgatatcg atcgcgcaga
    36541 gctgggtaaa atcaagcagc cgcatgtggc gattcaggcg gatgttgatg acgtgctggc
    36601 gcaattgatc ccgcaggtgg aagcgcaacc gcgtgcagag tggcaccagt tggtagcgga
    36661 tttgcagcgc gaattcccgt gtccaatccc gaaagcgtgc gatccattaa gccattacgg
    36721 cctgatcaac gccgttgccg cctgtgtcga tgacaatgcg attatcacca ccgatgtggg
    36781 tcagcatcag atgtggaccg cgcaagctta tccgctcaat cgcccacgcc agtggctgac
    36841 ctccggtggg ctgggcacga tgggttttgg cctgcctgcg gcgattggcg cggcgctggc
    36901 gaacccggat cgcaaagtgt tgtgtttctc cggcgacggc agcctgatga tgaatattca
    36961 ggagatggcg accgccagtg aaaatcagct ggatgtcaaa atcattctga tgaacaacga
    37021 agcgctgggg ctggtgcatc agcaacagag tctgttctac gagcaaggcg tttttgccgc
    37081 cacctatccg ggaaaaatca actttatgca gattgccgcc ggattcggcc tcgaaacctg
    37141 tgatttgaat aacgaagccg atccgcaggc tgcattgcag gaaatcatca atcgccctgg
    37201 cccggcgctg atccatgtgc gcattgatgc cgaagaaaaa gtttacccga tggtgccgcc
    37261 aggtgcggcg aatactgaaa tggtggggga ataagccatg caaaacacaa ctcatgacaa
    37321 cgtgattctg gagctcaccg ttcgcaacca tccgggcgta atgacccacg tttgtggcct
    37381 ttttgcccgc cgcgctttta acgttgaagg cattctttgt ctgccgattc aggacagcga
    37441 caaaagccat atctggctac tggtcaatga cgaccagcgt ctggagcaga tgataagcca
    37501 aatcgataag ctggaagatg tggtgaaagt gcagcgtaat cagtccgatc cgactatgtt
    37561 taacaagatt gcggtgtttt ttcagtaacc gctcaaggct tgaacaacat cgcgcttatc
    37621 gttaaggtaa gcgcgttttt ttttacccgc caggacaaga ccatgatcac cgttgccctt
    37681 atagacgatc acctcatcgt ccgctccggc tttgcgcagc tgctggggct ggaacctgat
    37741 ttgcaggtag ttgccgagtt tggttcgggg cgcgaggcgc tggcggggct gccggggcgc
    37801 ggtgtgcagg tgtgtatttg cgatatctcc atgcccgata tctccggtct ggaactgcta
    37861 agccagctgc cgaaaggtat ggcgacaata atgctctctg ttcatgacag tccggcgctg
    37921 gttgagcagg cgcttaacgc gggggcacgc ggctttctct ccaaacgctg tagccctgac
    37981 gaactgattg cggcggtgca tacggttgcc acgggcggct gttatctgac gccggatatt
    38041 gccattaaac tggcatccgg tcgtcaggac ccgctaacca aacgcgaacg gcaggtggcg
    38101 gaaaaactgg cgcaaggaat ggcggtgaaa gagattgctg ccgaactggg cttgtcgccg
    38161 aaaacggtgc acgtccatcg cgccaacctg atggaaaaac tgggtgtcag taacgacgta
    38221 gagctggcgc gccgcatgtt tgatggctgg tgatgaagac gttgttctcc cgcttaatta
    38281 ccgttattgc ctgctttttt atcttctctg ccgcatggtt ttgcctgtgg agtatcagcc
    38341 tgcacctggt tgagcgccct gatatggcgg tgctgttatt tccgtttggt ctgcgtctgg
    38401 ggctaatgct gcaatgcccg cgcggatact ggccggtgct gctgggcgcg gagtggctgc
    38461 tgatttactg gctaacgcag gcggtcggtt taacccattt tccgttattg atgatcggta
    38521 gtttactgac gttactgccc gtggcgctga tctcgcgcta tcgccatcag cgtgactggc
    38581 gcaccttgct gttacagggt gcggcgttaa cggcggcggc gttgttgcag tcgctgccct
    38641 ggctttggca cggcaaagag tcgtggaatg cgctgttgct gactttaact ggcggcctga
    38701 cgctggcccc gatatgtctg gtgttctggc actatctcgc caataacacc tggctgccgc
    38761 tcggcccgtc actggtttct cagccgatca actggcgcgg gcgacatctg gtctggtact
    38821 tgctgctgtt tgttatcagt ctctggctcc agttgggatt gccggacgaa ctgtcgcgct
    38881 ttacgccatt ttgtctggcg ctgccgatta tcgcgctggc ctggcactat ggttggcaag
    38941 gggcgctgat tgcgacgttg atgaacgcca tcgcgctgat cgccagtcaa acctggcgcg
    39001 atcatccggt ggatttattg ctctcgctgc tggtgcaaag tctgacaggg ttgttgcttg
    39061 gcgctggcat ccagcggttg cgtgaactta accagtcgct gcaaaaggaa ctggcgcgca
    39121 atcagcatct ggctgaacgg ttgctggaaa ccgaagagag cgtgcgccgt gatgtggcgc
    39181 gtgagctgca tgatgatatc ggtcagacca tcacagccat tcgtactcag gcgggcattg
    39241 ttcagcggct ggcggcagat aacgccagcg tgaagcagag cgggcagctc atcgaacaac
    39301 tatcgctggg cgtttacgac gcggtgcgcc gtttgttggg tcggttacgt ccgcgccagt
    39361 tggatgatct caccctggag caggccatcc gctcactgat gcgggaaatg gagctggaag
    39421 ggcgcggtat tgtcagccat ctcgaatggc gaatcgatga atcagcgtta agcgaaaacc
    39481 agcgcgtgac gctgtttcgt gtctgccagg aagggctgaa caacattgtg aaacatgctg
    39541 atgccagcgc ggtcaccctg caaggctggc agcaggatga acggttgatg ctggttattg
    39601 aagacgatgg cagcggtttg ccgccgggtt ccgggcaaca aggttttggc ctcaccggaa
    39661 tgcgcgagcg cgtaacggcg ctgggtggca cattacacat ttcctgtctg cacggcacgc
    39721 gtgtcagcgt ttctctacct caacgctatg tctaaggttt gatgatgttg ccgtttctga
    39781 aagcgcctgc cgatgcgcca ttaatgactg ataaatatga aattgatgcc cgctatcgct
    39841 actggcgtcg gcatattctg ctgaccatct ggctgggtta cgcgctgttt tacttcacgc
    39901 ggaaaagttt taacgccgcg gtaccagaaa tccttgctaa cggcgtgctc agccgtagcg
    39961 atatcggcct gttagcgacc ctgttttaca ttacttacgg cgtgtcgaag tttgtctccg
    40021 gcattgtcag cgatcgctca aatgcccgtt attttatggg gatagggctt atcgccacgg
    40081 gcatcatgaa cattctgttt ggcttctcga cgtcgctatg ggcgtttgcc gtgctctggg
    40141 tgctgaacgc ctttttccag ggctggggtt caccggtgtg tgcgcgtctg ttaacggcct
    40201 ggtattcacg taccgagcgc ggcggttggt gggcattatg gaacacggcg cataacgtcg
    40261 gcggcgcact cattcccatt gtgatggcag cgagcgcgct gcattacggc tggcgtgccg
    40321 ggatgatgat tgctggttgt atggcgatag tcgtggggat ttttctctgc tggcggctac
    40381 gcgatcgccc gcaggcgtta ggtttaccgg cggtcggtga atggcggcac gacgcgctgg
    40441 aaattgctca acaacaagaa ggggcagggt tgacgcgtaa agagatcctg accaaatatg
    40501 tgttgctgaa tccgtatatc tggctgcttt cgttttgcta tgtgctggtc tatgtggtcc
    40561 gggcggcaat caacgactgg ggcaatttgt atatgtccga gacgctgggc gtcgatctgg
    40621 tcacggcgaa tacggcagtg acgatgtttg aactgggcgg atttatcggt gcgctggtag
    40681 ccggttgggg ctcggacaaa ttgtttaacg gcaaccgtgg gccgatgaat ttgattttcg
    40741 ccgccggaat tttgctttca gtcggctcgc tgtggctgat gccatttgcc agctacgtga
    40801 tgcaggcaac ctgcttcttc accattggtt tttttgtctt tggcccgcag atgttaatcg
    40861 gcatggcggc ggcagagtgt tcccacaaag aggctgcagg ggcggcgacg gggtttgtcg
    40921 gcttgtttgc ttatctgggg gcgtcgcttg ctggttggcc gctggcgaaa gtactcgata
    40981 cctggcactg gagcggattt tttgtggtta tctctatcgc cgccggggtt tccgcactgc
    41041 tgttactgcc ctttttgaac gcccaggcac cgcgcgaagc gtgatgcatc tcaccttttc
    41101 acttcatatc cggcaaaact aagaaatttt ccaggttttg cctggacgct atctcaggcc
    41161 tgatttgctg ctgattttta caatgccatc attcattaat tctttcccaa aataattcaa
    41221 gttgcaggaa cgcagcaaac gcgtgagtcc cgggagcata gtgaactatg tgaccgggag
    41281 gaatgcgtgc agctaaggca cctgcggctt gaagtatgaa gggaaatcag gagtaaccca
    41341 tgctggcttt cttaaaccag gttcgcaagc cgaccctgga ccttccgctc gaagtgcggc
    41401 gcaaaatgtg gttcaaaccg ttcatgcaat cctacctggt ggtctttatc ggctacctga
    41461 cgatgtacct gattcgcaag aactttaaca tcgcgcagaa cgacatgatc tcgacctacg
    41521 ggttgagcat gacgcagctg gggatgatcg gcctgggttt ctccatcact tatggcgtgg
    41581 gtaaaacgct ggtttcctac tacgccgacg gcaaaaacac caaacaattc ctgccgttca
    41641 tgctgatcct gtctgccatt tgtatgctgg gcttcagtgc cagtatgggc agcggctcgg
    41701 ttagcctgtt cctgatgatt gccttctacg ccttaagcgg ctttttccag agtaccggcg
    41761 gttcgtgcag ttactccacc atcaccaaat ggacgccgcg tcgtaaacgc gggacattcc
    41821 tcggtttctg gaatatttct cacaaccttg gcggtgcagg cgcagcaggt gtggcgctgt
    41881 tcggggcaaa ttacctgttc gatggccatg tcatcggcat gtttatcttc ccgtcgatta
    41941 tcgcgctgat tgtcggtttt atcggcctgc gttacggcag cgactccccg gaatcttatg
    42001 gcctcggcaa agctgaagaa ctgttcggcg aggagatcag cgaagaggac aaagagacag
    42061 aatctaccga tatgaccaag tggcagatct ttgttgagta tgtgctgaaa aacaaagtga
    42121 tctggctgct gtgcttcgcc aacattttcc tctatgtggt acgtattggt atcgaccagt
    42181 ggtcaaccgt atacgcgttc caggaactga aactctctaa agcggtggcg attcagggct
    42241 ttacgctgtt tgaagctggt gcactggtcg gtacgctgct gtggggctgg ctctctgacc
    42301 tggcgaacgg tcgccgtggc ctggtggcct gcatcgcgct ggcgctgatt atcgccacgc
    42361 tcggtgtgta tcaacatgcc agcaacgaat atatctatct ggcttctctc tttgcgttgg
    42421 gtttcctggt ctttggcccg caattgttga ttggtgtggc tgctgttggc tttgtaccta
    42481 aaaaagcgat tggcgctgcc gatggtatta aaggcacctt tgcttacctg attggtgaca
    42541 gctttgccaa gttaggtctg ggaatgattg ccgatgggac gccggtattc ggccttaccg
    42601 gctgggcagg caccttcgcc gcacttgata tcgccgcgat tggttgtatc tgcctgatgg
    42661 cgatagtggc ggtgatggaa gaacgcaaaa tccgccgcga gaaaaaaatt cagcagttga
    42721 cagtggcata aacgtaactg gtgacttttg cccggcatga cgccgggctt ttttattatt
    42781 ccgtgacttc cagcgtagtg aaggcaaact tctcgccatc aaatagcccc tgactggtta
    42841 gttttagcgc ggggatcact ggcagagaaa gaaacgccat ctgaataaac ggctcatcgg
    42901 gtaacggacc gcattcacgg gcggcggctt tcaaggcgtc aatttgttcc gccagcgact
    42961 gcgccgtgtc ggtgctcatc aggccggcaa tgggcaacgg cagatgactt tggacctggc
    43021 cgttacgtac cacgcacagc ccgccgccat cctgaatcac ctgattgacc gccagcgcca
    43081 tctcttcggc actgcgaccg atcaccacaa tattatggct gtcatggctg accgtcgcag
    43141 ccagcgcacc ttcattcagt ccaaagccgc caagcaaacc acaagccgga gccagccgtt
    43201 gcccgtaacg ctcaagtacg gcaataaagc tcacatcatc gcgatcaaaa ccattttcgc
    43261 tgtagacgct ggagtgggag tgcgtaatca attcgttatg gatgacgtca atgacccgat
    43321 agcgttttcc gggcgtaaat tgcagggcaa agtcgctggc ggaaactggc tggcgggcaa
    43381 tggtgttgcc atatggcgga gcggattgtg ccagtctcgc cgactcttcc gcctgtaagg
    43441 tttgcgcatc aatcggctcg cctttcacca gtacctgctg caccgtgacc ttacgcgcat
    43501 cgctcaacag gacgatatcg gcctgcttgc cgggtgccag taagccgagg tgattcagac
    43561 caaagtggcg cgccgtcgac cagctggcga cgcgatatgc cacatgcagc ggcacattgt
    43621 gttgttcgat caggcggcga attaaggcat cgatgtgtcc ttcatgggcg atctcccacg
    43681 ggttacggtc atcggtacag agcatgcatt gcgggctgtt aaattcgttg atcaacggtg
    43741 ccagcgcgtt gagattgcgg gcagcggacc cttcgcggat catcaacgac atgccgagtt
    43801 gtaatttccg gcgtccttct tccagctgat aactttcgtg gcagttttca atacccgcag
    43861 taatataggc gttaagttct ttaccaccca aacccgggca gtgaccgtcc agcgtcaggt
    43921 ggcgaaatgc atccagttta tcgagcagcg cattctgccc gctaattacg ccagggtagt
    43981 ccatcatttc tgcaaggccg gtaacctgcg gatggtcccg ccaggcgagc atctgttcaa
    44041 gggtaaaact ggcaccgtta acatcgcagc cttccagggc gggtacgcaa gagctgacct
    44101 gtaagtactg gttttgcctt gcctgttcgg cacagcgggc aaaccaggcg aatccggctt
    44161 ctcccatcac gttgacgatt tcatgagggt cgcaaataac ggtcgtcagg ccgcgcggca
    44221 gggtagcggt ttcaaaagtg accggcgtca tcatgctgga ttcaatatgc aggtgagcat
    44281 caataaaccc tggcaccgcc gttgcgccgc gagcatcaat ccgctgcaaa gccggagcat
    44341 cagcgtattc tgcgccaaca ccggcaatgt aacgtccttt aatcacaatt gggccggaaa
    44401 tttctccgcc attgatcagg tcgagaatag agacattatc aataatataa tcggcaacag
    44461 cgtcgccacg ggaaacggct aacaa
//
LOCUS       DAZKHF020000010        42959 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2201S31241
            SAMN43267800-rid25106413.denovo.010, whole genome shotgun sequence.
ACCESSION   DAZKHF020000010 DAZKHF020000000
VERSION     DAZKHF020000010.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267800
            Sequence Read Archive: SRR30307263
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 42959)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 42959)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 42959)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350581.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350581.2
            Genome Coverage       :: 151x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:21:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,652
            CDSs (total)                      :: 5,557
            Genes (coding)                    :: 5,320
            CDSs (with protein)               :: 5,320
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 237
            CDSs (without protein)            :: 237
            Pseudo Genes (ambiguous residues) :: 0 of 237
            Pseudo Genes (frameshifted)       :: 102 of 237
            Pseudo Genes (incomplete)         :: 130 of 237
            Pseudo Genes (internal stop)      :: 52 of 237
            Pseudo Genes (multiple problems)  :: 41 of 237
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..42959
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267800-rid25106413.denovo.010"
                     /serotype="O 26:H11"
                     /isolate="2201S31241"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-01"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            complement(<1..77)
                     /gene="hokE"
                     /locus_tag="ACHOSC_RS02150"
                     /old_locus_tag="pgaptmp_000430"
     CDS             complement(<1..77)
                     /gene="hokE"
                     /locus_tag="ACHOSC_RS02150"
                     /old_locus_tag="pgaptmp_000430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_025295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin HokE"
                     /protein_id="HGU4546442.1"
                     /translation="MLTKYALAAVIVLCLTVLGFTLLVG"
     gene            46..315
                     /locus_tag="ACHOSC_RS02155"
                     /old_locus_tag="pgaptmp_000431"
     CDS             46..315
                     /locus_tag="ACHOSC_RS02155"
                     /old_locus_tag="pgaptmp_000431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009008364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4546443.1"
                     /translation="MTAARAYFVSMFILPPCEEGTTIQIVQVWVGSPRVDRNIVLGLH
                     LSRPQSKPETGKLQAPAVILPLLSKIAVYLYSKSCFGLLTPHLIN"
     gene            519..1637
                     /gene="ybdK"
                     /locus_tag="ACHOSC_RS02160"
                     /old_locus_tag="pgaptmp_000432"
     CDS             519..1637
                     /gene="ybdK"
                     /locus_tag="ACHOSC_RS02160"
                     /old_locus_tag="pgaptmp_000432"
                     /EC_number="6.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004357 - glutamate-cysteine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0042398 - cellular modified amino acid
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YbdK family carboxylate-amine ligase"
                     /protein_id="HGU4546444.1"
                     /translation="MPLPDFHVSEPFTLGIELEMQVVNPPGYDLSQDSSMLIDAVKNE
                     ITAGEVKHDITESMLELATDVCRDINQAAGQFSAMQKVVLQAAADHHLEICGGGTHPF
                     QKWQRQEVCDNERYQRTLENFGYLIQQATVFGQHVHVGCASGDDAIYLLHGLSRFVPH
                     FIALSAASPYMQGTDTRFASSRPNIFSAFPDNGPMPWVSNWQQFEALFRCLSYTTMID
                     SIKDLHWDIRPSPHFGTVEVRVMDTPLTLSHAVNMAGLIQATAHWLLTERPFKHQEKD
                     YLLYKFNRFQACRYGLEGVITDPHTGDRRPLTEDTLRLLEKIAPSAHKMGASSAIEAL
                     HRQVVSGLNEAQLMRDFVADGGSLIGLVKKHCEIWAGD"
     gene            1703..1951
                     /gene="ybdJ"
                     /locus_tag="ACHOSC_RS02165"
                     /old_locus_tag="pgaptmp_000433"
     CDS             1703..1951
                     /gene="ybdJ"
                     /locus_tag="ACHOSC_RS02165"
                     /old_locus_tag="pgaptmp_000433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1158 domain-containing protein"
                     /protein_id="HGU4546445.1"
                     /translation="MKHPLETLTTAAGILLMAFLSCLLLPAPALGLTLAQKLVTMFHL
                     MDLSQLYTLLFCLWFLVLGAIEYFVLRFIWQRWFSLAD"
     gene            2016..2384
                     /gene="ybdF"
                     /locus_tag="ACHOSC_RS02170"
                     /old_locus_tag="pgaptmp_000434"
     CDS             2016..2384
                     /gene="ybdF"
                     /locus_tag="ACHOSC_RS02170"
                     /old_locus_tag="pgaptmp_000434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MmcQ/YjbR family DNA-binding protein"
                     /protein_id="HGU4546446.1"
                     /translation="MDKQSLHETAKRLALELPFVELCWPFGPEFDVFKIGGKIFMLSS
                     ELRGVPFINLKSDPQKSLLNQQIYPSIKPGYHMNKKHWISVYPGEEISEALLRDLIND
                     SWNLVVDGLAKRDQKRVRPG"
     gene            2478..3131
                     /gene="nfsB"
                     /locus_tag="ACHOSC_RS02175"
                     /old_locus_tag="pgaptmp_000435"
     CDS             2478..3131
                     /gene="nfsB"
                     /locus_tag="ACHOSC_RS02175"
                     /old_locus_tag="pgaptmp_000435"
                     /EC_number="1.5.1.34"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oxygen-insensitive NAD(P)H nitroreductase"
                     /protein_id="HGU4546447.1"
                     /translation="MDIISVALKRHSTKAFDASKKLTPEQAEQIKTLLQYSPSSTNSQ
                     PWHFIVASTEEGKARVAKSAAGNYVFNERKILDASHVVVFCAKTAMDDAWLKLVVDQE
                     DADGRFATPEAKAANDKGRKFFADMHRKDLHDDAEWMAKQVYLNVGNFLLGVAALGLD
                     AVPIEGFDAAILDAEFGLKEKGYTSLVVVPVGHHSVEDFNATLPKSRLPQNITLTEV"
     gene            3239..4486
                     /gene="ybdG"
                     /locus_tag="ACHOSC_RS02180"
                     /old_locus_tag="pgaptmp_000436"
     CDS             3239..4486
                     /gene="ybdG"
                     /locus_tag="ACHOSC_RS02180"
                     /old_locus_tag="pgaptmp_000436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel YbdG"
                     /protein_id="HGU4546448.1"
                     /translation="MQDLISQVEDLAGIEIDHTTSMVMIFGIIFLTAVVVHIILHWVV
                     LRTFEKRAIASSRLWLQIITQNKLFHRLAFTLQGIIVNIQAVFWLQKGTEAADILTTC
                     AQLWIMMYALLSVFSLLDVILNLAQKFPAASQLPLKGIFQGIKLIGAILVGILMISLL
                     IGQSPAILISGLGAMAAVLMLVFKDPILGLVAGIQLSANDMLKLGDWLEMPKYGADGA
                     VIDIGLTTVKVRNWDNTITTIPTWSLVSDSFKNWSGMSASGGRRIKRSISIDVTSIRF
                     LDEDEMQRLNKAHLLKPYLTSRHQEINEWNRQQGSTESILNLRRMTNIGTFRAYLNEY
                     LRNHPRIRKDMTLMVRQLAPGDNGLPLEIYAFTNTVVWLEYESIQADIFDHIFAIVEE
                     FGLRLHQSPTGNDIRSLAGAFKQ"
     gene            complement(4554..5930)
                     /gene="pheP"
                     /locus_tag="ACHOSC_RS02185"
                     /old_locus_tag="pgaptmp_000437"
     CDS             complement(4554..5930)
                     /gene="pheP"
                     /locus_tag="ACHOSC_RS02185"
                     /old_locus_tag="pgaptmp_000437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine transporter"
                     /protein_id="HGU4546449.1"
                     /translation="MKNASTVSEDTASNQEPTLHRGLHNRHIQLIALGGAIGTGLFLG
                     IGPAIQMAGPAVLLGYGVAGIIAFLIMRQLGEMVVEEPVSGSFAHFAYKYWGPFAGFL
                     SGWNYWVMFVLVGMAELTAAGIYMQYWFPDVPTWIWAAAFFIIINAVNLVNVRLYGET
                     EFWFALIKVLAIIGMIGFGLWLLFSGHGGEKASIDNLWRYGGFFATGWNGLILSLAVI
                     MFSFGGLELIGITAAEARDPEKSIPKAVNQVVYRILLFYIGSLVVLLALYPWVEVKSN
                     SSPFVMIFHNLDSNVVASALNFVILVASLSVYNSGVYSNSRMLFGLSVQGNAPKFLTR
                     VSRRGVPINSLMLSGAITSLVVLINYLLPQKAFGLLMALVVATLLLNWIMICLAHLRF
                     RAAMRRQGRETQFKALLYPFGNYLCIAFLGMILLLMCTMDDMRLSAILLPVWIVFLFV
                     AFKTLRRK"
     gene            complement(6032..9175)
                     /gene="cusA"
                     /locus_tag="ACHOSC_RS02190"
                     /old_locus_tag="pgaptmp_000438"
     CDS             complement(6032..9175)
                     /gene="cusA"
                     /locus_tag="ACHOSC_RS02190"
                     /old_locus_tag="pgaptmp_000438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cu(+)/Ag(+) efflux RND transporter permease
                     subunit CusA"
                     /protein_id="HGU4546450.1"
                     /translation="MIEWIIRRSVANRFLVLMGALFLSIWGTWTIINTPVDALPDLSD
                     VQVIIKTSYPGQAPQIVENQVTYPLTTTMLSVPGAKTVRGFSQFGDSYVYVIFEDGTD
                     PYWARSRVLEYLNQVQGKLPAGVSAELGPDATGVGWIYEYALVDRSGKHDLADLRSLQ
                     DWFLKYELKTIPDVAEVASVGGVVKEYQVVIDPQRLAQYGISLAEVKSALDASNQEAG
                     GSSIELAEAEYMVRASGYLQTLDDFNHIVLKASENGVPVYLRDVAKVQIGPEMRRGIA
                     ELNGEGEVAGGVVILRSGKNAREVIAAVKDKLETLKSSLPEGVEIVTTYDRSQLIDRA
                     IDNLSGKLLEEFIVVAVVCALFLWHVRSALVAIISLPLGLCIAFIVMHFQGLNANIMS
                     LGGIAIAVGAMVDAAIVMIENAHKRLEEWQHQHPDATLDNKTRWQVITDASVEVGPAL
                     FISLLIITLSFIPIFTLEGQEGRLFGPLAFTKTYAMAGAALLAIVVIPILMGYWIRGK
                     IPPESSNPLNRFLIRVYHPLLLKVLHWPKTTLLVAALSVLTVLWPLNKVGGEFLPQIN
                     EGDLLYMPSTLPGISAAEAASMLQKTDKLIMSVPEVARVFGKTGKAETATDSAPLEMV
                     ETTIQLKPQEQWRPGMTMDKIIEELDNTVRLPGLANLWVPPIRNRIDMLSTGIKSPIG
                     IKVSGTVLADIDAMAEQIEEVARTVPGVASALAERLEGGRYINVEINREKAARYGMTV
                     ADVQLFVTSAVGGAMVGETVEGIARYPINLRYPQSWRDSPQALRQLPILTPMKQQITL
                     ADVADIKVSTGPSMLKTENARPTSWIYIDARDRDMVSVVHDLQKAIAEKVQLKPGTSV
                     AFSGQFELLERANHKLKLMVPMTLMIIFVLLYLAFRRVGEALLIISSVPFALVGGIWL
                     LWWMGFHLSVATGTGFIALAGVAAEFGVVMLMYLRHAIEAEPSLNNPQTFSEQKLDEA
                     LYHGAVLRVRPKAMTVAVIIAGLLPILWGTGAGSEVMSRIAAPMIGGMITAPLLSLFI
                     IPAAYKLMWLHRHRVRK"
     gene            complement(9187..10410)
                     /gene="cusB"
                     /locus_tag="ACHOSC_RS02195"
                     /old_locus_tag="pgaptmp_000439"
     CDS             complement(9187..10410)
                     /gene="cusB"
                     /locus_tag="ACHOSC_RS02195"
                     /old_locus_tag="pgaptmp_000439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cu(+)/Ag(+) efflux RND transporter periplasmic
                     adaptor subunit CusB"
                     /protein_id="HGU4546451.1"
                     /translation="MKKIALIIGSMIAGGIISAAGFTWFAKAEPPAEKTSTAERKILF
                     WYDPMYPNTRFDKPGKSPFMDMDLVPKYADEESSASGVRIDPTQTQNLGVKTATVTRG
                     PLTFAQSFPANVSYNEYQYAIVQARAAGFIDKVYPLTVGDKVQKGTPLLDLTIPDWVE
                     AQSEYLLLRETGGTATQTEGILERLRLAGMPEADIRRLIATQKIQTRFTLKAPIDGVI
                     TAFDLRAGMNIAKDNVVAKIQGMDPVWVTAAIPESIAWLVKDASQFTLTVPARPDKTL
                     TIRKWTLLPGVDAATRTLQLRLEVDNADEALKPGMNAWLQLNTASEPMLLIPSQALID
                     TGSEQRVITVDADGRFVPKRVAVFQVSQGVTALRSGLAEGEKVVSSGLFLIDSEANIS
                     GALERMRSESATHAH"
     gene            complement(10426..10758)
                     /gene="cusF"
                     /locus_tag="ACHOSC_RS02200"
                     /old_locus_tag="pgaptmp_000440"
     CDS             complement(10426..10758)
                     /gene="cusF"
                     /locus_tag="ACHOSC_RS02200"
                     /old_locus_tag="pgaptmp_000440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cu(+)/Ag(+) efflux RND transporter periplasmic
                     metallochaperone CusF"
                     /protein_id="HGU4546452.1"
                     /translation="MKKALQVAMFSLFTVIGFNAQANEHHHETMSEVQPQVISATGVV
                     KGIDLESKKITIHHDPIAAVNWPEMTMRFTITPQTKMSEIKTGDKVAFNFVQQGNLSL
                     LQDIKVSQ"
     gene            complement(10911..12344)
                     /gene="ybhI"
                     /locus_tag="ACHOSC_RS02205"
                     /old_locus_tag="pgaptmp_000441"
     CDS             complement(10911..12344)
                     /gene="ybhI"
                     /locus_tag="ACHOSC_RS02205"
                     /old_locus_tag="pgaptmp_000441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anion permease"
                     /protein_id="HGU4546453.1"
                     /translation="MNKKSLWKLILILAIPCIIGFMPAPAGLSELAWVLFGIYLAAIV
                     GLVIKPFPEPVVLLIAVAASMVVVGNLSDGAFKTTAVLSGYSSGTTWLVFSAFTLSAA
                     FVTTGLGKRIAYLLIGKIGNTTLGLGYVTVFLDLVLAPATPSNTARAGGIVLPIINSV
                     AVALGSEPEKSPRRVGHYLMMSIYMVTKTTSYMFFTAMAGNILALKMINDILHLQISW
                     GGWALAAGLPGIIMLLVTPLVIYTMYPPEIKKVDNKTIAKAGLAELGPMKIREKMLLG
                     VFVLALLGWIFSKSLGVDESTVAIVVMATMLLLGIVTWEDVVKNKGGWNTLIWYGGII
                     GLSSLLSKVKFFEWLAEVFKNNLAFDGHGNVAFFVIIFLSIIVRYFFASGSAYIVAML
                     PVFAMLANVSGAPLMLTALALLFSNSYGGMVTHYGGAAGPVIFGVGYNDIKSWWLVGA
                     VLTILTFLVHITLGVWWWNMLIGWNML"
     gene            complement(12420..13472)
                     /gene="ybhH"
                     /locus_tag="ACHOSC_RS02210"
                     /old_locus_tag="pgaptmp_000442"
     CDS             complement(12420..13472)
                     /gene="ybhH"
                     /locus_tag="ACHOSC_RS02210"
                     /old_locus_tag="pgaptmp_000442"
                     /EC_number="5.3.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-oxalomesaconate tautomerase"
                     /protein_id="HGU4546454.1"
                     /translation="MKKIPCVMMRGGTSRGAFLLAEHLPEDQTQRDKILMAIMGSGND
                     LEIDGIGGGNPLTSKVAIISRSSDPRADVDYLFAQVIVHEQRVDTTPNCGNMLSGVGA
                     FAIENGLIAATSPVTRVRIRNVNTGTFIEADVQTPNGVVEYEGSARIDGVPGTAAPVA
                     LTFLNAAGTKTGKVFPTDNQIDYFDDVPVTCIDMAMPVVIIPAEYLGKTGYELPAELD
                     ADKALLARIESIRLQAGKAMGLGDVSNMVIPKPVLISPAQKGGAINVRYFMPHSCHRA
                     LAITGAIAISSSCALEGTVTRQIVPSVGYGNINIEHPSGALDVHLSNEGQDATTLRAS
                     VIRTTRKIFSGEVYLP"
     gene            13656..14609
                     /gene="ybhD"
                     /locus_tag="ACHOSC_RS02215"
                     /old_locus_tag="pgaptmp_000443"
     CDS             13656..14609
                     /gene="ybhD"
                     /locus_tag="ACHOSC_RS02215"
                     /old_locus_tag="pgaptmp_000443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308823.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HGU4546455.1"
                     /translation="MKHELSSMKAFVILAESSSFNNAAKLLNITQPALTRRIKKMEED
                     LHIQLFERTTRKVTLTKAGKRLLPEARELIKKFDETLFNIRDMNAYHRGMVTLACIPT
                     AVFYFLPLAIGKFNELYPNIKVRILEQGTNNCMESVLCNESDFGINMNNVTNSSIDFT
                     PLVNEPFVLACRRDHPLAKKQLVEWQELVGYKMIGVRSSSGNRLLIEQQLADKPWKLD
                     WFYEVRHLSTSLGLVEAGLGISALPGLAMPHAPYSSIIGIPLVEPVIRRTLGIIRRKD
                     AVLSPAAERFFALLINLWTDDKDNLWTNIVERQRHALQEIG"
     gene            complement(14650..15645)
                     /gene="pgl"
                     /locus_tag="ACHOSC_RS02220"
                     /old_locus_tag="pgaptmp_000444"
     CDS             complement(14650..15645)
                     /gene="pgl"
                     /locus_tag="ACHOSC_RS02220"
                     /old_locus_tag="pgaptmp_000444"
                     /EC_number="3.1.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0017057 - 6-phosphogluconolactonase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphogluconolactonase"
                     /protein_id="HGU4546456.1"
                     /translation="MKQTVYIASPESQQIHVWNLNHEGALTLTQVVDVPGQVQPMVVS
                     PDKRYLYVGVRPEFRVLAYSIAPDDGALTFAAESALPGSPTHISTDHQGQFVFVGSYN
                     AGNVSVTRLEDGLPVGVVDVVEGLDGCHSANISPDNRTLWVPALKQDRICLFTVSDDG
                     HLVAQDPAEVTTVEGAGPRHMVFHPNEQYAYCVNELNSSVDVWELKDPHGNIECVQTL
                     DMMPENFSDTRWAADIHITPDGRHLYACDRTASLITVFSVSEDGSVLSKEGFQPTETQ
                     PRGFNVDHSGKYLIAAGQKSHHISVYEIVGEQGLLHEKGRYAVGQGPMWVVVNAH"
     gene            15800..16618
                     /gene="ybhA"
                     /locus_tag="ACHOSC_RS02225"
                     /old_locus_tag="pgaptmp_000445"
     CDS             15800..16618
                     /gene="ybhA"
                     /locus_tag="ACHOSC_RS02225"
                     /old_locus_tag="pgaptmp_000445"
                     /EC_number="3.1.3.74"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional pyridoxal
                     phosphate/fructose-1,6-bisphosphate phosphatase"
                     /protein_id="HGU4546457.1"
                     /translation="MTTRVIALDLDGTLLTPKKTLLPSSIEALARAREAGYQLIIVTG
                     RHHVAIHPFYQALALDTPAICCNGTYLYDYHAKTVLEADPMPVNKALQLIEMLNEHHI
                     HGLMYVDDAMVYEHPTGHVIRTSNWAQTLPPEQRPTFTQVASLAETAQQVNAVWKFAL
                     THDDLPQLQHFGKHVEHELGLECEWSWHDQVDIARGGNSKGKRLTKWVEAQGWSMENV
                     VAFGDNFNDISMLEAAGTGVAMGNADDAVKARANIVIGDNTTDSIAQFIYSHLI"
     gene            complement(16619..17677)
                     /gene="modC"
                     /locus_tag="ACHOSC_RS02230"
                     /old_locus_tag="pgaptmp_000446"
     CDS             complement(16619..17677)
                     /gene="modC"
                     /locus_tag="ACHOSC_RS02230"
                     /old_locus_tag="pgaptmp_000446"
                     /EC_number="7.3.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015098 - molybdate ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015689 - molybdate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum ABC transporter ATP-binding protein
                     ModC"
                     /protein_id="HGU4546458.1"
                     /translation="MLELNFSQTLGNHCLTINETLPANGITAIFGVSGAGKTSLINAI
                     SGLTRPQKGRIVLNGRVLNDAEKGICLTPEKRRVGYVFQDARLFPHYKVRGNLRYGMA
                     KSMVDQFDKLVALLGIEPLLDRLPGSLSGGEKQRVAIGRALLTAPELLLLDEPLASLD
                     IPRKRELLPYLQRLTREINIPMLYVSHSLDEILHLADRVMVLENGQVKAFGALEEVWG
                     SSVMNPWLPKEQQSSILKVTVLEHHPHYAMTALALGDQHLWVNKLDEPLQAALRIRIQ
                     ASDVSLVLQPPQQTSIRNVLRAKVVNSYDDNGQVEVELEVGGKTLWARISPWARDELV
                     IKPGLWLYAQIKSVSITA"
     gene            complement(17680..18369)
                     /gene="modB"
                     /locus_tag="ACHOSC_RS02235"
                     /old_locus_tag="pgaptmp_000447"
     CDS             complement(17680..18369)
                     /gene="modB"
                     /locus_tag="ACHOSC_RS02235"
                     /old_locus_tag="pgaptmp_000447"
                     /EC_number="7.3.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015098 - molybdate ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015689 - molybdate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdate ABC transporter permease subunit"
                     /protein_id="HGU4546459.1"
                     /translation="MILTDPEWQAVLLSLKVSSLAVLFSLPFGIFFAWLLVRCTFPGK
                     ALLDSVLHLPLVLPPVVVGYLLLVSMGRRGFIGERLYDWFGITFAFSWRGAVLAAAVM
                     SFPLMVRAIRLALEGVDVKLEQAARTLGAGRWRVFFTITLPLTLPGIIVGTVLAFARS
                     LGEFGATITFVSNIPGETRTIPSAMYTLIQTPGGESGAARLCIISIALAMISLLISEW
                     LARISRERAGR"
     gene            complement(18369..19142)
                     /gene="modA"
                     /locus_tag="ACHOSC_RS02240"
                     /old_locus_tag="pgaptmp_000448"
     CDS             complement(18369..19142)
                     /gene="modA"
                     /locus_tag="ACHOSC_RS02240"
                     /old_locus_tag="pgaptmp_000448"
                     /EC_number="7.3.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015689 - molybdate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdate ABC transporter substrate-binding
                     protein"
                     /protein_id="HGU4546460.1"
                     /translation="MARKWLNLFAGAALSFAVAGNTLADEGKITVFAAASLTNAMQDI
                     ATQYKKEKGVDVVSSFASSSTLARQIEAGAPADLFISADQKWMDYAVDKKAIDTASRQ
                     TLLGNSLVVVAPKASEQKDFTIDSKTNWTSLLNGGRLAVGDPEHVPAGIYAKEALQKL
                     GAWDTLSPKLAPAEDVRGALALVERNEAPLGIVYGSDAVASKGVKVVATFPEDSHKKV
                     EYPVAVVEEHNNATVKAFYDYLKGPQAAEIFKRYGFTTK"
     gene            complement(19308..19457)
                     /gene="acrZ"
                     /locus_tag="ACHOSC_RS02245"
                     /old_locus_tag="pgaptmp_000449"
     CDS             complement(19308..19457)
                     /gene="acrZ"
                     /locus_tag="ACHOSC_RS02245"
                     /old_locus_tag="pgaptmp_000449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux pump accessory protein AcrZ"
                     /protein_id="HGU4546461.1"
                     /translation="MLELLKSLVFAVIMVPVVMAIILGLIYGLGEVFNIFSGVGKKDQ
                     PGQNH"
     gene            19586..20374
                     /gene="modE"
                     /locus_tag="ACHOSC_RS02250"
                     /old_locus_tag="pgaptmp_000450"
     CDS             19586..20374
                     /gene="modE"
                     /locus_tag="ACHOSC_RS02250"
                     /old_locus_tag="pgaptmp_000450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0030151 - molybdenum ion binding [Evidence
                     IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0015689 - molybdate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum-dependent transcriptional regulator"
                     /protein_id="HGU4546462.1"
                     /translation="MQAEILLTLKLQQKLFADPRRISLLKHIALSGSISQGAKDAGIS
                     YKSAWDAINEMNQLSEHILVERATGGKGGGGAVLTRYGQRLIQLYDLLAQIQQKAFDV
                     LSDDDALPLNSLLAAISRFSLQTSARNQWFGTITARDHDDVQQHVDVLLADGKTRLKV
                     AITAQSGARLGLDEGKEVLILLKAPWVGITQDEAVAQNADNQLPGIISHIERGAEQCE
                     VLMALPDGQTLCATVPVNEATSLQQGQNVTAYFNADSVIIATLC"
     gene            20442..21914
                     /gene="modF"
                     /locus_tag="ACHOSC_RS02255"
                     /old_locus_tag="pgaptmp_000451"
     CDS             20442..21914
                     /gene="modF"
                     /locus_tag="ACHOSC_RS02255"
                     /old_locus_tag="pgaptmp_000451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdate ABC transporter ATP-binding protein
                     ModF"
                     /protein_id="HGU4546463.1"
                     /translation="MSSLQILQGTFRLSDTKTLQLPQLTLNAGDSWAFVGSNGSGKSA
                     LARALAGELPLLKGERQSQFSHITRLSFEQLQKLVSDEWQRNNTDMLGPGEDDTGRTT
                     AEIIQDEVKDAPRCMQLAQQFGITALLDRRFKYLSTGETRKTLLCQALMSEPDLLILD
                     EPFDGLDVASRQQLAERLASLHQSGITLVLVLNRFDEIPEFVQFAGVLADCTLAETGA
                     KEELLQQALVAQLAHSEQLEGVQLPEPDEPSARHALPANEPRIVLNNGVVSYNDRPIL
                     NNLSWQVNPGEHWQIVGPNGAGKSTLLSLVTGDHPQGYSNDLTLFGRRRGSGETIWDI
                     KKHIGYVSSSLHLDYRVSTTVRNVILSGYFDSIGIYQAVSDRQQKLVQQWLDILGIDK
                     RTADAPFHSLSWGQQRLALIVRALVKHPTLLILDEPLQGLDPLNRQLIRRFVDVLISE
                     GETQLLFVSHHAEDAPACITHRLEFVPDGGLYRYVLTKIY"
     gene            22175..23191
                     /gene="galE"
                     /locus_tag="ACHOSC_RS02260"
                     /old_locus_tag="pgaptmp_000452"
     CDS             22175..23191
                     /gene="galE"
                     /locus_tag="ACHOSC_RS02260"
                     /old_locus_tag="pgaptmp_000452"
                     /EC_number="5.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415280.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003978 - UDP-glucose 4-epimerase activity
                     [Evidence IEA];
                     GO_process: GO:0006012 - galactose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose 4-epimerase GalE"
                     /protein_id="HGU4546464.1"
                     /translation="MRVLVTGGSGYIGSHTCVQLLQNGHDVIILDNLCNSKRSVLPVI
                     ERLGGKHPTFVEGDIRNEALMTEILHDHAIDTVIHFAGLKAVGESVQKPLEYYDNNVN
                     GTLRLISAMRAANVKNFIFSSSATVYGDQPKIPYVESFPTGTPQSPYGKSKLMVEQIL
                     TDLQKAQPDWSIALLRYFNPVGAHPSGDMGEDPQGIPNNLMPYIAQVAVGRRDSLAIF
                     GNDYPTEDGTGVRDYIHVMDLADGHVVAMEKLANKPGVHIYNLGAGVGNSVLDVVNAF
                     SKACGKPVNYHFAPRREGDLPAYWADASKADRELNWRVTRTLDEMAQDTWHWQSRHPQ
                     GYPD"
     gene            23201..24247
                     /gene="galT"
                     /locus_tag="ACHOSC_RS02265"
                     /old_locus_tag="pgaptmp_000453"
     CDS             23201..24247
                     /gene="galT"
                     /locus_tag="ACHOSC_RS02265"
                     /old_locus_tag="pgaptmp_000453"
                     /EC_number="2.7.7.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008108 - UDP-glucose:hexose-1-phosphate
                     uridylyltransferase activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006012 - galactose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="galactose-1-phosphate uridylyltransferase"
                     /protein_id="HGU4546465.1"
                     /translation="MTQFNPVDHPHRRYNPLTGQWILVSPHRAKRPWQGAQETPAKQV
                     LPAHDPDCFLCAGNVRVTGDKNPDYTRTYVFTNDFAALMSDTPDAPESNDPLMRCQSA
                     RGTSRVICFSPDHSKTLPELSVAALTEIVKTWQEQTAELGKTYPWVQVFENKGAAMGC
                     SNPHPHGQIWANSFLPNEAEREDRLQKEYFAEQKSPMLVDYVQRELADGSRTVVETEH
                     WLAVVPYWAAWPFETLLLPKAHVLRITDLTDAQRSDLALALKKLTSRYDNLFQCSFPY
                     SMGWHGAPFNGEENQHWQLHAHFYPPLLRSATVRKFMVGYEMLAETQRDLTAEQAAER
                     LRAVSDIHFRESGV"
     gene            24251..25399
                     /gene="galK"
                     /locus_tag="ACHOSC_RS02270"
                     /old_locus_tag="pgaptmp_000454"
     CDS             24251..25399
                     /gene="galK"
                     /locus_tag="ACHOSC_RS02270"
                     /old_locus_tag="pgaptmp_000454"
                     /EC_number="2.7.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001713229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004335 - galactokinase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006012 - galactose metabolic process
                     [Evidence IEA];
                     GO_process: GO:0046835 - carbohydrate phosphorylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="galactokinase"
                     /protein_id="HGU4546466.1"
                     /translation="MSLKEKTQSLFANAFGYPATHTIQAPGRVNLIGEHTDYNDGFVL
                     PCAIDYQTVISCAPRDDRKVRVMAADYENQLDEFSLNAPIVAHENYQWANYVRGVVKH
                     LQLRNNSFGGVDMVISGNVPQGAGLSSSASLEVAVGTVLQQLYYLPLDGAQIALNGQE
                     AENQFVGCNCGIMDQLISALGKKDHALLIDCRSLGTKAVSMPKGVAVVIINSNFKRTL
                     VGSEYNTRREQCETGARFFQQPALRDVTIEEFNAVAHELDPIVAKRVRHILTENARTV
                     EAASALEQGDLKRMGELMAESHASMRDDFEITVPQIDTLVEIVKAVIGDKGGVRMTGG
                     GFGGCIVALIPEELVPAVQQAVAEQYEAKTGIKETFYVCKPSQGAGQC"
     gene            25393..26433
                     /gene="galM"
                     /locus_tag="ACHOSC_RS02275"
                     /old_locus_tag="pgaptmp_000455"
     CDS             25393..26433
                     /gene="galM"
                     /locus_tag="ACHOSC_RS02275"
                     /old_locus_tag="pgaptmp_000455"
                     /EC_number="5.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004034 - aldose 1-epimerase activity
                     [Evidence IEA];
                     GO_process: GO:0006012 - galactose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="galactose-1-epimerase"
                     /protein_id="HGU4546467.1"
                     /translation="MLNETPALAPDGQPYRLLTLRNNAGMVVTLMDWGATLLSARIPL
                     SDGSVREALLGCASPECYQDQAAFLGASIGRYANRIANSRYTFDGETVTLSPSQGVNQ
                     LHGGPEGFDKRRWQIVNQNDRQVLFALSSDDGDQGFPGNLGATVQYRLTDDNRISITY
                     RATVDKPCPVNMTNHVYFNLDGEQSDVRNHKLQILADEYLPVDEGGIPHDGLKSVAGT
                     SFDFRSAKIIASEFLADDDQRKVKGYDHAFLLQAKGDGKKVAAHVWSADEKLQLKVYT
                     TAPALQFYSGNFLGGTPSRGTEPYADWQGLALESEFLPDSPNHPEWPQPDCFLRPGEE
                     YSSLTEYQFIAE"
     gene            26636..27388
                     /gene="gpmA"
                     /locus_tag="ACHOSC_RS02280"
                     /old_locus_tag="pgaptmp_000456"
     CDS             26636..27388
                     /gene="gpmA"
                     /locus_tag="ACHOSC_RS02280"
                     /old_locus_tag="pgaptmp_000456"
                     /EC_number="5.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000301556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004619 - phosphoglycerate mutase activity
                     [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-diphosphoglycerate-dependent
                     phosphoglycerate mutase"
                     /protein_id="HGU4546468.1"
                     /translation="MAVTKLVLVRHGESQWNKENRFTGWYDVDLSEKGVSEAKAAGKL
                     LKEEGYSFDFAYTSVLKRAIHTLWNVLDELDQAWLPVEKSWKLNERHYGALQGLNKAE
                     TAEKYGDEQVKQWRRGFAVTPPELTKDDERYPGHDPRYAKLSEKELPLTESLALTIDR
                     VIPYWNETILPRMKSGERVIIAAHGNSLRALVKYLDNMSEEEILELNIPTGVPLVYEF
                     DENFKPLKRYYLGNADEIAAKAAAVANQGKAK"
     gene            complement(27547..28599)
                     /gene="aroG"
                     /locus_tag="ACHOSC_RS02285"
                     /old_locus_tag="pgaptmp_000457"
     CDS             complement(27547..28599)
                     /gene="aroG"
                     /locus_tag="ACHOSC_RS02285"
                     /old_locus_tag="pgaptmp_000457"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001109196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase AroG"
                     /protein_id="HGU4546469.1"
                     /translation="MNYQNDDLRIKEIKELLPPVALLEKFPATENAANTVAHARKAIH
                     KILKGNDDRLLVVIGPCSIHDPVAAKEYATRLLALREELKDELEIVMRVYFEKPRTTV
                     GWKGLINDPHMDNSFQINDGLRIARKLLLDINDSGLPAAGEFLDMITPQYLADLMSWG
                     AIGARTTESQVHRELASGLSCPVGFKNGTDGTIKVAIDAINAAGAPHCFLSVTKWGHS
                     AIVNTSGNGDCHIILRGGKEPNYSAKHVAEVKEGLNKAGLPAQVMIDFSHANSSKQFK
                     KQMDVCADVCQQIAGGETAIIGVMVESHLVEGNQSLESGEPQAYGKSITDACIGWEDT
                     DALLRQLANAVKARRG"
     gene            28915..29295
                     /gene="ybgS"
                     /locus_tag="ACHOSC_RS02290"
                     /old_locus_tag="pgaptmp_000458"
     CDS             28915..29295
                     /gene="ybgS"
                     /locus_tag="ACHOSC_RS02290"
                     /old_locus_tag="pgaptmp_000458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbgS-like family protein"
                     /protein_id="HGU4546470.1"
                     /translation="MKMTKLATLFLTATLSLASGAALAADSGAQTNNGQANAAADAGQ
                     VAPDARENVAPNNVDNNGVNTGSGGTMLHSDGSSMNNDGMTKDEEHKNTMCKDGRCPD
                     INKKVQTGDGINNDVDTKTDGTTQ"
     gene            29409..30350
                     /gene="zitB"
                     /locus_tag="ACHOSC_RS02295"
                     /old_locus_tag="pgaptmp_000459"
     CDS             29409..30350
                     /gene="zitB"
                     /locus_tag="ACHOSC_RS02295"
                     /old_locus_tag="pgaptmp_000459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005385 - zinc ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006829 - zinc ion transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDF family zinc transporter ZitB"
                     /protein_id="HGU4546471.1"
                     /translation="MAHSHSHTSSHLPEDNNARRLLYAFGVTAGFMLVEVVGGFLSGS
                     LALLADAGHMLTDTAALLFALLAVQFSRRPPTIRHTFGWLRLTTLAAFVNAIALVVIT
                     ILIVWEAIERFRTPRPVEGGMMMAIAVAGLLANILSFWLLHHGSEEKNLNVRAAALHV
                     LGDLLGSVGAIIAALIIIWTGWTPADPILSILVSLLVLRSAWRLLKDSVNELLEGAPV
                     SLDIAELKRRMCREIPEVRNVHHVHVWMVGEKPVMTLHVQVIPPHDHDALLDQIQHYL
                     MDHYQIEHATIQMEYQPCHGPDCHLNEGVSGHSHHHH"
     gene            complement(30347..31066)
                     /gene="pnuC"
                     /locus_tag="ACHOSC_RS02300"
                     /old_locus_tag="pgaptmp_000460"
     CDS             complement(30347..31066)
                     /gene="pnuC"
                     /locus_tag="ACHOSC_RS02300"
                     /old_locus_tag="pgaptmp_000460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinamide riboside transporter PnuC"
                     /protein_id="HGU4546472.1"
                     /translation="MDFFSVQNILVHIPIGAGGYDLSWIEAVGTIAGLLCIGLASLEK
                     ISNYFFGLINVTLFGIIFFQIQLYASLLLQVFFFAANIYGWYAWSRQTSQNEAELKIR
                     WLPLPKALSWLAVCVVSIGLMTVFINPVFAFLTRLAVMIMQALGLQVVMPELQPDAFP
                     FWDSCMMVLSIVAMILMTRKYVENWLLWVIINVISVVIFALQGVYAMSLEYIILTFIA
                     LNGSRMWINSARERGSRALSH"
     gene            complement(31104..32147)
                     /gene="nadA"
                     /locus_tag="ACHOSC_RS02305"
                     /old_locus_tag="pgaptmp_000461"
     CDS             complement(31104..32147)
                     /gene="nadA"
                     /locus_tag="ACHOSC_RS02305"
                     /old_locus_tag="pgaptmp_000461"
                     /EC_number="2.5.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008987 - quinolinate synthetase A
                     activity [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="quinolinate synthase NadA"
                     /protein_id="HGU4546473.1"
                     /translation="MSVMFDPDTAIYPFPPKPTPLSIDEKAYYREKIKRLLKERNAVM
                     VAHYYTDPEIQQLAEETGGCISDSLEMARFGAKHPASTLLVAGVRFMGETAKILSPEK
                     TILMPTLQAECSLDLGCPVEEFNAFCDAHPDRTVVVYANTSAAVKARADWVVTSSIAV
                     ELIDHLDSLGEKIIWAPDKHLGRYVQKQTGGDILCWQGACIVHDEFKTQALTRLQEEY
                     PDAAILVHPESPQAIVDMADAVGSTSQLIAAAKTLPHQRLIVATDRGIFYKMQQAVPD
                     KELLEAPTAGEGATCRSCAHCPWMAMNGLQSIAEALEQEGSNHEVHVDERLRERALVP
                     LNRMLDFAATLRG"
     gene            complement(32396..32520)
                     /locus_tag="ACHOSC_RS02310"
                     /old_locus_tag="pgaptmp_000462"
     ncRNA           complement(32396..32520)
                     /ncRNA_class="other"
                     /locus_tag="ACHOSC_RS02310"
                     /old_locus_tag="pgaptmp_000462"
                     /product="RtT sRNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00391"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="rtT sRNA, processed from tyrT transcript; Derived
                     by automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF00391"
     gene            complement(32552..32627)
                     /locus_tag="ACHOSC_RS02315"
                     /old_locus_tag="pgaptmp_000463"
     tRNA            complement(32552..32627)
                     /locus_tag="ACHOSC_RS02315"
                     /old_locus_tag="pgaptmp_000463"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(32592..32594),aa:Lys,seq:ttt)
     gene            complement(32661..32736)
                     /locus_tag="ACHOSC_RS02320"
                     /old_locus_tag="pgaptmp_000464"
     tRNA            complement(32661..32736)
                     /locus_tag="ACHOSC_RS02320"
                     /old_locus_tag="pgaptmp_000464"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(32701..32703),aa:Lys,seq:ttt)
     gene            complement(32883..32958)
                     /locus_tag="ACHOSC_RS02325"
                     /old_locus_tag="pgaptmp_000465"
     tRNA            complement(32883..32958)
                     /locus_tag="ACHOSC_RS02325"
                     /old_locus_tag="pgaptmp_000465"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(32923..32925),aa:Lys,seq:ttt)
     gene            complement(32962..33037)
                     /locus_tag="ACHOSC_RS02330"
                     /old_locus_tag="pgaptmp_000466"
     tRNA            complement(32962..33037)
                     /locus_tag="ACHOSC_RS02330"
                     /old_locus_tag="pgaptmp_000466"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(33002..33004),aa:Val,seq:tac)
     gene            complement(33187..33262)
                     /locus_tag="ACHOSC_RS02335"
                     /old_locus_tag="pgaptmp_000467"
     tRNA            complement(33187..33262)
                     /locus_tag="ACHOSC_RS02335"
                     /old_locus_tag="pgaptmp_000467"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(33227..33229),aa:Lys,seq:ttt)
     gene            complement(33265..33340)
                     /locus_tag="ACHOSC_RS02340"
                     /old_locus_tag="pgaptmp_000468"
     tRNA            complement(33265..33340)
                     /locus_tag="ACHOSC_RS02340"
                     /old_locus_tag="pgaptmp_000468"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(33305..33307),aa:Val,seq:tac)
     gene            complement(33476..33551)
                     /locus_tag="ACHOSC_RS02345"
                     /old_locus_tag="pgaptmp_000469"
     tRNA            complement(33476..33551)
                     /locus_tag="ACHOSC_RS02345"
                     /old_locus_tag="pgaptmp_000469"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(33516..33518),aa:Lys,seq:ttt)
     gene            complement(33716..34507)
                     /gene="cpoB"
                     /locus_tag="ACHOSC_RS02350"
                     /old_locus_tag="pgaptmp_000470"
     CDS             complement(33716..34507)
                     /gene="cpoB"
                     /locus_tag="ACHOSC_RS02350"
                     /old_locus_tag="pgaptmp_000470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA];
                     GO_process: GO:0070206 - protein trimerization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein CpoB"
                     /protein_id="HGU4546474.1"
                     /translation="MSSNFRHQLLSLSLLVGIAAPWAAFAQAPISSVGSGSVEDRVTQ
                     LERISNAHSQLLTQLQQQLSDNQSDIDSLRGQIQENQYQLNQVVERQKQILLQIDSLS
                     SGGAAAQSTSSDQSGAAASTTPTADAGTANAGAPVKSGDANTDYNAAIALVQDKSRQD
                     DAMVAFQNFIKNYPDSTYLPNANYWLGQLNYNKGKKDDAAYYFASVVKNYPKSPKAAD
                     AMFKVGVIMQDKGDTAKAKAVYQQVISKYPGTDGAKQAQKRLNAM"
     gene            complement(34517..35038)
                     /gene="pal"
                     /locus_tag="ACHOSC_RS02355"
                     /old_locus_tag="pgaptmp_000471"
     CDS             complement(34517..35038)
                     /gene="pal"
                     /locus_tag="ACHOSC_RS02355"
                     /old_locus_tag="pgaptmp_000471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan-associated lipoprotein Pal"
                     /protein_id="HGU4546475.1"
                     /translation="MQLNKVLKGLMIALPVMAIAACSSNKNASNDGSEGMLGAGTGMD
                     ANGGNGNMSSEEQARLQMQQLQQNNIVYFDLDKYDIRSDFAQMLDAHANFLRSNPSYK
                     VTVEGHADERGTPEYNISLGERRANAVKMYLQGKGVSADQISIVSYGKEKPAVLGHDE
                     AAYSKNRRAVLVY"
     gene            complement(35073..36365)
                     /gene="tolB"
                     /locus_tag="ACHOSC_RS02360"
                     /old_locus_tag="pgaptmp_000472"
     CDS             complement(35073..36365)
                     /gene="tolB"
                     /locus_tag="ACHOSC_RS02360"
                     /old_locus_tag="pgaptmp_000472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001646851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0017038 - protein import [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Tol-Pal system beta propeller repeat protein
                     TolB"
                     /protein_id="HGU4546476.1"
                     /translation="MKQALRVAFGFLILWASVLHAEVRIVIDSGVDSGRPIGVVPFQW
                     AGPGAAPEDIGGIVAADLRNSGKFNPLDRARLPQQPGSAQEVQPAAWSALGIDAVVVG
                     QVTPNPDGSYNVAYQLVDTGGAPGTVLAQNSYKVNKQWLRYAGHTASDEVFEKLTGIK
                     GAFRTRIAYVVQTNGGQFPYELRVSDYDGYNQFVVHRSPQPLMSPAWSPDGSKLAYVT
                     FESGRSALVIQTLANGAVRQVASFPRHNGAPAFSPDGSKLAFALSKTGSLNLYVMDLA
                     SGQIRQVTDGRSNNTEPTWFPDSQNLAFTSDQAGRPQVYKVNINGGAPQRITWEGSQN
                     QDADVSSDGKFMVMVSSNGGQQHIAKQDLATGGVQVLSSTFLDETPSLAPNGTMVIYS
                     SSQGMGSVLNLVSTDGRFKARLPATDGQVKFPAWSPYL"
     gene            complement(36498..37763)
                     /gene="tolA"
                     /locus_tag="ACHOSC_RS02365"
                     /old_locus_tag="pgaptmp_000473"
     CDS             complement(36498..37763)
                     /gene="tolA"
                     /locus_tag="ACHOSC_RS02365"
                     /old_locus_tag="pgaptmp_000473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0019534 - toxin transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0043213 - bacteriocin transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell envelope integrity protein TolA"
                     /protein_id="HGU4546477.1"
                     /translation="MSKATEQNDKLKRAIIISAVLHVILFAALIWSSFDENIEASAGG
                     GGGSSIDAVMVDSGAVVEQYKRMQSQESSAKRSDEQRKMKEQQAAEELREKQAAEQER
                     LKQLEKERLAAQEQKKQAEEAAKQAELKQKQAEEAAAKAAADAKAKAEADAKAAEEAA
                     KKAAADAKKKAEAEAAKAAAEAQKKAEAAAAALKKKAEAAEAAAAEARKKAATEAAEK
                     AKAEAEKKAAAEKAAADKKAAAEKAAADKKAAEKAAAEKAAADKKAAAEKAAADKKAA
                     AAKAAAEKAAAAKAAAEADDIFGELSSGKNAPKTGGGAKGNNASPAGSGNTKNNGASG
                     ADINNYAGQIKSAIESKFYDASSYAGKTCTLRIKLAPDGMLLDIKPEGGDPALCQAAL
                     AAAKLAKIPKPPSQAVYEVFKNAPLDFKP"
     gene            complement(37828..38256)
                     /gene="tolR"
                     /locus_tag="ACHOSC_RS02370"
                     /old_locus_tag="pgaptmp_000474"
     CDS             complement(37828..38256)
                     /gene="tolR"
                     /locus_tag="ACHOSC_RS02370"
                     /old_locus_tag="pgaptmp_000474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015965300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin uptake protein TolR"
                     /protein_id="HGU4546478.1"
                     /translation="MARARGRGRRELKSEINIVPLLDVLLVLLLIFMATAPIITQSVE
                     VDLPDATESQAVSSNDNPPVIVEVSGIGQYTVVVEKDRLERLPPEQVVAEVSSRFKAN
                     PKTVFLIGGAKDVPYDEIIKALNLLHSAGVKSVGLMTQPI"
     gene            complement(38260..38952)
                     /gene="tolQ"
                     /locus_tag="ACHOSC_RS02375"
                     /old_locus_tag="pgaptmp_000475"
     CDS             complement(38260..38952)
                     /gene="tolQ"
                     /locus_tag="ACHOSC_RS02375"
                     /old_locus_tag="pgaptmp_000475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Tol-Pal system protein TolQ"
                     /protein_id="HGU4546479.1"
                     /translation="MTDMNILDLFLKASLLVKLIMLILIGFSIASWAIIIQRTRILNA
                     AAREAEAFEDKFWSGIELSRLYQESQGKRDNLTGSEQIFYSGFKEFVRLHRANSHAPE
                     AVVEGASRAMRISMNRELENLETHIPFLGTVGSISPYIGLFGTVWGIMHAFIALGAVK
                     QATLQMVAPGIAEALIATAIGLFAAIPAVMAYNRLNQRVNKLELNYDNFMEEFTAILH
                     RQAFTVSESNKG"
     gene            complement(38949..39353)
                     /gene="ybgC"
                     /locus_tag="ACHOSC_RS02380"
                     /old_locus_tag="pgaptmp_000476"
     CDS             complement(38949..39353)
                     /gene="ybgC"
                     /locus_tag="ACHOSC_RS02380"
                     /old_locus_tag="pgaptmp_000476"
                     /EC_number="3.1.2.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tol-pal system-associated acyl-CoA thioesterase"
                     /protein_id="HGU4546480.1"
                     /translation="MNTTLFRWPVRVYYEDTDAGGVVYHASYVAFYERARTEMLRHHH
                     FSQQALMAERVAFVVRKMTVEYYAPARLDDMLEIQTEITSMRGTSLVFTQRIVNAENT
                     LLNEAEVLVVCVDPLKMKPRALPKSIVAEFKQ"
     gene            complement(39503..39796)
                     /gene="ybgE"
                     /locus_tag="ACHOSC_RS02385"
                     /old_locus_tag="pgaptmp_000477"
     CDS             complement(39503..39796)
                     /gene="ybgE"
                     /locus_tag="ACHOSC_RS02385"
                     /old_locus_tag="pgaptmp_000477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyd operon protein YbgE"
                     /protein_id="HGU4546481.1"
                     /translation="MSKIIATLYAVMDKRPLRALSFVMALLLAGCMFWDPSRFAAKTS
                     ELEIWHGLLLMWAVCAGVIHGVGFRPQKVLWQGIFCPLLADIVLIVGLIFFFF"
     gene            complement(39796..39909)
                     /gene="cydX"
                     /locus_tag="ACHOSC_RS02390"
                     /old_locus_tag="pgaptmp_000478"
     CDS             complement(39796..39909)
                     /gene="cydX"
                     /locus_tag="ACHOSC_RS02390"
                     /old_locus_tag="pgaptmp_000478"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016682 - oxidoreductase activity, acting
                     on diphenols and related substances as donors, oxygen as
                     acceptor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome bd-I oxidase subunit CydX"
                     /protein_id="HGU4546482.1"
                     /translation="MWYFAWILGTLLACSFGVITALALEHVESGKAGQEDI"
     gene            complement(39924..41063)
                     /gene="cydB"
                     /locus_tag="ACHOSC_RS02395"
                     /old_locus_tag="pgaptmp_000479"
     CDS             complement(39924..41063)
                     /gene="cydB"
                     /locus_tag="ACHOSC_RS02395"
                     /old_locus_tag="pgaptmp_000479"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000568269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome d ubiquinol oxidase subunit II"
                     /protein_id="HGU4546483.1"
                     /translation="MIDYEVLRFIWWLLVGVLLIGFAVTDGFDMGVGMLTRFLGRNDT
                     ERRIMINSIAPHWDGNQVWLITAGGALFAAWPMVYAAAFSGFYVAMILVLASLFFRPV
                     GFDYRSKIEETRWRNMWDWGIFIGSFVPPLVIGVAFGNLLQGVPFNVDEYLRLYYTGN
                     FFQLLNPFGLLAGVVSVGMIITQGATYLQMRTVGELHLRTRATAQVAALVTLVCFALA
                     GVWVMYGIDGYVVKSTMDHYAASNPLNKEVVREAGAWLVNFNNTPILWAIPALGVVLP
                     LLTILTARMDKAAWAFVFSSLTLACIILTAGIAMFPFVMPSSTMMNASLTMWDATSSQ
                     LTLNVMTWVAVVLVPIILLYTAWCYWKMFGRITKEDIERNTHSLY"
     gene            complement(41079..42647)
                     /gene="cydA"
                     /locus_tag="ACHOSC_RS02400"
                     /old_locus_tag="pgaptmp_000480"
     CDS             complement(41079..42647)
                     /gene="cydA"
                     /locus_tag="ACHOSC_RS02400"
                     /old_locus_tag="pgaptmp_000480"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415261.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0070069 - cytochrome complex [Evidence
                     IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_process: GO:0019646 - aerobic electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome ubiquinol oxidase subunit I"
                     /protein_id="HGU4546484.1"
                     /translation="MLDIVELSRLQFALTAMYHFLFVPLTLGMAFLLAIMETVYVLSG
                     KQIYKDMTKFWGKLFGINFALGVATGLTMEFQFGTNWSYYSHYVGDIFGAPLAIEGLM
                     AFFLESTFVGLFFFGWDRLGKVQHMCVTWLVALGSNLSALWILVANGWMQNPIASDFN
                     FETMRMEMVSFSELVLNPVAQVKFVHTVASGYVTGAMFILGISAWYMLKGRDFAFAKR
                     SFAIAASFGMAAVLSVIVLGDESGYEMGDVQKTKLAAIEAEWETQPAPAAFTLFGIPD
                     QEEETNKFAIQIPYALGIIATRSVDTPVIGLKELMVQHEERIRNGMKAYSLLEQLRSG
                     STDQAVRDQFNSMKKDLGYGLLLKRYTPNVADATEAQIQQATKDSIPRVAPLYFAFRI
                     MVACGFLLLAIIALSFWSVIRNRIGEKKWLLRAALYGIPLPWIAVEAGWFVAEYGRQP
                     WAIGEVLPTAVANSSLTAGDLIFSMVLICGLYTLFLVAELFLMFKFARLGPSSLKTGR
                     YHFEQSSTTTQPAR"
BASE COUNT        10702 a        11457 c        10495 g        10305 t
ORIGIN      
        1 tccccgacca gaagcgtaaa tcccagcacc gttaaacaca gaactatgac tgccgcaagg
       61 gcatatttcg tcagcatgtt tatattgcct ccatgcgaag aagggactac catccaaatt
      121 gttcaggttt gggtaggtag ccccagggtt gatagaaata tcgtcctggg acttcatctt
      181 tccagacctc agagtaaacc tgaaaccgga aagcttcagg cacccgcagt tatcttaccc
      241 ttgcttagca aaatagctgt ttatttatac agtaagtcct gtttcggact tctcactccc
      301 catttgatca actgaagctg aacccgtttt tttctgcatg cctcaatcgt caaaattctt
      361 cgccaccagc cagccgtgcc ataacttgtc acattgcaaa agtagccaaa ttgcttcagt
      421 caccatcacc aaaaaaagaa acattccaga acttccgcat ctcgcccagc cgctccgact
      481 tctatactga atagaaaacg ccaacttaag agaaacctat gccattaccc gattttcatg
      541 tttctgaacc ttttaccctc ggtattgaac tggaaatgca ggtggttaat ccgccgggct
      601 atgacttaag ccaggactct tcaatgctga ttgacgcggt taaaaatgag atcacggccg
      661 gagaggtaaa gcacgatatc accgaaagta tgctggagct ggcgacggat gtttgccgtg
      721 atatcaacca ggctgccggg cagttttcag cgatgcagaa agtcgtattg caggcagccg
      781 cagaccatca tctggaaatt tgcggcggtg gcacgcaccc gtttcagaaa tggcagcgtc
      841 aggaggtatg cgacaacgaa cgctatcaac gaacgctgga aaactttggc tatctcatcc
      901 agcaggcgac cgtttttggt cagcatgtcc atgttggctg cgccagtggc gatgacgcca
      961 tttatttgct gcacggcctg tcacggtttg tgccgcactt catcgccctt tccgccgcgt
     1021 cgccgtatat gcagggaacg gatacgcgtt ttgcctcctc acgaccgaat attttttccg
     1081 cctttcccga taatggcccg atgccgtggg tcagtaactg gcaacaattt gaagccctgt
     1141 ttcgctgcct gagttacacc acgatgatcg acagcattaa agatctgcac tgggatattc
     1201 gccccagtcc tcattttggc acggtggagg ttcgggtgat ggataccccg ttaaccctta
     1261 gccacgcggt aaatatggcg ggattaattc aggccaccgc ccactggtta ctgacggaac
     1321 gcccgtttaa acatcaggag aaagattacc tgctgtataa attcaaccgt ttccaggcct
     1381 gccgctatgg gctggaaggc gtcattaccg atccgcacac tggcgatcgt cgaccgctaa
     1441 cggaagatac cttgcgattg ctggaaaaaa tcgccccttc cgcacataaa atgggtgcat
     1501 cgagcgcgat tgaggccctg catcgccagg tcgtcagcgg tctgaatgaa gcgcagctga
     1561 tgcgcgattt cgtcgccgat ggtggctcgc tgattgggct ggtgaaaaag cattgtgaga
     1621 tctgggccgg tgactaagta gggaattgcc ttacagaaga atatccctga caatggtgtt
     1681 ttaacttaca tttaacaatg ttatgaaaca ccctttagaa accttgacta ccgcagcagg
     1741 cattttgctg atggctttcc tctcttgcct gctgctgccc gcccccgcac tggggctgac
     1801 gctggcacaa aaactggtga ccatgttcca tctgatggat cttagccagc tttacacttt
     1861 attgttttgt ctgtggtttt tagtgctggg cgctattgag tattttgttc tgcgctttat
     1921 ctggcaacgc tggttctcgc tggcggatta aacgtcgatt atcgccgtac ttcgcggcat
     1981 actttgctta ttctccttcg cctaaaggaa cgtttatgga taagcaatca ctgcacgaaa
     2041 cggcgaaacg cctagccctt gagttaccct ttgtcgagct ttgctggcct tttggcccgg
     2101 agttcgatgt ttttaaaatt ggcggcaaaa tttttatgct gtcgtcggag ctgcgcggcg
     2161 tcccctttat caatctgaag tccgatccac aaaaatccct gttaaatcag cagatatacc
     2221 cgagcattaa gccagggtat cacatgaata aaaagcactg gatttcggtg tatcccggcg
     2281 aggaaatctc cgaagcgtta cttcgcgatc tgatcaacga ttcgtggaat ctggtggttg
     2341 atggtctggc taaacgcgat caaaaaagag tgcgtccagg ctaaagcgga aatctatagc
     2401 gcatttttct cgcttaccat ttctcgttga accttgtaat ctgctggcac gcaaaattac
     2461 tttcacatgg agtctttatg gatatcattt ctgtcgcctt aaagcgtcat tccactaagg
     2521 catttgatgc cagtaaaaaa cttaccccgg aacaggccga gcagatcaaa actctcctgc
     2581 aatacagccc atccagcacc aactcccagc cgtggcattt tattgttgcc agcacggaag
     2641 aaggtaaagc gcgtgttgcc aaatccgctg ccggtaatta tgtgttcaac gaacgtaaaa
     2701 tacttgatgc ctcgcacgtc gtggtgttct gtgcaaaaac cgcgatggac gatgcctggc
     2761 tgaagctggt tgttgaccag gaagatgctg atggccgctt tgccacgccg gaagcgaaag
     2821 ccgcgaacga taaaggtcgc aagttcttcg ctgatatgca ccgtaaagat ctgcatgatg
     2881 atgcagagtg gatggcaaaa caggtttatc tcaacgtcgg taacttcctg ctcggcgtgg
     2941 cggcgctggg tctggacgcg gtacccatcg aaggttttga cgccgccatc ctcgatgcag
     3001 aatttggtct gaaagagaaa ggctacacca gtctggtggt tgttccggta ggtcatcaca
     3061 gcgttgaaga ttttaacgct acgctgccga aatctcgtct gccgcaaaac atcaccttaa
     3121 ccgaagtgta attctctctt gccgggcatc tgcccggcta tttcccctca gattctcctg
     3181 atttgcataa ccctgtttca gccgtcatca taggctgctg ttgtataaag gagacgttat
     3241 gcaggattta atatcccagg ttgaagattt agcgggtatt gagatcgatc acaccacctc
     3301 gatggtgatg attttcggta ttatttttct gaccgccgtc gtggtgcata ttattttgca
     3361 ttgggtggta ctgcggacct tcgaaaaacg tgccatcgcc agttcacggc tttggttgca
     3421 aatcattacc cagaataaac tcttccaccg tttagctttt accctgcagg ggattatcgt
     3481 caatattcag gcggtattct ggctgcaaaa aggcaccgaa gcggcagata ttctgactac
     3541 ctgcgcgcag ttgtggatca tgatgtatgc gctgctttca gtcttctcgt tgctggatgt
     3601 tattttgaat ctggcgcaga aattcccggc ggcatctcag ttaccgctga aagggatatt
     3661 tcaggggatt aaactgatcg gcgcaatcct ggtcggcatt ttgatgatct cgctgctgat
     3721 tggtcagtcg ccagcgattc tgatcagcgg tcttggtgca atggctgccg tgctgatgtt
     3781 ggtttttaaa gatccgattc ttggtctggt ggcaggtatt cagctttccg cgaacgatat
     3841 gctgaaactg ggcgactggc tggagatgcc gaaatacggc gcggatggcg cggtgatcga
     3901 tattgggtta accaccgtca aagtgcgtaa ctgggacaat accattacca ctattcccac
     3961 ctggtctctg gtttctgact cctttaaaaa ctggagcggg atgtcagcat ctggcgggcg
     4021 acgtattaag cgcagtatca gtattgatgt caccagtatt cgttttcttg atgaagacga
     4081 aatgcaacgt ctgaataaag cgcacttgtt aaaaccttat ttaaccagcc gccatcagga
     4141 aattaatgag tggaatcgcc agcaaggttc tacggagtcg atattaaacc tgcgccgaat
     4201 gaccaatatt ggaacctttc gtgcctatct gaacgaatat ctgcgtaacc atccgcggat
     4261 tcgtaaagat atgaccttaa tggtacgcca actggctcca ggtgataacg gtttaccgct
     4321 cgagatctac gcgtttacta acaccgtggt gtggctggaa tatgaaagca ttcaggctga
     4381 tatattcgat cacatatttg cgattgtcga agagtttggt ctgcgacttc atcagtcgcc
     4441 aaccggcaat gatattcgct ctctggcggg tgcatttaag cagtaattaa aaaaaccgct
     4501 ctcatcgaat ggatgagagc ggacatcgtt ttgtcggatg tagcgtgaat gccttatttc
     4561 cgacgcagcg ttttaaatgc cacaaacagg aatacaatcc acaccggcag caggatcgct
     4621 gacaagcgca tatcatccat cgtgcacatc agcagcaaaa tcatgccgag gaaggcaatg
     4681 caaagatagt tgccgaacgg atagagcagc gccttaaact gtgtttcacg cccctgacgt
     4741 cgcatcgctg cacgaaaacg cagatgcgcc agacagatca taatccagtt caacagcagc
     4801 gttgctacca ccagcgccat cagcagacca aacgcttttt gcggcagcag atagttgatt
     4861 aataccacca gcgaagtgat cgctccggaa agcatcagcg agttaatcgg cacaccgcga
     4921 cggctgacgc gagtcaaaaa ctttggcgca ttaccctgca cagaaaggcc aaacagcatg
     4981 cggctgttag agtaaacccc gctgttatac actgacagcg atgctaccag aatgacgaag
     5041 ttcagcgcag aagctaccac gttgctgtcg agattatgga aaatcatcac aaacgggcta
     5101 ctgttggatt tcacttccac ccacggatag agcgccagta aaaccaccag tgaaccgatg
     5161 taaaacagca ggatgcgata caccacctga tttaccgctt ttggaatgct tttttccgga
     5221 tcgcgcgctt cagcggcagt aatcccaatc agctccagac cgccgaagga gaacataatt
     5281 accgccagcg acaaaatcag cccattccag ccggtggcaa agaaaccacc gtagcgccag
     5341 aggttgtcga tactggcttt ctcgccgccg tgaccagaaa acagcagcca caggccaaag
     5401 ccgatcatac cgatgatcgc cagcacttta atcagcgcaa accagaactc ggtttcacca
     5461 tataagcgca cgttcaccag gttaacggcg ttgatgataa taaagaaggc ggcagcccaa
     5521 atccacgttg gaacatccgg gaaccagtac tgcatataga tgcccgcagc ggtcagctct
     5581 gccattccca ccagcacgaa cattacccag tagttccagc cagagaggaa gcccgcaaac
     5641 ggtccccagt atttataggc aaagtgggca aatgaaccgg ataccggctc ttcgaccacc
     5701 atctcgccaa gctggcgcat aatcaggaaa gcgatgatcc cggcgacgcc gtagcccagc
     5761 aatacagccg gacccgccat ctgaatcgcc gggccaatgc caagaaacag accagtacca
     5821 attgcgccac ccagcgcaat cagttgaata tgacggttat gtaatccgcg atgaagcgtc
     5881 ggctcttgat tcgacgcagt atcttccgat acggttgacg cgtttttcac gcctttcccc
     5941 tgtgtgtctt ttttgttgag gggcaccttt taacatttag tgcccatcgt cgcaagacac
     6001 aatccacacg gttaaacggg gtatcctgct tttatttccg tacccgatgt cggtgcagcc
     6061 acatcagctt atacgccgcc gggataataa acagcgacag caaaggtgcg gtgatcatgc
     6121 cgccaatcat cggtgcggca atccggctca tcacctctga accagccccc gttccccaca
     6181 gaatcggcag cagaccggcg ataatcaccg ccaccgtcat cgctttcggg cgcacgcgca
     6241 ggaccgcgcc gtgatataac gcctcatcca gcttctgctc gctgaatgtt tgtggattat
     6301 tcaacgacgg ctcggcctct atggcgtgac gtaaatacat cagcatcacc acgccaaatt
     6361 cggcggcgac cccggcgagg gcgataaagc cagtgcccgt cgccacggaa agatgaaagc
     6421 ccatccacca cagcaaccag atgccgccca ccagcgcaaa cggtacgctg ctgataatca
     6481 gcaacgcttc gcccacccga cggaacgcca gatacaacag cacgaagata atcatcaacg
     6541 tcatcggcac catgagttta agcttatggt tggcgcgctc cagtagctcg aactgcccgg
     6601 agaatgccac gctggtgccc ggttttaact gcactttttc agctatcgct ttttgcaaat
     6661 cgtgaaccac cgacaccatg tcacgatcgc gggcatcgat ataaatccag ctcgtcgggc
     6721 gcgcattctc ggttttcagc attgacggtc cggtagagac tttaatgtcg gccacgtctg
     6781 ccagggtgat ttgctgcttc atcggcgtca ggatcggcaa ctggcgcagt gcctgcggac
     6841 tatcgcgcca gctttgcgga taacgcagat taattggata acgggcaatc ccttccaccg
     6901 tttcgccaac catcgccccg cccaccgcag aagtcacaaa caactgcaca tccgccaccg
     6961 tcataccgta acgtgcggct ttttcacggt taatctcaac gttgatatag cgcccacctt
     7021 ccagacgctc ggctagagca gaagccacgc ctggcaccgt tcgcgctact tcttcaattt
     7081 gctcagccat cgcgtcgata tccgccagca cagtgccgga aactttaatg ccgatggggc
     7141 ttttaatgcc ggttgagagc atatcgatac ggttacgaat tggcggcacc cacagattcg
     7201 ccagccccgg cagacgcacg gtgttatcca gttcctcaat gattttgtcc attgtcatgc
     7261 ctggccgcca ctgctcctgc ggcttaagct ggatggtcgt ttctaccatc tccagcggag
     7321 cagaatcggt ggcggtttcc gctttcccgg ttttgccaaa tacccgcgcc acttcaggta
     7381 cgctcataat tagcttgtcg gttttttgca gcatactcgc cgcctctgct gcggaaatcc
     7441 ccggcagcgt cgatggcata tacaacaggt cgccttcatt gatctgcggt aaaaattccc
     7501 cgccaacttt attgagcggc cagagaaccg tcagcaccga aagcgctgcc accaacagcg
     7561 tggttttcgg ccagtgcagt actttcagca acagcggatg ataaacacga atcaaaaagc
     7621 gattgagcgg gttactgctt tccggcggaa ttttgccacg gatccagtaa cccatcagga
     7681 tagggatcac tacgatcgcc agcagcgccg cacccgccat cgcatacgtt ttggtgaacg
     7741 ccagcgggcc aaacagacgc ccttcctgcc cttccagggt gaagatcggg ataaacgaca
     7801 acgtgataat cagcagactg ataaatagcg ccggacccac ttcaacagat gcatcggtga
     7861 tcacctgcca gcgcgtttta ttatccagcg tggcgtcagg atgctggtgc tgccactctt
     7921 ccagccgttt atgtgcattt tcgatcatga cgatagcggc atcgaccatc gccccgacgg
     7981 caatcgcaat gccgcccaac gacataatat tggcattcag tccctggaag tgcatgacaa
     8041 taaaagcaat acacaacccc agcggcaacg aaataatcgc caccagcgcc gagcgcacat
     8101 gccagagaaa cagcgcacag actaccgcca ccacaataaa ctcttccagc aacttgccgc
     8161 tgaggttgtc gatggcgcgg tcaatgagct ggctgcgatc gtatgttgta actatctcca
     8221 cgccttccgg cagactactt ttcagcgttt ccagtttgtc cttcacggcg gcgatcactt
     8281 ctcgggcgtt tttgccggat cgcaggatca ccaccccgcc cgccacttcg ccttcgccgt
     8341 ttagttcggc aatgccccgg cgcatctccg ggccaatctg gaccttcgca acatcgcgca
     8401 gataaacggg cacgccattt tcactggctt ttaaaacgat gtgattaaag tcgtcgagcg
     8461 tttgcagata gccgctggcg cgcaccatat attccgcttc cgccagttcg atcgacgaac
     8521 cgcccgcttc ctggtttgaa gcatccagcg cgctttttac ttcggcgaga ctgatgccat
     8581 actgcgccag gcgctgggga tcgataacca cctgatactc tttcaccaca ccgcccaccg
     8641 acgccacttc cgcaacgtca gggatggttt tcagctcata tttgagaaac cagtcctgca
     8701 atgagcgtaa atcggccaga tcgtgcttac cgctgcgatc caccagtgca tattcataga
     8761 tccagccaac acccgtggca tctggtccca gctcggcgct gactcccgca ggcagcttac
     8821 cctgtacctg gttgaggtac tccagcaccc gcgaacgcgc ccagtacgga tcggtgccat
     8881 cttcgaaaat gacatacaca taagagtcgc caaactgtga gaaaccgcgc acagtcttcg
     8941 cgccaggcac cgacaacatg gtggtggtta gcggataagt cacctgattt tcaacgattt
     9001 gtggtgcctg accgggatag ctggttttaa taataacctg cacatcggag agatccggca
     9061 gcgcatccac tggcgtatta atgatggtcc aggtgcccca gatgctcaga aacaacgcgc
     9121 ccatcagcac caaaaaacgg ttcgccaccg agcgacgaat aatccattca atcattggtt
     9181 attccctcaa tgcgcatggg tagcactttc agagcgcatc cgctccagtg cgccagaaat
     9241 attggcttcg gaatcaatca ggaacaggcc gctggaaacc accttttcac cttccgccag
     9301 accagagcgt aatgcggtga cgccttgcga tacctggaaa acagcaacgc gtttcggtac
     9361 aaagcgcccg tcggcatcaa cggtaatcac ccgctgttcg ctgccggtat caatcagcgc
     9421 ttgtgacgga atgagcagca tcggttcgct ggcggtgttg agttgcaacc aggcgttcat
     9481 gcccggtttt agcgcctcgt cggcgttgtc gacttccaga cgcagctgca gcgtgcgggt
     9541 cgcggcatcc acgccaggta gcagcgtcca tttgcggatg gtgagtgttt tatccggtcg
     9601 cgccggaacg gtgagggtaa actgcgaggc atctttcacc agccaggcga tggactccgg
     9661 gatcgcagca gtgacccaca ccgggtccat accctgaatt ttcgcgacca cgttatcttt
     9721 ggcgatattc attcccgcgc gcagatcaaa cgcggtgatc acgccatcaa tgggcgcttt
     9781 gagcgtaaag cgagtctgga ttttttgcgt ggcgatcagg cggcgaatat ccgcctccgg
     9841 cattcccgcc agtcgcagcc gctcaagaat gccttcagtc tgggtcgccg taccgccggt
     9901 ttcgcgcagc agtaaatact cactctgcgc ttccacccag tcaggaatgg tcaggtcgag
     9961 aagcggtgtg cccttttgta ctttatcgcc cacggtaagc ggatacacct tgtcgataaa
    10021 cccggcagcg cgagcctgca caatggcata ctgatactcg ttgtaactga cattcgccgg
    10081 gaaactctgg gcaaaagtca gcggcccgcg cgtgacggta gccgttttca cccccagatt
    10141 ctgcgtctga gtcgggtcaa tgcgcacacc agacgcagaa ctctcttcat cggcatattt
    10201 cggcaccaga tccatatcca taaacggcga tttacctggt ttatcgaacc gcgtattggg
    10261 gtacatcggg tcgtaccaga ataagatttt acgttctgcg gtcgacgttt tttctgcggg
    10321 tggttcagcc tttgcaaacc aggtaaaacc tgccgcagaa ataataccgc ccgcgatcat
    10381 gctgccgata ataagcgcga tttttttcat ctcattaaac ctgggttact ggctgacttt
    10441 aatatcctgt aataaagaaa ggttgccctg ctggacaaaa ttaaacgcca ctttgtcgcc
    10501 agttttaatt tcactcattt tcgtctgcgg agtgatggta aagcgcatgg tcatctccgg
    10561 ccagttcacg gcagcaatcg gatcgtgatg gatggtgatt tttttgcttt ccagatcaat
    10621 accctttacc acgccagtgg cgctaataac ctgcggttgt acttcgctca tggtttcatg
    10681 atgatgttcg ttagcctggg cattaaagcc aataacggta aacagactga acattgcgac
    10741 ttgcagtgct tttttcattt attctctcct ggagttaaaa gtcatactct aaataattcg
    10801 agttgcagga aggcgacaag cgagtgaatc cccaggagct tacataagta agtgactggg
    10861 gtgagcgaac gcagacgcag cacatgcaac ttgaagtatg acgagaatat ttacagcatg
    10921 ttccagccga tcagcatatt ccaccaccac acgccgaggg tgatatgcac caggaaggtt
    10981 aatatcgtca gtaccgcacc gaccaaccac caggatttaa tatcgttata acccacgcca
    11041 aagatgaccg gacctgccgc gccgccatag tgagtaacca tgccgccata ggagttggag
    11101 aacaacagtg ccagcgcggt taacattaac ggtgcgccgg agacgttcgc cagcatggca
    11161 aataccggta acatagcaac gatataggca ctaccggaag cgaagaaata acgcacgata
    11221 atgctgagga aaataataac gaagaaagca acgttaccgt gaccatcaaa tgccaggtta
    11281 tttttaaaga cttcagctaa ccattcgaag aatttaactt tcgataataa ggagcttaat
    11341 ccgataatac cgccgtacca gattaaggta ttccagccgc ctttattttt aaccacgtct
    11401 tcccaggtaa cgatacccag cagcagcatg gtcgccataa caacgattgc cacagtggat
    11461 tcatcaaccc ccagagactt actgaaaatc cagcccagta gcgccagcac aaagacaccg
    11521 agcagcattt tttcgcggat tttcatcggt cccagttcgg caaggcccgc tttagcgatg
    11581 gttttattat ccaccttctt aatttctggc ggatacatgg tataaatcac cagtggggtg
    11641 accagcagca taatgatgcc cggcaatccg gcggccagtg cccatccacc ccagctaatt
    11701 tgcaggtgca gaatgtcgtt gatcattttc agcgccagaa tgttccccgc cattgcggta
    11761 aagaacatat agctggtggt tttggtgacc atgtaaatgg acatcatcag gtaatgtccg
    11821 acacgacgcg gacttttttc cggttcggac cccaaagcca ccgccacgct gttgatgatc
    11881 ggtaacacaa taccgcccgc acgcgcggtg ttagacggtg ttgccggagc cagtaccaga
    11941 tcgaggaata ccgtaacgta acccagaccc agcgtggtgt taccgatttt accaatcagc
    12001 agataggcaa tacgtttacc taaaccggtg gtcacaaatg cggcgcttaa ggtaaacgcc
    12061 gagaacacca gccaggtagt acctgaagag taaccgctta atacggcggt ggtcttaaac
    12121 gcaccgtcgg ataagttacc gaccaccacc atggaggcag caacggcaat taacagtacg
    12181 acaggttccg ggaaaggctt gataaccagc cccacaatgg ccgccaggta aataccaaaa
    12241 agcacccacg ccagttcgct taatcctgcc ggagctggca taaaaccaat aatacatggg
    12301 atcgctaata tcagaattag cttccataac gatttcttat tcatttaaaa tcctgttctt
    12361 tctgtatgac tcatacagaa gtcctttatt gattatcctt atcttgacaa cgaatttttt
    12421 cagggaagat aaacttcacc ggaaaatatt tttctggtcg tccgaataac agatgcgcgt
    12481 aacgtcgtgg catcctgacc ttcattactt aaatgaacgt cgagcgcacc actggggtgt
    12541 tcaatattga tattgccgta tcctacagaa gggacgattt gtcgggtgac ggtgccttcc
    12601 aatgcacaac tactggaaat agcaatagca ccggttatcg ccagcgcgcg atggcaagaa
    12661 tgcggcataa aataacgcac attaattgcc ccgcctttct gcgctggaga aataagcaca
    12721 ggtttaggga taaccatatt actgacatca cctaagccca ttgctttacc cgcttgtaga
    12781 cggatagatt caatgcgggc taataatgct ttgtcggcat ccagttccgc cggtaattca
    12841 taacctgttt ttcccagata ttcagccgga ataatgacga ctggcatcgc catatcgata
    12901 caggtcaccg ggacatcgtc aaaataatca atctgattat cagtcgggaa aacttttccg
    12961 gttttggttc cagcggcatt caggaaagtg agcgcaaccg gtgcggcagt acccggtacg
    13021 ccgtcaattc tggcactacc ctcgtactcg acaacaccat ttggcgtttg cacatcagct
    13081 tcgatgaacg tacccgtatt gacgttgcgg atacgcacac gggtgaccgg tgatgtcgct
    13141 gcaatcaaac cattttcaat ggcgaatgcc ccaacgccag aaagcatatt gccgcagtta
    13201 ggcgtggtat cgacacgttg ctcatggacg ataacttgag caaacagata atcaacatca
    13261 gcacgcggat cgctggaacg gctaataatg gcgactttac tggtcagtgg attaccgccg
    13321 ccaataccgt caatttccag atcgttaccg gaacccataa ttgccatcaa tattttatcg
    13381 cgctgcgttt gatcttcggg taaatgttcc gctaacagga acgcgcccct cgaggttcca
    13441 cctcgcatca tcacgcaggg tatttttttc ataataatgc cctcaattta aaaacataac
    13501 ttattgcgat atggttacat taaggacaaa gcatctctaa tgactttagc tcaacgatta
    13561 ataacatttt ttcattaatt taagttggct aaatgcagtt aaaattttta acgaccagcc
    13621 acccaaaatc gtcgcttcca ttttccggag taataatgaa gcatgaatta tcaagtatga
    13681 aggcatttgt catactggca gagtccagtt catttaacaa tgctgctaaa ttactcaata
    13741 ttacgcaacc tgcattaacg cgaagaataa aaaagatgga ggaagattta catattcaac
    13801 tatttgagcg tacaactcgc aaggttacgt taacaaaagc aggaaaaagg ttgctcccgg
    13861 aggcgcggga attaataaag aaattcgatg aaacgctttt taatattcgt gatatgaatg
    13921 cttatcatcg tggtatggtg acgttagcgt gtattccgac cgcagtattt tattttttac
    13981 cgctggcaat cggtaaattt aatgagctgt atcccaatat taaagtgcgg attctggaac
    14041 aaggcacgaa taattgcatg gaatcggtgc tgtgtaacga atcagacttt ggcataaaca
    14101 tgaacaacgt cacaaattca tccattgatt ttaccccact ggtcaacgag ccattcgtgc
    14161 tggcctgtcg gcgtgaccat ccattggcca aaaagcagct tgtagaatgg caggagttag
    14221 tgggttacaa aatgattggc gtacgttcct ccagcggcaa ccgattgttg atagaacaac
    14281 agctagccga caagccctgg aagctggact ggttttacga agtgcgtcat ctttcgacgt
    14341 cattaggact ggtagaggcg ggactgggga tttcggcgct tcctggtctg gcaatgcccc
    14401 atgcgcccta ttcttcgatt attggtattc cactggtgga acccgttata cgtcggacat
    14461 tggggattat tcgccgcaaa gatgccgtgc tttctccggc agcagaacgc ttttttgcct
    14521 tgctgattaa tttgtggact gacgataaag acaatttgtg gaccaatatc gtcgaacgcc
    14581 agagacatgc gttacaggag ataggttaat acaccggcgc aggagagact gcgccggtta
    14641 aatcagcggt tagtgtgcgt taaccaccac ccacattggt ccctgcccga ccgcataacg
    14701 gcctttttca tgcagtagtc cctgctcgcc aacaatttcg tataccgaga tgtggtgaga
    14761 tttttgcccg gcggcaatca gatacttgcc gctgtgatca acattgaagc cgcgcggctg
    14821 ggtttccgtt ggctggaagc cttctttact caacacgctg ccatcttccg aaacgctgaa
    14881 aacggtaatc aggctggcgg tacggtcgca ggcgtataaa tggcgaccat ccggggtgat
    14941 atgaatatca gccgcccaac gggtgtcgga gaagttttcc ggcatcatat ccagcgtctg
    15001 gacacattcg atattaccgt gcggatcttt cagttcccag acatccactg agctgtttaa
    15061 ctcattgacg caatacgcat attgttcgtt tggatggaat accatatgac gcgggccggc
    15121 cccttcaacg gtggtcactt ccgcagggtc ctgcgccacg agatgaccat catcgctgac
    15181 cgtaaacagg caaatgcgat cctgctttaa tgccggaacc cacagcgtac ggttgtccgg
    15241 tgagatattg gcggaatggc aaccgtccag cccctcgacc acatcgacga cgcccactgg
    15301 caggccatct tccagacgcg ttacgctcac gttacccgca ttgtaagaac ctacaaagac
    15361 aaactgcccc tggtgatcgg tggaaatatg cgtcggacta cccggcagcg cagactctgc
    15421 ggcaaaggtc agtgcgccat cgtccggggc gatactatac gccaggacgc gaaactcagg
    15481 gcgaacacca acatagagat aacgtttgtc cgggctgacc accatcggct gcacctgccc
    15541 cggcacatcg acaacctgtg tcagcgtcag tgcgccttca tgattcaggt tccagacgtg
    15601 aatttgctgg ctctcagggc tggcgatata aactgtttgc ttcatgaatg ctcctttgca
    15661 tttagcaact gactgtaaca gctaaaatta gtcgcttttg gcggtgaatg ctgaattaaa
    15721 acgcagaatt ttgtccgata gtggtatcgg tgtaccattc gcgcagacga aaattcttaa
    15781 ccttaatctg gaatacgcca tgaccacacg cgtgattgct ctcgacttag acggcacctt
    15841 attgaccccg aaaaagaccc tgcttccttc atcgatagaa gccctggccc gcgctcgcga
    15901 agcaggctat caattaatca tcgtcacagg tcgccatcac gtcgctattc atccttttta
    15961 tcaggcgctg gcgctggata cacctgctat ttgctgtaat ggcacctatt tgtatgatta
    16021 tcatgcaaaa accgtgctgg aagcggaccc aatgcccgtt aataaagccc tgcaactcat
    16081 tgagatgctg aatgaacacc acattcacgg tctgatgtat gtggatgatg caatggtcta
    16141 tgagcacccg accgggcatg tcattcgcac gtctaactgg gcgcaaaccc tgccgccgga
    16201 acagcgtccg actttcacac aagtcgcttc tctggctgaa acggcgcaac aagttaacgc
    16261 cgtatggaag ttcgccctga cgcacgatga cctgccgcaa ttgcagcatt ttggtaagca
    16321 tgtcgaacat gaactgggac tggagtgtga atggtcctgg cacgatcagg ttgatattgc
    16381 acgcggcggc aacagcaaag gtaaacgttt gacgaaatgg gttgaggcgc aaggctggtc
    16441 gatggaaaac gtcgtggcgt tcggcgataa ctttaatgat atcagtatgc tggaggccgc
    16501 tggtacaggc gtggcgatgg gcaacgccga tgacgcggta aaagcgcgcg ccaacattgt
    16561 gattggtgat aacaccaccg acagcattgc ccagttcatt tatagccacc tgatttaatc
    16621 aggcggttat cgacacactt ttaatttgcg cgtacagcca caggccaggt ttgatcacca
    16681 gttcatccct ggcccacggg ctgatacgcg cccacagcgt tttacctccg acttccagtt
    16741 ccacttccac ctggccgttg tcgtcataac tattaacaac ttttgcccgc aatacgttac
    16801 gaatgctggt ttgctgcggc ggttgtaaaa ccagagaaac atcggaagcc tgaatgcgga
    16861 tacgtagcgc agcttgcagc ggttcgtcca gcttattgac ccacaaatgc tgatcgccca
    16921 gcgccagcgc agtcatcgcg taatgcggat gatgctcaag taccgtcact ttcagaatgc
    16981 tactttgttg ctctttcggc agccacggat tcatcacgct actaccccag acttcttcca
    17041 gtgcgccaaa ggctttcacc tgaccgtttt ccagtaccat cactctgtct gccagatgga
    17101 ggatctcatc cagcgaatgg ctgacataca acatcgggat gttgatttcc cgcgtcagac
    17161 gttgcagata aggcaacagt tcgcgtttac gcggaatatc cagtgacgcc agcggttcat
    17221 ccagcaacag caattctggt gccgtcagca aagcccgccc aatcgctacg cgctgttttt
    17281 cgcctccgga caggctgcct ggtaaacggt caagcaacgg ttcaatgcct aaaagcgcca
    17341 ccagcttatc gaactgatcg accatgcttt tcgccatgcc gtagcgcaga ttgccacgca
    17401 ctttgtaatg cggaaacagc cgcgcatcct gaaaaacata gccaacgcga cgcttttctg
    17461 gcgtcaggca gatacctttt tcggcatcat ttagtacccg accattgagg acaatcctcc
    17521 ctttttgcgg gcgcgtcagt ccactgatgg cgttaatcag cgaagttttt ccggcaccgg
    17581 agacgccaaa gatagcagtg atgccattgg cgggcagcgt ttcattaatg gtcaggcaat
    17641 ggttgcccaa ggtctgggaa aaattcagtt ccagcatgat tagcgccccg cccgttcacg
    17701 gctgattctg gccagccatt ctgaaatcaa cagggagatc atcgccagcg caatagaaat
    17761 aatgcacagt ctcgccgcgc cactttcccc gccgggggtc tggatcaggg tatacatggc
    17821 agaaggaatg gttcgcgttt caccaggaat gttcgacaca aaggtgatgg ttgcaccaaa
    17881 ctcaccgaga gaacgagcaa aagccagtac cgtaccaaca ataattcccg gtaaggtcag
    17941 cggtaacgtg atagtaaaga aaacgcgcca gcgcccggcc cccagtgttc ttgcggcctg
    18001 ttcgagtttg acatcaaccc cttccagcgc cagacgaatt gcccgcacca tcagcggaaa
    18061 cgacatgacg gcggcagcga gaaccgcgcc gcgccagcta aaggcgaagg taataccaaa
    18121 ccagtcatac agacgttcac cgataaatcc gcgccgtccc atcgaaacta ataataagta
    18181 accgacgacc acgggcggta acaccagcgg tagatgcagt acgctgtcga gcagagcttt
    18241 gcccggaaac gtgcaacgca ccagtaacca ggcaaaaaag atcccaaacg gcaggctaaa
    18301 cagcacagcc agggaagaaa ctttcaggct taataaaact gcctgccatt ctggatcggt
    18361 cagtatcatt acttggttgt aaatccgtaa cgtttaaaga tttccgcagc ctgcggccct
    18421 ttcaaataat cataaaatgc tttcactgtc gcattgttat gctcttccac aacagcaacc
    18481 ggatattcca cttttttatg tgaatcttcc gggaaggtgg caaccacttt tacccctttg
    18541 ctggcaactg cgtcagaacc gtaaacaatg cccaacggcg cttcattgcg ttcgaccaac
    18601 gccagcgcgc cacgaacatc ttccgccgga gccagtttcg gggagagcgt atcccatgcg
    18661 cccagttttt gcagtgcttc tttcgcataa atgccagcag ggacatgttc cggatcgcca
    18721 accgcgaggc gaccgccatt cagcagcgaa gtccagttgg ttttgctgtc gatggtgaaa
    18781 tctttctgct cgctggcttt cggtgctaca acgaccaggc tattgccgag cagtgtctgg
    18841 cgcgaagccg tatcgatcgc ttttttatca accgcataat ccatccattt ctgatcggca
    18901 gagataaaca gatccgcagg cgctcccgct tcaatctgac gggccagagt agatgacgaa
    18961 gcgaaagaag aaaccacatc cacgcctttt tcttttttat actgcgtagc aatgtcctgc
    19021 attgcgttag tcagtgatgc ggcggcgaac accgtgattt tcccttcatc tgccagtgta
    19081 ttgccagcaa cagcgaaaga gagtgccgcc ccggcaaaca agttcaacca tttacgagcc
    19141 attggtaacc ccttaatgta acgttatgta ggcgacaata taacggctag ctcagaattt
    19201 tcccagcagt tattgctaac ctttgtgagg tagataagaa aaaatatcgg ctggaaaagc
    19261 aggaagttga gaaaaagaaa tgcccgacta agcgggcatt cagggaatca atgattttgt
    19321 ccgggctggt cttttttacc aacaccagaa aagatgttga atacttcacc aagaccgtaa
    19381 atcagaccca ggatgatggc catcacgaca ggtaccatga ttacggcgaa taccagactt
    19441 tttaataact ctaacatggt caactccaga tatagtcatg agactattct aaccgctaag
    19501 cacagaaaag cactcccctt ttgtgcggtc agctttgcgt ggcgttcgtt ttccgtcaca
    19561 ataagacttt tgccaggaca ttgttatgca ggccgaaatc cttctcaccc ttaagctcca
    19621 acaaaaatta ttcgccgacc cgcgccggat ttcgctacta aaacacattg cgctttccgg
    19681 ttccattagc cagggagcga aagatgccgg tatcagctat aaaagcgcct gggatgccat
    19741 taacgagatg aatcagttaa gtgagcatat tctggtcgag cgcgcaacag gcggtaaagg
    19801 tggcggcggc gcagtactga cccgctatgg tcagcgactg attcagctct atgacttact
    19861 ggcgcaaatc cagcaaaaag cctttgatgt gttaagtgac gatgacgccc tgccgctgaa
    19921 cagcctgctg gccgcgatct cacgtttttc actgcaaacc agcgcccgta accagtggtt
    19981 cggcaccatc accgcccgcg atcatgatga cgttcaacag catgttgatg tcttactggc
    20041 tgacggaaaa acacgcctga aagtcgcaat taccgcacaa agcggcgcgc gtctggggct
    20101 ggatgaaggc aaagaagtgt tgatattgct aaaagcgccg tgggtaggta ttactcagga
    20161 cgaggcggtc gcacaaaacg ccgacaacca attaccgggt attattagtc atattgagcg
    20221 cggcgcagag cagtgcgaag tattaatggc gctacccgac gggcaaacac tgtgcgccac
    20281 agtgccggta aatgaagcga cttctcttca gcaagggcag aatgtcacgg cctactttaa
    20341 tgccgacagc gtgattatcg ccacgctgtg ctaagcgtgt tgacaatttg ttatgaaaca
    20401 cgtatccctg tcagtaatcg ctgcacaaaa tgggatataa aatgtcatcg ttgcaaattt
    20461 tgcaaggcac gtttcgtctt agcgacacaa aaacgctgca attgcctcag ctaacgttaa
    20521 acgcgggtga tagttgggcg tttgtcggtt cgaatggaag cgggaaatcg gccctggccc
    20581 gcgcgctggc gggggaactt ccgcttttga aaggtgaacg gcaaagccag ttttcccaca
    20641 tcactcgtct ctccttcgag caattgcaaa aactcgtcag cgacgaatgg cagcggaata
    20701 acaccgatat gctcggccct ggcgaagatg acaccggacg cactacggct gagatcattc
    20761 aggatgaagt aaaggatgca ccgcgttgca tgcaactggc gcagcagttc ggtattaccg
    20821 ccctcctcga ccgacgcttt aaataccttt ccactggcga gacgcgaaaa accctgctgt
    20881 gtcaggcgct gatgtcggag cctgacttgt tgattcttga tgagccgttc gatggcctgg
    20941 atgttgcctc acgtcagcag ctggctgagc gactcgcctc gttacatcag tccggtatta
    21001 ctctggtact ggtgctcaat cgcttcgatg agatcccgga gtttgtccag tttgctggcg
    21061 tgctggcgga ttgcacgtta gcggaaactg gcgctaaaga ggaactgctc caacaagcac
    21121 tcgtcgcgca actggcgcat agtgaacagc ttgaaggtgt gcaactgccg gagccggatg
    21181 aaccttcagc acgtcacgcc ttacccgcca acgaaccgcg cattgtgctg aacaatggcg
    21241 tggtttctta taacgatcgc cccattctta ataaccttag ctggcaggtg aatccaggtg
    21301 aacactggca aattgtcggg ccaaatggtg caggaaaatc gacgttatta agcctggtta
    21361 ctggcgatca tccgcaaggt tacagcaacg atttgacgct tttcggacga cgtcgcggca
    21421 gcggcgaaac catctgggat atcaaaaagc atatcggtta cgtcagcagt agtttgcatc
    21481 tggattaccg ggtcagcact accgtgcgta atgtgattct ttctggctat tttgattcga
    21541 ttggcattta tcaggccgtt tcggatcgcc agcaaaaact ggtgcagcag tggctggata
    21601 ttctcggcat tgataaacgt acggctgacg ctccgttcca tagtctttcc tggggacagc
    21661 agcgtctggc gctgatcgtc cgcgcactgg tgaaacatcc gacgttgctt attctcgatg
    21721 aaccactaca ggggcttgat ccgctcaatc gccagcttat ccgccgtttt gttgatgtgc
    21781 tgattagcga aggtgaaacg caattgttgt ttgtttcgca ccacgctgaa gatgcgcctg
    21841 cctgtattac ccatcgcctt gagttcgtgc cggacggtgg actctatcgc tatgtgctga
    21901 caaaaatata ttgagtcggt agtgctgacc ttgccggagg cggcctaagc gccctctccg
    21961 gccaacggtt cgacgcatgc aggcatgaaa ccgcttcttt tttcagataa aaagcgcaat
    22021 cattcataaa ccctctgttt tataatcact taatcgcgca taaaaaacgg ctaaattctt
    22081 gtgtaaacga ttccactaat ttattccatg tcacactttt cgcatctttg ttatgctatg
    22141 gttatttcat accataagcc taatggagcg aattatgaga gttctggtta ccggtggtag
    22201 cggttacatt ggaagtcata cctgtgtgca attactgcaa aacggtcatg atgtcatcat
    22261 tcttgataac ctctgtaaca gtaagcgcag cgtactgcct gttatcgagc gtttaggcgg
    22321 caaacatcca acgtttgttg aaggcgatat tcgtaacgaa gcgttgatga ccgagatact
    22381 gcacgatcac gctatcgaca ccgtgatcca cttcgccggg ctgaaagccg tcggcgaatc
    22441 ggtacaaaaa ccgctggaat attacgacaa caatgtcaac ggcactctgc gcctgattag
    22501 cgccatgcgc gccgctaacg tcaaaaactt tatttttagc tcctccgcca ccgtttatgg
    22561 cgatcagccc aaaattccat acgttgaaag cttcccgacc ggcacaccac aaagccctta
    22621 cggcaaaagc aagctgatgg tggaacagat cctcaccgat ctgcaaaaag cccagccgga
    22681 ctggagcatt gccctgctgc gctacttcaa cccggttggc gcgcatccgt cgggcgatat
    22741 gggcgaagat ccgcaaggca ttccgaataa cctgatgcca tacatcgccc aggttgctgt
    22801 aggccgtcgc gactcgctgg cgatttttgg taacgattat ccgaccgaag atggtactgg
    22861 cgtacgcgat tacatccacg taatggatct ggcggacggt cacgtcgtgg cgatggaaaa
    22921 actggcgaac aagccaggcg tacacatcta caacctcggc gctggcgtag gcaacagcgt
    22981 gctggacgtg gttaatgcct tcagcaaagc ctgcggcaaa ccggttaatt atcattttgc
    23041 accgcgtcgc gagggcgacc ttccggccta ctgggcggac gccagcaaag ccgaccgtga
    23101 actgaactgg cgcgtaacgc gcacactcga tgaaatggcg caggacacct ggcactggca
    23161 gtcacgccat ccacagggat atcccgatta aggaacgacc atgacgcaat ttaatcccgt
    23221 tgatcatcca catcgccgct acaacccgct caccgggcaa tggattctgg tttcaccgca
    23281 ccgcgctaag cgcccctggc agggggcgca ggaaacgcca gccaaacagg tgttacctgc
    23341 gcacgatcca gattgcttcc tctgcgcagg taatgtgcgg gtgacaggcg ataaaaaccc
    23401 cgattacacc aggacttacg ttttcactaa tgactttgcg gccttgatgt ctgacacgcc
    23461 agatgcgcca gaaagcaacg atcctttgat gcgttgccag agcgcgcgcg gtaccagccg
    23521 ggtgatctgc ttttcaccgg atcacagtaa aacgttgcca gaactgagcg ttgcggcatt
    23581 gacggaaatc gtcaaaacct ggcaggagca aaccgcagag ctgggaaaaa cgtacccgtg
    23641 ggtgcaggtc tttgaaaaca aaggtgcggc gatgggctgc tctaacccgc atccgcacgg
    23701 acagatttgg gcaaatagct tcctgcctaa cgaagctgag cgcgaagacc gcctgcaaaa
    23761 agaatatttt gccgaacaga aatcaccaat gctggtggat tatgttcagc gcgagctggc
    23821 agacggtagc cgtaccgttg tcgaaaccga acactggtta gccgttgtgc cttactgggc
    23881 tgcctggccg ttcgaaacgc tactgctgcc caaagcccac gttttgcgga tcaccgattt
    23941 gaccgacgcc cagcgcagcg atttggcgct ggcgttgaaa aagctgacca gtcgttatga
    24001 caacctcttc cagtgctcct tcccctactc tatgggctgg cacggcgcac catttaatgg
    24061 cgaagagaat caacactggc agctgcacgc gcacttttat ccgcctctgc tgcgctccgc
    24121 caccgtacgt aaatttatgg ttggttatga aatgctggca gagacccagc gagacctgac
    24181 cgcagaacag gcagcagagc gtttgcgcgc agtcagcgat atccattttc gcgaatccgg
    24241 agtgtaagaa atgagtctga aagaaaaaac acaatctctg tttgccaacg catttggcta
    24301 ccctgccact cacaccattc aggcgcctgg ccgcgtgaat ttgattggtg aacacaccga
    24361 ctacaacgac ggtttcgttc tgccctgcgc gattgattat caaaccgtga tcagctgtgc
    24421 gccacgcgat gaccgtaaag ttcgcgtaat ggcagccgat tatgaaaatc agctcgacga
    24481 gttttccctc aatgcgccca ttgtcgcgca tgaaaactat caatgggcga actacgttcg
    24541 tggcgtggtg aaacatctgc aactgcgtaa caacagcttc ggcggtgtgg acatggtgat
    24601 cagcggcaat gtgccgcagg gtgccgggtt aagttcttcc gcttcactgg aagtcgcggt
    24661 cggaaccgta ttgcagcagc tttattatct gccgctggac ggcgcacaaa tcgcgcttaa
    24721 cggtcaggaa gcagaaaacc agtttgttgg ctgtaactgc gggatcatgg atcagctaat
    24781 ttccgcactc ggcaagaaag atcatgcctt gctgattgac tgtcgctcac tggggaccaa
    24841 agcagtttcc atgccgaaag gtgtggctgt cgtcatcatc aacagtaact tcaaacgtac
    24901 cctggttggc agcgaataca acacccgtcg tgaacagtgc gaaaccggtg cgcgtttctt
    24961 ccagcagcca gccctgcgcg atgtcaccat tgaagagttc aacgctgttg cacatgagct
    25021 ggacccaatc gtggcgaaac gcgtgcggca tatcctgact gaaaacgccc gcaccgttga
    25081 agctgccagc gcgctggagc agggcgacct gaaacgtatg ggcgagttga tggcggagtc
    25141 tcatgcctct atgcgcgatg atttcgaaat caccgtgccg caaattgaca ctctggtaga
    25201 aatcgtcaaa gctgtgattg gcgacaaagg tggcgtacgc atgaccggcg gcggatttgg
    25261 cggctgtatc gtcgcgttga tcccggaaga gctggtgcct gccgtacagc aagctgtcgc
    25321 tgaacaatat gaagcaaaaa caggtattaa agagactttt tacgtttgta aaccatcaca
    25381 aggagcagga cagtgctgaa cgaaactccc gcactggcac ccgatggtca gccgtaccga
    25441 ctgttaactt tgcgtaacaa cgcagggatg gtagtcacgc tgatggactg gggtgcgact
    25501 ttactttcag cccgtattcc gctttccgat ggcagcgtcc gcgaggcgct gctcggctgt
    25561 gccagcccgg aatgctatca ggatcaggcc gcgtttctgg gggcctctat tggtcgttat
    25621 gccaaccgca tcgccaatag ccgttatacc tttgacggtg aaaccgtgac cctttcgcca
    25681 agtcagggcg ttaaccagct gcacggcggc ccggaagggt tcgacaaacg tcgctggcag
    25741 attgtgaacc agaacgatcg tcaggtgctg tttgccctga gttcagatga tggcgatcag
    25801 ggcttcccgg gtaatctcgg cgcaacggtg caatatcgtc tgaccgacga taaccgtatc
    25861 tccattactt atcgcgccac agttgataaa ccttgcccgg tgaatatgac taatcacgtc
    25921 tatttcaatc ttgacggcga gcagtctgac gtgcgcaatc acaagttgca gattctggcg
    25981 gacgaatatc ttccggttga tgaaggcggc attccgcacg acggcttgaa atctgtcgcc
    26041 ggaacgtctt ttgatttccg cagcgccaaa atcatcgcca gtgagtttct tgccgacgac
    26101 gatcagcgca aagtgaaagg ttacgatcac gcattcttgt tacaggccaa aggcgatggc
    26161 aagaaagtgg cggcgcatgt ctggtcagca gatgaaaaat tgcagctgaa ggtctacacc
    26221 accgctccgg ctcttcaatt ctactccggc aacttcctcg gcggcacacc gtcgcgggga
    26281 accgaacctt acgccgactg gcaagggctg gcgttggaaa gcgagtttct gccggacagc
    26341 ccgaaccacc ctgaatggcc gcaaccggac tgcttcctgc gtcctggcga agagtattcc
    26401 agcctgacgg aatatcagtt tattgctgag taatgttaag ccctcgtcat gagggcttta
    26461 tcttcatatt gttcaaatca ccagcaaaca ccgacatatt tgcaactcaa tattcacaac
    26521 aaccttacac tgcgccacta ttttcgctat ggttatgcgt aagcattgct gttgcttcgt
    26581 cacggcaata taatgagaat tattatcatt aaaagatgat ttgaggagta agtatatggc
    26641 tgtaactaag ctggttctgg ttcgtcatgg cgaaagtcag tggaacaaag aaaaccgttt
    26701 caccggttgg tacgacgtgg atctgtctga gaaaggcgta agcgaagcaa aagcagcagg
    26761 taagctgctg aaagaggaag gttacagctt tgactttgct tacacttctg tgctgaaacg
    26821 cgctatccat accctgtgga atgtgctgga cgaactggat caggcatggc tgcccgttga
    26881 gaaatcctgg aaactgaacg aacgtcacta cggtgcgttg cagggtctga acaaagcaga
    26941 aaccgctgaa aagtatggcg acgagcaggt gaaacagtgg cgtcgtggtt ttgcagtcac
    27001 tccgccggaa ctgactaaag atgatgagcg ttatccgggt cacgatccac gttacgcgaa
    27061 gctgagcgag aaagaactgc cgctgacgga aagcctggcg ctgaccattg accgcgtgat
    27121 cccttactgg aatgaaacta ttctaccgcg tatgaagagc ggtgagcgcg tgatcatcgc
    27181 tgcacacggt aactctttac gtgcgctggt gaaatatctt gataacatga gtgaagaaga
    27241 gattcttgag ctgaatatcc cgaccggcgt gccgctggtg tatgagttcg acgagaattt
    27301 caaaccgctg aaacgctatt atctgggtaa tgctgacgag atcgcagcga aagcagcggc
    27361 ggttgctaac cagggtaaag cgaagtaaac gtcattcgtt taaaaatgag aaagccgact
    27421 gcaagtgagc cggctttttt gttgctaaca atggagcaca ttgcctgatg cgacgctgcg
    27481 cgtcttatca ggcctgtggt gattcatcgg atacgccact ctgacggcgc atccgacaat
    27541 taaaccttac ccgcgacgcg cttttactgc attcgccagt tgacgtaaca gagcatcggt
    27601 atcttcccag ccgatgcagg catcggtgat gctcttaccg taggcctgcg gctccccgct
    27661 ctcgaggctc tgattgcctt ccaccagatg gctttccacc atcacgccaa taatggctgt
    27721 ttcgccaccg gcaatctgct ggcaaacgtc agcacacaca tccatctgct ttttgaattg
    27781 tttggacgag ttagcatggc tgaaatcgat catcacctgt gctggcaggc ctgctttgtt
    27841 cagcccttct ttcacttcag caacgtgctt cgcgctgtag ttaggctctt taccgccgcg
    27901 cagaatgata tggcaatcac cgttaccgct ggtattcaca atcgccgaat gcccccattt
    27961 agttacggac aggaagcagt gcggcgcacc ggcggcatta atggcatcga tagccacttt
    28021 aatcgtaccg tctgtgccat ttttaaaacc taccggacaa gaaagaccag acgccagttc
    28081 gcggtgcacc tgcgattcgg tggtacgtgc accaattgcg ccccagctca tcaggtcagc
    28141 gagatattgt ggggtgatca tatcgagaaa ctcacccgcc gctggcagac cgctgtcgtt
    28201 aatatcaagc agcaatttac gggctatacg cagaccgtcg ttgatctgga agctgttatc
    28261 catatgcgga tcgttaatca gccctttcca gcccaccgtg gtacgcggct tttcaaaata
    28321 gacgcgcatt acgatttcca gctcatcttt cagctcttca cgcagcgcca gcaagcgagt
    28381 ggcatactct tttgctgcga caggatcatg aattgagcat gggccaatca ccaccaacag
    28441 gcgatcatca ttacctttca ggatcttatg gatcgctttt cgggcatggg caaccgtatt
    28501 cgcggcattt tcagtagcgg ggaatttttc cagcaatgcg acaggaggaa gtaactcttt
    28561 gatttctttg atgcgtaaat cgtcgttctg ataattcatg tctgttccag tgttgccata
    28621 cttatcttag tgaatgcaat acttccaatc tatatcttcc gtcagaatgt gtaaacgggg
    28681 ttttacacta tgaacggatt aatcctggaa ttagcaagaa aaacgccaga ttgtcgcgaa
    28741 aaacgagatc tctcctacaa tttctaactg taactccttt gtttgttaat tatttcaaga
    28801 ttctctgctg cgtttcataa cctggctgaa atcttaaacc aatgccttat attcacctgc
    28861 aaatgcactg ttggaagagg ttatccgaca taacgaccat aacaggagca tcctatgaaa
    28921 atgacaaaac tggccacatt atttctgact gccactctaa gccttgccag cggtgccgca
    28981 ctggctgccg atagcggagc gcaaactaat aacggccagg caaacgccgc agctgatgcg
    29041 ggccaggtag cccctgacgc ccgtgaaaat gtcgcgccaa ataacgtcga caataacggg
    29101 gtaaataccg gttctggtgg cacaatgctg cattcggatg gttcttcaat gaacaatgac
    29161 ggaatgacca aagatgaaga gcacaagaac accatgtgca aagatggtcg ctgtccggat
    29221 atcaataaaa aggtacaaac cggtgatggc atcaataatg atgtcgatac caaaaccgac
    29281 ggtaccacgc agtaaccctg caaaatagcc aataatggga gagcggagtc tctccctttt
    29341 aatttttcac ttctaaaagc agtatgatgt cataaagtta agtgagaata attaagggat
    29401 gactcgtcat ggcgcactca cactcacaca cgtcctcaca cctgccagaa gataataatg
    29461 ctcgtcgctt gttgtatgct ttcggcgtga ctgccgggtt tatgctggta gaagtcgttg
    29521 gtggttttct ttctggttct ctggcattgc tggccgatgc gggtcatatg ttgaccgata
    29581 ctgccgccct gcttttcgcc ctactcgccg tgcaattttc acgtcgtcct cccaccattc
    29641 gccacacttt cggttggcta agactgacca cgctcgcggc gtttgtgaat gccatagccc
    29701 tcgtggtaat taccattttg attgtctggg aggcgataga acgcttccgc acgccgcgtc
    29761 cggtcgaggg cggcatgatg atggcaattg ccgtggctgg gttgctggca aatatacttt
    29821 ctttctggtt acttcatcac ggcagcgaag agaaaaacct caacgtacga gcagcggcac
    29881 tgcatgtgct cggggacctg ctgggttcgg ttggggctat tattgccgcg ttgattatta
    29941 tctggactgg ctggacacct gctgacccca ttctctcgat actggtgtcg cttctggttc
    30001 tgcgtagcgc gtggcgactg ttgaaagata gcgtgaatga attacttgaa ggtgcaccgg
    30061 tatcgctgga tatcgctgaa ctgaagcgtc gtatgtgccg ggaaatcccg gaagtccgca
    30121 atgtgcacca tgtacatgta tggatggtag gcgagaagcc ggtgatgacg ttgcatgtgc
    30181 aggtgatccc gccacacgat cacgatgcct tgttggatca gatccaacac tacctgatgg
    30241 atcactatca gattgagcac gccaccattc agatggaata tcaaccttgt catgggccag
    30301 actgccacct gaatgaaggg gtgtctggcc attcacatca tcaccattaa tgggacagcg
    30361 cgcgtgagcc tctttcacgt gcgctgttga tccacatccg gctgccgttg agcgcaataa
    30421 aggtcaggat gatgtactcc agagacatgg cgtaaacgcc ctgaagtgca aaaataacga
    30481 cgctaatcac gttaataatc acccacaaca gccagttttc cacatactta cgcgtcatca
    30541 gaatcattgc cacgatagat aacaccatca tgcatgaatc ccagaacggg aaagcgtccg
    30601 gttgcagttc aggcatcaca acctgtaatc ctaatgcttg catgatcatg actgccaggc
    30661 gggtcaaaaa tgcaaacacc ggattgataa ataccgtcat cagaccaatc gaaacaacgc
    30721 aaaccgccaa ccagctgagt gccttcggca atggcaacca gcgaattttc aactccgcct
    30781 cgttctgact ggtttgtcgc gaccacgcat accaaccgta aatattcgcg gcaaagaaaa
    30841 acacctgtaa tagcaggctg gcatacagct gaatctgaaa gaaaataatg ccaaacaagg
    30901 tgacgttgat caggccaaag aagtagttgc tgatcttctc cagactggca aggccaatac
    30961 acagcaaccc ggcgatcgtg cctaccgctt cgatccatga gagatcataa ccgcctgccc
    31021 ctattggtat atgtaccagg atattctgca cactaaaaaa atccatcttt cccccaaagc
    31081 gtaacgttac gccttattat tcgttatcca cgtagtgtag ccgcaaaatc cagcatacga
    31141 ttgagcggca ccagcgccct ctctcgcagc ctttcatcaa catgaacctc gtgattgctt
    31201 ccttcctgtt ctaatgcctc tgcgatggac tgaaggccat tcatggccat ccacggacaa
    31261 tgcgcgcagc tgcggcaggt tgcaccctca cctgcggttg gtgcttccag taactcttta
    31321 tctggcactg cctgctgcat tttgtagaaa atgccccgat cggttgccac gataagccgc
    31381 tgatgtggca atgttttcgc agcagcgatc agttgactgg tggaaccgac cgcatccgcc
    31441 atatcgacaa tagcttgtgg tgattctgga tgcaccagta tggcagcatc cgggtattct
    31501 tcttgcaagc gggttaacgc ctgagtctta aattcatcat gcacaataca ggcaccctgc
    31561 cagcatagaa tgtctccacc cgtctgtttt tgcacgtaac gccccagatg tttgtcgggt
    31621 gcccagatga ttttttcacc caaactatca agatgatcaa taagttcgac ggcaatgctt
    31681 gaagttacca cccaatctgc gcgcgctttt accgcagcag aagtgttggc gtagacgacg
    31741 acagtacgat cgggatgggc atcgcaaaat gcgttaaatt cttcaacagg gcagccgaga
    31801 tccagtgaac attcagcctg aagtgtcggc atcagaattg ttttttccgg actgagaatt
    31861 ttggcggttt ctcccataaa tctcacccca gcgactaaca aagtagaagc gggatgcttt
    31921 gcaccgaagc gcgccatttc cagagaatca gaaatacagc caccggtttc ttctgccagt
    31981 tgttgaattt cgggatcggt atagtagtgg gcaaccatca ccgcattacg ttcttttagc
    32041 agacgtttta tcttctcgcg gtaatacgct ttttcatcaa tgcttaacgg cgtcggcttc
    32101 ggggggaaag gataaatcgc cgtgtctgga tcaaacatta cgctcatctt accatctcgt
    32161 tttacaggct taacgttaaa accgacatta gcgtaatatt cgctgttatc ggtgtaattt
    32221 gttttatatg ctaaacaaga tagtcgaatg gtggggaaaa gtcactcgaa ttacgcctaa
    32281 ttccaccgac gtagagttgc ttgcacagta agctctgacg atgtcatcag gtttacaaag
    32341 tgaaattgag gatgcctgaa tagcaaaaaa gcgcctttag ggcgcttttt tacattggtg
    32401 ggtcgtgcag gatgactcgc ttcgctcgcc cttcgggccg tcgccgcgag cggctccgtt
    32461 gtttcgctgc gctcaactca aacctgctgc aggttcgaat ctttaagtat cgcagatagc
    32521 aaaaaagcgc ctttagggcg cttttttaca ttggtgggtc gtgcaggatt cgaacctgcg
    32581 accaattgat taaaagtcaa ctgctctacc aactgagcta acgacccctt gcgggattat
    32641 gctgctttaa tcattcagga tggtgggtcg tgcaggattc gaacctgcga ccaattgatt
    32701 aaaagtcaac tgctctacca actgagctaa cgacccatac gggtgttacc tgaaagattt
    32761 ctactcgggg ccaatcttta agattggtgt gtcgtgcagg atgactcgct tcgcttgccc
    32821 tacgggccgt tgctggcgca acgttatcct tcacgcttaa catctgagtt tgatgttaaa
    32881 attggtgggt cgtgcaggat tcgaacctgc gaccaattga ttaaaagtca actgctctac
    32941 caactgagct aacgacccga gtggtgggtg atgacgggat cgaaccgccg accccctcct
    33001 tgtaagggag gtgctctccc agctgagcta atcacccgat actgcgctgg atactacaaa
    33061 ctacttccac cgaacccacc gtaattagtg gtgggtcgtg caggatgact cgcttcgctc
    33121 gccctacggg ccgttgctga cgcaacgtta tcctacacgc ttaacatctg tcttcgatgc
    33181 taaaactggt gggtcgtgca ggattcgaac ctgcgaccaa ttgattaaaa gtcaactgct
    33241 ctaccaactg agctaacgac ccggtggtgg gtgatgacgg gatcgaaccg ccgaccccct
    33301 ccttgtaagg gagatgctct cccagctgag ctaatcaccc aatactacgc tggatactac
    33361 aactaactat gttagtggtg ggtcgtgcag gatgactcga cttcgtctcg cccttcgggc
    33421 cgttgctgat gcaacgttat ccttcacgtt ttactaccgc ttccacctta gcgattggtg
    33481 ggtcgtgcag gattcgaacc tgcgaccaat tgattaaaag tcaactgctc taccaactga
    33541 gctaacgacc cactttttcg ttgctttcgg tttgtttgat atcccgtggc aacggcggca
    33601 tatattactg atttcagact tgagcgcaac aaaaatttcg atgcagatca ctcaactgct
    33661 tatgattcgc acgacacgac cagaaataat gcgacttctg gtcgtgtgtt atgcattaca
    33721 tcgcgttcag acgtttttgt gcctgtttag cgccatcagt accagggtat ttactgataa
    33781 cctgctggta tacggctttc gcttttgcgg tgtcaccttt gtcctgcatg atgacgccga
    33841 ctttaaacat cgcatcagca gcctttggtg acttcggata gtttttcact accgaagcaa
    33901 aatagtacgc cgcatcatct tttttaccct tgttgtagtt taactgaccc agccaataat
    33961 tggcgtttgg caggtaagtt gaatcagggt aatttttgat gaaattctga aatgccacca
    34021 ttgcgtcatc ctggcgggat ttatcctgca ccagcgcaat agctgcattg taatccgtgt
    34081 ttgcatcgcc gcttttcacc ggcgcgccag cattcgcagt accagcatca gctgtcggcg
    34141 tcgttgatgc cgccgcaccg ctttgatcgc tgctggttga ttgcgccgct gcaccaccgc
    34201 tgctgaggct gtcgatctgc aacaggatct gcttttgccg ctccacgacc tgattcagtt
    34261 gatactgatt ttcctgaatc tgaccacgca gggaatcaat atcggattga ttatcagaaa
    34321 gttgttgctg gagttgggtt aaaagctggc tgtgagcatt agaaatacgc tcaagttgag
    34381 tgacgcggtc ttcgaccgag cctgagccga cactactgat tggtgcctga gcaaaagcgg
    34441 cccagggggc cgctatacca accagtaacg acagactcaa tagttgatgt ctgaagttac
    34501 tgctcatgca attctcttag taaaccagta ccgcacgacg gtttttggag tatgccgctt
    34561 cgtcatgacc cagtactgca ggtttttctt taccgtaaga aacgatggag atctggtctg
    34621 cagaaacgcc tttaccctgc aggtacatct taacggcgtt cgcacgacgt tcacccaggg
    34681 agatgttgta ttccggagta ccacgttcgt ccgcgtgacc ttctacggtg actttgtaag
    34741 acgggttgct acgcaggaag tttgcatgtg catccagcat ttgagcgaag tcagaacgga
    34801 tatcgtactt gtccagatcg aagtaaacga tgttgttctg ctgcagctgt tgcatttgca
    34861 gacgagcctg ctcttcggaa gacatgttgc cgttgccgcc gttcgcatcc ataccagtgc
    34921 cggcacccag catgccttcg ctgccgtcat tgctggcgtt cttgttggaa gaacatgccg
    34981 caattgccat aacaggcaga gcaatcatca gccctttcag cactttgttc agttgcattt
    35041 caataattcc tttactattc aattaattat tatcacagat acggcgacca ggcagggaat
    35101 ttgacctgtc catcagttgc cggaagacgc gctttgaaac gcccatctgt agaaaccaaa
    35161 ttcagcacgg atcccatccc ctgagaagag ctgtagatta ccatagtgcc gttaggtgcc
    35221 agacttggcg tttcatccag gaacgtggac gacagaactt gtacgcctcc cgttgccaga
    35281 tcttgtttgg caatgtgctg ctgcccacca ttggagctga ccattaccat aaatttaccg
    35341 tcgctgctga catccgcatc ctggttctgc gaaccttccc aggtaatacg ttgtggcgca
    35401 ccgccgttga tattcacttt ataaacctgc ggacgaccgg cctggtcaga agtaaatgcc
    35461 aggttctggc tatccgggaa ccaggtcggt tcggtattgt tactgcgacc atcagtcacc
    35521 tggcggatct gaccagaagc caaatccatt acgtacaggt tcagactacc ggttttcgac
    35581 aaggcgaatg ccagtttgct gccgtctggc gagaatgcag gtgcaccgtt gtgacgcggg
    35641 aatgaagcca cctgacgtac agcgccattt gccagcgtct gaataaccag cgcggaacga
    35701 ccgctttcga aggtcacata agccagttta gaaccgtctg gtgaccacgc cggagacatc
    35761 agcggctgcg gtgaacggtg aacgacaaac tggttgtaac cgtcatagtc agatacgcgc
    35821 agttcatacg ggaactgacc gccgttggtc tgaacaacgt aggcaatacg agtacggaac
    35881 gcacctttaa tgccggtcag tttttcaaac acttcatcac tggcggtatg accagcataa
    35941 cgcagccact gcttgttcac tttgtacgag ttctgagcaa gtacagtacc cggtgcgccg
    36001 ccagtgtcaa caagttgata agcaacattg taagaaccat ccggattcgg agtgacctga
    36061 ccgacaacta cagcgtcaat gcccagtgcg gaccatgcag ctggttgtac ttcctgcgca
    36121 ctacccggct gctgtggcag acgagcgcga tctaacggat taaatttacc gctgttacgc
    36181 aagtcagcag caacgatgcc gccaatatct tcaggtgccg caccaggccc cgcccactgg
    36241 aaaggaacaa caccaatagg acgaccggaa tctacaccgc tgtcgatcac aatgcggact
    36301 tcagcatgca gaactgatgc ccacagtatg agaaaaccaa atgctactcg taatgcctgc
    36361 ttcatcatat ctcccttatc tggaccgatg gcccacgata atttagcaga atgttaacaa
    36421 actaaaatac acaaaactac cagaaccccg tggcaaccgg tgcctgatgt tgaccgtccg
    36481 aacagtcaac atcgcgatta cggtttgaag tccaatggcg cgtttttgaa cacttcatat
    36541 actgcctggc ttggtggttt cgggatcttc gcaagtttag ctgctgccaa cgcagcctga
    36601 caaagtgcgg gatcgccacc ttcaggtttg atatccagta acataccatc gggtgccagt
    36661 tttatgcgca gcgtacaggt tttgcctgca taggacgatg cgtcatagaa cttactttcg
    36721 atagcagatt taatctgccc ggcatagtta ttgatatcgg cccctgatgc gccattgttt
    36781 ttagtattac cactcccggc aggcgaagca ttgttccctt tcgcccctcc ccccgttttc
    36841 ggtgcattct taccagagct tagctcaccg aaaatatcat ctgcctctgc ggccgctttt
    36901 gctgcagcgg ctttttcagc tgcagctttt gccgctgccg cttttttgtc tgcggcggct
    36961 ttttctgccg ctgctttctt atcagctgct gccttttcag cagccgcttt ttctgctgct
    37021 tttttgtcgg ctgcagcttt ctctgctgcc gctttcttat cagctgcagc cttttcagca
    37081 gccgctttct tctcagcttc tgctttggct ttttcagcag cttcagttgc cgctttcttt
    37141 cttgcttcag ctgcagctgc ttctgccgct tccgctttct tcttcagtgc cgcagcggct
    37201 gcctcggctt ttttctgcgc ttcggctgcg gctttggcgg cttctgcttc tgcttttttc
    37261 tttgcgtctg cagccgcttt cttcgctgct tcttccgcag ctttagcatc tgcttcggcc
    37321 ttcgctttag catctgccgc cgctttcgct gccgcctctt cagcttgctt ctgctttaac
    37381 tcggcctgtt ttgcggcttc ttcagcctgc tttttctgct cctgagccgc taaccgctct
    37441 ttctcaagtt gcttcaggcg ttcctgttca gccgcttgtt tctcacgcag ttcttcagca
    37501 gcctgctgtt ccttcatctt gcgctgttca tcagaacgct tcgcgcttga ttcctggctt
    37561 tgcatgcgtt tgtactgctc aactaccgca cctgaatcaa ccatgacagc gtcgatggac
    37621 gaaccaccgc cgcctccggc tgaagcttct atattctcat cgaacgaact ccagatcagc
    37681 gccgcaaata agatgacatg cagcactgct gaaataatta tcgcccgctt gagcttgtcg
    37741 ttttgttcgg ttgcctttga cactctcggt ttccaaaaac tgttcgcctg ttacccgctc
    37801 tctttcaagc aagggaaacg cagatgttta gataggctgc gtcattaaac caaccgattt
    37861 cacacccgca ctatgtaaca agttcagtgc tttaattatt tcatcgtaag gcacatcttt
    37921 tgcgccaccg atcagaaaga ccgttttcgg gttggccttg aaacggctgg acacttccgc
    37981 caccacctgc tctggtggta aacgctccag gcgatctttc tcaaccacca cggtgtactg
    38041 accaatacca gacacttcaa caatcactgg cggattatcg ttactgctca ccgcctgtga
    38101 ttcagtagcg tctggcagat cgacctccac gctctgggtg atgatgggcg ctgtcgccat
    38161 gaagatcagc aacagcacca gcagtacgtc cagcaacggt acaatgttga tttcggactt
    38221 aagttcgcga cgacctcgtc cacgcgctct ggccatgact taccccttat tgctctcgct
    38281 aacggtaaac gcctggcggt gcagaatcgc ggtaaactct tccataaagt tgtcgtaatt
    38341 cagttccagt ttgtttacgc gctggttgag gcggttgtag gccataactg ccgggatagc
    38401 ggcaaacaga ccaattgcag tcgcaatcaa cgcttctgcg ataccgggcg caaccatttg
    38461 cagtgttgct tgttttaccg ccccgagggc gataaaggcg tgcatgatcc cccagaccgt
    38521 accaaacaga ccaatatacg ggctgatgga gccaaccgta ccgaggaacg gaatgtgcgt
    38581 ttccagattt tcaagttcac ggttcatgga gatacgcata gcacgcgacg ccccttccac
    38641 tacggcttcc ggcgcatggc tattggcacg atgcaggcgc acaaactctt tgaacccgct
    38701 gtaaaatatt tgttccgaac cagtcagatt atcccgtttc ccctgactct cttgatagag
    38761 gcgagagagt tcgattccag accagaattt atcttcaaac gcttcggctt cgcgcgccgc
    38821 tgcgttaaga atacgggtcc gctggataat aatggcccag gatgcgattg aaaaaccaat
    38881 caaaatcaac atgataagtt taaccagaag gctagccttc aggaacaaat caaggatatt
    38941 catgtcagtc actgcttaaa ctccgcgaca atagacttgg gaagcgcacg aggcttcatt
    39001 ttgagtgggt caacgcaaac aaccagaacc tctgcttcat tcagcaaagt attctcggcg
    39061 ttgacaatac gttgcgtgaa aaccaaagag gtgccacgca ttgatgttat ttcagtctgt
    39121 atttcgagca tatcgtcgag ccgcgcaggt gcgtaatatt ccaccgtcat tttacgtacc
    39181 acaaaggcaa cgcgttcagc catcagcgcc tgctgactga agtgatgatg acgcagcatc
    39241 tctgtgcgtg ctctttcata aaaagcgacg taactggcgt ggtacaccac accaccggca
    39301 tcggtatctt catagtagac gcgaaccggc catcgaaaca gcgttgtatt cactttacat
    39361 cccggtaatg caacaaaagt tagagctttt aaacgctgct actatacgcg cgcgattgag
    39421 gtttgggaat gagggcaagt taagggagcg taaaaattta tgggcctgga gcggcccata
    39481 attttttaaa gagaattctg atttaaaaga agaagaaaat cagcccgaca atgagaacaa
    39541 tatcggcaag caatgggcaa aaaatccctt gccaaagaac cttctgcgga cgaaagccca
    39601 cgccgtgaat cacaccagca cagacggccc acatcagcaa taaaccatgc cagatttcca
    39661 gttcactggt cttcgcggca aaacgagatg ggtcccaaaa catacatcct gctaacagaa
    39721 gcgccatcac gaaggaaagc gcccgcaggg ggcgcttgtc cattaccgca tacaaagtcg
    39781 cgataatctt actcatcaga tgtcttcttg accggctttg cctgattcga cgtgttcaag
    39841 cgccagcgcg gtgattaccc caaacgaaca ggcaagaagc gttcccagaa tccatgcgaa
    39901 ataccacatt tttagctcct tacttagtac agagagtggg tgttacgttc aatatcttct
    39961 ttggtgatac gaccgaacat tttccagtaa caccaggcgg tgtagagcag aatgatcggt
    40021 accagaacca ccgcaaccca ggtcatgacg ttaagcgtca gctggctgga agttgcatcc
    40081 cacattgtca gacttgcgtt catcatggtg ctggacggca tcacaaacgg gaacattgcg
    40141 ataccggctg tcaggatgat gcaggccagc gtcagggagg agaacacaaa cgcccacgcg
    40201 gctttatcca tacgtgcagt caggatggtc agcagcggca gaaccacacc cagtgccgga
    40261 atagcccaca gaattggcgt gttgttgaag ttaaccagcc atgcgccagc ttcacgaacc
    40321 acttctttat tcagtgggtt agaggctgcg taatggtcca ttgtcgattt cacgacataa
    40381 ccatcgatac cgtacatcac ccatacgcca gccagtgcga aacagaccag tgtcaccagc
    40441 gcagccacct gagccgttgc acgggtacgc aggtgcagtt cgcccacggt acgcatttgc
    40501 agataggttg cgccctgagt aatgatcatc cctacgctca ccacgcctgc cagcaggccg
    40561 aacgggttaa gcaactggaa gaagttaccg gtgtagtaca gacgcagata ttcatcaacg
    40621 ttgaacggta cgccctgcaa caggttaccg aacgctacac caattaccag cggcggaacg
    40681 aagctaccaa tgaagatgcc ccagtcccac atgttacgcc agcgggtttc ttcaatcttg
    40741 gagcggtagt caaaaccgac cggacggaag aacaaagacg ccagcacgag gatcatcgcc
    40801 acatagaagc cggagaacgc agcggcatag accatcggcc aggcagcaaa gagtgcgccg
    40861 cccgcggtga tcagccaaac ctggttaccg tcccagtgtg gtgcaatgga gttaatcata
    40921 attcgacgct cggtgtcgtt acgaccgagg aaacgggtga gcatgcccac ccccatgtcg
    40981 aaaccgtcag tgactgcaaa accaatcagc agaacgccaa ccagcagcca ccagataaaa
    41041 cgcaatactt cataatcgat catttgacga ctcctgtctt agcgtgccgg ctgagtagtc
    41101 gtggaagact gctcaaagtg atagcgaccg gttttcaggc tgcttgggcc gaggcgtgca
    41161 aatttgaaca ttaagaacaa ttctgccacc aggaacaggg tatacaggcc gcaaatcagc
    41221 accattgaga agatgagatc gcctgcggtc agtgacgagt tcgccacagc tgtcggcagc
    41281 acttcaccga tagcccacgg ttggcggcca tattcagcca cgaaccagcc cgcttctaca
    41341 gcaatccacg gcagcggaat accgtacagc gcggcgcgca gaagccattt tttctcgcca
    41401 atgcggttgc ggatgacact ccagaaagag agcgcgatga ttgccagaag caggaagcca
    41461 cacgccacca tgatacggaa cgcaaagtac agcggcgcta cacgcgggat ggagtctttg
    41521 gttgcctgtt gaatctgcgc ttcagtcgca tcagccacgt ttggcgtata gcgtttcagc
    41581 agcagaccgt aaccgaggtc tttcttcatg ctattgaact ggtcacgaac cgcctggtcg
    41641 gtagaaccag aacgcagttg ttcgagcaga gagtacgcct tcatcccgtt acgaatgcgt
    41701 tcttcatgct gcaccatcag ctctttcagg ccgataaccg gggtatccac ggaacgcgtt
    41761 gcaatgatgc ccagtgcgta agggatctga atcgcaaatt tgttcgtctc ctcttcctga
    41821 tcaggaatgc cgaacagagt aaaggcagca ggcgcaggtt gcgtttccca ctcggcttca
    41881 atagcagcca gtttggtttt ctgcacgtcg cccatttcgt agccggattc atcacccaga
    41941 acaataacag acagaacagc agccataccg aagctggcag cgatagcaaa ggagcgttta
    42001 gcgaaggcga agtcacgacc tttcagcata taccatgcgc tgataccgag gatgaacatc
    42061 gcgccagtca cataaccaga cgctacagtg tgaacgaatt tcacctgagc aaccgggtta
    42121 agcaccagct cggagaagct caccatctcc atacgcatag tttcaaagtt gaaatcggac
    42181 gcgattgggt tttgcatcca gccgttcgca accagaatcc acagtgcgga caggtttgaa
    42241 ccgagcgcca ccagccaggt gacacacata tgctgaactt tacccagacg atcccaaccg
    42301 aagaagaaca gacctacaaa ggtggattcg aggaagaagg ccatcagacc ttcgattgcc
    42361 agcggcgcac cgaagatatc ccctacatag tgggaatagt aagaccagtt agtcccgaac
    42421 tggaactcca tggtcagacc ggtagccaca cccagagcga agttgatacc aaacaacttg
    42481 ccccagaact tggtcatatc tttataaatc tgtttgccgg agaggacgta gaccgtttcc
    42541 ataatggcca gcaggaacgc cataccgagc gtcagtggca caaaaaggaa gtggtacatc
    42601 gcggtcaagg caaactgtaa gcgcgacagt tcgactatat ctaacatcat gactccttgc
    42661 tcatcgcatg aagactccga gagtgaaccc cgttagaaag ggtcacacgc atgccccaat
    42721 agaaatttgt ttgaacactt tcatcatacc atgtgaatgc acacaaaaac ggcgtaaaga
    42781 aaggttgcaa acatgttaat aaaaactcaa attgatccca cgtatatatt acgccgcaaa
    42841 atccttacaa taaacaggtt tttgttgaag caaatttgca tttttcgacg gtgatcaatt
    42901 tatagctaaa ttaccgcctt tcagccaatt tgatcgagaa caatttatct ctttttgat
//
LOCUS       DAZKHF020000011        42218 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2201S31241
            SAMN43267800-rid25106413.denovo.011, whole genome shotgun sequence.
ACCESSION   DAZKHF020000011 DAZKHF020000000
VERSION     DAZKHF020000011.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267800
            Sequence Read Archive: SRR30307263
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 42218)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 42218)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 42218)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350581.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350581.2
            Genome Coverage       :: 151x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:21:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,652
            CDSs (total)                      :: 5,557
            Genes (coding)                    :: 5,320
            CDSs (with protein)               :: 5,320
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 237
            CDSs (without protein)            :: 237
            Pseudo Genes (ambiguous residues) :: 0 of 237
            Pseudo Genes (frameshifted)       :: 102 of 237
            Pseudo Genes (incomplete)         :: 130 of 237
            Pseudo Genes (internal stop)      :: 52 of 237
            Pseudo Genes (multiple problems)  :: 41 of 237
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..42218
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267800-rid25106413.denovo.011"
                     /serotype="O 26:H11"
                     /isolate="2201S31241"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-01"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            182..808
                     /locus_tag="ACHOSC_RS02405"
                     /old_locus_tag="pgaptmp_000481"
     CDS             182..808
                     /locus_tag="ACHOSC_RS02405"
                     /old_locus_tag="pgaptmp_000481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin-like protein"
                     /protein_id="HGU4546485.1"
                     /translation="MNPVDRPLLDIGLTRLEFLRISGKGLAGLTIAPALLSLLGCKQE
                     DIDSGTVGLINTPKGVLVTQRARCTGCHRCEISCTNFNDGSVGTFFSRIKIHRNYFFG
                     DNGVGSGGGLYGDLNYTADTCRQCKEPQCMNVCPIGAITWQQKEGCITVDHKRCIGCS
                     ACTTACPWMMATVNTESKKSSKCVLCGECANACPTGALKIIEWKDITV"
     gene            829..2931
                     /locus_tag="ACHOSC_RS02410"
                     /old_locus_tag="pgaptmp_000482"
     CDS             829..2931
                     /locus_tag="ACHOSC_RS02410"
                     /old_locus_tag="pgaptmp_000482"
                     /EC_number="1.2.7.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016246021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde ferredoxin oxidoreductase"
                     /protein_id="HGU4546486.1"
                     /translation="MANGWTGNILRVNLTTGNITLEDSSKFKSFVGGMGFGYKIMYDE
                     VPPGTKPFDEANKLVFATGPLTGSGAPCSSRVNITSLSTFTKGNLVVDAHMGGFFAAQ
                     MKFAGYDVIIIEGKAKSPVWLKIKDDKVSLEKADFLWGKGTRATTEEICRLTSPETCV
                     AAIGQAGENLVPLSGMLNSRNHSGGAGTGAIMGSKNLKAIAVEGTKGVNIADRQEMKR
                     LNDYMMTELIGANNNHVVPSTPQSWAEYSDPKSRWTARKGLFWGAAEGGPIETGEIPP
                     GNQNTVGFRTYKSVFDLGPAAEKYTVKMSGCHSCPIRCMTQMNIPRVKEFGVPSTGGN
                     TCVANFVHTTIFPNGPKDFEDKDDGRVIGNLVGLNLFDDYGLWCNYGQLHRDFTYCYS
                     KGVFKRVLPAEEYAEIHWDQLEAGDVNFIKDFYYRLAHRVGELSHLADGSYAIAERWN
                     LGEEYWGYAKNKLWSPFGYPVHHANEASAQVGSIVNCMFNRDCMTHTHINFIGSGLPL
                     KLQREVAKELFGSEDAYDETKNYTPINDAKIKYAKWSLLRVCLHNAVTLCNWVWPMTV
                     SPLKSRNYRGDLALEAKFFKAITGEEMTQEKLDLAAERIFTLHRAYTVKLMQTKDMRN
                     EHDLICSWVFDKDPQIPVFTEGTDKMDRDDMHASLTMFYKEMGWDPQLGCPTRETLQR
                     LGLEDIAADLAAHNLLPV"
     gene            2944..3582
                     /locus_tag="ACHOSC_RS02415"
                     /old_locus_tag="pgaptmp_000483"
     CDS             2944..3582
                     /locus_tag="ACHOSC_RS02415"
                     /old_locus_tag="pgaptmp_000483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707570.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdhW family putative oxidoreductase system
                     protein"
                     /protein_id="HGU4546487.1"
                     /translation="MNHRDELPLAKVSEVDEAKRQWLQGMRHPVDTVTEPEPAEILAE
                     FIRQHSAAGQLVARAVFLSPPYSVAEEELSVLLESIKQNGDYADIACMTGSQDDYYYS
                     TQAMSENYAAMSLQVVEQDICRAIAHAVRFECQTYPRPYKVAMLMQAPYYFQEAQIEA
                     AIAAMDVAPEYADIRQVESSTAVLYLFSERFMTYGKAYGLCEWFEVEQFQNP"
     gene            3646..4314
                     /locus_tag="ACHOSC_RS02420"
                     /old_locus_tag="pgaptmp_000484"
     CDS             3646..4314
                     /locus_tag="ACHOSC_RS02420"
                     /old_locus_tag="pgaptmp_000484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416186.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HGU4546488.1"
                     /translation="MSFTRRKFVLGMGTVIFFTGSASSLLANTRQEKEVRYAMIHDES
                     RCNGCNICARACRKTNHVPAQGSRLSIAHIPVTDNDNETQYHFFRQSCQHCEDAPCID
                     VCPTGASWRDEQGIVRVEKSQCIGCSYCIGACPYQVRYLNPVTKVADKCDFCAESRLA
                     KGFPPICVSACPEHALIFGREDSPEIQAWLQQNKYYQYQLPGAGKPHLYRRFGQHLIK
                     KENV"
     gene            4311..5096
                     /gene="phsC"
                     /locus_tag="ACHOSC_RS02425"
                     /old_locus_tag="pgaptmp_000485"
     CDS             4311..5096
                     /gene="phsC"
                     /locus_tag="ACHOSC_RS02425"
                     /old_locus_tag="pgaptmp_000485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiosulfate reductase cytochrome B subunit"
                     /protein_id="HGU4546489.1"
                     /translation="MNPSQHAEQFQSQLANYVPQFTPEFWPVWLIIAGVLLVGMWLVL
                     GLHALLRARGVKKSVTDYGEKIYLYCKAVRLWHWSNALLFVLLLASGLINHFALVGAT
                     AVKSLVAVHEVCGFLLLACWLGFVLINAVGGNGHHYRIRRQGWLERAAKQTRFYLFGI
                     MQGEEHPFPATTQSKFNPLQQVAYVGVMYGLLPLLLLTGLLCLYPQAVGDVFPGVRYW
                     LLQAHFALAFISLFFIFGHLYLCTTGRTPHETFKSMVDGYHRH"
     gene            5100..5912
                     /gene="ydhT"
                     /locus_tag="ACHOSC_RS02430"
                     /old_locus_tag="pgaptmp_000486"
     CDS             5100..5912
                     /gene="ydhT"
                     /locus_tag="ACHOSC_RS02430"
                     /old_locus_tag="pgaptmp_000486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YdhT"
                     /protein_id="HGU4546490.1"
                     /translation="MIITRADLREWRIGAVMYRWFLRHFPRGGSYADIHHALIEEGYT
                     DWAESLVEYAWKKWLADENFAHQEVSSMQKLATDPGEIPFCSQFARSDDHARIGCCED
                     NARIATAGYAAQIASMGYSVRIGSVGFNSHIGSSGERARVAVTGNSSRISSAGDSSRI
                     ANTGMRVRVCTLGERCHVASNGDLVQIASFGANARIANSGDNVHIIASGENSTVVSTG
                     VVDSIILGPGGSAALAYHDGERVRFAVAIEGENNIRTGVRYRLNEQHQFVEC"
     gene            complement(5924..7529)
                     /locus_tag="ACHOSC_RS02435"
                     /old_locus_tag="pgaptmp_000487"
                     /pseudo
     CDS             complement(5924..7529)
                     /locus_tag="ACHOSC_RS02435"
                     /old_locus_tag="pgaptmp_000487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416183.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-NAD(P)-binding protein"
     gene            complement(7655..7960)
                     /locus_tag="ACHOSC_RS02440"
                     /old_locus_tag="pgaptmp_000488"
     CDS             complement(7655..7960)
                     /locus_tag="ACHOSC_RS02440"
                     /old_locus_tag="pgaptmp_000488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="monooxygenase"
                     /protein_id="HGU4546491.1"
                     /translation="MATLLQLHFAFNGPFGDAMAEQLKPLAESINQEPGFLWKVWTES
                     EKNHEAGGIYLFTDEKSALAYLEKHTARLKNLGVEEVVAKVFDVNEPLSQINQAKLA"
     gene            complement(8068..8144)
                     /locus_tag="ACHOSC_RS02445"
                     /old_locus_tag="pgaptmp_000489"
     tRNA            complement(8068..8144)
                     /locus_tag="ACHOSC_RS02445"
                     /old_locus_tag="pgaptmp_000489"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(8108..8110),aa:Val,seq:gac)
     gene            complement(8149..8225)
                     /locus_tag="ACHOSC_RS02450"
                     /old_locus_tag="pgaptmp_000490"
     tRNA            complement(8149..8225)
                     /locus_tag="ACHOSC_RS02450"
                     /old_locus_tag="pgaptmp_000490"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(8189..8191),aa:Val,seq:gac)
     gene            8533..9789
                     /locus_tag="ACHOSC_RS02455"
                     /old_locus_tag="pgaptmp_000491"
     CDS             8533..9789
                     /locus_tag="ACHOSC_RS02455"
                     /old_locus_tag="pgaptmp_000491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AIDA repeat-containing protein"
                     /protein_id="HGU4546492.1"
                     /translation="MGSDAKNLMSDGNVQIVKTGEVIGATQLTEGELIVEAGGRAENT
                     VVTGAGWLKVATGGIAKCTQYGNNGTLSVSDGAIATDIVQSEGGAISLSTLATVNGRH
                     PEGEFSVDQGYACGLLLENGGNLRVLEGHRAEKIILDQEGGLLVNGTTSAVVVDEGGE
                     LLVYPGGEASNCEINQGGVFMLAGKASDTLLAGGTMNNLGGEDSDTIVENGSIYRLGT
                     DGLQLYSSGKTQNLSVNVGGRAEVHAGTLENAVIQGGTVILLSPTSADENFVVEEDRA
                     PVELTGSVALLDGASMIIGYGADLQQSTITVQQGGVLILDGSTVKGDSVTFSVGNINL
                     NGGKLWLITGAATHVQLKVKRLRGEGAICLQTSAKEISPDFINVKGEVTGDIRVEITD
                     ASRQTLCNALKLQPDEDGIGATLQPA"
     gene            complement(9830..11203)
                     /gene="mdtK"
                     /locus_tag="ACHOSC_RS02460"
                     /old_locus_tag="pgaptmp_000492"
     CDS             complement(9830..11203)
                     /gene="mdtK"
                     /locus_tag="ACHOSC_RS02460"
                     /old_locus_tag="pgaptmp_000492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_025307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MATE transporter MdtK"
                     /protein_id="HGU4546493.1"
                     /translation="MQKYISEARLLLALAIPVILAQIAQTAMGFVDTVMAGGYSATDM
                     AAVAIGTSIWLPAILFGHGLLLALTPVIAQLNGSGRRERIAHQVRQGFWLAGFVSVLI
                     MLVLWNAGYIIRSMENIDPALADKAVGYLRALLWGAPGYLFFQVARNQCEGLAKTKPG
                     MVMGFIGLLVNIPVNYIFIYGHFGMPELGGVGCGVATAAVYWVMFLAMVSYIKRARSM
                     RDIRNEKGTAKPDPAVMKRLIQLGLPIALALFFEVTLFAVVALLVSPLGIVDVAGHQI
                     ALNFSSLMFVLPMSLAAAVTIRVGYRLGQGSTLDAQTAARTGLMVGVCMATLTAIFTV
                     SLREQIALLYNDNPEVVTLAAHLMLLAAVYQISDSIQVIGSGILRGYKDTRSIFYITF
                     TAYWVLGLPSGYILALTDLVVEPMGPAGFWIGFIIGLTSAAIMMMLRMRFLQRLPSAI
                     ILQRASR"
     gene            11418..12059
                     /gene="ribE"
                     /locus_tag="ACHOSC_RS02465"
                     /old_locus_tag="pgaptmp_000493"
     CDS             11418..12059
                     /gene="ribE"
                     /locus_tag="ACHOSC_RS02465"
                     /old_locus_tag="pgaptmp_000493"
                     /EC_number="2.5.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004746 - riboflavin synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="riboflavin synthase"
                     /protein_id="HGU4546494.1"
                     /translation="MFTGIVQGTAKLVSIDEKPNFRTHVVELPDHMLDGLETGASVAH
                     NGCCLTVTEINGNHVSFDLMKETLRITNLGDLKVGDWVNVERAAKFSDEIGGHLMSGH
                     IMTTAEVAKILTSENNRQIWFKVQDSQLMKYILYKGFIGIDGISLTVGEVTPTRFCVH
                     LIPETLERTTLGKKKLGARVNIEIDPQTQAVVDTVERVLAARENAMNQPGTEA"
     gene            complement(12099..13247)
                     /gene="cfa"
                     /locus_tag="ACHOSC_RS02470"
                     /old_locus_tag="pgaptmp_000494"
     CDS             complement(12099..13247)
                     /gene="cfa"
                     /locus_tag="ACHOSC_RS02470"
                     /old_locus_tag="pgaptmp_000494"
                     /EC_number="2.1.1.79"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclopropane fatty acyl phospholipid synthase"
                     /protein_id="HGU4546495.1"
                     /translation="MSSSCIEEVSVPDDNWYRIANELLSRAGIAINGSAPADIRVKNP
                     DFFKRVLQEGSLGLGESYMDGWWECDRLDMFFSKVLRAGLENQLPHHFKDTLRIAGAR
                     LFNLQSKKRAWIVGKEHYDLGNDLFSRMLDPFMQYSCAYWKDADNLESAQQAKLKMIC
                     EKLQLKPGMRVLDIGCGWGGLAHYMASNYDVSVVGVTISAEQQKMAQERCEGLDVTIL
                     LQDYRDLNDQFDRIVSVGMFEHVGPKNYDTYFAVVDRNLKPEGIFLLHTIGSKKTDLN
                     VDPWINKYIFPNGCLPSVRQIAQSSEPHFVMEDWHNFGADYDTTLMAWYERFLAAWPE
                     IADNYSERFKRMFTYYLNACAGAFRARDIQLWQVVFSRGVENGLRVAR"
     gene            complement(13538..14749)
                     /gene="punC"
                     /locus_tag="ACHOSC_RS02475"
                     /old_locus_tag="pgaptmp_000495"
     CDS             complement(13538..14749)
                     /gene="punC"
                     /locus_tag="ACHOSC_RS02475"
                     /old_locus_tag="pgaptmp_000495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042910 - xenobiotic transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:1990961 - xenobiotic detoxification by
                     transmembrane export across the plasma membrane [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="purine nucleoside transporter PunC"
                     /protein_id="HGU4546496.1"
                     /translation="MQPGKRFLVWLAGLSVLGFLATDMYLPAFAAIQADLQTPASAVS
                     ASLSLFLAGFAAAQLLWGPLSDRYGRKPVLLIGLTIFALGSLGMLWVENAATLLVLRF
                     VQAVGVCAAAVIWQALVTDYYPSQKVNRIFATIMPLVGLSPALAPLLGSWLLVHFSWQ
                     AIFATLFAITVVLILPIFWLKPTTKARNNSQDGLTFTDLLRSKTYRGNVLIYAACSAS
                     FFAWLTGSPFILSEMGYSPAVIGLSYVPQTIAFLIGGYGCRAALQKWQGKQLLPWLLV
                     LFAVSVIATWAAGFISHVSLVEILIPFCVMAIANGAIYPIVVAQALRPFPHATGRAAA
                     LQNTLQLGLCFLASLVVSWLISISTPLLTTTSVMLSTVVLVALGYMMQRCEEVGCQNH
                     GNAEVAHSESH"
     gene            14862..15794
                     /gene="punR"
                     /locus_tag="ACHOSC_RS02480"
                     /old_locus_tag="pgaptmp_000496"
     CDS             14862..15794
                     /gene="punR"
                     /locus_tag="ACHOSC_RS02480"
                     /old_locus_tag="pgaptmp_000496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional activator PunR"
                     /protein_id="HGU4546497.1"
                     /translation="MWSEYSLEVVDAVARNGSFSAAAQELHRVPSAVSYTVRQLEEWL
                     AVPLFERRHRDVELTAAGAWFLKEGRSVVKKMQITRQQCQQIANGWRGQLAIAVDNIV
                     RPERTRQMIVDFYRHFDDVELLVFQEVFNGVWDALSDGRVELAIGATRAIPVGGRYAF
                     RDMGMLSWSCVVASHHPLALMDGPFSDDTLRNWPSLVREDTSRTLPKRITWLLDNQKR
                     VVVPDWESSATCISAGLCIGMVPTHFAKPWLNEGKWVALELENPFPDSACCLTWQQND
                     MSPALTWLLEYLGDSETLNKEWLREPEETPATGD"
     gene            complement(15791..16816)
                     /gene="purR"
                     /locus_tag="ACHOSC_RS02485"
                     /old_locus_tag="pgaptmp_000497"
     CDS             complement(15791..16816)
                     /gene="purR"
                     /locus_tag="ACHOSC_RS02485"
                     /old_locus_tag="pgaptmp_000497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005127190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional repressor PurR"
                     /protein_id="HGU4546498.1"
                     /translation="MATIKDVAKRANVSTTTVSHVINKTRFVAEETRNAVWAAIKELH
                     YSPSAVARSLKVNHTKSIGLLATSSEAAYFAEIIEAVEKNCFQKGYTLILGNAWNNLE
                     KQRAYLSMMAQKRVDGLLVMCSEYPEPLLAMLEEYRHIPMVVMDWGEAKADFTDAVID
                     NAFEGGYMAGRYLIERGHREIGVIPGPLERNTGAGRLAGFMKAMEEAMIKVPESWIVQ
                     GDFEPESGYRAMQQILSQPHRPTAVFCGGDIMAMGALCAADEMGLRVPQDVSLIGYDN
                     VRNARYFTPALTTIHQPKDSLGETAFNMLLDRIVNKREEPQSIEVHPRLIERRSVADG
                     PFRDYRR"
     gene            17115..17204
                     /gene="cydH"
                     /locus_tag="ACHOSC_RS02490"
                     /old_locus_tag="pgaptmp_000498"
                     /gene_synonym="ynhF"
     CDS             17115..17204
                     /gene="cydH"
                     /locus_tag="ACHOSC_RS02490"
                     /old_locus_tag="pgaptmp_000498"
                     /gene_synonym="ynhF"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome bd-I accessory subunit CydH"
                     /protein_id="HGU4546499.1"
                     /translation="MSTDLKFSLVTTIIVLGLIVAVGLTAALH"
     gene            17370..18539
                     /locus_tag="ACHOSC_RS02495"
                     /old_locus_tag="pgaptmp_000499"
     CDS             17370..18539
                     /locus_tag="ACHOSC_RS02495"
                     /old_locus_tag="pgaptmp_000499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HGU4546500.1"
                     /translation="MKINYPLLALAIGAFGIGTTEFSPMGLLPVIARGVDVSIPAAGM
                     LISAYAVGVMVGAPLMTLLLSHRARRSALIFLMAIFTLGNVLSAIAPDYMTLMLSRIL
                     TSLNHGAFFGLGSVVAASVVPKHKQASAVATMFMGLTLANIGGVPAATWLGETIGWRM
                     SFLATAGLGVISMVSLFFSLPKGGAGARPEVKKELAVLMRPQVLSALLTTVLGAGAMF
                     TLYTYISPVLQSITHATPVFVTAMLVLIGVGFSIGNYLGGKLADRSVNGTLKGFLLLL
                     MVIMLAIPFLARNEFGAAISMVVWGAATFAVVPPLQMRVMRVASEAPGLSSSVNIGAF
                     NLGNALGAAAGGAVISAGLGYSFVPVMGAIVAGLALLLVFMSARKQPETVCVANS"
     gene            complement(18685..19266)
                     /gene="sodB"
                     /locus_tag="ACHOSC_RS02500"
                     /old_locus_tag="pgaptmp_000500"
     CDS             complement(18685..19266)
                     /gene="sodB"
                     /locus_tag="ACHOSC_RS02500"
                     /old_locus_tag="pgaptmp_000500"
                     /EC_number="1.15.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004784 - superoxide dismutase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006801 - superoxide metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase [Fe]"
                     /protein_id="HGU4546501.1"
                     /translation="MSFELPALPYAKDALAPHISAETIEYHYGKHHQTYVTNLNNLIK
                     GTAFEGKSLEEIIRSSEGGVFNNAAQVWNHTFYWNCLAPNAGGEPTGKVAEAIAASFG
                     SFADFKAQFTDAAIKNFGSGWTWLVKNSDGKLAIVSTSNAGTPLTTDATPLLTVDVWE
                     HAYYIDYRNARPGYLEHFWALVNWEFVAKNLAA"
     gene            complement(19394..20221)
                     /gene="mepH"
                     /locus_tag="ACHOSC_RS02505"
                     /old_locus_tag="pgaptmp_000501"
     CDS             complement(19394..20221)
                     /gene="mepH"
                     /locus_tag="ACHOSC_RS02505"
                     /old_locus_tag="pgaptmp_000501"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-endopeptidase MepH"
                     /protein_id="HGU4546502.1"
                     /translation="MARINRISITLCALLFTTLPLTPMAHASKQARESSATTHITKKA
                     DKKKSTATTKKTQKASQKTAKKAASKSTTKSKTASSVKKSSITASKNAKTRSKHAVNK
                     TASASFTEKCTKRKGYKSHCVKVKNAASGTLADAHKAKVQKATKVAMNKLMQQIGKPY
                     RWGGSSPRTGFDCSGLVYYAYKDLVKIRIPRTANEMYHLRDAAPIERSELKNGDLVFF
                     RTQGRGTADHVGVYVGNGKFIQSPRTGQEIQITSLSEDYWQRHYVGARRVMTPKTLR"
     gene            20556..20903
                     /gene="grxD"
                     /locus_tag="ACHOSC_RS02510"
                     /old_locus_tag="pgaptmp_000502"
     CDS             20556..20903
                     /gene="grxD"
                     /locus_tag="ACHOSC_RS02510"
                     /old_locus_tag="pgaptmp_000502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="monothiol glutaredoxin 4"
                     /protein_id="HGU4546503.1"
                     /translation="MSTTIEKIQRQIAENPILLYMKGSPKLPSCGFSAQAVQALAACG
                     ERFAYVDILQNPDIRAELPKYANWPTFPQLWVDGELVGGCDIVIEMYQRGELQQLIKE
                     TAAKYKSEEPDAE"
     gene            complement(20954..25570)
                     /locus_tag="ACHOSC_RS02515"
                     /old_locus_tag="pgaptmp_000503"
     CDS             complement(20954..25570)
                     /locus_tag="ACHOSC_RS02515"
                     /old_locus_tag="pgaptmp_000503"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016818 - hydrolase activity, acting on
                     acid anhydrides, in phosphorus-containing anhydrides
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent helicase"
                     /protein_id="HGU4546504.1"
                     /translation="MADNPDPSSLLPDVFSPATRDWFLRAFKQPTAVQSQTWHVAARS
                     EHALVIAPTGSGKTLAAFLYALDRLFREGGEDTREAHKRKTSRILYISPIKALGTDVQ
                     RNLQLPLKGIADERRRRGETEVNLRVGIRTGDTPAQECSKLTRNPPDILITTPESLYL
                     MLTSRARETLRGVETVIIDEVHAVAGSKRGAHLALSLERLDALLHTSAQRIGLSATVR
                     SASDVAAFLGGDRPVTVVNPPAMRHPQIRIVVPVANMDDVSSVASGTGEDSHAGREGS
                     IWPYIETGILDEVLRHRSTIVFTNSRGLAEKLTARLNELYAARLQRSPSIAVDTAHFE
                     STSGATSNHVQSSDVFIARSHHGSVSKEQRAITEQALKSGELRCVVATSSLELGIDMG
                     AVDLVIQVATPLSVASGLQRIGRAGHQVGGVSKGLFFPRTRRDLVDSAVIVECMFAGR
                     LENLTPPHNPLDVLAQQTVAAAAMDALQVDEWYSRVRRAAPWKDLPRRVFDATLDMLS
                     GRYPSGDFSAFRPKLVWNRETGILTARPGAQLLAVTSGGTIPDRGMYSVLLPEGEEQA
                     GSRRVGELDEEMVYESRVSDIITLGATSWRIQQITRDQVIVTPAPGRSARLPFWRGEG
                     NGRPAELGEMIGDFLHLLADGAFFSGTIPPWLAEENTNANIQGLIDEQRNATGIVPGS
                     RHLVLERCRDEIGDWRIILHSPYGRRVHEPWALAIAGRIHALWGADASVVASDDGIVA
                     RIPDTDGKLPDAAIFLFEPEKLLQIVREAVGSSALFAARFRECTARALLMPGRTPGHR
                     TPLWQQRLRASQLLEIAQGYPDFPVILETLRECLQDVYDLPALERLMRRLNGGEIQIS
                     DVTTTTPSPFATSLLFGYVAEFMYQSDAPLAERRASVLSLDSELLRNLLGQVDPGELL
                     DPQVIRQVEEELQRLAPGRRAKGEEGLFDLLRELGPMTVEDLAQRHTGSSEEVASYLE
                     NLLAVKRIFPAMISGQERLACMDDAARLRDALGVRLPESLPEIYLHRVSYPLRDLFLR
                     YLRAHALVTAEQLAHEFSLGIAIVEEQLQQLREQGLVMNLQQDIWVSDEVFRRLRLRS
                     LQAAREATRPVAATTYARLLLERQGVLPATDGSPALFASTSPGVYEGVDGVMRVIEQL
                     AGVGLPASLWESQILPARVRDYSPEMLDELLATGAVIWSGQKKLGEDDGLVALHLQEY
                     AAESFTPAEADQANRSALQQAIIAVLADGGAWFAQQISQRIRDKIGESVDLSALQEAL
                     WALVWQGVITSDIWAPLRALTRSSSNARTSTRRSHRARRGRPVYAQPVSPLVSYNTPN
                     LAGRWSLLQVEPLNDTERMLALAENMLDRYGIISRQAVIAENIPDGFPSMQTLCRSME
                     DSGRIMRGRFVEGLGGAQFAERLTIDRLRDLATQATQTRHYTPVALSANDPANVWGNL
                     LPWPAHPATLVPTRRAGALVVVSGGKLLLYLAQGGKKMLVWQEKEELLAPEVFHALTT
                     ALRREPRLRFTLTEVNDLPVRQTPMFTLLREAGFSSSPQGLDWG"
     gene            complement(25663..26310)
                     /gene="rnt"
                     /locus_tag="ACHOSC_RS02520"
                     /old_locus_tag="pgaptmp_000504"
     CDS             complement(25663..26310)
                     /gene="rnt"
                     /locus_tag="ACHOSC_RS02520"
                     /old_locus_tag="pgaptmp_000504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016896 - RNA exonuclease activity,
                     producing 5'-phosphomonoesters [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA];
                     GO_process: GO:0042780 - tRNA 3'-end processing [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease T"
                     /protein_id="HGU4546505.1"
                     /translation="MSDNAQLTGLCDRFRGFYPVVIDVETAGFNAKTDALLEIAAITL
                     KMDEQGWLMPDTTLHFHVEPFVGANLQPEALAFNGIDPNDPDRGAVSEYEALHEIFKV
                     VRKGIKASGCNRAIMVAHNANFDHSFMMAAAERASLKRNPFHPFATFDTAALAGLALG
                     QTVLSKACQTAGMDFDSTQAHSALYDTERTAVLFCEIVNRWKRLGGWPLPAAEEV"
     gene            complement(26413..26820)
                     /gene="gloA"
                     /locus_tag="ACHOSC_RS02525"
                     /old_locus_tag="pgaptmp_000505"
     CDS             complement(26413..26820)
                     /gene="gloA"
                     /locus_tag="ACHOSC_RS02525"
                     /old_locus_tag="pgaptmp_000505"
                     /EC_number="4.4.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008500591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactoylglutathione lyase"
                     /protein_id="HGU4546506.1"
                     /translation="MRLLHTMLRVGDLQRSIDFYTKVLGMKLLRTSENPEYKYSLAFV
                     GYGPETEEAVIELTYNWGVDKYELGTAYGHIALSVDNAAEACEKIRQNGGNVTREAGP
                     VKGGTTVIAFVEDPDGYKIELIEEKDAGRGLGN"
     gene            complement(26901..27998)
                     /gene="nemA"
                     /locus_tag="ACHOSC_RS02530"
                     /old_locus_tag="pgaptmp_000506"
     CDS             complement(26901..27998)
                     /gene="nemA"
                     /locus_tag="ACHOSC_RS02530"
                     /old_locus_tag="pgaptmp_000506"
                     /EC_number="1.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-ethylmaleimide reductase"
                     /protein_id="HGU4546507.1"
                     /translation="MSSEKLYSPLKVGAITAANRIFMAPLTRLRSIEPGDIPTPLMAE
                     YYRQRASAGLIISEATQISAQAKGYAGAPGIHSPEQIAAWKKITAGVHAENGHMAVQL
                     WHTGRISHASLQPGGQAPVAPSALSAGTRTSLRDENGQAIRVETSMPRALELEEIPGI
                     VNDFRQAIANAREAGFDLVELHSAHGYLLHQFLSPSSNHRTDQYGGSVENRARLVLEV
                     VDAGIEEWGADRIGIRVSPIGTFQNTDNGANEEADALYLIEQLGKRGIAYLHMSEPDW
                     AGGEPYTDAFREKVRARFHGPIIGAGAYTVEKAETLIGKGLIDAVAFGRDWIANPDLV
                     ARLQRKAELNPQRAESFYGGGAEGYTDYPTL"
     gene            complement(28035..28634)
                     /gene="nemR"
                     /locus_tag="ACHOSC_RS02535"
                     /old_locus_tag="pgaptmp_000507"
     CDS             complement(28035..28634)
                     /gene="nemR"
                     /locus_tag="ACHOSC_RS02535"
                     /old_locus_tag="pgaptmp_000507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416166.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator NemR"
                     /protein_id="HGU4546508.1"
                     /translation="MNKHTEHDTREHLLATGEQLCLQRGFTGMGLSELLKTAEVPKGS
                     FYHYFRSKEAFGVAMLERHYAAYHQRLTELLQSGEGNYRDRILAYYQQTLNQFCQHGT
                     ISGCLTVKLSAEVCDLSEDMRSAMDKGARGVIALLSQALENGRENHCLTFCGEPLQQA
                     QVLYALWLGANLQAKISRSFEPLENALAHVKNIIATPAV"
     gene            28737..28976
                     /locus_tag="ACHOSC_RS02540"
                     /old_locus_tag="pgaptmp_000508"
     CDS             28737..28976
                     /locus_tag="ACHOSC_RS02540"
                     /old_locus_tag="pgaptmp_000508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416165.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1289 domain-containing protein"
                     /protein_id="HGU4546509.1"
                     /translation="MAEQLEFFPVQSPCRGICQSDERGFCRGCFRSRDERFNWNKMSD
                     GEKQEVLRLCRQRLMRKLRANKPASSDEPEQPSLF"
     gene            29025..29921
                     /locus_tag="ACHOSC_RS02545"
                     /old_locus_tag="pgaptmp_000509"
     CDS             29025..29921
                     /locus_tag="ACHOSC_RS02545"
                     /old_locus_tag="pgaptmp_000509"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_025305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase family oxidoreductase"
                     /protein_id="HGU4546510.1"
                     /translation="MVQRITIAPQGPEFSRFVMGYWRLMDWNMSARQLVSFIEEHLDL
                     GVTTVDHADIYGGYQCEAAFGEALKLAPHLRERMEIVSKCGIATTAREENVIGHYITD
                     RDHIIKSAEQSLINLATDHLDLLLIHRPDPLMDADEVADAFKHLHQSGKVRHFGVSNF
                     TPAQFALLQSRLPFTLATNQVEISPVHQPLLLDGTLDQLQQLRVRPMAWSCLGGGRLF
                     NDDYFQQLRDELAVVAEELNAGSIEQVVYAWVLRLPSQPLPIIGSGKIERVRAAVEAE
                     TLKMTRQQWFRIRKAALGYDVP"
     gene            30002..30523
                     /gene="sodC"
                     /locus_tag="ACHOSC_RS02550"
                     /old_locus_tag="pgaptmp_000510"
     CDS             30002..30523
                     /gene="sodC"
                     /locus_tag="ACHOSC_RS02550"
                     /old_locus_tag="pgaptmp_000510"
                     /EC_number="1.15.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004784 - superoxide dismutase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase [Cu-Zn] SodC"
                     /protein_id="HGU4546511.1"
                     /translation="MKRFSLAILALVVATGAQAASEKVEMNLVTSQGVGQSIGSVTIT
                     ETDKGLEFSPDLKALPPGEHGFHIHAKGSCQPATKDGKASAAESAGGHLDPQNTGKHE
                     GPEGAGHLGDLPALVVNNDGKATDAVIAPRLKSLDEIKDKALMVHVGGDNMSDQPKPL
                     GGGGERYACGVIK"
     gene            complement(30524..32536)
                     /locus_tag="ACHOSC_RS02555"
                     /old_locus_tag="pgaptmp_000511"
     CDS             complement(30524..32536)
                     /locus_tag="ACHOSC_RS02555"
                     /old_locus_tag="pgaptmp_000511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HGU4546512.1"
                     /translation="MNASSWSLRNLPWFKATLAQWRYALRNTIAMCLALTVAYYLNLD
                     EPYWAMTSAAVVSFPTVGGVISKSLGRIAGSLLGAIAALLLAGHTLNEPWFFLLSMSA
                     WLGFCTWACAHFTNNVAYAFQLAGYTAAIIAFPMVNITEASQLWDIAQARVCEVIVGI
                     LCGGMMMMILPSSSDATALLTALKNMHARLLEHASLLWQPETNDAIRAAHEGVIGQIL
                     TMNLLRIQAFWSHYRFRQQNARLNALLHQQLRMTSVISSLRRMLLNWPSPPGATREIL
                     EQLLTALASSQTDAYTVARIIAPLRPTNVADYRHVAFWQRLRYFCRLYLQSSQELHRL
                     QSGVDDHARLARTSALARHTDNAEAMWSGLRTFCTLMMIGAWSIASQWDAGANALTLA
                     AISCVLYSAVAAPFKSLSLLMRTLVLLSLFSFVVKFGLMVQISDLWQFLLFLFPLLAT
                     MQLLKLQMPKFAALWGQLIVFMGSFIAVTNPPVYDFADFLNDNLAKIVGVALAWLAFA
                     ILRPGSDARKSRRHIRALRRDFVDQLSRHPTLSESEFESLTYHHVSQLSNSQDALARR
                     WLLRWGVVLLNCSHVVWQLRDWESRSDPLSRVRDNCISLLRGVMSERGVQQKSLAATL
                     EELQRICDSLARHHQPAARELAAIVWRLYCSLSQLEQAPPQGTLAS"
     gene            complement(32536..33393)
                     /locus_tag="ACHOSC_RS02560"
                     /old_locus_tag="pgaptmp_000512"
     CDS             complement(32536..33393)
                     /locus_tag="ACHOSC_RS02560"
                     /old_locus_tag="pgaptmp_000512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310380.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HGU4546513.1"
                     /translation="MSIKTIKYFSTIIVAIVAVLAGWWLWNYYMQSPWTRDGKIRAEK
                     VSITPQVSGRIVELNIKDNQLVNAGDLLLTIDKTPFQIAELNAQAQLAKAQSDLAKAN
                     NEANRRRHLSQNFISAEELDTANLNVKAMQASVNAAQATLKQTQWQLAQTEIRAPVSG
                     WVTNLTTRIGDYADTGKPLFALVDSHSFYVIGYFEETKLRHIREGAPAQITLYSDNKT
                     LRGHVSSIGRAIYDQSVESDSSLIPDVKPNVPWVRLAQRVPVRFALDKVPGDVTLVSG
                     TTCSIAVGQ"
     gene            complement(33396..33632)
                     /locus_tag="ACHOSC_RS02565"
                     /old_locus_tag="pgaptmp_000513"
     CDS             complement(33396..33632)
                     /locus_tag="ACHOSC_RS02565"
                     /old_locus_tag="pgaptmp_000513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1656 domain-containing protein"
                     /protein_id="HGU4546514.1"
                     /translation="MKFMLNATGLPLQDLVFGASVYFPPFFKAFAFGFVIWLVVHRLL
                     RGWIYAGDIWHPLLMDLSLFAICVCLALAILIAW"
     gene            33833..34267
                     /gene="slyA"
                     /locus_tag="ACHOSC_RS02570"
                     /old_locus_tag="pgaptmp_000514"
     CDS             33833..34267
                     /gene="slyA"
                     /locus_tag="ACHOSC_RS02570"
                     /old_locus_tag="pgaptmp_000514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416159.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator SlyA"
                     /protein_id="HGU4546515.1"
                     /translation="MESPLGSDLARLVRIWRALIDHRLKPLELTQTHWVTLHNIHQLP
                     PDQSQIQLAKAIGIEQPSLVRTLDQLEEKGLISRQTCASDRRAKRIKLTEKAEPLISE
                     MEAVINKTRAEILHGISAEELEQLITLIAKLEHNIIELQAKG"
     gene            complement(34314..34781)
                     /gene="slyB"
                     /locus_tag="ACHOSC_RS02575"
                     /old_locus_tag="pgaptmp_000515"
     CDS             complement(34314..34781)
                     /gene="slyB"
                     /locus_tag="ACHOSC_RS02575"
                     /old_locus_tag="pgaptmp_000515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipoprotein SlyB"
                     /protein_id="HGU4546516.1"
                     /translation="MIKRVLVVSMVGLSLVGCVNNDTLSGDVYTASEAKQVQNVSYGT
                     IVNVRPVQIQGGDDSNVIGAIGGAVLGGFLGNTVGGGTGRSLATAAGAVAGGVAGQGV
                     QSAMNKTQGVELEIRKDDGNTIMVVQKQGNTRFSPGQRVVLASNGSQVTVSPR"
     gene            35055..36164
                     /gene="anmK"
                     /locus_tag="ACHOSC_RS02580"
                     /old_locus_tag="pgaptmp_000516"
     CDS             35055..36164
                     /gene="anmK"
                     /locus_tag="ACHOSC_RS02580"
                     /old_locus_tag="pgaptmp_000516"
                     /EC_number="2.7.1.170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anhydro-N-acetylmuramic acid kinase"
                     /protein_id="HGU4546517.1"
                     /translation="MKSGRFIGVMSGTSLDGVDVVLATIDEHRVAQLASLSWPIPVSL
                     KQAVLDICQGQQLTLSQFGQLDTQLGRLFADAVNALLKEQNLQARDIVAIGCHGQTVW
                     HEPTGVEPHTLQIGDNNQIVARTGITVVGDFRRRDIALGGQGAPLVPAFHHALLAHPT
                     ERRMVLNIGGIANLSLLIPGQPVGGYDTGPGNMLMDAWIWRQAGKPYDKDAEWARAGK
                     VILPLLQNMLSDPYFSQPAPKSTGREYFNYGWLERHLRHFPGVDPRDVQATLAELTAV
                     TISEQVLLSGGCERLMVCGGGSRNPLLMARLAALLPGTEVTTTDAVGISGDDMEALAF
                     AWLAWRTLAGLPGNLPSVTGASQETVLGAIFPANP"
     gene            36262..36591
                     /gene="mliC"
                     /locus_tag="ACHOSC_RS02585"
                     /old_locus_tag="pgaptmp_000517"
     CDS             36262..36591
                     /gene="mliC"
                     /locus_tag="ACHOSC_RS02585"
                     /old_locus_tag="pgaptmp_000517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416156.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C-type lysozyme inhibitor"
                     /protein_id="HGU4546518.1"
                     /translation="MSMKKLLLICLPVLLTGCSAFNQLVEHMQTATLEYQCDEKPLTV
                     KLNNPRQEVSFVYDNQLLHLKQGISASGARYTDGIYVFWSKGDEATVYKRDRIVLNNC
                     QLQNPQR"
     gene            36650..37306
                     /gene="pdxH"
                     /locus_tag="ACHOSC_RS02590"
                     /old_locus_tag="pgaptmp_000518"
     CDS             36650..37306
                     /gene="pdxH"
                     /locus_tag="ACHOSC_RS02590"
                     /old_locus_tag="pgaptmp_000518"
                     /EC_number="1.4.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005127173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004733 - pyridoxamine phosphate oxidase
                     activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase"
                     /protein_id="HGU4546519.1"
                     /translation="MSDNDELQQIAHLRREYTKGGLRRRDLPADPLTLFERWLSQACE
                     AKLADPTAMVVATVDEHAQPYQRIVLLKHYDEKGMVFYTNLGSRKAHQIENNPRVSLL
                     FPWHTLERQVMVIGKAERLSTLEVMKYFHSRPRDSQIGAWVSKQSSRISARGILESKF
                     LELKQKFQQGEVPLPSFWGGFRVSLEQIEFWQGGEHRLHDRFLYQRENDAWKIDRLAP
                     "
     gene            37435..38709
                     /gene="tyrS"
                     /locus_tag="ACHOSC_RS02595"
                     /old_locus_tag="pgaptmp_000519"
     CDS             37435..38709
                     /gene="tyrS"
                     /locus_tag="ACHOSC_RS02595"
                     /old_locus_tag="pgaptmp_000519"
                     /EC_number="6.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004831 - tyrosine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006437 - tyrosyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine--tRNA ligase"
                     /protein_id="HGU4546520.1"
                     /translation="MASSNLIKQLQERGLVAQVTDEEALAERLAQGPIALYCGFDPTA
                     DSLHLGHLVPLLCLKRFQQAGHKPVALVGGATGLIGDPSFKAAERKLNTEETVQEWVD
                     KIRKQVAPFLDFDCGENSAIAANNYDWFGNMNVLTFLRDIGKHFSVNQMINKEAVKQR
                     LNREDQGISFTEFSYNLLQGYDFACLNKQYGVVLQIGGSDQWGNITSGIDLTRRLHQN
                     QVFGLTVPLITKADGTKFGKTEGGAVWLDPKKTSPYKFYQFWINTADADVYRFLKFFT
                     FMSIEEINALEEEDKNSGKAPRAQYVLAEQVTRLVHGEEGLQAAKRITECLFSGSLSA
                     LSEADFEQLAQDGVPMVEMEKGADLMQALVDSELQPSRGQARKTIASNAITINGEKQS
                     DPEYFFKEEDRLFGRFTLLRRGKKNYCLICWK"
     gene            38768..39631
                     /gene="pdxY"
                     /locus_tag="ACHOSC_RS02600"
                     /old_locus_tag="pgaptmp_000520"
     CDS             38768..39631
                     /gene="pdxY"
                     /locus_tag="ACHOSC_RS02600"
                     /old_locus_tag="pgaptmp_000520"
                     /EC_number="2.7.1.35"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008478 - pyridoxal kinase activity
                     [Evidence IEA];
                     GO_process: GO:0009443 - pyridoxal 5'-phosphate salvage
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal kinase"
                     /protein_id="HGU4546521.1"
                     /translation="MMKNILAIQSHVVYGHAGNSAAEFPMRRLGANVWPLNTVQFSNH
                     TQYGKWTGCVMPPSHLTEIVQGIAAIDKLHTCDAVLSGYLGSAEQGEHILGIVRQVKA
                     ANPQAKYFCDPVMGHPEKGCIVAPGVAEFHVRHGLPASDIIAPNLVELEILCEHAVNN
                     VEEAVLAARELIAQGPQIVLVKHLARAGYSRDRFEMLLVTADEAWHISRPLVDFGMRQ
                     PVGVGDVTSGLLLVKLLQGATLQEALEHVTAAVYEIMVTTKAMQEYELQVVAAQDRIA
                     KPEHYFSATKL"
     gene            complement(39675..40280)
                     /gene="gstA"
                     /locus_tag="ACHOSC_RS02605"
                     /old_locus_tag="pgaptmp_000521"
     CDS             complement(39675..40280)
                     /gene="gstA"
                     /locus_tag="ACHOSC_RS02605"
                     /old_locus_tag="pgaptmp_000521"
                     /EC_number="2.5.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione transferase GstA"
                     /protein_id="HGU4546522.1"
                     /translation="MKLFYKPGACSLASHITLRESGKDFTLVSVDLMKKRLENGDDYF
                     AVNPKGQVPALLLDDGTLLTEGVAIMQYLADSVPDRQLLAPVNSISRYKTIEWLNYIA
                     TELHKGFTPLFRPDTPEEYKPTVRAQLEKKLQYVNEALKDEHWICGQRFTIADAYLFT
                     VLRWAYAVKLNLEGLEHIAAFMQRMAERPEVQDALSAEGLK"
     gene            complement(40386..41888)
                     /gene="dtpA"
                     /locus_tag="ACHOSC_RS02610"
                     /old_locus_tag="pgaptmp_000522"
     CDS             complement(40386..41888)
                     /gene="dtpA"
                     /locus_tag="ACHOSC_RS02610"
                     /old_locus_tag="pgaptmp_000522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:1904680 - peptide transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015833 - peptide transport [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide/tripeptide permease DtpA"
                     /protein_id="HGU4546523.1"
                     /translation="MSTANQKPTESVSLNAFKQPKAFYLIFSIELWERFGYYGLQGIM
                     AVYLVKQLGMSEADSITLFSSFSALVYGLVAIGGWLGDKVLGTKRVIMLGAIVLAIGY
                     ALVAWSGHDAGIVYMGMAAIAVGNGLFKANPSSLLSTCYEKNDPRLDGAFTMYYMSVN
                     IGSFFSMIATPWLAAKYGWSVAFALSVVGLLITIVNFAFCQRWVKQYGSKPDFEPINY
                     RNLLLTIIGVVALIAIATWLLHNQEVARMALGVVAFGIVVIFGKEAFAMKGAARRKMI
                     VAFILMLEAIIFFVLYSQMPTSLNFFAIRNVEHSILGLAVEPEQYQALNPFWIIIGSP
                     ILAAIYNKMGDTLPMPTKFAIGMVMCSGAFLILPLGAKFASDAGIVSVSWLVASYGLQ
                     SIGELMISGLGLAMVAQLVPQRLMGFIMGSWFLTTAGANLIGGYVAGMMAVPDNVTDP
                     LMSLEVYGRVFLQIGVATAIIAVLMLLTAPKLHRMTQDDAADKAAKAAVA"
BASE COUNT        10641 a        10405 c        11044 g        10128 t
ORIGIN      
        1 cacattattt tttctcctaa ccataaagga ttagtttatc ggcgttaaat cttttctcat
       61 acactatcgg gttagggaaa atatctggtg ttggggacag agtcagcagc gtattagttg
      121 aatgcttaat atcaggctgc tgaattgtca gcactgtgtt tgcgaagagg atgcgaaata
      181 aatgaacccg gttgatcgtc cactattaga tattggctta acgcgattag aattcttgcg
      241 tatatcagga aaaggccttg ctggtctaac aattgcccct gcgttactgt cacttttggg
      301 ttgcaaacag gaagatattg atagcggcac agtagggttg ataaatacac ccaaaggggt
      361 gctggtgacc cagcgagcgc gatgcacggg ttgccaccgc tgtgaaatct cttgtaccaa
      421 cttcaacgat ggctcagtag ggacattctt ctcccgtatc aaaatccacc gcaattattt
      481 ctttggcgac aacggggttg gctctggcgg cggcctgtat ggcgatctca actataccgc
      541 ggacacctgc cgtcaatgca aagagccgca atgcatgaac gtctgcccga ttggtgcgat
      601 tacctggcag cagaaagaag gctgtattac cgtcgatcat aaacgctgta ttggctgtag
      661 cgcctgtact acggcatgcc cgtggatgat ggccaccgta aataccgaaa gtaaaaaatc
      721 ctcgaaatgt gtgttatgcg gtgaatgcgc aaacgcctgc ccaacagggg cgttaaaaat
      781 tatcgagtgg aaagatatta ctgtgtgaat cttgcaaagg aaaaaatcat ggctaacggt
      841 tggacaggta atatattaag agtcaatctc acgacaggaa atattaccct cgaagattcc
      901 agtaagttta aaagttttgt cggtggcatg ggcttcggct acaaaattat gtatgacgaa
      961 gtaccgccag gcacgaaacc tttcgatgaa gcgaataaat tagtctttgc taccggccca
     1021 ttaactggat ctggtgcccc ctgtagttct cgcgtaaata tcacctcact ttctactttt
     1081 accaaaggaa atttagttgt cgatgcccat atgggtggct tttttgcagc gcaaatgaaa
     1141 ttcgctggat acgacgtcat tattatcgaa gggaaggcga aatcaccggt atggctgaag
     1201 attaaagatg acaaagttag cctggaaaaa gccgatttct tatggggaaa agggacgcgc
     1261 gcaacgacgg aagaaatttg tcgattgacc agtccggaaa cctgtgtggc ggctattggt
     1321 caggctgggg aaaaccttgt tcctctctct ggcatgttga atagccgtaa ccacagcggc
     1381 ggtgcgggaa ctggcgcaat tatgggttcg aaaaacctga aagcgattgc ggttgaaggg
     1441 acgaaagggg tcaacattgc cgatcgtcag gagatgaagc gtctcaacga ttacatgatg
     1501 actgaactta ttggtgcgaa taacaaccat gtcgtgccaa gtacgccgca atcgtgggca
     1561 gagtattcag atcccaagtc acgttggaca gcacgtaaag ggctgttttg gggcgcggct
     1621 gaaggtggtc cgattgaaac gggggaaatt ccgccaggca atcagaacac ggtcggcttt
     1681 cgtacctata aatccgtttt tgacttaggt ccggcggcag agaaatacac agtaaaaatg
     1741 agtggctgcc actcttgccc gatccgttgt atgacccaaa tgaatattcc tcgggtgaaa
     1801 gagtttggcg tgcccagcac aggtggtaac acttgtgtag ctaactttgt ccataccacc
     1861 atcttcccga acgggccgaa agattttgaa gataaagacg atggtcgtgt gattggtaac
     1921 ctggttggtc tgaatttgtt cgatgactac ggcctatggt gtaactacgg gcagctgcat
     1981 cgcgacttta cttattgtta cagcaaaggg gtgttcaagc gtgttctgcc agctgaagag
     2041 tatgcggaaa ttcactggga tcaactggaa gcgggtgacg ttaatttcat taaagatttt
     2101 tactaccgtc tggcgcatcg tgtgggtgag ctgagtcacc tggctgatgg ttcatatgcc
     2161 atcgcagaac gctggaattt gggtgaagag tactggggct acgcaaaaaa taaactctgg
     2221 tcgccgtttg gctatccggt tcaccatgcc aatgaagcgt cagcgcaggt cggttccatt
     2281 gttaactgta tgttcaaccg tgactgcatg acgcataccc atatcaattt tattggttcg
     2341 ggtttgccat tgaaactgca gcgtgaagtg gcgaaagagc tttttggttc tgaagatgcc
     2401 tacgatgaaa ccaaaaacta cacgccaatc aatgacgcaa aaattaaata tgcaaagtgg
     2461 tcgctgttgc gggtctgttt gcataacgcc gtcaccctgt gcaactgggt ctggccaatg
     2521 accgtttcgc cgctgaaaag ccgtaattac cggggcgatc tggcgcttga agccaaattc
     2581 ttcaaagcga tcaccggcga agagatgact caggaaaaat tagatttagc tgcagagcgt
     2641 atctttacgt tgcatcgtgc ctacacggta aaactgatgc aaaccaaaga tatgcgtaat
     2701 gaacacgatc ttatctgttc ctgggtattc gacaaggatc cgcagatccc ggtctttact
     2761 gaaggtactg acaaaatgga tcgtgacgat atgcatgctt cgctcacgat gttctacaaa
     2821 gaaatgggct gggacccaca gcttggttgt ccgacccgcg aaacattgca gcgtctgggg
     2881 ctggaagata ttgccgccga tctggcagca cacaatctac tgcctgtgta aggaatgggt
     2941 gagatgaacc atcgggatga attgccgtta gcgaaagtca gcgaagttga cgaggcgaag
     3001 cggcaatggt tacaggggat gcgtcacccc gttgatacgg taaccgaacc agaaccggct
     3061 gaaatcctgg cagagttcat tcgccagcat tctgcggctg ggcagctggt ggcgcgcgcc
     3121 gtgtttctct cgccacccta ttcggttgca gaagaggagc tgtcagtttt gctggaaagc
     3181 attaagcaga acggagacta cgcggatatc gcttgtatga cgggaagcca ggatgattac
     3241 tattactcaa cgcaggccat gagtgaaaac tacgcggcga tgtctttgca agttgtggag
     3301 caggacattt gtcgcgccat tgcacacgcc gttcgttttg agtgccagac atatcctcgt
     3361 ccttacaaag tcgccatgct gatgcaagcg ccatattact tccaggaagc gcagattgaa
     3421 gccgcgatcg ccgcgatgga tgttgcacct gaatatgccg atatacggca ggtggagtca
     3481 tcgacggcgg tgttgtactt gtttagcgag cgatttatga cctatggcaa ggcttatggc
     3541 ttatgcgaat ggttcgaggt agagcagttt cagaacccct gaaatttcag ggagatgtca
     3601 tggatagggt ggacagttgc agccactgca ttaggggata atcagatgtc cttcactcga
     3661 cgcaaatttg ttctggggat gggaacagta atctttttta ctggttctgc gtcttctctg
     3721 ttagcgaaca cgaggcaaga aaaggaagtt cggtatgcca tgattcatga cgagtcacgg
     3781 tgcaatggct gtaatatttg cgcccgcgca tgccgtaaaa ctaaccacgt ccccgctcag
     3841 ggaagccgtt tatcgatagc acacattcca gtcaccgata acgacaacga aacgcagtat
     3901 cacttttttc gccagtcatg tcagcactgc gaagatgctc cgtgcatcga cgtatgcccg
     3961 acaggggcgt cgtggcgtga tgagcagggg atcgtgcggg tagaaaaatc gcagtgcatc
     4021 ggctgcagtt attgcattgg cgcttgtccg taccaggttc gctaccttaa ccctgtcact
     4081 aaagtggcag ataaatgtga tttctgcgct gagtcccgac tggcaaaagg ctttccgccc
     4141 atttgcgtca gcgcctgccc ggaacacgca ttgatctttg gtcgcgaaga tagcccagaa
     4201 attcaggcgt ggttgcagca aaataaatac tatcaatatc agctacctgg tgccgggaaa
     4261 ccgcatctgt atcgccggtt cggtcaacat ttgattaaaa aggaaaatgt atgaacccgt
     4321 cgcaacatgc tgaacagttt cagagccagt tagcgaacta tgtgccacag ttcactcccg
     4381 aattttggcc ggtgtggttg atcattgccg gagtattgct ggttgggatg tggttggtgc
     4441 tggggctgca tgccttgctt cgtgctcgtg gcgtgaagaa atcagtcacc gattatggtg
     4501 agaagattta tctttactgc aaagcggtca gattatggca ctggtcgaat gcgttactct
     4561 ttgtattgtt gctggcaagt gggctgataa atcactttgc gctggtgggc gcaactgcgg
     4621 ttaaaagtct ggttgcggtg catgaagttt gcggattttt gttactcgca tgctggctcg
     4681 gctttgtgct gatcaatgcc gttgggggta atggtcacca ctatcgcatt cgtcgtcagg
     4741 ggtggctgga gcgagcggca aaacaaacgc gattttattt gtttggcatt atgcaggggg
     4801 aagaacatcc tttcccggca acaacccagt ctaaatttaa tcccttacag caggtcgcct
     4861 atgttggtgt tatgtatgga ttgctgccgt tgttactatt gacggggctg ctgtgtctct
     4921 atccgcaagc cgtgggagat gtgtttcctg gcgtaagata ctggttattg caggcgcatt
     4981 ttgctctggc atttataagc ctctttttta tcttcggtca tctttatctt tgcaccacgg
     5041 ggcgtacgcc acacgaaacc tttaaaagca tggtcgatgg ctatcaccgg cactaacgaa
     5101 tgatcattac acgagcagac ctgcgagaat ggcgtatcgg agcggtgatg tatcgctggt
     5161 tcctgcgcca tttcccacgc ggcggtagtt atgccgatat tcatcatgca ctgattgagg
     5221 aaggatatac ggactgggcg gagagtctgg ttgagtatgc gtggaaaaaa tggctggcgg
     5281 atgaaaactt cgcgcatcag gaagtttcct ctatgcaaaa actggccaca gaccccggag
     5341 agataccatt ttgcagtcag tttgcccgca gcgacgatca tgctcgcatc ggctgttgtg
     5401 aggataacgc ccgaatcgca accgctgggt atgccgcgca aattgccagt atgggttata
     5461 gcgtgcggat tggaagtgtc ggctttaaca gtcacattgg cagctccggt gaacgcgcac
     5521 gggttgctgt aaccggtaat tcttcgcgga taagcagcgc tggagatagt agtcggattg
     5581 cgaataccgg aatgcgagta cgcgtttgta cattgggtga acggtgtcat gtcgccagta
     5641 atggtgacct ggtgcaaatc gccagctttg gcgcaaacgc cagaattgcc aacagcggcg
     5701 acaatgtaca catcattgcc agcggtgaaa attcaacggt agtgagcacg ggcgtggtcg
     5761 actcaattat tctcgggcca gggggaagcg ccgcactggc atatcatgac ggcgaaagag
     5821 ttcgcttcgc cgtcgccatt gaaggtgaaa acaatattcg aaccggagtc cgctatcgtc
     5881 ttaatgaaca gcatcagttt gttgagtgct gatagcttta cctttaatca aacgaaagac
     5941 gccgacgagc acgggatgca ggcttcacga ccgcccgagc catcgcctca ccaatttctg
     6001 cacatgccgt aagtccctga acgaaaggct ggtcgtgcat caaccagggc aacgcgccga
     6061 acgctacgcg tccacgaata tcttcgggtt gctgtaacgt ataatcttcg ccaatatcag
     6121 ggatttcatc ccctgttttc tgtaattgct cgcgtagccc agggaaagga atatctttca
     6181 ctttaagcgg acgctgtccg cgggcatcaa taaaaacgtc aaacgagtag ctgttgtctt
     6241 ccgttttcag gacggtgcgc gactcattaa tttccatttt gtagtcttca ccgagggcga
     6301 gaatatgaat gattcccgct tcacgtaagg caagtaggcg acgaatagac tctgaaggga
     6361 ttgccgcata attatcgatg aaaaccctgg caaggccttt actgaaccgt ttatggtcgt
     6421 gttcattcag atgaggaaca atttcctgta cggcttcatg caggcgcaga atgacataac
     6481 gccagggaac agtatgtttt tctcgtttat tgcgttcgac ttcctggaga tttttttctg
     6541 cccagtcaaa tgggtcgcgt tgtttgcgct cggcaaacca ggcttgagca aaggaatcga
     6601 cattcaggct ctctaaggct atgcgttggc tccagtctgg atcagcaaac ttgatttcct
     6661 ctactatcaa tctaaatacc cgatccaaaa ggccctcttc acctttttga atctcagcat
     6721 ttaatgcctg atcggtgacg atgtgtaagg gctcgtaggg aatagggcaa tagaaatcgg
     6781 cttcgggtaa aatacccgtg cgcgacatca acgtaatatt tagcttttca cttgcgttat
     6841 cgcggttaaa aacgacgtgt tgtttatcat cttcaatgaa cgaaccatgc tgaatagcca
     6901 ctgccattgc cgcatccagt ccgctcaagg atgttcccat aatacccacg ttacacgcat
     6961 cgacctttgc ttccatcagg cctgaccacg gactgggaaa atacgttctg gttgcttctt
     7021 cttcatcagg ccagacgtga cccgtggcaa tcaccgctaa atcaaacgtc tcgctgggta
     7081 aatcctgatt tgtagcgagc atgacgccag catttgtaat ttgcagatcg gtaacctggc
     7141 atgattcata aacagccact gcaaattttt gctgtcgtgc ctggtctact aatcgtaaaa
     7201 attgatcgcg gaaatattcg cccagcagaa ttcgcggtaa aaactgacga tcgtgcaagg
     7261 tttctttttt taacgccata acgctggaga tggctggctt cttgcttttg tagccattcg
     7321 agatacgtac aattaatcgg cggtatttca atactggcaa tatttgccag catcattttt
     7381 gagttttcct catcactgta tggcatcccg acaccggcct cgtcagcctg ctcgaagata
     7441 gaaatagaaa gtggagtttg ttgctgtaga agcgagaata aggtgtagat ccccgtaggc
     7501 ccggcaccca caatagcaat ttttttcatt gttacttacc tgtacgcatt ctttcgaatt
     7561 tagtgttatc agataagttt ggttgagatt tttagaattt gagaaatttc agacaggtat
     7621 gaaaagggcg ctaaccgccc ttgattaaat tctgtcaggc gagttttgcc tgattgattt
     7681 gactcagtgg ctcattgaca tcgaaaactt tggcgacaac ttcctcaacg ccgagatttt
     7741 taagtcgggc agtatgtttt tccagatagg caagggcgct tttttcatca gtaaaaagat
     7801 agatcccacc ggcttcgtgg ttcttttcac tttctgtcca taccttccac agaaaaccag
     7861 gttcctgatt aatcgactca gcaagtggtt taagctgctc agccattgcg tcgccaaatg
     7921 ggccattaaa agcaaaatga agttgtaaca aagttgccat agcaattctc cttctgataa
     7981 cgcatacgaa gacagtgtaa atcttttaac gtattggata cagagaagaa acagaggaaa
     8041 aaaggagaag accagattaa gaaaatctgg tgcgtccgag tggactcgaa ccaccgaccc
     8101 ccaccatgtc aaggtggtgc tctaaccaac tgagctacgg acgcaggatg gtgcgttcaa
     8161 ttggactcga accaacgacc cccaccatgt caaggtggtg ctctaaccaa ctgagctatg
     8221 aacgcaacgt tgtaggtgac aacggggacg aatattagcg gcagagtggg aaggtggcaa
     8281 gaggcaaaac gtaattttct gcgctatttc gaccgtttgc agagctttta agcaaattgg
     8341 ctatattttg ttgatttgca agggcgattt ttattcagga tcgcatttac atctgataca
     8401 acccaataat tcgtgtatca gagtttaaac agagttgttt gccaccatct caaatctgtc
     8461 gtggataaat cctccgtagc ctttacactt aggtagcaat aaaggcacac agatgcagca
     8521 aaggaataaa tcatgggatc tgatgcgaaa aacttgatga gcgacgggaa tgtgcaaatt
     8581 gttaagaccg gcgaggtcat tggcgcgacg caacttactg aaggcgagtt gattgttgaa
     8641 gctggcggaa gagccgaaaa taccgtggtc acgggggctg gctggttgaa agtggcaacc
     8701 ggtgggatcg ccaaatgcac acagtacggt aacaatggca cgctatcggt cagcgacggt
     8761 gccattgcca cagatattgt tcagtccgag ggaggcgcaa ttagtctctc tacgctcgct
     8821 acggttaatg gccgccatcc cgaaggtgaa ttcagcgttg atcagggtta tgcctgcggt
     8881 ttgttgctgg aaaatggcgg taacctgcgt gtactggaag gacatcgcgc ggaaaaaatc
     8941 attctcgatc aagagggcgg cctgttggtc aatgggacaa cctcagctgt cgtggtagat
     9001 gaaggtggtg aattgttggt gtatccaggt ggggaagcca gcaattgtga aattaatcag
     9061 ggcggcgttt ttatgctggc cgggaaagcc agtgatacgt tgcttgctgg tggcaccatg
     9121 aataatctcg gtggtgaaga ctctgacact attgttgaga atggatccat ttatcgtctg
     9181 gggacggatg gccttcagct ctacagttcc ggtaagacgc aaaacctgtc cgtgaatgtg
     9241 ggtggtcggg ctgaagtgca tgccggtacg ctggaaaatg cggtaataca aggtggaaca
     9301 gtgatcctgt tgtcacccac cagcgcggac gaaaattttg tcgtagagga agatcgcgca
     9361 ccggttgaac tgaccgggag tgttgcatta ctggacggcg cttcaatgat tattggctat
     9421 ggtgcagatc tgcaacaatc aacgattact gtacagcagg gcggtgtgtt gattctcgac
     9481 ggcagtacgg taaaaggtga cagtgtcact tttagtgttg gtaacatcaa tctgaatggc
     9541 ggaaaactgt ggctgatcac tggtgcggca acgcatgtgc aactgaaagt gaaacgcctg
     9601 cgcggagagg gagcgatttg cctgcaaacc agtgcgaaag aaatctcacc tgacttcatc
     9661 aatgtgaaag gggaagttac cggggatata cgcgttgaga taacagatgc cagtcggcaa
     9721 actctgtgta acgcactgaa actacagcca gacgaagacg ggattggcgc aacgctccag
     9781 cctgcgtaaa aacacaggcg caacccgtga aggttgcgcc ttgtctttat tagcgggacg
     9841 ctcgttgcag aatgatggct gacggcagac gttgcaggaa ccgcatacgc agcatcatca
     9901 taatggctgc cgacgtcagg ccaataataa agcctatcca gaagcctgct ggccccatag
     9961 gttcaacgac cagatcggtc agtgccagaa tatagccgct tggcaagccc agcacccagt
    10021 aagccgtaaa ggtaatatag aaaatggaac gcgtatcttt ataaccacgc aaaatcccac
    10081 tgccaatcac ctggatcgag tcagaaatct gatataccgc cgccagcaac atcaaatgcg
    10141 cagccagcgt tacaacctcg ggattgtcgt tgtacaacag ggcgatttgc tcccgcagtg
    10201 aaaccgtgaa tatggccgtc agggttgcca tacagacacc caccataagc ccggtccgcg
    10261 cagcggtttg cgcatccagc gttgagccct gacccagacg ataacctacg cggatagtta
    10321 ccgctgccgc cagcgacatg ggaagcacga acattagtga actaaagttc agggcaatct
    10381 ggtgtcctgc gacatcaaca ataccgagcg gagacactaa cagagccacg acagcaaaca
    10441 gtgtcacttc aaagaacagt gccagcgcaa tcggcaaacc gagttgaatc agtcgtttca
    10501 taaccgcagg atcgggtttc gcggtgcctt tttcgttacg aatatcgcgc atggagcggg
    10561 cgcgtttaat gtaagaaacc atggcaagga acataaccca gtacaccgcc gcagtagcca
    10621 cgccacaacc aacgccaccg agctcaggca taccgaaatg accataaata aagatatagt
    10681 tcaccggaat gttcaccagc aggccgataa agcccattac catacccggc ttggtttttg
    10741 ccagaccttc acactggtta cgggcaacct ggaagaacag atagcccggc gcgccccaca
    10801 acaacgcacg cagataaccc acggctttgt ccgccagagc cggatcgatg ttttccatgg
    10861 agcggataat gtaacctgca ttccacagca ccagcataat gagaacggaa acaaaacctg
    10921 ccagccagaa accttgtcgc acctgatgcg caatgcgctc acgtcgaccg gaaccattta
    10981 attgcgcgat aaccggcgtt aatgccagca gcagtccgtg accaaagagg atcgccggaa
    11041 gccagataga agtaccgata gcgaccgccg ccatgtcggt ggcactatag ccgcccgcca
    11101 tcacggtatc gacaaaaccc atcgcagttt gggcgatttg cgcgagaatc accgggattg
    11161 ctaatgctaa taacagacgc gcttcactga tatacttctg cacgtgaaca ccttttattt
    11221 gtagttatat gagagactaa aaagccgcct gaatgggcag caaaaagaag aagcagggga
    11281 aattcagtct attgtagcga ggatttatta attctccagt gaaaaaatag tcattcttac
    11341 cccttcactg gtaagggcgt ttgccacctg ctattgtgct gaacagaatg tcttaactga
    11401 tttcaggagt tgtaagtatg tttacgggga ttgtacaggg caccgcaaaa ctggtgtcga
    11461 ttgacgagaa accaaatttt cgtacgcatg tggtggagtt acccgaccac atgctggacg
    11521 gcctggaaac cggtgcgtcc gtggcgcata acggttgctg cctgaccgtg acggaaatta
    11581 acggcaacca tgtcagtttt gacctgatga aagaaacgtt acgcattacc aatcttggcg
    11641 atttaaaagt gggggattgg gtaaacgttg agcgtgcggc gaaattcagt gatgaaattg
    11701 gcggacactt aatgtcaggt catattatga ccactgctga agtggcgaaa atattaacct
    11761 cagaaaataa tcgccagatc tggtttaaag tccaggatag tcagttgatg aaatatattc
    11821 tgtacaaagg atttattggc atcgacggta ttagcctgac cgtcggcgaa gtcacgccaa
    11881 cgcgtttttg cgtccattta attccggaaa cactggaacg cacgactctt gggaagaaaa
    11941 aactcggcgc acgcgtcaac attgaaattg atccacaaac tcaggcagtg gtagatacgg
    12001 tagaacgtgt gctggcggca cgagaaaatg ccatgaatca accaggtaca gaagcctgat
    12061 ctgaactcgc ccccgaagag ggggcgatag aatagccttt agcgagcgac tcgaaggccg
    12121 ttttcaacac cgcgtgagaa caccacctgc cagagctgaa tatcacgggc gcggaaagca
    12181 cctgcacaag cattcagata ataggtaaac attcgtttaa agcgttcact gtagttatct
    12241 gcaatttctg gccatgcggc gaggaatcgt tcataccacg ccatcaacgt agtatcgtaa
    12301 tcagcaccga agttatgcca gtcttccatc acaaagtggg gttcgctgga ctgagcaatc
    12361 tggcgtacag agggcaggca accgttcgga aaaatatatt tattaatcca gggatcaaca
    12421 ttcagatcgg tttttttcga accgatagta tggagcagga atataccttc cggtttcaaa
    12481 ttacgatcca ccaccgcaaa ataggtatcg taatttttcg gtccgacgtg ctcgaacatc
    12541 cccacagaaa caatacgatc aaactggtcg ttcaggtcac gataatcttg cagcaaaatg
    12601 gtgacatcca ggccttcaca gcgttcctga gccatttttt gctgttcggc agaaatggtg
    12661 acgccgacca cgcttacgtc atagttagat gccatgtagt gcgccagtcc gccccagccg
    12721 cagccaatat ccagtacgcg catccctggt tttaactgca atttttcaca aatcattttg
    12781 agcttcgcct gctgggcaga ttccagatta tcggcatctt tccagtaagc gcaggaatat
    12841 tgcatgaagg gatcaagcat gcgactgaac aagtcattac ccaaatcgta atgctctttg
    12901 ccgactatcc aggcacgttt tttactctgc agattgaaga gacgggcgcc tgcaatacgc
    12961 agcgtgtctt tgaaatgatg ggggagttgg ttctcgagac ctgcgcgtaa gactttgcta
    13021 aaaaacatat ccagtcggtc acattcccac cagccatcca tataactttc gcctaacccc
    13081 aaagagcctt cttgcagaac gcgtttaaaa aaatcggggt ttttcacacg aatatccgcc
    13141 ggggcagaac cgttaatggc tataccggca cggctaagta attcgttggc gatacggtac
    13201 cagttgtcat ccggtacact gacttcttct atacacgatg aactcatagt ttctccatca
    13261 ctggtcggtg atcagaacct taaaacagcg tagacgcttt ttgggctttg tgagaaatct
    13321 cgcagaaaaa accgcgagtc tataatccga cgtagaacag agggaaagga ggaacccttg
    13381 ccgaaatgac catccatagt gagtcgggaa cgatcctgtc cccgtaaaaa cataatattt
    13441 atcgtagctt taacaactcg aaattagtat aggccgctaa tttcaatcat acaacaaaat
    13501 tttgttagca gcctaagtat aagtatatcg atatagatca gtgtgattcg ctatgagcga
    13561 cttcggcatt gccatgattc tggcagccaa cttcttcaca acgttgcatc atgtaaccca
    13621 gcgcgaccag cactactgtt gataacatca cgctggtggt ggtgagcaat ggcgtgctga
    13681 tactgatcag ccaggaaact accagacttg cgaggaagca cagacccagt tgcagagtgt
    13741 tctgcaacgc tgcggcgcga ccggttgcat gtgggaaggg acgcagcgcc tgggcgacaa
    13801 caatagggta gatcgcgcca ttggcaatcg ccatcacaca gaatgggatc aggatttcga
    13861 ccagcgacac atggctaatg aagcccgcag cccaggtcgc aatgacgctg acagcaaaca
    13921 gcaccagcaa ccacggtaat aactgcttgc cttgccattt ctgcagtgcg gcgcgacagc
    13981 cataaccacc aatcagaaac gcgatagttt gcgggacata acttaaacca ataactgccg
    14041 ggctgtagcc catttcacta aggatgaacg gtgaaccggt cagccatgca aaaaaactgg
    14101 ctgaacaggc tgcatatatc agcacgttgc cgcgataggt tttagaacgt agcaggtcgg
    14161 taaaggtcag accatcctga ctattgttac gggccttcgt cgtgggtttg agccagaaaa
    14221 taggcagaat cagcaccacg gtaatggcaa acagggtggc gaaaatcgcc tgccaggaaa
    14281 aatggaccag cagccagctt cctaacaggg gagccagtgc cggagacaga cccaccagcg
    14341 gcatgatggt cgcaaaaata cggttgactt tctgtgaagg ataataatct gtcactaatg
    14401 cttgccagat aaccgccgcg gcgcagaccc ccacagcctg tacaaaacgc aataccagca
    14461 gcgtagcggc gttttctacc cacagcatcc ccagactacc taacgcaaaa attgtcaggc
    14521 cgattaataa caccggttta cgaccataac ggtcggagag tggcccccac agaagctggg
    14581 ctgcggcaaa accggcaagg aacagactaa ggctggcact gacagcagac gcaggcgttt
    14641 gcaggtcggc ctgtatggcg gcgaaagcag gcagatacat atcggttgcc agaaaaccga
    14701 gtacgctcaa acccgccagc cagactaaaa atcttttccc aggttgcatg tatattctct
    14761 aaatctctca attaaacacc gccgcaaagt gtagggagtg aaattagtgt tgtgaaacgg
    14821 taatatttga tgactggttt caaaaatttt gcaggcagac tatgtggtca gaatattcac
    14881 tcgaagttgt tgatgcggta gcgcgtaatg gtagttttag cgctgcggca caggagctgc
    14941 atcgcgttcc ttctgcggtc agctataccg tgcgtcagct ggaagagtgg ctggcggtac
    15001 cgctctttga acgacggcac cgtgatgtgg agctgaccgc tgctggagcg tggtttctca
    15061 aagaagggcg ctctgttgtc aaaaaaatgc agatcacccg ccagcaatgt cagcagatag
    15121 cgaacggctg gcgcggtcag ttagctatcg cagtggataa tattgtcagg ccagaacgta
    15181 cacggcagat gatcgttgat ttttatcgcc attttgacga cgtcgaactt ctggtctttc
    15241 aggaagtgtt taacggtgtc tgggatgcgc tttccgatgg gcgcgtggaa ctggcgattg
    15301 gcgcaacacg cgcgattccg gtaggcggtc gttatgcctt ccgggatatg gggatgctaa
    15361 gctggagttg cgttgttgcc agccaccacc cgctggcgtt gatggatggc ccgttcagcg
    15421 atgatacgtt gcgcaactgg ccgtcgttgg tgcgcgaaga cacctcgcga acgctaccta
    15481 aacgtattac ctggttgctg gataaccaaa aaagagttgt cgtgcctgac tgggaatcat
    15541 cggcaacctg tatctcggca ggattatgca tagggatggt gccaacacat ttcgccaaac
    15601 cgtggcttaa tgaggggaag tgggtggcat tagaactgga aaaccccttt ccggattcgg
    15661 catgttgtct gacatggcag caaaatgata tgtcgcctgc gttaacctgg ctgcttgagt
    15721 atctcggcga tagtgagacg ctgaataagg agtggctgcg ggagccggaa gagactcccg
    15781 caacgggtga ttaacgacga tagtcgcgga acgggccgtc agccacggag cggcgttcaa
    15841 tcaagcgcgg atgcacttca atggactgcg gttcttcacg tttgttgacg atacgatcca
    15901 acagcatgtt gaacgctgtt tcacccagcg aatcttttgg ctggtggatc gtggtcagcg
    15961 ccggcgtaaa atagcgggcg ttgcgcacgt tatcataacc gatcagcgaa acatcctgcg
    16021 ggacgcgcag acccatttca tcagcagcac aaagtgcgcc cattgccatg atatcgccac
    16081 cacagaagac ggcagtaggg cgatgcggct gcgacagtat ttgctgcatg gcgcgataac
    16141 cagattcggg ttcaaagtca ccctgaacaa tccagctttc cggcaccttg atcatcgctt
    16201 cttccatcgc cttcataaaa ccggcaaggc ggcctgcgcc ggtgttacgt tccagcgggc
    16261 cggggatgac gccgatttcg cggtgaccgc gttcaatcag ataacgcccg gccatgtagc
    16321 cgccttcgaa cgcgttatca atgaccgcat cggtgaagtc agcttttgct tcaccccagt
    16381 ccatcaccac cattgggata tggcgatact cttccagcat cgccagcaac ggctctgggt
    16441 actcagaaca catcaccagc agaccatcga cgcgtttttg cgccatcatc gacagataag
    16501 cccgctgttt ctcaagattg ttccacgcat tacccagaat cagggtgtaa cctttctgga
    16561 agcaattttt ttcaaccgct tcaatgatct cggcaaaata ggccgcttcg ctgctggtcg
    16621 ccagcaaacc gatagacttg gtgtggttaa ccttcaggct acgcgccacc gcgctagggg
    16681 agtagtgtaa ttctttaatc gctgcccaca cggcgttgcg cgtttcttca gcgacgaaac
    16741 gtgttttgtt gattacgtgt gacacagttg tagtggaaac gtttgctcgt ttcgctacat
    16801 cttttattgt tgccattcca tttcactcca gaccctaact tcagctcctg caaaatcgca
    16861 aggtaaacgt ttgcctttac acaccttacg cacaacaaaa gtgttgcggt acgccggaaa
    16921 aacgacacgg agcgtcagga aggggtcaat ggccagtacg caaatagtgt aagcgtggaa
    16981 ttttggccga tcttgacgaa aaggggaagt ggtaaaaaca cttatcatta taaatgacgg
    17041 gagatttttt catcacagtg tgtaaaaatg cgtaatatca ctatttgtgt ggtaataaaa
    17101 aaggagaaaa cttgatgagc accgacctta aattttcact ggtaacaacg attatcgtcc
    17161 tcggtttgat cgtggccgtg ggtttgactg ccgcgctgca ctgatttcct ctcgagggag
    17221 cgcgttctcc ctcgcatctc cttcctgttt gttagcaaaa tggcaaaagt gttttctctt
    17281 tttgttattt tcattttttt gtgattgctg tcatgctttc gccactttaa tgaatatcgc
    17341 gtaatgacgc gaagaggtgg agataaggca tgaaaattaa ctatccgttg ctggcgctgg
    17401 cgattggcgc gtttggtatc gggacaacgg agttctctcc aatgggtttg ttgcccgtca
    17461 ttgcgcgcgg tgtggatgtc tcgattcccg ctgccggaat gttaatcagt gcctatgcag
    17521 ttggcgtaat ggttggcgcg ccgctgatga cgcttctact ttctcatcgt gcccgccgca
    17581 gtgcgttgat tttcctgatg gcaattttca cgctcggcaa cgtactttcc gccatcgcgc
    17641 cggattatat gaccctgatg ctttcacgca ttttgaccag cctgaatcac ggagcatttt
    17701 ttggtttggg ttcagtcgtg gccgcaagcg tggtgccaaa acataaacag gccagcgcag
    17761 ttgccactat gtttatgggg ttaaccctgg caaatatcgg tggcgtgccg gcggcgacct
    17821 ggttgggtga aaccatcggc tggcggatgt catttctggc aacggcgggg ctgggagtga
    17881 tttcaatggt aagtctgttc ttctcattac ctaaaggtgg tgcaggggca cgacctgaag
    17941 tgaaaaaaga gctggcggta ttaatgcgtc cgcaggtgct gtctgcattg ctgacgacgg
    18001 tactgggagc tggtgcaatg tttactctct acacctatat ctctccggta ctacaaagta
    18061 ttacccacgc aacaccagtg ttcgtcacgg caatgctggt gctgattggt gtcggattct
    18121 ctatcggtaa ctatctcggc ggcaaactgg cagatcgttc agttaacggc acgttgaaag
    18181 gctttttgtt gttgttgatg gtgattatgc tggcaatccc gttcctggcc cgcaatgagt
    18241 tcggcgcagc tattagcatg gtggtgtggg gcgcagcaac ctttgcggtc gtaccgccgt
    18301 tacagatgcg cgtgatgcgt gtcgccagtg aagcgccagg tctgtcttca tcagtcaata
    18361 ttggtgcctt taatcttgga aatgcgctgg gagcagctgc tggtggtgcg gtaatttccg
    18421 ctgggctggg atacagcttt gtgccggtga tgggagcgat tgtcgcggga ctggcattat
    18481 tgctggtgtt tatgtcagcc agaaaacaac ctgaaacagt ttgcgttgct aacagctaat
    18541 taaacaaaaa cgcagaagga taaccttctg cgttacagtt gctgacaacg tgcgatttat
    18601 cttgccgaat gcgctgttaa cgcctcatcc gcgttggcat ggtaagcgaa gcgcatcagg
    18661 caatgctgca tttgccatca gttattatgc agcgagattt ttcgctacga attcccagtt
    18721 caccagcgcc cagaagtgct ccagatagcc aggacgtgca ttgcgatagt cgatgtaata
    18781 agcgtgttcc cagacatcaa cggtcagcag cggagtcgca tcggtggtca gcggagtacc
    18841 cgcgttagag gttgaaacga tagccagttt gccatcgctg tttttcacca gccaggtcca
    18901 gccagaacca aagtttttga tcgctgcatc agtaaactgc gctttgaaat cggcaaagct
    18961 accaaaagat gcggcgatag cttcagcgac ttttccagtc ggttcgccac cggcgttcgg
    19021 tgccaggcag ttccagtaga aagtatggtt ccagacctga gctgcgttgt tgaatacgcc
    19081 accttcagag ctgcgaataa tctcttccag tgatttacct tcaaacgcgg tacctttaat
    19141 caggttgttc aggttagtga cataagtctg atgatgcttg ccgtagtgat actcgatagt
    19201 ttccgcagaa atgtgcggtg ccagagcatc tttagcatat ggtagtgcag gtaattcgaa
    19261 tgacattgct actctccttt attattaatt tgcatacgta caatagcctt attgtgcgta
    19321 tggatagggt agcaaattaa aaggtgagac aaaagatgaa cttaccctgt tgccgtaaca
    19381 acagggtaaa gttttagcga agtgtttttg gggtcattac ccgacgagcg ccaacatagt
    19441 ggcgctgcca gtagtcttca ctgagagaag tgatttgaat ttcctgacct gtgcgcggtg
    19501 actgaataaa tttgccgttg ccgacataca cgccgacatg atcggctgtg ccgcgtccct
    19561 gagtacggaa aaagaccagg tcgccgtttt tcagttcact acgttcgatt ggcgctgcat
    19621 cacgcaggtg atacatttca ttcgccgtac gcggaatacg aattttcacc aaatctttat
    19681 aagcgtaata aaccaggccg ctgcaatcaa aaccggtacg cggtgagctg ccaccccaac
    19741 gatatggctt accaatttgc tgcatcagtt tattcattgc cactttcgta gctttttgca
    19801 ccttcgcttt gtgcgcgtcg gcaagagttc ctgacgcggc atttttgact ttcacacaat
    19861 gcgatttata gcccttacgt ttggtacact tttcggtaaa gctggctgag gccgttttat
    19921 tgacggcgtg tttgctgcga gttttggcgt ttttagaagc ggtaatggaa gattttttaa
    19981 cggaagaagc ggttttgctt ttggtcgtac ttttactggc ggcttttttc gctgttttct
    20041 ggctggcttt ttgggtcttt ttggtggttg ccgtgctttt ctttttatct gcttttttgg
    20101 tgatatgagt ggtagcagaa ctctccctgg cttgctttga agcatgggcc ataggcgtta
    20161 aaggcagggt ggtaaaaagt aaagcacaga gcgtgatcga aatacggttt atccgcgcca
    20221 ctgagcgtcc ctctgttaag gcaggtctat catgcctgcg tctgatttac aaaacttgat
    20281 gattctaatg catagaagaa gagaaagtta atagctttta ttgttaaaaa atacgtttcg
    20341 ttggcgagcg caaaaaaatg cagcagtgaa agctaaaaat ggttataaat acgccagttt
    20401 gccgagcatc tgggaagaaa cgaatattta aaatgcaaca aatagagcaa agggataaaa
    20461 tagcaaaagc gtgatgaaaa tgttattttc cgatgccgca ctgacccgct acaatgtcag
    20521 attactgccg taaagaagtt tgaggaagca agacaatgag caccactatc gaaaaaatcc
    20581 aacgccagat tgctgaaaac ccgatcctgc tgtacatgaa aggttcaccg aaactgccga
    20641 gctgcggttt ctctgcccag gcagtccagg cgcttgccgc atgtggcgaa cgttttgcct
    20701 atgttgatat tctgcagaac ccggacattc gtgcggaact gcctaaatat gctaactggc
    20761 cgaccttccc gcaactgtgg gttgacggcg agctggtagg cggttgtgat atcgtgatcg
    20821 aaatgtatca gcgtggcgaa ctgcagcagc tgatcaaaga aactgccgct aaatacaagt
    20881 ctgaagagcc ggacgcggaa taatccgtcc tgcaatactg acggatgcga acgggcatcc
    20941 gtcagtcctt tctctatccc caatccagcc cttgtggcga acttgaaaat cccgcctcac
    21001 gcagcagcgt aaacatcggc gtttgccgga ccggtagatc attcacttct gttagcgtaa
    21061 agcgcagccg tggttcgcga cgcagtgcgg tagtcagcgc gtggaaaacc tctggggcga
    21121 gtaattcctc tttttcctgc cagaccagca tttttttgcc accttgcgcc agatagagta
    21181 acaatttgcc gccagaaacg accaccagcg cacccgcccg acgcgttgga accagcgttg
    21241 ccggatgtgc aggccagggc agaagatttc cccacacatt agccggatcg ttggcagaga
    21301 gcgccactgg tgtatagtgg cgcgtttgcg tggcttgtgt cgccagatcg cgcaatcggt
    21361 caatagtcag acgttcagcg aattgcgcgc cacccagacc ttctacaaaa cgacctcgca
    21421 taattcgccc ggagtcttcc atacttcgac aaagcgtttg catcgatgga aacccgtcag
    21481 ggatattttc ggctatcacc gcctgacgac tgatgatgcc gtagcggtcg agcatatttt
    21541 ccgccagcgc cagcatcctt tcggtatcgt ttagtggctc cacctgcaat aacgaccagc
    21601 gtccagccag atttggtgtg ttgtaagata ccagcggcga gacgggttgc gcatagacag
    21661 gacgtccacg acgagcccgg tgactgcggc gagttgaggt gcgtgcgttg gaactgctgc
    21721 gggtgagggc gcgtaacggt gcccaaatgt cgctggtgat gacgccttgc cagaccagcg
    21781 cccataacgc ctcttgcagg gcagagagat caaccgattc gccgattttg tcgcgtatcc
    21841 gttggctgat ttgttgtgca aaccaggctc ctccgtcagc cagaacagcg attatcgctt
    21901 gctgcagcgc cgaacgattc gcctgatccg cttcggcggg agtgaacgat tctgcggcat
    21961 attcctgtag atgcagtgcc accaggccgt catcttcacc cagctttttt tgtcccgacc
    22021 agataaccgc tccggttgcc agtaattcat cgagcatttc tggcgaatag tcgcgtacgc
    22081 gggcaggcag gatctggctt tcccagagtg aggcgggtaa accgactccg gcaagctgtt
    22141 cgatcacccg catcacgcca tctacgccct cataaacgcc tggcgatgtt gaggcaaaga
    22201 gcgccgggct accatcggtg gcgggtaata cgccctgacg ttccagcagc aatcgcgcat
    22261 aggtcgtggc tgcaacggga cgcgtcgctt ctctggcggc ttgcagcgag cgcaaacgca
    22321 gacgacgaaa tacttcatcg ctcacccaga tgtcttgttg cagattcatc accagaccct
    22381 gttcacgcag ttgctgaagc tgctcttcga caatggcaat accgagacta aactcatgag
    22441 ccagttgttc agccgtgacc agagcatgag cccggagata gcgcagaaag aggtcgcgaa
    22501 gcgggtaact gactctatgt aaataaatct ctggcaatga ctctggtagt cgtacgccga
    22561 gggcatcacg cagcctggcg gcatcatcca tacaggcaag acgctcctgt ccgctaatca
    22621 tcgctgggaa gattcgtttt actgcaagaa gattttccag atacgacgca acctcttcac
    22681 tgctgcctgt atgccgttgc gccaggtctt caacggtcat tggccccagt tcgcgcagca
    22741 ggtcgaacaa tccttcttca cctttcgctc ttctgccagg agccagtcgt tgcaactctt
    22801 cttccacctg gcgaatgacc tgcgggtcga gtaattcccc cggatcgacc tgtccgagta
    22861 gattgcgcag taactcgctg tccagcgaca gtacggatgc ccggcgctct gccagcgggg
    22921 cgtcgctctg gtacataaat tccgcgacat agccgaacaa taaacttgtg gcgaaaggcg
    22981 agggcgtagt ggtcgttaca tcggatattt gaatttcgcc accgttcagg cgacgcatca
    23041 aacgttccag tgcgggaaga tcataaacat cttgcagaca ttcgcgtagg gtttcgagaa
    23101 tgaccggaaa atccggatat ccctgagcga tttccagcaa ctgactggcg cgcagccgtt
    23161 gttgccaaag cggggtgcga tggcccggag tgcgccccgg cattaataat gcccgcgcgg
    23221 tgcattcgcg aaaacgggcg gcgaaaagtg ccgagctgcc taccgcctcg cggacaattt
    23281 gcagcaactt ttctggttca aacaaaaaaa tcgcggcgtc gggcaattta ccatcagtgt
    23341 caggaatgcg cgcaacaatg ccgtcatcac tggcgaccac agacgcgtca gcgccccata
    23401 gggcatgtat tcgcccggca atcgccagcg cccagggttc atgcacccgt cttccatagg
    23461 gagagtgcaa aataatacgc cagtcgccaa tttcatcacg gcaccgttcg aggaccagat
    23521 ggcgactccc cggaacgatg cccgtcgcgt tgcgttgctc gtcaatcaac ccctgaatat
    23581 tggcgttcgt attttcttct gccagccacg ggggaatagt cccggaaaag aacgcgccat
    23641 ccgccagtaa atgaagaaaa tcgccgatca tctcgcctaa ttcagccgga cgtccgttac
    23701 cttcaccacg ccagaagggg agccgggcag aacgacccgg agcaggagtc acaatcacct
    23761 gatcgcgggt gatttgctgg atccgccatg aggtagcgcc gagagtgata atgtcgctca
    23821 cccgcgactc atataccatc tcctcatcca gttcacccac ccgccgcgaa ccggcctgtt
    23881 cttcaccttc gggtaataac acgctataca tgccacgatc cggaatggtg ccgccgctgg
    23941 taaccgccaa caattgagcg ccaggtcggg cggtcaatat cccggtctcc ctgttccaga
    24001 ccagtttggg gcgaaaagca gaaaaatcgc cagagggata gcgcccggaa agcatatcca
    24061 gcgtggcgtc aaaaacacgt cttggcagat ctttccacgg tgcggcacgg cgtacgcggg
    24121 agtaccattc gtctacctgt aatgcatcca tcgccgcggc ggcaacggtt tgctgcgcaa
    24181 ggacgtcgag tggattatgc ggcggtgtca ggttttccag cctgcctgcg aacatacact
    24241 ctacaatgac tgcggaatcg actaagtcac gccgggtacg agggaaaaac agccctttag
    24301 atacaccgcc aacctgatgt ccggcgcgac caatgcgttg taacccactg gcaacagaaa
    24361 gcggcgttgc cacctgaatc accagatcca ccgcgcccat atcaatcccc agttcgagac
    24421 tggaggttgc gaccacgcag cgtaattcac ccgatttcag cgcctgttcg gtgattgctc
    24481 gttgttcttt agagacggag ccgtggtgtg agcgggcaat aaaaacgtcg ctactttgta
    24541 catggttaga ggttgcgccg gaggtcgact cgaaatgggc cgtatcaacg gcgatagacg
    24601 gggaacgctg taagcgtgcg gcgtaaagct catttaatcg tgccgtcagt ttttccgcca
    24661 gcccacgtga attagtaaag acaatggtcg agcgatggcg caacacttca tcaaggatac
    24721 ccgtttcaat atatggccag atggagcctt cccggccggc atggctgtct tcgccggtgc
    24781 cgctggcgac cgatgagaca tcatccatat tggcgaccgg tacgacaatt cgtatctgcg
    24841 gatggcgcat tgcgggcggg ttgactaccg taaccgggcg atcgccacca agaaatgctg
    24901 ccacatcgct ggctgagcgc acagtggcag aaaggccaat tcgctgtgct gaggtgtgga
    24961 gcagcgcatc gagccgctcc agacttaacg ccagatgcgc accacgttta ctgcccgcta
    25021 ccgcgtggac ttcatcaata attaccgttt cgacgccgcg tagcgtttcg cgcgcgcggg
    25081 aggtcagcat cagatagagt gattcgggtg tggtgatcag aatatccggc ggattacggg
    25141 tgagtttgct gcattcctgt gcaggcgtat cgccagtacg gatccctacg cgaagattga
    25201 cttccgtttc gccgcgccgc cgccgttcat cggcaatacc cttcaacggg agctgcaagt
    25261 tgcgctgaac gtcggtgccc agagctttta tcggtgaaat atagaggatg cgtgaggttt
    25321 tacgcttatg cgcctcgcgg gtatcttcgc cgccttcgcg gaagagccga tcgagggcgt
    25381 agagaaatgc tgccagcgtt ttcccggagc cggtcggtgc aatcaccagc gcatgttcgc
    25441 ttcgcgccgc cacatgccag gtttgcgact ggacagcggt cggctgttta aaggcgcgaa
    25501 gaaaccagtc gcgggtcgcc ggtgaaaaca cgtccggcag gagcgatgaa gggtctggat
    25561 tatctgccat taacgctatt ccccgtgaat atcatgcttg atgcgtggaa tagcctcact
    25621 atagatgcct gaatcattgc atgtcatcag gcatcgactc gattacacct cttcggcggc
    25681 aggtagcggc cagcctccca gacgtttcca gcggttgacg atttcacaaa acagcacagc
    25741 agtgcgttcg gtgtcgtaca gcgcggagtg cgcctgggtg ctgtcgaagt ccatgccagc
    25801 ggtctggcaa gcctttgaca atacggtttg tccgagtgcc agcccggcca gtgcagcagt
    25861 gtcaaaagtg gcgaaagggt ggaacgggtt acgtttcagt gaggcgcgtt ctgcggcggc
    25921 catcataaag ctgtgatcaa aattggcatt gtgcgccacc ataatggcgc ggttacagcc
    25981 gctcgcttta atacctttac gtacaacttt aaaaatttcg tgcagcgcct cgtattcgct
    26041 gaccgcgccg cgatcgggat cgttcgggtc aatgccgttg aaggcgaggg cctctggctg
    26101 caaatttgcg ccgacaaatg gttcgacgtg gaaatgtaat gtggtgtccg gcatcagcca
    26161 gccttgttca tccattttta gggtgatggc ggcaatctca agcagcgcat cggttttggc
    26221 gttaaatccg gctgtttcaa catcgatcac aacaggataa aaaccacgaa aacggtcgca
    26281 cagaccggta agttgagcgt tatcggacat ctgattctct taatacggat aaattgcagc
    26341 gcgcattatg acaaatatta cttgtcgatg cagtaaagat gcgggcgcga tgagttcacg
    26401 cccggcagga gattagttgc ccagaccgcg accggcgtct ttctcttcga ttaactcaat
    26461 tttgtaaccg tccggatctt ccacaaacgc gataaccgta gtaccgcctt ttaccggacc
    26521 cgcttcacgg gtcacgttac ccccgttttg acggattttt tcgcacgctt cagcggcgtt
    26581 atctacgcta agcgcgatgt gaccataagc agtgccgagt tcgtatttat ccacgcccca
    26641 gttgtaggtc agttcaatca ccgcttcttc ggtttccggg ccgtagccaa caaacgccag
    26701 tgagtatttg tattccgggt tttcgctggt acgcagcagt ttcatgccca gcactttggt
    26761 ataaaaatcg atggagcgtt gcagatcgcc aacgcgcagc atggtatgaa gaagacgcat
    26821 ctttttatcc tcaaaatggc taacagattc aaaatgttgt tttagtatag cggcggcttt
    26881 acgccgctcg caatgttgga ttacaacgtc gggtaatcgg tatagccttc cgcgccgcca
    26941 ccgtagaaac tttcggcacg ctgtgggtta agctcagctt tgcgctgcaa gcgggcgacc
    27001 agatccgggt tcgcaatcca gtcacgacca aatgccaccg catcaattaa ccctttgccg
    27061 atcagcgttt cagctttttc tactgtgtat gcacctgcgc cgataatcgg accgtggaaa
    27121 cgggcgcgta ctttttcgcg gaacgcatca gtatacggtt caccccccgc ccaatctggt
    27181 tctgacatat gcagataagc aatgccgcgt ttacccagtt gttcaatcag atacagtgca
    27241 tcggcttctt cattcgcgcc gttatccgtg ttctggaaag taccgattgg cgaaacgcga
    27301 atgccaatgc gatcggcacc ccattcttca atcccggcat cgaccacttc cagtaccaga
    27361 cgtgcgcgat tttccacgct gccgccgtac tgatcggtac gatggtttga agaaggagaa
    27421 aggaactgat gcagcaaata accgtgagca gagtggagct ctaccagatc aaaaccggct
    27481 tcacgcgcgt tagcaatggc ctgacggaaa tcattgacga tacctggaat ctcttccagt
    27541 tcaagcgcac gcggcatgga tgtttcaaca cggatcgcct gaccattttc atcgcgcaga
    27601 gaagtacgtg ttcccgcgct aagtgctgaa ggcgctaccg gtgcctgacc gccaggttgc
    27661 aggctggcgt gagaaatgcg tccggtgtgc cacagctgca cggccatatg accattttca
    27721 gcatgaacgc cagcggtgat ttttttccat gcggcaattt gctccggact atggatgcca
    27781 ggcgcacctg catatccttt tgcctgggca gaaatttgcg tggcttcact aataatcaaa
    27841 ccggcactgg cacgttggcg atagtattcc gccatcaacg gggtaggaat gtcacccggt
    27901 tcaatactgc gcagacgcgt cagcggtgcc ataaaaatac ggtttgccgc cgtgatcgcg
    27961 cccactttca gtggggaata cagtttttca gatgacataa aggctcccgg tcgtctggtg
    28021 ataaaaaatg cctgctaaac ggcaggcgtc gcaataatgt tttttacatg ggccagcgcg
    28081 ttttccagtg gctcgaaact gcgcgaaatt ttggcctgca gattcgcacc cagccatagt
    28141 gcgtaaagca cttgtgcctg ttgtagcggt tcgccacaaa aggttaaaca atggttctca
    28201 cggccatttt ccagcgcctg agagagcagg gcgatcacgc cgcgagcacc tttatccatc
    28261 gcgctgcgca tatcttctga cagatcgcac acttcggcag agagttttac tgtcaggcaa
    28321 ccactgatgg ttccatgttg gcaaaactgg ttcagtgttt gctggtaata agccagtatg
    28381 cggtcgcggt agttaccttc gccggattgc agcaactcag tcagtcgctg gtgatatgcg
    28441 gcgtaatgac gctcaagcat ggcaacgcca aacgcttctt tagagcgaaa gtagtgatag
    28501 aaggaccctt tcggcacttc agcggttttt agtaattcgc ttagccccat cccggtgaat
    28561 ccacgttgca ggcaaagttg ctcgcccgtc gccaggagat gttcgcgagt atcatgttcg
    28621 gtgtgtttgt tcatgcgttg gagtgtagta gaccagtcgg tctagtgcaa gcaaagttgc
    28681 tactttggac attaacagct tcaataatgg taataatctt atctgaggag ggcgaagtgg
    28741 cggagcaatt agagttcttt cccgtccaga gcccgtgccg gggaatttgc cagtctgacg
    28801 aacgcgggtt ttgtcgcggc tgttttcgta gtcgtgatga gcgttttaac tggaataaaa
    28861 tgagcgatgg cgaaaaacaa gaggtgctgc gcctttgtcg gcagcgatta atgcgcaaat
    28921 tacgcgcaaa taagccagcg tcatcagacg aaccggagca accatcactc ttttgaacct
    28981 aatcttgcgt atactcctct catatctgat aagaggaagt gattatggtt cagcgtatta
    29041 ctattgcgcc gcaaggcccg gagttttccc gttttgtgat gggctactgg cgattgatgg
    29101 actggaatat gtccgcccgc cagctggtca gttttattga agagcatctg gatctcggcg
    29161 tgaccaccgt ggaccatgct gatatttatg gtggctatca gtgcgaagcg gcgtttggcg
    29221 aggcactgaa actggcacct cacctgcgtg aacggatgga aatcgtcagt aaatgcggta
    29281 tcgcgacgac cgcgcgtgaa gaaaacgtca ttggtcatta catcactgac cgcgatcaca
    29341 tcattaagag cgccgaacag tcgctaatta atctcgcgac cgatcatctg gatctgctgt
    29401 taatccaccg accagacccg ttaatggatg ccgatgaagt ggcggacgcg ttcaaacatc
    29461 tgcatcagag cggcaaagtg cgtcattttg gcgtatcgaa ctttacgcct gcgcaatttg
    29521 ccctgttgca atcacgtctg ccgtttaccc ttgccactaa tcaggtggaa atatccccgg
    29581 tgcatcagcc gttactgctg gatggcacgc tcgaccaact acaacaactg cgtgttcgtc
    29641 cgatggcgtg gtcctgcctt ggtggtggtc gtctgtttaa tgatgattat ttccagcagc
    29701 tgcgtgatga actggctgtg gtggcagagg agttaaacgc gggctcgatt gaacaggtgg
    29761 tttacgcctg ggtattacgt ttaccatcgc agccgctgcc aattatcggt tcaggtaaaa
    29821 ttgagcgcgt acgggcagct gtcgaagcag aaacactgaa aatgacccgt caacaatggt
    29881 ttcgtatccg taaagcggca ctggggtacg acgtaccgta agcactttta ggaatagccg
    29941 ccgttcaaaa atgtgtcact ggtttacact tattcaggaa tgcacaatga acggaggtcc
    30001 tatgaaacgt tttagtctgg ctattctggc gctggttgtt gcaaccggcg cacaagctgc
    30061 cagtgaaaaa gtcgagatga acctcgtcac gtcgcaaggg gtagggcagt caattggtag
    30121 cgtcaccatt actgaaaccg ataaaggtct ggagttttcg cccgatctga aagcattacc
    30181 ccccggtgaa catggcttcc atattcatgc caaaggaagc tgccagccag ccaccaaaga
    30241 tggcaaagcc agcgccgcgg aatccgcagg cgggcatctt gatccacaaa ataccggtaa
    30301 acatgaaggg ccagaaggtg ccgggcattt aggcgatctg cctgcactgg tcgtcaataa
    30361 tgacggcaaa gctaccgatg ccgtcatcgc gcctcgtctg aaatcactgg atgaaatcaa
    30421 agacaaagcg ctgatggtcc acgttggcgg cgataatatg tccgatcaac ctaaaccgct
    30481 gggcggtggc ggtgaacgct atgcctgtgg tgtaattaag taattaagag gccagcgtac
    30541 cttgcggtgg tgcttgctca agttgcgaaa gcgagcagta cagccgccag acaattgccg
    30601 ccagctcacg ggcggcaggt tgatgatgac gggcaaggct gtcgcaaatc cgctgtaatt
    30661 cttcaagtgt ggccgccagt gatttttgct gaacgccacg ctcactcatc actccccgca
    30721 acagtgaaat acagttatcc cgtactcgcg ataacggatc ggaacgcgat tcccagtcgc
    30781 gcaattgcca gacaacatga gaacagttca gcagcactac accccagcgt aataaccaac
    30841 ggcgagccag cgcatcctgg ctgttactca actgactgac gtgatgataa gtgagcgatt
    30901 caaattcgct ttcactcagt gttggatggc ggcttagctg atcgacaaaa tcccggcgca
    30961 gcgcgcgaat atggcggcgg cttttacgag catccgatcc tggacgcaga atggcgaacg
    31021 ctagccaagc cagcgcaacg ccaacgattt ttgccagatt atcgttaaga aaatcagcaa
    31081 aatcatacac cgggggatta gtgacagcga taaacgaacc cataaaaaca atcagttgcc
    31141 cccacagtgc ggcaaatttg ggcatctgca atttaagaag ctgcattgtc gccagcagtg
    31201 gaaagagaaa cagtaaaaat tgccacagat cgctaatctg gaccatcagg ccaaatttga
    31261 ccacaaagct gaatagcgaa agtaacacca gcgtgcgcat cagcagtgac aacgacttaa
    31321 acggtgctgc gacggcggag tagagtacgc agctaattgc tgccagcgtt aatgcattgg
    31381 caccggcatc ccattgcgat gcaatactcc atgcgccaat catcatcaac gtacaaaatg
    31441 tacgcagccc gctccacata gcttcggcgt tgtcggtatg acgagccaga gcggatgtcc
    31501 gtgcgagtct ggcatgatca tctacaccgc tttgcagacg atgtaattcc tgactacttt
    31561 gcagataaag gcggcaaaaa taacgtagtc gctgccagaa ggcgacgtgc cggtagtcgg
    31621 cgacgttggt cgggcgtagc ggggcgataa tacgtgcgac ggtgtaagca tctgtttgcg
    31681 aactggcgag cgccgtcagc aattgttcga gaatttctcg cgtggcacct ggcggtgagg
    31741 gccagttgag caacatacgt cgcaggctgg agatgacact ggtcatacgt aattgctggt
    31801 ggagtagcgc attaaggcgc gcgttttgct ggcgaaaacg atagtggctc cagaaagcct
    31861 ggatacgcag caaattcatg gtcagtatct gcccaatcac cccctcatgt gctgcacgaa
    31921 tggcatcgtt tgtttcaggc tgccagagta aactggcatg ttccagtaat cgggcgtgca
    31981 tgtttttcaa tgcggttaaa agggctgtag catcggaact gctaggcagg atcatcatca
    32041 tcatgccgcc gcacaaaata ccgactatca cctcgcaaac gcgcgcctga gcgatatccc
    32101 acagctggct ggcctcagta atattaacca tcggaaaggc gatgatggca gccgtgtagc
    32161 ccgccagttg aaatgcatac gcgacgttat tcgtgaagtg cgcacaggcc caggtacaaa
    32221 agccaagcca cgccgacatg ctcaatagaa aaaaccacgg ctcattgagc gtatgcccgg
    32281 caagaagcag tgccgcaatg gctcccagta aactgccagc gatgcgtccg aggcttttgc
    32341 tgatgacacc gccaacggtg ggaaagctaa ccactgcagc cgaggtcatc gcccaatagg
    32401 gttcatccag attcaaataa taggcaaccg tcagcgccag acacatggca atggtattgc
    32461 gtaacgcata acgccattgc gccagcgtgg ccttgaacca gggcaaattg cgcaaggacc
    32521 atgacgatgc gttcattatt gacctacggc aatgctacag gtggtgcccg acaccagcgt
    32581 gacatcgcct ggaactttat ccagcgcaaa gcggacggga acgcgttggg cgagacgaac
    32641 ccaggggacg ttaggtttca catccggaat aaggcttgag tcactttcaa cgctttgatc
    32701 ataaatcgcc cgaccgatac tggaaacgtg accccgtaac gttttgttat cactatagag
    32761 ggtaatttgt gctggtgcac cttcgcggat atggcgcaac ttggtttctt caaaataacc
    32821 aatgacgtaa aacgagtggc tgtcgacaag ggcaaacagc ggtttcccgg tgtcagcgta
    32881 atcgccgatg cgggtagtga ggttagtcac ccaaccactc accggggcgc gaatttccgt
    32941 ttgcgccagt tgccattgtg tctgtttcag cgtggcctgt gcggcattaa cactggcctg
    33001 catcgcttta acattgaggt tagcggtatc gagttcttcg gcagaaataa aattttgtga
    33061 gagatgacga cgacgattcg cttcgttatt ggcttttgcg aggtcagatt gcgctttcgc
    33121 cagctgagcc tgtgcgttca gctcggcgat ctgaaagggc gttttatcga tcgtgagcag
    33181 aagatccccc gcgttaacca gttggttgtc ttttatattc agctcaacaa tacgtcccga
    33241 cacctgtggg gtgatagaaa ccttttctgc gcgtattttc ccgtcgcgtg tccacggaga
    33301 ttgcatgtaa taattccaca gccaccatcc ggcaagaacc gcaactatcg ctacaatgat
    33361 tgttgagaaa tacttaattg ttttaattga catagttacc acgcaatcag tattgccaga
    33421 gcaaggcaaa cgcaaatcgc aaacagcgat aaatccatta acaagggatg ccagatgtca
    33481 ccggcgtaga tccagccacg aagcaggcgg tgtacgacaa gccagatgac gaatccaaac
    33541 gcgaaggctt tgaaaaacgg aggaaaatag acggacgcac cgaacaccag atcttgtaag
    33601 ggcaatcctg ttgcattgag cataaacttc acaaagtaga ttcctttacg accgaatata
    33661 ttgcgttaga ttaataaata ttctttaagt gcgaaaaatt tacgcgcaat ttctgaaaaa
    33721 tatgtctgac ggtaaccaaa tgcagcaata catttgtttt agcaatacaa ttgctgcaca
    33781 ctattctaaa agccgcataa tatcttagca agctaattat aaggagatga aattggaatc
    33841 gccactaggt tctgatctgg cacggttggt gcgcatatgg cgtgctctga tagaccatcg
    33901 cctgaaaccg ctggagttaa cacaaaccca ttgggttacg ttacacaata tccatcagtt
    33961 acctccagac cagtcgcaaa ttcaactggc aaaagcgatt ggcatcgagc agccatcact
    34021 ggtccgtact ctggaccaac tggaagaaaa agggttaatt tcgcgtcaaa cttgtgccag
    34081 cgatcgtcgg gctaaacgta ttaaactgac ggaaaaggca gagccactga tcagcgaaat
    34141 ggaagctgtt attaacaaaa cccgcgcgga aatattacat ggcatctccg cagaggaact
    34201 ggagcaactg attacgctca tcgcaaaact tgagcataat atcattgagt tacaggccaa
    34261 agggtgaaat gaagggggcg tgtggtcagt aacctgacca cacgcaaact tatttagcgc
    34321 ggagaaacgg tcacctgact gccattgctg gccagtacga cacgttggcc cggagagaaa
    34381 cgagtgttgc cttgtttctg taccaccatg atggtattac catcgtcttt acgaatttcc
    34441 agctcgacac cctgcgtttt gttcattgca ctctgtacgc cctgaccagc tacgccacct
    34501 gcaacagcgc ctgctgcagt agccagagaa cgcccggttc cgccaccaac agtattcccc
    34561 aggaaaccac caagaacagc accgccaatt gcaccgataa cgttggaatc atcaccgccc
    34621 tgaatctgta ccggacgtac gttaacgatg gtgccatagc tgacattctg tacttgtttc
    34681 gcttcagaag cggtataaac atcccctgac agggtgtcgt tattaacaca accgacaaga
    34741 gacagaccta ccattgaaac aaccaatacg cgtttaatca ttgaaaaatc tcctgttcac
    34801 catgaaacgc cacgcaagca tccctcatgg tcaaagtata tggcatatgt gaatccatgg
    34861 ttacatgttc taccaaatat gagcaaatca taatgaaagt aggatcaacc aattataaac
    34921 aaaacattca tgatgattat tgatttgaag ataatgtttt actaccaacg gtaaaaatcg
    34981 gtcattccgc atggtattca cgaaaagttt atgtttgagt ggtgcgcaaa cgtcagattg
    35041 aaaaaggatg aactatgaaa tcgggccgct ttattggcgt tatgtcaggc accagccttg
    35101 atggtgttga tgttgtgttg gcgacaatag atgaacaccg ggtcgcacag ctggcaagct
    35161 tgagctggcc gatcccggtg tctctgaaac aggctgtact ggatatttgc cagggccagc
    35221 agcttacact ttcgcagttt ggacagcttg atactcaact cgggcgactt tttgctgatg
    35281 cggtcaatgc cttgcttaag gaacaaaacc tgcaggcgag agatatagtt gcgatcggct
    35341 gtcacggtca aaccgtctgg catgaaccga cgggcgtgga accacacact ttacagattg
    35401 gcgataacaa tcaaattgtg gcacgcaccg gaattacggt tgtcggtgat tttcgccgtc
    35461 gcgatattgc cttgggagga caaggcgcac cgctggtacc tgcgttccat catgccttgc
    35521 tggctcaccc aaccgagcga cgaatggtgc tcaatattgg cggcatcgcc aatctgtcac
    35581 tgctcattcc tgggcagccg gttggcggct acgataccgg tcctggtaac atgctgatgg
    35641 atgcctggat ctggcgtcag gccggtaaac cttacgataa agatgccgag tgggcacggg
    35701 cgggtaaagt tattctccca ctgctgcaaa atatgctcag cgacccatat ttctcgcaac
    35761 ctgcaccgaa aagcaccgga cgcgaatact ttaactacgg ctggctggag cgccatttac
    35821 gccattttcc gggtgttgat ccccgtgatg ttcaggccac actggcagaa ctcaccgccg
    35881 tgaccatttc tgaacaagtt ttgttgagcg gtggctgcga acgattgatg gtatgtggtg
    35941 gaggtagtcg taatccgcta ctcatggcgc gtctggcggc attactgcca ggcacagaag
    36001 tcaccaccac cgatgccgtt ggcattagtg gcgatgacat ggaagcattg gctttcgcct
    36061 ggcttgcctg gcggacgctg gcgggattac caggaaatct gccttccgtc actggcgcaa
    36121 gccaggagac ggtactgggg gctattttcc ctgctaaccc gtgatatcag agttaactga
    36181 atttttctta ccgcgtctgc cgttacactt ggaacgttag ggagggcgta ttgccctcca
    36241 gaccaggaaa gtcttcggga aatgtctatg aaaaaactgt tactgatctg tttgccggtg
    36301 cttctcaccg gctgtagcgc gttcaatcaa ctggttgagc acatgcaaac cgctacgctg
    36361 gaataccagt gtgatgaaaa accgttgacg gtcaaactga ataatccgcg ccaggaagtc
    36421 agttttgttt acgataatca actactgcat ctcaaacagg gcatttcagc gtctggtgca
    36481 cgttacactg acggaatcta tgttttctgg tcgaaaggcg atgaagcgac tgtctataaa
    36541 cgcgaccgca tcgtattgaa taactgtcag ttacaaaatc cacagcgttg agatttttcc
    36601 aggggcggcg cacaatagcg tcacccactg acaatccgta aagaaaacca tgtctgataa
    36661 cgacgaattg cagcaaatcg cgcatctgcg ccgtgaatac accaaaggcg ggttacgccg
    36721 tcgcgatctt cccgccgatc cattaaccct ttttgaacgt tggctctctc aggcgtgtga
    36781 agccaaactg gcagacccta ccgcgatggt ggtcgctacc gtggatgaac atgctcagcc
    36841 ttatcaacgc atcgttttac tcaaacatta cgacgaaaaa ggcatggtgt tttacaccaa
    36901 cctcggtagc cgtaaagcac atcaaattga aaataatccg cgcgttagcc tgctgttccc
    36961 gtggcatacc cttgaacgcc aggtgatggt gatcggtaaa gcagaacgac tttcgactct
    37021 cgaagtgatg aaatattttc atagccgccc gcgtgatagc cagattggtg catgggtttc
    37081 gaagcagtcc agtcgcattt ctgcccgcgg tatccttgaa agtaaattcc tggagctgaa
    37141 gcagaagttt caacagggcg aagtgccatt gcccagcttt tggggcggtt ttcgcgtcag
    37201 ccttgaacag attgagttct ggcagggcgg tgagcatcgc ctgcatgacc gctttttgta
    37261 ccagcgagaa aatgatgcgt ggaagattga tcgtcttgca ccctgaaaag atgcaaaaat
    37321 cttgctttaa tcgctggtac tcctgattct ggcactttat tctatgtctc tttcgcatct
    37381 ggcgaaaagt cgtgtaccgg caaaggtgca gtcgttatat acatggagat tttgatggca
    37441 agcagtaact tgattaaaca attgcaagag cgggggctgg tagcccaggt gacggacgag
    37501 gaagcgttag cagagcgact ggcgcaaggc ccgatcgcgc tctattgcgg cttcgatcct
    37561 accgctgaca gcttgcattt ggggcatctt gttccattgt tatgcctgaa acgcttccag
    37621 caggcgggcc acaagccggt tgcgctggta ggcggcgcga cgggtctgat tggcgacccg
    37681 agcttcaaag ctgccgagcg taagctgaac accgaagaaa ctgttcagga gtgggtggac
    37741 aaaatccgta agcaggttgc cccgttcctc gatttcgact gtggagaaaa ctctgctatc
    37801 gcggcgaaca actatgactg gttcggcaat atgaatgtgc tgaccttcct gcgcgatatt
    37861 ggcaaacact tctccgttaa ccagatgatc aacaaagaag cggttaagca gcgtctcaac
    37921 cgtgaagatc aggggatttc gttcactgag ttttcctaca acctgttgca gggttatgac
    37981 ttcgcctgtc tgaacaaaca gtacggtgtg gtgctgcaaa ttggtggttc tgaccagtgg
    38041 ggtaacatca cttctggtat cgacctgacc cgtcgtctgc atcagaatca ggtgtttggc
    38101 ctgaccgttc cgctgatcac taaagcagat ggcaccaaat ttggtaaaac tgaaggcggc
    38161 gcagtctggt tggatccgaa gaaaaccagc ccgtacaaat tctaccagtt ctggatcaac
    38221 actgcggatg ccgacgttta ccgcttcctg aagttcttca cctttatgag cattgaagag
    38281 atcaacgccc tggaagaaga agataaaaac agcggtaaag caccgcgcgc ccagtatgta
    38341 ctggcggagc aggtgactcg tctggttcac ggtgaagaag gtttacaggc ggcaaaacgt
    38401 attaccgaat gcctgttcag cggttctttg agtgcgctga gtgaagcgga cttcgaacag
    38461 ctggcgcagg acggcgtacc gatggttgag atggaaaagg gcgcagacct gatgcaggcg
    38521 ctggtcgatt ctgaactgca accttcccgt ggtcaggcgc gtaaaactat cgcctccaat
    38581 gccatcacca ttaacggtga aaaacagtcc gatcctgaat acttctttaa agaagaagat
    38641 cgtctgtttg gtcgttttac cttactgcgt cgcggcaaaa agaattactg tctgatttgc
    38701 tggaaataat gcattaagtg gaaaggggga gtgagaaatc actccccctg gtttttatac
    38761 agggaacatg atgaaaaata ttctcgctat ccagtctcac gttgtttatg gtcatgcggg
    38821 taacagtgcg gcagagtttc ctatgcgccg cctgggcgcg aacgtctggc cgctgaacac
    38881 cgttcaattt tctaatcaca cccaatacgg caaatggact ggctgcgtga tgccgcccag
    38941 ccatttaacc gaaattgtgc aaggcattgc cgccattgat aaattacaca cctgtgatgc
    39001 cgtgttaagt ggctatctgg gatcggcgga gcagggtgaa catatcctcg gtatcgtccg
    39061 tcaggtgaaa gccgcgaatc cgcaggcgaa atatttttgc gatccggtaa tgggtcatcc
    39121 ggaaaaaggc tgtatcgttg caccgggtgt cgcagagttt catgtgcggc acggtttgcc
    39181 tgccagcgat atcattgcgc cgaatctggt tgagctggaa atactctgtg agcatgcggt
    39241 aaataacgtc gaagaagcgg ttctggcagc gcgcgaactc attgcgcaag ggccacaaat
    39301 tgtgttggtt aaacacctgg cgcgagctgg ctacagccgt gaccgttttg aaatgctgct
    39361 ggtcaccgcc gatgaagcct ggcatatcag ccgtccgctg gtggattttg gtatgcgcca
    39421 gccggtaggt gttggtgatg tgacgagcgg tttactgctg gtgaaactgc ttcagggggc
    39481 aacgctgcag gaggcgctgg aacatgtgac cgctgcagtc tacgaaatca tggtgaccac
    39541 caaagcaatg caggaatatg agctgcaagt ggtggctgct caggatcgta ttgccaaacc
    39601 agaacattac ttcagcgcaa caaagctctg aaatatctca tttcggccca caacgacctg
    39661 tgggccgatg gatattactt taagccttcc gctgacagcg cgtcttgtac ttccggacgt
    39721 tcagccatac gttgcataaa tgctgcaatg tgctctaacc cttccagatt cagtttcacc
    39781 gcgtatgccc agcgcagaac cgtaaacaga taggcatcag caattgtaaa tctttgcccg
    39841 cagatccagt gctcatcctt cagtgcctcg ttcacatatt gcagcttctt ctccagctgc
    39901 gcgcgaactg tcggtttgta ctcttccggt gtatcagggc gaaacagagg tgtgaaacct
    39961 ttatgcagct cggtggcgat gtaattcagc cattcgatgg ttttatagcg ggaaatactg
    40021 tttaccggtg ccagcaactg gcggtcgggg acgctgtcgg caagatactg cataatcgct
    40081 acgccttccg tcagcaaagt accgtcatcc agcagcaatg caggcacctg ccccttaggg
    40141 ttaacggcaa agtaatcgtc accgttttcg agacgttttt tcattaaatc cacactgacg
    40201 agggtaaaat cctttccgct ctcacgcagg gtgatatggg aagcgagaga gcaggcaccc
    40261 ggtttgtaga acaatttcat cggtaactcc ttttgtctgt ggtttaaagt atggtagtgc
    40321 gttgctgaac aaaaaaaaag ccgctaacga aaagttagcg gcttgtaaaa gagtttccct
    40381 gaattttacg ctacagcagc tttcgccgct ttgtctgcag cgtcatcctg cgtcatacgg
    40441 tgcagtttag gcgcggtcag cagcatcagt actgcaataa tggcagtagc gacaccaatc
    40501 tgcaagaata cgcgaccata aacttccagt gacatcagcg ggtcggtaac gttatccggc
    40561 acagccatca tacccgcaac ataaccacca atcaggtttg caccggcagt ggtcaggaac
    40621 cagctaccca taatgaagcc catcagacgc tgcggaacga gttgtgcaac cattgccaga
    40681 cccagaccag agatcatcag ttccccgatg ctctgcaggc catagcttgc gaccagccag
    40741 cttacagaca cgataccagc gtcagacgcg aatttcgcac ccagcggcag aatcaggaac
    40801 gcaccagaac acatcaccat gccgattgca aacttggttg gcatcggcag ggtatcgccc
    40861 atcttgttat agatagcggc cagaatcgga ctaccgatga tgatccagaa cgggttcagt
    40921 gcctgatact gctcaggttc tacggccaga cccagaatgg agtgctcaac gttacgaatc
    40981 gcaaagaagt tcagtgatgt cggcatctgg ctgtacagca cgaagaagat gattgcttcg
    41041 agcatcagga tgaaggcaac gatcatttta cgacgcgcag cacctttcat cgcgaaggct
    41101 tctttaccga agataaccac gataccgaag gcaacaacgc ccagcgccat acgcgcaact
    41161 tcctgattgt gcagcagcca ggtggcgata gcgatcagtg ccacaacacc aataatggtt
    41221 agcagcaggt tacggtagtt gataggctcg aagtctggtt ttgaaccgta ctgtttaacc
    41281 cagcgttggc agaaggcgaa gttaacgata gtgatcagca ggcctacaac gctcaatgca
    41341 aacgcaacgc tccagccgta tttcgcggcc agccacggcg tagcaatcat agagaagaaa
    41401 gagccgatgt tgacggacat gtagtacatg gtgaatgcac cgtccagacg cgggtcgttt
    41461 ttctcatagc atgtagaaag cagagaagac gggttagcct taaacaggcc gttaccgacc
    41521 gcaatagccg ccatacccat ataaacaata ccggcgtcgt gaccagacca ggcaaccaga
    41581 gcataaccaa tcgccagcac aatagcgccg agcataatta cgcgtttagt acccagtacc
    41641 ttgtcaccta accagccgcc gatagcgacc agaccataaa ccagggcact aaaggaagag
    41701 aaaagggtga ttgaatccgc ttcagacata cccagttgtt taaccaggta aacagccata
    41761 attccttgta ggccgtaata accaaaacgt tcccataact caatcgagaa gatgagatag
    41821 aacgccttcg gttgtttgaa agcgttcaaa ctgacgcttt cagttggttt ttggtttgca
    41881 gtggacactt ttacctcttt ttttacatcc catattaacg ggggtgcttt ctcgcgttat
    41941 gtctaaaaaa catacgcgca attgttatag tgggagggga aacggcaggt aatgttcact
    42001 atcttgcact ttcagacaag tcctttgtaa taatctgtta catataactg aggcggggaa
    42061 ttcaaccatt ccgggaaatg ttatccagcg ttaaattttt caaaaaacgt aacggcagat
    42121 tttttcactg cactgagagg ggggtgatgg cttaacggcg atatgttctg ctatttagcg
    42181 tttttactag tgagatagtc cagtttctga aaaatagc
//
LOCUS       DAZKHF020000012        41452 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2201S31241
            SAMN43267800-rid25106413.denovo.012, whole genome shotgun sequence.
ACCESSION   DAZKHF020000012 DAZKHF020000000
VERSION     DAZKHF020000012.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267800
            Sequence Read Archive: SRR30307263
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 41452)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 41452)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 41452)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350581.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350581.2
            Genome Coverage       :: 151x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:21:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,652
            CDSs (total)                      :: 5,557
            Genes (coding)                    :: 5,320
            CDSs (with protein)               :: 5,320
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 237
            CDSs (without protein)            :: 237
            Pseudo Genes (ambiguous residues) :: 0 of 237
            Pseudo Genes (frameshifted)       :: 102 of 237
            Pseudo Genes (incomplete)         :: 130 of 237
            Pseudo Genes (internal stop)      :: 52 of 237
            Pseudo Genes (multiple problems)  :: 41 of 237
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..41452
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267800-rid25106413.denovo.012"
                     /serotype="O 26:H11"
                     /isolate="2201S31241"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-01"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            263..349
                     /gene="leuL"
                     /locus_tag="ACHOSC_RS02615"
                     /old_locus_tag="pgaptmp_000523"
     CDS             263..349
                     /gene="leuL"
                     /locus_tag="ACHOSC_RS02615"
                     /old_locus_tag="pgaptmp_000523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leu operon leader peptide"
                     /protein_id="HGU4546524.1"
                     /translation="MTHIVRFIGLLLLNASSLRGRRVSGIQH"
     gene            442..2013
                     /gene="leuA"
                     /locus_tag="ACHOSC_RS02620"
                     /old_locus_tag="pgaptmp_000524"
     CDS             442..2013
                     /gene="leuA"
                     /locus_tag="ACHOSC_RS02620"
                     /old_locus_tag="pgaptmp_000524"
                     /EC_number="2.3.3.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016831521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003852 - 2-isopropylmalate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0019752 - carboxylic acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-isopropylmalate synthase"
                     /protein_id="HGU4546525.1"
                     /translation="MSQQVIIFDTTLRDGEQALQASLSVKEKLQIALALERMGVDVME
                     VGFPVSSPGDFESVQTIARQVKNSRVCALARCVEKDIDVAAESLKVAEAFRIHTFIAT
                     SPMHIATKLRSTLDEVIERAIYMVKRARNYTDDVEFSCEDAGRTPIADLARVVEAAIN
                     AGATTINIPDTVGYTMPFEFAGIISGLYERVPNIDKAIISVHTHDDLGLAVGNSLAAV
                     HAGARQVEGAMNGIGERAGNCSLEEVIMAIKVRKDILNVHTAINHQEIWRTSQLVSQI
                     CNMPIPANKAIVGSGAFAHSSGIHQDGVLKNRENYEIMTPESIGLNQIQLNLTSRSGR
                     AAVKHRMDEMGYKESEYNLDNLYDAFLKLADKKGQVFDYDLEALAFIGKQQEEPEHFR
                     LDYFSVQSGSNDIATAAVKLACGEEVKAEAANGNGPVDAVYQAINRITDYNVELVKYS
                     LTAKGHGKDALGQVDIVANYNGRRFHGVGLATDIVESSAKAMVHVLNNIWRAAEVEKE
                     LQRKAQHNENNKETV"
     gene            2013..3104
                     /gene="leuB"
                     /locus_tag="ACHOSC_RS02625"
                     /old_locus_tag="pgaptmp_000525"
     CDS             2013..3104
                     /gene="leuB"
                     /locus_tag="ACHOSC_RS02625"
                     /old_locus_tag="pgaptmp_000525"
                     /EC_number="1.1.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003862 - 3-isopropylmalate dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0009098 - L-leucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydrogenase"
                     /protein_id="HGU4546526.1"
                     /translation="MSKNYHIAVLPGDGIGPEVMTQALKVLDAVRNRFAMRITTSHYD
                     VGGAAIDNHGQPLPPATVEGCEQADAVLFGSVGGPKWEHLPPDQQPERGALLPLRKHF
                     KLFSNLRPAKLYQGLEAFCPLRADIAANGFDILCVRELTGGIYFGQPKGREGSGQYEK
                     AFDTEVYHRFEIERIARIAFESARKRRHKVTSIDKANVLQSSILWREIVNEIATEYPD
                     VELAHMYIDNATMQLIKDPSQFDVLLCSNLFGDILSDECAMITGSMGMLPSASLNEQG
                     FGLYEPAGGSAPDIAGKNIANPIAQILSLALLLRYSLDADDAACAIERAINRALEEGI
                     RTGDLARGAAAVSTDEMGDIIARYVAEGV"
     gene            3107..4507
                     /gene="leuC"
                     /locus_tag="ACHOSC_RS02630"
                     /old_locus_tag="pgaptmp_000526"
     CDS             3107..4507
                     /gene="leuC"
                     /locus_tag="ACHOSC_RS02630"
                     /old_locus_tag="pgaptmp_000526"
                     /EC_number="4.2.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009316 - 3-isopropylmalate dehydratase
                     complex [Evidence IEA];
                     GO_function: GO:0003861 - 3-isopropylmalate dehydratase
                     activity [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0009098 - L-leucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydratase large subunit"
                     /protein_id="HGU4546527.1"
                     /translation="MAKTLYEKLFDAHVVYEAENETPLLYIDRHLVHEVTSPQAFDGL
                     RAHGRPVRQPGKTFATMDHNVSTQTKDINACGEMARIQMQELIKNCKEFGVELYDLNH
                     PYQGIVHVMGPEQGVTLPGMTIVCGDSHTATHGAFGALAFGIGTSEVEHVLATQTLKQ
                     GRAKTMKIEVQGKAAPGITAKDIVLAIIGKTGSAGGTGHVVEFCGEAIRDLSMEGRMT
                     LCNMAIEMGAKAGLVAPDETTFNYVKGRLHAPKGKDFDDAVAYWKTLQTDEGATFDTV
                     VTLQAEEISPQVTWGTNPGQVISVNDNIPDPASFADPVERASAEKALAYMGLKPGIPL
                     TEVAIDKVFIGSCTNSRIEDLRAAAEIAKGRKVAPGVQALVVPGSGPVKAQAEAEGLD
                     KIFIEAGFEWRLPGCSMCLAMNNDRLNPGERCASTSNRNFEGRQGRGGRTHLVSPAMA
                     AAAAVTGHFADIRNIK"
     gene            4518..5123
                     /gene="leuD"
                     /locus_tag="ACHOSC_RS02635"
                     /old_locus_tag="pgaptmp_000527"
     CDS             4518..5123
                     /gene="leuD"
                     /locus_tag="ACHOSC_RS02635"
                     /old_locus_tag="pgaptmp_000527"
                     /EC_number="4.2.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009316 - 3-isopropylmalate dehydratase
                     complex [Evidence IEA];
                     GO_function: GO:0003861 - 3-isopropylmalate dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009098 - L-leucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydratase small subunit"
                     /protein_id="HGU4546528.1"
                     /translation="MAEKFIKHTGLVVPLDAANVDTDAIIPKQFLQKVTRTGFGAHLF
                     NDWRFLDEKGQQPNPDFVLNFPQYQGASILLARENFGCGSSREHAPWALTDYGFKVVI
                     APSFADIFYGNSFNNQLLPVKLSDAEVDELFALVKANPGIHFDVDLEAQEVKAGEKTY
                     RFTIDAFRRHCMMNGLDSIGLTLQHDDAIAAYEAKQPAFMN"
     gene            complement(5172..6350)
                     /gene="setA"
                     /locus_tag="ACHOSC_RS02640"
                     /old_locus_tag="pgaptmp_000528"
     CDS             complement(5172..6350)
                     /gene="setA"
                     /locus_tag="ACHOSC_RS02640"
                     /old_locus_tag="pgaptmp_000528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_025293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar efflux transporter SetA"
                     /protein_id="HGU4546529.1"
                     /translation="MIWIMTMARRMNGVYAAFMLVAFMMGVAGALQAPTLSLFLSREV
                     GAQPFWIGLFYTVNAIAGIGVSLWLAKRSDSQGDRRKLIIFCCLMAIGNALLFAFNRH
                     YLTLITCGVLLASLANTAMPQLFALAREYADNSAREVVMFSSVMRAQLSLAWVIGPPL
                     AFMLALNYGFTVMFSIAAGIFTLSLVLIAFMLPSVARVELPSENALSMQGGWQDSNVR
                     MLFVASTLMWTCNTMYIIDMPLWISSELGLPDKLAGFLMGTAAGLEIPAMILAGYYVK
                     RYGKRRMMVIAVAAGVLFYTGLIFFHSRMALMTLQLFNAVFIGIVAGIGMLWFQDLMP
                     GRAGAATTLFTNSISTGVILAGVIQGAIAQSWGHFAVYWVIAVISVVALFLTAKVKDV
                     "
     gene            complement(6452..6583)
                     /gene="sgrT"
                     /locus_tag="ACHOSC_RS02645"
                     /old_locus_tag="pgaptmp_000529"
     CDS             complement(6452..6583)
                     /gene="sgrT"
                     /locus_tag="ACHOSC_RS02645"
                     /old_locus_tag="pgaptmp_000529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0046325 - negative regulation of glucose
                     import [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose uptake inhibitor SgrT"
                     /protein_id="HGU4546530.1"
                     /translation="MRQFYQHYFTATAKLCWLRWLSVPQRLTMLEELMQWDDRNSES"
     gene            6672..8327
                     /gene="sgrR"
                     /locus_tag="ACHOSC_RS02650"
                     /old_locus_tag="pgaptmp_000530"
     CDS             6672..8327
                     /gene="sgrR"
                     /locus_tag="ACHOSC_RS02650"
                     /old_locus_tag="pgaptmp_000530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706022.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator SgrR"
                     /protein_id="HGU4546531.1"
                     /translation="MPSARLQQQFIRLWQCCEGKSQDTTLNELAALLSCSRRHMRTLL
                     NTMQDRGWLTWEAEVGRGKRSRLTFLYTGLALQQQRAEDLLEQDRIDQLVQLVGDKAT
                     VRQMLVSHLGRSFRQGRHILRVLYYRPLRNLLPGSALRRSETHIARQIFSSLTRINEE
                     NGELEADIAHHWQQISPLHWRFFLRPGVHFHHGRELEMDDVIASLKRINTLPLYSHIA
                     DIVSPTPWTLDIHLTQPDRWLPLLLGQVPAMILPREWETLSNFASHPIGTGPYAVIRN
                     TTNQLKIQAFDDFFGYRALIDEVNVWVLPEIADEPAGGLMLKGPQGEEKEIESRLEEG
                     CYYLLFDSRTHRGANQQVRDWVSYVLSPTNLVYFAEEQYQQLWFPAYGLLPRWHHART
                     IKSEKPAGLESLILTFYQDHSEHRVIAGIMQQILASHQVTLEIKEISYDQWHEGEIES
                     DIWLNSANFTLPLDFSLFAHLCEVPLLQHCIPIDWQADAARWRNGEMNLANWCQQLVA
                     SKAMVPLIHHWLIIQGQRSMRGLRMNTLGWFDFKSAWFAPPDP"
     regulatory      8361..8460
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            8491..9474
                     /gene="thiB"
                     /locus_tag="ACHOSC_RS02655"
                     /old_locus_tag="pgaptmp_000531"
     CDS             8491..9474
                     /gene="thiB"
                     /locus_tag="ACHOSC_RS02655"
                     /old_locus_tag="pgaptmp_000531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706021.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0030975 - thiamine binding [Evidence IEA];
                     GO_function: GO:0048502 - ABC-type thiamine transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015888 - thiamine transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine ABC transporter substrate binding
                     subunit"
                     /protein_id="HGU4546532.1"
                     /translation="MLKKCLPLLLLCTAPVFAKPVLTVYTYDSFAADWGPGPKIKKAF
                     EADCNCELKLVALEDGVSLLNRLRMEGKNSKADVVLGLDNNLLDAASKTGLFAKSGVA
                     ADAVNVPGGWNNDTFVPFDYGYFAFVYDKNKLKNPPQSLKELVESDQNWRVIYQDLRT
                     STPGLGLLLWMQKVYGDDAPQAWQKLAKKTVTVTKGWSEAYGLFLKGESDLVLSYTTS
                     PAYHILEEKKDNYAAANFSEGHYLQVEVAARTAASKQPELAQKFLQFMVSPAFQNAIP
                     TGNWMYPVANVTLPAGFEQLTKPATTLEFTPAEVAAQRQAWISEWQRAVSR"
     gene            9450..11060
                     /gene="thiP"
                     /locus_tag="ACHOSC_RS02660"
                     /old_locus_tag="pgaptmp_000532"
     CDS             9450..11060
                     /gene="thiP"
                     /locus_tag="ACHOSC_RS02660"
                     /old_locus_tag="pgaptmp_000532"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine/thiamine pyrophosphate ABC transporter
                     permease ThiP"
                     /protein_id="HGU4546533.1"
                     /translation="MATRRQPLIPGWLIPGVSAATLVVAVALAAFLALWWNAPQGNWV
                     AVWQDSYLWHVVRFSFWQAFLSALLSVVPAIFLARALYRRRFPGRLALLRLCAMTLIL
                     PVLVAVFGILSVYGRQGWLASLCQSLGLEWTFSPYGLQGILLAHVFFNLPMASRLLLQ
                     ALENIPGEQRQLAAQLGMRGWHFFRFVEWPWLRRQIPPVAALIFMLCFASFATVLSLG
                     GGPQATTIELAIYQALSYDYDPARAAMLALLQMVCCLGLVLLSQRLSKAIAPGTTLLQ
                     GWRDPDDRLHSRICDTVLIVLALLLLLPPLLAVIVDGVNRQLPEVLAQPVLWQALWTS
                     LRIALAAGVLCVVLTMMLLWSSRELRARQKMLAGQALEMSGMLILAMPGIVLATGFFL
                     LLNNTIGLPQSADGIVIFTNALMAIPYALKVLENPMRDITARYSMLCQSLGIEGWSRL
                     KVVELRALKRPLAQALAFACVLSIGDFGVVALFGNDDFRTLPFYLYQQIGSYRSQDGA
                     VTALILLLLCFLLFTVIEKLPGRNVKTD"
     gene            11044..11742
                     /gene="thiQ"
                     /locus_tag="ACHOSC_RS02665"
                     /old_locus_tag="pgaptmp_000533"
     CDS             11044..11742
                     /gene="thiQ"
                     /locus_tag="ACHOSC_RS02665"
                     /old_locus_tag="pgaptmp_000533"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine ABC transporter ATP-binding protein
                     ThiQ"
                     /protein_id="HGU4546534.1"
                     /translation="MLKLTDITWLYHHLPMRFSLTVERGEQVAILGPSGAGKSTLLNL
                     IAGFLTPASGSLTIDSVDHTTTPPSRRPVSMLFQENNLFSHLTVAQNIGLGLNPGLKL
                     NAAQQEKMHAIARQMGIDNLMARLPGELSGGQRQRVALARCLVREQPILLLDEPFSAL
                     DPALRQEMLTLVSTSCQQQKMTLLMVSHSVEDAARIATRSVVVADGRIAWQGKTNELL
                     SGKASASALLGITG"
     gene            complement(11856..12620)
                     /gene="yabI"
                     /locus_tag="ACHOSC_RS02670"
                     /old_locus_tag="pgaptmp_000534"
     CDS             complement(11856..12620)
                     /gene="yabI"
                     /locus_tag="ACHOSC_RS02670"
                     /old_locus_tag="pgaptmp_000534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001148402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HGU4546535.1"
                     /translation="MQALLEHFITQSTVYSLMAVVLVAFLESLALVGLILPGTVLMAG
                     LGALIGSGELSFWHAWLAGIVGCLLGDWISFWLGWRFKKPLHRWSFLKKNKALLDKTE
                     HALHQHSMFTILVGRFVGPTRPLVPMVAGMLDLPVAKFITPNIIGCLLWPPFYFLPGI
                     LAGAAIDIPAGMQSGEFKWLLLATAVFLWVGGWLCWRLWRSGKATDRLSHYLSRGRLL
                     WLTPLISAIGVVALVVLIRHPLMPVYIDILRKVVGG"
     gene            complement(12706..13584)
                     /gene="araC"
                     /locus_tag="ACHOSC_RS02675"
                     /old_locus_tag="pgaptmp_000535"
     CDS             complement(12706..13584)
                     /gene="araC"
                     /locus_tag="ACHOSC_RS02675"
                     /old_locus_tag="pgaptmp_000535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arabinose operon transcriptional regulator AraC"
                     /protein_id="HGU4546536.1"
                     /translation="MAEAQNDPLLPGYSFNAHLVAGLTPIEANGYLDFFIDRPLGMKG
                     YILNLTIRGQGVVKNQGREFVCRPGDILLFPPGEIHHYGRYPEAREWYHQWVYFRPRA
                     YWHEWLNWPSIFANTGFFRPDEAHQPHFSDLFGQIINAGQGEGRYSELLAINLLEQLL
                     LRRMEAINESLHPPMDNRVREACQYISDHLADSNFDIASVAQHVCLSPSRLSHLFRQQ
                     LGISVLSWREDQRISQAKLLLSTTRMPIATVGRNVGFDDQLYFSRVFKKCTGASPSEF
                     RAGCEEKVNDVAVKLS"
     gene            13923..15623
                     /gene="araB"
                     /locus_tag="ACHOSC_RS02680"
                     /old_locus_tag="pgaptmp_000536"
     CDS             13923..15623
                     /gene="araB"
                     /locus_tag="ACHOSC_RS02680"
                     /old_locus_tag="pgaptmp_000536"
                     /EC_number="2.7.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008741 - ribulokinase activity [Evidence
                     IEA];
                     GO_process: GO:0019568 - arabinose catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribulokinase"
                     /protein_id="HGU4546537.1"
                     /translation="MAIAIGLDFGSDSVRALAVDCASGEEIATSVEWYPRWQKGQFCD
                     APNNQFRHHPRDYIESMEAALKTVLAELSVEQRAAVVGIGVDSTGSTPAPIDADGNVL
                     ALRPEFAENPNAMFVLWKDHTAVEEAEEITRLCHAPGNVDYSRYIGGIYSSEWFWAKI
                     LHVTRQDTAVAQSAASWIELCDWVPALLSGTTRPQDIRRGRCSAGHKSLWHESWGGLP
                     PASFFDELDPILNRHLPSPLFTDTWTADIPVGTLCPEWAQRLGLPESVVISGGAFDCH
                     MGAVGAGAQPNALVKVIGTSTCDILIADKQSVGERAVKGICGQVDGSVVPGFIGLEAG
                     QSAFGDIYAWFGRVLGWPLEQLAAQHPELKAQINASQKQLLPALTEAWAKNPSLDHLP
                     VVLDWFNGRRTPNANQRLKGVITDLNLATDAPLLFGGLIAATAFGARAIMECFTDQGI
                     AVNNVMALGGIARKNQVIMQACCDVLNRPLQIVASDQCCALGAAIFAAVAAKVHADIP
                     SAQQKMASAVEKTLQPRSEQAQRFEQLYRRYQQWAMSAEQHYLPTSAPAQAAQAVATL
                     "
     gene            15634..17136
                     /gene="araA"
                     /locus_tag="ACHOSC_RS02685"
                     /old_locus_tag="pgaptmp_000537"
     CDS             15634..17136
                     /gene="araA"
                     /locus_tag="ACHOSC_RS02685"
                     /old_locus_tag="pgaptmp_000537"
                     /EC_number="5.3.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008733 - L-arabinose isomerase activity
                     [Evidence IEA];
                     GO_process: GO:0005996 - monosaccharide metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-arabinose isomerase"
                     /protein_id="HGU4546538.1"
                     /translation="MTIFDNYEVWFVIGSQHLYGPETLRQVTQHAEHVVNALNTEAKL
                     PCKLVLKPLGTTPDEITAICRDANYDDRCAGLVVWLHTFSPAKMWINGLTMLNKPLLQ
                     FHTQFNAALPWDSIDMDFMNLNQTAHGGREFGFIGARMRQQHAVVTGHWQDKQAHERI
                     GSWMRQAVSKQDTRHLKVCRFGDNMREVAVTDGDKVAAQIKFGFSVNTWAVGDLVQVV
                     NSISDGDVNALVDEYESCYTMTPATQIHGEKRQNVLEAARIELGMKRFLEQGGFHAFT
                     TTFEDLHGLKQLPGLAVQRLMQQGYGFAGEGDWKTAALLRIMKVMSTGLQGGTSFMED
                     YTYHFEKGNDLVLGSHMLEVCPSIAVEEKPILDVQHLGIGGKDDPARLIFNTQTGPAI
                     VASLIDLGDRYRLLVNCIDTVKTPHSLPKLPVANALWKAQPDLPTASEAWILAGGAHH
                     TVFSHALNLNDMRQFAEMHDIEITVIDNDTRLPAFKDALRWNEVYYGFRR"
     gene            17305..18000
                     /gene="araD"
                     /locus_tag="ACHOSC_RS02690"
                     /old_locus_tag="pgaptmp_000538"
     CDS             17305..18000
                     /gene="araD"
                     /locus_tag="ACHOSC_RS02690"
                     /old_locus_tag="pgaptmp_000538"
                     /EC_number="5.1.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0008742 - L-ribulose-phosphate 4-epimerase
                     activity [Evidence IEA];
                     GO_process: GO:0019568 - arabinose catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 4-epimerase"
                     /protein_id="HGU4546539.1"
                     /translation="MLEDLKRQVLEANLALPKHNLVTLTWGNVSAVDRERGVFVIKPS
                     GVDYSVMTADDMVVVSIETGEVVEGTKKPSSDTPTHRLLYQAFPSIGGIVHTHSRHAT
                     IWAQAGQSIPATGTTHADYFYGTIPCTRKMTDAEINGEYEWETGNVIVETFEKQGIDA
                     AQMPGVLVHSHGPFAWGKNAEDAVHNAIVLEEVAYMGIFCRQLAPQLPDMQQTLLDKH
                     YLRKHGAKAYYGQ"
     gene            18075..20426
                     /gene="polB"
                     /locus_tag="ACHOSC_RS02695"
                     /old_locus_tag="pgaptmp_000539"
     CDS             18075..20426
                     /gene="polB"
                     /locus_tag="ACHOSC_RS02695"
                     /old_locus_tag="pgaptmp_000539"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase II"
                     /protein_id="HGU4546540.1"
                     /translation="MAQAGFILTRHWRDTPQGTEVSFWLATDNGPLQVTLAPQESVAF
                     IPADQVPRAQHILQGEQGFRLTPLALKDFHRQPVYGLYCRAHRQLMNYEKRLREGGVT
                     VYEADVRPPERYLMERFITSPVWVEGDMHNGTIVNARLKPHPDYRPPLKWVSIDIETT
                     RHGELYCIGLEGCGQRIVYMLGPENGDASSLDFELEYVASRPQLLEKLNAWFANYDPD
                     VIIGWNVVQFDLRMLQKHAERYRLPLRLGRDNSELEWREHGFKNGVFFAQAKGRLIID
                     GIEALKSAFWNFSSFSLETVAQELLGEGKSIDNPWDRMDEIDRRFAEDKPALATYNLK
                     DCELVTQIFHKTEIMPFLLERATVNGLPVDRHGGSVAAFGHLYFPRMHRAGYVAPNLG
                     EVPPHASPGGYVMDSRPGLYDSVLVLDYKSLYPSIIRTFLIDPVGLVEGMAQPDPEHS
                     TEGFLDAWFSREKHCLPEIVTNIWHGRDEAKRQGNKPLSQALKIIMNAFYGVLGTTAC
                     RFFDPRLASSITMRGHQIMRQTKALIEAQGYDVIYGDTDSTFVWLKGAHSEEEAAKIG
                     RALVQHVNAWWAETLQKQRLTSALELEYETHFCRFLMPTIRGADTGSKKRYAGLIQEG
                     DKQRMVFKGLETVRTDWTPLAQQFQQELYLRIFRNEPYQEYVRQTIDKLMAGELDARL
                     VYRKRLRRPLSEYQRNVPPHVRAARLADEENQKRGRPLQYQNRGTIKYVWTTNGPEPL
                     DYQRSPLDYEHYLTRQLQPVAEGILPFIEDNFATLMTGQLGLF"
     gene            20591..23497
                     /gene="rapA"
                     /locus_tag="ACHOSC_RS02700"
                     /old_locus_tag="pgaptmp_000540"
     CDS             20591..23497
                     /gene="rapA"
                     /locus_tag="ACHOSC_RS02700"
                     /old_locus_tag="pgaptmp_000540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001117023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016817 - hydrolase activity, acting on
                     acid anhydrides [Evidence IEA];
                     GO_function: GO:0140658 - ATP-dependent chromatin
                     remodeler activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase-associated protein RapA"
                     /protein_id="HGU4546541.1"
                     /translation="MPFTLGQRWISDTESELGLGTVVAVDARTVTLLFPSTGENRLYA
                     RSDSPVTRVMFNPGDTITSHDGWQMQVEEVKEENGLLTYIGTRLDTEESGVALREVFL
                     DSKLVFSKPQDRLFAGQIDRMDRFALRYRARKYSSEQFRMPYSGLRGQRTSLIPHQLN
                     IAHDVGRRHAPRVLLADEVGLGKTIEAGMILHQQLLSGAAERVLIIVPETLQHQWLVE
                     MLRRFNLRFALFDDERYAEAQHDAYNPFDTEQLVICSLDFARRSKQRLEHLCEAEWDL
                     LVVDEAHHLVWSEDAPSREYQAIEQLAEHVPGVLLLTATPEQLGMESHFARLRLLDPN
                     RFHDFAQFVEEQKNYRPVADAVAMLLAGNKLSNDELNMLGEMIGEQDIEPLLQAANSD
                     SEDAQSARQELVSMLMDRHGTSRVLFRNTRNGVKGFPKRELHTIKLPLPTQYQTAIKV
                     SGIMGARKSAEDRARDMLYPERIYQEFEGDNATWWNFDPRVEWLMGYLTSHRSQKVLV
                     ICAKAATALQLEQVLREREGIRAAVFHEGMSIIERDRAAAWFAEEDTGAQVLLCSEIG
                     SEGRNFQFASHMVMFDLPFNPDLLEQRIGRLDRIGQAHDIQIHVPYLEKTAQSVLVRW
                     YHEGLDAFEHTCPTGRTIYDSVYNDLINYLASPDQTEGFDDLIKNCREQHEALKAQLE
                     QGRDRLLEIHSNGGEKAQALAESIEEQDDDTNLIAFAMNLFDIIGINQDDRGDNMIVL
                     TPSDHMLVPDFPGLSEDGITITFDREVALAREDAQFITWEHPLIRNGLDLILSGDTGS
                     STISLLKNKALPVGTLLVELIYVVEAQAPKQLQLNRFLPPTPVRMLLDKNGNNLAAQV
                     EFETFNRQLNAVNRHTGSKLVNAVQQDVHAILQLGEAQIEKSARALIDAARNEADEKL
                     SAELSRLEALRAVNPNIRDDELTAIESNRQQVMESLDQAGWRLDALRLIVVTHQ"
     gene            23509..24168
                     /gene="rluA"
                     /locus_tag="ACHOSC_RS02705"
                     /old_locus_tag="pgaptmp_000541"
     CDS             23509..24168
                     /gene="rluA"
                     /locus_tag="ACHOSC_RS02705"
                     /old_locus_tag="pgaptmp_000541"
                     /EC_number="5.4.99.28"
                     /EC_number="5.4.99.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional tRNA pseudouridine(32) synthase/23S
                     rRNA pseudouridine(746) synthase RluA"
                     /protein_id="HGU4546542.1"
                     /translation="MGMENYNPPQEPWLVILYQDDHIMVVNKPSGLLSVPGRLEEHKD
                     SVMTRIQRDYPQAESVHRLDMATSGVIVVALTKAAERELKRQFREREPKKQYVARVWG
                     HPSPAEGLVDLPLICDWPNRPKQKVCYETGKPAQTEYEVVEYVADNTARVVLKPITGR
                     SHQLRVHMLALGHPILGDRFYASPEARAMAPRLLLHAEMLTITHPAYGNSMTFKAPAD
                     F"
     gene            complement(24285..25100)
                     /gene="djlA"
                     /locus_tag="ACHOSC_RS02710"
                     /old_locus_tag="pgaptmp_000542"
     CDS             complement(24285..25100)
                     /gene="djlA"
                     /locus_tag="ACHOSC_RS02710"
                     /old_locus_tag="pgaptmp_000542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051087 - protein-folding chaperone
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone DjlA"
                     /protein_id="HGU4546543.1"
                     /translation="MQYWGKIIGVAVALLMGGGFWGVVLGLLIGHMFDKARSRKMAWF
                     ANQRERQALFFATTFEVMGHLTKSKGRVTEADIHIASQLMDRMNLHGASRTAAQNAFR
                     VGKSDNYPLREKMRQFRSVCFGRFDLIRMFLEIQIQAAFADGSLHPNERAVLYVIAEE
                     LGISRAQFDQFLRMMQGGAQFGGGYQQQSGGGNWQQAQRGPTLEDACNVLGVKPTDDA
                     TTIKRAYRKLMSEHHPDKLVAKGLPPEMMEMAKQKAQEIQQAYELIKQQKGFK"
     gene            25355..27709
                     /gene="lptD"
                     /locus_tag="ACHOSC_RS02715"
                     /old_locus_tag="pgaptmp_000543"
     CDS             25355..27709
                     /gene="lptD"
                     /locus_tag="ACHOSC_RS02715"
                     /old_locus_tag="pgaptmp_000543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA];
                     GO_process: GO:0015920 - lipopolysaccharide transport
                     [Evidence IEA];
                     GO_process: GO:0043165 - Gram-negative-bacterium-type cell
                     outer membrane assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS assembly protein LptD"
                     /protein_id="HGU4546544.1"
                     /translation="MKKRIPTLLATMIATALYSQQGLAADLASQCMLGVPSYDRPLVQ
                     GDTNDLPVTINADHAKGDYPDDAVFTGSVDIMQGNSRLQADEVQLHQKEAPGQPEPVR
                     TVDALGNVHYDDNQVILKGPKGWANLNTKDTNVWEGDYQMVGRQGRGKADLMKQRGEN
                     RYTILDNGSFTSCLPGSDTWSVVGSEIIHDREEQVAEIWNARFKVGPVPIFYSPYLQL
                     PVGDKRRSGFLIPNAKYTTTNYFEFYLPYYWNIAPNMDATITPHYMHRRGNIMWENEF
                     RYLSQAGAGLMELDYLPSDKVYEDEHPNDDSSRRWLFYWNHSGVMDQVWRFNVDYTKV
                     SDPSYFNDFDNKYGSSTDGYATQKFSVGYAVQNFNATVSTKQFQVFSEQNTSSYSAEP
                     QLDVNYYQNDVGPFDTRIYGQAVHFVNTRDDMPEATRVHLEPTINLPLSNNWGSINTE
                     AKLLATHYQQTNLDWYNSRNTTKLDESVNRVMPQFKVDGKMVFERDMEMLAPGYTQTL
                     EPRAQYLYVPYRDQSDIYNYDSSLLQSDYSGLFRDRTYGGLDRIASANQVTTGVTSRI
                     YDDAAVERFNISVGQIYYFTESRTGDDNITWENDDKTGSLVWAGDTYWRISERWGLRG
                     GIQYDTRLDNVATSNSSIEYRRDEDRLVQLNYRYASPEYIQATLPKYYSTAEQYKNGI
                     SQVGAVASWPIADRWSIVGAYYYDTNANKQADSMLGVQYSSCCYAIRVGYERKLNGWD
                     NDKQHAVYDNAIGFNIELRGLSSNYGLGTQEMLRSNILPYQNTL"
     gene            27762..29048
                     /gene="surA"
                     /locus_tag="ACHOSC_RS02720"
                     /old_locus_tag="pgaptmp_000544"
     CDS             27762..29048
                     /gene="surA"
                     /locus_tag="ACHOSC_RS02720"
                     /old_locus_tag="pgaptmp_000544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_function: GO:0042277 - peptide binding [Evidence IEA];
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA];
                     GO_process: GO:0043165 - Gram-negative-bacterium-type cell
                     outer membrane assembly [Evidence IEA];
                     GO_process: GO:0050821 - protein stabilization [Evidence
                     IEA];
                     GO_process: GO:0060274 - maintenance of stationary phase
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase SurA"
                     /protein_id="HGU4546545.1"
                     /translation="MKNWKTLLLGIAMIANTSFAAPQVVDKVAAVVNNGVVLESDVDG
                     LMQSVKLNAAQARQQLPDDATLRHQIMERLIMDQIILQMGQKMGVKISDEQLDQAIAN
                     IAKQNNMTLDQMRSRLAYDGLNYNTYRNQIRKEMIISEVRNNEVRRRITILPQEVESL
                     AQQVGNQNDASTELNLSHILIPLPENPTSDQVNEAESQARAIVDQARNGADFGKLAIA
                     HSADQQALNGGQMGWGRIQELPGIFAQALSTAKKGDIVGPIRSGVGFHILKVNDLRGE
                     SKNISVTEVHARHILLKPSPIMTDEQARVKLEQIAADIKSGKTTFAAAAKEFSQDPGS
                     ANQGGDLGWATPDIFDPAFRDALTRLNKGQMSAPVHSSFGWHLIELLDTRNVDKTDAA
                     QKDRAYRMLMNRKFSEEAASWMQEQRASAYVKILSN"
     gene            29048..30037
                     /gene="pdxA"
                     /locus_tag="ACHOSC_RS02725"
                     /old_locus_tag="pgaptmp_000545"
     CDS             29048..30037
                     /gene="pdxA"
                     /locus_tag="ACHOSC_RS02725"
                     /old_locus_tag="pgaptmp_000545"
                     /EC_number="1.1.1.262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050570 - 4-hydroxythreonine-4-phosphate
                     dehydrogenase activity [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxythreonine-4-phosphate dehydrogenase
                     PdxA"
                     /protein_id="HGU4546546.1"
                     /translation="MVKTQRVVITPGEPAGIGPDLVVQLAQREWPVELVVCADATLLT
                     DRAAMLGLPLTLRPYSPNSPAQPQTTGTLTLLPVALRESVTAGQLAIENGHYVVETLA
                     RACDGCLNGEFAALITGPVHKGVINDAGIPFTGHTEFFEERSQAKKVVMMLATEELRV
                     ALATTHLPLRDIADAITPALLHEVIAILHHDLRTKFGIAEPRILVCGLNPHAGEGGHM
                     GTEEIDTIIPVLDELRAQGMKLNGPLPADTLFQPKYLDNADAVLAMYHDQGLPVLKYQ
                     GFGRGVNITLGLPFIRTSVDHGTALELAGRGEADVGSFITALNLAIKMIVNTQ"
     gene            30034..30855
                     /gene="rsmA"
                     /locus_tag="ACHOSC_RS02730"
                     /old_locus_tag="pgaptmp_000546"
     CDS             30034..30855
                     /gene="rsmA"
                     /locus_tag="ACHOSC_RS02730"
                     /old_locus_tag="pgaptmp_000546"
                     /EC_number="2.1.1.182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000179 - rRNA
                     (adenine-N6,N6-)-dimethyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (adenine(1518)-N(6)/adenine(1519)-N(6))-
                     dimethyltransferase RsmA"
                     /protein_id="HGU4546547.1"
                     /translation="MNNRVHQGHLARKRFGQNFLNDQFVIDSIVSAINPQKGQAMVEI
                     GPGLAALTEPVGERLDQLTVIELDRDLAARLQTHPFLGPKLTIYQQDAMTFNFGELAE
                     KMGQPLRVFGNLPYNISTPLMFHLFSYTDAIADMHFMLQKEVVNRLVAGPNSKAYGRL
                     SVMAQYYCNVIPVLEVPPSAFTPPPKVDSAVVRLVPHATMPHPVKDVRVLSRITTEAF
                     NQRRKTIRNSLGNLFSVEVLTGMGIDPAMRAENISVAQYCQMANYLAENAPLQES"
     gene            30858..31235
                     /gene="apaG"
                     /locus_tag="ACHOSC_RS02735"
                     /old_locus_tag="pgaptmp_000547"
     CDS             30858..31235
                     /gene="apaG"
                     /locus_tag="ACHOSC_RS02735"
                     /old_locus_tag="pgaptmp_000547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Co2+/Mg2+ efflux protein ApaG"
                     /protein_id="HGU4546548.1"
                     /translation="MINSPRVCIQVQSVYIEAQSSPDNERYVFAYTVTIRNLGRAPVQ
                     LLGRYWLITNGNGRETEVQGEGVVGVQPLIAPGEEYQYTSGAIIETPLGTMQGHYEMI
                     DENGVPFSIDIPVFRLAVPTLIH"
     gene            31242..32090
                     /gene="apaH"
                     /locus_tag="ACHOSC_RS02740"
                     /old_locus_tag="pgaptmp_000548"
     CDS             31242..32090
                     /gene="apaH"
                     /locus_tag="ACHOSC_RS02740"
                     /old_locus_tag="pgaptmp_000548"
                     /EC_number="3.6.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008803 -
                     bis(5'-nucleosyl)-tetraphosphatase (symmetrical) activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bis(5'-nucleosyl)-tetraphosphatase (symmetrical)
                     ApaH"
                     /protein_id="HGU4546549.1"
                     /translation="MATYLIGDVHGCYDELFALLHKVEFTPGKDTLWLTGDLVARGPG
                     SLDVLRYVKSLGDSVRLVLGNHDLHLLAVFAGISRNKPKDRLTPLLEAPDADELLNWL
                     RRQPLLQIDEEKKLVMAHAGITPQWDLQTAKECARDVEAVLSSDSYPFFLDAMYGDMP
                     NNWSPELRGLGRLRFITNAFTRMRFCFPNGQLDMYSKESPEEAPAPLKPWFAIPGPVA
                     EEYSIAFGHWASLEGKGTPEGIYALDTGCCWGGSLTCLRWEDKQYFVQPSNRHKDLGE
                     GEAVAS"
     gene            32115..32714
                     /locus_tag="ACHOSC_RS02745"
                     /old_locus_tag="pgaptmp_000549"
     CDS             32115..32714
                     /locus_tag="ACHOSC_RS02745"
                     /old_locus_tag="pgaptmp_000549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000796364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4291 domain-containing protein"
                     /protein_id="HGU4546550.1"
                     /translation="MADYFEIRADYNQHTITIYQAYNDAIADVAVRDGRFGAPFSFNR
                     MTWIKPSFMWMMERSNWGLKKDQQHILAIRIKRTFFDTLLEQAVLTTPEAHVYPHAGI
                     WETLFAQANVYVQWDPERSINGKKLEHRSLQLGISRNLISQFNEDAIVAIDDLTPLVR
                     KCHNLLINGKTTQAKSFLPPEKIYPVSAAARKALGMKNS"
     gene            32750..33217
                     /locus_tag="ACHOSC_RS02750"
                     /old_locus_tag="pgaptmp_000550"
     CDS             32750..33217
                     /locus_tag="ACHOSC_RS02750"
                     /old_locus_tag="pgaptmp_000550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001248983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4546551.1"
                     /translation="MRQINTGLNTREIILEMIDEADAPPFAEQRTWHRRRIAFLCDCI
                     SVAVDYGFFCSVSQQSIMNITKMAEDVWITESKTTQLIDIWQQLKQLYPPETIPYAQK
                     LPTKELLELLLSSPDDLRIIEIPDIIGDVFDDQTWIRLFKKHFADVIQLRCDV"
     gene            complement(33316..33495)
                     /locus_tag="ACHOSC_RS02755"
                     /old_locus_tag="pgaptmp_000551"
     CDS             complement(33316..33495)
                     /locus_tag="ACHOSC_RS02755"
                     /old_locus_tag="pgaptmp_000551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000998541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system CcdA family
                     antitoxin"
                     /protein_id="HGU4546552.1"
                     /translation="MNDKTFDFTSERDFVSRAHCVGLNLQATLQWNEVNEDAISALNE
                     FAEETGCFSDEYRKF"
     gene            complement(33516..33995)
                     /gene="folA"
                     /locus_tag="ACHOSC_RS02760"
                     /old_locus_tag="pgaptmp_000552"
     CDS             complement(33516..33995)
                     /gene="folA"
                     /locus_tag="ACHOSC_RS02760"
                     /old_locus_tag="pgaptmp_000552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020456510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004146 - dihydrofolate reductase activity
                     [Evidence IEA];
                     GO_process: GO:0046654 - tetrahydrofolate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type 3 dihydrofolate reductase"
                     /protein_id="HGU4546553.1"
                     /translation="MISLIAALAVDRVIGMENAMPWNLPADLAWFKRNTLNKPVIMGR
                     HTWESIGRPLPGRKNIILSSQPGTDDRVTWVKSVDEAIAACGDVPEIMVIGGGRVYEQ
                     FLPKAQKLYLTHIDAEVEGDTHFPDYEPDDWESVFSEFHDADAQNSHSYCFEILERR"
     gene            complement(34187..36049)
                     /gene="kefC"
                     /locus_tag="ACHOSC_RS02765"
                     /old_locus_tag="pgaptmp_000553"
     CDS             complement(34187..36049)
                     /gene="kefC"
                     /locus_tag="ACHOSC_RS02765"
                     /old_locus_tag="pgaptmp_000553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015503 - glutathione-regulated potassium
                     exporter activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-regulated potassium-efflux system
                     protein KefC"
                     /protein_id="HGU4546554.1"
                     /translation="MDSHTLIQALIYLGSAALIVPIAVRLGLGSVLGYLIAGCIIGPW
                     GLRLVTDAESILHFAEIGVVLMLFIIGLELDPQRLWKLRAAVFGGGALQMVICGGLLG
                     LFCMLLGLRWQVAELIGMTLALSSTAIAMQAMNERNLMVTQMGRSAFAVLLFQDIAAI
                     PLVAMIPLLATSSASTTMGAFALSALKVAGALVLVVLLGRYVTRPALRFVARSGLREV
                     FSAVALFLVFGFGLLLEEVGLSMAMGAFLAGVLLASSEYRHALESDIEPFKGLLLGLF
                     FIGVGMSIDFGTLLENPLRIVILLLGFLIIKIAMLWLIARPLQVPNKQRRWFAVLLGQ
                     GSEFAFVVFGAAQMANMLEPEWAKSLTLAVALSMAATPILLVILNRLEQSSTEEAREA
                     DEIDEEQPRVIIAGFGRFGQITGRLLLSSGVKMVVLDHDPDHIETLRKFGMKVFYGDA
                     TRMDLLESAGAAKAEVLINAIDDPQTNLQLTEMVKEHFPHLQIIARARDVDHYIRLRQ
                     AGVEKPERETFEGALKTGRLALESLGLGPYEARERADVFRRFNIQMVEEMAMVENDTK
                     ARAAVYKRTSAMLSEIITEDREHLSLIQRHGWQGTEEGKHTGNMADEPETKPSS"
     gene            complement(36042..36572)
                     /gene="kefF"
                     /locus_tag="ACHOSC_RS02770"
                     /old_locus_tag="pgaptmp_000554"
     CDS             complement(36042..36572)
                     /gene="kefF"
                     /locus_tag="ACHOSC_RS02770"
                     /old_locus_tag="pgaptmp_000554"
                     /EC_number="1.6.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-regulated potassium-efflux system
                     oxidoreductase KefF"
                     /protein_id="HGU4546555.1"
                     /translation="MILIIYAHPYPHHSHANKRMLEQARTLEGVEIRSLYQLYPDFNI
                     DIAAEQEALSRADLIVWQHPMQWYSIPPLLKLWIDKVFSHGWAYGHGGTALHGKHLLW
                     AVTTGGGESHFEIGAHPGFDVLSQPLQATAIYCGLNWLPPFAMHCTFICDDETLEGQA
                     RHYKQRLLEWQEAHHG"
     gene            complement(36680..38011)
                     /gene="yaaU"
                     /locus_tag="ACHOSC_RS02775"
                     /old_locus_tag="pgaptmp_000555"
     CDS             complement(36680..38011)
                     /gene="yaaU"
                     /locus_tag="ACHOSC_RS02775"
                     /old_locus_tag="pgaptmp_000555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HGU4546556.1"
                     /translation="MQPSRNFDDLKFSSIHRRILLWGSGGPFLDGYVLVMIGVALEQL
                     TPALKLDADWIGLLGAGTLAGLFVGTSLFGYISDKVGRRKMFLIDIIAIGVISVATMF
                     VSSPVELLVMRVLIGIVIGADYPIATSMITEFSSTRQRAFSISFIAAMWYVGATCADL
                     VGYWLYDVEGGWRWMLGSAAIPCLLILIGRFELPESPRWLLRKGRVKECEEMMIKLFG
                     EPVAFDEEQPQQTRFRDLFNRRHFPFVLFVAAIWTCQVIPMFAIYTFGPQIVGLLGLG
                     VGKNAALGNVVISLFFMLGCIPPMLWLNTAGRRPLLIGSFAMMTLALAVLGLIPDMGI
                     WLVVMAFAVYAFFSGGPGNLQWLYPNELFPTDIRASAVGVIMSLSRIGTIVSTWALPI
                     FINNYGISNTMLMGAGISLFGLLISVAFAPETRGMSLAQTSNMTIRGQRMG"
     gene            complement(38070..38357)
                     /gene="fixX"
                     /locus_tag="ACHOSC_RS02780"
                     /old_locus_tag="pgaptmp_000556"
     CDS             complement(38070..38357)
                     /gene="fixX"
                     /locus_tag="ACHOSC_RS02780"
                     /old_locus_tag="pgaptmp_000556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin-like protein FixX"
                     /protein_id="HGU4546557.1"
                     /translation="MTSPVNVDVKLGVNKFNVDEEHPHIVVKADADKQALELLVKACP
                     AGLYKKQDDGSVRFDYAGCLECGTCRILGLGSALEQWEYPRGTFGVEFRYG"
     gene            complement(38354..39640)
                     /gene="fixC"
                     /locus_tag="ACHOSC_RS02785"
                     /old_locus_tag="pgaptmp_000557"
     CDS             complement(38354..39640)
                     /gene="fixC"
                     /locus_tag="ACHOSC_RS02785"
                     /old_locus_tag="pgaptmp_000557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HGU4546558.1"
                     /translation="MSEDIFDAIIVGAGLAGSVAALVLAREGAQVLVIERGNSAGAKN
                     VTGGRLYAHSLEHIIPGFADSAPVERLITHEKLAFMTEKSAMTMDYCNGDETSPSQRS
                     YSVLRSKFDAWLMEQAEEAGAQLITGIRVDNLVQRDGKVVGVEADGDVIEAKTVILAD
                     GVNSILAEKLGMAKRVKPTDVAVGVKELIELPKSVIEDRFQLQGNQGAACLFAGSPTD
                     GLMGGGFLYTNENTLSLGLVCGLHHLHDAKKSVPQMLEDFKQHPAVAPLIAGGKLVEY
                     SAHVVPEAGINMLPELVGDGVLIAGDAAGMCMNLGFTIRGMDLAIAAGEAAAKTVLSA
                     MKSDDFSKQKLAEYRQHLESGPLRDMRMYQKLPAFLDNPRMFSGYPELAVGVARDLFT
                     IDGSAPELMRKKILRHGKKVGFINLIKDGMKGVTVL"
     gene            complement(39691..40632)
                     /gene="fixB"
                     /locus_tag="ACHOSC_RS02790"
                     /old_locus_tag="pgaptmp_000558"
     CDS             complement(39691..40632)
                     /gene="fixB"
                     /locus_tag="ACHOSC_RS02790"
                     /old_locus_tag="pgaptmp_000558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit
                     alpha/FixB family protein"
                     /protein_id="HGU4546559.1"
                     /translation="MNTFSQVWVFSDTPSRLPELMNGAQALANQINTFVLNDADGVQA
                     IQLGANHVWKLNGKPDDRMIEDYAGVMADTIRQHGADGLVLLPNTRRGKLLAAKLGYR
                     LKAAVSNDASTVSVQDGKATVKHMVYGGLAIGEERIATPYAVLTISSGTFDAAQPDAS
                     RTGETHTVEWQAPAVAITRTATQARQSNSVDLDKARLVVSVGRGIGSKENIALAEQLC
                     KAIGAELACSRPVAENEKWMEHERYVGISNLMLKPELYLAVGISGQIQHMVGANASQT
                     IFAINKDKNAPIFQYADYGIVGDAVKILPALTAALAR"
     gene            complement(40647..41417)
                     /gene="fixA"
                     /locus_tag="ACHOSC_RS02795"
                     /old_locus_tag="pgaptmp_000559"
     CDS             complement(40647..41417)
                     /gene="fixA"
                     /locus_tag="ACHOSC_RS02795"
                     /old_locus_tag="pgaptmp_000559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414583.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein FixA"
                     /protein_id="HGU4546560.1"
                     /translation="MKIITCYKCVPDEQDIAVNNADGSLDFSKADAKISQYDLNAIEA
                     ACQLKQQAAEAQVTALSVGGKALTNAKGRKDVLSRGPDELIVVIDDQFEQALPQQTAS
                     ALAAAAQKAGFDLILCGDGSSDLYAQQVGLLVGEILNIPAVNGVSKIISLTADTLTVE
                     RELEDETETLSIPLPAVVAVSTDINSPQIPSMKAILGAAKKPVQVWSAADIGFNAVDA
                     WSEQQVAAPKQRERQRIVIEGDGEEQIAAFAENLRKVI"
BASE COUNT        10022 a        11377 c        10842 g         9211 t
ORIGIN      
        1 taggttttgc aacgcgtgca ttttgtcccc tttttcctcg ttgattagat gcaaaaattt
       61 atgctgaaat atgtcaaccg atgaaaagcg tcggtagtta agcagaaatt aatatcgctt
      121 actttaacca ccgcagcaca attagctaat tttacgggtg cagaactcac gctggcggga
      181 cgtttttatt gcgtcagggt tgacatccgt ttttgtatcc agtaactcta aaagcatatc
      241 gcattcatct ggagctgatt taatgactca catcgttcgc tttatcggtc tactactact
      301 aaacgcatct tctttgcgcg gtagacgagt gagcggcatc cagcattaag ccagcacgca
      361 gtcaaacaaa aaacccgcgc cattgcgcgg gtttttttat gcccgaagcg aggcgctcta
      421 aaagagacaa ggacccaaac catgagccag caagtcatta ttttcgatac cacattgcgc
      481 gacggtgaac aggcgttaca ggcaagcttg agtgtgaaag aaaaactgca aattgcgctg
      541 gcccttgagc gtatgggtgt tgacgtgatg gaagtcggtt tccccgtctc ttcgccgggc
      601 gattttgaat cggtgcaaac catcgcccgc caggttaaaa acagccgcgt atgtgcgtta
      661 gctcgctgcg tggaaaaaga tatcgacgtg gcggccgaat ccctgaaagt cgccgaagcc
      721 ttccgtattc atacctttat tgctacctcc ccaatgcaca tcgccaccaa gctgcgcagc
      781 accctggacg aggtgatcga acgcgctatc tatatggtga aacgcgcccg taattacacc
      841 gatgatgttg aattttcttg cgaagatgcc gggcgtacac ccattgccga tctggcgcga
      901 gtggtcgaag cggcgattaa tgccggtgcc accaccatca acattccgga caccgtgggc
      961 tacaccatgc cgtttgagtt cgccggaatc atcagcggcc tgtatgaacg cgtacctaac
     1021 atcgacaaag ccattatttc cgtacatacc cacgacgatt taggcctggc ggtcggcaac
     1081 tcactggcgg cggtacatgc cggtgcacgc caggtggaag gtgcaatgaa cgggatcggc
     1141 gagcgtgccg gtaactgttc gctggaagaa gtcatcatgg cgattaaagt tcgtaaggat
     1201 attctcaacg tccacaccgc cattaatcac caggaaatat ggcgcaccag ccagttagtt
     1261 agccagattt gtaatatgcc gatcccggca aacaaagcca ttgttggcag cggcgcattc
     1321 gcacactcct ccggtatcca ccaggatggc gtgctgaaaa accgcgaaaa ctacgaaatc
     1381 atgacaccag aatctattgg tctgaaccaa atccagctga atctgacctc tcgttcgggg
     1441 cgtgcggcgg tgaaacatcg catggacgag atggggtata aagaaagtga atataattta
     1501 gacaatttgt acgacgcctt cctgaagctg gcggacaaaa aaggtcaggt gtttgattac
     1561 gatctggagg cgctggcctt catcggtaag cagcaagaag agccggagca tttccgtctg
     1621 gattacttca gcgtgcagtc tggctctaac gatatcgcca ccgccgccgt caaactggcc
     1681 tgtggcgaag aagtcaaagc agaagccgcc aacggtaacg gtccggtcga tgccgtctat
     1741 caggcaatta accgcatcac tgactataac gtcgaactgg tgaaatacag cctgaccgcc
     1801 aaaggtcacg gtaaagatgc gctgggtcag gtggatattg tcgccaacta caacggtcgc
     1861 cgcttccacg gcgtcggcct ggccaccgat attgtcgagt cctccgccaa agccatggtg
     1921 cacgtactga acaatatctg gcgtgccgca gaagtcgaaa aagagttgca acgcaaagct
     1981 caacacaacg aaaacaacaa ggaaaccgtg tgatgtcgaa gaattaccat attgccgtat
     2041 tgccggggga cggtattggt ccggaagtga tgacccaggc gctgaaagtg ctggatgccg
     2101 tgcgcaaccg ctttgcgatg cgcatcacca ccagccatta cgatgtaggc ggtgcagcca
     2161 ttgataacca cgggcaacca ctgccgcctg cgacggttga aggttgtgag caagccgatg
     2221 ccgtgctgtt tggctcggta ggcggtccga aatgggaaca tttaccacca gaccagcaac
     2281 cagaacgcgg cgcgctgttg cctttgcgta agcacttcaa attattcagc aacctgcgcc
     2341 cggcaaaact gtatcagggg ctggaagcat tctgtccgct gcgtgcagac attgccgcta
     2401 acggcttcga catcctgtgc gtgcgcgaac tgaccggcgg catctatttc ggtcagccaa
     2461 aaggccgcga aggtagcgga caatatgaaa aagcgtttga taccgaggtg tatcaccgtt
     2521 ttgagatcga gcgtattgcc cgcatcgcgt ttgaatctgc tcgtaaacgt cgccacaaag
     2581 tcacctcaat cgacaaagct aacgtgctgc aatcctctat tttatggcgt gaaatcgtca
     2641 acgagatcgc cacggaatac ccggatgtcg aactggcgca tatgtacatc gacaacgcca
     2701 ccatgcagct gattaaagat ccatcacagt ttgacgttct gctgtgctcc aacctgtttg
     2761 gcgacattct gtctgacgag tgcgcaatga tcactggctc gatggggatg ttgccttccg
     2821 ccagcctgaa cgagcaaggt tttggtctgt atgaaccggc aggcggctca gcaccagata
     2881 tcgcaggcaa aaacatcgcc aacccgattg cgcaaattct gtcgctggca ctgctgctgc
     2941 gctacagcct ggatgccgat gatgcggctt gcgccattga acgcgccatt aaccgcgcat
     3001 tagaagaagg cattcgcacc ggggatttag cccgtggcgc tgccgccgtt agtaccgatg
     3061 aaatgggcga tatcattgcc cgctatgtgg cagaaggggt gtaatcatgg ctaaaacgtt
     3121 atacgaaaaa ttattcgacg ctcacgtagt gtacgaagcc gaaaacgaaa ccccgctgtt
     3181 atatatcgac cgccacctgg tgcatgaagt gacctcaccg caggcgttcg atggtctgcg
     3241 cgcccacggt cgcccggtac gtcagccggg caaaaccttc gccactatgg atcacaacgt
     3301 ctctacccag acgaaagaca ttaatgcctg tggtgaaatg gcgcgcatcc agatgcagga
     3361 gctgattaaa aactgcaaag aatttggcgt cgaactgtat gacctgaatc acccgtatca
     3421 ggggatcgtc cacgtaatgg ggccggaaca gggcgtcacc ttgccgggga tgaccattgt
     3481 ctgcggcgac tcgcataccg ccacccacgg cgcgtttggc gcactggcct ttggtatcgg
     3541 cacttccgaa gttgaacacg tactggcaac gcaaaccctg aaacagggcc gcgcaaaaac
     3601 catgaaaatt gaagtccagg gcaaagccgc gccgggcatt accgcaaaag atatcgtgct
     3661 ggcaattatc ggtaaaaccg gtagcgcagg cggcaccggg catgtggtgg agttttgcgg
     3721 cgaagcaatc cgtgatttaa gcatggaagg tcgtatgacc ctgtgcaata tggcaatcga
     3781 aatgggcgca aaagccggtc tggttgcacc ggacgaaacc acctttaact atgtcaaagg
     3841 ccgtttgcat gcgccgaaag gcaaagattt cgacgacgcc gttgcctact ggaaaaccct
     3901 gcaaaccgac gaaggcgcaa ctttcgatac cgttgtcact ctgcaagcag aagaaatttc
     3961 accgcaggtc acctggggca ccaatccagg ccaggtgatt tccgtgaacg acaatattcc
     4021 cgatccggct tcgtttgccg atccggttga acgcgcgtcg gcagaaaaag cgctggccta
     4081 tatggggctg aaaccgggca ttccgctgac cgaagtggct atcgacaaag tgtttatcgg
     4141 ttcctgtacc aactcgcgca ttgaagattt acgcgcggca gcggagatcg ccaaagggcg
     4201 aaaagtcgcg ccaggcgtgc aggcactggt ggttcccggc tctggcccgg taaaagccca
     4261 ggcggaagcg gaaggtctgg ataaaatctt tattgaagcc ggttttgaat ggcgcttgcc
     4321 tggctgctca atgtgtctgg cgatgaacaa cgaccgtctg aatccgggcg aacgttgtgc
     4381 atccaccagc aaccgtaact ttgaaggccg ccaggggcgc ggcgggcgca cgcatctggt
     4441 cagcccggca atggctgccg ctgctgcagt gaccggacat ttcgccgaca ttcgcaacat
     4501 taaataagga gcacaccatg gcagagaaat ttatcaaaca cacaggcctg gtggttccgc
     4561 tggatgccgc caatgtcgat accgatgcaa tcatcccgaa acagtttttg cagaaagtga
     4621 cccgtacggg ttttggcgcg catctgttta acgactggcg ttttctcgat gaaaaaggcc
     4681 aacagccaaa cccggacttc gtgctgaact tcccgcagta tcagggcgct tccattttgc
     4741 tggcacgaga aaacttcggc tgtggctcct cgcgtgagca cgcgccctgg gcattgaccg
     4801 actacggttt taaagtggtg attgcgccga gttttgctga catcttctac ggcaatagct
     4861 ttaacaacca gctgctgccg gtgaaattaa gcgatgcaga agtggacgaa ctgtttgcgc
     4921 tggtgaaagc caatccgggg atccatttcg acgtggatct ggaagcgcaa gaggtgaaag
     4981 cgggagagaa aacctatcgc tttaccatcg atgccttccg ccgccactgc atgatgaacg
     5041 gtctggacag tattgggctt accttgcagc acgacgacgc cattgccgct tatgaagcaa
     5101 aacaacctgc gtttatgaat taatcccctt gcccggtcaa atgaccgggc tttccgctat
     5161 cgtccacgtc atcaaacgtc tttaaccttt gcggttaaaa ataatgcgac aacagaaata
     5221 accgcaatta cccagtagac agcaaagtgc ccccaacttt gtgcaattgc tccctgaata
     5281 acgccagcca gaattacccc ggtagaaata ctgttagtaa ataaggtggt agctgccccc
     5341 gctcttccag gcattaaatc ctgaaaccat agcatcccaa tacccgcaac aatgccgata
     5401 aatacagcgt taaaaagttg cagcgtcatc aacgccatac ggctatgaaa gaaaatcaat
     5461 ccggtgtaaa acagtactcc tgccgccact gctatgacca tcattcgccg cttaccataa
     5521 cgtttgacat agtagccagc cagaatcatt gctggtattt ccagtccagc tgccgtcccc
     5581 atcaggaaac ccgccagttt gtctggcaat cctaactcgc tactgatcca caacggcata
     5641 tcaataatgt acatggtgtt gcaggtccac attaacgtcg aggcgacaaa taacatccgt
     5701 acgttactat cctgccagcc accttgcatt gataaagcat tttccgacgg cagttctacc
     5761 cgcgccacag acggaagcat aaatgcaatc aataccagac tgagtgtgaa tatcccggcg
     5821 gcaatcgaaa acatcaccgt aaagccgtaa ttcaacgcca gcataaaggc caacggtgga
     5881 ccgataaccc atgccagaga aagctgcgca cgcatcaccg agctaaacat caccacttct
     5941 cgcgccgagt tatccgcata ttcccgcgcc agagcaaata actgtggcat tgccgtattg
     6001 gccagagatg ccagaagcac accacaggtg ataagcgtca gataatgacg attaaatgca
     6061 aacaatagcg cattgccgat agccatcaaa cagcaaaata taatcagttt tcgccgatcg
     6121 ccctgactgt cagaacgttt tgccaaccag aggcttacgc cgatcccagc aatagcattc
     6181 accgtataaa agagtccgat ccagaaaggt tgcgcgccaa cctcacgact cagaaataag
     6241 ctcaatgtag gagcctgtag cgccccggcc acccccatca taaaagcgac cagcataaat
     6301 gccgcgtaaa caccgttcat acggcgagcc atcgtcatta tccagatcat acgttccctt
     6361 tttagcgcgg cgagaataaa aaaaaccagc aggtataatc tgctggcggg tgattttaca
     6421 ccaatactca gtcacacatg atgcaggcaa gtcaactttc agaattgcgg tcatcccact
     6481 gcatcagttc ttcaagcatg gttaatcgtt gtgggacgct taaccaacgc aaccagcaca
     6541 acttcgctgt cgcggtaaaa tagtgctgat aaaactgacg catggggcac ctccttgctt
     6601 catcgttagg aattattgac ttaatatagg gaaaataaaa ttgctgtctt ttgcgcagga
     6661 gttccccttt tatgccatct gctcgtctgc aacaacagtt catccgcctg tggcaatgct
     6721 gcgagggtaa atcgcaggac acaacgctca acgaactggc agcgttattg agctgctcgc
     6781 gtcgtcatat gcgtaccctg ctcaacacca tgcaggatcg cggctggctg acgtgggaag
     6841 cggaagtcgg gcgcggtaaa cgctcgcgtc tgacattcct ctataccggg ctggcgcttc
     6901 agcaacagcg ggcggaagac ctgctggagc aggatcgtat cgaccaacta gtgcagttgg
     6961 ttggcgacaa agcgactgtg cggcaaatgc tggtttctca tctgggccgc agcttccgcc
     7021 aggggcggca catcctgcgc gtgctctact atcgtccgtt gcgtaatctg ctacctggca
     7081 gcgcattgcg ccgttccgaa acccatattg cccggcaaat cttcagttcg ctaacgcgca
     7141 taaatgagga aaatggggaa ctggaagcag acatcgccca ccactggcag caaatatcac
     7201 cgcttcactg gcgtttcttt ttgcgtccag gagtccattt tcatcatggt cgtgaactgg
     7261 aaatggacga cgtgatcgcc tctttaaaac gaatcaatac gctgccgctc tattcgcata
     7321 ttgcggacat tgtgtcgccg acgccctgga cgctggatat ccatctcacg caaccggacc
     7381 gctggttacc gttactgctg ggacaagttc cggcgatgat cctgccgcgc gaatgggaaa
     7441 ccctcagtaa ctttgccagc catcccatcg gcaccggtcc gtatgcggtg atccgcaaca
     7501 ccaccaatca actgaaaatt caggcattcg atgacttctt cggttaccgg gcattaatcg
     7561 atgaagttaa cgtctgggtt ctgccggaaa ttgccgacga gccagctgga ggtctgatgc
     7621 taaaaggtcc acagggtgag gaaaaagaga ttgaaagccg cctggaggaa ggttgctact
     7681 atttactgtt cgacagccgc acccatcgcg gggcgaatca gcaagtcagg gactgggtaa
     7741 gctatgtgct ttctccaact aatctggtct atttcgctga ggaacagtac cagcaactgt
     7801 ggttcccggc ttatggactg ctcccccgtt ggcaccatgc ccgcaccata aagagcgaaa
     7861 aaccggctgg cctggaaagc ctcatcctga ccttttatca ggatcacagt gagcatcggg
     7921 tgattgccgg gatcatgcag cagattctgg caagtcacca ggtcacgctg gaaatcaaag
     7981 agatcagcta cgatcagtgg catgaaggag agatcgagag cgatatctgg cttaacagcg
     8041 ccaactttac gctgcctctg gatttttcgc tgttcgcgca cttgtgcgag gtgccgctgc
     8101 tccaacactg tattccgatc gactggcaag ccgacgccgc ccgctggcgc aatggcgaaa
     8161 tgaacctggc gaactggtgc cagcaactgg tcgccagcaa agcaatggtg ccgcttatcc
     8221 accactggct gatcattcag gggcaacgca gtatgcgcgg cctgcgcatg aacaccctcg
     8281 gctggttcga ttttaaatca gcgtggtttg cgccaccgga tccatgattg ctggtagata
     8341 acaaaatcac tacactaacg ccgttctcaa cggggtgcca cgcaaacgcg tgcgctgaga
     8401 aaatacccgt cgaacctgat ccggataacg ccggcgaagg gatttgaggc tccttctcaa
     8461 gtcctttgcc actctttttt gaggtgcaaa gtgttaaaaa aatgtcttcc cctgctgttg
     8521 ctgtgcacag cgcccgtttt cgctaaaccc gttctgactg tttataccta cgattccttc
     8581 gccgccgact gggggcctgg tccaaagatc aaaaaagctt ttgaagccga ctgtaactgc
     8641 gaactgaaac tggtggcgct ggaagatggc gtttcgcttc tcaaccgtct acggatggaa
     8701 ggcaaaaata gtaaagccga tgtggtgctg gggctggata acaatctgtt agacgccgcc
     8761 agtaaaaccg gactatttgc caaaagcggt gtggcagcgg atgccgttaa cgttcccggc
     8821 ggctggaata atgacacttt cgtcccgttt gattacggct atttcgcctt cgtctatgac
     8881 aagaacaaac tgaaaaaccc gccgcaaagc ctgaaagaat tggttgagag cgatcagaac
     8941 tggcgggtga tttatcagga tctgcgcacc agtacgccgg ggctgggcct gctgttgtgg
     9001 atgcaaaaag tctatggcga tgacgcccca caagcctggc aaaaactggc gaagaaaacc
     9061 gtcacggtca ccaaaggctg gagcgaagcc tacggcctgt ttttaaaagg tgaaagcgat
     9121 ctggtactga gttacaccac ctctccggct tatcacattc tcgaagagaa aaaagataac
     9181 tacgccgccg cgaactttag cgaaggtcac tatctgcagg tggaagtcgc cgcccgcacc
     9241 gctgccagca agcagccgga gctggcgcaa aaattcctcc agtttatggt ttctccggct
     9301 tttcagaatg cgatcccaac cggtaactgg atgtatccgg tggcaaatgt cacgctgcct
     9361 gccgggtttg aacaattgac caaaccggcc accacgctgg agttcacacc agctgaagtg
     9421 gctgcacaac gtcaggcatg gattagcgaa tggcaacgcg ccgtcagccg ttaattcccg
     9481 gctggttaat tccaggtgta agcgccgcca cgctggtggt agcggttgcg ctcgcggcgt
     9541 ttctcgccct gtggtggaac gcgccgcagg gtaactgggt ggcagtctgg caggacagct
     9601 acctgtggca tgtggtgcgc ttctccttct ggcaggcgtt tctctcggcg ctactctctg
     9661 tcgtacccgc gatattcctc gcccgcgccc tctatcgcag gcgctttccg ggtcggctgg
     9721 cgctgttgcg tctgtgcgca atgaccttga tcctcccggt gctagttgct gttttcggca
     9781 ttcttagcgt ctatggtcgt cagggctggc tggcatcgct ctgccaatcg ctcggtctgg
     9841 agtggacctt ttcgccctac ggcctgcaag gcattttgct ggcgcacgta ttttttaatc
     9901 tgccgatggc gagccgctta ttactccagg cactggaaaa cattcccggc gaacaacgtc
     9961 agcttgccgc ccagcttggg atgcgcggct ggcatttttt ccgcttcgtc gaatggccgt
    10021 ggctacggcg acaaatcccg ccggttgccg cgcttatctt tatgctctgt ttcgccagct
    10081 tcgccaccgt gctatcgctg ggcggcggtc cgcaggcaac tactatcgag ctggctattt
    10141 atcaggcgct gagttacgac tacgatcctg cccgcgcggc gatgctggcg ctgctccaga
    10201 tggtgtgttg ccttgggctg gtgctgttga gtcagcgact aagtaaggcc attgcgcccg
    10261 gtaccacgct gctgcaaggc tggcgcgatc cggacgatcg tctgcatagc cgcatttgcg
    10321 acacggtgtt aatagtgctg gcgctgctgc tgttgctgcc accgttgctg gcggtgatcg
    10381 tcgatggggt aaatcgccag ttgccggaag tgctggcaca accggtgctg tggcaggcgc
    10441 tgtggacctc gttgcgtatt gcgctggcgg caggtgtatt gtgcgtagtg ctgaccatga
    10501 tgctgctatg gagcagtcgc gaactgcggg cgcggcagaa aatgctggcg ggtcaggcgc
    10561 tggagatgag cggcatgttg atcctcgcca tgccggggat tgtgctggct accggcttct
    10621 ttttactgct caacaacact atcggcctgc cacaatctgc tgacggcatt gtgattttca
    10681 ccaatgcgtt aatggcgatc ccttatgcgc tgaaagtact ggaaaacccg atgcgcgata
    10741 tcaccgcccg ctacagtatg ttgtgtcagt cgctgggcat tgaaggctgg tcgcgcttaa
    10801 aagtggtcga gctgcgcgcc ctgaaacgtc cactggcgca ggcgctggct tttgcctgcg
    10861 tgctgtcgat tggtgatttt ggcgtagtgg cgttgttcgg taacgatgat ttccgtaccc
    10921 tgccgtttta tctctaccag caaattggct cctatcgcag ccaggacggc gcggtcaccg
    10981 cgttaattct gctactactc tgttttctgc tgtttaccgt gattgaaaaa ctaccggggc
    11041 gaaatgttaa aactgactga tatcacctgg ctttatcacc atttgccgat gcgttttagc
    11101 ttaacggtag aacgcggcga gcaggtggca atcctcgggc caagcggcgc gggtaaaagt
    11161 accctgctga atttgattgc cggttttctg acgccagcca gcggttcgct gactatcgac
    11221 agcgttgatc acaccacgac gccgccgtca cgccgcccgg tatcgatgct gtttcaggag
    11281 aacaacctgt tcagccacct gacggtcgcg cagaacatcg ggctggggct taatccggga
    11341 ttgaagctga acgcggcgca gcaggagaaa atgcacgcta tcgcccgcca gatggggatt
    11401 gataatttaa tggcgcggtt accgggcgag ctttccggcg gtcagcgaca gcgcgtggcg
    11461 ttagcgcgtt gtctggtgcg cgaacagccg attttattgc tcgatgaacc gttctctgcg
    11521 ctcgatccgg cgttacgtca ggagatgctg acgctggtga gcacgagctg ccagcagcaa
    11581 aaaatgacgc tattgatggt gtcgcacagt gtggaagatg cggcgcggat cgccacacgc
    11641 tcggtggtgg tcgccgacgg gcgcatcgcc tggcagggta agaccaatga gttgttgagc
    11701 ggtaaggcga gtgcttcggc actattgggg attacgggtt agtatgcact gtcggatgcg
    11761 gcgtgaacgc cttatccgac ctacgggtag tgcgttttgt aggcctgata agacgcggtc
    11821 agcgtcgcat caggcaaccc gcacaagact atctcctaac ccccaaccac tttacgcaaa
    11881 atatcgatat acaccggcat caacgggtgg cgaattaaca ccaccagcgc caccacgccg
    11941 atggcagaaa ttaacggcgt cagccacaac aaacggccgc gcgacaaata atgacttaaa
    12001 cgatcggttg ctttaccgct gcgccataac cgccagcaca gccagccacc aacccacaaa
    12061 aataccgccg tcgccagcag caaccattta aactcgccgc tctgcattcc ggctggaata
    12121 tcgatcgccg cgcccgccag aatcccaggc aggaagtaaa acggtggcca cagcaggcag
    12181 ccgataatat tcggcgtaat gaattttgcc accggcagat ccagcattcc cgccaccatt
    12241 ggcaccagcg gacgcgtcgg gccaacaaag cgaccgacca gaatggtgaa catgctgtgt
    12301 tgatgcaacg catgttcagt tttatcgagt aatgctttgt ttttcttcag aaatgaccag
    12361 cgatgcaacg gctttttaaa acgccagccc agccagaagg agatccagtc gcccagcagg
    12421 caaccgacga tccctgccag ccaggcgtgc cagaaactta actcgccgct gccaatcagc
    12481 gctcccagcc ccgccatcag caccgtaccg ggtagaatca aaccgaccag cgccagcgac
    12541 tccagaaagg ccaccaacac caccgccatc aatgaataca cggtagattg ggtaataaag
    12601 tgttccagca atgcttgcat aatgtgcctg tcaaatggac gaagcaggga ttctgcaaac
    12661 cctatgctac tccgtcaagc cgtcaattgt ctgattcgtt accaattatg acaacttgac
    12721 ggctacatca ttcacttttt cttcacaacc ggctcggaac tcgctcgggc tggccccggt
    12781 gcatttttta aatacccgcg agaaatagag ttgatcgtca aaaccaacat tgcgaccgac
    12841 ggtggcgata ggcatccggg tggtgctcaa aagcagcttc gcctggctga tacgttggtc
    12901 ctcgcgccag cttaagacgc taatccctaa ctgctggcgg aaaagatgtg acagacgcga
    12961 cggcgacaag caaacatgct gtgcgacgct ggcgatatca aaattgctgt ctgccaggtg
    13021 atcgctgatg tactgacaag cctcgcgtac ccgattatcc atcggtggat ggagcgactc
    13081 gttaatcgct tccatgcgcc gcagtaacaa ttgctcaagc agatttatcg ccagcagctc
    13141 cgaatagcgc ccttcccctt gcccggcgtt aatgatttgc ccaaacaggt cgctgaaatg
    13201 cggctggtgc gcttcatccg ggcgaaagaa ccccgtattg gcaaatattg acggccagtt
    13261 aagccattca tgccagtagg cgcgcggacg aaagtaaacc cactggtgat accattcgcg
    13321 agcctccgga taacgaccgt agtgatgaat ctctcctggc gggaacagca aaatatcacc
    13381 cggtcggcaa acaaattctc gtccctgatt tttcaccacc ccctgaccgc gaatggtgag
    13441 attgagaata taacctttca ttcccagcgg tcggtcgata aaaaaatcga gataaccgtt
    13501 ggcctcaatc ggcgttaaac ccgccaccag atgggcatta aacgagtatc ccggcagcag
    13561 gggatcattt tgcgcttcag ccatactttt catactcccg ccattcagag aagaaaccaa
    13621 ttgtccatat tgcatcagac attgccgtca ctgcgtcttt tactggctct tctcgctaac
    13681 ccaaccggta accccgctta ttaaaagcat tctgtaacaa agcgggacca aagccatgac
    13741 aaaaacgcgt aacaaaagtg tctataatca cggcagaaaa gtccacattg gttatttgca
    13801 cggcgtcaca ctttgctatg ccatagcatt tttatccata agattagcgg atcctacctg
    13861 acgcttttta tcgcaactct ctactgtttc tccatacccg tttttttgga tggagtgaaa
    13921 cgatggcgat tgcaattggc ctcgattttg gcagtgattc tgtgcgagct ttggcggtgg
    13981 actgcgccag cggtgaagag atcgccacca gcgtagagtg gtatccccgt tggcaaaaag
    14041 ggcaattttg tgatgccccg aataaccagt tccgtcatca tccgcgtgac tacattgagt
    14101 caatggaagc ggcactgaaa accgtgcttg cagagcttag cgtcgaacag cgcgcagctg
    14161 tggtcgggat tggcgttgac agtaccggct cgacgcccgc accgattgat gccgacggta
    14221 acgtgctggc gctgcgcccg gagtttgccg aaaacccgaa cgcgatgttc gtattgtgga
    14281 aagaccacac tgcggttgaa gaagcggaag agattacccg tttgtgccac gcgccgggca
    14341 acgttgacta ctcccgctac attggcggta tttattccag cgaatggttc tgggcaaaaa
    14401 tcctgcatgt gactcgccag gacaccgccg tggcgcaatc tgccgcatcg tggattgagc
    14461 tgtgcgactg ggtgccagct ctgctttccg gtaccacccg cccgcaggat attcgtcgcg
    14521 gacgttgcag cgccgggcat aagtcgctat ggcacgaaag ctggggtggc ttgccgccag
    14581 ccagtttctt tgatgagctg gacccgatcc tcaatcgcca tttaccttcc ccgctgttca
    14641 ctgacacctg gactgccgat attccggtgg gcaccttatg cccggaatgg gcgcagcgtc
    14701 tcggcctgcc tgaaagcgtg gtgatttccg gcggcgcgtt tgactgccat atgggcgcag
    14761 ttggcgcagg cgcacagcct aacgcactgg taaaagttat cggtacttcc acctgcgaca
    14821 ttctgattgc cgacaaacag agcgttggcg agcgggcagt taaaggtatt tgcggtcagg
    14881 ttgatggcag cgtggtacct ggatttatcg gtctggaagc aggccaatcg gcgtttggtg
    14941 atatctacgc ctggtttggt cgcgtactcg gctggccgct ggaacagctt gccgcccagc
    15001 atccggaact gaaagcgcaa atcaacgcca gccagaaaca actgcttccg gcgctgaccg
    15061 aagcatgggc caaaaatccg tctctggatc acctgccggt ggtgctcgac tggtttaacg
    15121 gtcgtcgcac accgaacgct aaccaacgcc tgaaaggggt gattaccgat cttaacctcg
    15181 ctaccgacgc tccgctgctg ttcggcggtt tgattgctgc caccgccttt ggcgcacgcg
    15241 caatcatgga gtgctttacc gatcagggga tcgccgtcaa taacgtgatg gcgctgggcg
    15301 gcatcgcgcg gaaaaaccag gtcattatgc aggcctgctg cgacgtgctg aatcgcccgc
    15361 tgcaaattgt tgcctctgac cagtgctgtg cgctcggtgc ggcgattttt gctgccgtcg
    15421 ccgcgaaagt gcacgcagac atcccatcag cccagcaaaa aatggccagt gcggtagaga
    15481 aaaccctgca accgcgcagc gaacaggcac aacgctttga acagctttat cgccgctatc
    15541 agcaatgggc gatgagcgcc gaacaacact atcttccaac ttccgccccg gcacaggctg
    15601 cccaggccgt tgcgactcta taaggacacg ataatgacga tttttgataa ttatgaagtg
    15661 tggtttgtca ttggcagcca gcatctgtat ggcccggaaa ccctgcgtca ggtcacccaa
    15721 catgccgagc acgttgttaa tgcgctgaat acggaagcga aactgccctg caaactagtg
    15781 ttgaaaccgc tgggcaccac gccggatgaa atcaccgcta tttgccgcga cgcgaattac
    15841 gacgatcgtt gcgctggtct ggtggtgtgg ctgcacactt tctccccggc caaaatgtgg
    15901 atcaacggcc tgaccatgct caacaaaccg ttgctgcaat tccacaccca gtttaacgcg
    15961 gcgctgccgt gggacagtat cgatatggac tttatgaacc tgaaccagac tgcacatggc
    16021 ggtcgcgagt tcggcttcat tggcgcgcgt atgcgtcagc aacatgccgt ggttaccggt
    16081 cactggcagg ataaacaagc ccatgagcgt atcggctcct ggatgcgcca ggcggtctct
    16141 aaacaggata cccgtcatct gaaagtctgc cgttttggcg ataacatgcg tgaagtagcg
    16201 gtcaccgatg gtgataaagt tgccgcacag atcaagttcg gtttctccgt caatacctgg
    16261 gcggttggcg atctggtgca ggtggtgaac tccatcagcg acggcgatgt taacgcgctg
    16321 gtcgatgagt acgaaagctg ctacaccatg acgcctgcca cacaaatcca cggcgaaaaa
    16381 cgacagaacg tgctggaagc ggcgcgtatt gagctgggga tgaagcgttt cctggaacaa
    16441 ggtggcttcc acgcgttcac caccaccttt gaagatttgc acggtctgaa acagcttcca
    16501 ggtctggccg tacagcgtct gatgcagcag ggttacggct ttgcgggcga aggcgactgg
    16561 aaaaccgccg ccctgcttcg catcatgaag gtgatgtcaa ccggtctgca gggcggcacc
    16621 tcctttatgg aggactacac ctatcacttc gagaaaggta atgacctggt gctcggctcc
    16681 catatgctgg aagtctgccc gtcgattgcc gtagaagaga aaccgatcct cgacgttcag
    16741 catctcggta ttggtggtaa ggacgatcct gcccgactga tcttcaatac ccaaaccggt
    16801 ccagcgattg tcgccagcct gattgatctc ggcgatcgtt accgtctgct ggttaactgt
    16861 atcgacacgg tgaaaacacc gcactccctg ccgaaactgc cggtggcgaa tgcgctgtgg
    16921 aaagcgcaac cggatctgcc aactgcttcc gaagcgtgga tccttgctgg tggcgcgcac
    16981 cataccgtct tcagccatgc gctgaacctc aacgatatgc gccagttcgc cgagatgcac
    17041 gacattgaaa tcacggtgat tgataacgac acccgcctgc cagcgtttaa agacgcactg
    17101 cgctggaacg aagtgtatta cggatttcgt cgctaagtag ccacatcagg tatgtaacgc
    17161 ctgatgcgac gctgacgcgt cttatctggc ctacacgctg cgattttgta ggccggataa
    17221 gcaaagcgca tccggcattg ttttccccct cgcccctttg gggagagggc cggggtgagg
    17281 ggaaacgacc tctcaggagt caacatgtta gaagatctca aacgccaggt attagaagcc
    17341 aacctggcgc tgccaaaaca caacctggtc acgctcacat ggggcaacgt cagcgccgtt
    17401 gatcgcgagc gcggcgtctt tgtgatcaaa ccttccggcg tcgattacag cgtcatgacc
    17461 gctgacgata tggtcgtggt tagcatcgaa accggtgaag tggttgaagg tacgaaaaag
    17521 ccctcctccg acacgccaac tcaccggctg ctctatcagg cattcccctc cattggcggc
    17581 attgtgcata cgcactcgcg ccacgccacc atctgggcgc aggcgggtca gtcgattcca
    17641 gcaaccggca ccacccacgc cgactatttc tacggcacca ttccctgcac ccgcaaaatg
    17701 accgacgcag aaatcaacgg cgaatatgag tgggaaaccg gtaacgtcat cgtagaaacc
    17761 tttgaaaaac agggtatcga tgcagcgcaa atgcccggcg ttctggtcca ttcccacggc
    17821 ccgtttgcat ggggcaaaaa tgccgaagat gcggtgcata acgccatcgt gctggaagag
    17881 gtcgcttata tggggatatt ctgccgtcag ttagcgccgc agttaccgga tatgcagcaa
    17941 acgctgctgg ataaacacta tctgcgtaag catggcgcga aggcatatta cgggcagtaa
    18001 tgactgtata aaaccacagc caatcaaacg aaaccaggct atactcaagc ctggtttttt
    18061 gatggatttt cagcgtggcg caggcaggtt ttatcttaac ccgacactgg cgggacaccc
    18121 cgcaagggac agaagtctcc ttctggctgg cgacggacaa cgggccgttg caggttacgc
    18181 ttgcaccgca agagtccgtg gcgtttattc ccgccgatca ggttccccgc gctcagcata
    18241 ttttgcaggg tgaacaaggc tttcgcctga caccgctggc gttaaaggat tttcaccgcc
    18301 agccggtgta tggcctttac tgtcgcgccc atcgccaatt gatgaattac gaaaagcgcc
    18361 tgcgtgaagg tggcgttacc gtctacgagg ccgatgtgcg cccgccagaa cgctatctga
    18421 tggagcggtt tatcacctca ccggtgtggg tcgagggtga tatgcacaat ggcactatcg
    18481 ttaatgcccg tctgaaaccg catcccgact atcgtccgcc gctcaagtgg gtttctatag
    18541 atattgaaac cacccgccac ggtgagctgt actgcatcgg cctggaaggc tgcgggcagc
    18601 gcatcgttta tatgctgggg ccggagaatg gcgacgcctc ctcgcttgat ttcgaactgg
    18661 aatacgtcgc cagccgcccg cagttgctgg aaaaactcaa cgcctggttt gccaactacg
    18721 atcctgatgt gatcatcggt tggaacgtgg tgcagttcga tctgcgaatg ctgcaaaaac
    18781 atgccgagcg ttaccgtctt ccgctgcgtc ttgggcgcga taatagcgag ctggagtggc
    18841 gcgagcacgg ctttaaaaac ggcgtctttt ttgcccaggc taaaggtcgg ctaattatcg
    18901 acggtatcga ggcgctgaaa tccgcgttct ggaatttctc ttcattctcg ctggaaactg
    18961 tcgctcagga gctattaggc gaaggaaaat ctatcgataa cccgtgggat cgaatggacg
    19021 aaattgaccg ccgtttcgcc gaagataaac ctgcgctggc aacttataac ctgaaagatt
    19081 gcgagctggt gacgcagatc ttccacaaaa ctgaaatcat gccattttta ctcgaacgag
    19141 caacggtgaa cggcctgccg gtggatcggc acggcggttc ggtggcagcg tttggtcatc
    19201 tctattttcc gcgaatgcat cgcgctggtt atgtcgcgcc taatctaggc gaagtgccgc
    19261 cgcacgccag ccctggcggc tacgtgatgg attcacggcc agggctttat gattcagtgc
    19321 tggtgctgga ctataaaagc ctgtacccgt cgatcatccg cacctttctg attgatcccg
    19381 tcgggctggt tgaaggcatg gcgcagcctg atccagagca cagtaccgaa ggttttctcg
    19441 atgcctggtt ctcacgggag aaacattgcc tgccggagat tgtgactaac atctggcacg
    19501 ggcgcgatga agccaaacgt cagggtaata aaccactttc gcaggcgctg aagatcatta
    19561 tgaatgcctt ttatggcgtg cttggcacca ccgcctgccg ctttttcgat ccgcgcctgg
    19621 catcgtcgat caccatgcgt ggtcatcaga tcatgcggca aaccaaagca ttgattgaag
    19681 cgcagggcta cgacgttatc tacggagata ccgactcgac ttttgtctgg ctaaaaggtg
    19741 cacattcgga agaagaggcg gcgaaaatcg gtcgtgcact ggtgcagcac gttaacgcct
    19801 ggtgggcgga aacgctgcaa aaacaacggc tgaccagcgc attagaactg gagtatgaaa
    19861 ctcatttctg ccgttttctt atgccaacca ttcgcggagc tgataccggc agtaaaaagc
    19921 gctacgccgg gctgattcag gagggcgaca agcagcggat ggtgtttaaa gggctggaaa
    19981 ccgtgcgcac cgactggacg ccgctggccc agcagtttca gcaggagctg tacctgcgta
    20041 tcttccgcaa cgagccatat caggagtacg tccgccaaac catcgacaaa ctgatggcgg
    20101 gtgaactgga tgcgcggctg gtttaccgta aacgccttcg ccgtccgctg agcgaatatc
    20161 agcgtaatgt tccgcctcat gtacgcgccg ctcgccttgc cgatgaagaa aatcaaaagc
    20221 gcggtcgccc cttgcaatat cagaatcgcg gcaccattaa gtacgtatgg accaccaacg
    20281 gcccggagcc gctggactac caacgttcac cactggatta cgaacactat ctgacccgcc
    20341 agctacaacc cgtggcggag ggaatactcc cttttattga ggataatttt gctacactta
    20401 tgaccgggca acttgggcta ttttgagcaa aaaaaagagt tcgccagata ccattttgat
    20461 gcgtgacgaa tgctttgcca tccagtacca tagcgccctt tccattcctg gacctgaata
    20521 acgccactac ctcataagca cggtagcggg tggttattgc ctgcaattaa agatatagag
    20581 ccgaacacat atgcctttta cacttggtca acgctggatc agcgatacag aaagcgaatt
    20641 gggacttgga accgttgtcg cggtggatgc gcgaactgtc actttacttt tcccatctac
    20701 tggtgaaaac cgtctgtacg cacgcagtga ttcccccgtg acccgcgtga tgttcaaccc
    20761 tggtgatacc attaccagcc atgacggctg gcagatgcaa gtcgaagaag taaaagaaga
    20821 aaatggcttg ctgacctata tcggtactcg cctggatact gaagagtccg gcgtagccct
    20881 gcgtgaagtt ttccttgata gcaaactggt gttcagcaaa ccgcaggacc gtctgtttgc
    20941 cgggcagatt gaccgtatgg accgctttgc gctgcgttat cgcgcgcgta agtattccag
    21001 cgaacagttc cgtatgccgt acagcggcct gcgcggtcag cgtaccagcc tgatcccgca
    21061 tcagctcaac atcgctcatg atgttggtcg ccgccacgcg ccgcgcgttc tgctggctga
    21121 cgaagtgggt ttagggaaaa ccattgaagc cgggatgatc ctgcatcagc aactgctctc
    21181 tggcgctgct gaacgtgtgc tgattatcgt cccggaaacc ttacagcatc agtggctggt
    21241 agaaatgctg cgccgtttca acctgcgttt tgcgctgttt gatgatgagc gttatgccga
    21301 agctcagcac gatgcttaca acccgttcga caccgaacag ctggtgattt gctcgctgga
    21361 ttttgcccgt cgtagcaaac agcgcctgga acatctctgt gaagccgaat gggacctgct
    21421 ggtggtcgat gaagcgcatc acctggtgtg gagcgaagac gcgccgagcc gcgaatatca
    21481 ggctattgaa caactggcag agcacgtgcc gggcgttctg ctgctgaccg caaccccgga
    21541 acagttggga atggaaagcc acttcgcccg tctgcgtctg ctggacccga accgtttcca
    21601 cgattttgcg cagttcgttg aagagcagaa aaattatcgt ccggttgcgg acgccgttgc
    21661 catgctgctg gcaggtaaca aactgagcaa tgacgaactg aacatgcttg gcgagatgat
    21721 cggcgagcag gatatcgagc cgctgttgca agcagcaaac agcgacagcg aagatgccca
    21781 gagcgcccgt caggaactgg tttcgatgct gatggatcgc cacggcacca gccgcgtgct
    21841 gttccgtaac acccgtaacg gtgtgaaagg attcccgaaa cgcgagctgc acaccattaa
    21901 gctgccgcta ccgacgcagt atcagacggc tattaaagtc tccggcatta tgggcgcacg
    21961 taaaagtgcg gaagatcgtg ctcgcgatat gctctacccg gagcgtattt atcaggaatt
    22021 tgaaggtgat aacgccacct ggtggaactt cgatccgcgc gttgagtggc tgatgggcta
    22081 cctgaccagc catcgctctc agaaagtgct ggtgatctgc gccaaagctg ccactgcgct
    22141 gcaactggag caggtactgc gcgaacgtga aggtattcgc gctgcggtgt tccacgaagg
    22201 tatgtcgatt atcgaacgtg accgcgctgc cgcctggttt gccgaagaag acaccggcgc
    22261 acaggtactg ctgtgctcag aaatcggttc tgaaggacgt aacttccagt tcgccagcca
    22321 catggtaatg tttgacctgc cattcaaccc ggatctgctg gagcagcgta ttggtcgtct
    22381 ggatcgtatc ggtcaggcgc acgatattca gatccatgtg ccttatctgg aaaaaactgc
    22441 tcagtcggtg ctggtgcgct ggtatcacga aggtctggat gcattcgagc acacctgccc
    22501 gaccggacgc actatttacg atagcgtcta caacgatctg attaactatc tggcttcacc
    22561 ggatcaaacc gaaggctttg acgatctgat caaaaactgc cgcgagcaac atgaagcgct
    22621 gaaagcacaa ctggaacagg ggcgtgatcg cctgctggaa atccactcca acggtggcga
    22681 aaaagcccag gcactggcag aaagcattga agagcaggat gacgatacca acctgatcgc
    22741 cttcgccatg aacctgttcg atattatcgg tatcaatcag gacgatcgcg gcgacaacat
    22801 gatcgtgctg accccgtccg atcatatgct ggtgccggac ttccctggcc tgtcggaaga
    22861 tggcatcacc atcacctttg atcgtgaagt ggcgctggcg cgtgaagatg ctcagtttat
    22921 tacctgggaa catccgctga tccgcaacgg tctggatctg attctttctg gcgataccgg
    22981 tagcagcacg atttcactgt taaaaaacaa agcgttgccg gtaggtacgc tgttggtgga
    23041 actgatttac gtggtcgaag cccaggcgcc gaagcagttg cagctcaacc gcttcctgcc
    23101 accgacgccg gtacgtatgc tgctggataa aaacggcaac aacctggcgg cccaggtgga
    23161 gtttgaaacc tttaaccgcc agcttaacgc ggttaaccgt cacaccggca gcaaactggt
    23221 taacgccgtg cagcaggatg ttcacgctat ccttcagctg ggtgaagcgc agatcgagaa
    23281 atctgcccgt gcattgattg atgccgcacg taacgaagcc gacgaaaaac tgtctgccga
    23341 gctgtctcgt ctggaagcgc tgcgcgcagt gaacccgaac attcgtgacg acgaactgac
    23401 cgccattgag agcaaccgtc agcaggtaat ggaaagcctg gatcaggcag gctggcgtct
    23461 ggatgccctg cgtttgatcg ttgtaacgca tcagtaacgg agccgaaaat ggggatggaa
    23521 aactacaatc caccgcagga accctggttg gttatcctgt atcaggatga ccacattatg
    23581 gtggttaaca agccgagcgg tttgttgtct gtgccgggtc gtctggaaga gcacaaagat
    23641 agcgtgatga cgcgcattca gcgtgattat ccgcaggcag aatcggtgca tcgtctggat
    23701 atggctacca gcggcgtgat tgtagtggcg ctgaccaaag ccgcggagcg ggagttaaaa
    23761 cgccagttcc gcgagcgcga gccgaaaaag cagtatgtgg cccgcgtctg ggggcatcca
    23821 tcccccgcag aaggtctggt ggatctgccg ctgatttgcg actggccaaa ccgcccgaaa
    23881 cagaaagtct gttacgaaac gggtaaacct gcgcagacgg aatatgaagt ggtggagtat
    23941 gtggcggata acacggcaag agtggtgtta aaaccgatta ccgggcgttc gcatcaattg
    24001 cgtgtgcata tgctggcgct gggtcatccg attctcggcg atcgttttta tgcatcacca
    24061 gaagcgagag cgatggcacc acgtttgttg ctacatgcag agatgttgac gattacccat
    24121 ccggcgtatg gcaatagtat gacgtttaaa gcgccagcgg atttttaatc gtcacgctgg
    24181 cgcagttcgg taggcggata aggcgttcac gccgcatccg gcaattgtgc gccgattgcc
    24241 tgatgcgacg cgggccgcgt cttatcaggc ctacagttta aaacttattt aaaccctttc
    24301 tgctgcttta tcagctcata tgcctgctga atttcctgcg ctttctgctt cgccatctcc
    24361 atcatctcag gcggcaaacc tttcgccacc agcttatcgg gatggtgttc actcatcagc
    24421 ttacggtagg cacgtttgat ggtggtcgca tcatccgtcg gcttcacgcc cagcacatta
    24481 caggcatctt ccagcgtcgg gccacgctgc gcttgctgcc agttaccacc gccagattgc
    24541 tgctgataac cgccgccaaa ctgtgcaccg ccctgcatca tgcgcaaaaa ctggtcaaac
    24601 tgggcgcggg agatccctaa ttcttcagca atgacataca gcaccgcccg ttcattcggg
    24661 tgcagtgaac catcagcaaa cgccgcctga atctggatct ccagaaacat acgaattaag
    24721 tcaaaacgac caaagcagac actgcgaaac tggcgcatct tttcgcgcag cgggtaatta
    24781 tctgattttc ccactcggaa cgcgttttgc gccgcagtac gggaagcgcc atgaaggttc
    24841 attcggtcca tcaactggct ggcgatatga atatcagcct ccgtgacgcg acctttggat
    24901 ttggttaaat gccccatcac ttcaaaagtg gtggcaaaaa acagcgcctg acgctcacgc
    24961 tggttggcga accacgccat tttacggcta cgggctttat caaacatatg gccaattaac
    25021 aggcctaaca ctacgcccca aaagccgccg cccatcagta aggccacggc cacgccaatg
    25081 atttttcccc aatactgcat atattcccca aatcgacaca cggatatcag ggctatctcc
    25141 cacaatataa aggtgctttt accgttttcc ggctgcggtc aattgtggga catcgcctat
    25201 aatttgcatt atcatacctg tcattcaatg ccgtgcctaa caccactgac gctattcgga
    25261 caggattaac actagcgtag agatgacgag tacgttagtc tctgaccgtt tgtcacgcgc
    25321 aacgttaccg atgatggaac aataaaatca acgtatgaaa aaacgtatcc ccactctcct
    25381 ggccaccatg attgccaccg ccctttatag tcaacaggga ctggcagccg acctcgcctc
    25441 acagtgcatg ttgggcgtgc caagctatga ccgtcctctg gtacagggcg ataccaatga
    25501 cttacccgtg actatcaatg ctgaccacgc gaaaggggac tacccggatg acgccgtgtt
    25561 tactggcagc gtggatatca tgcagggtaa cagccgtctg caggccgacg aagtgcagct
    25621 ccatcaaaaa gaggcaccag gacaaccgga gccggtacgt accgttgatg cgctcggtaa
    25681 tgtccattac gacgataacc aggtgatcct caaagggccg aaaggctggg cgaatctgaa
    25741 caccaaagat accaacgtct gggaaggtga ttaccagatg gtgggtcgcc agggtcgcgg
    25801 taaagcggac ctgatgaaac aacgtggcga aaaccgctat accattctgg ataacggtag
    25861 ctttacctcc tgtctgccgg gttctgacac ctggagcgtg gtaggtagcg aaattattca
    25921 tgaccgcgaa gaacaagttg cggagatctg gaacgcccgc tttaaggtgg gtccggtacc
    25981 gatcttttat agcccctatt tgcagttgcc ggtgggtgac aaacgtcgct ctggtttctt
    26041 gatcccgaac gccaagtaca ccaccaccaa ctactttgag ttctacctgc catattactg
    26101 gaacatcgcg ccaaatatgg atgccaccat cacgccgcat tatatgcatc gtcgtggcaa
    26161 catcatgtgg gagaacgaat tccgctacct ctcccaggcg ggcgctggct tgatggaact
    26221 ggactatctg ccttcagata aagtctatga agatgaacac ccgaacgatg acagttcacg
    26281 tcgttggtta ttctactgga accactccgg ggtcatggat caggtgtggc gtttcaacgt
    26341 cgactacacc aaggtcagcg atcctagcta cttcaatgat ttcgataaca agtacggttc
    26401 cagtactgac ggctacgcaa cgcaaaaatt cagcgttggc tatgcggtgc aaaacttcaa
    26461 tgccaccgtt tcaaccaagc agttccaggt tttcagcgaa cagaacacca gtagctactc
    26521 ggcagagccg cagttagacg ttaattacta ccagaatgat gttggtccat ttgatacgcg
    26581 tatttacggc caggcagtgc actttgttaa caccagagac gacatgcctg aagcaacccg
    26641 tgttcacctg gaaccgacca tcaatttgcc gctctctaat aactggggca gcatcaatac
    26701 cgaagcgaag ttgctggcaa cccattatca gcaaaccaat cttgactggt ataactccag
    26761 aaacacgacc aagctggacg aatccgttaa ccgcgtaatg ccgcaattca aagttgacgg
    26821 caaaatggtc tttgaacgcg atatggaaat gctggctccg ggttataccc aaacgctgga
    26881 accgcgcgcg cagtatttgt acgtgccgta tcgcgatcag agcgacatct ataactacga
    26941 ctcgtctctg ctgcaatctg actactctgg cctgttccgg gaccggactt acggcggtct
    27001 tgaccgtatt gcctccgcta accaggtgac gaccggtgtc acatctcgca tatatgatga
    27061 tgctgccgtt gaacgtttta atatttccgt tggtcaaatc tactatttca cggagtctcg
    27121 cactggcgat gacaacataa catgggagaa tgacgacaaa acgggttcac tggtgtgggc
    27181 aggcgatact tactggcgta tctccgagcg ttggggattg cgtggcggga ttcagtacga
    27241 tacacgtctg gataacgtag cgaccagtaa ctccagcatt gaataccgtc gggatgaaga
    27301 ccgtctggta cagctgaatt accgttacgc cagcccggaa tatattcagg ctacgctgcc
    27361 taagtactat tccactgctg agcaatataa gaatggtatt tcgcaggtag gtgctgtcgc
    27421 cagctggcca attgccgatc gttggtccat tgttggggcc tactactacg acaccaatgc
    27481 gaacaagcaa gccgactcta tgttaggtgt gcaatacagc tcctgctgct atgcaattcg
    27541 cgtcggttac gagcggaagc tgaacggttg ggataacgat aaacaacatg cggtatatga
    27601 caacgcaatc ggctttaaca tcgaacttcg cggcctgagc tccaactacg gtctgggtac
    27661 gcaagagatg ctgcgttcga acattctgcc gtatcaaaac actttgtgat ttgttgattt
    27721 accacgtaat ccgcagtgcg gttaattgaa atggaaaaag tatgaagaac tggaaaacgc
    27781 tgcttctcgg tatcgccatg atcgcgaata ccagtttcgc tgccccccag gtagtcgata
    27841 aagtcgcagc cgtcgtcaat aacggcgtcg tgctggaaag cgacgttgat ggattaatgc
    27901 agtcggtaaa actgaacgct gctcaggcaa ggcagcaact tcctgatgac gcgacgctgc
    27961 gccaccaaat catggaacgt ttgatcatgg atcaaatcat cctgcagatg gggcagaaaa
    28021 tgggagtgaa aatctccgat gagcagctgg atcaggcgat tgctaacatc gcgaaacaga
    28081 acaacatgac gctggatcag atgcgcagcc gtctggctta cgatggactg aactacaaca
    28141 cctatcgtaa ccagatccgc aaagagatga ttatctctga agtgcgtaac aacgaggtgc
    28201 gtcgtcgcat caccatcctg ccgcaggaag tcgaatccct ggcgcagcag gtgggtaacc
    28261 aaaacgacgc cagcactgag ctgaacctga gccacatcct gatcccgctg ccggaaaacc
    28321 cgacctctga tcaggtgaac gaagcggaaa gccaggcgcg cgccattgtc gatcaggcgc
    28381 gtaacggcgc tgatttcggt aagctggcga ttgctcattc tgccgaccag caggcgctga
    28441 acggcggcca gatgggctgg ggccgtattc aggagttgcc cgggatcttc gcccaggcat
    28501 taagcaccgc gaagaaaggc gacattgttg gcccgattcg ttccggcgtt ggcttccata
    28561 ttctgaaagt taacgacctg cgcggcgaaa gcaaaaatat ctcggtgacc gaagttcatg
    28621 ctcgccatat tctgctgaaa ccgtcgccga tcatgactga cgaacaggcc cgtgtgaaac
    28681 tggaacagat tgctgctgat atcaagagtg gtaaaacgac ttttgctgcc gcagcgaaag
    28741 agttctctca ggatccaggc tctgctaacc agggcggtga tctcggctgg gctacaccag
    28801 atattttcga tccggccttc cgtgacgccc tgactcgcct gaacaaaggt caaatgagtg
    28861 caccggttca ctcttcattc ggctggcatt taatcgaact gctggatacc cgtaatgtcg
    28921 ataaaaccga cgctgcgcag aaagatcgtg cataccgcat gctgatgaac cgtaagttct
    28981 cggaagaagc agcaagctgg atgcaggaac aacgtgccag cgcctacgtt aaaatcctga
    29041 gcaactaatg gttaaaaccc aacgtgttgt gatcactccc ggcgagcccg ccgggattgg
    29101 cccggactta gttgtccagc ttgcacagcg tgagtggccg gtcgaactgg ttgtttgtgc
    29161 cgatgccact ctccttaccg accgggcagc gatgctcggt ttgccgctca ccctccgccc
    29221 ttattccccc aactcccctg cacaaccgca aactacgggc acattaacgc tacttcctgt
    29281 cgcgctacgt gaatctgtca ctgcggggca gttagcgatt gaaaatggac attacgtggt
    29341 ggagacgctg gcgcgagcgt gtgatggctg tctgaacggt gaatttgctg cgctgatcac
    29401 aggccccgtg cataaaggcg tcattaacga cgcaggcatt ccgtttaccg gtcataccga
    29461 gtttttcgaa gagcgttcgc aggcgaaaaa agtggtgatg atgctggcga ccgaagaact
    29521 tcgcgtggcg ctggcaacga cgcatttacc gctgcgcgat atcgcagatg ctatcacccc
    29581 tgcacttttg cacgaagtga ttgctatttt gcatcacgat ttgcgtacaa aatttggtat
    29641 tgccgaaccg cgcattctgg tctgcgggct gaatccgcac gcgggcgaag gcggtcatat
    29701 gggtacggaa gagatagaca ccattattcc ggtgctcgac gagctgcggg cgcaggggat
    29761 gaaactcaac gggccgctgc ctgccgatac cctgtttcag ccgaaatatc tcgataacgc
    29821 cgacgccgtg ctggcgatgt accacgatca gggtcttccc gtgctaaaat accagggctt
    29881 cgggcgcggt gtgaacatta cgctgggcct gccctttatt cgcacatcag tggaccacgg
    29941 caccgcgctt gaactggcgg gacgtggcga agccgatgtc ggcagtttta ttacggcgct
    30001 taatctcgcc atcaaaatga ttgttaacac ccaatgaata atcgagtcca ccagggccac
    30061 ttagcccgta aacgcttcgg gcaaaacttt ctcaacgatc agttcgtgat cgacagcatt
    30121 gtttctgcca ttaacccgca aaagggccag gcaatggtcg aaatcggccc cggtctggcg
    30181 gcattgaccg agccggtcgg cgaacgtctg gaccagctga cggtcatcga acttgaccgc
    30241 gatctggcgg cacgtctgca aacgcatcca ttcttaggcc cgaaactgac gatttatcag
    30301 caggatgcga tgacctttaa ctttggcgaa ctggccgaga aaatgggtca gccgctgcgt
    30361 gttttcggca acctgcctta taacatctcc acgccgttga tgttccatct gtttagctat
    30421 actgatgcca ttgccgacat gcactttatg ttgcaaaaag aggtggtgaa tcgtctggtt
    30481 gcaggaccga acagcaaagc gtatggtcga ttaagcgtca tggcgcaata ctattgcaac
    30541 gtgatcccgg tactggaagt tccgccgtca gcctttacac caccacccaa agtggattcc
    30601 gccgtcgtgc gcctggttcc tcatgcaacg atgcctcacc cggttaaaga tgttcgtgtg
    30661 ttgagccgca tcaccaccga agcctttaac cagcgtcgta aaaccattcg taacagcctc
    30721 ggcaacctgt ttagcgtcga ggtgttaacg ggaatgggga tcgacccggc gatgcgagcg
    30781 gaaaatatct ctgtcgcgca atattgccag atggcgaact atctggcgga gaacgcgcct
    30841 ttgcaggaga gttaacgatg atcaattcgc cccgagtgtg tattcaggtt caaagcgtct
    30901 acattgaggc acaatcttca cctgataatg aacgttacgt ttttgcttat accgtaacca
    30961 tacgcaatct ggggcgagcg ccagtgcagt tgttggggcg ttactggctg atcaccaatg
    31021 gcaatggccg tgaaaccgaa gtccagggcg aaggagtggt tggcgtccag ccacttatcg
    31081 cgcctggcga agagtaccag tacaccagcg gtgcaatcat tgaaaccccg ctgggcacca
    31141 tgcagggtca ctacgaaatg atcgatgaaa atggcgtccc tttcagcatc gacattcccg
    31201 tattccgact cgccgttccc acactcattc attaaaagaa tatggcgaca taccttattg
    31261 gcgacgttca tggttgttac gatgaactgt tcgcattgct gcataaagta gaatttaccc
    31321 ctgggaaaga taccctctgg ctgacgggcg atctggtcgc gcgcgggccg ggttcgctgg
    31381 atgttctgcg ctatgtgaaa tccttaggcg acagcgtacg tctggtgctg ggcaatcacg
    31441 atctgcatct gctggcggta tttgccggga tcagccgcaa taaaccgaaa gatcgcctga
    31501 caccgctgct ggaagcgccg gatgccgacg agctgcttaa ctggctgcgt cgccagcctt
    31561 tgctgcaaat cgacgaagag aaaaagttgg tgatggccca cgcagggatc acgccgcagt
    31621 gggatctaca gaccgccaaa gagtgcgcgc gcgatgtaga agcggtgcta tcgagtgact
    31681 cctatccctt ctttctcgac gctatgtacg gcgatatgcc aaataactgg tcaccggaat
    31741 tgcgggggct gggaagactg cgttttatca ccaacgcctt tacccgtatg cgtttttgct
    31801 ttccaaacgg tcaactggat atgtacagca aagaatcgcc ggaagaagcc cctgccccac
    31861 tgaaaccgtg gtttgcgatt cctggccctg tcgctgaaga atacagcatc gcctttggtc
    31921 actgggcatc gctggaaggc aaaggtacgc cggaaggtat ttacgcgctg gataccggct
    31981 gctgctgggg tgggtcatta acctgcctgc gctgggaaga taagcagtat tttgtccagc
    32041 cgtcgaaccg gcataaggat ttgggtgagg gagaggcggt cgcgtcttaa cgaattacca
    32101 ttcagggagg aataatggct gactattttg aaatacgtgc tgattacaat cagcacacca
    32161 tcacgattta tcaggcttac aatgatgcaa tcgctgacgt cgccgtacgt gacgggcgat
    32221 ttggcgcacc tttttcattc aaccgcatga cctggattaa accctctttt atgtggatga
    32281 tggagcgtag caactgggga ctgaaaaaag atcaacaaca cattctcgca atccgaatca
    32341 aacgcacctt ctttgataca ttgctggaac aagcggtgct aaccacgccg gaagcccatg
    32401 tgtatccaca tgcaggaatt tgggaaacgt tatttgcaca agcaaatgtg tatgttcagt
    32461 gggacccaga acgcagcata aatggtaaaa aactggagca ccgatcatta cagcttggta
    32521 tttctcgtaa cttgatcagt caatttaacg aagacgcgat tgttgctatt gatgatctga
    32581 cgccgctggt gcgaaagtgt cataaccttt tgataaacgg caaaacaaca caggcgaaat
    32641 ccttcctgcc gccagaaaaa atttacccag ttagtgcagc agcgagaaag gctttgggaa
    32701 tgaaaaattc ctgagacaaa tacgcaccta ttaagcggaa aatatctaag tgcgacaaat
    32761 caacaccggg cttaatacca gagaaattat tctggaaatg attgacgaag cagatgcccc
    32821 cccgtttgct gagcagcgga cctggcatcg caggcgaatc gcttttctat gtgattgcat
    32881 cagcgttgct gtcgactacg gcttcttctg ttctgtctct cagcagtcaa taatgaacat
    32941 aaccaagatg gctgaagatg tatggataac agaaagtaaa acgacccaac tcattgatat
    33001 ttggcaacaa cttaaacagc tttatccgcc agaaactatt ccctacgccc agaagttacc
    33061 cactaaggaa ttgcttgaac tgctactttc tagtcctgac gatcttcgaa taattgaaat
    33121 tcctgacatc atcggtgatg tttttgatga ccaaacctgg attcgattat tcaaaaaaca
    33181 ctttgctgat gttattcagc tacgatgtga tgtgtaacaa tccaaaatat atcgccagac
    33241 gatagaccaa aaagaaagta tttagcagat actcaacatt ctgacttctg tacagaaata
    33301 gcagagttta aaaacttaaa acttccggta ctcatcagag aaacatccag tttcttctgc
    33361 aaattcattt aatgccgaga tggcatcctc gtttacttca ttccactgta gagtggcctg
    33421 aaggtttaga cctacgcaat gagcgcgact cacaaaatcg cgctcactgg taaaatcaaa
    33481 cgtcttatcg ttcaatttgt agtcctggca aaaaattacc gccgctccag aatctcaaag
    33541 caatagctgt gagagttctg cgcatcagca tcgtggaatt cgctgaatac cgattcccag
    33601 tcatccggct cgtaatccgg gaaatgggtg tcgccttcca cttctgcgtc gatatgcgtc
    33661 agatacagtt tctgcgcttt tggcaggaac tgctcataaa cgcgaccgcc gccaatcacc
    33721 atgatttctg gtacgtcacc acacgccgcg atggcttcat ccaccgactt cacccacgtt
    33781 acgcgatcgt ccgtacccgg ttgactgctg aggataatat ttttgcgtcc tggcaacgga
    33841 cgaccgattg attcccaggt atggcggccc ataatcacgg gtttatttaa ggtgttgcgt
    33901 ttaaaccagg cgagatcggc aggcaggttc cacggcatgg cgttttccat gccgataacg
    33961 cgatctaccg ctaacgccgc aatcagactg atcattgaga tttcccgata aaaaaaattg
    34021 tcgccactat acgtaaagcg taaaccgtcg tcgactggtg cgaggatgat gttgaggaaa
    34081 attttatatt ctgctggcga gtccacgctc tctccctgga ctcgccgcat tacaatgaaa
    34141 caaaaacaaa cagttagctg taaagtgtga tttacgtcac tctttattag gatgagggtt
    34201 tcgtttccgg ttcatccgcc atgttgccgg tatgtttacc ttcttcggtt ccctgccagc
    34261 catgtcgttg aattaatgac agatgttcgc ggtcctcggt aatgatctca cttaacatcg
    34321 cgctggtgcg tttatagacc gccgcgcggg ctttggtgtc gttctcaacc attgccatct
    34381 cttccaccat ctgaatatta aagcggcgga acacatcggc acgttctcgc gcttcatacg
    34441 gccccagacc taaactttcc agtgccagac gcccggtttt cagcgcacct tcgaaggttt
    34501 cacgctccgg cttttcaacg cctgcctgac gcaaacgaat gtagtggtcg acatcgcggg
    34561 cgcgggcaat aatctgcaaa tgcgggaaat gttctttcac catctccgtc agttgcaggt
    34621 tggtttgcgg atcatcaata gcattaatca gcacttccgc tttcgccgct ccggcagatt
    34681 ccagtaaatc catccgcgtt gcatcgccat aaaacacttt cataccaaat ttacgcagcg
    34741 tttcgatatg gtccggatcg tgatcgagca ccaccatttt caccccgctg gagagcagta
    34801 aacgtccggt aatctgccca aaacgaccga atccggcgat aatcacgcgc ggctgttctt
    34861 cgtcgatctc atcggcttca cgcgcttcct cagtagaaga ttgctcaagg cgattgagga
    34921 tcaccagcag aatcggcgtt gctgccatcg acagcgccac cgccagggtc agcgatttcg
    34981 cccactccgg ctccagcata ttcgccatct gcgccgcgcc aaataccaca aaggcaaact
    35041 cactgccctg ccctaacaac accgcaaacc aacgacgctg tttatttggc acttgcaacg
    35101 gtcgggcaat cagccacagc atggcgattt tgatgatgag gaaaccgagc agcaaaatga
    35161 caatgcgcaa tgggttttca agcagcgtgc caaagtctat cgacatgcca acaccgatga
    35221 aaaacagccc caacagcaaa cctttaaatg gttcgatatc gctctccagc gcatgacggt
    35281 attccgagct tgccagcagt acgcccgcca gaaacgcgcc catcgccatc gacaagccga
    35341 cctcttccag cagcaaacca aagccaaaca cgaggaataa cgccacggca ctaaacactt
    35401 cccgcaagcc agagcgggct acaaaacgca gcgccggacg cgtgacatag cgccccagca
    35461 ataccaccag caccagcgca cccgccactt ttaacgccga gagagcaaat gcgcccatcg
    35521 tcgtcgaggc actgctcgtt gccagtagcg gaatcatcgc caccagcggg atcgccgcga
    35581 tatcctggaa cagcagcacc gcaaaggcac tgcgacccat ttgcgtcacc atcagattgc
    35641 gttcattcat cgcctgcatg gcaatcgccg tagaggagag cgctagcgtc atgccgatca
    35701 attccgcgac ctgccagcgc agcccaagta acatgcagaa cagccccagc aggccgccgc
    35761 aaatcaccat ctgcaatgcg ccaccgccga acactgccgc acgcagcttc cacagccttt
    35821 gtggatcgag ttcgaggccg ataataaaca gcatcagcac caccccaatc tcggcaaagt
    35881 gcagaataga ttcggcatcg gtcaccagtc gcagccccca cgggccaata atgcagccgg
    35941 cgatcaggta gccaagtacc gatcccagac caagacgtac cgcaatgggt acaatcagcg
    36001 ctgccgaacc gagataaatc agcgcctgaa tcagcgtatg gctatccatg atgggcctcc
    36061 tgccattcca gcagacgttg cttatagtga cgcgcctgcc cttcgagggt ttcgtcgtca
    36121 caaataaagg tgcagtgcat ggcaaacggt ggcagccagt tcagtccgca gtagattgcc
    36181 gtcgcctgta gcggctgcga cagcacatca aagcccggat gcgcaccaat ttcaaaatgg
    36241 ctttccccgc cgccggtcgt caccgcccac agcaaatgtt tgccatgcag cgccgtgccg
    36301 ccatgaccgt aagcccagcc gtgggagaaa actttatcga tccaaagttt gaggagcgga
    36361 ggaatgctgt accactgcat cgggtgctgc cagacgatca gatcggcgcg agacagcgcc
    36421 tcctgctcgg cggcaatatc gatattgaag tcaggataga gttgataaag agagcgaatt
    36481 tcgacgcctt ccagcgtcct tgcctgttca agcatccgtt tattcgcatg ggaatgatgc
    36541 ggatacggat gcgcataaat tataagaatc ataggtagcc cgcgtgatta cgcctttgtt
    36601 ttcccacaaa gtgtagtcat atattcacgc ggcttatagt caataatatt gattcagaaa
    36661 agagagaaat ctgaacaatt taccccattc tctgcccgcg gatcgtcata ttgctggttt
    36721 gcgccagtga catccctcga gtctccgggg caaacgctac ggaaatcaac aagccaaaca
    36781 gcgagatacc cgcccccatt agcatcgtgt tactgatacc gtaattattg ataaagatcg
    36841 gcagtgccca ggtcgaaaca atggtgccaa tacggcttaa ggacataatc acgcccacgg
    36901 cagaggcgcg gatatctgtc gggaagagtt cattaggata gagccactgc aaattacccg
    36961 gcccgccaga gaaaaaggca tacaccgcaa aggccatcac taccagccag atccccatat
    37021 ccgggatcag ccccaaaacc gccagcgcca gcgtcatcat ggcaaagctg ccaatcaaca
    37081 atggacgccg tccggcagtg tttaaccaca gcatcggcgg aatacagccg agcataaaga
    37141 acaggctaat caccacgttc cccagcgccg cgtttttgcc aacacccaat cccaacaaac
    37201 caacgatttg cgggccaaag gtgtaaatgg cgaacattgg gatcacctgg caggtccaga
    37261 tggcggcaac aaacagaaca aaaggaaaat ggcggcgatt aaacagatcg cgaaaacggg
    37321 tttgctgcgg ctgctcttca tcgaaagcca ccggttcgcc aaacagtttt atcatcatct
    37381 cttcgcactc ttttactcgc cctttgcgta ataaccagcg gggagattca ggcagttcga
    37441 atcgaccaat caaaatcaac aaacagggga tcgccgcgct acccagcatc cagcgccagc
    37501 cgccttccac atcataaagc cagtagccga ccagatcggc gcaggtcgcg ccgacatacc
    37561 acatggcggc gataaagctg atggaaaacg cccgctgacg ggtactggag aactcggtga
    37621 tcattgaggt ggcgatggga taatctgcac cgatgacaat gccgataagt acccgcatca
    37681 ccaacagttc gacgggggat gaaacaaaca tcgtcgccac cgatatcacg ccgatggcga
    37741 tgatatcaat gaggaacatt ttgcgccgtc cgactttatc ggaaatatag ccaaacagcg
    37801 atgtgccaac gaacagcccg gcgagcgttc ccgcgcccaa caagccaatc cagtcagcgt
    37861 ccagttttag cgccggagtc agttgctcca gcgccacgcc aatcattacc agtacataac
    37921 catccagaaa cggaccaccg cttccccaca gcaaaatgcg gcggtgaatt gaggagaatt
    37981 tgagatcgtc aaagtttctg gacggttgca tgggttattc ctgttttttt gcaatgagag
    38041 tcagtacggc gttacgtatc aaaccaacat cagccgtaac ggaactccac accaaaggtg
    38101 ccgcgcgggt attcccactg ttccagcgcc gaccccagcc ccagaatgcg acaggtgccg
    38161 cactccagac atccggcgta atcgaagcgc acactgccgt catcctgctt cttgtacaga
    38221 cctgcggggc acgctttcac cagcagctcc agggcctgtt tatcggcatc ggccttcaca
    38281 acaatgtgcg gatgctcttc atcgacattg aatttattga cgcccagttt gacgtccaca
    38341 ttgacgggag aagtcataaa acggtcactc ctttcatgcc atccttgatc agattgatga
    38401 agcccacttt cttgccgtgg cggaggattt tcttgcgcat cagttccggc gcactgccgt
    38461 caatggtgaa caggtcacgc gccacgccca ccgccagttc cgggtagccg ctaaacatgc
    38521 gtgggttatc aaggaaggcc ggtagtttct ggtacatacg catatcgcgc agcgggccac
    38581 tctcaagatg ctgacgatat tccgcgagtt tttgcttact gaaatcgtcg cttttcatcg
    38641 ctgaaagcac ggtttttgct gcagcttccc cggcggcaat cgccagatcc ataccgcgaa
    38701 tggtaaaacc gaggttcata cacattccgg cggcatcacc ggcaatcaat acgccgtcac
    38761 caaccaactc cggcagcatg ttgatgcctg cttccggcac tacgtgagcg gaatattcca
    38821 ccagcttgcc gcccgcgatc agcggtgcca ctgccggatg ctgtttgaaa tcttccagca
    38881 tttgcggcac cgattttttt gcgtcatgca gatgatgcag accacaaacc agccccagcg
    38941 acagggtatt ttcattggta taaaggaagc cgccgcccat caggccatcg gtgggtgatc
    39001 ccgcaaacag acaagccgcg ccctgattac cctgcaactg aaaacggtct tcgattaccg
    39061 acttcggtaa ctcgatcagt tccttcacgc caaccgccac atccgtcggt ttaacgcgtt
    39121 ttgccatccc cagcttttcg gcaaggatgg agttcactcc atcagcaagg atcaccgttt
    39181 tcgcttcaat cacatcgcca tcggcttcta caccgacgac tttgccatcg cgctgtacga
    39241 ggttatcgac gcggatcccg gtaattaact gcgcgcccgc ttcttcggcc tgctccatca
    39301 gccaggcatc aaatttactg cgcaaaacgg agtaagaacg ctgggatggc gaggtttcgt
    39361 caccattgca gtagtccata gtcatcgctg acttttccgt cataaacgcg agtttttcat
    39421 gggtgatcag gcgttctacg ggggcggagt cggcgaaacc aggaataatg tgttccaggc
    39481 tgtgggcata gagacgcccg ccggtgacgt tcttggcacc tgcggaattg ccacgctcga
    39541 taactaacac ttgcgcccct tcgcgggcga gcaccagtgc ggcaaccgaa ccggcaagcc
    39601 cagcaccgac gatgatggcg tcaaagatat cttcggacat aagagctccc tgtcagcggc
    39661 agggccaaaa tgcagccctg ccagagtgga tcaacgcgct aaagctgcgg tcagcgccgg
    39721 aaggatcttc acggcgtcac caacaatgcc gtaatccgcg tactggaaga tcggcgcatt
    39781 tttatcttta ttgatggcga aaatggtttg cgatgcatta gcaccaacca tgtgctggat
    39841 ctgcccggag atccccaccg ccaggtacag ttcaggtttc agcatcaggt tggagatacc
    39901 gacatagcgt tcgtgctcca tccatttttc gttttccgcc accggacgag aacaggccaa
    39961 ctccgcacct atcgccttgc aaagctgttc tgccagcgca atgttctctt tgctgccaat
    40021 accgcgaccg acgctgacca ccagacgggc tttgtcgaga tcgacgctgt tgctctggcg
    40081 cgcctgggtt gccgtgcggg taatcgccac agccggagcc tgccactcca cggtgtgcgt
    40141 ttcgccagtg cgtgacgcgt ctggctgagc cgcatcgaac gtgccgctgc tgatggtcag
    40201 taccgcatac ggcgtggcaa tgcgttcttc gccaatcgcc agaccaccgt aaaccatgtg
    40261 tttcactgtc gctttaccgt cctgtacgct gacggtgctg gcatcgttag acaccgccgc
    40321 tttaaggcga taacccagtt ttgccgccag taatttgccg cgacgggtgt ttggcagcag
    40381 caccaggccg tctgcgccgt gctggcgaat agtgtcagcc atgacaccgg cgtaatcttc
    40441 gatcatccga tcgtccggtt tgccgtttaa tttccagaca tgattagcgc cgagctggat
    40501 tgcctgtacg ccgtcggcat cattgaggac aaaggtgttg atttgattag ctaaagcctg
    40561 cgcaccgttc atcagttccg gcagacgaga aggggtatcg ctgaataccc agacttgaga
    40621 aaacgtgttc atagcatccc ctgtaattaa atgactttgc gaagattttc agcaaatgcg
    40681 gcgatctgtt cttcgccgtc gccttcaatc acgatgcgct gacgttcgcg ctgtttcggc
    40741 gcagcaacct gttgttcaga ccaggcgtcc acggcgttaa aaccgatatc cgccgccgac
    40801 catacctgga ctggcttttt cgccgcgccg agaatggctt tcatcgaagg aatttgtggg
    40861 gagttgatat cagtggaaac agcaacaacc gcaggcagcg gaatgcttaa ggtttcggtt
    40921 tcatcttcca gttcgcgctc aacggtgagg gtatctgcag tcagggagat aattttgctg
    40981 acgccgttaa ctgccggaat attgaggatt tcgcccacca gcagaccaac ctgctgggca
    41041 taaaggtcgg aagaaccatc gccacagagg atcaaatcaa agcctgcttt ctgggcggct
    41101 gcagccagtg cgctcgccgt ttgttgcggc agtgcctgct cgaactggtc atcaatcacc
    41161 acaatcagtt catccgggcc gcgcgatagc acatctttac gccctttggc gttggtcagg
    41221 gctttaccgc ccacacttaa ggccgtcacc tgcgcctctg ctgcctgttg ctttagctgg
    41281 caagccgctt caatagcgtt cagatcgtat tggcttattt tggcatcggc tttgctgaag
    41341 tctaatgaac catcagcatt attgaccgca atatcctgtt catcaggcac gcacttatag
    41401 caagtaataa tcttcattgc atctccagaa atcatgaagg aaacgtcgtt ca
//
LOCUS       DAZKHF020000013        41259 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2201S31241
            SAMN43267800-rid25106413.denovo.013, whole genome shotgun sequence.
ACCESSION   DAZKHF020000013 DAZKHF020000000
VERSION     DAZKHF020000013.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267800
            Sequence Read Archive: SRR30307263
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 41259)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 41259)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 41259)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350581.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350581.2
            Genome Coverage       :: 151x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:21:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,652
            CDSs (total)                      :: 5,557
            Genes (coding)                    :: 5,320
            CDSs (with protein)               :: 5,320
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 237
            CDSs (without protein)            :: 237
            Pseudo Genes (ambiguous residues) :: 0 of 237
            Pseudo Genes (frameshifted)       :: 102 of 237
            Pseudo Genes (incomplete)         :: 130 of 237
            Pseudo Genes (internal stop)      :: 52 of 237
            Pseudo Genes (multiple problems)  :: 41 of 237
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..41259
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267800-rid25106413.denovo.013"
                     /serotype="O 26:H11"
                     /isolate="2201S31241"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-01"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            complement(219..431)
                     /gene="rpmE"
                     /locus_tag="ACHOSC_RS02800"
                     /old_locus_tag="pgaptmp_000560"
     CDS             complement(219..431)
                     /gene="rpmE"
                     /locus_tag="ACHOSC_RS02800"
                     /old_locus_tag="pgaptmp_000560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L31"
                     /protein_id="HGU4546561.1"
                     /translation="MKKDIHPKYEEITASCSCGNVMKIRSTVGHDLNLDVCSKCHPFF
                     TGKQRDVATGGRVDRFNKRFNIPGSK"
     gene            634..2832
                     /gene="priA"
                     /locus_tag="ACHOSC_RS02805"
                     /old_locus_tag="pgaptmp_000561"
     CDS             634..2832
                     /gene="priA"
                     /locus_tag="ACHOSC_RS02805"
                     /old_locus_tag="pgaptmp_000561"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709739.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein N'"
                     /protein_id="HGU4546562.1"
                     /translation="MPVAHVALPVPLPRTFDYLLPEGMTVKAGCRVRVPFGKQQERIG
                     IVVSVSDASELPLNELKAVVEVLDVEPVFTHSVWRLLLWAADYYHHPIGDVLFHALPI
                     LLRQGRPAANAPMWYWFATEQGQAVDLNSLKRSPKQQQALAALRQGKIWRDQVAELEF
                     NDAALQALRKKGLCDLASETPEFSDWRTNYAVSGERLRLNTEQATAVGAIHSAADTFS
                     AWLLAGVTGSGKTEVYLSVLENVLAQGKQALVMVPEIGLTPQTIARFRERFNAPVEVL
                     HSGLNDSERLSAWLKAKNGEAAIVIGTRSALFTPFKNLGVIVIDEEHDSSYKQQEGWR
                     YHARDLAVYRAHSEQIPIILGSATPALETLCNVQQKKYRLLRLTRRAGNARPAIQHVL
                     DLKGQKVQAGLAPALITRMRQHLQANNQVILFLNRRGFAPALLCHDCGWIAECPRCDH
                     YYTLHQAQQHLRCHHCDSQRPVPRQCPSCGSTHLVPVGLGTEQLEQTLAPMFPGVPIS
                     RIDRDTTSRKGALEQQLAEVHRGGARILIGTQMLAKGHHFPDVTLVALLDVDGALFSA
                     DFRSAERFAQLYTQVAGRAGRAGKQGEVVLQTHHPEHPLLQTLLYKGYDAFAEQALAE
                     RRMMQLPPWTSHVIVRAEDHNNQHAPLFLQQLRNLILSSPLADDKLWVLGPVPALAPK
                     RGGRWRWQILLQHPSRVRLQHIISGTLALINTIPDSRKVKWVLDVDPIEG"
     gene            2988..4013
                     /gene="cytR"
                     /locus_tag="ACHOSC_RS02810"
                     /old_locus_tag="pgaptmp_000562"
     CDS             2988..4013
                     /gene="cytR"
                     /locus_tag="ACHOSC_RS02810"
                     /old_locus_tag="pgaptmp_000562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator CytR"
                     /protein_id="HGU4546563.1"
                     /translation="MKAKKQETAATMKDVALKAKVSTATVSRALMNPDKVSQATRNRV
                     EKAAREVGYLPQPMGRNVKRNESRTILVIVPDICDPFFSEIIRGIEVTAANHGYLVLI
                     GDCAHQNQQEKTFIDLIITKQIDGMLLLGSRLPFDASIEEQRNLPPMVMANEFAPELE
                     LPTVHIDNLTAAFDAVNYLYEQGHKRIGCIAGPEEMPLCHYRLQGYVQALRRCGIMVD
                     PQYIARGDFTFEAGSKAMQQLLDLPQPPTAVFCHSDVMALGALSQAKRQGLKVPEDLS
                     IIGFDNIDLTQFCDPPLTTIAQPRYEIGREAMLLLLDQMQGQHVGSGSRLMDCELIIR
                     GSTRALP"
     gene            4105..5064
                     /gene="ftsN"
                     /locus_tag="ACHOSC_RS02815"
                     /old_locus_tag="pgaptmp_000563"
     CDS             4105..5064
                     /gene="ftsN"
                     /locus_tag="ACHOSC_RS02815"
                     /old_locus_tag="pgaptmp_000563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsN"
                     /protein_id="HGU4546564.1"
                     /translation="MAQRDYVRRSQPAPSRRKKSTSRKKQRNLPAVSPAMVAIAAAVL
                     VTFIGGLYFITHHKKEESETLQSQKVTGNGLPPKPEERWRYIKELESRQPGVRAPTEP
                     SAGGEVKTPEQLTPEQRQLLEQMQADMRQQPTQLVEVPWNEQTPEQRQQTLQRQRQAQ
                     QLAEQQRLAQQSRTTEQSWQQQTRTSQAAPVQAQPRQSKPASTQQPYQDLLQTPAHTT
                     AQSKPQQAAPVARAADAPKPTAEKKDERRWMVQCGSFRGAEQAETVRAQLAFEGFDSK
                     ITTNNGWNRVVIGPVKGKENADSTLNRLKMAGHTNCIRLAAGG"
     gene            5157..5687
                     /gene="hslV"
                     /locus_tag="ACHOSC_RS02820"
                     /old_locus_tag="pgaptmp_000564"
     CDS             5157..5687
                     /gene="hslV"
                     /locus_tag="ACHOSC_RS02820"
                     /old_locus_tag="pgaptmp_000564"
                     /EC_number="3.4.25.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009376 - HslUV protease complex
                     [Evidence IEA];
                     GO_function: GO:0004298 - threonine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0051603 - proteolysis involved in protein
                     catabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent protease subunit HslV"
                     /protein_id="HGU4546565.1"
                     /translation="MTTIVSVRRNGHVVIAGDGQATLGNTVMKGNVKKVRRLYNDKVI
                     AGFAGGTADAFTLFELFERKLEMHQGHLVKAAVELAKDWRTDRMLRKLEALLAVADET
                     ASLIITGNGDVVQPENDLIAIGSGGPYAQAAARALLENTELSAREIAEKALDIAGDIC
                     IYTNHFHTIEELSYKA"
     gene            5697..7028
                     /gene="hslU"
                     /locus_tag="ACHOSC_RS02825"
                     /old_locus_tag="pgaptmp_000565"
     CDS             5697..7028
                     /gene="hslU"
                     /locus_tag="ACHOSC_RS02825"
                     /old_locus_tag="pgaptmp_000565"
                     /EC_number="3.4.25.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019841241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009376 - HslUV protease complex
                     [Evidence IEA];
                     GO_function: GO:0004176 - ATP-dependent peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HslU--HslV peptidase ATPase subunit"
                     /protein_id="HGU4546566.1"
                     /translation="MSEMTPREIVSELDKHIIGQDNAKRSVAIALRNRWRRMQLNEEL
                     RHEVTPKNILMIGPTGVGKTEIARRLAKLANAPFIKVEATKFTEVGYVGKEVDSIIRD
                     LTDAAVKMVRVQAIEKNRYRAEELAEERILDVLIPPAKNNWGQTEQQQEPSAARQAFR
                     KKLREGQLDDKEIEIDLAAAPMGVEIMAPPGMEEMTSQLQSMFQNLGGQKQKARKLKI
                     KDAMKLLIEEEAAKLVNPEELKQDAIDAVEQHGIVFIDEIDKICKRGESSGPDVSREG
                     VQRDLLPLVEGCTVSTKHGMVKTDHILFIASGAFQIAKPSDLIPELQGRLPIRVELQA
                     LTTSDFERILTEPNASITVQYKALMATEGVNIEFTDSGIKRIAEAAWQVNESTENIGA
                     RRLHTVLERLMEEISYDASDLSGQNITIDADYVSKHLDALVADEDLSRFIL"
     gene            7095..8021
                     /gene="menA"
                     /locus_tag="ACHOSC_RS02830"
                     /old_locus_tag="pgaptmp_000566"
     CDS             7095..8021
                     /gene="menA"
                     /locus_tag="ACHOSC_RS02830"
                     /old_locus_tag="pgaptmp_000566"
                     /EC_number="2.5.1.74"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-dihydroxy-2-naphthoate
                     polyprenyltransferase"
                     /protein_id="HGU4546567.1"
                     /translation="MTEQQISRTQAWLESLRPKTLPLAFAAIIVGTALAWWQGHFDPL
                     VALLALITAGLLQILSNLANDYGDAVKGSDKPDRIGPLRGMQKGVITQQEMKRALIIT
                     VVLICLSGLALVAVACHTLADFVGFLILGGLSIIAAITYTVGNRPYGYIGLGDISVLV
                     FFGWLSVMGSWYLQAHTLIPALILPATACGLLATAVLNINNLRDINSDRENGKNTLVV
                     RLGEVNARRYHACLLMGSLVCLALFNLFSLHSLWGWLFLLAAPLLVKQARYVMREMDP
                     VAMRPMLERTVKGALLTNLLFVLGIFLSQWAA"
     gene            8114..8599
                     /gene="rraA"
                     /locus_tag="ACHOSC_RS02835"
                     /old_locus_tag="pgaptmp_000567"
     CDS             8114..8599
                     /gene="rraA"
                     /locus_tag="ACHOSC_RS02835"
                     /old_locus_tag="pgaptmp_000567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001392924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008428 - ribonuclease inhibitor activity
                     [Evidence IEA];
                     GO_process: GO:0051252 - regulation of RNA metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease E activity regulator RraA"
                     /protein_id="HGU4546568.1"
                     /translation="MKYDTSELCDIYQEDVNVVEPLFSNFGGRASFGGQIITVKCFED
                     NGLLYDLLEQNGRGRVLVVDGGGSVRRALVDAELARLAVQNEWEGLVIYGAVRQVDDL
                     EELDIGIQAMAAIPVGAAGEGIGESDVRVNFGGVTFFSGDHLYADNTGIILSEDPLDI
                     E"
     gene            complement(8684..8929)
                     /gene="zapB"
                     /locus_tag="ACHOSC_RS02840"
                     /old_locus_tag="pgaptmp_000568"
     CDS             complement(8684..8929)
                     /gene="zapB"
                     /locus_tag="ACHOSC_RS02840"
                     /old_locus_tag="pgaptmp_000568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septal ring assembly protein ZapB"
                     /protein_id="HGU4546569.1"
                     /translation="MTMSLEVFEKLEAKVQQAIDTITLLQMEIEELKEKNNSLSQEVQ
                     NAQHQREELERENNHLKEQQNGWQERLQALLGRMEEV"
     gene            9354..10199
                     /gene="glpF"
                     /locus_tag="ACHOSC_RS02845"
                     /old_locus_tag="pgaptmp_000569"
     CDS             9354..10199
                     /gene="glpF"
                     /locus_tag="ACHOSC_RS02845"
                     /old_locus_tag="pgaptmp_000569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol uptake facilitator protein GlpF"
                     /protein_id="HGU4546570.1"
                     /translation="MSQTSTLKGQCIAEFLGTGLLIFFGVGCVAALKVAGASFGQWEI
                     SVIWGLGVAMAIYLTAGVSGAHLNPAVTIALWLFACFDKRKVIPFIVSQVAGAFCAAA
                     LVYGLYYNLFFDFEQTHHIVRGSVESVDLAGTFSTYPNPHINFVQAFAVEMVITAILM
                     GLILALTDDGNGVPRGPLAPLLIGLLIAVIGASMGPLTGFAMNPARDFGPKVFAWLAG
                     WGNVAFTGGRDIPYFLVPLFGPIVGAIVGAFAYRKLIGRHLPCDICVVEEKETTTPSE
                     QKASL"
     gene            10222..11730
                     /gene="glpK"
                     /locus_tag="ACHOSC_RS02850"
                     /old_locus_tag="pgaptmp_000570"
     CDS             10222..11730
                     /gene="glpK"
                     /locus_tag="ACHOSC_RS02850"
                     /old_locus_tag="pgaptmp_000570"
                     /EC_number="2.7.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004370 - glycerol kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006072 - glycerol-3-phosphate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol kinase GlpK"
                     /protein_id="HGU4546571.1"
                     /translation="MTEKKYIVALDQGTTSSRAVVMDHDANIISVSQREFEQIYPKPG
                     WVEHDPMEIWATQSSTLVEVLAKADISSDQIAAIGITNQRETTIVWEKETGKPIYNAI
                     VWQCRRTAEICEHLKRDGLEDYIRSNTGLVIDPYFSGTKVKWILDHVEGSRERARRGE
                     LLFGTVDTWLIWKMTQGRVHVTDYTNASRTMLFNIHTLDWDDKMLEVLDIPREMLPEV
                     RRSSEVYGQTNIGGKGGTRIPISGIAGDQQAALFGQLCVKEGMAKNTYGTGCFMLMNT
                     GEKAVKSENGLLTTIACGPTGEVNYALEGAVFMAGASIQWLRDEMKLINDAYDSEYFA
                     TKVQNTNGVYVVPAFTGLGAPYWDPYARGAIFGLTRGVNANHIIRATLESIAYQTRDV
                     LEAMQADSGIRLHALRVDGGAVANNFLMQFQSDILGTRVERPEVREVTALGAAYLAGL
                     AVGFWQNLDELQEKAVIEREFRPGIETTERNYRYAGWKKAVKRAMAWEEHDE"
     gene            11865..12875
                     /gene="glpX"
                     /locus_tag="ACHOSC_RS02855"
                     /old_locus_tag="pgaptmp_000571"
     CDS             11865..12875
                     /gene="glpX"
                     /locus_tag="ACHOSC_RS02855"
                     /old_locus_tag="pgaptmp_000571"
                     /EC_number="3.1.3.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042132 - fructose 1,6-bisphosphate
                     1-phosphatase activity [Evidence IEA];
                     GO_process: GO:0006071 - glycerol metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class II fructose-bisphosphatase"
                     /protein_id="HGU4546572.1"
                     /translation="MRRELAIEFSRVTESAALAGYKWLGRGDKNTADGAAVNAMRIML
                     NQVNIDGTIVIGEGEIDEAPMLYIGEKVGTGRGDAVDIAVDPIEGTRMTAMGQANALA
                     VLAVGDKGCFLNAPDMYMEKLIVGPGAKGTIDLNLPLADNLRNVAAALGKPLSELTVT
                     ILAKPRHDAVIAEMQQLGVRVFAIPDGDVAASILTCMPDSEVDVLYGIGGAPEGVVSA
                     AVIRALDGDMNGRLLARHDVKGDNEENRRIGEQELARCKAMGIEAGKVLRLGDMARSD
                     NVIFSATGITKGDLLEGISRKGNIATTETLLIRGKSRTIRRIQSIHYLDRKDPEMQVH
                     IL"
     gene            12972..13718
                     /gene="fpr"
                     /locus_tag="ACHOSC_RS02860"
                     /old_locus_tag="pgaptmp_000572"
     CDS             12972..13718
                     /gene="fpr"
                     /locus_tag="ACHOSC_RS02860"
                     /old_locus_tag="pgaptmp_000572"
                     /EC_number="1.18.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004324 - ferredoxin-NADP+ reductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin--NADP(+) reductase"
                     /protein_id="HGU4546573.1"
                     /translation="MADWVTGKVTKVQNWTDALFSLTVHAPVLPFTAGQFTKLGLEID
                     GERVQRAYSYVNSPDNPDLEFYLVTVPDGKLSPRLAALKPGDEVQVVSEAAGFFVLDE
                     VPDCETLWMLATGTAIGPYLSILQLGKDLDRFKNLVLVHAARYAADLSYLPLMQELEK
                     RYEGKLRIQTVVSRETAAGSLTGRIPALIESGELESAIGLPMNKETSHVMLCGNPQMV
                     RDTQQLLKETRQMTKHLRRRPGHMTAEHYW"
     gene            complement(13723..14151)
                     /gene="uspD"
                     /locus_tag="ACHOSC_RS02865"
                     /old_locus_tag="pgaptmp_000573"
     CDS             complement(13723..14151)
                     /gene="uspD"
                     /locus_tag="ACHOSC_RS02865"
                     /old_locus_tag="pgaptmp_000573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein UspD"
                     /protein_id="HGU4546574.1"
                     /translation="MAYKHIGVAISGNEEDALLVNKALELARHNDAHLTLIHIDDGLS
                     ELYPGIYFPATEDILQLLKNKSDNKLYKLTKNIQWPKTKLRIERGEMPETLLEIMQKE
                     QCDLLVCGHHHSFINRLMPAYRGMINKMSADLLIVPFIDK"
     gene            complement(14178..14477)
                     /gene="yiiS"
                     /locus_tag="ACHOSC_RS02870"
                     /old_locus_tag="pgaptmp_000574"
     CDS             complement(14178..14477)
                     /gene="yiiS"
                     /locus_tag="ACHOSC_RS02870"
                     /old_locus_tag="pgaptmp_000574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF406 domain-containing protein"
                     /protein_id="HGU4546575.1"
                     /translation="MKDVVDKCSTKGCAIDIGTVIDNDNCTSKFSRFFATREEAESFM
                     TKLKELAAAASSADEGASVAYKIKDLEGQVELDAAFTFSCQAEMIIFELSLRSLA"
     gene            complement(14689..15129)
                     /gene="yiiR"
                     /locus_tag="ACHOSC_RS02875"
                     /old_locus_tag="pgaptmp_000575"
     CDS             complement(14689..15129)
                     /gene="yiiR"
                     /locus_tag="ACHOSC_RS02875"
                     /old_locus_tag="pgaptmp_000575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF805 domain-containing protein"
                     /protein_id="HGU4546576.1"
                     /translation="MTIQQWLFSFKGRIGRRDFWIWIGLWFAGMLVLFSLAGKNLLDI
                     QTAAFCLVCLLWPTAAVTVKRLHDRGRSGAWAFLMIVAWMLLAGNWAILPGVWQWAVG
                     RFVPTLILVMMLIDLGAFVGTQGENKYGKDTQDVKYKADNKSSN"
     gene            15230..15829
                     /gene="yiiQ"
                     /locus_tag="ACHOSC_RS02880"
                     /old_locus_tag="pgaptmp_000576"
     CDS             15230..15829
                     /gene="yiiQ"
                     /locus_tag="ACHOSC_RS02880"
                     /old_locus_tag="pgaptmp_000576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YiiQ family protein"
                     /protein_id="HGU4546577.1"
                     /translation="MKPGCTLFFLLCSALTVTTTAHAQTPDTATTAPYLLAGAPTFDL
                     SISQFREDFNSQNPSLPLNEFRAIDSSPDKANLTRAASKINENLYASTALERGTLKIK
                     SIQMTWLPIQGPEQKAAKAKAQEYMAAVIRTLTPLMTKTQSQKKLQSLLTAGKNKRYY
                     TETEGALRYVVADNGEKGLTFAVEPIKLALSESLEGLNK"
     gene            15937..16704
                     /gene="tpiA"
                     /locus_tag="ACHOSC_RS02885"
                     /old_locus_tag="pgaptmp_000577"
     CDS             15937..16704
                     /gene="tpiA"
                     /locus_tag="ACHOSC_RS02885"
                     /old_locus_tag="pgaptmp_000577"
                     /EC_number="5.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004807 - triose-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="triose-phosphate isomerase"
                     /protein_id="HGU4546578.1"
                     /translation="MRHPLVMGNWKLNGSRHMVHELVSNLRKELAGVAGCAVAIAPPE
                     MYIDMAKREAEGSHIMLGAQNVDLNLSGAFTGETSAAMLKDIGAQYIIIGHSERRTYH
                     KESDELIAKKFAVLKEQGLTPVLCIGETEAENEAGKTEEVCARQIDAVLKTQGAAAFE
                     GAVIAYEPVWAIGTGKSATPAQAQAVHKFIRDHIAKVDANIAEQVIIQYGGSVNASNA
                     AELFAQPDIDGALVGGASLKADAFAVIVKAAEAAKQA"
     gene            complement(16759..17514)
                     /gene="cdh"
                     /locus_tag="ACHOSC_RS02890"
                     /old_locus_tag="pgaptmp_000578"
     CDS             complement(16759..17514)
                     /gene="cdh"
                     /locus_tag="ACHOSC_RS02890"
                     /old_locus_tag="pgaptmp_000578"
                     /EC_number="3.6.1.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709723.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008715 - CDP-diacylglycerol diphosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol diphosphatase"
                     /protein_id="HGU4546579.1"
                     /translation="MKKAGLLFLVMIVIAVVAAGIGYWKLTGEESDTLRKIVLEECLP
                     NQQQNQNPSPCAEVKPNAGYVVLKDLNGPLQYLLMPTYRINGTESPLLTDPSTPNFFW
                     LAWQARDFMSKKYGQPVPDRAVSLAINSRTGRTQNHFHIHISCIRPDVREQLDKNLAN
                     ISSRWLPLPGGLRGHEYLARRVTESELVQRSPFMMLAEEVPEAREHMGSYGLAMVRQS
                     DNSFVLLATQRNLLTLNRASAEEIQDHECEILR"
     gene            complement(17621..18610)
                     /gene="sbp"
                     /locus_tag="ACHOSC_RS02895"
                     /old_locus_tag="pgaptmp_000579"
     CDS             complement(17621..18610)
                     /gene="sbp"
                     /locus_tag="ACHOSC_RS02895"
                     /old_locus_tag="pgaptmp_000579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005133064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate/thiosulfate ABC transporter
                     substrate-binding protein Sbp"
                     /protein_id="HGU4546580.1"
                     /translation="MNKWGVGLTFLLAATNVMAKDIQLLNVSYDPTRELYEQYNKAFS
                     AHWKQQTGDNVVIRQSHGGSGKQATSVINGIEADVVTLALAYDVDAIAERGRIDKEWI
                     KRLPDNSAPYTSTIVFLVRKGNPKQIHDWNDLIKPGVSVITPNPKSSGGARWNYLAAW
                     GYALHHNNNDQAKAQDFVRALYKNVEVLDSGARGSTNTFVERGIGDVLIARENEALLV
                     ANELGKDKFEIVTPSESILAEPTVSVVDKVVEKKGTKEVAEAYLKYLYSPEGQEIAAK
                     NYYRPRDAEVAKKYENAFPKLKLFTIDEEFGGWTKAQKEHFANGGTFDQISKR"
     gene            complement(18929..19891)
                     /gene="pfkA"
                     /locus_tag="ACHOSC_RS02900"
                     /old_locus_tag="pgaptmp_000580"
     CDS             complement(18929..19891)
                     /gene="pfkA"
                     /locus_tag="ACHOSC_RS02900"
                     /old_locus_tag="pgaptmp_000580"
                     /EC_number="2.7.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014072359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006002 - fructose 6-phosphate metabolic
                     process [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphofructokinase"
                     /protein_id="HGU4546581.1"
                     /translation="MIKKIGVLTSGGDAPGMNAAIRGVVRSALTEGLEVMGIYDGYLG
                     LYEDRMVQLDRYSVSDMINRGGTFLGSARFPEFRDENIRAVAIENLKKRGIDALVVIG
                     GDGSYMGAMRLTEMGFPCIGLPGTIDNDIKGTDYTIGFFTALSTVVEAIDRLRDTSSS
                     HQRISVVEVMGRYCGDLTLAAAIAGGCEFVVVPEVEFSREDLVNEIKAGIAKGKKHAI
                     VAITEHMCDVDELAHFIEKETGRETRATVLGHIQRGGSPVPYDRILASRMGAYAIDLL
                     LAGYGGRCVGIQNEQLVHHDIIDAIENMKRPFKGDWLDCAKKLY"
     gene            complement(20072..20974)
                     /gene="fieF"
                     /locus_tag="ACHOSC_RS02905"
                     /old_locus_tag="pgaptmp_000581"
     CDS             complement(20072..20974)
                     /gene="fieF"
                     /locus_tag="ACHOSC_RS02905"
                     /old_locus_tag="pgaptmp_000581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312867.1"
                     /note="FieF, a metal efflux transporter, is a member of
                     the CDF (cation diffusion facilitator) family of
                     transporters; Derived by automated computational analysis
                     using gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDF family cation-efflux transporter FieF"
                     /protein_id="HGU4546582.1"
                     /translation="MNQSYGRLVSRAAIAATAMASLLLLIKIFAWWYTGSVSILAALV
                     DSLVDIGASLTNLLVVRYSLQPADDNHSFGHGKAESLAALAQSMFISGSALFLFLTGI
                     QHLISPTPMTDPGVGVIVTIVALICTIILVSFQRWVVRRTQSQAVRADMLHYQSDVMM
                     NGAILLALGLSWYGWHRADALFALGIGIYILYSALRMGYEAVQSLLDRALPDEERQEI
                     IDIVTSWPGVSGAHDLRTRQSGPTRFIQIHLEMEDSLPLVQAHMVADQVEQAILRRFP
                     GSDVIIHQDPCSVVPREGKRSMLS"
     gene            complement(21123..21623)
                     /gene="cpxP"
                     /locus_tag="ACHOSC_RS02910"
                     /old_locus_tag="pgaptmp_000582"
     CDS             complement(21123..21623)
                     /gene="cpxP"
                     /locus_tag="ACHOSC_RS02910"
                     /old_locus_tag="pgaptmp_000582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell-envelope stress modulator CpxP"
                     /protein_id="HGU4546583.1"
                     /translation="MRIVTAAVMASTLAVSSLSHAAEVGSGDNWHPGEELTQRSTQSH
                     MFDGISLTEHQRQQMRDLMQQARHEQPPVNVSELETMHRLVTAENFDENAVRAQAEKM
                     ANEQIARQVEMAKVRNQMYRLLTPEQQAVLNEKHQQRMEQLRDVTQWQKSSSLKLLSS
                     SNSRSQ"
     gene            21773..22471
                     /gene="cpxR"
                     /locus_tag="ACHOSC_RS02915"
                     /old_locus_tag="pgaptmp_000583"
     CDS             21773..22471
                     /gene="cpxR"
                     /locus_tag="ACHOSC_RS02915"
                     /old_locus_tag="pgaptmp_000583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001033718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="envelope stress response regulator transcription
                     factor CpxR"
                     /protein_id="HGU4546584.1"
                     /translation="MNKILLVDDDRELTSLLKELLEMEGFNVIVAHDGEQALDLLDDS
                     IDLLLLDVMMPKKNGIDTLKALRQTHQTPVIMLTARGSELDRVLGLELGADDYLPKPF
                     NDRELVARIRAILRRSHWSEQQQNNDNGSPTLEVDALVLNPGRQEASFDGQTLELTGT
                     EFTLLYLLAQHLGQVVSREHLSQEVLGKRLTPFDRAIDMHISNLRRKLPDRKDGHPWF
                     KTLRGRGYLMVSAS"
     gene            22468..23841
                     /gene="cpxA"
                     /locus_tag="ACHOSC_RS02920"
                     /old_locus_tag="pgaptmp_000584"
     CDS             22468..23841
                     /gene="cpxA"
                     /locus_tag="ACHOSC_RS02920"
                     /old_locus_tag="pgaptmp_000584"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="envelope stress sensor histidine kinase CpxA"
                     /protein_id="HGU4546585.1"
                     /translation="MIGSLTARIFAIFWLTLALVLMLVLMLPKLDSRQMTELLDSEQR
                     QGLMIEQHVEAELANDPPNDLMWWRRLFRAIDKWAPPGQRLLLVTTEGRVIGAERSEM
                     QIIRNFIGQADNADHPQKKKYGRVELVGPFSVRDGEDNYQLYLIRPASSSQSDFINLL
                     FDRPLLLLIVTMLVSTPLLLWLAWSLAKPARKLKNAADEVAQGNLRQHPELEAGPQEF
                     LAAGASFNQMVTALERMMTSQQRLLSDISHELRTPLTRLQLGTALLRRRSGESKELER
                     IETEAQRLDSMINDLLVMSRNQQKNALVSETIKANQLWSEVLDNAAFEAEQMGKSLTV
                     NFPPGPWPLYGNPNALESALENIVRNALRYSHTKIEVGFAVDKDGITITVDDDGPGVS
                     PEDREQIFRPFYRTDEARDRESGGTGLGLAIVETAIQQHRGWVKAEDSPLGGLRLVIW
                     LPLYKRS"
     gene            complement(23983..24657)
                     /gene="yiiM"
                     /locus_tag="ACHOSC_RS02925"
                     /old_locus_tag="pgaptmp_000585"
     CDS             complement(23983..24657)
                     /gene="yiiM"
                     /locus_tag="ACHOSC_RS02925"
                     /old_locus_tag="pgaptmp_000585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709716.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030151 - molybdenum ion binding [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-hydroxyaminopurine reductase"
                     /protein_id="HGU4546586.1"
                     /translation="MRYPVDVYTGKIQAYPEGKPSAIAKIQVDGELMLTELGLEGDEQ
                     AEKKVHGGPDRALCHYPREHYLYWVREFPEQAELFVAPAFGENLSTDGLTESNVHMGD
                     IFRWGEALIQVSQPRSPCYKLNYHFDISDIAQLMQNTGKVGWLYSVIAPGKVSADAPL
                     ELVSRVSDVTVQEAAAIAWHMPFDDDQYHRLLSAAGLSKSWTRTMQKRRLSGKIEDFS
                     RRLWGK"
     gene            complement(24806..25789)
                     /gene="kdgT"
                     /locus_tag="ACHOSC_RS02930"
                     /old_locus_tag="pgaptmp_000586"
     CDS             complement(24806..25789)
                     /gene="kdgT"
                     /locus_tag="ACHOSC_RS02930"
                     /old_locus_tag="pgaptmp_000586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709713.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015649 - 2-keto-3-deoxygluconate:proton
                     symporter activity [Evidence IEA];
                     GO_process: GO:0046411 - 2-keto-3-deoxygluconate
                     transmembrane transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-keto-3-deoxygluconate transporter"
                     /protein_id="HGU4546587.1"
                     /translation="MQIKRSIEKIPGGMMLVPLFLGALCHTFSPGAGKYFGSFTNGMI
                     TGTVPILAVWFFCMGASIKLSATGTVLRKSGTLVVTKIAVAWVVAAIASRIIPEHGVE
                     VGFFAGLSTLALVAAMDMTNGGLYASIMQQYGTKEEAGAFVLMSLESGPLMTMIILGT
                     AGIASFEPHVFVGAVLPFLVGFALGNLDPELREFFSKAVQTLIPFFAFALGNTIDLTV
                     IAQTGLLGILLGVAVIIVTGIPLIIADKLIGGGDGTAGIAASSSAGAAVATPVLIAEM
                     VPAFKPMAPAATSLVATAVIVTSILVPILTSIWSRKVKARAAKIEILGTVK"
     gene            complement(26049..26669)
                     /gene="sodA"
                     /locus_tag="ACHOSC_RS02935"
                     /old_locus_tag="pgaptmp_000587"
     CDS             complement(26049..26669)
                     /gene="sodA"
                     /locus_tag="ACHOSC_RS02935"
                     /old_locus_tag="pgaptmp_000587"
                     /EC_number="1.15.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010345169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004784 - superoxide dismutase activity
                     [Evidence IEA];
                     GO_process: GO:0006801 - superoxide metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase [Mn]"
                     /protein_id="HGU4546588.1"
                     /translation="MSYTLPSLPYAYDALEPHFDKQTMEIHHTKHHQTYVNNANAALE
                     SLPEFANLPVEELITKLDQLPADKKTVLRNNAGGHANHSLFWKGLKKGTTLQGDLKAA
                     IERDFGSVDNFKAEFEKAAASRFGSGWAWLVLKGDKLAVVSTANQDSPLMGEAISGAS
                     GFPILGLDVWEHAYYLKFQNRRPDYIKEFWNVVNWDEAAARFAAKK"
     gene            26954..27988
                     /gene="rhaT"
                     /locus_tag="ACHOSC_RS02940"
                     /old_locus_tag="pgaptmp_000588"
     CDS             26954..27988
                     /gene="rhaT"
                     /locus_tag="ACHOSC_RS02940"
                     /old_locus_tag="pgaptmp_000588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015153 - rhamnose transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0008645 - hexose transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-rhamnose/proton symporter RhaT"
                     /protein_id="HGU4546589.1"
                     /translation="MSNAITMGIFWHLIGAASAACFYAPFKKVKKWSWETMWSVGGIV
                     SWIILPWAISALLLPDFWAYYSSFSLSTLLPVFLFGAMWGIGNINYGLTMRYLGMSMG
                     IGIAIGITLIVGTLMTPIINGNFDVLINTEGGRMTLLGVLVALIGVGIVTRAGQLKER
                     KMGIKAEEFNLKKGLVLAVMCGIFSAGMSFAMNAAKPMHEAAAALGVDPLYVALPSYV
                     VIMGGGAIINLGFCFIRLAKVKDLSLKADFSLAKPLIIHNVLLSALGGLMWYLQFFFY
                     AWGHARIPAQYDYISWMLHMSFYVLCGGIVGLVLKEWSNAGRRPVTVLSLGCVVIIVA
                     ANIVGIGMAN"
     gene            complement(27985..28833)
                     /gene="rhaR"
                     /locus_tag="ACHOSC_RS02945"
                     /old_locus_tag="pgaptmp_000589"
     CDS             complement(27985..28833)
                     /gene="rhaR"
                     /locus_tag="ACHOSC_RS02945"
                     /old_locus_tag="pgaptmp_000589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418342.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional activator RhaR"
                     /protein_id="HGU4546590.1"
                     /translation="MAHQLKLLKDDFFASDQQAVAVADRYPQHVFAEHTHDFCELVIV
                     WRGNGLHVLNDRPYRITRGDLFYIHADDKHSYASVNDLVLQNIIYCPERLKLNLDWQG
                     AIPGFSASAGQPHWRLGSVGMAQARQVIGQLEHESSQHVPFANEMAELLFGQLAMLLN
                     RHRYTSDSLPPTSSETLLDKLITRLAASLKSPFALDKFCDEASCSERVLRQQFRQQTG
                     MTINQYLRQVRVCHAQYLLQHSRLLISDISTECGFEDSNYFSVVFTRETGMTPSQWRH
                     LNSQKD"
     gene            complement(28907..29744)
                     /gene="rhaS"
                     /locus_tag="ACHOSC_RS02950"
                     /old_locus_tag="pgaptmp_000590"
                     /pseudo
     CDS             complement(28907..29744)
                     /gene="rhaS"
                     /locus_tag="ACHOSC_RS02950"
                     /old_locus_tag="pgaptmp_000590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312859.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional activator RhaS"
     gene            30032..31501
                     /gene="rhaB"
                     /locus_tag="ACHOSC_RS02955"
                     /old_locus_tag="pgaptmp_000591"
     CDS             30032..31501
                     /gene="rhaB"
                     /locus_tag="ACHOSC_RS02955"
                     /old_locus_tag="pgaptmp_000591"
                     /EC_number="2.7.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008993 - rhamnulokinase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0019301 - rhamnose catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rhamnulokinase"
                     /protein_id="HGU4546591.1"
                     /translation="MTFRNCVAVNLGASSGRVMLARYERECRSLTLREIHRFKNGLHS
                     QNGYVTWDVDSLESAIRLGLNKVCEEGIRIDSIGIDTWGVDFVLLDQQGQRVGLPVAY
                     RDSRTNGLMAQAQQQLGKRDIYQRSGIQFLPFNTLYQLRALTEQQPELIPHIAHALLM
                     PDYFSYRLTGKMNWEYTNATTTQLVNINSDDWDESLLAWSGANKAWFGRPTHPGNVIG
                     HWICPQGNEIPVVAVASHDTASAVIASPLNGSRAAYLSSGTWSLMGFESQTPFTNDTA
                     LAANITNEGGAEGRYRVLKNIMGLWLLQRVLQERQINDLPALIAATQALPACRFIINP
                     NDDRFINPEAMCSEIQAACRETAQPIPESDAELARCIFDSLALLYADVLHELAQLRGE
                     DFSQLHIVGGGCQNTLLNQLCADACGIRVIAGPVEASTLGNIGIQLMTLDELNNVDNF
                     RQVVSTTANLTTFTPNPDSEIAHYVAQIHSTRQTKELCA"
     gene            31498..32757
                     /gene="rhaA"
                     /locus_tag="ACHOSC_RS02960"
                     /old_locus_tag="pgaptmp_000592"
     CDS             31498..32757
                     /gene="rhaA"
                     /locus_tag="ACHOSC_RS02960"
                     /old_locus_tag="pgaptmp_000592"
                     /EC_number="5.3.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008740 - L-rhamnose isomerase activity
                     [Evidence IEA];
                     GO_function: GO:0030145 - manganese ion binding [Evidence
                     IEA];
                     GO_process: GO:0019299 - rhamnose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-rhamnose isomerase"
                     /protein_id="HGU4546592.1"
                     /translation="MTTQLEQAWELAKQRFAAVGIDVEEALRQLDRLPVSMHCWQGDD
                     VSGFENPEGSLTGGIQATGNYPGKARNASELRTDLEQAMRLIPGPKRLNLHAIYLESD
                     TPVSRDQIKPEHFKNWVEWAKANQLGLDFNPSCFSHPLSADGFTLSHADDSIRQFWID
                     HCKASRRVSAYFGEQLGTPSVMNIWIPDGMKDITVDRLAPRQRLLAALDEVISEKLNP
                     AHHIDAVESKLFGIGAESYTVGSNEFYLGYATSRQTALCLDAGHFHPTEVISDKISAA
                     MLYVPQLLLHVSRPVRWDSDHVVLLDDETQAIASEIVRHDLFDRVHIGLDFFDASINR
                     IAAWVIGTRNMKKALLRALLEPTAELRKLEAAGDYTARQALLEEQKSLPWQAVWEMYC
                     QRHDTPAGSEWLESVRAYEKEILSQRG"
     gene            32840..33664
                     /gene="rhaD"
                     /locus_tag="ACHOSC_RS02965"
                     /old_locus_tag="pgaptmp_000593"
     CDS             32840..33664
                     /gene="rhaD"
                     /locus_tag="ACHOSC_RS02965"
                     /old_locus_tag="pgaptmp_000593"
                     /EC_number="4.1.2.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhamnulose-1-phosphate aldolase"
                     /protein_id="HGU4546593.1"
                     /translation="MQNITQSWFVQGMIKATTDAWLKGWDERNGGNLTLRLDDADIAP
                     YHDNFHQQPRYIPLSQPMPLLANTPFIVTGSGKFFRNVQLDPAANLGVVKVDSDGAGY
                     HILWGLTNEAVPTSELPAHFLSHCERIKATNGKDRVIMHCHATNLIALTYVLENDTAV
                     FTRQLWEGSTECLVVFPDGVGILPWMVPGTDEIGQATAQEMQKHSLVLWPFHGVFGSG
                     PTLDEAFGLIDTAEKSAEVLVKIYSMGGMKQTITREELIALGERFGVTPLASALAL"
     gene            33736..34884
                     /gene="fucO"
                     /locus_tag="ACHOSC_RS02970"
                     /old_locus_tag="pgaptmp_000594"
     CDS             33736..34884
                     /gene="fucO"
                     /locus_tag="ACHOSC_RS02970"
                     /old_locus_tag="pgaptmp_000594"
                     /EC_number="1.1.1.77"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_462925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008912 - lactaldehyde reductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lactaldehyde reductase"
                     /protein_id="HGU4546594.1"
                     /translation="MSFMLALPKISLHGAGAIGDMVNLVAHKQWGKALIVTDGQLVKL
                     GLLDSLFIALDQYEMSYHLFDEVFPNPTEELVQKGYAAFQDAACDYIIAFGGGSPIDT
                     AKAVKILTANPAPSTAYSGVGKVTNPGVPLVAINTTAGTAAEMTSNAVIIDTARQVKE
                     VIIDPNIIPDIAVDDASVMLEIPASVTAATGMDALTHAVEAYVSVGAHPLTDANALEA
                     IRLITLWLPKAVDDGHNLEAREQMAFAQYLAGMAFNSAGLGLVHALAHQPGATHNLPH
                     GVCNAILLPVIENFNRPNAIARFARIAQAMGVDTRDMSEEAASTEAINAIRALSHRVG
                     IPAGFSQLGITKEDIEGWLDKALADPCVPCNPRTASRDEVRELYLEAL"
     gene            34881..35195
                     /gene="rhaM"
                     /locus_tag="ACHOSC_RS02975"
                     /old_locus_tag="pgaptmp_000595"
     CDS             34881..35195
                     /gene="rhaM"
                     /locus_tag="ACHOSC_RS02975"
                     /old_locus_tag="pgaptmp_000595"
                     /EC_number="5.1.3.32"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0016857 - racemase and epimerase activity,
                     acting on carbohydrates and derivatives [Evidence IEA];
                     GO_function: GO:0062192 - L-rhamnose mutarotase activity
                     [Evidence IEA];
                     GO_process: GO:0019299 - rhamnose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-rhamnose mutarotase"
                     /protein_id="HGU4546595.1"
                     /translation="MIRKAFVMQVNPDAHEEYQRRHNPIWPELEAVLKSHGAHNYAIY
                     LDKARNLLFATVEIESEERWNAVASTDVCQRWWKYMTDVMPANPDNSPVSSELQEVFY
                     LP"
     gene            35508..35954
                     /gene="frvA"
                     /locus_tag="ACHOSC_RS02980"
                     /old_locus_tag="pgaptmp_000596"
     CDS             35508..35954
                     /gene="frvA"
                     /locus_tag="ACHOSC_RS02980"
                     /old_locus_tag="pgaptmp_000596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIA"
                     /protein_id="HGU4546596.1"
                     /translation="MAALTASCIDLNIQGNGAYSILKQLATMALQNGFITDSHQFLQT
                     LLLREKMHSTGFGSGIAVPHGKSACVKQPFVLFARKAQAIDWKASDGEDVNCWICLGV
                     PQSGEEDQVKIIGTLCRKIIHQDFINQLRQGDADQVLALLNQTLSS"
     gene            35965..37416
                     /gene="frvB"
                     /locus_tag="ACHOSC_RS02985"
                     /old_locus_tag="pgaptmp_000597"
     CDS             35965..37416
                     /gene="frvB"
                     /locus_tag="ACHOSC_RS02985"
                     /old_locus_tag="pgaptmp_000597"
                     /EC_number="2.7.1.202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418335.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose-like transporter subunit IIBC"
                     /protein_id="HGU4546597.1"
                     /translation="MESSLRIVAITNCPAGIAHTYMVAEALEQKARSLGHTIKVETQG
                     SSGVENRLSSEEIAAADYVILATGRGLSGDDRARFAGKKVYEIAISQALKNIDQIFSE
                     LPTNSQLFAADSGVKLGKQEVQSGSVMSHLMAGVSAALPFVIGGGILVALANMLVQFG
                     LPYTDMSKGAPSFTWVVESIGYLGFTFMIPIMGAYIASSIADKPAFAPAFLVCYLAND
                     KALLGTQSGAGFLGAVVLGLAIGYFVFWFRKVRLGKALQPLLGSMLIPFVTLLVFGVL
                     TYYVIGPVMSDLMGGLLHFLNTIPPSMKFAAAFLVGAMLAFDMGGPINKTAWFFCFSL
                     LEKHIYDWYAIVGVVALMPPVAAGLATFIAPKLFTRQEKEAASSAIVVGATVATEPAI
                     PYALAAPLPMITANTLAGGITGVLVIAFGIKRLAPGLGIFDPLIGLMSPVGSFYLVLA
                     IGLALNISFIIVLKGLWLRRKAKAAQQELVHEH"
     gene            37406..38476
                     /gene="frvX"
                     /locus_tag="ACHOSC_RS02990"
                     /old_locus_tag="pgaptmp_000598"
     CDS             37406..38476
                     /gene="frvX"
                     /locus_tag="ACHOSC_RS02990"
                     /old_locus_tag="pgaptmp_000598"
                     /EC_number="3.4.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709702.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase"
                     /protein_id="HGU4546598.1"
                     /translation="MNIELLQQLCEASAVSGDEQEVRDILINTLEPCVNEITFDGLGS
                     FVARKGNKGPKVAVVGHMDEVGFMVTHIDESGFLRFTTIGGWWNQSMLNHRVTIRTHK
                     GVKIPGVIGSVAPHALTEKQKQQPQSFDEMFIDIGANSRDEVEKRGVAMGDFISPEAN
                     FACWGEDKVVGKALDNRIGCAMMAELLQTVNNPEITLYGVGSVEEEVGLRGAQTSAEH
                     IKPDVVIVLDTAVAGDVPGIDNIKYPLKLGNGPGLMLFDKRYFPNQKLVAALKSCATQ
                     NDLPLQFSTMKTGATDGGRYNVMGGGRPVVALCLPTRYLHANSGMISKADYDALLTLI
                     RGFLTTLTAEKVSAFSQFRQVD"
     gene            38476..40224
                     /gene="frvR"
                     /locus_tag="ACHOSC_RS02995"
                     /old_locus_tag="pgaptmp_000599"
     CDS             38476..40224
                     /gene="frvR"
                     /locus_tag="ACHOSC_RS02995"
                     /old_locus_tag="pgaptmp_000599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative frv operon regulatory protein"
                     /protein_id="HGU4546599.1"
                     /translation="MLNERQLKIVDLLEQQPRTPGELAQQTGVSGRTILRDIDYLNFT
                     LNGKARISDSGSAGYQLEIFERRSFFQLLQKHDNDDRLLALLLLNTFTPRAQLASALN
                     LPETWVAERLPRLKQRYERTCCLASRPGLGHFIDETEEKRVILLANLLRKDPFLIPLA
                     GVTRDNLQHLSTACDNQHRWPLMQGDYLSSLILAIYALRNQLTDEWPQYPGDEIKQIV
                     EHSGLFLGDNAVRTLTGLIEKQHQQAQIISADNVQRLLQRVPGIASLNIIDTQLVENI
                     TGHLLRCLAAPVWIAEHRQSSMNNLKAAWPAAFDMSLHFITLLREQLDIPLFDSDLIG
                     LYFACALERHQNERQPIILLSDQNAIATINQLAIERDVLHCRVIIARSLSELVAIREE
                     IEPLLIINNSHYLLDDAVNNYITVKNIITAAGIEQIKHFLATAFIRQQPERFFSAPGS
                     FHYSNVRGESWQHITRQICAQLVAQHHITADEAQRIIAREGEGENLIVNRLAIPHCWS
                     EQERRFRGFFITLAQPVEVNNEVINHVLIACAAADARHELKIFSYLASILCQHPAEII
                     AGLTGYEAFMELLHKG"
     gene            complement(40274..>41259)
                     /gene="yiiG"
                     /locus_tag="ACHOSC_RS03000"
                     /old_locus_tag="pgaptmp_000600"
     CDS             complement(40274..>41259)
                     /gene="yiiG"
                     /locus_tag="ACHOSC_RS03000"
                     /old_locus_tag="pgaptmp_000600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="YiiG family protein"
                     /protein_id="HGU4546600.1"
                     /translation="TGCDDKKAETETPPPANSQPAAPAPEAPVAKAEAKPETPAQPVV
                     DEQAVFDEKMDVYIKCYNKLQIPVQSSLARYADWLKDFKQGPTGEERTVYGIYGISES
                     NLAECEKGVKSAVALTPALQPIDGVAVSYIDAAVALGNTINEMDKYYTQENYKDDAFA
                     KGKTLHQTFLKNLEAFEPVAESYHAAIQEINDKRQLAELKNIEEREGKTFHYYSLAVM
                     ISAKQINNLISQEKFDVDAAMKKVSELETLVAQAKEADKGGMNFSFINSAGQYQLEAK
                     KYVRRIRDKVPYSDWDKEQLQDANSSWMVEDSFPRALREYNEMVDDYNSLR"
BASE COUNT        10026 a        10662 c        10666 g         9905 t
ORIGIN      
        1 acaatgctac tgccatactg cgtcatgtcg ccctgacgcg ctgccggttt tccgctcatc
       61 gtaaaatcct tttgaaataa ataagaacca gaagcctagc gccagtcaac aacaggaaat
      121 gataatgagt taacaccccg gaatttttca gaatttataa aaggcaaatt acaggcacaa
      181 aaaaagcgcc gtgcggcgct tttttcggaa atccggtctt atttgctgcc cgggatgttg
      241 aaacgcttgt tgaagcggtc aacacggcca ccggtagcaa catcacgctg tttgccagtg
      301 aagaacgggt ggcacttgct gcacacgtcg aggttcaggt catgaccaac ggtggagcgg
      361 attttcatta cgttaccgca agagcagcta gcagtaattt cttcgtattt cgggtgaata
      421 tcttttttca tgggaaaacc tcggtttaag gccgcgtcgc tcttccagcc ctaacgccag
      481 acaccacgcg atgttaaaag tatagcttca atacgatcat ttcgtacgaa ggcgcgaaat
      541 catacagaaa ttaaccagcg tatgcaaact gatccgcact cttctacggc aatgtgtata
      601 ctaacccacc gaatttcaag tcaggatgat gctatgcccg tagcccacgt tgccttgccc
      661 gttccgcttc ctcgtacctt tgactatctg ctgccagaag gcatgacggt taaagctggg
      721 tgtcgcgtgc gcgtgccgtt tggcaaacag caggagcgca tcgggattgt ggtatcagtt
      781 agcgatgcca gcgaactgcc gctcaatgag ctaaaagcgg tagtcgaagt gctggacgtt
      841 gagccggtat ttactcactc cgtctggcgg ttactgctct gggcggcaga ttactatcat
      901 catccgattg gcgatgtgct gtttcatgcc ttgccgattt tactgcgcca ggggcggcct
      961 gcggcgaacg cgccgatgtg gtactggttt gccactgaac aaggtcaggc ggtggatctt
     1021 aacagcctga aacgttcgcc aaagcaacaa caggcgctgg cggcgttacg gcaaggcaaa
     1081 atctggcgcg accaggtcgc ggaacttgaa tttaatgatg ccgcgttgca ggcgctgcgc
     1141 aaaaaaggtc tgtgtgattt agcaagtgaa acaccagagt ttagcgactg gcgaacgaac
     1201 tatgccgttt ctggtgagcg gttgcgattg aataccgaac aagccaccgc cgttggcgca
     1261 attcatagcg cggcagatac tttttctgcc tggctgctgg cgggcgttac cggttccggt
     1321 aaaacggagg tttatctcag tgtactggaa aacgtgctcg ctcagggcaa acaggcgctg
     1381 gtgatggtgc cggaaatcgg cctgacaccg caaactatcg cccgttttcg tgaacgtttt
     1441 aatgcccccg tggaagttct gcattccggc ctgaacgaca gcgagcggct ttcggcgtgg
     1501 ttgaaagcga aaaatggcga ggcggcgatt gtgatcggca cccgctccgc gctgtttacg
     1561 ccgtttaaaa atctcggcgt gattgtcatt gatgaagagc acgacagctc ctacaagcag
     1621 caggaaggct ggcgctatca tgcccgcgac ctggcggtgt atcgtgcgca tagcgagcaa
     1681 atcccgatta ttcttggctc cgcaacgccc gcgctggaaa cgttatgcaa cgtccagcag
     1741 aaaaaatacc gcctgctgcg cctgacccgt cgggcaggga atgcgcgtcc ggcaattcaa
     1801 catgtgctgg atttaaaagg tcagaaggtg caggcaggtc tggctccggc gttaatcacc
     1861 cgtatgcgcc agcatttaca ggccaacaat caggttatcc tctttcttaa tcgtcggggt
     1921 tttgcgcctg cgctgctgtg ccacgactgc ggctggatag ccgaatgccc acgttgcgat
     1981 cactactaca cgctgcatca ggcgcaacag catttgcgct gtcaccactg tgacagtcag
     2041 cgtccggtgc cgcgccagtg cccttcctgc ggttccacgc acctggtccc cgtggggctg
     2101 ggcaccgaac agcttgaaca gacgcttgcg ccgatgttcc ccggcgtgcc catttcacgt
     2161 atcgaccgcg ataccaccag ccgcaaaggg gcgctggaac agcaactggc ggaagtgcat
     2221 cgcggcggcg cgaggatctt gattggcacg caaatgctgg cgaaaggtca ccactttccg
     2281 gatgtgacgc tggtggcgtt actggatgtg gacggcgcgc tgttttctgc cgattttcgc
     2341 tcggcagaac gtttcgccca gctttacacc caggtcgccg gtcgtgccgg gcgtgcgggt
     2401 aaacagggcg aagtggtgct gcaaacgcac catccggaac atcctctgtt gcaaacgttg
     2461 ctctataaag gctacgacgc ctttgccgaa caggcgctgg ctgagcgacg catgatgcag
     2521 ctgccgccgt ggaccagcca tgtgattgtg cgtgcggaag atcataacaa tcagcatgcg
     2581 ccgctgttcc tgcaacaact gcgtaatctg atcctttcca gcccgctggc agacgacaaa
     2641 ctgtgggttc tcggcccggt tccggctctg gcacctaagc gtggtggccg ctggcgctgg
     2701 cagatcttgt tgcagcaccc ttcccgcgtg cgcttgcaac acatcattag tggtacgctg
     2761 gcgcttatta atacaatacc ggattcccgc aaggtgaaat gggtgttgga tgtcgatccc
     2821 atcgaaggct aatgcgcaaa cgttgcgagg cggatcgaaa aattcaatat tcatcacact
     2881 tttcatgaaa attctgtaac cgttttcacg cgctatctgc taaaaatgtt gccgatgtga
     2941 agtaaacatg gatgtagtac gcctgacgtg ccaggcgagg agtgagtgtg aaagcgaaga
     3001 agcaggaaac tgccgcgacc atgaaagacg ttgccctcaa ggcaaaagtc tctacagcga
     3061 ctgtctcccg agcattaatg aatcccgata aagtctccca ggccacccgt aatcgggttg
     3121 aaaaagcggc ccgggaagtg ggttatttac cgcagcctat ggggcgcaac gtcaagcgta
     3181 atgaatcccg caccattctg gtgattgtcc cggatatctg cgatcccttc tttagcgaaa
     3241 ttattcgcgg tatcgaagtt acggcggcaa atcacggata tctggtgctg attggcgact
     3301 gtgcgcatca aaatcagcag gaaaaaacct ttatcgattt gatcatcacc aagcaaattg
     3361 atggcatgtt gctgctgggt tcaaggctgc cgtttgatgc cagcattgag gaacagcgta
     3421 atctgccgcc gatggtgatg gcgaacgaat tcgcaccgga actggagcta cctacagttc
     3481 atatcgacaa tctgaccgcc gcatttgatg cagtaaatta tctatatgag caagggcata
     3541 aacggattgg ctgtatagcc ggtcccgaag agatgccgct gtgtcactac cgcctgcaag
     3601 gttacgttca ggcgctgcgt cgctgcggca ttatggttga tccgcaatac atcgcccgtg
     3661 gcgacttcac cttcgaagcc ggaagcaaag cgatgcagca gctgcttgat cttccacaac
     3721 cgcctactgc tgtcttctgc catagcgatg tgatggcgct cggcgcactt tctcaggcaa
     3781 aacgccaggg gctgaaagtc ccggaagacc tttccataat cggttttgat aacatcgacc
     3841 tgacgcaatt ttgtgatccg ccgctgacaa ccatcgcgca gccgcgttac gaaatcggtc
     3901 gggaagctat gctgttattg cttgatcaaa tgcaggggca acacgttggc agtggctctc
     3961 gtttaatgga ctgcgaactt atcatccggg gatcaacacg cgcgttacct taaagtaaac
     4021 ctttaagact gccgtgactg gtcaaaggcc cgccgcttaa gtaacatggc gggctgacga
     4081 acgaataaat acagcgaaac gatagtggca caacgagatt atgtacgccg cagccaaccg
     4141 gcaccttcgc ggcgaaaaaa gagcacctca cggaaaaagc aacgaaatct gcctgcggtt
     4201 tctcccgcta tggtcgctat tgctgccgcc gttcttgtga cctttatcgg tggtctgtac
     4261 ttcattacgc atcacaagaa agaagagtcc gagacgctgc aaagccagaa agtgaccggt
     4321 aacggactac caccaaaacc agaagaacgc tggcgctaca ttaaagagct ggaaagtcgc
     4381 cagccgggtg tgcgtgcgcc cacagaacct tctgccggtg gtgaagtgaa aacgccggag
     4441 caactgacac cagaacaacg tcagcttctt gaacaaatgc aggctgatat gcgccagcag
     4501 ccaacgcagc tggttgaagt tccgtggaat gaacagacgc cggaacagcg tcagcaaacg
     4561 ctacaacgcc agcgtcaggc gcagcaatta gcggagcagc aacgcctggc tcagcagtcg
     4621 cgtacgactg aacaaagctg gcagcagcag acgcgtacgt cgcaagccgc gccggtacag
     4681 gcgcaaccgc gccagtctaa accggcttct actcagcagc cgtaccagga tctgctgcaa
     4741 acccctgcgc acacgaccgc gcagtcgaag ccgcagcaag ctgcgccggt tgctcgtgcc
     4801 gctgacgcgc caaaaccgac ggcggagaaa aaagacgaac gccgctggat ggtgcagtgc
     4861 ggttcgttca gaggcgcgga acaggcagag acggtacgtg ctcagctggc gttcgaagga
     4921 tttgactcga aaatcaccac caacaatggc tggaatcgtg tggtcattgg cccggtgaaa
     4981 ggcaaagaga acgcagacag caccctcaat cggttgaaga tggcgggtca tacaaactgc
     5041 attcggctcg ccgccggggg ttgaaaccct caaaatcccc cccatctata attgcattat
     5101 gccccgtact tttgtacggg gtttgtactc tgtattcgta accaaggggt cagctcgtga
     5161 caactatagt aagcgtacgc cgtaacggcc atgtggtcat cgctggtgat ggtcaggcca
     5221 cgttgggcaa taccgtaatg aaaggcaacg tgaaaaaggt ccgccgtctg tacaacgaca
     5281 aagtcatcgc gggctttgcg ggcggtactg cggatgcttt tacgctgttc gaactgtttg
     5341 aacgtaaact ggaaatgcat cagggccatc tggtcaaagc cgccgttgag ctagcgaaag
     5401 actggcgtac cgatcgcatg ctgcgcaaac ttgaagcact gctggcagtc gcggatgaaa
     5461 ctgcatcgct tatcatcacc ggtaacggtg acgtggtgca gccagaaaac gatcttattg
     5521 ctatcggctc cggcggccct tacgcccagg ctgcggcgcg tgcactgtta gaaaatactg
     5581 aactgagtgc tcgtgaaatt gctgaaaagg cgttggatat tgcaggcgac atttgcatct
     5641 ataccaacca tttccacacc atcgaagaat taagctacaa agcgtaagga tctcccatgt
     5701 ctgaaatgac cccacgcgaa atcgtcagcg aactggataa gcacatcatc ggccaggaca
     5761 acgccaagcg ttctgtggcg attgctctgc gtaaccgctg gcgtcgcatg cagctcaacg
     5821 aagagctgcg ccatgaagtg accccgaaaa atatcctgat gatcggcccg accggtgtcg
     5881 gtaaaactga aatcgcccgt cgtctggcta agctggcgaa tgcgccgttc atcaaagttg
     5941 aagcgaccaa attcaccgaa gtaggctacg tcggtaagga agtggattct attattcgcg
     6001 atctgaccga tgccgccgtg aaaatggtac gcgtccaggc tatcgagaaa aaccgttatc
     6061 gcgctgaaga actggcagaa gaacgtattc tcgacgtgct gatcccacct gctaaaaaca
     6121 actggggaca gaccgaacag cagcaggaac cgtccgctgc tcgtcaggca ttccgcaaaa
     6181 aactgcgtga aggccagctt gatgacaaag aaatcgagat cgatcttgcc gcagcaccga
     6241 tgggcgttga aattatggct cctccaggca tggaagagat gaccagccag ctgcagtcca
     6301 tgttccagaa cctgggcggc cagaagcaaa aagcgcgtaa gctgaaaatc aaagacgcca
     6361 tgaagctgct gattgaagaa gaagcggcga aactggtgaa cccggaagag ctgaagcaag
     6421 acgctatcga cgctgttgag cagcacggga tcgtgtttat cgacgaaatc gacaaaatct
     6481 gtaagcgcgg cgagtcttcc ggtccggatg tttctcgtga aggcgttcag cgtgacctgc
     6541 tgccgctggt agaaggttgc accgtttcca ccaaacacgg gatggtcaaa actgaccaca
     6601 ttctgtttat cgcttctggc gcgttccaga ttgcgaaacc gtctgaccta atcccggaac
     6661 tgcaaggtcg tctgccaatc cgcgttgaac tgcaggcact gaccaccagc gacttcgagc
     6721 gtattctgac cgagccgaat gcctctatca ccgtgcagta caaagcactg atggcgactg
     6781 aaggcgtaaa tatcgagttt accgactccg gtattaagcg catcgcggaa gcggcatggc
     6841 aggtgaacga atctaccgaa aacatcggtg ctcgtcgttt acacactgtt ctggagcgtt
     6901 taatggaaga gatttcctac gacgccagcg atttaagcgg tcaaaatatc actattgacg
     6961 cagattatgt gagcaaacat ctggatgcgt tggtggcaga tgaagatctg agccgtttta
     7021 tcctataatc gcgttcaatc attttcatca ttgtttgatg gggctgaaag gccccatttt
     7081 tattggcgcg tattatgact gaacaacaaa ttagccgaac tcaggcgtgg ctggaaagtt
     7141 tacgacctaa aaccctcccc ctcgcctttg ctgcaattat cgtcgggacg gcgctggcat
     7201 ggtggcaagg tcacttcgat ccgctggtcg ccctgctggc actaattacc gccgggctat
     7261 tacagatcct ttctaacctc gccaatgatt acggcgatgc ggtaaaaggc agcgataaac
     7321 ctgaccgcat tgggccgcta cgcggcatgc aaaaaggggt cattacccag caagagatga
     7381 aacgggcgct cattattacc gtcgtgctca tctgtctctc cgggttggca ctggttgcag
     7441 tggcatgcca tacgctggcc gattttgtcg gtttcctgat tcttggcggg ttgtcgatca
     7501 ttgccgctat cacctacacc gtgggcaatc gtccttatgg ttatatcggt ctgggtgata
     7561 tttccgtact ggttttcttt ggctggttga gtgtcatggg gagctggtat ttacaggctc
     7621 atacattgat tccggcacta atccttccgg cgaccgcatg cggcctgctg gcaacggcag
     7681 tactgaatat taataacctg cgtgatatca atagcgaccg cgaaaatggc aaaaacacgc
     7741 tggtggtgcg cttaggtgaa gtgaacgcgc gtcgttatca tgcctgcctg ctgatgggct
     7801 cgctggtgtg tctggcgctg tttaatctct tttcgctgca tagcctgtgg ggctggctgt
     7861 tcctgctggc ggcaccatta ctggtgaagc aagcccgtta tgtgatgcgg gaaatggacc
     7921 cggtggcgat gcgaccaatg ctggaacgta ctgtcaaggg agcgttactg actaacctgc
     7981 tgtttgtttt agggatattc ctaagccagt gggcagcata actgacaaat atcaattaac
     8041 aattgatgat tttgccaaca gcccacatag cgcgatatac tgaaaattct cgcagcaact
     8101 gaatgttaag cctatgaaat acgatacttc cgagctttgt gacatctatc aagaagatgt
     8161 taacgtcgtg gaaccgctgt tctccaactt tggcggacgg gcgtcgtttg gcggacaaat
     8221 aatcacggta aaatgtttcg aggacaacgg gttgctgtac gatctgctcg aacagaatgg
     8281 ccgtggtcgt gttcttgtcg tcgatggcgg tggttctgtt cgtcgcgcac tggtcgatgc
     8341 tgaactggcg cgtctggcag tacaaaatga atgggaaggt ctggtcattt acggcgcggt
     8401 gcgtcaggta gatgacctgg aagagttgga tatcggcatc caggcgatgg cggcaattcc
     8461 ggttggtgcc gctggcgaag gcattggcga aagcgatgtc cgcgtcaatt ttggcggtgt
     8521 caccttcttc tccggcgacc atctttatgc cgacaatacc gggattattc tttctgaaga
     8581 tccgctggat attgaatgat agaaaaggca ccgcaaggtg ccttttttct gcgttacctg
     8641 ttggcctaca cagtaaagaa attacgcgga agatgaagcg taatcagacc tcttccatgc
     8701 gacccagcag ggcctgcaga cgttcctgcc agccgttctg ctgttctttc agatggttgt
     8761 tctcacgctc cagctcttcg cgctgatgct gggcattttg aacttcctgc gacagtgagt
     8821 tgtttttttc tttcagctct tcgatttcca tctgcaacag agtgatggta tcaatcgcct
     8881 gctgtacttt tgcttccagt ttctcaaaca cttctaatga cattgtcata cctctcctga
     8941 attgcaaggc gttgatggat aaaaatcctc gtcccgatta ccggtgacgc cttaataaat
     9001 acgagcgcac tttagttagc tccgattgta tgaagccgcg ccatcgctgt ccagcggcac
     9061 gccttgcaga ttacggtttg ccacactttt catccttctc ctggtgacat aatccacatc
     9121 aatcgaaaat gttaataaat ttgttgcgcg aatgatctaa caaacatgca tcatgtacaa
     9181 tcagatggaa taaatggcgc gataacgctc attttatgac gaagcacaca cattttaagt
     9241 tcgatatttc tcgcttttgc tcgttaacga taagtttaca gcatgcctac aagcatcgtg
     9301 gaggtccgtg actttcacgc atacaacaaa cattaactct tcaggatccg attatgagtc
     9361 aaacatcaac cttgaaaggc cagtgcattg ctgaattcct cggtaccggg ttgttgattt
     9421 tcttcggtgt gggttgcgtt gcagcactaa aagtcgctgg tgcgtctttt ggtcagtggg
     9481 aaatcagtgt catttgggga ctgggggtgg caatggccat ctacctgacc gcaggggttt
     9541 ccggcgcgca tcttaatccc gctgttacca ttgcattgtg gctgtttgcc tgtttcgaca
     9601 agcgcaaagt tattcctttt atcgtttcac aagttgccgg cgctttctgt gctgcggctt
     9661 tagtttacgg gctttactac aatttatttt tcgacttcga gcagactcat cacattgttc
     9721 gcggcagcgt tgaaagtgtt gatctggctg gcactttctc tacttaccct aatcctcata
     9781 tcaattttgt gcaggctttc gcagttgaga tggtgattac cgctattctg atggggctga
     9841 tcctggcgtt aacggatgat ggcaacggtg taccacgcgg ccctttggct cccttgctga
     9901 ttggtctact gattgcggtc attggcgcat ctatgggccc attgacaggt tttgccatga
     9961 acccagcgcg tgacttcggt ccgaaagtct ttgcctggct ggcgggctgg ggcaatgtcg
    10021 cctttaccgg cggcagagac attccttact tcctggtgcc gcttttcggc cctatcgttg
    10081 gcgcgattgt aggtgcattt gcctaccgca aactgattgg tcgccatttg ccttgcgata
    10141 tctgtgttgt ggaagaaaag gaaaccacaa ctccttcaga acaaaaagct tcgctgtaat
    10201 atgactacgg gacaattaaa catgactgaa aaaaaatata tcgttgcgct cgaccagggc
    10261 accaccagct cccgcgcggt cgtaatggat cacgatgcca atatcattag cgtgtcgcag
    10321 cgcgaatttg agcaaatcta cccaaaacca ggttgggtag aacacgaccc aatggaaatc
    10381 tgggccaccc aaagctccac gctggtagaa gtgctggcga aagccgatat cagttccgat
    10441 caaattgcag ctatcggtat tacgaaccag cgtgaaacca ctattgtctg ggaaaaagaa
    10501 accggcaagc ctatctataa cgccattgtc tggcagtgcc gtcgtaccgc agaaatctgc
    10561 gagcatttaa aacgtgacgg tttagaagat tatatccgca gcaataccgg tctggtgatt
    10621 gacccgtact tttctggcac caaagtgaag tggatcctcg accatgtgga aggctctcgc
    10681 gagcgtgcac gtcgtggtga attgctgttt ggtacggttg atacgtggct tatctggaaa
    10741 atgactcagg gccgtgtcca tgtgaccgat tacaccaacg cctctcgtac catgttgttc
    10801 aacatccata ccctggactg ggacgacaaa atgctggaag tgctggatat tccgcgcgag
    10861 atgctgccag aagtgcgtcg ttcttccgaa gtatacggtc agactaacat tggcggcaaa
    10921 ggcggcacgc gtattccaat ctccgggatc gccggtgacc agcaggccgc gctgtttggt
    10981 cagttgtgcg tgaaagaagg gatggcgaag aacacctatg gcactggctg ctttatgctg
    11041 atgaacactg gcgagaaagc ggtgaaatca gaaaacggcc tgctgaccac catcgcctgc
    11101 ggcccgactg gcgaagtgaa ctatgcgttg gaaggtgcgg tgtttatggc aggcgcatcc
    11161 attcagtggc tgcgcgatga gatgaagttg attaacgacg cctacgattc cgaatatttc
    11221 gccaccaaag tgcaaaacac caatggtgtg tatgtggttc cggcatttac cgggctgggt
    11281 gcgccgtact gggacccgta tgcgcgcggg gcgattttcg gtctgactcg tggggtgaac
    11341 gctaaccaca ttatacgcgc gacgctggag tctattgctt atcagacgcg tgacgtgctg
    11401 gaagcgatgc aggccgactc tggtatccgt ctgcacgccc tgcgcgtgga tggtggcgca
    11461 gtagcaaaca atttcctgat gcagttccag tccgatattc tcggcacccg cgttgagcgc
    11521 ccggaagtgc gcgaagtcac cgcattgggt gcggcctatc tcgcaggcct ggcggttggc
    11581 ttctggcaga acctcgacga gctgcaagag aaagcggtga ttgagcgcga gttccgtcca
    11641 ggcatcgaaa ccactgagcg taattaccgt tacgcaggct ggaaaaaagc ggttaaacgc
    11701 gcgatggcgt gggaagaaca cgacgaataa tgtaaatgcc gaatgaagcg tttatgccgc
    11761 atccggtagt cccgaaacgt gcgggggcaa ccccgcacac atcaataatc cctcccttcc
    11821 cctgtgctac acttcgcgcc attccttact gcttagagtt tgctatgaga cgagaacttg
    11881 ccatcgaatt ttcccgcgtc accgaatcag cggcgctggc tggctacaaa tggttaggac
    11941 gcggcgataa aaacaccgcg gacggcgcgg cggtaaacgc catgcgtatt atgctcaacc
    12001 aggtcaacat tgacggcacc atcgtcattg gtgaaggtga aatcgacgaa gcaccgatgc
    12061 tctacattgg tgaaaaagtc ggtactggtc gcggcgacgc ggtagatatt gctgttgatc
    12121 cgattgaagg cacgcgcatg acggcgatgg gccaggctaa cgcgctggcg gtgctggcag
    12181 taggcgataa aggctgcttc ctcaatgcgc cggatatgta tatggagaag ctgattgtcg
    12241 ggccgggagc caaaggcacc attgatctga acctgccgct ggcggataac ctgcgcaatg
    12301 tagcggcggc gctcggcaaa ccgttgagcg aactgacggt aacgattctg gctaaaccac
    12361 gccacgatgc cgttatcgct gaaatgcagc aactcggcgt acgcgtattt gctattccgg
    12421 atggcgacgt tgcggcctca attctcacct gtatgccaga cagcgaagtt gacgtgctgt
    12481 acggtattgg tggcgcgccg gaaggcgtag tttctgcggc ggtgatccgc gcattagatg
    12541 gcgacatgaa cggtcgtctg ctggcgcgtc atgacgtcaa aggcgacaac gaagagaatc
    12601 gtcgcattgg cgagcaggag ctggcacgct gcaaagcgat gggcatcgaa gccggtaaag
    12661 tattgcgcct gggcgatatg gcgcgcagcg ataacgtcat cttctctgcc accggtatta
    12721 ccaaaggcga tctgctggaa ggcattagcc gcaaaggcaa tatcgcgact accgaaacgc
    12781 tgctgatccg cggcaagtca cgcaccattc gccgcattca gtccatccac tatctggatc
    12841 gcaaagaccc ggaaatgcag gtgcacatcc tctgattgat ttgatcgatt gagccttcca
    12901 gtccttcggg actggaattt ttttgttcgg agaacgaaga taaggcaagt caatcaaaac
    12961 aggagaaaaa catggctgat tgggtaacag gcaaagtcac taaagtgcag aactggaccg
    13021 acgccctgtt tagtctcacc gttcacgccc ccgtgcttcc gtttaccgcc gggcaattca
    13081 ccaagcttgg ccttgaaatc gacggcgaac gcgtccagcg cgcctactcc tatgttaact
    13141 cgcccgataa tcccgatctg gagttttacc tggtcaccgt ccccgatggc aaattaagcc
    13201 cacgattggc ggcactgaaa ccaggcgatg aagtgcaggt ggttagcgaa gcagctggct
    13261 tctttgttct ggatgaagta ccggactgcg aaacgctatg gatgctggca accggtacag
    13321 cgattggccc ttatttatcg attctgcaac taggcaaaga tttagatcgc ttcaaaaatc
    13381 tggtcctggt tcacgccgca cgttatgccg ccgacttaag ctatttgcca ctgatgcaag
    13441 aactggaaaa acgctacgaa gggaaactgc gcattcagac ggtggtcagt cgggaaacgg
    13501 cagcggggtc gctcaccggg cggataccgg cgttaattga aagtggggaa ctggaaagcg
    13561 cgattggcct gccgatgaat aaagaaacca gccatgtgat gctgtgcggc aatccacaga
    13621 tggtgcgcga tacacaacag ttgctgaaag agacccggca gatgacgaaa catttacgtc
    13681 gccgaccggg ccatatgaca gcggagcatt actggtaagc ggttacttat cgataaacgg
    13741 cacgatgagc aaatccgcac tcatcttatt gatcatcccg cgatatgccg gcatcaaacg
    13801 gttgataaat gagtgatgat gaccacagac aaggaggtcg cactgctctt tttgcataat
    13861 ttccagcagt gtttccggca tttctccgcg ttcaatacgc agttttgtct tcggccattg
    13921 aatatttttc gtcagtttat acagcttgtt atccgactta ttcttcaaca attgaagaat
    13981 atcttctgtt gcagggaagt agatacccgg atacaactcg cttaagccat catcaatatg
    14041 aattaacgtc aggtgagcgt cattatgtct ggcgagctcc agagctttat tcaccagtaa
    14101 ggcatcttct tcattcccgg aaattgccac gccaatgtgt ttataagcca tgtttaactc
    14161 cttctaaagc aactccatca agctaacgaa cgcagtgata actcaaaaat aatcatctcg
    14221 gcctggcatg agaaagtgaa ggccgcatca agctcaactt gcccctccag atccttaatc
    14281 ttgtaggcca cgctggcccc ttcatctgca gaggatgcag cggcggcaag ctctttcagt
    14341 ttggtcataa aagactctgc ttcttcgcgg gtggcaaaaa agcgcgaaaa cttactggta
    14401 cagttgtcgt tatcaatcac cgtaccgata tcaatcgcac atcctttagt actgcattta
    14461 tctacgacat ctttcatagg gcacctctgt atattcgcct ctctgtcgat tacgacttaa
    14521 gaataatgcg ccgttaaggg aagaacaaac aagtaatgat cttatggatt accatttttt
    14581 ttgatattgc ttaaggtgaa gagttcagaa ggattttccg ggtattgtca tttgcatcgt
    14641 ggaaataaca atcccggaaa aaaatagcag taagagatat attaaaggtt aattacttga
    14701 tttattgtcg gctttatact tcacatcctg agtatcttta ccatatttat tttcgccttg
    14761 tgtgccaaca aacgcaccaa gatcgataag catcatcacc agaatcaacg tcgggacaaa
    14821 acgccccacc gcccattgcc agacacccgg taaaatcgcc cagttacccg ccagcagcat
    14881 ccacgccaca atcatcagaa atgcccatgc gccggaacgc ccgcgatcat gcaagcgctt
    14941 aacagttact gccgctgttg gccaaagcaa gcacacaagg caaaacgccg cggtctgaat
    15001 atcgagtaaa tttttacccg ccagtgagaa aagcaccagc atgcctgcga accacaggcc
    15061 tatccaaatc cagaaatcac ggcgtccaat acgcccttta aatgagaata accattgctg
    15121 tatggtcatg taagttcctt gatggttgtc ttttccagga ttctaccctt ttgacaaggg
    15181 cgacagatat cgttttaatc ggagccagtc ataacaaaag gtactgtcaa tgaagccagg
    15241 gtgtacgctg ttttttctct tatgttctgc attaaccgtt acaacaacgg cgcatgcaca
    15301 aacaccagat acggcaacga ccgcgcctta tctgctggct ggagccccta ctttcgatct
    15361 ctccatcagc cagtttcgag aagactttaa cagccagaat cctagcctgc cactgaacga
    15421 atttcgtgcc atcgacagca gtcccgacaa agccaatctc actcgtgctg caagtaaaat
    15481 taacgagaac ttgtatgctt ctacagcgct ggagcgcggt accttaaaaa tcaaaagcat
    15541 tcaaatgacc tggctaccca tccaggggcc agagcaaaaa gcagcgaaag cgaaagctca
    15601 ggaatacatg gcagcagtga tccgcacact caccccatta atgaccaaaa cacaaagcca
    15661 gaaaaaactg cagtcgctac taacggcggg gaaaaacaaa cgttattaca ccgagacaga
    15721 aggtgcactg cgttatgttg tcgcggacaa cggcgaaaag gggctgacct tcgctgttga
    15781 accgattaag ctggcgctat ctgaatcgct tgaaggtttg aataaatgac aaaaagcaaa
    15841 gcctttgtgc cgatgaatct ctatactgtt tcacagacct gctgccctgc ggggcggcca
    15901 tcttccttta ttcgcttata agcgtggaga attaaaatgc gacatccttt agtgatgggt
    15961 aactggaaac tgaacggcag ccgccacatg gttcacgagc tggtttctaa cctgcgtaaa
    16021 gagctggcag gtgttgctgg ctgtgcggtt gcaatcgcac caccggaaat gtacatcgat
    16081 atggcgaagc gcgaagctga aggcagccac atcatgctgg gtgcgcaaaa cgtggacctg
    16141 aacctgtccg gcgcattcac cggtgaaact tctgccgcta tgctgaaaga catcggcgct
    16201 cagtacatca tcatcggcca ctctgaacgt cgtacttacc acaaagagtc tgacgaactg
    16261 attgcgaaaa aattcgcggt gctgaaagag cagggcctga ctccggttct gtgcatcggt
    16321 gaaaccgaag ctgaaaacga agcgggcaaa actgaagaag tttgcgcacg tcagatcgac
    16381 gcggtactga aaactcaggg tgctgcggca ttcgaaggtg cggttatcgc ttacgaacct
    16441 gtatgggcaa tcggtactgg caaatctgca actccggctc aggcacaggc tgttcacaaa
    16501 ttcatccgtg accacatcgc taaagttgac gctaacatcg ctgaacaagt gatcattcag
    16561 tacggcggct ctgtaaacgc gtctaacgct gcagaactgt ttgctcagcc agatatcgac
    16621 ggcgcgctgg ttggcggtgc ttctctgaaa gctgacgctt tcgcagtgat cgttaaagct
    16681 gcagaagcgg ctaaacaggc ttaagtctga caggtgccgg atttcatatc cggcacttac
    16741 tttccttaac tcctcgcctt aacgcaaaat ctcacactca tgatcctgga tttcctcggc
    16801 tgaagcacgg ttcagcgtca gtagatttcg ttgtgtcgcc agcaacacaa acgaattatc
    16861 actctgccgt accatcgcca gcccgtagct tcccatatgt tcccgcgcct caggcacttc
    16921 ttctgcaagc atcataaatg ggctgcgttg taccagttcg ctttccgtta cccgacgcgc
    16981 caggtattca tgcccgcgca aaccacctgg cagtggcaac cagcggctgc tgatgttcgc
    17041 cagattttta tccagttgtt cgcgcacatc aggacgaata caagagatat gaatatgaaa
    17101 atggttttgc gtacgcccgg tgcgggagtt gatcgccaaa gaaaccgcgc gatcgggaac
    17161 cggctggccg tattttttgc tcataaaatc acgcgcctgc caggccaacc aaaagaagtt
    17221 cggcgttgaa ggatcggtca acaaaggact ttcagtaccg ttaatacgat acgttggcat
    17281 caacagatat tgcagtggac cattaagatc ttttaaaacc acgtatccgg cattgggttt
    17341 gacttccgca catggcgaag gattttgatt ttgctgctga ttgggcaaac attcctcaag
    17401 gacaatctta cgtaatgtat ccgactcttc accggttaat ttccagtaac caataccggc
    17461 agccacaacg gcgataacta tcatcaccaa aaaaagaaga cccgcttttt tcatcttttt
    17521 ttccctgtac ctcaaagaga tgcaagggta acgcaaaatc gtgacaaata aaaaacccgg
    17581 tcgcgagaaa ccaccgggtt tgataattat cctggggaaa tcagcgtttg ctgatctgat
    17641 cgaacgtacc gccgttagca aaatgctctt tttgcgcttt cgtccagccg ccgaactctt
    17701 catcaatggt gaataacttc agctttggaa acgcattttc gtactttttc gctacttcag
    17761 cgtcgcgcgg acggtagtag tttttcgcgg caatttcctg accttctggc gagtagagat
    17821 atttcaggta ggcttccgcc acctctttgg tgcctttttt ctcgaccact ttatcgacca
    17881 ccgacacggt tggctctgcg aggatagact cactcggcgt gacgatttcg aatttatctt
    17941 tccccagttc attcgctacc agcagagctt cgttttcccg ggcgatcagt acatcgccaa
    18001 tcccgcgctc gacaaaggtg ttagtggagc cgcgcgcgcc agaatccaga acttcgacgt
    18061 ttttatacag tgcccgaaca aaatcctgtg cttttgcctg atcgttgttg ttgtgatgca
    18121 gcgcgtagcc ccaggcagcc aggtagttcc agcgtgcgcc accagagctt ttcggattag
    18181 gcgtgatcac cgaaacaccc ggtttaatca gatcgttcca gtcatggatc tgcttcggat
    18241 tgcccttacg caccaggaaa acaatggtgg aagtgtacgg tgcggagtta tccggcagac
    18301 gtttgatcca ctctttatca atccgcccgc gttccgcaat cgcgtccacg tcataggcca
    18361 gagccagcgt gacaacatca gcttcaatac cgttgattac cgacgtcgct tgtttacctg
    18421 aaccaccgtg tgactgacga atcaccacgt tatcaccagt ttgctgtttc cagtgggcgc
    18481 tgaatgcctt gttgtactgt tcgtacaatt cgcgcgttgg atcatatgaa acgttaagaa
    18541 gctgaatatc ctttgccata acgttggttg ccgccagcaa aaatgttaac cctacgcccc
    18601 acttgttcat cgcccgactc tcttatgttg tgttgtgatg agcaaagcgt gccagaaggt
    18661 taaccaaaca ttaaagaata aaaaaagatt ggctataact tgcgggtata tgttgggggt
    18721 taaaaaggcg gaagaaatcc gcctcatatt gctgacaaag tgcgctttgt ccatgccgga
    18781 tgcggcgcga acgccttatc cggcctacaa aagtttgcaa attcaataaa ttgcagaatt
    18841 catgtaggcc tgataagcga agcgcatcag gcatttttgc ttctgtcatc ggtttcaggg
    18901 taaaggaatc tgcctttttc cgaaatcatt aatacagttt tttcgcgcag tccagccagt
    18961 cacctttgaa cggacgcttc atgttttcga tagcgtcgat gatgtcgtgg tgaaccagct
    19021 gttcgttctg gatacctaca caacgaccgc cgtaacctgc cagcagcaga tcgatagcgt
    19081 aagcgcccat acgggaagcc agaatacggt cgtaaggcac cggagaacca ccgcgctgga
    19141 tgtggcccag cacagttgcg cgggtttcac gaccggtttc tttctcgatg aaatgcgcca
    19201 gttcgtcaac atcacacata tgttcggtaa tcgccacgat cgcgtgtttt ttacctttcg
    19261 cgatacccgc tttgatttcg tttaccaggt cttcacggct gaattcaact tccggaacca
    19321 caacgaattc acagccaccg gcaatggccg cagccaacgt cagatcgcca caataacggc
    19381 ccatcacttc caccacggaa atacgctggt gagaagaaga ggtgtcacgc agacggtcga
    19441 tcgcttctac aacggtgctc agcgcagtga agaaaccgat agtgtagtca gtgcctttga
    19501 tgtcgttgtc gatagtgccc ggcagaccga tgcacgggaa gcccatttcg gtcagacgca
    19561 ttgcacccat gtaggaaccg tcaccgccga taaccaccag cgcgtcgatc ccacgttttt
    19621 tcaggttttc gatagccacg gcgcggatgt tctcgtcgcg gaattccggg aagcgcgcag
    19681 aaccgaggaa cgtaccgcca cggttgatca tgtcagaaac gctgtaacgg tctagctgta
    19741 ccatacggtc ttcatacaga cccagatagc cgtcataaat gcccattact tccagacctt
    19801 ctgtcagcgc agaacgaaca accccgcgaa ttgcggcgtt catgcctggc gcatcaccgc
    19861 cgcttgtcaa cacaccgatt ttcttaatca tgactacctc tgaactttgg aatgcaaaat
    19921 aaaatctgtt gccggaagtc ttcttgcaca tcgaagtgat ccaacgaatg tgcaaatagt
    19981 ataacaatca cttcctgctg aattgattca ggtcaggcca aatggcggta ttttatacac
    20041 aaaatgcggg tctggctctc ttttatactg attatgaaag catagaccgt ttaccctccc
    20101 tgggtacgac ggaacagggg tcctgatgga taattacatc cgatcccgga aaacgccgta
    20161 aaatagcctg ctctacctga tccgccacca tatgtgcctg aaccaaaggc agagagtctt
    20221 ccatttccaa atgaatctga ataaagcggg tcggccctga ctgccgcgtg cgaagatcgt
    20281 gagcgccgct aacacccggc caggaagtca cgatatcaat aatttcttgc cgttcctcat
    20341 caggcaatgc gcgatccagt aatgactgta ccgcctcata tcccatgcgt aacgcgctat
    20401 ataaaatata gatgccgatt cccaatgcaa acagagcatc ggcgcgatgc cagccgtacc
    20461 aggacaaccc cagcgccagc agaattgcgc cgttcatcat aacatcagac tggtaatgta
    20521 gcatatcagc ccgcaccgcc tggctttgcg tccggcgcac cacccaacgc tgaaacgaga
    20581 caaggataat cgtacaaatt agcgccacaa ttgtcacgat aaccccgacg cctggatctg
    20641 tcatcggtgt tggagatatc agatgttgaa tacccgtcaa aaacaggaat agtgccgaac
    20701 cggagataaa catactttgc gccagcgccg cgagggactc tgctttaccg tgaccaaacg
    20761 agtgattatc gtcggcaggt tgcagggaat atcgcaccac cagtaaattc gtcaacgacg
    20821 cgccgatatc caccagcgaa tccaccagcg cggcgagaat actcaccgac ccggtatacc
    20881 accatgcaaa aattttaatc agcaatagca acgaagccat cgccgtcgca gcaatcgccg
    20941 cccgactgac cagccgtcca taagattgat tcataaatac tcccgctatc aactgacgct
    21001 agtataacgg aagcaaatca tctgcaatgc attaagcagc aggcaaattg aggataaaaa
    21061 aaacccccac atcatgtggg ggaagacagg gatggtgtct atggcaagga aaacagggtt
    21121 tactactggg aacgtgagtt gctactactc aatagcttca acgatgaact tttttgccat
    21181 tgcgtcacgt cacgcaactg ctccattcgt tgttgatgtt tctcgtttaa aaccgcttgc
    21241 tgctccggcg ttaacaggcg atacatttgg ttgcggactt ttgccatctc aacctgacga
    21301 gcaatttgct cattcgccat tttttctgcc tgtgcgcgca cagcgttttc atcaaaattt
    21361 tctgcggtga caaggcgatg cattgtctcc agttcgctaa cattaacagg aggctgttcg
    21421 tgccgggcct gttgcataag atctcgcatc tgctgacgct gatgttcggt taaacttatg
    21481 ccgtcgaaca tatggctctg cgtactgcgc tgcgtaagtt cttcacccgg atgccagtta
    21541 tcgcctgaac cgacttcagc agcgtggctt aatgaactga ctgccagcgt tgaggccatg
    21601 acggcagcgg taactatgcg catcatttgc tcccaaaatc tttctgtcgc gattcaacga
    21661 tagagagttt acgattcagg ctgcaaacat gcgtcagggg gtgtaaaaca acgtaaagtc
    21721 atggattagc gacgtctgat gacgtaattt ctgcctcgga ggtatttaaa caatgaataa
    21781 aatcctgtta gttgatgatg accgagagct gacttcccta ttaaaggagc tgctcgagat
    21841 ggaaggcttc aacgtgattg ttgcccacga tggggaacag gcgcttgatc ttctggacga
    21901 cagcattgat ttacttttgc ttgacgtaat gatgccgaag aaaaatggta tcgacacatt
    21961 aaaagcactt cgccagacac accagacgcc tgtcattatg ttgacggcgc gcggcagtga
    22021 acttgatcgc gttctcggcc ttgagctggg cgcagatgac tatctcccga aaccgtttaa
    22081 tgatcgtgag ctggtggcac gtattcgcgc gatcctgcgc cgttcgcact ggagcgagca
    22141 acagcaaaac aacgacaacg gttcaccgac actggaagtt gatgccttag tgctgaatcc
    22201 aggccgtcag gaagccagct tcgacgggca aacgctggag ttaaccggta ctgagtttac
    22261 cctgctctat ttgctggcac agcatctggg tcaggtggtt tcccgtgaac atttaagcca
    22321 ggaagtgttg ggcaaacgcc tgacgccttt cgaccgcgct attgatatgc acatttccaa
    22381 cctgcgtcgt aaactgccgg atcgtaaaga tggtcacccg tggtttaaaa ccttgcgtgg
    22441 tcgcggctat ctgatggttt ctgcttcatg ataggcagct taaccgcgcg catcttcgcc
    22501 atcttctggc tgacgctggc gctggtgttg atgttggttt tgatgttacc caagctcgat
    22561 tcacgccaga tgaccgagct tctggatagc gaacagcgtc aggggctgat gattgagcag
    22621 catgtcgaag cggagctggc gaacgatccg cccaacgatt taatgtggtg gcggcgtctg
    22681 ttccgggcga ttgataagtg ggcaccgcca ggacagcgtt tgttattggt gaccaccgaa
    22741 ggccgcgtga tcggcgctga acgcagcgaa atgcagatca ttcgtaactt tattggtcag
    22801 gccgataacg ccgatcatcc gcagaagaaa aagtatggcc gcgtggaact ggtcggtccg
    22861 ttctccgtgc gtgatggcga agataattac caactttatc tgattcgtcc ggccagcagt
    22921 tctcaatccg atttcattaa cttactgttt gaccgcccgc tattactgct gattgtcacc
    22981 atgttggtca gtacgccgct gctgttgtgg ttggcctgga gtctggcaaa accggcgcgt
    23041 aagctgaaaa acgctgccga tgaagttgcc cagggaaact tacgccagca cccggaactg
    23101 gaagcggggc cacaggaatt ccttgccgca ggtgccagtt ttaaccagat ggtcaccgcg
    23161 ctggagcgca tgatgacctc tcagcagcgt ctgctttctg atatctctca cgagctgcgc
    23221 accccgctga cgcgtctgca actgggtacg gcgttactgc gccgtcgtag cggtgaaagc
    23281 aaggaactcg agcgtattga aaccgaagcg caacgtctgg acagcatgat caacgatctg
    23341 ttggtgatgt cacgtaatca gcaaaaaaac gcgctggtta gcgaaaccat caaagccaac
    23401 cagttgtgga gtgaagtgct ggataacgcg gcgttcgaag ccgagcaaat gggcaagtcg
    23461 ttgacagtta acttcccgcc tgggccgtgg ccgctgtacg gcaatccgaa cgccctggaa
    23521 agtgcgctgg aaaacattgt tcgtaatgct ctgcgttatt cccatacgaa gattgaagtg
    23581 ggctttgcgg tagataaaga cggtatcacc attacggtgg acgacgatgg tcctggcgtt
    23641 agcccggaag atcgcgaaca gattttccgt ccgttctatc ggaccgatga agcacgcgat
    23701 cgtgaatctg gcggtacagg attggggctg gcgattgttg aaaccgccat tcagcagcat
    23761 cgtggctggg tgaaggcaga agacagcccg ctgggcggtt tacggctggt gatttggttg
    23821 ccgctgtata agcggagtta aactccgcat ttgtaggcag gataaggcgt ttacgccgca
    23881 tccggcattt gagcaggata aggcgtttac gccgcatccg gcatttgagc aggatgcctg
    23941 atgcgacgct gatagcgtct tatcaggcct acactccggg ggttattttc cccacaaccg
    24001 gcgggaaaaa tcttcgatct tgccgctcag tcggcgcttt tgcatcgtcc gcgtccagct
    24061 tttcgataac ccggcggcgg agagtaaacg gtgatactga tcgtcatcaa acggcatatg
    24121 ccatgcgatg gcggcagcct cctgcacggt cacatcgctg acacgggaaa ccaactccag
    24181 cggcgcgtcc gcagacactt tccctggcgc tatcacgcta tacaaccacc ccaccttacc
    24241 tgtgttttgc atcaactgcg ctatatcgct gatatcaaaa tggtaattga gtttatagca
    24301 aggcgagcgc ggctggctga cctgaattaa tgcctctccc cagcggaaaa tatcgcccat
    24361 atgaacatta ctttccgtga gaccgtcggt tgagagattt tcgccaaacg caggcgcaac
    24421 aaacaactcc gcctgttccg gaaattcccg cacccagtag agatagtgct cacgaggata
    24481 atgacacagc gctctgtctg gcccgccgtg aacctttttc tccgcctgct cgtcaccttc
    24541 cagccccagc tccgtcagca tcaactcacc atcaacctgg attttggcaa ttgcgctggg
    24601 tttgccttcg ggataagcct gaatcttgcc tgtgtataca tcaaccggat atcgcatctg
    24661 ttccctctcc acgagaaatt tccccattag accatattta ttttatgtca ggcgtgctgt
    24721 tttcgcggta agtatccagg caggaactca atagcaaacc ggacgaacgt tagttcatcc
    24781 ggctgtgggg attaaggaag caggtttatt tcaccgtacc taaaatttcg attttcgctg
    24841 ctctggcttt gactttacgt gaccagatag aggtaagaat tggcaccaga atcgaagtca
    24901 caatgaccgc cgtcgctacc agcgaagttg ctgccggagc catcggttta aacgcaggca
    24961 ccatttctgc aatcagcaca ggtgtcgcta ccgcggcccc tgcggaactg gaagcggcaa
    25021 ttccggcagt gccatcgcca ccgccaatca atttatcggc gataatcaac ggaataccgg
    25081 tcacgataat tactgccaca cccaacagga tccccagcaa accagtctgg gcaatcacag
    25141 tcaaatcaat ggtattgccc agcgcgaagg caaagaatgg aatcagcgtt tgcaccgctt
    25201 tgctgaaaaa ttctcgtaat tcagggtcaa ggttccccag ggcaaagccc accaggaacg
    25261 gtaatactgc gccgacgaaa acatgcggtt caaacgaggc aatccctgca gtgcccagaa
    25321 taatcatcgt catgagcggc ccggactcca acgacatcaa cacaaatgcc ccagcttctt
    25381 cttttgtgcc gtactgctgc atgatggaag cgtaaagtcc gccattggtc atatccatcg
    25441 ccgccaccag cgccagcgtt gaaagtccgg caaagaatcc aacttcaaca ccatgttccg
    25501 gaataatgcg cgaggcaatt gccgcaacca cccacgcgac ggcaattttt gttaccacca
    25561 gcgtaccgga tttacgcaat accgttcccg tcgcgcttaa ttttattgac gcccccatgc
    25621 aaaaaaacca caccgccaga atgggcaccg taccggtaat catcccgttg gtgaatgatc
    25681 caaaatattt ccccgccccc ggcgagaagg tgtggcacag tgcgccaagg aatagcggga
    25741 cgagcatcat cccccccggg attttctcaa tcgagcgttt tatctgcatt tccatcacct
    25801 gtcggcatat ttttaagggc tgatgcgtca ttcctcaatg gagcatcagt gttgatggaa
    25861 acgaagatag acattcattt aatcaataaa agtgatcaaa accacatcaa ttgaaacgct
    25921 gttttatttt tatcggatca ttgtttttat tttgaaattg atcacaaaaa aaccaccgcc
    25981 gttggcgatg gttcattttt taagctgata tgcggcctat acgcctcatt gcagcaggcg
    26041 gcaaatgatt attttttcgc cgcaaaacgt gccgctgctt cgtcccagtt caccacgttc
    26101 cagaactctt taatgtagtc cggacggcgg ttctggaatt tcaggtagta agcatgttcc
    26161 cacacatcca ggcccagaat cgggaagccg gaagcgccag aaatagcttc acccatcagc
    26221 ggagaatcct ggttagcagt agaaaccacc gccagtttat cgcctttcag caccagccat
    26281 gcccagccgg aaccaaagcg ggaagctgcc gctttttcaa attctgcttt gaagttatca
    26341 acggagccga agtcacgttc gatagccgct ttcaggtcac cctgcagggt ggtgcctttt
    26401 ttcagacctt tccagaacag gctgtggtta gcgtgaccgc cagcgttgtt gcgcagtacg
    26461 gttttcttgt ctgctggcag ctggtccagt ttggtgatca gctcttcaac cggcaggttg
    26521 gcaaattctg gcaggctttc cagcgccgcg ttggcgttgt ttacgtaggt ctgatggtgt
    26581 ttggtgtggt ggatttccat ggtctgctta tcgaagtgcg gttccagggc atcgtaagca
    26641 tacggcaggg atggcagggt atagctcata ttcatctcca gtattgtcgg gcggccgatt
    26701 gttaatgccg cgtaagcagt tggttcatta tagttaatta aatgatattg aaaatgatta
    26761 tcaatgccgt acttttcgta agggtatggt tttgcaggaa aatgcccgag atgtgaagca
    26821 aatcacccac ttaatgccgt gattgccagt aaatcgacaa cggcggcaac aggcgaaagg
    26881 ttaatcgaca gcacgatttt tacactcatc tcgtcggaga tgtgaagcga cgaaaaatga
    26941 tgaggataag aagatgagta acgcgattac gatggggata ttttggcatt tgatcggcgc
    27001 ggccagtgca gcctgttttt acgctccgtt caaaaaagta aaaaaatggt catgggaaac
    27061 catgtggtca gtcggtggga ttgtttcgtg gattattctg ccgtgggcca tcagcgccct
    27121 gttactaccg gatttctggg cgtattacag ctcgtttagt ctctctacgc tgctgcctgt
    27181 ttttctgttc ggcgctatgt gggggatcgg taatatcaac tacggcctga ccatgcgtta
    27241 tctcggcatg tcgatgggaa ttggcatcgc cattggcatt acgttgattg tcggtacact
    27301 gatgacgcca attatcaacg gcaatttcga tgtgctgatt aacaccgaag gcggacgcat
    27361 gacgttgctc ggcgttctgg tggcgctgat tggcgtaggg attgtaactc gagccgggca
    27421 gttgaaagag cgcaagatgg gcattaaagc cgaagagttc aatctgaaaa aagggctggt
    27481 gctggcggtt atgtgcggca ttttctctgc cgggatgtcc tttgcgatga acgccgcaaa
    27541 accgatgcat gaagccgctg ccgcacttgg cgtcgatcca ctgtatgtcg ctctgccaag
    27601 ctatgttgtc atcatgggcg gcggcgcgat cattaacctc ggtttctgtt ttattcgtct
    27661 ggcaaaagtg aaggatttgt cgctaaaagc cgacttctcg ctggcaaaac cgctgatcat
    27721 tcacaatgtg ttactctcgg cactgggcgg gttgatgtgg tatctgcaat tctttttcta
    27781 tgcctggggc cacgcccgca ttccggcgca gtatgactac atcagttgga tgctgcatat
    27841 gagtttctat gtattgtgcg gcggtatcgt cggactggtg ctgaaagagt ggagcaatgc
    27901 aggacgccgt ccggtaacgg tgttgagcct cggttgtgtg gtgattattg tcgccgctaa
    27961 catcgtcggc atcggcatgg cgaattaatc tttctgcgaa ttgagatgac gccactggct
    28021 gggcgtcatc ccggtttccc gggtaaacac caccgaaaaa tagttactat cttcaaagcc
    28081 acattcggtc gaaatatcac tgattaacag gcggctatgc tggagaagat attgcgcatg
    28141 acacactctg acctgtcgca gatattgatt gatggtcatt ccagtctgct ggcgaaattg
    28201 ctgacgcaaa acgcgctcac tgcacgatgc ctcatcacaa aatttatcca gcgcaaaggg
    28261 acttttcagg ctagccgcca gccgggtaat cagcttatcc agcaacgttt cgctggatgt
    28321 tggcggcaac gaatcactgg tgtaacgatg gcgattcagc aacatcgcca actgcccgaa
    28381 cagcaactct gccatttcgt tagcaaacgg cacatgctga ctactttcat gctcaagctg
    28441 accgataacc tgccgcgcct gcgccatccc cacgctacct aagcgccagt gtggttgccc
    28501 tgcgctggcg ctaaatcccg gaatcgcccc ctgccagtca agattcagct tcagccgctc
    28561 cgggcaataa ataatattct gcaaaaccag atcgttaacg gaagcgtagg agtgtttatc
    28621 gtcagcatga atgtaaaaga gatcgccacg ggtaatgcga taagggcgat cgttgagtac
    28681 atgcaggcca ttaccgcgcc agacaatcac cagctcacaa aaatcatgtg tatgttcagc
    28741 aaagacatgt tgcggataac ggtcagccac agcgactgcc tgctggtcgc tggcaaaaaa
    28801 atcatctttg agaagtttta actgatgcgc caccgtggct acctcggcca gagaacgaag
    28861 ttgattattc gcaatatggc gtacaaatac gttgagaaga ttcgcgttat tgcagaaaac
    28921 catcccgtcc ctgacgaata tcacgcggtg accagttaaa ctctcggcga aaaagcgtcg
    28981 aaaagtggtt actgtcgctg aatccgcaat gataggcgat gtcagtaacg ctggcctcac
    29041 tgtggcgtag cagatgtcgg gccttcatca gtcgcaggcg gttcaggtaa cgctgaggcg
    29101 tcagtcccgt ttgctgctta agctgccgat gtagcgttcg cagtgaaaga gaaaattgat
    29161 ccgccacggc atcccaattc acctcatcgg caaaatggtc ctccagccag gccagaagca
    29221 agttgagacg tgatgcgctg ttttccaggt tcttctgcaa actgctttta cgcagcaaga
    29281 gcagtaattg cataaacaaa gatctcgcga ctggcggtcg agggtaaatc attttcccct
    29341 tcctgctgtt ccatctgtgc aaccagctgt cgcacctgct gcaatacgct gtggttaacg
    29401 cgccagtgag acggatactg cccatccagc tcttgtggca gcaactgatt cagcccggcg
    29461 agaaactgaa atcgatccgg cgagcgatac agcacattgg tcagacacag attatcggta
    29521 tgttcataca gatgccgatc atgatcgcgt acgaaacaga ccgtgccacc ggtgatggta
    29581 tagggctgcc cattaaacac atgaataccc gtgccatgtt cgacaatcac aatttcatga
    29641 aaatcatgat gatgttcagg aaaatccgcc tgagggagcc ggggttctat cgccacggac
    29701 gcgttaccag acggaaaaaa atccacacta tgtaatacgg tcatactggc ctcctgatgt
    29761 cgtcaacacg gcgaaatagt aatcacgagg tcaggttctt accttaaatt ttcgacggaa
    29821 aaccacgtaa aaaacgtcga tttttcaaga tacagcgtga attttcagga aatgcggtga
    29881 gcatcacatc accacaatga agcaaattgt gaacatcatc acgttcatct ttccctggtt
    29941 gccaatggcc cattttcctg tcagtaacga gaaggtcacg aattcaggcg ctttttagac
    30001 tggtcgtaat gaaattcagc aggatcacat tatgaccttt cgcaattgtg tcgccgtcaa
    30061 tctcggcgca tccagtgggc gcgtgatgct ggcgcgttac gagcgtgaat gccgcagcct
    30121 gacgctgcgc gaaatccatc gttttaaaaa tgggctgcat agccagaacg gttatgtcac
    30181 ctgggatgtg gatagcctgg aaagtgccat tcgccttggc ttaaacaaag tgtgtgagga
    30241 agggattcgt atcgatagca ttgggattga tacctggggc gtggactttg tgctgctcga
    30301 ccaacagggt cagcgtgtgg gcctgcccgt tgcttatcgc gatagccgca ccaatggcct
    30361 aatggcgcag gcacagcaac aactcggcaa acgcgatatt tatcaacgta gcggcatcca
    30421 gtttctgccc ttcaatacgc tttatcagtt gcgtgcgctg acggagcaac aacctgaact
    30481 tattccacac attgctcacg ctctgctgat gccggattac ttcagttatc gcctgaccgg
    30541 caagatgaac tgggagtaca ccaatgccac caccacacaa ctggtcaata tcaatagcga
    30601 cgactgggac gagtcgctac tggcgtggag cggggccaac aaagcctggt ttggtcgccc
    30661 gacgcatccg ggtaatgtca ttgggcactg gatttgcccg cagggtaatg agattccggt
    30721 ggtcgccgtt gccagccatg ataccgccag cgcggttatc gcctcgccgt taaacggttc
    30781 acgcgccgct tatctctctt ctggcacctg gtcattgatg ggcttcgaaa gccagacgcc
    30841 atttaccaat gacacggcgc tggcagccaa catcaccaat gaaggcgggg cggaaggtcg
    30901 ctatcgggtg ctgaaaaata ttatgggctt atggctgctt cagcgagtgc ttcaggaacg
    30961 gcaaatcaac gatctcccgg cgcttatcgc cgcgacacag gcacttccgg cctgccgctt
    31021 catcatcaat cccaatgacg atcgctttat taatcctgag gcgatgtgca gcgaaattca
    31081 ggctgcgtgt cgggaaacgg cgcaaccgat cccggaaagt gatgctgaac tggcgcgctg
    31141 tattttcgac agtctggcgc tgctgtatgc cgatgtgttg catgagctgg cgcagctgcg
    31201 cggtgaagat ttctcgcaac tgcatattgt cggcggcggc tgccagaaca cgctgctcaa
    31261 ccagctatgc gccgatgcct gcggtattcg ggtgatcgcc gggcctgttg aagcctcaac
    31321 gctcggcaat atcggcatcc agttaatgac gctggatgaa ctcaacaatg tggataattt
    31381 ccgtcaggtc gtcagcacca ccgcgaatct gaccaccttt acccctaatc ctgacagtga
    31441 aattgcccac tatgtggcgc agattcactc tacacgacag acaaaggagc tttgcgcatg
    31501 accactcaac tggaacaggc ctgggagctg gcgaaacagc gtttcgcggc ggtggggatt
    31561 gatgtcgagg aggcgctgcg ccaacttgat cgtttacccg tttcaatgca ctgctggcag
    31621 ggcgatgatg tttccggttt tgaaaacccg gaaggttcgc tgaccggtgg gattcaagct
    31681 actggtaatt atccgggcaa agcgcgtaat gccagtgagc tacgtaccga tctggaacag
    31741 gctatgcggc tgattccggg gccgaaacgg cttaatttac atgccatcta tctggaatca
    31801 gacacgccag tctcgcgcga ccagatcaaa cctgagcact tcaaaaactg ggttgaatgg
    31861 gcgaaagcca atcagctcgg tctggatttt aacccctcct gtttttcgca tccgctaagc
    31921 gccgatggct ttacgctttc ccatgccgac gacagcattc gccagttctg gattgatcac
    31981 tgcaaagcca gccgtcgcgt ttcggcctat tttggcgagc aactcggcac accatcggtg
    32041 atgaacatct ggatcccgga tggtatgaaa gatatcaccg ttgaccgtct cgccccgcgt
    32101 cagcgtctgc tggcagcact ggatgaggtg atcagcgaga agctaaaccc tgcgcaccat
    32161 atcgacgccg ttgagagcaa attgtttggc attggcgcgg agagctacac ggttggctcc
    32221 aatgagtttt acctggggta tgccaccagc cgccagacgg cgctgtgcct ggacgccggg
    32281 cacttccacc cgactgaagt gatttccgac aagatttccg ccgccatgct gtatgtgccg
    32341 cagttgctgc tgcacgtcag tcgtccggtt cgctgggaca gcgatcacgt agtgctgctg
    32401 gatgatgaaa cccaggcaat tgccagtgag attgtgcgtc acgatctgtt tgaccgggtg
    32461 catatcggcc ttgacttctt cgatgcctct atcaaccgca ttgccgcgtg ggtcattggt
    32521 acacgcaata tgaaaaaagc cctgctgcgt gcgttgctgg aacctaccgc cgagctgcgc
    32581 aagttggaag cggcgggcga ttacactgcg cgtcaggcac tgctggaaga gcagaaatcg
    32641 ttgccgtggc aggcggtatg ggaaatgtat tgtcagcgtc acgatacccc agcgggtagc
    32701 gaatggctgg agagcgtgcg ggcttatgag aaagaaattt tgagtcagcg tgggtaaaca
    32761 ctgccagatg cggcacgagc gccttatccg gcctacagat cgtgcatctg acaacacccc
    32821 atttacagga acaccgaaca tgcaaaacat tactcagtcc tggtttgtcc agggaatgat
    32881 caaagccacc accgacgcct ggctgaaagg ctgggacgag cgcaacggcg gcaacctgac
    32941 gctacgcctg gatgacgctg acatcgcacc gtatcacgac aatttccacc aacaaccgcg
    33001 ttatatcccg ctcagccaac ccatgccttt actggcaaat acaccgttta tcgtcaccgg
    33061 ctcgggcaaa ttcttccgta acgtccagct tgatcctgcg gctaacttag gcgtcgtaaa
    33121 agtcgacagc gacggcgcgg gctaccatat tctctggggg ttaaccaacg aggccgttcc
    33181 aacttctgaa cttccggcac actttctctc ccattgcgaa cgcattaaag ccaccaacgg
    33241 taaagatcgg gtgatcatgc actgccatgc caccaacctg atcgccctca cctatgtact
    33301 ggaaaacgac acggcggtct tcactcgcca actgtgggaa ggcagcaccg agtgcctggt
    33361 ggtattcccg gatggcgttg gcattctgcc gtggatggtg cccggcacgg acgaaatcgg
    33421 tcaggcgacc gcacaagaaa tgcaaaaaca ttcgctggtg ttgtggccct tccacggtgt
    33481 gttcggttcc ggaccgacac tggatgaagc ctttggtttg atagataccg cagagaaatc
    33541 agcggaggtg ttagtcaaaa tctactcgat gggcggtatg aagcagacca ttacccgtga
    33601 ggagctgatt gcactcggtg agcgttttgg cgttacgcca ctggccagtg cgctggcact
    33661 ttaatatccc aacagatatc cggcggtgtg ggaaacgtcg ccggacagat ggatttgaac
    33721 ttaaggagag tcattatgag ttttatgttg gcattaccga aaattagcct gcatggtgct
    33781 ggggcaattg gcgatatggt aaatctggtt gcgcataaac agtggggaaa agcactgatc
    33841 gttaccgatg ggcaactggt taagttggga ttactggaca gcctgtttat cgcactggat
    33901 caatacgaaa tgtcttatca cttatttgat gaggtatttc ctaaccccac tgaagaactg
    33961 gtgcaaaaag gctacgctgc atttcaggat gccgcatgtg actacattat tgctttcggt
    34021 ggcggtagcc caattgatac tgcaaaagcc gtaaaaatcc ttaccgctaa tcctgcgcct
    34081 tctacggcgt actctggtgt tggtaaagtg acaaatcccg gcgtaccgct ggtggcaatc
    34141 aatacgactg ccggaacagc tgctgagatg accagcaatg cggtgatcat cgacaccgcg
    34201 cggcaggtaa aagaggtcat tatcgacccg aatattattc ccgatattgc ggtggatgat
    34261 gccagtgtaa tgctggagat cccggcctcg gttaccgccg ccacgggtat ggatgcactg
    34321 actcacgcag ttgaagctta tgtttctgtt ggtgcacatc cattaaccga cgctaacgca
    34381 ctggaagcta tccgtttaat tacgctgtgg ctgccaaaag cggtagatga tggtcataat
    34441 ctggaagctc gtgaacaaat ggcattcgcc cagtacctgg cgggtatggc atttaacagt
    34501 gctggtctgg gccttgttca tgcacttgcg catcagcccg gcgccaccca caacctgccg
    34561 catggtgtgt gcaatgctat cttgttgcca gttattgaaa actttaaccg cccgaatgcc
    34621 atcgcacgct ttgcccgcat tgctcaggca atgggtgtcg atacgcgtga tatgagtgaa
    34681 gaagcggcaa gtactgaagc aattaacgct atccgcgcat taagccatcg cgtgggtatt
    34741 ccggcaggat tcagccagct tggcatcacc aaagaagata ttgaaggctg gttagataaa
    34801 gccctggctg acccgtgtgt gccatgtaac ccgcgtaccg ccagccgcga cgaggtgcgc
    34861 gagctgtatc tggaggcatt atgatccgta aggcatttgt gatgcaggtt aaccccgacg
    34921 ctcacgaaga gtatcaacgt cggcataacc ccatctggcc ggaactggaa gcagtgttga
    34981 aatctcacgg tgcgcataac tacgccattt acctcgacaa agcgcgtaat ctgctgtttg
    35041 ccacggtaga gattgaatct gaagagcgct ggaatgcggt tgccagtact gatgtttgcc
    35101 aacgttggtg gaaatatatg accgacgtca tgcccgcgaa cccggataac agcccggtga
    35161 gtagcgaact gcaagaagtg ttttacctgc cgtaattgcc taatgtgccg gagacgcctc
    35221 cggtacaata tttttaattt cgatttgaaa aataaacata ttacaccttc agttaatata
    35281 tctcggtaac ttatatttcc ccgatgcatt gtgagtaaat cacaaaaata atgaataact
    35341 cattaatgat tcatgtggtt tatttaaata accattatgt gcattactcc gcaaatctgg
    35401 cctttcactc tgtctcaaat tgtcattgtt taccgacata tcacctctca tcacttaatc
    35461 gctccaccat tacaattgat taaaatatat tttatggagt ccgattaatg gcagctctta
    35521 ctgcaagctg tattgacctg aatattcagg gcaatggcgc ttattccatt ctgaagcagt
    35581 tggcgacaat ggcgttacaa aacggtttta tcaccgactc acaccagttc ctgcaaaccc
    35641 tgctgctgcg cgaaaaaatg cactctactg gatttggttc cggcatcgcc gtgccgcacg
    35701 gtaaaagcgc ctgcgttaaa caaccgttcg tgttattcgc ccgcaaagcg caggccattg
    35761 actggaaagc cagcgacggc gaagacgtca attgctggat ctgcctcggc gtgccgcaaa
    35821 gtggcgaaga ggatcaggtc aaaatcatcg gcacactgtg tcgcaaaatt atccatcagg
    35881 attttatcaa ccaactgcga cagggcgacg ccgaccaggt acttgctttg ttaaatcaaa
    35941 ccctcagctc ataaggaagt ggcgatggag tcatccttac gtattgtcgc gatcaccaac
    36001 tgccccgccg ggatcgctca cacctacatg gtggcggaag ccctggaaca gaaagcccgt
    36061 tctctcggcc ataccataaa agtggaaact caagggtcca gcggcgttga aaaccgctta
    36121 tccagcgaag agattgccgc tgccgattac gtcattctag ctaccgggcg tggcctgagc
    36181 ggtgatgacc gggcgcggtt tgccgggaag aaagtttatg agattgccat ctcccaggcg
    36241 ctgaaaaata tcgaccagat tttcagcgaa ttaccaacaa actcgcagct ttttgccgca
    36301 gatagcggcg tgaagctcgg caaacaggaa gtgcaaagcg gcagcgtgat gagccacctg
    36361 atggcgggcg tctctgcggc gctgccattt gtcatcggcg gcggtattct ggtggcgctt
    36421 gccaacatgc tggtgcagtt cggtttgcca tataccgata tgtcgaaagg tgcgccgtcg
    36481 tttacctggg tagttgaatc catcggttac ctcggcttca cctttatgat ccccatcatg
    36541 ggcgcttata ttgcctcgtc gattgccgat aaacccgctt tcgctccggc atttctggtt
    36601 tgctatctgg cgaacgataa agccctgctc ggcacccagt caggcgcggg cttcctcggc
    36661 gcggtggtgc tggggctggc gattggctat ttcgtcttct ggttccgtaa agtccgtctt
    36721 ggcaaagcat tacaaccgct gctcggctcc atgcttatcc cattcgttac tctgctggtt
    36781 tttggcgtgc tcacttacta cgttatcggg ccggtgatgt ccgacctcat gggcgggctg
    36841 ctccacttcc tgaataccat tcctccatca atgaagtttg cggcagcgtt tctggtcggt
    36901 gcgatgctgg cgttcgatat gggcggcccc atcaataaaa ccgcctggtt cttctgcttc
    36961 tcactgctgg aaaaacacat ttatgactgg tacgccatcg tcggcgttgt cgcgctgatg
    37021 ccgcccgtcg cggcgggtct tgcgacgttc attgcgccca aactgtttac ccgacaggaa
    37081 aaagaggctg ccagtagtgc cattgtggtc ggtgccacag tcgcaaccga accggctatt
    37141 ccttacgctc tggcagctcc gttgccgatg attaccgcca atacgctggc gggcggtatt
    37201 accggcgtgc tggtgatcgc tttcggaatc aaacgtctgg cacctggttt gggtatcttt
    37261 gacccgctga tcggcctgat gtcaccggta ggttcgtttt atctggtgct ggcgattggc
    37321 ctcgcgctga acatttcatt cattatcgtc ctgaaaggat tgtggttacg ccgcaaagcg
    37381 aaagccgcgc aacaggagct tgtccatgaa cattgagtta ctgcaacagt tgtgcgaagc
    37441 cagcgccgtt agcggcgatg aacaggaagt tcgcgacatt ctgataaaca cgctggaacc
    37501 ttgcgtgaat gaaatcacct ttgatgggct gggcagcttt gtcgcccgta agggaaataa
    37561 agggccgaaa gttgccgttg tcgggcatat ggatgaagtc ggctttatgg tcacccacat
    37621 cgacgagagc ggttttctgc gtttcaccac cattggtggc tggtggaatc agtcgatgct
    37681 caaccaccgg gtaaccatac gcacacacaa gggagtgaaa atccctggtg tgattggttc
    37741 cgtcgcgcct catgcgttaa cggaaaagca aaagcaacag ccgcagtcat ttgatgagat
    37801 gttcattgat attggcgcaa acagtcgcga tgaggtggaa aaacgcggtg tggcgatggg
    37861 cgattttatt agcccggaag ccaattttgc ctgctggggc gaagataaag tggtcggcaa
    37921 ggcgttggat aaccgcatcg gctgcgcaat gatggctgag ctactacaga cggtgaataa
    37981 tcccgaaatt acgctgtatg gcgttggcag tgtggaagaa gaagttgggc tacgcggggc
    38041 gcaaacctcg gctgaacaca ttaaaccgga tgtggtgatc gtgctggaca ccgctgtcgc
    38101 gggcgatgtt ccaggcattg ataacattaa atatccgctg aaactgggca acgggccggg
    38161 gttgatgctg tttgacaagc gctacttccc caaccagaaa ctggtagcag cgttaaaaag
    38221 ctgtgccacg cagaatgatt taccgctgca attttccacc atgaaaaccg gtgcgacgga
    38281 tggcgggcgc tacaacgtga tgggcggtgg gcgtccggtt gtcgcgctgt gtttgccaac
    38341 gcgttatctg cacgccaaca gcggcatgat ttcaaaagcc gattacgatg ctctgctcac
    38401 gctgatacgg ggttttctga ccaccttaac cgcggaaaaa gtcagcgcgt ttagccagtt
    38461 ccgtcaggtg gattaatgct aaacgaacgc cagttaaaga ttgtcgatct gctggagcaa
    38521 cagccgcgca cgcctggcga gctggcgcaa cagactggcg tttctggcag gaccatactg
    38581 cgtgatattg actatctcaa ctttaccctt aacggcaaag cccgcatttc tgacagtggt
    38641 agtgcgggct atcagctgga aatcttcgag cgccgcagct tttttcagtt gctgcaaaag
    38701 cacgataacg acgatcggct gctggcactg ttattactga atacctttac tccccgtgcg
    38761 caactcgcct cggcgcttaa tttgccggaa acgtgggtcg cagagcgtct gccccggtta
    38821 aaacagcgtt atgaacgcac ttgctgcctg gccagtcgcc ccggtttggg ccatttcatt
    38881 gatgagacag aagaaaaacg cgttatattg ctggcgaact tgctgcgcaa agatccgttt
    38941 ttaattccgc tggcgggcgt aacacgagac aaccttcagc atttatccac ggcctgcgac
    39001 aaccaacacc gctggccgct catgcagggt gattatctct ccagcctgat tctggcgatt
    39061 tacgccctgc gtaatcaact gaccgatgag tggccgcaat atcccggtga cgagataaaa
    39121 caaatcgttg aacatagcgg tctgtttctt ggtgataacg ctgtaagaac cctgacaggt
    39181 ttgatagaga aacagcatca gcaagcgcag ataatttctg ccgataatgt gcagcggttg
    39241 ctgcaaaggg tgccgggcat cgcgtcattg aatattattg atacgcagct ggttgagaac
    39301 attaccgggc atttattacg ttgccttgcc gcaccggtgt ggattgctga gcaccgccaa
    39361 agcagcatga ataacctgaa agccgcctgg cctgcggcct ttgatatgag tctgcacttt
    39421 attacgctac tgcgtgaaca gctcgatatt ccccttttcg acagcgatct gatcggtttg
    39481 tattttgcct gtgcgctgga acggcatcaa aacgaacgcc agccgatcat tttgctctcg
    39541 gaccagaacg cgattgccac tattaatcag ctcgccattg agcgcgatgt tttacattgt
    39601 cgggtgatta ttgcccgtag cttaagcgaa cttgttgcca ttcgcgaaga gattgagccg
    39661 ttattgatca ttaacaacag ccattattta ctggatgacg cggtaaataa ttacatcacc
    39721 gtaaaaaata tcattacggc tgccggtatc gaacaaataa aacattttct ggcgacggca
    39781 tttattcgcc aacagccgga gcgttttttc tctgctcccg gaagttttca ttattcgaat
    39841 gtacgcggtg aaagctggca acatattacc cggcaaattt gtgcgcaatt agtcgcacaa
    39901 caccatatta ccgccgatga agcacaacgc atcatcgccc gcgaaggcga aggtgaaaac
    39961 ctgattgtta atcgcctcgc catcccacat tgctggagcg aacaggagcg acgttttcgt
    40021 ggatttttta ttaccctcgc ccaaccagtt gaggtgaata acgaagtcat taaccatgtc
    40081 ttgatcgcct gcgccgccgc cgatgcgcgt catgagctaa aaatatttag ctatctggca
    40141 agcatattgt gtcagcatcc ggcggagatt attgccgggt taacaggata tgaggcattt
    40201 atggagttac ttcacaaggg gtgaactgtg tcggatgcgc cgcatccgac actgcattta
    40261 tcatcaaaaa cgtttaacgc aggctattat agtcatcaac catttcgttg tactcgcgta
    40321 atgctctcgg gaaagagtct tcgaccatcc agcttgagtt tgcgtcctga agttgctctt
    40381 tatcccagtc agagtacggg actttatctc tgatgcggcg aacgtatttt ttagcctcga
    40441 gttgatactg gcctgccgaa ttaataaacg agaaattcat gccgccttta tccgcttctt
    40501 tggcctgcgc cactagcgtt tccagttcag acactttctt cattgccgca tcaacatcaa
    40561 acttctcttg cgatatcagg ttattaattt gtttcgctga aatcatgact gccagagagt
    40621 agtagtggaa tgttttccct tctctttctt caatattttt cagttccgca agctggcgct
    40681 tatcgttaat ttcctgaatc gccgcatgat aagattctgc tacaggttca aaggcttcca
    40741 gattttttaa gaatgtctgg tgcagcgttt tccctttcgc aaacgcatcg tctttataat
    40801 tttcctgggt gtaatattta tccatttcgt taatggtatt acccaacgcc acggcagcat
    40861 cgatataact caccgccacg ccatcaattg gttgcagcgc aggcgttaac gccacagcac
    40921 tttttacgcc tttttcacac tcagcgaggt tggattcact aatgccgtag atgccataaa
    40981 cagtacgctc ttcaccggta ggcccctgtt taaaatcttt cagccagtca gcataacgcg
    41041 ccagactgct ctgtacaggg atctgtaact tgttatagca cttgatatag acatccattt
    41101 tttcgtcgaa aaccgcttgt tcatcgacca ccggttgcgc aggtgtttca ggtttagctt
    41161 ctgcttttgc aacgggagct tcaggagctg gtgcggcagg ttgactattg gcaggcgggg
    41221 gcgtttctgt ttcggctttt ttatcatcac aacccgtca
//
LOCUS       DAZKHF020000014        40543 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2201S31241
            SAMN43267800-rid25106413.denovo.014, whole genome shotgun sequence.
ACCESSION   DAZKHF020000014 DAZKHF020000000
VERSION     DAZKHF020000014.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267800
            Sequence Read Archive: SRR30307263
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 40543)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 40543)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 40543)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350581.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350581.2
            Genome Coverage       :: 151x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:21:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,652
            CDSs (total)                      :: 5,557
            Genes (coding)                    :: 5,320
            CDSs (with protein)               :: 5,320
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 237
            CDSs (without protein)            :: 237
            Pseudo Genes (ambiguous residues) :: 0 of 237
            Pseudo Genes (frameshifted)       :: 102 of 237
            Pseudo Genes (incomplete)         :: 130 of 237
            Pseudo Genes (internal stop)      :: 52 of 237
            Pseudo Genes (multiple problems)  :: 41 of 237
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..40543
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267800-rid25106413.denovo.014"
                     /serotype="O 26:H11"
                     /isolate="2201S31241"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-01"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            complement(164..240)
                     /locus_tag="ACHOSC_RS03005"
                     /old_locus_tag="pgaptmp_000601"
     tRNA            complement(164..240)
                     /locus_tag="ACHOSC_RS03005"
                     /old_locus_tag="pgaptmp_000601"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(204..206),aa:Arg,seq:acg)
     gene            complement(304..380)
                     /locus_tag="ACHOSC_RS03010"
                     /old_locus_tag="pgaptmp_000602"
     tRNA            complement(304..380)
                     /locus_tag="ACHOSC_RS03010"
                     /old_locus_tag="pgaptmp_000602"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(344..346),aa:Arg,seq:acg)
     gene            complement(384..476)
                     /locus_tag="ACHOSC_RS03015"
                     /old_locus_tag="pgaptmp_000603"
     tRNA            complement(384..476)
                     /locus_tag="ACHOSC_RS03015"
                     /old_locus_tag="pgaptmp_000603"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(440..442),aa:Ser,seq:gct)
     gene            complement(792..977)
                     /gene="csrA"
                     /locus_tag="ACHOSC_RS03020"
                     /old_locus_tag="pgaptmp_000604"
     CDS             complement(792..977)
                     /gene="csrA"
                     /locus_tag="ACHOSC_RS03020"
                     /old_locus_tag="pgaptmp_000604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001774971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon storage regulator CsrA"
                     /protein_id="HGU4546601.1"
                     /translation="MLILTRRVGETLMIGDEVTVTVLGVKGNQVRIGVNAPKEVSVHR
                     EEIYQRIQAEKSQQSSY"
     gene            complement(1212..3842)
                     /gene="alaS"
                     /locus_tag="ACHOSC_RS03025"
                     /old_locus_tag="pgaptmp_000605"
     CDS             complement(1212..3842)
                     /gene="alaS"
                     /locus_tag="ACHOSC_RS03025"
                     /old_locus_tag="pgaptmp_000605"
                     /EC_number="6.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004813 - alanine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006419 - alanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--tRNA ligase"
                     /protein_id="HGU4546602.1"
                     /translation="MSKSTAEIRQAFLDFFHSKGHQVVASSSLVPHNDPTLLFTNAGM
                     NQFKDVFLGLDKRNYSRATTSQRCVRAGGKHNDLENVGYTARHHTFFEMLGNFSFGDY
                     FKHDAIQFAWELLTSEKWFALPKERLWVTVYESDDEAYEIWEKEVGIPRERIIRIGDN
                     KGAPYASDNFWQMGDTGPCGPCTEIFYDHGDHIWGGPPGSPEEDGDRYIEIWNIVFMQ
                     FNRQADGTMEPLPKPSVDTGMGLERIAAVLQHVNSNYDIDLFRTLIQAVAKVTGATDL
                     SNKSLRVIADHIRSCAFLIADGVMPSNENRGYVLRRIIRRAVRHGNMLGAKETFFYKL
                     VGPLIDVMGSAGEDLKRQQAQVEQVLKTEEEQFARTLERGLALLDEELAKLSGDTLDG
                     ETAFRLYDTYGFPVDLTADVCRERNIKVDEAGFEAAMEEQRRRAREASGFGADYNAMI
                     RVDSASEFKGYDHLELNGKVTALFVDGKAVDAINAGQEAVVVLDQTPFYAESGGQVGD
                     KGELKGANFSFAVEDTQKYGQAIGHIGKLAAGSLKVGDAVQADVDEARRARIRLNHSA
                     THLMHAALRQVLGTHVSQKGSLVNDKVLRFDFSHNEAMKPEEIRAVEDLVNAQIRRNL
                     PIETNIMDLEAAKAKGAMALFGEKYDERVRVLSMGDFSTELCGGTHASRTGDIGLFRI
                     ISESGTAAGVRRIEAVTGEGAITTVHADSDRLSEVAHLLKGDSNNLADKVRSVLERTR
                     QLEKELQQLKEQAAAQESANLSSKAIDVNGVKLLVSELSGVEPKMLRTMVDDLKNQLG
                     STIIVLATVAEGKVSLIAGVSKDVTDRVKAGELIGMVAQQVGGKGGGRPDMAQAGGTD
                     AAALPAALASVKGWVSAKLQ"
     gene            complement(3970..4470)
                     /gene="recX"
                     /locus_tag="ACHOSC_RS03030"
                     /old_locus_tag="pgaptmp_000606"
     CDS             complement(3970..4470)
                     /gene="recX"
                     /locus_tag="ACHOSC_RS03030"
                     /old_locus_tag="pgaptmp_000606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination regulator RecX"
                     /protein_id="HGU4546603.1"
                     /translation="MTESTSRRPAYARLLDRAVRILAVRDHSEQELRRKLVAPIMGKN
                     GPEEIDATAEDYERVIAWCHEHGYLDDSRFVARFIASRSRKGYGPARIRQELNQKGIS
                     REATEKAMRECDIDWCALARDQATRKYGEPLPTVFSEKVKIQRFLLYRGYLMEDIQDI
                     WRNFAD"
     gene            complement(4713..5774)
                     /gene="recA"
                     /locus_tag="ACHOSC_RS03035"
                     /old_locus_tag="pgaptmp_000607"
     CDS             complement(4713..5774)
                     /gene="recA"
                     /locus_tag="ACHOSC_RS03035"
                     /old_locus_tag="pgaptmp_000607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008094 - ATP-dependent activity, acting
                     on DNA [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase RecA"
                     /protein_id="HGU4546604.1"
                     /translation="MAIDENKQKALAAALGQIEKQFGKGSIMRLGEDRSMDVETISTG
                     SLSLDIALGAGGLPMGRIVEIYGPESSGKTTLTLQVIAAAQREGKTCAFIDAEHALDP
                     IYARKLGVDIDNLLCSQPDTGEQALEICDALARSGAVDVIVVDSVAALTPKAEIEGEI
                     GDSHMGLAARMMSQAMRKLAGNLKQSNTLLIFINQIRMKIGVMFGNPETTTGGNALKF
                     YASVRLDIRRIGAVKEGENVVGSETRVKVVKNKIAAPFKQAEFQILYGEGINFYGELV
                     DLGVKEKLIEKAGAWYSYKGEKIGQGKANATAWLKDNPETAKEIEKKVRELLLSNPNS
                     TPDFSVDDSEGVAETNEDF"
     gene            complement(5854..6351)
                     /gene="pncC"
                     /locus_tag="ACHOSC_RS03040"
                     /old_locus_tag="pgaptmp_000608"
     CDS             complement(5854..6351)
                     /gene="pncC"
                     /locus_tag="ACHOSC_RS03040"
                     /old_locus_tag="pgaptmp_000608"
                     /EC_number="3.5.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinamide-nucleotide amidase"
                     /protein_id="HGU4546605.1"
                     /translation="MTDSELMQLSEQVGQALKARGATVTTAESCTGGWVAKVITDIAG
                     SSAWFERGFVTYSNEAKAQMIGVREETLAQHGAVSEPVVVEMAIGALKAARADYAVSI
                     SGIAGPDGGSEEKPVGTVWFAFATARGEGITRRECFSGDRDAVRRQATAYALQTLWQQ
                     FLQNT"
     gene            complement(6496..7581)
                     /gene="mltB"
                     /locus_tag="ACHOSC_RS03045"
                     /old_locus_tag="pgaptmp_000609"
     CDS             complement(6496..7581)
                     /gene="mltB"
                     /locus_tag="ACHOSC_RS03045"
                     /old_locus_tag="pgaptmp_000609"
                     /EC_number="4.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lytic murein transglycosylase B"
                     /protein_id="HGU4546606.1"
                     /translation="MFKRRYVTLLPLFVLLAACSSKPKPTETETTTGTPSGGFLLEPQ
                     HNVMQMGGDFANNPNAQQFIDKMVNKHGFDRQQLQEILSQAKRLDSVLRLMDNQAPTT
                     SVKPPSGPNGAWLRYRKKFITPDNVQNGVVFWNQYEDALNRAWQVYGVPPEIIVGIIG
                     VETRWGRVMGKTRILDALATLSFNYPRRAEYFSGELETFLLMARDEQDDPLNLKGSFA
                     GAMGYVQFMPSSYKQYAVDFSGDGHINLWDPVDAIGSVANYFKAHGWVKGDQVAVMAN
                     GQAPGLPNGFKTRYSISQLATAGLTPQQPLGNHQQASLLRLDVGTGYQYWYGLPNFYT
                     ITRYNHSTHYAMAVWQLGQAVALARVQ"
     gene            7837..8400
                     /gene="srlA"
                     /locus_tag="ACHOSC_RS03050"
                     /old_locus_tag="pgaptmp_000610"
                     /gene_synonym="gutA"
     CDS             7837..8400
                     /gene="srlA"
                     /locus_tag="ACHOSC_RS03050"
                     /old_locus_tag="pgaptmp_000610"
                     /gene_synonym="gutA"
                     /EC_number="2.7.1.198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIC"
                     /protein_id="HGU4546607.1"
                     /translation="MIETITHGAEWFIGLFQKGGEVFTGMVTGILPLLISLLVIMNAL
                     INFIGQHRIERFAQRCAGNPVSRYLLLPCIGTFVFCNPMTLSLGRFMPEKYKPSYYAA
                     ASYSCHSMNGLFPHINPGELFVYLGIASGLTTLNLPLGPLAVSYLLVGLVTNFFRGWV
                     TDLTTAIFEKKMGIQLEQKVHLAGATS"
     gene            8397..9356
                     /gene="srlE"
                     /locus_tag="ACHOSC_RS03055"
                     /old_locus_tag="pgaptmp_000611"
                     /gene_synonym="gutE"
     CDS             8397..9356
                     /gene="srlE"
                     /locus_tag="ACHOSC_RS03055"
                     /old_locus_tag="pgaptmp_000611"
                     /gene_synonym="gutE"
                     /EC_number="2.7.1.198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIB"
                     /protein_id="HGU4546608.1"
                     /translation="MTHIRIEKGTGGWGGPLELKATPGKKIVYITAGTRPAIVDKLAQ
                     LTGWQAIDGFREGEPAEAEIGVAVIDCGGTLRCGIYPKRRIPTINIHSTGKSGPLAQY
                     IVEDIYVSGVKEENITVVGDATPQPSSVGRDYDTSKKITEQSDGLLAKVGMGMGSAVA
                     VLFQSGRDTIDTVLKTILPFMAFVSALIGIIMASGLGDWIAHGLAPLASHPLGLVMLA
                     LICSFPLLSPFLGPGAVIAQVIGVLIGVQIGLGNIPPHLALPALFAINAQAACDFIPV
                     GLSLAEARQDTVRVGVPSVLVSRFLTGAPTVLIAWFVSGFIYQ"
     gene            9367..9738
                     /gene="srlB"
                     /locus_tag="ACHOSC_RS03060"
                     /old_locus_tag="pgaptmp_000612"
                     /gene_synonym="gutB"
     CDS             9367..9738
                     /gene="srlB"
                     /locus_tag="ACHOSC_RS03060"
                     /old_locus_tag="pgaptmp_000612"
                     /gene_synonym="gutB"
                     /EC_number="2.7.1.198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIA"
                     /protein_id="HGU4546609.1"
                     /translation="MSVIYQTTITRIGASATDALSDQMLITFREGAPADLEEYCFIHC
                     HGELKGALHPGLQFTLGQHRYPVTAVGSVAEDNLRELGHVTLRFDGLNEAEFPGTVHV
                     AGPVPDDIAPGSVLKFESVKE"
     gene            9742..10521
                     /gene="srlD"
                     /locus_tag="ACHOSC_RS03065"
                     /old_locus_tag="pgaptmp_000613"
     CDS             9742..10521
                     /gene="srlD"
                     /locus_tag="ACHOSC_RS03065"
                     /old_locus_tag="pgaptmp_000613"
                     /EC_number="1.1.1.140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sorbitol-6-phosphate dehydrogenase"
                     /protein_id="HGU4546610.1"
                     /translation="MNQVAVVIGGGQTLGAFLCHGLAAEGYRVAVVDIQSDKAANVAQ
                     EINAEYGESMAYGFGADATSEQSVLALSRGVDEIFGRVDLLVYSAGIAKAAFISDFQL
                     GDFDRSLQVNLVGYFLCAREFSRLMIRDGIQGRIIQINSKSGKVGSKHNSGYSAAKFG
                     GVGLTQSLALDLAEYGITVHSLMLGNLLKSPMFQSLLPQYATKLGIKPDQVEQYYIDK
                     VPLKRGCDYQDVLNMLLFYASPKASYCTGQSINVTGGQVMF"
     gene            10626..10985
                     /gene="gutM"
                     /locus_tag="ACHOSC_RS03070"
                     /old_locus_tag="pgaptmp_000614"
     CDS             10626..10985
                     /gene="gutM"
                     /locus_tag="ACHOSC_RS03070"
                     /old_locus_tag="pgaptmp_000614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator GutM"
                     /protein_id="HGU4546611.1"
                     /translation="MVSALITVAVIAWCAQLALGGWQISRFNRAFDTLCQQGRVGVGR
                     SSGRFKPRVVVAIALDDQQRIVDTLFMKGLTVFARPQKIPAITGMHAGDLQPDVIFPH
                     DPLSQNALSLALKLKRG"
     gene            11052..11825
                     /gene="srlR"
                     /locus_tag="ACHOSC_RS03075"
                     /old_locus_tag="pgaptmp_000615"
                     /gene_synonym="gutR"
     CDS             11052..11825
                     /gene="srlR"
                     /locus_tag="ACHOSC_RS03075"
                     /old_locus_tag="pgaptmp_000615"
                     /gene_synonym="gutR"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucitol operon DNA-binding transcriptional
                     repressor SrlR"
                     /protein_id="HGU4546612.1"
                     /translation="MKPRQRQAAILEYLQKQGKCSVEELAQYFDTTGTTIRKDLVILE
                     HAGTVIRTYGGVVLNKEESDPPIDHKTLINTHKKELIAEAAVSFIHDGDSIILDAGST
                     VLQMVPLLSRFNNITVMTNSLHIVNALSELDNEQTILMPGGTFRKKSASFHGQLAENA
                     FEHFSFDKLFMGTDGIDLNAGVTTFNEVYTVSKAMCNAAREVILMADSSKFGRKSPNV
                     VCSLESVDKLITDAGIDPAFRQALEEKGIDVIITGESNE"
     gene            11818..12783
                     /gene="gutQ"
                     /locus_tag="ACHOSC_RS03080"
                     /old_locus_tag="pgaptmp_000616"
     CDS             11818..12783
                     /gene="gutQ"
                     /locus_tag="ACHOSC_RS03080"
                     /old_locus_tag="pgaptmp_000616"
                     /EC_number="5.3.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311591.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arabinose-5-phosphate isomerase GutQ"
                     /protein_id="HGU4546613.1"
                     /translation="MSEALLNAGRQTLMLELQEASRLPERLGDDFVRAANIILHCEGK
                     VVVSGIGKSGHIGKKIAATLASTGTPAFFVHPAEALHGDLGMIESRDVMLFISYSGGA
                     KELDLIIPRLEDKSIALLAMTGKPTSPLGLAAKAVLDISVEREACPMHLAPTSSTVNT
                     LMMGDALAMAVMQARGFNEEDFARSHPAGALGARLLNKVHHLMRRDDAIPQVALTASV
                     MDAMLELSRTGLGLVAVCDAQQQVQGVFTDGDLRRWLVGGGALTTPVNEAMTTGGTTL
                     QAQSRAIDAKEILMKRKITAAPVVDENGKLTGAINLQDFYQAGII"
     gene            complement(12780..14294)
                     /gene="norR"
                     /locus_tag="ACHOSC_RS03085"
                     /old_locus_tag="pgaptmp_000617"
     CDS             complement(12780..14294)
                     /gene="norR"
                     /locus_tag="ACHOSC_RS03085"
                     /old_locus_tag="pgaptmp_000617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311592.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitric oxide reductase transcriptional regulator
                     NorR"
                     /protein_id="HGU4546614.1"
                     /translation="MSFSVDVLANIAIELQRGIGHQDRFQRLITTLRQVLECDASALL
                     RYDSRQFIPLAIDGLAKDVLGRRFALEGHPRLEAIARAGDVVRFPADSELPDPYDGLI
                     PGQESLKVHACVGLPLFAGQNLIGALTLDGMQPDQFDVFSDEELRLIAALAAGALSNA
                     LLIEQLESQNMLPGDAAPFEAVKQTQMIGLSPGMTQLKKEIEIVAASDLNVLISGETG
                     TGKELVAKAIHEASPRAVNPLVYLNCAALPESVAESELFGHVKGAFTGAISNRSGKFE
                     MADNGTLFLDEIGELSLALQAKLLRVLQYGDIQRVGDDRSLRVDVRVLAATNRDLREE
                     VLAGRFRADLFHRLSVFPLSVPPLRERGDDVILLAGYFCEQCRLRLGLSRVVLSAGAR
                     NLLQHYRFPGNVRELEHAIHRAVVLARATRSGDEVILEAQHFAFPEVTLPPPEAAAVP
                     VVKQNLREATEAFQRETIRQALAQNHHNWAACARMLETDVANLHRLAKRLGLKD"
     gene            14481..15920
                     /gene="norV"
                     /locus_tag="ACHOSC_RS03090"
                     /old_locus_tag="pgaptmp_000618"
     CDS             14481..15920
                     /gene="norV"
                     /locus_tag="ACHOSC_RS03090"
                     /old_locus_tag="pgaptmp_000618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016966 - nitric oxide reductase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic nitric oxide reductase
                     flavorubredoxin"
                     /protein_id="HGU4546615.1"
                     /translation="MSIVVKNNIHWVGQRDWEVRDFHGTEYKTLRGSSYNSYLIREEK
                     NVLIDTVDHKFSREFVQNLRNEIDLADIDYIVINHAEEDHAGALTELMAQIPDTPIYC
                     TANAIDSINGHHHHPEWNFNVVKTGDTLDIGNGKQLIFVETPMLHWPDSMMTYLTGDA
                     VLFSNDAFGQHYCDEHLFNDEVDQTELFEQCQRYYANILTPFSRLVTPKITEILGFNL
                     PVDMIATSHGVVWRDNPTQIVELYLKWAADYQEDRITIFYDTMSNNTRMMADAIAQGI
                     AETDPRVAVKIFNVARSDKNEILTNVFRSKGVLVGTSTMNNVMMPKIAGLVEEMTGLR
                     FRNKRASAFGSHGWSGGAVDRLSTRLQDAGFEMSLSLKAKWRPDQDALELCREHGREI
                     ARQWALAPLPQSTVNTVVKEETSATTTADLGPRMQCSVCQWIYDPAKGEPMQDVAPGT
                     PWSEVPDNFLCPECSLGKDVFEELASEAK"
     gene            15917..17050
                     /gene="norW"
                     /locus_tag="ACHOSC_RS03095"
                     /old_locus_tag="pgaptmp_000619"
     CDS             15917..17050
                     /gene="norW"
                     /locus_tag="ACHOSC_RS03095"
                     /old_locus_tag="pgaptmp_000619"
                     /EC_number="1.18.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016731 - oxidoreductase activity, acting
                     on iron-sulfur proteins as donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH:flavorubredoxin reductase NorW"
                     /protein_id="HGU4546616.1"
                     /translation="MSNGIVIIGSGFAARQLVKNIRKQDATIPLTLIAADSMDEYNKP
                     DLSHVISQGQRADDLTRQTAGEFAEQFNLRLFPHTWVTDIDAEARVVKSQNNQWQYDK
                     LVLATGASAFVPPVPGRELMLTLNSQQEYRACETQLRDARRVLIVGGGLIGSELAMDF
                     CRAGKAVTLIDNAASILASLMPPEVSSRLQHRLTEMGVHLLLKSQLQGLEKTDSGILA
                     TLDRQRSIEVDAVIAATGLRPETALARRAGLTINRGVCVDSYLQTSNADIYALGDCAE
                     INGQVLPFLQPIQLSAMVLAKNLLGNNTPLKLPAMLVKIKTPELPLHLAGETQRQDLR
                     WQINTERQGMVARGVDDADQLRAFVVSEDRMKEAFGLLKTLPM"
     gene            complement(17276..19528)
                     /gene="hypF"
                     /locus_tag="ACHOSC_RS03100"
                     /old_locus_tag="pgaptmp_000620"
     CDS             complement(17276..19528)
                     /gene="hypF"
                     /locus_tag="ACHOSC_RS03100"
                     /old_locus_tag="pgaptmp_000620"
                     /EC_number="6.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003998 - acylphosphatase activity
                     [Evidence IEA];
                     GO_function: GO:0016743 - carboxyl- or
                     carbamoyltransferase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyltransferase HypF"
                     /protein_id="HGU4546617.1"
                     /translation="MAKNTSCGVQLRIRGKVQGVGFRPFVWQLAQQLNLHGDVCNDGD
                     GVEVRLLEDPETFLVQLHQHCPPLARIDSVEREPFIWSQLPTEFTIRQSAGGAMNTQI
                     VPDAATCPACLAEMNTPGERRYRYPFINCTHCGPRFTIIRAMPYDRPFTVMAEFPLCP
                     ACDKEYRDPLDRRFHAQPVACPECGPHLEWVSHGEHAEQEAALQAAIAQLKMGKIVAI
                     KGIGGFHLACDARNSNAVATLRERKHRPAKPLAVMLPVADGLPDAARQLLTTPAAPIV
                     LVDKKYVPELCDDIAPDLNEVGVMLPANPLQHLLLQELQCPLVMTSGNLSGKPPAISN
                     EQALADLQGIADGFLIHNRDIVQRMDDSVVRESGEMLRRSRGYVPDALALPPGFKNVP
                     PVLCLGADLKNTFCLVRGEQAVLSQHLGDLSDDGIQMQWREALRLMQNIYDFTPQYVV
                     HDVHPGYVSSQWASEMNMPTQTVLHHHAHAAACLAEHQWPLDGGDVIALTLDGIGMGE
                     NGALWGGECLRVNYRECEHLGGLPAVALPGGDLAAKQPWRNLLAQCLRFVPEWQNYSE
                     TASVQQQNWSVLARAIERGINAPLASSCGRLFDAVAAALGCAPATLSYEGEAACALEA
                     LAASCHGVTHPVTMPLVDNQLDLATFWQQWLNWQAPVNQRAWAFHDALAQGFAALMRE
                     QATMRGITTLVFSGGVIHNRLLRARLAHYLADFTLLFPQSLPAGDGGLSLGQGVIAAA
                     RWLAGEVQNG"
     gene            complement(19681..20208)
                     /gene="hydN"
                     /locus_tag="ACHOSC_RS03105"
                     /old_locus_tag="pgaptmp_000621"
     CDS             complement(19681..20208)
                     /gene="hydN"
                     /locus_tag="ACHOSC_RS03105"
                     /old_locus_tag="pgaptmp_000621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport protein HydN"
                     /protein_id="HGU4546618.1"
                     /translation="MNRFIIADASKCIGCRTCEVACVVSHQENQDCASLTPETFLPRI
                     HVIKGVNISTATVCRQCEDAPCANVCPNGAISRDKGFVHVMQERCIGCKTCVVACPYG
                     AMEVVVRPVIRNSGVGLNVRADKAEANKCDLCNHREDGPACMAACPTHALICVDRNKL
                     EQLSAEKRRRTALMF"
     gene            complement(20357..21367)
                     /gene="ascG"
                     /locus_tag="ACHOSC_RS03110"
                     /old_locus_tag="pgaptmp_000622"
     CDS             complement(20357..21367)
                     /gene="ascG"
                     /locus_tag="ACHOSC_RS03110"
                     /old_locus_tag="pgaptmp_000622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417194.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator AscG"
                     /protein_id="HGU4546619.1"
                     /translation="MTTMLEVAKRAGVSKATVSRVLSGNGYVSQETKDRVFQAVEESG
                     YRPNLLARNLSAKSTQTLGLVVTNTLYHGIYFSELLFHAARMAEEKGRQLLLADGKHS
                     AEEERQAIQYLLDLRCDAIMIYPRFLSVDEIDDIIDAHSQPIMVLNRRLRKNSSHSVW
                     CDHKQTSFNAVAELINAGHQEIAFLTGSMDSPTSVERLAGYKDALAQHGIALNEKLIA
                     NGRWTPASGAEGVEMLLERGAKFSALVASNDDMAIGAMKALHERGVAVPEQVSVIGFD
                     DIAIAPYIVPALSSVKIPVTEMIQEIIGRLIFMLDGGDFSPPKTFSGKLICRDSLIAL
                     SR"
     gene            21627..23084
                     /gene="ascF"
                     /locus_tag="ACHOSC_RS03115"
                     /old_locus_tag="pgaptmp_000623"
     CDS             21627..23084
                     /gene="ascF"
                     /locus_tag="ACHOSC_RS03115"
                     /old_locus_tag="pgaptmp_000623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS cellobiose/arbutin/salicin transporter
                     subunit IIBC"
                     /protein_id="HGU4546620.1"
                     /translation="MAKNYAALARSVIAALGGVDNISAVTHCMTRLRFVIKDDQLIDS
                     PTLKTIPGVLGVVRSDNQCQVIIGNTVSQAFQEVVSLLPGDMQPALPVGKPKLTLRRI
                     GAGILDALIGTMSPLIPAIIGGSMVKLLAMILEMSGVLTKGSPTLTILNVIGDGAFFF
                     LPLMVAASAAIKFKTNMSLAIAIAGVLVHPSFIELMAKAAQGEHVEFALIPVTAVKYT
                     YTVIPALVMTWCLSYIERWVDRITPAVTKNFLKPMLIVLIAAPLAILLIGPIGIWIGS
                     AISALVYTIHGYLGWLSVAIMGALWPLLVMTGMHRVFTPTIIQTIAETGKEGMVMPSE
                     IGANLSLGGSSLAVAWKTKNPELRQTALAAAASAIMAGISEPALYGVAIRLKRPLIAS
                     LISGFICGAVAGMAGLASHSMAAPGLFTSVQFFDPANPMSIVWVFAVMALAVMLSFIL
                     TLLLGFEDIPVEEATAEARKHQSVQPTVAKEVSLN"
     gene            23093..24517
                     /gene="ascB"
                     /locus_tag="ACHOSC_RS03120"
                     /old_locus_tag="pgaptmp_000624"
     CDS             23093..24517
                     /gene="ascB"
                     /locus_tag="ACHOSC_RS03120"
                     /old_locus_tag="pgaptmp_000624"
                     /EC_number="3.2.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase AscB"
                     /protein_id="HGU4546621.1"
                     /translation="MSVFPEGFLWGGALAANQSEGAFREGGKGLTTVDMIPHGEHRMA
                     VKLGLEKRFQLRDDEFYPSHEATDFYHRYKEDIALMAEMGFKVFRTSIAWSRLFPQGD
                     EITPNQQGIAFYRSVFEECKKYGIEPLVTLCHFDVPMHLVTEYGSWRNRKLVEFFSRY
                     ARTCFEAFDGLVKYWLTFNEINIMLHSPFSGAGLVFEEGENQDQVKYQAAHHQLVASA
                     LATKIAHEVNPQNQVGCMLAGGNFYPYSCKPEDVWAALEKDRENLFFIDVQARGAYPA
                     YSARVFREKGVTIDKAPGDDEILKNTVDFVSFSYYASRCASAEMNANNSSAANVVKSL
                     RNPYLQVSDWGWGIDPLGLRITMNMMYDRYQKPLFLVENGLGAKDELAANGEINDDYR
                     ISYLREHIRAMGEAIADGIPLMGYTTWGCIDLVSASTGEMSKRYGFVYVDRDDAGNGT
                     LTRTRKKSFWWYKKVIASNGEDLE"
     gene            complement(24631..25050)
                     /locus_tag="ACHOSC_RS03125"
                     /old_locus_tag="pgaptmp_000625"
     CDS             complement(24631..25050)
                     /locus_tag="ACHOSC_RS03125"
                     /old_locus_tag="pgaptmp_000625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001696729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HGU4546622.1"
                     /translation="MTANAARAVKATRELVNAVPFLGGSDSEDDYREALELVEYLIEE
                     DDTNPLIDFLASRIAEYENNNEKFAEFDKAVAAMPVGVALLRTLIDQHNLTYADLKNE
                     IGSKSLVSQILSGQRSLTISHIKALSARFGVKPEWFL"
     gene            complement(25047..25358)
                     /locus_tag="ACHOSC_RS03130"
                     /old_locus_tag="pgaptmp_000626"
     CDS             complement(25047..25358)
                     /locus_tag="ACHOSC_RS03130"
                     /old_locus_tag="pgaptmp_000626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016233494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HigB family
                     toxin"
                     /protein_id="HGU4546623.1"
                     /translation="MHIISKAPFEESARKYPNDALAHQALYRVIKETDFSTPEEMRTA
                     FPNLDNFRYRNKWYVLDVGGNNLRVIAYINFVNKRFFVKHITNHAEYDKLTRYYRENK
                     E"
     gene            complement(25532..26275)
                     /locus_tag="ACHOSC_RS03135"
                     /old_locus_tag="pgaptmp_000627"
     CDS             complement(25532..26275)
                     /locus_tag="ACHOSC_RS03135"
                     /old_locus_tag="pgaptmp_000627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001399699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4546624.1"
                     /translation="MCAFLLSLVLPAQATSFTEYLPMSDSEYAQKRALKPLLTMPYDA
                     EQTWHFRKVGVAGVTLEKMPNEEDYWQLTAKDRAGKSWTVPVGMLANIAGKGQLYRAD
                     LDRNGIQDLVIWLPSTGNGLAPYAHLILMTFTREGRPCVFEPRGFYTASKTGVDDLLD
                     LQGNGHTQLLDMQFNSGYWITSLYQVKDAKWQRVHGWFGKLSYPALTRFTYTPNRKLV
                     LKPIAGRDPQTEDLAQTQRCLIKGDVLEG"
     gene            complement(26318..26788)
                     /gene="hycI"
                     /locus_tag="ACHOSC_RS03140"
                     /old_locus_tag="pgaptmp_000628"
     CDS             complement(26318..26788)
                     /gene="hycI"
                     /locus_tag="ACHOSC_RS03140"
                     /old_locus_tag="pgaptmp_000628"
                     /EC_number="3.4.23.51"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004190 - aspartic-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase maturation peptidase HycI"
                     /protein_id="HGU4546625.1"
                     /translation="MTDVLLCVGNSMMGDDGAGPLLAEKCAAAPKGNWVVIDGGSAPE
                     NDIVAIRELRPTRLLIVDATDMGLNPGEIRIIDPDDIAEMFMMTTHNMPLNYLIDQLK
                     EDIGEVIFLGIQPDIVGFYYPMTQPIKDAVETVYQRLEGWEGNGGFAQLAVEEE"
     gene            complement(26781..27191)
                     /gene="hycH"
                     /locus_tag="ACHOSC_RS03145"
                     /old_locus_tag="pgaptmp_000629"
     CDS             complement(26781..27191)
                     /gene="hycH"
                     /locus_tag="ACHOSC_RS03145"
                     /old_locus_tag="pgaptmp_000629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase assembly protein HycH"
                     /protein_id="HGU4546626.1"
                     /translation="MSEKVVFSQLSRKFIDENDATPAEAQQVVYYSLAIGHHLGVIDC
                     LEAALTCPWDEYLAWIATLEAGSEARRKMEGVPKYGEIVIDINHVPMLANAFDKARAA
                     QTSQQKEWSTMLLSMLHDIHQENAIYLMVRRLRD"
     gene            complement(27188..27955)
                     /gene="hycG"
                     /locus_tag="ACHOSC_RS03150"
                     /old_locus_tag="pgaptmp_000630"
     CDS             complement(27188..27955)
                     /gene="hycG"
                     /locus_tag="ACHOSC_RS03150"
                     /old_locus_tag="pgaptmp_000630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase subunit HycG"
                     /protein_id="HGU4546627.1"
                     /translation="MSNLLGPRDANGIPVPMTVDESIASMKASLLKKIKRSAYVYRVD
                     CGGCNGCEIEIFATLSPLFDAERFGIKVVPSPRHADILLFTGAVTRAMRSPALRAWQS
                     APDPKICISYGACGNSGGIFHDLYCVWGGTDKIVPVDVYIPGCPPTPAATLYGFAMAL
                     GLLEQKIHARGPGEQDEQPAEILHGDMVQPLRVKVDREARRLAGYRYGRQIADDYLTQ
                     LGQGEEQVARWLEAENDPRLNEIVSHLNHVVEEARIR"
     gene            complement(27955..28497)
                     /gene="hycF"
                     /locus_tag="ACHOSC_RS03155"
                     /old_locus_tag="pgaptmp_000631"
     CDS             complement(27955..28497)
                     /gene="hycF"
                     /locus_tag="ACHOSC_RS03155"
                     /old_locus_tag="pgaptmp_000631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase subunit HycF"
                     /protein_id="HGU4546628.1"
                     /translation="MFTFIKKVIKTGTATSSYPLEPIAVDKNFRGKPEQNPQQCIGCA
                     ACVNACPSNALTVETDLATGELAWEFNLGRCIFCGRCEEVCPTAAIKLSQEYELAVWK
                     KEDFLQQSRFVLCSCRVCNRPFAVQKEIDYAIALLKHNGDSRAENHRESFETCPECKR
                     QKCLVPSDRIELTRHMKEAI"
     gene            complement(28507..30216)
                     /gene="hycE"
                     /locus_tag="ACHOSC_RS03160"
                     /old_locus_tag="pgaptmp_000632"
     CDS             complement(28507..30216)
                     /gene="hycE"
                     /locus_tag="ACHOSC_RS03160"
                     /old_locus_tag="pgaptmp_000632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005130590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase subunit HycE"
                     /protein_id="HGU4546629.1"
                     /translation="MSEEKLGQHYLAALNEAFPGVVLDHAWQTKDQLTVTVKVNYLPE
                     VVEFLYYKQGGWLSVLFGNDERKLNGHYAVYYVLSMEKGTKCWVTVRVEVDANKPEYP
                     SVTPRVPAAVWGEREVRDMYGLIPVGLPDERRLVLPDDWPDELYPLRKDSMDYRQRPA
                     PTTDAETYEFINELGDKKNNVVPIGPLHVTSDEPGHFRLFVDGENIIDADYRLFYVHR
                     GMEKLAETRMGYNEVTFLSDRVCGICGFAHSTAYTTSVENAMGIQVPERAQMIRAILL
                     EVERLHSHLLNLGLACHFTGFDSGFMQFFRVRETSMKMAEILTGARKTYGLNLIGGIR
                     RDLLKDDMIQTRQLAQQMRREVQELVDVLLSTPNMEQRTVGIGRLDPEIARDFSNVGP
                     MVRASGHARDTRADHPFVGYGLLPMEVHSEQGCDVISRLKVRINEVYTALNMIDYGLD
                     NLPGGPLMVEGFTYIPHRFALGFAEAPRGDDIHWSMTGDNQKLYRWRCRAATYANWPT
                     LRYMLRGNTVSDAPLIIGSLDPCYSCTDRMTVVDVRKKKSKVVPYKELERYSIERKNS
                     PLK"
     gene            complement(30234..31157)
                     /gene="hycD"
                     /locus_tag="ACHOSC_RS03165"
                     /old_locus_tag="pgaptmp_000633"
     CDS             complement(30234..31157)
                     /gene="hycD"
                     /locus_tag="ACHOSC_RS03165"
                     /old_locus_tag="pgaptmp_000633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase subunit HycD"
                     /protein_id="HGU4546630.1"
                     /translation="MSVLYPLIQALVLFAVAPLLSGITRVARARLHNRRGPGVLQEYR
                     DIIKLLGRQSVGPDASGWVFRLTPYVMVGVMLTIATALPVVTVGSPLPQLGDLITLLY
                     LFAIARFFFAISGLDTGSPFTAIGASREAMLGVLVEPMLLLGLWVAAQVAGSTNISNI
                     TDTVYHWPLSQSIPLVLALCACAFATFIEMGKLPFDLAEAEQELQEGPLSEYSGSGFG
                     VMKWGISLKQLVVLQMFVGVFIPWGQMETFTAGGLLLALVIAIVKLVVGVLVIALFEN
                     SMARLRLDITPRITWAGFGFAFLAFVSLLAA"
     gene            complement(31160..32986)
                     /gene="hycC"
                     /locus_tag="ACHOSC_RS03170"
                     /old_locus_tag="pgaptmp_000634"
     CDS             complement(31160..32986)
                     /gene="hycC"
                     /locus_tag="ACHOSC_RS03170"
                     /old_locus_tag="pgaptmp_000634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_process: GO:0042773 - ATP synthesis coupled electron
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase subunit 3"
                     /protein_id="HGU4546631.1"
                     /translation="MSAISLINSGVAWFVAAAVLAFLFSFQKTLSGWIAGIGGAVGSL
                     YTAAAGFTVLTGAVGVSGALSLVSYDVQISPLNAIWLITLGLCGLFVSLYNIDWHRHA
                     QVKCNGLQINMLMAAAVCAVIASNLGMFVVMAEIMALCAVFLTSNSKEGKLWFALGRL
                     GTLLLAIACWLLWQRYGTLDLRLLDMRMQQLPLGSDIWLLGVIGFGLLAGIIPLHGWV
                     PQAHANASAPAAALFSTVVMKIGLLGILTLSLLGGNAPLWWGIALLVLGMITAFVGGL
                     YALMEHNIQRLLAYHTLENIGIILLGLGAGVTGIALEQPALIALGLVGGLYHLLNHSL
                     FKSVLFLGAGSVWFRTGHRDIEKLGGIGKKMPVISIAMLVGLMAMAALPPLNGFAGEW
                     VIYQSFFKLSNSGAFVARLLGPLLAVGLAITGALAVMCMAKVYGVTFLGAPRTKEAEN
                     ATCAPLLMRVSVVALAICCVIGGVAAPWLLPMLSAAVPLPLEPANTTVSQPMITLLLI
                     ACPLLPFIIMAICKGDRLPSRSRGAAWVCGYDHEKSMVITAHGFAMPVKQAFAPVLKL
                     RKWLNPVSLVPGWQCEGSALLFRRMALVELAVLVVIIVSRGA"
     gene            complement(32983..33594)
                     /gene="hycB"
                     /locus_tag="ACHOSC_RS03175"
                     /old_locus_tag="pgaptmp_000635"
     CDS             complement(32983..33594)
                     /gene="hycB"
                     /locus_tag="ACHOSC_RS03175"
                     /old_locus_tag="pgaptmp_000635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase subunit HycB"
                     /protein_id="HGU4546632.1"
                     /translation="MNRFVIADSTLCIGCHTCEAACSETHRQHGLQSMPRLRVMLNEK
                     ESAPQLCHHCEDAPCAVVCPVNAITRVDGAVQLNESLCVSCKLCGIACPFGAIEFSGS
                     RPLDIPANANTPKAPPAPPAPARVSTLLDWVPGIRAIAVKCDLCSFDEQGPACVRMCP
                     TKALHLVDNTDIARVSKRKRELTFNTDFGDLTLFQQAQSGEAK"
     gene            complement(33719..34180)
                     /gene="hycA"
                     /locus_tag="ACHOSC_RS03180"
                     /old_locus_tag="pgaptmp_000636"
     CDS             complement(33719..34180)
                     /gene="hycA"
                     /locus_tag="ACHOSC_RS03180"
                     /old_locus_tag="pgaptmp_000636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase regulator HycA"
                     /protein_id="HGU4546633.1"
                     /translation="MTIWEISEKADYIAQRHRRLQDQWHIYCNSLVQGITLSKARLHH
                     AMSCAPDKELCFVLFEHFRIYVTLADGFNSHTIEYYVETKDGEDKQRIAQAQLSIDGM
                     IDGKVNIRDREQVLEHYLEKIAGVYDSLYTAIENNVPVNLSQLVKGQSPAA"
     gene            34392..34742
                     /gene="hypA"
                     /locus_tag="ACHOSC_RS03185"
                     /old_locus_tag="pgaptmp_000637"
     CDS             34392..34742
                     /gene="hypA"
                     /locus_tag="ACHOSC_RS03185"
                     /old_locus_tag="pgaptmp_000637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008901 - ferredoxin hydrogenase activity
                     [Evidence IEA];
                     GO_function: GO:0016151 - nickel cation binding [Evidence
                     IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase maturation nickel metallochaperone
                     HypA"
                     /protein_id="HGU4546634.1"
                     /translation="MHEITLCQRALELIEQQAAKHGAKRVTGVWLKIGAFSCVETSSL
                     AFCFDLVCRGSVAEGCKLHLEEQEAECWCETCQQYVTLLTQRVRRCPQCHGDMLQIVA
                     DDGLQIRRIEIDQE"
     gene            34746..35618
                     /gene="hypB"
                     /locus_tag="ACHOSC_RS03190"
                     /old_locus_tag="pgaptmp_000638"
     CDS             34746..35618
                     /gene="hypB"
                     /locus_tag="ACHOSC_RS03190"
                     /old_locus_tag="pgaptmp_000638"
                     /EC_number="3.6.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0016151 - nickel cation binding [Evidence
                     IEA];
                     GO_process: GO:0051604 - protein maturation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase nickel incorporation protein HypB"
                     /protein_id="HGU4546635.1"
                     /translation="MCTTCGCGEGNLYIEGDEHNPHSAFRSAPFAPAARPKMKITGIK
                     APEFTPSQTEEGDLHYGHGEAGTHAPGMSQRRMLEVEIDVLDKNNRLAERNRARFAAR
                     KQLVLNLVSSPGSGKTTLLTETLMRLKDSVPCAVIEGDQQTVNDAARIRATGTPAIQV
                     NTGKGCHLDAQMIADAAPRLPLDDNGILFIENVGNLVCPASFDLGEKHKVAVLSVTEG
                     EDKPLKYPHMFAAASLMLLNKVDLLPYLNFDVEKCLACAREVNPEIEIILISATSGEG
                     MDQWLNWLETQRCA"
     gene            35609..35881
                     /gene="hypC"
                     /locus_tag="ACHOSC_RS03195"
                     /old_locus_tag="pgaptmp_000639"
     CDS             35609..35881
                     /gene="hypC"
                     /locus_tag="ACHOSC_RS03195"
                     /old_locus_tag="pgaptmp_000639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 3 maturation protein HypC"
                     /protein_id="HGU4546636.1"
                     /translation="MCIGVPGQIRTIDGNQAKVDVCGIQRDVDLTLVGSCDENGQPRV
                     GQWVLVHVGFAMSVINEAEARDTLDALQNMFDVEPDVGALLYGEEK"
     gene            35881..37002
                     /gene="hypD"
                     /locus_tag="ACHOSC_RS03200"
                     /old_locus_tag="pgaptmp_000640"
     CDS             35881..37002
                     /gene="hypD"
                     /locus_tag="ACHOSC_RS03200"
                     /old_locus_tag="pgaptmp_000640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016530 - metallochaperone activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase formation protein HypD"
                     /protein_id="HGU4546637.1"
                     /translation="MRFVDEYRAPEQVMQLIEHLRERASHLSYTAERPLRIMEVCGGH
                     THAIFKFGLDQLLPENVEFIHGPGCPVCVLPMGRIDTCVEIASHPEVIFCTFGDAMRV
                     PGKQGSLLQAKARGADVRIVYSPMDALKLAQENPTRKVVFFGLGFETTMPTTAITLQQ
                     AKARDVQNFYFFCQHITLIPTLRSLLEQPDNGIDAFLAPGHVSMVIGTDAYNFIASDF
                     HRPLVVAGFEPLDLLQGVVMLVEQKIAAHSKVENQYRRVVPDAGNLLAQQAIADVFCV
                     NGDSEWRGLGVIESSGVHLTPDYQRFDAEAHFRPAPQQVCDDPRARCGEVLTGKCKPH
                     QCPLFGNTCNPQTAFGALMVSSEGACAAWYQYRQQESEA"
     gene            36999..38009
                     /gene="hypE"
                     /locus_tag="ACHOSC_RS03205"
                     /old_locus_tag="pgaptmp_000641"
     CDS             36999..38009
                     /gene="hypE"
                     /locus_tag="ACHOSC_RS03205"
                     /old_locus_tag="pgaptmp_000641"
                     /EC_number="4.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417210.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase maturation carbamoyl dehydratase
                     HypE"
                     /protein_id="HGU4546638.1"
                     /translation="MNNIQLAHGSGGQAMQQLINSLFMEAFANPWLAEQEDQARLDLA
                     QLVAEGDRLAFSTDSYVIDPLFFPGGNIGKLAICGTANDVAVSGAIPRYLSCGFILEE
                     GLPMETLKAVVTSMAETARTAGIAIVTGDTKVVQRGAADKLFINTAGMGAIPANIHWG
                     AQTLTAGDVLLVSGTLGDHGATILNLREQLGLDGELVSDCAVLTPLIQTLRDIPGVKA
                     LRDATRGGVNAVVHEFAAACGCGIEISEAALPVKPAVRGVCELLGLDALNFANEGKLV
                     IAVERNAAEQVLAALHSHPLGKDAALIGEVVERKGVRLAGLYGVKRTLDLPHAEPLPR
                     IC"
     gene            38083..40151
                     /gene="flhA"
                     /locus_tag="ACHOSC_RS03210"
                     /old_locus_tag="pgaptmp_000642"
                     /pseudo
     CDS             38083..40151
                     /gene="flhA"
                     /locus_tag="ACHOSC_RS03210"
                     /old_locus_tag="pgaptmp_000642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311614.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase transcriptional activator
                     FlhA"
     gene            complement(40188..40541)
                     /gene="ygbA"
                     /locus_tag="ACHOSC_RS03215"
                     /old_locus_tag="pgaptmp_000643"
     CDS             complement(40188..40541)
                     /gene="ygbA"
                     /locus_tag="ACHOSC_RS03215"
                     /old_locus_tag="pgaptmp_000643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrous oxide-stimulated promoter family
                     protein"
                     /protein_id="HGU4546639.1"
                     /translation="MSGKRISREKLTIKKMIDLYQAKCPQASAEPEHYEALFVYAQKR
                     LDKCVFGEEKPACKQCPVHCYQPAKREEMKQIMRWAGPRMLWRHPILTVRHLIDDKRP
                     VPELPEKYRPKKPRE"
BASE COUNT         9531 a        11191 c        10539 g         9282 t
ORIGIN      
        1 cgctactaaa gccatatcaa gtaagagatg gtgcatccgg gaggattact cgccttgcgg
       61 ctcgcccttc gggccgttgc taaagcaacg ttatcatccc tggtgcctgc ggttaattcc
      121 gctacctctt cgataccttc attgctgaag atttcggaga atatggtgca tccgggagga
      181 ttcgaacctc cgaccgctcg gttcgtagcc gagtactcta tccagctgag ctacggatgc
      241 atcgggaaaa tttattttac tgctgatatt gataccgcta ctaaagccat atcaagtaag
      301 agatggtgca tccgggagga ttcgaacctc cgaccgctcg gttcgtagcc gagtactcta
      361 tccagctgag ctacggatgc aaatggcggt gaggcgggga ttcgaacccc ggatgcagct
      421 tttgaccgca tactccctta gcaggggagc gccttcagcc tctcggccac ctcaccacac
      481 gcctcttacg agtgcttcga agaacttgtt tatcgctcat cgtcgctgcg tggcgcacat
      541 attactttct gagacttata agtcaaacaa tttttcctac acttttatcg tttgcacact
      601 tcacgttcaa ttagtctgta aaaacgtcaa aaagagtgtt ttatcaacag aaaaatggag
      661 gtctgacaga tagtagtaat gcaaaaaaat ggagacttaa gttgaatgaa cgggagtaaa
      721 gcgaaaagac tatagagtga aggagaaatt ttgagggtgc gtctcaccga taaagatgag
      781 acgcggaaag attagtaact ggactgctgg gatttttcag cctggatacg ctggtagatc
      841 tcttcacggt gaacagaaac ttccttcggg gcatttacgc caatacgtac ctggttgccc
      901 tttaccccta aaactgtcac ggtgacctca tccccaatca tgagggtctc accaactcga
      961 cgagtcagaa tcagcattct ttgctccttg aaagattaaa agagtcgggt ctctctgtat
     1021 cccggcatta tccatcatat aacgccaaaa agtaagcaat gacaaacaca ttacatgtaa
     1081 gcagtcatgg cattacattc tgttaaacct aagtttagcc gatatacaca acttcaacct
     1141 gactttatcg ttgtcgatag cgttaatgcg aatgccgtga agcgagtcca cggcattgcc
     1201 tgacgcttat attattgcaa tttcgcgctg acccagcctt tcacactggc taacgctgca
     1261 ggtaaggccg cagcatccgt accaccggct tgcgccatgt caggacgtcc accacccttg
     1321 ccgcccacct gctgagcgac cataccaatc agttcccctg ctttcacacg atctgtgacg
     1381 tccttagata cgcctgcaat cagagaaacc ttaccttcgg ctaccgttgc cagcacgata
     1441 attgtcgacc ccagctgatt ttttaaatcg tcaaccatgg tacgcaacat tttcggctca
     1501 acaccgctaa gctcgctaac caacagctta acaccattaa catcaattgc cttactggaa
     1561 agatttgcgc tctcctgtgc ggcagcttgt tctttaagct gttgtagttc tttttctaac
     1621 tgacgcgtac gttccagtac tgagcgcact ttatccgcca gattattgct atcgcctttc
     1681 agcagatgcg cgacttcgct taagcgatcg ctgtctgcat gaacggtggt gatagcgcct
     1741 tctccggtta ccgcttcgat acgacgaacg cctgcagcag tacccgattc agagatgatg
     1801 cggaacagac caatatcacc agtgcggctg gcgtgagtac cgccacacag ctcggtggag
     1861 aaatcgccca tgctcagcac gcgtacacgc tcatcatact tctcgccgaa cagcgccatc
     1921 gcacctttcg ctttcgccgc ttcgagatcc atgatgttgg tttcgatcgg caaattgcga
     1981 cgaatctgtg cgttcaccag gtcttcgacc gcacgaatct cttccggttt catcgcttcg
     2041 ttgtgtgaga agtcgaagcg cagcaccttg tcgttaacca gtgaaccttt ctgcgataca
     2101 tgagtcccca gaacctggcg cagcgcagcg tgcatcaggt gcgttgcgga gtgattcaga
     2161 cgaatacggg cgcgacgagc ctcatcaaca tcagcctgca cagcgtcgcc cactttcaga
     2221 gaacccgcag caagtttacc gatgtgacca atcgcctggc cgtatttctg cgtatcttcc
     2281 accgcgaagg agaagttagc gcctttcagt tcgcctttat caccaacctg accgccggat
     2341 tccgcataga atggtgtttg atccagcacg accacagctt cctggcctgc attgattgca
     2401 tcaaccgctt taccatcaac aaacagcgcg gtcactttgc cgttcagttc cagatggtca
     2461 tagcctttaa attcagatgc actatcaaca cggatcattg cgttgtaatc ggcaccaaag
     2521 ccgctggctt cgcgcgcgcg acgacgctgc tcttccattg ctgcttcaaa accagcttcg
     2581 tcaactttga tgttgcgctc acgacaaaca tcagccgtca ggtcaaccgg gaagccatag
     2641 gtgtcgtaca gacggaaagc cgtttcacca tccagcgtat caccagaaag ttttgccagc
     2701 tcttcatcca gcaacgccag accgcgctcc agagtacggg caaactgctc ttcttcagtc
     2761 ttcagcacct gctcaacctg cgcctgctgg cgtttcaggt cttcacccgc agagcccata
     2821 acgtcgatca gcggaccaac cagtttgtag aagaaggttt ctttcgcgcc gagcatattg
     2881 ccgtgacgca ctgcgcgacg aatgatacga cgcagtacat aaccacggtt ttcattggac
     2941 ggcattacgc catccgcgat caggaacgca caagaacgaa tgtggtcagc gattacgcgc
     3001 agcgatttat tgctcagatc ggtcgcgcca gtgactttcg ctaccgcctg gatcaacgtg
     3061 cggaacaggt cgatgtcata gttagagtta acgtgttgca gcaccgcagc aatacgctcc
     3121 agacccatac cggtatctac agacggcttc ggcagcggtt ccatcgtgcc atctgcctgg
     3181 cggttgaact gcatgaagac gatgttccag atctcaatgt agcggtcgcc gtcttcttcc
     3241 ggacttcccg gaggaccacc ccaaatgtgg tcgccgtgat cgtagaagat ttcggtgcac
     3301 gggccgcacg gaccagtgtc acccatctgc cagaagttgt cagatgcgta tggcgcacct
     3361 ttgttatcgc cgatgcgaat aatacgttcg cgcgggatcc cgacttcttt ttcccagatt
     3421 tcgtaggctt cgtcgtcgct ttcatagacg gtaacccaca gacgctcttt cggcagggca
     3481 aaccattttt cgctggtcag cagttcccat gcaaactgaa tggcatcgtg tttgaaatag
     3541 tcgccgaagc tgaagttgcc cagcatttcg aagaaggtat ggtgacgcgc ggtgtaaccg
     3601 acgttttcca ggtcgttgtg tttaccaccc gcacgcacgc agcgttggga agtggtagcg
     3661 cgggaataat tacgcttgtc gagcccaagg aacacatcct tgaactggtt catcccggcg
     3721 ttggtaaaca acaaagttgg gtcgttatgg ggtaccaggg agctgctggc aactacctga
     3781 tgtcccttac tatggaaaaa gtcgagaaac gcctgacgga tctcagcggt gctcttgctc
     3841 ataattatcc tgaaatcaag ctaacgaaat atcgccacca gctccagcgt gtcttaaccg
     3901 ccgggctggt aactgaaaag tgggaataag ataagttttc ttgactggga agtaaaatac
     3961 cgtatgcgtt cagtcggcaa aatttcgcca aatatcctgg atatcttcca tcagatagcc
     4021 acgatagagc agaaaacgct ggatcttaac tttttctgaa aagacagttg gcaaaggttc
     4081 accatatttt cgcgtcgcct gatcgcgcgc cagtgcgcac cagtcgatgt cacattcacg
     4141 catcgctttt tctgtcgctt cgcgggaaat acctttctga ttcagttcct gacgaatacg
     4201 tgcaggtcca taacctttgc ggctacggct ggcgataaag cgcgcaacaa atcggctgtc
     4261 atcgagatag ccatgttcat ggcaccaggc aataacgcgc tcgtaatctt ctgccgtagc
     4321 atcaatctct tctgggccat ttttgcccat aatcggtgcc acgagtttac gtcgcagttc
     4381 ttgctcactg tgatcgcgca ccgccagaat gcgtaccgca cgatccaaca ggcgagcata
     4441 tgccgggcga cgggacgttg attctgtcat ggcatatcct tacaacttaa aaaagcaaaa
     4501 gggccgcaga tgcgacccag gttttgctga taaacagatt taatagtcaa tgagtgtgca
     4561 gaatgttccc gcaggcccaa ttcgttagag gatcgaggtg agagtaattt ttcacgccag
     4621 tgctggcctg ttcccctccc cctaaccctc tccccaaagg ggcgagggga ccgtccgtgc
     4681 acacattatc tattatcatc aataacgaac gattaaaaat cttcgttagt ttctgctacg
     4741 ccttcgctgt catctacaga gaaatccggc gttgagttcg ggttgctcag cagcaactca
     4801 cgtactttct tctcgatctc tttcgcggtt tccgggttat ctttcagcca ggcagttgca
     4861 ttcgctttac cctgaccgat cttctcacct ttgtagctgt accacgcgcc tgctttctcg
     4921 atcagcttct cttttacgcc caggtcaacc agttcgccgt agaagttgat accttcgccg
     4981 tagaggatct gaaattcagc ctgtttaaac ggtgcagcga ttttgttctt caccactttc
     5041 acgcgggttt cgctacccac cacgttttcg ccctctttca ccgcgccgat acgacggatg
     5101 tcgagacgaa cagaggcgta gaatttcagc gcgttaccac cggtagtggt ttccgggtta
     5161 ccgaacatca caccaatttt catacggatc tggttgatga agatcagcag cgtgttggac
     5221 tgcttcaggt tacccgccag cttacgcatc gcctggctca tcatacgtgc cgcaaggccc
     5281 atgtgagagt cgccgatttc gccttcgatt tccgctttcg gcgtcagtgc cgccacggag
     5341 tcaacgacga taacgtctac tgcgccagaa cgcgccaggg cgtcacagat ttccagtgcc
     5401 tgctcgccgg tgtccggctg ggagcacagc aggttatcga tatcgacgcc cagtttacgt
     5461 gcgtagattg ggtccagcgc gtgttcagca tcgataaacg cacaggtttt accttcacgc
     5521 tgcgctgcgg cgatcacctg cagcgtcagc gtggttttac cggaagattc cggtccgtag
     5581 atttcgacga tacggcccat cggcagacca cctgccccaa gcgcgatatc cagtgaaagc
     5641 gaaccggtag agatggtttc cacatccatg gaacggtctt cacccaggcg catgatggag
     5701 cctttaccaa attgtttctc aatctggccc agtgctgccg ccaacgcttt ctgtttgttt
     5761 tcgtcgatag ccatttttac tcctgtcatg ccgggtaata ccggatagtc aatatgttct
     5821 gttgaagcaa ttatactgta tgctcataca gtatcaagtg ttttgtagaa attgttgcca
     5881 caaggtctgc aatgcatacg cagtagcctg acgacgcacc gcatcacggt caccgctgaa
     5941 gcattcccgc cgggtaatgc cttcaccgcg ggcagtggca aaagcaaacc agacggtgcc
     6001 gacaggcttc tcttcactgc cgccatccgg cccggcgata ccactaatag acacggcata
     6061 atcagcacga gccgctttca gtgcacctat cgccatttcc accacgacgg gttcactcac
     6121 cgcgccatgc tgcgccagcg tctcttcgcg tacgccgatc atctgcgctt tggcttcgtt
     6181 actgtaggtg acaaatccgc gttcaaacca ggcggagcta ccggcaatat cggtaatcac
     6241 tttcgctacc caaccaccgg tacaagactc ggcagttgtt acggttgcgc cacgggcttt
     6301 cagcgcctgc ccaacctgtt cacttaactg catcagttca ctgtcagtca tcacaacctc
     6361 tgtcagtcaa aaatttatgc cggacaagat agcactttcg tcaggaagat ggtgttggcg
     6421 ttgcaatttt acgaggagga ttcagaaaaa agctgattag ccagagggaa gctcacgccc
     6481 ccctcttgca aatagttact gcacgcgcgc cagcgccacg gcttgtccta actgccagac
     6541 cgccattgcg taatgggtgc tgtggttata acgggtaatg gtgtagaagt tcggcagacc
     6601 gtaccagtac tggtaaccgg taccgacatc cagacgcagc aggctggctt gttgatggtt
     6661 gcccagcggc tgctgtggcg ttaaacctgc cgtggcaagc tgcgaaatgc tgtacctggt
     6721 tttgaagcca tttggcaagc ctggagcctg accgtttgcc attaccgcga cctgatcgcc
     6781 tttcacccag ccgtgcgctt tgaaatagtt cgccacgcta ccgatcgcat caaccggatc
     6841 ccacaggttg atatgcccgt cgccgctgaa atctaccgca tattgtttgt aagacgacgg
     6901 cataaactgt acgtagccca tcgccccggc aaaggaacct ttcagattga gcggatcgtc
     6961 ctgctcgtcg cgcgccatca acaggaaggt ttccagctcg ccagagaaat actccgcgcg
     7021 gcgtgggtag ttaaatgaca gcgttgccag cgcatcgagg atgcgagttt tccccatcac
     7081 gcgcccccag cgggtttcaa cgccgataat cccgacgata atttccggcg gtacgccata
     7141 cacctgccac gcgcgattca acgcatcttc atactgattc cagaaaacca caccgttctg
     7201 cacgttgtcc ggcgtaataa attttttgcg ataacggagc catgcgccgt tcggacctga
     7261 tggcggtttc accgatgtgg ttggtgcctg gttatccatc agccgcagta ccgaatccag
     7321 acgcttcgcc tgggagagaa tttcctgcaa ctgctgacga tcgaaaccgt gtttgttcac
     7381 cattttgtcg atgaactgct gggcattcgg gttattagcg aaatcgccgc ccatctgcat
     7441 cacattgtgc tgcggctcaa gcaggaagcc gccagacggc gttccggtgg tcgtttcagt
     7501 ctcagtaggt tttggcttgc tgctacaggc ggcaagcaac acaaaaaggg gaagcaatgt
     7561 tacataacga cgcttgaaca tgaggggtcc atttaacaga ttcaaccagg ggcaagtatg
     7621 gtaaagcatc acgccccaca caaggaagcg gtagtcactg cccgatacgg actttacata
     7681 actcaactca ttcccctcgc tatcctttta ctcaaacttt caaattaaaa tatttatctt
     7741 tcattttgag atcaaaataa cacttttaaa tctttcaatc tgattagatt aggttgccgt
     7801 ttggtaataa aacaataaat cctgaaggag agaacaatga tagaaaccat tactcatggt
     7861 gcagagtggt ttatcgggct gttccaaaag ggcggagagg tgtttaccgg gatggtgacc
     7921 ggcattcttc cgctgttgat tagcctgctg gttatcatga acgcactgat taattttatc
     7981 ggtcagcatc gtattgaacg ttttgctcaa cgttgcgccg gtaaccctgt ttcccgttac
     8041 ctactgttac cgtgcattgg cacgtttgtc ttttgcaatc cgatgacctt aagcctgggt
     8101 cgctttatgc cggaaaagta caaacccagc tactacgcgg cggcctctta tagctgccac
     8161 tcaatgaatg gcctcttccc ccatatcaac cctggcgaac tgtttgttta tcttggcatt
     8221 gccagcggtc tgacaacgct gaacctgcca cttggcccac tggcggtgag ttatctgctg
     8281 gttggtctgg tcaccaattt cttccgcggc tgggtgaccg atctgaccac cgccattttt
     8341 gagaaaaaga tgggcattca acttgaacaa aaagttcacc tggcaggagc aacatcatga
     8401 cgcatattcg gatcgaaaaa ggaacgggtg gctggggcgg cccgcttgag ctgaaagcca
     8461 cgccgggaaa aaaaatcgtc tatatcaccg ccggtacccg gcctgcgatt gttgacaaac
     8521 tggcacagct tactggctgg caggctattg acggatttag agaaggtgaa cccgcggagg
     8581 cggaaattgg tgtcgcggta atcgactgtg gcggcacatt acgctgcggc atctatccga
     8641 aacgacgtat tcccaccatt aatatccact cgacgggcaa gtccggtccg ctggcgcagt
     8701 acattgtgga agatatttat gtctctggcg taaaagaaga aaacatcact gtagtgggtg
     8761 atgcgacacc acaaccctct tccgtgggcc gtgactatga caccagtaag aaaatcaccg
     8821 aacaaagcga tggtttactg gcgaaggtgg gaatgggcat ggggtccgcc gttgcggtgc
     8881 tgtttcaatc tggtcgtgac accatcgaca ctgtattaaa aaccattctg ccgtttatgg
     8941 cattcgtctc ggcgctcatt ggcatcatta tggcttctgg ccttggtgac tggattgccc
     9001 acggtcttgc tccgctggcg agccatccac tgggtctggt catgctggcg ctcatctgct
     9061 ccttcccact gctttcacct ttcctcggcc caggcgcagt tatcgcacag gttatcggcg
     9121 tattgattgg cgtgcagatt ggtctcggca atattccgcc gcatctggct ttaccggcac
     9181 tgtttgccat caacgcgcag gcggcctgcg acttcatccc ggtcggtttg tcgctggcgg
     9241 aagcccgtca ggacacggtt cgcgtcggtg tcccttctgt actggtgagc cgctttttaa
     9301 ccggcgcacc gactgtactg atcgcctggt ttgtctccgg ttttatctat caatagaggc
     9361 tgaaacatga gcgttattta tcagaccacc atcacccgta tcggcgcgag tgccactgac
     9421 gccctcagcg accagatgct catcaccttt cgtgaaggcg cgccagcgga cctcgaagag
     9481 tattgcttca ttcattgcca cggcgagttg aaaggtgcac tccatcccgg tttgcaattt
     9541 acgctcgggc agcatcgcta tccggtgacc gctgttggca gcgtggcgga agacaacctt
     9601 cgcgaactgg gtcatgtcac cctgcgcttc gatggtttaa acgaagcgga atttccgggc
     9661 actgtccatg tggcaggccc tgtacccgac gatatcgcgc cgggatcggt tttgaagttt
     9721 gaatctgtta aggagtaaaa aatgaatcag gttgccgttg tcatcggtgg tgggcaaacc
     9781 ttaggcgcgt tcctgtgcca cggtctggct gccgaggggt atcgcgtcgc ggttgtcgat
     9841 attcagagcg acaaagccgc aaatgtggca caagaaatta acgccgaata tggtgaaagt
     9901 atggcgtacg gttttggtgc tgacgccact agcgagcaaa gcgttctggc gctctctcgt
     9961 ggggtagatg aaatctttgg tcgcgtggat ttgctggtct atagcgccgg aatagcaaaa
    10021 gcagccttta tcagcgactt ccagctcggc gattttgacc gttcgctaca agtgaatctg
    10081 gtgggttatt tcctgtgtgc ccgtgaattt tcgcgtttga tgatccgcga cgggattcag
    10141 ggtcgcatta ttcagatcaa ctcgaaatcc ggcaaagtgg gcagcaaaca caactctggc
    10201 tacagcgcag cgaaatttgg tggcgtcggg ctgactcaat cactggcgct ggatctggcg
    10261 gagtacggca ttacggtgca ttcactgatg ctcggtaacc tgctgaaatc gccgatgttc
    10321 cagtcactgt tgccacaata cgcgaccaag ctgggtatca aaccggatca agtcgagcag
    10381 tattacatcg acaaagtacc gctcaaacgc ggctgcgatt atcaagatgt gctgaatatg
    10441 ctgctgttct acgccagtcc taaggcgtcg tactgcaccg gacagtcgat caatgtcacc
    10501 ggcggtcagg tgatgttctg atcaacagcg gagatccatt aaggatctcc gtgagactat
    10561 agaatgcctg atgcgctacg ctcatcaggc atacaggact tccgccacta cattaaggaa
    10621 aagttatggt atccgcactc atcaccgtcg ccgttatcgc ctggtgtgcg caactggcct
    10681 taggcggctg gcaaatttct cgttttaacc gtgccttcga cacactatgc cagcaagggc
    10741 gggttggcgt gggccgttcc agcgggcgct ttaaaccgcg ggtcgtggtc gccatcgcgc
    10801 tggacgatca gcagcgcatc gtcgacacct tgtttatgaa aggactgacc gtcttcgccc
    10861 gaccgcaaaa aattcccgca attaccggta tgcatgcggg tgatttacag cccgatgtga
    10921 tctttcccca tgatccacta tcacagaatg ctctatcatt ggcgcttaaa ctgaaacgtg
    10981 gataatttcg ttgtgaatgt tacttgcttg cgaagttatc attttgaaac ctaaatcagg
    11041 taatcacgcc catgaaacct cgtcagcgtc aggccgccat tctggagtat ctgcaaaagc
    11101 agggtaaatg ctcggttgaa gaattggcgc aatactttga caccacaggc acaaccattc
    11161 gcaaagatct ggtcattctg gaacatgccg gaaccgtcat tcgtacttat ggcggagtgg
    11221 tgttgaataa agaggaatcc gatccgccta tcgatcataa aacactcatc aacacccaca
    11281 agaaagagct gattgcagaa gctgccgtta gttttatcca tgatggcgat tcgatcattc
    11341 ttgatgctgg cagtaccgtt ttgcagatgg ttcccctgct ctcgcgcttt aataacatca
    11401 cggtgatgac caacagcctg catatcgtca atgcgctatc cgaactggat aacgaacaaa
    11461 ctatcctgat gccaggcgga acgtttcgta aaaaatcggc ctcatttcac ggacagctgg
    11521 cagagaatgc cttcgaacat ttcagtttcg ataaattgtt tatgggcacc gacggcatcg
    11581 atctcaatgc gggcgtaacc accttcaacg aggtttatac cgtcagtaag gcaatgtgca
    11641 atgccgcgcg cgaagtgatt ttgatggcgg actcatcaaa gtttggccgt aaaagcccca
    11701 acgtagtttg cagtcttgaa agcgtcgata agctgattac cgacgcaggt atcgatccgg
    11761 cgtttcgtca ggcgctggaa gagaaaggga tcgatgtgat cataaccgga gagagcaatg
    11821 agtgaagcac tactgaacgc gggacgtcag acgttaatgc tggagttgca ggaagcaagc
    11881 cgtttaccgg aacgtctggg cgatgatttt gttcgcgccg ccaatatcat cctgcactgc
    11941 gaaggcaaag tggtggtttc gggaattggc aaatcgggcc acattggtaa gaaaatcgcc
    12001 gcaacacttg ccagtaccgg cacaccggct ttttttgtcc atccggcaga agcactgcac
    12061 ggcgatctgg ggatgattga aagccgcgat gtgatgctgt ttatctctta ctccggtggc
    12121 gcgaaggaac tggatctgat tattccgcgt ctggaagata aatctatcgc gctgctggcg
    12181 atgaccggca aaccgacgtc accgctgggc ctggcggcaa aagcggtgct ggatatctcc
    12241 gtagaacgcg aagcctgccc gatgcacctt gcgccgacct ccagcaccgt caataccctg
    12301 atgatgggtg acgcgctggc gatggcggtc atgcaggcgc gcggatttaa tgaagaagat
    12361 tttgcccgct cccacccagc cggggcactg ggcgctcgct tgctgaataa agtgcatcat
    12421 ctgatgcgcc gtgacgatgc catcccacag gtggcgttaa ccgccagcgt gatggatgcg
    12481 atgctggaac tcagccgcac cggtctgggg ctggtggcgg tatgtgacgc tcaacaacag
    12541 gtacaaggcg tctttaccga cggcgattta cgtcgctggc tggttggcgg cggcgcactc
    12601 accacgccag ttaatgaagc gatgacgaca ggcggcacca cattacaggc acaaagtcgc
    12661 gccatcgacg ccaaagagat cctgatgaag cgcaaaatca ctgccgcacc ggtggtggat
    12721 gaaaacggca aactcaccgg cgcaattaac ctgcaggatt tctatcaggc cgggattatt
    12781 taatccttca atcccagacg tttcgccagc cgatgcaggt tggcgacgtc ggtttccagc
    12841 atccgcgcgc aggcagccca gttgtgatga ttttgtgcca gtgcctggcg aatggtttca
    12901 cgctggaacg cttctgtcgc ttcacgcagg ttttgcttaa caacgggcac cgccgccgct
    12961 tctggcggcg gcaacgtcac ctcaggaaaa gcaaaatgtt gtgcctcaag aatcacttca
    13021 tcgccgctgc gagtggctct cgccagcact accgcccgat gaatagcatg ttccagttcg
    13081 cgcacgttcc ccggaaaacg atagtgttgc agtaaatttc gcgctccggc acttaatacc
    13141 acgcgggaga gccccagccg caaacgacac tgctcgcaga aataccccgc cagcagaatg
    13201 acatcatcgc cccgctcacg cagcggcggc accgaaagtg gaaacacgct caggcgatga
    13261 aacaaatcgg cgcggaatcg ccctgccagc acctcttcgc gtaagtcgcg gttagtcgcc
    13321 gccagcacgc gcacatcgac ccgcaaacta cggtcatcgc caacgcgctg aatatcgcca
    13381 tactgcaaca ccctcagcag cttggcctgc aatgccaacg acaactcgcc gatctcatcg
    13441 agaaacagag tgccgttatc cgccatttca aacttcccgc tgcggttact gatagcgcca
    13501 gtaaacgctc ctttcacatg cccgaacaac tcactttccg ccacactttc cggcagtgcg
    13561 gcacagttga gatagaccag cggattcacc gcccgtggcg aggcttcatg aatcgctttc
    13621 gccaccagct ccttaccggt tcccgtctca ccgctgatca gaacgttgag atcggacgcc
    13681 gccacaatct caatctcttt tttcaattgc gtcatgcctg gcgacaagcc aatcatctgc
    13741 gtctgtttca ccgcttcaaa cggcgcggca tcgcccggca gcatattctg gctttccagt
    13801 tgttcaatca gcaacgcatt gcttaacgct cccgccgcca gcgcggcaat cagccgtaac
    13861 tcttcgtcgc tgaaaacatc gaactgatcg ggctgcatcc cgtcgagcgt cagtgcgccg
    13921 atcaggtttt gcccggcaaa caatggcaga ccaacgcagg cgtgaacctt cagactctcc
    13981 tgcccaggaa tcaaaccgtc atagggatcg ggcaattcgc tgtctgcagg aaagcgcacc
    14041 acgtccccgg cgcgggcaat cgcttcaagc cgtggatgcc cttccagcgc aaagcgtcta
    14101 ccgagtacat cctttgccag accgtcgatg gcaagcggaa taaactgccg cgaatcgtaa
    14161 cgtagcaacg cagacgcatc gcactccagc acctgacgta gcgtggtgat caggcgctga
    14221 aaacgatcct gatgaccaat cccacgctgc aattcgatgg cgatattcgc cagcacatca
    14281 acggaaaaac tcatctttgc ctcactgtca atttgactat agatattgtc atatcgacca
    14341 tttgattgat agtcattttg actactcatt aatgagcata attttattta tagagtaaaa
    14401 acaatcagat aaaaaactgg cacgcaatct gcaattagca agacatcttt ttagaacacg
    14461 ctgaataaat tgaggttgct atgtctattg tggtgaaaaa taacattcat tgggttggtc
    14521 aacgtgactg ggaagtgcgt gattttcacg gcacggaata taaaacgctg cgcggcagca
    14581 gttacaacag ctacctcatc cgcgaagaaa aaaacgtgct gatcgacacc gtcgaccata
    14641 aattcagccg cgaatttgtg cagaacctgc gtaatgaaat cgatctggcg gatatcgatt
    14701 acatcgtgat taaccatgca gaagaggacc acgctggggc gctgaccgaa ctgatggcgc
    14761 aaattcccga tacgccgatc tactgtactg ccaacgctat cgactcgata aatggtcatc
    14821 accatcatcc ggagtggaat tttaatgtgg tgaaaactgg cgacacgctg gatatcggca
    14881 acggcaaaca gctcattttt gtcgaaacac caatgctgca ctggccggac agcatgatga
    14941 cttacctgac aggcgacgcg gtgctgttca gtaacgatgc tttcggtcag cactactgcg
    15001 acgagcatct gttcaacgat gaagtggatc agacggagct tttcgagcag tgccagcgtt
    15061 actacgccaa tatcctgacg ccgttcagcc gcctggtaac gccgaaaatt accgagatcc
    15121 tgggctttaa cttaccagtc gatatgatag cgacctccca cggcgtggtc tggcgcgata
    15181 acccgacgca aattgtcgag ctgtacctga agtgggccgc tgattatcag gaagacagaa
    15241 tcaccatttt ctacgacacc atgtcgaata acacccgcat gatggctgac gctatcgccc
    15301 agggaattgc ggaaaccgac ccacgcgtgg cggtgaaaat tttcaacgtc gcccgaagcg
    15361 ataaaaacga aatcctgacc aatgtcttcc gctcaaaagg cgtgctggtc ggcacttcga
    15421 cgatgaataa cgtgatgatg ccgaaaatcg ccgggctggt ggaggagatg actggtttac
    15481 gcttccgtaa caaacgcgcc agtgctttcg gctctcacgg ctggagcggc ggtgcggtgg
    15541 atcgtctttc cacgcgcctg caggatgcgg gtttcgaaat gtcgcttagc ctgaaagcca
    15601 aatggcgacc agaccaggac gctctggagt tatgtcgtga acacggtcgc gaaatcgccc
    15661 gtcagtgggc gctcgcgccg ctgccgcaga gcacggtgaa tacggtggtt aaagaagaaa
    15721 cctctgccac cacgacggct gacctcggcc cacggatgca gtgcagcgtc tgccagtgga
    15781 tttacgatcc ggcaaaaggc gagccaatgc aggacgttgc gccaggaacg ccgtggagtg
    15841 aagttccgga taacttcctc tgcccggaat gctccctcgg caaagacgtc tttgaagaac
    15901 tggcatcgga ggcaaaatga gtaacggcat tgtgatcatt ggttcgggct tcgccgcccg
    15961 ccaactggtg aaaaatattc gcaaacagga cgccactatt ccattaaccc tgattgccgc
    16021 cgacagcatg gatgagtaca acaaacctga cctcagccat gttatcagtc aggggcaacg
    16081 tgccgacgac cttacccgcc agacggcggg tgaatttgcc gagcagttta atctgcgcct
    16141 gtttccgcac acctgggtga cggatatcga tgccgaagcc cgtgtggtga aaagccagaa
    16201 taatcagtgg caatacgaca agctagtgct ggcaaccggt gccagcgcct ttgtcccgcc
    16261 agtgcctggg cgtgagttaa tgctgacgtt aaatagtcag caagagtatc gcgcctgtga
    16321 aacgcaactg cgggatgccc gacgcgtgtt gattgtcggt ggtggcttga ttggtagcga
    16381 actggcgatg gatttttgtc gtgcaggcaa agcggtcacg ctaatcgaca acgctgccag
    16441 tattctggcg tcattaatgc caccggaagt aagcagccgc ttgcagcatc ggttgacgga
    16501 gatgggcgtt catctgttgt tgaaatctca gttgcagggg ctggaaaaaa cagattctgg
    16561 cattctggca acgctggacc gccagcgcag tatcgaagtg gatgcggtaa ttgccgccac
    16621 cggactgcgc ccggaaaccg ccctggcacg acgcgccggg ctgacgatta atcgcggcgt
    16681 ttgcgtcgat agttatctgc aaaccagtaa tgccgatatt tacgcgctgg gcgattgcgc
    16741 ggaaattaac ggtcaggtat tgccgttcct ccagccgatt caacttagcg cgatggtgct
    16801 ggcaaaaaat cttctcggca ataacacgcc actgaaacta ccggcgatgc tggtgaaaat
    16861 caaaacgccg gaattaccgc tgcatctggc aggcgaaacc cagcgtcagg atttacgctg
    16921 gcagattaat accgaacgcc agggaatggt ggcgcgcggc gttgacgatg ctgaccagct
    16981 tcgcgccttt gtggtcagtg aggatcggat gaaagaggca tttggattgt tgaaaacgtt
    17041 gccgatgtag gtgggctact gtgcctaaaa tgtcggatgc gacgctggcg cgtcttatcc
    17101 aacctacggg gagcgcatgt gttggtcaca tactgcgttc atgtcgcatc cacctactgt
    17161 gcccaaaatg tcggatgcga cgctggcgcg tcttatccga cctacgggga cgcatgtgta
    17221 ggccggataa ggcgtttacg ccgcatccgg caatggtgtc caaatgcaac acgttttatc
    17281 cgttctggac ttcacccgct aaccaacgcg ccgcagcaat aaccccctgc cccagagaca
    17341 aaccgccatc acccgccggt aaactctgtg gaaagagcaa tgtgaaatca gcgagataat
    17401 gcgccagacg tgcacgcagc aaacggttat gaataacccc gccgctaaat accagcgtag
    17461 tgataccacg catcgtggcc tgctcacgca tcaacgcggc aaaaccctgc gccagcgcat
    17521 catgaaacgc ccacgcgcgt tgattaaccg gtgcctgcca gttcagccac tgctgccaga
    17581 aagtggcgag atccagttga ttgtccacca gcggcattgt caccggatgc gtcactccgt
    17641 ggcacgaagc tgcgagtgcc tccagagcac aagccgcttc accttcataa cttaacgtgg
    17701 ctggcgcaca gcccagtgcc gccgccacgg catcgaacaa ccgcccacac gatgacgcca
    17761 gcggcgcgtt aattccacgc tcaatggccc gcgccagcac gctccagttt tgctgttgca
    17821 cacttgctgt ttcagaataa ttctgccact ccggcacaaa gcgcaggcac tgcgccagca
    17881 ggtttcgcca cggctgcttc gccgccaaat cgccacccgg aagcgccact gcaggcaagc
    17941 cgcccaggtg ctcacattcg cgatagttca cccgcagaca ctcgccgccc cacaaagcgc
    18001 cgttctcccc cataccgata ccatcgagcg tcaaagcaat gacatccccg ccatccagcg
    18061 gccactgatg ctctgccaga cacgccgctg cgtgggcatg atgatgcagc accgtttgcg
    18121 tcggcatatt catttcgctc gcccactggc tggagacata gcccggatgc acgtcatgca
    18181 caacgtattg cggagtgaaa tcgtagatgt tttgcatcag gcgtaacgct tcgcgccact
    18241 gcatctggat gccatcgtca cttaaatcgc ccagatgctg actcaacacc gcttgttcac
    18301 cgcgcaccag gcagaaggtg tttttcagat ccgcgccgag acacagcaca ggcggaacat
    18361 ttttaaagcc cggaggcaaa gccagcgcat ccggcacata cccccgcgaa cggcgcagca
    18421 tttcgccgct ttcgcgcacc accgaatcat ccatccgctg cacgatgtcg cggttatgta
    18481 tcaagaatcc gtcggcaatg ccctgcaaat ccgccagcgc ctgttcgttg ctgatagctg
    18541 gtggtttacc gctcaggttg ccggaggtca tcaccagcgg gcattgcagt tcctgtaaca
    18601 gcaaatgctg gagcgggttc gcaggcaaca ttaccccgac ttcgttaagg tcaggggcga
    18661 tatcatcaca aagctcagga acgtattttt tatccaccag cacaatcggc gcggcgggcg
    18721 tggtaagcaa ctggcgcgca gcgtctggta aaccgtcagc cactggcaac atgactgcca
    18781 gcggtttcgc cgggcggtgt ttgcgctccc gcagtgtcgc caccgcgtta ctgttacgtg
    18841 catcgcaggc aagatgaaat ccgccaatcc ctttgatggc gacaattttg cccattttta
    18901 actgtgcgat agctgcctgt aatgccgcct cttgttccgc atgttcacca tgacttaccc
    18961 attcaagatg cgggccacac tccgggcagg ccaccggctg ggcgtggaag cgacgatcga
    19021 gcgggtcgcg gtactcttta tcacaggctg gacacagcgg aaactccgcc attacggtaa
    19081 acgggcggtc gtaaggcatg gcgcgaataa tggtgaaacg cgggccgcag tgggtacagt
    19141 tgataaacgg ataacgataa cgccgttcgc ctggggtatt catttcggca aggcaagcag
    19201 ggcaagtagc ggcatccggg acaatttgcg tattcatggc acctcccgcg ctctggcgga
    19261 tagtgaactc ggtgggcagt tgtgaccaga taaacggctc acgctcgacg ctatcaatac
    19321 gcgccagtgg cggacagtgc tgatgcaatt gaacaagaaa cgtttccggg tcttccagca
    19381 gccggacttc tacgccatcg ccgtcattac agacatcgcc gtgaagattt aattgctgtg
    19441 ccagctgcca gacaaacgga cgaaaaccga cgccctgcac tttgccacga atacgcagtt
    19501 ggacaccgca agatgtgttt tttgccattg agttattccc gccatcatga attgcgtaac
    19561 ccgtccagcc ggacacgaca gcgtcgcatc cggcagtcac aggtcggcga taccgcccgc
    19621 ttcgtattct acgaatattt ccgggaattc ctttgatgcc agaacagttc tgtaagattt
    19681 ttagaacatc agcgccgtac ggcggcgttt ttctgcgctc agttgttcaa gtttattacg
    19741 atcgacacaa attagcgcat gggtcgggca agccgccata cacgccgggc cgtcttcacg
    19801 atggttgcac aggtcgcatt tattggcttc agctttgtca gcccgtacat tcagccccac
    19861 gccgctgttg cgaatcaccg gacgtaccac cacttccatc gcgccatacg ggcaagccac
    19921 cacgcaggtt ttgcaaccaa tacaacgttc ctgcatcaca tgaacaaacc ctttatcacg
    19981 gctgatagca ccattcgggc agacgttagc gcacggtgca tcttcacact gacggcaaac
    20041 tgtcgccgtg gaaatgttca cacctttaat gacatggata cgcggtaaaa atgtttccgg
    20101 ggtcagcgat gcacagtcct gattttcctg atgagaaacc acgcacgcca cttcacaggt
    20161 acggcaacca atacatttac tcgcgtcagc aatgatgaaa cggttcatca aattctccag
    20221 caatgacagt taatgcgccg atacattcac aaatcatgcc agtttttaat ttactgttat
    20281 ttaaggaaat taatttctgt aatgcaggaa aaacgatgtc atcgacacta gtgacgatga
    20341 catgtgatga caatgtttat cgcgaaaggg caatgagtga gtcgcggcag atcaattttc
    20401 cgctgaaggt tttcggcggt gagaaatccc cgccatcgag cataaaaatc agccgtccaa
    20461 taatttcctg aatcatctca gtcaccggaa tttttacgct ggagagcgcc ggaacgatat
    20521 agggggcaat tgcgatatca tcgaatccga taactgacac ctgctctggc accgctacgc
    20581 cgcgctcgtg caacgctttc atcgcaccta tcgccatatc gtcgttactg gcaactaacg
    20641 cgctaaattt agccccacgt tcgagcaaca tttctacccc ttcggccccg ctggcaggcg
    20701 tccatctacc gttagcgata agtttttcat tgagcgcaat accgtgctgc gccagcgcgt
    20761 ctttatatcc ggcaagacgt tcaacgctgg tgggagaatc catcgaaccg gtaaggaaag
    20821 caatctcctg atgtccggcg tttatcaact ctgccacggc gttaaaactg gtctgtttat
    20881 gatcgcacca gacgctatgg ctgctgtttt tgcgcaggcg gcgattaagc accattatcg
    20941 gctgactgtg cgcgtcaatg atgtcatcga tctcatccac gcttaaaaaa cgcgggtaaa
    21001 tcataatggc gtcgcagcgc aaatccagca gatactgaat cgcctggcgc tcttcttcgg
    21061 cgctgtgttt accatctgcc aatagcaact gccgcccttt ctcttccgcc attcgcgcgg
    21121 catgaaacag taattcacta aaataaatgc cgtggtaaag cgtgttggtc actaccagcc
    21181 ccagcgtctg agtactcttc gccgacaaat tgcgcgccag caagtttggg cggtaaccgc
    21241 tctcttctac cgcctgaaac acgcgatctt tagtctcctg gctgacgtag ccattacctg
    21301 aaagcacgcg ggaaacggtc gcttttgaaa ccccggcgcg cttcgccact tccagcatcg
    21361 tcgtcatcat tttcatccct ttacacgcaa tcaacgcagt gtactgcacc gtttgccgat
    21421 tgtccttgcg caatcggcgg gaaaaatatt caggtgaccg gtttcacaaa tataaaaaat
    21481 gaacaattaa ctctcttgct tatttagtga caactattca tgattttgtg aaaccggttt
    21541 cctaattccg tttcagcatc ggcatttttc cgttacgtcg actgataaca actacatcta
    21601 ccctactgat aacaggataa aatccgatgg ccaaaaatta tgcggcgctg gcacgctcgg
    21661 tgatagcggc actgggcggc gttgataaca tctcggcggt cacgcactgt atgacgcgtt
    21721 tgcgctttgt tatcaaagat gaccagctta tcgacagccc gacgttaaaa accatccccg
    21781 gcgtgctcgg cgtggtacgt agtgacaacc agtgtcaggt gattatcggc aataccgttt
    21841 cacaagcctt tcaggaagtc gtcagcctac tgccgggaga tatgcagccc gcactgcccg
    21901 tgggtaaacc caaactcacg ctgcgtcgca ttggtgcagg gatcctcgat gcgctgatcg
    21961 gcaccatgtc accgctgatc ccggcgatta tcggcggatc gatggtcaaa ctgctggcga
    22021 tgatcctcga gatgagcggc gtgctgacaa aaggatcgcc gaccttaacc attctgaatg
    22081 tgattggtga cggtgctttc ttcttcctgc cgctgatggt cgcggcatct gccgccatca
    22141 aatttaaaac caatatgtcg ctggcgattg ccatcgcggg tgtgctggta catccgagtt
    22201 ttattgaact gatggcgaaa gcggcccagg gtgaacatgt cgaatttgcc ctgattccgg
    22261 tcaccgcggt gaaatacacc tacacagtga tcccggcgct ggtgatgacc tggtgcctgt
    22321 catatatcga acgctgggtg gatcgcatta caccggcggt gaccaaaaac ttcctcaagc
    22381 caatgctgat tgtgttgatt gccgcaccgc tggcaatcct gctgattggc ccgattggta
    22441 tctggatcgg tagcgccatt tcggcgctgg tttacaccat tcatggttat ctgggctggc
    22501 tttcagtcgc cattatgggc gcgctgtggc ctctgctggt aatgaccggg atgcaccgcg
    22561 tctttacgcc aaccatcatt cagaccattg ccgaaaccgg caaagaaggg atggtcatgc
    22621 cgtcagagat cggcgctaac ctgtcgctgg gcggttcatc actggcggtg gcgtggaaaa
    22681 cgaaaaaccc ggaactgcgc cagacggcgc tggctgcggc ggcatcagcc attatggcgg
    22741 ggatttccga accggcgtta tacggcgtgg cgatccgcct gaaacgtccg cttatcgcca
    22801 gtcttatcag cggctttatt tgcggcgcgg ttgccggtat ggcggggctt gccagccact
    22861 caatggcagc gccggggcta tttaccagcg tgcagttctt cgatccggcg aatccaatga
    22921 gcatcgtctg ggtgttcgcg gtcatggcgc tggcggtcat gctgtcgttt atcctcacac
    22981 tgttgctcgg ctttgaggat attcctgttg aggaagcgac tgccgaggcg cgaaagcatc
    23041 agagcgtaca accgaccgtc gccaaagaag taagtcttaa ttgaggatga aaatgtcagt
    23101 atttccagaa ggttttttat ggggcggcgc gcttgccgcc aaccagtctg aaggtgcgtt
    23161 ccgtgaaggt ggcaaagggc tgaccactgt cgatatgatc ccacacggcg agcatcgaat
    23221 ggcggtgaaa ctggggctgg aaaaacgttt tcagttgcgc gatgacgagt tttatcccag
    23281 ccatgaggcg acggattttt atcatcgtta taaagaagat atcgccctga tggcagagat
    23341 gggattcaag gttttccgta cctcaattgc ctggagccgt ctctttccgc agggcgatga
    23401 aatcacgccc aatcagcagg gcattgcttt ttatcgttct gtctttgaag agtgtaaaaa
    23461 gtacggtatc gaaccgctgg tcacgttgtg ccacttcgat gtgccgatgc atctggtcac
    23521 cgaatatggc tcctggcgta accgcaagct ggtggagttt ttcagccgct acgcccggac
    23581 ctgctttgaa gcatttgatg gtctggtgaa atactggctt accttcaatg aaatcaacat
    23641 tatgttgcat agcccgttct ccggcgcggg tctggtgttt gaagaaggtg aaaatcagga
    23701 tcaggtgaaa tatcaggccg cgcatcacca gctggttgcc agtgcgctag ccaccaaaat
    23761 cgcccatgag gttaacccgc aaaatcaggt gggctgtatg ctggcgggcg gtaacttcta
    23821 cccttacagt tgcaagccgg aagatgtctg ggcggcgctg gagaaagacc gggaaaacct
    23881 gttttttatc gatgtgcagg cgcggggcgc gtatccggct tactctgccc gcgtattccg
    23941 cgaaaaaggg gtaaccatcg acaaagcacc gggcgatgat gaaatcctga aaaacaccgt
    24001 cgattttgtc tctttcagct attacgcctc gcgctgcgcc tcggcggaga tgaacgccaa
    24061 caatagcagt gcggcgaacg tagtgaaatc gctgcgtaac ccgtatctgc aagtgagcga
    24121 ctggggctgg ggtattgatc cactcggtct gcgtatcacc atgaacatga tgtacgaccg
    24181 ttatcagaag ccgctgtttc tggtggaaaa cggcctcggc gcaaaagatg aacttgctgc
    24241 caatggcgag attaacgatg actatcgcat cagctattta cgcgaacata tccgcgcaat
    24301 gggcgaagcg attgcagatg gcattccgct gatgggctac accacatggg gctgtattga
    24361 tttagtttcc gcctctacgg gtgaaatgag caaacgctac ggttttgtct acgtagaccg
    24421 tgacgacgca ggcaacggca cgctgacgcg cacacgtaag aaatcgttct ggtggtataa
    24481 aaaagtgatt gccagtaatg gtgaagattt agagtaagca acagtgccgg atgcggcgtg
    24541 aacgccttat ccggcctaca tgtcgcacga accaggaagg cccgataagc gcagcgcatc
    24601 gggcaatctg gggtttggca tcagtcaaaa tcaaagaaac cattccggct taacgccaaa
    24661 tcgggctgaa agtgccttaa tgtgggatat cgtcagggat cgttgtccag acaaaatctg
    24721 actgaccagg gatttagagc cgatttcgtt cttcaaatcc gcataggtca gattatgttg
    24781 atcaatcagc gtgcgaagta acgccacgcc aacaggcatt gccgcaacgg ctttgtcgaa
    24841 ctctgcaaac ttttcgttgt tgttttcata ctctgcaatg cggctcgcca gaaagtcgat
    24901 aagtggattg gtgtcatctt cttcaatcaa atattcgacc agttccagtg cttcacgata
    24961 atcatcttct gagtcactgc ctcccagaaa ggggactgca ttgaccagtt ctcttgttgc
    25021 tttaacagcg cgtgcagcat tagcagtcat tctttgttct ctctgtagta acgtgtcagt
    25081 ttgtcatact ctgcgtgatt cgttatgtgc ttcacaaaga accgcttatt aacaaagttt
    25141 atgtaggcaa taaccctaag attgttcccg ccgacatcca gaacgtacca tttgttgcga
    25201 tatctaaaat tgtcgaggtt gggaaacgcc gttcgcattt cttctggtgt agaaaaatcc
    25261 gtttccttaa taacccgata caatgcctga tgagccagag cgtcgttagg atatttacgc
    25321 gcagactctt caaatggcgc tttagaaatg atgtgcattt ttaagtttta acttccttat
    25381 agtaagtaaa cagccacatc tgcgatgttc tatccttatg tgttcggaaa ctatagctca
    25441 tcaaatccaa gttcacaata tgtaaacact tgtgcgcgat ataattaccc ccaaaatcct
    25501 catagccact ctgcaaaaag tcagttctgg ttcacccctc aagcacatcc ccctttatca
    25561 agcaacgctg cgtctgcgcc agatcttccg tttgcgggtc gcgcccggcg atgggtttga
    25621 ggacgagttt acggttgggg gtataagtaa aacgcgtgag cgcgggatag cttaacttgc
    25681 caaaccagcc gtgaacacgc tgccatttgg cgtcttttac ctgatacaga ctggttatcc
    25741 agtaaccaga attaaactgc atatccagca attgcgtgtg accattgcct tgcaaatcca
    25801 gcaaatcatc tacgccagtt ttgcttgcgg tgtagaatcc cctgggttca aagacacagg
    25861 gacggccttc gcgcgtgaac gtcataagaa ttaaatgtgc ataaggagcc agaccattac
    25921 cggtacttgg taaccagata accagatcct gaatgccgtt acgatcaaga tcggcgcgat
    25981 agagttgccc tttgcccgct atgtttgcca gcatacccac aggcacagtc caggatttac
    26041 cggcgcggtc tttagcggtt aattgccagt aatcttcttc atttggcatc ttctcaagcg
    26101 tcacgccagc aacgcccact ttcctaaaat gccaggtttg ctcggcatca taaggcatgg
    26161 tcaggagagg tttcagcgcc cgcttttgcg cgtattcgct gtcactcatt ggcagatatt
    26221 cggtaaagct ggtggcctgt gcaggcagca ctaagctcaa taaaaaagcg cacatccctg
    26281 ttctgatgtt caattccctg aattccctga tggagaatta ttcttcttcc accgctaact
    26341 gcgcaaatcc accatttcct tcccagcctt ccagtcgttg ataaacggtt tctaccgcat
    26401 ctttaatcgg ctgggtcatc gggtagtaaa agccgacgat atccggctga atgccgagga
    26461 aaatcacttc accaatgtct tcttttaact ggtcgataag gtaattcaac ggcatgttat
    26521 gggtagtcat cataaacatc tcggcgatat catccgggtc gatgatgcgg atttcgccgg
    26581 ggtttagccc catatccgtg gcgtcgacaa tcagcagtcg tgtcgggcgc agttcacgga
    26641 tggcgacgat gtcgttttcc ggtgcactac cgccgtcaat caccacccag ttacctttcg
    26701 gcgcggcggc gcacttttcc gccagcagcg ggccagcgcc atcatcgccc atcatgctat
    26761 tgccaacaca gagtaaaacg tcagtcacgc agtctcctca ccatcaaata gatggcgttt
    26821 tcctgatgaa tatcatgcag catacttaac agcattgtac tccattcctt ctgctgcgaa
    26881 gtttgcgctg cccgggcttt atcgaatgcg ttggccagca tcggcacatg gttaatgtcg
    26941 atgacgatct cgccatattt cggtacgcct tccattttgc ggcgggcttc gctgcccgcc
    27001 tccagagtgg caatccatgc cagatattca tcccatgggc aggtgagcgc cgcttccagg
    27061 caatcgataa ccccaaggtg gtgaccaatc gccaggctgt aatagaccac ctgctgcgcc
    27121 tcggcgggcg tggcatcgtt ctcatcaata aatttacggc tcagttgact gaacaccacc
    27181 ttttcactca tcggatacgc gcctcttcaa caacatgatt cagatggctg acaatctcgt
    27241 tcagacgcgg atcgttttcc gcttccagcc agcgtgcaac ctgttcttcg ccctgcccta
    27301 actgtgtaag gtaatcatcg gcaatctgac gaccgtaacg ataacccgcc aggcgacgtg
    27361 cttcgcgatc cactttcacg cgcagcggct gcaccatatc gccatgcaga atctcagccg
    27421 gttgttcatc ctgctcaccc gggccacggg cgtgaatttt ctgctccagc aggccgagtg
    27481 ccattgcaaa gccgtacagc gtggcggcag gcgttggcgg gcaacccgga atgtagacat
    27541 ctaccgggac gattttatcc gtaccgcccc acacgcagta gagatcgtgg aaaataccac
    27601 cgctgttacc gcaggcaccg taggagatac aaattttcgg gtccggcgcg gactgccacg
    27661 cacgcagcgc aggggatcgc attgcacggg tgaccgcgcc ggtaaacagt aaaatatccg
    27721 catgacgcgg tgaaggaacg actttaatgc cgaagcgttc tgcatcaaac agcggcgata
    27781 aagtggcgaa aatttcgatt tcgcagccgt tgcagccgcc gcagtccacg cggtaaacat
    27841 aggcagaacg tttgattttt ttcagtaacg acgccttcat gctggcgatg gattcatcca
    27901 ccgtcatcgg gaccggaatg ccgttggcgt cacgggggcc taataaattg ctcatcagat
    27961 ggcctctttc atatggcgag tcagttcaat acggtcggac ggcaccaggc atttctggcg
    28021 cttacattcc gggcaagtct caaagctttc gcggtggttt tccgcgcggc tgtcaccatt
    28081 gtgcttaagc agcgcaatgg cgtagtcgat ctctttctgg acggcgaaag gacgattgca
    28141 gacgcgacag ctgcacagca caaaacggga ctgttgcagg aaatcttctt tcttccacac
    28201 cgccagttcg tactcttgcg acagtttgat cgctgccgtc gggcagactt cttcgcagcg
    28261 tccacagaag atgcagcgcc cgagattaaa ctcccacgcc agctcaccgg tggcgaggtc
    28321 agtttcaacc gttaaggcgt ttgacgggca ggcattgacg caggccgcac agccgatgca
    28381 ctgctgcggg ttctgctccg gcttaccacg gaagttttta tcaaccgcaa tcggctccag
    28441 cggataagac gaggtcgccg tgccggtttt gatgactttt ttgataaagg taaacatggc
    28501 gattccttat ttcagcggcg agtttttacg ctcaatgctg taacgctcga gttctttgta
    28561 cggcaccact ttgctctttt tcttacgcac atcgaccacg gtcatgcggt cggtacagga
    28621 gtagcaaggg tccaggctac cgataatcag cggcgcatcg gaaacggtgt taccgcgcag
    28681 catgtagcgc agggtcggcc agttcgcgta ggtcgcggca cggcagcgcc agcggtacag
    28741 cttctggttg tcgccggtca tgctccagtg gatatcgtcg ccgcgcggcg cttcggcaaa
    28801 gcccagcgcg aaacggtgcg gaatgtaggt aaagccttcc accatcagcg gaccgcccgg
    28861 caggttatcc agaccgtagt cgatcatgtt cagcgcggta taaacttcgt tgatacgcac
    28921 tttcagacgg gaaataacgt cgcagccctg ctcgctgtgg acttccattg gcagcaggcc
    28981 ataaccgaca aacgggtgat cggcgcgagt atcgcgggcg tgaccgctgg cgcgaaccat
    29041 cgggccaacg ttactgaaat cgcgggcgat ttccgggtcc agacgaccaa tgccgacggt
    29101 gcgctgttcc atgttcggcg tactcagcag cacatccacc agctcctgca cttcacgacg
    29161 catctgttgc gccagctggc gggtctggat catgtcatct ttcaacagat cgcgacgaat
    29221 cccgccgatc aggttcaggc cgtaggtttt acgcgcaccg gtgaggatct ctgccatttt
    29281 catggaggtt tcacgcacgc ggaagaactg cataaagccg gagtcaaagc cggtaaagtg
    29341 gcaggcgaga ccgaggttga gcagatgcga gtgcaggcgt tccacctcca gcagaatggc
    29401 gcggatcatc tgcgcacgtt ctggcacctg aatacccatc gcgttttcta ccgacgtggt
    29461 gtaggcggtg ctgtgggcaa agccgcagat cccgcacaca cggtcagaga ggaaggtcac
    29521 ttcgttgtaa cccatacggg tttccgccag tttttccatg ccgcgatgaa cgtagaacag
    29581 acggtagtcg gcgtcgataa tgttttcgcc atcgacgaac agacggaagt ggcccggttc
    29641 gtcagaagtg acgtgcagcg gaccaatcgg cacgacgttg tttttcttgt cgcccagttc
    29701 gttgatgaac tcgtaggttt cagcatcggt agtcggtgcc ggacgctgac gataatccat
    29761 gctgtcttta cgcagcggat aaagttcatc cggccagtca tccggcagca ccagacgacg
    29821 ttcatccggc agaccaaccg gaatcaaacc gtacatatcg cgcacttcgc gctcgcccca
    29881 caccgccgcc ggaacgcgcg gcgtcacgga cggatactcc ggtttgttgg cgtcaacttc
    29941 gacgcgaacc gttacccaac acttggtgcc cttctccatc gacagcacgt aataaacggc
    30001 gtaatgacca ttcagtttgc gttcgtcgtt accaaacagc accgacagcc agccaccctg
    30061 cttgtagtag agaaactcca ccacttccgg caggtagttc accttcacgg tcacggtcag
    30121 ctgatctttg gtctgccagg cgtggtccag cacgacaccc ggaaatgcct cattcagcgc
    30181 ggcgagataa tgttgaccta atttttcttc agacatgctc aaactctctt taatcacgcc
    30241 gccagcaagg agacgaacgc taaaaatgca aagccaaacc cagcccaggt aatgcgcgga
    30301 gtaatatcaa gacgcagacg ggccatgctg ttttcgaaca gcgcgataac caggacgccg
    30361 acgaccagtt tgacgatggc aatcaccagc gccagcagca gcccaccggc ggtgaaggtt
    30421 tccatctgtc cccacgggat aaatacgccg acgaacatct gcagcaccac cagctgtttc
    30481 aggctgatac cccatttcat gacgccaaag ccgctgccgc tgtattcaga gagcgggcct
    30541 tcctgcagct cctgctcggc ttccgccagg tcgaacggca gtttgcccat ttcgataaag
    30601 gtggcgaacg cacaggcaca aagcgccagt accagcggga tgctctggct cagcggccag
    30661 tgataaacgg tgtcggtgat gttgctgatg ttggtggaac cggcaacctg tgcggcaacc
    30721 cacagaccaa gcagcagcat cggttcgacc agcacgccaa gcatcgcttc acggctcgcg
    30781 ccgatagcgg taaacgggct accggtatcc agaccagaaa tggcaaagaa gaaacgcgcg
    30841 atggcaaaga gatacagtaa ggtgattaaa tcacccagtt gcggcagcgg agaaccgacg
    30901 gtcaccaccg gcagcgcagt agcgatagtc agcatgacgc ccaccatcac atacggcgtc
    30961 aggcggaaca cccagccgga ggcatccggg ccaacgctct ggcggcccag cagtttgata
    31021 atgtcacgat actcctgcaa cacacccggc ccacgacggt tatgtaagcg ggctcgcgcc
    31081 acgcgggtaa taccggagag cagcggcgca acggcaaata gcaccagcgc ctgaattaac
    31141 ggatataaaa cactcattct caggctcctc gtgaaacaat aatcaccacc agtaccgcca
    31201 gttcaaccag cgccatccgg cggaacagca acgcactccc ctcgcactgc cagcccggca
    31261 ccagagacac cggattcagc catttgcgta gtttcagcac cggcgcaaac gcctgtttca
    31321 ccggcatggc aaaaccgtga gcggtaatca ccattgattt ttcgtggtcg tagccgcaca
    31381 cccaggccgc gccgcgggaa cgcgatggca aacgatcgcc tttgcaaatc gccataatga
    31441 tgaatggcag cagcggacag gcaatcagca gcaatgtgat cattggttga gaaacggtgg
    31501 tgttagcagg ctccagcggc agaggtacag cagcagacag catcggcagc agccacggcg
    31561 cagcaacacc gccaattacg cagcaaatcg ccagtgccac tacgcttacc ctcatcagga
    31621 gcggcgcaca ggtggcgttt tcggcttctt tggtacgcgg cgcgccgagg aacgtgacgc
    31681 catagacttt cgccatacac atcaccgcca gcgcaccggt aattgccaga cccacagcga
    31741 gcagcggccc cagcagacgg gcaacaaacg cgccgctatt gctcagtttg aagaaggatt
    31801 gatagataac ccattccccg gcaaacccgt tcagcggcgg cagcgcagcc attgccatta
    31861 acccgactaa catggcgatg gagataaccg gcattttctt gccaatacca ccgagttttt
    31921 cgatatcgcg atgaccggta cggaaccaga cgctccccgc cccgaggaac agtacgcttt
    31981 tgaacaggct atggttaagc agatggtaca gaccaccgac caggccaaga gcaatcagcg
    32041 ccggttgttc gagcgcgata cccgttacgc cagcgcccag ccccagcagg ataatgccga
    32101 tattttccag ggtgtggtaa gccagcaggc gctggatgtt gtgctccatc agcgcataca
    32161 gaccaccgac aaacgcggtg atcatgccga gcaccagcag cgcgatcccc caccacagcg
    32221 gtgcattacc gcccagcagt gacagggtta aaatgcccag caggccaatt ttcatgacta
    32281 ccgtagaaaa caacgcggca gctggcgcag aggcgttagc atgtgcctgc ggcacccagc
    32341 cgtgcagcgg aataatcccg gcgagcaggc caaagccaat cacaccgagc agccagatat
    32401 cggaaccgag cggcagctgt tgcatacgca tatccagcag gcgcagatcc agcgtgccat
    32461 aacgctgcca cagcagccag caagcaatcg ccagcagcag agtgccaaga cgccccagcg
    32521 caaaccacag tttgccctct ttgctgttgc tagtgaggaa caccgcgcac agggccatga
    32581 tttcggccat taccacgaac atgccgaggt tgctggcaat gacggcgcag acggcggcag
    32641 ccatcaacat attgatctgc aagccgttgc acttcacctg cgcgtggcga tgccagtcaa
    32701 tgttatagag gctgacaaac agaccgcaaa ggccgagggt aatcagccaa atcgcgttaa
    32761 gcggggagat ttgcacatcg tagcttacca gcgacagcgc accgctcacg ccaaccgcgc
    32821 cagtcagcac agtgaagccc gcggctgccg tatacaggct accaaccgcg ccgccaattc
    32881 cggctatcca gccacttagc gtcttttgaa aagaaaagag aaatgccaga acagcggcgg
    32941 cgacaaacca cgccacgccg ctattgatca gggaaattgc gctcatttag cctctccact
    33001 ttgagcctgc tgaaacaagg tgagatcgcc aaagtccgtg ttaaaggtca gctcacgctt
    33061 acgtttgctg acgcgggcga tatcggtgtt atccaccaga tgcagggctt tagtcgggca
    33121 catccgcacg caggccggac cttgttcatc aaagctacaa aggtcacatt tgacggcgat
    33181 cgcgcgaata cctggcaccc agtcaagcaa tgtgctgaca cgcgccggag caggcggtgc
    33241 cggtggcgct ttcggggtat tggcgtttgc cggaatatcc agcggacggc tgccggaaaa
    33301 ttcaattgcg ccaaacgggc aggcgatgcc gcacagcttg cagcttacgc acaggctttc
    33361 attcaactgc acggccccat caacgcgggt gatggcgtta accgggcaga ccaccgcgca
    33421 gggtgcatct tcacagtggt gacagagctg cggcgcagat tctttttcat tcagcatcac
    33481 tctcaggcgc ggcattgatt gcaggccgtg ctggcgatgc gtctctgaac aggcggcctc
    33541 acaagtgtgg cagccgatac agagcgtgga gtcagcaatt acaaaacgat tcaccaggca
    33601 ttcctcaggt gattgtcatt tttgacgaaa acatgccgat gaaatgtcat tttcgacact
    33661 catcgacacg cccatcccca aacaggcgta acgcctgcaa aacgggcaaa gcctcagctc
    33721 atgctgccgg gctttgtccc tttaccagtt ggcttaaatt caccggcaca ttgttttcaa
    33781 tagcggtgta taagctgtcg taaacgccag cgattttttc gagatagtgt tccagaacct
    33841 gttcgcgatc gcggatgttg accttgccat caatcatgcc gtcaatgctc agttgcgcct
    33901 gcgcaatccg ctgtttgtct tcgccatctt ttgtttcgac gtaatactcg atggtgtggc
    33961 tgttaaagcc atccgccagg gtgacgtaaa tgcgaaaatg ttcaaaaagg acgaaacaga
    34021 gttctttgtc cggcgcgcag ctcatggcgt gatgcaggcg cgctttcgat aacgtgatcc
    34081 cctgaaccag cgaattgcag tagatgtgcc actggtcctg taggcgacga tgccgctgtg
    34141 cgatgtaatc ggctttctcg cttatttccc aaatagtcat tgtcaggtta cccgtttaac
    34201 agagatgcca gctttaagca ttttctgtgc caacttttaa tttattgtta ttaaagagat
    34261 ttttaagcta aagatgaatt tcgtcgccgt gtcgacgtgt catttcgaca tcatcgacat
    34321 tattcaccgc agggataatc aacactggca caattattgc ttgtagctgg caatagttaa
    34381 tgggaggcga tatgcacgaa ataaccctct gccaacgggc actggaattg atcgaacagc
    34441 aggccgcaaa acacggcgca aaacgcgtaa ctggggtctg gctcaaaatt ggcgcatttt
    34501 cttgtgtcga aaccagctct cttgcctttt gttttgatct ggtttgccgc ggcagcgtgg
    34561 cggaaggttg taaactgcac ctcgaagaac aagaggccga atgctggtgt gaaacatgcc
    34621 aacagtatgt gacgctactg acccagcgcg tccgccgctg tccacagtgt catggtgaca
    34681 tgctgcagat tgtggcagac gacggtttac agattcggcg gatagaaata gaccaggagt
    34741 gagcgatgtg tacaacatgc ggttgcggtg aaggcaacct gtatatcgag ggtgatgaac
    34801 ataaccctca ttccgcgttt cgtagcgcgc catttgcccc ggcggcacgc ccgaagatga
    34861 aaatcaccgg cattaaagcg cctgaattta cccccagcca gactgaagaa ggcgacctgc
    34921 attacggtca tggcgaagcg ggcactcacg caccgggcat gagccagcgt cggatgctgg
    34981 aagtcgaaat tgacgtgctg gacaaaaata accgtctggc tgaacgcaac cgcgcgcgct
    35041 ttgctgcccg caagcaactg gtgctcaacc tggtttccag cccaggttcc ggtaaaacca
    35101 ccctgctgac ggaaacctta atgcgcctga aagacagcgt tccgtgcgcg gttattgaag
    35161 gtgaccagca aaccgtgaac gatgccgcac gcattcgcgc taccggcaca ccagcgattc
    35221 aggtgaacac cggtaaaggc tgccatcttg acgcacagat gattgccgac gccgcaccgc
    35281 gtctgccact ggacgataac ggtattctgt ttatcgaaaa cgttggcaac ctcgtatgcc
    35341 cggccagctt cgatctcggt gaaaaacaca aagtggcggt gctttccgtt accgaaggtg
    35401 aagacaaacc gctgaaatat ccgcatatgt ttgccgccgc ctcgctgatg ctgctcaaca
    35461 aagttgacct gttgccgtat ctcaactttg acgttgagaa gtgcctcgcc tgcgcccgcg
    35521 aagtcaatcc agaaattgaa atcatcctta tttccgccac cagcggtgaa gggatggacc
    35581 agtggctgaa ctggctggag acacagcgat gtgcataggc gttcccggcc agatccgcac
    35641 cattgacggc aaccaggcaa aagtcgacgt ctgcggcatt cagcgcgatg tcgatttaac
    35701 gttagtcggc agctgcgatg aaaacggtca gccgcgcgtg ggccagtggg tactggtaca
    35761 cgttggcttt gccatgagcg taattaatga agccgaagca cgcgacactc tcgacgcctt
    35821 acaaaacatg tttgacgttg agccggatgt cggcgcgctg ttgtatggcg aggaaaaata
    35881 atgcgttttg ttgatgaata tcgcgcgccg gaacaggtga tgcagttaat tgagcatctg
    35941 cgcgaacgtg cttcacatct ctcttacacc gccgaacgcc ctctgcggat tatggaagtg
    36001 tgcggtggtc atacccacgc tatttttaaa ttcggcctcg accagttact gcctgaaaac
    36061 gttgagttta tccacggtcc gggttgcccg gtgtgcgtac tgccgatggg tagaatcgac
    36121 acctgcgtgg agattgccag ccatccggaa gtcatcttct gtacctttgg cgacgccatg
    36181 cgcgtaccag ggaaacaggg atcgctgttg caggcaaaag cacgcggtgc cgatgtgcgc
    36241 atcgtttact cgccgatgga tgcgttgaaa ctggcgcagg agaatccaac ccgcaaagtg
    36301 gtgttcttcg gcttaggttt tgaaaccacc atgccgacca ccgccatcac tctgcaacag
    36361 gcgaaagcgc gtgatgtgca gaatttttac ttcttctgcc agcacattac gcttatcccg
    36421 acgctgcgca gtttgctgga acagccggat aacggtatcg acgcgttcct cgcgccgggc
    36481 cacgttagta tggttatcgg cactgatgcc tataatttta tcgccagcga ttttcatcgt
    36541 ccgctggtgg tcgctggttt cgaacccctt gatctactgc aaggcgtggt catgctggtg
    36601 gagcagaaaa tagcggccca cagcaaggta gagaatcagt atcgtcgggt ggtaccggat
    36661 gccggtaacc tgctggcgca acaggcgatt gccgatgtgt tctgtgtcaa cggcgacagc
    36721 gaatggcgcg gcttaggcgt gattgaatct tctggcgtgc acctgacgcc ggattatcaa
    36781 cgattcgatg ccgaagcaca tttccgcccg gcaccgcagc aggtctgcga tgacccgcgc
    36841 gcgcgttgtg gtgaggtatt aacgggcaaa tgtaagccgc atcaatgccc gctgtttggt
    36901 aacacctgta atcctcaaac cgcatttggt gcactgatgg tctcctccga aggagcgtgc
    36961 gccgcgtggt atcagtatcg tcagcaggag agtgaagcgt gaataatatc caactcgccc
    37021 acggcagcgg cggccaggcg atgcagcaat taatcaacag cctgtttatg gaagcctttg
    37081 ccaacccgtg gctggcagag caggaagatc aggcacgtct tgatctggcg cagctggtag
    37141 cagaaggcga tcgtctggcg ttctccaccg acagttacgt cattgacccg ctgttctttc
    37201 ctggcggtaa tatcggcaag ctggcgattt gcggcactgc gaatgacgtt gcggtcagtg
    37261 gcgctattcc gcgctatctc tcctgtggct ttatcctcga agaaggattg ccgatggaga
    37321 cactgaaagc cgtagtgacc agcatggcag aaaccgcccg cacggcaggc attgccatcg
    37381 ttactggcga tactaaagtg gtgcagcgcg gcgcggcaga taaactgttt atcaacaccg
    37441 ctggcatggg cgcaattccg gcgaatattc actggggcgc acagacgcta accgcaggcg
    37501 atgtattact ggttagcggc acgctcggcg accacggggc gactatcctt aacctgcgtg
    37561 agcagctggg gctggatggc gaactggtca gcgactgcgc ggtactgacg ccgcttattc
    37621 agacgctgcg tgacattccc ggcgtgaaag cgctgcgtga tgccacccgt ggtggtgtga
    37681 acgcggtagt tcatgagttc gcggcagcct gcggttgtgg tattgaaatt tcagaagcgg
    37741 cactgccggt taaacctgcc gtgcgcggcg tttgcgaact gctgggactg gacgccctta
    37801 actttgccaa cgaaggcaaa ctggtgatcg ccgttgaacg caacgcggca gagcaagtgc
    37861 tggcagcgtt acattcccat ccactgggaa aagacgcggc gctgattggt gaagtggtgg
    37921 aacgtaaagg tgttcgtctt gccggtctgt atggcgtgaa acgaaccctc gatttaccac
    37981 acgccgaacc gcttccgcgt atatgctaat aaaattctaa atctcctata gttagtcaat
    38041 gaccttttgc accgctttgc ggtgctttcc tggaagaaca aaatgtcata tacaccgatg
    38101 agtgatctcg gacagcaagg gttgttcgac atcactcgga cactattgca gcagcccgat
    38161 ctggcctcgc tgtgtgaggc tctttcgcaa ctggtaaagc gttctgcgct cgccgacaac
    38221 gcggctattg tgttgtggca agcgcagact caacgtgcgt cttattacgc atcgcgtgaa
    38281 aaagacaccc ccattaaata tgaagacgaa actgttctgg cacacggtcc ggtacgccgc
    38341 attttgtcgc gccctgatac gctgcattgc agttacgaag aattttgtga aacctggccg
    38401 cagctgggct ataccccaaa tttggtcact attgcctgat gccactggcg gcggaagggc
    38461 atatttttgg tggctgtgaa tttattcgtt atgacgatcg tccctggagc gaaaaagagt
    38521 tcaatcgtct gcaaacattt acgcagatcg tttctgtcgt caccgaacaa atccagagcc
    38581 gcgtcgtcaa caatgtcgac tatgagttgt tatgccggga gcgcgataac ttccgcatcc
    38641 tggttgccat taccaacgcg gtgctttccc gcctggatat ggacgaactg gtcagcgaag
    38701 tcgccaaaga aatccattac tatttcgaca ttgacgatat cagcattgtc ttacgcagcc
    38761 accgtaaaaa caaactcaac atctactcca ctcactatct tgataaacaa catcccgccc
    38821 acgaacagag cgaagtcgat gaagccggaa ccctcaccga acgcgtgttc aaaagtaaag
    38881 agatgctgct gatcaatctc cacgagcggg acgatttagc cccctatgaa cgcatgttgt
    38941 tcgacacctg gggcaaccag attcaaacct tgtgcctgtt accgctgatg tctggcgaca
    39001 ccatgctggg cgtgctgaaa ctggcgcaat gcgaagagaa agtgtttacc actaccaatc
    39061 tgaatttgct gcgccagatt gccgaacgtg tggcaatcgc tgtcgataac gccctcgcct
    39121 atcaggaaat ccatcgtctg aaagaacggc tggttgatga aaacctcgcc ctgaccgagc
    39181 agctcaacaa tgttgatagt gaatttggcg agattattgg ccgcagcgaa gccatgtaca
    39241 gcgtgcttaa acaagttgaa atggtggcgc aaagtgacag taccgtgctg atcctcggtg
    39301 aaactggcac gggtaaagag ctgattgccc gcgcgatcca taatctcagt gggcgtaata
    39361 atcgccgcat ggtcaaaatg aactgcgcgg cgatgcctgc cggattgctg gaaagcgatc
    39421 tgtttggtca tgagcgtggg gcttttaccg gtgccagcgc ccagcgcatt ggtcgttttg
    39481 aactggcgga taaaagctct ctgttcctcg acgaagtggg cgatatgcca ctggagttac
    39541 agccgaagtt gctgcgtgta ttgcaggaac aggagtttga acgtctcggc agcaacaaaa
    39601 tcattcagac ggacgtacgt ttaattgccg cgactaaccg cgatctgaaa aaaatggtcg
    39661 ccgaccgaga gttccgtagc gatctctatt accgcctgaa cgtattcccg attcacctgc
    39721 cgccactacg cgagcgtccg gaagatattc cgctgctggc gaaagccttt accttcaaaa
    39781 ttgcccgtcg tctggggcgc aatatcgaca gcattcctgc cgagacgctg cgcaccttga
    39841 gcaacatgga gtggccgggt aacgtacgcg aactggaaaa cgtcattgag cgcgcggtat
    39901 tgctaacacg cggtaacgtg ctacagctgt cattgccaga tattgtttta ccggaacctg
    39961 aaacgccgcc tgccgcaacg gttgtcgccc aggagggcga agatgaatat cagttgattg
    40021 tgcgcgtgct gaaagaaacc aacggcgtgg ttgccgggcc taaaggcgct gcgcaacgtc
    40081 tggggctgaa acgcacgacc ctgctgtcac ggatgaagcg gctgggaatt gataaatcgg
    40141 cattgattta actgcaaatt gccggacaga tctgcctgtc cggcatacta ttcacgaggt
    40201 tttttcggac gatatttttc cggcagttct ggcaccggac gcttgtcatc gatgagatga
    40261 cgcacggtta agatcggatg acgccacagc attctcggcc cggcccaacg cataatctgt
    40321 ttcatctctt cacgctttgc aggctggtaa cagtgcaccg gacactgctt acaggctggt
    40381 ttctcttcgc cgaacacaca tttatccagc cgcttttgcg cgtaaacaaa caacgcctcg
    40441 taatgctccg gctccgctga cgcctgcggg catttcgctt gataaagatc gatcattttt
    40501 ttaatcgtca gtttttcacg agagatacgc ttgccggaca tgc
//
LOCUS       DAZKHF020000015        39662 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2201S31241
            SAMN43267800-rid25106413.denovo.015, whole genome shotgun sequence.
ACCESSION   DAZKHF020000015 DAZKHF020000000
VERSION     DAZKHF020000015.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267800
            Sequence Read Archive: SRR30307263
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 39662)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 39662)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 39662)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350581.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350581.2
            Genome Coverage       :: 151x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:21:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,652
            CDSs (total)                      :: 5,557
            Genes (coding)                    :: 5,320
            CDSs (with protein)               :: 5,320
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 237
            CDSs (without protein)            :: 237
            Pseudo Genes (ambiguous residues) :: 0 of 237
            Pseudo Genes (frameshifted)       :: 102 of 237
            Pseudo Genes (incomplete)         :: 130 of 237
            Pseudo Genes (internal stop)      :: 52 of 237
            Pseudo Genes (multiple problems)  :: 41 of 237
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..39662
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267800-rid25106413.denovo.015"
                     /serotype="O 26:H11"
                     /isolate="2201S31241"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-01"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            <1..105
                     /locus_tag="ACHOSC_RS03220"
                     /old_locus_tag="pgaptmp_000644"
     CDS             <1..105
                     /locus_tag="ACHOSC_RS03220"
                     /old_locus_tag="pgaptmp_000644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase"
                     /protein_id="HGU4546640.1"
                     /translation="TTMTITTPTGEGVTSARTFVRLRRCVLVDAFRIV"
     gene            116..952
                     /gene="eutJ"
                     /locus_tag="ACHOSC_RS03225"
                     /old_locus_tag="pgaptmp_000645"
     CDS             116..952
                     /gene="eutJ"
                     /locus_tag="ACHOSC_RS03225"
                     /old_locus_tag="pgaptmp_000645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization protein EutJ"
                     /protein_id="HGU4546641.1"
                     /translation="MAHDEQWLTPRLQTAATLCNQTPAATESPLWLGVDLGTCDVVSM
                     VVDRDGQPVAVCLDWADVVRDGIVWDFFGAVTIVRRHLDTLEQQFGLRFSHAATSFPP
                     GTDPRISINVLESAGLEVSHVLDEPTAVADLLQLDNAGVVDIGGGTTGIAIVKKGKVT
                     YSADEATGGHHISLTLAGNRRISLEEAEQYKRGHGDEIWPAVKPVYEKMADIVARHIE
                     GQGITDLWLAGGSCMQPGVAELFRKQFPALQVHLPQHSLFMTPLAIASSGREKAEGLY
                     AK"
     gene            942..2129
                     /gene="eutG"
                     /locus_tag="ACHOSC_RS03230"
                     /old_locus_tag="pgaptmp_000646"
     CDS             942..2129
                     /gene="eutG"
                     /locus_tag="ACHOSC_RS03230"
                     /old_locus_tag="pgaptmp_000646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311342.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization ethanol dehydrogenase
                     EutG"
                     /protein_id="HGU4546642.1"
                     /translation="MQNELQTALFQAFDTLNLQRVKTFSVPPVTLCGPGSVSSCGQQA
                     QTRGLKHLFVMADSFLHQAGMTAGLTRSLAVKGIAMTLWPCPVGEPCITDVCAAVAQL
                     RESGCDGVIAFGGGSVLDAAKAVALLVTNPDSTLAEMSETSVLQSRLPLIAIPTTAGT
                     GSETTNVTVIIDAVSGRKQVLAHASLMPDVAILDAALTEGVPSHVTAMTGIDALTHAI
                     EAYSALNATPFTDSLAIGAIAMIGKSLPKAVGYGHDLAARESMLLASCMAGMAFSSAG
                     LGLCHAMAHQPGAALHIPHGLANAMLLPTVMEFNRMVCRERFSQIGRALRTKKSDDRD
                     AINAVSELIAEVGIGKRLGDVGATSAHYGAWAQAAQEDICLRSNPRTASLEQIVGLYA
                     AAQ"
     gene            2246..3472
                     /gene="eutH"
                     /locus_tag="ACHOSC_RS03235"
                     /old_locus_tag="pgaptmp_000647"
     CDS             2246..3472
                     /gene="eutH"
                     /locus_tag="ACHOSC_RS03235"
                     /old_locus_tag="pgaptmp_000647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0034228 - ethanolamine transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0034229 - ethanolamine transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization protein EutH"
                     /protein_id="HGU4546643.1"
                     /translation="MGINEIIMYIMMFFMLIAAVDRILSQFGGSARFLGKFGKSIEGS
                     GGQFEEGFMAMGALGLAMVGMTALAPVLAHVLGPVIIPVYEMLGANPSMFAGTLLACD
                     MGGFFLAKELAGGDVAAWLYSGLILGSMMGPTIVFSIPVALGIIEPSDRRYLALGVLA
                     GIVTIPIGCIAGGLVAMYSGVQINGQPVEFTFALILMNMIPVIIVAILVALGLKFIPE
                     KMINGFQIFAKFLVALITLGLAAAVVKFLLGWELIPGLDPIFMAPGDKPGEVMRAIEV
                     IGSISCVLLGAYPMVLLLTRWFEKPLMSVGKVLNMNNIAAAGMVATLANNIPMFGMMK
                     QMDTRGKVINCAFAVSAAFALGDHLGFAAANMNAMIFPMIVGKLIGGVTAIGVAMMLV
                     PKEDATATKTEAEAQS"
     gene            3469..4872
                     /gene="eutA"
                     /locus_tag="ACHOSC_RS03240"
                     /old_locus_tag="pgaptmp_000648"
     CDS             3469..4872
                     /gene="eutA"
                     /locus_tag="ACHOSC_RS03240"
                     /old_locus_tag="pgaptmp_000648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine ammonia-lyase reactivating factor
                     EutA"
                     /protein_id="HGU4546644.1"
                     /translation="MNTRQLLSVGIDIGTTTTQVIFSHLELVNRAAVSQVPRYEFIKR
                     EISWQSPVFFTPVDKQGGLKEAELKTLILEQYQAAGIAPESVDSGAIIITGESAKTRN
                     ARPAVMALSQSLGDFVVASAGPHLESVIAGHGAGAQTLSEQRLCRVLNIDIGGGTANY
                     ALFDAGKISGTACLNVGGRLMETDSQGRVVYAHKPGQMIVDECFGAGTDARSLTGAQL
                     VQVTRRMAALIVEVIDGTLSPLAQALMQTGLLPAGVTPEIITLSGGVGECYRHQPADP
                     FCFADIGPLLATALHDHPRLREMNVQFPAQTVRATVIGAGAHTLSLSGSTIWLEGVQL
                     PLRNLPVAIPIDETDLVSAWQQALLQLDLDPKTDAYVLALPASLPVRYAAVLTVINAL
                     VDFVARFPNPHPLLVVAGQDFGKALGMLLRPQLQQLPLAVIDEVIVRAGDYIDIGTPL
                     FGGSVVPVTVKSLAFPS"
     gene            4884..6245
                     /gene="eutB"
                     /locus_tag="ACHOSC_RS03245"
                     /old_locus_tag="pgaptmp_000649"
     CDS             4884..6245
                     /gene="eutB"
                     /locus_tag="ACHOSC_RS03245"
                     /old_locus_tag="pgaptmp_000649"
                     /EC_number="4.3.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine ammonia-lyase subunit alpha"
                     /protein_id="HGU4546645.1"
                     /translation="MKLKTTLFGNVYQFKDVKEVLAKANELRSGDVLAGVAAASSQER
                     VAAKQVLSEMTVADIRNNPVIAYEDDCVTRLIQDDVNETAYNQIKNWSISELREYVLS
                     DETSVDDIAFTRKGLTSEVVAAVAKICSNADLIYGAKKMPVIKKANTTIGIPGTFSAR
                     LQPNDTRDDVQSIAAQIYEGLSFGVGDAVIGVNPVTDDVENLSRVLDTIYGVIDKFNI
                     PTQGCVLAHVTTQIEAIRRGAPGGLIFQSICGSEKGLKEFGVELAMLDEARAVGAEFN
                     RIAGENCLYFETGQGSALSAGANFGADQVTMEARNYGLARHYDPFIVNTVVGFIGPEY
                     LYNDRQIIRAGLEDHFMGKLSGISMGCDCCYTNHADADQNLNENLMILLATAGCNYIM
                     GMPLGDDIMLNYQTTAFHDTATVRQLLNLRPSPEFERWLESMGIMANGRLTKRAGDPS
                     LFF"
     gene            6266..7153
                     /gene="eutC"
                     /locus_tag="ACHOSC_RS03250"
                     /old_locus_tag="pgaptmp_000650"
     CDS             6266..7153
                     /gene="eutC"
                     /locus_tag="ACHOSC_RS03250"
                     /old_locus_tag="pgaptmp_000650"
                     /EC_number="4.3.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine ammonia-lyase subunit beta"
                     /protein_id="HGU4546646.1"
                     /translation="MDQKQIEEIVRSVMASMGQTAPAPSEAKCATTNCAAPVTSESCA
                     LDLGSAEAKAWIGVENPHRADVLTELRRSTVARVCTGRAGPRPRTQALLRFLADHSRS
                     KDTVLKEVPEEWVKAQGLLEVRSEISDKNLYLTRPDMGRRLCAEAVEALKAQCVANPD
                     VQVVISDGLSTDAITVNYEEILPPLMAGLKQAGLKVGTPFFVRYGRVKIEDQIGEILG
                     AKVVILLVGERPGLGQSESLSCYAVYSPRMATTVEADRTCISNIHQGGTPPVEAAAVI
                     VDLAKRMLEQKASGINMTR"
     gene            7163..7822
                     /gene="eutL"
                     /locus_tag="ACHOSC_RS03255"
                     /old_locus_tag="pgaptmp_000651"
     CDS             7163..7822
                     /gene="eutL"
                     /locus_tag="ACHOSC_RS03255"
                     /old_locus_tag="pgaptmp_000651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031469 - bacterial microcompartment
                     [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization microcompartment
                     protein EutL"
                     /protein_id="HGU4546647.1"
                     /translation="MPALDLIRPSVTAMRVIASVNAEFARELKLPPHIRSLGLISADS
                     DDVTYIAADEATKQAMVEVVYGRSLYAGAAHGPSPTAGEVLIMLGGPNPAEVRAGLDA
                     MVAHIENGAAFQWANDAENTAFLAHVVSRTGSYLSSTAGITLGDPMAYLVAPPLEATY
                     GIDAALKSADVQLVTYVPPPSETNYSAAFLTGSQAACKAACNAFTDAVLEIARNPIQR
                     A"
     gene            7835..8335
                     /gene="eutK"
                     /locus_tag="ACHOSC_RS03260"
                     /old_locus_tag="pgaptmp_000652"
     CDS             7835..8335
                     /gene="eutK"
                     /locus_tag="ACHOSC_RS03260"
                     /old_locus_tag="pgaptmp_000652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416933.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization microcompartment
                     protein EutK"
                     /protein_id="HGU4546648.1"
                     /translation="MINALGLLEVDGMVAAIDAADAMLKAANVRLLSHEVLDPGRLTL
                     VVEGDLAACRAALDAGCAAAMRTGRVISRKEIGRPDDDTQWLVTGFNRQPKQPVREPD
                     APVIVAESADELLALLTSVRQGMTAGEVAAHFGWPLEKARNALEQLFSAGTLRKRSSR
                     YRLKPH"
     gene            8381..9433
                     /gene="eutR"
                     /locus_tag="ACHOSC_RS03265"
                     /old_locus_tag="pgaptmp_000653"
     CDS             8381..9433
                     /gene="eutR"
                     /locus_tag="ACHOSC_RS03265"
                     /old_locus_tag="pgaptmp_000653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator EutR"
                     /protein_id="HGU4546649.1"
                     /translation="MKKTRTANLHHLYHEPLPENLKLTPKVEVDNVHQRQTTDVYEHA
                     LTITAWQQIYDQLHPGKFHCEFTEILLDDIQVFREYTGLALRQSCLVWPNSFWFGIPA
                     TRGEQGFIGSQCLGSAEIATRPGGTEFELSTPDDYTILGVVLSEDVITRQANFLHNPD
                     RVLHMLRSQSALEVKEQHKAALWGFVQQALATFCENPENLHQPAVRKVLGDNLLMAMG
                     AMLEDAQPMVTAESISHQSYRRLLSRAREYVLENMSEPVTVLDLCNQLHVSRRTLQNA
                     FHAILGIGPNAWLKRIRLNAVRRELISPWSQSTTVKDAAMQWGFWHLGQFATDYQQLF
                     AEKPSLTLHQRMREWG"
     gene            complement(9439..10338)
                     /gene="hemF"
                     /locus_tag="ACHOSC_RS03270"
                     /old_locus_tag="pgaptmp_000654"
     CDS             complement(9439..10338)
                     /gene="hemF"
                     /locus_tag="ACHOSC_RS03270"
                     /old_locus_tag="pgaptmp_000654"
                     /EC_number="1.3.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004109 - coproporphyrinogen oxidase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oxygen-dependent coproporphyrinogen oxidase"
                     /protein_id="HGU4546650.1"
                     /translation="MKPDAHQVKQFLLNLQDTICQQLTAVDGAEFVEDSWQREAGGGG
                     RSRVLRNGGVFEQAGVNFSHVHGEAMPASATAHRPELAGRSFEAMGVSLVVHPHNPYV
                     PTSHANVRFFIAEKPGAEPVWWFGGGFDLTPFYGFEEDAIHWHRTARDLCLPFGEDVY
                     PRYKKWCDDYFYLKHRNEQRGIGGLFFDDLNTPDFDHCFAFMQAIGKGYTDAYLPIVE
                     RRKAMAYGERERNFQLYRRGRYVEFNLVWDRGTLFGLQTGGRTESILMSMPPLVRWEY
                     DYQPKDGSPEAALSEFIKVRDWV"
     gene            complement(10342..11211)
                     /gene="amiA"
                     /locus_tag="ACHOSC_RS03275"
                     /old_locus_tag="pgaptmp_000655"
     CDS             complement(10342..11211)
                     /gene="amiA"
                     /locus_tag="ACHOSC_RS03275"
                     /old_locus_tag="pgaptmp_000655"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008745 - N-acetylmuramoyl-L-alanine
                     amidase activity [Evidence IEA];
                     GO_process: GO:0009253 - peptidoglycan catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase AmiA"
                     /protein_id="HGU4546651.1"
                     /translation="MSTFKPLKTLTSRRQVLKAGLAALTLSGMSQAIAKDEPLKTSNG
                     HSKPKAKKSGGKRVVVLDPGHGGIDTGAIGRNGSKEKHVVLAIAKNVRSILRNHGIDA
                     RLTRSGDTFIPLYDRVEIAHKHGADLFMSIHADGFTNPKAAGASVFALSNRGASSAMA
                     KYLSERENRADEVAGKKATDKDHLLQQVLFDLVQTDTIKNSLTLGSHILKKIKPVHKL
                     HSRNTEQAAFVVLKSPSVPSVLVETSFITNPEEERLLGTAAFRQKIATAIAEGVISYF
                     HWFDNQKAHSKKR"
     gene            11425..11850
                     /gene="ypeA"
                     /locus_tag="ACHOSC_RS03280"
                     /old_locus_tag="pgaptmp_000656"
     CDS             11425..11850
                     /gene="ypeA"
                     /locus_tag="ACHOSC_RS03280"
                     /old_locus_tag="pgaptmp_000656"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311332.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family acetyltransferase"
                     /protein_id="HGU4546652.1"
                     /translation="MEIRVFRQEDFEEVITLWERCDLLRPWNDPEMDIERKMNHDVSL
                     FLVAEVNGEVVGTVMGGYDGHRGSAYYLGVHPEFRGRGIANALLNRLEKKLIARGCPK
                     IQINVPEDNDMVLGMYERLGYEHADVLSLGKRLIEDEEY"
     gene            11837..12286
                     /gene="yfeZ"
                     /locus_tag="ACHOSC_RS03285"
                     /old_locus_tag="pgaptmp_000657"
     CDS             11837..12286
                     /gene="yfeZ"
                     /locus_tag="ACHOSC_RS03285"
                     /old_locus_tag="pgaptmp_000657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708284.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2919 domain-containing protein"
                     /protein_id="HGU4546653.1"
                     /translation="MKSTEFHPVHYDAHGRLRLPLLFWLVLLLQARTWVLFVIAGASR
                     EQGTALLNLFYPDHDNFWLGLIPGIPAVLAFLLSGRRATFPRTWHVLYFLLLLAQVVL
                     LCWQPWLWLNGESVSGIGLALVVADIVALIWLLTNRRLRACFNEEKE"
     gene            12347..12922
                     /gene="yfeY"
                     /locus_tag="ACHOSC_RS03290"
                     /old_locus_tag="pgaptmp_000658"
     CDS             12347..12922
                     /gene="yfeY"
                     /locus_tag="ACHOSC_RS03290"
                     /old_locus_tag="pgaptmp_000658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RpoE-regulated lipoprotein"
                     /protein_id="HGU4546654.1"
                     /translation="MKSLRLMLCAMPLMLTGCSTMSSVNWSAANPWNWFGSSTKVSEQ
                     GVGELTASTPLQEQAIADALDGDYRLRSGMKTANGNVVRFFEVMKGDNVAMVINGDQG
                     TISRIDVLDSDIPADTGVKIGTPFSDLYSKAFGNCQKADGDDNRAVECKAEGSQHISY
                     QFSGEWSGPEGLMPSDDTLKNWKVSKIIWRR"
     gene            13018..13917
                     /gene="yfeX"
                     /locus_tag="ACHOSC_RS03295"
                     /old_locus_tag="pgaptmp_000659"
     CDS             13018..13917
                     /gene="yfeX"
                     /locus_tag="ACHOSC_RS03295"
                     /old_locus_tag="pgaptmp_000659"
                     /EC_number="4.98.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311329.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porphyrinogen peroxidase"
                     /protein_id="HGU4546655.1"
                     /translation="MSQVQSGILPEHCRAAIWIEANVKGEVDALRAASKTFADKLATF
                     EAKFPDAHLGAVVAFGNNTWRALSGGVGAEELKDFPGYGKGLAPTTQFDVLIHILSLR
                     HDVNFSVAQAAMEAFGDCIEVKEEIHGFRWVEERDLSGFVDGTENPAGEETRREVAVI
                     KDGVDAGGSYVFVQRWEHNLKQLNRMSVHDQEMMIGRTKEANEEIDGDERPETSHLTR
                     VDLKEDGKGLKIVRQSLPYGTASGTHGLYFCAYCARLHNIEQQLLSMFGDTDGKRDAM
                     LRFTKPVTGGYYFAPSLDKLMAL"
     gene            complement(13975..15279)
                     /gene="pbp4b"
                     /locus_tag="ACHOSC_RS03300"
                     /old_locus_tag="pgaptmp_000660"
     CDS             complement(13975..15279)
                     /gene="pbp4b"
                     /locus_tag="ACHOSC_RS03300"
                     /old_locus_tag="pgaptmp_000660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin binding protein PBP4B"
                     /protein_id="HGU4546656.1"
                     /translation="MKRTMLYLSLLAVSCSVSAAKYPVLTESSPEKAGFNVERLNQMD
                     RWISQQVDAGYPGVNLLIIKDNQIVYRKAWGAAKKYDGSVLMEQPVKATTGTLYDLAS
                     NTKMYATNFALQKLMSEGKLHPDDRIAKYIPGFADSPNDTIKGKNTLRISDLLHHSGG
                     FPADPQYPNKAVAGALYSQDKGQTLEMIKRTPLEYQPGSKHIYSDVDYMLLGFIVESV
                     TGQPLDRYVEESIYRPLGLTHTVFNPLLKGFKPQQIAATELNGNTRDGVIHFPNIRTS
                     TLWGQVHDEKAFYSMGGVSGHAGLFSNTGDIAVLMQTMLNGGGYGDVQLFSAETVKMF
                     TTSSKEDATFGLGWRVNGNATMTPTFGTLASPQTYGHTGWTGTVTVIDPVNHMAIVML
                     SNKPHSPVADPQKNPNMFESGQLPIATYGWVVDQVYAALKQK"
     gene            complement(15284..16708)
                     /gene="murP"
                     /locus_tag="ACHOSC_RS03305"
                     /old_locus_tag="pgaptmp_000661"
     CDS             complement(15284..16708)
                     /gene="murP"
                     /locus_tag="ACHOSC_RS03305"
                     /old_locus_tag="pgaptmp_000661"
                     /EC_number="2.7.1.192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS N-acetylmuramic acid transporter subunit
                     IIBC"
                     /protein_id="HGU4546657.1"
                     /translation="MAKEISSELLNTILTRVGGPGNIASCGNCMTRLRLGVHDSSLVD
                     PNIKTLEGVKGVILTSDQVQVVFGPGKAHRAAKAMSELLGEAPVQDAAEIAAQNKRQL
                     KAKQTSGVQQFLAKFATIFTPLIPGFIAAGLLLGIATLIATVMHVPADAQGTLPDALN
                     FMKVFSKGLFTFLVILVGYNAAQAFGGTGVNGAIIAALFLLGYNPAATTGYYAGFHDF
                     FGLPIDPRGNIIGVLIAAWACARIEGMVRRFMPDDLDMLLTSLITLLITATLAYLIIM
                     PLGGWLFEGMSWLFMHLNSNPFGCAVLAGLFLIAVVFGVHQGFIPVYLALMDSQGFNS
                     LFPILSMAGAGQVGAALALYWRAQPHSALRSQVRGAIIPGLLGVGEPLIYGVTLPRMK
                     PFITACLGGAAGGLFIGLIAWWGLPMGLNSAFGPSGLVALPLMTSAQGILPAMAVYAG
                     GILVAWVCGFIFTTLFGCRNVNLD"
     gene            complement(16712..17608)
                     /gene="murQ"
                     /locus_tag="ACHOSC_RS03310"
                     /old_locus_tag="pgaptmp_000662"
     CDS             complement(16712..17608)
                     /gene="murQ"
                     /locus_tag="ACHOSC_RS03310"
                     /old_locus_tag="pgaptmp_000662"
                     /EC_number="4.2.1.126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016835 - carbon-oxygen lyase activity
                     [Evidence IEA];
                     GO_process: GO:0009254 - peptidoglycan turnover [Evidence
                     IEA];
                     GO_process: GO:0046348 - amino sugar catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramic acid 6-phosphate etherase"
                     /protein_id="HGU4546658.1"
                     /translation="MQLEKMITEGSNTASAEIDRVSTLEMCRIINDEDKTVPLAVERV
                     LPDIAAAIDVIHAQVSGGGRLIYLGAGTSGRLGILDASECPPTYGVKPGLVVGLIAGG
                     EYAIQHAVEGAEDSREGGVNDLKNINLTAQDVVVGIAASGRTPYVIAGLEYARQLGCR
                     TVGISCNPGSAVSTTAEFAITPIVGAEVVTGSSRMKAGTAQKLVLNMLSTGLMIKSGK
                     VFGNLMVDVVATNEKLHVRQVNIVKNATGCNAEQAEAALIACERNCKTAIVMVLKNLD
                     AAEAKKRLDQHGGFIRQVLDKE"
     gene            17772..18629
                     /gene="murR"
                     /locus_tag="ACHOSC_RS03315"
                     /old_locus_tag="pgaptmp_000663"
     CDS             17772..18629
                     /gene="murR"
                     /locus_tag="ACHOSC_RS03315"
                     /old_locus_tag="pgaptmp_000663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator MurR"
                     /protein_id="HGU4546659.1"
                     /translation="MLYLTKISNAGSEFTENEQKIADFLQANVSELQSVSSRKMAKQL
                     GISQSSIVKFAQKLGAQGFTELRMALIGEYSASREKTNATALHLHSSITSDDSLEVIA
                     RKLNREKELALEQTCALFDYARLQKIIDVISKAQFIQITGLGGSALVGRDLSFKLMKI
                     GYRVACEADTHVQATVSQALKKGDVQIAISYSGSKKEIVLCAEAARKQGATVIAITSL
                     ADSPLRRLAHFTLDTVSGETEWRSSSMSTRTAQNSVTDLLFVGLVQLNDVESLKMIQR
                     SSELTQRLK"
     gene            18758..19549
                     /gene="ucpA"
                     /locus_tag="ACHOSC_RS03320"
                     /old_locus_tag="pgaptmp_000664"
     CDS             18758..19549
                     /gene="ucpA"
                     /locus_tag="ACHOSC_RS03320"
                     /old_locus_tag="pgaptmp_000664"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311324.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase UcpA"
                     /protein_id="HGU4546660.1"
                     /translation="MGKLTGKTALITGALQGIGEGIARTFARHGANLILLDISPEIEK
                     LADELCGRGHRCTAVVADVRDPASVAAAIKRAKEKEGRIDILVNNAGVCRLGSFLDMS
                     DEDRDFHIDINIKGVWNVTKAVLPEMIARKDGRIVMMSSVTGDMVADPGETAYALTKA
                     AIVGLTKSLAVEYAQSGIRVNAICPGYVRTPMAESIARQSNPEDPESVLTDMAKAIPL
                     RRLADPLEVGELAAFLASDESSYLTGTQNVIDGGSTLPETVSVGI"
     gene            19720..20736
                     /gene="cysP"
                     /locus_tag="ACHOSC_RS03325"
                     /old_locus_tag="pgaptmp_000665"
     CDS             19720..20736
                     /gene="cysP"
                     /locus_tag="ACHOSC_RS03325"
                     /old_locus_tag="pgaptmp_000665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiosulfate/sulfate ABC transporter
                     substrate-binding protein CysP"
                     /protein_id="HGU4546661.1"
                     /translation="MAVNLLKKNSLALVASLLLAGHVQATELLNSSYDVSRELFAALN
                     PPFEQQWAKDNGGDKLTIKQSHAGSSKQALAILQGLKADVVTYNQVTDVQILHDKGKL
                     IPADWQSRLPNNSSPFYSTMGFLVRKGNPKNIHDWNDLVRSDVKLIFPNPKTSGNARY
                     TYLAAWGAADKADGGDKAKTEQFMTQFLKNVEVFDTGGRGATTTFAERGLGDVLISFE
                     SEVNNIRKQYEAQGFEVVIPKTNILAEFPVAWVDKNVQANGTEKAAKAYLNWLYSPQA
                     QTIITDYYYRVNNPEVMDKLKDKFPQTELFRVEDKFGSWPEVMKTHFTSGGELDKLLA
                     AGRN"
     gene            20736..21569
                     /gene="cysT"
                     /locus_tag="ACHOSC_RS03330"
                     /old_locus_tag="pgaptmp_000666"
     CDS             20736..21569
                     /gene="cysT"
                     /locus_tag="ACHOSC_RS03330"
                     /old_locus_tag="pgaptmp_000666"
                     /EC_number="7.3.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000458418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015419 - ABC-type sulfate transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008272 - sulfate transport [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate/thiosulfate ABC transporter permease
                     CysT"
                     /protein_id="HGU4546662.1"
                     /translation="MFAVSSRRVLPGFTLSLGTSLLFVCLILLLPLSALVMQLAQMSW
                     AQYWEVITNPQVVAAYKVTLLSAFVASIFNGVFGLLMAWILTRYRFPGRTLLDALMDL
                     PFALPTAVAGLTLASLFSVNGFYGEWLAKFDIKVTYTWLGIAVAMAFTSIPFVVRTVQ
                     PVLEELGPEYEEAAETLGATRWQSFCKVVLPELSPALVAGVALSFTRSLGEFGAVIFI
                     AGNIAWKTEVTSLMIFVRLQEFDYPAASAIASVILAASLLLLFSINTLQSRFGRRVVG
                     H"
     gene            21569..22444
                     /gene="cysW"
                     /locus_tag="ACHOSC_RS03335"
                     /old_locus_tag="pgaptmp_000667"
     CDS             21569..22444
                     /gene="cysW"
                     /locus_tag="ACHOSC_RS03335"
                     /old_locus_tag="pgaptmp_000667"
                     /EC_number="7.3.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026168.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015419 - ABC-type sulfate transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008272 - sulfate transport [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate/thiosulfate ABC transporter permease
                     CysW"
                     /protein_id="HGU4546663.1"
                     /translation="MAEVTQLKRYDARPINWGKWFLIGIGMLVSAFILLVPMIYIFVQ
                     AFSKGLMPVLQNLADPDMLHAIWLTVMIALIAVPVNLVFGILLAWLVTRFNFPGRQLL
                     LTLLDIPFAVSPVVAGLVYLLFYGSNGPLGGWLDEHNLQIMFSWPGMVLVTIFVTCPF
                     VVRELVPVMLSQGSQEDEAAILLGASGWQMFRRVTLPNIRWALLYGVVLTNARAIGEF
                     GAVSVVSGSIRGETLSLPLQIELLEQDYNTVGSFTAAALLTLMAIITLFLKSMLQWRL
                     ENQDKRAQQEEHHEH"
     gene            22434..23531
                     /gene="cysA"
                     /locus_tag="ACHOSC_RS03340"
                     /old_locus_tag="pgaptmp_000668"
     CDS             22434..23531
                     /gene="cysA"
                     /locus_tag="ACHOSC_RS03340"
                     /old_locus_tag="pgaptmp_000668"
                     /EC_number="7.3.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015419 - ABC-type sulfate transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008272 - sulfate transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate/thiosulfate ABC transporter ATP-binding
                     protein CysA"
                     /protein_id="HGU4546664.1"
                     /translation="MSIEIANIKKSFGRTQVLNDISLDIPSGQMVALLGPSGSGKTTL
                     LRIIAGLEHQTSGHIRFHGTDVSRLHARDRKVGFVFQHYALFRHMTVFDNIAFGLTVL
                     PRRERPNAAAIKAKVTKLLEMVQLAHLADRYPAQLSGGQKQRVALARALAVEPQILLL
                     DEPFGALDAQVRKELRRWLRQLHEELKFTSVFVTHDQEEATEVADRVVVMSQGNIEQA
                     DAPNQVWREPATRFVLEFMGEVNRLQGTIRGGQFHVGAHRWPLGYTPAYQGPVDLFLR
                     PWEVDISRRTSLDSPLPVQVLEASPKGHYTQLVVQPLGWYNEPLTVVMHGDDAPQRGE
                     RLFVGLQHARLYNGDERIETRDEELALAQSA"
     gene            23665..24576
                     /gene="cysM"
                     /locus_tag="ACHOSC_RS03345"
                     /old_locus_tag="pgaptmp_000669"
     CDS             23665..24576
                     /gene="cysM"
                     /locus_tag="ACHOSC_RS03345"
                     /old_locus_tag="pgaptmp_000669"
                     /EC_number="2.5.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004124 - cysteine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006535 - cysteine biosynthetic process
                     from serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase B"
                     /protein_id="HGU4546665.1"
                     /translation="MSTLEQTIGNTPLVKLQRMGPDNGSEVWLKLEGNNPAGSVKDRA
                     ALSMIVEAEKRGEIKPGDVLIEATSGNTGIALAMIAALKGYRMKLLMPDNMSQERRAA
                     MRAYGAELILVTKEQGMEGARDLALEMANRGEGKLLDQFNNPDNPYAHYTTTGPEIWQ
                     QTGGRITHFVSSMGTTGTITGVSRFMREQSKPVTIVGLQPEEGSSIPGIRRWPAEYLP
                     GIFNASLVDEVLDIHQRDAENTMRELAVREGIFCGVSSGGAVAGALRVAKANPGAVVV
                     AIICDRGDRYLSTGVFGEEHFSQGAGI"
     gene            complement(24579..24947)
                     /gene="yfeK"
                     /locus_tag="ACHOSC_RS03350"
                     /old_locus_tag="pgaptmp_000670"
     CDS             complement(24579..24947)
                     /gene="yfeK"
                     /locus_tag="ACHOSC_RS03350"
                     /old_locus_tag="pgaptmp_000670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001592994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfeK family protein"
                     /protein_id="HGU4546666.1"
                     /translation="MKKIICLVITLLMTLPAYAKLTAHEEARINAMLEGLAQKKDLIF
                     VRNGDEHTCDEAVSHLRLKLGNTRNRIDTAEQFIDKVASSSSITGKPYIVKIPGKSDE
                     NAQPFLHALIAQTDKTVPAQ"
     gene            25052..25903
                     /gene="pdxK"
                     /locus_tag="ACHOSC_RS03355"
                     /old_locus_tag="pgaptmp_000671"
                     /gene_synonym="thiJ"
     CDS             25052..25903
                     /gene="pdxK"
                     /locus_tag="ACHOSC_RS03355"
                     /old_locus_tag="pgaptmp_000671"
                     /gene_synonym="thiJ"
                     /EC_number="2.7.1.35"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008478 - pyridoxal kinase activity
                     [Evidence IEA];
                     GO_process: GO:0009443 - pyridoxal 5'-phosphate salvage
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxine/pyridoxal/pyridoxamine kinase"
                     /protein_id="HGU4546667.1"
                     /translation="MSSLLLFNDKSRALQADIVAVQSQVVYGSVGNSIAVPAIKQNGL
                     NVFAVPTVLLSNTPHYDTFYGGAIPDEWFSGYLRALQERDALRQLRAVTTGYMGTASQ
                     IKILAEWLTALRKDHPDLLIMVDPVIGDIDSGIYVKPDLPEAYRQYLLPLAQGITPNI
                     FELEILTGKNCRDLDSAIAAAKSLLSDTLKWVVITSASGNEENQEMQVVVVSADSVNV
                     ISHSRVKTDLKGTGDLFCAQLISGLLKGKALNDAVHRAGLRVLEVMRYTQQHESDELI
                     LPPLAEA"
     gene            complement(25945..26454)
                     /gene="crr"
                     /locus_tag="ACHOSC_RS03360"
                     /old_locus_tag="pgaptmp_000672"
     CDS             complement(25945..26454)
                     /gene="crr"
                     /locus_tag="ACHOSC_RS03360"
                     /old_locus_tag="pgaptmp_000672"
                     /EC_number="2.7.1.199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015833547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HGU4546668.1"
                     /translation="MGLFDKLKSLVSDDKKDTGTIEIIAPLSGEIVNIEDVPDVVFAE
                     KIVGDGIAIKPTGNKMVAPVDGTIGKIFETNHAFSIESDSGVELFVHFGIDTVELKGE
                     GFKRIAEEGQRVKVGDTVIEFDLPLLEEKAKSTLTPVVISNMDEIKELIKLSGSVTVG
                     ETPVIRIKK"
     gene            complement(26495..28222)
                     /gene="ptsI"
                     /locus_tag="ACHOSC_RS03365"
                     /old_locus_tag="pgaptmp_000673"
     CDS             complement(26495..28222)
                     /gene="ptsI"
                     /locus_tag="ACHOSC_RS03365"
                     /old_locus_tag="pgaptmp_000673"
                     /EC_number="2.7.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate-protein phosphotransferase
                     PtsI"
                     /protein_id="HGU4546669.1"
                     /translation="MISGILASPGIAFGKALLLKEDEIVIDRKKISADQVDQEVERFL
                     SGRAKASAQLETIKTKAGETFGEEKEAIFEGHIMLLEDEELEQEIIALIKDKHMTADA
                     AAHEVIEGQASALEELDDEYLKERAADVRDIGKRLLRNILGLKIIDLSAIQDEVILVA
                     ADLTPSETAQLNLKKVLGFITDAGGRTSHTSIMARSLELPAIVGTGSVTSQVKNDDYL
                     ILDAVNNQVYVNPTNEVIDKMRAVQEQVASEKAELAKLKDLPAITLDGHQVEVCANIG
                     TVRDVEGAERNGAEGVGLYRTEFLFMDRDALPTEEEQFAAYKAVAEACGSQAVIVRTM
                     DIGGDKELPYMNFPKEENPFLGWRAIRIAMDRKEILRDQLRAILRASAFGKLRIMFPM
                     IISVEEVRALRKEIEIYKQELRDEGKAFDESIEIGVMVETPAAATIARHLAKEVDFFS
                     IGTNDLTQYTLAVDRGNDMISHLYQPMSPSVLNLIKQVIDASHAEGKWTGMCGELAGD
                     ERATLLLLGMGLDEFSMSAISIPRIKKIIRNTNFEDAKVLAEQALAQPTTDELMTLVN
                     KFIEEKTIC"
     gene            complement(28267..28524)
                     /gene="ptsH"
                     /locus_tag="ACHOSC_RS03370"
                     /old_locus_tag="pgaptmp_000674"
     CDS             complement(28267..28524)
                     /gene="ptsH"
                     /locus_tag="ACHOSC_RS03370"
                     /old_locus_tag="pgaptmp_000674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003038086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphocarrier protein Hpr"
                     /protein_id="HGU4546670.1"
                     /translation="MFQQEVTITAPNGLHTRPAAQFVKEAKGFTSEITVTSNGKSASA
                     KSLFKLQTLGLTQGTVVTISAEGEDEQKAVEHLVKLMAELE"
     gene            complement(28908..29879)
                     /gene="cysK"
                     /locus_tag="ACHOSC_RS03375"
                     /old_locus_tag="pgaptmp_000675"
     CDS             complement(28908..29879)
                     /gene="cysK"
                     /locus_tag="ACHOSC_RS03375"
                     /old_locus_tag="pgaptmp_000675"
                     /EC_number="2.5.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000034402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004124 - cysteine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006535 - cysteine biosynthetic process
                     from serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase A"
                     /protein_id="HGU4546671.1"
                     /translation="MSKIFEDNSLTIGHTPLVRLNRIGNGRILAKVESRNPSFSVKCR
                     IGANMIWDAEKRGVLKPGVELVEPTSGNTGIALAYVAAARGYKLTLTMPETMSIERRK
                     LLKALGANLVLTEGAKGMKGAIQKAEEIVASNPEKYLLLQQFSNPANPEIHEKTTGPE
                     IWEDTDGQVDVFIAGVGTGGTLTGVSRYIKGTKGKTDLISVAVEPTDSPVIAQALAGE
                     EIKPGPHKIQGIGAGFIPANLDLKLVDKVIGITNEEAISTARRLMEEEGILAGISSGA
                     AVAAALKLQEDESFTNKNIVVILPSSGERYLSTALFADLFTEKELQQ"
     gene            complement(30064..30825)
                     /gene="cysZ"
                     /locus_tag="ACHOSC_RS03380"
                     /old_locus_tag="pgaptmp_000676"
     CDS             complement(30064..30825)
                     /gene="cysZ"
                     /locus_tag="ACHOSC_RS03380"
                     /old_locus_tag="pgaptmp_000676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015116 - sulfate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0000103 - sulfate assimilation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate transporter CysZ"
                     /protein_id="HGU4546672.1"
                     /translation="MVSSFTSAPRSGFYYFAQGWKLVSQPGIRRFVILPLLVNILLMG
                     GAFWWLFTQLDVWIPTLMSYVPDWLQWLSYLLWPLAVISVLLVFGYFFSTIANWIAAP
                     FNGLLAEQLEARLTGATPPDTGIFGIMKDVPRIMKREWQKFAWYLPRAIVLLILYLIP
                     GIGQTVAPVLWFLFSAWMLAIQYCDYPFDNHKVPFKEMRTALRTRKITNMQFGALTSL
                     FTMIPLLNLFIMPVAVCGATAMWVDCYRDKHAMWR"
     gene            31055..32041
                     /gene="zipA"
                     /locus_tag="ACHOSC_RS03385"
                     /old_locus_tag="pgaptmp_000677"
     CDS             31055..32041
                     /gene="zipA"
                     /locus_tag="ACHOSC_RS03385"
                     /old_locus_tag="pgaptmp_000677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0000917 - division septum assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZipA"
                     /protein_id="HGU4546673.1"
                     /translation="MMQDLRLILIIVGAIAIIALLVHGFWTSRKERSSMFRDRPLKRM
                     KSKRDDDSYDEDVEDDEGVGEVRVHRVNHAPANAQEHEAARPSPQHQYQPPYASAQPR
                     QPVQQPPEAQVPPQHAPRPAQPVQQPAYQPQPEQPLQQPVSPQVAPAPQPVHSAPQPA
                     QQAFQPAEPVAAPQPEPVAEPAPVMDKPKRKEAVIIMNVAAHHGSELNGELLLNSIQQ
                     AGFIFGDMNIYHRHLSPDGSGPALFSLANMVKPGTFDPEMKDFTTPGVTIFMQVPSYG
                     DELQNFKLMLQSAQHIADEVGGVVLDDQRRMMTPQKLREYQDIIREVKDANA"
     gene            32112..34127
                     /gene="ligA"
                     /locus_tag="ACHOSC_RS03390"
                     /old_locus_tag="pgaptmp_000678"
     CDS             32112..34127
                     /gene="ligA"
                     /locus_tag="ACHOSC_RS03390"
                     /old_locus_tag="pgaptmp_000678"
                     /EC_number="6.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003911 - DNA ligase (NAD+) activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent DNA ligase LigA"
                     /protein_id="HGU4546674.1"
                     /translation="MESIEQQLTELRTTLRHHEYLYHVMDAPEIPDAEYDRLMRELRE
                     LETKHPELITPDSPTQRVGAAPLAAFSQIRHEVPMLSLDNVFDEESFLAFNKRVQDRL
                     KNNEKVTWCCELKLDGLAVSILYENGVLVSAATRGDGTTGEDITSNVRTIRAIPLKLH
                     GENIPARLEVRGEVFLPQAGFEKINEDARRTGGKVFANPRNAAAGSLRQLDPRITAKR
                     PLTFFCYGVGVLEGGELPDTHLGRLLQFKKWGLPVSDRVTLCESAEEVLAFYHKVEED
                     RPTLGFDIDGVVIKVNSLEQQEQLGFVARAPRWAVAFKFPAQEQMTFVRDVEFQVGRT
                     GAITPVARLEPVHVAGVLVSNATLHNADEIERLGLRIGDKVVIRRAGDVIPQVVNVVL
                     SERPEDTREVVFPTHCPVCGSDVERVEGEAVARCTGGLICGAQRKESLKHFVSRRAMD
                     VDGMGDKIIDQLVEKEYVHTPADLFKLTAGKLTGLERMGPKSAQNVVNALEKAKETTF
                     ARFLYALGIREVGEATAAGLAAYFGTLEALEAASIEELQKVPDVGIVVASHVHNFFAE
                     ESNRNVISELLAEGVHWPAPIVINAEEIDSPFAGKTVVLTGSLSQMSRDDAKARLVEL
                     GAKVAGSVSKKTDLVIAGEAAGSKLAKAQELGIEVIDEAEMLRLLGS"
     gene            34129..34347
                     /gene="ypeB"
                     /locus_tag="ACHOSC_RS03395"
                     /old_locus_tag="pgaptmp_000679"
     CDS             34129..34347
                     /gene="ypeB"
                     /locus_tag="ACHOSC_RS03395"
                     /old_locus_tag="pgaptmp_000679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3820 family protein"
                     /protein_id="HGU4546675.1"
                     /translation="MEKEQLIEIANTIMPFGKYKGRRLIDLPEEYLLWFARKDEFPAG
                     KLGELMQITLLIKTEGLTQLVQPLKRPL"
     gene            complement(34344..35342)
                     /gene="yfeH"
                     /locus_tag="ACHOSC_RS03400"
                     /old_locus_tag="pgaptmp_000680"
     CDS             complement(34344..35342)
                     /gene="yfeH"
                     /locus_tag="ACHOSC_RS03400"
                     /old_locus_tag="pgaptmp_000680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015293 - symporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bile acid:sodium symporter family protein"
                     /protein_id="HGU4546676.1"
                     /translation="MKLFRILDPFTLTLITVVLLASFFPARGDFVPFFENLTTAAIAL
                     LFFMHGAKLSREAIIAGGGHWRLHLWVICSTFVLFPILGVLFAWWKPVNVDPMLYSGF
                     LYLCILPATVQSAIAFTSMAGGNVAAAVCSASASSLLGIFLSPLLVGLVMNVHGAGGS
                     LEQVGKIMLQLLLPFVLGHLSRPWIGDWVSRNKKWIAKTDQTSILLVVYTAFSEAVVN
                     GIWHKVGWGSLLFIVVVSCVLLAIVIVVNVFMARRLGFNKADEITIVFCGSKKSLANG
                     IPMANILFPTSVIGMMVLPLMIFHQIQLMVCAVLARRYKRQTEQLQAQQESSADKA"
     gene            35432..36358
                     /gene="yfeR"
                     /locus_tag="ACHOSC_RS03405"
                     /old_locus_tag="pgaptmp_000681"
     CDS             35432..36358
                     /gene="yfeR"
                     /locus_tag="ACHOSC_RS03405"
                     /old_locus_tag="pgaptmp_000681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HGU4546677.1"
                     /translation="MNYSLKQLKVFVTVAQEKSFSRAGERIGLSQSAVSHSVKELENH
                     TGVRLLDRTTREVVLTDAGQQLALRLERLLDELNSTLRDTGRMGQQLSGKVRVAASQT
                     ISAHLIPQCIAESHRRYPDIQFVLHDRPQQWVMESIRQGDVDFGIVIDPGPVGDLQCE
                     AILSEPFFLLCHRDSALAVEDYVPWQALQGAKLVLQDYASGSRPLIDAALARNGIQAN
                     IVQEIGHPATLFPMVAAGIGISILPALALPLPEGSPLVVKRITPVVERQLMLVRRKNR
                     SLSTAAEALWDVVRDQGNALMAGREGDPLYQI"
     gene            complement(36397..37161)
                     /gene="yfeN"
                     /locus_tag="ACHOSC_RS03410"
                     /old_locus_tag="pgaptmp_000682"
     CDS             complement(36397..37161)
                     /gene="yfeN"
                     /locus_tag="ACHOSC_RS03410"
                     /old_locus_tag="pgaptmp_000682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein OmpK"
                     /protein_id="HGU4546678.1"
                     /translation="MKKHLLTLTLSSILAIPVVSHAEFKGGFADIGVHYLDWTSRTTE
                     KSSTKSHKDDFGYLEFEGGANFSWGEMYGFFDWENFYNGRHNKPGSEQRYTFKNTNRI
                     YLGDTGFNLYLHAYGTYGSANRVNFHDDMFLYGIGYNFTGSGWWFKPFFAKRYTDQTY
                     YTGDNGYVAGWVAGYNFMLGSEKFTLTNWNEYEFDRDATYAAGNGGKEGLNGAVALWW
                     NATSHITTGIQYRYADDKLGEDFYQDAIIYSIKFNF"
     gene            37410..38243
                     /gene="xapA"
                     /locus_tag="ACHOSC_RS03415"
                     /old_locus_tag="pgaptmp_000683"
     CDS             37410..38243
                     /gene="xapA"
                     /locus_tag="ACHOSC_RS03415"
                     /old_locus_tag="pgaptmp_000683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004731 - purine-nucleoside phosphorylase
                     activity [Evidence IEA];
                     GO_process: GO:0055086 - nucleobase-containing small
                     molecule metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="xanthosine phosphorylase"
                     /protein_id="HGU4546679.1"
                     /translation="MSQVQFSHNPLFCIDIIKTYKPDFTPRVAFILGSGLGALADQIE
                     NAVAISYEKLPGFPVSTVHGHAGELVLGHLQGVPVVCMKGRGHFYEGRGMTIMTDAIR
                     TFKLLGCELLFCTNAAGSLRPEVGAGSLVALKDHINTMPGTPMVGLNDDRFGERFFSL
                     ANAYDAEYRALLQKVAKEEGFPLTEGVFVSYPGPNFETAAEIRMMQIIGGDVVGMSVV
                     PEVISARHCDLKVVAVSAITNMAEGLSDVKLSHAQTLAAAELSKQNFINLICGFLRKI
                     A"
     gene            38303..39559
                     /gene="xapB"
                     /locus_tag="ACHOSC_RS03420"
                     /old_locus_tag="pgaptmp_000684"
     CDS             38303..39559
                     /gene="xapB"
                     /locus_tag="ACHOSC_RS03420"
                     /old_locus_tag="pgaptmp_000684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthosine/proton symporter XapB"
                     /protein_id="HGU4546680.1"
                     /translation="MSIAMRLKVMSFLQYFIWGSWLVTLGSYMINTLHFTGANVGMVY
                     SSKGIAAIIMPGIMGIIADKWLRAERAYMLCHLVCAGVLFYAASVTDPDMMFWVMLVN
                     AMAFMPTIALSNSVSYSCLAQAGLDPVTAFPPIRVFGTVGFIVAMWAVSLLHLELSSL
                     QLYIASGASLLLSAYALTLPKIPVAEKKATTSLASKLGLDAFVLFKNPRMAIFFLFAM
                     MLGAVLQITNVFGNPFLHDFARNPEFADSFVVKYPSILLSVSQMAEVGFILTIPFFLK
                     RFGIKTVMLMSMVAWTLRFGFFAYGDPSTTGFILLLLSMIVYGCAFDFFNISGSVFVE
                     QEVDSSIRASAQGLFMTMVNGVGAWVGSILSGMAVDYFSVDGVKDWQTIWLVFAGYAL
                     FLAVIFFFGFKYNHDPEKIKHRAVAH"
BASE COUNT         9238 a        10019 c        10997 g         9408 t
ORIGIN      
        1 accaccatga ccatcaccac gccaaccggt gaaggggtaa ccagcgcgcg gacgtttgtc
       61 cgtctgcgtc gctgtgtatt agtcgatgcg tttcgcattg tttaaggagt aaccgatggc
      121 gcacgacgaa caatggctca ccccacgtct gcaaacggcg gcaacgctgt gtaaccagac
      181 gcctgccgcg acggaatctc cgctgtggct gggggtggat ctgggcacct gcgatgtggt
      241 gtcgatggtt gttgaccgcg acggtcagcc ggtggcggta tgcctcgact gggccgatgt
      301 ggtccgcgac ggcatcgtct gggatttctt cggtgcggtc accattgttc gtcgccatct
      361 cgacactctt gagcagcaat tcggtcttcg ttttagccat gcggcgactt catttcctcc
      421 aggtacggac ccgcgtattt ccatcaacgt gctggaatct gccgggctgg aagtgagcca
      481 tgtgctggat gagccaaccg cagtcgcgga tctgctgcaa ctggacaacg ccggtgtagt
      541 ggatatcggc ggcggcacta ccggcattgc catcgtgaaa aagggcaagg tgacgtactc
      601 ggcggatgaa gccaccggcg gacatcacat ctctctgact ctcgccggaa atcgtcgtat
      661 ttcgctggaa gaggcagagc agtacaagcg cggtcatggt gacgagattt ggcccgcggt
      721 gaaaccggtg tacgaaaaaa tggcggacat tgtcgcccgg catattgaag ggcagggcat
      781 tactgattta tggctggcgg gcggctcttg tatgcaaccg ggcgtggcgg aactgtttcg
      841 caaacaattc ccggcgttac aggtgcattt accgcagcac agtttgttta tgaccccgct
      901 ggcgatcgcc agtagtgggc gagagaaagc ggaggggcta tatgcaaaat gaattgcaga
      961 ccgcgctctt tcaggcgttc gataccctga atctgcaacg ggtaaaaaca tttagcgttc
     1021 caccggtgac gctttgcggt ccgggctcgg tgagcagttg cggacagcaa gcgcaaacgc
     1081 gtgggctgaa acatctgttc gtgatggcag acagcttttt gcatcaggca gggatgaccg
     1141 ccgggctgac gcgtagcctg gccgttaaag gtatcgccat gacgctctgg ccatgtccgg
     1201 tgggcgaacc gtgcatcacc gacgtgtgtg cagccgtggc gcagttgcgt gagtcaggct
     1261 gtgatggggt gatcgcattt ggcggcggct cggtgctgga tgcggcgaaa gccgtggcgt
     1321 tgctggtgac gaacccggat agcacgctgg cagagatgtc agaaaccagc gttctgcaat
     1381 cgcgcctgcc gttgattgcc attcccacta ccgccggaac cggctctgaa accaccaacg
     1441 taacggtgat tatcgatgcg gtgagcgggc gtaagcaggt gttagcccat gcctcgctga
     1501 tgccggatgt ggcgattctc gacgccgcat tgaccgaagg tgtgccgtcg catgtcacgg
     1561 cgatgaccgg cattgatgcg ttaacccatg ccattgaagc atacagcgcc ctgaacgcta
     1621 caccgtttac cgacagtctg gcgattggtg ccattgcgat gattggcaaa tcgctgccga
     1681 aagcggtggg ctacggtcat gaccttgccg cgcgcgagag catgttactg gcttcatgta
     1741 tggcgggaat ggcgttttcc agtgcggggc ttgggttgtg ccacgcgatg gcgcatcagc
     1801 caggggcggc gctgcatatt ccacacggtc tggcgaacgc catgttgctg ccaacggtga
     1861 tggaatttaa ccggatggtt tgtcgcgaac ggtttagtca gattggtcgg gcactgcgaa
     1921 ctaaaaaatc cgacgatcgt gacgctatta acgcggtaag tgagctgatt gcggaagttg
     1981 ggattggtaa acgactgggc gatgttggcg cgacatctgc gcattacggt gcatgggcgc
     2041 aggccgcgca ggaagatatt tgtctgcgca gcaacccgcg taccgccagc ctggagcaga
     2101 ttgtcggcct gtacgcagcg gcgcaataaa tgccggatgc gacgcttgcc gcgtctgatc
     2161 cggccttcgg gattgcacat gtagggcgga taaggcgttt acgccgcatc cgccaataaa
     2221 taacaactaa cagggagtaa aggcgatggg aattaacgaa atcatcatgt acatcatgat
     2281 gttctttatg ctgatagctg ccgtagacag gatcctgtcg cagttcggcg gttctgctcg
     2341 tttcctcggt aagttcggta aaagtatcga aggatcaggc ggtcagttcg aagaaggctt
     2401 tatggcaatg ggcgcactgg gcctggcgat ggtcggtatg accgcgctgg caccggtact
     2461 ggctcacgta ctcgggccgg taattattcc ggtttacgaa atgctcggcg ctaacccatc
     2521 gatgttcgcc ggaacactgc tggcgtgcga tatgggcggc ttcttcctcg ccaaagagct
     2581 ggcgggcggc gacgtagcag cgtggctata ctctgggtta attctcgggt cgatgatggg
     2641 gccaacgatt gtgttttcca ttccggtggc gctcggcatt atcgaacctt ctgaccgtcg
     2701 ttatctggcg ctcggcgtgc tggcgggcat tgtgaccatt ccgattggct gtattgctgg
     2761 tggtctggtt gctatgtact ccggtgtgca gatcaacggt cagccggtgg aattcacttt
     2821 cgccctgatc ctgatgaaca tgatcccggt gatcattgtc gcgattctgg tggcgctggg
     2881 gctgaaattc atcccggaaa aaatgatcaa cggcttccag atcttcgcca aattcctcgt
     2941 ggcattgatc accctcggtc ttgccgctgc ggtagtgaaa ttcctccttg gctgggaact
     3001 gatccccgga ctggatccta tctttatggc ccctggcgat aaacccggtg aagtgatgcg
     3061 cgccattgaa gttatcggtt ctatctcctg cgtgctgtta ggggcgtatc caatggtgct
     3121 gctgctgact cgctggtttg aaaaaccgct gatgagcgtc ggtaaagtgc tgaatatgaa
     3181 caacatcgcg gcagccggca tggtggcaac gcttgccaac aacatcccga tgttcggcat
     3241 gatgaagcag atggataccc gcggcaaagt catcaactgc gccttcgccg tttccgctgc
     3301 tttcgccctg ggcgaccact taggcttcgc cgctgccaac atgaacgcca tgatcttccc
     3361 gatgattgtc ggcaagttga tcggcggcgt aacggcgatt ggcgtggcga tgatgctggt
     3421 gccaaaagaa gacgcgaccg cgactaaaac cgaagcggag gcacaatcgt gaacactcgc
     3481 cagctattga gcgtcggtat cgatatcggc accaccacca cccaggtgat tttctcccac
     3541 ctggagctgg ttaaccgtgc ggcggtgtcg caggtgccgc gctacgaatt cattaaacgc
     3601 gaaattagct ggcaaagtcc ggtgttcttt acccctgtcg ataaacaggg cggtttaaaa
     3661 gaagcggaac tgaaaacctt aatactcgag caatatcagg ctgcgggtat tgcgccggaa
     3721 agcgttgatt ctggtgccat catcatcacc ggtgaaagcg cgaaaacccg caatgctcgc
     3781 ccggcggtga tggcgctctc tcaatcgctg ggggattttg tcgttgccag cgccgggccg
     3841 cacctcgaat ccgtgatcgc cggtcacgga gctggggcgc aaaccctttc tgaacaacgg
     3901 ctgtgtcggg tactgaatat cgacatcggc ggtggcaccg cgaactacgc cctgttcgat
     3961 gccggaaaaa tcagcggcac tgcctgtctc aacgtcggtg gtcgcctgat ggaaaccgac
     4021 agccaggggc gcgtggttta cgctcataaa ccggggcaga tgattgtgga tgagtgcttc
     4081 ggtgcaggca ctgacgcccg ttcgctgacc ggcgcgcagc tggtgcaggt tacccggcgg
     4141 atggcggcgc tgattgtcga agtgattgac ggaacgcttt cgccgctcgc gcaggcattg
     4201 atgcaaaccg gtttgctgcc cgcaggtgtt acgcccgaaa tcattacgct ttctggaggc
     4261 gtgggcgaat gttatcgcca ccagcccgcc gacccgttct gttttgccga tattggccca
     4321 cttctggcaa cggcgctgca tgaccatccg cgcctgcgtg agatgaatgt gcagtttccg
     4381 gcgcaaaccg tacgcgccac ggtgattggc gcgggtgcac ataccctttc gctctctggc
     4441 agcacaatct ggctggaggg cgtacaactg ccgctgcgca atttgccggt ggcgatcccg
     4501 attgatgaaa cggatctggt gagtgcctgg caacaggcgc tgcttcagct ggatcttgat
     4561 cccaaaactg acgcgtacgt gctggcgctt cccgcctcgc tgcctgtgcg ttacgccgcg
     4621 gtactgacgg tcatcaacgc gctggtcgat ttcgtcgcgc gttttccgaa tccgcatccc
     4681 ctgctggtgg tggccgggca ggactttggt aaagctctgg gcatgttgtt gcgcccacag
     4741 ctacaacaac tcccgttggc agtcattgac gaagtgattg tccgcgcggg ggactatatc
     4801 gacattggta cgcctctttt tggcggatcg gttgtgccgg tgacggtgaa atcactcgca
     4861 tttccttcct gagggaacga cttatgaaac taaagaccac attgttcggc aatgtatatc
     4921 agtttaagga tgtaaaagag gtgctggcta aagccaacga actgcgttcg ggggatgtgc
     4981 tggcgggcgt tgcagcggca agctcacagg agcgcgtggc ggcaaagcag gtgttgtcgg
     5041 aaatgaccgt agcggacatc cgcaataatc cggtgattgc ctatgaagat gactgcgtga
     5101 cgcggctgat tcaggacgat gttaacgaaa cggcctacaa ccagattaaa aactggagca
     5161 tcagcgaact gcgtgagtat gtgctgagcg atgaaaccag cgtggacgac attgccttta
     5221 cccgcaaagg gctgacctcg gaagtggtcg cggcggtagc gaagatttgc tccaacgcgg
     5281 acctgatcta cggcgcgaag aaaatgccgg taatcaaaaa ggccaatacc accattggta
     5341 ttccgggcac cttcagcgcc cgtttgcagc cgaacgatac ccgtgacgac gtgcaaagta
     5401 tcgctgcgca aatctatgaa gggctttcct ttggggtggg cgatgcggtg atcggcgtta
     5461 acccggtaac tgacgacgtg gaaaacttaa gccgcgtgtt ggacaccatt tacggcgtga
     5521 tcgacaaatt caacatccca actcagggct gcgtactggc gcacgtcacc acccagatcg
     5581 aagcgattcg tcgcggcgcg cctggcggac tgattttcca gagtatctgt ggcagcgaaa
     5641 aagggctgaa agagtttggc gtggaactgg cgatgctcga cgaagcgcgc gcagtgggtg
     5701 cagagttcaa ccgtatcgcc ggggaaaact gcctctactt cgaaaccgga caaggctctg
     5761 cgctatccgc tggcgctaac ttcggcgctg accaggtgac gatggaagca cgtaactatg
     5821 ggctggcgcg tcattacgat ccgtttatcg tcaacaccgt ggtcggcttt attgggccgg
     5881 agtatctcta caacgaccgc cagattatcc gtgctggctt agaagatcac tttatgggca
     5941 agctgagcgg catctctatg ggctgtgact gctgttacac caaccacgct gacgctgacc
     6001 agaacctcaa cgaaaacctg atgatcctgc tcgccaccgc aggctgcaac tacatcatgg
     6061 ggatgccgct gggtgatgac atcatgctca actaccagac caccgcattc cacgataccg
     6121 ccactgtgcg tcagttactc aacctgcgtc cgtcaccgga gtttgaacgc tggctggaaa
     6181 gcatgggcat tatggcaaac ggtcgcctga ccaaacgggc gggcgatccg tcactgttct
     6241 tctgatgacg cggagataac acatcatgga tcaaaaacag attgaagaaa ttgtacgcag
     6301 cgtgatggcg tcaatgggac aaacggcccc cgcgccgtca gaagcaaagt gcgccaccac
     6361 caactgtgcg gcaccggtga cctcggaaag ctgcgcgctg gatttaggtt ctgcggaagc
     6421 aaaagcgtgg attggtgttg aaaacccgca tcgcgcagac gtattaacag aactgcgtcg
     6481 cagcaccgtg gcccgcgttt gtaccggtcg tgccggtccg cgtccgcgta cgcaggcgtt
     6541 gctgcgtttc ctggccgatc actctcgttc gaaagatacc gtactgaaag aagtgccgga
     6601 agagtgggtg aaagcgcagg gcttgctgga agtacgctcc gaaattagcg acaaaaatct
     6661 ctacctgact cgcccggata tgggccgccg cctgtgtgca gaagctgttg aagcgctgaa
     6721 agcacagtgt gttgctaatc cggacgtaca ggtcgttatt tctgatggcc tgtcaactga
     6781 tgccatcacc gtgaactacg aagagatcct gccgccgctg atggcgggcc tgaaacaggc
     6841 cgggctgaaa gtcggcacgc cattctttgt tcgttatggt cgcgtgaaga ttgaagatca
     6901 gatcggtgag atcctcggcg cgaaagtggt gatcctgctg gtgggggaac gtccggggtt
     6961 agggcagtca gaaagcctct cctgctacgc cgtttactcg ccgcgtatgg cgaccaccgt
     7021 tgaggccgat cgcacctgta tctctaacat tcaccagggc ggcacgccgc cggttgaagc
     7081 cgcagccgtc attgtggatt tggcgaaacg tatgctggag cagaaagcat ccggcatcaa
     7141 catgacccga taaggaggca tcatgccagc tttagatttg attcgaccgt cggtaaccgc
     7201 catgcgggtg attgcctccg ttaacgctga atttgcgcgt gaactaaaat tgccgccgca
     7261 tattcgtagc ctcgggctga tttctgccga ttctgacgac gtcacctaca ttgcggctga
     7321 cgaagcgacc aaacaagcga tggtcgaagt ggtatatggt cgctcgctgt atgcgggagc
     7381 ggcacacggc ccgtcaccga ctgccggtga agtgctgatt atgcttggtg ggccaaaccc
     7441 ggcggaagtg cgtgccggtc tggatgcgat ggttgcacat attgaaaatg gcgcagcttt
     7501 ccagtgggcc aatgacgcag aaaacaccgc cttcctggca catgtagttt cgcgtaccgg
     7561 ttcttatctc tcatcaaccg ccgggatcac gcttggcgat ccgatggcgt atctggtggc
     7621 accgccgctg gaagcgacct acggcattga tgcagcgttg aaatctgccg acgtacagct
     7681 ggtgacctat gtcccgccac cgtctgaaac caactactcg gcagcatttt taaccggtag
     7741 ccaggccgcg tgtaaagcag cctgtaacgc ctttaccgat gcagtgctgg aaatcgcgcg
     7801 taatccaatc cagcgtgcgt aacggaggtt gccgatgatc aatgcactgg gattgctgga
     7861 agtggacggc atggtcgccg cgatagatgc agcggatgcc atgctcaaag cagctaacgt
     7921 tcgtctgctc agtcacgaag tgcttgaccc tggtcgctta acgctggtgg tggaaggcga
     7981 tctggcggcg tgtcgtgcgg cgctggacgc aggttgtgct gccgcgatgc gtaccgggcg
     8041 tgtcatcagc cgcaaggaga tcggtcggcc agacgatgac acccagtggc tggttactgg
     8101 ctttaaccgc cagccgaagc aacccgtaag ggaacccgac gcgccagtta tcgtcgcgga
     8161 atctgctgac gagttgttgg cgctgttaac atcagtacgt cagggaatga cggcaggaga
     8221 agtggctgcc cactttggct ggccgctgga aaaagccaga aatgcgctcg aacagctctt
     8281 ttctgccggg acgttacgta aacgcagtag tcgttatcgt ctcaagcccc attaacctgt
     8341 cggaggtgcc gggtgtccta caacacccgg cattaacatc atgaaaaaga cccgtacagc
     8401 caatttgcac catctttatc atgaaccctt acccgaaaac ctgaagctca cgccgaaggt
     8461 cgaagtggat aatgttcatc aacgacagac aacggatgtc tatgaacatg ctttaacgat
     8521 taccgcctgg cagcagattt acgatcagct gcatccgggc aagtttcatt gtgaatttac
     8581 ggaaattcta ctcgatgata ttcaggtttt tcgtgaatac accggtctgg cgctgcgtca
     8641 gtcgtgcctg gtctggccga actcgttctg gtttggcatt ccggcgacgc gcggtgagca
     8701 gggatttatc ggttcgcaat gtctgggaag cgcggaaatc gccacccgcc ctggtggcac
     8761 tgaatttgaa ctgagcacgc cggatgatta cacgatcctg ggcgtggtgc tttctgaaga
     8821 tgtcatcacc cggcaggcta actttttgca taacccggat cgggtattac atatgctgcg
     8881 tagccagtcg gcgctggaag tgaaagagca gcataaagcc gcgctgtggg gctttgtcca
     8941 acaggcgctg gcgacgtttt gcgagaaccc ggaaaatctc catcagcctg cagtgcgaaa
     9001 agtgctgggg gataatttgc taatggcgat gggggcgatg ctggaagacg cgcaaccaat
     9061 ggtgacggcg gaaagcatca gtcatcagag ttaccgtaga ttactttccc gcgcccgtga
     9121 atatgtgctg gaaaatatgt ctgagccggt gacggtgctg gacttgtgta atcaactgca
     9181 tgtcagtcgc cgcacgctac aaaacgcgtt tcacgctatt ttaggcattg gcccaaacgc
     9241 gtggctgaaa cgcattcgcc tgaacgccgt acgccgcgaa ctgataagcc cgtggtcgca
     9301 aagcacaacg gtaaaagacg ccgccatgca gtggggattc tggcatctgg ggcaatttgc
     9361 cacggattac cagcagctgt ttgccgagaa gccgtcgttg acgttgcatc agcggatgcg
     9421 ggaatggggg tgagggagtt acacccaatc cctgacctta ataaactcac ttaacgccgc
     9481 ttctgggctg ccatcttttg gctgataatc atactcccag cgtaccagtg gcggcattga
     9541 catcaggatc gactcggtgc gcccgccagt ttgcaggcca aacagagtgc cgcgatccca
     9601 gaccagattg aactcgacat aacgaccgcg acggtagagc tgaaaattgc gctcgcgctc
     9661 gccgtaggcc atcgctttac gtcgctctac aattggtaaa taagcgtcgg tgtagccttt
     9721 gcctatcgcc tgcataaagg caaaacagtg gtcgaaatct ggcgtgttca gatcatcaaa
     9781 gaacagcccg ccaataccgc gctgttcgtt gcgatgtttg aggtagaagt aatcgtcgca
     9841 ccactttttg taacggggat aaacgtcttc accaaatggc aggcacaggt cacgggcggt
     9901 gcggtgccag tgaatggcgt cttcttcaaa accatagaaa ggggttaaat cgaagccgcc
     9961 gccaaaccac cagacgggct ctgcacccgg tttttcggca ataaaaaaac gcacattcgc
    10021 gtggctggtg ggaacatacg ggttatgcgg atgcactacc agtgaaacgc ccatcgcctc
    10081 gaaactgcgc ccggcaagtt ccgggcgatg agcggtggcg gaagctggca tcgcctcgcc
    10141 gtggacgtga gaaaagttga cgcctgcctg ttcgaaaaca ccaccattac gcaacacccg
    10201 actacgcccg ccgccgccag cttcgcgctg ccaactatct tcgacaaatt ctgcgccatc
    10261 gacggcggtc agctgctgac aaatcgtatc ctgaaggttg agcagaaact gtttaacctg
    10321 gtgtgcgtcg ggtttcataa cttatcgctt tttcgaatgt gctttctggt tgtcgaacca
    10381 gtggaaataa ctgatcacgc cttcagcaat cgctgtggcg attttctgac gaaacgccgc
    10441 cgtgcctaac agccgctctt cttccgggtt ggtgataaac gaggtttcca ccagcaccga
    10501 aggaaccgac ggcgatttca acaccacaaa cgcggcttgt tcggtgttgc ggctgtgcag
    10561 tttatgcacc ggcttaatct ttttcagaat atgcgagccg agcgtcaggc tgtttttaat
    10621 agtatcggtt tgcaccagat caaacagcac ttgctgcaat aggtgatcct tgtcagtcgc
    10681 ctttttaccg gcaacttcat cggcgcggtt ttcgcgttca gacaggtatt tcgccattgc
    10741 gctacttgcc ccacggttag agagggcaaa taccgaagca ccggcagctt tcgggttggt
    10801 aaagccatcg gcatgaattg acataaacag atctgcgcca tgtttatggg cgatttcaac
    10861 gcgatcgtaa agtgggataa acgtatcgcc agaacgcgtt aaacgcgcat caatcccatg
    10921 attacgcaaa atggaacgga cgtttttagc aatcgccagc accacatgtt tttctttcga
    10981 accgttgcgt ccgatcgctc cggtatcaat tccgccgtgg cctggatcga gaacaacgac
    11041 acgtttgccg ccagattttt tggctttcgg cttgctgtgt ccgttgctgg tttttaaagg
    11101 ttcgtctttg gcgatggctt gcgacattcc tgacaacgtc agggcagcca aaccggcttt
    11161 cagcacctgg cggcgcgaag tgagtgtttt tagtggttta aaagtgctca tacggcctga
    11221 gttgtaataa taaagttcca gatgttatat cgtatcgcgt aatccgttac gacggtttga
    11281 tgttgagaat tgttttactt ttcatttcaa tagttgacaa ctccccatta tgccactttt
    11341 tcaaccgatt ttcgtcagat attggctaat tcgaccgttc acgccataat cagcctttta
    11401 acccgcaaaa aggtaacgaa taccatggag atacgcgtat ttcgccagga agatttcgaa
    11461 gaggtcatca ccctttggga gcgttgcgac ttgctgcgtc cgtggaacga tccggaaatg
    11521 gacatcgagc gtaagatgaa ccatgacgtc agtttgtttt tggtcgctga ggtaaacggt
    11581 gaagtggtcg gaacggtgat gggcggttat gacgggcatc gcgggtctgc ttattatctt
    11641 ggcgtgcatc cagagtttcg tgggcgtggg attgccaatg cgttgcttaa tcggctggag
    11701 aaaaagctga ttgctcgtgg ctgcccgaaa attcagatca atgtgccgga agataacgac
    11761 atggtgctcg gaatgtatga acgcctggga tatgaacacg ccgacgtgct gagtctgggt
    11821 aagcgtttga ttgaagatga agagtactga gttccatcct gtccattatg atgcgcacgg
    11881 acgcctgcgt ttacccttgc tcttctggct tgtgctattg cttcaggcgc gaacctgggt
    11941 gctgtttgtc attgccggtg cgtcgcgcga gcagggtact gcgctgctaa atctgtttta
    12001 tcccgatcac gataatttct ggctggggct gattccaggc attccggcgg tgctggcgtt
    12061 tttgttgagc ggtcggcggg ctacatttcc tcgcacttgg catgtgctct attttctgtt
    12121 gctgttggcg caggtggttt tactttgctg gcaaccgtgg ctgtggctga acggtgaatc
    12181 cgttagcggt atcggtctgg cgctggtagt ggcggatatt gtggcgttaa tctggctgct
    12241 gaccaatcga cgtttacgcg cttgttttaa tgaagaaaaa gaataacggc actttttggt
    12301 gaatttgcac tccaagcaac gttattgaat aaccaaagga agtgacatga aatcgctgcg
    12361 tttaatgtta tgcgctatgc cgttgatgct gaccggctgt tccacgatgt cgtcagttaa
    12421 ctggtcggcc gctaacccgt ggaactggtt tgggtcgtcc accaaagtga gcgagcaggg
    12481 cgttggtgaa ttaacggcgt ccacaccact gcaagaacaa gccattgccg atgcgcttga
    12541 tggcgattat cgcctgcgca gcggaatgaa aaccgcgaac ggcaacgtgg tgcgcttttt
    12601 tgaagtgatg aagggcgaca acgtggcgat ggtgattaac ggcgatcaag gcacgatcag
    12661 tcgcattgac gtgctggata gcgatattcc tgctgacacc ggtgttaaaa tcggtacacc
    12721 gtttagcgac ctttacagca aagcatttgg caattgccaa aaagctgatg gtgatgataa
    12781 tcgtgccgtc gaatgtaaag ccgaaggcag tcaacatatt agctaccagt tcagcgggga
    12841 atggagtggt cctgaagggt taatgccttc ggacgatacc ctgaaaaact ggaaagtcag
    12901 taaaattatc tggcggcgtt aatttgcgtc tgaacaaacc gccgccgcga aaaaacacgt
    12961 aaaatagcgc tcaacaatgc cacggattgc gtggcattat tattttcagg aggaacaatg
    13021 tctcaggttc agagtggcat tttgccagaa cattgccgcg cggcgatttg gatcgaagcc
    13081 aacgtgaaag gggaagttga cgccctgcgt gcggccagta aaacatttgc cgacaaactg
    13141 gcaacttttg aagcgaaatt cccggacgcg catcttggtg cggtggttgc ctttggtaac
    13201 aacacctggc gcgctctgag cggcggcgtt ggggcagaag agctgaaaga ttttccgggc
    13261 tacggtaaag gccttgcgcc gaccacccag ttcgatgtgt tgatccacat tctttctctg
    13321 cgtcacgacg taaacttctc tgtcgcccag gcggcgatgg aagcctttgg tgactgcatt
    13381 gaagtgaaag aagagatcca cggcttccgt tgggttgaag agcgtgacct gagcggcttt
    13441 gttgacggta cggaaaaccc ggcgggtgaa gagacgcgtc gcgaagtggc ggttatcaaa
    13501 gacggcgtgg atgcgggcgg cagctatgtg tttgtccagc gttgggaaca caacctgaag
    13561 cagctcaacc ggatgagcgt tcacgatcag gagatgatga tcgggcgcac caaagaggcc
    13621 aacgaagaga tcgacggcga cgaacgtccg gaaacctctc acctcacccg cgttgatctg
    13681 aaagaagatg gcaaagggct gaagattgtt cgccagagcc tgccgtacgg cactgccagt
    13741 ggcactcacg gtctgtactt ctgcgcctac tgcgcgcgtc tgcataacat tgagcagcaa
    13801 ctgctgagca tgtttggcga taccgatggt aagcgtgatg cgatgttgcg tttcaccaaa
    13861 ccggtaaccg gcggctatta tttcgcaccg tcgctggaca agttgatggc gctgtaagcg
    13921 tttctgattc attgattaaa ggccaggcag taacattact gactggcctt tttattactt
    13981 ttgctttaac gccgcataca cctgatcaac tacccaacca tacgttgcaa tcggcagctg
    14041 accgctttcg aacatattgg gattcttttg cggatcggca accggcgaat gtggcttgtt
    14101 gcttaacatc acaatcgcca tatgattcac cggatcgata acggtcaccg ttccggtcca
    14161 gccagtgtga ccgtaggttt gcgggcttgc cagcgtgcca aacgtcggcg tcatggtggc
    14221 attaccattc acgcgccagc cgaggccaaa agtggcatct tccttagagc tggtggtgaa
    14281 catcttcacc gtttccgcac tgaacagctg cacatcgcca tagcccccgc cgttcagcat
    14341 cgtttgcatt aacaccgcaa tatcgccggt attggaaaac aaacctgcgt gcccggaaac
    14401 gccgcccatc gaataaaagg ctttttcatc gtgcacctga ccccagagag tggaggtgcg
    14461 gatattcggg aaatggatca cgccatcgcg ggtattgccg ttcaattccg tggcggcaat
    14521 ttgctgcggt ttaaagcctt tcagcagcgg gttaaatacc gtatgtgtca ggccgagcgg
    14581 gcgataaatc gactcttcaa catagcggtc aagcggctga ccggtgaccg actcgacgat
    14641 aaatccaagc agcatataat cgacatcact gtaaatatgc ttgctgccgg gctgatactc
    14701 cagcggcgtg cgcttgatca tctccagcgt ctggccttta tcctgggaat ataacgcgcc
    14761 cgcgaccgct ttattcggat attgtgggtc cgccgggaaa ccgccgctgt gatgcagcag
    14821 gtcagaaatc cgcagcgtgt ttttgccttt gatggtgtca tttggactat cggcaaaccc
    14881 cggaatatat ttcgcaatcc gatcgtcagg atgcagtttg ccttcgctca tcagcttttg
    14941 cagggcgaag ttcgtggcgt acattttggt gtttgaggcc aggtcataca gcgtcccggt
    15001 ggtggctttg acaggctgtt ccatcaacac gctgccatcg tactttttcg ccgctcccca
    15061 ggctttgcga tacacaatct gattatcttt aatgatcagc aggtttacgc cgggataacc
    15121 ggcgtccact tgctggctaa tccagcgatc catctggtta agccgttcga cgttgaaccc
    15181 tgctttctct ggcgaacttt ctgtcagaac agggtatttt gcggcgctga cgctacaact
    15241 aacagccagc agagaaagat agagcattgt ccgtttcatg atatcagtcc agattgacgt
    15301 tacggcagcc aaagagcgtg gtgaaaataa acccgcaaac ccatgccacc agaatcccgc
    15361 cagcataaac cgccattgcc ggaaggatgc cttgtgcgga agtcatcagc ggcagcgcca
    15421 ccagaccaga cggcccaaag gcgctgttta agcccatcgg cagaccccac caggctatca
    15481 ggccgataaa caaaccgccc gctgcgccgc ctaaacaggc ggtaataaac ggtttcatgc
    15541 ggggcagggt gacgccgtaa atcaacggtt caccaacgcc cagcagaccg ggaataatcg
    15601 ccccgcgtac ctgactgcgt aatgcactgt gcggttgcgc ccgccagtag agtgccagcg
    15661 ccgcgcccac ctggcccgcg cctgccattg aaaggatggg aaataagctg ttgaatccct
    15721 ggctgtccat taacgcgagg taaacaggaa taaagccctg atgcacgcca aataccacgg
    15781 cgatcaggaa cagcccggct aaaaccgcac aaccgaacgg attactgttc aggtgcataa
    15841 acagccatga cataccttcg aataaccagc cacccagcgg cataatgatc aggtacgcaa
    15901 gcgtagcggt gatcagcagg gtgattaacg aggtcagcag catgtcgaga tcgtccggca
    15961 taaagcggcg caccatgcct tcaatgcggg cgcaggccca cgcggcaatc agcacgccga
    16021 taatattgcc gcgcggatcg atgggcagac caaagaaatc gtgaaaaccg gcgtagtaac
    16081 cggtggttgc agcggggtta taaccgagca aaaagagtgc ggcaataatt gcgccattga
    16141 cgcccgtgcc gccaaatgcc tgggcggcgt tatagcccac cagaatcacc aggaaagtga
    16201 acaaaccttt gctgaacacc ttcataaaat tcagcgcgtc gggtagcgtg ccctgagcat
    16261 ctgccggaac gtgcatcacc gtggcaatta acgtcgctat ccccagcagc agaccggcgg
    16321 caataaaacc ggggatcagc ggcgtgaaga tggtggcgaa tttggcgaga aattgttgca
    16381 cgccggaggt ttgtttggct tttaactgac gtttgttctg ggcggcgatt tctgcggcat
    16441 cctgtaccgg tgcctcgccc agcagctcgc tcatcgcttt tgcggcacga tgcgctttac
    16501 caggcccaaa aacgacctgc acctggtcgc tggtcaaaat gacgcccttc acgccttcca
    16561 gcgttttgat attgggatca accagtgaac tgtcatgtac acccagacgc aggcgcgtca
    16621 tacagttacc acaactggcg atatttcccg gtccgccgac acgggtaaga atggtgttca
    16681 gaagttcact gctgatctct ttggccatgg gttattcctt gtctaaaacc tgacgaataa
    16741 agccgccgtg ttgatccagg cgttttttag cttctgcggc atcgagattt ttcagcacca
    16801 tcacaatggc cgttttacag ttgcgctcgc aggcaattaa cgccgcttcc gcttgttcgg
    16861 cattacatcc ggtggcgttt ttaacgatat tgacctggcg cacatgcagt ttttcgttgg
    16921 tggcgaccac atcgaccatc aggttgccga acactttgcc agatttaatc atcagcccgg
    16981 tggaaagcat attgagcacc agtttctgcg ctgtgcctgc tttcattcgt gaagaaccag
    17041 tgaccacttc ggcacctaca atcggtgtaa tggcaaactc ggcggtggtg gaaacggcac
    17101 tccccggatt acaggaaatt cccactgtgc ggcagccgag ctggcgtgcg tattccagtc
    17161 cggcaatcac atacggcgtg cgaccgctgg cagcaatgcc aaccaccaca tcctgtgccg
    17221 ttaaattaat atttttcaga tcgttaacac caccttcccg gctatcttca gcgccttcca
    17281 ccgcgtgctg aatggcatat tcgcctccag caatcaaacc aaccaccaga cccggtttca
    17341 cgccgtaggt gggcggacat tcgctggcat ccagaatccc cagacgaccg gatgttcccg
    17401 caccgagata aatcagacga ccgccgccgc tgacctgggc gtggataaca tcgatcgccg
    17461 cggcgatatc cggcaggacg cgctcaacgg caagcggtac ggttttatct tcatcgttga
    17521 taatccggca catttccagc gtcgatacgc ggtcaatttc agccgaggcg gtgttcgagc
    17581 cttcagtaat catcttttct agttgcatcg gtggtgacct tactatttac gatcaaataa
    17641 ttgcagatgt atggaatcat atatcccgaa ttgacagact taaaggaatc attgagtcta
    17701 cactcgcaga gtgtgaagag cgtcataaga acttaagcgg aatttatgct gccagcccag
    17761 gagagtggaa gatgttgtac ctgacgaaaa taagcaatgc ggggagtgaa ttcaccgaaa
    17821 acgagcaaaa aatcgctgac tttctgcagg ccaacgtgag tgagctgcaa tccgtctctt
    17881 cgcgcaagat ggcgaaacag ctcggcatca gccagtcgag tattgtgaag tttgcccaga
    17941 agctcggcgc acaaggattt actgaattgc gtatggcgtt aattggcgaa tacagtgcca
    18001 gccgggagaa aacgaatgcc acggcgctgc acctgcacag ttcgattacc agtgatgatt
    18061 cgctggaagt gattgcccgc aaactgaatc gcgaaaaaga gctggcgctg gagcaaacct
    18121 gcgcgttgtt cgactacgcg cggctgcaaa aaatcattga tgtcatcagt aaagcgcaat
    18181 ttattcagat aacgggcctg ggcggatcgg cgctggtggg gcgcgatctg tcgttcaaac
    18241 tgatgaaaat tggatatcgc gtggcctgcg aagccgacac gcacgtacaa gccaccgttt
    18301 cccaggcgtt gaaaaaaggc gatgtacaaa tcgctatctc ttacagcggc agtaaaaaag
    18361 agattgtctt gtgcgcagaa gcggcacgaa agcagggggc aacggtgatt gccattactt
    18421 ctctggctga ctctccgctg cggcggctgg cgcatttcac gctcgatacc gtttccggtg
    18481 aaaccgaatg gcgtagctcg tcgatgtcta cccgcacggc gcaaaattct gttaccgatt
    18541 tactgtttgt tggtcttgtg caactcaatg atgtcgagtc attgaaaatg attcagcgca
    18601 gcagtgagct aacacaacgc ctgaaatgat gcataaagca gcgactggat tgagattttc
    18661 ctgaattagt gagctgatcc gcagcaatat tttgtttatc ctgtattttc agagggaatg
    18721 gagtgtaacg ctctgtatta acaaggagag cattaaaatg ggtaaactca cgggcaagac
    18781 agcactgatt acgggcgcat tgcagggaat tggcgaagga attgccagaa cttttgcgcg
    18841 tcatggcgca aacctaatct tgctggatat ctcccctgag atcgaaaagc tggcggacga
    18901 actgtgtggt cgtggtcatc gctgtacggc ggttgtcgcc gatgtgcgtg acccggcgtc
    18961 ggtagccgca gctatcaaac gcgcgaagga aaaagaaggg cgcattgata tcctggtgaa
    19021 taacgcaggc gtttgtcgtc tgggcagttt cctcgatatg agcgatgaag atcgcgattt
    19081 ccatattgac atcaatatta aaggcgtatg gaacgtcacg aaggcggtgc tgccagagat
    19141 gattgcgcgc aaagatggtc gcattgtgat gatgtcttca gtcactggtg atatggtggc
    19201 cgatccgggt gaaacggcgt acgccttaac gaaagcggcg attgttggcc tgacaaaatc
    19261 gctggcggtg gagtacgcgc aatccggtat tcgcgttaac gccatctgcc cggggtacgt
    19321 ccgcacgcca atggcggaaa gcattgcccg ccagtcgaac ccggaagatc cagagtcggt
    19381 gctgactgat atggcaaaag caatcccgct gcgtcgcctc gccgatccgc tggaagtcgg
    19441 cgaactggcg gccttcctcg catcggatga atccagctat ttaaccggta cacagaatgt
    19501 gattgatggc ggcagcacac tgccggagac ggttagcgtc ggtatctgat tcacctctgt
    19561 ttcctccctg catgtgtggg gaggatttcg ttttaaaccc ggttcttatt cccttttcaa
    19621 cttccaaatc accaaacggt atataaaacc gttactcctt tcacgtccgt tataaatatg
    19681 atggctatta gaaagtcatt aaatatataa gggtgcgcaa tggccgttaa cttactgaaa
    19741 aagaactcac tcgcgctggt cgcttctctg ctgctggcag gccatgtaca ggcaacggaa
    19801 ctgctgaaca gttcttatga cgtctcccgc gagctgtttg ccgccctgaa cccgccgttt
    19861 gaacagcaat gggccaaaga taacggcggc gacaaactga cgattaaaca atctcatgcc
    19921 gggtcatcaa aacaggcgct ggcgatttta cagggcttaa aagccgacgt tgtcacttat
    19981 aaccaggtga ctgacgtaca aatcctgcat gacaaaggca agctgatccc ggcggactgg
    20041 cagtcgcgcc tgccgaataa tagctcgccg ttctactcca ccatgggctt cctggtgcgt
    20101 aagggcaacc cgaagaatat ccacgactgg aacgacctgg tgcgctccga cgtgaagctg
    20161 attttcccga acccgaaaac gtcgggtaac gcgcgttata cctatctggc ggcatggggc
    20221 gcagcggata aagctgacgg tggcgataaa gccaaaaccg aacagtttat gacccagttc
    20281 ctgaaaaatg ttgaagtgtt cgataccggc ggtcgtggcg caaccaccac ttttgccgag
    20341 cgtggcctgg gcgatgtgct gattagcttt gaatcggaag tgaacaacat ccgtaaacag
    20401 tatgaagcac agggctttga agtggtgatc ccgaaaacca atattctggc ggaattcccg
    20461 gtggcgtggg tcgataaaaa cgtgcaggcc aacggtacgg aaaaagcagc aaaagcctac
    20521 ctgaactggc tctacagccc gcaggcgcag accatcatca ccgactatta ctaccgcgta
    20581 aataacccgg aagtcatgga caaactgaaa gataaattcc cgcagaccga gctgttccgc
    20641 gtggaagaca aatttggctc ctggccggaa gtgatgaaaa cccacttcac cagcggcggc
    20701 gagttagaca agctgttagc ggcggggcgt aactgatgtt tgcagtttca tcaagacgcg
    20761 tgctgccggg ctttacctta agcctcggca ccagtctgct gtttgtgtgc ctgattttgc
    20821 tgctgccgct ctccgcgctg gtgatgcagc tggctcagat gagctgggcg cagtactggg
    20881 aggtgatcac caacccacaa gtggtcgcgg cctacaaagt gacgctgctg tcggcgtttg
    20941 tggcatcgat ttttaacggc gttttcggtc tgctgatggc gtggatttta acacgttatc
    21001 gctttccggg ccgcacgctg cttgatgcgc tgatggattt accctttgcg ctgccaacgg
    21061 cagtcgccgg tttaacgctg gcctcgctct tttccgtaaa cggtttttac ggtgaatggc
    21121 tggcgaagtt tgatatcaaa gtcacctata cctggcttgg tattgcggtg gcgatggcct
    21181 ttaccagcat tccgtttgtg gtgcgtaccg tgcagccggt gctggaagag ttaggcccgg
    21241 aatatgaaga agcggcggaa acgcttggtg caacgcgctg gcagagtttc tgcaaagtgg
    21301 tgctgccgga actttctcca gcgctggtgg cgggcgtggc gttgtcgttt acccgtagtc
    21361 ttggtgagtt tggcgcggtg atatttatcg ccgggaacat cgcctggaag acggaagtga
    21421 cgtcgctgat gatttttgtg cgcttgcagg agtttgatta cccggcggcg agcgcgattg
    21481 cttcggtgat cctcgcggca tccctgctgc tgctgttctc aattaacact ctgcaaagtc
    21541 gctttggtcg gcgtgtggta ggtcattaat ggcggaagtt acccaattga agcgttatga
    21601 cgcgcgcccg attaactggg gcaaatggtt tctaattggc atcgggatgc tggtttcagc
    21661 gttcatcctg ctggtgccga tgatttacat cttcgtgcag gcattcagca aggggctgat
    21721 gccggtttta cagaatctgg ccgatccgga catgctgcac gccatctggc tgacggtgat
    21781 gatcgcgctg attgccgtac cggtaaacct ggtgttcggc attctgctgg cctggctggt
    21841 gacgcgcttt aacttccctg gacgccagtt actgctgacg ctactggaca ttccgtttgc
    21901 cgtatcgccg gtggttgccg gtctggtgta tctgctgttc tacggctcta acggcccgct
    21961 gggcggctgg ctggatgaac ataatctgca aattatgttc tcctggcccg gaatggtgct
    22021 ggtcaccatc tttgtgacgt gtccgtttgt ggtgcgcgag ctggtgccgg tgatgttaag
    22081 ccagggcagc caggaagacg aagcggcgat tttgcttggc gcgtctggct ggcagatgtt
    22141 tcgccgcgtc acattgccga acattcgctg ggcgctgctt tatggcgtgg tgttgaccaa
    22201 cgcccgcgca attggcgagt ttggcgcggt gtcggtggtt tccggctcga ttcgcggcga
    22261 aaccctgtcg ctgccgttac agattgaatt gctggagcag gactacaaca ccgtcggctc
    22321 ctttaccgct gcggcactgt taacgctgat ggcgattatc accctgtttt taaaaagtat
    22381 gttgcagtgg cgcctggaga atcaggataa acgcgcacag caggaggaac atcatgagca
    22441 ttgagattgc caatattaag aagtcgtttg gtcgcaccca ggtgctgaac gatatctcac
    22501 tggatattcc ttcaggtcag atggtcgcgt tgctggggcc gtccggttcc gggaaaacca
    22561 cgctgctgcg cattatcgcc gggctggagc atcaaaccag cgggcatatt cgcttccacg
    22621 gcaccgacgt cagccgcctg cacgcgcgcg atcgcaaggt cggtttcgtg ttccagcatt
    22681 acgcgctgtt ccgtcatatg acggtgttcg acaatatcgc ttttggcctg acggtgctgc
    22741 cacgtcgcga gcgcccgaat gccgcagcca tcaaagcgaa agtgacaaaa ttgctggaga
    22801 tggtgcagct tgctcatctg gcagatcgtt atccggcaca gctttccggc ggccagaaac
    22861 agcgcgtggc gctggcgcgc gcgctggctg tagaaccgca aattctgctg cttgatgaac
    22921 cgtttggcgc gctggatgcg caggtgcgta aagagctgcg tcgctggctg cgtcaactgc
    22981 atgaagagct gaaattcacc agcgtgttcg tgacccacga ccaggaagag gcgaccgaag
    23041 tagccgatcg tgtagtggtg atgagtcagg ggaatattga acaggctgat gcgccgaatc
    23101 aggtatggcg cgaaccagcg acccgttttg tactcgaatt tatgggcgaa gtgaaccgcc
    23161 tgcagggaac cattcgcggc gggcagttcc atgttggcgc gcatcgctgg ccgctgggct
    23221 acacacctgc gtatcagggt ccggtggatc tcttcctgcg cccgtgggaa gtggatatca
    23281 gccgccgtac cagtctcgat tcgccgctgc cggtacaggt actggaagcc agcccgaaag
    23341 gtcactacac ccaattagtg gtgcagccgc tggggtggta caacgaaccg ctgacggtcg
    23401 tgatgcatgg cgacgatgcc ccgcagcgtg gcgagcgttt attcgttggt ctgcaacatg
    23461 cgcggctgta taacggcgac gagcgtatcg aaacccgaga tgaggaactt gctctcgcac
    23521 aaagcgcctg ataggttgag tgaatgttaa acgcccggag gcgcttcccg cgatccgggc
    23581 tttttaatgg caaggtttgt aacctgtaga cctgataaga cgcgcaagcg tcgcatcagg
    23641 caacaccacg tatggataga gatcgtgagt acattagaac aaacaatagg caatacgcct
    23701 ctggtgaagt tgcagcgaat ggggccggat aacggcagtg aagtgtggtt aaaactggaa
    23761 ggcaataacc cggcaggttc ggtgaaagat cgtgcggcac tttcgatgat cgtcgaggcg
    23821 gaaaagcgcg gggaaattaa accgggtgat gtcttaatcg aagccaccag tggtaacacc
    23881 ggcattgcgc tggcaatgat tgccgcgctg aaaggctatc gcatgaaatt gctgatgccc
    23941 gacaacatga gccaggaacg ccgtgcggcg atgcgtgctt atggtgcgga actgattctt
    24001 gtcaccaaag agcagggcat ggaaggtgcg cgcgatctgg cgctggagat ggcgaatcgt
    24061 ggcgaaggaa agctgctcga tcagtttaat aatcccgata acccttacgc ccattacacc
    24121 actaccgggc cggaaatctg gcagcaaacc ggcgggcgca ttacccattt tgtctccagc
    24181 atgggaacaa ccggcactat caccggcgtt tcacgcttta tgcgcgaaca atccaaacca
    24241 gtaacgattg ttggcctgca gccggaagaa ggcagcagta ttcccggcat tcgccgctgg
    24301 cctgcggaat atctgccggg gattttcaac gcttctctgg tggatgaggt gctggatatt
    24361 catcagcgcg atgcagaaaa caccatgcgc gaactggcag tgcgggaagg aatattctgt
    24421 ggcgtcagct ccggcggcgc ggttgccgga gcactgcggg tggcaaaagc taaccctggt
    24481 gcggtggtgg tagcgatcat ctgcgatcgt ggcgatcgct acctttccac cggggtgttt
    24541 ggggaagagc attttagcca gggggcgggg atttaagatt attgcgcagg caccgtttta
    24601 tccgtctgcg caattagcgc atgtaaaaaa ggctgtgcgt tctcatcgct cttaccgggg
    24661 atcttcacaa tatacggctt cccggtaatc gatgacgacg aagcaacctt atcaataaac
    24721 tgctcggcag tgtcaatgcg gttacgggta ttgcccagct tcagacgcag atgagaaacc
    24781 gcttcatcgc aggtatgttc atcaccgttg cgcacaaata tcaaatcctt tttctgtgct
    24841 aatccctcca gcatggcgtt gatgcgggct tcttcatggg cagttaactt cgcgtaagcg
    24901 gggagtgtca ttagtagtgt aatgaccaga caaatgattt tcttcattga attaccttat
    24961 ccttgttttg tgatagccga tttgactgcc agaatcaggt aaagtttcgg ttggaatacg
    25021 atgaggatca tcggcaagga gaaaaaatta aatgagtagt ttgttgttgt ttaacgataa
    25081 gagcagggcg ctgcaggcgg atatcgtcgc cgtgcagtcg caggtggttt acggcagcgt
    25141 gggcaacagc attgccgtgc ctgctatcaa acagaacggt ctgaatgttt ttgccgtgcc
    25201 gacggtattg ctgagcaata cgccgcatta tgacactttc tacggtggtg cgattccgga
    25261 tgaatggttt agcggttatt tgcgtgcgct tcaggagcgt gatgcgctgc gccagctgcg
    25321 cgctgtaacc acgggctata tgggaacggc atcgcaaatc aaaatccttg ccgagtggct
    25381 gactgcgcta cgcaaagacc atcctgacct attgatcatg gtcgatccgg tgattggcga
    25441 tattgatagc ggaatttatg tcaaacctga ccttcccgag gcgtatcgac aatatttact
    25501 gccgctggcg cagggaatta cccccaatat ctttgagctg gaaatcctga ccggtaaaaa
    25561 ttgccgcgat ctcgacagtg ccattgctgc cgcaaaaagt ctgctttcag acacattaaa
    25621 atgggtggtg attaccagcg cctccggtaa tgaagaaaat caggagatgc aggtggtggt
    25681 ggtcagtgcc gacagcgtga atgtcatttc ccattcacgg gtaaaaaccg acctgaaagg
    25741 gactggcgac ctgttttgtg ctcagcttat cagtggtttg ctgaaaggga aggcgttaaa
    25801 cgatgcggtg caccgtgcgg ggttgcgcgt actggaagtg atgcgctaca cccagcagca
    25861 tgagagcgat gaattgattt tgccgccgct ggcggaagca taaaaaatgg cgccgatggg
    25921 cgccattttt cactgcggca agaattactt cttgatgcgg ataaccgggg tttcacccac
    25981 ggttacgcta ccggacagtt tgatcagttc tttgatttcg tccatgttgg agataacaac
    26041 cggagtcagg gtagacttgg ctttctcttc cagtagcggc agatcaaatt caatgacagt
    26101 atcgccaact ttcacgcgct gaccttcttc agcaatacgc ttgaagcctt cgcctttcag
    26161 ttcaacggtg tcgataccga agtggacgaa cagttcaacg ccgctatcag attcgataga
    26221 gaacgcgtga ttggtttcaa agattttacc aatggtaccg tctacaggcg cgaccatttt
    26281 gttacccgtt ggtttgatag caataccatc accaacgatt ttttccgcaa aaacgacatc
    26341 cggcacgtct tcgatattga cgatctcgcc agagagcgga gcaatgatct caatagttcc
    26401 ggtatccttc ttgtcgtcgg aaaccagaga tttcagttta tcgaacaaac ccatgatctt
    26461 ctcctaagca gtaaattggg ccgcatctcg tggattagca gattgttttt tcttcaatga
    26521 acttgttaac cagcgtcatt aactcgtccg ttgtcggttg agcaagagcc tgctctgcta
    26581 acaccttcgc atcttcgaag ttcgtgttac ggataatctt cttaatgcgc gggatagaaa
    26641 tggcgctcat agagaattcg tccagaccca tccccagcaa cagaagtgta gcacgttcat
    26701 cgccagcaag ctcaccacac atgccagtcc atttgccttc agcatgagaa gcatcaataa
    26761 cttgcttgat caagttcagc acggacggtg acattggttg gtaaaggtgt gaaatcatat
    26821 cattaccacg gtcaactgcc agagtgtact gcgttaaatc attggtgccg atactaaaga
    26881 aatcaacttc tttggctaaa tgacgtgcaa ttgttgcggc agccggtgtt tccaccatta
    26941 cgccgatttc aattgactcg tcaaacgctt taccttcgtc gcgcagttcc tgtttgtaga
    27001 tttcgatctc tttgcgcagt gcacgcactt cttcaacaga gatgatcatc gggaacataa
    27061 tgcgcaattt accgaaagcc gaggcacgca ggatagcgcg gagctgatcg cgcaggatct
    27121 ctttacgatc catcgcgata cggatagcgc gccagccgag gaacgggttc tcttctttcg
    27181 ggaagttcat gtatggcagc tctttgtcgc cgccgatgtc catggtacga acgataaccg
    27241 cctgcgagcc acacgcttca gccactgctt tgtaagcagc aaactgttct tcttcagtgg
    27301 gcagcgcgtc gcggtccatg aacaggaact cagtacgata cagaccaacg ccttcagcgc
    27361 cgttacgctc tgcaccttca acgtcacgaa ccgtaccaat gttagcgcac acttctacct
    27421 ggtgaccgtc cagcgtaata gctggcagat ctttcagttt agcaagctct gctttttcag
    27481 aagccacttg ctcctgaaca gcgcgcattt tatcaataac ttcgttggtt ggattgacgt
    27541 aaacctgatt atttacggca tccagaatca gatagtcgtc atttttcacc tgagaggtga
    27601 cgctaccggt acccacgata gcaggcagtt ccagagaacg cgccatgata gaggtgtggg
    27661 aagtacggcc acccgcgtcg gtgatgaaac ccagcacctt cttcaggttc agctgtgcgg
    27721 tttcggacgg cgtcaggtca gcggcaacca gaatgacttc atcctgaatg gcgctcaggt
    27781 cgataatctt caggcccagg atgttgcgca gcaggcgctt accgatatca cgtacgtcag
    27841 ccgcacgttc tttcaggtat tcatcatcca gctcttccag ggcagaagcc tgaccttcga
    27901 taacttcatg agcagctgcg tcagctgtca tgtgcttatc tttaatcagg gctatgattt
    27961 cctgctccag ctcctcatct tcgagcagca taatatgccc ttcaaagatg gcttcttttt
    28021 cttcaccgaa cgtttcacca gctttcgttt tgatcgtttc cagctgggct gatgccttgg
    28081 cacgaccgct cagaaaacgt tcaacttcct gatcaacctg gtcggcagaa atttttttcc
    28141 ggtcaatgac aatttcgtct tctttcagaa gcagagcttt accgaaagcg atacccgggg
    28201 atgctaaaat gcctgaaatc ataaccctac cttacttgtg actgattttt aaaagaaccc
    28261 gggaaattac tcgagttccg ccatcagttt aaccagatgt tcaaccgctt tctgctcgtc
    28321 ttcgccttct gcggagatag tcacaacggt accttgagtc aggcccagag tctgcagttt
    28381 aaacaggctt ttcgcgctgg cgcttttgcc gttggaagtc acagtaattt cagaagtgaa
    28441 gcccttagct tcttttacaa actgggcagc agggcgggtg tgcagaccgt tcggagcggt
    28501 aatggtaact tcttgctgga acattgtatt tccccaactt ataggtttag tgttgtggaa
    28561 ctaaagtcta gcctggcggt tcgactttag cctgtattgt tagcgccagt ttttaacaga
    28621 cgcgacgcac gaagagcgca atacagtcca ttgctggcgg gtgttgaccg ctgacgtttc
    28681 gtacgtcatt aaacattatg ccgctaaagg aagaattgaa ccaaatcata aaatcgattc
    28741 agctttggct tttcctgtaa cgattaattt cgcgcatcaa aataattagt ctggttaaat
    28801 accagttccg acaggctgaa tcaatgccag gaggggcgaa agtttgaagc aggccacaaa
    28861 aaagcaccta aaaaggtgct tttttacgca tttttaacaa gctggcatta ctgttgcagt
    28921 tctttctcag tgaagagatc ggcaaacaat gcggtgctta aataacgctc acccgatgac
    28981 ggtagaataa ccacaatatt cttgttggta aagctttcat cttcttgtag tttcaacgcc
    29041 gcagcaacag ctgctccaga agagatacct gcaagaatac cttcttcttc catcagacga
    29101 cgcgcggtag aaatcgcttc ttcattggtg atgccaatga ctttatcgac cagcttgaga
    29161 tcgaggttag ctgggataaa accagcgcca ataccctgaa ttttatgcgg gccaggttta
    29221 atctcttcac cagccagtgc ctgggcgata actggagaat cggttggctc aacggcgaca
    29281 gagataagat cggtcttgcc tttggtgcct ttaatgtagc ggctgacgcc agtcagcgta
    29341 ccgccagtcc caacgccagc aataaataca tcaacctgac cgtcggtatc ttcccagatc
    29401 tccggaccgg tggttttttc gtgaatttca gggtttgccg gattgctgaa ttgttgcagc
    29461 agcaggtatt tctctggatt gctggcgaca atttcttctg ctttttggat tgcgcctttc
    29521 atgcctttag caccttccgt cagcaccagg tttgcaccta acgctttcag cagcttgcgg
    29581 cgttcaatac tcatggtttc tggcatggtc agggtgagtt tgtaaccgcg agcggcagct
    29641 acataggcca gtgcaatccc ggtattaccg ctggtcggtt caaccagttc aacgcctggt
    29701 ttcagcacgc cgcgcttttc ggcatcccaa atcatgttgg caccgatacg gcacttaacg
    29761 ctgaagctgg ggttacgaga ttccaccttc gccagaatgc gtccgttacc gatgcgattc
    29821 aggcgaacca gcggcgtgtg accgatagtc agcgagttat cttcaaaaat cttactcatg
    29881 gcctgtcctt aactgtatga aattgggata caacaggtag catacccgct cagagaatat
    29941 gcggaagtaa ggatttagca tatctatata cagaagggaa ataatgacat gcaagatgga
    30001 ataaggggcg gcataagcca cccctgtttc acacaaacgg tttacaaaat aaccggtaga
    30061 ttgttaccgc cacatcgcgt gtttatcgcg atagcaatcg acccacatcg ccgtcgcgcc
    30121 acaaacggca acgggcatga tgaacagatt aagcagcggg atcatcgtaa acaggctggt
    30181 taaagcacca aactgcatat tggtgatttt gcgtgtgcgc agggcggtgc gcatctcttt
    30241 aaacggcact ttgtggttat cgaaggggta atcgcaatac tggatggcta acatccaggc
    30301 gctaaacagg aaccacagta ccggcgcgac ggtttgccca ataccaggga ttaagtaaag
    30361 aattagcaat acaattgcgc gcggcagata ccaggcaaat ttttgccatt cgcgtttcat
    30421 gattcgcggc acatctttca tgataccgaa aatcccggta tctggcggtg tagcgccagt
    30481 caatcgtgct tccagttgtt cagccaataa accgttaaac ggagcggcaa tccagttagc
    30541 aatcgtggag aagaaatagc caaacactaa cagcacagag atgacagcca gaggccacaa
    30601 cagataactc agccattgta gccagtccgg aacgtaactc atgagagtcg ggatccagac
    30661 atcgagctgt gtaaagagcc accagaatgc gccccccatc aacaaaatat tgaccagcag
    30721 cggtaaaata acgaaacgcc gaatcccagg ttgcgagacg agcttccagc cttgcgcaaa
    30781 atagtaaaaa ccgctgcgtg gggcagatgt gaatgatgaa accataatca ggatgagctc
    30841 cttttgacca atcccaggaa aattctgcgt attttaccgg gtaattgcgc aatggacagt
    30901 taggatatgt tcgaaaaaac agcaaaaagc acgatttcat ctatctttgt gctgtgaaag
    30961 ttaatagtgc acttgcactt gaggtaatcg gcaaatactc ttagtgagta aatgtttgcc
    31021 gtggtggcaa ggtgttagaa caacagagaa tataatgatg caggatttgc gtctgatatt
    31081 aatcattgtt ggcgcgatcg ccataatcgc tttactggta catggtttct ggaccagccg
    31141 taaagaacga tcttctatgt tccgcgatcg gccattaaaa cgaatgaagt caaaacgtga
    31201 cgacgattct tatgacgagg atgtcgaaga tgatgagggc gttggtgagg ttcgtgttca
    31261 ccgcgtgaat catgccccgg ctaacgctca ggagcatgag gctgctcgtc cgtcgccgca
    31321 acaccagtac caaccgcctt atgcgtctgc gcagccgcgt caaccggtcc agcagccgcc
    31381 tgaagcgcag gtaccgccgc aacatgctcc gcgtccagcg cagccggtgc agcagcctgc
    31441 ctatcagccg cagcctgaac agccgttgca gcagccagtt tcgccacagg tcgcgccagc
    31501 gccgcagcct gtgcattcag caccgcaacc ggcacaacag gctttccagc ctgcagaacc
    31561 cgtagcggca ccacagcctg agcctgtagc ggaaccggct ccagttatgg ataaaccgaa
    31621 gcgcaaagaa gcggtgatta tcatgaacgt cgcggcgcat cacggtagcg agctaaacgg
    31681 tgaactgctt cttaacagca ttcaacaagc gggcttcatt tttggcgata tgaatattta
    31741 ccatcgtcat cttagcccgg atggcagcgg cccggcgtta ttcagcctgg cgaatatggt
    31801 gaaaccggga acctttgatc ctgaaatgaa ggatttcact actccgggtg tcaccatctt
    31861 tatgcaggta ccgtcttacg gtgacgagct gcagaacttc aagctgatgc tgcaatctgc
    31921 gcagcatatt gccgatgaag tgggcggtgt cgtgcttgac gatcagcgcc gtatgatgac
    31981 tccgcagaaa ttgcgcgagt accaggacat catccgcgaa gtcaaagacg ccaacgcctg
    32041 atacacttaa ggcaaattaa ctcctcttcg aacccccgct tgtcgggggt ttttagcatt
    32101 gatggtgcga tatggaatca atcgaacaac aactgacaga actgcgaacg acgcttcgcc
    32161 atcatgaata tctttatcat gtgatggatg cgccggaaat tcccgacgct gaatacgaca
    32221 ggctgatgcg cgaactgcgc gagctggaaa ccaaacatcc agaactgatt acgcctgatt
    32281 cgcctaccca acgtgtaggc gctgcgccgc tggcggcttt cagccagata cgccatgaag
    32341 taccaatgct gtcactggat aacgtttttg atgaagaaag ctttcttgct ttcaacaaac
    32401 gtgtgcagga ccgtctgaaa aacaacgaga aagtcacctg gtgctgtgag ctgaagctgg
    32461 atggccttgc cgtcagtatt ctgtatgaaa atggcgtttt agtcagtgcc gcgacccgtg
    32521 gcgatggcac caccggggaa gatatcacgt ctaatgtgcg tactattcgc gctattccgc
    32581 tgaagctgca cggagagaat atcccggcgc gtctggaagt gcgtggtgaa gtgttcctgc
    32641 cgcaggcggg gttcgaaaag attaacgaag atgcgcgacg cacgggcggg aaagtttttg
    32701 ctaacccacg taatgcggca gctggttcac tgcgtcagct tgatccgcgt attacagcga
    32761 agcgaccgct cacttttttc tgctatggcg ttggtgttct ggaaggtggc gagctgccgg
    32821 atactcatct tggccgttta ctgcaattta aaaagtgggg gttgccggtc agcgatcggg
    32881 taacgctttg tgaatcggcg gaagaagtgc tggcgttcta tcacaaagtg gaagaagacc
    32941 gcccgacgct gggctttgat atcgacggcg tggtgattaa ggtcaactca ctggaacagc
    33001 aggagcagct tggctttgtc gcgcgtgccc cgcgctgggc ggtagcgttt aaattcccgg
    33061 cgcaggagca gatgaccttt gtgcgtgacg tcgagtttca ggttgggcgt actggcgcga
    33121 ttacgcctgt tgcgcgtctg gaacctgtcc atgttgctgg cgtgctggtg agtaacgcaa
    33181 ccttacacaa tgcggatgaa atcgaacgtc ttggtttacg cattggcgat aaagtggtga
    33241 ttcgccgcgc tggcgacgtg atcccgcagg tggttaacgt cgtgctttct gaacgcccgg
    33301 aagatacccg tgaggttgta ttcccgacgc attgtccggt atgtggttct gacgttgagc
    33361 gtgtggaagg tgaagcggtt gcccgctgta ccggtggcct gatttgcggt gcgcagcgta
    33421 aagagtcgct gaaacacttt gtttcccgcc gtgcgatgga tgttgacgga atgggcgaca
    33481 aaatcatcga tcagctggtt gaaaaagaat atgtccatac tccggcagat ctgttcaaac
    33541 tcaccgcagg caaactgacc ggactggagc gtatggggcc aaaatcggca caaaacgtgg
    33601 ttaacgcgct ggaaaaagcg aaagaaacca cctttgctcg cttcctctat gcacttggca
    33661 tccgtgaagt cggcgaggcc accgcagcag gtctggcggc atatttcggc acgctggaag
    33721 cgctggaagc cgcttcgatt gaagagctgc aaaaggtgcc tgatgttggc attgtcgttg
    33781 catcccacgt tcacaacttc tttgccgaag aaagcaaccg caatgtcatc agcgagctgt
    33841 tggcggaagg tgttcactgg cctgcgccga tcgttatcaa cgcggaagag attgacagcc
    33901 cgtttgctgg taaaaccgtg gtgcttacgg gcagcttaag ccagatgtcg cgtgatgacg
    33961 ctaaagctcg actggttgaa ctgggcgcga aagtcgcggg cagcgtgtcg aagaaaaccg
    34021 atctggtgat tgccggagaa gcggcaggtt ctaaactggc gaaagcgcag gaactgggca
    34081 ttgaagtcat cgacgaagcg gaaatgctgc gtttgctggg tagctgagat ggaaaaagag
    34141 cagctgattg aaatagccaa tacgataatg ccgtttggca aatataaagg gcgtcgctta
    34201 atcgacctgc cggaggagta tctgctgtgg tttgcccgca aagatgaatt cccggcaggg
    34261 aagctcggtg agctaatgca aatcacgctg ctgattaaaa ccgaggggct gacgcaactg
    34321 gtccagcccc tgaagcgtcc gctttaagct ttatcggcgc tgctttcctg ctgtgcctgt
    34381 aactgttcgg tctggcgttt gtatcgacgc gccagcaccg cacagaccat caattggatc
    34441 tgatggaaaa tcattagggg cagcaccatc ataccgatca ccgatgtggg gaacagaatg
    34501 tttgccatcg ggatgccatt tgccagactc tttttcgaac cacaaaagac gatagtaatt
    34561 tcatctgcct tattgaagcc cagtcggcgt gccataaaga cgttaactac aatcacgata
    34621 gccagaagaa cgcagctgac caccacgata aacagcaatg atccccagcc aactttatgc
    34681 cagataccat taacgacggc ttcgctgaat gctgtataaa ccaccaacag aatggacgtc
    34741 tggtcagttt tcgcaatcca ttttttattg cgcgacaccc agtcaccaat ccacggccgg
    34801 gaaagatgcc ccaacacaaa cggcagcagc agttgcagca taattttacc gacctgctca
    34861 aggctgcccc ctgcaccgtg aacattcatc accagaccaa ccagcaatgg tgaaaggaaa
    34921 atccccagca ggctggatgc tgacgcagaa caaaccgccg ccgcgacgtt accgcccgcc
    34981 attgacgtga aggcgattgc agactgcacg gtagccggga gaatgcacaa gtagagaaaa
    35041 ccggagtaga gcatcgggtc gacatttacc ggtttccacc aggcaaacag tacacccaga
    35101 atcggaaaca gcacgaaggt gctgcaaatt acccacaaat gcagtcgcca gtgaccaccg
    35161 ccagcaataa tcgcctcacg cgacaacttc gcgccgtgca taaagaacag cagggcaatc
    35221 gctgcggtgg tcagattttc aaagaagggg acgaaatcgc ctctggccgg aaagaaagag
    35281 gccagcaaca ccaccgtgat cagggttaag gtgaaaggat cgaggatacg aaaaagtttc
    35341 ataaagactc cagatgatcg atggttctat tgtgcttctt ccagtttgag aaataaaatt
    35401 gatttattgc atccattcat gaagaaaaca gatgaattat tctttaaaac aattaaaggt
    35461 tttcgtcaca gtagcgcagg agaaaagttt tagtcgtgca ggagagcgta tcggcctgag
    35521 ccagtcggca gtgagtcaca gtgtgaagga actggaaaat catactggtg ttcgcctgct
    35581 ggacagaacc acgcgtgaag tggtgcttac agatgcaggg cagcagctgg ctttgcgtct
    35641 tgagcgactg ctggatgaac tgaacagcac gttgcgcgat accgggcgta tggggcaaca
    35701 actgagcgga aaagttcggg tcgctgccag ccagaccatt tccgcgcatc ttattccgca
    35761 atgcattgct gaaagccatc gccgctatcc agatattcag tttgtcctgc acgatcgtcc
    35821 gcagcagtgg gtgatggaaa gtattcgcca gggagatgtc gactttggca tcgtcatcga
    35881 tccaggccca gtgggcgatc tgcaatgtga agcgattctt tccgagcctt tctttctgct
    35941 ttgccatcgc gatagcgctt tggccgtcga agattatgta ccctggcagg cattacaggg
    36001 agcgaaattg gtgttgcagg attacgcgtc aggcagccga ccgctgattg acgcagcgct
    36061 ggcgcgcaat ggtattcagg cgaatattgt gcaggagatt ggacatccgg cgacgctgtt
    36121 cccgatggta gccgcaggca tcggcatcag tattcttccc gcactggcgc taccattgcc
    36181 tgaaggtagc ccactggtgg taaagcgcat tacgccggtt gttgaacgcc agttgatgct
    36241 ggtgcgcagg aagaaccgtt cactttccac cgccgctgaa gcgttatggg acgtggtacg
    36301 agaccagggc aatgcgctaa tggctggtcg ggaaggggat ccgttgtatc agatatagag
    36361 tgaattcata ctgccgggag ccccggcagt aatcaatcag aagttaaatt tgatggagta
    36421 gattatcgca tcctggtaaa aatcttcgcc cagtttgtca tccgcatatc gatactgaat
    36481 ccccgtagta atgtgtgatg ttgcattcca ccacagtgca actgcgccat tcagcccttc
    36541 tttaccgcca ttacccgctg cataggtagc gtcacggtca aactcgtact cgttccagtt
    36601 ggtaagagtg aatttctcgc tgcccagcat aaagttgtaa cctgcaaccc agccggcgac
    36661 atagccgttg tcgccagtgt aataggtttg atctgtataa cgcttagcaa agaacggttt
    36721 gaaccaccaa ccgctgccgg tgaaattgta gccgataccg tacaggaaca tatcgtcgtg
    36781 gaaattcacg cgattcgcag aaccgtaggt gccatacgca tgcaagtaga gattaaatcc
    36841 ggtgtcaccc aggtaaatac ggttggtatt tttaaaggta taacgctgct cgctacctgg
    36901 tttattatgg cgaccgttat agaagttttc ccagtcgaag aacccgtaca tttctcccca
    36961 actaaagtta gcgccacctt caaattcaag ataaccaaaa tcatctttgt gtgatttggt
    37021 cgacgatttt tcggtagtcc ggctggtcca gtccagataa tgcacgccga tatctgcaaa
    37081 accgccttta aattctgcat gagatacaac aggtatcgct aatatagagg aaagtgtcag
    37141 agttaaaaga tgttttttca tagcataatt ccctatgccg atcgttatta gtagattgct
    37201 taataaagtg gtttgctaaa aacgacatat tgttaatgca aaggaattaa tatcgccaat
    37261 acagttttta ccgtggatta atagagaaaa gatataaatc catcgatatc gaatcgctat
    37321 tgaatgcgtg agaaaagtgt attggtaaga gccgggacaa cctcgctata aaaaggcgtt
    37381 agattccacc ctacagaaaa aggatatgta tgtctcaggt tcaattttct cataacccgc
    37441 tgttttgcat agatattatc aagacttata aacctgattt cacgccacga gtggccttta
    37501 ttttaggttc cgggctcggc gcgctggccg atcagattga gaacgctgtc gcaatttcct
    37561 acgaaaagct gccgggcttc ccggtaagta cggtacatgg tcatgcgggg gagttagtgc
    37621 tgggccatct ccagggcgta ccggtggtat gtatgaaagg tcgcggacat ttctacgaag
    37681 gtcgtggaat gaccattatg actgacgcaa ttcgtacctt taagctgctg ggctgcgagc
    37741 tactgttctg caccaatgcg gcaggctcgc tgcgtccgga agtgggggca ggcagcctgg
    37801 tcgcattgaa agatcatatc aacaccatgc ccggtacgcc gatggtgggt cttaacgatg
    37861 atcgttttgg agagcgcttc ttctcactgg caaatgccta cgatgcggaa taccgcgcac
    37921 tgttacaaaa agtggcgaaa gaagagggtt tccctctgac ggagggcgtg ttcgtctcgt
    37981 atccggggcc gaatttcgag actgcggcgg aaattcgcat gatgcaaatt attggtgggg
    38041 atgttgttgg tatgtctgtg gtgcctgagg ttatttcagc tcgccattgc gaccttaaag
    38101 tcgttgcggt ctctgcgatt accaatatgg cggaaggttt aagcgatgtg aaattgtcac
    38161 atgcgcaaac gctggcggct gcggagctct ctaagcagaa cttcatcaac cttatttgtg
    38221 gctttctgcg caaaattgcc tgagaaaaac aataagaaac ggcctcgcgg gtgaggccaa
    38281 atgatgcgac aaggaaacga ttatgagcat cgcgatgcgc ttaaaggtaa tgtccttttt
    38341 gcaatatttt atctggggga gctggctggt taccctcggc tcttacatga ttaataccct
    38401 tcatttcacc ggcgctaatg ttggcatggt ttacagttcc aaagggatcg ccgcgattat
    38461 tatgcctggt ataatgggga tcatcgcaga caaatggctg cgcgcagaac gtgcatacat
    38521 gctgtgtcac ctggtgtgtg cgggcgtact tttttatgcg gcatccgtaa ctgatccgga
    38581 tatgatgttt tgggtgatgt tagtcaatgc gatggcgttt atgccgacta ttgcgttatc
    38641 gaacagcgtc tcttattcct gtcttgccca ggcagggctt gacccggtga ccgctttccc
    38701 gcccattcgc gtttttggta cggtggggtt cattgtcgcg atgtgggcag taagcctgct
    38761 gcatctggaa ttgagtagtc tgcagctgta tatcgcgtcc ggtgcgtcat tgctgctgtc
    38821 ggcttatgcg ctgactttgc cgaagattcc ggttgcggag aaaaaagcga ccacatcgct
    38881 tgccagcaag ctgggtctgg atgccttcgt gctgtttaaa aatccacgca tggccatctt
    38941 tttcctcttt gccatgatgc tgggtgcggt actgcaaatt accaacgttt ttggtaatcc
    39001 gttcctacat gatttcgccc gtaacccgga gtttgctgac agttttgtgg tgaaatatcc
    39061 ctccatttta ctgtcagttt cacagatggc agaagtgggc tttatactga ctatcccatt
    39121 ctttttaaag cgatttggca ttaaaaccgt catgctgatg agtatggtgg cctggacgct
    39181 gcgctttggc ttcttcgcct atggcgatcc gtcaacaacc ggatttattt tgctgctgct
    39241 gtcgatgatt gtttatggct gtgcattcga tttcttcaat atttctggtt cggtatttgt
    39301 cgaacaggaa gttgattcca gcattcgtgc cagcgcgcag gggctcttta tgaccatggt
    39361 aaatggtgtc ggcgcatggg ttggctcgat tctgagtggc atggcagtag attacttttc
    39421 ggtggatggc gtaaaagact ggcaaactat ctggctggtg tttgcaggat atgctctttt
    39481 tctcgcagtg atatttttct ttgggtttaa atataatcat gaccctgaaa agataaagca
    39541 tcgagcggtg gctcattaaa aggattcgca gctctgctct tcagagctgc ttttatgata
    39601 aaggttaatt agtgaaagat attttatttt cttccggcgt tggttttggc attggtgctt
    39661 tg
//
LOCUS       DAZKHF020000016        35869 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2201S31241
            SAMN43267800-rid25106413.denovo.016, whole genome shotgun sequence.
ACCESSION   DAZKHF020000016 DAZKHF020000000
VERSION     DAZKHF020000016.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267800
            Sequence Read Archive: SRR30307263
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 35869)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 35869)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 35869)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350581.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350581.2
            Genome Coverage       :: 151x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:21:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,652
            CDSs (total)                      :: 5,557
            Genes (coding)                    :: 5,320
            CDSs (with protein)               :: 5,320
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 237
            CDSs (without protein)            :: 237
            Pseudo Genes (ambiguous residues) :: 0 of 237
            Pseudo Genes (frameshifted)       :: 102 of 237
            Pseudo Genes (incomplete)         :: 130 of 237
            Pseudo Genes (internal stop)      :: 52 of 237
            Pseudo Genes (multiple problems)  :: 41 of 237
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..35869
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267800-rid25106413.denovo.016"
                     /serotype="O 26:H11"
                     /isolate="2201S31241"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-01"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            complement(106..837)
                     /gene="rlmB"
                     /locus_tag="ACHOSC_RS03425"
                     /old_locus_tag="pgaptmp_000685"
     CDS             complement(106..837)
                     /gene="rlmB"
                     /locus_tag="ACHOSC_RS03425"
                     /old_locus_tag="pgaptmp_000685"
                     /EC_number="2.1.1.185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (guanosine(2251)-2'-O)-methyltransferase RlmB"
                     /protein_id="HGU4546681.1"
                     /translation="MSEMIYGIHAVQALLERAPERFQEVFILKGREDKRLLPLIHALE
                     SQGVVIQLANRQYLDEKSDGAVHQGIIARVKPGRQYQENDLPDLIASLDQPFLLILDG
                     VTDPHNLGACLRSADAAGVHAVIVPKDRSAQLNATAKKVACGAAESVPLIRVTNLART
                     MRMLQEENIWIVGTAGEADHTLYQSKMTGRLALVMGAEGEGMRRLTREHCDELISIPM
                     AGSVSSLNVSVATGICLFEAVRQRS"
     gene            complement(1017..3458)
                     /gene="rnr"
                     /locus_tag="ACHOSC_RS03430"
                     /old_locus_tag="pgaptmp_000686"
     CDS             complement(1017..3458)
                     /gene="rnr"
                     /locus_tag="ACHOSC_RS03430"
                     /old_locus_tag="pgaptmp_000686"
                     /EC_number="3.1.13.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418600.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004540 - RNA nuclease activity [Evidence
                     IEA];
                     GO_process: GO:0016070 - RNA metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease R"
                     /protein_id="HGU4546682.1"
                     /translation="MSQDPFQEREAEKYANPIPSREFILEHLTKREKPASRDELAVEL
                     HIEGEEQLEGLRRRLRAMERDGQLVFTRRQCYALPERLDLVKGTVIGHRDGYGFLRVE
                     GRKDDLYLSSEQMKTCIHGDQVLAQPLGADRKGRREARIVRVLVPKTSQIVGRYFTEA
                     GVGFVVPDDSRLSFDILIPPDQIMGARMGFVVVVELTQRPTRRTKAVGKIVEVLGDNM
                     GTGMAVDIALRTHEIPYIWPQAVEQQVAGLKEEVPEEAKAGRVDLRDLPLVTIDGEDA
                     RDFDDAVYCEKKRGGGWRLWVAIADVSYYVRPPTPLDREARNRGTSVYFPSQVIPMLP
                     EVLSNGLCSLNPQVDRLCMVCEMTVSSKGRLTGYKFYEAVMSSHARLTYTKVWHILQG
                     DQDLREQYAPLVKHLEELHNLYKVLDKAREERGGISFESEEAKFIFNAERRIERIEQT
                     QRNDAHKLIEECMILANISAARFVEKAKEPALFRIHDKPSTEAITSFRSVLAELGLEL
                     PGGNKPEPRDYAELLESVADRPDAEMLQTMLLRSMKQAIYDPENRGHFGLALQSYAHF
                     TSPIRRYPDLTLHRAIKYLLAKEQGHQGNTTETGGYHYSMEEMLQLGQHCSMAERRAD
                     EATRDVADWLKCDFMLDQVGNVFKGVISSVTGFGFFVRLDDLFIDGLVHVSSLDNDYY
                     RFDQVGQRLMGESSGQTYRLGDRVEVRVEAVNMDERKIDFSLISSERAPRNVGKTARE
                     KAKKGDAGKKGGKRRQVGKKVNFEPDSAFRGEKKTKPKAAKKDARKAKKPSAKTQKIA
                     AATKAKRAAKKKVAE"
     gene            complement(3497..3922)
                     /gene="nsrR"
                     /locus_tag="ACHOSC_RS03435"
                     /old_locus_tag="pgaptmp_000687"
     CDS             complement(3497..3922)
                     /gene="nsrR"
                     /locus_tag="ACHOSC_RS03435"
                     /old_locus_tag="pgaptmp_000687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005134424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitric oxide-sensing transcriptional repressor
                     NsrR"
                     /protein_id="HGU4546683.1"
                     /translation="MQLTSFTDYGLRALIYMASLPEGRMTSISEVTDVYGVSRNHMVK
                     IINQLSRAGYVTAVRGKNGGIRLGKPASAIRIGDVVRELEPLSLVNCSSEFCHITPAC
                     RLKQALSKAVQSFLTELDNYTLADLVEENQPLYKLLLVE"
     gene            complement(4127..5425)
                     /gene="purA"
                     /locus_tag="ACHOSC_RS03440"
                     /old_locus_tag="pgaptmp_000688"
     CDS             complement(4127..5425)
                     /gene="purA"
                     /locus_tag="ACHOSC_RS03440"
                     /old_locus_tag="pgaptmp_000688"
                     /EC_number="6.3.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019842885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004019 - adenylosuccinate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate synthase"
                     /protein_id="HGU4546684.1"
                     /translation="MGNNVVVLGTQWGDEGKGKIVDLLTERAKYVVRYQGGHNAGHTL
                     VINGEKTVLHLIPSGILRENVTSIIGNGVVLSPAALMKEMKELEDRGIPVRERLLLSE
                     ACPLILDYHVALDNAREKARGAKAIGTTGRGIGPAYEDKVARRGLRVGDLFDKETFAE
                     KLKEVMEYHNFQLVNYYKAEAVDYQKVLDDTMAVADILTSMVVDVSDLLDQARQRGDF
                     VMFEGAQGTLLDIDHGTYPYVTSSNTTAGGVATGSGLGPRYVDYVLGILKAYSTRVGA
                     GPFPTELFDETGEFLCKQGNEFGATTGRRRRTGWLDTVAVRRAVQLNSLSGFCLTKLD
                     VLDGLKEVKLCVAYRMPDGREVTTTPLAADDWKGVEPIYETMPGWSESTFGVKDRSGL
                     PQAALNYIKRIEELTGVPIDIISTGPDRTETMILRDPFDA"
     gene            complement(5529..5726)
                     /gene="yjeT"
                     /locus_tag="ACHOSC_RS03445"
                     /old_locus_tag="pgaptmp_000689"
     CDS             complement(5529..5726)
                     /gene="yjeT"
                     /locus_tag="ACHOSC_RS03445"
                     /old_locus_tag="pgaptmp_000689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2065 domain-containing protein"
                     /protein_id="HGU4546685.1"
                     /translation="MNSTIWLALALVLVLEGLGPMLYPKAWKKMISAMTNLPDNILRR
                     FGGGLVVAGVVVYYMLRKTIG"
     gene            complement(5808..6812)
                     /gene="hflC"
                     /locus_tag="ACHOSC_RS03450"
                     /old_locus_tag="pgaptmp_000690"
     CDS             complement(5808..6812)
                     /gene="hflC"
                     /locus_tag="ACHOSC_RS03450"
                     /old_locus_tag="pgaptmp_000690"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protease modulator HflC"
                     /protein_id="HGU4546686.1"
                     /translation="MRKSVIAIIIIVLVVLYMSVFVVKEGERGITLRFGKVLRDDDNK
                     PLVYEPGLHFKIPFIETVKMLDARIQTMDNQADRFVTKEKKDLIVDSYIKWRISDFSR
                     YYLATGGGDISQAEVLLKRKFSDRLRSEIGRLDVKDIVTDSRGRLTLEVRDALNSGSA
                     GTEDEVTTPAADNAIAEAAERVTAETKGKVPVINPNSMAALGIEVVDVRIKQINLPTE
                     VSEAIYNRMRAEREAVARRHRSQGQEEAEKLRATADYEVTRTLAEAERQGRIMRGEGD
                     AEAAKLFADAFSKDPDFYAFIRSLRAYENSFSGNQDVMVMSPDSDFFRYMKTPTSATR
                     "
     gene            complement(6815..8074)
                     /gene="hflK"
                     /locus_tag="ACHOSC_RS03455"
                     /old_locus_tag="pgaptmp_000691"
     CDS             complement(6815..8074)
                     /gene="hflK"
                     /locus_tag="ACHOSC_RS03455"
                     /old_locus_tag="pgaptmp_000691"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsH protease activity modulator HflK"
                     /protein_id="HGU4546687.1"
                     /translation="MAWNQPGNNGQDRDPWGSSKPGGNSEGNGNKGGRDQGPPDLDDI
                     FRKLSKKLGGLGGGKGTGSGGGSSSQGPRPQLGGRVVTIAAAAIVIIWAASGFYTIKE
                     AERGVVTRFGKFSHLVEPGLNWKPTFIDEVKPVNVEAVRELAASGVMLTSDENVVRVE
                     MNVQYRVTNPEKYLYSVTSPDDSLRQATDSALRGVIGKYTMDRILTEGRTVIRSDTQR
                     ELEETIRPYDMGITLLDVNFQAARPPEEVKAAFDDAIAARENEQQYIREAEAYTNEVQ
                     PRANGQAQRILEEARAYKAQTILEAQGEVARFAKLLPEYKAAPEITRERLYIETMEKV
                     LGNTRKVLVNDKGGNLMVLPLDQMLKGGNAPAAKSDNGASNLLRLPPASSSTTSGASN
                     TSSTSQGDIMDQRRANAQRNDYQRQGE"
     gene            complement(8160..9440)
                     /gene="hflX"
                     /locus_tag="ACHOSC_RS03460"
                     /old_locus_tag="pgaptmp_000692"
     CDS             complement(8160..9440)
                     /gene="hflX"
                     /locus_tag="ACHOSC_RS03460"
                     /old_locus_tag="pgaptmp_000692"
                     /EC_number="3.6.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome rescue GTPase HflX"
                     /protein_id="HGU4546688.1"
                     /translation="MFDRYDAGEQAVLVHIYFTQDKDMEDLQEFESLVSSAGVEALQV
                     ITGSRKAPHPKYFVGEGKAVEIAEAVKATGASVVLFDHALSPAQERNLERLCECRVID
                     RTGLILDIFAQRARTHEGKLQVELAQLRHLATRLVRGWTHLERQKGGIGLRGPGETQL
                     ETDRRLLRNRIVQIQSRLERVEKQREQGRQSRIKADVPTVSLVGYTNAGKSTLFNRIT
                     EARVYAADQLFATLDPTLRRIDVADVGETVLADTVGFIRHLPHDLVAAFKATLQETRQ
                     ATLLLHVIDAADVRVQENIEAVNTVLEEIDAHEIPTLLVMNKIDMLEDFEPRIDRDEE
                     NKPIRVWLSAQTGAGIPQLFQALTGRLSGEVAQHTLRLPPQEGRLRSRFYQLQAIEKE
                     WMEEDGSVSLQVRMPIVDWRRLCKQEPALIDYLI"
     gene            complement(9517..9825)
                     /gene="hfq"
                     /locus_tag="ACHOSC_RS03465"
                     /old_locus_tag="pgaptmp_000693"
     CDS             complement(9517..9825)
                     /gene="hfq"
                     /locus_tag="ACHOSC_RS03465"
                     /old_locus_tag="pgaptmp_000693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA chaperone Hfq"
                     /protein_id="HGU4546689.1"
                     /translation="MAKGQSLQDPFLNALRRERVPVSIYLVNGIKLQGQIESFDQFVI
                     LLKNTVSQMVYKHAISTVVPSRPVSHHSNNAGGGTSSNYHHGSSAQNTSAQQDSEETE
                     "
     gene            complement(9911..10861)
                     /gene="miaA"
                     /locus_tag="ACHOSC_RS03470"
                     /old_locus_tag="pgaptmp_000694"
     CDS             complement(9911..10861)
                     /gene="miaA"
                     /locus_tag="ACHOSC_RS03470"
                     /old_locus_tag="pgaptmp_000694"
                     /EC_number="2.5.1.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0052381 - tRNA dimethylallyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (adenosine(37)-N6)-dimethylallyltransferase
                     MiaA"
                     /protein_id="HGU4546690.1"
                     /translation="MSDISKASLPKAIFLMGPTASGKTALAIELRKILPVELISVDSA
                     LIYKGMDIGTAKPNAEELLAAPHRLLDIRDPSQAYSAADFRRDALAEMADITAAGRIP
                     LLVGGTMLYFKALLEGLSPLPSADPEVRARIEQQAVEQGWESLHRQLQEVDPVAAARI
                     HPNDPQRLSRALEVFFISGKTLTELTQTSGDALPYQVHQFAIAPASRELLHQRIEQRF
                     HQMLASGFEAEVRALFARGDLHTDLPSIRCVGYRQMWSYLEGEISYDEMVYRGVCATR
                     QLAKRQITWLRGWEGVHWLDSEKPEQARDEVLQVVGAIAG"
     gene            complement(10854..12701)
                     /gene="mutL"
                     /locus_tag="ACHOSC_RS03475"
                     /old_locus_tag="pgaptmp_000695"
     CDS             complement(10854..12701)
                     /gene="mutL"
                     /locus_tag="ACHOSC_RS03475"
                     /old_locus_tag="pgaptmp_000695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710035.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0032300 - mismatch repair complex
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0030983 - mismatched DNA binding [Evidence
                     IEA];
                     GO_process: GO:0006298 - mismatch repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair endonuclease MutL"
                     /protein_id="HGU4546691.1"
                     /translation="MPIQVLPPQLANQIAAGEVVERPASVVKELVENSLDAGATRIDI
                     DIERGGAKLIRIRDNGCGIKKDELALALARHATSKIASLDDLEAIISLGFRGEALASI
                     SSVSRLTLTSRTAEQQEAWQAYAEGRDMNVTVKPAAHPVGTTLEVLDLFYNTPARRKF
                     LRTEKTEFSHIDEIIRRIALARFDVTINLSHNGKIVRQYRAVPEGGQKERRLGAICGT
                     AFLEQALAIEWQHGDLTLRGWVADPNHTTPALAEIQYCYVNGRMMRDRLINHAIRQAC
                     EDKLGADQQPAFVLYLEIDPHQVDVNVHPAKHEVRFHQSRLVHDFIYQGVLSVLQQQL
                     ETPLPLDDEPQPAPRPIPENRVAAGRNHFAEPAAREPVAPRYSPAPASGSRPAAPWPN
                     AQPGYQKQQGEVYRQLLQTPAPMQKPKAPEPQEPALAANSQSFGRVLTIVHSDCALLE
                     RDGNISLLALPVAERWLRQAQLTPGEAPVCAQPLLIPLRLKVSGEEKSALEKAQSALA
                     ELGIDFQSDAQHVTIRAVPLPLRQQNLQILIPELIGYLAKQSVFEPGNIAQWIARNLM
                     SEHAQWSMAQAITLLADVERLCPQLVKTPPGGLLQSVDLHPAIKALKDE"
     gene            complement(12711..14051)
                     /gene="amiB"
                     /locus_tag="ACHOSC_RS03480"
                     /old_locus_tag="pgaptmp_000696"
     CDS             complement(12711..14051)
                     /gene="amiB"
                     /locus_tag="ACHOSC_RS03480"
                     /old_locus_tag="pgaptmp_000696"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008745 - N-acetylmuramoyl-L-alanine
                     amidase activity [Evidence IEA];
                     GO_process: GO:0009253 - peptidoglycan catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase AmiB"
                     /protein_id="HGU4546692.1"
                     /translation="MMYRIRNWLVATLLLLLCTPVGAATLSDIQVSNGNQQARITLSF
                     IGDPDYAFSHQSKRTVALDIKQTGVIQGLPLLFSGNNLVKAIRSGTPKDAQTLRLVVD
                     LTENGKTEAVKRQNGSNYTVVFTINADAPPPPPPPPVVAKRVETPAVVAPRVSEPARN
                     PFKTESNRTTGVISSNTVTRPAARATANTGDKIIIAIDAGHGGQDPGAIGPGGTREKN
                     VTIAIARKLRTLLNDDPMFKGVLTRDGDYFISVMGRSDVARKQNANFLVSIHADAAPN
                     RSATGASVWVLSNRRANSEMASWLEQHEKQSELLGGAGDVLANSQSDPYLSQAVLDLQ
                     FGHSQRVGYDVATSMISQLQRIGEIHKRRPEHASLGVLRSPDIPSVLVETGFISNNSE
                     ERLLASDDYQQQLAEAIYKGLRNYFLAHPMQSAPQGATAQTASTVTTPDRTLPN"
     gene            complement(14070..14531)
                     /gene="tsaE"
                     /locus_tag="ACHOSC_RS03485"
                     /old_locus_tag="pgaptmp_000697"
     CDS             complement(14070..14531)
                     /gene="tsaE"
                     /locus_tag="ACHOSC_RS03485"
                     /old_locus_tag="pgaptmp_000697"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0002949 - tRNA threonylcarbamoyladenosine
                     modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     ATPase subunit type 1 TsaE"
                     /protein_id="HGU4546693.1"
                     /translation="MMNRVIPLPDEQATLDLGERVAKACDGATVIYLYGDLGAGKTTF
                     SRGFLQALGHQGNVKSPTYTLVEPYTLDNLMVYHFDLYRLADPEELEFMGIRDYFAND
                     AICLVEWPQQGTGVLPDPDVEIHIDYQAQGREARVSAVSSAGELLLARLAG"
     gene            complement(14503..16050)
                     /gene="nnr"
                     /locus_tag="ACHOSC_RS03490"
                     /old_locus_tag="pgaptmp_000698"
     CDS             complement(14503..16050)
                     /gene="nnr"
                     /locus_tag="ACHOSC_RS03490"
                     /old_locus_tag="pgaptmp_000698"
                     /EC_number="4.2.1.136"
                     /EC_number="5.1.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052855 - ADP-dependent NAD(P)H-hydrate
                     dehydratase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional ADP-dependent NAD(P)H-hydrate
                     dehydratase/NAD(P)H-hydrate epimerase"
                     /protein_id="HGU4546694.1"
                     /translation="MTDHTMKKNPVSIPHTVWHADDIRRGEREAADALGLTLYELMLR
                     AGEAAFQVCRSAYPDARHWLVLCGHGNNGGDGYVVARLATAVGIEVTLLAQESDKPLP
                     EEAALAREAWLNAGGEIHASNIVWPESVDLIVDALLGTGLQQAPRESISQLIDHANSH
                     PAPIAAVDIPSGLLAETGATPGAVINADHTITFIALKPGLLTGKARDVTGQLHFDSLG
                     LDSWLAGQETKIQRFSAEQLSHWLKPRRPTSHKGDHGRLVIIGGDHGTAGAIRMTGEA
                     ALRAGAGLVRVLTRSENIAPLLTARPELMVHELTMDSLTESLEWADVVVIGPGLGQQE
                     WGKKALQKVENFRKPMLWDADALNLLAINPDKRHNRVITPHPGEAARLLGCSVAEIES
                     DRLHCAKRLVQRYGGVAVLKGAGTVVAAHPDALGIIDAGNAGMASGGMGDVLSGIIGA
                     LLGQKLSPYDAACAGCVAHGAAADVLAARFGTRGMLATDLFSTLQRIVNPEVTDKNHD
                     ESSNSAP"
     gene            16049..17188
                     /gene="queG"
                     /locus_tag="ACHOSC_RS03495"
                     /old_locus_tag="pgaptmp_000699"
     CDS             16049..17188
                     /gene="queG"
                     /locus_tag="ACHOSC_RS03495"
                     /old_locus_tag="pgaptmp_000699"
                     /EC_number="1.17.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313170.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0052693 - epoxyqueuosine reductase
                     activity [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA];
                     GO_process: GO:0008616 - queuosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA epoxyqueuosine(34) reductase QueG"
                     /protein_id="HGU4546695.1"
                     /translation="MSEPLDLNQLAQKIKQWGLELGFQQVGITDTDLSESEPKLQAWL
                     DKQYHGEMDWMARHGMLRARPHELLPGTLRVISVRMNYLPANAAFASTLKNPKLGYVS
                     RYALGRDYHKLLRNRLKKLGEMIQQHCVSLNFRPFVDSAPILERPLAAKAGLGWTGKH
                     SLILNREAGSFFFLGELLVDIPLPVDQPVEEGCGKCIACMTICPTGAIVEPYTVDARR
                     CISYLTIELEGAIPEELRPLMGNRIYGCDDCQLICPWNRYSQLTTEDDFSPRKPLHAP
                     ELIELFAWSEEKFLKVTEGSAIRRIGHLRWLRNIAVALGNAPWDETILAALESRKGEH
                     PLLDEHIAWAMAQQIERRNACIVEVQLPKKQRLVRVIEKGLPRDA"
     gene            complement(17171..17224)
                     /locus_tag="ACHOSC_RS03500"
                     /old_locus_tag="pgaptmp_000700"
     CDS             complement(17171..17224)
                     /locus_tag="ACHOSC_RS03500"
                     /old_locus_tag="pgaptmp_000700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4546696.1"
                     /translation="MRFYFYSQAVDESGVTW"
     gene            complement(17458..17533)
                     /locus_tag="ACHOSC_RS03505"
                     /old_locus_tag="pgaptmp_000701"
     tRNA            complement(17458..17533)
                     /locus_tag="ACHOSC_RS03505"
                     /old_locus_tag="pgaptmp_000701"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(17498..17500),aa:Gly,seq:gcc)
     gene            complement(17569..17644)
                     /locus_tag="ACHOSC_RS03510"
                     /old_locus_tag="pgaptmp_000702"
     tRNA            complement(17569..17644)
                     /locus_tag="ACHOSC_RS03510"
                     /old_locus_tag="pgaptmp_000702"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(17609..17611),aa:Gly,seq:gcc)
     gene            complement(17802..17877)
                     /locus_tag="ACHOSC_RS03515"
                     /old_locus_tag="pgaptmp_000703"
     tRNA            complement(17802..17877)
                     /locus_tag="ACHOSC_RS03515"
                     /old_locus_tag="pgaptmp_000703"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(17842..17844),aa:Gly,seq:gcc)
     gene            complement(18088..18633)
                     /gene="orn"
                     /locus_tag="ACHOSC_RS03520"
                     /old_locus_tag="pgaptmp_000704"
     CDS             complement(18088..18633)
                     /gene="orn"
                     /locus_tag="ACHOSC_RS03520"
                     /old_locus_tag="pgaptmp_000704"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020882950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000175 - 3'-5'-RNA exonuclease activity
                     [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligoribonuclease"
                     /protein_id="HGU4546697.1"
                     /translation="MSANENNLIWIDLEMTGLDPERDRIIEIATLVTDANLNILAEGP
                     TIAVHQSDEQLALMDDWNVRTHTASGLVERVKASTMGDREAELATLEFLKQWVPAGKS
                     PICGNSIGQDRRFLFKYMPELEAYFHYRYLDVSTLKELARRWKPEILDGFTKQGTHQA
                     MDDIRESVAELAYYREHFIKL"
     gene            18728..19780
                     /gene="rsgA"
                     /locus_tag="ACHOSC_RS03525"
                     /old_locus_tag="pgaptmp_000705"
     CDS             18728..19780
                     /gene="rsgA"
                     /locus_tag="ACHOSC_RS03525"
                     /old_locus_tag="pgaptmp_000705"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313167.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="small ribosomal subunit biogenesis GTPase RsgA"
                     /protein_id="HGU4546698.1"
                     /translation="MSKNKLSKGQQRRVNANHQRRLKTSKEKPDYDDNLFGEPDEGIV
                     ISRFGMHADVESADGDVHRCNIRRTIRSLVTGDRVVWRPGKPAAEGVNVKGIVEAVHE
                     RTSVLTRPDFYDGVKPIAANIDQIVIVSAILPELSLNIIDRYLVACETLQIEPIIVLN
                     KIDLLDDEGMAFVNEQMDIYRNIGYRVLMVSSHTQDGLKPLEEALTGRISIFAGQSGV
                     GKSSLLNALLGLQKEILTNDVSDNSGLGQHTTTAARLYHFPHGGDVIDSPGVREFGLW
                     HLEPEQITQGFVEFHDYLGLCKYRDCKHDTDPGCAIREAVEEGKIAETRFENYHRILE
                     SMAQVKTRKNFSDTDD"
     gene            19877..20845
                     /gene="asd"
                     /locus_tag="ACHOSC_RS03530"
                     /old_locus_tag="pgaptmp_000706"
     CDS             19877..20845
                     /gene="asd"
                     /locus_tag="ACHOSC_RS03530"
                     /old_locus_tag="pgaptmp_000706"
                     /EC_number="4.1.1.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313166.1"
                     /note="Phosphatidylserine decarboxylase is synthesized as
                     a single chain precursor. Generation of the pyruvoyl
                     active site from a Ser is coupled to cleavage of a Gly-Ser
                     bond between the larger (beta) and smaller (alpha chains).
                     It is an integral membrane protein; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0004609 - phosphatidylserine decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="archaetidylserine decarboxylase"
                     /protein_id="HGU4546699.1"
                     /translation="MLNSFKLSLQYILPKLWLTRLAGWGASKRAGWLTKLVIDLFVKY
                     YKVDMKEAQKPDTASYRTFNEFFVRPLRDEVRPIDTDPNVLVMPADGVISQLGKIEED
                     KILQAKGHNYSLEALLAGNYLMADLFRNGTFVTTYLSPRDYHRVHMPCNGILREMIYV
                     PGDLFSVNHLTAQNVPNLFARNERVICLFDTEFGPMAQILVGATIVGSIETVWAGTIT
                     PPREGIIKRWTWPAGENDGSVALLKGQEMGRFKLGSTVINLFAPGKVNLVEQLESLSV
                     TKIGQPLAVSTETFVTPDAEPAPLPAEEIEAEHDASPLVDDKKDQV"
     gene            20867..24190
                     /gene="mscM"
                     /locus_tag="ACHOSC_RS03535"
                     /old_locus_tag="pgaptmp_000707"
     CDS             20867..24190
                     /gene="mscM"
                     /locus_tag="ACHOSC_RS03535"
                     /old_locus_tag="pgaptmp_000707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="miniconductance mechanosensitive channel MscM"
                     /protein_id="HGU4546700.1"
                     /translation="MRLIITFLMAWCLSWGAYAATAPDSKQITQELEQAKAAKPAQPE
                     VVEALQSALNALEERKGSLERIKQYQQVIDNYPKLSATLRAQLNNMRDEPRSVSPGMS
                     TDALNQEILQVSSQLLDKSRQAQQEQERAREIADSLNQLPQQQTDARRQLNEIERRLG
                     TLTGNTPLNQAQNFALQSDSARLKALVDELELAQLSANNRQELARLRSELAEKESQQL
                     DAYLQALRNQLNSQRQLEAERALESTELLAENSADLPKDIVAQFKINRELSAALNQQA
                     QRMDLVASQQRQATSQTLQVRQALNTLREQSQWLGSSNLLGEALRAQVARLPEMPKPQ
                     QLDTEMAQLRVQRLRYEDLLNKQPLLRQIHQADGQPLTAEQNRILEAQLRTQRELLNS
                     LLQGGDTLLLELTKLKVSNGQLEDALKEVNEATHRYLFWTSDVRPMTIAWPLEIAQDL
                     RRLISLDTFSQLGKASVMMLTSKETILPLFGALILVGCSIYSRRYFTRFLERSAAKVG
                     KVTQDHFWLTLRTLFWSILVASPLPVLWMTLGYGLREAWPYPLAVAIGDGVTATVPLL
                     WVVMICATFARPNGLFIAHFGWPRERVSRGMRYYLMSIGLIVPLIMALMMFDNLDDRE
                     FSGSLGRLCFILICGALAVVTLSLKKAGIPLYLNKEGSGDNITNHMLWNMMIGAPLVA
                     ILASAVGYLATAQALLARLETSVAIWFLLLVVYHVIRRWMLIQRRRLAFDRAKHRRAE
                     MLAQRARGEEEAHHHSSPEGAIEVDESEVDLDAISAQSLRLVRSILMLIALLSVIVLW
                     SEIHSAFGFLENISLWDVTSTVQGVESLEPITLGAVLIAILVFIITTQLVRNLPALLE
                     LAILQHLDLTPGTGYAITTITKYLLMLIGGLVGFSMIGIEWSKLQWLVAALGVGLGFG
                     LQEIFANFISGLIILFEKPIRIGDTVTIRDLTGSVTKINTRATTISDWDRKEIIVPNK
                     AFITEQFINWSLSDSVTRVVLTIPAPADANSEEVTEILLTAARRCSLVIDNPAPEVFL
                     VDLQQGIQIFELRIYAAEMGHRMPLRHEIHQLILAGFHAHGIDMPFPPFQMRLESLNG
                     KQTGRTLTSAGKGRQAGSL"
     gene            complement(24219..24533)
                     /gene="yjeO"
                     /locus_tag="ACHOSC_RS03540"
                     /old_locus_tag="pgaptmp_000708"
     CDS             complement(24219..24533)
                     /gene="yjeO"
                     /locus_tag="ACHOSC_RS03540"
                     /old_locus_tag="pgaptmp_000708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjeO family protein"
                     /protein_id="HGU4546701.1"
                     /translation="MSARMFVLCCIWFIVAFLWITITSALDKEWMIDGRGINNVCDVL
                     MYLEEDDTRDVGVIMTLPLFFPFLWFALWRKKRGWFMYATALAIFGYWLWQFFLRYQF
                     CL"
     gene            complement(24530..24844)
                     /gene="yjeN"
                     /locus_tag="ACHOSC_RS03545"
                     /old_locus_tag="pgaptmp_000709"
     CDS             complement(24530..24844)
                     /gene="yjeN"
                     /locus_tag="ACHOSC_RS03545"
                     /old_locus_tag="pgaptmp_000709"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YjeN"
                     /protein_id="HGU4546702.1"
                     /translation="MDDTSRDPAITEDEIRALQFSAGDVAEIEQTILSFVDACHTRKV
                     AMVVGSTINTLKDRDGKRWGNLPDIYCAYLIRCLVFRGELVGYGDLFRMRYSEIKRPV
                     TL"
     gene            complement(24896..26398)
                     /gene="yjeM"
                     /locus_tag="ACHOSC_RS03550"
                     /old_locus_tag="pgaptmp_000710"
     CDS             complement(24896..26398)
                     /gene="yjeM"
                     /locus_tag="ACHOSC_RS03550"
                     /old_locus_tag="pgaptmp_000710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418580.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate/gamma-aminobutyrate family transporter
                     YjeM"
                     /protein_id="HGU4546703.1"
                     /translation="MPHTIKKMSLIGLILMIFTSVFGFANSPSAYYLMGYSAIPFYIF
                     SALLFFIPFALMMAEMGAAYRKEEGGIYSWMNNSVGPRFAFIGTFMWFSSYIIWMVST
                     SAKVWVPFSTFLYGSDMTQHWRIAGLEPTQVVGLLAVAWMILVTVVASKGINKIARIT
                     AVGGIAVMCLNLVLLLVSITILLLNGGHFAQDINFLASPNPGYQSGLAMLSFVVFAIF
                     AYGGIEAVGGLVDKTENPEKNFAKGIVFAAIVISIGYSLAIFLWGLSTNWQQVLSNGS
                     VNLGNITYVLMKSLGVTLGNALHLSPEASLSLGVWFARITGLSMFLAYTGAFFTLCYS
                     PLKAIIQGTPKALWPEPMTRLNAMGMPSIAMWMQCGLVTVFILLVSFGGGTASAFFNK
                     LTLMANVSMTLPYLFLALAFPFFKARQDLDRPFVIFKTHMSAMIATVVVVLVVTFANV
                     FTIIQPVVEAGDWDSTLWMIGGPVFFSLLAMAIYQNYCSRVAKNPQWAVE"
     gene            complement(26617..27594)
                     /gene="epmA"
                     /locus_tag="ACHOSC_RS03555"
                     /old_locus_tag="pgaptmp_000711"
     CDS             complement(26617..27594)
                     /gene="epmA"
                     /locus_tag="ACHOSC_RS03555"
                     /old_locus_tag="pgaptmp_000711"
                     /EC_number="6.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000004771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P--(R)-beta-lysine ligase"
                     /protein_id="HGU4546704.1"
                     /translation="MSETASWQPSASIPNLLKRAAIMAEIRRFFADRGVLEVETPCMS
                     QATVTDIHLVPFETRFVGPGHSQGMNLWLMTSPEYHMKRLLVAGCGPVFQLCRSFRNE
                     EMGRYHNPEFTMLEWYRPHYDMYRLMNEVDDLLQQVLDCPAAESLSYQQAFLRYLEID
                     PLSADKTQLREVAAKLDLSNVADTEEDRDTLLQLLFTFGVEPNIGKEKPTFVYHFPAS
                     QASLAQISTEDHRVAERFEVYYKGIELANGFHELTDAREQQQRFEQDNRKRAARGLPQ
                     HPIDQNLIEALKVGMPDCSGVALGVDRLVMLALGAETLAEVIAFSVDRA"
     gene            27919..29727
                     /gene="frdA"
                     /locus_tag="ACHOSC_RS03560"
                     /old_locus_tag="pgaptmp_000712"
     CDS             27919..29727
                     /gene="frdA"
                     /locus_tag="ACHOSC_RS03560"
                     /old_locus_tag="pgaptmp_000712"
                     /EC_number="1.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0009061 - anaerobic respiration [Evidence
                     IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate reductase (quinol) flavoprotein
                     subunit"
                     /protein_id="HGU4546705.1"
                     /translation="MQTFQADLAIVGAGGAGLRAAIAAAQANPNAKIALISKVYPMRS
                     HTVAAEGGSAAVAQDHDSFEYHFHDTVAGGDWLCEQDVVDYFVHHCPTEMTQLELWGC
                     PWSRRPDGSVNVRRFGGMKIERTWFAADKTGFHMLHTLFQTSLQFPQIQRFDEHFVLD
                     ILVDDGHVRGVVAMNMMEGTLVQIRANAVVMATGGAGRVYRYNTNGGIVTGDGMGMAL
                     SHGVPLRDMEFVQYHPTGLPGSGILMTEGCRGEGGILVNKNGYRYLQDYGMGPETPLG
                     EPKNKYMELGPRDKVSQAFWHEWRKGNTISTPRGDVVYLDLRHLGEKKLHERLPFICE
                     LAKAYVGVDPVKEPIPVRPTAHYTMGGIETDQNCETRIKGLFAVGECSSVGLHGANRL
                     GSNSLAELVVFGRLAGEQATERAATAGNGNEAAIEAQAAGVEQRLKDLVNQDGGENWA
                     KIRDEMGLAMEEGCGIYRTPELMQKTIDKLAELQERFKRVRITDTSSVFNTDLLYTIE
                     LGHGLNVAECMAHSAMARKESRGAHQRLDEGCTERDDVNFLKHTLAFRDADGTTRLEY
                     SDVKITTLPPAKRVYGGEADAADKAEAANKKEKANG"
     gene            29720..30454
                     /gene="frdB"
                     /locus_tag="ACHOSC_RS03565"
                     /old_locus_tag="pgaptmp_000713"
     CDS             29720..30454
                     /gene="frdB"
                     /locus_tag="ACHOSC_RS03565"
                     /old_locus_tag="pgaptmp_000713"
                     /EC_number="1.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020882957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate reductase iron-sulfur protein"
                     /protein_id="HGU4546706.1"
                     /translation="MAEMKNLKIEVVRYNPEVDTAPHSAFYEVPYDATTSLLDALGYI
                     KDNLAPDLSYRWSCRMAICGSCGMMVNNVPKLACKTFLRDYTDGMKVEALANFPIERD
                     LVVDMTHFIESLEAIKPYIIGNSRTADQGTNIQTPAQMAKYHQFSGCINCGLCYAACP
                     QFGLNPEFIGPAAITLAHRYNEDSRDHGKKERMAQLNSQNGVWSCTFVGYCSEVCPKH
                     VDPAAAIQQGKVESSKDFLIATLKPR"
     gene            30465..30860
                     /gene="frdC"
                     /locus_tag="ACHOSC_RS03570"
                     /old_locus_tag="pgaptmp_000714"
     CDS             30465..30860
                     /gene="frdC"
                     /locus_tag="ACHOSC_RS03570"
                     /old_locus_tag="pgaptmp_000714"
                     /EC_number="1.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate reductase subunit FrdC"
                     /protein_id="HGU4546707.1"
                     /translation="MTTKRKPYVRPMTSTWWKKLPFYRFYMLREGTAVPAVWFSIELI
                     FGLFALKNGPEAWAGFVDFLQNPVIVIINLITLAAALLHTKTWFELAPKAANIIVKDE
                     KMGPEPIIKSLWAVTVVATIVILFVALYW"
     gene            30871..31230
                     /gene="frdD"
                     /locus_tag="ACHOSC_RS03575"
                     /old_locus_tag="pgaptmp_000715"
     CDS             30871..31230
                     /gene="frdD"
                     /locus_tag="ACHOSC_RS03575"
                     /old_locus_tag="pgaptmp_000715"
                     /EC_number="1.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate reductase subunit FrdD"
                     /protein_id="HGU4546708.1"
                     /translation="MINPNPKRSDEPVFWGLFGAGGMWSAIIAPVMILLVGILLPLGL
                     FPGDALSYERVLAFAQSFIGRVFLFLMIVLPLWCGLHRMHHAMHDLKIHVPAGKWVFY
                     GLAAILTVVTLIGIVTI"
     gene            31293..32426
                     /gene="ampC"
                     /locus_tag="ACHOSC_RS03580"
                     /old_locus_tag="pgaptmp_000716"
     CDS             31293..32426
                     /gene="ampC"
                     /locus_tag="ACHOSC_RS03580"
                     /old_locus_tag="pgaptmp_000716"
                     /EC_number="3.5.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001336292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008800 - beta-lactamase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BlaEC family class C beta-lactamase"
                     /protein_id="HGU4546709.1"
                     /translation="MFKTTLCALLITASCSTFAAPQQINDIVHRTITPLIEQQKIPGM
                     AVAVIYQGKPYYFTWGYADIAKKQPVTQQTLFELGSVSKTFTGVLGGDAIARGEIKLS
                     DPTTKYWPELTAKQWNGITLLHLATYTAGGLPLQVPDEVKSSSDLLRFYQNWQPAWAP
                     GTQRLYANSSIGLFGALAVKPSGLSFEQAMQTRVFQPLKLNHTWINVPPAEEKNYAWG
                     YREGKAVHVSPGALDAETYGVKSTIEDMACWVRSNMNPRDINDKTLQQGIQLAQSRYW
                     QTGDMYQGLGWEMLDWPVNPDSIINGSGNKIALAAHPVKAITPPTPAVRASWVHKTGA
                     TGGFGSYVAFIPEKELGIVMLANKNYPNPARVAAAWQILNALQ"
     gene            32515..33048
                     /gene="blc"
                     /locus_tag="ACHOSC_RS03585"
                     /old_locus_tag="pgaptmp_000717"
     CDS             32515..33048
                     /gene="blc"
                     /locus_tag="ACHOSC_RS03585"
                     /old_locus_tag="pgaptmp_000717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipocalin Blc"
                     /protein_id="HGU4546710.1"
                     /translation="MRLLPLVAAATAAFLVVACSSPTPPRGVTVVNNFDAKRYLGTWY
                     EIARFDHRFERGLEKVTATYSLRDDGGLNVINKGYNPDRGMWQQSEGKAYFTGAPTRA
                     ALKVSFFGPFYGGYNVIALDREYRHALVCGPDRDYLWILSRTPTISDEVKQEMLAVAT
                     REGFDVSKFIWVQQPGS"
     gene            complement(33045..33362)
                     /gene="sugE"
                     /locus_tag="ACHOSC_RS03590"
                     /old_locus_tag="pgaptmp_000718"
     CDS             complement(33045..33362)
                     /gene="sugE"
                     /locus_tag="ACHOSC_RS03590"
                     /old_locus_tag="pgaptmp_000718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418572.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="quaternary ammonium compound efflux SMR
                     transporter SugE"
                     /protein_id="HGU4546711.1"
                     /translation="MSWIILVIAGLLEVVWAVGLKYTHGFSRLTPSVITVTAMIVSMA
                     LLAWAMKSLPVGTAYAVWTGIGAVGAAITGIVLLGESANPMRLASLALIVLGIIGLKL
                     STH"
     gene            complement(33544..33690)
                     /gene="ecnB"
                     /locus_tag="ACHOSC_RS03595"
                     /old_locus_tag="pgaptmp_000719"
     CDS             complement(33544..33690)
                     /gene="ecnB"
                     /locus_tag="ACHOSC_RS03595"
                     /old_locus_tag="pgaptmp_000719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein toxin entericidin B"
                     /protein_id="HGU4546712.1"
                     /translation="MVKKTIAAIFSVLVLSTVLTACNTTRGVGEDISDGGNAISGAAT
                     KAQQ"
     gene            complement(33801..33926)
                     /gene="ecnA"
                     /locus_tag="ACHOSC_RS03600"
                     /old_locus_tag="pgaptmp_000720"
     CDS             complement(33801..33926)
                     /gene="ecnA"
                     /locus_tag="ACHOSC_RS03600"
                     /old_locus_tag="pgaptmp_000720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein antitoxin entericidin A"
                     /protein_id="HGU4546713.1"
                     /translation="MMKRLIVLVLLASTLLTGCNTARGFGEDIKHLGNSISRAAS"
     gene            complement(33978..34544)
                     /gene="efp"
                     /locus_tag="ACHOSC_RS03605"
                     /old_locus_tag="pgaptmp_000721"
     CDS             complement(33978..34544)
                     /gene="efp"
                     /locus_tag="ACHOSC_RS03605"
                     /old_locus_tag="pgaptmp_000721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004871142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P"
                     /protein_id="HGU4546714.1"
                     /translation="MATYYSNDFRAGLKIMLDGEPYAVEASEFVKPGKGQAFARVKLR
                     RLLTGTRVEKTFKSTDSAEGADVVDMNLTYLYNDGEFWHFMNNETFEQLSADAKAIGD
                     NAKWLLDQAECIVTLWNGQPISVTPPNFVELEIVDTDPGLKGDTAGTGGKPATLSTGA
                     VVKVPLFVQIGEVIKVDTRSGEYVSRVK"
     gene            34586..35614
                     /gene="epmB"
                     /locus_tag="ACHOSC_RS03610"
                     /old_locus_tag="pgaptmp_000722"
     CDS             34586..35614
                     /gene="epmB"
                     /locus_tag="ACHOSC_RS03610"
                     /old_locus_tag="pgaptmp_000722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0072581 - protein-N6-(L-lysyl)-L-lysine
                     modification to protein-N6-(beta-lysyl)-L-lysine [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EF-P beta-lysylation protein EpmB"
                     /protein_id="HGU4546715.1"
                     /translation="MAHIVTLNTPSREDWLTQLADVVTDPDELLRLLNIDADEKLLAG
                     RSAKKLFALRVPRSFIDRMEKGNPDDPLLRQVLTSQDEFVVASGFSTDPLEEQHSVVP
                     GLLHKYHNRALLLVKGGCAVNCRYCFRRHFPYAENQGNKRNWQTALEYVAAHPELDEM
                     IFSGGDPLMAKDHELDWLLTQLEAIPHIKRLRIHSRLPIVIPARITEALVERFARSTL
                     QILLVNHINHANEVDETFRQAMAKLRRVGVTLLNQSVLLRGVNDNAQTLANLSNALFD
                     AGVMPYYLHVLDKVQGAAHFMVSDDEARQIMRELLTLVSGYLVPKLAREIGGEPSKTP
                     LDLQLRQQ"
BASE COUNT         8535 a         9858 c         9109 g         8367 t
ORIGIN      
        1 gtttaaatat taataaaatg aatatttgca gtacgtaatt atcttaccag ctatagacaa
       61 aaaaaaacca tccaaatctg gatggctttt cataattcag acgaactagc tgcgctgacg
      121 aaccgcttca aataagcaaa ttccggttgc aaccgaaacg ttcagggaag aaacacttcc
      181 tgccattggg atgctgatca actcatcgca atgttcacgg gtcaggcgac gcataccttc
      241 accttccgcg cccatcacca gcgccaggcg tccggtcatt ttgctctgat agagtgtatg
      301 atccgcctcg cctgccgtac cgacgatcca gatattctct tcctgcaaca tacgcatggt
      361 gcgcgcaagg ttagtcaccc gaatcagtgg aacgctttct gccgcgccgc aggctacttt
      421 tttcgccgta gcgttgagct gtgcggagcg atctttcggc acaatcaccg cgtgaacgcc
      481 agcagcgtcc gcgctacgca ggcacgcgcc gaggttgtgc ggatcggtta caccgtcgag
      541 gatcagcagg aacggttgat cgagcgaagc gatcagatcc ggcagatcgt tttcctgata
      601 ctgacgtcct ggcttcacgc gggcgataat gccctgatgc acggcaccgt cgcttttctc
      661 gtcgagatat tggcggtttg ccaactggat aaccacgccc tgagactcaa gggcgtgaat
      721 cagcggtaac agacgtttat cttcacggcc ttttaaaata aagacttcct gaaaacgttc
      781 aggggcgcgc tccagcaggg cctgcactgc gtggatgccg taaatcattt cgctcattaa
      841 tgtactcgtt gtttacgttt tgcctgatgc actacgttta tccggcctac atgatctctg
      901 caatatattg aatttgcgtg attatgtagg ccggataagg cgttcacgcc gcatccggca
      961 tgaacaaagc gcattttgtc agcaatctaa ccctcttctt ttaaagaggg tattgatcac
     1021 tctgccactt ttttcttcgc cgcacgcttc gctttggtcg ctgcggctat tttctgcgtt
     1081 ttcgccgatg gctttttcgc ttttctcgcg tctttcttcg ccgctttcgg cttcgttttt
     1141 ttctcaccgc ggaaggcgct gtctggctca aagtttacct ttttaccgac ctgacgacgc
     1201 ttgccgcctt ttttgcctgc atcgcctttt ttcgctttct cgcgcgccgt tttaccgacg
     1261 ttgcgcggtg cgcgttcact ggagatcaga ctaaagtcga ttttgcgctc gtccatatta
     1321 accgcttcga ctcgaacttc cacgcgatcg cccaggcgat aagtctggcc gctggattcc
     1381 cccatcaggc gttgccctac ctggtcaaag cgatagtagt cattgtccag cgaagagaca
     1441 tggaccagac catcaatgaa caagtcgtcc agacggacga agaagccaaa gccagtgacg
     1501 ctggaaatta cgcctttaaa gacgttacct acctggtcga gcatgaagtc acacttcagc
     1561 cagtcagcga catcgcgcgt tgcttcgtcg gcacgacgtt ccgccatcga acagtgctga
     1621 cccagttgca acatctcttc catcgaataa tggtagccgc cggtttcagt ggtgttgccc
     1681 tgatgcccct gctctttcgc cagcagatat ttaatggcgc ggtgcagcgt caggtcagga
     1741 taacgacgaa tcggcgaagt aaagtgcgca taggactgca atgccagacc gaagtgacca
     1801 cggttttctg gatcgtaaat cgcctgtttc atcgagcgta gcagcatggt ttgcagcatt
     1861 tctgcatcag gacggtcggc aaccgactcc agcagttccg cgtagtcacg cggttccggc
     1921 ttgttaccac ccggcagctc cagccccagc tccgccagca ctgaacggaa agaggtaatc
     1981 gcttcggtgc tcggcttgtc gtgaatacgg aacagtgccg gttctttcgc tttctcaacg
     2041 aaacgcgccg ccgagatatt cgccagaatc atgcactctt caattaattt gtgcgcgtcg
     2101 ttacgctggg tctgttcgat acgttcaata cggcgttcag cgttgaaaat gaacttcgct
     2161 tcttcgctct caaatgagat cccaccgcgt tcttcacggg ctttatccag cactttatag
     2221 aggttatgca actcttcgag atgcttaacc agcggggcgt actgctcgcg cagatcctga
     2281 tcgccctgca gaatatgcca gactttggtg taggtcagac gcgcgtgaga gctcatcacc
     2341 gcttcgtaga atttgtagcc cgtcaggcgg cctttcgacg aaaccgtcat ctcgcacacc
     2401 atacacaggc ggtctacctg cgggttgagc gaacacaggc cgttagagag cacttccggc
     2461 agcatcggga taacctgcga agggaagtac accgacgtgc cacggttacg cgcttctctg
     2521 tccagcggcg ttggcggacg cacatagtag ctgacgtcgg caatcgcgac ccataaacgc
     2581 cagccgccgc cgcgtttttt ctcgcagtaa actgcatcgt caaagtcacg ggcgtcttcg
     2641 ccatcaatgg tgaccagcgg taaatcgcgc agatcaacac ggcccgcttt tgcttcttcc
     2701 ggcacttctt ctttcagccc ggcaacctgt tgctcaacag cctgcggcca gatgtacgga
     2761 atttcatggg tacgcagagc gatatcaacc gccatgccgg tgcccatatt gtcgcccagc
     2821 acttcgacga ttttacccac cgctttggtg cggcgagtcg gacgctgagt cagttcgact
     2881 acgaccacaa agcccatccg cgcgcccatg atctgatcgg gcgggattaa gatatcgaag
     2941 ctcagacggc tatcgtcagg aaccacaaag ccgacgcccg cttcagtaaa gtagcgacca
     3001 acaatctggc tggtttttgg caccagtacg cggacaatac gcgcttcacg acgaccttta
     3061 cggtcagcgc ccagcggctg cgccagcacc tgatcgccat gaatgcaggt tttcatctgc
     3121 tcgctggaga gatacaaatc atctttacgc ccttcaaccc gcagaaagcc gtagccatca
     3181 cggtggccaa taacggtacc tttcaccagg tcgaggcgtt ccggcagcgc atagcactga
     3241 cgacgagtga agaccagttg accatcgcgc tccatcgcgc gcaggcggcg acgcaggcct
     3301 tcaagctgct cttcgccttc aatgtgcagt tctaccgcca gctcatcacg gctggccggt
     3361 ttttcacgtt tggttaaatg ttcgaggata aattcccgac tagggatggg attcgcgtat
     3421 ttttcagctt cgcgttcctg gaaaggatct tgtgacatct cggttcctcc gttgtcatct
     3481 ctgatgaaga ttttcgtcac tccaccagca ataatttata aagcggttga ttctcttcaa
     3541 ccaaatcggc aagcgtgtag ttatccagtt ccgtaagaaa actttgcacg gccttagaaa
     3601 gcgcctgttt caaccgacag gcaggtgtaa tgtggcaaaa ctcactgctg caattcacca
     3661 gcgataaggg ctccagctcg cgcaccacat caccaatacg tatcgcactc gccggtttac
     3721 ccaggcgaat gccgccattt tttccacgaa cagcagtcac gtagccggca cgactaagtt
     3781 gattgattat tttgaccata tgattacggg agacgccgta gacgtcagtc acttcagaaa
     3841 tactggtcat ccgcccttct ggcaatgacg ccatgtagat cagcgcacgt aatccgtaat
     3901 cagtgaaact cgttaactgc acatcaacct caaaagggaa atcgggaaaa aatacattta
     3961 tattgatgat aaaccagcca caagctgtgt cgctaattta tttcagttcg gggaaggaaa
     4021 aaagcgagga tttaacactg tgtcggatag cgtaatggct taaccgacac agcaatgtat
     4081 cacactgtta gcccggcagg caaaatatct gccaggcgta ccagaattac gcgtcgaacg
     4141 gatcgcgcag aatcatggtt tcagtacgat ccggaccggt agagatgata tcgatcggca
     4201 caccagtcag ctcttcaata cgcttgatgt agttcagcgc cgcctgcggc agaccgctac
     4261 gatctttcac gccgaaggtg gattcagacc agcccggcat ggtttcgtaa atcggctcta
     4321 cacctttcca gtcgtcagct gccagcggag tggtcgtcac ttcacgacca tccggcatac
     4381 ggtaagccac acagagtttc acctctttca ggccatccag aacgtccagt ttagtcaggc
     4441 agaagccaga cagggagttc agctgtaccg cacgacgaac ggcaacggtg tccagccagc
     4501 cggtacgacg acgacgcccc gtagttgcgc cgaattcgtt accctgcttg cagaggaact
     4561 cgccagtttc atcaaacagt tcggtcggga acggacctgc acctacacga gttgagtaag
     4621 ctttgaggat acccagaaca taatcaacat aacgcgggcc caggccggaa ccggtcgcca
     4681 cgccaccagc agtggtgttg gaagaagtta cgtacggata agtaccgtgg tcgatatcca
     4741 gcagcgtacc ctgcgcacct tcaaacatga cgaaatcgcc acgctgacgc gcctggtcga
     4801 gcaggtcaga aacgtcaacc accatagaag tcaggatgtc ggcaacagcc atcgtatcat
     4861 ccagaacttt ctggtaatca accgcttcag ctttgtagta gttaaccaac tggaagttgt
     4921 gatattccat cacttctttc agtttttcag cgaaggtttc tttgtcgaaa aggtcgccaa
     4981 cacgcagacc gcgacgtgct actttatctt cataagcagg cccgatacca cgaccggtgg
     5041 tgccgatcgc tttcgcgcca cgcgctttct cacgcgcgtt atccagcgca acgtgataat
     5101 caaggatcag cggacatgct tcagacagca gcagacgctc acgaacgggg atgccacggt
     5161 cttccagttc tttcatctct ttcatcagcg cggccggaga cagcacaaca ccgttaccga
     5221 tgatgctggt tacattctcg cggagaatac ctgatggaat aagatggaga acggtttttt
     5281 caccgttgat tacgagagta tggcctgcgt tgtgaccgcc ctggtagcgt acaacatatt
     5341 tagcccgttc agtcagaaga tcgacgatct tacctttacc ttcgtcaccc cattgggtgc
     5401 ccagtacgac gacgttgtta cccatttttc aaaatcaccg tttgcttaaa aatggattct
     5461 accatcgctt tttcagagtt acagcacttt ttgcactcaa aaatgacctc gatcagtctg
     5521 ttttttgttc agccaatcgt tttcctcaac atgtagtaga ccacaacgcc cgcaaccaca
     5581 agtccaccgc caaaacgacg taaaatatta tcgggcaaat tggtcatcgc agagatcatc
     5641 ttcttccatg ccttcgggta aagcatcggc cctaaacctt ccagtaccaa aaccagggca
     5701 agcgccagcc agattgtcga attcattcat tatccttata gaaaaagaaa accaccgaca
     5761 tccctgagga tgcggtggct ttattgacct gtaccgcagt cgttatatta acgcgttgcg
     5821 gaagtcggcg tcttcatgta gcggaagaaa tcgctatccg ggctcatgac catcacgtcc
     5881 tgattgccag agaagctgtt ctcataagca cgcaggctac ggatgaatgc gtagaagtcc
     5941 ggatctttac tgaatgcatc agcaaacagt ttggctgctt cggcatcgcc ttcaccacgc
     6001 atgatgcggc cctgacgctc agcttctgcc agcgttctgg tcacttcata gtcggcagtc
     6061 gcgcgcagtt tttccgcttc ttcctgacct tgtgaacggt gacgacgcgc taccgcttca
     6121 cgctcggcgc gcatacggtt gtagatcgct tcagacactt cggtcggcag gttgatctgc
     6181 ttgatacgca catcgacaac ttcaataccc agcgccgcca tactgttcgg gttgatgacc
     6241 ggaactttgc ccttcgtctc agccgttacg cgctctgccg cttcggcaat ggcgttatct
     6301 gctgccgggg tagtaacttc atcttctgta cccgcagaac cggagttcag cgcgtcacgt
     6361 acttcgaggg tcagacgacc acgggaatca gtgacgatat ctttcacgtc caggcgacca
     6421 atttcagaac gcagacggtc agagaactta cgtttcaaca gcacttccgc ttgcgaaatg
     6481 tcgccaccac ccgttgccag gtagtaacgg ctgaaatcgc tgatgcgcca tttgatgtaa
     6541 gaatcgacga tcaggtcttt cttctctttg gtcacaaagc ggtcggcctg gttgtccatg
     6601 gtctgaatac gtgcgtcgag cattttcacc gtttcaatga acggtatctt gaaatgcaga
     6661 cccggctcat aaaccagagg tttgttgtca tcgtcacgca gtaccttacc aaaacgcagc
     6721 gtaataccgc gctcaccttc tttgacgaca aagacagaca tgtaaagcac taccagcacg
     6781 atgatgataa tcgcgataac tgacttacgc atcgttattc cccctgacgc tggtagtcgt
     6841 tacgctgcgc gttggcgcgg cgttggtcca taatatcgcc ctgactggtg gacgacgtgt
     6901 tgcttgctcc actggttgtg gaggaagagg ctggcggcag acgcagcaga ttgctggcac
     6961 cgttatcgct cttcgccgca ggggcgttac cacctttcag catctggtct aacggcagaa
     7021 ccatcaggtt tccacctttg tcgttaacca gcactttgcg ggtgttgccc aacacttttt
     7081 ccatcgtctc gatatacaga cgctcgcgag taatttccgg cgcggcttta tattccggca
     7141 gaagtttagc aaagcgcgcc acttcaccct gagcttccag gatggtctgg gccttgtacg
     7201 cacgcgcctc ttcgaggata cgttgcgcct gaccgttcgc acgcggctga acttcgttgg
     7261 tatacgcttc tgcttcacga atgtattgct gttcgttttc acgcgcggca atcgcatcgt
     7321 caaacgccgc ttttacttct tccggcggac gagcagcctg gaagttgacg tccagcagcg
     7381 tgatacccat gtcatacgga cgaatcgtct cttccagttc gcgctgagta tcgctacgaa
     7441 tcacggtacg accttccgtc agaatgcggt ccatggtgta tttaccgata actccacgca
     7501 gggcgctgtc ggtagcctga cgcaggctgt catccgggct ggtcacgcta tacagatatt
     7561 tttccggatt ggtgacgcgg tactgcacgt tcatctcaac gcgcactacg ttctcgtccg
     7621 acgtcagcat cacaccagaa gcggccagtt cacgcacggc ttccacgttc accggtttga
     7681 cttcgtcgat aaacgtcggt ttccagttca gacccggctc aaccagatgg ctgaatttac
     7741 caaagcgtgt taccacgccg cgttcggctt ctttaatggt atagaaacca ctggccgccc
     7801 agataatgac aatcgctgcc gctgcgatgg taacgacacg accgccaagc tgcgggcgcg
     7861 ggccttgcga tgaactgcca ccgccagatc cggtgccttt accgccgccc agaccaccga
     7921 gctttttgct cagtttgcgg aagatatcat ctaaatcagg tggcccttga tcgcgaccgc
     7981 ctttgtttcc atttccctca gagttgccgc caggtttgct gcttccccac gggtcgcggt
     8041 cttgtccgtt attaccgggc tgattccacg ccatgtttgt gctccatatt tgttatgcgg
     8101 tgatccctgt tgtctttcga cgccagagga tatgactcca cgcttcagac gctacgccgt
     8161 tagatcaggt aatcgatcaa cgccggttct tgtttacaga ggcgacgcca gtcaacgatc
     8221 ggcatacgaa cttgcagact tacgctgccg tcctcctcca tccactcttt ttctattgcc
     8281 tgaagctgat aaaaacgact tctcagacgc ccttcctgcg gtggcagacg caatgtatgc
     8341 tgcgccacct cgccggaaag ccgccccgtc aaagcctgaa aaagctgtgg tatccccgct
     8401 ccggtctgtg cggaaagcca gacacggatc ggtttgttct cttcgtcccg atcaatacgc
     8461 ggttcgaaat cttccagcat atcgatcttg ttcatcacca gcagggttgg gatctcgtga
     8521 gcgtcgatct cttcaagaac cgtattcacc gcttcgatgt tttcttgtac acgcacatcc
     8581 gccgcatcaa tgacgtgcag cagtaatgtg gcttgccgcg tctcttgtaa cgtggcttta
     8641 aatgccgcca ccagatcgtg cggcaggtgg cgaataaacc ctacggtatc tgcaagtacg
     8701 gtttcaccga catctgcaac gtcaatacgc cgcaacgtcg ggtcgagggt ggcaaacaac
     8761 tggtctgccg cgtagacccg cgcttcggtg atgcgattga aaagggtaga tttaccggcg
     8821 ttggtatatc ccaccagcga aacagtagga acgtcggctt tgatacgcga ttgccgcccc
     8881 tgctcacgct gcttttcaac tctttccagg cgtgactgta tctgcacgat gcgattacgc
     8941 aacaaacgac ggtcggtttc gagctgggtt tcacccggac cacgcaaacc tatcccgcct
     9001 ttctgtcttt caaggtgggt ccagccacgc accaggcgcg tagccagatg gcgcagctgc
     9061 gccagctcaa cctgcaactt accctcatgg gtacgcgcac gttgggcgaa aatatctaaa
     9121 ataaggccgg tgcggtcgat aacacgacac tcgcacaaac gctccaggtt acgctcttgc
     9181 gccgggctca gggcatggtc aaaaagaacg accgaagcac ccgtcgcttt gacagcttcc
     9241 gcaatttcaa ctgctttacc ttcacctaca aaatactttg ggtgcggcgc tttacggcta
     9301 ccggtaatca cctgcaatgc ttcgacaccg gcggaagaga ccagagattc aaactcctgg
     9361 aggtcttcca tatctttgtc ttgcgtaaaa tagatgtgta ccagtaccgc ctgctcacca
     9421 gcatcataac ggtcaaacaa gcgtataacc ctctaaatag atcagcgggg aacgcaggat
     9481 cgctggctcc ccgtgtaaaa aaaacagccc gaaaccttat tcggtttctt cgctgtcctg
     9541 ttgcgcggaa gtattctgcg cgctgctacc atgatggtag ttactgctgg taccgccacc
     9601 ggcgttgtta ctgtgatgag aaaccgggcg agacgggaca acagtagaaa tcgcgtgctt
     9661 gtaaaccatc tggctgaccg tgtttttcaa caggatcacg aattgatcaa aagactcgat
     9721 ttgcccttgc agcttaatac cattcaccaa ataaatagaa actggaacac gttcccgacg
     9781 cagtgcgttc aggaacggat cttgtaaaga ttgcccctta gccattctct cttttcctta
     9841 tatgcttatt tgtactttga acctttcgat tctgaaaaaa ttgcgcacga tacgtctcaa
     9901 ttgtacacat tcagcctgcg atagcaccaa caacctgtaa tacttcgtcg cgcgcctgtt
     9961 ctggtttttc actgtcaagc cagtgaaccc cttcccaacc acgcagccag gttatctgcc
    10021 gcttcgccaa ctgtctcgtg gcgcaaacac ctcgataaac catttcatcg tatgagattt
    10081 cgccttcaag gtaagaccac atctggcgat aacccacgca acgaatggaa ggcaagtccg
    10141 tatgcaaatc tcctcgggca aaaagcgccc ggacttctgc ttcaaaacct gaagccaaca
    10201 tctgatgaaa acgctgctca atgcgttgat ggagcagttc acggctcgcc ggggcgatgg
    10261 cgaactgatg cacctgatac ggtagagcgt ctcctgacgt ttgcgtcagt tccgttaaag
    10321 ttttacccga aatgaaaaaa acttccagtg cccgggaaag cctttgtgga tcatttggat
    10381 gaatccttgc tgccgcaacc ggatctacct cctgaagttg acgatgcaat gactcccaac
    10441 cttgctctac cgcctgttgc tcaattctgg cccgcacttc cgggtctgcc gacggtagcg
    10501 gcgacaaccc ttccagcaat gccttgaaat acaacatcgt accgcccact aacagtggga
    10561 tccgccccgc cgcggtgata tcggccattt ccgccagcgc atcgcggcga aaatcagcag
    10621 ccgagtaagc ctgcgacgga tcgcgaatat ccagcaatcg gtgcggcgcg gcgagtaact
    10681 cttcagcgtt cggcttcgcc gtcccgatat ccatcccttt gtaaataagg gcagaatcaa
    10741 cgcttatcaa ctctactggt aaaattttac gcagctcaat ggctaacgcc gttttaccgg
    10801 aggccgtcgg ccccatcaaa aaaatcgcct taggcaggct cgccttactg atatcactca
    10861 tctttcaggg cttttatcgc cggatgtaaa tcaacagatt gtaacagacc acccggcggc
    10921 gttttcacaa gttgcggaca taaccgttcc acgtccgcca gcagggttat ggcctgtgcc
    10981 attgaccact gcgcatgttc gctcatcaga tttcgtgcaa tccactgcgc aatattgcca
    11041 ggttcgaata cggactgctt cgccaggtag cctatcagtt caggaatcaa gatttgtaaa
    11101 ttttgttggc gtaagggtaa aggcacggcc ctgatggtca catgctgtgc atctgactgg
    11161 aaatcaatac ccaattccgc cagggcagac tgcgcttttt ctaatgccga tttttcttcg
    11221 ccagaaactt ttagccgcaa cggaatcagc aacggctggg cgcaaactgg cgcttcaccc
    11281 ggcgtcagtt gcgcctgacg cagccaacgt tctgccactg gcaaggctaa aagtgaaatg
    11341 ttgccatcgc gctccagcaa cgcacagtcg gaatggacga tagtcagtac ccgaccaaaa
    11401 ctctgactgt tcgccgcaag tgcaggttcc tgcggttccg gcgcttttgg tttttgcatc
    11461 ggcgcgggcg tttgcaaaag ctggcgatac acttcacctt gctgtttctg gtagcctggc
    11521 tgcgcattcg gccagggggc agccggacga ctgcctgagg cgggcgcagg agagtagcgc
    11581 ggagctaccg gctcacgagc tgccggttct gcaaagtgat tgcgccccgc cgccacgcgg
    11641 ttttccggaa tgggacgcgg tgcaggttgg ggttcatcgt ccagcggtag cggcgtttcc
    11701 agttgctgtt gtagcacgct cagcacgccc tgatagataa aatcatgcac cagacgcgac
    11761 tgatggaaac gcacttcgtg tttggcgggg tgtacgttga cgtccacctg atgcgggtcg
    11821 atctccagat acaacacaaa cgccggttgt tgatcggccc ccagtttgtc ttcgcaggcc
    11881 tggcggatcg catgattgat caggcgatcg cgcatcatgc gaccattcac gtagcaatac
    11941 tgaatttccg ccagtgcggg cgtagtgtga tttggatctg ccacccagcc gcgcagcgtg
    12001 agatcgccgt gttgccattc aatcgccagc gcttgttcga gaaaagcggt gccacaaatc
    12061 gcgcctaagc gccgttcttt ttgcccgcct tccggcactg cgcggtactg acgcacaatt
    12121 ttaccgttat gcgacaggtt gatcgtgacg tcgaaacgcg ccagcgcaat gcggcggatg
    12181 atttcatcaa tatggctaaa ttcggttttc tcggtgcgca ggaatttgcg ccgcgccggg
    12241 gtgttgtaga acagatccag cacctccagc gtcgtcccca caggatgcgc cgccggtttt
    12301 accgtcacgt tcatatcgcg cccttcggca taggcctgcc aggcttcctg ctgttctgcg
    12361 gtgcgtgaag tgagcgtcag gcgggaaacc gaactgatac tcgccagcgc ctcaccgcga
    12421 aagcccaggc tgataatggc ttcgagatcg tccagagagg cgattttact ggtggcatga
    12481 cgagccagcg ccagcgccag ctcatctttt ttgataccgc agccgttatc acgaatgcgg
    12541 ataagtttcg ccccaccgcg ttcgatatca atatcgatac gcgtcgcacc tgcatcgagg
    12601 ctgttttcca ccagttcttt gactaccgac gcaggtcgct cgaccacctc acctgcggca
    12661 atctggttcg ccagttgtgg cggtaagacc tgaattggca tcaatcgtcc ttagtttggc
    12721 agcgtgcgat ctggcgtcgt caccgtactg gcagtttgtg ccgttgcccc ctgcggcgca
    12781 gattgcatcg gatgcgcaag gaaataattg cgcaggcctt tgtaaatggc ttctgccagc
    12841 tgttgttggt aatcgtcgct cgccagcaaa cgttcttcgc tgttgttgct gataaaaccg
    12901 gtttcgacca gtactgacgg gatatccggc gaacgcagaa cgccgagact cgcgtgttct
    12961 ggtcggcgtt tatgtatttc gccaatgcgt tgcaactgac tgatcatact ggtcgctaca
    13021 tcatacccta cccgttggga atgaccgaac tgtaaatcca gcaccgcctg gcttaaatag
    13081 gggtcagact gactgttcgc cagcacatca cccgccccac ccagcagctc cgactgtttc
    13141 tcgtgctgct ccagccagct ggccatttcg ctgttggcgc gacggttaga gagcacccat
    13201 acggaagcgc cagtcgcact gcggttcggt gcggcatcag cgtgaatcga cacgaggaaa
    13261 ttggcgtttt gcttacgtgc cacatcactg cgccccatca ccgagataaa gtaatccccg
    13321 tcacgggtta aaacgccctt aaacatcgga tcgtcattga gcaaagtacg caatttacgc
    13381 gcgatggcga tggtgacatt tttctcccgc gtaccaccgg ggccgatagc gccagggtcc
    13441 tgaccgccgt gtccggcatc aatagcgatg ataattttat cgccagtgtt agccgtcgcg
    13501 cgcgctgccg gacgcgttac cgtattactg ctgataacac ccgtagtgcg gttactttcc
    13561 gttttaaacg gattgcgcgc cggttcgctg acgcgcggtg cgacaaccgc aggcgtttca
    13621 acgcgtttcg caaccacggg cggcggagga ggaggtggcg gcgcatcggc gttaatcgta
    13681 aagacgacag tgtaattgct gccattctgc cgcttcaccg cttcggtttt accgttttcg
    13741 gtaagatcga ccaccagccg tagcgtttgt gcatctttag gcgttccaga gcgaatcgcc
    13801 ttcaccagat tattgccgct gaacaacaac ggcagtccct gaatcacgcc cgtttgtttg
    13861 atatcgagcg ccacggtgcg tttgctttga tggctaaacg cataatcagg atcgccaata
    13921 aaactcaacg ttatccgcgc ctgttgatta ccgttagaaa cctgaatatc agagagcgtc
    13981 gcggcaccca ccggcgtgca cagcagcagc agcagcgtcg ctaccaacca atttctgatg
    14041 cgatacatca tcccgccacc tttcaaaggt taaccggcta aacgcgccag caacaattca
    14101 cccgcagagg aaaccgcact cacgcgcgcc tcacggcctt gtgcctgata atcaatgtgt
    14161 atttcgacat ccgggtcagg aagaacacct gtaccttgtt gtggccactc caccaggcag
    14221 atggcatcgt tggcaaaata atcgcggatc cccataaact ccagctcctc gggatcggca
    14281 aggcggtaca aatcaaagtg ataaaccatt aaattgtcga gcgtataggg ttcgaccagc
    14341 gtataagtgg ggcttttgac attaccctga tgacccagag cttgtaaaaa gccccggcta
    14401 aaggtggttt tacctgcgcc taaatcgcca tacagataga ttacggttgc gccatcgcag
    14461 gctttcgcta cccgctcgcc caggtctaat gttgcctgct catcagggag cggaattact
    14521 cgattcatca tggtttttat cagtcacttc cgggttaaca atacgctgta gcgtggaaaa
    14581 gagatcggtt gccagcatcc cgcgcgttcc aaaacgcgcc gccagtacgt cagctgcagc
    14641 accgtgcgcg acacagcccg cacaggcggc atcatacggc gacagttttt gcccaagcaa
    14701 tgcgccaata ataccagaga gcacatcgcc catgccgccg ctcgccatgc ctgcatttcc
    14761 ggcatcaata atgcctaaag cgtcaggatg ggcggcgacc acggttccgg cacctttcag
    14821 caccgctacg ccgccataac gttgtaccag acgtttggcg caatgtaagc ggtcactttc
    14881 aatttcagcg acggaacagc ctaacaaccg tgcggcctcg ccaggatgcg gcgtgatcac
    14941 gcgattgtga cgcttatcgg gattgattgc cagcaggttc aatgcatcgg catcccacaa
    15001 catcggtttg cgaaaattct caactttttg cagtgctttt ttcccccact cttgctggcc
    15061 cagaccggga ccaatcacca ccacatcggc ccattccagg ctttcggtaa gagagtccat
    15121 cgtcagttca tgcaccatca attccggtcg tgcagtcagc agcggcgcaa tgttttcact
    15181 gcgggtcagt actcggacta aaccagcacc agcacgtagc gccgcttccc ccgtcatacg
    15241 aatagccccc gccgtgccgt gatcgccacc gataattacc agccgcccgt gatcgccttt
    15301 atgcgaagtc gggcgacgcg gttttagcca gtgagaaagt tgttctgccg aaaaccgctg
    15361 aattttcgtc tcctgacctg ccagccaact atccagcccc agtgagtcaa aatgcagttg
    15421 tccggtaaca tcccgcgctt ttccagtgag caagcctggt ttcagcgcaa taaaagtgat
    15481 ggtgtgatcg gcgttgatca ctgcgcctgg cgtagcgccg gtttcagcca gcaggccgga
    15541 agggatatca accgccgcaa tcggcgcagg atgggaatta gcgtggtcga ttaactggct
    15601 aatggattcg cggggcgctt gctgcaagcc ggtaccgagc agcgcatcaa caatcagatc
    15661 taccgattcg ggccagacaa tattcgaagc atggatctcg cctcccgcgt ttaaccatgc
    15721 ttcgcgtgcc agcgcggcct cttccggcaa cggtttgtcg ctctcctggg ccaacaacgt
    15781 gacctcaatg ccgaccgctg tggccagtcg cgcgaccacg tagccatcgc cgccgttatt
    15841 accatgaccg cacagcacca gccagtggcg ggcgtcagga tacgccgaac gacacacctg
    15901 gaatgcggct tcgccagcgc gaagcatcag ctcatagagt gtaagcccca gcgcatctgc
    15961 cgcctcacgt tctccgcggc ggatatcgtc ggcgtgccag acggtgtgtg gtatacttac
    16021 ggggtttttc ttcattgtat ggtccgtcat gtcagagccc ctcgatctca atcagttagc
    16081 gcaaaaaatt aaacagtggg ggctggaact gggctttcag caggtaggta ttaccgatac
    16141 cgatctcagc gagtccgagc ccaaactgca agcatggctg gacaaacaat accacggcga
    16201 aatggactgg atggcacgtc acggtatgct gcgcgctcgc ccccatgagt tattacccgg
    16261 tacgctgcgc gtgatcagcg tgcggatgaa ttaccttcct gctaacgccg catttgccag
    16321 cacgctgaaa aaccccaaac tcggctatgt tagccgttat gcgctgggcc gtgactatca
    16381 caaacttctg cgcaaccgac tcaaaaagct gggcgagatg attcagcaac attgtgtttc
    16441 gctgaatttt agaccgtttg tcgattctgc gcctattctc gagcgcccgt tagctgcaaa
    16501 agctgggctc ggctggacag gtaagcactc acttatcctc aatcgcgagg ccggttcttt
    16561 cttcttttta ggcgaattgc tggtcgatat tccgctgccc gtggatcaac cagtcgagga
    16621 aggatgcggt aaatgcatag cctgtatgac gatttgcccg accggtgcca tcgtcgagcc
    16681 atataccgtc gatgctcgcc gctgtatctc ttatctcacc atcgaactgg aaggggcgat
    16741 cccggaagag ttgcgaccgt taatgggaaa ccgtatttac ggttgcgatg actgccagct
    16801 tatctgcccg tggaatcgct attcgcaact cactacagaa gacgatttca gcccgcgtaa
    16861 gccgctacac gcaccggaac tcattgagtt attcgcctgg agcgaagaga agtttttaaa
    16921 agtcacggaa ggatcggcga ttcgccgtat aggtcacctg cgctggctgc gtaatatcgc
    16981 cgtggcatta ggcaatgccc cctgggatga aacgattctg gcggcgctgg agagtcgcaa
    17041 aggtgagcac ccacttcttg atgagcacat agcgtgggcg atggcgcagc aaatcgagag
    17101 gcgaaatgcg tgcatagtcg aagtgcaatt gccgaaaaaa cagcgtctgg ttcgggtgat
    17161 tgaaaaaggg ttaccacgtg acgcctgatt catccacagc ttgtgaataa aaataaaaac
    17221 gcattgcaat tcaagacgaa taaaatcgtc aagtgatcga ctcgacaaaa cgaaatctta
    17281 atttttaact tatctatcaa taagttaaaa aaatactatt caccgtgcga agtttctttg
    17341 aaattaagac tacagcctga ggctgtggat aactctgttt acaagagttt gtcagagaca
    17401 acaaaaacca gcaataacgc atcgcgtcgc tgtggatatt ttattgagag aagaatttgg
    17461 agcgggaaac gagactcgaa ctcgcgaccc cgaccttggc aaggtcgtgc tctaccaact
    17521 gagctattcc cgcttgggtg gtctgtgcct tacagcactt tcaaattttg gagcgggaaa
    17581 cgagactcga actcgcgacc ccgaccttgg caaggtcgtg ctctaccaac tgagctattc
    17641 ccgcttaatc ttcgtctttc gaaccgctac tgccttggct gccttcactc accttagtca
    17701 catacttttg taagttccca aggattcgtt cagttgccgc cttgtcgcaa ttcgaaatac
    17761 tgcgattggg tggtctgtgc tttgcagcac ttttcaaatt ttggagcggg aaacgagact
    17821 cgaactcgcg accccgacct tggcaaggtc gtgctctacc aactgagcta ttcccgcgta
    17881 ctacttaatt ttgctttcgt aattttcgca tttctgcgtc gttacgggag gcgcattata
    17941 cgagaaatct ttccctctgc aaccccccaa aaaagttttt tttgcgtttt tatctcaagt
    18001 gctgaaatta tcagcaagac gttcaattta gcgtcaaaat cacgccgccg caagtggatt
    18061 ccggcgctta cgtgaccagg aaaaatttta cagcttgata aaatgctcgc ggtagtaagc
    18121 cagctccgcc accgattcac ggatatcatc catcgcctga tgcgtcccct gcttggtaaa
    18181 accatccaga atttccggct tccagcggcg cgccagctct ttcagggtgc tgacatcgag
    18241 ataacggtag tggaagtagg cttccagctc cggcatgtat ttaaacagga aacgacggtc
    18301 ctgaccgatg ctgttaccgc aaatcggcga ttttcccgca ggcacccact gttttaaaaa
    18361 ttcgagcgtt gccagttcag cttcccgatc gcccatcgtg ctcgctttca cgcgctctac
    18421 cagcccgctg gcggtatggg tgcgcacgtt ccagtcatcc atcagcgcca gctgttcatc
    18481 agactggtgt actgcaatgg tcggcccttc tgccagaata ttcaggttgg catcggtcac
    18541 cagcgtggca atctcaataa tgcgatcgcg ctcgggatcc agaccggtca tctcaagatc
    18601 gatccaaatc aggttgtttt cattggcact catgctattt tccacccatt cacagtgact
    18661 atattgatcc acaattgcgt gtatcataga tgttttgccc atcaggggcg accaggagtc
    18721 tgtacgattg agtaaaaata aactctccaa aggccagcag cgccgcgtga acgccaatca
    18781 ccagcgtcgt cttaaaacgt ctaaggagaa gcccgactac gacgacaatc tgtttggcga
    18841 gcctgatgaa ggtatcgtca tcagccgctt tggtatgcac gctgatgtgg aatccgccga
    18901 tggcgacgtt caccgctgca atattcgccg taccatccgt tcgctggtaa ccggcgaccg
    18961 cgtagtctgg cgtccgggta aaccggcggc ggaaggcgta aatgtcaaag ggatcgtgga
    19021 agcggtgcat gagcgtacct cggtgttgac gcgtccggat ttctacgacg gcgtaaaacc
    19081 tattgccgcc aacatcgacc agattgtcat tgtctccgcc attttgccgg agctgtcgct
    19141 caatattatc gaccgttacc tggtggcctg cgaaaccttg cagattgagc cgattattgt
    19201 gctcaacaag atagacctgc tggacgacga aggcatggcg ttcgtcaacg agcagatgga
    19261 tatctaccgc aatatcggtt atcgcgtatt gatggtttcc agccatactc aggatgggct
    19321 aaaaccgctg gaagaggcgt tgaccgggcg catcagcatt tttgccgggc agtctggcgt
    19381 cggcaaatcc agcctgctga atgcgttgct ggggctgcaa aaagagatcc tgaccaacga
    19441 tgtctctgac aactcggggc tcggtcagca caccacgacc gccgctcggc tgtatcactt
    19501 cccgcacggc ggtgatgtga ttgattcccc tggtgtgcgt gagttcggcc tctggcacct
    19561 ggagccggaa caaatcactc agggctttgt cgaattccat gactatttag gtctgtgtaa
    19621 atatcgcgat tgcaaacacg ataccgatcc gggctgcgct atccgggaag cggttgagga
    19681 agggaaaatc gcggaaaccc gtttcgaaaa ctatcaccgt attctggaaa gcatggcgca
    19741 ggtaaaaacg cgtaaaaact tttctgatac ggatgactga caattaagct aaccgtcgtt
    19801 agaatcgtcc cctttttttc aggatccggc atgttggccg gatcaggaac gacaaaacaa
    19861 tggcctggag gctaccttgt taaattcatt taaactttcg ctacagtaca ttctgccgaa
    19921 actatggctt actcgcctgg cgggttgggg cgcaagcaag cgggcaggat ggctgacaaa
    19981 actggttatc gatctgttcg ttaaatacta caaggtcgac atgaaagagg cgcaaaagcc
    20041 ggacaccgcc agctaccgca cctttaacga attctttgtc cgtccgctgc gtgacgaagt
    20101 acgcccaatc gataccgatc cgaatgtcct ggtcatgcct gccgatggcg ttatcagcca
    20161 gttgggtaaa atcgaagaag ataaaatcct gcaagccaaa ggccacaact acagcctcga
    20221 agccctgctg gcaggcaact atctgatggc ggacctgttc cgcaacggta cgtttgtgac
    20281 cacttacctc tccccgcgtg actaccatcg cgtacacatg ccgtgcaacg gtattctgcg
    20341 tgagatgatc tacgtgccgg gcgatctctt ctccgttaac catctcaccg ctcagaacgt
    20401 gccgaatctg tttgcccgta acgaacgcgt gatttgcctg ttcgataccg aatttggccc
    20461 gatggcgcag attctggtcg gggcgacgat tgtaggcagc attgagacgg tctgggcggg
    20521 caccattacg ccgccgcgcg aaggtatcat caagcgctgg acctggcctg ccggggaaaa
    20581 cgacggttct gtggcactgc tgaaaggcca ggaaatgggc cgctttaaac tcggctctac
    20641 cgttatcaac ctgtttgcac cgggtaaagt gaatctggtt gagcaactgg aaagcctgtc
    20701 tgtcacgaaa attggccagc cgctggcagt atctaccgaa acctttgtta cgccagacgc
    20761 tgaacccgcc ccacttcctg ctgaagagat cgaagcagaa cacgacgcca gcccattggt
    20821 tgacgataaa aaagaccagg tctaatccat caaaggaaac gctgacgtgc gcctgattat
    20881 cacttttctg atggcctggt gcctcagttg gggggcgtac gccgcgacgg cccccgatag
    20941 caaacaaatc actcaggaac tggagcaggc aaaagcggcg aaacccgcac agccggaagt
    21001 cgtagaggcg ctccagtctg ccttaaatgc gcttgaggaa cgaaaaggtt cccttgagcg
    21061 catcaaacaa tatcagcaag ttatcgataa ttatccgaaa ctctccgcta ctctgcgcgc
    21121 acaattaaac aacatgcgtg acgagccgcg cagcgtgtcg ccgggaatgt ctaccgacgc
    21181 gctgaatcag gaaattctcc aggtcagcag ccagttgctg gataaaagcc gtcaggccca
    21241 gcaagagcag gagcgcgccc gcgagattgc cgattcgctg aatcaactgc cgcaacagca
    21301 aaccgacgcc cgccgtcagt taaatgagat cgagcgccgc ctgggaacgc ttactggcaa
    21361 taccccgctc aatcaggcac aaaatttcgc gttgcagtct gactccgcac gcctgaaagc
    21421 gctcgttgat gaactggaac tggcgcagct gtctgccaat aaccgtcagg aattagcgcg
    21481 cttacgctca gagctggcgg aaaaagagag ccagcaactg gatgcgtatt tgcaggcctt
    21541 acgtaatcag ttgaacagcc aacgtcagct tgaggcggag cgggcgctgg aaagtaccga
    21601 attgctggca gaaaacagcg ccgatttgcc gaaagacatc gtcgcgcaat tcaaaattaa
    21661 ccgcgaacta tcggcggcac tgaatcaaca ggcgcagcgg atggatctcg ttgcctcgca
    21721 acagcgccag gccaccagcc agacgttaca ggttcggcag gcgctgaata cgctgcgtga
    21781 acagtcgcaa tggctgggat cgtccaacct gctcggcgaa gcgttgcggg cgcaggtggc
    21841 acggctgccg gaaatgccga aaccacaaca gcttgatacc gaaatggcgc agttgcgtgt
    21901 gcaacggtta cgttatgagg atctgcttaa taaacagccg ctgctacggc aaattcatca
    21961 ggccgacggt cagccgctga ccgccgagca aaaccgtatt ctggaagcac agctacgcac
    22021 tcagcgtgag ttgctgaact cattgttgca gggtggcgac acgctactgc tggaactgac
    22081 caagctgaaa gtctccaacg ggcaacttga ggatgcgctg aaagaggtga atgaagcgac
    22141 gcatcgctat ctgttctgga cctctgacgt gcgcccgatg accatcgcct ggccactgga
    22201 aatcgcccag gatctgcgtc gtctcatttc gctggacacc ttcagtcagt tgggcaaagc
    22261 cagtgtgatg atgctgacca gcaaagagac gattttgccg ctgtttggcg cgttgattct
    22321 ggtcggttgc agtatttact cgcgccgcta tttcacccgt tttcttgaac gttcggcggc
    22381 gaaagtcggc aaagtgactc aggatcactt ctggctgacg ttgcgcactc ttttctggtc
    22441 gattctcgtc gcgtcaccgc tgccggtgct gtggatgacg ctgggttacg gcttgcgcga
    22501 ggcgtggcct tatccgctgg cggtcgcgat tggcgatggc gtaacggcca ccgtgccgct
    22561 gctgtgggta gtgatgattt gcgccacctt tgcccgcccg aacggcttgt ttatcgctca
    22621 ttttggctgg ccgcgcgaac gtgtttcccg tgggatgcgc tactacctga tgagcatcgg
    22681 gcttattgtg ccgctgatta tggcgctgat gatgttcgat aacctcgacg accgtgaatt
    22741 ctccggttcg ctgggacggc tttgctttat cctcatttgc ggtgcgctgg cggtggtcac
    22801 cctcagcctg aaaaaggccg ggatcccgct gtacctcaac aaagagggca gcggcgacaa
    22861 cattaccaac catatgctgt ggaacatgat gattggcgca ccactggttg ccattctggc
    22921 gtcagcggtg ggttatctgg caacggcgca ggcactgtta gcgaggcttg aaacctcggt
    22981 tgccatctgg ttcctgctac tggtggttta tcacgttatc cgccgctgga tgctgatcca
    23041 gcgtcgcagg ctggcgtttg accgggcgaa gcatcgccgg gcagagatgt tagcgcaacg
    23101 cgcgcgtggc gaagaggaag cgcatcatca cagtagcccg gaaggggcaa ttgaagtcga
    23161 tgaaagcgaa gtcgatctcg atgccatcag tgcgcaatcc ttgcggctgg tgcgctcaat
    23221 tttgatgttg atcgccctgc tttctgtcat tgtgctgtgg tcagaaatcc attccgcttt
    23281 cggcttcctc gaaaatattt cgctgtggga tgtcacctcc acggtacagg gcgtagaaag
    23341 tctggagcca attaccctcg gtgcggtgct gattgccatt ctggtgttta tcatcaccac
    23401 gcagctggtg cgcaacctgc ccgcgctgct ggaactggcg attttgcagc acctggattt
    23461 aacgccgggt acgggctacg ccatcaccac catcaccaaa tatctgctga tgctgattgg
    23521 cgggctggtc ggcttctcga tgattggtat tgagtggtcg aaattgcagt ggctggttgc
    23581 cgcgctcggt gttggtctcg gttttggttt gcaggagatt ttcgccaact ttatctctgg
    23641 cctgattatc ctgttcgaaa aaccgattcg cattggcgat acggtaacga tccgcgatct
    23701 taccggtagc gtgacgaaaa ttaacacccg cgccaccacc atcagcgact gggaccgtaa
    23761 agagataatc gtgccgaaca aggcgtttat taccgagcag tttatcaact ggtcgctctc
    23821 tgactcggtc acgcgcgtgg tgttgacgat accggcccct gccgatgcca atagcgaaga
    23881 agtgacggaa atcctgctca ccgcagcgcg tcgctgctcg ctggtgatcg acaacccggc
    23941 accggaagtc ttcctggtgg atctgcaaca ggggattcag attttcgagc tgcgtattta
    24001 cgccgctgag atgggtcacc gtatgccgct acgccatgag atccaccagc tgattctggc
    24061 aggcttccac gcccacggta tcgatatgcc attcccgccc ttccagatgc gtctggaaag
    24121 cctcaacggt aaacaaacgg ggagaacgct gacgtctgcg ggcaaaggtc gtcaggcggg
    24181 aagtttgtaa gtttgtgtgc cggatgaacc aatccggctc acaaacaaaa ctgatagcgc
    24241 agaaaaaact gccacagcca gtagccgaaa atagccagcg cggtggcgta cataaaccag
    24301 ccgcgttttt ttcgccacaa agcgaaccag agaaaaggga aaaagagcgg taacgtcatt
    24361 atcacaccaa catcacgggt atcgtcttct tcgagataca tcaacacatc gcagacgttg
    24421 ttaatcccgc gtccatcaat catccactct ttgtccagcg cggaagtgat ggttatccag
    24481 aggaacgcca caataaacca gatgcagcat aagacaaaca tgcgcgcact cataaggtaa
    24541 ccggtcgctt aatttcgcta tagcgcatac gaaacaaatc gccgtatccc actaattccc
    24601 cgcgaaaaac cagacatcgg ataagataag cgcaataaat atccggcagg tttccccacc
    24661 gtttaccatc gcgatctttg agggtgttaa tggtgctacc caccaccatc gccactttac
    24721 gggtgtggca ggcatcgaca aaagagagaa tcgtctgctc tatttccgct acatccccgg
    24781 cagaaaattg cagcgccctg atttcatctt cggttattgc cgggtcccgg ctggtgtcat
    24841 ccataacatt tcctgtcttt acgccctatc gtcaggtaga cgatagggca tcgggttatt
    24901 ccaccgccca ctgcggattt tttgccactc tgctgcaata gttctggtaa atcgccatcg
    24961 ctaacagcga gaagaagaca gggccgccaa tcatccacaa tgtgctgtcc cagtctccgg
    25021 cttcaaccac aggttgaatg atggtgaaga cgttcgcaaa tgtcaccacc agtacgacaa
    25081 ccactgttgc aatcattgcc gacatatgcg ttttgaaaat cacaaatggt ctgtcgagat
    25141 cctgacgtgc tttaaaaaat gggaaagcca gcgcgaggaa caggtaagga agcgtcatag
    25201 acacgttcgc catcagcgtc agcttgttaa agaacgccga tgcggtaccg ccaccaaacg
    25261 aaaccagcag gatgaagaca gtaaccaacc cgcactgcat ccacatggcg atagacggca
    25321 tccccatcgc attcaggcgc gtcatcggtt ccggccacaa cgctttcggc gtcccctgga
    25381 tgatggcttt cagcggtgaa tagcaaagcg taaagaacgc accggtataa gcgaggaaca
    25441 tcgaaagtcc ggtaatacgc gcaaaccata cgcccagcga caatgacgct tcaggtgaca
    25501 aatgcagtgc gttacccagc gtcaccccga ggctcttcat cagcacatag gtaatattgc
    25561 caaggttaac ggaaccatta cttaatacct gctgccagtt tgtgctgagg ccccataaaa
    25621 atattgccag cgaataaccg attgaaataa caatagcggc aaaaacaata cctttggcaa
    25681 agttcttttc tggattttcc gttttatcga ccagaccacc aaccgcttca attccgccat
    25741 aggcaaaaat agcaaatacc acaaacgata gcattgccag accggactga taacctgggt
    25801 tcggtgatgc aaggaaatta atatcctgcg cgaaatgccc accatttaat aacaaaatag
    25861 taatgcttac taacagcaat actaaattca gacacattac tgcaataccg cccaccgcag
    25921 taatgcgggc aattttatta atcccctttg aagcaacgac ggtgaccaga atcatccatg
    25981 ccactgccag cagaccaacc acctgcgtag gctccagtcc agcaatacgc cagtgctggg
    26041 tcatgtcgct accatagagg aatgttgaga acggtaccca aactttcgcg gaggtactca
    26101 ccatccagat gatataagag gaaaaccaca taaacgtacc aatgaaggca aaacgtggtc
    26161 cgacactatt attcatccag gaatagatac cgccttcttc tttgcgataa gcagctccca
    26221 tttcagccat cattaaggcg aatggaataa agaataacaa tgcagaaaat atataaaagg
    26281 gaatcgcact ataacccatt aagtaataag ccgatgggct attggcaaat ccaaatacgg
    26341 aagtaaagat catcaatatg agtcctatca gactcatctt ttttatcgtg tgaggcatga
    26401 aaccatcctt catttcagcg cgtgtataac acgtgcccac tgaactgcac tgcgcaacat
    26461 tgagcaggca gcgtaaaaac caatacagaa ttgatacctg gatgatatca aatatccagt
    26521 cttaaattgt ggcctttaac ggaaaaatga aaacatatgc tacagaatgg cgcttatcac
    26581 gccattcttc gctgttaatt cagtaatttt tcagaattat gcccggtcaa cgctaaaggc
    26641 gatgacttca gccagtgtct ccgcgcccag cgccaacatc accagacgat caacacctaa
    26701 tgccacgccg gaacagtcag gcataccgac tttcaaggct tcaatcagat tctggtcaat
    26761 ggggtgctgc ggcaaaccgc gcgccgcgcg cttacggtta tcttgttcaa agcgttgttg
    26821 ctgctcacgg gcatccgtca attcatggaa accattcgcc agctcaatac ctttataata
    26881 aacctcaaag cgttcagcga cccgatgatc ttcggtactg atttgcgcca gtgatgcctg
    26941 gctggctgga aagtggtaca caaaggtcgg tttttctttg ccaatatttg gctctacgcc
    27001 aaaggtaaac agcaattgta gcagcgtgtc gcggtcttct tcggtatcag caacattgct
    27061 caaatccagt ttcgctgcga cttcccgcag ttgcgttttg tcggcagaga gcgggtcaat
    27121 ttccagataa cgcaagaaag cttgttgata agaaaggctt tctgctgccg ggcagtccag
    27181 cacctgttgt aagagatcgt ccacctcgtt catcaaccgg tacatatcat agtgcggtcg
    27241 ataccactcc agcatagtga actcagggtt gtgataacgc cccatctctt cattacggaa
    27301 gctgcggcac agctggaata ccggcccaca accggcaacc agcaggcgtt tcatatggta
    27361 ttccgggctg gtcattaacc agagattcat cccctgcgaa tgcccggggc caacgaaacg
    27421 tgtctcaaac gggaccaaat gaatatcggt taccgtcgcc tggctcatac aaggcgtctc
    27481 cacctccagc actccacgat cggcaaagaa acgacggatc tccgccataa tcgccgcgcg
    27541 ttttaataag ttaggaatgg atgcgctcgg ctgccaggat gccgtttcgc tcatagttaa
    27601 atctccagtt tttgacaagg gcacgaagtc tactcgcaac gcgacggcga gacaaatttt
    27661 acgcaggaat caaacagcgg tgggcagtga ctaaaaaaag cacgatctga tggtttagta
    27721 attaaattaa tcatcttcag tgataattta gccctcttgc gcactaaaaa aatcgatctc
    27781 gtcaaatttc agacttatcc atcagactat actgttgtac ctataaagga gcagtggaat
    27841 agcgttcgca gaccgtaact ttcaggcact taccctgaag tacggggctg tgggataaaa
    27901 acaatctgga ggaatgtcgt gcaaaccttt caagccgatc ttgccattgt aggcgccggt
    27961 ggcgcgggat tacgtgctgc aattgctgcc gcgcaggcaa atccaaatgc aaaaatcgca
    28021 ctaatctcaa aagtataccc gatgcgtagc cataccgttg ctgcagaagg gggctccgcc
    28081 gctgtcgcgc aggatcatga cagcttcgaa tatcactttc acgatacagt agcgggtggc
    28141 gactggttgt gtgagcagga tgtcgtggat tatttcgtcc accactgccc aaccgaaatg
    28201 acccaactgg aactgtgggg gtgcccatgg agccgtcgcc cggatggtag cgtcaacgta
    28261 cgtcgcttcg gcggcatgaa aatcgagcgc acctggttcg ccgccgataa gaccggcttc
    28321 catatgctgc acacgctgtt ccagacctct ctgcaattcc cgcagatcca gcgttttgac
    28381 gaacatttcg tgctggatat tctggttgat gatggtcatg ttcgcggcgt ggtagcaatg
    28441 aacatgatgg aaggcacgct ggtgcagatc cgtgctaacg cggtcgttat ggctactggc
    28501 ggtgcgggtc gcgtttatcg ttacaacacc aacggcggca tcgttaccgg tgacggtatg
    28561 ggtatggcgc taagccacgg cgttccgctg cgtgacatgg aattcgttca gtatcaccca
    28621 accggtctgc caggttccgg tatcctgatg accgaaggct gccgcggtga aggcggtatt
    28681 ctggtcaaca aaaatggcta ccgttatctg caagattacg gcatgggccc ggaaactccg
    28741 ctgggcgagc cgaaaaacaa atatatggaa ctgggtccac gcgacaaagt ttctcaggcc
    28801 ttctggcacg aatggcgtaa aggcaacacc atctccacgc cgcgtggcga tgtggtttat
    28861 ctcgacctgc gtcacctcgg cgagaaaaaa ctgcatgaac gtctgccgtt catctgcgaa
    28921 ctggcgaaag cgtacgttgg cgtcgatccg gttaaagaac cgattccggt acgtccgacc
    28981 gcacactaca ccatgggcgg tatcgaaacc gatcagaact gtgaaacccg cattaaaggt
    29041 ctgttcgccg tgggtgaatg ttcctctgtt ggtctgcacg gtgcaaaccg tctgggttct
    29101 aactccctgg cggaactggt ggtcttcggc cgtctggccg gtgaacaagc gacagagcgt
    29161 gcagcaactg ccggtaatgg caacgaagcg gcaattgaag cgcaggcagc tggcgttgaa
    29221 caacgtctga aagatctggt taaccaggat ggcggcgaaa actgggcgaa gatccgcgac
    29281 gaaatgggcc tggcaatgga agaaggttgc ggtatctacc gtacgccgga actgatgcag
    29341 aaaaccatcg acaagctggc agagctgcag gaacgcttca agcgcgtgcg catcaccgac
    29401 acctccagcg tgttcaacac cgacctgctc tacaccattg aactgggcca cggtctgaac
    29461 gttgctgaat gtatggcgca ctccgcaatg gcacgtaaag agtcccgcgg cgcacaccag
    29521 cgtctggacg aaggttgcac cgagcgtgac gacgtcaact tcctcaaaca caccctcgcc
    29581 ttccgcgatg ctgatggcac gactcgcctg gagtacagcg acgtgaagat tactacgctg
    29641 ccgccagcta aacgcgttta cggtggcgaa gcggatgcag ccgataaggc ggaagcagcc
    29701 aataagaagg agaaggcgaa tggctgagat gaaaaacctg aaaattgagg tggtgcgcta
    29761 taacccggaa gtcgataccg caccgcatag cgcattctat gaagtgcctt atgacgcaac
    29821 tacctcatta ctggatgcgc tgggctacat caaagacaac ctggcaccgg acctgagtta
    29881 ccgctggtcc tgccgtatgg cgatttgtgg ttcctgcggc atgatggtta acaacgtgcc
    29941 aaaactggca tgtaaaacct tcctgcgtga ttacaccgac ggtatgaagg ttgaagcgtt
    30001 agctaacttc ccgattgaac gcgatctggt ggtcgatatg acccacttca tcgaaagtct
    30061 ggaagcgatc aaaccgtaca tcatcggcaa ctcccgcacc gcggatcagg gtactaacat
    30121 ccagaccccg gcgcagatgg caaagtatca ccagttctcc ggttgcatca actgtggtct
    30181 gtgctacgcc gcgtgcccgc agtttggcct gaacccagag ttcatcggtc cggctgccat
    30241 tacgctggcg catcgttata acgaagatag ccgcgaccac ggtaagaagg agcgtatggc
    30301 gcagttgaac agccagaacg gcgtatggag ctgtactttc gtgggctatt gctccgaagt
    30361 ctgcccgaaa cacgtcgatc cggctgcggc cattcagcag ggcaaagtag aaagttcgaa
    30421 agactttctt atcgcgaccc tgaaaccacg ctaaggagtg caacatgacg actaaacgta
    30481 aaccgtatgt acggccaatg acgtccacct ggtggaaaaa attgccgttt tatcgctttt
    30541 acatgctgcg cgaaggcacg gcggttccgg ctgtgtggtt cagcattgaa ctgattttcg
    30601 ggctgtttgc cctgaaaaat ggcccggaag cctgggcggg attcgtcgac tttttacaaa
    30661 acccggttat cgtgatcatt aacctgatca ctctggcggc agctctgctg cacaccaaaa
    30721 cctggtttga actggcaccg aaagcggcca atatcattgt aaaagacgaa aaaatgggac
    30781 cagagccaat tatcaaaagt ctctgggcgg taactgtggt tgccaccatc gtaatcctgt
    30841 ttgttgccct gtactggtaa ggagcctgag atgattaatc caaatccaaa gcgttctgac
    30901 gaaccggtat tctggggcct cttcggggcc ggtggtatgt ggagcgcaat tattgcgccg
    30961 gtgatgatcc tgctggtggg tattctgctg ccactggggc tgtttccggg tgatgcgctg
    31021 agctacgagc gcgttctggc gttcgcgcag agcttcattg gtcgcgtatt cctgttcctg
    31081 atgatcgttc tgccgctgtg gtgtggttta caccgtatgc accacgcgat gcacgatctg
    31141 aaaattcacg tgcctgcggg caaatgggtt ttctacggtc tggctgctat cctgacagtt
    31201 gtcacgctga ttggtatcgt tacaatctaa cgtatcgcca atgtaaatcc ggcccgccta
    31261 tggcgggccg ttttgtatgg aaaccagacc ttatgttcaa aacgacgctc tgcgccttat
    31321 taattaccgc ctcttgctcc acatttgctg cccctcaaca aatcaacgat attgtgcatc
    31381 gcacaattac cccgcttata gagcaacaaa agatccccgg tatggcggtg gcggtaattt
    31441 atcagggtaa accttattac tttacctggg gctatgcgga catcgccaaa aagcagcccg
    31501 tcacacagca aacgttgttt gagttaggtt cggtcagcaa aacatttacg ggcgtgcttg
    31561 gtggcgacgc tattgctcga ggggaaatca agttaagcga tcccacaaca aaatactggc
    31621 ctgaacttac cgctaaacag tggaatggga tcacactatt acatctcgca acctacactg
    31681 ctggcggcct gccattgcag gtgccggatg aggtgaaatc ctcaagcgac ttgctgcgct
    31741 tctatcaaaa ctggcagcct gcatgggcgc caggaacaca acgtctgtat gccaactcca
    31801 gtatcggttt gttcggcgca ctggctgtga agccgtctgg tttgagtttt gagcaggcga
    31861 tgcaaacacg tgtcttccag ccactcaaac tcaaccatac gtggattaat gtaccgcccg
    31921 cagaagaaaa gaattacgcc tggggatatc gcgaaggcaa ggcagttcat gtttcgccag
    31981 gggcgttaga tgctgaaact tatggtgtga agtcgaccat tgaagatatg gcctgctggg
    32041 tacgaagcaa tatgaatccc cgtgatatca acgacaaaac acttcagcaa gggatacaac
    32101 tggcacaatc tcgctactgg caaaccggcg atatgtatca gggcctgggc tgggaaatgc
    32161 tggactggcc ggtaaatcct gacagcatca ttaacggcag tggcaataaa attgcactgg
    32221 cagcacaccc cgtaaaagcg attacgcccc caactcctgc agtacgcgca tcatgggtac
    32281 ataaaacagg ggcaaccggc ggatttggta gctatgtcgc gtttattcca gaaaaagagc
    32341 tgggtatagt gatgctggct aacaaaaact atcccaatcc agcgagagtc gccgccgcct
    32401 ggcagattct caacgctcta cagtaaaatt tcatcgggtc cgaattttcg gaccttttct
    32461 gcgcttttcc ttgctgtcat ctacacttag aaaaaaacca gtaaggaaac atttatgcgc
    32521 ctgctccctc tcgttgccgc agcgacagct gcatttctgg tcgttgcctg cagttctcct
    32581 acgccgccgc gtggcgtaac agtagtgaat aatttcgacg ccaaacgtta tctcggtacc
    32641 tggtatgaga ttgcccgttt tgatcaccgt tttgaacgtg gactggaaaa agtcaccgca
    32701 acatacagcc tgcgtgatga cggcggcctg aatgtcatta ataaaggcta taaccctgac
    32761 agaggaatgt ggcagcagag tgaagggaaa gcgtacttta ccggcgcacc aactcgcgct
    32821 gcgctgaaag tgtcattctt tggtcctttc tatggcggtt ataacgttat tgcactcgat
    32881 cgggaatacc gccatgcgct ggtttgcggc ccggaccgcg actacctgtg gatactctcc
    32941 cgcacgccaa ccatttctga cgaagtgaaa caggagatgc tggcagtcgc gacccgggaa
    33001 gggtttgatg tcagtaagtt tatttgggta cagcagcccg gtagttagtg agtgctgagt
    33061 ttcagaccaa taatccccaa tacgattaac gccagactcg ccaggcgcat cgggttagcg
    33121 gactcaccga gcagcacaat gccggtgatg gccgcgccga ctgcgccaat acccgtccac
    33181 acggcataag ccgtccctac tggtaacgat ttcatcgccc aggcaagtag cgccatactg
    33241 acaatcatcg ccgtcacggt aataacactc ggcgtcaaac gactaaagcc gtgggtatat
    33301 ttcaggccaa cggcccatac cacttccagc agaccagcaa taactaagat aatccaggac
    33361 atatcaggct ccggagaatt ggggccgtcc ccggtgaaaa gaggcgtttg caggtcgtcc
    33421 tgcaaagctg atggttgaac ggatattttg ccaggtggca ggggaatttc aactattacc
    33481 ttagtgacta tcgcagaaaa aacaaacggc acgacatacc tgtcgtaccg ttattgctta
    33541 ttgctattgc tgcgctttcg ttgctgcgcc agaaatcgcg ttaccgccat cagaaatgtc
    33601 ttcaccaacg ccacgcgtgg tgttgcaggc agttaatact gttgaaagca ccagaacaga
    33661 aaagatcgct gcaattgtct tcttcaccat aacgtcttcc ttttaggtaa tgttatttat
    33721 gtttgcctat agcaaattaa taatagacaa catcgccaaa aatgacggaa atctgatgat
    33781 ttttcggaag agaagaaaaa ttagctggca gcgcgagaga tggagttgcc gagatgtttg
    33841 atgtcttcgc cgaaaccgcg agcggtgtta cagcccgtga gcagcgtgct ggcaagcaaa
    33901 acaagaacga taaggcgttt catcatctct gacctgaata agtgatggtg cagcctgcag
    33961 gccgcaccac aaccgcatta cttcacgcga gagacgtatt caccagagcg ggtatccact
    34021 ttgatgactt cgccgatttg tacgaacagc ggaaccttaa ccacagcgcc agtagacagg
    34081 gtagccggtt tgccgccagt acctgcggta tcacctttca ggcccggatc ggtatcaacg
    34141 atttccagtt caacgaagtt cggcggagta acggagatcg gctgaccatt ccacagagtt
    34201 acgatacact ctgcctgatc cagcagccat ttagcgttgt cgccaattgc ttttgcatca
    34261 gcagacagct gctcgaaagt ttcgttgttc atgaagtgcc agaactcacc gtcgttgtac
    34321 aggtaagtca ggttcatatc gacaacatca gcgccttcag cggaatcagt agatttgaag
    34381 gttttttcta cgcgagtacc ggtcagcaga cgacgcagtt taacgcgagc aaatgcctgg
    34441 cctttacccg gttttacgaa ttcactcgct tcaaccgcgt aaggttcgcc gtctaacatg
    34501 attttaagac cagcacgaaa atcgttgcta tagtacgttg ccataaggcc ctctgaaatt
    34561 tgttaattgg tagctaagcc acaaaatggc gcatattgta accctaaata ccccatccag
    34621 agaagattgg ttaacgcaac ttgccgatgt tgtgaccgat cctgatgaac ttctgcgtct
    34681 tttgaatata gacgcggacg aaaaactgtt ggccggacgc agcgccaaaa agctgtttgc
    34741 cctgcgtgtg ccccgctcat ttatcgatcg catggagaaa ggcaatccgg acgatcctct
    34801 tttgcgtcag gtacttacct cgcaagatga gtttgtcgtc gcgtccggat tctccaccga
    34861 cccgctggaa gaacagcaca gcgtagtgcc tggtttgttg cataaatacc acaaccgggc
    34921 gcttttgctg gtcaaaggcg gctgcgcggt aaattgccgc tattgcttcc gtcgccactt
    34981 cccctatgcc gaaaatcagg gcaacaagcg taactggcag actgctcttg agtatgttgc
    35041 tgcgcatccg gaactggacg agatgatttt ctccggcggc gatccgctga tggcgaaaga
    35101 tcacgagctg gactggttgc tcacacaact ggaagccatc ccgcatatca aacgtctgcg
    35161 gattcacagc cgtctgccga ttgtgatccc ggcacgtatc accgaggcgc tggttgaacg
    35221 ctttgcccgt tctacgctgc aaatcttgct ggtgaatcac atcaatcatg ccaatgaggt
    35281 agatgaaacg ttccgtcagg cgatggctaa attgcgccgt gtcggtgtca ccctgcttaa
    35341 ccagagcgtt ctgttacgtg gtgtgaacga taacgcacaa acgctggcaa acctgagtaa
    35401 tgcgttgttc gatgccggcg taatgccgta ttacctgcat gtgctcgata aagtacaggg
    35461 cgcggcgcat tttatggtga gtgatgacga agcacggcag attatgcgtg agttgctgac
    35521 actggtgtcg ggttatctgg tgccgaaact ggcgcgagaa atcggcggcg aacccagcaa
    35581 aacgccgctg gatctccagc tacgccagca gtaactgatt aaacagggaa gttaaacatt
    35641 agcctgatgc gctacgctta tcaggcctac attgtgcatt gaaattgaat ggatttgcaa
    35701 tgaactgtag gccggataag gcgtttacgc cgcatccggc aagagcaaca aacgcaagta
    35761 cagcaacctg tcattaaact gggaaattta atctttgccc gttgcataac ctttaattat
    35821 tcatatacat tgctgttgtt actctttata tagccaacac caataatta
//
LOCUS       DAZKHF020000017        35217 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2201S31241
            SAMN43267800-rid25106413.denovo.017, whole genome shotgun sequence.
ACCESSION   DAZKHF020000017 DAZKHF020000000
VERSION     DAZKHF020000017.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267800
            Sequence Read Archive: SRR30307263
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 35217)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 35217)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 35217)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350581.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350581.2
            Genome Coverage       :: 151x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:21:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,652
            CDSs (total)                      :: 5,557
            Genes (coding)                    :: 5,320
            CDSs (with protein)               :: 5,320
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 237
            CDSs (without protein)            :: 237
            Pseudo Genes (ambiguous residues) :: 0 of 237
            Pseudo Genes (frameshifted)       :: 102 of 237
            Pseudo Genes (incomplete)         :: 130 of 237
            Pseudo Genes (internal stop)      :: 52 of 237
            Pseudo Genes (multiple problems)  :: 41 of 237
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..35217
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267800-rid25106413.denovo.017"
                     /serotype="O 26:H11"
                     /isolate="2201S31241"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-01"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            complement(1..1545)
                     /gene="ycjM"
                     /locus_tag="ACHOSC_RS03615"
                     /old_locus_tag="pgaptmp_000723"
                     /pseudo
     CDS             complement(1..1545)
                     /gene="ycjM"
                     /locus_tag="ACHOSC_RS03615"
                     /old_locus_tag="pgaptmp_000723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415825.4"
                     /note="internal stop; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glucosylglycerate phosphorylase"
     gene            complement(1757..2071)
                     /gene="pspE"
                     /locus_tag="ACHOSC_RS03620"
                     /old_locus_tag="pgaptmp_000724"
     CDS             complement(1757..2071)
                     /gene="pspE"
                     /locus_tag="ACHOSC_RS03620"
                     /old_locus_tag="pgaptmp_000724"
                     /EC_number="2.8.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiosulfate sulfurtransferase PspE"
                     /protein_id="HGU4546716.1"
                     /translation="MFKKGLLALALVFSLPVFAAEHWIDVRVPEQYQQEHVQGAINIP
                     LKEVKERIATAVPDKNDTVKVYCNAGRQSGQAKEILSEMGYTHVENAGGLKDIAMPKV
                     KG"
     gene            complement(2146..2367)
                     /gene="pspD"
                     /locus_tag="ACHOSC_RS03625"
                     /old_locus_tag="pgaptmp_000725"
     CDS             complement(2146..2367)
                     /gene="pspD"
                     /locus_tag="ACHOSC_RS03625"
                     /old_locus_tag="pgaptmp_000725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage shock protein PspD"
                     /protein_id="HGU4546717.1"
                     /translation="MNTRWQQAGQKVKPGFKLAGKLVLLTALRYGPAGVAGWAIKSVA
                     RRPLKMLLAVALEPLLSRAANKLAQRYKR"
     gene            complement(2376..2735)
                     /gene="pspC"
                     /locus_tag="ACHOSC_RS03630"
                     /old_locus_tag="pgaptmp_000726"
     CDS             complement(2376..2735)
                     /gene="pspC"
                     /locus_tag="ACHOSC_RS03630"
                     /old_locus_tag="pgaptmp_000726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="envelope stress response membrane protein PspC"
                     /protein_id="HGU4546718.1"
                     /translation="MAGINLNKKLWRIPQQGMVRGVCAGIANYFDVPAKLVRILVVLS
                     IFFGLALFTLVAYIILSFALDPMPDNMAFGEQLPSSSELLDEVDRELAASETRLREME
                     RYVTSDTFTLRSRFRQL"
     gene            complement(2735..2959)
                     /gene="pspB"
                     /locus_tag="ACHOSC_RS03635"
                     /old_locus_tag="pgaptmp_000727"
     CDS             complement(2735..2959)
                     /gene="pspB"
                     /locus_tag="ACHOSC_RS03635"
                     /old_locus_tag="pgaptmp_000727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009271 - phage shock [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="envelope stress response membrane protein PspB"
                     /protein_id="HGU4546719.1"
                     /translation="MSALFLAIPLTIFVLFVLPIWLWLHYSNRSGRSELSQSEQQRLA
                     QLADEAKRMRERIQALESILDAEHPNWRDR"
     gene            complement(3013..3681)
                     /gene="pspA"
                     /locus_tag="ACHOSC_RS03640"
                     /old_locus_tag="pgaptmp_000728"
     CDS             complement(3013..3681)
                     /gene="pspA"
                     /locus_tag="ACHOSC_RS03640"
                     /old_locus_tag="pgaptmp_000728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage shock protein PspA"
                     /protein_id="HGU4546720.1"
                     /translation="MGIFSRFADIVNANINALLEKAEDPQKLVRLMIQEMEDTLVEVR
                     STSARALAEKKQLTRRIEQASAREVEWQEKAELALLKEREDLARAALIEKQKLTDLIK
                     SLEHEVTLVDDTLARMKKEIGELENKLSETRARQQALMLRHQAANSSRDVRRQLDSGK
                     LDEAMARFESFERRIDQMEAEAESHSFGKQKSLDDQFAELKADDAISEQLAQLKAKMK
                     QDNQ"
     gene            3848..4825
                     /gene="pspF"
                     /locus_tag="ACHOSC_RS03645"
                     /old_locus_tag="pgaptmp_000729"
     CDS             3848..4825
                     /gene="pspF"
                     /locus_tag="ACHOSC_RS03645"
                     /old_locus_tag="pgaptmp_000729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309907.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage shock protein operon transcriptional
                     activator"
                     /protein_id="HGU4546721.1"
                     /translation="MAEYKDNLLGEANSFLEVLEQVSHLAPLDKPVLIIGERGTGKEL
                     IASRLHYLSSRWQGPFISLNCAALNENLLDSELFGHEAGAFTGAQKRHPGRFERADGG
                     TLFLDELATAPMMVQEKLLRVIEYGELERVGGSQPLQVNVRLVCATNADLPAMVNEGT
                     FRADLLDRLAFDVVQLPPLRERESDIMLMAEHFAIQMCREIKLPLFPGFTERARETLL
                     NYRWPGNIRELKNVVERSVYRHGTSDYPLDDIIIDPFKRRPPEDAIAVSETTSLPTLP
                     LDLREFQMQQEKELLQLSLQQGKYNQKRAAELLGLTYHQFRALLKKHQI"
     gene            complement(4945..6210)
                     /gene="puuE"
                     /locus_tag="ACHOSC_RS03650"
                     /old_locus_tag="pgaptmp_000730"
     CDS             complement(4945..6210)
                     /gene="puuE"
                     /locus_tag="ACHOSC_RS03650"
                     /old_locus_tag="pgaptmp_000730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003867 - 4-aminobutyrate transaminase
                     activity [Evidence IEA];
                     GO_process: GO:0009448 - gamma-aminobutyric acid metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-aminobutyrate transaminase"
                     /protein_id="HGU4546722.1"
                     /translation="MSNNEFHQRRLSATPRGVGVMCNFFAQSAENATLKDVEGNEYID
                     FAAGIAVLNTGHRHPDLVAAVEQQLQQFTHTAYQIVPYESYVTLAEKINALAPVSGQA
                     KTAFFTTGAEAVENAVKIARAHTGRPGVIAFSGGFHGRTYMTMALTGKVAPYKIGFGP
                     FPGSVYHVPYPSDLHGISTQDSLDAIERLFKSDIEAKQVAAIIFEPVQGEGGFNVAPK
                     ELVAAIRRLCDEHGIVMIADEVQSGFARTGKLFAMDHYADKPDLMTMAKSLAGGMPLS
                     GVVGNANIMDAPAPGGLGGTYAGNPLAVAAAHAVLNIIDKESLCERANQLGQRLKNTL
                     IDAKESVPAIAAVRGLGSMIAAEFNDPQTGEPSAAIAQKIQQRALAQGLLLLTCGAYG
                     NVIRFLYPLTIPDAQFDAAMKILQDALSD"
     gene            complement(6248..7528)
                     /gene="puuB"
                     /locus_tag="ACHOSC_RS03655"
                     /old_locus_tag="pgaptmp_000731"
     CDS             complement(6248..7528)
                     /gene="puuB"
                     /locus_tag="ACHOSC_RS03655"
                     /old_locus_tag="pgaptmp_000731"
                     /EC_number="1.4.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamylputrescine oxidase"
                     /protein_id="HGU4546723.1"
                     /translation="MTEHTSSYYAASANKYAPFDTLNESISCDVCVVGGGYTGLSSAL
                     HLAEAGFDVVVLEASRIGFGASGRNGGQLVNSYSRDIDVIEKSYGMDTARMLGSMMFE
                     GGEIIRERIKRYQIDCDYRPGGLFVAMNDKQLATLEEQKENWERYGNKQLELLDANAI
                     RREVASDRYTGALLDHSGGHIHPLNLAIGEADAIRLNGGRVYELSAVTQIQHTTPAVV
                     RTAKGQVTAKYVIVAGNAYLGDKVEPELAKRSMPCGTQVITTERLSEDLARSLIPKNY
                     CVEDCNYLLDYYRLTADNRLLYGGGVVYGARDPDDVERLVVPKLLKTFPQLKGVKIDY
                     RWTGNFLLTLSRMPQFGRLDTNIYYMQGYSGHGVTCTHLAGRLIAELLRGDAERFDAF
                     ANLPHYPFPGGRTLRVPFTAMGAAYYSLRDRLGV"
     gene            complement(7530..9017)
                     /gene="puuC"
                     /locus_tag="ACHOSC_RS03660"
                     /old_locus_tag="pgaptmp_000732"
     CDS             complement(7530..9017)
                     /gene="puuC"
                     /locus_tag="ACHOSC_RS03660"
                     /old_locus_tag="pgaptmp_000732"
                     /EC_number="1.2.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707209.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase PuuC"
                     /protein_id="HGU4546724.1"
                     /translation="MNFHHLAYWQDKALSLAIETRLFINGEYTAAAENETFETVDPVT
                     QAPLAKIARGKSVDIDRAVSAARGVFERGDWSLSSPAKRKAVLNKLTDLMEAHAEELA
                     LLETLDTGKPIRHSLRDDIPGAARAIRWYAEAIDKVYGEVATTSSHELAMIVREPVGV
                     IAAIVPWNFPLLLTCWKLGPALAAGNSVVLKPSEKSPLSAIRLAGLAKEAGLPDGVLN
                     VVTGFGHEAGQALSRHNDIDAIAFTGSTRTGKQLLKDAGESNMKRVWLEAGGKSANIV
                     FADCPDLQKAASATAAGIFYNQGQVCIAGTRLLLEESIADEFLALLKQQAQNWQPGHP
                     LDPATTMGTLIDSAHADSVHSFIREGESKGQLLLDGRNAELAAAIGPTIFVDVDPNAS
                     LSREEIFGPVLVVTRFTSEDQALQLANDSQYGLGAAVWTRDLSRAHRMSRRLKAGSVF
                     VNNYNDGDMTVPFGGYKQSGNGRDKSLHALEKFTELKTIWISLEA"
     gene            complement(9292..9849)
                     /gene="puuR"
                     /locus_tag="ACHOSC_RS03665"
                     /old_locus_tag="pgaptmp_000733"
     CDS             complement(9292..9849)
                     /gene="puuR"
                     /locus_tag="ACHOSC_RS03665"
                     /old_locus_tag="pgaptmp_000733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001464354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator PuuR"
                     /protein_id="HGU4546725.1"
                     /translation="MSDEGLAPGKRLSEIRQQQGLSQRRAAELSGLTHSAISTIEQDK
                     VSPAISTLQKLLKVYGLSLSEFFSEPEKPDEPQVVINQDDLIEMGSQGVSMKLVHNGN
                     PNRTLAMIFETYQPGTTTGERIKHQGEEIGTVLEGEIVLTINGQDYHLVAGQSYAINT
                     GIPHSFSNTSAGICRIISAHTPTTF"
     gene            complement(9876..10640)
                     /gene="puuD"
                     /locus_tag="ACHOSC_RS03670"
                     /old_locus_tag="pgaptmp_000734"
     CDS             complement(9876..10640)
                     /gene="puuD"
                     /locus_tag="ACHOSC_RS03670"
                     /old_locus_tag="pgaptmp_000734"
                     /EC_number="3.5.1.94"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415814.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyl-gamma-aminobutyrate hydrolase"
                     /protein_id="HGU4546726.1"
                     /translation="MENIMNNPVIGVVMCRNRLKGHATQTLQEKYLNAIIHAGGLPIA
                     LPHALAEPSLLEQLLPKLDGIYLPGSPSNVQPHLYGENGDEPDADPGRDLLSMALINA
                     ALERRIPIFAICRGLQELVVATGGSLHRKLCEQPELLEHREDPELPVEQQYAPSHEVQ
                     VEEGGLLSALLPECSNFWVNSLHGQGAKVVSPRLRVEARSPDGLVEAVSVINHPFALG
                     VQWHPEWNSSEYALSRILFEGFITACQHHIAEKQRL"
     gene            10852..12270
                     /gene="puuA"
                     /locus_tag="ACHOSC_RS03675"
                     /old_locus_tag="pgaptmp_000735"
     CDS             10852..12270
                     /gene="puuA"
                     /locus_tag="ACHOSC_RS03675"
                     /old_locus_tag="pgaptmp_000735"
                     /EC_number="6.3.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415813.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-putrescine ligase"
                     /protein_id="HGU4546727.1"
                     /translation="METNIVEVENFVQQSEERRGSAFTQEVKRYLERYPNTQYVDVLL
                     TDLNGCFRGKRIPVSSLKKLEKGCYFPASVFAMDILGNVVEEAGLGQEMGEPDRTCVP
                     VLGSLTPSAADPEFIGQMLLTMVDEDGAPFDVEPRNVLNRLWQQLRQRGLFPVVAVEL
                     EFYLLDRQRDAEGYLQPPCAPGTDDRNTQSQVYSVDNLNHFADVLNDIDELAQLQLIP
                     ADGAVAEASPGQFEINLYHTDNVLEACDDALALKRLVRLMAEKHKMHATFMAKPYEEH
                     AGSGMHIHISMQNNRGENVLSDAEGEDSPLLKKMLAGMIDLMPSSMALLAPNVNSYRR
                     FQPGMYVPTQASWGHNNRTVALRIPCGDRHNHRVEYRVAGADANPYLVMAAIFAGILH
                     GLDNELPLQEEVEGNGLEQEGLPFPIRQSDALGEFIENDHLRRYLGERFCHVYHACKN
                     DELLQFERLITETEIEWMLKNA"
     gene            12419..12583
                     /locus_tag="ACHOSC_RS03680"
                     /old_locus_tag="pgaptmp_000736"
     CDS             12419..12583
                     /locus_tag="ACHOSC_RS03680"
                     /old_locus_tag="pgaptmp_000736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4546728.1"
                     /translation="MPMIKSPHGEGGCVCAPPATDWTPPPLLPLLNRFDFRSTRPQTL
                     LRRGGSNYGY"
     gene            12573..13958
                     /gene="puuP"
                     /locus_tag="ACHOSC_RS03685"
                     /old_locus_tag="pgaptmp_000737"
     CDS             12573..13958
                     /gene="puuP"
                     /locus_tag="ACHOSC_RS03685"
                     /old_locus_tag="pgaptmp_000737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707205.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putrescine/proton symporter PuuP"
                     /protein_id="HGU4546729.1"
                     /translation="MAINSPLNIAAQPGKTRLRKSLKLWQVVMMGLAYLTPMTVFDTF
                     GIVSGISDGHVPASYLLALAGVLFTAISYGKLVRQFPEAGSAYTYAQKSINPHVGFMV
                     GWSSLLDYLFLPMINVLLAKIYLSALFPEVPPWVWVVTFVAILTAANLKSVNLVANFN
                     TLFVLVQISIMVVFIFLVVQGLHKGEGVGTVWSLQPFISENAHLIPIITGATIVCFSF
                     LGFDAVTTLSEETPDAARVIPKAIFLTAVYGGVIFIAASFFMQLFFPDISRFKDPDAA
                     LPEIALFVGGKLFQSIFLCTTFVNTLASGLASHASVSRLLYVMGRDNVFPERVFGYVH
                     PKWRTPALNVIMVGIVALSALFFDLVTATALINFGALVAFTFVNLSVFNHFWRRKGMN
                     KSWKDHFHYLLMPLVGALTVGVLWVNLESTSLTLGLVWASLGGAYLWYLIRRYRKVPL
                     YEGDRTPVSET"
     gene            14092..14337
                     /gene="ymjA"
                     /locus_tag="ACHOSC_RS03690"
                     /old_locus_tag="pgaptmp_000738"
     CDS             14092..14337
                     /gene="ymjA"
                     /locus_tag="ACHOSC_RS03690"
                     /old_locus_tag="pgaptmp_000738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YmjA family protein"
                     /protein_id="HGU4546730.1"
                     /translation="MNHDIPLKYFDIADEYATECAEPVAEAERTPLAHYFQLLLTRLM
                     NNEEISEEAQHEMAAEAGINPVRIDEIAEFLNQWGNE"
     gene            14650..16293
                     /gene="sapA"
                     /locus_tag="ACHOSC_RS03695"
                     /old_locus_tag="pgaptmp_000739"
     CDS             14650..16293
                     /gene="sapA"
                     /locus_tag="ACHOSC_RS03695"
                     /old_locus_tag="pgaptmp_000739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein SapA"
                     /protein_id="HGU4546731.1"
                     /translation="MRQVLSSLLVIAGLVSGQAIAAPESPPHADIRDSGFVYCVSGQV
                     NTFNPSKASSGLIVDTLAAQFYDRLLDVDPYTYRLMPELAESWEVLDNGATYRFHLRR
                     DVPFQKTDWFTPTRKMNADDVVFTFQRIFDRNNPWHNVNGSNFPYFDSLQFADNVKSV
                     RKLDNHTVEFRLAQPDASFLWHLATHYASVMSAEYARKLEKEDRQEQLDRQPVGTGPY
                     QLSEYRAGQFIRLQRHDDFWRGKPLMPQVVVDLGSGGTGRLSKLLTGECDVLAWPAAS
                     QLSILRDDPRLRLTLRPGMNVAYLAFNTAKPPLNNPAVRHALALAINNQRLMQSIYYG
                     TAETAASILPRASWAYDNEAKITEYNPAKSREQLKALGLENLTLKLWVPTRSQAWNPS
                     PLKTAELIQADMAQVGVKVVIVPVEGRFQEARLMDMSHDLTLSGWATDSNDPDSFFRP
                     LLSCAAIHSQTNLAHWCDPKFDSVLRKALSSQQLAARIEAYDEAQSILAQELPILPLA
                     SSLRLQAYRYDIKGLVLSPFGNASFAGVYREKQDEVKKP"
     gene            16290..17255
                     /gene="sapB"
                     /locus_tag="ACHOSC_RS03700"
                     /old_locus_tag="pgaptmp_000740"
     CDS             16290..17255
                     /gene="sapB"
                     /locus_tag="ACHOSC_RS03700"
                     /old_locus_tag="pgaptmp_000740"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putrescine export ABC transporter permease SapB"
                     /protein_id="HGU4546732.1"
                     /translation="MIIFTLRRILLLIVTLFLLTFVGFSLSYFTPHAPLQGASLWNAW
                     VFWFNGLIHWDFGVSSINGQPIAEQLKEVFPATMELCILAFGFALIVGIPVGMIAGIT
                     RHKWQDNLINAIALLGFSIPVFWLALLLTLFCSLTLGWLPVSGRFDLLYEVKPITGFA
                     LIDAWLSDSPWRDEMIMSAIRHMILPVITLSVAPTTEVIRLMRISTIEVYDQNYVKAA
                     ATRGLSRFTILRRHVLHNALPPVIPRLGLQFSTMLTLAMITEMVFSWPGLGRWLINAI
                     RQQDYAAISAGVMVCGSLVIIVNVISDILGAMANPLKHKEWYALR"
     gene            17242..18132
                     /gene="sapC"
                     /locus_tag="ACHOSC_RS03705"
                     /old_locus_tag="pgaptmp_000741"
     CDS             17242..18132
                     /gene="sapC"
                     /locus_tag="ACHOSC_RS03705"
                     /old_locus_tag="pgaptmp_000741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter permease SapC"
                     /protein_id="HGU4546733.1"
                     /translation="MPYDSVYSEKRPPGTLRTAWRKFYSDASAMVGLYGCAGLAVLCI
                     FGGWFAPYGIDQQFLGYQLLPPSWSRYGEVSFFLGTDDLGRDVLSRLLSGAAPTVGGA
                     FVVTLAATICGLVLGTFAGATHGLRSAVLNHILDTLLAIPSLLLAIIVVAFAGPSLSH
                     AMFAVWLALLPRMVRSIYSMVHDELEKEYVIAARLDGASTLNILWFAVMPNITAGLVT
                     EITRALSMAILDIAALGFLDLGAQLPSPEWGAMLGDALELIYVAPWTVMLPGAAIMIS
                     VLLVNLLGDGVRRAIIAGVE"
     gene            18132..19124
                     /gene="sapD"
                     /locus_tag="ACHOSC_RS03710"
                     /old_locus_tag="pgaptmp_000742"
     CDS             18132..19124
                     /gene="sapD"
                     /locus_tag="ACHOSC_RS03710"
                     /old_locus_tag="pgaptmp_000742"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter ATP-binding protein
                     SapD"
                     /protein_id="HGU4546734.1"
                     /translation="MPLLDIRNLTIEFKTGDEWVKAVDRVSMTLTEGEIRGLVGESGS
                     GKSLIAKAICGVNKDNWRVTADRMRFDDIDLLRLSARERRKLVGHNVSMIFQEPQSCL
                     DPSERVGRQLMQNIPAWTYKGRWWQRFGWRKRRAIELLHRVGIKDHKDAMRSFPYELT
                     EGECQKVMIAIALANQPRLLIADEPTNSMEPTTQAQIFRLLTRLNQNSNTTILLISHD
                     LQMLSQWADKINVLYCGQTVETAPSKELVTMPHHPYTQALIRAIPDFGSAMPHKSRLN
                     TLPGAIPLLEQLPIGCRLGPRCPYAQRECIVTPRLTGAKNHLYACHFPLNMEKE"
     gene            19126..19932
                     /gene="sapF"
                     /locus_tag="ACHOSC_RS03715"
                     /old_locus_tag="pgaptmp_000743"
     CDS             19126..19932
                     /gene="sapF"
                     /locus_tag="ACHOSC_RS03715"
                     /old_locus_tag="pgaptmp_000743"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter ATP-binding protein
                     SapF"
                     /protein_id="HGU4546735.1"
                     /translation="MIETLLEVRNLSKTFRYRTGWFRRQTVEAVKPLSFTLREGQTLA
                     IIGENGSGKSTLAKMLAGMIEPTSGELLIDDHPLHFGDYSFRSQRIRMIFQDPSTSLN
                     PRQRISQILDFPLRLNTDLEPEQRRKQIIETMRMVGLLPDHVSYYPHMLAPGQKQRLG
                     LARALILRPKVIIADEALASLDMSMRSQLINLMLELQEKQGISYIYVTQHIGMMKHIS
                     DQVLVMHQGEVVERGSTADVLASPLHELTKRLIAGHFGEALTADAWRKDR"
     gene            20000..20353
                     /gene="ycjD"
                     /locus_tag="ACHOSC_RS03720"
                     /old_locus_tag="pgaptmp_000744"
     CDS             20000..20353
                     /gene="ycjD"
                     /locus_tag="ACHOSC_RS03720"
                     /old_locus_tag="pgaptmp_000744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF559 domain-containing protein"
                     /protein_id="HGU4546736.1"
                     /translation="MMDKIKSNARDLRRNLTLQERKLWRYLRSRRFGDFKFRRQHPVG
                     SYILDFACCSARVVVELDGGQHDLAVAYDTRRTSWLESQSWTVLRFWHNEIDCNEEAV
                     LEIILQELNRRSPSP"
     gene            20721..21509
                     /gene="fabI"
                     /locus_tag="ACHOSC_RS03725"
                     /old_locus_tag="pgaptmp_000745"
     CDS             20721..21509
                     /gene="fabI"
                     /locus_tag="ACHOSC_RS03725"
                     /old_locus_tag="pgaptmp_000745"
                     /EC_number="1.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-ACP reductase FabI"
                     /protein_id="HGU4546737.1"
                     /translation="MGFLSGKRILVTGVASKLSIAYGIAQAMHREGAELAFTYQNDKL
                     KGRVEEFAAQLGSDIVLQCDVAEDASIDTMFAELGKVWPKFDGFVHSIGFAPGDQLDG
                     DYVNAVTREGFKIAHDISSYSFVAMAKACRSMLNPGSALLTLSYLGAERAIPNYNVMG
                     LAKASLEANVRYMANAMGPEGVRVNAISAGPIRTLAASGIKDFRKMLAHCEAVTPIRR
                     TVTIEDVGNSAAFLCSDLSAGISGEVVHVDGGFSIAAMNELELK"
     gene            21654..22783
                     /gene="yciW"
                     /locus_tag="ACHOSC_RS03730"
                     /old_locus_tag="pgaptmp_000746"
                     /pseudo
     CDS             21654..22783
                     /gene="yciW"
                     /locus_tag="ACHOSC_RS03730"
                     /old_locus_tag="pgaptmp_000746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309887.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="CMD domain-containing protein"
     gene            22851..24785
                     /gene="rnb"
                     /locus_tag="ACHOSC_RS03735"
                     /old_locus_tag="pgaptmp_000747"
     CDS             22851..24785
                     /gene="rnb"
                     /locus_tag="ACHOSC_RS03735"
                     /old_locus_tag="pgaptmp_000747"
                     /EC_number="3.1.13.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0008859 - exoribonuclease II activity
                     [Evidence IEA];
                     GO_process: GO:0006401 - RNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exoribonuclease II"
                     /protein_id="HGU4546738.1"
                     /translation="MFQDNPLLAQLKQQLHSQTPRAEGVVKATEKGFGFLEVDAQKSY
                     FIPPPQMKKVMHGDRIIAVIHSEKERESAEPEELVEPFLTRFVGKVQGKNDRLAIVPD
                     HPLLKDAIPCRAARGLNHEFKEGDWAVAEMRRHPLKGDRSFYAELTQYITFGDDHFVP
                     WWVTLARHNLEKEAPDGVATAMLDEGLVREDLTALDFVTIDSASTEDMDDALFAKALP
                     DDKLQLIVAIADPTAWIAEGSKLDKAAKIRAFTNYLPGFNIPMLPRELSDDLCSLRAN
                     EVRPVLACRMTLSADGTIEDNIEFFAATIESKAKLVYDQVSDWLENTGDWQPESEAIA
                     EQVRLLAQICQRRGEWRHNHALVFKDRPDYRFILGEKGEVLDIVAEPRRIANRIVEEA
                     MIAANICAARVLRDKLGFGIYNVHMGFDPANADALAALLKTHGLHVDAEEVLTLDGFC
                     KLRRELDAQPTGFLDSRIRRFQSFAEISTEPGPHFGLGLEAYATWTSPIRKYGDMINH
                     RLLKAVIKGETATRPQDEITVQMAERRRLNRMAERDVGDWLYARFLKDKAGTDTRFAA
                     EIVDISRGGMRVRLVDNGAIAFIPAPFLHAMRDELVCSQENGTVQIKGETVYKVTDVI
                     DVTIAEVRMETRSIIARPVA"
     gene            complement(24863..24966)
                     /locus_tag="ACHOSC_RS03740"
                     /old_locus_tag="pgaptmp_000748"
                     /pseudo
     CDS             complement(24863..24966)
                     /locus_tag="ACHOSC_RS03740"
                     /old_locus_tag="pgaptmp_000748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791239.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            25020..27005
                     /gene="pdeR"
                     /locus_tag="ACHOSC_RS03745"
                     /old_locus_tag="pgaptmp_000749"
     CDS             25020..27005
                     /gene="pdeR"
                     /locus_tag="ACHOSC_RS03745"
                     /old_locus_tag="pgaptmp_000749"
                     /EC_number="3.1.4.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic di-GMP phosphodiesterase"
                     /protein_id="HGU4546739.1"
                     /translation="MKTVRESTTLYNFLGSHNPYWRLTESSDVLRFSTTETTEPDRTL
                     QLSAEQAARIREMTVITSSLMMSLTVDESDLSVHLVGRKINKREWAGNASAWHDTPAV
                     ARDLSHGLSFAEQVVSEAHSAIVILDSRGNIQRFNRLCEDYTGLKEHDVIGQSVFKLF
                     MSRREAAASRRNNRVFFRSGNAYEVELWIPTCKGQRLFLFRNKFVHSGSGKNEIFLIC
                     SGTDITEERRAQERLRILANTDSITGLPNRNAMQDLIDHAINHADNNKVGVVYLDLDN
                     FKKVNDAYGHLFGDQLLRDVSLAILSCLEHDQVLARPGGDEFLVLASNTSQSALEAMA
                     SRILTRLRLPFRIGLIEVYTSCSVGIALSPEHGSDSTAIIRHADTAMYTAKEGGRGQF
                     CVFTPEMNQRVFEYLWLDTNLRKALENDQLVIHYQPKITWRGEVRSLEALVRWQSPER
                     GLIPPLDFISYAEESGLIVPLGRWVILDVVRQVAKWREKGINLRVAVNISARQLADQT
                     IFTALKQVLQELNFEYCPIDVELTESCLIENDELALSVIQQFSQLGAQVHLDDFGTGY
                     SSLSQLARFPIDAIKLDQVFVRDIHKQPVSQSLVRAIVAVAQALNLQVIAEGVESAKE
                     DAFLTKNGINERQGFLFAKPMPAVAFERWYKRYLKRA"
     gene            complement(26987..27115)
                     /gene="ymiD"
                     /locus_tag="ACHOSC_RS03750"
                     /old_locus_tag="pgaptmp_000750"
     CDS             complement(26987..27115)
                     /gene="ymiD"
                     /locus_tag="ACHOSC_RS03750"
                     /old_locus_tag="pgaptmp_000750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YmiD"
                     /protein_id="HGU4546740.1"
                     /translation="MQKLPLKEKCLTATANYHPGIRYIMTGYSAKYIYSSTYARFR"
     gene            27153..27326
                     /gene="yciZ"
                     /locus_tag="ACHOSC_RS03755"
                     /old_locus_tag="pgaptmp_000751"
     CDS             27153..27326
                     /gene="yciZ"
                     /locus_tag="ACHOSC_RS03755"
                     /old_locus_tag="pgaptmp_000751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YciZ"
                     /protein_id="HGU4546741.1"
                     /translation="MSEFDAQRVAERIDIVLDILVAGDYHSAIHNLEILKAELLRQVA
                     ESTPDIPKAPWEI"
     gene            27416..28165
                     /gene="yciT"
                     /locus_tag="ACHOSC_RS03760"
                     /old_locus_tag="pgaptmp_000752"
     CDS             27416..28165
                     /gene="yciT"
                     /locus_tag="ACHOSC_RS03760"
                     /old_locus_tag="pgaptmp_000752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator YciT"
                     /protein_id="HGU4546742.1"
                     /translation="MNSRQQTILQMVIDQGQVSVTDLAKATGVSEVTIRQDLNTLEKL
                     SYLRRAHGFAVSLDSDDVETRMMSNYTLKRELAEFAASLVQPGETIFIENGSSNALLA
                     RTLGEQKKNVTIITVSSYIAHLLKDAPCEVILLGGVYQKKSESMVGPLTRQCIQQVHF
                     SKAFIGIDGWQPETGFTGRDMMRTDVVNAVLEKECEAIVLTDSSKFGAVHSYSIGPVE
                     RFNRVITDSKIRASDLMHLEHSKLTVHVVDI"
     gene            28434..28652
                     /gene="osmB"
                     /locus_tag="ACHOSC_RS03765"
                     /old_locus_tag="pgaptmp_000753"
     CDS             28434..28652
                     /gene="osmB"
                     /locus_tag="ACHOSC_RS03765"
                     /old_locus_tag="pgaptmp_000753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="osmotically-inducible lipoprotein OsmB"
                     /protein_id="HGU4546743.1"
                     /translation="MFVTSKKMTAAVLAITLAMSLSACSNWSKRDRNTAIGAGAGALG
                     GAVLTDGSTLGTLGGAAVGGVIGHQVGK"
     gene            complement(28778..29104)
                     /gene="yciH"
                     /locus_tag="ACHOSC_RS03770"
                     /old_locus_tag="pgaptmp_000754"
     CDS             complement(28778..29104)
                     /gene="yciH"
                     /locus_tag="ACHOSC_RS03770"
                     /old_locus_tag="pgaptmp_000754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415798.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stress response translation initiation inhibitor
                     YciH"
                     /protein_id="HGU4546744.1"
                     /translation="MSDSNSRLVYSTETGRIDEPKAAPVRPKGDGVVRIQRQTSGRKG
                     KGVCLITGVDLDDAELTKLAAELKKKCGCGGAVKDGVIEIQGDKRDLLKSLLEAKGMK
                     VKLAGG"
     gene            complement(29104..29841)
                     /gene="pyrF"
                     /locus_tag="ACHOSC_RS03775"
                     /old_locus_tag="pgaptmp_000755"
     CDS             complement(29104..29841)
                     /gene="pyrF"
                     /locus_tag="ACHOSC_RS03775"
                     /old_locus_tag="pgaptmp_000755"
                     /EC_number="4.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004590 - orotidine-5'-phosphate
                     decarboxylase activity [Evidence IEA];
                     GO_process: GO:0006207 - 'de novo' pyrimidine nucleobase
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0044205 - 'de novo' UMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="orotidine-5'-phosphate decarboxylase"
                     /protein_id="HGU4546745.1"
                     /translation="MTLTASSSSRAVTNSPVVVALDYHNRDDALSFVDKIDPRDCRLK
                     VGKEMFTLFGPQFVRELQQRGFDIFLDLKFHDIPNTAAHAVAAAADLGVWMVNVHASG
                     GARMMTAAREALVPFGKDAPLLIAVTVLTSMEASDLVDLGMTLSPADYAERLAALTQK
                     CGLDGVVCSAQEAVRFKQVFGQEFKLVTPGIRPQGSEAGDQRRIMTPEQALSAGVDYM
                     VIGRPVTQSVDPAQTLKAINASLQRSA"
     gene            complement(30033..31202)
                     /gene="lapB"
                     /locus_tag="ACHOSC_RS03780"
                     /old_locus_tag="pgaptmp_000756"
     CDS             complement(30033..31202)
                     /gene="lapB"
                     /locus_tag="ACHOSC_RS03780"
                     /old_locus_tag="pgaptmp_000756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0008653 - lipopolysaccharide metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide assembly protein LapB"
                     /protein_id="HGU4546746.1"
                     /translation="MLELLFLLLPVAAAYGWYMGRRSAQQNKQDEANRLSRDYVAGVN
                     FLLSNQQDKAVDLFLDMLKEDTGTVEAHLTLGNLFRSRGEVDRAIRIHQTLMESASLT
                     YEQRLLAIQQLGRDYMAAGLYDRAEDMFNQLTDETDFRIGALQQLLQIYQATSEWQKA
                     IDVAERLVKLGKDKQRVEIAHFYCELALQHMASDDLDRAMTLLKKGAAADKNSARVSI
                     MMGRVFMAKGEYAKAVESLQRVISQDRELVSETLEMLQTCYQQLGKTAEWAEFLQRAV
                     EENTGADAELMLADIIEARDGSEAAQVYITRQLQRHPTMRVFHKLMDYHLNEAEEGRA
                     KESLMVLRDMVGEKVRSKPRYRCQKCGFTAYTLYWHCPSCRAWSTIKPIRGLDGL"
     gene            complement(31209..31517)
                     /gene="lapA"
                     /locus_tag="ACHOSC_RS03785"
                     /old_locus_tag="pgaptmp_000757"
     CDS             complement(31209..31517)
                     /gene="lapA"
                     /locus_tag="ACHOSC_RS03785"
                     /old_locus_tag="pgaptmp_000757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide assembly protein LapA"
                     /protein_id="HGU4546747.1"
                     /translation="MKYLLIFLLVLAIFVISVTLGAQNDQQVTFNYLLAQGEYRISTL
                     LAVLFAAGFAIGWLICGLFWLRVRVSLARAERKIKRLENQLSPATDVAVVPHSSAAKE
                     "
     gene            complement(31666..32430)
                     /gene="pgpB"
                     /locus_tag="ACHOSC_RS03790"
                     /old_locus_tag="pgaptmp_000758"
     CDS             complement(31666..32430)
                     /gene="pgpB"
                     /locus_tag="ACHOSC_RS03790"
                     /old_locus_tag="pgaptmp_000758"
                     /EC_number="3.1.3.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004609 - phosphatidylserine decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylglycerophosphatase B"
                     /protein_id="HGU4546748.1"
                     /translation="MRSIARRTAVGAALLLVMPVAVWISGWRWQPGEQSWLLKAAFWV
                     TETVTQPWGVITHLILFGWFLWCLRFRIKAAFVLFAILAAAILVGQGVKSWIKDKVQE
                     PRPFVIWLEKTHHIPVDEFYTLKRAERGNLVKEQLAEEKNIPQYLRSHWQKETGFAFP
                     SGHTMFAASWALLAVGLLWPRRRTLTIAILLVWATGVMGSRLLLGMHWPRDLVVATLI
                     SWALVAVATWLAQRICGPLTPPAEENREIAQREQES"
     gene            32600..33190
                     /gene="ribA"
                     /locus_tag="ACHOSC_RS03795"
                     /old_locus_tag="pgaptmp_000759"
     CDS             32600..33190
                     /gene="ribA"
                     /locus_tag="ACHOSC_RS03795"
                     /old_locus_tag="pgaptmp_000759"
                     /EC_number="3.5.4.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003935 - GTP cyclohydrolase II activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP cyclohydrolase II"
                     /protein_id="HGU4546749.1"
                     /translation="MQLKRVAEAKLPTPWGDFLMVGFEELATGHDHVALVYGDISGHT
                     PVLARVHSECLTGDALFSLRCDCGFQLEAALTQIAEEGRGILLYHRQEGRNIGLLNKI
                     RAYALQDQGYDTVEANHQLGFAADERDFTLCADMFKLLGVNEVRLLTNNPKKVEILTE
                     AGINIVERVPLIVGRNPNNEHYLDTKAEKMGHLLNK"
     gene            complement(33254..>35217)
                     /gene="acnA"
                     /locus_tag="ACHOSC_RS03800"
                     /old_locus_tag="pgaptmp_000760"
     CDS             complement(33254..>35217)
                     /gene="acnA"
                     /locus_tag="ACHOSC_RS03800"
                     /old_locus_tag="pgaptmp_000760"
                     /EC_number="4.2.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003994 - aconitate hydratase activity
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="aconitate hydratase AcnA"
                     /protein_id="HGU4546750.1"
                     /translation="GQPVSMLIPDVVGFKLTGKLREGITATDLVLTVTQMLRKHGVVG
                     KFVEFYGDGLDSLPLADRATIANMSPEYGATCGFFPIDAVTLDYMRLSGRSEDQVELV
                     EKYAKAQGMWRNPGDEPIFTSTLELDMNDVEASLAGPKRPQDRVALPDVPKAFAASNE
                     LEVNATHKDRQPVDYVMNGHQYQLPDGAVVIAAITSCTNTSNPSVLMAAGLLAKKAVT
                     LGLKRQPWVKASLAPGSKVVSDYLAKAKLTPYLDELGFNLVGYGCTTCIGNSGPLPDP
                     IETAIKKGDLTVGAVLSGNRNFEGRIHPLVKTNWLASPPLVVAYALAGNMNINLAAEP
                     IGHDRKGDPVYLKDIWPSAQEIARAVEQVSTEMFRKEYAEVFEGTAEWKEINVTRSDT
                     YGWQEDSTYIRLSPFFDEMQATPAPVEDIHGARILAMLGDSVTTDHISPAGSIKPDSP
                     AGRYLQGRGVERKDFNSYGSRRGNHEVMMRGTFANIRIRNEMVPGVEGGMTRHLPDSD
                     VVSIYDAAMRYKKEQTPLAVIAGKEYGSGSSRDWAAKGPRLLGIRVVIAESFERIHRS
                     NLIGMGILPLEFPQGVTRKTLGLTGEEKIDIGDLQNLQPGATVPVTLTRADGSQEVVP
                     CRCRIDTATELTYYQNDGILHYVIRNMLK"
BASE COUNT         8300 a         9186 c         8880 g         8851 t
ORIGIN      
        1 agtcagacaa ttaccatcag cgttacttct ttgaatacgc attacggatg aattaatcgt
       61 atcgatggta aaattattat ccggatgaaa ctcgttatgg ctgcgacgaa gtgtaattaa
      121 acgcgacaac tcatgatata ccgcatgcct taatgtagct tcatcgttca gttctcgggt
      181 tatctcttta ctgtgatatt ttttacggtt aatcgcacgg ttatatccga gtttttcgac
      241 acctgcgtaa tcattacgcg agccaagaat actttgaata tatatcgctg gcacaccggg
      301 gaaacttaac aaaatcgcat gggcgagaat aaacctggcg caacgttctt catcgctact
      361 ctcacggcgg cttaacgcat ccatataggt cacatttatt tcatacggac tgcgtgtacc
      421 gtcgggatta tttttccagt ttactaatgc accttcctgc tgtaacgcct cgaccagctc
      481 taatatttcg ctttcaggca acaagccccg tagcgggttt agcccgatgc catcgtgaga
      541 ggcgaggaag ttaaaccagg tggtgttgct ggaaggtagt gtcaggtttt gcgcccacgc
      601 acaaagcgcc tcaacgttct gtttttgcac cgcatgcagc accagtggcg gcagcgagaa
      661 ctggtacacc atatgtgctt cgtcatcgcc tgcgccaaag taagcaatgt tgtctttatg
      721 cggaacattg gtctcggtaa tgatcactgt acctggcgca acgttatcaa taatcgaccg
      781 taacagtttg ataatcagat gtgttttttc cagatggatg cagcttgttc ccggctcttt
      841 ccacataaag ccaacggcat ccaggcggac atactcagca cctttcgcaa ggtaacagag
      901 taaaacatcc accatcgcca gcaacacttc agggctacgg taattcaggt caatttgatc
      961 gtcactaaag gtggtccata aatggcgcgt tgaatgatcg cgcatctgga atggcgttaa
     1021 taacggtaac gcacgcgggc gagtgacggc gctgagatcg gtttgcgggt caacggcaat
     1081 aaaaaaatct tcaaaacctg gatgctgttg taaatagttt ttaaatcatt cactttttgc
     1141 cgacatatgg ttgcagacaa aatcaaacat taaatgactg cattcaccga gttgctgaat
     1201 atcctgccac tcccccgctt cactggcgac ctgatgataa tcaattaccg aaaagccatc
     1261 atcagatgac catggataaa acggcaacaa atgaacatgt gaaaaaatgc tttgcagcca
     1321 ttgatggtaa aactgattaa atgtgggtaa tggttttaaa tcattgctgt gaaattgatc
     1381 ggcataggta attaacacga catcactttc atcccagtgt tttttacgtc gctgtgtaat
     1441 taatcgtttc gcactctcaa gacgcgttac aagtttctgt aaatgagttg cggtaaatgt
     1501 tccaccgtag atttcgtcca ggtaatccgt aattttctgt ttcatgttgc ctttccgtgg
     1561 tagcggtccc atgaaacgag agactactcc gcagaaaata aactgtcaat tgagcacaag
     1621 ggttttagtc acgcaggaaa tcatttttta ccagggaaaa agcgtatttg tgagctgacg
     1681 caaataatct atggggatgt aaataaggtg tgacagaaaa aaaacggcgc ataagcgccg
     1741 ctcatggtga attcttttaa cctttgacct tcggcattgc gatgtctttc aggccaccgg
     1801 cgttctcaac gtgggtatat cccatctcgc taaggatctc ttttgcttgc cctgactggc
     1861 gtccggcatt gcaatacact ttcacggtgt cgtttttatc cggaacggcg gtggcaatgc
     1921 gctctttcac ttccttcagg ggaatattga tggccccctg aacgtgctct tgctgatact
     1981 gctctggaac acgaacatcg atccagtgtt cagcggcgaa aacgggcagt gaaaacacca
     2041 gtgccagagc aagtaagcct tttttaaaca tgtaagcgtc cttctatgga tgaaaattgc
     2101 catccattat acgcctgaga tttaacaatt tttgcgaaac tcccctcacc ttttataacg
     2161 ctgtgccagt ttattagcag cccgacttaa cagcggttcc agcgccacag ccagcaacat
     2221 tttcagcggt cggcgagcaa ctgattttat cgcccagccc gctacacccg ccgggccata
     2281 gcgcagtgcg gtaagaagta ccagcttgcc tgctaatttg aaaccaggct ttaccttttg
     2341 cccggcctgt tgccagcgag tattcataac tttcctcaca gttgacggaa acggctacgt
     2401 aacgtgaaag tatcggaagt gacataacgt tccatctcgc gtaaacgcgt ttcacttgcc
     2461 gccagttcac ggtcgacttc atccagcaat tcgctgctgg aaggtagctg ctcaccaaag
     2521 gccatgttgt ccggcattgg atcaagcgca aatgacaaaa tgatgtaagc aaccagggta
     2581 aacagcgcca gaccgaagaa aatcgacagc accaccagga tacgcaccag ttttgccggt
     2641 acatcaaaat agttggcaat cccggcgcag acgccgcgga ccatgccctg ctgtggaata
     2701 cgccataatt ttttattgag attaatgccc gccattagcg atccctccag ttcggatgtt
     2761 ctgcatcaag aatagattcc agcgcctgaa tacgttcgcg catccgtttt gcttcatcag
     2821 ccagttgcgc taatcgctgc tgctcacttt gcgacaattc actgcgacca gaacgattgc
     2881 tgtaatgcag ccataaccag atcggtaaaa caaacagcac aaaaatggtt aacggaatag
     2941 ccagaaatag cgcgctcata agtactcctt agacgatgag cggcggcgcg ttcggacgcc
     3001 gcctggatgt tattattgat tgtcttgctt cattttggct tttaattgtg ccagttgttc
     3061 gctgattgca tcatcggctt tcagttcggc aaactgatcg tccagcgatt tttgtttacc
     3121 gaagctgtgg ctttctgctt ccgcttccat ctggtcaata cgacgttcga aagattcgaa
     3181 acgagccatt gcttcatcca gtttgccact gtccagctga cgacgcacat cgcgcgacga
     3241 gtttgccgcc tgatgacgta acatcaatgc ctgctggcga gcgcgtgttt cgctcaattt
     3301 gttttccagc tcaccgatct ctttcttcat gcgtgccagc gtgtcgtcca ccagcgtcac
     3361 ttcatgttcc aggctcttaa tcagatcggt cagtttctgt ttttcaatta acgctgcacg
     3421 tgccagatcc tctctctctt tcagcagcgc cagttcggct ttttcctgcc attcaacttc
     3481 acgcgccgac gcttgttcaa tacggcgagt cagctgtttc ttttctgcca acgcgcgcgc
     3541 cgaagtagaa cgtacttcaa ccagtgtatc ttccatctcc tggatcatca gacgaaccag
     3601 tttctgtgga tcttccgctt tctctaacag agcgttgatg ttggcattca cgatgtcggc
     3661 aaagcgagaa aaaataccca taatgttgtc ctcttgattt ctgcgttcag gattgtcctg
     3721 ctgaactgtt aatacaattt gcgtgccaat tttttatctt tttgatttat aaagatctga
     3781 ttgaagaacc aacagcaaca tgccaggatg agttagcgaa ttacactaac aagtggcgaa
     3841 tttcatcatg gcagaataca aagataattt acttggtgag gcgaacagct ttctcgaagt
     3901 gctggaacag gtttcgcatc tcgcaccgct ggacaaaccg gtgctcatca tcggcgaacg
     3961 cggcaccggt aaagagctga ttgccagccg cctgcattat ctctcctccc gttggcaagg
     4021 gccgtttatt tcccttaact gcgcggcgtt aaatgaaaat ctgctggatt ccgaactgtt
     4081 tggtcacgaa gcgggggcgt ttaccggtgc gcaaaaacgt catcctggga gatttgaacg
     4141 tgccgacggc ggtacgctat ttcttgatga actcgctacg gcaccgatga tggtgcagga
     4201 gaaattattg cgcgtgattg agtacggtga actggagcgc gttggcggca gccagccatt
     4261 gcaggtgaat gtgcggttgg tatgcgcgac gaatgccgat ctcccggcga tggtcaatga
     4321 aggcactttt cgcgctgacc tgctcgaccg actggctttt gatgttgtac aactgccacc
     4381 actgcgcgag cgcgaaagcg acataatgtt gatggcagaa cactttgcca tccagatgtg
     4441 tcgggaaatt aagctgcctc tgttcccggg gtttacagag cgcgccagag aaacattgct
     4501 gaattatcgt tggccgggaa atattcgtga attgaaaaac gtggtggaac gttcagtgta
     4561 tcgccacggc accagcgatt atccgcttga tgacatcatt attgatccct ttaaacggcg
     4621 tccgcctgaa gacgctatcg ccgtttcaga aaccacctcg cttccaacac tgccgctgga
     4681 tttacgtgag tttcagatgc agcaggaaaa agagttgctg caactcagtt tgcaacaagg
     4741 gaaatataac cagaagcgtg cggctgaatt actggggtta acttatcatc agttccgcgc
     4801 gttgttgaaa aagcaccaga tttagcgaag ttgagcaata caacttgccg gatgcggcgt
     4861 gaatgcttta ttcggcatac ataatcaata cgtcgtttgc aggcctgata agcgcagcgc
     4921 atcagacatt attgcgttgg gctattaatc gctcagcgca tcctgcaaaa ttttcattgc
     4981 cgcatcgaat tgcgcatccg gtatggtcag cggatacagg aagcgaatca cgttgccgta
     5041 tgcgccacag gtcagcagga gcagcccctg cgccagcgcg cgttgctgga ttttctgtgc
     5101 aatcgccgct gacggctcgc ccgtttgcgg atcgttaaac tctgccgcaa tcatcgaccc
     5161 caggccgcgt accgcagcaa tggccggaac gctttctttg gcatcaatca acgtgttttt
     5221 gagacgctgg cccagttgat tcgcgcgttc gcagagtgat tctttgtcga taatattgag
     5281 caccgcgtgc gcggcagcca ccgccagcgg gttcccggcg taggtgccac ccaacccgcc
     5341 cggcgcgggc gcgtccataa tattcgcgtt accgaccacg cccgaaagcg gcatcccgcc
     5401 cgcgaggctt ttcgccatcg tcattaagtc cggcttatcg gcgtaatgat ccatggcaaa
     5461 cagtttaccg gtacgcgcaa agccgctttg cacttcatcg gcaatcatca caataccgtg
     5521 ctcgtcgcac aggcggcgaa tggcggcaac cagctctttt ggcgcaacgt tgaaaccgcc
     5581 ctcgccctgc accggttcga aaataatggc cgcgacctgc ttcgcttcga tgtctgattt
     5641 aaacaagcgt tcgatggcgt cgagggagtc ctgtgttgaa atgccgtgta aatctgacgg
     5701 ataaggtacg tgatacaccg aaccagggaa cgggccgaag ccgattttgt acggcgcaac
     5761 ttttccggtc agcgccatgg tcatatatgt acgaccgtgg aagccaccgc taaacgcaat
     5821 cacgccaggt cgtccggtat gggcgcgagc aattttcacc gcgttttcca ccgcttccgc
     5881 accggtagtg aagaatgcgg ttttagcctg cccgctcact ggggcaaggg cgttaatttt
     5941 ctccgccagc gtgacgtagc tttcgtacgg cacaatctga tacgccgtgt gggtaaactg
     6001 ttgcaattgc tgctccaccg ccgcgaccag atcagggtgg cgatgcccgg tattcagcac
     6061 cgcaatgcct gcggcgaaat cgatgtattc gttgccctct acgtccttca gcgtagcgtt
     6121 ttctgccgac tgggcgaaga agttacacat cacgccaacc ccgcgtgggg tagcagaaag
     6181 acgacgctga tggaattcat tgttgctcat gtttcacacc ttttgactct tcactgttaa
     6241 tcggaaatta aacgcccaga cgatcgcgca ggctgtaata agccgcgccc atcgcggtaa
     6301 atggcacgcg cagcgtgcgc ccgccgggga acgggtaatg cggcagattg gcgaaggcat
     6361 cgaaacgttc ggcgtcgccg cgcagcagtt cggcaatcag acgcccggca agatgggtac
     6421 aggtgacgcc gtggccgctg tagccctgca tgtaatagat gttggtatca aggcgaccga
     6481 actgcggcat gcgcgacaag gtcagcagga agttgcccgt ccagcggtaa tcaattttca
     6541 cgcccttcag ctgcgggaag gttttcagca gtttcggcac cacaaggcgc tcaacgtcat
     6601 ccgggtcgcg cgcgccgtag accacgccgc cgccgtacag caggcggttg tcggcggtaa
     6661 gacggtagta atcaagcaga tagttacagt cttccacaca gtagtttttc gggatcagcg
     6721 aacgggctaa atcttccgac agccgttcgg tggtgatcac ctgcgtgccg cacggcatgc
     6781 tgcgtttcgc cagttccggc tctactttat cgcccagata cgcattcccg gcgacaatca
     6841 catacttcgc cgtcacctga cctttggcag ttcgcacaac ggctggcgtg gtgtgctgga
     6901 tctgcgtcac ggcagaaagt tcatacacgc gcccgccgtt gaggcggatg gcgtccgctt
     6961 cgccaatggc gaggtttagc ggatgaatat gcccaccgct gtgatccagc agcgcaccgg
     7021 tatagcgatc gctggctact tcacggcgaa tggcgttggc gtccagcaat tccagctgtt
     7081 tattgccgta gcgttcccag ttctctttct gctcttcaag tgtggcgagc tgtttatcat
     7141 tcatcgccac aaacaggccg ccggggcggt agtcgcagtc aatctgataa cgtttgatac
     7201 gttcgcggat gatctcacca ccttcgaaca tcatgctgcc gagcatacgg gcggtgtcca
     7261 tgccgtagct tttttcgatc acgtcgatgt cgcggctgta ggagttcaca agctgcccgc
     7321 cattgcgccc gcttgcgcca aagccgatgc gtgaggcttc gagaaccact acgtcaaagc
     7381 ccgcttccgc cagatgcagc gcggaagaga gcccggtata gccgccgccg accacgcaaa
     7441 cgtcgcagga aatcgactca ttcagcgtgt cgaatggcgc gtatttattc gcactggcgg
     7501 cgtagtaact gctggtatgt tcggtcattt caggcctcca ggcttatcca gatggttttc
     7561 agttcggtga atttttcaag ggcatgcagg gatttgtcgc gaccgttgcc gctctgctta
     7621 tagccgccaa acggcacggt catatcgccg tcgttgtagt tattgacgaa gacggaacca
     7681 gccttcaggc ggcggctcat gcggtgcgcg cgggagaggt cgcgcgtcca taccgccgcg
     7741 ccaaggccgt actggctgtc gttggcaagc tgtagcgcct ggtcttctga tgtgaaacgc
     7801 gtgaccacca gcaccggacc gaaaatctct tcgcggctta aggacgcatt cgggtctaca
     7861 tccacaaaga tggtcgggcc gatggcggca gccagctcag cgttacgacc atccagcagc
     7921 agttgccctt tgctttcgcc ttcccgaata aagctatgga ccgagtcagc gtgagcgctg
     7981 tcgattaagg tgcccatggt ggttgcggga tcaagtggat ggcccggctg ccagttttgc
     8041 gcctgctgtt ttaacagggc taagaattca tcggcgatgc tctcttccag taacaaacgc
     8101 gttccggcga tgcacacctg gccctggttg tagaagatgc ctgcggcggt ggcgcttgcc
     8161 gctttttgca gatccggaca atcggcgaaa acgatgttgg cgcttttgcc gcccgcttcc
     8221 agccagacgc gtttcatgtt gctctcgccc gcatctttca gcagctgttt cccggtacgg
     8281 gtcgaaccgg taaaggcaat ggcgtcgata tcgttatgac gcgacagcgc ctgccccgct
     8341 tcatgaccaa aacctgtcac cacgttcaaa acaccatccg gcaagcctgc ttctttcgcc
     8401 agccccgcga gacgaatcgc actgagcggt gatttttcag acggtttcag gacaacgctg
     8461 ttccctgctg ccagcgccgg gccaagcttc cagcaggtca gcagcagcgg aaagttccac
     8521 ggtacgatgg cggcaatcac accgaccggt tcacgcacga tcatcgccag ctcatggcta
     8581 ctggtggtcg ccacttcgcc atacactttg tcgatcgctt cggcgtacca gcgaatggcg
     8641 cgcgccgcgc cgggaatatc atcacgcaga ctgtgacgaa tcggtttgcc ggtgtcgaga
     8701 gtttccagca gtgccagctc ttcggcgtgg gcttccatta aatcggtgag tttattcagt
     8761 accgctttac gtttagccgg agaagagagt gaccagtcgc cgcgttcaaa tacgccgcgt
     8821 gctgcgctca ccgcacggtc gatatcgacg ctcttgccgc gggcaatttt cgccagcggt
     8881 gcctgggtga ccggatcaac ggtttcaaag gtttcatttt ccgccgcagc agtatattca
     8941 ccgttaataa ataagcgggt ttcaatggcg agacttaacg ctttatcctg ccagtaagcc
     9001 agatgatgaa aattcattat gactcctgtt tcacgtctat cagatatatg cagtttgtga
     9061 acgtcagggc gcagagatcc ccgggccgta tgcccgttta tttctggcga cattctttat
     9121 atacacttca tccttcaagc cgcctctgcc ttggctgcgt tcgctccccc gaatcactta
     9181 cctgagtaaa ctcatcggga ttcgctcact tgccgccttg atgcaacttg aatgattttg
     9241 tgtatagatt gccgggcgca ttgcgcttac ccggcttaca gaacaaaaag attaaaacgt
     9301 ggtgggcgta tgggcgctga taattcggca aatacctgcc gacgtattac tgaaactgtg
     9361 cgggatgccg gtattaatgg cataactttg ccccgcgacg aggtggtaat cctgaccatt
     9421 aatcgtcaga acaatttcac cttccagtac tgtgcctatt tcctcaccct gatgcttaat
     9481 tctttcccca gtggttgtgc ccggctggta cgtttcaaag atcatcgcca gcgtgcgatt
     9541 cgggttaccg ttatgaacca gcttcattga cacaccctga ctgcccatct caattaagtc
     9601 gtcctgatta atgacgacct gcggctcatc aggtttttcc ggctcggaaa agaattccga
     9661 gagtgacaga ccatacacct tcagcagctt ttgcagcgta ctgatggcag ggctgacttt
     9721 atcttgttct atcgtactga tagcactgtg agtcagcccg gagagttcgg cggcacgacg
     9781 ttgtgaaagc ccctgctgct ggcggatttc cgacaagcgt tttcctggcg ccagtccctc
     9841 atcactcata tttgcatttc cttaaactgt agtggtcaga gtcgctgttt ttcagcgata
     9901 tggtgctgac aagcggtgat aaagccctcg aacaatatac gcgaaagcgc gtactcgcta
     9961 ctgttccatt ccgggtgcca ctgtacgccc agcgcaaaag gatgattgat gacgctaacc
    10021 gcctcaacta agccatccgg cgagcgagct tcaacacgca accgtgggct aacgaccttc
    10081 gccccttgcc catgcagaga gtttacccaa aagttgctac attcaggtaa caacgcagac
    10141 agtaatcccc cctcttcaac ctgaacttca tgcgacggtg catattgctg ttccaccggc
    10201 agttcgggat cttcccgatg ttccagcaat tcaggctgtt cgcacagctt gcgatgcaac
    10261 gaaccaccgg ttgccaccac cagttcctgt aaaccccggc agatggcgaa aatggggatg
    10321 cgcctttcga gtgcggcatt aattaacgcc atgctcagaa gatcacgccc gggatcggcg
    10381 tcaggctcat cgccgttttc accatatagg tgcggctgca cattgctggg actaccagga
    10441 agataaatgc catcgagttt cggcaaaagt tgttcaagta atgacggttc cgccagcgca
    10501 tgtggtagcg caattggcaa gccgcctgcg tggatgatgg cattcaggta cttttcttgc
    10561 agagtctggg tcgcatgacc cttaagcctg ttcctgcaca ttacgacacc gataaccgga
    10621 ttgttcatta tattttccat gctcaatctc acaaagtgga ctaaattatc gccattactg
    10681 cggtttaaat gacctttatg ttcaatattt tttcaatcta gcagtgggtt ttcatttttg
    10741 caaactcaat ttaacatttg acaaacattt agtttgcata cagattcgaa tggtggtcat
    10801 tatattttac gctttgataa cgagcggaaa acaaaccaaa ggcgaagaat catggaaacc
    10861 aatatcgttg aagtagagaa ctttgttcag cagtcagaag agaggcgggg tagcgccttt
    10921 acgcaggaag tgaaacgcta cctggagcgc tacccgaata cgcaatatgt tgatgtttta
    10981 cttactgatt tgaatggttg cttccgtgga aaacgtatcc ctgtttcaag cctgaagaag
    11041 ttggagaaag ggtgttattt cccggcctcg gtgtttgcaa tggatattct gggcaacgta
    11101 gtagaagaag cgggtctggg tcaggaaatg ggcgagccgg atcgtacttg tgttcctgtt
    11161 ctcggttcct taaccccttc tgccgccgat ccagagttta tcggtcagat gctcctgacc
    11221 atggtcgatg aagatggcgc tccctttgac gttgagccgc ggaacgttct taaccgcctg
    11281 tggcagcagc tacgccagcg cggattgttc ccggttgtag cggtagagct ggagttctat
    11341 ttactggatc gtcagcgcga cgctgaaggg tatctgcaac cgccctgcgc gcccggcacc
    11401 gatgaccgca atacacaaag ccaggtttac tccgttgata acctcaacca cttcgccgat
    11461 gtgctcaatg atattgatga actggcgcag ttacagttaa ttcccgccga cggtgcggtc
    11521 gccgaagcct cgccgggtca gtttgaaatc aacctttacc ataccgataa cgtgctggaa
    11581 gcctgcgatg atgcgctggc gctaaaacgc ctggtgcgtc tgatggcaga aaagcataag
    11641 atgcacgcca cttttatggc gaagccgtat gaagagcacg cgggcagcgg aatgcatatc
    11701 catatcagta tgcaaaataa ccgtggcgag aacgtgcttt ctgacgcgga aggcgaagat
    11761 tcgccgctgc tgaaaaagat gctcgccggg atgattgacc tgatgccgtc gtcgatggcg
    11821 ttgctggcac caaacgtgaa ctcgtatcgc cgcttccagc cgggaatgta tgtgccgacg
    11881 caggcgtcgt ggggccataa caaccgcacc gtcgccctgc gtattccgtg cggcgaccgt
    11941 cataatcacc gcgtggaata tcgcgtggcg ggtgccgatg ccaacccata tctggtgatg
    12001 gcagcgattt ttgccggtat tttgcatggc cttgataacg agctgccgtt gcaggaagaa
    12061 gtcgaaggca acgggctgga acaggaaggc ttacccttcc cgattcgcca gagcgatgcc
    12121 ctgggtgagt ttatcgagaa tgatcacctg cgccgctatt taggcgaacg gttttgccat
    12181 gtgtatcacg cctgtaaaaa tgatgagctg ttgcagtttg agcgcctcat cacagaaaca
    12241 gaaattgagt ggatgttgaa aaacgcgtaa ttccccatgc ggggttgttt catcggcctg
    12301 agcggagcca ggcctgacat ttgctggtaa tccagacatt tcccggatgt cgcgttatgg
    12361 agcgcaggcg gcaacgggct tccgttaacg attaagactg tgacgaggaa atgagatgat
    12421 gccgatgatt aaatccccgc atggcgaggg aggctgcgta tgcgcccctc ctgctactga
    12481 ctggactccg ccccctttgc ttcccttgct aaacaggttt gatttcaggt cgacacgacc
    12541 gcaaacctta ttacgcaggg gaggcagcaa ttatggctat taattcacca ctgaatattg
    12601 ctgcgcaacc cggcaaaacc cgtctgcgaa aatcactgaa attgtggcag gtggtaatga
    12661 tgggtctggc ctatctcacg ccgatgaccg tatttgatac ttttggtatt gtctccggca
    12721 ttagcgacgg tcacgttcca gcgtcctatt tgctggcgct ggcgggcgtg ctgtttaccg
    12781 ctatcagcta cggcaaactg gttcgccagt ttccggaggc cggttcggcc tatacctacg
    12841 cgcaaaagtc gattaatccg cacgtcggat ttatggtcgg ctggtcatca ctgctggatt
    12901 atctcttttt gccaatgatc aacgttttgt tggcgaaaat ctatctctcc gccctcttcc
    12961 cggaagtgcc gccgtgggtg tgggtggtaa ccttcgtcgc cattttaacc gccgcgaatc
    13021 tgaagagcgt caacctggtc gctaacttca ataccctgtt tgtactggtg caaatctcca
    13081 tcatggtggt gtttatcttc ctggtggttc agggactgca taaaggagaa ggcgttggca
    13141 ccgtctggtc acttcagccg tttatcagcg agaacgcgca cctgatcccg attattaccg
    13201 gtgcgacgat tgtctgtttc tcgttcctcg gtttcgatgc ggtgaccacg ctttcggaag
    13261 agacaccgga cgccgcacgc gtgatcccga aagccatctt cctgacggcg gtatacggcg
    13321 gcgttatctt tatcgcggcg tcgttcttta tgcagctgtt ctttcccgat atcagccgct
    13381 ttaaagaccc ggacgccgca ctgcctgaaa ttgcgctgtt cgtcggcggc aagctctttc
    13441 agtcgatttt cctctgcacc acgtttgtga acacattagc gtctggcctg gcttcacatg
    13501 ccagcgtgtc gcgtctgctg tatgtgatgg ggcgtgacaa tgtgtttccg gagcgcgtgt
    13561 ttggctatgt gcacccaaaa tggcggactc cggcactcaa cgtcattatg gtcgggattg
    13621 tcgcgctgtc ggcgctgttc tttgatttag tcaccgcgac agcactgatt aacttcggtg
    13681 ctctggtggc gtttaccttc gtgaatctgt cggtgtttaa tcatttctgg cggcgtaaag
    13741 gaatgaataa aagctggaag gatcacttcc actatttgct gatgccgctg gttggcgcgc
    13801 tgacggtggg tgtgctgtgg gttaacctag agtcaacgtc actgacactc ggtctggtat
    13861 gggcttcgtt aggcggcgca tatttgtggt atctgatccg ccgctatcgc aaagtgccgc
    13921 tgtacgaagg tgacagaacg ccggtgagcg aaacgtaata gccggatgcg cggcgcatta
    13981 tcctgcctga gggtaacgcg ccgcgccatt aactataaaa agatgtatga cccggatcac
    14041 gagtgctatc atccgccctt atgcttagcg cattaacccc aggggcaaat aatgaaccac
    14101 gatatcccgc taaaatattt tgatattgcc gatgagtacg cgaccgaatg cgcagagcct
    14161 gtcgcagagg ctgaacgcac accgctggcc cactacttcc agttactgct cacccgtttg
    14221 atgaacaatg aagagatcag cgaagaagcc caacatgaaa tggctgctga agcggggatt
    14281 aatcccgtac gcattgatga gatcgcagag tttttgaatc aatggggtaa tgagtaatta
    14341 tttaattgca gacgatcggt cagtatcgtt gtccggataa agcgttcacg ccgcatccgg
    14401 ctaacaatga cacgcactgc agcgtgcttt aatgggtttc tcacgatcgt accgctctgc
    14461 tcctcgctaa gttgttctgt gagttatagt tatagagaaa tcacgggtac gaatgttgaa
    14521 cagatggcga cgccacctgt tcaacgacca accacacggc cctcattacg acccctgaac
    14581 acaaacggta atggcaaatg tcattaaata ttagtaagat agcctcttta cataaaatcc
    14641 cttaatatta tgcgccaggt attatcgtct cttttggtga ttgctggact tgtgagtggt
    14701 caggcaatcg ccgcgcctga atcccccccg catgctgata tccgcgacag cggttttgtc
    14761 tattgcgtca gcgggcaagt caacaccttt aacccatcca aagcgagcag tgggttaatt
    14821 gtcgataccc ttgccgccca gttttatgat cgactgctgg atgtcgatcc ctatacctat
    14881 cgcctgatgc cggaacttgc cgaaagctgg gaagtactcg acaacggcgc gacctatcgc
    14941 ttccacctgc gtcgcgatgt tccgtttcaa aaaaccgact ggtttactcc cactcgtaaa
    15001 atgaatgccg acgatgtggt gtttaccttc cagcgaattt ttgaccgcaa caacccgtgg
    15061 cataacgtca acggcagcaa cttcccctac ttcgacagcc tgcaatttgc cgataacgtg
    15121 aaaagcgtcc gcaaactgga taatcatacc gttgagttcc gactcgccca gccggatgct
    15181 tcttttttgt ggcacctcgc aacccattat gcttcggtca tgtcggcaga atatgcccgg
    15241 aagttagaga aagaagatcg ccaggagcaa ctcgaccgtc aaccggtcgg caccggtccg
    15301 tatcagttgt cggaataccg cgccgggcaa tttattcgcc tacaacgtca tgatgacttc
    15361 tggcgcggta aaccgttaat gccgcaggta gtggtggatt taggctccgg cggcaccgga
    15421 cgtctgtcga aactcctgac cggggaatgc gacgttctgg cctggcctgc tgccagccag
    15481 ctatccattt tgcgtgacga cccgcgcttg cgtttaacgc tgcgtcctgg gatgaacgtc
    15541 gcctatctgg catttaacac cgccaaaccg ccgctaaata atcccgctgt ccgccatgca
    15601 ctggcactgg cgattaataa ccagcgcctg atgcaatcca tctattatgg tacggctgaa
    15661 acggcggcct ctattttacc gcgcgcctcg tgggcctatg acaacgaggc taaaattact
    15721 gaatacaatc cggcgaaatc gcgcgaacag ttgaaggcgt tggggctgga aaacttaacg
    15781 ctgaaactgt gggtgcccac acgttcgcag gcgtggaacc ccagtccact gaaaactgcc
    15841 gaactgattc aggcggatat ggcgcaggtt ggcgtaaaag tggtgattgt gccggtagaa
    15901 ggtcgctttc aggaggcgcg gttgatggat atgagccatg atctgacgtt atccggttgg
    15961 gcgacggaca gtaacgaccc ggacagtttc ttccgtcctt tactgagctg cgcggcaatt
    16021 cattcacaga ccaacctcgc ccactggtgc gatccgaaat tcgacagcgt gttgcgtaag
    16081 gcgctctcct cgcagcagct ggcggcgcgt attgaagcct atgacgaagc gcagagtatt
    16141 ctggcgcagg aattgcccat tttgccgctg gcgtcgtcat tgcgtttgca ggcctatcgg
    16201 tacgatatca aaggtctggt acttagcccg tttggtaacg cctcctttgc tggggtgtat
    16261 cgcgagaaac aggatgaggt gaaaaaacca tgattatctt caccttacgc cgcattttgt
    16321 tattgattgt caccttgttc ctgctgacct tcgttggctt cagcttgagc tatttcaccc
    16381 ctcatgcgcc gttacaaggc gcgtcactgt ggaatgcctg ggtgttctgg tttaacggcc
    16441 tgatccactg ggattttggt gtttccagca ttaacggtca accgattgcc gaacagttaa
    16501 aagaggtctt cccggcgacg atggaactgt gcatcctcgc cttcggtttt gcgctgattg
    16561 ttggcatccc cgtggggatg attgccggga ttacgcgcca taagtggcag gataacttga
    16621 tcaacgccat cgccttactg ggcttttcaa tcccggtgtt ctggctggcg cttctgttga
    16681 cgctgttttg ttcactcacg ctaggttggt tgcccgtttc agggcgtttc gatctgctct
    16741 acgaagtgaa accgattacc ggttttgcgt tgattgatgc ctggctttcg gactcaccat
    16801 ggcgggatga aatgatcatg agcgcaatcc gccatatgat attgcccgtg atcaccctgt
    16861 cggttgcgcc aacaactgaa gtgatccgcc tgatgcgtat cagcactatc gaagtgtatg
    16921 accagaacta tgtaaaagcg gcggcgaccc gcggtttgtc gcgctttact attttgcgtc
    16981 gccacgttct gcataacgcg ttaccgccgg ttattcctcg cctgggctta cagttttcca
    17041 ccatgctgac gctggcgatg atcaccgaaa tggtttttag ctggccgggt ttagggcgct
    17101 ggttaattaa cgccatccgc cagcaggatt acgcagccat ttccgccgga gtgatggtgt
    17161 gtggctcact ggtcattatt gttaacgtga tttctgatat tttgggtgcc atggctaacc
    17221 ctctgaaaca taaggaatgg tatgccttac gatagcgtat acagcgaaaa gcgcccgccg
    17281 ggcacgctgc gtaccgcctg gcgcaaattt tatagtgacg cctctgcgat ggtcggcctg
    17341 tacggctgcg cggggctggc tgtactgtgt atttttggcg gctggtttgc gccttacggc
    17401 atcgaccagc aatttctcgg ttatcaatta ttgccgccgt catggtcgcg ctatggcgaa
    17461 gtttctttct tcctggggac tgacgacctc ggacgcgatg ttttaagccg tttactgagc
    17521 ggagctgcgc caaccgtggg tggcgcattt gtggtgacgc ttgccgcgac gatctgtggc
    17581 ctggtactgg gcaccttcgc cggggcgaca cacggcctcc gctcagcggt gcttaaccat
    17641 attctggata ccttactggc gatcccttcg ctgctgttgg caattatcgt tgtagcgttt
    17701 gccggaccga gtttgtccca cgctatgttt gctgtctggc tggcattgct gccgcgtatg
    17761 gtacgttcga tttacagcat ggtgcatgac gaactggaaa aagagtacgt tatcgccgcc
    17821 cgtctggatg gcgcatcaac gctgaatatt ctctggtttg ctgtgatgcc aaacatcacc
    17881 gccgggctgg tcaccgagat cacccgcgca ctgtcgatgg caattctcga tatcgccgcg
    17941 ctgggctttc tcgatctcgg cgcacaactc ccctcgcctg aatggggagc gatgctcggt
    18001 gatgcgctgg aactgattta tgtcgccccg tggactgtca tgctgccagg tgcggcaatt
    18061 atgattagcg tgttgttggt taacctcctt ggcgatggcg ttcgtcgtgc gattattgcg
    18121 ggggtggaat aatgccatta ctcgatattc gtaacctgac cattgaattt aaaaccggtg
    18181 atgagtgggt taaagccgtc gaccgggtaa gtatgacctt aaccgaaggt gaaatccgcg
    18241 gtcttgttgg tgaatccggt tccggcaaga gtttgattgc gaaagcgatt tgtggggtga
    18301 ataaagataa ctggcgtgtt actgctgacc gtatgcgttt tgatgatatc gatttgctgc
    18361 gtctctccgc acgcgaacgg cgcaaactgg ttggccataa cgtgtcgatg attttccagg
    18421 aaccgcagtc gtgtcttgac ccttcagaac gtgtgggtcg ccagttgatg caaaacatcc
    18481 cagcctggac ctataaaggc cgttggtggc agcgttttgg ctggcgcaaa cgccgtgcca
    18541 ttgaactgct gcaccgcgtg gggattaaag atcacaaaga tgcgatgcgc agttttccct
    18601 atgagttgac cgaaggtgaa tgtcagaaag tgatgatagc cattgcactg gcgaatcaac
    18661 cgcgtctgct gattgctgac gaaccgacca actcaatgga gccaacaacc caggcgcaaa
    18721 tctttcgcct gctgacgcgt ctcaaccaaa acagcaatac cactattttg cttatcagcc
    18781 atgacttaca aatgcttagc caatgggcgg ataaaattaa cgtgctttac tgcggtcaaa
    18841 cagtggaaac cgcgccgagt aaggagttgg tgacgatgcc acatcatcct tatacccagg
    18901 cgctgatccg cgcgatacca gacttcggca gcgcgatgcc gcataaaagt cgcctcaata
    18961 cgctgcccgg cgctatccca ctgctggaac agttaccgat tggttgtcgt ctggggccac
    19021 gttgcccgta tgcacaacga gaatgcattg tgacgccacg tttgacgggg gcgaaaaatc
    19081 atctctatgc ctgtcatttc ccgctgaaca tggagaaaga gtgagatgat cgaaacgctg
    19141 cttgaagtgc gtaatttaag taaaacgttc cgctaccgga ccggctggtt tcgtcgtcag
    19201 accgtagaag cggtaaaacc cttgagcttt acgctacgtg aaggccagac actggcgatt
    19261 attggcgaga atggttcggg taaatccacg ctggcaaaaa tgctggcggg aatgatagag
    19321 cctaccagcg gcgagttatt gattgacgat catccactgc attttggcga ttattccttc
    19381 cgcagtcagc gtattcgcat gatttttcag gatccttcga cctcgttgaa tccccgtcaa
    19441 cgtatctcgc aaattctgga ttttccactg cgcctgaaca ccgacctgga accggaacag
    19501 cgtcgcaaac agattattga aacgatgcgt atggttgggc tgctaccgga tcacgtcagt
    19561 tactatccgc atatgttggc acccggacaa aaacagcgtc tgggtctggc gcgcgcgctg
    19621 atattgcgcc caaaagtcat tattgccgat gaagccctcg cttcactgga tatgtcgatg
    19681 cgttcgcagt tgattaatct gatgctggag ttacaggaaa aacagggcat ttcgtatatt
    19741 tatgttaccc agcatatcgg aatgatgaag cacattagcg accaggtgct ggtgatgcat
    19801 cagggcgagg ttgtcgaacg aggcagcacc gcggatgtgc tggcatcgcc gctgcatgaa
    19861 ctcaccaaac ggctgattgc cgggcacttt ggcgaagcat tgacagcgga tgcgtggcgt
    19921 aaagatcgct aattttctga atcctccttc cagattatcc acctcattct gacaagagat
    19981 taaatcttgt cagaatgagg tgatggataa aataaaatca aatgcgcgtg atttaagacg
    20041 caatctcact ttgcaggaac gaaagctctg gcgatatctt cgcagccgac gttttggtga
    20101 tttcaaattt cgccgtcaac atccggtggg gagctacatt ctcgattttg cttgctgctc
    20161 ggcgcgcgta gtcgttgagc tggatggtgg gcagcatgat ttggcagttg cctatgatac
    20221 caggcgcact agctggcttg agtcgcagag ctggaccgtg ctgcgtttct ggcataacga
    20281 gattgattgt aatgaggagg cggtgctgga gattattctg caggaactga accgccggtc
    20341 accctcaccc tgaaagggcg agggggccga ccgagccgaa tagctgttgt ggtgaaaaca
    20401 tggagacagt gctggagaat attcggcaag gtctgaacca tcccagccat cgccataaaa
    20461 gggttagggg ctgtatgagc ctgtttgttg ctggggtaac aatatttgca caatatggtc
    20521 ccctcgcccc tctggggaga gggttagggt aaggggaaaa gcgcccaccc tgccgcagcc
    20581 tgctccggtc ggacctggca actatagcta ctcacagcca ggttgattat aataaccgtt
    20641 tatctgttcg tactgtttac taaaacgacg aatcgcctga ttttcaggca caacaagcat
    20701 caacaataag gattaaagct atgggttttc tttccggtaa gcgcattctg gtaaccggtg
    20761 ttgccagcaa actatccatc gcctacggta tcgctcaggc gatgcaccgc gaaggagctg
    20821 aactggcatt cacctaccag aacgacaaac tgaaaggccg cgtagaagaa tttgccgctc
    20881 aattgggttc tgacatcgtt ctgcagtgcg atgttgcaga agatgccagc atcgacacca
    20941 tgttcgctga actggggaaa gtttggccga aatttgacgg tttcgtacac tctattggtt
    21001 ttgcacctgg cgatcagctg gatggtgact atgttaacgc cgttacccgt gaaggcttca
    21061 aaattgccca cgacatcagc tcctacagct tcgttgcaat ggcaaaagct tgccgctcca
    21121 tgctgaatcc gggttctgcc ctgctgaccc tttcctacct tggcgctgag cgcgctatcc
    21181 cgaactacaa cgttatgggt ctggcaaaag cgtctctgga agcgaacgtg cgctatatgg
    21241 cgaacgcgat gggtccggaa ggtgtgcgtg ttaacgccat ctctgctggt ccgatccgta
    21301 ctctggcggc ctccggtatc aaagacttcc gcaaaatgct ggctcattgc gaagccgtta
    21361 ccccgattcg ccgtaccgtt actattgaag atgtgggtaa ctccgcggca ttcctgtgct
    21421 ccgatctctc tgccggtatc tccggtgaag tggtccacgt tgacggcggt ttcagcatcg
    21481 ctgcaatgaa cgaactcgaa ttgaaataat cattctgttg gtaaatattg ggcggcgttc
    21541 tgccgcccgt tatctctgtt atacctttct gatatttgtt atcgccgatc cgtctttctc
    21601 cccttcccgc cttgcgtcag gataacgatt tcctttacga ccaaggagcg cccatggaac
    21661 aacgccacat caccggcaaa agccactggt atcatgaaac gcaatccagt actgcggagt
    21721 atgacgttct gcctctggtc ccggaagccg caaaggtcag cgatcccttt ctgctcgacg
    21781 tgatccttga tgaagaaacg ctggctccct tcctttcatg gctggtccct gcgcgcgttc
    21841 ttgcagtgga attgttccct gaccagctta ccgtgacccg ttcacagacc ttcaccgctt
    21901 atgaacgctt gtctacggcc ctgacggttg ctcaggtttg cggcgtccag cggttatgta
    21961 actactattc ggcgcgactt acgccgctcc ccgggcctga ttcctccagg gaaagcaatc
    22021 ataggttggc acaaatcacg caatatgccc gccaactggc tagctcgcct tctattatcg
    22081 acaaccgatc gcgccagcat ctgaatgacg tcggtcttac tgccggggac tgtgtgatca
    22141 ttaaccaaat cattggtttt attggctttc aggcgcggac cattgcgaca tttcaggctt
    22201 atctcgggca cccggtacgc tggttacccg gtctggaaat acaaaactac gccgacgcgt
    22261 cactgtttgc tgatgaatca atacgctggc gaagcagcta tgaggtggaa aaactacctg
    22321 aagagtacac aaaaagttca acagcagaac tttgccaact ggccgaaaca ctctctctct
    22381 ccaccctatt tcactttccc ttctcgaaag gttgttaaac agtacacggg ttaatacaca
    22441 gccggataat aagcttgcgg cgttgttatg cgcgcggata aatggcagtc ctgcttgttt
    22501 tgccgcctgt atggattcat caaatgaata taaaaaaatc agcccccttc tgcgcaaggg
    22561 cgaaaatgaa attaaccaat gggctgaccg tcattctgtt gagcacgcta ccgttcaggc
    22621 gatacaatgg ctgacccgag cacccgatcg ctttagcgcc gcccagttca gccctttact
    22681 cgaacacgaa aaatcatcaa cgcagattat taatctgctg gtatggagcg ggctgtatgg
    22741 ctggataaat cgcttaaaaa tcgcgttggg tgagacatat taaccttgcc gcgtcagaca
    22801 gattcgcgta aaactgtcag ccgctctaat ggccaccaaa atagacaatt atgtttcagg
    22861 acaacccgct gctagcgcag cttaaacagc aactgcattc ccagacgcca cgcgctgaag
    22921 gggtggtaaa agccacagaa aaaggctttg gcttcctgga agtcgacgcg caaaaaagtt
    22981 atttcattcc gccgccgcag atgaaaaaag tcatgcatgg cgaccgaatt atcgcggtga
    23041 tccacagtga aaaagaacgt gaatccgcag agccagaaga actggttgaa ccgttcctga
    23101 ctcgtttcgt gggtaaagtt cagggcaaaa atgaccgtct ggccatcgtt cctgatcatc
    23161 cactcttaaa agacgccatt ccttgccgcg cagcccgtgg cctgaaccac gagtttaaag
    23221 aaggcgactg ggcggttgcc gaaatgcgcc gtcatccgct gaaaggcgat cgttctttct
    23281 atgctgaact gacacaatac atcacttttg gtgacgacca ctttgtaccg tggtgggtta
    23341 cccttgcgcg ccataatctg gaaaaagaag caccagacgg cgtcgctacc gcaatgctcg
    23401 atgaaggtct ggttcgtgaa gatctgaccg cgctggattt tgtcaccatc gacagtgcca
    23461 gcacagaaga tatggatgac gcccttttcg ctaaggcgtt gccggatgac aaacttcagc
    23521 tgattgtggc gattgccgat ccaaccgcgt ggattgctga aggcagcaag ctggacaaag
    23581 ccgcgaaaat tcgcgcattc accaactatc tgcctggctt caacatccct atgctgcctc
    23641 gcgagctttc tgacgatctc tgctcactgc gcgccaatga agtccgcccg gtactggcat
    23701 gccgcatgac gctctccgct gatggcacca ttgaagataa tatcgaattc tttgccgcca
    23761 ccatcgaatc caaagcaaag ctggtgtatg accaggtttc tgactggctg gaaaataccg
    23821 gtgactggca gcctgaaagt gaagcaattg ccgaacaagt ccgtttgcta gcgcaaattt
    23881 gccaacgccg cggcgagtgg cgtcataacc acgcactggt gtttaaagat cgcccggatt
    23941 accgctttat tctcggtgaa aaaggtgaag tgctggatat cgtcgccgag cctcgtcgca
    24001 ttgccaaccg tatcgtcgaa gaagcgatga ttgccgctaa catttgtgcg gcccgcgtac
    24061 tgcgcgataa gctcggtttt ggcatctata acgtgcatat gggctttgat ccggcgaatg
    24121 ccgacgcgct ggcagcgttg ctgaaaacgc acggtctgca tgtcgatgcc gaagaagtgc
    24181 tcacgctgga cggtttctgc aaactgcgtc gtgaactgga cgcgcaacca actggtttcc
    24241 tcgacagccg cattcgtcgc ttccagtcat ttgctgaaat tagcactgaa cccggtcctc
    24301 actttggcct cggtctggaa gcatacgcca cctggacttc gccgatccgt aaatatggcg
    24361 acatgatcaa ccaccgtctg ctgaaagcgg ttatcaaagg cgaaactgcg acgcgcccac
    24421 aggatgaaat cactgtccaa atggccgagc gtcgccgtct caaccggatg gcagaacgtg
    24481 atgttggtga ctggttatac gcacgcttcc tgaaagacaa agccgggacc gacacccgtt
    24541 tcgcagcgga aattgtcgat atcagccgtg gcggcatgcg tgttcgtttg gttgataacg
    24601 gcgctatcgc ctttattccg gcacctttct tacacgctat gcgcgatgaa ctggtttgca
    24661 gccaggaaaa cggcactgta caaattaaag gtgaaacggt ttacaaagtg actgacgtta
    24721 ttgacgtcac tattgccgaa gtccgcatgg aaacccgcag cattattgcg cgcccggtcg
    24781 cgtaatctcc tttcacggcc cattcctcat ggatgggccg tttatttccc cgctctacct
    24841 tcgttatatc caagcagatt ttttaactga ctttcgtttg aaaactggcg tttttccaaa
    24901 actggattac aaataaatat ataaaaaaac aatgcgttta gccgccgtta atttcatgta
    24961 agtcatgttg ttccttttat ttaaccgcgg actccgcagt taaccggagg atatgcatca
    25021 tgaaaaccgt tagggagtcc acaacgttgt acaactttct cggatcgcac aatccatact
    25081 ggcggttgac ggaaagcagc gatgttttgc gcttttctac caccgaaacc acagaacctg
    25141 atcgtacatt gcagttatct gccgaacagg ctgctcgcat cagggaaatg acggtcatca
    25201 cctccagcct gatgatgagt ctgaccgtcg atgaaagcga tctttctgtg catctggtag
    25261 gacgaaaaat caataaacgg gaatgggctg gcaacgcgtc tgcatggcat gacacaccgg
    25321 cggttgctcg tgatttatca cacgggcttt cctttgctga gcaggtagtt tctgaagcac
    25381 attccgcaat agtgattctc gacagccggg ggaatatcca acgcttcaat cggttatgtg
    25441 aagattacac agggttgaaa gaacacgacg tcattgggca aagcgtgttt aaactgttta
    25501 tgagccgtcg tgaagctgcg gcatccaggc gcaataaccg tgtatttttt cgaagcggca
    25561 atgcatatga agtcgaactg tggataccaa catgtaaagg ccagcggctg tttctgtttc
    25621 gcaataaatt tgtccacagc ggcagtggca aaaacgagat ttttttaatc tgttccggca
    25681 ccgacattac cgaagagcgc cgcgctcagg agcgactgcg tattctggca aataccgaca
    25741 gtatcaccgg actgccgaat cgtaacgcaa tgcaggattt aatcgatcac gctattaatc
    25801 atgcagataa caataaagtt ggggttgtgt atcttgattt ggataatttc aaaaaggtca
    25861 acgacgccta tgggcatttg tttggtgacc agttattacg cgacgtgtca ttggctattt
    25921 taagctgtct cgaacatgac caggtgttgg cgcgtccagg tggggatgag tttctggtac
    25981 tggcatccaa cacctcacaa agcgcgctgg aagcaatggc atcacgaatt ttgacccgct
    26041 tacggctccc ctttcgcatt ggtttaattg aagtttatac cagctgttca gtaggtattg
    26101 cactctctcc cgaacatggt tcagacagca cggctattat tcgtcacgcc gacacagcaa
    26161 tgtacacagc gaaggaaggc ggacgaggac aattttgcgt ttttacccca gaaatgaatc
    26221 aacgggtatt tgaatatctc tggctggata ccaacttgcg taaagcactg gaaaacgatc
    26281 agttggttat tcactatcaa ccgaaaatca cctggcgtgg cgaagtgcgc agtctggaag
    26341 cactagtacg ttggcagtca cctgaacgtg ggttgattcc accgttggac ttcatttcct
    26401 acgccgaaga gtcagggcta attgtgcctt taggccgttg ggtgattctc gatgtcgtac
    26461 gccaggtggc aaagtggcgg gaaaaaggca taaacctgcg agtggcggta aatatttctg
    26521 cacgtcagct cgccgatcaa accattttca ccgccctgaa acaggttctc caggaactca
    26581 attttgaata ctgccctata gatgttgaac tgacagagag ttgtctgatt gagaatgatg
    26641 aactggcact gtctgttatt caacaattta gccaactagg tgcgcaagtg catctggatg
    26701 attttggtac cggctactct tcactttcgc aactggcgcg ctttccgatc gatgccatca
    26761 aacttgacca ggtttttgtt cgagatattc acaaacaacc tgtctcgcag tcactggtcc
    26821 gggcgatcgt cgctgtggcc caggcattga atcttcaggt gatcgccgaa ggtgtagaga
    26881 gtgcaaagga agatgctttt ttaaccaaga acgggatcaa tgagcggcaa ggatttttgt
    26941 ttgccaaacc gatgcccgcc gtcgccttcg aacgctggta taaacgctat ctgaagcgcg
    27001 cataagtaga tgaatatata tacttagcgc tatatcccgt cattatatat cgtattcctg
    27061 gatgataatt agccgttgct gttaaacact tctcttttag cggcaatttt tgcattattc
    27121 attttccccc ttttgctata aggatgtcct tgatgtctga atttgatgcc cagcgcgttg
    27181 ccgaacgtat tgatattgtg ttagatatcc ttgtggcagg cgattaccac tctgcgatcc
    27241 ataatctcga gatacttaaa gcagaactct tgcgccaggt tgctgaatct acgccagaca
    27301 tcccgaaggc accgtgggag atttaacaat cgtgccttca ggttttccta tttgaggatg
    27361 cccggcgcgt cagtattttt ttatttagta ttataacgtt ataagaatta cagcgatgaa
    27421 ttcccgacaa caaactattc tacagatggt cattgaccag ggtcaggtta gcgtaaccga
    27481 tctggcaaaa gccactggag tttctgaagt taccattcgc caggatctca acaccctcga
    27541 aaaactgagt tacctccgcc gtgcacatgg ctttgcagtt tcgcttgata gtgatgacgt
    27601 cgaaacccgt atgatgagca actatacgct gaagcgtgaa ctcgccgagt ttgccgcgtc
    27661 tctggttcaa ccgggcgaaa ccatctttat cgaaaatggt agcagtaatg ccctgctcgc
    27721 ccggactctg ggcgaacaga agaaaaatgt cactatcatc acagtcagca gctacattgc
    27781 gcatttgcta aaagacgcgc cttgtgaagt cattttgctc ggtggcgtgt accagaaaaa
    27841 aagcgaaagt atggttggcc ctttgacacg ccagtgcatc cagcaggtac atttcagcaa
    27901 agcatttatt ggtattgatg gctggcaacc tgaaactgga ttcaccggtc gcgatatgat
    27961 gcgtaccgat gtggtcaatg ccgtgctgga aaaagagtgc gaagcgatag tcctgactga
    28021 cagctcgaaa tttggtgctg tacattcata ctccatcggt cccgttgagc gattcaatcg
    28081 cgtgattacc gattcgaaaa tacgcgccag cgatctgatg catcttgagc acagtaaact
    28141 caccgttcac gtcgttgaca tttaaccatt tcccttctct acggatgatt tgcagtttgg
    28201 caaatcatcc gctctaagat gattcctggt tgataattaa gactatttac ctgttattaa
    28261 cacgcttaag atataaaatt attatcagcg atataacagg aagtcattat cacctgcgtg
    28321 atataaccct gcgcgcgagc agatttcacg gaataatttc accaggctta ttcttagcta
    28381 ttatagttat agagagctta cttccgtgaa tcataaattc aggagagagt attatgtttg
    28441 taacgagcaa aaaaatgacc gcggctgttc tggcaattac tttggcaatg tctctgagtg
    28501 cctgttctaa ctggtctaaa cgggaccgca acaccgcaat cggcgcgggt gcaggggcat
    28561 taggcggtgc agtactgacc gatggcagta cgttgggtac attaggtggt gcagctgtcg
    28621 gtggtgttat tggtcaccag gtcggtaaat aatcgtaaat aaatgtcgat tatcgactcg
    28681 caattataat acgcttgatt ctgttcagta cgaaataact gaataataca cacagtaaaa
    28741 tattcgaaag ccacggatat atccgtggct ttttatgtta accgcctgcg agttttacct
    28801 tcatcccttt cgcttcaagt agtgatttta ataaatcacg cttatcgccc tggatttcaa
    28861 taactccatc tttaactgct ccgccgcagc cgcatttttt cttcagttcc gctgcaagtt
    28921 ttgtcagttc ggcatcatcg agatcgacac cggtaatcag gcaaacgccc ttacctttac
    28981 gtccactggt ctgacgctga atacgcacca caccgtcgcc tttagggcgt acaggggccg
    29041 ctttgggctc atcaatacgt ccggtttccg ttgagtagac cagacggctg ttggaatcac
    29101 tcatcatgca ctccgctgta aagaggcgtt gatcgctttc agcgtctgcg ctggatctac
    29161 cgattgcgtt accgggcgac caatcaccat ataatcaaca ccagccgaca acgcctgttc
    29221 tggcgtcata atgcggcgct ggtcaccagc ttcactcccc tgcggacgaa tgcccggcgt
    29281 aaccagtttg aactcctgac cgaatacctg tttaaagcgc acagcttcct gagcagaaca
    29341 caccacacca tcaaggccac atttttgcgt cagtgccgcc agacgttctg cataatctgc
    29401 aggtgacagt gtcatgccaa gatcgaccag gtcgctggct tccatgctgg tcaacactgt
    29461 cacagcaatc aaaagcggtg catctttgcc aaacggaacc agtgcctcac gcgctgcggt
    29521 catcatacgc gccccaccag aggcatgaac attcaccatc cacacgccta agtcagctgc
    29581 agcagcgaca gcgtgcgcag cagtgttagg aatatcgtgg aatttcaggt caagaaagat
    29641 atcaaaacca cgctgttgaa gttcgcgcac aaactgtggc ccaaacaatg taaacatctc
    29701 tttgccgacc ttcagacgac aatcgcgtgg gtcgatcttg tcgacaaagg acagcgcgtc
    29761 atcacgatta tgataatcaa gggcaacaac cacaggagaa ttcgtaacag cgcgggaaga
    29821 agatgaagca gttaacgtca tgaccagacc ttcttgatga tgggcaccgg aagtggcgcg
    29881 aaacaggtaa acggcgagca ttctacccgt ggaaataaaa aattaacagt tgcgattttt
    29941 cccctggcag gctttttctg cgccgtcgtt gccggagcac aatggaacat caataactag
    30001 tatgttgtaa ctaaagtagg attttttaaa aattacaggc catcaagacc gcgaatcggt
    30061 ttaatggttg accaggcccg acaagacgga caatgccagt agagggtgta tgcggtaaaa
    30121 ccacatttct ggcagcgata acgaggctta ctacgtacct tttcgccaac catgtcacgc
    30181 agcaccatca ggctctcttt ggcacgccct tcttccgctt catttaagtg gtaatccatt
    30241 aacttatgga acacacgcat ggtcggatga cgctgaagct ggcgcgtaat atagacctgt
    30301 gcggcctcac taccgtcgcg cgcttcgatg atatcagcaa gcatcaattc agcatcggca
    30361 ccggtgttct cttccaccgc gcgttgcagg aattctgccc attcggcagt tttacccaac
    30421 tgctgatagc aggtttgcaa catttccagc gtttcgctga ccagttctct gtcctgggat
    30481 atcacgcgtt gcaaactttc gacggctttg gcgtattctc ctttcgccat aaacacgcgt
    30541 cccatcatga tggatacgcg ggcgctgttt ttatctgccg ccgccccttt ttttagcaag
    30601 gtcatggcac gatcgagatc gtcgctggcc atatgctgca gggctaactc acagtagaaa
    30661 tgggcaattt cgacgcgctg tttatcttta cccagcttca ccaggcgttc ggcaacatca
    30721 attgctttct gccactcact ggtagcctgg tagatttgta gcaactgttg cagagcgcca
    30781 atgcggaagt cagtttcatc ggtcagctga ttgaacatgt cttccgcgcg gtcgtataac
    30841 ccggcggcca tgtaatcacg ccctagttgt tgaatcgcca acagacgctg ttcataggtc
    30901 agcgaggcgc tttccattag ggtctgatgg atgcgaatag cgcgatcaac ttcgccacgc
    30961 gaacggaaca ggtttccgag cgtaaggtgg gcttcaacgg tgcctgtatc ctctttaagc
    31021 atatcgagaa acaggtctac cgctttatcc tgttgattac taagcaggaa gttaaccccc
    31081 gctacgtaat cacgcgacaa gcggttggct tcatcttgct tgttttgttg cgcacttctg
    31141 cggcccatat accagccata ggcagcggct acaggcaaaa gcagaaacaa caactccagc
    31201 atagaaagtt attccttcgc cgctgacgag tgcggcacta cagccacgtc agtcgcgggt
    31261 gaaagctggt tttccagtcg ctttatttta cgttcagcgc gcgccaggga aacacgaact
    31321 cgcagccaga acaggccaca aatcaaccaa ccgatagcaa accccgcagc aaacaatacc
    31381 gccagcaatg tggaaatacg gtactcccct tgcgctaaca gataattaaa cgtcacctgt
    31441 tgatcgttct gcgcacccaa cgtgaccgaa atcacgaaga tcgctaacac cagtaagaaa
    31501 atgagtaaat atttcacatt acttcccgtt atgtggttag agcgaataaa ttatggtcaa
    31561 cttaccgcaa tagcccttta aagtaccatt ttcgcgccgt ctgcgaaatg gcatccacgc
    31621 gcattgttga tgatttaagg gctaaattcg gaaaatcaac cagcgttaac tttcttgttc
    31681 tcgttgcgct atttcgcgat tttcttccgc aggtggtgtt aatggcccac aaattcgttg
    31741 cgcaagccac gttgccaccg ccaccagcgc ccacgaaatc aacgtagcta ctaccagatc
    31801 gcgtggccaa tgcatcccga gcagcaggcg gcttcccatg actcccgttg cccagaccag
    31861 caagatagca atggttaacg ttcgccgacg cggccacagc aaaccaacgg ccagcagtgc
    31921 ccaactggca gcaaacatcg tgtgaccgga aggaaaggca aaccccgtct ctttctgcca
    31981 atgtgaacgc aaatattgtg ggatattttt ctcttcagcc aactgttctt tcactagatt
    32041 tccgcgttct gctcgcttta aagtgtagaa ctcatcaacc ggaatatgat gtgttttttc
    32101 cagccagata acaaaaggtc gtggttcctg gactttgtct ttgatccagg atttaacgcc
    32161 ttgtcccaca aggattgcgg ccgccagaat ggcaaataat acaaaggcag ccttaatgcg
    32221 aaaacgcaga caccagagaa accagccgaa taaaatcaaa tgtgtaatga cgccccaggg
    32281 ctgggtgaca gtttcagtaa cccaaaaagc cgcttttagt agccaacttt gttctccagg
    32341 ttgccaacgc cagccagaaa tccatacggc tactggcatg acaagcaata gtgcagctcc
    32401 cactgcggta cgtctggcaa tcgaacgcat ggcctctcct ttttgataag tcccacaatc
    32461 ataactgaaa acgccagttc caggaaaaat tgacagattt gtgccattcc gtgaacgatc
    32521 gacgcgtcgt gattaggtga accccttctc gttatggcaa aataagccaa tacagaacca
    32581 gcattatctg gagaatttca tgcagcttaa acgtgtggca gaagccaaac tgccaacccc
    32641 atggggcgat ttcctgatgg tgggatttga agaactggca accggacacg atcatgtcgc
    32701 gctagtctat ggcgatattt ccgggcatac cccggtactt gcgcgcgtcc attccgaatg
    32761 tctgaccggt gacgccctgt tcagcttgcg ctgcgattgt ggcttccagc tcgaagcggc
    32821 attgacgcaa attgccgagg aaggccgtgg tattttgctg tatcaccgtc aggaaggtcg
    32881 taacattggt ctgctgaata aaatccgcgc ttacgcactg caggatcaag gttacgatac
    32941 cgtagaggct aaccaccagt taggcttcgc cgctgatgag cgcgacttca ctctttgcgc
    33001 tgatatgttc aaactccttg gcgtcaatga agtccgcttg ttaaccaata acccgaaaaa
    33061 agtcgaaatt ctgaccgaag cagggattaa tattgttgaa cgcgtaccat tgattgtagg
    33121 tcgtaacccc aataacgaac attatctcga taccaaagcc gagaaaatgg gccatttgct
    33181 gaacaaataa ccctcttgca ttgtgtaatt aatttgcttg ccggaagcaa aataaccggc
    33241 aagcaaatac ttgttacttc aacatattac gaatgacata atgcaaaatg ccgtcgttct
    33301 ggtagtaggt caactccgtc gcggtgtcga tacgacaacg gcagggtacg acctcctggc
    33361 taccatccgc gcgcgtaagc gtcaccggaa ccgtcgcacc gggttgtaga ttttgcagat
    33421 cgccaatatc aatcttctct tccccggtta gtcctaacgt tttacgcgtt acgccttgcg
    33481 gaaattccag cggcaggatg cccatgccaa ttaaattcga acggtgaatt cgttcaaacg
    33541 attcggcaat caccacacga attccaagca gacgaggacc ttttgccgcc cagtcacggc
    33601 ttgagcctga tccatactct ttcccggcaa tcaccgccag cggcgtttgt tccttcttat
    33661 agcgcatcgc agcatcataa atagagacta cgtcgctgtc aggtaaatgc cgcgtcatcc
    33721 ccccttcaac gccaggcacc atttcattac ggatgcgaat attggcgaag gtgccgcgca
    33781 tcatcacttc atggttacca cgccgcgaac cgtaggagtt aaagtctttt cgctcaacac
    33841 cccgaccttg tagatatcga cccgctgggc tgtcgggctt aatactgccc gccggagaga
    33901 tatggtcagt ggtgactgaa tcccccagca ttgcgaggat ccgcgcaccg tgaatatctt
    33961 ccactggtgc tggtgttgcc tgcatttcat caaagaaagg cgataagcga atataggttg
    34021 agtcctcctg ccaaccgtag gtatcggatc gtgtgacgtt aatttccttc cactctgctg
    34081 tgccttcaaa aacttctgcg tactctttgc ggaacatttc tgtggagact tgttctaccg
    34141 cacgggcaat ttcttgtgcc gatggccaga tatctttcag ataaaccggg tcgcctttac
    34201 gatcatgacc aataggctca gcagccaggt tgatattcat atttcccgcc agcgcatagg
    34261 caaccaccag cggcggcgag gccagccagt tagttttaac cagcggatgg atacggcctt
    34321 caaagttacg gttgccggac agcaccgcac cgacggttaa atcgcctttt ttgattgccg
    34381 tttcgatagg atcgggcagc ggcccagagt taccaataca ggtggtacaa ccgtatccca
    34441 caaggttaaa ccccagttcg tcgagatacg gtgtcagttt cgcttttgcc agataatcag
    34501 aaacgacttt cgaacccggt gccagcgacg ctttgaccca cggttgccgc ttgaggccca
    34561 gagttacggc ttttttcgcc agcaagcctg cggccatcag cacacttggg ttagaggtgt
    34621 tggtgcacga ggttatcgca gcaatgacca cagcgccatc aggtaactga tactgatgtc
    34681 cgttcataac ataatcgacc ggctggcgat ctttatgcgt ggcattcact tccagttcgt
    34741 tactggcggc aaatgctttt ggtacatcgg gcagtgcaac gcgatcctgt gggcgttttg
    34801 gccctgccag gctcgcttca acgtcattca tatccagttc taacgtactg gtaaaaatcg
    34861 gttcatcgcc cgggttacgc cacatgccct gcgctttggc atatttttcg accaactcaa
    34921 cctgatcttc gctgcgcccg cttaaacgca tgtaatcgag ggttacagca tcgattggga
    34981 agaagccaca ggtggcacca tattctggcg acatattggc aatggtggcg cgatccgcca
    35041 gcggtagtga atccagacca tcaccataaa attcgacgaa tttccccacc acgccatgtt
    35101 tgcgcagcat ttgggtaaca gtgagaacca ggtctgtggc ggtaatacct tcacgtaatt
    35161 taccggtaag tttaaagccc actacgtccg ggataagcat ggaaaccggc tggccta
//
LOCUS       DAZKHF020000018        35029 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2201S31241
            SAMN43267800-rid25106413.denovo.018, whole genome shotgun sequence.
ACCESSION   DAZKHF020000018 DAZKHF020000000
VERSION     DAZKHF020000018.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267800
            Sequence Read Archive: SRR30307263
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 35029)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 35029)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 35029)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350581.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350581.2
            Genome Coverage       :: 151x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:21:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,652
            CDSs (total)                      :: 5,557
            Genes (coding)                    :: 5,320
            CDSs (with protein)               :: 5,320
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 237
            CDSs (without protein)            :: 237
            Pseudo Genes (ambiguous residues) :: 0 of 237
            Pseudo Genes (frameshifted)       :: 102 of 237
            Pseudo Genes (incomplete)         :: 130 of 237
            Pseudo Genes (internal stop)      :: 52 of 237
            Pseudo Genes (multiple problems)  :: 41 of 237
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..35029
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267800-rid25106413.denovo.018"
                     /serotype="O 26:H11"
                     /isolate="2201S31241"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-01"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            177..1271
                     /gene="gcvT"
                     /locus_tag="ACHOSC_RS03805"
                     /old_locus_tag="pgaptmp_000761"
     CDS             177..1271
                     /gene="gcvT"
                     /locus_tag="ACHOSC_RS03805"
                     /old_locus_tag="pgaptmp_000761"
                     /EC_number="2.1.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004047 - aminomethyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0019464 - glycine decarboxylation via
                     glycine cleavage system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system aminomethyltransferase
                     GcvT"
                     /protein_id="HGU4546751.1"
                     /translation="MAQQTPLYEQHTLCGARMVDFHGWMMPLHYGSQIDEHHAVRTDA
                     GMFDVSHMTIVDLRGSRTREFLRYLLANDVAKLTKSGKALYSGMLNASGGVIDDLIVY
                     YFTEDFFRLVVNSATREKDLSWITQHAEPFGIEITVRDDLSMIAVQGPNAQAKAATLF
                     NDAQRQAVEGMKPFFGVQAGDLFIATTGYTGEAGYEIALPNEKAADFWRALVEAGVKP
                     CGLGARDTLRLEAGMNLYGQEMDETISPLAANMGWTIAWEPADRDFIGREALEVQREH
                     GTEKLVGLVMTEKGVLRNELPVRFTDAQGNQHEGIITSGTFSPTLGYSIALARVPEGI
                     GETAIVQIRNREMPVKVTKPVFVRNGKAVA"
     gene            1295..1684
                     /gene="gcvH"
                     /locus_tag="ACHOSC_RS03810"
                     /old_locus_tag="pgaptmp_000762"
     CDS             1295..1684
                     /gene="gcvH"
                     /locus_tag="ACHOSC_RS03810"
                     /old_locus_tag="pgaptmp_000762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005960 - glycine cleavage complex
                     [Evidence IEA];
                     GO_process: GO:0019464 - glycine decarboxylation via
                     glycine cleavage system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system protein GcvH"
                     /protein_id="HGU4546752.1"
                     /translation="MSNVPAELKYSKEHEWLRKEADGTYTVGITEHAQELLGDMVFVD
                     LPEVGATVSAGDDCAVAESVKAASDIYAPVSGEIVAVNDALSDSPELVNSEPYAGGWI
                     FKIKASDESELESLLDATAYEALLEDE"
     gene            1803..4676
                     /gene="gcvP"
                     /locus_tag="ACHOSC_RS03815"
                     /old_locus_tag="pgaptmp_000763"
     CDS             1803..4676
                     /gene="gcvP"
                     /locus_tag="ACHOSC_RS03815"
                     /old_locus_tag="pgaptmp_000763"
                     /EC_number="1.4.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004375 - glycine dehydrogenase
                     (decarboxylating) activity [Evidence IEA];
                     GO_process: GO:0019464 - glycine decarboxylation via
                     glycine cleavage system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminomethyl-transferring glycine dehydrogenase"
                     /protein_id="HGU4546753.1"
                     /translation="MTQTLSQLENSGAFIERHIGPDAAQQQEMLNAVGAQSLNALTGQ
                     IVPKDIQLATPPQVGAPATEYAALAELKAIASRNKRFTSYIGMGYTAVQLPPVILRNM
                     LENPGWYTAYTPYQPEVSQGRLEALLNFQQVTLDLTGLDMASASLLDEATAAAEAMAM
                     AKRVSKLKNANRFFVASDVHPQTLDVVRTRAETFGFEVIVDDAQKVLDHQDVFGVLLQ
                     QVGTTGEIHDYTALISELKSRKIVVSVAADIMALVLLTAPGKQGADIVFGSAQRFGVP
                     MGYGGPHAAFFAAKDEYKRSMPGRIIGVSKDAAGNTALRMAMQTREQHIRREKANSNI
                     CTSQVLLANIASLYAVYHGPIGLKRIANRIHRLTDILAAGLQQKGLKLRHAHYFDTLC
                     VEVADKAGVLTRAEAAEINLRSDILNAVGITLDETTTRENVMQLFSVLLGDNHGLDID
                     TLDKDVAHDSRSIQPAMLRDDEILTHPVFNRYHSETEMMRYMHSLERKDLALNQAMIP
                     LGSCTMKLNAAAEMIPITWPEFAELHPFCPPEQAEGYQQMIAQLADWLVKLTGYDAVC
                     MQPNSGAQGEYAGLLAIRHYHESRNEGHRDICLIPASAHGTNPASAHMAGMQVVVVAC
                     DKNGNIDLTDLRAKAEQAGDNLSCIMVTYPSTHGVYEETIREVCEVVHQFGGQVYLDG
                     ANMNAQVGITSPGFIGADVSHLNLHKTFCIPHGGGGPGMGPIGVKAHLAPFVPGHSVV
                     QIEGMLTRQGAVSAAPFGSASILPISWMYIRMMGAEGLKKASQVAILNANYIASRLQD
                     AFPVLYTGRDGRVAHECILDIRPLKEETGISELDIAKRLIDYGFHAPTMSFPVAGTLM
                     VEPTESESKVELDRFIDAMLAIRAEIDQVKAGVWPLEDNPLVNAPHIQNELVAEWAHP
                     YSREVAVFPAGVADKYWPTVKRLDDVYGDRNLFCSCVPISEYQ"
     gene            4937..5680
                     /gene="ygfF"
                     /locus_tag="ACHOSC_RS03820"
                     /old_locus_tag="pgaptmp_000764"
     CDS             4937..5680
                     /gene="ygfF"
                     /locus_tag="ACHOSC_RS03820"
                     /old_locus_tag="pgaptmp_000764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HGU4546754.1"
                     /translation="MAIALVTGGSRGIGRATALLLAQEGYTVAVNYQQNLHAAQEVMN
                     LITQAGGKAFVLQADISDENQVVAMFTAIDQHDEPLAALVNNAGILFTQCTVENLTAE
                     RINRVLSTNVTGYFLCCREAVKRMALKNGGSGGAIVNVSSVASRLGSPGEYVDYAASK
                     GAIDTLTTGLSLEVAAQGIRVNCVRPGFIYTEMHASGGEPGRVDRVKSNIPMQRGGQA
                     EEVAQAIVWLLSDKASYVTGSFIDLAGGK"
     gene            complement(5737..7170)
                     /gene="bglA"
                     /locus_tag="ACHOSC_RS03825"
                     /old_locus_tag="pgaptmp_000765"
     CDS             complement(5737..7170)
                     /gene="bglA"
                     /locus_tag="ACHOSC_RS03825"
                     /old_locus_tag="pgaptmp_000765"
                     /EC_number="3.2.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708664.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase BglA"
                     /protein_id="HGU4546755.1"
                     /translation="MKKLTLPKDFLWGGAVAAHQVEGGWNKGGKGPSICDVLTGGAHG
                     VPREITKEVLPGKYYPNHEAVDFYGHYKEDIKLFAEMGFKCFRTSIAWTRIFPKGDEA
                     QPNEEGLKFYDDMFDELLKYNIEPVITLSHFEMPLHLVQQYGSWTNRKVVDFFVRFAE
                     VVFERYKHKVKYWMTFNEINNQRNWRAPLFGYCCSGVVYTEHENPEETMYQVLHHQFV
                     ASALAVKAARRIHPEMKVGCMLAMVPLYPYCCNPDDVMFAQESMRERYVFTDVQLRGY
                     YPSYVLNEWERRGFNIKMEDGDLDVLREGTCDYLGFSYYMTNAVKAEGGTGDAISGFE
                     GSVPNPYVKASDWGWQIDPVGLRYALCELYERYQRPLFIVENGFGAYDKVEEDGSIND
                     DYRIDYLRAHIEEMKKAVTYDGVDLMGYTPWGCIDCVSFTTGQYSKRYGFIYVNKHDD
                     GAGDMSRSRKKSFNWYQEVIASNGEKL"
     gene            7215..7526
                     /gene="yqfB"
                     /locus_tag="ACHOSC_RS03830"
                     /old_locus_tag="pgaptmp_000766"
     CDS             7215..7526
                     /gene="yqfB"
                     /locus_tag="ACHOSC_RS03830"
                     /old_locus_tag="pgaptmp_000766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N(4)-acetylcytidine aminohydrolase"
                     /protein_id="HGU4546756.1"
                     /translation="MQPNDITFFQRFQDDILAGRKTITIRDESESHFKTGDVLRVGRF
                     EDDGYFCTIEVTATSTVTLDTLTEKHAEQENMTLTELIKVIADIYPGQTQFYVIEFKC
                     L"
     gene            7690..8349
                     /gene="yqfA"
                     /locus_tag="ACHOSC_RS03835"
                     /old_locus_tag="pgaptmp_000767"
     CDS             7690..8349
                     /gene="yqfA"
                     /locus_tag="ACHOSC_RS03835"
                     /old_locus_tag="pgaptmp_000767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0019835 - cytolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin III family protein"
                     /protein_id="HGU4546757.1"
                     /translation="MVQKPLIKQGYSLAEEIANSVSHGIGLVFGIVGLVLLLVQAVDL
                     NASATAITSYSLYGGSMILLFLASTLYHAIPHQRAKMWLKKFDHCAIYLLIAGTYTPF
                     LLVGLDSPLARGLMIVIWSLALLGILFKLTIAHRFKILSLVTYLAMGWLSLVVIYEMA
                     VKLAAGSVTLLAVGGVVYSLGVIFYVCKRIPYNHAIWHGFVLGGSVCHFLAIYLYIGQ
                     A"
     gene            complement(8545..9525)
                     /gene="ygfZ"
                     /locus_tag="ACHOSC_RS03840"
                     /old_locus_tag="pgaptmp_000768"
     CDS             complement(8545..9525)
                     /gene="ygfZ"
                     /locus_tag="ACHOSC_RS03840"
                     /old_locus_tag="pgaptmp_000768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005542 - folic acid binding [Evidence
                     IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA-modifying protein YgfZ"
                     /protein_id="HGU4546758.1"
                     /translation="MAFTPFPPRQPTASARLPLTLMTLDDWALATITGADSEKYMQGQ
                     VTADVSQMTENQHLLAAHCDAKGKMWSNLRLFRDGDGFAWIERRSVREPQLTELKKYA
                     VFSKVTIAPDDERVLLGVAGFQARAALANLFSELPSKEKQVVKEGATTLLWFEHPAER
                     FLIVTDEATANMLTDKLRGEAELNNSQQWLALNIEAGFPVIDAANSGQFIPQATNLQA
                     LGGISFKKGCYTGQEMVARAKFRGANKRALWLLAGSASRLPEAGEDLELKMGENWRRT
                     GTVLAAVKLEDGQVVVQVVMNNDMEPDSIFRVRDDANTLHIEPLPYSLEE"
     gene            9768..10034
                     /gene="sdhE"
                     /locus_tag="ACHOSC_RS03845"
                     /old_locus_tag="pgaptmp_000769"
     CDS             9768..10034
                     /gene="sdhE"
                     /locus_tag="ACHOSC_RS03845"
                     /old_locus_tag="pgaptmp_000769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000354044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD assembly factor SdhE"
                     /protein_id="HGU4546759.1"
                     /translation="MDINNKARIHWACRRGMRELDISIMPFFEHEYDSLSDDEKRIFI
                     RLLECDDLDLFNWLMNHGKPADAELEMMVRLIQTRNRERGPVAI"
     gene            10015..10422
                     /gene="ygfX"
                     /locus_tag="ACHOSC_RS03850"
                     /old_locus_tag="pgaptmp_000770"
     CDS             10015..10422
                     /gene="ygfX"
                     /locus_tag="ACHOSC_RS03850"
                     /old_locus_tag="pgaptmp_000770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YgfX"
                     /protein_id="HGU4546760.1"
                     /translation="MVLWQSDLRVSWRAQWLSLLIHGLVAAVILLMPWPLSYTPLWMV
                     LLSLVVFDCVRSQRRINARQGEIRLLMDGRLRWQGQEWSIVKAPWMIKSGMMLRLRSD
                     GGKRQHLWLAADSMDEAEWRDLRRILLQQETQR"
     gene            complement(10462..10983)
                     /gene="fldB"
                     /locus_tag="ACHOSC_RS03855"
                     /old_locus_tag="pgaptmp_000771"
     CDS             complement(10462..10983)
                     /gene="fldB"
                     /locus_tag="ACHOSC_RS03855"
                     /old_locus_tag="pgaptmp_000771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin FldB"
                     /protein_id="HGU4546761.1"
                     /translation="MNMGLFYGSSTCYTEMAAEKIRDIIGPELVTLHNLKDDSPKLME
                     QYDVLILGIPTWDFGEIQEDWEAVWDQLDDLNLEGKIVALYGLGDQLGYGEWFLDALG
                     MLHDKLSTKGVKFVGYWPTEGYEFTSPKPVIADGQLFVGLALDETNQYDLSDERIQSW
                     CEQILNEMAEHYA"
     gene            11095..11991
                     /gene="xerD"
                     /locus_tag="ACHOSC_RS03860"
                     /old_locus_tag="pgaptmp_000772"
     CDS             11095..11991
                     /gene="xerD"
                     /locus_tag="ACHOSC_RS03860"
                     /old_locus_tag="pgaptmp_000772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009009 - site-specific recombinase
                     activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific tyrosine recombinase XerD"
                     /protein_id="HGU4546762.1"
                     /translation="MKQDLARIEQFLDALWLEKNLAENTLNAYRRDLSMMVEWLHHRG
                     LTLATAQSDDLQALLAERLEGGYKATSSARLLSAVRRLFQYLYREKFREDDPSAHLAS
                     PKLPQRLPKDLSEAQVERLLQAPLIDQPLELRDKAMLEVLYATGLRVSELVGLTMSDI
                     SLRQGVVRVIGKGNKERLVPLGEEAVYWLETYLEHGRPWLLNGVSIDVLFPSQRAQQM
                     TRQTFWHRIKHYAVLAGIDSEKLSPHVLRHAFATHLLNHGADLRVVQMLLGHSDLSTT
                     QIYTHVATERLRQLHQQHHPRA"
     gene            12016..12726
                     /gene="dsbC"
                     /locus_tag="ACHOSC_RS03865"
                     /old_locus_tag="pgaptmp_000773"
     CDS             12016..12726
                     /gene="dsbC"
                     /locus_tag="ACHOSC_RS03865"
                     /old_locus_tag="pgaptmp_000773"
                     /EC_number="5.3.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional protein-disulfide
                     isomerase/oxidoreductase DsbC"
                     /protein_id="HGU4546763.1"
                     /translation="MKKGFMLFTLLAAFSGFAQADDAAIQQTLAKMGIKSSDIQPAPV
                     AGMKTVLTNSGVLYITDDGKHIIQGPMYDVSGTAPVNVTNKMLLKQLNALEKEMIVYK
                     APQEKHVITVFTDITCGYCHKLHEQMADYNALGITVRYLAFPRQGLDSDAEKEMKAIW
                     CAKDKNKAFDDVMAGKSVAPASCDVDIADHYALGVQLGVSGTPAVVLSNGTLVPGYQP
                     PKEMKEFLDEHQKMTSGK"
     gene            12732..14465
                     /gene="recJ"
                     /locus_tag="ACHOSC_RS03870"
                     /old_locus_tag="pgaptmp_000774"
     CDS             12732..14465
                     /gene="recJ"
                     /locus_tag="ACHOSC_RS03870"
                     /old_locus_tag="pgaptmp_000774"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008409 - 5'-3' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded-DNA-specific exonuclease RecJ"
                     /protein_id="HGU4546764.1"
                     /translation="MKQQIQLRRREVDETADLPAELPPLLRRLYASRGVRSAQELERS
                     VKGMLPWQQLSGVEKAVEILYNAFREGTRIIVVGDFDADGATSTALSVLAMRSLGCSN
                     IDYLVPNRFEDGYGLSPEVVDQAHARGAQLIVTVDNGISSHAGVVHARSLGIPVIVTD
                     HHLPGETLPAAEAIINPNLRDCNFPSKSLAGVGVAFYLMLALRTFLRDQGWFDERGIA
                     IPNLAELLDLVALGTVADVVPLDANNRILTWQGMSRIRAGKCRPGIKALLEVANRDAQ
                     KLAASDLGFALGPRLNAAGRLDDMSVGVALLLCDNIGEARVLANELDALNQTRKEIEQ
                     GMQVEALTLCEKLERSRDTLPGGLAMYHPEWHQGVVGILASRIKERFHRPVIAFAPAG
                     DGTLKGSGRSIQGLHMRDALERLDTLYPGMILKFGGHAMAAGLSLEEDKFELFQQRFG
                     ELVTEWLDPSLLQGEVVSDGPLSPAEMTMEVAQLLRDAGPWGQMFPEPLFDGHFRLLQ
                     QRLVGERHLKVMVEPVGGGPLLDGIAFNVDTALWPDNGVREVQLAYKLDINEFRGNRS
                     LQIIIDNIWPI"
     gene            14556..15654
                     /gene="prfB"
                     /locus_tag="ACHOSC_RS03875"
                     /old_locus_tag="pgaptmp_000775"
     CDS             join(14556..14630,14632..15654)
                     /gene="prfB"
                     /locus_tag="ACHOSC_RS03875"
                     /old_locus_tag="pgaptmp_000775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311790.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0016149 - translation release factor
                     activity, codon specific [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 2"
                     /protein_id="HGU4546765.1"
                     /translation="MFEINPVNNRIQDLTERSDVLRGYLDYDAKKERLEEVNAELEQP
                     DVWNEPERAQALGKERSSLEAVVDTLDQMKQGLEDVSGLLELAVEADDEETFNEAVAE
                     LDALEEKLAQLEFRRMFSGEYDSADCYLDIQAGSGGTEAQDWASMLERMYLRWAESRG
                     FKTEIIEESEGEVAGIKSVTIKISGDYAYGWLRTETGVHRLVRKSPFDSGGRRHTSFS
                     SAFVYPEVDDDIDIEINPADLRIDVYRASGAGGQHVNRTESAVRITHIPTGIVTQCQN
                     DRSQHKNKDQAMKQMKAKLYELEMQKKNAEKQAMEDNKSDIGWGSQIRSYVLDDSRIK
                     DLRTGVETRNTQAVLDGSLDQFIEASLKAGL"
     gene            15664..17181
                     /gene="lysS"
                     /locus_tag="ACHOSC_RS03880"
                     /old_locus_tag="pgaptmp_000776"
     CDS             15664..17181
                     /gene="lysS"
                     /locus_tag="ACHOSC_RS03880"
                     /old_locus_tag="pgaptmp_000776"
                     /EC_number="6.1.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001740376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004824 - lysine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006430 - lysyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysine--tRNA ligase"
                     /protein_id="HGU4546766.1"
                     /translation="MSEQHAQGADAVVDLNNELKTRREKLANLREQGVAFPNDFRRDH
                     TSDQLHAEFDGKENEELEALNIEVAVAGRMMTRRIMGKASFVTLQDVGGRIQLYVARD
                     DLPEGVYNEQFKKWDLGDILGAKGKLFKTKTGELSIHCTELRLLTKALRPLPDKFHGL
                     QDQEARYRQRYLDLISNDESRNTFKVRSQILSGIRQFMVNRGFMEVETPMMQVIPGGA
                     AARPFITHHNALDLDMYLRIAPELYLKRLVVGGFERVFEINRNFRNEGISVRHNPEFT
                     MMELYMAYADYKDLIELTESLFRTLAQDILGKTEVTYGDVTLDFGKPFEKLTMREAIK
                     KYRPETDMADLDNFDSAKAIAESIGIHVEKSWGLGRIVTEIFEEVAEAHLIQPTFITE
                     YPAEVSPLARRNDINPEITDRFEFFIGGREIGNGFSELNDAEDQAQRFLDQVAAKDAG
                     DDEAMFYDEDYVTALEHGLPPTAGLGIGIDRMVMLFTNSHTIRDVILFPAMRPVK"
     gene            complement(17224..17772)
                     /gene="idi"
                     /locus_tag="ACHOSC_RS03885"
                     /old_locus_tag="pgaptmp_000777"
     CDS             complement(17224..17772)
                     /gene="idi"
                     /locus_tag="ACHOSC_RS03885"
                     /old_locus_tag="pgaptmp_000777"
                     /EC_number="5.3.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004452 - isopentenyl-diphosphate
                     delta-isomerase activity [Evidence IEA];
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isopentenyl-diphosphate Delta-isomerase"
                     /protein_id="HGU4546767.1"
                     /translation="MQTEHVILLNAQGVPTGTLEKYAAHTADTRLHRAFSSWLFNAKG
                     QLLVTRRALSKKAWPGVWTNSVCGHPQLGESNEDAVIRRCRYGLGVEITPPESIYPDF
                     RYRATDPNGIVENEVCPVFAARTTSALQINDDEVMDYQWCDLADVLHGIDATPWAFSP
                     WMVMQAANSEARKLLSAFAQHN"
     gene            17827..17898
                     /locus_tag="ACHOSC_RS03890"
                     /old_locus_tag="pgaptmp_000778"
     CDS             17827..17898
                     /locus_tag="ACHOSC_RS03890"
                     /old_locus_tag="pgaptmp_000778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4546768.1"
                     /translation="MINQVSVYRQPPVLSGCRQVKTI"
     gene            complement(17895..18020)
                     /gene="yqfG"
                     /locus_tag="ACHOSC_RS03895"
                     /old_locus_tag="pgaptmp_000779"
     CDS             complement(17895..18020)
                     /gene="yqfG"
                     /locus_tag="ACHOSC_RS03895"
                     /old_locus_tag="pgaptmp_000779"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YqfG"
                     /protein_id="HGU4546769.1"
                     /translation="MNFLMRAIFSLLLLFTLSIPVISDCVAMAIESRFKYMMLLF"
     gene            complement(18022..19470)
                     /gene="uacT"
                     /locus_tag="ACHOSC_RS03900"
                     /old_locus_tag="pgaptmp_000780"
     CDS             complement(18022..19470)
                     /gene="uacT"
                     /locus_tag="ACHOSC_RS03900"
                     /old_locus_tag="pgaptmp_000780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417364.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="urate/proton symporter UacT"
                     /protein_id="HGU4546770.1"
                     /translation="MSAIDSQLPSSSGQDRPTDEVDRILSPGKLIILGLQHVLVMYAG
                     AVAVPLMIGDRLGLSKEAIAMLISSDLFCCGIVTLLQCIGIGRFMGIRLPVIMSVTFA
                     AVTPMIAIGMNPDIGLLGIFGATIAAGFITTLLAPLIGRLMPLFPPLVTGVVITSIGL
                     SIIQVGIDWAAGGKGNPQYGNPVYLGISFAVLIFILLITRYAKGFMSNVAVLLGIVFG
                     FLLSWMMNEVNLSGLHDASWFAIVTPMSFGMPIFDPVSILTMTAVLIIVFIESMGMFL
                     ALGEIVGRKLSSHDIIRGLRVDGVGTMIGGTFNSFPHTSFSQNVGLVSVTRVHSRWVC
                     ISSGIILILFGMVPKMAVLVASIPQFVLGGAGLVMFGMVLATGIRILSRCNYTTNRYN
                     LYIVAISLGVGMTPTLSHDFFSKLPAVLQPLLHSGIMLATLSAVVLNVFFNGYQHHAD
                     LVKESVSDKDLKVRTVRMWLLMRKLKKNEHGE"
     gene            19906..21825
                     /gene="ygfT"
                     /locus_tag="ACHOSC_RS03905"
                     /old_locus_tag="pgaptmp_000781"
     CDS             19906..21825
                     /gene="ygfT"
                     /locus_tag="ACHOSC_RS03905"
                     /old_locus_tag="pgaptmp_000781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417363.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate-dependent uric acid utilization protein
                     YgfT"
                     /protein_id="HGU4546771.1"
                     /translation="MNKFIAAEAAECIGCHACEIACAVAHNQENWPLSHSDFRPRIHV
                     VGKGQAANPVACHHCNNAPCVTACPVNALTFQSDSVQLDEQKCIGCKRCAIACPFGVV
                     EMVDTIAQKCDLCNQRSSGTQACIEVCPTQALRLMDDKGLQWIKVARQRKTAAGKASS
                     DAQPSRSAALLPVNSRKGADKISASERKTHFGEIYCGLDPLQATYESDRCVYCAEKAN
                     CNWHCPLHNAIPDYIRLVQEGKIIEAAELCHQTSSLPEICGRVCPQDRLCEGACTLKD
                     HSGAVSIGNLERYITDTALAMGWRPDVSKVVPRSEKVAVIGAGPAGLGCADILARAGV
                     QVDVFDRHPEIGGMLTFGIPPFKLDKTVLSQRREIFTAMGIDFHLNCEIGRDITFSDL
                     TSEYDAVFIGVGTYGMMRADLPHEDAPGVIQALPFLTAHTRQLMGLPESEEYPLTDVE
                     GKRVVVLGGGDTTMDCLRTSIRLNAASVTCAYRRDEVSMPGSRKEVVNAREEGVEFQF
                     NVQPQYIACDEDGRLTAVGLIRTAMGEPGPDGRRRPRPVAGSEFELPADVLIMAFGFQ
                     AHAMPWLQGSGIKLDKWGLIQTGDVGYLPTQTHLKKVFAGGDAVHGADLVVTAMAAGR
                     QAARDMLTLFDTKAS"
     gene            21825..22313
                     /gene="ygfS"
                     /locus_tag="ACHOSC_RS03910"
                     /old_locus_tag="pgaptmp_000782"
     CDS             21825..22313
                     /gene="ygfS"
                     /locus_tag="ACHOSC_RS03910"
                     /old_locus_tag="pgaptmp_000782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417362.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0009061 - anaerobic respiration [Evidence
                     IEA];
                     GO_process: GO:0019645 - anaerobic electron transport
                     chain [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HGU4546772.1"
                     /translation="MKSLIIVNPADCIGCRTCEVACVVAHPSEQELNADVFLPRLKVQ
                     RLDSISAPVMCHQCENAPCVGACPVGALTMGEQVVQTNSARCIGCQSCVSACPFGMIT
                     IQSLPGDTRQQIVKCDLCEQREEGPACVESCPTQALQLLTERELRRVRQQRIVASGEN
                     PL"
     gene            complement(22349..23716)
                     /gene="ghxQ"
                     /locus_tag="ACHOSC_RS03915"
                     /old_locus_tag="pgaptmp_000783"
                     /gene_synonym="ygfQ"
     CDS             complement(22349..23716)
                     /gene="ghxQ"
                     /locus_tag="ACHOSC_RS03915"
                     /old_locus_tag="pgaptmp_000783"
                     /gene_synonym="ygfQ"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="guanine/hypoxanthine transporter GhxQ"
                     /protein_id="HGU4546773.1"
                     /translation="MSGDILQTPDAPKPQGALDNYFKITARGSTVRQEVLAGLTTFLA
                     MVYSVIVVPGMLGKAGFPPAAVFVATCLVAGFGSLLMGLWANLPMAIGCAISLTAFTA
                     FSLVLGQQISVPVALGAVFLMGVIFTAISVTGVRTWILRNLPMGIAHGTGIGIGLFLL
                     LIAANGVGMVIKNPIEGLPVALGAFTSFPVMMSLLGLAVIFGLEKCRVPGGILLVIIA
                     ISIIGLIFDPAVKYHGLVAMPSLTGEDGKSLIFSLDIMGALQPTVLPSVLALVMTAVF
                     DATGTIRAVAGQANLLDKDNQIINGGKALTSDSVSSIFSGLVGAAPAAVYIESAAGTA
                     AGGKTGLTATVVGALFLLILFLSPLSFLIPGYATAPALMYVGLLMLSNVSKLDFNDFI
                     DAMAGLVCAVFIVLTCNIVTGIMLGFVTLVVGRVFAREWQKLNIGTVIITAALVAFYA
                     GGWAI"
     gene            complement(23752..25068)
                     /gene="guaD"
                     /locus_tag="ACHOSC_RS03920"
                     /old_locus_tag="pgaptmp_000784"
     CDS             complement(23752..25068)
                     /gene="guaD"
                     /locus_tag="ACHOSC_RS03920"
                     /old_locus_tag="pgaptmp_000784"
                     /EC_number="3.5.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311783.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0008892 - guanine deaminase activity
                     [Evidence IEA];
                     GO_process: GO:0006147 - guanine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="guanine deaminase"
                     /protein_id="HGU4546774.1"
                     /translation="MSGEHTLKAVRGSFIDVTRTVDNPEEIASALRFIEDGLLLIKQG
                     KVEWFGEWEDGKHQIPDTIRVRDYRGKLIVPGFVDTHIHYPQSEMVGAYGEQLLEWLN
                     KHTFPTERRYEDLEYAREMSAFFIKQLLRNGTTTALVFGTVHPQSVDALFEAASHINM
                     RMIAGKVMMDRNAPDYLLDTAESSYHQSKELIERWHKNGRLLYAITPRFAPTSSPEQM
                     AMAQRLKEEYPDTWVHTHLCENKDEIAWVKSLYPDHDGYLDVYHQYGLTGKNCVFAHC
                     VHLEEKEWDRLSETKSSIAFCPTSNLYLGSGLFNLKKAWQKKVKVGMGTDIGAGTTFN
                     MLQTLNEAYKVLQLQGYRLSAYEAFYLATLGGAKSLGLDDLIGNFLPGKEADFVVMEP
                     TATPLQQLRYDNSVSLVDKLFVMMTLGDDRSIYRTYVDGRLVYERN"
     gene            complement(25086..26486)
                     /gene="xanQ"
                     /locus_tag="ACHOSC_RS03925"
                     /old_locus_tag="pgaptmp_000785"
     CDS             complement(25086..26486)
                     /gene="xanQ"
                     /locus_tag="ACHOSC_RS03925"
                     /old_locus_tag="pgaptmp_000785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417358.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine/proton symporter XanQ"
                     /protein_id="HGU4546775.1"
                     /translation="MSDINHAGSDLIFELEDRPPFHQALVGAITHLLAIFVPMVTPAL
                     IVGAALQLSAETTAYLVSMAMIASGIGTWLQVNRYGIVGSGLLSIQSVNFSFVTVMIA
                     LGSSMKSDGFHEELIMSSLLGVSFVGAFLVVGSSFILPYLRRVITPTVSGIVVLMIGL
                     SLIKVGIIDFGGGFAAKSSGTFGNYEHLGVGLLVLIVVIGFNCCRSPLLRMGGIAIGL
                     CVGYIASLCLGMVDFSSMRNLPLITIPHPFKYGFSFSFHQFLVVGTIYLLSVLEAVGD
                     ITATAMVSRRPIQGEEYQSRLKGGVLADGLVSVIASAVGSLPLTTFAQNNGVIQMTGV
                     ASRYVGRTIAVMLVILGLFPMIGGFFTTIPSAVLGGAMTLMFSMIAIAGIRIIITNGL
                     KRRETLIVATSLGLGLGVSYDPEIFKILPASIYVLVENPICAGGLTAILLNIILPGGY
                     RQENVLPGITSAEEMD"
     gene            complement(26651..29521)
                     /gene="xdhD"
                     /locus_tag="ACHOSC_RS03930"
                     /old_locus_tag="pgaptmp_000786"
     CDS             complement(26651..29521)
                     /gene="xdhD"
                     /locus_tag="ACHOSC_RS03930"
                     /old_locus_tag="pgaptmp_000786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase
                     Mo/Fe-S-binding subunit"
                     /protein_id="HGU4546776.1"
                     /translation="MIIHFTLNGAPQELTVNPGENVQKLLFNMGMHSVRNSDDGFGFA
                     GSDAIIFNGNIVNASLLIAAQLEKADIRTAESLGKWNELSLVQQAMVDVGVVQSGYND
                     PAAALIITDLLDRIAAPTREEIDDALSGLFSRDAGWQQYYQVIELAVARKNNPQATID
                     IAPTFRDDLEVIGKHYPKTDAAKMVQAKPCYVEDRVTADACVIKMLRSPHAHALITHL
                     DVSKAEALPGVVHVITHLNCPDIYYTPGGQSAPEPSPLDRRMFGKKMRHVGDRVAAVV
                     AESEEIALEALKLIDVEYEVLKPVMSIDEAMAEDAPVVHDEPVVYVAGAPDTLEDDNS
                     HAAQRGEHMIINFPIGSRPRKNIAASIHGHIGDMDKGFADADVIIERTYNSTQAQQCP
                     TETHICFTRMDGDRLVIHASTQVPWHLRRQVARLVGMKQHKVHVIKERVGGGFGSKQD
                     ILLEEVCAWATCVTGRPVLFRYTREEEFIANTSRHVAKVTVKLGAKKDGRLTAVKMDF
                     RANTGPYGNHSLTVPCNGPALSLPLYPCDNVDFQVTTYYSNICPNGAYQGYGAPKGNF
                     AITMALAELAEQLQIDQLEIIERNRVHEGQELKILGAIGEGKAPTSVPSAASCALEEI
                     LRQGREMIQWSSPKPQNSDWHIGRGVAIIMQKSGIPDIDQANCMIKLESDGTFIVHSG
                     GADIGTGLDTVVTKLAAEVLHCPPQDVHVISGDTDHALFDKGAYASSGTCFSGNAARL
                     AAENLREKILFHGAQMLGEPVADVQLATPGVVRGKKGEVSFGDIAHKGETGTGFGSLV
                     GTGSYITPDFAFPYGANFAEVAVNTRTGEIRLDKFYALLDCGTPVNPELALGQIYGAT
                     LRAIGHSMSEEIIYNAEGHPLTRDLRSYGAPKIGDIPRDFRAVLVPSDDKVGPFGAKS
                     ISEIGVNGAAPAIATAIHDACGIWLREWHFTPEKILTALEKI"
     gene            complement(29518..30297)
                     /gene="ygfM"
                     /locus_tag="ACHOSC_RS03935"
                     /old_locus_tag="pgaptmp_000787"
     CDS             complement(29518..30297)
                     /gene="ygfM"
                     /locus_tag="ACHOSC_RS03935"
                     /old_locus_tag="pgaptmp_000787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase
                     FAD-binding subunit"
                     /protein_id="HGU4546777.1"
                     /translation="MIEQFFRPDSVEQALELKRRYQDEAVWFAGGSKLNATPTRTDKK
                     IAISLQDLELDWVDWDNGALRIGAMSRLQPLRDARFIPAALREALGFVYSRHVRNQST
                     IGGEIAARQEESVLLPVLLALDAELVFGNGETLSIEDYLACPCDRLLTEIIIKDPYRT
                     CATRKISRSQAGLTVVTAAVAMTDHDGMRIALDGVASKALRLHDVEKQNLEGNALEQA
                     VANAIFPQEDLRGSVAYKRYITGVLVADLYADCQQAGEEAV"
     gene            complement(30348..31676)
                     /gene="ssnA"
                     /locus_tag="ACHOSC_RS03940"
                     /old_locus_tag="pgaptmp_000788"
     CDS             complement(30348..31676)
                     /gene="ssnA"
                     /locus_tag="ACHOSC_RS03940"
                     /old_locus_tag="pgaptmp_000788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative aminohydrolase SsnA"
                     /protein_id="HGU4546778.1"
                     /translation="MLILKNVTAVQLHPAKVQEGVDIAIENDVIVAIGDALTQRYPDA
                     SYKEMHGRIVMPGIVCSHNHFYSGLSRGIMANIAPCPDFISTLKNLWWRLDRALDEES
                     LYYSGLICSLEAIKSGCTSVIDHHASPAYIGGSLSTLRDAFLKVGLRAMTCFETTDRN
                     NGIKELQEGVEENIRFARQIDEAKKAATEPYLVEAHIGAHAPFTVPDAGLEMLREAVK
                     ATGRGLHIHAAEDLYDVSYSHHWYGKDLLARLAQFDLIDSKTLVAHGLYLSKDDIALL
                     NQRDAFLVHNARSNMNNHVGYNHHLSDIRNLALGTDGIGSDMFEEMKFAFFKHRDAGG
                     PLWPDSFAKALTNGNELMSRNFGAKFGLLEAGYKADLTICDYNSPTPLLADNIAGHIA
                     FGMGSGSVHSVMVNGVMVYEDRQFNFDCDSIYAQARKAAASMWRRMDALA"
     gene            complement(31679..34777)
                     /gene="ygfK"
                     /locus_tag="ACHOSC_RS03945"
                     /old_locus_tag="pgaptmp_000789"
     CDS             complement(31679..34777)
                     /gene="ygfK"
                     /locus_tag="ACHOSC_RS03945"
                     /old_locus_tag="pgaptmp_000789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative selenate reductase subunit YgfK"
                     /protein_id="HGU4546779.1"
                     /translation="MGDIMRPIPFEELLTRIFDEYQQQRSIFGIPEQQFYSPVKGKTV
                     SVFGETCATPVGPAAGPHTQLAQNIVTSWLTGGRFIELKTVQILDRLELEKPCIDAED
                     ECFNTEWSTEFTLLKAWDEYLKAWFALHLLEAMFQPSDSGKSFIFNMSVGYNLEGIKQ
                     PPMQQFIDNMMDASDHPKFAQYRDTLNKLLQDDAFLARHGLQEKRESLQALPARIPTS
                     MVQGVTLSTMHGCPPHEIEAICRYMLEEKGLNTFVKLNPTLLGYARVREILDVCGFGY
                     IGLKEESFDHDLKLTQALEMLERLMALAKEKSLGFGVKLTNTLGTINNKGALPGEEMY
                     MSGRALFPLSINVAAVLSRAFDGKLPISYSGGASQLTIRDIFDTGIRPITMATDLLKP
                     GGYLRLSACMRELEGSDAWGLDHVDVERLNRLAADALTMEYTQKHWKPEERIEVAEDL
                     PLTDCYVAPCVTACAIKQDIPEYIRLLGEHRYADALELIYQRNALPAITGHICDHQCQ
                     YNCTRLDYDSALNIRELKKVALEKGWDEYKQRWHKPAGSGSRHPVAVIGAGPAGLAAG
                     YFLARAGHPVTLFEREANAGGVVKNIIPQFRIPAELIQHDIDFVADHGVKFEYGCSPD
                     LTVEQLKNQGFHYVLIATGTDKNSGVKLAGDNQNVWKSLPFLREYNKGTALKLGKHVV
                     VVGAGNTAMDCARAALRVPGVEKATIVYRRSLQEMPAWREEYEEALHDGVEFRFLNNP
                     ERFDADGTLTLRVMSLGEPDEKGRRRPVETNETVTLHVDSLITAIGEQQDTEALNAMG
                     VPLDKNGWPDVDHNGETRLTDVFMIGDVQRGPSSIVAAVGTARRATDAILSRENIRSH
                     QNDKYWNNVNPAEIYQRKGDISITLVNSDDRDAFVAQEAARCLECNYVCSKCVDVCPN
                     RANVSIAVPGFQNRFQTLHLDAYCNECGNCAQFCPWNGKPYKDKITVFSLAQDFDNSS
                     NPGFLVEDCRVRVRLNNQSWVLNIDSEGQFNNVPPELNDMCRIISHVHQHHHYLLGRV
                     EV"
BASE COUNT         8592 a         8897 c         9281 g         8259 t
ORIGIN      
        1 accataagct aatgtgatga tcaattttac cttatggtta acagtctgtt tcggtggtaa
       61 gttcaggcaa aagagaacga ttgcgttggg gaccgggagt ggctccgatg ctgggtttcg
      121 tggtgataat ttcaccatga aaaagttgtc agccccgctt attcaatgag gacaagatgg
      181 cacaacagac tcctttgtac gaacaacaca cgctttgcgg cgctcgcatg gtggatttcc
      241 acggctggat gatgccgctg cattacggtt cgcaaatcga cgaacatcat gcggtacgta
      301 ccgatgccgg aatgtttgat gtgtcacata tgaccatcgt cgatcttcgc ggcagccgca
      361 cccgggagtt tctgcgttat ctgctggcga acgatgtggc gaagctcacc aaaagcggca
      421 aagcccttta ctcggggatg ttgaatgcct ctggcggtgt gatagatgac ctcatcgtct
      481 actactttac tgaagatttc ttccgcctcg ttgttaactc cgccacccgc gaaaaagacc
      541 tctcctggat tacccaacac gctgaacctt tcggcatcga aattaccgtt cgtgatgacc
      601 tttccatgat cgccgtacaa gggccgaatg cgcaggcaaa agctgccaca ctgtttaatg
      661 atgctcagcg tcaggcggtg gaagggatga aaccgttctt tggcgtgcag gcgggcgatc
      721 tgtttattgc caccactggt tataccggtg aagcgggcta tgaaattgcg ctgcccaatg
      781 aaaaagcggc cgatttctgg cgtgcgctgg tggaagcggg cgttaagcca tgcggtctgg
      841 gcgcgcgtga cacgctgcgt ctggaagcgg gcatgaatct ttatggtcag gagatggacg
      901 aaactatttc tcctttagcc gccaacatgg gctggactat cgcctgggaa ccggcagatc
      961 gtgacttcat cggtcgtgaa gccctggaag tgcagcgtga gcatggcaca gaaaaactgg
     1021 ttggtctggt gatgaccgaa aaaggcgtgc tgcgtaatga actgccggta cgttttaccg
     1081 atgcgcaggg caaccagcat gaaggcatta tcaccagcgg gactttctcc ccgacgctgg
     1141 gttacagcat tgcgctggca cgcgtgccgg aaggtattgg cgaaacggcg attgtgcaaa
     1201 ttcgcaaccg tgaaatgccg gttaaagtga cgaaacctgt ttttgtgcgt aacggtaaag
     1261 ccgtcgcgtg atttactttt ttggagattg attgatgagc aacgtaccag cagaactgaa
     1321 atacagcaaa gaacacgaat ggctgcgtaa agaagccgac ggcacttaca ccgttggtat
     1381 aaccgaacat gcccaggagc tgttaggcga tatggtgttt gttgacctgc cggaagtggg
     1441 cgcaacggtt agcgcgggcg atgactgcgc ggttgccgaa tcagtaaaag cggcgtcaga
     1501 catttatgcg ccagtaagcg gtgaaatcgt ggcggtaaac gacgcactga gcgattcccc
     1561 ggaactggtg aacagcgaac cgtatgcagg tggctggatc tttaaaatca aagccagcga
     1621 tgaaagcgaa ctggaatcac tgctggatgc gaccgcatac gaagcattgt tagaagacga
     1681 gtaacggctt tattcctctt ctgcgggaga ggatcagggt gaggaaaatt tatgcctcac
     1741 cctcactctc ttcgtaagga gagaggttca caattcactg cacgtttcag gaaccatcgc
     1801 tcatgacaca gacgttaagc cagcttgaaa acagcggcgc ttttattgaa cgccatatcg
     1861 gaccggacgc cgcgcaacag caagaaatgc tgaatgccgt tggtgcacaa tcgttaaacg
     1921 cgctgaccgg ccagattgtg ccgaaagata ttcagcttgc gaccccgccg caggttggcg
     1981 caccggcgac cgaatacgcc gcactggcag aactcaaggc tattgccagt cgcaataaac
     2041 gcttcacgtc ttacatcggc atgggttaca ccgccgtgca gctaccgccg gttatcctgc
     2101 gtaacatgct ggaaaatccg ggctggtata ccgcgtacac tccgtatcaa cctgaagtct
     2161 cccagggccg ccttgaagca ctgctcaact tccagcaggt aacgctggat ttgactggac
     2221 tggatatggc ctctgcttct cttctggacg aggccaccgc tgccgccgaa gcaatggcga
     2281 tggcgaaacg cgtcagcaaa ctgaaaaatg ccaaccgctt cttcgtggct tccgatgtgc
     2341 atccgcaaac gctggatgtg gtccgtactc gtgccgaaac ctttggtttt gaagtgattg
     2401 tcgatgacgc gcaaaaagtg ctcgaccatc aggacgtctt cggcgtgctg ttacagcagg
     2461 ttggcacaac cggtgaaatc cacgactaca ccgcgcttat tagcgaactg aaatcacgca
     2521 aaattgtggt cagtgtcgcc gccgatatta tggcgctggt gctgttaact gcgccgggta
     2581 aacagggcgc ggatattgtt tttggttcgg cgcaacgctt cggcgtgccg atgggctacg
     2641 gtggcccaca cgcggccttc tttgcggcga aagatgaata caaacgctca atgccgggcc
     2701 gtattatcgg tgtatcgaaa gatgcagctg gcaataccgc gctgcgcatg gcgatgcaga
     2761 ctcgcgagca acatattcgc cgtgagaaag cgaactccaa catttgtact tcccaggtgc
     2821 tgctggcaaa catcgccagc ctgtatgccg tttatcacgg cccgattggc ctgaaacgta
     2881 tcgctaaccg cattcaccgt ctgaccgata tcctggcggc gggcctgcaa caaaaaggtc
     2941 tgaaactgcg ccatgcgcac tatttcgaca ccttgtgtgt ggaagtggcc gacaaagcgg
     3001 gcgtactgac gcgtgccgaa gcggctgaaa tcaacctgcg tagcgatatt ctgaacgcgg
     3061 ttgggatcac ccttgatgaa acaaccacgc gtgaaaacgt aatgcagctt ttcagcgtac
     3121 tactgggcga taaccacggc ctggacatcg acacgctgga caaagacgtg gctcacgaca
     3181 gccgctctat ccagcctgcg atgctgcgcg acgacgaaat cctcacccat ccggtgttta
     3241 atcgctacca cagcgaaacc gaaatgatgc gctatatgca ctcgctggag cgtaaagatc
     3301 tggcgctgaa tcaggcgatg atcccgctgg gttcctgcac catgaaactg aacgccgccg
     3361 ccgagatgat cccaatcacc tggccggaat ttgccgaact gcacccgttt tgtccgccgg
     3421 aacaggctga aggttatcag cagatgattg cgcagctggc tgactggctg gtgaaactga
     3481 ccggttacga cgccgtttgt atgcagccga actccggcgc acagggcgaa tacgcgggcc
     3541 tgctggcgat tcgtcattat catgaaagcc gcaacgaagg gcatcgcgat atctgcctga
     3601 tcccggcttc tgcgcacgga actaaccccg cttctgcaca tatggcagga atgcaggtgg
     3661 tggttgtggc gtgtgataaa aacggcaaca tcgatctgac tgatctgcgc gcgaaagcgg
     3721 aacaggcggg cgataacctc tcctgtatca tggtgactta tccttctacc cacggcgtgt
     3781 atgaagaaac gatccgtgaa gtgtgtgaag tcgtgcatca gttcggcggt caggtttacc
     3841 ttgatggcgc gaacatgaac gcccaggttg gcatcacctc gccgggcttt attggcgcgg
     3901 acgtttcgca cctcaacctg cataaaactt tctgcattcc gcacggcggt ggtggtccgg
     3961 gtatgggacc gatcggtgtg aaagcgcatc tggcaccgtt tgtaccgggt catagcgtgg
     4021 tgcaaatcga aggcatgtta acccgtcagg gcgcagtttc tgcggcaccg ttcggtagcg
     4081 cctctatcct gccaatcagc tggatgtaca tccgcatgat gggcgcagaa gggctgaaaa
     4141 aagcaagcca ggtggcaatc ctcaacgcca actatattgc cagccgcctg caggatgcct
     4201 tcccggtgct gtataccggt cgcgacggtc gcgtggcgca cgaatgtatt ctcgatattc
     4261 gcccgctgaa agaagaaacc ggcatcagcg agctggatat tgccaagcgc ctgatcgact
     4321 acggtttcca cgcgccgacc atgtcgttcc cggtggcggg tacgctgatg gttgaaccga
     4381 ctgaatctga aagcaaagtg gaactggatc gctttatcga cgcgatgctg gctatccgcg
     4441 cagaaatcga ccaggttaaa gccggtgtct ggccgctgga agataacccg ctggtgaacg
     4501 cgccgcacat tcagaacgaa ctggtcgccg agtgggcgca tccgtacagc cgtgaggttg
     4561 cagtattccc ggcaggtgtg gcagacaaat actggccgac ggtgaaacgt ctggatgatg
     4621 tttacggcga ccgtaacctg ttctgctcct gcgtaccgat tagcgaatac cagtaattca
     4681 ctgattcgac tattttctaa aggcgcttcg gcgccttttt agtcagatga caaagtacaa
     4741 aagtgctcag acagtcccct cgcccctttg gggagagggt gaggggaaca ggccggcact
     4801 ggcgcgaata tttaccctca ccccggccct ctccctgaaa gggcgagggg gaaaagcgtg
     4861 ccaacattga agattgagcc agtttgttag caatctcaaa gatacgtcaa cgaatgaatt
     4921 tttctcggaa aaacaaatgg ctatagcact tgtgaccggt ggcagtcgcg gcatcgggcg
     4981 ggcaactgca ttactgttgg cgcaagaagg gtatacggtg gcggttaatt atcagcaaaa
     5041 cctccacgcg gcgcaggaag tgatgaactt aataacgcaa gccggtggca aagcattcgt
     5101 gctccaggcg gatatcagcg acgaaaacca ggtcgttgcg atgtttacag caatcgatca
     5161 gcacgatgaa ccgctagcag cgctggtcaa taacgccggg atcttgttta cccagtgcac
     5221 cgttgaaaac cttaccgcag agcgaatcaa ccgagtactt tccaccaacg tgacgggata
     5281 ttttctctgc tgccgcgagg cggtaaaacg catggcgctt aaaaatggtg gcagtggcgg
     5341 cgctatcgtc aatgtctctt cggtggcctc acggttgggt tcgccagggg aatatgttga
     5401 ttacgcggca tcgaaagggg cgattgatac gttaaccacc ggactatcgc tggaagtcgc
     5461 cgcgcagggg atccgcgtta actgcgtgcg gccagggttt atttataccg aaatgcacgc
     5521 cagcggcggc gagcctggac gcgtcgatcg cgttaagtcg aacatcccca tgcagcgtgg
     5581 tggacaggca gaagaggtcg cgcaggccat tgtctggcta ctaagtgata aagcctctta
     5641 cgtcacggga agttttatcg atttggcggg cgggaaataa aacagggaag ttgtctgacc
     5701 ggatgcaaca agtattgcat ccggtacttc atcgacttaa agcttctcgc cgttgctggc
     5761 aatcacctct tggtaccagt taaagctctt cttacgtgaa cgcgacatat cgccagcacc
     5821 gtcgtcatgt ttattcacat agataaagcc gtagcgtttg ctgtactgcc cggtggtgaa
     5881 cgatacgcag tcgatgcagc cccacggtgt gtagcccatc agatccacgc catcgtaagt
     5941 caccgctttt ttcatctctt cgatatgggc gcgcaggtag tcaatgcggt agtcgtcgtt
     6001 gatgctgcca tcttcttcca ctttgtcgta agcgccaaaa ccgttttcga caataaacag
     6061 cggcctctga taacgctcat acagttcgca aagggcatag cgcagaccta ctggatcaat
     6121 ctgccagccc cagtcagacg ctttaacata cgggttcggt acgctgcctt cgaaaccaga
     6181 gatcgcatcg ccggtgccgc cttcggcctt cactgcgttg gtcatgtaat agctgaaacc
     6241 aagataatcg caggtgcctt cacgcagtac atcgagatcg ccgtcttcca ttttgatgtt
     6301 aaatccgcga cgctcccact cgttcaacac ataggacggg taatagccgc gtagctgcac
     6361 atcggtaaag acgtagcgtt cgcgcatcga ctcctgagcg aacatcacat cgtccgggtt
     6421 acagcagtag ggatagagcg gcaccatcgc cagcatacag ccgactttca tctccgggtg
     6481 aatgcgacgc gcagctttca ccgccagggc gctggcgaca aactggtgat gcagcacctg
     6541 atacatcgtc tcttccgggt tctcatgctc ggtataaacc acgccggagc agcagtaacc
     6601 gaacagcggt gcacgccagt tacgctggtt gttaatttcg ttgaaggtca tccagtattt
     6661 gactttatgc ttatagcgtt caaacaccac ttccgcgaaa cggacaaaga aatcaaccac
     6721 tttacggttg gtccagctac cgtattgctg caccagatgc agcggcattt caaagtggga
     6781 gagggtgatc accggttcga tgttgtattt cagcagttca tcgaacatat catcgtagaa
     6841 cttcagccct tcttcgtttg gctgagcttc atcacctttt ggaaaaatac gcgtccaggc
     6901 gatggaagtc cgaaaacatt tgaagcccat ttcggcaaat agcttgatgt cttccttgta
     6961 gtgaccataa aaatcaacgg cttcatggtt tggatagtat ttgcctggca agacttcttt
     7021 ggtgatttcg cgcggcacgc cgtgtgcgcc accggtcaga acgtcacaaa tgctcggccc
     7081 ttttccgcct ttgttccagc cgccttcgac ctgatgagcg gcaactgcgc cgccccataa
     7141 gaaatctttc ggtaaggtga gttttttcac tatcataggc tccagatgag tcaattctta
     7201 caggaaatag taccatgcag ccaaacgaca tcactttttt tcaacgtttc caggatgaca
     7261 ttctggctgg gcgtaaaacc atcaccatcc gcgacgagtc tgaatcgcac ttcaaaacgg
     7321 gtgatgtgct tcgtgtcgga cgttttgaag atgacggtta tttttgcacg attgaagtca
     7381 ccgcaacctc aaccgtaacg ctggatacgc tgactgaaaa acatgcagag caggaaaata
     7441 tgaccctgac tgaactgata aaggtcattg ccgacatcta tcccggtcag acacagtttt
     7501 atgtgattga atttaaatgt ctttaaattg aactcgcgcc tgttagttga aatttcgcat
     7561 ttaatttcat gatttttagg agattagtct caaatcagaa tttattttag ctaacaggtg
     7621 ttcactggaa ctattctcag ttacgctgga gaggtataca tgtgcgtgta cgtttctccg
     7681 gagtaagtta tggttcagaa gcccctcatt aagcagggat attcgctggc agaggaaatt
     7741 gccaacagcg tcagtcacgg cattgggttg gtgtttggta tcgttgggct ggtgttgcta
     7801 ctggttcagg cggtggatct caatgccagc gccactgcga taaccagtta cagcctctat
     7861 ggcggcagta tgatcctgct gttcctcgct tcgacgctct atcacgccat tcctcatcaa
     7921 cgggcaaaaa tgtggctgaa gaaatttgac cattgcgcta tttacctgtt gattgccgga
     7981 acctacacgc cgtttttgct ggtggggctg gattctccgt tagcgcgcgg gttgatgatt
     8041 gttatctgga gcctggcatt gctgggtatt ctgtttaaac tgaccatcgc gcaccgattc
     8101 aaaattttat ctctggtgac ctatctggcg atgggctggc tgtcgctggt ggtaatttat
     8161 gaaatggcag ttaagctcgc ggcgggcagc gttaccttac tggcggtagg cggcgtggtt
     8221 tattcgctcg gggtgatttt ctacgtctgc aaacgcattc catacaacca tgccatctgg
     8281 cacggcttcg tgctcggcgg tagtgtgtgc cactttctgg cgatctattt gtatattggg
     8341 caggcgtaat gggtatgcct ttgtaggcct gataagacgc gccagcgtcg catcaggcat
     8401 cggagcacaa ctgccggatt cggcgtgaac gccttatccg atctacgggc ctcgtccctg
     8461 ttgtgataaa ggcatcaaat cccagacatg ataagacgcg tcagcgtcgc atcaggcgat
     8521 aaatgcctga tgcgataaag agatttactc ttcgagcgaa tacggcagcg gctcgatatg
     8581 caatgtattc gcatcgtcgc gtacgcggaa gatgctatcc ggttccatat cgttattcat
     8641 gacgacctgt accacgacct gaccatcttc cagttttacc gcagccagca ccgtaccggt
     8701 acgacgccag ttctcgccca ttttcagctc taagtcttca ccagcttccg gcagtcggct
     8761 ggcgctacct gccagcaacc agagcgcacg tttattggca ccacggaatt ttgctcgcgc
     8821 caccatctct tgtccggtat aacagccttt cttaaagctg ataccgccca gcgcctggag
     8881 attggtcgcc tgtgggataa actgcccgct gttggcggca tcaatcaccg ggaaacccgc
     8941 ttcaatgttt aatgccagcc actgttggct attgttcagt tccgcttcac cgcgcagttt
     9001 atcggtcagc atattagcag tagcttcatc ggttacgatc aggaaacgtt ctgccgggtg
     9061 ttcaaaccat agcaaagtgg tcgcgccttc tttgactacc tgtttttctt tcgaaggcag
     9121 ttcgctaaag agatttgcca gcgcggcgcg cgcctgaaaa ccggcaacac caagcagcac
     9181 acgctcgtcg tctggcgcga tggtcacttt agagaatacc gcatattttt tcagttcagt
     9241 cagctgcggt tcacgcacgc tgcgccgttc aatccatgca aagccatcgc cgtcgcggaa
     9301 cagacgtaaa ttgctccaca ttttaccttt ggcgtcgcaa tgggcggcga gcaggtgctg
     9361 attttctgtc atctggctga catctgctgt cacctgaccc tgcatatatt tttcgctgtc
     9421 cgcgccagta atggtggcaa gcgcccagtc atcaagcgtc atcagcgtca gcggtaaacg
     9481 ggcagaagcc gtaggctgac ggggaggaaa aggtgtaaaa gccataatag agtcctgagt
     9541 tgcttaacgg cgtattaatg actaatggta aaagagctat gaggcaatgc aagtgctttc
     9601 actacgcgat ctgtgccttc tgcgaggcga cttccaaaaa aatgaagcca ttgaattgtt
     9661 gtgaattaat ttcgcgatcg cgatggtgtt tactgatatt cccgtaaaag aaggcgaaaa
     9721 acacgttaca atagcggggt actggatttt cgtgagacag gaagaagatg gacattaaca
     9781 acaaagcccg cattcattgg gcatgccgcc gtggtatgcg cgaactcgat atttcaatca
     9841 tgccgttttt cgaacatgag tacgacagct taagcgatga cgaaaaacgc atctttattc
     9901 gtctgctgga atgtgacgat ctggacctgt ttaactggct gatgaatcac ggtaaaccgg
     9961 ccgatgcaga actggaaatg atggtccgac tcatccagac acggaaccgg gaacgtggtc
    10021 ctgtggcaat ctgatttgcg cgtctcctgg cgcgcacagt ggctttcctt gctgattcat
    10081 gggctggttg ccgctgttat tttactcatg ccctggccac tcagttacac cccgttatgg
    10141 atggtgttac tttcgctggt ggtgtttgat tgcgttcgca gccagcggcg tattaatgct
    10201 cgccaggggg aaattcgctt gttgatggac gggcgtttgc gttggcaagg gcaggagtgg
    10261 agcatcgtca aagcaccgtg gatgattaag agcggcatga tgctgcgttt acgttctgat
    10321 ggcggtaaac ggcaacattt atggctggca gccgacagca tggacgaagc tgaatggcgg
    10381 gatttacggc ggattttgtt gcaacaagag acgcaaagat aaagatgacg gcaaagcata
    10441 tgcgctaagc cgtcagtgag atcaggcgta atgctctgcc atttcgttga ggatttgctc
    10501 gcaccagctc tgaatacgct cgtcgctaag gtcatactgg ttagtttcat ccagcgccag
    10561 acccacgaac agttgcccgt cagcaatcac cggtttcggg ctggtaaatt catatccttc
    10621 cgttggccag tagccgacga acttcacgcc tttggtcgag agtttgtcat gcagcatacc
    10681 gagcgcatcg aggaaccact cgccgtatcc cagttgatcg ccaagcccat acagcgcaac
    10741 aattttacct tcaaggttca ggtcgtcgag ctgatcccag acggcttccc agtcttcctg
    10801 gatttcacca aaatcccagg tcgggatacc cagaatgagc acatcgtact gctccattaa
    10861 tttcggggag tcgtccttga ggttatgtaa ggtcaccagt tctgggccga taatatcgcg
    10921 gattttttct gccgccattt cggtgtaaca ggtgctggaa ccgtaaaaaa gacccatatt
    10981 cataacgtaa gcttctcgat tctgctgtgg cttttgcagc tagtgtatca gaatcgcttt
    11041 agtcacggca caataaggca taatggatca aatgagtgac cataaggggc gcaagtgaaa
    11101 caggatctgg cacgcatcga gcagtttctt gatgctctgt ggctggaaaa aaatctggct
    11161 gaaaatacgt tgaacgctta ccgtcgcgat ctgtcaatga tggtggagtg gttgcatcac
    11221 cgcgggttga cgctggcgac ggcgcaaagt gacgatttgc aggcattact ggcagaacgg
    11281 ctggagggcg ggtataaagc caccagctca gcgcgtttgc tgagtgcagt gcgccgattg
    11341 ttccagtatc tttatcgcga aaagtttcgt gaagacgatc ccagtgcgca tctcgcttca
    11401 ccgaaattgc cccagcgttt gccaaaagat ttaagcgaag cgcaggtcga acgtttatta
    11461 caggcaccat taattgatca gccactggag ctacgcgata aagccatgct tgaagtgttg
    11521 tatgctaccg ggctgcgtgt ctctgaactg gtcggactga caatgagtga tatcagcctg
    11581 cgtcagggcg tagtgcgggt cattggtaaa ggcaacaaag agcgcctggt accgttaggt
    11641 gaagaggcgg tttactggct ggaaacctat ctggaacatg ggcgtccgtg gctgctgaat
    11701 ggtgtgtcaa ttgacgtgtt gtttcccagc cagcgtgcgc agcagatgac gcgacagacc
    11761 ttctggcacc gcattaaaca ttatgctgtg ctggcgggta tcgacagcga aaagctgtca
    11821 ccgcatgtgt tgcgtcacgc ttttgccact catttattaa atcatggtgc ggatttacgc
    11881 gtggtgcaga tgctactggg ccacagcgat ctttccacca cgcaaattta tacgcatgtc
    11941 gctaccgagc gtctgcggca acttcatcaa cagcatcacc cgcgggcgtg atgtctgaaa
    12001 agaacgggaa gatttatgaa gaaaggtttt atgttgttta ctttgttagc ggcgttttca
    12061 ggttttgctc aggctgatga cgcggcaatt caacaaacgt tagccaaaat gggcatcaaa
    12121 agcagcgata ttcagcccgc gcccgtagct ggcatgaaga cagttctgac taacagcggc
    12181 gtgttgtaca tcaccgatga tggtaaacat atcattcagg ggccaatgta tgacgttagc
    12241 ggcacggctc cggtcaatgt caccaataag atgctgttaa agcagttgaa tgcgcttgaa
    12301 aaagagatga tcgtttataa agcgccgcag gaaaaacacg tcatcaccgt gtttactgat
    12361 attacctgtg gttactgcca caaactgcat gagcaaatgg cagactataa cgcgctgggg
    12421 atcaccgtgc gttatcttgc tttcccgcgc caggggctgg acagcgatgc agagaaagaa
    12481 atgaaagcta tctggtgtgc gaaagataaa aacaaagcgt ttgatgatgt gatggcaggt
    12541 aaaagcgtcg caccagccag ctgcgacgtg gatattgccg accattacgc acttggcgtc
    12601 cagcttggcg ttagcggtac tccggcagtt gtgctgagca atggcacact tgttccgggt
    12661 taccagccgc cgaaagagat gaaagaattc ctcgacgaac accaaaaaat gaccagcggt
    12721 aaataattcg cgtgaaacaa cagatacaac ttcgtcgccg tgaagtcgat gaaacggcag
    12781 acttgcccgc tgaattgcct cccttgctgc gccgtttata cgccagccgg ggcgtgcgca
    12841 gtgcgcaaga actggaacgc agtgttaaag gtatgttgcc ctggcagcaa ctgagcggcg
    12901 tcgaaaaggc cgttgagatc ctttacaacg cttttcgcga aggaacgcgg attattgtgg
    12961 tcggtgattt cgacgccgac ggcgcgacca gcacggctct aagcgtgctg gcgatgcgct
    13021 cgcttggttg cagcaatatc gactacctgg taccaaaccg tttcgaagac ggttacggct
    13081 taagcccgga agtggtcgat caggcccatg cccgtggcgc gcagttaatt gtcacggtgg
    13141 ataacggtat ttcctcccat gcgggggttg tacacgctcg ctcgttgggc attccggtta
    13201 ttgttaccga tcaccatttg ccaggcgaaa cattacccgc ggcggaagcg atcattaacc
    13261 ctaacttgcg cgactgtaat ttcccgtcga aatcactggc aggcgtgggt gtggcgtttt
    13321 atctgatgct ggcgctgcgc acctttttgc gcgatcaggg ctggtttgat gagcgtggca
    13381 tcgcaattcc taacctggca gaactgctgg atctggtcgc gctgggaaca gtggcggacg
    13441 tcgtgccgct ggacgctaat aatcgcattc tgacctggca ggggatgagt cgcatccgtg
    13501 ccggaaagtg ccgtccgggg attaaagcgc tgctggaagt ggcaaatcgc gatgcgcaaa
    13561 aactcgccgc cagcgattta ggttttgcgc tggggccacg tctcaatgct gccgggcgac
    13621 tggacgatat gtccgtcggt gtggcgctgt tgctgtgcga caacatcggc gaagcgcgcg
    13681 tgctggcaaa tgaactcgat gcgctaaacc agacgcgaaa agagatcgaa caaggaatgc
    13741 aagttgaagc cctgaccctg tgcgagaaac tggagcgaag tcgcgacacg ctacccggcg
    13801 ggctggcaat gtatcacccc gaatggcatc agggcgttgt cggtattctg gcttcgcgca
    13861 tcaaagagcg ttttcaccgt ccggttatcg cctttgcgcc agcaggtgat ggtacgctga
    13921 aaggttcagg tcgctccatt caggggctgc atatgcgtga tgcactggag cgattagaca
    13981 cactctaccc tggcatgata ctgaagtttg gcggtcatgc gatggcggcg ggtttgtcgc
    14041 tggaagagga taaattcgaa ctctttcaac aacggtttgg cgaactggtt acagagtggc
    14101 tggacccttc gctattgcaa ggcgaagtgg tgtcagacgg tccgttaagc ccggccgaaa
    14161 tgaccatgga agtggcgcag ttgctgcgcg atgctggccc gtgggggcag atgttcccgg
    14221 agccgctgtt tgacggtcat ttccgtctgc tgcaacagcg gctggtgggc gaacgtcatt
    14281 tgaaggtgat ggtcgaaccg gtcggcggcg gtccactgct ggatggtatt gcttttaatg
    14341 tcgataccgc cctctggccg gataacggcg tgcgtgaagt gcaactggct tataagctcg
    14401 atatcaacga gtttcgcggc aaccgcagcc tgcaaattat catcgacaat atctggccaa
    14461 tttagcgtca tcttctctat aaaaaagagc gtggattggg tacaatcccg ctcttatcac
    14521 cgcattttga ctagctcaat aaaagaaatc agaccatgtt tgaaattaat ccggtaaata
    14581 atcgcattca ggacctcacg gaacgctccg acgttcttag ggggtatctt tgactacgac
    14641 gccaagaaag agcgtctgga agaagtaaac gccgagctgg aacagccgga tgtctggaac
    14701 gaacccgaac gcgcacaggc gctgggtaaa gagcgttcct ccctcgaagc cgttgtcgac
    14761 accctcgacc aaatgaaaca ggggctggaa gatgtttctg gtctgctgga actggctgta
    14821 gaagctgacg acgaagaaac ctttaacgaa gccgttgctg aactcgacgc cctggaagaa
    14881 aaactggcgc agcttgagtt ccgccgtatg ttctctggcg aatatgacag cgccgactgc
    14941 tacctcgata ttcaggcggg gtctggcggt acggaagcac aggactgggc gagcatgctt
    15001 gagcgtatgt atctgcgctg ggctgaatcg cgtggtttca aaactgaaat catcgaagag
    15061 tcggaaggtg aagtggcggg tattaaatcc gtgacgatca aaatctccgg cgattacgct
    15121 tacggctggc tgcgtacaga aactggcgtt caccgcctgg tgcgtaaaag cccgtttgac
    15181 tccggcggtc gtcgccacac atcgttcagc tctgcgtttg tttacccgga agttgatgat
    15241 gatattgata tcgaaatcaa cccggcggat ctgcgcattg acgtttatcg cgcgtccggc
    15301 gcgggcggtc agcacgttaa ccgtaccgaa tctgcggtgc gtattaccca catcccaacc
    15361 gggatcgtga cccaatgcca gaacgaccgt tcccagcaca agaacaaaga ccaggccatg
    15421 aagcagatga aagcgaagct ttatgaactg gagatgcaga agaaaaatgc cgagaaacag
    15481 gcgatggaag ataacaaatc cgacatcggc tggggcagcc agattcgttc ttatgtcctt
    15541 gatgactccc gcattaaaga tctgcgtacc ggggtagaaa cccgcaacac gcaggccgtg
    15601 ctggacggca gcctggatca atttatcgaa gcaagtttga aagcagggtt atgaggaacc
    15661 aacatgtctg aacaacacgc acagggcgct gacgcggtag tcgatcttaa caatgaactg
    15721 aaaacgcgtc gtgagaagct ggcgaacctg cgcgagcagg gggttgcctt cccgaacgat
    15781 ttccgtcgcg atcatacctc tgaccaattg cacgcagaat tcgacggtaa agagaacgaa
    15841 gaactggaag cgctgaacat cgaagtcgcc gttgctggcc gcatgatgac ccgccgtatt
    15901 atgggtaaag cgtctttcgt taccctgcag gatgtgggtg gccgcattca gctgtacgtt
    15961 gcccgtgacg atctgccgga aggcgtttac aacgagcagt tcaaaaaatg ggacctcggc
    16021 gacatcctcg gcgcgaaagg taaactgttc aaaaccaaaa ccggcgaact gtctatccac
    16081 tgcaccgagc tgcgtctgct gaccaaagca ctgcgtccgc tgccggataa attccacggc
    16141 ttgcaggatc aggaagcgcg ctatcgtcag cgttatttgg atctcatctc taacgatgaa
    16201 tcccgtaaca cctttaaagt acgctcgcag atcctctctg gtattcgcca gttcatggtg
    16261 aaccgcggct ttatggaagt tgaaacgccg atgatgcagg tgatcccagg cggtgccgct
    16321 gcgcgtccgt ttatcaccca ccataacgcg ctggatctcg acatgtacct gcgtatcgcg
    16381 ccggaactgt acctcaagcg tctggtggtc ggtggcttcg agcgtgtatt cgaaatcaac
    16441 cgtaacttcc gtaacgaagg tatttccgta cgtcataacc cagagttcac catgatggaa
    16501 ctctacatgg cttacgcaga ttacaaagat ctgatcgagc tgaccgaatc gctgttccgt
    16561 actctggcac aggatattct cggtaagacg gaagtgacct acggcgacgt gacgctggac
    16621 ttcggtaaac cgttcgaaaa actgaccatg cgtgaagcga tcaagaaata tcgcccggaa
    16681 accgacatgg cggatctgga taacttcgac tctgcgaaag cgattgctga atctatcggt
    16741 atccacgttg agaagagctg gggtctgggc cgtatcgtta ccgagatctt cgaagaagtg
    16801 gcagaagcac atctgattca gccgaccttc attactgaat atccggcaga agtttctccg
    16861 ctggcgcgtc gtaacgacat taacccggaa atcacagacc gctttgagtt cttcatcggt
    16921 ggtcgtgaaa tcggtaacgg ctttagcgag ctgaatgacg cggaagatca ggcgcaacgc
    16981 ttcctggatc aggttgccgc gaaagacgca ggtgacgacg aagcgatgtt ctacgatgaa
    17041 gattacgtta ccgcactgga acatggctta ccgccgacag caggtctggg gattggcatc
    17101 gaccgtatgg taatgctgtt caccaacagc cataccatcc gcgacgttat tctgttcccg
    17161 gcgatgcgtc cggtaaaata agcattacgt tatgctcaca accccggcaa atgtcggggt
    17221 tttttaattg tgctgcgcga aagcagacaa caattttctt gcttcactat tggctgcctg
    17281 catcaccatc cacggactga acgcccacgg tgtggcatca ataccgtgta atacatctgc
    17341 taaatcacac cattgataat ccatcacttc atcatcgttg atctgtaacg cactggtggt
    17401 gcgtgcggca aataccggac acacttcatt ttccacaatg ccattcggat cggtggcgcg
    17461 atagcgaaag tcaggataga tagattcagg aggcgtaatt tccacgccaa gcccataacg
    17521 gcaacggcgg atcaccgcgt cttcgttgct ttctcccagt tgtgggtgcc cacaaaccga
    17581 gttagtccac acgccaggcc acgctttttt gctcagtgcg cggcgggtaa ctaataattg
    17641 tcctttggca ttaaacagcc aactggagaa cgcgcgatgt aagcgggtgt ctgccgtgtg
    17701 tgcggcatac ttttccagcg tacccgtggg aactccctgt gcattcaata aaatgacgtg
    17761 ttccgtttgc ataatttctc acatgtaatt ctgatcactc atcattatag ataattgaag
    17821 agtgaaatga ttaaccaagt gagcgtttat cgacaaccgc ccgttttgag cggatgccga
    17881 caggtaaaaa ccatttaaaa aagtagcatc atatatttga agcgactttc aatggccatt
    17941 gcaacacagt cagaaatgac aggaatagag agagtaaaaa gcaacaacag actgaatata
    18001 gcgcgcatta aaaaattcat attattctcc atgctcattt ttcttcagct tgcgcatcag
    18061 aagccacata cgtactgtcc tgacttttaa atctttatca gagacggatt ccttcaccag
    18121 gtcagcatga tgctgatagc cattaaagaa gacattcagc acaacggcgc taagggttgc
    18181 gagcataatg ccgctatgca gcaacggttg cagtacggcc ggtaacttag aaaagaaatc
    18241 gtgagagagc gtcggagtca tgccaacgcc gagactgatc gccacaatat agaggttgta
    18301 acggttggtg gtgtagttac agcgcgacag aattcgaatc cctgtcgcca gtaccatgcc
    18361 gaacatcacc agcccagcgc cgcccagcac aaattgcgga atggaggcta ccagcaccgc
    18421 catttttggc accatgccaa acaggattaa tataattccc gaagaaatac acacccagcg
    18481 gctatgaacg cgcgtcacgc taaccaggcc aacgttttga gaaaatgacg tgtgggggaa
    18541 gctgttaaac gtgccgccta tcattgtccc tacgccatcg acacgcagcc cgcgaataat
    18601 atcgtgtgaa gagagtttac gaccgactat ttcacccagt gccaggaaca tccccatcga
    18661 ctcgataaac acgatgatta acacggcagt catggtcaga atggaaacgg gatcgaaaat
    18721 cggcatacca aacgacatcg gggtgacaat cgcaaaccac gaggcatcat gtagcccgga
    18781 taaattgact tcattcatca tccacgaaag taaaaagcca aatacaatcc ccagtaatac
    18841 ggcgacgttg gacataaatc ctttcgcata gcgagtaatg agcaagataa aaattaagac
    18901 ggcaaaggag atacctaaat aaacgggatt accatattgc ggattccctt tacctccggc
    18961 ggcccagtca atacccacct gaatgatgct aagcccaata gaagtaataa ccacaccggt
    19021 aaccagtggc gggaataaag gcatcaagcg accaataagt ggcgctaata atgtggtgat
    19081 aaaacccgcg gctatagtgg caccaaatat ccccagcagg ccgatatccg ggttcatccc
    19141 aatggctatc attggtgtta cagcagcaaa ggtcaccgac ataatcaccg gcaggcggat
    19201 ccccataaag cggccgatac cgatacattg caataatgtg acgatcccgc agcaaaagag
    19261 atccgagcta atgagcatcg caatagcttc ttttgagagg cccagtcggt caccaatcat
    19321 aagaggaaca gcgactgcac ctgcgtacat gacaaggacg tgttgcagac cgagtatgat
    19381 cagctttcct ggtgataata tgcggtcaac ctcatcagtt gggcggtctt gcccagaaga
    19441 tgagggaagt tgggaatcta tggcgctcat cagtgtttct cctttcgccc gccagagtaa
    19501 aaagcaaccg tttacgttaa tgaaactgtc taaacacatg ctcgtggagt gccagttttt
    19561 gcgaggaagg ggagttagag acaggaattg cggaggcaat cacataatcg tggttaaacc
    19621 acgaaaatga gaagagaacc tgacaatgaa attatctgta attttctcat attgatattt
    19681 cattaatcat tctggcatgt cgcactctcg catttaatcg tttttatctg gatagcgctc
    19741 ttttggtcgg caatgttgaa ataatgaaag cggcatagat tgaggggcaa taatttgaac
    19801 tcagtcgcaa aattataatt aaaacggtca tgctacctaa tatatgcccg tttattgcat
    19861 cgcgaaattt gagaagacat aactcattaa atgaaaggga tgcaaatgaa taagtttatc
    19921 gctgctgaag ctgcggaatg tataggctgt catgcttgtg aaattgcctg tgcggtggca
    19981 cacaatcaag aaaactggcc gctgagtcac agtgactttc gaccgcgtat ccacgttgta
    20041 gggaaaggcc aggctgcgaa tccggtggcc tgccatcact gcaacaatgc cccttgcgtt
    20101 acggcttgtc cggttaatgc tctgactttc cagtccgata gcgtacaact ggacgagcaa
    20161 aaatgtattg gctgtaaaag atgcgcaatc gcttgcccct ttggcgtcgt tgagatggtc
    20221 gatacgattg cgcagaaatg cgacctttgt aaccagcgca gttccggcac gcaagcctgt
    20281 attgaagtct gcccaacaca ggcgttacga ctgatggacg ataaagggtt acagtggata
    20341 aaggtggctc gccagcgcaa aacggcagca ggaaaagcgt catcagatgc tcagccatct
    20401 cgcagtgcag cgttgctccc cgttaactcg cgtaaaggcg cagataaaat ttcagcgagt
    20461 gaacggaaaa cccactttgg cgaaatatat tgcgggctgg atccactgca agcgacttat
    20521 gagagtgacc gctgtgttta ttgtgccgaa aaagctaact gcaactggca ttgcccgctg
    20581 cataacgcta ttccggatta catccgtctg gtacaggaag gaaagattat tgaagcggca
    20641 gaactttgcc accagaccag ttccttacca gaaatttgcg gcagggtatg tccacaggac
    20701 cgtctttgtg aaggcgcatg tactttgaaa gatcactcgg gcgcagtctc tatcggtaat
    20761 ctggaacgct acatcaccga taccgcgctg gcgatgggct ggcgtcccga tgtcagcaaa
    20821 gttgttcccc gtagcgaaaa agtggcggtg attggcgctg ggccagcagg gttagggtgt
    20881 gctgatattc tggcgcgcgc gggagttcag gttgatgtct ttgatcgcca tccagaaatt
    20941 ggcggtatgc tgacttttgg cattcctcct ttcaaactcg ataaaacggt attaagccag
    21001 cggcgagaga tattcaccgc aatgggaatc gactttcatc ttaactgtga aattggccgc
    21061 gatattacct ttagcgattt aacttctgaa tatgatgcag ttttcatcgg cgtggggact
    21121 tacgggatga tgcgagcaga tctgccgcat gaagatgcgc ccggtgtcat tcaggctcta
    21181 ccgttcctga ctgcccatac ccgccagctc atgggattgc cggagtctga agagtatccg
    21241 ctgacggacg tggaaggtaa gcgagtcgtg gtattgggcg gtggcgatac gacaatggat
    21301 tgtttgcgga cttccatccg cctcaatgcc gccagcgtga cctgcgcgta tcgtcgtgat
    21361 gaagtcagta tgccgggctc gcgcaaagag gtggttaatg cgcgcgagga aggtgtcgag
    21421 tttcagttca atgttcaacc gcaatatatc gcttgtgacg aagatgggcg tttaactgcg
    21481 gtgggcctga ttcgtaccgc catgggtgag ccggggccgg atggtcgccg tcgtcctcgt
    21541 ccggtagcgg gttcagagtt tgaattgccc gccgatgttc tgattatggc ctttggtttc
    21601 caggcgcatg ccatgccgtg gttgcagggc agcggaatta aactcgataa atggggcctg
    21661 attcaaaccg gtgacgtcgg gtatttacct acccagacgc atctgaaaaa agtctttgct
    21721 ggtggtgatg cagttcatgg cgcggatctg gttgtcactg caatggccgc aggaaggcag
    21781 gcggcgcgcg atatgttaac tctgtttgat acgaaggcat cgtgatgaaa tcgttaatta
    21841 tcgttaatcc ggctgactgt attggctgcc gtacttgtga agtagcctgt gtggtcgctc
    21901 acccttcaga acaggagttg aatgccgatg tctttttacc ccggctgaag gtacagcgac
    21961 tggacagcat cagtgcgccg gtgatgtgcc atcagtgtga aaacgcccct tgtgttggcg
    22021 cttgccccgt gggggcgctg acgatgggcg agcaggtggt gcagaccaat tctgcccgtt
    22081 gtattggctg tcagagttgc gtcagtgcgt gcccgtttgg aatgatcacc attcagtcgt
    22141 tgccggggga tacccgacaa caaattgtga aatgcgatct ctgtgaacag cgggaagagg
    22201 ggccagcctg tgttgaatct tgcccgacac aggcgctgca gttgcttact gaaagagaac
    22261 tcaggcgagt ccgccagcag cgtattgttg ccagcggtga gaatcctctg tgaaagaaat
    22321 ctccgcgctg aaaggcgcgg agaatctatt agattgccca accacccgcg taaaatgcga
    22381 ccagtgcggc agtaatgatc accgtaccaa tattcaactt ttgccattcg cgagcaaaga
    22441 cacggcctac gaccagtgtc acaaagccca gcataatacc ggtaacgata ttacaagtca
    22501 gaacgatgaa cacggcacac accaggccag ccattgcgtc aataaaatcg ttgaaatcca
    22561 gcttcgagac gttacttaac atcagcaaac ccacgtacat cagagcgggt gcagtggcgt
    22621 aaccagggat caaaaatgac agcggtgata agaacagaat cagcaggaat aacgccccca
    22681 ctacggttgc ggtcaaccct gttttacccc cggcggcggt tcctgccgct gattcgatat
    22741 aaaccgctgc gggcgctgcg cccaccagac cggagaatat tgaacttact gagtcactgg
    22801 tcagggcttt gccgccattg atgatctggt tgtctttatc caacaaattc gcctgaccgg
    22861 cgacggcacg gatggtgcca gtggcgtcga acactgcggt catcaccaat gccagtacac
    22921 tcggaagtac ggttggctgg agtgcgccca taatatcgag gctgaaaatc agagacttac
    22981 catcttcgcc agtcagactt ggcatcgcca ccagaccgtg gtatttcacc gctgggtcaa
    23041 agattaagcc gataatcgaa attgcgataa tcaccaacaa gatcccgccg ggtacgcggc
    23101 acttctccag accgaagatg accgccagcc ccagcaagct catcatcacc gggaaggagg
    23161 taaacgcacc gagcgccact ggcaagcctt caatcgggtt tttgataacc atacccacac
    23221 cgttagcagc aatcagcagc agaaacaggc cgataccgat acctgtaccg tgagcgatac
    23281 ccatcggtaa attacgtaag atccaggtac gcacaccggt tacggaaata gcggtgaaga
    23341 tgacgcccat cagaaagacc gcgcccagtg cgacaggaac gctaatttgt tgcccgagta
    23401 ccagactgaa tgcggtaaat gccgtcaagg aaatcgcaca accaatcgcc attggcaaat
    23461 tggcccacaa ccccatcagc aacgagccga agcccgcgac cagacaggtg gcaacaaaca
    23521 cagctgcggg aggaaaacct gctttgccca gcattcccgg aacgacgata acggaataaa
    23581 ccatggccag aaaggtcgtt aagccagcca gtacttcctg acgaacggta ctgccacgag
    23641 cggtaatttt aaaataatta tccagcgcgc cctgtggctt tggtgcgtcc ggtgtttgta
    23701 ggatgtctcc agacataatg atgtcctcac gaggatgtta aagttttatt attagttgcg
    23761 ttcgtacacc agacgaccat caacgtaggt gcggtagatc gaacggtcat cgcccaacgt
    23821 catcatcacg aacaatttgt cgactaaaga aacagagttg tcatagcgca gctgctgtag
    23881 cggagtggcg gtgggttcca tcaccacgaa atcagcctct ttgccaggta aaaagttgcc
    23941 aatcaaatcg tcaaggccca gagatttcgc tccgccgagc gtggccaggt aaaacgcttc
    24001 atatgccgag aggcgatagc cttgtaattg caatactttg taggcttcgt tcagcgtttg
    24061 cagcatgttg aaagtggttc cggcaccgat atccgttccc atgcccactt taactttctt
    24121 ctgccatgct tttttcaagt tgaataagcc gctgccgagg taaaggttgg aggtcggaca
    24181 gaaagcaatg ctggatttgg tttcgctgag acgatcccac tctttttctt cgagatggac
    24241 gcagtgagca aagacacagt ttttaccggt caggccgtac tgatggtaaa catccagata
    24301 accatcatgg tcaggataaa gcgatttcac ccaggcaatt tcatctttgt tttcacagag
    24361 atgggtatgt acccacgtat ccggatattc ttctttcagg cgttgcgcca tcgccatctg
    24421 ttcaggagat gaggtcgggg cgaagcgtgg cgtaatcgca tatagcagac gaccattttt
    24481 gtgccagcgt tcaatcagtt ctttgctttg gtgatagctg ctttcggcag tgtcgagcag
    24541 ataatccggt gcgttgcggt ccatcatcac cttaccggca atcatacgca tattgatatg
    24601 actggcggct tcaaacagcg catcaacaga ttgcggatga acagtgccaa acaccagcgc
    24661 cgtggtggtt ccgttacgta aaagctgctt gatgaagaat gccgacattt cgcgggcgta
    24721 ctctaaatcc tcataacgac gttcagtagg gaaggtgtgt ttattcaacc actccagcaa
    24781 ttgttcacca taggccccca ccatttcact ttgcggataa tggatatgtg tatcgacaaa
    24841 gcccggtact atcagtttgc cgcgatagtc gcgcacgcga atagtgtcag gaatttgatg
    24901 ctttccgtct tcccattcgc caaaccattc cacttttccc tgtttaatga gtaataaacc
    24961 atcctcaata aaccgcagcg cagaggcaat ctcttccggg ttatcgaccg tacgggtgac
    25021 atcaataaaa ctgcctcgta ccgctttcaa cgtgtgttct cctgacatca ttgactcctt
    25081 tactgttaat ccatctcttc cgctgaggta ataccaggca gaacgttttc ctgtcggtag
    25141 ccaccaggga ggataatatt taataaaatc gcagttaacc cgccagcaca aatagggttt
    25201 tcaactagta cataaataga ggctggcaat attttaaaaa tttcgggatc gtaggagacg
    25261 ccaagtccta aacctaaaga agtggcgaca ataagtgttt ctcggcgctt taaaccgttg
    25321 gtgatgatta tgcgaatccc agcgatggca atcatggaaa acatcaacgt cattgcgcct
    25381 cccagaactg ccgagggaat ggtcgtgaag aagccgccaa tcatcggaaa caaaccgagg
    25441 ataaccagca ttaccgcgat ggttcgcccg acataacgtg aagcgacgcc agtcatctga
    25501 ataaccccat tattttgcgc aaacgtggtt aagggtaatg acccgacagc ggaggcgata
    25561 acagaaacca gaccatctgc cagcacgccg cctttcagtc gggactgata ctcttccccc
    25621 tgaatggggc ggcgggaaac cattgccgtg gcggtgatat cgccgactgc ttccagcacg
    25681 ctaagcagat aaatcgtgcc aaccaccagg aactgatgga agctaaaact aaagccgtat
    25741 ttgaacggat gcgggatggt gattaacggc aaattgcgca tactgctgaa atccaccatg
    25801 cccaggcata acgatgcgat atagccgaca catagcccaa tggcgatccc tcccatgcgt
    25861 agcaacggac tgcgacagca gttaaagccg atcaccacaa ttaaaaccaa taaaccaacg
    25921 ccgagatgtt cgtaattgcc gaacgtaccg ctgcttttgg ctgcaaatcc tccaccaaaa
    25981 tcgataatgc cgactttaat caggcttaag ccgatcatca gtaccacaat accgctgacg
    26041 gtaggcgtaa taacccgacg taaatagggc aagataaatg aagatccgac aaccagaaat
    26101 gcgccaacga aggagacgcc gagaagcgac gacatgatta actcttcgtg aaaaccgtcg
    26161 cttttcatgc tgctgcccag cgcaatcatg accgtaacaa atgaaaaatt gactgactga
    26221 attgagagta ggccagaacc gacgatgccg tagcggttta cttgtaacca ggtaccaata
    26281 ccagaggcga tcatcgccat agaaacaaga taggcggttg tttcagcgga aagctgtaag
    26341 gccgcaccca cgattaacgc gggggttacc atcggaacga aaattgccaa cagatgggta
    26401 atggcaccaa cgagagcctg atgaaaggga gggcgatcct ccagttcaaa tataaggtca
    26461 gaacctgcat ggtttatatc agacatccct tcactccttt taaagtaaat ttgatcatct
    26521 gaattagtaa aataaattcc cacgaatttg gggcgcagga cgccttgaga acggcttatc
    26581 tcattttcgc agtcactatt tagtcatcgt tcaaacagag aaacaggccg ccgaagcggc
    26641 catttattac ttatattttt tccagcgcgg tgagtatttt ctccggtgtg aaatgccatt
    26701 cgcgtaacca gatgccgcag gcatcgtgaa ttgcggtagc aatcgccgga gctgcgccat
    26761 ttacaccaat ttccgagatc gatttcgccc cgaacgggcc gactttatcg tcgctcggca
    26821 ccaacacagc gcggaaatca cgcggaatgt caccaatttt cggtgcgccg taactgcgta
    26881 aatcacgcgt taacgggtga ccttcggcgt tataaatgat ctcttcgctc atactgtggc
    26941 cgatagcacg cagggtggca ccgtagattt gtcccaacgc taactctgga ttgaccggtg
    27001 taccgcagtc cagcaaggcg tagaatttat ccaggcggat ttcacccgta cgcgtgttga
    27061 cggcaacttc agcgaagttt gcgccatacg ggaaagcgaa atcaggcgtg atataactgc
    27121 cagttcccac cagtgaacca aagccggtgc cggtttcgcc tttatgggca atatccccga
    27181 aactaacttc gcctttcttg ccgcgcacga cgcccggcgt tgctagttga acatctgcca
    27241 ctggctcacc caacatttgc gcgccgtgga acagaatttt ctcccgcaga ttttccgctg
    27301 ccaaacgcgc cgcgttaccc gagaagcaag taccggacga ggcatatgcg cctttatcaa
    27361 acaacgcatg atcggtatca ccggagataa catgcacgtc ctgcggtggg cagtgcagca
    27421 cttctgctgc cagtttcgtc actacagtat ccagaccagt accaatatcc gcaccgccag
    27481 aatgaacgat aaaggtgccg tccgattcca gtttgatcat gcagttagcc tgatcgatat
    27541 ccgggatacc cgatttctgc atgataatgg cgacgccgcg accgatgtgc cagtcactat
    27601 tttgcggttt cggtgaagac cattggatca tctcgcgacc ctgacgcagg atctcttcca
    27661 gtgcgcagct ggcggcggaa ggaacggagg tcggcgcttt accttcaccg attgcaccga
    27721 gaattttcag ctcttgccct tcgtgtaccc ggttacgttc gataatttcc agttggtcga
    27781 tctgtaactg ttcagccagt tccgctaatg ccatggtgat agcgaagtta cctttcggtg
    27841 cgccataacc ctgataagca ccatttgggc aaatgttgct gtagtaggtg gtgacctgga
    27901 aatcgacgtt atcgcacgga tataacggca gcgacagcgc cggtccgtta cacggtacgg
    27961 tgagtgagtg gttgccgtaa gggccagtgt tggcgcggaa atccatcttc actgccgtca
    28021 ggcgaccatc ttttttcgct cccagtttga cggtgacttt cgcgacgtga cgagaggtgt
    28081 tagcaataaa ctcttcttca cgggtgtagc ggaacagtac cggacgcccg gtcacgcagg
    28141 ttgcccatgc gcacacttct tccagcagga tgtcctgttt ggagccaaaa ccgccgccaa
    28201 ctcgctcttt aatgacatga actttatgct gtttcatgcc tacgaggcgc gcgacctggc
    28261 ggcgtaagtg ccatggtacc tgggtggagg cgtggataac cagacgatcg ccgtccatcc
    28321 gggtaaagca gatatgtgtt tcagtcgggc actgctgtgc ctgcgttgag ttataggttc
    28381 gctcaatgat cacatcggca tcggcaaagc ctttgtccat atcgccaata tgaccatgaa
    28441 tactggcggc gatatttttg cgagggcgag aaccgatcgg gaagttgatg atcatatgct
    28501 cgccgcgctg ggctgcatgg ctgttatcgt cttccagagt atctggcgca ccagcaacat
    28561 acaccaccgg ttcatcgtgc acgacaggcg catcttccgc cattgcttcg tcgatcgaca
    28621 ttaccggctt aagcacttca tattcaacgt cgatgagctt caatgcttcg agcgcaattt
    28681 cttcactttc tgcgacgacc gcagcaacac gatcgccgac gtgacgcatt ttcttgccga
    28741 acatacggcg gtcaagcggt gacggttccg gtgcgctctg accacccggg gtatagtaga
    28801 tatccgggca attcaggtga gtaataacgt gaacgacgcc cggtaaggct tcagctttgc
    28861 tgacatccag atgagtaatc agtgcgtgag cgtgtgggct acgtaacatt ttaatgacgc
    28921 aggcgtcagc cgttacgcgg tcttcaacat agcagggttt cgcctgcacc attttcgcgg
    28981 catcagtttt aggataatgc ttgccaatga cttcaaggtc gtcacggaaa gtcggagcga
    29041 tatcaatggt ggcctgcgga ttatttttac gtgcaaccgc cagttcaatg acctgatagt
    29101 attgttgcca gcctgcatcg cggctgaaca aaccagaaag cgcgtcgtcg atctcttcgc
    29161 gggtaggtgc ggcgatgcga tcgagaagat cggtgataat cagagctgca gctggatcgt
    29221 tataaccaga ctgcaccacg ccaacatcaa ccattgcctg ttgaaccaga cttaactcgt
    29281 tccatttgcc caaagattct gcggtacgaa tatctgcctt ctctaactgt gcggcaataa
    29341 gcaaggacgc gttaacgata ttaccgttaa agattattgc gtcagaaccg gcaaacccga
    29401 agccatcatc actgttgcgt acagagtgca ttcccatgtt aaacaacagc ttttgcacgt
    29461 tttcgcctgg attaacggtt agctcctgag gcgcgccatt taaagtaaag tggatgatca
    29521 tacggcttcc tccccagcct gttggcagtc ggcatacagg tcggctacca gaactcccgt
    29581 gatatagcgt ttataggcca cgctgccccg caaatcttcc tgcgggaaaa tggcgttggc
    29641 gacagcctgt tcaagtgcat tgccttccag attttgtttt tcgacatcat gcagacgcag
    29701 tgctttactg gcgacgccat ccagcgcaat tcgcataccg tcgtggtctg tcattgcaac
    29761 ggctgccgtc acgacggtta aacctgcctg agaacggcta attttgcggg tcgcacaggt
    29821 gcgatacgga tctttaatga taatttcggt tagcaggcga tcgcatgggc aggccaggta
    29881 gtcctcgatt gacagcgttt cgccgttgcc aaaaaccagt tcagcatcca gtgccaacag
    29941 gacgggaagc agcaccgact cttcctggcg ggcggcgatt tcaccaccaa tggtcgactg
    30001 attacgaaca tggcgtgagt aaacaaaacc gagggcttca cgcagcgctg caggaataaa
    30061 tcgcgcatca cgcagtggct gcaagcgaga cattgcgcca atccgcagtg caccgttatc
    30121 ccagtcaacc cagtccagtt ccagatcctg taaggaaatg gcaatctttt tatcggtacg
    30181 ggttggtgta gcattgagtt tgctgccccc ggcgaaccag acggcttcat cctggtagcg
    30241 gcgcttcagt tccagcgcct gttcgacaga gtcgggcctg aaaaattgtt caatcatctt
    30301 ctttccttat ttaaccctct tccctgcggg aagagggcat ctgtcattta tgccagcgca
    30361 tccatccgac gccacatact ggcagcggct tttctggctt gtgcataaat ggaatcgcaa
    30421 tcgaagttaa actgacggtc ttcatagacc atcacaccat tgaccatcac gctgtgaacg
    30481 ctgcctgagc ccataccgaa agcgatatgc ccggcgatat tgtctgccag cagcggcgtc
    30541 ggtgagttgt aatcgcaaat ggttaaatca gctttgtaac cggcttccag aagtccaaat
    30601 ttcgcgccaa agttgcggct catcagttcg ttaccgttag tcagggcttt ggcaaaactg
    30661 tcaggccaca gcggaccacc cgcatcgcga tgtttaaaga aggcaaattt catctcttca
    30721 aacatgtccg aaccaatgcc gtccgttccc aacgccagat tgcggatgtc gctaaggtga
    30781 tggttgtagc cgacatggtt gttcatgttt gaacgggcgt tatgcaccag gaacgcatcg
    30841 cgctgattga gtagggcgat gtcatctttc gacaagtaca gcccatgagc gaccagcgtt
    30901 ttgctatcga tgagatcgaa ttgcgctagt cgtgccagca ggtctttgcc gtaccagtgg
    30961 tgactgtagg aaacgtcgta aaggtcttcc gcagcgtgaa tatgcaaacc acggcctgtg
    31021 gctttcacgg cttcacgtag catctccaga ccggcatctg gcacggtaaa cggcgcgtga
    31081 gcaccgatat gtgcttccac cagatacggc tcggttgctg ctttcttcgc ctcatcaatc
    31141 tgacgggcga agcggatgtt ttcttctaca ccttcctgca actctttgat gccgttgtta
    31201 cggtcagtag tttcaaaaca ggtcatcgcg cgcaggccaa cttttaaaaa tgcgtcgcgc
    31261 aatgtggaga gcgacccgcc gatatacgcc ggagaggcat ggtgatcgat aaccgatgta
    31321 catccgctct taatcgcttc cagggaacaa atcagcccgc tgtaatagag cgactcttca
    31381 tcaagggcgc gatcgagccg ccaccagaga tttttcagcg ttgagatgaa atccgggcag
    31441 ggggcgatgt ttgccataat tccgcgggaa agccccgagt aaaaatggtt gtgcgagcag
    31501 acaattcccg gcatcacaat ccgaccatgc atctctttgt agctggcatc ggggtagcgt
    31561 tgcgtcaggg catcgccgat agcgacaatc acatcatttt cgatagcgat atcaacgcct
    31621 tcctgcactt tcgccgggtg taactgcact gcggtgacat tcttcagaat caacatgatt
    31681 acacctccac gcggcccagc agataatgat gatgctggtg gacatggctg atgatgcggc
    31741 acatatcgtt cagctccggt ggtacgttgt tgaactgacc ttcgctgtcg atgtttaaca
    31801 cccagctttg gttattcagt cgtactcgta cccggcaatc ttccacaagg aagcctgggt
    31861 tgctgctgtt atcaaagtct tgcgccaggc tgaaaacggt gattttgtct ttgtatggtt
    31921 taccgttcca cggacagaac tgagcgcagt tgccgcattc gttacagtaa gcgtcgaggt
    31981 gcagcgtctg gaatctattc tggaagcctg ggaccgcaat ggagacgttg gcgcggttcg
    32041 ggcagacatc cacacacttg ctgcaaacgt agttacattc gaggcagcga gcggcttcct
    32101 gggcgacaaa cgcgtcacga tcgtcactgt tcaccagagt gatagagata tcgcctttac
    32161 gttgatagat ttccgccgga ttgacgttgt tccagtattt atcgttctgg tgggaacgga
    32221 tattttcccg actaaggatg gcatcggtcg cccgacgcgc ggttccgaca gcagcgacaa
    32281 tggaggatgg tccgcgctgt acgtcgccga tcataaagac gtcagtcaga cgagtttcgc
    32341 cattgtggtc gacgtctggc cagccgtttt tgtccagcgg cacgcccatc gcattcaggg
    32401 cttcagtatc ctgctgttca ccaatggcgg tgatcaggct gtctacatgc agtgttactg
    32461 tttcgttggt ttcaaccgga cggcgacggc ctttctcatc gggttcgcca agcgacataa
    32521 cgcgcaaggt taaggtgcca tcagcatcga aacgttccgg attattcagg aaacggaact
    32581 ccacgccgtc gtgcaacgct tcttcatact cttcgcgcca tgcgggcatc tcttgcagtg
    32641 aacgacggta aacgatcgtt gctttttcta cgcctggaac gcgtaacgcc gcacgagcgc
    32701 agtccattgc ggtgttaccc gccccgacaa cgaccacatg tttgcccagc ttgagcgctg
    32761 tacccttgtt gtattcacgc aggaagggga gtgatttcca aacattttgg ttgtcacccg
    32821 ccagtttcac accgctattt ttatcagtgc cggtggcaat cagaacatag tggaagccct
    32881 gattttttaa ctgttcaacg gtcaaatcgg gtgagcagcc atactcaaat ttcacgccgt
    32941 gatcggcaac aaaatcgata tcgtgctgaa ttaactcagc cggaattcgg aactgaggaa
    33001 tgatattttt caccacgccg cccgcattgg cttcgcgttc aaacagcgta accggatggc
    33061 ccgctctggc aaggaagtaa cctgctgcca gacccgccgg acctgcacca atcacggcaa
    33121 ccggatggcg tgaaccagaa ccggctggtt tgtgccagcg ttgcttatat tcatcccaac
    33181 ctttttccag cgcgactttt ttcagttcgc ggatattcag cgcactgtcg taatccaggc
    33241 gggtacagtt gtattggcac tggtgatcgc aaatatgacc ggtaatggcg ggcagagcgt
    33301 tgcgttggta gatgagttcc agcgcgtcgg catagcggtg ttcgccaagc agacggatgt
    33361 attccggaat atcttgcttg atagcgcagg cagtaacaca gggggcaacg tagcaatcgg
    33421 tcagcggcag gtcttctgcc acttcaatac gctcttctgg cttccagtgt ttctgggtgt
    33481 attccatggt taacgcatct gctgccagtc tgttcagtcg ttcgacgtca acatggtcaa
    33541 gtccccaggc gtcggagcct tccagctcgc gcatgcaggc acttaagcgc aaatagccgc
    33601 caggtttcag caggtcggtt gccatagtaa tagggcgaat acctgtatca aaaatatcgc
    33661 ggatagtcag ctgactggca ccaccggaat aagaaatggg cagtttgccg tcaaaggcgc
    33721 gagagagaac tgctgcaaca ttgatggaga gcgggaacag cgcacggcct gacatataca
    33781 tctcttcacc aggcagtgcg cctttattgt tgatggtgcc gagagtgtta gtcagtttta
    33841 cgccaaagcc gagtgatttt tcttttgcca gtgccatcag gcgttccagc atttccagtg
    33901 cttgcgtcag cttgaggtcg tgatcaaatg actcttcttt taagcctatg taaccgaaac
    33961 cgcagacatc gaggatctca cgaacacgcg cgtatcccag taaggtcggg ttaagtttca
    34021 caaaggtgtt gagccctttt tcttccagca tgtagcggca aatggcttcg atttcatgcg
    34081 gaggacagcc gtgcatggtg gagagggtga cgccctgcac catactggtg gggatgcgag
    34141 cgggtaaggc ttgcaagctt tcgcgttttt cctgcaatcc gtggcgggct aaaaatgcgt
    34201 catcctggag taatttattc agcgtatcgc gatattgcgc gaatttcgga tggtcagatg
    34261 cgtccatcat attgtcgatg aactgttgca ttggcggttg cttaatacct tcgaggttgt
    34321 aaccgacgct catattaaag atgaacgatt taccggaatc agaaggctgg aacatcgctt
    34381 cgagaaggtg cagggcaaac caggctttga ggtattcatc ccaggcttta agcagggtaa
    34441 attcggtaga ccattcggtg ttaaagcact cgtcttcggc atcgatacag ggcttttcca
    34501 gctccaggcg gtcaagaatt tggacggttt ttagttcgat gaagcgtccg ccagtcagcc
    34561 aggaagtgac aatattttgc gcgagctgcg tgtgcgggcc agcggcaggg ccgacgggag
    34621 tggcacaggt ttcaccgaag acgctaacag ttttaccttt tacaggtgag taaaactgtt
    34681 gctcgggaat accaaagatt gagcgttgtt gttggtattc atcaaatatg cgcgtcaaaa
    34741 gttcctcaaa cggaatggga cgcataatat cccccataac cctctcctta gcttatacag
    34801 atatcattga gtgaccggtg ttgagaaatc cggtggttta gggggataga gcaacaatcg
    34861 caccactctt gccaggtttg gcgggaaata taaaaaaatg tgaggcggct cgaaacttaa
    34921 aggctttttg tgaatgaact cacaggtgcc aaatgagaat taaaatcact cctgaaaatt
    34981 attattgtca gcccatttat cactttttct cttttctcaa aatcagtga
//
LOCUS       DAZKHF020000019        34517 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2201S31241
            SAMN43267800-rid25106413.denovo.019, whole genome shotgun sequence.
ACCESSION   DAZKHF020000019 DAZKHF020000000
VERSION     DAZKHF020000019.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267800
            Sequence Read Archive: SRR30307263
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 34517)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 34517)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 34517)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350581.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350581.2
            Genome Coverage       :: 151x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:21:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,652
            CDSs (total)                      :: 5,557
            Genes (coding)                    :: 5,320
            CDSs (with protein)               :: 5,320
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 237
            CDSs (without protein)            :: 237
            Pseudo Genes (ambiguous residues) :: 0 of 237
            Pseudo Genes (frameshifted)       :: 102 of 237
            Pseudo Genes (incomplete)         :: 130 of 237
            Pseudo Genes (internal stop)      :: 52 of 237
            Pseudo Genes (multiple problems)  :: 41 of 237
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..34517
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267800-rid25106413.denovo.019"
                     /serotype="O 26:H11"
                     /isolate="2201S31241"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-01"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            complement(<1..1236)
                     /gene="amiC"
                     /locus_tag="ACHOSC_RS03950"
                     /old_locus_tag="pgaptmp_000790"
     CDS             complement(<1..1236)
                     /gene="amiC"
                     /locus_tag="ACHOSC_RS03950"
                     /old_locus_tag="pgaptmp_000790"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311701.2"
                     /note="Like AmiA, dependent on TAT (Twin-Arginine
                     Translocation) system for export; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase AmiC"
                     /protein_id="HGU4546780.1"
                     /translation="MSGSNTAISRRRLLQGAGAMWLLSVSQVSLAAVSQVVAVRVWPA
                     SSYTRVTVESNRQLKYKQFALSNPERVVVDIEDVNLNSVLKGMAAQIRADDPFIKSAR
                     VGQFDPQTVRMVFELKQNVKPQLFALAPVAGFKERLVMDLYPANAQDMQDPLLALLED
                     YNKGDLEKQVPPAQSGPQPGKAGRDRPIVIMLDPGHGGEDSGAVGKYKTREKDVVLQI
                     ARRLRSLIEKEGNMKVYMTRNEDIFIPLQVRVAKAQKQRADLFVSIHADAFTSRQPSG
                     SSVFALSTKGATSTAAKYLAQTQNASDLIGGVSKSGDRYVDHTMFDMVQSLTIADSLK
                     FGKAVLNKLGKINKLHKNQVEQAGFAVLKAPDIPSILVETAFISNVEEERKLKTATFQ
                     QEVAESILAGIKAYFADGAT"
     gene            1468..2799
                     /gene="argA"
                     /locus_tag="ACHOSC_RS03955"
                     /old_locus_tag="pgaptmp_000791"
     CDS             1468..2799
                     /gene="argA"
                     /locus_tag="ACHOSC_RS03955"
                     /old_locus_tag="pgaptmp_000791"
                     /EC_number="2.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004042 - acetyl-CoA:L-glutamate
                     N-acetyltransferase activity [Evidence IEA];
                     GO_process: GO:0006526 - arginine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino-acid N-acetyltransferase"
                     /protein_id="HGU4546781.1"
                     /translation="MVKERKTELVEGFRHSVPYINTHRGKTFVIMLGGEAIEHENFSS
                     IVNDIGLLHSLGIRLVVVYGARPQIDANLAAHHHEPLYHKNIRVTDAKTLELVKQAAG
                     TLQLDITARLSMSLNNTPLQGAHINVVSGNFIIAQPLGVDDGVDYCHSGRIRRIDEDA
                     IHRQLDSGAIVLMGPVAVSVTGESFNLTSEEIATQLAIKLKAEKMIGFCSSQGVTNDD
                     GDIVSELFPNEAQARVEAQEEKGDYNSGTVRFLRGAVKACRSGVRRCHLISYQEDGAL
                     LQELFSRDGIGTQIVMESAEQIRRATINDIGGILELIRPLEQQGILVRRSREQLEMEI
                     DKFTIIQRDNTTIACAALYPFPEEKIGEMACVAVHPDYRSSSRGEVLLERIAAQAKQS
                     GLSKLFVLTTRSIHWFQERGFTPVDIDLLPESKKQLYNYQRKSKVLMADLG"
     gene            complement(2861..4687)
                     /gene="recD"
                     /locus_tag="ACHOSC_RS03960"
                     /old_locus_tag="pgaptmp_000792"
     CDS             complement(2861..4687)
                     /gene="recD"
                     /locus_tag="ACHOSC_RS03960"
                     /old_locus_tag="pgaptmp_000792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009338 - exodeoxyribonuclease V complex
                     [Evidence IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0008854 - exodeoxyribonuclease V activity
                     [Evidence IEA];
                     GO_process: GO:0006302 - double-strand break repair
                     [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease V subunit alpha"
                     /protein_id="HGU4546782.1"
                     /translation="MKLQKQLLEAVEHKQLRPLDVQFALTVAGDEHPAVTLAAALLSH
                     DAGEGHVCLPLSRLENNEASHPLLATCVSEIGELQNWEECLLASQAVSRGDEPTPMVL
                     CGDRLYLNRMWCNERTVARFFNEVNHAIEVDEALLAQTLDKLFPVSDEINWQKVAAAV
                     ALTRRISVVSGGPGTGKTTTVAKLLAALIQMADGERCRIRLAAPTGKAAARLTESLGK
                     ALRQLPLTDEQKKRIPEDASTLHRLLGAQPGSQRLRHHAGNPLHLDVLVVDEASMIDL
                     PMMSRLIDALPDHARVIFLGDRDQLASVEAGAVLGDICAYANAGFTAERARQLSRLTG
                     THVPAGTGTEAASLRDSLCLLQKSYRFGSDSGIGQLAAAINRGDKTAVKTVFQQDFTD
                     IEKRLLQSGEDYIAMLEEALAGYGRYLDLLQARAEPDLIIQAFNEYQLLCALREGPFG
                     VAGLNERIEQFMQQKRKIHRHPHSRWYEGRPVMIARNDSALGLFNGDIGIALDRGQGT
                     RVWFAMPDGNIKSVQPSRLPEHETTWAMTVHKSQGSEFDHAALILPSQRTPVVTRELV
                     YTAVTRARRRLSLYADERILSAAIATRTERRSGLAALFSSRE"
     gene            complement(4687..8229)
                     /gene="recB"
                     /locus_tag="ACHOSC_RS03965"
                     /old_locus_tag="pgaptmp_000793"
     CDS             complement(4687..8229)
                     /gene="recB"
                     /locus_tag="ACHOSC_RS03965"
                     /old_locus_tag="pgaptmp_000793"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708611.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008854 - exodeoxyribonuclease V activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease V subunit beta"
                     /protein_id="HGU4546783.1"
                     /translation="MSDVAETLDPLRLPLQGERLIEASAGTGKTFTIAALYLRLLLGL
                     GGSAAFPRPLTVEELLVVTFTEAATAELRGRIRSNIHELRIACLRETTDNPLYERLLE
                     EIDDKAQAAQWLLLAERQMDEAAVFTIHGFCQRMLNLNAFESGMLFEQQLIEDESLLR
                     YQACADFWRRHCYPLPREIALVVFETWKGPQALLRDINRYLQGEAPVIKAPPPDDETL
                     ASRHAQIVARIDTVKQQWRDAVGELDALIESSGIDRRKFNRSNQAKWIEKISAWAEEE
                     TNSYQLPESLEKFSQRFLEDRTKAGGETPRHPLFEAIDQLLAEPLSIRDLVITRALAE
                     IRETVAREKRRRGELGFDDMLSRLDSALRSESGEVLAAAIRTRFPVAMIDEFQDTDPQ
                     QYRIFRRIWHHQPETALLLIGDPKQAIYAFRGADIFTYMKARSEVHAHYTLDTNWRSA
                     PGMVNSVNKLFSQTDDAFMFREIPFIPVKSAGKNQALRFVFKGETQPAMKMWLMEGES
                     CGVGDYQSTMAQVCAAQIRDWLQAGQRGEALLMNGDDARPVRASDISVLVRSRQEAAQ
                     VRDALTLLEIPSVYLSNRDSVFETLEAQEMLWLLQAVMTPERENTLRSALATSMMGLN
                     ALDIETLNNDEHAWDAVVEEFDGYRQIWRKRGVMPMLRALMSARNIAENLLATAGGER
                     RLTDILHISELLQEAGTQLESEHALVRWLSQHILEPDSNASSQQMRLESDKHLVQIVT
                     IHKSKGLEYPLVWLPFITNFRVQDQAFYHDRHSFEAVLDLNAAPESVELAEVERLAED
                     LRLLYVALTRSVWHCSLGVAPLVRRRGDKKGDTDVHQSALGRLLQKGEPQDAAGLRTC
                     IEALCDDDIAWQTAQTGDNQPWQVNDALTAELNARTLQRLPGDNWRVTSYSGLQQRGH
                     GIAQDLMPRLDVDAAGVVSVVEEPTLTPHQFPRGASPGTFLHSLFEDLDFTQPVDPNW
                     VQEKLELGGFESQWEPVLTEWITAVLQAPLNETGVSLSQLSDRDKQVEMEFYLPISEP
                     LIASQLDALIRQFDPLSAGCPPLEFMQVRGMLKGFIDLVFRHEGRYYLLDYKSNWLGE
                     DSSAYTQQAMAAAMQAHRYDLQYQLYTLALHRYLRHRIADYDYDRHFGGVIYLFLRGV
                     DKEHPQQGIYTTRPNAGLIALMDEMFAGMTLEEA"
     gene            complement(8222..11110)
                     /gene="ptrA"
                     /locus_tag="ACHOSC_RS03970"
                     /old_locus_tag="pgaptmp_000794"
     CDS             complement(8222..11110)
                     /gene="ptrA"
                     /locus_tag="ACHOSC_RS03970"
                     /old_locus_tag="pgaptmp_000794"
                     /EC_number="3.4.24.55"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pitrilysin"
                     /protein_id="HGU4546784.1"
                     /translation="MPRSTWFKALLLLVALWAPLSQAETGWQPIQETIRKSDKDNRQY
                     QAIRLDNGMVVLLVSDPQAVKSLSALVVPVGSLEDPEAYQGLAHYLEHMSLMGSKKYP
                     QADSLAEYLKMHGGSHNASTAPYRTAFYLEVENDALPGAVDRLADAIAEPLLDKKYAE
                     RERNAVNAELTMARTRDGMRMAQVSAETINPAHPGSKFSGGNLETLSDKPGNPVQQAL
                     KDFHEKYYSANLMKAVIYSNKPLPELAKMAADTFGRVPNKESKKPEITVPVVTDAQKG
                     IIIHYVPALPRKVLRVEFRIDNNSAKFRSKTDELITYLIGNRSPGTLSDWLQKQGLVE
                     GISANSDPIVNGNSGVLAISASLTDKGLANRDQVVAAIFSYLNLLREKGIDKQYFDEL
                     ANVLDIDFRYPSITRDMDYVEWLADTMIRVPVEHTLDAVNIADRYDAKAVKERLAMMT
                     PQNARIWYISPKEPHNKTAYFVDAPYQVDKISAQTFADWQKKAADIALSLPELNPYIP
                     DDFSLIKSDKKYDHPELIVDESNLRVVYAPSRYFASEPKADVSLILRNPKAMDSARNQ
                     VMFALNDYLAGLALDQLSNQASVGGISFSTNANNGLMVNANGYTQHLPQLFQALLEGY
                     FSYTATEDQLEQAKSWYNQMMDSAEKGKAFEQAIMPAQMLSQVPYFSRDERRKILPSI
                     TLKEVLAYRDALKSGARPEFMVIGNMTEDQATTLARDVQKQLGADGSEWCRNKDVVVD
                     KKQSVIFEKAGNSTDSALAAVFVPTGYDEYTSSAYSSLLGQIVQPWFYNQLRTEEQLG
                     YAVFAFPMSVGRQWGMGFLLQSNDKQPSFLWERYKAFFPTAEAKLRAMKPEEFAQIQQ
                     AVITQMLQAPQTLGEEASKLSKDFDRGNMRFDSRDKIVAQIKLLTPQKLADFFHLAVV
                     EPQGMAILSQISGSQNGKAEYVHPEGWKVWENVSALQQTMPLMSEKNE"
     gene            complement(11286..14654)
                     /gene="recC"
                     /locus_tag="ACHOSC_RS03975"
                     /old_locus_tag="pgaptmp_000795"
     CDS             complement(11286..14654)
                     /gene="recC"
                     /locus_tag="ACHOSC_RS03975"
                     /old_locus_tag="pgaptmp_000795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009338 - exodeoxyribonuclease V complex
                     [Evidence IEA];
                     GO_function: GO:0008854 - exodeoxyribonuclease V activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease V subunit gamma"
                     /protein_id="HGU4546785.1"
                     /translation="MLRVYHSNRLDVLEALMEFIVERERLDDPFEPEMILVQSTGMAQ
                     WLQMTLSQKFGIAANIDFPLPASFIWDMFVRVLPEIPKESAFNKQSMSWKLMTLLPQL
                     LDREDFTLLRHYLADDSDKRKLFQLSSKAADLFDQYLVYRPEWLAQWETGHLVEGLGE
                     AQAWQAPLWKALVEYTHELGQPRWHRANLYQRFIETLESATTCPPGLPSRVFICGISA
                     LPPVYLQALQALGKHIEIHLLFTNPCRYYWGDIKDPAYLAKLLTRQRRHSVEDRELPL
                     FRDSENAGQLFNSDGEQDVGNPLLASWGKLGRDYIYLLSDLESSQELDAFVDVTPDNL
                     LHNIQSDILELENRAVAGVNIEEFSRSDNKRPLDPLDSSITFHVCHSPQREVEVLHDR
                     LLAMLEEDPTLTPRDIIVMVADIDSYSPFIQAVFGSAPADRYLPYAISDRRARQSHPV
                     LEAFISLLSLPDSRFVSEDVLALLDVPVLAARFDITEEGLRYLRQWVNESGIRWGIDD
                     DNVRELELPATGQHTWRFGLTRMLLGYAMESAQGEWQSVLPYDESSGLIAELVGHLAS
                     LLMQLNIWRRGLAQERPLEEWLPVCRDMLNAFFLPDAETEAAMTLIEQQWQAIIAEGL
                     GAQYGDAVPLSLLRDELAQRLDQERISQRFLAGPVNICTLMPMRSIPFKVVCLLGMND
                     GVYPRQLAPLGFDLMSQKPKRGDRSRRDDDRYLFLEALISAQQKLYISYIGRSIQDNS
                     ERFPSVLVQELIDYIGQSHYLPDDEALNCDESEARVKAHLTCLHTRMPFDPQNYQPGE
                     RQSYAREWLPAASQAGKAHSEFVQPLPFTLPETVPLETLQRFWAHPVRAFFQMRLQVN
                     FRTEDSEIPDTEPFILEGLSRYQINQQLLNALVEQDDAERLFRRFRAAGDLPYGAFGE
                     IFWETQCQEMQQLADRVIACRQPGQSMEIDLACNGVQITGWLPQVQPDGLLRWRPSLL
                     SVAQGMQLWLEHLVYCASGGNGESRLFLRKDGEWRFPPLAAEQALHYLSQLIEGYREG
                     MSAPLLVLPESGGAWLKTCYDAQNDAMLDDDSTLQKARTKFLQAYEGNMMVRGEGDDI
                     WYQRLWRQLTPETMEAIVEQSQRFLLPLFRFNQS"
     gene            complement(14667..14990)
                     /locus_tag="ACHOSC_RS03980"
                     /old_locus_tag="pgaptmp_000796"
     CDS             complement(14667..14990)
                     /locus_tag="ACHOSC_RS03980"
                     /old_locus_tag="pgaptmp_000796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HGU4546786.1"
                     /translation="MSASLKNQQGFSLPEVMVAMVLMVMIVTALSGIQRTLMNSLASR
                     NQYQQLWRHGWQQTQLRAISPPANWQVNRMQTSQAGCVSISVTLVSPGGREGEMTRLH
                     CPNRQ"
     gene            complement(14975..15382)
                     /locus_tag="ACHOSC_RS03985"
                     /old_locus_tag="pgaptmp_000797"
     CDS             complement(14975..15382)
                     /locus_tag="ACHOSC_RS03985"
                     /old_locus_tag="pgaptmp_000797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2509 family protein"
                     /protein_id="HGU4546787.1"
                     /translation="MNREKGVSSLALVLMLLVLGSLLLQGMSQQDRSFASRVSMESQS
                     LRRQAIVQSALAWGKMHSWQTQPAVQCSQYAGTDARVCLRLLADNEALLVAGYEGVSL
                     WRTGEVIDGNIVFSPRGWSDFCPLKERALCQLP"
     gene            complement(15379..15942)
                     /locus_tag="ACHOSC_RS03990"
                     /old_locus_tag="pgaptmp_000798"
     CDS             complement(15379..15942)
                     /locus_tag="ACHOSC_RS03990"
                     /old_locus_tag="pgaptmp_000798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase-dependent protein"
                     /protein_id="HGU4546788.1"
                     /translation="MPVKEQGFSLLEVLIAMAISSVLLLGAARFLPALQRESLTSTRK
                     LALEDEIWLRVFTVAKHLQRAGYCHGSCTGEGLEIVGQGDCIIVQWDANSNGIWDREP
                     VKESDQIGFRLKEHVLETLRGATSCEGKGWDKVTNPDAIIIDTFQVVRQDVSGFSPVL
                     TVNMRAASKSEPQTVVDASYSVTGFNL"
     gene            complement(15933..16403)
                     /locus_tag="ACHOSC_RS03995"
                     /old_locus_tag="pgaptmp_000799"
     CDS             complement(15933..16403)
                     /locus_tag="ACHOSC_RS03995"
                     /old_locus_tag="pgaptmp_000799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase-dependent protein"
                     /protein_id="HGU4546789.1"
                     /translation="MKTQRGYTLIETLVAMLILVMLSASGLYGWQYWQQSQRLWQTAS
                     QARDYLLYLREDANWHNRDHSISVIREGTLWCLVSSAAGANTCHGSSPLVFVPRWPEV
                     EMSDLTPSLAFFGLRNTAWAGHIRFKNSTGEWWLVVSPWGRLRLCQQGETEGCL"
     gene            complement(16587..17381)
                     /gene="thyA"
                     /locus_tag="ACHOSC_RS04000"
                     /old_locus_tag="pgaptmp_000800"
     CDS             complement(16587..17381)
                     /gene="thyA"
                     /locus_tag="ACHOSC_RS04000"
                     /old_locus_tag="pgaptmp_000800"
                     /EC_number="2.1.1.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004799 - thymidylate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006231 - dTMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thymidylate synthase"
                     /protein_id="HGU4546790.1"
                     /translation="MKQYLELMQKVLDEGTQKNDRTGTGTLSIFGHQMRFNLQDGFPL
                     VTTKRCHLRSIIHELLWFLQGDTNIAYLHENNVTIWDEWADENGDLGPVYGKQWRAWP
                     TPDGRHIDQITTVLNQLKNDPDSRRIIVSAWNVGELDKMALAPCHAFFQFYVADGKLS
                     CQLYQRSCDVFLGLPFNIASYALLVHMMAQQCDLEVGDFVWTGGDTHLYSNHMDQTHL
                     QLSREPRPLPKLIIKRKPESIFDYRFEDFEIEGYDPHPGIKAPVAI"
     gene            complement(17388..18263)
                     /gene="lgt"
                     /locus_tag="ACHOSC_RS04005"
                     /old_locus_tag="pgaptmp_000801"
     CDS             complement(17388..18263)
                     /gene="lgt"
                     /locus_tag="ACHOSC_RS04005"
                     /old_locus_tag="pgaptmp_000801"
                     /EC_number="2.5.1.145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008961 -
                     phosphatidylglycerol-prolipoprotein diacylglyceryl
                     transferase activity [Evidence IEA];
                     GO_process: GO:0009249 - protein lipoylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolipoprotein diacylglyceryl transferase"
                     /protein_id="HGU4546791.1"
                     /translation="MTSSYLHFPEFDPVIFSIGPVALHWYGLMYLVGFIFAMWLATRR
                     ANRPGSGWTKNEVENLLYAGFLGVFLGGRIGYVLFYNFPQFMADPLYLFRVWDGGMSF
                     HGGLIGVIVVMIIFARRTKRSFFQVSDFIAPLIPFGLGAGRLGNFINGELWGRVDPNF
                     PFAMLFPGSRTEDILLLQTNPQWQSIFDTYGVLPRHPSQLYELLLEGVVLFIILNLYI
                     RKPRPMGAVSGLFLIGYGAFRIIVEFFRQPDAQFTGAWVQYISMGQILSIPMIVAGVI
                     MMVWAYRRSPQQHVS"
     gene            complement(18414..20660)
                     /gene="ptsP"
                     /locus_tag="ACHOSC_RS04010"
                     /old_locus_tag="pgaptmp_000802"
     CDS             complement(18414..20660)
                     /gene="ptsP"
                     /locus_tag="ACHOSC_RS04010"
                     /old_locus_tag="pgaptmp_000802"
                     /EC_number="2.7.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate--protein phosphotransferase"
                     /protein_id="HGU4546792.1"
                     /translation="MLTRLREIVEKVASAPRLNEALNILVTDICLAMDTEVCSVYLAD
                     HDRRCYYLMATRGLKKPRGRTVTLAFDEGIVGLVGRLAEPINLADAQKHPSFKYIPSV
                     KEERFRAFLGVPIIQRRQLLGVLVVQQRELRQYDESEESFLVTLATQMAAILSQSQLT
                     ALFGQYRQTRIRALPAAPGVAIAEGWQDATLPLMEQVYQASTLDPALERERLTGALEE
                     AANEFRRYSKRFAAGAQKETAAIFDLYSHLLSDTRLRRELFAEVDKGSVAEWAVKTVI
                     EKFAEQFAALSDNYLKERAGDLRALGQRLLFHLDDANQGPNAWPERFILVADELSATT
                     LAELPQDRLVGVVVRDGAANSHAAIMVRALGIPTVMGADIQPSVLHRRTLIVDGYRGE
                     LLVDPEPVLLQEYQRLISEEIELSRLAEDDVNLPAQLKSGERIKVMLNAGLSPEHEEK
                     LGSRIDGIGLYRTEIPFMLQSGFPSEEEQVAQYQGMLQMFNDKPVTLRTLDVGADKQL
                     PYMPISEENPCLGWRGIRITLDQPEIFLIQVRAMLRANAATGNLNILLPMVTSLDEVD
                     EARRLIERAGREVEEMIGYEIPKPRIGIMLEVPSMVFMLPHLAKRVDFISVGTNDLTQ
                     YILAVDRNNTRVANIYDSLHPAMLRALAMIAREAEIHGIDLRLCGEMAGDPMCVAILI
                     GLGYHHLSMNGRSVARVKYLLRRIDYAEAENLAQRSLEAQLATEVRHQVAAFMERRGM
                     GGLIRGGL"
     gene            complement(20673..21203)
                     /gene="rppH"
                     /locus_tag="ACHOSC_RS04015"
                     /old_locus_tag="pgaptmp_000803"
     CDS             complement(20673..21203)
                     /gene="rppH"
                     /locus_tag="ACHOSC_RS04015"
                     /old_locus_tag="pgaptmp_000803"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001655775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA pyrophosphohydrolase"
                     /protein_id="HGU4546793.1"
                     /translation="MIDDDGYRPNVGIVICNRQGQVMWARRFGQHSWQFPQGGINPGE
                     SAEQAMYRELFEEVGLSRKDVRILASTRNWLRYKLPKRLVRWDTKPVCIGQKQKWFLL
                     QLVSGDAEINMQTSSTPEFDGWRWVSYWYPVRQVVSFKRDVYRRVMKEFASVVMSLQE
                     NTPKPQNASAYRRKRG"
     gene            complement(21556..21702)
                     /gene="ygdT"
                     /locus_tag="ACHOSC_RS04020"
                     /old_locus_tag="pgaptmp_000804"
     CDS             complement(21556..21702)
                     /gene="ygdT"
                     /locus_tag="ACHOSC_RS04020"
                     /old_locus_tag="pgaptmp_000804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YgdT"
                     /protein_id="HGU4546794.1"
                     /translation="MLSTESWDNCEKPPLLFPFTALTCDETPVFSGSVLNLVAHSVDK
                     YGIG"
     gene            21888..22577
                     /gene="mutH"
                     /locus_tag="ACHOSC_RS04025"
                     /old_locus_tag="pgaptmp_000805"
     CDS             21888..22577
                     /gene="mutH"
                     /locus_tag="ACHOSC_RS04025"
                     /old_locus_tag="pgaptmp_000805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA];
                     GO_process: GO:0006304 - DNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair endonuclease MutH"
                     /protein_id="HGU4546795.1"
                     /translation="MSQPRPLLSPPETEEQLLAQAQQLSGYTLGELAALAGLVTPENL
                     KRDKGWIGVLLEIWLGASAGSKPEQDFAALGVELKTIPVDSLGRPLETTFVCVAPLTG
                     NSGVTWETSHVRHKLKRVLWIPVEGERSIPLAQRRVGSPLLWSPNEEEDRQLREDWEE
                     LMDMIVLGQVERITARHGEYLQIRPKAANAKALTEAIGARGERILTLPRGFYLKKNFT
                     SALLARHFLIQ"
     gene            22646..23359
                     /locus_tag="ACHOSC_RS04030"
                     /old_locus_tag="pgaptmp_000806"
     CDS             22646..23359
                     /locus_tag="ACHOSC_RS04030"
                     /old_locus_tag="pgaptmp_000806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TerC family protein"
                     /protein_id="HGU4546796.1"
                     /translation="MLFAWITDPNAWLALGTLTLLEIVLGIDNIIFLSLVVAKLPTAQ
                     RAHARRLGLAGAMVMRLALLASIAWVTRLTNPLFTIFSQEISARDLILLLGGLFLIWK
                     ASKEIHESIEGEEEGLKTRVSSFLGAIVQIMLLDIIFSLDSVITAVGLSDHLFIMMAA
                     VVIAVGVMMFAARSIGDFVERHPSVKMLALSFLILVGFTLILESFDIHVPKGYIYFAM
                     FFSIAVESLNLIRNKKNPL"
     gene            23497..23715
                     /gene="ygdR"
                     /locus_tag="ACHOSC_RS04035"
                     /old_locus_tag="pgaptmp_000807"
     CDS             23497..23715
                     /gene="ygdR"
                     /locus_tag="ACHOSC_RS04035"
                     /old_locus_tag="pgaptmp_000807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein YgdR"
                     /protein_id="HGU4546797.1"
                     /translation="MKKWAVIISAVGLAFAVSGCSSDYVMATKDGRMILTDGKPEIDD
                     DTGLVSYHDQQGNAMQINRDDVSQIIER"
     gene            23823..24863
                     /locus_tag="ACHOSC_RS04040"
                     /old_locus_tag="pgaptmp_000808"
     CDS             23823..24863
                     /locus_tag="ACHOSC_RS04040"
                     /old_locus_tag="pgaptmp_000808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047834 - D-threo-aldose 1-dehydrogenase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADP(H)-dependent aldo-keto reductase"
                     /protein_id="HGU4546798.1"
                     /translation="MQYHRIPHSSLEVSTLGLGTMTFGEQNSEADAHAQLDYAVAQGI
                     NLIDVAEMYPVPPRPETQGLTETYVGNWLAKHGSREKLIIASKVSGPSRNNDKGIRPD
                     QALDRKNIREALHDSLKRLQTDYLDLYQVHWPQRPTNCFGKLGYSWTDSAPAVSLLDT
                     LDALAEYQRAGKIRYIGVSNETAFGVMRYLHLADKHDLPRIVTIQNPYSLLNRSFEVG
                     LAEVSQYEGVELLAYSCLGFGTLTGKYLNGAKPAGARNTLFSRFTRYSGEQTQKAVAA
                     YVDIARRHGLAPAQMALAFVRRQPFVASTLLGATTMDQLKTNIESLHLELSEDVLAEI
                     EAVHQVYTYPAP"
     gene            complement(24895..26088)
                     /gene="lplT"
                     /locus_tag="ACHOSC_RS04045"
                     /old_locus_tag="pgaptmp_000809"
     CDS             complement(24895..26088)
                     /gene="lplT"
                     /locus_tag="ACHOSC_RS04045"
                     /old_locus_tag="pgaptmp_000809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid transporter LplT"
                     /protein_id="HGU4546799.1"
                     /translation="MSESVHTNTSLWSKGMKAVIVAQFLSAFGDNALLFATLALLKAQ
                     FYPEWSQPILQMVFVGAYILFAPFVGQVADSFAKGRVMMFANGLKLLGAASICFGINP
                     FLGYTLVGVGAAAYSPAKYGILGELTTGSKLVKANGLMEASTIAAILLGSVAGGVLAD
                     WHVLVALAACALAYGGAVVANIYIPKLAAARPGQSWNLINMTRSFLNACTSLWRNGET
                     RFSLVGTSLFWGAGVTLRFLLVLWVPVALGITDNATPTYLNAMVAIGIVVGAGAAAKL
                     VTLETVSRCMPAGILIGVVVLIFSLQHELLPAYALLMLIGVMGGFFVVPLNALLQERG
                     KKSVGAGNAIAVQNLGENSAMLLMLGIYSLAVMIGIPVVPIGIGFGALFALAITALWI
                     WQRRH"
     gene            complement(26081..28240)
                     /gene="aas"
                     /locus_tag="ACHOSC_RS04050"
                     /old_locus_tag="pgaptmp_000810"
     CDS             complement(26081..28240)
                     /gene="aas"
                     /locus_tag="ACHOSC_RS04050"
                     /old_locus_tag="pgaptmp_000810"
                     /EC_number="2.3.1.40"
                     /EC_number="6.2.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008779 -
                     acyl-[acyl-carrier-protein]-phospholipid O-acyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0008922 - long-chain fatty acid
                     [acyl-carrier-protein] ligase activity [Evidence IEA];
                     GO_process: GO:0006631 - fatty acid metabolic process
                     [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional acyl-ACP--phospholipid
                     O-acyltransferase/long-chain-fatty-acid--ACP ligase"
                     /protein_id="HGU4546800.1"
                     /translation="MLFSFFRNLCRVLYRVRVTGDTQALKGERVLITPNHVSFIDGIL
                     LGLFLPVRPVFAVYTSISQQWYMRWLKSFIDFVPLDPTQPMAIKHLVRLVEQGRPVVI
                     FPEGRITTTGSLMKIYDGAGFVAAKSGATVIPVRIEGAELTHFSRLKGLVKRRLFPQI
                     TLHILPPTQVAMPDAPRARDRRKIAGEMLHQIMMEARMAVRPRETLYESLLSAMYRFG
                     AGKKCVEDVNFTPDSYRKLLTKTLFVGRILEKYSVEGERIGLMLPNAGISAAVIFGAI
                     ARRRIPAMMNYTAGVKGLTSAITAAEIKTIFTSRQFLDKGKLWHLPEQLTQVRWVYLE
                     DLKADVTTADKVWIFAHLLMPRLAQVKQQPEEEALILFTSGSEGHPKGVVHSHKSILA
                     NVEQIKTIADFTTNDRFMSALPLFHSFGLTVGLFTPLLTGAEVFLYPSPLHYRIVPEL
                     VYDRSCTVLFGTSTFLGHYARFANPYDFYRLRYVVAGAEKLQESTKQLWQDKFGLRIL
                     EGYGVTECAPVVSINVPMAAKPGTVGRILPGMDARLLSVPGIEEGGRLQLKGPNIMNG
                     YLRVEKPGVLEVPTAENVRGEMERGWYDTGDIVRFDEQGFVQIQGRAKRFAKIAGEMV
                     SLEMVEQLALGVSPDKVHATAIKSDASKGEALVLFTTDNELTRDKLQQYAREHGVPEL
                     AVPRDIRYLKQMPLLGSGKPDFVTLKSWVDEAEQHDE"
     gene            28826..29857
                     /gene="galR"
                     /locus_tag="ACHOSC_RS04055"
                     /old_locus_tag="pgaptmp_000811"
     CDS             28826..29857
                     /gene="galR"
                     /locus_tag="ACHOSC_RS04055"
                     /old_locus_tag="pgaptmp_000811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator GalR"
                     /protein_id="HGU4546801.1"
                     /translation="MATIKDVARLAGVSVATVSRVINNSPKASEASRLAVHSAMESLS
                     YHPNANARALAQQTTETVGLIVGDVSDPFFGAMVKAVEQVAYHTGNFLLIGNGYHNEQ
                     KERQAIEQLIRHRCAALVVHAKMIPDADLASLMKQMPGMVLINRILPGFENRCIALDD
                     RYGAWLATRHLIQQGHTRIGYLCSNHSISDAEDRLQGYYDALAESGIAANDRLVTFGE
                     PDESGGEQAMTELLGRGRNFTAVACYNDSMAAGAMGVLNDNGIDVPGEISLIGFDDVL
                     VSRYVRPRLTTVRYPIVTMATQAAELALALADNRPLPEITNVFSPTLVRRHSVSTPSL
                     EASHHATSD"
     gene            complement(29864..31126)
                     /gene="lysA"
                     /locus_tag="ACHOSC_RS04060"
                     /old_locus_tag="pgaptmp_000812"
     CDS             complement(29864..31126)
                     /gene="lysA"
                     /locus_tag="ACHOSC_RS04060"
                     /old_locus_tag="pgaptmp_000812"
                     /EC_number="4.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008836 - diaminopimelate decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate decarboxylase"
                     /protein_id="HGU4546802.1"
                     /translation="MPHSLFSTDTDLTAENLLRLPAEFGCPVWVYDAQIIRRQIAALK
                     QFDVVRFAQKACSNIHILRLMREQGVKVDSVSLGEIERALAAGYNPQTHPDDIVFTAD
                     VIDQATLERVSELQIPVNAGSVDMLDQLGQVSPGHRVWLRVNPGFGHGHSQKTNTGGE
                     NSKHGIWYTDLPAALDVIQRHHLQLVGIHMHIGSGVDYAHLEQVCGAMVRQVIEFGQD
                     LQAISAGGGLSIPYQQGEEAVDTEHYYGLWNAAREQIARHLGHPVKLEIEPGRFLVAQ
                     SGVLITQVRSVKQMGSRHFVLVDAGFNDLMRPAMYGSYHHISALAADGRSLEHAPTVE
                     TVVAGPLCESGDVFTQQEGGNVETRALPEVKAGDYLVLHDTGAYGASMSSNYNSRPLL
                     PEVLFDNGQARLIRRRQTIEELLALELL"
     gene            31248..32183
                     /gene="lysR"
                     /locus_tag="ACHOSC_RS04065"
                     /old_locus_tag="pgaptmp_000813"
     CDS             31248..32183
                     /gene="lysR"
                     /locus_tag="ACHOSC_RS04065"
                     /old_locus_tag="pgaptmp_000813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator LysR"
                     /protein_id="HGU4546803.1"
                     /translation="MAAVNLRHIEIFHAVMTAGSLTEAAHLLHTSQPTVSRELARFEK
                     VIGLKLFERVRGRLHPTVQGLRLFEEVQRSWYGLDRIVSAAESLREFRQGELSIACLP
                     VFSQSFLPQLLQPFLARYPDVSLNIVPQESPLLEEWLSAQRHDLGLTETLHTPAGTER
                     TELLSLDEVCVLPPGHPLAVKKVLTPDDFQGENYISLSRTDSYRQLLDQLFTEHQVKR
                     RMIVETHSAASVCAMVRAGVGVSVVNPLTALDYAASGLVVRRFSIAVPFTVSLIRPLH
                     RPSSALVQAFSEHLQAGLPKLVTSLDAILSSATTA"
     gene            complement(32170..32862)
                     /gene="ygeA"
                     /locus_tag="ACHOSC_RS04070"
                     /old_locus_tag="pgaptmp_000814"
     CDS             complement(32170..32862)
                     /gene="ygeA"
                     /locus_tag="ACHOSC_RS04070"
                     /old_locus_tag="pgaptmp_000814"
                     /EC_number="5.1.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid racemase"
                     /protein_id="HGU4546804.1"
                     /translation="MKTIGLLGGMSWESTIPYYRLINEGIKQRLGGLHSAQVLLHSVD
                     FHEIEECQRRGEWDKTGDILAEAALGLQRAGAEGIVLCTNTMHKVADAIESRCTLPFL
                     HIADATGRAITGAGMTRVALLGTRYTMEQDFYRGRLTEQFSINCLIPEADERAKINQI
                     IFEELCLGQFTEASRAYYAQVIARLAEQGAQGVIFGCTEIGLLVPEERSVLPVFDTAA
                     IHAEDAVAFMLS"
     gene            complement(32991..34409)
                     /gene="araE"
                     /locus_tag="ACHOSC_RS04075"
                     /old_locus_tag="pgaptmp_000815"
     CDS             complement(32991..34409)
                     /gene="araE"
                     /locus_tag="ACHOSC_RS04075"
                     /old_locus_tag="pgaptmp_000815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arabinose-proton symporter AraE"
                     /protein_id="HGU4546805.1"
                     /translation="MVTINTESALTPRSLRDTRRMNMFVSVAAAVAGLLFGLDIGVIA
                     GALPFITDHFVLTSRLQEWVVSSMMLGAAIGALFNGWLSFRLGRKYSLMAGAILFVLG
                     SIGSAFATSVEMLIAARVVLGIAVGIASYTAPLYLSEMASENVRGKMISMYQLMVTLG
                     IVLAFLSDTAFSYSGNWRAMLGVLALPAVLLIILVVFLPNSPRWLAEKGRHIEAEEVL
                     RMLRDTSEKAREELNEIRESLKLKQGGWALFKINRNVRRAVFLGMLLQAMQQFTGMNI
                     IMYYAPRIFKMAGFTTTEQQMIATLVVGLTFMFATFIAVFTVDKAGRKPALKIGFSVM
                     ALGTLVLGYCLMQFDNGTASSGLSWLSVGMTMMCIAGYAMSAAPVVWILCSEIQPLKC
                     RDFGITCSTTTNWVSNMIIGATFLTLLDSIGAAGTFWLYTALNIAFVGITFWLIPETK
                     NVTLEHIERKLMAGEKLRNIGV"
BASE COUNT         8429 a         9502 c         8558 g         8028 t
ORIGIN      
        1 cgtcgcccca tcggcaaaat acgctttaat ccccgcaaga atagactccg caacttcctg
       61 ctgaaaggtc gccgttttca gtttacgctc ttcctcaacg ttactgataa acgccgtttc
      121 gaccagaatg gagggaatat ctggtgcctt tagtacggca aacccggcct gttcaacctg
      181 atttttatgc agcttgttga ttttaccgag cttattcagt accgctttac caaacttaag
      241 gctgtcagca atggtcagcg actgcaccat atcgaacatg gtgtggtcga catagcggtc
      301 accgcttttg ctcacgccac caatcaagtc cgaggcgttc tgggtttgtg ccagatattt
      361 tgccgcagta ctggttgcac ctttggttga gagcgcaaac acagaggaac cgctcggctg
      421 acgactggta aaggcgtcgg catggataga gacaaacagg tcagcacgct gtttctgggc
      481 ttttgctacg cgcacttgca acggaatgaa gatgtcttca ttgcgcgtca tgtacacctt
      541 catattgccc tctttctcga tcagagagcg caaacggcga gctatttgca ataccacatc
      601 tttttctcgc gttttgtatt tccccaccgc accggagtct tcgccaccgt ggccagggtc
      661 aagcataatg acaatcggac gatcgcgccc tgctttaccc ggttgtggac cactttgtgc
      721 tggcggcacc tgcttttcga ggtcgccttt gttgtaatcc tccagcagcg ccagcagcgg
      781 gtcctgcata tcctgtgcat tggccggata gaggtccatc accagacgct ctttaaaccc
      841 ggcgaccggc gcaagggcaa acagctgcgg ttttacgttt tgctttaatt caaaaaccat
      901 ccgtacggtc tgcgggtcaa attgcccgac gcgcgccgac ttgatgaacg ggtcgtctgc
      961 acgaatttgt gccgccatcc ccttgagtac cgagttcagg tttacatctt cgatatccac
     1021 caccacgcgt tccgggttac tcaacgcgaa ctgcttatat ttcagctgac gatttgattc
     1081 taccgtcaca cgggtgtagc tggacgcagg ccagacgcgc accgccacga cctggctgac
     1141 cgcagccaga ctgacctgac ttacgctcaa tagccacatg gcacccgcgc cttgcagtaa
     1201 acgacgacgg ctgattgcag tgttggatcc tgacatgcct ctcccgagca aaagaaatct
     1261 aagctgtgta acaagtaaac gactaatttg accggaaact ttagcgaaag acgcataatc
     1321 tgtcatctaa taaacggtaa acattctttt tatattcacg gcattactga taaaaaagtc
     1381 gctctcgcat aaaatttaca cttgcaccct gcgaaaaaac agaataaaaa tacactaatt
     1441 tcgaataatc atgcaaaggg gtgtgccgtg gtaaaggaac gtaaaaccga gctggtcgag
     1501 ggattccgcc attcggttcc ctatatcaat acccaccggg gaaaaacgtt tgtcatcatg
     1561 ctcggcggtg aagccattga gcatgagaat ttctccagta tcgttaatga tatcgggttg
     1621 ttgcacagcc tcggcatccg tctggtggtg gtctatggcg cacgtccgca gatcgacgca
     1681 aatctggccg cgcatcacca cgaaccgctg tatcacaaga atatacgtgt gaccgacgcc
     1741 aaaacactgg aactggtgaa gcaggctgcg ggaacattgc aactggacat cactgctcgc
     1801 ctgtcgatga gtctcaataa cacgccgcta cagggcgcgc atatcaacgt cgtcagtggc
     1861 aattttatta ttgcccagcc gctgggcgtc gatgacggcg tggattactg ccatagcggg
     1921 cgtatccggc ggattgatga agacgcgatc catcgtcaac tggacagcgg tgcaatagtg
     1981 ctaatggggc cggtcgctgt ttcagtcact ggcgagagct ttaatctgac ctcggaagag
     2041 attgccactc aactggccat caaactgaaa gctgaaaaga tgattggttt ttgctcttcc
     2101 caaggcgtca ctaatgacga cggtgatatt gtctccgaac ttttccctaa cgaagcgcaa
     2161 gcacgggtag aagcccagga agagaaaggc gattacaact ccggtacggt gcgctttttg
     2221 cgtggcgcag tgaaagcctg ccgcagcggc gtgcgtcgct gtcatttaat cagttatcag
     2281 gaagatggcg cgctgttgca agagttgttc tcacgcgacg gtatcggtac gcagattgtg
     2341 atggaaagcg ccgagcagat tcgtcgcgca acaatcaacg atattggcgg tattctggag
     2401 ttgattcgcc cgctggagca gcaaggtatt ctggtgcgcc gttctcgcga gcagctggag
     2461 atggaaatcg acaaattcac cattattcag cgcgataaca cgactattgc ctgcgccgcg
     2521 ctctatccgt tcccggaaga gaagattggg gaaatggcct gtgtggcagt tcacccggat
     2581 taccgcagtt catcacgggg cgaggttctg ctggaacgca ttgccgctca ggcgaagcag
     2641 agcggcttaa gcaaattatt tgtgctgacc acgcgcagta ttcactggtt ccaggaacgt
     2701 ggatttaccc cagtggatat tgatttactg cccgagagca aaaagcagtt gtacaactac
     2761 cagcgtaaat ccaaagtgtt gatggcggat ttggggtaag cgatgaaaat cgccggatgc
     2821 gacatgcgta acactcgtgc gtcgcatcag gcaattacgt ttattcccgc gaactaaaca
     2881 atgccgccag accactgcgc cgctcagtgc gcgtggcgat tgccgcactt aatatgcgct
     2941 catcggcata cagcgacaga cggcgacgcg cgcgggtcac tgcggtgtaa accagctctc
     3001 gcgttactac cggcgtgcgt tggctcggta aaatcaacgc cgcatggtcg aactccgatc
     3061 cctgcgattt atgtaccgtc atcgcccacg tcgtttcgtg ctctggcagg cggctcggtt
     3121 gcacagactt aatattgccg tccggcatcg caaaccaaac gcgcgtcccc tgcccgcgat
     3181 ccagcgcaat accgatatcg ccattaaaca acccaagcgc gctgtcatta cgggcaatca
     3241 tcaccggtcg accttcgtac caacgagagt gcggatgacg atgaattttg cgcttctgtt
     3301 gcataaactg ctcaattcgc tcattcagtc cagccacgcc aaacggccct tcccgcaggg
     3361 cgcacaaaag ttggtactca ttgaacgcct gaatgattaa atccggctcg gcacgcgctt
     3421 gcagcagatc cagataacgt ccataacccg caagagcttc ctcaagcatc gcaatataat
     3481 cttctccgct ctgtaaaagt cgtttttcga tatcagtaaa atcctgctga aaaacggttt
     3541 tcactgccgt tttatcacca cggttgatcg ccgcagctaa ctgaccaatg ccagaatcgc
     3601 tgccgaaacg atagcttttt tgcagcaggc agagactgtc gcgcaaagat gccgcttctg
     3661 tgccggttcc tgccggaacg tgagtccccg tcaggcggct tagctgcctg gcacgctcgg
     3721 cggtaaagcc cgcgttggca taagcgcaga tatcgcccag cacagcccca gcctcaaccg
     3781 aggccagttg atcacgatcg ccgagaaaga tcactcgcgc atgatctggc aaggcgtcga
     3841 tcagtctcga catcataggc agatcgatca ttgacgcttc atctaccacc agcacatcaa
     3901 gatgcagcgg gttaccggca tgatgacgta aacgctggct acccggctgc gcgcccagca
     3961 atcggtgcaa agtgctggca tcttccggaa tgcgtttctt ttgttcatcg gtcagcggta
     4021 actgtcgcaa agccttgccg agagattcgg ttaagcgcgc ggcagcttta cccgttggtg
     4081 cagccagacg gatacggcag cgttcgccgt cggccatttg aattaacgct gccagcaact
     4141 tcgccacggt ggtcgtttta ccggtgcctg ggccgccgga aaccaccgag atccgccgcg
     4201 tcagcgccac tgccgctgca actttttgcc agttaatttc atcgcttact ggaaaaagtt
     4261 tgtccagggt ttgcgccagt agagcttcat caacctcaat ggcatgattc acctcattga
     4321 agaagcgcgc taccgtccgc tcgttacacc acatgcgatt caaataaaga cgatcgccac
     4381 agaggaccat cggcgtgggt tcatctcccc tgctgaccgc ttgagaagcc agcaagcatt
     4441 cttcccaatt ttgtagctca ccgatttcac tgacacaggt cgccaacagc ggatgcgacg
     4501 cctcgttatt ttccagtcgt gaaagcggca aacagacgtg tccctctccg gcatcatgac
     4561 ttaacagtgc cgccgcgagg gtgacggcag gatgttcatc tcccgccacg gtcagggcaa
     4621 attgcacatc cagcgggcgt agctgtttgt gctccacagc ttccagtaat tgcttttgca
     4681 atttcattac gcctcctcca gggtcatacc ggcaaacatc tcatccatca gggcaatcaa
     4741 cccggcgttg ggtcgggttg tgtaaatccc ttgttgcgga tgttctttat caacgccacg
     4801 caggaacaga tagataacgc cgccaaagtg acgatcatag tcgtaatcag caatgcgatg
     4861 gcgcagataa cgatgcagcg ccagggtata aagctgatat tgcagatcat agcggtgtgc
     4921 ctgcatcgct gctgccatag cctgttgggt gtaagccgaa ctgtcttcac ccagccagtt
     4981 ggatttatag tcgagcaggt aataacgccc ttcgtggcgg aataccaggt cgataaagcc
     5041 ttttaacatg ccgcgtacct gcatgaactc cagcggcggg cagccagcgg atagcgggtc
     5101 aaactggcga attagtgcat caagctggct ggcgatgagc ggttcgctaa tcggcaggta
     5161 aaactccatc tccacctgtt tatcgcggtc ggaaagctga ctcaggctaa cacccgtttc
     5221 attgagaggt gcctggagga cagccgtgat ccactcggtc aacaccggtt cccactgcga
     5281 ttcaaagccg ccgagttcca gtttttcctg tacccagttc gggtcaaccg gctgggtaaa
     5341 atcgaggtct tcaaacaaac tgtgcaagaa tgtccccggt gacgcaccgc gcgggaactg
     5401 atgcggtgtt aacgtcggtt cttcaacgac gctgaccacg cctgcggcat caacatccag
     5461 ccgtggcatc agatcctgag cgataccgtg accacgctgc tgcaagccgg agtagctggt
     5521 gacacgccag ttatcgccgg gcaatcgttg tagcgtcctc gcattcagtt ctgcagttaa
     5581 agcatcatta acctgccagg gctggttatc accagtttgt gccgtttgcc aggcaatatc
     5641 atcatcgcat aacgcttcaa tacaggtgcg aagccctgcc gcatcttgcg gttccccttt
     5701 ttgcagcaaa cgcccgagcg cactttggtg gacgtcggtg tcaccttttt tatcgccacg
     5761 acggcgcacc agcggtgcaa cgccgagact gcaatgccaa accgaacgcg tcagcgccac
     5821 gtaaagcaaa cgcagatctt ccgccagacg ttcgacctcc gcaagctcga cgctttctgg
     5881 cgcagcgtta agatccagaa cagcctcaaa cgagtggcga tcgtgataaa acgcctgatc
     5941 ctggacgcgg aaattggtaa taaatggcaa ccagaccagc ggatattcca gccctttcga
     6001 tttatggatc gtgacaatct gcaccagatg tttatcactt tcgaggcgca tttgttggct
     6061 ggaggcatta ctgtctggct cgaggatatg ttgcgataac cagcgtacca gcgcatgttc
     6121 actttccagc tgcgttccgg cttcttgtag cagttcgctg atatgcaaga tatcggtaag
     6181 acgccgctca ccgcctgccg ttgccagcaa gttttctgca atgttacgcg ccgacatcag
     6241 cgcccgcagc atcggcataa cgccacgttt gcgccagatt tgccgataac catcgaactc
     6301 ttcaactacc gcatcccacg catgttcgtc attattcagc gtttcgatat ccagcgcgtt
     6361 cagccccatc attgacgttg ccagcgcact gcgcagggtg ttctcacgtt cgggcgtcat
     6421 caccgcctgc aacaaccaaa gcatttcctg cgcttccaga gtttcaaaaa cactgtcgcg
     6481 gttcgaaagg taaacggaag ggatttccag caacgttaag gcatcgcgca cctgggcggc
     6541 ctcctggcgg ctgcgcacca gcacactgat gtccgaagca cgcaccggac gcgcgtcgtc
     6601 gccgttcatc agcaacgctt cgccccgctg tccggcttgt agccagtcgc ggatttgcgc
     6661 agcacatacc tgcgccatgg tactttgata atcgccaacg ccgcagcttt cgccttccat
     6721 cagccacatt ttcatcgcag gctgcgtttc acctttaaac acaaaacgta acgcctgatt
     6781 tttcccggct gatttcactg gaataaacgg tatttcgcga aacatgaacg cgtcatcagt
     6841 ctggctgaaa agcttgttca cactgttcac cattccaggt gcggagcgcc agttggtgtc
     6901 taaagtgtag tgggcgtgaa cttcactacg cgccttcata taagtgaaga tatccgcacc
     6961 ccggaatgca tatatggcct gcttcgggtc gccaatcagc aacaatgcgg tttccggctg
     7021 atggtgccag atacggcgaa aaattcggta ctgctggggg tcggtatcct gaaattcatc
     7081 gatcattgcc accgggaatc gcgtacggat cgccgctgcc aacacctcac cgctttcgct
     7141 acgcagcgcg gaatcgagcc gacttaacat gtcatcaaaa cccaattcgc cacggcggcg
     7201 tttttcacgc gctactgttt cgcggatctc agccaatgcg cgggtgatca ccagatcgcg
     7261 gatcgacaat ggttctgcaa gcagttgatc gatcgcctca aacagtggat gtcgcggggt
     7321 ttcccccccg gccttcgtgc gatcttctaa gaaacgctgg gagaattttt ccagcgactc
     7381 cggcaactga taactgtttg tctcttcttc tgcccaggcg ctgatctttt cgatccattt
     7441 agcctgattg ctacggttaa acttgcgtcg atcaatacca gaagattcga tcagcgcatc
     7501 aagttcaccc actgcgtcgc gccactgctg ttttaccgta tcaatacgcg ccacaatttg
     7561 cgcgtggcgg gaagccagcg tttcatcatc gggcggcggt gctttgataa ccggtgcttc
     7621 gccttgcaga taacgattaa tatcgcgcag caacgcctgc ggccctttcc aggtttcaaa
     7681 gaccaccagg gcaatttcac gcggcagcgg gtagcagtgg cgacgccaga aatcggcgca
     7741 ggcctggtag cgtagcagag actcatcttc aatcagctgc tgctcaaaca gcataccgga
     7801 ttcaaaggca ttcaggttga gcatgcgctg gcaaaaaccg tgaatagtaa agactgccgc
     7861 ttcatccatc tgccgttcgg ctaacaacaa ccactgcgcg gcttgcgctt tatcgtcgat
     7921 ctcttccagc aggcgttcgt acagtggatt gtcggtggtt tcacgcagac aggcgatgcg
     7981 caactcgtgg atattgctac ggatacgacc acgcaattct gccgtggcag cctcggtaaa
     8041 ggtgaccacc agcagttctt caacggtcag cgggcgggga aaggcggcgg aaccgcctag
     8101 tccaagtaac aggcgcaaat agagcgccgc aatcgtaaag gttttgcctg tgccggcaga
     8161 ggcttcaatc aggcgctcgc cctgtaaggg caagcgcaaa ggatctagtg tctcggcgac
     8221 atcactcatt cttttcactc atcaggggca ttgtttgctg caacgcgctg acgttctccc
     8281 acactttcca gccttcagga tgcacatatt cggctttccc gttctggctg ccggaaatct
     8341 gcgacagaat agccatgcct tgcggctcga ccaccgccag atggaagaaa tcagcaagtt
     8401 tttgcggcgt cagcagtttt atctgggcca cgattttatc acgcgaatcg aagcgcatat
     8461 tgccgcgatc gaaatcttta cttaacttcg atgcttcttc gccgagcgtt tgcggtgcct
     8521 gcagcatctg ggtaattacc gcctgctgga tttgcgcaaa ctcttctggc ttcatcgccc
     8581 gcaattttgc ctctgcggtt gggaaaaacg ccttgtaacg ctcccacaag aaggaaggct
     8641 gtttatcatt gctttgcaaa aggaagccca tgccccactg acgccccacg ctcattggaa
     8701 atgcaaacac ggcatagccc aattgttctt cggtacgcaa ctgattgtag aaccacggct
     8761 gtacgatctg ccccaacaga gagctatagg ctgagctggt gtattcatcg tagccagtcg
     8821 gtacaaatac cgctgccagt gcggagtcgg tgctgttacc agctttttca aagatgacgg
     8881 attgtttttt atcgaccacg acatctttgt tacgacacca ctctgaacca tcagcgccca
     8941 actgtttttg cacatcgcgt gccagcgttg ttgcctggtc ctcggtcatg ttgccgataa
     9001 ccataaactc tggtcgagcc cctgatttta aggcgtcgcg ataggccagc acctctttca
     9061 acgtaatgga gggcaaaatt ttgcgccgtt catctcgcga gaagtacggc acttgcgaga
     9121 gcatctgcgc gggcataatc gcctgctcga acgctttgcc cttttctgcg gaatccatca
     9181 tctggttata ccaggacttc gcctgctcaa gctgatcttc cgtggcggta tagctaaagt
     9241 agccttccag caatgcctgg aacagctgcg gcagatgctg ggtgtaacca ttagcattaa
     9301 ccataaggcc gttgttagcg ttggtggaaa aacttatgcc accaaccgac gcctggttgc
     9361 ttaactgatc aagcgccagc cctgcgagat aatcattgag cgcaaacatc acctgattgc
     9421 gggcgctgtc catggctttc ggattacgca aaatcaggct gacatcggct tttggctcgc
     9481 tggcaaaata acggcttggc gcatacacca cgcgcagatt cgactcatca acaatcagct
     9541 ctggatggtc atatttcttg tctgacttaa tcagcgagaa atcgtcagga atataagggt
     9601 taagctctgg caaagagagc gcaatgtcgg cggctttttt ctgccagtcg gcgaaagttt
     9661 gtgcgctgat tttatcgacc tgatacggcg catcgacaaa gtaagccgtt ttgttgtgcg
     9721 gctctttcgg gctgatatac cagatacgcg cattctgcgg cgtcatcatc gccagacgtt
     9781 cctttactgc tttagcatcg taccgatcgg caatattgac tgcatccagc gtatgctcaa
     9841 caggaacgcg aatcatggta tctgccagcc attcgacgta atccatatca cgggtgattg
     9901 acggataacg gaagtcgata tccagcacat tcgccagttc atcgaagtat tgtttatcga
     9961 tccctttttc acgtaacaaa ttgaggtagc taaaaatagc cgccacaacc tgatcgcgat
    10021 tcgccagacc tttatcggtt aaagacgcag agatcgctaa tacgccgctg ttgccgttga
    10081 cgataggatc ggagttggcg ctaatgccct caactaatcc ctgcttttgc agccagtcgg
    10141 aaagtgtacc tgggctgcga ttaccaatca gataggtaat caattcatcg gttttgctac
    10201 ggaatttcgc cgagttgtta tcaatacgaa actctacgcg caacacttta cgcggcagcg
    10261 ccgggacgta atgaatgata atgccctttt gcgcgtcggt gactaccggc acggtgattt
    10321 ccggtttttt gctctctttg ttcggcacgc gaccaaaggt gtccgccgcc atttttgcca
    10381 actccggcag cggtttattg ctgtaaataa ccgccttcat cagattggcg gaatagtact
    10441 tctcgtggaa atctttcagc gcctgctgca ccggattacc aggtttgtcg cttaaagttt
    10501 cgaggttacc accagaaaac tttgaaccgg ggtgtgccgg gttaatggtt tctgcgctga
    10561 cctgtgccat gcgcatcccg tcacgcgtac gcgccatggt taattcagcg ttcactgcat
    10621 tacgctcacg ttcggcgtat ttcttgtcga gcaagggttc tgcaatagca tcagccaggc
    10681 ggtctaccgc accaggcaag gcgtcgttct caacttccag atagaaagcc gtgcgatacg
    10741 gcgcagtgct ggcattgtga ctaccgccgt gcattttgag atattcggcc agactgtcag
    10801 cctgcgggta ctttttcgac cccatcagac tcatatgttc aaggtaatgt gccagcccct
    10861 ggtacgcctc gggatcttcc agcgacccaa cgggcaccac cagcgccgag agcgatttaa
    10921 ctgcctgcgg atcagaaacc agcaagacca ccataccgtt atccagacgt atagcctgat
    10981 actggcggtt atctttatca cttttacgga tggtttcctg aatcggctgc catcccgttt
    11041 ctgcctgact taagggtgcc caaagggcaa ctaacaacaa taatgctttg aaccaggtgc
    11101 tgcggggcat tcacggacct cataatcaac ttaattttct gtccagattc aacacgttaa
    11161 cgcaaatcat ctgccagaat ttaatcttgt gctgcacgag tcagcctatg tttatataac
    11221 catcagtccg tgactggtgc gcatcataaa gtaagcggat agattgcgca atttttatac
    11281 agcactcatg actgattaaa gcgaaacagc ggtaacagga aacgttgcga ctgttcaacg
    11341 atggcctcca ttgtctctgg tgttaattgc cgccagagcc tttgatacca gatatcatca
    11401 ccttcgccac gcaccatcat gttgccttcg taagcctgaa ggaatttcgt acgggctttt
    11461 tgcaacgtgg aatcgtcatc cagcatggca tcgttttgcg cgtcataaca ggtttttagc
    11521 cacgcgccgc cactttcagg taacaccagc aatggcgcgg acatcccttc acgatacccc
    11581 tcaatcagtt gtgagaggta atgcaaagcc tgttcggctg caagcggcgg aaaacgccac
    11641 tcgccgtctt tgcgtagaaa aaggcgactt tcaccattac caccgctggc acagtagaca
    11701 aggtgttcca gccaaagttg cattccctgc gccacactta ataaagaggg acgccagcgc
    11761 aacaggccat ccggctgcac ctgcggcaac cagccagtta tctgcacacc gttgcaggcg
    11821 agatcaattt ccatactctg ccccggctgg cgacaggcaa tgactctgtc ggcaagctgc
    11881 tgcatctcct ggcactgtgt ttcccagaaa atttcaccaa aagcgccata cggtaaatcc
    11941 cctgccgccc ggaagcggcg gaacaagcgt tcggcatcat cctgctcaac cagtgcattc
    12001 aataactgct gattgatttg ataacggcta agcccttcca gaataaatgg ctcggtgtct
    12061 gggatttcgc tgtcttcagt acggaagttc acctgcaaac gcatctggaa gaacgcccgc
    12121 accggatgtg cccagaatcg ttgtagcgtt tccagcggca cggtttccgg taaggtaaac
    12181 ggcagcggct gaacaaattc agaatgtgct ttaccagcct ggctggccgc aggtagccat
    12241 tcacgagcat agctttgtcg ttcgcctggc tggtagtttt gcggatcaaa cggcatccgg
    12301 gtatggaggc aagtaagatg cgcttttacc cttgcctcgc tttcatcaca gttgagcgct
    12361 tcatcgtccg gtagataatg actttgcccg atgtagtcga tcagttcctg caccagtacc
    12421 gacgggaaac gttcactgtt atcctgaatg gaacgcccga tatagctgat atagagtttt
    12481 tgctgcgcgg aaattaacgc ttccaggaac aggtagcggt cgtcatcgcg acggctacgg
    12541 tcgccacgct tcggtttctg gctcatcagg tcaaagccca atggcgcaag ctgacgtgga
    12601 taaacgccgt cgttcattcc cagcaggcaa accactttga acggaattga acgcattggc
    12661 atcagagtac aaatgttaac cggtccggcg agaaaacgct ggctgatacg ttcttgatcc
    12721 agacgctgcg ccagttcatc acgcaatagt gacagcggca ccgcgtcgcc atactgcgca
    12781 cctaaacctt cggcgataat cgcctgccat tgttgttcga tcagcgtcat cgccgcttcg
    12841 gtttccgcat ccggcaggaa gaaggcgttg agcatatcgc gacaaaccgg caaccactct
    12901 tctagcggac gctcctgtgc cagcccgcga cgccagatgt ttagctgcat tagcagtgaa
    12961 gccagatgcc ccaccagttc tgcaattaag ccgctcgatt catcataagg tagaaccgat
    13021 tgccactcgc cctgcgcgct ctccatcgcg tagcccagca acatacgcgt caggccaaat
    13081 cgccaggtgt gttgtccggt ggcggggagt tccagctcgc gaacgttgtc gtcatctatc
    13141 ccccaacgaa tgccggattc gttgacccac tggcgtaaat aacgcagccc ttcttcggtg
    13201 atgtcaaacc gcgccgccag caccggcaca tccagtaatg ccagcacgtc ttccgacaca
    13261 aagcggctgt ctggcaacga taacaggctg ataaacgctt ccagtaccgg atgcgactgc
    13321 cgcgcccgac ggtcggaaat ggcgtaaggc aggtaacgat ccgcaggtgc gctaccaaac
    13381 acagcctgaa taaacggact gtagctgtcg atatcagcca ccatcacgat gatgtcgcgc
    13441 ggagtaagtg tcgggtcttc ttccagcatc gccagcaggc gatcgtgcaa aacttcaact
    13501 tcacgctgcg ggctatggca aacgtggaag gtgatactgc tatccagtgg atcaagcggg
    13561 cgtttgttat cgctacggga aaactcttcg atgttcacac cagcaacggc acggttttcc
    13621 agttccagaa tgtcagactg aatgttatgc agcaggttat ctggcgtgac atcgacaaag
    13681 gcgtccagct cctggctgct ctccaggtca gaaaggagat aaatgtagtc gcgcccaagc
    13741 ttaccccatg aagccagcag cgggttgccg acatcctgtt caccatcgct gttaaagagc
    13801 tgcccggcat tttcgctatc acgaaatagc ggcaattcgc gatcttcaac actgtgtcga
    13861 cgctgacggg tcagcagttt cgccagataa gcaggatctt taatatcgcc ccagtaataa
    13921 cggcaggggt tggtaaacag gagatggatt tcaatatgtt tacccagcgc ctggagcgcc
    13981 tggagataaa caggcggtaa cgcggaaata ccgcatataa agacgcgcga aggtaacccc
    14041 ggcgggcagg tcgtcgcgga ctccagcgtt tcgataaagc gctgatagag attggcgcgg
    14101 tgccaacgcg gttgtccgag ttcatgggta tattccacca gcgccttcca caacggcgct
    14161 tgccaggcct gtgcttctcc cagcccttca accaaatgtc ctgtttccca ctgtgccagc
    14221 cactccgggc gatagaccag atactggtca aacaggtccg ccgcttttga ggaaagctgg
    14281 aacagttttc gcttgtcgct atcgtcagcc agataatgcc gcaacagggt aaagtcttcg
    14341 cgatccagca attgcggcag cagagtcatc agtttccagc tcatgctctg tttgttaaag
    14401 gcgctctctt tggggatctc cggtaacacc cggacgaaca tatcccagat aaagctcgct
    14461 ggcagcggaa aatcaatgtt tgccgcaata ccaaactttt gcgacagggt catttgcagc
    14521 cactgcgcca taccggtact ttgcaccaga atcatctctg gttcgaaagg atcgtccagc
    14581 cgttcgcgtt cgacaataaa ctccatcaac gcttccagca cgtccagacg attggaatgg
    14641 tagaccctta acatagcggc tcctgactac tgacgattcg ggcaatgcag gcgggtcatc
    14701 tcgccttctc tgcccccggg tgaaactagc gtaacgctga tgctgacaca tcccgcctgc
    14761 gatgtctgca ttcggttgac ctgccagttg gcaggtggcg aaatcgcgcg cagttgcgtt
    14821 tgctgccaac catgccgcca gagctgttgg tactggtttc tgctggcgag actgttcatt
    14881 aatgttcgct ggatacccga taacgcagtg acaatcatca ccatcagcac catcgcaacc
    14941 attacctccg gcaggctaaa gccttgttga ttcttcaggg aagctgacat aacgccctct
    15001 ctttcagcgg acaaaaatcg ctccagccgc gtggcgaaaa aacaatgttt ccatcaatga
    15061 cttcgcctgt tcgccacaac gaaacgcctt cataaccggc aaccaataag gcttcattat
    15121 ctgccagtaa acgcaaacaa acccgggcat cggttccagc gtactgcgag cactgaactg
    15181 cgggctgcgt ctgccaggag tgcattttcc cccacgccag cgccgactga acgatggcct
    15241 ggcggcgcaa tgactgactt tccatgctca cgcgagaggc aaaactgcga tcctgctgac
    15301 tcattccttg caatagcaag ctacccaaaa ccagcagcat caggaccaga gccagtgacg
    15361 aaacaccttt ttcgcggttc acaggttgaa tcctgtcacg ctatagctgg catccaccac
    15421 ggtttgcggt tcagacttac tggcagcacg catattaacc gtcaacaccg gcgagaagcc
    15481 gctgacatcc tgacgtacga cctgaaaagt gtcgataatg atggcatccg gattagtgac
    15541 tttatcccag cccttacctt cacaggatgt cgcaccgcgt agcgtttcca gcacatgctc
    15601 cttcagacga aatccaatct ggtcagactc ttttaccggt tcgcgatccc agataccgtt
    15661 actgttcgca tcccactgca caatgataca gtcaccctgt ccgacaattt ccagcccttc
    15721 gccggtacag ctgccatgac aataacccgc cctctggaga tgcttcgcga cggtaaatac
    15781 ccgcagccag atttcatctt ccagcgccag cttacgggtg ctcgttaaac tttcacgctg
    15841 taacgcaggc agaaagcgtg cagcccccag caacaatacg ctactgatcg ccatagcaat
    15901 caacacttcc agcagagaaa aaccttgctc ttttacaggc atccttctgt ttctccttgc
    15961 tgacaaagcc ggagtcttcc ccacggcgaa accaccagcc accactcgcc cgttgagttt
    16021 ttgaagcgaa tatgcccggc ccatgcggta ttgcgcaggc caaagaaagc aagcgaaggt
    16081 gtcaggtcgc tcatttcgac ttcgggccag cgtggcacaa agaccaatgg tgaactgcca
    16141 tgacaggtat tggccccagc agcggaactc acaaggcacc ataacgtccc ctccctgata
    16201 acgctgatac tgtggtcgcg gttatgccag ttggcatctt cacgtaaata gagcaaatag
    16261 tcccgcgcct ggctggcggt ttgccatagc cgttgcgact gctgccagta ttgccagcca
    16321 tagagtccac ttgcgcttag catgaccaaa atcagcatcg cgaccagcgt ttcaatcagc
    16381 gtataaccac gttgtgtttt catgccggca gtatggagcg aggagaaaaa aagacgaggg
    16441 ccagtttcta tttcttcggc gcatcttccg gactatttac gccgttgcag gacgttgcaa
    16501 aatttcggga aggcgtctcg aagaatttaa cggagggtaa aaaaaccgac gcacactggc
    16561 gtcggctctg gcaggatgtt tcgtaattag atagccaccg gcgctttaat gcccggatgt
    16621 ggatcgtagc cttcaatctc aaagtcttcg aaacggtagt cgaagatgga ttcgggttta
    16681 cgtttgataa tcaacttcgg cagcggacgc ggttcgcggc ttaattgcag atgagtttga
    16741 tccatatgat tgctgtacag gtgcgtgtcg ccccccgtcc agacaaaatc gcccacttcc
    16801 agatcacact gctgcgccat catatgcacc agtaacgcgt agctggcaat gttaaacggc
    16861 aggccgagga aaacgtcaca ggagcgctga taaagctggc aagagagttt gccgtctgcc
    16921 acatagaact ggaagaacgc atggcacggt gccagcgcca ttttatccag ttcgcctacg
    16981 ttccacgctg aaacaataat gcggcgcgaa tccgggtcgt ttttcagctg gttcagtacc
    17041 gtagtgatct ggtcaatatg acgaccatcc ggcgttggcc aggcgcgcca ctgtttacca
    17101 tacactggcc cgaggtcgcc gttttcatcg gcccattcgt cccagatggt gacattgttt
    17161 tcgtgtagat aagcaatgtt agtgtcgccc tgcagaaacc acagcagttc atggatgatg
    17221 gaacgcaggt ggcaacgttt agttgtcacc agcgggaatc catcctgcag gttaaaacgc
    17281 atctgatgac caaaaatgga aagcgttccg gttccggtac ggtcgttttt ctgtgtgcct
    17341 tcgtcgagca ctttttgcat cagttctaaa tactgtttca tggttcctca ggaaacgtgt
    17401 tgctgtgggc tgcgacgata tgcccagacc atcatgatca cacccgcgac aatcatcggg
    17461 atggaaagaa tttgtcccat gctgatgtac tgcacccagg caccagtaaa ctgcgcgtcg
    17521 ggctggcgga aaaactcaac aatgatgcga aacgcgccgt aaccaatcag gaacaaacct
    17581 gagacagctc ccattgggcg cggtttacga atatacaggt tgaggataat aaacagcact
    17641 acaccttcca gcaacagctc gtaaagctgt gatgggtggc gcggcagcac accgtaagta
    17701 tcgaaaatgg attgccactg cgggttggtt tgcagcagca aaatatcttc tgtacgggag
    17761 ccagggaaca gcatggcaaa cgggaagttc gggtcaacgc ggccccacaa ttcaccgtta
    17821 ataaagttgc ccagacgccc ggcaccaaga ccaaacggaa tgagtggtgc gataaaatca
    17881 gagacctgga agaaggaacg tttagtacgg cgggcgaaga taatcatcac cacgataacg
    17941 ccaatcaggc cgccgtggaa ggacatgccg ccgtcccaga cacggaacag atacagcgga
    18001 tcggccataa actgcgggaa gttgtagaac agaacataac caatacgtcc accgaggaag
    18061 acgccgagga agcccgcata gagtaagttt tcaacttcat ttttggtcca gccactgccc
    18121 ggacgattcg cccgtcgtgt tgccagccac attgcaaaaa tgaaacccac cagatacatc
    18181 aggccgtacc agtgaagcgc cacgggtcct attgagaaaa tgaccggatc aaactccgga
    18241 aaatgcagat agctactggt catctgtcac cacaagttct tgttatttcg ctgaaagaga
    18301 acagcgattg aaatgcgcgc cgcaggtttc aggcgctcca aaggtgcgaa taatagcaca
    18361 aggggacctg gctggttgcc ggataccgtt aaaagatatg tatatgatcc gcgctataac
    18421 cctccgcgaa tcagcccgcc catgccacga cgctccataa aggctgcaac ctgatggcga
    18481 acttcggtcg ccagttgcgc ttccagacta cgctgcgcaa gattttctgc ttcggcataa
    18541 tcaatgcgcc gcagcaggta ttttacccgc gctacagaac gtccgttcat agacagatgg
    18601 tgatacccaa gcccaatgag gattgccacg cacatgggat cgcccgccat ttcaccgcac
    18661 aaacggagat cgattccatg tatttccgct tcccgggcga tcatcgccag agctcgtaac
    18721 attgcaggat gaagactgtc ataaatgttc gccacccggg tattgttgcg atcaacggcc
    18781 agaatgtatt gagtcagatc gttggtgcca acagagatga aatcgacccg ctttgccaga
    18841 tgcggcagca taaataccat tgacggcact tccagcatga tgccgatacg tggtttggga
    18901 atttcgtaac cgatcatctc ctcgacttca cgtccggcac gttcaatcag gcggcgtgct
    18961 tcatcaactt catcgaggct tgtgaccatc ggcaacagaa tattcaggtt gcccgtagcg
    19021 gcattagcac gcagcatcgc ccgcacctgg atcaagaaga tctccggctg atcgagcgta
    19081 atgcgaatcc cacgccaacc caggcatgga ttctcttcgc tgatcggcat gtaaggcagc
    19141 tgcttatctg ctccgacatc cagcgtacgc aaggtgacgg gtttatcatt gaacatttgc
    19201 agcatcccct gatactgcgc cacctgttct tcttccgacg ggaaaccact ttgcagcatg
    19261 aatgggattt cagtgcgata aagaccgatg ccatcaatac ggctgcccag tttttcttca
    19321 tgttccgggc ttaaaccagc attgagcatg acttttatac gctcaccgct ttttaactgc
    19381 gcgggtaaat tgacgtcatc ttccgccaga cggctaagct caatctcttc actaattagc
    19441 cgctgatatt cttgcagcag taccggctcc ggatcgacca gcaattcacc gcgatagcca
    19501 tcaacgatca gcgtcctacg atgcagcacc gaaggctgaa tatccgcgcc catcacggta
    19561 gggatcccca gcgcacgtac catgatcgca gcatgggagt tggctgcgcc atctcgcacg
    19621 acaacaccga ctaagcgatc ctggggcagc tcagcaagag ttgtcgctga cagttcatct
    19681 gccaccagaa tgaaacgttc cggccaggcg ttcggccctt gattagcgtc atcaagatga
    19741 aacagcaatc gctgacccag cgcacgtaaa tcgccagccc gctctttgag atagttatcg
    19801 cttagcgcgg caaactgttc ggcaaatttt tcaatgaccg tttttaccgc ccactctgcc
    19861 accgagcctt tatcaacctc ggcaaacaat tcgcgacgca gccgggtgtc cgaaagtagg
    19921 tgcgagtaaa gatcgaaaat agccgccgtt tctttttgtg ctccggcggc aaagcgtttg
    19981 ctgtagcggc gaaactcgtt tgccgcttct tccagcgccc cggtcagtcg ttcgcgttcc
    20041 agagccggat ccagcgttga tgcctgatac acctgttcca ttaaaggtaa cgtggcatcc
    20101 tgccagcctt cggcaatcgc cacaccaggt gctgccggta atgcgcggat tcgcgtctgg
    20161 cgatattgcc caaacagggc agtcaactgc gactgggaaa gaatagctgc catctgggtg
    20221 gcaagcgtca ccaggaagga ttcttcactt tcgtcatact ggcgcaactc tcgttgctgt
    20281 accaccagta caccaagcaa ctggcgacgt tgaataattg gtacgcctaa aaacgcgcgg
    20341 aaacgttctt cttttacgga ggggatgtat ttgaagctgg ggtgcttttg cgcatctgca
    20401 aggtttatcg gttccgccag cctgccaacc aggccgacga tcccttcatc aaacgcgagc
    20461 gttacagtgc gaccgcgtgg ttttttcagc ccccgggtcg ccatcaggta gtaacaacgt
    20521 cgatcatgat cggccaggta gaccgaacag acctcggtat ccatcgcaag acagatgtcg
    20581 gtaaccagaa tatttaacgc ctcattcagg cgtggtgcgc tggctacctt ttcgactatt
    20641 tcgcgcaggc gagtgagcat aattggcgtg acttaacctc ttttacgtcg ataagcagat
    20701 gcgttttgtg gttttggcgt attttcctgc agtgacatca ccacactcgc gaactctttc
    20761 attaccctac ggtagacatc acgtttaaat gacaccacct gtctgaccgg ataccagtaa
    20821 cttacccatc gccagccgtc aaactctggt gtactgctgg tttgcatatt gatttctgca
    20881 tcgccgctta ccagctgcaa gagaaaccat ttttgttttt ggccgataca aaccggcttc
    20941 gtgtcccaac gcaccaaacg tttcggtaat ttgtagcgca accagttacg cgttgaagca
    21001 aggattcgaa cgtctttgcg gcttaatcct acttcttcaa acaattcacg gtacatcgcc
    21061 tgctctgcgg attctccggg gttgattccg ccttgcggaa attgccagga gtgctgacca
    21121 aatcgccggg cccacattac ctgcccctgg cgattacaaa tcacgatacc tacgtttggg
    21181 cggtagccat cgtcatcaat caccggacta cctcaaaata aagctttata tacgaatgat
    21241 tgtttcatac tccaggaaga cggtaaacca ctctctgcag ggcattacac actaataaca
    21301 attgaataac tcacagttat gtgcagagtt ataaacagag gaaggggtgg atagccgttt
    21361 ttattcacct tttctgtgga tagagttgtg aagaactacg gaattactac gggaaaaccc
    21421 ggagcattct gaataagccc gatatagaca ttttaaaata ttcattatta gtcaatgcat
    21481 tacatgtaat ttgagtaatg aaaaagagta attcgtgacc cagctcacac ctctcattta
    21541 cgggttggcg aaagatcaac caatgccgta tttatccaca gaatgtgcca ctaagttaag
    21601 cactgaacca ctaaaaactg gagtttcgtc gcacgtcaag gctgtaaatg gaaacagtag
    21661 tggaggtttt tcacagttat cccagctttc tgtggataac atggtgtaag atcctgttta
    21721 ttttcagtga ccagatttgg aaaacctatt gcagtgttgc gcaactcgat taccggcagc
    21781 ctaaaacgca atataaatca gagaattgaa caacacatat ggaaaaagtt acactgcgaa
    21841 tattcggcac ataattgctg tttgtttttt aaccaaggta tcatgacatg tcccaacctc
    21901 gcccactgct ctctcctccc gaaactgaag aacagttgtt agcgcaagca cagcaacttt
    21961 ctggttatac attgggagaa ctggcggcac ttgccgggct ggttacgcca gagaatttaa
    22021 aacgcgataa gggctggatt ggcgtgttac tggagatctg gctaggtgcc agcgcaggga
    22081 gtaaacctga gcaagatttt gctgctctgg gcgtggaact taaaactatc cctgtggata
    22141 gtcttggtcg tccgctggaa acaacattcg tttgtgttgc cccgttaacg ggtaatagcg
    22201 gggtgacctg ggaaaccagc catgtgcgcc acaagctcaa acgcgtactg tggataccgg
    22261 ttgaaggcga gcgcagcatc ccgctggcgc agcgtcgcgt aggatcaccg ttgctgtgga
    22321 gcccgaatga agaggaagac cgacagctac gcgaagactg ggaagaatta atggatatga
    22381 ttgttctcgg tcaggttgag cggatcaccg ctcgtcacgg ggaatattta cagataagac
    22441 cgaaagcagc gaatgcgaaa gcgcttaccg aagccattgg tgcccggggc gaacggattc
    22501 tgacgctgcc acgcggcttt tatttgaaga agaatttcac cagtgcacta ctggcccgtc
    22561 attttctgat ccagtagcca tcgctttgac ctgccgcttt ccgggcatat aattaccgct
    22621 tcattttttt ggcagggctt tttagatgtt atttgcatgg ataaccgatc ctaacgcctg
    22681 gcttgcgctc ggtacgctga cgctgctgga gatcgttctt gggatcgaca atattatttt
    22741 cctttctctg gtggtggcaa agcttcccac agcacaacgt gctcatgcgc gccgtctggg
    22801 gttggcggga gccatggtta tgcgtctggc gctgctggca tcaatcgcct gggttacgcg
    22861 cctgacaaat ccgcttttta caatattcag tcaggaaatt tctgcccgtg atttgattct
    22921 gcttctgggt ggcttgttcc ttatctggaa agccagcaag gaaatccacg aatccattga
    22981 aggtgaagaa gaagggctga aaacacgcgt ttcatcattc ctcggcgcta tcgtgcagat
    23041 tatgctgctg gatattatct ttagcctcga ctcggtgatt acggctgtgg gtctgtcaga
    23101 tcacctgttt attatgatgg cagccgtggt gattgccgta ggcgtgatga tgttcgccgc
    23161 gcgctcgatt ggtgattttg tcgaacgcca tccttcggta aaaatgctgg cgctctcttt
    23221 cctgattctg gtgggcttta ccctgattct ggaaagtttc gacatccacg taccgaaagg
    23281 ttacatctac ttcgcgatgt tcttctctat tgcggttgaa agcctcaact tgattcgcaa
    23341 caaaaagaat ccgctctgat actccgtacg ctctcctgcg ggagggcgta ctcactcctt
    23401 ttaccctcac agtttaagat tttcctgctt tcaagatata taacgtcggt ttataaacag
    23461 actattatca taggtaggcc aaatgaggat aaccggatga aaaaatgggc agtaataatt
    23521 tctgcagtcg gactggcgtt tgctgtttcc gggtgttcca gtgattacgt catggcgacc
    23581 aaagatggcc gtatgatttt gaccgatgga aaacctgaaa ttgatgatga taccgggctg
    23641 gtgagttatc acgatcagca aggtaacgcg atgcaaatta accgtgatga tgtttcgcaa
    23701 attattgaac gttaacaaat aaggtcagca tccggctggc cttaagattt ttctctttcc
    23761 cttttccctt ccctctgcca tttttatatt ccttatgtcg tgattataaa aaggaaacgg
    23821 ctatgcaata tcaccgtata ccccacagtt cgctggaagt cagcacgctg gggcttggca
    23881 cgatgacgtt tggtgaacag aacagcgaag ccgacgccca cgcacaactc gactatgccg
    23941 tcgctcaggg cattaacctt atcgacgttg ccgaaatgta cccagtacct ccgcgccccg
    24001 aaacgcaagg gttaaccgaa acctacgtcg gcaactggct ggcgaaacat ggcagccgcg
    24061 aaaagttaat tatcgcctcc aaagtgagcg gaccgtcgcg caataatgac aagggcatcc
    24121 gcccggatca ggcgctggat cggaagaata tccgcgaagc gctgcatgac agcctcaagc
    24181 gcctacagac tgattacctc gatctttatc aggtgcactg gccgcagcgc ccgaccaact
    24241 gcttcggcaa actcggttat agctggacgg attctgcgcc tgcggtttcg ctgctggata
    24301 cgctggacgc actggcagag taccaacgcg cgggaaaaat tcgttatatc ggcgtgtcga
    24361 acgaaactgc atttggcgta atgcgctacc tgcatctggc ggacaaacac gatctgccgc
    24421 gtattgtcac cattcagaac ccttacagtc tgttaaaccg cagttttgaa gtaggtctgg
    24481 cagaagtcag ccagtatgaa ggggtcgaac tgctggccta ttcgtgcctg ggtttcggca
    24541 cgctgaccgg gaaatatctc aatggtgcaa aacccgctgg cgcacgtaat acgctcttta
    24601 gtcggttcac ccgctatagc ggtgagcaaa cgcaaaaagc cgtcgcggcg tatgttgata
    24661 tcgccagacg tcatggcctg gcccctgctc agatggcgct cgcgtttgta cgccgtcaac
    24721 cgtttgttgc cagcactctg ctgggcgcaa ccacgatgga tcagctgaaa actaacatcg
    24781 aaagtttgca tctggagtta agcgaagacg tattagctga aattgaagcg gtgcatcagg
    24841 tttatactta tccggcacca taagattaac gccggttaaa accggcgtta aatattaatg
    24901 acggcgctgc cagatccaca gcgccgttat tgccagcgca aacagcgcac cgaagccaat
    24961 gccaatgggc acgaccggga tgcctatcat taccgccagc gagtaaatgc ccagcatcaa
    25021 caacatggcg ctgttttcgc caaggttttg tactgcaatc gcattccccg ccccgacgct
    25081 ttttttaccc cgctcctgta gcaacgcatt gagcggaacg acaaaaaagc cccccatcac
    25141 gccaatcagc atcaacaagg cataggctgg cagcagctcg tgttgcaggg aaaaaatcag
    25201 taccaccacg ccaatcaaaa tcccggctgg catacagcgt gacacggttt ccagcgtaac
    25261 taacttcgct gccgcacctg cgccaaccac gataccaatc gctaccatcg cgttgagata
    25321 ggtgggcgta gcgttatcgg taatgcccag cgccaccggt acccacagca ccaacaggaa
    25381 acgcagcgtg acacccgctc cccagaataa actggtgccc accagcgaaa aacgcgtttc
    25441 accattgcgc catagcgagg tgcaggcatt caggaaactg cgggtcatgt tgatgagatt
    25501 ccaggactgc cccggacgcg ccgccgccag tttgggaatg tagatattgg caacgaccgc
    25561 accaccgtag gccagtgcgc atgcggccag ggcgacgagg acatgccagt cagccagcac
    25621 accaccggct acggaaccga gcaaaatcgc cgctatggta gaagcttcca ttaaaccgtt
    25681 agctttcact aacttactac ccgtggttaa ttcgccgaga ataccgtatt tcgccggtga
    25741 ataggctgca gcaccaacac ccaccagcgt atagccgaga aacggattga taccaaagca
    25801 gatactggct gcgcccagca gcttcaggcc gttggcaaac atcatcaccc ggcctttggc
    25861 gaagctatcc gccacctgcc cgacaaacgg cgcaaaaaga atgtaagcac ctacaaacac
    25921 catttgcagg atgggctggc tccactccgg atagaactgc gctttcagta acgccagagt
    25981 ggcaaacagt agggcattat cgccaaacgc agagagaaac tgcgccacga taaccgcttt
    26041 catccccttc gaccacaacg aagtgttagt gtgcactgac tcactcatcg tgttgttccg
    26101 cttcgtctac ccagcttttc aacgtgacaa agtcaggttt gccgctgcca agtaatggca
    26161 tctgtttcag atagcgaata tcgcgcggta cagcaagctc cggcacgccg tgctcgcggg
    26221 catactgttg caacttatcg cgcgtcagtt cgttatctgt ggtgaaaagc accagtgcct
    26281 cgcctttgct ggcatcgctc ttaatcgcag tggcatggac tttatctggc gaaacaccaa
    26341 gtgccagttg ttccaccatt tccagcgaca ccatttcgcc tgcaattttg gcaaagcgtt
    26401 ttgcgcggcc ctgaatctgc acaaagccct gctcgtcaaa acgcacaata tcgccagtgt
    26461 cataccagcc gcgctccatt tcgccgcgaa cattctcggc ggtgggcact tccagtacac
    26521 ctggcttctc cacccgcaga tagccgttca ttatgttcgg ccctttcagt tgcaggcgtc
    26581 cgccctcttc gataccagga accgacaaca ggcgcgcatc cattcctggt agaatacgcc
    26641 ctaccgtacc gggtttcgcc gccatcggta cgttgataga aacgacaggc gcgcactcgg
    26701 ttacaccata gccttcaagg atgcgcaggc caaatttatc ctgccaaagc tgtttggtac
    26761 tttcttgtag tttttctgcg cctgccacca catagcgtag acgatagaag tcatacgggt
    26821 tggcgaaacg cgcgtagtga ccgaggaaag tcgaggtacc gaacaacacg gtgcaactgc
    26881 ggtcatacac caactccggc acaatgcggt aatgcagcgg gcttggataa aggaacactt
    26941 ctgcacctgt aagcagtggc gtaaacaggc ctaccgtcag cccaaaggag tgaaacagcg
    27001 gtaatgccga cataaagcga tcgttggtgg tgaagtcggc aatcgtttta atctgctcga
    27061 cgttcgccag aatgctttta tggctatgga cgacgccttt tggatggcct tcagaaccgg
    27121 aggtaaagag gatcagcgcc tcttcttccg gctgctgttt tacctgcgcc aggcgcggca
    27181 tcagcaaatg agcgaagatc catactttgt cggcagtggt gacatctgct tttaaatctt
    27241 ccagatagac ccagcgcacc tgagtaagtt gctccggcag atgccagagt ttgcctttat
    27301 cgagaaactg gcgggaagtg aagatggttt tgatttcagc cgccgtaata gcactggtca
    27361 gcccttttac cccagcagtg tagttcatca ttgcgggaat gcggcgacgc gcgatggccc
    27421 caaaaatcac tgccgcactg atgcctgcat tgggcagcat taagccgatg cgttcgcctt
    27481 caacactgta tttttcaagg atgcgtccaa caaacagcgt tttcgtaagc aatttgcgat
    27541 aggagtctgg ggtaaagttg acgtcttcga cacatttctt cccggctccg aagcggtaca
    27601 ttgcactcag taaagattcg tacagagttt cacgcgggcg caccgccata cgcgcttcca
    27661 tcattatttg atgcagcatt tcgccagcga ttttgcgacg gtcacgggca cgcggcgcat
    27721 ccggcatcgc cacctgcgtt ggtggcaaaa tatgcagagt aatttgcggg aacaagcgac
    27781 gtttaaccag acctttcagg cggctgaagt gcgtaagttc cgccccttca atacgcacag
    27841 gaataaccgt tgcaccagac ttcgccgcga caaaacccgc gccatcgtag attttcatca
    27901 gcgagcctgt agtggtgatg cgtccttcag ggaaaatcac cactggtcgg ccctgttcca
    27961 ccagacgtac cagatgttta atagccatag gttgcgtcgg gtcgagagga acaaagtcga
    28021 taaatgattt cagccaacgc atataccact gttggcttat tgaggtgtaa acggcaaaca
    28081 ctggacgcac aggtaaaaac agtccaagca aaatgccatc aataaaagag acgtgattag
    28141 gcgtaattag aacgcgctcg cccttcagtg cctgggtgtc acccgtaacg cgaacgcgat
    28201 acaaaacacg gcacaaattt cgaaaaaagc taaaaagcat tccaactccc tttgctctga
    28261 ttcagtaaaa gcgaatggag ggagattaca cgagataaag aacgcgagcg acagtaaatt
    28321 aggtgcgaaa aaaaacctgc gcatccgcgc aggttggtgc aagagacagg gtacgaagag
    28381 cgtaccgaat aatctcacca atcaatacct ctgggatctt gattgtggtc tgcacgacga
    28441 ctcttcgcca gcgagaaaac gcaaaggaat gaagggaaat gcaacgaggt gtgtaaattg
    28501 tcggttactg ttacagattg atgaccggca aaaaaaaacc tgcgcatctg cgcaggctgg
    28561 tgtaattcat gtgctcaacc cgaagttgac ttcacctatc aatacctctg ggatcaccac
    28621 tttagcaacc tgaagccaaa cgccaccagc ggtcaatcgc aacagcgttt cgcaaagtgt
    28681 aaccaaaggt ttgcaatctc tttttttgtc ttacttattt gcgtttggct cgcattccca
    28741 cgatgaaaac acgccacccc ttgaaccaac gggcgttttc cgtaacactg aaagaatgta
    28801 agcgtttacc tactaaggta ttttcatggc gaccataaag gatgtagccc gactggcagg
    28861 cgtttcagtc gccaccgttt cccgcgtcat taataattca cctaaagcca gtgaagcttc
    28921 ccggctggct gtgcatagtg caatggagtc tcttagctat cacccgaacg ccaacgcccg
    28981 tgcgctggcg cagcagacca ctgaaacggt cggtctgatc gttggtgatg tttccgatcc
    29041 gtttttcggt gcaatggtga aagcggtcga acaggtggct tatcacaccg gtaatttctt
    29101 attgattggc aacggttacc acaacgaaca aaaagagcgt caggctattg agcaactgat
    29161 ccgccatcgc tgtgctgcgt tggtcgtcca tgccaaaatg atcccggatg ccgatttagc
    29221 ctcattaatg aaacaaatgc ccggtatggt gctgatcaac cgtatcctgc ctggctttga
    29281 aaaccgttgt attgctcttg acgatcgtta cggtgcctgg ctggcaacgc gtcatttaat
    29341 tcagcaaggt catacccgca ttggttatct gtgctctaac cactctattt ctgacgccga
    29401 agatcgtctg caagggtatt acgatgccct tgctgaaagt ggtattgcgg ccaatgaccg
    29461 gctggtgaca tttggcgaac cagacgaaag cggcggcgaa caggcaatga ccgagctttt
    29521 gggacgagga agaaatttca ctgcggtagc ctgttataac gattcaatgg cggcgggtgc
    29581 gatgggcgtt ctcaatgata atggtattga tgtaccgggt gagatttcgt taattggctt
    29641 tgatgatgtg ctggtgtcac gctatgtgcg tccgcgcctg accaccgtgc gttacccaat
    29701 cgtgacgatg gcgacccagg ctgccgaact ggctttggcg ctggcggata atcgccctct
    29761 cccggaaatc actaatgtct ttagtccgac gctggtacgt cgtcattcag tgtcaactcc
    29821 gtcgctggag gcaagtcatc atgcaaccag cgactaaccg cagttaaagc aattccagcg
    29881 ccagtaattc ttcgatggtc tggcgacggc gaatcaaccg cgcctgacca ttatcaaaca
    29941 gaacttctgg taacagcgga cggctattgt agttggatga cattgatgcg ccatatgccc
    30001 ctgtatcatg cagtaccaga taatcacccg ccttcacttc cggcaaggcg cgggtttcaa
    30061 catttccccc ttcctgctgg gtaaagacat cgcccgattc acataatggc ccggcgacga
    30121 cggtttccac cgttggcgcg tgttccagag aacgaccatc agctgccagg gcactgatat
    30181 ggtggtaact accgtacatt gccgggcgca tcagatcgtt gaacccggca tcaaccagca
    30241 caaagtggcg gctacccatt tgtttgacgc tccgcacctg agtaattaac acgccagact
    30301 gcgctaccag aaagcgaccc ggttcaattt ccagtttcac agggtggccc aaatggcgag
    30361 cgatttgctc acgcgcggca ttccacagac cataataatg ttcggtatca accgcctctt
    30421 caccctgttg ataaggaata gaaagcccac cgcccgcaga aatagcctgt aaatcctgac
    30481 cgaattcgat gacctgacgt accatagcac cacacacctg ttccagatgg gcataatcaa
    30541 cgccagaacc aatgtgcatg tgaatgccga ccagctgcaa atgatgacgt tgtatcacgt
    30601 ccagtgcggc gggcagatcg gtgtaccaga taccgtgctt gctgttttcg ccaccggtat
    30661 tggttttttg gctatgtccg tgaccaaacc ccgggttaac gcgcagccat acccgatgcc
    30721 ctggcgaaac ctggcccagt tggtcgagca tatcaacaga acccgcattc accggaattt
    30781 gcaattcact gacgcgttca agcgtcgcct gatcgataac atctgccgta aaaacaatat
    30841 catcggggtg cgtttgcgga ttgtaacccg ccgccaacgc acgctctatt tcgcctaacg
    30901 agacggaatc cactttcacg ccctgctcac gcattaatcg caaaatatga atattggaac
    30961 aggctttctg tgcaaagcgc accacatcaa actgtttcag cgctgcaatc tgccgacgaa
    31021 taatttgcgc atcgtagacc cacaccgggc agccaaattc agcgggcaaa cgcagcagat
    31081 tttcggcggt gagatcggta tcggtgctga acagtgaatg tggcataaca aactccagat
    31141 gagtgctttt ttatgattac gccacatcat aaaaagaata aaaaatatcg atttatgtcg
    31201 agtctatgca aaaatgatat ggattaccgg attgcgagag agcgctaatg gccgccgtta
    31261 acttacgtca tattgaaatt tttcatgcgg taatgaccgc cggaagcctg actgaggcag
    31321 cacacctgct acacacctca cagccaaccg tcagccgcga actggcgcgc tttgagaagg
    31381 tgatcgggct aaaattgttt gagcgcgtac gtggacgatt acatcctacc gtgcaaggac
    31441 tgcgtctgtt tgaagaagtg caacgatcct ggtacggact ggatcgcatt gtcagcgccg
    31501 cagaaagtct gcgcgagttt cgccagggag aactgtctat tgcctgcctg ccggtctttt
    31561 cgcaatcttt tttaccgcag ctcctgcaac cctttctggc acgttatccc gatgtcagct
    31621 taaatatcgt gccccaggaa tcaccgctac ttgaagagtg gctctcggcc cagcgtcatg
    31681 atttaggact cactgagaca ctccatacgc ctgcgggaac agaacgtacc gaattactct
    31741 ctttagatga agtgtgtgtg ttacctccgg gtcatccgct ggcggtaaaa aaggtattaa
    31801 cgccggatga ttttcagggt gagaactaca tcagcctttc ccgtactgac agctatcgcc
    31861 agttgctgga tcagctattt actgaacatc aggttaaacg acgcatgatc gtagaaaccc
    31921 acagcgccgc gtcagtctgc gcaatggtac gggcgggggt aggtgtttcg gtggttaacc
    31981 cgctcaccgc actggattat gcggcaagcg gtttagtggt acggcggttc agtattgcgg
    32041 ttccgttcac cgtcagcctg atccgccccc tgcaccgccc gtcatcagcg ctggttcagg
    32101 cgtttagtga gcatttacaa gcggggttac cgaaactggt cacttctctt gacgcgattt
    32161 tgtcgtcagc tacgacagca taaaagcgac agcatcctcg gcatggatcg ccgcagtatc
    32221 aaacacaggc aaaacactgc gctcttctgg aaccaataaa ccaatttctg tgcagccaaa
    32281 aatgacaccc tgcgccccct gttctgcaag gcgagcaatc acttgcgcat aataagcgcg
    32341 tgacgcttcg gtaaattgcc ccagacacag ttcttcaaaa ataatctgat taattttcgc
    32401 ccgttcatcc gcttcaggaa taagacaatt gatggaaaat tgttccgtca gccgcccgcg
    32461 ataaaaatcc tgttccatgg tgtaacgcgt acccagcagc gccacacgag tcattccggc
    32521 cccggtaatt gcacgtccgg tggcatccgc aatgtgtaag aaaggcagag tgcaacgtga
    32581 ctcaatggca tccgccactt tatgcatcgt attggtacat aacacaatac cttctgcgcc
    32641 cgcccgctgt aagccaagcg ccgcctcagc cagaatgtcc ccggttttat cccattcccc
    32701 gcgacgctgg cactcttcta tttcatgaaa atcgacgcta tgtagcagca cttgcgcaga
    32761 gtgaagccca ccaagccgct gtttaatgcc ttcatttatc agacgatagt aaggaatggt
    32821 ggattcccag ctcattcctc ccagcaaacc aattgttttc attcctctct cctgatgtgt
    32881 gttaccgcaa caaggctgaa taaggaacga gacaaacgcc tcaacggcca ggtgcccgat
    32941 ctctattaac gaaaaaaggg ccggatgtac agcacatccg gcccgtgaaa tcagacgccg
    33001 atatttctca acttctcgcc tgccatcagt ttgcgttcga tatgttccag cgtgacattt
    33061 ttggtttccg gaatgagcca gaaagtgatg cccacaaacg caatgttcag cgcagtgtag
    33121 agccagaacg taccggcagc gccaatgcta tcaagcagtg tcaggaaggt cgcgccgata
    33181 atcatattcg acacccagtt tgtcgtcgtc gaacaggtaa taccgaaatc gcggcatttc
    33241 agcggctgaa tttcagagca caggatccac accactggcg cggcgctcat cgcataaccg
    33301 gcaatacaca tcatcgtcat gccaacagag agccaggaca agccactgga agccgtaccg
    33361 ttatcaaact gcatcaggca atagcccagc accagagtgc ctaacgccat cacgctgaaa
    33421 ccaattttca gagccggttt acgacctgct ttatctaccg taaagaccgc aatgaaggtc
    33481 gcgaacataa aggtcagtcc cacgaccaga gtcgcaatca tctgttgttc tgtggtcgta
    33541 aagcccgcca ttttgaagat acgcggcgca tagtacatga tgatgttcat accggtaaac
    33601 tgctgcatcg cctgcaacaa cataccgaga aacacagcac gacggacgtt acggttgatc
    33661 ttaaacagtg cccaaccgcc ttgttttaac ttcaggcttt cacgaatttc gttgagttct
    33721 tctcgcgctt tttccgacgt atcgcgcagc atacgcaaca cttcttccgc ctcaatatga
    33781 cgcccctttt ccgccagcca gcgcgggcta tttggcagga agaccaccag aataatcagc
    33841 aggactgctg gtaaagcaag aacccccaac attgcgcgcc agttaccgct ataactgaac
    33901 gctgtatcgg ataaaaacgc cagcacgatg ccgagtgtga ccatcaactg gtacatactg
    33961 atcatcttac cgcgaacgtt ttcacttgcc atttcagaaa gatacagagg agcggtgtaa
    34021 gacgcgatcc cgacagcaat gcccagcacc acacgagcgg cgattaacat ctctacgctg
    34081 gtcgcaaaag cggaccctat agaaccgagt acaaacagga tggcccccgc catcaggctg
    34141 tatttacgac ccaggcggaa cgacagccaa ccattaaaca gcgcaccaat tgctgcgccg
    34201 agcatcatgc tactaaccac ccattcctgc aaacgactgg tcagcacaaa gtgatcggta
    34261 atgaacggca acgctccggc gattacgccg atatcaagac caaataacaa tcctgcgacc
    34321 gcagcagcta ccgaaacaaa catattcata cgccgcgtat cccgcaaaga acgtggcgtt
    34381 aaagcagatt ccgtattgat agtaaccatt ttttcctgcc agcagagagt aagacatagt
    34441 gaaaaaatac gtgaacaact cacgcaggtg tcaggtcgga aacagcataa atatggatta
    34501 aattgctgcg acatgtc
//
LOCUS       DAZKHF020000020        32355 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2201S31241
            SAMN43267800-rid25106413.denovo.020, whole genome shotgun sequence.
ACCESSION   DAZKHF020000020 DAZKHF020000000
VERSION     DAZKHF020000020.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267800
            Sequence Read Archive: SRR30307263
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 32355)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 32355)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 32355)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350581.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350581.2
            Genome Coverage       :: 151x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:21:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,652
            CDSs (total)                      :: 5,557
            Genes (coding)                    :: 5,320
            CDSs (with protein)               :: 5,320
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 237
            CDSs (without protein)            :: 237
            Pseudo Genes (ambiguous residues) :: 0 of 237
            Pseudo Genes (frameshifted)       :: 102 of 237
            Pseudo Genes (incomplete)         :: 130 of 237
            Pseudo Genes (internal stop)      :: 52 of 237
            Pseudo Genes (multiple problems)  :: 41 of 237
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..32355
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267800-rid25106413.denovo.020"
                     /serotype="O 26:H11"
                     /isolate="2201S31241"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-01"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            complement(18..93)
                     /locus_tag="ACHOSC_RS04080"
                     /old_locus_tag="pgaptmp_000816"
     tRNA            complement(18..93)
                     /locus_tag="ACHOSC_RS04080"
                     /old_locus_tag="pgaptmp_000816"
                     /product="tRNA-His"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(58..60),aa:His,seq:gtg)
     gene            complement(152..228)
                     /locus_tag="ACHOSC_RS04085"
                     /old_locus_tag="pgaptmp_000817"
     tRNA            complement(152..228)
                     /locus_tag="ACHOSC_RS04085"
                     /old_locus_tag="pgaptmp_000817"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(192..194),aa:Arg,seq:ccg)
     gene            complement(331..1716)
                     /gene="yifK"
                     /locus_tag="ACHOSC_RS04090"
                     /old_locus_tag="pgaptmp_000818"
     CDS             complement(331..1716)
                     /gene="yifK"
                     /locus_tag="ACHOSC_RS04090"
                     /old_locus_tag="pgaptmp_000818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HGU4546806.1"
                     /translation="MADNKPELQRGLEARHIELIALGGTIGVGLFMGAASTLKWAGPS
                     VLLAYIIAGLFVFFIMRSMGEMLFLEPVTGSFAVYAHRYMSPFFGYLTAWSYWFMWMA
                     VGISEITAIGVYVQFWFPEMAQWIPALIAVALVALANLAAVRLYGEIEFWFAMIKVTT
                     IIVMIVIGLGVIFFGFGNGGQSIGFSNLTEHGGFFAGGWKGFLTALCIVVASYQGVEL
                     IGITAGEAKNPQVTLRSAVGKVLWRILIFYVGAIFVIVTIFPWNEIGSNGSPFVLTFA
                     KIGITAAAGIINFVVLTAALSGCNSGMYSCGRMLYALAKNRQLPAAMAKVSRHGVPVA
                     GVAVSIAILLIGSCLNYIIPNPQRVFVYVYSASVLPGMVPWFVILISQLRFRRAHKAA
                     IASHPFRSILFPWANYVTMAFLICVLIGMYFNEDTRMSLFVGIIFMLAVTAIYKVFGL
                     NRHGKAHKLEE"
     gene            complement(1907..2647)
                     /gene="wecG"
                     /locus_tag="ACHOSC_RS04095"
                     /old_locus_tag="pgaptmp_000819"
     CDS             complement(1907..2647)
                     /gene="wecG"
                     /locus_tag="ACHOSC_RS04095"
                     /old_locus_tag="pgaptmp_000819"
                     /EC_number="2.4.1.180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047241 - lipopolysaccharide
                     N-acetylmannosaminouronosyltransferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide
                     N-acetylmannosaminouronosyltransferase"
                     /protein_id="HGU4546807.1"
                     /translation="MNNNTMAPTYTLRGLQLIGWRDMQHALDYLFADGQLKQGTLVAI
                     NAEKMLTIEDNAEVRELINAAEFKYADGISVVRSVRKKYPQAQVSRVAGADLWEELMA
                     RAGKEGTPVFLVGGKPEVLAQTEAKLRNQWNVNIVGSQDGYFKPEQRQALFERIHASG
                     AQIVTVAMGSPKQEIFMRDCRLVHPDALYMGVGGTYDVFTGHVKRAPKIWQTLGLEWL
                     YRLLSQPSRIKRQLRLLRYLRWHYTGNL"
     gene            complement(2650..4002)
                     /gene="wzyE"
                     /locus_tag="ACHOSC_RS04100"
                     /old_locus_tag="pgaptmp_000820"
     CDS             complement(2650..4002)
                     /gene="wzyE"
                     /locus_tag="ACHOSC_RS04100"
                     /old_locus_tag="pgaptmp_000820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009246 - enterobacterial common antigen
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ECA oligosaccharide polymerase"
                     /protein_id="HGU4546808.1"
                     /translation="MSLLQFSGLFVVWLLCTLFIATLTWFEFRRVRFNFNVFFSLLFL
                     LTFFFGFPLTSVLVFRFDVGVAPPEILLQALLSAGCFYAVYYVTYKTRLRKRVADVPR
                     RPLFTMNRVETNLTWVILMGIALVSVGIFFMHNGFLLFRLNSYSQIFSSEVSGVALKR
                     FFYFFIPAMLVVYFLRQDSKAWLFFLVSTVAFGLLTYMIVGGTRANIIIAFAIFLFIG
                     IIRGWISLWMLAAAGVLGIVGMFWLALKRYGMNVSGDEAFYTFLYLTRDTFSPWENLA
                     LLLQNYDNIDFQGLAPIVRDFYVFIPSWLWPGRPSMVLNSANYFTWEVLNNHSGLAIS
                     PTLIGSLVVMGGALFIPLGAIVVGLIIKWFDWLYELGNREPNRYKAAILHSFCFGAIF
                     NMIVLAREGLDSFVSRVVFFIVVFGACLMIAKLLYWLFESAGLIHKRTKSSLRTQVEG
                     "
     gene            complement(3999..5078)
                     /gene="rffT"
                     /locus_tag="ACHOSC_RS04105"
                     /old_locus_tag="pgaptmp_000821"
                     /gene_synonym="wecF"
     CDS             complement(3999..5078)
                     /gene="rffT"
                     /locus_tag="ACHOSC_RS04105"
                     /old_locus_tag="pgaptmp_000821"
                     /gene_synonym="wecF"
                     /EC_number="2.4.1.325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TDP-N-acetylfucosamine:lipid II
                     N-acetylfucosaminyltransferase"
                     /protein_id="HGU4546809.1"
                     /translation="MTVLIHVLGSDIPHHNRTVLRFFNDALAATSEHAREFMVVGKDD
                     GLSDSCPALSVQFFPGKKSLAEAVIAKAKANRQQRFFFHGQFNPTLWLALLSGGIKPS
                     QFFWHIWGADLYELSSGLRYKLFYPLRRLAQKRVGCVFATRGDLSFFAKTHPKVRGEL
                     LYFPTRMDPSLNTMANDRQREGKMTILVGNSGDLSNEHVAALRAVHQQFGDTVKVVVP
                     MGYPPNNEAYIEEVRQAGLELFSEENLQILSEKLEFDAYLALLRQCDLGYFIFARQQG
                     IGTLCLLIQAGIPCVLNRENPFWQDMTEQHLPVLFTTDDLNEDIVREAQRQLASVDKN
                     TIAFFSPNYLQGWQRALAIAAGEGA"
     gene            complement(5075..6325)
                     /gene="wzxE"
                     /locus_tag="ACHOSC_RS04110"
                     /old_locus_tag="pgaptmp_000822"
     CDS             complement(5075..6325)
                     /gene="wzxE"
                     /locus_tag="ACHOSC_RS04110"
                     /old_locus_tag="pgaptmp_000822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009246 - enterobacterial common antigen
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipid III flippase WzxE"
                     /protein_id="HGU4546810.1"
                     /translation="MSLAKASLWTAASTLVKIGAGLLVGKLLAVSFGPAGLGLAANFR
                     QLITVLGVLAGAGIFNGVTKYVAQYHDNPQQLRRVVGTSSAMVLGFSTLMALVFVLAA
                     APISQGLFGNTDYQGLVRLVALVQMGIAWGNLLLALMKGFRDAAGNALSLIVGSLIGV
                     LAYYVSYRLGGYEGALLGLALIPALVVIPATIMLIKRGVIPLSYLKPSWDNGLAGQLS
                     KFTLMALITSVTLPVAYIMMRKLLAAQYSWDEVGIWQGVSSISDAYLQFITASFSVYL
                     LPTLSRLTEKRDITREVVKSLKFVLPAVAAASFTVWLLRDFAIWLLLSNKFTAMRDLF
                     AWQLVGDVLKVGAYVFGYLVIAKASLRFYILAEISQFTLLMVFAHWLIPAHGALGAAQ
                     AYMATYIVYFSLCCGVFLLWRRRA"
     gene            complement(6327..7457)
                     /gene="rffA"
                     /locus_tag="ACHOSC_RS04115"
                     /old_locus_tag="pgaptmp_000823"
                     /gene_synonym="fcnA"
                     /gene_synonym="wecE"
     CDS             complement(6327..7457)
                     /gene="rffA"
                     /locus_tag="ACHOSC_RS04115"
                     /old_locus_tag="pgaptmp_000823"
                     /gene_synonym="fcnA"
                     /gene_synonym="wecE"
                     /EC_number="2.6.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019180 -
                     dTDP-4-amino-4,6-dideoxygalactose transaminase activity
                     [Evidence IEA];
                     GO_process: GO:0009246 - enterobacterial common antigen
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-amino-4,6-dideoxygalactose transaminase"
                     /protein_id="HGU4546811.1"
                     /translation="MIPFNAPPVVGTELDYMQSAMGSGKLCGDGGFTRRCQQWLEQRF
                     GSAKVLLTPSCTASLEMAALLLDIQPGDEVIMPSYTFVSTANAFVLRGAKIVFVDVRP
                     DTMNIDETLIEAAITDKTRVIVPVHYAGVACEMDTIMALAKKHNLFVVEDAAQGVMST
                     YKGRALGTIGHIGCFSFHETKNYTAGGEGGATLINDKALIERAEIIREKGTNRSQFFR
                     GQVDKYTWRDIGSSYLMSDLQAAYLWAQLEAADRINQQRLALWQNYYDALAPLAKAGR
                     IELPSIPDGCVQNAHMFYIKLRDIDDRSALINFLKEAEIMAVFHYIPLHGCPAGERFG
                     EFHGEDRYTTKESERLLRLPLFYNLSPVNQRTVIATLLNYFS"
     gene            complement(7462..8136)
                     /gene="rffC"
                     /locus_tag="ACHOSC_RS04120"
                     /old_locus_tag="pgaptmp_000824"
                     /gene_synonym="wecD"
     CDS             complement(7462..8136)
                     /gene="rffC"
                     /locus_tag="ACHOSC_RS04120"
                     /old_locus_tag="pgaptmp_000824"
                     /gene_synonym="wecD"
                     /EC_number="2.3.1.210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026256.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-amino-4,6-dideoxy-D-galactose
                     acyltransferase"
                     /protein_id="HGU4546812.1"
                     /translation="MPVRASIEPLTWENAFFGVNSAIVRITSEAPLLTPDVLAPWSRV
                     QAKIAASNTGELDALQQLGFSLVEGEVDLALPVNNVSDSGAVVAQETDIPALRQLASA
                     AFAQSRFRAPWYAPDASRRFYAQWIENAVRGTFDHQCLILRAASGGIRGYVSLRELNA
                     TDARIGLLAGRGVGAELMQTALNWAYARGKTTLRVATQMGNTAALKRYIQSGANVEST
                     AYWLYR"
     gene            complement(8114..8995)
                     /gene="rfbA"
                     /locus_tag="ACHOSC_RS04125"
                     /old_locus_tag="pgaptmp_000825"
     CDS             complement(8114..8995)
                     /gene="rfbA"
                     /locus_tag="ACHOSC_RS04125"
                     /old_locus_tag="pgaptmp_000825"
                     /EC_number="2.7.7.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005133346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008879 - glucose-1-phosphate
                     thymidylyltransferase activity [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0045226 - extracellular polysaccharide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate thymidylyltransferase RfbA"
                     /protein_id="HGU4546813.1"
                     /translation="MKGIILAGGSGTRLHPITRGVSKQLLPIYDKPMIYYPLSVLMLA
                     GIREILIITTPEDKGYFQRLLGDGSEFGIQLEYAEQPSPDGLAQAFIIGETFLNGEPS
                     CLVLGDNIFFGQGFSPKLRHVAARTEGATVFGYQVMDPERFGVVEFDDNFRAISLEEK
                     PKQPKSNWAVTGLYFYDSKVVEYAKQVKPSERGELEITSINQMYLEAGNLTVELLGRG
                     FAWLDTGTHDSLIEASTFVQTVEKRQGFKIACLEEIAWRNGWLDDEGVKRAASSLAKT
                     GYGQYLLELLRARPRQY"
     gene            complement(9014..10081)
                     /gene="rffG"
                     /locus_tag="ACHOSC_RS04130"
                     /old_locus_tag="pgaptmp_000826"
     CDS             complement(9014..10081)
                     /gene="rffG"
                     /locus_tag="ACHOSC_RS04130"
                     /old_locus_tag="pgaptmp_000826"
                     /EC_number="4.2.1.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008460 - dTDP-glucose 4,6-dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009225 - nucleotide-sugar metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-glucose 4,6-dehydratase"
                     /protein_id="HGU4546814.1"
                     /translation="MRKILITGGAGFIGSALVRYIINETSDAVVVVDKLTYAGNLMSL
                     APVAQSEHFAFEKVDICDRAELARVFTEHQPDCVMHLAAESHVDRSIDGPAAFIETNI
                     VGTYTLLEAARAYWNTLTEDKKSAFRFHHISTDEVYGDLHSTDDFFTETTPYAPSSPY
                     SASKASSDHLVRAWLRTYGLPTLITNCSNNYGPYHFPEKLIPLMILNALAGKPLPVYG
                     NGQQIRDWLYVEDHARALYCVATTGKVGETYNIGGHNERKNLDVVETICELLEELAPN
                     KPHGVAHYRDLITFVADRPGHDLRYAIDASKIARELGWLPQETFESGMRKTVQWYLAN
                     ESWWKQVQDGSYQGERLGLKG"
     gene            complement(10081..11343)
                     /gene="wecC"
                     /locus_tag="ACHOSC_RS04135"
                     /old_locus_tag="pgaptmp_000827"
     CDS             complement(10081..11343)
                     /gene="wecC"
                     /locus_tag="ACHOSC_RS04135"
                     /old_locus_tag="pgaptmp_000827"
                     /EC_number="1.1.1.336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_function: GO:0089714 - UDP-N-acetyl-D-mannosamine
                     dehydrogenase activity [Evidence IEA];
                     GO_process: GO:0009246 - enterobacterial common antigen
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetyl-D-mannosamine dehydrogenase"
                     /protein_id="HGU4546815.1"
                     /translation="MSFATISVIGLGYIGLPTAAAFASRQKQVIGVDINQHAVDTINR
                     GEIHIVEPDLASVVKTAVEGGFLRASTTPVEADAWLIAVPTPFKGDHEPDMTYVESAA
                     RSIAPVLKKGALVILESTSPVGSTEKMAEWLAEMRPDLTFPQQVGEQADVNIAYCPER
                     VLPGQVMVELIKNDRVIGGMTPVCSTRASELYKIFLEGECVVTNSRTAEMCKLTENSF
                     RDVNIAFANELSLICADQGINVWELIRLANRHPRVNILQPGPGVGGHCIAVDPWFIVA
                     QNPQQARLIRTAREVNDHKPFWVIDQVKAAVADCLAATDKRASELKIACFGLAFKPNI
                     DDLRESPAMEIAELIAQWHSGETLVVEPNIHQLPKKLTGLCTLAQLDEALATADVLVM
                     LVDHSQFKVINGDNVHQQYVVDAKGVWR"
     gene            complement(11340..12470)
                     /gene="wecB"
                     /locus_tag="ACHOSC_RS04140"
                     /old_locus_tag="pgaptmp_000828"
     CDS             complement(11340..12470)
                     /gene="wecB"
                     /locus_tag="ACHOSC_RS04140"
                     /old_locus_tag="pgaptmp_000828"
                     /EC_number="5.1.3.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312746.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008761 - UDP-N-acetylglucosamine
                     2-epimerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="non-hydrolyzing UDP-N-acetylglucosamine
                     2-epimerase"
                     /protein_id="HGU4546816.1"
                     /translation="MKVLTVFGTRPEAIKMAPLVHALAKDPFFEAKVCVTAQHREMLD
                     QVLKLFSIVPDYDLNIMQPGQGLTEITCRILEGLKPILAEFKPDVVLVHGDTTTTLAT
                     SLAAFYQRIPVGHVEAGLRTGDLYSPWPEEANRTLTGHLAMYHFSPTETSRQNLLREN
                     VADSRIFITGNTVIDALLWVRDQVMSSDTLRSELAANYPFIDPDKKMILVTGHRRESF
                     GRGFEEICHALADIATTHQDIQIVYPVHLNPNVREPVNRILGHVKNVILIDPQEYLPF
                     VWLMNHAWLILTDSGGIQEEAPSLGKPVLVMRDTTERPEAVTAGTVRLVGTDKQRIVE
                     EVTRLLKDENEYQTMSRAHNPYGDGQACSRILEALKNNRISL"
     gene            complement(12526..13572)
                     /gene="wzzE"
                     /locus_tag="ACHOSC_RS04145"
                     /old_locus_tag="pgaptmp_000829"
     CDS             complement(12526..13572)
                     /gene="wzzE"
                     /locus_tag="ACHOSC_RS04145"
                     /old_locus_tag="pgaptmp_000829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709589.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009246 - enterobacterial common antigen
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ECA polysaccharide chain length modulation
                     protein"
                     /protein_id="HGU4546817.1"
                     /translation="MTQPMPGKPAEDAENELDIRGLFRTLWAGKLWIIGMGLAFALIA
                     LAYTFFARQEWSATAITDRPTVNMLGGYYSQQQFLRNLDVRSNMASADQPSVMDEAYK
                     EFVMQLASWDTRREFWLQTDYYKQRMVGNSKADAALLDEMINNIQFIPGDFTRAVNDS
                     VKLIAETAPDANNLLRQYVAFASQRAASHLNEELKGAWAARTIQMKAQVKRQEEVAKA
                     IYDRRMNSIEQALKIAEQHNISRSATDVPAEELPDSEMFLLGRPMLQARLENLQAVGP
                     AFDLDYDQNRAMLNTLNVGPTLDPRFQTYRYLRTPEEPVKRDSPRRAFLMIMWGIVGG
                     LIGAGVALTRRCSK"
     gene            complement(13584..14687)
                     /gene="wecA"
                     /locus_tag="ACHOSC_RS04150"
                     /old_locus_tag="pgaptmp_000830"
     CDS             complement(13584..14687)
                     /gene="wecA"
                     /locus_tag="ACHOSC_RS04150"
                     /old_locus_tag="pgaptmp_000830"
                     /EC_number="2.7.8.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009276 - Gram-negative-bacterium-type
                     cell wall [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0016780 - phosphotransferase activity, for
                     other substituted phosphate groups [Evidence IEA];
                     GO_function: GO:0030145 - manganese ion binding [Evidence
                     IEA];
                     GO_function: GO:0036380 -
                     UDP-N-acetylglucosamine-undecaprenyl-phosphate
                     N-acetylglucosaminephosphotransferase activity [Evidence
                     IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009246 - enterobacterial common antigen
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine--undecaprenyl-phosphate
                     N-acetylglucosaminephosphotransferase"
                     /protein_id="HGU4546818.1"
                     /translation="MNLLTVSTDLISIFLFTTLFLFFARKVAKKVGLVDKPNFRKRHQ
                     GLIPLVGGISVYAGICFTFGIVDYYIPHASLYLACAGVLVFIGALDDRFDISVKIRAT
                     IQAAVGIVMMVFGKLYLSSLGYIFGSWEMVLGPFGYFLTLFAVWAAINAFNMVDGIDG
                     LLGGLSCVSFAAIGMILWFDGQTSLAIWCFAMIAAILPYIMLNLGILGRRYKVFMGDA
                     GSTLIGFTVIWILLETTQGKTHPISPVTALWIIAIPLMDMVAIMYRRLRKGMSPFSPD
                     RQHIHHLIMRAGFTSRQAFVLITLAAALLASIGVLAEYSHFVPEWVMLVLFLLAFFLY
                     GYCIKRAWKVARFIKRVKRRLRRNRGGSPNLTK"
     gene            complement(14927..16186)
                     /gene="rho"
                     /locus_tag="ACHOSC_RS04155"
                     /old_locus_tag="pgaptmp_000831"
     CDS             complement(14927..16186)
                     /gene="rho"
                     /locus_tag="ACHOSC_RS04155"
                     /old_locus_tag="pgaptmp_000831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017492510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008186 - ATP-dependent activity, acting
                     on RNA [Evidence IEA];
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor Rho"
                     /protein_id="HGU4546819.1"
                     /translation="MNLTELKNTPVSELITLGENMGLENLARMRKQDIIFAILKQHAK
                     SGEDIFGDGVLEILQDGFGFLRSADSSYLAGPDDIYVSPSQIRRFNLRTGDTISGKIR
                     PPKEGERYFALLKVNEVNFDKPENARNKILFENLTPLHANSRLRMERGNGSTEDLTAR
                     VLDLASPIGRGQRGLIVAPPKAGKTMLLQNIAQSIAYNHPDCVLMVLLIDERPEEVTE
                     MQRLVKGEVVASTFDEPASRHVQVAEMVIEKAKRLVEHKKDVIILLDSITRLARAYNT
                     VVPASGKVLTGGVDANALHRPKRFFGAARNVEEGGSLTIIATALIDTGSKMDEVIYEE
                     FKGTGNMELHLSRKIAEKRVFPAIDYNRSGTRKEELLTTQEELQKMWILRKIIHPMGE
                     IDAMEFLINKLAMTKTNDDFFEMMKRS"
     gene            complement(16271..16372)
                     /gene="rhoL"
                     /locus_tag="ACHOSC_RS04160"
                     /old_locus_tag="pgaptmp_000832"
     CDS             complement(16271..16372)
                     /gene="rhoL"
                     /locus_tag="ACHOSC_RS04160"
                     /old_locus_tag="pgaptmp_000832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rho operon leader peptide RhoL"
                     /protein_id="HGU4546820.1"
                     /translation="MRSEQISGSSLNPSCRFSSAYSPVTRQRKDMSR"
     gene            complement(16513..16842)
                     /gene="trxA"
                     /locus_tag="ACHOSC_RS04165"
                     /old_locus_tag="pgaptmp_000833"
     CDS             complement(16513..16842)
                     /gene="trxA"
                     /locus_tag="ACHOSC_RS04165"
                     /old_locus_tag="pgaptmp_000833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017375425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015035 - protein-disulfide reductase
                     activity [Evidence IEA];
                     GO_process: GO:0006662 - glycerol ether metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin TrxA"
                     /protein_id="HGU4546821.1"
                     /translation="MSDKIIHLTDDSFDTDVLKADGAILVDFWAEWCGPCKMIAPILD
                     EIADEYQGKLTVAKLNIDQNPGTAPKYGIRGIPTLLLFKNGEVAATKVGALSKGQLKE
                     FLDANLA"
     gene            16973..18238
                     /gene="rhlB"
                     /locus_tag="ACHOSC_RS04170"
                     /old_locus_tag="pgaptmp_000834"
     CDS             16973..18238
                     /gene="rhlB"
                     /locus_tag="ACHOSC_RS04170"
                     /old_locus_tag="pgaptmp_000834"
                     /EC_number="3.6.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003724 - RNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RNA helicase RhlB"
                     /protein_id="HGU4546822.1"
                     /translation="MSKTHLTEQKFSDFALHPKVVEALEKKGFHNCTPIQALALPLTL
                     AGRDVAGQAQTGTGKTMAFLTSTFHYLLSHPAIADRKVNQPRALIMAPTRELAVQIHA
                     DAEPLAEATGLKLGLAYGGDGYDKQLKVLESGVDILIGTTGRLIDYAKQNHINLGAIQ
                     VVVLDEADRMYDLGFIKDIRWLFRRMPPANQRLNMLFSATLSYRVRELAFEQMNNAEY
                     IEVEPEQKTGHRIKEELFYPSNEEKMRLLQTLIEEEWPDRAIIFANTKYRCEEIWGHL
                     AADGHRVGLLTGDVAQKKRLRILDEFTRGDLDILVATDVAARGLHIPAVTHVFNYDLP
                     DDCEDYVHRIGRTGRAGASGHSISLACEEYALNLPAIETYIGHSIPVSKYNPDALMTD
                     LPKPLRLTRPRTGNGPRRTGAPRNRRRSG"
     gene            18372..19856
                     /gene="gppA"
                     /locus_tag="ACHOSC_RS04175"
                     /old_locus_tag="pgaptmp_000835"
     CDS             18372..19856
                     /gene="gppA"
                     /locus_tag="ACHOSC_RS04175"
                     /old_locus_tag="pgaptmp_000835"
                     /EC_number="3.6.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008894 -
                     guanosine-5'-triphosphate,3'-diphosphate diphosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0015974 - guanosine pentaphosphate
                     catabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="guanosine-5'-triphosphate,3'-diphosphate
                     diphosphatase"
                     /protein_id="HGU4546823.1"
                     /translation="MGSTSSLYAAIDLGSNSFHMLVVREVAGSIQTLTRIKRKVRLAA
                     GLNSENALSNEAMERGWQCLRLFAERLQDIPPSQIRVVATATLRLAVNAGDFIAKAQE
                     ILGCPVQVISGEEEARLIYQGVAHTTGGADQRLVVDIGGASTELVTGTGAQTTSLFSL
                     SMGCVTWLERYFADRNLGQENFDAAEKAAREVLRPVADELRYHGWKVCVGASGTVQAL
                     QEIMMAQGMDERITLEKLQQLKQRAIHCGRLEELEIDGLTLERALVFPSGLAILIAIF
                     TELNIQCMTLAGGALREGLVYGMLHLAVEQDIRSRTLRNIQRRFMIDIDQAQRVAKVA
                     ANFFDQVEKEWHLEAISRDLLISACQLHEIGLSVDFKQAPQHAAYLVRNLDLPGFTPA
                     QKKLLATLLLNQTNPVDLSSLHQQNAVPPRVAEQLCRLLRLAIIFASRRRDDLVPEMT
                     LQANHELLTLTLPQGWLTQHPLGKEIIAQESQWQSYVHWPLEVH"
     gene            complement(19903..21924)
                     /gene="rep"
                     /locus_tag="ACHOSC_RS04180"
                     /old_locus_tag="pgaptmp_000836"
     CDS             complement(19903..21924)
                     /gene="rep"
                     /locus_tag="ACHOSC_RS04180"
                     /old_locus_tag="pgaptmp_000836"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_process: GO:0006268 - DNA unwinding involved in DNA
                     replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase Rep"
                     /protein_id="HGU4546824.1"
                     /translation="MRLNPGQQQAVEFVTGPCLVLAGAGSGKTRVITNKIAHLIRGCG
                     YQARHIAAVTFTNKAAREMKERVGQTLGRKEARGLMISTFHTLGLDIIKREYAALGMK
                     ANFSLFDDTDQLALLKELTEGLIEDDKVLLQQLISTISNWKNDLKTPAQAAAEAKGER
                     DRIFAHCYGLYDAHLKACNVLDFDDLILLPTLLLQRNEEVRERWQNKIRYLLVDEYQD
                     TNTSQYELVKLLVGSRARFTVVGDDDQSIYSWRGARPQNLVLLSQDFPALKVIKLEQN
                     YRSSGRILKAANILIANNPHVFEKRLFSELGYGTELKVLSANNEEHEAERVTGELIAH
                     HFVNKTQYKDYAILYRGNHQSRVFEKFLMQNRIPYKISGGTSFFSRPEIKDLLAYLRV
                     LTNPDDDSAFLRIVNTPKREIGPATLKKLGEWAMTRNKSMFTASFDMGLSQTLSGRGY
                     EALTRFTHWLAEIQRLAEREPIAAVRDLIHGMDYESWLYETSPSPKAAEMRMKNVNQL
                     FSWMTEMLEGSELDEPMTLTQVVTRFTLRDMMERGESEEELDQVQLMTLHASKGLEFP
                     YVYMVGMEEGFLPHQSSIDEDNIDEERRLAYVGITRAQKELTFTLCKERRQYGELVRP
                     EPSRFLLELPQDDLIWEQERKVVSAEERMQKGQSHLANLKAMMAAKRGK"
     gene            22141..22590
                     /locus_tag="ACHOSC_RS04185"
                     /old_locus_tag="pgaptmp_000837"
     CDS             22141..22590
                     /locus_tag="ACHOSC_RS04185"
                     /old_locus_tag="pgaptmp_000837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system PemK/MazF family
                     toxin"
                     /protein_id="HGU4546825.1"
                     /translation="MAINFSPKVGEIQECNFGNYPVIQNGQFSTTYYDGRIPPEMIKN
                     RLVVVLNGKINGNACIVVPLSTTRDHDKLNRGMHVEIASNVINGLQFFDQQIRWAKAD
                     LVQQVSRNRLNRARTYRGYLNQCLPHELVADIQRAVIKSINAISLIN"
     gene            22789..23070
                     /gene="ppiC"
                     /locus_tag="ACHOSC_RS04190"
                     /old_locus_tag="pgaptmp_000838"
     CDS             22789..23070
                     /gene="ppiC"
                     /locus_tag="ACHOSC_RS04190"
                     /old_locus_tag="pgaptmp_000838"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004097481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase PpiC"
                     /protein_id="HGU4546826.1"
                     /translation="MAKTAAALHILVKEEKLALDLLEQIKNGADFGKLAKKHSICPSG
                     KRGGDLGEFRQGQMVPAFDKVVFSCPVLEPTGPLHTQFGYHIIKVLYRN"
     gene            complement(23157..24632)
                     /gene="ilvC"
                     /locus_tag="ACHOSC_RS04195"
                     /old_locus_tag="pgaptmp_000839"
     CDS             complement(23157..24632)
                     /gene="ilvC"
                     /locus_tag="ACHOSC_RS04195"
                     /old_locus_tag="pgaptmp_000839"
                     /EC_number="1.1.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005133363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004455 - ketol-acid reductoisomerase
                     activity [Evidence IEA];
                     GO_process: GO:0009082 - branched-chain amino acid
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ketol-acid reductoisomerase"
                     /protein_id="HGU4546827.1"
                     /translation="MANYFNTLNLRQQLAQLGKCRFMGRDEFADGASYLQGKKVVIVG
                     CGAQGLNQGLNMRDSGLDISYALRKEAIAEKRASWRKATENGFKVGTYEELIPQADLV
                     VNLTPDKQHSDVVRTVQPLMKDGAALGYSHGFNIVEVGEQIRKDITVVMVAPKCPGTE
                     VREEYKRGFGVPTLIAVHPENDPKGEGMAIAKAWAAATGGHRAGVLESSFVAEVKSDL
                     MGEQTILCGMLQAGSLLCFDKLVEEGTDPAYAEKLIQFGWETITEALKQGGITLMMDR
                     LSNPAKLRAYALSEQLKEIMAPLFQKHMDDIISGEFSSGMMADWANDDKKLLTWREET
                     GKTAFETAPQYEGKIGEQEYFDKGVLMIAMVKAGVELAFETMVDSGIIEESAYYESLH
                     ELPLIANTIARKRLYEMNVVISDTAEYGNYLFSYACVPLLKPFMAELQPGDLGKAIPE
                     GAVDNAQLRDVNEAIRSHAIEQVGKKLRGYMTDMKRIAVAG"
     gene            24782..25675
                     /gene="ilvY"
                     /locus_tag="ACHOSC_RS04200"
                     /old_locus_tag="pgaptmp_000840"
     CDS             24782..25675
                     /gene="ilvY"
                     /locus_tag="ACHOSC_RS04200"
                     /old_locus_tag="pgaptmp_000840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional activator IlvY"
                     /protein_id="HGU4546828.1"
                     /translation="MDLRDLKTFLHLAESRHFGRSARAMHVSPSTLSRQIQRLEEDLG
                     QPLFVRDNRTVTLTEAGEELRVFAQQTLLQYQQLRHTIDQQGPSLSGELHIFCSVTAA
                     YSHLPPILDRFRAEHPSVEIKLTTGDAADAMEKVVTGEADLAIAGKPETLPGAVAFSM
                     LENLAVVLIAPALPCPVRNQVSAEKPDWSTVPFIMADQGPVRRRIELWFRRNKISNPM
                     IYATVGGHEAMVSMVALGCGVALLPEVVLENSPEPVRNRVMILERSDEKTPFELGVCA
                     QKKRLHEPLIEAFWRILPNHK"
     gene            complement(25727..27271)
                     /gene="ilvA"
                     /locus_tag="ACHOSC_RS04205"
                     /old_locus_tag="pgaptmp_000841"
     CDS             complement(25727..27271)
                     /gene="ilvA"
                     /locus_tag="ACHOSC_RS04205"
                     /old_locus_tag="pgaptmp_000841"
                     /EC_number="4.3.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004794 - threonine deaminase activity
                     [Evidence IEA];
                     GO_process: GO:0009097 - isoleucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine ammonia-lyase, biosynthetic"
                     /protein_id="HGU4546829.1"
                     /translation="MADSQPLSGAPEGAEYLRAVLRAPVYEAAQVTPLQKMEKLSSRL
                     DNVILVKREDRQPVHSFKLRGAYAMMAGLTEEQKAHGVITASAGNHAQGVAFSSARLG
                     VKALIVMPTATADIKVDAVRGFGGEVLLHGANFDEAKAKAIELSQQQGFTWVPPFDHP
                     MVIAGQGTLALELLQQDAHLDRVFVPVGGGGLAAGVAVLIKQLMPQIKVIAVEAEDSA
                     CLKAALDAGHPVDLPRVGLFAEGVAVKRIGDETFRLCQEYLDDIITVDSDAICAAMKD
                     LFEDVRAVAEPSGALALAGMKKYIAQHNIRGERLAHILSGANVNFHGLRYVSERCELG
                     EQREALLAVTIPEEKGSFLKFCQLLGGRSVTEFNYRFADAKNACIFVGVRLSRGLEER
                     KEILQMLNDGGYSVVDLSDDEMAKLHVRYMVGGRPSHPLQERLYSFEFPESPGALLRF
                     LNTLGTHWNISLFHYRSHGTDYGRVLAAFELGDHEPDFETRLNELGYDCHDETNNPAF
                     RFFLAG"
     gene            complement(27274..29124)
                     /gene="ilvD"
                     /locus_tag="ACHOSC_RS04210"
                     /old_locus_tag="pgaptmp_000842"
     CDS             complement(27274..29124)
                     /gene="ilvD"
                     /locus_tag="ACHOSC_RS04210"
                     /old_locus_tag="pgaptmp_000842"
                     /EC_number="4.2.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005133366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004160 - dihydroxy-acid dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009082 - branched-chain amino acid
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxy-acid dehydratase"
                     /protein_id="HGU4546830.1"
                     /translation="MPKYRSATTTHGRNMAGARALWRATGMTDADFGKPIIAVVNSFT
                     QFVPGHVHLRDLGKLVAEQIEAAGGVAKEFNTIAVDDGIAMGHGGMLYSLPSRELIAD
                     SVEYMVNAHCADAMVCISNCDKITPGMLMASLRLNIPVIFVSGGPMEAGKTKLSDQII
                     KLDLVDAMIQGADPKVSDSQSDQVERSACPTCGSCSGMFTANSMNCLTEALGLSQPGN
                     GSLLATHADRKQLFLNAGKRIVELTKRYYEQNDESALPRNIASKAAFENAMTLDIAMG
                     GSTNTVLHLLAAAQEAEIDFTMSDIDKLSRKVPQLCKVAPSTQKYHMEDVHRAGGVIG
                     ILGELDRAGLLNRDVKNVLGLTLPQTLEQYDVMLTQDDAVKNMFRAGPAGIRTTQAFS
                     QDCRWDSLDDDRANGCIRSLEHAYSKDGGLAVLYGNFAENGCIVKTAGVDDSILKFTG
                     PAKVYESQDDAVEAILGGKVVAGDVVVIRYEGPKGGPGMQEMLYPTSFLKSMGLGKAC
                     ALITDGRFSGGTSGLSIGHVSPEAASGGSIGLIEDGDLIAIDIPNRGIQLQVSDAELA
                     ARREAQDARGDKAWTPKNRERQVSFALRAYASLATSADKGAVRDKSKLGG"
     gene            complement(29189..30118)
                     /gene="ilvE"
                     /locus_tag="ACHOSC_RS04215"
                     /old_locus_tag="pgaptmp_000843"
     CDS             complement(29189..30118)
                     /gene="ilvE"
                     /locus_tag="ACHOSC_RS04215"
                     /old_locus_tag="pgaptmp_000843"
                     /EC_number="2.6.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004084 - branched-chain-amino-acid
                     transaminase activity [Evidence IEA];
                     GO_process: GO:0009081 - branched-chain amino acid
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain-amino-acid transaminase"
                     /protein_id="HGU4546831.1"
                     /translation="MTTKKADYIWFNGEMVRWEDAKVHVMSHALHYGTSVFEGIRCYD
                     SHKGPVVFRHREHMQRLHDSAKIYRFPVSQSIDELMEACRDVIRKNNLTSAYIRPLIF
                     VGDVGMGVNPPAGYSTDVIIAAFPWGAYLGAEALEQGIDAMVSSWNRAAPNTIPTAAK
                     AGGNYLSSLLVGSEARRHGYQEGIALDVNGYISEGAGENLFEVKDGVLFTPPFTSSAL
                     PGITRDAIIKLAKELGIEVREQVLSRESLYLADEVFMSGTAAEITPVRSVDGIQVGEG
                     RCGPVTKRIQQAFFGLFTGETEDKWGWLDQVNQ"
     gene            complement(30138..30401)
                     /gene="ilvM"
                     /locus_tag="ACHOSC_RS04220"
                     /old_locus_tag="pgaptmp_000844"
     CDS             complement(30138..30401)
                     /gene="ilvM"
                     /locus_tag="ACHOSC_RS04220"
                     /old_locus_tag="pgaptmp_000844"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase 2 small subunit"
                     /protein_id="HGU4546832.1"
                     /translation="MMQHQVNVSARFNPETLERVLRVVRHRGFHVCSMNMAAASDAQN
                     INIELTVASPRSVDLLFSQLNKLVDVAHVAICQSTTTSQQIRA"
     gene            complement(30398..32044)
                     /gene="ilvG"
                     /locus_tag="ACHOSC_RS04225"
                     /old_locus_tag="pgaptmp_000845"
     CDS             complement(30398..32044)
                     /gene="ilvG"
                     /locus_tag="ACHOSC_RS04225"
                     /old_locus_tag="pgaptmp_000845"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001012636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0003984 - acetolactate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0009082 - branched-chain amino acid
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase 2 catalytic subunit"
                     /protein_id="HGU4546833.1"
                     /translation="MNGAQWVVHALRAQGVNTVFGYPGGAIMPVYDALYDGGVEHLLC
                     RHEQGAAMAAIGYARATGKTGVCIATSGPGATNLITGLADALLDSIPVVAITGQVSAP
                     FIGTDAFQEVDVLGLSLACTKHSFLVQSLEELPRIMAEAFDVASSGRPGPVLVDIPKD
                     IQLASGDLEPWFTTVENEVTFPHAEVEQARQMLAKAQKPMLYVGGGVGMAQAVPALRE
                     FLATTKMPATCTLKGLGAVEADYPYYLGMLGMHGTKAANFAVQECDLLIAVGARFDDR
                     VTGKLNTFAPHASVIHMDIDPAEMNKLRQAHVALQGDLNALLPALQQPLNINDWQQYC
                     AQLRDEHTWRYDHPGDAIYAPLLLKQLSDRKPADCVVTTDVGQHQMWAAQHIAHTRPE
                     NFITSSGLGTMGFGLPAAVGAQVARPNDTVVCISGDGSFMMNVQELGTVKRKQLPLKI
                     VLLDNQRLGMVRQWQQLFFQERYSETTLTDNPDFLMLASAFGIPGQHITRKDQVEAAL
                     DTMLNSDGPYLLHVSIDELENVWPLVPPGASNSEMLEKLS"
     gene            complement(32047..32097)
                     /gene="ilvX"
                     /locus_tag="ACHOSC_RS04230"
                     /old_locus_tag="pgaptmp_000846"
     CDS             complement(32047..32097)
                     /gene="ilvX"
                     /locus_tag="ACHOSC_RS04230"
                     /old_locus_tag="pgaptmp_000846"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide IlvX"
                     /protein_id="HGU4546834.1"
                     /translation="MNNSTKFCFSRFRTGN"
     gene            complement(32184..32282)
                     /gene="ilvL"
                     /locus_tag="ACHOSC_RS04235"
                     /old_locus_tag="pgaptmp_000847"
     CDS             complement(32184..32282)
                     /gene="ilvL"
                     /locus_tag="ACHOSC_RS04235"
                     /old_locus_tag="pgaptmp_000847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0031556 - transcriptional attenuation by
                     ribosome [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ilv operon leader peptide"
                     /protein_id="HGU4546835.1"
                     /translation="MTALLRVISLVMISVVVIIIPPCGAALGRGKA"
BASE COUNT         7811 a         8925 c         8204 g         7415 t
ORIGIN      
        1 gtcacaactt ctaataatgg ggtggctaat gggattcgaa cccacgacaa ctggaatcac
       61 aatccagggc tctaccaact gagctatagc caccactaca aatcttgtta cgcggtatta
      121 ctaccaccgc agctcaagcg ccgggactaa atggcgcgcc cgacaggatt cgaacctgag
      181 acctctgcct ccggagggca gcgctctatc cagctgagct acgggcgctt agcgccgttg
      241 cgggggtgga taatacggac ttcccacccc tctgtctagt ccctttttaa ataaattgcg
      301 cgtttggtta cggtttgtgc gttttgctga ttattcctcc agtttatgcg ctttcccgtg
      361 gcgattaagg ccaaaaactt tataaatcgc cgtcaccgcc agcataaaga tgatgccaac
      421 aaacagcgac atacgcgtat cttcattaaa gtacatgccg atcaaaacgc aaatcaggaa
      481 tgccattgtt acgtagttgg cccacgggaa caggattgag cggaacggat ggctggcaat
      541 cgccgcttta tgtgcacgcc gaaaacgcag ctggcttatc aatatcacaa accatggcac
      601 catccccgga agcacgctgg cactgtagac gtagacaaac acacgctgcg gattgggaat
      661 gatgtagttc aggcatgagc caattagcag aatagcaata gatactgcca cacccgcaac
      721 tggtacgccg tgacgggaaa ctttcgccat cgccgccggt aactgacggt ttttcgccag
      781 tgcgtagagc atacgtccgc aactgtacat gccgctgtta cagccagaga gcgcagccgt
      841 cagcaccaca aagttgataa tgcccgccgc tgcggtaata ccgattttgg caaaagtcag
      901 tacgaacggg ctgccgttgc tgccaatttc attccacggg aagatggtga cgataacgaa
      961 aatcgcgcct acgtagaaaa tcaggatccg ccacagcact ttgcctacgg cactgcgcag
     1021 cgtcacctgc ggattcttcg cttcaccggc agtaatgcca atcagctcca cgccctggta
     1081 ggacgccacc acaatacaca gcgcggtcag gaaccctttc cagccacccg caaagaaacc
     1141 gccatgctct gtgagattgc taaaaccaat cgactgcccg ccattgccaa agccaaagaa
     1201 aatcacgccc aggccaatga caatcatcac gataatcgtg gtgactttga tcatcgcgaa
     1261 ccagaactcg atttcgccgt acaaccgcac cgccgccaga ttcgccaacg ccaccagcgc
     1321 cactgcgatc aatgcgggta tccactgcgc catctccggg aaccagaact ggacataaac
     1381 gccaatggcg gtgatttctg agatccccac cgccatccac ataaaccagt aagaccaggc
     1441 ggtgagataa ccaaagaacg ggctcatata acgatgcgca taaacggcga acgaaccggt
     1501 aaccggttcg aggaacaaca tttcgcccat tgaacgcatg atgaaaaaga cgaatagccc
     1561 ggcgatgata taggccaaca atacggatgg cccggcccat ttcaaggtac tggcggcccc
     1621 cataaacagg ccgacgccaa tggtgccccc cagggcgatg agttcgatat gtcgagcttc
     1681 cagcccacgc tgtagctctg gtttgttatc tgccataaat cctcgtgttg tgtttgcatg
     1741 ctttccggtg ttaccggtta tcgttatggg tacatcgagt gttgcgaatg ttttcgtaat
     1801 tcaggagaaa tggcaaataa agcattaaaa atttgaatgc tttgtgtaat aaaaaagcag
     1861 acaggcgacg gagtgaccac tccgtcgctt tacaaagaga ggaaaatcag aggttgccgg
     1921 tgtagtgcca gcgtaaataa cgcagcaaac gaagctgacg cttaatgcgg ctcggctgcg
     1981 aaagcaggcg gtagagccac tccagtccca gcgtttgcca gattttcggt gcgcgtttta
     2041 cgtgaccggt gaaaacatcg taagtcccgc caacgcccat atacagcgca tctggatgca
     2101 ccagacggca gtcgcgcatg aagatctcct gctttggcga tcccatcgca acggtgacga
     2161 tttgcgctcc gctggcatga atgcgttcaa acagcgcctg acgctgctcg ggtttaaaat
     2221 aaccatcctg actgccaacg atattcacat tccactggtt gcgcagttta gcttcagttt
     2281 gcgccagcac ttcaggttta ccgcccacaa gaaataccgg cgtcccttct ttgcctgcgc
     2341 gcgccatcag ctcttcccag agatcagcac cggcaacgcg ggaaacctgc gcctgcgggt
     2401 actttttacg tactgaacgt acaacgctga tgccatccgc atatttaaat tcggcagcgt
     2461 taattagctc cctgacctca gcattatctt caatggtcag cattttttcg gcattaatgg
     2521 caaccagcgt tccctgctta agctgcccgt cagcaaacag ataatcgagg gcgtgctgca
     2581 tatcacgcca accaatcaac tgtaagccac gcagcgtata ggtcggtgcc atggtgttgt
     2641 tattcattgt tatccttcaa cctgcgtccg gagcgatgat tttgtacgtt tatgaatgag
     2701 tccggcgctt tcaaaaagcc agtacaacag ttttgcgatc atcagacatg cgccgaagac
     2761 cacgataaaa aagactacgc gtgagacaaa cgaatccagc ccttcacgtg ccagcacgat
     2821 catattgaag atcgccccaa agcagaaact gtgcaatatc gcagccttat agcgattagg
     2881 ctcgcggttg cccagctcat acagccagtc gaaccatttg atgatcagac caaccacgat
     2941 cgccccgagc gggatgaaca acgcgccgcc catcaccacc agtgagccta taagcgtagg
     3001 cgagatcgcc agtccggagt ggttattcag cacttcccag gtaaagtagt tggctgagtt
     3061 cagcaccata ctcgggcgac ccggccacag ccaggaaggg ataaagacat agaaatcgcg
     3121 gacaattgga gccaggccct ggaagtcgat gttgtcgtag ttctgcaaca gcaacgccag
     3181 attctcccac ggcgagaagg tgtcgcgagt gagatagaga aacgtataga acgcttcatc
     3241 gccgctcaca ttcattccat agcgttttag cgccagccag aacatgccca caatccccag
     3301 cacgcccgcc gccgccagca tccacaacga aatccagccg cgaataatgc caataaacag
     3361 gaagatagcg aatgcgatga tgatattggc gcgagtgccg ccgacaatca tataagtcag
     3421 caagccaaag gcgaccgtgc tgacgaggaa aaacagccac gctttgctat cctggcgcag
     3481 aaagtagacc accagcatcg ccgggatgaa aaagtaaaag aagcgtttta acgccacgcc
     3541 ggagacttca ctggagaaga tctgactgta ggagttaagc cggaacagca aaaagccgtt
     3601 gtgcatgaag aagatgccga cgcttaccag cgcgatgccc atcaggatca cccacgtaag
     3661 attggtctcc acgcggttca tggtaaacag cggacggcgc ggtacatcag caacgcgttt
     3721 gcgtaggcgg gttttgtagg tgacatagta aaccgcgtag aagcagcccg cagaaagcaa
     3781 cgcctgcaac aagatttctg gcggcgcgac accaacatca aagcgaaata ccagcacgct
     3841 ggtcagcggg aagccgaaga aaaaggtgag caaaaacagc aatgaaaaga agacattgaa
     3901 gttaaagcgc acacggcgaa actcaaacca ggtcagcgtg gcaataaaca gcgtgcagag
     3961 cagccagacc acaaacaggc cactgaattg cagcagactc atgcgccctc cccggcggca
     4021 atcgccaacg cccgctgcca gccttgtaga tagttagggc taaagaaggc aatggtgttt
     4081 ttatccaccg acgccaactg gcgctgcgct tcacgcacaa tatcctcgtt gagatcgtcg
     4141 gtagtaaaca gcactggcaa atgctgttcc gtcatatcct gccagaacgg attttcccgg
     4201 ttaagcacac aaggaatgcc cgcctgaatc agtaagcaca gcgtaccaat gccctgctgg
     4261 cgagcaaaaa taaagtaacc aagatcgcac tgacgaagta gcgccagata ggcgtcaaat
     4321 tccagttttt cgctcaaaat ttgcagattt tcttcgctga ataactccag ccccgcctga
     4381 cgaacttcct caatgtacgc ttcgttatta ggcggatatc ccatcggcac caccactttt
     4441 accgtatcgc caaattgctg atgaacggca cgtaaagcgg cgacatgctc attgctgagg
     4501 tcgccggagt tccccaccag aatggtcatt tttccttcac gttgccgatc gttcgccatc
     4561 gtattgagag aagggtccat ccgcgtcggg aagtacagca gttctccccg cacctttggg
     4621 tgcgttttgg caaaaaagct caaatcgccg cgggtggcaa atacacagcc gactcgcttt
     4681 tgcgccaggc gacgtagtgg gtaaaaaagc ttatatctca agccactgga aagctcatac
     4741 aggtctgccc cccagatatg ccagaaaaac tggctgggct taatgccacc actcagcaga
     4801 gccagccaca gtgtgggatt gaactgaccg tggaaaaaaa atcgctgctg acggttagct
     4861 tttgctttcg cgatgaccgc ttccgccagc gattttttcc cagggaaaaa ttgcacagaa
     4921 agcgccggac agctatcact taagccgtcg tccttgccaa caaccataaa ctcgcgcgcg
     4981 tgctcgctcg tcgcagccag cgcgtcattg aaaaaccgca aaacggttcg gttatggtga
     5041 gggatatccg atcccagtac gtgaatcagt acagtcatgc ccgcctacgc cagagtaaaa
     5101 acacgccaca acaaagagaa aaatagacga tataagttgc catatatgcc tgcgccgcgc
     5161 ccagtgcacc atgcgcaggg attagccagt gggcaaatac catcaataaa gtgaactggc
     5221 tgatttccgc cagaatataa aaccgcaatg acgctttggc gatcaccaga taaccaaaga
     5281 cataagcgcc cacttttaac acatcaccca ctaactgcca ggcaaagaga tcgcgcatag
     5341 cggtaaattt attcgacaac agcagccaga tagcaaaatc acgcagcagc cagacggtaa
     5401 aactcgccgc cgccactgcc ggtaagacga atttcagcga tttaaccact tcccgggtga
     5461 tatcgcgctt ttccgttagc cgcgacaacg tgggcagcaa atatacgctg aacgatgccg
     5521 taataaattg caggtaggca tcggaaatac tgctcacccc ttgccagatc cccacttcat
     5581 cccagctata ctgcgccgcc agcagtttac gcatcatgat gtaagcaaca ggcaaggtca
     5641 ccgacgtaat caacgccatg agcgtaaatt tgctcaactg ccctgccaga ccgttatccc
     5701 agctgggttt cagatagctt aacgggatga caccacgttt gatcaacatg atagtggcag
     5761 gaattaccac cagcgcggga atcagcgcca gacccagcaa cgccccttca taaccgccca
     5821 aacggtaact gacgtagtac gcgagaacgc caatcaagct gccgacaatc agcgataacg
     5881 cattacctgc ggcatcgcga aagcctttca tcagcgccag taacaggttg ccccaggcga
     5941 tccccatttg caccagcgcc actaaacgca ccagcccctg atagtcggta ttaccaaaca
     6001 atccctggct gattggcgca gctgccagca caaaaaccag cgccatcagc gtagagaaac
     6061 caagtaccat cgctgatgaa gtgccgacca cgcggcgcag ctgttgcgga ttatcatggt
     6121 actgggcaac gtatttggtt acaccgttaa agatgccagc tccggcaagc acgccgagca
     6181 cggtaatcaa ctggcggaaa tttgccgcca gcccaagccc cgccggacca aatgacacag
     6241 ccagcaactt accgaccagt aacccggcac caatcttgac cagtgtactg gccgccgtcc
     6301 acaaggacgc ttttgccaac gacatatcag gagaagtagt tcaacaaagt cgcaattacc
     6361 gtacgctgat tgacgggcga caggttgtag aacagcggca ggcgcagcag gcgctcgctc
     6421 tctttggtgg tgtagcgatc ttcaccgtgg aactcaccaa agcgttcccc cgcagggcaa
     6481 ccgtgcagcg gaatgtaatg gaacaccgcc atgatttccg cttctttcag aaagttaatc
     6541 aacgcgctcc ggtcatcaat atcccgcagt ttaatgtaga acatatgcgc gttctgcacg
     6601 cagccatcgg gaatcgacgg cagctcgata cgcccggctt tcgccagagg cgctaacgca
     6661 tcgtagtagt tttgccacag cgccagacgt tgctggttga tacgatccgc tgcttccagt
     6721 tgcgcccaca ggtacgcagc ttgcagatcg gacatcaaat agctggagcc aatatcgcgc
     6781 caggtatatt tatcgacctg accacggaag aactggctgc ggttagtgcc cttttcacgg
     6841 atgatctcgg ctcgttcgat taacgcttta tcgttaatca gcgtcgcgcc gccttcacca
     6901 cccgccgtat agtttttggt ttcatggaag ctaaagcagc caatatgacc aatggttccc
     6961 agtgcacgcc ctttgtaagt ggacatcacg ccctgagcgg catcttctac cacaaacaga
     7021 ttatgctttt tcgccaacgc cataatggtg tccatttcgc aggccacacc cgcgtaatgg
     7081 accggcacga taacgcgcgt tttgtcggtg atcgccgctt caatcagcgt ttcgtcgatg
     7141 ttcatggtgt ccgggcgaac atccacaaaa acgatttttg cgccacgcag cacaaaggca
     7201 ttggcggtgg agacaaaggt gtagctcggc atgatcactt catcgccagg ctggatatcg
     7261 agcagcagcg ccgccatctc cagcgaagcg gtgcaggacg gcgtcagtaa cactttggcg
     7321 ctgccaaaac gttgctccag ccactgctgg cagcgacggg taaaaccgcc atcgccacac
     7381 agtttgccgc tacccattgc cgactgcata tagtcgagtt cggttcccac caccggcggt
     7441 gcgttaaatg gaatcatgtg atcacctgta taaccagtac gcggtgcttt ctacattcgc
     7501 accactttgt atgtatcgtt taagcgcggc ggtgttgccc atttgggtcg ccacccgcaa
     7561 agttgtttta ccgcgagcat acgcccagtt tagcgccgtt tgcatcagct cagcacctac
     7621 accgcgtcca gccagcaggc caattcgcgc atctgtcgca ttgagttccc gtaaagagac
     7681 ataaccgcga ataccgccgg acgccgcacg taaaatcaga cattgatgat caaaggtgcc
     7741 gcgcacggca ttttcaatcc actgtgcata aaagcgtctg ctggcgtcag gcgcatacca
     7801 cggcgcacga aaacggcttt gcgcaaatgc ggcgctggct aactgacgta atgcgggaat
     7861 atcggtctct tgtgccacta cagcaccact atcactgaca ttgttcacgg gtagtgccaa
     7921 atcaacttca ccttctacca gggagaatcc cagctgttgc agggcatcca gttcacccgt
     7981 atttgatgcc gcaattttgg cctgcacccg tgaccacggc gctaacacgt ctggcgtcag
     8041 gagcggtgct tcagacgtaa tgcgcacgat ggcgctgtta acaccaaaga aggcgttttc
     8101 ccaggttagt ggctcaatac tggcgcggac gggcacgaag taactccagc agatattggc
     8161 cgtagccagt tttcgctaat gaactggcag cacgcttcac accctcgtca tcgagccagc
     8221 cgttacgcca ggcgatctct tccaggcagg caatcttaaa gccctggcgt ttttctactg
     8281 tctgcacaaa ggtactggct tcaatcaggc tgtcgtgagt gccagtgtcc agccaggcaa
     8341 atccgcgccc gagcagttca acggtcaggt tgcccgcctc gaggtacatc tggttgatgg
     8401 aggtaatctc cagttcacca cgctccgacg gcttcacctg ctttgcgtac tccacgactt
     8461 tactgtcgta gaaataaagc ccggtcaccg cccagtttga cttcggctgt tttggctttt
     8521 cttccagcga gatagcgcgg aaattgtcgt caaactccac cacgccaaag cgttccgggt
     8581 ccatcacctg atagccaaat accgtcgccc cttccgtgcg cgccgcaaca tgacgcagct
     8641 tcggactgaa gccctgaccg aagaagatgt tatcgcccag caccagacaa gaaggttcac
     8701 cgttgaggaa ggtttcaccg atgataaagg cctgcgccag accgtccggg ctgggctgtt
     8761 cggcgtattc cagctggata ccgaactcac taccatcgcc cagcaggcgc tggaaataac
     8821 ctttatcttc cggcgtagtg atgatgagaa tttcgcggat accggccaac atcagcaccg
     8881 acagcggata gtaaatcatt ggcttatcgt aaatcggcaa cagttgcttc gatacgccgc
     8941 gcgtaatcgg atgcaatcgg gtgccggaac cgcccgccag gataatacct ttcatgtttg
     9001 cctccgctga aaattagcct ttcagaccta aacgctcgcc ctgatagctg ccgtcctgca
     9061 cctgcttcca ccagctttca ttagccagat accactgcac cgttttacgc attccacttt
     9121 caaaggtttc ctgtggcagc caaccaagtt cacgggcaat tttcgaagca tcaatggcat
     9181 aacgcagatc atgccccgga cggtcagcga caaaggtgat caagtcacga tagtgcgcca
     9241 cgccgtgcgg cttgttcgga gccagttctt ccagcagctc gcaaatggtt tccacaacat
     9301 cgagattctt acgctcgttg tgaccaccaa tattataggt ttcaccgact ttcccggtgg
     9361 tcgccacgca atacagcgcg cgggcgtgat cttccacata cagccagtca cggatttgct
     9421 gcccgttgcc atataccggc agcggtttac ccgccagcgc gttgaggatc atcagcggga
     9481 tcagtttttc cggaaagtgg taaggaccgt agttattcga gcagttggtg atcagcgtcg
     9541 gcagaccgta ggtccgcagc caggcgcgca ccagatggtc gctactggct tttgacgcgg
     9601 aataagggct gctcggcgca tacggcgtgg tttcggtgaa gaaatcatcc gtcgagtgca
     9661 ggtcaccata tacttcgtcg gtggagatat gatgaaaacg gaacgctgat tttttatctt
     9721 ccgtcagcgt attccagtaa gcccgcgccg cttcaagcaa tgtataagtc ccgacaatgt
     9781 tggtttcaat aaacgctgcc gggccgtcaa tagaacggtc aacatggctt tcggctgcca
     9841 gatgcatgac acagtctggc tgatgctcag tgaatacgcg tgccagttct gcccgatcgc
     9901 agatatcaac tttctcaaag gcaaagtgct cgctttgcgc gaccggtgcc agcgacatca
     9961 ggtttccggc gtaggtcagc ttatcgacca ctaccaccgc atcgctcgtt tcgttgatga
    10021 tataacgcac cagcgccgag ccaataaacc cggcaccacc tgttatcaga atttttctca
    10081 tcagcgccag actcctttgg catcgacgac atactgctga tggacattgt cgccattgat
    10141 aactttgaac tgactatgat cgaccagcat caccagcacg tctgccgttg ccagcgcctc
    10201 gtcaagctgc gccagagtac aaagcccggt cagttttttc ggcaactggt ggatgttagg
    10261 ctcaacaacc agcgtttcgc cgctgtgcca ctgggcgatc agttcagcga tttccatcgc
    10321 cgggctttcg cgcaggtcat caatattcgg tttaaacgcc agaccaaagc aggcgatttt
    10381 cagttcactg gcgcgtttat cggtagcagc aaggcagtcg gcaaccgccg ctttcacctg
    10441 atcgataacc cagaacggtt tgtgatcgtt aacttcgcgc gcggtacgga taagccgcgc
    10501 ctgctggggg ttctgtgcca cgataaacca cggatcaaca gcaatgcagt gaccgcccac
    10561 gccagggcca ggctgaagaa tattaacgcg agggtgacga ttcgccaggc gaatcagttc
    10621 ccagacgtta atcccctgat cggcacaaat cagcgacaat tcattagcaa aagcgatatt
    10681 cacatcgcgg aagctgtttt cggtgagctt acacatttcc gccgtccgcg agttagtgac
    10741 gacacactca ccttcgagga aaattttgta cagttcgctg gcgcgggtcg aacaaaccgg
    10801 cgtcatacca ccaatcacgc gatcgttttt aatcagctcg accataacct ggcccggtaa
    10861 cacgcgttcc gggcagtaag caatgttgac gtccgcctgc tcgcccacct gctgtgggaa
    10921 agtgagatcc ggacgcatct ctgctaacca ttctgccatc ttctcggttg accccaccgg
    10981 cgaggtggat tcaaggatca ccagcgcgcc ttttttcagc actggcgcaa tggagcgagc
    11041 agccgattca acgtaggtca tatctggctc atgatcgcct ttaaacggcg tgggtacagc
    11101 aatcagccag gcatccgctt caactggcgt cgtgctcgct cgtaaaaaac cgccttctac
    11161 ggcagttttt actacgctcg ccaaatcagg ctcgacgata tggatttcgc cacgattgat
    11221 ggtatcaacc gcgtgttggt tgatatcgac accaatcacc tgtttttgcc gtgaggcaaa
    11281 cgctgctgct gtaggcagcc cgatgtaacc cagtccgata acagaaatgg tcgcaaaact
    11341 catagtgata tccgattatt ttttaacgct tccagaatgc gagagcatgc ctgaccatca
    11401 ccatacgggt tatgggcgcg gctcatagtt tgatattcgt tttcgtcttt taaaagacgc
    11461 gtcacttcct cgacaattcg ctgcttatcc gtgcctacca gacgcaccgt acccgccgtc
    11521 accgcttccg gacgctcagt ggtatcgcgc atcaccagca ccggtttccc cagcgaaggc
    11581 gcttcttcct gaatgccgcc tgagtcggtc aaaatcagcc aggcgtggtt catcagccag
    11641 acaaacggta aatactcctg gggatcgatc agaatgacat ttttcacatg ccccagaatg
    11701 cgattgaccg gctctctgac gttcgggttg agatgcaccg gatagacaat ctggatgtcc
    11761 tggtgcgtgg tggcgatgtc tgccagcgcg tggcagattt cttcaaagcc acgaccgaaa
    11821 ctttcacgcc tgtgaccggt caccagaatc atctttttat cggggtcgat aaacgggtaa
    11881 tttgccgcca gttctgaacg cagcgtgtcg ctgctcatca cctgatcacg cacccataac
    11941 agtgcatcaa tgactgtatt accagtaatg aagattcggc tatccgcaac gttttcacgc
    12001 agcaagtttt gccgggaagt ttcggttgga gagaagtgat acatcgccag atgcccggtc
    12061 aatgtacggt tagcctcttc cggccacggc gaatagagat cgcccgtgcg taaaccagcc
    12121 tcaacgtgac caacaggaat acgctgataa aacgccgcca ggctggttgc cagcgtcgtt
    12181 gttgtgtcgc cgtgaaccag cacgacgtct ggtttgaact cggcaagaat aggttttagc
    12241 ccttccagaa tccgacaggt tatctctgtc aggccctgtc ctggctgcat tatgttgaga
    12301 tcgtagtcag gtacaatgga aaagagtttc agcacctgat cgagcatctc ccgatgctgc
    12361 gcagtgacgc aaactttagc ctcaaaaaaa ggatcttttg ccaacgcatg caccaacgga
    12421 gccatcttga tggcttccgg gcgcgtacca aatacagtca gtactttcac atcgattctc
    12481 ttcgaataag cggcgagcgc ctttgcgctc gccgcagcag tgttgctatt tcgagcaacg
    12541 gcgggttaat gcgacaccag ccccgatcag ccccccgaca atgccccaca taatcatcag
    12601 gaaggcacga cgtgggctat cgcgttttac cggttcttcc ggcgtacgca aatagcgata
    12661 ggtctgaaaa cgcggatcca gagttggacc aacattcagg gtgtttaaca tggcccgatt
    12721 ctgatcatag tcgagatcaa aggccggacc gacggcctgt aaattttcca gtcgagcctg
    12781 gagcattgga cgcccaagca ggaacatttc tgaatcaggt aattcctcgg caggcacatc
    12841 tgtcgcactg cgcgaaatat tatgctgctc agcaattttc agcgcctgct caatgctgtt
    12901 catccggcgg tcgtagatgg ctttcgccac ctcttcctga cgcttcacct gagctttcat
    12961 ctggatggtg cgtgccgccc atgcgccttt cagctcctca ttcagatggc tggctgcacg
    13021 ctggctggca aaagcaacat actgacgtaa caggttatta gcgtcaggcg cggtttcggc
    13081 aataagcttc acgctgtcat tgaccgcgcg ggtaaagtct ccggggataa actggatgtt
    13141 gttaatcatt tcatccagca acgccgcatc ggctttgctg ttgcccacca tccgctgctt
    13201 gtaatagtcg gtttgcagcc agaactctct gcgggtatcc cacgaggcca gttgcataac
    13261 aaactcttta taggcttcgt ccatgaccga tggttggtcg gcagaagcca tgtttgaacg
    13321 gacatccagg ttacgcaaaa attgctgctg cgagtaatat ccccccagca tattcaccgt
    13381 tggacgatcg gtaatcgccg tcgcgctcca ctcttgtcga gcaaaaaaag tatacgccag
    13441 cgcgattaac gcaaacgcca gccccatgcc aataatccat agcttcccag cccacaaggt
    13501 acgaaacaac ccacgaatat ccagttcatt ttcagcgtct tcggccggtt tcccaggcat
    13561 tggttgtgtc atcacatcct catttatttg gttaaattgg ggctgccacc acgatttcta
    13621 cgcagtctgc gttttacgcg cttaataaag cgagcaactt tccaggcacg cttaatgcaa
    13681 tatccataga ggaagaatgc tagcaaaaag agcaccagca tgacccactc cgggacaaaa
    13741 tgagaatatt ctgccagcac gccaatggaa gcgagcagtg ctgcggcaag ggtaatcagc
    13801 acaaacgcct gacgggaagt aaacccggca cgcatgatca aatggtgaat atgctgacgg
    13861 tcaggagaga atgggctcat gcctttacgc aggcgacggt acataatcgc caccatatcc
    13921 attagcggaa tggcgattat ccacaaagcg gtaaccgggc tgatgggatg ggttttgccc
    13981 tgggtcgttt cgagcaggat ccagataacg gtaaaaccaa tcagcgtact gcccgcatca
    14041 cccataaaga ctttgtagcg gcgacccagg ataccaaggt taagcatgat gtatggcagg
    14101 atggcggcga tcatcgcaaa gcaccagatt gcgaggctgg tttgcccgtc gaaccacaaa
    14161 atcataccga ttgctgcaaa cgagacgcag gacaacccgc ccagcaagcc atcaatgcca
    14221 tcaaccatgt tgaacgcatt aatggccgcc cagacggcaa atagcgtcag gaagtaacca
    14281 aacggtccga gcaccatctc ccaggagcca aagatataac ccaggctact gagataaagt
    14341 ttgccgaaca ccatcataac aatgccaaca gcggcctgta tggtggcacg gatttttacg
    14401 ctgatatcaa aacggtcatc cagcgcgcca atgaaaacaa gcacaccggc acaagcgaga
    14461 tagagagatg catgcggaat atagtaatcg acaattccga acgtgaagca aatccctgcg
    14521 taaaccgaaa tccccccaac gagaggtatc aatccctggt gacgtttgcg gaagtttggt
    14581 ttatccacta aaccgacttt ttttgccact ttacgggcaa aaaacagaaa cagtgtcgtg
    14641 aataaaaaaa tactgatgag atcagtactc actgtcagta aattcacaat gcatgctctc
    14701 agagaaaatt attagcagaa gtataaccac gaagaccttt attcagaagg gaaagtccgt
    14761 tccgaaccca ccagtcctgt gcaaaaatta accatttgat atgcaaattg cataacatgc
    14821 cagcaaattc caggaatttt cctaacatcg ccagcgcggc attaagatta cagatataaa
    14881 agcaaaacgc cacgtaaaca cgtggcgttt ttggcataag acaaatttat gagcgtttca
    14941 tcatttcgaa gaaatcgtca ttggtcttag tcatggccag tttattaatg aggaattcca
    15001 ttgcatcgat ttcgcccatc gggtgaatga ttttgcgcag gatccacatt ttctgcagtt
    15061 cttcctgagt cgtgagcagc tcttctttac gggtaccgga acggttgtag tcgatagccg
    15121 ggaagacgcg tttttcagcg atcttacgag agaggtgcag ttccatgttg cctgtacctt
    15181 taaactcttc gtaaataact tcgtccattt tagaaccggt atcgataagc gccgtcgcga
    15241 taatggtcag gctgccgccc tcttccacgt tacgcgccgc accgaagaag cgtttcggac
    15301 gatgaagggc attggcgtcc acaccaccgg tcaacacttt acctgacgcc ggaacaacag
    15361 tgttgtaagc gcgcgccaga cgagtgatgg agtcgagcag aatgataacg tctttcttgt
    15421 gctcaaccag gcgtttggcc ttctcgatca ccatttccgc aacctgaacg tggcgagatg
    15481 cgggttcgtc aaaggtagaa gcaacaactt caccttttac cagacgctgc atctcggtta
    15541 cttcttccgg acgttcgtcg atcagcagaa ccatcagcac acaatccggg tggttgtaag
    15601 caatgctctg agcaatgttc tgcagcagca tagttttacc ggctttcggc ggtgccacaa
    15661 tcagaccacg ctgaccacga ccgataggtg atgccagatc cagtacgcga gcggttaaat
    15721 cttcagtaga accgttacca cgttccatac gcagacgaga gtttgcgtgc agcggggtta
    15781 agttctcaaa gaggattttg ttgcgggcgt tttcaggttt gtcgaagtta acttcgttaa
    15841 ctttcagcag cgcaaaatag cgttcacctt ctttcggcgg gcgaatctta ccagagatgg
    15901 tatcaccagt gcggaggttg aaacggcgga tttggctagg ggaaacgtag atgtcatcag
    15961 gaccggcgag gtaggagctg tctgcggaac ggaggaaacc aaatccatcc tgcaatatct
    16021 ccagtacgcc atcaccaaag atatcttcgc cactctttgc gtgctgcttc aggatggcaa
    16081 aaataatgtc ctgcttacgc atacgagcca ggttttccag ccccatattt tcgccgagag
    16141 tgatcagctc agaaaccggc gtattcttta attcggtaag attcatagtg gtgtgagttc
    16201 ttaaacttgg ggtaaatctc gaacttaatg ttgtgaatgg tatggcagga tcatccatgc
    16261 ctgtttacgg tcatcgactc atgtcttttc gctgcctggt cacaggagag tacgcagaac
    16321 tgaaacgaca agacggattg agtgacgagc cagaaatctg ttcacttcgc atttaagata
    16381 aaacgggaag cgttggaaat tacaagattc aaacttaata aggtatgttt aatacgaagt
    16441 caacactaag ttagcatgac tcacgccggg cgtccagttt ttagcgacgg ggcacccgaa
    16501 catgaaattc ccttacgcca ggttagcgtc gaggaactct ttcaactgac ctttagacag
    16561 tgcacccact ttggttgccg ccacttcacc gtttttgaac agcagcagag tcgggatacc
    16621 acggatgcca tatttcggcg cagtgccagg gttttgatcg atgttcagtt ttgcaacggt
    16681 cagtttgccc tgatattcgt cagcgatttc atccagaatc ggggcgatca ttttgcacgg
    16741 accgcaccac tctgcccaga aatcgacgag gatcgccccg tccgctttga gtacatccgt
    16801 gtcaaaactg tcgtcagtca ggtgaataat tttatcgctc atatataact ccacaggaat
    16861 aagcctggcg tgttggtttc gttgttggtg taacattaac caactaaagg ttgactttat
    16921 ttcaccggat acgctttcgt aaagcaatag taagctgata ttctaccaca ctatgagcaa
    16981 aacacattta acagaacaga agttttccga cttcgccctg catccgaagg ttgtagaagc
    17041 ccttgaaaaa aaagggtttc ataactgtac gcccattcag gcactggccc ttccgctgac
    17101 gctggcgggt cgtgacgtag ccgggcaggc gcaaaccggt accgggaaaa cgatggcgtt
    17161 tcttacgtca acgtttcatt atcttctctc tcatcctgcg attgccgatc gcaaggtgaa
    17221 tcaaccacgt gccttaatta tggcaccgac gcgtgaactt gccgtgcaga tccatgccga
    17281 cgcagaaccg ctggcggaag ctaccggcct gaagctgggt ctggcttacg gtggtgatgg
    17341 ctacgacaaa cagctgaaag tgctggaaag cggcgttgac attctgattg gcactacggg
    17401 gcgtttaatt gactacgcca agcagaacca cattaacctc ggtgccattc aggtggtggt
    17461 actggacgaa gccgatcgca tgtacgatct gggctttatt aaagatatcc gctggctgtt
    17521 ccgccgtatg ccgcctgcaa accagcgcct caacatgctg ttctccgcca cgctttcgta
    17581 ccgggtacgt gaactggcgt tcgagcagat gaacaatgcc gaatatattg aagtggaacc
    17641 ggaacagaaa acgggccacc gtattaaaga agagcttttc tacccttcta acgaagaaaa
    17701 aatgcgtttg ctgcaaacgc tgatcgaaga agagtggcca gacagagcga ttattttcgc
    17761 caataccaaa taccgttgtg aagagatctg gggccacctg gcggcagatg gtcatcgtgt
    17821 cggtttgttg acaggcgatg tcgcgcagaa aaaacgtctg cgtattcttg atgaatttac
    17881 ccgtggcgat ctggatattc tggttgccac cgacgttgcc gcgcgtggtt tgcatattcc
    17941 ggcagtgacg cacgtcttta actacgattt acccgatgac tgtgaagatt acgttcaccg
    18001 tattggtcgt acaggtcgcg caggcgcaag cggtcactct atcagcctgg cgtgtgaaga
    18061 gtatgcattg aatttgcctg ctattgagac ctatattggt cactcaattc cggtaagcaa
    18121 atacaatccg gacgcattga tgaccgatct gccaaaaccg ctgcgcctca cgcgcccacg
    18181 tacaggcaat ggtccgcgtc gtactggcgc tccgcgtaat cgtcgtcgtt caggttaaat
    18241 aaaatgtcat actcaaaatc attcaaactg tatcaagatg gcaagagaat gaatcccgat
    18301 gagcatactg tagtaagtga ttcaggtgaa agaatgcagc caacacagag acagattgaa
    18361 ggatgaagag tatgggttcc acctcgtcgc tgtatgcagc cattgatctc ggttcgaata
    18421 gttttcatat gctggttgtg cgcgaggtgg ctggaagcat ccagacgctg acgcgaataa
    18481 aacgcaaagt gcgtctggct gctggcctga atagcgaaaa tgccctgtct aatgaagcaa
    18541 tggagcgcgg ttggcaatgt ctgcgcctgt ttgctgaacg tctgcaagat atccctccct
    18601 cgcaaattcg cgttgtcgct acggcgacat tacgtcttgc cgtcaatgcg ggtgatttta
    18661 ttgccaaagc gcaggaaatc ctcggttgtc cggtacaggt gatcagcggt gaagaggaag
    18721 cacgtctcat ttatcagggc gttgctcaca caactggcgg tgccgatcag cgcctggtgg
    18781 tggatatagg cggtgccagt actgaactgg taaccggcac gggtgcacaa accacctcgt
    18841 tgttcagcct gtcgatgggc tgcgtcacct ggctggaacg ctattttgcc gatcgtaatc
    18901 tggggcagga aaattttgat gctgcagaaa aagcggcacg cgaagtgtta cgtccggttg
    18961 ccgatgaatt acggtatcac ggctggaaag tgtgcgttgg tgcttccggc accgtgcagg
    19021 cgttacagga aatcatgatg gcacagggga tggatgaacg cattaccctg gaaaagttgc
    19081 agcaattgaa acagcgagcc attcattgcg gtcggctgga ggaactggag attgacgggc
    19141 tgacgctgga acgtgcgtta gtgttcccga gtggtctggc gatcctgatc gccattttta
    19201 ccgaactgaa tattcagtgt atgaccctgg cgggcggtgc gctgcgtgaa ggcctggtct
    19261 acggcatgtt gcatctggcc gtcgagcagg atattcgcag ccgtacgctg cgtaatattc
    19321 agcgccgctt tatgatcgat attgatcagg cgcagcgcgt agccaaagtt gcggctaact
    19381 tcttcgatca ggtggaaaag gaatggcatc ttgaagcaat aagccgcgat ttgctcatca
    19441 gcgcctgtca gcttcatgaa atcggcctga gcgttgactt taaacaagcg ccgcaacacg
    19501 ctgcttatct ggtgcgtaat ctggatcttc ccggttttac ccccgcacag aaaaaattgc
    19561 tggcgacgct actgctcaac cagactaatc cggtcgatct ctcatcgctg catcagcaaa
    19621 atgccgtacc tccgcgcgtc gcagaacaac tctgccgttt actccgcctg gccatcattt
    19681 ttgccagccg tcgccgtgac gatctcgtgc cagagatgac attacaggct aaccatgaac
    19741 tgttgacctt gacgcttccg caaggttggc taactcaaca tccgctgggt aaagagatta
    19801 ttgctcagga aagccagtgg cagagctatg tccactggcc gctggaagtg cattaatgag
    19861 taagtgccgg atgcgatgct gacgcatctt ttccggcctt gattatttcc ctcgttttgc
    19921 cgccatcatc gctttcagat tcgccagatg gctttgccct ttctgcatcc gttcttcggc
    19981 gctgaccact ttgcgctcct gttcccaaat cagatcatcc tgcggcagct ccagcaaaaa
    20041 gcggctcggc tccgggcgca ccagctcgcc gtactgacgg cgttctttac acagcgtaaa
    20101 ggtcaattcc ttctgggcgc gggtaatgcc gacataggcc agccgccgct cctcatcgat
    20161 attatcttca tcgatgctgc tctggtgcgg caaaaaccct tcttccatac cgaccatgta
    20221 gacataagga aactccagcc cttttgacgc gtgcagagtc atcagttgca cctgatccag
    20281 ctcttcttca ctctcaccac gctccatcat gtcgcgcaaa gtaaagcgcg tcaccacctg
    20341 ggtgagcgtc atcggctcat ccagttcgct gccttccagc atctccgtca tccagctaaa
    20401 cagttggttg acgttcttca tgcgcatttc ggcggctttc gggctgggcg atgtttcgta
    20461 cagccaggat tcataatcca tgccgtggat cagatcgcgc accgcggcaa tcggctcccg
    20521 ctccgccaga cgctggattt ctgccaacca gtgagtaaag cgggtcaatg cttcataacc
    20581 acgtccgcta agcgtctgac tcaggcccat atcaaagctg gcggtaaaca tgcttttatt
    20641 gcgcgtcatc gcccactcac ccagcttttt cagcgtcgcc gggccaatct ctctcttcgg
    20701 cgtgttaacg atacgcagaa atgcgctgtc atcgtccggg ttagtcagca cgcgcagata
    20761 agccagcaag tccttgattt caggacgaga gaaaaacgac gtaccaccag aaattttgta
    20821 cgggatgcgg ttttgcatca ggaatttttc aaacactcgc gactgatggt taccgcgata
    20881 aagaatagcg taatctttgt actgcgtttt attgacgaag tgatgcgcaa tcagttcacc
    20941 agtgacgcgc tcggcttcat gttcttcgtt attcgcgctt aaaactttaa gctctgtgcc
    21001 gtaacccagt tcggagaaca gacgcttctc aaagacgtgc gggttattgg cgatcaggat
    21061 gttcgccgct ttcaaaatac gtccggaaga gcggtagttc tgctcaagct taatcacctt
    21121 cagcgccgga aaatcctgac tcagcagcac caggttttgt ggacgtgcgc cgcgccagga
    21181 gtagatcgac tggtcgtcat cgcccaccac ggtaaagcgc gcgcggctgc ccaccagcag
    21241 tttcaccagt tcatactggc tggtgttggt gtcctgatac tcatccacca gcagatagcg
    21301 aattttgttc tgccagcgct cgcggacttc ttcattgcgt tgcagcagca acgtcggcaa
    21361 taaaatcaga tcatcgaagt cgagaacatt gcacgctttc aggtgtgcat catacagccc
    21421 ataacaatgg gcaaaaatac gatcccgctc gccctttgcc tctgccgccg cctgcgccgg
    21481 agttttgaga tcgttcttcc agttggagat ggtcgaaatc agctgttgca ggagaacttt
    21541 gtcatcttca atcagcccct cggtcaactc tttaagcaaa gcaagctgat cggtatcgtc
    21601 aaacagcgaa aagttcgcct tcatcccaag cgccgcatac tcgcgtttga tgatatccaa
    21661 ccccagcgtg tgaaaagtgg agatcatcaa cccgcgcgcc tctttgcgac ccagcgtctg
    21721 cccgacacgc tctttcatct cgcgcgccgc cttattggta aaggtcaccg ccgcgatatg
    21781 tcgcgcctga taaccgcaac cgcggatcag atgggcgatt ttattggtga tcacacgagt
    21841 tttaccggaa cccgcgcccg ccagcaccag gcaggggccg gtaacgaatt cgacagcttg
    21901 ttgttggccg gggtttagac gcataggtgt attgctcaat cttcgaacgg ggggaggatt
    21961 gtagcagaat cgcatttgaa tgtgaccgcg tgtaagccgc taaccggttg gctgcggatg
    22021 tttaaatcag agagagaaga ccgaaatcgg agttcatcgt gaatccatta gacttagaaa
    22081 atatcgggtc gtaacgttgt gttattactt tttcagagtt tactaaggag aggtttactc
    22141 gtggcgatta atttcagccc taaggttggt gaaatacagg aatgcaactt tggtaactat
    22201 cctgttatcc agaatggaca gttcagcacc acgtattacg atggccgcat tcctcctgaa
    22261 atgataaaaa acaggttagt tgtcgtgctc aacggaaaaa ttaatggcaa cgcatgtatc
    22321 gtcgttccat tatcaaccac tcgcgatcat gacaagttaa atcgtggaat gcatgttgaa
    22381 atagccagta atgttatcaa tggcctgcaa ttctttgatc agcagattcg ttgggccaaa
    22441 gccgacctgg tacaacaagt cagtcgaaac cgattaaata gagcaaggac ttacagaggg
    22501 tatcttaatc aatgtttgcc acatgagttg gttgcagaca tacagagggc ggtcatcaag
    22561 tcaatcaatg ccatatcgct cataaattga caataaaata accatccccg ataatgactt
    22621 tgtagcccat agaggcccta agttttctcc ccttgaggga cctgcgaagc ccggattttc
    22681 cgggcttttg ccatatatgt accagcccct ccctgactga caattacgtt agcatctgcg
    22741 gatcatgcta aaatcgccgc ctgacaatta ttcactcaag gcacgatcat ggcaaaaaca
    22801 gcagcagcac tgcatatcct tgtaaaagaa gagaaactgg ctctggatct tctcgagcag
    22861 attaagaacg gtgccgattt cggcaagctg gcgaagaaac actctatttg cccgtcaggc
    22921 aaacgcggcg gtgatttagg tgaattccgc cagggtcaga tggttccggc gttcgataaa
    22981 gtggttttct cttgtccggt actggagccg accggcccgc tgcacaccca gttcggatat
    23041 cacatcatta aggtgctgta ccgcaactaa tagcaaggcc ttctccagga gaaggccttg
    23101 agtgttttct ccctctccct gtgggagagg gtcggggtga gggcatcagc gcgcacttaa
    23161 cccgcaacag caatacgttt catatctgtc atatagccgc gcagtttctt acctacctgc
    23221 tcaatcgcat ggctgcgaat cgcttcgttc acatcacgca gttgcgcgtt atctaccgcg
    23281 ccttccggaa tagctttacc caagtcgccc ggttgcagct ctgccataaa cggtttcagc
    23341 aacggcacac aagcgtaaga gaacagatag ttaccgtact cagcggtatc agagataacc
    23401 acgttcattt cgtacagacg cttacgggcg atggtgttgg caatcagcgg cagctcgtgc
    23461 agtgattcat aatatgcaga ctcttcaatg atgccggaat cgaccatggt ttcgaacgcc
    23521 agttcaacgc ccgcttttac catcgcgatc atcagtacgc ctttatcgaa gtactcctgc
    23581 tcgccgattt tgccttcata ctgcggcgcg gtttcaaacg cggttttgcc ggtctcttca
    23641 cgccaggtca gcagtttctt atcatcgttg gcccagtccg ccatcatacc ggaagagaat
    23701 tcgccggaga tgatgtcgtc catatgtttc tggaacagcg gtgccatgat ctctttcagc
    23761 tgttctgaca gcgcataagc acgcagtttc gccgggttag agagacggtc catcatcagg
    23821 gtgatgccgc cctgcttcag cgcttcggtg atggtttccc aaccgaactg aattagtttt
    23881 tctgcgtatg ccggatcggt gccctcttcc accagcttgt cgaagcacag cagcgagcca
    23941 gcctgcaaca taccgcacag gatggtttgc tcacccatca ggtcagattt cacttccgca
    24001 acgaaggacg attccagcac gcccgcacgg tgaccaccgg ttgctgccgc ccatgcttta
    24061 gcgatcgcca taccttcgcc tttcggatcg ttttccgggt gaacggcaat cagcgtcggt
    24121 acgccgaatc cacgtttgta ctcttcacgc acttcggtgc cagggcattt cggcgcaacc
    24181 atgactacgg tgatatcttt acggatctgc tcgcccactt cgacgatgtt gaaaccgtgc
    24241 gagtaaccca gcgccgcgcc gtctttcatc agtggctgta cggtgcgcac tacatcagag
    24301 tgctgcttgt ccggcgtcag gttaaccacc agatccgcct gcgggatcag ttcttcgtaa
    24361 gtaccaactt taaaaccgtt ttcggtcgct ttacgccagg acgcgcgctt ctcggcaatc
    24421 gcttctttac gcagagcgta ggagatatcc agaccagaat cacgcatgtt caggccctgg
    24481 ttcagaccct gtgcgccaca gccgacgatg actacttttt taccctgaag gtagctcgcg
    24541 ccatcggcga attcatcgcg ccccataaag cgacatttgc ccagctgtgc cagctgctga
    24601 cgcagattca gtgtattgaa gtagttagcc atggtgattc ctcgtgatgt tgtgcttctt
    24661 attgttcggt tcgcgtttgc gagattgaat tcactatatg acaggaaatt tattgcggaa
    24721 attgatatat tcacaacgtc acattgcaat ttttgcaacg tcaacatcga gggctgcgcc
    24781 attggattta cgcgatctga aaaccttcct gcatctggcg gaaagccgcc attttggccg
    24841 cagcgcgcgg gcgatgcacg ttagcccatc cacgctctca cggcagattc agcgcctgga
    24901 agaagatctt ggtcagccgc tatttgtgcg cgataaccgc acggtaacgc tgacggaagc
    24961 gggcgaagag ctgcgcgttt tcgcccagca aacgctgttg cagtatcagc aattgcgcca
    25021 caccatcgat cagcaagggc cgtcgctctc tggcgaatta catatcttct gctcggtgac
    25081 cgctgcctac agccatctgc cgccgattct ggatcgcttc cgcgcggaac acccgtcggt
    25141 ggagatcaaa ctcaccactg gtgatgcggc agatgcaatg gaaaaagtgg tcacgggaga
    25201 agcggatctg gcgattgccg ggaaaccgga gacgctaccc ggcgctgtgg cgttttcgat
    25261 gctggagaat ctggcagtag tgctgattgc gcccgcgctg ccctgcccgg tgcgtaacca
    25321 ggtttcggca gagaagccgg actggtctac agtgccgttt atcatggccg atcagggacc
    25381 ggtgcgtcgc cgcattgaac tgtggtttcg acgcaataaa atcagcaacc cgatgattta
    25441 cgccacggtt ggcgggcatg aagcgatggt atcgatggtg gcgctcggct gtggtgtggc
    25501 gctgttgccg gaagtagtgc tggaaaacag ccccgaaccg gtgcgtaacc gcgtgatgat
    25561 tttagagcgc agcgatgaaa aaacgccgtt tgaactgggc gtgtgtgcac aaaaaaagcg
    25621 gctgcatgag ccgctgattg aggcgttctg gaggattttg ccgaaccaca aatgacgttg
    25681 tcgcgcgggt aggcctgata agcgaagcgc tatcaggcat ttttccctaa cccgccaaaa
    25741 agaacctgaa cgccgggtta ttggtttcgt cgtggcaatc gtagcccagc tcattcagcc
    25801 gggtttcgaa atccggttca tggtcgccaa gttcgaacgc cgccagtacg cgcccgtagt
    25861 cggtgccatg gctgcgatag tggaacaaag aaatgttcca gtgcgtaccc agcgtgttga
    25921 ggaagcgcag cagcgcgccc ggtgattccg ggaattcgaa gctgtagagg cgttcctgca
    25981 acggatgtga cggacgcccg ccgaccatat agcgcacatg caacttcgcc atttcgtcgt
    26041 cggagagatc caccacgctg tagccgccgt cgttgagcat ctgcaaaatt tctttgcgct
    26101 cttcgaggcc gcggctcagg cgcacgccga caaagatgca ggcgtttttg gcatcggcaa
    26161 aacggtagtt gaactcggtg accgatcgcc cgccaagcag ttggcagaat ttgaggaagc
    26221 tgcctttttc ttccggaatg gtcaccgcca acaacgcttc acgctgttcg cccagttcgc
    26281 agcgttccga gacgtagcgc aggccgtgga agttcacgtt ggcaccggaa agaatatgcg
    26341 ccagccgttc gccgcgaatg ttgtgctggg cgatatattt tttcattccc gccagcgcca
    26401 gcgcgccaga gggttccgcc accgcgcgca catcttcgaa taaatccttc atcgccgcac
    26461 agatcgcatc actatcgacg gtgatgatgt cgtcgagata ctcctggcat aaacggaagg
    26521 tttcgtcacc gatgcgtttt accgctacgc cttcagcaaa tagccctacg cgcggcagat
    26581 caaccggatg acccgcatcc agcgctgctt tcaggcaggc ggagtcttcc gcttctacgg
    26641 cgatcacttt gatttgtggc atcagttgtt tgatcagcac cgcaacgccc gccgccagac
    26701 cgccgccgcc gactggcaca aatacgcggt cgagatgggc gtcctgctgg agcagttcca
    26761 gcgccagcgt gccttgcccg gcaatcacca tcggatggtc gaacggcggc acccaggtga
    26821 acccctgctg ctgtgacagt tcgatcgctt tagctttcgc ttcgtcaaag ttcgcgccat
    26881 gaagcagcac ttcgccgccg aatccacgta ccgcatcgac tttgatgtcg gcggtggcgg
    26941 ttggcataac gatcagggcc ttcacgccta accgcgcaga agaaaacgcg acgccctgcg
    27001 cgtggttacc cgcagaagca gtgatcacgc cgtgcgcttt ttgttcttcc gtcaggcccg
    27061 ccatcatggc gtatgcgccg cgcagcttaa agctgtgcac tggctggcga tcttcgcgct
    27121 tcaccagaat gacgttatca agacgcgacg acagtttttc cattttttgt agcggcgtaa
    27181 cctgcgccgc ctcgtaaacc ggcgcgcgca gcactgctct taaatattcg gcaccttccg
    27241 gagcaccgga caggggttgc gagtcagcca ttattaaccc cccagtttcg atttatcgcg
    27301 caccgcacct ttgtcggcgc tggttgccag gctggcgtag gcgcgcaggg caaaggaaac
    27361 ctgacgttca cggtttttcg gcgtccaggc tttgtcacct cgagcgtcct gcgcttcacg
    27421 acgcgccgcc agttcggcat cgcttacctg taactgaatg ccacggttcg ggatgtctat
    27481 agcgatcagg tcaccatctt caatcaggcc aatgctgccg ccgcttgccg cttccggtga
    27541 gacgtggccg atggaaagac cagaggtgcc gccagagaaa cgaccgtcgg tgatcagcgc
    27601 acaggcttta ccgagaccca ttgatttcag gaagctggtt gggtagagca tttcctgcat
    27661 gccagggccg cctttcgggc cttcatagcg gatcaccacc acatcgcccg cgacaacttt
    27721 gccaccgagg atcgcttcta ccgcgtcgtc ctggctttcg tacactttcg ccgggccggt
    27781 gaatttgagg atgctgtcat cgacgcccgc ggttttaacg atgcagccgt tttctgcgaa
    27841 attaccgtag agcaccgcca ggccgccgtc tttgctgtag gcgtgttcca gcgagcggat
    27901 acagccattg gcgcgatcgt catcgagaga atcccaacgg caatcctgcg agaatgcctg
    27961 tgtggtacga atgcctgcag gacctgcgcg gaacatattt tttaccgcgt catcctgggt
    28021 cagcataacg tcgtattgtt ccagcgtttg cggcaacgtc aggccaagta cgtttttcac
    28081 atcacggttc agtaaccccg cgcgatccag ttcgccgaga ataccgataa caccaccagc
    28141 acggtgcaca tcttccatat ggtatttctg ggtgctcggc gcaactttac acagctgtgg
    28201 aaccttgcgg gaaagcttat cgatatcact catggtgaag tcgatttccg cttcctgcgc
    28261 cgccgccagc aggtgaagta cggtgttagt cgatccaccc atcgcgatat ccagcgtcat
    28321 ggcgttttca aacgccgctt tgctagcaat attgcgcggc agtgcacttt cgtcgttttg
    28381 ctcgtagtaa cgtttggtca attcaacaat gcgtttaccg gcattgagga acaactgctt
    28441 acggtcggcg tgggttgcca gcagcgagcc gttgcccggc tgcgacagac ccagcgcttc
    28501 ggtcaggcag ttcattgagt tagcggtaaa catcccggag caggagccgc aggtcggaca
    28561 cgcggaacgt tcaacctgat cgctctggga gtcagaaact ttcgggtctg cgccctggat
    28621 catcgcatca accagatcga gtttgattat ctgatcggac agtttggttt tcccggcctc
    28681 catcgggccg ccggaaacaa agatcaccgg aatattcagg cgcagggaag ccatcagcat
    28741 ccccggggtg attttgtcgc agttagagat gcagaccatg gcgtcggcgc agtgggcgtt
    28801 gaccatatac tcaacggaat cagcgatcag ttcgcgagat ggcagtgaat aaagcatccc
    28861 cccgtggccc atggcaatcc catcatccac cgcaatggtg ttgaactctt tggcaacgcc
    28921 gccagccgct tcaatttgtt cggcgaccag tttaccgaga tcgcgcagat ggacgtgacc
    28981 cggtacaaat tgggtgaacg agttcacaac cgcgataatc ggcttgccaa aatcggcgtc
    29041 ggtcattccg gtggcgcgcc acagcgcacg agcacccgcc atattacgac catgagtggt
    29101 ggtggcggaa cggtacttag gcatacttta tttactccca gtgtctgtct cgtaaatggg
    29161 acggtgcgtg ccgtcccatt ttttgtattt attgattaac ttgatctaac cagccccatt
    29221 tatcttcggt ttcgccagtg aagaggccga agaaggcttg ctgaatgcgt ttggtaaccg
    29281 ggccacaacg gccttcgcca acctgaatac catctacact gcgcactggc gtgatttctg
    29341 ccgcagtacc ggacataaac acttcatccg ccaggtacag ggattcgcgc gacagaacct
    29401 gctcacgcac ttcaattccc agctctttcg ccagtttgat gatggcatca cgggtaatac
    29461 ccggcagcgc ggaggaggtg aacggtgggg tgaacagcac accatctttc acttcaaaca
    29521 ggttttcgcc tgcgccttca gagatataac cgttcacatc cagcgcgata ccttcctgat
    29581 aaccgtggcg gcgcgcttcg ctacccacca gcagggaaga gagatagtta ccaccggctt
    29641 ttgccgcggt tgggatggtg tttggtgctg cgcggttcca ggaggaaacc atcgcatcga
    29701 tcccctgctc cagcgcttct gcgcccagat acgctcccca cgggaaagcg gcgataatca
    29761 catcggttga gtatcccgct ggcgggttta cacccatgcc aacatcacca acgaagatca
    29821 gcggacggat ataggcgctg gtgagattgt ttttgcggat cacgtcacga caagcttcca
    29881 tcagctcatc aatgctctgc gaaaccggga agcgatagat tttggcggag tcatgcagac
    29941 gctgcatatg ctcacgatgg cggaatacaa ccggtccttt gtgcgagtcg tagcaacgga
    30001 tgccttcaaa aaccgaagtg ccatagtgca gcgcgtgcga catcacatgc accttcgcgt
    30061 cttcccagcg aaccatctcc ccattgaacc aaatgtaatc agctttcttc gtggtcattt
    30121 ttatattcct tttgcgctca ggcgcggatt tgttgtgatg tggttgtgct ctggcagatg
    30181 gcaacgtgtg cgacgtccac cagtttattt aactgactaa acagtaagtc gaccgaccgt
    30241 gggctggcaa cggtcaattc gatatttata ttttgtgcat cgctggcggc agccatattc
    30301 attgagcaga cgtggaaacc acgatgacgc accacgcgta aaacacgttc taaggtttcc
    30361 ggattgaagc gagccgatac attgacctga tgttgcatca tgataatttc tccaacattt
    30421 ctgaattact ggcgccaggc ggcaccagcg gccagacgtt ctcaagttcg tcgattgaga
    30481 catgaagcag gtatggccca tcactgttca gcatggtgtc gagtgccgct tcaacctggt
    30541 ctttacgggt gatgtgttgg ccagggatgc cgaaggcgct ggctaacatg aggaaatcgg
    30601 ggttatcagt aagggtggtt tcgctgtatc gttcctgaaa aaacagttgc tgccattgtc
    30661 gaaccatccc taaccgttgg ttatcgagta agacgatttt caacggtaac tgcttgcgtt
    30721 ttacggtgcc cagctcttgc acattcatca tgaaagagcc gtcaccggag atacagacga
    30781 cagtatcgtt cggtcgtgcg acttgtgcgc caaccgccgc tggtaaaccg aaacccatgg
    30841 tgcctaagcc gctggaggta atgaaatttt ccgggcgagt gtgtgcgatg tgctgcgcgg
    30901 cccacatctg gtgctgcccc acatctgtgg tcacgacgca atccgcaggt ttacgatccg
    30961 acagttgttt taacaacaac ggcgcgtaga tagcgtcacc gggatggtcg taacgccagg
    31021 tatgttcatc acgcagctgc gcgcagtatt gctgccagtc attgatattt aacggctgct
    31081 gtaatgctgg taacagagca tttaaatcac cttgtaatgc cacatgtgcc tgacgcagct
    31141 tgttcatttc tgccgggtcg atatccatat ggataacact ggcgtgtggt gcgaaggtgt
    31201 tcagtttgcc ggtcacccgg tcatcaaaac gtgcacccac ggcgatcagc aagtcgcact
    31261 cctgcaccgc gaagttcgcc gctttggtgc catgcattcc cagcatgccc aggtagtacg
    31321 gataatctgc ttcaactgcg cccagccctt tcagcgtgca ggtggcaggc atttttgtgg
    31381 tagcgagaaa ttctcgtaaa gcaggaactg cctgcgccat acccacgcca ccaccaacgt
    31441 acagcatcgg tttttgcgct tttgccagca tctggcgcgc ttgctcaact tcggcatgtg
    31501 ggaaagtcac ttcgttttca acggtggtga accacggttc caggtcaccg ctggctagct
    31561 ggatatcttt tgggatatcg accagaaccg gaccaggacg acctgagctg gcaacgtcga
    31621 atgcttcagc cataatgcgc ggcaactctt ccagcgactg caccagaaag ctgtgcttgg
    31681 tacaggctaa cgacaatccc aggacatcca cttcctgaaa tgcgtccgtg ccgataaacg
    31741 gtgcggacac ttgaccggtg atggcaacaa cagggataga atctaacagt gcgtccgcaa
    31801 gcccggttat caggttggtt gcgcccggac cagacgtggc gatacatacg ccagttttgc
    31861 cggtagcacg ggcataaccg atagccgcca ttgccgcacc ctgctcatgt cggcacagca
    31921 agtgctccac gccgccgtca tacaatgcat cgtaaaccgg cataattgcg ccacccggat
    31981 aaccgaaaac ggtgttcaca ccctgtgccc gcaacgcatg taccacccac tgtgcgccat
    32041 tcatagttag ttccccgtcc tgaatcttga gaaacagaat tttgtgctgt tattcattgt
    32101 ctgctcctcg gttatatttt taaggtcaaa aaaaaccccc ggacctttcg gtgcgggggt
    32161 cttagttcgt taaggcttga tctctaagcc tttcctcgtc caagtgcagc cccgcacggt
    32221 gggataataa tcaccaccac gctaatcatg accaggctaa tcactcgtag aagggctgtc
    32281 attttgtctt ttcttgcatc ttgttcgaag gaatgcctaa agagttacca tagattttgc
    32341 gaatagcaca agata
//
LOCUS       DAZKHF020000021        31735 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2201S31241
            SAMN43267800-rid25106413.denovo.021, whole genome shotgun sequence.
ACCESSION   DAZKHF020000021 DAZKHF020000000
VERSION     DAZKHF020000021.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267800
            Sequence Read Archive: SRR30307263
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 31735)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 31735)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 31735)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350581.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350581.2
            Genome Coverage       :: 151x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:21:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,652
            CDSs (total)                      :: 5,557
            Genes (coding)                    :: 5,320
            CDSs (with protein)               :: 5,320
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 237
            CDSs (without protein)            :: 237
            Pseudo Genes (ambiguous residues) :: 0 of 237
            Pseudo Genes (frameshifted)       :: 102 of 237
            Pseudo Genes (incomplete)         :: 130 of 237
            Pseudo Genes (internal stop)      :: 52 of 237
            Pseudo Genes (multiple problems)  :: 41 of 237
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..31735
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267800-rid25106413.denovo.021"
                     /serotype="O 26:H11"
                     /isolate="2201S31241"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-01"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            complement(172..1059)
                     /gene="rarD"
                     /locus_tag="ACHOSC_RS04240"
                     /old_locus_tag="pgaptmp_000848"
     CDS             complement(172..1059)
                     /gene="rarD"
                     /locus_tag="ACHOSC_RS04240"
                     /old_locus_tag="pgaptmp_000848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000380001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter RarD"
                     /protein_id="HGU4546836.1"
                     /translation="MDAKQTRQGVLLALAAYFIWGIAPAYFKLIYYVPADEILTHRVI
                     WSFFFMVVLMSICRQWSYLKTLIQTPQKIFMLAVSAVLIGGNWLLFIWAVNNHHMLEA
                     SLGYFINPLVNIVLGMIFLGERFRRMQWLAVILAICGVLVQLWTFGSLPIIALGLAFS
                     FAFYGLVRKKIAVEAQTGMLIETMWLLPVAAIYLFAIADSSTSHMGQNPMSLNLLLIA
                     AGIVTTVPLLCFTAAATRLRLSTLGFFQYIGPTLMFLLAVTFYGEKPGADKMVTFAFI
                     WVALAIFVMDAIYTQRRKS"
     gene            complement(1111..1578)
                     /gene="yigI"
                     /locus_tag="ACHOSC_RS04245"
                     /old_locus_tag="pgaptmp_000849"
     CDS             complement(1111..1578)
                     /gene="yigI"
                     /locus_tag="ACHOSC_RS04245"
                     /old_locus_tag="pgaptmp_000849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312777.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioesterase YigI"
                     /protein_id="HGU4546837.1"
                     /translation="MSAVLTAEQALKLVGEMFVYHMPFNRALGMELERYEKEFAQLAF
                     KNQPMMVGNWAQSILHGGVIASALDVAAGLVCVGSTLTRHETISEDELRQRLSRMGTI
                     DLRVDYLRPGRGERFTATSSLLRAGNKVAVARVELHNEEQLYIASATATYMVG"
     gene            1743..2612
                     /gene="pldA"
                     /locus_tag="ACHOSC_RS04250"
                     /old_locus_tag="pgaptmp_000850"
     CDS             1743..2612
                     /gene="pldA"
                     /locus_tag="ACHOSC_RS04250"
                     /old_locus_tag="pgaptmp_000850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004620 - phospholipase activity [Evidence
                     IEA];
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipase A"
                     /protein_id="HGU4546838.1"
                     /translation="MRTLQGWLLPVFMLPMAVYAQEATVKEVHDAPAVRGSIIANMLQ
                     EHDNPFTLYPYDTNYLIYTQTSDLNKEAIASYDWAENARKDEVKFQLSLAFPLWRGIL
                     GPNSVLGASYTQKSWWQLSNSEESSPFRETNYEPQLFLGFATDYRFAGWTLRDVEMGY
                     NHDSNGRSDPTSRSWNRLYTRLMAENGNWLVEVKPWYVVGNTDDNPDITKYMGYYQLK
                     IGYHLGDAVLSAKGQYNWNTGYGGAELGLSYPITKHVRLYTQVYSGYGESLIDYNFNQ
                     TRVGVGVMLNDLF"
     gene            2745..4574
                     /gene="recQ"
                     /locus_tag="ACHOSC_RS04255"
                     /old_locus_tag="pgaptmp_000851"
     CDS             2745..4574
                     /gene="recQ"
                     /locus_tag="ACHOSC_RS04255"
                     /old_locus_tag="pgaptmp_000851"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026263.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecQ"
                     /protein_id="HGU4546839.1"
                     /translation="MAQAEVLNLESGAKQVLQETFGYQQFRPGQEEIIDTVLSGRDCL
                     VVMPTGGGKSLCYQIPALLLNGLTVVVSPLISLMKDQVDQLQANGVAAACLNSTQTRE
                     QQLEVMTGCRTGQIRLLYIAPERLMLDNFLEHLAHWNPVLLAVDEAHCISQWGHDFRP
                     EYAALGQLRQRFPTLPFMALTATADDTTRQDIVRLLGLNDPLIQISSFDRPNIRYMLM
                     EKFKPLDQLMRYVQEQRGKSGIIYCNSRAKVEDTAARLQSKGISAAAYHAGLENNVRA
                     DVQEKFQRDDLQIVVATVAFGMGINKPNVRFVVHFDIPRNIESYYQETGRAGRDGLPA
                     EAMLFYDPADMAWLRRCLEEKPQGQLQDIERHKLNAMGAFAEAQTCRRLVLLNYFGEG
                     RQEPCGNCDICLDPPKQYDGSTDAQIALSTIGRVNQRFGMGYVVEVIRGANNQRIRDY
                     GHDKLKVYGMGRDKSHEHWVSVIRQLIHLGLVTQNIAQHSALQLTEAARPVLRGESSL
                     QLAVPRIVALKPKAMQKSFGGNYDRKLFAKLRKLRKSIADESNVPPYVVFNDATLIEM
                     AEQMPITASEMLSVNGVGMRKLERFGKPFMALIRAHVDGDDEE"
     gene            4638..5258
                     /gene="rhtC"
                     /locus_tag="ACHOSC_RS04260"
                     /old_locus_tag="pgaptmp_000852"
     CDS             4638..5258
                     /gene="rhtC"
                     /locus_tag="ACHOSC_RS04260"
                     /old_locus_tag="pgaptmp_000852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine export protein RhtC"
                     /protein_id="HGU4546840.1"
                     /translation="MLMLFLTVAMVHIVALMSPGPDFFFVSQTAVSRSRKEAMMGVLG
                     ITCGVMVWAGIALLGLHLIIEKMAWLHTLIMVGGGLYLCWMGYQMLRGALKKEAVSAP
                     APQVELAKSGRSFLKGLLTNLANPKAIIYFGSVFSLFVGDNVGTTARWGIFALIIVET
                     LAWFTVVASLFALPQMRRGYQRLAKWIDGFAGALFAGFGIHLIISR"
     gene            complement(5320..5940)
                     /gene="rhtB"
                     /locus_tag="ACHOSC_RS04265"
                     /old_locus_tag="pgaptmp_000853"
     CDS             complement(5320..5940)
                     /gene="rhtB"
                     /locus_tag="ACHOSC_RS04265"
                     /old_locus_tag="pgaptmp_000853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine/homoserine lactone efflux protein"
                     /protein_id="HGU4546841.1"
                     /translation="MTLEWWFAYLLTSIILSLSPGSGAINTMTTSLNHGYRGAVASIA
                     GLQTGLAIHIVLVGVGLGTLFSRSVIAFEVLKWAGAAYLIWLGIQQWRAAGAIDLKSL
                     ASTQSRRHLFQRAVFVNLTNPKSIVFLAALFPQFIMPQQPQLMQYIVLGVTTIVVDII
                     VMIGYATLAQRIALWIKGPKQMKALNKIFGSLFMLVGALLASARHA"
     gene            6051..7073
                     /gene="pldB"
                     /locus_tag="ACHOSC_RS04270"
                     /old_locus_tag="pgaptmp_000854"
     CDS             6051..7073
                     /gene="pldB"
                     /locus_tag="ACHOSC_RS04270"
                     /old_locus_tag="pgaptmp_000854"
                     /EC_number="3.1.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipase L2"
                     /protein_id="HGU4546842.1"
                     /translation="MFQQQKDWETRENAFAAFTMGPLTDFWRQRDEAEFTGVDDIPVR
                     FVRFRAQHHDRVVVICPGRIESYVKYAELAYDLFHLGFDVLIIDHRGQGRSGRLLADP
                     HLGHVNRFNDYVDDLAAFWQQEVQPGPWRKRYILAHSMGGAISTLFLQRHPGVCDAIA
                     LTAPMFGIVIRMPSFMARQILNWAEAHPRFRDGYAIGTGRWRALPFAINVLTHSRQRY
                     RRNLRFYADDPTIRVGGPTYHWVRESILAGEQVLAGAGDDATPTLLLQAEEERVVDNR
                     MHDRFCELRTAAGHPVEGGRPLVIKGAYHEILFEKDAMRSVALHAIVDFFNRHNSPSG
                     NRSTEV"
     gene            7081..7881
                     /gene="yigL"
                     /locus_tag="ACHOSC_RS04275"
                     /old_locus_tag="pgaptmp_000855"
     CDS             7081..7881
                     /gene="yigL"
                     /locus_tag="ACHOSC_RS04275"
                     /old_locus_tag="pgaptmp_000855"
                     /EC_number="3.1.3.23"
                     /EC_number="3.1.3.74"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar/pyridoxal phosphate phosphatase YigL"
                     /protein_id="HGU4546843.1"
                     /translation="MYQVVASDLDGTLLSPDHTLSPYAKETLKLLTARGINFVFATGR
                     HHVDVGQIRDNLEIKSYMITSNGARVHDLDGNLIFAHNLDRDIASDLFGVVNDNPDII
                     TNVYRDDEWFMNRHRPEEMRFFKEAVFKYALYEPGLLEPEGVSKVFFTCDSHEQLLPL
                     EQAINARWGDRVNVSFSTLTCLEVMAGGVSKGHALEAVAKKLGYSLKDCIAFGDGMND
                     AEMLSMAGKGCIMGSAHQRLKDLHPELEVIGTNADDAVPHYLRKLYLS"
     gene            7957..8856
                     /gene="bioP"
                     /locus_tag="ACHOSC_RS04280"
                     /old_locus_tag="pgaptmp_000856"
     CDS             7957..8856
                     /gene="bioP"
                     /locus_tag="ACHOSC_RS04280"
                     /old_locus_tag="pgaptmp_000856"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin transporter"
                     /protein_id="HGU4546844.1"
                     /translation="MALLIITTILWAFSFSFYGEYLAGHVDSYFAVLVRVGLAALVFL
                     PFLRTRGNSLKTVGLYMLVGAMQLGVMYMLSFRAYLYLTVSELLLFTVLTPLYITLIY
                     DIMSKRRLRWGYAFSALLAVIGAGIIRYDQVTDHFWTGLLLVQLSNITFAIGMVGYKR
                     LMETRPMPQHNAFAWFYLGAFLVAVIAWFLLGNAQKMPQTTLQWGILVFLGVVASGIG
                     YFMWNYGATQVDAGTLGIMNNMHVPAGLLVNLAIWHQQPHWPTFITGALVILASLWVH
                     RKWVAPRSSQTADDRRRDCALSE"
     gene            complement(8744..9697)
                     /gene="metR"
                     /locus_tag="ACHOSC_RS04285"
                     /old_locus_tag="pgaptmp_000857"
     CDS             complement(8744..9697)
                     /gene="metR"
                     /locus_tag="ACHOSC_RS04285"
                     /old_locus_tag="pgaptmp_000857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator MetR"
                     /protein_id="HGU4546845.1"
                     /translation="MIEVKHLKTLQALRNCGSLAAAAATLHQTQSALSHQFSDLEQRL
                     GFRLFVRKSQPLRFTPQGEILLQLANQVLPQISQALQACNEPQQTRLRIAIECHSCIQ
                     WLTPALENFHKNWPQVEMDFKSGVTFDPQPALQQGELDLVMTSDILPRSGLHYSPMFD
                     YEVRLVLAPDHPLAAKTRITPEDLASETLLIYPVQRSRLDVWRHFLQPAGVSPSLKSV
                     DNTLLLIQMVAARMGIAALPHWVVESFERQGLVVTKTLGEGLWSRLYAAVRDGEQRQP
                     ITEAFIRSARNHACDHLPFVKSAERPTYDAPTVRPGSPARL"
     gene            9815..12076
                     /gene="metE"
                     /locus_tag="ACHOSC_RS04290"
                     /old_locus_tag="pgaptmp_000858"
     CDS             9815..12076
                     /gene="metE"
                     /locus_tag="ACHOSC_RS04290"
                     /old_locus_tag="pgaptmp_000858"
                     /EC_number="2.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003871 -
                     5-methyltetrahydropteroyltriglutamate-homocysteine
                     S-methyltransferase activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0008652 - amino acid biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009086 - methionine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-methyltetrahydropteroyltriglutamate--
                     homocysteine S-methyltransferase"
                     /protein_id="HGU4546846.1"
                     /translation="MTILNHTLGFPRVGLRRELKKAQESYWAGNSTREELLTVGRELR
                     ARHWDQQKQAGIDLLPVGDFAWYDHVLTTSLLLGNVPPRHQNKDGSVDIDTLFRIGRG
                     RAPTGEPAAAAEMTKWFNTNYHYMVPEFVKGQQFKLTWTQLLEEVDEALALGHNVKPV
                     LLGPVTYLWLGKVKGEQFDRLSLLNDILPVYQQVLAELAKRGIEWVQIDEPALVLELP
                     QAWLDAYKPAYDALQGQVKLLLTTYFEGVTPNLDTITALPVQGLHVDLVHGKDDVAEL
                     HKRLPSDWLLSAGLINGRNVWRADLTEKYAQIKDIVGKRDLWVASSCSLLHSPIDLSV
                     ETRLDAEVKSWFAFALQKCHELALLRDALNSGDTAALAEWSAPIQARRHSTRVHNPAV
                     EKRLAAITAQDSQRANVYEVRAEAQRARFKLPAWPTTTIGSFPQTTEIRTLRLDFKKG
                     NLDANNYRTGIAEHIRQAIVEQERLGLDVLVHGEAERNDMVEYFGEHLDGFVFTQNGW
                     VQSYGSRCVKPPIVIGDVSRPAPITVEWAKYAQSLTDKPVKGMLTGPVTILCWSFPRE
                     DVSRETIAKQIALALRDEVADLEAAGIGIIQIDEPALREGLPLRRSDWDAYLQWGVEA
                     FRINAAVAKDDTQIHTHMCYCEFNDIMDSIAALDADVITIETSRSDMELLESFEEFDY
                     PNEIGPGVYDIHSPNVPSVEWIEALLKKAAKRIPAERLWVNPDCGLKTRGWPETRAAL
                     ANMVQAAQNLRLG"
     gene            complement(12115..12930)
                     /gene="ysgA"
                     /locus_tag="ACHOSC_RS04295"
                     /old_locus_tag="pgaptmp_000859"
     CDS             complement(12115..12930)
                     /gene="ysgA"
                     /locus_tag="ACHOSC_RS04295"
                     /old_locus_tag="pgaptmp_000859"
                     /EC_number="3.-.-.-"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dienelactone hydrolase family protein"
                     /protein_id="HGU4546847.1"
                     /translation="MATTQQSGFAPAASPLASTIVQTPDDAIVAGFTSIPSQGDNMPA
                     YHARPKQSDGPLPVVIVVQEIFGVHEHIRDICRRLALEGYLAIAPELYFREGDPNDFA
                     DIPTLLSGLVAKVPDSQVLADLDHVASWASRNGGDVHRLMITGFCWGGRITWLYAAHN
                     PQLKAAVAWYGKLTGDKSLNSPKQPVDIATDLNAPVLGLYGGQDNSIPQESVETMRQA
                     LRAANAKAEIIVYPDAGHAFNADYRPSYHAESAKDGWQRMLEWFKQYGGKKSL"
     gene            13192..13953
                     /gene="udp"
                     /locus_tag="ACHOSC_RS04300"
                     /old_locus_tag="pgaptmp_000860"
     CDS             13192..13953
                     /gene="udp"
                     /locus_tag="ACHOSC_RS04300"
                     /old_locus_tag="pgaptmp_000860"
                     /EC_number="2.4.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016763 - pentosyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uridine phosphorylase"
                     /protein_id="HGU4546848.1"
                     /translation="MSKSDVFHLGLTKNDLQGATLAIVPGDPDRVEKIAALMDKPVKL
                     ASHREFTTWRAELDGKPVIVCSTGIGGPSTSIAVEELAQLGIRTFLRIGTTGAIQPHI
                     NVGDVLVTTASVRLDGASLHFAPLEFPAVADFECTTALVEAAKSIGATTHVGVTASSD
                     TFYPGQERYDTYSGRVVRHFKGSMEEWQAMGVMNYEMESATLLTMCASQGLRAGMVAG
                     VIVNRTQQEIPNAETMKQTESHAVKIVVEAARRLL"
     gene            14094..15521
                     /gene="rmuC"
                     /locus_tag="ACHOSC_RS04305"
                     /old_locus_tag="pgaptmp_000861"
     CDS             14094..15521
                     /gene="rmuC"
                     /locus_tag="ACHOSC_RS04305"
                     /old_locus_tag="pgaptmp_000861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA recombination protein RmuC"
                     /protein_id="HGU4546849.1"
                     /translation="MDFSIMVYAVIALVGVAIGWLFASYQHAQQKAEQLAEREEMVAE
                     LSAAKQQITQSEHWRAECELLNNEVRSLQSINTSLEADLREVTTRMEAAQQHADDKIR
                     QMINSEQRLSEQFENLANRIFEHSNRRVDEQNRQSLNSLLSPLREQLDGFRRQVQDSF
                     GKEAQERHTLTHEIRNLQQLNAQMAQEAINLTRALKGDNKTQGNWGEVVLTRVLEASG
                     LREGYEYETQVSIENDARSRMQPDVIVRLPQGKDVVIDAKMTLVAYERYFNAEDDYTR
                     ESALQEHIASVRNHIRLLGRKDYQQLPGLRTLDYVLMFIPVEPAFSLALDRQPELITE
                     ALKNNIMLVSPTTLLVALRTIANLWRYEHQSRNAQQIADRASKLYDKMRLFIDDMSAI
                     GQSLDKAQDNYRQAMKKLSSGRGNVLAQAEAFRGLGVEIKREINPDLAEQAVSQDEEY
                     RLRSVPEQPNDEAYQRDDEYNQQSR"
     gene            15616..16371
                     /gene="ubiE"
                     /locus_tag="ACHOSC_RS04310"
                     /old_locus_tag="pgaptmp_000862"
     CDS             15616..16371
                     /gene="ubiE"
                     /locus_tag="ACHOSC_RS04310"
                     /old_locus_tag="pgaptmp_000862"
                     /EC_number="2.1.1.163"
                     /EC_number="2.1.1.201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional demethylmenaquinone
                     methyltransferase/2-methoxy-6-polyprenyl-1,4-benzoquinol
                     methylase UbiE"
                     /protein_id="HGU4546850.1"
                     /translation="MVDKSQETTHFGFQTVAKEQKADMVAHVFHSVASKYDVMNDLMS
                     FGIHRLWKRFTIDCSGVRRGQTVLDLAGGTGDLTAKFSRLVGETGKVVLADINESMLK
                     MGREKLRNIGVIGNVEYVQANAEALPFPDNTFDCITISFGLRNVTDKDKALRSMYRVL
                     KPGGRLLVLEFSKPIIEPLSKAYDAYSFHVLPRIGSLVANDADSYRYLAESIRMHPDQ
                     DTLKAMMQDAGFESVDYYNLTAGVVALHRGYKF"
     gene            16385..16990
                     /gene="ubiJ"
                     /locus_tag="ACHOSC_RS04315"
                     /old_locus_tag="pgaptmp_000863"
     CDS             16385..16990
                     /gene="ubiJ"
                     /locus_tag="ACHOSC_RS04315"
                     /old_locus_tag="pgaptmp_000863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ubiquinone biosynthesis protein UbiJ"
                     /protein_id="HGU4546851.1"
                     /translation="MPFKPLVTAGIESLLNTFLYRSPALKTARSRLLGKVLRVEVKGF
                     STSLILVFSERQVDVLGEWAGDADCTVIAYASVLPKLRDRQQLTALIRSGELEVQGDI
                     QVVQNFVALADLAEFDPAELLAPYTGDIAAEGISKAMRGGAKFLHHGIKRQQRYVAEA
                     ITEEWRMAPGPLEVAWFAEETAAVERAVDALTKRLEKLEAK"
     gene            16987..18627
                     /gene="ubiB"
                     /locus_tag="ACHOSC_RS04320"
                     /old_locus_tag="pgaptmp_000864"
     CDS             16987..18627
                     /gene="ubiB"
                     /locus_tag="ACHOSC_RS04320"
                     /old_locus_tag="pgaptmp_000864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006744 - ubiquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ubiquinone biosynthesis regulatory protein
                     kinase UbiB"
                     /protein_id="HGU4546852.1"
                     /translation="MTPGEVRRLYFIIRTFLSYGLDELIPKMRITLPLRLWRYSLFWM
                     PNRHKDKLLGERLRLALQELGPVWIKFGQMLSTRRDLFPPHIADQLALLQDKVAPFDG
                     KLAKQQIEAAMGGLPVEAWFDDFEIKPLASASIAQVHTARLKSNGKEVVIKVIRPDIL
                     PVIKADLKLIYRLARWVPRLLPDGRRLRPTEVVREYEKTLIDELNLLRESANAIQLRR
                     NFEDSPMLYIPEVYPDYCSEGMMVMERIYGIPVSDVAALEKNGTNMKLLAERGVQVFF
                     TQVFRDSFFHADMHPGNIFVSYEHPENPKYIGIDCGIVGSLNKEDKRYLAENFIAFFN
                     RDYRKVAELHVDSGWVPPDTNVEEFEFAIRTVCEPIFEKPLAEISFGHVLLNLFNTAR
                     RFNMEVQPQLVLLQKTLLYVEGVGRQLYPQLDLWKTAKPFLESWIKDQVGIPALVRAF
                     KEKAPFWVEKMPELPELVYDSLRQGKYLQHSVDKIARELQSNHVRQGQSRYFLGIGAT
                     LVLSGTFLLVSRPEWGLMPGWLMAGGLIAWFVGWRKTR"
     gene            18706..18975
                     /gene="tatA"
                     /locus_tag="ACHOSC_RS04325"
                     /old_locus_tag="pgaptmp_000865"
     CDS             18706..18975
                     /gene="tatA"
                     /locus_tag="ACHOSC_RS04325"
                     /old_locus_tag="pgaptmp_000865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Sec-independent protein translocase subunit
                     TatA"
                     /protein_id="HGU4546853.1"
                     /translation="MGGISIWQLLIIAVIVVLLFGTKKLGSIGSDLGASIKGFKKAMS
                     DDEPKQDKTSQDADFTAKTIADKQADTNQEQAKTEDAKRHDKEQV"
     gene            18979..19494
                     /gene="tatB"
                     /locus_tag="ACHOSC_RS04330"
                     /old_locus_tag="pgaptmp_000866"
     CDS             18979..19494
                     /gene="tatB"
                     /locus_tag="ACHOSC_RS04330"
                     /old_locus_tag="pgaptmp_000866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008320 - protein transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Sec-independent protein translocase protein
                     TatB"
                     /protein_id="HGU4546854.1"
                     /translation="MFDIGFSELLLVFIIGLVVLGPQRLPVAVKTVAGWIRALRSLAT
                     TVQNELTQELKLQEFQDSLKKVEKASLTNLTPELKASMDELRQAAESMKRSYVANDPE
                     KASDEAHTIHNPVVKDNEAAHEGVTPAAAQTQASSPEQKPETTPEPVVKPAADAEPKT
                     AAPSPSSSDKP"
     gene            19497..20273
                     /gene="tatC"
                     /locus_tag="ACHOSC_RS04335"
                     /old_locus_tag="pgaptmp_000867"
     CDS             19497..20273
                     /gene="tatC"
                     /locus_tag="ACHOSC_RS04335"
                     /old_locus_tag="pgaptmp_000867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Sec-independent protein translocase subunit
                     TatC"
                     /protein_id="HGU4546855.1"
                     /translation="MSVEDTQPLITHLIELRKRLLNCIIAVIVIFLCLVYFANDIYHL
                     VSAPLIKQLPQGSTMIATDVASPFFTPIKLTFMVSLILSAPVILYQVWAFIAPALYKH
                     ERRLVVPLLVSSSLLFYIGMAFAYFVVFPLAFGFLANTAPEGVQVSTDIASYLSFVMA
                     LFMAFGVSFEVPVAIVLLCWMGITSPEDLRKKRPYVLVGAFVVGMLLTPPDVFSQTLL
                     AIPMYCLFEIGVFFSRFYVGKGRNREEENDAEAESEKTEE"
     gene            20315..21097
                     /gene="tatD"
                     /locus_tag="ACHOSC_RS04340"
                     /old_locus_tag="pgaptmp_000868"
     CDS             20315..21097
                     /gene="tatD"
                     /locus_tag="ACHOSC_RS04340"
                     /old_locus_tag="pgaptmp_000868"
                     /EC_number="3.1.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709645.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3'-5' ssDNA/RNA exonuclease TatD"
                     /protein_id="HGU4546856.1"
                     /translation="MFDIGVNLTSSQFAKDRDDVVARAFDAGVNGLLITGTNLRESQQ
                     AQKLARQYSSCWSTAGVHPHDSSQWQAATEEAIIELAAQPEVVAIGECGLDFNRNFST
                     PEEQERAFVAQLRIAAELNMPVFMHCRDAHERFMTLLEPWLDKLPGAVLHCFTGTREE
                     MQACVARGIYIGITGWVCDERRGLELRELLPLIPAEKLLIETDAPYLLPRDLTPKPSS
                     RRNEPAHLPHILQRIAHWRGEDAAWLAATTDANVKTLFGIAF"
     gene            complement(21094..21582)
                     /gene="rfaH"
                     /locus_tag="ACHOSC_RS04345"
                     /old_locus_tag="pgaptmp_000869"
     CDS             complement(21094..21582)
                     /gene="rfaH"
                     /locus_tag="ACHOSC_RS04345"
                     /old_locus_tag="pgaptmp_000869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription/translation regulatory transformer
                     protein RfaH"
                     /protein_id="HGU4546857.1"
                     /translation="MQSWYLLYCKRGQLQRAQEHLERQAVNCLAPMITLEKIVRGKRT
                     AVSEPLFPNYLFVEFDPEVIHTTTINATRGVSHFVRFGASPAIVPSAVIHQLSVYKPK
                     DIVDPATPYPGDKVIITEGAFEGFQAIFTEPDGEARSMLLLNLINKEIKHSVKNTEFR
                     KL"
     gene            21749..23242
                     /gene="ubiD"
                     /locus_tag="ACHOSC_RS04350"
                     /old_locus_tag="pgaptmp_000870"
     CDS             21749..23242
                     /gene="ubiD"
                     /locus_tag="ACHOSC_RS04350"
                     /old_locus_tag="pgaptmp_000870"
                     /EC_number="4.1.1.98"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016831 - carboxy-lyase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-3-polyprenylbenzoate decarboxylase"
                     /protein_id="HGU4546858.1"
                     /translation="MDAMKYNDLRDFLTLLEQQGELKRITLPVDPHLEITEIADRTLR
                     AGGPALLFENPKGYSMPVLCNLFGTPKRVAMGMGQEDVSALREVGKLLAFLKEPEPPK
                     GFRDLFDKLPQFKQVLNMPTKRLRGAPCQQKIVSGDDVDLNRIPIMTCWPEDAAPLIT
                     WGLTVTRGPHKERQNLGIYRQQLIGKNKLIMRWLSHRGGALDYQEWCAAHPGERFPVS
                     VALGADPATILGAVTPVPDTLSEYAFAGLLRGTKTEVVKCISNDLEVPASAEIVLEGY
                     IEQGETAPEGPYGDHTGYYNEVDSFPVFTVTHITQREDAIYHSTYTGRPPDEPAVLGV
                     ALNEVFVPILQKQFPEIVDFYLPPEGCSYRLAVVTIKKQYAGHAKRVMMGVWSFLRQF
                     MYTKFVIVCDDDVNARDWNDVIWAITTRMDPARDTVLVENTPIDYLDFASPVSGLGSK
                     MGLDATNKWPGETQREWGRPIKKDPDVVAHIDAIWDELAIFNNGKSA"
     gene            23288..23989
                     /gene="fre"
                     /locus_tag="ACHOSC_RS04355"
                     /old_locus_tag="pgaptmp_000871"
     CDS             23288..23989
                     /gene="fre"
                     /locus_tag="ACHOSC_RS04355"
                     /old_locus_tag="pgaptmp_000871"
                     /EC_number="1.5.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-flavin reductase"
                     /protein_id="HGU4546859.1"
                     /translation="MTTLSCKVTSVEAITDTVYRVRIVPDAAFSFRAGQYLMVVMDER
                     DKRPFSMASTPDEKGFIELHIGASEINLYAKAVMDRILKDHQIVVDIPHGEAWLRDDE
                     ERPMILIAGGTGFSYARSILLTALARNPNRDITIYWGGREEQHLYDLCELEALSLKHP
                     GLQVVPVVEQPEAGWRGRTGTVLTAVLQDHGTLAEHDIYIAGRFEMAKIARDLFCSER
                     NAREDRLFGDAFAFI"
     gene            complement(24181..25344)
                     /gene="fadA"
                     /locus_tag="ACHOSC_RS04360"
                     /old_locus_tag="pgaptmp_000872"
     CDS             complement(24181..25344)
                     /gene="fadA"
                     /locus_tag="ACHOSC_RS04360"
                     /old_locus_tag="pgaptmp_000872"
                     /EC_number="2.3.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003988 - acetyl-CoA C-acyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006631 - fatty acid metabolic process
                     [Evidence IEA];
                     GO_process: GO:0016042 - lipid catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acyltransferase FadA"
                     /protein_id="HGU4546860.1"
                     /translation="MEQVVIVDAIRTPMGRSKGGAFRNVRAEDLSAHLMRSLLARNPA
                     LEAAALDDIYWGCVQQTLEQGFNIARNAALLAEVPHSVPAVTVNRLCGSSMQALHDAA
                     RMIMTGDAQACLVGGVEHMGHVPMSHGVDFHPGLSRNVAKAAGMMGLTAEMLARMHGI
                     SREMQDAFAARSHARAWAATQSAAFKNEIIPTGGHDADGVLKQFNYDEVIRPETTVEA
                     LATLRPAFDPVNGTVTAGTSSALSDGAAAMLVMSESRAHELGLKPRARVRSMAVVGCD
                     PSIMGYGPVPASKLALKKAGLSASDIGVFEMNEAFAAQILPCIKDLGLIEQIDEKINL
                     NGGAIALGHPLGCSGARISTTLLNLMERKDVQFGLATMCIGLGQGIATVFERV"
     gene            complement(25354..27543)
                     /gene="fadB"
                     /locus_tag="ACHOSC_RS04365"
                     /old_locus_tag="pgaptmp_000873"
     CDS             complement(25354..27543)
                     /gene="fadB"
                     /locus_tag="ACHOSC_RS04365"
                     /old_locus_tag="pgaptmp_000873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0036125 - fatty acid beta-oxidation
                     multienzyme complex [Evidence IEA];
                     GO_function: GO:0003857 - 3-hydroxyacyl-CoA dehydrogenase
                     activity [Evidence IEA];
                     GO_function: GO:0004165 - delta(3)-delta(2)-enoyl-CoA
                     isomerase activity [Evidence IEA];
                     GO_function: GO:0004300 - enoyl-CoA hydratase activity
                     [Evidence IEA];
                     GO_function: GO:0008692 - 3-hydroxybutyryl-CoA epimerase
                     activity [Evidence IEA];
                     GO_process: GO:0009062 - fatty acid catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acid oxidation complex subunit alpha FadB"
                     /protein_id="HGU4546861.1"
                     /translation="MLYKGDTLYLDWLEDGIAELVFDAPGSVNKLDTATVASLGEAIG
                     VLEQQSDLKGLLLRSNKAAFIVGADITEFLSLFLVPEEQLSQWLHFANSVFNRLEDLP
                     VPTIAAVNGYALGGGCECVLATDYRLATPDLRIGLPETKLGIMPGFGGSVRMPRMLGA
                     DSALEIIAAGKDVGADQALKIGLVDGVVKAEKLVEGAKAVLRQAINGDLDWKAKRQPK
                     LEPLKLSKIEATMSFTIAKGMVAQTAGKHYPAPITAVKTIEAAARFGREEALNLENKS
                     FVPLAHTNEARALVGIFLNDQYVKGKAKKLTKDVETPKQAAVLGAGIMGGGIAYQSAW
                     KGVPVVMKDINDKSLTLGMTEAAKLLNKQLERGKIDGLKLAGVISTIHPTLDYAGFDR
                     VDIVVEAVVENPKVKKAVLAETEQKVRQDTVLASNTSTIPISELANALKRPENFCGMH
                     FFNPVHRMPLVEIIRGEKSSDETIAKVVAWASKMGKTPIVVNDCPGFFVNRVLFPYFA
                     GFSQLLRDGADFRKIDKVMEKQFGWPMGPAYLLDVVGIDTAHHAQAVMAAGFPQRMQK
                     DYRDAIDALFDANRFGQKNGLGFWRYKEDSKGKPKKEEDAAVEDLLAEVSQPKRDFSE
                     EEIIARMMIPMVNEVVRCLEEGIIATPAEADMALVYGLGFPPFHGGAFRWLDTLGSAK
                     YLDMAQQYQHLGPLYEVPEGLRNKARHNEPYYPPVEPARPVGDLKTA"
     gene            27733..29064
                     /gene="pepQ"
                     /locus_tag="ACHOSC_RS04370"
                     /old_locus_tag="pgaptmp_000874"
     CDS             27733..29064
                     /gene="pepQ"
                     /locus_tag="ACHOSC_RS04370"
                     /old_locus_tag="pgaptmp_000874"
                     /EC_number="3.4.13.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Xaa-Pro dipeptidase"
                     /protein_id="HGU4546862.1"
                     /translation="MESLASLYKNHIATLQERTRDALARFKLDALLIHSGELFNVFLD
                     DHPYPFKVNPQFKAWVPVTQVPNCWLLVDGVNKPKLWFYLPVDYWHNVEPLPTSFWTE
                     DVEVIALPKADGIGSLLPAARGNIGYIGPVPERALQLGIEASNINPKGVIDYLHYYRS
                     FKTEYELACMREAQKMAVNGHRAAEEAFRSGMSEFDINIAYLTATGHRDTDVPYSNIV
                     ALNEHAAVLHYTKLDHQAPEEMRSFLLDAGAEYNGYAADLTRTWSAKSDNDYAQLVKD
                     VNDEQLALIATMKAGVSYVDYHIQFHQRIAKLLRKHQIITDMSEEAMVENDLTGPFMP
                     HGIGHPLGLQVHDVAGFMQDDSGTHLAAPAKYPYLRCTRILQPGMVLTIEPGIYFIES
                     LLAPWREGQFSKHFNWQKIEALKPFGGIRIEDNVVIHENNVENMTRDLKLA"
     gene            29064..29678
                     /gene="yigZ"
                     /locus_tag="ACHOSC_RS04375"
                     /old_locus_tag="pgaptmp_000875"
     CDS             29064..29678
                     /gene="yigZ"
                     /locus_tag="ACHOSC_RS04375"
                     /old_locus_tag="pgaptmp_000875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418290.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IMPACT family protein"
                     /protein_id="HGU4546863.1"
                     /translation="MESWLIPAAPVTVVEEIKKSRFITMLAHTDGVEAAKAFVESVRA
                     EHPDARHHCVAWVAGAPDDSQQLGFSDDGEPAGTAGKPMLAQLMGSGVGEITAVVVRY
                     YGGILLGTGGLVKAYGGGVNQALRQLTTQRKTPLTEYTLQCEYHQLTGIEALLGQCDG
                     KIINSDYQAFVLLRVALPAAKVAEFSAKLADFSRGSLQLLAIEE"
     gene            29717..31168
                     /gene="trkH"
                     /locus_tag="ACHOSC_RS04380"
                     /old_locus_tag="pgaptmp_000876"
     CDS             29717..31168
                     /gene="trkH"
                     /locus_tag="ACHOSC_RS04380"
                     /old_locus_tag="pgaptmp_000876"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004146450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Trk system potassium transporter TrkH"
                     /protein_id="HGU4546864.1"
                     /translation="MHFRAITRIVGLLVILFSGTMIIPGLVALIYRDGAGRAFTQTFF
                     VALAIGSMLWWPNRKEKGELKSREGFLIVVLFWTVLGSVGALPFIFSESPNLTITDAF
                     FESFSGLTTTGATTLVGLDSLPHAILFYRQMLQWFGGMGIIVLAVAILPILGVGGMQL
                     YRAEMPGPLKDNKMRPRIAETAKTLWLIYVLLTVACALALWFAGMDAFDAIGHSFATI
                     AIGGFSTHDASIGYFDSPTINTIIAIFLLISGCNYGLHFSLLSGRSLKVYWRDPEFRM
                     FIGVQFTLVVICTLVLWFHNVYSSALMTINQAFFQVVSMATTAGFTTDSIARWPLFLP
                     VLLLCSAFIGGCAGSTGGGLKVIRILLLFKQGNRELKRLVHPNAVYSIKLGNRALPER
                     ILEAVWGFFSAYALVFIVSMLAIIATGVDDFSAFASVVATLNNLGPGLGVVADNFTSM
                     NPVAKWILIANMLFGRLEVFTLLVLFTPTFWRE"
     gene            31180..31725
                     /gene="hemG"
                     /locus_tag="ACHOSC_RS04385"
                     /old_locus_tag="pgaptmp_000877"
     CDS             31180..31725
                     /gene="hemG"
                     /locus_tag="ACHOSC_RS04385"
                     /old_locus_tag="pgaptmp_000877"
                     /EC_number="1.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="menaquinone-dependent protoporphyrinogen IX
                     dehydrogenase"
                     /protein_id="HGU4546865.1"
                     /translation="MKTLILFSTRDGQTREIASYLASELKELGIQTDVANVHRIEEPQ
                     WENYDRVVIGASIRYGHYHSAFQEFVKKHATRLNSMPSAFYSVNLVARKPEKRTPQTN
                     SYARKFLMNSQWRPDRCAVIAGALRYPRYRWYDRFMIKLIMKMSGGETDTRKEVVYTD
                     WEQVANFAREIAHLTDKPTLK"
BASE COUNT         7335 a         8095 c         8704 g         7601 t
ORIGIN      
        1 tttgtcaaat ccttggtaga tatacgatgt ctctaggatg gagtaagtac tggggagttt
       61 tgggggtatt tcctgtaaca aatattgttc tttttcttat cttaattttt ttcaaaaaaa
      121 gataaataag tcagtgggta ggctctttaa acggcctacc catgttaatt cttaactttt
      181 tctacgctgc gtataaatcg catccatcac aaaaattgcc agcgccaccc aaataaaggc
      241 gaaagtcacc atcttatcgg cacccggttt ttcaccataa aacgtcacag ccagcaggaa
      301 catcagcgtc gggccaatgt actggaaaaa gcctaacgtt gagagacgca agcgcgtggc
      361 agcggcggta aaacacaaca gcggtacggt agtgacaata ccggcggcga tcagcagtaa
      421 attcagcgac atcgggtttt gccccatatg gctggttgag ctgtcggcaa tagcaaacag
      481 gtaaattgcc gccacgggca gcagccacat ggtttcgatt aacatgccgg tttgcgcttc
      541 aacggcaatc ttcttgcgta ccagaccgta gaaggcaaaa ctaaatgcca gtcccagcgc
      601 gataataggt agcgaaccaa aagtccacag ctggactaac acgccacata tcgccagaat
      661 caccgccagc cattgcatcc ggcggaatcg ctcgccgagg aaaatcatcc ccagcacaat
      721 gttcaccagc gggttaataa agtaaccaag gctcgcttcc agcatatggt gattgttcac
      781 cgcccagata aacagtagcc agttgccacc aatcagcacg gcagagactg ccagcataaa
      841 aattttctgt ggcgtctgaa tcagcgtttt taaataggac cactggcggc aaatgctcat
      901 cagcaccacc ataaagaaaa acgaccagat cacgcgatgc gtcaggattt catcggcagg
      961 cacgtagtaa atcaacttga agtacgctgg cgctataccc caaataaaat aagcggcaag
     1021 agcgagtaat acgccctgcc gcgtttgttt tgcatccatc gggaatactc atttttaatt
     1081 ggtaacagca gtttacctgc ttttatgtct tcaacctacc atataggtgg cggtggcact
     1141 ggcaatataa agctgttctt cattgtgtaa ttcaacgcgg gcgacggcga ctttattgcc
     1201 tgcacgcaac aggctactgg tagcagtaaa acgctcgccc ctgcctgggc gcagataatc
     1261 aacgcgaaga tcaatggtcc ccatccgcga tagccgctgg cgtagttcat cttcactgat
     1321 ggtttcgtgg cgggttaagg tacttcccac gcacaccaga cctgcggcga catccagcgc
     1381 tgacgcaata accccgccgt gcaaaatgct ttgcgcccag ttacccacca tcattggctg
     1441 atttttaaag gccagctgtg cgaactcttt ttcgtaacgc tccagctcca tccctaacgc
     1501 gcggttaaat ggcatgtgat aaacaaacat ctcacccact aatttcaggg cttgttcagc
     1561 ggtcagtacg gcagacatat catccttaca cttcattggt taatgaaatg ttgattttat
     1621 gcttctttgt tgttggtttc tactttagga agggataact aacggctatg gagttaagta
     1681 tgtaaaatag cccgcagaaa aatattcacc ttatcaataa ttcgttacgg agaacacgac
     1741 cgatgcggac tctgcagggc tggttgttgc cggtgtttat gttgcctatg gcagtatatg
     1801 cacaagaggc aacggtgaaa gaggtgcatg acgcgccagc ggtgcgtggc agtattatcg
     1861 ccaatatgct gcaggagcat gacaatccgt tcacgctcta tccttatgac accaactacc
     1921 tcatttacac ccaaaccagc gatctgaata aagaagcgat tgccagttac gactgggcgg
     1981 aaaatgcgcg taaggatgaa gtaaagtttc agttgagcct ggcatttccg ctgtggcgtg
     2041 ggattttagg cccgaactcg gtgttgggtg cgtcttatac gcaaaaatcc tggtggcaac
     2101 tgtccaatag cgaagagtct tcaccgtttc gtgaaaccaa ctacgaaccg caattgttcc
     2161 tcggttttgc caccgattac cgttttgcag gttggacgct gcgcgatgtg gagatggggt
     2221 ataaccacga ctctaacggg cgttccgacc cgacctcccg cagctggaac cgcctttata
     2281 ctcgcctgat ggcagaaaac ggtaactggc tggtagaagt gaagccgtgg tatgtggtgg
     2341 gtaatactga cgataacccg gatatcacca aatatatggg ttactaccag cttaaaatcg
     2401 gctatcacct cggtgatgcg gtgctcagtg cgaaaggaca gtacaactgg aacaccggct
     2461 acggcggcgc ggagttaggc ttaagttacc cgatcaccaa acatgtgcgc ctttatactc
     2521 aggtttacag cggctatggc gaatcgctca tcgactataa cttcaaccag acccgtgtcg
     2581 gtgtgggggt tatgctaaac gatttgtttt gatgaacggt tgagtggttg gcaaatctgg
     2641 aacccagcat ccaggattac cctctcagag actaaaagca ttgcagtttc tcgcgcaggc
     2701 gctgaaaata gcgcctgttt ttatttcagg caatcggggt gaatgtggcg caggcggaag
     2761 tgttgaatct ggagtccgga gctaaacagg ttttacaaga aacctttggc taccaacagt
     2821 ttcgccccgg ccaggaagaa attatcgaca ctgtgctttc cggtcgcgat tgcctggtcg
     2881 tcatgcccac cggtggcgga aaatcccttt gctatcaaat tcctgcctta ttgctaaacg
     2941 gccttaccgt ggttgtttca ccgctgattt cgttgatgaa agatcaggtg gatcaactgc
     3001 aagccaacgg cgtggcggcg gcgtgcctta actcgacgca aacccgcgaa cagcaacttg
     3061 aagtgatgac aggctgccgc accgggcaaa ttcgtctgct ttatatcgcc ccggaacgcc
     3121 tgatgctgga taactttctt gagcatctgg cgcactggaa tccggtgtta ttagccgttg
     3181 atgaagcgca ctgtatctcc caatggggcc acgatttccg cccggaatat gccgcgctcg
     3241 gtcagttgcg ccagcggttc ccgacgctgc cgtttatggc gctgaccgcc acagccgacg
     3301 acaccacgcg ccaggatatc gtgcgcctgc tggggctgaa cgatccgctg attcaaatca
     3361 gcagttttga ccgtccgaat attcgctaca tgctgatgga gaagttcaaa ccgctcgatc
     3421 agttgatgcg ctacgtgcag gaacagcgcg gtaagtccgg cattatctac tgcaacagcc
     3481 gggcgaaagt agaagacacc gctgcgcgcc tgcaaagcaa gggtattagc gcggcggcct
     3541 atcatgccgg gctggaaaat aatgtccgcg ccgacgtgca ggagaaattc cagcgcgatg
     3601 acctgcaaat tgtggtggcg acggtggcgt tcggcatggg catcaataaa ccaaacgttc
     3661 gcttcgtggt ccactttgat attccgcgca atatcgaatc ctattatcag gaaaccggtc
     3721 gcgccgggcg tgatggcctg cccgcggaag cgatgctgtt ttacgatccg gccgatatgg
     3781 cgtggctgcg ccgttgtctg gaagagaagc cgcaggggca gttgcaggat atcgaacgtc
     3841 ataaactcaa tgcgatgggc gcttttgccg aagcgcaaac ttgccgtcgt ctggtactgc
     3901 tgaactattt tggcgaaggg cgtcaggagc cgtgcgggaa ctgcgatatc tgcctcgatc
     3961 cgccgaaaca gtacgacggt tcaaccgatg ctcagattgc cctttccacc attggtcgtg
     4021 tgaatcagcg ttttggtatg ggctatgtgg tggaagtgat tcgcggcgct aataaccagc
     4081 gtatccgcga ctatggtcat gacaaactaa aagtctacgg tatgggccgt gataaaagtc
     4141 atgaacattg ggtgagcgtg atccgccagc tgattcacct cggtctggtg acgcaaaata
     4201 ttgcccagca ttctgcccta caactgacag aggctgcgcg tccggtgctg cgcggcgaat
     4261 cctctttgca acttgccgtg ccgcgtatcg tggcgctcaa accgaaagcg atgcagaaat
     4321 cgttcggcgg caactatgat cgcaaactgt tcgccaaatt acgcaaactg cgtaaatcga
     4381 tagccgatga aagtaatgtc ccgccgtacg tggtgtttaa cgacgcaacc ttgattgaga
     4441 tggctgaaca gatgccgatc accgccagcg aaatgctcag cgttaacggc gttgggatgc
     4501 gcaagctgga acgctttggc aaaccgttta tggcgctgat tcgtgcgcat gttgatggcg
     4561 atgacgaaga gtagtcagca gcataaaaaa gtgccagtat gaagactccg taaacgtttc
     4621 ccccgcgagt caaatgtatg ttgatgttat ttctcaccgt cgccatggtg cacattgtgg
     4681 cgcttatgag ccccggtccc gatttctttt ttgtctctca gaccgctgtc agtcgttccc
     4741 gtaaagaagc gatgatgggc gtgctgggca ttacctgcgg cgtaatggtt tgggctggga
     4801 ttgcgctgct tggcctgcat ttgattatcg aaaaaatggc ctggctgcat acgctgatta
     4861 tggtgggcgg tggcctgtat ctctgctgga tgggttacca gatgctacgc ggtgcactga
     4921 aaaaagaggc ggtttctgca cctgcgccac aggtcgagct ggcgaaaagt gggcgcagtt
     4981 tcctgaaagg tttactgacc aatctcgcta atccgaaagc gattatctac tttggctcgg
     5041 tgttctcatt gtttgtcggt gataacgttg gcactaccgc gcgctggggc atttttgcgc
     5101 tgatcattgt cgaaacgctg gcgtggttta ccgtcgttgc cagcctgttt gccctgccgc
     5161 aaatgcgccg tggttatcaa cgtctggcga agtggattga tggttttgcc ggggcgttat
     5221 ttgccggatt tggcattcat ttgattattt cgcggtgatg ccagacgcgt cttcagagta
     5281 agtcggataa ggcgtttacg ccgcatccga cattattttt cacgcatgcc tcgccgatgc
     5341 taacagcgct cccaccagca taaacaacga gccgaaaatc ttattcagcg ccttcatctg
     5401 ctttggtcct ttaatccata gagcaatccg ttgagcaagg gtggcgtaac cgatcatcac
     5461 aataatatcg accacaatag tggtgacgcc gagcacgata tactgcatca gttgcggctg
     5521 ttgcggcatg atgaattgcg gaaatagcgc cgccagaaac acaatacttt tgggattggt
     5581 gagattcaca aaaactgcgc gctggaacaa atgtcgacgc gattgagtag aggccagcga
     5641 tttaaggtca attgcaccag cggcgcgcca ctgctggatt cccagccaaa tcaagtaagc
     5701 cgcgcctgcc cacttcaaca cttcaaacgc aatcactgag cgggaaaata gcgtccccaa
     5761 ccccacgcca accagcacaa tatgaatcgc cagtccggtc tgaagcccag caatagacgc
     5821 caccgcgccg cgataaccgt ggttgagcga ggtggtcata gtgttgattg caccagagcc
     5881 tggcgacagg cttaaaatga tcgatgtcag caggtaggca aaccaccatt ctaaggtcat
     5941 gatgaactcc cggtgtgtct atttttgtgc cacaatacgc tactgtcgca gcgttgtgtc
     6001 aggcacgcta aaaaaaacga ttttacgtgg tttaagaggc agattacccg atgtttcagc
     6061 agcaaaaaga ctgggaaaca agagaaaacg cgtttgctgc ttttaccatg ggaccgctga
     6121 ctgatttctg gcgtcagcgt gatgaagcag agtttactgg tgtggatgac attccggtgc
     6181 gctttgtccg ttttcgcgca cagcaccatg accgggtggt agtcatctgc ccggggcgta
     6241 ttgagagcta cgtaaaatat gcggaactgg cctatgacct gttccatttg gggtttgatg
     6301 tcttaatcat cgaccatcgc gggcagggac gttccggtcg cctgttagcc gatccgcatc
     6361 tcgggcatgt taatcgcttt aatgattatg ttgatgatct ggcggcattc tggcagcagg
     6421 aggttcagcc cggtccgtgg cgtaaacgct atatactggc acattcgatg ggcggtgcga
     6481 tctccacatt atttctgcaa cgccatccag gtgtatgtga cgccattgcg ctaactgcgc
     6541 caatgtttgg gatcgtgatt cgtatgccgt catttatggc acggcagatc ctcaactggg
     6601 ccgaagcgca tccacgtttc cgtgatggct atgcaatagg caccgggcgc tggcgcgcgt
     6661 tgccgtttgc tatcaacgta ctgacccaca gcagacagcg atatcgacgt aacttacgct
     6721 tctatgctga tgacccaacg attcgcgtcg gtgggccgac ctaccattgg gtacgcgaaa
     6781 gtattctggc tggcgaacag gtgttagccg gtgcgggtga tgacgccacg ccaacgcttc
     6841 tcttacaagc tgaagaggaa cgcgtggtgg ataaccgcat gcatgaccgt ttttgtgaac
     6901 tccgcaccgc cgcgggccat cctgtcgaag gaggacggcc gttggtaatt aaaggtgctt
     6961 accatgagat cctttttgaa aaggacgcaa tgcgctcagt cgcgctccac gccatcgttg
     7021 attttttcaa caggcataac tcacccagcg gaaaccgctc tacagaggtt taaatttctt
     7081 atgtaccagg ttgttgcgtc tgatttagat ggcacgttac tttctcccga ccatacgtta
     7141 tccccttacg ccaaagaaac tctgaagctg ctcaccgcgc gcggcatcaa ctttgtgttt
     7201 gcgaccggtc gtcaccacgt tgatgtgggg caaattcgcg ataatctgga gattaagtct
     7261 tacatgatta cctccaatgg tgcgcgcgtt cacgatctgg atggtaatct gatttttgct
     7321 cataacctgg atcgcgacat tgccagcgat ctgtttggcg tagtcaacga caatccggac
     7381 atcattacta acgtttatcg cgacgacgaa tggtttatga atcgccatcg cccggaagag
     7441 atgcgctttt ttaaagaagc ggtgttcaaa tatgcgctgt atgagcctgg attactggag
     7501 ccggaaggcg tcagcaaagt gtttttcacc tgcgattccc atgaacaact gctgccgctg
     7561 gagcaggcga ttaacgctcg ctggggcgat cgcgtcaacg tcagtttctc taccttaacc
     7621 tgtctggaag tgatggcggg cggcgtgtcg aaaggccatg cgctggaagc agtggcgaag
     7681 aaactgggct acagcctgaa ggattgtatt gcgtttggtg acgggatgaa cgacgccgaa
     7741 atgctgtcga tggcggggaa aggctgcatt atgggcagtg cgcaccagcg tctgaaagac
     7801 cttcatcccg agctggaagt gattggtact aatgccgacg acgcggtgcc gcattatctg
     7861 cgtaaactct atttatcgta atcgttcttt atttggtcag ttgtcaacct gatacttcgc
     7921 tacaatggat actcgttaat caaagagttt tccattgtgg cgctacttat catcaccacg
     7981 attctgtggg ccttctcctt tagcttttat ggcgagtacc ttgcggggca cgtcgatagc
     8041 tattttgcgg tgctggtgcg cgttggcctg gcggcactcg tttttctgcc gtttctgcgt
     8101 acccgtggca atagcctgaa aacggtcggc ctgtatatgt tggtgggcgc gatgcagctc
     8161 ggcgtgatgt atatgctgag tttccgcgcg tatctctacc tgacggtctc cgaactgctg
     8221 ctgttcaccg tactgacgcc gctctacatc acgctgattt atgacatcat gagtaagcgc
     8281 cgtctgcgct ggggctatgc ctttagcgcc ttgttggcgg tgattggtgc cgggattatt
     8341 cgctatgatc aggtcaccga ccatttctgg actggcttgc tgctggtgca actctccaat
     8401 atcacttttg ccatcggcat ggtgggttac aaacgcctga tggaaactcg cccgatgcca
     8461 cagcataacg cctttgcgtg gttctatctt ggcgcgtttc tggtggcggt aattgcatgg
     8521 ttcttgctgg gaaatgcgca gaaaatgccg caaaccacgt tgcaatgggg cattctggtg
     8581 tttcttggcg tggtggcttc cgggattggc tactttatgt ggaactacgg cgcgacgcag
     8641 gtggacgccg gaacgctggg cattatgaat aatatgcacg ttccggcagg gctgctggta
     8701 aacctggcta tctggcacca acagccgcac tggccaacgt tcattacagg cgcgctggtg
     8761 atcctggcct cactgtgggt gcatcgtaag tgggtcgctc cgcgctcttc acaaacggca
     8821 gatgatcgca ggcgtgattg cgcgctgagc gaataaatgc ttctgtaatc ggctgacgct
     8881 gctcgccatc gcgcacggcg gcgtacagtc ggctccacaa gccttcgccc agggttttgg
     8941 tcaccaccag accctggcgc tcaaaactct ctactaccca atgcggtagc gcggcaatac
     9001 ccatccgcgc ggcaaccatc tgaatcaaca ataaggtgtt atcgacgctt ttcagtgacg
     9061 ggctgacgcc tgccggctga agaaaatgcc gccagacatc cagtcgacta cgctgcaccg
     9121 gataaatcaa cagcgtctcg ctggcgagat cttccggtgt aattcgcgtt ttcgccgcca
     9181 gtggatggtc aggtgctaac accagacgca cttcatagtc gaacatcggc gaataatgca
     9241 ggccactgcg cggcaggata tcggaggtca tcaccagatc cagctcgccc tgttgcaacg
     9301 cgggctgcgg gtcaaacgtc acgcccgatt taaaatccat ctctacctgc ggccagttct
     9361 tatggaaatt ttctaacgcg ggcgtcagcc actgaataca gctatggcac tcaatggcaa
     9421 tgcgcagacg cgtctgctgc ggttcattgc aggcttgcag tgcctggcta atttgcggca
     9481 gcacctggtt tgccagttgc aacaggattt ctccctgcgg tgtaaagcgt agcggctggc
     9541 tcttacgcac aaatagccgg aagccaaggc gttgttccag atcgctaaac tggtgagaca
     9601 gggcggattg cgtctgatgc aacgtcgccg cagcggctgc gagagagccg cagttccgca
     9661 acgcttgtag cgttttcagg tgttttactt cgatcatgaa agtccttcac ttcggcatga
     9721 ataatttgcg cttgaggaat atacagtaac cgccaattat ggatgtgtaa acatctggac
     9781 ggctaaatac taaaaattcc ataaggggca tataatgaca attcttaatc acaccctcgg
     9841 tttccctcgc gttggcctgc gtcgcgagct gaaaaaagcg caagaaagtt attgggcggg
     9901 gaactccacg cgtgaagaac tgctgacagt agggcgtgaa ctgcgtgctc gtcactggga
     9961 tcaacaaaag caagcgggta tcgacctgct gccggtgggc gattttgcct ggtacgatca
    10021 tgttctgacc accagtctgc tgctgggtaa tgttccgcca cgtcatcaga acaaagacgg
    10081 ttcggtagat atcgacaccc tgttccgtat tggtcgtgga cgtgcgccga ctggcgaacc
    10141 tgcggcggca gcggaaatga ccaaatggtt taacaccaac tatcactaca tggtgccgga
    10201 attcgttaaa ggccaacagt tcaaactgac ctggacgcag ctgctggagg aagtggacga
    10261 ggcgctggcg ctgggccaca acgttaagcc tgtgctgctg gggccggtta cttacctgtg
    10321 gctggggaaa gtgaaaggtg aacagtttga ccgccttagc ctgctgaacg acattctgcc
    10381 ggtttatcag caagtgctgg cagaactggc gaaacgcggc atcgagtggg tacagattga
    10441 tgaacccgcg ctggtactgg aactaccaca ggcgtggctg gacgcataca aacccgctta
    10501 cgacgcgctc cagggacagg tgaaactgct gctgaccacc tattttgaag gcgtaacgcc
    10561 aaatctcgac acgattactg cgctgcctgt tcagggtctg catgttgacc tcgtacatgg
    10621 taaagatgac gttgctgaac tgcacaagcg cctgccttct gactggttgc tgtctgcggg
    10681 tctgatcaat ggtcgtaacg tctggcgcgc cgatcttacc gagaaatatg cgcaaattaa
    10741 ggacattgtc ggcaaacgtg atttgtgggt ggcatcttcc tgctcgttgc tgcacagccc
    10801 catcgacctg agcgtggaaa cgcgtcttga tgcagaagtg aaaagctggt ttgccttcgc
    10861 cctacaaaaa tgccatgaac tggcactgct gcgcgatgcg ctgaacagtg gtgacacggc
    10921 agctctggca gagtggagcg ccccgattca ggcacgtcgt cactctaccc gcgtacataa
    10981 tccggcggta gaaaagcgtc tggcggcgat caccgcccag gacagccagc gtgcgaatgt
    11041 ctatgaagtg cgtgccgaag cccagcgtgc gcgttttaaa ctgccagcgt ggccgaccac
    11101 cacgattggt tctttcccgc aaaccacgga aattcgtacc ctgcgtctgg atttcaaaaa
    11161 gggcaatctc gacgccaata actaccgcac gggcattgcg gaacatatca ggcaggccat
    11221 tgttgaacag gaacgtttgg gactggatgt gctggtacat ggcgaggccg agcgtaatga
    11281 catggtggaa tactttggcg agcacctcga cgggtttgtc tttacgcaaa acggttgggt
    11341 acagagctac ggttcccgct gcgtgaagcc accgattgtt attggtgacg ttagtcgccc
    11401 ggcaccgatt accgtggagt gggcgaagta tgcgcaatcg ctgaccgaca aaccggtgaa
    11461 agggatgctg acggggccgg tgaccatact ctgctggtcg ttcccgcgtg aagatgtcag
    11521 ccgtgaaacc atcgccaaac agattgcgct ggcgctgcgt gatgaggtgg ccgatctgga
    11581 agcggcaggg attggtatca tccagattga cgaaccggcg ctgcgcgaag gtttaccact
    11641 gcgtcgtagc gactgggacg cctatctcca gtggggcgta gaggccttcc gtatcaacgc
    11701 cgccgtggcg aaagatgaca cacaaatcca cactcacatg tgttattgcg agttcaacga
    11761 catcatggat tccattgcgg cgctggatgc agacgtcatc accatcgaaa cctcgcgttc
    11821 cgacatggag ttgctggagt cgtttgaaga gtttgattat ccgaatgaaa tcggtccagg
    11881 cgtctatgac attcactcgc caaacgtacc gagcgtggaa tggattgaag ccctgctgaa
    11941 gaaagcggca aaacgcattc cggcagagcg cctgtgggtc aacccagact gtggcctgaa
    12001 aacgcgcggc tggccagaaa cccgcgcggc actggcgaac atggtgcagg cggcgcagaa
    12061 tttgcgtctg gggtaaatcc aaaccgggtg gtaataccac ccggtctttt ctcattacag
    12121 cgacttcttc ccaccatact gcttaaacca ttccagcata cgctgccagc catctttcgc
    12181 agattcggca tgatagctcg ggcgataatc agcgttgaat gcatgcccgg catccgggta
    12241 gacgataatc tctgctttcg cattagcagc ccgcagcgcc tggcgcatcg tttcaacgct
    12301 ctcctgcgga atgctgttat cctgaccgcc atataagccg agaaccggcg cgttaagatc
    12361 ggttgcgata tcaacaggtt gtttcggtga attcagcgac ttatcgcccg tcagtttgcc
    12421 gtaccacgcc actgcggctt ttagctgtgg attatgcgcg gcatacagcc aggtgatacg
    12481 tccaccccag cagaatccgg tgatcattaa acgatgaaca tcgccgccgt tgcgggaagc
    12541 ccaactggcg acatgatcga gatcggccag cacctgcgag tcaggcactt ttgctaccag
    12601 accgctaagc aacgtgggga tatcggcaaa atcattcgga tcgccttcgc ggaagtaaag
    12661 ttcaggtgcg atagccagat acccctccag cgccagacgg cgacaaatgt cgcggatatg
    12721 ttcatgcacg ccaaaaattt cctgcactac aatgaccact ggcagtgggc catcgctttg
    12781 ctttggtctg gcatggtaag caggcatgtt atccccttgt gaagggatag aggtgaagcc
    12841 cgccacaatc gcgtcgtccg gggtctgaac gatggtcgaa gcgagaggcg atgcagcagg
    12901 tgcaaatcca gattgttgtg ttgttgccat ggtattctcc gtacccttat aaaaatgttg
    12961 cgcaatgtta actatagtca gcatgcaaca aatcacattg cctgaatcgg ctcatctttt
    13021 atgcagtcct gcagaatgaa gagtgattta tgtgatttgc atcacttttg gtgggtaaat
    13081 ttatgcaacg catttgcgtc atggtgatga gtatcacgaa aaaatgttaa acccttcggt
    13141 aaagtgtctt tttgcttctt ctgactaaac cgattcacag aggagttgta tatgtccaag
    13201 tctgatgttt ttcatctcgg cctcactaaa aacgatttac aaggggctac gcttgccatc
    13261 gtccctggcg acccggatcg tgtggaaaag atcgccgcgc tgatggataa gccggttaag
    13321 ctggcatctc accgcgaatt caccacctgg cgtgcagagc tggatggtaa acctgttatc
    13381 gtctgctcta ccggtatcgg cggcccgtct acctctattg ctgttgaaga gctggcacag
    13441 ctgggcattc gcaccttcct gcgtatcggt acaacgggcg ctattcagcc gcatattaat
    13501 gtgggtgatg tcctggttac cacggcgtct gtccgtctgg atggcgcgag cctgcacttc
    13561 gcaccgctgg aattcccggc tgtcgctgat ttcgaatgta cgactgcgct ggttgaagct
    13621 gcgaaatcca ttggcgcgac aactcacgtt ggcgtgacag cttcttctga taccttctac
    13681 ccaggtcagg aacgttacga tacttactct ggtcgcgtag ttcgtcactt taaaggttct
    13741 atggaagagt ggcaggcgat gggcgtaatg aactatgaaa tggaatctgc aaccctgctg
    13801 accatgtgtg caagtcaggg cctgcgtgcc ggtatggtag cgggtgttat cgttaaccgc
    13861 acccagcaag agatcccgaa tgctgagacg atgaaacaaa ccgaaagcca tgcggtgaaa
    13921 atcgtggtgg aagcggcgcg tcgtctgctg taattctctt ctcctgtctg aaggccgacg
    13981 cgttcggcct tttgtatttt tgcgtagcgc ctcgcaggaa atgcctttcc aactggacgt
    14041 ttgtacagca caattctatt ttgtgcgggt aagttgttgc gtcaggaggc gttgtggatt
    14101 tctcaatcat ggtttacgca gttattgcgt tggtgggtgt ggcaattggc tggctgtttg
    14161 ccagttatca acatgcgcag caaaaagccg agcaattagc tgaacgtgaa gagatggtcg
    14221 cggagttaag cgcggcaaaa caacaaatta cccaaagcga gcactggcgt gcagagtgcg
    14281 agttactcaa taacgaagtg cgcagcctgc aaagtattaa cacctctctg gaggccgatc
    14341 tgcgtgaagt aaccacgcgg atggaagccg cacagcaaca tgctgacgat aaaattcgcc
    14401 agatgattaa cagcgagcag cgcctcagtg agcagtttga aaacctcgcc aaccgtattt
    14461 ttgagcacag caatcgccgg gttgatgagc aaaaccgtca gagtctgaac agcctgttgt
    14521 cgccgctacg tgaacaactg gacggtttcc gccgtcaggt tcaggacagc ttcggtaaag
    14581 aagcacaaga acgccatacc ctgacccacg aaattcgcaa tctccagcaa ctcaacgcgc
    14641 aaatggccca ggaagcgatc aacctgacgc gcgcgctgaa aggcgacaat aaaacccagg
    14701 gcaactgggg cgaggtagta ttgacgcggg tgctggaggc ttccggtctg cgtgaagggt
    14761 atgaatatga aacccaggtc agcatcgaaa atgacgcccg ctcgcggatg cagccggatg
    14821 tcatcgtgcg gctgccgcag ggaaaagatg tggtgatcga cgccaaaatg acgctggtcg
    14881 cctatgaacg ctattttaac gccgaagacg actacacccg cgaaagcgcg ctacaggaac
    14941 atatcgcgtc ggtgcgtaac catatccgtt tgctgggacg caaagattat caacagctgc
    15001 cggggctgcg aactctggat tacgtgctga tgtttattcc cgttgaaccc gctttttcac
    15061 tggcgcttga ccgccagccg gagctgatca ccgaagcgtt gaaaaacaat atcatgctgg
    15121 ttagcccgac tacgctgctg gtggcgttgc gtactattgc caacctgtgg cgttatgagc
    15181 atcaaagccg caacgcccag caaatcgccg atcgtgccag caagctgtac gacaagatgc
    15241 gtttgttcat cgatgacatg tccgcgattg gtcaaagtct cgacaaagcg caggataact
    15301 accgccaggc aatgaaaaaa ctctcttcag gtcgcggaaa tgtgctggcg caggcagaag
    15361 cgtttcgcgg tttaggagta gaaattaaac gcgagattaa tccggatttg gctgaacagg
    15421 cggtgagcca ggatgaagag tatcgacttc ggtcggttcc ggagcagccg aatgatgaag
    15481 cttatcaacg cgatgatgaa tataatcagc agtcgcgcta gcccattagg ggtagttaag
    15541 ccgggtagaa atctagggca tcgacgccca atctgttaca cttctggaac aattttttga
    15601 tgagcaggca ttgagatggt ggataagtca caagaaacga cgcactttgg ttttcagacc
    15661 gtcgcgaagg aacaaaaagc ggatatggtc gcccacgttt tccattccgt ggcatcaaaa
    15721 tacgatgtca tgaatgattt gatgtcattt ggtattcatc gtttgtggaa gcgattcacg
    15781 attgattgca gcggcgtacg ccgtgggcag accgtgctgg atctggctgg tggcaccggc
    15841 gacctgacag cgaaattctc ccgcctggtc ggagaaactg gcaaagtggt ccttgctgat
    15901 atcaatgaat ccatgctcaa aatgggacgc gagaagctgc gtaatatcgg tgtgattggc
    15961 aacgttgagt atgttcaggc gaacgctgag gcgctgccgt tcccggataa cacctttgat
    16021 tgcatcacca tttcgtttgg tctgcgtaac gtcaccgaca aagataaagc actgcgttca
    16081 atgtatcgcg tgctgaaacc cggcggccgc ctgctggtgc ttgagttctc gaagccaatt
    16141 atcgagccgc tgagcaaagc ctatgatgca tactccttcc atgtgctgcc gcgtattggc
    16201 tcactggtcg cgaacgacgc cgacagctac cgttatctgg cagaatccat ccgtatgcat
    16261 cccgatcagg ataccctgaa agccatgatg caggatgccg gattcgaaag tgtcgactac
    16321 tacaatctga cggcgggggt tgtggcgctg catcgtggtt ataagttctg acaggagacc
    16381 ggaaatgcct tttaaacctt tagtgacggc aggaattgaa agtctgctca acaccttcct
    16441 gtatcgctca cccgcgctga aaacagcccg ctcgcgtctg ctgggtaaag tattgcgcgt
    16501 ggaggtaaaa ggcttttcga cgtcattgat tctggtgttc agcgaacgcc aggttgatgt
    16561 actgggcgaa tgggcaggcg atgctgactg caccgttatc gcctacgcca gtgtgttgcc
    16621 gaaacttcgc gatcgccagc agcttaccgc actgattcgc agtggtgagc tggaagtgca
    16681 gggcgatatt caggtggtgc aaaacttcgt tgcgctggca gatctggcag agttcgaccc
    16741 tgcggaactg ctggcccctt ataccggtga tatcgccgct gaaggaatca gcaaagccat
    16801 gcgcggaggc gcaaagttcc tgcatcacgg cattaagcgc cagcaacgtt atgtggcgga
    16861 agccattact gaagagtggc gtatggcacc cggtccgctt gaagtggcct ggtttgcgga
    16921 agagacggct gccgtcgagc gtgctgttga tgccctgacc aaacggctgg aaaaactgga
    16981 ggctaaatga cgccaggtga agtacggcgc ctatatttca tcattcgcac ttttttaagc
    17041 tacggacttg atgaactgat ccccaaaatg cgtatcaccc tgccgctacg gctatggcga
    17101 tactcattat tctggatgcc aaatcggcat aaagacaaac ttttaggtga gcgactacga
    17161 ctggccctgc aagaactggg gccggtttgg atcaagttcg ggcaaatgtt atcaacccgc
    17221 cgcgatcttt ttccaccgca tattgccgat cagctggcgt tattgcagga caaagttgct
    17281 ccgtttgatg gcaagctggc gaagcagcag attgaagctg caatgggcgg cttgccggta
    17341 gaagcgtggt ttgacgattt tgaaatcaag ccgctggctt ctgcttctat cgcccaggtt
    17401 cataccgcgc gattgaaatc gaatggtaaa gaggtggtga ttaaagtcat ccgcccggat
    17461 attttgccgg tcattaaagc ggatctgaaa cttatctacc gtctggctcg ctgggtgccg
    17521 cgtttgctgc cggatggtcg ccgtctgcgc ccaaccgaag tggtgcgcga gtacgaaaag
    17581 accttgattg atgaactgaa tttgctgcgg gaatctgcca acgccattca gcttcggcgc
    17641 aattttgaag acagcccgat gctctacatc ccggaagttt accctgacta ttgtagtgaa
    17701 gggatgatgg tgatggagcg catttacggc attccggtgt ctgatgttgc ggcgctggag
    17761 aaaaacggca ctaacatgaa attgctggcg gaacgcggcg tgcaggtgtt cttcactcag
    17821 gtctttcgcg acagcttttt ccatgccgat atgcaccctg gcaacatctt cgtaagctat
    17881 gaacacccgg aaaacccgaa atatatcggc attgattgcg ggattgttgg ctcgctaaac
    17941 aaagaagata aacgctatct ggcagaaaac tttatcgcct tctttaatcg cgactatcgc
    18001 aaagtggcag agctacacgt cgattcaggc tgggtgccac cagataccaa cgttgaagag
    18061 ttcgaatttg ccattcgtac ggtctgtgaa cctatctttg agaaaccgct ggccgaaatt
    18121 tcgtttggac atgtactgtt aaatctgttt aatacggcgc gtcgcttcaa tatggaagtg
    18181 cagccgcaac tggtgttact ccagaaaacc ctgctctatg tcgaaggggt aggacgccag
    18241 ctttatccgc agctcgattt atggaaaaca gcgaagcctt tcctggagtc gtggattaaa
    18301 gatcaggtcg gtattcctgc gctggtgaga gcatttaaag aaaaagcgcc gttctgggtc
    18361 gaaaaaatgc cagaactgcc tgaattggtt tacgacagtt tgcgccaggg caagtattta
    18421 cagcacagtg ttgataagat tgcccgcgag cttcagtcaa atcatgtacg tcagggacaa
    18481 tcgcgttatt ttctcggaat tggcgctacg ttagtattaa gtggcacatt cttgttggtc
    18541 agccgacctg agtgggggct gatgcccggc tggttaatgg caggtggtct gatcgcctgg
    18601 tttgtcggtt ggcgcaaaac acgctgattt tttcatcgct caaggcgggc cgtgtaacgt
    18661 ataatgcggc tttgtttaat catcatctac cacagaggaa catgtatggg tggtatcagt
    18721 atttggcagt tattgattat tgccgtcatc gttgtactgc tttttggcac caaaaagctc
    18781 ggctccatcg gttccgatct tggtgcgtcg atcaaaggct ttaaaaaagc aatgagcgat
    18841 gatgaaccaa agcaggataa aaccagtcag gatgctgatt ttactgcgaa aactatcgcc
    18901 gataagcagg cggatacgaa tcaggaacag gctaaaacag aagacgcgaa gcgccacgat
    18961 aaagagcagg tgtaatccgt gtttgatatc ggttttagcg aactgctatt ggtgttcatc
    19021 atcggcctcg tcgttctggg gccgcaacga ctgcctgtgg cggtaaaaac ggtagcgggc
    19081 tggattcgcg cgttgcgttc actggcgaca acggtgcaga acgaactgac ccaggagtta
    19141 aaactccagg agtttcagga cagtctgaaa aaggttgaaa aggcgagcct cactaacctg
    19201 acgcccgaac tgaaagcgtc gatggatgaa ctacgccagg ccgcggagtc gatgaagcgt
    19261 tcctacgttg caaacgatcc tgaaaaggcg agcgatgaag cgcacaccat ccataacccg
    19321 gtggtgaaag ataatgaagc tgcgcatgag ggcgtaacgc ctgccgctgc acaaacgcag
    19381 gccagttcgc cggaacagaa gccagaaacc acgccagagc cggtggtaaa acctgctgcg
    19441 gacgctgaac cgaaaaccgc tgcaccttcc ccttcgtcga gtgataaacc gtaaacatgt
    19501 ctgtagaaga tactcaaccg cttatcacgc atctgattga gctgcgtaag cgtctgctga
    19561 actgcattat cgcggtgatc gtgatattcc tgtgtctggt ctatttcgcc aatgatatct
    19621 atcacctggt atccgcgcca ttgatcaagc agttgccgca aggttcaacg atgatcgcca
    19681 ccgacgtggc ctcgccgttc tttacgccga tcaagctgac ctttatggtg tcgctgattc
    19741 tgtcagcgcc ggtgattctc tatcaggtgt gggcatttat cgccccagcg ctgtataagc
    19801 atgaacgtcg cctggtggtg ccgctgctgg tttccagctc tctgctgttt tatatcggta
    19861 tggcattcgc ctactttgtg gtctttccgc tggcatttgg cttccttgcc aataccgcgc
    19921 cggaaggggt gcaggtatcc accgacatcg ccagctattt aagcttcgtt atggcgctgt
    19981 ttatggcgtt tggtgtctcc tttgaagtgc cggtggcaat tgtgctgctg tgctggatgg
    20041 ggattacctc gccagaagac ttacgcaaaa aacgcccgta tgtgctggtt ggtgcattcg
    20101 ttgtcgggat gttgctgacg ccgccggatg tcttctcgca aacgctattg gcgatcccga
    20161 tgtactgtct gtttgaaatc ggtgtcttct tctcacgctt ttacgttggt aaagggcgaa
    20221 atcgggaaga ggaaaacgac gctgaagcag aaagcgaaaa aactgaagaa taaattcaac
    20281 cgcccgtcag ggcggttgtc atatggagta caggatgttt gatatcggcg ttaatttgac
    20341 cagttcgcaa tttgcgaaag accgtgatga tgttgtagcg cgcgcttttg acgcgggagt
    20401 taatgggcta ctcatcaccg gcactaacct gcgtgaaagc cagcaggcgc aaaagctggc
    20461 gcgtcagtat tcgtcctgtt ggtcaacggc gggcgtacat cctcacgaca gcagccagtg
    20521 gcaagctgcg actgaagaag cgattattga gctggccgcg cagccagaag tggtggcgat
    20581 tggtgaatgt ggtctcgact ttaaccgcaa cttttcgacg ccggaagagc aggaacgcgc
    20641 ttttgttgct cagctacgca ttgccgcaga attaaacatg ccggtattta tgcactgtcg
    20701 cgatgcccac gagcggttta tgacattgct ggagccgtgg ctggataaac tgcctggtgc
    20761 ggttcttcat tgctttaccg gcacacgcga agagatgcag gcgtgcgtgg cgcgtggaat
    20821 ttatatcggc attaccggtt gggtttgcga tgaacgacgc gggctggagc tgcgggaatt
    20881 gttgccgttg attccggcgg agaaattgct gatcgaaact gatgcgccgt atctgctccc
    20941 gcgcgatctc acgccaaagc catcatcccg gcgcaacgag ccagcccatc tgccccatat
    21001 tttgcaacgt attgcgcact ggcgtggaga agatgccgca tggctggctg ccaccacgga
    21061 tgctaatgtc aaaacactgt ttgggattgc gttttagagt ttgcggaact cggtgttctt
    21121 cacactgtgc ttaatctctt tattaataag attaagcaat agcatggagc gagcctcacc
    21181 atcgggttcg gtgaaaatgg cctgaaagcc ttcgaacgcg ccttcggtaa taatcacctt
    21241 atcacccgga taaggggttg ccggatcgac aatgtctttc ggtttatata ccgatagctg
    21301 atgaatcacc gccgatggga ctatcgctgg cgacgcgcca aagcgcacga agtggctgac
    21361 accgcgggtc gcgttgatag tcgtggtatg aatcacttct ggatcaaatt cgacaaacag
    21421 gtagttggga aacaatggct cactgactgc agtacgtttt ccacgcacga ttttttccag
    21481 ggtgatcatc ggtgccaggc aattcacagc ctgtctttcg aggtgttcct gggcacgttg
    21541 aagttgcccg cgcttgcagt acagtaaata ccaggattgc ataatgactc ttatccgttt
    21601 aatcggggcg caaggatagc aaaagcttta cgctaagtta attatattcc ccggtttgcg
    21661 ttataccgtc agagttcacg ctaatttaac aaatttacag catcgcaaag atgaacgccg
    21721 tataatgggc gtagattaag aggctacaat ggacgccatg aaatataacg atttacgcga
    21781 cttcttgacg ctgcttgaac agcagggtga gctaaaacgt atcacgctcc cggtggatcc
    21841 gcatctggaa atcactgaaa ttgctgaccg cactttgcgt gccggtgggc ctgcgctgtt
    21901 gttcgaaaac cctaaaggct actcaatgcc ggtgctgtgc aacctgttcg gtacgccaaa
    21961 gcgcgtggcg atgggcatgg ggcaggaaga tgtttcggcg ctgcgtgaag ttggtaaatt
    22021 attggcgttt ctgaaagagc cggagccgcc aaaaggtttc cgcgacctgt ttgataaact
    22081 gccgcagttt aagcaagtat tgaacatgcc gacaaagcgg ctgcgtggtg cgccctgcca
    22141 acaaaaaatc gtctctggcg atgacgtcga tctcaatcgc attcccatta tgacctgctg
    22201 gccggaagat gccgcgccgc tgattacctg ggggctgaca gtgacgcgcg gcccgcataa
    22261 agagcggcag aatctgggca tttatcgcca gcagctgatt ggtaaaaaca aactgattat
    22321 gcgctggctg tcgcatcgcg gcggcgcgct ggattatcag gagtggtgtg cggcgcatcc
    22381 gggcgaacgt ttcccggttt ctgtggcgct gggtgccgat cccgccacga ttctcggtgc
    22441 agtcaccccc gttccggata cactttcaga gtatgcgttt gccggattgc tgcgcggtac
    22501 caagaccgaa gtggtgaagt gtatctccaa tgaccttgaa gtgcccgcca gtgcggagat
    22561 tgtgctggaa gggtatatcg aacaaggtga aacggcgccg gaagggccgt atggcgacca
    22621 caccggttac tataacgaag tcgatagttt cccggtattt accgtgacgc atattaccca
    22681 gcgtgaagat gcgatttacc attccaccta taccgggcgt ccgccagatg agcccgcggt
    22741 actgggcgtg gcgttgaacg aagtgtttgt accgattctg caaaaacagt tcccggaaat
    22801 tgtcgatttt tacctgccgc cggaaggctg ttcttatcgt ctggcggtag tgacgatcaa
    22861 aaaacagtac gccggacacg cgaagcgcgt catgatgggc gtctggtcgt tcttacgcca
    22921 gtttatgtac actaaatttg tgatcgtttg cgatgatgac gtcaacgcac gcgactggaa
    22981 cgatgtgatt tgggcgatta ccacccgtat ggacccggcg cgggatactg ttctggtaga
    23041 aaatacgcct attgattatc tggattttgc ctcgcctgtc tccgggctgg gttcaaaaat
    23101 ggggctggat gccacgaata aatggccggg ggaaacccag cgtgaatggg gacgtcccat
    23161 caaaaaagat ccagatgttg tcgcgcatat tgacgccatc tgggatgaac tggctatttt
    23221 taacaacggt aaaagcgcct gatgcgcgtt tgttttgccc tatttatcga tccgacagag
    23281 aaagcgcatg acaaccttaa gctgtaaagt gacctcggta gaagctatca cggataccgt
    23341 atatcgtgtc cgcatcgtgc cagacgcggc cttttctttt cgtgctggtc agtatttgat
    23401 ggtagtgatg gatgagcgcg acaaacgtcc gttctcaatg gcctcgacgc cggatgaaaa
    23461 agggtttatc gagctgcata ttggcgcttc tgaaatcaac ctttacgcga aagcagtcat
    23521 ggaccgcatc ctcaaagatc atcaaatcgt ggtcgatatt ccccacggag aagcgtggct
    23581 gcgcgatgat gaagagcgtc cgatgatttt gattgcgggc ggcaccgggt tctcttatgc
    23641 ccgctcgatt ttgctgacag cgttggcgcg taacccaaac cgtgatatca ccatttactg
    23701 gggcgggcgt gaagagcagc atctgtatga tctctgcgag cttgaggcgc tttcgttaaa
    23761 gcaccctggt ctgcaagtgg tgccggtggt tgaacaaccg gaagcaggct ggcgtgggcg
    23821 tactggtacc gtgttaacgg cggtattgca ggatcatggt acgctggcag agcatgatat
    23881 ctatattgcc ggacgttttg agatggcgaa aattgcccgt gacctgtttt gcagtgagcg
    23941 taatgcgcgg gaagatcgcc tgtttggcga tgcgtttgca tttatctgag atataaaaaa
    24001 acccgcccct gacaggcggg aagaacggca actaaaactt tattcagtgg catttagatc
    24061 tatgtcgcat ccggcaatcg gtgcacgatg cctgatgcga cgctggcgcg tcttatcagg
    24121 cctacattgg tgcaggatcg gtagaccgga taaggcgttc acgccgcatc cggcaagtgg
    24181 ttaaacccgc tcaaacaccg tcgcaatacc ctgacccaga ccgatacaca tcgtcgccag
    24241 accaaactga acgtctttgc gttccatcag attcagcagc gtggtgctga tacgcgcacc
    24301 ggaacagccc agcggatgac ccagcgcgat cgcaccaccg ttgaggttga tcttctcgtc
    24361 aatctgctca atcagtccca gatctttaat acatggcagg atctgcgcgg caaaggcttc
    24421 gttcatctca aacacgccga tatcgctggc agaaagcccc gcttttttca gcgccagctt
    24481 cgaggccgga accgggccgt aacccataat cgatgggtca caaccaacga ccgccatcga
    24541 acgcacacga gcgcgcggct taagacctaa ttcatgggcg cggctttcac tcatcaccag
    24601 catggcagct gcgccatcgg aaagtgcaga agatgtgccc gccgttaccg taccgtttac
    24661 tggatcaaac gccggacgca gcgtggcgag ggcttccacg gtggtttccg ggcgaatcac
    24721 ttcgtcgtaa ttaaactgct tcaggacgcc gtcggcatcg tgaccaccag ttgggatgat
    24781 ttcattttta aatgcggccg actgcgtggc ggcccaggcg cgggcgtgtg accgcgcggc
    24841 aaaggcatcc tgcatttcac ggctgatacc gtgcatacgc gccagcattt ctgctgttaa
    24901 gcccatcatg cccgccgctt tggcgacatt gcggctcagg ccggggtgaa aatcgacgcc
    24961 gtgactcatc ggcacatggc ccatatgctc cacgccgcca accagacatg cctgcgcatc
    25021 gccggtcata atcatccgtg ctgcgtcatg cagtgcctgc atggatgaac cacacaagcg
    25081 attgacggta accgccggga cagagtgtgg tacttctgcc agcagcgccg cgttacgggc
    25141 gatgttaaaa ccctgctcca gcgtctgctg cacacaaccc cagtaaatat cgtcgagggc
    25201 cgccgcttcc agcgccgggt tacgcgccag caggctacgc attaaatgag cggagagatc
    25261 ttctgcacgc acgttacgaa aagcaccgcc cttcgaacgg cccatcgggg tgcgaattgc
    25321 atcgacaatg acaacctgtt ccattgtgac tccttaagcc gttttcaggt cgccaaccgg
    25381 acgggctggc tcaaccggag gatagtacgg ttcgttatga cgcgctttat tacgcagacc
    25441 ttccggcact tcatacagcg ggccgaggtg ctgatattgc tgtgccatat cgaggtattt
    25501 tgcgctaccg agggtgtcca gccagcggaa cgcgccgccg tggaacggag ggaagcccag
    25561 gccgtagacc agcgccatat ccgcttccgc cggagtggcg ataatgcctt cctccagaca
    25621 gcgcaccact tcgttgacca tcgggatcat catgcgggcg ataatctcct cttcgctgaa
    25681 atcgcgcttc ggctggctca cttctgccag caggtcttca acggcggcgt cttcttcttt
    25741 cttcggctta cctttgctgt cttctttata acgccagaaa ccgaggccgt tcttctgacc
    25801 aaagcggttg gcatcaaaca gcgcgtcgat ggcatcgcgg taatctttct gcatcctctg
    25861 cgggaagcct gctgccatga cagcctgggc atgatgcgcg gtgtcaatgc ccacgacatc
    25921 cagcagatat gccggaccca tcggccagcc aaactgtttt tccatcactt tgtcgatctt
    25981 gcggaaatcc gcgccgtcgc gcagcagctg gctgaaaccg gcgaaatacg ggaacagcac
    26041 gcggttaaca aagaagccgg ggcagtcgtt aaccacaatc ggcgtcttgc ccatcttgct
    26101 cgcccaggcg acaactttcg cgatggtttc gtcggagctt ttctcgccgc gaataatttc
    26161 taccaacggc attcggtgga ccgggttaaa gaagtgcatc ccgcagaagt tttccgggcg
    26221 tttcagcgcg ttggccagtt cgctgatagg aatggttgaa gtgttagacg ccagcacggt
    26281 atcctggcgt actttttgtt cggtttctgc cagtacggct tttttcactt tcgggttttc
    26341 aacaaccgct tctaccacaa tatccacgcg gtcaaatccg gcgtagtcga gcgttgggtg
    26401 gattgtggag atcacgccag ccagtttcag accatcgatc ttgccgcgct caagctgctt
    26461 gttcagcagt ttcgcggctt cggtcatgcc gagggttaac gacttgtcgt tgatatcttt
    26521 catgacaacc ggcacgcctt tccacgcaga ctggtaagcg atgccgccgc ccataatgcc
    26581 tgcacccagc accgcggcct gtttcggggt ttcaacgtct ttggtgagtt tcttcgcttt
    26641 gccttttaca tattgatcgt taaggaaaat gccgaccagt gcgcgggctt cgttggtatg
    26701 cgccagcggg acaaaacttt tgttttccag gtttaaggct tcttcacgac caaaacgggc
    26761 cgcagcttca atggttttta ctgcggtgat gggggccgga taatgtttcc ccgctgtttg
    26821 tgcgaccatc cctttagcga tggtgaagct catggtggct tcaatcttgc tcagttttag
    26881 tggttccagc ttcggctgac gttttgcttt ccagtcgagg tcgccgttaa tggcctggcg
    26941 taaaaccgcc tttgcgcctt caaccagttt ttctgctttg actacgccat ccaccagacc
    27001 gattttcagc gcctgatccg cgccgacatc tttaccggcg gcaatgattt ccagcgcact
    27061 gtctgcgccc agcatacgtg gcatacgtac agaaccgcca aagcccggca tgatgcccag
    27121 tttggtttcc ggcagaccga tgcgcagatc cggcgtcgcc agacgataat cggtcgccag
    27181 cacgcattcg cagccaccgc ccagcgcata gccgttgacg gcagcaatgg tcggcaccgg
    27241 cagatcttcc aggcgattaa acacgctatt ggcaaagtgc agccactgac ttaactgttc
    27301 ttcaggaacg aggaacaggg acaaaaattc ggtgatatca gcaccgacga taaaggctgc
    27361 tttgttcgaa cgcagcagca gcccttttag atctgattgc tgttccagca cgccgatggc
    27421 ctcgccgagg ctggcgacgg tcgcagtgtc gagtttatta actgaacctg gggcatcaaa
    27481 taccagttcg gcaatgccat cttccagcca gtcaaggtac agggtgtcgc ctttgtaaag
    27541 catgtcagtc tcctgaatcc gcaaggtgat ctggtcgtac cagatgcatc gaagtgtgta
    27601 ttttgtgtta aaaatatgca aataaaagat taaagaaatg ccgatctgat cacgctcggc
    27661 agaaatcacg ctctggatga acgatgtgct aagatgcgga gacttaaggt caaaaaaaca
    27721 gaagggtaaa aaatggaatc actggcctca ctctataaaa atcatatagc taccttacag
    27781 gaacggactc gcgatgcgct ggcgcgcttc aagctggatg cgttacttat tcactccggc
    27841 gaactgttca acgtttttct cgacgatcat ccctatccgt ttaaagtgaa cccgcaattc
    27901 aaagcgtggg tgccggtaac tcaggtgcca aactgctggc tgctggtgga tggcgtgaac
    27961 aagccgaaac tgtggttcta tctgccggtt gattactggc acaacgtcga accgctgccg
    28021 acctctttct ggactgaaga cgtggaagtg atcgcgctgc cgaaagccga tggcattggt
    28081 agcctgctgc ctgctgcgcg cgggaatatc ggttatatcg gtccggtgcc ggaacgtgcg
    28141 ctgcaactgg gtattgaggc cagcaatatc aacccgaaag gggtgatcga ctacctgcat
    28201 tactaccgct ccttcaaaac cgagtacgaa ctggcctgta tgcgtgaagc gcagaaaatg
    28261 gcggtcaacg gtcatcgcgc ggcagaagaa gcgttccgtt ctggcatgag cgagttcgat
    28321 atcaacattg cctatctgac cgcgaccggt catcgtgata ccgacgtacc ttacagcaac
    28381 attgtggcgc tcaacgaaca cgctgcggtg ttgcattaca ccaaactgga ccaccaggca
    28441 ccggaagaga tgcgcagctt cctgctggat gccggggccg aatataacgg ctatgccgct
    28501 gacctgaccc gtacctggtc ggcaaaaagt gacaacgatt acgcacagct ggtgaaagac
    28561 gtaaatgatg aacaactggc gctgatcgcg accatgaaag ctggcgttag ctatgtggat
    28621 taccacatcc agttccatca gcgcatcgcc aaactgctgc gtaaacatca aatcatcacc
    28681 gatatgagtg aagaagcgat ggtcgaaaac gatctcaccg gaccgtttat gccgcacggt
    28741 atcggccatc cgctgggcct gcaggtgcat gacgtcgccg gttttatgca ggatgatagc
    28801 ggtacgcacc tcgcggcacc ggcaaaatat ccgtacctgc gctgcacccg tattctccag
    28861 ccgggcatgg tgttaaccat cgaaccgggt atctacttca ttgaatcgct actggcaccg
    28921 tggcgtgaag ggcagttcag caagcacttc aactggcaga aaattgaagc actgaaaccg
    28981 ttcggcggca ttcgtatcga agacaacgtg gtgatccacg aaaataacgt ggaaaacatg
    29041 acccgggatc tgaaactggc gtgatggaaa gctggttaat tcctgcggca ccggtcacgg
    29101 tcgttgaaga gatcaaaaag agccgcttca taacgatgtt ggcgcatacc gatggcgttg
    29161 aggcggcgaa agcgtttgtt gaatcggtgc gggcagaaca ccccgatgcc cgccaccatt
    29221 gcgtggcgtg ggtggcgggt gcgccggatg attcacaaca gctgggtttc tctgacgacg
    29281 gggagccggc gggaacggca ggtaaaccga tgctcgccca gctaatgggc agcggcgttg
    29341 gggaaattac cgctgtggtg gtgcgctact acggcggcat attgcttggc accggtgggt
    29401 tagtgaaagc gtatggcggc ggcgtgaatc aggcgctgcg ccagctaacg acccaacgca
    29461 agacgccatt aaccgaatat actttgcaat gtgaatatca tcagttaacc ggcattgaag
    29521 cgttgctggg gcagtgtgac ggcaaaatta tcaacagtga ttatcaggca ttcgttctgc
    29581 tgcgggtggc gcttccggcg gcgaaagtgg ctgaattttc cgcaaagctg gcggatttta
    29641 gccgtggttc attgcaattg ttagcgattg aagaataatc cccacttcgt tttgcagaac
    29701 taaggaagcg gcagagatgc attttcgcgc cattacccga atcgttggac tactggtcat
    29761 cttattttca gggaccatga ttatccctgg gctggtagca ctcatctacc gggatggagc
    29821 gggccgcgct tttacccaga ccttttttgt cgccctcgcc attggctcta tgctgtggtg
    29881 gccgaaccgc aaagagaaag gcgaacttaa atcccgtgag gggtttctga tagtggtgct
    29941 gttctggacc gtgctgggta gcgtcggtgc gctccctttt atcttctcgg aaagcccgaa
    30001 cctcacgatt accgatgcgt tttttgaatc tttctctggc ctgaccacca ccggggccac
    30061 tacgctggtg gggctggatt cgctccctca cgccatcctc ttttatcgcc agatgctgca
    30121 atggtttggc gggatgggga tcatcgtgtt agcggttgcg atactgccta tcctcggcgt
    30181 gggtgggatg cagctctatc gcgcagaaat gcccggcccg ctgaaagata acaaaatgcg
    30241 cccgcgaatt gcggaaacgg cgaaaaccct gtggttgatt tatgtcttgc tgaccgtcgc
    30301 ctgtgcgctg gcgttgtggt ttgccggaat ggatgccttt gatgccatcg gccatagctt
    30361 tgcgactatc gctattggcg gcttctcgac acatgatgcc agtatcggtt atttcgatag
    30421 cccgactatt aacactatca ttgctatctt cctgctgatc tccggctgta actacggtct
    30481 gcacttttca ctgttaagtg ggcgtagtct gaaggtttat tggcgcgatc cggaatttcg
    30541 catgtttatc ggcgtacagt ttacgctggt ggttatttgt accctcgtac tgtggtttca
    30601 taatgtctac agttcggcgc tgatgacaat taaccaggcg tttttccagg tggtgtcgat
    30661 ggcgacaacc gccgggttta cgactgacag cattgcccgc tggccgctct ttttgccggt
    30721 actgctttta tgttcggctt ttatcggcgg ttgtgccggg tcaacgggcg gtggcctgaa
    30781 agtgatccgc atcctgctgc tgtttaagca ggggaaccgt gagctgaaac gactggtgca
    30841 tccgaacgcc gtctatagca ttaagctggg gaatcgcgca ctgccggaac gtatcctcga
    30901 agccgtttgg ggatttttct ccgcctatgc attggtgttt attgtcagta tgctggcgat
    30961 tatcgccacg ggcgtggatg acttttctgc ctttgcctca gttgttgcga cattgaataa
    31021 cctggggcca gggcttggcg tggttgctga taactttacc agtatgaacc cggtggctaa
    31081 atggatcctg attgccaaca tgctgtttgg tcgtctcgag gtctttacat tgctggtgct
    31141 ctttaccccg actttctggc gtgaatgatg gagtaatacg tgaaaacatt aattcttttc
    31201 tcaacaaggg acggacaaac gcgcgagatt gcctcctacc tggcttcgga actgaaagaa
    31261 ctggggatcc agacggatgt cgccaatgtg caccgcattg aagaaccaca atgggaaaat
    31321 tatgaccgtg tggtcattgg tgcttctatt cgctatggtc actaccactc tgcgttccag
    31381 gaatttgtca aaaaacatgc gacgcgactg aattcgatgc cgagcgcctt ttattccgta
    31441 aacctggtgg cgcgcaaacc ggagaagcgt actccacaga ccaacagcta cgcgcggaag
    31501 tttctgatga actcgcaatg gcgtcccgat cgctgcgcgg tcattgccgg ggcgctgcgt
    31561 tacccacgtt atcgctggta cgaccgtttt atgatcaagc tgattatgaa gatgtcaggc
    31621 ggtgaaacgg atacgcgcaa agaagttgtc tataccgatt gggagcaggt ggcgaatttc
    31681 gcccgagaaa tcgcccattt aaccgacaaa ccgacgctga aataagcaca aagaa
//
LOCUS       DAZKHF020000022        31308 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2201S31241
            SAMN43267800-rid25106413.denovo.022, whole genome shotgun sequence.
ACCESSION   DAZKHF020000022 DAZKHF020000000
VERSION     DAZKHF020000022.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267800
            Sequence Read Archive: SRR30307263
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 31308)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 31308)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 31308)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350581.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350581.2
            Genome Coverage       :: 151x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:21:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,652
            CDSs (total)                      :: 5,557
            Genes (coding)                    :: 5,320
            CDSs (with protein)               :: 5,320
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 237
            CDSs (without protein)            :: 237
            Pseudo Genes (ambiguous residues) :: 0 of 237
            Pseudo Genes (frameshifted)       :: 102 of 237
            Pseudo Genes (incomplete)         :: 130 of 237
            Pseudo Genes (internal stop)      :: 52 of 237
            Pseudo Genes (multiple problems)  :: 41 of 237
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..31308
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267800-rid25106413.denovo.022"
                     /serotype="O 26:H11"
                     /isolate="2201S31241"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-01"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            complement(118..3267)
                     /gene="acrB"
                     /locus_tag="ACHOSC_RS04390"
                     /old_locus_tag="pgaptmp_000878"
     CDS             complement(118..3267)
                     /gene="acrB"
                     /locus_tag="ACHOSC_RS04390"
                     /old_locus_tag="pgaptmp_000878"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease AcrB"
                     /protein_id="HGU4546866.1"
                     /translation="MPNFFIDRPIFAWVIAIIIMLAGGLAILKLPVAQYPTIAPPAVT
                     ISASYPGADAKTVQDTVTQVIEQNMNGIDNLMYMSSNSDSTGTVQITLTFESGTDADI
                     AQVQVQNKLQLAMPLLPQEVQQQGVSVEKSSSSFLMVVGVINTDGTMTQEDISDYVAA
                     NMKDAISRTSGVGDVQLFGSQYAMRIWMNPNELNKFQLTPVDVITAIKAQNAQVAAGQ
                     LGGTPPVKGQQLNASIIAQTRLTSTEEFGKILLKVNQDGSRVLLRDVAKIELGGENYD
                     IIAEFNGQPASGLGIKLATGANALDTAAAIRAELAKMEPFFPSGLKIVYPYDTTPFVK
                     ISIHEVVKTLVEAIILVFLVMYLFLQNFRATLIPTIAVPVVLLGTFAVLAAFGFSINT
                     LTMFGMVLAIGLLVDDAIVVVENVERVMAEEGLPPKEATRKSMGQIQGALVGIAMVLS
                     AVFVPMAFFGGSTGAIYRQFSITIVSAMALSVLVALILTPALCATMLKPIAKGDHGEG
                     KKGFFGWFNRMFEKSTHHYTDSVGGILRSTGRYLVLYLIIVVGMAYLFVRLPSSFLPD
                     EDQGVFMTMVQLPAGATQERTQKVLNEVTHYYLTKEKNNVESVFAVNGFGFAGRGQNT
                     GIAFVSLKDWADRPGEENKVEAITMRATRAFSQIKDAMVFAFNLPAIVELGTATGFDF
                     ELIDQAGLGHEKLTQARNQLLAEAAKHPDMLTSVRPNGLEDTPQFKIDIDREKAQALG
                     VSINDINTTLGAAWGGSYVNDFIDRGRVKKVYVMSEAKYRMLPDDIGDWYVRAADGQM
                     VPFSAFSSSRWEYGSPRLERYNGLPSMEILGQAAPGKSTGEAMELMEQLASKLPTGVG
                     YDWTGMSYQERLSGNQAPSLYAISLIVVFLCLAALYESWSIPFSVMLVVPLGVIGALL
                     AATFRGLTNDVYFQVGLLTTIGLSAKNAILIVEFAKDLMDKEGKGLIEATLDAVRMRL
                     RPILMTSLAFILGVMPLVISTGAGSGAQNAVGTGVMGGMVTATVLAIFFVPVFFVVVR
                     RRFSRKNEDIEHSHTVDHH"
     gene            complement(3290..4483)
                     /gene="acrA"
                     /locus_tag="ACHOSC_RS04395"
                     /old_locus_tag="pgaptmp_000879"
     CDS             complement(3290..4483)
                     /gene="acrA"
                     /locus_tag="ACHOSC_RS04395"
                     /old_locus_tag="pgaptmp_000879"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter periplasmic
                     adaptor subunit AcrA"
                     /protein_id="HGU4546867.1"
                     /translation="MNKNRGFTPLAVVLMLSGSLALTGCDDKQAQQGGQQMPAVGVVT
                     VKTEPLQITTELPGRTSAYRIAEVRPQVSGIILKRNFKEGSDIEAGVSLYQIDPATYQ
                     ATYDSAKGDLAKAQAAANIAQLTVNRYQKLLGTQYISKQEYDQALADAQQANAAVTAA
                     KAAVETARINLAYTKVTSPISGRIGKSNVTEGALVQNGQATALATVQQLDPIYVDVTQ
                     SSNDFLRLKQELANGTLKQENGKAKVSLITSDGIKFPQDGTLEFSDVTVDQTTGSITL
                     RAIFPNPDHTLLPGMFVRARLEEGLNPNAILVPQQGVTRTPRGDATVLVVGADDKVET
                     RPIVASQAIGDKWLVTEGLKAGDRVVISGLQKVRPGVQVKAQEVTADNNQQAASGAQP
                     EQSKS"
     gene            4625..5272
                     /gene="acrR"
                     /locus_tag="ACHOSC_RS04400"
                     /old_locus_tag="pgaptmp_000880"
     CDS             4625..5272
                     /gene="acrR"
                     /locus_tag="ACHOSC_RS04400"
                     /old_locus_tag="pgaptmp_000880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux transporter transcriptional
                     repressor AcrR"
                     /protein_id="HGU4546868.1"
                     /translation="MARKTKQEAQETRQHILDVALRLFSQQGVSSTSLGEIAKAAGVT
                     RGAIYWHFKDKSDLFSEIWELSESNIGELELEYQAKFPGDPLSVLREILIHVLESTVT
                     EERRRLLMEIIFHKCEFVGEMAVVQQAQRNLCLESYDRIEQTLKHCIEAKMLPADLMT
                     RRAAIIMRGYISGLMENWLFAPQSFDLKKEARDYVAILLEMYLLCPTLRNPATNE"
     gene            5400..8762
                     /gene="mscK"
                     /locus_tag="ACHOSC_RS04405"
                     /old_locus_tag="pgaptmp_000881"
     CDS             5400..8762
                     /gene="mscK"
                     /locus_tag="ACHOSC_RS04405"
                     /old_locus_tag="pgaptmp_000881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive channel MscK"
                     /protein_id="HGU4546869.1"
                     /translation="MTMFQYYKRSRHFVFSAFIAFVFVLLCQNTAFARASSNGDLPTK
                     ADLQAQLDSLNKQKDLSAQDKLVQQDLTDTLATLDKIDRIKEETVQLRQKVAEAPEKM
                     RQATAALTALSDVDNDEETRKILSTLSLRQLETRVAQALDDLQNAQNDLASYNSQLVS
                     LQTQPERVQNAMYNASQQLQQIRSRLDGTDVGETALRPSQKVLMQAQQALLNAEIDQQ
                     RKSLEGNTVLQDTLQKQCDYVTANSARLEHQLQLLQEAVNSKRLTLTEKTAQEAVSPD
                     EAARIQANPLVKQELEINQQLSQRLITATENGNQLMQQNIKVKNWLERALQSERNIKE
                     QIAVLKGSLLLSRILYQQQQTLPSADELENMTNRIADLRLEQFEVNQQRDALFQSDAF
                     VNKLEEGHTNEVNSEVHDALLQVVDMRRELLDQLNKQLGNQLMMAINLQINQQQLMSV
                     SKNLKSILTQQIFWVNSNRPMDWDWIKAFPQSLKDEFKSMKITVNWEKAWPAVFIAFL
                     AGLPLLLIAGLIHWRLGWLKAYQQKLASAVGSLRNDSQLNTPKAILIDLIRALPVCLI
                     ILAVGLILLTMQLNISELLWSFSKKLAIFWLVFGLCWKVLEKNGVAVRHFGMPEQQTS
                     HWRRQIVRISLALLPIHFWSVVAELSPLHLMDDVLGQAMIFFNLLLIAFLVWPMCRES
                     WRDKESHTMRLVTITVLSIIPIALMVLTATGYFYTTLRLSGRWIETVYLVIIWNLLYQ
                     TVLRGLSVAARRIAWRRALARRQNLVKEGAEGAEPPEEPTIALEQVNQQTLRITMLLM
                     FALFGVMFWAIWSDLITVFSYLDSITLWHYNGTEAGAAVVKNVTMGSLLFAIIASMVA
                     WALIRNLPGLLEVLVLSRLNMRQGASYAITTILNYIIIAVGAMTVFGSLGVSWDKLQW
                     LAAALSVGLGFGLQEIFGNFVSGLIILFERPVRIGDTVTIGSFSGTVSKIRIRATTIT
                     DFDRKEVIIPNKAFVTERLINWSLTDTTTRLVIRLGVAYGSDLEKVRKVLLKAATEHP
                     RVMHEPMPEVFFTAFGASTLDHELRLYVRELRDRSRTVDELNRTIDQLCRENDINIAF
                     NQLEVHLHNEKGDEVTEVKRDYKGDDPTPAVG"
     gene            complement(8974..9135)
                     /gene="rsmS"
                     /locus_tag="ACHOSC_RS04410"
                     /old_locus_tag="pgaptmp_000882"
     CDS             complement(8974..9135)
                     /gene="rsmS"
                     /locus_tag="ACHOSC_RS04410"
                     /old_locus_tag="pgaptmp_000882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pleiotropic regulatory protein RsmS"
                     /protein_id="HGU4546870.1"
                     /translation="MSLENAPDDVKLAVDLIVLLEENQIPARTVLRALDIVKRDYEKK
                     LTRDDEAEK"
     gene            complement(9149..9676)
                     /gene="priC"
                     /locus_tag="ACHOSC_RS04415"
                     /old_locus_tag="pgaptmp_000883"
     CDS             complement(9149..9676)
                     /gene="priC"
                     /locus_tag="ACHOSC_RS04415"
                     /old_locus_tag="pgaptmp_000883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal replication protein N''"
                     /protein_id="HGU4546871.1"
                     /translation="MKTALLLEKLEGQLATLRQRCAPVAQFATLSARFDRHLFQTRAT
                     TLQACLDEAGDNLAALRHAVEQQQLPQVAWLAEHLAAQLEAIAREASAWSLREWDSAP
                     PKIARWQRKRIQHQDFERRLREMVAERRARLARVTDLVEQQTLHREVEAYEARLARCR
                     HALEKIENRLARLTR"
     gene            9746..10123
                     /gene="ybaN"
                     /locus_tag="ACHOSC_RS04420"
                     /old_locus_tag="pgaptmp_000884"
     CDS             9746..10123
                     /gene="ybaN"
                     /locus_tag="ACHOSC_RS04420"
                     /old_locus_tag="pgaptmp_000884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF454 family protein"
                     /protein_id="HGU4546872.1"
                     /translation="MQRIILIIIGWLAVVLGTLGVVLPVLPTTPFILLAAWCFARSSP
                     RFHAWLLYRSWFGSYLRFWQKHHAMPRGVKPRAILLILLTFAISLWFVQMPWVRIMLL
                     VILACLLFYMWRIPVIDEKQEKH"
     gene            10276..10827
                     /gene="apt"
                     /locus_tag="ACHOSC_RS04425"
                     /old_locus_tag="pgaptmp_000885"
     CDS             10276..10827
                     /gene="apt"
                     /locus_tag="ACHOSC_RS04425"
                     /old_locus_tag="pgaptmp_000885"
                     /EC_number="2.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005125198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003999 - adenine
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0006168 - adenine salvage [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenine phosphoribosyltransferase"
                     /protein_id="HGU4546873.1"
                     /translation="MTATAQQLEYLKNSIKSIQDYPKPGILFRDVTSLLEDPKAYALS
                     IDLLVERYKNAGITKVVGTEARGFLFGAPVALGLGVGFVPVRKPGKLPRETISETYDL
                     EYGTDQLEIHVDAIKPGDKVLVVDDLLATGGTIEATVKLIRRLGGEVADAAFIINLFD
                     LGGEQRLEKQGITSYSLVPFPGH"
     gene            10956..12887
                     /gene="dnaX"
                     /locus_tag="ACHOSC_RS04430"
                     /old_locus_tag="pgaptmp_000886"
     CDS             10956..12887
                     /gene="dnaX"
                     /locus_tag="ACHOSC_RS04430"
                     /old_locus_tag="pgaptmp_000886"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit gamma/tau"
                     /protein_id="HGU4546874.1"
                     /translation="MSYQVLARKWRPQTFADVVGQEHVLTALANGLSLGRIHHAYLFS
                     GTRGVGKTSIARLLAKGLNCETGITATPCGVCDNCREIEQGRFVDLIEIDAASRTKVE
                     DTRDLLDNVQYAPARGRFKVYLIDEVHMLSRHSFNALLKTLEEPPEHVKFLLATTDPQ
                     KLPVTILSRCLQFHLKALDVEQIRHQLEHILNEEHIAHEPRALQLLARAAEGSLRDAL
                     SLTDQAIASGDGQVSTQAVSAMLGTLDDDQALSLVEAMVEANGERVMALINEAAARGI
                     EWEALLVEMLGLLHRIAMVQLSPAALGNDMAAIELRMRELARTIPPTDIQLYYQTLLI
                     GRKELPYAPDRRMGVEMTLLRALAFHPRMPLPEPEVPRQSFAPVAPTAVMTPTQVPPQ
                     PQSAPQQAPTVPLPETTSQVLAARQQLQRVQGATKAKKSEPAAATRARPVNNAALERL
                     ASVTDRVQARPVPSALEKAPAKKEAYRWKATTPVMQQKEVVATPKALKKALEHEKTPE
                     LAAKLAAEAIERDAWAAQVSQLSLPKLVEQVALNAWKEESDNAVCLHLRSSQRHLNNR
                     GAQQKLAEALSTLKGSTVELTIVEDDNPAVRTPLEWRQAIYEEKLAQARESIIADNNI
                     QTLRRFFDAELDEESIRPI"
     misc_feature    12220..12284
                     /gene="dnaX"
                     /locus_tag="ACHOSC_RS04430"
                     /old_locus_tag="pgaptmp_000886"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00382"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="DnaX frameshifting element; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /db_xref="RFAM:RF00382"
     gene            12940..13269
                     /gene="ybaB"
                     /locus_tag="ACHOSC_RS04435"
                     /old_locus_tag="pgaptmp_000887"
     CDS             12940..13269
                     /gene="ybaB"
                     /locus_tag="ACHOSC_RS04435"
                     /old_locus_tag="pgaptmp_000887"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001364632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YbaB/EbfC family nucleoid-associated protein"
                     /protein_id="HGU4546875.1"
                     /translation="MFGKGGLGNLMKQAQQMQEKMQKMQEEIAQLEVTGESGAGLVKV
                     TINGAHNCRRVEIDPSLLEDDKEMLEDLVAAAFNDAARRIEETQKEKMASVSSGMQLP
                     PGFKMPF"
     gene            13269..13874
                     /gene="recR"
                     /locus_tag="ACHOSC_RS04440"
                     /old_locus_tag="pgaptmp_000888"
     CDS             13269..13874
                     /gene="recR"
                     /locus_tag="ACHOSC_RS04440"
                     /old_locus_tag="pgaptmp_000888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombination mediator RecR"
                     /protein_id="HGU4546876.1"
                     /translation="MQTSPLLTQLMEALRCLPGVGPKSAQRMAFTLLQRDRSGGMRLA
                     QALTRAMSEIGHCADCRTFTEQEVCNICSNPRRQENGQICVVESPADIYAIEQTGQFS
                     GRYFVLMGHLSPLDGIGPDDIGLDRLEQRLAEEKITEVILATNPTVEGEATANYIAEL
                     CAQYDVEASRIAHGVPVGGELEMVDGTTLSHSLAGRHKIRF"
     gene            13984..15858
                     /gene="htpG"
                     /locus_tag="ACHOSC_RS04445"
                     /old_locus_tag="pgaptmp_000889"
     CDS             13984..15858
                     /gene="htpG"
                     /locus_tag="ACHOSC_RS04445"
                     /old_locus_tag="pgaptmp_000889"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003903109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone HtpG"
                     /protein_id="HGU4546877.1"
                     /translation="MKGQETRGFQSEVKQLLHLMIHSLYSNKEIFLRELISNASDAAD
                     KLRFRALSNPDLYEGDGELRVRVSFDKDKRTLTISDNGVGMTRDEVIDHLGTIAKSGT
                     KSFLESLGSDQAKDSQLIGQFGVGFYSAFIVADKVTVRTRAAGEKPENGVFWESAGEG
                     EYTVADITKEDRGTEITLHLREGEDEFLDDWRVRSIISKYSDHIALPVEIEKREEKDG
                     ETVISWEKINKAQALWTRNKSEITDEEYKEFYKHIAHDFNDPLTWSHNRVEGKQEYTS
                     LLYIPSQAPWDMWNRDHKHGLKLYVQRVFIMDDAEQFMPNYLRFVRGLIDSSDLPLNV
                     SREILQDSTVTRNLRNALTKRVLQMLEKLAKDDADKYQTFWQQFGLVLKEGPAEDFAN
                     QEAIAKLLRFASTYTDSSAQTVSLEDYVSRMKEGQEKIYYITADSYAAAKSSPHLELL
                     RKKGIEVLLLSDRIDEWMMNYLTEFDGKPFQSVSKVDESLEKLADEVDESAKEAEKAL
                     TPFIDRVKALLGERVKDVRLTHRLTDTPAIVSTDADEMSTQMAKLFAAAGQKVPEVKY
                     IFELNPDHVLVKRAADTEDEAKFSEWVELLLDQALLAERGTLEDPNLFIRRMNQLLVS
                     "
     gene            16039..16683
                     /gene="adk"
                     /locus_tag="ACHOSC_RS04450"
                     /old_locus_tag="pgaptmp_000890"
     CDS             16039..16683
                     /gene="adk"
                     /locus_tag="ACHOSC_RS04450"
                     /old_locus_tag="pgaptmp_000890"
                     /EC_number="2.7.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007706188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004017 - adenylate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006139 - nucleobase-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate kinase"
                     /protein_id="HGU4546878.1"
                     /translation="MRIILLGAPGAGKGTQAQFIMEKYGIPQISTGDMLRAAVKSGSE
                     LGKQAKDIMDAGKLVTDELVIALVKERIAQEDCRNGFLLDGFPRTIPQADAMKEAGIN
                     VDYVLEFDVPDELIVDRIVGRRVHAPSGRVYHVKFNPPKVEGKDDVTGEELTTRKDDQ
                     EETVRKRLVEYHQMTAPLIGYYSKEAEAGNTKYAKVDGTKPVAEVRADLEKILG"
     gene            16919..17881
                     /gene="hemH"
                     /locus_tag="ACHOSC_RS04455"
                     /old_locus_tag="pgaptmp_000891"
     CDS             16919..17881
                     /gene="hemH"
                     /locus_tag="ACHOSC_RS04455"
                     /old_locus_tag="pgaptmp_000891"
                     /EC_number="4.98.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004325 - ferrochelatase activity
                     [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferrochelatase"
                     /protein_id="HGU4546879.1"
                     /translation="MRQTKTGILLANLGTPDAPTPEAVKRYLKQFLSDRRVVDTSRLL
                     WWPLLRGVILPLRSPRVAKMYASVWMEDGSPLMVYSRQQQQALAQRLPDTPVALGMSY
                     GSPSLESAVDELLAEHVDHIVVLPLYPQFSCSTVGAVWDELARILARKRSIPGISFIR
                     DYADNHDYINALANSVRASFAKHGEPDLLLLSYHGIPQRYADEGDDYPQRCRTTTREL
                     ASALGMAPEKVMMTFQSRFGREPWLMPYTDETLKMLGEKGVGHIQVMCPGFAADCLET
                     LEEIAEQNREVFLGAGGKKYEYIPALNATPEHIEMMANLVAAYR"
     gene            complement(17878..18837)
                     /gene="aes"
                     /locus_tag="ACHOSC_RS04460"
                     /old_locus_tag="pgaptmp_000892"
     CDS             complement(17878..18837)
                     /gene="aes"
                     /locus_tag="ACHOSC_RS04460"
                     /old_locus_tag="pgaptmp_000892"
                     /EC_number="3.1.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl esterase"
                     /protein_id="HGU4546880.1"
                     /translation="MKPENKLPVLDLISAEMKTVVNTLQPDLPSWPATGTIAEQRQYY
                     TLERRFWNAGAPEMATRAYMVPTKYGQVETRLFCPQPDSPATLFYLHGGGFILGNLDT
                     HDRIMRLLASYSQCTVIGIDYPLSPEARFPQAIEEIVAACCYFHQQAEDYQINMSRIG
                     FAGDSAGAMLALASALWLRDKQIDCGKIAGVLLWYGLYGLRDSVTRRLLGGVWDGLTQ
                     QDLQMYEEAYLSNDADRESPYYCLFNNDLTREVPPCFIAGAEFDPLLDDSRLLYQTLA
                     AHQQPCEFKLYPGTLHAFLHYSRMMKTADEALRDGAQFFTAQL"
     gene            18989..20293
                     /gene="gsk"
                     /locus_tag="ACHOSC_RS04465"
                     /old_locus_tag="pgaptmp_000893"
     CDS             18989..20293
                     /gene="gsk"
                     /locus_tag="ACHOSC_RS04465"
                     /old_locus_tag="pgaptmp_000893"
                     /EC_number="2.7.1.73"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inosine/guanosine kinase"
                     /protein_id="HGU4546881.1"
                     /translation="MKFPGKRKSKHYFPVNARDPLLQQFQPENETSAAWVVGIDQTLV
                     DIEAKVDDEFIERYGLSAGHSLVIEDDVAEALYQELKQKNLITHQFAGGTIGNTMHNY
                     SVLADDRSVLLGVMCSNIEIGSYAYRYLCNTSSRTDLNYLQGVDGPIGRCFTLIGESG
                     ERTFAISPGHMNQLRAESIPEDVIAGASALVLTSYLVRCKPGEPMPEATMKAIEYAKK
                     YNVPVVLTLGTKFVIAENPQWWQQFLKDHVSILAMNEDEAEALTGESDPLLASDKALD
                     WVDLVLCTAGPIGLYMAGFTEDEAKRKTQHPLLPGAIAEFNQYEFSRAMRHKDCQNPL
                     RVYSHIAPYMGGPEKIMNTNGAGDGALAALLHDITANSYHRSNVPNSSKHKFTWLTYS
                     SLAQVCKYANRVSYQVLNQHSPRLTRGLPEREDSLEESYWDR"
     gene            complement(20426..22102)
                     /gene="ybaL"
                     /locus_tag="ACHOSC_RS04470"
                     /old_locus_tag="pgaptmp_000894"
     CDS             complement(20426..22102)
                     /gene="ybaL"
                     /locus_tag="ACHOSC_RS04470"
                     /old_locus_tag="pgaptmp_000894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0051139 - metal cation:proton antiporter
                     activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YbaL family putative K(+) efflux transporter"
                     /protein_id="HGU4546882.1"
                     /translation="MHHATPLITTIVGGLVLAFILGMLANKLRISPLVGYLLAGVLAG
                     PFTPGFVADTKLAPELAELGVILLMFGVGLHFSLKDLMAVKSIAIPGAIAQIAVATLL
                     GMALSAVLGWSLMTGIVFGLCLSTASTVVLLRALEERQLIDSQRGQIAIGWLIVEDLV
                     MVLTLVLLPAVAGMMEQGDVGFATLAVDMGITIGKVIAFIAIMMLVGRRLVPWIMARS
                     AATGSRELFTLSVLALALGIAFGAVELFDVSFALGAFFAGMVLNESELSHRAAHDTLP
                     LRDAFAVLFFVSVGMLFDPLILIQQPLAVLATLAIILFGKSLAAFFLVRLFGHSQRTA
                     LTIAASLAQIGEFAFILAGLGMALNLLPQAGQNLVLAGAILSIMLNPVLFALLEKYLA
                     KTETLEEQTLEEAIEEEKQIPVDICNHALLVGYGRVGSLLGEKLLASDIPLVVIETSR
                     TRVDELRERGVRAVLGNAANEEIMQLAHLECAKWLILTIPNGYEAGEIVASARAKNPD
                     IEIIARAHYDDEVAYITERGANQVVMGEREIARTMLELLETPPAGEVVTG"
     gene            complement(22340..23560)
                     /gene="fsr"
                     /locus_tag="ACHOSC_RS04475"
                     /old_locus_tag="pgaptmp_000895"
     CDS             complement(22340..23560)
                     /gene="fsr"
                     /locus_tag="ACHOSC_RS04475"
                     /old_locus_tag="pgaptmp_000895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fosmidomycin MFS transporter"
                     /protein_id="HGU4546883.1"
                     /translation="MAMSEQPQPVAGAAASTTKARTSFGILGAISLSHLLNDMIQSLI
                     LAIYPLLQSEFSLTFMQIGMITLTFQLASSLLQPVVGYWTDKYPMPWSLPIGMCFTLS
                     GLVLLALAGSFGAVLLAAALVGTGSSVFHPESSRVARMASGGRHGLAQSIFQVGGNFG
                     SSLGPLLAAVIIAPYGKGNVAWFVLAALLAIVVLAQISRWYSAQHRMNKGKPKATIIN
                     PLPRNKVVLAVSILLILIFSKYFYMASISSYYTFYLMQKFGLSIQNAQLHLFAFLFAV
                     AAGTVIGGPVGDKIGRKYVIWGSILGVAPFTLILPYASLHWTGVLTVIIGFILASAFS
                     AILVYAQELLPGRIGMVSGLFFGFAFGMGGLGAAVLGLIADHTSIELVYKICAFLPLL
                     GMLTIFLPDNRHKD"
     gene            23778..25430
                     /gene="ushA"
                     /locus_tag="ACHOSC_RS04480"
                     /old_locus_tag="pgaptmp_000896"
     CDS             23778..25430
                     /gene="ushA"
                     /locus_tag="ACHOSC_RS04480"
                     /old_locus_tag="pgaptmp_000896"
                     /EC_number="3.1.3.5"
                     /EC_number="3.6.1.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-sugar
                     hydrolase/5'-nucleotidase"
                     /protein_id="HGU4546884.1"
                     /translation="MKLLQRGVALALLTTFTLASETALAYEQDKTYKITVLHTNDHHG
                     HFWRNEYGEYGLAAQKTLVDGIRKEVAAEGGSVLLLSGGDINTGVPESDLQDAEPDFR
                     GMNLVGYDAMAIGNHEFDNPLTVLRQQEKWAKFPLLSANIYQKSTGERLFKPWALFKR
                     QDLKIAVIGLTTDDTAKIGNPEYFTDVEFRKPADEAKLVIQELQQTEKPDIIIAATHM
                     GHYDNGEHGSNAPGDVEMARALPAGSLAMIVGGHSQDPVCMAAENKKQVDYVPGTPCK
                     PDQQNGIWIVQAHEWGKYVGRADFEFRNGKMKMVNYQLIPVNLKKKVTWEDGKSERVL
                     YTPEIAENQQMISLLSPFQNKGKAQLEVKIGETNGRLEGDRDKVRFVQTNMGRLILAA
                     QMDRTGADFAVMSGGGIRDSIEAGDISYKNVLKVQPFGNVVVYADMTGKEVIDYLTAV
                     AQMKPDSGAYPQFANVSFVAKDGRLNDLKIKGEPVDPAKTYRMATLNFNATGGDGYPR
                     LDNKPGYVNTGFIDAEVLKAYIQKSSPLDVSVYEPKGEVSWQ"
     gene            complement(25467..25946)
                     /gene="ybaK"
                     /locus_tag="ACHOSC_RS04485"
                     /old_locus_tag="pgaptmp_000897"
     CDS             complement(25467..25946)
                     /gene="ybaK"
                     /locus_tag="ACHOSC_RS04485"
                     /old_locus_tag="pgaptmp_000897"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006818871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0002161 - aminoacyl-tRNA editing activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Cys-tRNA(Pro)/Cys-tRNA(Cys) deacylase YbaK"
                     /protein_id="HGU4546885.1"
                     /translation="MTPAVKLLEKNKISFQIHTYEHDPAETNFGDEVVKKLGLNPDQV
                     YKTLLVAVNGDMKHLAVAVTPVAGQLDLKKVAKALGAKKVEMADPMVAQRSTGYLVGG
                     ISPLGQKKRLPTIIDAPAQEFATIYVSGGKRGLDIELAAGDLAKILDAKFADIARRD"
     gene            complement(26150..26944)
                     /gene="ybaP"
                     /locus_tag="ACHOSC_RS04490"
                     /old_locus_tag="pgaptmp_000898"
     CDS             complement(26150..26944)
                     /gene="ybaP"
                     /locus_tag="ACHOSC_RS04490"
                     /old_locus_tag="pgaptmp_000898"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TraB/GumN family protein"
                     /protein_id="HGU4546886.1"
                     /translation="MDLLYRVKTLWAALRGNHYTWPAIDITLPGNRHFHLIGSIHMGS
                     HDMAPLPTRLLKKLKNADALIVEADVSTSDTPFANLPACEALEERISEEQLQNLQHIS
                     QEMGISPSLFSTQPLWQIAMVLQATQAQKLGLRAEYGIDYQLLQAAKQQHKPVIELEG
                     AENQIAMLLQLPDKGLALLDDTLTHWHTNARLLQQMMSWWLNAPPQNNEITLPNTFSQ
                     SLYDVLMHQRNLAWRDKLRAMPPGRYVVAVGALHLYGEGNLPQMLR"
     gene            27082..27423
                     /gene="ybaQ"
                     /locus_tag="ACHOSC_RS04495"
                     /old_locus_tag="pgaptmp_000899"
     CDS             27082..27423
                     /gene="ybaQ"
                     /locus_tag="ACHOSC_RS04495"
                     /old_locus_tag="pgaptmp_000899"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415016.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HigA family addiction module antitoxin"
                     /protein_id="HGU4546887.1"
                     /translation="MKQATRKPTTPGDILLYEYLVPLDLKINELAELLHVHRNSVSAL
                     INNNRKLTTEMAFRLAKVFDTTVDFWLNLQAAVDLWEVENNMRTQEELGRIETVAEYL
                     ARREERAKKVA"
     gene            complement(27537..30041)
                     /gene="copA"
                     /locus_tag="ACHOSC_RS04500"
                     /old_locus_tag="pgaptmp_000900"
     CDS             complement(27537..30041)
                     /gene="copA"
                     /locus_tag="ACHOSC_RS04500"
                     /old_locus_tag="pgaptmp_000900"
                     /EC_number="7.2.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019829 - ATPase-coupled monoatomic cation
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="copper-exporting P-type ATPase CopA"
                     /protein_id="HGU4546888.1"
                     /translation="MSQIIDLTLDGLSCGHCVKRVKESLEQRPDVEQADVSITEAHVT
                     GTASAEQLIETIKQAGYDASVSHPKAKPLAESSIPSEALTAVSEALPAATADDDDSQQ
                     LLLSGMSCASCVTRVQNALQSVPGVTQARVNLAERTALVMGSASPQDLVQAVEKAGYG
                     AEAIEDDAKRRERQQETAVATMKRFRWQAIVALAVGIPVMVWGMIGDNMMVTADNRSL
                     WLVIGLITLAVMVFAGGHFYRSAWKSLLNGAATMDTLVALGTGVAWLYSMSVNLWPQW
                     FPMEARHLYYEASAMIIGLINLGHMLEARARQRSSKALEKLLDLTPPTARLVTDEGEK
                     SVPLAEVQPGMLLRLTTGDRVPVDGEITQGEAWLDEAMLTGEPIPQQKGEGESVHAGT
                     VVQDGSVLFRASAVGSHTTLSRIIRMVRQAQSSKPEIGQLADKISAVFVPVVVVIALV
                     SAAIWYFFGPAPQIVYTLVIATTVLIIACPCALGLATPMSIISGVGRAAEFGVLVRDA
                     DALQRASTLDTVVFDKTGTLTEGKPQVVAVKTFADVDEAQALRLAAALEQGSSHPLAR
                     AILDKAGDMQLPQVNGFRTLRGLGVSGEAEGHALLLGNQALLNEQQVGTKAIEAEITA
                     QASQGATPVLLAVDGKAVALLAVRDPLRSDSVAALQRLHKAGYRLVMLTGDNPTTANA
                     IAKEAGIDEVIAGVLPDGKAEAIKRLQSEGRQVAMVGDGINDAPALAQADVGIAMGGG
                     SDVAIETAAITLMRHSLMGVADALAISRATLRNMKQNLLGAFIYNSIGIPVAAGILWP
                     FTGTLLNPVVAGAAMALSSITVVSNANRLLRFKPKE"
     gene            30303..31235
                     /gene="glsA"
                     /locus_tag="ACHOSC_RS04505"
                     /old_locus_tag="pgaptmp_000901"
     CDS             30303..31235
                     /gene="glsA"
                     /locus_tag="ACHOSC_RS04505"
                     /old_locus_tag="pgaptmp_000901"
                     /EC_number="3.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004359 - glutaminase activity [Evidence
                     IEA];
                     GO_process: GO:0006541 - glutamine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutaminase A"
                     /protein_id="HGU4546889.1"
                     /translation="MLDANKLQQAVDQAYTQFHSLNGGQNADYIPFLANVPSQLAAVA
                     IVTCDGNIYRAGDSDYRFALESISKVCTLALALEDVGPQAVQDKIGADPTGLPFNSVI
                     ALELHGGKPLSPLVNAGAIATTSLINAENIEQRWQRILHIQQQLAGEQVALSDEVNQS
                     EQTTNFHNRAIAWLLYSAGYLYCDAMEACDVYTRQCSTLINTVELATLGATLAAGGVN
                     PLTHKRVLQADNVPYILAEMMMEGLYGRSGDWAYRVGLPGKSGVGGGILAVVPGVMGI
                     AAFSPPLDEEGNSVRGQKMVASVAKQLGYNVFKG"
     gene            31238..>31308
                     /locus_tag="ACHOSC_RS04510"
                     /old_locus_tag="pgaptmp_000902"
     CDS             31238..>31308
                     /locus_tag="ACHOSC_RS04510"
                     /old_locus_tag="pgaptmp_000902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4546890.1"
                     /translation="MMNTEGNNGNKPLGLWNVVSIGIG"
BASE COUNT         7658 a         8308 c         8202 g         7140 t
ORIGIN      
        1 cgtggttaat actggttttc gtatgagatc ctgagttggt ggttcaatta ctccttaatg
       61 ttcgtaggtt atgcataaaa aaggccgctt gcgcggcctt agtgattaca cgttgtatca
      121 atgatgatcg acagtatggc tgtgctcgat atcttcattc ttgcggctaa agcggcggcg
      181 aaccaccaca aagaataccg gaacgaagaa gattgccagt accgttgcgg tcaccatccc
      241 gcccattaca ccggtaccta ctgcgttctg cgcgccggaa ccagcaccag tactgataac
      301 cagcggcata acgccgagga taaacgccag cgaggtcatc aggatcggac gtaaacgcat
      361 ccgcactgca tcaagcgtcg cttcaatcag acctttacct tctttatcca tcaagtcttt
      421 ggcgaattcg acgataagta tcgcgttctt cgccgacaac ccaatggttg tgagcaggcc
      481 tacctggaag taaacgtcat tggtcaggcc acggaaggtg gcagccagca acgcaccgat
      541 aacccccagc ggaacgacca gcataacgga gaacggaatc gaccagctct cgtacagcgc
      601 cgccagacac aggaatacga caatcaacga aatcgcgtac agtgaaggtg cctggttgcc
      661 ggagaggcgt tcctgatagg acatccccgt ccagtcatag ccaacaccgg taggcagttt
      721 gctcgccagt tgttccatca gctccattgc ttcaccggta cttttacccg gtgccgcctg
      781 gcctaagatt tccatggatg gcaggccatt gtaacgttcc agacgcggcg aaccgtactc
      841 ccaacgagaa gaggagaatg ccgagaacgg caccatctga ccatcagcag cacgaacata
      901 ccagtcgccg atatcatccg gcagcatacg gtatttcgct tctgacatga cgtaaacttt
      961 cttcacacga ccgcggtcga taaagtcgtt cacatagcta ccgccccatg cagcgcccag
     1021 agtggtgtta atgtcattga tagaaacacc cagcgcctgc gctttttccc ggtcgatatc
     1081 aatcttaaac tgcggggtat cttccagacc gtttggacgt acgctggtca acatatcagg
     1141 gtgcttcgct gcttctgcaa gcaactggtt acgcgcctga gtcagttttt cgtgaccaag
     1201 gccagcctgg tcaatcagct caaagtcaaa gccggttgca gtacccagtt ccacgattgc
     1261 gggcaggtta aaggcgaaaa ccatcgcatc tttgatttgc gagaaagcgc gtgttgcacg
     1321 catggtaatc gcttcaactt tgttttcttc gcccggacga tcggcccagt ccttcaagga
     1381 aacgaacgca ataccggtat tctgaccacg tcccgcaaag ccgaagccgt taacggcgaa
     1441 caccgactca acgttgttct tttctttggt cagatagtaa tgcgttacct cattgagcac
     1501 tttctgcgta cgttcctgcg ttgcacctgc tggcagctga accatggtca taaatacgcc
     1561 ctggtcctca tctggcaaga aggagcttgg cagacgcacg aacagatagg ccatgccgac
     1621 cacgatgatc agatacagca ccaggtaacg ccccgtactg cgcagaatac cgcctacgct
     1681 gtcggtgtag tggtgcgtgc tcttctcgaa catgcggtta aaccagccga agaagccttt
     1741 tttaccttcc ccgtgatcgc ctttggcaat cggtttcagc atggtggcac aaagagccgg
     1801 agtcaggatc aacgctacca gtaccgacag cgccattgct gaaacaatgg taatagagaa
     1861 ctgacgatag atagcaccag tagaaccgcc aaagaaggcc atcggtacga ataccgccga
     1921 cagtaccatc gcgataccga ccagagcgcc ctgaatctgc cccatcgact tacgggtagc
     1981 ttcttttggc ggcaaacctt cttccgccat aacacgctcc acgttttcta ccacaacgat
     2041 agcgtcatcc accaacaagc cgatggcgag caccatcccg aacattgtta gcgtgtttat
     2101 cgagaagcca aaggcggcaa ggacggcaaa ggtcccgagc aataccaccg gtacggcaat
     2161 ggtcggaatc aacgtcgcgc ggaagttctg caggaacaga tacataacca ggaacacgag
     2221 gatgatcgct tcgaccagcg ttttaaccac ttcgtgaata gagattttca cgaacggcgt
     2281 ggtgtcgtac gggtaaacaa ttttcagacc cgacgggaag aacggttcca tcttcgccag
     2341 ttcagcacgg attgccgcag cggtatccag cgcgtttgca ccggtcgcca gcttgatccc
     2401 cagaccggaa gccggttggc cgttaaactc tgcgatgatg tcgtagttct caccacccag
     2461 ctcaatcttc gccacatcac gcagcagtac gcgggaacca tcctgattca ctttcagcag
     2521 gattttgccg aactcttcag tagaggtcag acgcgtctga gcaataatag aggcgttaag
     2581 ctgttggcct ttcaccggcg gcgtaccacc gagctgaccc gccgcaacct gggcgttctg
     2641 cgctttgatg gcggtaatga catcaaccgg cgttagctgg aatttgttca gctcattcgg
     2701 gttcatccag atacgcatcg cgtactgtga accgaacaac tgaacatcac ccacgcccga
     2761 cgtacggctg atggcatctt tcatattcgc cgctacgtag tcagagatat cctcctgcgt
     2821 catggtgcca tcggtgttga taacgccgac aaccatcagg aagctgctgg atgatttctc
     2881 aacgctcacc ccttgctgct gaacttcttg cggcagcaac ggcatcgcca gctgcagttt
     2941 gttctgcacc tgaacctgcg cgatatccgc atcagtacca gactcaaagg tcagggtgat
     3001 ctgcacggta cccgtggagt cactgttaga ggacatgtac atcaggttat cgataccgtt
     3061 catattctgt tcgataacct gtgtcaccgt gtcctgcact gttttcgcat cagcgccggg
     3121 gtaggaggcg gagatcgtta ctgccggcgg tgcaatcgta ggatattgcg ccaccggcag
     3181 tttgaggatc gccagccccc ctgccaacat gatgataatg gcgatcaccc acgcaaaaat
     3241 cgggcgatcg ataaagaaat taggcatgtc ttaacggctc ctgtttaagt taagacttgg
     3301 actgttcagg ctgagcaccg cttgcggctt gctggttatt atcagcggta acttcttgtg
     3361 cttttacctg gacaccagga cgcactttct gcagcccact tattactacg cgatcgcctg
     3421 ctttcagacc ttctgtcacc agccacttat cgccaatagc ctggcttgca acgatcggac
     3481 gggtttccac tttgtcatcc gcgccaacta ccagtacggt ggcatcacca cgcggcgtac
     3541 gggttacgcc ctgttgcggg actaaaatag cgtttggatt aagcccttct tccagacgtg
     3601 cgcgcacgaa catacccggc agcagagtgt gatccgggtt cgggaagata gcgcgtaggg
     3661 tgatagaccc agtggtctga tcaacggtaa cgtcagagaa ttccagcgta ccgtcctgcg
     3721 ggaacttaat gccgtcactg gtgatcagcg acactttggc tttgccgttc tcttgtttca
     3781 gcgtgccatt cgccagttcc tgtttcaggc gcaggaagtc gttgctggac tgggtcacat
     3841 caacgtagat cggatcaagt tgctgcacgg ttgccagcgc agtcgcctga ccgttctgta
     3901 ccaatgcgcc ttccgtcacg ttcgacttac caatgcgacc gctaatcgga gaggtgactt
     3961 tggtgtaagc cagattgatc cgcgcagttt caacggcagc tttcgccgca gttaccgcag
     4021 cattcgcctg ttgcgcatca gccagagcct gatcgtactc ttgcttactg atgtactgag
     4081 taccgagcag tttctgataa cgattcaccg tcaattgcgc gatattggct gcagcctggg
     4141 ctttcgccag atcacctttc gcactgtcgt atgtcgcctg ataggtcgca ggatcaatct
     4201 gatagagaga gacaccagct tcgatgtcgc taccttcttt gaaattacgc ttcaggataa
     4261 tcccgctaac ttgaggacga acttctgcga tccggtaggc actggtgcga cccggaagct
     4321 cggttgtgat ctgcagaggt tcagttttga ctgttactac gccaacggcg ggcatctgct
     4381 ggccaccttg ttgggcctgt ttgtcgtcac atcctgttag ggctaagctg cctgagagca
     4441 tcagaacgac cgccagaggc gtaaaccctc tgtttttgtt catatgtaaa cctcgagtgt
     4501 ccgatttcaa attggtcaat ggtcaaaagt taataaaccc attgctgcgt ttatattatc
     4561 gtcgtgctat ggtacataca ttcacaaatg tatgtaaatc taacgcctgt aaattcacga
     4621 acatatggca cgaaaaacca aacaagaagc gcaagaaacg cgccaacaca tcctcgatgt
     4681 ggctctacgt cttttctcac agcagggggt atcatccacc tcgctgggtg agattgcaaa
     4741 agcagctggc gttacgcgcg gtgcaatcta ctggcatttt aaagacaagt cggatttgtt
     4801 cagtgagatc tgggaactgt cagaatccaa tattggtgaa ctagagcttg agtatcaggc
     4861 aaaattccct ggcgatccac tctcagtatt aagagagata ttaattcatg ttcttgaatc
     4921 cacggtgaca gaagaacggc gtcgattatt gatggagatt atattccaca aatgcgaatt
     4981 tgtcggagaa atggctgttg tgcaacaggc acaacgtaat ctctgtctgg aaagttatga
     5041 ccgtatagaa caaacgttaa aacattgtat tgaagcgaaa atgttgcctg cggatttaat
     5101 gacgcgtcgc gcagcaatta ttatgcgcgg ctatatttcc ggcctgatgg aaaactggct
     5161 ctttgccccg caatcttttg atcttaaaaa agaagcccgc gattacgttg ccatcttact
     5221 ggagatgtat ctcctgtgcc ccacgcttcg taatcctgcc actaacgaat aaccctgaat
     5281 ctgactccag gatttttcct ggacattttc gtcgttgcta ttctggttca ctgcgtcgtg
     5341 atattcttgc ggtttgactt tttcaggtcg ttcttcaggt tcagaaacct tcattcatca
     5401 tgactatgtt ccagtattac aaacgatcac ggcattttgt tttttcagca tttattgctt
     5461 ttgtttttgt cttgttatgc cagaacacgg cgtttgcgcg ggcgtcatcg aatggtgatc
     5521 tgccgacaaa agcggacctg caggcgcaac ttgactcact aaataaacaa aaagatcttt
     5581 ctgctcagga caaactggtg cagcaggatc tgacagatac attagccacc ctcgataaaa
     5641 tcgatcgcat aaaagaagag acagttcagc tacgacaaaa agtcgctgaa gcgccggaaa
     5701 aaatgcgcca ggcgaccgcg gcgttaacag cacttagcga tgtcgataac gacgaagaaa
     5761 cgcgcaaaat tctgagtacg ctttcgttgc gccagctgga aactcgcgtt gcccaggcgc
     5821 tggacgattt gcaaaacgca caaaacgatc tggcgtctta taacagccag ctggtttcgt
     5881 tacagacgca gcccgaacgc gtgcaaaatg cgatgtataa cgcttcgcag cagctgcaac
     5941 aaattcgcag tcgtctggat gggactgatg tcggcgagac tgccttacgt cccagccaga
     6001 aagtgttaat gcaggctcag caggcgttgc tgaatgcgga gattgaccag cagcgtaaaa
     6061 gcctggaagg gaacaccgtc ttgcaggata ccttgcaaaa gcaatgtgat tacgtgacgg
     6121 cgaacagcgc ccgtctggag caccagttgc agctgttgca agaagcggta aacagcaagc
     6181 gcctgacttt aactgaaaaa acggcgcagg aagccgtctc cccggatgaa gccgcgcgta
     6241 ttcaggctaa tccgctggtg aaacaggaac tggaaattaa ccagcagtta agtcagcgtc
     6301 tgattaccgc gactgaaaac ggtaatcagt tgatgcagca aaacattaaa gtcaaaaact
     6361 ggctggagcg ggcgctgcaa tcggaacgca atattaaaga gcagattgcc gtcctgaagg
     6421 gcagcctgct gttgtctcgt atcctttacc agcaacaaca aacgctgccc tcggcggatg
     6481 aactggaaaa catgaccaac cgcatcgcgg atttgcgtct cgaacagttt gaagttaacc
     6541 agcagcgtga tgcactcttc cagagcgatg cgttcgtcaa caaactggaa gaaggtcaca
     6601 ccaacgaagt caacagcgaa gttcacgatg cgttattgca agtggttgat atgcgtcgcg
     6661 aattgctgga tcaactcaac aaacagttgg gtaaccagct gatgatggcc attaacctgc
     6721 aaatcaacca gcagcagtta atgagcgtat cgaaaaacct gaaatccatc ctgactcagc
     6781 aaatcttttg ggtgaacagt aaccgtccaa tggactggga ctggatcaaa gcgttcccgc
     6841 aaagcctgaa agatgaattt aagtcgatga aaatcacggt gaactgggaa aaagcctggc
     6901 ctgccgtttt tatcgctttc ctcgccggtt tgccgctgct gttgattgcc gggctgatcc
     6961 actggcgtct gggctggctg aaagcgtatc aacagaaact ggcttccgct gtgggttccc
     7021 tgcgtaacga tagccagctc aacacaccaa aagcgatcct tatcgacctg atccgcgcgc
     7081 tgccggtgtg cctgattatt ctcgcggttg gcctgattct gttgaccatg cagctcaaca
     7141 tcagcgaact gctatggtcg ttcagtaaaa aactggcgat tttctggctg gtgtttggcc
     7201 tgtgctggaa ggtactggag aaaaatggcg ttgccgtgcg tcacttcggc atgccggagc
     7261 agcagaccag ccactggcgt cggcaaattg tccgcatcag tctcgcgttg ctgcctatcc
     7321 atttctggtc tgtggtggca gaactttccc cgctgcatct gatggatgat gtgctggggc
     7381 aggcgatgat tttcttcaac ctgctgctga ttgccttcct ggtatggcca atgtgccgcg
     7441 aaagctggcg tgataaagag tcgcacacca tgcgactggt caccattacc gtgctgtcga
     7501 ttatcccgat tgcattgatg gtgctgactg ccacgggtta tttctacacc acgctgcgcc
     7561 tctccgggcg ttggattgaa accgtttatc tggtgatcat ctggaacttg ctctaccaga
     7621 cggtattgcg cggcttaagc gtagcggcgc ggcgtatcgc ctggcgtcgt gcgttggcgc
     7681 gtcggcagaa tctggtgaaa gagggcgcag aaggtgccga accgccggaa gaacccacca
     7741 ttgcactgga acaggttaac cagcagacgc tgcgtattac catgttgctg atgtttgcgc
     7801 tgttcggtgt catgttctgg gcaatttggt ccgatttgat caccgtgttc agctatctcg
     7861 acagcatcac gctctggcat tacaacggca ctgaagctgg cgctgcggtg gtgaaaaacg
     7921 tcaccatggg cagtctgttg tttgcgatta tcgcctcaat ggtggcctgg gcgttgattc
     7981 gcaacctgcc tggtttactg gaagtgctgg tgctctcgcg actgaatatg cgtcagggcg
     8041 cgtcgtatgc cattactacc atccttaact acatcattat tgccgttggt gcgatgacgg
     8101 tgttcggatc gctgggcgtc tcttgggata aactccagtg gctggccgca gcattatccg
     8161 taggtcttgg ttttggttta caagaaattt tcggtaactt cgtctccggt ttgatcattc
     8221 tgttcgaacg tccggtgcgt attggcgata cggtaaccat tggtagcttc tcggggacgg
     8281 taagtaagat ccgtattcgt gcgacaacga ttaccgattt cgatcgcaaa gaagtgatca
     8341 tcccgaacaa agcgtttgtt accgagcgtc tgatcaactg gtcgttgact gacactacta
     8401 cgcgtctggt gatccgtctc ggcgtggcct atggctccga tctggaaaaa gtgcgtaaag
     8461 tgttactgaa ggcggcgact gagcacccaa gggtgatgca cgaaccaatg ccggaagtct
     8521 tctttacggc gtttggtgcc agcacgttgg atcatgagct gcgtctgtat gtgcgtgaac
     8581 tgcgtgaccg tagtcgtact gtcgatgagc tgaaccgtac tatcgatcag ctgtgccgtg
     8641 aaaacgacat caacattgcc tttaaccagc ttgaagtgca tctgcacaac gagaagggcg
     8701 atgaggtgac ggaagtcaaa cgcgactaca aaggcgatga cccgacgcca gcggtagggt
     8761 aataaccaaa gggggcgaca tttagttgcc ccgagattac tgacaaagtg cgcgttgttc
     8821 atgccggatg cggcgtgacc gccttatccg gcctacaaag tcatgcaaat tcaatatatt
     8881 gaagtttatt ggtgatactg ataagcgtag cgcatcaggc agttttgcgt ttgcccacaa
     8941 tcttagggga catttagcgg ccccatttat ttctcacttt tccgcctcat catcgcgcgt
     9001 taatttcttt tcataatcgc gttttacaat atccagcgcg cgcagcacgg tgcgggctgg
     9061 gatctgattt tcttccagca gcacaatcaa atcgacagcc agtttgacat cgtcaggggc
     9121 attttccagt gacatactct ctccattgct agcgggttaa gcgcgctagc ctgttttcga
     9181 ttttttccag cgcatggcgg cagcgtgcca ggcgcgcttc ataggcttcc acttcacgat
     9241 gcagcgtttg ctgttccacg agatcggtca cccgcgccag gcgggctctg cgttcggcaa
     9301 ccatctcacg tagccgccgc tcaaaatcct gatgctgaat acgtttacgc tgccagcggg
     9361 caattttcgg tggcgcactg tcccactcgc gcaatgacca ggcggaggct tcacgcgcga
     9421 tggcttccag ttgtgccgcc agatgttccg ccagccaggc cacttgcggc agctgttgct
     9481 gctcaactgc atgacgaagc gcagccagat tatcgcccgc ctcatcgaga caagcctgca
     9541 gtgttgtcgc acgggtttga aaaaggtgtc tgtcaaaacg ggcacttagc gtggcaaatt
     9601 gcgctaccgg ggcacaacgc tgacgcagcg tagcgagctg tccttccagt ttttccagca
     9661 gcagggcggt tttcacgata agacctcaat gaaaatgata attgttatgc taaagtagcc
     9721 actctgtaag ctgacaacac tcaatatgca acgaatcatt ttaatcatca ttggctggct
     9781 ggcggtagtg ctgggtacgc tgggcgtggt attaccggta ttaccgacga cgccgtttat
     9841 cctgctggcg gcctggtgct ttgcccgttc ttccccgcgc tttcacgcct ggttgctgta
     9901 ccgctcatgg tttggcagct atctacgttt ctggcagaaa catcatgcga tgccgcgcgg
     9961 cgtcaaaccg cgggcgattt tgcttatttt gctcacgttt gccatttctc tgtggttcgt
    10021 ccagatgcca tgggtgcgca tcatgttgct ggtaattctc gcctgtttgc ttttctatat
    10081 gtggcgaatt ccggtgattg atgaaaagca agaaaagcac tgaagcacaa caatcgcagt
    10141 tgcaattatt gcgtacagcc agtacattct ggcgttttcg agcacaggcg caggcggtca
    10201 aaggttaaac aactgttact tttgatacgt ttaaaacgcg ccgtgagtac caccgtaaca
    10261 agcaggcata cacttatgac cgcgactgca cagcagcttg agtatctcaa aaatagcatc
    10321 aaaagcattc aggactaccc aaaacccggc attcttttcc gcgatgtcac cagcttactg
    10381 gaagacccga aagcttacgc tctcagcatc gacttgctgg ttgagcgtta caaaaatgcg
    10441 ggcattacca aagttgtcgg caccgaagcg cgtggcttct tgtttggcgc tccggtagct
    10501 ctgggtctgg gcgttggctt tgtaccggtc cgtaaaccgg gcaaactgcc gcgtgaaacc
    10561 atcagtgaaa cttacgacct ggaatacggc accgatcagc tggagatcca cgttgatgcc
    10621 atcaaaccgg gcgacaaagt tctggtggtg gacgatctgc tggcaaccgg cggcactatc
    10681 gaagcgaccg ttaaactgat ccgtcgtctg ggtggtgaag tggctgacgc tgcgttcatt
    10741 atcaacctgt tcgatctcgg cggcgaacag cgtctcgaaa aacagggcat taccagctac
    10801 agccttgtcc cgttcccggg ccattaatta tcgccagtct tgtgctgccc acgctacgga
    10861 cagcacaaga tgtgcattca gcctcgccgt tctgacgggg ctgtgttagc attacccctt
    10921 cgtgaatcca ccttccagcg tttcagagcc tgccaatgag ttatcaggtc ttagcccgaa
    10981 aatggcgccc acaaaccttt gctgacgtcg tcggccagga acatgtgctg accgcactgg
    11041 cgaacggctt gtcgttaggg cgtattcatc atgcttatct tttttccggt acccgtggcg
    11101 tcggaaaaac ctctatcgcc cgactgctgg cgaaggggct aaactgcgaa accggcatta
    11161 ccgcgacgcc gtgcggcgtg tgcgataact gtcgtgaaat cgagcagggg cgctttgtcg
    11221 atctgattga aatcgacgcc gcctcgcgca ccaaagttga agatacccgc gacctgctgg
    11281 ataacgtcca gtacgctccg gcgcgtggtc gtttcaaagt ttatctgatc gacgaagtgc
    11341 atatgctgtc gcgccacagc tttaacgcac tgttaaaaac ccttgaagag ccgccggagc
    11401 acgttaagtt tctgctggcg acgaccgatc cacagaaatt gccggtgacg attttgtcgc
    11461 gctgtttgca atttcatctc aaggcgctgg atgtcgagca aattcgccat caacttgagc
    11521 acatcctcaa cgaagaacat atcgctcacg agccgcgggc gttgcaattg ctggcgcgcg
    11581 ccgctgaagg cagcctgcga gatgccttaa gtctgaccga ccaggcgatt gccagcggtg
    11641 acggccaggt ttcaacccag gcagtcagtg cgatgctggg tacgcttgac gacgatcagg
    11701 cgctgtcgct ggttgaagcg atggtcgagg ccaacggcga gcgcgtaatg gcgctgatta
    11761 atgaagccgc tgcccgtggt atcgagtggg aagcgttgct ggtggaaatg ctcggcctgt
    11821 tgcatcgtat tgcgatggta caactttcgc ctgctgcact tggcaacgac atggccgcca
    11881 ttgagctgcg gatgcgtgaa ctggcgcgca ccataccgcc gacggatatt cagctttact
    11941 atcagacgct gttgattggt cgcaaagaat taccgtatgc gccggaccgc cgcatgggcg
    12001 ttgagatgac gctgctgcgc gcgctggcgt tccatccacg tatgcctctg cctgagccag
    12061 aagtgccacg ccagtccttt gcgcccgtcg cgccaacggc agtaatgacg ccaacccagg
    12121 tgccgccgca accgcaatca gcgccgcagc aggcaccgac tgtaccgctc ccggaaacca
    12181 ccagccaggt gctggcggcg cgccagcagt tgcagcgcgt gcagggagca accaaagcaa
    12241 aaaagagtga accggcagcc gctacccgcg cgcggccggt gaataacgct gcgctggaaa
    12301 gactggcttc ggtcaccgat cgcgttcagg cgcgtccggt gccatcggcg ctggaaaaag
    12361 cgccagccaa aaaagaagcg tatcgctgga aggctaccac tccagtgatg cagcaaaaag
    12421 aagtggtcgc cacgccgaag gcgctgaaaa aagcgctgga acatgaaaaa acgccggaac
    12481 tggcggcgaa gctggcggca gaagccattg agcgcgacgc gtgggcggca caggtgagtc
    12541 aactttcgct accaaaactg gtcgaacagg tggccttaaa tgcctggaaa gaggagagcg
    12601 acaacgcagt atgtctgcat ttgcgctcct ctcagcggca tttgaacaac cgcggtgcac
    12661 agcaaaaact ggctgaagcg ttgagcacgt taaaaggttc aacggttgaa ctgactatcg
    12721 ttgaagatga taatcccgcg gtgcgtacgc cgctggagtg gcgtcaggcg atatacgaag
    12781 aaaaacttgc gcaggcgcgc gagtccatta ttgcggataa taatattcag accctgcgtc
    12841 ggttcttcga tgcggagctg gatgaagaaa gtatccgccc catttgatcg taagcacagc
    12901 ttacgttcgt catccttaac gtgattgaga gagaaaccta tgtttggtaa aggcggtctg
    12961 ggtaacctga tgaagcaagc ccagcagatg caagaaaaaa tgcagaaaat gcaggaagag
    13021 atcgcgcagc tggaagtcac cggcgaatct ggcgcaggtc tggtaaaagt gaccatcaac
    13081 ggtgcacaca actgccgtcg cgtagagatc gacccgagcc tgctggaaga cgacaaagag
    13141 atgctggaag acctggtggc tgcagcattc aacgacgcag cacgtcgtat tgaagaaacg
    13201 cagaaagaaa aaatggcctc tgtatcctcc ggaatgcagc tgccgcctgg ctttaagatg
    13261 ccgttctgat gcaaaccagc ccgctgttaa cacagcttat ggaagcactg cgctgtctgc
    13321 cgggcgttgg cccgaagtcg gcgcagcgta tggcgttcac gctgcttcag cgcgatcgta
    13381 gcggcgggat gcgtctggcg caggcgctca cccgggcgat gtcggaaatc ggccactgcg
    13441 ccgattgccg cactttcacc gaacaggaag tctgtaacat ctgttcgaat ccgcgtcgtc
    13501 aggaaaacgg tcaaatctgt gtggtggaga gtccggcgga catctacgcc attgagcaga
    13561 cggggcagtt ttcaggccgt tattttgtgt tgatggggca cctgtcaccg ctggacggca
    13621 tcggtccgga tgatatcggc cttgatcgtc tggaacagcg tctggcagag gaaaaaatca
    13681 ctgaagtgat cctcgccacc aatcccacgg ttgaaggtga agctaccgct aactacattg
    13741 ccgagctttg cgcgcaatat gacgtggaag ccagccgaat cgctcatggc gtaccggttg
    13801 gcggcgaact ggaaatggtc gatggcacca cgctgtcaca ctcccttgcc gggcgtcata
    13861 agattcgttt ttaagcaaac gagagcagga tcacctgctc tcgcttgaaa ttattctccc
    13921 ttgtccccat ctctccctca tcctgttttt aaccttaaaa tggcattatt gaggtagacc
    13981 tacatgaaag gacaagaaac tcgtggtttt cagtcagaag tgaaacagct tctgcacctg
    14041 atgatccatt ctctctattc caataaagaa atcttcctgc gtgagcttat ctctaacgcc
    14101 tccgatgcgg cggacaagct gcgtttccgt gcgctatcta acccggacct gtacgaaggt
    14161 gatggcgaac tgcgcgttcg tgtctctttc gataaagaca agcgcacgct gaccatctcc
    14221 gataacggcg tggggatgac ccgcgacgaa gtgattgacc atctggggac tatcgctaaa
    14281 tccggtacca aatcattcct cgaatccctg ggttctgacc aggcgaaaga cagccagctg
    14341 atcggtcagt ttggtgttgg tttctactct gcgtttatcg tggccgacaa agtgaccgtg
    14401 cgtactcgcg cggcaggcga aaaaccagaa aacggcgtct tctgggaatc ggctggcgaa
    14461 ggtgaatata ccgttgccga catcaccaaa gaagatcgtg gtactgaaat caccctgcat
    14521 ctgcgtgaag gcgaagacga gttcctcgat gactggcgcg tgcgttccat catcagcaaa
    14581 tactccgacc atatcgcgct gccggtagag atcgaaaaac gcgaagagaa agacggcgaa
    14641 accgttatct cctgggagaa aatcaacaaa gcgcaggcgc tgtggactcg taacaagtcg
    14701 gaaatcaccg atgaagagta caaagagttc tacaaacaca tcgcccacga ctttaatgat
    14761 ccgctgacct ggagccacaa ccgcgttgaa ggtaagcagg agtacaccag cctgctgtac
    14821 atcccgtccc aggctccgtg ggatatgtgg aaccgtgatc ataaacacgg cctgaaactg
    14881 tacgtccagc gtgtgttcat catggacgac gcagaacagt tcatgccgaa ctatctgcgc
    14941 ttcgtgcgtg gtctgattga ctccagcgat ctgccgctga acgtttcccg tgaaatcctc
    15001 caggacagca cggtaacgcg taacctgcgc aatgcgctga ccaagcgtgt gctgcaaatg
    15061 ctggaaaaac tggcgaaaga cgacgcggac aaataccaga ccttctggca acagtttggc
    15121 ctggtactga aagaaggtcc ggcggaagat ttcgctaacc aggaagcgat cgccaaactg
    15181 ctgcgttttg cttctactta taccgattct tctgcgcaga ccgtgtctct ggaagactac
    15241 gtttcccgca tgaaagaagg gcaggagaaa atctactaca tcactgcaga cagctatgcg
    15301 gcagcgaaga gcagcccgca cctggaactg ctgcgtaaga aaggcatcga agttctgctg
    15361 ctttccgacc gcatcgatga gtggatgatg aactatctga ctgagttcga cggtaaaccg
    15421 ttccagtcgg tgtctaaagt tgacgagtcg ctggaaaaac tggctgacga agttgatgag
    15481 agcgcgaaag aagcggagaa agcactgact ccgttcatcg accgtgtgaa agccctgctc
    15541 ggcgagcgcg tgaaagatgt ccgtctgact caccgtctga ccgatacgcc agcgatcgtt
    15601 tcgaccgacg cggacgaaat gagcactcag atggcgaaac tgttcgctgc ggcgggccag
    15661 aaagtgccag aagtgaaata catcttcgaa ctgaacccgg atcacgtact ggtgaaacgt
    15721 gcggcagata ctgaagatga agcgaagttc agcgagtggg tagaactgct gctggatcag
    15781 gcgctgctgg cagaacgcgg cacgctggaa gatccgaacc tgtttattcg tcgtatgaac
    15841 cagctgctgg tttcctgatg tcatgccgga tgacctttgt gtcatccggc atttttcttt
    15901 tcatcatctg cactttccgc aaattatctc gccattaacc gtttcagcct caggtgcctt
    15961 tcttgaggca atcgcctgtt ggtggtatcg tttatcgctt tttcaaaaaa ttcgacacat
    16021 tttaagggga ttttcgcaat gcgtatcatt ctgcttggcg ctccgggcgc ggggaaaggg
    16081 actcaggctc agttcatcat ggagaaatat ggtattccgc aaatctccac tggcgatatg
    16141 ctgcgtgctg cggtcaaatc tggctccgag ctgggtaaac aagcaaaaga cattatggat
    16201 gctggcaaat tggtcaccga cgaactggtg atcgcgctgg ttaaagagcg cattgctcag
    16261 gaagactgcc gtaatggttt cctgttggac ggcttcccgc gtaccattcc gcaggcagac
    16321 gcgatgaaag aagcgggcat caatgttgat tacgttctgg aattcgacgt accggacgaa
    16381 ctgattgttg atcgtatcgt aggccgccgc gttcatgcgc cgtctggtcg tgtttatcac
    16441 gttaaattca atccgccgaa agtagaaggc aaagacgacg ttaccggtga agaactgact
    16501 acccgtaaag acgatcagga agaaaccgtg cgtaaacgtc tggttgaata ccatcagatg
    16561 actgcaccgc tgatcggcta ctactccaaa gaagcggaag cgggtaacac caaatacgcg
    16621 aaagttgacg gcaccaagcc ggttgctgaa gttcgcgctg atctggaaaa aatcctcggc
    16681 taattcgaaa gcgcgcacgg acagtcccct cgccccctcg gggagagggt tagggtgagg
    16741 ggaacaggcc cgcacaagca aacttatcag caatctcagg ccggatattc attcggcctt
    16801 ttacaaaaac ctcaatctgc gctattcctt tttctgattt gacctctcac aacaattagt
    16861 tcttcttcct cacttttccg ctacaattat caacaagttg aatcgataag aggcggtaat
    16921 gcgtcagact aaaaccggta tcctgctggc aaacctgggt acgcccgatg cccccacacc
    16981 tgaagcggta aaacgctatc tgaaacaatt tttaagcgac agacgcgtgg ttgatacctc
    17041 acggttgtta tggtggccgt tgctgcgcgg cgtgattttg ccgctgcgct cgccgcgtgt
    17101 ggcgaagatg tacgcctcgg tctggatgga agacggctcg ccgctgatgg tttacagccg
    17161 ccagcaacag caggcgttgg cacaacgttt accggatacg cccgtagcgc tgggcatgag
    17221 ctacggctcg ccatcactgg aaagcgccgt cgatgaactc ctggcagagc atgtagatca
    17281 tattgtggtg ctgccgcttt atccgcaatt ctcctgttct acggtcggtg cggtatggga
    17341 tgaactggca cgcattctgg cgcgcaaacg tagcattccg gggatatcgt ttattcgtga
    17401 ttacgccgat aaccacgatt acattaatgc actggcgaac agcgtacgcg cttcttttgc
    17461 caaacatggc gaaccggatc tgctgctgct ctcttatcat ggcattcccc agcgttatgc
    17521 agatgaaggc gatgattacc cgcaacgttg ccgcacaacg actcgcgaac tggcttccgc
    17581 actggggatg gcaccggaaa aagtgatgat gacctttcag tcgcgctttg gccgggaacc
    17641 ctggctgatg ccttataccg acgaaacgct gaaaatgctc ggagaaaaag gcgtaggtca
    17701 tatacaggtg atgtgcccgg gctttgctgc ggattgtctg gagacgctgg aagagattgc
    17761 cgagcaaaac cgtgaggtct tcctcggtgc cggcgggaaa aaatatgaat atattccagc
    17821 gcttaatgcc acgccggaac atatcgaaat gatggctaat cttgttgccg cgtatcgcta
    17881 aagctgagcg gtaaagaact gagcgccgtc gcgaagagcc tcgtcggcgg ttttcatcat
    17941 tcgtgaataa tgcaaaaagg catgcagcgt acctgggtag agtttgaact cacagggctg
    18001 ctgatgcgcc gctaacgtct ggtaaagcag acggctgtca tccagcagcg gatcgaactc
    18061 cgccccggca ataaaacagg gcggaacttc gcgagtgaga tcattattaa acagacagta
    18121 atacggtgac tcgcggtccg cgtcgttgct taaatacgcc tcttcataca tctgcaaatc
    18181 ctgttgcgtt aagccatccc agacaccgcc caacagacga cgagtcacgg aatcccgtaa
    18241 tccgtaaagc ccataccaca gcaaaacacc cgcaatttta ccgcaatcga tctgtttatc
    18301 acgcaaccac aacgcactgg cgagcgccag catggcacct gcggaatcac cggcaaagcc
    18361 aatgcgggac atattgattt gataatcctc cgcctgctgg tggaaataac aacaagcagc
    18421 cacaatttcc tctatcgctt gcggaaaacg cgcttcaggt gaaagggggt aatcaatacc
    18481 aatcaccgta cattggctgt agcttgccag caggcgcatg atgcgatcgt gggtatcgag
    18541 attgccgaga ataaaaccgc ctccatgcaa ataaaatagc gtcgccgggc tatctggctg
    18601 cggacaaaag agacgtgttt ccacctgccc atattttgtt ggaaccatgt aagctctggt
    18661 tgccatttct ggagcgcccg cattccagaa tcggcgctca agcgtgtaat actgtcgttg
    18721 ctcagcaatc gttcccgttg cgggccagga cggtaaatcc ggctgaagag tattcacaac
    18781 ggtcttcatt tcagcagaaa taaggtccag aacaggtagt ttgttttccg gcttcataca
    18841 aaaactcctt tcaaattacg tcattgtaag gaaccactgc catgaaaatg cgatcccgcc
    18901 tgctgatatt gaaactggct gcgtctcgcg cgctcccgtc agattgtgtt aacattcgcc
    18961 gctcagttaa ccacccgtaa aaacaaccat gaaatttccc ggtaaacgta aatccaaaca
    19021 ttacttccct gtaaatgcac gcgatccgct gcttcagcaa ttccagccag aaaacgaaac
    19081 cagcgccgcc tgggtcgtag gtattgacca gacgctggtc gatattgaag cgaaagtgga
    19141 tgatgaattc attgagcgtt atggattaag cgccgggcat tcactggtga ttgaggatga
    19201 cgtagccgaa gcgctttatc aggagctaaa acagaaaaac ctgattaccc atcagtttgc
    19261 gggtggcacc attggtaaca ccatgcacaa ctactcggtg ctcgcggacg accgttcggt
    19321 gctgctgggc gtcatgtgca gcaatattga aattggcagt tatgcctatc gttacctgtg
    19381 taacacttcc agccgtaccg atcttaacta tctacaaggc gtggatggcc cgattggtcg
    19441 ttgctttacg ctgattggcg agtccgggga acgtaccttt gctatcagtc caggccacat
    19501 gaaccagctg cgggctgaaa gcattccgga agatgtgatt gccggagcct cggcactggt
    19561 tctcacctca tatctggtgc gttgcaagcc gggtgaaccc atgccggaag caaccatgaa
    19621 agccattgag tacgcgaaga aatataacgt accggtggtg ctgacgctgg gcaccaagtt
    19681 tgtcattgcc gagaatccgc agtggtggca gcaattcctc aaagaccacg tttctatcct
    19741 tgcgatgaac gaagatgaag ccgaagcgtt gaccggagaa agcgatccgt tgttggcatc
    19801 tgacaaggcg ctggactggg tagatctggt gctgtgcacc gccgggccaa tcggcttgta
    19861 tatggcgggc tttaccgaag acgaagcgaa acgtaaaacc cagcatccgc tgctgccggg
    19921 cgctatagcg gaattcaacc agtatgagtt tagccgcgcc atgcgccaca aggattgcca
    19981 gaatccgctg cgtgtatatt cgcacattgc gccgtacatg ggcgggccgg aaaaaatcat
    20041 gaacactaat ggagcggggg atggcgcatt ggcagcgttg ctgcatgaca ttaccgccaa
    20101 cagctaccat cgtagcaacg taccaaactc cagcaaacat aaattcacct ggttaactta
    20161 ttcatcgtta gcgcaggtgt gtaaatatgc taaccgtgtg agctatcagg tactgaacca
    20221 gcattcacct cgtttaacgc gcggcttgcc ggagcgtgaa gacagcctgg aagagtctta
    20281 ctgggatcgt taagttatcg tcggttcgta ggccagataa ggcgttcacg ccgcatctgg
    20341 catttggctc tcgatgcctg atgcgacgct ggcgcgtctt atcatgccta catatttttc
    20401 atattttaca tccggcaacc accgtttacc ccgtcaccac ctcacccgcc ggtggcgttt
    20461 ccagcagttc cagcatggta cgggcgattt cacgctcgcc catcactacc tgattcgcac
    20521 cacgttcggt gatatacgcc acttcatcgt cataatgggc gcgggcaata atctcaatat
    20581 ccggattttt cgcgcgggca gatgccacaa tctcacccgc ttcataaccg ttgggaatcg
    20641 tcaggatcag ccattttgca cattccagat gcgccagttg cataatttct tcgttcgccg
    20701 cattgcccaa tactgcgcgg accccgcgct ctcgcagctc atcaacacgg gttcgtgacg
    20761 tctcaatcac caccagcgga atatcagagg cgagcaattt ctcccccagc aggctgccta
    20821 cacgaccgta acccaccagt agcgcatggt tgcaaatatc cactgggatc tgcttctctt
    20881 cttcgattgc ctcttccagc gtctgctctt ccagcgtttc ggtcttcgcc agatatttct
    20941 ccagtagtgc gaacagtacc gggttgagca taatcgacag gatcgcccct gccagtacta
    21001 ggttttgtcc ggcctgcggc agtaaattca atgccattcc cagtcccgcc aggataaacg
    21061 cgaactcacc aatctgcgcc aggctggcgg cgatggttaa tgccgtacgt tgggagtgac
    21121 caaacagtcg caccaggaaa aatgcggcta acgacttacc aaacagaata atcgccagcg
    21181 tcgccagcac tgccagcggt tgctgaatca gaattaacgg atcaaacaac atcccgacgg
    21241 agacaaaaaa cagcaccgca aacgcgtcgc gcaatggcag cgtatcgtgg gcggcacggt
    21301 gactcagttc agactcgttc agtaccatcc cggcaaagaa cgcaccgagt gcaaaggaga
    21361 catcaaacag ctctaccgca ccaaaggcaa tccctaacgc cagcgccagc accgacaggg
    21421 taaacagctc gcgagaaccg gttgccgcgc tgcgtgccat aatccacggc accagacggc
    21481 gacctaccag catcataatg gcgataaatg cgatcacttt gccgatggtg atccccatat
    21541 cgactgcaag agtggcaaag cccacatcgc cctgttccat cattcctgcc actgcgggca
    21601 gcaacaccag cgtcagaacc attaccaggt cttccacaat caaccaaccg atggcgattt
    21661 gcccacgctg actgtcaatt aattgccgtt cttcaagtgc gcgcagtaac accacggtac
    21721 tggcggtgga aagacataaa ccgaacacga taccggtcat taacgaccag cccagcacgg
    21781 cggagagcgc catacccagc agcgtcgcca cggctatctg ggcgatcgcg ccgggaatgg
    21841 cgatggactt taccgccatc aaatccttga gcgaaaaatg caaaccgacg ccgaacatca
    21901 gcaaaataac gcccagttca gccagttccg gcgcaagctt ggtatcggca acaaagcccg
    21961 gagtgaatgg tcctgccagc acacccgcta acagatatcc caccagagga gaaatacgta
    22021 gtttattggc cagcatgccg aggataaagg cgagcacaag gccgccaaca atggtggtga
    22081 taagcggggt ggcgtgatgc attccgtctc cttttcctgg tggttattgt ccatttttgg
    22141 ccgggaaaac caaaattaca ggtaatagtt tatgacaatt tcattgatga tgttcatgaa
    22201 taattattga attttgcaga aaaatggaat tagctgcaaa aaaagcacgg atcggaaaac
    22261 agaaggcggt ttaacagagg aaaggcttat ggcgactgta aaacgatgca gccaaagttt
    22321 gcctttggct gcaatgaaat cagtctttat gccggttatc aggcaggaat atggtcaaca
    22381 tccccaatag tggcaggaaa gcacagattt tatagactaa ctcgatactg gtgtgatcgg
    22441 cgataagccc cagaactgcc gctcccagac ctcccatacc aaaagcaaaa ccgaaaaaga
    22501 gtccagaaac cataccgata cgtcccggaa gcagctcctg agcgtaaacc agaatggcag
    22561 agaatgccga agcgaggata aatccaataa tcaccgttaa aacccccgtc cagtgcaggc
    22621 tggcgtaggg taaaatcagc gtaaacggcg caacgccgag gatagagccc caaatcacat
    22681 atttccgccc aattttatcc cctacaggcc cgccgatcac cgtacctgcc gcaacggcaa
    22741 acaggaaggc aaacagatga agctgagcat tctggataga taatccgaat ttttgcatca
    22801 gataaaaggt gtaatagctg ctgatgctcg ccatatagaa atatttcgag aaaatgagga
    22861 ttaacagaat gctgaccgcc agtaccactt tgttgcgcgg cagtggattg ataatcgtcg
    22921 ctttgggttt tcctttattc attcggtgct gtgccgagta ccaacggctg atttgcgcca
    22981 acaccacgat cgccagcagt gccgcaagca caaaccaggc aacgttgcct ttgccataag
    23041 gcgcgataat caccgccgcc agcaagggtc ccagggaact gccaaagttg ccgccgacct
    23101 gaaagataga ttgcgccagg ccatgccgcc cgccggaagc catacgggct acgcgagaag
    23161 attccggatg aaagaccgat gaaccggtac cgaccagcgc cgccgccagc agaactgcgc
    23221 caaaactgcc cgccagcgca agcagcacca gaccacttaa ggtaaagcac atgccaattg
    23281 gcaacgacca tggcatcgga tatttatcgg tccagtagcc gaccactggt tgcagtagcg
    23341 aagaggcgag ctggaaggtg agggttatca tgccaatctg cataaatgtc agagaaaatt
    23401 ctgactgaag cagcggataa atcgccagaa tcagcgattg gatcatgtcg ttcagcagat
    23461 gtgagaggct gatagcacct aaaataccaa acgatgttcg ggccttggtc gttgacgcag
    23521 ccgcgcccgc cacaggctgg ggttgttcac tcattgccat aggaaagtca ctttttcagg
    23581 gttgcgatgt aaagaatgat cttatttgtg attattaccc gactaacata cctgtatgcg
    23641 tcgcctgaag gaagtctcaa cgccgaatac agaatttcta atctggatgc agatttatct
    23701 tcaccggacg cagacttgtc tatgatgtcg cgtcatacta tttttcaaca cgttgaaatc
    23761 aggtcaggga gagaagtatg aaattattgc agcggggcgt ggcgttagcg ctgttaacca
    23821 catttacact ggcgagtgaa actgctctgg cgtatgagca ggataaaacc tacaaaatta
    23881 cagttctgca taccaatgat catcatgggc atttttggcg caatgaatat ggcgaatatg
    23941 gtctggcggc gcaaaaaacg ctggtggatg gtatccgcaa agaggttgcg gctgaaggcg
    24001 gtagcgtgct gctactttcc ggtggcgaca ttaacactgg cgtgcccgag tctgacttac
    24061 aggatgccga acctgatttt cgcggtatga atctggtggg ctatgacgcg atggcgatcg
    24121 gtaatcatga atttgataat ccgctcaccg tattacgcca gcaggaaaag tgggccaagt
    24181 tcccgttgct ttccgcgaat atctaccaga aaagtactgg cgagcgcctg tttaaaccgt
    24241 gggcgctgtt taagcgtcag gatctgaaaa ttgccgtcat tgggctgaca accgatgaca
    24301 cagcaaaaat tggtaacccg gaatacttca ctgatgtcga atttcgtaag cccgccgatg
    24361 aagcgaagct ggtgattcag gagctgcaac agacagaaaa gccagacatt attatcgcgg
    24421 cgacccatat ggggcattac gataatggtg agcacggctc taacgcaccg ggcgatgtgg
    24481 agatggcacg cgcgctgcct gccggatcgc tggcgatgat cgtcggtggt cactcgcaag
    24541 atccggtctg catggcggca gaaaacaaaa aacaggtcga ttacgtgccg ggtacgccat
    24601 gcaagccaga tcaacaaaac ggcatctgga ttgtgcaggc gcatgagtgg ggcaaatacg
    24661 tgggacgggc tgattttgag tttcgtaatg gcaaaatgaa aatggttaac taccagctga
    24721 ttccggtgaa cctgaagaag aaagtgacct gggaagacgg gaaaagcgag cgcgtgcttt
    24781 acactcctga aatcgctgaa aaccagcaaa tgatctcgct gttatcaccg ttccagaaca
    24841 aaggcaaagc gcagctggaa gtgaaaatag gcgaaaccaa tggtcgtctg gaaggcgatc
    24901 gtgacaaagt gcgttttgta cagaccaata tggggcggtt gattctggca gcccaaatgg
    24961 atcgcactgg tgccgacttt gcggtgatga gcggaggcgg aattcgtgat tctatcgaag
    25021 caggcgatat cagctataaa aacgtgctga aagtgcagcc attcggcaat gtggtggtgt
    25081 atgccgacat gaccggtaaa gaggtgattg attacctgac cgccgtcgcg cagatgaagc
    25141 cagattcagg tgcctacccg caatttgcca acgttagctt tgtggcgaaa gacggcagac
    25201 tgaacgacct taaaatcaaa ggcgaaccgg tcgatccggc gaaaacttac cgtatggcga
    25261 cattaaactt caatgccacc ggcggtgatg gatatccgcg ccttgataac aaaccgggct
    25321 atgtgaatac cggctttatt gatgccgaag tgctgaaagc gtatatccag aaaagctcgc
    25381 cgctggatgt gagtgtttat gaaccgaaag gtgaggtgag ctggcagtaa tccgaaagtg
    25441 ccggatgttt gcatccggca caatgcttaa tcgcggcggg cgatatcagc aaatttggca
    25501 tcgaggatct ttgccagatc gcctgccgcc agttcgatat ccagtccgcg cttgccgccg
    25561 gaaacataaa tagtggcaaa ttcttgtgcg ggggcgtcga taatcgttgg cagacgtttt
    25621 ttctgcccca gtgggctaat ccccccaacc aggtatcccg tcgaacgctg cgcgaccatc
    25681 ggatcggcca tctcaacttt cttggcaccc agcgcttttg ctactttttt aagatccagt
    25741 tgaccggcga ccggcgtaac ggccacggca aggtgtttca tatcaccgtt cactgccacc
    25801 agcagcgttt tgtagacctg atccggattc aaacctaatt ttttgacgac ttcatcgcca
    25861 aaattggttt cagccggatc gtgctcgtag gtatggattt gaaacgaaat cttgtttttt
    25921 tcgagtaatt taactgcggg tgtcatggta attttcctgc cttaaacaaa aaaacgaagc
    25981 aagcatacgc ctgatggcgg tccaaaaaaa agagtcatct tgcctaagag tattggcagg
    26041 atggtgagat tgagcgacaa tcgagttaca ccgtcgctta aagtgacggc ataataataa
    26101 aaaaatgaaa ttcctctttg acgggccaat agcgatattg gccatttttt tagcgcaaca
    26161 tttgcggtaa attcccttct ccatacaggt gtagtgcacc gaccgcgacc acatatcgcc
    26221 ccggcggcat ggcgcgtaat ttatcccgcc aggcgagatt tcgctgatgc atcagcacat
    26281 cgtacagcga ctgactgaac gtattgggca gcgttatttc attattttgc ggcggtgcat
    26341 tcagccacca gctcatcatt tgttgcagca accgtgcatt ggtgtgccag tgggtcagtg
    26401 tatcgtccag cagcgccagt cctttgtcag ggagctggag caacatggca atctggtttt
    26461 cagccccttc cagttcaatc acgggtttat gttgttgctt cgccgcctgc aatagctggt
    26521 aatcgatacc gtattctgcc cgcagcccca gtttttgtgc ctgcgtcgcc tgaagaacca
    26581 tcgcgatttg ccacagcggt tgggtagaaa agagtgaggg agaaatgccc atctcctggc
    26641 taatgtgctg cagattttgg agttgctcct cgctaatgcg ctcttccagc gcctcgcagg
    26701 caggcaaatt ggcaaaaggc gtatcgctgg tggaaacatc cgcctcgacg atcagcgcat
    26761 cggcgttttt gagctttttg agcaaacggg tgggcagggg agccatatcg tggctaccca
    26821 tatgaatact gccaatcaga tgaaaatggc gattgccggg aagggtgata tcgatggcag
    26881 gccaggtgta atgattaccg cgcagcgcgg cccaaagcgt ttttacccgg tacaacagat
    26941 ccatacgacc tccctttgtg aaatatcatg ctagcgcgcg gggagggatg gtgcaaatgg
    27001 agttggggac ttgtttaatg tgtttgtatg attcagtata ttcttgtatc gctattcaca
    27061 gggaagcaac agttaaaaac tatgaaacag gcaacaagaa aaccgacgac acccggagat
    27121 attcttctct atgaatatct ggtaccgctc gatttgaaaa tcaatgagtt agcagagttg
    27181 ctgcatgttc atcgtaatag cgtcagtgca ctgatcaata acaatcgtaa actcaccact
    27241 gagatggcat tccgtctggc gaaagttttt gataccacag tcgatttttg gctaaacctc
    27301 caggcggcag ttgatctttg ggaagttgaa aacaacatgc gcacccagga agaattggga
    27361 cggattgaaa cagtggctga atacttggca cgccgtgaag agcgtgcaaa aaaggtcgcg
    27421 taaggccaaa acatcgcttc atctcgtagg ccggataagg cgtttacgcc gcatccggca
    27481 gtcatgcgtc gatgccaaat gtgccaccct aaagcagcgc atccgcaatg acatacttat
    27541 tccttcggtt taaaccgcag caaccggttg gcgttactca ctacggtaat cgacgagagc
    27601 gccattgccg ctccggcaac taccgggtta agcagtgttc cagtaaacgg ccacaaaata
    27661 ccggcggcga ccggaatacc gatactgttg tagataaagg caccgagcag gttctgcttc
    27721 atgttgcgca gcgttgcgcg ggaaatcgcg agcgcatccg caacgcccat caggctatgg
    27781 cgcatcaggg taatcgccgc ggtttcaatg gcaacatcac tgccgccccc catcgcaata
    27841 ccgacatccg cctgggccag cgctggcgca tcgttaatgc cgtcgcccac cattgccacc
    27901 tgacgtcctt cactttgcag gcgtttaatc gcttcggctt taccgtccgg cagcaccccg
    27961 gcgatcactt catcaatccc ggcttctttg gcgatcgcat tggcggtggt tgggttatcc
    28021 ccggtcaaca tcaccagacg atatcccgct ttatgcaggc gttgcagcgc cgccacgcta
    28081 tcactacgca atggatcgcg tactgccagc agggctaccg ctttcccgtc aaccgccagc
    28141 agcacaggcg ttgccccttg cgatgcctga gcagtaatct ccgcttcgat agctttggta
    28201 ccaacctgtt gctcatttaa cagcgcctga ttgcccagca ataacgcatg accttcagct
    28261 tcaccgctca cgcccagccc gcgcaatgtg cggaaaccgt tgacctgcgg cagctgcata
    28321 tcacctgctt tatcgaggat cgcccgtgcc agcggatggc tggaaccttg ctccagtgcc
    28381 gccgccagac gcaatgcctg cgcttcatca acatcagcaa acgttttcac tgcgacaacc
    28441 tgcggcttcc cttcggtcag cgtcccggtt ttatcgaaca cgacggtatc gagtgtactg
    28501 gcgcgttgca gcgcgtcggc gtcccgcacc agcacgccaa actcagccgc ccgcccgacg
    28561 ccggaaataa tcgacatcgg cgtcgccagc cccagcgcac acggacaggc gataatcagt
    28621 accgtggtgg caatcaccag ggtataaaca atttgcggtg ccggaccaaa gaagtaccag
    28681 attgccgcac tgaccagcgc aatcaccacc accacaggta caaatacggc tgagatttta
    28741 tccgccagct gaccgatttc tggcttgctg ctctgggcct ggcgcaccat gcgaatgatc
    28801 cgtgacagcg tggtatggct gccaaccgca ctggcacgaa acagcacgct accgtcctgt
    28861 accactgtcc cggcatggac gctttcgcct tcgccttttt gctgcgggat tggttcgccc
    28921 gtcagcatcg cttcatccag ccatgcttcg ccctgggtaa tctcgccatc taccggcacg
    28981 cgatcgccgg tcgtcaggcg cagcaacata cctggctgca cttccgccag aggcacgctt
    29041 ttttcacctt cgtcagtaac caggcgcgcc gtcggtgggg ttaaatcgag taatttctcc
    29101 agcgccttag aagaacgctg acgtgcgcgt gcttccagca tatggccgag attgatcaga
    29161 ccgataatca tcgcgctggc ttcgtaataa agatgtcgcg cttccatcgg gaaccactgc
    29221 ggccacaggt tgacgctcat cgaatagagc cacgccacgc cagtacccag cgccaccagc
    29281 gtatccatcg tcgctgcacc gttcagcagg cttttccatg cactgcggta aaaatggccg
    29341 ccggcgaaaa ccatcaccgc cagggttatc aggccgataa ccaaccacag gctgcggttg
    29401 tcagcggtga ccatcatgtt atcgccaatc atcccccaga ccatcaccgg gatacccacc
    29461 gccagtgcga caattgcctg ccagcggaag cgcttcatcg tagcaacggc ggtttcctgc
    29521 tggcgctcac ggcgtttcgc gtcatcttca atcgcttccg cgccgtagcc cgctttttcc
    29581 accgcctgca ctaaatcttg tggggaggca ctgcccatca ccagcgcagt acgctccgcc
    29641 aggtttaccc gtgcctgagt gacgcccggt acgctttgca gcgcattttg tacgcgggtg
    29701 acacagctgg cgcagctcat gccgctcagc agcaactgct ggctgtcatc gtcatcggcg
    29761 gtcgctgccg gaagcgcctc agaaaccgct gtcagtgctt ccgacgggat tgatgactcc
    29821 gccagcggtt tagcctttgg gtggcttaca gatgcgtcat aacccgcttg tttgatggtt
    29881 tcgatcagct gttctgcact ggcagtcccg gtaacgtgcg cttcagtgat agacacatcc
    29941 gcttgttcca catccgggcg ctgttcgaga ctttctttca cgcgtttaac gcagtgaccg
    30001 caggacaggc cgtccagggt caggtcgata atttgtgaca taaaacactc ctttaagaca
    30061 gttttgactg gctgtgataa aggttaaacc ttccagcaag gggaaggtca agaaattaat
    30121 aaaccaggcg agtaaaagtc cgtaaagatt aaaaaattgg ctcaatttgc atcaggatta
    30181 gacatttatc tctttgtttt cctgtagtta agttgcgggt gctaagttaa atcaagatgc
    30241 ctgaaaatcg gcaccggggt gaggaattac ctcccgcatc tataaaaagg agttaacaaa
    30301 agatgttaga tgcaaacaaa ttacagcagg cagtggatca ggcttacacc caatttcact
    30361 cacttaacgg cggacaaaat gccgactaca ttccctttct ggcgaatgta cctagtcaac
    30421 tggcagcagt ggctattgtg acctgcgatg gcaacatcta tcgcgcgggt gatagtgatt
    30481 accgctttgc gctggaatcc atctcgaaag tctgtacgtt agcccttgcg ttagaagatg
    30541 tcggcccgca ggcggtacag gacaaaattg gcgctgaccc gaccggattg ccctttaact
    30601 cagttatcgc cttagagttg catggcggca aaccactgtc gccactggta aatgctggcg
    30661 ctattgccac caccagcctg attaacgctg aaaatattga acaacgctgg cagcgaattt
    30721 tacatatcca acagcaactg gctggtgagc aggtagcgct ctctgatgaa gtcaaccagt
    30781 cggaacaaac aaccaacttc cataaccggg ccattgcctg gctgctgtac tccgccggat
    30841 atctctattg tgatgcaatg gaagcctgtg acgtgtacac ccgtcagtgc tccacgctca
    30901 tcaatactgt tgaactggca acgcttggcg cgacgctggc ggcgggtggt gtgaatccgc
    30961 tgacgcataa acgcgttctt caggccgaca acgtgccgta cattctggcc gaaatgatga
    31021 tggaagggct gtatggtcgc tccggtgact gggcgtatcg cgttggttta ccgggcaaaa
    31081 gcggtgtagg tggcggtatt ctggcggtcg tccctggcgt gatgggaatt gccgcgttct
    31141 caccaccgct ggacgaagaa ggcaacagtg ttcgcggtca aaaaatggtg gcatcggtcg
    31201 ctaagcaact cggctataac gtgtttaagg gctgatcatg atgaacacgg aaggtaataa
    31261 cggtaacaaa cctctcggtc tatggaacgt cgtttccatc ggcattgg
//
LOCUS       DAZKHF020000023        31081 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2201S31241
            SAMN43267800-rid25106413.denovo.023, whole genome shotgun sequence.
ACCESSION   DAZKHF020000023 DAZKHF020000000
VERSION     DAZKHF020000023.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267800
            Sequence Read Archive: SRR30307263
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 31081)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 31081)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 31081)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350581.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350581.2
            Genome Coverage       :: 151x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:21:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,652
            CDSs (total)                      :: 5,557
            Genes (coding)                    :: 5,320
            CDSs (with protein)               :: 5,320
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 237
            CDSs (without protein)            :: 237
            Pseudo Genes (ambiguous residues) :: 0 of 237
            Pseudo Genes (frameshifted)       :: 102 of 237
            Pseudo Genes (incomplete)         :: 130 of 237
            Pseudo Genes (internal stop)      :: 52 of 237
            Pseudo Genes (multiple problems)  :: 41 of 237
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..31081
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267800-rid25106413.denovo.023"
                     /serotype="O 26:H11"
                     /isolate="2201S31241"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-01"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            <1..231
                     /gene="tuf"
                     /locus_tag="ACHOSC_RS04515"
                     /old_locus_tag="pgaptmp_000903"
     CDS             <1..231
                     /gene="tuf"
                     /locus_tag="ACHOSC_RS04515"
                     /old_locus_tag="pgaptmp_000903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312930.1"
                     /note="EF-Tu; promotes GTP-dependent binding of
                     aminoacyl-tRNA to the A-site of ribosomes during protein
                     biosynthesis; when the tRNA anticodon matches the mRNA
                     codon, GTP hydrolysis results; the inactive EF-Tu-GDP
                     leaves the ribosome and release of GDP is promoted by
                     elongation factor Ts; many prokaryotes have two copies of
                     the gene encoding EF-Tu; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Tu"
                     /protein_id="HGU4546891.1"
                     /translation="RHTPFFKGYRPQFYFRTTDVTGTIELPEGVEMVMPGDNIKMVVT
                     LIHPIAMDDGLRFAIREGGRTVGAGVVAKVLS"
     gene            461..844
                     /gene="secE"
                     /locus_tag="ACHOSC_RS04520"
                     /old_locus_tag="pgaptmp_000904"
     CDS             461..844
                     /gene="secE"
                     /locus_tag="ACHOSC_RS04520"
                     /old_locus_tag="pgaptmp_000904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007665874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecE"
                     /protein_id="HGU4546892.1"
                     /translation="MSANTEAQGSGRGLEAMKWVVVVALLLVAIVGNYLYRDIMLPLR
                     ALAVVILIAAAGGVALLTTKGKATVAFAREARTEVRKVIWPTRQETLHTTLIVAAVTA
                     VMSLILWGLDGILVRLVSFITGLRF"
     gene            846..1391
                     /gene="nusG"
                     /locus_tag="ACHOSC_RS04525"
                     /old_locus_tag="pgaptmp_000905"
     CDS             846..1391
                     /gene="nusG"
                     /locus_tag="ACHOSC_RS04525"
                     /old_locus_tag="pgaptmp_000905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005280494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0032784 - regulation of DNA-templated
                     transcription elongation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination
                     protein NusG"
                     /protein_id="HGU4546893.1"
                     /translation="MSEAPKKRWYVVQAFSGFEGRVATSLREHIKLHNMEDLFGEVMV
                     PTEEVVEIRGGQRRKSERKFFPGYVLVQMVMNDASWHLVRSVPRVMGFIGGTSDRPAP
                     ISDKEVDAIMNRLQQVGDKPRPKTLFEPGEMVRVNDGPFADFNGVVEEVDYEKSRLKV
                     SVSIFGRATPVELDFSQVEKA"
     gene            1550..1978
                     /gene="rplK"
                     /locus_tag="ACHOSC_RS04530"
                     /old_locus_tag="pgaptmp_000906"
     CDS             1550..1978
                     /gene="rplK"
                     /locus_tag="ACHOSC_RS04530"
                     /old_locus_tag="pgaptmp_000906"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006121495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11"
                     /protein_id="HGU4546894.1"
                     /translation="MAKKVQAYVKLQVAAGMANPSPPVGPALGQQGVNIMEFCKAFNA
                     KTDSIEKGLPIPVVITVYADRSFTFVTKTPPAAVLLKKAAGIKSGSGKPNKDKVGKIS
                     RAQLQEIAQTKAADMTGADIEAMTRSIEGTARSMGLVVED"
     gene            1982..2686
                     /gene="rplA"
                     /locus_tag="ACHOSC_RS04535"
                     /old_locus_tag="pgaptmp_000907"
     CDS             1982..2686
                     /gene="rplA"
                     /locus_tag="ACHOSC_RS04535"
                     /old_locus_tag="pgaptmp_000907"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L1"
                     /protein_id="HGU4546895.1"
                     /translation="MAKLTKRMRVIREKVDATKQYDINEAIALLKELATAKFVESMDV
                     AVNLGIDARKSDQNVRGATVLPHGTGRSVRVAVFTQGANAEAAKAAGAELVGMEDLAD
                     QIKKGEMNFDVVIASPDAMRVVGQLGQVLGPRGLMPNPKVGTVTPNVAEAVKNAKAGQ
                     VRYRNDKNGIIHTTIGKVDFDADKLKENLEALLVALKKAKPTQAKGVYIKKVSISTTM
                     GAGVAVDQAGLSASVN"
     gene            3099..3596
                     /gene="rplJ"
                     /locus_tag="ACHOSC_RS04540"
                     /old_locus_tag="pgaptmp_000908"
     CDS             3099..3596
                     /gene="rplJ"
                     /locus_tag="ACHOSC_RS04540"
                     /old_locus_tag="pgaptmp_000908"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002438625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L10"
                     /protein_id="HGU4546896.1"
                     /translation="MALNLQDKQAIVAEVSEVAKGALSAVVADSRGVTVDKMTELRKA
                     GREAGVYMRVVRNTLLRRAVEGTPFECLKDAFVGPTLIAYSMEHPGAAARLFKEFAKA
                     NAKFEVKAAAFEGELIPASQIDRLATLPTYEEAIARLMATMKEASAGKLVRTLAAVRD
                     AKEAA"
     gene            3663..4028
                     /gene="rplL"
                     /locus_tag="ACHOSC_RS04545"
                     /old_locus_tag="pgaptmp_000909"
     CDS             3663..4028
                     /gene="rplL"
                     /locus_tag="ACHOSC_RS04545"
                     /old_locus_tag="pgaptmp_000909"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005122872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L7/L12"
                     /protein_id="HGU4546897.1"
                     /translation="MSITKDQIIEAVAAMSVMDVVELISAMEEKFGVSAAAAVAVAAG
                     PVEAAEEKTEFDVILKAAGANKVAVIKAVRGATGLGLKEAKDLVESAPAALKEGVSKD
                     DAEALKKALEEAGAEVEVK"
     gene            4348..8376
                     /gene="rpoB"
                     /locus_tag="ACHOSC_RS04550"
                     /old_locus_tag="pgaptmp_000910"
     CDS             4348..8376
                     /gene="rpoB"
                     /locus_tag="ACHOSC_RS04550"
                     /old_locus_tag="pgaptmp_000910"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004870488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta"
                     /protein_id="HGU4546898.1"
                     /translation="MVYSYTEKKRIRKDFGKRPQVLDVPYLLSIQLDSFQKFIEQDPE
                     GQYGLEAAFRSVFPIQSYSGNSELQYVSYRLGEPVFDVQECQIRGVTYSAPLRVKLRL
                     VIYEREAPEGTVKDIKEQEVYMGEIPLMTDNGTFVINGTERVIVSQLHRSPGVFFDSD
                     KGKTHSSGKVLYNARIIPYRGSWLDFEFDPKDNLFVRIDRRRKLPATIILRALNYTTE
                     QILDLFFEKVIFEIRDNKLQMELVPERLRGETASFDIEANGKVYVEKGRRITARHIRQ
                     LEKDDVKLIEVPVEYIAGKVVAKDYIDESTGELICAANMELSLDLLAKLSQSGHKRIE
                     TLFTNDLDHGPYISETLRVDPTNDRLSALVEIYRMMRPGEPPTREAAESLFENLFFSE
                     DRYDLSAVGRMKFNRSLLREEIEGSGILSKDDIIDVMKKLIDIRNGKGEVDDIDHLGN
                     RRIRSVGEMAENQFRVGLVRVERAVKERLSLGDLDTLMPQDMINAKPISAAVKEFFGS
                     SQLSQFMDQNNPLSEITHKRRISALGPGGLTRERAGFEVRDVHPTHYGRVCPIETPEG
                     PNIGLINSLSVYAQTNEYGFLETPYRKVTDGVVTDEIHYLSAIEEGNYVIAQANSNLD
                     EEGHFVEDLVTCRSKGESSLFSRDQVDYMDVSTQQVVSVGASLIPFLEHDDANRALMG
                     ANMQRQAVPTLRADKPLVGTGMERAVAVDSGVTAVAKRGGVVQYVDASRIVIKVNEDE
                     MYPGEAGIDIYNLTKYTRSNQNTCINQMPCVSLGEPVERGDVLADGPSTDLGELALGQ
                     NMRVAFMPWNGYNFEDSILVSERVVQEDRFTTIHIQELACVSRDTKLGPEEITADIPN
                     VGEAALSKLDESGIVYIGAEVTGGDILVGKVTPKGETQLTPEEKLLRAIFGEKASDVK
                     DSSLRVPNGVSGTVIDVQVFTRDGVEKDKRALEIEEMQLKQAKKDLSEELQILEAGLF
                     SRIRAVLVAGGVEAEKLDKLPRDRWLELGLTDEEKQNQLEQLAEQYDELKHEFEKKLE
                     AKRRKITQGDDLAPGVLKIVKVYLAVKRRIQPGDKMAGRHGNKGVISKINPIEDMPYD
                     ENGTPVDIVLNPLGVPSRMNIGQILETHLGMAAKGIGDKINAMLKQQQEVAKLREFIQ
                     RAYDLGADVRQKVDLSTFSDEEVMRLAENLRKGMPIATPVFDGAKEAEIKELLKLGDL
                     PTSGQIRLYDGRTGEQFERPVTVGYMYMLKLNHLVDDKMHARSTGSYSLVTQQPLGGK
                     AQFGGQRFGEMEVWALEAYGAAYTLQEMLTVKSDDVNGRTKMYKNIVDGNHQMEPGMP
                     ESFNVLLKEIRSLGINIELEDE"
     gene            8453..12676
                     /gene="rpoC"
                     /locus_tag="ACHOSC_RS04555"
                     /old_locus_tag="pgaptmp_000911"
     CDS             8453..12676
                     /gene="rpoC"
                     /locus_tag="ACHOSC_RS04555"
                     /old_locus_tag="pgaptmp_000911"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta'"
                     /protein_id="HGU4546899.1"
                     /translation="MKDLLKFLKAQTKTEEFDAIKIALASPDMIRSWSFGEVKKPETI
                     NYRTFKPERDGLFCARIFGPVKDYECLCGKYKRLKHRGVICEKCGVEVTQTKVRRERM
                     GHIELASPTAHIWFLKSLPSRIGLLLDMPLRDIERVLYFESYVVIEGGMTNLERQQIL
                     TEEQYLDALEEFGDEFDAKMGAEAIQALLKSMDLEQECEQLREELNETNSETKRKKLT
                     KRIKLLEAFVQSGNKPEWMILTVLPVLPPDLRPLVPLDGGRFATSDLNDLYRRVINRN
                     NRLKRLLDLAAPDIIVRNEKRMLQEAVDALLDNGRRGRAITGSNKRPLKSLADMIKGK
                     QGRFRQNLLGKRVDYSGRSVITVGPYLRLHQCGLPKKMALELFKPFIYGKLELRGLAT
                     TIKAAKKMVEREEAVVWDILDEVIREHPVLLNRAPTLHRLGIQAFEPVLIEGKAIQLH
                     PLVCAAYNADFDGDQMAVHVPLTLEAQLEARALMMSTNNILSPANGEPIIVPSQDVVL
                     GLYYMTRDCVNAKGEGMVLTGPKEAERLYRSGLASLHARVKVRITEYEKDANGELVAK
                     TSLKDTTVGRAILWMIVPKGLPYSIVNQALGKKAISKMLNTCYRILGLKPTVIFADQI
                     MYTGFAYAARSGASVGIDDMVIPEKKHEIISEAEAEVAEIQEQFQSGLVTAGERYNKV
                     IDIWAAANDRVSKAMMDNLQTETVINRDGQEEKQVSFNSIYMMADSGARGSAAQIRQL
                     AGMRGLMAKPDGSIIETPITANFREGLNVLQYFISTHGARKGLADTALKTANSGYLTR
                     RLVDVAQDLVVTEDDCGTHEGIMMTPVIEGGDVKEPLRDRVLGRVTAEDVLKPGTADI
                     LVPRNTLLHEQWCDLLEENSVDAVKVRSVVSCDTDFGVCAHCYGRDLARGHIINKGEA
                     IGVIAAQSIGEPGTQLTMRTFHIGGAASRAAAESSIQVKNKGSIKLSNVKSVVNSSGK
                     LVITSRNTELKLIDEFGRTKESYKVPYGAVLAKGDGEQVAGGETVANWDPHTMPVITE
                     VSGFVRFTDMIDGQTITRQTDELTGLSSLVVLDSAERTAGGKDLRPALKIVDAQGNDV
                     LIPGTDMPAQYFLPGKAIVQLEDGVQISSGDTLARIPQESGGTKDITGGLPRVADLFE
                     ARRPKEPAILAEISGIVSFGKETKGKRRLVITPVDGSDPYEEMIPKWRQLNVFEGERV
                     ERGDVISDGPEAPHDILRLRGVHAVTRYIVNEVQDVYRLQGVKINDKHIEVIVRQMLR
                     KATIVNAGSSDFLEGEQVEYSRVKIANRELEANGKVGATYSRDLLGITKASLATESFI
                     SAASFQETTRVLTEAAVAGKRDELRGLKENVIVGRLIPAGTGYAYHQDRMRRRAAGEA
                     PAAPQVTAEDASASLAELLNAGLGGSDNE"
     gene            12889..13428
                     /gene="yjaZ"
                     /locus_tag="ACHOSC_RS04560"
                     /old_locus_tag="pgaptmp_000912"
     CDS             12889..13428
                     /gene="yjaZ"
                     /locus_tag="ACHOSC_RS04560"
                     /old_locus_tag="pgaptmp_000912"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heat-shock protein C"
                     /protein_id="HGU4546900.1"
                     /translation="MKQEVEKWRPFGHPDGDIRDLSFLDAHQAVYVQHHEGKEPLEYR
                     FWVTYSLHCFTKDYEHQTNEEKQSLMYHAPKESRPFCQHRYNLARTHLKRTILALPES
                     NVIHAGYGSYAVIEVDLDGGDKAFYFVAFRAFREKKKLRLHVTSAYPISEKQKGKSVK
                     FFTIAYNLLRNKQLPQPSK"
     gene            complement(13838..14971)
                     /gene="thiH"
                     /locus_tag="ACHOSC_RS04565"
                     /old_locus_tag="pgaptmp_000913"
     CDS             complement(13838..14971)
                     /gene="thiH"
                     /locus_tag="ACHOSC_RS04565"
                     /old_locus_tag="pgaptmp_000913"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016829 - lyase activity [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-iminoacetate synthase ThiH"
                     /protein_id="HGU4546901.1"
                     /translation="MKTFSDRWRQLDWDDIRLRINGKTAADVERALNASQLTRDDMMA
                     LLSPAASGYLEQLAQRAQRLTRQRFGNTVSFYVPLYLSNLCANDCTYCGFSMSNRIKR
                     KTLDEADIARESAAIREMGFEHLLLVTGEHQAKVGMDYFRRHLPALREQFSSLQMEVQ
                     PLAETEYAELKQLGLDGVMVYQETYHEATYARHHLKGKKQDFFWRLETPDRLGRAGID
                     KIGLGALIGLSDNWRVDCYMVAEHLLWLQQHYWQSRYSVSFPRLRPCTGGIEPASIMD
                     ERQLVQTICAFRLLAPEIELSLSTRESPWFRDRVIPLAINNVSAFSKTQPGGYADNHP
                     ELEQFSPHDDRRPEAVAAALTAQGLQPVWKDWDSYLGRASQRL"
     gene            complement(14968..15738)
                     /gene="thiG"
                     /locus_tag="ACHOSC_RS04570"
                     /old_locus_tag="pgaptmp_000914"
     CDS             complement(14968..15738)
                     /gene="thiG"
                     /locus_tag="ACHOSC_RS04570"
                     /old_locus_tag="pgaptmp_000914"
                     /EC_number="2.8.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418418.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiazole synthase"
                     /protein_id="HGU4546902.1"
                     /translation="MLRIADKTFDSHLFTGTGKFASSQLMVEAIRASGSQLVTLAMKR
                     VDLRQHNDAILEPLIAAGVTLLPNTSGAKTAEEAIFAAHLAREALGTNWLKLEIHPDA
                     RWLLPDPIETLKAAETLVQQGFVVLPYCGADPVLCKRLEEVGCAAVMPLGAPIGSNQG
                     LETRAMLEIIIQQATVPVVVDAGIGVPSHAAQALEMGADAVLVNTAIAVADDPVNMAK
                     AFRLAVEAGLLARQSGPGSRSHFAHATSPLTGFLEASA"
     gene            complement(15740..15940)
                     /gene="thiS"
                     /locus_tag="ACHOSC_RS04575"
                     /old_locus_tag="pgaptmp_000915"
     CDS             complement(15740..15940)
                     /gene="thiS"
                     /locus_tag="ACHOSC_RS04575"
                     /old_locus_tag="pgaptmp_000915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfur carrier protein ThiS"
                     /protein_id="HGU4546903.1"
                     /translation="MQILFNDQPMQCAAGQTVHELLEQLDQRQAGAALAINQQIVPRE
                     QWAQHIVQDGDQILLFQVIAGG"
     gene            complement(15924..16679)
                     /gene="thiF"
                     /locus_tag="ACHOSC_RS04580"
                     /old_locus_tag="pgaptmp_000916"
     CDS             complement(15924..16679)
                     /gene="thiF"
                     /locus_tag="ACHOSC_RS04580"
                     /old_locus_tag="pgaptmp_000916"
                     /EC_number="2.7.7.73"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418420.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiazole biosynthesis adenylyltransferase ThiF"
                     /protein_id="HGU4546904.1"
                     /translation="MNDRDFMRYSRQILLDDIALDGQQKLLDSQVLIIGLGGLGTPAA
                     LYLAGAGVGTLVLADDDDVHLSNLQRQILFTTEDIDRPKSQVSQQRLTQLNPDIQLTA
                     LQQRLTGEALKDAVARADVVLDCTDNMATRQEINAACVALNTPLITASAVGFGGQLMV
                     LTPPWEQGCYRCLWPDNQEPERNCRTAGVVGPVVGVMGTLQALEAIKLLSGIETPAGE
                     LRLFDGKSSQWRSLALRRASGCPVCGGSNADPV"
     gene            complement(16672..17307)
                     /gene="thiE"
                     /locus_tag="ACHOSC_RS04585"
                     /old_locus_tag="pgaptmp_000917"
     CDS             complement(16672..17307)
                     /gene="thiE"
                     /locus_tag="ACHOSC_RS04585"
                     /old_locus_tag="pgaptmp_000917"
                     /EC_number="2.5.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709787.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004789 - thiamine-phosphate
                     diphosphorylase activity [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine phosphate synthase"
                     /protein_id="HGU4546905.1"
                     /translation="MYQPDFPPVPFRLGLYPVVDSVQWIERLLDAGVRTLQLRIKDRR
                     DEEVEADVVAAIALGRRYNARLFINDYWRLAIKHQAYGVHLGQEDLQATDLNAIRAAG
                     LRLGVSTHDDMEIDVALAARPSYIALGHVFPTQTKQMPSAPQGLEQLARHVERLADYP
                     TVAIGGISLARAPAVIATGVGSIAVVSAITQAADWRLATAQLLEIAGVGDE"
     gene            complement(17307..19202)
                     /gene="thiC"
                     /locus_tag="ACHOSC_RS04590"
                     /old_locus_tag="pgaptmp_000918"
     CDS             complement(17307..19202)
                     /gene="thiC"
                     /locus_tag="ACHOSC_RS04590"
                     /old_locus_tag="pgaptmp_000918"
                     /EC_number="4.1.99.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomethylpyrimidine synthase ThiC"
                     /protein_id="HGU4546906.1"
                     /translation="MSATKLTRREQRARAQHFIDTLEGTAFPNSKRIYITGTHPGVRV
                     PMREIQLSPTLIGGSKEQPQYEENEAIPVYDTSGPYGDPQIAINVQQGLAKLRQPWID
                     ARGDTEELTVRSSDYTKARLADDGLDELRFSGVLTPKRAKAGRRVTQLHYARQGIITP
                     EMEFIAIRENMGRERIRSEVLRHQHPGMSFGARLPENITAEFVRDEVAAGRAIIPANI
                     NHPESEPMIIGRNFLVKVNANIGNSAVTSSIEEEVEKLVWSTRWGADTVMDLSTGRYI
                     HETREWILRNSPVPIGTVPIYQALEKVNGIAEDLTWEAFRDTLLEQAEQGVDYFTIHA
                     GVLLRYVPMTAKRLTGIVSRGGSIMAKWCLSHHQENFLYQHFREICEICAAYDVSLSL
                     GDGLRPGSIQDANDEAQFAELHTLGELTKIAWEYDVQVMIEGPGHVPMQMIRRNMTEE
                     LEHCHEAPFYTLGPLTTDIAPGYDHFTSGIGAAMIGWFGCAMLCYVTPKEHLGLPNKE
                     DVKQGLITYKIAAHAADLAKGHPGAQIRDNAMSKARFEFRWEDQFNLALDPFTARAYH
                     DETLPQESGKVAHFCSMCGPKFCSMKISQEVRDYAATQTIEMGMADMSENFRARGGEI
                     YLRKEEA"
     regulatory      complement(19283..19383)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            complement(19435..19911)
                     /gene="rsd"
                     /locus_tag="ACHOSC_RS04595"
                     /old_locus_tag="pgaptmp_000919"
     CDS             complement(19435..19911)
                     /gene="rsd"
                     /locus_tag="ACHOSC_RS04595"
                     /old_locus_tag="pgaptmp_000919"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma D regulator"
                     /protein_id="HGU4546907.1"
                     /translation="MLNQLDNLTERVRGSNKLVDRWLHVRKHLLVAYYNLVGIKPGKE
                     SYMRLNEKALDDFCQSLVDYLSAGHFSIYERILHKLEGNGQLARAAKIWPQLEANTQQ
                     IMDYYDSSLETAIDHDNYLEFQQVLSDIGEALEARFVLEDKLILLVLDAARVKHPA"
     gene            20006..20779
                     /gene="nudC"
                     /locus_tag="ACHOSC_RS04600"
                     /old_locus_tag="pgaptmp_000920"
     CDS             20006..20779
                     /gene="nudC"
                     /locus_tag="ACHOSC_RS04600"
                     /old_locus_tag="pgaptmp_000920"
                     /EC_number="3.6.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000210 - NAD+ diphosphatase activity
                     [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(+) diphosphatase"
                     /protein_id="HGU4546908.1"
                     /translation="MDRIIEKLDHGWWVVSHEQKLWLPKGELPYGEAANFDLVGQRAL
                     QIGEWQGEPVWLVQQQRRHDMGSVRQVIDLDVGLFQLAGRGVQLAEFYRSHKYCGYCG
                     HEMYPSKTEWAMLCSHCRERYYPQIAPCIIVAIRRDDSILLAQHTRHRNGVHTVLAGF
                     VEVGETLEQAVAREVMEESGIKVKNLRYVTSQPWPFPQSLMTAFMAEYDSGDIVIDPK
                     ELLEANWYRYDDLPLLPPPGTVARRLIEDTVAMCRAEYE"
     gene            20819..21883
                     /gene="hemE"
                     /locus_tag="ACHOSC_RS04605"
                     /old_locus_tag="pgaptmp_000921"
     CDS             20819..21883
                     /gene="hemE"
                     /locus_tag="ACHOSC_RS04605"
                     /old_locus_tag="pgaptmp_000921"
                     /EC_number="4.1.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004853 - uroporphyrinogen decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen decarboxylase"
                     /protein_id="HGU4546909.1"
                     /translation="MTELKNDRYLRALLRQPVDVTPVWMMRQAGRYLPEYKATRAQAG
                     DFMSLCKNAELACEVTLQPLRRYPLDAAILFSDILTVPDAMGLGLYFEAGEGPRFTSP
                     VTCKADVDKLPIPDPEDELGYVMNAVRTIRRELKGEVPLIGFSGSPWTLATYMVEGGS
                     SKAFTVIKKMMYADPQALHALLDKLAKSVTLYLNAQIKAGAQAVMIFDTWGGVLTGRD
                     YQQFSLYYMHKIVDGLLRENDGRRVPVTLFTKGGGQWLEAMAETGCDALGLDWTTDIA
                     DARRRVGNKVALQGNMDPSMLYAPPARIEEEVATILAGFGHGEGHVFNLGHGIHQDVP
                     PEHAGVFVEAVHRLSEQYHR"
     gene            21893..22564
                     /gene="nfi"
                     /locus_tag="ACHOSC_RS04610"
                     /old_locus_tag="pgaptmp_000922"
     CDS             21893..22564
                     /gene="nfi"
                     /locus_tag="ACHOSC_RS04610"
                     /old_locus_tag="pgaptmp_000922"
                     /EC_number="3.1.21.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312948.1"
                     /note="cleaves DNA at apurinic or apyrimidinic sites;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribonuclease V"
                     /protein_id="HGU4546910.1"
                     /translation="MDLASLRAQQIELASSVIREDRLDKDPPDLIAGADVGFEQGGEV
                     TRAAMVLLKYPSLELVEYKVARIATTMPYIPGFLSFREYPALLAAWEMLSQKPDLVFV
                     DGHGISHPRRLGVASHFGLLVDVPTIGVAKKRLCGKFEPLSSEPGALAPLMDKGEQLA
                     WVWRSKARCNPLFIATGHRVSVDSALAWVQRCMKGYRLPEPTRWADAVASERPAFVRY
                     TANQP"
     gene            22607..23197
                     /gene="yjaG"
                     /locus_tag="ACHOSC_RS04615"
                     /old_locus_tag="pgaptmp_000923"
     CDS             22607..23197
                     /gene="yjaG"
                     /locus_tag="ACHOSC_RS04615"
                     /old_locus_tag="pgaptmp_000923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjaG family protein"
                     /protein_id="HGU4546911.1"
                     /translation="MLQNPIHLRLERLESWQHVTFMACLCERMYPNYAMFCQQTGFGD
                     GQIYRRILDLIWETLTVKDAKVNFDSQLEKFEEAIPSADDFDLYGVYPAIDACVALSE
                     LVHSRLSGETLEHAVEVSKTSITTVAMLEMTQAGREMSDEELKENPAVEQEWDIQWEI
                     FRLLAECEERDIELIKGLRADLREAGDSNIGIIFQQ"
     gene            23384..23656
                     /gene="hupA"
                     /locus_tag="ACHOSC_RS04620"
                     /old_locus_tag="pgaptmp_000924"
     CDS             23384..23656
                     /gene="hupA"
                     /locus_tag="ACHOSC_RS04620"
                     /old_locus_tag="pgaptmp_000924"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017460010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoid-associated protein HU-alpha"
                     /protein_id="HGU4546912.1"
                     /translation="MNKTQLIDVIAEKAELSKTQAKAALESTLAAITESLKEGDAVQL
                     VGFGTFKVNHRAERTGRNPQTGKEIKIAAANVPAFVSGKALKDAVK"
     gene            23669..24364
                     /gene="yjaH"
                     /locus_tag="ACHOSC_RS04625"
                     /old_locus_tag="pgaptmp_000925"
     CDS             23669..24364
                     /gene="yjaH"
                     /locus_tag="ACHOSC_RS04625"
                     /old_locus_tag="pgaptmp_000925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709795.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1481 domain-containing protein"
                     /protein_id="HGU4546913.1"
                     /translation="MNSFNEGVVSPLLSFWRRSLMLAGALFLTACSHNSSLPPFTASG
                     FAEDQGAVRIWRKDSGDNVHLLAVFSPWRSGDTTTREYRWQGDNLTLININVYSKPPV
                     NIRARFDDRGDLSFMQRESDGEKQQLSNDQIDLYRYRAAQIRQISDALRQGRVVLRQG
                     RWHAMEQTVTTCEGQTIKPDLDSQAIAHIERRQSRSSVDVSVAWLEAPEGSQLLLVAN
                     SDFCRWQPNEKTF"
     gene            complement(24366..24791)
                     /gene="zraP"
                     /locus_tag="ACHOSC_RS04630"
                     /old_locus_tag="pgaptmp_000926"
     CDS             complement(24366..24791)
                     /gene="zraP"
                     /locus_tag="ACHOSC_RS04630"
                     /old_locus_tag="pgaptmp_000926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312952.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc resistance sensor/chaperone ZraP"
                     /protein_id="HGU4546914.1"
                     /translation="MKRNTKIALVMMALSAMAMGSTSAFAHGGHGMWQQNAAPLTSEQ
                     QTAWQKIHNDFYAQSSALQQQLVTKRYEYNALLAANPPDSSKINAVAKEMENLRQSLD
                     ELRVKRDIAMAEAGIPRGAGMGMGYGGCGGGGHMGMGHW"
     gene            25029..26405
                     /gene="zraS"
                     /locus_tag="ACHOSC_RS04635"
                     /old_locus_tag="pgaptmp_000927"
     CDS             25029..26405
                     /gene="zraS"
                     /locus_tag="ACHOSC_RS04635"
                     /old_locus_tag="pgaptmp_000927"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     ZraS"
                     /protein_id="HGU4546915.1"
                     /translation="MRFMQRSKDSLAKWLSAILPVVIVGLVGLFAVTVIRDYGRETAA
                     ARQTLLEKGSVLIRALESGSRVGMGMRMHHAQQQALLEEMAGQPGVRWFAVTDEQGTI
                     VMHSNSGMVGKQLYSPQEMQQLHPGDEEAWRRIDSADGEPVLEIYRQFQPMFAAGMHR
                     MRHMQQYAATPQAIFIAFDASNIVSAEDREQRNTLIILFALATVLLASVLSFFWYRRY
                     LRSRQLLQDEMKRKEKLVALGHLAAGVAHEIRNPLSSIKGLAKYFAERAPAGGEAHQL
                     AQVMAKEADRLNRVVSELLELVKPTHLALQAVDLNTLINHSLQLVGQDANSREIQLRF
                     TANDTLPEIQADPDRLTQVLLNLYLNAIQAIGQHGVISVTVSESGAGVKISVTDSGKG
                     IAADQLEAIFTPYFTTKAEGTGLGLAVVHNIVEQHGGTIQVASQEGKGSTFTLWLPVN
                     ITRKDPQG"
     gene            26402..27727
                     /gene="zraR"
                     /locus_tag="ACHOSC_RS04640"
                     /old_locus_tag="pgaptmp_000928"
     CDS             26402..27727
                     /gene="zraR"
                     /locus_tag="ACHOSC_RS04640"
                     /old_locus_tag="pgaptmp_000928"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54-dependent response regulator
                     transcription factor ZraR"
                     /protein_id="HGU4546916.1"
                     /translation="MTHDNIDILVVDDDISHCTILQALLRGWGYNVALANSGRQALEQ
                     VRERVFDLVLCDVRMAEMDGIATLKEIKALNPAIPVLIMTAYSSVETAVEALKTGALD
                     YLIKPLDFDTLQATLEKALAHTHSIDAETPAVTASQFGMVGKSPAMQHLLSEIALVAP
                     SEATVLIHGDSGTGKELVARAIHASSARSEKPLVTLNCVALNESLLESELFGHEKGAF
                     TGADKRREGRFVEADGGTLFLDEIGDISPMMQVRLLRAIQEREVQRVGSNQTISVDVR
                     LIAATHRDLAAEVNAGRFRQDLYYRLNVVAIEVPSLRQRREDIPLLASHFLQRFAERN
                     RKAVKGFTPQAMDLLIHYDWPGNIRELENAVERSVVLLTGEYISERELPLAIASTPIP
                     LGQSQDIQPLVEVEKEVILAALEKTCGNKTEAARQLGITRKTLLAKLSR"
     gene            complement(27724..29013)
                     /gene="purD"
                     /locus_tag="ACHOSC_RS04645"
                     /old_locus_tag="pgaptmp_000929"
     CDS             complement(27724..29013)
                     /gene="purD"
                     /locus_tag="ACHOSC_RS04645"
                     /old_locus_tag="pgaptmp_000929"
                     /EC_number="6.3.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004637 - phosphoribosylamine-glycine
                     ligase activity [Evidence IEA];
                     GO_process: GO:0009113 - purine nucleobase biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylamine--glycine ligase"
                     /protein_id="HGU4546917.1"
                     /translation="MKVLVIGNGGREHALAWKAAQSPLVETVFVAPGNAGTALEPALQ
                     NVAIGVTDIPALLDFAQNEKIDLTIVGPEAPLVKGVVDTFRAAGLKIFGPTAGAAQLE
                     GSKAFTKDFLARHKIPTAEYQNFTEVEPALAYLREKGAPIVIKADGLAAGKGVIVAMT
                     LEEAEAAVHDMLAGNAFGDAGHRIVIEEFLDGEEASFIVMVDGEHVLPMATSQDHKRV
                     GDKDTGPNTGGMGAYSPAPVVTDEVHQRTMERIIWPTVKGMAAEGNTYTGFLYAGLMI
                     DKQGNPKVIEFNCRFGDPETQPIMLRMKSDLVELCLAACEGKLDEKTSEWDERASLGV
                     VMAAGGYPGDYRTGDVIHGLPLEEVAGGKVFHAGTKLADNEQVVTSGGRVLCVTALGH
                     TVAEAQKRAYALMTDIHWDDCFCRKDIGWRAIEREQN"
     gene            complement(29025..30614)
                     /gene="purH"
                     /locus_tag="ACHOSC_RS04650"
                     /old_locus_tag="pgaptmp_000930"
     CDS             complement(29025..30614)
                     /gene="purH"
                     /locus_tag="ACHOSC_RS04650"
                     /old_locus_tag="pgaptmp_000930"
                     /EC_number="2.1.2.3"
                     /EC_number="3.5.4.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003937 - IMP cyclohydrolase activity
                     [Evidence IEA];
                     GO_function: GO:0004643 -
                     phosphoribosylaminoimidazolecarboxamide formyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     phosphoribosylaminoimidazolecarboxamide
                     formyltransferase/IMP cyclohydrolase"
                     /protein_id="HGU4546918.1"
                     /translation="MQQRRPVRRALLSVSDKAGIVEFAQALSARGVELLSTGGTARLL
                     AEKGLPVTEVSDYTGFPEMMDGRVKTLHPKVHGGILGRRGQDDAIMEEHQIQPIDMVV
                     VNLYPFAQTVAREGCSLEDAVENIDIGGPTMVRSAAKNHKDVAIVVKSSDYDAIIKEM
                     DDNEGSLTLATRFDLAIKAFEHTAAYDSMIANYFGSMVPAYHGESKEAAGRFPRTLNL
                     NFIKKQDMRYGENSHQQAAFYIEENVKEASVATATQVQGKALSYNNIADTDAALECVK
                     EFAEPACVIVKHANPCGVAIGNSILDAYDRAYKTDPTSAFGGIIAFNRELDAETAQAI
                     ISRQFVEVIIAPSASEEALKITAAKQNVRVLTCGQWGERVPGLDFKRVNGGLLVQDRD
                     LGMVGAEELRVVTQRQPTEQELRDALFCWKVAKFVKSNAIVYAKNNMTIGIGAGQMSR
                     VYSAKIAGIKAADEGLEVKGSSMASDAFFPFRDGIDAAAAAGVTCVIQPGGSIRDDEV
                     IAAADEHGIAMLFTDMRHFRH"
BASE COUNT         7329 a         8062 c         8503 g         7187 t
ORIGIN      
        1 cgtcatactc cgttcttcaa aggctaccgt ccgcagttct acttccgtac tactgacgtg
       61 actggtacca tcgaactgcc ggaaggcgta gagatggtaa tgccgggcga caacatcaaa
      121 atggttgtta ccctgatcca cccgatcgcg atggacgacg gtctgcgttt cgcaatccgt
      181 gaaggcggcc gtaccgttgg cgcgggcgtt gtagcaaaag ttctgagcta attgccgata
      241 acatttgacg caatgcgcac taaaagggca tcatttgatg ccctttttgc acgctttcgt
      301 accagaacct ggctcatcag tgattttctt tgtcataatc attgctgaga caggctctgt
      361 tgagggcgta taatccgaaa agctaatacg cgtttcgatt tggtttgcct cgcgatcgcg
      421 gggtgaaaat gtttgtagaa aacttctgac aggttggttt atgagtgcga ataccgaagc
      481 tcaaggaagc gggcgcggcc tggaagcgat gaagtgggtc gttgtggtgg cattgctcct
      541 ggtggcgatt gtcggcaact acctttatcg cgacattatg ctgccgctgc gtgcgctggc
      601 cgtagtaatt ctgattgctg cagcgggtgg tgtcgcgctg ttaacgacaa aaggtaaagc
      661 taccgttgct tttgcccgtg aagcgcgtac cgaagtccgt aaggtcattt ggccgactcg
      721 ccaggaaaca ttgcacacca cgctgattgt ggctgcggtt accgcagtaa tgtcactgat
      781 cctgtgggga ctggatggta ttctggttcg cctggtatcc tttatcactg gcctgaggtt
      841 ctgagatgtc tgaagctcct aaaaagcgct ggtacgtcgt tcaggcgttt tccggttttg
      901 aaggccgcgt agcaacgtcg ctgcgtgagc atatcaaatt acacaacatg gaagatttgt
      961 ttggtgaagt catggtacca accgaagaag tggttgaaat ccgtggcggt cagcgtcgca
     1021 aaagcgaacg taaattcttc cctggctacg tcctcgttca gatggtgatg aacgacgcga
     1081 gctggcacct ggtgcgcagc gtaccgcgtg tgatgggctt catcggcggt acttccgatc
     1141 gtcctgcgcc aatcagcgat aaagaagtcg atgcgattat gaaccgcctg cagcaggttg
     1201 gtgataagcc gcgtccgaaa acgctgtttg aaccgggtga aatggtccgt gttaatgatg
     1261 gtccgttcgc tgacttcaac ggtgttgttg aagaagtgga ttacgagaaa tctcgtctga
     1321 aagtgtctgt ttctatcttc ggtcgtgcga ccccggtaga gctggacttc agccaggttg
     1381 aaaaagccta acccagcgat caaaaaagcg gcgatttaat cgttgcacaa ggcgtgagat
     1441 tggaatacaa tttcgcgcct tttgttttta tgggccttgc ccgtaaaacg attttttata
     1501 tcacggggag cctctcagag gcgttattac ccaacttgag gaatttataa tggctaagaa
     1561 agtacaagcc tatgtcaagc tgcaggttgc agctggtatg gctaacccga gtccgccagt
     1621 aggtccggct ctgggtcagc agggcgtaaa catcatggaa ttctgcaaag cgttcaacgc
     1681 aaaaactgat tccatcgaaa aaggtctgcc gattccggta gtaatcaccg tttacgctga
     1741 ccgttctttc actttcgtta ccaagacccc gccggcagca gttctgctga aaaaagcggc
     1801 tggtatcaag tctggttccg gtaagccgaa caaagacaaa gtgggtaaaa tttcccgcgc
     1861 tcagctgcag gaaatcgcgc agaccaaagc tgccgacatg actggtgccg acattgaagc
     1921 gatgactcgc tccatcgaag gtactgcacg ttccatgggc ctggtagtgg aggactaaga
     1981 aatggctaaa ctgaccaagc gcatgcgtgt tatccgcgag aaagttgatg caaccaaaca
     2041 gtacgacatc aacgaagcta tcgcactgct gaaagagctg gcgactgcta aattcgtaga
     2101 aagcatggac gtagctgtta acctcggcat cgacgctcgt aaatctgacc agaacgtacg
     2161 tggtgcaact gtactgccgc acggtactgg ccgttccgtt cgcgtagccg tatttaccca
     2221 aggtgcaaac gctgaagctg ctaaagctgc aggcgcagaa ctggtaggta tggaagatct
     2281 ggctgaccag atcaagaaag gcgaaatgaa ctttgacgtt gttattgctt ctccggatgc
     2341 aatgcgcgtt gttggccagc tgggccaggt tctgggtccg cgcggcctga tgccaaaccc
     2401 gaaagtgggt actgtaacac cgaacgttgc tgaagcggtt aaaaacgcta aagctggcca
     2461 ggttcgttac cgtaacgaca aaaacggcat catccacacc accatcggta aagtggactt
     2521 tgacgctgac aaactgaaag aaaacctgga agctctgctg gttgcgctga aaaaagcaaa
     2581 accgactcag gcgaaaggcg tgtacatcaa gaaagttagc atctccacca ccatgggtgc
     2641 aggtgttgca gttgaccagg ctggcctgag cgcttctgta aactaatgcc tttacgtggg
     2701 cggtgatttt gtctacaatc ttacccccac gtataatgct taatgcagac gtatatccga
     2761 gatattcggg ttgtggcaag gcggcaactg agtgagtcgc caggagcata gctaactatg
     2821 tgactggtgc gaatgaagga agccaacgcc gccacaagtt gaatagcgac ggatagaaaa
     2881 gatttgttcg ttggagcctg gcctatccag gcctccgtcg aagaccgcag gagtttcgca
     2941 agaaacttaa tcccctgcgt agacggtgac agaacgctaa gattattctt ttatattctg
     3001 gcttgtttct gctcaccgta attaagacgc tctctccgtt tggaggagtg aagtgagttc
     3061 cagagatttt ctctggcaaa catccaggag caaagctaat ggctttaaat cttcaagaca
     3121 aacaagcgat tgttgctgaa gtcagcgaag tagccaaagg cgcgctgtct gcagtagttg
     3181 cggattcccg tggcgtaact gtagataaaa tgactgaact gcgtaaagca ggtcgcgaag
     3241 ctggcgtata catgcgtgtt gttcgtaaca ccctgctgcg ccgtgctgtt gaaggtactc
     3301 cgttcgagtg cctgaaagac gcgtttgttg gtccgaccct gattgcatac tctatggaac
     3361 acccgggcgc tgctgctcgt ctgttcaaag agttcgcgaa agcgaatgca aaatttgagg
     3421 tcaaagccgc tgcctttgaa ggtgagctga tcccggcgtc tcagatcgac cgcctggcaa
     3481 ctctgccgac ctacgaagaa gcaattgcac gcctgatggc aaccatgaaa gaagcttcgg
     3541 ctggcaaact ggttcgtact ctggctgctg tacgcgatgc gaaagaagct gcttaatcgc
     3601 agttatcttt ttaacgcatt cgcttacgta taaacttatt ctgatattca ggaacaattt
     3661 aaatgtctat cactaaagat caaatcattg aagcagttgc agctatgtct gtaatggacg
     3721 ttgtagaact gatctctgca atggaagaaa aattcggtgt ttccgctgct gctgctgtag
     3781 ctgtagctgc tggcccggtt gaagctgctg aagaaaaaac tgaattcgac gtaattctga
     3841 aagctgctgg cgctaacaaa gttgctgtta tcaaagcagt acgtggcgca actggcctgg
     3901 gtctgaaaga agctaaagac ctggtagaat ctgcaccggc tgctctgaaa gaaggcgtga
     3961 gcaaagacga cgcagaagca ctgaaaaaag ctctggaaga agctggcgct gaagttgaag
     4021 ttaaataagc caacccttcc ggttgcagcc tgagaaatca ggctgatggc tggtgacttt
     4081 ttagtcacca gcctttttgc gctgtaaggc gccagtagcg tttcacactg tttgactact
     4141 gctgtgcctt tcaatgcttg tttctatcga cgacttaata tactgcgaca ggacgtccgt
     4201 tctgtgtaaa tcgcaatgaa atggtttaag cgtgatagca acaggcattg cggaaagtgt
     4261 tccattttcc ggtcaacaaa atagtgttgc acaaactgtc cgctcaatgg acagatgggt
     4321 cgacttgtca gcgagctgag gaaccctatg gtttactcct ataccgagaa aaaacgtatt
     4381 cgtaaggatt ttggtaaacg tccacaagtt ctggatgtac cttatctcct ttctatccag
     4441 cttgactcgt ttcagaaatt tatcgagcaa gatcctgaag ggcagtatgg tctggaagct
     4501 gctttccgtt ccgtattccc gattcagagc tacagcggta attccgagct gcaatacgtc
     4561 agctaccgcc ttggcgaacc ggtgtttgac gtccaggaat gtcaaatccg tggcgtgacc
     4621 tattccgcac cgctgcgcgt taaactgcgt ctggtgatct atgagcgcga agcgccggaa
     4681 ggcaccgtaa aagacattaa agaacaagaa gtctacatgg gcgaaattcc gctcatgaca
     4741 gacaacggta cctttgttat caacggtact gagcgtgtta tcgtttccca gctgcaccgt
     4801 agtccgggcg tcttctttga ctccgacaaa ggtaaaaccc actcttcggg taaagtgctg
     4861 tataacgcgc gcatcatccc ttaccgtggt tcctggctgg acttcgaatt cgatccgaag
     4921 gacaacctgt tcgtacgtat cgaccgtcgc cgtaaactgc ctgcgaccat cattctgcgc
     4981 gccctgaact acaccacaga gcagatcctc gacctgttct ttgaaaaagt tatctttgaa
     5041 atccgtgata acaagctgca gatggaactg gtgccggaac gcctgcgtgg tgaaaccgca
     5101 tcttttgata tcgaagctaa cggtaaagtg tacgtagaaa aaggccgccg tatcactgcg
     5161 cgccacattc gccagctgga aaaagacgac gtcaaactga tcgaagtccc ggttgagtac
     5221 atcgcaggta aagtggttgc taaagactat attgatgagt ctaccggcga gctgatctgc
     5281 gcagcgaaca tggagctgag cctggatctg ctggctaagc tgagccagtc tggtcacaag
     5341 cgtatcgaaa cgctgttcac caacgatctg gatcacggcc catatatctc tgaaacctta
     5401 cgtgtcgacc caactaacga ccgtctgagc gcactggtag aaatctaccg catgatgcgc
     5461 cctggcgagc cgccgactcg tgaagcagct gaaagcctgt tcgagaacct gttcttctcc
     5521 gaagaccgtt atgacttgtc tgcggttggt cgtatgaagt tcaaccgttc tctgctgcgc
     5581 gaagaaatcg aaggttccgg tatcctgagc aaagacgaca tcattgatgt tatgaaaaag
     5641 ctcatcgata tccgtaacgg taaaggcgaa gtcgatgata tcgaccacct cggcaaccgt
     5701 cgtatccgtt ccgttggcga aatggcggaa aaccagttcc gcgttggcct ggtacgtgta
     5761 gagcgtgcgg tgaaagagcg tctgtctctg ggcgatctgg ataccctgat gcctcaggat
     5821 atgatcaacg ccaagccgat ttccgcagca gtgaaagagt tcttcggttc cagccagctg
     5881 tctcagttta tggaccagaa caacccgctg tctgagatta cgcacaaacg tcgtatctcc
     5941 gcactcggcc caggcggtct gacccgtgaa cgtgcaggct tcgaagttcg agacgtacac
     6001 ccgactcact acggtcgcgt atgtccaatc gaaacccctg aaggtccgaa catcggtctg
     6061 atcaactctc tgtccgtgta cgcacagact aacgaatacg gcttccttga gactccgtat
     6121 cgtaaagtga ccgacggtgt tgtaactgac gaaattcact acctgtctgc tatcgaagaa
     6181 ggcaactacg ttatcgccca ggcgaactcc aacctggatg aagaaggcca cttcgtagaa
     6241 gacctggtaa cttgccgtag caaaggcgaa tccagcttgt tcagccgcga ccaggttgac
     6301 tacatggacg tatccaccca gcaggtggta tccgtcggtg cgtccctgat cccgttcctg
     6361 gaacacgatg acgccaaccg tgcattgatg ggtgcgaaca tgcaacgtca ggccgttccg
     6421 actctgcgcg ctgataagcc gctggttggt actggtatgg aacgtgctgt tgccgttgac
     6481 tccggtgtaa ctgcggtagc taaacgtggt ggtgtcgttc agtacgtgga tgcttcccgt
     6541 atcgttatca aagttaacga agacgagatg tatccgggtg aagcaggtat cgacatctac
     6601 aacctgacca aatacacccg ttctaaccag aacacctgta tcaaccagat gccgtgtgtg
     6661 tctctgggtg aaccggttga acgtggcgac gtgctggcag acggtccgtc caccgacctc
     6721 ggtgaactgg cgcttggtca gaacatgcgc gtagcgttca tgccgtggaa tggttacaac
     6781 ttcgaagact ccatcctcgt atccgagcgt gttgttcagg aagaccgttt caccaccatc
     6841 cacattcagg aactggcgtg tgtgtcccgt gacaccaagc tggggccgga agagatcacc
     6901 gctgacatcc cgaacgtggg tgaagctgcg ctctccaaac tggatgaatc cggtatcgtt
     6961 tacattggtg cggaagtgac cggtggcgac attctggttg gtaaggtaac gccgaaaggt
     7021 gaaactcagc tgaccccaga agaaaaactg ctgcgtgcga tcttcggtga gaaagcctct
     7081 gacgttaaag actcttctct gcgcgtacca aacggtgtat ccggtacggt tatcgacgtt
     7141 caggtcttta ctcgcgatgg cgtagaaaaa gacaaacgtg cgctggaaat cgaagaaatg
     7201 cagctcaaac aggcgaagaa agacctgtct gaagaactgc agatcctcga agcgggtctg
     7261 ttcagccgta tccgtgctgt gctggtagcc ggtggcgttg aagctgagaa gctcgacaaa
     7321 ctgccgcgcg atcgctggct ggagctgggc ctgacagacg aagagaaaca aaatcagctg
     7381 gaacagctgg ctgagcagta tgacgaactg aaacacgagt tcgagaagaa actcgaagcg
     7441 aaacgccgca aaatcaccca gggcgacgat ctggcaccgg gcgtgctgaa gattgttaag
     7501 gtatatctgg cggttaaacg ccgtatccag cctggtgaca agatggcagg tcgtcacggt
     7561 aacaagggtg taatttctaa gatcaacccg atcgaagata tgccttacga tgaaaacggt
     7621 acgccggtag acatcgtact gaacccgctg ggcgtaccgt ctcgtatgaa catcggtcag
     7681 atcctcgaaa cccacctggg tatggctgcg aaaggtatcg gcgacaagat caacgccatg
     7741 ctgaaacagc agcaagaagt cgcgaaactg cgcgaattca tccagcgtgc gtacgatctg
     7801 ggcgctgacg ttcgtcagaa agttgacctg agtaccttca gcgatgaaga agttatgcgt
     7861 ctggctgaaa acctgcgcaa aggtatgcca atcgcaacgc cggtgttcga cggtgcgaaa
     7921 gaagcagaaa ttaaagagct gctgaaactt ggcgacctgc cgacttccgg tcagatccgc
     7981 ctgtacgatg gtcgcactgg tgaacagttc gagcgtccgg taaccgttgg ttacatgtac
     8041 atgctgaaac tgaaccacct ggtcgacgac aagatgcacg cgcgttccac cggttcttac
     8101 agcctggtta ctcagcagcc gctgggtggt aaggcacagt tcggtggtca gcgtttcggg
     8161 gagatggaag tgtgggcgct ggaagcatac ggcgcagcat acaccctgca ggaaatgctc
     8221 accgttaagt ctgatgacgt gaacggtcgt accaagatgt ataaaaacat cgtggacggc
     8281 aaccatcaga tggagccggg catgccagaa tccttcaacg tattgttgaa agagattcgt
     8341 tcgctgggta tcaacatcga actggaagac gagtaattct cgctcaaaca ggtcactgct
     8401 gtcggggtaa aacccggcag cggattgtgc taactccgac gggagcaaat ccgtgaaaga
     8461 tttattaaag tttctgaaag cgcagactaa aaccgaagag tttgatgcga tcaaaattgc
     8521 tctggcttcg ccagacatga tccgttcatg gtctttcggt gaagttaaaa agccggaaac
     8581 catcaactac cgtacgttca aaccagaacg tgacggcctt ttctgcgccc gtatctttgg
     8641 gccggtaaaa gattacgagt gcctgtgcgg taagtacaag cgcctgaaac accgtggcgt
     8701 catctgtgag aagtgcggcg ttgaagtgac ccagactaaa gtacgccgtg agcgtatggg
     8761 ccacatcgaa ctggcttccc cgactgcgca catctggttc ctgaaatcgc tgccgtcccg
     8821 tatcggtctg ctgctcgata tgccgctgcg cgatatcgaa cgcgtactgt actttgaatc
     8881 ctatgtggtt atcgaaggcg gtatgaccaa cctggaacgt cagcagatcc tgactgaaga
     8941 gcagtatctg gacgcgctgg aagagttcgg tgacgaattc gacgcgaaga tgggtgcgga
     9001 agcaatccag gctctgctga agagcatgga tctggagcaa gagtgcgaac agctgcgtga
     9061 agagctgaac gaaaccaact ccgaaaccaa gcgtaaaaag ctgaccaagc gtatcaaact
     9121 gctggaagcg ttcgttcagt ctggtaacaa accagagtgg atgatcctga ccgttctgcc
     9181 ggtactgccg ccagatctgc gtccgctggt tccgctggat ggtggtcgtt tcgcgacttc
     9241 tgacctgaac gatctgtatc gtcgcgtcat taaccgtaac aaccgtctga aacgtctgct
     9301 ggatctggct gcgccggaca tcatcgtacg taacgaaaaa cgtatgctgc aggaagcggt
     9361 agacgccctg ctggataacg gtcgtcgcgg tcgtgcgatc accggttcta acaagcgtcc
     9421 tctgaaatct ttggccgaca tgatcaaagg taaacagggt cgtttccgtc agaacctgct
     9481 cggtaagcgt gttgactact ccggtcgttc tgtaatcacc gtaggtccat acctgcgtct
     9541 gcatcagtgc ggtctgccga agaaaatggc gctggagctg ttcaaaccgt tcatctacgg
     9601 caagctggaa ctgcgtggtc ttgctaccac cattaaagct gcgaagaaaa tggttgagcg
     9661 cgaagaagct gtcgtttggg atatcctgga cgaagttatc cgcgaacacc cggtactgct
     9721 gaaccgtgca ccgactctgc accgtctggg tatccaggca tttgaaccgg tactgatcga
     9781 aggtaaagct atccagctgc acccgctggt ttgtgcggca tataacgccg acttcgatgg
     9841 tgaccagatg gctgttcacg taccgctgac gctggaagcc cagctggaag cgcgtgcgct
     9901 gatgatgtct accaacaaca tcctgtcccc ggcgaacggc gaaccaatca tcgttccgtc
     9961 tcaggacgtt gttctgggtc tgtactatat gacccgtgac tgtgttaacg ccaaaggcga
    10021 aggcatggtg ctgactggcc cgaaagaagc agaacgtctg tatcgctctg gtctggcttc
    10081 tctgcatgcg cgcgttaaag tgcgtatcac cgagtatgaa aaagatgcaa acggtgaatt
    10141 agtagcgaaa accagcctga aagacacgac tgttggccgt gccattctgt ggatgattgt
    10201 accgaaaggt ctgccttact ccatcgtcaa ccaggcgctg ggtaaaaaag caatctccaa
    10261 aatgctgaac acctgctacc gcattctcgg tctgaaaccg accgttattt ttgcggacca
    10321 gatcatgtac accggcttcg cctatgcagc gcgttctggt gcatctgttg gtatcgatga
    10381 catggtcatc ccggagaaga aacacgaaat catctccgag gcagaagcag aagttgctga
    10441 aattcaggag cagttccagt ctggtctggt aactgcgggc gaacgctaca acaaagttat
    10501 cgatatctgg gctgcggcga acgatcgtgt atccaaagcg atgatggata acctgcaaac
    10561 tgaaaccgtg attaaccgtg acggtcagga agagaagcag gtttccttca acagcatcta
    10621 catgatggcc gactccggtg cgcgtggttc tgcggcacag attcgtcagc ttgctggtat
    10681 gcgtggtctg atggcgaagc cggatggctc catcatcgaa acgccaatca ccgcgaactt
    10741 ccgtgaaggt ctgaacgtac tccagtactt catctccacc cacggtgctc gtaaaggtct
    10801 ggcggatacc gcactgaaaa ctgcgaactc cggttacctg actcgtcgtc tggttgacgt
    10861 ggcgcaggac ctggtggtta ccgaagacga ttgtggtacc catgaaggta tcatgatgac
    10921 tccggttatc gagggtggtg acgttaaaga gccgctgcgc gatcgcgtac tgggtcgtgt
    10981 aactgctgaa gacgttctga agccgggtac tgctgatatc ctcgttccgc gcaacacgct
    11041 gctgcacgaa cagtggtgtg acctgctgga agagaactct gtcgacgcgg taaaagtacg
    11101 ttctgttgta tcttgtgaca ccgactttgg tgtatgtgcg cactgctacg gtcgtgacct
    11161 ggcgcgtggc cacatcatca acaaaggtga agcaatcggt gttatcgcgg cacagtccat
    11221 cggtgaaccg ggtacacagc tgaccatgcg tacgttccac atcggtggtg cggcatctcg
    11281 tgcggctgct gaatccagca tccaagtgaa aaacaaaggt agcatcaagc tcagcaacgt
    11341 gaagtcggtt gtgaactcca gcggtaaact ggttatcact tcccgtaata ctgaactgaa
    11401 actgatcgac gaattcggtc gtaccaaaga aagctacaaa gtaccttacg gtgcggtact
    11461 ggcgaaaggc gatggcgaac aggttgctgg cggcgaaacc gttgcaaact gggacccgca
    11521 caccatgccg gttatcaccg aagtaagcgg ttttgtacgc tttactgaca tgatcgacgg
    11581 tcagaccatt acgcgtcaga cagacgaatt gaccggtctg tcttcgctgg tggttctgga
    11641 ttccgcagaa cgtaccgcag gtggtaaaga tctgcgtccg gcactgaaaa tcgttgatgc
    11701 tcagggtaac gacgttctga ttccgggtac cgatatgcct gcgcagtact tcctgccggg
    11761 taaagcgatt gttcagctgg aagatggcgt acagatcagc tctggtgaca ccctggcgcg
    11821 tattccgcag gaatccggcg gtaccaagga catcaccggt ggtctgccgc gcgttgcgga
    11881 cctgttcgaa gcacgtcgtc cgaaagagcc ggcaatcctg gctgaaatca gcggtatcgt
    11941 ttccttcggt aaagaaacca aaggtaaacg tcgtctggtt atcaccccgg tagacggtag
    12001 cgatccgtac gaagagatga ttccgaaatg gcgtcagctc aacgtgttcg aaggtgaacg
    12061 tgtagaacgt ggtgacgtaa tttccgacgg tccggaagcg ccgcacgaca ttctgcgtct
    12121 gcgtggtgtt catgctgtta ctcgttacat cgttaacgaa gtacaggacg tataccgtct
    12181 gcagggcgtt aagattaacg ataaacacat cgaagttatc gttcgtcaga tgctgcgtaa
    12241 agctaccatc gttaacgcgg gcagctccga cttcctggaa ggcgaacagg ttgaatactc
    12301 tcgcgtcaag atcgcaaacc gcgaactgga agcgaacggc aaagtgggtg caacttactc
    12361 ccgcgatctg ctgggtatca ccaaagcgtc tctggcaacc gagtccttca tctccgcggc
    12421 atcgttccag gagaccactc gcgtgctgac cgaagcagcc gttgcgggca aacgcgacga
    12481 actgcgcggc ctgaaagaga acgttatcgt gggtcgtctg atcccggcag gtaccggtta
    12541 cgcgtaccac caggatcgta tgcgtcgccg tgctgcgggt gaagctccgg ctgcaccgca
    12601 ggtgactgca gaagacgcat ctgccagcct ggcagaactg ctgaacgcag gtctgggcgg
    12661 ttctgataac gagtaatcgt taatccgcaa ataacgtaaa aacccgcttc ggcgggtttt
    12721 tttatggggg gagtttaggg aaagagcatt tgtcagaata tttaaggaat ttctgaatac
    12781 tcataatcaa tgtagagatg actaatatcc tgaaactgac tgaactaatt gagtcaaact
    12841 cggcaaggat tcgatactat tcctgtgtaa ctttcttaag gaacgagaat gaaacaggaa
    12901 gtggaaaagt ggcgaccttt tggacatccg gatggtgata ttcgtgattt atcatttctt
    12961 gatgctcatc aggctgtcta cgttcagcat catgagggca aagagccttt agagtatcgc
    13021 ttttgggtta cctactctct tcactgcttc acaaaagatt atgaacatca gacgaacgaa
    13081 gaaaaacaat cgttaatgta ccacgcgcct aaagaatctc gtcccttctg ccagcaccgt
    13141 tataacttag cgcgcacaca cttaaaaaga actattttgg cgctgccaga aagcaacgtt
    13201 attcatgccg ggtatggtag ctatgccgtg attgaggtgg acttagacgg aggagataag
    13261 gcattttact ttgttgcgtt cagggctttc agggaaaaga aaaaactccg tttgcatgta
    13321 actagcgctt atcccatttc tgaaaaacag aaaggtaaat cagtgaaatt tttcaccatt
    13381 gcctacaact tattgagaaa taagcagctt cctcagccct caaaataaca aaacccacct
    13441 taaggtgggt ttcgccagag aattatctct ggtattcaga acgccattac cggactttgc
    13501 cttgaccttg cgataatcgc aggttgcggg atgtctgaat ttcttcagtc tgctgcatcc
    13561 tggaagatga gaacatgtgt tcttattttc gtctctatca tagttgagta tttactctct
    13621 tacaatcaga tctctttcat tgctcaacag gcgatggctt cagactttgc attacggaat
    13681 ttttaagaaa ggcagggcga aacgaggaag aagcttttcg ccctggacaa cattcctgct
    13741 gacggcacta ccataaaaaa acacaacgct ttcaacaacc ttgcgttttt catcttccgt
    13801 agcggctcgg aataaattta cgttcttaca ttccgtctca tagtctttgc gaggcccgtc
    13861 ccagatagct gtcccagtct ttccataccg gctgcaaacc ctgagcggtt aacgcggcag
    13921 caaccgcttc cggtctgcga tcgtcgtgcg gtgagaactg ttccaactcg gggtgattat
    13981 cggcatagcc acctggctgc gttttcgaga aggcgctgac gttattgatc gccagcggaa
    14041 taacgcgatc gcgaaaccac ggtgattccc gcgtggagag tgacagttca atctccggtg
    14101 caagcagtcg gaaggcgcag atggtttgca ctaactggcg ttcatccata atcgacgcag
    14161 gctcaatgcc gccagtacac gggcgcaggc gcggaaagga gacagagtaa cggctttgcc
    14221 agtaatgctg ttgcagccat agcaaatgtt cggcaaccat atagcagtca acgcgccagt
    14281 tgtcggaaag gccaattagc gcgccgaggc ctatcttatc aatccccgca cgccccagcc
    14341 gatccggcgt ttccagccgc cagaagaagt cctgtttttt tcctttcaga tgatggcggg
    14401 cataagtcgc ctcgtgatat gtctcctgat aaaccatcac gccatccaga ccaagctgct
    14461 ttaactcggc gtattccgtc tccgccagcg gttgcacttc catttgtagt gaagagaact
    14521 gttcacgcag ggcggggaga tgacgacgaa agtaatccat ccccactttc gcctgatgtt
    14581 caccagtgac taatagcaga tgttcaaagc ccatctcccg tatagcggcg ctttccctgg
    14641 caatatccgc ttcatccagc gttttgcgct tgatgcgatt actcatggaa aatccgcagt
    14701 acgtgcagtc gttagcgcaa agattggaaa gataaagcgg gacgtagaaa ctaactgtgt
    14761 tgccaaatcg ctgacgggtc agacgctgcg cccgttgggc cagttgttcc agatagccac
    14821 tggcggctgg cgataacagc gccatcatat cgtcgcgggt gagttgcgag gcatttagcg
    14881 cccgttctac gtcagcagcc gttttgccgt tgatacgcag gcggatgtcg tcccagtcca
    14941 gttgtcgcca gcgatcgctg aaggttttca tgccgatgcc tccagaaatc cagtcagcgg
    15001 gctggtggca tgagcaaaat gactgcggct gcccggtccg gactgacgtg ccagcaggcc
    15061 tgcttctacc gccagacgaa atgccttcgc catgttgacg ggatcgtccg cgacggcaat
    15121 tgccgtattc actaacaccg cgtcggcccc catttccagc gcctgcgcgg catggctggg
    15181 aacgccgatg ccagcatcga caaccaccgg cactgtggcc tgctggataa taatctccag
    15241 catggcgcgg gtttccagtc cctgattcga gccaatcggc gcgccgagcg gcatcaccgc
    15301 tgcacagccg acttcttcca gacgtttaca caataccgga tcggccccgc agtaaggcag
    15361 cacgacaaat ccctgttgta ccagcgtttc ggcggctttc agggtttcga tgggatcggg
    15421 caacagccag cgggcgtcag ggtgaatctc taattttaac cagtttgtgc ctaacgcttc
    15481 acgagccaga tgggcggcga aaatggcttc ttccgctgtt ttcgccccgg atgtatttgg
    15541 cagcagggtc acacccgccg cgataagcgg ttcgaggata gcgtcgttgt gctggcgcaa
    15601 gtcgacacgt ttcatcgcca gtgtcaccag ctggctgccg gaagcgcgga tcgcctccac
    15661 catcagttgt gaagaagcga atttccctgt gccggtaaac agatgtgaat caaacgtttt
    15721 gtccgcaata cgtaacattt caaccccctg caataacctg aaaaagcagg atctggtcgc
    15781 catcctgcac gatatgttgc gcccactgct cacgcgggac gatttgctga ttaatcgcca
    15841 gagccgcgcc cgcttgtcgt tggtcgagtt gctccagtag ttcgtgaaca gtttgcccgg
    15901 cggcgcactg catcggttga tcgttaaaca ggatctgcat tgcttcctcc gcataccggg
    15961 caaccactgg cgcggcgcaa cgccaggctg cgccactggc tcgatttacc gtcgaacagt
    16021 cggagttctc ccgcaggtgt ctctataccg cttaataact taatggcttc cagtgcctgc
    16081 aaagtgccca taaccccgac caccgggcca accacgcccg ccgtgcggca gttgcgttct
    16141 ggctcctggt tatctggcca caggcagcgg taacacccct gctcccaggg cggcgtcagt
    16201 accatcaact gaccgccaaa tccgaccgcg ctggcggtga taagcggcgt gttgagtgcc
    16261 acgcaggcgg cattaatctc ctggcgagtc gccatattgt cggtacagtc gagcaccaca
    16321 tcggcccgtg caaccgcatc ttttaacgcc tcacccgtta accgttgttg taatgctgtc
    16381 agttgaatgt cgggattcaa ctgtgtcagt cgctgttggc tgacctgcga tttcgggcga
    16441 tcgatatctt cagtggtaaa gaggatttgt cgttgcagat tgcttaaatg cacatcgtcg
    16501 tcatctgcca gtaccagcgt cccgacgcca gcgcccgcca ggtacagcgc agcaggtgta
    16561 cccagcccgc ccagaccgat aatcagcacc tggctgtcga gcagtttttg ctgcccgtcc
    16621 agagcgatat cgtcgagcag gatttggcgg ctataacgca taaagtcacg gtcattcatc
    16681 gccaactcct gcaatttcca gcaactgtgc cgttgccaaa cgccagtctg cggcttgagt
    16741 aatggcgctg acgacggcga tactgccgac acccgttgct atcaccgcag gcgcgcgtgc
    16801 cagactgata ccgccaatcg ccacggtggg ataatccgcc agtcgctcaa catgccgtgc
    16861 cagctgttcc agcccctgcg gtgcagaagg catctgtttg gtttgcgtcg ggaacacatg
    16921 tcccagcgcg atataagagg ggcgtgctgc cagcgcgacg tcgatttcca tatcgtcatg
    16981 tgtcgaaacg cccagccgca ggcctgccgc gcggatggca ttgagatcgg tggcttgcaa
    17041 atcttcctgc cccaaatgga cgccatacgc ctgatgcttg atcgccagcc gccagtaatc
    17101 gttgataaac aatcgcgcgt tatagcggcg gcccagcgca attgccgcca cgacatcggc
    17161 ttccacctct tcatcgcgcc gatctttgat gcgtagctgg agagtacgta cgcctgcatc
    17221 caacagacgt tcgatccact gtacgctgtc caccaccggg tacagtccta aacgaaaagg
    17281 tacaggagga aaatcaggct gatacatcac gcttcctcct tacgcaggta gatttctccg
    17341 cctctggcac ggaagttctc cgacatatcc gccattccca tttcaatagt ttgcgtggcg
    17401 gcgtaatcac gcacttcctg gctgattttc atcgagcaaa atttcggtcc acacatggag
    17461 caaaaatggg cgactttgcc ggactcttgg ggcagggttt catcgtgata agcgcgggcg
    17521 gtaaacgggt cgagggccag attaaactgg tcttcccagc gaaattcgaa gcgggctttc
    17581 gacatggcgt tatcgcgaat ttgcgcgccc ggatgccctt tcgccaggtc agcggcgtgg
    17641 gcggcaatct tataggtgat aagcccctgc ttaacatctt ctttattagg cagacccaga
    17701 tgctcttttg gcgttacgta acagagcatc gcgcagccaa accagccaat catcgccgca
    17761 ccaatccccg acgtgaagtg gtcatagccc ggcgcaatat cggtagttag cggccccaga
    17821 gtgtaaaacg gcgcttcgtg gcagtgctct aactcctcgg tcatattgcg gcggatcatc
    17881 tgcatcggca cgtggcctgg gccttcaatc atcacctgca cgtcatattc ccaggcaatt
    17941 ttggtcagtt cgcccagcgt atgcagctcg gcaaactgcg cttcatcgtt ggcgtcctga
    18001 atagaaccgg ggcgcagacc gtcgcccagc gacagcgaaa cgtcataagc ggcacagatt
    18061 tcacaaattt cgcggaagtg ttgatagagg aaattttcct gatgatggga gaggcaccat
    18121 ttcgccataa tcgaaccgcc gcgagagacg ataccggtca ggcgtttcgc ggtcatcggc
    18181 acatagcgca gcagtacgcc cgcatggata gtgaagtaat ccacaccttg ctcggcctgt
    18241 tccagcagcg tgtcgcggaa cgcttcccag gtaagatctt cggcgatccc gttaaccttc
    18301 tccagcgcct ggtagatcgg cactgtaccg atcggcaccg ggctgttacg caaaatccac
    18361 tcgcgggttt cgtgaatata gcgaccggtg gagagatcca tcaccgtatc cgctccccag
    18421 cgcgtggacc ataccagctt ttccacttct tcttcgatgg aagaggtgac cgcagagttg
    18481 ccgatattgg cgttaacttt taccaggaaa ttgcgaccaa taatcatcgg ctccgattcc
    18541 ggatgattaa tgttggccgg gataatcgca cgtccggcag caacttcatc acggacaaat
    18601 tccgcagtga tattttccgg cagacgtgcg ccaaagctca ttcccggatg ctggtggcgt
    18661 aaaacttcgc tacggatgcg ctcgcggccc atattctcgc ggatggcgat gaattccatt
    18721 tccggtgtga tgatgccctg gcgggcgtag tgcagttggg tgacgcggcg tcctgctttg
    18781 gcgcgttttg gtgttagtac gccgctaaaa cgcagttcgt cgaggccatc atctgccagc
    18841 cgcgctttag tgtaatcgga actgcgcacg gtaagttctt cggtatcgcc gcgcgcatcg
    18901 atccacggct ggcgtagttt tgccagccct tgctgcacgt taatggcaat ctgcggatca
    18961 ccatacgggc cggaggtgtc gtagaccgga atcgcttcgt tttcttcgta ctgcggctgt
    19021 tctttgctac cgccaattag cgtcgggcta agctggatct cacgcatcgg cacgcgcacg
    19081 ccggggtgtg tgccagtgat ataaatgcgt tttgagttgg gaaaggcggt gccttccagg
    19141 gtgtcgataa aatgttgggc ccgggcgcgt tgttcgcggc gggtcagttt tgttgcagac
    19201 atagctcatt ccaaaaagtt aaggacgtgg cttgtcagac gacggatgaa gcaagagacg
    19261 atcgccgcag gggcgatgcg atagcagatt aactcttgtt cccttcgcag gtattagcct
    19321 gatcaggttc cgcggatccc gaataaacgg tctcagccag ttaaggcact ccgacaagaa
    19381 attagccccg caaatggggc attgaatgta aattacgcgt taacagcgca gaactcaagc
    19441 aggatgtttg acgcgggcgg cgtcaagcac cagcagaatc agcttatctt ccagcacaaa
    19501 gcgcgcttcc agcgcctcgc cgatgtcaga taaaacctgt tgaaactcaa ggtaattatc
    19561 atgatcgatg gcggtttcca gactggaatc gtagtaatcc ataatctgtt gagtattggc
    19621 ttcgagttgc ggccaaatct tcgcggcgcg cgcgagttgc ccgttgcctt ccagcttatg
    19681 aagaatgcgc tcataaatac tgaaatgtcc ggcagacaag taatcgacca ggctctgaca
    19741 aaaatcatca agggcttttt cgtttagcct catgtacgat tctttgccag gcttaatgcc
    19801 aaccagattg tagtaagcca cgagcagatg cttacgtaca tgtagccagc gatcaaccag
    19861 tttgttactt cctctgacgc gctccgtcag gttatcgagc tggttaagca tgattgactc
    19921 cgcaagtttg tattcaaaaa ctgctcagtg agaaatgtaa aaaccatgtt aaacatgcca
    19981 gtgatgcaaa ggtagtgcaa gagctatgga tcgtataatt gaaaaattag atcacggctg
    20041 gtgggtcgtc agccatgaac aaaaattatg gttgccgaag ggagaattgc catatggcga
    20101 agcggcaaat ttcgatcttg tgggtcagcg cgcactacaa atcggcgaat ggcaggggga
    20161 acctgtttgg ttagtacaac agcagcggcg tcacgatatg gggtcggtac gtcaggtcat
    20221 tgatctcgat gttgggctgt ttcaactggc cggacgaggc gtacaactgg cggagtttta
    20281 ccgatcgcat aaatactgtg gttactgcgg gcatgaaatg tatccgagca aaaccgaatg
    20341 ggcgatgctg tgcagccatt gccgtgagcg ttactacccg caaatcgccc cctgcattat
    20401 tgttgctatc cgtcgcgatg attcgatcct cctcgcccag catacccgcc atcgtaacgg
    20461 tgtccataca gtacttgccg gattcgtcga agtgggcgaa accctcgagc aggcagtcgc
    20521 gcgggaagtg atggaagaga gcggaattaa agttaaaaac ttgcgttacg tgacttctca
    20581 gccgtggccg tttcctcagt ctttaatgac cgcgtttatg gcggaatatg acagcggcga
    20641 catcgtgatc gacccgaaag aattgctcga ggcgaactgg tatcgctatg acgatttgcc
    20701 gttactcccg ccgcccggca ccgtagcgcg ccgtctgata gaagatacgg tggcgatgtg
    20761 tcgggcagag tatgagtgat gatacactga ccgcctgacg cactaaggaa cagccaaaat
    20821 gaccgaactt aaaaacgatc gttatctgcg ggcgctgctg cgccagcccg ttgatgtcac
    20881 tccagtatgg atgatgcgcc aggcgggtcg ctatctaccg gaatataaag ccacgcgcgc
    20941 ccaggcaggc gattttatgt cgctgtgcaa aaacgccgag ctggcgtgcg aagtgacttt
    21001 gcaaccgctg cgtcgctacc cgctggatgc ggcgatcctc ttttccgata tcctcaccgt
    21061 gccggacgcg atggggttag ggctctattt tgaagccgga gaaggtccgc gttttacctc
    21121 gccagtcacc tgcaaagctg acgtcgataa actgccaatt ccggacccgg aagatgaact
    21181 gggttacgtg atgaacgcgg tgcgtaccat tcgtcgcgaa ctgaaaggcg aagtgccgct
    21241 gattggtttt tccggcagcc cgtggacgct ggcgacctac atggtggaag gcggcagcag
    21301 caaagccttc accgtgatca aaaaaatgat gtatgccgat ccgcaggcgc tgcacgctct
    21361 gctcgataaa ctggcgaaaa gcgtcacttt gtatctgaat gcgcagatta aagccggtgc
    21421 tcaggctgtg atgattttcg acacctgggg cggcgtgctt accgggcgcg attatcaaca
    21481 gttctcgctc tattacatgc ataaaattgt tgatggttta ctgcgtgaaa acgacggtcg
    21541 ccgcgtgcca gtcacgctgt ttaccaaagg cggcggacag tggctggaag cgatggcaga
    21601 aaccggttgc gatgcgctgg gcctcgactg gacaacggat atcgccgatg cgcgccgccg
    21661 cgtgggcaat aaagtcgcgt tgcagggcaa tatggatccg tcgatgctgt acgcgccgcc
    21721 agcccgcatt gaagaagaag tagcgactat acttgcaggt ttcggtcacg gcgaaggtca
    21781 tgtctttaac cttggtcacg gcattcatca ggatgtgccg ccagaacatg ctggcgtgtt
    21841 cgtggaggca gtgcatcgac tgtctgaaca gtatcaccgc taaggagtga ttatggatct
    21901 cgcgtcatta cgcgctcaac aaatcgaact ggcttcttct gtgatccgcg aggatcgact
    21961 cgataaagat ccaccggatc tgatcgccgg agccgatgtc gggtttgagc agggcggaga
    22021 agtgacgcga gcggcgatgg tgctgctgaa atatccctcg cttgagctgg tggagtataa
    22081 agtggcccgc atcgccacca ccatgcctta cattccaggt tttctttcct tccgcgaata
    22141 tcctgcgctg ctggcagcgt gggagatgct gtcgcaaaag ccggatttag tgtttgtcga
    22201 tggtcatggg atctcgcatc cccgccgtct tggcgtcgcc agccattttg gcttattggt
    22261 ggatgtgccg accattggcg tggcgaaaaa acggctctgc ggtaaattcg aaccgctctc
    22321 cagcgaaccg ggcgcgctgg ccccactgat ggataaaggc gagcagctgg cctgggtctg
    22381 gcgcagcaaa gcgcgctgta acccgttgtt tatcgctacc ggccatcggg tcagcgtgga
    22441 cagcgcgctg gcgtgggtac aacgctgcat gaaaggctat cgtctgccgg agccaacgcg
    22501 ctgggcggac gcggtggcct cggaacgtcc ggcgttcgtg cgctatacag caaatcagcc
    22561 ctaattcagg taaactgcgg ataatttccg tatttgagaa ctcaacatgt tacaaaaccc
    22621 aattcatctg cgtctggagc gcctggaaag ctggcagcat gtcactttca tggcttgctt
    22681 atgcgaacgc atgtacccca attacgccat gttctgccag caaaccggtt ttggtgatgg
    22741 gcaaatttac cgtcgcattc tcgatctcat ctgggaaacg ctgaccgtta aagacgcaaa
    22801 agtaaatttc gacagccaac tggagaaatt tgaagaagcg attccttcag ccgacgattt
    22861 cgatctgtac ggcgtttatc cggcaatcga tgcctgcgtg gcgttaagtg aactggtcca
    22921 ttcgcgtttg agtggtgaaa cgctcgaaca cgcggtggaa gtgagtaaga cctccatcac
    22981 gaccgttgcg atgctggaaa tgactcaggc tggtcgcgaa atgagcgatg aagagcttaa
    23041 agaaaaccca gctgtagagc aagaatggga cattcagtgg gaaatattcc gacttttagc
    23101 tgagtgcgaa gaacgcgata tcgagctgat aaaaggcctt agggcagacc tgcgtgaggc
    23161 gggtgatagc aatattggta taatttttca gcaataagac cagaaaacgt gatttaacgc
    23221 ctgatttgtc gtacctggag tcttcccttt cgccccccgt ctggtctaca tttggggggc
    23281 gaaaaaaagt ggctatcggt gcgtgtatgc aggagagtgc tattctggca tttccgtcgc
    23341 actcgatgct tagcaagcga taaacacatt gtaaggataa cttatgaaca agactcaact
    23401 gattgatgta attgcagaga aagcagaact gtccaaaacc caggctaaag ctgctctgga
    23461 gtccactctg gctgcaatta ctgagtctct gaaagaaggc gatgctgtac aactggttgg
    23521 tttcggtacc ttcaaagtga accaccgcgc tgagcgtact ggccgcaacc cgcagaccgg
    23581 taaagaaatc aaaattgccg cagctaacgt accggcattt gtttctggca aggcactgaa
    23641 agacgcagtt aagtaagatt gcgtggcagt gaacagtttt aacgaagggg tggtttcacc
    23701 ccttttgtct ttctggcgtc gatcattgat gctggcaggt gcgctgtttc tcactgcctg
    23761 tagccacaac tcttcacttc ctccctttac cgccagtgga tttgctgaag accagggcgc
    23821 ggtacgcatc tggcgaaaag acagcggcga taatgtgcat ctgcttgccg tgtttagccc
    23881 gtggcgcagt ggcgatacca cgacgcgaga gtatcgctgg cagggcgata acctcacgct
    23941 catcaatatc aatgtttaca gcaaaccgcc ggtgaacatt cgcgcgcgtt ttgacgatcg
    24001 cggtgatctg agctttatgc aacgtgaatc cgatggggaa aagcagcagc tttctaacga
    24061 ccaaatcgat ttataccgtt atcgtgctgc tcagatccgc cagattagcg atgccttacg
    24121 tcaggggaga gtcgtgctgc gccaggggcg ctggcatgcg atggaacaga ccgtgaccac
    24181 ctgcgaaggg caaaccatta aacctgattt agattcgcag gcgatagcgc atatcgagcg
    24241 ccgccagagc cgctcttctg ttgatgtcag cgtggcatgg ctggaagcgc ccgaaggttc
    24301 gcaattactg ttagtggcaa actctgattt ctgtcgctgg caacccaacg agaaaacgtt
    24361 ctgatttacc agtggcccat acccatatga ccgccaccac cgcagccgcc gtagcccatg
    24421 cccattccgg ctccgcgcgg aatacccgct tcagccatcg cgatatcccg tttcacccgt
    24481 aactcatcta acgactgacg caaattctcc atctctttgg cgaccgcgtt aattttgctg
    24541 ctatccggtg ggttcgcggc taacagggca ttgtattcat aacgcttcgt caccagttgc
    24601 tgttgcagtg cgctgctttg agcgtaaaag tcattatgga ttttctgcca cgctgtctgt
    24661 tgttcgctgg tcaaaggcgc ggcattttgc tgccacatac cgtgtccgcc gtgagcaaat
    24721 gcagatgtcg atcccatcgc cattgctgaa agcgccatca ttaccagggc aattttcgtg
    24781 ttccgtttca tggttaatcc tccagtggtt gtcttacttc gggtattgca tcttccgtgc
    24841 caacgatgaa acgctgatat gacgggtaat ctggcatgat aaacgagtaa aaatgactcg
    24901 cctgctgcgg gtagcgagtc atttttactc attgaaactt aagcctttgt gttacagcgc
    24961 agggtaagcg ctgataaaag atggcatgat ttctgctgtc agaaagggat gagcaggcaa
    25021 agaagaagat gcgttttatg caacgttcta aagactcctt agctaaatgg ttaagcgcga
    25081 tcctccccgt ggtcattgtt gggctggtag ggttgtttgc ggtgacggtg attcgcgatt
    25141 atgggcgcga gactgccgcc gccagacaaa cgctgctgga aaaaggcagt gtacttattc
    25201 gcgctcttga atccggctcg cgcgtcggca tggggatgcg catgcatcat gcgcagcagc
    25261 aggccttact ggaagaaatg gccgggcagc ctggagtacg ttggtttgcg gtcacggatg
    25321 aacaaggaac aatcgtgatg catagcaact ccggcatggt gggaaaacag ctttattccc
    25381 cgcaggaaat gcagcagtta catccgggag atgaagaagc gtggcggcgg atcgatagcg
    25441 cagacggcga gcctgttctg gaaatttatc gccagtttca accgatgttt gctgctggaa
    25501 tgcaccggat gcgccatatg cagcagtatg ccgcgacacc acaagcaatt ttcatcgctt
    25561 tcgacgccag taatattgtg agtgccgaag atcgtgagca gagaaacacc ctgattatcc
    25621 tcttcgccct ggcgacggtc ttgctggcaa gcgtattgtc attcttctgg tatcgccgct
    25681 atctgcgctc gcgccagctt ctacaagatg aaatgaagcg caaagagaag ctagtggcgc
    25741 tggggcatct tgcggcaggc gttgcccacg aaatccgtaa cccactttcc tcgattaaag
    25801 gactggcgaa atactttgcc gagcgcgcgc ctgcaggggg agaagcgcat caactggcgc
    25861 aggtgatggc gaaagaggcc gaccgtttaa accgcgtggt aagcgagctg ctggaactgg
    25921 ttaagccaac gcatctggct ttgcaggcgg tggatctcaa cacgctgatt aaccactcat
    25981 tacagctggt aggccaggat gcaaacagcc gggagatcca gttacgcttt accgccaacg
    26041 acacattacc ggaaattcag gccgatccgg acaggctgac tcaggtcctg ttgaatctct
    26101 atctcaatgc tattcaggcg attggtcagc atggcgtgat tagcgtgacg gtcagcgaaa
    26161 gcggcgcggg cgtgaaaatc agcgttaccg acagcggtaa gggaattgcg gcagatcagc
    26221 ttgaagccat cttcactccg tacttcacca ccaaagccga aggcaccgga ttggggctgg
    26281 cggtcgtgca taatattgtt gaacaacacg gtggtacaat tcaggtcgca agccaggagg
    26341 gaaaaggctc aacgttcacc ctctggcttc cggtcaatat tacgcgtaag gacccacaag
    26401 gatgacgcac gataatatcg atattctggt ggtggatgat gacattagcc actgcactat
    26461 tttgcaggct ttactgcgcg gctggggcta taacgtcgcg ctggcgaaca gcgggcgaca
    26521 ggcgctggag caggtgcggg agcgggtttt tgatcttgtg ctttgcgatg tgcgaatggc
    26581 agagatggac ggcattgcca cgctaaaaga gatcaaagcg ttaaatccgg cgattccggt
    26641 actgattatg accgcatact ccagcgtcga gacggcggta gaggcactga aaaccggggc
    26701 gctggattat ctcatcaagc cgctggattt cgacaccctg caggcgacgc tggaaaaggc
    26761 gctcgcgcat acgcacagta ttgatgctga aacgcctgcg gtgactgcca gccagttcgg
    26821 tatggtcggt aaaagcccgg cgatgcaaca cctgctcagt gaaatcgccc tcgtcgcgcc
    26881 atcggaagcc acggtgctga tccacggcga ttccggcacc ggtaaagagc tggtcgccag
    26941 ggcgattcac gccagtagcg cacgtagcga aaaaccgctg gtaacgctca actgtgtggc
    27001 actcaacgaa tccttgctgg aatctgaatt gttcggtcac gaaaaagggg cgtttactgg
    27061 agccgataaa cgccgggaag ggcgctttgt tgaggcggac ggcggcacgc tgtttctcga
    27121 tgaaattggc gatatctcgc cgatgatgca ggtgcgtctg ctgcgtgcga ttcaggagcg
    27181 cgaagttcag cgtgtcggta gcaaccagac tatctcggtt gatgtccggc tgattgcggc
    27241 gacccatcgc gatcttgccg cagaggtgaa tgccgggcgt tttcgccagg atctctacta
    27301 tcgcctgaat gtggtggcga ttgaagtacc atcgctgcgg caacggcggg aggatatccc
    27361 tctgctggct agccattttc ttcagcgttt tgccgagcgt aatcgcaagg cggtaaaagg
    27421 ctttacgcca caggcgatgg atttgctgat ccactacgac tggccgggaa acattcgtga
    27481 actggaaaac gcggtggaaa ggtcggtggt gctgctgacc ggggaatata tttccgaacg
    27541 cgagctgccg ctggcgattg ccagtacgcc gatcccgctg gggcaaagtc aggatattca
    27601 gccgctggtg gaagtcgaaa aagaagtgat tctggcggca ctggagaaaa cgtgcggcaa
    27661 caaaaccgaa gccgcccgtc agttagggat cacgcgcaaa acgctattgg caaaactgtc
    27721 gcgttagttc tgctcgcgtt cgatagcgcg ccagccgata tctttccggc agaagcagtc
    27781 gtcccagtga atatcggtca ttaaggcata agcgcgtttc tgcgcttctg ccacggtatg
    27841 acccagcgcg gtgacgcaca gtacgcgccc gccgctggtc actacctgct cgttatccgc
    27901 cagtttcgtg cccgcgtgga acactttgcc gcctgccact tcttccagcg gcaggccgtg
    27961 gatcacgtca ccggtgcggt aatcacccgg atatccaccc gcagccatca ccacgccgag
    28021 agaagcgcgt tcatcccact ctgacgtttt ctcgtccagt ttgccttcac aggccgccag
    28081 gcagagctca accagatcgg acttcatgcg cagcataatc ggctgggttt ctggatcgcc
    28141 aaagcggcag ttaaattcga taaccttcgg attgccctgt ttgtcgatca tcaggcccgc
    28201 gtagagaaaa ccggtgtagg tgttgccttc cgccgccatg cctttcacgg ttggccagat
    28261 gatacgttcc atggtgcgct gatgaacttc atcggttact accggcgcgg gggagtaagc
    28321 gcccatcccg cccgtgtttg gtccggtatc tttatcgcct acgcgtttgt gatcctggct
    28381 ggtggccatc ggcagcacat gctcgccgtc taccatcacg ataaagctcg cttcttcgcc
    28441 atcgaggaac tcttcgataa cgatgcgatg acccgcgtcg ccaaaagcgt tgcccgccag
    28501 catatcgtga acagccgctt ccgcttcttc cagcgtcatc gccacgataa cgcctttccc
    28561 ggcagccaga ccgtccgctt taatgacgat tggcgcgcct ttctcacgca gatacgccag
    28621 cgcaggttct acctcggtga agttctggta ttccgccgta gggatcttat ggcgggccag
    28681 gaaatctttg gtaaacgctt ttgagccttc cagttgggcc gcacctgcgg ttgggccgaa
    28741 gattttcagc ccggcggcgc ggaaggtatc gaccacgcct ttcaccagcg gcgcttccgg
    28801 gccgacgatg gtcagatcaa tcttttcgtt ttgtgcgaaa tccaacagcg ccgggatatc
    28861 ggtcacgcca atagcaacgt tttgcagcgc gggttccagt gcagtgcctg cattacccgg
    28921 agcaacaaaa acagtctcaa ccagcggcga ctgggccgct ttccaggcca gcgcgtgctc
    28981 gcgcccgccg ttaccaataa ctaatacttt catctattgc tccattaatg gcggaagtgg
    29041 cgcatgtcgg tgaagagcat cgcaataccg tgctcgtcgg cggcggcaat cacttcgtca
    29101 tcacggatgg aaccgccagg ctggattacg caggtcacgc ccgcagcggc ggcggcatca
    29161 ataccgtcgc ggaacgggaa gaacgcgtca gaagccatcg aggaaccttt cacttccagg
    29221 ccttcatcgg cagctttaat accggcgatt ttcgcggagt acacgcggct catctggccc
    29281 gcgccaatac cgatggtcat attgtttttg gcatagacga tagcattgga tttcacgaac
    29341 ttcgccactt tccagcagaa cagcgcatcg cgcagttcct gttcggtcgg ctgacgttgg
    29401 gtgacgacgc gcagttcttc cgcaccgacc atccccaggt cacgatcctg aaccagcaga
    29461 ccgccgttca cgcgtttgaa atcaagaccc ggaacacgct cgccccactg accgcaggtc
    29521 agaacgcgta cgttctgctt ggcggcggtg attttcaggg cttcttcgct ggcggaaggc
    29581 gcaataatca cttcgacaaa ctgacgagaa atgatggcct gtgcggtttc cgcatctagc
    29641 tcgcggttaa aggcaatgat gccgccgaat gcggaggttg ggtcggtttt gtacgcgcga
    29701 tcgtaagcat caagaatgga attgccgata gctacaccgc aagggttggc gtgcttcaca
    29761 atcacacatg ccggctcggc gaactctttc acgcactcca gcgccgcatc ggtatcggcg
    29821 atgttgttat aagagagggc tttaccctga acctgggttg cggtagcaac ggaggcttct
    29881 ttcacattct cttctatata gaaggcagcc tgctggtggc tgttctcgcc gtaacgcata
    29941 tcctgcttct taatgaagtt caggttcagc gtgcgtggga agcgaccagc ggcttctttg
    30001 ctttcaccgt ggtacgccgg aaccatgctg ccgaagtagt tggcaatcat gctgtcgtag
    30061 gcggcagtgt gttcgaaggc tttgatggcg aggtcgaaac gggttgcaag cgtcagcgat
    30121 ccttcgttgt catccatctc tttaataatg gcgtcgtagt cgctgctctt caccacgatt
    30181 gcgacatctt tatggttctt agcggcggag cgcaccatgg tcgggccgcc gatatcgatg
    30241 ttctcaaccg catcttccag cgagcaacct tcacgggcca cggtctgggc gaacggatac
    30301 aggttaacaa ccaccatatc gataggctgg atctgatgtt cttccataat ggcatcgtcc
    30361 tggccgcgac ggcccagaat gccaccatgt actttcggat gcagggtctt cacgcgtcca
    30421 tccatcatct ccgggaaacc ggtgtaatcg gaaacttcgg ttaccggcag acctttttct
    30481 gctaacagac gggcagtgcc ccctgtagac agcagctcca caccgcgtgc ggaaagtgcc
    30541 tgggcgaatt cgacgatacc ggctttgtca gaaacactga gcagagcgcg gcggactgga
    30601 cgacgttgtt gcatggtaaa tcccctggat ttgactatta cagagagcgt tagctgaatt
    30661 tttcgcgaaa aactcagcta acgcccctaa cggggcatcc ttatttttcg cccacattgt
    30721 aacgaaaacg tttgcgcaac gctcgcgaat ttttctcttt caatggtgat cacaattttg
    30781 acagtggtta ccgtgggaaa aatacagaaa ttacattgat gattgtggat aactctgtgc
    30841 gtaaaaaggt ataaagcggg gttttgctgg ggaatgcagc agtcagtcat ttttctgcaa
    30901 tttttctatt gcggcctgcg gagaactccc tataatgcgc ctccatcgac acggcggatg
    30961 tgaatcactt cacacaaaca gccggttcgg ttgaagagaa aaatcctgaa attcagggtt
    31021 gactctgaaa gaggaaagcg taatatacgc cacctcgcga cagtgcgcta aagcgcgtcg
    31081 c
//
LOCUS       DAZKHF020000024        30490 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2201S31241
            SAMN43267800-rid25106413.denovo.024, whole genome shotgun sequence.
ACCESSION   DAZKHF020000024 DAZKHF020000000
VERSION     DAZKHF020000024.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267800
            Sequence Read Archive: SRR30307263
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 30490)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 30490)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 30490)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350581.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350581.2
            Genome Coverage       :: 151x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:21:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,652
            CDSs (total)                      :: 5,557
            Genes (coding)                    :: 5,320
            CDSs (with protein)               :: 5,320
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 237
            CDSs (without protein)            :: 237
            Pseudo Genes (ambiguous residues) :: 0 of 237
            Pseudo Genes (frameshifted)       :: 102 of 237
            Pseudo Genes (incomplete)         :: 130 of 237
            Pseudo Genes (internal stop)      :: 52 of 237
            Pseudo Genes (multiple problems)  :: 41 of 237
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..30490
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267800-rid25106413.denovo.024"
                     /serotype="O 26:H11"
                     /isolate="2201S31241"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-01"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            complement(280..1971)
                     /gene="fruA"
                     /locus_tag="ACHOSC_RS04655"
                     /old_locus_tag="pgaptmp_000931"
     CDS             complement(280..1971)
                     /gene="fruA"
                     /locus_tag="ACHOSC_RS04655"
                     /old_locus_tag="pgaptmp_000931"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_function: GO:0022877 -
                     protein-N(PI)-phosphohistidine-fructose phosphotransferase
                     system transporter activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIBC"
                     /protein_id="HGU4546919.1"
                     /translation="MKTLLIIDANLGQARAYMAKTLLGAAARKAKLEIIDNPNDAEMA
                     IVLGDSIPNDSALNGKNVWLGDISRAVAHPELFLSEAKGHAKPYTAPVAATAPVAASG
                     PKRVVAVTACPTGVAHTFMAAEAIETEAKKRGWWVKVETRGSVGAGNAITPEEVAAAD
                     LVIVAADIEVDLAKFAGKPMYRTSTGLALKKTAQELDKAVAEATPYEPAGKAQTATSE
                     GKKESAGAYRHLLTGVSYMLPMVVAGGLCIALSFAFGIEAFKEPGTLAAALMQIGGGS
                     AFALMVPVLAGYIAFSIADRPGLTPGLIGGMLAVSTGSGFIGGIIAGFLAGYIAKLIS
                     TQLKLPQSMEALKPILIIPLISSLVVGLAMIYLIGKPVAGILEGLTHWLQTMGTANAV
                     LLGAILGGMMCTDMGGPVNKAAYAFGVGLLSTQTYGPMAAIMAAGMVPPLAMGLATMV
                     ARRKFDKAQQEGGKAALVLGLCFISEGAIPFAARDPMRVLPCCIVGGALTGAISMAIG
                     AKLMAPHGGLFVLLIPGAITPVLGYLVAIIAGTLVAGLAYAFLKRPEVDAVAKAA"
     gene            complement(1988..2926)
                     /gene="fruK"
                     /locus_tag="ACHOSC_RS04660"
                     /old_locus_tag="pgaptmp_000932"
     CDS             complement(1988..2926)
                     /gene="fruK"
                     /locus_tag="ACHOSC_RS04660"
                     /old_locus_tag="pgaptmp_000932"
                     /EC_number="2.7.1.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008662 - 1-phosphofructokinase activity
                     [Evidence IEA];
                     GO_function: GO:0016773 - phosphotransferase activity,
                     alcohol group as acceptor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-phosphofructokinase"
                     /protein_id="HGU4546920.1"
                     /translation="MSRRVATITLNPAYDLVGFCPEIERGEVNLVKTTGLHAAGKGIN
                     VAKVLKDLGIDVTVGGFLGKDNQDGFQQLFSELGIANRFQVVQGRTRINVKLTEKDGE
                     VTDFNFSGFEVTPADWERFVTDSLSWLGQFDMVCVSGSLPSGVSPEAFTDWMTRLRSQ
                     CPCIIFDSSREALVAGLKAAPWLVKPNRRELEIWAGRKLPEMKDVIEAAHALREQGIA
                     HVVISLGAEGALWVNASGEWIAKPPSVDVVSTVGAGDSMVGGLIYGLLMRESSEHTLR
                     LATAVAALAVSQSNVGITDRPQLAAMMARVDLQPFN"
     gene            complement(2926..4056)
                     /gene="fruB"
                     /locus_tag="ACHOSC_RS04665"
                     /old_locus_tag="pgaptmp_000933"
     CDS             complement(2926..4056)
                     /gene="fruB"
                     /locus_tag="ACHOSC_RS04665"
                     /old_locus_tag="pgaptmp_000933"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fused PTS fructose transporter subunit IIA/HPr
                     protein"
                     /protein_id="HGU4546921.1"
                     /translation="MFQLSVQDIHPGEKAGDKEEAIRQLAAALVQAGNVAEGYVNGML
                     AREQQTSTFLGNGIAIPHGTTDTRDQVLKTGVQVFQFTEGVTWGDGQVAYVAIGIAAS
                     SDEHLGLLRQLTHVLSDDSVAEQLKSATTAEELRALLMGEKQSEQLKLDNEMLTLDIA
                     ASDLLTLQALNAARLKEARAVDATFVTKAINEQPLNLGQGIWLSDSAEGNLRSAIAVS
                     RAANAFDVDGETAAMLVSVAMNDDQPIAVLKRLADLLLDNKADRLLKADAATLLALLT
                     SDDAPTDDVLSAEFVVRNEHGLHARPGTMLVNTIKQFNSDITVTNLDGTGKPANGRSL
                     MKVVALGVKKGHRLRFTAQGADAEQALKAIGDAIAAGLGEGA"
     gene            4424..5605
                     /gene="setB"
                     /locus_tag="ACHOSC_RS04670"
                     /old_locus_tag="pgaptmp_000934"
     CDS             4424..5605
                     /gene="setB"
                     /locus_tag="ACHOSC_RS04670"
                     /old_locus_tag="pgaptmp_000934"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005351 - carbohydrate:proton symporter
                     activity [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar efflux transporter SetB"
                     /protein_id="HGU4546922.1"
                     /translation="MHNSPAVSSAKSFDLTSTAFLIVAFLTGIAGALQTPTLSIFLTD
                     EVHARPAMVGFFFTGSAVIGILVSQFLAGRSDKRGDRKSLIVFCCLLGVLACTLFAWN
                     RNYFVLLFVGVFLSSFGSTANPQMFALAREHADKTGREAVMFSSFLRAQVSLAWVIGP
                     PLAYALAMGFSFTVMYLSAAVAFIVCGVMVWLFLPSMQKELPLATGTVEAPRRNRRDT
                     LLLFVICTLMWGSNSLYIINMPLFIINELHLPEKLAGVMMGTAAGLEIPTMLIAGYFA
                     KRLGKRFLMRVAAVGGVCFYAGMLMAHSPVILLGLQLLNAIFIGILGGIGMLYFQDLM
                     PGQAGSATTLYTNTSRVGWIIAGSVAGIVAEIWNYHAVFWFAMVMIIATLFCLLRIKD
                     V"
     gene            complement(5602..5856)
                     /gene="yeiW"
                     /locus_tag="ACHOSC_RS04675"
                     /old_locus_tag="pgaptmp_000935"
     CDS             complement(5602..5856)
                     /gene="yeiW"
                     /locus_tag="ACHOSC_RS04675"
                     /old_locus_tag="pgaptmp_000935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YkgJ family cysteine cluster protein"
                     /protein_id="HGU4546923.1"
                     /translation="MECRPGCGACCTAPSISSPIPGMPDGKPANTPCIQLDEQQRCKI
                     FTSPLRPKVCAGLQASAEMCGNSRQQAMTWLIDLEMLTAP"
     gene            6011..6583
                     /gene="yeiP"
                     /locus_tag="ACHOSC_RS04680"
                     /old_locus_tag="pgaptmp_000936"
     CDS             6011..6583
                     /gene="yeiP"
                     /locus_tag="ACHOSC_RS04680"
                     /old_locus_tag="pgaptmp_000936"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007750416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA];
                     GO_process: GO:0043043 - peptide biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P-like protein YeiP"
                     /protein_id="HGU4546924.1"
                     /translation="MPRANEIKKGMVLNYNGKLLLVKDIDIQSPTARGAATLYKMRFS
                     DVRTGLKVEERFKGDDIVDTVTLTRRYVDFSYVDGNEYVFMDKEDYTPYTFTKDQIEE
                     ELLFMPEGGMPDMQVLTWDGQLLALELPQTVDLEIVETAPGIKGASASARNKPATLST
                     GLVIQVPEYLSPGEKIRIHIEERRYMGRAD"
     gene            6806..8272
                     /gene="yeiQ"
                     /locus_tag="ACHOSC_RS04685"
                     /old_locus_tag="pgaptmp_000937"
                     /pseudo
     CDS             6806..8272
                     /gene="yeiQ"
                     /locus_tag="ACHOSC_RS04685"
                     /old_locus_tag="pgaptmp_000937"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416677.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol dehydrogenase family protein"
     gene            8390..9376
                     /gene="yeiR"
                     /locus_tag="ACHOSC_RS04690"
                     /old_locus_tag="pgaptmp_000938"
     CDS             8390..9376
                     /gene="yeiR"
                     /locus_tag="ACHOSC_RS04690"
                     /old_locus_tag="pgaptmp_000938"
                     /EC_number="3.6.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding GTPase YeiR"
                     /protein_id="HGU4546925.1"
                     /translation="MTRTNLITGFLGSGKTTSILHLLAHKDPNEKWAVLVNEFGEVGI
                     DGALLADSGALLKEIPGGCMCCVNGLPMQVGLNTLLRQGKPDRLLIEPTGLGHPKQIL
                     DLLTAPVYEPWIDLRATLCILDPRLLLDEKSASNENFRDQLAAADIIVANKSDRATPE
                     SEQALQRWWQQNGGDRQLIHSEHGKVDGHLLDLPRRNLAELPASAAHSHQHVVKKGLA
                     ALSLPEHQRWRRSLNSGQGYQACGWIFDADTVFDTIGILEWARLAPVERVKGVLRIPE
                     GLVRINRQGDDLHIETQNVAPPDSRIELISSSEADWNALQSALLKLRLATTA"
     gene            9415..10128
                     /gene="lpxT"
                     /locus_tag="ACHOSC_RS04695"
                     /old_locus_tag="pgaptmp_000939"
     CDS             9415..10128
                     /gene="lpxT"
                     /locus_tag="ACHOSC_RS04695"
                     /old_locus_tag="pgaptmp_000939"
                     /EC_number="2.7.4.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311093.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Kdo(2)-lipid A phosphotransferase"
                     /protein_id="HGU4546926.1"
                     /translation="MIKNLPQIVLLNIVGLALFLSWYIPVNHGFWLPIDADIFYFFNQ
                     KLVESKAFLWLVALTNNRAFDGCSLLAMGMLMLSFWLKENAPGRRRIVIMGLVMLLTA
                     VVLNQLGQALIPVKRASPTLTFTDINRVSELLSVPTKDASRDSFPGDHGMMLLIFSAF
                     MWRYFGKVAGLIALIIFVVFAFPRVMIGAHWFTDIIVGSMTVILIGLPWVLLTPLSDR
                     LITFFDKSLPGKNKHFQNK"
     gene            10540..11106
                     /gene="mepS"
                     /locus_tag="ACHOSC_RS04700"
                     /old_locus_tag="pgaptmp_000940"
     CDS             10540..11106
                     /gene="mepS"
                     /locus_tag="ACHOSC_RS04700"
                     /old_locus_tag="pgaptmp_000940"
                     /EC_number="3.4.17.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional murein DD-endopeptidase/murein
                     LD-carboxypeptidase"
                     /protein_id="HGU4546927.1"
                     /translation="MVKSQPILRYILRGIPAIAVAVLLSACSANNTAKNMHPETRAVG
                     SETSSLQASQDEFENLVRNVDVKSRIMDQYADWKGVRYRLGGSTKKGIDCSGFVQRTF
                     REQFGLELPRSTYEQQEMGKSVSRSNLRTGDLVLFRAGSTGRHVGIYIGNNQFVHAST
                     SSGVIISSMNEPYWKKRYNEARRVLSRS"
     gene            11287..12843
                     /gene="pdeN"
                     /locus_tag="ACHOSC_RS04705"
                     /old_locus_tag="pgaptmp_000941"
     CDS             11287..12843
                     /gene="pdeN"
                     /locus_tag="ACHOSC_RS04705"
                     /old_locus_tag="pgaptmp_000941"
                     /EC_number="3.1.4.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic di-GMP phosphodiesterase"
                     /protein_id="HGU4546928.1"
                     /translation="MFIRAPNSGRKLLLTCIVAGVMIAILVSCLQFLVAWHKHEVKYD
                     TLIIDVQKYLDTYFADLKSTTDRLQPLTLDTCQQANPELTARAAFSMNVRTFVLVKDK
                     KTFCSSATGEMDIPLNELIPALDINKNVDMAILPGTPMVPNKPAIVIWYRNPLLKNSG
                     VFAALNLNLTPSLFYSSRQEDYDGVALIIGNTALSTFSSRLMNVNELTDMPVRETKIA
                     GIPLTVRLYADDWTWNDVWYAFLLGGMSGTVVGLLCYYLMSVRMRPGREIMTAIKREQ
                     FYVAYQPVVDTQALRVTGLEVLLRWRHPVAGEIPPDAFINFAESQKMIVPLTQHLFEL
                     IARDAAELEKVLPVGVKFGINIAPDHLHSESFKADIQKLLTSLPAHHFQIVLEITERD
                     MLKEQEATQLFAWLHSVGVEIAIDDFGTGHSALIYLERFTLDYLKIDRGFINAIGTET
                     ITSPVLDAVLTLAKRLNMLTVAEGVETPEQARWLSERGVNFMQGYWISRPLPLDDFVR
                     WLKKPYTPQW"
     gene            12925..14739
                     /gene="yejA"
                     /locus_tag="ACHOSC_RS04710"
                     /old_locus_tag="pgaptmp_000942"
     CDS             12925..14739
                     /gene="yejA"
                     /locus_tag="ACHOSC_RS04710"
                     /old_locus_tag="pgaptmp_000942"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416682.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HGU4546929.1"
                     /translation="MIVRILLLFIALFTFGVQAQAIKESYAFAVLGEPRYAFNFNHFD
                     YVNPAAPKGGQITLSALGTFDTFNRYALRGNPGARTEQLYDTLFTTSDDEPGSYYPLI
                     AESARYADDYSWVEVAINPRARFHDGSPITARDVEFTFQKFMTEGVPQFRLVYKGTTV
                     KAIAPLTVRIELAKPGKEDMLSLFSLPVFPEKYWKDHKLSDPLATPPLASGPYRITSW
                     KMGQNIVYSRVKDYWAANLPVNRGRWNFDTIRYDYYLDDNVAFEAFKAGAFDLRMEND
                     AKNWATRYTGKNFDKKYIIKDEQKNESAQDTRWLAFNIQRPVFSDRRVREAITLAFDF
                     EWMNKALFYNAWSRTNSYFQNTEYAARNYPDAAELVLLAPMKKDLPPEVFTQIYQPPV
                     SKGDGYDRDNLLKADKLLNEAGWVLKGQQRVNATTGQPLSFELLLPASSNSQWVLPFQ
                     HSLQRLGINMDIRKVDNSQITNRMRSRDYDMMPRVWRAMPWPSSDLQISWSSEYINST
                     YNAPGVQSPVIDSLINQIIAAQGNKEKLLPLGRALDRVLTWNYYMLPMWYMAEDRLAW
                     WDKFSQPAVRPIYSLGIDTWWYDVNKAAKLPSASKQGE"
     gene            14740..15834
                     /gene="yejB"
                     /locus_tag="ACHOSC_RS04715"
                     /old_locus_tag="pgaptmp_000943"
     CDS             14740..15834
                     /gene="yejB"
                     /locus_tag="ACHOSC_RS04715"
                     /old_locus_tag="pgaptmp_000943"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="microcin C ABC transporter permease YejB"
                     /protein_id="HGU4546930.1"
                     /translation="MGAYLIRRLLLVIPTLWAIITINFFIVQIAPGGPVDQAIAAIEF
                     GNAGVLPGAGGEGVRASHAQTGVGNISDSKYRGGRGLDPEVIAEITHRYGFDKPIHER
                     YFKMLWDYIRFDFGDSLFRSASVLTLIKDSLPVSITLGLWSTLIIYLVSIPLGIRKAV
                     YNGSRFDVWSSAFIIIGYAIPAFLFAILLIVFFAGGSYFDLFPLRGLVSANFDSLPWY
                     QKITDYLWHITLPVLATVIGGFAALTMLTKNSFLDEVRKQYVVTARAKGVSEKNILWK
                     HVFRNAMLLVIAGFPATFISMFFTGSLLIEVMFSLNGLGLLGYEATVSRDYPVMFGTL
                     YIFTLIGLLLNIVSDISYTLVDPRIDFEGR"
     gene            15834..16859
                     /gene="yejE"
                     /locus_tag="ACHOSC_RS04720"
                     /old_locus_tag="pgaptmp_000944"
     CDS             15834..16859
                     /gene="yejE"
                     /locus_tag="ACHOSC_RS04720"
                     /old_locus_tag="pgaptmp_000944"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HGU4546931.1"
                     /translation="MSRLSPVNQARWARFRHNRRGYWSLWIFLVLFGLSLCSELIAND
                     KPLLVRYDGSWYFPLLKNYSESDFGGPLASQADYQDPWLKQRLENNGWVLWAPIRFGA
                     TSINFATDKPFPSPPSRQNWLGTDANGGDVLARILYGTRISVLFGLMLTLCSSVMGVL
                     AGALQGYYGGKVDLWGQRFIEVWSGMPTLFLIILLSSVVQPNFWWLLAITVLFGWMSL
                     VGVVRAEFLRTRNFDYIRAAQALGVSDRSIILRHMLPNAMVATLTFLPFILCSSITTL
                     TSLDFLGFGLPLGSPSLGELLLQGKNNLQAPWLGITAFLSVAILLSLLIFIGEAVRDA
                     FDPNKAV"
     gene            16861..18450
                     /gene="yejF"
                     /locus_tag="ACHOSC_RS04725"
                     /old_locus_tag="pgaptmp_000945"
     CDS             16861..18450
                     /gene="yejF"
                     /locus_tag="ACHOSC_RS04725"
                     /old_locus_tag="pgaptmp_000945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="microcin C ABC transporter ATP-binding protein
                     YejF"
                     /protein_id="HGU4546932.1"
                     /translation="MTQTLLAIEDLSVGFRHQQTVRTVVNDVSLQIEAGETLALVGES
                     GSGKSVTALSILRLLPSPPVEYLSGDIRFRGESLLHASDQTLRGVRGNKIAMIFQEPM
                     VSLNPLHTLEKQLYEVLSLHRGMRREAARGEILNCLDRVGIRQAAKRLTDYPHQLSGG
                     ERQRVMIAMALLTRPELLIADEPTTALDVSVQAQILQLLRELQGELNMGMLFITHNLS
                     IVRKLAHRVAVMQNGRCVEQNNAATLFASPTHPYTQKLLNSEPSGDPVPLPEPASTLL
                     DVEQLQVAFPIRKGILKRIVDHNVVVKNISFTLRAGETLGLVGESGSGKSTTGLALLR
                     LINSQGSIIFDGQPLQNLNRRQLLPIRHRIQVVFQDPNSSLNPRLNVLQIIEEGLRVH
                     QPTLSAAQREQQVIAVMHEVGLDPETRHRYPAEFSGGQRQRIAIARALILKPSLIILD
                     EPTSSLDKTVQAQILTLLKSLQQKHQLAYLFISHDLHVVRALCHQVIVLRQGEVVEQG
                     PCARVFATPQQEYTRQLLALS"
     gene            complement(18454..18798)
                     /gene="yejG"
                     /locus_tag="ACHOSC_RS04730"
                     /old_locus_tag="pgaptmp_000946"
     CDS             complement(18454..18798)
                     /gene="yejG"
                     /locus_tag="ACHOSC_RS04730"
                     /old_locus_tag="pgaptmp_000946"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YejG family protein"
                     /protein_id="HGU4546933.1"
                     /translation="MTSLQLSIVHRLPQNYRWSAGFAGSKVEPIPQNGPCGDNSLVAL
                     KLLSPDGDNAWSVMYKLSQALSDIEVPCSVLECEGEPCLFVNRQDEFAATCRLKNFGV
                     AIAEPFSNYNPF"
     gene            complement(19131..20321)
                     /gene="bcr"
                     /locus_tag="ACHOSC_RS04735"
                     /old_locus_tag="pgaptmp_000947"
     CDS             complement(19131..20321)
                     /gene="bcr"
                     /locus_tag="ACHOSC_RS04735"
                     /old_locus_tag="pgaptmp_000947"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter Bcr"
                     /protein_id="HGU4546934.1"
                     /translation="MTTRQHSSFAIVFILGLLAMLMPLSIDMYLPALPVISAQFGVPA
                     GSTQMTLSTYILGFALGQLIYGPMADSFGRKPVVLGGTLVFAAAAVACALANTIDQLI
                     VMRFFHGLAAAAASVVINALMRDIYPKEEFSRMMSFVMLVTTIAPLMAPIVGGWVLVW
                     LSWHYIFWILALAAILASAMIFFLIKETLPPERRQPFHIRTTIGNFAALFRHKRVLSY
                     MLASGFSFAGMFSFLSAGPFVYIEINHVAPENFGYYFALNIVFLFVMTIFNSRFVRRI
                     GALNMFRSGLWIQFIMAAWMVISALLGLGFWSLVVGVAAFVGCVSMVSSNAMAVILDE
                     FPHMAGTASSLAGTFRFGIGAIVGALLSLATFNSAWPMIWSIAFCASSSILFCLYASR
                     PKKR"
     gene            complement(20349..21044)
                     /gene="rsuA"
                     /locus_tag="ACHOSC_RS04740"
                     /old_locus_tag="pgaptmp_000948"
     CDS             complement(20349..21044)
                     /gene="rsuA"
                     /locus_tag="ACHOSC_RS04740"
                     /old_locus_tag="pgaptmp_000948"
                     /EC_number="5.4.99.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA pseudouridine(516) synthase RsuA"
                     /protein_id="HGU4546935.1"
                     /translation="MRLDKFIAQQLGVSRAIAGREIRGNRVTVDGEIVRNAAFKLLPE
                     HDVAYDGNPLAQQHGPRYFMLNKPQGYVCSTDDPDHPTVLYFLDEPVAWKLHAAGRLD
                     IDTTGLVLMTDDGQWSHRITSPRHHCEKTYLVTLESPVADDTAEQFAKGVQLHNEKDL
                     TKPAVLEVITPTQVRLTISEGRYHQVKRMFAAVGNHVVELHRERIGGITLDADLAPGE
                     YRPLTEEEIASVV"
     gene            21193..22953
                     /gene="radD"
                     /locus_tag="ACHOSC_RS04745"
                     /old_locus_tag="pgaptmp_000949"
     CDS             21193..22953
                     /gene="radD"
                     /locus_tag="ACHOSC_RS04745"
                     /old_locus_tag="pgaptmp_000949"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HGU4546936.1"
                     /translation="MIFTLRPYQQEAVDATLNHFRRHKTPAVIVLPTGAGKSLVIAEL
                     ARLARGRVLVLAHVKELVAQNHAKYQALGLEADIFAAGLKRKESHGKVVFGSVQSVAR
                     NLDAFQGEFSLLIVDECHRIGDDEESQYQQILTHLTKVNPHLRLLGLTATPFRLGKGW
                     IYQFHYHGMVRGDEKALFRDCIYELPLRYMIKHGYLTPPERLDMPVVQYDFSRLQAQS
                     NGLFSEADLNRELKKQQRITPHIISQIMEFAEKRKGVMIFAATVEHAKEIVGLLPAED
                     AALITGDTPGAERDVLIEDFKAQRFRYLVNVAVLTTGFDAPHVDLIAILRPTESVSLY
                     QQIVGRGLRLAPGKTDCLILDYAGNPHDLYAPEVGTPKGKSDNVPVQVFCPACGFANT
                     FWGKTTADGTLIEHFGRRCQGWFEDDDGHREQCDFRFRFKNCPQCNAENDIAARRCRE
                     CDTVLVDPDDMLKAALRLKDALVLRCSGMSLQHGHDEKGEWLKITYYDEDGADVSERF
                     RLQTPAQRTAFEQLFIRPHTRTPGIPLRWITAADILAQQALLRHPDFVVARMKGQYWQ
                     VREKVFDYEGRFRRAHELRG"
     gene            23078..23362
                     /gene="rplY"
                     /locus_tag="ACHOSC_RS04750"
                     /old_locus_tag="pgaptmp_000950"
     CDS             23078..23362
                     /gene="rplY"
                     /locus_tag="ACHOSC_RS04750"
                     /old_locus_tag="pgaptmp_000950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000494192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L25"
                     /protein_id="HGU4546937.1"
                     /translation="MFTINAEVRKEQGKGASRRLRAANKFPAIIYGGKEAPLAIELDH
                     DKVMNMQAKAEFYSEVLTIVVDGKEIKVKAQDVQRHPYKPKLQHIDFVRA"
     gene            complement(23501..24508)
                     /gene="yejK"
                     /locus_tag="ACHOSC_RS04755"
                     /old_locus_tag="pgaptmp_000951"
     CDS             complement(23501..24508)
                     /gene="yejK"
                     /locus_tag="ACHOSC_RS04755"
                     /old_locus_tag="pgaptmp_000951"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009295 - nucleoid [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoid-associated protein YejK"
                     /protein_id="HGU4546938.1"
                     /translation="MSLDINQIALHQLIKRDEQNLELVLRDSLLEPTETVVEMVAELH
                     RVYSAKNKAYGLFSEESELAQTLRLQRQGEEDFLAFSRAATGRLRDELAKYPFADGGF
                     VLFCHYRYLAVEYLLVAVLSNLSSMRVNENLDINPTHYLDINHADIVARIDLTEWETN
                     PESTRYLTFLKGRVGRKVADFFMDFLGASEGLNAKAQNRGLLQAVDDFTAEAQLDKAE
                     RQNVRQQVYSYCNEQLQAGEEIELESLSKELAGVSEVSFTEFAAEKGYELEESFPADR
                     STLRQLTKFAGSGGGLTINFDAMLLGERIFWDPATDTLTIKGTPPNLRDQLQRRTSGG
                     N"
     gene            24690..24917
                     /gene="yejL"
                     /locus_tag="ACHOSC_RS04760"
                     /old_locus_tag="pgaptmp_000952"
     CDS             24690..24917
                     /gene="yejL"
                     /locus_tag="ACHOSC_RS04760"
                     /old_locus_tag="pgaptmp_000952"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YejL family protein"
                     /protein_id="HGU4546939.1"
                     /translation="MPQISRYSDEQVEQLLAELLNVLEKHKAPTDLSLMVLGNMVTNL
                     INTSIAPAQRQAIANSFARALQSSINEDKAH"
     gene            24937..26697
                     /gene="yejM"
                     /locus_tag="ACHOSC_RS04765"
                     /old_locus_tag="pgaptmp_000953"
     CDS             24937..26697
                     /gene="yejM"
                     /locus_tag="ACHOSC_RS04765"
                     /old_locus_tag="pgaptmp_000953"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS biosynthesis-modulating metalloenzyme YejM"
                     /protein_id="HGU4546940.1"
                     /translation="MVTHRQRYREKVSQMVSWGHWFALFNILLSLVIGSRYLFIADWP
                     TTLAGRIYSYVSIIGHFSFLVFATYLLILFPLTFIVGSQRLMRFLSVILATAGMTLLL
                     IDSEVFTRFHLHLNPIVWQLVINPDENEMARDWQLMFISVPVILLLELVFATWSWQKL
                     RSLTRRRRFARPLAAFLFIAFIASHVVYIWADANFYRPITMQRANLPLSYPMTARRFL
                     EKHGLLDAQEYQRRLIEQGNPDAVSVQYPLSELRYRDMGTGQNVLLITVDGLNYSRFE
                     KQMPALAGFAEQNISFTRHMSSGNTTDNGIFGLFYGISPSYMDGILSTRTPAALITAL
                     NQQGYQLGLFSSDGFTSPLYRQALLSDFSMPSVRTQSDEQTATQWINWLGRYAQEDNR
                     WFSWVSFNGTNIDDSNQQAFARKYSRAAGNVDDQINRVLNALRDSGKQDNTVVIITAG
                     RGIPLSEDEETFDWSHGHLQVPLVIHWPGTPAQRINALTDHTDLMTTLMQRLLHVSTP
                     ASEYSQGQDLFNPQRRHYWVTAADNDTLAITTPKKTLVLNNNGKYRTYNLRGERVKDE
                     KPQLSLLLQVLTDEKRFIAN"
     gene            26772..26848
                     /locus_tag="ACHOSC_RS04770"
                     /old_locus_tag="pgaptmp_000954"
     tRNA            26772..26848
                     /locus_tag="ACHOSC_RS04770"
                     /old_locus_tag="pgaptmp_000954"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:26806..26808,aa:Pro,seq:ggg)
     gene            27067..28308
                     /locus_tag="ACHOSC_RS04775"
                     /old_locus_tag="pgaptmp_000955"
     CDS             27067..28308
                     /locus_tag="ACHOSC_RS04775"
                     /old_locus_tag="pgaptmp_000955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000101915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="integrase domain-containing protein"
                     /protein_id="HGU4546941.1"
                     /translation="MARKTHPLTTVQIKAARPAQKEYTLQDGGGLFLLVKPSGSKLWR
                     FSYYRPSDKKRILLSFGSLEDVSLADARKRRSEYRTLISAGTDPQDHERQKRETEARR
                     QGNTFENVAAAWYQVKISQNLAPNTIKDIWRSLDKYVFPFIGNTPIDTLTARRFVEVL
                     TPIKERGNLETLKRVLQRVNEVMDYAANSGLIDANPAMNVRKAFPSPVKKHMPTIRPE
                     QLPELMQALSVSATERQTRLLIEWQLLTVTRPAEASSTRWDEINLDAKQWTIPAGRMK
                     MRRDHVIPLSGQAMAVLEAMKPISHHRNYVFPSLKDPQQPMNSQTANAALRRMGFAGV
                     LVSHGLRAIFSTAANEEGFEPDVIEAALAHVDTNEVRRAYNRSNYIEKRIVLMRWWGE
                     FVEAAATGVTLASGKRGIRAV"
     gene            28407..28637
                     /locus_tag="ACHOSC_RS04780"
                     /old_locus_tag="pgaptmp_000956"
     CDS             28407..28637
                     /locus_tag="ACHOSC_RS04780"
                     /old_locus_tag="pgaptmp_000956"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001171356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4546942.1"
                     /translation="MQKNIRCNCDGLQLALMVQHEFWSTYDPEDRTTAPSKKQVVDFL
                     VSRGASRNLAVSIDKVVRPASMKIGGRPKKWR"
     gene            28805..29011
                     /locus_tag="ACHOSC_RS04785"
                     /old_locus_tag="pgaptmp_000957"
     CDS             28805..29011
                     /locus_tag="ACHOSC_RS04785"
                     /old_locus_tag="pgaptmp_000957"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000387479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HGU4546943.1"
                     /translation="MEAIRKIIFRQEVKKIIHIKADSTLQSMINAGEFPQGFRVGLRR
                     RGWYEDDVLSWQKEREEEARGTAA"
     gene            29026..29502
                     /locus_tag="ACHOSC_RS04790"
                     /old_locus_tag="pgaptmp_000958"
                     /pseudo
     CDS             29026..29502
                     /locus_tag="ACHOSC_RS04790"
                     /old_locus_tag="pgaptmp_000958"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309603.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="host cell division inhibitor Icd-like protein"
     gene            29695..30033
                     /locus_tag="ACHOSC_RS04795"
                     /old_locus_tag="pgaptmp_000959"
                     /pseudo
     CDS             29695..30033
                     /locus_tag="ACHOSC_RS04795"
                     /old_locus_tag="pgaptmp_000959"
                     /inference="COORDINATES: protein motif:HMM:NF022029.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ash family protein"
     gene            30064..30255
                     /locus_tag="ACHOSC_RS04800"
                     /old_locus_tag="pgaptmp_000960"
     CDS             30064..30255
                     /locus_tag="ACHOSC_RS04800"
                     /old_locus_tag="pgaptmp_000960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="host cell division inhibitor Icd-like protein"
                     /protein_id="HGU4546944.1"
                     /translation="MATIPTLVHSQTAFLWRFIIFGASEHQIIHVTAWTEREARSRCP
                     SGCVAVFAARIRQEVCHVQ"
     gene            30245..30466
                     /locus_tag="ACHOSC_RS04805"
                     /old_locus_tag="pgaptmp_000961"
     CDS             30245..30466
                     /locus_tag="ACHOSC_RS04805"
                     /old_locus_tag="pgaptmp_000961"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001372127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4546945.1"
                     /translation="MCNKTTPDAAAAALTTLMHALIDIECTAELAQGEEQKDRTQFAL
                     ECIRYIATRSLNDAKNILVADCENGGGYA"
BASE COUNT         7433 a         7915 c         7621 g         7521 t
ORIGIN      
        1 aaagcgggca gatttgagca ggctcacaaa atgcgattaa atgccagaat tacaaaagcg
       61 aggaatttgt gacgggatgc acagaacagg aacactaaaa aagggcagaa aagaatctgc
      121 ccttgaggaa ataaccttag tgtttgatga acgtttcagg tttctactga aagataaaac
      181 catttaaatt cataaaatta cattcacaaa ccggtcattc tctttgcgct catccaatga
      241 tacagggcag taaatttctg ccctgtaaca caccttttat tacgcggctt tcgctactgc
      301 gtccacttcc ggacgtttca ggaaggcata ggccaaaccc gccaccagcg taccggcaat
      361 aattgctacc aggtaaccca gtaccggcgt aatagcgcca gggatcagca gaacaaacag
      421 accaccgtgc ggtgccatca gtttcgcacc aatcgccatt gagattgcgc cagtcagcgc
      481 cccaccaacg atacaacacg gcagcacacg catcggatca cgagcagcaa acggaattgc
      541 accttccgaa atgaagcaca gtcccaatac cagagcggct ttgccacctt cctgctgcgc
      601 tttgtcgaat ttgcgacgcg ccaccattgt tgccagaccc attgccagcg gtggcaccat
      661 acctgccgcc ataatcgccg ccatcgggcc ataggtttga gtactcagca gacccacacc
      721 gaatgcgtac gctgctttgt ttaccggacc gcccatgtca gtacacatca tgccaccgag
      781 gatcgccccc agcagaaccg cattcgcagt ccccatggtc tgcagccagt gagtcaaccc
      841 ttcgagaatg ccagcaactg gtttaccgat caggtagatc atcgccagac cgaccaccag
      901 actggaaatt agcggaatga tcaggatcgg tttcagcgcc tccatactct gtggcagttt
      961 cagttgcgta ctgattaact tcgcaatgta accagccagg aagcccgcaa taataccgcc
     1021 aatgaagcca gaaccggtgc tgaccgccag cataccgcca atcagacccg gcgtgagacc
     1081 cggacgatcg gcaatggaga aggcaatgta acctgccagt accggcacca tcagcgcaaa
     1141 ggctgaacca ccaccaatct gcatcagcgc cgctgccaac gtacccggct ctttaaacgc
     1201 ttcgatacca aaagcaaaag aaagcgcgat acacagacca cctgcaacga ccatcggcag
     1261 catgtaagag acgcccgtca gcaagtgacg gtatgcgcct gcactctctt tcttaccttc
     1321 agaggtcgcc gtttgagctt tgcccgccgg ttcatacggc gttgcttcag caaccgcttt
     1381 atccagttcc tgcgcggttt tcttcagcgc cagaccggta gaggtacgat acatcggttt
     1441 accagcaaat ttcgccagat ccacttcgat atctgccgcc acaatcacca gatccgctgc
     1501 ggctacttct tcgggggtga ttgcattacc cgcaccaaca gaaccacggg tttcaacttt
     1561 cacccaccag ccacgttttt tcgcttcggt ttcaatggct tcagccgcca taaaggtgtg
     1621 tgctacgcca gtcgggcaag cagtcaccgc aactacgcgt ttcggaccgc tggcggcaac
     1681 cggtgctgtc gcagcgaccg gcgcagtgta aggtttcgca tggcctttgg cttcactcag
     1741 gaacagctca gggtgcgcaa ctgcccggga aatatcaccc agccagacat ttttaccgtt
     1801 cagcgcgctg tcattcggga tggaatcacc gagaacaatc gccatttctg cgtcgttcgg
     1861 attgtcgatg atttccagtt ttgcttttcg cgccgccgcg cccagcaggg tcttcgccat
     1921 ataggcgcgt gcctgaccga gattagcgtc aataatcagc agcgttttca ttatgcctct
     1981 cctgctgtca gttaaaaggt tgtaagtcga cgcgcgccat cattgcggcc aactgcggac
     2041 gatcggtaat acccacattg ctttgactta ccgccagggc tgcaacagca gtcgccagac
     2101 gcagtgtgtg ttcactggat tcacgcatca gcaagccata aatcaggcca ccaaccatag
     2161 aatcccctgc gccaacggtg cttacgacat cgactgacgg tggtttggcg atccattcgc
     2221 cggaggcatt aacccaaagc gcgccttcgg cacccagtga aataacaaca tgcgcaatac
     2281 cttgttcacg tagcgcatgt gcagcttcaa tcacatcttt catttcaggc agtttacggc
     2341 ctgcccagat ttccaactcg cggcggttag gtttcaccag ccacggtgcc gctttcaaac
     2401 ctgctactaa cgcttcacgg ctactatcaa agataatgca aggacactga ctacgcaggc
     2461 gagtcatcca gtcggtgaac gcttccgggc tgacgcctga cggtaagctt ccgctgacac
     2521 agaccatatc gaactgaccg agccagctca gagaatcagt cacaaagcgt tcccagtcgg
     2581 cgggggtgac ttcaaaaccc gagaagttga agtcggtcac ttcgccgtct ttttccgtca
     2641 gcttaacgtt aattcgggtg cgcccctgta caacctggaa acggttggca atgcccagct
     2701 cgctgaacag ttgctgaaaa ccatcctgat tgtctttacc caggaagccg ccaacggtga
     2761 catcaattcc caggtctttt aatactttgg ccacgttgat gcctttaccc gccgcatgca
     2821 gaccggtggt tttcaccagg ttcacttcgc cgcgttcaat ttccgggcag aaaccaacaa
     2881 ggtcataagc cggattaagg gtgatagtag caacacgtct gctcattatg cgccctcccc
     2941 aagaccagca gcgatagcgt cgccgattgc tttcagcgcc tgttcagcat ctgcaccctg
     3001 ggcggtaaag cgtaggcgat gacctttctt aacgccaagt gccacaactt tcatcagact
     3061 acgtccgttt gccggtttgc cggtgccatc aaggtttgtc acggtaatat cactgttaaa
     3121 ttgtttaatg gtgttgacca gcatggtacc tggacgagca tgcaggccgt gttcattgcg
     3181 caccacaaac tccgcgctta acacgtcgtc ggtcggcgca tcatcgctgg tcagcagcgc
     3241 cagcaacgtt gccgcatccg ctttcagcaa gcggtcagct ttattgtcga gcaacaaatc
     3301 agcgagacgc ttaagaaccg caatgggctg atcgtcattc atcgccacac tcaccagcat
     3361 ggctgccgtt tcgccgtcca catcaaaagc atttgccgca cggcttaccg caatcgcgct
     3421 acgcagattg ccttcggcgc tatcgctcag ccagataccc tgtccgaggt tcagcggttg
     3481 ttcattgatg gctttggtga cgaaagtggc gtcaactgcc ctcgcctctt tcagacgcgc
     3541 agcgttcagc gcctgaagag tcagcagatc gctggcggcg atatccagcg tcagcatttc
     3601 gttgtcgagc ttcagctgct cactctgctt ttcgcccatc agcaatgcgc gaagttcttc
     3661 tgctgttgtt gctgacttca gttgttcagc aacggaatca tcgctcagta cgtgggtcag
     3721 ctggcgtagc aggcccagat gttcatccga gctggcagca ataccgatcg ctacgtacgc
     3781 tacctgaccg tcaccccagg tgacgccttc cgtgaactga aatacctgaa cgccggtttt
     3841 cagcacctga tcgcgggtgt cggtagtgcc gtgtggaata gcaataccat tgccgaggaa
     3901 cgttgaggtt tgctgttcgc gcgccagcat gccattgacg tagccttctg ctacgttacc
     3961 ggcctgcacc agcgccgcag cgagctggcg aatcgcctct tctttgtctc cggccttttc
     4021 gcccggatgg atgtcctgta cggataactg gaacatagtt ctcctctctt gctgaattga
     4081 aacgattcag cttctatgag aaaaaaagcg ccaacctggc ttagggttaa agacaagatc
     4141 gcgctgaaac gtttcaagaa agcataatac ttctgtttca gcacgcaagg aaagccgtaa
     4201 agttatgagc aaaagtttga tgtgctgcac aattttttgt catttttcct aattgctgat
     4261 gggaaaatcc gttatcagca atttcatttc agcagcgtca gcagccaatc agcaacagtg
     4321 aaatggcatt ttgattattc gtggcgcttt tgatttcgct gtctgtttat tttctgacaa
     4381 gcagcgtaaa ctccgcgtct tcctcttcca gtgatcgacc agcatgcata actcccccgc
     4441 agtctccagc gcgaaatcgt ttgacctgac ctcgacggcg tttttaatcg ttgcctttct
     4501 caccggtatt gcgggcgctc tgcaaacccc gacactcagt atttttctta ccgatgaagt
     4561 acatgcccgt ccggcgatgg tgggattctt ctttaccggc agcgctgtca ttgggattct
     4621 ggtaagtcag tttctcgccg ggcgctctga taagcgcggc gatcgcaaat cgctgattgt
     4681 cttttgctgc ctgttaggcg tgctggcctg cacccttttt gcctggaatc gcaactactt
     4741 tgttttgcta ttcgttggcg tctttcttag cagctttggc tcgaccgcta acccgcaaat
     4801 gtttgccctt gcccgtgaac atgccgacaa aaccggacgt gaggcggtga tgttcagctc
     4861 ttttttacgc gctcaggttt cactggcatg ggtcattggc ccaccgctgg cttatgcgtt
     4921 agcgatgggt ttcagcttta cggtaatgta tctgagcgca gcggtagcgt ttattgtttg
     4981 cggcgtgatg gtgtggctgt ttttaccgtc gatgcaaaaa gagcttccgc tggcgaccgg
     5041 cacggttgaa gcgccgcgcc gtaaccgtcg cgatacgcta ctgctgtttg tcatttgtac
     5101 attaatgtgg ggctcgaaca gcctgtacat catcaacatg ccgctattta ttatcaacga
     5161 actgcatctt cccgagaaac tggccggtgt gatgatgggg accgccgccg ggctggaaat
     5221 cccgacgatg ttgattgccg gatatttcgc caaacgtctg ggtaagcgtt tcttaatgcg
     5281 cgttgctgcc gtgggtggcg tctgttttta cgcaggaatg ctgatggcgc attcacctgt
     5341 cattctgttg ggcttgcagc tgctaaatgc tatttttatt ggcattctgg gcggcatcgg
     5401 gatgctctat tttcaggatc tgatgcccgg tcaggcgggt tcagccacca cgctctatac
     5461 caacacttcg cgcgtgggct ggatcatcgc aggatcagtg gcgggcatcg tcgccgagat
     5521 ctggaattat cacgctgtgt tctggtttgc gatggtgatg attatcgcca ctctgttttg
     5581 cttactgcgg attaaagatg tttaaggcgc ggtcagcatt tccagatcga taagccaggt
     5641 catggcctgc tgacgcgagt taccgcacat ttcagcgctg gcttgtaagc ctgcacagac
     5701 tttcggtcgc agtggcgagg tgaaaatttt acagcgttgc tgttcatcaa gctgaatgca
     5761 gggcgtattg gcgggcttgc catctggcat accgggaata gggctggaaa ttgaaggggc
     5821 agtgcaacac gccccgcaac ccggacggca ttccatacgt tgttctctta aggttaacca
     5881 ccggattgat gagcgcgcac agtaccacct tttttgcacc agcaaaagtg cgaataccac
     5941 ttgcccgaaa ggcccgtcgc gagtactttg tcgcgatatt tttgacattt tcgactacag
     6001 gaatttttcg atgccaagag cgaacgaaat taaaaaaggt atggtactga attacaacgg
     6061 caaactgctg ctggttaagg atattgatat tcagtcgccc actgcccgcg gtgccgctac
     6121 gctgtacaaa atgcgttttt ctgatgtccg taccgggctg aaagtagaag agcgtttcaa
     6181 aggtgatgat atcgttgaca ccgtgacgct gacccgccgt tacgttgact tctcctatgt
     6241 cgatggcaac gaatatgtct ttatggataa agaagactat accccgtata ccttcaccaa
     6301 agatcagatt gaagaagagt tgctgtttat gccagagggc ggcatgccgg acatgcaggt
     6361 gctgacctgg gacggtcaac tgctggcgct tgagctgccg cagaccgttg atctggaaat
     6421 cgttgaaacc gcaccaggca tcaaaggggc atctgccagc gcccgtaaca aaccggcgac
     6481 attgagcact ggtctggtga ttcaggtacc agaatactta agcccgggcg aaaaaattcg
     6541 tatccatatc gaagaacgcc gttatatggg gcgtgctgac taacttcagc cgcatgcaga
     6601 aaagggatag ctcaggctgt ccctttttta atttattata ccaatcttct attttgcgct
     6661 tcctgaacac cttacgccct caattttcac tcgttgattg atcgccctca cacttcatcg
     6721 cattaacaat ccagaccaat ttcaattgct gtcatataac tttacactgt cattgttaat
     6781 ttatcgttac taagacgtga ctcctatgaa gacaattgcc tccgttacgc tcccgcatca
     6841 tgtacacgct ccacgctacg atcgccagca gttgcaatca cgtatcgttc attttggctt
     6901 tggcgccttt caccgcgctc atcaggcgtt actgaccgat cgtgtgctga atgcccaggg
     6961 cggcgactgg gggatctgtg aaatcagctt gttcagcggt gatcaactga tgagccagct
     7021 ccgcgcacag aaccatttat ataccgtgct ggagaaaggt gcggacggca atcaggtgat
     7081 aattgtcggt gccgttcacg aatgccttaa tgcaaaactg gattccttag cggcaattat
     7141 tgagaaattt tgcgagccac aggtggcaat tgtttccctg acgattaccg aaaaaggcta
     7201 ttgtattgac ccggccaccg gtgcactcga caccagtaat ccgcggatta ttcacgatct
     7261 acaaacccct gaagaacctc actccgcacc gggtattctc gtcgaagcac tgaaacgccg
     7321 ccgtgagcgc ggccttacac cgtttaccgt gctctcctgc gacaatattc ccgacaatgg
     7381 tcatgtggtg aaaaacgcgg tgctgggaat ggcagaaaaa cgttcgccag aactcgccgg
     7441 gtggataaaa gagcacgtca gttttccggg aaccatggtc gaccgcattg ttccggctgc
     7501 aaccgacgaa tcactggtgg aaatcagcca gcatctgggg gtgaatgatc cctgcgcgat
     7561 tagctgcgaa ccgtttatcc agtgggtggt ggaagataac ttcgtcgctg ggcgtcctgc
     7621 ctaggaagtc gcaggtgtac aaatggtgaa tgatgtcctg ccatgggaag agatgaaact
     7681 gcggatgctt aatggcagcc actcttttct cgcttatctg ggttacctct caggattcgc
     7741 ccatatcagt gattgcatgc aggatcgcgc atttcgccat gccgccagaa cattaatgct
     7801 ggatgagcaa gcgccgacac tgcaaattaa agatgtcgat ttaacacaat atgcggataa
     7861 gttaattgca cgttttgcta atccggcgct gaaacataag acctggcaaa tcgcgatgga
     7921 tggcagccag aaattaccgc aacgcatgct ggcaggtatt cgcatacatc aggggcgcga
     7981 aacggactgg tcgttgctgg cattaggcgt tgcaggctgg atgcgttacg tcagcggcgt
     8041 tgatgatgcc ggaaatgcca ttgatgttcg cgatccgctt agcgataaaa ttcgcgaact
     8101 tgttgcgggc agcagcagtg aacaacgcgt aaccgccctg ctttccctgc gtgaagtttt
     8161 cggtgatgat ctgccagata acccgcattt tgtgcaggcc atcgaacaag cctggcaaca
     8221 aatcgtacaa ttcggcgcac atcaggcgct attaaacacc ctcaaaattt aacgatttct
     8281 gcggttaaag cggatgaagc tcaccttcgt ccgctctccc cttctctttt ctgctttttt
     8341 tagccaggat taacgctcag ttaacttacc agagtattgc ggagccgttg tgaccaggac
     8401 caacctcatc accggttttc tcggcagcgg gaaaaccacg tcgattcttc atctgttagc
     8461 ccataaagat cccaacgaaa aatgggcggt actggttaat gaatttgggg aagtcggaat
     8521 tgatggtgct ttgctcgccg atagcggcgc attgctgaaa gagatccccg gcggctgcat
     8581 gtgctgcgtt aatggtttac ccatgcaggt agggttgaat accttactgc gtcagggaaa
     8641 accagaccgc ttgttgatag agccgaccgg gctgggccat ccgaaacaga tcctcgatct
     8701 gttaaccgca ccagtctatg aaccgtggat agatctgcgc gccaccttgt gcattctcga
     8761 tccgcgcttg ctgctggacg aaaaaagcgc cagcaatgaa aacttccgtg accagctggc
     8821 tgccgcagac atcattgtcg ccaataaatc cgaccgtgcg acgcccgaaa gtgagcaagc
     8881 gctacagcgt tggtggcagc aaaatggtgg cgatcgacaa ttaattcaca gtgagcatgg
     8941 gaaagttgac ggtcatcttc tggatttgcc gcgtcgcaat ttagccgagt tgcccgccag
     9001 cgccgcgcat tctcatcagc atgtcgtgaa aaaagggtta gcagcgttaa gcctgccaga
     9061 gcatcaacgc tggcgtcgca gtctgaacag cgggcaagga tatcaggcct gcggctggat
     9121 attcgacgct gatacggtat tcgacaccat tggcattctg gaatgggcgc gacttgcacc
     9181 ggtggaacgc gtcaaaggcg tgctgcgtat tcccgaaggg ctggtacgaa tcaaccgtca
     9241 gggcgatgac ctgcacattg aaacgcaaaa cgttgcgcca ccggacagcc gtattgagct
     9301 gatttccagc agcgaagctg actggaatgc tttgcagagc gcgctgttga agcttcgttt
     9361 agcgactacc gcgtaaggtt gcctgcgttt ttcagtaaga taattagaga aaatatgatt
     9421 aaaaatttgc cgcaaatagt gttgttgaat attgtcggcc tcgcgctgtt tctttcctgg
     9481 tatattcccg ttaatcatgg attctggttg ccgattgatg cggatatttt ttatttcttt
     9541 aatcagaaac tggtcgaaag taaggccttt ttgtggctgg ttgcattgac caacaatcgc
     9601 gctttcgacg gttgttctct gctggcgatg ggaatgttga tgctgagttt ctggctgaaa
     9661 gaaaacgccc ctggcagacg acgtatcgtg attatgggtc tggtcatgct attaactgca
     9721 gtggtattaa accagctggg tcaggcatta attcctgtaa aacgggccag cccaacattg
     9781 acttttaccg atattaaccg cgtcagcgaa ctgctctctg ttcccacgaa agatgcctca
     9841 cgagatagct ttcccggcga tcacggcatg atgctgctta ttttttcggc attcatgtgg
     9901 cgttatttcg gcaaagttgc aggccttatc gcccttatta tttttgtggt ttttgcattt
     9961 cccagagtaa tgattggcgc acactggttt actgacatca ttgtcggttc gatgaccgtg
    10021 atattgatcg gtttgccctg ggtgttgctg acgccattaa gtgatcgatt aatcaccttt
    10081 tttgacaaat cactaccggg aaaaaacaaa catttccaaa acaaataact cacagtaatt
    10141 aacatcatca ggcttatttt tatagtgagg ataatcctga tgatgcgcac cgtgctttca
    10201 tccatcgcgc gcaaaaatca ttctctaagt aaatgaatgg attgcatgcg tttcactcaa
    10261 tcgtacttta attgaccaat cccgcttatt aactttctgt atcacttttt cttataaaaa
    10321 atcatgtaaa accgctcgcc aagaccgcac caatcgggta atctcgaact cgttttgcct
    10381 cggcggtaga ttatcctcac agcatataat tttgtgcgtt agtccacaga tttggcctta
    10441 aggaattgtt tcaacatgcc caggtaatta gtctcgtgtc gcttggcatt tttttataac
    10501 gatatttgtc gttaaggact tcaagggaaa acaaacaaca tggtcaaatc tcaaccgatt
    10561 ttgagatata tcttgcgcgg gatccccgcg attgcagtag cggttctgct ttctgcatgt
    10621 agtgcaaata acaccgcaaa gaatatgcat cctgagacac gtgcagtggg tagtgaaaca
    10681 tcatcactgc aagcttctca ggatgaattt gaaaacctgg ttcgtaatgt cgacgtaaaa
    10741 tcgcgaatta tggatcagta tgctgactgg aaaggcgtac gttatcgtct gggcggcagc
    10801 actaaaaaag gtatcgattg ttctggtttt gtacaacgta cattccgtga gcaatttggc
    10861 ttagaacttc cgcgttcgac ttacgaacag caggaaatgg gtaaatctgt ttcccgcagt
    10921 aatttgcgta cgggtgattt agttctgttc cgtgccggtt caacgggacg ccatgtcggt
    10981 atttatatcg gcaacaatca gtttgtccat gcttccacca gcagtggtgt tattatttcc
    11041 agcatgaatg aaccgtactg gaagaagcgt tacaacgaag cacgccgggt tctcagccgc
    11101 agctaataaa ccgtttggat gcaatccctt ggctatcctg acgagttaac tgaaagcact
    11161 gcttaggcag tgcttttttg ttttcattca tcagagaaaa tgatgtttcc gcgtcttgat
    11221 ccaggctata gtccggtcat tgttatcttt taaatgttgt cgtaatttca ggaaattaac
    11281 ggaatcatgt tcatacgcgc tcccaattct ggacgtaagc tcctgcttac ctgcattgtt
    11341 gcaggcgtga tgattgcgat actggtgagt tgccttcaat ttttagtggc ctggcataag
    11401 cacgaagtca aatacgacac actgattatc gacgtacaaa agtatctcga tacctatttt
    11461 gccgacctga aatccactac tgaccggctt cagccgctga ccttagatac ctgccagcag
    11521 gctaaccccg aactgaccgc tcgcgcggcg tttagcatga atgtccgtac gtttgtgctg
    11581 gtgaaagata aaaaaacatt ctgttcatct gcgactggtg agatggacat tccactcaat
    11641 gaattgattc cggcgctcga cattaataaa aacgtcgata tggcgatctt acccggcacg
    11701 ccgatggtgc cgaacaaacc cgcaatcgtc atctggtatc gcaacccttt gctgaaaaat
    11761 agcggtgtgt ttgccgctct gaatctcaac ctgacgcctt ctctctttta tagttcacgg
    11821 caggaagatt acgatggcgt cgccctcatt attggcaata ctgcgctatc taccttttct
    11881 tcacgtttga tgaacgttaa tgaattaacc gacatgccgg tccgtgaaac taaaattgcg
    11941 ggcattcctc tgaccgttcg gctttatgcg gatgactgga catggaacga tgtgtggtac
    12001 gcatttttac tgggcggcat gagtggaact gtcgttggcc tgctctgcta ttacctgatg
    12061 agcgtacgta tgcgccccgg cagagaaatc atgaccgcca tcaagcgcga acaattttac
    12121 gtggcgtatc aaccggtggt ggatacacaa gctttgcgag taacgggcct ggaagtactg
    12181 ctacgctggc ggcatcctgt cgcgggagaa attcccccgg atgccttcat taactttgcc
    12241 gaatcgcaaa agatgattgt gccgctgact cagcacctgt ttgagttaat tgcccgcgat
    12301 gccgcagaat tagaaaaagt gctgccagta ggcgtcaaat ttggtattaa cattgcgccg
    12361 gaccatctgc acagcgaaag ctttaaagca gatatccaga aactgctcac ttccctaccc
    12421 gcacaccatt tccagattgt gctggaaatt accgagcgcg atatgctgaa agagcaagaa
    12481 gccacacaac tcttcgcctg gctgcactcg gtcggcgtag aaattgctat tgatgacttc
    12541 ggcaccgggc acagcgcgct tatctatctt gagcgtttta cgctcgatta tctgaaaatt
    12601 gaccgtggat ttatcaacgc catcggtacg gaaacgatca cttcacccgt acttgacgcg
    12661 gtgctgacgc tggcgaaacg tctcaatatg ctgacagttg ctgaaggggt cgaaacgccg
    12721 gaacaagcgc gatggctaag cgaacgcggc gttaatttca tgcaaggcta ctggattagt
    12781 cgcccgttac cgctggacga ttttgtccgc tggctgaaga aaccgtatac gccgcagtgg
    12841 taaggtgtgc ttacgtccct tattattcat agtgaaagca tgccggattg cggctaatga
    12901 tgagtaaaag gaaatccgtt gcagatgatt gtgcgcatac tgctgctgtt tatcgctctg
    12961 ttcacctttg gtgtgcaggc gcaggctatc aaggaaagct atgcctttgc cgtgctgggc
    13021 gaaccccggt acgcgtttaa tttcaaccat tttgattatg tgaaccccgc cgcgccaaaa
    13081 ggtgggcaga taacgttgtc agccctcggc accttcgata ctttcaaccg ctatgcactg
    13141 cgcggcaacc cgggcgcacg caccgagcag ctgtacgaca cgctatttac gacttccgat
    13201 gacgaaccag gcagttatta cccgctgatt gctgaaagcg cacgctatgc tgacgattat
    13261 tcctgggtgg aggtcgctat taatccgcgc gcccgttttc atgatggttc gcccattact
    13321 gcccgcgatg tagagtttac ttttcaaaaa tttatgaccg aaggcgtgcc gcaatttcgt
    13381 ctggtctaca aaggcaccac cgtcaaagcc attgcgccgt taaccgtgcg cattgagtta
    13441 gctaaacccg gcaaagaaga tatgttgagt ctgttttcgc tgccggtatt tccagaaaag
    13501 tactggaagg atcacaaact tagcgacccg ctcgccacgc ctccgcttgc cagtggtccg
    13561 taccgcatta cgtcctggaa aatggggcaa aatattgtct attcccgcgt gaaagattac
    13621 tgggcagcaa acttaccggt aaaccgcgga cgctggaatt tcgacaccat tcgctacgat
    13681 tattacctcg atgataatgt cgcctttgaa gcgtttaaag caggtgcctt tgatttgcgt
    13741 atggaaaacg acgccaaaaa ctgggccacg cgttataccg gtaaaaattt cgataaaaaa
    13801 tacatcatca aagatgagca aaagaacgaa tcagcccagg atacgcgctg gctggcgttt
    13861 aatatccaac gtccagtatt cagcgatcgc cgggtgcggg aagcaatcac tctcgccttt
    13921 gactttgaat ggatgaacaa ggcgttgttt tacaatgcct ggagtcgcac gaacagttat
    13981 tttcagaata ccgaatacgc ggccagaaat taccccgacg ccgcggagct ggtgcttctg
    14041 gcaccaatga aaaaagatct accgccagaa gtcttcacac aaatctacca gccgccggta
    14101 tccaaaggcg atggctacga tcgtgacaac ctgttaaaag ccgacaaact tctcaacgaa
    14161 gcgggctggg tgctgaaggg tcagcaacgc gttaatgcca caacgggtca gccactcagc
    14221 tttgaattat tgcttcccgc aagcagcaat agtcagtggg tattgccgtt ccagcacagc
    14281 ctgcaacggc tgggtatcaa catggacatt cgcaaggtgg ataactcgca aatcactaac
    14341 cgcatgcgca gtcgcgacta tgacatgatg ccgcgcgtat ggcgggcgat gccgtggccc
    14401 agttccgatt tacagatttc ctggtcatcg gaatatatca attccactta taatgccccc
    14461 ggcgtgcaaa gcccggttat cgactcgctg atcaaccaaa ttattgccgc gcagggaaat
    14521 aaagaaaaat tactgccgtt ggggcgagca ctggatcgcg tattaacgtg gaattattac
    14581 atgctgccaa tgtggtacat ggcggaagac cgtctcgcct ggtgggataa attctcccag
    14641 ccggccgtgc gccccatcta tagcctcggt atcgatacct ggtggtatga cgtcaataaa
    14701 gcggccaaac tgccgtccgc cagcaaacag ggagagtaga tgggcgctta cctgattcgc
    14761 cgtctgttgc tggtgatccc aacattatgg gcgattatca ccatcaactt tttcatcgtg
    14821 caaattgcgc ctggcggtcc ggtcgaccag gccatcgccg ccattgagtt tggtaatgcc
    14881 ggagtattac ccggcgcagg cggtgaaggt gttcgtgcca gccatgcgca aacgggtgtc
    14941 ggcaatatca gcgacagtaa gtaccgtggc ggacgcggat tagatccaga agtgatcgct
    15001 gagatcactc atcgctacgg ttttgataag ccgatccacg aacgttactt caaaatgctc
    15061 tgggactaca tccgctttga ttttggcgat agcctgtttc gcagcgcctc ggtgctgacg
    15121 ctgattaaag acagtctgcc ggtttccatc accctcggat tgtggagcac gctgattatc
    15181 tatctggtgt cgattccgtt aggcattcgc aaagctgttt ataatgggag ccgctttgac
    15241 gtctggagta gcgcatttat catcatcggc tacgccattc cggccttttt gtttgccatc
    15301 ctgctgattg tcttcttcgc gggcggcagc tatttcgacc tgttccctct acgcggcctg
    15361 gtttccgcta actttgattc gctgccgtgg tatcagaaaa tcaccgatta tctgtggcat
    15421 atcacgctgc cggtgctggc gacagtgatt ggtggctttg cggcgctgac catgctgaca
    15481 aaaaactcat tccttgatga agtgcgcaag caatacgtgg tgaccgcgcg tgcgaaaggg
    15541 gtaagtgaaa aaaatattct ctggaaacat gtgttccgca acgccatgct gctggtgatt
    15601 gccggttttc cggcgacgtt tatcagcatg ttttttaccg gctcgctgct gattgaggtg
    15661 atgttttcac tcaatggtct gggcttactg ggctacgaag cgaccgtctc gcgcgattat
    15721 cctgtaatgt ttggtacctt gtatattttc accctgattg gcctgctgct gaatattgtc
    15781 agtgatatca gctatacgct ggtcgatccg cgtatagatt ttgagggacg ttaatgtcgc
    15841 gactcagccc cgtcaatcag gcccgttggg cgcgttttcg ccataaccgt cgcggctact
    15901 ggtcgttatg gattttcctc gttttgtttg gtttgagttt gtgttctgaa cttatcgcca
    15961 acgataaacc gttgctggta cgttatgacg gcagttggta tttcccgttg ttgaaaaact
    16021 acagcgaaag cgattttggc ggcccgctgg caagtcaggc tgattatcag gacccgtggc
    16081 tgaaacaacg gctggaaaat aacggctggg tactgtgggc accgattcgc tttggtgcta
    16141 ccagtatcaa ctttgctacc gataagccct tcccttctcc accctcccgg caaaactggc
    16201 tgggaacgga tgccaacggc ggcgatgtgc tggcacgtat tctctatggc acgcggatct
    16261 cggttctgtt tggcctgatg ctgactctct gttccagcgt gatgggcgtg ctggcggggg
    16321 cgctacaagg ctattacggc ggtaaagtcg atctctgggg gcaacgcttt attgaagtat
    16381 ggtcggggat gccgacgctg tttttgatta ttttactttc cagcgtcgta cagcctaact
    16441 tctggtggct gctggcaatt actgtcttgt ttggctggat gagtctggtc ggcgtggtgc
    16501 gggcggagtt tttacgcacc cgtaatttcg actacattcg tgcggcgcag gcgcttggcg
    16561 tcagcgatcg cagtatcatc ctgcgtcata tgttgcctaa tgccatggtc gcgaccctca
    16621 cctttttacc gtttatttta tgtagttcga taaccaccct gacctcactc gatttcctcg
    16681 gcttcggtct gccgctcggt tcaccgtcac tcggtgaact gctgttacaa gggaaaaata
    16741 accttcaggc tccgtggctt gggatcaccg ccttcttgtc ggtggcgata ttattgtctt
    16801 tgctgatctt tattggtgaa gccgtccgcg acgcatttga tcctaataag gcggtgtagc
    16861 atgacgcaaa ctctgttagc gattgaagat ttgtcggtgg gttttcgcca tcagcaaacc
    16921 gtacgtacag tagtcaatga tgtttcacta cagattgagg ctggcgaaac gctggcgctg
    16981 gtgggtgagt caggttcagg caaaagcgtt accgcgctgt caattttacg cctgctccct
    17041 tccccgccgg ttgaatatct ctccggcgat attcgttttc gtggcgaatc gctgcttcat
    17101 gccagcgatc aaacgttgcg cggtgtacgc ggtaataaga tcgccatgat ttttcaagag
    17161 ccgatggtgt cgttaaatcc attgcatacc ctggaaaaac agctttatga agtgctttca
    17221 ctccaccgcg ggatgcgtcg ggaagcggct cgaggcgaaa ttcttaactg ccttgatcgc
    17281 gttggtatcc gccaggcggc aaaacggctg acagattatc cgcatcagct ctccggcggc
    17341 gaacgccagc gggtgatgat tgcgatggcg ctgttaacgc gaccggaatt attaattgcc
    17401 gatgaaccga ccaccgcact ggacgtctct gtccaggcgc agattttaca gctgttgcgc
    17461 gaactgcaag gcgagctgaa tatgggcatg ctgtttatta ctcataacct cagcattgtc
    17521 agaaaactgg cccaccgcgt ggcggtaatg caaaacggtc gctgtgtcga gcaaaataac
    17581 gccgctacgc tatttgcctc acccactcat ccttacacac aaaagctact caacagtgaa
    17641 ccgtcaggcg acccggtgcc gttgccagaa cctgcctcaa cgttgctgga tgttgaacag
    17701 cttcaggttg ccttccccat tcgcaaaggg attttgaagc gcattgtgga tcataatgtg
    17761 gtggtgaaaa acatcagttt tacgctacga gcgggtgaaa cactgggttt agtgggcgag
    17821 tccggttccg ggaaaagtac gacgggactg gcgctgctgc gactgattaa ttctcagggc
    17881 agcatcatct ttgacggtca gccactgcaa aatttaaatc gccgccagct gttacctatt
    17941 cgtcatcgca ttcaggtggt atttcaggat ccaaactcct cgctcaaccc acgactcaac
    18001 gttttgcaga ttattgagga aggcttacgg gttcaccagc cgacgctttc tgccgcacaa
    18061 cgcgaacaac aagtgatagc cgtgatgcat gaagtgggat tagatcctga aacacgccac
    18121 cgttatccgg cggagttctc tggtggtcag cgacaacgta ttgcgattgc cagggcatta
    18181 attcttaagc cctcgctgat catacttgat gaaccgacat catcactcga caaaacggta
    18241 caggcgcaaa tattgacgct attgaaatca ttgcaacaaa agcatcaact ggcctatttg
    18301 tttatcagcc acgatttgca cgttgtccgc gcgttatgtc atcaggttat cgtactgcga
    18361 caaggggaag tagtggaaca aggaccgtgc gcgcgcgtgt ttgccacacc gcagcaggag
    18421 tatacgcgtc agctactggc gttgagctga cgcttaaaaa ggattgtagt ttgaaaaggg
    18481 ttcggcaatt gccacaccaa aatttttcaa tcggcatgtt gcagcaaact cgtcctggcg
    18541 atttacaaac aggcacggct caccttcaca ctccagcacc gaacatggaa cttcgatgtc
    18601 gcttaacgcc tggcttagtt tatacatcac cgaccatgca ttatcaccat ccgggctaag
    18661 caatttaaga gccaccaggc tgttgtcacc gcacggtcca ttttgcggaa tcggttcaac
    18721 cttcgaacct gcgaaaccag cagaccagcg atagttctgc ggcagtcgat ggacgattga
    18781 gagttgtaat gaagtcactt attttccccg gaagcacatt cacttcacaa tttgtttaca
    18841 tcaattttaa cacatcatca acaatccgtc tttatacaga tcgtaaagat tgatgcctcg
    18901 cctgcgtggc ctctatggct ctgatttaag taatatctgc gggcgtggtc acgtttgcgg
    18961 ggctatatgt acccgtttct gcgatctaac tcaacctttt taactacaat gatgtgactt
    19021 tttacataaa ttgattttac ataaaataaa catatatcgg gggaatgata gatttgtggt
    19081 tgacatgcat caacaaagga agccttttag cttcctcgtt gtgcaataga tcaccgtttt
    19141 ttcggtcgac tggcgtacag acagaagaga atggagctgc ttgcgcagaa tgcaattgac
    19201 caaatcatcg gccatgcaga gttaaaggtc gcaagagaaa gcaatgcgcc aacaattgcc
    19261 cctatgccaa aacggaaggt tcctgccagc gaagatgccg ttcccgccat atggggaaac
    19321 tcatcaagaa tgaccgccat cgcattggat gacaccatcg acacgcagcc cacaaacgcc
    19381 gcaacgccaa ccaccagcga ccagaatccc agccccagca gcgcactgat gaccatccac
    19441 gctgccataa taaattgtat ccacaacccc gagcggaaca tatttaacgc gccaatgcgg
    19501 cgaacgaagc ggctgttaaa aatggtcatc acgaacagga aaacaatgtt tagtgcaaag
    19561 taataaccaa agttttccgg cgcgacgtgg ttaatttcaa tgtagacaaa cggtccggcg
    19621 cttaagaacg agaacatccc ggcaaagctg aaaccactgg caagcatata actcaggaca
    19681 cgcttatgac ggaacagcgc cgcaaagtta ccaatagtgg tacgaatgtg aaaaggctgg
    19741 cgacgctccg gtggtaaggt ttctttaatc aggaagaaaa tcattgccga agccagaatc
    19801 gccgctaatg ccaggatcca gaagatgtaa tgccagctca gccacaccag cacccaaccg
    19861 ccgacaatcg gtgccatcag cggtgcaata gtggtcacca gcatgacaaa cgacatcatc
    19921 cgcgagaact cttctttcgg gtaaatatcg cgcatcaggg cgttaatgac cacgctggcc
    19981 gcagccgccg ccagcccgtg gaagaaacgc atcacaatca gctgatcgat ggtgtttgcc
    20041 aacgcacacg ccaccgcggc ggcggcaaac accagcgtac cgccgagcac caccggctta
    20101 cgcccgaagc tgtctgccat cggcccgtag attaactgcc ccaacgcaaa gcccagaata
    20161 taagtactga gggtcatctg cgtactgccc gcaggtacgc caaactgcgc tgaaattacc
    20221 ggtagcgcgg gcagatacat atcaatcgac agcggcatca acatggccag caggccaagg
    20281 ataaaaacaa tagcaaacga cgaatgctgt cgggtggtca caacgggctc ctgaaagtca
    20341 tggaaaagtt agacgacgct ggcaatttct tcttcagtta acggacgata ttcaccgggg
    20401 gctaaatcag catccagcgt aataccgcca atacgttcac gatgcagctc aaccacgtgg
    20461 ttacccacgg cggcgaacat gcgtttcacc tgatgataac gcccttcgct gatggtcaga
    20521 cgaacctgcg ttggggtaat cacttccagc accgcaggct tagtgagatc tttttcgtta
    20581 tgcagctgca cgcctttagc aaattgctct gccgtatcgt cagctacagg tgattccagt
    20641 gtcaccagat aggtcttctc gcaatgatgg cgcggagaag taatgcggtg cgaccactga
    20701 ccatcatcag tcatcagcac cagaccggtg gtatcgatat ccagacgtcc tgcggcgtgc
    20761 agtttccagg ctaccggttc atccagaaaa tagagcaccg ttgggtgatc agggtcgtcc
    20821 gtggagcaaa catagccctg aggcttattg agcatgaagt aacgtggacc atgttgctgc
    20881 gccagcgggt tgccatcgta agcgacatca tgttcaggaa gcagtttgaa cgctgcatta
    20941 cgaacaattt cgccatcgac ggtgacacga ttgccgcgga tttcacgccc ggcaatagca
    21001 cggctaacgc cgagttgctg tgcgataaat ttatcaagtc gcatgtgtgt gattttgcct
    21061 gtaaaaacgg aggtcgggca ttgacccgaa aatctgaact gttgtctgcc cagtatagcg
    21121 gtctaataac atccctcaag ggaaaaagat ccatggcata ctattagcag aataatctac
    21181 ctacgcgaga ccatgatttt tacactccgc ccatatcagc aagaagccgt ggatgccacg
    21241 ctcaaccatt ttcgtcgtca taaaacccct gccgttatcg tgctgcccac cggcgcaggt
    21301 aaaagcctgg tgattgccga actggcacgg ctggcgcgtg gtcgtgttct ggtgctggca
    21361 cacgtcaaag aactggtggc gcaaaaccat gcaaagtatc aggcgctggg gctggaagcc
    21421 gatatttttg ccgctgggtt aaagcgcaaa gagagccacg gtaaagtggt atttggcagc
    21481 gtgcagtcgg tcgctcgtaa tcttgatgcc tttcagggtg aattttcgct gttgattgtc
    21541 gatgaatgcc accgtattgg tgacgatgaa gagagccagt atcagcaaat cctcactcac
    21601 ctgactaaag tgaatcccca cttacgcctg ctggggctga ctgccacgcc ttttcgattg
    21661 ggcaaaggct ggatctacca gttccattat cacggcatgg tacgcggcga tgagaaagcc
    21721 cttttccgtg actgcattta tgagctgccg ctgcgttata tgattaaaca cggctatctg
    21781 acgccgccag aacgactgga tatgccagta gtgcaatacg atttcagccg cttgcaggca
    21841 cagagtaacg ggctgttcag cgaagctgat ctcaaccgtg agctgaaaaa acagcaacgc
    21901 attacgccac atatcatcag ccagatcatg gagttcgccg aaaagcgcaa aggggtaatg
    21961 attttcgccg ccaccgttga acacgcaaaa gagattgtgg gattactgcc tgccgaagat
    22021 gctgcactga ttactggcga cacccccggc gctgagcgcg atgtgttaat tgaagatttt
    22081 aaagcccagc gttttcgcta tctggtcaac gtcgcagtac tgaccaccgg atttgacgcc
    22141 ccgcacgtcg atcttatcgc cattctgcgc cctaccgaat cagtgagtct ttaccaacaa
    22201 attgtcgggc gcggtctgcg tctcgctccg ggcaagactg attgtttaat tcttgattat
    22261 gcgggtaatc ctcacgatct ctacgcgccg gaagttggta caccgaaagg caaaagtgac
    22321 aacgttccgg tacaggtttt ctgccctgcc tgcggttttg ccaacacctt ttgggggaaa
    22381 acgaccgccg acgggacatt gattgaacac tttggtcgcc gctgtcaggg atggtttgaa
    22441 gatgacgacg gtcatcgcga acagtgtgac ttccgtttcc gttttaaaaa ttgcccgcaa
    22501 tgtaacgcag aaaatgatat tgccgctcgc cgctgccgcg aatgtgacac ggtactggtt
    22561 gatccggatg atatgttaaa agcggcgcta cgactgaaag atgcgctggt attacgctgt
    22621 agcggcatgt ctttgcagca tgggcacgac gagaaaggcg aatggttgaa aatcacctat
    22681 tacgatgaag acggcgcgga tgtgagtgag cgtttccgtc tgcaaacgcc cgcccagcga
    22741 actgccttcg agcagctttt tatccgcccg catacgcgca caccgggcat ccctctgcgc
    22801 tggatcaccg ccgccgatat cctcgcccag caagccttac tgcgacatcc ggattttgtc
    22861 gtcgcccgca tgaaaggtca gtactggcag gtgcgtgaaa aagtgttcga ttacgaaggt
    22921 cgttttcgtc gggcgcacga attacgcggt taatggtagt tttgattgat gtataaggcg
    22981 attgagtata gaatctcgcc cgcttttgca tacgcaaagc agatcactta cctgttgctg
    23041 ggtcgcctgt agcaggatta atttaagaga gaaagaaatg tttactatca acgcagaagt
    23101 acgtaaagag cagggtaagg gtgcgagccg ccgcctgcgt gccgctaaca agttcccggc
    23161 aatcatctac ggtggcaaag aagcaccgct ggctatcgag ctggatcacg acaaagtcat
    23221 gaacatgcaa gctaaagctg aattctacag cgaagttctg accatcgttg ttgacggtaa
    23281 agaaatcaaa gttaaagctc aggacgtaca gcgtcacccg tacaaaccga agctgcagca
    23341 catcgacttc gttcgcgctt aattgctgaa taagttgtaa aaaaccccgc tccggcgggg
    23401 ttttttgtat ctgcagatta tgcctgatta cggtattgct attttttgca ggccagataa
    23461 ggcgttcacg ccgcatctgg cattcagaac acaaaaccga ttaattgccg ccagacgtcc
    23521 ggcgctgcaa ttggtcgcgc aaattcggcg gcgtgccttt aatggtcaag gtatcggttg
    23581 ctggatccca gaagatgcgt tcacctaaca gcatcgcatc aaagttaata gtcaagccac
    23641 cgccgctacc ggcaaacttc gtcaactgac gcagtgtgct acggtctgcc gggaagctct
    23701 cttccagttc gtagcctttt tcagccgcaa actccgtgaa gctcacttcg cttacgcctg
    23761 ccagctcttt cgacagcgat tccagttcga tctcttcgcc tgcctgcagc tgctcattac
    23821 agtagctata aacctgctgg cgcacgttct gtcgctctgc tttatccagt tgtgcttcgg
    23881 cagtgaaatc atcaaccgct tgcaacagcc cacggttttg cgctttcgcg ttcaatcctt
    23941 cgctggcccc gaggaaatcc ataaagaaat ccgccacttt gcgccctact cgccctttca
    24001 ggaaagtgag atagcgggtg gactctggat tggtttccca ttcggttaaa tcgatacgcg
    24061 caacaatatc cgcatgattg atatcaagat aatgggttgg gttgatgtcg agattttcgt
    24121 taacgcgcat actgctcagg ttgctcagaa ctgccaccag cagatactcc accgccagat
    24181 aacggtagtg gcaaaacagc acgaatccac cgtcagcaaa aggatatttc gctaattcgt
    24241 cacgcaggcg cccggttgcc gcgcggctaa atgccagaaa gtcctcttcg ccctgacgct
    24301 gtaagcgcag cgtctgcgcc agttcactct cttcgctaaa caagccgtaa gctttatttt
    24361 tggcgctata gacccgatgc agttctgcca ccatctcaac gacggtttct gtcggttcca
    24421 gcaatgaatc gcgcaacacc agctcaaggt tctgctcatc acgcttgata agctggtgca
    24481 gggcaatctg gttgatatcc agactcatga taaactctcc tttaagaccg ggcggtattc
    24541 aaccaccgcc ggttaagcga cgcaataaaa gatggcggtc attttacaga aggcagcgta
    24601 acgagaatga tttaacgcat aaaaaagcag aaaaaaaacc gttgctacgg taatatgttg
    24661 ccctttcatc aactaactga tttcgattta tgccacaaat ttcccgctac agtgatgaac
    24721 aggttgaaca attgctcgca gagctgctca acgtactgga aaaacataag gctccgaccg
    24781 acctttccct gatggtgtta ggcaacatgg tcactaacct tatcaacacc agcattgccc
    24841 cggcccaacg ccaggcaatt gccaactctt ttgcccgcgc cttacagtcc tctatcaacg
    24901 aagacaaagc gcactaaggg aaacagataa ctggttatgg taactcatcg tcagcgctac
    24961 cgtgaaaaag tctcccagat ggtcagttgg gggcactggt ttgcactgtt caatattctg
    25021 ctttcgctcg tcattggcag ccgttacctg tttatcgccg actggccgac aacgcttgct
    25081 ggtcgcattt attcctacgt aagcattatc ggccatttca gcttcctggt gttcgccacc
    25141 tacttgctga tcctcttccc gctgaccttt atcgtcggct cccagaggct gatgaggttt
    25201 ttgtccgtca ttctggcaac ggcgggaatg acgctattac tgatcgatag cgaagtcttt
    25261 actcgtttcc atctccatct taatcccatc gtctggcaac tggttatcaa cccagacgaa
    25321 aatgagatgg cgcgcgactg gcagctgatg ttcatcagcg tgccggttat tttattgctt
    25381 gaactggtgt ttgcgacgtg gagctggcaa aagctgcgca gcctgacgcg tcgtcgacgc
    25441 ttcgcgcgcc cgctggccgc attcttattt atcgccttta tcgcctcgca tgtggtgtat
    25501 atctgggccg atgccaactt ctatcgcccg atcaccatgc agcgcgctaa cctgccgctt
    25561 tcgtacccga tgacggcgcg acgttttctt gagaagcatg gtctgcttga tgcgcaggag
    25621 tatcaacgcc gtcttattga gcaaggtaat ccagacgccg tttccgtaca gtatccgtta
    25681 agcgaactgc gctatcgcga tatgggcacc gggcagaatg tgctgttgat tactgtcgat
    25741 ggcctgaact actcacgctt cgagaagcag atgcctgcgc tggcaggttt tgctgagcaa
    25801 aatatttcgt tcacgcgcca tatgagctcc ggcaacacta cagacaacgg catctttggc
    25861 ctgttctatg gcatctcgcc gagctatatg gacggcattc tgtcgacccg tacgcctgcg
    25921 gcattaatta ctgcgcttaa tcagcaaggc tatcagctgg ggttattctc gtcagatggc
    25981 tttaccagcc cgctgtatcg ccaggcattg ttgtcagatt tctcgatgcc gagcgtacgc
    26041 acccaatccg acgagcagac cgccacgcag tggatcaact ggctgggccg ctacgcacaa
    26101 gaagataacc gctggttctc gtgggtctct ttcaatggca ctaacattga cgacagcaat
    26161 cagcaggcat ttgcacggaa atatagccgg gcggcaggca atgtcgatga ccagatcaac
    26221 cgcgtgctca atgcactgcg tgattctggc aaacaggaca atacggttgt gattatcact
    26281 gccggtcggg gtattccgct gagcgaagac gaagaaacct ttgactggtc ccacggtcat
    26341 ctgcaggtgc cattagtgat tcactggcca ggcacgccgg cgcagcgtat taatgcgctg
    26401 actgatcata ccgatctgat gacgacgctg atgcaacgcc tgctacatgt cagcacacct
    26461 gccagcgaat attcgcaagg tcaggatttg ttcaaccctc aacgccgtca ttactgggtt
    26521 actgcagcgg ataacgatac gctggcaatt accaccccga aaaagacgct ggtgctgaac
    26581 aataacggta aataccgcac ttacaactta cgtggtgaaa gagtgaaaga tgaaaaacca
    26641 cagttaagtt tgttattgca agtgctgaca gacgagaagc gttttatcgc taactgatta
    26701 attataaatc agttagcgaa atatcttact tgcaatcggt gtggaaaacg gtagtattag
    26761 cagccacgag tcggcacgta gcgcagcctg gtagcgcacc gtcatggggt gtcgggggtc
    26821 ggaggttcaa atcctctcgt gccgaccaaa tcgcccaccg gatatcaccg gaaagcaccg
    26881 gataagacag ggataacccg ctaatcactt gattggcggg tttttttatg ttttttagtc
    26941 accgaacatc accggattta taagctatca gccggatttt ttagttatac gtttagttat
    27001 acgggctggc gtataactaa agccgtataa ctaaaaccct gttttttcgt gtggaatcat
    27061 agacatatgg cgagaaaaac acacccatta accacagtgc agatcaaagc agccagacca
    27121 gcgcaaaagg agtacaccct gcaggacggc ggagggcttt ttctcctggt caaaccgtct
    27181 ggatcaaaac tctggagatt ttcctactac cgaccatcgg acaaaaaaag aatattgctg
    27241 agttttggat cgcttgaaga tgtttccctg gctgatgcca gaaaacgccg tagcgagtac
    27301 aggacgttaa tcagtgccgg aactgacccg caggaccacg agaggcaaaa aagagagaca
    27361 gaggcccgaa gacaagggaa cacgttcgaa aatgtggcgg cggcatggta ccaggtgaaa
    27421 atcagccaga atctggcccc caacacgatt aaagacatct ggcgttcgct ggataaatat
    27481 gtattcccgt tcatcggcaa cacgccaata gataccctca ccgcccgaag gttcgttgaa
    27541 gtgcttaccc ccatcaagga gcgcggcaac ctggaaacac tcaagcgggt tttacagcgc
    27601 gttaatgagg taatggatta cgccgccaac agtgggctga ttgatgccaa tccggctatg
    27661 aatgtgcgta aggcgtttcc ctcacctgta aaaaaacata tgccaacaat ccgccccgaa
    27721 cagctgcccg agcttatgca ggctttatca gtatcggcaa cagaacggca gaccagatta
    27781 ctgattgaat ggcagttact gaccgtaacc cgtcccgccg aagcgtcatc aacgcggtgg
    27841 gatgaaatca acctggacgc gaagcaatgg acgatacctg ccggacgcat gaagatgcgc
    27901 agggatcacg ttatcccgct ttccggtcag gctatggcgg tgctggaggc catgaaacca
    27961 atcagccacc accgcaatta cgttttccca agtctgaaag acccacagca gccgatgaac
    28021 agccagacag ctaacgcagc attgcggcgt atgggattcg ctggcgtgct ggtgtctcat
    28081 ggattacgcg ccatattcag cacagcagcg aacgaggaag gattcgagcc ggacgtaata
    28141 gaggcggcac ttgcccacgt cgacaccaac gaagttagac gggcatacaa ccggagcaac
    28201 tacatagaaa aacgcatcgt gctgatgcgc tggtggggcg aatttgtcga ggctgcggcg
    28261 acgggcgtaa ccctcgccag tggtaaaagg ggtatccgag ccgtgtagct gtacagaaaa
    28321 ccagtaaaaa ctacgaaaac catgtaaaac cgtcgtataa ttgcatcaaa tttaacgaca
    28381 aggccgtgaa acatgaaacc gttaagatgc aaaaaaatat cagatgcaat tgcgacgggc
    28441 tgcaactggc cctgatggtt cagcatgaat tttggtcaac ctacgatccg gaggacagaa
    28501 cgacggcccc atcaaaaaaa caggtagtag attttctggt atcccgtggc gcttccagaa
    28561 atctggcggt aagtattgat aaggtcgtac gtccggcatc tatgaagatc ggaggcaggc
    28621 ccaaaaaatg gcggtaacaa tcctggaagc ggcagaaatg ccgctttttt tataattcca
    28681 tttcaaatca tcaatataaa aaacggtgta taccgtttaa aaacggtggg aactgttttt
    28741 acccatatcc gatgatttac cgtatttgtc accggaatac accggattca caaggtaaat
    28801 cacgatggaa gcaatcagaa aaattatctt tcgccaggaa gtaaaaaaaa ttatccacat
    28861 caaggcagac agcacgctgc aaagcatgat caacgccgga gaatttccgc agggttttcg
    28921 cgttggttta cgccgtcgcg gatggtatga ggatgatgtg ttgtcctggc agaaagaacg
    28981 cgaagaggaa gcacgcggaa cggctgctta acgggtatca cagatatgac aaacacgaaa
    29041 aaagcggccc cgatatggag ccgcctttct gaacaattaa cccgctgcgc cttatgtgta
    29101 tgtgatccca aacataagca cggggatgat agccgctatc aggctggtgg gcaatgcaat
    29161 cagtctggtt cagttcgttg ccatacctgc aatgagcgct tttccctgta ctctttaagg
    29221 aattgctcaa gggcaaaagc acatggcgcg aatctttctg attcatgctc tatctttctg
    29281 cgccgtcttt tccgtgccgg tgataatgtt ttggtcaatt ctttatcggt cattgtgttg
    29341 tcctgcatag caatgcgccg taatacctca caccacggcg ctgatagttt ttatcctttg
    29401 ggttctatgc cgcgttgctg taattctttg cggagaacgc gtttaatcca agtggctaaa
    29461 gatgtgtcgc catcggcctt agctgcttct tctagctgcg ctctgaagtc ttccggtaat
    29521 cgcatttggt atggaggcga acgtttttct aatggtgttg acatggtaat tacccttcat
    29581 tactatacct acatggtaat taccattgta cttgccatta cacaaaaatc aacgccccgc
    29641 agtgctggga acacatacgg ggcgtctaac caacaacgta aactaggagc cgttatggtt
    29701 gccgtaaatc atataccaca ccttgtacac acacaaacgg cctttgtgtg gcgttttctg
    29761 gccctgaata tcggagaaaa aaaccagttg atcgccagtg tcgaaaatag ctatgattcc
    29821 cgcgcacctc ataaaacggg tgccgggatt ggcgtcctgg gcaaaactga agcgcataac
    29881 acgcgccccg cgtgtttttt tgtgccgcat agtcacacct tatcaatggt gggctgtacg
    29941 ggggcggagc aatccgcgcc ggtttcttca gtgtccggtt acgccaaccc tgtacagtcc
    30001 gccaccagcg aaattggcgt ttccggtggc ggttatctta accacactga ggaggctgcc
    30061 aacatggcta ctatccctac ccttgtacat tctcaaaccg cctttctctg gcgctttatc
    30121 atctttggcg cgtcagaaca tcaaatcatc cacgtaaccg cctggacgga acgcgaagcg
    30181 cgtagccgtt gcccgtccgg ttgtgttgct gtattcgccg cccgtattcg tcaggaggtg
    30241 tgccatgtgc aataaaacca caccggacgc agcagccgcc gcgctcacta cgctgatgca
    30301 cgcgcttatc gatattgaat gcactgcaga gcttgcgcag ggagaagaac agaaagaccg
    30361 gacacagttc gccctggaat gtatccgata catcgcaacg cggtcgctga atgacgctaa
    30421 aaatattctt gttgctgatt gtgaaaatgg ggggggttat gcgtgatgat cgttttaatt
    30481 ccctgaaaca
//
LOCUS       DAZKHF020000025        30209 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2201S31241
            SAMN43267800-rid25106413.denovo.025, whole genome shotgun sequence.
ACCESSION   DAZKHF020000025 DAZKHF020000000
VERSION     DAZKHF020000025.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267800
            Sequence Read Archive: SRR30307263
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 30209)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 30209)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 30209)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350581.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350581.2
            Genome Coverage       :: 151x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:21:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,652
            CDSs (total)                      :: 5,557
            Genes (coding)                    :: 5,320
            CDSs (with protein)               :: 5,320
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 237
            CDSs (without protein)            :: 237
            Pseudo Genes (ambiguous residues) :: 0 of 237
            Pseudo Genes (frameshifted)       :: 102 of 237
            Pseudo Genes (incomplete)         :: 130 of 237
            Pseudo Genes (internal stop)      :: 52 of 237
            Pseudo Genes (multiple problems)  :: 41 of 237
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..30209
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267800-rid25106413.denovo.025"
                     /serotype="O 26:H11"
                     /isolate="2201S31241"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-01"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            complement(11..259)
                     /gene="hybG"
                     /locus_tag="ACHOSC_RS04810"
                     /old_locus_tag="pgaptmp_000962"
     CDS             complement(11..259)
                     /gene="hybG"
                     /locus_tag="ACHOSC_RS04810"
                     /old_locus_tag="pgaptmp_000962"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase maturation factor HybG"
                     /protein_id="HGU4546946.1"
                     /translation="MCIGVPGQVLAVGEDIHQLAQVEVCGIKRDVNIALICEGNPADL
                     LGQWVLVHVGFAMSIIDEDEAKATLDALRQMDYDITSA"
     gene            complement(272..613)
                     /gene="hypA"
                     /locus_tag="ACHOSC_RS04815"
                     /old_locus_tag="pgaptmp_000963"
     CDS             complement(272..613)
                     /gene="hypA"
                     /locus_tag="ACHOSC_RS04815"
                     /old_locus_tag="pgaptmp_000963"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008901 - ferredoxin hydrogenase activity
                     [Evidence IEA];
                     GO_function: GO:0016151 - nickel cation binding [Evidence
                     IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase maturation nickel metallochaperone
                     HypA"
                     /protein_id="HGU4546947.1"
                     /translation="MHELSLCQSAVEIIQRQAEQHDVKRVTAVWLEIGALSCVEESAV
                     RFSFEIVCHGTVAQGCDLHIVYKPAQAWCWDCSQVVEIHQHDAQCPLCHGERLRVDTG
                     DSLIVKSIEVE"
     gene            complement(606..1094)
                     /gene="hybE"
                     /locus_tag="ACHOSC_RS04820"
                     /old_locus_tag="pgaptmp_000964"
     CDS             complement(606..1094)
                     /gene="hybE"
                     /locus_tag="ACHOSC_RS04820"
                     /old_locus_tag="pgaptmp_000964"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase-2 assembly chaperone"
                     /protein_id="HGU4546948.1"
                     /translation="MTEEIAGFQTSPKAQVQAAFEEIARRSMHDLSFLHPSMPVYVSD
                     FTLFEGQWTGCVITPWMLSAVIFPGPDQLWPLRKVSEKIGLQLPYGTMTFTVGELDGV
                     SQYLSCSLMSPLSHSMSIEEGQRLTDDCARMILSLSVTNPDVPHAGRRALLFGRRSGE
                     NA"
     gene            complement(1087..1581)
                     /locus_tag="ACHOSC_RS04825"
                     /old_locus_tag="pgaptmp_000965"
     CDS             complement(1087..1581)
                     /locus_tag="ACHOSC_RS04825"
                     /old_locus_tag="pgaptmp_000965"
                     /EC_number="3.4.23.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HyaD/HybD family hydrogenase maturation
                     endopeptidase"
                     /protein_id="HGU4546949.1"
                     /translation="MRILVLGVGNILLTDEAIGVRIVEALEQRYILPDYVEILDGGTA
                     GMELLGDMANRDHLIIADAIVSKKNTPGTMMILRDEEVPALFTNKISPHQLGLADVLS
                     ALRFTGEFPKKLTLVGVIPESLEPHIGLTPTVEAMIEPALEQVLAALRESGVEAIPRE
                     AIHD"
     gene            complement(1581..3284)
                     /gene="hybC"
                     /locus_tag="ACHOSC_RS04830"
                     /old_locus_tag="pgaptmp_000966"
     CDS             complement(1581..3284)
                     /gene="hybC"
                     /locus_tag="ACHOSC_RS04830"
                     /old_locus_tag="pgaptmp_000966"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008901 - ferredoxin hydrogenase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 2 large subunit"
                     /protein_id="HGU4546950.1"
                     /translation="MSQRITIDPVTRIEGHLRIDCEIENGVVSKAWASGTMWRGMEEI
                     VKNRDPRDAWMIVQRICGVCTTTHALSSVRAAESALNIDVPVNAQYIRNIILAAHTTH
                     DHIVHFYQLSALDWVDITSALQADPTKASEMLKGVSTWHLNSPEEFTKVQNKIKDLVA
                     SGQLGIFANGYWGHPAMKLPPEVNLIAVAHYLQALECQRDANRVVALLGGKTPHIQNL
                     AVGGVANPINLDGLGVLNLERLMYIKSFIDKLSDFVEQVYKVDTAVIAAFYPEWLTRG
                     KGAVNYLSVPEFPTDSKNGSFLFPGGYIENADLSSYRPITSHSDEYLIKGIQESAKHS
                     WYKDEAPQAPWEGTTIPAYDGWSDDGKYSWVKSPTFYGKTVEVGPLANMLVKLAAGRE
                     STQNKLNEIVAIYQKLTGNTLEVAQLHSTLGRIIGRTVHCCELQDILQNQYSALITNI
                     GKGDHTTFVKPNIPATGEFKGVGFLEAPRGMLSHWMVIKDGIISNYQAVVPSTWNSGP
                     RNFNDDVGPYEQSLVGTPVADPNKPLEVVRTIHSFDPCMACAVHVVDADGNEVVSVKV
                     L"
     gene            complement(3281..4459)
                     /gene="hybB"
                     /locus_tag="ACHOSC_RS04835"
                     /old_locus_tag="pgaptmp_000967"
     CDS             complement(3281..4459)
                     /gene="hybB"
                     /locus_tag="ACHOSC_RS04835"
                     /old_locus_tag="pgaptmp_000967"
                     /EC_number="1.12.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ni/Fe-hydrogenase cytochrome b subunit"
                     /protein_id="HGU4546951.1"
                     /translation="MSHDPQPLGGKIISKPVMIFGPLIVICMLLIVKRLVFGLGSVSD
                     LNGGFPWGVWIAFDLLIGTGFACGGWALAWAVYVFNRGQYHPLVRPALLASLFGYSLG
                     GLSITIDVGRYWNLPYFYIPGHFNVNSVLFETAVCMTIYIGVMALEFAPALFERLGWK
                     VSLKRLNKVMFFIIALGALLPTMHQSSMGSLMISAGYKVHPLWQSYEMLPLFSLLTAF
                     IMGFSIVIFEGSLVQAGLRGNGPDEKSLFVKLTNTISVLLAIFIVLRFGELIYRDKLS
                     LAFAGDFYSVMFWIEVLLMLFPLVVLRVAKLRNDSRMLFLSALSALLGCATWRLTYSL
                     VAFNPGGGYAYFPTWEELLISIGFVAIEICAYIVLIRLLPILPPLKQNDHNRHEASKA
                     "
     gene            complement(4449..5435)
                     /gene="hybA"
                     /locus_tag="ACHOSC_RS04840"
                     /old_locus_tag="pgaptmp_000968"
     CDS             complement(4449..5435)
                     /gene="hybA"
                     /locus_tag="ACHOSC_RS04840"
                     /old_locus_tag="pgaptmp_000968"
                     /EC_number="1.12.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 2 operon protein HybA"
                     /protein_id="HGU4546952.1"
                     /translation="MNRRNFIKAASCGALLTGALPSVSHAAAENRPPIPGSLGMLYDS
                     TLCVGCQACVTKCQDINFPERNPQGEQTWSNNDKLSPYTNNIIQVWTSGTGVNKDQEE
                     NGYAYIKKQCMHCVDPNCVSVCPVSALKKDPKTGIVHYDKDVCTGCRYCMVACPYNVP
                     KYDYNNPFGALHKCELCNQKGVERLDKGGLPGCVEVCPAGAVIFGTREELMAEAKKRL
                     ALKPGSEYHYPRQTLKSGDTYLHTVPKYYPHLYGEKEGGGTQVLVLTGVPYENLDLPK
                     LDDLSTGARSENIQHTLYKGMMLPLAVLAGLTVLVRRNTKNDHHDGGDDHES"
     gene            complement(5438..6556)
                     /gene="hybO"
                     /locus_tag="ACHOSC_RS04845"
                     /old_locus_tag="pgaptmp_000969"
     CDS             complement(5438..6556)
                     /gene="hybO"
                     /locus_tag="ACHOSC_RS04845"
                     /old_locus_tag="pgaptmp_000969"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 2 small subunit"
                     /protein_id="HGU4546953.1"
                     /translation="MTGDNTLIHSHGINRRDFMKLCAALAATMGLSSKAAAEMAESVT
                     NPQRPPVIWIGAQECTGCTESLLRATHPTVENLVLETISLEYHEVLSAAFGHQVEENK
                     HNALEKYKGQYVLVVDGSIPLKDNGIYCMVAGEPIVDHIRKAAEGAAAIIAIGSCSAW
                     GGVAAAGVNPTGAVSLQEVLPGKTVINIPGCPPNPHNFLATVAHIITYGKPPKLDDKN
                     RPTFAYGRLIHEHCERRPHFDAGRFAKEFGDEGHREGWCLYHLGCKGPETYGNCSTLQ
                     FCDVGGVWPVAIGHPCYGCNEEGIGFHKGIHQLANVENQTPRSQKPDVNAKEGGNVSA
                     GAIGLLGGVVGLVAGVSVMAVRELGRQQKKDNADSRGE"
     gene            complement(6745..7032)
                     /gene="yghW"
                     /locus_tag="ACHOSC_RS04850"
                     /old_locus_tag="pgaptmp_000970"
     CDS             complement(6745..7032)
                     /gene="yghW"
                     /locus_tag="ACHOSC_RS04850"
                     /old_locus_tag="pgaptmp_000970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2623 family protein YghW"
                     /protein_id="HGU4546954.1"
                     /translation="MNNHFGKGLMAGLKATHADSAVNVTKFCADYKRGFVLGYSHRMY
                     EKTGDRQLSAWEAGILTRRYGLDKEMVMDFFRENNSCSTLRFFMAGYRLEN"
     gene            complement(7151..8038)
                     /gene="yghX"
                     /locus_tag="ACHOSC_RS04855"
                     /old_locus_tag="pgaptmp_000971"
     CDS             complement(7151..8038)
                     /gene="yghX"
                     /locus_tag="ACHOSC_RS04855"
                     /old_locus_tag="pgaptmp_000971"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311911.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YghX family hydrolase"
                     /protein_id="HGU4546955.1"
                     /translation="MPRLTAKDFPQELLDYYDYYAHGKISKREFLNLAAKYAVGGMTA
                     LALFDLLKPNYALATQVEFTDPEIVAEYITYPSPNGHGEVRGYLVKPAKMSGKTPAVV
                     VVHENRGLNPYIEDVARRVAKAGYIALAPDGLSSVGGYPGNDDKGRELQQQVDPTKLM
                     NDFFAAIEFMQRYPQAAGKVGITGFCYGGGVSNAAAVAYPELACAVPFYGRQAPTADV
                     AKIEAPLLLHFAELDTRINEGWPAYEAALKANNKVYEAYIYPGVNHGFHNDSTPRYDK
                     SAADLAWQRTLKWFDKYLS"
     gene            8195..9235
                     /gene="gpr"
                     /locus_tag="ACHOSC_RS04860"
                     /old_locus_tag="pgaptmp_000972"
     CDS             8195..9235
                     /gene="gpr"
                     /locus_tag="ACHOSC_RS04860"
                     /old_locus_tag="pgaptmp_000972"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-glyceraldehyde 3-phosphate reductase"
                     /protein_id="HGU4546956.1"
                     /translation="MVWLANPERYGQMQYRYCGKSGLRLPALSLGLWHNFGHVNALES
                     QRAILRKAFDLGITHFDLANNYGPPPGSAEENFGRLLREDFAAYRDELIISTKAGYDM
                     WPGPYGSGGSRKYLLASLDQSLKRMGLEYVDIFYSHRVDENTPMEETASALAHAVQSG
                     KALYVGISSYSPERTQKMVELLHEWKIPLLIHQPSYNLLNRWVDKSGLLDTLQNNGVG
                     CIAFTPLAQGLLTGKYLNGIPQDSRMYREGNKVRGLTPKMLTEANLNSLRLLNEMAQQ
                     RGQSMAQMALSWLLKDDRVTSVLIGASRAEQLEENVQALNNLTFSTEELAQIDQHIAD
                     GELNLWQASSDK"
     gene            complement(9275..9769)
                     /locus_tag="ACHOSC_RS04865"
                     /old_locus_tag="pgaptmp_000973"
     CDS             complement(9275..9769)
                     /locus_tag="ACHOSC_RS04865"
                     /old_locus_tag="pgaptmp_000973"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00645 family protein"
                     /protein_id="HGU4546957.1"
                     /translation="MERFLENAMYASRWLLAPVYFGLSLALVALALKFFQEIINVLPN
                     IFSMAESDLILVLLSLVDMTLVGGLLVMVMFSGYENFVSQLDISENKEKLNWLGKMDA
                     TSLKNKVAASIVAISSIHLLRVFMDAKNVPDNKLMWYVIIHLTFVLSAFVMGYLDRLT
                     RHNH"
     gene            9960..10844
                     /gene="yghA"
                     /locus_tag="ACHOSC_RS04870"
                     /old_locus_tag="pgaptmp_000974"
     CDS             9960..10844
                     /gene="yghA"
                     /locus_tag="ACHOSC_RS04870"
                     /old_locus_tag="pgaptmp_000974"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP(+)-dependent aldehyde reductase"
                     /protein_id="HGU4546958.1"
                     /translation="MSHLKDPTTQYYTGEYPKQKQPTPGIQAKMTPVPDCGEKTYVGS
                     GRLKDRKALVTGGDSGIGRAAAIAYAREGADVAISYLPVEEEDAQDVKKIIEECGRKA
                     VLLPGDLSDEKFARSLVHEAHKALGGLDIMALVAGKQVAIPDIADLTSEQFQKTFAIN
                     VFALFWLTQEAIPLLPKGASIITTSSIQAYQPSPHLLDYAATKAAILNYSRGLAKQVA
                     EKGIRVNIVAPGPIWTALQISGGQTQDKIPQFGQKTPMKRAGQPAELAPVYVYLASQE
                     SSYVTAEVHGVCGGEHLG"
     gene            complement(11116..11541)
                     /gene="exbD"
                     /locus_tag="ACHOSC_RS04875"
                     /old_locus_tag="pgaptmp_000975"
     CDS             complement(11116..11541)
                     /gene="exbD"
                     /locus_tag="ACHOSC_RS04875"
                     /old_locus_tag="pgaptmp_000975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB system transport protein ExbD"
                     /protein_id="HGU4546959.1"
                     /translation="MAMHLNENLDDNGEMHDINVTPFIDVMLVLLIIFMVAAPLATVD
                     VKVNLPASTSTPQPRPEKPVYLSVKADNSMFIGNDPVTDETMITALNALTEGKKDTTI
                     FFRADKTVDYETLMKVMDTLHQAGYLKIGLVGEETAKAK"
     gene            complement(11548..12282)
                     /gene="exbB"
                     /locus_tag="ACHOSC_RS04880"
                     /old_locus_tag="pgaptmp_000976"
     CDS             complement(11548..12282)
                     /gene="exbB"
                     /locus_tag="ACHOSC_RS04880"
                     /old_locus_tag="pgaptmp_000976"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tol-pal system-associated acyl-CoA thioesterase"
                     /protein_id="HGU4546960.1"
                     /translation="MGNNLMQTDLSVWGMYQHADIVVKCVMIGLILASVVTWAIFFSK
                     SVEFFNQKRRLKREQQLLAEARSLNQANDIAADFGSKSLSLHLLNEAQNELELSEGSD
                     DNEGIKERTSFRLERRVAAVGRQMGRGNGYLATIGAISPFVGLFGTVWGIMNSFIGIA
                     QTQTTNLAVVAPGIAEALLATAIGLVAAIPAVVIYNVFARQIGGFKAMLGDVAAQVLL
                     LQSRDLDLEASAAAHPVRVAQKLRAG"
     gene            12534..13721
                     /gene="metC"
                     /locus_tag="ACHOSC_RS04885"
                     /old_locus_tag="pgaptmp_000977"
     CDS             12534..13721
                     /gene="metC"
                     /locus_tag="ACHOSC_RS04885"
                     /old_locus_tag="pgaptmp_000977"
                     /EC_number="4.4.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_function: GO:0047804 - cysteine-S-conjugate beta-lyase
                     activity [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA];
                     GO_process: GO:0019346 - transsulfuration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cystathionine beta-lyase"
                     /protein_id="HGU4546961.1"
                     /translation="MADKKLDTQLVNAGRSKKYTLGAVNSVIQRASSLVFDSVEAKKH
                     ATRNRANGELFYGRRGTLTHFSLQQAMCELEGGAGCALFPCGAAAVANSILAFVEQGD
                     HVLMTNTAYEPSQDFCSKILSKLGVTTSWFDPLIGADIVKHLQPNTKIVFLESPGSIT
                     MEVHDVPAIVAAVRSVVPDAIIMIDNTWAAGVLFKALDFGIDVSIQAATKYLVGHSDA
                     MIGTAVCNARCWEQLRENAYLMGQMVDADTAYITSRGLRTLGVRLRQHHESSLKVAEW
                     LAEHPQVARVNHPALPGSKGHEFWKRDFTGSSGLFSFVLKKKLNNEELANYLDNFSLF
                     SMAYSWGGYESLILANQPEHIAAIRPQGEIDFSGTLIRLHIGLEDVDDLIADLDAGFA
                     RIV"
     gene            13861..14520
                     /gene="yghB"
                     /locus_tag="ACHOSC_RS04890"
                     /old_locus_tag="pgaptmp_000978"
     CDS             13861..14520
                     /gene="yghB"
                     /locus_tag="ACHOSC_RS04890"
                     /old_locus_tag="pgaptmp_000978"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family general envelope maintenance protein
                     YghB"
                     /protein_id="HGU4546962.1"
                     /translation="MAVIQDIIAALWQHDFAALADPHIVSVVYFVMFATLFLENGLLP
                     ASFLPGDSLLILAGALIAQGVMDFLPTIAILTAAASLGCWLSYIQGRWLGNTKTVKGW
                     LAQLPAKYHQRATCMFDRHGLLALLAGRFLAFVRTLLPTMAGISGLPNRRFQFFNWLS
                     GLLWVSVVTSFGYALSMIPFVKRHEDQVMTFLMILPIALLTAGLLGTLFVVIKKKYCN
                     A"
     gene            complement(14560..15459)
                     /gene="yqhC"
                     /locus_tag="ACHOSC_RS04895"
                     /old_locus_tag="pgaptmp_000979"
     CDS             complement(14560..15459)
                     /gene="yqhC"
                     /locus_tag="ACHOSC_RS04895"
                     /old_locus_tag="pgaptmp_000979"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001407451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator YqhC"
                     /protein_id="HGU4546963.1"
                     /translation="MKREEICRLLADKVNKLKIKENSLSELLPDVRLLYGETPFARTP
                     VMYEPGIIILFSGHKIGYINERVFRYDANEYLLLTVPLPFECETYATSEVPLAGLRLN
                     VDILQLQELLMDIGEDEHFQPSMAASGINSATLSEEILCAAERLLDVMERPLDARILG
                     KQIIREILYYVLTGPCGGALLALVSRQTHFSLISRVLKRIENKYTENLSVEQLAAEAN
                     MSVSAFHHNFKSVTSTSPLQYLKNYRLHKARMMIIHDGMKASAAAMRVGYESASQFSR
                     EFKRYFGVTPGEDAARMRAMQGN"
     gene            15653..16816
                     /gene="yqhD"
                     /locus_tag="ACHOSC_RS04900"
                     /old_locus_tag="pgaptmp_000980"
     CDS             15653..16816
                     /gene="yqhD"
                     /locus_tag="ACHOSC_RS04900"
                     /old_locus_tag="pgaptmp_000980"
                     /EC_number="1.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:1990362 - butanol dehydrogenase (NAD+)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase"
                     /protein_id="HGU4546964.1"
                     /translation="MNNFNLHTPTRILFGKGAIAGLREQIPHDARVLITYGGGSVKKT
                     GVLDQVLDALKGMDVLEFGGIEPNPAYETLMNAVKLVREQKVTFLLAVGGGSVLDGTK
                     FIAAAANYPENIDPWHILQTGGKEIKSAIPMGCVLTLPATGSESNAGAVISRKTTGDK
                     QAFHSAHVQPVFAVLDPVYTYTLPPRQVANGVVDAFVHTVEQYVTKPVDAKIQDRFAE
                     GILLTLIEDGPKALKEPENYDVRANVMWAATQALNGLIGAGVPQDWATHMLGHELTAM
                     HGLDHAQTLAIVLPALWNEKRETKRAKLLQYAERVWNITEGSDDERIDTAIAATRNFF
                     EQLGVPTHLSDYGLDGSSIPALLKKLEEHGMTQLGENHDITLDVSRRIYEAAR"
     gene            16921..17748
                     /gene="dkgA"
                     /locus_tag="ACHOSC_RS04905"
                     /old_locus_tag="pgaptmp_000981"
     CDS             16921..17748
                     /gene="dkgA"
                     /locus_tag="ACHOSC_RS04905"
                     /old_locus_tag="pgaptmp_000981"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417485.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050580 - 2,5-didehydrogluconate reductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2,5-didehydrogluconate reductase DkgA"
                     /protein_id="HGU4546965.1"
                     /translation="MANPTVIKLQDGNVMPQLGLGVWQASNEEVITAIQKALEVGYRS
                     IDTAAAYKNEEGVGKALKNASVNREELFITTKLWNDDHKRPREALLDSLKKLQLDYID
                     LYLMHWPVPAIDHYVEAWKGMIELQKEGLIKSIGVCNFQIHHLQRLIDETGVTPVINQ
                     IELHPLMQQRQLHAWNATHKIQTESWSPLAQGGKGVFDQKVIRDLADKYGKTPAQIVI
                     RWHLDSGLVVIPKSVTPSRIAENFDVWDFRLDKDELGEIAKLDQGKRLGPDPDQFGG"
     gene            17948..18874
                     /locus_tag="ACHOSC_RS04910"
                     /old_locus_tag="pgaptmp_000982"
     CDS             17948..18874
                     /locus_tag="ACHOSC_RS04910"
                     /old_locus_tag="pgaptmp_000982"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbjP/YqhG family protein"
                     /protein_id="HGU4546966.1"
                     /translation="MKIILLFLAALASFTVHAQPPSLTVEQTVRHIYQNYKSDATAPY
                     FGETGERAITSARIQQALTLNDNLTLPGNIGWLDYDPVCDCQDFGDLVLESVAITQTD
                     ADHADAVVRFRIFKDDKEKTTQTLKMVAENGRWVIDDIVSNHGSVLQAVNSENEKTLA
                     ALASLQKEQPEAFVAELFEHIADYSWPWTWVVSDSYRQAVNAFYKTTFKTANNPDEDM
                     QIERQFIYDNPICFGEESLFSRVDEIRVLEKTADSARIHVRFTLTNGNNEEQELVLQR
                     REGKWEIADFIRPNSGSLLKQIEAKTAARLKQ"
     gene            18925..19182
                     /gene="yqhH"
                     /locus_tag="ACHOSC_RS04915"
                     /old_locus_tag="pgaptmp_000983"
     CDS             18925..19182
                     /gene="yqhH"
                     /locus_tag="ACHOSC_RS04915"
                     /old_locus_tag="pgaptmp_000983"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein YqhH"
                     /protein_id="HGU4546967.1"
                     /translation="MKTIFTVGAVVLATCLLSGCVNEQKVNQLASNVQTLNAKIARLE
                     QDMKALRPQIYAAKSEANRANTRLDAQDYFDCLRCLRMYAE"
     gene            complement(19225..21444)
                     /locus_tag="ACHOSC_RS04920"
                     /old_locus_tag="pgaptmp_000984"
     CDS             complement(19225..21444)
                     /locus_tag="ACHOSC_RS04920"
                     /old_locus_tag="pgaptmp_000984"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YgiQ family radical SAM protein"
                     /protein_id="HGU4546968.1"
                     /translation="MSSISLIQPDRDLFSWPQYWAACFGPAPFLPMSREEMDQLGWDS
                     CDIILVTGDAYVDHPSFGMAICGRMLEAQGFRVGIIAQPDWSSKDDFMRLGKPNLFFG
                     VTAGNMDSMINRYTADRRLRHDDAYTPDNVAGKRPDRATLVYTQRCKEAWKDVPVILG
                     GIEASLRRTAHYDYWSDTVRRSVLVDSKADMLMFGNGERPLVEVAHRLAMGEPISEIR
                     DVRNTAIIVKEALPGWSGVDSTRLDTPGKIDPIPHPYGEDLPCADNKPVAPKKQEAKA
                     VTVQPPRPKPWEKTYVLLPSFEKVKGDKVLYAHASRILHHETNPGCARALMQKHGDRY
                     VWINPPAIPLSTEEMDSVFALPYKRVPHPAYGNARIPAYEMIRFSVNIMRGCFGGCSF
                     CSITEHEGRIIQSRSEDSIINEIEAIRDTVPGFTGVISDLGGPTANMYMLRCKSPRAE
                     QTCRRLSCVYPDICPHMDTNHEPTINLYRRARDLKGIKKILIASGVRYDIAVEDPRYI
                     KELATHHVGGYLKIAPEHTEEGPLSKMMKPGMGSYDRFKELFDTYSKQAGKEQYLIPY
                     FISAHPGTRDEDMVNLALWLKKHRFRLDQVQNFYPSPLANSTTMYYTGKNPLAKIGYK
                     SEDVFVPKGDKQRRLHKALLRYHDPANWPLIRQALEAMGKKHLIGSRRDCLVPAPTIE
                     EMREARRQNRNTRPALTKHTPMATQRQTPATAKKASSTQSRPVNAGAKKRPKAAVGR"
     gene            complement(21555..22967)
                     /gene="ftsP"
                     /locus_tag="ACHOSC_RS04925"
                     /old_locus_tag="pgaptmp_000985"
     CDS             complement(21555..22967)
                     /gene="ftsP"
                     /locus_tag="ACHOSC_RS04925"
                     /old_locus_tag="pgaptmp_000985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0043093 - FtsZ-dependent cytokinesis
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsP"
                     /protein_id="HGU4546969.1"
                     /translation="MSLSRRQFIQASGIALCAGAVPLKASAAGQQQPLPVPPLLESRR
                     GQPLFMTVQRAHWSFTPGTRASVWGINGRYLGPTIRVWKGDDVKLIYSNRLTENVSMT
                     VAGLQVPGPLMGGPARMMSPNADWAPVLPIRQNAATLWYHANTPNRTAQQVYNGLAGM
                     WLVEDEVSKSLPIPNHYGVDDFPVIIQDKRLDNFGTPEYNEPGSGGFVGDTLLVNGVQ
                     SPYVEVSRGWVRLRLLNASNSRRYQLQMSDGRPLHVISGDQGFLPAPVSVKQLSLAPG
                     ERREILVDMSNGDEVSITCGEAASIVDRIRGFFEPSSILVSTLVLTLRPTGLLPLVTD
                     SLPMRLLPTEIMAGSPIRSRDISLGDDPGINGQLWDVNRIDVTAQQGTWERWTVRADE
                     PQAFHIEGVMFQIRNVNGAMPFPEDRGWKDTVWVDGQVELLVYFGQPSWAHFPFYFNS
                     QTLEMADRGSIGQLLVNPVP"
     gene            complement(23042..23779)
                     /gene="plsC"
                     /locus_tag="ACHOSC_RS04930"
                     /old_locus_tag="pgaptmp_000986"
                     /gene_synonym="parF"
     CDS             complement(23042..23779)
                     /gene="plsC"
                     /locus_tag="ACHOSC_RS04930"
                     /old_locus_tag="pgaptmp_000986"
                     /gene_synonym="parF"
                     /EC_number="2.3.1.51"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-acylglycerol-3-phosphate O-acyltransferase"
                     /protein_id="HGU4546970.1"
                     /translation="MLYIFRLIITVIYSILVCVFGSIYCLFSPRNPKHVATFGHMFGR
                     LAPLFGLKVECRKPADAESYGNAIYIANHQNNYDMVTASNIVQPPTVTVGKKSLLWIP
                     FFGQLYWLTGNLLIDRNNRTKAHGTIAEVVNHFKKRRISIWMFPEGTRSRGRGLLPFK
                     TGAFHAAIAAGVPIIPVCVSTTSNKINLNRLHNGLVIVEMLPPIDVSQYGKDQVRELA
                     AHCRSIMEQKIAELDKEVAEREAAGKV"
     gene            complement(24012..26270)
                     /gene="parC"
                     /locus_tag="ACHOSC_RS04935"
                     /old_locus_tag="pgaptmp_000987"
     CDS             complement(24012..26270)
                     /gene="parC"
                     /locus_tag="ACHOSC_RS04935"
                     /old_locus_tag="pgaptmp_000987"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009340 - DNA topoisomerase IV complex
                     [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IV subunit A"
                     /protein_id="HGU4546971.1"
                     /translation="MSDMAERLALHEFTENAYLNYSMYVIMDRALPFIGDGLKPVQRR
                     IVYAMSELGLNASAKFKKSARTVGDVLGKYHPHGDSACYEAMVLMAQPFSYRYPLVDG
                     QGNWGAPDDPKSFAAMRYTESRLSKYSELLLSELGQGTADWVPNFDGTLQEPKMLPAR
                     LPNILLNGTTGIAVGMATDIPPHNLREVAQAAIALIDQPKTTLEQLLDIVQGPDYPTE
                     AEIITSRAEIRKIYENGRGSVRMRAVWKKEDGAVVISALPHQVSGARVLEQIAAQMRN
                     KKLPMVDDLRDESDHENPTRLVIVPRSNRVDMDQVMNHLFATTDLEKSYRINLNMIGL
                     DGRPAVKNLLEILSEWLVFRRDTVRRRLNYRLEKVLKRLHILEGLLVAFLNIDEVIEI
                     IRNEDEPKPALMSRFGLTETQAEAILELKLRHLAKLEEMKIRGEQSELEKERDQLQGI
                     LASERKMNNLLKKELQADAQAYGDDRRSPLQEREEAKAMSEHDMLPSEPVTIVLSQMG
                     WVRSAKGHDIDAPGLNYKAGDSFKAAVKGKSNQPVVFVDSTGRSYAIDPITLPSARGQ
                     GEPLTGKLTLPPGATVDHMLMESDDQKLLMASDAGYGFVCTFNDLVARNRAGKALITL
                     PENAHVMPPVVIEDASDMLLAITQAGRMLMFPVSDLPQLSKGKGNKIINIPSAEAARG
                     EDGLAQLYVLPPQSTLTIHVGKRKIKLRPEELQKVTGERGRRGTLMRGLQRIDRVEID
                     SPRRASSGDSEE"
     gene            complement(26816..27298)
                     /locus_tag="ACHOSC_RS04940"
                     /old_locus_tag="pgaptmp_000988"
     CDS             complement(26816..27298)
                     /locus_tag="ACHOSC_RS04940"
                     /old_locus_tag="pgaptmp_000988"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GyrI-like domain-containing protein"
                     /protein_id="HGU4546972.1"
                     /translation="MTNLTLDVNIIDFPSIPVAMLPHRCSPELLNYSVAKFIMWRKET
                     GLSPVNQSQTFGVAWDDPATTAPEAFRFDICGSVSEPIPDNRYGVSNGELTGGRYAVA
                     RHVGELDDISHTIWGIIRHWLPASGEKMRKAPIMFHYTNLAEGVTEQRLETDVYVPLA
                     "
     gene            complement(27351..27743)
                     /gene="ygiW"
                     /locus_tag="ACHOSC_RS04945"
                     /old_locus_tag="pgaptmp_000989"
     CDS             complement(27351..27743)
                     /gene="ygiW"
                     /locus_tag="ACHOSC_RS04945"
                     /old_locus_tag="pgaptmp_000989"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OB fold stress tolerance protein YgiW"
                     /protein_id="HGU4546973.1"
                     /translation="MKKFAAVIAVMALCSAPVMAAEQGGFSGPSATQSQAGGFQGPNG
                     SVTTVESAKSLRDDTWVTLRGNIVERISDDLYVFKDASGTINVDIDHKRWNGVTVTPK
                     DTVEIQGEVDKDWNSVEIDVKQIRKVNP"
     gene            27895..28554
                     /gene="qseB"
                     /locus_tag="ACHOSC_RS04950"
                     /old_locus_tag="pgaptmp_000990"
     CDS             27895..28554
                     /gene="qseB"
                     /locus_tag="ACHOSC_RS04950"
                     /old_locus_tag="pgaptmp_000990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quorum sensing response regulator transcription
                     factor QseB"
                     /protein_id="HGU4546974.1"
                     /translation="MRILLIEDDMLIGDGIKTGLSKMGFSVDWFTQGRQGKEALYSAP
                     YDAVILDLTLPGMDGRDILREWREKGQREPVLILTARDALEERVEGLRLGADDYLCKP
                     FALIEVAARLEALMRRTNGQASNELRHGNVMLDPGKRIATLAGEPLTLKPKEFALLEL
                     LMRNAGRVLPRKLIEEKLYTWDEEVTSNAVEVHVHHLRRKLGSDFIRTVHGIGYTLGE
                     K"
     gene            28551..29900
                     /gene="qseC"
                     /locus_tag="ACHOSC_RS04955"
                     /old_locus_tag="pgaptmp_000991"
     CDS             28551..29900
                     /gene="qseC"
                     /locus_tag="ACHOSC_RS04955"
                     /old_locus_tag="pgaptmp_000991"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_306641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="quorum sensing histidine kinase QseC"
                     /protein_id="HGU4546975.1"
                     /translation="MKFTQRLSLRVRLTLIFLILASVTWLLSSFVAWKQTTDNVDELF
                     DTQLMLFAKRLSTLDLNEINAADRMAQTPNKLKHGHVDDDALTFAIFTHDGRMVLNDG
                     DNGEDIPYSYQREGFADGQLVGDKDQWRFVWMTSPDGKYRIVVGQEWEYREDMALAIV
                     AGQLIPWLVALPIMLIIMMVLLGRELAPLNKLALALRMRDPDSEKPLNATGVPSEVRP
                     LVESLNQLFARTHAMMVRERRFTSDAAHELRSPLTALKVQTEVAQLSDDDPQARKKAL
                     LQLHYGIDRATRLVDQLLTLSRLDSLDNLQDVAEIPLEDLLQSSVMDIYHTAQQAKID
                     VRLTLNAHGIKRTGQPLLLSLLVRNLLDNAVRYSPQGSVIDVTLNADNFIVRDNGPGV
                     TPEALARIGERFYRPPGQTATGSGLGLSIVQRIAKLHGMNVEFGNAEQGGFEAKVSW"
     gene            complement(29946..>30209)
                     /locus_tag="ACHOSC_RS04960"
                     /old_locus_tag="pgaptmp_000992"
     CDS             complement(29946..>30209)
                     /locus_tag="ACHOSC_RS04960"
                     /old_locus_tag="pgaptmp_000992"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2645 family protein"
                     /protein_id="HGU4546976.1"
                     /translation="DAFFAFNKYDWMLESGDNICSIPSVSGEDRILQAMIAAFFLLTP
                     LIILILRKLFMREMFEFWVYVFSLGICLVCGWWLFWGRFIFCY"
BASE COUNT         7457 a         8076 c         7629 g         7047 t
ORIGIN      
        1 aagctaatca tcacgcgctg gtaatgtcgt aatccatttg gcgcagtgcg tctaatgtgg
       61 ctttggcttc atcttcgtcg atgatgctca tggcaaatcc gacgtgtacc agcacccact
      121 ggcccagtag atcggcaggg ttaccttcac aaatcagggc gatattcacg tcgcgcttga
      181 taccacatac ttcgacctgc gcaagctggt gaatatcttc accgacagcc agcacctggc
      241 ctggaacgcc aatacacatt attaactccg gttattcaac ttcaatactt ttgacgatca
      301 gcgaatcgcc ggtatcgaca cgcaaccgct cgccgtgaca gagcggacac tgcgcatcgt
      361 gctgatgaat ctccaccacc tggctgcaat cccagcacca agcctgggcg ggtttataga
      421 cgatatgtaa atcgcaccct tgcgccaccg ttccgtggca gacaatttca aaactaaaac
      481 ggacggcgct ctcctcaacg caggagagcg cgccaatttc cagccacacg gcggtgacgc
      541 gcttaacatc gtgctgctcc gcctgccgtt ggataatttc aacggcgctc tggcaaagag
      601 acaactcatg cattttcgcc actcctgcga ccaaacagca gggcgcgacg ccctgcgtgt
      661 ggtacatccg gattcgtgac tgacagcgaa aggatcattc gtgcgcagtc atccgtcagg
      721 cgttggccct cttcaatcga catgctgtgc gaaagcggcg acatcagcga acaggagaga
      781 tattgcgaaa caccgtccag ttcgccaacg gtaaaggtca tagtgccata cggcagttgc
      841 agaccaattt tttcactcac tttgcgcagc ggccagagtt gatccgggcc ggggaagata
      901 actgcactca gcatccacgg ggtgatcaca caccccgtcc actgaccttc gaacagcgta
      961 aaatcagaaa catacaccgg cattgaagga tgcagaaaag agagatcgtg catcgaacgc
     1021 cgggcaattt cttcaaacgc tgcctgtact tgcgccttcg gggaggtctg gaaacctgct
     1081 atctcttcag tcatgaatcg cctcccgtgg gatggcttcc acgccagatt cacgcagcgc
     1141 agccagaacc tgctcaagcg caggttcaat cattgcttca accgtcggcg ttaagccgat
     1201 gtgtggctcc agcgattccg ggatcacgcc gaccagggtc agctttttcg gaaactcacc
     1261 ggtgaagcgc agggccgaca atacgtcggc caggccaagc tgatgcggag agattttgtt
     1321 ggtaaacaac gccggaactt cttcatcccg caggatcatc atcgttcccg gcgtgttctt
     1381 tttcgacaca atggcatccg caataatcag atgatcgcga tttgccatgt cgccaagcag
     1441 ctccattccc gccgtgccgc catcgaggat ctcaacataa tccggcagaa tgtatcgttg
     1501 ctctaacgct tcgacaatcc gcacaccgat ggcttcatcg gtcagcaaaa tattgccgac
     1561 ccctaagact aaaatacgca ttacagaacc ttcactgaaa ccacttcgtt gccgtcggca
     1621 tccactacgt gtaccgcaca ggccatgcac gggtcgaagg agtgaatggt acgcaccact
     1681 tccagcggtt tattcggatc agcaaccggt gtacccacca gcgactgctc gtaaggaccg
     1741 acgtcatcat tgaagttacg cggaccagag ttccaggttg atggaacaac tgcctggtag
     1801 ttgctgatga taccgtcttt aatcaccatc cagtgagaga gcataccgcg cggcgcttcg
     1861 aggaagccaa cacctttgaa ctcacccgtt gccggaatgt tcggtttcac aaaggtggtg
     1921 tgatcgcctt tgccgatatt ggtgatcagt gcactgtatt ggttttgcag gatatcctgc
     1981 aattcacagc agtgaacggt acgaccaata atacggccca gcgtggagtg cagctgtgcc
     2041 acttccagcg tgttgccagt cagtttctga taaatcgcaa cgatttcatt cagtttgttc
     2101 tgggtagatt ctcgacctgc cgccagtttc accagcatat tagccagcgg acccacttct
     2161 accgttttgc cgtagaaagt cggtgatttc acccaggaat atttcccgtc gtcagaccaa
     2221 ccatcataag ccggaatggt ggtaccttcc cacggcgcct gtggcgcttc gtctttatac
     2281 caggagtgct tcgcgctttc ctgaatgcct ttgatcaggt attcatcgga atgagaagtg
     2341 atcggacgat acgaggacag atccgcattc tcaatgtagc cgcccgggaa caggaagctg
     2401 ccgtttttgc tgtcggtcgg gaattccggc acgctcaggt agttcaccgc acctttaccg
     2461 cgcgtcagcc attccgggta gaacgcggcg ataaccgcgg tatcaacctt ataaacctgc
     2521 tcaacaaagt cgctcagttt gtcgatgaaa gacttgatgt acatcaggcg ctcaaggttc
     2581 aacacgccca acccgtcgag gttgattggg ttcgcgacac cacccactgc caggttctga
     2641 atgtgcggcg ttttaccgcc cagcagcgcc acgacgcggt tagcgtcacg ctggcactcc
     2701 agcgcctgca ggtagtgcgc taccgcaatc aggttcactt ccggcggcag tttcatcgct
     2761 gggtgacccc agtagccatt agcgaaaata cccaactgac cgctggcaac cagatctttg
     2821 atcttgttct gaactttagt gaactcttcc ggactgttca gatgccaggt cgaaacgcct
     2881 ttcagcattt cggaggcttt ggttgggtca gcttgcagtg cagaagtgat gtccacccag
     2941 tccagcgccg aaagctgata gaaatgaacg atatggtcat gcgtggtgtg cgcagccaga
     3001 atgatgttac ggatgtattg cgcgttaacc ggaacgtcga tattcagcgc actttctgcc
     3061 gcacgaacgg aagacagcgc gtgagtggta gtacatacgc cacagatacg ttgcacaatc
     3121 atccatgcat cgcgcggatc gcggtttttc acgatctctt ccatgccgcg ccacatggta
     3181 ccggaagccc atgctttcga aacgacgcca ttttcgattt cgcaatcgat gcgtaaatgc
     3241 ccctcaatac gggttaccgg atcaatagta attctctggc tcatgctttg ctcgcctcat
     3301 gacgattatg atcgttttgt tttaaaggag gaagtatcgg cagtagacga atgagtacga
     3361 tgtaagcgca aatctcaata gccacaaaac caatagaaat caacagttct tcccaggtcg
     3421 ggaagtaggc gtaaccgccg cccgggttga atgccaccag cgaataggtc agacgccagg
     3481 ttgcacaacc taacagtgcg ctcagtgctg acaggaacag catgcgggaa tcattacgca
     3541 gcttcgccac acgcagaacc accagcggga agagcatcag caggacttca atccagaaca
     3601 tcacggagta gaagtcaccg gcaaacgcta acgacagctt gtcgcgatag ataagctcgc
     3661 caaagcgcag cacgatgaaa atcgccagca acacactgat ggtgttggtc agcttgacga
     3721 acagactctt ttcatccgga ccgttgccac gcagacccgc ctgcaccagc gaaccttcaa
     3781 agatgacaat cgagaagccc atgatgaacg ccgtcagcag cgagaacagc ggcaacattt
     3841 catagctctg ccacaacgga tgcaccttgt agcccgccga gatcatcagt gaccccattg
     3901 aagactggtg catggtcggc aacagcgcgc caagtgcaat gatgaagaac atcaccttgt
     3961 tcagacgctt gagagaaacc ttccagccca gacgttcaaa cagtgccgga gcaaactcca
     4021 gtgccatcac gccgatatag atggtcatac agaccgccgt ctcgaacagt accgagttca
     4081 cgttgaagtg acccggaatg tagaagtacg gcaggttcca gtagcgaccc acgtcgatag
     4141 tgatcgacaa gccccccagt gagtaaccaa acagactcgc caacagcgcc ggacgcacca
     4201 gcggatggta ttgcccacgg ttaaagacgt ataccgccca cgccagcgcc cagccgccac
     4261 aggcaaagcc ggtaccaatc agcaggtcaa acgcgatcca cacgccccac gggaagccgc
     4321 cgttcagatc agagacagag cccagaccga acaccagacg cttcacaatc aggagcatac
     4381 agatgacgat taacggtcca aaaatcatga ccggtttact gatgattttg ccgcccagcg
     4441 gttgtggatc atgactcatg atcgtctcct ccgtcgtgat ggtcgttttt ggtgttgcga
     4501 cgaaccagca cggttaagcc cgccagcaca gccagtggta gcatcatgcc tttatacagg
     4561 gtgtgttgaa tattttcgga acgcgcaccg gtagaaagat cgtccagttt cggcaggtcg
     4621 agattttcat aaggcacacc cgtcagtacc agaacctgag taccgccgcc ctctttctcg
     4681 ccgtacagat gcggataata tttcggcacc gtatgcaggt aagtgtcgcc agatttcagc
     4741 gtctgacgcg gatagtggta ttcgctgcca ggcttcagcg ccagacgttt tttcgcctcc
     4801 gccatcagct cttcacgcgt accaaaaatt accgcgcccg ccgggcacac ttctacgcag
     4861 ccaggcaaac cgcctttatc gagacgttcc acacctttct ggttgcacaa ctcgcactta
     4921 tgcagcgcac caaacgggtt gttgtagtcg tacttcggca cgttgtatgg gcaggcgacc
     4981 atgcagtaac ggcaaccggt gcacacatct ttgtcgtaat ggacaatgcc ggttttcgga
     5041 tcttttttca gcgcagagac cgggcacaca gagacacagt tcggatcgac gcagtgcata
     5101 cactgtttct taatgtacgc gtagccgttc tcctcctggt ctttgttgac ccctgtgccg
     5161 ctggtccaca cctgaatgat gttattggta tacggcgaca gtttgtcgtt gttcgaccag
     5221 gtctgttccc cttgcgggtt acgttcaggg aagttgatat cctgacactt ggtgacgcaa
     5281 gcctggcagc ctacgcacaa ggtcgagtcg tacaacatcc ccagcgatcc cggaattggc
     5341 gggcggtttt cagcagccgc atgactgaca gacggcagcg cgcccgtcag caatgccccg
     5401 caggaggctg ctttaataaa attacgtctg ttcacggtta ttctccccgt gagtcagcgt
     5461 tatctttctt ttgctgacga cccagttcac gcaccgccat cacgctgaca ccggcaacca
     5521 gcccaaccac accgccgagc aaaccaatag cgcctgcaga gacgttgccg ccctctttag
     5581 cgttaacatc cggtttctgt gaacgcggag tctgattttc gacgttggca agctgatgga
     5641 tgcctttatg gaagccgata ccttcttcgt tacagccata gcatgggtga ccaatcgcta
     5701 ccggccacac gccgccaaca tcgcagaatt gcagcgttga gcagttgccg taagtttctg
     5761 gccctttaca gccgaggtgg tacaggcacc agccttcacg gtggccttca tcaccgaact
     5821 ctttggcaaa acgaccagca tcgaagtgcg ggcgacgttc gcagtgttcg tgaatcagac
     5881 ggccataggc gaaggtcgga cgatttttgt catccagttt cggcggtttg ccgtaagtga
     5941 tgatgtgcgc aacggtcgcg aggaagttgt gcgggttcgg cgggcagccc ggaatattga
     6001 taacggtttt gcctggcaga acttcttgca ggctgactgc gccagttggg ttaactccag
     6061 ctgcggcaac accgccccac gcagagcagg aaccgatagc gataatcgct gctgcgcctt
     6121 ctgccgcttt gcggatgtga tccacaatcg gctcaccagc aaccatgcaa taaataccgt
     6181 tatcttttaa tgggatggaa ccatccacaa ccaacacata ctgccctttg tacttctcaa
     6241 gtgcgttatg tttgttctct tcgacctgat gaccgaaggc ggcggaaagc acttcgtgat
     6301 actccagaga gatagtctcc agcacgaggt tttctaccgt tggatgcgtt gcacgaagca
     6361 gagattccgt acaaccggtg cactcctgcg cgccaatcca gataactggc ggacgctgcg
     6421 ggttagtaac cgattcggcc atctctgcag cggctttgct acttaacccc atggtggcgg
     6481 ctaatgctgc acaaagcttc atgaaatcac gacggttaat gccgtgagaa tggatgaggg
     6541 tgttatctcc agtcatttat agttattccg ttgcgaagac ctggcatata ttttgcctca
     6601 atcgcaaaat caataatgcg atcgatgcgc catttaccac acatttatta tggttatcga
     6661 tatcatgata ctgcggcggg gcgctaaaac gaagggaaga agcattagtg taattaatta
     6721 aagcaagata atacgtatgt ttgatcaatt ttcgaggcga taaccggcca taaaaaagcg
     6781 caacgtagaa caggaattat tctcacgaaa gaaatccatt accatctctt tatccagtcc
     6841 atagcggcgc gtcagaatac ccgcttccca ggcgctaagc tggcgatctc cggtcttttc
     6901 gtacatccgg tgtgagtagc ctaatacaaa accgcgttta taatcggcac agaattttgt
     6961 cacattaacc gcactgtcgg catgcgttgc ttttaatccc gccattaagc ctttaccaaa
     7021 atggttattc atcgttcacc tcattcgcta tatatcgcca tatatagcga tattttaggc
     7081 aaagaacgac ttttgcgcac gctatttttt catgcttgtt taagacgtaa tccagtaaga
     7141 gataaaccta tcaggagaga tatttatcga accatttcag tgtcctttgc caggcaagat
     7201 cggcggcaga tttgtcataa cggggcgtgg aatcattatg gaatccgtga ttaacccccg
     7261 gatagatata cgcctcataa accttattat tggctttcaa cgccgcctcg taagcaggcc
     7321 agccctcgtt gattcgggtg tccagttccg cgaagtggag tagtaaaggc gcttcaatct
     7381 tcgccacatc ggcagtgggt gcctgacgac cataaaacgg caccgcgcag gccagttccg
     7441 gatacgcgac agccgccgcg ttcgatacgc caccgccata gcaaaatccg gtaataccca
     7501 ctttgcctgc cgcttgcgga tagcgttgca taaactcaat tgcggcaaag aaatcattca
     7561 tcagtttggt tggatcaacc tgctgttgca gctcacgacc tttatcatca tttcccggat
     7621 aacctccaac ggaacttaag ccgtcaggtg ccagggcgat ataccccgcc ttcgccactc
     7681 gccgtgccac atcttcgata tacggattca gtccacgatt ctcatgcacc actaccacgg
     7741 ctggcgtttt gccgctcatt tttgcgggtt tcaccagata accccgtacc tcgccgtgac
     7801 catttggcga aggatacgtg atgtactcag caacaatctc cgggtcggta aactctacct
     7861 gagtcgccag cgcataattt ggcttgagca aatcaaacaa cgctaatgcc gtcatcccgc
     7921 ccaccgcata cttcgccgca aggttgagga actcacgttt cgagattttc ccgtgagcgt
     7981 aatagtcgta gtaatccaac aactcttgtg ggaaatcttt ggcggttaaa cgcggcatcg
     8041 ttgcactcct caactggtgt ttttttaagc aaagcataag cacgtgtttt tgcccagttt
     8101 ttcgtcactc tgtgagccag actacgggat acgcgctggc gaatcgctaa actagaaaca
     8161 ttgtttcgaa attgaacggt ggaaaggaga ggtcatggtc tggttagcga atcccgaacg
     8221 ttacgggcag atgcaatacc gctattgcgg aaaaagcggt ttacgcctgc ccgcgttatc
     8281 gctcggttta tggcacaatt tcggtcacgt taacgcgctg gaatcacagc gtgcgatcct
     8341 gcgtaaagcg tttgatttgg gcattacgca ctttgattta gccaacaatt acgggccgcc
     8401 tccaggaagc gcagaagaga actttggtcg cctgctgcgg gaggattttg ccgcttatcg
     8461 cgatgaactg attatctcta ccaaggctgg ctacgatatg tggcccggcc cttacggctc
     8521 tggcggttca cgtaaatacc tgctcgccag cctcgaccaa agcctgaagc gtatggggct
     8581 tgagtatgtc gatatctttt actctcatcg cgtcgatgaa aatacgccga tggaagaaac
     8641 cgcctctgcg ctggctcatg cggtacaaag cggtaaggcg ctgtatgtcg ggatctcctc
     8701 ttactcgcca gagcggacgc aaaaaatggt cgagttgctg cacgagtgga aaattccgct
     8761 tttaattcat caaccttcgt acaatttact gaaccgctgg gtggataaaa gcggcctgct
     8821 ggataccctg caaaataacg gcgtgggctg tattgccttt actcctctgg ctcagggatt
     8881 gctgaccgga aaatatctca acggcattcc gcaagattca cggatgtatc gtgaagggaa
     8941 taaagttcgt ggtctgacac cgaaaatgct taccgaagcc aacctcaaca gcctgcgctt
     9001 attgaatgaa atggcacagc agcgtggaca atcaatggcg caaatggcgt taagctggtt
     9061 gttgaaagat gatcgcgtga cgtcggtatt gattggtgcc agccgcgcgg agcaactaga
     9121 ggagaacgtg caggcgctga ataatctgac atttagcacc gaggagctgg cgcagattga
     9181 tcagcatatc gccgatggcg agctgaatct gtggcaggcg tcgtccgata aatgacctgt
     9241 taataacggg cgcgcgaacc gcgcccgaat cagatcagtg attatgacga gtcagtcggt
     9301 caagatagcc catcacaaat gcagaaagca caaacgtcag atggataatg acgtaccaca
     9361 tcagtttgtt atccgggaca tttttcgcat ccataaagac gcgcagtagg tgaatggaag
     9421 aaattgccac aatcgacgct gctactttgt ttttcagcga cgttgcgtcc attttcccca
     9481 gccagttcag cttctctttg ttctcggaga tatccagctg tgagacgaaa ttctcataac
     9541 cggaaaacat caccatcacc agcaaaccgc caaccagcgt catatccacc agcgacagca
     9601 acacgaggat caaatctgat tccgccatcg agaagatatt cggcagtacg ttaataatct
     9661 cctggaagaa cttcagcgcc agggcaacta acgccagcga aaggccaaag tacacggggg
     9721 caagcagcca gcgagaagca tacattgcat tttcaagaaa acgttccata cattcctgtc
     9781 gtgtagttaa aacgggcaac agtatatcgc aattgagtaa acagcacgca acagcaaatc
     9841 aacatatacg ttaaaccttt gtcgcataat tctgaggttt agggatcttc ctggttccct
     9901 tatcaacgca tcgagagcgt ctacccttaa aggacgttga acctgaaggg agaaaaacga
     9961 tgtctcattt aaaagacccg accacgcagt attacactgg tgaatatccc aaacagaaac
    10021 aaccgacgcc aggcatccag gcgaagatga caccggtacc ggattgcggc gagaaaacct
    10081 atgttggtag cggtcgcctg aaagatcgta aagcactggt gacagggggc gattccggaa
    10141 taggtcgcgc tgccgccatc gcttacgcgc gtgaaggggc tgacgtggcg atcagttatc
    10201 ttcccgtgga agaagaagac gctcaggatg tgaaaaagat cattgaagaa tgcggacgca
    10261 aagccgttct gctgccaggc gatttaagcg atgagaaatt cgcccgttcg ctggttcacg
    10321 aagcgcacaa ggcgttaggc gggctggata ttatggcgct ggtcgccggg aaacaggttg
    10381 ccattccgga tattgcagac ctcaccagcg aacagtttca aaagaccttt gccattaacg
    10441 ttttcgcgct gttctggcta acccaggaag cgatccccct gctaccgaaa ggtgcaagta
    10501 tcatcaccac ttcgtcaatc caggcatacc agccaagtcc gcatttactg gactatgcgg
    10561 ctacgaaggc ggcgattctg aactacagcc gtggcctggc aaaacaggtc gcggagaaag
    10621 gtattcgggt gaatattgtc gcgccaggcc ctatctggac ggcactgcaa atttccggcg
    10681 gacaaacgca ggataagatc ccgcagtttg gtcagaaaac gccgatgaag cgtgcggggc
    10741 aaccggcgga actggcccct gtatatgttt atctggcaag tcaggagtcg agctacgtca
    10801 ccgcagaagt gcacggtgtg tgcggcggcg agcatttagg ttaaaaaaat gcccggttgt
    10861 gaaaagcaac cgggcatcat tgtgaattca ctcttacctg atgtggcgaa aacgccttat
    10921 ccgcctacaa acgcatgaag attcaacatg ttgcaataga ttttgtaggc ctgataagcg
    10981 tagcgcatca ggcattgagc acctaatgcc ggatgcggcg tgaacgcctt atccggccta
    11041 caaacgcacg aagattcaac atgttgcaat agatattgta ggcctgatga gcgtagcgca
    11101 tcaggcaatt ctactttact tcgctttggc ggtttcttcg ccgaccagac ctatcttcag
    11161 gtaacccgcc tgatgcagcg tatccattac cttcatcaac gtctcgtaat cgacggtttt
    11221 atccgctcgg aagaagatgg tggtgtcttt cttgccttcg gttaacgcat tcaacgccgt
    11281 aatcattgtt tcatcggtga ccggatcgtt accgataaac atcgagttgt ctgccttcac
    11341 cgacagataa accggttttt ccgggcgcgg ctgcggcgtg ctggtagaag caggcaagtt
    11401 caccttcaca tctaccgtcg ctaacggtgc cgccaccata aagataatca gcagaaccaa
    11461 catcacgtcg ataaacggcg tcacgttgat atcatgcatt tcgccgttat cgtcgaggtt
    11521 ttcgttaaga tgcattgcca tcggatatta tcctgcgcgt aatttttgtg cgacacgaac
    11581 cggatgcgca gcggcgctgg cttccagatc caggtcacgg ctttgcagca acaatacctg
    11641 cgctgcaaca tcacccagca tcgctttaaa gccgccaatc tggcgtgcaa atacgttata
    11701 gataacgacc gcaggaatcg ctgccacgag gccgattgcc gttgctaaca gagcttctgc
    11761 gatacccggc gcaacgactg ccaggttagt ggtctgcgtt tgcgcgatac caataaagct
    11821 gttcatgatg ccccagaccg taccaaacag accaacgaac ggagaaatcg cgccgatggt
    11881 tgccagatag ccgttaccgc ggcccatttg acgacccact gcggcgaccc gacgctccag
    11941 acggaagctg gtacgttctt taataccttc gttatcgtcg ctgccttctg acagttccag
    12001 ctcattctgc gcttcattga gcaaatgcag gcttaagctt ttgctaccaa aatcagcggc
    12061 gatatcgttg gcctggttta aggaacgcgc ttcagccagc agttgctgct cgcgcttaag
    12121 gcgacgcttc tgattgaaga actctacgct cttactgaag aagattgccc aagtgactac
    12181 ggaggccaaa ataagcccaa tcatcacgca cttaacgaca atatcggcgt gctgatacat
    12241 accccagacg gaaaggtccg tctgcattaa attattaccc actgtgtatc tccaggacgc
    12301 aagtcacaaa atctgcgcat aataatatca aaatgacgtc gaattgatag tcgttctcat
    12361 tactatttgc atactgccgt acctttgctt tcttttcctt gcgtttacgc agtaaaaaag
    12421 tcaccagcac gccatttgcg aaaattttct gctttatgcc aattcttcag gatgcgcccg
    12481 cgaatattca tgctagttta gacatccaga cgtataaaaa caggaatccc gacatggcgg
    12541 acaaaaagct tgatactcaa ctggtgaatg caggacgcag caaaaaatac actctcggcg
    12601 cggtaaatag cgtgattcag cgcgcttctt cgctggtctt tgacagtgtg gaagccaaaa
    12661 agcacgcgac gcgcaatcgc gccaatggcg agttgttcta tggacggcgc ggaacgttaa
    12721 cccatttctc cttacaacaa gcgatgtgtg aactggaagg tggcgcaggc tgcgcgctat
    12781 ttccctgcgg ggcggcagcg gttgctaatt ccattcttgc ttttgtcgaa cagggcgatc
    12841 atgtgttgat gaccaacacc gcctatgaac cgagtcagga tttctgtagc aaaatcctca
    12901 gcaaactggg cgtaacgaca tcgtggtttg atccgctgat tggtgccgat atcgttaagc
    12961 atctgcagcc aaacactaaa atcgtgtttc tggaatcgcc aggctccatc accatggaag
    13021 tccacgacgt tccggcgatt gttgccgccg tacgcagtgt ggtgccggat gccatcatta
    13081 tgatcgacaa cacctgggca gccggtgtgc tgtttaaggc gctggatttt ggcatcgatg
    13141 tttctattca agccgccacc aaatatctgg ttgggcattc agatgcgatg attggcactg
    13201 ccgtgtgcaa tgcccgttgc tgggagcagc tacgggaaaa cgcctatctg atgggccaga
    13261 tggtcgatgc cgataccgcc tatataacca gccgtggcct gcgcacatta ggtgtgcgtt
    13321 tgcgtcaaca tcatgaaagc agtctgaaag tggctgaatg gctggcagaa catccgcaag
    13381 ttgcgcgagt taaccaccct gctctgcctg gcagtaaagg ccacgaattc tggaaacgag
    13441 actttacagg cagcagcggg ctattttcct ttgtgcttaa gaaaaaactc aataatgaag
    13501 agctggcgaa ctatctggat aacttcagtt tattcagcat ggcctactcg tggggcgggt
    13561 atgaatcgtt gatcctggcg aatcaaccag aacatatcgc cgccattcgc ccccaaggcg
    13621 agatcgattt tagcgggacc ttgattcgcc tgcatattgg tctggaagat gtcgacgatc
    13681 tgattgccga tctggacgcc ggttttgcgc gaattgtata acattgccac ttttggacaa
    13741 ttttgcagac atttaattgt gaaaagtctt aaattgttgc gtccgggatc aaggcgtccc
    13801 ggacgaatca ggagtacaat aggcagataa aggcttaaac gctgttccac aggaaagtcc
    13861 atggctgtta ttcaagatat catcgctgcg ctctggcaac acgactttgc cgcgctggcg
    13921 gatcctcata ttgttagcgt tgtttacttt gtcatgtttg ccacgctgtt tttagaaaac
    13981 ggcctgctgc ccgcctcatt tttgccaggc gacagcttgt tgatactggc aggcgcattg
    14041 attgcccaag gggttatgga ttttctgcct acgattgcga ttctgaccgc cgcagcaagt
    14101 ctgggctgct ggctaagtta tattcagggg cgctggttag ggaataccaa aacggtgaaa
    14161 ggctggctgg cacagcttcc tgctaaatat caccagcgcg ccacctgcat gtttgaccgc
    14221 cacggtctgc tggcgctgct ggctggacgt tttcttgcat ttgtccgtac gctgctgcca
    14281 accatggcgg gaatttccgg tctgccaaac cgccgcttcc agtttttcaa ctggttaagt
    14341 ggattgctgt gggtcagcgt ggtaaccagt tttggctatg ccttaagtat gattccgttc
    14401 gttaaacgcc atgaagatca ggtaatgacg ttcctgatga tcctgccaat tgccttgtta
    14461 accgctggct tgttaggcac gctgtttgtg gtgattaaaa aaaaatactg taacgcctga
    14521 cgattttccc cgttcccggc tgctgtaccg ggaacgtatt taattcccct gcatcgcccg
    14581 cattcttgcc gcatcttccc ccggcgtcac accgaagtaa cgtttaaact cacggctaaa
    14641 ttgcgatgcg ctttcatagc cgacgcgcat cgctgctgcg ctggccttca tgccgtcatg
    14701 gatgatcatc atccgcgcct tatgcagacg gtaattcttc aaatactgca acggcgaggt
    14761 gctggtgaca gacttaaaat tatggtggaa cgccgatacg ctcatgttgg cttctgccgc
    14821 cagttgctcg acgctcaggt tttcggtgta tttattctca atccgtttca gcacgcggct
    14881 aatcagactg aagtgagtct ggcgactgac cagcgccagt aacgcgccgc cgcaaggtcc
    14941 ggtcagcacg tagtacagaa tttcgcggat gatctgtttg ccgagaatac gcgcatccag
    15001 tggtcgctcc atcacgtcga gtaaccgctc cgccgcgcat aaaatctctt ctgataacgt
    15061 ggcggagtta atcccgctgg ctgccatcga cggctggaaa tgctcatctt cgccaatgtc
    15121 catcaacagt tcctgtaact gcaaaatatc gacattgaga cgcaaccctg ccagcggcac
    15181 ctctgacgtg gcataggttt cgcactcaaa cggcaacggc accgtcagca gcaggtattc
    15241 attggcatca taacgaaaca cgcgttcatt gatataaccg attttatgcc cggaaaagag
    15301 aattatgatg ccaggctcgt acatcaccgg tgtacgtgcg aaaggcgtct cgccatacaa
    15361 caaacgtaca tcgggcaaca gttctgacaa actattttct ttaattttca gtttattaac
    15421 tttatccgcc agcaagcggc aaatctcttc acgtttcata tcgcgtaatt tcttaggaat
    15481 aatgcggcaa tttgattgtg cgcaattttg tagcatttct ccagcactct ggaggaatag
    15541 gcaagacatt ggcagaaatg agcattgaga gccagggcgc tggcgatcac aatgaaaaac
    15601 atcaggcaga tcgttctctg ccctcatatt ggcccagcaa agggagcaag taatgaacaa
    15661 ctttaatctg cacaccccaa cccgcattct gtttggtaaa ggcgcaatcg ctggtttacg
    15721 cgaacaaatt cctcacgatg ctcgcgtatt gattacctac ggtggcggca gcgtgaaaaa
    15781 aaccggcgtt ctcgatcaag ttctggatgc cctgaaaggc atggacgtgc tggaatttgg
    15841 cggtattgag ccaaacccgg cttatgaaac gctgatgaac gccgtgaaac tggttcgcga
    15901 acagaaagtg actttcctgc tggcggttgg cggcggttct gtactggacg gcaccaaatt
    15961 tatcgccgca gcggctaact atccggaaaa tatcgatccg tggcacattc tgcaaacggg
    16021 cggtaaagag attaaaagcg ccatcccgat gggctgtgtg ctgacgctgc cagcaaccgg
    16081 ttcagaatcc aacgcaggcg cggtgatctc ccgtaaaacc acaggcgaca agcaggcgtt
    16141 ccattctgcc catgttcagc cggtatttgc cgtgctcgat ccggtttata cctacaccct
    16201 gccgccgcgt caggtggcta acggcgtagt ggacgccttt gtacacaccg tggaacagta
    16261 tgttaccaaa ccggttgatg ccaaaattca ggaccgtttc gcagaaggca ttttgctgac
    16321 gctgatcgaa gatggtccga aagccctgaa agagccagaa aactacgatg tgcgcgccaa
    16381 cgtcatgtgg gcggcgacgc aggcgctgaa cggtttgatt ggcgctggcg taccgcagga
    16441 ctgggcaacg catatgctgg gccacgaact gactgcgatg cacggtctgg atcacgcgca
    16501 aacactggct atcgtcctgc ctgcactgtg gaatgaaaaa cgcgagacca agcgcgctaa
    16561 gctgctgcaa tatgctgaac gcgtctggaa catcactgaa ggttccgatg atgagcgtat
    16621 tgacaccgcg attgccgcaa cccgcaattt ctttgagcaa ttaggcgtgc cgacccacct
    16681 ctccgactac ggtctggacg gcagctccat cccggctttg ctgaaaaaac tggaagagca
    16741 cggcatgacc caactgggcg aaaatcatga cattacgttg gatgtcagcc gccgtatata
    16801 cgaagccgcc cgctaagctt tttacgcctc aaactttcgt tttcgggcat ttcgtccaga
    16861 cttaagttca caacacctca ccggagcctg ctccggtgag ttcatataaa ggaggaacgt
    16921 atggctaatc caaccgttat taagctacag gatggcaatg tcatgcccca gctgggactg
    16981 ggcgtctggc aagcaagtaa tgaggaagta atcaccgcca ttcaaaaagc gttagaagtg
    17041 ggttatcgct cgattgatac cgccgcggcc tacaagaacg aagaaggtgt cggcaaagcc
    17101 ctgaaaaatg cctcagtcaa cagagaagaa ctgttcatca ccactaagct gtggaacgac
    17161 gaccacaagc gcccccgcga agccctgctc gacagcctga aaaaactcca gcttgattat
    17221 atcgacctct acttaatgca ctggcccgtt cccgctatcg accattatgt cgaagcatgg
    17281 aaaggcatga tcgaattgca aaaagaggga ttaatcaaaa gcatcggcgt gtgcaacttc
    17341 cagatccatc acctgcaacg cctgattgat gaaactggcg tgacgcctgt gataaaccag
    17401 atcgaacttc atccgctgat gcaacaacgc cagctacacg cctggaacgc gacacacaaa
    17461 atccagaccg aatcctggag cccattagcg caaggaggga aaggcgtttt cgatcagaaa
    17521 gtcattcgcg atctggcaga taaatacggc aaaaccccgg cgcagattgt tatccgctgg
    17581 catctggata gcggcctggt ggtgatcccg aaatcggtca caccttcacg tattgccgaa
    17641 aactttgatg tctgggattt ccgtctcgac aaagacgaac tcggcgaaat tgcaaaactc
    17701 gatcagggca agcgtctcgg tcccgatcct gaccagttcg gcggctaaca tgcaaattct
    17761 cccggtggcg gtaatgttcc gctaccggac ttttcagaaa tcatttattc ccctcgcgtc
    17821 ccgcccgttg ttactcttcc ttgttcagga atgccaaata taaggacatc atcatgcaga
    17881 gccggaagct cttaaaagaa caactcatct atatccggga taaacgcaac ggagaggtga
    17941 aaaacagatg aaaataatac ttctgttttt agcagccctg gcaagtttta ccgtacacgc
    18001 acagcccccc tcactgaccg tagaacaaac agtccggcat atttatcaga actataaatc
    18061 agatgccact gccccttatt ttggtgaaac cggagagcgg gcgataactt ctgcgcgtat
    18121 tcaacaggcg cttaccctga acgacaatct tacgctgccg ggcaatattg gctggctgga
    18181 ttatgatccg gtttgtgatt gtcaggattt tggcgatctg gtgctagaaa gcgttgcgat
    18241 aacccaaact gacgccgatc atgccgatgc cgttgtgcgc tttcgtatct ttaaagatga
    18301 taaagaaaag accacgcaga cactgaaaat ggtggcggaa aatggtcgtt gggtcattga
    18361 cgatattgtc agcaatcatg gcagcgtctt acaagcagtt aatagcgaga atgaaaaaac
    18421 gctggccgct ttagcttcgt tgcaaaaaga acagccggaa gcctttgttg ccgaactctt
    18481 tgaacatatt gctgattata gctggccgtg gacgtgggtg gtttccgact cttaccgcca
    18541 ggcggttaat gccttctata aaaccacctt caagacggcc aataatcccg atgaagatat
    18601 gcaaatagaa cggcaattta tttacgacaa tccgatctgt tttggcgaag agtcgctatt
    18661 ttcacgcgtt gatgaaattc gagtcctgga gaaaaccgcc gattccgccc gtattcatgt
    18721 tcgttttacg ctgaccaatg gcaacaacga agagcaagaa ctggttttac agcggcgcga
    18781 aggcaagtgg gaaatcgctg attttatccg cccgaacagc ggcagcctac ttaagcagat
    18841 tgaggcaaaa accgccgcca gattaaagca atgagctgaa ttaaataaca attagccgga
    18901 acaataaata aaagggaaca ctatatgaaa acgattttca ccgtgggagc tgttgttctg
    18961 gcaacctgct tgctcagtgg ctgcgtcaat gagcaaaagg tcaatcagct ggcgagcaat
    19021 gtgcaaacat taaatgccaa aatcgcccgg cttgagcagg atatgaaagc actacgccca
    19081 caaatctatg ctgccaaatc cgaagctaac agagccaata cgcgtcttga tgctcaggac
    19141 tattttgatt gcctgcgctg cttgcgtatg tacgcagaat gataaaaaaa tccccggcag
    19201 catgtcagtt gccggggatt ttttttaacg tccaaccgcc gctttagggc gtttcttcgc
    19261 accagcattc accggacgag attgcgtaga cgacgctttt tttgccgtag caggcgtctg
    19321 acgctgggtc gccatcggcg tatgtttcgt caacgccgga cgggtattgc ggttctggcg
    19381 acgagcttca cgcatctctt caatggttgg cgcaggcact aagcaatcgc gacggctgcc
    19441 aatcagatgc tttttgccca tcgcttccag cgcctggcgg attaacggcc agtttgccgg
    19501 atcgtggtaa cgcaacaacg ctttatgcaa acgacgctgt ttgtcgccct tcggtacgaa
    19561 gacgtcttcg ctcttataac caatcttcgc cagcgggttt ttcccggtgt aatacatggt
    19621 ggttgagttc gccagcggcg atgggtagaa gttctgtacc tggtcgagac ggaaacgatg
    19681 ctttttcagc cacagcgcca gattcaccat atcttcatca cgcgtaccgg ggtgcgcgga
    19741 gatgaaatag gggatcagat actgctcttt acctgcctgt tttgagtaag tatcgaacag
    19801 ctctttaaag cggtcatagc tgcccatgcc cggcttcatc atcttcgata acggcccttc
    19861 ttcggtatgt tccggggcaa tcttcagata accgccgacg tgatgggtcg ccaactcttt
    19921 gatatagcgc ggatcttcca cggcgatgtc ataacgtacg cctgaggcga tgaggatttt
    19981 tttaatgcct ttcagatcac gcgcacggcg atagaggttg atcgtcggtt cgtggttagt
    20041 gtccatgtgc ggacaaatat ccggataaac gcacgacaaa cggcgacaag tttgttcagc
    20101 gcgtggcgat ttgcagcgca acatatacat gttggcagtt ggcccaccga gatcggaaat
    20161 tacgcccgta aaaccaggaa cggtgtcgcg gatcgcttcg atctcattaa tgatcgaatc
    20221 ttccgaacgg ctctgaataa tgcgcccttc gtgctcggtg atagaacaga aagagcagcc
    20281 gccaaagcag ccgcgcataa tgttgaccga gaaacggatc atttcgtaag ccggaatacg
    20341 ggcattgcca taggccggat gcggcacgcg cttgtacggc agcgcaaaaa cgctgtccat
    20401 ctcttcggta gaaagcggga tagcaggcgg gttgatccac acatagcggt caccgtgttt
    20461 ttgcatcaat gcgcgggcgc agcctgggtt ggtttcgtgg tgcagaatgc gcgaagcatg
    20521 agcgtacagg actttatcgc ccttcacttt ctcgaaagaa ggcagcaata cgtaggtttt
    20581 ttcccacggt ttcgggcgcg gcggctgcac ggttacagct ttcgcttcct gctttttcgg
    20641 cgcaacaggt ttgttatccg cgcacggcag atcttcgcca tacggatgcg ggattgggtc
    20701 gattttccct ggggtatcaa gacgagtaga atccacaccg ctccagccag gcagcgcctc
    20761 ttttacgata attgcggtat tacgcacatc gcggatttcg ctaattggct cgcccatcgc
    20821 cagacgatgc gctacttcca ccagcggacg ttcaccgtta ccaaacatca gcatgtcggc
    20881 tttcgaatcc accagcacgg aacggcgcac ggtatcggac cagtaatcat aatgcgcggt
    20941 acggcgcaga ctagcctcaa taccgccaag gatcaccggc acatctttcc acgcctcttt
    21001 acaacgctgg gtataaacca gtgtggcgcg atccggtcgc ttacccgcga cgttatccgg
    21061 tgtgtaggca tcgtcatgac gtaaacggcg atcggcggta tagcggttga tcatcgaatc
    21121 catgttgcca gcagtaacac caaaaaacag attcggttta cccagacgca taaagtcgtc
    21181 tttgctgctc cagtccggct gggcgatgat cccgacgcga aagccctgcg cttccagcat
    21241 acgaccgcaa atcgccatcc cgaagcttgg gtgatcgaca tacgcgtcgc cagtaaccaa
    21301 aatgatgtcg cagctatccc agccaagttg atccatctct tcacgagaca tcggcaaaaa
    21361 cggtgccggt ccaaaacagg cggcccagta ctgcggccag gagaacaggt cgcgatccgg
    21421 ttggatcagg gagatagagc tcattttgct tccagaaatg ataaaaaaat aatcaaaggc
    21481 cggggattat aagccggaac gaaagagaaa tcgaaaggta ttccatactc gccctcctcg
    21541 ggcgagtatg aagattacgg taccggattg accaacagtt gcccaatcga gccacggtcc
    21601 gccatttcca gcgtctgact gttgaagtag aacgggaagt gcgcccagga aggctgaccg
    21661 aaataaacaa gcagctccac ttgtccgtca acccaaacgg tatctttcca gcctctgtct
    21721 tccgggaacg gcatcgcgcc attcacgtta cggatctgga acatcacgcc ttcaatatgg
    21781 aacgcttgcg gctcgtccgc gcgtaccgtc cagcgttccc acgttccttg ctgcgcggtg
    21841 acatcaatac ggttgacgtc ccacagctgt ccattaatac ccgggtcatc acccagacta
    21901 atatcgcgac tgcgaatcgg cgaaccggcc atgatttcag ttggcagcaa gcgcatcgga
    21961 agactgtctg tgaccagcgg cagaaggccg gttgggcgca gcgttagcac cagggtagaa
    22021 accaaaatgc tggatggctc aaagaagcca cgaatacgat caacaatgct cgccgcttcg
    22081 ccacaggtga tcgacacttc atcaccgttg ctcatatcca ccagaatctc gcggcgctcg
    22141 cccggtgcca gcgaaagttg cttcaccgat acaggagcag gcaggaatcc ctgatcgcca
    22201 gaaatcacat gtaacgggcg accatcgctc atctgtagtt gatagcgacg agagttcgac
    22261 gcgttcagca gtcgcaagcg cacccagcca cgcgagactt caacgtacgg gctttgtaca
    22321 ccgttaacca gcagcgtatc accaacaaag ccgccgcttc ccggttcgtt gtattctggc
    22381 gtaccaaagt tatccagccg tttatcctgg ataatgaccg gaaaatcatc cacaccataa
    22441 tggttgggga taggcagcga cttgctgact tcatcttcca ccagccacat tccggcaagg
    22501 ccgttataga cctgctgagc cgtgcggttg ggagtattgg cgtgatacca cagagtagct
    22561 gcgttctggc gaatgggcag tacgggtgcc cagtcagcgt ttggcgacat catccgtgcc
    22621 ggaccgccca tcagcggacc tggtacctgt agcccggcca ccgtcattga gacattttct
    22681 gtcaggcggt tgctgtaaat aagcttaaca tcgtcgccct tccagacgcg gatagtcggc
    22741 cccaggtaac gaccattgat tccccagacc gacgcgcgtg tccctggcgt aaatgaccag
    22801 tgcgcacgtt gtacagtcat aaacagcggt tgcccacggc gagattcaag tagcggcgga
    22861 acgggtagcg gttgctgttg cccggctgcg ctggccttca ggggaacagc gcctgcacaa
    22921 agtgcaatcc ccgatgcctg aatgaactga cgccgactga gtgacatatt tgctccatgt
    22981 aaaactggct aaagtaatac cgtgcaggaa agtgttcccc gcaaaaatac agattgccga
    23041 cttaaacttt tccggcggct tcgcgttctg cgacttcttt atcgagctcg gcgatttttt
    23101 gttccattat cgaacgacaa tgggcagcca gctcacgaac ctgatctttg ccatactgac
    23161 tgacgtcaat tggcggcagc atttcgacaa tcaccagacc gttgtgcagt cgattaagat
    23221 taatcttatt cgaagttgta gagacgcaca cgggaataat cgggacgccc gccgcaattg
    23281 ccgcgtgaaa tgctccagtc ttgaacggta gcaggccgcg accacggctg cgggttcctt
    23341 ccgggaacat ccagatggaa atacggcgtt ttttgaaatg attcacgact tccgcaatgg
    23401 tgccgtgagc tttagtgcga ttgtttctgt cgatcaataa gttgccggtt aaccagtaca
    23461 actgcccgaa gaaggggatc cacagcaagc tctttttacc taccgtcacc gtcggcggtt
    23521 gcacaatgtt cgatgctgtc accatgtcat agttgttctg gtggttagcg atatagatag
    23581 cattgccgta gctttcagca tctgcaggtt tgcggcactc aactttcagg ccgaacagcg
    23641 gcgcaagacg gccaaacata tgcccaaagg tggccacatg tttcgggtta cgcgggctga
    23701 aaaggcagta aatggagccg aatacacaga ctaagatgct gtaaatcacg gtaataataa
    23761 gacgaaagat atatagcata acaccctctg aaggtctgag agactggcat tgtacgtcac
    23821 ctgaaatcag gttagggcat tgttaaaagc cgctctctgg aaaagcgtat tgttaatcgc
    23881 aacgcatgaa taaacaacgg tttcaaggga atttttatcg tttccggggg ttgaatacgc
    23941 tgccggatga cgacttaacg tttcatccgg cgttccttgc aagcgggagg aaacaggccc
    24001 tccccggcat attactcttc gctatcaccg ctgctggcac ggcgaggaga gtcgatctca
    24061 acacgatcga tacgctgcaa accgcgcatc aacgtaccgc ggcgtccacg ttcgccagtg
    24121 actttctgta actcttccgg gcgcagttta attttgcgtt tcccaacatg aatggtcagc
    24181 gtgctttgcg gcggcagaac gtacaattgc gccagaccat cttctccacg cgcggcttct
    24241 gccgatggaa tgttgataat cttgttgcct ttgcccttcg acagctgcgg cagatcgctt
    24301 accgggaaca tcaacatacg gcctgcctga gtgattgcca gcagcatatc ggaagcatct
    24361 tcaatcacca ccggcggcat aacatgggca ttttccggta aggtgatcaa agccttacct
    24421 gcacggttac gcgccaccag atcgttaaag gtgcagacga aaccgtaacc cgcatcggaa
    24481 gccatcagca gtttctgatc gtcgctttcc atcagcatat ggtcaacggt cgccccaggc
    24541 ggcaacgtta atttgccggt gagtggttcg ccctgaccac gcgccgacgg cagcgtaatc
    24601 gggtcgatgg catagctacg accggtggaa tcaacaaaca ctaccggttg gttgctctta
    24661 cctttcaccg ccgctttgaa gctatcaccc gctttataat tcaggcccgg cgcgtcgata
    24721 tcatggcctt tagcgctgcg tacccagccc atctgcgaca gcacaatggt gacaggttca
    24781 gacggcagca tgtcgtgctc gctcatcgct ttcgcttctt cgcgttcctg caacggcgaa
    24841 cgacgatcgt caccgtaggc ttgcgcgtct gcctgcagtt ctttcttcag caggttattc
    24901 attttacgct cggaagccaa aatgccctgc aactggtcgc gctctttttc cagctcactc
    24961 tgctcaccgc gaatcttcat ctcttccagt ttggcaagat gacgcagttt cagttcgagg
    25021 atcgcttccg cctgggtttc cgtaaggcca aaccgcgaca tcagcgccgg tttcggttca
    25081 tcttcgttac gaatgatctc aatcacttcg tcgatgttga gaaacgccac cagcaaacct
    25141 tccaggatat gcaggcgctt gaggactttc tccagacgat agttcagtcg gcggcgcacg
    25201 gtatcgcgac ggaacaccag ccattcggag aggatttcca gcaggttttt caccgccgga
    25261 cgaccatcca gaccgatcat attgaggtta atgcgatagc tcttttccag atcggtggta
    25321 gcgaagaggt ggttcatcac ctgatccata tccacgcggt tggaacgcgg cacaatcacc
    25381 agacgggtcg ggttctcgtg gtcagattca tcgcgcagat cgtcaaccat cggcagcttt
    25441 ttgttgcgca tttgcgcagc aatttgctcc agtacgcgcg cacctgaaac ctgatgcggt
    25501 aatgcgctga taaccaccgc gccatcttct ttcttccaca ccgcgcgcat acgcactgaa
    25561 ccacgtccgt tctcgtagat tttacggatc tcggcgcgcg aagtgataat ttccgcttca
    25621 gtcggataat ccggcccctg cacgatatcc agcagctgct cgagcgtggt tttcggctgg
    25681 tcgattaatg cgattgccgc ctgagctact tcacgcaggt tatgcggtgg aatatcggtc
    25741 gccatgccga cggcaatacc ggtggtgccg ttaagcaaaa tgtttggcag acgagcaggc
    25801 agcattttcg gctcctgcaa agtgccgtcg aagtttggca cccagtcagc cgtcccctgc
    25861 cccagctcgc tcaatagcag ctcggaatat ttcgacaacc gggattcggt gtaacgcatt
    25921 gccgcgaacg atttcggatc gtccggcgcg ccccagttcc cctgaccatc aaccagcgga
    25981 taacggtaag agaacggctg cgccatcagg accatcgctt cataacaggc gctatcgccg
    26041 tgcggatggt atttacccag tacgtcaccg acggtacggg ccgatttttt aaatttggcg
    26101 ctggcattca ggcccagttc agacatcgca tacacaatgc ggcgctgaac aggtttaaga
    26161 ccatcgccaa taaacggcaa cgcacggtcc atgatcacgt acatggagta gtttaagtag
    26221 gcgttttccg taaattcatg tagcgcaagg cgctctgcca tatcgctcat taattctgat
    26281 tcctcaactt attcgccagc cttcaaaggg gcaatattgc cggagatact acattatctg
    26341 ccgcgtcgag tcacaggaga tcagcgtgaa tagcgccgcc ggacgtgctc cgacggcgat
    26401 aaaggcatca atgtgccttg atatacaact ctttgctgta gacttgatcg gcatagaggg
    26461 cagcacaagg ttgcgcccat gccacaccac tcggcatggg ggtccgctcc gggcgattcg
    26521 agaagttgag gttatgcgag tccagggaag cctaaagcat caaagtagtc gactgtaagt
    26581 atacggttca gcccagttta gctgtgtctc caccagtgcc ccgcgcctcc tgccataagt
    26641 gcggcttgct catggatacc cccgagacgg cttacggcgc accggataat aaatttcgcg
    26701 tatacatggt attccttacc ggagaaatta taagcccaac aacaatacgc ctgtggcatt
    26761 gttcgctcaa acttatcgcg agtgatttgg ctcacactcc ggtaaagatt ccccatcacg
    26821 ccaacggcac ataaacatcc gtttccagtc gctgctctgt caccccttcg gcaagattgg
    26881 tgtagtgaaa cataatcggt gctttacgca ttttctcgcc gcttgcaggc agccagtggc
    26941 gaataatgcc ccatatcgtg tgtgaaatat cgtccagctc gccaacgtgg cgggccacgg
    27001 cataacgtcc accggtaagt tcaccattgc tcacaccata acgattatcg ggaatcggtt
    27061 cgctaacgct gccgcagata tcaaagcgaa acgcttccgg tgcggtggtg gcagggtcgt
    27121 cccaggcgac gccaaaagtc tggctttggt taacaggaga aagccccgtc tctttacgcc
    27181 acatgataaa tttcgccaca ctgtagttga gcaactcagg gctacagcgg tgcggcaaca
    27241 tcgccacagg gattgatggg aaatcgataa tgtttacatc cagtgtcagg tttgtcattt
    27301 tgcctccctg cgaaattatt cccgggaacg cgtcccggga gcggtaacaa ttacggattt
    27361 actttgcgga tctgtttgac gtcaatttca acagaattcc agtctttatc gacttcaccc
    27421 tgaatctcaa ccgtatcttt cggcgtcacc gtcacaccgt tccagcgttt gtggtcgata
    27481 tcaacattga tagtaccgct ggcatcttta aacacgtaga gatcgtcaga gatgcgttca
    27541 acgatattgc cgcgcagggt tacccaggtg tcgtcacgca gggattttgc gctttctacg
    27601 gtcgttacgc tgccgttcgg cccctggaat cctccggcct gactttgcgt tgccgatggg
    27661 ccagaaaaac cgccctgctc tgctgccatc accggtgcgc tgcacagggc cattactgcg
    27721 attactgctg cgaatttttt catgtttatt actcccttta atgtctgttt ccgagcattt
    27781 aacaagatag tccttaacaa cttcttaagg gaaaaaaata aaatttagtg ctgtacagag
    27841 cgcgttacaa cacggtttac tggcagcaaa tacggttatc gcagggatga aaaaatgcga
    27901 attttactga tagaagatga catgctgatt ggcgacggca tcaaaacggg ccttagtaaa
    27961 atgggtttta gcgtcgactg gtttacacaa ggtcgtcagg gaaaagaggc gctttatagc
    28021 gcgccttatg atgcggtgat cctggattta accttaccag gcatggatgg tcgcgatatt
    28081 ttgcgcgaat ggcgagaaaa aggtcagcgt gagccggtac tgatcctgac cgcgcgcgat
    28141 gcgctggagg aacgtgtaga agggctgcgt ctgggagctg acgattatct gtgtaaacct
    28201 tttgcgttga tagaagtcgc cgccaggctg gaagctctga tgcgccgaac caacggccag
    28261 gccagcaacg agctgcgcca cggtaacgtc atgctcgacc ccggcaaacg tatcgccacg
    28321 ctggctggcg aacccttaac actgaaacca aaagaatttg ccctgctgga attactgatg
    28381 cgtaacgctg gtcgggtact gccgcgcaaa ctgattgaag agaaactgta tacctgggac
    28441 gaagaggtca ccagtaatgc cgttgaagtg catgtgcatc atctgcgacg caaactcggt
    28501 agtgatttta ttcgtaccgt gcatggtatt ggctacacat taggtgagaa atgaaattta
    28561 cccaacgtct tagtctgcgc gtcaggctga cgctaatctt tttaattctg gcctcggtga
    28621 cctggctgct ttccagcttt gtcgcctgga aacaaacaac ggataacgtc gatgaattgt
    28681 tcgacaccca actgatgctg tttgccaagc ggttaagtac gctcgatctc aacgaaatca
    28741 acgcggcgga tcgcatggca cagacgccaa ataaattaaa acacggtcat gttgatgacg
    28801 atgcgctgac ctttgccatc tttacccacg acggcagaat ggtccttaac gatggcgata
    28861 acggagaaga tattccttat agctatcaac gggaaggttt tgctgacggg caactggtgg
    28921 gcgataaaga tcaatggcgt tttgtctgga tgacctcacc tgatggcaaa tatcgcatcg
    28981 ttgttggtca ggaatgggaa taccgtgaag acatggcgct ggcgattgtt gccgggcaat
    29041 tgatcccgtg gctggtcgca ctgccgatta tgttaatcat catgatggta ctattgggtc
    29101 gtgaactcgc gccgctgaac aaactggcgc tggcgctgcg tatgcgtgac cctgactcgg
    29161 aaaaaccact aaacgcgact ggcgtaccca gcgaagtgcg tccgttggtt gagtcgctaa
    29221 atcaactgtt cgcccgcaca catgcgatga tggttcgtga acgacgcttt acctccgacg
    29281 cagctcacga actacgtagc ccgttaacgg cgctgaaagt gcaaaccgaa gttgcgcagc
    29341 tttctgacga tgatccgcaa gcgcggaaaa aagcgctgct tcaattacat tacgggatcg
    29401 atcgcgctac tcgtctggtt gatcaactgc tcactctatc gcggctggac tcactggata
    29461 accttcagga cgtcgcggag atcccgcttg aagatctcct gcaatcgtcg gtgatggata
    29521 tttaccacac ggcgcagcag gcgaaaattg acgtgcgact gacactcaat gcccacggca
    29581 tcaaacgcac cggacaaccg ctattgctaa gtttgttggt gcgaaatttg ctggataacg
    29641 ccgtgcgcta cagtccacag ggcagcgtga tagacgtcac gctgaatgct gataatttca
    29701 tcgtgaggga taacggcccc ggtgtgacgc cagaggctct ggcgcgaatt ggcgaacgct
    29761 tctatcgccc ccccggacaa accgctaccg gcagtggact tgggctatcg attgtccagc
    29821 gaatcgccaa attgcatggc atgaatgttg agtttgggaa tgcggaacaa ggtggatttg
    29881 aggcgaaggt aagctggtaa cttctctgtc tatgagagcc gttaaaacga ctctcataga
    29941 ttttactaat agcaaaatat aaaccgtccc caaaaaagcc accaaccaca aaccagacaa
    30001 ataccgagcg agaatacata tacccaaaac tcgaacattt cccgcataaa gagttttctt
    30061 aagataagaa taataagtgg cgtaagaaga aaaaatgctg caatcattgc ttgcaatata
    30121 cgatcttcgc ctgaaacgga aggtatactg caaatattgt ctcccgactc aagcatccag
    30181 tcatacttat tgaaagcaaa aaaagcatc
//
LOCUS       DAZKHF020000026        30194 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2201S31241
            SAMN43267800-rid25106413.denovo.026, whole genome shotgun sequence.
ACCESSION   DAZKHF020000026 DAZKHF020000000
VERSION     DAZKHF020000026.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267800
            Sequence Read Archive: SRR30307263
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 30194)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 30194)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 30194)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350581.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350581.2
            Genome Coverage       :: 151x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:21:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,652
            CDSs (total)                      :: 5,557
            Genes (coding)                    :: 5,320
            CDSs (with protein)               :: 5,320
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 237
            CDSs (without protein)            :: 237
            Pseudo Genes (ambiguous residues) :: 0 of 237
            Pseudo Genes (frameshifted)       :: 102 of 237
            Pseudo Genes (incomplete)         :: 130 of 237
            Pseudo Genes (internal stop)      :: 52 of 237
            Pseudo Genes (multiple problems)  :: 41 of 237
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..30194
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267800-rid25106413.denovo.026"
                     /serotype="O 26:H11"
                     /isolate="2201S31241"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-01"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            178..1093
                     /locus_tag="ACHOSC_RS04965"
                     /old_locus_tag="pgaptmp_000993"
                     /pseudo
     CDS             178..1093
                     /locus_tag="ACHOSC_RS04965"
                     /old_locus_tag="pgaptmp_000993"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005227760.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
     gene            complement(1287..2591)
                     /gene="ybtS"
                     /locus_tag="ACHOSC_RS04970"
                     /old_locus_tag="pgaptmp_000994"
     CDS             complement(1287..2591)
                     /gene="ybtS"
                     /locus_tag="ACHOSC_RS04970"
                     /old_locus_tag="pgaptmp_000994"
                     /EC_number="4.2.99.21"
                     /EC_number="5.4.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005227761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="yersiniabactin biosynthesis salicylate synthase
                     Irp9/YbtS"
                     /protein_id="HGU4546977.1"
                     /translation="MKISEFLHLALPEEQWLPTISGVLRQFAEEECYVYERQPCWYLG
                     KGCQARLHINADGTQATFIDDAGEQKWAVDSIADCARRFMAHPQVKGRRVYGQVGFNF
                     AAHARGIAFNAGEWPLLTLTVPREELIFEKGNVTVYADSADGCRRLCEWVKEAGTTTQ
                     NAPLAVDTALNGEAYKQQVARAVAEIRRGEYVKVIVSRAIPLPSRIDMPATLLYGRQA
                     NTPVRSFMFRQEGREALGFSPELVMSVMGNKVVTEPLAGTRDRMGNPEHNKAKEAELL
                     HDSKEVLEHILSVKEAIAELEAVCQPGSVVVEDLMSVRQRGSVQHLGSGVSGQLSENK
                     DAWDAFTVLFPSITASGIPKNAALNAIMQIEKTPRELYSGAILLLDDTRFDAALVLRS
                     VFQDSQRCWIQAGAGIIAQSTPERELTETREKLASIAPYLMV"
     gene            complement(2619..3899)
                     /gene="ybtX"
                     /locus_tag="ACHOSC_RS04975"
                     /old_locus_tag="pgaptmp_000995"
     CDS             complement(2619..3899)
                     /gene="ybtX"
                     /locus_tag="ACHOSC_RS04975"
                     /old_locus_tag="pgaptmp_000995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005227762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="yersiniabactin-associated zinc MFS transporter
                     YbtX"
                     /protein_id="HGU4546978.1"
                     /translation="MSDVQSNVKPLTLTTGRVIFAIAGVYVTQSLVSALSMQSLPALV
                     RAAGGSLALAGATTLFMLPWALKFIWAPWIERWRLPPGSQERRSRMLILRGQVALAAI
                     LTIAAAIGWFRREGGFPDTQIVALFVLFMVAGTVASTIDIASDGFCVDQLTRTGYGWG
                     NSVQVGGSYLGMMCGGGVFMMLSAASGWPVAMLMMAVLIMALSLPLWRITEPTRTATI
                     LHVPALGYALRRKQARLGLLLVLMLNSGMRFVLPLLAPLLLDHGLSMSALGALFSGGN
                     IAAGIAGTLAGGLLMKYTSPGRALLTAYGVQGIALLAVVMTLMMAPGHLLLQILQCLV
                     IVQSISLACALVCLYATLMSLSSPLQAGVDFTLFQCTDAAIAILAGVIGGVVAQHFGY
                     AACFLFAGAFTLLAAWVAYIRLHSARELMTSAID"
     gene            complement(3892..5694)
                     /gene="ybtQ"
                     /locus_tag="ACHOSC_RS04980"
                     /old_locus_tag="pgaptmp_000996"
     CDS             complement(3892..5694)
                     /gene="ybtQ"
                     /locus_tag="ACHOSC_RS04980"
                     /old_locus_tag="pgaptmp_000996"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005227763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="yersiniabactin ABC transporter
                     ATP-binding/permease protein YbtQ"
                     /protein_id="HGU4546979.1"
                     /translation="MKDNNPADNLAWRVIWRQLISSVGSQARMLRRSMLALLLAAFMQ
                     GIAFACLYPIIDALLRGDAPQLLNWAMAFSVAAIVTLVLRWYGLGFEYRGHLAQATHE
                     LRLRLGEQLRRVPLEKLQRGRAGEMNALLLGSVDENLNYVIAIANILLLTIVTPLTAS
                     LATLWIDWRLGLVMLLIFPLLVPFYYWRRPAMRRQMQTLGEAHQRLSGDIVEFAQGMM
                     VLRTCGSDADKSRALLAHFNALENLQTRTHRQGAGATMLIASVVELGLQVVVLSGIVW
                     VVTGTLNLAFLIAAVAMIMRFAEPMAMFISYTSVVELIASALQRIEQFMAIAPLPVAE
                     QSEMPERYDIRFDNVSYRYEEGDGHALNHVSLTFPAASMSALVGASGAGKTTVTKLLM
                     RYADPQQGQISIGGVDIRRLTPEQLNSLISVVFQDVWLFDDTLLANIRIACPQATRQE
                     VEEAARAAQCLEFISRLPQGWLTPMGEMGGQLSGGERQRISIARALLKNAPVVILDEP
                     TAALDIESELAVQKAIDNLVYNRTVIIIAHRLSTIAGAGNILVMEEGQVVEQGTHAQL
                     LSHHGRYQALWQAQMAARVWRDDGVSASGEWVHE"
     gene            complement(5681..7393)
                     /gene="ybtP"
                     /locus_tag="ACHOSC_RS04985"
                     /old_locus_tag="pgaptmp_000997"
     CDS             complement(5681..7393)
                     /gene="ybtP"
                     /locus_tag="ACHOSC_RS04985"
                     /old_locus_tag="pgaptmp_000997"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005227764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="yersiniabactin ABC transporter
                     ATP-binding/permease protein YbtP"
                     /protein_id="HGU4546980.1"
                     /translation="MALAGLAALTSLGALLFLVWSLRDIRATPDAIPAWPLGGVIGCV
                     VLTFVLRLQAFNTSHYAAFHLENILRSRLARKALQLPPGVLQQMGSGSVAKVMLDDVK
                     SLHIFVADSTPLYARAIIMPLATIVILFWLDWRLAIATLGVLAFGSVVLVLARQRSED
                     MAQRYHKAREQVSAAVIEFVQAMPVVRTFDSGSTSFLRYQRALEEWVDVLQTWYRKAG
                     FSARFSFSILNPLPTLFVLIWSGYGLLHYGSFDFIAWVAVLLLGSGMAEAVMPMMMLN
                     NLVAQTRLSIQRIYQVLAMPELSLPQSEQQPQEASITFEQVSFHYPQARTGAALQEVS
                     FHVPAGQIVALVGPSGAGKSTVARLLLRYADPDKGHIRIGGVDLRDMQTDTLMKQLSF
                     VFQDNFLFVDTIANNIRLGAPDTPLEAVIAAARVAQAHDFISALPEGYNTRVGERGVF
                     LSGGQRQRITIARALLQDRPILVLDEATAFADPENEAALIKALAAAMRGRTVIMVAHR
                     LSMVTQADVILLFSDGQLREMGNHTQLLAQGGLYQRLWQHYQQAQHWVPGGTQEEVVE
                     NERQ"
     gene            7650..8609
                     /gene="ybtA"
                     /locus_tag="ACHOSC_RS04990"
                     /old_locus_tag="pgaptmp_000998"
     CDS             7650..8609
                     /gene="ybtA"
                     /locus_tag="ACHOSC_RS04990"
                     /old_locus_tag="pgaptmp_000998"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005227765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="yersiniabactin transcriptional regulator YbtA"
                     /protein_id="HGU4546981.1"
                     /translation="MTESPQTQSEISIHQLVVGKPANDGNIPAQCELLRCSLQEGMDI
                     LLWRGHFARPETLQLHDDLGRINFSCILEGTSRFAIQGLRRHTDWELARNRHYITHTP
                     DCRGSASYCGRFESITLSFSPETLALWVPDISAVIKNKIDSHCCCQQQRCNAETHLTA
                     QALRHALMRMHDGFNHEQKPSTLWLQGQSLVMLSLVLDEHREDASCLSCHFNPMERQK
                     LLRAKDLLLADLTQAPGVAELARESGLSVLKIKRGFRVLFNNSVYGLFQAERMQEARR
                     RLANGNTSVMTVAADLGYANASHFSAAFQKQFGVTPSTFKRGM"
     gene            8800..14907
                     /gene="irp2"
                     /locus_tag="ACHOSC_RS04995"
                     /old_locus_tag="pgaptmp_000999"
     CDS             8800..14907
                     /gene="irp2"
                     /locus_tag="ACHOSC_RS04995"
                     /old_locus_tag="pgaptmp_000999"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005227766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:1904091 - non-ribosomal peptide synthetase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="yersiniabactin non-ribosomal peptide synthetase
                     HMWP2"
                     /protein_id="HGU4546982.1"
                     /translation="MISGAPSQDSLLPDNRHAADYQQLRERLIQELNLTPQQLHEESN
                     LIQAGLDSIRLMRWLHWFRKNGYRLTLRELYAAPTLAAWNQLMLSRSPENAEEETPPD
                     ESSWPNMTESTPFPLTPVQHAYLTGRMPGQTLGGVGCHLYQEFEGHCLTASQLEQAIT
                     TLLQRHPMLHIAFRPDGQQVWLPQPYWNGVTVHDLRHNDAESRQAYLDALRQRLSHRL
                     LRVEIGETFDFQLTLLPDNRHRLHVNIDLLIMDASSFTLFFDELNALLAGESLPAIDT
                     RYDFRSYLLHQQKINQPLRDVARAYWLAKASTLPPAPVLPLACEPATLREVRNTRRRM
                     IVPATRWHAFSNRAGEYGVTPTMALATCFSAVLARWGGLTRLLLNITLFDRQPLQPAV
                     GAMLADFTNILLLDTACDGDTVSNLARKNQLTFTEDWEHRHWSGVELLRELKRQQRYP
                     HGAPVVFTSNLGRSLYSSRAESPLGEPEWGISQTPQVWIDHLAFEHHGEVWLQWDSND
                     ALFPPALVETLFDAYCQLINQLCDDESAWQKPFADMMPASQRAIRERVNATGAPIPEG
                     LLHEGIFRIALQQPQALAVTDMRYQWNYHELTDYARRCAGRLIECGVQPGDNVAITMS
                     KGAGQLVAVLAVLLAGAVYVPVSLDQPAARREKIYADASVRLVLICQHDASAGSDDIP
                     ALAWQQAIEAEPIANPVVRAPTQPAYIIYTSGSTGTPKGVVISHRGALNTCCDINTRY
                     QVGPHDRVLALSALHFDLSVYDIFGVLRAGGALVMVMENQRRDPHAWCELIQRHQVTL
                     WNSVPALFDMLLTWCEGFADATPENLRAVMLSGDWIGLDLPARYRAFRPQGQFIAMGG
                     ATEASIWSNACEIHDVPAYWRSIPYGFPLTNQRYRVVDEQGRDCPDWVPGELWIGGIG
                     VAEGYFNDPLRSEQQFLTLPDERWYRTGDLGCYWPDGTIEFLGRRDKQVKVGGYRIEL
                     GEIESALSQLVGVKQATVLAIGEKEKTLAAYVVPQGEAFCVTDHRNPALPQAWHTLAG
                     TLPCCAISPEISAEQVADFLQHRLLKLKPGHTAGADPLPLMNSLAIQPRWQAVVERWL
                     AFLVTQRRLKPAAEGYQVCAGEEREDEHPHFSGHDLTLSQILRGARNELSLLNDAQWS
                     PESLAFNHPASAPYIQELATICQQLAQRLQRPVRLLEVGTRTGRAAESLLAQLNAGQI
                     EYVGLEQSQEMLLSARQRLAPWPGARLSLWNADTLAAHAHSADIIWLNNALHRLLPED
                     PGLLATLQQLAVPGALLYVMEFRQLTPSALLSTLLLTNGQPEALLHNSADWAALFSAA
                     GFNCQHGDEVAGLQRFLVQCPDRQVRRDPRQLQAALAGRLPGWMVPQRIVFLDALPLT
                     ANGKIDYQALKRRHTPEAENPAEADLPQGDIEKQVAALWQQLLSTGNVTRETDFFQQG
                     GDSLLATRLTGQLHQAGYEAQLSDLFNHPRLADFAATLRKTDVPVEQPFVHSPEDRYQ
                     PFALTDVQQAYLVGRQPGFALGGVGSHFFVEFEIADLDLTRLETVWNRLIARHDMLRA
                     IVRDGQQQVLEQTPPWVIPAHTLHTPEEALRVREKLAHQVLNPEVWPVFDLQVGYVDG
                     MPARLWLCLDNLLLDGLSMQILLAELEHGYRYPQQLLPPLPVTFRDYLQQPSLQSPNP
                     DSLAWWQAQLDDIPPAPALPLRCLPQEVETPRFARLNGALDSTRWHRLKKRAADAHLT
                     PSAVLLSVWSTVLSAWSAQPDFTLNLTLFDRRPLHPQINQILGDFTSLMLLSWHPGES
                     WLHSAQSLQQRLSQNLNHRDVSAIRVMRQLAQRQNVPAVPMPVVFTSALGFEQDNFLA
                     RRNLLKPVWGISQTPQVWLDHQIYESEGELRFNWDFVAALFPAGQVERQFEQYCALLN
                     RMAEDESGWQLPLAALVPPVKHAGQCAERSPRVCPEHSQPHIAADESTVSLICDAFRE
                     VVGESVTPAENFFEAGATSLNLVQLHVLLQRHEFSTLTLLDLFTHPSPAALADYLAGV
                     ATVEKTQRPRPVRRRQRRI"
     gene            14995..24477
                     /gene="irp1"
                     /locus_tag="ACHOSC_RS05000"
                     /old_locus_tag="pgaptmp_001000"
     CDS             14995..24477
                     /gene="irp1"
                     /locus_tag="ACHOSC_RS05000"
                     /old_locus_tag="pgaptmp_001000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005227767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="yersiniabactin polyketide synthase HMWP1"
                     /protein_id="HGU4546983.1"
                     /translation="MDNLRFSSAPTADSIDASIAQHYPDCEPVAVIGYACHFPESPDG
                     ETFWQNLLEGRECSRRFTREELLAVGLDAAIIDDPHYVNIGSVLDNADCFDATLFGYS
                     RQEAESMDPQQRLFLQAVWHALEHAGYAPGAVPHKTGVFASSRMSTYPGREALNVTEV
                     AQVKGLQSLMGNDKDYIATRAAYKLNLHGPALSVQTACSSSLVAVHLACESLRAGESD
                     MAVAGGVALSFPQQAGYRYQPGMIFSPDGHCRPFDASAEGTWAGNGLGCVVLRRLRDA
                     LLSGDPIISVILSSAVNNDGNRKVGYTAPSVAGQQAVIEEALMLAAIDDRQVGYIETH
                     GTGTPLGDAIEIEALRNVYAPRPQDQRCALGSVKSNMGHLDTAAGIAGLLKTVLAVSR
                     GQIPPLLNFHTPNPALKLEESPFTIPVSAQAWQDEMRYAGVSSFGIGGTNCHMIVASL
                     PDALNARLPNTDSGRKSTALLLSAASDSALRRLATDYAGALRENADASSLAFTALHAR
                     RLDLPFRLAAPLNRETAEALSAWAGEKSGALVYSGHGASGKQVWLFTGQGSHWHTMGQ
                     TMYQHSTAFADTLDRCFSACSEMLTPSLREAMFNPDSAQLDNMAWAQPAIVAFEIAMA
                     AHWRAEGLKPDFAIGHSVGEFAAAVVCGHYTIEQVMPLVCRRGALMQQCASGAMVAVF
                     ADEDTLMPLARQFELDLAANNGTQHTVFSGPEARLAVFCATLSQHDINYRRLSVTGAA
                     HSALLEPILDRFQDACAGLHAEPGQIPIISTLTADVIDESTLNQADYWRRHMRQPVRF
                     IQSIQVAHQLGARVFLEMGPDAQLVACGQREYRDNAYWIASARRNKEASDVLNQALLQ
                     LYAAGVALPWADLLAGDGQRIAAPCYPFDTERYWKERVSPACEPADAALSAGLEVASR
                     AATALDLPRLEALKQCATRLHAIYVDQLVQRCTGDAIENGVDAMTIMRRGRLLPRYQQ
                     LLQRLLNNCVVDGDYRCTDGRYVRARPIEHQQRESLLTELASYCEGFQAIPDTIARAG
                     DRLYEMMSGAEEPVAIIFPQSASDGVEVLYQEFSFGRYFNQIAAGVLRGIVQTRQPRQ
                     SLRILEVGGGTGGTTAWLLPELNGVPALEYHFTDISALFTRRAQQKFADYDFVKYSEL
                     DLEKEAQSQGFQAQSYDLIVAANVIHATRHIGRTLDNLRPLLKPGGRLLMREITQPMR
                     LFDFVFGPLVLPLQDLDAREGELFLTTAQWQQQCRHAGFSKVAWLPQDGSPTAGMSEH
                     IILATLPGQAVSAVTFTAPSEPVLGQALTDNGDYLADWSDCAGQPEQFNARWQEAWRL
                     LSQRHGDALPVEPPPVAAPEWLGKVRLSWQNEAFSRGQMRVEARHPAGEWLPLSPAAP
                     LPAPQTHYQWRWTPLNVASIDHPLTFSFSAGTLARSDELAQYGIIHDPHASSRLMIVE
                     ESEDTLALAEKVIAALTASAAGLIVVTRRAWRVEENEALSASHHALWALLRVAANEQP
                     ERLLAAIDLAENTPWETLHQGLSAVSLSQRWLAARGDTLWLPSLAPNTGCAAELPANV
                     FTGDSRWHLVTGAFGGLGRLAVNWLREKGARRIALLASHVDESWLRDVEGGQTRVCRC
                     DVGDAGQLATVLDDLAANGGIAGAIHAAGVLADAPLQELDDHQLAAVFAVKAQAASQL
                     LQTLRNHDGRYLILYSSAAATLGAPGQSAHALSCGYLDGLAQQFSTLDAPKTLSVAWG
                     AWGESGRAATPEMLATLASRGMGALSDAEGCWHLEQAVMRGAPWRLAMRVFTDKMPPL
                     QQALFNISATEKAATPVIPPADDNAFNGSLSDETAVMAWLKKRIAVQLRLSDPASLHP
                     NQDLLQLGMDSLLFLELSSDIQHYLGVRINAERAWQDLSPHGLTQLICSKPEATPAVS
                     QPEVLRHDADERYAPFPLTPIQHAYWLGRTHLIGYGGVACHVLFEWDKRHDEFDLAIL
                     EKAWNQLIARHDMLRMVVDADGQQRILATTPEYHIPRDDLRALSPEEQRIALEKRRHE
                     LSYRVLPADQWPLFELVVSEIDDCHYRLHMNLDLLQFDVQSFKVMMDDLAQVWRGETL
                     APLAITFRDYVMAEQARRKTSAWHDAWDYWQEKLPQLPLAPELPMVETPPETPHFTTF
                     KSTIGKTEWQAVKQRWQQQGVTPSAALLTLFAATLERWSRTTTFTLNLTFFNRQPIHP
                     QINQLIGDFTSVTLVDFNFSAPVTLQEQMQQTQQRLWQNMAHSEMNGVEVIRELGRLR
                     GSQRQPLMPVVFTSMLGMTLEGMTIDQAMSHLFGEPCYVFTQTPQVWLDHQVMESDGE
                     LMFSWYCMDNVLEPGAAEAMFNDYCAILQAVIAAPESLKTLASGIAGHIPRRRWPLNA
                     QADYDLRDIEQATLEYPGIRQARAEITEQGALTLDIVMADDPSPSAAMPDEHELTQLA
                     LPLPEQAQLDELEATWRWLEARALQGIAATLNRHGLFTTPEIAHRFSAIVQALSAQAS
                     HQRLLRQWLQCLTEREWLIREGESWRCRIPLSEIPEPQEACPQSQWSQALAQYLETCI
                     ARHDALFSGQCSPLELLFNEQHRVTDALYRDNPASACLNRYTAQIAALCSAERILEVG
                     AGTAATTAPVLKATRQSYHFTDVSAQFLNDARARFHDESQVSYALFDINQPLDFTAHP
                     EAGYDLIVAVNVLHDASHVVQTLRRLKLLLKAGGRLLIVEATERNSVFQLASVGFIEG
                     LSGYRDFRRRDEKPMLTRSAWQEVLVQAGFANELAWPAQESSPLRQHLLVARSPGVNR
                     PDKKAVSRYLQQRFGTGLPILQIRQREALFTPLHAPSDAPTEPAKPTPVAGGNSALEK
                     QVAELWQSLLSRPVARHHDFFELGGDSLMATRMVAQLNRRGIARANLQDLFNHSTLSD
                     FCAHLQAATSGEDNPIPLCQGDGEETLFVFHASDGDISAWLPLASALNRRVFGLQAKS
                     PQRFATLDQMIDEYVGCIRRQQPHGPYVLAGWSYGAFLAAGAAQRLYAKGKQVRMVLI
                     DPVCRQDFCCENRAALLRLLAEGETPLALPEHFDQQTPDSQLADFISLAKTAGMVSQN
                     LTLQAAETWLDNIAHLLRLLTEHTPGESVPVPCLMVYAAGRPARWTPAETELQGWINN
                     ADDAVIEASHWQIMMEAPHVQACAQHITRWLCATSTQPENTL"
     gene            24474..25574
                     /gene="ybtU"
                     /locus_tag="ACHOSC_RS05005"
                     /old_locus_tag="pgaptmp_001001"
     CDS             24474..25574
                     /gene="ybtU"
                     /locus_tag="ACHOSC_RS05005"
                     /old_locus_tag="pgaptmp_001001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005227768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="yersiniabactin biosynthesis oxidoreductase YbtU"
                     /protein_id="HGU4546984.1"
                     /translation="MMPSASPKQRVLIVGAKFGEMYLNAFMQPPEGLELVGLLAQGSA
                     RSRELAHAFGIPLYTSPEQITRMPDIACIVVRSTVAGGTGTQLARHFLTRGVHVIQEH
                     PLHPDDISSLQTLAQEQGCCYWVNTFYPHTRAGRTWLRDAQQLRRCLAKTPPVVHATT
                     SRQLLYSTLDLLLLALGVDAAAVECDMVGSFSDFHCLRLFWPEGEACLLLQRYLDPDD
                     PDMHSLIMHRLLLGWPEGHLSLEASYGPVIWSSSLYVADHQQNPHSLWRRPEILRDLP
                     GLTRSAAPLSWRDCCETVGPEGVSWLLHQLRSNLAGEHPPEACQSVHQIALSRLWQQI
                     LRKTGNAEIRRLTPPHHDRLAGFYNDDDKEAL"
     gene            25571..26374
                     /gene="ybtT"
                     /locus_tag="ACHOSC_RS05010"
                     /old_locus_tag="pgaptmp_001002"
     CDS             25571..26374
                     /gene="ybtT"
                     /locus_tag="ACHOSC_RS05010"
                     /old_locus_tag="pgaptmp_001002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002212799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="yersiniabactin biosynthesis thioesterase YbtT"
                     /protein_id="HGU4546985.1"
                     /translation="MTQSAMCIPLWPARNGNTAHLVMCPFAGGSSSAFRHWQAEQLAD
                     CALSLVTWPGRDRLRHLEPLRSITQLAALLANELEASVSPDTPLLLAGHSMGAQVAFE
                     TCRLLEQRGLAPQGLIISGCHAPHLHSERQLSHRDDADFIAELIDIGGCSPELRENQE
                     LMSLFLPLLRADFYATESYHYDSPDVCPPLRTPALLLCGSHDREASWQQVDAWRQWLS
                     HVTGPVVIDGDHFYPIQQARSFFTQIVRHFPHAFSAMTAWQKQPSTSER"
     gene            26378..27943
                     /gene="ybtE"
                     /locus_tag="ACHOSC_RS05015"
                     /old_locus_tag="pgaptmp_001003"
     CDS             26378..27943
                     /gene="ybtE"
                     /locus_tag="ACHOSC_RS05015"
                     /old_locus_tag="pgaptmp_001003"
                     /EC_number="2.7.7.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005227770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="yersiniabactin biosynthesis salycil-AMP ligase
                     YbtE"
                     /protein_id="HGU4546986.1"
                     /translation="MNSSFESLIEQYPLPIAEQLRHWAARYASRIAVVDAKGSLTYSA
                     LDAQVDELAAGLSSLGLRSGEHVIVQLPNDNAFVTLLFALLRLGVIPVLAMPSQRALD
                     IDALIELAQPVAYVIHGENHAELARQMAHKHACLRHVLVAGETMSDDFTPLFSLHGER
                     QPDVSTTALLLLSGGTTGTPKLIPRRHADYSYNFSASAELCGISQQSVYLAVLPVAHN
                     FPLACPGILGTLACGGKVVLTDSASCDEVMPLIAQERVTHVALVPALAQLWVQAREWE
                     DSDLSSLRVIQAGGARLDPTLAEQVIATFDCTLQQVFGMAEGLLCFTRLDDPHATILH
                     SQGRPLSPLDEICIVDQDENDVAPGETGQLLTRGPYTISGYYRAPAHNAQAFTAQGFY
                     RTGDNVRLDEVGNLHVEGRIKEQINRAGEKIAAAEVESALLRLAEVQDCAVVAAPDTL
                     LGERICAFIIAQQVPTDYQQLRQQLTRMGLSAWKIPDQIEFLDHWPLTAVGKIDKKRL
                     TALAVDRYRHSAQ"
     gene            28074..30095
                     /gene="fyuA"
                     /locus_tag="ACHOSC_RS05020"
                     /old_locus_tag="pgaptmp_001004"
     CDS             28074..30095
                     /gene="fyuA"
                     /locus_tag="ACHOSC_RS05020"
                     /old_locus_tag="pgaptmp_001004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005227771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="siderophore yersiniabactin receptor FyuA"
                     /protein_id="HGU4546987.1"
                     /translation="MKMTRLYPLALGGLLLPAIANAQTSQQDESTLVVTASKQSSRSA
                     SANNVSSTVVSAPELSDAGVTASDKLPRVLPGLNIENSGNMLFSTISLRGVSSAQDFY
                     NPAVTLYVDGVPQLSTNTIQALTDVQSVELLRGPQGTLYGKSAQGGIINIVTQQPDST
                     PRGYIEGGVSSRDSYRSKFNLSGPIEDGLLYGSVTLLRQVDDGDMINPATGSDDLGGT
                     RASIGNVKLRLAPDDQPWEMGFAASRECTRATQDAYVGWNDIKGRKLSISDGSPDPYM
                     RRCTDSQTLSGKYTTDDWVFNLISAWQQQHYSRTFPSGSLIVNMPQRWNQDVQELRAA
                     TLGDARTVDMVFGLYRQNTREKLNSAYDMPTMPYLSSTGYTTAETLAAYSDLTWHLTD
                     RFDIGGGVRFSHDKSSTQYHGSMLGNPFGDQGKSNDDQVLGQLSAGYMLTDDWRVYTR
                     VAQGYKPSGYNIVPTAGLDAKPFVAEKSINYELGTRYETADVTLQAATFYTHTKDMQL
                     YSGPDGMQTLSNAGKADATGVELEAKWRFAPGWSWDINGNVIRSEFTNDSELYHGNRV
                     PFVPRYGAGSSVNGVIDTRYGALMPRLAVNLVGPHYFDGDNQLRQGTYATLDSSLGWQ
                     ATERMNISVYVDNLFDRRYRTYGYMNGSSAVAQVNMGRTVGINTRIDFF"
BASE COUNT         6412 a         9210 c         8432 g         6140 t
ORIGIN      
        1 ccagtcagag gagccaattt tctgttttca tgcatccttg caaatcttta tctgatgttg
       61 attcaacagg ttagtgtgaa aactcttctc ggtgcatttt gattttaccc tatgcatcgg
      121 gaaaaattgg tggtcaaatc tgggatcagg ttagttcgat aatggagtga cccccatatg
      181 ttccttaccg acgcaaaaat ccgcaccctc aagccttctg ataaaccctt taaagtctcc
      241 gattctcacg gtctgtatct gctggtcaag ccgggtggct cccgccactg gtatctcaaa
      301 gctggaggtt gcgtcccgca cacggcagca cctgagtaac ataatgcgcc atgcggtcca
      361 tcaggagtta atcgatacga accctgcagc aaaccttggc ggcgtgacca cacctcctgt
      421 cagacggcac tatcctgccc tgccgctgga gcggctgcct gaactgcttg aacgtattgg
      481 ggcatatcat cagggccgtg aactgacccg gcatgccgtt ctgctgatgc tgcatgtgtt
      541 cattcgctcc agtgaactgc gtttcgcccg ctggtcagag attgatttca caaaccgagt
      601 ctggacgata cccgcgacgc gagaacccat tattggcgtg cgttattccg gccgcggggc
      661 aaaaatgcga atgccgcata tcgtccccct ctcagaacag tccatcgcca ttctgaaaca
      721 gattaaggat atcaccggta ataatgaact gatcttcccc ggcgaccata acccgtataa
      781 gccaatgtgt gaaaacacgg tcaataaggc actgcgggtg atgggttacg acacgaaaaa
      841 ggatatctgc ggtcacggct tccgggcaat ggcatgcagt gcgctgatgg aatcgggttt
      901 atgggcaaag gacgcagtag aacgccagat gagtcatcag gagcgcaata ccgtgcgcat
      961 ggcttatatt cataaggcag agcacctaga agcccgcaaa gcgatgatgc agtggtggtc
     1021 ggattatctg gaagcatgcc gagaatctta tgcgccgcct tatacaattg gtaaaaataa
     1081 gtttatccca tagtatacta acgaatccct cactctggca aattaccacc acctccacag
     1141 gaggtggttc tatatagact tttatcaact tcatgacgaa aaacacctat tttatcccaa
     1201 aagaagacaa tagagtcaca gaggaatcaa agagcgactc aattgtcttt ggacagtctg
     1261 gttgtgaggt tagaaaacag ttactcctac accattaaat agggcgcaat gctcgctaat
     1321 ttctcccggg tttctgtcag ttcgcgttcc ggcgtagatt gcgcgatgat tcccgccccc
     1381 gcctgtatcc agcagcgctg gctatcctga aatacggaac gcagaactag cgccgcatca
     1441 aagcgcgtat cgtccagcag caggattgcg ccggaataaa gctctcgcgg cgtcttctca
     1501 atttgcataa tcgcgttcag agcagcattt ttagggatac ctgaggcggt aatcgacgga
     1561 aacagcacgg taaacgcatc ccaggcatcc ttgttttccg aaagctgacc gctcacgcca
     1621 gaccccagat gctgaacgct gccgcgctgg cgaaccgaca ttaaatcttc aaccaccacg
     1681 ctgcccggct ggcaaacagc ctccagttca gcaatagctt ctttgacaga aaggatatgc
     1741 tcaagcacct ctttactgtc gtgcagcagt tctgcctctt tcgccttatt atgctccggg
     1801 tttcccatgc gatcgcgggt gcccgcaagc ggttcagtga ccactttatt gcccatcact
     1861 gacatcacca gttccgggct aaagcccagc gcttcgcgtc cttcctgacg gaacataaac
     1921 gagcgcacag gcgtgtttgc ctgccgcccg tataacagcg tggcgggcat atcaatccgc
     1981 gatggcaggg gaatggcgcg cgagacaatc actttgacat actcgccacg gcggatctcc
     2041 gcaacggcgc gcgcaacctg ttgtttatat gcctcaccat tgagggcggt atccaccgcc
     2101 agtggtgcgt tctgcgtcgt tgtaccggcc tcttttaccc actcgcacaa acgtcggcac
     2161 ccgtcggcgg agtccgcata aacggtgaca tttccctttt caaaaataag ttcttcacgg
     2221 ggaacggtta acgtcagcag cggccactcc ccggcgttaa aggcaatccc ccgcgcatgc
     2281 gccgcaaagt tgaacccaac ctgtccatat acccgacgtc ctttcacctg aggatgcgcc
     2341 ataaaacgac gcgcgcagtc ggcaatggaa tccaccgccc atttttgctc cccggcatca
     2401 tcaataaatg tcgcctgcgt tccgtcggca ttaatgtgca gccgtgcctg gcaccctttg
     2461 cctaaatacc aacagggttg acgctcatag acataacact cttcttctgc gaactggcgt
     2521 aaaacgccag aaatcgtcgg tagccattgt tcctctggta acgccagatg tagaaattca
     2581 ctgattttca tcccttacct ctctgtgtta ttcgcggatc aatcaattgc gcttgtcatc
     2641 agttctcttg ccgaatgcag ccggatataa gcaacccacg ccgccagcaa cgtgaatgcc
     2701 ccggcaaaca ggaagcaggc cgcatagcca aaatgttgag caacaacgcc accgataacg
     2761 cctgccagaa tggcgattgc cgcatcagta cattgaaaga gggtgaagtc gacaccggcc
     2821 tgcaaaggcg atgaaagcga catcagcgtg gcgtaaagac agaccagcgc gcaggccagc
     2881 gaaatggact ggacaatgac cagacactgg agaatctgca gcagcagatg acccggcgcc
     2941 atcatgagcg tcatcaccac cgccagcagc gcgatcccct ggacgccata agccgtcaac
     3001 agcgctctgc cgggtgaggt gtatttcatc agcaatccgc cagccagcgt tcctgctatg
     3061 cccgctgcaa tattgccgcc gctgaacaac gcgcccaatg cggacatgct caacccatga
     3121 tccaacaaca gcggtgccag aagaggcagc acaaaccgca tgcctgaatt cagcatcaat
     3181 accagcagta agcccaggcg cgcctgcttc ctccttagcg cataacctaa cgccggaaca
     3241 tgcaggatag tcgctgttcg cgtcggctcc gtaatgcgcc acagcgggag tgacagcgcc
     3301 ataatcagca ccgccatcat cagcatggcg acaggccagc cggatgccgc cgacagcatc
     3361 atgaacaccc cgccgccgca catcattccc agatagctgc cgccgacctg cacgctgttt
     3421 ccccagccgt aacccgtgcg ggtcagttga tcgacacaaa agccgtcgct ggcgatatca
     3481 atggtggagg cgaccgtgcc tgccaccata aacagaacaa ataacgcgac gatttgcgta
     3541 tcggggaatc ccccttctcg cctaaaccag ccaatcgctg cggcaatagt caggatcgcc
     3601 gctagcgcga cctgaccacg caggattaac atgcgggaac ggcgttcctg gctaccgggc
     3661 ggaagccgcc agcgctcgat ccacggcgcc caaataaact tcagcgccca gggcagcata
     3721 aacagggttg tcgcaccggc aagcgccagc gaaccgccag cagcgcgcac cagcgcgggt
     3781 aaggactgca tagacagcgc cgataccaga ctctgcgtca catagacgcc ggcaatagca
     3841 aaaatcaccc gcccggtcgt caacgtcagc ggtttcacat tcgactgaac atcactcatg
     3901 cacccactct ccagacgcgg aaaccccgtc gtcgcgccac acgcgcgcgg ccatttgcgc
     3961 ctgccacagc gcctgataac gcccatgatg tgagagcaat tgcgcatgag taccctgctc
     4021 aaccacctgt ccctcttcca tcaccagaat gtttccggcc ccggcgatgg tggataaacg
     4081 gtgagcgatg ataatcaccg tccggttgta aaccaggtta tcgatcgctt tttgcaccgc
     4141 cagctcgctt tcaatatcca gcgcggcagt cggttcatcg agaatgacga ctggcgcgtt
     4201 tttcaataac gctctggcaa tggaaatccg ctggcgctcg ccgcccgata gctggccgcc
     4261 catctctccc attggcgtca gccagccttg cggaagacgg gaaataaact caaggcactg
     4321 cgccgcgcgg gcggcctctt ctacctcctg ccgcgtcgcc tgtgggcacg cgatacggat
     4381 attcgccagc agcgtgtcat caaacagcca gacatcctgg aaaacaacag aaatcaggct
     4441 attgagctgt tccggcgtca ggcggcgaat atcgacgccg ccaatagaaa tctgcccttg
     4501 ctgcggatcg gcatagcgca ttaacagttt ggtgacggta gttttgcctg cgccggaggc
     4561 acccaccagc gcgctcatac tggctgccgg gaacgtcaaa gaaacatgat taagcgcgtg
     4621 gccgtcgcct tcttcgtagc gatagctgac gttgtcaaag cggatatcgt aacgttccgg
     4681 catctcgctt tgctctgcga cgggaagcgg tgctatcgcc ataaactgct caatccgttg
     4741 cagggcgctg gcgatcagtt ccacaaccga ggtgtagctg ataaacatcg ccatcggttc
     4801 tgcgaagcgc ataatcatcg cgacggcggc aatcaaaaag gcgaggttca gagtgcccgt
     4861 cactacccag acgatcccgg ataacaccac cacctgtagg cccaactcca cgacgctggc
     4921 gatcagcatc gtcgcgccag cgccctgacg gtgagtgcgg gtctgtaagt tttccagcgc
     4981 attgaaatgc gccagcagcg cccggctttt atcggcatcg ctgccgcagg tgcgcaatac
     5041 catcatcccc tgagcaaatt caacgatatc gccgctcaga cgctggtgcg cttcccccag
     5101 cgtctgcatt tgtcgccgca tcgccgggcg gcgccagtaa taaaacggca ccagcagagg
     5161 gaagatcagc aacatcacca gccccagccg ccagtctatc cacaatgtcg ccagcgacgc
     5221 cgtcagcggc gtgacaatgg tgagcagcaa aatattggct atcgcaataa cataattgag
     5281 gttttcatcc acgctgccca gcagcaaggc gttcatttca cccgccctgc cgcgctggag
     5341 cttctccagc ggcacgcggc gtaactgctc gccaagtcgc aggcgcaact catgggtagc
     5401 ctgcgccaga tgaccacggt attcaaagcc caggccatac cagcgtagca ccagcgtcac
     5461 aattgcggcg acgctgaagg ccatagccca gttaagaagt tgcggcgcgt ctccccgtaa
     5521 cagcgcatca atgatcggat aaagacaggc aaaggcgatc ccctgcatga atgccgccag
     5581 caacagcgcc agcatactgc gtcgcagcat cctggcctga ctgccaacgc tggagataag
     5641 ctggcgccag atgacgcgcc aggccaggtt atccgcagga ttattgtctt tcattttcca
     5701 ccacctcttc ctgtgttcca cccggcaccc aatgctgcgc ctgctgatag tgttgccaga
     5761 gccgttgata caggccgccc tgcgccaaca gttgcgtatg gttccccatt tccctgagct
     5821 gtccgtcgga aaacagcaat atcacatcgg cctgagtcac cattgagaga cgatgcgcga
     5881 ccatgatgac cgtccggcca cgcatggccg ccgcgagcgc cttgataagc gccgcttcgt
     5941 tttccgggtc agcaaacgcc gtcgcctcat cgagcaccag gatggggcga tcctgcaaaa
     6001 gcgcccgggc gatagtaatg cgctgccgct ggccgccgga gagaaatacc ccacgttccc
     6061 cgactcgagt gttgtaacct tctggcagag cgctaataaa atcatgggcc tgcgccactc
     6121 tggccgccgc tattaccgcc tccagcggcg tatccggcgc gcccagacga atgttattgg
     6181 ctatcgtgtc gacaaaaagg aagttgtcct gaaacacaaa cgagagttgc ttcatcaggg
     6241 tgtccgtctg catatcacgc agatccacgc cgccaatacg gatatggcct ttgtccgggt
     6301 cggcgtaacg cagcagcaag cgcgccacgg tgctttttcc ggcgccgctt ggcccgacca
     6361 gcgccacaat ttgcccggca ggcacatgaa agctcacctc ctgcaacgcg gcgccagtac
     6421 gcgcttgcgg ataatgaaag ctcacctgct caaaggtaat gctcgcctct tgcggctgct
     6481 gctcagactg cggcagcgat aactccggca tcgcgagaac ctgataaata cgctgaatgc
     6541 ttaaacgcgt ttgcgcgacc aggttattga gcatcatcat tggcattacg gcttcggcca
     6601 ttccgctgcc cagcagtaaa acggccaccc acgcgataaa atcgaaactg ccatagtgca
     6661 acaggccgta tcccgaccag atcaggacaa acagggtcgg gagaggattc agaatcgaga
     6721 aggaaaaacg cgctgaaaaa ccggctttgc gataccaggt ttggagcaca tcgacccact
     6781 cttcaagggc gcgttgatag cgcaaaaaac tggtgctgcc gctatcaaac gttctcacca
     6841 cgggcatggc ctgcacgaac tcaataaccg ccgcgctaac ctgctcgcgc gccttatgat
     6901 aacgctgagc catatcttcc gaacgctggc gggcgagcac gagaacaacc gatccaaacg
     6961 ccagtacccc cagcgtcgcg attgccagcc gccagtccag ccagaacaag ataacgattg
     7021 tcgccagcgg catgatgatc gcgcgggcat agagcggcgt gctgtcggcc acaaaaatat
     7081 gtaacgactt cacgtcatcc agcatcactt tcgccactga cccgctgccc atttgctgta
     7141 acacgccagg cggaagctgc aatgctttac gggctaacct gctgcgcaga atgttctcca
     7201 gatgaaaagc cgcgtaatga gaggtgttga acgcctgtaa gcgcagaaca aaagttaaga
     7261 ccacgcagcc gatcacgcca cccagcggcc aggccggaat agcgtcaggc gtcgcgcgaa
     7321 tgtcacgcag gctccacacc agaaaaagga gcgcccccag gctggttaat gccgccagcc
     7381 ccgccagcgc catagctaaa ataaccttcc tgcgaaccgg tgtaatcacc tgccagagag
     7441 gaaatcggga cagcgattca gtattggatg attgagatga catgggagta actgaatttc
     7501 ctgatgcatt tttacggacg ccaaaaacga caattcaggg tggcgttctg agaattaatg
     7561 tgaataataa ccattatcaa taactcatct accccattcg gttctattta tacccgcatt
     7621 ggtctaagcc acagggagat aaccaggtca tgacggagtc accgcaaacg caatctgaaa
     7681 tctctattca ccagttggtg gtcgggaaac ctgcaaacga tggcaatatt cctgctcagt
     7741 gtgaactgct gcgctgttcg ttgcaggaag gtatggatat tttgctctgg aggggccatt
     7801 ttgcgcggcc tgagacgttg cagctacacg acgatctggg caggattaac ttctcgtgca
     7861 tcctggaagg cacatcgcgc tttgcgattc agggactccg gcgccacacc gactgggagc
     7921 ttgcgcgcaa tcgccattac attacccata cgccagactg tcgcggcagc gcctcctatt
     7981 gtggccgatt cgagagcatt accctctcct tcagccctga aaccctcgcg ctgtgggtgc
     8041 cggatattag cgcggtcatt aagaataaga tcgactccca ctgctgttgc cagcagcagc
     8101 gttgtaatgc tgaaacccac ttaacggctc aggccttgcg tcacgcgctg atgcgcatgc
     8161 acgacggctt caatcatgaa caaaagccgt cgacgctatg gttgcagggg caaagtctgg
     8221 tcatgctcag tctggtgctg gatgaacatc gcgaagacgc cagttgcctc tcctgccact
     8281 tcaacccgat ggaacgccag aaactgctgc gggcgaagga tctgctgctg gctgatctga
     8341 cgcaagcgcc gggcgtcgcg gagctggcca gagagtccgg tctgagcgtt ctcaaaatta
     8401 agcgaggctt ccgcgttctg ttcaacaaca gcgtctatgg cctgtttcag gctgaacgaa
     8461 tgcaggaagc cagaagacgc ctggccaacg gcaatacctc cgtcatgacg gtggcggccg
     8521 atctgggcta tgccaacgcc agccatttct ctgccgcctt ccagaaacag tttggcgtaa
     8581 ctccttcgac ctttaaacgc gggatgtaac cggggtcgcg cccccctaaa ctcctccctg
     8641 acagaggccc ggatacccca tccgggcctc tgttctttcc gcctgttacc aacccgaaac
     8701 gggcataaat aaacccgttt cgggtagcat ccaattgtta ataattatta ttctcatatg
     8761 agtaatgctt ttcggtaaga cgtgccatca ggaggaagaa tgatttctgg cgcaccatct
     8821 caggattcgc tgttaccgga caaccgccac gcggctgatt accaacaatt acgcgagcgg
     8881 ctcatacagg aactgaattt aacgccgcag cagttacatg aagagagcaa cctgatccag
     8941 gccggtctgg attccataag attgatgaga tggttacact ggtttcgtaa aaatggctac
     9001 cgccttaccc ttcgcgagct gtatgccgcc cccacgctgg cggcatggaa ccagttaatg
     9061 ctcagccggt cgccggagaa cgcggaagaa gaaacgccgc ccgacgaatc atcctggccg
     9121 aacatgaccg aaagtacccc cttcccattg acgccagtac agcacgccta cctgacgggc
     9181 cgcatgccgg ggcagacgct tggcggcgtg ggttgccacc tgtatcagga gtttgaaggc
     9241 cattgtctga cggcgtcgca gctggagcag gccatcacga ccttgctgca acgccaccca
     9301 atgctgcata tcgcctttcg ccccgacggg cagcaggtct ggctaccgca accttactgg
     9361 aacggcgtca ccgttcatga tttacgccat aacgacgctg aaagccgcca ggcctatctg
     9421 gacgcactgc gccagcgcct gagccaccgt cttttacgcg tggaaatcgg cgaaacgttt
     9481 gattttcagc tgacgctctt gccggacaat cgccaccgcc tccatgtcaa tattgacctg
     9541 ctgattatgg atgcctccag ctttacgctt ttcttcgatg agcttaacgc cctgctggcc
     9601 ggagaatcgc tgccggctat cgacacccgc tatgatttcc gctcgtattt gctgcaccag
     9661 cagaagatca atcaaccact gagagacgtc gcgcgcgctt actggctggc gaaagcatcg
     9721 acgcttcccc ccgcgcccgt cttgccgctg gcctgcgaac ccgccacgct acgtgaagtc
     9781 cgtaataccc gacgccgcat gattgtcccg gcaacacgct ggcacgcctt tagcaaccgg
     9841 gccggcgagt atggcgtgac gccgacaatg gcactggcga cctgtttttc tgccgtgctg
     9901 gctcgctggg gcggcctgac gcgtctgctg cttaacatca ccttattcga ccgccagccg
     9961 ctgcaaccgg cggttggcgc gatgcttgcc gacttcacca atattcttct gctggatacc
    10021 gcctgcgatg gcgataccgt cagcaacctg gcgcgtaaaa accagctcac gtttacggag
    10081 gactgggagc atcgccactg gtccggcgtc gaattactcc gtgaactcaa acgccagcag
    10141 cgctaccccc acggcgcccc ggtggtattt accagcaatc tggggcgttc cctctacagc
    10201 agccgcgcag aatcgccgtt gggcgagccg gaatggggca tctcgcaaac gccgcaggtc
    10261 tggatagatc atctggcgtt cgagcatcac ggcgaggtct ggctacaatg ggacagcaac
    10321 gacgcgctgt tccctccggc gttagtcgaa acattgttcg acgcctactg ccagttgatt
    10381 aaccaactct gcgatgacga aagcgcctgg caaaagccgt tcgcagatat gatgcccgcc
    10441 agccagcgcg cgatacgcga acgggtcaac gccaccggcg cccccattcc cgaaggcttg
    10501 ctgcatgaag gcattttccg tatcgctctg caacagccgc aggcgctggc ggtaacggac
    10561 atgcgttatc agtggaatta tcatgagctg acagactatg cccgccgttg cgcgggcagg
    10621 ttaatcgagt gcggggttca gcccggcgat aatgtggcta tcacgatgtc gaaaggcgca
    10681 ggacaacttg ttgcggttct ggccgtcctg ctggccgggg cggtttacgt tccggtttcg
    10741 ctggatcagc ctgccgcacg gcgcgagaaa atctacgctg acgccagcgt ccggctggtg
    10801 ctcatttgtc agcacgacgc cagcgccggg tcagacgata ttcccgccct tgcctggcag
    10861 caggccattg aggcggagcc gatcgccaac ccggtagtac gcgcccccac gcaaccggcc
    10921 tacattatct acacctccgg ctctaccggt acgccgaaag gggtagtcat ttctcaccgg
    10981 ggagcgctta acacctgttg cgatatcaat acccgctatc aggttggccc gcatgacagg
    11041 gtgctggccc tctccgccct acattttgat ttatcggttt acgacatttt tggcgtactg
    11101 cgcgcgggcg gcgcgctggt gatggtgatg gaaaatcaac ggcgcgatcc tcacgcatgg
    11161 tgtgagctga tccagcgcca tcaggtcacg ctctggaaca gcgtcccggc gctgttcgat
    11221 atgctgctga cctggtgtga aggtttcgcc gacgccacgc cggaaaacct gcgcgcagtg
    11281 atgctttccg gcgactggat cgggcttgac ctccccgccc gttatcgggc cttccggcca
    11341 caaggacaat ttatcgcgat gggcggcgcc accgaggcgt ctatctggtc taacgcctgc
    11401 gaaattcacg acgtccccgc ctactggcgc tccatccctt acggttttcc gctaaccaac
    11461 caacgctacc gggtggtgga tgaacagggc cgggactgcc ctgactgggt gccgggtgaa
    11521 ttatggattg gcggcattgg ggtcgcggaa ggctatttca acgatcccct gcgtagcgag
    11581 cagcaatttt tgacgctccc ggacgagcgc tggtatcgca ccggcgatct cggctgctac
    11641 tggccagatg gcacaatcga gttcctcggt cgtcgcgaca agcaggtcaa agtcggagga
    11701 tatcgcatcg agctgggcga aatcgaaagc gcgctcagcc agctggtggg ggtgaaacaa
    11761 gcaaccgttc tggcgatcgg cgaaaaagaa aaaacgctgg cggcatacgt tgttcctcag
    11821 ggcgaggctt tttgcgttac cgatcatcgg aacccggcac tgccgcaggc gtggcacacg
    11881 cttgcgggaa cgttgccctg ttgcgccatc tcgccagaga tctccgcaga acaggtagcc
    11941 gatttccttc agcatcgcct gctaaaactg aagccgggtc acaccgctgg cgccgatcct
    12001 ctccccctga tgaactcact cgctatccag ccgcgctggc aggccgtggt ggaacgctgg
    12061 ttagcatttc tggtgacaca acggcgactg aagcccgctg ctgaaggtta tcaggtctgc
    12121 gctggtgaag aacgcgagga tgagcacccg cacttcagcg gacatgattt aacgttatcg
    12181 caaattcttc gcggtgcccg taacgaactg tcgttactga acgacgcgca gtggtcgccg
    12241 gaaagcctgg cctttaacca tccggccagc gccccgtata ttcaggaact ggcgacaatt
    12301 tgccaacagc ttgcacagcg cttacagcgc ccggtacgcc tgcttgaggt gggaacccgc
    12361 actggccgcg ccgcagaatc gctgttagca cagctcaacg ccggacagat tgagtatgtc
    12421 gggcttgagc agagccagga gatgctgctg agcgcccggc agaggctcgc cccctggcct
    12481 ggcgcccgtc tgtccctctg gaatgcagac acgctggcgg cgcacgctca ctcggcggac
    12541 attatctggc ttaataacgc cctgcatcgt ctgctgccgg aagatcccgg gctccttgcg
    12601 acattacaac agcttgccgt tcccggcgcg ctgctctacg tgatggagtt tcgccagtta
    12661 acgccgtccg ccctactcag cacgctcctg ttaaccaatg ggcagccgga ggccttgctg
    12721 cataacagcg ccgactgggc ggcattattt agcgcggccg gcttcaactg tcagcatggc
    12781 gatgaggtcg cggggttaca acgcttcctc gtacaatgtc ctgacaggca ggtgcgccgc
    12841 gatccccgtc aacttcaggc cgccctcgcc gggcgtctgc cggggtggat ggtgccgcaa
    12901 cggatcgtct tcctcgacgc cttaccgctg acagctaacg ggaaaattga ctaccaggcg
    12961 ctgaagcgtc gtcatacccc tgaagcggaa aacccggccg aagcggattt accccagggc
    13021 gacattgaaa aacaggttgc cgccctctgg cagcaactct tatcaactgg caatgtcacc
    13081 agagaaaccg acttcttcca gcaaggcggc gatagcctgc tggcgacccg tctgaccggg
    13141 caacttcatc aggcaggtta tgaagcgcaa ttaagcgacc tgtttaatca tccccggctg
    13201 gcggattttg ccgccacgct gcggaaaacc gacgtcccgg tcgaacaacc attcgtccac
    13261 tcccctgaag atcgctacca gccctttgcg cttaccgacg tgcagcaggc ttacctggtg
    13321 gggcgtcagc cgggctttgc cctgggcggc gtcggctcac atttctttgt tgaatttgaa
    13381 attgccgatc tggacctcac ccggctggag acggtctgga accgattaat cgcccgccac
    13441 gatatgctgc gcgccatcgt gcgtgatgga cagcaacagg tgctcgaaca gacgcccccc
    13501 tgggtgatac ccgcacacac cctccatacg cctgaagagg cgttgcgggt gcgcgaaaaa
    13561 ctggcgcatc aggtactcaa ccccgaagta tggccggtat tcgatctcca ggtcggatac
    13621 gtggacggga tgcctgcccg cctgtggctg tgtctggata acctgttgct tgacggtctg
    13681 agcatgcaga tcctgctggc ggagctggag cacggctacc gctacccgca acagctgctt
    13741 ccgccgctgc ccgtcacctt cagggattat ctgcaacaac cctcgctaca gtcgcccaat
    13801 ccagattctc tggcatggtg gcaggcgcag cttgatgata ttcctccggc gcctgcgttg
    13861 ccgctgcgct gcttgcctca ggaggttgaa acaccgcgct tcgcccgcct gaacggcgca
    13921 ctggacagca cgcgctggca tcggctgaaa aaacgggcgg ctgacgccca tctcaccccg
    13981 tcggccgtac tgttgtcggt gtggtcaacg gttctctctg catggagtgc acagcctgac
    14041 ttcacgctta accttacgct tttcgacagg cgaccgctgc acccgcaaat caaccagatt
    14101 ctgggcgatt tcacctcgct gatgctgctg agctggcatc ccggcgaaag ctggctgcac
    14161 agcgcgcagt cactacagca gcggctgagc cagaacctca accaccgcga tgtgtcagcc
    14221 atccgcgtga tgcgtcaact ggcgcaacgg caaaacgtgc cagccgttcc gatgcccgtc
    14281 gtctttacca gcgcgctggg ctttgagcag gataacttcc tcgcccggcg taatctgctc
    14341 aaaccggtct ggggcatctc ccagacgccg caggtctggc tcgatcacca gatttatgaa
    14401 tccgaaggcg aactgcgctt taactgggat tttgtcgccg cgctgtttcc tgccgggcag
    14461 gtggagcgcc agtttgaaca gtattgcgca ttgctaaacc gaatggccga ggatgaaagc
    14521 ggctggcaac tgccgctcgc cgcgctggtg cctcccgtta aacacgcagg gcaatgcgca
    14581 gagcgctcac cgcgcgtatg ccctgagcac tctcagccac acattgcggc ggacgagagc
    14641 accgtcagcc tgatttgcga cgccttccgc gaggtggttg gcgagtctgt cacgcccgca
    14701 gaaaacttct ttgaggcggg cgcaacgtcg ctgaatctgg tgcaactgca cgttttgtta
    14761 caacgtcacg aattttccac cctgacgttg cttgacctct tcacccaccc ttctcctgct
    14821 gccctggccg attatctggc cggcgtcgcc acggtggaga aaacacaacg acctcgccct
    14881 gttcgccgtc gtcagcggcg gatatagcgc gaagcaaact gattttcccc ggaacgccat
    14941 cgcgaacgca tggcgttcca ttgactttgt gaatcttagg aaacgggacc gattatggat
    15001 aacttgcgct tctcttctgc gccgacagca gattccattg atgcatcgat cgctcaacac
    15061 tacccggact gcgaacctgt cgcggttatc ggctacgcct gccattttcc tgaatcgccg
    15121 gatggcgaaa cgttctggca aaatctgctg gaaggtcgtg aatgcagccg acgctttacg
    15181 cgcgaagagc ttctggccgt cggtctggat gccgccatca ttgacgatcc tcattatgtc
    15241 aatatcggtt cggtgttaga caacgccgac tgcttcgacg ccaccctgtt tggctattcg
    15301 cgacaggaag cggagtcgat ggacccgcag cagcgcctgt ttttgcaggc ggtctggcat
    15361 gcgctggaac atgccggtta tgcccccggc gccgtccccc ataagaccgg cgttttcgcc
    15421 tcttcccgga tgagtaccta ccccggtcgc gaagcattga acgtgacaga agtcgcgcag
    15481 gtaaaaggtc tgcaatctct gatgggcaat gataaagact atattgccac ccgcgccgcg
    15541 tacaaactca acctgcacgg cccggcgtta tcggtacaga ccgcctgctc cagctcgctg
    15601 gttgccgtgc atctggcctg tgaaagcctg cgcgctggcg aatccgatat ggcggttgcc
    15661 ggcggcgtgg cgctctcttt cccccagcag gcaggctacc gctaccagcc cggaatgatt
    15721 ttctctcctg atggtcactg tcgtcccttt gacgcctcgg ctgagggcac ctgggccggt
    15781 aacggtctcg gctgcgtggt gctgcgtcgc ctgagagacg cgctgctgtc aggcgatccg
    15841 attatctcgg tgatcctctc cagcgcggtc aacaacgacg gcaacagaaa ggtcggctat
    15901 accgcccctt ccgtcgcagg gcaacaggca gtcatcgaag aggcgttaat gctggcggcc
    15961 atcgacgaca ggcaggtagg ttacattgaa acccacggca ccggcacacc gctgggcgac
    16021 gcgattgaaa ttgaagcgtt acgcaacgtc tatgcgcctc gcccgcagga tcagcgctgt
    16081 gcgctcggtt ccgtgaaaag taacatgggc catctggata ccgcggcggg cattgccgga
    16141 ctgctgaaaa ccgttctggc agtcagtcgc gggcaaattc ctcccttact gaattttcac
    16201 acccccaacc cggcgctgaa acttgaagag agccccttta ccataccggt gtcggcacag
    16261 gcatggcagg acgaaatgcg ctatgcgggc gtctcctcct ttggtattgg cggcaccaac
    16321 tgccatatga tcgtcgcctc gctgcccgac gcgctcaacg cgcgcctccc caatacggat
    16381 agcggcagaa aaagtaccgc gctgctgctc agcgccgcca gcgacagcgc gttgcggcgg
    16441 ctggcgacgg attatgccgg ggcgctgaga gagaatgcgg atgccagctc tctggccttc
    16501 acagccctgc acgcgcgccg tctcgatctc cccttccgcc tggcggcgcc attaaaccgt
    16561 gaaaccgccg aggcgctcag cgcctgggcc ggtgagaaat cgggggcgct ggtttacagc
    16621 ggccacggcg ccagcggcaa gcaggtgtgg ctgtttaccg gccagggctc gcactggcac
    16681 actatgggcc aaacgatgta ccagcactca acggcgtttg ccgacacgct ggatcgctgt
    16741 ttttccgcct gtagcgaaat gctcacgcca tcactgcgcg aagcgatgtt taaccccgat
    16801 tcggcgcagc tggacaatat ggcctgggcg cagccggcga ttgtcgcgtt tgaaatcgcg
    16861 atggcggcgc actggcgtgc tgaaggactg aagccagact tcgccattgg gcattccgtc
    16921 ggtgaatttg ccgctgccgt tgtctgcgga cactatacga ttgaacaggt catgccactg
    16981 gtttgtcggc gcggcgcgct aatgcagcag tgcgcaagcg gcgcgatggt ggcggtattt
    17041 gcagacgaag acacgctgat gccgctggct cgccagtttg agctggatct cgccgccaac
    17101 aacggtacgc aacatacggt attttccggg ccggaagccc gtctcgcggt attttgcgcc
    17161 acgctctcgc agcatgacat taactatcgt cgcctgagcg taaccggtgc ggcgcactcc
    17221 gctttactgg agccgatact cgatcggttc caggacgcct gcgcgggact gcacgcggag
    17281 ccggggcaaa taccgattat ttccacgctc accgccgacg tcattgatga gtcaacgctc
    17341 aaccaggcgg attactggcg ccgacacatg cgccagccgg tgcgttttat ccagagtatt
    17401 caggtggcgc atcagctcgg cgcccgcgtt tttctggaga tggggcccga tgcccagttg
    17461 gttgcttgcg ggcagcgcga ataccgcgat aacgcatact ggatagccag cgcccggcgt
    17521 aacaaagagg cgagcgatgt cctcaatcag gccctgctcc agctttacgc tgccggcgtc
    17581 gccctaccgt gggccgacct gctggcgggc gatggacaac gtatcgctgc gccatgttat
    17641 ccgtttgata ctgaacgtta ctggaaagag cgcgtctccc cggcctgcga gcctgccgac
    17701 gcagcgctgt ctgccgggct ggaggtggcg agtcgcgccg cgacagcgct cgatctccct
    17761 cgcctggaag cgcttaaaca gtgcgccacg cgactgcacg ccatctacgt cgatcaactg
    17821 gtacaacgct gtaccggcga tgccattgag aacggcgtgg acgccatgac catcatgcgc
    17881 cgtggacgtc tgctgccccg ctaccagcag ctactccagc gcctgctgaa taactgcgtg
    17941 gtcgacggcg attaccgctg caccgacggg cgatacgtcc gcgcccgccc cattgaacat
    18001 caacagcggg aatcactgct gacggaactt gccagttatt gtgaaggttt tcaggctatt
    18061 cccgacacca tcgcccgtgc cggcgatcgg ttatatgaaa tgatgagcgg cgcggaagaa
    18121 ccggtggcga ttatcttccc gcaaagcgcc tccgacggcg tggaagtgct gtatcaggaa
    18181 ttcagctttg gccgctattt caaccaaatc gccgccgggg tattacgcgg cattgtccag
    18241 acgcgtcagc cccgccagtc gttgcgtatt cttgaagttg gcggcggaac cggcggcacc
    18301 accgcgtggc tgctgccgga actcaacggc gttccggcac tggagtacca tttcaccgat
    18361 atctcggcgc tgttcacccg tcgcgcccag cagaaattcg ccgactatga ttttgtgaag
    18421 tatagcgagc tggatctcga aaaagaggcg cagtctcagg gtttccaggc acagtcttac
    18481 gatcttatcg tggcagcgaa cgtgattcac gccacccgcc atattggccg cacgctcgat
    18541 aatctgcgcc ccctgctcaa gccgggcggg cgcctgctga tgcgcgaaat cacccagcca
    18601 atgcgtctgt ttgacttcgt tttcggcccg ctggttcttc cgctacagga tctcgacgcc
    18661 cgcgaaggtg agttattcct caccaccgct cagtggcaac aacagtgccg ccacgccgga
    18721 ttcagcaaag tggcgtggct accgcaggat ggcagcccga ccgccgggat gagcgaacat
    18781 atcattctcg ccacgctgcc cggtcaggcg gttagcgccg taacattcac cgcgccatca
    18841 gaacccgtgt tggggcaggc gctgacggat aacggtgatt atctcgccga ctggtctgat
    18901 tgcgcaggtc agcccgaaca gtttaacgcc cgctggcagg aggcctggcg tctgctttca
    18961 cagcgtcatg gcgacgctct gcctgtggaa ccgccccccg tcgccgcccc ggagtggctg
    19021 gggaaggttc gcttaagctg gcaaaacgaa gccttttccc gcggtcagat gcgcgttgaa
    19081 gcccgtcatc ctgctggcga gtggctgccg ctatcgcccg ccgcgcctct tcctgcgccg
    19141 caaacgcatt atcaatggcg ctggacgccc ctcaacgtcg ccagcattga ccatccgctt
    19201 acctttagct tcagcgccgg tacgcttgcg cgcagcgacg agctggcgca atacggcatc
    19261 attcacgatc cgcacgcctc ttcacgactg atgattgttg aggagagcga ggatacgctg
    19321 gccttagcgg agaaagtgat agcagcgctc accgccagcg cagccggatt gattgtggtt
    19381 actcgccgcg cgtggcgagt cgaggaaaat gaagcactct ctgcatccca tcacgcgcta
    19441 tgggccttgc ttcgcgtcgc ggccaacgaa cagccggaac ggttgcttgc cgccatcgat
    19501 ctcgccgaaa acaccccgtg ggaaacgctg catcaagggt tgagcgcagt ctcactatca
    19561 cagcgctggc tcgccgcacg gggtgacacc ctttggctcc cttcactggc gcccaatacg
    19621 ggatgcgcag ctgaattacc ggcaaacgtg tttaccggcg atagccgctg gcatctggtg
    19681 accggagcgt ttggcggatt aggccgcctt gccgtgaact ggctcagaga aaaaggggcg
    19741 cgacgcatcg ccctgctggc atcgcacgtg gatgagtcat ggctacgcga cgtggagggc
    19801 gggcagacgc gcgtctgccg ttgtgatgtg ggcgatgccg ggcaactggc cacggttctt
    19861 gacgatctgg cggccaacgg cggcattgcc ggagcgattc atgccgctgg cgtattggct
    19921 gacgcgccct tgcaggagct tgatgaccac cagctggctg ccgttttcgc ggtaaaagcg
    19981 caggcggcaa gccagctgtt gcaaaccctg cgcaaccacg acggacgcta tcttattctc
    20041 tactcttccg ctgccgccac cctcggcgcg ccgggtcaga gcgcccatgc gctgtcctgc
    20101 ggctacctgg acgggctggc ccagcagttt tccacccttg atgcgccgaa aacgctctct
    20161 gtcgcctggg gcgcatgggg agaaagcggt cgggcggcca cgccggaaat gctggcgacg
    20221 ctcgccagcc gaggtatggg cgcgttaagc gatgccgaag gctgctggca cctggaacag
    20281 gcggtgatgc gcggcgcccc gtggcgactg gcgatgcgcg tttttaccga caaaatgccc
    20341 ccgttacaac aggctctgtt taacatcagc gccacagaaa aagccgcaac gccggtcatt
    20401 cctcctgctg atgacaacgc ctttaacggc agcctgagcg atgaaacagc ggtgatggca
    20461 tggctgaaaa agcggattgc ggttcagcta aggctgagcg atccggcgtc actgcatcca
    20521 aaccaggatc tgttgcaact cggcatggac tcgctgctct tccttgaact cagtagcgat
    20581 attcagcact acctgggcgt acgcatcaat gcggaacggg cgtggcagga tctgtctcct
    20641 catggactca cgcagcttat ctgttctaag ccagaggcga cgcctgccgt ttcgcagccg
    20701 gaagtgttgc ggcacgacgc cgacgagcgt tatgcgccct tccctttgac gcccattcag
    20761 cacgcctact ggctggggcg aacccacctc attggctatg gcggcgtcgc ctgtcacgtc
    20821 ctgtttgagt gggataaacg ccacgatgag ttcgatctag ccatactgga gaaagcatgg
    20881 aaccagctca tcgcacgcca cgatatgttg cgtatggtgg ttgatgccga cgggcagcag
    20941 cgaatcctgg cgacaacgcc ggagtatcac atcccgcgtg acgatctgcg cgcgctttcc
    21001 ccggaagaac agcgcatcgc gctggaaaaa cggcggcatg aactgagcta tcgcgttttg
    21061 cctgccgacc agtggcctct ttttgagctg gtggtcagcg aaatcgacga ttgccattac
    21121 cgtctgcata tgaacctcga ccttttgcag tttgatgtgc agagttttaa agtcatgatg
    21181 gacgacctgg cgcaggtctg gcgcggtgaa acgctggcgc cgctcgctat taccttccgt
    21241 gattatgtga tggctgaaca ggcgcgccga aagacatcgg catggcacga tgcctgggat
    21301 tactggcagg aaaaactgcc gcaactgccc ttagcgccag agctgccgat ggttgagacg
    21361 cccccggaaa cgccacactt caccaccttc aaatcgacga tcggcaagac agaatggcag
    21421 gccgtgaaac agcgctggca gcagcaaggc gtcacaccgt ctgccgcgct gctcacgctg
    21481 tttgccgcca cccttgagcg ctggagccgt accacaacat ttacgctgaa cctgacgttc
    21541 ttcaatcgcc agccgatcca tccgcaaatc aaccagttga ttggtgattt tacctccgtc
    21601 acgctggttg attttaactt ctcagcgcca gtgacgttgc aagagcagat gcaacagacc
    21661 caacagcgcc tctggcaaaa tatggcgcac agtgaaatga acggtgttga ggtgatccgt
    21721 gagctgggcc gcctgcgcgg atcacaacgt caaccgctga tgccggtagt gtttaccagt
    21781 atgctgggga tgacgctgga aggcatgact atcgatcagg cgatgagcca tctgttcggc
    21841 gaaccctgct atgtattcac gcaaacgccg caggtctggc tggatcatca ggtcatggag
    21901 agcgacggcg agttgatgtt tagctggtac tgcatggaca acgtgctgga acccggcgct
    21961 gccgaggcga tgtttaatga ctattgcgcc atcctgcaag ccgtcatcgc cgcccctgaa
    22021 agcctgaaga ctctcgccag cggcatcgcc gggcacattc cccgccgacg ctggccgctg
    22081 aacgcgcagg cggactacga cctgcgggat attgagcagg cgacgctcga ataccccggc
    22141 atccggcagg ccagagcgga aataaccgaa cagggcgcgt tgacgctgga tatcgtgatg
    22201 gccgacgatc cttcgccatc agcggcgatg cctgatgagc acgaacttac ccaactggcg
    22261 ctgccgttgc ctgagcaggc gcagcttgat gagctggagg cgacctggcg ctggctggag
    22321 gcgcgtgcgc tacaggggat cgcggctacg ctaaatcgtc acggcctgtt taccacgccg
    22381 gagatcgccc atcgctttag cgcaatagta caggcgctgt ccgcgcaagc gtctcaccag
    22441 cgtctgctgc gccagtggct acagtgtctg acggaaagag agtggttaat ccgcgaaggt
    22501 gaaagctggc gctgccgcat tccgctcagc gagattcctg agcctcagga agcgtgcccg
    22561 caaagccaat ggagccaggc gctggcgcag tatctggaaa cctgcatcgc ccggcacgac
    22621 gccctcttct ccgggcagtg ttctccgctg gaattgctgt tcaacgagca gcatcgcgtt
    22681 accgacgcgc tgtatcgcga caaccccgcc agcgcctgtc tgaatcgcta taccgcgcag
    22741 attgccgcct tgtgcagcgc agaacggatt ctggaggttg gcgccggaac cgcagccact
    22801 accgcgccgg tgctgaaggc cacgcggcag tcgtaccact tcacggacgt ctccgcgcag
    22861 ttcctcaatg acgccagagc ccgtttccat gatgaatcgc aggtgtctta tgccttgttc
    22921 gacatcaacc agccgctgga tttcaccgcc cacccggagg cgggttacga cctgatcgtt
    22981 gccgtcaatg tgctccacga cgccagccat gtcgtccaga cgttgcgcag attaaaactg
    23041 ttgctgaaag ccggcggacg tttgctgatc gttgaagcga cggagcgaaa cagcgtattc
    23101 cagctggcga gcgtgggctt tattgaggga ttaagcggat accgcgattt ccgccgccgg
    23161 gatgagaaac cgatgctcac ccgctccgca tggcaggagg ttctcgttca ggccgggttt
    23221 gcaaacgagc tggcgtggcc cgcgcaggaa tcgtcgccgc tgcgccagca tctgctggta
    23281 gcgcgttcgc ctggcgtaaa tcgcccggat aaaaaagccg tgagccgcta tttacagcag
    23341 cgctttggca ccggtctgcc cattttacag atccggcaaa gagaagcgtt atttacgccg
    23401 ctgcatgccc cgtctgatgc gccgactgag ccagccaaac ccacgccagt tgccgggggg
    23461 aattcggcgc tggaaaaaca ggtggctgaa ctctggcaat cgctgctgtc tcgccccgtg
    23521 gcaaggcatc acgacttttt cgaactgggc ggcgacagcc tgatggcgac aaggatggtc
    23581 gcgcagctaa accggagagg gattgccagg gctaaccttc aggatctgtt caaccattcg
    23641 acgctgagcg acttctgcgc ccatctacag gcggctacgt caggagagga caacccgata
    23701 cccctttgcc agggcgacgg tgaggaaacc ctgtttgtct tccacgcttc agacggcgat
    23761 atcagcgcct ggctgccgct cgctagcgcg ttgaacaggc gcgttttcgg cctgcaagca
    23821 aaatcgccgc agcgctttgc tacgctcgac cagatgatcg atgagtatgt cgggtgcatc
    23881 cgtcgtcagc agcctcacgg cccttatgtg ctggcgggtt ggtcgtatgg cgcgtttctt
    23941 gcggcgggcg ccgcacagcg cctgtacgcc aaaggcaagc aggttcggat ggtgttaatc
    24001 gatcccgtgt gccgacagga tttctgttgc gaaaaccggg cggccctgct gcgcctgtta
    24061 gccgaaggag aaacgcctct ggcactgccc gaacatttcg accagcagac gcccgacagc
    24121 cagcttgccg actttatcag cctcgctaaa acggccggta tggtgtcgca aaacctgacg
    24181 ctgcaagcgg cagaaacgtg gctcgacaac atcgcgcatc tgctgcgttt actgactgag
    24241 catacgccgg gcgaaagcgt tccggtcccc tgtctcatgg tgtatgccgc cgggagaccc
    24301 gcgcgctgga cgccagcaga aaccgagttg cagggctgga taaacaacgc cgacgacgct
    24361 gtgattgaag ccagccactg gcaaatcatg atggaagccc cccacgttca ggcttgtgcg
    24421 caacacatta cgcgctggct ttgcgcaacc tcaacgcaac cggagaacac gttatgatgc
    24481 cgtccgcctc cccaaaacaa cgcgtactga ttgtgggcgc caaatttggc gaaatgtacc
    24541 tgaatgcctt tatgcagccc ccggaggggc tggaactggt cggcctgctg gcgcagggaa
    24601 gcgcccgttc aagagagctg gctcatgcgt ttggcattcc gctgtatacc tcgccggaac
    24661 agataaccag gatgccggat atcgcctgta ttgtcgtgcg ttcgaccgtc gccggaggaa
    24721 ccggtacgca gcttgccaga cactttctga cgcgcggcgt acacgtcatt caggagcatc
    24781 ccctccaccc ggatgacatc agttccttac aaacgctggc gcaggaacag ggctgctgct
    24841 attgggtaaa cacgttttat ccgcatacgc gcgcgggacg cacatggctt cgcgatgcgc
    24901 agcagctgcg tcgctgcctg gctaagacgc cgccggtagt ccacgccacc accagccgac
    24961 agttgctcta ctccactctg gatttgttgt tgctggctct gggcgttgat gccgccgccg
    25021 tggagtgcga catggttggc agctttagcg attttcactg tctgagactc ttctggccgg
    25081 agggagaagc ctgcctgctg cttcagcgct atctcgatcc tgacgatcca gatatgcata
    25141 gccttatcat gcaccgcctg ctgctgggct ggccggaagg tcatctttcg ctggaggcga
    25201 gctacggccc cgtcatctgg tcatccagtc tgtatgtcgc agaccatcag cagaaccccc
    25261 acagtctctg gcgcagaccg gagatcctgc gcgatctgcc gggtctgacg cgcagcgcgg
    25321 cgcccctgag ctggcgcgac tgctgcgaaa ccgtcgggcc ggagggcgtc agctggttgc
    25381 tacaccagtt gcgcagcaat ctggcgggcg aacatccacc agaggcctgc caaagcgtac
    25441 atcagatcgc gttatcacgt ttatggcagc agattttacg taagaccggc aatgcggaga
    25501 tcagacgtct gacgccgccg caccacgacc ggttagccgg tttctacaac gatgatgata
    25561 aggaggcgct gtgacgcaat ctgcaatgtg catcccgctg tggcccgccc ggaacggcaa
    25621 tactgcgcat ctggtcatgt gccctttcgc tggcggcagc agtagcgcgt ttcgccactg
    25681 gcaagctgag caactggctg attgcgcgct ttctctggtg acctggccgg ggcgcgatcg
    25741 ccttcgccat ctggaaccgc tcagaagcat tacacaactg gcggcactgc tggcgaacga
    25801 gctggaagca tccgtatcgc ctgacacgcc gcttttactc gccgggcaca gcatgggggc
    25861 gcaggtggcg tttgaaacct gccgacttct ggagcaacgg gggcttgcgc cacaaggact
    25921 gattatttcc gggtgccatg ccccgcatct gcattctgaa cgccagctca gccatcgcga
    25981 tgatgccgac tttatcgctg agctgataga cattggcgga tgttctcctg aactgcggga
    26041 aaaccaggaa ttaatgtcgc tgtttcttcc tcttctgcgc gctgattttt acgccaccga
    26101 gagctatcac tacgactcgc ccgacgtctg tccgccgctg cgcacgcctg cgctgttatt
    26161 gtgcggcagc cacgatcgcg aagcctcctg gcagcaggtc gatgcctggc gtcagtggct
    26221 gagccacgtt acaggcccgg tggtgattga cggcgatcac ttctatccca ttcaacaagc
    26281 ccggtccttt tttacgcaga ttgtccgcca ttttccccac gcattttctg caatgaccgc
    26341 gtggcaaaaa cagcccagta cttcagaaag gtgacgcatg aattcttcct ttgaatctct
    26401 gattgaacag tatcccttac ccattgccga acagttgcgc cactgggcgg cccgttatgc
    26461 ctcgcgaatt gccgtcgttg atgcaaaggg gtcgttaacc tacagcgcgc ttgatgcaca
    26521 agttgacgaa cttgccgcag gtctgtcatc actgggtttg cgttcggggg agcatgtaat
    26581 tgtgcagctt cccaacgaca acgcgtttgt taccctgctg ttcgccttgt taagactggg
    26641 cgttatcccc gtgctggcga tgccctcgca acgggcgctg gatatcgacg cgctgattga
    26701 gctggcgcaa cccgtcgctt acgttattca cggggaaaac cacgcagagc tggcccgaca
    26761 gatggcacac aaacacgcct gcttgcgtca tgttctggtc gctggagaga ccatgagcga
    26821 cgattttacg ccgctcttct cccttcacgg tgagcgacag cctgatgttt ccaccaccgc
    26881 gttgttgttg ctctcaggcg gcacaaccgg cacgcccaaa ctcatcccgc gccgacatgc
    26941 cgactatagc tataacttca gcgcttctgc tgaactgtgc ggcatcagcc aacagagcgt
    27001 gtatctcgcc gtcctcccgg tggcgcataa ctttccgctg gcctgccccg gtattctggg
    27061 aacgcttgcc tgcggcggaa aagtggtgct gaccgacagc gccagctgtg atgaggtgat
    27121 gcctttaatc gcgcaggaaa gagtgactca cgtcgccctg gttccggcgc tggcgcaatt
    27181 atgggtgcag gccagggagt gggaagacag cgacctttcg tcgctgcgcg tcattcaggc
    27241 aggcggcgcc cggctcgacc cgacgcttgc tgagcaggtt atcgccacct ttgactgtac
    27301 cctgcaacag gttttcggta tggcggaagg cctgctctgt tttacccggc tggacgatcc
    27361 gcatgccacc attctccaca gccaggggcg cccgttgtcc cctctggatg aaatctgcat
    27421 cgttgatcaa gacgagaacg acgtcgcgcc gggcgaaacc gggcaattgt taacgcgcgg
    27481 cccttatacc atttcgggct attaccgcgc ccctgcccac aacgcgcagg cctttaccgc
    27541 gcaagggttt taccgcacag gcgacaatgt caggctggat gaggtgggga acctgcacgt
    27601 tgagggacgc ataaaagagc agatcaaccg cgccggagaa aaaatagccg cggctgaagt
    27661 ggaatcggca ctgctgcgtt tagcggaagt gcaagattgc gcggtggtcg ccgcgccgga
    27721 cacgctgctt ggcgagcgga tttgcgcgtt tatcatcgcg cagcaggtgc caactgacta
    27781 tcagcagttg cgtcaacaac tgacccgtat ggggctcagc gcgtggaaaa ttcctgacca
    27841 aatcgagttt ctggaccact ggccgctcac cgccgtcggc aagatagaca aaaaacgcct
    27901 gacggctctc gccgtcgacc gttatcgcca ttctgcccaa taagcgcaaa ccgacccgaa
    27961 acaggttgaa ataaacccgt ttcgggtagc accactatta gaaatagtta tcattttcaa
    28021 ttcaccattg tcggtatttt tggcgtttcg ccgtcttaca gggactcaca acaatgaaaa
    28081 tgacacggct ttatcctctg gccttggggg gattattgct ccccgccatt gctaatgccc
    28141 agacttcaca gcaagacgaa agcacgctgg tggttaccgc cagtaaacaa tcttcccgct
    28201 cggcatcagc caacaacgtc tcgtctactg ttgtcagcgc gccggaatta agcgacgccg
    28261 gcgtcaccgc cagcgacaaa ctccccagag tcttgcccgg gctcaatatt gaaaatagcg
    28321 gcaacatgct tttttcgacg atctcgctac gcggcgtctc ttcagcgcag gacttctata
    28381 accccgccgt caccctgtat gtcgatggcg tccctcagct ttccaccaac accatccagg
    28441 cgcttaccga tgtgcaaagc gtggagttgc tgcgaggccc acagggaacg ttatatggca
    28501 aaagcgctca gggcgggatc atcaacatcg tcacccagca gccggacagc acgccgcgcg
    28561 gctatattga aggcggcgtc agtagccgcg acagttatcg aagtaagttc aacctgagcg
    28621 gccccattga ggatggcctg ctgtacggca gcgtcaccct gttacgccag gttgatgacg
    28681 gcgacatgat taaccccgcg acgggaagcg atgacttagg cggcacccgc gccagcatag
    28741 ggaatgtgaa actgcgtctg gcgccggacg atcagccctg ggaaatgggc tttgccgcct
    28801 cacgcgaatg tactcgcgcc acccaggatg cctatgtggg atggaatgat attaagggcc
    28861 gtaagctgtc gatcagcgat ggttcaccag acccgtacat gcggcgctgc actgacagcc
    28921 aaaccctgag tgggaaatac accaccgatg actgggtttt caacctgatc agcgcctggc
    28981 agcagcagca ttattcgcgc accttccctt ccggttcgtt aatcgtcaat atgcctcagc
    29041 gctggaatca ggatgtgcag gagctgcgcg ccgcaaccct gggcgatgcg cgtaccgttg
    29101 atatggtgtt tgggctgtac cggcagaaca cccgcgagaa gttaaattca gcctacgaca
    29161 tgccgacaat gccttattta agcagtaccg gctataccac cgctgaaacg ctggccgcat
    29221 acagtgacct gacctggcat ttaaccgatc gttttgatat cggcggcggc gtgcgcttct
    29281 cgcatgataa atccagtaca caatatcacg gcagcatgct cggcaacccg tttggcgacc
    29341 agggtaagag caatgacgat caggtgctcg ggcagctatc cgcaggctat atgctgaccg
    29401 atgactggag agtgtatacc cgtgtagccc agggatataa accttccggg tacaacatcg
    29461 tgcctactgc gggtcttgat gccaaaccgt tcgtcgccga gaaatccatc aactatgaac
    29521 ttggcacccg ctacgaaacc gctgacgtca cgctgcaagc cgcgacgttt tatacccaca
    29581 ccaaagacat gcagctttac tctggcccgg acgggatgca gacattaagc aatgcgggta
    29641 aagccgacgc caccggcgtt gagcttgaag cgaagtggcg gtttgcgcca ggctggtcat
    29701 gggatatcaa tggcaacgtg atccgttccg aattcaccaa tgacagtgag ttgtatcacg
    29761 gtaaccgggt gccgttcgta ccacgttatg gcgcgggaag cagcgtgaac ggcgtgattg
    29821 atacgcgcta tggcgcactg atgccccgac tggcggttaa tctggtcggg ccgcattatt
    29881 tcgatggcga caaccagttg cggcaaggca cctatgccac cctggacagc agcctgggct
    29941 ggcaggcgac tgaacggatg aacatttccg tctatgtcga taacctgttc gaccgtcgtt
    30001 accgtaccta tggctacatg aacggcagca gcgccgtcgc gcaggtcaat atgggtcgca
    30061 ccgtcggtat caatacgcga attgatttct tctgattatt gtaaaaggga taccgaaaag
    30121 gtatcccttt tacaccacta gttaaaacca gtaactcagc agagtcgcaa aaaatattaa
    30181 tccatagtga ttat
//
LOCUS       DAZKHF020000027        30090 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2201S31241
            SAMN43267800-rid25106413.denovo.027, whole genome shotgun sequence.
ACCESSION   DAZKHF020000027 DAZKHF020000000
VERSION     DAZKHF020000027.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267800
            Sequence Read Archive: SRR30307263
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 30090)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 30090)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 30090)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350581.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350581.2
            Genome Coverage       :: 151x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:21:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,652
            CDSs (total)                      :: 5,557
            Genes (coding)                    :: 5,320
            CDSs (with protein)               :: 5,320
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 237
            CDSs (without protein)            :: 237
            Pseudo Genes (ambiguous residues) :: 0 of 237
            Pseudo Genes (frameshifted)       :: 102 of 237
            Pseudo Genes (incomplete)         :: 130 of 237
            Pseudo Genes (internal stop)      :: 52 of 237
            Pseudo Genes (multiple problems)  :: 41 of 237
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..30090
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267800-rid25106413.denovo.027"
                     /serotype="O 26:H11"
                     /isolate="2201S31241"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-01"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            111..1694
                     /gene="yegH"
                     /locus_tag="ACHOSC_RS05025"
                     /old_locus_tag="pgaptmp_001005"
     CDS             111..1694
                     /gene="yegH"
                     /locus_tag="ACHOSC_RS05025"
                     /old_locus_tag="pgaptmp_001005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TerC family protein"
                     /protein_id="HGU4546988.1"
                     /translation="MEWIADPSIWAGLITLIVIELVLGIDNLVFIAILAEKLPPKQRD
                     RARVTGLLLAMLMRLLLLASISWLVTLTQPLFSFCSFTFSARDLIMLFGGFFLLFKAT
                     MELNERLEGKDSNNPTQRKGAKFWGVVTQIVVLDAIFSLDSVITAVGMVDHLLVMMAA
                     VVIAISLMLMASKPLTQFVNSHPTIVILCLSFLLMIGFSLVAEGFGFVIPKGYLYAAI
                     GFSVMIEALNQLAIFNRRRFLSANQTLRQRTTEAVMRLLSGQKEDAELDAETASMLVD
                     HGNQQIFNPQERRMIERVLNLNQRTVSSIMTSRHDIEHIDLNAPEEEIRQLLERNQHT
                     RLVVTDGDDAEDLLGVVHVIDLLQQSLRGEPLNLRVLIRQPLVFPETLPLLPALEQFR
                     NARTHFAFVVDEFGSVEGIVTLSDVTETIAGNLPNEVEEIDARHGIQKNADGSWTANG
                     HMPLEDLVQYVPLPLDEKREYHTIAGLLMEYLQRIPKPGEEVQVGDYLLKTLQVESHR
                     VQKVQIIPLHKDGELEYEV"
     gene            complement(1968..3821)
                     /gene="asmA"
                     /locus_tag="ACHOSC_RS05030"
                     /old_locus_tag="pgaptmp_001006"
     CDS             complement(1968..3821)
                     /gene="asmA"
                     /locus_tag="ACHOSC_RS05030"
                     /old_locus_tag="pgaptmp_001006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane assembly protein AsmA"
                     /protein_id="HGU4546989.1"
                     /translation="MRRFLTTLMILLVVLVAGLSALVLLVNPNDFRDYMVKQVAARSG
                     YQLQLDGPLRWHVWPQLSILSGRMSLTAQGASQPLVRADNMRLDVALLPLLSHQLSVK
                     QVMLKGAVIQLTPQTEAVRSEDAPVAPRDNTLPDLSDDRGWSFDISSLKVADSVLVFQ
                     HEDDEQVTIRNIRLQMEQDPQHRGSFEFSGRVNRDQRDLTISLNGTVDASDYPHDLTA
                     AIEQINWQLQGADLPKQGIQGQGSFQAQWQESHKRLSFNQISLTANDSTLSGQAQVTL
                     TEKPEWQLRLQFPQLNLDNLIPLNETANGENGAAQQGQSQSTLPRPVISSRIDEPAYQ
                     GLQGFTADILLQASNVRWRGMNFTDVATQMTNKSGLLEITQLQGKLNGGQVSLPGTLD
                     ATSINPRINFQPRLENVEIGTILKAFNYPISLTGKMSLAGDFSGADIDADAFRHNWQG
                     QAHVEMTDTRMEGMNFQQMIQQAVERNGGDVKAAENFDNVTRLDRFTTDLTLKDGVVT
                     LNDMQGQSPVLALTGEGMLNLADQTCDTQFDIRVVGGWNGESKLIDFLKETPVPLRVY
                     GNWQQLNYSLQVDQLLRKHLQDEAKRRLNDWAERNKDSRNGKDVKKLLEKM"
     gene            complement(3843..4424)
                     /gene="dcd"
                     /locus_tag="ACHOSC_RS05035"
                     /old_locus_tag="pgaptmp_001007"
     CDS             complement(3843..4424)
                     /gene="dcd"
                     /locus_tag="ACHOSC_RS05035"
                     /old_locus_tag="pgaptmp_001007"
                     /EC_number="3.5.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004942378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008829 - dCTP deaminase activity
                     [Evidence IEA];
                     GO_process: GO:0006229 - dUTP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dCTP deaminase"
                     /protein_id="HGU4546990.1"
                     /translation="MRLCDRDIEAWLDEGRLSINPRPPVERINGATVDVRLGNKFRTF
                     RGHTAAFIDLSGPKDEVSAALDRVMSDEIVLDEGEAFYLHPGELALAVTLESVTLPAD
                     LVGWLDGRSSLARLGLMVHVTAHRIDPGWSGCIVLEFYNSGKLPLALRPGMLIGALSF
                     EPLSGPAARPYNRREDAKYRNQQGAVASRIDKD"
     gene            complement(4516..5157)
                     /gene="udk"
                     /locus_tag="ACHOSC_RS05040"
                     /old_locus_tag="pgaptmp_001008"
     CDS             complement(4516..5157)
                     /gene="udk"
                     /locus_tag="ACHOSC_RS05040"
                     /old_locus_tag="pgaptmp_001008"
                     /EC_number="2.7.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004849 - uridine kinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uridine kinase"
                     /protein_id="HGU4546991.1"
                     /translation="MTDQSHQCVIIGIAGASASGKSLIASTLYRELREQVGDEHIGVI
                     PEDCYYKDQSHLSMEERVKTNYDHPSAMDHSLLLEHLQALKRGSAIDLPVYSYVEHTR
                     MKETVTVEPKKVIILEGILLLTDARLRDELNFSIFVDTPLDICLMRRIKRDVNERGRS
                     MDSVMAQYQKTVRPMFLQFIEPSKQYADIIVPRGGKNRIAIDILKAKISQFFE"
     gene            5475..8785
                     /gene="dgcE"
                     /locus_tag="ACHOSC_RS05045"
                     /old_locus_tag="pgaptmp_001009"
                     /pseudo
     CDS             5475..8785
                     /gene="dgcE"
                     /locus_tag="ACHOSC_RS05045"
                     /old_locus_tag="pgaptmp_001009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416571.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
     gene            complement(8824..9681)
                     /gene="alkA"
                     /locus_tag="ACHOSC_RS05050"
                     /old_locus_tag="pgaptmp_001010"
     CDS             complement(8824..9681)
                     /gene="alkA"
                     /locus_tag="ACHOSC_RS05050"
                     /old_locus_tag="pgaptmp_001010"
                     /EC_number="3.2.2.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000288352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase 2"
                     /protein_id="HGU4546992.1"
                     /translation="MYTLNWQPPYDWSWMLGFLAARAVSGVETVAEDYYARSLAVGEY
                     RGVVTAIPDIARHTLHINLSAGLEPVAAECLAKMSRLFDLQCNPQIVNGALGKLGAAR
                     PGLRLPGCIDAFEQGVRAILGQLVSVAMAAKLTAKVVQLYGERLDDFPEYICFPTPQR
                     LAAADPQALKALGMPLKRAEALIHLANAALEGSLPMTIPGDVEQAMKTLQTFPGIGRW
                     TANYFALRGWQAKDVFLPDDYLIKQRFPGMTPAQIRRYAERWKPWRSYALLHIWYTEG
                     WQPDGTDEL"
     gene            9815..11167
                     /gene="yegD"
                     /locus_tag="ACHOSC_RS05055"
                     /old_locus_tag="pgaptmp_001011"
     CDS             9815..11167
                     /gene="yegD"
                     /locus_tag="ACHOSC_RS05055"
                     /old_locus_tag="pgaptmp_001011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416573.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone"
                     /protein_id="HGU4546993.1"
                     /translation="MFIGFDYGTANCSVAVMRDGKPQLLKMENDSTLLPSMLCAPTRE
                     SVSEWLYRHHDVPADDDETQALLRRAIRYNREEDIDVTAKSVQFGLSSLAQYIDDPEE
                     VWFVKSPKSFLGASGLKPQQVALFEDLVCAMMLHIRQQAQAQLPEAITQAVIGRPINF
                     QGLGGDEANAQAQGILERAAKRAGFRDVVFQYEPVAAGLDYEATLQEEKRVLVVDIGG
                     GTTDCSLLLMGPQWRSRLDREASLLGHSGCRIGGNDLDIALAFKNLMPLLGMGGETEK
                     GIALPILPWWNAVAINDVPAQSDFYSSANGRLLNDLVRDAREPEKVALLQKVWRERLS
                     YRLVRSAEECKIALSSVAETRASLPFISDELATLISQQGLESALSQPLARILEQVQLA
                     LDNAQEKPDVIYLTGGSARSPLIKKALAEQLPGIPIAGGDDFGSVTAGLARWAEVVFR
                     "
     gene            complement(11200..13119)
                     /gene="yegI"
                     /locus_tag="ACHOSC_RS05060"
                     /old_locus_tag="pgaptmp_001012"
     CDS             complement(11200..13119)
                     /gene="yegI"
                     /locus_tag="ACHOSC_RS05060"
                     /old_locus_tag="pgaptmp_001012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310906.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase YegI"
                     /protein_id="HGU4546994.1"
                     /translation="MKPTLYTATGECVTPGRELGKGGEGAVYDINEFVDSVAKIYHTP
                     PPALKQDKLAFMAATADAQLLNYVAWPQATLHGGRGGKVIGFMMPKVSGKEPIHMIYS
                     PAHRRQRYPHCAWDFLLYVARNIASSFATVHEHGHVVGDVNQNSFMVGRDSKVVLIDS
                     DSFQINANGTLHLCEVGVSHFTPPELQTLSSFVGFERTENHDNFGLALLIFHVLFGGR
                     HPYSGVPLISDAGNALETDITHFRYAYASDNQRRGLKPPPRSIPLSMLPGDVEAMFQQ
                     AFTESGVATGRPTAKAWVAALDSLRQQLKKCPVSAMHVYPAHLTDCPWCALDNQGVIY
                     FIDLGEEVITTGGDFVLAKVWAMVMASVAPPALQLPLPDHFQPTGRPLPLGLLRREYI
                     ILIEIALSALSLLLCGLQAEPRYIILVPVLSAIWIIGSLTSKAYKAEIQQRREAFNRA
                     KMDYEHLVSQIQQLGGLEGFIAKRAMLEKMKDEILGLPEEEKRALAALHDTARERQKQ
                     KFLEGFFIDVASIPGVGPARKAALRSFGIETAADVTRRGVKQVKGFGDHLTQAVIDWK
                     ASCERRFVFRPNEAITPADRQAVMAKMTAKRHRLESTLTVGATELQRFRLHAPARTMP
                     LMEPLRQAAEKLAQA"
     gene            complement(13116..13877)
                     /gene="pphC"
                     /locus_tag="ACHOSC_RS05065"
                     /old_locus_tag="pgaptmp_001013"
     CDS             complement(13116..13877)
                     /gene="pphC"
                     /locus_tag="ACHOSC_RS05065"
                     /old_locus_tag="pgaptmp_001013"
                     /EC_number="3.1.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004722 - protein serine/threonine
                     phosphatase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PP2C family serine/threonine-protein
                     phosphatase"
                     /protein_id="HGU4546995.1"
                     /translation="MSWRLVYASAVGTSHISADLPCQDACQMQVAWLNDQQPLLVMFL
                     ADGAGSVSQGGEGATLAVNEAMAYMSQKVQGGELGLNDILATDIVLTVRQRLFAEAEA
                     KELAVRDFACTFLGLISSANGTLIMQIGDGGVVVDFGHGLQLPLTPMVGEYANMTHFI
                     TDEDAVSRLETFTSTERVHKVAAFTDGIQRLALNMLDNSPHVPFFTPFFNGLASATQE
                     QLDLLPELLKQFLSSPAVNERTDDDKTLALALWLP"
     gene            complement(13874..14533)
                     /gene="yegL"
                     /locus_tag="ACHOSC_RS05070"
                     /old_locus_tag="pgaptmp_001014"
     CDS             complement(13874..14533)
                     /gene="yegL"
                     /locus_tag="ACHOSC_RS05070"
                     /old_locus_tag="pgaptmp_001014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HGU4546996.1"
                     /translation="MSEQITFATSDFASNPEPRCPCILLLDVSGSMSGRPINELNAGL
                     VTFRDELLADPLALKRVELGIVTFGPVHVEQPFTSAANFFPPILFAQGDTPMGAAITK
                     ALDMVEERKREYRANGISYYRPWIFLITDGAPTDEWQAAANKVFRGEEDKRFAFFSIG
                     VQGADMKTLAQISVRQPLPLQGLQFRELFSWLSSSLRSVSRSTPGTEVVLEAPKGWTS
                     V"
     gene            complement(14754..14813)
                     /gene="ibsA"
                     /locus_tag="ACHOSC_RS05075"
                     /old_locus_tag="pgaptmp_001015"
     CDS             complement(14754..14813)
                     /gene="ibsA"
                     /locus_tag="ACHOSC_RS05075"
                     /old_locus_tag="pgaptmp_001015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin IbsA"
                     /protein_id="HGU4546997.1"
                     /translation="MMKHVIILVILLVISFQAY"
     gene            complement(15086..15142)
                     /locus_tag="ACHOSC_RS05080"
                     /old_locus_tag="pgaptmp_001016"
     CDS             complement(15086..15142)
                     /locus_tag="ACHOSC_RS05080"
                     /old_locus_tag="pgaptmp_001016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Ibs family toxin"
                     /protein_id="HGU4546998.1"
                     /translation="MMKLLIIVVLLVISFPAY"
     gene            complement(15414..15470)
                     /locus_tag="ACHOSC_RS05085"
                     /old_locus_tag="pgaptmp_001017"
     CDS             complement(15414..15470)
                     /locus_tag="ACHOSC_RS05085"
                     /old_locus_tag="pgaptmp_001017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Ibs family toxin"
                     /protein_id="HGU4546999.1"
                     /translation="MMKLLIIVVLLVISYPAY"
     gene            15749..16996
                     /gene="mdtA"
                     /locus_tag="ACHOSC_RS05090"
                     /old_locus_tag="pgaptmp_001018"
     CDS             15749..16996
                     /gene="mdtA"
                     /locus_tag="ACHOSC_RS05090"
                     /old_locus_tag="pgaptmp_001018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416578.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter subunit MdtA"
                     /protein_id="HGU4547000.1"
                     /translation="MKGSYKSRWVIVIVVVIAAIAAFWFWQGRNDSRSAAPGATKQAQ
                     QSPAGGRRGMRSGPLAPVQAATAVEQAVPRYLTGLGTIIAANTVTVRSRVDGQLMALH
                     FQEGQQVKAGDLLAEIDPSQFKVALAQTQGQLAKDKATLANARRDLARYQQLAKTNLV
                     SRQELDAQQALVSETEGTIKADEASVASAQLQLDWSRITAPVDGRVGLKQVDVGNQIS
                     SGDTTGIVVITQTHPIDLVFTLPESDIATVVQAQKAGKPLVVEAWDRTNSKKLSEGTL
                     LSLDNQIDATTGTIKVKARFNNQDDALFPNQFVNARMLVDTEQNAVVIPTAALQMGNE
                     GHFVWVLNSENKVSKHLVTPGIQDSQKVVIRAGISAGDRVVTDGIDRLTEGAKVEVVE
                     AQSATTPEEKATSREYAKKGARS"
     gene            16996..20118
                     /gene="mdtB"
                     /locus_tag="ACHOSC_RS05095"
                     /old_locus_tag="pgaptmp_001019"
     CDS             16996..20118
                     /gene="mdtB"
                     /locus_tag="ACHOSC_RS05095"
                     /old_locus_tag="pgaptmp_001019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter permease
                     subunit MdtB"
                     /protein_id="HGU4547001.1"
                     /translation="MQVLPPSSTGGPSRLFIMRPVSTTLLMVAILLAGIIGYRALPVS
                     ALPEVDYPTIQVVTLYPGASPDVMTSAVTAPLERQFGQMSGLKQMSSQSSGGASVITL
                     QFQLTLPLDVAEQEVQAAINAATNLLPSDLPNPPVYSKVNPADPPIMTLAVTSTAMPM
                     TQVEDMVETRVAQKISQISGVGLVTLSGGQRPAVRVKLNAQAIAALGLTSETVRTAIT
                     GANVNSAKGSLDGPSRAVTLSANDQMQSAEEYRQLIIAYQNGAPIRLGDVATVEQGAE
                     NSWLGAWANKEQAIVMNVQRQPGANIISTADSIRQMLPQLTESLPKSVKVTVLSDRTT
                     NIRASVDDTQFELMMAIALVVMIIYLFLRNIPATIIPGVAVPLSLIGTFAVMVFLDFS
                     INNLTLMALTIATGFVVDDAIVVIENISRYIEKGEKPLAAALKGAGEIGFTIISLTFS
                     LIAVLIPLLFMGDIVGRLFREFAITLAVAILISAVVSLTLTPMMCARMLSQESLRKQN
                     RFSRASEKMFDRIIAAYGRGLAKVLNHPWLTLSVALSTLLLSVLLWVFIPKGFFPVQD
                     NGIIQGTLQAPQSSSFANMAQRQRQVADVILQDPAVQSLTSFVGVDGTNPSLNSARLQ
                     INLKPLDERDDRVQKVIARLQTAVDKVPGVDLFLQPTQDLTIDTQVSRTQYQFTLQAT
                     SLDALSTWVPQLMEKLQQLPQLSDVSSDWQDKGLVAYVNVDRDSASRLGISMADVDNA
                     LYNAFGQRLISTIYTQANQYRVVLEHNTEITPGLAALDTIRLTSSDGGVVPLSSIAKV
                     EQRFAPLSINHLDQFPVTTISFNVPDNYSLGDAVQAIMDTEKTLNLPVDITTQFQGST
                     LAFQSALGSTVWLIVAAVVAMYIVLGILYESFIHPLTILSTLPTAGVGALLALMIAGS
                     ELDVIAIIGIILLIGIVKKNAIMMIDFALAAEREQGMSPRDAIYQACLLRFRPILMTT
                     LAALLGALPLMLSTGVGAELRRPLGIGMVGGLIVSQVLTLFTTPVIYLLFDRLALWTK
                     SRFARHEEEA"
     gene            20119..23196
                     /gene="mdtC"
                     /locus_tag="ACHOSC_RS05100"
                     /old_locus_tag="pgaptmp_001020"
     CDS             20119..23196
                     /gene="mdtC"
                     /locus_tag="ACHOSC_RS05100"
                     /old_locus_tag="pgaptmp_001020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter permease
                     subunit MdtC"
                     /protein_id="HGU4547002.1"
                     /translation="MKFFALFIYRPVATILLSVAITLCGILGFRMLPVAPLPQVDFPV
                     IMVSASLPGASPETMASSVATPLERSLGRIAGVSEMTSSSSLGSTRIILQFDFDRDIN
                     GAARDVQAAINAAQSLLPSGMPSRPTYRKANPSDAPIMILTLTSDTYSQGELYDFAST
                     QLAPTISQIDGVGDVDVGGSSLPAVRVGLNPQALFNQGVSLDDVRTAISNANVRKPQG
                     ALEDGTHRWQIQTNDELKTAAEYQPLIIHYNNGGAVRLGDVATVTDSVQDVRNAGMTN
                     AKPAILLMIRKLPEANIIQTVDSIRAKLPELQETIPAAIDLQIAQDRSPTIRASLEEV
                     EQTLIISVALVILVVFLFLRSGRATIIPAVAVPVSLIGTFAAMYLCGFSLNNLSLMAL
                     TIATGFVVDDAIVVLENIARHLEAGMKPLQAALQGTREVGFTVLSMSLSLVAVFLPLL
                     LMGGLPGRLLREFAVTLSVAIVISLLVSLTLTPMMCGWMLKASKPREQKRLRGFGRML
                     VALQQGYGKSLKWVLNHTRLVGVVLLGTIALNIWLYISIPKTFFPEQDTGVLMGGIQA
                     DQSISFQAMRGKLQDFMKIIRDDPAVDNVTGFTGGSRVNSGMMFITLKPRDERSETAQ
                     QIIDRLRVKLAKEPGANLFLMAVQDIRVGGRQSNASYQYTLLSDDLAALREWEPKIRK
                     KLATLPELADVNSDQQDNGAEMNLVYDRDTMARLGIDVQAANSLLNNAFGQRQISTIY
                     QPMNQYKVVMEVDPRYTQDISALEKMFVINNEGKAIPLSYFAKWQPANAPLSVNHQGL
                     SAASTISFNLPTGKSLSDASAAIDRAMTQLGVPSTVRGSFAGTAQVFQETMNSQVILI
                     IAAIATVYIVLGILYESYVHPLTILSTLPSAGVGALLALELFNAPFSLIALIGIMLLI
                     GIVKKNAIMMVDFALEAQRHGNLTPQEAIFQACLLRFRPIMMTTLAALFGALPLVLSG
                     GDGSELRQPLGITIVGGLVMSQLLTLYTTPVVYLFFDRLRLRFSRKPKQTVTE"
     gene            23197..24612
                     /gene="mdtD"
                     /locus_tag="ACHOSC_RS05105"
                     /old_locus_tag="pgaptmp_001021"
     CDS             23197..24612
                     /gene="mdtD"
                     /locus_tag="ACHOSC_RS05105"
                     /old_locus_tag="pgaptmp_001021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HGU4547003.1"
                     /translation="MTDLPDSTRWQLWIVAFGFFMQSLDTTIVNTALPSMAQSLGESP
                     LHMHMVIVSYVLTVAVMLPASGWLADKVGVRNIFFTAIVLFTLGSLFCALSGTLNELL
                     LARALQGVGGAMMVPVGRLTVMKIVPREQYMAAMTFVTLPGQVGPLLGPALGGLLVEY
                     ASWHWIFLINIPVGIIGAIATLMLMPNYTMQTRRFDLSGFLLLAVGMAVLTLALDGSK
                     GTGLSPLAIAGLVAVGVVALVLYLLHARNNNRALFSLKLFRTRTFSLGLAGSFAGRIG
                     SGMLPFMTPVFLQIGLGFSPFHAGLMMIPMVLGSMGMKRIVVQVVNRFGYRRVLVATT
                     LGLSLVTLLFMTTALLGWYYVLPFVLFLQGMVNSTRFSSMNTLTLKDLPDNLASSGNS
                     LLSMIMQLSMSIGVTIAGLLLGLFGSQHVSVDSGTTQTVFMYTWLSMALIIALPAFIF
                     ARVPNDTHQNVAISRRKRSAQ"
     gene            24609..26012
                     /gene="baeS"
                     /locus_tag="ACHOSC_RS05110"
                     /old_locus_tag="pgaptmp_001022"
     CDS             24609..26012
                     /gene="baeS"
                     /locus_tag="ACHOSC_RS05110"
                     /old_locus_tag="pgaptmp_001022"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     BaeS"
                     /protein_id="HGU4547004.1"
                     /translation="MKFWRPGITGKLFLAIFATCIVLLISMHWAVRISFERGFIDYIK
                     HGNEQRLQLLSDALGEQYAQHGNWRFLRNNDRFVFQILRSFEHDNSEDKPGPGMPPHG
                     WRTQFWVVDQNNKVLVGPRAPIPPDGTRRPILVNGAEVGAVIASPVERLTRNTDINFD
                     KQQRQTSWLIVALATLLAALATFLLARGLLAPVKRLVDGTHKLAAGDFTTRVTPTSED
                     ELGKLAQDFNQLASTLEKNQQMRRDFMADISHELRTPLAVLRGELEAIQDGVRKFTPE
                     TVASLQAEVGTLTKLVDDLHQLSMSDEGALAYQKAPVDLIPLLEVAGGAFRERFASRG
                     LKLQFSLPDSITVFGDRDRLMQLFNNLLENSLRYTDSGGSLKISAEQHDKTVRLTFAD
                     SAPGVSDEQLQKLFERFYRTEGSRNRASGGSGLGLAICLNIVEAHNGRIIAAHSPFGG
                     VSITVELPLERDLQREV"
     gene            26009..26731
                     /gene="baeR"
                     /locus_tag="ACHOSC_RS05115"
                     /old_locus_tag="pgaptmp_001023"
     CDS             26009..26731
                     /gene="baeR"
                     /locus_tag="ACHOSC_RS05115"
                     /old_locus_tag="pgaptmp_001023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator BaeR"
                     /protein_id="HGU4547005.1"
                     /translation="MTELPIDENTPRILIVEDEPKLGQLLIDYLRAASYAPTLISHGD
                     QVLPYVRQTPPDLILLDLMLPGTDGLTLCREIRRFSDIPIVMVTAKIEEIDRLLGLEI
                     GADDYICKPYSPREVVARVKTILRRCKPQRELQQQDAESPLIIDEGRFQASWRGKMLD
                     LTPAEFRLLKTLSHEPGKVFSREHLLNHLYDDYRVVTDRTIDSHIKNLRRKLESLDAE
                     QSFIRAVYGVGYRWEADACRIV"
     gene            26922..27254
                     /gene="yegP"
                     /locus_tag="ACHOSC_RS05120"
                     /old_locus_tag="pgaptmp_001024"
     CDS             26922..27254
                     /gene="yegP"
                     /locus_tag="ACHOSC_RS05120"
                     /old_locus_tag="pgaptmp_001024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310915.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YegP family protein"
                     /protein_id="HGU4547006.1"
                     /translation="MAGWFELSKSSDNQFRFVLKAGNGETILTSELYTSKASAEKGIA
                     SVRSNSPQEERYEKKTASNGKFYFNLKAANHQIIGSSQMYATAQSRETGIASVKANGT
                     SQTVKDNT"
     gene            27463..27759
                     /locus_tag="ACHOSC_RS05125"
                     /old_locus_tag="pgaptmp_001025"
     CDS             27463..27759
                     /locus_tag="ACHOSC_RS05125"
                     /old_locus_tag="pgaptmp_001025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001307279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0051983 - regulation of chromosome
                     segregation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system ParD family
                     antitoxin"
                     /protein_id="HGU4547007.1"
                     /translation="MTYTDVRFLLTGGRKMARTMTVDLGDELREFIESLIESGDYRTQ
                     SEVIRESLRLLREKQAESRLQALRDMLAEGLSSGEAQPWEKDAFLRKVKAGIRK"
     gene            27761..28057
                     /locus_tag="ACHOSC_RS05130"
                     /old_locus_tag="pgaptmp_001026"
     CDS             27761..28057
                     /locus_tag="ACHOSC_RS05130"
                     /old_locus_tag="pgaptmp_001026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001220181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008657 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) inhibitor activity
                     [Evidence IEA];
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA];
                     GO_process: GO:0030541 - plasmid partitioning [Evidence
                     IEA];
                     GO_process: GO:0051290 - protein heterotetramerization
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HGU4547008.1"
                     /translation="MRIIKLMPKANEDLEGIWYYSYHHFGEPQADRYVEHLSDVLQIL
                     SNNNIGTPRPELGEGIFVLPFERHVIYFLQSPGEIIVIRILNQNQDATRHLHWS"
     gene            28160..29521
                     /gene="yegQ"
                     /locus_tag="ACHOSC_RS05135"
                     /old_locus_tag="pgaptmp_001027"
     CDS             28160..29521
                     /gene="yegQ"
                     /locus_tag="ACHOSC_RS05135"
                     /old_locus_tag="pgaptmp_001027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 5-hydroxyuridine modification protein YegQ"
                     /protein_id="HGU4547009.1"
                     /translation="MFKPELLSPAGTLKNMRYAFAYGADAVYAGQPRYSLRVRNNEFN
                     HENLQLGINEAHALGKKFYVVVNIAPHNAKLKTFIRDLKPVVEMGPDALIMSDPGLIM
                     LVREHFPEMPIHLSVQANAVNWATVKFWQQMGLTRVILSRELSLEEIEEIRNQVPDME
                     IEIFVHGALCMAYSGRCLLSGYINKRDPNQGTCTNACRWEYNVQEGKEDDVGNIVHKY
                     EPIPVQNVEPTLGIGAPTDKVFMIEEAQRPGEYMTAFEDEHGTYIMNSKDLRAIAHVE
                     RLTKMGVHSLKIEGRTKSFYYCARTAQVYRKAIDDAAAGKPFDTSLLETLEGLAHRGY
                     TEGFLRRHTHDDYQNYEYGYSVSDRQQFVGEFTGERKGDLAAVAVKNKFSVGDSLELM
                     TPQGNINFTLEHMENAKGEAMPIAPGDGYTVWLPVPQDLELNYALLMRNFSGETTRNP
                     HGK"
     gene            complement(29851..>30090)
                     /gene="yegR"
                     /locus_tag="ACHOSC_RS05140"
                     /old_locus_tag="pgaptmp_001028"
     CDS             complement(29851..>30090)
                     /gene="yegR"
                     /locus_tag="ACHOSC_RS05140"
                     /old_locus_tag="pgaptmp_001028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310918.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YegR"
                     /protein_id="HGU4547010.1"
                     /translation="IGKFGLAYKSNIQRKLDNQYYTEAEASLARGRISGAENIVKNDA
                     AHFCVTQGKKMQIVDLKTEGAGLHGVARLTFKCGE"
BASE COUNT         6941 a         8030 c         7961 g         7158 t
ORIGIN      
        1 accctcaaat aatatgagat aaatagtgct gcaacattgc atttttgccc cgatttatcc
       61 atgatcgaat tatgacattt gtcatacaac gaataggttt tgtacttact atggaatgga
      121 ttgccgatcc gtctatctgg gccgggttaa tcacgctgat tgtgatcgaa ctggtcctcg
      181 gcattgataa cctggtcttt attgccatcc tcgccgaaaa actaccgccg aagcagcgcg
      241 accgcgcacg ggttaccggg ctgctgctgg cgatgttaat gcgcctgtta ctgctggcgt
      301 caatctcctg gctggtcacc ctgactcaac cgctgttcag cttctgctcg tttaccttta
      361 gcgcccgcga cttaatcatg ctgtttggtg gtttcttcct gttgttcaaa gccacgatgg
      421 agctgaacga acggctggaa gggaaagaca gcaataatcc cacacaacgc aaaggtgcga
      481 agttctgggg cgtggtgacg caaattgtgg tactggacgc catcttctca ctcgactcag
      541 tgattaccgc cgtcgggatg gtcgaccatt tactggtcat gatggccgcc gtggttatcg
      601 ctatcagcct gatgttgatg gccagcaagc cgttaacgca atttgttaac agtcacccga
      661 ccatcgttat tctctgctta agcttcctgt tgatgattgg ctttagcctg gtggcagaag
      721 gtttcggctt cgtcattccg aaaggctacc tgtacgctgc cattggtttc tcggtgatga
      781 tcgaggcgct caatcagttg gctatcttta accggcgacg ttttctttcc gctaaccaga
      841 cgctgcgcca gcggactacc gaagcggtaa tgcgcctgct tagcgggcaa aaagaagatg
      901 cggaactgga cgccgaaacc gcgtccatgc tggtggatca tggtaaccag cagatcttta
      961 atccgcagga acggcggatg attgagcggg tacttaatct taaccagcgt actgtcagca
     1021 gcattatgac gtcgcgccac gatattgagc atatcgatct caacgcgccg gaagaagaaa
     1081 tccgccagtt gctggagcga aatcagcata cgcggctggt ggttaccgac ggtgatgacg
     1141 cagaagattt gctcggtgtt gttcacgtta tcgacctgct acaacagtca ctgcgcggcg
     1201 aaccgctcaa cctgcgggtg ttgattcgcc agccgctggt gttcccggaa accttgccgt
     1261 tgttacctgc cctggagcag ttccgtaatg cccgcacgca ctttgctttt gtagtggatg
     1321 agtttggctc ggtggaaggg attgtgacat taagtgacgt cactgaaacc attgccggta
     1381 acttaccgaa cgaagtggaa gagatcgacg cccgccatgg tattcagaag aatgcggacg
     1441 gttcctggac ggcgaatggt catatgccgc tggaagatct ggtgcaatat gtgccgctgc
     1501 cgctggatga aaaacgtgaa tatcacacca ttgccgggct gttgatggaa tatttacagc
     1561 gtattccaaa acctggcgaa gaagttcagg tgggggatta tttgcttaaa acgttgcagg
     1621 tggaaagcca tcgcgtgcag aaggtgcaga ttattccgct gcataaggat ggcgagttgg
     1681 agtacgaggt gtgatggtat cggtcatttt acccgatagc cgaaaggaat aagatgaggg
     1741 aaccgcgcca gcggcgattt accgccggga gcccgggttg ccagggtggt ggcgattgag
     1801 ccaccctggc acgttcaccg gcttgagcgt tccatagtag tgaggaacat caagtgaacg
     1861 gaacagcctt cgatcttaca tattcagaat ataatggtgc cgccgcgaac gcctgatgcg
     1921 acgctttacg cgtcttatca ggcctacagg acaggtcacc tggtaaccta catcttctcc
     1981 agcaacttct tcacatcttt gccattgcgg gaatctttat tccgctcggc ccagtcattc
     2041 aggcgacgtt tcgcttcgtc ctgtagatgt ttgcgcagta actgatccac ttgcagactg
     2101 taattgagtt gctgccagtt gccataaacc cgcagcggta ctggcgtttc tttgaggaaa
     2161 tcaatcagtt tgctttcccc gttccagcca cccacgaccc gaatatcaaa ctgggtgtcg
     2221 caggtttgat ctgccagatt caacatgcct tcccctgtca gcgccagcac aggcgattga
     2281 ccttgcatgt cgtttaacgt cacgacgcca tccttcaacg tcaaatcggt ggtaaagcgg
     2341 tcaagacgcg ttacgttatc gaagttttca gcggccttca catcaccacc attacgttct
     2401 accgcttgct gaatcatctg ctggaagttc atcccttcca tgcgcgtgtc ggtcatttcg
     2461 acatgtgcct gtccttgcca gttgtggcgg aatgcgtcgg catctatgtc agcaccggag
     2521 aaatcaccag ccagtgacat ttttccggtc aacgaaatcg gatagttaaa cgccttcaga
     2581 atggtaccaa tctcaacgtt ttccagccgt ggctggaagt ttatccgcgg atttattgat
     2641 gtcgcgtcca gcgtgcccgg cagtgaaacc tgtccaccgt taagcttgcc ctgcagttga
     2701 gtaatttcca gcaaacccga cttgttggtc atttgcgtgg caacatctgt aaaattcatt
     2761 ccgcgccagc gcacgttact ggcctgcaac aaaatatcag ccgtaaagcc ttgcagtccc
     2821 tgatatgccg gttcatcaat acgcgaagaa atgaccgggc gcggcaatgt tgattggctc
     2881 tgcccctgct gcgcggcacc gttttcacca ttcgctgttt cattaagcgg gatgaggttg
     2941 tcaagattca gttgcgggaa ttgcagtctc agctgccatt caggtttctc tgtcagcgtg
     3001 acctgtgctt gcccgctcag cgtgctgtca ttggcggtca agctgatttg gttaaatgaa
     3061 aggcgtttat gtgactcctg ccactgggcc tggaaactcc cctgcccctg aataccttgt
     3121 tttggtaaat ctgcaccctg caactgccag ttaatttgtt caatagccgc cgttaaatca
     3181 tgcggataat cagaagcatc taccgtaccg ttaagggata tcgtgagatc gcgctgatcg
     3241 cgattaaccc gcccggagaa ctcaaatgag ccacgatgtt ggggatcttg ttccatttgc
     3301 aggcggatat tgcggattgt cacctgctcg tcatcttcat gctggaacac cagcacgctg
     3361 tccgccacct taagactgga tatatcaaac gaccatccgc gatcgtctga cagatccggc
     3421 aaggtattgt cgcgcggtgc aaccggagcg tcttcactgc gcaccgcttc cgtctgcggc
     3481 gtcagttgga tcactgcccc ttttagcatc acctgcttaa cgctcagttg atgactcagt
     3541 agtggtaaaa gcgccacgtc cagacgcatg ttgtcggcgc gaaccagtgg ctggcttgcg
     3601 ccctgggcgg tgagagacat tcgcccggag aggatactaa gctgcggcca gacgtgccaa
     3661 cgcagtggcc cgtcgagctg caattgataa ccgctacgtg cagcaacttg cttgaccata
     3721 tagtcgcgga aatcattcgg attcaccagc aacactaacg cagataaccc ggccaccagc
     3781 acgaccagga gtatcatcag cgtcgtcaga aatcgtctca tggtatcctc aatgggcctg
     3841 aattagtctt tatcgattcg gctggctacc gcgccctgct ggttgcgata tttcgcatct
     3901 tcacggcggt tgtaaggtcg cgccgccggg ccggaaagcg gctcaaagct cagcgcacca
     3961 attaacatgc ccggacgcag cgccagcggc agcttaccgg agttgtagaa ctccagcaca
     4021 atgcaaccag accagcccgg atcgatacgg tgcgcggtga cgtgcaccat cagccccaga
     4081 cgcgccaaag aggaacgccc gtccagccag cccaccagat cggctggcag cgtcaccgac
     4141 tccagcgtca ccgccagcgc cagctctcct gggtgaagat agaacgcctc gccctcgtcg
     4201 agaacgatct catcgctcat cacgcggtca agcgcggcgc tcacttcatc tttgggaccg
     4261 ctcagatcga taaacgctgc cgtgtgacca cggaaggtac gaaatttatt gcccaggcgt
     4321 acatccaccg tcgcgccgtt aatacgctcc actggcggac gtgggttgat cgacaaacgg
     4381 ccttcatcaa gccaggcttc aatatctcgg tcacacagac gcatggcatt ttctcctttc
     4441 gcgcatcact cccttaacgc caattacgtc aagggcatac caggttatca ctgaacggta
     4501 cacaatttat caagcttatt caaagaactg acttattttc gctttcaata tatcgatcgc
     4561 gatgcggttt ttcccgccgc gcggcacgat aatgtccgca tattgtttag aaggctcaat
     4621 gaattgcagg aacatcgggc gcacggtttt ttgatattgc gccatcacgg aatccattga
     4681 acgcccacgc tcgttaacgt cacgcttgat gcggcgcatc aggcagatat ccagcggggt
     4741 atcaacgaaa atggagaagt taagttcgtc acgcaaacgc gcatccgtca gcaacaaaat
     4801 gccttcgaga atgatgacct tcttcggctc aaccgtcacc gtttctttca tacgcgtatg
     4861 ttcaacatag ctgtaaaccg gcaggtcaat tgccgagccg cgtttcaacg cttgtaaatg
     4921 ctcaagcaac aggctgtgat ccatcgcgct gggatggtcg tagttggtct taacgcgttc
     4981 ttccatcgac agatggcttt gatctttgta atagcagtct tcgggaatta cgccgatgtg
     5041 ttcatcaccg acttgctcac gtaattcacg ataaagggta ctggcaataa gactcttgcc
     5101 ggaagccgat gcgccagcga taccgataat gacgcactga tgagactgat cagtcatata
     5161 tttagcgacc tgattaacct ggatgttagg aagggggcga cgaagcgcca aacgcggcaa
     5221 ttatagggat ttcatccgcc tgataccagt cgaatagcgc tgccgcgcgc tcagagttaa
     5281 ttgttgacga agaattcccg gtggcaaatt acgttgatca gttttataca aggtaaaaaa
     5341 tgttatacgc agttgcgcaa attatccgcc tttacgtcac tttatgagca attcgcatat
     5401 aaaatgtaaa acttttgtac tagcataaac acagaaacga atactggcga cctggtcttg
     5461 cggataaagc ggtaatgagc aaacaatcac agcatgtatt aattgccctg ccccacccgc
     5521 tgcttcacct ggtcagttta ggtttagtct cgtttatctt tacccttttc tcgcttgagc
     5581 tttcgcagtt tggcacccaa ctcgccccac tgtggttccc gacgtccatc atgatggtgg
     5641 cgttttatcg ccatgccggg cgcatgtggc cgggaattgc gctgagctgc tcgctgggaa
     5701 atatcgccgc atccatcctg cttttttcca ccagctcgct gaacatgacc tggacgacca
     5761 tcaatattgt tgaagccgtg gtcggggcag tgctgctacg taaattgctg ccgtggtata
     5821 accccttgca aaatctggct gactggctgc gtctggcatt cggcagcgcc attgttccgc
     5881 ctctgctagg gggtgttctg gttgtcctgc tgacgcccgg agacgatcct ctcagggcat
     5941 ttttgatatg ggtactgtca gaatccatcg gcgctctggc actggtgccg ctgggattgt
     6001 tatttaaacc acactatctg ctgcgccatc gcaacccacg gttgcttttt gagtcgctgc
     6061 tcacgttagc catcacactg acgttaagct ggctttcgat gctgtatctg ccgtggcctt
     6121 ttactttcat tattgtgctg ttgatgtgga gcgccgtgcg cctgccacga atggaagcct
     6181 ttttgatctt ccttaccacg gtgatgatgg tgtcgctgat gatggccgcg gatccctccc
     6241 tgcttgctac gccgcgtaca tacctgatga gccatatgcc gtggctaccg tttttgctga
     6301 tcctgctgcc cgccaacatc atgacgatgg tgatgtatgc ctttcgtgcg gaacgcaaac
     6361 acatttccga aagcgaaacc cgttttcgga acgccatgga atattccgct atcggtatgg
     6421 cgttagtggg caccgaggga caatggctgc aaaccaacaa agcgctctgc cagtttctcg
     6481 ggtacagtca ggaagagctg cgcggactca cctttcagca actgacctgg ccggaggatc
     6541 tcaataaaga tctccaacag gttgaaaagc tgataagcgg tgaaataaac acctattcaa
     6601 tggaaaaacg ctactacaac cgcaatggcg atgttgtctg ggcgttgctt gccgtctcac
     6661 tggtgcgcca cacggatggc acgccgctct attttatcgc tcagattgaa gacattaacg
     6721 agctaaaacg caccgaacag gtgaatcagc aactgatgga gcgcatcacg ctggctaacg
     6781 aagcgggcgg gattggcatc tgggagtggg agttgaagcc gaatattttt agctgggata
     6841 agcggatgtt cgagctgtat gaaattcctc cacatatcaa accgaactgg caggtgtggt
     6901 acgagtgcgt gctgccggaa gatcgccagc atgccgaaaa agtgattcgt tgcaatcacg
     6961 ctcgcccttt aaactggaat ttcgcattac cgtaaaagac ggtattcgcc atatccgcgc
     7021 cctcgccaac cgggtactga ataaagaagg cgaagtcgaa cgcctgctcg gcattaatat
     7081 ggatatgact gaggttaaac agcttaacga ggcattgttt caggaaaaag agcgcctgca
     7141 cataacgctg gactccatcg gcgaagccgt ggtctgtatt gatatggcaa tgaaaattac
     7201 ctttatgaat ccagtggcgg agaagatgag cggctggacg caggaagaag cattaggtgt
     7261 tccgctcctg acggtgttgc atattacctt tggcgacaat ggtcccctga tggagaacat
     7321 ttacagtgcc gacacctcac gttccgcgat tgaacaagat gtggtgttgc actgccggag
     7381 cggcggcagc tacgacgtgc attacagtat tacgccgtta agtactctgg acggcagcaa
     7441 tattggttcg gttctggtga ttcaggacgt taccgaatca cgcaaaatgc tgcgccagct
     7501 gagctacagc gcctcccatg atgcactgac gcatctcgcc aatcgcgcca gttttgagaa
     7561 gcaactgcgt atgctgctgc aaacggtaaa cagtacacat cagcgacatg ccctggtgtt
     7621 tatcgatctt gatcgcttta aagcggtgaa tgacagcgcc gggcatgcgg cgggcgacgc
     7681 tttgctgcgc gaactggcgt cgttgatgct gagtatgctg cgttccagcg acgtgctggc
     7741 gcgactcggt ggggatgaat ttggtctgct gctaccagac tgtaatgttg aaagcgcgcg
     7801 ttttatcgct acacgcatta tcagtgccgt gaatgactat cactttatat gggaaggccg
     7861 tgtgcatcgg gtaggtgcca gtgccgggat taccttgatt gatgacaaca atcatcaggc
     7921 ggcagaagtg atgtcgcagg ctgatatcgc ctgttatgcc tccaaaaatg gtggacgggg
     7981 ccgggtgacg gtttacgaac cacagcaagc tgccgcacat agcgaacggg cggcgatgtc
     8041 gcttgatgaa cagtggcgga tgattaaaga gaatcagttg atgatgatgg cccacggtgt
     8101 cgcttcgccg cggatcccgg aagcgcgtaa tttgtggctg atttcgctta aactctggag
     8161 ttgcgaaggc gagattattg atgaacaaaa atttcgtcgt agcttcagcg atccggcact
     8221 tagccatgct cttgaccgac gggtattcca cgaatttttc cagctggccg caaaagcggt
     8281 tgccagtaaa ggcataagca tcgccctccc cctttccgtt gccggtttga gtagcgccac
     8341 gctggtgaat gatctgcttg agcagctgga aaatagccct ctaccaccac ggttattaca
     8401 tctgattatt ccggcagacg cgattttaga tcacgcagaa agcgtgcaaa aactgcggct
     8461 ggcgggatgt cggatagtgc tcagccaggt gggccgcgat ctgcaaatct tcaactcgct
     8521 gaaagcgaat atggcagatt acctgctact tgatggtgag ttatgcgcca acgtgcaggg
     8581 taatttgatg gatgagatgc tgattacgat tattcagggg cacgctcagc gactcgggat
     8641 gaaaaccatc gccgggccag tcgttttacc cttagtgatg gatacgcttt ctggcatcgg
     8701 cgtcgatctg atttatggtg atgtgattgc cgatgcccaa ccgctggatt tgctggtgaa
     8761 tagcagttat ttcgcgatta actgagggag cgtttgccca ttgcctgatg cgacgcatca
     8821 ggcctacaat tcgtccgttc cgtctggttg ccagccttcc gtataccaga tatgcaacag
     8881 cgcataagaa cgccagggct tccagcgctc ggcataacgg cggatttgtg ccggtgtcat
     8941 ccccggaaat cgctgtttaa tcagataatc atccggcaga aaaacatctt tcgcctgcca
     9001 gccacgcaaa gcgaaataat tcgccgtcca gcgcccgata cccggaaaag tttgcagcgt
     9061 tttcatcgcc tgctccacat cgcccggaat tgtcattggt aagctgccct ccagcgccgc
     9121 atttgccaga tgaatcagtg cctctgcccg tttcaacggc atgcctaacg cttttaatgc
     9181 ctgcgggtcg gctgctgcca gccgctgagg cgtggggaag cagatatact ccggaaaatc
     9241 atccagccgt tcgccataaa gctgtacaac tttggcagtc aattttgccg ccatcgccac
     9301 gctcaccagt tggcctaaaa tcgcccgcac accctgctca aaagcatcaa tacagccggg
     9361 taaacgcaat ccgggccgcg ccgcgcctaa cttgcccaac gcaccgttaa caatctgcgg
     9421 gttacattgc agatcaaaca ggcggctcat tttcgccaga cactcagcgg caacgggttc
     9481 taaacctgca cttaaattta tgtgcagagt atggcgggct atatccggaa tagcagtcac
     9541 cacgccgcga tattcgccca ccgccagact acgggcataa taatcctcag cgaccgtttc
     9601 cacaccactc actgcacggg cggcgagaaa tcccaacatc cacgaccagt catacggcgg
     9661 ctgccagttc agggtataca tcgcatctcc ttattcatcc gctttcagca taaacgttat
     9721 tcagacgcta cgctttgctt tcatattccg gttgtcgcga cagcaacatt tcgctaaagt
     9781 cacgcccctt cttcaccggc atggggatta attcgtgttt attggttttg attacggtac
     9841 agcaaactgt tcagtggcgg tcatgcgtga cggtaaaccg caattactga aaatggaaaa
     9901 cgacagcacg ctgctgcctt caatgctttg cgcgccaacg cgtgaatcgg taagcgaatg
     9961 gctgtaccgc catcatgatg ttccggcaga cgacgatgaa acgcaggcgc tgttacgtcg
    10021 ggcgattcgt tataaccgcg aagaagatat cgatgttacg gcgaaaagcg tgcagttcgg
    10081 tctttcctca ctggcacagt acattgatga tccagaagaa gtgtggtttg tgaaatcacc
    10141 aaaatcgttc ctcggtgcca gcggcttaaa accgcagcag gtagcgctgt ttgaggatct
    10201 ggtctgcgca atgatgttgc acattcgcca gcaggcgcag gcgcagctgc cagaagcgat
    10261 tactcaggcg gtgattggtc gcccgatcaa cttccagggg ctgggcggcg atgaagcaaa
    10321 cgcccaggcg caagggattc tggaacgcgc ggcgaagcgt gccggattca gggacgtggt
    10381 attccagtac gagccggtcg cggctgggct ggattacgaa gccaccttgc aggaagaaaa
    10441 acgggtgctg gtggtggata tcggcggtgg tacgactgac tgttcattgc tgctgatggg
    10501 gccgcagtgg cgttcgcgtc tcgatcgtga agccagcctg ctgggtcaca gtggttgccg
    10561 tattggcggt aacgatctgg acatcgcact ggcgtttaaa aacctgatgc cgttactggg
    10621 catgggtggc gaaaccgaaa aaggcatcgc cctgccgatt ctgccgtggt ggaatgcggt
    10681 tgccatcaac gacgtacctg cgcagagtga tttctacagt agtgccaacg gtcgtctgct
    10741 taacgatctg gtacgcgatg cccgcgaacc ggaaaaagtg gccctgctac agaaagtctg
    10801 gcgtgagcgt ttaagctatc gcctggtgcg tagcgcagaa gagtgcaaaa ttgctctttc
    10861 aagcgtagcg gaaacccgcg cctcactgcc gtttatcagc gatgaactgg ctacgctgat
    10921 tagccagcaa gggctggaaa gcgccctcag ccagccactg gcgcggattc tggaacaggt
    10981 gcaactggcc ctggataacg cccaggaaaa accggacgtt atctacctga ccggcggtag
    11041 cgcccgttcg ccgctgatta aaaaagcgct ggcagaacag ttgccgggaa ttccgattgc
    11101 aggcggcgat gactttggct ccgtcaccgc cgggctggca cgctgggcgg aagtggtgtt
    11161 tcgttaaaag aaaatggctc agcagcggct taaatctgcc tacgcctgag ccagtttttc
    11221 tgccgcctga cgtaaaggtt ccatcaacgg catggtccgt gctggagcat gaaggcggaa
    11281 tcgctgcaac tctgtcgcgc cgacagtcaa cgtcgattcc agccgatgtc gtttggcggt
    11341 cattttcgcc attaccgctt gtctgtctgc tggcgttatc gcttcattcg gcctgaacac
    11401 aaaacggcgt tcacagctcg ctttccagtc gataaccgcc tgggtcagat ggtcgccaaa
    11461 ccctttcact tgcttaacgc cacgacgggt aacatctgcg gctgtttcaa taccaaaaga
    11521 ccgtaacgcc gctttacgcg cagggccaac gccgggaata gaggcaacat caataaaaaa
    11581 tccctccaga aacttctgct tctgccgttc ccttgcagtg tcatgaagtg ctgccagagc
    11641 gcgtttctct tcttccggta acccgagaat ttcgtccttc attttttcga gcatcgcccg
    11701 tttggcgata aaaccttcca gcccgcccaa ctgttggatc tggctgacta aatgctcata
    11761 gtccattttc gcgcgattaa atgcctcacg gcgttgctgg atttctgctt tgtacgcttt
    11821 gcttgtcaga ctgccaataa tccagatagc cgacagcaca ggaaccaaaa taatataacg
    11881 cggttctgcc tgaaggccgc aaagcaacag cgataacgct gacagtgcga tctcaatcag
    11941 aatgatgtat tcgcgccgta acaggcctaa aggaagcggc ctgccagtcg gttggaaatg
    12001 atcgggtaat ggcaattgca atgctggcgg tgctactgac gccatcacca tcgcccagac
    12061 tttcgccagc acaaaatcac cgccggtggt aatgacctct tcgccgagat caataaaata
    12121 gataacgcct tgattatcca gcgcacacca cgggcagtcg gtcaaatgag cgggataaac
    12181 atgcattgcc gaaacgggac atttctttaa ctgttggcgc agagaatcaa gtgccgctac
    12241 ccacgcttta gccgtcggac gcccggttgc cacgccactt tccgtgaatg cctgctgaaa
    12301 catggcttca acatcgcccg gtaacatcga tagcggaatc gatcgtggcg gcggttttaa
    12361 accacgtcgc tgattatctg acgcgtaggc ataacggaaa tgggtaatat ccgtctccag
    12421 cgcattaccc gcatcagaga taagcggcac accagaataa ggatgccggc caccaaacaa
    12481 gacgtgaaaa atcagcaacg caaggccaaa attatcgtga ttttctgtgc gttcaaagcc
    12541 gacaaatgac gacaaggttt gtagctctgg cggcgtaaaa tgcgacacgc cgacttcgca
    12601 taaatgcagt gtgccattgg cgttaatctg aaaggagtcg ctatcaatca gtaccacttt
    12661 gctgtcgcga cctaccataa aactgttctg gtttacgtca cccacgacgt gcccgtgctc
    12721 gtgaaccgta gcaaaagatg aagcaatatt gcgcgcaaca tagagtagaa aatcccacgc
    12781 acaatgaggg taacgttgac gacgatgtgc cgggctatag atcatatgaa tcggttcttt
    12841 accagaaact tttggcatca taaaaccgat aacttttccg cctcgtccac cgtgaagcgt
    12901 tgcctgcggc caggcgacat aattcaacaa ttgcgcgtca gctgtcgcag ccataaaggc
    12961 aagtttgtcc tgttttaagg cgggtggcgg cgtgtgataa atcttggcga cgctatcgac
    13021 aaactcattg atatcataaa ccgcgccttc accgcctttg cccagttcac ggcctggcgt
    13081 aacgcactca ccagtagcag tatataaagt gggtttcatg gtaaccacag cgccagtgcc
    13141 aacgttttat catcatccgt acgctcgttt accgccgggc tggacaaaaa ctgctttaac
    13201 aattcaggta ataaatcgag ttgttcctgc gttgctgacg ccagtccatt gaaaaacggg
    13261 gtaaagaaag gtacatgagg agaattatcc agcatattta acgccagtcg ctggatacca
    13321 tccgtaaatg ctgcaacttt atgcacacgc tcagtgctgg tgaaagtttc cagtctggaa
    13381 acggcatctt catcggtaat gaagtgcgtc atattggcat attcacccac catcggcgta
    13441 agcggcaatt gcagaccgtg accaaaatcc accaccacgc cgccatcgcc aatctgcata
    13501 atcaaggtgc cattagccga tgaaatcaat cccagaaacg tgcaggcaaa atcgcgaact
    13561 gctaattctt tggcttccgc ctcggcaaaa agtcgctgac gaacggtcag cacaatatcc
    13621 gtggcgagga tatcattaag ccccaattcc ccaccctgca ctttttgcga catataagcc
    13681 atcgcttcat tgacggcgag cgtcgcgcct tctcctccct gcgaaacgct acctgcgcca
    13741 tcagcaagga acatcaccag taatggctgc tggtcattta gccaggcaac ctgcatctgg
    13801 caggcatcct ggcaaggcaa gtccgcgctg atgtgcgatg ttccaacagc ggatgcataa
    13861 accagacgcc agctcacact gacgtccagc cttttggcgc ttccagcaca acttccgttc
    13921 ccggcgtgga gcgggaaacc gaacgcagcg aactggataa ccagctaaac agttcacgga
    13981 attgtaatcc ttgcagaggc aaaggctgac gaacgctgat ttgtgccaga gtcttcatat
    14041 ccgccccctg aacaccgatg gaaaagaaag caaacctttt atcttcttcc ccccgaaaca
    14101 ctttgttggc tgcagcctgc cactcatcgg ttggtgcacc atcggtaatc aggaaaatcc
    14161 acgggcgata atacgaaata ccatttgcac gatactcacg tttccgctcc tctaccatat
    14221 ccagggcttt ggtaatggcc gcacccattg gtgtatcgcc ctgggcaaac aggatgggcg
    14281 gaaaaaaatt agcggcgctg gtaaagggtt gttccacatg caccgggccg aacgtcacaa
    14341 tgccgagttc cactcttttt aaagccagcg gatcggcaag cagttcatca cgaaaggtca
    14401 ccaatccggc gttaagttca ttaataggtc tgccgctcat cgagccagaa acatccagca
    14461 gcaaaatgca ggggcaacgt ggttccgggt tactggcaaa atcgctggtg gcaaacgtga
    14521 tttgttcgct cataagaaat cctttcttag agaattatcg aggtccggta tcataccgac
    14581 tgcaataatg aaataagagc aaacgaagaa actttcagaa ttaggcccgg cggcgcagaa
    14641 aattttgcta ttcgcctcgc aagaacggag agccgtcgat tgacactaag ggcggagtga
    14701 cataatttca ggagtgaggg ttagggagag gtttccccct ccccctggtg ttcttagtaa
    14761 gcctggaagc taatcactaa gagtatcacc agtatgatga cgtgcttcat cataaccctt
    14821 tccttattaa aagccctctt ctccgggaga ggctttcccg tttcagcgtc ccgctgaaat
    14881 catcggctta cctcctttcg ccctgaatgc agtctatcgc taacgcgtga ataaaatagt
    14941 ttcctctgtg attattgcgt aacgactcgc aaagaatatt tgctggcagg atcgcagact
    15001 acaaagcctg cggattgaca atcttatcgt gaaggcatac tttcaggagt gagggtagag
    15061 cggggtttcc cccgccctgg tagtcttagt aagcggggaa gcttatgact aagagcacca
    15121 cgatgatgag tagcttcatc atgacccttt ccttatttaa ggccccttcc tcgggagggg
    15181 ctttcccgtt tcagcgtccc gctgaaatcg tcggcttacc tcctttcgcc atgcacttag
    15241 tctatcgctg acttgcagat aaaatagttt ccagtgtgat tattgcgtaa cgtctcgcaa
    15301 agaatttttg ctggcaggat cgcagacggc aaagcctgcg gattgacaat cttatcgtga
    15361 aggcatactt tcaggagtga gggtagagcg gggtttcccc cgccctggta gtcttagtat
    15421 gctgggtagc taatcactaa gagcaccacg atgatgagta gcttcatcat gaccctttcc
    15481 ttatttaagg ccccttcctc gggaggggct ttcccgtttc agcatccggc tgaaatctgc
    15541 ggcttacctc ctttcgccct gtatgcagtc tatcgctaac gcgtagataa aatagtttcc
    15601 tgtgttatta ctggatgcgt gctcgcaaat gtgcccgcca ttcagacgat tcccgatagt
    15661 gtttcataat tcctccattt ttctccctta ttggctggct acactagtat cattccgcga
    15721 aacgtttcag gaagagaaac tcttaacgat gaaaggcagt tataaatccc gttgggtaat
    15781 cgtaatcgtg gtggttatcg ccgccatcgc cgcattctgg ttctggcaag gccgcaatga
    15841 ctcccggagt gcagccccag gggcgacgaa acaagcgcag caatcgccag cgggtggtcg
    15901 ccgtggtatg cgttccggcc cattagcccc ggttcaggcg gcgaccgccg tagaacaggc
    15961 agttccgcgt tacctcaccg ggcttggcac cattatcgcc gctaatactg ttacggtgcg
    16021 cagccgcgtg gacggtcaac tgatggcgtt acatttccag gaaggccagc aggtcaaagc
    16081 aggcgattta ctggcagaaa ttgaccccag ccagttcaaa gttgcattag cacaaaccca
    16141 gggccaactg gcaaaagata aagccacgct tgctaacgcc cgccgtgatc tggcgcgtta
    16201 tcagcaactg gcaaaaacca atctcgtctc ccgtcaggag ctggatgccc aacaggcgct
    16261 ggtcagtgaa accgaaggca ccattaaggc tgatgaagca agcgtcgcca gcgcgcagtt
    16321 gcaactcgac tggagccgca tcaccgcacc agtcgatggt cgcgttggtc tcaagcaggt
    16381 tgatgttggt aaccaaatct ccagtggtga taccaccgga attgtggtga tcacccagac
    16441 gcatcctatc gatttagtct ttaccctgcc ggaaagcgat atcgctactg tagtgcaggc
    16501 gcaaaaagcc ggaaaaccgc tggtggtaga agcctgggat cgcaccaatt cgaagaaatt
    16561 aagtgaaggc acgctgttaa gcctcgataa ccaaatcgat gccacaaccg gtacgattaa
    16621 agtgaaagca cgctttaata atcaggatga tgcgctgttt cccaatcagt ttgttaacgc
    16681 gcgcatgtta gtcgacaccg aacaaaacgc cgtagtgatc ccaacagccg ccctgcaaat
    16741 gggcaatgaa ggccattttg tctgggtgct gaatagcgaa aacaaggtca gcaaacatct
    16801 ggtgacgccg ggcattcagg acagtcagaa agtggtgatc cgtgcaggta tttctgcggg
    16861 cgatcgcgtg gtgacagacg gcattgatcg cctgaccgaa ggggcgaaag tggaagtggt
    16921 ggaagcccag agcgccacca ctccggaaga gaaagccacc agccgcgaat acgcgaaaaa
    16981 aggagcacgc tcctgatgca ggtgttaccc ccgagcagca caggcggccc gtcgcgcctg
    17041 tttattatgc gtcctgtctc caccacgctg ctgatggtgg cgatcttact cgccgggatt
    17101 atcggttatc gcgccctgcc cgtttcggcg ctgccggaag tggactatcc gaccattcag
    17161 gtggtcacgc tctacccagg tgccagcccg gatgtcatga cctctgccgt taccgcgccg
    17221 ctcgaacgcc agttcgggca gatgtctggc ctgaaacaga tgtcgtcgca aagttccggc
    17281 ggtgcgtcag ttatcacttt gcagttccag ctaacattac cgctcgatgt cgccgagcag
    17341 gaagtgcagg ccgcgattaa cgctgcgacc aacttgttgc cgagcgatct gcctaacccg
    17401 ccggtttaca gcaaagtgaa cccggcagat ccgccgatca tgacgctcgc cgtcacctca
    17461 accgccatgc cgatgacgca agtggaagat atggtggaaa cccgcgtcgc gcagaaaatc
    17521 tcgcagattt ccggcgtcgg cctggtgacg ctttccggcg gtcagcgtcc ggctgttcgc
    17581 gtcaaactta acgctcaggc gattgccgcc ctcggcctga ccagcgaaac cgtgcgcacc
    17641 gccattaccg gcgctaacgt taactcggca aaaggtagcc tcgacggccc ttcccgtgcg
    17701 gtcacgcttt ccgcgaacga ccagatgcaa tccgccgaag agtatcgcca gctaatcatc
    17761 gcctaccaga acggcgcgcc aattcgtctg ggcgatgtcg caactgtaga gcaaggtgca
    17821 gaaaacagct ggctcggcgc gtgggcgaac aaagaacagg ccattgtgat gaatgttcag
    17881 cgccagcccg gtgctaacat tatctccacc gccgacagca ttcggcagat gctgccacag
    17941 ctcactgaga gtctgccgaa atcggtgaag gtgacagtgc tttccgatcg caccaccaat
    18001 atccgcgcat ccgtcgatga tactcagttt gaattgatga tggctatcgc gctggtagtc
    18061 atgattatct acctgttttt gcgcaatatt ccggcgacca tcattcccgg tgttgctgta
    18121 ccgctgtcgt taatcggcac tttcgcggtt atggtgtttc tcgatttttc aatcaataac
    18181 ctgacactga tggcgttaac tatcgccacc ggattcgtgg tcgatgacgc catcgtggtg
    18241 atcgaaaaca tttcccgcta tatcgaaaaa ggcgaaaaac cgttggcggc ggcgctcaag
    18301 ggcgcaggtg aaatcggctt taccattatc tcgctgacct tctcactgat tgcggtgttg
    18361 atcccactgc tgtttatggg cgatatcgtc gggcgactgt tccgcgaatt tgctattacg
    18421 ctggcggtag cgattttgat ctcagcggtg gtgtcgctga ccctgacacc gatgatgtgc
    18481 gcgcggatgc tcagccagga gtcgttgcgt aaacagaacc gcttctcccg tgcctcggaa
    18541 aaaatgttcg acaggataat cgccgcctat ggtcgtggac tggcgaaggt gctgaatcat
    18601 ccgtggctga ccttaagcgt ggcgctcagc acgctgctgc ttagcgttct cttatgggtg
    18661 ttcattccga aaggtttctt cccggtacag gacaacggca ttattcaggg cactttgcag
    18721 gcaccgcaat ccagctcctt tgccaatatg gcccagcgac aacgccaggt cgcggacgtg
    18781 attttgcagg atccggcagt gcaaagcctg acctcatttg ttggcgttga tggcactaac
    18841 ccgtcgctga atagtgcacg tttacaaatc aacctcaaac cgctggatga acgtgatgac
    18901 cgagtgcaaa aagtcattgc ccgtctgcaa acggcggtgg ataaagtgcc gggcgtcgat
    18961 ctcttcctgc aaccaacgca ggatctgact attgatactc aggtcagccg cacccagtac
    19021 cagtttacct tgcaggccac ctcactggat gcgctcagta cctgggtgcc acagttgatg
    19081 gaaaaactcc agcaactgcc acagctttct gatgtctcca gcgactggca ggacaaaggg
    19141 ctggtggcgt atgtcaatgt tgatcgcgac agcgccagcc gtctggggat cagcatggcg
    19201 gatgtcgata acgccctgta caacgcgttt ggtcagcgac tgatttccac tatttatact
    19261 caggctaacc agtatcgcgt agtgctggag cacaacaccg aaattacccc gggcctcgcg
    19321 gcgctggata ccattcgcct gaccagtagc gacggcggcg tggtgccgtt aagctcaatt
    19381 gccaaagttg aacagcgttt tgctccgctt tccatcaacc atctggatca gttcccggta
    19441 acgaccatct cctttaacgt gccggataac tattcgctgg gcgatgcggt gcaggcgatt
    19501 atggacaccg aaaagacgct gaatctgccg gtggatatca ccacgcaatt tcagggcagc
    19561 accctcgcct tccagtcggc gctgggcagc actgtctggc tgattgtcgc ggcggtggtg
    19621 gcgatgtata tcgtgctcgg cattctgtac gagagcttta ttcacccgct caccattctc
    19681 tcgacgctac ccaccgcagg ggtcggcgcg ctgctggcat taatgattgc cggtagcgaa
    19741 ctggatgtga ttgcgattat cggcattatt ttgctgatcg gtattgtgaa gaagaacgcc
    19801 atcatgatga tcgacttcgc gctggctgct gagcgcgagc aaggcatgtc gccgcgcgat
    19861 gcaatctacc aggcttgtct gttgcgtttt cgtccgatcc tgatgaccac cctggcggct
    19921 ctgcttggcg cgctgccgct gatgttgagt accggggtcg gcgcggaact gcgtcgtccg
    19981 ttaggtatcg gcatggtcgg cggtctgatt gtcagccagg tgctgacgct gttcaccacg
    20041 ccggtgattt atctgctgtt cgaccgcctg gcattgtgga ccaaaagtcg ctttgctcgc
    20101 catgaagagg aggcgtaagt gaagtttttt gccctcttca tttaccgccc ggtggcgacg
    20161 attttactgt cggtcgccat taccctgtgc ggcatactgg gcttccgtat gctgccggtc
    20221 gccccgctgc cgcaggtcga ttttccggtg attatggtca gcgcctcgct gcccggtgcg
    20281 tcaccagaaa caatggcgtc ttccgttgcc acgccgctgg agcgctcact tgggcgcatt
    20341 gccggagtca gtgaaatgac ctccagcagt tcgctcggca gcacgcgtat tattttgcag
    20401 tttgattttg accgggatat caacggcgcg gcgcgcgatg tgcaggcggc gatcaacgct
    20461 gcacaaagtt tgctgcccag cgggatgccc agccgtccga cctatcgcaa agctaacccg
    20521 tcggatgcgc caattatgat cctcacgctg acatccgata cttattcgca gggtgaactg
    20581 tacgattttg cctcgacgca gctggctccg acgatttcgc aaatcgacgg tgtcggtgat
    20641 gtcgatgttg gcggcagctc gctgcccgcc gtacgcgtcg ggctgaatcc gcaggcgctg
    20701 tttaatcagg gcgtctcgct ggacgacgta cgcaccgcca tcagcaatgc caacgtgcgt
    20761 aaaccgcagg gggcgctgga agatggcact caccgctggc agatccagac caatgatgag
    20821 ctaaaaaccg ccgccgaata tcagccgttg attattcatt acaacaacgg cggcgcggtt
    20881 cgtctgggcg atgtggcgac ggtgaccgac tcggtgcagg atgtgcgcaa cgccgggatg
    20941 accaacgcca aaccggctat tttgctgatg atccgcaagc tgccggaagc caatattatc
    21001 cagacggtag acagcatccg ggcaaaatta ccggagttgc aggaaaccat tccggcggcg
    21061 attgatctgc aaattgctca ggatcgctcc cccaccattc gcgcctcgct ggaagaagtc
    21121 gagcaaacgc tgattatctc ggtggcgctg gtgattctgg tggtgttttt attcctgcgc
    21181 tcgggtcgcg ccactattat tcccgccgtt gcggtgccgg tttcgctgat tggtacgttt
    21241 gcggcgatgt acctgtgcgg attcagtctc aataaccttt cgttaatggc gctcaccatc
    21301 gctactggtt tcgtggtgga tgacgccatc gtggtgctgg aaaacattgc ccgtcatctg
    21361 gaagcgggga tgaaaccgct acaggccgcg ctacaaggta ctcgcgaagt cggctttacg
    21421 gtgctatcga tgagtctgtc actggtggcg gtgttcctgc cgctgctgtt gatgggcgga
    21481 ttgccgggcc gactgttacg cgaatttgcc gtgacgcttt ctgtcgccat tgttatatcg
    21541 ttgctggttt ctctgacatt aacgccaatg atgtgtggct ggatgctgaa agccagcaag
    21601 ccgcgtgagc aaaagcgact gcgtggtttt ggccgcatgt tggtagccct gcaacaaggc
    21661 tacggcaagt cgctgaaatg ggtgctcaat catacccgtc tggtgggtgt ggtgctgctt
    21721 ggcaccattg cgctgaatat ctggctgtat atctcgatcc cgaaaacctt cttcccggag
    21781 caggacaccg gcgtgctgat gggcgggata caggcggatc agagcatttc gtttcaggcg
    21841 atgcgcggca aattgcagga tttcatgaaa attatccgtg acgatccggc agtggataat
    21901 gtcaccggct ttaccggcgg ttcacgagtt aacagtggga tgatgtttat caccctcaag
    21961 ccacgcgacg aacgcagcga aacggcgcag caaattatcg accgtctgcg cgtaaaactg
    22021 gcgaaagaac cgggggcgaa tctgttcctg atggcggtac aggatattcg cgttggtggg
    22081 cgtcagtcga acgccagcta ccagtacacg ttgttatccg acgacctggc ggcactgcgc
    22141 gaatgggagc cgaaaatccg caaaaaactg gcgacgttgc cggaactggc ggacgtgaac
    22201 tccgatcagc aggataacgg cgcggagatg aatctggttt acgaccgtga caccatggca
    22261 cggctgggaa tcgacgtaca agccgccaac agtctgttaa ataacgcctt cggtcagcgg
    22321 caaatctcga ccatttacca gccgatgaac cagtataaag tggtgatgga agtggatccg
    22381 cgctataccc aggacatcag tgcgctggaa aaaatgttcg ttatcaataa cgaaggcaaa
    22441 gcgatcccgc tgtcgtattt cgctaaatgg caaccggcga atgccccact atcggtgaat
    22501 catcagggat tatcggcggc ctcgaccatt tcgttcaacc tgccgaccgg aaaatcgctc
    22561 tcggacgcca gtgcggcgat cgatcgcgca atgacccagc ttggtgtgcc ttcgacggtg
    22621 cgcggcagtt ttgccggaac agcgcaggtg ttccaggaga cgatgaactc acaggtgatc
    22681 ctgattatcg ccgccatcgc cacggtgtat atcgtgctgg gtatccttta cgagagttat
    22741 gtgcacccgc tgacgattct ctccaccctg ccctcggcgg gtgttggagc gctgctggcg
    22801 ctggagctgt tcaatgcccc gttcagccta atcgccctga tagggatcat gctattaatc
    22861 ggcatcgtga agaaaaacgc cattatgatg gtcgattttg cgcttgaagc ccaacggcac
    22921 ggtaacctga cgccgcagga agctattttc caggcctgtc tgctgcgttt tcgcccgatt
    22981 atgatgacta ccctggcggc gctgtttggc gcgctgccgc tggtgttgtc gggcggcgac
    23041 ggctcggagc tgcggcaacc cctggggatc accattgtcg gcggactggt aatgagccag
    23101 ctccttacgc tgtataccac gccggtggtg tatctctttt tcgaccgtct gcggctgcgt
    23161 ttttcgcgta aacctaaaca aacggtaacc gagtaaatga cagatcttcc cgacagcacc
    23221 cgttggcaat tgtggattgt ggctttcggc ttctttatgc agtcgctgga caccaccatc
    23281 gtaaacaccg cccttccctc aatggcgcaa agcctcgggg aaagtccgtt gcatatgcac
    23341 atggtcattg tctcttatgt gctgaccgtg gcggtgatgc tgcccgccag cggctggctg
    23401 gcggacaaag tcggcgtgcg caatattttc tttaccgcca tcgtgctgtt tactctcggt
    23461 tcactgtttt gcgcgctttc cggcacgctg aacgaactgt tgctggcacg cgcgttacag
    23521 ggcgttggcg gcgcgatgat ggtgccggtc ggcagattga cggtgatgaa aatcgtaccg
    23581 cgcgagcaat atatggcggc gatgaccttt gtcacgttac ccggtcaggt cggtccgctg
    23641 ctcggtccgg cgctcggcgg tctgctggtg gagtacgcgt cgtggcactg gatctttttg
    23701 atcaacattc cggtggggat tatcggtgcg atcgccacat tgatgttaat gccgaactac
    23761 accatgcaga cgcggcgctt tgatctttcc ggctttttat tgctggcggt tggcatggcg
    23821 gtattaaccc tggcgctgga cggcagtaaa ggtacaggtt tatcgccgct ggcgattgct
    23881 ggcctggtcg cagttggcgt ggtggcactg gtgctttatc tgctgcacgc cagaaataac
    23941 aaccgcgccc tgttcagtct gaaactgttc cgcactcgta ccttttcgct gggcctggcg
    24001 gggagctttg ccggacgtat tggcagtggc atgttgccct ttatgacacc ggttttcctg
    24061 caaattggcc tcggtttctc gccgtttcat gccggactga tgatgatccc gatggtgctt
    24121 ggcagcatgg gaatgaagcg aattgtggtt caggtggtga atcgctttgg ttatcgtcgg
    24181 gtactggtag cgaccacgct gggtctgtcg ctggtcaccc tgttgtttat gactaccgct
    24241 ctgttgggct ggtactacgt tttgccgttc gtcctgtttt tacaaggaat ggtcaactca
    24301 acgcgtttct cctccatgaa caccctgacg ctgaaagatc tcccggacaa tctggcgagc
    24361 agcggaaaca gcctgctgtc gatgattatg caattgtcga tgagtatcgg cgtcaccatc
    24421 gccgggctgt tgctgggact ttttggttcg cagcatgtca gcgtcgacag cggcaccaca
    24481 caaaccgtct ttatgtacac ctggcttagc atggcgttga tcatcgccct tccggcgttc
    24541 atctttgcca gagtgccgaa cgatacgcat caaaatgtag ctatttcgcg gcgaaaaagg
    24601 agcgcgcaat gaagttctgg cgacccggta ttaccggcaa actgtttctg gcgattttcg
    24661 ccacctgcat tgtcttgctg atcagtatgc actgggcggt gcgtatcagt tttgagcgtg
    24721 gctttattga ttacatcaag catggtaatg aacaacgatt acaactgtta agtgatgcgc
    24781 ttggcgagca gtatgcgcag catggcaact ggcgcttcct gcgcaacaat gatcgctttg
    24841 tctttcagat cctgcgttca tttgaacacg ataattcgga agataaaccc ggcccgggta
    24901 tgccaccgca cggctggcgt acccagttct gggtggttga tcaaaacaac aaagtgctgg
    24961 ttggtccgcg agcgccgatt ccacctgacg gtacacggcg acccattctg gtcaacggtg
    25021 cggaagttgg cgcggtgatc gcctcccccg ttgagcggtt aacgcgcaat actgatatca
    25081 atttcgataa acaacagcgg caaaccagct ggctgatcgt cgccctggca acgttactcg
    25141 cggcgctcgc cacctttctg ctggcgcgcg gtttgctggc accggtaaaa cgacttgtcg
    25201 atggcacgca caaactggcg gcgggcgatt tcactacccg cgtaacgccc accagtgaag
    25261 atgaactggg caaactggcg caagacttca accagcttgc cagcacgctg gagaaaaacc
    25321 agcaaatgcg gcgcgatttt atggccgata tttcacacga actgcgtacg ccattagcgg
    25381 tgctgcgcgg tgaactggaa gccattcaag atggcgtgcg caaattcacg ccggagacgg
    25441 tggcgtcttt acaggcggaa gtcggtacac tgactaaact ggttgacgat cttcatcagt
    25501 tgtcgatgtc tgatgaaggc gctctcgcct atcaaaaagc accggtagat ttgatcccac
    25561 tgctggaagt ggcgggcggc gcatttcgcg aacgatttgc cagccgtggc ctgaaactgc
    25621 agttttccct gccagacagt attaccgtat ttggcgatcg cgaccgttta atgcagttat
    25681 tcaataactt actcgaaaac agcctgcgct acactgatag cggcggcagc ctgaaaatct
    25741 ctgccgagca gcacgacaaa acggtgcgcc tgacctttgc cgacagtgcg ccaggtgtca
    25801 gtgacgagca gctacaaaaa ttatttgaac gtttttatcg caccgaaggt tcccgcaacc
    25861 gcgccagcgg tggttccggg ctggggctgg cgatttgcct gaacattgtt gaagcacata
    25921 atggccgcat tattgctgcc cattcgcctt ttggcggggt aagcattaca gtagagttac
    25981 cgctggaacg ggatttacag agagaagtat gaccgagtta ccaatcgacg aaaacacacc
    26041 gcgtattttg atcgtggaag atgaaccgaa gctggggcag ttgctcattg attatctgcg
    26101 tgctgcgagc tatgcgccga cgcttatcag ccacggcgat caggtactgc cgtatgtgcg
    26161 ccagacacca ccggatctga tcctgttaga tctgatgctc cctggcaccg atggcctgac
    26221 gctgtgccgg gaaattcgtc gtttttctga cattccgatc gtgatggtga cggcaaaaat
    26281 cgaagagatc gatcgcctgc tggggctgga gattggcgca gatgattata tctgtaagcc
    26341 gtacagccca cgggaagtgg tagcgcgcgt caaaaccatt ttgcgccgtt gcaaaccgca
    26401 gcgcgagttg cagcaacagg atgctgaaag cccgttgatt atcgacgaag gtcgttttca
    26461 ggcttcatgg cgcggtaaaa tgcttgacct gacgcctgcg gaatttcgtc tgctgaaaac
    26521 gctctctcat gaaccaggaa aagtgttctc ccgcgagcat ttgctcaatc atctttatga
    26581 cgactaccgc gtagtaaccg accgcaccat cgacagccac attaaaaacc tgcgccgcaa
    26641 gctggaatct ctcgacgccg aacagtcatt tatccgcgcc gtttacggcg ttggttaccg
    26701 ctgggaagcc gacgcctgcc gcatcgttta gttttagcga catgactttg ttagccggag
    26761 atggcgttca gaccagatcc ggcaacatta tcccacgcat ggtcagcaaa ctgacattat
    26821 ctctccgccc tggcaggcat cagaaaatcg ctcatacttt aatcggtaaa cagcaccttt
    26881 agatgctgtt ttcgatacgc aatttcaatc aaggagtcat tatggctggt tggtttgaac
    26941 tcagtaagag cagtgataat cagttccggt ttgtgctaaa agcgggcaat ggtgagacta
    27001 tcctcaccag cgagctgtat acctcaaaag cctctgcgga aaagggcatc gcgtcggtgc
    27061 gtagcaacag cccgcaagaa gaacgctatg agaaaaaaac ggcaagtaac ggcaaattct
    27121 atttcaatct gaaagccgct aatcatcaaa ttatcggctc cagccagatg tacgccaccg
    27181 cgcaatctcg tgaaaccgga attgcctccg ttaaagccaa tggcacaagc cagacggtga
    27241 aagacaatac gtaattacga tgccgggcgt gggttgttgg cgtccggctt tatatccaaa
    27301 taacgcttag ttattaataa ttaagagtaa ccgccgccat taaaatagtg ggcactgacg
    27361 agcactcgtt gtgctttctt tgtttaccat cccatactta tcctcctgaa catgttcatg
    27421 tgccaaaata tcttctaata ctttcgtgac gctatccggt ttatgacata cactgatgta
    27481 agatttttac ttacaggcgg gcgtaaaatg gccagaacga tgacggttga tctcggcgat
    27541 gagttgcgtg aatttatcga gtccttaatt gaatccggcg actatcgcac ccaaagcgaa
    27601 gtgatccgtg agtcactgcg attacttcga gaaaagcaag cagagtctcg cctgcaagcg
    27661 ttacgtgaca tgctcgcaga agggttgagt agtggtgaag ctcagccgtg ggaaaaggac
    27721 gcgttcttac ggaaggtgaa agcagggata aggaaataat gtgcgtatta ttaaactcat
    27781 gccaaaagcc aatgaggatt tagaaggcat ctggtattac agctatcacc atttcggcga
    27841 accacaagcc gacagatatg tggaacatct ttcagacgtt ctccagattc tgagtaacaa
    27901 taatataggt acgcccagac ctgaattagg cgaaggtatt tttgttcttc cttttgaacg
    27961 ccacgtcatt tatttcctgc aatcaccagg ggaaattatc gtcattcgta tccttaacca
    28021 aaaccaggac gccaccagac atttacactg gagctaaacc cgccgattgc gctacaatgc
    28081 ccgcccttaa agtgggggca ctcccctaac cgcttcatca ggtgaagcgg atctgacctg
    28141 tcatcagaac gagagaatta tgtttaaacc ggaactcctt tccccggcgg gaacgctgaa
    28201 aaatatgcgt tacgctttcg cttatggcgc agatgctgtt tatgcgggcc agccgcgtta
    28261 ttccctgcgt gtgcgcaaca acgaattcaa ccacgaaaat cttcagctcg gcatcaatga
    28321 agcccacgcg ctggggaaaa agttttatgt cgtggtcaac attgcaccgc acaacgccaa
    28381 gctgaaaacc tttatccgtg acctgaaacc ggtggtggaa atggggccgg atgcgctgat
    28441 tatgtccgat ccagggctga ttatgctggt gcgtgagcac ttccctgaaa tgccgatcca
    28501 cctttcggtg caggctaacg ccgtgaactg ggcgacggtg aaattctggc agcaaatggg
    28561 cctgacccgc gtgatcctct ctcgcgagct gtcgctggaa gagattgaag agatccgcaa
    28621 tcaggtgccg gatatggaga tcgagatctt cgttcacggc gcgctgtgca tggcctactc
    28681 cggtcgctgc ctgctctctg gctatatcaa caagcgcgac ccgaaccagg gcacctgcac
    28741 caacgcctgc cgctgggagt acaacgtcca ggaagggaaa gaagatgatg ttggcaacat
    28801 cgtacacaag tacgagccga ttccggtgca aaatgttgag ccgacgctgg gtatcggcgc
    28861 accaaccgac aaagtgttta tgatcgaaga ggcccagcgt ccgggcgagt atatgaccgc
    28921 gtttgaagat gagcacggca cttacatcat gaactcgaaa gatctgcgcg ccatcgccca
    28981 tgtagaacgc ctgaccaaaa tgggcgtgca ttcgctgaaa atcgaaggtc gtaccaaatc
    29041 tttctactat tgtgcacgca ccgcacaggt ttaccgcaaa gctatcgatg acgccgctgc
    29101 gggaaaaccg ttcgatacca gcctgctgga aactctggaa ggtctggcgc atcgtggcta
    29161 taccgaaggt ttcctgcgtc gtcatactca cgacgattat cagaactacg aatacggtta
    29221 ttcagtttct gaccgccagc agtttgttgg tgagtttacc ggtgagcgca agggggacct
    29281 cgcggcggta gcggtgaaaa ataaattctc cgttggcgac agccttgagc tgatgacgcc
    29341 gcaaggcaac attaatttta cccttgagca catggaaaac gccaaaggcg aagctatgcc
    29401 gatagcacca ggcgatggtt atactgtgtg gctcccggtc ccgcaggatc ttgagctcaa
    29461 ttacgcgctg ctgatgcgta atttctccgg ggaaaccacg cgtaatcccc acggtaagtg
    29521 attaatttcg attatttttc ccggatggaa aattcttaga aaccgatcac atacagctgc
    29581 atttattaag gttatcatcc gtttcgctga aaaacataac ccataaaatg ctagctgtac
    29641 caggaaccac ctccttagcc tgtgtaatct cccttacacg ggcttatttt ttatgcataa
    29701 gccctatccc tggtcaccgt cttccattga ccacatcgat agaatctccc ttcatagcac
    29761 gatgcctttc acgtaacggc atcgtgctcg cacaggttcc ggctaagcac aaccagaacg
    29821 cgcatgtttg acgcttacca aaaaatattc tcactctcca catttgaatg tcagacgagc
    29881 gacgccatgt aatcctgcac cttctgtctt caggtcaact atctgcattt ttttgccctg
    29941 agtaacacag aaatgggctg catcattttt tactatattt tctgcaccag atattctgcc
    30001 cctggctaaa gaagcttcgg cttcggtgta gtattggtta tcgagtttac gctgaatatt
    30061 acttttatat gcaagaccaa atttaccgat
//
LOCUS       DAZKHF020000028        30051 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2201S31241
            SAMN43267800-rid25106413.denovo.028, whole genome shotgun sequence.
ACCESSION   DAZKHF020000028 DAZKHF020000000
VERSION     DAZKHF020000028.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267800
            Sequence Read Archive: SRR30307263
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 30051)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 30051)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 30051)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350581.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350581.2
            Genome Coverage       :: 151x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:21:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,652
            CDSs (total)                      :: 5,557
            Genes (coding)                    :: 5,320
            CDSs (with protein)               :: 5,320
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 237
            CDSs (without protein)            :: 237
            Pseudo Genes (ambiguous residues) :: 0 of 237
            Pseudo Genes (frameshifted)       :: 102 of 237
            Pseudo Genes (incomplete)         :: 130 of 237
            Pseudo Genes (internal stop)      :: 52 of 237
            Pseudo Genes (multiple problems)  :: 41 of 237
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..30051
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267800-rid25106413.denovo.028"
                     /serotype="O 26:H11"
                     /isolate="2201S31241"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-01"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            57..1334
                     /gene="yggP"
                     /locus_tag="ACHOSC_RS05145"
                     /old_locus_tag="pgaptmp_001029"
     CDS             57..1334
                     /gene="yggP"
                     /locus_tag="ACHOSC_RS05145"
                     /old_locus_tag="pgaptmp_001029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding dehydrogenase"
                     /protein_id="HGU4547011.1"
                     /translation="MKTKVAAIYGKRDVRLREFELPEITDNELLVSVISDSVCLSTWK
                     AALLGSEHKRVPDDLENHPVITGHECAGVIVEVGKNLTGKYKKGQRFVLQPAMGLPSG
                     YSAGYSYEYFGGNATYMIIPEIAINLGCVLPYHGSYFAAASLAEPMCCIIGAYHANYH
                     TTQYVYEHRMGVKPGGNIALLACAGPMGIGAIDYAINGGIQPSRVVVVDIDDKRLAQV
                     QKLLPVDLAASKGIELVYVNTKGMSDPVQTLRALTGDVGFDDIFVYAAVPAVVEMADE
                     LLAEDGCLNFFAGPTDKNFKVPFNFYNVHYNSTHVVGTSGGSTDDMKEAIALSATGQL
                     QPSFMVTHIGGLDAVPDTVLNLPDIPGGKKLIYNGVTMPLTAIADFAEKGKTDPLFKE
                     LARLVEETHGIWNEQAEKYLLAQFGVDIGEAAQ"
     gene            1331..2296
                     /gene="glpX"
                     /locus_tag="ACHOSC_RS05150"
                     /old_locus_tag="pgaptmp_001030"
     CDS             1331..2296
                     /gene="glpX"
                     /locus_tag="ACHOSC_RS05150"
                     /old_locus_tag="pgaptmp_001030"
                     /EC_number="3.1.3.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042132 - fructose 1,6-bisphosphate
                     1-phosphatase activity [Evidence IEA];
                     GO_process: GO:0006071 - glycerol metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class II fructose-bisphosphatase"
                     /protein_id="HGU4547012.1"
                     /translation="MMSLAWPLFRVTEQAALAAWPQTGCGDKNKIDGLAVTAMRQALN
                     DVAFRGRVVIGEGEIDHAPMLWIGEEVGKGDGPEVDIAVDPIEGTRMVAMGQSNALAV
                     MAFAPRDSLLHAPDMYMKKLVVNRLAAGAIDLSLPLADNLRNVARALGKPLDKLRMVT
                     LDKPRLSAAIEEATQLGVKVFALPDGDVAASVLTCWQDNPYDVMYTIGGAPEGVISAC
                     AVKALGGDMQAELIDFCQAKGDYTENRQIAEQERKRCKAMGVDVNRVYSLDELVRGND
                     ILFSATGVTGGELVNGIQQTANGVRTQTLLIGGADQTCNIIDSLH"
     gene            2318..2827
                     /gene="fumE"
                     /locus_tag="ACHOSC_RS05155"
                     /old_locus_tag="pgaptmp_001031"
     CDS             2318..2827
                     /gene="fumE"
                     /locus_tag="ACHOSC_RS05155"
                     /old_locus_tag="pgaptmp_001031"
                     /EC_number="4.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarase E"
                     /protein_id="HGU4547013.1"
                     /translation="MATLTEDDVLEQLDAQDNLFSFMKTAHSILLQGIRQFLPSLFVD
                     NDEEIVEYAVKPLLAQSGPLDDIDVALRLIYALGKMDKWLYADITHFSQYWHYLNEQD
                     ETPGFADDMTWDFISNVNSITCNATLYDALKAMKFADFAVWSEARFSGMVKTALTLAV
                     TTTLKELTP"
     gene            2824..3537
                     /locus_tag="ACHOSC_RS05160"
                     /old_locus_tag="pgaptmp_001032"
     CDS             2824..3537
                     /locus_tag="ACHOSC_RS05160"
                     /old_locus_tag="pgaptmp_001032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside/nucleotide kinase family protein"
                     /protein_id="HGU4547014.1"
                     /translation="MKIELTVNGLNVQAQYHDEEIERVHKPLLRMLAALQTVSPQRRT
                     VVFLCAPPGTGKSTLTTFWEYLAQQDPELPAIQTLPMDGFHHYNSWLDAHQLRPFKGA
                     PETFDVAKLAENLCQVVEGDCTWPQYDRQKHDPVEDALHVTAPLVIVEGNWLLLDDEK
                     WCQLAQFCDFSIFINAPATALRERLVGRKLAGGVSLADAEAFYDRTDGPNVRRGLEES
                     LPANLTLMMTATGEYRLVD"
     gene            complement(3509..4186)
                     /locus_tag="ACHOSC_RS05165"
                     /old_locus_tag="pgaptmp_001033"
     CDS             complement(3509..4186)
                     /locus_tag="ACHOSC_RS05165"
                     /old_locus_tag="pgaptmp_001033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708691.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HGU4547015.1"
                     /translation="MLTLNKISYRWPGAATDCLCDISLQLKQGEWLALTGDNGAGKST
                     LLRVMAGLLTPTAGTVMLQQQAMKNLKNRQRAAKVGVLFQEAENQLFHSTVADEIAFG
                     LKLQKCPADEITQRTHAALQCCQLADTANSHPLDLHSAQRRMVAVACLEALSPPLLLL
                     DEPSRDFDENWLSVFESWLEKCGQRGTSVVAISHDAAFTRRHFSRVVRLEDGLIRNVN
                     PPDDIHP"
     gene            complement(4180..4857)
                     /locus_tag="ACHOSC_RS05170"
                     /old_locus_tag="pgaptmp_001034"
     CDS             complement(4180..4857)
                     /locus_tag="ACHOSC_RS05170"
                     /old_locus_tag="pgaptmp_001034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HGU4547016.1"
                     /translation="MVTLEQFRYCPTHSTHPPFCYDFHYVKPGMVAIFGDNGSGKSTL
                     AQLMAGWYPDFLPGEITGTGTLLGTPIGHLPLNEQSATIQLVQQSPYLQLSGCTFSVE
                     EEVAFGPENLCLAEAEIMARIDAALTLTECQPLRHRHPATLSGGETQRVVIACAIAMQ
                     PKLLILDEAFSRLTPQASEMLLQRLQHWAFERGSLIILFERHRTPFLNYCQQAWQLQN
                     GALQPLC"
     gene            complement(4845..5552)
                     /locus_tag="ACHOSC_RS05175"
                     /old_locus_tag="pgaptmp_001035"
     CDS             complement(4845..5552)
                     /locus_tag="ACHOSC_RS05175"
                     /old_locus_tag="pgaptmp_001035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter transmembrane
                     component T"
                     /protein_id="HGU4547017.1"
                     /translation="MHPFTSLTLWALAACTTLILPAQTILPIYSAATFFCLIALKATQ
                     RRAKYVVWLMFSLGAGLWLVHGGWLPEWLSGTPRSPERWSHAITLWLRILAIVSTSQL
                     WMQYVPVQCFIRALFASRLPPGVAYLFAGPLLVVEQLKRQLAIIHEAQRARGVPLDEG
                     WYQRLRAMPALIIPLTHNALNDLAVRGAALDMRAFRIHNRRTTLWAPADSTLQRVARY
                     TMILLMLTEFGAWIWLR"
     gene            complement(5553..6131)
                     /locus_tag="ACHOSC_RS05180"
                     /old_locus_tag="pgaptmp_001036"
     CDS             complement(5553..6131)
                     /locus_tag="ACHOSC_RS05180"
                     /old_locus_tag="pgaptmp_001036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HGU4547018.1"
                     /translation="MARSHFSSQALVLIVISIAINMIGGQLASMVKLPIFLDSIGTLI
                     SAVLLGPVIGMLTGLLTNLLWGLLTNPIAAAFAPVAMVIGLVAGCLARAGWFRTLPKV
                     VVSGVIITLAVTVVAVPLRTALFGGVTGSGADLFVAWMHSMGQNLVESVAITVIGANL
                     VDKILTAVIVWLLLRQLPIRTTRHFPAMAAVR"
     gene            complement(6154..6585)
                     /locus_tag="ACHOSC_RS05185"
                     /old_locus_tag="pgaptmp_001037"
     CDS             complement(6154..6585)
                     /locus_tag="ACHOSC_RS05185"
                     /old_locus_tag="pgaptmp_001037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PPC domain-containing DNA-binding protein"
                     /protein_id="HGU4547019.1"
                     /translation="MMTLPYPYSSSARFYALRLLPGQEVFSQLHAFAQQHQLHAAWIA
                     GCTGSLTDVALRYAGQEGTTLLNGTFEVISLNGTLEQSGEHLHLCVSDPHGTMLGGHM
                     MPGCTVRTTLELVIGSLEELAFSRQPCALSGYDELHISPVK"
     gene            6957..7976
                     /gene="epd"
                     /locus_tag="ACHOSC_RS05190"
                     /old_locus_tag="pgaptmp_001038"
     CDS             6957..7976
                     /gene="epd"
                     /locus_tag="ACHOSC_RS05190"
                     /old_locus_tag="pgaptmp_001038"
                     /EC_number="1.2.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0048001 - erythrose-4-phosphate
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0042823 - pyridoxal phosphate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="erythrose-4-phosphate dehydrogenase"
                     /protein_id="HGU4547020.1"
                     /translation="MTVRVAINGFGRIGRNVVRALYESGRRAEITVVAINELADAAGM
                     AHLLKYDTSHGRFAWEVRQERDQLFVGDDAIRVLHERSLQSLPWRELGVDVVLDCTGV
                     YGSREHGEAHIAAGAKKVLFSHPGSNDLDATVVYGVNQDQLRAEHRIVSNASCTTNCI
                     IPVIKLLDDAYGIESGTVTTIHSAMHDQQVIDAYHPDLRRTRAASQSIIPVDTKLAAG
                     ITRFFPQFNDRFEAIAVRVPTINVTAIDLSVTVKKPVKANEVNLLLQKAAQGAFHGIV
                     DYTELPLVSVDFNHDPHSAIVDGTQTRVSGAHLIKTLVWCDNEWGFANRMLDTTLAMA
                     TVAFR"
     gene            8026..9189
                     /gene="pgk"
                     /locus_tag="ACHOSC_RS05195"
                     /old_locus_tag="pgaptmp_001039"
     CDS             8026..9189
                     /gene="pgk"
                     /locus_tag="ACHOSC_RS05195"
                     /old_locus_tag="pgaptmp_001039"
                     /EC_number="2.7.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005761248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate kinase"
                     /protein_id="HGU4547021.1"
                     /translation="MSVIKMTDLDLAGKRVFIRADLNVPVKDGKVTSDARIRASLPTI
                     ELALKQGAKVMVTSHLGRPTEGEYNEEFSLLPVVNYLKDKLSNPVRLVKDYLDGVDVA
                     EGELVVLENVRFNKGEKKDDETLSKKYAALCDVFVMDAFGTAHRAQASTHGIGKFADV
                     ACAGPLLAAELDALGKALKEPARPMVAIVGGSKVSTKLTVLDSLSKIADQLIVGGGIA
                     NTFIAAQGHDVGKSLYEADLVDEAKRLLTTCNIPVPSDVRVATEFSETAPATLKSVND
                     VKADEQILDIGDASAQELAEILKNAKTILWNGPVGVFEFPNFRKGTEIVANAIADSEA
                     FSIAGGGDTLAAIDLFGIADKISYISTGGGAFLEFVEGKVLPAVAMLEERAKK"
     gene            9404..10483
                     /gene="fbaA"
                     /locus_tag="ACHOSC_RS05200"
                     /old_locus_tag="pgaptmp_001040"
     CDS             9404..10483
                     /gene="fbaA"
                     /locus_tag="ACHOSC_RS05200"
                     /old_locus_tag="pgaptmp_001040"
                     /EC_number="4.1.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016505616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004332 - fructose-bisphosphate aldolase
                     activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class II fructose-bisphosphate aldolase"
                     /protein_id="HGU4547022.1"
                     /translation="MSKIFDFVKPGVITGDDVQKVFQVAKENNFALPAVNCVGTDSIN
                     AVLETAAKVKAPVIVQFSNGGASFIAGKGVKSDVPQGAAILGAISGAHHVHQMAEHYG
                     VPVILHTDHCAKKLLPWIDGLLDAGEKHFAATGKPLFSSHMIDLSEESLQENIEICSK
                     YLERMSKIGMTLEIELGCTGGEEDGVDNSHMDASALYTQPEDVDYAYTELSKISPRFT
                     IAASFGNVHGVYKPGNVVLTPTILRDSQEYVSKKHNLPHNSLNFVFHGGSGSTAQEIK
                     DSVSYGVVKMNIDTDTQWATWEGVLNYYKANEAYLQGQLGNPKGEDQPNKKYYDPRVW
                     LRAGQTSMIARLEKAFQELNAIDVL"
     gene            complement(10547..10645)
                     /locus_tag="ACHOSC_RS05205"
                     /old_locus_tag="pgaptmp_001041"
                     /pseudo
     CDS             complement(10547..10645)
                     /locus_tag="ACHOSC_RS05205"
                     /old_locus_tag="pgaptmp_001041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518748.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            10841..11701
                     /gene="mscS"
                     /locus_tag="ACHOSC_RS05210"
                     /old_locus_tag="pgaptmp_001042"
     CDS             10841..11701
                     /gene="mscS"
                     /locus_tag="ACHOSC_RS05210"
                     /old_locus_tag="pgaptmp_001042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="small-conductance mechanosensitive channel MscS"
                     /protein_id="HGU4547023.1"
                     /translation="MEDLNVVDSINGAGSWLVANQALLLSYAVNIVAALAIIIVGLII
                     ARMISNAVNRLMISRKIDATVADFLSALVRYGIIAFTLIAALGRVGVQTASVIAVLGA
                     AGLAVGLALQGSLSNLAAGVLLVMFRPFRAGEYVDLGGVAGTVLSVQIFSTTMRTADG
                     KIIVIPNGKIIAGNIINFSREPVRRNEFIIGVAYDSDIDQVKQILTNIIQSEDRILKD
                     REMTVRLNELGASSINFVVRVWSNSGDLQNVYWDVLERIKREFDAAGISFPYPQMDVN
                     FKRVKEDKAA"
     gene            11840..12475
                     /gene="argO"
                     /locus_tag="ACHOSC_RS05215"
                     /old_locus_tag="pgaptmp_001043"
     CDS             11840..12475
                     /gene="argO"
                     /locus_tag="ACHOSC_RS05215"
                     /old_locus_tag="pgaptmp_001043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0061459 - L-arginine transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:1903826 - L-arginine transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine exporter ArgO"
                     /protein_id="HGU4547024.1"
                     /translation="MFSYYFQGLALGAAMILPLGPQNAFVMNQGIRRQYHIMIALLCA
                     ISDLVLICAGIFGGSALLMQSPWLLALVTWGGVVFLLWYGFGAFKTAMSSNIELASAE
                     VLKQGRWKIIATMLAVTWLNPHVYLDTFVVLGSLGGQLDVEPKRWFALGTISASFLWF
                     FGLAILAAWLAPRLRTAKSQRIINLVVGCVMWFIALQLARDGIAHAQALFS"
     gene            12568..13308
                     /gene="yggE"
                     /locus_tag="ACHOSC_RS05220"
                     /old_locus_tag="pgaptmp_001044"
     CDS             12568..13308
                     /gene="yggE"
                     /locus_tag="ACHOSC_RS05220"
                     /old_locus_tag="pgaptmp_001044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxidative stress defense protein"
                     /protein_id="HGU4547025.1"
                     /translation="MKFKVIALAALMGISGMAAQANELPDGPHIVTSGTASVDAVPDI
                     ATLAIEVNVAAKDAATAKKQADERVAQYISFLELNQIAKKDISSANLRTQPDYDYQDG
                     KSILKGYRAVRTVEVTLRQLDKLNSLLDGALKAGLNEIRSVSLGVAQPDAYKDKARKA
                     AIDNAIHQAQELAHGFHRKLGPVYSVRYHVSNYQPSPMVRMMKADAAPVSAQETYEQA
                     AIQFDDQVDVVFQLEPVDQQPAKTPAAQ"
     gene            13475..14371
                     /gene="ygfI"
                     /locus_tag="ACHOSC_RS05225"
                     /old_locus_tag="pgaptmp_001045"
     CDS             13475..14371
                     /gene="ygfI"
                     /locus_tag="ACHOSC_RS05225"
                     /old_locus_tag="pgaptmp_001045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417396.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HGU4547026.1"
                     /translation="MDIFISKKMRNFILLAQTNNIARAAEKIHMTASPFGKSIAALEE
                     QIGYTLFTRKDNNISLNKAGQELYQKLFPVYQRLSAIDNEIHNSGRRSREIVIGIDNT
                     YPTIIFDQLISLGDKYEGVTAQPVEFSENGVIDNLFDRQLDFIISPQHVSARVQELEN
                     LTISELPPLRLGFLVSRRYEERQEQELLQELPWLQMRFQNRANFEAMIDANMRPCGIN
                     PTIIYRPYSFMAKISAVERGHFLTVIPHFAWRLVNPATLKYFDAPHRPMYMQEYLYSI
                     RNHRYTATMLQHIAEDRDGTNH"
     gene            complement(14368..15846)
                     /gene="scpC"
                     /locus_tag="ACHOSC_RS05230"
                     /old_locus_tag="pgaptmp_001046"
     CDS             complement(14368..15846)
                     /gene="scpC"
                     /locus_tag="ACHOSC_RS05230"
                     /old_locus_tag="pgaptmp_001046"
                     /EC_number="2.8.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="propionyl-CoA:succinate CoA transferase"
                     /protein_id="HGU4547027.1"
                     /translation="METQWTRMTADEAAEIIQHNDMVAFSGFTPAGSPKALPTAIARR
                     ANEQHEAKKPYQIRLLTGASISAAADDVLSDADAVSWRAPYQTSSGLRKKINQGAVSF
                     VDLHLSEVAQMVNYGFFGDIDVAVIEASALAPDGRVWLTSGIGNAPTWLLRAKKVIIE
                     LNHYHDPRVAELADIVIPGAPPRRNSVSIFHAMDRVGTRYVQIDPKKIVAVVETNLPD
                     AGNMLDKQNPMCQQIADNVVTFLLQEMAHGRIPPEFLPLQSGVGNINNAVMARLGENP
                     EIPPFMMYSEVLQESVVHLLETGKISGASASSLTISADSLRKIYDNMDYFASRIVLRP
                     QEISNNPEIIRRLGVIALNVGLEFDIYGHANSTHVAGVDLMNGIGGSGDFERNAYLSI
                     FMAPSIAKEGKISTVVPMCSHVDHSEHSVKVIITEQGIADLRGLSPLQRARTIIDNCA
                     HPMYRDYLHRYLENAPGGHIHHDLSHVFDLHRNLIATGSMLG"
     gene            complement(15870..16655)
                     /gene="scpB"
                     /locus_tag="ACHOSC_RS05235"
                     /old_locus_tag="pgaptmp_001047"
     CDS             complement(15870..16655)
                     /gene="scpB"
                     /locus_tag="ACHOSC_RS05235"
                     /old_locus_tag="pgaptmp_001047"
                     /EC_number="7.2.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311816.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl-CoA decarboxylase"
                     /protein_id="HGU4547028.1"
                     /translation="MSYQYVNVVTINKVAVIEFNYGRKLNALSKVFIDDLMQALSDLN
                     RPEIRCIILRAPSGSKVFSAGHDIHELPSGGRDPLSYDDPLRQITRMIQKFPKPIISM
                     VEGSVWGGAFEMIMSSDLIIAASTSTFSMTPVNLGVPYNLVGIHNLTRDAGFHIVKEL
                     IFTASPITAQRALAVGILNHVVEVEELEDFTLQMAHHISEKAPLAIAVIKEELRVLGE
                     AHTMNSDEFERIQGMRRAVYDSEDYQEGMNAFLEKRKPNFVGH"
     gene            complement(16666..17661)
                     /gene="meaB"
                     /locus_tag="ACHOSC_RS05240"
                     /old_locus_tag="pgaptmp_001048"
     CDS             complement(16666..17661)
                     /gene="meaB"
                     /locus_tag="ACHOSC_RS05240"
                     /old_locus_tag="pgaptmp_001048"
                     /EC_number="3.6.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl Co-A mutase-associated GTPase
                     MeaB"
                     /protein_id="HGU4547029.1"
                     /translation="MINEATLAESIRRLRQGERATLAQAMTLVESRHPRHQALSTQLL
                     DAIMPYCGNTLRLGVTGTPGAGKSTFLEAFGMLLIREGLKVAVIAVDPSSPVTGGSIL
                     GDKTRMNDLARAEAAFIRPVPSSGHLGGASQRARELMLLCEAAGYDVVIVETVGVGQS
                     ETEVARMVDCFISLQIAGGGDDLQGIKKGLMEVADLIVINKDDGDNHTNVAIARHMYE
                     SALHILRRKYDEWQPRVLTCSALEKRGIDEIWHAIIDFKTALTASGRLQQVRQQQSVE
                     WLRKQTEEEVLNHLFANEDFDRYYRQTLLAVKNNTLSPRTGLRQLSEFIQTQYFD"
     gene            complement(17654..19798)
                     /gene="scpA"
                     /locus_tag="ACHOSC_RS05245"
                     /old_locus_tag="pgaptmp_001049"
     CDS             complement(17654..19798)
                     /gene="scpA"
                     /locus_tag="ACHOSC_RS05245"
                     /old_locus_tag="pgaptmp_001049"
                     /EC_number="5.4.99.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004494 - methylmalonyl-CoA mutase
                     activity [Evidence IEA];
                     GO_function: GO:0031419 - cobalamin binding [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl-CoA mutase"
                     /protein_id="HGU4547030.1"
                     /translation="MSNVQEWQQLANKELSRREKTVDSLVQQTAEGIAIKPLYTEADL
                     DNLEVTGTLPGLPPYVRGPRATMYTAQPWTIRQYAGFSTAKESNAFYRRNLAAGQKGL
                     SVAFDLATHRGYDSDNPRVAGDVGKAGVAIDTVEDMKVLFDQIPLDKMSVSMTMNGAV
                     LPVLAFYIVAAEEQGVTPDKLTGTIQNDILKEYLCRNTYIYPPKPSMRIIADIIAWCS
                     GNMPRFNTISISGYHMGEAGANCVQQVAFTLADGIEYIKAAISAGLKIDDFAPRLSFF
                     FGIGMDLFMNVAMLRAARYLWSEAVSGFGAQDPKSLALRTHCQTSGWSLTEQDPYNNV
                     IRTTIEALAATLGGTQSLHTNAFDEALGLPTDFSARIARNTQIIIQEESELCRTVDPL
                     AGSYYIESLTDQIVKQARAIIQQIDEAGGMAKAIEAGLPKRMIEEASAREQSLIDQGK
                     RVIVGVNKYKLDHEDETDVLEIDNVMVRNEQIASLERIRATRDDAAVTAALNALTHAA
                     QHNENLLAAAVNAARVRATLGEISDALEVAFDRYLVPSQCVTGVIAQSYHQSEKSASE
                     FDAIVAQTEQFLADNGRRPRILIAKMGQDGHDRGAKVIASAYSDLGFDVDLSPMFSTP
                     EEIARLAVENDVHVVGASSLAAGHKTLIPELVEALKKWGREDICVVAGGVIPPQDYAF
                     LQERGVAAIYGPGTPMLDSVRDVLNLISQHHD"
     gene            complement(20002..20895)
                     /gene="argP"
                     /locus_tag="ACHOSC_RS05250"
                     /old_locus_tag="pgaptmp_001050"
     CDS             complement(20002..20895)
                     /gene="argP"
                     /locus_tag="ACHOSC_RS05250"
                     /old_locus_tag="pgaptmp_001050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator ArgP"
                     /protein_id="HGU4547031.1"
                     /translation="MKRPDYRTLQALDAVIRERGFERAAQKLCITQSAVSQRIKQLEN
                     MFGQPLLVRTVPPRPTEQGQKLLALLRQVELLEEEWLGDEQTGSTPLLLSLAVNADSL
                     ATWLLPALAPVLADSPIRLNLQVEDETRTQERLRRGEVVGAVSIQHQALPSCLVDKLG
                     ALDYLFVSSKPFAEKYFPNGVTRSALLKAPVVAFDHLDDMHQAFLQQNFDLPPGSVPC
                     HIVNSSEAFVQLARQGTTCCMIPHLQIEKELASGELIDLTPGLFQRRMLYWHRFAPES
                     RMMRKVTDALLDYGHKVLRQD"
     gene            21037..21222
                     /locus_tag="ACHOSC_RS05255"
                     /old_locus_tag="pgaptmp_001051"
     CDS             21037..21222
                     /locus_tag="ACHOSC_RS05255"
                     /old_locus_tag="pgaptmp_001051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001328908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4547032.1"
                     /translation="MHFAQRVRALVVLNGVALLPLFAPWSGIPRPLYALFAGRKGMPA
                     IARYFMDELTTRLANGV"
     gene            21278..21937
                     /gene="rpiA"
                     /locus_tag="ACHOSC_RS05260"
                     /old_locus_tag="pgaptmp_001052"
     CDS             21278..21937
                     /gene="rpiA"
                     /locus_tag="ACHOSC_RS05260"
                     /old_locus_tag="pgaptmp_001052"
                     /EC_number="5.3.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004751 - ribose-5-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0009052 - pentose-phosphate shunt,
                     non-oxidative branch [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-5-phosphate isomerase RpiA"
                     /protein_id="HGU4547033.1"
                     /translation="MTQDELKKAVGWAALQYVQPGTIVGVGTGSTAAHFIDALGTMKG
                     QIEGAVSSSDASTEKLKSLGIHVFDLNEVDSLGIYVDGADEINGHMQMIKGGGAALTR
                     EKIIASVAEKFICIADASKQVDILGKFPLPVEVIPMARSAVARQLVKLGGRPEYRQGV
                     VTDNGNVILDVHGMEILDPIAMENAINAIPGVVTVGLFANRGADVALIGTPDGVKTIV
                     K"
     gene            22193..23425
                     /gene="serA"
                     /locus_tag="ACHOSC_RS05265"
                     /old_locus_tag="pgaptmp_001053"
     CDS             22193..23425
                     /gene="serA"
                     /locus_tag="ACHOSC_RS05265"
                     /old_locus_tag="pgaptmp_001053"
                     /EC_number="1.1.1.95"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate dehydrogenase"
                     /protein_id="HGU4547034.1"
                     /translation="MAKVSLEKDKIKFLLVEGVHQKALESLRAAGYTNIEFHKGALDD
                     EQLKESIRDAHFIGLRSRTHLTEDVINAAEKLVAIGCFCIGTNQVDLDAAAKRGIPVF
                     NAPFSNTRSVAELVIGELLLLLRGVPEANAKAHRGVWNKLAAGSFEARGKKLGIIGYG
                     HIGTQLGILAESLGMYVYFYDIENKLPLGNATQVQHLSDLLNMSDVVSLHVPENPSTK
                     NMMGAKEISLMKPGSLLINASRGTVVDIPALCDALASKHLAGAAIDVFPTEPATNSDP
                     FTSPLCEFDNVLLTPHIGGSTQEAQENIGLEVAGKLIKYSDNGSTLSAVNFPEVSLPL
                     HGGRRLMHIHENRPGVLTALNKIFAEQGVNIAAQYLQTSAQMGYVVIDIEADEDVAEK
                     ALQAMKAIPGTIRARLLY"
     gene            23654..23713
                     /gene="ibsC"
                     /locus_tag="ACHOSC_RS05270"
                     /old_locus_tag="pgaptmp_001054"
     CDS             23654..23713
                     /gene="ibsC"
                     /locus_tag="ACHOSC_RS05270"
                     /old_locus_tag="pgaptmp_001054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin IbsC"
                     /protein_id="HGU4547035.1"
                     /translation="MMRLVIILIVLLLISFGAY"
     gene            24046..24105
                     /locus_tag="ACHOSC_RS05275"
                     /old_locus_tag="pgaptmp_001055"
     CDS             24046..24105
                     /locus_tag="ACHOSC_RS05275"
                     /old_locus_tag="pgaptmp_001055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Ibs family toxin"
                     /protein_id="HGU4547036.1"
                     /translation="MMRLAIILIVLLLISFPAY"
     gene            complement(24206..24808)
                     /gene="fau"
                     /locus_tag="ACHOSC_RS05280"
                     /old_locus_tag="pgaptmp_001056"
     CDS             complement(24206..24808)
                     /gene="fau"
                     /locus_tag="ACHOSC_RS05280"
                     /old_locus_tag="pgaptmp_001056"
                     /EC_number="6.3.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311809.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-formyltetrahydrofolate cyclo-ligase"
                     /protein_id="HGU4547037.1"
                     /translation="MTQLPELPLTLSRQEIRKMIRQRRRALTPEQQQEMGQQAATRMM
                     TYPPVVMAHTVAVFLSFDGELDTQPLIEQLWRAGKRVYLPVLHPFSAGNLLFLNYHPQ
                     SELVMNRLKIHEPKLDVRDVLPLSRLDVLITPLVAFDEYGQRLGMGGGFYDRTLQNWQ
                     HYKTQPVGYAHDCQLVEKLPVEEWDIPLPAVVTPSKVWEW"
     gene            complement(24829..25012)
                     /gene="ssrS"
                     /locus_tag="ACHOSC_RS05285"
                     /old_locus_tag="pgaptmp_001057"
     ncRNA           complement(24829..25012)
                     /ncRNA_class="other"
                     /gene="ssrS"
                     /locus_tag="ACHOSC_RS05285"
                     /old_locus_tag="pgaptmp_001057"
                     /product="6S RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00013"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00013"
     gene            complement(25054..25383)
                     /gene="zapA"
                     /locus_tag="ACHOSC_RS05290"
                     /old_locus_tag="pgaptmp_001058"
     CDS             complement(25054..25383)
                     /gene="zapA"
                     /locus_tag="ACHOSC_RS05290"
                     /old_locus_tag="pgaptmp_001058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapA"
                     /protein_id="HGU4547038.1"
                     /translation="MSAQPVDIQIFGRSLRVNCPPDQRDALNQAADDLNQRLQDLKER
                     TRVTNTEQLVFIAALNISYELAQEKAKTRDYAASMEQRIRMLQQTIEQALLEQGRITE
                     KTNQNFE"
     gene            25551..26129
                     /gene="ygfB"
                     /locus_tag="ACHOSC_RS05295"
                     /old_locus_tag="pgaptmp_001059"
     CDS             25551..26129
                     /gene="ygfB"
                     /locus_tag="ACHOSC_RS05295"
                     /old_locus_tag="pgaptmp_001059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708672.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UPF0149 family protein YgfB"
                     /protein_id="HGU4547039.1"
                     /translation="MSIQNEMPGYNEMNQYLNQQGTGLTPAEMHGLISGMICGGNDDS
                     SWLPLLHDLTNEGMAFGHELAQALRKMHSATSDALQDDGFLFQLYLPDGDDVSVFDRA
                     DALAGWVNHFLLGLGVTQPKLDKVTGETGEAIDDLRNIAQLGYDEDEDQEELEMSLEE
                     IIEYVRVAALLCHDTFTHPQPTAPEVQKPTLH"
     gene            26155..27480
                     /gene="pepP"
                     /locus_tag="ACHOSC_RS05300"
                     /old_locus_tag="pgaptmp_001060"
     CDS             26155..27480
                     /gene="pepP"
                     /locus_tag="ACHOSC_RS05300"
                     /old_locus_tag="pgaptmp_001060"
                     /EC_number="3.4.11.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070006 - metalloaminopeptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Xaa-Pro aminopeptidase"
                     /protein_id="HGU4547040.1"
                     /translation="MSEISRQEFQRRRQALVEQMQPGSAALIFAAPEVTRSADSEYPY
                     RQNSDFWYFTGFNEPEAVLVLIKSDDTHNHSVLFNRVRDLTAEIWFGRRLGQDAAPEK
                     LGVDRALAFSEINQQLYQLLNGLDVVYHAQGEYAYADEIVNSALEKLRKGSRQNLTAP
                     ATMIDWRPVVHEMRLFKSPEEIAVLRRAGEITAMAHTRAMEKCRPGMFEYHLEGEIHH
                     EFNRHGARYPSYNTIVGSGENGCILHYTENECEMRDGDLVLIDAGCEYKGYAGDITRT
                     FPVNGKFTQAQREIYDIVLESLETSLRLYRPGTSILEVTGEVVRIMVSGLVKLGILKG
                     DVDELIAQNAHRPFFMHGLSHWLGLDVHDVGVYGQDRSRILEPGMVLTVEPGLYIAPD
                     ADVPEQYRGIGIRIEDDIVITETGNENLTASVVKKPEEIEALMAAARKQ"
     gene            27477..28655
                     /gene="ubiH"
                     /locus_tag="ACHOSC_RS05305"
                     /old_locus_tag="pgaptmp_001061"
                     /gene_synonym="visB"
     CDS             27477..28655
                     /gene="ubiH"
                     /locus_tag="ACHOSC_RS05305"
                     /old_locus_tag="pgaptmp_001061"
                     /gene_synonym="visB"
                     /EC_number="1.14.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008681 - 2-octaprenyl-6-methoxyphenol
                     hydroxylase activity [Evidence IEA];
                     GO_function: GO:0016709 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen, NAD(P)H as one donor, and incorporation
                     of one atom of oxygen [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA];
                     GO_process: GO:0006744 - ubiquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-octaprenyl-6-methoxyphenyl hydroxylase"
                     /protein_id="HGU4547041.1"
                     /translation="MSVIIVGGGMAGATLALAISRLSHGALPVHLIEATAPESHAHPG
                     FDGRAIALAAGTCQQLARIGVWQSLADYATAITTVHVSDRGHAGFVTLAAEDYQLAAL
                     GQVVELHNVGQRLFALLRKAPGVTLHCPDRVANVARTQSHVEVTLESGETLTGRVLVA
                     ADGTHSALATACGVDWQQEPYEQLAVIANVATSVAHEGRAFERFTQHGPLAMLPMSDG
                     RCSLVWCHPLERREEVLSWSDEKFCRELQSAFGWRLGKITHAGKRSAYPLALTRAARA
                     ITHRTVLVGNAAQTLHPIAGQGFNLGMRDVMSLAETLTQAHERGEDIGDYGILCRYQQ
                     RRQSDREATIGVTDSLVHLFANRWAPLVVGRNIGLMTMELFTPARDVLAQRTLGWVAR
                     "
     gene            28678..29880
                     /gene="ubiI"
                     /locus_tag="ACHOSC_RS05310"
                     /old_locus_tag="pgaptmp_001062"
                     /gene_synonym="visC"
     CDS             28678..29880
                     /gene="ubiI"
                     /locus_tag="ACHOSC_RS05310"
                     /old_locus_tag="pgaptmp_001062"
                     /gene_synonym="visC"
                     /EC_number="1.14.13.240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016709 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen, NAD(P)H as one donor, and incorporation
                     of one atom of oxygen [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA];
                     GO_process: GO:0006744 - ubiquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent 2-octaprenylphenol hydroxylase"
                     /protein_id="HGU4547042.1"
                     /translation="MQSVDVAIVGGGMVGLAVACGLQGSGLRVAVLEQRVPEPLAADA
                     PPQLRVSAINAASEKLLTRLGVWQDILSRRASCYHGMEVWDKDSFGHISFDDQSMGYS
                     HLGHIVENSVIHYALWNKAQQSSDITLLAPAELQQVAWGENETFLTLKDGSMLTARLV
                     IGADGANSWLCNKADIPLTFWDYQHHALVATIRTEEPHDAVARQVFHGEGILAFLPLS
                     DPHLCSIVWSLSPEEAQRMQQASEDEFNRALNIAFDNRLGLCKVESARQVFPLTGRYA
                     RQFAAHRLVLVGDAAHTIHPLAGQGVNLGFMDAAELIAELKRLHRQGKDIGQYIYLRR
                     YERSRKHSAALMLAGMQGFRDLFSGTNPAKKLLRDIGLKLADTLPGVKPQLIRQAMGL
                     NDLPEWLR"
     gene            complement(29954..30037)
                     /locus_tag="ACHOSC_RS05315"
                     /old_locus_tag="pgaptmp_001063"
     CDS             complement(29954..30037)
                     /locus_tag="ACHOSC_RS05315"
                     /old_locus_tag="pgaptmp_001063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4547043.1"
                     /translation="MSKNTKSKIRDIRKYNAKTEVKLVYFK"
BASE COUNT         7178 a         7506 c         8112 g         7255 t
ORIGIN      
        1 gacactttat ttaatcaatt aaccgccgaa cataaacact gattacagag gtaaaaatga
       61 aaaccaaagt tgctgctatt tatggcaagc gggatgtccg tctgcgcgaa tttgaactgc
      121 cagaaattac cgataatgaa ctgttagtga gtgtaatttc tgacagcgtc tgtttatcga
      181 cctggaaagc ggcgttactc ggtagtgaac ataaacgcgt acccgacgat ttagaaaatc
      241 atccggtcat taccgggcat gaatgtgccg gggttattgt cgaagtgggt aaaaatctca
      301 ctggcaaata taaaaaaggc cagcgttttg tattgcaacc ggcgatgggg ttaccaagcg
      361 gatattcagc aggctacagc tacgaatatt ttggcggtaa cgccacctat atgattattc
      421 ccgaaatcgc cattaatttg ggctgcgtat taccgtatca cggctcttat tttgctgcgg
      481 cgtcgctggc agagcctatg tgctgcatta ttggtgctta tcatgccaat tatcacacca
      541 cgcaatatgt ttatgagcat cgcatgggcg tcaaacctgg cggcaatatt gcactgctgg
      601 cgtgtgcggg tccgatgggc attggcgcta tcgattacgc cattaacggt ggcatacaac
      661 cgtcacgggt ggtggtggtc gatatcgacg acaaacgcct ggcgcaggtg cagaagctgc
      721 tgccggtgga tctggcggcc agtaaaggca ttgagctggt gtatgtgaat accaaaggga
      781 tgagcgatcc ggtccagacg ctgcgggcgc tgacaggaga tgtcgggttc gatgacattt
      841 ttgtttatgc ggcggtgcct gctgtcgttg agatggctga cgaattactg gcggaagatg
      901 gctgtctgaa cttctttgcc gggccgacgg ataaaaactt caaagtgccg tttaatttct
      961 acaacgtcca ttacaatagc acgcacgtag tcggtacatc cggcggttca acggacgaca
     1021 tgaaagaggc gattgcgtta agcgccacgg ggcagttgca gccgtccttt atggtcacgc
     1081 atatcggtgg gctggatgcg gtgccagata ccgtgctcaa tctgccggat atccctggcg
     1141 gtaaaaaact catttataac ggcgtgacca tgccgctcac tgccattgcc gattttgccg
     1201 aaaaaggcaa aaccgatccg ctgtttaaag agttggcgcg gctggttgag gaaacgcacg
     1261 gcatctggaa tgaacaggcc gagaaatatc tgctggcaca atttggcgtt gatatcgggg
     1321 aggccgcgca atgatgtccc tggcgtggcc attatttcgc gttacggaac aggcagcgtt
     1381 ggctgcctgg ccgcaaaccg gatgtggcga caaaaataaa attgatggcc tggcggtcac
     1441 cgcgatgcgc caggcattaa acgacgtcgc ttttcgtgga cgagtggtta tcggcgaagg
     1501 agaaattgac catgcgccga tgctgtggat tggcgaagag gtgggcaaag gcgatgggcc
     1561 agaagtcgat atcgcggttg acccgattga aggcacgcgg atggtagcga tggggcagag
     1621 caatgcgctg gcggtcatgg ctttcgcccc tcgagatagc ctgttacatg cgcccgatat
     1681 gtatatgaaa aagctggttg ttaatcgact ggctgccggg gcgattgatc tgtctttgcc
     1741 tctggcggac aacttgcgta acgtggcgcg ggcgttaggt aaaccgctgg acaagctgcg
     1801 catggttacg ctggataaac cgcgcctgag tgccgcgatt gaagaagcaa cacagctggg
     1861 cgtgaaggtt ttcgctctgc cggatggcga tgtcgccgcc agcgtgctga cctgctggca
     1921 ggataatcct tacgacgtga tgtacaccat cggcggtgcg ccagaaggcg tgatttctgc
     1981 ctgtgccgtt aaagcattag gtggggatat gcaggccgag ctgattgact tttgtcaggc
     2041 taaaggggat tacacggaaa atcggcagat tgcagagcag gagcgcaagc gttgtaaggc
     2101 aatgggcgtt gacgttaacc gtgtgtactc gctcgatgaa ctggtgaggg ggaacgatat
     2161 cctctttagc gctacgggcg tgacgggcgg cgagttggtg aacggtatcc aacagacggc
     2221 gaatggggtg cggacgcaga cattactgat cggcggcgcg gaccaaacgt gtaatataat
     2281 agactccctg cattgatggt gatctatcgg aagaccaatg gcgacgctga cagaagatga
     2341 tgtgcttgag caactggatg cacaggacaa tttattttca tttatgaaaa ctgcgcattc
     2401 tattttgctc caggggatac gccagtttct gccgtcgctg tttgtcgata acgatgaaga
     2461 gatcgtcgaa tatgcagtga agccgttact cgcccaaagc ggcccgcttg acgatattga
     2521 tgttgcgctg cgtttgattt atgcgctggg gaaaatggat aaatggctgt atgccgatat
     2581 cacgcacttt tcccagtact ggcattacct gaacgaacag gatgaaacgc ccggatttgc
     2641 cgatgacatg acctgggatt ttatcagcaa cgtcaatagc ataacctgca atgcgacgct
     2701 ctatgacgcg ttaaaagcga tgaagttcgc cgatttcgcc gtctggtcag aggcgcgttt
     2761 tagcggaatg gtcaaaacgg cgctgacgct ggcagtaacg accaccttaa aggaattaac
     2821 gccgtgaaaa ttgaattaac ggtgaatggg ctgaacgtgc aagcgcagta ccatgatgaa
     2881 gagatagaac gcgtgcataa accgttgctg cgtatgctgg cggccttaca gacggtaagt
     2941 ccgcagcgac gtacggtggt ttttctttgc gctccgccgg ggacgggtaa atctacctta
     3001 actaccttct gggaatatct cgcgcaacaa gaccctgaat tacccgctat ccagacgctc
     3061 ccgatggatg gttttcacca ttacaatagc tggctggatg cgcatcaatt gcgccccttc
     3121 aaaggcgcac cagagacatt tgacgttgcg aaactggcgg aaaatctgtg ccaggtagtg
     3181 gaaggggatt gtacgtggcc gcagtatgat cgacaaaagc atgatcctgt tgaagatgcg
     3241 ttgcacgtta ccgcaccact cgtcatcgtc gaaggaaact ggttgttgct ggacgacgag
     3301 aagtggtgcc agcttgcgca attttgtgat ttctcgatct ttatcaacgc gccagctact
     3361 gcgttgcggg aacgactggt agggcgcaag ctggcgggag gggtatcgct ggcagacgca
     3421 gaagcttttt atgaccgtac cgacgggccg aatgttcgcc gggggctgga agagagtctg
     3481 cctgcaaatt tgaccttaat gatgaccgct acgggtgaat atcgtctggt ggattaacgt
     3541 tcctgattag accatcttcc agtcgcacca cacgagagaa atgacgccgg gtaaatgcgg
     3601 cgtcatgact gattgctacg acgctcgtgc ctcgctggcc gcacttctcc agccagcttt
     3661 caaacacgct caaccaattt tcgtcaaaat cccgactcgg ctcgtccagc agcaatagtg
     3721 gcggagagag tgcttccagg caggcgacgg cgaccatccg tcgctgcgca ctatgcagat
     3781 ccaacgggtg cgagttggca gtgtctgcta actgacaaca ttgcagggca gcatgggtac
     3841 gttgagtgat ttcatccgcc gggcattttt gcagtttcaa accaaatgcg atctcgtcag
     3901 cgacggtgct gtgaaaaagt tggttttccg cttcctgaaa cagtacgcca acctttgccg
     3961 cgcgctggcg gtttttcagg tttttcatcg cctgttgttg cagcataacc gtaccggctg
     4021 tgggcgtaag aagacctgcc attactcgta acagcgtcga ttttcccgcg ccattgtcgc
     4081 cggtcagcgc caaccattca ccttgtttga gttgcagaga aatgtcgcaa agacaatccg
     4141 ttgccgcgcc aggccagcga taagatatct tatttaacgt taacatagtg gctggagcgc
     4201 tccgttttgc aattgccacg cttgctggca gtagttcagg aaaggtgtgc gatgacgttc
     4261 aaaaagaata atcagcgaac cacgttcaaa cgcccagtgt tgtaagcgct gcaacagcat
     4321 ttcgctggcc tgcggagtca ggcggctaaa ggcttcatca agaattaaga gctttggctg
     4381 catcgcgatg gcgcaggcga tgaccacgcg ctgtgtctct ccaccggaga gtgttgccgg
     4441 gtgacgatgg cgcaacggct ggcactcggt cagagtcagg gcggcatcaa tccgcgccat
     4501 aatctctgct tcagcaagac acagattttc tggcccaaag gcaacttctt cttccacgct
     4561 aaaggtacag ccggagagct gcaaataggg cgattgctgg acgagctgga tggtggcgga
     4621 ttgttcatta agcgggagat gcccaatggg cgtaccgagg agcgtccccg tgcctgttat
     4681 ctctcctggc agaaagtccg gataccagcc cgccatcagc tgtgccagcg tacttttgcc
     4741 actgccgttg tcaccaaaga tggcgaccat ccccggtttg acatagtgaa agtcataaca
     4801 gaacggagga tgcgttgagt gtgtcgggca ataacggaac tgttctaacg taaccatatc
     4861 cacgctccga attctgtcag catcagcaag atcatggtgt aacgcgctac gcgttgtagg
     4921 gtgctatcgg ctggtgccca caacgttgtt cgtcggttgt ggatccgaaa tgcccgcata
     4981 tccagcgccg caccacggac agccagatcg ttaagcgcat tgtgtgttag cggaataatt
     5041 aacgcgggca tcgcacgtaa acgctgatac cagccctcat ccagcggcac gccgcgggca
     5101 cgctgcgctt catgaataat cgctaattgc cgttttaact gctcaacgac caataacggc
     5161 ccggcaaaca gataagcgac gcctggcggc aggcgagaag caaacagagc gcgaataaaa
     5221 cattgcacgg ggacgtactg catccacaac tgtgaggttg aaacaatcgc cagaatccgc
     5281 aaccagagcg taatagcatg cgaccaccgt tctggcgagc gtggcgtgcc gcttagccat
     5341 tctggtagcc agccaccgtg taccagccac agcccggctc ccagcgagaa catcagccag
     5401 acgacatatt ttgcccgccg ctgcgtggcc tttagcgcaa taaggcagaa aaaggtcgcc
     5461 gcgctataga tcggcaaaat ggtctgcgcg ggcaggataa gcgtggtgca ggccgccaat
     5521 gcccacagtg ttaatgaggt aaatggatgc atttagcgca cggcagccat tgccggaaaa
     5581 tggcgcgtgg tgcgaatcgg cagctggcgc agtaaaagcc agacaatcac cgctgtaaga
     5641 attttatcga ccaggttcgc gcctatcacc gtaatcgcca ccgactccac caggttttgc
     5701 cccatcgagt gcatccaggc aacgaacaaa tcggctccgc taccggtgac accgccgaac
     5761 agcgcagtac gcagcggtac agcgacgacc gttaccgcaa gcgtaataat cacgccgctg
     5821 acaacgactt ttggcagggt gcgaaaccaa cccgctcgcg ccaggcaacc ggcgaccagg
     5881 ccgatgacca tcgcgacggg tgcaaatgcg gcggcgatcg ggttggtcag caatccccag
     5941 agtaaattgg tcagtaagcc ggtcagcatt ccgatcaccg gaccaagcag taccgcgcta
     6001 ataagtgtgc cgatggagtc gagaaatata ggtaatttga ccatgctggc cagttgcccg
     6061 ccgatcatat tgatggcgat agatataacg atcaacacca gtgcctgact ggaaaagtgg
     6121 ctacgcgcca taagaaaacc tctttgaaag tagttattta acgggggaaa tatgcaactc
     6181 gtcgtaacca gaaagcgcgc aaggttggcg gctaaacgcc agttcctcaa ggctaccgat
     6241 caccagttca agcgtggtcc gcacggtaca gccgggcatc atatgaccgc caagcatcgt
     6301 gccgtgtgga tcggaaacgc agagatgaag atgctcacca ctctgttcca gcgtgccgtt
     6361 tagtgagatc acttcgaatg taccgttcag gagcgtggtg ccttcttgcc cggcatagcg
     6421 caaggcgacg tcagtcaggc taccggtaca gcctgcaatc caggcggcat gaagttgatg
     6481 ctgctgtgca aaagcgtgta gctgggaaaa aacttcctgc ccggggagga ggcgcagtgc
     6541 gtaaaagcgt gcggaggaag aatatgggta gggcaatgtc atcattggag tactccgaaa
     6601 ctcggacgta ttgcggataa tatcgacgct gcatcagtca tattctgata taagtcaggg
     6661 tttgtgacgg cacaacgaaa agattttgct acgcttattc gtgattctgc aaagtggaat
     6721 aaattccaca ggggcggggc tgaaaacgtg acgtaagtca gataattgct ttctttgact
     6781 ggacaatcat tccttttatt ccacgtttcg cttatcctag ctgaagcgtt tcagtcgatt
     6841 aaatgttcga caattaacca atcagtcgca gtttgcgaca ggtaaggttt ccccggacga
     6901 tttgctggat tactctgtct ggcaaatttg ctcagggaaa accttgcagg agatctatga
     6961 ccgtacgcgt agcgataaat ggcttcggtc gcatcgggcg taatgtggtt cgtgctttgt
     7021 atgaatccgg acgccgggcg gaaattaccg tggtggcaat caacgaactg gcggatgctg
     7081 cgggcatggc gcatttgttg aaatatgaca ccagccatgg ccgttttgca tgggaagtac
     7141 gacaggaacg cgatcaactt tttgttggtg atgacgccat ccgcgtattg catgaacgtt
     7201 cactgcaatc gctcccctgg cgtgaacttg gcgttgatgt agtcctcgac tgcaccggcg
     7261 tatatggctc ccgcgagcat ggcgaagcgc atattgccgc cggggccaaa aaagtgctct
     7321 tttcacatcc tggcagtaac gatctcgacg cgaccgttgt ttacggcgtc aatcaggatc
     7381 aacttcgtgc ggaacaccgc atcgtttcta acgcttcctg taccacgaat tgcataattc
     7441 ccgtcatcaa attgttagat gatgcgtacg gtattgagtc cggcactgtg accacaattc
     7501 actccgccat gcacgatcaa caggttattg atgcatacca tcctgacctg cgtcgcaccc
     7561 gggcagccag ccagtcgatc attccggtcg atactaaact ggccgccggt atcacacgat
     7621 tttttccgca atttaacgat cgctttgaag cgattgcggt acgtgtgcca accataaatg
     7681 tgacggcaat cgatttaagc gtgacggtga agaaacctgt aaaagccaat gaagtcaacc
     7741 tgttgctgca aaaagcagca caaggtgcat ttcatggtat agttgactat acggaattgc
     7801 cgttggtctc tgtagatttt aaccacgatc cgcacagtgc cattgtcgat ggcacccaaa
     7861 cccgggtcag tggcgcacac ctgatcaaaa cgttggtctg gtgcgataac gaatggggct
     7921 ttgctaaccg aatgctcgac acgacgttag ctatggctac tgttgctttc aggtaagacg
     7981 caagcagcgt ctgcaaaact tttagaatca acgagaggat tcaccatgtc tgtaattaag
     8041 atgaccgatc tggatcttgc tgggaaacgt gtatttatcc gtgcggatct gaacgtacca
     8101 gtaaaagacg ggaaagtaac cagcgacgcg cgtatccgtg cttctctgcc gaccatcgaa
     8161 ctggccctga aacaaggcgc aaaagtgatg gtaacttccc acctgggtcg tcctaccgaa
     8221 ggcgagtaca acgaagaatt ctctctgctg ccggttgtta actacctgaa agacaaactg
     8281 tctaacccgg ttcgcctggt taaagattac ctcgacggcg ttgacgttgc tgaaggtgaa
     8341 ctggttgttc tggaaaacgt tcgcttcaac aaaggcgaga agaaagacga cgaaaccctg
     8401 tccaaaaaat acgctgcact gtgtgacgtg ttcgtaatgg acgcattcgg tactgctcac
     8461 cgcgcgcagg cttctactca cggtatcggt aaattcgctg acgttgcgtg cgcaggcccg
     8521 ctgctggcag ctgaactgga cgcgctgggt aaagcactga aagaaccagc tcgcccgatg
     8581 gtggctatcg ttggtggttc taaagtatct accaaactga ccgttctgga ctccttgtct
     8641 aaaatcgctg accagctgat tgttggtggt ggtatcgcta acacctttat cgcggcacaa
     8701 ggccacgatg tgggtaaatc cctgtacgaa gctgacctgg ttgacgaagc taaacgtctg
     8761 ctgaccacct gcaacatccc ggttccgtct gatgttcgcg tagcaaccga gttctctgaa
     8821 actgcaccgg ctaccctgaa atctgttaac gatgtgaaag ctgacgagca gatcctggat
     8881 atcggtgatg cttccgctca ggaactggct gaaatcctga agaatgcgaa aaccattctg
     8941 tggaacggtc cggttggcgt gttcgaattc ccgaacttcc gcaaaggtac tgaaatcgtg
     9001 gctaacgcta tcgcagacag cgaagcgttc tccatcgctg gcggcggcga cactctggca
     9061 gcaatcgacc tgttcggcat tgctgacaaa atctcctaca tctccactgg cggcggcgca
     9121 ttcctcgaat tcgtggaagg taaagtactg cctgcagtag cgatgctcga agagcgcgct
     9181 aagaagtaaa aaatcacagg gcagggaaac ctgcccttgt ttcagcgcgc ttttagagca
     9241 cgcatttttt catactctaa ataattcgag ttgcaggaag gcgacaagag agtgaatccc
     9301 caggagctta cataagtaag tgactggggt gagcgaacgc agacgcagca catgcaactt
     9361 gaagtatgat gagtataagg cccgacgata caggacaaga gacatgtcta agatttttga
     9421 tttcgtaaaa cctggcgtaa tcactggtga tgacgtacag aaagttttcc aggtagcaaa
     9481 agaaaacaac ttcgcactgc cagcagtaaa ctgcgtcggt actgactcca tcaacgccgt
     9541 actggaaact gctgctaaag ttaaagcgcc ggttatcgtt cagttctcca acggtggtgc
     9601 ttcctttatc gctggtaaag gcgtgaaatc tgacgttccg cagggtgctg ctatcctggg
     9661 cgcgatctct ggtgcgcatc acgttcacca gatggctgaa cattatggtg ttccggttat
     9721 cctgcacact gaccactgcg cgaagaaact gctgccgtgg atcgacggtc tgctggacgc
     9781 gggtgaaaaa cacttcgcag ctaccggtaa gccgctgttc tcttctcaca tgatcgacct
     9841 gtctgaagaa tctctgcaag agaacatcga aatctgctct aaatacctgg agcgtatgtc
     9901 caaaatcggc atgactctgg aaatcgaact gggttgcacc ggtggtgaag aagacggcgt
     9961 ggacaacagc cacatggacg cttctgcact gtacacccag ccggaagacg ttgattacgc
    10021 atacaccgag ctgagcaaaa tcagcccgcg tttcaccatc gctgcgtcct tcggtaacgt
    10081 acacggtgtt tacaagccgg gtaacgtggt tctgactccg accatcctgc gtgattctca
    10141 ggaatatgtt tctaagaaac acaacctgcc gcacaacagc ctgaacttcg tattccacgg
    10201 tggttccggt tctactgctc aggaaatcaa agactccgta agctacggcg tagtgaaaat
    10261 gaacatcgat accgataccc aatgggcaac ctgggaaggc gttctgaact actacaaagc
    10321 gaacgaagct tatctgcagg gtcagctggg taacccgaaa ggcgaagatc agccgaacaa
    10381 gaaatactac gatccgcgcg tatggctgcg tgccggtcag acttcgatga tcgctcgtct
    10441 ggaaaaagca ttccaggaac tgaacgcgat cgacgttctg taagtcattc ctttctgctt
    10501 atctcaaggc ccgctctgcg ggtctttttt tcgccaaaag caaaattgcc tgatgcgcta
    10561 cgcttatcag gcctacacgt atcctgcaat atattgaatt tgcaggtttt tgtaggccgg
    10621 ataaggcgtt cacgccgcat ccggcatgac aaagcgcaca ttgatatctg tctgagcccc
    10681 gctctgcggg gattttttcg ccaaaagcaa ggtgattcag atgagaaatc tgtgatctat
    10741 ttggcaaaat tatgctttat tgtttaccct tgtcagactg cccgtcataa ggcggcggag
    10801 tgtatttctc cattttgagt cagttgaaaa ggaatattga atggaagatt tgaatgttgt
    10861 cgatagcata aacggcgcgg gaagctggct ggtagctaac caggcgctgc tgctaagtta
    10921 tgcagtaaac atcgtggcgg cactcgcgat catcatcgtt ggtttgatta tcgcgcggat
    10981 gatttccaac gcggtgaatc gcctgatgat ctcccgtaaa atcgatgcca ctgttgctga
    11041 ttttctttct gcattagtcc gttacggtat tatcgccttt acgctaatcg cagccctggg
    11101 acgcgtgggt gtacaaacag cgtcagtcat tgctgtactc ggtgccgcag gcttagctgt
    11161 tggtctggct ttgcaggggt cactttctaa cctggccgct ggcgtgttac ttgtcatgtt
    11221 ccgcccgttc cgtgccggag aatatgttga cctcggcggc gtagccggta ctgtgctgag
    11281 tgtgcagatt ttctccacca ccatgcgtac tgcagacggt aaaattatcg ttattccgaa
    11341 cggtaaaatt attgccggaa atattattaa cttctcccgc gagccagttc gccgtaacga
    11401 atttattatt ggcgtggcgt atgattccga tatcgatcag gttaagcaga tcctgaccaa
    11461 tattatccag tctgaagatc gcattttgaa agatcgcgaa atgactgtgc gcctgaacga
    11521 acttggtgca tcgtcgatta atttcgtggt ccgcgtctgg agcaacagcg gcgatctgca
    11581 aaacgtgtac tgggatgtgc tggagcgtat taaacgtgaa tttgatgccg ccggtatcag
    11641 cttcccgtac ccgcaaatgg atgtgaactt taagcgggtg aaagaagaca aagctgcgta
    11701 atcaacgctg aggccagata atactctctg gcctctctct tattagttat tctgattgcc
    11761 aattaatatt atcaatttcc gctaataaca atcccgcgat atagtctctg catcagatac
    11821 ttaattcgga atatccaacg tgttttctta ttactttcaa ggtcttgctc ttggggcagc
    11881 tatgattctg ccgctcggcc cacaaaatgc ttttgtgatg aatcagggca ttcgtcgtca
    11941 gtaccacatt atgattgcct tactttgcgc tatcagcgat ttggtcctga tttgcgcggg
    12001 gatttttggt ggtagcgcgt tattgatgca gtcgccgtgg ttgctggcgc tggtcacctg
    12061 gggcggcgta gtcttcttgc tgtggtatgg ttttggcgct tttaaaacag cgatgagtag
    12121 taatattgag ttagctagtg ccgaagtcct gaagcaaggc agatggaaaa ttatcgccac
    12181 catgttggca gtgacctggc tgaatccgca tgtttacctg gatacttttg ttgtactggg
    12241 cagccttggc gggcagcttg acgtcgaacc aaaacgttgg tttgcgctcg ggacaattag
    12301 cgcctctttc ctgtggttct ttggtctggc tattctcgca gcctggctgg caccgcgcct
    12361 gcgcacggca aaatcacagc gcattatcaa tctggtagtg ggatgtgtca tgtggtttat
    12421 agccttgcag ctggcgagag acggtattgc tcatgcacaa gccttgttca gttaggcgct
    12481 atctgatgga aaaataaaac agaggcgcta agcttgcctc cagaggtcct gaattttggg
    12541 caagtcaatt aacacggaga gactaacgtg aagttcaaag ttatcgccct ggcggcatta
    12601 atgggtatta gcgggatggc agcgcaggct aacgaattgc cggatggacc gcatattgtc
    12661 acctccggta cggcaagtgt ggatgcggtg ccagacattg ccactcttgc gattgaagtt
    12721 aacgtggccg cgaaggatgc cgctacagcc aagaaacagg cagatgagcg cgtcgcacaa
    12781 tacatttcct tccttgaact caatcagatc gcgaaaaaag atatcagctc agcgaattta
    12841 cgcacccagc cagattatga ttatcaggat ggtaaaagta tcctgaaagg ctaccgcgct
    12901 gtgagaacgg tggaagtcac gctccgtcag ttagacaaac tgaattcctt gctggatggc
    12961 gcgctgaagg cgggtcttaa cgaaattcgt tctgtgtcgc tgggcgtggc gcagccggat
    13021 gcctataaag acaaagcgcg taaggcagcg attgataacg cgattcatca ggcgcaggaa
    13081 ctggcgcacg gctttcatcg taaactgggg ccggtatata gcgtgcgcta ccatgtttcc
    13141 aactatcagc ccagcccaat ggtgcggatg atgaaagccg atgccgcgcc ggtgtctgcc
    13201 caggaaactt acgagcaggc cgctattcag tttgatgatc aggtcgatgt ggtcttccag
    13261 ttagaacctg tggatcaaca acccgctaaa acacctgcag cacaataata cttgtagccc
    13321 gacgtattcg tcgggctaat gcctttaccc gatattgcct cctttttctg taattttctg
    13381 taaaaccatg acgtaatcac ctatctttgc tcgcggtttc atcaaggata ggctgactga
    13441 aacggttaag cggacaagga tgcggggagg ggggatggac atttttattt cgaaaaagat
    13501 gcgcaatttc attttattag cgcaaaccaa taatattgcc cgggcggcag aaaaaatcca
    13561 tatgacggct tcgccatttg gtaaaagcat tgccgcgctg gaagaacaaa ttggctatac
    13621 gctatttacc cgcaaagata ataatatcag cctcaataaa gccggtcagg aactgtatca
    13681 aaaactgttc ccggtttatc aacgactttc tgcgattgat aatgaaatcc ataactcagg
    13741 gcgtcgttca cgagaaattg tgattggcat agataatact tatccgacaa ttattttcga
    13801 tcagttgatt agtctcggag ataagtatga aggcgtgacc gcccagccag ttgagttcag
    13861 cgaaaacggt gttatcgata atctttttga ccgtcagtta gattttatta tctccccaca
    13921 gcatgtatct gcacgagttc aggaactgga aaacctgact atcagtgaat taccaccgct
    13981 gcgattgggc tttctcgttt ctcgccgtta tgaagagcgt caagagcaag agctgcttca
    14041 ggagctgccc tggctacaaa tgcgctttca aaaccgggct aacttcgaag caatgattga
    14101 tgcgaatatg cgaccttgtg ggatcaatcc caccattatt tatcgtccct acagttttat
    14161 ggccaaaatc agcgccgtgg agcgcggaca ttttctgacg gtgatcccgc attttgcctg
    14221 gcgactggtg aatccggcaa cgctgaaata ttttgatgcg ccgcacaggc cgatgtatat
    14281 gcaggaatat ctttattcaa tcagaaatca tcgttatacc gccacgatgc ttcagcatat
    14341 tgctgaagat cgtgacggga caaaccatta acccagcatc gagccggttg caattaaatt
    14401 acggtgtaag tcgaagacgt ggctaagatc gtggtgaata tgtccgccgg gcgcattttc
    14461 cagatagcga tgcagataat cccgatacat aggatgtgca caattatcaa tgatagtgcg
    14521 ggcgcgttga agcggggaaa gaccgcgcag atcggcgatc ccttgttcgg tgatgatcac
    14581 tttgacgctg tgttcgctgt gatcaacatg gctgcacatt ggcacgacgg ttgagatctt
    14641 gccttcttta gcaatcgacg gagccataaa gatcgacaga tacgcgttgc gttcaaaatc
    14701 accgctgccg ccgatgccgt tcatcagatc gaccccggct acgtgcgttg agttggcatg
    14761 cccgtaaata tcaaactcca ggccgacgtt cagagcgatg acgcccagac gacggatgat
    14821 ttccgggtta ttggaaatct cctgcggacg caacacaatg cggctggcaa agtaatccat
    14881 attgtcgtaa atcttgcgca gggaatcggc cgagattgtc aggctggagg cgctggcccc
    14941 gctgattttg ccggtttcca gtaaatgcac caccgattcc tgtagcactt ccgaatacat
    15001 catgaacgga ggaatttccg ggttttcccc cagacgcgcc attaccgcat tattgatatt
    15061 gcccacgcca ctttgcagcg gcagaaattc cggcggaata cgcccatgcg ccatttcctg
    15121 caataagaac gtgaccacgt tatcggcaat ctgctggcac atgggatttt gcttatccag
    15181 catattaccg gcgtcgggca agttggtttc cacgacggcg acaatctttt tcggatcgat
    15241 ttgcacatag cgggtaccga cgcgatccat tgcatggaag atcgacacgc tattgcgccg
    15301 cggtggcgcg ccaggaatca caatatctgc cagttctgca acgcgcggat cgtgatagtg
    15361 attaagttca atgatcactt tcttcgctcg cagcagccag gtcggcgcat taccgatccc
    15421 gctggttaac cagactcgac catccggtgc cagtgccgat gcttcaatga cggcaacatc
    15481 aatgtcgccg aagaaaccgt aattgaccat ttgcgccact tcgctcaaat gcaggtcaac
    15541 gaaactcacc gcgccctgat tgatcttttt acgtaaaccg gacgatgttt gatatggcgc
    15601 acgccaggaa acagcatcgg cgtcagaaag tacatcgtca gcggcggcgc tgattgacgc
    15661 ccccgtcagc aggcgaattt gatacggctt tttggcctca tgctgttcgt tagctctgcg
    15721 ggcaatcgcg gtgggtaggg ctttcggcga acccgccggg gtaaagccgc taaatgccac
    15781 catgtcgtta tgctggataa tttctgccgc ttcatcggcg gtcatccttg tccactgagt
    15841 ttccattttt actccttcgt tcgcagggat taatgaccaa cgaaattagg tttacgtttt
    15901 tcgaggaaag cgttcattcc ttcctggtaa tcttcgctgt cataaaccgc gcgtcgcatc
    15961 ccctgaatac gttcaaattc atcggagttc atggtgtgcg cttcgcccag tacacgcagc
    16021 tcttctttga taacagcaat ggctaacggc gctttctcag agatgtggtg cgccatttgt
    16081 aaggtgaaat cttccagttc ttccacttcc acaacatggt tgaggatgcc gacagccaac
    16141 gcgcgctggg cggtgattgg cgaagcggta aaaatcagtt ctttgacaat gtggaagccc
    16201 gcgtcgcggg tcaggttgtg aatgccgacc aggttatacg ggacgccgag gtttacaggc
    16261 gtcattgaga aggttgaggt actggcggcg ataatcagat cggaactcat gatcatttca
    16321 aatgcgccac cccaaacact accttccacc atcgaaatga tcggtttcgg gaatttttgg
    16381 atcatgcggg tgatttgacg caatggatca tcataggaga gcggatcgcg accgccagac
    16441 ggcagttcgt gaatatcgtg gcctgcggag aagactttgg atccactcgg cgcgcgcaaa
    16501 atgatacagc gaatttccgg ccggttgaga tcgcttaatg cctgcataag atcatcaata
    16561 aagactttac ttaaggcatt aagtttgcgg ccatagttaa actcaatgac cgccactttg
    16621 ttgatagtga caacgttaac atactgataa gacataaaaa ttcctttaat caaaatattg
    16681 cgtctggata aattcactga gctgccgcag gccggtgcgc ggtgagagcg tattgttttt
    16741 gaccgctaaa agcgtctggc ggtaatagcg atcgaaatct tcattcgcga acaggtgatt
    16801 cagtacttct tcttcggtct gcttacgcag ccattccacc gattgttgtt gccgcacttg
    16861 ttgtaaacga ccactggcag ttagcgcggt tttgaagtcg atgatggcgt gccagatctc
    16921 atcgattcca cgtttttcca gtgcgctaca agtcagaacc cgtggctgcc attcgtcgta
    16981 tttacgtcgc agaatatgca gggcactctc gtacatatgc cgggcaatgg cgacattggt
    17041 atggttatcg ccatcgtctt tgttgataac gatcagatca gccacttcca tcagcccttt
    17101 tttaatgccc tgcagatcat cgccgccacc ggcaatttgc aacgagataa aacagtccac
    17161 catgcgggcg acttctgttt ccgactgccc gacgccaacc gtttcgacaa tcactacgtc
    17221 ataacccgct gcttcgcata acagcattaa ttcccgcgct cgctgactgg caccgcccag
    17281 atgaccggag gatggtaccg ggcgaataaa cgccgcttcg gcacgcgcca ggtcattcat
    17341 gcgggtttta tccccgagaa tgctaccgcc agtgaccggg ctgctgggat cgaccgcaat
    17401 aaccgcgacc tttaatccct ctcgaatcaa caacatgcca aaggcctcaa gaaaggtact
    17461 tttccccgcg ccgggggtgc cggtaacgcc cagtcgcagg gtgttaccgc agtacggcat
    17521 aatggcatca agcagctgcg tacttagtgc ctgatgacgc gggtgacggc tttccaccag
    17581 cgtcatggcc tgggcgagtg tggcacgctc accctgacgt aagcggcgaa tactttctgc
    17641 cagcgtggct tcattaatca tgatgctggc ttatcagatt cagtacgtcg cgcacactgt
    17701 cgagcatagg tgtacctgga ccataaatcg ccgccacgcc gcgctcttgc aggaaggcgt
    17761 aatcctgcgg cggaatgacg ccacccgcga ccacgcagat atcttcgcgt ccccattttt
    17821 tcagcgcttc gaccagttcc gggatcagcg ttttatgacc ggcagccagt gaggatgcgc
    17881 ccactacgtg aacgtcgttt tctacggcca ggcgggcgat ctcttcaggt gtagagaaca
    17941 tcgggcttaa atctacgtcg aaaccgagat cggaataggc gctggcgatc actttcgcgc
    18001 cgcgatcgtg tccatcctgg cccatcttag cgatcagaat gcgcgggcga cgaccattgt
    18061 cggcaaggaa ctgctccgtt tgcgcaacaa tggcatcgaa ctcggaggcc gatttctcag
    18121 actgatgata gctttgcgca atcacgccgg taacacactg gcttggcacc agataacggt
    18181 cgaaagcgac ttccagcgca tcggaaattt cacccagggt ggcgcgaacg cgagcggcat
    18241 taacagcggc agccagcagg ttttcgttat gctgtgcggc gtgagtcagg gcgttcaacg
    18301 cggcggttac ggcggcatca tcacgggtgg cgcgaatgcg ttccagcgaa gcaatttgct
    18361 cgttacgcac catcacgttg tcgatctcaa gtacatcggt ttcgtcttcg tgatccagtt
    18421 tgtacttgtt gacaccaacg atgacacgct tgccctggtc gatcagcgac tgttcgcgcg
    18481 ctgaggcctc ttcgatcatt cgttttggca gacctgcttc gatcgctttc gccatgccac
    18541 cggcttcgtc gatctgttgg ataatagctc tggcttgttt gacgatttga tcggtcagcg
    18601 actcaatgta ataggatccg gccagtggat cgacggtgcg gcagagttct gattcttcct
    18661 ggatgatgat ctgggtgttg cgggcaatgc gtgctgagaa atcggtaggc aaaccaagcg
    18721 cttcgtcaaa ggcgttggta tgcagtgact gagtaccgcc cagcgtcgca gccagtgctt
    18781 caatggtggt gcggataacg ttgttatacg gatcctgttc agtcaggctc cagcctgagg
    18841 tctggcagtg ggtacgcagc gccagtgatt tcggatcctg tgcgccaaat ccactgaccg
    18901 cttcgctcca taaataacgt gccgcacgca acatggcgac gttcataaac agatccatgc
    18961 cgataccgaa gaagaacgac aggcgaggag cgaagtcatc aattttcagt ccggcagaga
    19021 ttgctgcttt gatgtactca atcccatcag cgagcgtaaa tgctacctgc tgcacgcagt
    19081 tggcacccgc ttcccccatg tggtaaccgc tgatactgat ggtattaaat cgcggcatgt
    19141 tgccggaaca ccaggcgatg atgtcggcga taatgcgcat tgacggtttt ggtgggtaaa
    19201 tataggtgtt gcggcagagg tactctttga ggatatcgtt ctgaatggtg ccggtcagtt
    19261 tatcaggtgt aacaccttgc tcttctgcgg cgacgatata aaacgccagt actggtagca
    19321 ctgcgccatt catggtcatc gaaaccgaca ttttatccag cgggatctgg tcgaacagga
    19381 ctttcatatc ttccacggtg tcgatagcga cgcccgcttt gccgacgtcg cccgccacgc
    19441 gcgggttatc ggagtcgtag ccacggtggg tggcaaggtc aaacgcaacg gaaagacctt
    19501 tttgcccggc ggccaggtta cggcgataaa aagcgttgga ctcttttgct gttgaaaaac
    19561 cagcatactg acggatggtc cacggttggg cggtatacat agtggcacgc gggccacgaa
    19621 cgtagggcgg caaaccagga agggtacctg tcacctccag attatcgaga tcggcttcgg
    19681 tatacagcgg cttgatggcg atcccttccg cggtttgctg aaccagcgag tcgacagttt
    19741 tctcccgacg gctcaattcc ttgttggcaa gctgttgcca ctcctgcacg ttagacatta
    19801 tttgctccgt aaaacgcaat caaaatgagg gtatttgcgc ctacggtgag ctttttacgt
    19861 gggtttgtcg ccagcaaaaa aaatgcattc agacgtcgtt ttttgccgcc tgagatttgt
    19921 ctattttgtg atgtgattca aggaatacaa aaagaagtga ggacaacgcc tgatatgtgc
    19981 tatcaggcat tatttgatgg attaatcctg acgaaggact ttgtgaccat aatcgagtaa
    20041 cgcatcagtg actttacgca tcatgcggct ttcaggagca aagcggtgcc agtagagcat
    20101 ccgtcgttga aatagcccag gcgttaagtc aatcagttca ccgctggcca gctctttctc
    20161 gatttgcagg tgcgggatca tacagcaggt ggtgccctgg cgagcaagtt gtacgaacgc
    20221 ttctgaagaa ttaacgatat ggcagggcac gctgcctgga ggcagatcga agttttgctg
    20281 caaaaaggcc tggtgcatat cgtcaagatg gtcaaacgcg accactggcg ctttcagtaa
    20341 tgccgaacgc gttacgccgt tagggaaata tttttcggca aagggttttg agctgacgaa
    20401 cagatagtcg agcgcaccaa gtttatcgac aagacaactc ggcagcgcct gatgttgaat
    20461 actcaccgcg ccgaccactt cgccgcggcg cagacgttcc tgagtgcggg tttcatcttc
    20521 tacctgcaag ttgaggcgga taggcgaatc agccaacaca ggagccagtg caggaagcaa
    20581 ccacgtcgcc agactgtcgg cgttgaccgc cagtgaaagc agcagcggag tcgaaccggt
    20641 ttgttcatcg cccagccact cttcttccag caactccacc tggcgcagca gtgccagcag
    20701 tttttgccct tgttccgtcg ggcgcggcgg tacggtacgc accaacagcg gctgcccgaa
    20761 catattttcc agttgcttaa tgcgctgtga gacggctgat tgtgtaatgc acagcttttg
    20821 tgccgcgcgc tcaaatcctc gttcacgtat caccgcatcc agtgcctgta atgttctgta
    20881 gtccgggcgt ttcattgctg ttgtctgctc ctggtaaatg ctttttcctg cactatgaca
    20941 caattttgtg tcaggttgca tataccgaag ccgtcggcgg gtaacatttg tattcgtggt
    21001 taaacgcgca gcttaaaaga tcagggattt gcgctaatgc atttcgctca gcgagttcgc
    21061 gccctggttg tgttgaatgg cgtggcgtta ttgcctctgt tcgcaccgtg gagcggcata
    21121 cccagaccgt tgtatgcttt atttgcgggg cgaaagggga tgcccgccat tgcgcgatat
    21181 tttatggatg agttaaccac gcggcttgcc aacggggtct gaatcgcttt ttttgtatat
    21241 aatgcgtgtg aaatttcata ccacaggcga aacgatcatg acgcaggatg aattgaaaaa
    21301 agcagtagga tgggcggcac ttcagtatgt tcagcccggc accattgttg gtgtaggtac
    21361 aggttccacc gccgcacact ttattgacgc gctcggtaca atgaaaggcc agattgaagg
    21421 ggccgtttcc agttcagatg cttccactga aaaactgaaa agcctcggca ttcacgtttt
    21481 tgatctcaac gaagtcgaca gccttggcat ctacgttgat ggcgcagatg aaatcaacgg
    21541 ccacatgcaa atgatcaaag gcggcggcgc ggcgctgacc cgtgaaaaaa tcattgcttc
    21601 ggttgcagaa aaatttatct gtattgcaga cgcttccaag caggttgata ttctgggtaa
    21661 attcccgctg ccagtagaag ttatcccgat ggcacgtagt gcagtggcgc gtcagctggt
    21721 gaaactgggc ggtcgtccgg aataccgtca gggcgtggtg accgataatg gcaacgtgat
    21781 cctcgacgtc cacggcatgg aaatccttga cccgatagcg atggaaaacg ccataaatgc
    21841 gattcctggc gtggtgactg ttggcttgtt tgctaaccgt ggcgcggacg ttgcgctgat
    21901 tggcacacct gacggtgtca aaaccattgt gaaatgatct gacgggggaa cctcccccgt
    21961 taaaaaaatt ctcttcatta aatttggtga catgtgtcac gcttttacca ggcaattgtc
    22021 gattgctcta aataaatcct ctaaaccagc atattcatcc aagaattacc tttgcgtgat
    22081 atttcctcaa catcgcgacg caaacgttca tattgccgca atattatttt ttgatatgtt
    22141 gaaaggcgga tgcaaatccg cacacaacat ttcaaaagac aggattgggt aaatggcaaa
    22201 ggtatcgctg gagaaagaca agattaagtt tctgctggta gaaggcgtgc accaaaaggc
    22261 gctggaaagc cttcgtgcag ctggttacac caacatcgaa tttcacaaag gcgcgctgga
    22321 tgatgaacaa ttaaaagaat ccatccgcga tgcccacttc atcggcctgc gatcccgtac
    22381 ccatctgact gaagacgtga tcaacgccgc agaaaaactg gtcgctattg gctgtttctg
    22441 tatcggaaca aaccaggttg atctggatgc ggcggcaaag cgcgggatcc cggtatttaa
    22501 cgcaccgttc tcaaatacgc gctctgttgc ggagctggtg attggcgaac tgctgctgct
    22561 attgcgcggc gtgccggaag ccaatgctaa agcgcaccgt ggcgtgtgga acaaactggc
    22621 ggcgggttct tttgaagcgc gcggcaaaaa gctgggtatc atcggctacg gtcatattgg
    22681 tacgcaattg ggcattctgg ctgaatcgct gggaatgtat gtttactttt atgatattga
    22741 aaacaaactg ccgctgggca acgccactca ggtacagcat ctttctgacc tgctgaatat
    22801 gagcgatgtg gtgagtctgc atgtaccaga gaatccgtcc accaaaaata tgatgggcgc
    22861 gaaagagatt tcgctaatga agcccggctc gctgctgatt aatgcctcgc gcggtactgt
    22921 ggtggatatt ccggcgctgt gtgatgcgct ggcgagcaaa catctggcgg gggcggcaat
    22981 cgacgtattc ccgacggaac cggcgaccaa tagcgatcca tttacctctc cactgtgtga
    23041 attcgacaac gtccttctga cgccacacat tggcggttcg actcaggaag cgcaggagaa
    23101 tatcggcctg gaagttgcgg gtaaattgat caagtattct gacaatggct caacgctctc
    23161 tgcggtgaac ttcccggaag tctcgctgcc actgcatggt ggtcgtcgtc tgatgcacat
    23221 ccacgaaaac cgtccgggcg tgctaactgc gctgaacaaa atcttcgccg agcagggcgt
    23281 caacatcgcc gcgcaatatc tacaaacttc cgcccagatg ggttatgtag ttattgatat
    23341 tgaagctgac gaagacgttg ccgaaaaagc gctgcaggca atgaaagcta ttccgggtac
    23401 cattcgcgcc cgtctgctgt actaattccc cttctctgaa aattaacggg caggtcactg
    23461 acttgcccgt ttttttatcc cttctccaca ccgttgccgc gttgcgcatt ctactttgcg
    23521 agtctgctcc agaaaataaa tcaaagtcag atcaatgcgt tgtgtttatt taatggggat
    23581 ttacgatggc gggacagcat ggggctgtaa cggtaaagcc ctcaccgaag cgagggctta
    23641 aaggagaagg gttatgatgc gacttgtcat catactgatt gtactgttac tgataagttt
    23701 cggagcttat taacagtcag tctcagggga ggagtcatcc tcccttaccc ttactcacta
    23761 aattaggtca aagaatcaac gatgtcaatc agggcgatgc ggggtgcatc gccctgaacc
    23821 gaactttttg atagccactt catcgcccac gtttactgcc gtaatagatc ctttttccca
    23881 aaaattatct ctgttttgca tggggttttt cgagcacgct cgaagaaaag atttaaaggt
    23941 agatcaatgc gttgtggtta tttaaagaga gcttacgata gctgggcagc atggggctgt
    24001 aacggtaaag ccctcaccga agcgagggct tgaaggagaa gggttatgat gcgacttgcc
    24061 atcatactga ttgtactgtt actcataagt ttcccagctt attaacagtc agtcacaggg
    24121 gaggagcaat cctcccttac ccttactcac taaattaggt taaagaatca acgatgtcaa
    24181 tcagggcgat gcaggtgtat cgcccttacc actcccagac tttcgacggt gtaaccaccg
    24241 caggaagagg gatatcccac tcttcaacgg ggagtttttc caccaactga caatcatgcg
    24301 cataacccac cggttgcgtt ttatagtgct gccagttttg taaggtccga tcataaaaac
    24361 caccgcccat tcccaggcgc tgaccgtact catcaaaggc gaccagcggt gtgatcagca
    24421 cgtctaatcg ggaaaggggt agcacgtcac gcacatccag ttttggctca tggatcttca
    24481 acctgttcat caccagttcg ctttgcggat ggtaattcag gaacagcaaa ttaccggcac
    24541 taaagggatg caaaactgga agatatacgc gcttaccggc gcgccagagt tgttctatta
    24601 gcggctgggt gtcgagttca ccatcaaaag agaggaatac agcgaccgta tgcgccatca
    24661 ccaccggggg ataagtcatc atccgggtag cggcttgttg acccatttcc tgctgttgtt
    24721 ccggcgttaa cgcacgacga cgttgccgaa tcattttgcg gatttcttgt cgggataatg
    24781 tcagtgggag ttctggtagt tgcgtcatgg ctggtgccag ataagaaggg aatctccgag
    24841 atgccgccgc aggctgtaac ccttgaaccc ttggttcaag gtgaatgtgt cgtcgcagtt
    24901 ttaaggcttc tcggacggac cgagcatgct caccaaccgc ggagcgccac attcttgtgg
    24961 tatgaaatat cggctcaggg gactggcccg cttgcgaaca tctcagagaa attttgtctt
    25021 cacggttact ctaccacagt aaaccgaaaa gtgtcattca aagttttggt tagttttttc
    25081 ggtgatgcga ccttgttcaa gtaacgcttg ttctatggtc tgctgcagca tccgaatacg
    25141 ctgttccata cttgccgcgt agtcacgagt cttcgctttt tcttgcgcta actcatagct
    25201 gatattcaat gcggcaatga agaccaactg ttcagtattt gtgactctag tgcgttcttt
    25261 cagatcttgc aaccgttggt tcagatcgtc cgctgcctga ttcaacgcat ccctttggtc
    25321 aggcgggcag ttcacacgca gtgaacggcc aaaaatttgg atatcgacgg gttgtgcaga
    25381 catgccacct tcctgctgat tgactgcgct gccttcgtct ttagacccat ggtctgcgaa
    25441 ggggcgacac tatagctacc ctgatgagaa gagacaagcc cttttctggt ccaccagggc
    25501 ccaaagtggt agcatatcat gaatattcct ccctttgacg acgaatgctt atgtctatac
    25561 agaacgaaat gcctggttac aacgaaatga accagtatct gaaccaacaa gggacgggtc
    25621 tgacccctgc tgagatgcat ggtttaatca gtgggatgat atgtggcggt aacgatgaca
    25681 gctcatggct accgctactt cacgacctga cgaacgaagg catggctttc ggtcatgagc
    25741 tggcacaggc actgcgtaaa atgcactctg ccaccagcga tgccctgcag gatgacggct
    25801 tcctttttca gctttatctg cctgatggcg atgatgtcag cgttttcgat cgggctgatg
    25861 cattggcagg ttgggtcaat cacttcctgc ttggtcttgg cgttacgcaa ccgaagctgg
    25921 ataaagtgac cggcgaaacc ggtgaagcta tcgacgatct gcgtaacatt gcgcaactgg
    25981 gttacgacga agacgaagat caggaagagc ttgaaatgtc gcttgaagag atcatcgaat
    26041 acgttcgtgt tgccgcgctg ttatgccacg acacctttac tcatccgcaa ccgaccgcgc
    26101 cagaagtaca aaaaccgact ctacactaaa aacaaaaaac gtaaggagag tgttatgagt
    26161 gagatatccc ggcaagagtt tcagcgtcgc cgtcaggccc tggtggagca aatgcaaccc
    26221 ggcagcgccg cgctgatttt tgctgcacca gaagtaacac gtagcgccga cagcgaatac
    26281 ccctatcgtc agaacagtga cttctggtac ttcaccggct ttaacgaacc ggaagcggtg
    26341 ctggtgctga ttaaaagcga tgacactcat aaccacagcg ttctgtttaa ccgcgttcgc
    26401 gacctgacgg cggagatctg gtttggccgt cgcttaggcc aggatgccgc gccagagaaa
    26461 ctgggcgttg accgcgcact ggcattcagc gaaatcaatc agcaacttta tcaactactt
    26521 aacggcctgg atgtggttta ccatgcccag ggcgaatatg catatgctga tgaaatcgtg
    26581 aacagtgcgc tggaaaaact gcgtaaaggt tcgcggcaaa atctcaccgc accggcaacg
    26641 atgatcgact ggcgtcctgt tgttcatgaa atgcgcctgt tcaaatcgcc agaagagatt
    26701 gccgtactcc gccgcgcggg agaaatcacc gccatggcac atacccgggc gatggaaaaa
    26761 tgccgtccgg gaatgttcga gtaccatctg gaaggcgaaa ttcaccacga atttaaccgc
    26821 cacggtgcgc gctatccgtc ctataacacc attgtcggca gcggtgaaaa cggctgcatt
    26881 ctgcactaca ccgaaaacga gtgtgaaatg cgcgacggcg acctggtgtt gattgacgcg
    26941 ggttgcgaat acaaaggtta cgctggcgat attacccgca ccttcccggt caacggcaaa
    27001 ttcactcagg cccagcgtga aatctacgac attgtgctgg agtctctcga aaccagcttg
    27061 cgcctgtatc gtccgggaac ttccattctg gaagtcactg gtgaagtggt gcgcatcatg
    27121 gttagcggcc tggtaaaact cggcattctg aaaggtgatg ttgatgaact gatcgctcag
    27181 aacgcccatc gtcctttctt tatgcatggc cttagccact ggttagggct agatgtccat
    27241 gacgttggcg tttatggtca ggatcgctcg cgcattctgg aaccggggat ggtactgacc
    27301 gtagagcctg gtctgtatat cgcgccggat gcggatgtgc cagaacaata tcgcggtatc
    27361 ggcattcgta ttgaagacga cattgtgatt accgaaaccg gcaacgaaaa cctcaccgcc
    27421 agcgtggtga aaaagccgga agaaatcgaa gcgttgatgg cagctgcgag aaagcaatga
    27481 gcgtaatcat cgtcggtggc ggcatggcgg gcgcgacgct ggcgctggct atttcccggt
    27541 taagtcacgg ggcgctgccg gtacatttga ttgaagcgac cgcgccagag tcacatgctc
    27601 atccgggctt tgatggacga gctattgcgc tggctgcggg gacctgtcag caactggcgc
    27661 gcatcggcgt ctggcaatct ctggcggatt acgcaaccgc catcactacc gtgcatgtca
    27721 gcgatcgtgg tcacgctgga tttgtcaccc tcgccgcaga agattaccaa ctggcggcgc
    27781 tgggacaggt tgtcgaattg cacaatgtcg ggcaacggct gtttgcattg ctgcgtaaag
    27841 cacctggcgt aacgctgcat tgccctgatc gcgtggctaa cgttgcccgt actcagagtc
    27901 acgttgaagt gacgctggag agtggcgaga cgctgacggg ccgcgtgctg gtagcagctg
    27961 atggcaccca ttcagcgtta gccaccgcgt gcggcgttga ctggcagcag gagccttacg
    28021 aacaactggc cgtgattgcc aacgttgcga cttccgttgc gcacgaaggc cgcgcttttg
    28081 aacgcttcac gcaacatggc ccgctggcga tgttgccgat gtctgacgga cgctgttcgc
    28141 tggtctggtg tcatccactg gaacggcgcg aagaggtgct gtcgtggagc gacgagaagt
    28201 tttgccgtga actccagtcg gcctttggct ggcgacttgg aaaaattacc cacgccggta
    28261 aacgcagtgc ttatccgctg gcgttaaccc gcgccgccag agctattacc catcgtaccg
    28321 tgctggtggg caatgcggcg caaactctgc acccgattgc cgggcaaggg tttaacctcg
    28381 gtatgcgaga tgtcatgagc cttgcggaaa ccctgactca ggcgcatgag cgcggagaag
    28441 acatcggtga ttacggcata ttgtgccgct atcagcagcg tcgacagagt gatcgcgaag
    28501 caaccatcgg cgtcacggac agcctggtac atctttttgc caaccgttgg gcaccgctgg
    28561 ttgtcgggcg caacatcgga ctgatgacga tggaattatt caccccggca cgcgatgtgc
    28621 tggcgcagcg caccctcggt tgggtggcgc gttgaaggcg gttttaagga ataaacaatg
    28681 caaagtgttg atgtagccat tgttggtggc ggcatggtgg ggctggcggt tgcctgcggc
    28741 ttacagggga gcggcttacg cgttgccgta ctggagcagc gcgtaccgga acctctggcg
    28801 gcggatgcac caccacaact gcgcgtttcg gctatcaatg ccgccagcga aaaattactc
    28861 acccgtcttg gcgtctggca ggacattctc tctcgtaggg ccagttgtta tcacggtatg
    28921 gaagtgtggg acaaagacag ttttggtcac atttcgtttg acgatcaaag catgggctat
    28981 agccatcttg ggcatatcgt tgaaaactca gtgattcact acgcgctgtg gaacaaagcg
    29041 cagcagtcgt cagatatcac tctgttggcc cccgcagaat tacagcaggt cgcctgggga
    29101 gaaaatgaaa ctttcctgac gctgaaagat ggcagcatgt taacggcgcg tctggtgatt
    29161 ggcgcggacg gtgctaattc ctggttgtgc aacaaagcag atattccgct gactttctgg
    29221 gattatcagc atcacgcgct ggtagcgacc attcgcacgg aagaaccgca tgatgcggtg
    29281 gcgcggcagg ttttccatgg cgaaggcatt ctggcctttt taccgcttag cgatccgcat
    29341 ctttgctcaa ttgtctggtc actgtcgcca gaggaagcgc agcggatgca gcaggcaagt
    29401 gaagatgaat ttaatcgcgc gttaaatatc gcttttgata atcgcctggg cttatgcaag
    29461 gttgagagcg cgcgtcaggt gttcccactg acggggcgtt atgcgcgcca gtttgccgcg
    29521 caccgtctgg tgctggtggg cgacgccgcg cataccattc acccgctggc ggggcaggga
    29581 gtaaatctcg gctttatgga tgctgcagag ctgattgctg aactgaaacg gttgcatcgt
    29641 caggggaaag acatcgggca gtacatttac ctgcgccggt atgagcgtag ccgcaagcac
    29701 agtgcggcgc tgatgctggc tggtatgcag ggattccgcg atctgttttc cggtaccaat
    29761 ccggcgaaaa aactgctgcg tgatattggt ttgaaactgg ccgacacgct tcctggcgtt
    29821 aaaccacaac ttatccgcca ggcaatggga ttaaacgatt tgcctgaatg gctgcgttaa
    29881 aaatttctct tctgctgttt atttgatacc catcacactt tcatctcccg gttttttcgc
    29941 cgggagattt tcctcatttg aaataaacta atttcacctc cgttttcgca ttatattttc
    30001 taatgtcacg aatttttgat ttagtgtttt ttgacatttt ttcagctctt a
//
LOCUS       DAZKHF020000029        29753 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2201S31241
            SAMN43267800-rid25106413.denovo.029, whole genome shotgun sequence.
ACCESSION   DAZKHF020000029 DAZKHF020000000
VERSION     DAZKHF020000029.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267800
            Sequence Read Archive: SRR30307263
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 29753)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 29753)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 29753)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350581.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350581.2
            Genome Coverage       :: 151x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:21:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,652
            CDSs (total)                      :: 5,557
            Genes (coding)                    :: 5,320
            CDSs (with protein)               :: 5,320
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 237
            CDSs (without protein)            :: 237
            Pseudo Genes (ambiguous residues) :: 0 of 237
            Pseudo Genes (frameshifted)       :: 102 of 237
            Pseudo Genes (incomplete)         :: 130 of 237
            Pseudo Genes (internal stop)      :: 52 of 237
            Pseudo Genes (multiple problems)  :: 41 of 237
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..29753
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267800-rid25106413.denovo.029"
                     /serotype="O 26:H11"
                     /isolate="2201S31241"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-01"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            32..1321
                     /gene="uraA"
                     /locus_tag="ACHOSC_RS05320"
                     /old_locus_tag="pgaptmp_001064"
     CDS             32..1321
                     /gene="uraA"
                     /locus_tag="ACHOSC_RS05320"
                     /old_locus_tag="pgaptmp_001064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uracil permease"
                     /protein_id="HGU4547044.1"
                     /translation="MTRRAIGVSERPPLLQTIPLSLQHLFAMFGATVLVPVLFHINPA
                     TVLLFNGIGTLLYLFICKGKIPAYLGSSFAFISPVLLLLPLGYEVALGGFIMCGVLFC
                     LVSFIVKKAGTGWLDVLFPPAAMGAIVAVIGLELAGVAAGMAGLLPAEGQTPDSKTII
                     ISITTLAVTVLGSVLFRGFLAIIPILIGVLVGYALSFAMGIVDTTPIINAHWFALPTL
                     YTPRFEWFAILTILPAALVVIAEHVGHLVVTANIVKKDLLRDPGLHRSMFANGLSTVI
                     SGFFGSTPNTTYGENIGVMAITRVYSTWVIGGAAIFAILLSCVGKLAAAIQMIPLPVM
                     GGVSLLLYGVIGASGIRVLIESKVDYNKAQNLILTSVILIIGVSGAKVNIGAAELKGM
                     ALATIVGIGLSLIFKLISVLRPEEVVLDAEDADITDK"
     gene            1416..2117
                     /gene="hda"
                     /locus_tag="ACHOSC_RS05325"
                     /old_locus_tag="pgaptmp_001065"
     CDS             1416..2117
                     /gene="hda"
                     /locus_tag="ACHOSC_RS05325"
                     /old_locus_tag="pgaptmp_001065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416991.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaA inactivator Hda"
                     /protein_id="HGU4547045.1"
                     /translation="MNTPAQLSLPLYLPDDETFASFWPGDNSSLLAALQNVLRQEHSG
                     YIYLWAREGAGRSHLLHAACAELSQRGDAVGYVPLDKRTWFVPEVLDGMEHLSLVCID
                     NIECIAGDELWEMAIFDLYNRILESGKTRLLITGDRPPRQLNLGLPDLASRLDWGQIY
                     KLQPLSDEDKLQALQLRARLRGFELPEDVGRFLLKRLDREMRTLFMTLDQLDRASITA
                     QRKLTIPFVKEILKL"
     gene            complement(2255..2614)
                     /gene="arsC"
                     /locus_tag="ACHOSC_RS05330"
                     /old_locus_tag="pgaptmp_001066"
     CDS             complement(2255..2614)
                     /gene="arsC"
                     /locus_tag="ACHOSC_RS05330"
                     /old_locus_tag="pgaptmp_001066"
                     /EC_number="1.20.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311384.1"
                     /note="This arsenate reductase requires both glutathione
                     and glutaredoxin to convert arsenate to arsenite, after
                     which the efflux transporter formed by ArsA and ArsB can
                     extrude the arsenite from the cell, providing resistance;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0008794 - arsenate reductase
                     (glutaredoxin) activity [Evidence IEA];
                     GO_process: GO:0046685 - response to arsenic-containing
                     substance [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arsenate reductase (glutaredoxin)"
                     /protein_id="HGU4547046.1"
                     /translation="MTKQVKIYHNPRCSKSRETLNLLKENGVEPEVVLYLETPADAAT
                     LRDLLKMLGMNSARELMRQKEDLYKELNLADSSLSEEALIQAMVDNPKLMERPIVVAN
                     GKARIGRPPEQVLEIVG"
     gene            complement(2635..4098)
                     /gene="bepA"
                     /locus_tag="ACHOSC_RS05335"
                     /old_locus_tag="pgaptmp_001067"
     CDS             complement(2635..4098)
                     /gene="bepA"
                     /locus_tag="ACHOSC_RS05335"
                     /old_locus_tag="pgaptmp_001067"
                     /EC_number="3.4.21.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-barrel assembly-enhancing protease"
                     /protein_id="HGU4547047.1"
                     /translation="MFRQLKKNLVATLIAAMTIGQVAPAFADSADTLPDMGTSAGSTL
                     SIGQEMQMGDYYVRQLRGSAPLINDPLLTQYINSLGMRLVSHANSVKTPFHFFLINND
                     EINAFAFFGGNVVLHSALFRYSDNESQLASVMAHEISHVTQRHLARAMEDQQRSAPLT
                     WVGALGSILLAMASPQAGMAALTGTLAGTRQGMISFTQQNEQEADRIGIQVLQRSGFD
                     PQAMPTFLEKLLDQARYSSRPPEILLTHPLPESRLADARNRANQMRPMVVQSSEDFYL
                     AKARTLGMYNSGRNQLTSDLLDEWAKGNVRQQRAAQYGRALQAMEANKYDEARKTLQP
                     LLAAEPGNAWYLDLATDIDLGQNKANEAINRLKNARDLRTNPVLQLNLANAYLQGGQP
                     QEAANILNRYTFNNKDDSNGWDLLAQAEAALNNRDQELAARAEGYALAGRLDQAISLL
                     SSASSQVKLGSLQQARYDARIDQLRQLQERFKPYTKM"
     gene            4311..5372
                     /gene="yfgO"
                     /locus_tag="ACHOSC_RS05340"
                     /old_locus_tag="pgaptmp_001068"
     CDS             4311..5372
                     /gene="yfgO"
                     /locus_tag="ACHOSC_RS05340"
                     /old_locus_tag="pgaptmp_001068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HGU4547048.1"
                     /translation="MLEMLMQWYRRRFSDPEAIALLVILVAGFGIIFFFSGLLAPLLV
                     AIVLAYLLEWPTVRLQSIGCSRRWATSIVLVVFVGILLLMAFVVLPIAWQQGIYLIRD
                     MPGMLNKLSDFAATLPRRYPALMDAGIIDAMAENMRSRMLTMGDSVVKISLASLVGLL
                     TIAVYLVLVPLMVFFLLKDKEQMLNAVRRVLPRNRGLAGQVWKEMNQQITNYIRGKVL
                     EMIVVGIATWLGFLLFGLNYSLLLAVLVGFSVLIPYIGAFVVTIPVVGVALFQFGAGT
                     EFWSCFAVYLIIQALDGNLLVPVLFSEAVNLHPLVIILSVVIFGGLWGFWGVFFAIPL
                     ATLIKAVIHAWPDGQIAQE"
     gene            complement(5410..6258)
                     /gene="focB"
                     /locus_tag="ACHOSC_RS05345"
                     /old_locus_tag="pgaptmp_001069"
     CDS             complement(5410..6258)
                     /gene="focB"
                     /locus_tag="ACHOSC_RS05345"
                     /old_locus_tag="pgaptmp_001069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate transporter FocB"
                     /protein_id="HGU4547049.1"
                     /translation="MRNKLSFDLQLSARKAAIAERIAAHKIARSKVSVFLMAMSAGVF
                     MAIGFTFYLSVIADAPSSQALTHLVGGLCFTLGFILLAVCGTNLFTSSVMTVMAKSRG
                     VISWRTWLINALLVACGNLAGIACFSLLIWFSGLVMSENAMWGVAVLHCAEGKMHHTF
                     TESVSLGIMCNLMVCLALWMSYCGRSLCDKIVAMILPITLFVASGFEHCIANLFVIPF
                     AIAIRHFAPTSFWQLAHSSADNFPALTVSHFITANLLPVMLGNIIGGAVLVSMCYRAI
                     YLRQES"
     gene            complement(6280..8292)
                     /gene="hyfR"
                     /locus_tag="ACHOSC_RS05350"
                     /old_locus_tag="pgaptmp_001070"
     CDS             complement(6280..8292)
                     /gene="hyfR"
                     /locus_tag="ACHOSC_RS05350"
                     /old_locus_tag="pgaptmp_001070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416986.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator HyfR"
                     /protein_id="HGU4547050.1"
                     /translation="MAMSDEAMFAPPQGITIEAVNGMLAERLAQKHGKASLLRAFIPM
                     PPPFSPVQLIELHVLKSNFYYRYHDDGSDVTATTEYQGEMVDYSRHAVLLGSSGMAEL
                     RFIRTHGSRFTPQDCTLFNWLARIITPVLQSWLNDEEQQVALRLLEKDRDHHRVLVDI
                     TNAVLSHLDLDDLIADVAREIHHFFGLASVSMVLGDHRKNEKFSLWCSDLSASHCACL
                     PRNMPGDSVLLTQTLQTRQPTLTHRADDLFLWQRDPLLLLLASNGCESALLIPLTFGN
                     HTPGALLLAHTSSTLFSEENCQLLQHIADRIAIAVGNADAWRSMTDLQESLQQENHQL
                     SEQLLSNLGIGDIIYQSQAMEDLLQQVDIVAKSDSTVLICGETGTGKEVIARAIHQLS
                     PRRDKPLVKINCAAIPASLLESELFGHDKGAFTGAINTHRGRFEIADGGTLFLDEIGD
                     LPLELQPKLLRVLQEREIERLGGSRTIPVNVRVIAATNRDLWQMVEDRQFRSDLFYRL
                     NVFPLELPPLRDRPEDIPLLAKHFTQKMARHMNRSIDAIPTEALRQLMSWDWPGNVRE
                     LENVIERAVLLTRGNSLNLHLNVRQSRLLPTLNEDSALRSSMAQLLHPTTPENDEEER
                     QRIVQVLRETNGIVAGPRGAATRLGMKRTTLLSRMQRLGISVREVL"
     gene            complement(8322..8735)
                     /gene="hyfJ"
                     /locus_tag="ACHOSC_RS05355"
                     /old_locus_tag="pgaptmp_001071"
     CDS             complement(8322..8735)
                     /gene="hyfJ"
                     /locus_tag="ACHOSC_RS05355"
                     /old_locus_tag="pgaptmp_001071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416985.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 assembly chaperone HyfJ"
                     /protein_id="HGU4547051.1"
                     /translation="MTEECGEIVFWTLRKKFVASSDEMPEHSSQVMYYSLAIGHHVGV
                     IDCLNVAFPCPLTEYEDWLAQVEEEQARRKMLGVMTFGEIVIDASHTALLTRAFAPLA
                     DDATSVWQARSIQFIHLLDEIVQEPAIYLMARKIA"
     gene            complement(8728..9485)
                     /gene="hyfI"
                     /locus_tag="ACHOSC_RS05360"
                     /old_locus_tag="pgaptmp_001072"
                     /pseudo
     CDS             complement(8728..9485)
                     /gene="hyfI"
                     /locus_tag="ACHOSC_RS05360"
                     /old_locus_tag="pgaptmp_001072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416984.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 catalytic subunit HyfI"
     gene            complement(9482..10027)
                     /gene="hyfH"
                     /locus_tag="ACHOSC_RS05365"
                     /old_locus_tag="pgaptmp_001073"
     CDS             complement(9482..10027)
                     /gene="hyfH"
                     /locus_tag="ACHOSC_RS05365"
                     /old_locus_tag="pgaptmp_001073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 subunit H"
                     /protein_id="HGU4547052.1"
                     /translation="MLKLLKTIMRAGTATVKYPFAPLEVSPGFRGKPDLMPSQCIACG
                     ACACACPANALTIQTDDQQNSRTWQLYLRRCIYCGRCEEVCPTRAIQLTNNFELTVTN
                     KADLYTRATFHLQRCSRCERPFAPQKTVALAAELLAQQQNAPQNREMLWAQASVCPEC
                     KQRATLLNDDTDVPLVAKEQL"
     gene            complement(10037..11704)
                     /gene="hyfG"
                     /locus_tag="ACHOSC_RS05370"
                     /old_locus_tag="pgaptmp_001074"
     CDS             complement(10037..11704)
                     /gene="hyfG"
                     /locus_tag="ACHOSC_RS05370"
                     /old_locus_tag="pgaptmp_001074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 catalytic subunit HyfG"
                     /protein_id="HGU4547053.1"
                     /translation="MNVNSSSNRGEAILAALKTQFPGAVLDEERQTPEQVTITVKINL
                     LPDVVHYLYYQHDGWLPVLFGNDERTLNGHYAVYYALSMEGAEKCWIVVKALVDADSR
                     EFPSVTPRVPAAVWGEREIRDMYGLIPVGLPDHRRLVLPDDWPEDMHPLRKDAMDYRL
                     RPEPTTDSETYPFINEGNSDAQVIPVGPLHITSDELGHFRLFVDGEQIVDADYRLFYV
                     HRGMEKLAETRMGYNEVTFLSDRVCGICGFAHSVAYTNSVENALGIEVPQRAHTIRSI
                     LLEVERLHSHLLNLGLSCHFVGFDTGFMQFFRVREKSMTMAELLIGSRKTYGLNLIGG
                     VRRDILKEQRLQTLKLVREMRADVSELVEMLLATPNMEQRTQGIGILDRQIARDLRFD
                     HPYADYGNIPKTLFTFTGGDVFSRVMVRVKETFDSLAMLEFALDNMPDTPLLTEGFSY
                     KPHAFALGFVEAPRGEDVHWSMLGDNQKLFRWRCRAATYANWPVLRYMLRGNTVSDAP
                     LIIGSLDPCYSCTDRVTLVDVRKRQSKTVPYKEIERYGIDRNRSPLK"
     gene            complement(11694..13274)
                     /gene="hyfF"
                     /locus_tag="ACHOSC_RS05375"
                     /old_locus_tag="pgaptmp_001075"
     CDS             complement(11694..13274)
                     /gene="hyfF"
                     /locus_tag="ACHOSC_RS05375"
                     /old_locus_tag="pgaptmp_001075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 subunit F"
                     /protein_id="HGU4547054.1"
                     /translation="MSYSVMFALLLLTPLLFSLLCFACRKRGLSATCTVTVLHSLGIT
                     LLLILALWVVQTAADAGEIFAAGLWLHIDGLGGLFLAILGVIGFLTGVYSIGYMRHEV
                     AHGELSPVTLCDYYGFFHLFLFTMLLVVTSNNLIVMWAAIEATTLSSAFLVGIYGQRS
                     SLEAAWKYIIICTVGVAFGLFGTVLVYANVASVMPQAEMAIFWSEVLKQSSLLDPTLM
                     LLAFVFVLIGFGTKTGLFPMHAWLPDAHSEAPSPVSALLSAVLLNCALLVLIRYYIII
                     CQAIGSDFPNRLLLIFGMLSVAVAAFFILVQRDIKRLLAYSSVENMGLVAVALGIGGP
                     LGIFAALLHTLNHSLAKTLLFCGSGNVLLKYGTRDLNVVCGMLKIMPFTAVLFGGGAL
                     ALAGMPPFNIFLSEFMTITAGLARNHLLIIVLLLLLLTLVLAGLVRMAARVLMAKPPQ
                     AVNRGDLGWLTTSPMVILLVMMLAMGTHIPQPVIRILAGASTIVLSGTHDLPAQRSTW
                     HDFLPSGTASVSEKHSER"
     gene            complement(13279..13929)
                     /gene="hyfE"
                     /locus_tag="ACHOSC_RS05380"
                     /old_locus_tag="pgaptmp_001076"
     CDS             complement(13279..13929)
                     /gene="hyfE"
                     /locus_tag="ACHOSC_RS05380"
                     /old_locus_tag="pgaptmp_001076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 membrane subunit"
                     /protein_id="HGU4547055.1"
                     /translation="MTGSMIVNNLAGLMMLTSLFVISVKSYRLSCGFYACQSLVLVSI
                     FATLSCLFAAEQLLIWSASAFITKVLLVPLIMTYAARNIPQNIPEKALFGPAMMALLA
                     ALIVLLCAFVVQPVKLPMATGLKPALAVALGHFLLGLLCIVSQRNILRQIFGYCLMEN
                     GSHLVLALLAWRAPELVEIGIATDAIFAVIVMVLLARKIWRTHGTLDVNNLTALKG"
     gene            complement(13941..15380)
                     /gene="hyfD"
                     /locus_tag="ACHOSC_RS05385"
                     /old_locus_tag="pgaptmp_001077"
     CDS             complement(13941..15380)
                     /gene="hyfD"
                     /locus_tag="ACHOSC_RS05385"
                     /old_locus_tag="pgaptmp_001077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 subunit HyfD"
                     /protein_id="HGU4547056.1"
                     /translation="MENLALTTLLLPFIGALVVSFSPQRRAAEWGVLFAALTTLCMLS
                     LISAFYQADKVAVTLTLVNVGDVALFGLVIDRVSTLILFVVVFLGLLVTIYSTGYLTD
                     KNREHPHNGTNRYYAFLLLFIGAMAGLVLSSTLLGQLLFFEITGGCSWALISYYQSDK
                     AQRSALKALLITHIGSLGLYLAAATLFLQTGTFALSAMSELHGDARYLVYGGILFAAW
                     GKSAQLPMQAWLPDAMEAPTPISAYLHAASKVKVGVYIFARAIIDGGNIPHVIGGVGM
                     VMALVTILYGFLMYLPQQDMKRLLAWSTITQLGWMFFGLSLSIFGSRLALEGSIAYIV
                     NHAFAKSLFFLVAGALSYSCGTRLLPRLRGVLHTLPLPGVGFCVAALAITGVPPFNGF
                     FSKFPLFAAGFALSVEYWILLPAMILLMIESVASFAWFIRWFGRVVPGKPSEAVADAA
                     PLPGSMRLVLIVLIVMSLISSVIAATWLQ"
     gene            complement(15397..16344)
                     /gene="hyfC"
                     /locus_tag="ACHOSC_RS05390"
                     /old_locus_tag="pgaptmp_001078"
     CDS             complement(15397..16344)
                     /gene="hyfC"
                     /locus_tag="ACHOSC_RS05390"
                     /old_locus_tag="pgaptmp_001078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416978.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 subunit HyfC"
                     /protein_id="HGU4547057.1"
                     /translation="MRQTLCDGYLVIFALAQAVILLMLTPLFTGISRQIRARMHSRRG
                     PGIWQDYRDIHKLFKRQEVAPTSSGLMFRLMPWVLISSMLVLAMALPLFITVSPFAGG
                     GDLITLIYLLALFRFFFALSGLDTGSPFAGVGASRELTLGILVEPMLILSLLVLALIA
                     GSTHIEMISNTLAMGWNSPLTTVLALLACGFACFIEMGKIPFDVAEAEQELQEGPLTE
                     YSGAGLALAKWGLGLKQVVMASLFVALFLSFGRAQELSLACLLTSLVVTLLKVLLIFV
                     LASIAENTLARGRFLLIHHVTWLGFSLAALAWVFWLTGL"
     gene            complement(16355..18373)
                     /gene="hyfB"
                     /locus_tag="ACHOSC_RS05395"
                     /old_locus_tag="pgaptmp_001079"
     CDS             complement(16355..18373)
                     /gene="hyfB"
                     /locus_tag="ACHOSC_RS05395"
                     /old_locus_tag="pgaptmp_001079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_process: GO:0042773 - ATP synthesis coupled electron
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 subunit B"
                     /protein_id="HGU4547058.1"
                     /translation="MDALQLLTWSLILYLFASLASLFLLGLDRLAIKLSGITSLVGGV
                     IGIISGITQLHAGVTLVARFATPFDFADFTLRMDSLSAFMVLVISLLVVVCSLYSLTY
                     MREYEGKGAAAMGFFMNLFIASMVALLVMDNAFWFIVLFEMMSLSSWFLVIARQDKTS
                     INAGMLYFFIAHAGSVLIMIAFLLMGRESGSLDFASFRTLSLSPGLASAVFLLAFFGF
                     GAKAGMMPLHSWLPRAHPAAPSHASALMSGVMVKIGIFGILKVAMDLLAQTGLPLWWG
                     ILVMAIGAISALLGVLYALAEQDIKRLLAWSTVENVGIILLAVGVAMVGLSLHDPLLT
                     VVGLLGALFHLLNHALFKGLLFLGAGAIISRLHTHDMEKMGALAKRMPWTAAACLIGC
                     LAISAIPPLNGFISEWYTWQSLFSLSRVEAVALQLAGPIAMVMLAVTGGLAVMCFVKM
                     YGITFCGAPRSTHAEEAQEVPNTMIVAMLLLAALCVLIALSASWLAPKIMHIAHAFTN
                     TPPVTVASGIALVPGTFHTQVTPSLLLLLLLAMPLLPGLYWLWCRSRRAAFRRTGDAW
                     ACGYGWENAMAPSGNGVMQPLRVVFSALFRLRQQLDPTLRLNKGFAHVTARAQSTEPF
                     WDERVIRPIVSATQRLAKEIQHLQSGDFRLYCLYVVAALVVLLIAIAV"
     gene            complement(18373..18990)
                     /gene="hyfA"
                     /locus_tag="ACHOSC_RS05400"
                     /old_locus_tag="pgaptmp_001080"
     CDS             complement(18373..18990)
                     /gene="hyfA"
                     /locus_tag="ACHOSC_RS05400"
                     /old_locus_tag="pgaptmp_001080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311370.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 subunit HyfA"
                     /protein_id="HGU4547059.1"
                     /translation="MNRFVVAEPLWCTGCNTCLAACSDVHKTQGLQQHPRLALAKTST
                     LTAPVVCHHCEEAPCLQVCPVNAISQRDDAIQLNESLCIGCKLCAVVCPFGAISASGS
                     RPVNAPAQYVFQAEGSLKDGEENVPPQHALLRWEPGVQTVAVKCDLCDFLPEGPACVR
                     ACPNQALRLITDDSLQRQMKEKQRLAASWFANGGEDPLSLTQEQR"
     gene            complement(19243..19713)
                     /gene="bcp"
                     /locus_tag="ACHOSC_RS05405"
                     /old_locus_tag="pgaptmp_001081"
     CDS             complement(19243..19713)
                     /gene="bcp"
                     /locus_tag="ACHOSC_RS05405"
                     /old_locus_tag="pgaptmp_001081"
                     /EC_number="1.11.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016209 - antioxidant activity [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-dependent thiol peroxidase"
                     /protein_id="HGU4547060.1"
                     /translation="MNPLKAGDIAPKFSLPDQDGEQVNLTDFQGQRVLVYFYPKAMTP
                     GCTVQACGLRDNMDELKKAGVDVLGISTDKPEKLSRFAEKELLNFTLLSDEDHQVCEQ
                     FGVWGEKSFMGKTYDGIHRISFLIDADGKIEHVFDDFKTSNHHDVVLNWLKEHA"
     gene            complement(19713..20285)
                     /gene="gcvR"
                     /locus_tag="ACHOSC_RS05410"
                     /old_locus_tag="pgaptmp_001082"
     CDS             complement(19713..20285)
                     /gene="gcvR"
                     /locus_tag="ACHOSC_RS05410"
                     /old_locus_tag="pgaptmp_001082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system transcriptional
                     repressor"
                     /protein_id="HGU4547061.1"
                     /translation="MTLSSQHYLVITALGADRPGIVNTITRHVSSCGCNIEDSRLAML
                     GEEFTFIMLLSGSWNAITLIESTLPLKGAELDLLIVMKRTTARPRPPMPASVWVQVDV
                     ADSPHLIERFTALFDAHHMNIAELVSRTQPAENERAAQLHIQITAHSPASADAANIEQ
                     AFKALCTELNAQGSINVVNYSQHDEQDGVK"
     gene            20431..21309
                     /gene="dapA"
                     /locus_tag="ACHOSC_RS05415"
                     /old_locus_tag="pgaptmp_001083"
     CDS             20431..21309
                     /gene="dapA"
                     /locus_tag="ACHOSC_RS05415"
                     /old_locus_tag="pgaptmp_001083"
                     /EC_number="4.3.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001311023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008840 - 4-hydroxy-tetrahydrodipicolinate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate synthase"
                     /protein_id="HGU4547062.1"
                     /translation="MFTGSIVAIVTPMDEKGNVCRASLKKLIDYHVASGTSAIVSVGT
                     TGESATLNHDEHADVVMMTLDLADGRIPVIAGTGANATAEAISLTQRFNDSGIVGCLT
                     VTPYYNRPSQEGLYQHFKAIAEHTDLPQILYNVPSRTGCDLLPETVGRLAKVKNIIGI
                     KEATGNLTRVNQIKELVSDDFVLLSGDDASALDFMQLGGHGVISVTANVAARDMAQMC
                     KLAAEGHFAEARVINQRLMPLHNKLFVEPNPIPVKWACKELGLVATDTLRLPMTPITD
                     SGRETVRAALKHAGLL"
     gene            21326..22360
                     /gene="bamC"
                     /locus_tag="ACHOSC_RS05420"
                     /old_locus_tag="pgaptmp_001084"
     CDS             21326..22360
                     /gene="bamC"
                     /locus_tag="ACHOSC_RS05420"
                     /old_locus_tag="pgaptmp_001084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708316.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamC"
                     /protein_id="HGU4547063.1"
                     /translation="MAYSVQKSRLAKVAGVSLVLLLAACSSDSRYKRQVSGDEAYLEA
                     APLAELHAPAGMILPVTSGDYAIPVTNGSGAVGKALDIRPPAQPLALVSGARTQFTGD
                     TASLLVENGRGNTLWPQVVSVLQAKNYTITQRDDAGQTLTTDWVQWNRLDEDEQYRGR
                     YQISVKPQGYQQAVTVKLLNLEQAGKPVADAASMQRYSTEMMNVISAGLDKSATDAAN
                     AAQNRASTTMDVQSAADDTGLPMLVVRGPFNVVWQRLPAALEKVGMKVTDSTRSQGNM
                     AVTYKPLSDSDWQELGASDPGLASGDYKLQVGDLDNRSSLQFIDPKGHTLTQSQNDAL
                     VAVFQAAFSK"
     gene            22573..23286
                     /gene="purC"
                     /locus_tag="ACHOSC_RS05425"
                     /old_locus_tag="pgaptmp_001085"
     CDS             22573..23286
                     /gene="purC"
                     /locus_tag="ACHOSC_RS05425"
                     /old_locus_tag="pgaptmp_001085"
                     /EC_number="6.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017458944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004639 -
                     phosphoribosylaminoimidazolesuccinocarboxamide synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylaminoimidazolesuccinocarboxamide
                     synthase"
                     /protein_id="HGU4547064.1"
                     /translation="MQKQAELYRGKAKTVYSTENPDLLVLEFRNDTSAGDGARIEQFD
                     RKGMVNNKFNYFIMSKLAEAGIPTQMERLLSDTECLVKKLDMVPVECVVRNRAAGSLV
                     KRLGIEEGIELNPPLFDLFLKNDAMHDPMVNESYCETFGWVSKENLARMKELTYKAND
                     VLKKLFDDAGLILVDFKLEFGLYKGEVVLGDEFSPDGSRLWDKETLEKMDKDRFRQSL
                     GGLIEAYEAVARRLGVQLD"
     gene            23440..23667
                     /gene="vapB"
                     /locus_tag="ACHOSC_RS05430"
                     /old_locus_tag="pgaptmp_001086"
     CDS             23440..23667
                     /gene="vapB"
                     /locus_tag="ACHOSC_RS05430"
                     /old_locus_tag="pgaptmp_001086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxin-antitoxin system antitoxin VapB"
                     /protein_id="HGU4547065.1"
                     /translation="METTVFLSNRSQAVRLPKAVALPEDVKKVEIIAIGRTRIITPAG
                     ESWDSWFDGENVSADFMDIRDQPAMQERESF"
     gene            23667..24065
                     /locus_tag="ACHOSC_RS05435"
                     /old_locus_tag="pgaptmp_001087"
     CDS             23667..24065
                     /locus_tag="ACHOSC_RS05435"
                     /old_locus_tag="pgaptmp_001087"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_490590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004540 - RNA nuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system VapC family
                     toxin"
                     /protein_id="HGU4547066.1"
                     /translation="MLKFMLDTNICIFTIKNKPVHVRERFNLNSGRMCISSVTLMELI
                     YGAEKSQMPERNLAVIEGFVSRLVVLDYDSAAATHTGQIRAELARQGRPVGPFDQMIA
                     GHARSRGLIVVTNNTREFERVAGIRLEDWS"
     gene            24212..25075
                     /gene="ypfJ"
                     /locus_tag="ACHOSC_RS05440"
                     /old_locus_tag="pgaptmp_001088"
     CDS             24212..25075
                     /gene="ypfJ"
                     /locus_tag="ACHOSC_RS05440"
                     /old_locus_tag="pgaptmp_001088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="neutral zinc metallopeptidase"
                     /protein_id="HGU4547067.1"
                     /translation="MRWQGRRESDNVEDRRNSSGGPSMGGPGFRLPSGKGGLILLLVV
                     LVAGYYGVDLTGLMTGQPVSQQQSTRSISPNEDEAAKFTSVILATTEDTWGQQFEKMG
                     KTYQQPKLVMYRGMTRTGCGAGQSIMGPFYCPADGTVYIDLSFYDDMKDKLGADGDFA
                     QGYVIAHEVGHHVQKLLGIEPKVRQLQQNATQAEVNRLSVRMELQADCFAGVWGHSMQ
                     QQGVLETGDLEEALNAAQAIGDDRLQQQSQGRVVPDSFTHGTSQQRYSWFKRGFDSGD
                     PAQCNTFGKSI"
     gene            25090..27105
                     /gene="tmcA"
                     /locus_tag="ACHOSC_RS05445"
                     /old_locus_tag="pgaptmp_001089"
     CDS             25090..27105
                     /gene="tmcA"
                     /locus_tag="ACHOSC_RS05445"
                     /old_locus_tag="pgaptmp_001089"
                     /EC_number="2.3.1.193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA cytosine(34) acetyltransferase TmcA"
                     /protein_id="HGU4547068.1"
                     /translation="MAELTALHTLTAQMKREGIRRLLVLSGEEGWCFDHALKLRDALP
                     GDWLWISPQPDAENHCSPSALQTLLGREFRHAVFDARHGFDAAAFAALSGTLKAGSWL
                     VLLLPVWEEWENQPDADSLRWSDCPDPIATPHFVQHLKRVLTADNDAILWRQNQPFSL
                     AHFTPRTDWHPATGAPQPEQQQLLQQLLTMPPGVAAVTAARGRGKSALAGQLISRIAG
                     SAIVTAPAKAATDVLAQFAGEKFRFIAPDALLASDEQADWLVVDEAAAIPAPLLHQLV
                     SRFPRTLLTTTVQGYEGTGRGFLLKFCARFPHLHRFELQQPIRWAQGCPLEKMVSEAL
                     VFDDENFTHTPQGNIVISAFEQTLWRSEPETPLKVYQLLSGAHYRTSPLDLRRMMDAP
                     GQHFLQAAGENEIAGALWLVDEGGLSQQLSQAVWAGFRRPRGNLVAQSLAAHGSNPLA
                     ATLRGRRVSRIAVHPARQREGTGQQLIAGALQYTRDLDYLSVSFGYTGELWRFWHRCG
                     FVLVRMGNHREASSGCYTAMALLPMSNAGKQLAEREHYRLRRDAQALAKWNGETLPVD
                     PLNDAVLSDDDWLELAGFAFAHRPLLTSLGCLLRLLQTSELALPALRGRLQKNVSDAQ
                     LCTTLKLSGRKMLLVRQREEAAQALFALNDVRTERLRDRITQWQFFH"
     gene            27179..27877
                     /gene="ypfH"
                     /locus_tag="ACHOSC_RS05450"
                     /old_locus_tag="pgaptmp_001090"
     CDS             27179..27877
                     /gene="ypfH"
                     /locus_tag="ACHOSC_RS05450"
                     /old_locus_tag="pgaptmp_001090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311362.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="esterase"
                     /protein_id="HGU4547069.1"
                     /translation="MKHDHFVVQSPDKPAQQLLLLFHGVGDNPVAMGEIGSWFAPLFP
                     DALVVSVGGAEPSGNPAGRQWFSVQGITEDNRQARVDAIMPTFIETVRYWQKQSGVGA
                     NATALIGFSQGAIMALESIKAEPGLASRVIAFNGRYASLPETASTATTIHLIHGGEDP
                     VIDLAHAVAAQEALISAGGDVTLDIVEDLGHAIDNRSMQFALDHLRYTIPKHYFDEAL
                     SGGKPGDDDVIEMM"
     gene            complement(27987..28187)
                     /gene="ypfN"
                     /locus_tag="ACHOSC_RS05455"
                     /old_locus_tag="pgaptmp_001091"
     CDS             complement(27987..28187)
                     /gene="ypfN"
                     /locus_tag="ACHOSC_RS05455"
                     /old_locus_tag="pgaptmp_001091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YpfN family protein"
                     /protein_id="HGU4547070.1"
                     /translation="MDWLAKYWWILVIVFLVGVLLNVIKDLKRVDHKKFLANKPELPP
                     HRDFNDKWDDDDDWPKKDQPKK"
     gene            complement(28215..29342)
                     /gene="dapE"
                     /locus_tag="ACHOSC_RS05460"
                     /old_locus_tag="pgaptmp_001092"
     CDS             complement(28215..29342)
                     /gene="dapE"
                     /locus_tag="ACHOSC_RS05460"
                     /old_locus_tag="pgaptmp_001092"
                     /EC_number="3.5.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020078199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009014 - succinyl-diaminopimelate
                     desuccinylase activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinyl-diaminopimelate desuccinylase"
                     /protein_id="HGU4547071.1"
                     /translation="MSCPVIELTQQLIRRPSLSPDDAGCQALLIERLQAIGFTVERMD
                     FADTQNFWAWRGQGETLAFAGHTDVVPPGDADRWINPPFEPTIRDGMLFGRGAADMKG
                     SLAAMVVAAERFVAQHPNHTGRLAFLITSDEEASAHNGTVKVVEALMARNERLDYCLV
                     GEPSSIEVVGDVVKNGRRGSLTCNLTIHGVQGHVAYPHLADNPVHRAAPFLNELVAIE
                     WDQGNEFFPATSMQIANIQAGTGSNNVIPGELFVQFNFRFSTELTDEMIKAQVLALLE
                     KHQLRYTVDWWLSGQPFLTARGKLVDAVVNAVEHYNEIKPQLLTTGGTSDGRFIARMG
                     AQVVELGPVNATIHKINECVNAANLQLLARMYQRIMEQLVA"
     gene            complement(29346..29702)
                     /gene="yffB"
                     /locus_tag="ACHOSC_RS05465"
                     /old_locus_tag="pgaptmp_001093"
     CDS             complement(29346..29702)
                     /gene="yffB"
                     /locus_tag="ACHOSC_RS05465"
                     /old_locus_tag="pgaptmp_001093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArsC family reductase"
                     /protein_id="HGU4547072.1"
                     /translation="MVTLYGIKNCDTIKKARRWLEANNIDYRFHDYRVDGLDSELLNG
                     FINELGWEALLNTRGTTWRKLDETTRNKITDAASAAALMTEMPAIIKRPLLCAPGKPM
                     LLGFSDSSYQQFFHEV"
BASE COUNT         7202 a         7751 c         7986 g         6814 t
ORIGIN      
        1 gcctataaca cataatacag aggataatac tatgacgcgc cgtgctatcg gggtgagtga
       61 aagaccgcca cttttacaga caatcccgct tagtttgcaa catttgttcg ccatgtttgg
      121 tgcaaccgtc ctggtgcccg tcttatttca tattaacccg gcgactgtac tgttatttaa
      181 cggtattgga acgctgctgt atctcttcat ctgtaaaggg aaaattccgg cttatcttgg
      241 ttccagcttt gcctttattt caccggtatt gttactgttg ccgttagggt atgaagtcgc
      301 gctgggcggc tttattatgt gcggcgtgct gttctgcctg gtttctttta tcgtgaagaa
      361 agcggggacc ggctggctgg acgtgctgtt tccacctgcg gcaatgggcg caatcgttgc
      421 cgtcatcggt ctggagctgg cgggcgtagc tgccggtatg gcgggtttac tcccggctga
      481 agggcaaacg ccagactcca aaaccatcat catctctatt accaccctgg cggtcacggt
      541 tttaggttcc gtgctgtttc gtggtttcct ggcaattatc ccgattttaa ttggcgtgct
      601 ggtggggtac gcgctctctt tcgcaatggg aattgtcgat accacgccga ttattaatgc
      661 tcactggttt gcgctgccaa ccctctatac gccgcgcttc gagtggtttg ccattctgac
      721 tattctgcca gcggcgttag tggttattgc cgaacacgta gggcacctgg tagtaacggc
      781 taatatcgtc aaaaaagatc tgctgcgcga tccaggtctg caccgttcga tgtttgctaa
      841 tggcttgtcg accgtgattt ccggcttctt tggctctacg ccaaatacca cttacggaga
      901 aaacattggc gtgatggcga tcacccgtgt ttacagtacc tgggttatcg gcggggcggc
      961 gattttcgct atcctgcttt cctgcgtcgg taaactggct gccgctatcc agatgatccc
     1021 attgccggtg atgggcggcg tttcgctgct gctttatggt gtcatcggtg cttccggtat
     1081 tcgtgttttg atcgaatcga aagtggatta caacaaagca cagaacctga tcctgacttc
     1141 cgtgattttg atcatcggcg tcagtggcgc gaaggtaaac atcggcgcgg cggagttaaa
     1201 aggtatggcg ctggcgacca tcgtcggtat cggcttaagt ttgatcttca aactgatcag
     1261 tgtgctgcgc cctgaagaag tggtgctgga cgcagaagac gcggatataa cagacaaata
     1321 atgtaccgac cgggcagtgt tgctgcccgg ttcaaacatc atgggattct gtggtaaact
     1381 tctcgcgatt ttgtgaaatc ctggttgagg tatctctgaa cacaccggca cagctctctt
     1441 tgccacttta tcttcctgac gacgaaacct ttgcaagttt ctggccgggg gataactcct
     1501 ctttactggc tgcgctgcaa aacgtgctgc gtcaggaaca tagcggttac atctatctct
     1561 gggcacgcga aggcgcgggg cgcagccatc tgctgcacgc ggcttgcgcg gaattgtcgc
     1621 agcgtggcga tgcggtgggc tatgtcccgc tggataaacg cacctggttt gttccggaag
     1681 tgctcgacgg tatggagcat ttgtcgctgg tctgtatcga caacattgag tgtattgcag
     1741 gcgatgagtt gtgggagatg gcgattttcg atctctacaa tcgaattctg gaatcgggca
     1801 aaacacgatt gttgatcacc ggcgatcgtc caccgcggca gttgaatctg ggattaccgg
     1861 atctcgcgtc gcgactcgac tgggggcaga tctacaaatt gcagccactt tctgatgaag
     1921 ataagttgca ggcgctacag ttacgcgcgc gtttgcgtgg ttttgaactg ccggaagatg
     1981 tggggcgttt cttgctgaag cggctcgaca gagaaatgcg cacgctattt atgacgttgg
     2041 atcagttgga tcgtgcgtcg attaccgcgc aacgtaagct gaccattccg tttgtgaaag
     2101 aaattctgaa gttgtagata aggtgtttat tgtcggatgc ggcgcgaacg ccttatccga
     2161 actacgcgaa gttaatccat agtattcgta ggccggataa gacgtggcaa gcgtcgcatc
     2221 cggcaatgtg cttcaactag cgatggagca catcttaacc cacgatttcc agtacctgct
     2281 ccggtggacg accaatccgc gctttgccat tcgcgaccac aatcgggcgc tccattagct
     2341 tcggattatc caccattgcc tgaatcagcg cctcttcgct aagcgaactg tcagccaggt
     2401 tcagttcttt ataaagatcc tctttctggc gcatcaattc acgggcgcta ttcatcccca
     2461 gcattttcag caaatcgcgc agcgttgccg catcggcggg tgtctcaagg tagagtacca
     2521 cttctggctc cacgccgttt tctttcagca ggttcagcgt ttcccggctc tttgaacagc
     2581 gtgggttatg gtagattttt acctgtttgg tcattgttct tcctttaatg cgaattacat
     2641 cttggtataa ggcttaaagc gttcctgcag ctggcgcaac tggtcgatgc gcgcatcgta
     2701 acgcgcttgt tgcaggctgc ctaatttcac ctgcgaactg gcgctactca acagcgaaat
     2761 ggcctgatcg agtcgtccgg cgagcgcata accttctgct cgcgcagcca gctcctgatc
     2821 gcggttattt agcgcggctt ccgcctgtgc cagtaaatcc cagccgttgc tgtcatcttt
     2881 attattaaag gtgtagcgat tcagaatatt ggccgcttct tgtggttgac cgccttgtag
     2941 ataagcgttc gccaggttga gctgcaacac cggattggtg cgcaaatcac gggcattttt
     3001 caggcgattg attgcctcat tggctttgtt ttgcccaaga tcgatatcgg tagccagatc
     3061 gagataccat gcgttgccag gttctgccgc cagtaacggt tgcagcgttt tacgcgcctc
     3121 gtcgtattta ttggcttcca tcgcctgtaa agcacgacca tattgcgccg ctcgttgctg
     3181 acgaacgttt cctttcgccc attcatccag caaatcactg gtgagctggt tacgtccgga
     3241 attatacatc cccagtgtgc gcgctttcgc cagatagaaa tcttccgacg actgcaccac
     3301 catcggtcgc atctgattag cacggttgcg ggcatctgcc agacgacttt ccggcaacgg
     3361 gtgagtcaat aaaatttccg gcgggcgcga ggagtaacgc gcctgatcga gtaatttttc
     3421 gaggaaggtt ggcatcgcct gcggatcgaa tcccgagcgt tgcagcacct gaataccaat
     3481 gcggtccgct tcctgttcat tttgctgggt gaaactgatc attccctgac gtgttcccgc
     3541 cagtgtaccg gtcagcgccg ccatccccgc ctgcggactg gccatcgcca gtaaaataga
     3601 acctaacgcg ccgacccagg tcagcggcgc gctgcgttgt tgatcttcca tcgctcgcgc
     3661 caggtgacgt tgggtgacgt gggagatttc gtgcgccata actgaagcca gttgactttc
     3721 gttatcggaa taacggaaca gggcagagtg cagcaccacg ttgccgccaa agaaagcaaa
     3781 ggcgttaatt tcgtcgttgt tgatcagaaa aaaatgaaac ggtgtcttaa ccgaattggc
     3841 atgcgaaacc agacgcatcc ccagcgaatt aatatattgc gttaacagcg ggtcattaat
     3901 taacggcgcg ctgccgcgta gctggcggac ataatagtcg cccatctgca tttcctgacc
     3961 aatggaaagc gtgcttcctg cggaggttcc catatccggc aaggtgtctg cgctgtcggc
     4021 aaacgccggg gctacctgac caatggtcat agcagcaatg agggttgcaa ccaggttttt
     4081 tttcaactgc ctgaacataa cctctatcct gtatttctga agatagtcat ttgaccgata
     4141 ctgttaaata tcgttcccac cgcgccgcct ttgagtgtaa cggcgaagat tagcccactc
     4201 aaatgacttt tcagtttcct gaatccgtca taaaaagcga agtctttttt gtgacatttc
     4261 gatacaattg cggatcgcaa tcccgctctt gaggttcagg gaaggttttt atgctcgaaa
     4321 tgttgatgca atggtatcgc cgccgtttta gcgacccgga agcgattgcc ttgctggtta
     4381 ttttagttgc cggatttggc attatctttt tctttagtgg cctgcttgct ccgttgctgg
     4441 tggctattgt gctggcctat ttgctggaat ggccaaccgt gcgcctgcaa tctattggct
     4501 gctcccgccg ctgggcgacg tcgattgtat tggtggtttt tgtcggtata ttgctactga
     4561 tggcgttcgt ggtactgcct atcgcctggc aacagggcat ctacttaatc cgcgatatgc
     4621 cggggatgct caataagctt tctgactttg ccgccacgtt gccgcgccgc tatccggcgt
     4681 taatggacgc gggcattatt gatgcaatgg ccgaaaatat gcgcagtcgg atgctgacca
     4741 tgggcgattc ggtggtgaaa atttccctcg cctcgctggt cggtttgctg accattgccg
     4801 tctatctggt gctggtgcca ttgatggtct tcttcctgct gaaagacaaa gagcagatgc
     4861 tgaacgccgt tcgtcgggtg ctgccgcgta accgtgggct ggcaggacag gtgtggaagg
     4921 agatgaatca acaaatcacc aactatatcc gcggcaaagt gctggagatg atcgtggtag
     4981 ggatcgccac ctggctgggg ttcttgctct tcgggctgaa ctattcgctg ctgctggcgg
     5041 tgctggtcgg cttctcggtt cttattccgt acattggcgc atttgtggtg accattccgg
     5101 tggttggcgt ggcgctattc cagtttggcg caggcacgga attctggagc tgcttcgcgg
     5161 tgtatctgat tattcaggcg ctggacggca acctgctagt accggtgttg ttctccgaag
     5221 cggttaacct gcatccgctg gtgattattt tatcggtggt gatcttcggt ggtttgtggg
     5281 gattctgggg cgtattcttc gccattccat tggcgacgct gatcaaagcc gtgatccacg
     5341 cctggcccga tgggcaaatc gcgcaagaat aatcttcagc ccgacgaact gtttcgccgg
     5401 gctgattttt caggattcct gacgtaaata aatagcccga taacacatac tcaccagcac
     5461 tgcaccgccg ataatattac ccagcatcac cgggagcaga ttggcggtaa taaaatggct
     5521 gaccgtcagt gccggaaaat tgtctgcgct actgtgcgcc agttgccaga aggaggtagg
     5581 ggcgaaatgg cgaatggcaa tggcgaacgg aatcacaaac aaattggcga tacagtgctc
     5641 aaagccactg gcgacaaaca gggtgatggg caaaatcatg gcgacgattt tgtcgcataa
     5701 cgaacgcccg caataactca tccacagcgc caggcaaacc atcagattgc acataatgcc
     5761 gaggctgaca gattcagtaa atgtatgatg cattttgccc tcggcgcagt gtaaaaccgc
     5821 gactccccac atcgcgtttt cactcatcac cagcccggaa aaccagatta acaaactgaa
     5881 acaggcaata cctgccagat taccgcaggc caccagaagt gcgttaatca gccaagttcg
     5941 ccaactaata acgcccctac tttttgccat taccgtcatt accgacgagg tgaacaggtt
     6001 ggtgccgcaa accgccagca aaataaagcc aagtgtaaag caaaggccgc ccaccagatg
     6061 ggttaatgcc tgtgaagacg gggcatcggc gataactgaa aggtaaaaag taaatccgat
     6121 cgccataaac acgccagcgg acatcgccat taaaaagacc gacactttac tgcgggcaat
     6181 tttatgggcg gcaatccgtt cagcgattgc cgcttttctg gcgctcaact gcaagtcgaa
     6241 agagagtttg tttctcatgc atactcctgc aaaagcagat tacaacacct cgcgaaccga
     6301 gattcccaga cgctgcattc gtgacagcag cgtggtgcgc ttcatcccta atcgtgtcgc
     6361 cgcgccacgg ggcccggcaa caatgccatt ggtttctcgc aatacctgaa caatgcgctg
     6421 acgttcttct tcgtcattct ctggcgtcgt cgggtgcagc aactgcgcca ttgaactgcg
     6481 aagcgctgaa tcttcattta gcgtcggcag taaacggctt tgtcggacat taagatgtaa
     6541 attcagactg ttaccgcgag tcagcagtac cgctcgctca atcacgtttt ccagctcgcg
     6601 cacgttgccc ggccagtccc acgacatcaa ctggcgtagc gcctcggtcg ggatggcgtc
     6661 aattgaacga ttcatatggc gcgccatttt ttgcgtgaaa tgctttgcta aaagagggat
     6721 atcttccgga cggtcgcgca gcggcggcaa ttccagtggg aagacattca ggcgataaaa
     6781 gagatcgctg cgaaactggc gatcttcaac catttgccac aaatcacggt tggtggcggc
     6841 aatgactctg acatttaccg ggatcgttct actcccgccg agacgctcaa tctcccgttc
     6901 ctgcaatacg cgcagcagtt taggctgaag ttctaacggc agatcgccaa tttcatcgag
     6961 aaacaacgtg ccgccatcgg caatttcaaa acgaccacga tgggtattaa tcgcaccagt
     7021 aaacgcccct ttgtcatgac cgaataactc actttccaga agactggcgg ggatggcagc
     7081 gcagttgatt ttgaccagcg gcttgtcgcg tcgcgggcta agttgatgga tcgctctggc
     7141 gatcacctct ttgccggttc cggtttcacc gcaaatcaac accgtactgt cgctcttcgc
     7201 cacaatatct acctgctgga gtaggtcttc cattgcctgg ctttgataga taatgtcacc
     7261 gatgcccaga ttcgaaagga gctgctcgct aagctggtgg ttttcttgct gcaaactttc
     7321 ctgcaaatcg gtcatgctac gccaggcatc ggcattgcca acggcaatag cgatgcgatc
     7381 ggctatgtgt tgtagtagct ggcagttttc ctcactaaag agagtggaag aggtatgcgc
     7441 cagcaacaat gcacccggtg tatggttgcc aaaggtaagc ggtataagga gcgcagattc
     7501 gcagccgtta gatgcaagta agagtaataa cgggtcgcgt tgccagagaa acagatcgtc
     7561 tgcacggtgc gtcaaggtcg gttgtcgggt ttgtagcgtt tgtgtcagca atacactgtc
     7621 gccaggcata ttgcgtggca gacacgcaca atgtgaggca gaaagatcgc tgcaccacag
     7681 gctaaacttc tcgttctttc gatgatcgcc cagtaccata ctgactgaag ccagaccgaa
     7741 aaaatgatgg atctcacgag cgacgtcagc gatcagatcg tcgagatcaa gatgtgacag
     7801 cactgcatta gtaatatcaa ccagtacccg atgatgatcg cgatctttct ccagcaaacg
     7861 cagcgccacc tgctgttctt catcattgag ccatgattgc agaaccgggg tgattatccg
     7921 cgccagccag ttaaacagtg tgcaatcctg gggagtaaaa cggctgccgt gggtacgaat
     7981 aaagcgtagc tccgccattc cactactgcc gagaaggacg gcgtgacgcg aataatcgac
     8041 catctcgccc tgatactctg ttgttgccgt cacatcgctg ccatcatcat ggtagcggta
     8101 atagaagttg cttttgagaa catgcagttc aataagttgt accgggctga acggcggcgg
     8161 catcgggatg aaggcgcgta ataaagacgc cttaccgtgt ttctgtgcta accgctccgc
     8221 gagcattccg tttaccgctt caattgttat tccttgtggc ggggcaaaca tcgcctcgtc
     8281 tgacatagcc atcaacagtc cttattaatg agaaatcctt ctcacgcaat ttttctggcc
     8341 atcagataga tggccggttc ctgcacaatt tcatccaaca gatgaatgaa ttgaatgcta
     8401 cgcgcctgcc acacagacgt cgcgtcatcc gccagtggcg cgaatgcccg ggtcaacagg
     8461 gcggtgtggc tggcgtcaat aacaatctca ccaaaagtca tcacccccag catcttacgt
     8521 cgggcttgct cctcttcgac ctgggcaagc caatcttcgt attccgtcag tgggcagggg
     8581 aaggcgacat tcagacaatc aatcacgcca acgtgatgac cgatagccag cgagtaatac
     8641 attacctgag agctgtgttc cggcatctcg tcgctactgg cgacaaactt ttttcgcagc
     8701 gtccagaaaa caatttcccc gcactcttca gtcatgtaac ccccggagta cgcgaaagag
     8761 ttgctgaacg atactattga gacgtggatc gtcggcatcg cgcaaccagg tgttaacccg
     8821 atttcctgta ggatcgtcgc ttaaatggcg caggagccga tcgcaaattt ctcgcccctg
     8881 acgatagccc gccagccgcc gcgcttctcg ctcaatggcg atacgctgtg atggcgggat
     8941 ctgcggccac aacggttgca tagtcacccc ggtgggatcg cgataatcca ccgcgtgaat
     9001 cttctgttgc agcaaaccga gcgccacggc gaaaccgtga atggtggcgg ccggtgttgg
     9061 cgggcagccg gggatccaaa catcaatggg gacaatggtg tcgctgccgc cccagacgct
     9121 gtagagatcg tggaaaatcc gccgccggca ccgcacgcgc cgtaggaaac acagatttta
     9181 tgatcggggg cagactcata cgcccgaagt gcaggcatac gcatcgcccg ggtgactgcg
     9241 cctgtaaata acaaaatatc ggcgtgacgc ggtgatgaaa taaccttaat gccaaaacgt
     9301 tctgcgtcga atactggtgt gatggcggca aaaatttcga tttcacaggc gttgcagccg
     9361 ccgcaatcga cgcgataaac gtaagccgag cgacggatat cctgtagcaa atgctgcttc
     9421 atcttttgcg tttgctcgtc cagcgtgatg ggctggctga catgttgtgt aagcactgga
     9481 ctcatagctg ctccttagcc accagcggta catctgtatc gtcgttgagc agcgtcgcgc
     9541 gttgtttgca ctccgggcag acgctcgctt gcgcccataa catttcgcgg ttttgtggcg
     9601 cattttgttg ctgtgccaac aattcagcag ccagtgcgac ggttttttgc ggggcaaacg
     9661 ggcgttcgca acggctgcaa cgttgtagat ggaacgtcgc gcgggtatag agatcggctt
     9721 tattggtgac ggtcagttca aagttattgg taagctggat ggctctggtc gggcacactt
     9781 cttcacaacg tccgcagtaa atacaacgcc tcagatagag ctgccaggtg cgcgaatttt
     9841 gctggtcgtc ggtctggata gtcagcgcat ttgccggaca agcacaggcg caggcaccgc
     9901 aggcaataca ttggctgggc atcaggtccg gtttcccgcg aaagcctggg ctgacctcca
     9961 gtggcgcgaa gggatatttc accgtcgcgg ttccggcgcg cataatagtt ttcagtaact
    10021 tcagcatctt ctgtccttac ttcagcggcg aacggttacg atcaataccg tagcgttcga
    10081 tctctttata cggcacggtt tttgactggc gcttgcgcac atctaccagc gtcacacggt
    10141 cggtacagga gtagcaggga tcaaggctac cgataatcag cggtgcgtca gaaacggtat
    10201 tgccgcgcag catgtaacgc aacaccggcc agttggcgta ggtggcggca cggcagcgcc
    10261 agcggaacaa tttttggtta tcaccgagca tgctccagtg cacgtcttca ccgcgtggcg
    10321 cttcaacaaa gcccagcgcg aatgcgtgag gtttatagct aaagccttcg gtcagcagtg
    10381 gggtatccgg catgttgtcg agggcaaatt ccagcattgc cagcgaatca aacgtctctt
    10441 tgacacggac catcacgcgg gagaaaacat cgccgccggt aaaggtaaac agtgtttttg
    10501 gaatattgcc gtagtcggcg taggggtgat caaagcgcaa atcacgggcg atttgtcggt
    10561 cgagaatgcc aatgccctga gtgcgttgtt ccatattcgg cgtagcaagc agcatctcta
    10621 ccagctccga cacgtcggcg cgcatctcgc gcaccagttt cagcgtttgc agacgttgct
    10681 ctttgagaat atcgcggcga acaccaccaa tcagattcag accgtaggtt ttacgcgacc
    10741 cgatcagcaa ttccgccatc gtcatcgact tttcccgcac gcggaaaaat tgcataaagc
    10801 cggtatcaaa accaacgaaa tggcaggaga ggccaaggtt aagcaaatga ctgtgtagcc
    10861 gttcgacttc cagcagaatc gagcgaatag tatgtgctcg ttgcggcacc tcaatcccca
    10921 gtgcattttc aaccgagttg gtataggcca cactgtgggc aaaaccgcaa atcccacaca
    10981 cgcggtccga taagaaggtc acttcgttgt agcccatccg cgtttctgcc agtttctcca
    11041 tgccgcgatg gacataaaac aggcggtaat cagcatcgac aatttgctcg ccatccacaa
    11101 acaagcggaa gtgacccagt tcatcggagg tgatatgcag cgggccgaca gggatcacct
    11161 gcgcatcgct gttgccctca ttgataaacg gatacgtttc ggaatcagtc gtcggttcag
    11221 ggcgcagtcg ataatccatc gcatctttgc gcagcggatg catatcttcc ggccagtcat
    11281 cgggcaacac caggcgacga tgatccggca ggccaaccgg aatcagcccg tacatatcgc
    11341 gaatttctcg ctcgccccag accgcggcag ggacgcgcgg tgtgactgac ggaaactccc
    11401 gactgtcggc atcgaccagt gccttcacca cgatccagca tttttcggcc ccttccattg
    11461 aaagggcata ataaaccgcg taatgaccgt taagtgtccg ctcgtcgttg ccaaacagga
    11521 ctggaagcca gccatcatgt tgataataaa gataatgtac aacgtcaggc agcagattga
    11581 ttttcaccgt aatggtgacc tgttcaggcg tttgtcgctc ttcatccagc accgcgccgg
    11641 ggaactgcgt tttcagggcg gcgagaatcg cttcgccacg atttgacgat gaattaacgt
    11701 tcactgtgtt tctccgaaac agatgcggtg cctgaaggca aaaaatcatg ccaggtgcta
    11761 cgttgtgcag gcaggtcgtg cgtccctgag aggactatag tggaagcgcc cgccaggatc
    11821 ctgatgacag gttgtggaat atgcgttccc atcgccagca tcatgaccag cagaatcacc
    11881 attggcgagg tggtcaacca gccgagatca ccccggttaa cggcctgcgg cggtttcgcc
    11941 attaacaccc gcgctgccat ccgtaccagg cccgccagca ccagcgttaa cagcaataac
    12001 agcaggacga taatcagcag gtgattacgt gccagtccgg cggtaatggt cataaattcg
    12061 ctaagaaaaa tgttgaaggg cggcatccct gccagcgcca gcgcaccgcc gccaaacagc
    12121 acggcggtaa atggcatgat tttgagcatc ccacagacga cgttgagatc gcgcgtgccg
    12181 tacttgagca gtacattgcc ggaaccgcag aacagcagcg tttttgccag actgtggttt
    12241 aaggtgtgca gcagcgcggc aaaaattccc agtggcccgc caatgcctag cgccaccgcg
    12301 accagtccca tgttctccac gctggagtac gccagcagac gcttaatgtc ccgctgtacc
    12361 agaatgaaaa atgccgccac ggcaaccgac aacatgccga agatgagcaa caaccggttg
    12421 gggaaatcgc tgccgatggc ctggcagata atgatgtaat agcgaattag caccaacagc
    12481 gcgcagttca gcaatacggc ggagagcagg gcgctgaccg gactcggtgc ttcactgtga
    12541 gcatccggca gccaggcgtg catgggaaat agcccggttt tggtgccaaa gccaattagc
    12601 acaaacacaa aggccaacag cattaatgtt gggtcaagca aggacgattg cttaagaacc
    12661 tcgctccaga atatcgccat ttctgcctgc ggcataacgc tggcgacgtt ggcgtatacc
    12721 agcacggtac cgaacagacc aaaagcgaca ccaacagtac aaataatgat gtacttccat
    12781 gctgcttcca gcgatgaacg ctgaccgtaa atgcctacca gaaacgccga gcttaaggtg
    12841 gtggcttcga tcgccgccca catcacaatc aggttattgc tggtaacaac cagcagcatg
    12901 gtaaacaaaa acagatggaa gaaaccgtag taatcgcaca gcgtaacggg ggaaagctcg
    12961 ccgtgtgcca cttcatgacg catgtagcca atcgagtaaa caccggtgag aaagccaatc
    13021 acaccaagaa tggcgaggaa caaaccgccc agaccatcaa tatgaagcca cagtcccgca
    13081 gcgaatattt ctcctgcatc agcggcagtt tggaccaccc agagtgccag aatcagcagc
    13141 agtgtgatcc ctaagctatg taatacagtc accgtacaag tcgcagagag cccccgtttc
    13201 cggcaggcaa aacagagcag cgaaaaaagc agcggcgtga gcaggagtaa agcgaacatc
    13261 acagaataac tcatctcatt atcccttcag cgcggtcaag ttgttcacgt ccagcgtgcc
    13321 gtgggtacgc catatttttc ttgccagtaa caccatcaca atgacggcga agatggcgtc
    13381 ggtagcgata cctatttcca ccagttccgg tgctcgccag gcaagaagcg ccagcaccag
    13441 atgggagccg ttttccatca ggcagtaacc aaaaatttgc cgcaggatat tgcgctggct
    13501 gacaatgcac agcaggccaa gcagaaaatg acctaacgct accgccagcg ccggtttcag
    13561 cccggtagcc atcggtagct tcacgggctg aacgacaaat gcgcaaagca ggacaattaa
    13621 cgccgcgagc agtgccatca ttgccggacc gaataacgct ttttccggga tgttctgggg
    13681 aatatttcgt gcagcgtaag tcatgattaa cggtaccagc agcactttgg tgataaaggc
    13741 gctggcggac cagatcagca gttgctctgc ggcgaacagg cacgagagag tggcgaaaat
    13801 agacaccagc accagcgact ggcaggcgta aaatccgcat gacaggcgat agcttttgac
    13861 gctaatcaca aacagcgatg tcagcatcat cagtcccgcc agattattta cgatcataga
    13921 accggtcatt catcatctcc ttactgcaac caggtcgcgg cgattacgct ggaaatcagc
    13981 gacatcacaa tcagtacaat caacaccagg cgcattgacc ctggcagcgg tgcggcatcg
    14041 gcgacggcct cgctcggttt gccaggcaca acgcgaccaa accagcgaat aaaccaggcg
    14101 aaactggcga ccgattcaat catcagcaga atcatggcgg gcagcaggat ccagtactcc
    14161 actgacaacg caaaaccggc agcaaacagc gggaatttac tgaagaagcc gttgaacggc
    14221 ggcacgccgg taatcgccag cgctgccacg cagaaaccca cgcctggcaa cggcagggtg
    14281 tgcaatacgc cacgcagacg cggcaacaag cgcgtgccgc agctgtaact cagcgcacct
    14341 gctacaagga aaaacaggct tttagcgaac gcgtggttga cgatgtaggc gatgctgccc
    14401 tccagcgcca gccgcgagcc gaagatggag agcgacaaac cgaagaacat ccagccaagt
    14461 tgagtgatgg tcgaccaggc cagcaaccgc ttcatatcct gctgtggcaa atacatcaga
    14521 aagccataaa gaatggtgac cagcgccatg accatgccaa cgccgccaat cacatgcggg
    14581 atattgccgc cgtcgataat ggcgcgggca aaaatgtaaa cgcccacttt caccttcgat
    14641 gcggcgtgga gataggcact gatcggtgtt ggcgcttcca ttgcatccgg tagccacgct
    14701 tgcatcggta gctgggccga tttcccccac gcggcaaaca ggatgccgcc ataaaccaga
    14761 taacgtgcgt cgccgtgtaa ctcgctcatc gcgctaagcg caaacgttcc ggtctgcaaa
    14821 aacagcgtgg cggcggcaag atacaacccc aacgagccga tatgagtgat aagtaacgct
    14881 tttagtgctg aacgctgtgc tttatcgctc tggtaataac tgatcaacgc ccaggagcag
    14941 ccgcccgtaa tttcaaaaaa caacaactga ccgagcagcg tcgaggagag taccagtccc
    15001 gccatcgcgc cgataaacaa cagcaaaaat gcgtaataac gattcgtgcc gttatgcggg
    15061 tgttcgcgat ttttatccgt cagataaccc gtggagtaga tcgtgaccag caaaccgaga
    15121 aacaccacca caaacagaat cagcgtactc acgcgatcaa tgaccaggcc aaacaatgcc
    15181 acatccccca cgttgaccaa tgtcaacgtg acggcaactt tatcggcctg ataaaacgcg
    15241 gagatcagtg acaacatgca cagcgtggtc agcgcggcga acaaaacccc ccattcggcg
    15301 gcccgacgtt gtggcgaaaa cgaaacgacc agtgcgccga taaaaggcag caataacgtc
    15361 gtcagagcaa gattttccat attccgtcag tgctccttac agaccggtta accagaagac
    15421 ccatgcaagc gcagcaaggc tgaagccaag ccaggtcaca tggtgaatga gtaaaaaacg
    15481 cccgcgtgcc agcgtgtttt ctgcgattga ggccagtaca aaaatcagca aaaccttgag
    15541 cagcgtaacg acaagtgaag tcagcaggca ggcgagagaa agttcttgcg cgcgcccaaa
    15601 ggacagaaac agggccacaa acagtgatgc catcacgacc tgtttcagcc ccagccccca
    15661 tttcgctagc gccagcccgg caccggaata ttcggtcagc gggccttcct gtaattcctg
    15721 ttctgcttca gcaacatcaa agggaatttt tcccatctca atgaagcagg caaaaccaca
    15781 ggccagtaac gccagtacgg tggttagcgg cgagttccag cccatcgcca gcgtattgct
    15841 gatcatctcg atatgcgtgg aacctgctat cagcgccaat accagcagtg agagaataag
    15901 cattggttcg accagaatgc cgagcgtcaa ctcgcgactg gcaccgactc ccgcaaacgg
    15961 acttccggta tccagcccgg aaagagcaaa gaaaaaacga aacagggcaa gaagatagat
    16021 aagggtgatc agatcgccgc cgcccgcaaa aggggaaacg gtaataaaca gtggtaaggc
    16081 catcgccagc accagcatgc tgctgattaa tacccacggc atcaggcgga acatcagacc
    16141 tgaagatgtc ggcgcaactt cctggcgttt aaacagtttg tggatatcgc gataatcctg
    16201 ccagatcccc ggcccgcggc gggagtgcat acgcgcgcgt atctgccggg aaatacccgt
    16261 aaaaagtggg gttagcatca gcagaatcac ggcctgtgct aacgcaaaaa taaccagata
    16321 tccgtcgcaa agagtttgtc tcatggtgat ttccttagac ggcaatagcg attagcagca
    16381 caaccagtgc ggcgaccaca tacaggcaat agagacgaaa gtcgccgctt tgcagatgct
    16441 gtatttcttt ggccagccgt tgggtggcgc tcacgatggg gcggatcacc cgctcatccc
    16501 agaagggttc tgtgctctga gccctggcgg tgacgtgcgc aaaaccttta tttagcctca
    16561 gcgtagggtc gagctgttgt cgtagacgaa ataacgcaga gaagaccaca cgcagcggct
    16621 gcatcacgcc attgcctgac ggggccatcg cattttccca gccgtagccg catgcccagg
    16681 catctcctgt gcgacgaaac gctgcgcggc gcgaacgaca ccacagccag taaaggccag
    16741 gcagcaaagg catcgccagt agtaacagca acagtaatga gggggtgacc tgtgtatgaa
    16801 acgtgccggg tacaagtgct attccgctgg cgacagtgac gggaggggta ttggtaaacg
    16861 catgggcaat atgcattatc ttcggtgcca gccaactggc actaagcgca attaatacgc
    16921 agagtgccgc gagcagtagc atggcgacga tcatcgtatt tggcacttcc tgtgcctctt
    16981 cagcgtgtgt actgcgcggc gcaccacaga aagtaatacc gtacattttt acgaagcaca
    17041 ttactgccag cccaccagtg actgccagca ttaccatagc aataggaccc gcaagttgta
    17101 gcgctacggc ttccacacga cttagtgaga acagcgactg ccaggtgtac cattcgctga
    17161 taaaaccatt cagcggagga atggctgata tcgcgaggca accaatcagg catgctgcgg
    17221 ctgtccacgg catccgtttc gccagtgccc ccattttttc catgtcgtgg gtatgcaaac
    17281 gcgaaataat cgcgcccgcg ccgagaaata gcagcccttt gaacagcgca tggttgagca
    17341 gatgaaacag tgcgccgagc agtccaacca cggtgagcag cgggtcgtgc agtgacagac
    17401 cgaccatcgc cacaccgact gccagcaaaa taatgccgac gttttcgacg gtactccagg
    17461 ccagcagccg tttgatatcc tgttccgcca gcgcatacag cacgcccagg agcgcggaga
    17521 ttgcgccgat cgccatcacc agaatgcccc accacagcgg caaacccgtt tgcgccagca
    17581 ggtccatcgc tactttcagg atgccgaaaa taccgatttt gaccattacg ccagacatca
    17641 acgccgaagc gtgcgatggt gcggcagggt gagcgcgcgg caaccagctg tgcaacggca
    17701 tcatcccggc tttcgcgcca aaaccgaaaa aggccagcag gaacaccgcc gacgccagcc
    17761 ccggagaaag tgaaagcgtg cggaaactgg caaaatcgag gctgccgctt tcgcgcccca
    17821 tcagcaagaa ggcgatcata atcagcaccg atccggcgtg ggcgataaaa aagtagagca
    17881 tgccagcgtt gatcgacgtt ttatcctgcc tggcaatgac cagaaaccag gaagacagcg
    17941 acatcatttc aaacagcacg atgaaccaaa aagcgttgtc catcaccagc agggcaacca
    18001 tcgatgcgat gaaaagattc ataaagaagc ccatcgccgc cgcgcctttg ccctcgtatt
    18061 cgcgcatata agtcaatgaa tagagcgaac aaaccaccac cagcaaggag ataaccagca
    18121 ccataaatgc cgagaggcta tccattcgca gggtaaaatc ggcaaagtca aaaggggtgg
    18181 caaaacgggc gactaaagtt acaccagcat gtaattgcgt aattccgctg atgatgccaa
    18241 tcacgccgcc caccagcgat gtgatgccgg aaagcttaat agccagtctg tccagaccga
    18301 gtaaaaacag cgaagccaga ctggcaaaca gatagaggat cagcgaccag gttaataatt
    18361 gcagggcatc cattagcgtt gctcctgagt gagggaaagg ggatcctccc cgccattggc
    18421 aaaccagctt gcggcaaggc gctgtttttc tttcatctgt cgttgcaggc tatcgtcggt
    18481 gatcagccgt aacgcctgat tcgggcaagc gcgaacgcag gccggacctt ctggcaagaa
    18541 atcacacagg tcgcatttca ccgcgacggt ctggacaccc ggttcccagc gcagcaatgc
    18601 atgttggggc ggcacgtttt cttcgccgtc tttgagtgag ccttcagcct gaaaaacata
    18661 ttgcgcaggg gcattcaccg gacggcttcc tgaagcgctg attgcgccaa atgggcagac
    18721 cacggcgcaa agcttgcagc caatacagag gctttcgttg agttggatcg catcatccct
    18781 ctgggagatg gcattaaccg ggcagacctg caggcaaggg gcttcctcac agtgatgaca
    18841 cacgacaggg gcagtgagtg ttgacgtctt cgccagggcc aggcgcgggt gttgctgtaa
    18901 accttgcgtt ttatgcacgt ccgaacaggc agcgagacag gtattacatc ctgtacacca
    18961 cagtggttcg gccaccacaa agcggttcat gtcggctcct tgaaaatgat atgaaacatt
    19021 gcatttcata ttgcataaag gatgccattc tgattaactc aatatattca ataagttatg
    19081 tattgactgg attctgaacg gtacaaaaat gatggtcatt aacgttcagc aaaaaatgga
    19141 ttcgacaccc gtgtcgcagg ggaaagcgag aatgtgatct tgatcacgag aagtgaggta
    19201 tgctggccgc aagcgcagcc agcacggaat ggagcaaagt aatcaggcgt gttctttcag
    19261 ccagttcagc acaacgtcgt ggtgattgct ggttttgaaa tcgtcaaaga catgttcgat
    19321 tttgccatca gcgtcaatca ggaagctgat gcgatgaatg ccatcgtagg ttttgcccat
    19381 gaaggacttt tcaccccaga cgccgaattg ttcgcacacc tggtggtcct catcagacag
    19441 gagcgtaaag ttaagcagct ctttttccgc aaaacgggag agtttttcgg gtttatcggt
    19501 gctgataccc agcacatcaa cgcccgcttt tttcaactca tccatgttat cgcgtaagcc
    19561 gcaggcctgt acggtacagc cgggggtcat ggctttcggg tagaaataaa ccagaacacg
    19621 ctgtccctgg aagtcggtca aattaacttg ttctccgtct tgatccggca agctaaattt
    19681 cggtgcgata tcaccggctt tcagtggatt cattacttaa ctccatcctg ttcatcatgt
    19741 tgggaataat tgacgacgtt aatactgcct tgtgcattga gttctgtaca tagggctttg
    19801 aacgcttgct caatatttgc tgcgtccgca gatgcggggc tgtgggcggt tatctgaata
    19861 tgcaactgcg cagcccgttc attttcagca ggttgcgtgc gcgacaccag ctccgcaatg
    19921 ttcatatgat gcgcgtcgaa aagtgctgtg aagcgttcaa ttaaatgcgg ggagtctgcc
    19981 acatcgacct gaacccagac agatgctggc attggcggac gcggacgcgc cgtcgtgcgc
    20041 ttcatcacga ttaaaagatc cagttcggca cctttcaacg gtaacgttga ttcaatcaga
    20101 gtgatggcat tccatgaacc ggaaagcagc atgataaacg tgaactcttc tcccagcatc
    20161 gccaggcgac tgtcttcaat attacagccg caactactga catgacgggt gatggtgttc
    20221 acaattccag ggcgatcggc acccaacgca gtgatcacca gataatgttg cgatgacagt
    20281 gtcaaactgg ttattccttt aaggggtgag ttgttcttaa ggaaagcata aaaaaaacat
    20341 gcatacaaca atcagaacgg ttctgtctgc ttgcttttaa tgccatacca aacgtaccat
    20401 tgagacactt gtttgcacag aggatggccc atgttcacgg gaagtattgt cgcgattgtt
    20461 actccgatgg atgaaaaagg taatgtctgt cgggctagct tgaaaaaact gattgattat
    20521 catgtcgcca gcggtacttc ggcgatcgtt tctgttggca ccactggcga gtccgctacc
    20581 ttaaatcatg acgaacatgc tgatgtggtg atgatgacgc tggatctggc tgatgggcgc
    20641 attccggtaa ttgccgggac cggcgctaac gctactgcgg aagccattag cctgacgcag
    20701 cgcttcaatg acagtggtat cgtcggctgc ctgacggtaa ccccttacta caatcgtccg
    20761 tcgcaagaag gtttgtatca gcatttcaaa gccatcgctg agcatactga cctgccgcaa
    20821 attctgtata atgtgccgtc ccgtactggc tgcgatctgc tcccggaaac ggtgggccgt
    20881 ctggcgaaag taaaaaatat tatcggaatc aaagaggcaa cagggaactt aacgcgtgta
    20941 aaccagatca aagagctggt ttcagatgat tttgttctgc tgagcggcga tgatgcgagc
    21001 gcgctggact tcatgcaatt gggcggtcat ggggttattt ccgttacggc taacgtcgca
    21061 gcgcgtgata tggcccagat gtgcaaactg gcagcagaag ggcattttgc cgaggcacgc
    21121 gttattaatc agcgtctgat gccattacac aacaaactat ttgtcgaacc caatccaatc
    21181 ccggtgaaat gggcatgtaa ggaactgggt cttgtggcga ccgatacgct gcgcctgcca
    21241 atgacaccaa tcaccgacag tggtcgtgag acggtcagag cggcgcttaa gcatgccggt
    21301 ttgctgtaaa gtttagggag atttgatggc ttactctgtt caaaagtcgc gcctggcaaa
    21361 ggttgcgggt gtttcgcttg ttttattact cgctgcctgt agttctgact cacgctataa
    21421 gcgtcaggtc agtggtgatg aagcctacct ggaagcggca ccgcttgcgg agcttcatgc
    21481 cccggctgga atgattttgc cggtgacctc cggtgattat gcaatcccgg tgaccaacgg
    21541 tagtggtgct gtcggtaagg cgctggacat tcgtccacca gcccagccgc tggcactggt
    21601 ttctggcgcg cgtacccagt tcacgggcga taccgcttca ttgctggtgg aaaatggtcg
    21661 tggcaatact ctgtggccgc aggtggttag cgtgctgcag gcgaaaaact acaccatcac
    21721 ccaacgtgat gatgctggtc agacactgac caccgattgg gtacaatgga accgtctgga
    21781 cgaagacgag cagtatcgtg gtcgttatca aatctctgtt aagccgcagg gttatcagca
    21841 ggcggttacg gttaaactgc tgaacctgga acaggcgggc aaaccggttg cagacgcggc
    21901 ttccatgcag cgttacagca cggagatgat gaacgttatt tccgccggtc tggataaatc
    21961 tgccactgac gccgcgaacg ctgcgcaaaa tcgtgcctcc accactatgg acgtacaaag
    22021 tgcagctgat gacaccggtt taccaatgct ggtcgtacgc gggccgttca atgtggtttg
    22081 gcaacgtctg ccagcggcgc tggaaaaagt gggcatgaaa gtgaccgaca gcacccgttc
    22141 gcagggcaac atggccgtaa cttataagcc gctgtctgac agcgactggc aggaactggg
    22201 cgcgagcgat ccaggcctgg catccggtga ctataaactg caggttggcg atttagataa
    22261 ccgcagcagc ctacagttca tcgatccgaa aggtcatact ctgactcaga gtcagaacga
    22321 cgcgctggta gctgtcttcc aggctgcgtt tagcaagtaa aaatacaggg ctggaatcat
    22381 ccggcccttt tttctgatat gatacgcaaa cgtgtgcgtc ggcaggaaaa cgcgatttta
    22441 gcggtaattc gcacgaaatt tgtttgtcgg acgtagttcg gataaggcgt tcacgccgca
    22501 tccgacaaaa catccggcac accagacagc aaaagatttt aaaacgttaa ttcacaccca
    22561 ggagtgataa agatgcaaaa gcaagctgag ttgtatcgtg gtaaagcgaa aaccgtatac
    22621 agcacggaaa acccggacct gttggtgctc gaattccgca atgatacgtc agcaggggat
    22681 ggcgcgcgca ttgagcagtt tgatcgcaaa ggtatggtga acaacaagtt caattacttc
    22741 attatgagca aactggctga agcgggtatc ccgactcaaa tggagcgtct gctctccgat
    22801 accgaatgtc tggtgaaaaa gctggatatg gtgccggttg agtgtgtcgt gcgtaaccgt
    22861 gctgctggct ctctggtgaa acgtcttgga atcgaagaag gtattgagct gaacccgccg
    22921 ctgttcgatc tgttcctgaa aaacgacgcc atgcacgatc cgatggtcaa cgaatcttac
    22981 tgcgaaacct ttggctgggt gagcaaagag aacctggcgc gtatgaaaga gctgacctac
    23041 aaagcgaacg acgtgctgaa aaaactgttc gatgatgctg gtctgattct ggtcgacttc
    23101 aagctggagt ttggcctgta caaaggtgaa gtggtactgg gcgatgagtt ctccccggac
    23161 ggcagccgcc tgtgggacaa agaaacactg gagaaaatgg acaaagaccg tttccgccag
    23221 agcctcggtg gcctgatcga agcctatgaa gccgtcgccc gccgcctggg tgtacagctg
    23281 gactgatttt tctggttgct tgtttcttta gccggagggt actccggcta aatgtctttg
    23341 atacccccgc tacaatactt ctcattatct ccctttgaca ggaaagtcat accaggccaa
    23401 tatgtatata cataacgtat atcccaaaaa gggggggcta tggaaactac tgtattttta
    23461 agtaatcgca gtcaggccgt cagattaccg aaagcggtcg ctctgccaga agacgttaag
    23521 aaagtagaaa ttattgcgat tggtcgtaca agaatcatca ctcccgccgg tgagtcctgg
    23581 gacagttggt ttgatggcga aaatgtgagt gcggatttta tggatatccg tgatcaaccc
    23641 gcaatgcagg aaagggaatc attttgatgc tgaaattcat gctggatacc aatatctgta
    23701 ttttcactat taagaataaa ccagtacatg tcagagagcg gtttaacctg aattctggcc
    23761 ggatgtgtat aagttctgta acattgatgg agcttatcta tggggcagaa aaaagtcaga
    23821 tgccagaacg caatttggcg gttattgaag gtttcgtatc tcgtttggta gtgcttgatt
    23881 atgatagtgc tgctgcgacg cataccgggc aaatcagggc cgagcttgcc cggcagggaa
    23941 ggcctgttgg accttttgac cagatgatag cgggacatgc tcgcagtcgc ggtttgattg
    24001 tggttaccaa caatacccga gagtttgagc gagtggcggg aatcagacta gaagactgga
    24061 gctaacattc tcttttactt ctaacattcg taaattatat aacgtggcat gaagccttaa
    24121 ttcctccgcc atttcatccc gctttcctgt ggtaatcttc ctctgaacca cctacgatca
    24181 tttctataat gaatatattg ttgaggtatc tatgcgttgg caagggcgac gtgaaagtga
    24241 caatgttgaa gacaggcgca acagctccgg tggtccgtct atgggcggtc ccggttttcg
    24301 cctgccaagc ggtaaaggcg ggttgatttt actgttagtc gtgctggttg caggctacta
    24361 tggtgttgat ttaaccgggt taatgactgg acagccggtt tcccaacaac aatcaacgcg
    24421 gtccattagc cctaatgaag acgaagctgc aaaattcacc tcggtgattc tggcaaccac
    24481 ggaagacacc tggggacaac agttcgagaa gatgggtaag acctatcagc aaccgaagct
    24541 ggtcatgtac cgtggaatga cgcgcaccgg ctgcggggct ggtcagtcca ttatggggcc
    24601 gttctattgc ccggcggatg gcacagttta tatcgatctc tccttctatg atgacatgaa
    24661 agacaaactt ggcgcggatg gcgattttgc ccaggggtac gttatcgccc atgaagtcgg
    24721 tcatcatgtg cagaaactgt taggcatcga gccgaaagtt cgtcaactgc aacaaaacgc
    24781 gacgcaggcg gaagtgaacc gcttatctgt gcgtatggaa ctccaggccg actgttttgc
    24841 cggtgtctgg gggcatagta tgcagcagca aggcgttctg gaaaccggcg atctggaaga
    24901 ggcgctgaac gcggcgcagg ccatcggcga tgaccgttta caacagcaaa gtcaggggcg
    24961 agtagtacca gatagtttca cacatggcac ttctcagcaa cgctacagct ggtttaaacg
    25021 tggtttcgac agcggcgatc cggcacaatg caatactttt ggtaaaagca tttaacttcc
    25081 ggggcaggga tggctgaact gactgcgctt cacacattaa cagcgcaaat gaaacgtgaa
    25141 gggatccgcc gcttgctggt gttgagcggg gaagagggtt ggtgttttga tcatgcgctt
    25201 aagttgcgtg atgccttacc tggcgactgg ctgtggattt cgccgcagcc agatgctgaa
    25261 aaccactgtt caccctcggc gctacaaact ttacttgggc gcgagttccg gcatgcggta
    25321 ttcgacgccc gccacggctt tgatgccgct gcctttgcag cacttagcgg aacgttgaaa
    25381 gcgggaagct ggctggtttt gttactccct gtatgggaag agtgggaaaa ccaacctgat
    25441 gccgactcgc tgcgctggag tgattgccct gaccctattg cgacgccgca ttttgtccag
    25501 catctcaaac gcgtacttac ggcggataac gacgctatcc tctggcggca aaaccagccg
    25561 ttctcgttgg cgcattttac tccccgtact gactggcacc ccgcgaccgg cgcaccacaa
    25621 ccagaacaac agcaactctt acagcagcta ctgaccatgc ctccgggcgt ggcagcggta
    25681 acggctgcgc gtgggcgcgg taagtcggcg ttggcagggc aactcatttc tcgtattgcg
    25741 ggcagtgcga ttgtcaccgc gcccgcaaaa gcggcaacgg atgtactggc acaatttgcg
    25801 ggcgagaagt ttcgctttat tgcaccggat gccttgttag ccagcgatga gcaagccgac
    25861 tggctggtgg tcgacgaagc cgcagccata cctgcgccgt tgttgcatca actggtatcg
    25921 cgttttcctc gaacgttgtt aaccactacg gtgcagggct acgaaggcac cggacgtggt
    25981 tttttgctga aattttgcgc tcgctttccg catttacacc gttttgaact gcaacagccg
    26041 atccgctggg cacagggatg cccgctggaa aaaatggtta gtgaggcact ggtttttgac
    26101 gatgaaaact tcacccatac accacaaggc aatatcgtca tttccgcatt tgaacaaacg
    26161 ttatggcgaa gcgagccaga aacgccgtta aaggtttatc agttattgtc tggtgcgcac
    26221 taccggactt cgccgctgga tttacgccgg atgatggatg caccagggca acatttttta
    26281 caggcggctg gcgaaaacga gattgccgga gcgctgtggc tggtggatga ggggggatta
    26341 tctcaacaac tcagtcaggc ggtatgggca ggttttcgtc gcccgcgggg taatctggtg
    26401 gcccagtcgc tggcggcgca cggtagcaat ccactggcag cgacattgcg tggacggcgg
    26461 gtcagccgga tagcagttca tccggcgcgt cagcgggaag gcacagggca gcaacttatt
    26521 gctggtgctt tgcaatatac gcgtgacctc gactatcttt cggtgagttt tggttacacc
    26581 ggggagttat ggcgtttctg gcaccgctgc ggttttgtgc tggtgcggat gggtaatcat
    26641 cgtgaagcca gcagcggttg ctatacggcg atggcgctgt taccgatgag taatgcgggt
    26701 aaacaactgg ctgaacgtga gcattaccgt ttacgtcgcg atgcgcaagc tctcgcaaag
    26761 tggaatggcg aaacgcttcc tgttgatcca ctaaacgatg ccgtcctttc tgacgacgac
    26821 tggcttgaac tggccggttt tgctttcgct catcgtccgc tattaacatc gttaggttgc
    26881 ttattgcgtc tgctacaaac cagtgaactg gcattaccgg cgctgcgtgg gcgtttacag
    26941 aaaaacgtca gcgacgcgca gttatgtacc acacttaaac tttcaggccg caagatgtta
    27001 ctggtccgtc agcgggaaga ggccgcgcag gcgctgttcg cacttaatga tgttcgcact
    27061 gagcgtctgc gcgatcgcat aacgcaatgg caattttttc actgactcct tcaattttct
    27121 cccatggtca ttgtgagtca gcggcgtaaa attaatgaca tcagttaagg agttcgccat
    27181 gaaacatgac cattttgttg ttcaaagccc ggataaacct gcgcaacagt tgttactgct
    27241 tttccacggt gtcggggata acccggtggc aatgggggaa ataggtagct ggtttgcacc
    27301 gctgtttcct gatgcgctgg tggtgagtgt cggtggcgcg gagccgagcg gtaatccggc
    27361 ggggcgtcag tggttttcag tgcagggtat tacggaagat aatcgccagg cgcgtgtgga
    27421 tgccatcatg ccgacgttta ttgaaacggt acgctactgg cagaaacaga gcggagtagg
    27481 ggctaacgcc acggcgctaa ttggtttctc acaaggcgcg attatggcgc tggagagcat
    27541 taaagccgaa ccgggccttg cttcgcgcgt cattgccttt aacggacgct atgcgagcct
    27601 gccggagacc gcgtctacag ccacgacaat tcacttgatt catggtggtg aagatccggt
    27661 tatcgatctg gcgcatgcag ttgcggcgca ggaagcgtta atcagtgccg gtggtgacgt
    27721 gacgctggat atcgtggagg atttaggcca tgcgatcgac aatcgcagta tgcaatttgc
    27781 cctggatcac ttgcgttata ccattccgaa acattatttc gacgaagcgc taagtggcgg
    27841 taagcctggc gatgatgatg tgattgaaat gatgtaaaag acagattact tggcctgata
    27901 agacgcgtga gcgtcgcatc aggcatcgtt caccattgcc ggatgcggcg ttaacgcctt
    27961 atccggccta cggttgagca ctcagcttac ttcttcggtt gatccttttt cggccagtcg
    28021 tcgtcatcgt cccacttatc gttgaaatca cgatgcgggg gaagctccgg cttgttggcg
    28081 agaaatttct tatggtcgac gcgcttgaga tctttaatca cgttcagcag gacgcctacc
    28141 aaaaagacaa tcaccagaat ccaccaatat ttagccagcc agtccatgct tatttcctct
    28201 tgcagaacca ctcatcaggc gacgagctgt tccatgatac gttgatacat acgggcaagt
    28261 agctgcaggt tggcagcgtt cacacattca ttaattttat gaatagtggc attgaccggc
    28321 ccgagttcca ccacctgcgc ccccatgcga gcaataaagc gcccgtcgga cgttccgcct
    28381 gtggtcagta gctgcggttt aatttcatta tagtgctcaa ccgcgttaac gaccgcatcc
    28441 accagtttac cgcgcgcggt caaaaatggc tgcccggaaa gccaccagtc caccgtataa
    28501 cgcagttgat gtttttcaag cagggcaagc acctgtgctt tgatcatctc atcagtcagt
    28561 tcggtgctga agcggaagtt aaactgcaca aacagttcac ccggaataac gttgttactg
    28621 cccgttcccg cctgaatatt ggcaatctgc atactggtcg ccgggaagaa ttcattgccc
    28681 tgatcccact caatagccac taattcatta aggaaaggtg ctgcgcgatg taccggattg
    28741 tcagccagat gcgggtaggc aacatgcccc tgaacgccat gaatggtgag gttgcaggtt
    28801 aacgatccgc gacgaccatt tttcaccaca tcacctacca cttcgatact cgacggttcg
    28861 ccgaccaggc agtaatcgag acgctcatta cgtgccatta acgcttcgac gacttttacc
    28921 gtaccgttgt gtgcactggc ttcttcatca gaggtgatca gaaatgccag tcgccccgta
    28981 tggttgggat gttgtgcgac aaagcgttct gccgctacca ccatcgccgc cagcgagcct
    29041 ttcatatctg ccgcaccgcg cccgaataac atgccatcac gaatggtggg ttcaaacggc
    29101 ggattgatcc aacgatcggc gtcgccaggc ggcaccacgt cggtatgccc ggcaaaggct
    29161 aacgtttcac cttgcccacg ccatgcccaa aaattctgcg tatcggcaaa gtccatgcgt
    29221 tcaacggtaa aaccgatcgc ctgcaaacgt tcaatcaaca aagcctggca tcctgcgtca
    29281 tcaggactca gggaagggcg gcgaataagc tgttgtgtca gctcaataac cgggcacgac
    29341 atagactaca cctcatggaa aaattgctga taactggaat cactgaaacc cagcagcata
    29401 ggcttaccag gcgcgcagag caatggacgt ttgataattg caggcatttc agtcattaat
    29461 gccgccgcag aggccgcatc ggtgatttta ttgcgggtgg tttcgtccag tttacgccag
    29521 gttgtaccac gggtgttgag taacgcttcc cagcctaatt cgttgataaa accgttcaat
    29581 aattcgctgt ccagcccatc gacgcggtaa tcatgaaaac gatagtcgat gttattggct
    29641 tccagccaac ggcgagcctt tttaatggtg tcacaatttt tgatgccgta aagtgtaacc
    29701 ataatgaatc ctttttcgat ttatgagaat aaatactcat ttaagggtga aaa
//
LOCUS       DAZKHF020000030        29616 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2201S31241
            SAMN43267800-rid25106413.denovo.030, whole genome shotgun sequence.
ACCESSION   DAZKHF020000030 DAZKHF020000000
VERSION     DAZKHF020000030.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267800
            Sequence Read Archive: SRR30307263
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 29616)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 29616)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 29616)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350581.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350581.2
            Genome Coverage       :: 151x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:21:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,652
            CDSs (total)                      :: 5,557
            Genes (coding)                    :: 5,320
            CDSs (with protein)               :: 5,320
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 237
            CDSs (without protein)            :: 237
            Pseudo Genes (ambiguous residues) :: 0 of 237
            Pseudo Genes (frameshifted)       :: 102 of 237
            Pseudo Genes (incomplete)         :: 130 of 237
            Pseudo Genes (internal stop)      :: 52 of 237
            Pseudo Genes (multiple problems)  :: 41 of 237
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..29616
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267800-rid25106413.denovo.030"
                     /serotype="O 26:H11"
                     /isolate="2201S31241"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-01"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            complement(131..778)
                     /gene="gloC"
                     /locus_tag="ACHOSC_RS05470"
                     /old_locus_tag="pgaptmp_001094"
     CDS             complement(131..778)
                     /gene="gloC"
                     /locus_tag="ACHOSC_RS05470"
                     /old_locus_tag="pgaptmp_001094"
                     /EC_number="3.1.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003035823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyacylglutathione hydrolase GloC"
                     /protein_id="HGU4547073.1"
                     /translation="MNYRIIPVTAFSQNCSLIWCEQTRLAALVDPGGDAEKIKQEVDD
                     SGLTLMQILLTHGHLDHVGAAAELAQHYGVPVFGPEKEDEFWLQGLPAQSRMFGLEEC
                     QPLTPDRWLNEGDTISIGNVTLQVLHCPGHTPGHVVFFDDRAKLLISGDVIFKGGVGR
                     SDFPRGDHNQLISSIKDKLLPLGDDVTFIPGHGPLSTLGYERLHNPFLQDEMPVW"
     gene            complement(805..1353)
                     /locus_tag="ACHOSC_RS05475"
                     /old_locus_tag="pgaptmp_001095"
     CDS             complement(805..1353)
                     /locus_tag="ACHOSC_RS05475"
                     /old_locus_tag="pgaptmp_001095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF882 domain-containing protein"
                     /protein_id="HGU4547074.1"
                     /translation="MDKFDANRRKLLALGGVALGAAILPTPAFATLSTPRPRILTLNN
                     LHTGESIKAEFFDGRGYIQEELAKLNHFFRDYRANKIKSIDPGLFDQLYRLQGLLGTR
                     KPVQLISGYRSIDTNNELRARSRGVAKKSYHTKGQAMDFHIEGIALSNIRKAALSMRA
                     GGVGYYPRSNFVHIDTGPARHW"
     gene            complement(1534..3381)
                     /gene="ldtD"
                     /locus_tag="ACHOSC_RS05480"
                     /old_locus_tag="pgaptmp_001096"
     CDS             complement(1534..3381)
                     /gene="ldtD"
                     /locus_tag="ACHOSC_RS05480"
                     /old_locus_tag="pgaptmp_001096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase"
                     /protein_id="HGU4547075.1"
                     /translation="MLLNMMCGRRLSAISLCLAVTFAPLFNAQADEPEVIPGDSPVAV
                     SEQGEALPQAQATAIMAGIQPLPEGAAEKARTQIESQLPAGYKPVYLNQLQLLYAARD
                     MQPMWENRDAVKAFQQQLAEVAIAGFQPQFNKWVELLTDPGVNGMARDVVLSDAMMGY
                     LHFIANIPVKGTRWLYSSKPYALATPPLSVINQWQLALDKGQLPTFVAGLAPQHPQYA
                     AMHESLLALLSDTKPWPQLTGKATLRPGQWSNDVPALREILQRTGMLDGGPKITLPGD
                     DTPTDAVVSPSAVTVETAETKPMDKQTTSRSKPAPAVRAAYDNELVEAVKRFQAWQGL
                     GADGAIGPATRDWLNVTPAQRAGVLALNIQRLRLLPTELSTGIMVNIPAYSLVYYQNG
                     NQVLDSRVIVGRPDRKTPMMSSALNNVVVNPPWNVPPTLARKDILPKVRNDPGYLESH
                     GYTVMRGWNSREAIDPWQVDWSTITASNLPFRFQQAPGPRNSLGRYKFNMPSSEAIYL
                     HDTPNHNLFKRDTRALSSGCVRVNKASDLANMLLQDAGWNDKRISDALKQGDTRYVNI
                     RQSIPVNLYYLTAFVGADGRTQYRTDIYNYDLPARSSSQIVSKAEQLIR"
     gene            complement(3642..8102)
                     /gene="mukB"
                     /locus_tag="ACHOSC_RS05485"
                     /old_locus_tag="pgaptmp_001097"
     CDS             complement(3642..8102)
                     /gene="mukB"
                     /locus_tag="ACHOSC_RS05485"
                     /old_locus_tag="pgaptmp_001097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA];
                     GO_process: GO:0030261 - chromosome condensation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromosome partition protein MukB"
                     /protein_id="HGU4547076.1"
                     /translation="MIERGKFRSLTLINWNGFFARTFDLDELVTTLSGGNGAGKSTTM
                     AAFVTALIPDLTLLHFRNTTEAGATSGSRDKGLHGKLKAGVCYSMLDTINSRHQRVVV
                     GVRLQQVAGRDRKVDIKPFAIQGLPMSVQPTQLVTETLNERQARVLPLNELKDKLEAM
                     EGVQFKQFNSITDYHSLMFDLGIIARRLRSASDRSKFYRLIEASLYGGISSAITRSLR
                     DYLLPENSGVRKAFQDMEAALRENRMTLEAIRVTQSDRDLFKHLISEATNYVAADYMR
                     HANERRVHLDKALEFRRELHTSRQQLAAEQYKHVDMARELAEHNGAEGDLEADYQAAS
                     DHLNLVQTALRQQEKIERYEADLDELQIRLEEQNEVVAEAIERQEENEARAEAAELEV
                     DELKSQLANYQQALDVQQTRAIQYNQAIAALNRAKELCHLPDLTADSAAEWLETFQAK
                     ELEATEKMLSLEQKMSMAQTAHSQFEQAYQLVVAINGPLARNEAWDVARELLREGVDQ
                     RHLAEQVQPLRMRLSELEQRLREQQEAERLLADFCKRQGKNFDIDELEALHQELEARI
                     ASLSDSVSNAREERMALRQEQEQLQSRIQSLMQRAPVWLAAQNSLNQLSEQCGEEFTS
                     SQDVTEYLQQLLEREREAIVERDEVGARKNAVDEEIERLSQPGGSEDQRLNALAERFG
                     GVLLSEIYDDVSLEDAPYFSALYGPSRHAIVVPDLSQITEHLEGLTNCPEDLYLIEGD
                     PQSFDDSVFSVDELEKAVVVKIADRQWRYSRFPEVPLFGRAARESRIESLHAEREVLS
                     ERFATLSFDVQKTQRLHQAFSRFIGSHLAVAFESDPEAEIRQLNSRRVELERALSNHE
                     NDNQQQRIQFEQAKEGVTALNRILPRLNLLADDSLADRVDEIRERLDEAQEAARFVQQ
                     FGNQLAKLEPIVSVLQSDPEQFEQLKEDYAYSQQMQRDARQQAFALTEVVQRRAHFSY
                     SDSAEMLSGNSDLNEKLRERLEQAEAERTRAREALRGHAAQLSQYNQVLASLKSSYDT
                     KKELLNDLQRELQDIGVRADSGAEERARIRRDELHAQLSNNRSRRNQLEKALTFCEAE
                     MDNLTRKLRKLERDYFEMREQVVTAKAGWCAVMRMVKDNGVERRLHRRELAYLSADDL
                     RSMSDKALGALRLAVADNEHLRDVLRMSEDPKRPERKIQFFVAVYQHLRERIRQDIIR
                     TDDPVEAIEQMEIELSRLTEELTSREQKLAISSRSVANIIRKTIQREQNRIRMLNQGL
                     QNVSFGQVNSVRLNVNVRETHAMLLDVLSEQHEQHQDLFNSNRLTFSEALAKLYQRLN
                     PQIDMGQRTPQTIGEELLDYRNYLEMEVEVNRGSDGWLRAESGALSTGEAIGTGMSIL
                     VMVVQSWEDESRRLRGKDISPCRLLFLDEAARLDARSIATLFELCERLQMQLIIAAPE
                     NISPEKGTTYKLVRKVFQNTEHVHVVGLRGFAPQLPETLPGSDEAPSQAS"
     gene            complement(8102..8806)
                     /gene="mukE"
                     /locus_tag="ACHOSC_RS05490"
                     /old_locus_tag="pgaptmp_001098"
     CDS             complement(8102..8806)
                     /gene="mukE"
                     /locus_tag="ACHOSC_RS05490"
                     /old_locus_tag="pgaptmp_001098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415443.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA];
                     GO_process: GO:0030261 - chromosome condensation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromosome partition protein MukE"
                     /protein_id="HGU4547077.1"
                     /translation="MSSTNIEQVMPVKLAQALANPLFPALDSALRSGRHIGLDELDNH
                     AFLMDFQEYLEEFYARYNVELIRAPEGFFYLRPRSTTLIPRSVLSELDMMVGKILCYL
                     YLSPERLANEGIFTQQELYDELLTLADEAKLLKLVNNRSTGSDVDRQKLQEKVRSSLN
                     RLRRLGMVWFMGHDSSKFRITESVFRFGADVRAGDDPREAQRRLIRDGEAMPIENHLQ
                     LNDETEENQPDSGEEE"
     gene            complement(8787..10109)
                     /gene="mukF"
                     /locus_tag="ACHOSC_RS05495"
                     /old_locus_tag="pgaptmp_001099"
     CDS             complement(8787..10109)
                     /gene="mukF"
                     /locus_tag="ACHOSC_RS05495"
                     /old_locus_tag="pgaptmp_001099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005509 - calcium ion binding [Evidence
                     IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromosome partition protein MukF"
                     /protein_id="HGU4547078.1"
                     /translation="MSEFSQTVPELVAWARKNDFSISLPVDRLSFLLAVATLNGERLD
                     GEMSEGELVDAFRHVSDAFEQTSETIGVRANNAINDMVRQRLLNRFTSEQAEGNAIYR
                     LTPLGIGITDYYIRQREFSTLRLSMQLSIVAGELKRAADAAEEGGDEFHWHRNVYAPL
                     KYSVAEIFDSIDLTQRLMDEQQQQVKDDIAQLLNKDWRAAISSCELLLSETSGTLREL
                     QDTLEAAGDKLQANLLRIQDATMTHDDLHFVDRLVFDLQSKLDRIISWGQQSIDLWIG
                     YDRHVHKFIRTAIDMDKNRVFAQRLRQSVQTYFDEPWALTYANADRLLDMRDEEMALR
                     DEEVTGELPEDLEYEEFNEIREQLAAIIEEQLAVYKTRQVPLDLGLVVREYLSQYPRA
                     RHFDVARIVIDQAVRLGVAQADFTGLPAKWQPINDYGAKVQAHVIDKY"
     gene            complement(10106..10891)
                     /gene="cmoM"
                     /locus_tag="ACHOSC_RS05500"
                     /old_locus_tag="pgaptmp_001100"
     CDS             complement(10106..10891)
                     /gene="cmoM"
                     /locus_tag="ACHOSC_RS05500"
                     /old_locus_tag="pgaptmp_001100"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine 5-oxyacetic acid(34)
                     methyltransferase CmoM"
                     /protein_id="HGU4547079.1"
                     /translation="MQDRNFDDIAEKFSRNIYGTTKGQLRQAILWQDLDRVLAEMGPQ
                     KLRVLDAGGGEGQTAIKMAERGHQVILCDLSAQMIDRAKQAAEAKGVSDNMQFIHCAA
                     QDVASHLETPVDLILFHAVLEWVADPRSVLQTLWSVLRPGGVLSLMFYNAHGLLMHNM
                     VAGNFDYVQAGMPKKKKRTLSPDYPRDPAQVYLWLEEAGWQIMGKTGVRVFHDYLREK
                     HQQRDCYEALLELETRYCRQEPYITLGRYIHVTARKPQSKDKV"
     gene            11027..11806
                     /gene="elyC"
                     /locus_tag="ACHOSC_RS05505"
                     /old_locus_tag="pgaptmp_001101"
     CDS             11027..11806
                     /gene="elyC"
                     /locus_tag="ACHOSC_RS05505"
                     /old_locus_tag="pgaptmp_001101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706838.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="envelope biogenesis factor ElyC"
                     /protein_id="HGU4547080.1"
                     /translation="MLFTLKKVIGNMLLPLPLMLLIIGAGLALLWFSRFQKTGKIFIS
                     IGWLALLLLSLQPVADRLLRPIESTYPTWNNSQKVDYIVVLGDGYTWNPQWAPSSNLI
                     NNSLPRLNEGIRLWRENPGSKLIFTGGVAKTNTVSTAEVGARVAQSLGVPREQIITLD
                     LPKDTEEEAAAVKQAIGDAPFLLVTSASHLPRAMIFFQQEGLNPLPAPANQLAIDSPL
                     NPWERAIPSPVWLMHSDRVGYETLGRIWQWLKGPSGEPRQE"
     gene            complement(11783..12676)
                     /locus_tag="ACHOSC_RS05510"
                     /old_locus_tag="pgaptmp_001102"
     CDS             complement(11783..12676)
                     /locus_tag="ACHOSC_RS05510"
                     /old_locus_tag="pgaptmp_001102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcbJ family phosphotransferase"
                     /protein_id="HGU4547081.1"
                     /translation="MEQLRAELSHLLGEKLSRIECVNEKADTALWALYDSQGNPMPLM
                     ARSFSTPGKARQLAWKTTMLARSGTVRMPTIYGVMTHEEHPGPDVLLLERMRGVSVEA
                     PARTPERWEQLKDQIVEALLAWHRQDSRGCVGAVDNTQENFWPSWYLQHVEVLWTTLN
                     QFNNTGLTMQDKRILFRTRECLPALFEGFNDNCVLIHGNFCLRSMLKDSRSDQLLAMV
                     GPGLMLWAPREYELFRLMDNSLAEDLLWSYLQRAPVAESFIWRRWLYVLWDEVAQLVN
                     TGRFSRRNFDLASKSLLPWLA"
     gene            complement(12830..13576)
                     /gene="kdsB"
                     /locus_tag="ACHOSC_RS05515"
                     /old_locus_tag="pgaptmp_001103"
     CDS             complement(12830..13576)
                     /gene="kdsB"
                     /locus_tag="ACHOSC_RS05515"
                     /old_locus_tag="pgaptmp_001103"
                     /EC_number="2.7.7.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008690 - 3-deoxy-manno-octulosonate
                     cytidylyltransferase activity [Evidence IEA];
                     GO_process: GO:0009244 - lipopolysaccharide core region
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-manno-octulosonate cytidylyltransferase"
                     /protein_id="HGU4547082.1"
                     /translation="MSFVVIIPARYASTRLPGKPLVDINGKPMIVHVLERARESGAER
                     IIVATDHEDVARAVEAAGGEVCMTRADHQSGTERLAEVVEKCAFSDDTVIVNVQGDEP
                     MIPATIIRQVADNLAQRQVGMATLAVPIHNAEEAFNPNAVKVVLDAEGYALYFSRATI
                     PWDRDRFAEGLETVGDNFLRHLGIYGYRAGFIRRYVNWQASPLEHIEMLEQLRVLWYG
                     EKIHVAVAQEVPGTGVDTPEDLERVRAEMR"
     gene            complement(13573..13755)
                     /gene="ycaR"
                     /locus_tag="ACHOSC_RS05520"
                     /old_locus_tag="pgaptmp_001104"
     CDS             complement(13573..13755)
                     /gene="ycaR"
                     /locus_tag="ACHOSC_RS05520"
                     /old_locus_tag="pgaptmp_001104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YcaR"
                     /protein_id="HGU4547083.1"
                     /translation="MDHRLLEIIACPVCNGKLWYNQEKRELICKLDNLAFPLRDGIPV
                     LLETEARVLTADESKS"
     gene            complement(13807..15039)
                     /gene="ycaQ"
                     /locus_tag="ACHOSC_RS05525"
                     /old_locus_tag="pgaptmp_001105"
     CDS             complement(13807..15039)
                     /gene="ycaQ"
                     /locus_tag="ACHOSC_RS05525"
                     /old_locus_tag="pgaptmp_001105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="crosslink repair DNA glycosylase YcaQ"
                     /protein_id="HGU4547084.1"
                     /translation="MSLPHLSLADARNLHLAAQGLLNKPRRQASLEDIPATISRMSLL
                     QIDTINIVARSPYLVLFSRLGNYPAQWLDESLARGELMEYWAHEACFMPRSDFRLIRH
                     RMLAPEKMGWKYKDAWMQEHEAEIAQLIQHIHDKGPVRSADFEHPRKGASGWWEWKPH
                     KRHLEGLFTAGKVMVIERRNFQRVYDLTHRVMPDWDDERDLVSQTEAEIIMLDNSARS
                     LGIFREQWLADYYRLKRPALAAWREARAEQQQIIAVHVEKLGNLWLHADLLPLLERAL
                     AGKLTATHSAVLSPFDPVVWDRKRAEQLFDFSYRLECYTPAPKRQYGYFVLPLLHRGQ
                     LVGRMDAKMHRQTGILEVISLWLQEGIKPTTMLQKGLRQAITDFASWQQATRVTLGRC
                     PQGLFTDCRTGWEIDPVA"
     gene            complement(15076..16062)
                     /gene="lpxK"
                     /locus_tag="ACHOSC_RS05530"
                     /old_locus_tag="pgaptmp_001106"
     CDS             complement(15076..16062)
                     /gene="lpxK"
                     /locus_tag="ACHOSC_RS05530"
                     /old_locus_tag="pgaptmp_001106"
                     /EC_number="2.7.1.130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009029 - tetraacyldisaccharide 4'-kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetraacyldisaccharide 4'-kinase"
                     /protein_id="HGU4547085.1"
                     /translation="MIEKIWSGESPLWRLLLPLSWLYGLVSGAIRLCYKLKLKRAWRA
                     PVPVVVVGNLTAGGNGKTPVVVWLVEQLQQRGIRVGVVSRGYGGKAESYPLLLSADTT
                     TAQAGDEPVLIYQRTDAPVAVSPVRSDAVKAILAQHPDVQIIVTDDGLQHYRLARDVE
                     IVVIDGVRRFGNGWWLPAGPMRERAGRLKSVDAVIVNGGVPRSGEIPMHLLPGQAVNL
                     RTGTRCDVAQLEHVVAMAGIGHPPRFFATLKMCGVQPEKCVPLADHQSLNHADVSALV
                     SAGQTLVMTEKDAVKCRAFAEENWWYLPVDAQLSGDEPAKLLTQLTSLASGN"
     gene            complement(16059..17807)
                     /gene="msbA"
                     /locus_tag="ACHOSC_RS05535"
                     /old_locus_tag="pgaptmp_001107"
     CDS             complement(16059..17807)
                     /gene="msbA"
                     /locus_tag="ACHOSC_RS05535"
                     /old_locus_tag="pgaptmp_001107"
                     /EC_number="7.5.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0034040 - ATPase-coupled lipid
                     transmembrane transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipid A ABC transporter ATP-binding
                     protein/permease MsbA"
                     /protein_id="HGU4547086.1"
                     /translation="MHNDKDLSTWQTFRRLWPTIAPFKAGLIVAGVALILNAASDTFM
                     LSLLKPLLDDGFGKTDRSVLVWMPLVVIGLMILRGITSYVSSYCISWVSGKVVMTMRR
                     RLFGHMMGMPVSFFDKQSTGTLLSRITYDSEQVASSSSGALITVVREGASIIGLFIMM
                     FYYSWQLSIILIVLAPIVSIAIRVVSKRFRNISKNMQNTMGQVTTSAEQMLKGHKEVL
                     IFGGQEVETKRFDKVSNRMRLQGMKMVSASSISDPIIQLIASLALAFVLYAASFPSVM
                     DSLTAGTITVVFSSMIALMRPLKSLTNVNAQFQRGMAACQTLFTILDSEQEKDEGKRV
                     IERATGDVEFRNVTFTYPGRDVPALRNINLKIPAGKTVALVGRSGSGKSTIASLITRF
                     YDIDEGEILMDGHDLREYTLASLRNQVALVSQNVHLFNDTVANNIAYARTEQYSREQI
                     EEAARMAYAMDFINKMDNGLDTVIGENGVLLSGGQRQRIAIARALLRDSPILILDEAT
                     SALDTESERAIQAALDELQKNRTSLVIAHRLSTIEKADEIVVVEDGVIVERGTHNDLL
                     EHRGVYAQLHKMQFGQ"
     gene            complement(17844..20108)
                     /locus_tag="ACHOSC_RS05540"
                     /old_locus_tag="pgaptmp_001108"
     CDS             complement(17844..20108)
                     /locus_tag="ACHOSC_RS05540"
                     /old_locus_tag="pgaptmp_001108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309023.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0030420 - establishment of competence for
                     transformation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ComEC family protein"
                     /protein_id="HGU4547087.1"
                     /translation="MKITTVGVCIICGIFPLLILPQLPGTLTLAFLTLFACVLAFIPV
                     KTVRYIALTLLFFVWGILSAKQILWAGETLTGATQDAIVEITATDGMTTHYGQITHLQ
                     GRRIFPAPGLVLYGEYLPQAVCAGQQWSMKLKVRAVHGQLNDGGFDSQRYAIAQHQPL
                     TGRFLQASVIEPNCSLRAQYLASLQTTLQPYMWNAVILGLGMGERLSVPKEIKNIMRD
                     TGTAHLMAISGLHIAFAALLAAGLIRGGQVYLPGRWIHWQMPLIGGICCAAFYAWLTG
                     MQPPALRTVVALATWGMLKLSGRQWSGWDVWICCLAAILLMDPVAILSQSLWLSAAAV
                     AALIFWYQWFPCPEWQLPPVLRAVVSLIHLQLGITLLLMPVQIVIFHGISLTSFIANL
                     LAIPLVTFITVPLILAAMVVHLSGPLILEQGLWFLADRSLALLFWGLKSLPEGWINIA
                     ERWQWLSFSPWFLLVVWRLNAWRTLPAMCVAVGLLMCWPLWQKPRPDEWQVYMLDVGQ
                     GLAMVIARNGKAILYDTGLAWPEGDSGQQLIIPWLHWHNLEPEGVILSHEHLDHRGGL
                     DSILHTWPMLWIRSPLNWEHHQPCVRGEAWQWQGLRFSVHWPLQASNDKGNNHSCVVK
                     VDDGTNSILLTGDIEVPAEQKMLSRYWQQVQTTLLQVPHHGSNTSSSLPLIQRVNGKV
                     ALASASRYNAWRLPSNKVKHRYQQQGYQWLDTPHQGQVTVNFSAQGWRISSLREQILP
                     RWYHQWFGVPVDNG"
     gene            complement(20315..20599)
                     /gene="ihfB"
                     /locus_tag="ACHOSC_RS05545"
                     /old_locus_tag="pgaptmp_001109"
     CDS             complement(20315..20599)
                     /gene="ihfB"
                     /locus_tag="ACHOSC_RS05545"
                     /old_locus_tag="pgaptmp_001109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006819309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005694 - chromosome [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0006417 - regulation of translation
                     [Evidence IEA];
                     GO_process: GO:0075713 - establishment of integrated
                     proviral latency [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="integration host factor subunit beta"
                     /protein_id="HGU4547088.1"
                     /translation="MTKSELIERLATQQSHIPAKTVEDAVKEMLEHMASTLAQGERIE
                     IRGFGSFSLHYRAPRTGRNPKTGDKVELEGKYVPHFKPGKELRDRANIYG"
     gene            complement(20759..22432)
                     /gene="rpsA"
                     /locus_tag="ACHOSC_RS05550"
                     /old_locus_tag="pgaptmp_001110"
     CDS             complement(20759..22432)
                     /gene="rpsA"
                     /locus_tag="ACHOSC_RS05550"
                     /old_locus_tag="pgaptmp_001110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S1"
                     /protein_id="HGU4547089.1"
                     /translation="MTESFAQLFEESLKEIETRPGSIVRGVVVAIDKDVVLVDAGLKS
                     ESAIPAEQFKNAQGELEIQVGDEVDVALDAVEDGFGETLLSREKAKRHEAWITLEKAY
                     EDAETVTGVINGKVKGGFTVELNGIRAFLPGSLVDVRPVRDTLHLEGKELEFKVIKLD
                     QKRNNVVVSRRAVIESENSAERDQLLENLQEGMEVKGIVKNLTDYGAFVDLGGVDGLL
                     HITDMAWKRVKHPSEIVNVGDEITVKVLKFDRERTRVSLGLKQLGEDPWVAIAKRYPE
                     GTKLTGRVTNLTDYGCFVEIEEGVEGLVHVSEMDWTNKNIHPSKVVNVGDVVEVMVLD
                     IDEERRRISLGLKQCKANPWQQFAETHNKGDRVEGKIKSITDFGIFIGLDGGIDGLVH
                     LSDISWNVAGEEAVREYKKGDEIAAVVLQVDAERERISLGVKQLAEDPFNNWVALNKK
                     GAIVTGKVTAVDAKGATVELADGVEGYLRASEASRDRVEDATLVLSVGDEVEAKFTGV
                     DRKNRAISLSVRAKDEADEKDAIATVNKQEDANFSNNAMAEAFKAAKGE"
     gene            complement(22543..23226)
                     /gene="cmk"
                     /locus_tag="ACHOSC_RS05555"
                     /old_locus_tag="pgaptmp_001111"
     CDS             complement(22543..23226)
                     /gene="cmk"
                     /locus_tag="ACHOSC_RS05555"
                     /old_locus_tag="pgaptmp_001111"
                     /EC_number="2.7.4.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000125007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004127 - (d)CMP kinase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(d)CMP kinase"
                     /protein_id="HGU4547090.1"
                     /translation="MTAIAPVITIDGPSGAGKGTLCKAMAEALQWHLLDSGAIYRVLA
                     LAALHHHVDVASEDALVPLASHLDVRFVSTNGNLEVILEGEDVSGEIRTQEVANAASQ
                     VAAFPRVREALLRRQRAFRELPGLIADGRDMGTVVFPDAPVKIFLDASSEERAHRRML
                     QLQEKGFSVNFERLLAEIKERDDRDRNRAVAPLVPAADALVLDSTTLSIEQVIEKALQ
                     YARQKLALA"
     gene            complement(23399..24163)
                     /gene="ycaL"
                     /locus_tag="ACHOSC_RS05560"
                     /old_locus_tag="pgaptmp_001112"
     CDS             complement(23399..24163)
                     /gene="ycaL"
                     /locus_tag="ACHOSC_RS05560"
                     /old_locus_tag="pgaptmp_001112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706828.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallopeptidase YcaL"
                     /protein_id="HGU4547091.1"
                     /translation="MKNTKLLLAIATSAALLTGCQNTHGIDTNMAISSGLNAYKAATL
                     SDADAKAIANQGCAEMDSGNQVASKSSKYGKRLAKIAKALGNNINGTPVNYKVYMTSD
                     VNAWAMANGCVRVYSGLMDMMNDNEIEGVLGHELGHVALGHSLAEMKASYAIVAARDA
                     ISATSGVASQLSRSQLGDIAEGAINAKYSRDKESEADDFSFDLLKKRGISTQGLVGSF
                     EKLASLDGGRTQSMFDSHPPSTERAQHIRDRIASGK"
     gene            complement(24332..25615)
                     /gene="aroA"
                     /locus_tag="ACHOSC_RS05565"
                     /old_locus_tag="pgaptmp_001113"
     CDS             complement(24332..25615)
                     /gene="aroA"
                     /locus_tag="ACHOSC_RS05565"
                     /old_locus_tag="pgaptmp_001113"
                     /EC_number="2.5.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003866 - 3-phosphoshikimate
                     1-carboxyvinyltransferase activity [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoshikimate 1-carboxyvinyltransferase"
                     /protein_id="HGU4547092.1"
                     /translation="MESLTLQPIARVDGTINLPGSKSVSNRALLLAALAHGKTVLTNL
                     LDSDDVRHMLNALTALGVSYTLSADRTRCEIIGNGGPLHAEGALELFLGNAGTAMRPL
                     AAALCLGSNDIVLTGEPRMKERPIGHLVDALRLGGAKITYLEQENYPPLRLQGGFTGG
                     NVDVDGSVSSQFLTALLMTAPLAPEDTVIRIKGDLVSKPYIDITLNLMKTFGVEIENQ
                     HYQQFVVKGGQSYQSPGTYLVEGDASSASYFLAAAAIKGGTVKVTGIGRNSMQGDIRF
                     ADVLEKMGATICWGDDYISCTRGELNAIDMDMNHIPDAAMTIATAALFAKGTTTLRNI
                     YNWRVKETDRLFAMATELRKVGAEVEEGHDYIRITPPEKLNFAEIATYNDHRMAMCFS
                     LVALSDTPVTILDPKCTAKTFPDYFEQLARISQAA"
     gene            complement(25686..26774)
                     /gene="serC"
                     /locus_tag="ACHOSC_RS05570"
                     /old_locus_tag="pgaptmp_001114"
     CDS             complement(25686..26774)
                     /gene="serC"
                     /locus_tag="ACHOSC_RS05570"
                     /old_locus_tag="pgaptmp_001114"
                     /EC_number="2.6.1.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0004648 -
                     O-phospho-L-serine:2-oxoglutarate aminotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006564 - L-serine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoserine/phosphohydroxythreonine
                     transaminase"
                     /protein_id="HGU4547093.1"
                     /translation="MAQIFNFSSGPAMLPAEVLKQAQQELRDWNGLGTSVMEVSHRGK
                     EFIQVAEEAEKDFRDLLNVPSNYKVLFCHGGGRGQFAAVPLNILGDKTTADYVDAGYW
                     AASAIKEAKKYCTPNVFDAKVTVDGLRAVKPMREWQLSDNAAYMHYCPNETIDGIAID
                     ETPDFGKDVVVAADFSSTILSRPIDVSRYGVIYAGAQKNIGPAGLTIVIVREDLLGKA
                     NIACPSILDYSILNDNGSMFNTPPTFAWYLSGLVFKWLKANGGVAEMDKINQQKAELL
                     YGVIDNSDFYRNDVAKANRSRMNVPFQLADSALDKLFLEESFAAGLHALKGHRVVGGM
                     RASIYNAMPLEGVKALTDFMVEFERRHG"
     gene            complement(26973..27665)
                     /locus_tag="ACHOSC_RS05575"
                     /old_locus_tag="pgaptmp_001115"
     CDS             complement(26973..27665)
                     /locus_tag="ACHOSC_RS05575"
                     /old_locus_tag="pgaptmp_001115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF421 domain-containing protein"
                     /protein_id="HGU4547094.1"
                     /translation="MKAFDLHRMAFDKVPFDFLGEVALRSLYTFVLVFLFLKMTGRRG
                     VRQMSLFEVLIILTLGSAAGDVAFYDDVPMVPVLIVFITLALLYRLVMWLMAHSEKLE
                     DLLEGKPVVIIEDGELAWSKLNNSNMTEFEFFMELRLRGVEQLGQVRLAILETNGQIS
                     VYFFEDDKVKPGLLILPSDCTQRYKVVPESADYACIRCSEIIHMKAGEKQLCPRCANP
                     EWTKASRAKRVT"
     gene            27795..29555
                     /gene="ycaO"
                     /locus_tag="ACHOSC_RS05580"
                     /old_locus_tag="pgaptmp_001116"
     CDS             27795..29555
                     /gene="ycaO"
                     /locus_tag="ACHOSC_RS05580"
                     /old_locus_tag="pgaptmp_001116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415425.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12 methylthiotransferase
                     accessory factor YcaO"
                     /protein_id="HGU4547095.1"
                     /translation="MTQTFIPGKDAALEDSIARFQQKLSDLGFQIEEASWLNPVPNVW
                     SVHIRDKECALCFTNGKGATKKAALASALGEYFERLSTNYFFADFWLGETIANGPFVH
                     YPNEKWFPLTENDDVPEGLLDDRLRAFYDPENELTGSMLIDLQSGNEDRGICGLPFTR
                     QSDNQTVYIPMNIIGNLYVSNGMSAGNTRNEARVQGLSEVFERYVKNRIIAESISLPE
                     IPADVLARYPAVVEAIETLEAEGFPIFAYDGSLGGQYPVICVVLFNPANGTCFASFGA
                     HPDFGVALERTVTELLQGRGLKDLDVFTPPTFDDEEVAEHTNLETHFIDSSGLISWDL
                     FKQDADYPFVDWNFSGTTEEEFATLMAIFNKEDKEVYIADYEHLGVYACRIIVPGMSD
                     IYPAEDLWLANNSMGSHLRETILSLPGSEWEKEDYLNLIEQLDEEGFDDFTRVRELLG
                     LATGSDNGWYTLRIGELKAMLALAGGDLEQALVWTEWTMEFNSSVFSPERANYYRCLQ
                     TLLLLAQEEDRQPLQYLNAFVRMYGADAVEAASAAMSGEAAFYGLQPVDSDLHAFAAH
                     QSLLKAYEKLQRAKAAFWAK"
BASE COUNT         7191 a         8057 c         7191 g         7177 t
ORIGIN      
        1 gtcccctgca atattttgaa tttgcacgat tttgtaggcc ggataaggcg ctcgtgccgc
       61 atccggcata aacaaagcgc actttgccag aaatccaggc ccgcattaaa agcgggcttt
      121 tttatgtgcc ttaccagacg ggcatttcgt cttgcaggaa gggattatgc aggcgttcat
      181 aaccaagtgt ggataatggt ccgtgacccg gaataaatgt cacgtcatcc cccagtggca
      241 gcaatttatc tttgattgaa gaaatcagtt gattatgatc gccacgcggg aagtcactgc
      301 gccctactcc gcctttgaaa ataacatcgc cagaaatcag cagctttgcc cgatcatcaa
      361 aaaacacgac atgacccggc gtatgcccag ggcaatgtaa cacctgtaaa gtcacattcc
      421 ctatgctgat ggtatcgcct tcgttcagcc aacgatctgg cgtcagcggc tggcactctt
      481 ccagaccaaa catacgactt tgcgcaggca agccttgcag ccagaactca tcttcttttt
      541 ccgggccaaa aaccggcacg ccgtaatgtt gcgccagttc cgccgctgcg ccaacgtggt
      601 ccagatgacc atgcgtcagc aggatctgca tcagtgtcag gccgctgtca tcaacttcct
      661 gtttgatttt ttccgcatcg ccgccaggat cgaccagtgc ggccagacgg gtttgttcac
      721 accagattaa tgaacagttc tgggagaatg cggtgaccgg aataatacga tagttcatac
      781 tgcccctgtt tcgttaagca attactacca gtgccgtgct ggcccggtat caatatgcac
      841 aaagttacta cgtggataat atcctacacc acctgcgcgc atagataacg cggctttgcg
      901 aatattgctt aacgcgatac cttcaatatg gaaatccatc gcctggcctt tagtgtgata
      961 gcttttcttc gctactccac ggctgcgggc gcgtagttca ttgttggtat caatagaacg
     1021 ataaccggaa atgagttgca ccggtttgcg cgtgcctaac aacccttgca ggcgatacaa
     1081 ctggtcgaat aatcctgggt cgatggactt tattttgttc gcgcggtaat cgcggaaaaa
     1141 atggttaagt tttgccaatt cttcctgaat atagcctctg ccatcgaaaa actccgcttt
     1201 gattgactct ccggtatgaa gattattgag tgtcaaaatg cgcgggcgtg gggtagagag
     1261 tgttgcaaac gcaggggtcg gcaggatggc ggcaccgagt gcaacgccac caagcgccag
     1321 caatttgcgg cgattagcgt cgaatttgtc catgataatc aagtctacag gtcaatgtta
     1381 tcgtttatat gcacttctgg cgcacaagag gcaccttaac cgcccgtaaa gcagacgtca
     1441 agatgactca accccagcag tgacgggggc tgcagagaat cgcccccaat catgctaatt
     1501 attacgacaa ctgatttccc cgaactactt catttacctg attaattgtt ccgctttcga
     1561 tacgatttgc gagctggatc gcgcaggcag atcataattg taaatatctg tacgatactg
     1621 ggtacgacca tctgcaccaa caaaggccgt caggtagtag agattcaccg gaatcgactg
     1681 ccgaatattg acgtaacgtg tatcaccctg cttcagcgca tcagaaatac gtttgtcatt
     1741 ccagcctgca tcctgcaaca gcatattcgc cagatcggaa gctttattca ctcgtacaca
     1801 gcctgagctc aatgcgcgtg tatcacgctt gaacagattg tggttcggcg tgtcatgcaa
     1861 ataaatggcc tctgaactcg gcatattgaa tttatagcgc cccagcgagt tccgtgggcc
     1921 tggagcctgc tggaagcgga acggtaaatt cgaggccgtg attgtagacc agtcaacctg
     1981 ccatgggtca atcgcttctc tgctgttcca gccgcgcatc accgtatagc catggctttc
     2041 gagatatccc ggatcgttgc gcacttttgg cagaatatct ttgcgtgcca gagttggagg
     2101 tacgttccac ggcgggttta ccactacgtt gttaagggca ctgctcatca tcggcgtttt
     2161 gcgatcgggg cgaccgacaa tgactcgcga atccagcacc tgattgccgt tctgatagta
     2221 gaccagcgaa taggccggaa tgttaaccat gatcccggta gaaagctctg ttggcagcaa
     2281 gcgcaatcgc tggatgttga gagccaacac gccagcacgc tgggcgggcg ttacgtttaa
     2341 ccagtcacgt gttgccgggc caatagcacc atctgccccc aatccttgcc atgcctgaaa
     2401 acgtttaacg gcttccacca gttcattatc gtaggcggca cgaacggcag gcgcaggttt
     2461 actacgagac gtcgtttgct tgtccatcgg cttagtttct gctgtttcaa cagtaacagc
     2521 ggatgggctg actaccgcgt cagttggcgt gtcatcgcca ggtagagtaa ttttcggccc
     2581 cccgtccaac atgcctgtgc gttgcaatat ttcgcgcaac gccggtacgt cgttactcca
     2641 ctgccctggg cgcaacgttg ctttgccggt cagttggggc cacggtttgg tgtcactgag
     2701 taaggccagt aacgattcat gcatcgccgc atattgcgga tgctgcggtg ccagtcctgc
     2761 aacaaacgta ggcaattgac ctttatccag cgccagctgc cattggttaa tcaccgagag
     2821 cggcggcgtt gcaagtgcat aaggtttact gctatatagc cagcgagtgc ctttgaccgg
     2881 aatatttgca atgaaatgga gatagcccat catcgcatca gagagcacta cgtcgcgtgc
     2941 catcccgtta acaccaggat cggtcagtaa ctctacccat ttattaaact gcggctggaa
     3001 accggcaatc gccacctctg ccagctgttg ctggaaggct ttaacagcat cacggttttc
     3061 ccacatgggt tgcatatcgc gtgcggcata caacagttga agctggttaa gataaaccgg
     3121 cttgtaacct gcgggtaatt gagattcgat ttgcgtgcgg gctttttctg ccgcaccttc
     3181 aggcaatggc tggatccccg ccattattgc cgtggcttgc gcctgcggca gtgcctcgcc
     3241 ctgttcactg acagccaccg ggctgtcgcc agggattact tcaggctcat cggcctgcgc
     3301 attgaacagt ggagcgaatg ttacggccag gcacaaactg attgccgaca gccgacgacc
     3361 acacatcata ttaagcaaca tcccttgccc cctgttttta tcatactctg aatagccagg
     3421 ctgttatttt acgacttgcc tgtacaacgc caatactcac gaaagtatga cgagcaagcc
     3481 tgttactcat caggcttatc ttcagtatat aaacgccgat acgtttttac ataacctaat
     3541 gtaaagaagt ttaaagaaaa gcgcgccgta atgtgccact tttttagtgc ggaaacggag
     3601 ttttcggaaa aagaaaaggc ggcattgctg ccgccttaat tttaactcgc ctgagaaggc
     3661 gcttcgtcac ttcctggaag cgtttcaggg agttgcggcg caaatcctcg caggccgacg
     3721 acgtgaacgt gttcggtatt ctggaagact ttacgcacca gtttataggt ggtgcctttc
     3781 tccgggctga tattttccgg cgctgcgatg atgagctgca tttgcaaacg ctcacacaat
     3841 tcaaacagcg tggcgataga acgagcatcc agtcgcgctg cttcatcgag gaacagcagg
     3901 cggcaaggag agatatcttt accgcgcagg cggcgagatt catcttccca gctttgtacc
     3961 accatcacca gaatcgacat accggtacca atcgcctcac cggtcgacaa tgcaccagac
     4021 tctgcgcgca accaaccatc ggaaccacgg ttaacctcaa cttccatttc cagatagttg
     4081 cggtaatcca gcagttcttc gccaatggtc tgcggcgtgc gctgtcccat atcaatctgc
     4141 ggattaagac gctgatacaa tttcgccaac gcttccgaga aggtcagacg gttgctgtta
     4201 aacagatcct gatgctgctc gtgctgttca gagagcacat ccagcagcat ggcgtgcgtt
     4261 tcacgcacgt tcacgttgag gcgcacgctg ttcacctgac caaacgatac gttctgcaac
     4321 ccctggttga gcatacggat acggttctgc tcgcgctgaa tggttttgcg aatgatgttc
     4381 gccacgctgc gggaactgat cgccagtttc tgttcacggg aggttaattc ttcggtcaga
     4441 cggctaagtt cgatctccat ctgttcgata gcttccaccg gatcatcggt acgaataata
     4501 tcctgacgaa tacgttcacg cagatgctga taaaccgcca cgaagaactg aattttacgc
     4561 tccggacgtt tcggatcttc cgacatgcgc agcacgtcgc gcagatgttc gttatccgcc
     4621 accgccagac gcagcgcacc caatgcctta tccgacatag agcgcaaatc atcagcggag
     4681 agataagcca gctcacgacg gtgtaagcgg cgctcaacgc cgttatcttt caccatgcgc
     4741 atcaccgcac accagcccgc tttggcggtc actacctgct cgcgcatctc aaagtaatcc
     4801 cgctccagct tgcgcagttt gcgggtcagg ttgtccatct ccgcttcgca gaaggtaagc
     4861 gctttttcca gttgattgcg gcgtgaacgg ttattgctca gttgcgcatg cagctcgtca
     4921 cggcgaatac gcgcccgctc ttctgccccg ctatcagcac gcacgccgat atcctgcaat
     4981 tcacgttgca gatcgttgag tagctctttt ttggtgtcgt aagaactttt cagcgaagcc
     5041 agcacctggt tgtactgact caactgcgct gcgtgaccac gcaacgcttc acgagcgcga
     5101 gtacgctccg cttccgcctg ttccagacgt tcacgcagct tttcgttgag atcgctatta
     5161 ccgctcagca tttctgccga gtcagaatag ctaaagtgcg cacgacgctg caccacttcc
     5221 gtcagggcaa acgcctgctg acgggcatcg cgctgcatct gctgagagta cgcgtaatct
     5281 tcttttagct gttcgaactg ttccgggtcg ctctgcaata ccgaaacgat cggttccagt
     5341 ttcgccagtt gattgccaaa ctgctgaaca aaacgcgcgg cttcctgggc ttcatccaga
     5401 cgttcgcgga tttcatcgac gcgatccgcc aggctgtcat cagccaacag gttgagacgc
     5461 ggcagaatgc ggttcagcgc cgtaacgcct tctttcgcct gctcaaactg aatacgctgc
     5521 tgctggttat cattttcatg attactcaac gcccgctcca gttcgacgcg acggctgttc
     5581 agttgacgga tttctgcttc cgggtcagac tcaaacgcaa ccgccagatg actgccgata
     5641 aagcggctga acgcctgatg cagacgctga gttttctgta catcaaagga gagcgtggcg
     5701 aagcgttcgg aaagcacttc acgctcggca tggaggcttt caatacggct ttcacgcgca
     5761 gcacgaccaa acagcggcac ttccgggaaa cgtgaataac gccactgacg atcggcgatt
     5821 ttcaccacta ccgctttttc cagctcatca acgctgaaca cgctgtcatc gaatgactgc
     5881 ggatctcctt cgatcagata gagatcttcc gggcaattgg tcaagccttc caggtgttca
     5941 gttatctgtg acagatctgg caccacgatg gcgtggcgtg acgggccata cagcgctgag
     6001 aagtacggcg catcttccag gctaacgtcg tcataaattt ctgacagcag cacaccacca
     6061 aaacgctccg ccagcgcgtt cagacgctga tcttcagagc cgccaggctg gcttaaacgt
     6121 tcgatctctt catcgacggc gtttttgcgc gcgcccactt catcgcgttc aacaatcgcc
     6181 tctcgctcac gctccagcaa ctgttgcaga tattcggtga cgtcctggct ggaggtaaac
     6241 tcttcgccgc actgttcgct caactggttg agactgtttt gcgctgccag ccaaaccggc
     6301 gcacgctgca tcaaactctg aatgcgagac tgcagctgtt cctgttcctg gcgcagtgcc
     6361 atgcgctctt cacgggcgtt agacacgcta tcggaaagag aggcaatgcg cgcttccagc
     6421 tcctgatgca gagcttccag ctcgtcaatg tcaaaattct tgccctgacg tttgcagaaa
     6481 tctgccagca gacgctcagc ttcttgctgt tcgcgcagac gctgttccag ttcgcttaag
     6541 cgcatccgca acggctgaac ctgctctgcc aggtgacgct gatcgacccc ttcgcgcaat
     6601 agttcgcgag cgacatccca cgcctcgtta cgcgccagcg ggccgttgat tgccaccacc
     6661 agctgataag cctgctcaaa ctggctgtgc gcggtttgcg ccatgctcat tttctgctca
     6721 agagagagca ttttttcagt cgcttccagc tctttcgcct ggaaggtttc cagccattcg
     6781 gcggcgctgt cggcggttaa gtccggcaga tggcacagtt ctttggcgcg attaagcgca
     6841 gcaatcgcct ggttatactg gatcgcgcgc gtttgctgga cgtccagcgc ctgctggtag
     6901 ttagcaagct ggcttttcag ctcgtccact tccagttcgg cagcttccgc gcgagcctcg
     6961 ttctcttcct ggcgttcgat ggcttctgcc accacttcgt tttgctcttc cagacggatc
     7021 tgcagctcat cgagatccgc ttcgtagcgt tcgatcttct cctgctgacg cagtgcggtt
     7081 tgcaccaggt tcaggtgatc gctggccgcc tgataatccg cttccagatc gccttcggca
     7141 ccgttgtgct ctgccagctc acgcgccata tcgacgtgtt tgtactgctc agccgccagt
     7201 tgctgacgcg aagtatgtaa ctcgcgacga aactccaggg ctttgtcgag atggacacgg
     7261 cgctcgttgg cgtgacgcat gtagtccgcc gccacgtagt tggtagcttc gctgatcaga
     7321 tgcttaaaca ggtcacggtc cgactgggtg acacgaatcg cttccagcgt catgcggttt
     7381 tcacgcagcg ccgcttccat gtcctggaac gctttacgca cgccgctgtt ttccggcaac
     7441 aggtagtcgc gcagagaacg ggtaatggca ctggagatcc cgccatacag cgaagcttcg
     7501 atcagacgat agaatttgct acggtcagat gcggagcgca gacgacgcgc gatgatgccc
     7561 agatcgaaca tcagcgagtg gtaatcggta atggagttga actgtttaaa ctgcacgcct
     7621 tccatcgcct cgagcttgtc tttcagctcg ttaagcggca gcacgcgcgc ctggcgttcg
     7681 ttcagggttt cggtcaccag ctgtgtcggc tgcaccgaca tcggcagtcc ctgaatggca
     7741 aatggtttga tatccacttt acgatcgcgt ccggcaacct gttgcagacg cacaccgacc
     7801 accacgcgct ggtggcgcga gttaatggtg tcgagcatcg aataacagac acccgctttc
     7861 agcttaccgt gcagaccttt atcgcgcgaa ccgctggtgg ccccggcttc cgtagtgtta
     7921 cggaaatgca gcagggtcag gtcggggatc agcgccgtaa cgaacgccgc catggtggtg
     7981 gatttacccg ccccgttacc gccagaaagc gtcgtgacca gctcgtcaag gtcaaaagtt
     8041 cgggcaaaaa agccgttcca gttaatcagc gtcagtgagc gaaatttacc gcgttcaatc
     8101 attattcttc ctctccgcta tctggctgat tttcttcggt ttcatcgttg agttgcagat
     8161 gattttcaat cggcattgct tcgccatcac gaatcaggcg acgctgtgct tcacggggat
     8221 cgtcgccagc acgcacatcg gctccgaagc ggaacaccga ttcggtaatg cgaaacttgc
     8281 tgctgtcgtg gcccataaac cacaccatgc ctaaacgacg cagacggttg agcgaagaac
     8341 gtactttctc ctgcaacttc tgacggtcaa cgtctgaacc ggttgaacgg ttgttcacca
     8401 gtttcagcag ttttgcttca tcggccaggg tgagcagttc gtcgtacagt tcctgctggg
     8461 tgaaaatccc ctcattcgcc agccgttccg ggctgagata gagataacag aggattttcc
     8521 cgaccatcat atccagttcc gacaagacgg aacgagggat cagcgtggtg gaacgtgggc
     8581 gtaaatagaa gaacccttct ggtgcgcgaa taagctcaac gttataacgc gcgtaaaact
     8641 cttccagata ttcctgaaaa tccatcagga atgcatgatt atccagttcg tcgaggccaa
     8701 tatggcggcc tgaacgtaag gcgctgtcca gcgccggaaa taacggattc gccagcgcct
     8761 gcgccagctt aaccggcatc acttgttcaa tatttgtcga tgacatgcgc ctgtaccttg
     8821 gctccgtaat cattaatcgg ctgccatttc gctggcagtc cggtgaaatc tgcttgcgct
     8881 acgccaagac gtaccgcctg atcaataaca atacgcgcaa cgtcaaagtg acgtgcacgc
     8941 ggatactgtg acagatattc gcgtaccacc agaccaagat ccagcggcac ttgtctggtt
     9001 ttgtacacgg caagttgttc ttcgatgatc gccgccagct gttcgcggat ctcgttaaac
     9061 tcttcgtatt ccagatcctc aggaagttcc ccagtcactt cttcatcgcg cagtgccatc
     9121 tcttcgtcac gcatatccag cagacgatcg gcattggcat aagttagcgc ccacggctca
     9181 tcaaaatagg tttgtaccga ctgacgtaac cgctgagcaa agacgcggtt tttatccata
     9241 tcgatcgcgg tacgaataaa tttgtgtacg tggcggtcgt agccaatcca caagtcgatg
     9301 gattgctggc cccaactgat aatacgatcg agtttgctct gcagatcgaa caccagacga
     9361 tcgacgaaat gcagatcgtc atgggtcatc gtcgcatcct gaatgcgcaa cagattagcc
     9421 tgcaatttgt cgcctgccgc ttccagcgta tcctgcaatt cacgcagcgt cccggaagtt
     9481 tccgaaagca acaattcaca gctggaaatc gccgcccgcc agtctttgtt cagcaactgg
     9541 gcgatatcgt ccttcacctg ctgctgctgt tcgtccatca gacgttgcgt caggtcgata
     9601 ctgtcgaaaa tttctgctac cgaatatttc agtggcgcat agacattacg gtgccagtga
     9661 aattcatcac cgccctcttc ggcggcatct gctgcgcgtt tgagctcacc cgccacaatc
     9721 gacaactgca tagaaagacg cagcgtagaa aactcgcgct gacggatgta gtagtcagta
     9781 atgccgatgc cgagcggcgt cagacggtaa attgcgttcc cttccgcctg ctcgctggta
     9841 aagcggttca gcagacgttg acgcaccatg tcgttgatcg cgttattggc gcgcacgccg
     9901 atggtttcgc tggtttgctc aaacgcatca ctcacatggc ggaatgcatc caccagctcg
     9961 ccttcactca tctcaccatc cagacgctcg ccgttcagcg tggcaaccgc cagcagaaaa
    10021 gagagtcggt ctaccggcag cgagatggag aagtcatttt ttctggccca ggcaaccagt
    10081 tcggggactg tctgggaaaa ttcactcata ctttatcctt gctctgcggt ttgcgcgcgg
    10141 tgacatgaat ataacgcccc agggtaatat acggttcctg acggcaataa cgcgtttcta
    10201 attcaagtaa tgcttcatag cagtcgcgct gctggtgctt ctcgcgcaga taatcatgaa
    10261 acacgcgaac gcctgtctta cccataattt gccaaccagc ttcttccagc cacagataaa
    10321 cctgcgccgg gtcgcgtgga taatctggcg aaagcgtccg ttttttcttt ttcggcattc
    10381 ccgcctgcac gtaatcaaaa ttcccggcga ccatgttatg catcaacaaa ccatgcgcat
    10441 tgtagaacat taacgacaac acgccgcctg gacgcaacac tgaccacagg gtctgcaata
    10501 cactgcgggg atcggccacc cactcgagca ccgcatggaa caatatcaga tcaacgggcg
    10561 tttccaaatg cgaagcaaca tcctgagcgg cgcaatgtat aaattgcatg ttgtcgctca
    10621 caccttttgc ttctgccgcc tgttttgcgc ggtcgatcat ctgcgcagaa agatcgcata
    10681 aaatgacctg atgcccacgc tcggccattt tgattgcggt ctgcccttct ccaccgccag
    10741 catccagcac acgcagtttt tgcgggccca tttccgccag cacgcgatcg agatcctgcc
    10801 acagaatagc ctgtcgaagc tgccctttgg tggtgccgta aatgttacgg gaaaactttt
    10861 ccgcaatatc atcaaaattg cgatcctgca ttgctttctc caccggttgg ctaagacgtt
    10921 ttctgataaa gacgctattt tgtcacagcc acggcgagaa tgaacccgtc tggtgtaata
    10981 tcgctggcga tcccttgcta tatggttaaa aaaggaacca gaaaggatgc tttttacact
    11041 gaaaaaagtg attggcaata tgttgctgcc acttccgctg atgttgttaa ttattggcgc
    11101 tggcctggcg ctgctgtggt ttagccgttt tcaaaaaacc gggaagattt ttattagcat
    11161 tgggtggctg gcactcttgt tattgagttt gcaaccggta gccgaccgcc tgctgcgtcc
    11221 catcgaaagc acctatccga catggaataa ttcgcaaaaa gtggactata tcgtggtgct
    11281 cggagatggt tatacctgga acccgcagtg ggcaccgagc tctaatttaa tcaataacag
    11341 tctgccacgc ctgaatgaag gtattcgctt atggcgcgaa aatccgggat caaaactgat
    11401 cttcacggga ggcgtagcaa aaaccaatac ggtgagtaca gcggaagtag gtgccagagt
    11461 tgcgcaatcg ctgggcgtgc cgcgcgagca aattatcacc ctggatttgc caaaagatac
    11521 cgaagaagaa gctgcggcag tgaaacaggc gattggtgac gcccctttcc tgctggtgac
    11581 ctccgcatca catctgccgc gggcgatgat cttttttcag caggaagggt taaatccgct
    11641 accagccccg gcgaatcagt tggccatcga ctcgccgctt aacccgtggg aacgggcaat
    11701 cccttctccg gtctggctga tgcatagcga tcgtgttggc tatgaaacgc ttggccgcat
    11761 ctggcagtgg ctgaaaggtc cgtcaggcga gccacggcaa gagtgatttt gatgccagat
    11821 cgaagttgcg ccgactaaat cgtccggtat taaccagttg cgcaacttca tcccataaca
    11881 catacaacca acgccgccag atgaacgact ccgccactgg cgcgcgttgc aggtaactcc
    11941 agagcaaatc ttccgccaga gaattatcca ttagtcggaa cagttcgtat tctcgcggtg
    12001 cccaaagcat tagtcccggc ccgaccatcg ccagtaactg atcgctgcgc gaatctttca
    12061 gcatgctgcg taaacagaag ttaccgtgaa tcaacacaca attgtcgtta aagccttcaa
    12121 ataatgccgg gagacattcg cgagtgcgaa acaggatccg cttatcctgc atcgtcagac
    12181 cggtgttatt gaactgattg agcgtggtcc atagcacttc aacatgttgc aggtaccatg
    12241 agggccagaa attttcctga gtattgtcga ccgcgccgac gcaaccgcga ctgtcctgac
    12301 ggtgccaggc cagtaaggct tcaacgattt ggtctttgag ctgttcccag cgttctggtg
    12361 ttcgggctgg tgcctccacc gaaacaccac gcatccgctc cagtagcagg acatcagggc
    12421 cggggtgttc ttcatgcgtc atcacaccat aaatagtcgg catacggaca gtcccacttc
    12481 ttgccagcat ggtggttttc catgccagtt gtcgggcttt tccgggcgta ctaaagcttc
    12541 ttgccattaa cggcattggg tttccctggc tgtcatacaa agcccacaac gccgtatccg
    12601 ctttttcatt gacgcactca atacgactga gtttttcgcc cagtaaatgg cttaattcgg
    12661 cacgcagctg ttccattcag attaccctca taaaagctac tgctttcata atgagcgcca
    12721 atataaaaga tgtcacctga atgagatcaa ttgttgaaaa gaaaaattga ggaaaatcgt
    12781 aacggtacga cactcctccc aaaattggct gaagtgtcgt gaagtgaaat tagcgcattt
    12841 cagcgcgaac gcgctcaaga tcttcagggg tatccacacc tgtgccagga acttcctgag
    12901 caacagcaac atggattttt tcgccgtacc acagaacacg aagctgctct aacatttcga
    12961 tgtgttctaa cggacttgcc tgccagttga cgtaacgacg gataaagcct gcacggtagc
    13021 cataaatacc aagatgacgc aggaagttat cgccaacggt ttcaaggcct tctgcaaaac
    13081 gatcacgatc ccaaggaatg gtggcgcgtg agaagtacag tgcataccct tcagcgtcga
    13141 gaaccacttt caccgcattc gggttaaacg cttcttccgc attgtggatt ggcaccgcca
    13201 gagtcgccat acccacctga cgctgagcga ggttatcagc aacctgacga atgattgtcg
    13261 cagggatcat cggttcatca ccctgcacat taacgatcac cgtgtcgtcg ctgaatgcgc
    13321 atttttcgac aacttccgcc agacgttctg ttcctgactg atgatcggcg cgcgtcatac
    13381 atacttcacc gccagcggct tcaacggcgc gggcaacatc ctcatgatcg gttgccacga
    13441 tgatgcgctc ggcacctgat tcacgcgcgc gttcaagaac atgaacaatc atgggtttgc
    13501 cgttaatatc aaccaatggt ttaccgggca gacgcgtgga cgcgtagcgc gcgggaataa
    13561 tgaccacaaa actcatgatt tactctcatc agcagtcagc acgcgggctt ccgtttccag
    13621 caacactgga atgccatcgc ggagcgggaa agccaggtta tccagtttgc aaataagctc
    13681 tcgtttttcc tggttatacc aaagttttcc gttgcaaacc gggcaggcaa tgatttcaag
    13741 caaacgatga tccatagttc ctccagatgg atcgggttat gaatgcataa atcttatcat
    13801 aatcatttat gcgacggggt ctatttccca gccagtgcgg caatcagtaa agagaccttg
    13861 cgggcagcgt cctaatgtca cccgcgttgc ctgctgccag ctcgcgaaat cagtaatcgc
    13921 ctgacgtaac cctttttgca gcattgtcgt tggtttaatg ccttcctgta accacagaga
    13981 gataacttca aggatgcctg tctggcgatg cattttggca tccattcgcc caactaattg
    14041 cccacgatgt aataatggca gaacaaaata gccatactgg cgtttcggcg ctggggtata
    14101 gcactccagc cggtagctaa aatcaaaaag ctgctctgcg cgtttgcgat cccagacaac
    14161 aggatcaaaa ggcgaaagta ccgcgctgtg cgttgcagtg agctttccgg ccagcgcacg
    14221 ctcgagtagc ggcagcaaat cagcatgcag ccaaagattg cccaattttt caacatgcac
    14281 agcaatgatt tgctgctgtt cagccctcgc ttcgcgccac gccgccagtg ccggacgttt
    14341 cagccgatag taatctgcca gccactgttc gcggaatatt cccaggctac gcgcactgtt
    14401 atccagcatg atgatttctg cttctgtttg cgaaacgaga tcgcgctcat catcccagtc
    14461 aggcatgaca cggtgggtta aatcataaac gcgctggaag ttgcgccgtt caatcaccat
    14521 cacctttccg gcagtaaata aaccttccag atgccgttta tgcggcttcc attcccacca
    14581 gccgcttgca cctttacgag gatgctcaaa atcggctgaa cgtaccggcc ccttatcatg
    14641 aatatgctga attaactgtg caatttccgc ctcatgttcc tgcatccagg cgtctttgta
    14701 tttccagccc attttttcag gtgccagcat gcggtggcga ataagacgaa agtcgctacg
    14761 cggcataaag caggcttcat gcgcccagta ttccattaat tcgccacgcg ccagagactc
    14821 atccagccac tgggcaggat aatttcccag acgactgaaa agcaccagat atggactacg
    14881 ggcaacaata ttgatggtat cgatttgcag caaggacatg cgggagatcg ttgccggaat
    14941 atcctccaac gacgcttgac ggcggggttt gtttaacagg ccttgtgcgg caaggtgaag
    15001 attacgcgca tcagcaaggg agaggtgcgg cagcgacatt catgactcca tcaatcgaac
    15061 gctgccgcgg cgtaactagt tgccagaagc cagcgaggtt agttgcgtaa gcagtttcgc
    15121 tggttcatca cctgaaagct gtgcgtctac aggcaaatac caccaatttt cttctgcaaa
    15181 ggcccggcat ttcaccgcat ctttttcagt cattaccagc gtttgcccgg cgcttaccaa
    15241 cgcactgaca tccgcatggt tcaaagactg atgatcggcc agcggtacac atttttccgg
    15301 ttgtacgcca cacatcttca gcgtggcaaa aaagcgcggc ggatgcccaa tccccgccat
    15361 cgccactaca tgttcaagct gagcaacgtc acaacgcgta ccggtacgta aattcaccgc
    15421 ctgacccggc agcagatgca tggggatttc accgctgcga gggacaccgc cgttgacgat
    15481 taccgcatca accgacttta agcgccccgc tcgctcacgc attggccccg ccggcaacca
    15541 ccagccattg ccaaagcgac gcacaccatc aataacgaca atttccacat cacgcgccag
    15601 acggtaatgc tgtaaaccgt cgtcggttac gatgatctgc acatcagggt gttgcgccag
    15661 aatggctttt accgcatcag aacgaacggg agaaaccgca acaggcgcat cagtgcgttg
    15721 ataaatcaac acaggttcat cacccgcctg tgctgttgtg gtatctgccg acaataacag
    15781 cggataagat tcagccttac caccatatcc ccgcgatacg acccccacgc gaataccgcg
    15841 ctgttgcaac tgttccacca gccagacaac gaccggggtt tttccgttgc cgcctgcggt
    15901 gagattacca accacgacaa ccggtacggg ggcacgccag gcgcgcttca gttttagttt
    15961 atagcaaaga cggatcgcgc cacttaccag gccatacaac caggagagtg gcagcaatag
    16021 ccgccacaaa ggggattcac cagaccagat tttttcgatc attggccaaa ctgcattttg
    16081 tgaagttgcg cgtaaacgcc gcggtgctca agcaaatcgt tatgcgtacc gcgttccaca
    16141 atgacaccat cctcgacgac cacgatttcg tctgcctttt caatggtaga caagcggtgg
    16201 gcaatcacca gagaggtacg gtttttctgc aactcatcca gtgccgcctg aatcgcacgt
    16261 tcggattcgg tatccagagc cgaggtagct tcatccagaa tcagaatcgg gctatcacgc
    16321 agcaaggctc gagcgatagc aatacgctga cgctgaccgc cagagagcag cacgccgttt
    16381 tcaccaatca ctgtatcgag accgttatcc atcttattga tgaagtccat ggcgtaggcc
    16441 atacgcgccg cttcttcaat ttgctcacgg ctgtactgtt cagtccgtgc gtaagcaatg
    16501 ttgttagcaa ccgtatcgtt aaacagatgg acattctgcg acaccagagc aacctggtta
    16561 cgtaacgacg ccagggtata ctcgcgcaga tcgtgaccat ccatcaggat ttcgccttca
    16621 tcaatatcgt aaaaacgagt gatcaggctg gcgatggttg atttacctga accagagcgt
    16681 ccaaccagag caaccgtctt ccctgccgga attttcaggt tgatgttacg caatgcaggt
    16741 acgtcacgtc ccggataagt aaaggtgaca ttgcggaatt ccacgtcgcc agtcgcacgc
    16801 tcgatcacgc gcttaccttc gtctttctcc tgctcactgt ccagaatggt aaacagcgtc
    16861 tgacaagccg ccataccgcg ctggaactgg gcgttaacgt tggtcagtga tttcagcgga
    16921 cgcatcagtg caatcattga agagaaaaca acggtaatcg taccggcagt caggctatcc
    16981 atgacacttg ggaagctcgc cgcatacaga acaaacgcca gcgccaaaga ggcgatcagc
    17041 tgaatgatcg gatcagagat ggaagaggct gaaaccattt tcatcccctg aagacgcatt
    17101 cggttgctga ctttatcaaa gcgtttcgtt tccacttcct gaccaccgaa aatcaatact
    17161 tctttgtggc ccttcagcat ttgttcggcg ctggtggtca cctgccccat ggtgttctgc
    17221 atgtttttac tgatgttgcg aaaacgcttc gatactacgc gaatcgcaat cgaaacaatc
    17281 ggtgccagca caatcaaaat gatcgacagt tgccaactgt aatagaacat catgatgaac
    17341 aggccgatga ttgacgcacc ttcacgcaca accgttatca gtgcgccgga agaagaagaa
    17401 gcaacctgtt cggaatcgta ggtgatacgt gacaacagcg tccccgttga ctgtttgtca
    17461 aaaaatgaaa ccggcattcc catcatgtga ccaaacaggc ggcgacgcat ggtcattacc
    17521 acctttcctg atacccagga gatacagtag ctggagacat agctggtgat accacgtaaa
    17581 atcatcagcc cgatcaccac cagcggcatc cacaccagca cggagcgatc tgttttacca
    17641 aagccatcat caagaagtgg cttaaggagc gataacatga aggtatcgct ggctgcgttg
    17701 aggattaacg ctacgcccgc cacgatcaga cccgctttaa aaggcgcaat ggttggccac
    17761 agtcggcgga acgtctgcca cgtagagaga tctttgtcgt tatgcattca aaaaaccagc
    17821 atttgttgaa atagccgcat attctacccg ttatccactg gcacgccaaa ccactgatga
    17881 taccaacgag gtaaaatttg ctctctgagg ctgctaatcc gccagccttg cgctgaaaaa
    17941 ttgaccgtca cttgaccctg atgtggagta tcaagccatt gatatccttg ctgttgatag
    18001 cgatgcttaa ctttattaga gggcaatcgc catgcgttat agcgcgatgc cgatgcgagt
    18061 gccacttttc cattcactcg ctgaattaat ggcaacgatg atgaggtatt actgccatgg
    18121 tgaggtacct gaagcaatgt tgtctgcact tgctgccagt aacggcttag catcttttgt
    18181 tcagcgggga cttcaatatc accggttaga agaatgctat tcgtcccgtc atcaacctta
    18241 accacacagg aatggttatt tcctttatcg ttgctagctt gtaaaggcca gtgcacgctg
    18301 aaacgcaatc cttgccattg ccacgcttcg ccacgcacac agggctgatg atgttcccag
    18361 tttaacggac ttctgatcca taacatcggc catgtgtgca atattgagtc cagccctccc
    18421 cggtgatcca gatgttcatg actcagaata acgccttccg gttcaagatt atgccagtgg
    18481 agccagggga taatcagttg ttgcccacta tccccttcgg gccaggccag tcctgtgtca
    18541 tagagaatcg ctttgccgtt tctggctatc accattgcca gcccttgccc gacatcaagc
    18601 atgtacacct gccactcgtc aggtcgaggt ttttgccaca gcggccagca catcagcaag
    18661 cctacagcca cacacattgc tggcaacgtt cgccaggcgt ttaatcgcca taccaccagt
    18721 aagaaccatg gggaaaatga tagccattgc caacgttcag caatgttgat ccacccttct
    18781 ggcaaactct ttaaccccca gaaaagtaaa gccaaagacc ggtcggcaag aaaccataac
    18841 ccttgctcca ggattaacgg cccgcttaaa tgcacaacca tcgcggcgag gatcaacgga
    18901 accgtgataa atgtcaccaa gggaattgct aatagatttg caataaacga ggtcagacta
    18961 atgccatgaa atatgacgat ttgcacaggc ataagcagga gtgtgattcc cagttgcaaa
    19021 tgaatgaggg aaacaactgc acgcaatacc ggcggcagtt gccactcagg acagggaaac
    19081 cactggtacc aaaaaatcag tgccgcgacc gcagcggcag agagccataa actttgcgag
    19141 agaatggcaa caggatccat cagcaaaatt gccgccagac aacatatcca tacatcccag
    19201 ccactccact gtcgcccact taacttaagc attccccacg tagcaagcgc caccacggta
    19261 cgcaatgcag gaggttgcat tcccgtcaac caggcataaa aagcagcaca gcagattcca
    19321 ccaattaatg gcatttgcca gtggatccag cgcccaggca gataaacttg cccaccgcga
    19381 atgagtccgg cagccagcaa cgccgcaaaa gcgatgtgca atcccgatat cgccattaaa
    19441 tgcgccgttc cagtatcacg catgatattt ttgatttctt tggggacgga taaccgttcc
    19501 cccataccta aaccaagaat aaccgcattc cacatatagg gttgcagcgt tgtttgtagt
    19561 gacgccagat actgtgcacg caggctacaa ttcggttcaa tgacacttgc ctgcagaaag
    19621 cggccggtga gcggctgatg ctgggcaatg gcataacgct ggctatcaaa gccgccatca
    19681 ttaagttggc catgaactgc acgaactttg agtttcattg accattgttg tccagcacaa
    19741 accgcttgcg gaagatattc gccatacagc acaaggcctg gcgcagggaa tatacgtcga
    19801 ccttgtagat gagtaatttg accgtaatga gtggtcatgc cgtcagttgc agtgatctca
    19861 acaattgcat cctgcgtcgc gccagttaag gtttctcctg cccacaaaat ttgctttgct
    19921 gataatatgc cccaaacgaa aaacagcaac gtcagcgcga tataacggac ggttttaaca
    19981 gggataaatg ccagtacgca ggcgaagaga gtcagaaacg caagggttaa tgtcccaggc
    20041 aattggggca aaatcagcaa cggaaaaatt ccgcaaatta tgcatacgcc gactgtcgtt
    20101 attttcatgc acccctccct gtttatggaa agtgtgcaac tttgttgagc ggttgtctgg
    20161 tgggaatttt gcgttccccg aggcacattc agaagaattt aacgaaaggg tgaaaactgc
    20221 tcaaaactct gcgaggcagc ttccagttcc tgattagaga aatgaaaaaa gcacccgaca
    20281 ggtgcttttc tcgcgttcaa gtttgagtaa aaacttaacc gtaaatattg gcgcgatcgc
    20341 gcagttcttt accaggttta aagtgaggaa cgtattttcc ttccagttct actttatcgc
    20401 cagtcttcgg attacgtccg gtacgtggtg cgcggtagtg caaagagaaa ctgccgaaac
    20461 cgcggatttc aatacgctcg ccctgcgcaa gagtcgaggc catatgctcc agcatctctt
    20521 ttactgcatc ttcaaccgtc ttggcgggaa tgtgcgattg ctgggtggca agtctttcta
    20581 tcaattctga cttggtcatg attcctccgg ttccttaaag gcaaattaag cggctgctgt
    20641 tgctgcattg attaagggcg gccgtagccg cccttagtgc ttgattacag gacgaaacct
    20701 gcaatctgtc aagtaaactc aacaaacttc ggaataaaaa tcccgaagag tcagagaatt
    20761 actcgccttt agctgctttg aaagcttcag ccattgcgtt gttggagaag tttgcatctt
    20821 cctgtttgtt aacagttgcg attgcatctt tctcgtcagc ttcgtctttc gcacgaacag
    20881 acaggctgat tgcgcggttt ttacgatcaa cgccggtgaa tttagcttca acttcgtcgc
    20941 caacgctcag aaccagggta gcgtcttcaa cgcggtcacg ggatgcttca gaagcacgca
    21001 ggtaaccttc aacgccgtca gccagttcta cggttgcgcc tttagcgtca actgcagtta
    21061 ctttaccggt tacgatagcg cctttcttgt tcagagcaac ccagttgttg aacggatctt
    21121 ctgcgagctg tttaacgccc agggagatac gttcacgttc tgcgtcaacc tgcagaacaa
    21181 ctgcagcgat ttcgtcgcct tttttgtatt cacgaactgc ttcttcgcct gcaacgttcc
    21241 aggagatgtc agacaggtga accaggccgt cgatgccgcc gtccaggccg atgaagatac
    21301 cgaagtcagt gatagacttg attttacctt caacacggtc gcccttgttg tgggtttccg
    21361 cgaactgctg ccacgggtta gctttgcact gtttcagacc cagggagata cgacgacgtt
    21421 cttcgtcgat atccagaacc ataacttcca ctacatcgcc aacgttaaca actttggacg
    21481 ggtggatgtt tttgttggtc caatccattt cggaaacgtg taccaggcct tcaacgcctt
    21541 cttcgatttc aacgaagcag ccgtagtcgg tcaggttggt cacgcgacca gtcagtttgg
    21601 taccttccgg ataacgttta gcgatagcta cccacggatc ttcgcccaac tgtttcaggc
    21661 ccagggatac acgggtacgt tcgcggtcga acttcagcac tttaacagtg atttcgtcgc
    21721 ccacgttgac gatttcgctc ggatgcttaa cgcgtttcca ggccatgtca gtgatgtgca
    21781 gcaggccgtc aacgccgccc agatcaacga atgcaccgta gtcagtgagg ttcttaacga
    21841 tacctttaac ttccatgcct tcctgcaggt tttccagcag ctgatcgcgc tctgcgctgt
    21901 tttcggattc gataacggca cgacgagaaa caacaacgtt gttgcgcttc tgatccagct
    21961 tgattacttt aaattcaagc tctttgcctt ccaggtgcag agtgtcacgc accggacgaa
    22021 cgtctaccag agaacctggc aggaacgcac gaataccgtt cagctcaaca gtgaagccgc
    22081 ccttaacttt gccgttgata acaccggtaa cagtttcagc atcttcgtaa gctttttcca
    22141 gcgtgatcca ggcttcgtga cgtttagctt tctcacggga cagcagagtt tcaccgaagc
    22201 cgtcttctac tgcgtccaga gcaacgtcaa cttcatcacc tacctggatt tccagctcgc
    22261 cctgggcgtt tttgaactgc tcagccggga tggcggactc agatttcaga ccagcgtcaa
    22321 ccagtactac gtctttgtcg atagcaacaa caacgccacg aacgatagaa cccgggcggg
    22381 tttcgatttc ttttaaggac tcttcaaaga gttgagcaaa agattcagtc atgtttaatc
    22441 ttcaggttta tatttaacgt ccacctggct ccatgccgga tggggttgtt taacataccc
    22501 gctgtcattc cattgcaacg ggggtactgc aaattcggtc gcttatgcga gagccaattt
    22561 ctggcgcgcg tattgtagcg ctttttcaat cacttgctca atgcttaagg tggtggaatc
    22621 caacactaaa gcatcggctg ccggaaccag tggcgctacc gctcggttac gatcgcggtc
    22681 gtcgcgttct ttgatctcgg ccaaaaggcg ctcaaagtta acactaaagc ccttctcctg
    22741 caactgtagc atgcggcgat gcgcacgttc ttccgaggag gcgtcaagga aaattttcac
    22801 tggcgcatca gggaatacca ccgttcccat gtcgcggcca tcggcaatca ggccgggtaa
    22861 ttcgcgaaac gcgcgttggc gacgcaataa tgcttcacga acgcgtggga atgccgcgac
    22921 ttgtgaagct gcattcgcca cttcctgagt acgaatttcg ccgctgacat cttccccttc
    22981 gaggatcact tccagattgc cattggttga cacaaaacgt acatccagat gggatgccag
    23041 cggtaccagc gcatcttctg acgcaacatc aacatggtga tgtaatgccg ccaatgccag
    23101 tacgcgataa attgcacccg aatccagcag atgccattgc aatgcttccg ccatagcctt
    23161 acacaaggtg cctttccctg caccgcttgg gccatcaatg gtaataaccg gggcaattgc
    23221 cgtcatcttt atctccttaa aacaggcgta ccgttaacat aaccgcgcgc attatacgcg
    23281 ccgacgccac aaatcgttaa cgttgtgtgc aaaaaccagt tttgtctgta ttcagatggg
    23341 gatttattgc gcaattatag cgggctggca gaagaccagc ccgaaagatg acaatgattt
    23401 acttaccaga ggcgatacga tcacggatgt gttgcgcacg ctctgttgat ggtgggtgag
    23461 agtcaaacat ggactgggtg cgaccaccat ccagactagc cagtttttca aagctgccaa
    23521 ccagcccctg ggtgctgatg ccacgtttct tcaacagatc aaaggagaaa tcatctgctt
    23581 cggactcttt atcacgggag tatttagcat taatagcgcc ttctgcgata tcacccaatt
    23641 gtgagcggga aagctgggaa gccacaccgc tggtagctga aatggcatcg cgtgcggcaa
    23701 cgatcgcata agaagctttc atttcagcca gcgagtgacc caacgcgacg tggcccagtt
    23761 catggcccag aacaccttca atttcgttgt cattcatcat gtccatcagg ccactgtaga
    23821 cacggacaca gccgttcgcc atcgcccatg cgttgacgtc gctggtcata taaaccttat
    23881 agttgaccgg cgtgccatta atattgttac ccaatgcttt ggcgattttt gccagacgtt
    23941 taccgtactt gctggattta cttgcgactt gattgccgct gtccatttcg gcacagccct
    24001 gattggcaat cgcttttgca tcggcatcgc ttaatgttgc tgctttatag gcatttaaac
    24061 cggagctgat agccatattg gtatcaatac cgtgggtatt ttgacaccct gtcagtaatg
    24121 ctgcagaggt cgcaatcgcc agcagtaatt tagtattctt catgtgaatc atccgttgat
    24181 taatcatata taaatattat gtatcaacaa cttacattga caagcgacgc taccattaat
    24241 caaaatgact cacaaggtcc gaaaaaaaac gccgacaatg tgccgacgtt ttgataaaga
    24301 aagatttggc tatttattgc ccgttgttca ttcaggctgc ctggctaatc cgcgccaact
    24361 gctcgaaata atccggaaat gttttggccg tacatttggg atcaagaatc gtcactggtg
    24421 tatctgacaa cgccaccagc gagaaacaca tcgccatccg gtgatcattg tacgtcgcga
    24481 tctcggcaaa gttcagtttt tccggtggag tgatacgaat gtaatcgtgc ccctcttcta
    24541 cttccgcacc gactttacgc agttctgttg ccatcgcaaa caggcgatcg gtttctttaa
    24601 cacgccagtt atagatattg cgcagcgtgg tggtgccttt tgcaaataac gccgccgtgg
    24661 caatggtcat cgccgcatcg ggaatatggt tcatatccat atcaatagcg ttcagttcac
    24721 cacgcgtgca ggaaatataa tcatcgcccc agcaaatggt cgcgcccatt ttttccagca
    24781 catcagcaaa gcgaatatca ccctgcatac tgttacgtcc aataccggtc acttttacag
    24841 tgccgccttt gattgctgct gctgccagaa agtaagaagc cgaagatgca tcgccttcga
    24901 ccaaataagt acccggagac tgataagact gcccgccttt tacgacaaat tgttgatagt
    24961 gctgattttc aatttcaaca ccaaacgtct tcatcagatt gagtgtgatg tcgatataag
    25021 gtttagaaac cagatcgcct ttaatacgaa tcaccgtatc ttccggcgca agaggcgcag
    25081 tcattaacag tgcggtgagg aattggctgg aaacggagcc atcaacgtca acgttgccgc
    25141 cagtaaagcc gccctgtaaa cgcaacggcg gataattttc ttgttccagg taagtgatct
    25201 tcgccccgcc caggcgcagc gcatccacca gatgaccaat cgggcgttct ttcatacgcg
    25261 gctcaccggt cagcacaata tcattgctac ccagacaaag agctgccgcc agcggacgca
    25321 ttgccgttcc ggcgttaccg aggaacaact ccagggcacc ttctgcgtgt aatggaccgc
    25381 cgttaccgat aatttcgcaa cgcgtacgat cggctgaaag cgtatagctt acccctaacg
    25441 ctgttaatgc attcagcata tggcgcacgt catcgctatc cagcagattg gttaatactg
    25501 ttttgccgtg tgctaatgcc gccagcaata aagcgcggtt agaaacgctc ttggaaccgg
    25561 gcagattaat agtgccatcg acacgagcga tgggttgtaa cgtcagggat tccatgaact
    25621 caactctcta caacagaaat aaaaacccca caggctggct gtggggatta agcaaaattt
    25681 cggcattaac cgtgacggcg ttcgaactca accatgaagt ctgtcagcgc tttaacgcct
    25741 tccagcggca tggcgttata aatagaagcg cgcattccgc cgaccacacg gtgacctttc
    25801 agtgcatgaa ggccagcagc aaaagactct tcaaggaaca atttgtcaag cgcactgtcc
    25861 gccaactgga acggcacgtt catccgcgaa cggttagctt tcgccacgtc attgcggtag
    25921 aaatcgctgt tatcaatcac cccatatagc agttctgctt tttgctgatt gattttatcc
    25981 atttcagcta caccgccgtt cgctttcagc catttaaaga ccagaccaga tagataccag
    26041 gcaaatgtcg gcggcgtgtt aaacatggag ccgttatcgt tgaggatgga ataatccaga
    26101 atcgacggac acgcgatatt cgctttgccc agcaaatctt cacgaacgat gacgattgtc
    26161 aggccagccg ggccgatatt tttctgcgcg ccagcgtaaa tcacgccata acggctgacg
    26221 tcaatcggac gggaaagaat ggttgaagag aagtcggcgg cgaccaccac atctttgccg
    26281 aagtctggcg tttcgtcgat ggcgataccg tcgatggttt cattcgggca gtaatgcata
    26341 taagcagcat tatcagagag ttgccattca cgcattggct taaccgcgcg cagaccatca
    26401 acagtcactt tggcgtcaaa gacattaggc gtgcagtatt ttttcgcttc tttaatggca
    26461 cttgccgccc agtaaccggc atcaacataa tctgcggtgg ttttatcacc gagaatattc
    26521 agcggtaccg cagcaaactg accgcgacca ccgccatggc agaataatac cttgtagttg
    26581 gaggggacat taagaagatc gcgaaaatcc ttctcggctt cctctgcaac ctgaatgaac
    26641 tctttgccac ggtgactcac ttccatcacc gacgtaccaa gaccgttcca gtcgcgcagt
    26701 tcctgttgag cctgtttaag cacctctgcc ggtagcattg ccggaccaga actaaaattg
    26761 aagatttgag ccatttcccc tcaccacgtt gcgttgctat taacccgctg tcaatcgatt
    26821 gcgaccacag gtaatacatt attcctgtgg ctatcggttt tatcattcag tgacacacat
    26881 cgcaatgagt aaaaccgttg agggcaaaaa tgtggcttgc atcacacaaa agaatctctc
    26941 ggattattag caaaaagccg atgtttttac tgtcaggtca cccgttttgc ccgacttgcc
    27001 ttcgtccatt ctggatttgc acagcgcgga cataattgtt tttcccccgc tttcatatga
    27061 atgatttcac tacaacgaat acaggcatag tccgccgact ccggcaccac tttgtaacgc
    27121 tgcgtacaat cactgggtaa aataagtaaa cccggtttca ccttgtcatc ttcaaagaaa
    27181 tagacactga tttgcccgtt ggtttcgaga atcgccagac gtacctgccc cagctgctcc
    27241 acgccacgca atcgtagctc cataaagaac tcaaattccg tcatgttgga gttattgagt
    27301 ttcgaccagg ccagctcgcc atcttcaata ataacaaccg gcttaccttc cagtagatct
    27361 tccagttttt cactgtgcgc catcaaccac attaccaggc ggtataacaa cgccagagtg
    27421 ataaagacga taagtaccgg caccatcggc acatcatcat aaaacgccac atctcccgcc
    27481 gctgaaccca gcgtgagaat gattaacact tcaaacagcg acatctgtcg cacaccgcgt
    27541 cttccggtca ttttgaggaa caaaaaaacc agtacaaagg tataaagact acgtagtgca
    27601 acttcgccaa ggaaatcaaa aggcacttta tcaaatgcca tacggtggag atcgaacgcc
    27661 ttcattttta tgctcttatc gtggtttatc cgctaaaagc atagtcaaca gttcatcggc
    27721 atggcggcgg tgatagcgcc tttgagataa aacccgtatt attgcgcgct ttccgtacga
    27781 ctaaagtgat tttcatgacg caaacattta tccccggcaa agatgccgct ctggaagatt
    27841 ccatcgctcg cttccagcaa aaactttcag acctcggctt tcagattgaa gaggcctcct
    27901 ggctgaatcc tgtgcctaac gtctggtctg tacatattcg cgacaaagag tgcgcactgt
    27961 gttttaccaa cggaaaaggc gcaaccaaaa aagcggcgct ggcttctgca cttggtgaat
    28021 atttcgagcg tctctcaacc aactactttt ttgctgattt ctggctgggc gaaaccatcg
    28081 ccaacggtcc gttcgtgcat tatcccaacg aaaaatggtt cccactgacc gaaaatgacg
    28141 atgtgccaga aggactactc gatgaccgtc tgcgcgcgtt ttatgatccg gagaatgaac
    28201 tgaccggcag catgctgatt gacctacaat ccggtaacga agatcgtggt atttgcggcc
    28261 tgccgtttac gcgtcagtcc gacaatcaga ccgtttatat tccgatgaat atcattggta
    28321 acctgtacgt ctccaacggt atgtccgcag gtaatacccg caacgaagca cgcgttcagg
    28381 gattgtctga agttttcgaa cgctacgtga aaaaccgcat tattgctgaa agcatcagcc
    28441 tgccagagat cccggcagac gtgctggcgc gttacccagc agtagttgaa gccatcgaaa
    28501 cgctggaagc ggagggtttc ccaatcttcg cttatgacgg ttcgcttggc ggccagtatc
    28561 cggtgatttg cgtggtactg ttcaatcctg ctaacggtac ctgctttgcc tctttcggtg
    28621 cgcatcctga ttttggcgta gcactggaac gtaccgtgac cgagctgctg caaggtcgtg
    28681 gcctgaaaga tttggatgtg tttactccgc caaccttcga tgatgaagaa gtcgctgaac
    28741 ataccaacct cgaaacgcac tttatcgatt ccagcggttt aatctcctgg gacctgttca
    28801 agcaggatgc cgattatccg tttgtggact ggaatttctc cggcaccacg gaagaagagt
    28861 ttgctacgct gatggctatc ttcaacaaag aagataaaga agtttatatt gccgattacg
    28921 agcatctggg cgtttatgct tgccgtatta tcgtgcctgg catgtccgat atttatccgg
    28981 ctgaagatct gtggctcgcg aataacagta tgggcagcca tttacgtgaa acgattcttt
    29041 cgctaccagg cagcgagtgg gaaaaagaag attacctgaa cctcatcgag caactggatg
    29101 aagaaggttt tgatgacttt acccgcgtgc gtgagctgtt gggtctggcg accgggtcgg
    29161 ataacggttg gtacaccctg cgtatcggtg aattaaaagc catgctggcg ctggctggtg
    29221 gcgatctgga acaggctctg gtctggaccg aatggacgat ggagtttaac tcatcagtat
    29281 ttagtccgga acgcgccaac tattatcgct gcctgcaaac gttgttatta ctggcacagg
    29341 aagaagatcg ccagccgctg caatatctga atgcgtttgt tcgcatgtac ggcgcagatg
    29401 ccgtagaagc cgccagtgcg gcaatgagcg gcgaagcggc gttttacggc ctgcaaccgg
    29461 tagatagcga tctgcacgcg tttgctgcac atcagtcgtt gttgaaggcc tacgaaaagc
    29521 tgcagcgcgc caaagcagca ttctgggcaa aataaaatca aatagcctac gcaatgtagg
    29581 cttaatgatt agtctgagtt atattacggg gcgttt
//
LOCUS       DAZKHF020000031        28426 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2201S31241
            SAMN43267800-rid25106413.denovo.031, whole genome shotgun sequence.
ACCESSION   DAZKHF020000031 DAZKHF020000000
VERSION     DAZKHF020000031.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267800
            Sequence Read Archive: SRR30307263
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 28426)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 28426)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 28426)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350581.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350581.2
            Genome Coverage       :: 151x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:21:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,652
            CDSs (total)                      :: 5,557
            Genes (coding)                    :: 5,320
            CDSs (with protein)               :: 5,320
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 237
            CDSs (without protein)            :: 237
            Pseudo Genes (ambiguous residues) :: 0 of 237
            Pseudo Genes (frameshifted)       :: 102 of 237
            Pseudo Genes (incomplete)         :: 130 of 237
            Pseudo Genes (internal stop)      :: 52 of 237
            Pseudo Genes (multiple problems)  :: 41 of 237
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28426
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267800-rid25106413.denovo.031"
                     /serotype="O 26:H11"
                     /isolate="2201S31241"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-01"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            119..661
                     /gene="zapC"
                     /locus_tag="ACHOSC_RS05585"
                     /old_locus_tag="pgaptmp_001117"
     CDS             119..661
                     /gene="zapC"
                     /locus_tag="ACHOSC_RS05585"
                     /old_locus_tag="pgaptmp_001117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415466.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapC"
                     /protein_id="HGU4547096.1"
                     /translation="MRIKPDDNWRWYYDEEHDRMMLDLANGMLFRSRFARKMLTPDAF
                     SPAGFCVDDAALYFSFEEKCRDFNLSKEQKAELVLNALVAIRYLKPQMPKSWHFVSHG
                     EMWVPMPGDAACVWLSDTHEQVNLLVVESGENAALCLLAQPCVVIAGRAMQLGDAIKI
                     MNDRLKPQVNVDSFSLEQAV"
     gene            complement(658..1767)
                     /gene="ycbX"
                     /locus_tag="ACHOSC_RS05590"
                     /old_locus_tag="pgaptmp_001118"
     CDS             complement(658..1767)
                     /gene="ycbX"
                     /locus_tag="ACHOSC_RS05590"
                     /old_locus_tag="pgaptmp_001118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-N-hydroxylaminopurine resistance protein YcbX"
                     /protein_id="HGU4547097.1"
                     /translation="MATLTRLFIHPVKSMRGIGLTHTLADVSGLAFDRIFMITEPDGT
                     FITARQFPQMVRFTPSPVHDGLHLTAPDGSSAYVRFADFATQDAPTEVWGTHFTARIA
                     PGAINKWLSGFFSREVQLRWVGPQMTRRVKRHNTVPLSFADGYPYLLANEASLRDLQQ
                     RCPASVKMEQFRPNLVVSGASAWEEDRWKVIRIGDVVFDVVKPCSRCIFTTVSPEKGQ
                     KHPAGEPLKTLQSFRTAQDNGDVDFGQNLIARNSGVIRVGDEVEILATAPAKIYGAAA
                     ADDTANITQQPDANVDIDWQGQAFRGNNQQVLLEQLENQGIRIPYSCRAGICGSCRVQ
                     LLEGEVTPLKKSAMGDDGTILCCSCVPKTALKLAR"
     gene            2011..4119
                     /gene="rlmKL"
                     /locus_tag="ACHOSC_RS05595"
                     /old_locus_tag="pgaptmp_001119"
     CDS             2011..4119
                     /gene="rlmKL"
                     /locus_tag="ACHOSC_RS05595"
                     /old_locus_tag="pgaptmp_001119"
                     /EC_number="2.1.1.173"
                     /EC_number="2.1.1.264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0008990 - rRNA
                     (guanine-N2-)-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 23S rRNA
                     (guanine(2069)-N(7))-methyltransferase RlmK/23S rRNA
                     (guanine(2445)-N(2))-methyltransferase RlmL"
                     /protein_id="HGU4547098.1"
                     /translation="MNSLFASTARGLEELLKTELENLGAVECQVVQGGVHFKGDTRLV
                     YQSLMWSRLASRIMLPLGECKVYSDLDLYLGVQAINWTEMFNPGATFAVHFSGLNDTI
                     RNSQYGAMKVKDAIVDAFTRKNLPRPNVDRDAPDIRVNVWLHKETASIALDLSGDGLH
                     LRGYRDRAGIAPIKETLAAAIVMRSGWQPGTPLLDPMCGSGTLLIEAAMLATDRAPGL
                     HRGRWGFSGWAQHDEAIWQEVKAEAQTRARKGLAEYSSHFYGSDSDARVIQRARTNAR
                     LAGIGELITFEVKDVAQLTNPLPKGPYGTVLSNPPYGERLDSEPALIALHSLLGRIMK
                     NQFGGWNLSLFSASPDLLSCLQLRADKQYKAKNGPLDCVQKNYHVAESTPDSKPAMVA
                     EDYANRLRKNLKKFEKWARQEGIECYRLYDADLPEYNVAVDRYADWVVVQEYAPPKTI
                     DAHKARQRLFDIIAATISVLGIAPNKLVLKTRERQKGKNQYQKLGEKGEFLEVTEYNA
                     HLLVNLTDYLDTGLFLDHRIARRMLGQMSKGKDFLNLFSYTGSATVHAGLGGARSTTT
                     VDMSRTYLEWAERNLRLNGLTGRAHRLIQADCLAWLREANEQFDLIFIDPPTFSNSKR
                     MEDAFDVQRDHLALMKDLKRLLRAGGTIMFSNNKRGFRMDLGGLAKLGLKAQEITQKT
                     LSQDFARNRQIHNCWLITAA"
     gene            4131..6038
                     /gene="uup"
                     /locus_tag="ACHOSC_RS05600"
                     /old_locus_tag="pgaptmp_001120"
     CDS             4131..6038
                     /gene="uup"
                     /locus_tag="ACHOSC_RS05600"
                     /old_locus_tag="pgaptmp_001120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HGU4547099.1"
                     /translation="MSLISMHGAWLSFSDAPLLDNAELHIEDNERVCLVGRNGAGKST
                     LMKILNREQGLDDGRIIYEQDLIVARLQQDPPRNVEGSVYDFVAEGIEEQAEYLKRYH
                     DISRLVMNDPSEKNLNELAKVQEQLDHHNLWQLENRINEVLAQLGLDPNVALSSLSGG
                     WLRKAALGRALVSNPRVLLLDEPTNHLDIETIDWLEGFLKTFNGTIIFISHDRSFIRN
                     MATRIVDLDRGKLVTYPGNYDQYLQEKEEALRVEELQNAEFDRKLAQEEVWIRQGIKA
                     RRTRNEGRVRALKAMRRERGERREVMGTAKMQVEEASRSGKIVFEMEDVCYQVDGKQL
                     VKDFSAQVLRGDKIALIGPNGCGKTTLLKLMLGQLQADSGRIHVGTKLEVAYFDQHRA
                     ELDPDKTVMDNLAEGKQEVMVNGKPRHVLGYLQDFLFHPKRAMTPVRALSGGERNRLL
                     LARLFLKPSNLLILDEPTNDLDVETLELLEELIDSYQGTVLLVSHDRQFVDNTVTECW
                     IFEGGGKIGRYVGGYHDARGQQEQYVALKQPAVKKTEEAAAAKAETVKRSSSKLSYKL
                     QRELEQLPQLLEDLEAKLEALQTQVADASFFSQPHEQTQKVLADMAAAEQELEQAFER
                     WEYLEALKNGG"
     gene            6168..7421
                     /gene="pqiA"
                     /locus_tag="ACHOSC_RS05605"
                     /old_locus_tag="pgaptmp_001121"
     CDS             6168..7421
                     /gene="pqiA"
                     /locus_tag="ACHOSC_RS05605"
                     /old_locus_tag="pgaptmp_001121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane integrity-associated transporter
                     subunit PqiA"
                     /protein_id="HGU4547100.1"
                     /translation="MCEHHHAAKHILCSQCDMLVALPRLEHGQKAACPRCGTTLTVAW
                     DAPRQRPTAYALAALFMLLLSNLFPFVNMNVAGVTSEITLLEIPGVLFSEDYASLGTF
                     FLLFVQLVPAFCLITILLLVNRAELPVRLKEQLARVLFQLKTWGMAEIFLAGVLVSFV
                     KLMAYGSIGVGSSFLPWCLFCVLQLRAFQCVDRRWLWDDIAPMPELRQPLKPGVTGIR
                     QGLRSCSCCTAILPADEPVCPRCSTKGYVRRRNSLQWTLALLVTSIMLYLPANILPIM
                     VTDLLGSKMPSTILAGVILLWSEGSYPVAAVIFLASIMVPTLKMIAIAWLCWDAKGHG
                     KRDSERMHLIYEVVEFVGRWSMIDVFVIAVLSALVRMGGLMSIYPAMGALMFALVVIM
                     TMFSAMTFDPRLSWDRQPESEHEES"
     gene            7426..9066
                     /gene="pqiB"
                     /locus_tag="ACHOSC_RS05610"
                     /old_locus_tag="pgaptmp_001122"
     CDS             7426..9066
                     /gene="pqiB"
                     /locus_tag="ACHOSC_RS05610"
                     /old_locus_tag="pgaptmp_001122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="intermembrane transport protein PqiB"
                     /protein_id="HGU4547101.1"
                     /translation="MESNNGEAKIQKVKNWSPVWIFPIVTALIGAWVLFYHYSHQGPE
                     VTLITANAEGIEGGKTTIKSRSVDVGVVESATLADDLTHVEIKARLNSGMEKLLHKDT
                     VFWVVKPQIGREGISGLGTLLSGVYIELQPGAKGSKMDKYDLLDSPPLAPPDAKGIRV
                     ILDSKKAGQLSPGDPVLFRGYRVGSVETSTFDTQKRNISYQLFINAPYDRLVTNNVRF
                     WKDSGIAVDLTSAGMRVEMGSLTTLLSGGVSFDVPEGLDLGQPVAPKTAFVLYDDQKS
                     IQDSLYTDHIDYLMFFKDSVRGLQPGAPVEFRGIRLGTVSKVPFFAPNMRQTFNDDYR
                     IPVLIRIEPERLKMQLGENADVVEHLGELLKRGLRGSLKTGNLVTGALYVDLDFYPNT
                     PAITGIREFNGYQIIPTVSGGLAQIQQRLMEALDKINKLPLNPMIEQATSTLSESQRT
                     MKNLQTTLDSMNKILASQSMQQLPTDMQSTLRELNRSMQGFQPGSAAYNKMVADMQRL
                     DQVLRELQPVLKTLNEKSNALVFEAKDKKDPEPKRAKQ"
     gene            9063..9626
                     /gene="pqiC"
                     /locus_tag="ACHOSC_RS05615"
                     /old_locus_tag="pgaptmp_001123"
     CDS             9063..9626
                     /gene="pqiC"
                     /locus_tag="ACHOSC_RS05615"
                     /old_locus_tag="pgaptmp_001123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane integrity-associated transporter
                     subunit PqiC"
                     /protein_id="HGU4547102.1"
                     /translation="MKKWLVTIAALWLAGCSSGEINKNYYQLPVVQSGTQSTASQGNR
                     LLWVEQVTVPDYLAGNGVVYQTSDVKYVIANNNLWASPLDQQLRNTLVANLSTQLPGW
                     VVASQPLGSAQDTLNVTVTEFNGRYDGKVIVSGEWLLNHQGQLIKRPFRLEGVQTQDG
                     YDEMVKVLASAWSQEAASIAQEIKRLP"
     gene            9882..10049
                     /gene="rmf"
                     /locus_tag="ACHOSC_RS05620"
                     /old_locus_tag="pgaptmp_001124"
     CDS             9882..10049
                     /gene="rmf"
                     /locus_tag="ACHOSC_RS05620"
                     /old_locus_tag="pgaptmp_001124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0141014 - ribosome hibernation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome modulation factor"
                     /protein_id="HGU4547103.1"
                     /translation="MKRQKRDRLERAHQRGYQAGIAGRSKEMCPYQTLNQRSQWLGGW
                     REAMADRVVMA"
     gene            complement(10119..10637)
                     /gene="fabA"
                     /locus_tag="ACHOSC_RS05625"
                     /old_locus_tag="pgaptmp_001125"
     CDS             complement(10119..10637)
                     /gene="fabA"
                     /locus_tag="ACHOSC_RS05625"
                     /old_locus_tag="pgaptmp_001125"
                     /EC_number="4.2.1.59"
                     /EC_number="5.3.3.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014915875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008693 -
                     (3R)-3-hydroxydecanoyl-[acyl-carrier-protein] dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 3-hydroxydecanoyl-ACP
                     dehydratase/trans-2-decenoyl-ACP isomerase"
                     /protein_id="HGU4547104.1"
                     /translation="MVDKRESYTKEDLLASGRGELFGAKGPQLPAPNMLMMDRVVKMT
                     ETGGNFDKGYVEAELDINPDLWFFGCHFIGDPVMPGCLGLDAMWQLVGFYLGWLGGEG
                     KGRALGVGEVKFTGQVLPTAKKVTYRIHFKRIVNRRLIMGLADGEVLVDGRLIYTASD
                     LKVGLFQDTSAF"
     gene            complement(10706..12466)
                     /gene="ycbZ"
                     /locus_tag="ACHOSC_RS05630"
                     /old_locus_tag="pgaptmp_001126"
     CDS             complement(10706..12466)
                     /gene="ycbZ"
                     /locus_tag="ACHOSC_RS05630"
                     /old_locus_tag="pgaptmp_001126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HGU4547105.1"
                     /translation="MTITKLAWRDLVPDTDSYQEIFAQPHLIDENDPLFSDTQPRLQF
                     ALEQLLHTRASSSFMLAKAPEESEYLNLIANAARTLQSDAGQLVGGHYEVSGHSIRLR
                     HAVSADDNFATLTQVVAADWVEAEQLFGCLRQFNGDITLQPGLVHQANGGILIISLRT
                     LLAQPLLWMRLKNIVNRERFDWVAFDESRPLPVSVPSMPLKLKVILVGERESLSDFQE
                     MEPELSEQAIYSEFEDTLQIVDAESVTQWCRWVTFTARHNHLPAPGADAWPVLIREAA
                     RYTGEQETLPLSPQWILRQCKEVASLCDGDTFSGEQLNLMLQQREWREGFLAERMQDE
                     ILQEQILIETEGERIGQINALSVIEFPGHPRAFGEPSRISCVVHIGDGEFTDIERKAE
                     LGGNIHAKGMMIMQAFLMSELQLEQQIPFSASLTFEQSYSEVDGDSASMAELCALISA
                     LADVPVNQSIAITGSVDQFGRAQPVGGLNEKIEGFFAICQQRELTGKQGVIIPTANVR
                     HLSLHSELVKAVEEGKFTIWAVDDVTDALPLLLNLVWDGEGQTTLMQTIQERIAQASQ
                     QEGRHRFPWPLRWLNWFIPN"
     gene            12652..13104
                     /gene="matP"
                     /locus_tag="ACHOSC_RS05635"
                     /old_locus_tag="pgaptmp_001127"
     CDS             12652..13104
                     /gene="matP"
                     /locus_tag="ACHOSC_RS05635"
                     /old_locus_tag="pgaptmp_001127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="macrodomain Ter protein MatP"
                     /protein_id="HGU4547106.1"
                     /translation="MKYQQLENLESGWKWKYLVKKHREGELITRYIEASAAQEAVDVL
                     LSLENEPVLVNGWIDKHMNPELVNRMKQTIRARRKRHFNAEHQHTRKKSIDLEFIVWQ
                     RLAGLAQRRGKTLSETIVQLIEDAENKEKYANKMSSLKQDLQALLGKE"
     gene            complement(13179..14219)
                     /gene="ompA"
                     /locus_tag="ACHOSC_RS05640"
                     /old_locus_tag="pgaptmp_001128"
     CDS             complement(13179..14219)
                     /gene="ompA"
                     /locus_tag="ACHOSC_RS05640"
                     /old_locus_tag="pgaptmp_001128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porin OmpA"
                     /protein_id="HGU4547107.1"
                     /translation="MKKTAIAIAVALAGFATVAQAAPKDNTWYTGAKLGWSQYHDTGF
                     INNNGPTHENQLGAGAFGGYQVNPYVGFEMGYDWLGRMPYKGSVENGAYKAQGVQLTA
                     KLGYPITDDLDIYTRLGGMVWRADTKSNVYGKNHDTGVSPVFAGGVEYAITPEIATRL
                     EYQWTNNIGDAHTIGTRPDNGMLSLGVSYRFGQGEAAPVVAPAPAPAPEVQTKHFTLK
                     SDVLFNFNKATLKPEGQAALDQLYSQLSNLDPKDGSVVVLGYTDRIGSDAYNQGLSER
                     RAQSVVDYLISKGIPADKISARGMGESNPVTGNTCDNVKQRAALIDCLAPDRRVEIEV
                     KGIKDVVTQPQA"
     gene            complement(14576..15085)
                     /gene="sulA"
                     /locus_tag="ACHOSC_RS05645"
                     /old_locus_tag="pgaptmp_001129"
     CDS             complement(14576..15085)
                     /gene="sulA"
                     /locus_tag="ACHOSC_RS05645"
                     /old_locus_tag="pgaptmp_001129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0009432 - SOS response [Evidence IEA];
                     GO_process: GO:0051782 - negative regulation of cell
                     division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SOS-induced cell division inhibitor SulA"
                     /protein_id="HGU4547108.1"
                     /translation="MYTSGYAHRSSSFSSAASKIARVSTENTTAGLISEVVYREDQPM
                     MTQLLLLPLLQQLGQQSRWQLWLTPQQKLSREWVQASGLPLTKVMQISQLSPCHTVES
                     MVRALRTGNYSVVIGWLADDLTEEEHAELVDAANEGNAMGFIMRPVSASSHATRQLSG
                     LKIHSNLYH"
     gene            15304..15933
                     /gene="sxy"
                     /locus_tag="ACHOSC_RS05650"
                     /old_locus_tag="pgaptmp_001130"
     CDS             15304..15933
                     /gene="sxy"
                     /locus_tag="ACHOSC_RS05650"
                     /old_locus_tag="pgaptmp_001130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CRP-S regulon transcriptional coactivator Sxy"
                     /protein_id="HGU4547109.1"
                     /translation="MKSLSYKRIYKSQEYLATLGTIEYRSLFGSYSLTVDDTVFAMVS
                     DGELYLRACEQSAQYCVKHPPVWLTYKKCGRSVTLNYYRVDESLWRNQLKLVRLSKYS
                     LDAALKEKSTRNTRERLKDLPNMSFHLEAILGEVGIKDVRALRILGAKMCWLRLRQQN
                     SLVTEKILFMLEGAIIGIHEAALPVARRQELAEWADSLTPKQEFPAELE"
     gene            complement(15896..18058)
                     /gene="yccS"
                     /locus_tag="ACHOSC_RS05655"
                     /old_locus_tag="pgaptmp_001131"
     CDS             complement(15896..18058)
                     /gene="yccS"
                     /locus_tag="ACHOSC_RS05655"
                     /old_locus_tag="pgaptmp_001131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309071.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YccS family putative transporter"
                     /protein_id="HGU4547110.1"
                     /translation="MAFMLSPLLKRYTWNSAWLYYARIFIALCGTTAFPWWLGDVKLT
                     IPLTLGMVAAALTDLDDRLAGRLRNLIITLFCFFIASASVELLFPWPWLFAIGLTLST
                     SGFILLGGLGQRYATIAFGALLIAIYTMLGTSLYEHWYQQPMYLLAGAVWYNVLTLIG
                     HLLFPVRPLQDNLARCYEQLARYLELKSRMFDPDIEDESQAPLYDLALANGLLMATLN
                     QTKLSLLTRLRGDRGQRGTRRTLHYYFVAQDIHERASSSHIQYQTLREHFRHSDVLFR
                     FQRLMSMQGQACQQLSRCILLRQPYQHDPHFERAFTHIDAALERMRDNGAPADLLKTL
                     GFLLNNLRAIDAQLATIESEQAQALPHNNDENELADDSPHGLSDIWLRLSRHFTPESA
                     LFRHAVRMSLVLCFGYAIIQITGMHHGYWILLTSLFVCQPNYNATRHRLKLRIIGTLV
                     GIAIGIPVLWFVPSLEGQLVLLVITGVLFFAFRNVQYAHATMFITLLVLLCFNLLGEG
                     FEVALPRVIDTLIGCAIAWAAVSYIWPDWQFRNLPRMLERATEANCRYLDAILEQYHQ
                     GRDNRLAYRIARRDAHNRDAELASVVSNMSSEPNVTPQIREAAFRLLCLNHTFTSYIS
                     ALGAHREQLTNPEILAFLDDAVCYVDDALHHQPADEERVNEALASLKQRMQQLEPRAD
                     SKEPLVVQQVGLLIALLPEIGRLQRQITQVPQETPVSA"
     gene            complement(18068..18514)
                     /gene="yccF"
                     /locus_tag="ACHOSC_RS05660"
                     /old_locus_tag="pgaptmp_001132"
     CDS             complement(18068..18514)
                     /gene="yccF"
                     /locus_tag="ACHOSC_RS05660"
                     /old_locus_tag="pgaptmp_001132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YccF domain-containing protein"
                     /protein_id="HGU4547111.1"
                     /translation="MRTVLNILNFVLGGFATTLGWLLATLVSIVLIFTLPLTRSCWEI
                     TKLSLVPYGNEAIHVDELNPAGKNVLLNTGGTVLNIFWLIFFGWWLCLMHIATGIAQC
                     ISIIGIPVGIANFKIAAIALWPVGRRVVSVETAQAAREANARRRFE"
     gene            18637..20691
                     /gene="helD"
                     /locus_tag="ACHOSC_RS05665"
                     /old_locus_tag="pgaptmp_001133"
     CDS             18637..20691
                     /gene="helD"
                     /locus_tag="ACHOSC_RS05665"
                     /old_locus_tag="pgaptmp_001133"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase IV"
                     /protein_id="HGU4547112.1"
                     /translation="MELKATTLGKRLAQHPYDRAVILNAGIKVSGDRHEYLIPFNQLL
                     AIHCKRGLVWGELEFVLPDEKVVRLHGTEWGETQRFYHHLDAHWRRWSGEMSEIASGV
                     LRQQLDLIATRTGENKWLTREQTSGVQQQIRQALSALPLPVNRLEEFDNCREAWRKCQ
                     AWLKDIESARLQHNQAYTEAMLTEYADFFRQVESSPLNPAQARAVVNGEHSLLVLAGA
                     GSGKTSVLVARAGWLLARGEASPEQILLLAFGRKAAEEMDERIRERLHTEDITARTFH
                     ALALHIIQQGSKKVPIVSKLENDTAARHELFIAEWRKQCSEKKAQAKGWRQWLTEEMQ
                     WSVPEGNFWDDEKLQRRLASRLDRWVSLMRMHGGAQAEMIASAPEEIRDLFSKRIKLM
                     APLLKAWKGALKAENAVDFSGLIHQAIVILEKGRFISPWKHILVDEFQDISPQRAALL
                     AALRKQNSQTTLFAVGDDWQAIYRFSGAQMSLTTAFHENFGEGDRCDLDTTYRFNSRI
                     GEVANRFIQQNPGQLKKPLNSLTNGDEKAVTLLDESQLDALLDKLSGYAKPEERILIL
                     ARYHHMRPASLEKAATRWPKLQIDFMTIHASKGQQADYVIIVGLQEGSDGFPAAARES
                     IMEEALLPPVEDFPDAEERRLMYVALTRARHRVWALFNKENPSPFVEILKNLDVPVAR
                     KP"
     gene            complement(20723..21181)
                     /gene="mgsA"
                     /locus_tag="ACHOSC_RS05670"
                     /old_locus_tag="pgaptmp_001134"
     CDS             complement(20723..21181)
                     /gene="mgsA"
                     /locus_tag="ACHOSC_RS05670"
                     /old_locus_tag="pgaptmp_001134"
                     /EC_number="4.2.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415483.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008929 - methylglyoxal synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006007 - glucose catabolic process
                     [Evidence IEA];
                     GO_process: GO:0019242 - methylglyoxal biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylglyoxal synthase"
                     /protein_id="HGU4547113.1"
                     /translation="MELTTRTLPARKHIALVAHDHCKQMLMSWVERHQPLLEQHVLYA
                     TGTTGNLISRATGMNVNAMLSGPMGGDQQVGALISEGKIDVLIFFWDPLNAVPHDPDV
                     KALLRLATVWNIPVATNVATADFIIQSPHFNDAVDILIPDYQRYLADRLK"
     gene            complement(21277..21939)
                     /gene="yccT"
                     /locus_tag="ACHOSC_RS05675"
                     /old_locus_tag="pgaptmp_001135"
     CDS             complement(21277..21939)
                     /gene="yccT"
                     /locus_tag="ACHOSC_RS05675"
                     /old_locus_tag="pgaptmp_001135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2057 family protein"
                     /protein_id="HGU4547114.1"
                     /translation="MKTGIVTTLIALCLPVSVFATTLRLSTDVDLLVLDGKKVSSSLL
                     RGADSIELDNGPHQLVFRVEKTIHLSNSEERLYISPPLVVSFNTQLINQVNFRLPRLE
                     NEREANHFDAAPRLELLDGDATPIPVKLDILAITSTAKTIDYEVEVERYNKSAKRASL
                     PQFATMMADDSTLLSGVSELDAIPPQSQVLTEQRLKYWFKLADPQTRNTFLQWAEKQP
                     SS"
     gene            22112..22525
                     /gene="yccU"
                     /locus_tag="ACHOSC_RS05680"
                     /old_locus_tag="pgaptmp_001136"
     CDS             22112..22525
                     /gene="yccU"
                     /locus_tag="ACHOSC_RS05680"
                     /old_locus_tag="pgaptmp_001136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0120225 - coenzyme A binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-binding protein"
                     /protein_id="HGU4547115.1"
                     /translation="MKETDIAGILTSTHTIALVGASDKPDRPSYRVMKYLLDQGYHVI
                     PVSPKVAGKTLLGQQGYGTLADVPEKVDMVDVFRNSEAAWGVAQEAIAIGAKTLWMQL
                     GVINEQAAVLARDAGLNVVMDRCPAIEIPRLGLAK"
     gene            complement(22570..22887)
                     /gene="hspQ"
                     /locus_tag="ACHOSC_RS05685"
                     /old_locus_tag="pgaptmp_001137"
     CDS             complement(22570..22887)
                     /gene="hspQ"
                     /locus_tag="ACHOSC_RS05685"
                     /old_locus_tag="pgaptmp_001137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heat shock protein HspQ"
                     /protein_id="HGU4547116.1"
                     /translation="MIASKFGIGQQVRHSLLGYLGVVVDIDPVYSLSEPSPDELAVND
                     ELRAAPWYHVVMEDDNGLPVHTYLAEAQLSSELQDEHPEQPSMDELAQTIRKQLQAPR
                     LRN"
     gene            complement(22945..24135)
                     /gene="rlmI"
                     /locus_tag="ACHOSC_RS05690"
                     /old_locus_tag="pgaptmp_001138"
     CDS             complement(22945..24135)
                     /gene="rlmI"
                     /locus_tag="ACHOSC_RS05690"
                     /old_locus_tag="pgaptmp_001138"
                     /EC_number="2.1.1.191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415487.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0008649 - rRNA methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (cytosine(1962)-C(5))-methyltransferase
                     RlmI"
                     /protein_id="HGU4547117.1"
                     /translation="MSVRLVLAKGREKSLLRRHPWVFSGAVARMEGKASLGETIDIVD
                     HQGKWLARGAYSPASQIRARVWTFDPSESIDIAFFSRRLQQAQKWRDWLAQKDGLDSY
                     RLIAGESDGLPGITIDRFGNFLVLQLLSAGAEYQRAALVSALQTLYPECAIYDRSDVA
                     VRKKEGMELTQGLVTGELPPALLPIEEHGMKLLVDIQHGHKTGYYLDQRDSRLATRRY
                     VENKRVLNCFSYTGGFAVSALMGGCSQVVSVDTSQEALDIARQNVELNKLDLSKAEFV
                     RDDVFKLLRTYRDRGEIFDVIVMDPPKFVENKSQLMGACRGYKDINMLAIQLLNEGGI
                     LLTFSCSGLMTSDLFQKIIADAAIDAGRDVQFIEQFRQAADHPVIATYPEGLYLKGFA
                     CRVM"
     gene            24230..24508
                     /gene="yccX"
                     /locus_tag="ACHOSC_RS05695"
                     /old_locus_tag="pgaptmp_001139"
     CDS             24230..24508
                     /gene="yccX"
                     /locus_tag="ACHOSC_RS05695"
                     /old_locus_tag="pgaptmp_001139"
                     /EC_number="3.6.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003998 - acylphosphatase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acylphosphatase"
                     /protein_id="HGU4547118.1"
                     /translation="MSKVCIIAWVYGRVQGVGFRYTTQYEAKRLGLTGYAKNLDDGSV
                     EVVACGEEGQVEKLMLWLKSGGPRSARVERVLSEPHHPSGELTDFRIR"
     gene            complement(24505..24834)
                     /gene="tusE"
                     /locus_tag="ACHOSC_RS05700"
                     /old_locus_tag="pgaptmp_001140"
     CDS             complement(24505..24834)
                     /gene="tusE"
                     /locus_tag="ACHOSC_RS05700"
                     /old_locus_tag="pgaptmp_001140"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309080.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase TusE"
                     /protein_id="HGU4547119.1"
                     /translation="MLIFEGKEIETDTEGYLKESSQWSEPLAVVIAENEGISLSPEHW
                     EVVRFVRDFYLEFNTSPAIRMLVKAMANKFGEEKGNSRYLYRLFPKGPAKQATKIAGL
                     PKPVKCI"
     gene            complement(24925..25584)
                     /gene="yccA"
                     /locus_tag="ACHOSC_RS05705"
                     /old_locus_tag="pgaptmp_001141"
     CDS             complement(24925..25584)
                     /gene="yccA"
                     /locus_tag="ACHOSC_RS05705"
                     /old_locus_tag="pgaptmp_001141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000375124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsH protease modulator YccA"
                     /protein_id="HGU4547120.1"
                     /translation="MDRIVSSSHDRTSLLSTHKVLRNTYFLLSLTLAFSAITATASTV
                     LMLPSPGLILTLVGMYGLMFLTYKTANKPTGIISAFAFTGFLGYILGPILNTYLSAGM
                     GDVIAMALGGTALVFFCCSAYVLTTRKDMSFLGGMLMAGIVVVLIGMVANIFLQLPAL
                     HLAISAVFILISSGAILFETSNIVHGGETNYIRATVSLYVSLYNIFVSLLSILGFASR
                     D"
     gene            complement(25992..27011)
                     /locus_tag="ACHOSC_RS05710"
                     /old_locus_tag="pgaptmp_001142"
     CDS             complement(25992..27011)
                     /locus_tag="ACHOSC_RS05710"
                     /old_locus_tag="pgaptmp_001142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HGU4547121.1"
                     /translation="MGRPRKNKKDNVLPPRVRSNGYSYVWKPEGSTRSIGLGRVRKTS
                     VAKVWQNYELEKAKLHNIMTVAKLWHMFMDSPAFTELAPRTQKDYRQHQKALLMVFGK
                     VLADNVKTEQVRIFMDKRGLESKTQANHELASLSRVYGWGYERGYVKNNPCKGVRKFS
                     LKARTVYITDEQYAAIYAEAIPQLRIAMEISYLCAARLGDVLELKWQDIMDKGIYIEQ
                     NKTGTKQIKEWSPRLRTAIQLARNVSSCTCEYVINTTKGGKVIAKTLNNWWNQAKRAA
                     EQKVGVPFGCNFHDIKAKGISDYEGSSRDKQIFSGHKTENQVLIYDRKTKITPTLDLP
                     LVVSK"
     gene            complement(26989..27231)
                     /locus_tag="ACHOSC_RS05715"
                     /old_locus_tag="pgaptmp_001143"
     CDS             complement(26989..27231)
                     /locus_tag="ACHOSC_RS05715"
                     /old_locus_tag="pgaptmp_001143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_303789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4224 domain-containing protein"
                     /protein_id="HGU4547122.1"
                     /translation="MYELTLSPAEIKEITKYERYTKQQHQLRLHGIPFVIGPKNEPIV
                     LRRDIPHGLTTMPKTSELVSAEPDFEALNNGKTKKK"
     gene            complement(27299..>28426)
                     /locus_tag="ACHOSC_RS05720"
                     /old_locus_tag="pgaptmp_001144"
     CDS             complement(27299..>28426)
                     /locus_tag="ACHOSC_RS05720"
                     /old_locus_tag="pgaptmp_001144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3'-5' exoribonuclease"
                     /protein_id="HGU4547123.1"
                     /translation="VDTAEDSVQPEPVKVTADEVNKIMQAANISQPDADKLLAASRGE
                     FVEGISDPNDPKWVKGIETCDSVNQNQQESEQNDQKTEQNSPNAQQNEPETKQEPEKA
                     CTACGQTGGGSCPDCGAVISDATYQEIFDEENQPEVQENDPEEMEGTAHQHKENTGGN
                     QHHASDSETGEASDPLIKANGHHNLTSTSRAGIHLMIDLETMGKNPDAPIISIGAIFF
                     DPQTGEMGPEFSKTIDLETAGGVIDRDTIKWWLKQSREAQSAILTDEIPLDDALLQLR
                     EFIDENSGEFFVQVWGNGATFDNVILRRSYERQGIPCPWRYTNDRDVRTMVALGLVMD
                     FDARTTIPFEGERHNALNDARYQAKYVSAIWQKLLPSQADF"
BASE COUNT         7155 a         6934 c         7389 g         6948 t
ORIGIN      
        1 ttgttttata ttcatcgctg ttgcttattt agacattttg tacttttatt gttgaggtta
       61 ttaagcgaag cgacaatgga ttgtgttgct gcggttttat agggtagggg agaggcagat
      121 gcgaattaaa ccagacgata actggcgttg gtattacgat gaagagcacg atcgaatgat
      181 gctcgattta gccaatggta tgctatttcg ctcacgtttt gcgcgcaaga tgttgactcc
      241 agatgccttt tctcccgcag gcttttgcgt tgacgacgcc gcgctctatt tctcttttga
      301 agaaaagtgc cgcgatttta atttatctaa agaacaaaaa gccgaactgg tgctgaatgc
      361 actggtagca attcgctatc tcaaaccgca aatgccgaaa agctggcatt ttgtttccca
      421 tggtgaaatg tgggtgccca tgccgggcga tgcggcctgc gtttggttga gcgatactca
      481 cgagcaggtc aatttgctgg tcgttgaatc tggcgaaaat gccgcactat gcctgctggc
      541 acaaccctgc gttgttattg cgggtcgcgc gatgcagttg ggcgacgcca ttaaaatcat
      601 gaacgacagg ctgaaaccgc aggttaatgt tgacagcttc agcctcgaac aggcagtcta
      661 acgcgccaac ttaagtgcag tcttcggtac acagctacag caaagaatgg tgccatcatc
      721 gcccattgct gattttttta gcggcgtgac ttcgccttct aaaagctgaa cacggcaact
      781 tccacaaatg cccgcgcggc aagaataagg gatacgaatt ccctgatttt ctaattgctc
      841 cagcaacacc tgttggttat ttccacgaaa tgcctgtccc tgccagtcaa tatctacatt
      901 tgcgtccggt tgttgcgtga tgttggcggt atcatcagcg gcagctgcgc cgtaaatttt
      961 tgccggagcc gttgccagaa tttccacctc atcgccaacg cgaatcacgc cgctattacg
     1021 ggcaattaaa ttctgaccaa aatcgacatc gccgttatcc tgggcagtgc ggaaagattg
     1081 caatgttttt aatggttcgc ctgccggatg tttttgccct ttttctgggc tgacggtggt
     1141 gaaaatacag cggctacaag gtttaaccac atcaaacacc acatcaccaa tgcgaatcac
     1201 tttccagcga tcttcttccc aggctgacgc gccggaaacc accagattgg ggcggaattg
     1261 ctccattttt acactggccg gacaacgttg ttggagatca cgtaacgagg cttcgttagc
     1321 aagaaggtaa ggatagccat cagcaaatga cagaggtaca gtgttgtggc gtttcacgcg
     1381 ccgggtcatt tgtggcccca cccagcgtaa ttgcacttcg cgggagaaaa atccacttag
     1441 ccatttgttg atcgcgcctg gcgcaattcg cgcggtaaaa tgtgtgcccc aaacttcggt
     1501 tggtgcgtct tgtgtggcga aatcagcaaa acgaacatat gcgctactgc catctggtgc
     1561 ggtgagatgc aagccatcat gcacaggtga aggagtaaac cgtaccattt gcggaaactg
     1621 gcgagcagta ataaacgtac catcaggttc cgtgatcata aagatgcgat cgaaggccag
     1681 accactcaca tctgccaggg tatgtgtaag accaatgccg cgcatcgatt taacaggatg
     1741 aataaaaagc cgggttaatg tcgccacggt gcggtcctca aatgaaaata agccctcaac
     1801 tttatgacat gacgcgctta ttagctataa tgcgcaacaa ttttcttagc gcctgtcccg
     1861 ttaggctatt ttatttgtca gtttggccca gggaagtgct cgaaatcctc acgtacttgt
     1921 gtacgctccg gtttctccgc gctgtccatg tccagactga ctgcaacaat tacgcctact
     1981 gcggtaggct cttagagtaa aagtgacgat atgaattctc tgtttgccag tacggcccgt
     2041 gggctggaag agctgttaaa aactgaactg gaaaacctgg gggccgttga atgccaggtg
     2101 gttcagggtg gggtccattt caagggcgac acacggcttg tttaccagag cctgatgtgg
     2161 agccgcctgg cctcgcgtat tatgttgccg ctgggcgagt gtaaggttta cagcgattta
     2221 gacctctatc tcggtgttca ggcgatcaac tggacagaga tgtttaatcc tggcgcgacc
     2281 ttcgctgtcc acttcagtgg tttgaatgac accatccgca acagtcagta cggcgcgatg
     2341 aaagtgaaag acgcgatcgt cgatgctttc acgcggaaaa atctgccgcg tccaaatgtt
     2401 gatcgcgatg cgccggatat ccgcgttaac gtctggctgc ataaagaaac cgccagtatc
     2461 gctcttgatc tgagtggtga tggtttacat ctgcgtggct atcgcgatcg tgctggtatt
     2521 gcgccgatca aagaaaccct ggcagccgct attgtgatgc gatccggctg gcagccagga
     2581 acaccgctgc tcgatccgat gtgtggttcc ggtacgttgc tgattgaagc ggcgatgctg
     2641 gcgaccgatc gcgcaccagg cttgcaccgt gggcgttggg gctttagcgg ctgggcgcag
     2701 catgatgaag ctatctggca ggaagtgaaa gcggaagcgc aaactcgcgc ccgtaaaggc
     2761 ctggctgagt atagctctca tttttacggt tcggacagcg acgcacgggt gattcaacgt
     2821 gcacgcacta acgcccgtct tgcggggatt ggtgaactga tcacctttga ggtgaaagat
     2881 gtcgcgcaac tgaccaatcc gctgccgaaa gggccgtacg gcacagtgtt gagcaacccg
     2941 ccatacgggg aacgtctgga cagcgaaccg gcgctgattg cgctgcatag cctgctgggt
     3001 cggatcatga aaaaccagtt tggtggctgg aatctctctt tgtttagtgc ctcgccggat
     3061 ctgctaagct gcttgcagct gcgtgcagac aaacagtaca aggcgaaaaa cggcccgctg
     3121 gactgcgtac agaaaaatta ccacgttgcc gaaagcactc cagacagcaa accggcgatg
     3181 gtagcggaag actacgccaa ccgcctgcgt aagaacctca aaaaattcga gaagtgggct
     3241 cgccaggaag gaattgaatg ttaccgcctg tatgacgccg atctaccgga atataacgtt
     3301 gccgttgacc gttatgccga ctgggtggtg gtgcaggagt atgcgccgcc aaaaactatt
     3361 gatgctcaca aagcgcgtca gcgtctgttc gatattatcg ctgcaaccat ttcggtactg
     3421 gggattgcgc caaacaaact ggtgctgaaa acccgtgaac gccagaaggg caaaaatcag
     3481 taccagaaac tgggcgagaa gggcgagttt cttgaagtta ccgaatataa cgctcacttg
     3541 ttggtgaacc tgacggatta cctcgatacc ggtctgtttc tcgatcaccg tatcgcccgt
     3601 cgtatgctcg gccagatgag caaaggcaaa gatttcctca acctgttctc ttataccggc
     3661 agcgccaccg tgcatgcggg attaggcggt gcacgcagca ccaccaccgt ggatatgtcg
     3721 cgtacttatc tggagtgggc agaacgcaac ctgcgtctga atggcctgac cgggcgtgcg
     3781 catcgcctga ttcaggccga ttgcctggcg tggctgcgtg aggcaaatga acagttcgat
     3841 ctgatcttta tcgatccgcc aaccttctct aactcaaaac gaatggaaga tgcgtttgat
     3901 gttcagcgcg atcatctggc gctgatgaaa gatttgaaac gtctgctgcg tgcaggtggg
     3961 acgatcatgt tctcgaacaa caaacgtggc ttccgtatgg atctcggcgg cctggcaaaa
     4021 ctgggactga aagcacaaga aattacgcaa aaaacgctct cccaggattt cgcccgtaac
     4081 cgccagatcc acaactgctg gctgattacc gcagcctgaa aggaatagta atgtcattaa
     4141 tcagtatgca tggcgcctgg ctgtcgttca gcgacgcgcc gcttctcgat aacgcagaac
     4201 tgcatatcga agataatgaa cgtgtttgtc tggtaggccg caacggcgca ggcaaatcga
     4261 cgttaatgaa aatcctcaac cgtgaacaag ggctggatga cggtcgcatt atttacgagc
     4321 aagatttgat tgtagctcgt ctgcaacagg atccgccgcg taacgttgag ggtagcgttt
     4381 atgatttcgt tgccgaaggc attgaagaac aagcggaata tctgaaacgc tatcacgata
     4441 tttcgcgcct ggtgatgaac gacccgagcg agaaaaatct caacgaactg gcgaaggttc
     4501 aggaacagct ggatcaccac aacttgtggc agctggaaaa ccgcatcaac gaagtgctgg
     4561 cgcaactggg gttagatcct aatgttgcgc tgtcgtcgct ttccggcggc tggttgcgta
     4621 aagcggcatt aggacgcgcg ctggtgagca atccgcgcgt attgctgctg gacgaaccga
     4681 ctaaccatct ggatatcgaa accatcgact ggctggaagg gttccttaaa accttcaatg
     4741 ggacgattat tttcatctcc cacgaccgtt cgtttatccg caatatggcg acgcgcattg
     4801 ttgatctcga tcgcggcaag ctggtgacct atccggggaa ttacgaccag tacctgcagg
     4861 aaaaagaaga agccctgcgc gtggaagaac tacaaaatgc cgagttcgat cgcaaactgg
     4921 cgcaggaaga agtgtggatt cgccagggga tcaaagcacg ccgtacccgt aatgaaggcc
     4981 gcgtacgcgc cctgaaagca atgcgtcgcg agcgtggtga acgtcgcgaa gtgatgggta
     5041 ccgcaaagat gcaggtggaa gaggccagcc gctccggtaa gatcgttttc gaaatggaag
     5101 acgtttgcta ccaggttgac ggtaagcaac tggtgaaaga tttttcggcc caggttctac
     5161 gtggcgacaa aattgccctg attggtccga atgggtgcgg caaaaccacg ctgctgaaac
     5221 tgatgctcgg tcagcttcaa gcggacagcg ggcgtattca cgttggcacc aaactggaag
     5281 tggcttattt cgatcagcac cgcgcggaac tggatcccga taaaacggtg atggataacc
     5341 ttgccgaagg taagcaagag gtgatggtta acggcaagcc acgccacgta ttgggctatt
     5401 tgcaggactt tctgttccat ccgaaacggg cgatgacgcc ggtacgtgcg ctttctggcg
     5461 gtgagcggaa ccgcttgctg ctggcgcgtt tgttcctcaa accaagcaac ttattgattc
     5521 ttgacgaacc gaccaacgat cttgatgtcg aaacgctgga actgctggaa gaactgatcg
     5581 acagctatca gggcacggta ttgctggtta gccacgatcg tcagtttgtc gataacaccg
     5641 ttacagaatg ttggatcttc gaaggcggcg gtaaaattgg tcgttatgtc ggcggttatc
     5701 atgatgcccg tggtcagcaa gagcagtatg tggcgctcaa acagcctgcg gtgaaaaaaa
     5761 ccgaagaagc cgctgcggca aaagcagaaa ctgtaaaacg cagcagtagc aaactaagct
     5821 ataaattgca gcgcgaactg gagcagctac cgcaattgct cgaagatctg gaggcgaagc
     5881 tggaagccct acagacgcag gtggcggatg cttccttctt cagtcagcca catgagcaga
     5941 cacaaaaagt gctcgccgat atggctgctg cagagcagga gctggagcaa gcctttgaac
     6001 gctgggagta tcttgaagcg ttaaaaaatg gtggctgatc gcaaaatagt cgatttaaac
     6061 agagacagct gacaacggta aaattgcctg atgaactggg tttatcaggc atgaaaacca
     6121 acactttgtt agtcatctgt aaaatagcgc atcattaagg agtaccaatg tgcgaacatc
     6181 atcatgccgc gaagcacatc ctgtgctcgc agtgtgacat gctggtggcg ttaccgcgcc
     6241 ttgagcatgg tcagaaagcg gcatgtcccc ggtgtggcac aacgttaacc gtggcgtggg
     6301 atgcccctcg gcagcgtccc accgcctatg cgttggctgc actgttcatg ctgttgctgt
     6361 ccaacttgtt tccttttgtg aatatgaacg ttgcaggagt taccagtgaa attacattac
     6421 tggaaattcc cggcgtgctt ttttctgagg actacgccag cctcggcacc tttttcctgt
     6481 tgtttgtgca actggttccc gcgttttgtc tgataaccat tctgttactg gtgaatcgcg
     6541 cggaattacc ggtccgttta aaagagcaac tggcacgggt gctttttcaa ctcaaaacct
     6601 ggggaatggc ggagattttc ctcgctggtg tgctggtcag tttcgttaaa ctgatggctt
     6661 acggcagcat tggggtaggc agcagctttc tcccctggtg tttattttgt gtcctgcaac
     6721 tgcgcgcttt tcagtgcgtt gatcgtcgct ggttatggga cgacatcgcc ccgatgccag
     6781 aactgcgcca gccgctaaaa ccaggcgtca cggggatacg tcaggggctg cgttcgtgct
     6841 cctgttgtac ggcaatcctt cctgctgatg aacccgtgtg cccgcgttgt agtaccaaag
     6901 ggtacgttcg gcgtagaaac agcctgcagt ggacactcgc gctgcttgta acgtccatca
     6961 tgctgtatct tccggctaat attttgccca tcatggtgac ggatttatta ggctcgaaga
     7021 tgccgtcgac gattctcgct ggggtcattc tgttatggag cgaaggctct tatcccgtcg
     7081 ctgcggtgat ctttctggcc agtattatgg tgccaacgtt aaagatgatc gccatcgcgt
     7141 ggctgtgttg ggatgccaaa gggcatggca agcgcgacag tgaaagaatg catttgattt
     7201 atgaagttgt tgagtttgta ggccgctggt cgatgattga cgttttcgtt atcgcggtgc
     7261 tctcggcgct ggtgcgtatg ggaggtttaa tgagtattta tccggcaatg ggtgcattaa
     7321 tgtttgcttt agtcgtcata atgacaatgt tttctgctat gacgtttgac ccgcgtttgt
     7381 cgtgggatcg tcaacctgaa tcagagcatg aggagtcctg acagtatgga atctaataat
     7441 ggggaagcca aaatccagaa agtgaagaac tggtctcccg tgtggatatt tcctatcgtc
     7501 acggcgctca ttggggcctg ggttcttttt tatcattaca gccatcaggg accggaagtg
     7561 accctgatca ccgcgaatgc ggaaggaatt gaaggtggca aaaccaccat taaaagccgt
     7621 agcgttgacg tcggcgtggt tgaaagcgcc acactggctg atgatttgac gcacgttgaa
     7681 atcaaagcgc ggctgaattc cggtatggaa aaattgctgc ataaagacac cgtcttttgg
     7741 gtggtgaaac cgcagattgg tcgagaaggg attagcggtc tgggaaccct gctgtctggg
     7801 gtttatatcg aactgcagcc aggcgcgaaa ggcagcaaaa tggataaata cgatttgctg
     7861 gactcgccac cgttggcccc gcctgatgcg aaaggtatcc gtgtgattct cgatagcaaa
     7921 aaagccgggc agctctcgcc aggagatccg gtgctgttcc gtggctatcg ggtaggttcg
     7981 gttgaaacca gcaccttcga tacacaaaaa cgcaatatca gctatcaact gttcatcaat
     8041 gcaccttatg accgactggt gaccaacaat gttcgcttct ggaaagatag tggcattgcg
     8101 gttgatctga cgtcagcagg gatgcgtgtg gagatgggct cattgacaac gctgctgagt
     8161 ggcggtgtca gctttgatgt gccggaaggt ctggatttag ggcagccagt ggcaccgaaa
     8221 acagctttcg ttttgtatga tgatcagaag agcattcagg attcgttgta caccgatcac
     8281 attgattatc tgatgttctt taaagattcg gtacgcggtc tgcaaccggg agctccggta
     8341 gagttccggg gtattcgcct gggtaccgta agcaaagtgc cattctttgc gccgaatatg
     8401 cgtcagacat ttaacgatga ttaccgtatt ccggtactga ttcgtatcga gccagagcgg
     8461 ctgaaaatgc agcttggcga aaatgcggat gttgttgagc accttggcga attgttgaaa
     8521 cgtggtttac gcggatcgct gaaaaccgga aacctggtca ctggtgcact gtatgttgat
     8581 ctcgatttct atccaaatac gcctgcaata accggtattc gtgaatttaa tggttatcag
     8641 attatcccga ccgttagcgg cggcctggcg caaatccagc aacgactgat ggaagcgttg
     8701 gataagatca acaaactgcc attgaatccg atgattgaac aggcaaccag tacgctttct
     8761 gaaagtcagc gcacaatgaa aaacctgcaa acgacgctgg atagcatgaa caagatcctc
     8821 gctagccagt cgatgcagca gttgccgacg gatatgcagt caacgttgcg tgaattgaat
     8881 cgcagcatgc agggcttcca gcctggctcc gcagcctaca acaagatggt ggcggatatg
     8941 cagcgccttg atcaggtgtt gcgagaactg caaccggtgc tgaaaacgct caatgagaag
     9001 agtaacgcgc tggtatttga agcgaaggac aaaaaagatc cagagccgaa gagggcgaaa
     9061 caatgaaaaa gtggctagtg acgattgcag cactgtggct ggccggatgc agctccggcg
     9121 aaattaataa aaactattac cagttacctg tggtgcagag cggtacacaa agtaccgcca
     9181 gccagggcaa tcgtctgtta tgggtagagc aggtcactgt tcctgactat ctggcgggga
     9241 atggtgtggt ttatcaaacc agtgatgtga agtatgtgat tgccaataac aacttgtggg
     9301 ccagcccgtt ggatcaacag ttgcgcaaca ccctggttgc caacctgagt acgcaactgc
     9361 ccggctgggt ggttgcctcc cagcctctgg gaagcgccca ggacacgctc aatgttaccg
     9421 tcacggagtt taacggtcgc tatgatggca aggtcattgt cagtggtgag tggctgttga
     9481 accaccaggg acaactgatc aaacgtccgt tccgtctgga aggggtgcaa actcaggatg
     9541 gttacgatga gatggttaaa gtactggcca gtgcctggag tcaggaagcc gcttctattg
     9601 cgcaagagat aaagcgtcta ccttaattat aaagatttgt aaatataacc gcctccggta
     9661 tgttgcctga ggcggttttt ttgtctctaa cgtgcggaaa aagttgttcc tcttcacatt
     9721 ttttgtacaa ccggcatacc cgtgtagctc acaaatatga cagtggcgtg aattttgcgc
     9781 attgacggca gttatgattc gcggtattgc ttaactgtga ttgcacattt agtaatcact
     9841 gttttctttt ccaccagaaa ccagtatgag ggaaacgagg catgaagaga caaaaacgag
     9901 atcgcctgga acgggcacat caacgtggtt atcaggccgg catcgccgga cgctcaaaag
     9961 aaatgtgtcc ctatcagacg ctgaatcaaa ggtcacaatg gctgggaggc tggcgagaag
    10021 ccatggcgga cagggtagta atggcctgat tctgtctctt taaaaagaaa cctccgcatt
    10081 gcggaggttt cgccttttga tactctgtct gattataatc agaaggcaga cgtatcctgg
    10141 aacagaccga ctttcaggtc gctggcggta tagatcagac gaccatcaac cagcacttcg
    10201 ccatccgcca ggcccataat cagacgacgg ttaacaatgc gtttaaagtg aatacggtag
    10261 gtcacttttt tcgctgtcgg cagtacctga ccagtgaatt tcacttcgcc aacgcccagc
    10321 gcgcggcctt taccttcgcc gcccagccag ccgaggtaga accctaccag ctgccacatt
    10381 gcgtccaggc ccaggcatcc cggcataacc ggatcgccaa taaagtggca tccgaagaac
    10441 cacagatccg gattgatatc cagttctgct tcaacatacc ctttgtcgaa gttaccaccc
    10501 gtttcggtca ttttgaccac acggtccatc atcagcatgt tcggtgctgg caattgcggg
    10561 cctttagcgc caaacagttc accgcgacca gaggcaagaa ggtcttcttt tgtataggat
    10621 tcgcgtttat ctaccatgtt ctctgtaagc cttattttat tgaagcacgc aggatagcta
    10681 acacgtgtac gctgaacaag tccgatcagt tcggaataaa ccagttcagc caacgtaatg
    10741 gccatggaaa acggtgacgt ccttcctgtt gcgatgcttg cgcgatacgt tcctggatgg
    10801 tttgcatcag cgtcgtttgg ccttcgccat cccacaccag atttaataat aacggcagtg
    10861 cgtcagtcac atcgtctact gcccagatgg tgaatttgcc ttcttctacc gctttcacca
    10921 gttcactgtg aagacttaaa tggcgaacgt tagctgtggg gataatgaca ccttgtttcc
    10981 cggttaactc acgttgctgg caaatagcaa agaagccttc gattttctca tttaaaccac
    11041 cgaccggctg ggcgcgaccg aactgatcga ctgaacctgt gatagcgata ctctgattca
    11101 ccggcacatc ggcgagggcg cttatcaggg cgcagagttc agccatcgag gcactatctc
    11161 catcaacttc actgtatgac tgctcaaatg tcagcgatgc tgagaagggg atctgttgct
    11221 caagctgtag ttccgacatc aggaacgctt gcatgatcat catccctttc gcatggatat
    11281 tgccgccaag ctccgctttg cgttcgatgt cggtgaattc accatcgcca atatgcacaa
    11341 cgcagctaat gcgagaaggt tcgccaaaag cgcgtggatg acccggaaat tcaatgaccg
    11401 aaagggcgtt aatttgcccg atgcgttcgc cttcggtttc aatcaggatt tgctcctgaa
    11461 ggatctcatc ctgcatacgt tcagcgagga aaccttcgcg ccattcacgc tgctgcagca
    11521 ttaagtttag ctgctcgccg gagaaggtgt cgccatcaca cagggaggcg acctctttac
    11581 actggcggag gatccactgc gggctaagcg gaagtgtttc ttgttcaccg gtgtagcgtg
    11641 ctgcttcgcg gataagtacc ggccaggcat ccgctcccgg tgcaggtaag tgattatgtc
    11701 tggcggtaaa tgtcacccag cgacaccact gggttactga ctctgcatcg acaatctgca
    11761 gagtatcttc aaattcgcta taaatagcct gctctgaaag ctctggctcc atctcctgga
    11821 aatcagacaa tgattcgcgt tcgcctacca gaatgacttt cagcttcaat ggcatcgaag
    11881 gcacagagac ggggagaggg cgcgactcat caaacgcaac ccagtcaaaa cgctcgcggt
    11941 taacgatatt tttcagccgc atccacagca gaggttgcgc cagcagtgta cgcaaagaga
    12001 tgatgagaat accgccattt gcctgatgca ccagaccagg ctgcagggta atgtcgccat
    12061 taaactggcg caggcagcca aagagttgct ccgcttctac ccagtcggca gcgacaactt
    12121 gcgttaaagt cgcaaaatta tcatctgcac tcactgcgtg acgtaagcgg atggagtggc
    12181 cggaaacctc atagtgaccg cccaccagtt ggcctgcatc gctttgtagc gtacgcgcgg
    12241 cattggcaat aagattcaga tactcagact cttccggggc cttcgccagc ataaaagagg
    12301 aggatgctcg cgtatgcagc aactgctcca gtgcgaattg cagtcgcggt tgagtatcac
    12361 tgaataaagg atcgttttcg tcaatcaaat gtggctgagc aaatatttcc tgatagctat
    12421 cggtatcagg aaccaggtca cgccatgcaa gtttcgtaat ggtcaaagtt gatgtttttt
    12481 agtctgttgt caaagccgcg attataccgt aaccggcact acagcacacg tagaaagcac
    12541 cgacaatact cctggcatgg gcgttaaagc tcacaggatg gagattcttt tcttcactgg
    12601 cctaaaaagc tgatattctg taaagagtta cacggtaaca ttgagatcgc tatgaaatat
    12661 caacaacttg aaaatcttga aagcggttgg aaatggaagt atctggtgaa gaagcatcgc
    12721 gaaggggagt taatcacccg ttacatagaa gccagtgccg cccaggaagc cgtcgatgtg
    12781 ttgctctcgc tcgaaaatga acccgtgctg gtaaatggct ggattgacaa gcatatgaat
    12841 ccggaactgg tcaatcgcat gaagcaaacc attcgggcaa gacgaaagcg ccattttaat
    12901 gcggaacacc agcatacgcg caaaaaatcg atcgatctgg aatttattgt ctggcaacgt
    12961 ctggctggtc ttgcgcagcg tcgcggtaaa acgctttctg aaacgattgt tcagctgatt
    13021 gaagatgcgg aaaacaaaga gaaatacgcg aataaaatgt cttctctgaa gcaggatctg
    13081 caggcattgc tgggtaagga ataactgacg aaagtcagtt caatttacta aaggcaaaaa
    13141 aaccccgcag cagcggggtt tttctaccag acgagaactt aagcttgcgg ctgagttaca
    13201 acgtctttga tacctttaac ttcgatctct acgcgacgat ccggagccag gcagtcgatc
    13261 agtgcagcac gctgtttcac gttgtcacag gtgttgccag taaccgggtt ggattcgccc
    13321 ataccacgtg cggagatctt gtctgccggg atacctttgg agatcaggta atcaacaaca
    13381 gactgagcac ggcgctcgga cagaccctgg ttgtaagcgt cagaaccgat gcggtcggtg
    13441 taacccagaa caactacgga accgtctttc ggatccaggt tgctcagctg gctgtacagc
    13501 tgatccagag cagcctgacc ttccggtttc agggttgctt tgttgaagtt gaacagaacg
    13561 tcagacttca gagtgaagtg cttggtctgt acttccggtg ccggagctgg agccggagca
    13621 actactggag ctgcttcgcc ctgaccgaaa cggtaggaaa cacccaggct cagcatgccg
    13681 ttgtccggac gagtgccgat ggtgtgtgcg tcaccgatgt tgttggtcca ctggtattcc
    13741 agacgggtag cgatttcagg agtgatcgcg tactcaacac cgccagcgaa gaccggagaa
    13801 acgccggtgt cgtggttttt accataaacg ttggatttag tgtctgcacg ccataccata
    13861 ccacccagac gagtgtagat gtccaggtcg tcagtgattg ggtaacccag tttagcggtc
    13921 agttgaacgc cctgagcttt gtatgcaccg ttttcaacgc tgcctttgta cggcatacga
    13981 cctaaccagt cgtaacccat ttcaaagcca acatacgggt taacctggta accaccaaaa
    14041 gcaccagcgc ccagttggtt ttcatgggtc gggccattgt tgttgatgaa accagtgtca
    14101 tggtactggg accagcccag tttagcacca gtgtaccagg tgttatcttt cggagcggcc
    14161 tgcgctacgg tagcgaaacc agccagtgcc actgcaatcg cgatagctgt ctttttcatt
    14221 ttttgcgcct cgttatcatc caaaatacgc catgaatatc tccaacgaga taacacggtt
    14281 aaatccttca ccgggggatc tgctcaatat taactctacc gatatcttcg gcttatgccg
    14341 agcacccctg gcgatgtaaa gtctacaacg tagttgaaaa cttacaagtg tgaactccgt
    14401 caggcatatg aaaaaaaagt cttgtataag gtatgtttaa tcttttttgt cagcgacaat
    14461 ttacagaaga aaatcgcgga aaccgcttca gacaagcctc cgcaaggaaa attagtcacg
    14521 actgaaagca ttggctgggc gacaaaaaaa gttccaggat taatcctaaa tttacttaat
    14581 gatacaaatt agagtgaatt tttagcccgg aaagttgtct cgtggcgtga gaggatgcgc
    14641 ttaccggacg cataataaac cccatagcgt taccttcatt tgccgcatca acaagttcag
    14701 catgctcttc ttcagtcaaa tcatctgcca accaaccgat caccacactg taattgcccg
    14761 tgcgtaaagc gcgaaccatt gactccacag tgtggcaagg ggagagctgg ctaatctgca
    14821 ttactttcgt taagggtagc ccagatgcct gaacccattc ccgactcagt ttttgttgcg
    14881 gtgttaacca gagttgccag cgcgattgct gaccgagttg ctgtaacaat ggcaacagta
    14941 gaagttgcgt catcatgggc tgatcttcgc gatagacaac ttcactgata agcccggctg
    15001 tagtgttttc cgtagagaca cgcgcaattt tacttgctgc ggatgagaac gacgaagaac
    15061 gatgtgcata gcctgaagtg tacataatca atccagcccc tgtgagttac tgtatggatg
    15121 tacagtacat ccagtgacaa caaagatcaa ccctattttc ggaaagagcc tcgcaaattt
    15181 tgtcgttggt gacgggaaaa cataaattaa tcttgcccct taagaataag ttgcctattt
    15241 tcgtagttaa cggatccgtt aatgtgaatc attcttttat gttatgattt taaaaggaat
    15301 tttatgaaaa gcctctccta taagcggatc tataaatcac aagaatacct ggcaacgttg
    15361 ggcacaattg aataccgatc attgtttggc agttacagcc tgaccgttga cgacacggtg
    15421 tttgcgatgg tttctgatgg tgagttgtat cttcgggctt gtgagcaaag tgcacagtac
    15481 tgtgtaaaac atccgcctgt ctggctgaca tataaaaagt gtggccgatc cgttaccctc
    15541 aattactatc gggttgatga aagtctatgg cgaaatcaac tgaagctggt gcgtctgtcg
    15601 aaatattctc tcgatgcagc gctgaaagag aaaagcacgc gcaatacccg ggaaagactg
    15661 aaagatttgc ccaatatgtc ttttcatctg gaagcgattc tcggggaggt ggggattaag
    15721 gatgtacggg cgttacgtat acttggggca aaaatgtgtt ggttgcgact gcggcagcaa
    15781 aacagtctgg tgacagaaaa gattctgttt atgcttgaag gtgccattat cggcattcat
    15841 gaagctgcgc tcccggtggc acgccgccag gagcttgcag aatgggctga ctctcttacg
    15901 ccgaaacagg agtttcctgc ggaacttgag taatctggcg ttgcagacga ccaatctcag
    15961 gcagtaatgc aatcaataat ccaacttgtt gtacgaccag aggttctttg ctgtctgccc
    16021 gtggttcaag ttgctgcatc cgctgtttca ggctagctaa tgcctcattg acgcgttctt
    16081 catcagcagg ttgatgatgt aacgcgtcat caacatagca aactgcgtca tcaagaaacg
    16141 ccagaatttc aggattagtt aactgctccc ggtgagcacc gagggctgag atatagctgg
    16201 taaacgtatg gttaaggcac agcaaccgaa acgcggcttc gcgaatttgc ggggtaacgt
    16261 tcggctcgct ggacatattt gataccaccg acgccagctc agcatcacgg ttgtgtgcat
    16321 cgcggcgggc aatacgatac gccagacggt tatcacgccc ctgatggtat tgctccagta
    16381 tggcatcgag ataccgacag ttggcctctg tggcgcgttc gagcatgcgc ggcagattgc
    16441 gaaactgcca gtcaggccag atgtagctca ctgccgccca cgcaatggca caaccaatca
    16501 gcgtatcgat tacgcgaggt aacgctactt caaaaccttc acccagtaag ttaaaacaca
    16561 gtagcaccaa aagtgtgatg aacatcgttg catgagcgta ttgcacgtta cggaaggcaa
    16621 aaaagagcac gccggtaata accagcagca ccagctgccc ttccagtgat ggcacaaacc
    16681 acagcacagg aatgccaatg gcgataccta ccagcgtacc aataatcctt aacttcaggc
    16741 ggtggcgcgt ggcgttatag tttggctggc agacaaacaa acttgtcagc aagatccaat
    16801 acccgtgatg cattccggtt atttgaatga tggcgtagcc gaagcacaac accagcgaca
    16861 ttcttaccgc atgacggaag agggcggatt ccggcgtgaa gtgacggcta agacgcagcc
    16921 agatatcact caacccgtgc gggctgtcat cagcgagctc attttcgtca ttattatggg
    16981 gtagtgcctg ggcctgttct gattcaattg ttgccagttg ggcatcaatg gcgcgtaaat
    17041 tgttcagcaa aaatcccagt gttttgagta aatcggcggg tgcgccgtta tcgcgcatcc
    17101 gctccagcgc agcatcaata tgcgtaaaag cgcgctcaaa atgcggatca tgttgataag
    17161 gctgacgcaa caaaatacag cgtgacagtt gctggcacgc ctggccctgc atcgacatca
    17221 gccgctgaaa acggaacagc acgtcgctgt ggcgaaaatg ttcacgcaat gtttgatact
    17281 gaatatgaga agagctggca cgctcgtgaa tatcctgtgc gacaaagtaa taatgcagcg
    17341 tgcgacgcgt tccccgttga ccacgatcgc cacgtaagcg ggtcagcagc gagagtttcg
    17401 tctgattcaa tgtcgccatc agcagaccgt tggcgagagc caaatcgtac agcggtgcct
    17461 ggctttcatc ttcaatatca ggatcaaaca tgcgcgactt gagctcaaga taacgcgcca
    17521 gttgttcata gcaacgcgcc aggttgtcct gcagcgggcg gaccgggaac agcagatgac
    17581 caataagtgt caggacgttg taccagacgg caccggccag cagatacatc ggctgctgat
    17641 accagtgctc atacagtgat gttcccaaca tagtgtaaat ggcgatcagc aatgcaccga
    17701 aggcaattgt tgcatagcgt tgacccagac cgccgagcaa aatgaagccg ctggtagaga
    17761 gcgttaagcc aatcgcaaat agccagggcc agggaaacag caattctact gaggccgagg
    17821 cgataaaaaa gcagaacagc gtaatgatga ggttacgcaa acgtcccgcc agtcggtcat
    17881 cgagatcggt cagcgccgct gccaccatcc ccagcgttag cggaatcgtc agttttacat
    17941 cacccagcca ccacggaaac gctgtggttc cacaaagcgc aataaaaata cgcgcgtaat
    18001 acagccaggc gctgttccag gtatagcgtt tgagcaaagg acttagcata aaggccatat
    18061 tagtggatta ttcaaaacga cgacgtgcat tggcttcacg cgcagcttgc gctgtttcta
    18121 ccgataccac gcgacgacca accggccata gtgcaatagc ggcaatttta aagttcgcaa
    18181 tgccgacagg aatgccaatg attgaaatac attgtgcgat gcccgttgca atgtgcatca
    18241 ggcataacca ccagccaaag aaaatcagcc agaaaatatt caataccgta ccgccagtat
    18301 tcagcagtac atttttgcca gccgggttca gttcatcgac atgaatagct tcattgccat
    18361 aaggcaccag agacagttta gtgatctccc agcaggatcg tgtcagcggt aaggtaaaaa
    18421 tcagcacaat actgaccaga gtcgccaaca gccagcccag agtggtggca aatccgccaa
    18481 gcacaaagtt cagaatgttc aaaacggtac gcataaaacc tcgctttact gtggttttca
    18541 gtaatgggcg gcaattgtaa cgtttttttg ggctggagca cgttttctct gacggttaca
    18601 ctgataagaa ataatttcgt gtggatctac agagtcatgg aactgaaagc gacaacgctt
    18661 ggaaaacgtc tggcacagca cccttacgat cgggcggtga tcctcaatgc cgggattaaa
    18721 gtctccggcg atcgccacga ataccttatt cctttcaatc aattactggc gattcactgt
    18781 aagcgcggtc tggtatgggg cgagctggaa tttgtactgc cggacgaaaa agtggtgcgt
    18841 ctgcacggca ccgaatgggg cgagacgcag cgtttttacc atcatcttga tgctcactgg
    18901 cggcggtgga gtggcgagat gagcgaaatt gcgtctggtg ttttacgcca gcaactggat
    18961 ttgattgcca cgcgcactgg ggaaaataaa tggctgacgc gtgagcaaac ctctggcgtg
    19021 cagcaacaaa tccgccaggc tttgtcggcg ttgccgttgc cggttaaccg actggaagaa
    19081 ttcgataact gccgtgaggc gtggcgtaaa tgtcaggcct ggttgaaaga tattgaaagc
    19141 gctcggttgc agcataacca ggcgtatacc gaagccatgc ttaccgagta tgcggatttt
    19201 ttccgccagg tcgagtcttc accgctgaat ccggcgcagg cccgggcagt cgttaatggc
    19261 gagcattctc tgttagtgct ggcaggtgca ggaagcggaa aaacgtcggt gctggtggcc
    19321 cgtgcaggct ggttgctggc gcgtggtgaa gcgtcccctg agcaaatttt attgctggcg
    19381 tttggtcgca aagccgctga agagatggac gagcggattc gcgaacggct acataccgaa
    19441 gacattaccg cacgcacgtt tcatgcgctg gcgctgcata ttattcagca gggcagcaaa
    19501 aaagttccga tagtcagcaa actggaaaat gataccgctg cccgtcatga actctttatt
    19561 gctgagtggc gcaagcaatg cagcgaaaag aaagcgcagg cgaagggctg gcggcaatgg
    19621 ctgacggaag aaatgcagtg gtcagtgcca gaaggtaact tctgggatga tgaaaaatta
    19681 cagcgtcgcc ttgcctctcg cctcgatcgc tgggtaagtc tgatgcggat gcacggtggt
    19741 gcacaggcag aaatgattgc cagtgcaccc gaagagattc gcgatctgtt cagtaaacgt
    19801 atcaagttga tggccccgtt attaaaagcc tggaaaggtg cgctgaaggc agaaaacgct
    19861 gtcgattttt cgggccttat tcatcaggcg attgtgattc tggagaaagg tcgctttatc
    19921 agcccgtgga agcatattct ggttgatgaa tttcaggata tctcgccgca gcgggcagcg
    19981 ttgttagcgg cattacgcaa gcaaaacagt cagacgacgt tgttcgccgt tggtgatgac
    20041 tggcaggcga tttaccgatt cagcggcgcg caaatgtcgc tcaccaccgc tttccatgaa
    20101 aactttggtg aaggcgatcg ctgtgattta gacacgactt accgttttaa cagtcgtatc
    20161 ggtgaggtgg caaaccggtt tattcagcag aacccaggcc agttgaaaaa gccgctaaac
    20221 agcttaacca atggagacga aaaagccgtc acgttattgg atgagagtca acttgacgct
    20281 ttgctggata agctctctgg ttatgccaaa ccggaagagc gcattcttat cctggcgcgt
    20341 taccatcaca tgaggcctgc cagcctggaa aaagcggcaa cacgctggcc gaagttgcaa
    20401 atcgacttta tgaccattca tgccagcaaa gggcaacagg cggattacgt catcatcgtt
    20461 ggcttgcagg aggggagtga tggttttccg gctgcggcgc gggagtcgat tatggaagag
    20521 gcgctactgc caccggttga ggatttcccg gacgctgaag aacggcggtt aatgtacgtg
    20581 gcgctgaccc gggcacgcca tcgggtatgg gcactgttta acaaagagaa tccctctccc
    20641 tttgtggaaa tactgaaaaa tctggatgtg ccggtggcga gaaaaccgta agaaacaggt
    20701 ggcgtttgcc acctgtgcaa tattacttca gacggtccgc gagataacgc tgataatcgg
    20761 ggatcagaat atcgaccgcg tcgttgaaat gcggcgactg gataatgaag tctgccgttg
    20821 ccacgttggt ggcgaccgga atgttccata ccgtcgccag acgcagcaag gctttcacgt
    20881 caggatcgtg cggcacggca tttagtggat cccagaagaa aatcaataca tcaattttcc
    20941 cttctgagat caatgcgcca acctgctggt caccccccat tgggccactc aacatcgcgt
    21001 tgacgttcat gccggttgca cgggaaatca agttaccggt agtgcctgtt gcatacagta
    21061 cgtgttgttc cagtaacggt tgatgccgtt ccacccagct catcagcatt tgtttgcagt
    21121 gatcgtgtgc caccagcgca atatgtttcc gcgcaggtaa agtgcgagtc gtcagttcca
    21181 taatgtacat ccgtagttaa ctttcctaca gattactgta agcacttatc gctgcaagat
    21241 aaagaccgaa aaagcctgcg cacaggcaca aaaatctcag gaagatggtt gtttttccgc
    21301 ccactgcagg aaagtatttc gcgtttgtgg gtcagccagt ttaaaccaat acttcagccg
    21361 ttgttctgtg agcacctgag actgcggtgg aatagcatcc agctcggaaa cacccgaaag
    21421 cagcgtactg tcatctgcca tcatcgtggc aaattgcggt agtgaagcgc gtttcgcgga
    21481 tttgttatag cgttcaacct cgacctcata atcaatcgtt tttgccgttg aggtaatggc
    21541 gagaatatcc agctttaccg gaatcggcgt cgcatcgcca tccaacaatt caaggcgcgg
    21601 cgcggcatca aaatggttag cttcccgctc attctccagg cgaggcaggc gaaaattgac
    21661 ctggttgatg agctgggtat taaaactgac caccagcggt ggggagatat acagccgttc
    21721 ttcactattg gaaagatgaa tcgtcttctc aacgcgaaac actaactgat gcggtccatt
    21781 atccagttca atgctgtccg caccgcgcag cagagagctg gaaaccttct tgccgtcgag
    21841 taccagaaga tcgacatcgg ttgaaagccg cagcgtggtg gcaaaaacgg ataccggcaa
    21901 acataatgca atcaaggtgg tcacgatgcc ggttttcata ggtagctcct gtcaaaagac
    21961 cgcccgaata ataatgtgtc aaaatttttc tttaatcata cttactaaca tatagacata
    22021 tttcccgctt ttgctctcat tcattcgtat tagctgcatg gttggcatgt cggctttgtc
    22081 gtacactttg ttaaacagtc tggaggaaat aatgaaagaa accgatattg caggcatttt
    22141 aacgtccact cacaccattg cgctggtggg ggcaagcgac aaacccgatc gtcccagcta
    22201 tcgcgtgatg aaatatctac tcgaccaggg gtatcacgtc attccggttt cgccaaaagt
    22261 tgccggcaaa acgctgctgg gccagcaggg gtatggcacg ctggcagacg ttccggaaaa
    22321 ggtcgatatg gtcgatgttt tccgtaattc agaagcggcg tggggcgtgg cacaggaagc
    22381 tattgccatt ggcgcgaaaa ccctctggat gcaactgggg gtgattaatg agcaagcggc
    22441 agtactggca cgggatgctg ggttgaatgt ggtgatggat cgctgcccgg ctattgagat
    22501 ccctcgcctg ggcctggcca aataaaaaat ccccggaagg caaaaacctt ccggggattc
    22561 gttcagggat cagttacgca gacgcggggc ctggagttgt ttgcggatgg tctgcgccag
    22621 ctcatccatg gatggctgtt caggatgttc atcctgcaat tcgctactta gctgggcttc
    22681 agccaggtag gtatgaaccg gtaggccgtt atcgtcctcc atcaccacgt gataccacgg
    22741 agcggcgcga agctcgtcat tcaccgccag ctcatcaggc gacggttcag agagcgaata
    22801 aaccgggtcg atatccacga ccactccgag ataacctaac agggaatggc ggacctgctg
    22861 gccgataccg aatttgctgg caatcatagt cacctcccgg gaaatcttta cctttgatat
    22921 aagggtaaaa ttccacattt caagttacat gacgcgacag gcaaaccctt tcagatatag
    22981 cccttccgga taggtagcga tcaccggatg atcggctgcc tgacggaact gctctataaa
    23041 ttgtacatca cggccggcat caattgcggc atccgcgatg attttctgaa ataaatcgct
    23101 ggtcatcaga ccggaacagg agaaagtcag gagaataccg ccttcattca gcaactgaat
    23161 cgccagcatg ttgatatctt tatagccacg acacgcgccc atcaactggc ttttattctc
    23221 aacaaacttc ggcgggtcca tcacgataac gtcaaatatt tcaccgcgat cgcgataagt
    23281 acgcagcaat ttaaagacat catcacggac aaactcagcc ttgctcagat ccagtttgtt
    23341 cagctcaacg ttctgccgtg caatatccag cgcttcctgg gaggtatcaa cgctgacaac
    23401 ctggctgcaa ccgcccatca gtgccgatac ggcgaaacca ccggtatagg agaaacagtt
    23461 cagcacacgt ttattttcaa cgtagcggcg ggtagccagg cggctatcac gctggtccag
    23521 gtagtagccc gttttgtgtc cgtgctgaat atccaccagc agcttcatgc cgtgttcttc
    23581 aatcggcagc agggcaggcg gcaactcgcc ggtgacgagg ccctgggtca gctccatccc
    23641 ttctttttta cgtaccgcaa catcgctgcg atcgtaaatc gcacattccg ggtacagcgt
    23701 ttgcagggca ctaactaatg ccgcgcgctg atattctgcc ccagcactca gcagttgcag
    23761 caccagaaaa ttaccgaaac gatcgatagt aatacccggc aggccatcag attctccggc
    23821 gattaaacga tagctgtcga ggccatcttt ttgcgccagc cagtcacgcc atttttgtgc
    23881 ttgttgcaaa cggcgggaaa aaaaagcaat gtcgatagac tcagacgggt caaacgtcca
    23941 gacgcgcgcc cggatttgcg aagctggtga ataagcgccg cgtgctaacc attttccctg
    24001 atgatcaaca atatcgatgg tttcaccgag gctggcttta ccttccatgc gggcaacggc
    24061 cccggaaaag acccacggat ggcgacgaag taatgatttt tcgcgccctt tggctaacac
    24121 taaacgtaca ctcataattt acttttctgt cgatgcaaag aaatgggcgt cattgtccgg
    24181 agttttccat ggatttgcaa cgcgtccatt aaggataagg agagcagcaa tgtcgaaagt
    24241 ctgcataatt gcctgggttt acgggcgagt tcagggcgta ggatttcgct acaccacaca
    24301 gtacgaagcg aaaagactgg gtttaaccgg gtacgccaaa aatcttgacg atggcagcgt
    24361 ggaagtggtt gcctgcggtg aagaagggca ggtggaaaaa ttaatgctgt ggttaaagag
    24421 tggcggcccg cgttctgcgc gtgtagaacg ggtgctcagc gagccgcatc atccctcggg
    24481 ggaattaacc gattttcgta tccgttaaat acattttacc ggttttggca ggccagcaat
    24541 tttggtggct tgcttagccg gacctttcgg aaacagtcgg tataaatagc ggctgttacc
    24601 tttttcttcg ccaaatttat tcgccatcgc ttttaccagc atacgaatcg ccggagaagt
    24661 attgaattcc agatagaaat cacgcacaaa acgcaccact tcccagtgtt ccggtgacag
    24721 cgaaatccct tcgttctctg caatcaccac tgccagcggc tcactccact ggctgctttc
    24781 tttgagatag ccttcggtat ccgtttctat ctctttacct tcgaagatca gcataattat
    24841 tactacctta atcagactgg cggcagtgta acaaaaaaca aagccccgca taaagcgagg
    24901 ctatgaaagt gttagcgggt gagattaatc gcggctggcg aagcccagaa tgctcagcag
    24961 gctgacgaag atgttgtaca gcgaaacata caggctaacc gtggcacgaa tatagttcgt
    25021 ttcaccgcca tgaacgatgt tgctggtttc aaacaaaata gcgccagagg agatcaaaat
    25081 gaagaccgcg ctgatcgcca gatgcagagc aggcagctgc aggaagatat tagcaaccat
    25141 accaatcagc accaccacaa tacccgccat cagcataccg ccgaggaacg acatatcttt
    25201 gcgggtggtc agcacatatg cagagcagca gaagaacact aacgccgttc cgcccagtgc
    25261 catagcgatt acgtcaccca ttccggcaga cagataggtg ttcagaatag gcccgaggat
    25321 ataacccaga aaaccggtaa aggcgaatgc ggagataatc ccggtcggct tattcgccgt
    25381 tttataggtc aggaacatca aaccatacat acccaccagc gtcagaatca gacccggaga
    25441 tggcagcatc agcaccgtac tggcagttgc ggtaattgcc gaaaaggcca gcgtcaggct
    25501 cagcagaaaa taggtattac gcagcacctt atgggtgcta agcagtgatg tacggtcatg
    25561 tgaagaacta acaatacgat ccattgagtc actctctatg acagatgtaa ttaattaagc
    25621 agcataatga taatgcgtaa gggcacccag aagttttacc catctttacg catttgatct
    25681 ggaacaggtt taacagcgga ttatcaggtc attaagcaaa tataacgccc tgagaatttc
    25741 gacaggcaaa agaaaaaggg gttagcattt agctaacccc ttatcttatt tggcggaagc
    25801 gcagagattc gaactctgga accctttcgg gtcgccggtt ttcaagaccg aagaaaaaca
    25861 aataaaaatc agtgcaatac gtgaaattgc tgaaaagtta atcaaattta acaccaagaa
    25921 aaatcaaggt attaggaagt tgctcatagc tagtatacca agtctttttg ccagaaattt
    25981 ttctggattg tctacttgct aaccacgagc ggcaaatcca gtgttggtgt gatttttgtt
    26041 ttacgatcgt aaatcaacac ctgattttct gttttatgcc cgctgaaaat ttgtttgtcg
    26101 cgactgctgc cttcgtaatc tgagatcccc ttggctttta tgtcgtgaaa attgcacccg
    26161 aacggaacgc caactttttg ctcggctgcg cgtttagcct gattccacca gttattcagc
    26221 gttttagcta tgactttccc gcctttggtt gtattgatca catattcgca tgtacaggaa
    26281 gatacatttc gggctaactg gatcgctgta cgtaatcgcg gtgaccattc cttgatttgt
    26341 ttggtgccgg ttttgttttg ctcaatgtag atccctttat ccataatatc ctgccatttc
    26401 aactcaagca catcaccgag tcttgccgca cagagatagg aaatctccat tgcaatgcgt
    26461 aactgtggaa ttgcttccgc atatatcgcc gcatactgtt catcggtgat gtaaacagtg
    26521 cgggctttaa gagagaattt tctgactcct ttgcatgggt tattcttcac atacccacgc
    26581 tcatatcccc acccgtatac tcgactcagg cttgccagtt catgatttgc ctgggtcttg
    26641 ctctcaagcc ctcgtttatc catgaaaatt cttacctgct cagttttgac attatcagca
    26701 agcacttttc cgaataccat cagcaacgcc ttctgatgtt gtcgataatc tttttgggtt
    26761 cggggggcca gttctgtaaa tgcaggggag tccataaaca tgtgccataa tttagctacg
    26821 gtcattatgt tgtggagttt tgctttttcc agttcataat tttgccagac tttagctacg
    26881 ctggttttcc gcactcttcc tagccctata cttcttgtac ttccttcggg tttccacacg
    26941 taactgtaac cattcgatct aacccgcggt ggcagtacat tatctttttt attttttctt
    27001 ggtcttccca ttgttcagcg cctcaaaatc gggttcagca gaaaccagtt cagatgtttt
    27061 tggcatcgtt gtcagaccgt gtggaatatc cctgcggaga actatgggtt cgtttttagg
    27121 gccgattaca aatgggatgc cgtgcagtct taactggtgt tgctgttttg tgtatcgctc
    27181 gtatttcgtg atctctttaa tctctgctgg agatagagtt aattcgtaca tgtggtcacg
    27241 tgcctttaca gcatgaccgc cgccactata gctggtgggc ggcgatatgg gttgaacatc
    27301 aaaaatcagc ctgactcggg agcagttttt gccagatggc tgaaacgtat ttcgcctggt
    27361 aacgtgcatc gttcagagcg ttatggcgtt caccttcgaa tggaatagtc gttcttgcgt
    27421 cgaaatccat caccagtccc agagcaacca tcgttcttac atcgcgatca ttggtgtaac
    27481 gccacgggca ggggatcccc tgccgttcat atgaacggcg taaaatcacg ttgtcgaaag
    27541 ttgcaccgtt accccagacc tgaacaaaaa attcaccgga gttttcgtcg ataaattccc
    27601 gtaattgcag cagtgcatca tctaacggga tttcatcggt aagaatggcg gattgcgctt
    27661 cgcgtgactg tttcagccac cacttaatgg tgtcccgatc aatgactccg ccagcagttt
    27721 ccagatcgat agttttgctg aattctggcc ccatctctcc ggtttgcgga tcgaaaaata
    27781 ttgcacctat tgagataatc ggggcatcag gattttttcc catggtttca aggtcgatca
    27841 tcagatgaat ccccgctctg ctggtggatg tgagattatg atgaccgttc gccttaatta
    27901 agggatctga cgcctcgcca gtttcactat cgctggcatg atgctgattg ccgccagtgt
    27961 tctccttgtg ctgatgcgca gtgccttcca tttcctccgg atcattttcc tgaacttcag
    28021 gctgattctc ttcatcgaat atttcctggt atgttgcgtc acttatcacc gcaccacaat
    28081 cagggcagct accgccgccg gtctgaccgc aggcggtgca ggctttttcc ggctcctgtt
    28141 tcgtttctgg ctcgttttgt tgcgcatttg ggctgttttg ttccgttttc tggtcgttct
    28201 gttccgattc ttgctggttc tggttcacag aatcgcaggt ttcaatcccc ttcacccatt
    28261 tcggatcatt cggatcgcta atcccttcaa caaattcacc acgtgatgca gcaagcaatt
    28321 tatcggcgtc aggctggctg atattggctg cctgcataat tttgtttact tcgtcagcgg
    28381 taacttttac cggctccggt tgtacggaat cttcagcggt atccac
//
LOCUS       DAZKHF020000032        28332 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2201S31241
            SAMN43267800-rid25106413.denovo.032, whole genome shotgun sequence.
ACCESSION   DAZKHF020000032 DAZKHF020000000
VERSION     DAZKHF020000032.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267800
            Sequence Read Archive: SRR30307263
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 28332)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 28332)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 28332)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350581.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350581.2
            Genome Coverage       :: 151x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:21:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,652
            CDSs (total)                      :: 5,557
            Genes (coding)                    :: 5,320
            CDSs (with protein)               :: 5,320
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 237
            CDSs (without protein)            :: 237
            Pseudo Genes (ambiguous residues) :: 0 of 237
            Pseudo Genes (frameshifted)       :: 102 of 237
            Pseudo Genes (incomplete)         :: 130 of 237
            Pseudo Genes (internal stop)      :: 52 of 237
            Pseudo Genes (multiple problems)  :: 41 of 237
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28332
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267800-rid25106413.denovo.032"
                     /serotype="O 26:H11"
                     /isolate="2201S31241"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-01"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            complement(84..1187)
                     /gene="gldA"
                     /locus_tag="ACHOSC_RS05725"
                     /old_locus_tag="pgaptmp_001145"
     CDS             complement(84..1187)
                     /gene="gldA"
                     /locus_tag="ACHOSC_RS05725"
                     /old_locus_tag="pgaptmp_001145"
                     /EC_number="1.1.1.6"
                     /EC_number="1.1.1.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418380.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional L-1,2-propanediol
                     dehydrogenase/glycerol dehydrogenase"
                     /protein_id="HGU4547124.1"
                     /translation="MDRIIQSPGKYIQGADVINRLGEYLKPLAERWLVVGDKFVLGFA
                     QSTVEKSFKDAGLVVEIAPFGGECSQNEIDRLRGIAETAQCGAILGIGGGKTLDTAKA
                     LAHFMGVPVAIAPTIASTDAPCSALSVIYTDEGEFDRYLLLPNNPNMVIVDTKIVAGA
                     PARLLAAGIGDALATWFEARACSRSGATTMAGGKCTQAALALAELCYNTLLEEGEKAM
                     LAAEQHVVTPALERVIEANTYLSGVGFESGGLAAAHAVHNGLTAIPDAHHYYHGEKVA
                     FGTLTQLVLENAPVEEIETVAALSHAVGLPITLAQLDIKEDVPAKMRIVAEAACAEGE
                     TIHNMPGGATPDQVYAALLVADQYGQRFLQEWE"
     gene            complement(1198..1860)
                     /gene="fsa"
                     /locus_tag="ACHOSC_RS05730"
                     /old_locus_tag="pgaptmp_001146"
     CDS             complement(1198..1860)
                     /gene="fsa"
                     /locus_tag="ACHOSC_RS05730"
                     /old_locus_tag="pgaptmp_001146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016832 - aldehyde-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-6-phosphate aldolase"
                     /protein_id="HGU4547125.1"
                     /translation="MELYLDTANVAEVERLARIFPIAGVTTNPSIIAASKESIWEVLP
                     RLQKAIGDEGILFAQTMSRDAQGMVEEAKRLRDAIPGIVVKIPVTSEGLAAIKMLKKE
                     GITTLGTAVYSAAQGLLAALAGAKYVAPYVNRVDAQGGDGIRTVQELQALLEMHAPES
                     MVLAASFKTPRQALDCLLAGCESITLPLDVAQQMLNTPAVESAIEKFEHDWNAAFGTT
                     HL"
     gene            complement(1872..4373)
                     /gene="ptsP"
                     /locus_tag="ACHOSC_RS05735"
                     /old_locus_tag="pgaptmp_001147"
                     /pseudo
     CDS             complement(1872..4373)
                     /gene="ptsP"
                     /locus_tag="ACHOSC_RS05735"
                     /old_locus_tag="pgaptmp_001147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312903.2"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0008965 - phosphoenolpyruvate-protein
                     phosphotransferase activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate--protein phosphotransferase"
     gene            4682..5761
                     /gene="frwC"
                     /locus_tag="ACHOSC_RS05740"
                     /old_locus_tag="pgaptmp_001148"
     CDS             4682..5761
                     /gene="frwC"
                     /locus_tag="ACHOSC_RS05740"
                     /old_locus_tag="pgaptmp_001148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit EIIC"
                     /protein_id="HGU4547126.1"
                     /translation="MNELVQILKNTRQHLMTGVSHMIPFVVSGGILLAVSVMLYGKGA
                     VPDAVADPNLKKLFDIGVAGLTLMVPFLAAYIGYSIAERSALAPCAIGAWVGNSFGAG
                     FFGALIAGIIGGIVVHYLKKIPVHKVLRSVMPIFIIPIVGTLITAGIMMWGLGEPVGA
                     LTNSLTQWLQGMQQGSIVMLAVIMGLMLAFDMGGPVNKVAYAFMLICVAQGVYTVVAI
                     AAVGICIPPLGMGLATLIGRKNFSAEERETGKAALVMGCVGVTEGAIPFAAADPLRVI
                     PSIMVGSVCGAVTAALVGAQCYAGWGGLIVLPVVEGKLGYIAAVAVGAVVTAVCVNVL
                     KSLARKNGSSTDEKEDDLDLDFEIN"
     gene            5776..6096
                     /gene="frwB"
                     /locus_tag="ACHOSC_RS05745"
                     /old_locus_tag="pgaptmp_001149"
     CDS             5776..6096
                     /gene="frwB"
                     /locus_tag="ACHOSC_RS05745"
                     /old_locus_tag="pgaptmp_001149"
                     /EC_number="2.7.1.202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022877 -
                     protein-N(PI)-phosphohistidine-fructose phosphotransferase
                     system transporter activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose-like transporter subunit IIB"
                     /protein_id="HGU4547127.1"
                     /translation="MTKIIAVTACPSGVAHTYMAAEALESAAKAKGWEVKVETQGSIG
                     LENELTAEDVASADMVILTKDIGIKFEERFAGKTIVRVNISDAVKRADAIMSKIEAHL
                     AQTA"
     gene            6147..8440
                     /gene="pflD"
                     /locus_tag="ACHOSC_RS05750"
                     /old_locus_tag="pgaptmp_001150"
                     /pseudo
     CDS             6147..8440
                     /gene="pflD"
                     /locus_tag="ACHOSC_RS05750"
                     /old_locus_tag="pgaptmp_001150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709751.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="formate C-acetyltransferase"
     gene            8406..9284
                     /gene="pflC"
                     /locus_tag="ACHOSC_RS05755"
                     /old_locus_tag="pgaptmp_001151"
     CDS             8406..9284
                     /gene="pflC"
                     /locus_tag="ACHOSC_RS05755"
                     /old_locus_tag="pgaptmp_001151"
                     /EC_number="1.97.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312908.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="[formate-C-acetyltransferase]-activating enzyme"
                     /protein_id="HGU4547128.1"
                     /translation="MTSSAGQRISCNVVETRRNDVARIFNIQRYSLNDGEGIRTVVFF
                     KGCPHLCPWCANPESISGKIQTVRREAKCLHCAKCLRDADECPSGAFERIGRDISLDA
                     LEREVMKDDIFFRTSGGGVTLSGGEVLMQAEFATRFLQRLRLWGVSCAIETAGDVPAS
                     KLLPLAKLCDEVLFDLKIMDATQARDVVKMNLPRVLENLRLLVSEGVNVIPRLPLIPG
                     FTLSRENMQQALDVLIPLKIRQIHLLPFHQYGEPKYRLLGKTWSMKEVPAPSSADVTT
                     MREMAERAGFQVTVGG"
     gene            9286..9627
                     /gene="frwD"
                     /locus_tag="ACHOSC_RS05760"
                     /old_locus_tag="pgaptmp_001152"
     CDS             9286..9627
                     /gene="frwD"
                     /locus_tag="ACHOSC_RS05760"
                     /old_locus_tag="pgaptmp_001152"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose-like transporter subunit IIB"
                     /protein_id="HGU4547129.1"
                     /translation="MAYLVAVTACVSGVAHTYMAAERLEKLCQLEKWGVSIETQGALG
                     TENRLADEDIRRADVALLITDIELAGAERFEHCRYVQCSIYAFLREPQRVMSAVRKVL
                     SAPQQTHLILE"
     gene            complement(9614..10465)
                     /gene="yijO"
                     /locus_tag="ACHOSC_RS05765"
                     /old_locus_tag="pgaptmp_001153"
     CDS             complement(9614..10465)
                     /gene="yijO"
                     /locus_tag="ACHOSC_RS05765"
                     /old_locus_tag="pgaptmp_001153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HGU4547130.1"
                     /translation="MYHDVSYLLSRLINGPLSLRQIYFASSNGPVPDLAYQVDFPRLE
                     IVLEGEFVDTGAGATLVPGDVLYVPAGGWNFPQWQAPATTFSVLFGKQQLGFSVVQWD
                     GKQYQNLAKQHVARRGPRIGSFLLQTLNEMQMQPQEQQTARLIVASLLSHCRDLLGSQ
                     IQTASRSQALFEAIRDYIDERYASALTRESVAQAFYISPNYLSHLFQKTGAIGFNEYL
                     NHTRLEHAKTLLKGYDLKVKEVAHACGFVDSNYFCRLFRKNTERSPSEYRRQYHSQLT
                     EKPTTPE"
     gene            complement(10614..12347)
                     /gene="eptC"
                     /locus_tag="ACHOSC_RS05770"
                     /old_locus_tag="pgaptmp_001154"
     CDS             complement(10614..12347)
                     /gene="eptC"
                     /locus_tag="ACHOSC_RS05770"
                     /old_locus_tag="pgaptmp_001154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoethanolamine transferase CptA"
                     /protein_id="HGU4547131.1"
                     /translation="MHSTEVQAKPLFSWKALGWALLYFWFFSTLLQAIIYISGYSGTN
                     GIRDSLLFSSLWLIPVFLFPKRIKIIAAVIGVVLWAASLAALCYYVIYGQEFSQSVLF
                     VMFETNTNEASEYLSQYFSLKIVLIALAYTAVAVLLWTRLHPVYIPKPWRYVVSFALL
                     YGLILHPIAMNTFIKNKPFEKTLDNLASRMEPAAPWQFLTGYYQYRQQLNSLTKLLNE
                     NNALPPLANFKDESGNEPRTLVLVIGESTQRGRMSLYGYPRETTPELDALHKTDPNLT
                     VFNNVVTSRPYTIEILQQALTFANEKNPDLYLTQPSLMNMMKQAGYKTFWITNQQTMT
                     ARNTMLTVFSKQTDKQFYMNQQRTQSAREYDTNVLKPFQEVLKDPAPKKLIIVHLLGT
                     HIKYKYRYPENQGKFDGNTDHVPPGLNAEELESYNDYDNANLYNDHVVASLIKDFKAA
                     NPNGFLVYFSDHGEEVYDTPPHKTQGRNEDNPTRHMYTIPFLLWTSEKWQATHPRDFS
                     QDVDRKYSLAELIHTWSDLAGLSYDGYDPTRSVVNPQFKETTRWIGNPYKKNALIDYD
                     TLPYGDQVGNQ"
     gene            complement(12530..15181)
                     /gene="ppc"
                     /locus_tag="ACHOSC_RS05775"
                     /old_locus_tag="pgaptmp_001155"
     CDS             complement(12530..15181)
                     /gene="ppc"
                     /locus_tag="ACHOSC_RS05775"
                     /old_locus_tag="pgaptmp_001155"
                     /EC_number="4.1.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004203687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008964 - phosphoenolpyruvate carboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA];
                     GO_process: GO:0015977 - carbon fixation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate carboxylase"
                     /protein_id="HGU4547132.1"
                     /translation="MNEQYSALRSNVSMLGKVLGETIKDALGEHILERVETIRKLSKS
                     SRAGNDANRQELLTTLQNLSNDELLPVARAFSQFLNLANTAEQYHSISPKGEAASNPE
                     VIARTLRKLKNQPELSEDTIKKAVESLSLELVLTAHPTEITRRTLIHKMVEVNACLKQ
                     LDNKDIADYEHNQLMRRLRQLIAQSWHTDEIRKLRPSPVDEAKWGFAVVENSLWQGVP
                     NYLRELNEQLEENLGYKLPVEFVPVRFTSWMGGDRDGNPNVTADITRHVLLLSRWKAT
                     DLFLKDIQVLVSELSMVEATPELLALVGEEGAAEPYRYLMKNLRSRLMATQAWLEARL
                     KGEELPKPEGLLTQNEELWEPLYACYQSLQACGMGIIANGDLLDTLRRVKCFGVPLVR
                     IDIRQESTRHTEALGELTRYLGIGDYESWSEADKQAFLIRELNSKRPLLPRNWQPSAE
                     TREVLDTCQVIAEAPQGSIAAYVISMAKTPSDVLAVHLLLKEAGIGFAMPVAPLFETL
                     DDLNNANDVMTQLLNIDWYRGLIQGKQMVMIGYSDSAKDAGVMAASWAQYQAQDALIK
                     TCEKAGIELTLFHGRGGSIGRGGAPAHAALLSQPPGSLKGGLRVTEQGEMIRFKYGLP
                     EITVSSLSLYTGAILEANLLPPPEPKESWRRIMDELSVISCDLYRGYVRENKDFVPYF
                     RSATPEQELGKLPLGSRPAKRRPTGGVESLRAIPWIFAWTQNRLMLPAWLGAGTALQK
                     VVEDGKQSELEAMCRDWPFFSTRLGMLEMVFAKADLWLAEYYDQRLVDKALWPLGKEL
                     RNLQEEDIKVVLAIANDSHLMADLPWIAESIQLRNIYTDPLNVLQAELLHRSRQAEKE
                     GQEPDPRVEQALMVTIAGIAAGMRNTG"
     gene            complement(15533..16684)
                     /gene="argE"
                     /locus_tag="ACHOSC_RS05780"
                     /old_locus_tag="pgaptmp_001156"
     CDS             complement(15533..16684)
                     /gene="argE"
                     /locus_tag="ACHOSC_RS05780"
                     /old_locus_tag="pgaptmp_001156"
                     /EC_number="3.5.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019213 - deacetylase activity [Evidence
                     IEA];
                     GO_process: GO:0006526 - arginine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetylornithine deacetylase"
                     /protein_id="HGU4547133.1"
                     /translation="MKNKLPPFIEIYRALIATPSISATEEALDQSNADLITLLADWFK
                     DLGFNVEVQPVPGTRNKFNMLASIGQGAGGLLLAGHTDTVPFDDGRWTRDPFTLTEHD
                     GKLYGLGTADMKGFFAFILDALRDVDVTKLKKPLYILATADEETSMAGARYFAETTAL
                     RPDCAIIGEPTSLQPVRAHKGHISNAIRIQGQSGHSSDPARGVNAIELMHDAIGHILQ
                     LRDNLKERYHYEAFTVPYPTLNLGHIHGGDASNRICACCELHMDIRPLPGMTLNELNG
                     LLNDALAPVSERWPGRLTVDELHPPIPGYECPPNHQLVEVVEKLLGAKTEVVNYCTEA
                     PFIQTLCPTLVLGPGSINQAHQPDEYLETRFIKPTRELITQVIHHFCWH"
     gene            16838..17842
                     /gene="argC"
                     /locus_tag="ACHOSC_RS05785"
                     /old_locus_tag="pgaptmp_001157"
     CDS             16838..17842
                     /gene="argC"
                     /locus_tag="ACHOSC_RS05785"
                     /old_locus_tag="pgaptmp_001157"
                     /EC_number="1.2.1.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005122770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003942 -
                     N-acetyl-gamma-glutamyl-phosphate reductase activity
                     [Evidence IEA];
                     GO_function: GO:0070401 - NADP+ binding [Evidence IEA];
                     GO_process: GO:0006526 - arginine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0006592 - ornithine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyl-gamma-glutamyl-phosphate reductase"
                     /protein_id="HGU4547134.1"
                     /translation="MLNTLIVGASGYAGAELVTYVNRHPHMNITALTVSAQSNDAGKL
                     ISDLHPQLKGIVDLPLQPMSDISEFSPGVDVVFLATAHEVSHDLAPQFLEAGCVVFDL
                     SGAFRVNDATFYEKYYGFTHQYPELLEQAAYGLAEWCGNKLKEANLIAVPGCYPTAAQ
                     LALKPLIDADLLDLNQWPVINATSGVSGAGRKAAISNSFCEVSLQPYGVFTHRHQPEI
                     ATHLGADVIFTPHLGNFPRGILETITCRLKPGVTQAQVAQALQQAYAHKPLVRLYDKG
                     VPALKNVVGLPCCDIGFAVQGEHLIIVATEDNLLKGAAAQAVQCANIRFGYAETQSLI
                     "
     gene            17850..18626
                     /gene="argB"
                     /locus_tag="ACHOSC_RS05790"
                     /old_locus_tag="pgaptmp_001158"
     CDS             17850..18626
                     /gene="argB"
                     /locus_tag="ACHOSC_RS05790"
                     /old_locus_tag="pgaptmp_001158"
                     /EC_number="2.7.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418394.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003991 - acetylglutamate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006592 - ornithine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetylglutamate kinase"
                     /protein_id="HGU4547135.1"
                     /translation="MMNPLIIKLGGVLLDSEEALERLFSALVNYRESHQRPLVIVHGG
                     GCVVDELMKGLNLPVKKKNGLRVTPADQIDIITGALAGTANKTLLAWAKKHQIAAVGL
                     FLGDGDSVKVTQLDEELGHVGLAQPGSPKLINTLLENGYLPVVSSIGVTDEGQLMNVN
                     ADQAATALAATLGADLILLSDVSGILDGKGQRIAEMTAAKAEQLIEQGIITDGMIVKV
                     NAALDAARTLGRPVDIASWRHAEQLPALFNGMPMGTRILA"
     gene            18687..20060
                     /gene="argH"
                     /locus_tag="ACHOSC_RS05795"
                     /old_locus_tag="pgaptmp_001159"
     CDS             18687..20060
                     /gene="argH"
                     /locus_tag="ACHOSC_RS05795"
                     /old_locus_tag="pgaptmp_001159"
                     /EC_number="4.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004056 - argininosuccinate lyase activity
                     [Evidence IEA];
                     GO_process: GO:0042450 - arginine biosynthetic process via
                     ornithine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate lyase"
                     /protein_id="HGU4547136.1"
                     /translation="MALWGGRFTQAADQRFKQFNDSLRFDYRLAEQDIVGSVAWSKAL
                     VTVGVLTAEEQAQLEEALNVLLEDVRARPQQILESDAEDIHSWVEGKLIDKVGQLGKK
                     LHTGRSRNDQVATDLKLWCKDTVGELLTANRQLQSALVETAQNNQDAVMPGYTHLQRA
                     QPVTFAHWCLAYVEMLARDESRLQDALKRLDVSPLGCGALAGTAYEIDREQLAGWLGF
                     ASATRNSLDSVSDRDHVLELLSAAAIGMVHLSRFAEDLIFFNTGEAGFVELSDRVTSG
                     SSLMPQKKNPDALELIRGKCGRVQGALTGMMMTLKGLPLAYNKDMQEDKEGLFDALDT
                     WLDCLHMAALVLDGIQVKRPRCQEAAQQGYANATELADYLVAKGVPFREAHHIVGEAV
                     VEAIRQGKPLEDLPLDELQKFSPVIDEDVYPILSLQSCLDKRAAKGGVSPQQVAQAIA
                     FAQARLG"
     gene            20327..21244
                     /gene="oxyR"
                     /locus_tag="ACHOSC_RS05800"
                     /old_locus_tag="pgaptmp_001160"
     CDS             20327..21244
                     /gene="oxyR"
                     /locus_tag="ACHOSC_RS05800"
                     /old_locus_tag="pgaptmp_001160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016831323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator OxyR"
                     /protein_id="HGU4547137.1"
                     /translation="MNIRDLEYLVALAEHRHFRRAADSCHVSQPTLSGQIRKLEDELG
                     VMLLERTSRKVLFTQAGMLLVDQARTVLREVKVLKEMASQQGETMSGPLHIGLIPTVG
                     PYLLPHIIPMLHQTFPKLEMYLHEAQTHQLLAQLDSGKLDCVILALVKESEAFIEVPL
                     FDEPMLLAIYEDHPWANRECVPMADLAGEKLLMLEDGHCLRDQAMGFCFEAGADEDTH
                     FRATSLETLRNMVAAGSGITLLPALAVPPERKRDGVVYLPCIKPEPRRTIGLVYRPGS
                     PLRSRYEQLAEAIRARMDGHFDKVLKQAV"
     gene            complement(21227..22627)
                     /gene="sthA"
                     /locus_tag="ACHOSC_RS05805"
                     /old_locus_tag="pgaptmp_001161"
     CDS             complement(21227..22627)
                     /gene="sthA"
                     /locus_tag="ACHOSC_RS05805"
                     /old_locus_tag="pgaptmp_001161"
                     /EC_number="1.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418397.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003957 - NAD(P)+ transhydrogenase
                     (Si-specific) activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0045454 - cell redox homeostasis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Si-specific NAD(P)(+) transhydrogenase"
                     /protein_id="HGU4547138.1"
                     /translation="MPHSYDYDAIVIGSGPGGEGAAMGLVKQGARVAVIERYQNVGGG
                     CTHWGTIPSKALRHAVSRIIEFNQNPLYSDHSRLLRSSFADILNHADNVINQQTRMRQ
                     GFYERNHCEILQGNARFVDEHTLALDCPDGSVETLTAEKFVIACGSRPYHPTDVDFTH
                     PRIYDSDSILSMHHEPRHVLIYGAGVIGCEYASIFRGMDVKVDLINTRDRLLAFLDQE
                     MSDSLSYHFWNSGVVIRHNEEYEKIEGCDDGVIMHLKSGKKLKADCLLYANGRTGNTD
                     SLALQNIGLETDSRGQLKVNSMYQTAQPHVYAVGDVIGYPSLASAAYDQGRIAAQALV
                     KGEATAHLIEDIPTGIYTIPEISSVGKTEQQLTAMKVPYEVGRAQFKHLARAQIVGMN
                     VGTLKILFHRETKEILGIHCFGERAAEIIHIGQAIMEQKGGGNTIEYFVNTTFNYPTM
                     AEAYRVAALNGLNRLF"
     gene            22958..23608
                     /gene="fabR"
                     /locus_tag="ACHOSC_RS05810"
                     /old_locus_tag="pgaptmp_001162"
     CDS             22958..23608
                     /gene="fabR"
                     /locus_tag="ACHOSC_RS05810"
                     /old_locus_tag="pgaptmp_001162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002460670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional repressor FabR"
                     /protein_id="HGU4547139.1"
                     /translation="MMGVRAQQKEKTRRSLVEAAFSQLSAERSFTSLSLREVAREAGI
                     APTSFYRHFRDVDELGLTMVDESGLMLRQLMRQARQRIAKGGSVIRTSVSTFMEFIGN
                     NPNAFRLLLRERSGTSAAFRAAVAREIQHFIAELADYLELENHMPRAFTEAQAEAMVT
                     IVFSAGAEALDVGVEQRRQLEERLVLQLRMISKGAYYWYRREQEKTAIIPGNVKDE"
     gene            23608..23967
                     /gene="yijD"
                     /locus_tag="ACHOSC_RS05815"
                     /old_locus_tag="pgaptmp_001163"
     CDS             23608..23967
                     /gene="yijD"
                     /locus_tag="ACHOSC_RS05815"
                     /old_locus_tag="pgaptmp_001163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YijD family membrane protein"
                     /protein_id="HGU4547140.1"
                     /translation="MKQANQDRGTLLLALVAGLSINGTFAALFSSIVPFSVFPIISLV
                     LTVYCLHQRYLNRTMPVGLPGLAAACFILGVLLYSTVVRAEYPDIGSNFFPAVLSVIM
                     VFWIGAKMRNRKQEVAE"
     gene            complement(24007..25107)
                     /gene="trmA"
                     /locus_tag="ACHOSC_RS05820"
                     /old_locus_tag="pgaptmp_001164"
     CDS             complement(24007..25107)
                     /gene="trmA"
                     /locus_tag="ACHOSC_RS05820"
                     /old_locus_tag="pgaptmp_001164"
                     /EC_number="2.1.1.35"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030697 - tRNA
                     (uracil(54)-C5)-methyltransferase activity, S-adenosyl
                     methionine-dependent [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (uridine(54)-C5)-methyltransferase TrmA"
                     /protein_id="HGU4547141.1"
                     /translation="MTPEHLPTEQYEAQLAEKVVRLQSMMAPFSDLVPEVFRSPVSHY
                     RMRAEFRIWHDGDDLYHIIFDQQTKSRIRVDSFPAASELINQLMTAMIAGVRNNPVLR
                     HKLFQIDYLTTLSNQAVVSLLYHKKLDDEWRQEAEALRDALRAQNLNVHLIGRATKTK
                     IELDQDYIDERLPVAGKEMIYRQVENSFTQPNAAMNIQMLEWALDVTKGSKGDLLELY
                     CGNGNFSLALARNFDRVLATEIAKPSVAAAQYNIAANHIDNVQIIRMAAEEFTQAMNG
                     VREFNRLQGIDLKSYQCETIFVDPPRSGLDSETEKMVQAYPCILYISCNPETLCKNLE
                     TLSQTHKVERLALFDQFPYTHHMECGVLLTAK"
     regulatory      25221..25411
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00174"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="cobalamin riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="adenosylcobalamin"
                     /db_xref="RFAM:RF00174"
     gene            25476..27320
                     /gene="btuB"
                     /locus_tag="ACHOSC_RS05825"
                     /old_locus_tag="pgaptmp_001165"
     CDS             25476..27320
                     /gene="btuB"
                     /locus_tag="ACHOSC_RS05825"
                     /old_locus_tag="pgaptmp_001165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015420 - ABC-type vitamin B12 transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015889 - cobalamin transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent vitamin B12 receptor BtuB"
                     /protein_id="HGU4547142.1"
                     /translation="MIKKASLLTACSVTAFSAWAQDTSPDTLVVTANRFEQPRSTVLA
                     PTTVVTRQDIDRWQSTSVNDVLRRLPGVDITQNGGSGQLSSIFIRGTNASHVLVLIDG
                     VRLNLAGVSGSADLSQFPIALVQRVEYIRGPRSAVYGSDAIGGVVNIITTRDEPGTEI
                     SAGWGSNSYQNYDVSTQQQLGDKTRVTLLGDYAHTHGYDVVAYGNTGTQAQTDNDGFL
                     SKTLYGALEHNFTDAWSGFVRGYGYDNRTNYDAYYSPGSPLLDTRKLYSQSWDAGLRY
                     NGELIKSQLITSYSHSKDYNYDPHYGRYDSSATLDEMKQYTVQWANNVIVGHGSIGAG
                     VDWQKQTTTPGTGYVENGYDQRNTGIYLTGLQQVGDFTFEGAARSDDNSQFGRHGTWQ
                     TSAGWEFIEGYRFIASYGTSYKAPNLGQLYGFYGNPNLDPEKSKQWEGAFEGLTAGVN
                     WRISGYRNDVSDLIDYDDHTLKYYNEGKARIKGVEATANFDTGPLTHTVSYDYVDARN
                     AITDTPLLRRAKQQVKYQLDWQLYDFDWGITYQYLGTRYDKDYSSYPYQTVKMGGVSL
                     WDLAVAYPVTSHLTVRGKIANLFDKDYETVYGYQTAGREYTLSGSYTF"
     gene            27265..28122
                     /gene="murI"
                     /locus_tag="ACHOSC_RS05830"
                     /old_locus_tag="pgaptmp_001166"
     CDS             27265..28122
                     /gene="murI"
                     /locus_tag="ACHOSC_RS05830"
                     /old_locus_tag="pgaptmp_001166"
                     /EC_number="5.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312925.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008881 - glutamate racemase activity
                     [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate racemase"
                     /protein_id="HGU4547143.1"
                     /translation="MATKLQDGNTPCLAATPSEPRPTVLVFDSGVGGLSVYDEIRHLL
                     PDLHYIYAFDNVAFPYGEKSEEFIVERVVAIVTAVQERYPLALAVVACNTASTVSLPA
                     LREKFDFPVVGVVPAIKPAARLTANGIVGLLATRGTVKRSYTHELIARFANECQIEML
                     GSAEMVELAEAKLHGEDVSLDALKRILRPWLRMKEPPDTVVLGCTHFPLLQEELLQVL
                     PEGTRLVDSGAAIARRTAWLLEHEAPDAKSADANIAFCMAMTPEAEQLLPVLQRYGFE
                     TLEKLAVLG"
BASE COUNT         6475 a         7123 c         7813 g         6921 t
ORIGIN      
        1 gggataatac aaggcctgat aagcgtagcg catcaggcta ttttgcgttc aaactcccgg
       61 acaagccggg agtttggagt aggttattcc cactcttgca ggaaacgctg accgtactgg
      121 tcggctacca gcagagcggc gtaaacctga tctggcgtcg cgccgccagg catgttgtgg
      181 atggtttcac cttctgcaca tgccgcttct gccacaattc gcattttcgc cgggacatct
      241 tctttaatat ccagttgagc gagagttatt ggcaaaccta ccgcatggct aagtgcagct
      301 acggtttcga tttcctcaac cggcgcgttt tccagtacca gctgcgtcag cgtaccgaat
      361 gccacttttt caccgtgata atagtgatgc gcgtccggga tagcggtcag gccgttatgc
      421 accgcatgag ccgccgccag tccgccgctt tcaaaaccaa cgccgcttaa ataggtgttc
      481 gcttcaatca cgcgttccag cgcaggggtt actacatgct gttcggcggc aagcatcgct
      541 ttttcgcctt cttccagcag agtgttgtag cacagttctg ccagtgccag cgccgcctgg
      601 gtgcacttgc cgcccgccat agtggtagcg ccgctacggg aacaggcgcg tgcttcaaac
      661 caggttgcca gcgcatcgcc gatacccgcc gctaacagac gtgcaggagc gcccgcgacg
      721 attttggtgt cgacaatgac catattcggg ttatttggca acagtagata gcggtcaaac
      781 tcaccctcat cggtgtagat aacagacaat gcgctgcacg gtgcatcggt agaggcgata
      841 gtcggtgcga tcgctaccgg aacacccatg aaatgtgcca gtgctttggc agtatcgagg
      901 gtttttccgc caccgatacc gagaattgcg ccacactgcg cagtctccgc gatgccacgc
      961 agacgatcga tctcattttg cgaacattca ccgccaaacg gcgcaatttc tactaccagt
     1021 ccagcatctt taaagctttt ctcgacagtg gattgagcaa aacctaaaac aaatttgtca
     1081 cccaccacta accagcgttc tgccagcggc ttcaggtatt cgcccagacg attaatcaca
     1141 tcagcgccct ggatgtattt acccggtgat tgaataatgc ggtccataat tgctccttta
     1201 gagatgagta gtgccaaatg cggcattcca gtcgtgttcg aacttctcta tagctgactc
     1261 taccgcaggg gtgttgagca tttgttgcgc tacatctaaa ggcagggtga tggactcaca
     1321 tcctgccagc aaacaatcca gtgcctgacg cggcgtttta aagctggctg caagcaccat
     1381 gctttctggc gcgtgcattt ctaacagcgc ttgcagctcc tgaaccgtac gaatgccgtc
     1441 cccgccctgg gcatctacgc ggttaacata cggagcaacg tattttgccc ctgccagcgc
     1501 ggccagtaac ccttgtgcag cactgtacac tgcggtgcca agcgtagtaa taccctcttt
     1561 tttcagcatc ttaatggcgg ccagaccttc ggaagtcacc gggattttca ccacaatgcc
     1621 cggaatggcg tcgcgcaggc gcttcgcttc ttccaccata ccctgcgcgt cacgactcat
     1681 agtctgagca aacagaatgc cctcatcacc aatcgctttt tgcagacgcg gcagaacttc
     1741 ccatatggac tccttgctgg cagcgataat gctcgggtta gttgtcaccc cggcaatggg
     1801 gaatatgcgt gccagacgtt cgacttctgc gacgttagcg gtgtccagat acagttccat
     1861 gatgtttcct tttacagttc cagttcatgt tgcagcaggc tggcgatagc gtcggcagag
     1921 gcggcgttaa ccagcgcgtt acggaattct tcgtgcatga tgcggcgagc gaggcgcgag
     1981 aaaatgcgca tgtgctgatc gcccgcagcg tgtttgttca gggttaacat aatgatgaat
     2041 tgcgcttcat catcgcccca gcgcaccggc gcttgcagac gcgctacact gatggtggat
     2101 tgctcaatgt gttctgattt gctgtgggga atggcaaaac taaagcccag accggtagag
     2161 aaaacggcct cgcgcgccca caagtcggct tccagtttac gcggatagcg gcagcggccc
     2221 gccagcagca ggttatcggt catgcctttg agcacttctt ctttgctgcg tcagtcgctt
     2281 tccagcgtga tgcactcggc ggtgaccagc ggtgcgtcct gttgggtcat gcggaattgc
     2341 gccagcaggt gttccacttc cagcgaagta cggcaggcca ttgcctggtt gagcaactgg
     2401 cggcattcgc ggctatcaag ttgcgccatt cgcgctttcg ccgccggaat tgacggtgcg
     2461 ctcatgctca gctcatccag ccctaaaccg accagcaacg gcagcacgga acctttcgct
     2521 cccagctcac cgcacagacc aatccatttg ccctggcgat gcaccgcttg cacggcgtaa
     2581 tcgagcgccc gcaagaatgc cggattcagg ctgttgtagt gacgagtaac cttagcatta
     2641 tcgcgatcca ccgccagcag atattgcgtc aggtcattac taccaatact aaagaaatca
     2701 atctcttcgc agcattgatc gatgatgaac atcaccgacg gcacttccag cataatgccg
     2761 agctggatct tctcatcaaa cggaatgtgt tcgttacgca gttgctgttt ggcttccgcc
     2821 agtttttctt tcacccataa gatctcttcc attgaagaga tcatcgggat catgattttc
     2881 aggctgccgt gagcggaggc gcgcaggatc gaccgtagtt gtgtggtgaa cagcgaggcg
     2941 tattcttcat aaatacgcac agcgcgataa ccgaggaacg ggttagcttc tgccggaatg
     3001 ttcagataat caacgggttt gtcgccgcca atgtccatag tgcgcacaat aatgctgcgt
     3061 ccgttggcag actccagcgc ctgacaaaaa atgttgtaca gctcgctttc gcccggtgca
     3121 ctggtgcgat ccatatagag catttcagtg cggaacaaac caacgccttc cgcaccattg
     3181 ccgaatgcgg cctgcgcttc cacggagtga gcgatgttag cggcaatttc aatgcggata
     3241 ccgtcagcgg tacgggcttg ttgggtcagc cagacacgct gttgctcacg cagggcatcc
     3301 tgtacgcggg cttcttgctg ataataacgg gctacggctt cccctggctc aaccacaatc
     3361 gctccggcgt tgccgtcgat ataaatcgtt tgatgttgcc acggggtaag ggcatcaata
     3421 tccacaccaa ccagcgtcgg aatgttgaac gaacgggcaa ggatcaccgt atgtgaggtg
     3481 gtgccgccgc ttttgagcaa caatcctttg aggtgatttt tatccagttc gaggaactgg
     3541 ctgggggtca gttcatcagc catacaaatg gcgggctgcg tcagtttgcc cggtgccggg
     3601 aagcgttgct caccgtagat ttgctggagt aactggaagc agacgtcgcg tacgtccagg
     3661 gcacgttctt gcaggtagct gctgctggaa cgggaaaact cttcacaaaa gtgattcgca
     3721 ctggcgacaa tcgcttcggc gcagcttaat ccggcgctga cgcctgccag taaatgttcg
     3781 tgcagggaag tatcgccagc cagggatcgg tgcgcttcca gaatcgcgct ggtggcaccg
     3841 tcgctatcca gcagacgaaa ctcaatgttt ttcagtacca gcgtcaggcc gttttccagt
     3901 gcggattgct cggcgtcaac gtctttggct gcgggaagat tactcagcgc attgagatct
     3961 aaagaagaga tcggcgtcag aatgccgcct gcgctaccgc tgcacacggt gcgggcgcgg
     4021 ataatttgcg gattcagatt ggtcagtgaa accggcagtg gttccagttc gtcagattta
     4081 acttccgcca gcggtgcgtc gcagtggggg aattcatcgc gcagccattg gcttaaacgc
     4141 tggtgcgctt cctgttcgtc ggccccggaa atcagtaacc ggtagttatc gcccgccagc
     4201 gtgtcggtgc caatcagcgc aagggcgctt ttggcgttgc ccttgcggtc agtgcgcagg
     4261 ttatgccact caatttgtga tgaaaaagta ttacacagcg tttcaacgtg gcttgccgga
     4321 cgtgcatgta cgccgttagg tagctcacaa ataaattcca caatcagggc cattgcctct
     4381 ctcccggtac ggttttctgt ttacaggata agagcgcacg gcaacggcct gccatgtgac
     4441 aaatctgcca aaagctggac aaatgtaatg taaccgtcaa tttgcgacgc gtctcacaag
     4501 acgccgtttt gcggcatgct tccggattat cgcaagttat gaggcggatc gcatttttgt
     4561 actgatatta caaaagtcca gtaaatggcc tttttatcca ctgtttgctc cgcctgcgat
     4621 tgcctattgt ttcctccatc aaaaatatgg acatgggctt tcgcaagccc gggagcagct
     4681 tatgaatgag ttggtgcaga tcctgaaaaa tacccgtcag catttaatga cgggcgtttc
     4741 acacatgatt cccttcgtgg tatcgggcgg tattttgctg gcggtttccg tcatgttgta
     4801 tggcaaaggc gcagtgccgg atgccgtagc cgatccgaat ctgaaaaaac tgtttgatat
     4861 cggagttgca ggtttgacgc tgatggtgcc tttcctcgcc gcttacatcg gttactccat
     4921 tgcagagcgt tctgcgctgg ccccgtgcgc tatcggtgcc tgggttggta acagctttgg
     4981 tgcgggcttc tttggtgcgc tgatcgccgg gattatcggc ggcatcgtgg tgcattacct
     5041 gaagaaaatt ccggtgcata aagttctgcg ctcggtgatg cctatcttca ttattcctat
     5101 cgtcggcaca ctgattaccg caggcatcat gatgtggggc ttgggcgagc ctgtaggggc
     5161 gttgaccaac agcctgactc agtggcttca ggggatgcag cagggcagca ttgttatgct
     5221 ggcggtgatc atgggtctga tgctggcgtt cgatatgggc ggtccggtta acaaagtggc
     5281 ctatgccttc atgctgattt gcgttgctca gggtgtttat accgtggtgg ccattgctgc
     5341 cgtgggtatt tgtatcccgc cgctggggat ggggctggcg acgctgattg gtcgtaaaaa
     5401 tttctccgca gaagagcgcg aaaccggcaa agcggcgctg gtgatggggt gcgttggggt
     5461 tactgaaggg gcgattcctt tcgccgctgc cgatccgctg cgtgtcattc cttccatcat
     5521 ggtcggttct gtttgtggtg cggtaactgc ggcgctggtc ggtgcgcagt gctatgcagg
     5581 ctggggtggt ctgattgtgc tgccggtggt tgaaggcaag ctgggttata tcgcagcagt
     5641 ggctgtcgga gcagtggtga cggctgtttg cgtgaacgtg ctgaaaagtc tggcgcgtaa
     5701 aaatgggtct tcgactgatg agaaggaaga cgacctggat ttggattttg aaattaatta
     5761 attgaggaac gagccatgac gaaaattatt gcagtaaccg cgtgtccatc tggtgttgcc
     5821 catacctata tggcggcaga ggcgctggaa agcgccgcga aagcgaaagg ctgggaagtg
     5881 aaagtagaaa ctcaggggtc aattggtctg gaaaacgaac tgactgcgga agacgtggcg
     5941 agcgccgata tggtgattct gaccaaagat atcggcatca agtttgaaga gcgttttgct
     6001 ggcaagacca tcgtgcgcgt caacatcagc gatgcggtaa aacgtgctga cgccattatg
     6061 agcaaaattg aagctcacct ggcgcaaacc gcttaagttc ttctcccgct cgcaagggcg
     6121 ggttcgcttt cccacaggag ttcctcatga cgaatcgtat ctctcgcctc aaaactgcac
     6181 tgttttccaa tacccgtgaa atctcgctgg agcgggcgct gctttatacc gccagccatc
     6241 ggcaaaccga aggcgaaccg gtgatattgc gccgggcgaa agcaacagcg tatatccttg
     6301 aacatgttga aatttcgatt cgtgatgaag aactgattgc cggtaaccgc accgtaaaac
     6361 cgcgcgccgg gattatgtcg ccggaaatgg acccttactg gctgctgaaa gagctggatc
     6421 aattcccgac gcgtccgcag gaccgctttg ctatcagcga agaagataaa cgtatctacc
     6481 gcgaagagtt gttcccgtac tgggaaaaac gttcgatgaa agatttcatc aacgggcaga
     6541 tgacagatga agtaaaagcc gcgaccagca cgcagatttt cagcattaac cagacggata
     6601 aaggccaggg gcacattatt attgattacc cacgcctgct gaatcacggg ctgggggagc
     6661 tggtggcgca gatgcagcaa cattgtcagc aacagccgga gaatcacttt tatcaggccg
     6721 cgctgttact gctggaagcc tcgcagaaac acattctgcg ttacgctgaa ctggcggaaa
     6781 caatggcggc aaactgcaca gatgcccagc gtcgcgaaga actgctgact attgcggaga
     6841 tctcccgcca taacgccgaa cataagccgc agacgttctg gcaggcgtgc cagttattct
     6901 ggtacatgaa catcattctg caatacgaat ccaacgccag ttcgctgtct ttagggcgct
     6961 tcgaccagta tatgttgccg ttctatcagg cttctttaac ccagggcgaa gatccggcgt
     7021 tcctgaaaga actgctcgaa tctttatggg tgaaatgcaa cgacatcgtg ctgttgcgct
     7081 ccaccagcag cgcgcgttat ttcgcaggtt tcccgaccgg ctataccgta ctgctcggcg
     7141 ggttaaccga gaacggacgt agcgcggtga acgtgctttc gttcctttgc cttgacgcct
     7201 atcaaagcgt gcaattaccg caaccgaacc tcggcgtgcg cactaacgcc ttgatcgaca
     7261 cgccgttcct gatgaaaacc gccgaaacca ttcgcctcgg caccggtatt ccgcaaatct
     7321 ttaacgatga agtggtggta ccagcgttcc tcaaccgtgg cgtttcgctg gaagatgcgc
     7381 gcgactattc cgtagtgggc tgtgtggaat tatctattcc cggcagaacc tacggcttgc
     7441 atgacatcgc gatgtttaat ctgctgaaag tgatggaaat ctgcctgcat gaaaatgaag
     7501 gcaatgctgc gctgacttat gaaggtttac tggagcagat ccgcgccaag atcagccact
     7561 acatcaccct gatggttgag ggcagcaata tttgtgatat cggccatcgc gactgggcac
     7621 ctgtaccgct gctctcgtct tttatcagcg attgtctgga aaaaggtcgc gatattaccg
     7681 atggcggcgc gcgttataac ttctccggcg tacaggggat cggtatcgcc aacctgagcg
     7741 attctctcca tgcgttgaaa gggatggttt ttgagcaaca gcgtttaagt tttgacgaat
     7801 tgctgtcggt attaaaagcc aactttgcaa cgccagaagg cgaaaaagtc cgtgctcgct
     7861 taattaaccg cttcgagaaa tacggtaacg atatcgacga ggtggataac atcagcgccg
     7921 aactgttacg ccactactgc aaagaagtgg aaaaatacca gaacccgcgc ggtggttact
     7981 tcacgccggg atcgtatacc gtttctgctc acgtcccgtt gggatcggtg gttggcgcga
     8041 cgccagacgg tcgttttgcc ggggaacaac tggcagacgg cggcttgtca cctatgctgg
     8101 gccaggacgc acaagggcca acagcggtac tgaagtcagt aagctcgata atacgctgct
     8161 gtctaacggt acgttgctga acgtgaaatt cactccggcg accctggaag gtgaagcggg
     8221 attacgcaaa ctggccgact tcttacgggc gtttacccag cttaagttgc agcatattca
     8281 gtttaacgtg gtgaacgccg acacgttgcg ggaagcgcaa cagcgcccac aagattatgc
     8341 cgggctggtg gtgcgcgttg ccggatacag cgccttcttt gtcgaactgt cgaaggagat
     8401 ccaggatgac atcatccgcc ggacagcgca tcagctgtaa cgttgtggaa acgcgccgca
     8461 atgatgtggc gcgcattttc aacattcagc gttattcact gaatgacggc gagggcattc
     8521 gtacggtggt cttttttaaa ggctgtccgc atctttgccc atggtgtgct aatccggagt
     8581 cgatctccgg caaaatccag acggtacgca gagaggcgaa atgtctgcac tgtgcgaaat
     8641 gtttgcgtga tgcggatgaa tgcccctccg gggcgtttga acggattggt cgcgatatca
     8701 gccttgacgc gctggaacgg gaagtgatga aagatgacat tttctttcgc acgtccggcg
     8761 gcggcgtcac gctttctggc ggcgaagtgt taatgcaggc ggagtttgct acccgttttt
     8821 tacagcggct gcggttgtgg ggcgtgtcat gcgccattga aactgccgga gacgtgccag
     8881 ccagcaagct gttaccactg gcgaaattgt gcgatgaagt gctgttcgat ttaaaaatta
     8941 tggacgcgac tcaggcgcgg gatgtggtga agatgaacct gccacgcgtg ctggagaatc
     9001 tgcgtttgct ggtgagtgag ggtgtcaacg tgatcccgcg tttaccgttg atccctggtt
     9061 tcacgctcag ccgggagaat atgcagcagg cgctggatgt actgatcccg ctgaaaatca
     9121 ggcagatcca tctgttaccg tttcatcagt acggcgaacc gaaataccgc ctgctgggga
     9181 aaacatggtc gatgaaagag gtgcctgcgc cgtcgtcagc cgatgtgaca acgatgcgcg
     9241 aaatggcaga acgggccgga tttcaggtta ccgtgggagg ttaaaatggc atacctggtg
     9301 gcagtaaccg cctgcgtaag cggcgtggcg catacttata tggcggcgga acggctggaa
     9361 aagttgtgcc agttagagaa gtggggcgtt agcattgaaa ctcagggcgc gctgggaacg
     9421 gagaatcgtt tagcggacga ggatatccgt cgggcggatg ttgctctgtt gattacggat
     9481 atcgagcttg ccggtgccga gcgatttgaa cattgccgct atgtgcaatg cagcatctac
     9541 gcattcctgc gtgagccgca gcgggtaatg agcgcggtgc gcaaagtgct ttctgcgccg
     9601 cagcaaaccc atcttattct ggagtagtcg gtttttctgt cagttggctg tggtactgcc
     9661 gacgatattc cgacggcgag cgttcggtgt ttttacgaaa cagacggcag aagtagttgc
     9721 tgtcgacaaa gccgcaggcg tgcgccactt cttttacctt caggtcatag cctttcaata
     9781 acgtcttagc gtgctccagt cgcgtgtgat tcaggtattc gttaaaacca atggcccccg
     9841 ttttttgaaa caggtgcgag agatagtttg gtgaaatata gaatgcctgt gcgacagatt
     9901 cgcgggtaag cgcggaggca tagcgttcgt cgatataatc gcgaatagct tcaaatagtg
     9961 cctggctgcg tgaggcggtc tggatttggc tgccgagcaa atcgcggcag tggctaagca
    10021 ggctggcgac gataagcctt gccgtttgct gctcctgcgg ctgcatttgc atttcattga
    10081 gcgtttgtag cagaaaagaa cctatgcgtg ggccgcgccg ggcgacgtgt tgcttcgcca
    10141 gattttgata ttgtttgcca tcccattgca cgacgctgaa gccgagttgc tgtttgccaa
    10201 acagcacgct aaaggtagta gcgggggctt gccattgtgg aaaattccag ccaccagcag
    10261 gaacgtacag cacatcgccg ggaactaacg ttgctccagc gccggtatca acaaactcac
    10321 cttccagcac aatttccagc cgtggaaaat cgacctgata cgcgagatcg ggcaccgggc
    10381 cgttactgct ggcaaagtaa atctggcgca gggacagcgg gccgttgatc aggcgggaga
    10441 gcagatagct gacgtcgtga tacatgttat tcccttaggt atgtagacgg gaaaacagta
    10501 actggcatta ttaaaaaagg ccacctgaaa agtgtggcct gaggggagtt cgatgtaaac
    10561 attgcggcag tatcgcatcc ggcaaatagc gccagacgcg atacaaaggt catttactga
    10621 ttacccacct gatcgccata cggcagcgtg tcgtaatcga tcagcgcgtt tttcttgtac
    10681 gggttaccaa tccagcgggt agtttctttg aactgcggat tcaccactga acgggttgga
    10741 tcgtaaccgt cgtaagataa gcctgctaaa tctgaccagg tgtggatcag ttccgccagg
    10801 ctgtatttac gatcaacatc ctgcgagaaa tcacgcggat gagtcgcttg ccatttttcc
    10861 gacgtccaca gcaggaacgg aatggtgtac atgtggcgcg tcgggttgtc ttcgttacgc
    10921 ccctgagttt tgtgcggcgg cgtgtcgtaa acctcttcac cgtggtcaga gaaataaacc
    10981 aggaaaccgt tcgggtttgc tgctttaaag tctttaatca ggctggcaac cacatgatcg
    11041 ttatacaggt tagcgttgtc ataatcgtta tatgactcca gctcttccgc gtttaatcca
    11101 ggcggaacat gatcggtatt gccatcaaac ttgccctgat tttccgggta gcggtatttg
    11161 tatttgatat gcgtacccag cagatgaacg atgatcagtt tcttcggcgc cgggtccttc
    11221 agcacttcct ggaatggttt cagcacgttg gtgtcgtatt cacgcgcact ctgcgtacgt
    11281 tgctggttca tatagaactg tttgtcggtc tgcttcgaaa ataccgtcag catggtattg
    11341 cgggcggtca tcgtctgctg gttggtgatc cagaaggttt tataacctgc ctgtttcatc
    11401 atgttcatca gcgacggttg tgtcaggtac aggtccgggt tcttttcatt agcaaaggtc
    11461 agcgcctgtt gcaggatttc aatggtgtac ggacgagacg taaccacgtt attaaacacg
    11521 gtcagattcg gatcggtttt atgcagcgca tccagctccg gcgtggtttc acgcggataa
    11581 ccgtacagac tcatgcgccc gcgttgggtc gattcgccaa tcaccagcac taaggtacgc
    11641 ggttcgttac ccgattcatc tttgaaatta gccagaggcg gcaaggcatt attttcattc
    11701 agtaactttg ttagcgagtt tagttgctga cgatactgat aatagccggt caggaattgc
    11761 cacggtgcgg caggctccat acgcgaggcc aggttatcca acgttttctc aaacggcttg
    11821 tttttgataa acgtattcat ggcgatcgga tgcagaatca agccataaag cagggcaaaa
    11881 gagacaacat aacgccacgg ctttggaata tagaccgggt gcaggcgtgt ccacagcaga
    11941 actgccaccg ccgtataggc cagcgcgata agcacaattt tcaggctgaa atactggctt
    12001 aaatactcgc tggcttcgtt ggtgttggtt tcgaacatca caaacagaac gctctgcgag
    12061 aactcctgcc cgtagatgac gtagtagcat agcgccgcca gagaggccgc ccatagcacc
    12121 acgccgatta ctgcggcaat aattttaatc cgcttcggaa agaggaatac cgggatcaac
    12181 cacagcgagc tgaataacag cgagtcgcga atgccgttgg tgccactata accactgatg
    12241 taaataatgg cctgtagcag agtagagaaa aaccaaaagt agagcagtgc ccaacccagg
    12301 gctttccagc taaaaagggg tttagcctgg acttctgtgg aatgcatagt aagaacctgt
    12361 cttgaaaaaa atatcgccga atgtaacgac aattccttaa ggatatctga aggtatattc
    12421 agaatttgaa taaaatgcag acagaaatat attgaaaacg agggtgttag aacagaagta
    12481 tttcagaaaa ccctcgcgca aaagcacgag ggtttgcaga agaggaagat tagccggtat
    12541 tacgcatacc tgccgcaatc ccggcaatag tgaccattaa cgcttgttcg acgcgaggat
    12601 ccggttcctg gccttctttt tctgcctggc gggagcggtg cagtaactcg gcctgcaata
    12661 cgttcagcgg gtcggtgtaa atattccgta gctgaataga ctctgcaatc cacggcagat
    12721 cggccattag atgggaatcg ttggcaatcg ccagcaccac tttgatgtct tcttcttgca
    12781 ggttgcgtaa ctctttacct aacggccaca gtgctttgtc taccaggcgt tggtcatagt
    12841 attccgccag ccacaggtct gctttggcga agaccatctc cagcatgccg agacgcgtcg
    12901 agaagaatgg ccaatcgcgg cacatggctt ccagctcgct ctgtttgcca tcttcgacca
    12961 ctttttgcag cgccgtacct gcacccagcc aggcggggag catcaggcgg ttttgcgtcc
    13021 aggcaaaaat ccacggtatg gcgcgcagcg actcgacgcc gccggttggg cgacgtttcg
    13081 ccggacgtga acccaacggc agtttgccca attcttgttc cggcgtagcg gagcggaagt
    13141 aaggcacaaa atctttgttt tcacgtacgt agccacggta gagatcgcag gagatgactg
    13201 acagttcatc cataatgcga cgccagctct ctttcggctc aggtggtggc agcaggttgg
    13261 cttccagaat agccccggtg tacagcgaca ggctgctgac ggtgatttct ggcagaccat
    13321 atttaaagcg gatcatctcg ccctgttcag tcacgcgcag gccacctttc aggcttcctg
    13381 gcggttgtga cagcagcgcc gcatgagcag gtgcgccgcc gcgaccaatg gaaccgccgc
    13441 gaccgtggaa caacgtcagc tcaatacccg ctttttcgca ggttttgatt aatgcatcct
    13501 gtgcctgata ttgcgcccag gaagctgcca tcactcccgc atcttttgct gagtcggaat
    13561 agccaatcat caccatctgt ttgccctgaa tcaggccacg ataccagtca atattgagca
    13621 gctgggtcat gacatcgttg gcgttgttca gatcatcgag ggtttcaaac agcggagcaa
    13681 ccggcatcgc aaacccgata cccgcttctt tcagcagcag gtggacagcc agtacgtcgg
    13741 acggcgtttt cgccatcgag atcacgtagg cggcaatgga gccttgcggt gcttcggcaa
    13801 tcacctggca ggtatcgagc acttcgcgcg tttcggcgct tggttgccag ttgcgcggca
    13861 gaagcggacg tttggagttc agttcgcgga tcaggaacgc ctgtttgtcg gcctctgacc
    13921 agctttcgta gtcgccgata ccgaggtagc gggtcagctc gcccagcgct tcggtatgac
    13981 gcgtgctctc ctgacggata tcaatacgga ccagcggtac gccgaaacat ttcacgcggc
    14041 gcagggtgtc gagcagatcg ccgttggcga taatacccat gccacacgcc tgaagtgact
    14101 ggtagcaagc gtagagcggt tcccacagtt cttcgttttg tgtcagcagg ccttctggtt
    14161 ttggcagttc ttcgcctttc aggcgcgctt ccagccatgc ctgtgtcgcc atcaggcgag
    14221 aacgcaggtt tttcatcaga tagcgatacg gttctgcggc accttcttcg ccaaccagcg
    14281 ccagcagttc aggggtcgct tcaaccatcg acagttcaga aaccagcacc tgaatatctt
    14341 tcaggaacaa atcggtggct ttccagcggc tgagtagcag gacgtggcgg gtgatatcgg
    14401 cagtgacgtt cgggttgccg tcgcggtcgc cgcccatcca cgaagtaaaa cggaccggaa
    14461 caaattcgac gggcagtttg tagccgaggt tctcttccag ttgttcgttc agttcgcgca
    14521 ggtaatttgg tacgccttgc cacaggctgt tttccactac ggcaaagccc catttggctt
    14581 catctaccgg gcttggacgc agcttacgga tttcatcggt atgccatgac tgggcgatta
    14641 actggcgcag gcggcgcatc agctggttgt gttcgtagtc agcgatatct ttgttatcga
    14701 gctgttttaa acaggcgttc acttccacca ttttgtggat cagtgtacga cgggtaattt
    14761 cggttgggtg agccgtgagg accagttcca gcgacagcga ttccactgct tttttgatgg
    14821 tgtcttcgct cagttccggc tggtttttca gtttacgcag ggtgcgggcg atcacttccg
    14881 ggttgctggc agcttcgcct ttcggcgaaa tgctgtggta ttgctcggcg gtgttggcca
    14941 ggttcaggaa ctgactaaac gcacgcgcaa cgggcagcag ctcgtcgttc gacaaatttt
    15001 gtaaggtggt gagcaactcc tggcggttag catcattgcc agcgcgtgaa gatttcgaca
    15061 acttacggat agtttctacg cgttcaagaa tgtgttctcc caacgcatcc ttgatggttt
    15121 ctcccagcac tttgccgagc atactgacat tactacgcaa tgcggaatat tgttcgttca
    15181 tattacccca gacaccccat cttatcgttt gatagccctg tatccttcac gtcgcattgg
    15241 cgcgaatatg ctcgggcttt gcttttcgtc gtcttttata aagccacgta aaagcggtga
    15301 cgtcaaatgc tgcgaaatcg cttcagcaaa cgaataaata gcaggaattt acgccattaa
    15361 attcacgacg ctttaaataa gcgtaactta tggaaatgtt aaaaaatcgc catacataac
    15421 accaaaggtg taggtcggat aagatgcgca agcatcgcat ccgacattat tgcggcacag
    15481 gagtttggca acggtgccgg atgcggcgcg agcgccttat ccggcctacg ttttaatgcc
    15541 agcaaaaatg gtgaattacc tgggttatca gttcgcgggt gggcttgata aaccgtgttt
    15601 ccagatattc atcaggttga tgagcctgat taattgagcc aggccccaac accagcgtcg
    15661 ggcataacgt ttgaataaac ggcgcttcgg tacagtagtt caccacttcg gtctttgctc
    15721 cgagcaattt ctcaactact tcaaccagtt gatgattcgg tgggcattca tagccaggga
    15781 tcggcggatg cagctcgtcg accgtcagac gacccggcca gcgttcgctc accggagcca
    15841 atgcatcgtt gagcaaacca ttaagttcat tgagtgtcat gccaggcagc ggacgaatat
    15901 ccatatgcaa ctcacagcaa gcgcaaatac ggttagaagc gtcgccaccg tgaatatgcc
    15961 cgaggttgag cgtagggtat ggcacggtaa acgcttcgta gtgataacgt tctttcaggt
    16021 tatcgcgcaa ttgcaaaata tgcccgatgg cgtcgtgcat tagttcgata gcgttaactc
    16081 cgcgtgctgg atcgctggag tgccccgact ggccctgaat acggatggcg ttagagatat
    16141 gacctttatg tgcgcgtacc ggttgtagtg acgtcggttc gccaatgatg gcgcaatccg
    16201 ggcgcagggc ggtagtttcg gcaaaataac gcgctccggc catactggtt tcttcatcag
    16261 cagtcgccag aatgtagagc ggttttttca gtttcgtgac gtcgacatcg cgtagcgcat
    16321 caaggataaa cgcaaaaaag cctttcatgt cggcggtgcc taagccgtaa agcttgccgt
    16381 catgctccgt cagtgtaaac ggatcgcgcg tccagcgacc gtcatcaaat ggcaccgtat
    16441 cggtatgccc cgccagcaac aagccgccag ccccctgtcc gatacttgcc agcatattga
    16501 atttgttgcg agttcctggc acaggctgca cttccacatt gaagcctaaa tctttaaacc
    16561 agtccgccag cagagtgatt aaatctgcat tgctttgatc gagtgcctct tccgtggcgc
    16621 ttattgaagg tgtggcaatc agagcacggt aaatctcgat aaatggcggt aatttgtttt
    16681 tcattgttga cacacctctg gtcatgatag tatcaatatt catgcagtat ttatgaataa
    16741 aaatacacta acgttgagcg taataaaacc caccagccgt aaggtgaatg ttttacgttt
    16801 aacctggcaa ccagacataa gaaggtgaat agccccgatg ttgaatacgc tgattgtggg
    16861 tgccagcggc tacgctggcg cagagctagt gacctatgta aatcgccatc cgcatatgaa
    16921 cataaccgct ttgactgttt cagcgcaaag caatgatgcg ggaaagttaa tctccgattt
    16981 gcatccgcag ctaaaaggca tcgttgatct gccgttgcag ccgatgtcgg atatcagcga
    17041 gtttagccca ggggtggacg tagtgtttct cgccaccgcc catgaagtta gccacgattt
    17101 agcgccgcag tttcttgaag cgggctgcgt ggtgttcgac ctttccggcg cgtttcgtgt
    17161 taacgacgcc accttctatg aaaaatatta cggctttacc catcaatacc cggaactgtt
    17221 ggaacaggca gcctacggtc tggcggagtg gtgcggtaat aaattaaaag aagcgaattt
    17281 gattgcggtg ccgggctgtt atccgacggc ggcacagctg gcgctgaaac cgttgattga
    17341 tgccgatctt cttgacctca atcagtggcc ggtgatcaac gccaccagcg gcgtgagcgg
    17401 tgcaggacgt aaggcagcga tttcaaatag tttttgtgaa gttagcctgc aaccgtatgg
    17461 cgtctttacc catcgccatc aaccagagat cgccacacac ctcggtgctg acgttatctt
    17521 caccccgcat ctgggcaatt tcccgcgcgg cattcttgaa accattacct gccgcctgaa
    17581 accgggtgtg acccaggcgc aagtggcgca agcgttacag caggcgtatg cccataaacc
    17641 gctggtgcgg ctgtatgaca aaggcgttcc ggcgctgaaa aatgtcgttg ggctgccatg
    17701 ttgcgatatt gggtttgccg ttcagggcga gcatctgatt attgtggcga ccgaagacaa
    17761 cttactgaaa ggcgcggcgg cacaagcggt acagtgcgcc aatattcgtt tcggctatgc
    17821 ggaaacgcag tctcttattt aagggtgcaa tgatgaatcc attaattatc aaactgggcg
    17881 gcgtactgct ggatagtgaa gaggcgctgg aacgtctgtt tagcgcactg gtgaattatc
    17941 gtgagtcaca tcagcgtccg ctggtgattg tgcacggcgg cggttgcgtg gtggatgagc
    18001 tgatgaaagg gctgaatctg ccggtgaaaa agaaaaatgg cctgcgggtg acgcctgcgg
    18061 atcagataga cattatcacc ggagcactgg caggaacggc aaataaaacc ctgctggcat
    18121 gggcgaagaa acatcagatt gcggccgtag gtttgtttct cggtgacggc gacagcgtca
    18181 aagtgaccca gcttgatgaa gagttaggtc atgttggact ggcgcagcca ggttcgccta
    18241 agcttatcaa caccttgctg gagaacggct atctgccggt ggtcagctcc attggcgtaa
    18301 cagacgaagg gcaactgatg aacgtcaatg ccgaccaggc ggcgacggcg ctggcggcaa
    18361 cgctgggcgc ggatctgatt ttgctctctg atgtcagcgg cattctcgac ggcaaagggc
    18421 aacgcattgc cgaaatgacc gccgcgaaag cagaacaact gattgagcag ggcattatta
    18481 ctgacggcat gatagtgaaa gtgaacgcgg cgctggatgc tgcgcgcacg ttgggccgtc
    18541 cggtagatat cgcctcctgg cgtcatgcgg aacagcttcc ggcactgttt aacggtatgc
    18601 cgatgggtac gcggatttta gcttaagttt tgttggccgg aggcgcagct ttccggcatt
    18661 gaatttcaaa ataaggaaac agagttatgg cactttgggg cgggcgtttt acccaggcag
    18721 cagatcaacg gttcaaacaa ttcaacgact cactgcgctt tgattaccgt ctggcggagc
    18781 aggatattgt tggctctgtg gcctggtcca aagccctggt aacggtcggc gtgttaaccg
    18841 cagaagagca ggcgcaactg gaagaggcgc tgaacgtgtt gctggaagat gttcgcgcca
    18901 ggccacaaca aatccttgaa agcgacgccg aagatatcca tagctgggtg gaaggcaaac
    18961 tgatcgacaa agtgggccag ttaggcaaaa agctgcatac cgggcgtagc cgtaatgatc
    19021 aggtagcgac agacctgaaa ctgtggtgca aagataccgt tggcgagttg ctgacggcta
    19081 accggcagct gcaatcggcg ctggtggaaa ccgcacaaaa caatcaggac gcggtgatgc
    19141 caggttacac tcacctgcaa cgcgcccagc cggtgacgtt cgcgcactgg tgcctggcct
    19201 atgttgagat gctggcgcgt gatgaaagcc gtttgcagga tgcgcttaag cgtctggatg
    19261 tcagcccatt aggctgtggc gcgctggcgg gaacggccta tgaaatcgac cgtgaacagt
    19321 tagcaggctg gctgggcttt gcttcggcga cccgtaacag tctcgacagc gtttctgacc
    19381 gtgaccatgt gttggaactg ctttctgctg ccgctatcgg catggtgcat ctgtcgcgtt
    19441 ttgctgaaga tctgattttc tttaacaccg gcgaagcggg gtttgtggag ctttctgacc
    19501 gcgtgacttc cggttcatca ttaatgccgc agaagaaaaa cccggatgcg ctggagctga
    19561 ttcgcggtaa atgcggtcgg gtgcaggggg cgttaaccgg catgatgatg acgctgaaag
    19621 gtttgccgct ggcttacaac aaagatatgc aggaagacaa agaaggtctg ttcgacgcgc
    19681 tcgatacctg gctggactgc ctgcatatgg cggcgctggt gctggacggc attcaggtga
    19741 aacgtccacg ttgccaggaa gcggctcagc agggttacgc caacgccacc gaactggcgg
    19801 attatctggt ggcgaaaggc gtaccgttcc gcgaggcgca ccatattgtt ggtgaagcgg
    19861 tggtggaagc cattcgtcag ggcaaaccgc tggaagatct gccgctcgac gagttgcaga
    19921 aattcagccc ggtgattgac gaagatgtct atccgattct gtcgctgcaa tcgtgcctcg
    19981 acaagcgtgc ggcaaaaggc ggcgtctcac cgcagcaggt ggcgcaggca attgcttttg
    20041 cgcaggcgcg gttggggtaa gaacatttat atgtataaat ttgagcctgg cttatcgccg
    20101 ggctttttta tggcaaaaaa aagcggatcc tggagatccg caaaagttca cgttggcttt
    20161 agttattcga gttgagaaac tctcgaaacg ggcagtgact tcaagggtta aaagaggtgc
    20221 cgctccgttt ctgtgagcaa ttatcagtca gaatgcttga tagggataat cgttcattgc
    20281 tattctacct atcgccatga actatcgtgg caatggagga tggataatga atattcgtga
    20341 tcttgagtac ctggtggcat tggccgaaca ccgccatttt cggcgtgcgg cagattcctg
    20401 ccacgttagc cagccgacgc ttagcgggca aattcgtaag ctggaagatg agttgggcgt
    20461 gatgttgctg gagcggacca gccgtaaagt gttgttcacc caggcgggaa tgctgctggt
    20521 agatcaggcg cgtaccgtac tgcgtgaggt gaaagtcctt aaagagatgg caagccagca
    20581 gggcgagacg atgtccgggc cgctgcacat tggtttgatt cccacagttg gaccgtacct
    20641 gctaccgcat attatcccta tgctgcacca gacctttcca aagctggaaa tgtatctgca
    20701 tgaagcacag acccaccagt tactggcgca actggacagc ggcaaactcg attgcgtgat
    20761 cctcgcgctg gtgaaagaga gcgaagcatt cattgaagtg ccgttgtttg atgagccaat
    20821 gttgctggct atctatgaag atcacccgtg ggcgaaccgc gaatgcgtac cgatggccga
    20881 tctggcaggg gaaaaactgc tgatgctgga agatggtcac tgtttgcgcg atcaggcaat
    20941 gggtttctgt tttgaagccg gggcggatga agatacacac ttccgcgcga ccagcctgga
    21001 gacgctgcgc aacatggtgg cggcaggtag cgggatcact ttactgcctg cgctggctgt
    21061 gccgccggag cgcaaacgcg atggggttgt ttatctgccg tgcattaagc cggaaccacg
    21121 ccgcactatt ggcctggttt atcgtcctgg ctcaccgctg cgcagccgct atgagcagct
    21181 ggcagaggcc atccgcgcac gaatggatgg ccatttcgat aaagttttaa aacaggcggt
    21241 ttaaaccgtt taacgcagct acccgatagg cttccgccat cgtcgggtag ttaaaggtgg
    21301 tgttgacgaa gtactcaata gtgttgccgc cacctttctg ttccataatc gcctgaccga
    21361 tatgaataat ttcggcagcg cgctcgccaa agcagtgaat acccagaatc tcttttgttt
    21421 cccgatggaa caaaattttc agcgtgccca cgttcatgcc gacgatttgt gcgcgtgcca
    21481 gatgtttaaa ctgggcgcgg cccacttcat atggcacttt cattgcggtc agctgctgtt
    21541 cggttttgcc cacagagctg atttccggga tggtgtaaat gccggtaggg atatcttcaa
    21601 tcagatgtgc ggtggcttcg ccttttacca gcgcctgcgc ggcaatgcgc ccctggtcat
    21661 aggccgctga tgccaggctc ggataaccaa tcacgtcgcc caccgcgtaa acgtgcggct
    21721 gtgcggtctg atacatgctg ttgaccttca gctgtccgcg gctgtcagtt tccagcccaa
    21781 tgttctgtaa cgccagcgaa tcggtattac cggtgcgacc gttggcatag agcaggcagt
    21841 cagctttcag ttttttaccc gacttcaaat gcatgatcac accatcgtca cagccttcga
    21901 tcttctcgta ctcttcgttg tgacgaatca ctacgccact gttccagaag tgataggaga
    21961 gagaatctga catctcttga tcgagaaatg ccagcaggcg atcgcgggtg ttgatcagat
    22021 ccacttttac atccataccg cggaagatcg acgcatattc acagccgatc actccagcac
    22081 catagataag tacatggcgc ggttcgtggt gcatgctaag aattgagtcg ctgtcgtaaa
    22141 tgcgtggatg ggtgaaatca acatctgttg gatgatatgg acgagagccg caggcaataa
    22201 caaatttttc agcggttagt gtttcaacgc tgccgtccgg gcaatccagc gccaacgtat
    22261 gctcgtcaac aaagcgagcg tttccctgca atatttcaca gtgattacgt tcgtaaaatc
    22321 cctgacgcat gcgcgtttgt tgattaatca cgttatcggc atggttaagg atatcggcaa
    22381 aagaagagcg gagcagtcgg gaatggtcgc tgtaaagtgg gttttgattg aattctataa
    22441 tgcggctgac ggcgtgacgg agagctttcg acgggatggt gccccagtgg gtgcaaccgc
    22501 cgccaacatt ttgataacgc tcgataactg cgacgcgcgc accttgctta accaggccca
    22561 ttgcagcgcc ttcgccgccg gggccggaac ctattactat ggcatcgtaa tcgtaggaat
    22621 gtggcatggt agggcttacc tgttcttata cataaaagca acagaatggt aacattttat
    22681 cgcgggtaag ccaattgatc cccgtcattt atctggctat atcctgagcg gcctttgctt
    22741 tgtctgtttc ttacttttgc cctgacgttt tattggattt ttatcgacga tactctccgt
    22801 ttaagcggca ggtttccgct gtacgtaaaa gaaccggcca aagaattgca gtaaatatgt
    22861 tttattgcgt taccgttcat tcacaatact ggagcaatcc agtatgttca ttctctggta
    22921 tagtgccagc agtacttttg gcaaggattc agacatcgtg atgggcgtaa gagcgcaaca
    22981 aaaagaaaaa acccgccgtt cgctggtgga agccgcattt agccaattaa gtgctgaacg
    23041 cagcttcacc agcctgagtt tgcgtgaagt ggcgcgtgaa gcgggcattg ctcccacctc
    23101 tttttatcgg catttccgcg acgtagacga actgggtctg accatggttg atgagagcgg
    23161 tttaatgcta cgccaactca tgcgccaggc gcgtcagcgt atcgccaaag gcgggagtgt
    23221 gatccgcacc tcggtctcca catttatgga gttcatcggt aataatccta acgccttccg
    23281 gttattattg cgggaacgct ccggcacctc cgctgcgttt cgtgccgccg ttgcgcgtga
    23341 aattcagcac ttcattgcgg aacttgcgga ctatctggaa ctcgaaaacc atatgccgcg
    23401 tgcgtttact gaagcgcaag ccgaagcaat ggtgacaatt gtcttcagtg cgggtgccga
    23461 ggcgttggac gtcggcgtcg aacaacgtcg gcaattagaa gagcgactgg tactgcaact
    23521 gcgaatgatt tcgaaagggg cttattactg gtatcgccgt gaacaagaga aaaccgcaat
    23581 tattccggga aatgtgaagg acgagtaatg aaacaagcaa atcaagatag aggtacgctg
    23641 ctgctggcgt tagttgctgg cttatcgatt aatggtactt tcgcagcgct gtttagctcc
    23701 attgtgccat tttctgtatt cccgattatt tccctggtgc tgacggttta ctgcctgcat
    23761 caacgttatc ttaatcgcac tatgccggta ggcttgccgg gtctggcagc tgcctgtttt
    23821 attctcggcg tactgctgta cagcacggta gttcgtgcgg aatatccgga tatcggctct
    23881 aacttcttcc cggcagtact ctccgtcatt atggtgttct ggattggcgc gaagatgcgt
    23941 aaccgtaagc aggaagttgc tgaataatcg gtattatgcc ggatgctggc gcatccagca
    24001 tgggttttac ttcgcggtca gtaatacgcc gcactccata tggtgcgtgt aggggaactg
    24061 atcaaacaga gccagacgtt cgaccttgtg cgtctggctt aatgtttcca gattcttgca
    24121 taacgtttcc gggttacagg agatgtacaa aatacacgga tacgcctgca ccattttctc
    24181 ggtttcactg tccagaccgc tgcgcggtgg gtcgacaaaa atggtttcgc actgataact
    24241 ctttaagtcg atcccttgca ggcggttaaa ctcgcgcaca ccattcatcg cctgagtaaa
    24301 ttcttctgcc gccatacgaa taatttgtac gttatcaata tggttagctg cgatgttgta
    24361 ttgcgcagca gcaaccgacg gcttagcgat ttcggtggct aatacccgat caaaattgcg
    24421 cgccagcgct aatgaaaagt taccgttgcc gcagtacagc tccagtaaat cgccttttga
    24481 gcctttggtt acgtccagcg cccattccag catctgaata ttcatcgccg cgttcggctg
    24541 ggtaaagctg ttttctacct gacggtagat catctctttc cctgcgaccg gcagacgctc
    24601 gtcgatgtaa tcttgatcca gctcgatttt ggttttcgtt gcccgaccaa tcagatgcac
    24661 attcagattc tgcgcgcgca gtgcatcgcg cagggcctcc gcttcctgac gccactcatc
    24721 atccagcttc ttatggtata gcagggaaac caccgcctga ttactcagcg tggtgaggta
    24781 atcaatctgg aacaacttgt ggcgcagaac gggattatta cgcacacccg caatcatcgc
    24841 cgtcatcaac tggttgataa gttcactggc ggcggggaag ctatccacgc ggatgcggct
    24901 tttggtttgt tgatcgaaaa tgatgtgata caggtcatcg ccatcgtgcc agatgcggaa
    24961 ctccgcgcgc atccggtaat gactgaccgg cgagcgaaac acttccggaa ccaggtcaga
    25021 aaacggtgcc atcatacttt gcaaacgtac cactttttcg gctaactgcg cttcatactg
    25081 ttctgttgga aggtgttcgg gggtcatgat gtatcctgaa aaattaaagt acgcggcgat
    25141 tgtagggatt gctcatcaga tgtccagatc ttgatgaatt cctatttgtg agctacgtct
    25201 ggacagtaac ttgttacaac ctgtagcatc cacttgccgg tcctgtgagt taatagggaa
    25261 tccagtgcga atctggagct gacgcgcagc ggtaaggaaa ggtgcgatga ttgcgttatg
    25321 cggacactgc catccggtgg gaagtcatca tctcttagta tcttagatac ccctccaagc
    25381 ccgaagacct gccggccaac gtcgcatctg gttctcatca tcgcgtaata ttgatgaaac
    25441 ctgcggcatc cttcttctat tgtggatgct ttacaatgat taaaaaagct tcgctgctga
    25501 cggcgtgttc tgtcacagcc ttttccgctt gggcacagga taccagcccg gatactctcg
    25561 tcgttactgc taaccgtttt gaacagccgc gcagcactgt gcttgcacca accaccgttg
    25621 tgacccgtca ggatatcgac cgctggcagt cgacctcggt caatgatgtg ctgcgccgtc
    25681 ttccgggcgt cgatatcacc caaaacggcg gttcaggtca gctctcatct atttttattc
    25741 gcggtacaaa tgccagtcat gtgttggtgt taattgatgg cgtacgcctg aatctggcgg
    25801 gggtgagtgg ttctgccgac cttagccagt tccctattgc gcttgtccag cgtgttgaat
    25861 atatccgtgg gccacgctcc gccgtttatg gttccgatgc aataggcggg gtggtgaata
    25921 tcatcacgac gcgcgatgaa cccggaacgg aaatttcagc agggtgggga agcaatagtt
    25981 atcagaacta tgatgtctct acgcagcaac aactggggga taagacacgg gtaacgctgt
    26041 tgggcgatta tgcccatact catggttatg atgttgttgc ctatggtaat accggaacgc
    26101 aagcgcagac agataacgat ggttttttaa gtaaaacgct ttatggcgcg ctggagcata
    26161 actttactga tgcctggagc ggctttgtgc gcggctatgg ctatgataac cgtaccaatt
    26221 atgacgcgta ttattctccc ggttcaccgt tgctcgatac ccgtaaactc tatagccaaa
    26281 gttgggacgc cgggctgcgc tataacggcg aactgattaa atcacaactc attaccagct
    26341 atagccatag caaagattac aactacgatc cccattatgg tcgttatgat tcgtcggcga
    26401 cgctcgatga gatgaagcaa tacaccgtcc agtgggcaaa caatgtcatc gttggtcacg
    26461 gtagtattgg tgcgggtgtc gactggcaga aacagactac gacgccgggt acaggttatg
    26521 ttgagaatgg atatgatcaa cgtaataccg gcatctatct gaccgggctg caacaagtcg
    26581 gcgattttac ctttgaaggc gcagcacgca gtgacgataa ctcacagttt ggtcgtcatg
    26641 gaacctggca aaccagcgcc ggttgggaat tcatcgaagg ttatcgcttc attgcttcct
    26701 acgggacatc ttataaggca ccaaatctgg ggcaactgta tggcttctac ggaaatccga
    26761 atctggaccc ggagaaaagc aaacagtggg aaggcgcgtt tgaaggctta accgctgggg
    26821 tgaactggcg tatttccgga tatcgtaacg atgtcagtga cttgatcgat tatgatgatc
    26881 acaccctgaa atattacaac gaagggaaag cgcggattaa gggcgtcgag gcgaccgcca
    26941 attttgatac cggaccactg acgcatactg tgagttatga ttatgtcgat gcgcgcaatg
    27001 cgattaccga cacgccgttg ttacgccgtg ctaaacagca ggtgaaatac cagctcgact
    27061 ggcagttgta tgacttcgac tggggtatta cttatcagta tttaggcact cgctatgata
    27121 aggattactc atcttatcct tatcaaaccg ttaaaatggg cggtgtgagc ttgtgggatc
    27181 ttgcggttgc gtatccggtc acctctcatc tgacagttcg tggtaaaata gccaacctgt
    27241 tcgacaaaga ttatgagaca gtctatggct accaaactgc aggacgggaa tacaccttgt
    27301 ctggcagcta caccttctga accacgtccc accgtgctgg tgtttgactc cggcgtcggt
    27361 gggttgtcgg tctatgacga gatccggcat ctcttaccgg atctccatta catttatgct
    27421 ttcgataacg tcgcttttcc gtatggcgaa aaaagcgaag agtttattgt tgagcgagtg
    27481 gtggcaattg tcaccgcagt gcaagaacgt tatccccttg cgctggctgt ggtcgcttgc
    27541 aacaccgcca gtaccgtttc acttcctgca ttacgcgaaa agttcgactt cccggttgtt
    27601 ggtgtcgtgc cggcgattaa acctgctgca cgtctgacgg caaatggcat tgtcggatta
    27661 ctggcaaccc gcggaactgt taaacgttct tatactcatg agctgatcgc gcgtttcgct
    27721 aatgaatgcc agatagaaat gctgggctcg gcagagatgg ttgagttggc tgaagcgaag
    27781 cttcatggcg aagatgtttc tctggatgca ctaaaacgta tcctacgccc gtggttaaga
    27841 atgaaagagc cgccagatac cgttgtattg ggttgcaccc atttccctct actacaagaa
    27901 gaactcttac aggtgctgcc agagggaacc cggctggtgg attctggcgc agcgattgct
    27961 cgcagaacgg cctggctgtt agaacatgaa gctccggatg caaaatctgc cgatgcgaat
    28021 attgcctttt gtatggcaat gacgccagaa gctgaacaat tattgcccgt tttacagcgt
    28081 tacggcttcg aaacgctcga aaaactggca gttttaggct gatttggttg aatgttgcgc
    28141 ggtcagaaaa ttattttaaa tttcctcttg tcaggccgga ataactccct ataatgcgcc
    28201 accactgaca cggaacaacg gcaaacacgt cgccgggtca gcggggttct cctgagaatc
    28261 tcaacagaga aaagcaaaaa atgcttgact ctgtagcggg aaagcgtatt atgcacaccc
    28321 cgcgccgctg ag
//
LOCUS       DAZKHF020000033        28011 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2201S31241
            SAMN43267800-rid25106413.denovo.033, whole genome shotgun sequence.
ACCESSION   DAZKHF020000033 DAZKHF020000000
VERSION     DAZKHF020000033.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267800
            Sequence Read Archive: SRR30307263
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 28011)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 28011)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 28011)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350581.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350581.2
            Genome Coverage       :: 151x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:21:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,652
            CDSs (total)                      :: 5,557
            Genes (coding)                    :: 5,320
            CDSs (with protein)               :: 5,320
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 237
            CDSs (without protein)            :: 237
            Pseudo Genes (ambiguous residues) :: 0 of 237
            Pseudo Genes (frameshifted)       :: 102 of 237
            Pseudo Genes (incomplete)         :: 130 of 237
            Pseudo Genes (internal stop)      :: 52 of 237
            Pseudo Genes (multiple problems)  :: 41 of 237
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28011
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267800-rid25106413.denovo.033"
                     /serotype="O 26:H11"
                     /isolate="2201S31241"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-01"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            85..636
                     /gene="maa"
                     /locus_tag="ACHOSC_RS05835"
                     /old_locus_tag="pgaptmp_001167"
     CDS             85..636
                     /gene="maa"
                     /locus_tag="ACHOSC_RS05835"
                     /old_locus_tag="pgaptmp_001167"
                     /EC_number="2.3.1.79"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016407 - acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="maltose O-acetyltransferase"
                     /protein_id="HGU4547144.1"
                     /translation="MSTEKEKMIAGELYRSADETLSRDRLRARQLIHRYNHSLAEEHT
                     LRQQILADLFGQVTEAYIEPTFRCDYGYNIFLGNNFFANFDCVMLDVCPIRIGDNCML
                     APGVHIYTATHPIDPVARNSGAELGKPVTIGNNVWIGGRAVINPGVTIGDNVVVASGA
                     VVTKDVPDNVVVGGNPARIIKKL"
     gene            752..1222
                     /gene="ylaC"
                     /locus_tag="ACHOSC_RS05840"
                     /old_locus_tag="pgaptmp_001168"
     CDS             752..1222
                     /gene="ylaC"
                     /locus_tag="ACHOSC_RS05840"
                     /old_locus_tag="pgaptmp_001168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308538.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlaC family protein"
                     /protein_id="HGU4547145.1"
                     /translation="MTEIQRLLTETIESLNTREKRDNKPRFSISFIRKHPGLFIGMYV
                     AFFATLAVMLQSETLSGSVWLLVVLFILLNGFFFFDVYPRYRYEDIDVLDFRVCYNGE
                     WYNTRFVPAALVEAILNSPRVADVHKEQLQKMIVRKGELSFYDIFTLARAESTS"
     gene            1386..2936
                     /gene="pdeB"
                     /locus_tag="ACHOSC_RS05845"
                     /old_locus_tag="pgaptmp_001169"
     CDS             1386..2936
                     /gene="pdeB"
                     /locus_tag="ACHOSC_RS05845"
                     /old_locus_tag="pgaptmp_001169"
                     /EC_number="3.1.4.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308537.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic-guanylate-specific phosphodiesterase
                     PdeB"
                     /protein_id="HGU4547146.1"
                     /translation="MRTRHLVGLISGVLILSVLLPVGLSIWLAHQQVETSFIEELDTY
                     SSRVAIRANKVATQGKDALQELERWQGAACSEAHLMEMRRVSYSYRYIQEVVYIDNNV
                     PQCSSLEHESPPDTFPEPGKISKDGYRVWLTSHNDLGIIRYMVAMGTAHYVVMIDPAS
                     FIDVIPYSSWQIDAAIIGNAHNVVITSSDEIAQGIITRLQKTPGEHIENNGIIYDILP
                     FPEMNISIITWASTKMLQKGWHRQVFIWLPLGLVIGQLAAMFVLRILRRIQSPHHRLQ
                     DAIENRDICVHYQPIVSLANGKIVGAEALARWPQTDGSWLSPDSFIPLAQQTGLSEPL
                     TLLIIRSVFEDMGDWLRQHPQQHISINLESTVLTSEKIPQLLREMINHYQVNPRQIAL
                     ELTEREFADPKTSAPIISRYREAGHEIYLDDFGTGYSSLSYLQDLDVDILKIDKSFVD
                     ALEYKNVTPHIIEMAKTLKLKMVAEGIETSKQEEWLRQHGVHYGQGWLYSKALPKEDF
                     LRWAEQHL"
     gene            complement(2978..3331)
                     /gene="ybaA"
                     /locus_tag="ACHOSC_RS05850"
                     /old_locus_tag="pgaptmp_001170"
     CDS             complement(2978..3331)
                     /gene="ybaA"
                     /locus_tag="ACHOSC_RS05850"
                     /old_locus_tag="pgaptmp_001170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1428 domain-containing protein"
                     /protein_id="HGU4547147.1"
                     /translation="MKYVDGFVVAVPADKKDAYREMAAKAAPLFKEFGALRIVECWAS
                     DVPDGKVTDFRMAVKAEENEEVVFSWIEYPSKEVRDAANQKMMSDPRMKEFGESMPFD
                     GKRMIYGGFESIIDE"
     gene            complement(3451..3547)
                     /gene="ffs"
                     /locus_tag="ACHOSC_RS05855"
                     /old_locus_tag="pgaptmp_001171"
     ncRNA           complement(3451..3547)
                     /ncRNA_class="SRP_RNA"
                     /gene="ffs"
                     /locus_tag="ACHOSC_RS05855"
                     /old_locus_tag="pgaptmp_001171"
                     /product="signal recognition particle sRNA small type"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00169"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00169"
     gene            3709..4020
                     /locus_tag="ACHOSC_RS05860"
                     /old_locus_tag="pgaptmp_001172"
     CDS             3709..4020
                     /locus_tag="ACHOSC_RS05860"
                     /old_locus_tag="pgaptmp_001172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706349.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MGMT family protein"
                     /protein_id="HGU4547148.1"
                     /translation="MEKEDSFPQRVWQIVAAIPEGYVTTYGDVAKLAGSPRAARQVGG
                     VLKRLPEGSTLPWHRVVNRHGTISLTGPDLQRQRQALLAEGVMVSGSGQIDLQRYRWN
                     Y"
     gene            complement(4051..4623)
                     /gene="ybaY"
                     /locus_tag="ACHOSC_RS05865"
                     /old_locus_tag="pgaptmp_001173"
     CDS             complement(4051..4623)
                     /gene="ybaY"
                     /locus_tag="ACHOSC_RS05865"
                     /old_locus_tag="pgaptmp_001173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbaY family lipoprotein"
                     /protein_id="HGU4547149.1"
                     /translation="MKLVHMASGLAVAIALAACADKSADIQTPAPAANTSISATQQPA
                     IQQPNVSGTVWIRQKVALPPDAVLTVTLSDASLADAPSKVLAQKAVRTEGKQSPFSFV
                     LPFNPADVQPNARILLSAAITVNDKLVFITDTVQPVINKGGTKADLTLVPVQQTAVPV
                     QASGGATTTVPSTSPTQVNPSSAVPAPTQY"
     gene            4841..5701
                     /gene="tesB"
                     /locus_tag="ACHOSC_RS05870"
                     /old_locus_tag="pgaptmp_001174"
     CDS             4841..5701
                     /gene="tesB"
                     /locus_tag="ACHOSC_RS05870"
                     /old_locus_tag="pgaptmp_001174"
                     /EC_number="3.1.2.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioesterase II"
                     /protein_id="HGU4547150.1"
                     /translation="MSQALKNLLTLLNLEKIEEGLFRGQSEDLGLRQVFGGQVVGQAL
                     YAAKETVPEERLVHSFHSYFLRPGDSKKPIIYDVETLRDGNSFSARRVAAIQNGKPIF
                     YMTASFQAPEAGFEHQKTMPSAPAPDGLPSETQIAQSLAHLLPPVLKDKFICDRPLEV
                     RPVEFHNPLKGHVAEPHRQVWIRANGSVPDDLRVHQYLLGYASDLNFLPVALQPHGIG
                     FLEPGIQIATIDHSMWFHRPFNLNEWLLYSVESTSASSARGFVRGEFYTQDGVLVAST
                     VQEGVMRNHN"
     gene            complement(5750..7036)
                     /gene="amtB"
                     /locus_tag="ACHOSC_RS05875"
                     /old_locus_tag="pgaptmp_001175"
     CDS             complement(5750..7036)
                     /gene="amtB"
                     /locus_tag="ACHOSC_RS05875"
                     /old_locus_tag="pgaptmp_001175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0008519 - ammonium channel activity
                     [Evidence IEA];
                     GO_process: GO:0072488 - ammonium transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ammonium transporter AmtB"
                     /protein_id="HGU4547151.1"
                     /translation="MKIATIKTGLASLAMLPGLVMAAPAVADKADNAFMMICTALVLF
                     MTIPGIALFYGGLIRGKNVLSMLTQVTVTFALVCILWVVYGYSLAFGEGNNFFGNINW
                     LMLKNIELTAVMGSIYQYIHVAFQGSFACITVGLIVGALAERIRFSAVLIFVVVWLTL
                     SYIPIAHMVWGGGLLASHGALDFAGGTVVHINAAIAGLVGAYLIGKRVGFGKEAFKPH
                     NLPMVFTGTAILYIGWFGFNAGSAGTANEIAALAFVNTVVATAAAILGWIFGEWALRG
                     KPSLLGACSGAIAGLVGVTPACGYIGVGGALIIGVVAGLAGLWGVTMLKRLLRVDDPC
                     DVFGVHGVCGIVGCIMTGIFAASSLGGVGFAEGVTMGHQLLVQLESIAITIVWSGVVA
                     FIGYKLADLTVGLRVPEEQEREGLDVNSHGENAYNA"
     gene            complement(7066..7404)
                     /gene="glnK"
                     /locus_tag="ACHOSC_RS05880"
                     /old_locus_tag="pgaptmp_001176"
     CDS             complement(7066..7404)
                     /gene="glnK"
                     /locus_tag="ACHOSC_RS05880"
                     /old_locus_tag="pgaptmp_001176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030234 - enzyme regulator activity
                     [Evidence IEA];
                     GO_process: GO:0006808 - regulation of nitrogen
                     utilization [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="P-II family nitrogen regulator"
                     /protein_id="HGU4547152.1"
                     /translation="MKLVTVIIKPFKLEDVREALSSIGIQGLTVTEVKGFGRQKGHAE
                     LYRGAEYSVNFLPKVKIDVAIADDQLDEVIDIVSKAAYTGKIGDGKIFVAELQRVIRI
                     RTGEADEAAL"
     gene            complement(7585..9366)
                     /gene="mdlB"
                     /locus_tag="ACHOSC_RS05885"
                     /old_locus_tag="pgaptmp_001177"
     CDS             complement(7585..9366)
                     /gene="mdlB"
                     /locus_tag="ACHOSC_RS05885"
                     /old_locus_tag="pgaptmp_001177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SmdB family multidrug efflux ABC transporter
                     permease/ATP-binding protein"
                     /protein_id="HGU4547153.1"
                     /translation="MRSFSQLWPTLKRLLAYGSPWRKPLGIAVLMMWVAAAAEVSGPL
                     LISYFIDNMVAKNNLPLKMVAGLAAAYVGLQLFAAGLHYAQSLLFNRAAVGVVQQLRT
                     DVMDAALRQPLSEFDTQPVGQVISRVTNDTEVIRDLYVTVVATVLRSAALVGAMLVAM
                     FSLDWRMALVAIMIFPVVLVVMVIYQRYSTPIVRRVRAYLADINDGFNEIINGMSVIQ
                     QFRQQARFGERMGEASRSHYMARMQTLRLDGFLLRPLLSLFSSLILCGLLMLFGFSAS
                     GTIEVGVLYAFISYLGRLNEPLIELTTQQAMLQQAVVAGERVFELMDGPRQQYGNDDR
                     PLQSGTIEVDNVSFAYRDDNLVLKNINLSVPSRNFVALVGHTGSGKSTLASLLMGYYP
                     LTEGEIRLDGRPLSSLSHSALRQGVAMVQQDPVVLADTFLANVTLGRDISEERVWQAL
                     ETVQLAELARSMSDGIYTPLGEQGNNLSVGQKQLLALARVLVETPQILILDEATASID
                     SGTEQAIQHALAAVREHTTLVVIAHRLSTIVDADTILVLHRGQAVEQGTHQQLLAAQG
                     RYWQMYQLQLAGEELAASVREEESLSA"
     gene            complement(9359..11131)
                     /gene="mdlA"
                     /locus_tag="ACHOSC_RS05890"
                     /old_locus_tag="pgaptmp_001178"
     CDS             complement(9359..11131)
                     /gene="mdlA"
                     /locus_tag="ACHOSC_RS05890"
                     /old_locus_tag="pgaptmp_001178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SmdA family multidrug ABC transporter
                     permease/ATP-binding protein"
                     /protein_id="HGU4547154.1"
                     /translation="MRLFAQLSWYFRREWRRYLGAVALLVIIAMLQLVPPKVVGIVVD
                     GVTEQHFTTGLILMWIATMVLIAVVVYLLRYVWRVLLFGASYQLAVELREDYYRQLSR
                     QHPEFYLRHRTGDLMARATNDVDRVVFAAGEGVLTLVDSLVMGCAVLIMMSTQISWQL
                     TLFALLPMPVMAIMIKRNGDALHERFKLAQAAFSSLNDRTQESLTSIRMIKAFGLEDR
                     QSALFAADAEDTGKKNMRVARIDARFDPTIYIAIGMANLLAIGGGSWMVVQGSLTLGQ
                     LTSFMMYLGLMIWPMLALAWMFNIVERGSAAYSRIRAMLAEAPVVNDGSEPVPEGRGE
                     LDVNIHQFTYPQTDHRALENVNFALKPGQMLGICGPTGSGKSTLLSLIQRHFDVSEGD
                     IRFHDIPLTKLQLDSWRSRLAVVSQTPFLFSDTVANNIALGCPNATQQEIEHVARLAS
                     VHDDILRLPQGYDTEVGERGVMLSGGQKQRISIARALLVNAEILILDDALSAVDGRTE
                     HQILHNLRQWGQGRTVIISAHRLSALTEASEIIVMQHGHIAQRGNHDELAQQSGWYRD
                     MYRYQQLEAALDDAPEIREEAVDA"
     gene            complement(11161..11619)
                     /gene="decR"
                     /locus_tag="ACHOSC_RS05895"
                     /old_locus_tag="pgaptmp_001179"
     CDS             complement(11161..11619)
                     /gene="decR"
                     /locus_tag="ACHOSC_RS05895"
                     /old_locus_tag="pgaptmp_001179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308528.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator DecR"
                     /protein_id="HGU4547155.1"
                     /translation="MLDKIDHKLLALLQQDCTLSLQALAEAVNLTTTPCWKRLKRLED
                     DGILIGKVALLDPEKIGLGLTAFVLIKTQHHSSEWYCRFVTVVTEMPEVLGFWRMAGE
                     YDYLMRVQVADMKRYDEFYKRLVNSVPGLSDVTSSFAMEQIKYTTSLPIE"
     gene            complement(11772..12590)
                     /gene="cof"
                     /locus_tag="ACHOSC_RS05900"
                     /old_locus_tag="pgaptmp_001180"
     CDS             complement(11772..12590)
                     /gene="cof"
                     /locus_tag="ACHOSC_RS05900"
                     /old_locus_tag="pgaptmp_001180"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414980.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016818 - hydrolase activity, acting on
                     acid anhydrides, in phosphorus-containing anhydrides
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HMP-PP phosphatase"
                     /protein_id="HGU4547156.1"
                     /translation="MARLAAFDMDGTLLMPDHHLGEKTLSTLARLRERDITLTFATGR
                     HALEMQHILGALSLDAYLITGNGTRVHSLEGELLHRDDLPADVAELVLYQQWDTRASM
                     HIFNDDGWFTGKEIPALLQAFVYSGFRYQIIDVKKMPLGRVTKICFCGGHDDLTRLQI
                     QLYEALGERAHLCFSATDCLEVLPVGCNKGAALTVLTQHLGLSLRDCMAFGDAMNDRE
                     MLGSVGSGFIMGNAMPQLRAELPHLPVIGHCRNQAVSHYLTHWLDYPHLPYSPE"
     gene            12690..14390
                     /gene="ybaE"
                     /locus_tag="ACHOSC_RS05905"
                     /old_locus_tag="pgaptmp_001181"
     CDS             12690..14390
                     /gene="ybaE"
                     /locus_tag="ACHOSC_RS05905"
                     /old_locus_tag="pgaptmp_001181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SgrR family transcriptional regulator"
                     /protein_id="HGU4547157.1"
                     /translation="MRLLNRLNQYQRLWQPSAGKPQTVTVSELAERCFCSERHVRTLL
                     RQAQEAGWLEWQAQSGRGKRGQLRFLVTPESLRNAMMEQALETGKQQDVLELAQLAPG
                     ELRTLLQPFMGGQWQNDTPTLRIPYYRPLEPLQPGFLPGRAEQHLAGQIFSGLTRFDN
                     NTQRPIGDLAHHWETSTDGLRWDFYLRSTLHWHNGDAVKASHLHQRLLMLLQLPALDQ
                     LFISVKHIEVTHPQCLTFFLHRPDYWLAHRLASYCSHLAHPQFPLIGTGPFRLTQFTA
                     ELVRLESHDYYHLRHPLLKAVEYWITPPLFEKDLGNSCRHPVQITIGKPEELQRVSQV
                     SSGISLGFCYLTLRKSPRLSLWQARKVISIIHQSGLLQTLEVGENLITASHALLPGWT
                     IPYWQVPDEVKLPKTLTLVYHLPIELHTMAERLQATLAAEGCELTIIFHNAKNWDDTT
                     LLAHADLMMGDRLIGEAPEYTLEQWLRCDPLWPHVFDAPAYAHLQSTLDAVQVMPDEE
                     NRFNALKAVFSQLMADATLTPLFNYHYRISAPPGVNGVRLTPRGWFEFTEAWLPAPSQ
                     "
     gene            14455..15150
                     /gene="queC"
                     /locus_tag="ACHOSC_RS05910"
                     /old_locus_tag="pgaptmp_001182"
     CDS             14455..15150
                     /gene="queC"
                     /locus_tag="ACHOSC_RS05910"
                     /old_locus_tag="pgaptmp_001182"
                     /EC_number="6.3.4.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="7-cyano-7-deazaguanine synthase QueC"
                     /protein_id="HGU4547158.1"
                     /translation="MKRAVVVFSGGQDSTTCLVQALQQYDEVHCVTFDYGQRHRAEID
                     VARELALKLGARAHKVLDVTLLNELAVSSLTRDSIPVPDYEPEADGIPNTFVPGRNIL
                     FLTLAAIYAYQVKAEAVITGVCETDFSGYPDCRDEFVKALNHAVSLGMAKDIRFETPL
                     MWIDKAETWALADYYGKLDLVRNETLTCYNGFKGDGCGHCAACNLRANGLNHYLADKP
                     TVMAAMKQKTGLR"
     gene            complement(15202..15600)
                     /gene="fadM"
                     /locus_tag="ACHOSC_RS05915"
                     /old_locus_tag="pgaptmp_001183"
     CDS             complement(15202..15600)
                     /gene="fadM"
                     /locus_tag="ACHOSC_RS05915"
                     /old_locus_tag="pgaptmp_001183"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long-chain acyl-CoA thioesterase FadM"
                     /protein_id="HGU4547159.1"
                     /translation="MQTQIKVRGYHLDVYQHVNNARYLEFLEEARWDGLENSDSFQWM
                     TAHNIAFVVVNININYRRPAVLSDLLTITSQLQQLNGKSGILSQVITLEPEGQVVADA
                     LITFVCIDLKTQKALALEGELREKLEQMVK"
     gene            complement(15694..16065)
                     /gene="ybaV"
                     /locus_tag="ACHOSC_RS05920"
                     /old_locus_tag="pgaptmp_001184"
     CDS             complement(15694..16065)
                     /gene="ybaV"
                     /locus_tag="ACHOSC_RS05920"
                     /old_locus_tag="pgaptmp_001184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-hairpin-helix domain-containing protein"
                     /protein_id="HGU4547160.1"
                     /translation="MKHGIKALLITLSLACAGMSHSALAAASVAKPTAVETKAEAPAA
                     QSKAAVPAKASDEEGTRVSINNASAEELARAMNGVGLKKAQAIVSYREEYGPFKTVED
                     LKQVPGMGNSLVERNLAVLTL"
     gene            complement(16216..18087)
                     /gene="ppiD"
                     /locus_tag="ACHOSC_RS05925"
                     /old_locus_tag="pgaptmp_001185"
     CDS             complement(16216..18087)
                     /gene="ppiD"
                     /locus_tag="ACHOSC_RS05925"
                     /old_locus_tag="pgaptmp_001185"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HGU4547161.1"
                     /translation="MMDSLRTAANSLVLKIIFGIIIVSFILTGVSGYLIGGGNNYAAK
                     VNDQEISRGQFENAFNSERNRMQQQLGDQYSELAANEGYMKTLRQQVLNRLIDEALLD
                     QYARELKLGISDEQVKQAIFATPAFQVDGKFDNSRYNGILNQMGMTADQYAQALRNQL
                     TTQQLINGVAGTDFMLKGETDELAALVAQQRVVREATIDVNALAAKQPVTEQEIASYY
                     EQNKNNFMTPEQFRVSYIKLDAATMQQPVSDADIQSYYDQHQDQFTQPQRTRYSIIQT
                     KTEDEAKVVLDELNKGGDFAALAKEKSADIISARNGGDMGWLEDATIPDELKNAGLKE
                     KGQLSGVIKSSVGFLIVRLDDIQPAKVKSLDEVRDDIAAKVKHEKALDAYYALQQKVS
                     DAASNDTESLAGAEQAAGVKATQTGWFSKDNLPEELNFKPVADAIFNGGLVGENGAPG
                     INSDIITVDGDRAFVLRISEHKPEAVKPLADVQEQVKALVQHNKAEQQAKVDAEKLLV
                     DLKAGKGAEAMQAAGLKFGEPKILSRSGRDPISQAAFALPLPAKDKPSYGMATDMQGN
                     VVLLALDEVKQGSMPEDQKKAMVQGITQNNAQIVFEALMSNLRKEAKIKIGDALEQQ"
     gene            complement(18279..18569)
                     /gene="hupB"
                     /locus_tag="ACHOSC_RS05930"
                     /old_locus_tag="pgaptmp_001186"
     CDS             complement(18279..18569)
                     /gene="hupB"
                     /locus_tag="ACHOSC_RS05930"
                     /old_locus_tag="pgaptmp_001186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013356995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoid-associated protein HU-beta"
                     /protein_id="HGU4547162.1"
                     /translation="MKRKRRVNKSQLIDKIAAGADISKAAAGRALDAIIASVTESLKE
                     GDDVALVGFGTFAVKERAARTGRNPQTGKEITIAAAKVPSFRAGKALKDAVN"
     gene            complement(18760..21114)
                     /gene="lon"
                     /locus_tag="ACHOSC_RS05935"
                     /old_locus_tag="pgaptmp_001187"
     CDS             complement(18760..21114)
                     /gene="lon"
                     /locus_tag="ACHOSC_RS05935"
                     /old_locus_tag="pgaptmp_001187"
                     /EC_number="3.4.21.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001469947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004176 - ATP-dependent peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endopeptidase La"
                     /protein_id="HGU4547163.1"
                     /translation="MNPERSERIEIPVLPLRDVVVYPHMVIPLFVGREKSIRCLEAAM
                     DHDKKIMLVAQKEASTDEPGVNDLFTVGTVASILQMLKLPDGTVKVLVEGLQRARISA
                     LSDNGEHFSAKAEYLESPTIDEREQEVLVRTAISQFEGYIKLNKKIPPEVLTSLNSID
                     DPARLADTIAAHMPLKLADKQSVLEMSDVNERLEYLMAMMESEIDLLQVEKRIRNRVK
                     KQMEKSQREYYLNEQMKAIQKELGEMDDAPDENEALKRKIDAAKMPKEAKEKAEAELQ
                     KLKMMSPMSAEATVVRGYIDWMVQVPWNARSKVKKDLRQAQEILDTDHYGLERVKDRI
                     LEYLAVQSRVNKIKGPILCLVGPPGVGKTSLGQSIAKATGRKYVRMALGGVRDEAEIR
                     GHRRTYIGSMPGKLIQKMAKVGVKNPLFLLDEIDKMSSDMRGDPASALLEVLDPEQNV
                     AFSDHYLEVDYDLSDVMFVATSNSMNIPAPLLDRMEVIRLSGYTEDEKLNIAKRHLLP
                     KQIERNALKKGELTVDDSAIIGIIRYYTREAGVRGLEREISKLCRKAVKQLLLDKSLK
                     HIEINGDNLHDYLGVQRFDYGRADNENRVGQVTGLAWTEVGGDLLTIETACVPGKGKL
                     TYTGSLGEVMQESIQAALTVVRARAEKLGINPDFYEKRDIHVHVPEGATPKDGPSAGI
                     AMCTALVSCLTGNPVRADVAMTGEITLRGQVLPIGGLKEKLLAAHRGGIKTVLIPFEN
                     KRDLEEIPDNVIADLDIHPVKRIEEVLTLALQNEPSGMQVVTAK"
     gene            complement(21302..22576)
                     /gene="clpX"
                     /locus_tag="ACHOSC_RS05940"
                     /old_locus_tag="pgaptmp_001188"
     CDS             complement(21302..22576)
                     /gene="clpX"
                     /locus_tag="ACHOSC_RS05940"
                     /old_locus_tag="pgaptmp_001188"
                     /EC_number="3.4.21.92"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001396736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009368 - endopeptidase Clp complex
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_function: GO:0140662 - ATP-dependent protein folding
                     chaperone [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent protease ATP-binding subunit ClpX"
                     /protein_id="HGU4547164.1"
                     /translation="MTDKRKDGSGKLLYCSFCGKSQHEVRKLIAGPSVYICDECVDLC
                     NDIIREEIKEVAPHRERSALPTPHEIRNHLDDYVIGQEQAKKVLAVAVYNHYKRLRNG
                     DTSNGVELGKSNILLIGPTGSGKTLLAETLARLLDVPFTMADATTLTEAGYVGEDVEN
                     IIQKLLQKCDYDVQKAQRGIVYIDEIDKISRKSDNPSITRDVSGEGVQQALLKLIEGT
                     VAAVPPQGGRKHPQQEFLQVDTSKILFICGGAFAGLDKVISHRVETGSGIGFGATVKA
                     KSDKASEGELLAQVEPEDLIKFGLIPEFIGRLPVVATLNELSEEALIQILKEPKNALT
                     KQYQALFNLEGVDLEFRDEALDAIAKKAMARKTGARGLRSIVEAALLDTMYDLPSMED
                     VEKVVIDESVIDGQSKPLLIYGKPEAQQASGE"
     gene            complement(22702..23325)
                     /gene="clpP"
                     /locus_tag="ACHOSC_RS05945"
                     /old_locus_tag="pgaptmp_001189"
     CDS             complement(22702..23325)
                     /gene="clpP"
                     /locus_tag="ACHOSC_RS05945"
                     /old_locus_tag="pgaptmp_001189"
                     /EC_number="3.4.21.92"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006809880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009368 - endopeptidase Clp complex
                     [Evidence IEA];
                     GO_function: GO:0004176 - ATP-dependent peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp endopeptidase proteolytic
                     subunit ClpP"
                     /protein_id="HGU4547165.1"
                     /translation="MSYSGERDNFAPHMALVPMVIEQTSRGERSFDIYSRLLKERVIF
                     LTGQVEDHMANLIVAQMLFLEAENPEKDIYLYINSPGGVITAGMSIYDTMQFIKPDVS
                     TICMGQAASMGAFLLTAGAKGKRFCLPNSRVMIHQPLGGYQGQATDIEIHAREILKVK
                     GRMNELMALHTGQSLEQIERDTERDRFLSAPEAVEYGLVDSILTHRN"
     gene            complement(23572..24870)
                     /gene="tig"
                     /locus_tag="ACHOSC_RS05950"
                     /old_locus_tag="pgaptmp_001190"
     CDS             complement(23572..24870)
                     /gene="tig"
                     /locus_tag="ACHOSC_RS05950"
                     /old_locus_tag="pgaptmp_001190"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001716227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005854 - nascent polypeptide-associated
                     complex [Evidence IEA];
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0015031 - protein transport [Evidence IEA];
                     GO_process: GO:0051083 - 'de novo' cotranslational protein
                     folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trigger factor"
                     /protein_id="HGU4547166.1"
                     /translation="MQVSVETTQGLGRRVTITIAADSIETAVKSELVNVAKKVRIDGF
                     RKGKVPMNIVAQRYGASVRQDVLGDLMSRNFIDAIIKEKINPAGAPTYVPGEYKLGED
                     FTYSVEFEVYPEVELQGLEAIEVEKPIVEVTDADVDGMLDTLRKQQATWKEKDGAVEA
                     EDRVTIDFTGSVDGEEFEGGKASDFVLAMGQGRMIPGFEDGIKGHKAGEEFTIDVTFP
                     EEYHAENLKGKAAKFAINLKKVEERELPELTAEFIKRFGVEDGSVEGLRAEVRKNMER
                     ELKSAIRNRVKSQAIEGLVKANDIDVPAALIDSEIDVLRRQAAQRFGGNEKQALELPR
                     ELFEEQAKRRVVVGLLLGEVIRTNELKADEERVKGLIEEMASAYEDPKEVIEFYSKNK
                     ELMDNMRNVALEEQAVEAVLAKAKVTEKETTFNELMNQQA"
     gene            complement(25214..25531)
                     /gene="bolA"
                     /locus_tag="ACHOSC_RS05955"
                     /old_locus_tag="pgaptmp_001191"
     CDS             complement(25214..25531)
                     /gene="bolA"
                     /locus_tag="ACHOSC_RS05955"
                     /old_locus_tag="pgaptmp_001191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414969.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator BolA"
                     /protein_id="HGU4547167.1"
                     /translation="MMIRERIEEKLRAAFQPVFLEVVDESYRHNVPAGSESHFKVVLV
                     SDRFTGERFLNRHRMIYSTLAEELSTTVHALALHTYTIKEWEGLQDTVFASPPCRGAG
                     SIA"
     gene            25836..26414
                     /gene="yajG"
                     /locus_tag="ACHOSC_RS05960"
                     /old_locus_tag="pgaptmp_001192"
     CDS             25836..26414
                     /gene="yajG"
                     /locus_tag="ACHOSC_RS05960"
                     /old_locus_tag="pgaptmp_001192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414968.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HGU4547168.1"
                     /translation="MFKKILFPLVALFMLAGCAKPPTTIEVSPTITLPQQDPSLMGVT
                     VSINGADQRTDQALAKVTRDNQIVTLTASRDLRFLLQEVLEKQMTARGYMVGPNGPVN
                     LQIIVSQLYADVSQGNVRYNIATKADIAIIATAQNGNKMTKNYRASYNVEGAFQASNK
                     NIADAVNSVLTDTIADMSQDTSIHEFIKQNAR"
     gene            26458..27933
                     /gene="ampG"
                     /locus_tag="ACHOSC_RS05965"
                     /old_locus_tag="pgaptmp_001193"
     CDS             26458..27933
                     /gene="ampG"
                     /locus_tag="ACHOSC_RS05965"
                     /old_locus_tag="pgaptmp_001193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="muropeptide MFS transporter AmpG"
                     /protein_id="HGU4547169.1"
                     /translation="MSSQYLRIFQQPRSAILLILGFASGLPLALTSGTLQAWMTVENI
                     DLKTIGFFSLVGQAYVFKFLWSPLMDRYTPPFFGRRRGWLLATQILLLVAIAAMGFLE
                     PGTQLRWMAALAVVIAFCSASQDIVFDAWKTDVLPAEERGAGAAISVLGYRLGMLVSG
                     GLALWLADKWLGWQGMYWLMAALLIPCIIATLLAPEPTDTIPVPKTLEQAVVAPLRDF
                     FGRNNAWLILLLIVLYKLGDAFAMSLTTTFLIRGVGFDAGEVGVVNKTLGLLATIVGA
                     LYGGILMQRLSLFRALLIFGILQGASNAGYWLLSITDKHLYSMGAAVFFENLCGGMGT
                     SAFVALLMTLCNKSFSATQFALLSALSAVGRVYVGPVAGWFVEAHGWSTFYLFSVAAA
                     VPGLILLLVCRQTLEYTGVNDNFISRTEYPAGYAFAMWTLAAGVSLLAVWLLLLTMDA
                     LDLTHFSFLPALLEVGVLVALSGVVLGGLLDYLALRKTHLT"
BASE COUNT         6625 a         7637 c         6773 g         6976 t
ORIGIN      
        1 atcacacaga tgtaatagga acgttctctt cactgacttt tcgtcttact gtgttgccgc
       61 attttcagca accggaggtc agtaatgagc acagaaaaag aaaagatgat tgctggtgag
      121 ttgtatcgct cggcagatga gacgttatct cgcgatcgcc tgcgcgctcg tcagcttatt
      181 caccgataca atcattccct ggcggaagag cacacattac gccagcaaat tctcgctgat
      241 ctattcggtc aggtgacaga ggcttatatt gagccaacgt ttcgctgtga ctatggctat
      301 aacatttttc tcggtaataa ttttttcgcc aacttcgatt gcgtgatgct tgatgtctgc
      361 cctattcgca tcggtgataa ctgtatgttg gcaccaggcg ttcatatcta cacggcaaca
      421 catcccatcg accctgtagc acgtaatagc ggtgctgaac tggggaaacc cgtcaccatc
      481 ggtaataacg tctggattgg cggacgcgcg gtcattaacc ctggtgtgac cattggtgat
      541 aacgtcgtgg tagcctcagg tgcagttgtc acaaaagatg tcccggacaa cgttgtcgtg
      601 ggcggtaatc cagccagaat aattaaaaaa ttgtaatcgg tttttcgcaa ctgttatgca
      661 aaattgtggt agatctgtta cttcccctct actattccca cgttaaaata gggtgttccc
      721 tggaaagttg cagataccac gaaggcaaac gatgaccgaa atacaacgcc tgctgaccga
      781 aacgattgag tctctgaata cccgcgaaaa acgcgacaac aaaccccgct ttagtatcag
      841 ttttatccgt aaacatccgg ggctgtttat cggtatgtac gttgcttttt ttgccaccct
      901 ggcggtgatg ttgcagtccg aaacgctgtc aggctctgtc tggctactgg ttgtattatt
      961 tatcctgctt aatggtttct tctttttcga tgtctaccca cgctaccgct atgaagatat
     1021 cgacgtgctg gatttccgcg tttgctataa cggcgaatgg tacaacacgc gctttgtacc
     1081 tgccgcgctg gttgaagcca tcttgaactc tccgcgtgtc gcggatgttc ataaggaaca
     1141 actgcaaaaa atgatcgtcc gtaaaggtga actgtctttt tacgatattt ttaccctcgc
     1201 tcgcgccgaa tcaacatctt aagttagggt tacataccag gcgtaaagct ctgcgcctgg
     1261 tcaaatgaca atgatcgttt ccacccatca cttcatgaaa taccagctct acctccttat
     1321 ctcctgccag cctttttcca caatcagata tactttccct acactgtgtt aataaggata
     1381 tgctggtgag aacacgacat ctggtcggcc ttatttcggg agtactgatt ctttcagtat
     1441 tgctgcctgt cggcttaagc atctggctgg cccatcagca ggtagaaaca tcgtttattg
     1501 aagagctgga tacctattcc tcccgcgtcg ctattcgggc caataaggtg gcgacacaag
     1561 ggaaagatgc gctgcaagag ctggaaagat ggcaaggcgc tgcctgtagc gaagcccatc
     1621 tcatggaaat gcgtcgggta tcttacagtt atcgctatat tcaggaagtg gtttatatcg
     1681 ataacaacgt tccccagtgt tcgtctctgg agcatgaaag tccgcccgat accttccccg
     1741 agccaggtaa aatttcgaaa gatggttatc gtgtctggtt aacatcgcat aacgatttag
     1801 gcattatccg ttacatggtc gccatgggaa cggcacatta tgtcgtcatg atcgaccccg
     1861 cttcctttat tgatgtcatt ccctatagct catggcaaat tgatgccgcc attattggca
     1921 atgcccataa cgttgtcata accagcagcg atgaaattgc tcagggaatt attaccaggc
     1981 tacaaaaaac acccggtgag catatcgaaa ataatggaat catttacgat atcctgccct
     2041 tcccggagat gaatatttcg atcatcacct gggcttcaac gaaaatgttg cagaaaggct
     2101 ggcatcggca agtctttatt tggttaccgc tcgggttggt gattggccag ctggcagcga
     2161 tgtttgtgct gcgtattttg cgccgtatcc agtcaccgca tcatcggctg caggatgcta
     2221 tcgaaaatcg tgatatttgc gtgcactatc agccgattgt ctccttagcc aatggcaaaa
     2281 ttgtcggtgc tgaggcactg gcgcgctggc cgcagacaga cggtagttgg ttgtcaccag
     2341 atagttttat tccgctggca cagcaaacgg gcctttctga gccattgacg ctactgatta
     2401 taagaagcgt ctttgaagat atgggcgact ggctgcgtca gcatccacag cagcatattt
     2461 cgatcaatct tgaatccacc gtgctcacct cggaaaaaat cccgcaattg ctgcgtgaaa
     2521 tgatcaatca ctatcaggtt aatcccagac agatcgcgct tgaactcact gaacgcgagt
     2581 ttgccgatcc gaaaaccagc gccccgataa tttctcgcta ccgggaggcg ggccatgaaa
     2641 tttatcttga tgattttggt acgggatatt caagtttaag ttatttacag gatctggatg
     2701 tcgacattct gaagatcgat aaatctttcg ttgatgcgct ggaatataaa aatgtcacgc
     2761 cgcatatcat cgaaatggca aaaacactga aactgaaaat ggtagcggag ggaatcgaaa
     2821 ccagtaaaca agaagagtgg ctacgccagc atggcgtgca ctacggtcag ggctggctct
     2881 acagcaaggc attaccgaaa gaagatttct tacgctgggc cgagcaacat ttgtgaattt
     2941 ttgggctgac ggcacgccgc acagcccgtc acgctaccta ttcgtcgatg attgactcga
     3001 atccgccata gatcattcgc ttgccgtcaa acggcatgga ctcgccgaac tctttcatcc
     3061 gtgggtccga catcatcttt tgattagcag cgtcgcggac ctcttttgaa gggtattcaa
     3121 tccagctaaa gacaacctct tcattctctt ccgctttcac cgccatacga aaatcggtca
     3181 ctttgccatc cggtacatcg ctggcccagc attcgacaat acgaagcgcg ccaaactctt
     3241 taaacaatgg cgcagcctta gcggccattt cccgataggc atccttttta tcggcaggaa
     3301 cggcaaccac aaaaccatca acatacttca tcagacatac ctccgaagcg tactgcgcag
     3361 ccagacaatt cttgcgacgt cagttgtggt taaagtttag ccatgttggt tttttttgac
     3421 gaaacggtgg gaggcagaaa tgggtggggg ccctgccagc tacatcccgg cacacgcgtc
     3481 atctgccttg gctgcttcct tccggacctg acctggtaaa cagagtagcg ttgcgggaga
     3541 accaacaggc ccccattgag agcgttgaga accaacgcgc aggcgcatta tcactgctgc
     3601 cccggctaat tgcaacccat cgcgtgccag atgctggttt cttgcgcaat gcgacttcac
     3661 tcgggcgttt ttccagatta tgctgaaaaa ttacctcagg aagaaaagat ggaaaaagaa
     3721 gattcatttc cccaacgcgt ctggcaaatc gtcgccgcta ttcccgaagg ctatgtcacc
     3781 acttacggtg atgtggcgaa actggcggga tcgccccgcg ccgcgcgcca ggtgggcggt
     3841 gtgttaaagc gtctccctga aggcagcacc ttaccctggc accgggtggt taatcgccac
     3901 ggcacaattt cgctaaccgg accggattta cagcgtcagc gacaggcatt actggcagaa
     3961 ggtgtgatgg tatcgggaag cgggcaaatc gacttgcagc gttatcgctg gaactactaa
     4021 ccctctccgg gcggagaggg ttaacgtcgc ttaatattgc gtaggagctg gaactgccga
     4081 agacggattc acctgagttg gtgaagtcga aggtacggta gtcgttgcac caccgctggc
     4141 ctgaaccggc acggcggttt gctgcaccgg caccaatgtc aggtcggctt tagttccgcc
     4201 cttgttgatc accggctgaa cggtatcggt aataaatacc agtttgtcat tcacggtaat
     4261 cgccgcactc aacagaatac gcgcgttcgg ctgaacatct gccggattaa atggcagaac
     4321 aaagctgaat ggtgactgtt taccttcggt acgcaccgct ttctgcgcca gcacttttga
     4381 cggtgcatcg gctaacgacg cgtcagaaag tgtcacggtc agcacagcat caggcggcag
     4441 tgcgactttc tgccggatcc agacggtacc ggagacattc ggttgctgga tagcaggttg
     4501 ttgtgttgct gaaatagatg tatttgcagc cggggctggc gtctgaatat ccgcgctttt
     4561 atctgcgcaa gccgccaacg caatcgcaac cgctaaacca ctggccatgt gcacgagttt
     4621 cattcatttc tccttattat caatgcacca gcgggctaac tttcctcgcc ggaagagtgg
     4681 ttaacaaaat agtaacgtca acaagtgtgg cacacatcac gcatttctgc ctgtaattag
     4741 cccgtaattc agaccattgc acccatcgga ccagtagcaa atttgcgtta tactcaactc
     4801 actttggctt gctgcggcag ctttgttact ggagagttat atgagtcagg cgctaaaaaa
     4861 tttactgaca ttgttaaatc tggaaaaaat tgaggaagga ctctttcgcg gccagagtga
     4921 agatttaggt ttacgccagg tgtttggcgg ccaggtcgtg ggtcaggcct tgtatgctgc
     4981 aaaagagacc gtccctgaag agcggctggt acattcgttt cacagctact ttcttcgccc
     5041 tggcgatagt aagaagccga ttatttatga tgtcgaaacg ctgcgtgacg gtaacagctt
     5101 cagcgcccgc cgggttgctg ctattcaaaa cggcaaaccg attttttata tgacggcctc
     5161 tttccaggca ccagaagcgg gtttcgaaca tcaaaaaaca atgccgtccg cgccagcgcc
     5221 tgatggcctg ccttcggaaa cgcaaatcgc ccaatcgctg gcgcacctgc tgccgccagt
     5281 gcttaaagat aaattcatct gcgatcgtcc gctggaagtc cgtccggtgg agtttcataa
     5341 cccactgaaa ggtcacgtcg cagaaccaca tcgtcaggtg tggatccgcg caaatggtag
     5401 cgtgccggat gacctgcgcg ttcatcagta tctgctcggt tacgcttctg atcttaactt
     5461 cctgccggta gctctacagc cgcacggcat cggttttctc gaaccgggga ttcagattgc
     5521 caccattgac cattccatgt ggttccatcg cccgtttaat ttgaatgaat ggctgctgta
     5581 tagcgtggag agcacctcgg cgtccagcgc acgcggcttt gtgcgcggtg agttttatac
     5641 ccaggacggc gtgctggttg cctcgaccgt tcaggaaggg gtgatgcgta atcacaatta
     5701 aaaaaaatag ccggaggtaa aaccgtccgg ctattttttg cagtgcttgt tacgcgttat
     5761 aggcattctc gccgtgactg ttgacatcca gcccttctcg ctcctgctct tccggtacac
     5821 gcagaccaac cgtcagatcc gccagtttgt agccaataaa tgccaccaca ccggaccaga
     5881 cgatcgtaat ggcgatgctt tccagctgta ccagcaactg atggcccatc gtcacacctt
     5941 cagcgaagcc cacgccaccc agcgaactgg cggcaaaaat cccagtcatg atacagccga
     6001 caatgccaca aacgccgtgc acaccgaaga catcgcaggg atcatccacc cgcagcaagc
     6061 gtttgagcat agtaacgccc cacaagcccg ccagaccagc taccacgccg ataatcaacg
     6121 cgccgccaac cccaatgtag ccacacgctg gcgtcacgcc gaccagaccg gcaatcgcgc
     6181 cagaacacgc tcccagcaga gaaggcttac cacgcagcgc ccattcaccg aagatccagc
     6241 caagaattgc cgccgccgtt gcgaccacag tattcacaaa tgccagtgcc gcgatttcat
     6301 tcgccgtgcc cgctgacccg gcgttaaagc caaaccaacc aatatagaga atggcagtac
     6361 cggtgaagac cattggcagg ttgtgcggtt taaacgcctc tttgccgaag cccacgcgtt
     6421 tgcctatcag atacgcgccc accagaccgg cgattgcggc gttaatatgc accaccgtgc
     6481 cacccgcgaa atccagcgca ccgtgagaag ccagcaaacc accgccccac accatatgcg
     6541 caatcggaat gtaagagagc gtcagccata ccaccacgaa aatcaacaca gctgagaagc
     6601 ggattcgttc cgccagcgcc ccaacgatca agccgacggt aatgcaggca aacgagccct
     6661 gaaacgccac gtggatatac tgataaatgc tgcccatcac cgccgtcagt tcgatgtttt
     6721 ttagcatcaa ccagttaatg ttgccgaaga agttgttgcc ctcaccaaac gccagcgagt
     6781 aaccgtaaac cacccagaga atacagacca gtgcaaacgt caccgtcacc tgcgtcagca
     6841 tcgacagcac gtttttgccg cgaatcaacc caccgtaaaa cagggcaatc cccggaatag
     6901 tcataaacag caccagcgca gtacaaatca tcataaacgc attgtcggct ttatcggcca
     6961 ccgcaggtgc agccattacc agtcccggca gcatcgccag tgaagcaagc ccagttttta
     7021 tcgtcgctat cttcattttt cgttcctgtt gctgtgtgcc agagattaca gcgccgcttc
     7081 gtcggcttcg ccggtacgaa tacgaatgac gcgttgcaat tcagcgacga agattttgcc
     7141 gtcgccaatt tttccggtgt aagccgcctt actgacgata tcgatcactt catcgagttg
     7201 gtcatcagca atcgccacat caatttttac ttttggcagg aagttgacgc tgtattccgc
     7261 cccccggtac agctcggcat gccctttctg acgcccgaaa cctttcactt cggtgacggt
     7321 caggccctga ataccaatgg aagataacgc ttcacgaacg tcttccagct tgaatggttt
     7381 gattatcacg gtcaccagct tcatagatcc cctccggtca gaattcggta atggttctgc
     7441 tacacgaaga aggtattgca agcggtgtgc cagaaatgaa aaacgagaaa gagagcagga
     7501 agttaagaag attgaaaatg aaaacgcact atgacagtgc acagtgcgtt acatttttgc
     7561 accaacgatg aaatattgcg ctattcaggc gctcaatgat tcttcttcac gcacgctggc
     7621 tgccagctct tcgcccgcaa gttgcagttg atacatctgc cagtagcgtc cctgggccgc
     7681 cagcagttgc tggtgagtgc cctgctccac ggcttgccca cgatgaagca ccagaatggt
     7741 gtcggcatca acaatggtcg ataagcggtg agcaatcacc accagcgtgg tatgttcacg
     7801 cacagccgcc agagcatgtt gaatcgcctg ttcagtaccg gagtcaatgc tggcggttgc
     7861 ctcatcaagg atcaggattt gcggcgtctc caccaacacg cgtgccagtg ccagcagttg
     7921 cttttgcccg acggagagat tattcccctg ctcgcccagc ggcgtataaa taccatcact
     7981 catgctgcgc gccagctccg ccagttgcac ggtttccagc gcctgccaga cgcgttcttc
     8041 ggagatatca cgccccagcg tcacgttggc gaggaaggta tccgccagca ccaccggatc
     8101 ttgctgcacc attgccacgc cctggcgcag cgcgctgtga cttagcgaac ttaatggacg
     8161 accatcaagg cgaatctcac cttccgttag cgggtaatag cccatcaata aactggcgag
     8221 ggtgcttttg ccactgccgg tatgcccgac cagtgccaca aagttacgcg aaggcacaga
     8281 gagattaatg ttctttagca ccagattgtc atcgcgataa gcaaatgaca cgttatcgac
     8341 ttcgatggtg ccactctgta acgggcgatc atcattgcca tattgctggc gcggtccgtc
     8401 catcagttca aacacgcgct caccagcaac aacagcctgt tgcagcatcg cctgttgcgt
     8461 ggtcagttcg attaatggtt cgttaagtcg cccaagatag ctgataaacg catacagcac
     8521 gcccacttca atggtgccgc tagcggagaa gccaaacagc atcaacaagc cacaaagaat
     8581 gagcgatgaa aacagactca gcagcggacg cagcagaaaa ccgtcgaggc gcagggtttg
     8641 catcctggcc atatagtgtg aacgactggc ctcccccata cgttcgccaa atcgcgcctg
     8701 ctgacggaac tgctggataa cgctcatgcc attgatgatt tcgttaaagc cgtcgttgat
     8761 atccgctaga taggcgcgca cacgacggac aatcggcgtg ctgtaacgct ggtatatcac
     8821 cattaccacc agcaccaccg ggaaaatcat tatcgccacc agcgccattc gccagtcgag
     8881 gctgaacatc gccaccagca tcgcgcccac cagtgcggcg ctacgcagga cagttgccac
     8941 tacggtaacg tagagatcgc ggatcacttc agtgtcatta gtgacgcggg aaatcacctg
     9001 cccgacgggt tgggtatcaa actcgcttaa tggctggcgc aatgcggcat ccatcacgtc
     9061 ggtacgcaac tgttgcacta cgcctactgc cgcccgatta aacagcagcg actgcgcgta
     9121 atgtagcccg gcggcaaaca gttgtagccc aacatacgcc gcagccagcc ctgcaaccat
     9181 tttcaacggc aggttatttt tcgctaccat attgtcgata aaatagctga taagcagcgg
     9241 cccactgact tctgccgctg ccgcaaccca catcatcagg accgcaatcc ccagcggttt
     9301 acgccacggc gaaccgtacg ctaacagacg cttgagagtc ggccacagtt ggctaaaact
     9361 acgcatcgac ggcctcctcg cgaatttccg gagcgtcgtc gagcgccgcc tccagttgtt
     9421 gatagcgata catatcgcga taccagccgc tttgttgcgc cagctcatca tgattgccac
     9481 gctgggcgat atgtccgtgc tgcatcacaa taatttcact ggcttccgtc agtgccgaaa
     9541 ggcgatgggc actgatgatt accgttctac cctgtcccca ctgacgcagg ttatgcagga
     9601 tctggtgctc tgtgcgtccg tccaccgccg aaagcgcatc atcaaggatg aggatttccg
     9661 cgttgactaa taacgcacga gcaatagaga tacgctgttt ttgcccgccg gaaagcatca
     9721 cgccgcgctc gcccacctct gtatcgtaac cttgcggtaa acgcaaaata tcgtcatgta
     9781 cgctggctaa ccgcgcgaca tgctcaatct cttgctgggt ggcattcggg caacccaacg
     9841 cgatgttgtt cgctacggtg tcggaaaaaa ggaacggtgt ctggctaact accgccaggc
     9901 ggctacgcca gctatcgagt tgtaacttcg tcagaggaat atcatgaaag cgaatatccc
     9961 cctcgctgac gtcgaaatga cgctgaatga gcgacaacag ggtacttttg ccggaaccag
    10021 tcggcccgca gatacccagc atctgaccgg gtttcagggc gaaattgacg ttttccagcg
    10081 cacgatggtc agtctgcgga tacgtgaact ggtgaatatt tacatcaagt tcgccacgcc
    10141 cttccggcac gggttcgcta ccatcgttca ccaccggcgc ttccgccagc atcgcacgaa
    10201 tacggctgta cgcagcacta ccacgttcca caatgttaaa catccatgcc agcgccagca
    10261 ttggccaaat catcagacct aaatacatca taaaactggt gagctggccc agcgttaaac
    10321 tgccctgcac caccatccaa ctaccgccgc caatcgccag caagttcgcc ataccaatcg
    10381 cgatatagat tgtcgggtcg aaacgagcat caatacgcgc cacccgcatg ttttttttgc
    10441 cggtatcttc ggcatccgcg gcaaataacg ccgactggcg atcttccaga ccaaaggctt
    10501 tgatcatgcg gatactggtg aggctttcct gggtgcggtc attaagactg gaaaacgccg
    10561 cctgtgccag cttaaagcgt tcatgcaagg catcgccgtt gcgcttaatc atgatcgcca
    10621 tcactggcat cggtaacagg gcaaataagg tcaactgcca gctaatttgc gtagacatca
    10681 taatcaacac agcgcagccc attaccagtg aatccaccag cgtcagcacc ccttctccgg
    10741 cggcaaacac gacgcgatcg acgtcattgg tcgcacgagc catgagatca cccgtgcgat
    10801 gacgcaggta aaactccggg tgctgccggc ttagctgacg gtaataatct tcacgcagtt
    10861 caacagccag ttggtaagac gcgccaaaca gcaatacgcg ccagacataa cgcaggagat
    10921 aaaccacaac ggcaatcagc accatggtgg cgatccacat caggatcagc ccggtagtaa
    10981 agtgttgttc tgtcacgcca tcgacaacaa taccaaccac ttttggcgga accagttgca
    11041 gcatcgcgat aatgacaagc aaggcgacag ccccgagata gcgacgccat tcccgacgga
    11101 aataccagct taattgagca aataatcgca cgcgttgtgt cctgacctga ttctggatat
    11161 ttattcgatg ggtaaagaag tggtgtattt aatctgttcc atcgcgaagc tggaagtgac
    11221 gtccgacagc cccggcacgc tgtttaccag acgcttataa aactcgtcgt agcgtttcat
    11281 gtcggcaacc tggacgcgca tcagataatc gtattcacca gccatgcgcc agaaccccag
    11341 cacttctggc atttcggtaa ccaccgtgac aaagcggcaa taccattcgc tgctgtgatg
    11401 ttgcgttttt atcagcacaa aagcggtcag gccgaggcct attttttccg gatccagcag
    11461 ggcgactttg ccgataagga taccgtcgtc ctccagccgt ttcagacgct tccagcaagg
    11521 ggtggttgtc agattaacgg cttcagccag tgcctgcaaa gagagggtgc aatcctgctg
    11581 cagtaaggcc agcagcttat ggtcaatttt atctaacata gcccttccac agagaatttt
    11641 tttctctttt aattctattt taaaggtgaa atcacgacaa tttattctgt gcggtttaaa
    11701 caccacggca caaaatgaca aattcaacgc aaaaaaaacc gggcaattgc ccggtgctgg
    11761 aagggatctc gttattcggg ggaataaggt agatgtggat agtccagcca gtgcgtcaaa
    11821 tagtgagaga cagcctgatt tcggcaatgt ccaatcaccg gtaaatgcgg gagctccgcg
    11881 cgcagttgcg gcatcgcatt gcccataata aacccgctac cgacgctgcc taacatttcg
    11941 cgatcgttca tcgcatcacc aaaggccatg caatcgcgca acgataaacc taaatgttgg
    12001 gtcagcaccg tcaatgcagc gcctttattg cagcccaccg gcagcacttc gaggcaatcc
    12061 gtggcggaaa aacacaaatg tgcacgctcg cctaatgctt cgtatagctg gatctgcaag
    12121 cgtgtaagat cgtcgtgacc gccacagaag cagatcttgg tgactctgcc gagtggcatt
    12181 tttttgacat cgattatctg ataacgaaaa ccgctataga caaatgcctg caacaacgca
    12241 gggatctctt tcccggtaaa ccaaccgtcg tcattgaaga tatgcatgct ggctcgggta
    12301 tcccattgct gatacagcac cagctccgcg acatccgcag gtaaatcatc acgatgtaaa
    12361 agttcacctt ccagagaatg cacgcgcgtt ccgttgccgg taatcaaata tgcatccagc
    12421 gatagcgccc cgagaatatg ctgcatctcc agcgcatgac gccccgtggc aaaagtgagg
    12481 gtaatgtcgc gttcacgcag tcgcgccaaa gtagagaggg ttttctcacc taaatgatgg
    12541 tcaggcatca ataaagtgcc atccatatca aatgctgcca gacgagccat ttctttctcc
    12601 actctgtgac acggttaatg gtgattgagt atcacctgat atatacggaa gtaatagtga
    12661 atagttaaat aatattgttc cgggtttata tgcgattgct caaccgtctt aaccagtatc
    12721 aacgtctgtg gcaaccttca gccggaaagc cgcaaaccgt caccgtcagc gaactggccg
    12781 aacgctgttt ttgcagcgaa cgccatgttc gtacgctgtt gcgtcaggca caggaggcgg
    12841 gatggctgga gtggcaggcg cagtcaggac gcggaaagcg cggacaatta cgctttctgg
    12901 tcacgccgga atcgctacgc aatgcgatga tggaacaggc actggaaacc ggaaagcagc
    12961 aagatgtgct ggagctggcg caactggccc caggtgagct gcgcactctg ttacagccgt
    13021 ttatgggcgg acaatggcaa aacgatacac ccacgttgcg tattccctac tatcgcccgc
    13081 tcgaaccgct acaaccaggc tttttgcccg gccgtgccga gcagcatctc gccgggcaga
    13141 tattttccgg cctgacccgc ttcgataata atactcagcg cccgattggc gatttagcgc
    13201 atcactggga aacctctact gacgggttac gctgggactt ttatcttcgt tcaaccctac
    13261 actggcataa cggcgatgca gtaaaagcct cacacttaca ccagcgatta ttgatgctgt
    13321 tacaactgcc agcactggat caattattta ttagcgtgaa gcatattgaa gtcacccatc
    13381 cgcagtgtct gaccttcttt ttacatcgcc ccgattactg gcttgcgcac cggctggcga
    13441 gctattgcag ccatctggcg catccgcaat tcccactgat cggcactggt ccttttcgct
    13501 taacacaatt cacagcggag ctggtgcgcc tggagagcca tgattattac catttgcgtc
    13561 atccgctgct taaagcggtt gagtactgga taactccgcc gcttttcgaa aaagatttgg
    13621 gaaacagttg tcggcatccc gtgcaaatca ccatcggcaa accggaggag ctgcaacggg
    13681 tcagccaggt cagtagtggc atcagtttag gtttttgcta tttaacgttg cgcaaaagtc
    13741 cccgactctc cctctggcag gcgcgaaaag tgatctccat tattcatcaa tccggtttat
    13801 tacaaacgtt agaagtcgga gaaaacctga tcaccgccag tcatgcatta ctgccaggct
    13861 ggactattcc gtactggcaa gtaccggatg aagtcaaact accgaaaacc ttgacgctgg
    13921 tttatcacct accgatagaa cttcatacca tggcagaacg cctacaggcg acactggcag
    13981 cggaaggctg tgaactcaca attatttttc ataacgcaaa aaactgggac gacacgaccc
    14041 tactggcaca cgcagacctc atgatgggcg acagattaat tggcgaagca ccggaatata
    14101 ctctggagca atggctacgt tgcgatccac tatggccaca tgttttcgac gctccagcat
    14161 atgcacatct gcaatcgacg ctggacgcgg tgcaagtaat gcctgatgag gaaaaccgat
    14221 ttaatgccct gaaagcggtt tttagccagt taatggcaga cgcgacgctg acgccgctgt
    14281 tcaactatca ctatcgcatt agtgcccctc ccggcgtgaa cggtgtgcga ctgacaccgc
    14341 gcggctggtt tgaatttacc gaagcctggc ttcccgcgcc gtcgcaatga atagctggtc
    14401 cgggttaatc gcaggcgtta ccataacgct tttattgttc aagcaggatt atctatgaaa
    14461 cgtgctgtcg ttgtgttcag tggaggccag gattccacca cctgtctggt gcaggcatta
    14521 caacaatatg atgaagtcca ttgcgtgacg ttcgattacg gtcagcgaca tcgcgcagaa
    14581 atcgacgtgg cacgcgaact ggcgctgaaa ctgggagcac gcgcgcataa ggttcttgat
    14641 gtaacgctgc tcaacgagct ggcggtcagc agcctgacac gtgacagcat tccggtgcct
    14701 gattatgaac ctgaagccga tggcatcccg aatacgtttg tccctgggcg taatattttg
    14761 ttcctgacgc tggcggcaat atatgcgtat caggtaaaag cagaagccgt aattaccggc
    14821 gtctgtgaaa cggatttctc cggctacccg gattgccgcg atgagtttgt gaaagcacta
    14881 aaccatgccg tcagtttggg catggcgaaa gatattcgtt ttgaaacgcc gctgatgtgg
    14941 attgataaag cggaaacctg ggcgctggca gattattacg gcaaactgga tttagtccgt
    15001 aacgaaacgt tgacctgcta taacggcttt aaaggcgacg gttgcggtca ttgtgcggca
    15061 tgtaatttac gcgccaacgg tttgaatcat tatctggccg ataaaccgac ggtgatggca
    15121 gcgatgaagc agaaaaccgg gttgaggtaa ttattccggg tgtcgccgga tgcggcttga
    15181 gcatccggca ccacaaaacg tttacttaac catctgctcc agcttttcgc gcaattcccc
    15241 ttccagagct aatgctttct gcgttttaag atcaatacaa acaaacgtaa taagcgcatc
    15301 cgctaccacc tgcccttccg gctccagtgt aatgacctgg cttaagatgc cgcttttacc
    15361 gtttaattgc tgcaactgac tggtaatagt taacaggtcg cttaataccg ctgggcgacg
    15421 atagttaata ttgatattga ccacgacgaa ggcgatgtta tgggccgtca tccactgaaa
    15481 actgtcgcta ttttccaacc catcccagcg ggcttcttcg agaaattcaa ggtagcgggc
    15541 gttgttgacg tgctggtaaa cgtcgagatg atatccacga actttgattt gtgtttgcat
    15601 agcgcaataa ccttacgttg ttgttataag tacagaggtc ataactggta tgacctcttc
    15661 agtctggcaa aaaattgcca ctatgcaaat taattacagg gttaataccg ccagattacg
    15721 ttccaccagc gaattgccca tccccggcac ctgctttaga tcctccaccg ttttaaacgg
    15781 accgtactct tcgcgataac tgacaatcgc ctgcgctttc ttcaggccaa cgccattcat
    15841 cgcgcgggct agctcttccg cgctggcatt attaatgctg acccgggtgc cttcttcgtc
    15901 actggctttc gccggtactg ctgctttact ttgtgctgca ggagcttccg ctttggtttc
    15961 taccgccgtc ggtttcgcca cagaagctgc cgccagcgcg ctatgagaca ttccggcaca
    16021 ggccagggac agggtaatga gcagtgcttt aattccgtgt ttcatactgt tttctccttg
    16081 tttgttaaca gtggaatcac cttaacggga gtgaaaacag taaacaaatg gcaatgttca
    16141 gaaatggaaa aggccgcgaa agcgaccttt tcttgttaca gctgcaacgc atttttttgc
    16201 gaggcggctt caggattatt gctgttccag cgcatcgcca attttgattt tcgcctcttt
    16261 acgcaggtta ctcatcagag cttcaaagac gatttgtgcg ttgttctggg tgataccctg
    16321 caccatcgct tttttctgat cttccggcat tgaaccttgt ttcacttcat ccagcgccag
    16381 cagaaccaca ttaccttgca tatcggtcgc cataccgtag ctcggtttgt ctttcgctgg
    16441 cagtggcagt gcaaacgccg cctggctaat cgggtcacga ccggaacggc ttaagatttt
    16501 cggctcgcca aatttcagac cggcagcctg catagcttcc gcacctttgc cggctttcaa
    16561 atcaaccagc agtttctcag catccacttt cgcctgttgt tcagctttgt tgtgctgaac
    16621 cagtgcctta acttgttcct gaacatctgc caacggtttc accgcttccg gtttgtgctc
    16681 gctgatgcgc agcacgaatg cgcggtcgcc gtctacggtg atgatgtcag agttgatgcc
    16741 cggcgcgccg ttttcaccta ccagaccgcc gttaaagata gcgtcggcaa ccggcttgaa
    16801 gttcaactct tccggcaggt tatctttgct aaaccaaccc gtctgagtgg ctttaacgcc
    16861 ggcagcttgc tctgcaccgg ccagagactc cgtgtcgttg cttgccgcat cgctcacttt
    16921 ctgctgcagc gcatagtacg catcgagggc tttttcgtgt ttcactttcg ccgcaatgtc
    16981 gtcacgtact tcgtctaacg atttcacttt cgctggctga atgtcgtcca gacgtacaat
    17041 caggaaaccg accgaagatt tgatgacacc agacagttgc cctttttctt tcagaccagc
    17101 atttttcagt tcgtcgggga tagtggcatc ttctaaccaa cccatatcgc cgccgttacg
    17161 ggcagagata atatcggcag atttttcttt ggctaatgca gcaaaatcac cgcctttatt
    17221 cagctcatca agtaccactt tcgcttcatc ttcagttttg gtctggatga tgctgtagcg
    17281 ggtacgctgc ggctgggtga attgatcctg atgctgatcg tagtagctct ggatatccgc
    17341 atcgctaacc ggttgctgca tcgttgcggc atccagcttg atgtaactca cgcggaattg
    17401 ttccggcgtc atgaaattgt ttttgttttg ttcgtagtag ctggcaatct cctgttcggt
    17461 cacaggctgc ttcgccgcca gcgcgttaac atcgatagtc gcctcacgca ccacgcgttg
    17521 ttgcgcgacc agtgccgcca gctcgtcggt ttcacctttc agcataaaat cggtaccggc
    17581 aacaccgtta atcagctgtt gggtagtgag ctggttacgc agcgcctggg cgtactgatc
    17641 ggcggtcatc cccatctggt tgaggatacc gttatagcgg ctgttatcaa atttgccgtc
    17701 aacctggaag gctggggtcg cgaaaatcgc ctgtttcacc tgctcatcac tgatacccag
    17761 tttcagctca cgagcgtact gatccaacag cgcctcgtcg atcagacgat tcagcacctg
    17821 ttgacgcagg gttttcatat agccttcgtt cgctgccagc tcggagtatt gatcgcccag
    17881 ctgttgctgc atgcgattac gctcgctgtt aaaggcgttc tcgaactgcc cacggctgat
    17941 ttcctggtca ttcacttttg cggcgtagtt attgcctccg ccaatcaggt aaccactcac
    18001 gccggtcaat atgaacgaca cgataatgat accgaaaata atcttgagca cgagactgtt
    18061 tgcagccgtg cgtaagctgt ccatcatggt gtaacaacac tccgctgtag atgactgtgt
    18121 acctcacgca acgtatcgat gcgcgcaaag gcctattgtg acaagaaacg ggggcaattg
    18181 tcagcccaca agacacagaa agtcaccgaa tacaaataaa aaaggcacat cagtagatgc
    18241 gcccttgaac ttcgtcacat ccccactggg gacaacgctt agtttaccgc gtctttcagt
    18301 gctttacctg cacggaagct cggtacttta gcagcagcga tggtgatctc tttaccggtc
    18361 tgcgggttgc ggccagtacg ggcagcacgc tctttaacgg caaaagtacc aaaacctacc
    18421 agtgctacat catccccttc tttcagagat tcagttacgg aagcaataat agcatctaac
    18481 gcacggccag ccgcagcttt agagatatca gcccctgcag caatcttgtc gatcaattga
    18541 gatttattca ctcttctctt cctctttata atttatatcg cacttgaatc cttcaaggta
    18601 cgaacgcgca gcagttatat caggccagcc atccccttac aacacccctt aataagggca
    18661 agcccgatcc gccatctaac ttagcgagac aaaaaaaggc tggcaagccc gaattagcct
    18721 gccagccctg tttttattag tgcattttgc gcgaggtcac tattttgcag tcacaacctg
    18781 catgccagac ggttcatttt gcagcgccag agtcagaact tcctcaatgc gcttcacagg
    18841 atgaatgtcc agatcggcaa ttacgttgtc aggaatctct tccagatcgc gtttattttc
    18901 gaacggaatt agcactgttt taatcccgcc gcgatgcgct gccaggagtt tttctttcaa
    18961 accaccgatc ggcagtacct gaccacgcag agtgatctca ccggtcattg ccacatcggc
    19021 acgaaccggg ttaccggtca ggcaagaaac cagcgcggtg cacatagcaa taccggcact
    19081 cggaccatct ttcggcgtcg caccttccgg tacgtggacg tggatgtcac gtttttcgta
    19141 aaaatcaggg ttgatcccca gtttttccgc acgcgcacga accaccgtta acgccgcctg
    19201 aatagactcc tgcatcactt cgccgagcga accggtatag gtcagtttgc ctttacccgg
    19261 aacacatgcg gtttcaatgg tcagcaagtc accgcccact tccgtccacg ccagaccggt
    19321 tacctgaccg acacggtttt cgttatcagc gcgaccatag tcgaaacgct gaacaccgag
    19381 gtagtcatgc aggttatcgc cgttaatttc gatatgtttt aatgacttat cgagcagtaa
    19441 ctgcttaacc gctttgcggc acagtttgga gatttcacgc tccagaccac gcacgcccgc
    19501 ctcacgggtg tagtaacgaa taatgccgat aatggcgcta tcgtcgacgg tcagctcacc
    19561 ttttttcagt gcattacgtt caatctgctt cggcagcagg tgacgtttgg cgatgttcag
    19621 tttttcatct tcggtataac cggagaggcg aatcacttcc atacgatcca gcagcggtgc
    19681 cggaatgttc atggagttcg acgtcgcgac aaacatcacg tcgctgagat cgtaatccac
    19741 ttccaggtag tggtcgctga acgctacgtt ctgctctgga tccagcactt caagcagtgc
    19801 agaggccgga tcgcctcgca tgtcagaaga cattttgtcg atctcatcga gcaggaacag
    19861 cgggtttttc acgcccactt tcgccatttt ctggatcagt ttacccggca tagaaccgat
    19921 gtaagtacgg cggtgaccac ggatttccgc ttcatcacgc acgccgccca gcgccatacg
    19981 gacatattta cgcccggtgg ctttggcaat ggactgacca agagaggttt tacctacccc
    20041 cggcggccct accaggcaga ggatcggtcc cttgattttg ttgacacggc tttgaaccgc
    20101 aaggtactca aggatgcgat ctttcacgcg ctccagacca taatggtcgg tatcaaggat
    20161 ttcctgcgcc tgacgcaggt cttttttgac cttgctgcgc gcattccacg gtacctgtac
    20221 catccagtcg atataaccac gcactacggt cgcttctgcc gacatcggag acatcatttt
    20281 cagcttctgc aactctgctt ccgctttctc ttttgcctct ttcggcattt tcgccgcgtc
    20341 gattttgcgc ttcagggctt cgttttcgtc cggcgcgtcg tccatttcac cgagttcttt
    20401 ctgaatagct ttcatttgct cgttcagata gtactcacgc tgggatttct ccatctgctt
    20461 tttaacgcgg ttgcgaatgc gtttctcaac ctgcagcaga tcgatttccg attccatcat
    20521 tgccatcaga tattccagac gttcgttaac gtcggacatc tccagaacgg actgtttgtc
    20581 agccagtttc agcggcatat gtgcagcaat ggtatccgcc agacgcgccg gatcgtcgat
    20641 gctattcagc gacgtcagca cttctggtgg gatttttttg ttcagcttga tgtagccttc
    20701 gaactggctg attgcagtac gcaccagcac ttcctgttcc cgctcatcaa tggtcggcga
    20761 ctccagatac tccgccttcg cagaaaagtg ttcgccattg tcagagagcg cagaaatacg
    20821 cgcgcgctgt aacccctcga ccagcacttt gacggtgccg tcaggcagtt tcagcatctg
    20881 caatatagag gccacggtcc cgacggtgaa aagatcgttt acacccggct catccgttga
    20941 agcttctttc tgcgcgacca gcataatttt tttatcatgg tccatcgccg cttccagaca
    21001 acggatagat ttttcccgcc cgacaaataa ggggatgacc atgtgcggat aaaccaccac
    21061 atcgcgcagc ggcaatacgg ggatttcaat gcgttcagaa cgctcaggat tcatagagct
    21121 ctctcttagt ttaatttccg ccaggtaatc agatgacacg actgtgcttc acgccatcta
    21181 ttaacatgta cgtcagtata tggggatgtt tcccccacat tcaacgccga gaatagagga
    21241 aaaattaaag gggagataaa atcccccctt tttggttaac taattgtatg ggaatggtta
    21301 attattcacc agatgcctgt tgcgcttccg gcttaccata aatcagcaac ggtttgcttt
    21361 gaccatcaat taccgactcg tcgataacca ctttttcgac atcttccatg gacggcagat
    21421 cgtacatggt atcgagcagt gcggcttcta cgatggaacg caggccacgg gcaccggttt
    21481 tacgcgccat cgctttctta gcgatagcat ccagcgcctc gtcacggaat tccagatcca
    21541 cgccttccag attaaacagc gcctgatact gcttggtcag ggcgtttttc ggctctttga
    21601 ggatctgaat cagagcttct tcgctcagtt cattcaacgt tgcgacaacc ggcagacgac
    21661 caataaactc agggataaga ccaaacttga tcagatcttc cggttcaacc tgcgccagca
    21721 gctcgccttc gcttgctttg tcggacttcg cttttaccgt cgcgccaaaa ccaatgccgg
    21781 agccggtttc tacacggtgg gaaatcactt tatccagacc ggcaaacgca ccgccacaaa
    21841 taaacaggat cttagaggta tcaacctgca agaattcctg ctgcggatgt ttacgcccac
    21901 cttgcggtgg aacagcagct accgtacctt cgatcagttt caacagtgcc tgctgtacgc
    21961 cttcaccgga aacgtctcgg gtaatggacg ggttgtctga cttacgagaa atcttgtcga
    22021 tctcatcgat gtagacaatc ccgcgctgcg ctttctgtac gtcgtaatcg cacttctgca
    22081 acagcttctg aatgatgttt tcaacgtctt cgcccacata accggcttcg gtcagcgtgg
    22141 ttgcgtcggc catggtgaac gggacgtcca gcaggcgcgc cagcgtttca gccagcagcg
    22201 ttttaccgga accggtcgga ccgatcagca gaatgttact tttgcccaac tcgacaccat
    22261 tgctggtatc gccgttgcgc agacgtttgt aatggttgta taccgcgacc gccagcactt
    22321 ttttcgcctg ttcctggccg ataacgtaat cgtccaggtg gttgcgaatt tcatgcggcg
    22381 tcggtagcgc actgcgttca cgatgcggtg caacttcttt aatctcttcg cgaatgatgt
    22441 cgttacataa atcaacacat tcgtcgcaga tatacacgga tggaccggca atcagcttgc
    22501 gcacttcatg ctggcttttg ccgcaaaaag agcaatacag caatttgcct gagccatctt
    22561 tgcgtttatc tgtcatgggt caaaacctct tctttgttct ttgtgccgca cacgacgacg
    22621 caaatgccat tctcaggcgc aagccgctta cagcgttgtg ccgccctgga taagtatagc
    22681 ggcacagttg cgcctctggc atcaattacg atgggtcaga atcgaatcga ccagaccgta
    22741 ttccaccgct tcaggggcgg aaaggaagcg atcgcgctcg gtatcacgtt caatctgttc
    22801 taatgattga cccgtatgaa gcgccataag ttcattcatg cgccctttaa ctttcagaat
    22861 ttcacgggca tgaatttcga tatcggtcgc ctggccctgg tagccgccca acggttggtg
    22921 aatcatcacg cgcgaattcg gcaggcaaaa acgtttacct tttgcccctg cggtcaacaa
    22981 gaaagcgccc atcgaggccg cctggcccat acagatggtg ctgacatcag gcttgataaa
    23041 ctgcatggtg tcatagatag acatcccggc agtgatcacc ccgcctgggg agttaatgta
    23101 cagatagata tctttttctg ggttttccgc ttccaggaac agcatctgcg ccacaatcag
    23161 gttagccatg tggtcttcaa cctggccagt cagaaaaatg acgcgttcct taagtagacg
    23221 agaatagata tcaaaagagc gctcaccgcg tgaggtctgt tcaatgacca tcggcaccag
    23281 cgccatatgg ggtgcaaagt tatctcgttc gccgctgtat gacatttccg tctcctggat
    23341 aaaattgaaa aaacctgctg taccgattgt aaccttatgg acggattatc agctagtccc
    23401 tgtgttatgg gtacggcatc accatatttc aagcataaca atcttttgtt gttacgctaa
    23461 caccgaaaga ggcttttcgc acggttccat gcaaaattca tgacaaaaaa aaagcccacc
    23521 acctggcggt gacgggcctt tgtgcgaatt tagcgcgtta tgctgcgtaa attacgcctg
    23581 ctggttcatc agctcgttga aagtggtttc tttttcagtc actttcgctt tcgccagtac
    23641 agcttcaaca gcctgttctt ccagagcaac attgcgcatg ttgtccatca gttctttgtt
    23701 tttgctgtag aactcgataa cttctttcgg atcttcgtac gcagaagcca tctcttcgat
    23761 caggcctttc acgcgctctt cgtcagcttt cagctcgttg gtgcggataa cttcgcccag
    23821 cagcaggcca acaactacgc ggcgtttagc ctgttcttcg aacagttcgc gcggcagttc
    23881 cagagcttgt ttttcgttgc caccgaaacg ctgtgcagcc tggcgacgca gaacgtcgat
    23941 ttcgctgtcg atcagcgcag ccggtacgtc gatgtcgtta gcttttacca gaccttcgat
    24001 cgcctgagac ttaacgcggt tacggatggc gctcttcagc tcgcgctcca tgtttttacg
    24061 cacttcagcg cgcagacctt ctacggaacc atcttcaacg ccgaaacgtt tgatgaattc
    24121 tgcagtcagt tccggcagtt cacgctcttc aactttcttc aggttgatag cgaatttcgc
    24181 tgctttacct ttcaggtttt ctgcgtggta ttcttccggg aaggtcacgt cgatggtgaa
    24241 ctcttcgcca gctttgtggc ctttgatacc gtcttcaaag cccgggatca tacgaccctg
    24301 gcccatcgcc agtacgaaat cagacgcttt accgccttcg aactcttcgc cgtctacaga
    24361 accggtgaag tcgatggtta cgcggtcttc tgcttcaaca gcgccgtctt tttctttcca
    24421 ggtcgcctgc tgtttacgca gagtatccag catgccgtca acgtcagcgt cggtcacttc
    24481 aacgatcggt ttttcaactt cgatcgcttc cagaccctgc agttcaactt ccggataaac
    24541 ttcaaactct acagagtaag tgaagtcttc acccagcttg tattcgcccg gaacataagt
    24601 cggtgcgcca gccggattga ttttttcttt aatgatggcg tcaatgaagt tacggctcat
    24661 caggtcaccc agaacgtcct ggcgtacaga cgcgccataa cgctgagcaa cgatattcat
    24721 tggcactttg cctttgcgga agccgtcaat acgtactttt ttcgcaacgt tgaccagctc
    24781 gcttttaaca gcggtctcga tgctgtcagc agcgatagta atcgttacac ggcggccaag
    24841 gccttgagtg gtttcaactg aaacttgcat cttgttacct caaaaaatca cagtgctcgg
    24901 tcaactctta ccccgcaagc acaggttgtt ggcttcgcgg aaccgggatg ttcttataat
    24961 caatcacatt ccctgttacc agaatcatcc cgaaagcatt caaataggac gcggcattat
    25021 agcggcatca cttttatgag tcgagaacgg ttattgcgcg ctgtcgcgta ttttttcaca
    25081 attcccgggc aattttcgtc tgaatgctgg gcaaaaagcg caaaatccag acaaaacaaa
    25141 acggcccgca ggccgttttt cataaaatct ttagcaacat actggaaaag cgccgacagt
    25201 tgcaaatgcg tttttacgcg atgcttcctg ctccacgaca gggaggagag gcaaagacgg
    25261 tgtcctgcaa cccttcccac tccttaatag tgtaagtatg cagagccagc gcatgaacgg
    25321 tagtagagag ttcctccgct aaagtactgt aaatcattcg atgacgattc agaaaacgtt
    25381 cacccgtaaa acgatcgctg accagcacaa ctttaaaatg gctttcagag ccggctggga
    25441 cattgtgacg atagctttca tccactactt cgaggaatac gggttggaac gccgccctta
    25501 atttttcttc tatccgctca cgtatcatca tgaaatatct cctccgacaa cgctgagatg
    25561 tcatccatcc ctttaaatac tagccgcttt taccgtttcc attgcagctt aacaacaaat
    25621 atttaggttt cgtcctggtt tttccgtcaa gcgtatgaag tttactgatt taaagattga
    25681 aacacaggcc tcgcatcagc gtttctttta ccaggggtga ttatttcgtc aacatgatga
    25741 atttacatgc cttacccact tcccctcgcc gttggcaatg ttatgatggc gggaattttt
    25801 accaaacacg cttaagagtc tctgagagcc cgaacatgtt caaaaaaatc ctcttcccgt
    25861 tagttgctct gtttatgctt gcaggatgcg caaaaccgcc aacaactatt gaagtttccc
    25921 cgacgattac actgccacag caggatccaa gcctgatggg cgtcaccgta agcattaatg
    25981 gtgccgatca gcgtaccgat caggcgctgg caaaagtcac ccgcgataat caaatcgtta
    26041 ccctgaccgc ctcccgcgat ctgcgtttcc tgctgcaaga agtgctggaa aaacagatga
    26101 ccgcgcgtgg ttacatggtt ggtccgaatg gcccggttaa tctgcaaatc atcgttagcc
    26161 aactgtatgc tgacgtgtcc cagggcaacg tgcgctacaa catcgctacc aaagcggata
    26221 tcgccatcat cgctaccgcg cagaatggca acaaaatgac caaaaactat cgtgccagct
    26281 acaatgttga aggtgcgttc caggcctcca acaaaaatat cgccgatgcg gttaacagtg
    26341 tgctgaccga taccatcgct gatatgtctc aggacaccag cattcacgaa ttcatcaagc
    26401 agaacgcgcg ttaatttctg ccctctggcc cggtgcaagc cgggcctgta gacgcccatg
    26461 tccagtcaat atttacgtat ttttcaacag ccgcgttcag ccatattgct gatcctgggt
    26521 tttgcttccg ggctaccgct cgccctgaca tccggcacct tacaggcctg gatgacggtc
    26581 gagaatattg atctcaaaac cattggtttc ttctctctgg taggccaggc ttacgttttt
    26641 aaattcctct ggtcaccgct gatggaccgc tacacgcccc cattttttgg gcggcggcgc
    26701 ggttggctgc tcgccacgca aatcctgtta ttagtcgcca ttgcggcgat gggttttctc
    26761 gaacctggca cccaactccg ctggatggcg gcactggcgg tggtgatcgc tttttgctcg
    26821 gcctcccagg atattgtctt cgatgcgtgg aaaaccgatg tgctcccggc agaagaacgt
    26881 ggtgcgggcg cggcaatcag cgtgctgggt taccgtttag ggatgctggt ttccggcggc
    26941 ttagccctgt ggctggcaga taaatggctg ggctggcagg gcatgtactg gttgatggcg
    27001 gcgctgttga tcccttgtat tatcgcgacg ttgcttgcac cagaaccgac cgacaccatt
    27061 cctgtgccaa aaacgctgga acaagcggtt gttgcacctc tgcgagattt ctttggtcgc
    27121 aataatgcct ggcttatttt gcttcttatc gtgctgtata agctgggcga cgcattcgcc
    27181 atgagcctga caaccacgtt tttgattcgc ggcgtcgggt ttgatgcggg tgaagtaggc
    27241 gtggttaaca aaacgcttgg cttactggcg accattgtgg gcgcattgta cggtgggatt
    27301 ttgatgcagc gcctgtcgct gttccgggcg ctgctgattt tcggcatttt acaaggtgcg
    27361 tctaacgctg gttactggct gctgtcgatt actgataagc atctctacag catgggcgca
    27421 gccgtctttt tcgaaaacct ctgtggcggg atgggcacat cagcctttgt cgcgctgtta
    27481 atgacgctgt gtaataagtc attttccgca actcaatttg ccctgctctc agcgctttcc
    27541 gctgtaggac gagtttatgt cggccccgtg gcgggttggt ttgttgaagc acacggctgg
    27601 tcgacattct atctattctc cgtcgccgct gccgtaccgg ggcttatttt gctgctggtt
    27661 tgccgccaga cgcttgaata tacaggagta aatgacaact ttatctcccg taccgaatat
    27721 ccggcaggtt atgcctttgc catgtggaca ctggcggcgg gcgtcagcct gttggccgtg
    27781 tggttactgc tgttgacgat ggacgcgctg gatttgacgc acttctcttt cctgcctgct
    27841 ctgctggaag tcggggtttt agtcgccctt tctggcgtcg tgcttggtgg tttgctggat
    27901 tatctggcgc tacgaaaaac gcatctgacg taatctgtaa atattattta gctgtagtaa
    27961 tcactcgccg taattattac ggctaaataa acatcagtat cgcttaataa t
//
LOCUS       DAZKHF020000034        27166 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2201S31241
            SAMN43267800-rid25106413.denovo.034, whole genome shotgun sequence.
ACCESSION   DAZKHF020000034 DAZKHF020000000
VERSION     DAZKHF020000034.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267800
            Sequence Read Archive: SRR30307263
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 27166)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 27166)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 27166)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350581.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350581.2
            Genome Coverage       :: 151x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:21:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,652
            CDSs (total)                      :: 5,557
            Genes (coding)                    :: 5,320
            CDSs (with protein)               :: 5,320
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 237
            CDSs (without protein)            :: 237
            Pseudo Genes (ambiguous residues) :: 0 of 237
            Pseudo Genes (frameshifted)       :: 102 of 237
            Pseudo Genes (incomplete)         :: 130 of 237
            Pseudo Genes (internal stop)      :: 52 of 237
            Pseudo Genes (multiple problems)  :: 41 of 237
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27166
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267800-rid25106413.denovo.034"
                     /serotype="O 26:H11"
                     /isolate="2201S31241"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-01"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            complement(151..813)
                     /gene="ytfE"
                     /locus_tag="ACHOSC_RS05970"
                     /old_locus_tag="pgaptmp_001194"
     CDS             complement(151..813)
                     /gene="ytfE"
                     /locus_tag="ACHOSC_RS05970"
                     /old_locus_tag="pgaptmp_001194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster repair protein YtfE"
                     /protein_id="HGU4547170.1"
                     /translation="MAYRDQPLGELALSIPRASALFRKYDMDYCCGGKQTLARAAARK
                     ELDVEVIEAELAKLAEQPIEKDWRSAPLAEIIDHIIVRYHDRHREQLPELILQATKVE
                     RVHADKPSVPKGLTKYLTMLHEELSSHMMKEEQILFPMIKQGMGSQAMGPISVMESEH
                     DEAGELLEVIKHTTNNVTPPPEACTTWKAMYNGINELIDDLMDHISLENNVLFPRALA
                     GE"
     gene            complement(921..1886)
                     /gene="ytfF"
                     /locus_tag="ACHOSC_RS05975"
                     /old_locus_tag="pgaptmp_001195"
     CDS             complement(921..1886)
                     /gene="ytfF"
                     /locus_tag="ACHOSC_RS05975"
                     /old_locus_tag="pgaptmp_001195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709987.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HGU4547171.1"
                     /translation="MISGVLYALLAGLMWGLIFVGPLIVPEYPAMLQSMGRYLALGLI
                     ALPIAWLGRVRLRQLARRDWLTALMLTMMGNLIYYFCLASAIQRTGAPVSTMIIGTLP
                     VVIPVFANLLYSQRDGKLAWGKLAPALICIGIGLASVNIAELNHGLPDFDWARYTSGI
                     VLALVSVVCWAWYALRNARWLRENPDKHPMMWATAQALVTLPVSLIGYLVACYWLNIQ
                     TPDFSLPFGPRPLVFISLMVAIAVLCSWVGALCWNVASQRLPTVILGPLIVFETLAGL
                     LYTFLLRQQMPPLMTLSGIALLVIGVVIAVRAKPEKPLTESVSES"
     gene            complement(1994..2854)
                     /gene="qorB"
                     /locus_tag="ACHOSC_RS05980"
                     /old_locus_tag="pgaptmp_001196"
     CDS             complement(1994..2854)
                     /gene="qorB"
                     /locus_tag="ACHOSC_RS05980"
                     /old_locus_tag="pgaptmp_001196"
                     /EC_number="1.6.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H:quinone oxidoreductase"
                     /protein_id="HGU4547172.1"
                     /translation="MIAITGATGQLGHYVIESLKKTVPVSQIVAIVRNPAKAQALAAQ
                     GITVRQADYGDEAALTSALQGVEKLLLISSSEVGQRAPQHRNVINAAKAAGVKFIAYT
                     SLLHADTSPLGLADEHIETEKMLADSGIVYTLLRNGWYSENYLASAPAALEHGVFIGA
                     AGDGKIASATRADYAAAAARVISEAGHEGKVYELAGDSAWTLTQLAAELTKQSGKQVT
                     YQNLSEADFAAALKSVGLPDGLADMLADSDVGASKGGLFDNSKTLSKLIGRPTTTLAE
                     SVSHLFNVNN"
     gene            2943..3323
                     /gene="ytfH"
                     /locus_tag="ACHOSC_RS05985"
                     /old_locus_tag="pgaptmp_001197"
     CDS             2943..3323
                     /gene="ytfH"
                     /locus_tag="ACHOSC_RS05985"
                     /old_locus_tag="pgaptmp_001197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418633.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix transcriptional
                     regulator"
                     /protein_id="HGU4547173.1"
                     /translation="MSQVSLSQQLKEGNLFAEQCPSREVLKHVTSRWGVLILVALREG
                     THRFSDLRRKIGGVSEKMLAQSLQALEQDGFLNRIAYPVVPPHVEYSLTPLGEQVSEK
                     VAALADWIELNLPEVLAVRDERAA"
     gene            complement(3452..5395)
                     /gene="cpdB"
                     /locus_tag="ACHOSC_RS05990"
                     /old_locus_tag="pgaptmp_001198"
     CDS             complement(3452..5395)
                     /gene="cpdB"
                     /locus_tag="ACHOSC_RS05990"
                     /old_locus_tag="pgaptmp_001198"
                     /EC_number="3.1.4.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008663 - 2',3'-cyclic-nucleotide
                     2'-phosphodiesterase activity [Evidence IEA];
                     GO_process: GO:0009117 - nucleotide metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2',3'-cyclic-nucleotide 2'-phosphodiesterase"
                     /protein_id="HGU4547174.1"
                     /translation="MIKFSATLLATLIAASVNAATVDLRIMETTDLHSNMMDFDYYKD
                     TATEKFGLVRTASLINDARNEVKNSVLVDNGDLIQGSPLADYMSAKGLKAGDVHPVYK
                     ALNTLDYTVGTLGNHEFNYGLDYLKNALAGAKFPYVNANVIDARTKQPMFTPYLIKDT
                     EVVDKDGKKQTLKIGYIGVVPPQIMGWDKANLSGKVTVNDITETVRKYVPEMREKGAD
                     VVVVLAHSGLSADPYKVMAENSVYYLSEIPGVNAIMFGHAHAVFPGKDFADIEGADIT
                     KGTLNGVPAVMPGMWGDHLGVVDLQLSNDSGKWQVTQAKAEARPIYDIANKKSLAAED
                     SKLVETLKADHDATRQFVSKPIGKSADNMYSYLALVQDDPTVQVVNNAQKAYVEHYIQ
                     GDPDLAKLPVLSAAAPFKVGGRKNDPASYVEVEKGQLTFRNAADLYLYPNTLIVVKAS
                     GKEVKEWLECSAGQFNQIDPNSTKPQSLINWDGFRTYNFDVIDGVNYQIDVTQPARYD
                     GECQMINANAERIKNLTFNGKPIDPNAMFLVATNNYRAYGGKFAGTGDSHIAFASPDE
                     NRSVLAAWIADESKRAGEIHPAADNNWRLAPIAGDKKLDIRFETSPSDKAAAFIKEKG
                     QYPMNKVATDDIGFAIYQVDLSK"
     gene            5585..6325
                     /gene="cysQ"
                     /locus_tag="ACHOSC_RS05995"
                     /old_locus_tag="pgaptmp_001199"
     CDS             5585..6325
                     /gene="cysQ"
                     /locus_tag="ACHOSC_RS05995"
                     /old_locus_tag="pgaptmp_001199"
                     /EC_number="3.1.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008441 - 3'(2'),5'-bisphosphate
                     nucleotidase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3'(2'),5'-bisphosphate nucleotidase CysQ"
                     /protein_id="HGU4547175.1"
                     /translation="MLDQVCQLARNAGDAIMQVYDGTKPMDVVSKADNSPVTAADIAA
                     HTVIMDGLRTLTPDIPVLSEEDPPGWEVRQHWQRYWLVDPLDGTKEFIKRNGEFTVNI
                     ALIDHGKPILGVVYAPVMNVMYSAAEGKAWKEECGVRKQIQVRDARPPLVVISRSHAD
                     AELKEYLQQLGEHQTTSIGSSLKFCLVAEGQAQLYPRFGPTNIWDTAAGHAVAAAAGA
                     HVHDWQGKPLDYTPRESFLNPGFRVSIY"
     gene            complement(6315..6872)
                     /gene="ytfJ"
                     /locus_tag="ACHOSC_RS06000"
                     /old_locus_tag="pgaptmp_001200"
     CDS             complement(6315..6872)
                     /gene="ytfJ"
                     /locus_tag="ACHOSC_RS06000"
                     /old_locus_tag="pgaptmp_001200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001498728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtfJ family protein"
                     /protein_id="HGU4547176.1"
                     /translation="MTLRKILALTCLLLPMMASAHQFETGQRVPPIGITDRGELVLDK
                     DQFSYKTWNSAQLVGKVRVLQHIAGRTSAKEKNATLIEAIKSAKLPHDRYQTTTIVNT
                     DDAIPGSGMFVRSSLESNKKLYPWSQFIVDSNGVARGAWQLDEKSSAVVVLDKDGRVQ
                     WAKDGALTQEEVQQVMDLLHKLINK"
     gene            7197..7403
                     /gene="ytfK"
                     /locus_tag="ACHOSC_RS06005"
                     /old_locus_tag="pgaptmp_001201"
     CDS             7197..7403
                     /gene="ytfK"
                     /locus_tag="ACHOSC_RS06005"
                     /old_locus_tag="pgaptmp_001201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015966176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1107 domain-containing protein"
                     /protein_id="HGU4547177.1"
                     /translation="MKIFQRYNPLQVAKYVKILFRGRLYIKDVGAFEFDKGKILIPKV
                     KDKLHLSVMSEVNRQVMRLQTEMA"
     gene            complement(7465..8808)
                     /gene="paeA"
                     /locus_tag="ACHOSC_RS06010"
                     /old_locus_tag="pgaptmp_001202"
     CDS             complement(7465..8808)
                     /gene="paeA"
                     /locus_tag="ACHOSC_RS06010"
                     /old_locus_tag="pgaptmp_001202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin family protein"
                     /protein_id="HGU4547178.1"
                     /translation="MLNSILVILCLIAVSAFFSMSEISLAASRKIKLKLLADEGNINA
                     QRVLNMQENPGMFFTVVQIGLNAVAILGGIVGDAAFSPAFHSLFSRYMSAELSEQLSF
                     ILSFSLVTGMFILFADLTPKRIGMIAPEAVALRIINPMRFCLYVCTPLVWFFNGLANM
                     IFRIFKLPMVRKDDITSDDIYAVVEAGALAGVLRKQEHELIENVFELESRTVPSSMTP
                     RENVIWFDLHEDEQSLKNKVAEHPHSKFLVCNEDIDHIIGYVDSKDLLNRVLANQSLA
                     LNSGVQIRNTLIVPDTLTLSEALESFKTAGEDFAVIMNEYALVVGIITLNDVMTTLMG
                     DLVGQGLEEQIVARDENSWLIDGGTPIDDVMRVLDIDEFPQSGNYETIGGFMMFMLRK
                     IPKRTDSVKFAGYKFEVVDIDNYRIDQLLVTRIDSKATALSPKLPDAKDKEESVA"
     gene            complement(9131..9769)
                     /gene="msrA"
                     /locus_tag="ACHOSC_RS06015"
                     /old_locus_tag="pgaptmp_001203"
     CDS             complement(9131..9769)
                     /gene="msrA"
                     /locus_tag="ACHOSC_RS06015"
                     /old_locus_tag="pgaptmp_001203"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008113 - peptide-methionine (S)-S-oxide
                     reductase activity [Evidence IEA];
                     GO_function: GO:0033744 -
                     L-methionine:thioredoxin-disulfide S-oxidoreductase
                     activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HGU4547179.1"
                     /translation="MSLFDKKHLVSPADALPGRNTPMPVATLHAVNGHSMTNVPDGME
                     IAIFAMGCFWGVERLFWQLPGVYSTAAGYTGGYTPNPTYREVCSGDTGHAEAVRIVYD
                     PSVISYEQLLQVFWENHDPAQGMRQGNDHGTQYRSAIYPLTPEQDAAARASLERFQAA
                     MLAADDDRHITTEIANATPFYYAEDDHQQYLHKNPYGYCGIGGIGVCLPPEA"
     gene            9975..11708
                     /gene="tamA"
                     /locus_tag="ACHOSC_RS06020"
                     /old_locus_tag="pgaptmp_001204"
     CDS             9975..11708
                     /gene="tamA"
                     /locus_tag="ACHOSC_RS06020"
                     /old_locus_tag="pgaptmp_001204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter assembly complex protein TamA"
                     /protein_id="HGU4547180.1"
                     /translation="MRYIRQLCCVSLLCLSGSAVAANVRLQVEGLSGQLEKNVRAQLS
                     TIESDEVTPDRRFRARVDDAIREGLKALGYYQPTIEFDLRPPPKKGRQVLIAKVTPGV
                     PVLIGGTDVVLRGGARTDKDYLKLLDTRPAIGTVLNQGDYENFKKSLTSIALRKGYFD
                     SEFTKAQLGIALGLHKAFWDIDYNSGERYRFGHVTFEGSQIRDEYLQNLVPFKEGDEY
                     ESKDLAELNRRLSATGWFNSVVVAPQFDKARETKVLPLTGVVSPRTENTIETGVGYST
                     DVGPRVKATWKKPWMNSYGHSLTTSTSISAPEQTLDFSYKMPLLKNPLEQYYLVQGGF
                     KRTDLNDTESDSTTLVASRYWDLSSGWQRAINLRWSLDHFTQGEITNTTMLFYPGVMI
                     SRTRSRGGLMPTWGDSQRYSIDYSNTAWGSDVDFSVFQAQNVWIRTLYDRHRFVTRGT
                     LGWIETGDFDKVPPDLRFFAGGDRSIRGYKYKSIAPKYANGDLKGASKLITGSLEYQY
                     NVTGKWWGAVFVDSGEAVSDIRRSDFKTGTGVGVRWESPVGPIKLDFAVPVADKDEHG
                     LQFYIGLGPEL"
     gene            11705..15484
                     /gene="tamB"
                     /locus_tag="ACHOSC_RS06025"
                     /old_locus_tag="pgaptmp_001205"
     CDS             11705..15484
                     /gene="tamB"
                     /locus_tag="ACHOSC_RS06025"
                     /old_locus_tag="pgaptmp_001205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter assembly complex protein TamB"
                     /protein_id="HGU4547181.1"
                     /translation="MSLWKKISLGVVIVILLLLGSVAFLVGTTSGLHLVFKAADRWVP
                     GLDIGKVTGGWRDLTLSDVRYEQPGVAVKAGNLHLAVGLECLWNSSVCINDLALKDIQ
                     VNIDSKKMPPSEQVEEEEDSGPLDLSTPYPITLTRVALDNVNIKIDDTTVSVMDFTSG
                     LNWQEKTLTLKPTSLKGLLIALPKVAEVAQEEVVEPKIENPQPEEKPLGETLKDLFSR
                     PVLPEMTDLHLPLNLNIEEFKGEQLRVTGDTDITVRTMLLKVSSIDGNTKLDALDIDS
                     SQGSVNASGTAQLSDNWPVDITLNSTLNVEPLKGEKVKLKVGGALREQLEIGVNLSGP
                     VDMDLRAQTRLAEAGLPLNVEVNSKQLYWPFTGEKQYQADDLKLKLTGKMTDYTLSMR
                     TAVKGLEIPPATITLDAKGNEQQVNLDKLTVAALEGKTELKALLDWQQAISWRGELTL
                     NGINTAKEIPEWPSKLNGLIKTRGSLYGGTWQMEVPELKLTGNVKQNKVNVDGTLKGN
                     SYMQWMIPGLHLELGPNSAEVKGELGVKDLNLDATINAPGLDNALPGLGGTAKGLVKV
                     RGTVEAPQLLADITARGLRWQELTVAQVRVEGDIKSTVQIAGKLDVRVEQISQPDVNI
                     NLVTLNAKGSEKQHELQLRIQGEPVSGQLNLAGSFDRKEERWKGTLSNTRFQTPVGPW
                     SLTRDIALDYRNKEQKISIGPHCWLNPNAELCVPQTIDAGAEGRAVVNLNRFDLAMLK
                     PFMPETTQASGIFTGKADVAWDTTKEGLPQGSITLSGRNVQVTQTVNDAALPVAFQTL
                     NLTAELRNNRAELGWTIRLTNNGQFDGQVQVTDPQGRRNLGGNVNIRNFNLAMINPIF
                     NRGEKAAGMVSANLRLGGDVQSPQLFGQLQVTGVDIDGNFMPFDMQPSQLAVNFNGMR
                     STLAGTVRTQQGEIYLNGDADWSQIENWRARVTAKGSKVRITVPPMVRMDVSPDVVFE
                     ATPNLFTLDGRVDVPWARIVVHDLPESAVGVSSDVVMLNDNLQPEEPKTASIPINSNL
                     IVHVGNNVRIDAFGLKARLTGDLNVVQDKQGLGLNGQINIPEGRFHAYGQDLIVRKGE
                     LLFSGPPDQPYLNIEAIRNPDATEDDVIAGVRVTGLADEPKAEIFSDPAMSQQAALSY
                     LLRGQGLESDQSDSAAMTSMLIGLGVAQSGQIVGKIGETFGVSNLALDTQGVGDSSQV
                     VVSGYVLPGLQVKYGVGIFDSIATLTLRYRLMPKLYLEAVSGVDQALDLLYQFEF"
     gene            15487..15828
                     /gene="ytfP"
                     /locus_tag="ACHOSC_RS06030"
                     /old_locus_tag="pgaptmp_001206"
     CDS             15487..15828
                     /gene="ytfP"
                     /locus_tag="ACHOSC_RS06030"
                     /old_locus_tag="pgaptmp_001206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamylcyclotransferase"
                     /protein_id="HGU4547182.1"
                     /translation="MRIFVYGSLRHKQGNSHWMTNAQLLGDFSIDNYQLYSLGHYPGA
                     VPGNGTVHGEVYRIDNATLAELDALRTRGGEYARQLIQTPYGSAWMYVYQRPVDGLKL
                     IESGDWLDRDK"
     gene            16040..16291
                     /gene="chpS"
                     /locus_tag="ACHOSC_RS06035"
                     /old_locus_tag="pgaptmp_001207"
     CDS             16040..16291
                     /gene="chpS"
                     /locus_tag="ACHOSC_RS06035"
                     /old_locus_tag="pgaptmp_001207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418645.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system antitoxin ChpS"
                     /protein_id="HGU4547183.1"
                     /translation="MRITIKRWGNSAGMVIPNIVMKELNLQPGQSVEAQVSNNQLILT
                     PISRRYSLDELLAQCDMNAAELSEQDVWGKSTPAGDEIW"
     gene            16285..16635
                     /gene="chpB"
                     /locus_tag="ACHOSC_RS06040"
                     /old_locus_tag="pgaptmp_001208"
     CDS             16285..16635
                     /gene="chpB"
                     /locus_tag="ACHOSC_RS06040"
                     /old_locus_tag="pgaptmp_001208"
                     /EC_number="4.6.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endoribonuclease toxin ChpB"
                     /protein_id="HGU4547184.1"
                     /translation="MVKKSEFERGDIVLVGFDPASGHEQQGAGRPALVLSVQAFNQLG
                     MTLVAPITQGGNFARYAGFSVPLHCEEGDVHGVVLVNQVRMMDLRARLAKRIGLAADE
                     VVEEALLRLQAVVE"
     gene            complement(16715..17245)
                     /gene="ppa"
                     /locus_tag="ACHOSC_RS06045"
                     /old_locus_tag="pgaptmp_001209"
     CDS             complement(16715..17245)
                     /gene="ppa"
                     /locus_tag="ACHOSC_RS06045"
                     /old_locus_tag="pgaptmp_001209"
                     /EC_number="3.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001739810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004427 - inorganic diphosphate
                     phosphatase activity [Evidence IEA];
                     GO_process: GO:0006796 - phosphate-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic diphosphatase"
                     /protein_id="HGU4547185.1"
                     /translation="MSLLNVPAGKDLPEDIYVVIEIPANADPIKYEIDKESGALFVDR
                     FMSTAMFYPCNYGYINHTLSLDGDPVDVLVPTPYPLQPGSVIRCRPVGVLKMTDEAGE
                     DAKLVAVPHSKLSKEYDHIKDVNDLPELLKAQIAHFFEHYKDLEKGKWVKVEGWENAE
                     AAKAEIVASFERAKNK"
     gene            17555..18511
                     /gene="ytfQ"
                     /locus_tag="ACHOSC_RS06050"
                     /old_locus_tag="pgaptmp_001210"
     CDS             17555..18511
                     /gene="ytfQ"
                     /locus_tag="ACHOSC_RS06050"
                     /old_locus_tag="pgaptmp_001210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactofuranose ABC transporter
                     substrate-binding protein YtfQ"
                     /protein_id="HGU4547186.1"
                     /translation="MWKRLLVVSAVSAAMSSMALAAPLTVGFSQVGSESGWRAAETNV
                     AKSEAEKRGITLKIADGQQKQENQIKAVRSFVAQGVDAIFIAPVVATGWEPVLKEAKD
                     AEIPVFLLDRSIDVKDKSLYMTTVTADNILEGKLIGDWLVKEVNGKPCNVVELQGTVG
                     ASVAIDRKKGFAEAIKNAPNIKIIRSQSGDFTRSKGKEVMESFIKAENNGKNICMVYA
                     HNDDMVIGAIQAIKEAGLKPGKDILTGSIDGVPDIYKAMIDGEANASVELTPNMAGPA
                     FDALEKYKKDGTMPEKLTLTKSTLYLPDTAKEELEKKKNMGY"
     gene            18651..20153
                     /gene="ytfR"
                     /locus_tag="ACHOSC_RS06055"
                     /old_locus_tag="pgaptmp_001211"
     CDS             18651..20153
                     /gene="ytfR"
                     /locus_tag="ACHOSC_RS06055"
                     /old_locus_tag="pgaptmp_001211"
                     /EC_number="7.5.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0103116 - ABC-type D-galactofuranose
                     transporter [Evidence IEA];
                     GO_process: GO:0042875 - D-galactonate transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="galactofuranose ABC transporter, ATP-binding
                     protein YtfR"
                     /protein_id="HGU4547187.1"
                     /translation="MTTDQHQEILRTEGLSKFFPGVKALDNVDFSLRRGEIMALLGEN
                     GAGKSTLIKALTGVYHADRGTIWLEGQAISPKNTAHAQQLGIGTVYQEVNLLPNMSVA
                     DNLFIGREPKRFGLLRRKEMEKRATELMASYGFSLDVREPLNRFSVAMQQIVAICRAI
                     DLSAKVLILDEPTASLDTQEVELLFGLMRQLRDRGVSLLFVTHFLDQVYQVSDRITVL
                     RNGSFVGCRETRELPQIELVKMMLGRELDTHALQRAGRTLLSDKPVAAFKNYGKKGTI
                     APFDLEVRPGEIVGLAGLLGSGRTETAEVIFGIKPADSGTALIKGKPQTLRSPHQASV
                     LGIGFCPEDRKTDGIIAAASVRENIILALQAQRGWLRPISRKEQQEIAERFIRQLGIR
                     TPSTEQPIEFLSGGNQQKVLLSRWLLTRPQFLILDEPTRGIDVGAHAEIIRLIETLCA
                     DGLALLVISSELEELVGYADRVIIMRDRKQVAEIPLAELSVPAIMNAIAA"
     gene            20167..21189
                     /gene="ytfT"
                     /locus_tag="ACHOSC_RS06060"
                     /old_locus_tag="pgaptmp_001212"
     CDS             20167..21189
                     /gene="ytfT"
                     /locus_tag="ACHOSC_RS06060"
                     /old_locus_tag="pgaptmp_001212"
                     /EC_number="7.5.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313234.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0103116 - ABC-type D-galactofuranose
                     transporter [Evidence IEA];
                     GO_process: GO:0042875 - D-galactonate transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="galactofuranose ABC transporter, ATP-binding
                     protein YtfT"
                     /protein_id="HGU4547188.1"
                     /translation="MPQSLPDTTPPKRRFRWPTGMPQLAALLLVLLVDSLVAPHFWQV
                     VLQDGRLFGSPIDILNRAAPVALLAIGMTLVIATGGIDLSVGAVMAIAGATTAAMTVA
                     GFSLPIVLLSALGTGILAGLWNGILVAILKIQPFVATLILMVAGRGVAQLITAGQIVT
                     FNSPDLSWFGSGSLLFLPTPVIIAVLTLLLFWLLTRKTALGMFIEAVGINIRAAKNAG
                     VNTRIIVMLTYVLSGLCAAIAGIIVAADIRGADANNAGLWLELDAILAVVIGGGSLMG
                     GRFNLLLSVVGALIIQGMNTGILLSGFPPEMNQVVKAVVVLCVLIVQSQRFISLIKGV
                     RSHDKT"
     gene            21176..22171
                     /gene="yjfF"
                     /locus_tag="ACHOSC_RS06065"
                     /old_locus_tag="pgaptmp_001213"
     CDS             21176..22171
                     /gene="yjfF"
                     /locus_tag="ACHOSC_RS06065"
                     /old_locus_tag="pgaptmp_001213"
                     /EC_number="7.5.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418652.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0103116 - ABC-type D-galactofuranose
                     transporter [Evidence IEA];
                     GO_process: GO:0042875 - D-galactonate transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="galactofuranose ABC transporter, permease
                     protein YjfF"
                     /protein_id="HGU4547189.1"
                     /translation="MIKRNLPLMITIGVFVLGYLYCLTQFPGFASTRVICNILTDNAF
                     LGIIAVGMTFVILSGGIDLSVGSVIAFTGVFLAKVIGDFGLSPLLAFPLVLVMGCAFG
                     AFMGLLIDALKIPAFIITLAGMFFLRGVSYLVSEESIPINHPIYDTLSSLAWKIPGGG
                     RLSAMGLLMLAVVVIGIFLAHRTRFGNQVYAIGGNATSANLMGISTRSTTIRIYMLST
                     GLATLAGIVFSIYTQAGYALAGVGVELDAIASVVIGGTLLSGGVGTVLGTLFGVAIQG
                     LIQTYINFDGTLSSWWTKIAIGILLFIFIALQRGLTVLWENRQSSPVTRVNIAQR"
     gene            complement(22204..23202)
                     /gene="fbp"
                     /locus_tag="ACHOSC_RS06070"
                     /old_locus_tag="pgaptmp_001214"
     CDS             complement(22204..23202)
                     /gene="fbp"
                     /locus_tag="ACHOSC_RS06070"
                     /old_locus_tag="pgaptmp_001214"
                     /EC_number="3.1.3.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016233757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042132 - fructose 1,6-bisphosphate
                     1-phosphatase activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class 1 fructose-bisphosphatase"
                     /protein_id="HGU4547190.1"
                     /translation="MKTLGEFIVEKQHEFSHATGELTALLSAIKLGAKIIHRDINKAG
                     LVDILGASGAENVQGEVQQKLDLFANEKLKAALKARDIVAGIASEEEDEIVVFEGCEH
                     AKYVVLMDPLDGSSNIDVNVSVGTIFSIYRRVTPVGTPVTEEDFLQPGNKQVAAGYVV
                     YGSSTMLVYTTGCGVHAFTYDPSLGVFCLCQERMRFPEKGKTYSINEGNYIKFPNGVK
                     KYIKFCQEEDKSTNRPYTSRYIGSLVADFHRNLLKGGIYLYPSTASHPDGKLRLLYEC
                     NPMAFLAEQAGGKASDGKERILDIIPETLHQRRSFFVGNDHMVEDVERFIREFPDA"
     gene            23378..24751
                     /gene="mpl"
                     /locus_tag="ACHOSC_RS06075"
                     /old_locus_tag="pgaptmp_001215"
     CDS             23378..24751
                     /gene="mpl"
                     /locus_tag="ACHOSC_RS06075"
                     /old_locus_tag="pgaptmp_001215"
                     /EC_number="6.3.2.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016881 - acid-amino acid ligase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate:L-alanyl-gamma-D-glutamyl-
                     meso-diaminopimelate ligase"
                     /protein_id="HGU4547191.1"
                     /translation="MRIHILGICGTFMGGLAMLARQLGHEVTGSDANVYPPMSTLLEK
                     QGIELIQGYDASQLDPQPDLVIIGNAMTRGNPCVEAVLEKNIPYMSGPQWLHDFVLRD
                     RWVLAVAGTHGKTTTAGMATWILEQCGYKPGFVIGGVPGNFEVSARLGESDFFVIEAD
                     EYDCAFFDKRSKFVHYCPRTLILNNLEFDHADIFDDLKAIQKQFHHLVRIVPGQGRII
                     WPENDINLKQTMAMGCWSEQELVGEQGHWQAKKLTTDASEWEVLLDGEKVGEVKWSLV
                     GEHNMHNGLMAIAAARHVGVAPADAANALGSFINARRRLELRGEANGVTVYDDFAHHP
                     TAILATLAALRGKVGGTARIIAVLEPRSNTMKMGICKDDLAPSLGRADEVFLLQPAYI
                     PWQVAEVAEACVQPAHWSGDVDTLADMVVKTAQPGDHILVMSNGGFGGIHQKLLDGLA
                     KKAEAAQ"
     gene            complement(24907..25458)
                     /gene="yjgA"
                     /locus_tag="ACHOSC_RS06080"
                     /old_locus_tag="pgaptmp_001216"
     CDS             complement(24907..25458)
                     /gene="yjgA"
                     /locus_tag="ACHOSC_RS06080"
                     /old_locus_tag="pgaptmp_001216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis factor YjgA"
                     /protein_id="HGU4547192.1"
                     /translation="MTKQPEDWLDDVPGDDIEDEDDEIIWVSKSEIKRDAEELKRLGA
                     EIVDLGKNALDKIPLDADLRAAIELAQRIKMEGRRRQLQLIGKMLRQRDVEPIRQALD
                     KLKNRHNQQVVLFHKLENLRDRLIDQGDDAIAEVLNLWHDADRQQLRTLIRNAKKEKE
                     GNKPPKSARQIFQYLRELAENEG"
     gene            25552..26904
                     /gene="pmbA"
                     /locus_tag="ACHOSC_RS06085"
                     /old_locus_tag="pgaptmp_001217"
     CDS             25552..26904
                     /gene="pmbA"
                     /locus_tag="ACHOSC_RS06085"
                     /old_locus_tag="pgaptmp_001217"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloprotease PmbA"
                     /protein_id="HGU4547193.1"
                     /translation="MALAMKVISQVEAQRKILEEAVSTALELASGKSDGAEVAVSKTT
                     GISVSTRYGEVENVEFNSDGALGITVYHQNRKGSASSTDLSPQAIARTVQAALDIARY
                     TSPDPCAGVADKELLAFDAPDLDLFHPAEVSPDEAIELAARAEQAALQADKRITNTEG
                     GSFNSHYGVKVFGNSHGMLQGYCSTRHSLSSCVIAEENGDMERDYAYTIGRAMSDLQT
                     PEWVGADCARRTLSRLSPRKLSTMKAPVIFANEVATGLFGHLVGAIAGGSVYRKSTFL
                     LDSLGKQILPDWLTIEEHPHLLKGLASTPFDSEGVRTERRDIIKDGILTQWLLTSYSA
                     RKLGLKSTGHAGGIHNWRIAGQGLSFEQMLKEMGTGLVVTELMGQGVSAITGDYSRGA
                     AGFWVENGEIQYPVSEITIAGNLKDMWRNIVTVGNDIETRSNIQCGSVLLPEMKIAGQ
                     "
     gene            27087..>27166
                     /locus_tag="ACHOSC_RS06090"
                     /old_locus_tag="pgaptmp_001218"
     CDS             27087..>27166
                     /locus_tag="ACHOSC_RS06090"
                     /old_locus_tag="pgaptmp_001218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313240.2"
                     /note="electron transport protein; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome b562"
                     /protein_id="HGU4547194.1"
                     /translation="MRKSLLAILAVSSLVFSSASFAADLE"
BASE COUNT         6482 a         6838 c         7391 g         6455 t
ORIGIN      
        1 aagttagtaa ccgttgaagg aacttattat tggctgaata atgaattttc ccagttcagt
       61 agatacaaaa gattgtcccg gagtggtgaa taactcaaag catttgaaaa cgcacctgat
      121 tgtgtaaacc aggtgcgttg atcgaacaaa ttactcaccc gccagcgcgc gtgggaacag
      181 tacattgttt tccaggctga tgtgatccat caggtcatca atcagttcat taataccgtt
      241 atacatcgct ttccaggtgg tgcaggcttc cggcggcggt gtgacgttat tggtggtgtg
      301 tttaatcact tccagcagtt cgcccgcttc atcgtgctcg ctttccatca cgctgattgg
      361 tcccattgcc tggctgccca tgccttgttt gatcatcggg aagaggatct gctcttcttt
      421 catcatgtgg ctggaaagct cttcatgcag catagtcagg tattttgtca gcccttttgg
      481 cacgctcggt ttgtcggcgt gaacgcgctc gactttagtc gcttgcagga tcagctccgg
      541 cagctgctcg cggtgacgat cgtggtagcg cacgatgata tggtcaatga tttctgccag
      601 cggggcgcta cgccagtctt tctcaatcgg ttgttcagca agctttgcca gttcagcttc
      661 aatgacctca acatccagtt ctttacgtgc cgccgcgcgc gccagcgtct gcttaccgcc
      721 acagcagtaa tccatatcat atttacgaaa cagagccgaa gcgcgaggga tagagagcgc
      781 cagttcacct aaaggttggt cgcgataagc catagctgat acctcattct taataagata
      841 agatgtattt taaatgcatc tttaaggcaa aagctataac ccttacgtag cagaggctta
      901 tttaactcac tgccagcgtg tcaactttct gagacagatt cagttaaagg tttttctggt
      961 tttgctctga ccgcaatgac cacgccaatc actaacagcg cgataccgct cagcgtcatt
     1021 agcggcggca tttgctggcg gagtaaaaag gtgtacagca aacctgccag cgtttcgaaa
     1081 acaatcagcg gcccgagaat cactgtcggt aatcgctggc tggcgacgtt ccagcagagt
     1141 gcgccaaccc atgagcaaag cacggctatc gcaaccatca gactaataaa caccagcgga
     1201 cgggggccaa aaggtaagga gaagtccggc gtttgtatat tcagccagta acaggcgacg
     1261 agatagccga tgagagaaac cggcagcgtg accagcgcct gcgccgtcgc ccacatcatc
     1321 ggatgtttgt cgggattttc ccgcagccag cgggcgttgc gcagggcata ccatgcccag
     1381 cagaccacgg aaactaacgc tagcacgatg ccagaggtat aacgtgccca gtcaaaatcg
     1441 gggagtccgt ggtttaactc agcaatattc acactcgcca ggccgatgcc aatacaaatc
     1501 agtgccgggg cgagttttcc ccacgcgagt ttgccgtcgc gctggctata aagcagattg
     1561 gcaaagacag gaatgaccac cggcagggtg ccgataatca tcgtggaaac aggcgcgcca
     1621 gtacgttgaa tggcactggc aaggcagaag taataaatga ggttgcccat catagtgagc
     1681 atcaaggcgg taagccagtc ccgacgcgcc aactgacgca gacgcacgcg tcccagccag
     1741 gcaatgggca gcgcaattaa ccctaacgcc agataacgcc ccatcgactg caacatcgcc
     1801 gggtattccg gtacgatcaa cggcccgaca aaaataagcc cccacatcaa ccctgctaac
     1861 agggcgtaca gcacgccgct aatcattact ggcatccatt ggtctgtcag aagaaagttc
     1921 aggaagcaga catgattgct cccggcatcc cgtcattatt agagaggatg cgggaggatg
     1981 ccactttaat taactagtta ttaacattaa aaagatggct tacgctttcg gctaacgttg
     2041 tcgttgggcg gccaatcaat ttgctaagcg ttttgctgtt atcaaacaga ccacctttcg
     2101 atgcgccaac gtcagaatcc gccagcatat ccgccagtcc gtcgggcagt ccgacgcttt
     2161 tcagtgccgc ggcgaaatcg gcttcgctca gattttgata ggtaacctgt ttgccgctct
     2221 gtttggtcag ctctgccgct aactgtgtca atgtccaggc actatcgccc gccagttcgt
     2281 aaaccttgcc ttcgtgaccg gcttcgctaa tcacgcgtgc cgcagctgcc gcataatctg
     2341 cccgcgttgc tgaggcgatt ttgccatcgc ccgccgcacc gataaatacg ccgtgttcca
     2401 gtgctgccgg ggcgctggcg aggtagtttt cgctgtacca gccgttgcgc agcagggtgt
     2461 aaacgatgcc agaatcagcc aacattttct ccgtctcgat gtgctcatcg gcgaggccga
     2521 gcggggaggt atctgcatgt agcaggctgg tataagcgat aaatttcaca ccagccgcct
     2581 ttgcggcatt aataacatta cgatgctgcg gggcacgttg acccacttcg ctggaagaga
     2641 tcagcagtag tttttccact ccctgaagtg cagatgtcag tgcggcttca tcgccgtagt
     2701 cagcctgacg cacggtaatg ccttgtgctg ccagagcctg ggcttttgcc ggattacgaa
     2761 cgatagccac tatttggctg acaggaaccg ttttcttcaa ggattcaata acatagtgac
     2821 caagttggcc agtggcacca gtaatagcga tcatgggaag tctccatcgt tttgtcttgt
     2881 gtttaaatac gctaacacct aaacttactt ttagtaagta cgcacaaaaa ggtaagtatg
     2941 aaatgagtca ggttagcctg tcgcaacaac tgaaagaggg taatctcttt gcggaacagt
     3001 gcccgtcgcg cgaggtgttg aaacacgtca ccagccgttg gggggtgttg attctggtgg
     3061 cgctacgcga aggtactcat cgctttagcg acctgcggcg caaaatcggc ggggtgagcg
     3121 aaaagatgct tgcgcagtcg ttacaggcgt tagaacagga tggttttctt aaccgtatcg
     3181 cgtatccggt ggtgccgccg catgtggaat atagcctcac gccgctgggc gagcaggtga
     3241 gcgaaaaggt tgctgcactg gcggactgga ttgagttgaa tttgcccgag gtgttggcgg
     3301 tgcgggatga acgtgcggca taactgccgg atgcgctgag cttatctggt ctacggtgca
     3361 tggcttgtag ggcggataag atgcgccagc atcgcatccg ccaataaatg ccggatgcgg
     3421 ttgatgattt ataggccgaa aaagaagtgt tttacttact caaatccacc tgataaatcg
     3481 caaacccgat atcatcggtc gcgactttat tcatcggata ttgccctttc tctttaataa
     3541 acgctgcggc tttatctgac ggagaagttt cgaaacggat atccagtttc ttatcgccag
     3601 ctatcggtgc taaacgccag ttgttatctg ccgccgggtg aatttccccc gcacgtttcg
     3661 actcatcagc aatccacgct gccagcaccg agcggttctc atccggtgaa gcaaaagcga
     3721 tatggctgtc gcccgtaccg gcaaatttgc cgccgtaagc gcgatagtta ttggtggcaa
     3781 cgaggaacat ggcgttcgga tcaatcggct tgccattaaa ggtcaggttc ttaatccttt
     3841 ccgcattggc attaatcatc tggcactcgc cgtcataacg ggcaggctgg gtaacatcaa
     3901 tctgataatt cacaccatca atcacatcaa agttataggt gcggaaacca tcccagttga
     3961 tgagtgattg cggtttcgtg ctgttaggat caatctggtt aaactgtccg gcggagcact
     4021 ccagccactc tttcacctct ttaccgctgg ctttcaccac aatcagcgta ttggggtaga
     4081 gataaagatc ggcggcatta cggaaggtca actggccttt ttccacctcc acatagcttg
     4141 ccgggtcatt tttgcgacca ccaactttaa acggtgcggc agctgaaagc accggcagtt
     4201 ttgccagatc cggatcgccc tgaatgtaat gttcgacata cgctttttgc gcgttgttca
     4261 ctacttgcac ggtcggatcg tcctgcacca gcgccagata gctatacata ttgtcggcag
     4321 atttaccgat tggcttgctg acgaactggc gtgtggcatc gtgatcggct ttgagtgttt
     4381 ctaccagctt gctgtcttcc gccgcgaggg attttttatt ggcgatgtcg taaatcggcc
     4441 gagcttccgc tttcgcctgc gtcacctgcc atttaccgct gtcattactg agttgtaagt
     4501 cgaccacccc aagatgatcg ccccacatgc ccggcattac cgccggaaca ccattcagcg
     4561 tgcctttggt gatatcagcc ccttcgatat cagcaaaatc tttacccggg aaaacggcgt
     4621 gagcatgacc aaacataatg gcgttaacgc ccggaatttc actgaggtaa taaactgagt
     4681 tttccgccat tactttatac ggatcggcag acagcccgga atgcgccaga acgacaacga
     4741 catcggcacc tttctcgcgc atttcaggca cgtatttgcg cacggtttcg gtaatatcat
     4801 tcaccgtcac ttttccggat aaattagctt tatcccagcc catgatttgc ggcggcacga
     4861 cgccaatata gccaatcttc agcgtctgtt tttttccgtc tttatcgacc acttcggtat
     4921 ctttaattaa atacggtgta aacattggct gtttggttct ggcgtcaatg acgttggcat
     4981 ttacataagg gaatttcgct cccgccagcg catttttcag gtaatccaga ccgtagttaa
     5041 attcatgatt gccgagtgta ccgaccgtat aatccagcgt attcagcgcc ttataaaccg
     5101 gatgaacatc acctgctttt aatcctttcg ccgacatgta atcggccagc ggactcccct
     5161 gaatcaaatc gccgttatcg accagtacgc tgtttttcac ttcattgcgg gcatcgttaa
     5221 tcaggcttgc cgtacgtacc agtccgaatt tttccgtggc ggtgtctttg taataatcga
     5281 aatccatcat gttgctatgc agatcagtgg tttccatgat acgcagatcg accgtcgctg
     5341 cattcacact ggcggcaatc agcgtggcca ggagcgttgc gctaaactta atcatcaggg
     5401 acatcctttt atcatcggga atacgaaaga aaagggagaa taaacgtctt acttatagaa
     5461 cagtgaagaa tgccacaatt ttacgctttg aaaatgatga cactatcaca gttggcgcat
     5521 tcattaacga tagggtataa gtaaaacaat aagttaacac cgctcacaga gacgaggtgg
     5581 agaaatgtta gatcaagtat gccagcttgc acggaatgca ggcgatgcca ttatgcaggt
     5641 ctacgacggg acgaaaccga tggacgtcgt cagcaaagcg gacaattctc cggtaacggc
     5701 agcggatatt gccgctcaca ccgttatcat ggacggttta cgtacgctga caccggatat
     5761 tccggtcctt tctgaagaag atcctcccgg ttgggaagtc cgtcagcact ggcagcgtta
     5821 ctggctggta gacccgctgg atggtactaa agagtttatt aaacgcaatg gcgaattcac
     5881 cgttaacatt gcgctcattg accatggcaa accgatttta ggtgtggtgt atgcgccggt
     5941 aatgaacgta atgtacagcg cggcagaagg caaggcctgg aaagaagagt gcggtgtgcg
     6001 caagcagatt caggtccgcg atgcgcgccc gccgctggtg gttatcagcc gttcccatgc
     6061 agatgcggag ctgaaagagt atctgcaaca gcttggcgaa catcagacca cgtccatcgg
     6121 ctcttcgctg aaattctgcc tggtggcgga aggacaggcg cagctgtacc cgcgcttcgg
     6181 accaacgaat atttgggaca ccgccgctgg acatgctgtg gccgcagctg ccggagcgca
     6241 cgttcacgac tggcagggta aaccgctgga ttacactccg cgcgagtcgt tcctgaatcc
     6301 tgggttcaga gtgtctattt attaattaat ttatgcagca ggtccatcac ttgctgcacc
     6361 tcttcctgag taagcgcccc gtctttggcc cattgcacgc gaccgtcttt atccagcacc
     6421 actacagcgg aacttttttc atccagctgc caggcaccgc gtgcgacgcc attgctatca
     6481 acaataaact gcgaccaggg ataaagcttt ttattactct ccagactgct gcgcacaaac
     6541 atgccggaac ccggaattgc gtcgtcggtg ttaacaatgg tggtggtctg gtaacgatcg
     6601 tgcggtaact tcgctgattt aatcgcttca atcagcgtcg cgtttttctc ttttgcagag
     6661 gtgcgaccag caatatgttg cagtactcgc acttttccca ctaactgcgc gctgttccag
     6721 gttttgtagc taaactgatc tttatcaagc accaactcgc cacgatcggt gatgccaatc
     6781 ggcggcactc gctgaccggt ttcgaactga tgtgcggaag ccatcatcgg caacagcagg
     6841 caggtgagtg ccagaatctt gcgtagggtc atggtgtttc cttcttatga tatgcaggtg
     6901 atccgaccac ttgggtctga gttttaatca tatgtgctat ttatcgaatt tcccgcaagt
     6961 gtgatgccag tttgcggtca agcgcacaaa tcatatgaaa aatgaatgct tatactgaag
     7021 accgcgcttc ggtaaaaaga taattctgaa taattgtaac ctttaggtaa aaaaagttat
     7081 acgcggtgga aacattgccc ggatagtcta tagtcactag gcattaaaat ttgcgcctca
     7141 taatagttgg gccgattgtg gcaccgcaca ggcgtaatac tcagcaggag ataacaatga
     7201 aaattttcca acggtacaac ccacttcagg tggcgaagta cgtaaagatc ctgttccgtg
     7261 gacggttata catcaaggac gttggcgctt ttgaatttga taagggtaag attcttatcc
     7321 caaaagtgaa ggataagctg catttgtcag tgatgtccga agttaaccgt caggttatgc
     7381 gtctgcaaac agagatggct taaccaaagt gctatgcagt aataagacgg ctcctgattc
     7441 aggagccgtt gatgtttctg ggggttacgc gacgctttct tctttatctt tagcgtcagg
     7501 cagttttggc gaaagggcgg tggccttgct gtcgatccgg gtcaccagca gctggtcgat
     7561 gcggtagtta tcgatatcca ccacttcaaa tttgtagccg gcgaatttca ccgaatcggt
     7621 gcgtttcggg atcttacgca gcataaacat cataaagcct ccgatggttt cgtagttgcc
     7681 cgactgcggg aactcgtcaa tatccagcac gcgcatgacg tcgtcaattg gggtgccgcc
     7741 gtcaatcagc catgagttct catcacgggc gacaatctgc tcttccagcc cctgaccgac
     7801 cagatcgccc atcagcgtgg tcatcacgtc gttgagggtg atgatcccca ccaccagcgc
     7861 gtactcgttc atgatcaccg cgaagtcttc acctgcggtt ttaaaacttt ccaacgcctc
     7921 tgaaagggtt aacgtatccg gcacaatcag cgtgttgcga atttgtacgc cgctgttcag
     7981 tgccaggctt tggttagcca gcacgcggtt cagcaggtct ttagaatcga cataaccgat
     8041 gatgtggtca atatcttcat tacagacgag gaacttagag tgcggatgtt ccgccacctt
     8101 attcttcagg ctttgctcat cttcgtggag atcaaaccaa atcacgtttt cacgcggtgt
     8161 cattgaagac ggaacggtac gggattccag ctcaaagacg ttttcaatca gctcgtgttc
     8221 ctgtttacgt aacacgcccg ccagcgcacc ggcttccact accgcgtaga tgtcatcaga
     8281 agtgatgtca tctttacgta ccattggcag tttgaaaata cggaagatca tgttcgccag
     8341 gccgttgaag aaccacacca gcggggtgca aacgtacagg cagaagcgca tcgggttgat
     8401 gatacgcaaa gccaccgctt ctggcgcaat cataccgatg cgtttcgggg ttaaatccgc
     8461 aaacaggata aacatgccag tcactaacga gaaagagaga ataaagctca gttgctcaga
     8521 gagttctgcc gacatatagc gggagaacag gctgtgaaaa gctggagaaa atgccgcatc
     8581 accgacgata ccgccgagaa tcgcaactgc gttcagaccg atttggacca ccgtaaagaa
     8641 catgccggga ttttcctgca tattcagaac gcgttgggca tttatattgc cttcatcagc
     8701 cagcagttta agtttgattt tgcgtgaggc ggcaagtgag atctcagaca tcgagaagaa
     8761 cgcacttaca gcgatcaagc agagtatgac taaaatactg tttaacatat cttatccgac
     8821 ctttcaggcc ggatcctcgg aaggggaagt tgattaattt gtgtgaaata cacattgaaa
     8881 gccgattcga agaaagtgaa ccggcatttt cagtggctag tatagcgtaa ggtactgtaa
     8941 agtcgccaga gggttaaaat tcagactgct gataaatgca atactgcctg atgcgctatg
     9001 ccgatcaggc ctacgcagtc cctgcaatat attgaatttg gacgattttg taggcctggt
     9061 aaggcattca cgccgcattc ggcataaaca aaacgcactt tatcaacaat ctacatttgt
     9121 acccgtaacg ctatgcttcc ggtggcagac agacgccaat tccgccaatt ccacagtaac
     9181 catacgggtt tttatgcaga tattgctggt ggtcatcttc ggcataataa aacggtgtgg
     9241 cgttagcgat ttccgtggtg atgtgacgat cgtcatcggc ggcaagcatc gccgcctgaa
     9301 aacgttccag actggcgcga gctgcggcat cctgttctgg ggtcagcgga taaatcgctg
     9361 aacgatactg cgtgccgtgg tcattgccct gacgcattcc ctgggcggga tcgtgattct
     9421 cccaaaatac ctgtagcaac tgctcatagc tgatgacaga aggatcgtaa acaatgcgta
     9481 ccgcttcggc atgacccgta tcaccggagc acacttcccg ataagtcgga tttggcgtat
     9541 agccgccggt atagcctgcg gcggtgctgt aaacgccggg taactgccag aacagacgct
     9601 ccacacccca gaaacaaccc atcgcaaaaa tggcaatctc cattccgtca ggtacattgg
     9661 tcattgagtg accgttgacc gcatgcagcg tggctacggg catcggggtg ttacgtccag
     9721 gcagggcatc ggcgggggaa accagatgct ttttatcaaa taaactcatg gtgtcgctct
     9781 cccgaaaatc ggtcattggg gttaaggttg taacaagagg cgtatttgca cacaataacc
     9841 accgtgaata ggtctaaagt aaaacataag aaatatttgg ggtttagtct gctttttaat
     9901 ccatattact ggatttttgt taagccgttt aacggcgttc caggggcagg aaaaaaggat
     9961 attcaggaga aaatgtgcgc tatatccgac agttatgctg tgtaagctta ctctgcttaa
    10021 gcggatctgc cgtcgccgcg aacgtccgtc tacaggtcga ggggttatcg ggacagctgg
    10081 aaaagaacgt tcgtgcgcag ctttctacga ttgaaagtga tgaagtgacg ccagaccgtc
    10141 gctttcgcgc acgcgtcgat gatgccatcc gcgaaggtct gaaagcgctg ggttattacc
    10201 agccgaccat tgaatttgat ctccgtccac cgccaaagaa agggcggcag gtattgatcg
    10261 ccaaagtcac gccaggcgtg ccggtgttaa ttggcggcac cgatgtggta ttgcgcggcg
    10321 gcgcgcggac cgataaagac tatttgaaat tgctcgatac tcgcccggct attggcacgg
    10381 tactgaacca gggcgattat gaaaatttca aaaagtcctt aaccagcatt gcgttgcgta
    10441 aaggttattt cgatagcgaa tttaccaaag cgcagctggg cattgcgctc ggcctgcata
    10501 aagccttctg ggatattgat tataacagtg gcgaacgtta ccgctttggg catgtgacct
    10561 ttgaaggatc acaaatccgc gatgaatacc tgcaaaatct ggtgccgttt aaagagggcg
    10621 atgagtacga atcgaaagat ctggcggaac ttaaccgtcg actttctgct accggctggt
    10681 ttaactcggt cgtagtggct ccacaatttg ataaagcgcg cgaaacgaaa gtattaccct
    10741 tgacgggcgt ggtttcgccg cgaactgaaa acaccatcga aaccggggtc ggttactcta
    10801 cggacgtggg accgcgcgtg aaagctacgt ggaaaaagcc gtggatgaac tcttatggtc
    10861 acagtctgac caccagtacc agtatttccg cgccggaaca gaccctcgac ttcagctata
    10921 aaatgccgct gctgaagaat ccactggaac aatattattt ggtgcagggc ggttttaagc
    10981 gcactgacct gaacgatacc gaatctgact ccactacgct ggtggcttct cgctactggg
    11041 atctctccag cggctggcag cgtgccatta acctgcgctg gagtctcgac cactttactc
    11101 agggtgaaat taccaacacc acgatgctgt tttatcctgg ggtgatgatt agccgcacgc
    11161 gttctcgtgg tggcctgatg ccaacctggg gcgactcgca acgctactct atcgactact
    11221 ccaacacggc ctggggctca gatgtcgatt tctccgtttt ccaggcgcag aacgtctgga
    11281 tccgcacact gtacgatcgc catcgtttcg ttacacgcgg cacgctgggc tggattgaaa
    11341 ccggtgattt cgacaaagta ccgccggatc tgcgtttttt cgccgggggc gatcgcagta
    11401 ttcgcggcta caaatacaaa tctatcgccc cgaaatacgc caacggtgac ctgaaagggg
    11461 cctcgaagtt gataaccgga tcgctggaat accagtacaa cgtgaccgga aaatggtggg
    11521 gcgcggtgtt tgtcgatagt ggcgaagcgg taagcgatat tcgccgcagc gactttaaaa
    11581 ccggtaccgg ggtcggcgtg cgctgggaat cgccggtcgg gccaatcaaa ctcgattttg
    11641 ccgtaccggt cgcggataaa gacgaacacg ggttacagtt ttacatcggt ctggggccag
    11701 aattatgagt ttatggaaaa aaatcagcct cggcgtggtt atcgttatct tactgttgct
    11761 gggatcggtg gcgtttctgg tgggcaccac cagcggcctg catcttgtat ttaaagcggc
    11821 ggatcgctgg gtgccaggac tggatattgg caaggtcacc ggcggctggc gcgatctcac
    11881 cttgtctgac gttcgttatg agcagccagg cgtggcggta aaagcgggta atctgcatct
    11941 ggcggtcggt cttgagtgcc tgtggaacag tagcgtttgt attaatgacc tggcgctgaa
    12001 agacattcag gtcaacatcg acagtaaaaa aatgcctcct tctgaacagg ttgaagaaga
    12061 ggaagatagc ggtccgctgg atctctccac gccgtatccc atcaccctga cacgggtggc
    12121 gctggacaac gtcaacatca agattgatga caccacggtg tcggtgatgg acttcacctc
    12181 cggcctgaac tggcaggaga aaaccctgac cctgaaaccg acgtcgctga aaggcctgct
    12241 gattgctctg ccgaaagtgg cggaagtggc gcaggaagaa gtggtcgaac cgaaaattga
    12301 aaatccgcag ccggaggaaa agccgctcgg cgaaacgctg aaagatctct tttctcgccc
    12361 ggtattgccg gaaatgaccg acttgcattt gccgcttaac ctgaacattg aagagtttaa
    12421 aggcgagcag ttacgcgtga cgggcgacac ggacatcacc gtgcgcacca tgctgctgaa
    12481 agtgagcagc attgacggca atactaaact ggacgccctg gatatcgatt ccagtcaagg
    12541 gagcgtcaac gccagcggca cggcgcagct gtcagacaac tggccggtgg atatcaccct
    12601 caacagcaca ctgaacgtgg agccgttgaa aggtgaaaaa gtgaagctaa aagtgggcgg
    12661 cgcgctgcgc gaacagctgg agattggcgt taatctttcc ggtccggtgg atatggattt
    12721 acgcgcccag acgcgactgg cggaagccgg attgccactc aacgtggaag tgaacagcaa
    12781 acagctttac tggccgttca ccggtgagaa gcagtatcag gcggatgatc tgaaactgaa
    12841 acttaccggc aaaatgaccg attacacgct ctctatgcgt acggcagtga agggactgga
    12901 gatcccgcca gccaccatta cccttgatgc caaaggtaat gaacagcagg tcaatctcga
    12961 caaactcacc gtcgcggcgc tggaagggaa aactgaactc aaggcgttgc tcgactggca
    13021 gcaggccatt agttggcgcg gtgagctaac gcttaacggc attaacaccg ccaaagagat
    13081 cccagagtgg ccgtcgaaac tcaatggctt gattaaaacc cgcggtagcc tgtacggcgg
    13141 cacctggcag atggaggtgc cagaactgaa gctgaccggt aacgtcaaac agaacaaagt
    13201 gaacgttgac ggcacgctga aaggcaacag ttatatgcag tggatgatcc cagggcttca
    13261 tctggaactg gggccaaaca gtgccgaagt gaaaggcgag ctgggggtaa aagatctcaa
    13321 tcttgatgcc accatcaacg cgccggggct ggataacgcg ctgccggggc ttggcggtac
    13381 agcgaaaggg ctggtgaaag ttcgcggcac ggtggaagcg ccacaactac tggcggacat
    13441 caccgcgcgc ggcctgcgct ggcaggagct aaccgtggcg caggttcgcg tggaaggcga
    13501 tatcaaatcc accgttcaga ttgcgggtaa actcgacgtg cgcgttgagc aaatttcaca
    13561 gccggatgta aatatcaacc tcgtcaccct gaatgccaaa ggcagcgaaa aacagcacga
    13621 gttacagttg cggattcagg gcgagccggt ttccgggcag cttaatctgg caggaagttt
    13681 tgatcgcaaa gaagaacgct ggaagggaac tcttagcaat acccgcttcc agacgccggt
    13741 cggcccgtgg tcgctgaccc gcgatattgc gctggattac cgcaataagg agcaaaaaat
    13801 cagcatcggg ccacactgct ggcttaaccc gaatgcggaa ctgtgcgtgc cgcaaactat
    13861 cgatgcgggg gccgaagggc gtgcggtggt gaatctcaac cgcttcgacc tcgccatgct
    13921 gaaaccgttt atgccagaaa ccactcaggc cagcggtatc ttcacgggta aagcggatgt
    13981 tgcctgggac accacgaaag aggggctgcc gcagggcagt atcacccttt cggggcgtaa
    14041 cgtgcaggta acgcaaaccg tcaacgatgc ggcgctgccc gtggcgtttc agacactgaa
    14101 tctgacggcg gaattgcgta acaaccgtgc cgaattgggc tggaccatcc gcctgaccaa
    14161 taacggccag tttgatggac aggtgcaggt gaccgatccg caaggccgcc gtaatcttgg
    14221 tggcaacgtc aatatccgta acttcaacct tgcgatgata aaccccatct ttaaccgtgg
    14281 ggaaaaagca gcggggatgg tgagtgccaa cttgcgtctg ggtggtgatg tgcaaagccc
    14341 gcagttgttt ggtcagcttc aggttacggg tgtggatatc gacggcaact ttatgccgtt
    14401 tgatatgcaa ccgagccagc ttgcggtcaa ctttaatggt atgcgctcga cgcttgccgg
    14461 tacagtacgg acccagcagg gtgaaatcta cctgaacggt gatgccgact ggagccaaat
    14521 tgaaaactgg cgggcgcggg taacggcgaa aggcagtaaa gtgcggatca ccgtgccgcc
    14581 gatggtacga atggatgtat cgccagatgt tgtcttcgag gctacaccaa acctgtttac
    14641 cctcgatggt cgcgtggatg tcccgtgggc gcgcatcgtg gtgcacgatc tgccggaaag
    14701 cgcagtaggc gtctccagcg atgtggtgat gcttaacgat aacctgcaac ctgaagagcc
    14761 gaaaacggcg tcgattccga ttaacagtaa cctgattgtc cacgttggca acaatgtgcg
    14821 cattgacgcc tttggcctga aagcgcggct gacgggcgat cttaacgtcg ttcaggacaa
    14881 acaagggctg ggcctgaacg ggcagatcaa catccctgaa gggcgcttcc atgcctatgg
    14941 tcaggatctg attgtgcgta aaggtgagtt actgttctct ggtccgccgg atcaaccgta
    15001 tcttaatatt gaagctattc gtaacccgga tgctacagaa gacgacgtaa tcgccggagt
    15061 tcgcgtcact ggtctggcgg acgaaccgaa agcggagatc ttctctgacc cggcgatgtc
    15121 gcaacaagct gcattgtctt atttgctacg tggacaaggg ctggagagcg atcagagcga
    15181 cagtgcggca atgacctcga tgctgattgg tctgggggtt gcgcaaagtg gccagattgt
    15241 gggtaaaatc ggcgagacgt ttggcgtaag caatttagcg ctcgacaccc agggagtagg
    15301 cgactcctcc caggtagtgg tcagcggcta tgtattgcca ggtctgcaag tgaaatatgg
    15361 cgtgggtata tttgactcta tagcaacact cacgttacgt tatcgcctga tgcctaagct
    15421 atatctggaa gccgtgtctg gtgtagacca ggcactggat ttgctctatc agttcgagtt
    15481 ttagcaatgc gaatatttgt ctacggcagt ttacgccaca aacaaggcaa cagtcactgg
    15541 atgaccaatg cccagttact gggcgatttc agtatcgata actaccagtt gtatagcctg
    15601 ggccactatc caggcgcagt tccggggaac ggaacagtac acggtgaagt ttatcgtatt
    15661 gacaacgcca cgctggccga acttgatgcc ttgcgcacca ggggcggtga atacgcgcgc
    15721 cagttgattc agacgccgta cgggagtgca tggatgtacg tttatcaacg acccgtcgat
    15781 ggattaaagc taattgaaag cggcgactgg ttagacaggg ataagtaacc atatgcatac
    15841 gccaccttcg ggtggcgttg ttttttgcga gacgactcgc attctgtttt gtaattccct
    15901 caccctttgc ttttctctcc gagccgcttt ccatatctgt taacgcataa aaaactctgc
    15961 tggcgttcac aaatgcgcag gggtaaaacg tttcctgtag caccgtgagt tatactttgt
    16021 ataacttaag gaggtgcaga tgcgtattac cataaaaaga tgggggaaca gtgcaggtat
    16081 ggtcattccc aatatcgtaa tgaaagaact taacttacag ccggggcaga gcgtggaagc
    16141 gcaggtgagc aacaaccaac tgattctgac acccatctcc aggcgctact cgcttgatga
    16201 actgctggca cagtgtgaca tgaacgccgc ggaacttagc gagcaggatg tctggggtaa
    16261 atccacccct gcgggtgacg aaatatggta aagaaaagtg aatttgaacg gggagacatt
    16321 gtgctggttg gctttgatcc agcaagcggc catgaacagc aaggtgctgg tcgacctgcg
    16381 cttgtgctct ccgttcaagc ctttaatcaa ctgggaatga cgctggtggc ccccattacg
    16441 cagggcggaa attttgcccg ttatgccgga tttagcgttc ctttacattg cgaagaaggc
    16501 gatgtgcacg gcgtggtgct ggtgaatcag gtgcggatga tggatctacg cgcccggctg
    16561 gcaaagcgta ttggtctggc tgcggatgag gtggtggaag aggcgttatt acgcttgcag
    16621 gcggtggtgg aataaggtgt gtttatttat cgcgggcata aaaaaaccct tactaaccga
    16681 agctcggcgt tcagggttat tgcgctagaa gaacttattt attctttgcg cgctcgaagg
    16741 aagcaacgat ttcagcttta gcggcttctg cgttttccca accttcaact ttcacccact
    16801 tgcctttttc gaggtctttg tagtgctcga agaagtgagc gatctgcgct ttcagcagct
    16861 ctggcagatc gttaacgtct ttaatgtgat cgtattcttt gctcagcttg ctgtgcggaa
    16921 ccgcaaccag tttcgcatct tcaccggctt cgtcggtcat tttcagaacg ccaaccggac
    16981 ggcaacggat cacagaaccc ggctgcagcg ggtacggagt cgggaccagt acgtcaaccg
    17041 ggtcaccgtc cagagacagg gtgtggttga tgtaaccgta gttgcacgga tagaacatcg
    17101 cggtggacat gaagcggtca acgaacagtg cgccgctctc tttgtcgatt tcgtatttga
    17161 tcggatctgc gttagccggg atctcaataa caacgtagat gtcttccggc agatctttac
    17221 ccgcagggac gttgagtaag ctcatgtctg tttcctttaa aaatatgtgg caaacaagtg
    17281 ccgagtatta tagccaactc gcgccgaatg tctttgcttg ttttcgtctt cattttcctt
    17341 tttcgccagt tttaagacgg tatacagagc aggaaaatcc ataaccgtag ccgcattttt
    17401 catagtgaga tgaaagcgat tacaaacttg tgattaacgt tttatttact tttttgaagt
    17461 gtgatgtaac gcaatccgtt acataacgaa ttgtctatag ttttttcgcg aacatctttt
    17521 caccaataat aattaccccg acgaggacaa ccctatgtgg aaacgcttac ttgtagtctc
    17581 tgcagtctcg gcagccatgt cgtctatggc gttggccgct ccattaaccg taggattttc
    17641 gcaggtcgga tcggaatccg gctggcgcgc tgcagaaacc aatgtggcga aaagtgaggc
    17701 cgaaaaacgc ggaattacgt tgaaaattgc cgatggtcag caaaagcagg aaaaccagat
    17761 taaagcggta cgttccttcg ttgcacaagg ggtggatgcg atctttatcg ctccagtggt
    17821 tgcgacgggt tgggaaccgg tattaaaaga ggcgaaagat gccgaaatcc cggtcttctt
    17881 gcttgaccgt tccatcgatg tgaaagacaa atctctctat atgaccaccg tcactgccga
    17941 caacatcctc gaaggcaagt tgattggtga ctggctggta aaagaagtga atggcaaacc
    18001 atgcaacgtg gtggaactgc agggcaccgt tggggccagc gtcgccattg accgtaagaa
    18061 aggctttgcc gaagccatta agaatgcgcc aaatatcaaa atcatccgct cgcagtcagg
    18121 tgacttcacc cgcagtaaag gcaaagaagt tatggagagc tttatcaaag cggaaaacaa
    18181 cggcaaaaac atctgcatgg tttacgccca taacgatgac atggtgattg gtgcaattca
    18241 ggcaattaaa gaagcgggcc tgaaaccggg caaagatatc ctcacgggtt ccattgacgg
    18301 cgtaccggat atctataaag cgatgattga tggcgaagcg aacgccagcg ttgaactgac
    18361 gccgaacatg gcaggccccg cttttgacgc gctggagaaa tacaaaaaag acggcaccat
    18421 gcctgaaaag ctgaccctga ccaaatccac cctttatctg cctgataccg caaaagaaga
    18481 gttagagaag aagaaaaata tggggtattg agggttgcta tgcctgatgc cgattcgtag
    18541 gccggataag gcgctcgcgc cgcatccggc gatggtgcac tgaagcctga tgcgacgctt
    18601 accgcgtctt atcatgccta ctgggagcac gctttacacc gggggaaacc atgacgaccg
    18661 accaacacca ggagatcctc cgcaccgaag gattaagtaa atttttcccc ggcgtcaaag
    18721 cgttagacaa cgttgatttc agcctgcgcc gtggcgaaat catggcgctg cttggtgaaa
    18781 acggggcggg aaaatcaacg ctaatcaaag cattaactgg tgtataccac gccgatcgcg
    18841 gcaccatctg gctggaaggc caggctatct caccgaaaaa taccgcccat gcacaacaac
    18901 tcggtatcgg caccgtctat caggaagtca acctgctacc caatatgtcg gttgctgata
    18961 atctatttat aggccgcgaa cccaaacgtt tcggccttct acgccgtaaa gagatggaaa
    19021 agcgcgccac cgaactgatg gcatcttacg gtttctccct cgacgtgcgc gaaccgctta
    19081 accgtttttc ggtggcgatg cagcaaatcg tcgccatttg ccgggctatc gatctctccg
    19141 ccaaagtgct gatcctcgat gaacccaccg ccagcctcga cacccaggaa gtggagctat
    19201 tatttggcct gatgcgtcag ttgcgcgatc gcggcgtcag cctgctcttt gtcactcact
    19261 ttctcgatca ggtctatcag gtcagcgatc ggatcaccgt cttacgcaac ggcagtttcg
    19321 taggctgtcg ggaaacgcgc gagctaccgc agatcgaact ggtaaaaatg atgctggggc
    19381 gcgagctgga tacccacgcg ctacagcgtg ccgggcgaac attgttgagc gacaaacccg
    19441 ttgccgcgtt caaaaattac ggcaaaaaag gaacgatagc accgtttgat ctcgaagtgc
    19501 gccccggcga gatcgtcggt ctggctggct tgctgggatc cggacgtacc gaaaccgccg
    19561 aagtgatctt cggtatcaaa cctgctgaca gcggcacggc gttgatcaaa ggcaaaccgc
    19621 aaaccctgcg atcgccacat caggcttcag tactgggcat cggattctgc ccggaagaca
    19681 ggaaaaccga tggcatcatc gctgccgcct cggtgcggga aaatatcatc ctcgctctcc
    19741 aggcccagcg cggctggcta cgacccattt cccgcaaaga gcagcaagag attgccgaac
    19801 gctttatccg ccagcttggc attcgcacac cttcaactga acaaccgatt gaatttctct
    19861 ctggcggcaa tcagcaaaaa gtgttgcttt cacgttggct actgacacga ccgcaatttc
    19921 tgatcctcga cgagccaacc cgcggcattg atgttggtgc ccacgccgag atcatccgcc
    19981 tgattgaaac gctgtgtgct gacggtctgg cgctgttggt gatctcctcc gaactggaag
    20041 agctggtggg ctatgccgat cgggtgatta tcatgcgcga tcgcaaacag gtggcggaga
    20101 tcccgctggc agagctttcc gttccggcga tcatgaacgc cattgcggcg taaggagaac
    20161 agtgtgatgc ctcaatctct cccggacact acgccgccga aaaggcgctt tcgctggcca
    20221 acgggaatgc cgcagctggc ggcgctattg ctggtgctgc tggtcgatag cctggtagcc
    20281 ccgcatttct ggcaggtggt gcttcaggat gggcgtttgt tcggtagccc tatcgacatt
    20341 cttaaccgtg cagctcccgt tgcgttactg gcgattggta tgacgctggt gatcgccacc
    20401 ggtgggattg atctctccgt aggggcggtg atggctatcg ccggagccac aacggctgcg
    20461 atgacggtcg cgggattcag cctgccgatt gttttgttaa gcgccctggg caccggcatc
    20521 ctggcgggat tgtggaacgg catactggta gcgatcctca aaattcagcc gtttgtcgcc
    20581 accctgatcc tgatggtcgc cgggcgcggc gtggcgcaac tgatcaccgc cgggcagatc
    20641 gtcacgttta actcgccgga tctctcatgg tttggcagcg gatcgctgtt gttcctgcca
    20701 acgccggtca ttatcgcggt gctgacgctt ctcctgttct ggctgttgac ccgcaaaacg
    20761 gcgctgggga tgtttatcga agccgttggt atcaacattc gggcggcaaa aaatgccggg
    20821 gtaaacacgc ggatcatcgt catgctcacc tacgtgttga gcgggctgtg tgcggcgatt
    20881 gcgggcatta tcgtggcggc ggatattcgc ggtgccgatg ccaataacgc cgggttatgg
    20941 ctggagctgg acgccattct tgcggtggtg attggcggcg gatcgctgat gggcgggcgc
    21001 tttaacctgc tgctctcagt cgtgggggcg ctgattattc aggggatgaa caccggaatt
    21061 ttactttctg gctttccgcc ggagatgaac caggtcgtaa aagcggtggt ggtgctttgt
    21121 gtgctgattg ttcagtcgca acgctttatc agtctgatta aaggagtacg tagccatgat
    21181 aaaacgtaac ttgccgctga tgatcaccat cggcgtcttt gtgctgggct atctctactg
    21241 cctgacccag tttcccggtt ttgcttccac aagagtgatc tgcaatatcc tgaccgataa
    21301 cgcctttctt gggataatcg ccgttggcat gacctttgtg atcctctctg gcggtatcga
    21361 tctctccgtc ggttcggtga tcgcctttac tggcgtgttt ctggcaaaag tgattggcga
    21421 tttcggcctc tcgccgctgc tggcgtttcc gctagtgctg gtgatgggct gtgcctttgg
    21481 cgcatttatg gggcttttga tcgacgccct gaagatcccg gcatttatca ttaccctggc
    21541 aggaatgttc tttttgcgcg gcgtcagcta tctcgtttca gaagagtcga tcccgataaa
    21601 ccatcccatt tatgacacgc tctcaagcct tgcgtggaaa atccctggcg gcggtcgctt
    21661 aagtgcgatg ggactgctga tgctggcggt ggtggttatc ggcatattcc tcgcgcatcg
    21721 tacccgtttt ggtaatcagg tatacgccat tggcggcaac gcaacgtcgg caaacctgat
    21781 ggggatttct acccgcagca ccactattcg catttatatg ctctccaccg gactggcaac
    21841 gctggcgggg attgtcttct cgatttatac ccaggccgga tatgcgctgg cgggcgtagg
    21901 tgtggaactg gatgctatcg cctcagtggt aattggcggt acgcttttga gcggtggcgt
    21961 tggaacggta ttagggacgc tttttggcgt ggcgattcag gggctaattc agacttatat
    22021 caactttgac ggtacgctga gttcctggtg gacgaaaatc gccatcggca ttttgctgtt
    22081 catttttatt gccttacagc gtggattaac ggtgctgtgg gagaatcgtc agagttcgcc
    22141 agtgacaaga gtcaatattg cgcagcgata aaaacgcctc tccgtgtgga gaggcgcagg
    22201 agattacgcg tccgggaatt cacggataaa gcgttcgaca tcttcaacca tatggtcgtt
    22261 gccgacaaag aacgaacggc gctggtgcag agtttccggg atgatatcca gaatacgctc
    22321 tttgccatcg ctcgctttac cgcccgcttg ttccgccagg aatgccatcg ggttgcactc
    22381 atacagcaaa cgcagtttgc cgtccgggtg gctggcggtg cttgggtaga gataaatacc
    22441 gcctttcagc aggttacggt ggaaatccgc gaccagtgaa ccgatataac gtgaggtata
    22501 cgggcggttg gtggatttat cttcttcctg gcagaattta atgtacttct tcaccccgtt
    22561 cggaaactta atgtagtttc cttcgttgat ggagtaggtt ttgcctttct ccgggaagcg
    22621 catccgttcc tggcacaggc agaaaacgcc cagcgaagga tcgtaagtaa aggcgtgaac
    22681 accgcatccg gtggtgtaaa ccagcatggt agaggagccg tataccacgt aacctgccgc
    22741 aacctgtttg ttaccaggct ggaggaaatc ttcttccgtt accggcgtgc caacaggcgt
    22801 aacgcggcgg tagatggaga aaatggtacc gacagagacg ttaacatcga tgttggacga
    22861 gccatccagg gggtccatca gcaccacgta ttttgcgtgt tcacagcctt caaagacgac
    22921 aatctcatct tcttcttcag aggcaatgcc cgcaacgata tcgcgtgctt tcagtgcggc
    22981 tttcagtttt tcattagcga acaagtcgag tttctgctga acctcgccct gcacgttctc
    23041 agcaccgctg gcacccagga tatcaaccag tcctgctttg ttgatatcgc gatggataat
    23101 cttggcgccc agttttattg ccgacagcaa agcagtgagc tcaccggtag catgagaaaa
    23161 ctcgtgctgc ttttcgacaa taaattcacc taacgttttc ataaaacttt ccctgcaatg
    23221 tttatggagt aaagcgaccg caacaatctt aacaaataat ctcaatgttg cgctcaggtg
    23281 aatcgcgcca gcaaattgcg gattatcctg aaatgcgttt ctcacttgcc cgacatatgc
    23341 gtaaaatgag cggcagatta aaaaaggata gtgacgtatg cgcattcata ttttaggaat
    23401 ttgtggcaca tttatgggcg gtctggcgat gctggcgcgc cagttaggcc atgaagtaac
    23461 gggttcggac gccaatgtgt atccgccgat gagcacctta cttgagaagc aaggcattga
    23521 gctgattcag ggttacgatg ccagccagct cgatccgcag ccggatctgg tgattattgg
    23581 caacgccatg acccgtggaa atccgtgtgt ggaagcggta ctggaaaaaa acatccctta
    23641 tatgtcaggt ccacagtggc tgcacgattt tgtgctgcgc gaccgctggg tgctggccgt
    23701 tgccggtaca cacggcaaaa ctaccaccgc gggaatggcg acctggattc tggaacagtg
    23761 cggttacaaa ccgggatttg tgatcggcgg tgtgccgggg aactttgagg tttcggcgcg
    23821 tctgggcgaa agcgacttct ttgttatcga agcggatgag tatgactgcg ccttcttcga
    23881 caaacgctct aaatttgtcc attactgccc gcgtacgctg atcctcaaca accttgagtt
    23941 cgatcacgcc gatatctttg acgacctgaa agcgatccag aaacagttcc accatctggt
    24001 gcgtatcgtt ccggggcagg gccgtattat ctggccggaa aatgacatca acctgaaaca
    24061 gaccatggcg atgggctgct ggagcgagca ggagctggtg ggtgagcagg gtcactggca
    24121 ggcgaaaaag ctgaccactg acgcttctga atgggaagtc ttgctggatg gcgaaaaagt
    24181 gggcgaagtg aaatggtcgc tggtgggcga acataatatg cacaatggcc tgatggcgat
    24241 tgcggcggct cgccatgttg gtgtagcgcc tgcagatgcc gctaacgcgc tgggttcgtt
    24301 tattaacgct cgtcgccgtc tggagttgcg tggtgaagcg aatggcgtga cggtatatga
    24361 cgattttgcc catcacccga cggcaattct ggcaacgctt gcggcgctgc gtggcaaagt
    24421 tggtggtacg gcgcgcatta ttgctgtgct ggagccgcgc tcgaatacca tgaaaatggg
    24481 gatctgcaaa gacgatctgg caccttcatt aggtcgtgcc gatgaagtct tcctgctgca
    24541 accggcgtat attccgtggc aggtggcaga agtggcagaa gcctgcgttc agcctgcaca
    24601 ctggagtggc gatgtggata cgctggcaga tatggtggtg aaaaccgctc agcctggcga
    24661 ccatattctg gtgatgagca acggcggttt tggtgggatc catcagaaac tgctggatgg
    24721 tctggcgaag aaggcggaag ctgcgcagta attcggcctc agcctgatat agtgtgcgta
    24781 ggccggataa ggcgttcacg ccgcatccgg catttgagca tagtgcctga tgcgacgctt
    24841 gatgcgtctt atcaggccta caggtgcacc gcatccggca aaccatactc acatcaacaa
    24901 cgaaaattac ccttcattct ccgccagttc gcgcagatac tggaaaatct ggcgtgcgga
    24961 tttcggcggc ttattccctt ctttctcttt cttcgcgttg cggatcagag tacgcagctg
    25021 ctgacgatcg gcatcgtgcc acagatttaa tacctcagcg atggcgtcat caccttgatc
    25081 gatcagacga tcgcgcaggt tttccagttt atggaacagc accacctgct ggttgtgacg
    25141 gtttttcagc ttatccagcg cctgacggat cggttctaca tcgcgctggc gcagcatctt
    25201 accgatgagc tgcaactggc ggcggcgacc ttccatctta atacgctggg cgagctcaat
    25261 agccgcgcgc agatccgcat ctaacgggat cttatccagc gcgtttttcc ccagatcaac
    25321 aatttccgcg ccaaggcgtt ttagctcctc ggcatcacgt ttaatttcac ttttactgac
    25381 ccagataatt tcatcgtctt cgtcttcgat gtcatcaccg ggaacgtcgt cgagccagtc
    25441 ttcgggctgc ttagtcatct caggctcctt aaaaaaagag gctaatgtta ccagttaaga
    25501 tgcgcactga aaaacggttc tctgttagac ttcagagaaa ctctctacat tatggcactt
    25561 gcaatgaaag taatctctca agttgaagcg cagcgcaaga ttctggaaga agcagtttcc
    25621 actgcgctgg agttggcctc aggcaaatcg gacggtgcgg aagttgccgt cagcaagacc
    25681 accggcatta gcgtaagcac gcgttatggt gaagtggaga atgttgaatt caatagcgat
    25741 ggcgcgctgg ggatcactgt ttatcaccag aaccgcaaag gtagcgcatc atccaccgat
    25801 ttaagcccgc aggccattgc ccgtactgta caggcggcgc tggatattgc ccgttatacc
    25861 tcgccagatc cctgtgccgg cgtggcagac aaagagctgc tggcctttga cgcaccagat
    25921 ctcgacttgt tccaccctgc ggaagtttcc ccggatgaag ccattgaact ggcggcccgc
    25981 gcagaacagg cggcattgca ggcggacaaa cgcattacca ataccgaagg tggcagcttt
    26041 aacagccact acggtgtcaa agtttttggc aacagccacg gcatgttgca gggttactgc
    26101 tcaacgcgtc attcgctctc cagctgtgta attgccgaag aaaatggcga tatggagcgt
    26161 gattacgcct acaccattgg ccgcgcgatg agcgatctgc aaacgccaga gtgggtcggg
    26221 gccgactgtg ctcgccgtac tttatcgcgc ctgtcaccgc gtaaactctc caccatgaaa
    26281 gcgccagtca tttttgccaa tgaagtggca accgggcttt ttggccatct ggtgggggcg
    26341 atagcgggtg gctcggttta ccgtaaatct acctttctgc tggattcgct gggtaaacaa
    26401 attctgccgg actggctgac cattgaagag catccgcatc tgctgaaagg gctggcgtcg
    26461 acgccattcg acagcgaagg tgtgcgcacc gagcgtcgcg atattattaa agatggcatc
    26521 ctgactcagt ggctgctgac cagctactcg gcgcggaaac tggggctgaa aagcaccgga
    26581 catgcgggcg gtattcacaa ctggcggatt gccggacaag ggctaagctt cgaacaaatg
    26641 ctcaaagaga tgggcaccgg gctggtggtg acggagctga tgggccaggg cgtgagtgcc
    26701 attaccggtg attattcccg tggtgcagcg ggcttctggg tagagaatgg cgaaattcag
    26761 tatcccgtta gtgaaatcac catcgcaggt aatttaaaag atatgtggcg caatattgtc
    26821 accgtcggta acgatattga aacacgcagt aatatacagt gtggttctgt gctgttgccg
    26881 gagatgaaaa tcgccgggca gtaaaaaact ggcgcgacct gccgcgctat aaactaaaaa
    26941 attacacaaa atcattcgca ctgcatcgag acggaaagtg aatgagggcc ggggagcgta
    27001 ctcgcagtac gtgacccggg cgaatgagcg tagccgtcga tgaggcagtg tgaaggatga
    27061 agtgtaaata aaaaaggaag tgagcaatgc gtaaaagcct gttagctatt cttgcagtct
    27121 cctcgttggt attcagttct gcgtcgtttg ccgctgatct tgaaga
//
LOCUS       DAZKHF020000035        26658 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2201S31241
            SAMN43267800-rid25106413.denovo.035, whole genome shotgun sequence.
ACCESSION   DAZKHF020000035 DAZKHF020000000
VERSION     DAZKHF020000035.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267800
            Sequence Read Archive: SRR30307263
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 26658)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 26658)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 26658)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350581.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350581.2
            Genome Coverage       :: 151x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:21:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,652
            CDSs (total)                      :: 5,557
            Genes (coding)                    :: 5,320
            CDSs (with protein)               :: 5,320
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 237
            CDSs (without protein)            :: 237
            Pseudo Genes (ambiguous residues) :: 0 of 237
            Pseudo Genes (frameshifted)       :: 102 of 237
            Pseudo Genes (incomplete)         :: 130 of 237
            Pseudo Genes (internal stop)      :: 52 of 237
            Pseudo Genes (multiple problems)  :: 41 of 237
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26658
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267800-rid25106413.denovo.035"
                     /serotype="O 26:H11"
                     /isolate="2201S31241"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-01"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            <1..708
                     /gene="bcsQ"
                     /locus_tag="ACHOSC_RS06095"
                     /old_locus_tag="pgaptmp_001219"
     CDS             <1..708
                     /gene="bcsQ"
                     /locus_tag="ACHOSC_RS06095"
                     /old_locus_tag="pgaptmp_001219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose biosynthesis protein BcsQ"
                     /protein_id="HGU4547195.1"
                     /translation="TTITAALAWSLQMLGENVLVVDACPDNLLRLSFNVDFTHRQGWA
                     RAMLDGQDWRDAGLRYTSQLDLLPFGQLSIEEQENPQHWQTRLSDICSGLQQLKASGR
                     YQWILIDLPRDASQITHQLLSLCDHSLAIVNVDANCHIRLHQQALPDGAHILINNFRI
                     GSQVQDDIYQLWLQSQRRLLPMLIHRDEAMAECLAAKQPVGEYRSDALAAEEILTLAN
                     WCLLNYSGLKTPVGSAS"
     gene            705..3323
                     /gene="bcsA"
                     /locus_tag="ACHOSC_RS06100"
                     /old_locus_tag="pgaptmp_001220"
     CDS             705..3323
                     /gene="bcsA"
                     /locus_tag="ACHOSC_RS06100"
                     /old_locus_tag="pgaptmp_001220"
                     /EC_number="2.4.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417990.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016760 - cellulose synthase (UDP-forming)
                     activity [Evidence IEA];
                     GO_function: GO:0035438 - cyclic-di-GMP binding [Evidence
                     IEA];
                     GO_process: GO:0006011 - UDP-glucose metabolic process
                     [Evidence IEA];
                     GO_process: GO:0030244 - cellulose biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-forming cellulose synthase catalytic
                     subunit"
                     /protein_id="HGU4547196.1"
                     /translation="MSILTRWLLIPPVNARLIGRYRDYRRHGASAFSATLGCFWMILA
                     WIFIPLEHPRWQRIRAEHKNLYPHINASRPRPLDPVRYLIQTCWLLIGASRKETPKPR
                     RRAFSGLQNIRGRYHQWMNELPERVSHKTQHLDEKKELGHLSAGARRLILGIIVTFSL
                     ILALICVTQPFNPLAQFIFLMLLWGVALIVRRMPGRFSALMLIVLSLTVSCRYIWWRY
                     TSTLNWDDPVSLVCGLILLFAETYAWIVLVLGYFQVVWPLNRQPVPLPKDMSLWPSVD
                     IFVPTYNEDLNVVKNTIYASLGIDWPKDKLNIWILDDGGREEFRQFAQNVGVKYIART
                     THEHAKAGNINNALKYAKGEFVSIFDCDHVPTRSFLQMTMGWFLKEKQLAMMQTPHHF
                     FSPDPFERNLGRFRKTPNEGTLFYGLVQDGNDMWDATFFCGSCAVIRRKPLDEIGGIA
                     VETVTEDAHTSLRLHRRGYTSAYMRIPQAAGLATESLSAHIGQRIRWARGMVQIFRLD
                     NPLTGKGLKFAQRLCYVNAMFHFLSGIPRLIFLTAPLAFLLLHAYIIYAPALMIALFV
                     LPHMIHASLTNSKIQGKYRHSFWSEIYETVLAWYIAPPTLVALINPHKGKFNVTAKGG
                     LVEEEYVDWVISRPYIFLVLLNLVGVAVGIWRYFYGPPTEMLTVVVSMVWVFYNLIVL
                     GGAVAVSVESKQVRRSHRVEMTMPAAIAREDGHLFSCTVQDFSDGGLGIKINGQAQIL
                     EGQKVNLLLKRGQQEYVFPTQVARVMGNEVGLKLMPLTTQQHIDFVQCTFARADTWAL
                     WQDSYPEDKPLESLLDILKLGFRGYRHLAEFAPSSVKGIFRVLTSLVSWVVSFIPRRP
                     ERSETAQPSDQALAQQ"
     gene            3334..5673
                     /gene="bcsB"
                     /locus_tag="ACHOSC_RS06105"
                     /old_locus_tag="pgaptmp_001221"
     CDS             3334..5673
                     /gene="bcsB"
                     /locus_tag="ACHOSC_RS06105"
                     /old_locus_tag="pgaptmp_001221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose biosynthesis cyclic di-GMP-binding
                     regulatory protein BcsB"
                     /protein_id="HGU4547197.1"
                     /translation="MKRKLFWICAVAMGMSAFPSFMTQATPATQPLINAEPAVAAQTE
                     QNPQVGQVMPGVQGADAPVVAQNGPSRDVKLTFAQIAPPPGSMVLRGINPNGSIEFGM
                     RSDEVVTKAMLNLEYTPSPSLLPVQSQLKVYLNDELMGVLPVTKEQLGKKTLAQMPIN
                     PLFITDFNRVRLEFVGHYQDVCENPASTTLWLDVGRSSGLDLTYQTLNVKNDLSHFPV
                     PFFDPRDNRTNTLPMVFAGAPDVGLQQASAIVASWFGSRSGWRGQNFPVLYNQLPDRN
                     AIVFATNDKRPDFLRDHPAVKAPVIEMINHPQNPYVKLLVVFGRDDKDLLQAAKGIAQ
                     GNILFRGESVVVNEVKPLLPRKPYDAPNWVRTDRPVTFGELKTYEEQLQSSGLEPAAI
                     NVSLNLPPDLYLMRSTGIDMDINYRYTMPPVKDSSRMDISLNNQFLQSFNLSSKQEAN
                     RLLLRIPVLQGLLDGKTDVSIPALKLGATNQLRFDFEYMNPMPGGSVDNCITFQPVQN
                     HVVIGDDSTIDFSKYYHFIPMPDLRAFANAGFPFSRMADLSQTITVMPKAPNEAQMET
                     LLNTVGFIGAQTGFPAINLTVTDDGSTIQGKDADIMIIGGIPDKLKDDKQIDLLVQAT
                     ESWVKTPMRQTPFPGIVPDESDRAAETRSTLTSSGAMAAVIGFQSPYNDQRSVIALLA
                     DSPRGYEMLNDAVNDSGKRATMFGSVAVIRESGINSLRVGDVYYVGHLPWFERVWYAL
                     ANHPILLAVLAAISVILLAWVLWRLLRIISRRRLNPDNE"
     gene            5680..6786
                     /gene="bcsZ"
                     /locus_tag="ACHOSC_RS06110"
                     /old_locus_tag="pgaptmp_001222"
     CDS             5680..6786
                     /gene="bcsZ"
                     /locus_tag="ACHOSC_RS06110"
                     /old_locus_tag="pgaptmp_001222"
                     /EC_number="3.2.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose synthase complex periplasmic
                     endoglucanase BcsZ"
                     /protein_id="HGU4547198.1"
                     /translation="MNVLRSGIVTMLLLAAFSVQAACTWPAWEQFKKDYISQEGRVID
                     PSDARKITTSEGQSYGMFFALAANDRAAFDNILDWTQNNLAQGSLKERLPAWLWGKKE
                     NSKWEVLDSNSASDGDIWMAWSLLEAGRLWKEQRYTDIGSALLKRIAREEVVTVPGLG
                     SMLLPGKVGFAEDNSWRFNPSYLPPTLAQYFTRFGAPWTTLRETNQRLLLETAPKGFS
                     PDWVRYEKDKGWQLKAEKTLISSYDAIRVYMWVGMMPDSDPQKARMLNRFKPMATFTE
                     KNGYPPEKVDVATGKAQGKGPVGFSAAMLPFLQNRDAQAVQRQRVADNFPGSDAYYNY
                     VLTLFGQGWDQHRFRFSTKGELLPDWGQECANSH"
     gene            6768..10241
                     /gene="bcsC"
                     /locus_tag="ACHOSC_RS06115"
                     /old_locus_tag="pgaptmp_001223"
     CDS             6768..10241
                     /gene="bcsC"
                     /locus_tag="ACHOSC_RS06115"
                     /old_locus_tag="pgaptmp_001223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026226.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0030244 - cellulose biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose synthase complex outer membrane
                     protein BcsC"
                     /protein_id="HGU4547199.1"
                     /translation="MRKFTLNIFTLSLGLAVMPMVEAAPTAQQQLLEQVRLGEATHRE
                     DLVQQSLYRLELIDPNNPDVVAARFRSLLRQGDIDGAQKQLDRLSQLAPSSNAYKSSR
                     TTMLLSTPDGRQALQQARLQATTGHAEEAVASYNKLFNGAPPEGDIAVEYWSTVAKIP
                     ARRGEAINQLKRINADAPGNTGLQNNLALLLFSSDRRDEGFAVLEQMAKSNAGREGAS
                     KIWYGQIKDMPVSDASVSALKKYLSIFSDGDSVAAAQSQLAEQQKQLADPAFRARAQG
                     LAAVDSGMAGKAIPELQQAVRANPKDSEALGALGQAYSQKGDRANAVANLEKALALDP
                     HSSNNDKWNSLLKVNRYWLAIQQGDAALKANNPDRAERLFQQARNVDNTDSYAVLGLG
                     DVAMARKDYPAAERYYQQTLRMDSGNTNAVRGLANIYRQQSPEKAEAFIASLSASQRR
                     SIDDIERSLQNDRLAQQAEALENQGKWAQAAALQRQRLALDPGSVWITYRLSQDLWQA
                     GQRSQADTLMRNLAQQKPNDPEQVYAYGLYLSGHDQDRAALAHINSLPRAQWNSNIQE
                     LVNRLQSDQVLETANRLRESGKEAEAEAMLRQQPPSTRIDLTLADWAQQRRDYTAARA
                     AYQNVLTLEPTNADAILGLTEVDIAAGDTAAARSQLAKLPATDNASLNTQRRVALAQA
                     QLGDTAAAQQTFNKLIPQAKSQPPSMESAMVLRDGAKFEAQAGDPKQALETYKDAMVA
                     SGVTTTRPQDNDTFTRLTRNDEKDDWLKRGVRSDAADLYRQQDLNVTLEHDYWGSSGT
                     GGYSDLKAHTTMLQVDAPYSDGRMFFRSDFVNMNVGSFSTNADGKWDDNWGTCTLQDC
                     SGNRSQSDSGASVAVGWRNDVWSWDIGTTPMGFNVVDVVGGISYSDDIGPLGYTVNAH
                     RRPISSSLLAFGGQKDSPSNTGKKWGGVRADGVGLSLSYDKGEANGVWASLSGDQLTG
                     KNVEDNWRVRWMTGYYYKVINQNNRRVTIGLNNMIWHYDKDLSGYSLGQGGYYSPQEY
                     LSFAIPVMWRERTENWSWELGASGSWSHSRTKTMPRYPLMNLIPTDWQEEAARQSNDG
                     GSSQGFGYTARALLERRVTSNWFVGTAIDIQQAKDYAPSHFLLYVRYSAAGWQGDMDL
                     PPQPLIPYADW"
     gene            10323..12311
                     /gene="hmsP"
                     /locus_tag="ACHOSC_RS06120"
                     /old_locus_tag="pgaptmp_001224"
     CDS             10323..12311
                     /gene="hmsP"
                     /locus_tag="ACHOSC_RS06120"
                     /old_locus_tag="pgaptmp_001224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417986.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biofilm formation regulator HmsP"
                     /protein_id="HGU4547200.1"
                     /translation="MRVSRSLTIKQMAMVAAVVLVFVFIFCTVLLFHLVQQNRYNTAT
                     QLESIARSVREPLSSAILKGDIPEAEAILASIKPAGVVSRADVVLPNQFQALRKSFIP
                     ERPVPVMVTRLFELPVQISLGVYSLERPANPQPIAYLVLQADSFRMYKFVMSTLSMLV
                     TIYLLLSLILTVAISWCINRLILHPLRNIARELNAIPAQELVGHQLALPRLHQDDEIG
                     MLVRSYNLNQQLLQRHYEEQNENAMRFPVSDLPNKALLMEMLEQVVARKQTTALMIIT
                     CETLRDTAGVLKEAQREILLLTLVEKLKSVLSPRMILAQISGYDFAVIANGVQEPWHA
                     ITLGQQVLTIMSERLPIERIQLRPHCSIGVAMFYGDLTAEQLYSRAISAAFTARHKGK
                     NQIQFFDPQQMEAAQKRLTEESDILNALENHQFAIWLQPQVEMTSGKLVSAEVLLRIQ
                     QPDGSWDLPDGLIDRIECCGLMVTVGHWVLEESCRLLAAWQERGIMLPLSVNLSALQL
                     MHPNMVADMLELLTRYRIQPGTLILEVTESRRIDDPHAAVAILRPLRNAGVRVALDDF
                     GMGYAGLRQLQHMKSLPIDVLKIDKMFVEGLPEDSSMIAAIIMLAQSLNLQMIAEGVE
                     TEAQRDWLAKAGVGIAQGFLFARPLPIEIFEESYLEEK"
     gene            12494..13780
                     /gene="dctA"
                     /locus_tag="ACHOSC_RS06125"
                     /old_locus_tag="pgaptmp_001225"
     CDS             12494..13780
                     /gene="dctA"
                     /locus_tag="ACHOSC_RS06125"
                     /old_locus_tag="pgaptmp_001225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C4-dicarboxylate transporter DctC"
                     /protein_id="HGU4547201.1"
                     /translation="MKTSLFKSLYFQVLTAIAIGILLGHFYPEIGEQMKPLGDGFVKL
                     IKMIIAPVIFCTVVTGIAGMESMKAVGRTGAVALLYFEIVSTIALIIGLIIVNVVQPG
                     AGMNVDPATLDAKAVAVYADQAKDQGIVAFIMDVIPASVIGAFASGNILQVLLFAVLF
                     GFALHRLGSKGQLIFNVIESFSQVIFGIINMIMRLAPIGAFGAMAFTIGKYGVGTLVQ
                     LGQLIICFYITCILFVVLVLGSIAKATGFSIFKFIRYIREELLIVLGTSSSESALPRM
                     LDKMEKLGCRKSVVGLVIPTGYSFNLDGTSIYLTMAAVFIAQATNSQMDIVHQITLLI
                     VLLLSSKGAAGVTGSGFIVLAATLSAVGHLPVAGLALILGIDRFMSEARALTNLVGNG
                     VATIVVAKWVKELDHKKLDDVLNNRAPDGKTHELSS"
     gene            14000..15496
                     /locus_tag="ACHOSC_RS06130"
                     /old_locus_tag="pgaptmp_001226"
     CDS             14000..15496
                     /locus_tag="ACHOSC_RS06130"
                     /old_locus_tag="pgaptmp_001226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M16 family metallopeptidase"
                     /protein_id="HGU4547202.1"
                     /translation="MQGTKIRLLAGGLLMMATAGYVQADALQPDPAWQQGTLSNGLQW
                     QVLTTPQRPSDRVEIRLLVNTGSLAESTQQSGYSHAIPRIALTQSGGLDAAQARSLWQ
                     QGIDPKRPMPPVIVSYDTTLFNLSLPNNRNDLLKEALSYLANATGKLTITPETINHAL
                     QSQDMVATWPADTKEGWWRYRLKGSTLLGHDPADPLKQPVEAEKIKDFYQKWYTPDAM
                     TLLVVGNVDARSVVDQINKTFGELKGKRETPAPVPTLSPLRAEAVSIMTDAVRQDRLS
                     IMWDTPWQPIRESAALLRYWRADLAREALFWHVQQALSASNSKDIGLGFDCRVLYLRA
                     QCAINIESPNDKLNSNLNLVARELAKVRDKGLPEEEFNALVAQKKLELQKLFAAYARA
                     DTDILMGQRMRSLQNQVVDIAPEQYQKLRQDFLNSLTVEMLNQDLRQQLSNDMALILL
                     QPKGEPEFNMKALQAAWDQIMAPSTAAAATSVATDDVHPEVTDIPPAQ"
     gene            complement(15592..16521)
                     /gene="kdgK"
                     /locus_tag="ACHOSC_RS06135"
                     /old_locus_tag="pgaptmp_001227"
     CDS             complement(15592..16521)
                     /gene="kdgK"
                     /locus_tag="ACHOSC_RS06135"
                     /old_locus_tag="pgaptmp_001227"
                     /EC_number="2.7.1.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417983.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydro-3-deoxygluconokinase"
                     /protein_id="HGU4547203.1"
                     /translation="MSKKIAVIGECMIELSEKGADVKRGFGGDTLNTSVYIARQVDPA
                     ALTVHYVTALGTDSFSQQMLDAWHGENVDTSLTQRMENRLPGLYYIETDSTGERTFYY
                     WRNEAAAKFWLESEQSAAICEELANFDYLYLSGISLAILSPTSREKLLSLLRECRANG
                     GKVIFDNNYRPRLWASKEETQQVYQQMLECTDIAFLTLDDEDALWGQQPVEDVIARTH
                     NAGVKEVVVKRGADSCLVSIAGEGLVDVPAVKLPKEKVIDTTAAGDSFSAGYLAVRLT
                     GGSAENAAKRGHLTASTVIQYRGAIIPREAMPA"
     gene            16753..17520
                     /gene="pdeH"
                     /locus_tag="ACHOSC_RS06140"
                     /old_locus_tag="pgaptmp_001228"
     CDS             16753..17520
                     /gene="pdeH"
                     /locus_tag="ACHOSC_RS06140"
                     /old_locus_tag="pgaptmp_001228"
                     /EC_number="3.1.4.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417982.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic-guanylate-specific phosphodiesterase"
                     /protein_id="HGU4547204.1"
                     /translation="MIRQVIQRISNPEASIESLQERRFWLQCERAYTWQPIYQTCGRL
                     MAVELLTVVTHPLNPSQRLPPDRYFTEITVSHRMEVVKEQIDLLAQKADFFIEHGLLA
                     SVNIDGPTLIALRQQPKILRQIERLPWLRFELVEHIRLPKDSTFASMCEFGPLWLDDF
                     GTGMANFSALSEVRYDYIKIARELFVMLRQSPEGRTLFSQLLHLMNRYCRGVIVEGVE
                     TPEEWRDVQNSPAFAAQGWFLSRPAPIETLNTAVLAL"
     gene            17590..19650
                     /locus_tag="ACHOSC_RS06145"
                     /old_locus_tag="pgaptmp_001229"
     CDS             17590..19650
                     /locus_tag="ACHOSC_RS06145"
                     /old_locus_tag="pgaptmp_001229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417981.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AsmA family protein"
                     /protein_id="HGU4547205.1"
                     /translation="MSKAGKITAAISVAFLLLIVVAIILIATFDWNRLKPTINQKVSA
                     ELNRPFAIRGDLGVVWERQKQETGWRSWVPWPHVHAEDIILGNPPDIPEVTMVHLPRV
                     EATLAPLALLTKTVWLPWIKLEKPDARLIRLSEKNNNWTFNLANDDNKDANAKPSAWS
                     FRLDNILFDQGRIAIDDKVSKADLEIFVDPLGKPLPFSEVTGSKGKADKEKVGDYVFG
                     LKAQGRYNGEPLTGTGKIGGMLALRGEGTPFPVQADFRSGNTRVAFDGVVNDPMKMGG
                     VDLRLKFSGDSLGDLYELTGVLLPDTPPFETDGRLVAKIDTEKSSVFDYRGFNGRIGD
                     SDIHGSLVYTTGKPRPKLEGDVESRQLRLADLGPLIGVDSGKGAEKSKRSEQKKGEKS
                     VQPAGKVLPYDRFETDKWDVMDADVRFKGRRIEHGSSLPISDLSTHIILKNADLRLQP
                     LKFGMAGGSIAANIHLEGDKKPMQGRADIQARRLKLKELMPDVELMQKTLGEMNGDAE
                     LRGSGNSVAALLGNSNGNLKLLMNDGLVSRNLMEIVGLNVGNYIVGAIFGDDEVRVNC
                     AAANLDIANGVARPQIFAFDTENALINVTGTASFASEQLDLTIDPESKGIRIITLRSP
                     LYVRGTFKNPQAGVKAGPLIARGAVAAALATLVTPAAALLALISPSEGEANQCRTILS
                     QMKK"
     gene            complement(19884..21206)
                     /locus_tag="ACHOSC_RS06150"
                     /old_locus_tag="pgaptmp_001230"
     CDS             complement(19884..21206)
                     /locus_tag="ACHOSC_RS06150"
                     /old_locus_tag="pgaptmp_001230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HGU4547206.1"
                     /translation="MQATATTLDHEQEYTPINSRNKVLVASLIGTAIEFFDFYIYATA
                     AVIVFPHIFFPQGDPTAATLQSLATFAIAFVARPIGSAVFGHFGDRVGRKATLVASLL
                     TMGISTVVIGLLPGYATIGIFAPLLLALARFGQGLGLGGEWGGAALLATENAPPRKRA
                     LYGSFPQLGAPIGFFFANGTFLLLSWLLTDEQFMSWGWRVPFIFSAVLVIIGLYVRVS
                     LHESPVFEKVAKAKKQVKIPLGTLLTKHVRVTVLGTFIMLATYTLFYIMTVYSMTFST
                     AAAPVGLGLPRNEVLWMLMMAVIGFGVMVPVAGLLADAFGRRKSMVIITTLIILFALF
                     AFNPLLGSGNPILVFAFLLLGLSLMGLTFGPMGALLPELFPTEVRYTGASFSYNVASI
                     LGASVAPYIAAWLQANYGLGAVGLYLAAMAGLTLIALLLTHETRHQSL"
     gene            complement(21617..22630)
                     /gene="yhjD"
                     /locus_tag="ACHOSC_RS06155"
                     /old_locus_tag="pgaptmp_001231"
     CDS             complement(21617..22630)
                     /gene="yhjD"
                     /locus_tag="ACHOSC_RS06155"
                     /old_locus_tag="pgaptmp_001231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inner membrane protein YhjD"
                     /protein_id="HGU4547207.1"
                     /translation="MTQENEIKRPTQDLEHEPIKQLDNSEKGGKVSQALETVTTTAEK
                     VQRQPVIAHLIRATERFNDRLGNQFGAAITYFSFLSMIPILMVSFAAGGFVLASHPML
                     LQDIFDKILQNISDPTLAATLKNTINTAVQQRTTVGLVGLAVALYSGINWMGNLREAI
                     RAQSRDVWERSPQDQEKFWVKYLRDFISLIGLLIALIVTLSITSVAGSAQQMIISALH
                     LNSIEWLKPTWRLIGLAISIFANYLLFFWIFWRLPRHRPRKKALIRGTFLAAIGFEVI
                     KIVMTYTLPSLMKSPSGAAFGSVLGLMAFFYFFARLTLFCAAWIATAEYKDDPRMPGK
                     TQP"
     gene            complement(22679..23578)
                     /locus_tag="ACHOSC_RS06160"
                     /old_locus_tag="pgaptmp_001232"
     CDS             complement(22679..23578)
                     /locus_tag="ACHOSC_RS06160"
                     /old_locus_tag="pgaptmp_001232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417978.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HGU4547208.1"
                     /translation="MDKIHAMQLFIKVAELESFSRAADFFALPKGSVSRQIQALEHQL
                     GTQLLQRTTRRVKLTPEGMTYYQRAKDVLSNLSELDGLFQQDATSISGKLRIDIPPGI
                     AKSLLLPRLSEFLYLHPGIELELSSHDRPVDILHDGFDCVIRTGALPEDGVIARPLGK
                     LTMVNCASPHYLTRFGYPQSPDDLTSHAIVRYTPHLGVHPLGFEVASVNGVQWFKSGG
                     MLTVNSSENYLAAGLAGLGIIQIPRIAVREALRAGRLIEVLPGYRAEPLSLSLVYPQR
                     RELSRRVNLFMQWLAGVMKEHLD"
     gene            24098..24700
                     /locus_tag="ACHOSC_RS06165"
                     /old_locus_tag="pgaptmp_001233"
     CDS             24098..24700
                     /locus_tag="ACHOSC_RS06165"
                     /old_locus_tag="pgaptmp_001233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HGU4547209.1"
                     /translation="MQIVMFDRQSIFIHGMKISLQQRIPGVSIQGASQADELWQKLES
                     YPEALVMLDGDQDGEFCYWLLQKTVVQFPEVKVLITATDCNKRWLQEVIHFNVLAIVP
                     RDSTVETFALAVNSAAMGMMFLPGDWRTTPEKDIKDLKSLSARQREILTMLAAGESNK
                     EIGRALNISTGTVKAHLESLYRRLEVKNRTQAAMMLNISS"
     gene            complement(24751..26400)
                     /locus_tag="ACHOSC_RS06170"
                     /old_locus_tag="pgaptmp_001234"
     CDS             complement(24751..26400)
                     /locus_tag="ACHOSC_RS06170"
                     /old_locus_tag="pgaptmp_001234"
                     /EC_number="3.2.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004555 - alpha,alpha-trehalase activity
                     [Evidence IEA];
                     GO_process: GO:0005993 - trehalose catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha,alpha-trehalase"
                     /protein_id="HGU4547210.1"
                     /translation="MLNQKIQNPNPDELMIEVDLCYELDPYELKLDEMIEAEPEPEMI
                     EGLPASDALTPADRYLELFEHVQSAKIFPDSKTFPDCAPKMDPLDILIRYRKVRRHRD
                     FDLRKFVENHFWLPEVYSSEYVSDPQNSLKEHIDQLWPVLTREPQDHIPWSSLLALPQ
                     SYIVPGGRFSETYYWDSYFTMLGLAESGREDLLKCMADNFAWMIENYGHIPNGNRTYY
                     LSRSQPPVFALMVELFEEDGVRGARRYLDHLKMEYAFWMDGAESLIPNQAYRHVVRMP
                     DGSLLNRYWDDRDTPRDESWLEDVETAKHSGRPPNEVYRDLRAGAASGWDYSSRWLRD
                     TGRLASIRTTQFIPIDLNAFLFKLESAIANISALKGEKETEALFRQKASARRDAVNRY
                     LWDDENGIYRDYDWRREQLALFSAAAIVPLYVGMANHEQADRLANAVRSRLLTPGGIL
                     ASEYETGEQWDKPNGWAPLQWMAIQGFKMYGDDLLGDEIARSWLKTVNQFYLEQHKMI
                     EKYHIADGVPREGGGGEYPLQDGFGWTNGVVRRLIGLYGEP"
BASE COUNT         6206 a         6837 c         7415 g         6200 t
ORIGIN      
        1 acaaccatca ccgccgcatt agcctggtca ttacaaatgt tgggagaaaa tgttctggtg
       61 gtcgatgcct gcccggacaa cttgttgcgc ctgtcattta atgttgattt tacccaccgt
      121 cagggctggg ccagagcgat gctggatggc caggactggc gtgacgctgg gttgcgctac
      181 acctcgcagc tcgatttgct gccttttggt cagttatcca ttgaagaaca agaaaatcca
      241 cagcactggc aaacccggct gagcgatatt tgctccggct tacagcaact aaaagccagc
      301 gggcgttacc agtggatttt aatcgactta ccgcgtgatg cctcgcagat aacccaccag
      361 ctgctgagtt tgtgcgatca ctcgctggct atcgtcaatg tggatgccaa ctgccatatc
      421 cgcctgcatc agcaagcgct gccggatggc gcacatattt tgattaataa cttccgtatt
      481 ggcagtcagg ttcaggacga tatttaccag ctttggttgc aaagccagcg ccgattgctg
      541 ccgatgctca ttcatcgtga tgaagcgatg gctgaatgcc tggcggctaa gcaaccagtc
      601 ggtgaatacc gcagtgatgc gctggcggca gaagagatac tgacgctggc gaactggtgc
      661 ctgttgaact attccgggct gaaaacgcca gtcgggagtg catcatgagt atcctgaccc
      721 ggtggttgct tatcccgccg gtcaacgcgc ggcttatcgg gcgttatcgc gattatcgtc
      781 gccacggtgc gtcggctttc agcgcgacgc tcggctgttt ctggatgatc ctggcctgga
      841 tttttattcc gctggagcac ccgcgctggc agcgtattcg cgcagaacat aaaaacttgt
      901 atccgcatat caacgcctcg cgtccgcgtc cgctggaccc ggtccgttat ctcattcaaa
      961 catgctggtt attgatcggt gcatcgcgca aagaaacgcc gaaaccgcgc aggcgggcat
     1021 tttcaggtct gcaaaatatt cgtggacgtt accatcaatg gatgaacgag ctgcctgagc
     1081 gcgttagcca taaaacacag catctggatg agaaaaaaga gctcggtcat ttgagtgccg
     1141 gggcgcggcg gttgatcctc ggtatcatcg tcaccttctc gctgattctg gcgttaatct
     1201 gcgttactca gccgtttaac ccgctggcgc agtttatctt cctgatgctg ctgtgggggg
     1261 tagcgctgat cgtacggcgg atgccggggc gcttctcggc gctaatgttg attgtgctgt
     1321 cgctgaccgt ttcttgccgt tatatctggt ggcgttacac ctctacgctg aactgggacg
     1381 atccggtcag cctggtgtgc gggcttattc tgctcttcgc tgaaacgtac gcgtggattg
     1441 tgctggtgct cggctacttc caggtagtat ggccgctgaa tcgtcagccg gtgccattgc
     1501 cgaaagatat gtcgctgtgg ccgtcggtgg atatctttgt cccgacttac aacgaagatc
     1561 tcaacgtggt gaaaaatact atttacgcct cgctgggtat cgactggccg aaagacaagc
     1621 tgaacatctg gatcctcgat gacggcggta gggaagagtt tcgccagttt gcgcaaaacg
     1681 tgggggtgaa atatatcgcc cgtaccactc atgaacatgc gaaagcgggc aacatcaaca
     1741 atgcgctgaa atatgccaaa ggcgagttcg tgtcgatttt cgactgcgac cacgtaccaa
     1801 cgcgatcgtt cctgcaaatg accatgggct ggttcctgaa agagaaacag ctggcgatga
     1861 tgcagacgcc gcaccacttc ttctcgccgg acccgtttga acgcaacctg gggcgttttc
     1921 gtaaaacgcc gaacgaaggc acgctgttct atggtctggt gcaggatggc aacgatatgt
     1981 gggacgccac tttcttctgc ggttcctgtg cagtaattcg tcgcaaacca ttggatgaaa
     2041 ttggcggtat tgctgtcgaa acggtaaccg aagatgcgca cacgtctctg cgcctgcacc
     2101 gtcgtggcta cacctctgca tatatgcgta ttccgcaggc ggcggggctg gcgaccgaaa
     2161 gtctgtcggc gcatatcggt cagcgtattc gctgggcacg cgggatggtg caaatcttcc
     2221 gtctcgataa cccgcttacc ggtaaagggc tgaaattcgc ccagcggctg tgctacgtca
     2281 acgccatgtt ccacttcttg tcgggcattc cacgactgat cttcctgact gcgccgctgg
     2341 cgttcctgct gcttcatgcc tacatcatct atgcgccagc gttgatgatc gccctgttcg
     2401 tgctgccgca tatgatccat gccagcctga ccaactccaa gatccagggc aaatatcgcc
     2461 actctttctg gagtgaaatc tacgaaacgg tgctggcgtg gtatatcgca ccaccgacgc
     2521 tggtggcgct gattaacccg cacaaaggca aatttaacgt caccgccaaa ggtggactgg
     2581 tggaagaaga gtacgtcgac tgggtgatct cgcggcccta catcttcctt gtcctgctca
     2641 acctggtggg cgttgcggta ggcatctggc gctacttcta tggcccgcca accgagatgc
     2701 tcaccgtggt cgtcagtatg gtgtgggtat tctacaacct gattgttctt ggcggcgcag
     2761 ttgcagtatc ggtagaaagc aaacaggtac gccgatcgca ccgcgtggag atgacgatgc
     2821 ccgcggcaat tgcccgcgaa gatggtcacc tcttctcgtg taccgttcag gatttctccg
     2881 acggtggttt ggggatcaag atcaacggtc aggcgcagat tctggaaggg cagaaagtga
     2941 atctgttgct taaacgcggt cagcaggaat acgtcttccc gacccaggtg gcgcgcgtga
     3001 tgggtaatga agttgggctg aaattaatgc cgctcaccac ccagcaacat atcgattttg
     3061 tgcagtgtac gtttgcccgt gcggatacat gggcgctctg gcaggacagc tacccggaag
     3121 ataagccgct ggaaagtctg ctggatattc tgaagctcgg cttccgtggc taccgccatc
     3181 tggcggagtt tgcgccttct tcggtgaagg gcatattccg tgtgctgact tctctggttt
     3241 cctgggttgt atcgtttatt ccgcgccgcc cggagcggag cgaaacggca caaccatcgg
     3301 atcaggcttt ggctcaacaa tgatgataac gcgatgaaaa gaaaactatt ctggatttgt
     3361 gcagtggcta tggggatgag tgcgttcccc tctttcatga cgcaggcgac gccagcaacg
     3421 caaccactga tcaatgctga gccagctgta gccgcccaga cggaacaaaa tccgcaggtg
     3481 gggcaagtga tgccgggcgt gcagggcgct gatgcgccag tcgtggcgca gaacggtcct
     3541 tcgcgtgatg tgaagctgac ctttgcgcaa attgcgccgc cgccgggcag catggtgcta
     3601 cgtggcatta acccgaacgg cagcattgag tttggcatgc gcagcgatga agtggtgacg
     3661 aaggcgatgc tcaacctcga atacacgcca tcgccatcgt tactgcctgt ccagtcgcag
     3721 ttaaaggttt atctcaatga tgagctgatg ggcgtgctgc cagtgaccaa agaacagttg
     3781 ggtaaaaaaa cgctggcgca aatgcccatt aacccactgt ttattaccga cttcaaccgt
     3841 gtgcggctgg agtttgtcgg ccattatcag gacgtgtgcg aaaacccggc cagcaccacg
     3901 ctttggctgg atgttgggcg gagcagtgga ctggatctga cctatcagac cctgaatgtg
     3961 aagaatgacc tgtcacactt cccggtgcca ttctttgacc cgcgcgataa ccgtactaac
     4021 accttgccga tggtctttgc gggtgcgccg gatgttgggc tgcaacaagc ctctgccatt
     4081 gtcgcctcgt ggtttggttc gcgttctggc tggcgtgggc agaacttccc ggtgctctat
     4141 aaccaactgc cggatcgtaa tgccattgtc tttgcaacca acgacaaacg gccggacttc
     4201 ctgcgcgatc atccggcggt aaaagcccct gtgattgaga tgattaacca tccgcagaat
     4261 ccttacgtca aactgctggt ggtgtttggt cgtgacgaca aagacctgtt gcaggcagcg
     4321 aaaggtatcg ctcagggtaa cattctgttc cgtggtgaaa gcgtggtagt gaatgaagtg
     4381 aaaccgctgc taccgcgtaa gccgtacgat gcgccgaact gggtacgtac cgatcgtccg
     4441 gtcacatttg gcgaactgaa aacctatgaa gaacagttac aatccagcgg tcttgagcca
     4501 gcagcgatta acgtttcgct aaacctgccg ccggatctct acctgatgcg cagtaccggc
     4561 attgatatgg atattaatta ccgctacacc atgccgccgg tgaaagacag ttcgcggatg
     4621 gatatcagcc tgaataacca gttcctgcaa tccttcaatc tgagcagcaa acaggaggcg
     4681 aaccgcctgc tgctgcggat tccggtatta caaggtttgc tggatggcaa aacagatgtc
     4741 tctattccgg cgctgaaact gggcgcgacc aaccagctgc gcttcgactt tgagtatatg
     4801 aacccgatgc cgggcggttc ggtggataac tgtattacct tccagccggt gcagaatcat
     4861 gtggtgattg gtgacgactc caccatcgac ttctcgaagt attaccactt catcccgatg
     4921 ccggatctac gcgcctttgc taacgcgggc ttcccattca gccggatggc ggatctgtcg
     4981 caaaccatca ccgtgatgcc gaaagcgcct aacgaagcac agatggaaac gttgctgaat
     5041 actgttggtt ttatcggcgc acagacgggc ttcccggcga ttaatctgac ggtgaccgat
     5101 gatggcagca ccattcaggg caaagatgcc gacatcatga tcatcggtgg tatcccggac
     5161 aaactgaaag acgataagca gatcgaccta ttggtgcagg cgaccgaaag ctgggtgaaa
     5221 acaccgatgc gccagacccc gttccccggc attgtgccgg acgagagcga tcgcgcggca
     5281 gaaacccggt caacgctgac ctcttccggt gcgatggcgg cggtgattgg cttccagtcg
     5341 ccgtataacg accagcgcag cgtgattgcg ctgttggcag atagcccacg cggttatgaa
     5401 atgcttaacg atgcggtgaa cgatagcggc aaacgcgcca ccatgttcgg ttcggtcgcg
     5461 gtgatccgcg agtccggtat caacagccta cgtgttggcg acgtttatta cgtaggtcat
     5521 ctgccgtggt tcgagcgcgt gtggtatgcg ctggcaaacc atccgattct gctggcggtg
     5581 ctggcggcta tcagtgtgat attgctggca tgggtactgt ggcgtctgct gcgaattatt
     5641 agtcgtcgtc gtcttaaccc ggataacgag taattgaaga tgaatgtgtt gcgtagtgga
     5701 atcgtgacga tgctgctgct ggctgccttt agtgttcagg cagcctgtac ctggcctgcc
     5761 tgggagcagt ttaaaaagga ttacatcagt caggaagggc gcgtcatcga ccccagcgac
     5821 gcgcgcaaaa tcaccacctc cgaagggcaa agttacggca tgttctttgc cctggcggct
     5881 aacgaccgtg cagctttcga taatattctc gactggacgc agaacaatct cgctcagggt
     5941 tctttaaaag aacgtttgcc cgcctggctg tggggcaaga aagagaacag taagtgggaa
     6001 gtgctggaca gcaattcggc ctccgatggt gatatctgga tggcctggtc gttgctggag
     6061 gcggggcgtt tgtggaaaga gcagcgttat accgacatcg gcagcgcgtt gctaaaacgt
     6121 atcgcgcggg aggaagtggt gacggtgcct gggctgggtt ccatgttgtt accgggcaaa
     6181 gtgggttttg ctgaggataa cagctggcgt tttaacccca gctacctgcc gccgacgctg
     6241 gcgcagtatt tcacccgctt tggcgcgccg tggaccacgc tgcgcgaaac caatcaacgt
     6301 ttattgctgg aaaccgcccc gaaaggtttt tcgccagact gggtgcgcta tgagaaagac
     6361 aaaggctggc agctaaaagc cgaaaaaaca ttgatcagca gctacgacgc tatccgcgtt
     6421 tacatgtggg taggcatgat gcctgacagc gatccgcaaa aagcgcggat gctcaaccgg
     6481 tttaaaccga tggcgacatt cactgagaaa aacggttatc cgccggaaaa agtggatgtg
     6541 gctacgggga aagcgcaggg taaaggaccg gtcggttttt ctgccgccat gctgcccttt
     6601 ttacaaaacc gcgatgcgca ggccgttcag cgccagcgcg tggccgataa ctttcccggc
     6661 agcgatgcct attacaacta tgtgctgacc ctgtttggac aaggctggga tcaacaccgt
     6721 ttccgcttct cgacaaaagg tgagttatta cctgactggg gccaggaatg cgcaaattca
     6781 cactaaacat attcacgctt tccctcggtc tggccgtcat gccgatggtc gaggcagcac
     6841 caaccgctca gcaacagttg ctggagcaag ttcggttagg cgaagcgacc catcgtgaag
     6901 atctggtgca acagtcgtta tatcggctgg aacttattga tccgaataac ccggacgtcg
     6961 ttgccgcccg tttccgttct ttgttacgtc agggcgatat tgatggcgcg caaaaacagc
     7021 tcgatcggct gtcgcagtta gcgccgagtt caaatgcgta taaatcgtcg cggactacga
     7081 tgctactttc cacgccggat ggtcgtcagg cactgcaaca ggcacgattg caggcgacga
     7141 ccggtcatgc agaagaagct gtggcgagtt acaacaaact gttcaacggt gcgccgccgg
     7201 aaggtgacat tgctgtcgag tactggagta cggtggcgaa aattccggct cgccgtggcg
     7261 aagcgattaa tcagttaaaa cgcatcaatg cggatgcacc gggcaatacg ggcctgcaaa
     7321 acaatctggc gctattgctg tttagtagcg atcgccgtga cgaaggtttt gccgtcctgg
     7381 aacagatggc aaaatcgaac gccgggcgcg aaggggcctc taaaatctgg tacgggcaga
     7441 ttaaagacat gcccgtcagt gatgccagtg tgtcggcgct gaaaaaatat ctctcgatct
     7501 ttagtgatgg cgatagcgtg gcggctgcgc aatcgcaact ggcagaacag caaaaacagc
     7561 tggccgatcc tgctttccgc gctcgtgcgc aaggtttagc ggcggtggac tctggtatgg
     7621 cgggtaaagc cattcccgaa ctacaacagg cggtgcgggc gaacccgaaa gacagtgaag
     7681 ctctgggggc gctgggccag gcgtattctc agaaaggcga tcgcgccaat gcagtggcga
     7741 atctggaaaa agccctcgca ctggacccgc acagcagcaa caacgacaaa tggaacagtc
     7801 tgctgaaagt aaaccgctac tggctggcga tccagcaggg cgatgctgcg ctgaaagcca
     7861 ataatcctga ccgggcagaa cgcctgttcc agcaggcgcg taatgtcgat aacaccgaca
     7921 gttatgcagt gctggggctg ggcgatgtgg cgatggcgcg aaaagattat cccgccgccg
     7981 aacgttatta tcagcagacc ttgcgtatgg acagcggcaa cactaacgcc gtgcgcgggc
     8041 tggcaaatat ttaccgccag caatcgccag aaaaagctga agcgtttatc gcctcgctct
     8101 ctgccagtca gcggcgtagc attgatgata tcgaacgcag cctgcaaaac gaccgtctgg
     8161 cacagcaggc agaggcactg gaaaaccagg gcaaatgggc gcaggcggca gcacttcagc
     8221 ggcaacgact ggcgctggac cccggcagcg tatggattac ttaccgactt tcgcaggatc
     8281 tctggcaggc cggacaacgc agccaggccg atacgttaat gcgcaatctg gcgcagcaga
     8341 agccgaacga cccggagcag gtttacgctt acgggctgta cctctctggt catgaccagg
     8401 acagagcggc gctggcgcat atcaacagcc tgccgcgcgc gcagtggaac agcaatattc
     8461 aggagctggt taatcgactg caaagcgatc aggtgctgga aaccgctaat cgcctgcgag
     8521 aaagcggcaa agaagctgaa gcggaagcga tgctgcgtca gcaaccacct tccacgcgta
     8581 ttgacctcac gctggctgac tgggcgcagc agcgacgtga ttacaccgcc gcccgcgctg
     8641 catatcagaa tgtcctgacg ctggagccaa ctaacgccga tgccattctg gggctgacgg
     8701 aagtggatat tgctgccggt gacacagcgg cggcacgtag ccagctggcg aaactgcccg
     8761 ctaccgataa cgcctcgctg aacacacagc ggcgcgtggc gctggcgcag gcgcagcttg
     8821 gagataccgc agcggcgcag cagacgttta ataagttgat cccgcaggca aaatctcagc
     8881 caccgtcgat ggaaagcgcg atggtgctgc gcgacggtgc gaagtttgaa gcgcaggcgg
     8941 gcgatccaaa gcaggcgctg gaaacctaca aagacgccat ggtcgcatcc ggtgtgacta
     9001 cgacgcgtcc gcaggataac gacacattca cccgactgac ccgtaacgac gagaaagatg
     9061 actggctgaa acgcggcgtg cgcagcgatg cggcggacct ctatcgccag caggatctta
     9121 acgtcaccct cgagcacgat tactggggtt cgagcggcac cggtggttac tccgatctga
     9181 aagcgcacac taccatgttg caggtggatg cgccgtattc tgacgggcgg atgttctttc
     9241 gcagtgattt cgtcaatatg aacgtcggca gtttctccac taatgccgat ggcaaatggg
     9301 atgacaactg gggcacctgt acattacagg actgtagcgg caaccgcagc cagtcggatt
     9361 ccggtgccag cgtggcggtc ggctggcgaa atgacgtctg gagctgggat atcggtacca
     9421 cgccgatggg cttcaacgtg gtggatgtgg tcggcggcat cagttacagc gatgatatcg
     9481 ggccgctggg ttacaccgtt aacgcccacc gtcggcccat ctccagttct ttgctggcct
     9541 ttggtgggca aaaagactcc ccgagcaata ccgggaaaaa atggggtggc gtacgtgccg
     9601 acggtgtggg gctaagtctg agctacgata aaggtgaagc aaacggcgtc tgggcatcgc
     9661 ttagtggcga ccagttaacc ggtaaaaatg tcgaagataa ctggcgcgtg cgctggatga
     9721 cgggctatta ctataaggtc attaaccaga acaatcgccg cgtcacaatc ggcctgaaca
     9781 acatgatctg gcattacgac aaagatctga gtggctactc actcggtcag ggcggttact
     9841 acagtccgca ggaatacctg tcgtttgcca taccggtgat gtggcgggag cgcacggaaa
     9901 actggtcgtg ggagctgggg gcgtctggct cgtggtcgca ttcacgcacc aaaaccatgc
     9961 cgcgttatcc gctgatgaat ctgatcccga ccgactggca ggaagaagct gcgcggcaat
    10021 ccaacgatgg cggcagcagt cagggcttcg gctacacggc gcgggcatta cttgaacgac
    10081 gtgttacttc caactggttt gttggcacgg caattgatat ccagcaggcg aaagattacg
    10141 cacccagcca tttcctgctc tacgtacgtt attccgccgc cggatggcag ggtgacatgg
    10201 atttaccgcc gcagccgctg ataccttacg ccgactggta agttttcaga tagcgcctct
    10261 cttaatgccg ctgcgatcgg gtatactcgg gcggcaatct gggatttccg gggggagaca
    10321 atttgcgcgt aagtcgctcg ttaacaatca agcagatggc aatggtggca gccgttgtcc
    10381 tggtgttcgt ttttattttt tgcaccgttt tgctgttcca tctggtccaa cagaatcgct
    10441 ataacacggc tacgcaactg gaaagcattg ctcgctctgt ccgcgaaccc ttatcttcag
    10501 ctattttgaa aggcgatatt cccgaagcgg aagctattct tgccagcatt aaaccggcag
    10561 gcgtggtcag ccgtgccgat gtagtgctgc ctaaccagtt ccaggcgctg cgtaaaagtt
    10621 ttattccaga gcgcccggtg ccggtaatgg ttactcgcct gtttgagcta ccggttcaaa
    10681 tctcgctggg cgtttactcg ctcgaacgtc cggcaaaccc gcagccaatt gcctatctgg
    10741 tactacaggc ggattccttc cgtatgtata agttcgtgat gagcaccctc tcaatgttag
    10801 tgaccattta cttacttttg tcgcttatcc tgaccgtcgc catcagctgg tgcattaacc
    10861 gcctgatttt gcatccgtta cgcaatattg ctcgcgaact taacgccatc ccagcccagg
    10921 agcttgttgg tcaccaactg gcattaccgc gtctgcatca ggacgatgaa atcggtatgt
    10981 tggtgcgcag ttacaacctc aaccagcaat tgctgcagcg ccattatgaa gaacagaacg
    11041 aaaatgcgat gcgcttcccg gtgtcggatt tgccgaacaa agccttgctg atggagatgc
    11101 tggagcaggt tgtcgcgcgt aaacaaacca ccgcgctgat gatcatcacc tgtgaaaccc
    11161 tgcgtgatac tgcgggcgtg ctgaaagagg cgcaacgaga aattctgctg ctgacgctgg
    11221 tggaaaaact caaatcggta ctgtcgccac gtatgatcct cgcgcagatt agcggttatg
    11281 actttgctgt cattgccaac ggtgtacagg aaccgtggca cgcaatcacc ttaggtcagc
    11341 aagtgctcac tatcatgagc gagcgcctgc cgattgaacg tattcaactc cgtccgcact
    11401 gtagcattgg cgtggcgatg ttctacggcg atctcaccgc cgaacagctt tacagtcgcg
    11461 ctatttctgc ggcatttacc gctcgccata aaggcaagaa tcagattcag ttctttgatc
    11521 cgcagcagat ggaagccgcc cagaagcggt tgacggaaga gagcgatatc cttaatgcac
    11581 tggaaaatca tcagtttgct atttggttac agccacaggt cgagatgacc agcggaaaac
    11641 tggtcagtgc ggaagtgtta ctgcgtatcc agcaaccgga tggcagttgg gacctgccgg
    11701 atggcttaat cgatcgcatt gagtgctgtg ggctgatggt taccgtcggt cactgggtgc
    11761 tggaagagtc ctgtcgattg cttgcagcct ggcaagagcg cggcattatg ctgcccttgt
    11821 cggtaaacct ctctgcgctg caactgatgc acccaaatat ggtggcggat atgctggaac
    11881 tgttaacccg ctatcgcatt cagccgggaa cactgattct ggaagtgaca gaaagccgac
    11941 gtattgacga ccctcatgct gcggtggcaa tcctccgtcc gctgcgcaat gccggagttc
    12001 gggtggcgct ggatgatttc ggcatgggct acgcagggct gcgtcagctg cagcatatga
    12061 aatcgttgcc aatcgacgta ctgaaaatcg acaaaatgtt tgttgaaggc ttgccggaag
    12121 atagcagcat gattgctgca attatcatgc tggcgcagag cctgaactta caaatgattg
    12181 ccgaaggcgt ggagactgaa gcacaacgcg actggctggc aaaagcgggc gttggtattg
    12241 cccagggctt cctttttgct cgcccactcc ctattgaaat cttcgaagag agttacctgg
    12301 aagaaaagta gctaccccaa actgattaca aaactttaaa aagtgctggt ttgtgcgagc
    12361 cagctcaaac tttttaacct ttttgtttca attatgatcc aggtacattt ctgtgatgtt
    12421 gtctgggtgt tattttaagg ccgcaggtac cccataacct tacaagacct gtggttttac
    12481 taaaggacac cctatgaaaa cctctctgtt taaaagcctt tactttcagg tcctgacagc
    12541 gatagccatt ggtattctcc ttggccattt ctatcctgaa ataggcgagc aaatgaaacc
    12601 gcttggcgac ggcttcgtta agctcattaa gatgatcatc gctcctgtca tcttttgtac
    12661 cgtcgtaacg ggcattgcgg gcatggaaag catgaaggcg gtcggtcgta ccggcgcagt
    12721 cgcactgctt tactttgaaa ttgtcagtac catcgcgctg attattggtc ttatcatcgt
    12781 taacgtcgtg cagcctggtg ccggaatgaa cgtcgatccg gcaacgcttg atgcgaaagc
    12841 ggtagcggtt tacgccgatc aggcgaaaga ccagggcatt gtcgccttca ttatggatgt
    12901 catcccggcg agcgtcattg gcgcatttgc cagcggtaac attctgcagg tgctgctgtt
    12961 tgccgtactg tttggttttg cgctccaccg tctgggcagc aaaggccaac tgatttttaa
    13021 cgtcatcgaa agtttctcgc aggtcatctt cggcatcatc aatatgatca tgcgtctggc
    13081 gcctattggt gcgttcgggg caatggcgtt taccatcggt aaatacggcg tcggcacact
    13141 ggtgcaactg gggcagctga ttatctgttt ctacattacc tgtatcctgt ttgtggtgct
    13201 ggtattgggt tcaatcgcta aagcgactgg tttcagtatc ttcaaattta tccgctacat
    13261 ccgtgaagaa ctgctgattg tactggggac ttcatcttcc gagtcggcgc tgccgcgtat
    13321 gctcgacaag atggagaaac tcggctgccg caaatcggtg gtggggctgg tcatcccgac
    13381 aggctactcg tttaaccttg atggcacatc gatatacctg acaatggcgg cggtgtttat
    13441 cgcccaggcc actaacagtc agatggatat cgtccaccaa atcacgctgt taatcgtgtt
    13501 gctgctttct tctaaagggg cggcaggggt aacgggtagt ggctttatcg tgctggcggc
    13561 gacgctctct gcggtgggcc atttgccggt agcgggtctg gcgctgatcc tcggtatcga
    13621 ccgctttatg tcagaagctc gtgcgctgac taacctggtc ggtaacggcg tagcgaccat
    13681 tgtcgttgct aagtgggtga aagaactgga ccacaaaaaa ctggacgatg tgctgaataa
    13741 tcgtgcgccg gatggcaaaa cgcacgaatt atcctcttaa tctcatcact tatgcccgca
    13801 ctcccttccc cgagtgcggg tgtttgcgca taattgaccc ctgtgcctga acttaccacc
    13861 tgggtgagtt caggtttatt tcacttcttc cgtgacattt tcatgttctt gcggtctaac
    13921 acgaagtgtt tttaacgtca tttagactga ccgacaacgg gcagtttttt attaccagga
    13981 tagttgatca ggggttcaca tgcagggcac aaaaattcga cttttagcgg gcggtttgct
    14041 gatgatggcc actgctggct atgtgcaggc agatgcgctc cagcctgatc cagcatggca
    14101 acaggggacg ctttccaacg gtttacagtg gcaagtgctg accacccccc agcgtcccag
    14161 cgatcgtgtt gaaattcgcc tgctggttaa taccggttcg ctcgccgaaa gtacacaaca
    14221 gagcggttac agtcacgcca tccctcgtat tgcgctaacg caaagcggtg gccttgacgc
    14281 agcacaggcg cgttcattgt ggcagcaggg gatcgaccct aaacgcccga tgccgccggt
    14341 aattgtctct tatgacacca cgctgtttaa tctgagtttg cccaataacc gtaacgattt
    14401 gctgaaagaa gcgctctctt atctggcaaa tgccactggc aaattgacca tcacaccaga
    14461 aaccatcaac cacgcgctgc aaagtcagga catggtggca acctggcctg ccgatactaa
    14521 agagggctgg tggcgctatc gtctgaaagg gtcaaccttg ttaggtcacg atcctgccga
    14581 tccgctgaaa caacccgttg aagcggaaaa aattaaagat ttctatcaga aatggtacac
    14641 cccggatgca atgacgctac tggtggtggg aaacgtggat gcgcgctcgg ttgtcgacca
    14701 aataaacaaa acgtttggcg aactgaaagg caaacgtgaa acgccagctc cggtgccgac
    14761 gctttctccg ctgcgtgcgg aagcggtgag tattatgact gacgcggtgc gtcaggaccg
    14821 gttatctatc atgtgggata cgccgtggca gccgattcgt gaatcagccg cactgctgcg
    14881 ctactggcgt gcggacctgg cccgcgaggc gctgttctgg catgttcagc aagcgttaag
    14941 cgccagtaac agcaaagaca tcggtcttgg atttgactgc cgtgtgctgt atctgcgtgc
    15001 gcagtgtgcc atcaacatcg aatcaccaaa cgacaagctg aacagcaacc ttaatctggt
    15061 ggcgcgtgaa ctggcgaagg ttcgcgataa aggtctgccg gaagaagagt tcaatgcgtt
    15121 agtggcgcaa aagaaactgg agctgcagaa actgtttgcc gcctatgcgc gggctgatac
    15181 cgatattctg atgggtcagc ggatgcgttc gttgcaaaat caggtcgtcg atatcgcgcc
    15241 ggagcagtat cagaaactgc ggcaggattt ccttaacagc ctgacggtgg agatgttaaa
    15301 tcaggatctg cgtcagcagt tgtcgaatga tatggcgtta atactgctgc agccgaaagg
    15361 cgagccggaa tttaacatga aagcgttgca ggcggcctgg gatcaaatca tggccccatc
    15421 gactgctgct gccgccacct ctgtcgccac ggatgacgta catcctgaag tgacggatat
    15481 tccacctgca cagtaatgcc tgatggtgat gagttagcac aaccatcagg tatttatgga
    15541 tgagctggat agaggggtaa cactttatcc agccttttgc atatgctgcg tttacgctgg
    15601 catcgcctca cgcgggataa tcgcgccgcg atactgaata acggtacttg cggtcaggtg
    15661 cccgcgtttc gccgcgtttt ccgcactgcc gcccgtcaaa cgtaccgcca gataaccggc
    15721 gctgaaagag tcgccagctg cggtggtatc gatcactttt tctttcggca gtttcaccgc
    15781 cggaacatcc actaaccctt cgccagcaat ggacaccagg caagaatccg ccccgcgttt
    15841 caccaccact tctttcacgc ccgcgttatg ggtgcgcgca atgacgtctt ccaccggctg
    15901 ttgaccccac agcgcgtctt catcgtccag cgtcaggaag gcgatatccg tgcattccag
    15961 catttgttgg tacacctgct gtgtctcttc tttgctggcc cacaggcgcg gacgatagtt
    16021 attgtcgaaa atcacttttc cgccgttggc gcggcattcg cgcagcaggg aaagcagctt
    16081 ttcgcggctg gtcgggctta agatcgccag gctaatcccg ctcaggtaga gataatcgaa
    16141 attcgccagc tcttcgcaaa tcgccgcaga ctgctcactc tccagccaga atttggcggc
    16201 ggcttcgttc cgccagtagt agaacgtacg ctcgccggtg ctgtcggttt caatgtagta
    16261 aaggcccggc agacggtttt ccatccgttg ggtcagggaa gtatcaacgt tctcgccgtg
    16321 ccaggcgtcc agcatttgct ggctaaaact gtccgttccc agcgccgtta cgtaatgaac
    16381 ggttaatgcc gcaggatcga cctgacgggc gatatagacg gaagtgttca gggtatcgcc
    16441 gccgaaaccg cgcttaacgt ccgcgccttt ctcggaaagc tcaatcatgc attcgccaat
    16501 cacggcaatc tttttggaca tagtcgtgaa cctgatctgt aaaaaattag cgttagtttg
    16561 cgctgtggtg gtttgctggt caaccatatt aaaacagtgt tccattattt ttttgagcca
    16621 gaacgtattc ctgaaagatt ccgttgtgga gtgaggaaat ttaccccggc caggcggtaa
    16681 agttctgccc ttgcgcgccg ataatctttg tcgagtccgg gcagcatcac ttttaaacac
    16741 aggacatctt tgatgataag gcaggttatc cagcgaataa gcaaccctga agcaagcatc
    16801 gagagcttgc aggaacggcg tttttggttg cagtgtgagc gtgcttacac ctggcagccg
    16861 atctatcaaa catgcggacg gttaatggcc gtggagctat taacggtggt cacgcatccc
    16921 ttgaaccctt cgcaacgcct gccgccggat cgctatttta ctgaaatcac cgtcagccat
    16981 cggatggagg ttgtgaaaga gcagattgat ttgctggcgc aaaaagccga cttctttata
    17041 gagcacggcc tgctggcatc ggtcaatatt gatggcccta cgctcatcgc cctgcgtcag
    17101 caaccaaaaa tcctgcgcca gattgagcgt cttccctggc tgcgtttcga actggtggag
    17161 catatccgtc tgccgaaaga ttcaaccttt gcctcgatgt gtgaatttgg cccgctgtgg
    17221 ctggatgatt ttggtaccgg gatggcaaat ttctctgcac taagtgaagt gcgttatgac
    17281 tacatcaaaa tcgcgcgaga actgtttgtg atgctgcgtc agtcgccgga aggacgcaca
    17341 ctcttttctc agcttttaca tctaatgaat cgctattgtc gcggggtgat tgtcgagggc
    17401 gtagaaacgc cggaagagtg gcgtgatgtt cagaactcgc ccgcattcgc cgcacaaggc
    17461 tggtttcttt cacgcccggc accgatagaa acgctgaata cggcggttct ggcgctataa
    17521 gctgcctcat tttccgcctg gctggactat ctttaggact ggcacaggaa gaatatgagg
    17581 aagcgaacga tgagcaaggc aggcaaaata accgctgcga tttcagtggc tttcttgttg
    17641 ttgattgtcg tggcgatcat tttgattgca acttttgact ggaatcgact caaaccgacc
    17701 atcaaccaga aagtctctgc ggagttgaat cgtccgttcg ctatccgtgg cgatctgggc
    17761 gtggtgtggg agcggcaaaa acaagaaact ggctggcgca gctgggtgcc gtggccccat
    17821 gtacacgcgg aagacatcat tcttggcaat ccaccggata ttcccgaagt cacgatggtg
    17881 catttgccac gcgtagaggc aacgctggcc ccgctggcgc tgctgaccaa aacggtctgg
    17941 ctgccgtgga tcaagctcga aaagcccgac gcgcgcctga ttcgcctctc cgaaaagaac
    18001 aataactgga cgtttaatct cgccaacgat gataacaaag acgcgaatgc aaagccgtcg
    18061 gcatggtcgt ttcggctgga taatattctt ttcgatcaag ggcggatcgc cattgatgac
    18121 aaagtaagca aagcggatct ggagattttt gttgatccgt taggcaagcc gctgccgttc
    18181 agcgaagtta ctggatcgaa aggtaaagcg gataaagaaa aggtgggcga ttacgttttt
    18241 ggcctgaagg cgcagggacg ttataacggt gaaccgctca cgggtacggg aaaaataggc
    18301 ggtatgctgg cgctgcgtgg cgaagggacg ccgtttccgg tacaggctga tttccgctct
    18361 ggtaacaccc gtgttgcttt tgatggcgtc gtgaatgatc caatgaagat gggcggtgtc
    18421 gatttacggc ttaaattttc tggcgattca ctgggtgatc tctatgaact gacgggcgtt
    18481 ctgctgcccg ataccccgcc gtttgaaacg gatggtcggc tggtagcgaa aatcgacact
    18541 gaaaaatcgt cggtctttga ttatcgcggt tttaatgggc gaattggcga tagcgatatc
    18601 cacggttctc tggtctacac caccggaaag ccacgaccaa aactggaagg tgatgtcgag
    18661 tcgcggcaat tgcggctggc ggacctggga ccgttaattg gcgttgactc cgggaaaggg
    18721 gcagaaaagt cgaaacggtc tgaacagaag aagggcgaaa aaagcgttca gcctgcgggc
    18781 aaagtgctgc cttatgaccg cttcgaaacc gataaatggg acgttatgga tgccgatgtt
    18841 cgcttcaaag ggcggcgcat tgagcatggc agtagcctgc cgattagtga tctttctact
    18901 catatcatcc tcaaaaatgc tgacctgcgc ctgcaaccgc tgaaatttgg catggcgggc
    18961 ggcagcattg cggcgaatat tcatctggaa ggcgataaaa agccgatgca ggggcgggca
    19021 gatattcagg ctcgtcgact gaaactgaaa gaactgatgc ccgatgtgga actgatgcag
    19081 aagacgctgg gggaaatgaa cggtgacgcg gaactacgcg gtagcggtaa ctcggtggcg
    19141 gcacttttag gcaacagtaa cggcaacctg aaactgttga tgaatgacgg gctggtgagc
    19201 cgcaacctga tggagattgt tgggctgaat gtcggcaact acattgtcgg tgcgatattt
    19261 ggtgatgatg aggtgcgggt gaactgcgcg gcggcgaatc tggatattgc caacggcgtg
    19321 gcgcgcccgc agatctttgc tttcgatact gagaacgcgt tgattaacgt taccggcacg
    19381 gcaagttttg cttccgaaca gctggatttg actattgatc cggagagtaa aggaattcgg
    19441 attatcacac tgcgttcgcc gctgtatgtg cgtgggacgt ttaaaaatcc gcaggctggg
    19501 gtgaaagccg ggccgttgat tgcccgtggt gctgttgctg cggcactggc aacgctggta
    19561 acaccggcgg cggcgttact ggcactgatc tcaccttccg aaggggaggc taatcagtgt
    19621 cggacgatat tgtcgcagat gaagaagtga tgaggttgga ttgtgtttgc tggtgagttc
    19681 gggagcagcg tgttcccctc accctaaccc tctccccaaa ggggcgaggg gactgttttg
    19741 tgcgtgttga aacaacgagt accgcttttc cgttcacttt ctgggggagg gctgttatgt
    19801 tcgcgatata acatctcgct ccgcttttct ccctctccct ttcagggaga gggctggggt
    19861 gagggtggct gcccacacga tacttacaac gactgatgtc gcgtctcatg ggtcagcagc
    19921 agggcgatta acgtcaagcc agccatcgcc gccagatata accccaccgc acctaacccg
    19981 tagttagcct gcaaccaggc tgcgatatat ggtgcaacgg aagccccgag aatcgacgct
    20041 acgttgtaag agaacgatgc tccggtgtaa cgcacttctg tcgggaacag ctctggtaac
    20101 agcgcgccca tcggcccgaa ggtcagtccc atcagactta accccagcag caggaaggca
    20161 aaaaccagaa tcgggttgcc agaaccgagc agcgggttaa aggcgaacag cgcgaacagg
    20221 atgatcagcg tggtgatgat taccatgctt ttacggcgac caaaggcatc agccagtaat
    20281 ccggcgactg gcaccatcac gccaaaacca ataactgcca tcatcagcat ccacaacact
    20341 tcgttacgcg gcaggccaag cccaactggc gcggcggcgg tactgaaggt catagagtag
    20401 accgtcatga tgtaaaacag cgtataggtt gccagcataa tgaacgtacc cagtacggtt
    20461 acgcgaacat gtttggtcag cagcgtaccc agcgggatct tcacctgttt tttcgcttta
    20521 gcgactttct caaacaccgg cgactcatgc agcgacacgc gaacatacag gccgataatg
    20581 accagcaccg ccgagaagat aaatggcaca cgccagcccc agctcataaa ctgctcgtcg
    20641 gtcagtagcc aggaaagcag caagaaagtg ccgttggcaa agaagaagcc aatcggtgcg
    20701 cccagctgtg ggaaggagcc atacagtgca cgtttgcgcg gtggggcgtt ttcagtcgcc
    20761 agcagcgccg cgccgcccca ttcaccgcct aagcccagac cctgaccaaa tcgagccagc
    20821 gccagcagca gcggggcgaa aataccaatc gtggcatagc ccggcagcag accaatcacc
    20881 acggtcgaaa tccccatcgt tagcaacgag gcgaccagcg tcgctttacg cccaacgcga
    20941 tcgccaaaat gaccaaaaac ggcagagcca atggggcgcg cgacgaaggc gatggcgaag
    21001 gtggcgagcg actgtagcgt tgctgccgtt ggatcgccct gcgggaagaa gatatgcgga
    21061 aacacaataa cggccgcagt ggcgtaaatg taaaagtcga agaactcaat ggccgtgcca
    21121 atgagagagg cgacaaggac tttattacgc gagttgatcg gcgtgtattc ttgctcgtgg
    21181 tcgagtgttg tggctgttgc ttgcataatc ttttcttatt ttttggctaa cgaatagcca
    21241 tattacgcac agcaaaagtg atatttcaat gtgtaacaaa tcgcggaaat ggtcaaaaac
    21301 caggcaaaaa ggggataatg tttaggccag tgatttttct tctatgaaat gcttcacata
    21361 attttataaa tagtggataa aactggtttc aggttaaata aaagttacca actggattta
    21421 tatgctgggg ttatgagttg gaatgaaatt gtttgctccc aaatagatgg ctgaagaggc
    21481 tctggggaag agtttcaaga ggttatgtgc gcattatagc cggatgcgat gctggcgcat
    21541 cttatccggc ctacggggag ccaggccgaa taaaatcgta tgccggataa ggcgtttacg
    21601 ccgcatccgg cctattttaa ggctgcgttt tccctggcat tcgcgggtcg tctttatatt
    21661 cggcggtggc aatccacgcc gcgcaaaaca gcgtcaaacg ggcgaagaag tagaaaaatg
    21721 ccatcagccc cagcacggaa ccaaatgctg cgccagaggg ggatttcatc agcgatggca
    21781 aggtgtaggt catcacgatt ttaatcactt caaaaccaat agcagccaga aatgttccgc
    21841 gaatcagggc ttttttgcgc ggacggtggc gcggcaaccg ccagaagatc cagaagaaaa
    21901 gcagatagtt ggcgaagatg gaaatcgcca ggccaatcaa ccgccacgtc ggtttcagcc
    21961 actcaatgct gttcaggtgc agggcgctaa taatcatttg ctgcgccgaa ccggcaaccg
    22021 aggtgatcga cagcgtcaca atcagcgcaa ttaacaaacc aatcagtgaa ataaaatcac
    22081 gcagatattt tacccagaac ttctcctgat cttgcggcga gcgttcccag acatcgcgcg
    22141 actgggcacg aatcgcttca cgcaggttac ccatccagtt gatgccggaa taaagtgcca
    22201 ccgccaggcc gacaagccct acagtcgtac gctgctgaac ggcggtgttg atggtgtttt
    22261 tcaacgtggc ggctagcgtc ggatcgctga tgttttgcag aattttgtcg aagatatcct
    22321 gtagcaacat cggatgggag gccagcacaa agccccctgc ggcaaacgac accatcaaaa
    22381 tcgggatcat cgacaaaaat gagaaatagg tgatagccgc accaaactgg ttacccagcc
    22441 gatcgttaaa gcgttctgtc gcacgaatca ggtgcgcaat aaccggttgg cgttggactt
    22501 tttcggcagt ggtggtgacg gtttccagcg cctggctaac tttgccgcct ttctcgctat
    22561 tatccaactg tttaatcggc tcgtgctcca gatcctgggt gggacgtttg atctcgtttt
    22621 cctgcgtcat ccgctctttt gtccttctgt cgtggtcatt cttaaaaagt atagtcggtc
    22681 agtccaggtg ctctttcatt acgccagcca gccactgcat aaacaggttt acacgccggg
    22741 aaagctcccg acgctgcggg taaaccagcg aaagggagag cggctcggca cggtagcctg
    22801 gcaacacttc aataagccgc ccggcacgca gggcttcgcg cacggcaatg cgcgggatct
    22861 gaataatccc cagaccggca agaccggcgg cgagatagtt ttcgctactg tttaccgtca
    22921 acatgccgcc agacttaaac cactggacgc cattaacgct ggcaacctca aaacctaacg
    22981 gatgtacacc caggtgcggt gtataacgca ctattgcgtg tgaagtcaga tcatcggggc
    23041 tttgcggata accaaagcgc gtcagatagt gcggactggc acagttgacc atggtcagtt
    23101 tgccgagggg acgggcgata acgccatctt ccggtaacgc gccagtgcgt atcacgcaat
    23161 caaaaccatc atgaagaata tctaccggac ggtcatggct actcagttcc agctcaattc
    23221 ccggatgcag atagagaaat tccgacaggc gcggcagtaa caggcttttc gcgattcctg
    23281 gcgggatgtc gatgcgtaat ttaccgctga tactggtggc atcctgttga aacagaccgt
    23341 ccagttcgct gagattactc aacacatctt ttgctcgttg ataataggtc atgccttctg
    23401 gcgtgagttt gacccgtcgc gtggtgcgct ggagaagctg ggtgccaagt tgatgttcca
    23461 gtgcctgtat ctggcgcgaa acacttcctt ttggcaaagc aaagaaatcc gccgcgcggg
    23521 aaaaactttc cagctccgcg actttgatga acaactgcat tgcgtgaatt ttatccattt
    23581 ctttgaccga ttgttgttca cagcgaaaca gtaacgcgca tatcgcattg tttattgttt
    23641 tttctaacat ctaatgccgg gtttcacaat gttgtcatga cgaatgtggc gatttctcag
    23701 caggaaaagg gcggaatagc tttctgagaa aggtgaatcc ccctaaacag agcgtatttt
    23761 tcagcgattc cctttaaagt gaatagagga cgtgttggct atttgtgctt atttttgtct
    23821 cattgccgtt tgttattttt gcttgtggtc gcggagtttc atttttaatt gcatgcgtac
    23881 taaccaccaa tataaggaaa tcctcaaaat tgttttaaat agtgccctcg acatgtaatt
    23941 tcaggcgttt agctgtaact atttatatcg gtaactattt tctacgcgtc atctctgtag
    24001 aatggccacc gccaaatggc gaattcttat tttttctcgc gggtaatacc cttccactac
    24061 ttaagtcttt ttaacgtatt caggtggaga ttcagttatg caaatagtca tgtttgacag
    24121 gcagtcaata tttattcatg gaatgaaaat cagtttacag cagcgtattc caggagtgag
    24181 tattcagggg gccagtcagg cagacgagtt atggcaaaag ctggaaagtt accctgaagc
    24241 cttagttatg ctcgatggcg atcaggatgg tgagttttgc tactggttgc tgcaaaaaac
    24301 cgtggtgcag tttcctgagg ttaaggtgtt aatcacggcg acggattgca ataaacggtg
    24361 gttacaggaa gttatccatt ttaatgtgct ggccattgtg cctcgtgatt caaccgtcga
    24421 gacatttgcg ttggcggtaa atagtgcggc gatggggatg atgtttctgc ctggagactg
    24481 gcggacgacg ccggaaaagg acattaaaga tctcaaatcg ttaagcgccc gccagcggga
    24541 gattttaacc atgttagcgg cgggcgaatc aaataaggag atcggcagag cgctgaatat
    24601 cagtaccgga acggtaaaag cgcatctgga atccctgtat cgccgtctgg aagtgaaaaa
    24661 tcgcacccag gcggcaatga tgttaaatat ctcctcctga agaggataat tttacagcca
    24721 gcaggaagtt agcggctggc tgtaaaaata ttatggttcg ccgtacaaac caattaaacg
    24781 gcgtaccaca ccgttagtcc agccaaaccc atcctgcaac ggatactcgc caccgccgcc
    24841 ttcgcgggga acaccatcgg caatatggta tttctcgatc attttgtgct gttccagata
    24901 gaactgattc accgttttca gccagctgcg cgcgatttca tcacccagaa ggtcatcgcc
    24961 atacatttta aatccctgaa ttgccatcca ttgtaacggt gcccagccat tgggtttatc
    25021 ccactgttca ccggtttcgt actcgcttgc cagaatcccg ccaggtgtca gtaaccggct
    25081 gcgtacggcg tttgccagac gatcggcctg ttcatggttc gccatgccga catagagcgg
    25141 cacaatggcg gcagcggaaa acagcgccag ttgttcgcgt cgccagtcgt aatcgcggta
    25201 gatgccgttt tcatcatccc agaggtaacg gtttaccgca tcgcgacggg cactggcctt
    25261 ctggcggaac agcgcttctg tctctttctc gcctttcagc gccgagatgt tggcgatggc
    25321 gctctccagt ttaaacagga aggcattcag atcgatgggg atgaactggg tggtacgaat
    25381 gctcgccaga cgaccagtat cacgcagcca acgggaagag taatcccaac ctgaggccgc
    25441 tcccgcgcgt aaatcgcggt acacctcgtt gggcgggcga ccagaatgtt tcgcggtttc
    25501 aacgtcctca agccaggatt cgtcacgcgg cgtgtcgcga tcgtcccaat aacggttgag
    25561 cagcgatccg tccggcatcc gcacaacatg gcgataggcc tgattaggga tcaacgattc
    25621 tgcaccgtcc atccagaagg catattccat tttaaggtgg tcgagatagc ggcgcgcacc
    25681 gcgtacacca tcttcttcaa acaactccac catcagcgca aaaaccggtg gttgcgatcg
    25741 gctcaaataa taggtgcggt tgccgttggg gatgtgacca tagttttcga tcatccaggc
    25801 gaagttatcg gccatgcatt tcagcaaatc ttcccgacca ctttccgcca gccccagcat
    25861 ggtgaaatag gaatcccaat agtaggtttc gctaaaacgg ccgcccggga caatatatga
    25921 ctgcggtagc gccagcagag aagaccacgg aatgtgatcc tgtggttcgc gggttagcac
    25981 cggccacagc tggtcgatat gctctttcag ggaattttgc gggtccgata catactcgct
    26041 ggagtagacc tccggcagcc agaagtgatt ttcaacaaac ttgcgcaagt caaaatcacg
    26101 atgacggcgc actttacggt agcggattaa aatatccagc gggtccattt tgggtgcgca
    26161 gtcgggaaag gttttactgt cggggaaaat tttcgccgac tgaacatgct cgaacagttc
    26221 gagatagcga tcggcaggcg tcagcgcatc agaggcgggc agcccttcaa tcatctcggg
    26281 ttccggttct gcctcgatca tctcatccag ttttaattca tacgggtcca gttcatagca
    26341 gagatcgacc tcgatcatca gttcgtctgg attagggttt tgaattttct gattgagcat
    26401 aaggatccaa cctccgaaat tcgtcgctaa ttatcgggct tttctttaag ggtagtcatt
    26461 cgctccgcct ggtgggcgac aaagcgtgcg gtggatcacg tttattaact tgtcaaaaaa
    26521 tataacgcac taatatcaat ggaatttata aaaatacgga tttcgtgttg tttttcattt
    26581 tcagaacatt caatcctgct ctcgccgcaa tcatgatatg ccattcctga tctgggtaat
    26641 aggcacaggc tatcttat
//
LOCUS       DAZKHF020000036        26650 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2201S31241
            SAMN43267800-rid25106413.denovo.036, whole genome shotgun sequence.
ACCESSION   DAZKHF020000036 DAZKHF020000000
VERSION     DAZKHF020000036.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267800
            Sequence Read Archive: SRR30307263
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 26650)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 26650)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 26650)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350581.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350581.2
            Genome Coverage       :: 151x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:21:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,652
            CDSs (total)                      :: 5,557
            Genes (coding)                    :: 5,320
            CDSs (with protein)               :: 5,320
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 237
            CDSs (without protein)            :: 237
            Pseudo Genes (ambiguous residues) :: 0 of 237
            Pseudo Genes (frameshifted)       :: 102 of 237
            Pseudo Genes (incomplete)         :: 130 of 237
            Pseudo Genes (internal stop)      :: 52 of 237
            Pseudo Genes (multiple problems)  :: 41 of 237
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26650
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267800-rid25106413.denovo.036"
                     /serotype="O 26:H11"
                     /isolate="2201S31241"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-01"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            51..1649
                     /gene="rtcR"
                     /locus_tag="ACHOSC_RS06175"
                     /old_locus_tag="pgaptmp_001235"
     CDS             51..1649
                     /gene="rtcR"
                     /locus_tag="ACHOSC_RS06175"
                     /old_locus_tag="pgaptmp_001235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator RtcR"
                     /protein_id="HGU4547211.1"
                     /translation="MRKTVAFGFVGTVLDYAGRGSQRWSKWRPTLCLCQQESLVIDRL
                     ELLHDTRSRSLFETLKRDIASVSPETQVVGVEIELHNPWDFEEVYACLHDFARGYEFQ
                     PEKEDYLIHITTGTHVAQICWFLLAEARYLPARLIQSSPPRKKEQPRGAGEVTIIDLD
                     LSRYNAIASRFAEERQQTLDFLKSGIATRNPHFNRMIEQIEKVAIKSRAPILLNGPTG
                     AGKSFLARRIFELKQARHQFSGAFVEVNCATLRGDTAMSTLFGHVKGAFTGARESREG
                     LLRSANGGMLFLDEIGELGADEQAMLLKAIEEKTFYPFGSDRQVSSDFQLIAGTVRDL
                     RQLVAEGKFREDLYTRINLWTFTLPGLRQRQEDIEPNLDYEVERHASLTGDSVRFNTE
                     ARRAWLAFATSPQATWRGNFRELSASVTRMATFATSGRITLDVVEDEINRLRYNWQES
                     RPSALTALLGAEAENIDLFDRMQLEHVIAICRQAKSLSAAGRQLFDVSRQGKASVNDA
                     DRLRKYLARFGLTWEAVQDQHSSS"
     gene            complement(1631..2389)
                     /locus_tag="ACHOSC_RS06180"
                     /old_locus_tag="pgaptmp_001236"
     CDS             complement(1631..2389)
                     /locus_tag="ACHOSC_RS06180"
                     /old_locus_tag="pgaptmp_001236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000815090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR family transcriptional regulator"
                     /protein_id="HGU4547212.1"
                     /translation="MKQTQRHNGIIELVKQQGYVSTEELVEHFSVSPQTIRRDLNELA
                     EQNLILRHHGGAALPSSSVNTPWHDRKATQTEEKERIARKVAEQIPNGSTLFIDIGTT
                     PEAVAHALLNHSNLRIVTNNLNVANTLMVKEDFRIILAGGELRSRDGGIIGEATLDFI
                     SQFRLDFGILGISGIDSDGSLLEFDYHEVRTKRAIIENSRHVMLVVDHSKFGRNAMVN
                     MGSISMVDAVYTDAPPPVSVMQVLTDHHIQLELC"
     gene            complement(2406..3236)
                     /gene="glpG"
                     /locus_tag="ACHOSC_RS06185"
                     /old_locus_tag="pgaptmp_001237"
     CDS             complement(2406..3236)
                     /gene="glpG"
                     /locus_tag="ACHOSC_RS06185"
                     /old_locus_tag="pgaptmp_001237"
                     /EC_number="3.4.21.105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease
                     GlpG"
                     /protein_id="HGU4547213.1"
                     /translation="MLMITSFANPRVAQAFVDYMATQGVILTIQQHNQSDVWLADESQ
                     AERVRAELARFLENPADPRYLAASWQAGHTGSGLHYRRYPFFAALRERAGPVTWVVMI
                     ACVVVFIAMQILGDQEVMLWLAWPFDPTLKFEFWRYFTHALMHFSLMHILFNLLWWWY
                     LGGAVEKRLGSGKLIVITLISALLSGYVQQKFSGPWFGGLSGVVYALMGYVWLRGERD
                     PQSGIYLQRGLIIFALIWIVAGWFDLFGMSMANGAHIAGLAVGLAMAFVDSLNARKRK
                     "
     gene            complement(3281..3607)
                     /gene="glpE"
                     /locus_tag="ACHOSC_RS06190"
                     /old_locus_tag="pgaptmp_001238"
     CDS             complement(3281..3607)
                     /gene="glpE"
                     /locus_tag="ACHOSC_RS06190"
                     /old_locus_tag="pgaptmp_001238"
                     /EC_number="2.8.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004792 - thiosulfate sulfurtransferase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiosulfate sulfurtransferase GlpE"
                     /protein_id="HGU4547214.1"
                     /translation="MDLFECINVADAHQKLQEKEAVLVDIRDPQSFAMGHAVQAFHLT
                     NDTLGAFMRDNDFDTPVMVMCYHGNSSKGAAQYLLQQGYDVVYSIDGGFEAWQRQFPA
                     EVAYGA"
     gene            3797..5302
                     /gene="glpD"
                     /locus_tag="ACHOSC_RS06195"
                     /old_locus_tag="pgaptmp_001239"
     CDS             3797..5302
                     /gene="glpD"
                     /locus_tag="ACHOSC_RS06195"
                     /old_locus_tag="pgaptmp_001239"
                     /EC_number="1.1.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009331 - glycerol-3-phosphate
                     dehydrogenase (FAD) complex [Evidence IEA];
                     GO_function: GO:0004368 - glycerol-3-phosphate
                     dehydrogenase (quinone) activity [Evidence IEA];
                     GO_process: GO:0006072 - glycerol-3-phosphate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate dehydrogenase"
                     /protein_id="HGU4547215.1"
                     /translation="METKDLIVIGGGINGAGIAADAAGRGLSVLMLEAQDLACATSSA
                     SSKLIHGGLRYLEHYEFRLVSEALAEREVLLKMAPHIAFPMRFRLPHRPHLRPAWMIR
                     IGLFMYDHLGKRTSLPGSTGLRFGANSVLKPEIKRGFEYSDCWVDDARLVLANAQMVV
                     RKGGEVLTRTRATSARRENGLWIVEAEDIDTGKKYSWQARGLVNATGPWVKQFFDEGM
                     HLPSPYGIRLIKGSHIVVPRVHTQKQAYILQNEDKRIVFVIPWMDEFSIIGTTDVEYK
                     GDPKAVKIEESEINYLLKVYNAHFKKQLSRDDIVWTYSGVRPLCDDESDSPQAITRDY
                     TLDIHDENGKAPLLSVFGGKLTTYRKLAEHALEKLTPYYQGIGPAWTKESVLPGGAIE
                     GDRDDYAARLRRRYPFLTESLARHYARTYGSNSELLLGNAGAISDLGEDFGHEFYEAE
                     LKYLVDHEWVRRTDDALWRRTKQGMWLNADQQSRVSQWLVEYTQQKLSLAS"
     gene            complement(5354..5959)
                     /locus_tag="ACHOSC_RS06200"
                     /old_locus_tag="pgaptmp_001240"
     CDS             complement(5354..5959)
                     /locus_tag="ACHOSC_RS06200"
                     /old_locus_tag="pgaptmp_001240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000589351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4547216.1"
                     /translation="MIKFRLYIPPVILGFVIVPLLVWPTVIVLAVLIFTLTFLAEIIF
                     SFPLLVVRISLQELQLELMVEYALFFSVMAGIGWQFSRRTPPELKNRLHCWLVFSPVY
                     FWLILSNFILYISPEKSALLENIRNFFLTFVWPPLNFSPFWPQPWTDFVGPISAQLGF
                     AWGYYCHWCRKNRNQRKKWGDWVTCLSLAILAQGPLFNYLQ"
     gene            complement(5962..6729)
                     /locus_tag="ACHOSC_RS06205"
                     /old_locus_tag="pgaptmp_001241"
     CDS             complement(5962..6729)
                     /locus_tag="ACHOSC_RS06205"
                     /old_locus_tag="pgaptmp_001241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001182130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4547217.1"
                     /translation="MQPCPAMQALIEQIYHTFRRYRVPQKFVVCCEYCLSQQEQKALR
                     STSLRAIPYSLINAWNSSPGPDPQNSDEVRYFLPRLLEFVAQGHFDNIHVVFSLRRIN
                     LANKENWRADERAILQRFACQYMTDWVSGDEAVELQYMLEMFFRADIALAPLLDAINS
                     VPGFWSTVSLACLLNRYCEDYIRDNQDDIDNAITTQINAWARNNHPLLKERARQAIEN
                     PLKQPEPMTEYQVWEDDWIIDECLCAMYDASSESPGK"
     gene            complement(6732..7442)
                     /locus_tag="ACHOSC_RS06210"
                     /old_locus_tag="pgaptmp_001242"
     CDS             complement(6732..7442)
                     /locus_tag="ACHOSC_RS06210"
                     /old_locus_tag="pgaptmp_001242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000424214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4547218.1"
                     /translation="MELVNTLFASLAGTDPFTGVDITIANYKSAYWDEGIVQQLINQA
                     LDEGEKFVGADGLEGLLRYDVTLNIGLTSSKVWPGFSLDTATISRLCACGADFGFDPY
                     ISDVPDVQCDLNTTNDVTVQFTAMLNPDERVIIAKRPLKKCDSWIEDVYIFQVFKDAW
                     KFHNNNSLRGFRDKQAELKLYTRHYSVENCAEESCRDCNSCIRPSFSLSRSALIRLNA
                     ANARFVYQPFTRDQRARG"
     gene            complement(7458..8966)
                     /locus_tag="ACHOSC_RS06215"
                     /old_locus_tag="pgaptmp_001243"
     CDS             complement(7458..8966)
                     /locus_tag="ACHOSC_RS06215"
                     /old_locus_tag="pgaptmp_001243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001443257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PstS family phosphate ABC transporter
                     substrate-binding protein"
                     /protein_id="HGU4547219.1"
                     /translation="MMQNRKWILSSLVMTFFGIPILTQFLAAVVAMLGVGLAGIIEVC
                     NILITPTSYLLLNIFMLALGALMLFFSGRVWAGDSAPEKREIAVWRQCLFLVPGLLIL
                     VGWIIALHLADYQFHQMGSGWLADLMLPWLGVLLVSVVGGEYWWIVIIPVGAHISFSL
                     GYGRPTRHPLTGTSGLRCRNSLLFILLMLGFVAGYQGYLYKQLNPGVGVRENIDTWAW
                     RPDKLNNQLTPLRGKPQIQFTQNWPRLDGATAAYPIYASAFYALSVIPEDFHTREYLE
                     SSRTPDAYNRIVKGDADIIFVAQPSGGQKKRAEESGVTLLYTPFAREAFVFIVNADNP
                     VNSLTEQQVRDIFSGAITNWRTVGGNDQEIQTWQRPEDSGSQTVMQSQVMKNVRMISP
                     QETEVASMMEGMIKVVAEYRNTNNAIGYTFRYYATQMNADKNIKLLAINGIAPTAENI
                     RNGKYPYIVDAFMITRENTTSETQKLLEWFLTPQGQSLVEDVGYVPMYKTLP"
     gene            complement(9092..11539)
                     /gene="glgP"
                     /locus_tag="ACHOSC_RS06220"
                     /old_locus_tag="pgaptmp_001244"
     CDS             complement(9092..11539)
                     /gene="glgP"
                     /locus_tag="ACHOSC_RS06220"
                     /old_locus_tag="pgaptmp_001244"
                     /EC_number="2.4.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008184 - glycogen phosphorylase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen phosphorylase"
                     /protein_id="HGU4547220.1"
                     /translation="MNAPFTYSSPTLSVEALKHSIAYKLMFTIGKDPVVANKHEWLNA
                     TLFAVRDRLVERWLRSNRAQLSQETRQVYYLSMEFLIGRTLSNAMLSLGIYEDVQGAL
                     EAMGLNLEELIDEENDPGLGNGGLGRLAACFLDSLATLGLPGRGYGIRYDYGMFKQNI
                     VNGSQKESPDYWLEYGNPWEFKRHNTRYKVRFGGRIQQEGKKTRWIETEEILGVAYDQ
                     IIPGYDTDATNTLRLWSAQASSEINLGKFNQGDYFAAVEDKNHSENVSRVLYPDDSTY
                     SGRELRLRQEYFLVSSTIQDILSRHYQLHKTYDNLADKIAIHLNDTHPVLSIPEMMRL
                     LIDEHQFSWDDAFEVCCQVFSYTNHTLMSEALETWPVDMLGKILPRHLQIIFEINDYF
                     LKTLQEQYPNDTDLLGRASIIDESNGRRVRMAWLAVVVSHKVNGVSELHSNLMVQSLF
                     ADFAKIFPGRFTNVTNGVTPRRWLAVANPSLSAVLDEHLGRNWRTDLSLLNELQQHCD
                     FPMVNHAVHQAKLENKKRLAEYIAQQLNVVVNPKALFDVQIKRIHEYKRQLMNVLHVI
                     TRYNRIKADPDAKWVPRVNIFGGKAASAYYMAKHIIHLINDVAKVINNDPQIGDKLKV
                     VFIPNYSVSLAQLIIPAADLSEQISLAGTEASGTSNMKFALNGALTIGTLDGANVEML
                     DHVGADNIFIFGNTAEEVEELRRQGYKPREYYEKDEELHQVLTQIGSGVFSPEDPGRY
                     RDLVDSLINFGDHYQVLADYRSYVDCQDKVDELYELQEEWTAKAMLNIANMGYFSSDR
                     TIKEYADHIWHIDPVRL"
     gene            complement(11558..12991)
                     /gene="glgA"
                     /locus_tag="ACHOSC_RS06225"
                     /old_locus_tag="pgaptmp_001245"
     CDS             complement(11558..12991)
                     /gene="glgA"
                     /locus_tag="ACHOSC_RS06225"
                     /old_locus_tag="pgaptmp_001245"
                     /EC_number="2.4.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004373 - glycogen (starch) synthase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen synthase GlgA"
                     /protein_id="HGU4547221.1"
                     /translation="MQVLHVCSEMFPLLKTGGLADVIGALPAAQIADGVDARVLLPAF
                     PDIRRGVTDAQVVSRRDTFAGHITLLFGHYNGVGIYLIDAPHLYDRPGSPYHDTNLFA
                     YTDNVLRFALLGWVGAEMASGLDPFWRPDVVHAHDWHAGLAPAYLAARGRPAKSVFTV
                     HNLAYQGMFYAHHMNDIQLPWSFFNIHGLEFNGQISFLKAGLYYADHITAVSPTYARE
                     ITEPQFAYGMEGLLQQRHREGRLSGVLNGVDEKIWSPETDLLLASRYTRDTLEDKAEN
                     KRQLQIAMGLKVDDKVPLFAVVSRLTSQKGLDLVLEALPGLLEQGGQLALLGAGDPVL
                     QEGFLAAAAEYPGQVGVQIGYHEAFSHRIMGGADVILVPSRFEPCGLTQLYGLKYGTL
                     PLVRRTGGLADTVSDCSLENLADGVASGFVFEDSNAWSLLRAIRRAFVLWSRPSLWRF
                     VQRQAMAMDFSWQVAAKSYRELYYRLK"
     gene            complement(12991..14286)
                     /gene="glgC"
                     /locus_tag="ACHOSC_RS06230"
                     /old_locus_tag="pgaptmp_001246"
     CDS             complement(12991..14286)
                     /gene="glgC"
                     /locus_tag="ACHOSC_RS06230"
                     /old_locus_tag="pgaptmp_001246"
                     /EC_number="2.7.7.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008878 - glucose-1-phosphate
                     adenylyltransferase activity [Evidence IEA];
                     GO_process: GO:0005978 - glycogen biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate adenylyltransferase"
                     /protein_id="HGU4547222.1"
                     /translation="MVSLEKNDHLMLARQLPLKSVALILAGGRGTRLKDLTNKRAKPA
                     VHFGGKFRIIDFALSNCINSGIRRMGVITQYQSHTLVQHIQRGWSFFNEEMNEFVDLL
                     PAQQRMKGENWYRGTADAVTQNLDIIRRYKAEYVVILAGDHIYKQDYSRMLIDHVEKG
                     ARCTVACMPVPIEEASAFGVMAVDENDKIIEFVEKPANPPSMPNDPSKSLASMGIYVF
                     DADYLYELLEEDDRDENSSHDFGKDLIPKITEAGLAYAHPFPLSCVQSDPDAEPYWRD
                     VGTLEAYWKANLDLASVVPELDMYDRNWPIRTYNESLPPAKFVQDRSGSHGMTLNSLV
                     SGGCVISGSVVVQSVLFSRVRVNSFCNIDSAVLLPEVWVGRSCRLRRCVIDRACVIPE
                     GMVIGENAEEDARRFYRSEEGIVLVTREMLRKLGHKQER"
     gene            complement(14304..16277)
                     /gene="glgX"
                     /locus_tag="ACHOSC_RS06235"
                     /old_locus_tag="pgaptmp_001247"
     CDS             complement(14304..16277)
                     /gene="glgX"
                     /locus_tag="ACHOSC_RS06235"
                     /old_locus_tag="pgaptmp_001247"
                     /EC_number="3.2.1.196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004133 - glycogen debranching enzyme
                     activity [Evidence IEA];
                     GO_process: GO:0005980 - glycogen catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen debranching protein GlgX"
                     /protein_id="HGU4547223.1"
                     /translation="MTQLAIGKPAPLGAHYDGQGVNFTLFSAHAERVELCVFDANGQE
                     HRYDLPGHSGDIWHGYLPDARPGLRYGYRVHGPWQPAEGHRFNPAKLLIDPCARQIDG
                     EFKDNPLLHAGHNEPDYRDNAAIAPKCVVVVDHYDWEDDAPPRTPWGSTIIYEAHVKG
                     LTYLHPEIPVEIRGTYKALGHPVMINYLKQLGITALELLPVAQFASEPRLQRMGLSNY
                     WGYNPVAMFALHPAYACSPETALDEFRDAIKALHKAGIEVILDIVLNHSAELDLDGPL
                     FSLRGIDNRSYYWIREDGDYHNWTGCGNTLNLSHPAVVDYASACLRYWVETCHVDGFR
                     FDLAAVMGRTPEFRQDAPLFTAIQNCPVLSQVKLIAEPWDIAPGGYQVGNFPPLFAEW
                     NDHFRDAARRFWLHYDLPLGAFAGRFAASSDVFKRNGRLPSAAINLVTAHDGFTLRDC
                     VCFNHKHNEANGEENRDGTNNNYSNNHGKEGLGGSLDLVERRRDSIHALLTTLLLSQG
                     TPMLLAGDEHGHSQHGNNNAYCQDNQLTWLDWSQASSGLTAFTAALIHLRKRIPALVE
                     NRWWEEGDGNVRWLNRYAQPLSTDEWQNGPKQLQILLSDRFLIAINATLEVTEIVLPA
                     GEWHAIPPFAGEDNPVITAVWQGPAHGLCVFQR"
     gene            complement(16274..18460)
                     /gene="glgB"
                     /locus_tag="ACHOSC_RS06240"
                     /old_locus_tag="pgaptmp_001248"
     CDS             complement(16274..18460)
                     /gene="glgB"
                     /locus_tag="ACHOSC_RS06240"
                     /old_locus_tag="pgaptmp_001248"
                     /EC_number="2.4.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003844 - 1,4-alpha-glucan branching
                     enzyme activity [Evidence IEA];
                     GO_process: GO:0005978 - glycogen biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-alpha-glucan branching enzyme"
                     /protein_id="HGU4547224.1"
                     /translation="MSDRIDRDVINALIAGHFADPFSVLGMHKTTAGLEVRALLPDAT
                     DVWVIEPKTGRKLAKLECLDSRGFFSGVIPRRKNFFRYQLAVVWHGQQNLIDDPYRFG
                     PLIQEMDAWLLSEGTHLRPYETLGAHADTMDGVTGTRFSVWAPNARRVSVVGQFNYWD
                     GRRHPMRLRKESGIWELFIPGAHNGQLYKYEMIDANGNLRLKSDPYAFEAQMRPETAS
                     LICGLPEKVVQTEERKKANQFDAPISIYEVHLGSWRRHTDNNFWLSYRELADQLVPYA
                     KWMGFTHLELLPINEHPFDGSWGYQPTGLYAPTRRFGTRDDFRYFIDAAHAAGLNVIL
                     DWVPGHFPTDDFALAEFDGTNLYEHSDPREGYHQDWNTLIYNYGRREVSNFLVGNALY
                     WIERFGIDALRVDAVASMIYRDYSRKEGEWIPNEFGGRENLEAIEFLRNTNRILGEQV
                     SGAVTMAEESTDFPGVSRPQDMGGLGFWYKWNLGWMHDTLDYMKLDPVYRQYHHDKLT
                     FGILYNYTENFVLPLSHDEVVHGKKSILDRMPGDAWQKFANLRAYYGWMWAFPGKKLL
                     FMGNEFAQGREWNHDASLDWHLLEGGDNWHHGVQRLVRDLNLTYRHHKAMHELDFDPY
                     GFEWLVVDDKERSVLIFVRRDKEGNEIIVASNFTPVPRHDYRFGINQPGKWREILNTD
                     SMHYHGSNAGNGGTVHSDEIASHGRQHSLSLTLPPLATIWLVREAE"
     gene            complement(18733..19836)
                     /gene="asd"
                     /locus_tag="ACHOSC_RS06245"
                     /old_locus_tag="pgaptmp_001249"
     CDS             complement(18733..19836)
                     /gene="asd"
                     /locus_tag="ACHOSC_RS06245"
                     /old_locus_tag="pgaptmp_001249"
                     /EC_number="1.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004073 - aspartate-semialdehyde
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0009086 - methionine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009088 - threonine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA];
                     GO_process: GO:0009097 - isoleucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate-semialdehyde dehydrogenase"
                     /protein_id="HGU4547225.1"
                     /translation="MKNVGFIGWRGMVGSVLMQRMVEERDFDAIRPVFFSTSQLGQAA
                     PSFGGTTGTLQDAFDLEALKALDIIVTCQGGDYTNEIYPKLRESGWQGYWIDAASSLR
                     MKDDAIIILDPVNQDVITDGLNNGIRTFVGGNCTVSLMLMSLGGLFANDLVDWVSVAT
                     YQAASGGGARHMRELLTQMGHLYGHVADELATPSSAILDIERKVTTLTRSGELPVDNF
                     GVPLAGSLIPWIDKQLDNGQSREEWKGQAETNKILNTSSVIPVDGLCVRVGALRCHSQ
                     AFTIKLKKDVSIPTVEELLAAHNPWAKVVPNDREITMRELTPAAVTGTLTTPVGRLRK
                     LNMGPEFLSAFTVGDQLLWGAAEPLRRMLRQLA"
     gene            20028..20621
                     /gene="yhgN"
                     /locus_tag="ACHOSC_RS06250"
                     /old_locus_tag="pgaptmp_001250"
     CDS             20028..20621
                     /gene="yhgN"
                     /locus_tag="ACHOSC_RS06250"
                     /old_locus_tag="pgaptmp_001250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAAT family transporter YhgN"
                     /protein_id="HGU4547226.1"
                     /translation="MNEIISAAVLLILIMDPLGNLPIFMSVLKHTEPKRRRAIMVREL
                     LIALLVMLVFLFAGEKILAFLSLRAETVSISGGIILFLIAIKMIFPSASGNSSGLPAG
                     EEPFIVPLAIPLVAGPTILATLMLLSHQYPNQMGHLVIALLLAWGGTFVILLQSSLFL
                     RLLGEKGVNALERLMGLILVMMATQMFLDGIRMWMKG"
     gene            complement(20678..22018)
                     /gene="gntU"
                     /locus_tag="ACHOSC_RS06255"
                     /old_locus_tag="pgaptmp_001251"
     CDS             complement(20678..22018)
                     /gene="gntU"
                     /locus_tag="ACHOSC_RS06255"
                     /old_locus_tag="pgaptmp_001251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015128 - gluconate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0035429 - gluconate transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gluconate transporter"
                     /protein_id="HGU4547227.1"
                     /translation="MTTLTLVLTAVGSVLLLLFLVMKARMHAFLALMVVSMGAGLFSG
                     MPLDKIAATMEKGMGGTLGFLAVVVALGAMFGKILHETGAVDQIAVKMLKSFGHSRAH
                     YAIGLAGLVCALPLFFEVAIVLLISVAFSMARHTGTNLVKLVIPLFAGVAAAAAFLVP
                     GPAPMLLASQMNADFGWMILIGLCAAIPGMIIAGPLWGNFISRYVELHIPDDISEPHL
                     GEGKMPSFGFSLSLILLPLVLVGLKTIAARFVPEGSTAYEWFEFIGHPFTAILVACLV
                     AIYGLAMRQGMPKDKVMEICGHALQPAGIILLVIGAGGVFKQVLVDSGVGPALGEALT
                     GMGLPIAITCFVLAAAVRIIQGSATVACLTAVGLVMPVIEQLNYSGAQMAALSICIAG
                     GSIVVSHVNDAGFWLFGKFTGATEAETLKTWTMMETILGTVGAIVGMIAFQLLS"
     gene            complement(22022..22549)
                     /gene="gntK"
                     /locus_tag="ACHOSC_RS06260"
                     /old_locus_tag="pgaptmp_001252"
     CDS             complement(22022..22549)
                     /gene="gntK"
                     /locus_tag="ACHOSC_RS06260"
                     /old_locus_tag="pgaptmp_001252"
                     /EC_number="2.7.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gluconokinase"
                     /protein_id="HGU4547228.1"
                     /translation="MSTTNHDHHIYVLMGVSGSGKSAVASEVAHQLHAAFLDGDFLHP
                     RRNIEKMASGEPLNDDDRKPWLQALNDAAFAMQRTNKVSLIVCSALKKHYRDLLREGN
                     PNLSFIYLKGDFDVIESRLKARKGHFFKTQMLVTQFETLQEPGADETDVLVVDIDQPL
                     EGVVASTIEVIKKGK"
     gene            complement(22688..23683)
                     /gene="gntR"
                     /locus_tag="ACHOSC_RS06265"
                     /old_locus_tag="pgaptmp_001253"
     CDS             complement(22688..23683)
                     /gene="gntR"
                     /locus_tag="ACHOSC_RS06265"
                     /old_locus_tag="pgaptmp_001253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001693277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gluconate operon transcriptional repressor GntR"
                     /protein_id="HGU4547229.1"
                     /translation="MKKKRPVLQDVADRVGVTKMTVSRFLRNPEQVSVALRGKIAAAL
                     DELGYIPNRAPDILSNATSRAIGVLLPSLTNQVFAEVLRGIESVTDAHGYQTMLAHYG
                     YKPEMEQERLESMLSWNIDGLILTERTHTPRTLKMIEVAGIPVVELMDSQSPCLDIAV
                     GFDNFEAARQMTTAIIARGHCHIAYLGARLDERTIIKQKGYEQAMLDAGLVPYSVMVE
                     QSSSYSSGIELIRQARREYPQLDGVFCTNDDLAVGAAFECQRLGLKVPDDMAIAGFHG
                     HDIGQVMEPRLASVLTPRERMGSIGAERLLARIRGESVTPKMLDLGFTLSPGGSI"
     gene            complement(23907..24602)
                     /gene="yhhW"
                     /locus_tag="ACHOSC_RS06270"
                     /old_locus_tag="pgaptmp_001254"
     CDS             complement(23907..24602)
                     /gene="yhhW"
                     /locus_tag="ACHOSC_RS06270"
                     /old_locus_tag="pgaptmp_001254"
                     /EC_number="1.13.11.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004213399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quercetin 2,3-dioxygenase"
                     /protein_id="HGU4547230.1"
                     /translation="MIYLRKANERGHANHGWLDSWHTFSFANYYDPNFMGFSALRVIN
                     DDVIEAGQGFGTHPHKDMEILTYVLEGTVEHQDSMGNKEQVPAGEFQIMSAGTGIRHS
                     EYNPSSTERLHLYQIWIMPEENGITPRYEQRRFDAVQGKQLVLSPDARDGSLKVHQDM
                     ELYRWALLKDEQSVHQIAAERRVWIQVVKGNVTINGVKASTSDGLAIWDEQAISIHAD
                     SDSEVLLFDLPPV"
     gene            complement(24725..25762)
                     /gene="yhhX"
                     /locus_tag="ACHOSC_RS06275"
                     /old_locus_tag="pgaptmp_001255"
     CDS             complement(24725..25762)
                     /gene="yhhX"
                     /locus_tag="ACHOSC_RS06275"
                     /old_locus_tag="pgaptmp_001255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709215.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oxidoreductase"
                     /protein_id="HGU4547231.1"
                     /translation="MVINCAFIGFGKSTTRYHLPYVLNRKDSWHVAHIFRRHAKPEEQ
                     APIYSHIHFTSDLDEVLNDPDVKLVVVCTHADSHFEYAKRALEAGKNVLVEKPFTPTL
                     AQAKELFALAKSKGLTVTPYQNRRFDSCFLTAKKAIESGKLGEIVEVESHFDYYRPVA
                     ETKPGLPQDGAFYGLGVHTMDQIISLFGRPDHVAYDIRSLRNKANPDDTFEAQLFYGD
                     LKAIVKTSHLVKIDYPKFIVHGKKGSFIKYGIDQQETSLKANIMPGEPGFAADNSVGV
                     LEYVNDEGVTVREEMKPEMGDYGRVYDALYQTITHGAPNYVKESEVLTNLEILERGFE
                     QASPSTVTLAN"
     gene            26095..26583
                     /gene="yhhY"
                     /locus_tag="ACHOSC_RS06280"
                     /old_locus_tag="pgaptmp_001256"
     CDS             26095..26583
                     /gene="yhhY"
                     /locus_tag="ACHOSC_RS06280"
                     /old_locus_tag="pgaptmp_001256"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
                     /protein_id="HGU4547232.1"
                     /translation="MSEIVIRHAETRDYEAIRQIHAQPEVYCNTLQVPHPSDHMWQER
                     LADRPGIKQLVACIDGDVVGHLTIDVQQRPRRSHVADFGICVDSRWKNRGVASALMRE
                     MIEMCDNWLRVDRIELTVFVDNAPAIKVYKKYGFEIEGTGKKYALRNGEYVDAYYMAR
                     VK"
BASE COUNT         6646 a         7140 c         6651 g         6213 t
ORIGIN      
        1 aaaaccaatt caccgataat gaaatatcgt cttttataag gatatctaag atgcgtaaaa
       61 cagtggcttt tggctttgtc ggtaccgtgc tggattatgc cggacgcggc agtcagcgct
      121 ggtcgaaatg gcgtccgacg ctttgtttat gccagcaaga atcgttagtc atcgatcggc
      181 tggaattgtt gcacgacacc cgctcgcgct cgctattcga aacgcttaaa cgcgatatcg
      241 ccagcgtttc gccagaaaca caagtcgtgg gcgttgagat tgaactgcat aacccgtggg
      301 atttcgaaga ggtttacgcc tgcctgcatg atttcgcccg tggttacgag tttcagccag
      361 aaaaagaaga ctatttaatt cacatcacca ccggcaccca cgtcgcgcag atttgctggt
      421 ttctgctggc ggaagcgcgt tacctgcccg cccggctgat acaatcttca cctccgcgca
      481 aaaaagaaca gccgcgcggc gcaggtgaag tgacgattat cgatctcgat ttaagccgtt
      541 ataacgccat cgccagccgc tttgccgagg aacgccagca aacgcttgat tttcttaagt
      601 ccggcattgc cacgcgtaac ccccacttca accgcatgat tgagcagatc gaaaaagtgg
      661 cgatcaaatc ccgcgcgccg attctgctta acggtccaac cggcgcgggc aagtcatttc
      721 tggcgcgacg catcttcgag ttaaaacagg cgcggcatca gtttagcggc gcgtttgtgg
      781 aagtgaactg cgccaccctg cgcggcgata ccgccatgtc gacgctgttt ggtcatgtaa
      841 aaggcgcgtt taccggggcg cgggaatctc gtgaaggttt attacgcagc gccaacggcg
      901 gaatgttgtt tcttgatgag attggcgaac tgggcgcaga cgaacaggca atgctgctaa
      961 aagccattga agagaaaacc ttttacccgt ttggcagcga tcgccaggtg agcagtgatt
     1021 ttcagcttat cgccggaacg gtgcgcgatt tgcgccagct ggttgccgaa ggcaaatttc
     1081 gcgaagatct gtacacgcgg atcaatctct ggaccttcac cctgccgggt ctacgccagc
     1141 gccaggaaga tattgaaccg aacctggatt atgaagtgga gcgccacgcc tcactcactg
     1201 gcgacagcgt gcgttttaac accgaagcgc ggcgcgcctg gttggctttt gcgacctctc
     1261 cccaggcaac atggcgcggt aactttcgcg aactttctgc cagcgtcacg cggatggcca
     1321 cctttgccac tagcggacgc atcactctgg acgtggttga agatgagata aaccgtctgc
     1381 gctataactg gcaggagagc cgcccctccg cgcttacggc gttgctgggc gcagaggcag
     1441 aaaacatcga tctcttcgac cgtatgcaac tggaacacgt tatcgctatc tgccgccagg
     1501 caaagtcgct ttccgcagcc ggacgccagc tttttgacgt ttcgcgccag ggcaaagcca
     1561 gcgtcaatga cgcggatcgg ctacgcaaat acctggcgcg ttttggtctg acgtgggaag
     1621 ccgtgcagga tcagcacagc tccagttgaa tatggtggtc cgtcagcacc tgcatcacgc
     1681 ttactggcgg cggggcgtcg gtgtagacgg catctaccat gctgatgctg cccatattga
     1741 ccatcgcgtt acggccaaat ttcgagtgat cgacaaccag cataacgtgg cgcgagttct
     1801 caataatggc gcgtttggtg cgaacttcgt ggtaatcgaa ctccagcagc gagccgtcgc
     1861 tatcgatgcc gcttatcccc agaatgccga aatcaaggcg gaactgggag ataaaatcga
     1921 gcgtcgcttc gccaatgatc ccgccatcgc ggctgcgtaa ttcgccaccg gcgagaataa
     1981 tgcggaaatc ttcttttacc atcaacgtgt tagcaacatt gagattgttg gtgacaatac
     2041 gcaaattgct gtgattaagc agcgcgtgcg ccaccgcttc cggcgtggtg ccgatatcga
     2101 taaacagtgt cgagccattg gggatttgct ccgccacttt gcgggcaatc ctctcttttt
     2161 cttcggtctg ggtggcttta cgatcgtgcc acggcgtgtt aaccgaactg gaaggcagcg
     2221 ccgcaccgcc atggtgacgc aggatcaggt tttgctccgc cagctcattg aggtcgcggc
     2281 gaatagtctg cgggctgacg gagaaatgct ctaccagctc ttcagtactg acataaccct
     2341 gctgtttaac cagttcgata ataccgttgt gacgttgtgt ttgtttcatt tataaatccc
     2401 tggaattatt ttcgttttcg cgcattgagc gaatcaacaa aagccatcgc taaacccacg
     2461 gctaacccgg cgatgtgtgc tccgttcgcc atcgacatcc caaacaaatc aaaccatccg
     2521 gcgacaatcc agatcagcgc aaagataatt aacccacgtt gcaggtaaat gccactttgc
     2581 ggatcgcgtt cgccacgtag ccagacgtag cccatcagcg catacaccac gccagaaagc
     2641 ccgccaaacc acggcccgct gaatttttgc tgcacatagc cgcttaacag ggcgctaatg
     2701 agagtaataa caattagctt accgctaccg aggcgttttt ccaccgcacc gccgagatac
     2761 caccaccaga gcaggttaaa gaggatatgc atcagcgaga agtgcattaa cgcgtgggtg
     2821 aagtaacgcc agaactcaaa tttcagtgtt ggatcgaatg gccaggccag ccataacatc
     2881 acttcctgat cgccgagaat ttgcatggcg ataaacacca ccacgcaggc gatcatcacc
     2941 acccaggtta ccggacctgc gcgttcacgc aaggcagcaa agaaaggata acggcgataa
     3001 tgcagaccac tgccggtatg gcccgcctgc cagctggccg ccagataacg cggatctgcc
     3061 gggttttcga gaaaacgcgc cagctccgcc cgcacgcgct cggcctggga ctcatccgcc
     3121 agccagacat cgctttggtt atgttgttga atcgtgagga taacaccctg cgtcgccata
     3181 taatcaacaa acgcctgcgc cacgcggggg ttagcaaaag aggtaatcat caacatcgtt
     3241 gctgtcgctt attccacaca aaaggggaca gtataaagcg ttacgcgccg tacgccacct
     3301 ctgcgggaaa ctgacgctgc caggcttcaa aaccgccgtc aatgctatag accacatcgt
     3361 agccctgttg cagcagatac tgcgccgcgc ctttgctgct attgccgtga taacacatca
     3421 ccatcaccgg agtgtcaaag tcgttatcac gcataaacgc gcccagcgtg tcgttggtta
     3481 aatggaaagc ctgcaccgca tgccccatag cgaaactctg tggatcgcga atatcgacca
     3541 gcaccgcctc tttttcctgc aacttctggt gcgcgtcggc aacgttaata cattcgaaca
     3601 gatccatgcg tctctctttc tttacaaaca agtgggcaaa tttaccgcac agtttacgtc
     3661 gaagcggcag ataaacgcca taatgttata catatcactc taaaatgttt tttcaatgtt
     3721 acctaaagcg cgattctttg ctaatatgtt cgataacgaa catttatgag ctttaacgaa
     3781 agtgaatgag ggcagcatgg aaaccaaaga tctgattgtg ataggtggcg gcatcaatgg
     3841 tgctggtatc gcggcagacg ccgctggacg cggtttatcc gtgctgatgc tggaggcgca
     3901 ggatctcgct tgtgcgacct cttccgccag ttcaaaactc attcacggtg gcctgcgcta
     3961 ccttgagcac tatgaattcc gcctggtcag cgaggcgctg gctgaacgtg aagtgctgct
     4021 gaaaatggct ccgcatatcg ccttcccgat gcgttttcgc ctgccacatc gtccgcatct
     4081 gcgcccggcg tggatgattc ggattggtct gtttatgtac gatcatctgg gtaaacgcac
     4141 cagcctgccg ggatcaaccg gtttgcgttt tggcgcaaac tcagtgttaa aaccggaaat
     4201 taagcgcgga ttcgaatatt ctgactgttg ggtagacgac gcccgtctgg tactcgccaa
     4261 cgcccagatg gtggtgcgta aaggcggcga agtgcttact cggactcgcg ccacctctgc
     4321 tcgccgcgaa aacggcctgt ggattgtgga agcggaagat atcgataccg gcaaaaaata
     4381 tagctggcaa gcgcgcggcc tggttaacgc caccggcccg tgggtgaaac agttcttcga
     4441 cgaggggatg catctgcctt cgccttatgg cattcgcctg atcaaaggca gccatattgt
     4501 ggtgccgcgt gtgcataccc agaagcaggc ctacattctg caaaacgaag ataaacgtat
     4561 tgtgttcgtg atcccgtgga tggacgaatt ttccatcatc ggcaccaccg acgtagagta
     4621 taaaggcgat ccgaaagcgg tgaagattga agagagtgaa atcaactatc tgctgaaagt
     4681 gtataacgcg cactttaaaa aacagcttag ccgtgacgat atcgtctgga cctactccgg
     4741 tgtgcgtccg ctgtgtgatg atgagtccga ctcgccgcag gctattaccc gtgattacac
     4801 ccttgatatc catgatgaaa acggcaaagc accgctgctg tcggtattcg gcggtaagct
     4861 gaccacctac cgaaaactgg cagaacatgc gctggaaaaa ctgacgccgt attatcaggg
     4921 tattggcccg gcgtggacga aagagagcgt gctgccgggt ggtgccattg aaggcgaccg
     4981 cgacgattac gcagctcgtc tgcgccgtcg ctatccattc ctgactgaat cgctggcgcg
     5041 tcattacgca cgcacttacg gcagcaacag cgagctgctg ctcggcaatg cgggggcgat
     5101 aagcgacctc ggggaggatt tcggtcatga gttctacgaa gcggaactga aatacctggt
     5161 ggatcacgaa tgggtccgcc gcaccgacga tgccctttgg cgtcgcacaa aacaaggcat
     5221 gtggctgaat gcggatcaac aatctcgtgt gagtcagtgg ctggtggaat atacgcagca
     5281 gaagttgtcg ctggcgtcgt aaggatgatg cccggttgaa gtgcaaccgg gctatttgga
     5341 ttccttagac tattcattgt aaataattga ataacggccc ctgagctaaa atcgccaaac
     5401 ttaagcacgt tacccaatcg ccccacttct tcctctggtt tctatttttt ctgcaccaat
     5461 ggcaataata tccccatgca aaaccaagct gtgcactaat cgggccgaca aaatcagtcc
     5521 aaggctgcgg ccaaaaaggg gaaaaattca ggggaggcca gacaaacgtc agaaagaaat
     5581 ttcggatatt ttccagcaac gctgatttct ctggagaaat ataaagaatg aaattcgaga
     5641 gaattaacca gaaatagacc ggcgaaaaga ccagccagca atgcagtctg ttttttaatt
     5701 caggaggcgt tctgcgggag aattgccaac cgatgccagc cattacactg aaaaaaagtg
     5761 catattcaac cattaactca agttgtaatt cctgaagaga aatacgcaca accaggagcg
     5821 gaaaggagaa tattatttct gccagaaaag ttaacgtgaa tataagtacg gctaagacaa
     5881 taaccgtcgg ccataccaat aatggcacga taacaaaacc gagaattaca gggggaatat
     5941 aaagccggaa tttaatcatt tttattttcc tggtgattcg gatgatgcgt catacatcgc
     6001 gcataaacac tcatcaatta tccagtcatc ttcccagacc tgatattctg tcattggttc
     6061 gggctgtttt agcgggttct caatcgcttg ccgcgctctt tctttcagta aaggatgatt
     6121 atttctagcc caggcattga tttgggtggt aatggcgtta tcgatatcgt cctggttatc
     6181 tctgatataa tcctcacaat atctgttcaa taaacaggca agagaaacgg tcgaccaaaa
     6241 gccgggaact gaattaatgg catccagcaa tggcgcaaga gcgatatcgg cacggaaaaa
     6301 catttccagc atatactgta attcgacagc ttcatcgccg gatacccaat cagtcatata
     6361 ctggcaggca aatcgctgta aaattgcccg ttcatccgca cgccagtttt ctttgttggc
     6421 aaggttgata cggcgcagag aaaaaactac gtgaatattg tcaaaatgcc cctgtgcaac
     6481 aaattccagt aaacgcggca gaaaatagcg gacctcatcg ctattttgcg gatcaggccc
     6541 tgggctacta ttccaggcat tgattaagga atacgggatc gctcgtaacg atgtactccg
     6601 taatgccttt tgttcctgct gactcaggca atattcacaa caaaccacaa atttctgcgg
     6661 cacccgataa cgacgaaagg tgtgataaat ctgctcaatt aatgcctgca tggcggggca
     6721 gggttgcata gttaccccct cgcccgctga tcgcgtgtaa acggttgata gacaaaacga
     6781 gcattcgccg cgttcaggcg aataagggcg ctacgcgaaa gggaaaagct ggggcgaatg
     6841 caactattgc agtctcgaca gctttcctct gcgcaatttt ccactgagta atgacgcgtg
     6901 tagagtttca gttcggcctg cttatcgcga aatccgcgta aactgttatt gttatgaaat
     6961 ttccaggcat ctttaaagac ctgaaagata tagacatcct caatccagga gtcacatttt
     7021 ttaagcgggc gctttgctat tatcacccgc tcatccggat ttagcatcgc cgtaaattgt
     7081 acggtgacgt catttgtcgt gttcaggtca cattgaacat ctggtacatc actgatatag
     7141 gggtcaaatc caaaatcagc accacaggca caaagacggc taatggtggc ggtatcgagg
     7201 gaaaatcccg gccatacctt gctggaggtt aagccaatat ttaatgtgac atcatatcga
     7261 agcaatccct caagaccgtc agcaccgacg aacttttccc cttcatccag ggcctggtta
     7321 ataagttgct gaacaatgcc ttcatcccag tatgccgatt tgtagttggc aatcgtaata
     7381 tccacgcctg tgaagggatc agtaccggca aggcttgcaa ataatgtatt aactaattcc
     7441 atttttatta tccagcttta aggtagcgtc ttatacatcg ggacataacc tacatcttcc
     7501 accaaactct gtccctgcgg cgttaaaaac cactccaaca atttttgtgt ctctgacgtc
     7561 gtattttccc gcgttatcat aaaggcatcg acgatatagg gatatttgcc gttgcgaatg
     7621 ttttcggcag tcggtgcaat accgttaatc gctagcagtt ttatattttt atcggcgttc
     7681 atttgtgttg cgtaataacg gaaggtgtaa cctattgcgt tgtttgtatt acggtattcg
     7741 gcaacgactt taatcattcc ctccatcata cttgccactt ctgtttcctg cggcgagatc
     7801 atgcggacat ttttcatgac ctgggattgc atcaccgtct ggctaccaga gtcttccggg
     7861 cgttgccagg tctggatctc ctgatcgtta ccgccaacag tacgccagtt ggtaattgca
     7921 ccactgaaga tgtcacgcac ctgttgttca gtcagggaat taaccggatt atccgcattg
     7981 acgatgaaaa caaacgcttc acgggcaaaa ggcgtgtaca gcaaagtaac gcccgattcc
     8041 tcagcgcgtt ttttctgccc accggaaggt tgcgccacaa aaataatatc agcatcacct
     8101 ttaacaatcc tgttatacgc atcgggggta cgagagctct ccagatattc cctggtgtga
     8161 aaatcttccg gtataacgct taaagcataa aatgcagaag cataaattgg gtacgccgcc
     8221 gtagcaccat cgagtcgcgg ccagttttgc gtaaactgaa tttgcggttt accacgcagt
     8281 ggtgtcagtt gattattgag tttatcgggt cgccaggccc aggtatcaat attttcgcgc
     8341 acaccgacgc cgggattgag ctgtttatat aaataaccct gataaccagc gacaaaacca
     8401 agcatgagaa ggataaacag gagggaatta cggcaacgta gcccggatgt accggttaaa
     8461 ggatgtctgg ttggccggcc gtaccccagc gaaaaactga tatgcgcgcc aacgggtata
     8521 atgactatcc accagtactc acctccgact actgagacta acaaaacgcc cagccagggg
     8581 agcataagat cagccaacca acccgatccc atctggtgaa attgataatc tgccagatgc
     8641 agcgcgatta tccagccaac caggattaat aaaccgggta ctaaaaaaag acattgtcgc
     8701 cagacggcta tttctctttt ttctggtgca ctatcgcctg cccatactcg ccccgagaaa
     8761 aatagcatta atgcgcccag cgccagcata aaaatgttga gcagaaggta acttgttggc
     8821 gtgataagga tattacaaac ttcaataata ccggcaagtc cgacacccag catagcaaca
     8881 accgccgcca aaaattgcgt cagtatgggg atgccgaaaa aagtcattac cagcgaggac
     8941 aaaatccatt ttctgttttg cattattctt tccattcttt ttgaatggtg aaattatact
     9001 ccccgagtcc ccttgcccct tctggacact tttccgaaat gatggcggaa aaaaacggga
     9061 gcctttggcc ccgttctatt tattggtgaa cttacaatct caccggatcg atatgccaga
     9121 tatgatcggc gtactctttg atagtacggt cagaagagaa gtagcccata ttggcaatgt
     9181 tcagcatcgc ttttgcggtc cactcttcct gaagctcgta gagttcgtcg actttatcct
     9241 gacaatcgac atagctgcga taatccgcca gaacctgata gtgatcgccg aagttgatca
     9301 gcgaatcaac cagatcgcga tagcgacccg gatcttccgg actgaataca ccgctgccga
     9361 tttgcgtcag cacctgatgc agctcctcat ctttctcgta gtattcacgc ggtttgtagc
     9421 cctgacgacg cagttcttcc acttcttccg cagtgttacc aaagataaag atattgtcag
     9481 caccgacatg atccagcatc tcgacgttcg caccgtccag cgtaccgata gtcagcgcgc
     9541 cgttaagcgc aaacttcatg ttactggtgc cggaagcttc cgtccctgcc agcgaaatct
     9601 gttcagacag atctgccgcc ggaatgatca actgcgccag gctaacgctg tagttcggga
     9661 tgaacacgac tttcagctta tcgccaatct gcggatcgtt gttgatcact ttcgctacgt
     9721 cattgatcaa atgaataatg tgcttcgcca tgtaataggc cgaagccgcc ttaccgccaa
     9781 aaatattcac gcgcggtacc cacttcgcat ccgggtcggc cttgatgcgg ttatagcggg
     9841 taatcacatg caacacattc atcaattgac gtttgtattc gtgaatacgt ttgatttgta
     9901 catcgaacaa cgcctttgga ttcaccacca cattcagctg ctgggcgata tactctgcca
     9961 gacgcttttt gttctccagc ttcgcctgat gcacagcgtg attaaccatt gggaaatcac
    10021 agtgttgttg cagctcatta agcaggctaa ggtcggtgcg ccagttacgg cccaggtgtt
    10081 cgtccagcac ggctgaaagc gatgggttcg ctaccgctag ccagcgacgc ggcgtcacac
    10141 cgttggtgac gttggtgaaa cgacccggga agattttcgc aaagtcggca aacaacgatt
    10201 gcaccatcag attagagtgc agttccgata caccgttaac tttgtggctc acaacaaccg
    10261 ccagccaggc catacgcaca cgacgaccgt tggattcatc aatgatcgac gcccgtccca
    10321 gcagatcggt atcgttcgga tactgttcct gcaaggtttt caggaaatag tcgttgattt
    10381 caaagatgat ctgcaggtga cgcggcagaa ttttacccag catatcaacc ggccaggttt
    10441 ccagcgcctc gctcatcagc gtatggttag tgtaggagaa gacctgacaa cacacctcaa
    10501 acgcgtcgtc ccagctaaat tggtgctcat cgatcagcag acgcatcatc tcaggaatcg
    10561 acagtaccgg atgggtatca ttgagatgaa tcgcgatttt atccgccagg ttatcgtagg
    10621 ttttatgcaa ctgataatgg cggcttaaaa tgtcctgaat ggtcgaggaa accaggaagt
    10681 attcctgacg caggcgcagc tcacgcccgg agtaggtgga gtcatccgga tacagtacgc
    10741 gagatacgtt ctcggagtgg tttttatctt ccactgccgc gaagtagtca ccctggttga
    10801 atttaccgag gttaatttcg ctactggctt gcgcactcca caaacgcaac gtgttggtcg
    10861 cgtcggtgtc gtaaccaggg attatctgat cgtaagcgac tcccagaatc tcttcggttt
    10921 caatccagcg cgttttttta ccttcctgct gaatgcgacc accaaaacgg actttatagc
    10981 gcgtgttgtg gcgtttgaat tcccacgggt taccgtattc cagccagtag tctggcgact
    11041 ctttctggct accgttaacg atgttctgct tgaacatacc gtagtcatag cggatgccgt
    11101 aaccgcgccc cggcaaccct aacgtcgcca gagaatcaag gaagcaagcc gccagacgtc
    11161 ccaggccacc gttaccgagg cctgggtcat tttcttcatc aatcagctct tcgagattta
    11221 accccatcgc ttccagtgcg ccctgtacat cttcgtaaat tcctagcgac aacatggcgt
    11281 tggagagcgt acggccaatc aaaaactcca tcgacaggta gtaaacctga cgagtttctt
    11341 gcgacaactg ggcacggttt gaacgtaacc agcgctccac gagacgatcg cgcacagcaa
    11401 ataacgttgc gttcagccat tcatgtttat tggcgacgac cgggtccttt ccaatcgtaa
    11461 acatcagctt gtaagcgata gagtgcttaa gagcttctac gctaagcgtg ggcgatgaat
    11521 atgtaaacgg agcattcata taggcgtttc ctgaaaacta tttcaagcga tagtaaagct
    11581 cacggtacga cttcgccgcg acctgccagc taaaatccat tgccatagcc tgacgttgca
    11641 caaaccgcca cagtgaagga cgggaccaca gtacaaaagc acgtcgaata gcccgtaaca
    11701 gcgaccaggc attactatct tcaaagacaa acccactggc gacgccatct gcaaggttct
    11761 caagagaaca gtcagaaacc gtatcagcaa gcccaccggt gcgccgcact aacggcagcg
    11821 taccgtactt caatccataa agttgcgtta agccgcacgg ttcaaaacgg ctgggcacca
    11881 gaatgacgtc cgcgccgccc ataatgcgat gcgaaaatgc ttcgtgatag ccaatctgaa
    11941 cgcccacctg gccggggtat tccgctgccg ccgcaaggaa accttcctgc agcaccggat
    12001 cgcccgcgcc gagtagcgcc agctgcccgc cctgctccag aaggcccggt aaggcttcca
    12061 gcaccaggtc gagacctttc tggctggtca gacggctcac cactgcaaaa agcggcactt
    12121 tatcatcaac cttaagcccc attgcgattt gtaactggcg cttattttcc gctttatctt
    12181 ccaacgtatc gcgggtgtag cgcgaggcca acagtaagtc cgtctctgga ctccagattt
    12241 tctcgtccac gccgttcagt acgccggaaa gacgcccttc acggtgacgc tgttgcaaca
    12301 gaccttccat accgtaggca aactgcggtt cggtgatctc gcgagcgtag gttggactaa
    12361 ccgccgtaat gtgatcggca tagtacagac cggccttcag gaaagagatt tgtccgttga
    12421 attccagccc atgaatatta aagaatgacc atggcaattg gatgtcattc atatgatgtg
    12481 cataaaacat gccttgatag gccaggttgt gcacagtaaa caccgacttc gccggacgcc
    12541 cgcgcgccgc cagatacgca ggcgcaaggc ctgcatgcca gtcgtgcgca tgcaccacat
    12601 caggacgcca gaatgggtca agcccgctgg ccatttctgc cccaacccac cccagcagcg
    12661 caaaacgcaa tacgttgtcg gtataggcaa ataagttggt atcgtgatac gggcttcccg
    12721 gacgatcata gagatgcggc gcgtcaatca ggtatatgcc aaccccgttg taatgaccga
    12781 acaacagcgt gatatgtccg gcgaaggtat cacgacggga tactacctgc gcatcggtaa
    12841 cgccacggcg aatatcggga aatgcaggca acagtacgcg agcgtcaacg ccgtctgcga
    12901 tttgtgctgc gggtaatgcc ccaataacat cagccagacc gccggtttta agcagcggga
    12961 acatctctga acatacatgt aaaacctgca ttatcgctcc tgtttatgcc ctaacttccg
    13021 tagcatttcg cgcgttacca gcacgatgcc ttcttctgaa cgatagaaac gacgtgcatc
    13081 ttcctctgcg ttttcaccaa tcaccatgcc ttccggaata acacaagcac gatcgatgac
    13141 gcagcggcgc agacggcacg agcgacctac ccatacttcc ggtaacaata cggcggaatc
    13201 aatgttgcag aatgaattca cgcgaacgcg cgagaacaga acggactgca ccaccaccga
    13261 accggagatc acacagccgc cggataccag tgagttaagg gtcatcccgt ggctaccgga
    13321 gcgatcctgc acgaatttcg ctggcggtaa tgattcattg taggtgcgaa ttggccaatt
    13381 gcgatcgtac atatccagtt ccggcaccac agaggccaga tcgaggttcg ctttccagta
    13441 agcttccagc gtacccacat cgcgccagta cggctcggca tccgggtcgg attgtacgca
    13501 agagagcggg aacgggtgcg cataggccag accggcttcg gtgatcttgg gaatcaaatc
    13561 tttgccaaag tcgtggctgg agttctcatc acgatcgtct tcttccagca gttcatacag
    13621 atagtcggcg tcaaagacgt agatacccat actcgccaga gatttgctcg gatcgttcgg
    13681 cattgacggt gggttagcag gtttttcaac gaattcgata attttatcgt tctcatcaac
    13741 cgccataacg ccaaatgcgg aggcttcttc aatcggtact ggcatacaag caacggtgca
    13801 acgtgcgcct ttttcgacgt gatcgataag catacgcgag tagtcttgct tgtagatatg
    13861 gtcgcccgcc aggatcacca cgtattccgc tttataacgg cggataatgt cgaggttttg
    13921 ggtgaccgca tctgcggtgc cgcgatacca gttttcccct ttcattctct gctgtgctgg
    13981 cagcagatcg acaaactcgt tcatttcttc attgaagaat gaccagccgc gctgaatgtg
    14041 ctgcaccaga gtgtgggact ggtactgggt gatcacgccc atacgacgga tcccggagtt
    14101 gatgcagtta gacagcgcaa agtcgataat gcggaactta ccgccgaagt gtacggccgg
    14161 ttttgctcgc ttattggtta aatccttcag gcgggtacca cgtcctcccg ccagtatcag
    14221 ggcaacagat ttcaatggca gctggcgcgc caacattaag tgatcgttct tctctaaact
    14281 aaccatgact aactcctttt ttatcatctc tggaacacac acaatccgtg tgcaggtccc
    14341 tgccagacag ccgtaatcac tgggttatcc tctccagcga atgggggaat ggcgtgccac
    14401 tccccagcag gtaaaacaat ctctgttacc tcaagcgtgg cgttaattgc gatcaaaaag
    14461 cgatccgaga gcagaatttg cagctgtttc ggcccgtttt gccactcatc cgtgcttaaa
    14521 ggttgagcat atcgatttag ccaacggaca ttgccgtcgc cttcttccca ccagcgattc
    14581 tccaccaaag cgggaatgcg cttgcgcaga tggattaacg cggcggtaaa tgcggttaaa
    14641 ccactgcttg cctgcgacca gtccaaccag gttaattggt tatcctgaca gtaggcattg
    14701 ttattgccat gctggctgtg accatgttcg tcaccggcca gtaacatcgg cgtaccctgg
    14761 gagagcaaca acgttgttaa cagggcgtga atgctgtcgc gccgccgttc aaccaggtca
    14821 agagaaccgc ctaacccttc tttaccatga ttgttactgt aattgttgtt ggtcccgtcg
    14881 cgattttctt ctccgtttgc ttcattgtgt ttatggttga agcaaacgca gtcgcgaagc
    14941 gtaaaaccgt catgcgcggt gacgagatta atcgcggcac tcggcagacg accattacgt
    15001 ttaaaaacat cgctggaggc agcaaaacgc ccggcaaacg cccccagagg caaatcataa
    15061 tgtagccaga aacgacgggc agcatcgcgg aaatgatcgt tccactcggc aaacagcggc
    15121 gggaaatttc ccacctgata accaccagga gcgatatccc acggttcagc aattaacttc
    15181 acctgcgaga gcaccgggca gttctggata gcggtaaaca acggcgcatc ctgacggaac
    15241 tctggcgtac ggcccatgac tgccgccaga tcaaagcgga aaccatcgac gtggcaggtt
    15301 tctacccaat aacgcaggca ggcgctggca taatccacca ccgccggatg actcaaattg
    15361 agcgtgttgc cgcaaccggt ccagttgtga taatcgccgt cttctcttat ccaataatag
    15421 ctacggttat cgatcccacg cagcgagaat aacgggccgt cgaggtccag ttccgcacta
    15481 tggttgagca cgatatcaag aatgacttcg atacccgctt tatgcagtgc tttgattgca
    15541 tcgcgaaact catccagcgc cgtttctggc gagcaggcat acgccggatg cagcgcaaac
    15601 atcgccaccg ggttgtaacc ccagtagtta cttagcccca tgcgttgcag acgtggttca
    15661 ctggcaaact gcgccactgg cagcagttcc agcgcggtaa tgcccaattg tttcaaatag
    15721 ttgatcatca ccggatgccc gagggcttta taagtgccac ggatctcgac cgggatctcc
    15781 gggtgcaagt acgttaatcc tttgacatgg gcttcataaa tgatggtgct gccccacggc
    15841 gtgcgcggcg gggcatcatc ttcccagtca tagtgatcaa ccaccactac gcatttcggc
    15901 gcaatggcgg cgttgtcgcg atagtcaggt tcattatgac cggcgtgcag cagcgggtta
    15961 tctttaaact ccccgtcaat ttgccgcgcg caaggatcaa tcaacaactt cgccgggtta
    16021 aagcgatgcc cctcggcggg ttgccagggg ccatgaacgc gataaccata acgcaaaccc
    16081 gggcgcgcat ccggcagata accgtgccaa atgtcgccac tgtgccctgg caagtcatag
    16141 cgatgttcct ggccattggc gtcaaagaca cacagttcta cccgctcggc atgagcggag
    16201 aaaagtgtga agttgacgcc ctgaccgtcg taatgcgcgc cgaggggagc gggtttgcca
    16261 atggcgagtt gtgtcattct gcctcccgaa ccagccagat agtggccagc ggtggtagcg
    16321 tcaggcttag tgaatgctga cgaccgtggc tggcaatctc atcgctgtgt accgtgccgc
    16381 cattgcctgc attactgccg tgatagtgca tggaatcggt attgaggatt tcacgccatt
    16441 tgcccggctg gtttatgccg aagcgataat catgacgcgg taccggcgta aagttactgg
    16501 caacgatgat ttcgttaccc tctttatcgc gacgcacaaa gatcagcacc gagcgttctt
    16561 tgtcatccac caccagccat tcaaagccgt acgggtcaaa atccagttca tgcattgctt
    16621 tatggtggcg gtaggtgagg ttcagatcgc gcaccagacg ctggacaccg tggtgccagt
    16681 tatcgccgcc ttccaacaga tgccagtcga ggctggcgtc atggttccac tcgcggccct
    16741 gggcaaattc gttacccatg aacagtagtt tcttgcccgg gaatgcccac atccagccat
    16801 agtaggcgcg caggttcgcg aatttctgcc atgcgtcgcc cggcatgcgg tcgagaatcg
    16861 attttttacc gtggaccact tcatcatgcg acaacggcag gacgaagttt tcagtgtagt
    16921 tgtagagaat cccgaaggtc agtttatcgt gatgatactg acgataaacc gggtcgagct
    16981 tcatgtagtc cagggtgtca tgcatccagc cgaggttcca cttgtaccag aagcccagac
    17041 cgcccatatc ctgcggacga gaaacgccag ggaaatcggt agactcctca gccattgtca
    17101 ccgcaccgga aacctgctca ccaagaatac ggttggtatt acgcaagaat tcaatcgctt
    17161 caagattctc gcgcccgcca aattcgttcg ggatccactc cccctcttta cggctgtagt
    17221 cgcgataaat cattgacgcc accgcatcga cgcgcagcgc atcaatacca aaacgttcaa
    17281 tccagtaaag cgcgttaccg acgaggaagt tactgacttc acggcgacca tagttgtaga
    17341 tcagcgtgtt ccagtcctga tgatagcctt cacgcggatc gctgtgttca tacaagttcg
    17401 tgccatcaaa ttcggcaagc gcaaagtcat cagtcgggaa gtggcctggc acccagtcga
    17461 gaatcacgtt cagaccagct gcgtgtgcgg catcaatgaa ataacggaag tcgtcgcgag
    17521 taccaaaacg gcgggttggt gcatacaggc cggttggctg ataaccccaa ctgccatcga
    17581 agggatgctc gttaatgggc agtagttcga ggtgggtaaa gcccatccat ttagcataag
    17641 gcaccagttg atcggccagc tcgcggtagc tcaaccagaa attgttgtcg gtgtggcgac
    17701 gccaggaacc caggtgaact tcataaatag agattggcgc atcaaactga ttcgcttttt
    17761 tgcgctcttc agtctgtaca accttttccg gcagcccgca aataagagac gcggtttccg
    17821 ggcgcatttg cgcttcgaag gcataagggt cggacttcag acgcaagttg ccattggcat
    17881 caatcatctc gtatttatag agctgaccgt tatgcgcccc agggataaac agttcccaga
    17941 tgccgctctc tttacgcagg cgcatcgggt gacggcgacc gtcccagtag ttgaattgcc
    18001 caaccaccga gacccgacgg gcgtttggag cccagacaga gaaacgcgta cctgtgacgc
    18061 catccatagt atctgcatgc gcgcctaagg tttcatacgg gcgcaggtga gtaccttcag
    18121 ataatagcca ggcatccatt tcctggatta gcggaccaaa acggtaagga tcatcaatca
    18181 ggttttgctg accatgccag acaacagcca actgatagcg gaaaaaattc ttacgtcgcg
    18241 gaatgacgcc gctaaagaat ccccgtgagt cgagacactc cagttttgcg agtttgcgcc
    18301 cggttttcgg ttcaatcacc cacacatcgg tagcgtcggg taaaagggca cggacttcca
    18361 gtcccgcggt ggttttatgc attcccagta cggaaaaagg atccgcaaaa tggcctgcaa
    18421 ttagcgcgtt aatcacgtct ctatcgatac gatcggacat gcttgtcttc ctgttttatt
    18481 gtgtcacccc atccaattgg atttttgact tctggttgtg acattttttt gacctgaacg
    18541 gcgcagcact ctgtgcatcc tctctgcgtc gtcctcactt caggtaaggc tgtgaatact
    18601 catgtattca gccaccctta aagaatagcc aatgctctat ttaactcccg gtaaatcatg
    18661 aaacatctgc gcttactcct gtattacgca ctaacagggg cggcatcgcg ccccagattt
    18721 aatgaataaa gattacgcca gttgacgaag catccgacgc agcggctccg cggcccccca
    18781 cagcagctgg tcgcccacgg taaaggctga caggaactct ggtcccatat tcagcttacg
    18841 caggcggcct accggcgtgg tcagcgtgcc ggtaacggca gctggggtta gctcacgcat
    18901 agtgatttcc cgatcgttcg gaacgacttt cgcccacgga ttgtgcgcag ccagcagttc
    18961 ttccacggtc ggaatagaca catctttttt caatttaata gtgaatgcct ggctgtggca
    19021 gcgcaatgcc ccgacacgca cacataaacc atctaccgga attacggaag atgtgttgag
    19081 gatcttgttg gtttccgcct gccctttcca ctcttcgcgg ctctgaccgt tatcgagctg
    19141 tttgtcgatc cacggaatca ggctacccgc cagcggcacg ccaaagttat ccaccggcag
    19201 ctcaccgcta cgggttaagg ttgtgacttt gcgttcgata tcgagaatag cagaggacgg
    19261 ggtcgcgagt tcatctgcca catggccata cagatggccc atctgggtta ataactcacg
    19321 catatgtcgc gcaccaccgc cggaagcggc ctggtaggtt gcaacggaca cccaatcaac
    19381 aagatcattg gcgaataaac cacccaacga catcaacatc aggcttacgg tacagttacc
    19441 gccaacaaaa gtcctgatgc cattatttaa tccgtcggta atgacgtcct gattgacggg
    19501 gtcaagaatg atgatggcgt catctttcat gcgcagagac gatgctgcgt caatccagta
    19561 accttgccat ccgctttcac gaagctttgg atagatttcg ttggtataat cgccgccctg
    19621 acaggtcaca atgatatcga gggcctttag cgcctccaga tcaaaggcat cctgaagtgt
    19681 gccagtggtt ccgccaaaag acggcgcagc ctggccaagc tgagaagtag aaaagaagac
    19741 agggcgaatg gcgtcgaagt cgcgctcttc aaccatgcgt tgcatgagaa cggagccgac
    19801 cataccgcgc cagccgataa aaccaacatt tttcataagc gttttttcct gcaaagatgt
    19861 gtgctgtata aatgtgccgg tctcctcttg gcacatcttt caccatacaa aaagcagcca
    19921 aagtcgcaag tgaaattaat caatgatagc gaagccatca gtaatgcgac ttatcctgct
    19981 ttgttagcac gcagaaagtc cgcggcaatt atcagggaat ttgagttatg aatgaaatca
    20041 tttctgcagc agttttattg atcctgatta tggatccgct cggaaaccta cctattttca
    20101 tgtccgtact gaaacatact gaaccgaaaa gacggcgggc aatcatggtg cgagagttgc
    20161 ttattgctct cctggtgatg ctggtgttcc tgtttgcggg tgagaaaatt ctggcatttc
    20221 tcagcctgcg agcagaaacc gtctccattt ctggcggcat cattctgttt ctaatcgcca
    20281 ttaagatgat tttccccagc gcttcaggaa atagcagcgg gcttccggca ggtgaagagc
    20341 catttatcgt gccgttggct attccgttag tcgccgggcc gactattctc gccacgctga
    20401 tgttgctgtc tcaccagtac ccaaatcaga tgggacatct ggtgattgct ctgctgctgg
    20461 cctggggcgg cacctttgtc atcctgctac agtcttcgct atttttacgt ctgctgggcg
    20521 agaaaggggt gaacgcactt gaacgcctga tgggattgat tctggtgatg atggcaaccc
    20581 agatgttcct cgacggcatt cgaatgtgga tgaaggggta acgaggagag tttttcgaaa
    20641 agaacccggt agcgtcacaa ctaccgggcg aacaaactta actcaacagc tggaacgcaa
    20701 tcatcccaac gatggcaccg acagtgccga ggatggtttc catcatggtc caggttttca
    20761 gcgtttcggc ttcggtcgcg ccggtaaatt taccgaacaa ccagaaaccg gcgtcgttaa
    20821 cgtggctgac aacaatcgaa ccaccagcga tacaaatcga cagcgccgcc atttgcgcac
    20881 cggagtagtt cagttgttca ataaccggca tcaccagtcc taccgccgtt aaacaggcta
    20941 cggtggcaga accctgaatg atgcgcactg cagcggccag cacgaagcag gtgatggcta
    21001 tcggcaggcc catgccagtt aacgcttcgc ccagtgccgg acctacgcca gagtcaacca
    21061 gcacctgttt gaacacgcca cctgcaccaa tcaccagcag aatgatcccc gccggttgca
    21121 gcgcgtgacc gcaaatctcc atcactttgt cttttggcat gccctgacgc attgccaggc
    21181 cataaatcgc taccagacaa gcaaccagaa tcgcggtaaa cggatgacca ataaactcga
    21241 accattcgta agcggtagag ccttctggca caaaacgcgc ggcaatggtt ttcagcccca
    21301 ccagcaccag cggcaacagg atcagcgaca ggctgaatcc gaaagatggc attttgcctt
    21361 cgccgagatg cggttcgctg atgtcgtcag gaatatgcaa ctccacgtag cggctgatga
    21421 aattacccca cagcggcccg gcaataatca ttcccggaat tgccgcacac aggccaatca
    21481 ggatcatcca gccaaaatcg gcgttcatct gcgatgccag cagcattggc gctggccctg
    21541 gcaccaggaa cgccgccgct gccgccacgc ctgcgaataa tgggattacc agcttcacca
    21601 ggttcgtacc ggtgtggcgc gccattgaga aagcaacgct aatcagcaga acaatcgcca
    21661 cttcaaagaa cagcggtagc gcacagacca gccccgcaag gccgatggca taatgcgcgc
    21721 ggctgtgacc gaaggattta agcattttga cggcaatctg atcgactgcg ccggtttcat
    21781 gtaagatctt gccaaacata gctcccaggg cgacaaccac cgccaggaag ccgagggtgc
    21841 ctcccatccc tttttccatc gtcgctgcga ttttatcgag cggcataccg gaaaaaaggc
    21901 cagcccccat ggacaccacc atcaaagcca ggaaagcgtg catacgcgcc ttcatgacta
    21961 aaaacagcag cagtaaaaca gaccctactg ctgttaaaac aagcgttaat gtagtcacta
    22021 cttatttgcc ttttttaata acctcaatgg tgcttgccac aacaccttcc agcggttgat
    22081 cgatatccac caccagtaca tcagtttcgt ccgcacccgg ctcctgcagc gtttcaaact
    22141 gcgtcaccaa catttgggtt ttaaagaaat ggcctttgcg cgctttcagg cggctttcaa
    22201 tcacgtcaaa atcgcctttc aggtagatga aagagagatt cggattacct tcacgcagca
    22261 agtcgcgata gtgttttttc aatgcagaac agacgatcag tgacacttta ttagtgcgct
    22321 gcatagcaaa cgcggcgtcg ttcagcgcct gcaaccacgg tttgcgatcg tcgtcattca
    22381 gcggttcgcc agacgccatt ttttcgatat tgcgccgtgg atggaggaaa tcgccatcaa
    22441 gaaacgcggc atgaagttga tgcgccactt cactggcgac cgcagatttg ccgctgcccg
    22501 atacgcccat caagacgtaa atgtggtgat catggttagt cgtgctcaaa gtggtgcccc
    22561 cacaatacaa gaattaaaaa tgttacgggt aactgttatc ggtaacattg tccagccgga
    22621 caatatcaga agcaatatcc atccgtgcct taagtgtata agtgtgagct acttcaaatt
    22681 tgtaggctta aatagatccg cccggtgaca aggtgaaacc taaatctaac attttcggtg
    22741 tcacagattc gccacgaata cgcgccagca ggcgttcagc gccaatactg cccatccgct
    22801 cacgcggcgt cagcacgctc gctaaccgcg gctccatcac ctggccaatg tcatgaccgt
    22861 ggaaaccggc aatcgccata tcgtcaggaa ccttcaatcc cagacgctga cattcaaacg
    22921 ccgcgccgac cgccaggtca tcgttggtac agaacacgcc atccagctgc ggatattccc
    22981 gccgcgcctg gcgaatcagt tcaataccgg aagagtaaga agaagattgc tcaaccatca
    23041 cgctatacgg caccagacct gcatccagca tcgcctgttc gtatcccttc tgtttgatga
    23101 tagtacgttc gtcgagacgc gcgccgagat aggcaatgtg gcaatgcccg cgagcaataa
    23161 tggcggtggt catctggcgt gccgcttcaa agttatcaaa accgacggcg atatcgaggc
    23221 acggcgactg gctgtccatg agttccacca ccgggatccc cgccacttca atcatcttta
    23281 aggtgcgcgg cgtgtgggta cgttcggtga ggatcagtcc gtcgatattc catgagagca
    23341 tggattcgag acgttcttgc tccatttccg gtttataacc gtagtgcgcc agcatggtct
    23401 gataaccgtg cgcgtcggtg acgctttcga ttccgcgtaa tacttccgcg aaaacctggt
    23461 tggtgagaga aggtaacagg acgccaatcg cccggctggt ggcgttagag aggatatcgg
    23521 gcgcacgatt gggaatatag cccagttcat caagagcggc ggcaatcttg ccgcgtagag
    23581 cgacggaaac ctgctccggg ttgcgtaaaa aacggctgac cgtcattttg gtcacgccta
    23641 cacggtcagc cacatcctga agtacgggtc ttttcttttt catcgtcctg aaggtacaaa
    23701 agagatagat tctcttagtt taacacggac gcatcagaac cttcccgggg aaaacagtgc
    23761 ttgtttacaa ttattagata tacactaaat caattgcatc gcattgtgct aacgacgcgg
    23821 caaaaccccg gaagcgtgca tcagcacgcg accggagcga gcgaggaagc ctgcaaaaag
    23881 gcaggcttca aaaatgccgt ggagctttaa accggcggca gatcgaacag taacacttcg
    23941 ctgtcgctat ccgcatggat ggagattgcc tgctcatccc agattgccag accatcgctg
    24001 gtcgaggctt tcacgccgtt aatggtgaca ttgcctttca ccacctggat ccagacgcgg
    24061 cgttcagcgg caatctgatg caccgactgc tcatctttca gcaacgccca gcggtacagt
    24121 tccatatcct gatgcacttt caatgaacca tctcgcgcat ccggcgagag caccagctgt
    24181 ttgccctgta cggcatcgaa gcgacgctgt tcataacgcg gcgtaatacc gttttcttca
    24241 ggcatgatcc agatctgata cagatgcaga cgctcggtgc tgcttgggtt gtactctgag
    24301 tgacgaatac ccgtacccgc actcataatc tggaactcac ccgccggaac ctgctcttta
    24361 ttgcccatgc tgtcctgatg ctcaacagta ccttccagca cgtaggtcaa aatttccata
    24421 tctttatgcg ggtgagtgcc gaagccctgc cctgcttcaa tcacgtcgtc gttaatcacg
    24481 cgcagcgcgg agaagcccat aaagttcgga tcgtaatagt tggcaaaaga gaaagtatgc
    24541 caggagtcca gccagccatg atttgcatga ccacgttcat ttgctttgcg taagtagatc
    24601 atttgtattc gccccctgaa tgatttcgat gggcttagtg tggacccgat ctgcctggga
    24661 tgatagaggg tgaaaattga cccctctgtt caaaaaaatt gaactattcg aggggccatc
    24721 aaacttaatt cgcgagagtc actgtggagg gagaggcttg ctcaaatccg cgttcaagga
    24781 tttccagatt ggtaagaact tcagattcct tgacgtaatt tggcgcaccg tgggtgatgg
    24841 tttgatacaa cgcatcataa acgcgcccgt aatcgcccat ctccggcttc atctcttctc
    24901 tgaccgtcac gccctcgtca ttgacatact ccagcacacc gaccgaatta tccgctgcga
    24961 atcccggttc gcccggcata atattagcct tcaggctggt ttcctgctgg tcgataccgt
    25021 atttaataaa cgaacctttc ttaccgtgaa cgataaattt cggataatcg attttcacca
    25081 gatggctggt tttgacgatg gctttcaggt cgccataaaa cagttgcgct tcaaaggtgt
    25141 cgtcaggatt ggctttatta cgcaggctgc ggatgtcata agcgacgtga tccgggcgac
    25201 cgaacagaga aataatctgg tccatcgtat gcacaccaag gccataaaac gcgccatcct
    25261 gcggcagccc aggtttggtt tctgccaccg ggcggtaata gtcaaaatgg ctttccactt
    25321 caacaatctc tcccaacttg ccactttcaa tcgctttttt cgctgtcagg aagcaggagt
    25381 caaagcgacg attctgatac ggcgtgacgg tcagcccttt gcttttcgcc aacgcaaaca
    25441 gctctttcgc ctgcgcaagt gtcggagtga acggtttttc gaccagcaca tttttcccgg
    25501 cttccagcgc gcgcttcgcg tactcgaagt ggctgtccgc gtgggtgcag acaacaacca
    25561 gcttaacatc gggatcgttt agtacttcgt cgagatcgct ggtgaaatgg atatgggaat
    25621 aaatgggagc ctgttcttcc ggcttcgcat ggcgacgaaa aatatgcgcg acatgccagc
    25681 tatccttgcg gttaagtaca tacggcagat ggtaacgggt ggtgcttttg ccgaagccaa
    25741 taaaggcgca gttgatgacc atgatttcgt cctttttaag gtggttattt acaccttagc
    25801 gcaaagcgga cgtggttcct actggagtgc gcataacgaa cacaagcact cccgtggata
    25861 aattgagaac gaaagatcaa aaaaaaagcc agcacccggc tggctaagta atactggaag
    25921 caatgtgagc aatgtcgtgc tttcaggttc tccgcgaggg tcttcctgat cgcgagacaa
    25981 taataatcat tctcattcgc acttgtccaa cactttttgc aaaaaaatgc atttgactcg
    26041 catttgaaag tcaatgatgt tgaaagggac atttacccca aagaggacaa aggaatgagt
    26101 gagatagtaa tacgccacgc agaaacacgg gattacgagg ccatcaggca gattcacgcc
    26161 cagccggagg tgtattgcaa cacactacag gtgcctcatc cttccgatca tatgtggcag
    26221 gagcgactcg ccgatcgtcc cggcatcaag caactcgtcg cctgtattga tggagacgtc
    26281 gtgggccatc tcaccattga cgtgcaacag cgcccacgcc gcagtcatgt tgccgatttt
    26341 ggtatctgtg tcgactctcg ttggaagaac cgcggcgtcg ccagcgccct gatgcgagag
    26401 atgattgaaa tgtgcgacaa ctggttgcgg gtagatcgca ttgaactaac cgtgtttgtc
    26461 gataacgccc cggcaattaa ggtctataaa aaatacggct ttgaaattga agggactggt
    26521 aagaagtacg cattgcgtaa tggtgaatat gtcgatgcat attatatggc gcgggtgaag
    26581 taagatagtg ccctttttct gagatggaaa aagggtgtca ttcaaaatcg acataccttc
    26641 ctttaaggta
//
LOCUS       DAZKHF020000037        26234 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2201S31241
            SAMN43267800-rid25106413.denovo.037, whole genome shotgun sequence.
ACCESSION   DAZKHF020000037 DAZKHF020000000
VERSION     DAZKHF020000037.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267800
            Sequence Read Archive: SRR30307263
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 26234)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 26234)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 26234)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350581.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350581.2
            Genome Coverage       :: 151x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:21:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,652
            CDSs (total)                      :: 5,557
            Genes (coding)                    :: 5,320
            CDSs (with protein)               :: 5,320
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 237
            CDSs (without protein)            :: 237
            Pseudo Genes (ambiguous residues) :: 0 of 237
            Pseudo Genes (frameshifted)       :: 102 of 237
            Pseudo Genes (incomplete)         :: 130 of 237
            Pseudo Genes (internal stop)      :: 52 of 237
            Pseudo Genes (multiple problems)  :: 41 of 237
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26234
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267800-rid25106413.denovo.037"
                     /serotype="O 26:H11"
                     /isolate="2201S31241"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-01"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            complement(<1..596)
                     /locus_tag="ACHOSC_RS06285"
                     /old_locus_tag="pgaptmp_001257"
     CDS             complement(<1..596)
                     /locus_tag="ACHOSC_RS06285"
                     /old_locus_tag="pgaptmp_001257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HGU4547233.1"
                     /translation="MSKEKFERTKPHVNVGTIGHVDHGKTTLTAAITTVLAKTYGGAA
                     RAFDQIDNAPEEKARGITINTSHVEYDTPTRHYAHVDCPGHADYVKNMITGAAQMDGA
                     ILVVAATDGPMPQTREHILLGRQVGVPYIIVFLNKCDMVDDEELLELVEMEVRELLSQ
                     YDFPGDDTPIVRGSALKALEGDAEWEAKILELAGFLDS"
     gene            complement(667..2781)
                     /gene="fusA"
                     /locus_tag="ACHOSC_RS06290"
                     /old_locus_tag="pgaptmp_001258"
     CDS             complement(667..2781)
                     /gene="fusA"
                     /locus_tag="ACHOSC_RS06290"
                     /old_locus_tag="pgaptmp_001258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020455498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HGU4547234.1"
                     /translation="MARTTPIARYRNIGISAHIDAGKTTTTERILFYTGVNHKIGEVH
                     DGAATMDWMEQEQERGITITSAATTAFWSGMAKQYEPHRINIIDTPGHVDFTIEVERS
                     MRVLDGAVMVYCAVGGVQPQSETVWRQANKYKVPRIAFVNKMDRMGANFLKVVNQIKT
                     RLGANPVPLQLAIGAEEHFTGVVDLVKMKAINWNDADQGVTFEYEDIPADMVELANEW
                     HQNLIESAAEASEELMEKYLGGEELTEAEIKGALRQRVLNNEIILVTCGSAFKNKGVQ
                     AMLDAVIDYLPSPVDVPAINGILDDGKDTPAERHASDDEPFSALAFKIATDPFVGNLT
                     FFRVYSGVVNSGDTVLNSVKAARERFGRIVQMHANKREEIKEVRAGDIAAAIGLKDVT
                     TGDTLCDPDAPIILERMEFPEPVISIAVEPKTKADQEKMGLALGRLAKEDPSFRVWTD
                     EESNQTIIAGMGELHLDIIVDRMKREFNVEANVGKPQVAYRETIRQKVTDVEGKHAKQ
                     SGGRGQYGHVVIDMYPLEPGSNPKGYEFINDIKGGVIPGEYIPAVDKGIQEQLKAGPL
                     AGYPVVDMGIRLHFGSYHDVDSSELAFKLAASIAFKEGFKKAKPVLLEPIMKVEVETP
                     EENTGDVIGDLSRRRGMLKGQESEVTGVKIHAEVPLSEMFGYATQLRSLTKGRASYTM
                     EFLKYDEAPSNVAQAVIEARGK"
     gene            complement(2878..3348)
                     /gene="rpsG"
                     /locus_tag="ACHOSC_RS06295"
                     /old_locus_tag="pgaptmp_001259"
     CDS             complement(2878..3348)
                     /gene="rpsG"
                     /locus_tag="ACHOSC_RS06295"
                     /old_locus_tag="pgaptmp_001259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001742319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S7"
                     /protein_id="HGU4547235.1"
                     /translation="MPRRRVIGQRKILPDPKFGSELLAKFVNILMVDGKKSTAESIVY
                     SALETLAQRSGKSELEAFEVALENVRPTVEVKSRRVGGSTYQVPVEVRPVRRNALAMR
                     WIVEAARKRGDKSMALRLANELSDAAENKGTAVKKREDVHRMAEANKAFAHYRW"
     gene            complement(3445..3819)
                     /gene="rpsL"
                     /locus_tag="ACHOSC_RS06300"
                     /old_locus_tag="pgaptmp_001260"
     CDS             complement(3445..3819)
                     /gene="rpsL"
                     /locus_tag="ACHOSC_RS06300"
                     /old_locus_tag="pgaptmp_001260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005306278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12"
                     /protein_id="HGU4547236.1"
                     /translation="MATVNQLVRKPRARKVAKSNVPALEACPQKRGVCTRVYTTTPKK
                     PNSALRKVCRVRLTNGFEVTSYIGGEGHNLQEHSVILIRGGRVKDLPGVRYHTVRGAL
                     DCSGVKDRKQARSKYGVKRPKA"
     gene            complement(3945..4232)
                     /gene="tusB"
                     /locus_tag="ACHOSC_RS06305"
                     /old_locus_tag="pgaptmp_001261"
     CDS             complement(3945..4232)
                     /gene="tusB"
                     /locus_tag="ACHOSC_RS06305"
                     /old_locus_tag="pgaptmp_001261"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0002143 - tRNA wobble position uridine
                     thiolation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase complex subunit TusB"
                     /protein_id="HGU4547237.1"
                     /translation="MLHTLHRSPWLTDFAALLRLLSEGDELLLLQDGVTAAVDGNRYL
                     ESLRNAPIKVYALNEDLIARGLTGQISNDIIPIDYTDFVRLTVKHSSQMAW"
     gene            complement(4240..4599)
                     /gene="tusC"
                     /locus_tag="ACHOSC_RS06310"
                     /old_locus_tag="pgaptmp_001262"
     CDS             complement(4240..4599)
                     /gene="tusC"
                     /locus_tag="ACHOSC_RS06310"
                     /old_locus_tag="pgaptmp_001262"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase complex subunit TusC"
                     /protein_id="HGU4547238.1"
                     /translation="MKRIAFVFSTAPHGTAAGREGLDALLATSALTDDLAVFFIADGV
                     FQLLSGQKPDAVLARDYIATFKLLGLYDIEQCWVCAASLRERGLDPQTPFVVEATPLE
                     ADALRRELANYDVILRF"
     gene            complement(4599..4985)
                     /gene="tusD"
                     /locus_tag="ACHOSC_RS06315"
                     /old_locus_tag="pgaptmp_001263"
     CDS             complement(4599..4985)
                     /gene="tusD"
                     /locus_tag="ACHOSC_RS06315"
                     /old_locus_tag="pgaptmp_001263"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016783 - sulfurtransferase activity
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase complex subunit TusD"
                     /protein_id="HGU4547239.1"
                     /translation="MRFAIVVTGPAYGTQQASSAFQFAQALIVEGHELSSVFFYREGV
                     YNANQLTSPASDEFDLVRGWQQLNAQHGVALNICVAAALRRGIVDETEAGRLGLASSN
                     LQPGFTLSGLGALAEASLTCDRVVQF"
     gene            complement(4985..5707)
                     /gene="yheO"
                     /locus_tag="ACHOSC_RS06320"
                     /old_locus_tag="pgaptmp_001264"
     CDS             complement(4985..5707)
                     /gene="yheO"
                     /locus_tag="ACHOSC_RS06320"
                     /old_locus_tag="pgaptmp_001264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709120.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0140110 - transcription regulator activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HGU4547240.1"
                     /translation="MSRSLLTNETSELDLLDQRPFDQTDFDILKSYEAVVDGLAMLIG
                     SHCEIVLHSLQDLKCSAIRIANGEHTGRKIGSPITDLALRMLHDMTGADSSVSKCYFT
                     RAKSGVLMKSLTIAIRNREQRVIGLLCINMNLDVPFSQIMSTFVPPETPDVGSSVNFA
                     SSVEDLVTQTLEFTIEEVNADRNVSNNAKNRQIVLNLYEKGIFDIKDAINQVADRLNI
                     SKHTVYLYIRQFKSGDFQGQDK"
     gene            complement(5874..6686)
                     /gene="fkpA"
                     /locus_tag="ACHOSC_RS06325"
                     /old_locus_tag="pgaptmp_001265"
     CDS             complement(5874..6686)
                     /gene="fkpA"
                     /locus_tag="ACHOSC_RS06325"
                     /old_locus_tag="pgaptmp_001265"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FKBP-type peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HGU4547241.1"
                     /translation="MKSLFKVTLLATTMAVALHAPITFAAEAAKPATTADSKAAFKND
                     DQKSAYALGASLGRYMENSLKEQEKLGIKLDKDQLIAGVQDAFADKSKLSDQEIEQTL
                     QAFEARVKSSAQAKMEKDAADNEAKGKEYREKFAKEKGVKTSSTGLVYQVVEAGKGEA
                     PKDSDTVVVNYKGTLIDGKEFDNSYTRGEPLSFRLDGVIPGWTEGLKNIKKGGKIKLV
                     IPPELAYGKAGVPGIPPNSTLVFDVELLDVKPAPKADAKPEADAKAADSAKK"
     gene            6907..7125
                     /gene="slyX"
                     /locus_tag="ACHOSC_RS06330"
                     /old_locus_tag="pgaptmp_001266"
     CDS             6907..7125
                     /gene="slyX"
                     /locus_tag="ACHOSC_RS06330"
                     /old_locus_tag="pgaptmp_001266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein SlyX"
                     /protein_id="HGU4547242.1"
                     /translation="MQDLSLEARLAELESRLAFQEITIEELNVTVTAHEMEMAKLRDH
                     LRLLTEKLKASQPSNIASQAEETPPPHY"
     gene            complement(7174..7764)
                     /gene="slyD"
                     /locus_tag="ACHOSC_RS06335"
                     /old_locus_tag="pgaptmp_001267"
     CDS             complement(7174..7764)
                     /gene="slyD"
                     /locus_tag="ACHOSC_RS06335"
                     /old_locus_tag="pgaptmp_001267"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HGU4547243.1"
                     /translation="MKVAKDLVVSLAYQVRTEDGVLVDESPVSAPLDYLHGHGSLISG
                     LETALEGHEVGDKFDVAVGANDAYGQYDENLVQRVPKDVFMGVDELQVGMRFLAETDQ
                     GPVPVEITAVEDDHVVVDGNHMLAGQNLKFNVEVVAIREATEEELAHGHVHGAHDHHH
                     DHDHDGCCGGHGHDHGHEHGGEGCCGGKGNGGCGCH"
     gene            complement(7859..8059)
                     /gene="yheV"
                     /locus_tag="ACHOSC_RS06340"
                     /old_locus_tag="pgaptmp_001268"
     CDS             complement(7859..8059)
                     /gene="yheV"
                     /locus_tag="ACHOSC_RS06340"
                     /old_locus_tag="pgaptmp_001268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YheV family putative zinc ribbon protein"
                     /protein_id="HGU4547244.1"
                     /translation="MAIRKRFIAGAKCPACQAQDSMAMWRENNIDIVECVKCGHQMRE
                     ADKEARDHVRKDEQVIGIFHPD"
     gene            complement(8069..9874)
                     /gene="kefB"
                     /locus_tag="ACHOSC_RS06345"
                     /old_locus_tag="pgaptmp_001269"
     CDS             complement(8069..9874)
                     /gene="kefB"
                     /locus_tag="ACHOSC_RS06345"
                     /old_locus_tag="pgaptmp_001269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015503 - glutathione-regulated potassium
                     exporter activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-regulated potassium-efflux system
                     protein KefB"
                     /protein_id="HGU4547245.1"
                     /translation="MEGSDFLLAGVLFLFAAVAAVPLASRLGIGAVLGYLLAGIAIGP
                     WGLGFISDVDEILHFSELGVVFLMFIIGLELNPSKLWQLRRSIFGVGAAQVLLSAALL
                     AGLLMLTDFAWQAAVVGGIGLAMSSTAMALQLMREKGMNRSESGQLGFSVLLFQDLAV
                     IPALALVPLLAGSADEHFDWMKIGMKVLAFVGMLIGGRYLLRPVFRFIAASGVREVFT
                     AATLLLVLGSALFMDALGLSMALGTFIAGVLLAESEYRHELETAIDPFKGLLLGLFFI
                     SVGMSLNLGVLYTHLLWVVISVVVLVAVKILVLYLLARLYGVRSSERMQFAGVLSQGG
                     EFAFVLFSTASSQRLFQGDQMALLLVTVTLSMMTTPLLMKLVDKWLSRQFNGPEEEDE
                     KPWVNDDKPQVIVVGFGRFGQVIGRLLMANKMRITVLERDISAVNLMRKYGYKVYYGD
                     ATQVDLLRSAGAEAAESIVITCNEPEDTMKLVEICQQHFPHLHILARARGRVEAHELL
                     QAGVTQFSRETFSSALELGRKTLVTLGMHPHQAQRAQLHFRRLDMRMLRELIPMHADT
                     VQISRAREARRELEEIFQREMQQERRQLDGWDEFE"
     gene            complement(9874..10425)
                     /gene="kefG"
                     /locus_tag="ACHOSC_RS06350"
                     /old_locus_tag="pgaptmp_001270"
     CDS             complement(9874..10425)
                     /gene="kefG"
                     /locus_tag="ACHOSC_RS06350"
                     /old_locus_tag="pgaptmp_001270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312229.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-regulated potassium-efflux system
                     ancillary protein KefG"
                     /protein_id="HGU4547246.1"
                     /translation="MSQPAKVLLLFAHPESQDSVANRVLLKPATQLSNVTVHDLYAHY
                     PDFFIDIPREQALLREHEVIVFQHPLYTYSCPALLKEWLDRVLSRGFASGPGGNQLAG
                     KYWRSVITTGEPESAYRYDALNRYPMSDVLRPFELAAGMCRMHWLSPIIIYWARRQSA
                     QELASHARAYGDWLANPLSPGGR"
     gene            10556..12469
                     /gene="yheS"
                     /locus_tag="ACHOSC_RS06355"
                     /old_locus_tag="pgaptmp_001271"
     CDS             10556..12469
                     /gene="yheS"
                     /locus_tag="ACHOSC_RS06355"
                     /old_locus_tag="pgaptmp_001271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HGU4547247.1"
                     /translation="MIVFSSLQIRRGVRVLLDNATATINPGQKVGLVGKNGCGKSTLL
                     ALLKNEISADGGSYTFPGSWQLAWVNQETPALPQAALEYVIDGDREYRQLEAQLHDAN
                     ERNDGHAIATIHGKLDAIDAWSIRSRAASLLHGLGFSNEQLERPVSDFSGGWRMRLNL
                     AQALICRSDLLLLDEPTNHLDLDAVIWLEKWLKSYQGTLILISHDRDFLDPIVDKIIH
                     IEQQSMFEYTGNYSSFEVQRATRLAQQQAMYESQQERVAHLQSYIDRFRAKATKAKQA
                     QSRIKMLERMELIAPAHVDNPFRFSFRAPESLPNPLLKMEKVSAGYGDRIILDSIKLN
                     LVPGSRIGLLGRNGAGKSTLIKLLAGELAPVSGEIGLAKGIKLGYFAQHQLEYLRADE
                     SPIQHLARLAPQELEQKLRDYLGGFGFQGDKVTEETRRFSGGEKARLVLALIVWQRPN
                     LLLLDEPTNHLDLDMRQALTEALIEFEGALVVVSHDRHLLRSTTDDLYLVHDRKVEPF
                     DGDLEDYQQWLSDVQKQENQTDEAPKENANSAQARKDQKRREAELRAQTQPLRKEIAR
                     LEKEMEKLNAQLAQAEEKLGDSELYDQSRKAELTACLQQQASAKSGLEECEMAWLEAQ
                     EQLEQMLLEGQSN"
     gene            12469..13491
                     /gene="yheT"
                     /locus_tag="ACHOSC_RS06360"
                     /old_locus_tag="pgaptmp_001272"
     CDS             12469..13491
                     /gene="yheT"
                     /locus_tag="ACHOSC_RS06360"
                     /old_locus_tag="pgaptmp_001272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydrolase"
                     /protein_id="HGU4547248.1"
                     /translation="MAQITTTDANEFSSSAEFIPMRGFSNCHLQTMLPRLFRRQVKFT
                     PYWQRLELPDGDFVDLAWSENPAQAQHKPRLVVFHGLEGSLNSPYAHGLVEAAQKRGW
                     LGVVMHFRGCSGEPNRMHRIYHSGETEDASWFLRWLQREFGHAPTAAVGYSLGGNMLA
                     CLLAKEGNDLPVDAAVIVSAPFMLEACSYHMEKGFSRVYQRYLLNLLKANAARKLAAY
                     PGTLPINLAQLKSVRRIREFDDLITARIHGYADAIDYYRQCSAMPMLNRIAKPTLIIH
                     AKDDPFMDHQVIPKPESLPPQVEYQLTEHGGHVGFIGGTLLHPQMWLESRIPDWLTTY
                     LEAKSC"
     gene            13485..13703
                     /gene="yheU"
                     /locus_tag="ACHOSC_RS06365"
                     /old_locus_tag="pgaptmp_001273"
     CDS             13485..13703
                     /gene="yheU"
                     /locus_tag="ACHOSC_RS06365"
                     /old_locus_tag="pgaptmp_001273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YheU family protein"
                     /protein_id="HGU4547249.1"
                     /translation="MLIPWQDLSPETLENLIESFVLREGTDYGEHERTLEQKVADVKR
                     QLQCGEAVLVWSELHETVNIMPRSQFRE"
     gene            13757..14626
                     /gene="prkB"
                     /locus_tag="ACHOSC_RS06370"
                     /old_locus_tag="pgaptmp_001274"
     CDS             13757..14626
                     /gene="prkB"
                     /locus_tag="ACHOSC_RS06370"
                     /old_locus_tag="pgaptmp_001274"
                     /EC_number="2.7.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008974 - phosphoribulokinase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribulokinase"
                     /protein_id="HGU4547250.1"
                     /translation="MSAKHPVIAVTGSSGAGTTTTSLAFRKIFAQLNLHAAEVEGDSF
                     HRYTRPEMDMAIRKARDAGRHISYFGPEANDFGLLEQTFIEYGQSGKGKSRKYLHTYD
                     EAVPWNQVPGTFTPWQPLPEPTDVLFYEGLHGGVVTPQHNVAQHVDLLVGVVPIVNLE
                     WIQKLIRDTSERGHSREAVMDSVVRSMEDYINYITPQFSRTHLNFQRVPTVDTSNPFA
                     AKGIPSLDESFVVIHFRNLEGIDFPWLLAMLQGSFISHINTLVVPGGKMGLAMELIML
                     PLVQRLMEGKKIE"
     gene            complement(14681..15085)
                     /gene="yhfA"
                     /locus_tag="ACHOSC_RS06375"
                     /old_locus_tag="pgaptmp_001275"
     CDS             complement(14681..15085)
                     /gene="yhfA"
                     /locus_tag="ACHOSC_RS06375"
                     /old_locus_tag="pgaptmp_001275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OsmC family protein"
                     /protein_id="HGU4547251.1"
                     /translation="MQARVKWVEGLTFLGESASGHQILMDGNSGDKAPSPMEMVLMAA
                     GGCSAIDVVSILQKGRQDVVDCEVKLTSERREEAPRLFTHINLHFIVTGRDLKDAAVA
                     RAVDLSAEKYCSVALMLEKAVNITHSYEVVAA"
     gene            15387..16019
                     /gene="crp"
                     /locus_tag="ACHOSC_RS06380"
                     /old_locus_tag="pgaptmp_001276"
     CDS             15387..16019
                     /gene="crp"
                     /locus_tag="ACHOSC_RS06380"
                     /old_locus_tag="pgaptmp_001276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cAMP-activated global transcriptional regulator
                     CRP"
                     /protein_id="HGU4547252.1"
                     /translation="MVLGKPQTDPTLEWFLSHCHIHKYPSKSTLIHQGEKAETLYYIV
                     KGSVAVLIKDEEGKEMILSYLNQGDFIGELGLFEEGQERSAWVRAKTACEVAEISYKK
                     FRQLIQVNPDILMRLSAQMARRLQVTSEKVGNLAFLDVTGRIAQTLLNLAKQPDAMTH
                     PDGMQIKITRQEIGQIVGCSRETVGRILKMLEDQNLISAHGKTIVVYGTR"
     gene            16070..18160
                     /gene="yhfK"
                     /locus_tag="ACHOSC_RS06385"
                     /old_locus_tag="pgaptmp_001277"
     CDS             16070..18160
                     /gene="yhfK"
                     /locus_tag="ACHOSC_RS06385"
                     /old_locus_tag="pgaptmp_001277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312236.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YccS/YhfK family putative transporter"
                     /protein_id="HGU4547253.1"
                     /translation="MWRRLIYHPDINYALRQTLVLCLPVAVGLMLGELRFGLLFSLVP
                     ACCNIAGLDTPHKRFFKRLIIGASLFATCSLLTQLLLAKDVPLPFLLTGLTLVLGVTA
                     ELGPLHAKLLPASLLAAIFTLSLAGYMPVWEPLLIYALGTLWYGLFNWFWFWIWREQP
                     LRESLSLLYRELADYCEAKYSLLTQHTDPEKALPPLLVRQQKAVDLITQCYQQMHMLS
                     AQNNTDYKRMLRIFQEALDLQEHISVSLHQPEEVQKLVERSHAEEVIRWNAQTVAARL
                     RVLADDILYHRLPTRFTMEKQIGALEKIARQHPDNPVGQFCYWHFSRIARVLRTQKPL
                     YARDLLADKQRRMPLLPALKSYLSLKSPALRNAGRLSVMLSVASLMGTALHLPKSYWI
                     LMTVLLVTQNGYGATRLRIVNRSVGTVVGLIIAGVALHFKIPEGYTLTLMLITTLASY
                     LILRKNYGWATVGFTITAVYTLQLLWLNGEQYILPRLIDTIIGCLIAFGGTVWLWPQW
                     QSGLLRKNAHDALEAYQEAIRLILSEDPQPTPLAWQRMRVNQAHNTLYNSLNQAMQEP
                     AFNSHYLADMKLWVTHSQFIVEHINAMTTLAREHRALPPELAQEYLQSCEIAIQRCQQ
                     RLEYDEPGSSGDANIMDAPEMQPHEGAAGTLEQHLQRVIGHLNTMHTISSMAWRQRPH
                     HGIWLSRKLRDSKA"
     gene            complement(18227..19447)
                     /gene="argD"
                     /locus_tag="ACHOSC_RS06390"
                     /old_locus_tag="pgaptmp_001278"
                     /gene_synonym="dapC"
     CDS             complement(18227..19447)
                     /gene="argD"
                     /locus_tag="ACHOSC_RS06390"
                     /old_locus_tag="pgaptmp_001278"
                     /gene_synonym="dapC"
                     /EC_number="2.6.1.11"
                     /EC_number="2.6.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     acetylornithine/succinyldiaminopimelate transaminase"
                     /protein_id="HGU4547254.1"
                     /translation="MAIEQTAITRATFDEVILPIYAPAEFIPVKGQGSRIWDQQGKEY
                     VDFAGGIAVTALGHCHPALVNALKTQGETLWHISNVFTNEPALRLGRKLIEATFAERV
                     VFMNSGTEANETAFKLARHYACVRHSPFKTKIIAFHNAFHGRSLFTVSVGGQPKYSDG
                     FGPKPADIIHVPFNDLHAVKAVMDDHTCAVVVEPIQGEGGVTAATPEFLQGLRELCDQ
                     HQALLVFDEVQCGMGRTGDLFAYMHYGVTPDILTSAKALGGGFPISAMLTTAEIASAF
                     HPGSHGSTYGGNPLACAVAGAAFDIINTPEVLEGIQAKRQRFVDHLQKIDQQYDVFSD
                     IRGMGLLIGAELKPQYKGRARDFLYAGAEAGVMVLNAGPDVMRFAPSLVVEDADIDEG
                     MQRFAHAVAKVVGA"
     gene            complement(19533..20096)
                     /gene="pabA"
                     /locus_tag="ACHOSC_RS06395"
                     /old_locus_tag="pgaptmp_001279"
     CDS             complement(19533..20096)
                     /gene="pabA"
                     /locus_tag="ACHOSC_RS06395"
                     /old_locus_tag="pgaptmp_001279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminodeoxychorismate synthase component 2"
                     /protein_id="HGU4547255.1"
                     /translation="MILLIDNYDSFTWNLYQYFCELGADVLVKRNDALTLADIDALKP
                     QKIVISPGPCTPDEAGISLDVIRHYAGRLPILGVCLGHQAMAQAFGGKVVRAAKVMHG
                     KTSPITHNGEGVFRGLANPLTVTRYHSLVVEPGSLPACFDVTAWSETREIMGIRHRQW
                     DLEGVQFHPESILSEQGHQLLANFLHR"
     gene            complement(20128..20730)
                     /gene="fic"
                     /locus_tag="ACHOSC_RS06400"
                     /old_locus_tag="pgaptmp_001280"
     CDS             complement(20128..20730)
                     /gene="fic"
                     /locus_tag="ACHOSC_RS06400"
                     /old_locus_tag="pgaptmp_001280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative adenosine monophosphate-protein
                     transferase Fic"
                     /protein_id="HGU4547256.1"
                     /translation="MSDKFGEGRDPYLYPGLDIMRNRLNIHQQQRLEQAAYEMTALRA
                     ATIELGPLVRGLPHLRTIHRQLYQDIFDWAGQLREVDIYQGDTPFCHFAYIEKEGNAL
                     MQDLEEEGYLVGLEKAKFVERLAHYYCEINVLHPFRVGSGLAQRIFFEQLAIHAGYQL
                     SWQGIEKEAWNQANQSGAMGDLTALQMIFSKVVSEAGESE"
     gene            complement(20720..20887)
                     /gene="yhfG"
                     /locus_tag="ACHOSC_RS06405"
                     /old_locus_tag="pgaptmp_001281"
     CDS             complement(20720..20887)
                     /gene="yhfG"
                     /locus_tag="ACHOSC_RS06405"
                     /old_locus_tag="pgaptmp_001281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhfG family protein"
                     /protein_id="HGU4547257.1"
                     /translation="MKKLTDKQKSRLWELQRNRNFQASRRLEGVEMPLVTLTAAEALA
                     RLEELRRHYER"
     gene            complement(20992..21564)
                     /gene="ppiA"
                     /locus_tag="ACHOSC_RS06410"
                     /old_locus_tag="pgaptmp_001282"
                     /gene_synonym="rotA"
     CDS             complement(20992..21564)
                     /gene="ppiA"
                     /locus_tag="ACHOSC_RS06410"
                     /old_locus_tag="pgaptmp_001282"
                     /gene_synonym="rotA"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase A"
                     /protein_id="HGU4547258.1"
                     /translation="MFKSTLAAMAAVFALSALSPAAMAAKGDPHVLLTTSAGNIELEL
                     DKQKAPVSVQNFVDYVNSGFYNNTTFHRVIPGFMIQGGGFTEQMQQKKPNPPIKNEAD
                     NGLRNTRGTIAMARTADKDSATSQFFINVADNAFLDHGQRDFGYAVFGKVVKGMDVAD
                     KISQVPTHDVGPYQNVPSKPVVILSAKVLP"
     gene            21835..23016
                     /gene="tsgA"
                     /locus_tag="ACHOSC_RS06415"
                     /old_locus_tag="pgaptmp_001283"
     CDS             21835..23016
                     /gene="tsgA"
                     /locus_tag="ACHOSC_RS06415"
                     /old_locus_tag="pgaptmp_001283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter TsgA"
                     /protein_id="HGU4547259.1"
                     /translation="MTNSNRIKLTWISFLSYALTGALVIVTGMVMGNIADYFNLPVSS
                     MSNTFTFLNAGILISIFLNAWLMEIVPLKTQLRFGFLLMVLAVAGLMFSHSLALFSAA
                     MFILGVVSGITMSIGTFLVTQMYEGRQRGSRLLFTDSFFSMAGMIFPMIAAFLLARSI
                     EWYWVYACIGLVYVAIFILTFGCEFPALGKHAPKTDAPVEKEKWGIGVLFLSVAALCY
                     ILGQLGFISWVPEYAKGLGMSLNDAGTLVSNFWMSYMVGMWAFSFILRFFDLQRILTV
                     LAGLAAILMYVFNTGTPAHMAWSILALGFFSSAIYTTIITLGSQQTKVPSPKLVNFVL
                     TCGTIGTMLTFVVTGPIVEHSGPQAALLTANGLYAVVFVMCFLLGFVSRHRQHNTLTS
                     H"
     gene            23278..25821
                     /gene="nirB"
                     /locus_tag="ACHOSC_RS06420"
                     /old_locus_tag="pgaptmp_001284"
     CDS             23278..25821
                     /gene="nirB"
                     /locus_tag="ACHOSC_RS06420"
                     /old_locus_tag="pgaptmp_001284"
                     /EC_number="1.7.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709140.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-nitrite reductase large subunit"
                     /protein_id="HGU4547260.1"
                     /translation="MSKVRLAIIGNGMVGHRFIEDLLDKSDAANFDITVFCEEPRIAY
                     DRVHLSSYFSHHTAEELSLVREGFYEKHGIKVLVGERAITINRQEKVIHSSAGRTVFY
                     DKLIMATGSYPWIPPIKGSDTQDCFVYRTIEDLNAIESCARRSKRGAVVGGGLLGLEA
                     AGALKNLGIETHVIEFAPMLMAEQLDQMGGEQLRRKIESMGVRVHTSKNTLEIVQEGV
                     EARKTMRFADGSELEVDFIVFSTGIRPRDKLATQCGLDVAPRGGIVINDSCQTSDPDI
                     YAIGECASWNNRVFGLVAPGYKMAQVAVDHILGSENAFEGADLSAKLKLLGVDVGGIG
                     DAHGRTPGARSYVYLDESKEIYKRLIVSEDNKTLLGAVLVGDTSDYGNLLQLVLNAIE
                     LPENPDSLILPAHSGSGKPSIGVDKLPDSAQICSCFDVTKGDLIAAINKGCHTVAALK
                     AETKAGTGCGGCIPLVTQVLNAELAKQGIEVNNNLCEHFAYSRQELFHLIRVEGIKTF
                     EELLAKHGKGYGCEVCKPTVGSLLASCWNEYILKPEHTPLQDSNDNFLANIQKDGTYS
                     VIPRSPGGEITPEGLMAVGRIAREFNLYTKITGSQRLAMFGAQKDDLPEIWRQLIEAG
                     FETGHAYAKALRMAKTCVGSTWCRYGVGDSVGLGVELENRYKGIRTPHKMKFGVSGCT
                     RECSEAQGKDVGIIATEKGWNLYVCGNGGMKPRHADLLAADIDRETLIKYLDRFMMFY
                     IRTADKLTRTAPWLENLEGGIDYLKAVIIDDKLGLNAHLEEEMARLREAVVCEWTETV
                     NTPSAQTRFKHFINSDKRDPNVQMVPEREQHRPATPYERIPVTLVEDNA"
     gene            25818..26144
                     /gene="nirD"
                     /locus_tag="ACHOSC_RS06425"
                     /old_locus_tag="pgaptmp_001285"
     CDS             25818..26144
                     /gene="nirD"
                     /locus_tag="ACHOSC_RS06425"
                     /old_locus_tag="pgaptmp_001285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrite reductase small subunit NirD"
                     /protein_id="HGU4547261.1"
                     /translation="MSQWKDICKIDDILPETGVCALLGDEQVAIFRPYHSDQVFAISN
                     IDPFFESSVLSRGLIAEHQGELWVASPLKKQRFRLSDGLCMEDEQFSVKHYEARVKDG
                     VVQLRG"
BASE COUNT         6373 a         7111 c         6582 g         6168 t
ORIGIN      
        1 taagaatcca ggaagccagc cagttccagg attttcgctt cccactctgc gtcgccttcc
       61 agcgctttca gagcagaacc acgaacgatc ggagtgtcgt cgcccgggaa gtcgtactga
      121 gacagaagtt cacgaacttc catttcaacc agttccagca gctcttcgtc atcaaccatg
      181 tcgcatttgt tcaggaacac gatgatgtac ggaacgccta cctgacgacc cagcaggatg
      241 tgctcacgag tctgcggcat cgggccgtca gtcgcagcaa ctaccaggat cgcgccgtcc
      301 atctgagcag caccggtgat catgttttta acatagtcgg cgtgccccgg gcagtctacg
      361 tgtgcgtagt gacgggtcgg ggtgtcatat tcaacgtgag aagtgttgat ggtgatacca
      421 cgagcttttt cttccggcgc gttatcgatc tggtcgaatg cacgagcagc accgccgtag
      481 gttttagcca gtacggtggt gattgcagcg gtcagagtag ttttaccgtg gtcaacgtgg
      541 ccgatagtac caacgttaac gtgcggtttt gtacgttcaa atttttcttt agacacggct
      601 atattcctta ctatagtgct ctccccttca ggagagagca cgggactttg gtattaaccc
      661 ttaggcttat ttaccacggg cttcaattac ggcctgagca acgttactcg gcgcttcatc
      721 atacttcagg aattccatag tgtatgatgc acgacctttg gtcagagaac gcagctgagt
      781 tgcgtatccg aacatttcag acagcggtac ttcagcgtgg atcttaacgc cagtaacttc
      841 agattcctga cctttgagca taccacgacg acggctcaag tcaccgataa cgtcaccggt
      901 gttctcttcc ggagtttcta cttcaacctt catgatcggc tcaagcagaa ctggtttcgc
      961 tttcttaaag ccttctttaa aggcgataga agcagccagt ttaaacgcca gttcagagga
     1021 gtcaacgtca tggtaagaac cgaagtgcag acgaataccc atgtctacta ccgggtagcc
     1081 tgccagcgga cctgctttca gctgttcctg gataccttta tcaacggccg ggatgtattc
     1141 gccagggatt acaccacctt taatgtcgtt gatgaactcg tagcctttcg ggtttgaacc
     1201 cggctccagc gggtacatgt cgataacaac atgaccatac tgaccacgac caccagactg
     1261 tttcgcgtgt ttaccttcaa catcggtaac tttctggcgg atagtttcac ggtaagcaac
     1321 ctgcggttta cctacgttcg cttcaacgtt gaattcacgc ttcatacggt caacgatgat
     1381 gtcgaggtgc agttcgccca tacccgcgat aatggtctgg ttagattctt cgtcagtcca
     1441 tacacggaaa gacgggtctt ctttagccag acggcccaga gccagaccca ttttttcctg
     1501 gtcagctttg gttttcggtt caactgcgat ggagattacc ggctcaggga attccatacg
     1561 ttccagaatg atcggcgcat ccgggtcaca cagggtgtca ccagtggtta cgtctttcag
     1621 accgatagca gcagcgatgt cgcccgcgcg aacttctttg atctcttcac gtttgttagc
     1681 gtgcatctga acgatacgac cgaaacgctc acgtgcagct ttcacggagt tcagtacggt
     1741 atcaccagag ttaaccacac cggagtaaac acggaagaag gtcaggttac caacaaacgg
     1801 gtcggtagcg attttgaacg ccagtgcaga aaacggctcg tcatcacttg cgtgacgctc
     1861 agccggagtg tctttaccgt cgtccaggat accgttgatc gcaggtacgt caaccgggga
     1921 tggcaggtaa tcaattaccg catccagcat cgcctgaaca cctttgttct tgaacgcaga
     1981 accacaggtt accaggatga tttcgttgtt cagaacgcgc tgacgcagag cacctttgat
     2041 ttctgcttca gtcagttctt caccacccag gtatttttcc atcagctctt cagaagcttc
     2101 agctgcggat tcgatcaggt tctggtgcca ttcgttagcc agttcaacca tgtctgccgg
     2161 gatatcttcg tattcgaagg ttacgccctg gtcagcgtcg ttccagttga tagctttcat
     2221 tttcaccagg tcaacaacac cggtgaaatg ttcttcagca ccaatcgcca gctgcagcgg
     2281 aaccgggttc gcgcccagac gggttttgat ctggttaaca actttcagga agttcgcacc
     2341 catgcggtcc attttgttaa cgaacgcaat gcgcggaact ttatatttgt ttgcctgacg
     2401 ccatacggtt tcagactgcg gctgaacacc accaactgcg cagtaaacca ttaccgcacc
     2461 atcgagaaca cgcatggaac gttctacttc gattgtgaag tcaacgtgcc ccggggtgtc
     2521 gatgatgttg atgcgatgcg gctcatactg cttagccata ccagaccaga atgcagtagt
     2581 cgcagcggaa gtgatggtaa taccacgttc ctgctcctgc tccatccagt ccatggttgc
     2641 agcgccgtca tgaacttcac cgattttatg gtttacaccg gtgtagaaca gaatacgttc
     2701 ggtagtagtg gttttaccgg cgtcgatgtg cgcactgata ccgatattac ggtagcgtgc
     2761 gatgggtgtt gtacgagcca tttgtttcct cgtttatctt ttaggcgttc aatttaagta
     2821 gcccaaagcg ggctgcttac tggaagcgcc cgcctggtga ctaaaactcc gaagggatta
     2881 ccaacggtag tgtgcgaacg ccttgttggc ttcggccata cggtgaacgt cttcacgttt
     2941 cttaactgca gtacctttgt tttctgcagc atcagaaagt tcgttcgcca ggcgcagagc
     3001 catggattta tcaccgcgtt tacgagcagc ttcaacgatc caacgcattg ccagagcatt
     3061 acgacgaacc ggacggactt caactggtac ctgataagta gaaccaccaa cgcggcgaga
     3121 cttaacttct acagtcgggc gcacgttttc gagagctact tcgaatgctt ccagttcaga
     3181 tttaccagag cgctgagcca gggtctccag cgcgctgtat acgatagatt cagcagtaga
     3241 ttttttacca tctaccatca ggatatttac aaatttagcc agcagttctg atccgaactt
     3301 cggatccggc agaattttac gctgaccaat gacgcgacga cgtggcatgg aaatactccg
     3361 ttgttaattc aggattgtcc aaaactctac gagtttagtt tgacatttaa gttaaaacgt
     3421 ttggccttac ttaacggaga accattaagc cttaggacgc ttcacgccat acttggaacg
     3481 agcctgctta cggtctttaa cgccggagca gtcaagcgca ccacgtacgg tgtggtaacg
     3541 aacacccggg aggtctttaa cacgaccgcc acggatcagg atcacggagt gctcctgcag
     3601 gttgtgacct tcaccaccga tgtaggaagt cacttcgaaa ccgttagtca gacgaacacg
     3661 gcatacttta cgcagcgcgg agttcggttt tttaggagtg gtagtatata cacgagtaca
     3721 tacgccacgt ttttgcgggc atgcttccag cgcaggcacg ttgcttttcg caactttgcg
     3781 agcacgtggt ttgcgtacca gctggttaac tgttgccatt aaatagctcc tggttttagc
     3841 ttttgcttcg taaacacgta ataaaacgtc ctcacacaat atgaggacgc cgaattttag
     3901 ggcgatgccg aaaaggtgtc aagaaatata caacgatccc gccatcacca ggccatctgg
     3961 ctggagtgct taaccgtaag tctgacgaaa tcagtatagt caatgggaat gatgtcgttc
     4021 gaaatttgac cagtcaaacc gcgggcaata aggtcttcgt tcagggcata gaccttaatg
     4081 ggggcattac gcagactttc aaggtagcgg ttaccgtcaa ctgcggcagt tacgccatct
     4141 tgcaataata gcagttcgtc tccttcactg agcagacgca gcagcgcagc aaaatccgtc
     4201 agccagggtg aacgatgtaa cgtgtgcagc ataaacgcct caaaacctca aaataacatc
     4261 gtagttggcg agttcgcggc gtaaggcatc tgcttcgagc ggcgtggctt cgacaacaaa
     4321 gggtgtctgc ggatctaacc cgcgttcgcg cagtgaagcc gcacaaaccc agcactgttc
     4381 aatgtcgtac agacccaaca atttaaaagt ggcaatgtaa tcacgcgcca gcactgcatc
     4441 gggcttttgt cctgacagca gctgaaaaac gccatcagca ataaagaaga cggccagatc
     4501 gtcagttaat gcggaagttg ccagtaaagc atctaaacct tcccgaccag cagctgtacc
     4561 atgaggtgca gtagaaaaaa caaacgcaat tcgtttcatc agaactgtac caccctgtca
     4621 caggtcagcg aggcttccgc cagcgcgcca agtccgctta aggtaaatcc cggctgaagg
     4681 tttgacgaag ccagccccag ccttcctgct tcagtttcat cgacgatacc gcggcgtaac
     4741 gctgccgcta cgcagatatt cagcgccaca ccatgttgcg cattcaactg ttgccagccg
     4801 cgcacaaggt caaattcgtc acttgccggt gaggtcaatt ggttagcgtt atagacccct
     4861 tcccgataga aaaagacgct gcttaactca tggccttcta ctatcagcgc ctgcgcaaac
     4921 tgaaaagcac tactcgcctg ttgcgtaccg tatgctggcc cggtcaccac gatggcaaaa
     4981 cgcattactt atcttgcccc tggaaatcac cgcttttgaa ctggcggatg tagagataga
     5041 cagtgtgttt ggagatgttc aggcggtcag caacctggtt gatcgcgtct ttaatatcga
     5101 agatcccttt ctcgtagaga ttcagcacga tctgacgatt tttggcgtta ttagaaacat
     5161 tgcgatcggc attcacttct tcgatggtga actccagcgt ttgggtaacc agatcttcaa
     5221 cagaagaggc aaagttgacg cttgaaccga catccggggt ttctggcggc acaaaagtgc
     5281 tcataatctg cgagaaggga acatcaagat tcatattgat gcacagcaga ccaattacac
     5341 gctgttcgcg gttacgaatc gcgatagtca gggacttcat taatacgccg cttttggcgc
     5401 gagtaaagta gcatttagaa acgctgctat ccgctcccgt catatcgtgc agcatacgta
     5461 gcgccaggtc agtaattggc gaaccaatct tccggcctgt atgttcaccg ttagcaatgc
     5521 gaatggctga acattttaga tcctgcaaag agtgcaaaac gatttcacag tgggagccaa
     5581 taagcatcgc taacccgtcc accaccgctt cgtaggattt cagaatatca aaatcggtct
     5641 ggtcgaaagg acgttgatcc agtaaatcca actcactggt ttcgttggtt aaaagcgacc
     5701 tggacatgaa aaaaaacact ccttttcagg agcctgtcgt taacttttca gggcaggctc
     5761 attaatgatg cgggtaacta aattaataca gcggaggttc cgctttccag cactaattat
     5821 atccggcctg taataaaaaa accgccgcct ggtcaggcgg cggttcttaa tgcttatttt
     5881 ttagcagaat ctgcggcttt cgcatcagct tccggctttg catcagcctt cggcgctggt
     5941 ttcacatcca gcagctctac gtcaaacacc agggtagaat tcggtgggat ccccggaaca
     6001 cccgctttgc cgtaagccag ttctggtgga ataaccagtt tgatcttacc gcctttcttg
     6061 atgttcttca gaccttctgt ccaacccggg ataacaccgt ccagacggaa agaaagcggt
     6121 tcaccacggg tgtaagagtt gtcgaactct ttaccgtcga tcagcgtacc tttgtagttc
     6181 actacaacag tatcgctgtc tttcggtgct tcgcctttac cggcttctac tacctgataa
     6241 accagaccag ttgaagaggt tttcacacct ttctctttgg caaatttctc gcggtactct
     6301 ttaccttttg cttcgttatc agccgcgtct ttttccatct tcgcctgagc agaagacttc
     6361 acgcgagctt cgaatgcttg cagagtctgt tcgatctctt ggtcggagag tttgctctta
     6421 tcagcaaatg catcctgaac accagcgatc agctgatctt tatccagttt gatgcccagt
     6481 ttttcttgtt ctttaagaga gttttccatg taacgaccca gcgaagcacc cagtgcataa
     6541 gctgatttct gatcgtcatt tttgaacgct gctttgctgt cagcagttgt agcaggtttt
     6601 gcagcttcag cagcaaaagt gattggtgca tgcagggcaa cggccattgt ggtcgccagc
     6661 agcgttactt taaacagtga tttcatccat atctccagga tcggggcatc tcaccccagg
     6721 gttaactatt atcagaaggg tactataaag cgttgtcgaa caaatctaca tacagacacg
     6781 ccctattatc atctattttc agactctttt tgtttaaatt agtttcgatg accgcgaaat
     6841 gagtgctgtc tcgggcagca aagttaagta gaatccgcgg cgaccattcg acaaaagagg
     6901 tgaatcatgc aggatttatc attggaagca cgcctggcag agctggagag ccgactggct
     6961 tttcaggaaa tcaccattga agaactgaac gtcacggtga ccgctcatga aatggagatg
     7021 gcgaaactgc gcgatcatct gcgtctgctg accgagaagt taaaagccag ccagccgtcg
     7081 aacatcgcgt cgcaggctga agaaacgcca ccgccacatt attgaggcgt aaaaaaagcg
     7141 gggattcccc gcttttttgt caccttttcg gtattagtgg caaccacaac cgccgttgcc
     7201 tttaccgcca cagcagcctt cgccaccgtg ttcatgaccg tgatcgtggc catgaccgcc
     7261 gcagcaaccg tcgtggtcgt gatcgtggtg atgatcgtgc gcgccgtgaa cgtggccatg
     7321 agccagttct tcttcggtcg cttcgcgaat cgccacaact tcaacgttga atttcaggtt
     7381 ctgaccggcc agcatgtggt taccatcaac cacgacgtga tcgtcttcaa ccgcagtgat
     7441 ttcaaccggt accggcccct ggtcggtttc agccaggaaa cgcataccta cctgcagttc
     7501 atcaacgccc ataaatacgt ctttaggaac acgttgcacc aggttttcgt cgtactgacc
     7561 gtaagcgtcg ttcgcgccaa cagcgacatc aaatttgtcg ccaacttcat gaccttccag
     7621 cgccgtttcc aggccagaga tcagggaacc gtgaccatgc aggtagtcca gcggcgcact
     7681 caccggagac tcatcaacca acacaccgtc ttctgtacgt acctgatagg ccaggctgac
     7741 caccaggtct tttgctactt tcatgatatc tcctgagcat gggaagaata gtggcgcaga
     7801 ttgtagcgga attctgcagc cgtgtactca ctagcttaaa aaaactcggc gcatatcgct
     7861 agtccggatg aaaaatcccg atcacttgct catctttgcg aacgtgatcg cgggcttctt
     7921 tgtctgcttc tcgcatctga tgtccgcact taacacattc aacaatatca atattatttt
     7981 cgcgccacat cgccattgaa tcctgcgcct gacaggccgg gcattttgcg cccgcgataa
     8041 aacgttttcg gattgccatc tttaccctct actcaaattc atcccagccg tccagctggc
     8101 gtcgttcttg ttgcatttca cgctggaaaa tctcttccag ttcgcgtcgg gcttccctgg
     8161 cgcgagaaat ttgtacggta tcagcatgca tcgggattaa ctcccgcagc attcgcatat
     8221 ccaggcggcg aaaatgcagt tgcgcccgct gtgcctgatg cggatgcatg ccaagcgtga
     8281 ccagcgtctt gcgccccagc tctaacgcac tggagaatgt ttcacgggaa aactgcgtca
     8341 cccctgcctg taataactca tgcgcttcca cacgtccgcg cgctcgcgca agaatatgca
     8401 aatgcggaaa gtgctgctgg catatttcca ccagcttcat ggtgtcttcc ggctcgttac
     8461 aggtaatgac gatagactca gcggcctctg cacccgcaga acgtaaaaga tcgacctgcg
     8521 tggcgtcgcc gtaataaact ttgtagccat atttacgcat caggttaacg gcgctgatat
     8581 cccgctccag cacggtaatg cgcattttat ttgccatcag caaacgacca atcacctgac
     8641 caaaacgccc gaagcccacg acaatgacct ggggtttatc atcgttgacc cacggttttt
     8701 cgtcttcttc ttccggtccg ttaaactggc gggatagcca tttatccacc agcttcatca
     8761 gcaacggcgt ggtcatcatg gaaagcgtca ccgtcaccag caacagcgcc atctggtcgc
     8821 cctggaataa acgttgtgaa gaagcggtag aaaagaggac aaaggcaaac tcaccgccct
     8881 gactcaacac gccagcaaac tgcatccgct ctgagctacg cacgccatac aatcgcgcca
     8941 gcagatacag cacgagaatt ttcaccgcca ccagcacaac cacgcttatc actacccaca
     9001 acagatgggt ataaagcacc ccgaggttga gcgacatgcc gacagagata aagaacaaac
     9061 cgagcagcaa tcctttgaag ggatcgatag ccgtttccag ttcatggcga tattcacttt
     9121 ccgccagcag cacgcccgca ataaatgtac cgagcgccat cgacagcccc agcgcatcca
     9181 taaacaatgc ggaacccaac accagcagca gcgtcgcggc ggtgaacact tcccgcacgc
     9241 cagaagctgc aataaagcgg aataccggac gcagtaaata gcgcccacca atcagcatgc
     9301 cgacaaacgc cagcaccttc atgccgatct tcatccagtc gaaatgttcg tctgccgaac
     9361 ccgccaacaa cggcactaac gccagtgccg ggatcaccgc cagatcctga aacagcagta
     9421 ccgaaaaccc gagctgcccg gattcgctgc gattcatccc tttctcacgc atcaattgca
     9481 acgccattgc ggtcgaagac atcgcaaggc caatgccacc gaccaccgcc gcctgccagg
     9541 cgaaatccgt cagcatcaat aatcccgcca gcaacgccgc gcttaacagc acctgtgccg
     9601 cgcctacgcc aaaaatcgaa cgccgcagtt gccaaagctt ggagggattc aactcaaggc
     9661 cgatgataaa catcaggaat accacgccga gttccgaaaa gtggaggatc tcatcgacgt
     9721 cgctaataaa ccccagcccc cacgggccaa ttgcaatccc tgccagcaaa tatcccaaca
     9781 cagcgccaat ccccagccgc gatgccaacg gcaccgcagc caccgccgcg aagagaaaca
     9841 gcactcctgc gagtaaaaaa tcggaacctt ccatcagcgg cctcctggag acagcggatt
     9901 tgccagccag tcaccgtagg ctctggcgtg gctcgccagc tcctgtgcgc tttgccgtct
     9961 cgcccagtaa ataatgatgg gacttaacca atgcatccgg cacatgcccg ccgccagttc
    10021 aaagggacgc agaacgtcgc tcatggggta gcgattcaat gcgtcataac ggtaagcact
    10081 ttccggctcg ccggtggtga tcacgctacg ccagtacttt cccgccagtt ggtttcctcc
    10141 cggcccgctg gcaaaaccac ggcttaatac ccggtccagc cactctttca gtagcgccgg
    10201 gcagctatag gtataaagag gatgctgaaa gacaatcacc tcgtgctcgc gcagtaatgc
    10261 ctgctcacgg gggatatcaa taaaaaaatc gggatagtgc gcataaaggt cgtgcacggt
    10321 aacattgctg agctgcgtgg ccggtttaag cagtacccgg tttgccaccg agtcctgaga
    10381 ttccggatgg gcaaacagca gcaaaacttt cgctggctga gacatcatcc ccctcccggt
    10441 atggtttttg tgtatagtcg ctgttttggg ctaccattgc gcccggtgcg gcagctcgcc
    10501 catacattac attatcataa tgataagtta acatagtctg aacatacggc accttatgat
    10561 tgttttctcc tcgttacaaa ttcgtcgcgg cgtgcgcgtc ctgctggata atgccaccgc
    10621 caccatcaac cctgggcaga aagtcggcct ggtgggtaaa aacggctgtg gtaaatctac
    10681 cctgctggca ttgctgaaaa atgaaatcag cgccgacggc ggcagctaca cctttccggg
    10741 aagctggcaa ctggcgtggg tgaatcagga aacgccggcg ttaccgcaag cggcgctgga
    10801 atatgtcatt gacggcgacc gtgaatatcg tcaactagaa gcgcagctac acgacgccaa
    10861 cgaacgtaac gacgggcacg ccattgcgac cattcatggc aagctggatg ctattgacgc
    10921 atggagtatt cgctcccgtg ccgccagcct gctgcacggc ctcggtttca gcaatgaaca
    10981 actggagcgc ccggtaagtg atttctccgg tggctggcgt atgcgtctta accttgccca
    11041 ggcgctgatt tgccgttcag acttgctgct gctcgacgaa ccgactaacc acctcgatct
    11101 cgatgccgtt atctggctgg aaaaatggct gaagagctat cagggcacgc tgatcctgat
    11161 ctctcacgac cgcgacttcc tcgatccgat cgtcgataaa attattcata tcgaacaaca
    11221 aagcatgttc gagtacaccg gcaactacag ttcgtttgaa gtacagcgcg ccacccgtct
    11281 ggcgcagcaa caagcgatgt acgaaagcca gcaggaacgc gtagcgcatc tgcaaagtta
    11341 tatcgaccgt ttccgtgcca aagccaccaa agcgaagcag gcccagagcc gcattaagat
    11401 gctcgagcgt atggagctaa ttgcccccgc gcacgtcgac aacccgttcc gctttagctt
    11461 ccgcgcgccg gaaagcctgc caaatccgtt actgaagatg gaaaaagtca gcgcgggcta
    11521 tggcgatcgc attattctcg actcgattaa actgaacctg gtgcccggct cgcgcattgg
    11581 tctgttaggc cgcaatggcg cgggtaaatc gacattaatc aaactgttag ccggtgaact
    11641 tgcgccagtc agcggtgaaa ttggtctggc gaaagggatc aaactcggct acttcgccca
    11701 gcatcaactt gaatacctgc gcgccgacga atcacctatt caacatctgg cacgtttagc
    11761 gccgcaggag ctggaacaaa aactgcgtga ctacctcggc ggctttggtt tccagggcga
    11821 taaagtaacc gaagaaacgc gccgcttctc cggtggggaa aaagcccgcc tggtgctggc
    11881 attaattgtc tggcagcggc cgaatctgct gctgctcgac gaaccgacta accaccttga
    11941 cctcgacatg cgtcaggcac tcaccgaagc attaatcgag tttgaaggcg cgctggttgt
    12001 cgtttcgcac gaccgtcatt tgctgcgttc caccactgac gatctctacc tggttcacga
    12061 tcgtaaagtc gaaccgttcg acggcgatct ggaagattat caacagtggt tgagcgacgt
    12121 acaaaagcag gaaaaccaga ccgacgaagc gccaaaagag aatgcgaaca gcgcccaggc
    12181 acgtaaagat cagaagcgtc gggaagctga gctgcgtgcg caaacccagc cactgcgtaa
    12241 agagattgcc cgtctggaaa aagagatgga gaagctgaac gcgcaactgg cgcaggcgga
    12301 agagaaactc ggcgacagcg aactgtatga ccagagccgt aaagcggagt tgaccgcctg
    12361 cctgcaacag caagccagcg ccaaatccgg cctggaagag tgcgaaatgg catggctgga
    12421 agcccaggag cagcttgagc agatgctgct ggaaggccaa agcaactgat ggcgcagata
    12481 acgacgaccg atgccaatga attcagcagc agtgctgaat tcatccccat gcgcggcttt
    12541 agcaattgtc atctgcaaac catgctgccg cgtctgtttc gtcgtcaggt gaaattcacc
    12601 ccgtactggc agcggctgga gttgcccgac ggcgattttg tcgatcttgc gtggagtgaa
    12661 aaccctgcac aggcgcaaca taaacctcgt ctggtggtgt ttcacgggct ggaaggcagt
    12721 ctcaacagcc cttacgccca cggtctggtt gaggcggcgc aaaagcgcgg ctggctgggc
    12781 gtggtgatgc attttcgcgg atgcagcggt gaaccaaacc gtatgcaccg catttaccat
    12841 tcgggcgaaa ccgaagacgc cagttggttt ttacgctggc tgcaacgcga atttggtcat
    12901 gcgccaacgg ctgccgtcgg ctattcgctc ggcggtaata tgctggcctg tttgctggca
    12961 aaggaaggca atgatctccc ggttgatgcg gcggtgattg tctctgcgcc gtttatgctg
    13021 gaagcctgta gctatcatat ggaaaagggc ttttcccgcg tttatcagcg ttacttgctg
    13081 aacctgttaa aagccaatgc cgcgcgcaag ctggcagcct accccggaac gctgccgatt
    13141 aatctcgcgc agttaaaatc ggtacgtcgc atccgtgaat ttgacgatct catcaccgcc
    13201 agaattcacg gctacgccga cgctatcgac tattatcgtc agtgtagcgc catgccgatg
    13261 ctgaaccgga tcgccaaacc gacgctgatt attcacgcca aagacgatcc gtttatggat
    13321 catcaggtga tcccgaaacc ggaaagtctc cccccgcagg tggagtatca actgactgaa
    13381 catggcggtc atgttggctt tattggcggt acattacttc atccgcaaat gtggctggag
    13441 tcacgcattc ctgactggtt aacaacgtat ctggaggcga aatcatgttg attccgtggc
    13501 aagacctctc ccccgaaacg ctggaaaatt taattgaaag ctttgtgtta cgtgaaggca
    13561 ccgattatgg tgaacatgag cgtacacttg aacagaaagt cgccgacgtc aaacgccagc
    13621 tacagtgcgg agaagcggtg ctggtatggt cggagctgca cgaaacggtc aatattatgc
    13681 cgcgcagtca gtttcgcgaa taacctgcaa ccgtcggcgg acgagataat aatgatagct
    13741 aagtcatgga gttactatgt ctgccaaaca tccggtcatt gcggtaacag gatccagcgg
    13801 cgcggggacc accaccacca gcctcgcgtt tcgtaaaata ttcgcgcagt taaatctgca
    13861 tgcagctgag gtggaaggcg acagttttca ccgttacacc cgcccggaaa tggacatggc
    13921 gatccgcaaa gcgcgcgacg ccgggcggca tatcagctac ttcggccccg aggctaacga
    13981 cttcggcctg ctggaacaaa ccttcattga atacggtcag agcggcaaag ggaaatctcg
    14041 caaatatctg catacctacg acgaagccgt accgtggaat caggtaccgg ggacattcac
    14101 cccctggcaa cctttaccgg aacccactga tgtactgttt tatgaaggtt tacacggcgg
    14161 cgtagtcacg ccacagcata acgttgcgca gcatgtggac ttactggtcg gcgtggtgcc
    14221 tatcgttaac cttgagtgga ttcaaaaact gatccgcgac accagcgagc gcgggcactc
    14281 acgagaagca gtgatggact cagtagtgcg ttcaatggaa gactatatca actacatcac
    14341 accgcagttt tcccgcaccc atcttaactt ccagcgcgtt cccaccgtcg acacttcaaa
    14401 cccgttcgcg gcaaaaggta tcccgtcgct cgatgaaagc tttgtggtga tccattttcg
    14461 taatctggaa gggatcgatt tcccctggct gctggcgatg ttgcaaggct cattcatttc
    14521 ccacatcaat acgttagtgg taccgggcgg caaaatgggt ctggcaatgg aattaattat
    14581 gctgccgctg gtgcaacgat tgatggaagg aaagaaaatc gagtaactct gctattacgc
    14641 cggataaaat actatccggc ttcacaacgg gatagttaag tcacgcggca accacttcat
    14701 acgagtgagt aatattcacc gctttttcca gcatcaacgc cactgaacaa tatttctcgg
    14761 cagagagatc aaccgcacgc gcaaccgctg cgtctttcag gtcgcgaccg gtgacgataa
    14821 aatgcagatt aatgtgcgta aacaggcgtg gtgcctcttc gcggcgttca gaggtcaatt
    14881 ttacttcaca atcgaccaca tcctgacgcc ctttttgcag gatcgaaacc acatcgatgg
    14941 cactgcaacc acccgccgcc atcaacacca tttccatcgg acttggtgct ttatcgcctg
    15001 agttgccgtc cattaaaatc tgatgaccag aggcggattc gcccagaaaa gttaaccctt
    15061 cgacccactt cactcgcgct tgcatttttg ctactccact gcgtcaattt tcctgacaga
    15121 gtacgcgtac taaccaaatc gcgcaacgga aggcgacctg ggtcatgctg aagcgagaca
    15181 ccaggagaca caaagcgaaa gctatgctaa aacagtcagg atgctacagt aatacattga
    15241 tgtactgcat gtatgcaaag gacgtcacat taccgtgcag tacagttgat agccccttcc
    15301 caggtagcgg gaagcatatt tcggcaatcc agagacagcg gcgttatctg gctctggaga
    15361 aagcttataa cagaggataa ccgcgcatgg tgcttggcaa accgcaaaca gacccgactc
    15421 tcgaatggtt cttgtctcat tgccacattc ataagtaccc atccaagagc acgcttattc
    15481 accagggtga aaaagcggaa acgctgtact acatcgttaa aggctctgtg gcagtgctga
    15541 tcaaagacga agagggtaaa gaaatgatcc tctcctatct gaatcagggt gattttattg
    15601 gcgaactggg cctgtttgaa gagggccagg aacgtagcgc atgggtacgt gcgaaaaccg
    15661 cctgtgaagt ggctgaaatt tcgtacaaaa aatttcgcca attgattcag gtaaacccgg
    15721 acattctgat gcgtttgtct gcacagatgg cgcgtcgtct gcaagtcact tcagagaaag
    15781 tgggcaacct ggcgttcctc gacgtgacgg gccgcattgc acagactctg ctgaatctgg
    15841 caaaacaacc agacgctatg actcacccgg acggtatgca aatcaaaatt acccgtcagg
    15901 aaattggtca gattgtcggc tgttctcgtg aaaccgtggg acgcattctg aagatgctgg
    15961 aagatcagaa cctgatctcc gcacacggta aaaccatcgt cgtttacggc actcgttaat
    16021 cccgtcggag tggcgcgtta cctggtagcg cgccattttg tttcccccga tgtggcgcag
    16081 actgatttat caccccgata tcaactatgc acttcgacaa acgctggtgc tatgtttgcc
    16141 cgtggccgtt gggttaatgc ttggcgaatt acgattcggt ctgctcttct ctctcgttcc
    16201 tgcctgttgc aatattgcgg gccttgatac gcctcataaa cgttttttca aacgcttaat
    16261 cattggtgcg tcgctgtttg ccacctgtag cttactgaca cagctgctac tggcaaaaga
    16321 tgttcccctg ccctttttgc tgaccggatt aacgctggta cttggcgtca ctgctgagtt
    16381 ggggccattg cacgcaaaat tgcttcccgc atcgctgctg gcagccattt ttaccctcag
    16441 tctggcggga tacatgccgg tctgggaacc gttgctcatc tatgcgttgg gcactctctg
    16501 gtacggattg tttaactggt tttggttctg gatctggcgc gaacaaccgc tgcgcgagtc
    16561 actaagtctg ctgtaccgtg aactggcaga ttattgtgaa gccaaataca gcctgcttac
    16621 ccagcacacc gaccctgaaa aagcgctgcc gccgctgctg gtgcgccagc aaaaagcggt
    16681 cgatctaatt acccagtgct atcagcaaat gcatatgctt tccgcgcaaa ataatactga
    16741 ctacaagcgg atgctgcgta ttttccagga ggcgctggat ttacaggaac atatttcggt
    16801 cagtttgcat cagccggaag aggtgcaaaa gctggtcgag cgtagccatg cggaagaagt
    16861 tatccgctgg aatgcgcaaa ccgtcgccgc tcgcctgcgc gtgctggctg atgacattct
    16921 ttaccatcgc ctgccaacgc gttttacgat ggaaaagcaa attggcgcac tggaaaaaat
    16981 cgcccgccag catccggata atccggttgg gcaattctgc tactggcatt tcagccgcat
    17041 cgcccgcgtg ctgcgcaccc aaaaaccgct ctatgcacgt gacttactgg ccgataaaca
    17101 gcggcgaatg ccattacttc cggcgctgaa aagttatctg tcactaaagt ctccggcgct
    17161 acgcaatgcc ggacgactca gtgtgatgtt aagcgttgcc agcctgatgg gcaccgcgct
    17221 gcatctgccg aagtcgtact ggatcctgat gacggtattg ctggtgacac aaaatggcta
    17281 tggcgcaacc cgtctgagga ttgtgaatcg ctccgtggga accgtggtcg ggttaatcat
    17341 tgcgggcgtg gcgctgcact ttaaaattcc cgaaggttac accctgacgt tgatgctgat
    17401 taccaccctc gccagctacc tgatattgcg caaaaactac ggctgggcga cggtcggttt
    17461 tactattacc gcagtgtata ccctgcaact attgtggttg aacggcgagc aatacatcct
    17521 tccgcgtctt atcgatacca ttattggttg tttaattgct ttcggcggta ctgtctggct
    17581 gtggccgcag tggcagagcg ggttattgcg taaaaacgcc catgacgctt tagaagccta
    17641 tcaggaagcg attcgcttga ttcttagcga ggatccgcaa cctacgccac tggcctggca
    17701 gcgaatgcgg gtaaatcagg cacataacac tctgtataac tcattgaatc aggcgatgca
    17761 ggaaccggcg tttaacagcc attatctggc agatatgaaa ctgtgggtaa cgcacagcca
    17821 gtttattgtt gagcatatta atgccatgac cacgctggcg cgggaacacc gggcattgcc
    17881 acctgaactg gcacaagagt atttacagtc ttgtgaaatc gccattcagc gttgtcagca
    17941 gcgactggag tatgacgaac cgggtagttc tggcgatgcc aatatcatgg atgcgccgga
    18001 gatgcagccg cacgaaggcg cggcaggtac gctggagcag catttacagc gggttattgg
    18061 tcatctgaac accatgcaca ccatttcgtc gatggcatgg cgtcagcgac cgcatcacgg
    18121 gatttggctg agtcgcaagt tgcgggattc gaaggcgtaa tgcaggctaa accatcggat
    18181 aaggcattca cgccgcatcc gacatctttt gcctgatgct tcgtatttac gccccaacca
    18241 ccttcgccac cgcgtgggcg aaacgttgca tcccttcatc gatatccgca tcttccacca
    18301 ccagcgacgg tgcaaaacgc atcacatccg gtccggcatt cagcaccatt acgccagcct
    18361 ctgcgcccgc atacaggaaa tcacgcgccc gacctttgta ctgtggtttc agctctgcgc
    18421 caatcaacag ccccataccg cgaatatcgc taaacacatc gtactgctga tcgatcttct
    18481 gcagatggtc aacaaaacgc tggcgtttcg cctgaatgcc ttccagcact tcaggggtat
    18541 tgatgatatc aaacgccgcc cccgctactg cacaggccag aggattaccg ccgtaggtgg
    18601 aaccgtgaga accaggatga aacgcagaag caatttctgc cgtggtcagc atggcgctaa
    18661 tcgggaagcc gccgcctaac gctttcgcag aggtcagaat atccggcgtc acgccgtagt
    18721 gcatgtaagc aaacaaatcg ccggtccgcc ccatcccgca ctgtacttca tcaaacacca
    18781 ataatgcctg atgttgatcg cacagctcgc gcaagccctg caaaaactct ggcgtcgctg
    18841 ccgtcacacc gccctcgccc tggatcggct caaccaccac cgcacaggtg tgatcatcca
    18901 tcaccgcttt cactgcgtgg agatcgttaa aaggaacgtg gatgatgtct gccggttttg
    18961 gcccaaagcc gtcggaatat tttggctgcc cgcccaccga gacggtaaac agcgagcgac
    19021 catgaaaagc gttatggaag gcaataattt tggttttgaa cggactatga cgcacacagg
    19081 cgtaatggcg tgccagttta aaggcggttt cgttagcttc cgtgccggag ttcataaaca
    19141 ccacgcgttc ggcaaacgtc gcctcaatca gtttgcgccc aagacgcagc gccggttcat
    19201 tggtgaaaac gttactgata tgccacagag tttcgccctg ggtttttaac gcgttcacca
    19261 gcgcaggatg gcaatggccc aacgccgtaa ctgcaatgcc acccgcgaaa tcgacatact
    19321 ccttgccttg ctgatcccag attcggctgc cctgaccttt taccggaatg aactctgccg
    19381 gagcataaat cggcaggatc acttcatcga aagtcgcgcg tgtaattgct gtttgttcaa
    19441 ttgccatctc atgatcaccc tgttacgcat aaataaatgt gaaattataa tcacaaaata
    19501 tgcataaaaa atcattgaat ggcaatcaga aatcagcgat gcaggaaatt agccagcagt
    19561 tgatgtcctt gttcgctaag aatactttct ggatggaact gcacaccttc cagatcccac
    19621 tggcgatggc gaatccccat aatctcgcgg gtttcgctcc aggccgtcac gtcaaagcat
    19681 gctggtaatg agccaggttc caccaccagc gaatggtagc gtgtcacggt aagtggattt
    19741 gccagccccc gaaatacgcc ctcaccgtta tgtgtaatcg gcgaggtttt gccgtgcatg
    19801 acctttgcgg cgcgcacaac tttgccgcca aatgcctgcg ccattgcctg atgaccgagg
    19861 cagacgccaa gaatcggcaa gcgcccggca tagtggcgaa taacgtcaag ggagatcccg
    19921 gcttcatctg gcgtacaggg gccgggtgat atgacaattt tttgtggttt aagggcgtcg
    19981 atatccgcca gcgtcaacgc atcgttgcgc ttaaccagca catccgcccc cagttcacaa
    20041 aagtactggt agaggttcca ggtaaaagaa tcgtagttat ctataagcag gatcatggcg
    20101 gctccggtac gaaagaaccg cgctatttta ctcagattcc ccggcttcgc ttaccacttt
    20161 gctaaatatc atctgcagcg cggtgagatc ccccattgcc ccactctgat ttgcctgatt
    20221 ccaggcctct ttttcgatac cctgccagct cagttgatac ccggcatgaa tcgccagttg
    20281 ctcgaagaag atccgctgtg ccagaccact tcccacccgg aagggatgca gcacgttgat
    20341 ttcacaatag taatgcgcca gccgctcgac gaacttcgct ttctccaggc caaccagata
    20401 accttcttcc tccagatcct gcatcagggc attgccctct ttttcgatat aagcaaagtg
    20461 gcagaacggc gtatcgccct gataaatatc aacttcacgc agttgccccg cccagtcgaa
    20521 aatatcctga tacagctggc gatggatagt gcgcaaatgc ggtaaaccgc gcaccagcgg
    20581 accaagctca atggtcgcag cacgcagcgc cgtcatttcg taagcggcct gttccagccg
    20641 ctgctgctgg tggatgttca gccggttacg catgatatca aggccaggat aaagatacgg
    20701 atcgcgccct tcgccgaatt tatcgctcat agtgcctcct cagctcttca aggcgcgcta
    20761 aagcctctgc ggcagtaaga gtgactaaag gcatctcgac gccttcaaga cggcgactgg
    20821 cctggaaatt acgattacgc tgaagctccc agagacggga cttttgctta tcggtgagtt
    20881 tcttcacttg atgcctccct gaacgtgtcc atttgccaca agtataagcg gcaaatccgg
    20941 gttacgccgg gagagcagaa gcgcgggcaa gattgcccgc gcgaaaaatc attacggcag
    21001 gactttagcg gaaaggataa ctaccggttt tgacggcaca ttctggtacg gaccaacgtc
    21061 atgagtcggc acctgggaaa tcttatcggc aacgtccatg cctttcacca ctttaccaaa
    21121 taccgcgtaa ccgaaatcac gctgaccatg gtcaaggaag gcgttatcgg caacgttgat
    21181 aaagaactgg ctggtggcgc tgtctttgtc agcggtacgc gccatcgcga tggtgccacg
    21241 cgtgttgcgc aggccgttat cggcttcatt tttgattggc gggtttggtt ttttctgctg
    21301 catctgctcg gtgaaaccgc cgccctgaat cataaagcca ggaatgacgc ggtgaaaggt
    21361 agtgttgtta taaaaaccgc tgttcacata atcgacaaag ttttgcacag acactggcgc
    21421 tttttgttta tccagctcca gttcgatgtt accagctgag gttgtcaaca atacgtgcgg
    21481 gtcccctttc gctgccattg ctgcgggaga aagagcagaa agagcgaaaa cagcagccat
    21541 cgccgccagg gtcgatttga acatgagatt tccttaacaa acagagcaga aaaaaagcaa
    21601 gtgaagagat tctaaatagc cttcaagaac aaggccatcc ctttacctaa ttttacgtat
    21661 ctgaaatata tgtaacctga cgatcacttt ttattccgtg atcaaaatca cctcttaaaa
    21721 tgcaatttag caaccgattg caataaaaca tttaaacaga tcacaaaatc acctaaaatc
    21781 gcccgctcgc ggcatatccc gctatgccat tttatttttg aacgcaggcc aatcatgact
    21841 aacagcaatc gcatcaagct cacatggatt agctttctct cctacgcact gactggtgcg
    21901 ttggttattg tcaccgggat ggtgatggga aatatcgccg attatttcaa tctgcctgtt
    21961 tccagtatga gtaacacctt caccttcctc aacgccggca ttttaatctc tatcttcctc
    22021 aacgcctggc tgatggaaat cgtcccgttg aaaacgcagt tacgttttgg ctttctcctg
    22081 atggtgctgg cggttgccgg tttgatgttc agccacagcc tggcgctgtt ctcggcggcg
    22141 atgttcattc tcggggtggt cagcggcatc accatgtcga ttggtacatt cctggtaaca
    22201 caaatgtatg aagggcgtca gcgcggttcc cgcctgttat ttaccgactc cttcttcagt
    22261 atggctggga tgattttccc aatgatcgcc gcgtttctac tggcgcgcag cattgagtgg
    22321 tactgggttt atgcctgcat cgggctggtg tatgtcgcta tttttattct gaccttcggc
    22381 tgtgagttcc cggcgctggg caaacatgcg ccaaaaacgg atgctccggt agagaaagaa
    22441 aagtggggga tcggcgtact gtttctctct gttgcggcgc tgtgctacat cctcggtcag
    22501 ttaggtttta tctcctgggt gcctgagtat gccaaaggcc tgggcatgag cctgaacgac
    22561 gcgggcacgc tggtgagtaa cttctggatg tcatacatgg tcggcatgtg ggcgttcagc
    22621 tttattcttc gcttctttga tttgcaacgc attctgaccg tactggctgg tctggctgcg
    22681 attctgatgt acgtctttaa caccggaaca ccagcacata tggcgtggtc aattctcgct
    22741 ctgggcttct tctccagcgc gatctatacc accatcatca ccctgggttc acagcagacc
    22801 aaagtaccgt cgccaaaact ggttaacttt gtcctgacct gcggaaccat cggtactatg
    22861 ttgacctttg tggttaccgg cccgattgtt gaacatagcg gtccgcaggc ggcactgctt
    22921 acagcaaacg gtctgtacgc tgtcgtcttt gtgatgtgct tcctgttagg tttcgtcagc
    22981 cgtcaccgtc agcataacac cctgacctct cattaattgc tcatgccgga cggcactctc
    23041 gtcgtccggc cttttcctct cttcccccgc tacgtgcatc tatttctata aacccgttca
    23101 ttttgtccat tttttgcaca aacatgaaat gtcagacaat tccgtgactt aagaaaattt
    23161 atacaaatca gcaatatacc ccttaaggag tatataaagg tgaatttgat ttacatcaat
    23221 aagcggggtt gctgaatcgt taaggtaggc ggtaatagaa aagaaatcga ggcaaaaatg
    23281 agcaaagtca gactcgcaat tatcggtaac ggtatggtcg gccatcgctt tatcgaagat
    23341 cttcttgata aatctgatgc ggccaacttt gatattaccg ttttctgtga agaaccgcgc
    23401 atcgcttatg accgcgtaca cctctcgtct tacttctctc accacaccgc cgaagagctg
    23461 tcgctggtgc gcgaaggctt ctacgagaaa cacggcatca aagttctggt cggcgaacgc
    23521 gctatcacca tcaaccgtca ggagaaggtg attcactcca gcgccgggcg caccgttttt
    23581 tatgacaagc tgatcatggc gaccggttcc tacccgtgga tcccgccaat caaaggttct
    23641 gatactcagg actgctttgt ctatcgcact attgaagacc tcaacgccat tgaatcctgc
    23701 gcccgtcgca gcaaacgcgg tgccgttgtt ggtggcggcc tgttaggtct ggaagccgct
    23761 ggcgcgctga aaaacttagg tattgaaacc cacgttatcg aatttgcccc tatgctgatg
    23821 gcggaacagc ttgatcagat gggtggcgag cagctgcgtc gcaaaatcga aagtatgggc
    23881 gtgcgcgttc acaccagcaa aaacaccctt gagattgtgc aggaaggtgt cgaagcgcgt
    23941 aaaaccatgc gttttgccga cggcagcgaa ctggaagtcg actttatcgt cttctctacc
    24001 ggtatccgtc cgcgcgataa gctggcaacc cagtgtggtc tggacgttgc tccgcgtggg
    24061 ggtatcgtca ttaatgattc ctgccagact tccgatccgg atatctacgc catcggtgaa
    24121 tgcgcaagct ggaacaaccg tgtatttggt ctggtagcac ctggctacaa aatggcgcag
    24181 gtcgccgttg accatattct cggtagcgaa aacgcctttg aaggtgctga ccttagcgcc
    24241 aagctgaaac tgctgggcgt agacgtaggc ggtattggtg atgcgcacgg tcgcacgcct
    24301 ggcgcacgta gctacgttta cctcgacgaa agtaaagaga tctacaaacg cctgattgtc
    24361 agcgaagaca acaaaaccct gctcggtgcg gtactggtgg gcgataccag cgactacggc
    24421 aacctgctgc aactggtgct gaatgctatc gagctgccgg aaaacccgga ttctctgatt
    24481 ctgccggcgc actcgggtag cggtaagccg tctatcggtg ttgataaact gccggacagc
    24541 gcgcaaatct gctcctgctt cgacgtcacc aaaggtgatc tgattgctgc catcaacaaa
    24601 ggctgccaca ccgttgcggc gctgaaagct gaaaccaaag cgggtactgg ctgcggtggc
    24661 tgtatcccgc tggttactca ggtactgaac gcggaactgg cgaaacaggg catcgaagtt
    24721 aacaacaacc tgtgcgaaca ctttgcttat tcgcgtcagg aactgttcca tttgatccgc
    24781 gttgaaggca ttaaaacctt cgaagaactg ctggcgaaac acggcaaagg ctacggttgt
    24841 gaagtttgta aaccgaccgt cggttcgctg ctggcctcct gctggaacga atacattctg
    24901 aagccggaac atactccgct gcaggattct aacgacaact tcctcgctaa catccagaaa
    24961 gacggcacct actcggtgat cccgcgttct ccgggcggtg aaatcacccc ggaagggctg
    25021 atggcagtgg gtcgtatcgc gcgtgaattt aatctctaca ccaaaatcac cggctcccag
    25081 cgcctggcga tgtttggcgc acagaaagac gatctgccgg aaatctggcg tcagctgatt
    25141 gaagctggct tcgaaaccgg tcatgcctat gcgaaagcac tgcgtatggc gaaaacctgc
    25201 gtgggtagca cctggtgccg ctacggtgtt ggcgatagcg tcggcctcgg cgtggaactg
    25261 gaaaaccgct acaaaggcat ccgtacgccg cacaaaatga agttcggtgt ctccggctgt
    25321 acccgtgaat gttcagaagc tcagggtaaa gacgtgggta ttatcgccac tgaaaaaggc
    25381 tggaacctgt atgtttgcgg taacggcggc atgaaaccgc gtcatgcgga tctgctggca
    25441 gcggatatcg atcgcgaaac gctgatcaaa tatctcgacc gcttcatgat gttctacatc
    25501 cgtaccgccg acaaactgac gcgtaccgcg ccgtggttag aaaacctcga aggcggcatc
    25561 gattacctga aagcggtgat cattgacgac aaactggggc tgaacgctca tctggaagaa
    25621 gagatggcgc gcctgcgtga agcggtagtg tgtgagtgga ctgaaacggt caatacaccg
    25681 tccgcgcaga ctcgcttcaa acacttcatc aacagcgaca agcgtgaccc gaacgtgcag
    25741 atggtgccag agcgcgaaca gcaccgtccg gcaacgccgt atgaacgtat cccggtaact
    25801 ctggtggagg acaacgcatg agccagtgga aagacatctg caaaatcgat gacatcctgc
    25861 ctgaaaccgg cgtctgcgcg ctgttaggcg acgagcaggt cgcgattttc cgcccgtatc
    25921 acagcgacca ggtgtttgcg atcagcaaca tcgatccgtt cttcgagtcc agcgtgctgt
    25981 cacgcgggct gattgcggaa caccagggcg agctgtgggt cgccagcccg ctgaaaaaac
    26041 agcgttttcg cttaagtgat ggcttgtgca tggaagacga acagttttcc gtcaaacatt
    26101 acgaagcacg agtgaaagac ggcgtggtgc agctgcgcgg ttaatgtttt aacgggaggc
    26161 gcaatgcctc ccctttttgc atggtcctgt aatgaccttc ggtataatgc aggacatttt
    26221 ttaaactttt tgtt
//
LOCUS       DAZKHF020000038        26137 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2201S31241
            SAMN43267800-rid25106413.denovo.038, whole genome shotgun sequence.
ACCESSION   DAZKHF020000038 DAZKHF020000000
VERSION     DAZKHF020000038.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267800
            Sequence Read Archive: SRR30307263
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 26137)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 26137)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 26137)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350581.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350581.2
            Genome Coverage       :: 151x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:21:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,652
            CDSs (total)                      :: 5,557
            Genes (coding)                    :: 5,320
            CDSs (with protein)               :: 5,320
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 237
            CDSs (without protein)            :: 237
            Pseudo Genes (ambiguous residues) :: 0 of 237
            Pseudo Genes (frameshifted)       :: 102 of 237
            Pseudo Genes (incomplete)         :: 130 of 237
            Pseudo Genes (internal stop)      :: 52 of 237
            Pseudo Genes (multiple problems)  :: 41 of 237
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26137
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267800-rid25106413.denovo.038"
                     /serotype="O 26:H11"
                     /isolate="2201S31241"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-01"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            201..395
                     /gene="bfd"
                     /locus_tag="ACHOSC_RS06430"
                     /old_locus_tag="pgaptmp_001286"
     CDS             201..395
                     /gene="bfd"
                     /locus_tag="ACHOSC_RS06430"
                     /old_locus_tag="pgaptmp_001286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacterioferritin-associated ferredoxin"
                     /protein_id="HGU4547262.1"
                     /translation="MYVCLCNGVSDKKIRQAVRQFHPQSFQQLRKFIPVGNQCGKCIR
                     AAREVMQDELMQMPEFKEIA"
     gene            468..944
                     /gene="bfr"
                     /locus_tag="ACHOSC_RS06435"
                     /old_locus_tag="pgaptmp_001287"
     CDS             468..944
                     /gene="bfr"
                     /locus_tag="ACHOSC_RS06435"
                     /old_locus_tag="pgaptmp_001287"
                     /EC_number="1.16.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004322 - ferroxidase activity [Evidence
                     IEA];
                     GO_function: GO:0008199 - ferric iron binding [Evidence
                     IEA];
                     GO_process: GO:0006879 - intracellular iron ion
                     homeostasis [Evidence IEA];
                     GO_process: GO:0006880 - intracellular sequestering of
                     iron ion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bacterioferritin"
                     /protein_id="HGU4547263.1"
                     /translation="MKGDVKIINYLNKLLGNELVAINQYFLHARMFKNWGLMRLNDVE
                     YHESIDEMKHADKYIERILFLEGIPNLQDLGKLGIGEDVEEMLQSDLRLELEGAKDLR
                     EAIAYADSVHDYVSRDMMIEILADEEGHIDWLETELDLIGKIGLQNYLQSQIKVKD"
     gene            complement(941..1408)
                     /locus_tag="ACHOSC_RS06440"
                     /old_locus_tag="pgaptmp_001288"
     CDS             complement(941..1408)
                     /locus_tag="ACHOSC_RS06440"
                     /old_locus_tag="pgaptmp_001288"
                     /EC_number="3.4.23.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004190 - aspartic-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="A24 family peptidase"
                     /protein_id="HGU4547264.1"
                     /translation="MDANLPFLILYACLSVLLFLWDAKHGLLPDRFTCPLLWSGLLFS
                     QVCNPDGLADALWGAIIGYGTFAVIYWGYRILRHKEGLGYGDVKFLAALGAWHTWTFL
                     PRLVFLAASFACGAVVVGLLMRGKESLKNPLPFGPFLAAAGFVVGWDSLLAGR"
     gene            1787..2098
                     /gene="rpsJ"
                     /locus_tag="ACHOSC_RS06445"
                     /old_locus_tag="pgaptmp_001289"
     CDS             1787..2098
                     /gene="rpsJ"
                     /locus_tag="ACHOSC_RS06445"
                     /old_locus_tag="pgaptmp_001289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014268624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S10"
                     /protein_id="HGU4547265.1"
                     /translation="MQNQRIRIRLKAFDHRLIDQATAEIVETAKRTGAQVRGPIPLPT
                     RKERFTVLISPHVNKDARDQYEIRTHLRLVDIVEPTEKTVDALMRLDLAAGVDVQISL
                     G"
     gene            2131..2760
                     /gene="rplC"
                     /locus_tag="ACHOSC_RS06450"
                     /old_locus_tag="pgaptmp_001290"
     CDS             2131..2760
                     /gene="rplC"
                     /locus_tag="ACHOSC_RS06450"
                     /old_locus_tag="pgaptmp_001290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000579825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L3"
                     /protein_id="HGU4547266.1"
                     /translation="MIGLVGKKVGMTRIFTEDGVSIPVTVIEVEANRVTQVKDLANDG
                     YRAIQVTTGAKKANRVTKPEAGHFAKAGVEAGRGLWEFRLAEGEEFTVGQSISVELFA
                     DVKKVDVTGTSKGKGFAGTVKRWNFRTQDATHGNSLSHRVPGSIGQNQTPGKVFKGKK
                     MAGQMGNERVTVQSLDVVRVDAERNLLLVKGAVPGATGSDLIVKPAVKA"
     gene            2771..3376
                     /gene="rplD"
                     /locus_tag="ACHOSC_RS06455"
                     /old_locus_tag="pgaptmp_001291"
     CDS             2771..3376
                     /gene="rplD"
                     /locus_tag="ACHOSC_RS06455"
                     /old_locus_tag="pgaptmp_001291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010304939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L4"
                     /protein_id="HGU4547267.1"
                     /translation="MELVLKDAQSALTVSETTFGRDFNEALVHQVVVAYAAGARQGTR
                     AQKTRAEVTGSGKKPWRQKGTGRARSGSIKSPIWRSGGVTFAARPQDHSQKVNKKMYR
                     GALKSILSELVRQDRLIVVEKFSVEAPKTKLLAQKLKDMALEDVLIITGELDENLFLA
                     ARNLHKVDVRDATGIDPVSLIAFDKVVMTADAVKQVEEMLA"
     gene            3373..3675
                     /gene="rplW"
                     /locus_tag="ACHOSC_RS06460"
                     /old_locus_tag="pgaptmp_001292"
     CDS             3373..3675
                     /gene="rplW"
                     /locus_tag="ACHOSC_RS06460"
                     /old_locus_tag="pgaptmp_001292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007891652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L23"
                     /protein_id="HGU4547268.1"
                     /translation="MIREERLLKVLRAPHVSEKASTAMEKSNTIVLKVAKDATKAEIK
                     AAVQKLFEVEVEVVNTLVVKGKVKRHGQRIGRRSDWKKAYVTLKEGQNLDFVGGAE"
     gene            3693..4514
                     /gene="rplB"
                     /locus_tag="ACHOSC_RS06465"
                     /old_locus_tag="pgaptmp_001293"
     CDS             3693..4514
                     /gene="rplB"
                     /locus_tag="ACHOSC_RS06465"
                     /old_locus_tag="pgaptmp_001293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001738551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L2"
                     /protein_id="HGU4547269.1"
                     /translation="MAVVKCKPTSPGRRHVVKVVNPELHKGKPFAPLLEKNSKSGGRN
                     NNGRITTRHIGGGHKQAYRIVDFKRNKDGIPAVVERLEYDPNRSANIALVLYKDGERR
                     YILAPKGLKAGDQIQSGVDAAIKPGNTLPMRNIPVGSTVHNVEMKPGKGGQLARSAGT
                     YVQIVARDGAYVTLRLRSGEMRKVEADCRATLGEVGNAEHMLRVLGKAGAARWRGVRP
                     TVRGTAMNPVDHPHGGGEGRNFGKHPVTPWGVQTKGKKTRSNKRTDKFIVRRRSK"
     gene            4531..4809
                     /gene="rpsS"
                     /locus_tag="ACHOSC_RS06470"
                     /old_locus_tag="pgaptmp_001294"
     CDS             4531..4809
                     /gene="rpsS"
                     /locus_tag="ACHOSC_RS06470"
                     /old_locus_tag="pgaptmp_001294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011148791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S19"
                     /protein_id="HGU4547270.1"
                     /translation="MPRSLKKGPFIDLHLLKKVEKAVESGDKKPLRTWSRRSTIFPNM
                     IGLTIAVHNGRQHVPVFVTDEMVGHKLGEFAPTRTYRGHAADKKAKKK"
     gene            4824..5156
                     /gene="rplV"
                     /locus_tag="ACHOSC_RS06475"
                     /old_locus_tag="pgaptmp_001295"
     CDS             4824..5156
                     /gene="rplV"
                     /locus_tag="ACHOSC_RS06475"
                     /old_locus_tag="pgaptmp_001295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000447526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L22"
                     /protein_id="HGU4547271.1"
                     /translation="METIAKHRHARSSAQKVRLVADLIRGKKVSQALDILTYTNKKAA
                     VLVKKVLESAIANAEHNDGADIDDLKVTKIFVDEGPSMKRIMPRAKGRADRILKRTSH
                     ITVVVSDR"
     gene            5174..5875
                     /gene="rpsC"
                     /locus_tag="ACHOSC_RS06480"
                     /old_locus_tag="pgaptmp_001296"
     CDS             5174..5875
                     /gene="rpsC"
                     /locus_tag="ACHOSC_RS06480"
                     /old_locus_tag="pgaptmp_001296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001742302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S3"
                     /protein_id="HGU4547272.1"
                     /translation="MGQKVHPNGIRLGIVKPWNSTWFANTKEFADNLDSDFKVRQYLT
                     KELAKASVSRIVIERPAKSIRVTIHTARPGIVIGKKGEDVEKLRKVVADIAGVPAQIN
                     IAEVRKPELDAKLVADSITSQLERRVMFRRAMKRAVQNAMRLGAKGIKVEVSGRLGGA
                     EIARTEWYREGRVPLHTLRADIDYNTSEAHTTYGVIGVKVWIFKGEILGGMAAVEQPE
                     KPAAQPKKQQRKGRK"
     gene            5888..6298
                     /gene="rplP"
                     /locus_tag="ACHOSC_RS06485"
                     /old_locus_tag="pgaptmp_001297"
     CDS             5888..6298
                     /gene="rplP"
                     /locus_tag="ACHOSC_RS06485"
                     /old_locus_tag="pgaptmp_001297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017805229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L16"
                     /protein_id="HGU4547273.1"
                     /translation="MLQPKRTKFRKMHKGRNRGLAQGTDVSFGSFGLKAVGRGRLTAR
                     QIEAARRAMTRAVKRQGKIWIRVFPDKPITEKPLAVRMGKGKGNVEYWVALIQPGKVL
                     YEMDGVPEELAREAFKLAAAKLPIKTTFVTKTVM"
     gene            6298..6489
                     /gene="rpmC"
                     /locus_tag="ACHOSC_RS06490"
                     /old_locus_tag="pgaptmp_001298"
     CDS             6298..6489
                     /gene="rpmC"
                     /locus_tag="ACHOSC_RS06490"
                     /old_locus_tag="pgaptmp_001298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000644743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L29"
                     /protein_id="HGU4547274.1"
                     /translation="MKAKELREKSVEELNTELLNLLREQFNLRMQAASGQLQQSHLLK
                     QVRRDVARVKTLLNEKAGA"
     gene            6489..6743
                     /gene="rpsQ"
                     /locus_tag="ACHOSC_RS06495"
                     /old_locus_tag="pgaptmp_001299"
     CDS             6489..6743
                     /gene="rpsQ"
                     /locus_tag="ACHOSC_RS06495"
                     /old_locus_tag="pgaptmp_001299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006817275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S17"
                     /protein_id="HGU4547275.1"
                     /translation="MTDKIRTLQGRVVSDKMEKSIVVAIERFVKHPIYGKFIKRTTKL
                     HVHDENNECGIGDVVEIRECRPLSKTKSWTLVRVVEKAVL"
     gene            6908..7279
                     /gene="rplN"
                     /locus_tag="ACHOSC_RS06500"
                     /old_locus_tag="pgaptmp_001300"
     CDS             6908..7279
                     /gene="rplN"
                     /locus_tag="ACHOSC_RS06500"
                     /old_locus_tag="pgaptmp_001300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015817037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L14"
                     /protein_id="HGU4547276.1"
                     /translation="MIQEQTMLNVADNSGARRVMCIKVLGGSHRRYAGVGDIIKITIK
                     EAIPRGKVKKGDVLKAVVVRTKKGVRRPDGSVIRFDGNACVLLNNNSEQPIGTRIFGP
                     VTRELRSEKFMKIISLAPEVL"
     gene            7290..7604
                     /gene="rplX"
                     /locus_tag="ACHOSC_RS06505"
                     /old_locus_tag="pgaptmp_001301"
     CDS             7290..7604
                     /gene="rplX"
                     /locus_tag="ACHOSC_RS06505"
                     /old_locus_tag="pgaptmp_001301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000729185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L24"
                     /protein_id="HGU4547277.1"
                     /translation="MAAKIRRDDEVIVLTGKDKGKRGKVKNVLSSGKVIVEGINLVKK
                     HQKPVPALNQPGGIVEKEAAIQVSNVAIFNAATGKADRVGFRFEDGKKVRFFKSNSET
                     IK"
     gene            7619..8158
                     /gene="rplE"
                     /locus_tag="ACHOSC_RS06510"
                     /old_locus_tag="pgaptmp_001302"
     CDS             7619..8158
                     /gene="rplE"
                     /locus_tag="ACHOSC_RS06510"
                     /old_locus_tag="pgaptmp_001302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001096198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L5"
                     /protein_id="HGU4547278.1"
                     /translation="MAKLHDYYKDEVVKKLMTEFNYNSVMQVPRVEKITLNMGVGEAI
                     ADKKLLDNAAADLAAISGQKPLITKARKSVAGFKIRQGYPIGCKVTLRGERMWEFFER
                     LITIAVPRIRDFRGLSAKSFDGRGNYSMGVREQIIFPEIDYDKVDRVRGLDITITTTA
                     KSDEEGRALLAAFDFPFRK"
     gene            8173..8478
                     /gene="rpsN"
                     /locus_tag="ACHOSC_RS06515"
                     /old_locus_tag="pgaptmp_001303"
     CDS             8173..8478
                     /gene="rpsN"
                     /locus_tag="ACHOSC_RS06515"
                     /old_locus_tag="pgaptmp_001303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005599304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S14"
                     /protein_id="HGU4547279.1"
                     /translation="MAKQSMKAREVKRVALADKYFAKRAELKAIISDVNASDEDRWNA
                     VLKLQTLPRDSSPSRQRNRCRQTGRPHGFLRKFGLSRIKVREAAMRGEIPGLKKASW"
     gene            8512..8904
                     /gene="rpsH"
                     /locus_tag="ACHOSC_RS06520"
                     /old_locus_tag="pgaptmp_001304"
     CDS             8512..8904
                     /gene="rpsH"
                     /locus_tag="ACHOSC_RS06520"
                     /old_locus_tag="pgaptmp_001304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011148781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S8"
                     /protein_id="HGU4547280.1"
                     /translation="MSMQDPIADMLTRIRNGQAANKAAVTMPSSKLKVAIANVLKEEG
                     FIEGFKVEGDTKPELELTLKYFQGKAVVESIQRVSRPGLRIYKRKDELPKVMAGLGIA
                     VVSTSKGVMTDRAARQAGLGGEIICYVA"
     gene            8917..9450
                     /gene="rplF"
                     /locus_tag="ACHOSC_RS06525"
                     /old_locus_tag="pgaptmp_001305"
     CDS             8917..9450
                     /gene="rplF"
                     /locus_tag="ACHOSC_RS06525"
                     /old_locus_tag="pgaptmp_001305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003031129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L6"
                     /protein_id="HGU4547281.1"
                     /translation="MSRVAKAPVVVPAGVDVKINGQVITIKGKNGELTRTLNDAVEVK
                     HADNTLTFGPRDGYADGWAQAGTARALLNSMVIGVTEGFTKKLQLVGVGYRAAVKGNV
                     INLSLGFSHPVDHQLPAGITAECPTQTEIVLKGADKQVIGQVAADLRAYRRPEPYKGK
                     GVRYADEVVRTKEAKKK"
     gene            9460..9813
                     /gene="rplR"
                     /locus_tag="ACHOSC_RS06530"
                     /old_locus_tag="pgaptmp_001306"
     CDS             9460..9813
                     /gene="rplR"
                     /locus_tag="ACHOSC_RS06530"
                     /old_locus_tag="pgaptmp_001306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005970258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L18"
                     /protein_id="HGU4547282.1"
                     /translation="MDKKSARIRRATRARRKLQELGATRLVVHRTPRHIYAQVIAPNG
                     SEVLVAASTVEKAIAEQLKYTGNKDAAAAVGKAVAERALEKGIKDVSFDRSGFQYHGR
                     VQALADAAREAGLQF"
     gene            9828..10331
                     /gene="rpsE"
                     /locus_tag="ACHOSC_RS06535"
                     /old_locus_tag="pgaptmp_001307"
     CDS             9828..10331
                     /gene="rpsE"
                     /locus_tag="ACHOSC_RS06535"
                     /old_locus_tag="pgaptmp_001307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004106339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S5"
                     /protein_id="HGU4547283.1"
                     /translation="MAHIEKQAGELQEKLIAVNRVSKTVKGGRIFSFTALTVVGDGNG
                     RVGFGYGKAREVPAAIQKAMEKARRNMINVALNNGTLQHPVKGVHTGSRVFMQPASEG
                     TGIIAGGAMRAVLEVAGVHNVLAKAYGSTNPINVVRATIDGLENMNSPEMVAAKRGKS
                     VEEILGK"
     gene            10335..10514
                     /gene="rpmD"
                     /locus_tag="ACHOSC_RS06540"
                     /old_locus_tag="pgaptmp_001308"
     CDS             10335..10514
                     /gene="rpmD"
                     /locus_tag="ACHOSC_RS06540"
                     /old_locus_tag="pgaptmp_001308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012147215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L30"
                     /protein_id="HGU4547284.1"
                     /translation="MAKTIKITQTRSAIGRLPKHKATLLGLGLRRIGHTVEREDTPAI
                     RGMINAVSFMVKVEE"
     gene            10518..10952
                     /gene="rplO"
                     /locus_tag="ACHOSC_RS06545"
                     /old_locus_tag="pgaptmp_001309"
     CDS             10518..10952
                     /gene="rplO"
                     /locus_tag="ACHOSC_RS06545"
                     /old_locus_tag="pgaptmp_001309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011095501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L15"
                     /protein_id="HGU4547285.1"
                     /translation="MRLNTLSPAEGSKKAGKRLGRGIGSGLGKTGGRGHKGQKSRSGG
                     GVRRGFEGGQMPLYRRLPKFGFTSRKAAITAEVRLSDLAKVEGGVVDLNTLKAANIIG
                     IQIEFAKVILAGEVTTPVTVRGLRVTKGARAAIEAAGGKIEE"
     gene            10960..12291
                     /gene="secY"
                     /locus_tag="ACHOSC_RS06550"
                     /old_locus_tag="pgaptmp_001310"
     CDS             10960..12291
                     /gene="secY"
                     /locus_tag="ACHOSC_RS06550"
                     /old_locus_tag="pgaptmp_001310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004868351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0015031 - protein transport [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecY"
                     /protein_id="HGU4547286.1"
                     /translation="MAKQPGLDFQSAKGGLGELKRRLLFVIGALIVFRIGSFIPIPGI
                     DAAVLAKLLEQQRGTIIEMFNMFSGGALSRASIFALGIMPYISASIIIQLLTVVHPTL
                     AEIKKEGESGRRKISQYTRYGTLVLAIFQSIGIATGLPNMPGMQGLVINPGFAFYFTA
                     VVSLVTGTMFLMWLGEQITERGIGNGISIIIFAGIVAGLPPAIAHTIEQARQGDLHFL
                     VLLLVAVLVFAVTFFVVFVERGQRRIVVNYAKRQQGRRVYAAQSTHLPLKVNMAGVIP
                     AIFASSIILFPATIASWFGGGTGWNWLTTISLYLQPGQPLYVLLYASAIIFFCFFYTA
                     LVFNPRETADNLKKSGAFVPGIRPGEQTAKYIDKVMTRLTLVGALYITFICLIPEFMR
                     DAMKVPFYFGGTSLLIVVVVIMDFMAQVQTLMMSSQYESALKKANLKGYGR"
     gene            12323..12439
                     /gene="rpmJ"
                     /locus_tag="ACHOSC_RS06555"
                     /old_locus_tag="pgaptmp_001311"
     CDS             12323..12439
                     /gene="rpmJ"
                     /locus_tag="ACHOSC_RS06555"
                     /old_locus_tag="pgaptmp_001311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020322760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L36"
                     /protein_id="HGU4547287.1"
                     /translation="MKVRASVKKLCRNCKIVKRDGVIRVICSAEPKHKQRQG"
     gene            12586..12942
                     /gene="rpsM"
                     /locus_tag="ACHOSC_RS06560"
                     /old_locus_tag="pgaptmp_001312"
     CDS             12586..12942
                     /gene="rpsM"
                     /locus_tag="ACHOSC_RS06560"
                     /old_locus_tag="pgaptmp_001312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019935869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S13"
                     /protein_id="HGU4547288.1"
                     /translation="MARIAGINIPDHKHAVIALTSIYGVGKTRSKAILAAAGIAEDVK
                     ISELSEGQIDTLRDEVAKFVVEGDLRREISMSIKRLMDLGCYRGLRHRRGLPVRGQRT
                     KTNARTRKGPRKPIKK"
     gene            12959..13348
                     /gene="rpsK"
                     /locus_tag="ACHOSC_RS06565"
                     /old_locus_tag="pgaptmp_001313"
     CDS             12959..13348
                     /gene="rpsK"
                     /locus_tag="ACHOSC_RS06565"
                     /old_locus_tag="pgaptmp_001313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001029758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S11"
                     /protein_id="HGU4547289.1"
                     /translation="MAKAPIRARKRVRKQVSDGVAHIHASFNNTIVTITDRQGNALGW
                     ATAGGSGFRGSRKSTPFAAQVAAERCADAVKEYGIKNLEVMVKGPGPGRESTIRALNA
                     AGFRITNITDVTPIPHNGCRPPKKRRV"
     gene            13382..14002
                     /gene="rpsD"
                     /locus_tag="ACHOSC_RS06570"
                     /old_locus_tag="pgaptmp_001314"
     CDS             13382..14002
                     /gene="rpsD"
                     /locus_tag="ACHOSC_RS06570"
                     /old_locus_tag="pgaptmp_001314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004410410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S4"
                     /protein_id="HGU4547290.1"
                     /translation="MARYLGPKLKLSRREGTDLFLKSGVRAIDTKCKIEQAPGQHGAR
                     KPRLSDYGVQLREKQKVRRIYGVLERQFRNYYKEAARLKGNTGENLLALLEGRLDNVV
                     YRMGFGATRAEARQLVSHKAIMVNGRVVNIASYQVSPNDVVSIREKAKKQSRVKAALE
                     LAEQREKPTWLEVDAGKMEGTFKRKPERSDLSADINEHLIVELYSK"
     gene            14028..15017
                     /gene="rpoA"
                     /locus_tag="ACHOSC_RS06575"
                     /old_locus_tag="pgaptmp_001315"
     CDS             14028..15017
                     /gene="rpoA"
                     /locus_tag="ACHOSC_RS06575"
                     /old_locus_tag="pgaptmp_001315"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005970247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit alpha"
                     /protein_id="HGU4547291.1"
                     /translation="MQGSVTEFLKPRLVDIEQVSSTHAKVTLEPLERGFGHTLGNALR
                     RILLSSMPGCAVTEVEIDGVLHEYSTKEGVQEDILEILLNLKGLAVRVQGKDEVILTL
                     NKSGIGPVTAADITHDGDVEIVKPQHVICHLTDENASISMRIKVQRGRGYVPASTRIH
                     SEEDERPIGRLLVDACYSPVERIAYNVEAARVEQRTDLDKLVIEMETNGTIDPEEAIR
                     RAATILAEQLEAFVDLRDVRQPEVKEEKPEFDPILLRPVDDLELTVRSANCLKAEAIH
                     YIGDLVQRTEVELLKTPNLGKKSLTEIKDVLASRGLSLGMRLENWPPASIADE"
     gene            15058..15441
                     /gene="rplQ"
                     /locus_tag="ACHOSC_RS06580"
                     /old_locus_tag="pgaptmp_001316"
     CDS             15058..15441
                     /gene="rplQ"
                     /locus_tag="ACHOSC_RS06580"
                     /old_locus_tag="pgaptmp_001316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L17"
                     /protein_id="HGU4547292.1"
                     /translation="MRHRKSGRQLNRNSSHRQAMFRNMAGSLVRHEIIKTTLPKAKEL
                     RRVVEPLITLAKTDSVANRRLAFARTRDNEIVAKLFNELGPRFASRAGGYTRILKCGF
                     RAGDNAPMAYIELVDRSEKAEAAAE"
     gene            15548..15916
                     /locus_tag="ACHOSC_RS06585"
                     /old_locus_tag="pgaptmp_001317"
     CDS             15548..15916
                     /locus_tag="ACHOSC_RS06585"
                     /old_locus_tag="pgaptmp_001317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1992 domain-containing protein"
                     /protein_id="HGU4547293.1"
                     /translation="MWLLDQWAERHIAEAQAKGEFDNLAGSGEPLILDDDSHVPPELR
                     AGYRLLKNAGCLPPELEQRREAIQLLDILRGIRHDDPQYQEVSRRLSLLELKLRQAGL
                     STDFLRGDYADKLLDKINDN"
     gene            15927..16352
                     /gene="zntR"
                     /locus_tag="ACHOSC_RS06590"
                     /old_locus_tag="pgaptmp_001318"
     CDS             15927..16352
                     /gene="zntR"
                     /locus_tag="ACHOSC_RS06590"
                     /old_locus_tag="pgaptmp_001318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Zn(2+)-responsive transcriptional regulator"
                     /protein_id="HGU4547294.1"
                     /translation="MYRIGELAKMAEVTPDTIRYYEKQQMMEHEVRTEGGFRLYTESD
                     LQRLKFIRHARQLGFSLESIRELLSIRIDPEHHTCQESKGIVQERLQEVEARIAELQS
                     MQRSLQRLNDACCGTAHSSVYCSILEALEQGASGVKSGC"
     gene            16408..16626
                     /gene="arfA"
                     /locus_tag="ACHOSC_RS06595"
                     /old_locus_tag="pgaptmp_001319"
     CDS             16408..16626
                     /gene="arfA"
                     /locus_tag="ACHOSC_RS06595"
                     /old_locus_tag="pgaptmp_001319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alternative ribosome-rescue factor ArfA"
                     /protein_id="HGU4547295.1"
                     /translation="MSRYQHTKGQIKDNAIEALLHDPLFRQRVEKNKKGKGSYMRKGK
                     HGNRGNWEASGKKVNHFFTTGLLLSGAC"
     gene            complement(16623..17033)
                     /gene="mscL"
                     /locus_tag="ACHOSC_RS06600"
                     /old_locus_tag="pgaptmp_001320"
     CDS             complement(16623..17033)
                     /gene="mscL"
                     /locus_tag="ACHOSC_RS06600"
                     /old_locus_tag="pgaptmp_001320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="large-conductance mechanosensitive channel
                     protein MscL"
                     /protein_id="HGU4547296.1"
                     /translation="MSIIKEFREFAMRGNVVDLAVGVIIGAAFGKIVSSLVADIIMPP
                     LGLLIGGIDFKQFAVTLRDAQGDIPAVVMHYGVFIQNVFDFLIVAFAIFMAIKLINKL
                     NRKKEEPAAAPAPTKEEVLLAEIRDLLKEQNNRS"
     gene            complement(17163..18539)
                     /gene="trkA"
                     /locus_tag="ACHOSC_RS06605"
                     /old_locus_tag="pgaptmp_001321"
     CDS             complement(17163..18539)
                     /gene="trkA"
                     /locus_tag="ACHOSC_RS06605"
                     /old_locus_tag="pgaptmp_001321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004125888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015079 - potassium ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Trk system potassium transporter TrkA"
                     /protein_id="HGU4547297.1"
                     /translation="MKIIILGAGQVGGTLAENLVGENNDITVVDTNGERLRTLQDKFD
                     LRVVQGHGSHPRVLREAGADDADMLVAVTSSDETNMVACQVAYSLFNTPNRIARIRSP
                     DYVRDADKLFHSDAVPIDHLIAPEQLVIDNIYRLIEYPGALQVVNFAEGKVSLAVVKA
                     YYGGPLIGNALSTMREHMPHIDTRVAAIFRHDRPIRPQGSTIVEAGDEVFFIAASQHI
                     RAVMSELQRLEKPYKRIMLVGGGNIGAGLARRLEKDYSVKLIERNQQRAAELAEKLQN
                     TIVFFGDASDQELLAEEHIDQVDLFIAVTNDDEANIMSAMLAKRMGAKKVMVLIQRRA
                     YVDLVQGSVIDIAISPQQATISALLSHVRKADIVGVSSLRRGVAEAIEAVAHGDESTS
                     RVVGRVIDEIKLPPGTIIGAVVRGNDVMIANDNLRIEQGDHVIMFLTDKKFITDVERL
                     FQPSPFFL"
     gene            complement(18561..19850)
                     /gene="rsmB"
                     /locus_tag="ACHOSC_RS06610"
                     /old_locus_tag="pgaptmp_001322"
     CDS             complement(18561..19850)
                     /gene="rsmB"
                     /locus_tag="ACHOSC_RS06610"
                     /old_locus_tag="pgaptmp_001322"
                     /EC_number="2.1.1.176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0008649 - rRNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0001510 - RNA methylation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(967)-C(5))-methyltransferase
                     RsmB"
                     /protein_id="HGU4547298.1"
                     /translation="MKKQRNLRSMAAQAVEQIVEQGQSLSNILPPLQQKVSDKDKALL
                     QELCFGVLRTLSQLDWLINKLMARPMTGKQRTVHYLIMVGVYQLLYTRIPPHAALAET
                     VEGAVAIKRPQLKGLINGVLRQFQRQQEELLAGFYASDARYLHPSWLLKRLQKAYPEQ
                     WQSIVEANNQRPPMWLRVNRTHHSRDSWLALLDEAGMKGFPHADYPDAVRLETPAPVH
                     ALPGFEDGWVTVQDASAQGCMTWLAPQNGEHILDLCAAPGGKTTHILEVAPEAQVVAV
                     DIDEQRLSRVYDNLKRLGMKATVKQGDGRYPSQWCGEQQFDRILLDAPCSATGVIRRH
                     PDIKWLRRDRDIPELAQLQSEILDAIWPHLKSGGTLVYATCSVLPEENSLQIKAFLQR
                     TADAELCETGTPEQPGKQNLPGTEEGDGFFYAKLIKK"
     gene            complement(19896..20843)
                     /gene="fmt"
                     /locus_tag="ACHOSC_RS06615"
                     /old_locus_tag="pgaptmp_001323"
     CDS             complement(19896..20843)
                     /gene="fmt"
                     /locus_tag="ACHOSC_RS06615"
                     /old_locus_tag="pgaptmp_001323"
                     /EC_number="2.1.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004479 - methionyl-tRNA formyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0071951 - conversion of methionyl-tRNA to
                     N-formyl-methionyl-tRNA [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionyl-tRNA formyltransferase"
                     /protein_id="HGU4547299.1"
                     /translation="MSESLRIIFAGTPDFAARHLDALLSSGHNVVGVFTQPDRPAGRG
                     KKLMPSPVKVLAEEKGLPVFQPVSLRPQESQQLVADLQADVMVVVAYGLILPKAVLEM
                     PRLGCINVHGSLLPRWRGAAPIQRSLWAGDAETGVTIMQMDVGLDTGDMLYKLSCPIT
                     AEDTSGTLYDKLAELGPQGLITTLKQLADGTAKPEVQDETLVTYAEKLSKEEARIDWS
                     LSAAQLERCIRAFNPWPMSWLEIEGQPVKVWKASVIDTATNAAPGTILEANKQGIQVA
                     TGDGILNLLSLQPAGKKAMSAQDLLNSRREWFVPGNRLV"
     gene            complement(20858..21367)
                     /gene="def"
                     /locus_tag="ACHOSC_RS06620"
                     /old_locus_tag="pgaptmp_001324"
     CDS             complement(20858..21367)
                     /gene="def"
                     /locus_tag="ACHOSC_RS06620"
                     /old_locus_tag="pgaptmp_001324"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042586 - peptide deformylase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HGU4547300.1"
                     /translation="MSVLQVLHIPDERLRKVAKPVEEVNAEIQRIVDDMFETMYAEEG
                     IGLAATQVDIHQRIIVIDVSENRDERLVLINPELLEKSGETGIEEGCLSIPEQRALVP
                     RAEKVKIRALDRDGKPFELEAEGLLAICIQHEMDHLVGKLFMDYLSPLKQQRIRQKVE
                     KLDRLKARA"
     gene            21497..22621
                     /gene="dprA"
                     /locus_tag="ACHOSC_RS06625"
                     /old_locus_tag="pgaptmp_001325"
     CDS             21497..22621
                     /gene="dprA"
                     /locus_tag="ACHOSC_RS06625"
                     /old_locus_tag="pgaptmp_001325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009294 - DNA-mediated transformation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-protecting protein DprA"
                     /protein_id="HGU4547301.1"
                     /translation="MVDTEIWLRLMSISSLYGDDMVRIAHWLAKQSQIDAVGLQQTGL
                     TLRQAQRFLSFPRKSIESSLCWLEQPNHHLIPADSEFYPPQLLATTDYPGALFVEGEL
                     HALHSFQLAVVGSRAHSWYGERWGRLFCETLATRGVTITSGLARGIDGVAHKAALQVN
                     GVSIAVLGNGLNTIHPRRHARLAASLLEHGGALVSEFPLDVPPLAYNFPRRNRIISGL
                     SKGVLVVEAALRSGSLVTARCALEQGREVFALPGPIGNPGSEGPHWLIKQGAILVTEP
                     EEILENLQFGLHWLPDAPENSFYSPDQQDVALPFPELLANVGDEVTPVDVVAERAGQP
                     VPEVVTQLLELELAGWIAAVPGGYVRLRRACHVRRTNVFV"
     gene            22593..23066
                     /gene="smg"
                     /locus_tag="ACHOSC_RS06630"
                     /old_locus_tag="pgaptmp_001326"
     CDS             22593..23066
                     /gene="smg"
                     /locus_tag="ACHOSC_RS06630"
                     /old_locus_tag="pgaptmp_001326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005134083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF494 family protein Smg"
                     /protein_id="HGU4547302.1"
                     /translation="MFDVLMYLFETYIHTEAELRVDQDKLEQDLTDAGFDREDIYNAL
                     LWLEKLADYQEGLAEPMQLASDPLSMRIYTPEECERLDASCRGFLLFLEQIQVLNLET
                     REMVIERVLALDTAEFDLEDLKWVILMVLFNIPGCENAYQQMEELLFEVNEGMLH"
     gene            23095..23637
                     /locus_tag="ACHOSC_RS06635"
                     /old_locus_tag="pgaptmp_001327"
     CDS             23095..23637
                     /locus_tag="ACHOSC_RS06635"
                     /old_locus_tag="pgaptmp_001327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005694 - chromosome [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="topoisomerase DNA-binding C4 zinc finger
                     domain-containing protein"
                     /protein_id="HGU4547303.1"
                     /translation="MAKSALFTVRNNESCPKCGAELVIRSGKHGPFLGCSQYPACDYV
                     RPLKSSADGHIVKVLEGQVCLACGANLVLRQGRFGMFIGCSNYPECEHTELIDKPDET
                     AITCPQCRTGHLVQRRSRYGKTFHSCDRYPECQFAINFKPIAGECPECHYPLLIEKKT
                     AQGVKHFCASKQCGKPVSAE"
     gene            23642..24214
                     /gene="tsaC"
                     /locus_tag="ACHOSC_RS06640"
                     /old_locus_tag="pgaptmp_001328"
     CDS             23642..24214
                     /gene="tsaC"
                     /locus_tag="ACHOSC_RS06640"
                     /old_locus_tag="pgaptmp_001328"
                     /EC_number="2.7.7.87"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003725 - double-stranded RNA binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0002949 - tRNA threonylcarbamoyladenosine
                     modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonylcarbamoyladenylate synthase type 1
                     TsaC"
                     /protein_id="HGU4547304.1"
                     /translation="MNNNLQGDAIAAAIDVLNEERVIAYPTEAVFGVGCDPDSETAVM
                     RLLELKQRPVDKGLILIAANYEQLKPYIDDTMLTDAQRETIFSRWPGPVTFVFPAPAT
                     TPRWLTGRFDSLAVRVTDHPLVVALCQAYGKPLVSTSANLSGLPPCRTVDEVRAQFGA
                     AFPVVPGETGGRLNPSEIRDALTGELFRQG"
     gene            24219..25037
                     /gene="aroE"
                     /locus_tag="ACHOSC_RS06645"
                     /old_locus_tag="pgaptmp_001329"
     CDS             24219..25037
                     /gene="aroE"
                     /locus_tag="ACHOSC_RS06645"
                     /old_locus_tag="pgaptmp_001329"
                     /EC_number="1.1.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709069.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004764 - shikimate 3-dehydrogenase
                     (NADP+) activity [Evidence IEA];
                     GO_process: GO:0019632 - shikimate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate dehydrogenase"
                     /protein_id="HGU4547305.1"
                     /translation="METYAVFGNPIAHSKSPFIHQQFAQQLNIEHPYGRVLAPINDFI
                     NTLNAFFRAGGKGANVTVPFKEEAFARADELTERAALAGAVNTLKRLEDGRLLGDNTD
                     GIGLLSDLERLSFIRPGLRILLIGAGGASRGVLLPLLSLDCAVTITNRTVSRAEELAK
                     LFAHTGSIQALGMDELEGHEFDLIINATSSGISGDIPAIPSSLIHPGIYCYDMFYQKG
                     KTPFLAWCEQRGSKRNADGLGMLVAQAAHAFLLWHGVLPDVEPVIKQLQEELSA"
     gene            25034..25291
                     /locus_tag="ACHOSC_RS06650"
                     /old_locus_tag="pgaptmp_001330"
     CDS             25034..25291
                     /locus_tag="ACHOSC_RS06650"
                     /old_locus_tag="pgaptmp_001330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1488 domain-containing protein"
                     /protein_id="HGU4547306.1"
                     /translation="MNQAIQFPDREEWDENKKCVCFPALVNGMQLTCAISGESLAYRF
                     TGDTPEQWLASFRQHRWDLEEEAENLIQEQSEDDQGWVWLP"
     gene            complement(25267..25821)
                     /locus_tag="ACHOSC_RS06655"
                     /old_locus_tag="pgaptmp_001331"
     CDS             complement(25267..25821)
                     /locus_tag="ACHOSC_RS06655"
                     /old_locus_tag="pgaptmp_001331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma carbonic anhydrase family protein"
                     /protein_id="HGU4547307.1"
                     /translation="MSDVLRPYRDLFPQIGQRVMIDDSSVVIGDVRLADDVGIWPLVV
                     IRGDVHYVQIGARTNIQDGSMLHVTHKSSYNPDGNPLTIGEDVTVGHKVMLHGCTIGN
                     RVLVGMGSILLDGAIVEDDVMIGAGSLVPQNKRLESGYLYLGSPVKQIRPLSDEEKAG
                     LRYSANNYVKWKDEYLDQGNQTQP"
BASE COUNT         6721 a         6202 c         6777 g         6437 t
ORIGIN      
        1 gctaaagttc tgggctaatt acacgttaat tagttttgaa ttgaaaaggg cgcttcggcg
       61 ccctttttgc tgcccgtaat ttgagctcga aatatttagt cgtaattttg ctcgctgaac
      121 tatggcgcaa ataaaactgt gctattgtaa tcataaccat tctcatttac actttgcgca
      181 gaatttgaac gggagcaatc atgtacgttt gtttgtgtaa tggtgtaagt gacaaaaaaa
      241 ttcgccaggc agtccgtcag tttcatccgc aatctttcca acaattgcgt aagtttattc
      301 ctgtgggaaa tcaatgcggt aagtgcattc gcgccgcgcg cgaagtgatg caggacgagt
      361 tgatgcagat gccagaattt aaagagattg cctgaagacc acgcttattt tttgacatcc
      421 cgctagcccc atctacgctc taatagtgga agcggaggga ctataaaatg aaaggtgatg
      481 ttaaaatcat aaattatctc aataaactat tgggaaatga gcttgtcgca atcaatcagt
      541 attttctcca tgcccggatg tttaaaaact ggggcctgat gcgtctcaat gacgtcgaat
      601 accatgaatc catcgatgag atgaaacacg ctgataaata tatcgagcgt attttgtttt
      661 tagaaggaat ccccaactta caggatttag gcaaactggg gattggtgaa gatgtcgagg
      721 agatgctgca atccgacctg cgattagaac tggaaggcgc gaaggatctc cgtgaggcta
      781 tcgcgtatgc cgacagcgtt catgactatg ttagccgtga tatgatgatc gagatccttg
      841 ccgatgaaga aggtcatatt gactggctcg aaactgagct ggatctcatc ggtaaaatcg
      901 gtttacaaaa ttacctgcaa tcacaaataa aagttaaaga ttaacggcct gctagcaaac
      961 tatcccaacc tacaacgaaa cccgcagccg ccagaaatgg cccaaacggc agcgggtttt
     1021 ttaatgactc ttttcctctc atgagcagac caacaactac ggctccgcaa gcgaatgagg
     1081 cagcaaggaa aaccagtcgt ggcaagaagg tccaggtatg ccaggcacca agggcggcga
     1141 gaaacttcac atcaccatag cctaatcctt ctttatgacg cagtatgcga tacccccagt
     1201 aaatgacagc gaatgtgccg taaccaataa tcgcccccca taacgcatcg gctaaaccgt
     1261 caggattaca aacctgagag aacaaaagac ctgaccagag taacgggcag gtaaatctgt
     1321 caggtaacaa gccatgcttt gcatcccata aaaaaagtag gacagataaa caagcgtaca
     1381 aaatcagaaa gggaaggtta gcgtccataa aacatcctct gtaattggag tatcaccatc
     1441 gaataactcc ggatgctcag caacagagaa atcgctgatt tgttaacctg gttccggtga
     1501 aaaacgctat cttcacaacc ttacaaattt cgtggaatac agctcgcaat ctgcgcgttg
     1561 cgattaattt ctacccgatg cttgcgtcag gctcagattt gcgtataatg cgcgggcttg
     1621 tcgtagttga cagcaggttc aatctgaacc tcagttagcc gaatttggct acctaacaat
     1681 gctcccaatc ggggagctac gtaagaacgg ttacactctc ccatcaatcg taatgggtct
     1741 gaggagtaat cattttcgtt tataaaataa ttggagctct ggtctcatgc agaaccaaag
     1801 aatccgtatc cgcctgaaag cgtttgatca tcgtctgatc gatcaagcaa ccgcggaaat
     1861 cgtcgagact gccaagcgca ctggtgcgca ggtccgtggt ccgatcccgc tgccgacacg
     1921 caaagagcgc ttcactgttc tgatctcccc gcacgtcaac aaagacgcgc gcgatcagta
     1981 cgaaatccgt actcacttgc gtctggttga catcgttgag ccaaccgaga aaaccgttga
     2041 tgctctgatg cgtctggatc tggctgccgg tgtagacgtg cagatcagcc tgggttaatc
     2101 aggtcattga gcgattgaga ggttgaaaca atgattggtt tagtcggtaa aaaagtgggt
     2161 atgacccgta tcttcacaga agacggcgtt tctatcccag taaccgtaat cgaagttgaa
     2221 gcaaaccgcg ttactcaggt taaagacctg gctaacgatg gctaccgtgc tattcaggtg
     2281 accaccggtg ctaaaaaagc taaccgtgtg accaagcctg aagctggcca cttcgctaaa
     2341 gctggcgtag aagctggccg tggtctgtgg gaattccgcc tggctgaagg cgaagagttc
     2401 actgtaggtc agagcattag cgttgaactg tttgctgacg ttaaaaaagt tgacgtaact
     2461 ggcacctcta aaggtaaagg tttcgcaggt accgttaagc gctggaactt ccgtacccag
     2521 gacgctactc acggtaactc cttgtctcac cgcgttccgg gttctatcgg tcagaaccag
     2581 actccgggca aagtgttcaa aggcaagaaa atggcaggtc agatgggtaa cgaacgtgta
     2641 accgttcaga gccttgacgt agtacgcgtt gacgctgagc gcaacctgct gctggttaaa
     2701 ggtgctgtcc cgggtgcaac cggtagcgac ctgatcgtta aaccagctgt gaaggcgtaa
     2761 ggagatagca atggaattag tattgaaaga cgcgcagagc gcgctgactg tttccgaaac
     2821 taccttcggt cgtgatttca acgaagcgct ggttcaccag gttgttgttg cttatgcagc
     2881 tggtgctcgt cagggtactc gtgctcagaa gactcgtgct gaagtaactg gttctggtaa
     2941 aaaaccgtgg cgccagaaag gcaccggccg tgcgcgttct ggttctatca agagcccgat
     3001 ctggcgttct ggtggcgtga cctttgctgc tcgtccgcag gaccacagtc aaaaagttaa
     3061 caagaagatg taccgcggcg cgctgaaaag catcctgtcc gaactggtac gtcaggatcg
     3121 tctgatcgtt gtcgagaagt tctctgtaga agcgccgaaa actaagctgc tggcacagaa
     3181 actgaaagac atggctctgg aagatgtgct gatcatcacc ggtgagctgg acgaaaacct
     3241 gttcctggct gcgcgcaacc tgcacaaggt tgacgtacgc gatgcaactg gtatcgaccc
     3301 ggttagcctg atcgccttcg acaaagtcgt aatgactgct gatgctgtta agcaagttga
     3361 ggagatgctg gcatgattcg tgaagaacgt ctgctgaagg tgctgcgtgc accgcacgtt
     3421 tctgaaaaag cgtctactgc gatggaaaaa tccaacacca tcgtactcaa agttgctaaa
     3481 gacgcgacca aagcagaaat caaagctgct gtgcagaaac tgtttgaagt cgaagtcgaa
     3541 gtcgttaaca ccctggtagt taaagggaaa gttaaacgtc acggacagcg tatcggtcgt
     3601 cgtagcgact ggaaaaaagc ttacgtcacc ctgaaagaag gccagaatct ggacttcgtt
     3661 ggcggcgctg agtaagtcgg aggagtaata caatggcagt tgttaaatgt aaaccgacat
     3721 ctccgggtcg tcgccacgta gttaaagtgg ttaaccctga gctgcacaag ggcaaacctt
     3781 ttgctccgtt gctggaaaaa aacagcaaat ccggtggtcg taacaacaat ggccgtatca
     3841 ccactcgtca tatcggtggt ggccacaagc aggcttaccg tattgttgac ttcaaacgca
     3901 acaaagacgg tatcccggca gttgttgaac gtcttgagta cgatccgaac cgttccgcga
     3961 acatcgcgct ggttctgtac aaagacggtg aacgccgtta catcctggcc cctaaaggcc
     4021 tgaaagctgg cgaccagatt cagtctggcg ttgatgctgc aatcaaacca ggtaacaccc
     4081 tgccgatgcg caacatcccg gttggttcta ctgttcataa cgtagaaatg aaaccaggta
     4141 aaggcggtca gctggcacgt tccgctggta cttacgttca gatcgttgct cgtgatggtg
     4201 cttatgtcac cctgcgtctg cgttctggtg aaatgcgtaa agtagaagca gactgccgtg
     4261 caactctggg cgaagttggc aatgctgagc atatgctgcg cgttctgggt aaagcaggtg
     4321 ctgcacgctg gcgtggtgtt cgtccgaccg ttcgcggtac cgcgatgaac ccggtagacc
     4381 acccacatgg tggtggtgaa ggtcgtaact ttggtaagca cccggtaact ccgtggggcg
     4441 ttcagaccaa aggtaagaag acccgcagca acaagcgtac tgataaattc atcgtacgtc
     4501 gccgtagcaa ataattttag aggataagcc atgccacgtt ctctcaagaa aggtcctttt
     4561 attgacctgc acttgctgaa gaaggtagag aaagcggtgg aaagcggaga caagaagccc
     4621 ctgcgcactt ggtcccgtcg ttcaacgatc tttcctaaca tgatcggttt gaccatcgct
     4681 gtccataatg gtcgtcagca cgttccggta tttgtaaccg acgaaatggt cggtcacaaa
     4741 ctgggtgaat tcgcaccgac tcgtacttat cgcggccacg ctgctgataa aaaagcgaag
     4801 aagaaataag gtaggaggaa gagatggaaa ctatcgctaa acatcgccat gctcgttctt
     4861 ctgctcagaa ggttcgcctt gttgctgacc tgattcgcgg taagaaagtg tcgcaggctc
     4921 tggatatttt gacctacacc aacaagaaag cggctgtact ggtcaagaaa gttctggaat
     4981 ctgccattgc taacgctgaa cacaacgatg gcgctgacat tgacgatctg aaagttacga
     5041 aaattttcgt agacgaaggc ccgagcatga agcgcattat gccgcgtgca aaaggtcgtg
     5101 cagatcgcat cctgaagcgc accagccaca tcactgtggt tgtgtccgat cgctgagact
     5161 ctggagacta gcaatgggtc agaaagtaca tcctaatggt attcgcctgg gtattgtaaa
     5221 accatggaac tctacctggt ttgcgaacac caaagaattc gctgacaacc tggacagcga
     5281 ttttaaagta cgtcagtacc tgactaagga actggctaaa gcgtccgtat ctcgtatcgt
     5341 tatcgagcgt ccggctaaga gcatccgtgt aaccattcac actgctcgcc cgggtatcgt
     5401 tatcggtaaa aaaggtgaag acgtagaaaa actgcgtaag gtcgtagcgg acatcgctgg
     5461 cgttcctgca cagatcaaca tcgccgaagt tcgtaagcct gaactggacg caaaactggt
     5521 tgctgacagc atcacttctc agctggaacg tcgcgttatg ttccgtcgtg ctatgaagcg
     5581 tgctgtacag aacgcaatgc gtctgggcgc taaaggtatt aaagttgaag ttagcggccg
     5641 tctgggcggc gcggaaatcg cacgtaccga atggtaccgc gaaggtcgcg taccgctgca
     5701 cactctgcgt gctgacatcg actacaacac ctctgaagcg cacaccactt acggtgtaat
     5761 cggcgttaaa gtgtggatct tcaaaggcga gatcctgggt ggtatggctg ctgttgaaca
     5821 accggaaaaa ccggctgctc agcctaaaaa gcagcagcgt aaaggccgta aataaggagc
     5881 gtcgctgatg ttacaaccaa agcgtacaaa attccgtaaa atgcacaaag gccgtaaccg
     5941 cggtctggcg cagggtacgg atgttagctt cggcagcttc ggtctgaaag ctgttggccg
     6001 tggtcgtctg actgcccgtc agatcgaagc agcacgtcgt gctatgaccc gtgcagttaa
     6061 gcgtcaaggt aagatctgga tccgtgtgtt cccggacaaa ccgatcactg aaaagccgct
     6121 ggcagtgcgt atgggtaaag gtaaaggtaa cgtggagtat tgggttgcct tgattcagcc
     6181 gggtaaagtc ctgtatgaaa tggacggtgt tccggaagag ctggcccgtg aagcattcaa
     6241 gctggcagca gcgaaactgc cgattaaaac cacctttgta actaagacgg tgatgtaatg
     6301 aaagcaaaag agctgcgtga gaagagcgtt gaagagctga acaccgagct gctgaacctg
     6361 ctgcgtgagc agttcaacct gcgtatgcag gctgcaagtg gccagctgca acagtctcac
     6421 ctgttgaagc aagtgcgtcg cgatgtcgca cgcgttaaga ctttactgaa cgagaaggcg
     6481 ggtgcgtaat gaccgataaa atccgtactc tgcaaggtcg cgttgttagc gacaaaatgg
     6541 agaaatccat tgttgttgct atcgaacgtt ttgtgaaaca cccgatctac ggtaaattca
     6601 tcaagcgtac gaccaaactg cacgtacatg acgagaacaa cgaatgcggt atcggtgacg
     6661 tggttgaaat ccgcgaatgc cgtccgctgt ccaagactaa atcctggacg ctggttcgcg
     6721 ttgtagagaa agcggttctg taatacagta cactctctcg atacgaataa acggctcaga
     6781 aatgagccgt ttattttttc tacccatatc cttgaagcgg tgttataatg ccgcgccctc
     6841 gatatgggga tttttaacga cctgattttc gggtctcagt agtagttgac attagcggag
     6901 cactaaaatg atccaagaac agactatgct gaacgtcgcc gacaactccg gtgcacgtcg
     6961 cgtaatgtgt atcaaggttc tgggtggctc gcaccgtcgc tacgcaggcg taggcgacat
     7021 catcaagatc accatcaaag aagcaattcc gcgtggtaag gtcaaaaaag gtgatgtgct
     7081 gaaggcggta gtggtgcgca ccaagaaggg tgttcgtcgc ccggacggtt ctgtcattcg
     7141 cttcgatggt aatgcttgtg ttcttctgaa caacaacagc gagcagccta tcggtacgcg
     7201 tatttttggg ccggtaactc gtgagcttcg tagtgagaag ttcatgaaaa ttatctctct
     7261 ggcaccagaa gtactctaag gagcgaatca tggcagcgaa aatccgtcgt gatgacgaag
     7321 ttatcgtgtt aaccggtaaa gataaaggta aacgcggtaa agttaagaat gtcctgtctt
     7381 ccggcaaggt cattgttgaa ggtatcaacc tggttaagaa acatcagaag ccggttccgg
     7441 ccctgaacca accgggtggc atcgttgaaa aagaagccgc tattcaggtt tccaacgtag
     7501 caatcttcaa tgcggcaacc ggcaaggctg accgtgtagg ctttagattc gaagacggca
     7561 aaaaagtccg tttcttcaag tctaacagcg aaactatcaa gtaatttgga gtagtacgat
     7621 ggcgaaactg catgattact acaaagacga agtagttaaa aaactcatga ctgagtttaa
     7681 ctacaattct gtcatgcaag tccctcgggt cgagaagatc accctgaaca tgggtgttgg
     7741 tgaagcgatc gctgacaaaa aactgctgga taacgcagca gcagacctgg cagcaatctc
     7801 cggtcaaaaa ccgctgatca ccaaagcacg caaatctgtt gcaggcttca aaatccgtca
     7861 gggctatccg atcggctgta aagtaactct gcgtggcgaa cgcatgtggg agttctttga
     7921 gcgcctgatc actattgctg tacctcgtat ccgtgacttc cgtggcctgt ccgctaagtc
     7981 tttcgacggt cgtggtaact acagcatggg tgtccgtgag cagatcatct tcccagaaat
     8041 cgactacgat aaagtcgacc gcgttcgtgg tttggatatt accattacca ctactgcgaa
     8101 atctgacgaa gaaggccgcg ctctgctggc tgcctttgac ttcccgttcc gcaagtaagg
     8161 tagggttact aaatggctaa gcaatcaatg aaagcacgcg aagtaaaacg cgtagcttta
     8221 gctgataaat acttcgcgaa acgcgctgaa ctgaaagcga tcatctctga tgtgaacgct
     8281 tccgacgaag atcgttggaa cgctgttctc aagctgcaga ctctgccgcg tgattccagc
     8341 ccgtctcgtc agcgtaaccg ctgccgtcaa acaggtcgtc cgcatggttt cctgcggaag
     8401 ttcgggttga gccgtattaa ggtccgtgaa gccgctatgc gcggtgaaat cccgggtctg
     8461 aaaaaggcta gctggtaatt gtcaccaatt gaatcacggg agtaaagaca gatgagcatg
     8521 caagatccga tcgcggatat gctgacccgt atccgtaacg gtcaggccgc gaacaaagct
     8581 gcggtcacca tgccttcctc caagctgaaa gtggcaatcg ccaacgtgct gaaggaagaa
     8641 ggttttattg aaggttttaa agttgaaggc gacaccaagc ctgaactgga acttactctg
     8701 aagtatttcc agggcaaagc tgttgtagaa agcattcagc gtgtcagccg cccaggtctg
     8761 cgcatctata aacgtaaaga tgagctgccg aaagttatgg cgggtctggg tatcgcagtt
     8821 gtttctacct ctaaaggtgt tatgactgat cgtgcagcgc gccaggctgg tcttggtggc
     8881 gaaattatct gctacgtagc ctaatcggag gaaaaaatgt ctcgtgttgc taaagcaccg
     8941 gtcgttgttc ctgccggcgt tgacgtaaaa atcaacggtc aggttattac gatcaaaggt
     9001 aaaaacggcg agctgactcg tactctcaac gatgctgttg aagttaaaca tgcagataat
     9061 accctgacct tcggtccgcg tgatggttac gcagacggtt gggcacaggc tggtaccgcg
     9121 cgtgccctgc tgaactcaat ggttatcggt gttaccgaag gcttcactaa gaagctgcag
     9181 ctggttggtg taggttaccg tgcagcggtt aaaggcaatg tgattaacct gtctctgggt
     9241 ttctctcatc ctgttgacca tcagctgcct gcgggtatca ctgctgaatg tccgactcag
     9301 actgaaatcg tgctgaaagg cgctgataag caggtgatcg gccaggttgc agcggatctg
     9361 cgcgcctacc gtcgtcctga gccttataaa ggcaagggtg ttcgttacgc cgacgaagtc
     9421 gtgcgtacca aagaggctaa gaagaagtaa ggtaacacta tggataagaa atctgctcgt
     9481 atccgtcgtg cgacccgcgc acgccgcaag ctccaggagc tgggcgcaac tcgcctggtg
     9541 gtacatcgta ccccgcgtca catttacgca caggtaattg caccgaacgg ttctgaagtt
     9601 ctggtagctg cttctactgt agaaaaagct atcgctgaac aactgaagta caccggtaac
     9661 aaagacgcgg ctgcagctgt gggtaaagct gtcgctgaac gcgctctgga aaaaggcatc
     9721 aaagatgtat cctttgaccg ttccgggttc caatatcatg gtcgtgtcca ggcactggca
     9781 gatgctgccc gtgaagctgg ccttcagttc taaggtagag gtgtaagatg gctcacatcg
     9841 aaaaacaagc tggcgaactg caggaaaagc tgatcgcggt aaaccgcgta tctaaaaccg
     9901 ttaaaggtgg tcgtattttc tccttcacag ctctgactgt agttggcgat ggtaacggtc
     9961 gcgttggttt tggttacggt aaagcgcgtg aagttccagc agcgatccag aaagcgatgg
    10021 aaaaagcccg tcgcaatatg attaacgtcg cgctgaataa cggcactctg caacaccctg
    10081 ttaaaggtgt tcacacgggt tctcgcgtat tcatgcagcc ggcttccgaa ggtaccggta
    10141 tcatcgccgg tggtgcaatg cgcgccgttc tggaagtcgc tggggttcat aacgttctgg
    10201 ctaaagcata tggttccacc aacccgatca acgtggttcg tgcaactatt gatggcctgg
    10261 aaaatatgaa ttctccagaa atggtcgctg ccaagcgtgg taaatccgtt gaagaaattc
    10321 tggggaaata aaccatggca aagactatta aaattactca aacccgcagt gcaatcggtc
    10381 gtctgccgaa acacaaggca acgctgcttg gcctgggtct gcgtcgtatt ggtcacaccg
    10441 tagagcgcga ggatactcct gctattcgcg gtatgatcaa cgcggtttcc ttcatggtta
    10501 aagttgagga gtaagagatg cgtttaaata ctctgtctcc ggccgaaggc tccaaaaagg
    10561 cgggtaaacg cctgggtcgt ggtatcggtt ctggcctcgg taaaaccggt ggtcgtggtc
    10621 acaaaggtca gaaatctcgt tctggcggtg gcgtacgtcg cggtttcgag ggtggtcaga
    10681 tgcctctgta ccgtcgtctg ccgaaattcg gcttcacttc tcgtaaagca gcgattacag
    10741 ccgaagttcg tctgtctgac ctggctaaag tagaaggcgg tgttgtagac ctgaacacgc
    10801 tgaaagcggc taacattatc ggtatccaga tcgagttcgc gaaagtgatc ctggctggcg
    10861 aagtaacgac tccggtaact gttcgtggcc tgcgtgttac taaaggcgct cgtgctgcta
    10921 tcgaagctgc tggcggtaaa atcgaggaat aagtagcaga tggctaaaca accgggatta
    10981 gattttcaaa gtgccaaagg tggcttaggc gagctgaaac gcagactgct gtttgttatc
    11041 ggtgcgctga ttgtgttccg tattggctct tttattccga tccctggtat tgatgccgct
    11101 gtacttgcca aactgcttga gcaacagcga ggcaccatca ttgagatgtt taacatgttc
    11161 tctggtggtg ctctcagccg tgcttctatc tttgctctgg ggatcatgcc gtatatttcg
    11221 gcgtcgatca ttatccagct gctgacggta gttcacccaa cgttggcaga aattaagaaa
    11281 gaaggggagt ctggtcgtcg taagatcagc cagtacaccc gttacggtac tctggtgctg
    11341 gcaatattcc agtcgatcgg tattgctacc ggtctgccga atatgcctgg tatgcaaggc
    11401 ctggtgatta acccaggctt tgcattctac ttcaccgctg ttgtaagtct ggtcacagga
    11461 acgatgttcc tgatgtggtt gggcgaacag attactgaac gaggtatcgg caacggtatt
    11521 tcaatcatta tcttcgccgg tattgtcgcg ggactcccgc cagccattgc ccatactatc
    11581 gagcaagcgc gtcaaggcga cctgcacttc ctcgtgttgc tgttggttgc agtattagta
    11641 tttgcagtga cgttctttgt tgtatttgtt gagcgtggtc aacgccgcat tgtggtaaac
    11701 tacgcgaaac gtcagcaagg tcgtcgtgtc tatgctgcac agagcacaca tttaccgctg
    11761 aaagtgaata tggcgggggt aatcccggca atcttcgctt ccagtattat tctgttcccg
    11821 gcgaccatcg cgtcatggtt cgggggcggt actggttgga actggctgac aacaatttcg
    11881 ctgtatttgc agcctgggca accgctttat gtgttactct atgcgtctgc aatcatcttc
    11941 ttctgtttct tctacacggc gttggttttc aacccgcgtg aaacagcaga taacctgaag
    12001 aagtccggtg catttgtacc aggaattcgt ccgggagagc aaacggcgaa gtatatcgat
    12061 aaagtaatga cccgcctgac cctggttggt gcgctgtata ttacctttat ctgcctgatc
    12121 ccggagttca tgcgtgatgc aatgaaagta ccgttctact tcggtgggac ctcactgctt
    12181 atcgttgttg tcgtgattat ggactttatg gctcaagtgc aaactctgat gatgtccagt
    12241 cagtatgagt ctgcattgaa gaaggcgaac ctgaaaggct acggccgata attggtcgcc
    12301 cgagaagtta cggagagtaa aaatgaaagt tcgtgcttcc gtcaagaaat tatgccgtaa
    12361 ctgcaaaatc gttaagcgtg atggtgtcat ccgtgtgatt tgcagtgccg agccgaagca
    12421 taaacagcgc caaggctgat tttttcgcat atttttcttg caaagttggg ttgagctggc
    12481 tagattagcc agccaatctt ttgtatgtct gtgcgtttcc atttgagtat cctgaaaacg
    12541 ggcttttcag catggaacgt acatattaaa tagtaggagt gcatagtggc ccgtatagca
    12601 ggcattaaca ttcctgatca taagcatgcc gtaatcgcat taacttcgat ttatggcgtc
    12661 ggcaagaccc gttctaaagc catcctggct gcagcgggta tcgctgaaga tgttaagatc
    12721 agtgagctgt ctgaaggaca aatcgacacg ctgcgtgacg aagttgccaa atttgtcgtt
    12781 gaaggtgatc tgcgccgtga aatcagcatg agcatcaagc gcctgatgga tcttggttgc
    12841 tatcgcggtt tgcgtcatcg tcgtggtctc ccggttcgcg gtcagcgtac caagaccaac
    12901 gcacgtaccc gtaagggtcc gcgcaaaccg atcaagaaat aatcggggtg attgaataat
    12961 ggcaaaggca ccaattcgtg cacgtaaacg tgtaagaaaa caagtctctg acggcgtggc
    13021 tcatatccat gcttctttca acaacaccat cgtgactatc actgatcgtc agggtaacgc
    13081 gttgggttgg gcaacagccg gtggttccgg tttccgtggt tctcgcaaat ccactccgtt
    13141 tgcagctcag gttgcagcag agcgttgcgc tgacgccgtg aaagaatacg gcatcaagaa
    13201 tctggaagtt atggttaaag gtccgggtcc aggccgcgaa tctactattc gtgctctgaa
    13261 cgccgcaggt ttccgcatca ctaacattac tgatgtgact ccgatccctc ataacggttg
    13321 tcgtccgccg aaaaaacgtc gcgtataacg cctcgttttc caggtttgtt ggagaaagaa
    13381 aatggcaaga tatttgggtc ctaagctcaa gctgagccgt cgtgagggca ccgacttatt
    13441 ccttaagtct ggcgttcgcg cgatcgatac caagtgtaaa attgaacaag ctcctggcca
    13501 gcacggtgcg cgtaaaccgc gtctgtctga ctatggtgtg cagttgcgtg aaaagcaaaa
    13561 agttcgccgt atctatggtg tgctggagcg tcagttccgt aactactaca aagaagcagc
    13621 acgtctgaaa ggcaacaccg gtgaaaacct gttggctctg ctggaaggtc gtctggacaa
    13681 cgttgtatac cgtatgggct tcggtgccac tcgtgcagaa gcacgtcagc tggttagcca
    13741 taaagcaatt atggtaaacg gtcgtgttgt taacatcgct tcttatcagg ttagtccgaa
    13801 tgacgttgta agcattcgtg agaaagcgaa gaagcagtct cgcgtgaaag ccgctctgga
    13861 gctggctgag cagcgtgaaa agccaacctg gctggaagtt gatgctggca agatggaagg
    13921 tacgtttaag cgtaagccgg agcgttctga tctgtctgcg gacattaacg aacacctgat
    13981 cgtcgagctt tactccaagt aaagcttagt accaaagaga ggacacaatg cagggttctg
    14041 tgacagagtt tctaaaaccg cgcctggttg atatcgagca agtgagttcg acgcacgcca
    14101 aggtgaccct tgagccttta gagcgtggct ttggccatac tctgggtaac gcactgcgcc
    14161 gtattctgct ctcatcgatg ccgggttgcg cggtgaccga ggttgagatt gatggtgtac
    14221 tacatgagta cagcaccaaa gaaggcgttc aggaagatat cctggaaatc ctgctcaacc
    14281 tgaaagggct ggcggtgaga gttcagggca aagatgaagt tattcttacc ttgaataaat
    14341 ctggcattgg ccctgtgact gcagccgata tcacccacga cggtgatgtc gaaatcgtca
    14401 agccgcagca cgtgatctgc cacctgaccg atgagaacgc gtctattagc atgcgtatca
    14461 aagttcagcg cggtcgtggt tatgtgccgg cttctacccg aattcattcg gaagaagatg
    14521 agcgcccaat cggccgtctg ctggtcgacg catgctacag ccctgtggag cgtattgcct
    14581 acaatgttga agcagcgcgt gtagaacagc gtaccgacct ggacaagctg gtcatcgaaa
    14641 tggaaaccaa cggcacaatc gatcctgaag aggcgattcg tcgtgcggca accattctgg
    14701 ctgaacaact ggaagctttc gttgacttac gtgatgtacg tcagcctgaa gtgaaagaag
    14761 agaaaccaga gttcgatccg atcctgctgc gccctgttga cgatctggaa ttgactgtcc
    14821 gctctgctaa ctgccttaaa gcagaagcta tccactatat cggtgatctg gtacaacgta
    14881 ccgaggttga gctccttaaa acgcctaacc ttggtaaaaa atctcttact gagattaaag
    14941 acgtgctggc ttcccgtgga ctgtctctgg gcatgcgcct ggaaaactgg ccaccggcaa
    15001 gcatcgctga cgagtaaccg gatcacaggt taaggtttta ctgagaagga taaggtcatg
    15061 cgccatcgta agagtggtcg tcaactgaac cgcaacagca gccatcgcca ggctatgttc
    15121 cgcaatatgg caggttcact ggttcgtcat gaaatcatca agacgactct gcctaaagcg
    15181 aaagagctgc gccgcgtagt tgagccgctg attactcttg ccaagactga tagcgttgct
    15241 aatcgtcgtc tggcattcgc ccgtactcgt gataacgaga tcgtggcaaa actgtttaac
    15301 gaactgggcc cgcgtttcgc gagccgtgcc ggtggttaca ctcgtattct gaagtgtggc
    15361 ttccgtgcag gcgacaacgc gccgatggct tacatcgagc tggttgatcg ttcagagaaa
    15421 gcagaagctg ctgcagagta atctgaagca acgtaaaaaa acccgccccg gcgggttttt
    15481 ttatacccgt agcatcccca cttatctaca atagctgtac tctttttgtt catcccctgg
    15541 agtatttatg tggttacttg accagtgggc agagcgtcat atagcagaag cgcaagcgaa
    15601 aggtgagttt gataacctgg caggtagcgg cgaaccattg atactggatg atgattctca
    15661 tgtgccaccg gaattacgtg cggggtatcg cttgctgaag aatgccggtt gcttaccgcc
    15721 agaacttgag cagcggaggg aagcaattca gcttctggat atcctgagag gtatccgtca
    15781 cgatgatccg caatatcaag aggttagccg tcgattgtca ttactggaat tgaagctgcg
    15841 acaagctgga ttgagtaccg attttttacg cggcgattat gctgacaagt tgttggacaa
    15901 aatcaacgat aactagtgga gtatgtatgt atcgcattgg tgagctggca aaaatggcgg
    15961 aagtaacacc tgacacgatt cgttattacg aaaaacagca gatgatggag catgaagtgc
    16021 gtactgaagg cgggtttcgc ctgtataccg aaagcgatct ccagcgattg aaatttatcc
    16081 gccatgccag acaactaggt ttcagtctgg agtcgatccg cgagttgctg tcgatccgca
    16141 tcgatcctga acaccatacc tgtcaggagt caaaaggcat tgtgcaggaa agattgcagg
    16201 aagtcgaagc acggatagcc gagttgcaga gtatgcagcg ttccttgcaa cgccttaacg
    16261 atgcctgttg tgggaccgcc catagcagtg tttattgttc gattcttgaa gctcttgaac
    16321 aaggggcgag tggcgttaag agtggctgtt gattttttgc actgacagga ttacactcgc
    16381 gccgtaaaat aaccaactgg agtttttatg agccgatatc agcatactaa agggcagata
    16441 aaggataatg cgatagaagc attactacat gatcccttat tccgacagcg agtagagaaa
    16501 aataagaaag ggaaaggcag ttacatgcga aaaggaaaac atggcaatcg ggggaactgg
    16561 gaggccagtg gcaagaaagt aaatcacttt tttaccactg gtcttctgct ttcaggcgct
    16621 tgttaagagc ggttattctg ctctttcagc aaatcacgaa tttctgccag taatacttct
    16681 tctttagttg gtgcaggtgc ggcagctggt tcttcttttt tccgattcag tttgttgatt
    16741 agcttaatcg ccataaagat ggcaaaggcc acaatcagaa aatcaaagac gttttgaatg
    16801 aagacaccgt aatgcatcac aacagcaggg atatccccct gcgcatcgcg tagcgtgaca
    16861 gcaaactgtt taaaatcgat cccgccaatt aataagccca gaggaggcat gatgatatcg
    16921 gcaaccagtg aagagacaat cttcccgaat gccgcaccga taatgacacc caccgccaaa
    16981 tccaccacgt tcccgcgcat cgcaaattcg cgaaattctt taataatgct catgttattc
    17041 tccctatgaa gccgacaacc ataagtctaa caaatgttaa gccattttcc tgccaggaat
    17101 gaatttaatt aatccttgat aatcaaaggg aaataataag gcgtcattag acgccttatt
    17161 aattacaaga agaaagggct tggttggaag agtctttcga cgtcggtaat aaacttttta
    17221 tctgtgagga acataattac gtgatcgcct tgctcaatgc gcagattgtc attggcaatc
    17281 atcacgtcgt ttccacgtac caccgctcca ataatcgttc ctggcggtag cttgatttcg
    17341 tcaatgactc tgccgacaac gcgtgaggtg ctttcatcac cgtgagcaac ggcttcaata
    17401 gcttctgcta cgccgcggcg caatgaggaa acaccaacaa tatctgcttt tcgcacatgg
    17461 ctaagcaacg cagaaatagt tgcttgttgt ggtgaaatcg caatatcgat cacactcccc
    17521 tgaaccagat ccacataagc gcgacgctgg atcaatacca tcaccttttt cgcacccata
    17581 cgtttggcaa gcatggcgga catgatattg gcctcgtcat cgttggtgac ggcaataaac
    17641 agatcaactt gatcgatatg ttcttcggcc agcagttctt gatccgacgc atcaccaaaa
    17701 aagacgatcg tattctgcaa cttttccgcc agttcggcag cgcgctgctg attacgttcg
    17761 atgagtttaa cgctgtaatc tttttccaga cgacgcgcca gccctgcacc gatattaccg
    17821 ccaccaacca gcatgatccg cttatacggt ttttccagtc gctgtaattc actcatcacc
    17881 gcacggatat gctgtgaagc ggcaataaag aacacttcat caccagcttc cacaatggtc
    17941 gaaccttgtg gacgaatggg gcgatcgtgg cggaaaattg ctgccacacg agtatcgata
    18001 tgtggcatat gttcgcgcat ggtcgaaagt gcgttaccaa tcagcgggcc gccataatag
    18061 gctttaacca cagcgaggct gactttaccc tcagcgaagt tcaccacctg caatgcgccg
    18121 ggatactcaa tcagtcggta aatattatcg ataaccaact gctctggtgc gatcagatga
    18181 tcaatcggta cagcatctga atgaaatagc ttatcggcat cgcgcacgta gtctggtgag
    18241 cggatgcgag cgatgcgatt aggggtgttg aaaagtgagt aggctacctg gcaggcaacc
    18301 atattggttt catctgaact ggttacagca accagcatat cggcgtcgtc ggcacctgcc
    18361 tcccgcaata cgcgtggatg agagccatgc ccctgcacga ctcgcaggtc aaatttatcc
    18421 tgcaaggtcc gcagacgctc accgttggta tcgacaacag tgatatcgtt gttctcgcca
    18481 accaggtttt ccgccagtgt gccgccaacc tggccggcac ccagaatgat aattttcatc
    18541 agtcgcgacc cgttatctca tcactttttg attagcttag cgtaaaagaa gccgtcgccc
    18601 tcttcggtac caggcagatt ttgtttaccc ggttgctctg gtgttcctgt ttcgcaaagt
    18661 tcggcatcag cggtacgttg caaaaaggct ttaatctgca ggctattctc ttccggtaac
    18721 accgaacagg tggcatagac cagggttcca ccggatttta aatgcggcca aatggcgtcg
    18781 agaatttcag actgcaattg cgcgagttcc gggatatcgc gatcgcgacg taaccattta
    18841 atatccggat ggcgacgaat cacaccggtt gctgaacaag gcgcatctaa taaaatgcga
    18901 tcaaactgtt gctcgccaca ccattgggaa gggtagcggc catcaccttg tttcacggtc
    18961 gctttcatac caaggcgttt taaattgtcg taaacgcgag agaggcgctg ttcgtcaata
    19021 tcaaccgcaa caacctgcgc ttctggtgcc acctcaagga tatgcgttgt tttaccgccg
    19081 ggggcggcac aaagatccag aatgtgttca ccgttttgtg gcgcaagcca ggtcatgcaa
    19141 ccttgtgctg atgcatcctg aacggtaacc catccgtctt caaaaccagg tagcgcatga
    19201 acaggtgcag gtgtttccag ccgtacagca tcagggtaat ccgcatgcgg gaaacctttc
    19261 attcctgctt catccaacaa tgcaagccag ctgtcgcggg aatgatgcgt acgattaacg
    19321 cgcagccaca ttggcggacg ctggttatta gcttcgacga tggattgcca ctgctctgga
    19381 tacgcttttt gcagacgctt cagcaaccag gaaggatgca gataacgtgc atcactggca
    19441 taaaacccgg ctaataactc ttcttgctga cgctggaact ggcgtaatac accgttaatc
    19501 agccccttaa gttgcgggcg cttaattgcg acagcgcctt caaccgtttc agccagcgca
    19561 gcatgaggtg gaatgcgggt ataaagcagt tgatacacac caaccataat caggtaatgc
    19621 acggtccgct gtttgccggt catcggacgg gccattaact tattaatcag ccagtctaac
    19681 tgtgaaagcg tacgcagtac gccaaagcac aactcttgaa gaagtgcttt gtctttatcg
    19741 gaaacttttt gctggagcgg tggcagaatg ttgcttaatg attgcccttg ctcgacgatt
    19801 tgttcaacgg cctgtgccgc catgctacgt aaattacgtt gttttttcat aagtataaaa
    19861 atgcccggta agaccgggct tagaagagtg gactatcaga ccagacggtt gcccggaaca
    19921 aaccattccc gacgagaatt caggaggtct tgcgcgctca tcgctttctt acccgcaggt
    19981 tgtaacgaga gcaggttcag gatgccatca ccagtcgcaa cctgaatgcc ttgtttattg
    20041 gcttcaagga tcgttcctgg tgcagcgttg gttgccgtat caatgaccga cgctttccag
    20101 actttaaccg gttgtccttc aatttccagc cagctcattg gccatgggtt gaaagcgcga
    20161 atgcagcgtt caagctgcgc tgccgaaagc gaccagtcaa tacgcgcttc ttctttactc
    20221 aacttctcgg cgtaagtgac aagagtttcg tcctgaactt ctggtttcgc cgtgccgtct
    20281 gctagttgtt tcaacgtggt gataagccct tgtgggccaa gctctgccag cttgtcgtac
    20341 agcgtaccac tggtatcttc tgcagtaatc gggcaggaga gcttataaag catgtcaccg
    20401 gtgtctaaac caacatccat ttgcataatg gtcacaccag tttctgcatc acccgcccat
    20461 agtgagcgtt ggattggtgc agcaccgcgc cagcgtggca gcagtgaacc atgaacgttg
    20521 atacagccaa gacgcggcat ctccagcact gctttaggca gaattaagcc ataggcgacg
    20581 acgaccataa catcagcctg cagatcggcg accagttgct ggctttcttg tggacgcagg
    20641 gaaacaggtt gaaaaacggg cagacctttt tcctcagcca gaactttaac cgggctgggc
    20701 atcagttttt taccgcgtcc tgccggtcgg tctggctggg tgaacacgcc aacgacgtta
    20761 tgaccagaag acaacagcgc gtcgagatga cgcgctgcaa agtcaggtgt acccgcaaaa
    20821 ataatacgta gtgattctga cacgttagtt cttatcctta agcccgggct ttcagacgat
    20881 ctagtttttc aactttctga cgaatacgtt gttgtttcag cggtgacaga taatccataa
    20941 acagtttgcc gaccaggtga tccatctcat gctgaataca gatggctaac agcccctctg
    21001 cttccagttc aaatggttta ccgtcgcggt caagggcgcg aattttaact ttctctgcgc
    21061 gcggcactaa agcacgttgt tcagggatcg acaggcaacc ttcttcaatg cctgtttcgc
    21121 cgcttttttc taaaagctcc ggattgatta acaccagccg ttcgtcacgg ttttccgaaa
    21181 catcaataac aatgatacgt tgatggatat caacctgggt tgccgccagg ccaatacctt
    21241 cttctgcgta catcgtctcg aacatatcat cgacgatacg ctgtatttct gcattcactt
    21301 cttctaccgg tttagcaact ttgcgaagcc gctcgtccgg aatatgtaac acttgcaaaa
    21361 ctgacataaa tctccagaga tgtgttcagg agttagaaag attatttctt ctattctaga
    21421 caaatccccc tctgattgac agcatcactg accaatcgca aagattgcta aggctgctta
    21481 tggcagggag ataaggatgg tcgatacaga aatttggctg cgtttaatga gtatcagcag
    21541 cttgtacggc gatgatatgg tccgtatagc tcactggctg gcaaaacagt cgcaaattga
    21601 tgcggttgga ttgcagcaaa cagggcttac attgcggcag gcacaacgct ttctttcatt
    21661 tccgcgaaag agtatcgaaa gctcactttg ttggttggag caacccaacc atcatttaat
    21721 ccctgcggac agcgaatttt atcctcctca acttctggcg acgacagatt accccggcgc
    21781 actgtttgtt gaaggagaac tgcacgcgct gcattcattt cagcttgccg tagtggggag
    21841 tcgggcgcat tcatggtatg gcgagcgatg gggacggtta ttttgcgaaa ctctggcgac
    21901 gcgtggagtg acaattacga gtggactggc gcgtggaatc gatggtgtgg cgcataaagc
    21961 ggccttacag gtaaatggcg tcagcattgc tgtattgggg aatggactta ataccattca
    22021 tccccgccgc catgcccgac tggctgccag tctgcttgaa catggcggag ctctcgtctc
    22081 ggaatttccc ctcgatgttc caccccttgc ttacaatttc ccacgaagaa atcgcattat
    22141 cagtggtcta agtaaaggtg tactggtggt ggaagcggct ttgcgcagtg gttcgctggt
    22201 gacagcacgt tgtgcgcttg agcaggggcg tgaagttttt gccttgccag gtccaatagg
    22261 gaatccggga agcgaagggc ctcactggtt aataaaacaa ggtgcgattc ttgtgacgga
    22321 accggaagaa attctggaaa acttgcaatt tggattgcac tggttgccag acgcccctga
    22381 aaattcattt tattcaccag atcagcaaga cgtggcattg ccatttcctg agctcctggc
    22441 taacgtagga gatgaggtaa cacctgttga cgtcgtcgct gaacgtgccg gccaacctgt
    22501 gccagaggta gttactcaac tactcgaact ggagttagca ggatggatcg cagctgtacc
    22561 cggcggctat gtccgattga ggagggcatg ccatgttcga cgtactaatg tatttgtttg
    22621 aaacctatat tcacacagaa gctgagttgc gtgtggatca agacaaactt gaacaggatc
    22681 ttaccgacgc aggatttgat cgagaagata tctacaatgc cctgctttgg ctggaaaagc
    22741 ttgcggatta tcaggaagga ctggcagaac cgatgcaact ggcctctgat ccactctcca
    22801 tgcgtattta tacccctgag gagtgtgaaa gactggatgc cagctgtcgt ggttttctgc
    22861 ttttccttga gcaaattcag gtgcttaacc ttgaaacccg tgaaatggtg atagagcgag
    22921 tgctcgcgct ggataccgca gagttcgacc tggaagacct gaaatgggta atcctgatgg
    22981 tgttgttcaa tattccgggc tgcgaaaatg cgtaccagca aatggaagaa ttactctttg
    23041 aagtgaatga aggtatgctg cattaatcat ttctttaatt cagcataagt tgttatggcg
    23101 aaatcagcac tgttcacggt gcgtaataat gagtcctgcc caaagtgcgg ggctgaactg
    23161 gttattcgat ccgggaaaca cggtccgttt cttggatgct cacagtatcc ggcgtgtgac
    23221 tacgtccgtc ctctgaaatc ttcagcggat ggacatattg tcaaagttct ggaggggcag
    23281 gtttgccttg catgtggcgc aaatctggta ttacgccagg gacgctttgg tatgtttatt
    23341 ggttgcagta actaccctga atgcgaacat accgaactta tcgataaacc ggacgaaaca
    23401 gcaattacat gcccccaatg ccggacgggc catctggtcc agcgccgctc ccgttatggc
    23461 aaaacatttc actcctgtga tcgctacccg gagtgtcaat ttgccattaa cttcaaaccc
    23521 atagctggag aatgccctga gtgtcattat ccactactca tcgaaaagaa aaccgcgcag
    23581 ggtgtaaaac acttttgtgc cagtaaacaa tgtggaaagc cggtttcggc ggaataataa
    23641 cgtgaataat aacctgcaag gagacgctat cgcagctgcg atagatgtcc tcaatgaaga
    23701 acgtgtcatc gcctatccaa cggaagccgt tttcggtgtt gggtgcgatc ctgatagcga
    23761 aacagcagtg atgagactgt tggagttaaa acagcgtccg gttgataagg ggctgatttt
    23821 aatcgcggca aattacgagc agcttaaacc ctatattgat gacaccatgc tgactgatgc
    23881 gcagcgtgaa accatttttt cccgctggcc aggtcctgtc acctttgtct ttcccgcgcc
    23941 tgcgacaaca ccgcgctggt tgacgggccg ctttgattcg cttgctgtac gagtcaccga
    24001 ccatccgttg gtggttgctt tgtgccaggc ttatggtaaa ccgctggttt ctaccagtgc
    24061 caacttgagt ggattgccac cttgtcgaac agtagacgaa gttcgcgcac aatttggcgc
    24121 ggcgttcccg gttgtgcctg gtgaaacggg ggggcgttta aatccttcag aaatccgcga
    24181 tgccctgacg ggtgaactgt ttcgacaggg gtaacataat ggaaacctat gctgtttttg
    24241 gtaatccgat agcccacagc aaatcgccat tcattcatca gcaatttgct cagcaactga
    24301 atattgaaca tccctatggg cgcgtgttgg cacccatcaa tgatttcatc aacacactga
    24361 acgctttctt tcgtgctggt ggtaaaggtg cgaatgtgac ggtgcctttt aaagaagagg
    24421 cttttgccag agcggatgag cttactgaac gggcagcgtt ggctggtgct gttaataccc
    24481 tcaagcggtt agaagatgga cgcctgctgg gtgacaatac cgatggtata ggtttgttaa
    24541 gcgatctgga acgtctgtct tttatccgtc ctggtttacg tattctgctc atcggcgctg
    24601 gtggagcatc tcgcggcgta ctactgccac ttctttccct ggactgtgcg gtgacaatca
    24661 ctaatcggac ggtatcccgc gcggaagagt tggctaaatt gtttgcgcac actggcagta
    24721 ttcaggcgtt gggtatggac gaactggaag gtcatgagtt tgatctcatt attaatgcaa
    24781 catccagtgg catcagtggt gatattccgg cgatcccgtc atcgctcatt catccaggca
    24841 tttattgcta tgacatgttc tatcagaaag gaaaaactcc ttttctggca tggtgtgagc
    24901 agcgaggctc aaagcgtaat gctgatggtt taggaatgct ggtggcacag gcggctcatg
    24961 cctttcttct ctggcacggt gttctgcctg acgtagaacc agttataaag caattgcagg
    25021 aggaattgtc cgcgtgaatc aggccatcca gtttccggac agagaagagt gggacgagaa
    25081 taaaaaatgt gtatgttttc ccgctctcgt gaatggcatg caactgacat gtgcgatctc
    25141 tggcgagagt ctggcgtatc gttttactgg agatacgcca gaacagtggt tagcgagttt
    25201 tcgtcagcat cgctgggatc tggaagaaga agcggaaaac ttaattcagg aacaaagtga
    25261 agatgatcaa ggctgggtct ggttaccctg atccagatat tcgtccttcc atttcacgta
    25321 attattcgcg gaatagcgta acccagcctt ctcttcatca cttaacgggc ggatctgttt
    25381 gacggggcta ccgagataca gatatccgct ctccagccgt ttattttgtg ggaccagact
    25441 acccgcacca atcatcacat catcttctac tattgcgcca tcaagtaaaa ttgagcccat
    25501 cccaaccaaa actcgattgc caatggtgca gccgtggagc atcaccttgt gaccaacagt
    25561 gacatcttcg ccaatggtta atgggttgcc atctgggttg tacgaggatt tatgagtgac
    25621 atgcaacata ctgccatcct ggatattggt gcgtgctccg atctgtacat aatgtacatc
    25681 tccacgaatc acaacgagcg gccagatccc cacatcatca gccagacgaa cgtcaccaat
    25741 cacgacactg ctatcgtcga tcattacgcg ctgaccgatt tgtggaaaaa gatcgcggta
    25801 tgggcgtaaa acatcagaca tacttacctc agcaataaat gatttactaa tgactttggg
    25861 ggcattattg gccttgtgca agtcttttag tatgcaaaaa agcaccgttt tgtgtgcgaa
    25921 tgcagcaaaa agggtgaaaa aacaacaaac agaaaaaaag atcaaaaaag tacttgtgca
    25981 aaaaattggg atccctataa tgcgcctccg ttgagacgac aacgtgaaac acttcacaag
    26041 atggtcgtaa caacgaagag aaaaaatcct gaaattaagg gttgactctg aaagaggaaa
    26101 gcgtaatata cgccacctcg cgacagtgag ctgaaag
//
LOCUS       DAZKHF020000039        25516 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2201S31241
            SAMN43267800-rid25106413.denovo.039, whole genome shotgun sequence.
ACCESSION   DAZKHF020000039 DAZKHF020000000
VERSION     DAZKHF020000039.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267800
            Sequence Read Archive: SRR30307263
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 25516)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25516)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 25516)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350581.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350581.2
            Genome Coverage       :: 151x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:21:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,652
            CDSs (total)                      :: 5,557
            Genes (coding)                    :: 5,320
            CDSs (with protein)               :: 5,320
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 237
            CDSs (without protein)            :: 237
            Pseudo Genes (ambiguous residues) :: 0 of 237
            Pseudo Genes (frameshifted)       :: 102 of 237
            Pseudo Genes (incomplete)         :: 130 of 237
            Pseudo Genes (internal stop)      :: 52 of 237
            Pseudo Genes (multiple problems)  :: 41 of 237
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25516
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267800-rid25106413.denovo.039"
                     /serotype="O 26:H11"
                     /isolate="2201S31241"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-01"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            complement(<1..116)
                     /locus_tag="ACHOSC_RS06660"
                     /old_locus_tag="pgaptmp_001332"
     CDS             complement(<1..116)
                     /locus_tag="ACHOSC_RS06660"
                     /old_locus_tag="pgaptmp_001332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PerC family transcriptional regulator"
                     /protein_id="HGU4547308.1"
                     /translation="MVHDRIAEELEAKGFYRRAAARWGEVMLLVETDKERHQV"
     gene            complement(127..537)
                     /locus_tag="ACHOSC_RS06665"
                     /old_locus_tag="pgaptmp_001333"
     CDS             complement(127..537)
                     /locus_tag="ACHOSC_RS06665"
                     /old_locus_tag="pgaptmp_001333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HGU4547309.1"
                     /translation="MTAQIAAYGRLVDDPQVKQTSKGTPMTLARMAVSLPCSQAQDGQ
                     ATLWLSVIAFGKQADFLAKHQKGDVASVSGTMQVSQWTGQNGETRQGYQVIADSVISA
                     RAALPGGNRRKTTGTQGNQPPAGGDDPYGDGIPF"
     gene            complement(534..812)
                     /locus_tag="ACHOSC_RS06670"
                     /old_locus_tag="pgaptmp_001334"
     CDS             complement(534..812)
                     /locus_tag="ACHOSC_RS06670"
                     /old_locus_tag="pgaptmp_001334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707047.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4547310.1"
                     /translation="MHTSGKFNKSLKKRRDRTEPKYRALDMTEHALKVAIRTIDRHAG
                     EGYAKAHPELISAFMTTTAANFATLTEREIAEAEQVTTINVKTGEVES"
     gene            complement(1101..2855)
                     /locus_tag="ACHOSC_RS06675"
                     /old_locus_tag="pgaptmp_001335"
     CDS             complement(1101..2855)
                     /locus_tag="ACHOSC_RS06675"
                     /old_locus_tag="pgaptmp_001335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707046.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5906 domain-containing protein"
                     /protein_id="HGU4547311.1"
                     /translation="MKLAPNVKQQSRGIKHKETEVIIFAGSDAWSHAKQWQEHDARMA
                     GDNEPPVWLGEQQLSELDKLQIVPEGRKSVRIFRAGYLAPVMIKAIGQKLAAAGVQDA
                     NFYPDGMHGQKVENWREYLARERQNLSDGLVIELPVKQKAQLSQMADSERAQLLADRF
                     DGVCVHPESEIVHVWRGGVWCPVSTMELSREMVAIYSEHRATFSKRVINNAVEALKVI
                     AEPMGEPSGDLLPFANGALDLKTGEFSPHTPENWITTHNGIEYTPPAPGENIRDNAPN
                     FHKWLEHAAGKDPRKMMRICAALYMIMANRYDWQMFIEATGDGGSGKSTFTHIASLLA
                     GKQNTVSAEMTSLDDAGGRAQVVGSRLIVLADQPKYTGEGTGIKKITGGDPVEINPKY
                     EKRFTAVIRAVVLATNNNPMIFTERAGGVARRRVIFRFDNIVSEAEKDRELPEKIAAE
                     IPVIIRRLLANFTDPEKARALLLEQRDGDEALAIKQQTDPVIEFCQFLNFLEEARGLM
                     MGGGGDSVKYTTRNSLYRVYLAFMAYAGRSKPLNVNDFGKAMKPAAKVYGHEYITRKV
                     KGVTQTNAITTDECDAFL"
     gene            complement(2852..3151)
                     /locus_tag="ACHOSC_RS06680"
                     /old_locus_tag="pgaptmp_001336"
     CDS             complement(2852..3151)
                     /locus_tag="ACHOSC_RS06680"
                     /old_locus_tag="pgaptmp_001336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4222 domain-containing protein"
                     /protein_id="HGU4547312.1"
                     /translation="MKLKYSGLTASGNTHPKFTRGDIYRDQYGGTVMIKGVEERRVTY
                     RREGYEYDCVMPVYQFRRDFSLVQAAPRSKPTSRKKARANIQEIKKMLNVFRGKK"
     gene            complement(3157..3390)
                     /locus_tag="ACHOSC_RS06685"
                     /old_locus_tag="pgaptmp_001337"
     CDS             complement(3157..3390)
                     /locus_tag="ACHOSC_RS06685"
                     /old_locus_tag="pgaptmp_001337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4547313.1"
                     /translation="MRDIYHETIYRAFLALSHSENMLEILRIWLETLGDNERDKQKSR
                     IATALITLLEPVIMELQEIDLLHDRYKEQHTGE"
     gene            complement(3383..3616)
                     /locus_tag="ACHOSC_RS06690"
                     /old_locus_tag="pgaptmp_001338"
     CDS             complement(3383..3616)
                     /locus_tag="ACHOSC_RS06690"
                     /old_locus_tag="pgaptmp_001338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001373432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4547314.1"
                     /translation="MRDDRFNSLKQEFSGVPDDAADALSSMPELIRAAFFLLSTREYK
                     STGLDVLNIAADYAEYVAEAHYRRKFPEDVSHA"
     gene            complement(3609..3848)
                     /locus_tag="ACHOSC_RS06695"
                     /old_locus_tag="pgaptmp_001339"
     CDS             complement(3609..3848)
                     /locus_tag="ACHOSC_RS06695"
                     /old_locus_tag="pgaptmp_001339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001488088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4547315.1"
                     /translation="MCNNTRPDAAAEAIITLMHALIDISVIADRAHKHDTSETEYAGA
                     FVPHSLAVMQLSADMALNEAKAILIADCENGGGYA"
     gene            complement(3838..4044)
                     /locus_tag="ACHOSC_RS06700"
                     /old_locus_tag="pgaptmp_001340"
     CDS             complement(3838..4044)
                     /locus_tag="ACHOSC_RS06700"
                     /old_locus_tag="pgaptmp_001340"
                     /inference="COORDINATES: protein motif:HMM:NF033153.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="host cell division inhibitor Icd-like protein"
                     /protein_id="HGU4547316.1"
                     /translation="MRNHTTHPQGRDSHDLNKYIWRFIALSTAQPRVITIEATSEQEA
                     RQQSPAGCVMVFAARIRQEVCHVQ"
     gene            4244..4423
                     /locus_tag="ACHOSC_RS06705"
                     /old_locus_tag="pgaptmp_001341"
     CDS             4244..4423
                     /locus_tag="ACHOSC_RS06705"
                     /old_locus_tag="pgaptmp_001341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000190589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlcI/YnfO family protein"
                     /protein_id="HGU4547317.1"
                     /translation="MATGTTNAKSQALKARVPHEIVNAMESVKESGESTSQFIIASMQ
                     GEIKRRQRRKAKESE"
     gene            complement(4426..4866)
                     /locus_tag="ACHOSC_RS06710"
                     /old_locus_tag="pgaptmp_001342"
                     /pseudo
     CDS             complement(4426..4866)
                     /locus_tag="ACHOSC_RS06710"
                     /old_locus_tag="pgaptmp_001342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309603.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(4924..5112)
                     /locus_tag="ACHOSC_RS06715"
                     /old_locus_tag="pgaptmp_001343"
     CDS             complement(4924..5112)
                     /locus_tag="ACHOSC_RS06715"
                     /old_locus_tag="pgaptmp_001343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001443601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HGU4547318.1"
                     /translation="MLSTDRFIREKECEKLTGLSRSCRYRLEKAGQFPSRRKLGGRSV
                     GWSLSEVLAWKDSCKAVH"
     gene            complement(5487..6716)
                     /locus_tag="ACHOSC_RS06720"
                     /old_locus_tag="pgaptmp_001344"
     CDS             complement(5487..6716)
                     /locus_tag="ACHOSC_RS06720"
                     /old_locus_tag="pgaptmp_001344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HGU4547319.1"
                     /translation="MSRAMNKLSDTLLRKLHGKPAEKNTFYSDGGNLSVKYLTSGKLT
                     WYFTYRAGTGRETRPERIKLGSYPDLSLKAAREKAAQCRTWLAEGKNPRHEMNYTVQE
                     ALKPVTVGDALTYWLEYYVKENRVDYIALKRRLNNHVIQQIGAMPLDKCELRHWLACF
                     DQVAKRTPVTAGFVLQACKQALKFCRRRRYAISNVLDDLNVADVGKKPDISERVLSNK
                     ELGELLQALDKKIFSPYYVALIRLLIVFGARTVELRLSEIGEWDFTEMLWTVPKEHSK
                     TKVAIFRPIPEAILPFVTQLVEQNRHTGLLLGEAKQEASVSQYGRLAHRRLNHPHWSL
                     HDIRRTFTTMLNDLGVDPHVVEQLTGHQMPGMQRVYNHSRYLDAKRNALDMWTERLGI
                     LAGTHENVTTLPVARRK"
     gene            6704..6886
                     /locus_tag="ACHOSC_RS06725"
                     /old_locus_tag="pgaptmp_001345"
     CDS             6704..6886
                     /locus_tag="ACHOSC_RS06725"
                     /old_locus_tag="pgaptmp_001345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001332680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4547320.1"
                     /translation="MLCSRFTPSLRKSDYTSDYTDRCTKGYNVKQAGTRKITNLLIIK
                     NIMHSLKHYETAKNTK"
     gene            6893..7798
                     /gene="tqsA"
                     /locus_tag="ACHOSC_RS06730"
                     /old_locus_tag="pgaptmp_001346"
                     /pseudo
     CDS             6893..7798
                     /gene="tqsA"
                     /locus_tag="ACHOSC_RS06730"
                     /old_locus_tag="pgaptmp_001346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416118.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2 transporter TqsA"
     gene            complement(7823..9211)
                     /gene="pntB"
                     /locus_tag="ACHOSC_RS06735"
                     /old_locus_tag="pgaptmp_001347"
     CDS             complement(7823..9211)
                     /gene="pntB"
                     /locus_tag="ACHOSC_RS06735"
                     /old_locus_tag="pgaptmp_001347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000014033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008750 - proton-translocating NAD(P)+
                     transhydrogenase activity [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Re/Si-specific NAD(P)(+) transhydrogenase
                     subunit beta"
                     /protein_id="HGU4547321.1"
                     /translation="MSGGLVTAAYIVAAILFIFSLAGLSKHETSRQGNNFGIAGMAIA
                     LIATIFGPDTGNVGWILLAMVIGGAIGIRLAKKVEMTEMPELVAILHSFVGLAAVLVG
                     FNSYLHHDAGMAPILVNIHLTEVFLGIFIGAVTFTGSVVAFGKLCGKISSKPLMLPNR
                     HKMNLAALVVSFLLLIVFVRTDSVGLQVLALLIMTAIALVFGWHLVASIGGADMPVVV
                     SMLNSYSGWAAAAAGFMLSNDLLIVTGALVGSSGAILSYIMCKAMNRSFISVIAGGFG
                     TDGSSTGDDQEVGEHREITAEETAELLKNSHSVIITPGYGMAVAQAQYPVAEITEKLR
                     ARGINVRFGIHPVAGRLPGHMNVLLAEAKVPYDIVLEMDEINDDFADTDTVLVIGAND
                     TVNPAAQDDPKSPIAGMPVLEVWKAQNVIVFKRSMNTGYAGVQNPLFFKENTHMLFGD
                     AKASVDAILKAL"
     gene            complement(9222..10754)
                     /gene="pntA"
                     /locus_tag="ACHOSC_RS06740"
                     /old_locus_tag="pgaptmp_001348"
     CDS             complement(9222..10754)
                     /gene="pntA"
                     /locus_tag="ACHOSC_RS06740"
                     /old_locus_tag="pgaptmp_001348"
                     /EC_number="7.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008750 - proton-translocating NAD(P)+
                     transhydrogenase activity [Evidence IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Re/Si-specific NAD(P)(+) transhydrogenase
                     subunit alpha"
                     /protein_id="HGU4547322.1"
                     /translation="MRIGIPRERLTNETRVAATPKTVEQLLKLGFTVAVESGAGQLAS
                     FDDKAFVQAGAEIVEGNSVWQSEIILKVNAPLDDEIALLNPGTTLVSFIWPAQNPELM
                     QKLAERNVTVMAMDSVPRISRAQSLDALSSMANIAGYRAIVEAAHEFGRFFTGQITAA
                     GKVPPAKVMVIGAGVAGLAAIGAANSLGAIVRAFDTRPEVKEQVQSMGAEFLELDFKE
                     EAGSGDGYAKVMSDAFIKAEMELFAAQAKEVDIIVTTALIPGKPAPKLITREMVDSMK
                     AGSVIVDLAAQNGGNCEYTVPGEIFTTENGVKVIGYTDLPGRLPTQSSQLYGTNLVNL
                     LKLLCKEKDGNITVDFDDVVIRGVTVIRAGEITWPAPPIQVSAQPQAAQKAAPEVKTE
                     EKCACSPWRKYALMALAIILFGWLASVAPKEFLGHFTVFALACVVGYYVVWNVSHALH
                     TPLMSVTNAISGIIVVGALLQIGQGGWVSFLSFIAVLIASINIFGGFTVTQRMLKMFR
                     KN"
     gene            11278..12222
                     /gene="ydgH"
                     /locus_tag="ACHOSC_RS06745"
                     /old_locus_tag="pgaptmp_001349"
     CDS             11278..12222
                     /gene="ydgH"
                     /locus_tag="ACHOSC_RS06745"
                     /old_locus_tag="pgaptmp_001349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1471 family protein YdgH"
                     /protein_id="HGU4547323.1"
                     /translation="MKLKNTLLASALLSATAFSVNAATELTPEQAAAVKPFDRVVVTG
                     RFNAIGEAVKAVSRRADKEGAASFYVVDTSDFGNSGNWRVVADLYKADAEKAEETSNR
                     VINGVVELPKDQAVLIEPFDTVTVQGFYRSQPEVNDAITKAAKAKGAYSFYIVRQIDA
                     NQGGNQRITAFIYKKDAKKRIVQSPDVIPADSEAGRAALAAGGEAAKKVEIPGVATTA
                     SPSSEVGRFFETQSSKGGRYTVTLPDGTKVEELNKATAAMMVPFDSIKFSGNYGNMTE
                     VSYQVAKRAAKKGAKYYHITRQWQERGNNLTVSADLYK"
     gene            12408..13790
                     /gene="ydgI"
                     /locus_tag="ACHOSC_RS06750"
                     /old_locus_tag="pgaptmp_001350"
     CDS             12408..13790
                     /gene="ydgI"
                     /locus_tag="ACHOSC_RS06750"
                     /old_locus_tag="pgaptmp_001350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HGU4547324.1"
                     /translation="MEKKLGLSALTALVLSSMLGAGVFSLPQNMAAVASPAALLIGWG
                     ITGAGILLLAFAMLILTRIRPELDGGIFTYAREGFGELIGFCSAWGYWLCAVIANVSY
                     LVIVFSALSFFTDTPELRLFGDGNTWQSIVGASALLWIVHFLILRGVQTAASINLVAT
                     LAKLLPLGLFVVLAMMMFKLDTFKLDFTGLALGVPVWEQVKNTMLITLWVFIGVEGAV
                     VVSARARNKRDVGKATLLAVLSALGVYLLVTLLSLGVVARPELAEIRNPSMAGLMVEM
                     MGPWGEIIIAAGLIVSVCGAYLSWTIMAAEVPFLAATHKAFPRIFARQNAQAAPSASL
                     WLTNICVQICLVLIWLTGSDYNTLLTIASEMILVPYFLVGAFLLKIATRPLHKAVGVG
                     ACIYGLWLLYASGPMHLLLSVVLYAPGLLVFLYARKTHTHDNVLNRQEMVLIGMLLIA
                     SVPATWMLVG"
     gene            13827..14549
                     /gene="folM"
                     /locus_tag="ACHOSC_RS06755"
                     /old_locus_tag="pgaptmp_001351"
     CDS             13827..14549
                     /gene="folM"
                     /locus_tag="ACHOSC_RS06755"
                     /old_locus_tag="pgaptmp_001351"
                     /EC_number="1.5.1.50"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydromonapterin reductase"
                     /protein_id="HGU4547325.1"
                     /translation="MGKAQPLPILITGGGRRIGLALAWHFINQKQPVIVSYRTHYPAI
                     DGLIKAGAQCIQADFSTNDGVMAFADEVLKSTHGLRAILHNASAWMAEKPGTPLADVL
                     ACMMQIHVNTPYLLNHALERLLRGHGHAASDIIHFTDYVVERGSDKHVAYAASKAALD
                     NMTRSFARKLAPEVKVNSIAPSLILFNEHDDAEYRQQALNKSLMKTAPGEKEVIDLVD
                     YLLTSCFVTGRSFPLDGGRHLR"
     gene            complement(14546..14881)
                     /gene="ydgC"
                     /locus_tag="ACHOSC_RS06760"
                     /old_locus_tag="pgaptmp_001352"
     CDS             complement(14546..14881)
                     /gene="ydgC"
                     /locus_tag="ACHOSC_RS06760"
                     /old_locus_tag="pgaptmp_001352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GlpM family protein"
                     /protein_id="HGU4547326.1"
                     /translation="MGLVIKAALGALVVLLIGVLAKTKNYYIAGLIPLFPTFALIAHY
                     IVASERGIEALRATIIFSMWSIIPYFVYLVSLWYFTGMMRLPAAFVGSVACWGISAWV
                     LIICWIKLH"
     gene            15010..15729
                     /gene="rstA"
                     /locus_tag="ACHOSC_RS06765"
                     /old_locus_tag="pgaptmp_001353"
     CDS             15010..15729
                     /gene="rstA"
                     /locus_tag="ACHOSC_RS06765"
                     /old_locus_tag="pgaptmp_001353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416125.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator RstA"
                     /protein_id="HGU4547327.1"
                     /translation="MNTIVFVEDDAEVGSLIAAYLAKHDMQVTVEPRGDQAEETILRE
                     SPDLVLLDIMLPGKDGMTICRDLRAKWSGPIVLLTSLDSDMNHILALEMGACDYILKT
                     TPPAVLLARLRLHLRQNEQATLTKGLQETSLTPYKALHFGTLTIDPINRVVTLANTEI
                     SLSTADFELLWELATHAGQIMDRDALLKNLRGVSYDGLDRSVDVAISRLRKKLLDNAA
                     EPYRIKTVRNKGYLFAPHAWE"
     gene            15733..17034
                     /gene="rstB"
                     /locus_tag="ACHOSC_RS06770"
                     /old_locus_tag="pgaptmp_001354"
     CDS             15733..17034
                     /gene="rstB"
                     /locus_tag="ACHOSC_RS06770"
                     /old_locus_tag="pgaptmp_001354"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     RstB"
                     /protein_id="HGU4547328.1"
                     /translation="MKKLFIQFYLLLFVCFLVMSLLVGLVYKFTAERAGKQSLDDLMN
                     SSLYLMRSELREIPPHDWGKTLKEMDLNLSFDLRVEPLSKYHLDDISMHRLRGGEIVA
                     LDDQYTFLQRIPRSHYVLAVGPVPYLYYLHQMRLLDIALIAFIAISLAFPVFIWMRPH
                     WQDMLKLEAAAQRFGDGHLNERIHFDEGSSFERLGIAFNQMADNINALIASKKQLIDG
                     IAHELRTPLVRLRYRLEMSDNLSAAESQALNRDISQLEALIEELLTYARLDRPQNELH
                     LSEPDLPLWLSTHLADIQAVTPDKTVRIKTLVQGHYAALDMRLMERVLDNLLNNALRY
                     CHSTVETSLLLSGNRATLIVEDDGPGIAPENREHIFEPFVRLDPSRDRSTGGCGLGLA
                     IVHSIALAMGGTVNCDTSELGGARFSFSWPLWHNIPQFTSA"
     gene            17110..18039
                     /gene="tus"
                     /locus_tag="ACHOSC_RS06775"
                     /old_locus_tag="pgaptmp_001355"
     CDS             17110..18039
                     /gene="tus"
                     /locus_tag="ACHOSC_RS06775"
                     /old_locus_tag="pgaptmp_001355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707509.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006274 - DNA replication termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication terminus site-binding protein"
                     /protein_id="HGU4547329.1"
                     /translation="MARYDLVDRLNTTFRQMEQELATFAAHLEQHKLLVARVFSLPEV
                     KKEDEHNPLNRIEVKQHLGNDAQSLALRHFRHLFIQQQSENRSSKAAVRLPGVLCYQV
                     DNLSQAALVSHIQHINKLKTTFEHIVTVESELPTAARFEWVHRHLPGLITLNAYRTLT
                     VLHDPATLRFGWANKHIIKNLHRDEVLAQLEKSLKSPRSVAPWTREEWQRKLEREYQD
                     IAALPQNAKLKIKRPVKVQPIARVWYKGDQKQVQHACPTPLIALINRDNGAGVPDVGE
                     LLNYDADNVQHRYKPQAQPLRLIIPRLHLYVAD"
     gene            complement(18036..19439)
                     /gene="fumC"
                     /locus_tag="ACHOSC_RS06780"
                     /old_locus_tag="pgaptmp_001356"
     CDS             complement(18036..19439)
                     /gene="fumC"
                     /locus_tag="ACHOSC_RS06780"
                     /old_locus_tag="pgaptmp_001356"
                     /EC_number="4.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045239 - tricarboxylic acid cycle
                     heteromeric enzyme complex [Evidence IEA];
                     GO_function: GO:0004333 - fumarate hydratase activity
                     [Evidence IEA];
                     GO_process: GO:0006106 - fumarate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class II fumarate hydratase"
                     /protein_id="HGU4547330.1"
                     /translation="MNTVRSEKDSMGAIDVPADKLWGAQTQRSLEHFRISTEKMPTSL
                     IHALALTKRAAAKVNEDLGLLSEEKASAIRQAADEVLAGQHDDEFPLAIWQTGSGTQS
                     NMNMNEVLANRASELLGGVRGMERKVHPNDDVNKSQSSNDVFPTAMHVAALLALRKQL
                     IPQLKTLTQTLSEKSRAFADIVKIGRTHLQDATPLTLGQEISGWVAMLEHNLKHIEYS
                     LPHVAELALGGTAVGTGLNTHPEYARRVADELAVITCAPFVTAPNKFEALATCDALVQ
                     AHGALKGLAVSLMKIANDVRWLASGPRCGIGEISIPENEPGSSIMPGKVNPTQCEALT
                     MLCCQVMGNDVAINMGGASGNFELNVFRPMVIHNFLQSVRLLADGMESFNKHCAVGIE
                     PNRERINQLLNESLMLVTALNTHIGYDKAAEIAKKAHKEGLTLKAAALALGYLSEAEF
                     DSWVRPEQMVGSMKAGR"
     gene            complement(19582..21228)
                     /gene="fumB"
                     /locus_tag="ACHOSC_RS06785"
                     /old_locus_tag="pgaptmp_001357"
     CDS             complement(19582..21228)
                     /gene="fumB"
                     /locus_tag="ACHOSC_RS06785"
                     /old_locus_tag="pgaptmp_001357"
                     /EC_number="4.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000066594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I fumarate hydratase"
                     /protein_id="HGU4547331.1"
                     /translation="MSNKPFHYQAPFPLKKDDTEYYLLTSEHVSVSEFEGQEILKVAP
                     EALTLLARQAFHDASFMLRPAHQQQVADILRDPEASENDKYVALQFLRNSDIAAKGVL
                     PTCQDTGTAIIVGKKGQRVWTGGCDEAALARGVYNTYIEDNLRYSQNAPLDMYKEVNT
                     GTNLPAQIDLYAVDGDEYKFLCIAKGGGSANKTYLYQETKALLTPGKLKNYLVEKMRT
                     LGTAACPPYHIAFVIGGTSAETNLKTVKLASAKYYDELPTEGNEHGQAFRDVELEKEL
                     LIEAQNLGLGAQFGGKYFAHDIRVIRLPRHGASCPVGMGVSCSADRNIKAKINRQGIW
                     IEKLEHNPGKYIPEELRKAGEGEAVRVDLNRPMKEILAQLSQYPVSTRLSLNGTIIVG
                     RDIAHAKLKERMDNGEGLPQYIKDHPIYYAGPAKTPEGYASGSLGPTTAGRMDSYVDQ
                     LQAQGGSMIMLAKGNRSQQVTDACKKHGGFYLGSIGGPAAVLAQGSIKSLECVEYPEL
                     GMEAIWKIEVEDFPAFILVDDKGNDFFQQIQLTQCTRCVK"
     gene            21427..22602
                     /gene="manA"
                     /locus_tag="ACHOSC_RS06790"
                     /old_locus_tag="pgaptmp_001358"
     CDS             21427..22602
                     /gene="manA"
                     /locus_tag="ACHOSC_RS06790"
                     /old_locus_tag="pgaptmp_001358"
                     /EC_number="5.3.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416130.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004476 - mannose-6-phosphate isomerase
                     activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0009298 - GDP-mannose biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannose-6-phosphate isomerase"
                     /protein_id="HGU4547332.1"
                     /translation="MQKLINSVQNYAWGSKTALTELYGMENPSSQPMAELWMGAHPKS
                     SSRVQNAAGDIVSLRDVIESDKSTLLGEAVAKRFGELPFLFKVLCAAQPLSIQVHPNK
                     RNSEIGFAKENAAGIPMDAAERNYKDPNHKPELVFALTPFLAMNAFREFSEIVSLLQP
                     VAGAHPAIAHFLQQPNAERLSELFASLLNMQGEEKSRALAILKSALDSQQGEPWQTIR
                     LISEFYPEDSGLFSPLLLNVVKLNPGEAMFLFAETPHAYLQGVALEVMANSDNVLRAG
                     LTPKYIDIPELVANVKFEAKPANQLLTQPVKQGAELDFPIPVDDFAFSLHDLSDKETT
                     ISQQSAAILFCVEGDATLWKGSQQLQLKPGESAFIAANESPVTVKGHGRLARVYNKL"
     gene            22703..24211
                     /gene="ydgA"
                     /locus_tag="ACHOSC_RS06795"
                     /old_locus_tag="pgaptmp_001359"
     CDS             22703..24211
                     /gene="ydgA"
                     /locus_tag="ACHOSC_RS06795"
                     /old_locus_tag="pgaptmp_001359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdgA family protein"
                     /protein_id="HGU4547333.1"
                     /translation="MNKSLVAVGVIVALGVVWTGGAWYTGKKIETHLEDMVAQANAQL
                     KLTAPESNLEVSYQNYHRGVFSSQLQLLVKPIAGKENPWIKSGQSVIFNESVDHGPFP
                     LAQLKKLNLIPSMASIQTTLVNNEVSKPLFDMAKGETPFEINSRIGYSGDSSSDISLK
                     PLNYEQKDEKVAFSGGEFQLNADRDGKAISLSGEAQSGRIDAVNEYNQKVQLTFNNLK
                     TDGSSTLASFGERVGNQKLSLEKMTISVEGKELALLEGMEISGKSDLVNDGKTINSQL
                     DYSLNSLKVQNQDLGSGKLTLKVGQIDGEAWHQFSQQYNAQTQALLAQPEIANNPELY
                     QEKVTEAFFSALPLMLKGDPVITIAPLSWKNSQGESALNLSLFLKDPATTKEAPQTLA
                     QEVDRSVKSLDAKLTIPVDMATEFMTQVAKLEGYQEDQAKKLAKQQVEGASAMGQMFR
                     LTTLQDNTITTSLQYTNGQITLNGQKMPLEDFVGMFAMPALNVPVVPAIPQQ"
     gene            complement(24256..>25516)
                     /gene="uidC"
                     /locus_tag="ACHOSC_RS06800"
                     /old_locus_tag="pgaptmp_001360"
     CDS             complement(24256..>25516)
                     /gene="uidC"
                     /locus_tag="ACHOSC_RS06800"
                     /old_locus_tag="pgaptmp_001360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416132.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="glucuronide uptake porin UidC"
                     /protein_id="HGU4547334.1"
                     /translation="KIVAMAVICLTAASGLTSAYAAQLADDEAGLRIRLKNELRRADK
                     PSAGAGRDIYAWVQGGLLDFNSGYYSNIIGVEGGAYYVYKLGARADMSTRWYLDGDKS
                     FGFALGAVKIKPSENSLLKLGRFGTDYSYGSLPYRIPLMAGSSQRTLPTVSEGALGYW
                     ALTPNIDLWGMWRSRVFLWTDSTTGIRDEGVYNSQTGKYDKHRARSFLAASWHDDTSR
                     YSLGASVQKDVSNQIQSILEKSIPLDPNYTLKGELLGFYAQLEGLSRNTSQPNETALV
                     SGQLTWNAPWGSVFGSGGYLRHAMNGAVVDTDIGYPFSLSLDRNREGMQSWQLGVNYR
                     LTPQFTLTFAPIVTRGYESSKRDVRIEGTGILGGMNYRVSEGPLQGMNFFLAADKGRE
                     KRDGSTLGDRLNYWDVKMSIQYDFMLK"
BASE COUNT         6147 a         6687 c         6078 g         6604 t
ORIGIN      
        1 acctgatgcc gttccttgtc tgtctccacc agcagcatga cttcacccca tcgcgccgcc
       61 gccctccggt aaaagccttt cgcctcgagt tcttccgcta tgcggtcatg taccatcgcc
      121 accccctcag aacggaatac cgtcaccgta agggtcatcg cctcccgctg gtggctgatt
      181 accctgtgtg cctgtggttt tgcgtctgtt cccgccagga agtgccgcac gggcactgat
      241 tacgctgtct gcaataacct gataaccctg ccgcgtttcc ccattctgtc cggtccactg
      301 gctgacctgc atcgtgccgg atacgctggc aacgtcgcct ttttgatgtt tagccaggaa
      361 gtcggcctgc ttaccaaatg cgatgaccga tagccataac gtcgcctgcc catcctgcgc
      421 ctgactacat ggcagcgata ccgccatacg cgccagcgtc atcggtgtac ccttgctggt
      481 ctgttttacc tgcgggtcgt ccaccagccg cccgtaagct gctatctgtg ctgtcatgat
      541 tccacctctc cggttttaac gttgatggtt gttacctgtt ccgcttcggc aatctcccgc
      601 tctgtcagcg tggcaaaatt tgccgccgtc gtggtcatga atgcgcttat cagttcggga
      661 tgtgctttcg cgtatccttc ccccgcgtgg cggtctatcg ttctgattgc cacctttaaa
      721 gcgtgctctg tcatgtctaa cgcgcgatat ttcggttctg ttctgtctct gcgttttttg
      781 agtgatttat taaatttccc tgaagtgtgc atatttattt ttaccccctc gtttaaaaag
      841 ttttgagttg tgcctcccct tgtctacctt atctacctta gtggccctca taccagtaat
      901 ggcgcggctt tcagctgggt agagtgcttt tatccactat ctaccccgtg tctacctccc
      961 tgtctgattc aggtaaaatc aggtagagag ggtagatagt gggtagacag taaaaaaagg
     1021 ctatctacct aacttaatgc actgaattaa atgtattttt ctttactcag gtagacaggg
     1081 tagacagcaa ttacaaaaaa ttataaaaac gcgtcgcact cgtctgttgt tattgcgtta
     1141 gtctgcgtta ctcccttaac tttccgcgta atatattcat gtccgtaaac tttcgcggct
     1201 ggcttcatag ccttgccaaa gtcatttacg tttagcggtt tgctcctgcc tgcgtacgcc
     1261 ataaacgcca gatagacgcg gtaaaggctg tttctggtcg tgtacttcac tgaatcgcca
     1321 ccgccgccca tcatcaggcc gcgtgcttcc tccagaaaat tcaggaactg gcaaaactca
     1381 ataaccggat ccgtctgttg ctttattgcc agagcttcat caccgtcacg ctgttccagt
     1441 agtaaagccc gtgccttctc agggtcggta aagttcgcca gcaatcggcg gataatgaca
     1501 gggatttcag ccgcgatctt ttccggtagc tccctgtctt tttcggcctc actgacgata
     1561 ttgtcgaaac ggaaaatcac gcgacgacgt gccacacctc cggcccgttc ggtgaatatc
     1621 atcgggttat tgttggtcgc cagcaccacc gccctgatta cagccgtgaa acgcttttcg
     1681 tatttcgggt taatttccac ggggtcaccg cccgtgattt tcttgatgcc cgttccttcg
     1741 cctgtatatt tcggctggtc agccaggacg ataagacgac tcccgacaac ctgcgcacgt
     1801 ccaccagcat catcaagcga tgtcatttca gcgcttaccg tgttctgttt ccctgccaga
     1861 aggctggcta tgtgcgtgaa tgtactttta ccgctcccgc cgtctccggt ggcctcaata
     1921 aacatctgcc agtcgtaccg gttcgccata atcatgtaca gcgcggcaca tatacgcatc
     1981 atcttgcgcg ggtcttttcc ggctgcgtgc tcaagccatt tatgaaagtt tggcgcgtta
     2041 tcgcggatgt tctcccctgg tgctggtggc gtgtactcaa tgccgttgtg cgtggtgatc
     2101 cagttctccg gcgtgtgcgg ggaaaattcc cccgttttca ggtcaagcgc accattggcg
     2161 aacggcagca aatcgccgga cggctcgccc attggttcgg caataacttt taacgcttcc
     2221 acggcgttat tgattacgcg cttgctgaaa gtggccctgt gctctgaata gatcgccacc
     2281 atttcgcggc taagttccat tgtgctgacc ggacaccata ccccgccgcg ccatacgtga
     2341 acgatttcac tttcaggatg tacgcaaacg ccatcaaagc gatcggcaag cagctgcgcg
     2401 cgctcactgt ccgccatctg cgaaagttgc gccttttgct ttaccggaag ctcaatgacc
     2461 agaccatcag aaagattctg gcgctcacga gccagatatt cgcgccagtt ctccaccttc
     2521 tgaccgtgca taccatcagg gtaaaaattt gcatcctgta cgcctgccgc cgccagcttc
     2581 tgaccaatcg cctttatcat tactggcgca agatatccgg ccctgaatat gcgcacggat
     2641 tttctgcctt ccggcacaat ttgcagctta tccagttcgg ataactgctg ctccccaagc
     2701 cacacaggag gctcattatc tccggccata cgcgcgtcat gttcctgcca ttgttttgcg
     2761 tgtgaccagg catcactacc cgcaaaaata atgacttctg tttctttgtg ttttatgccg
     2821 cgtgactgct gttttacgtt cggtgccagt ttcatttttt acccctgaat acgttaagca
     2881 tcttttttat ttcctgaata ttggcgcgtg ctttcttcct gctggttggt ttactgcggg
     2941 gcgctgcctg taccagagaa aaatcacgcc gaaactgata aacaggcatc acgcaatcat
     3001 attcgtagcc ttcacggcgg taggttacgc gccgttcttc cacgccctta atcattaccg
     3061 tgccgccgta ctggtcgcgg taaatatcac cgcgcgtaaa tttagggtga gtgttgccac
     3121 tggcagttaa gccagaatat ttaagtttca ttatttttat tctccggtgt gctgttcttt
     3181 atatctgtcg tgcaatagat ctatttcttg cagttccatt attacaggct caagaagcgt
     3241 tattaatgcc gtggcaattc ttgatttttg tttgtcgcgt tcattgtcgc caagtgtttc
     3301 aagccatatg cgcaatattt ccagcatgtt ttcactgtga gaaagtgcaa gaaatgcgcg
     3361 gtatattgtt tcgtggtaaa tatcacgcat ggcttacatc ctcaggaaat tttcttctgt
     3421 aatgcgcctc tgccacatat tccgcataat cggcggcgat attcagtaca tcaagccccg
     3481 ttgatttata ttctctcgtg gaaagtaaga aaaaagccgc tctaataagc tctggcattg
     3541 acgaaagcgc atcagccgca tcatcaggaa cgccggaaaa ttcctgtttc agggaattaa
     3601 aacgatcatc acgcataacc acccccattt tcacaatcag caatcaggat ggctttagcc
     3661 tcattcagcg ccatatcagc actaagttgc ataaccgcca gcgaatgagg aacgaaagcc
     3721 ccggcatatt ctgtttcact ggtgtcgtgc ttatgcgccc tgtcagcaat aacagaaata
     3781 tcaatcagcg catgcattag cgttatgatg gcttcggcgg ctgcgtccgg acgggtgtta
     3841 ttgcacatgg cacacctcct gacgaatacg ggcagcgaat accatcacgc agccagccgg
     3901 agattgctgg cgtgcttcct gttcgctggt ggcctcaatg gtaatcacgc gcggttgtgc
     3961 cgtgctcagg gcgataaaac gccagatgta tttattcagg tcgtgcgagt cccgcccttg
     4021 cgggtgtgtg gtatgatttc tcatagctac ctcgatactt tcgctatcgt tggtggttag
     4081 aagccctgcg agtggtaacg acacttgcgg ggctttgcat ttatgcacct tgataatctc
     4141 aaggtgtggc ccactatatg ctttaggtgt ggcccacgtc aagggtttta tttgtgcttt
     4201 ttctgtgtat actgtccccc accaatccaa cagaggaata gaaatggcaa cgggtacaac
     4261 aaacgcaaaa tcacaagctc taaaggctcg tgtaccacac gaaatagtaa acgccatgga
     4321 atcagtgaaa gaatcaggcg aaagcacatc acaattcatc attgcgtcaa tgcaaggcga
     4381 gatcaaacgc cgccagcgcc gcaaggccaa agaatcagaa taatcactat cagcgccgtg
     4441 gtgtgaggta ttacggcgca ttgctatgca ggacaacaca atgaccgata aagaattgac
     4501 caaaacatta tcaccggcac ggaaaagacg gcgcagaaag atagaacatg aatcagaaag
     4561 attcgcgcca tgtgcttttg cccttgagca attccttaaa gagtacaggg aaaagcgctc
     4621 attgcaggta tggcaacgaa ctgaaccaga ctgattgcat tgcccaccag cctgatagcg
     4681 gctatcatcc ccgtgcttat gtttgggatc acacacacaa acggcgcagc gggttatctg
     4741 ttcagaaagg cggctccatt tcggggccgc tttttttatg cctgaaaaac cccaattttg
     4801 tggttttcca gtctcaccag ggcgaacgaa tccccgccca cgttctggcg tatattcaat
     4861 cttcatggtt atagctctgt gttcagatga ttgatgtgtg gcggctgtgt gccgccagcg
     4921 tgattaatga actgccttgc agctatcctt ccaggccaga acctcggata aagaccagcc
     4981 aacggaacga ccgccaagtt tacgacgtga tgggaattgt ccggcctttt ccaggcggta
     5041 gcggcatgag cggctaaggc ctgttagctt ttcgcattct ttttcacgta taaaccgatc
     5101 agtgcttaac actattgccc cctttcgttt cttaaagagt tatttcgtgt tctattgcgt
     5161 tgggatgtgt ctgattgtgt caggatgatt cagagttggc aaatgttgag gtcgtatggt
     5221 ttacagaaac aggaataatc aggataaaat catttaaatt catgttaata caaaggcata
     5281 aaatattgtt tcatgccttt tttctcgcca tttaaagagt gattcgctag tgtataaaaa
     5341 accagtaaca cattaaaaat cagccactta taaatccgta cactttttcg cctcttgttc
     5401 gtagttgttc cgcattgttg ctcattgttg catgttgtat ctgttcgcat atccagtatg
     5461 cgcatactga aaaaacacga aaaaaattat tttcttctgg ctactggtag cgtggttacg
     5521 ttttcatgtg ttcccgccag tatccctaac cgctccgtcc acatatccag cgcattgcgt
     5581 ttagcatcca gataacggga atgattataa actcgctgca ttcctggcat ctgatgacct
     5641 gtaagctgct ccacgacatg cggatcaacg cctaaatcgt tcagcatggt tgtaaaggtg
     5701 cgccggatgt catgcagtga ccagtgagga tgattaagcc tcctgtgcgc taatcttcca
     5761 tactgcgata cgctggcctc ctgtttcgct tcccccagca ataagcccgt gtgcctgttc
     5821 tgctccacca gctgcgtgac gaacggcagg atcgcttccg gtatgggccg gaatattgcg
     5881 accttcgttt tgctgtgctc cttcggaacg gtccatagca tttccgtaaa atcccactcc
     5941 ccgatctccg atagcctcag ttctaccgtc ctggctccga agacaatcag gaggcggatt
     6001 aacgcgacgt agtaagggga aaatattttt ttgtccagtg cctgcaataa ttcgccaagt
     6061 tctttgttac ttaagacacg ttcgcttata tccggttttt tcccaacgtc cgccacgttc
     6121 agatcgtcca gaacgttgct gattgcatag cgccgcctac ggcagaactt aagcgcctgt
     6181 ttgcacgcct gtagcacgaa tccggcagta acaggcgttc gctttgccac ctggtcaaaa
     6241 caggccagcc agtgccgtag ctcgcattta tccagcggca tagcaccaat ctgctgtatt
     6301 acgtgattat taagtcgcct tttcagggcg atataatcca cgcggttttc ctttacgtaa
     6361 tactcaagcc agtaggtgag cgcatcgcca accgttacgg gctttaacgc ttcctgtacg
     6421 gtgtaattca tctcatgacg tggatttttc ccctcagcca gccatgtgcg acactgtgcg
     6481 gctttttccc tggctgcttt caggctcaga tcaggataac ttcccagctt aatgcgttcg
     6541 ggtcgtgtct cccttcccgt tccggccctg tatgtgaaat accaggtcaa tttccctgat
     6601 gttaaatatt tcacgctcag gtttccgcca tcactataaa acgtgttttt ctccgctggc
     6661 ttaccatgaa gtttccttag caaggtatcg ctcagtttgt tcattgctct gctcacgttt
     6721 taccccctct ttacgaaaaa gtgactacac cagtgactac accgatcgtt gcacaaaggg
     6781 ctacaacgtg aaacaagctg gaacaaggaa aatcacaaat ctgttgataa tcaaaaacat
     6841 aatgcacagc ctgaaacatt atgaaacagc aaaaaacact aaatgataaa atgctgttat
     6901 tcttaacccg ctggtgcaac acatggtccg ctggcgagtg ccgcgtgtac tggcggtgtc
     6961 gattttgatg accatcatcg tgatggcgat ggtgttgcta ttagcttatc tgggttccgc
     7021 gctcaacgag ttgacgcgga cgttaccgca atatcgcaac tctattatga cgccgctgca
     7081 agctcttgag ccgttgttgc aacgcgtagg gattgacgtc tcagttgacc agctggcgca
     7141 ttacattgat ccgaacgcgg cgatgacgtt gctcaccaac ttattgacgc agttatctaa
     7201 tgccatgtca tcaatatttt tattgctgct gacggtgctg tttatgctgc tcgaagtgcc
     7261 acaattgccc ggaaaatttc agcaaatgat ggcgcgtccg gttgaaggga tggcggcgat
     7321 tcaacgtgca attgacagtg tttctcatta tctggtgctg aaaacagcca tcagcatcat
     7381 caccggcctg gtcgcctggg cgatgctcgc cgcactcgat gttcgcttcg cttttgtctg
     7441 gggattgctg gcctttgcgc ttaattacat ccctaatatt ggttcagttc tcgcggcaat
     7501 cccccctatc gctcaggtac tggtgtttaa tggcttctac gaagcgttgc tggtgctggc
     7561 gggatatctg ctgattaatc tggtcttcgg caatattctg gagccgcgca tcatgggacg
     7621 tgggctgggg ctttccacat tggtggtatt tttgtcgttg attttttggg gatggttgtt
     7681 aggaccggtg ggtatgctgc tttccgtgcc gttaacaatt attgtcaaaa ttgcgcttga
     7741 acaaacagcg ggaggtcaaa gcatcgccgt tctgttaagc gatctcaata aagagtgacg
     7801 gcctccgcag aggccgtcag ggttacagag ctttcaggat tgcatccacg ctggctttgg
     7861 cgtcaccaaa cagcatgtgg gtgttttcct tgaagaacag cgggttttgc acaccagcat
     7921 agccagtgtt catcgaacgt ttaaagacaa tcacgttctg cgctttccac acttccagca
     7981 caggcatacc agcaatagga ctcttcggat catcctgcgc cgccgggtta accgtatcgt
     8041 tagcaccaat caccagtacg gtatcggtat cagcaaagtc atcgttgatc tcgtccattt
     8101 ccagcacgat gtcatacggt acttttgctt cagccagcaa tacgttcata tgtccaggca
     8161 aacgccccgc aaccgggtgg ataccgaaac gcacgttgat accacgagcg cgcagtttct
     8221 cggtaatttc agcgacagga tattgcgcct gcgcgactgc catgccgtac cccggagtaa
     8281 tgatcactga atgggagttt ttcagcagtt ccgctgtctc ttctgcggtg atttcgcggt
     8341 gctcgcccac ttcctgatca tcgccagtag aagagccgtc ggtgccgaaa ccacccgcaa
     8401 taacgctgat aaaggaacgg ttcatcgcct tacacataat gtaagaaagg atagcgcccg
     8461 aagaaccgac cagcgcaccg gtcacaatca gcaggtcgtt gctgagcata aagcccgcag
     8521 ccgcagccgc ccagccggag tacgagttca gcatcgacac caccactggc atatctgcac
     8581 caccgatgga ggcgactaaa tgccagccga ataccagcgc aatggcggtc attatcagca
     8641 atgccagcac ttgcaggccg acgctatccg tgcgaacaaa tacaatcagc agcaggaagg
     8701 aaacgaccag agccgccagg ttcattttgt gacggtttgg cagcatcaac ggtttagacg
     8761 aaatcttgcc acacagtttg ccgaacgcca ccaccgaacc cgtgaacgtt accgccccga
     8821 tgaagatacc gaggaacact tccgtcaggt gaatattgac cagaatcggt gccattcccg
     8881 cgtcatgatg cagataactg ttaaagccaa ccagcactgc cgccagaccc acgaagctat
     8941 gcaggatcgc caccagttct ggcatttcgg tcatttcaac tttcttcgcc agacggatac
     9001 caattgcccc accaatgacc atcgccagca agatccagcc aacattaccc gtatccggtc
     9061 caaaaatggt tgcgattaac gcaatcgcca tcccggcgat accgaagttg ttaccctggc
     9121 gagacgtttc atgtttcgaa agaccggcca gactgaagat aaacaggatc gcggcaacaa
     9181 tgtatgcagc tgtaactaat cctccagaca tatgttaccc cttaattttt gcggaacatt
     9241 ttcagcatgc gctgagtcac ggtgaagcca ccgaaaatat taatgctggc tataagcacc
     9301 gcgataaaac taaggaagct aacccagccg ccctggccaa tctgcaacag tgctccgaca
     9361 acaataatcc ctgaaatcgc gttggtgacc gacatcaacg gtgtatgcag cgcatgcgat
     9421 acattccaca ccacgtaata accgacaacg caggccagcg cgaaaacggt gaagtgccca
     9481 agaaactctt tcggcgcaac gcttgccagc caaccgaaaa ggatgattgc cagcgccatc
     9541 aatgcgtatt tacgccacgg tgagcaggca catttttcct cagttttcac ttccggtgcc
     9601 gctttttgtg ccgcctgcgg ctgagctgat acctgaatcg gcggtgccgg ccaggtaatt
     9661 tcgcccgcac ggatcacggt cacgccgcga atcaccacat catcaaaatc aacagtgata
     9721 ttgccgtctt tctctttgca caacagtttc agcagattaa cgaggttagt accgtaaagc
     9781 tgtgaggatt gcgtcggcag acggcccgga agatcggtat aaccaatcac tttgacacca
     9841 ttttccgtag tgaagatttc acccggcacg gtgtattcac agttgccacc gttttgggct
     9901 gccaggtcga caatcacact gcccgccttc atggagtcaa ccatttcacg ggtaattagc
     9961 ttcggcgctg gtttgcctgg aataagcgcg gtggtgacaa tgatatcgac ctcttttgcc
    10021 tgggcggcaa agagttccat ttccgctttg atgaacgcgt ccgacatcac tttggcatag
    10081 ccatcgccgc tgcccgcttc ctctttaaaa tccagctcga ggaattccgc gcccatactt
    10141 tgaacttgtt ctttcacttc cgggcgggtg tcgaatgcac gcacaatcgc gccgagactg
    10201 tttgctgcgc caatggcggc cagacctgca acacccgcac caatcaccat cacttttgcc
    10261 ggtggcactt tcccggccgc agtaatttgc ccggtaaaga agcgcccaaa ttcatgtgcc
    10321 gcttcaacaa tggcgcgata accggcgatg ttcgccatcg agcttagtgc gtccagcgat
    10381 tgtgcgcgtg agatacgcgg cacagaatcc atcgccatca cggtcacgtt acgttccgca
    10441 agtttttgca ttaattccgg attctgcgca ggccagataa aactcaccag cgttgtccca
    10501 ggattcagta acgcaatttc atcatctaac ggcgcattga ccttcagaat gatctctgac
    10561 tgccagacgc tattcccttc tacaatttca gcgcccgctt gcacaaacgc tttatcgtca
    10621 aaacttgcca gttgacccgc gccgctctct accgcgacgg taaaacccag tttcagcagc
    10681 tgttccactg tttttggcgt tgctgcaaca cgggtttcat tggttaaccg ttctcttggt
    10741 atgccaattc gcatgatatt cccttccatc ggttttattg atgatggttt gcctgtgtca
    10801 ggagccacac aagccgctca tgtacgagct aaatgttact ccgttaaaat aaattagtaa
    10861 caaacgccct ataacgtact gaaaattatg cctgtgatct agcgccaaaa atcagtattt
    10921 cggcgtgaac tcgcaaaata ttaacgattc agccgtgata gtgggataaa caccttagaa
    10981 cgccggataa agactgataa ttgtcttcga cggtcgggta aaacgagaca tcgccccggc
    11041 acgaatcact acttaacatt aaattaactt atacaattca gttgcttcag tagtaatgat
    11101 gctgatacgg ctgtttttta agcatagacg gtcatttgag caggattaaa attggcttaa
    11161 ggaatgtgat atgaaaaatg acgcagacag ttacaccgtt taaatgcaat aatcagccac
    11221 gtttctcgtt aataacaata ccagtacctg gtttgcgcaa ggcgaaggat tatttttatg
    11281 aagcttaaga acaccctcct ggcgtcggca ctgctttctg ctacggcatt ctccgttaac
    11341 gcagcaacag aactgacacc ggagcaagcg gcagcggtta aaccttttga ccgtgtagtg
    11401 gttaccggtc gttttaatgc tattggcgaa gcggtgaaag ccgtttctcg tcgcgcagat
    11461 aaagaaggtg ccgcctcttt ttatgttgtc gacacttctg attttggtaa cagcggtaac
    11521 tggcgtgtgg tcgctgacct ctataaagcc gatgctgaaa aagcagaaga aacaagtaat
    11581 cgcgtaatta acggtgttgt cgaactgccg aaagatcagg ctgttctgat tgaaccgttt
    11641 gacacggtca ccgtccaggg cttctatcgt agccagccag aagtcaatga tgccatcacc
    11701 aaagcagcaa aagcgaaagg tgcctactct ttctacattg ttcgtcaaat cgatgccaac
    11761 cagggcggca accagcgtat tactgcattc atctataaaa aagatgctaa gaaacgtatc
    11821 gtccagagcc cggatgtgat cccggcagat tccgaagcag gacgtgcagc tctggctgcc
    11881 ggtggcgaag ccgcgaagaa agttgagatc ccaggtgttg cgactaccgc atcaccaagt
    11941 tctgaagtcg gtcgtttctt tgaaacccag tcatcaaaag gcggacgtta cactgtcacg
    12001 cttcctgacg gcactaaagt cgaagaactg aacaaagcga cagcagcgat gatggtcccg
    12061 ttcgacagca tcaaattctc tggcaactac ggcaacatga ccgaagtctc ctatcaggtt
    12121 gcgaaacgtg ccgcgaagaa aggtgctaag tattaccaca tcacccgcca gtggcaggaa
    12181 cgtggtaata acctgaccgt cagcgcagat ctgtataaat aactattcag ttactcctat
    12241 aaggcggctt gatgccgcct ttcgcatttt tagtcacaaa tttccccgcg accattgcat
    12301 gcagacacat ctcttcgtaa aatcccgcgc ttcagtggca aactccattt ttatgcgttt
    12361 tgccctaata tttattcttc ttatcacgtt ttaatcactg gatatcgatg gaaaagaaac
    12421 tgggactgag cgcactcacc gcgctggtat taagctcaat gctgggcgcg ggtgttttca
    12481 gtctgccgca aaatatggcg gcagttgcca gcccggcagc actgctcatc ggctggggta
    12541 ttactggcgc tggcatttta ttgctggcct ttgccatgct gatcctcacg cgcattcgtc
    12601 ccgaacttga cggcggtatc tttacctatg cccgcgaagg gtttggcgag ctaatcggct
    12661 tttgttccgc atggggatac tggctgtgcg cagtcatcgc caacgtctcc tatctggtga
    12721 tcgttttttc cgcgttaagc ttttttacgg acacgccgga attgcgcctg ttcggcgatg
    12781 gtaatacctg gcaatcgata gtcggtgcat cggctttatt gtggattgtt cactttttga
    12841 tcctgcgcgg ggtgcaaacc gctgccagca ttaacctggt ggcgacactg gcaaaattgt
    12901 tgccgctggg tctgtttgtt gtgctggcga tgatgatgtt caaactggat accttcaagc
    12961 tcgacttcac cggacttgca cttggcgtac ccgtgtggga acaggtgaaa aacaccatgc
    13021 tgatcaccct gtgggttttc attggtgtgg aaggtgccgt ggttgtttct gcgcgtgcgc
    13081 gtaataaacg tgatgttggc aaagcgacac tgctggcggt tctctccgct ctgggcgttt
    13141 acttgctggt aacgctgctt tcgctgggcg tggtggcacg tcctgaactg gctgaaattc
    13201 gtaacccgtc aatggccggt ctgatggtgg aaatgatggg accatggggc gaaatcatca
    13261 tcgctgccgg tttgatcgtt tccgtttgcg gcgcgtactt aagctggacc atcatggcgg
    13321 cagaagttcc gttcctggct gccactcata aagcattccc acgcattttt gcgcgccaga
    13381 acgcacaagc tgcgccatcc gcctcgctgt ggttgaccaa catctgcgtg caaatttgtc
    13441 tggtactcat ctggctgacc ggttccgatt acaacacgtt gctgaccatc gcctcagaaa
    13501 tgattctggt gccctatttc ctggtcggcg cgttcttgct gaaaatcgca acccgtccac
    13561 tgcataaagc ggtaggtgtc ggtgcctgca tttatggctt atggttattg tatgcctctg
    13621 gtccaatgca cttgctgtta tccgttgttc tgtatgcgcc ggggctgctg gttttcctgt
    13681 atgcgcgcaa aacgcataca catgataatg tactgaaccg tcaggaaatg gtgctgattg
    13741 gcatgctgtt aattgcctca gttccggcga cctggatgct ggtgggataa cgttcatacc
    13801 atcgttggat agcaaaggag ataacgatgg gtaaagccca gcccttgcca atattaatta
    13861 ctggcggagg tcgtcgcatc ggcctcgccc tcgcctggca tttcattaat caaaagcaac
    13921 cggtgattgt cagctatcgg acgcactatc cagccattga tggactgatt aaggcaggtg
    13981 cgcagtgtat tcaggctgat ttttcgacca acgacggtgt gatggcgttt gccgatgaag
    14041 tactaaaaag cacccatggt ctgcgtgcta ttttgcataa cgccagtgcg tggatggcgg
    14101 aaaaaccagg tacaccactg gccgacgtac tggcttgcat gatgcagatc cacgttaata
    14161 ccccatacct gctcaaccat gcgctggaaa gattactgcg tgggcacgga cacgccgcca
    14221 gcgatatcat tcactttacc gattatgtgg tggagcgcgg tagcgacaaa catgttgcgt
    14281 atgctgcaag caaagcggca ctggataata tgacccgctc gtttgcccgc aagctggcac
    14341 cggaagtgaa agtgaattct attgcgccat cgctgatcct gtttaatgaa catgatgatg
    14401 ccgaatatcg acaacaggcg ctgaataaat cactgatgaa aaccgcgcct ggcgagaaag
    14461 aagtgatcga cctggtcgat tacttactta ccagctgctt tgtcaccgga cgcagtttcc
    14521 cacttgatgg cggtcgtcat ctgcgttaat gcagctttat ccagcatata atcaacaccc
    14581 atgcgcttat cccccaacac gcaacagaac caacaaaagc ggcgggcaag cgcatcattc
    14641 cggtgaaata ccacagcgac accaggtaaa caaaataggg aataatcgac cacatactaa
    14701 aaatgatggt tgcgcgtaag gcttcaatgc cgcgttcgct ggcaacaata taatgcgcga
    14761 taagcgcaaa ggtcgggaaa agtggaatca gcccggcgat ataataattt ttcgtttttg
    14821 ctaaaacacc aatcaacagc actaccagcg caccgagcgc ggctttgatt accagcccca
    14881 tctttttacc ttaacacttc cataacaagt catcagtaga atacctgatg aaaacttgtt
    14941 tagaaacgat tgatagtaag taaaaacagc gcggtgtatt gtgacgtttt tatatctacc
    15001 gtgaatgtta tgaacactat cgtatttgtg gaagatgatg cggaagtcgg ttcactgatt
    15061 gccgcgtacc tggcaaaaca tgatatgcag gttaccgtag aaccgcgcgg cgaccaggcc
    15121 gaagaaacca ttttgcgaga aagtccggat ttggtgttac tcgacatcat gctaccaggc
    15181 aaggacggca tgaccatttg tcgtgattta cgcgcaaagt ggtctggacc gattgttctt
    15241 ctaacctctc tcgatagcga tatgaaccac atcctggcac tggaaatggg tgcctgcgac
    15301 tatattctca aaacgacgcc ccctgctgtt ttgctagcgc gtttacgttt gcatttgcgt
    15361 cagaatgagc aagccacact gaccaaaggt cttcaggaaa cgtctctgac tccctacaaa
    15421 gccctgcatt tcggcacgtt gaccatcgat cccatcaacc gcgtagtcac cctggctaac
    15481 actgaaatct cgctctcgac agctgatttc gaattattgt gggaattagc tacccatgcc
    15541 gggcaaatca tggaccgcga tgcattgctg aaaaatttac gcggcgtcag ttatgacgga
    15601 ctggatcgta gcgtggacgt ggctatttcg cggttaagaa aaaaactgct cgataacgcc
    15661 gcagaacctt atcgcattaa aactgtgcgt aacaaaggct atctttttgc gcctcatgca
    15721 tgggaataag cgatgaaaaa actgtttatc cagttttacc tgttattgtt tgtctgcttc
    15781 cttgtgatgt ctctgctggt tgggttggtg tacaaattta ccgccgaacg cgcgggcaaa
    15841 cagtcgctgg atgatttgat gaacagttcg ctgtatctga tgcgcagcga attgcgtgag
    15901 atccccccac acgactgggg taagacgctg aaagagatgg atttaaatct ctctttcgat
    15961 ctgcgtgtcg agccactgag taaataccat cttgatgata tttccatgca ccgactgcgt
    16021 ggcggcgaaa ttgtcgccct ggacgatcag tacacgtttt tgcagcgtat cccgcgcagc
    16081 cactacgtgc tggcagttgg tcctgttcct tatctttatt atctccatca gatgcgattg
    16141 ctggatatcg ccctgatcgc ttttattgct atttccctcg cctttccggt gtttatctgg
    16201 atgcgtccgc actggcagga tatgttaaaa ctggaagcag cggcgcaacg atttggcgat
    16261 gggcatctca atgagcgtat ccactttgat gagggttcga gctttgaacg acttggtatc
    16321 gcatttaacc agatggcgga caatatcaac gccttgattg ccagcaaaaa acagcttatt
    16381 gacggtatcg ctcacgaact gcgaacaccg ttagtgcgcc tgcgttatcg actggagatg
    16441 agcgataacc tgagcgccgc cgaatcccag gcgttgaatc gtgatatcag tcaacttgaa
    16501 gctttaattg aagagctgct gacttatgcc cgactcgatc gcccacaaaa cgagcttcat
    16561 cttagcgaac cagacctgcc gttgtggctg tcaacgcatc tggcagatat tcaggcagta
    16621 acgcccgata aaacggtacg gataaaaacg ctcgtgcaag gccattatgc ggcgttggat
    16681 atgcgcttaa tggagcgcgt gctggataat ttgctcaata acgccctgcg gtactgccat
    16741 tcaacggttg aaaccagcct gctactgtcg gggaatagag cgacattaat tgttgaggat
    16801 gatggcccag ggattgcccc agaaaaccgc gaacatatct ttgaaccttt tgttcgcctc
    16861 gatcccagcc gggatcgctc aaccggcggc tgcgggctgg ggctggcaat tgtccactct
    16921 atagcactgg caatgggcgg tacggttaat tgtgacacca gcgaactggg tggtgctcgc
    16981 ttctcgttta gctggccgtt atggcataac atcccgcaat ttacctctgc ctgacactac
    17041 gcgcacgatg gtcaagtcac cacgactgtg ctataaaata agtatgttgt aactaaagtg
    17101 gttaatatta tggcgcgtta cgatctcgta gaccgactca acactacctt tcgccagatg
    17161 gaacaagagc tggctacatt tgccgctcat cttgagcaac acaagctatt ggttgcccgc
    17221 gtgttctctt tgccggaggt aaaaaaagag gatgagcata atccgcttaa tcgtattgag
    17281 gtaaaacaac atctcggcaa cgacgcgcag tcgctggcgt tgcgtcattt ccgccattta
    17341 tttattcaac aacagtccga aaatcgcagc agcaaggccg ctgtccgtct gcctggcgtg
    17401 ttgtgttacc aggtcgataa cctttcgcaa gcagcgttgg tcagtcatat tcagcacatc
    17461 aataaactca agaccacgtt cgagcatatc gtcacggttg aatcagaact ccccaccgcg
    17521 gcacgttttg aatgggtgca tcgtcatttg ccggggctga tcacccttaa tgcttaccgc
    17581 acgctcaccg ttctgcacga ccccgccact ttacgctttg gttgggctaa taaacatatc
    17641 attaagaatt tgcatcgtga tgaagtcctg gcacagctgg aaaaaagcct gaaatcacca
    17701 cgcagtgtcg caccgtggac gcgcgaggag tggcaaagaa aactggagcg agagtatcag
    17761 gatatcgctg ccctgccaca gaacgcgaag ttaaaaatca aacgtccggt gaaggtgcag
    17821 ccgattgccc gcgtctggta caaaggagat caaaaacaag tccaacacgc ctgccctaca
    17881 ccactgattg cactgattaa tcgggataat ggcgcgggcg tgccggacgt tggtgagttg
    17941 ttaaattacg atgccgacaa tgtgcagcac cgttataaac cacaggcgca gccgcttcgt
    18001 ttgatcattc cacggctgca cctgtatgtt gcagattaac gcccggcttt catactgccg
    18061 accatctgtt ctggccgtac ccagctgtca aactcggctt cgctaagata ccccagcgca
    18121 agggccgcag cttttaaggt cagcccttct ttatgcgctt ttttggcgat ctccgcggct
    18181 ttgtcataac caatgtgggt gttaagcgca gtcaccagca tcagcgattc attgagtaat
    18241 tgattgattc gctcacgatt cggttcaata cccactgcgc agtgtttgtt aaaactttcc
    18301 atgccatctg ccagcaagcg caccgattgc aggaaattgt ggatcaccat tggacggaag
    18361 acgttcagtt caaagttacc ggaagcgccc cccatgttga tcgccacgtc gttccccatc
    18421 acctgacagc agagcatggt taatgcctca cactgtgttg gattcacttt ccccggcatg
    18481 attgagctgc ccggctcatt ttccgggatt gagatttcac caattccgca gcgcgggcca
    18541 gaggccagcc agcggacatc attggcgatt ttcatcagtg acacagccaa ccctttcaac
    18601 gcgccgtgcg cctgaaccag ggcatcacag gtcgccagcg cttcaaattt gttcggcgcg
    18661 gtaacaaacg gtgcacaggt aatgactgcc agttcatctg ctacgcgacg cgcatactcc
    18721 ggatgggtat ttagtccagt acccaccgct gtaccgccaa gagccagttc cgctacgtga
    18781 ggcaggctgt attcgatatg tttgagatta tgctcgagca tcgctaccca gccggaaatc
    18841 tcctgcccta gtgttagcgg cgtggcgtcc tgcaagtggg ttcgaccgat tttgacgata
    18901 tcggcaaatg cacgcgattt ttcactcagt gtctgtgtca gggttttaag ctgcggaatg
    18961 agttgcttgc gcagcgccag cagcgccgca acgtgcatcg ccgtcggaaa gacatcgttg
    19021 gaactttggc ttttgttcac gtcgtcgtta gggtgaactt tacgttccat cccgcgcacg
    19081 ccgccgagta attcactggc ccggttagcc agcacttcgt tcatgttcat gttactttgc
    19141 gtgccggagc cggtctgcca gatagccagc gggaattcgt cgtcatgctg tcctgccagt
    19201 acttcatccg ccgcctgccg aatggcgctc gctttctctt cagacaacaa gcctaaatct
    19261 tcattaactt ttgccgctgc gcgcttggtt agcgccagcg catgaatcag tgaggtgggc
    19321 attttctccg tcgaaatgcg gaaatgctcc agcgagcgtt gagtttgtgc gccccacagc
    19381 ttatctgccg ggacatcaat cgcccccatc gaatcttttt cgctgcgtac tgtattcatg
    19441 gcctgctcct cacctgttta attttttctt tctgttttgc tttcgttaag caacttttag
    19501 ctcacttatt atttactatt tgataacaaa tgtttggtct ttcgtgccat gtaaaaaaac
    19561 cgccccgaag ggcggctctg tttatttcac acagcgagtg cactgtgtga gttgtatctg
    19621 ctggaagaag tcatttcctt tatcatccac aaggataaac gccgggaaat cttccacttc
    19681 aattttccag atggcttcca ttcccagttc cggatattca acacattcca ggctcttaat
    19741 acttccctgc gccaatacag cggccggacc accgatactg ccaaggtaga agccgccgtg
    19801 ttttttacag gcatccgtca cctgctggct gcggttgcct ttcgccagca tgatcatact
    19861 tccgccctgc gcttgcagtt gatcgacata agaatccatc cgtccggcgg tcgttgggcc
    19921 aagagaaccg gaggcataac cttccggcgt tttggccgga cccgcgtagt aaatcggatg
    19981 atctttaatg tactgcggca gcccttcacc gttatccatc cgctctttca gtttggcgtg
    20041 agcaatatca cgaccgacga taatcgtgcc gttaagcgat aagcgtgtag aaacgggata
    20101 ctgcgacaac tgtgcgagga tctctttcat cggacggtta aggtcaacgc gcaccgcttc
    20161 gccttctccc gctttacgca gctcttcagg gatatattta cctggattat gttccagttt
    20221 ttcgatccag atcccctgac ggttgatctt cgctttgata ttacggtcag cagagcagga
    20281 aacacccata ccaaccggac aggatgcgcc gtgacgtggc aggcgaatca cgcgtatgtc
    20341 gtgagcgaag tatttaccac caaactgcgc acccagaccg agattttgcg cttcgatcag
    20401 caattctttt tccagttcca catcgcggaa tgcctgaccg tgctcattcc cttccgttgg
    20461 cagttcgtca tagtatttag cggaagccag tttcactgtt ttaaggttag tttctgcaga
    20521 agttccacca attacgaacg caatatgata cggaggacag gccgccgtac ccagcgtgcg
    20581 catcttctca accaggtaat ttttcagttt ccccggcgtt agtaacgctt tggtttcctg
    20641 atagagatac gtcttgtttg ccgaaccacc acctttggcg atacagagga atttgtactc
    20701 gtcgccatca acggcataaa gatcgatctg cgctggcaga ttggtgccgg tattcacctc
    20761 tttatacata tccagcggcg cgttttgcga gtagcgcaaa ttatcttcga tataagtgtt
    20821 atagacaccg cgcgccagcg ccgcttcatc acagccaccg gtccatacac gctgcccttt
    20881 tttaccaaca ataatcgcag tgccggtatc ctgacaggtt ggcagaacgc ctttcgccgc
    20941 gatgtcggag ttacgcagga attgcagcgc cacatattta tcattttcgc tggcctccgg
    21001 gtcacgcaga atgtcggcca cctgttgttg gtgagccgga cgcagcataa acgacgcatc
    21061 atgaaacgcc tgacgcgcca acagagttaa cgcttcgggt gcgactttca aaatctcctg
    21121 cccttcaaat tcagatacgc taacgtgttc gctggttagc aggtaatact cagtatcatc
    21181 ttttttgagt ggaaaaggag cctgataatg aaagggtttg tttgacattg ttctctcact
    21241 tactgcctgg tttggttatg ctctgggcgg gtgttccgtt gccctgttaa aagcgagtaa
    21301 caatatccta cacacttttt taacaaaaac tgagactagt acgacttttt gcggctccag
    21361 gttacttccc gtaggattct tgctttaata gtgggattaa tttccacatt aaaacaggga
    21421 ttgatcatgc aaaaactcat taactcagtg caaaactatg cctggggcag caaaacggcg
    21481 ttgactgaac tttatggtat ggaaaatccg tccagccagc cgatggccga gctgtggatg
    21541 ggcgcacatc cgaaaagcag ttcacgagtg cagaatgccg ccggagatat cgtttcactg
    21601 cgtgatgtga ttgagagtga taaatcgact ctgctcggag aggccgttgc caaacgcttt
    21661 ggcgaactgc ctttcctgtt caaagtatta tgcgcagcac agccgctctc cattcaggtt
    21721 catccaaaca aacgcaattc tgaaatcggt tttgccaaag aaaatgccgc aggtatcccg
    21781 atggatgccg ccgagcgtaa ctataaagat cctaaccaca agccggagct ggtttttgcg
    21841 ctgacgcctt tccttgcgat gaacgcgttt cgtgagtttt ccgagattgt ctccctactc
    21901 cagccagtcg caggtgcaca tccggcaatt gctcactttt tacaacagcc taatgccgaa
    21961 cgtttaagcg aactgttcgc cagcctgttg aatatgcagg gtgaagaaaa atcccgcgcg
    22021 ctggcgattt taaaatcggc gctcgacagt cagcagggtg aaccgtggca aacgattcgt
    22081 ttaatttctg aattttaccc ggaagacagc ggcctgttct ccccgctgtt actgaatgtg
    22141 gtgaaattga atcctggcga agcgatgttc ctgttcgctg aaacaccgca cgcttacctg
    22201 caaggcgtgg cgctggaagt gatggcgaac tccgataacg tgctgcgtgc gggtctgacg
    22261 cctaagtaca ttgatattcc ggaactggtt gccaatgtga aattcgaagc caaaccggct
    22321 aaccagttgt tgacccagcc ggtgaaacaa ggtgcagaac tggacttccc gattccagtg
    22381 gatgattttg ccttctcgct gcatgacctt agtgataaag aaaccaccat tagccagcag
    22441 agtgccgcca ttttgttctg cgtcgaaggc gatgcaacgt tgtggaaagg ttctcagcag
    22501 ttacagctta aaccgggtga atcagcgttt attgccgcca acgaatcacc ggtgactgtc
    22561 aaaggccacg gccgtttagc gcgtgtttac aacaagctgt aagagcttac tgaaaaaatt
    22621 aacatctctt gctaagctta ttaaaggctt ataacacctt caggcggcca gtccgcctga
    22681 tttcatttta tggataatca ttatgaataa atcgctggta gcggtaggcg tcattgttgc
    22741 gctaggcgta gtctggacag gcggcgcatg gtatacaggc aagaagattg aaacccatct
    22801 cgaagacatg gtcgcgcagg cgaacgcgca actcaaactg acagctcctg aatccaacct
    22861 ggaagtgagt tatcaaaact atcatcgcgg cgtattcagc agccagttgc aactgttggt
    22921 gaaacccatt gccgggaaag aaaatccgtg gattaaaagc ggtcagagcg tcatcttcaa
    22981 cgaatcggtt gatcatggtc ccttcccgct tgcccagctt aaaaaactga acctgatccc
    23041 gtcgatggca tcaattcaaa ccacgctggt taataacgaa gtaagcaaac cactgtttga
    23101 tatggcaaaa ggtgaaacgc cttttgagat taactcgcgc attggttaca gcggtgattc
    23161 cagttccgat atttcgctca agccactgaa ttacgagcaa aaggatgaaa aagtcgcctt
    23221 tagcggcggc gagttccagt taaatgctga cagagacggc aaagccatct ccctttccgg
    23281 ggaggcgcaa agtggtcgga tagacgcagt taacgaatac aaccagaaag tgcagttgac
    23341 ctttaataat ctgaaaaccg acggttccag cacgctggca agttttggtg agcgcgtagg
    23401 aaaccaaaaa ctgtcactgg aaaaaatgac catttcagtg gaaggcaaag aactggcact
    23461 gctggaaggc atggagatca gcggtaaatc ggatctggtc aatgacggta aaacgatcaa
    23521 tagccaactg gattactcgc taaacagcct gaaggtacag aatcaggatc tgggcagcgg
    23581 caagctgact ttaaaagtcg gccagattga tggtgaagcc tggcatcagt ttagccagca
    23641 atataacgcg caaactcagg cgctgctggc acagccagaa attgccaaca atcccgaact
    23701 ttatcaggag aaagtgacgg aagccttctt tagcgccctg ccgctgatgt tgaaaggcga
    23761 tccggtgatt actatcgcgc cgctaagctg gaaaaacagt cagggtgaaa gtgcgctgaa
    23821 tctgtcgctg ttcctgaaag atccggcaac gactaaagaa gcgccgcaaa cgctggcgca
    23881 ggaagtagat cgttcggtta aatctctgga tgcgaaactg accattccgg tggatatggc
    23941 aactgagttt atgactcagg tagcgaagct ggaaggttat caggaagatc aagcgaaaaa
    24001 actggcgaaa cagcaagttg aaggtgcatc agcaatgggg cagatgttcc gtctgaccac
    24061 cttgcaggac aataccatca ccaccagcct gcaatatact aacggtcaga taacgttaaa
    24121 cgggcagaaa atgccactgg aagatttcgt tggtatgttt gcaatgccgg cattaaatgt
    24181 tccggtcgta cccgctattc cgcagcagta attcacagac aaaacgccgg agatttttct
    24241 ctccggcgtt attttttact tcagcataaa gtcatactga atactcattt tcacatccca
    24301 gtaattcagg cgatcgccca gcgtactgcc atcgcgcttt tcccgccctt tatcggcagc
    24361 aagaaagaaa ttcatccctt gtaacggccc ttcgctgacc cgatagttca taccacctaa
    24421 gatacccgtg ccttcaatcc gcacatctcg tttactggat tcatagccgc gagtcacaat
    24481 cggtgcaaat gtcagcgtaa attgcggcgt taaacgatag ttgacgccca attgccagga
    24541 ctgcattcct tcacggttac gatcaagact taatgaaaag ggatagccaa tgtcggtatc
    24601 caccacggca ccattcattg catggcgcaa ataaccacca ctgccaaata cgcttcccca
    24661 cggcgcattc caggtcaatt gtccactaac caacgccgtt tcattgggct ggctggtatt
    24721 acgacttaaa ccttcgagct gcgcgtaaaa gccgagcaac tcccctttca acgtataatt
    24781 cgggtcgagc ggtatgcttt tctcgagaat actttgtatc tgattggaaa catctttctg
    24841 taccgatgcc cccagagaat agcgactggt atcatcatgc caactggcgg ctaaaaaaga
    24901 acgtgcgcga tgtttatcgt attttcccgt ctggctgtta tacacccctt catcacgaat
    24961 accggttgtt gaatcagtcc ataaaaatac tcgtgaacgc cacattcccc acagatcaat
    25021 atttggtgtt aaagcccaat aacctaatgc tccttcagaa actgtcggta atgtacgttg
    25081 cgaactgcca gccattaacg gaatacgata aggtaagcta ccataactat aatccgtccc
    25141 gaagcgacct agtttaagca ggctattttc actgggtttt atttttactg cccccagggc
    25201 aaagccaaaa cttttatcac catcaagata ccaccgggta ctcatatcag cacgagcacc
    25261 taatttataa acataatatg ccccgccttc aacgccaata atattggaat aataaccact
    25321 attgaaatcg agcaatcctc cctgtaccca tgcgtaaata tctcttcccg cgccagcact
    25381 gggcttatcc gccctgcgca attcgttttt cagtctgatg cgtagtcccg cttcatcgtc
    25441 agccagttgc gccgcataag cagaggtaag gccagaggca gccgtcaggc aaataacggc
    25501 catggccact attttt
//
LOCUS       DAZKHF020000040        25446 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2201S31241
            SAMN43267800-rid25106413.denovo.040, whole genome shotgun sequence.
ACCESSION   DAZKHF020000040 DAZKHF020000000
VERSION     DAZKHF020000040.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267800
            Sequence Read Archive: SRR30307263
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 25446)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25446)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 25446)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350581.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350581.2
            Genome Coverage       :: 151x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:21:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,652
            CDSs (total)                      :: 5,557
            Genes (coding)                    :: 5,320
            CDSs (with protein)               :: 5,320
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 237
            CDSs (without protein)            :: 237
            Pseudo Genes (ambiguous residues) :: 0 of 237
            Pseudo Genes (frameshifted)       :: 102 of 237
            Pseudo Genes (incomplete)         :: 130 of 237
            Pseudo Genes (internal stop)      :: 52 of 237
            Pseudo Genes (multiple problems)  :: 41 of 237
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25446
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267800-rid25106413.denovo.040"
                     /serotype="O 26:H11"
                     /isolate="2201S31241"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-01"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            81..1373
                     /locus_tag="ACHOSC_RS06805"
                     /old_locus_tag="pgaptmp_001361"
     CDS             81..1373
                     /locus_tag="ACHOSC_RS06805"
                     /old_locus_tag="pgaptmp_001361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HGU4547335.1"
                     /translation="MIKSKIVLLSALVSCALISGCKEENKTNVSIEFMHSSVEQERQA
                     VISKLIARFEKENPGITVKQVPVEEDAYNTKVITLSRSGSLPEVIETSHDYAKVMDKE
                     QLIDRKAVATVISNVGEGAFYDGVLRIVRTEDGSAWTGVPVSAWIGGIWYRKDVLAKA
                     GLEEPKNWQQLLDVAQKLNDPANKKYGIALPTAESVLTEQSFSQFALSNQANVFNAEG
                     KITLDTPEMMQALTYYRDLAANTMPGSNDIMEVKDAFMNGTAPMAIYSTYILPAVIKE
                     GDPKNVGFVVPTEKNSAVYGMLTSLTITAGQKTEETEAAEKFVTFMEQADNIADWVMM
                     SPGAALPVNKAVVTTATWKDNDVIKALGELPNQLIGELPNIQVFGAVGDKNFTRMGDV
                     TGSGVVSSMVHNVTVGKADLPGTLQASQKKLDELIEQH"
     gene            1394..2275
                     /locus_tag="ACHOSC_RS06810"
                     /old_locus_tag="pgaptmp_001362"
     CDS             1394..2275
                     /locus_tag="ACHOSC_RS06810"
                     /old_locus_tag="pgaptmp_001362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HGU4547336.1"
                     /translation="MNRLFSGRSDMPFALLLLAPSLLLLGGLVAWPMVSNIEISFLRL
                     PLNPNIESTFVGVSNYVRILSDPGFWHSLWMTVWYTALVVAGSTVLGLAVAMFFNREF
                     RLRKTARSLVILSYVTPSISLVFAWKYMFNNGYGIVNYLGVDLLHLYEQAPLWFDNPG
                     SSFVLVVLFAIWRYFPYAFISFLAILQTIDKSLYEAAEMDGANAWQRFRIVTLPAIMP
                     VLATVVTLRTIWMFYMFADVYLLTTKVDILGVYLYKTAFAFNDLGKAAAISVVLFIII
                     FAVILLTRKRVNLNGNK"
     gene            2262..3104
                     /locus_tag="ACHOSC_RS06815"
                     /old_locus_tag="pgaptmp_001363"
     CDS             2262..3104
                     /locus_tag="ACHOSC_RS06815"
                     /old_locus_tag="pgaptmp_001363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HGU4547337.1"
                     /translation="MATNKRTLSRIGFYCGLALFLIITLFPFFVMLMTSFKGAKEAIS
                     LHPTLLPQQWTLEHYVDIFNPMIFPFVDYFRNSLVVSVVSSVVAVFLGILGAYALSRL
                     RFKGRMTINASFYTVYMFSGILLVVPLFKIITALGIYDTEMALIITMVTQTLPTAVFM
                     LKSYFDTIPDEIEEAAMMDGLNRLQIIFRITVPLAMSGLISVFVYCFMVAWNDYLFAS
                     IFLSSASNFTLPVGLNALFSTPDYIWGRMMAASLVTALPVVIMYALSERFIKSGLTAG
                     GVKG"
     gene            3135..4187
                     /locus_tag="ACHOSC_RS06820"
                     /old_locus_tag="pgaptmp_001364"
     CDS             3135..4187
                     /locus_tag="ACHOSC_RS06820"
                     /old_locus_tag="pgaptmp_001364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding alcohol dehydrogenase"
                     /protein_id="HGU4547338.1"
                     /translation="MKKLVATAPRVAALVEYEDRAILANEVKIRVRFGAPKHGTEVVD
                     FRAASPFIDEDFNGEWQMFTPRPADAPRGIEFGKFQLGNMVVGDIIECGSDVTDYAVG
                     DSVCGYGPLSETVIINAVNNYKLRKMPEGSSWKNAVCYDPAQFAMSGVRDANVRVGDF
                     VVVVGLGAIGQIAIQLAKRAGASVVIGVDPIAYRCDIARRHGADFCLNPIGTDVGKEI
                     KTLTGKQGADVIIETSGYADALQSALRGLAYGGTISYVAFAKPFAEGFNLGREAHFNN
                     AKIVFSRACSEPNPDYPRWSRKRIEETCWELLMNGYLNCEDLIDPVVTFAKSPESYMQ
                     YVDQHPEQSIKMGVTF"
     gene            4205..4993
                     /locus_tag="ACHOSC_RS06825"
                     /old_locus_tag="pgaptmp_001365"
     CDS             4205..4993
                     /locus_tag="ACHOSC_RS06825"
                     /old_locus_tag="pgaptmp_001365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415830.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase family
                     protein"
                     /protein_id="HGU4547339.1"
                     /translation="MKIGTQNQAFFPENILEKFRYIKEMGFDGFEIDGKLLVNNLEEV
                     KAAIKETGLPVTTACGGYDGWIGDFIEERRLNGLKQIERILEALAEVGGKGIVVPAAW
                     GMFTFRLPPMTSPRSLDGDRKMVSDSLHVLEQVAARTGTVVYLEPLNRYQDHMINTLA
                     DARRYIVENDLKHVQIIGDFYHMNIEEDNLAQALHDNRDLLGHVHIADNHRYQPGSGT
                     LDFHALFEQLRADNYQGYVVYEGRIRAEEPAQAYRDSLAWLRTC"
     gene            5003..6058
                     /locus_tag="ACHOSC_RS06830"
                     /old_locus_tag="pgaptmp_001366"
     CDS             5003..6058
                     /locus_tag="ACHOSC_RS06830"
                     /old_locus_tag="pgaptmp_001366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HGU4547340.1"
                     /translation="MKSAMTSSPLRVAIIGAGQVADKVHASYYCTRNDLELVAVCDSR
                     LSQAQALADKYGNASVWDDPQAMLLAVKPDVVSVCSPNRFHYEHTLMALEAGCHVMCE
                     KPPAMTPEQAREMCDTARKLGKVLAYDFHHRFALDTQQLREQVTNGVLGEIYVTTARA
                     LRRCGVPGWGVFTNKELQGGGPLIDIGIHMLDAAMYVLGFPAVKSVNAHSFQKIGTQK
                     SCGQFGEWDPATYSVEDSLFGTIEFHNGGILWLETSFALNIREQSIMNVSFCGDKAGA
                     TLFPAHIYTDNNGELMTLMQRELADDNRHLRSMEAFINHVQGKPVMIADAEQGYIIQQ
                     LVAALYQSAETGTRVEL"
     gene            6055..8322
                     /gene="ycjT"
                     /locus_tag="ACHOSC_RS06835"
                     /old_locus_tag="pgaptmp_001367"
     CDS             6055..8322
                     /gene="ycjT"
                     /locus_tag="ACHOSC_RS06835"
                     /old_locus_tag="pgaptmp_001367"
                     /EC_number="2.4.1.230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="kojibiose phosphorylase"
                     /protein_id="HGU4547341.1"
                     /translation="MTRPVTLSEPHFSQHTLNKYASLMAQGNGYLGLRASHEEDYTRQ
                     TRGMYLAGLYHRAGKGEINELVNLPDILGMEIAINGEVFSLSREAWQRELDFASGELR
                     RSVVWRTSNGTGYTIASRRFVSADQLPLIVLEITITPLDADASVLISTGIDATQTNHG
                     RQHLDETQVRVFGQHLMQGIYTTQDGRSDVAISCCCMVSGDVQQCYTAKERRLQQHTS
                     AQLHAGETVTLQKLVWIDWRDDRQAVLDEWGSASLRQLEMCAQQSYDQLLAVSTENWR
                     QWWQKRRITVNGGEAHDQQALDYALYHLRIMTPAHDERSSIAAKGLTGEGYKGHVFWD
                     TEVFLLPFHLFSDPTVARSLLRYRWHNLPGAQEKARRNGWQGALFPWESARSGEEETP
                     EFAAINIRTGLRQKVASAQAEHHLVADIAWAVIQYWQTTGDESFIAHEGMALLLETAK
                     FWISRAVRVNDRLEIHDVIGPDEYTEHVNNNAFTSYMAYYNVQQALSIARQFGCSDDA
                     FIHRAEMFLKELRLPEIQPDGVLPQDDSFMAKPAINLAKYKAAAGKQTILLDYSRAEV
                     NEMQILKQADVVMLNYMLPEQFSAASCLANLQFYEPRTIHDSSLSKAIHGIVAARCGL
                     LTQSYQFWREGTEIDLGADPHSCDDGIHAAATGAIWLGAIQGFAGVSVRDGELHLNPA
                     LPEQWQQLSFPLFWQGCELQVTLDAQRIAIRTSAPVSLRLNGQLISVAEESVFCLGDF
                     ILPFNGTATTHQEDE"
     gene            8319..8978
                     /gene="pgmB"
                     /locus_tag="ACHOSC_RS06840"
                     /old_locus_tag="pgaptmp_001368"
     CDS             8319..8978
                     /gene="pgmB"
                     /locus_tag="ACHOSC_RS06840"
                     /old_locus_tag="pgaptmp_001368"
                     /EC_number="5.4.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008801 - beta-phosphoglucomutase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-phosphoglucomutase"
                     /protein_id="HGU4547342.1"
                     /translation="MKLQGVIFDLDGVITDTAHLHFQAWQQIAAEIGISIDAQFNESL
                     KGISRDESLRRILQHGGKEGDFNSQERAQLAYRKNLLYVHSLRELTVSAVLPGIRSLL
                     ADLRAQQIPVGLASVSLNAPTILAALELREFFTFCADASQLKNSKPDPEIFLAACAGL
                     GVPPQACIGIEDAQAGIDAINASGMRSVGIGAGLTGAQLLLPSTDSLTWSRLSAFWQN
                     V"
     gene            8992..10074
                     /locus_tag="ACHOSC_RS06845"
                     /old_locus_tag="pgaptmp_001369"
     CDS             8992..10074
                     /locus_tag="ACHOSC_RS06845"
                     /old_locus_tag="pgaptmp_001369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HGU4547343.1"
                     /translation="MAQLSLQHIQKIYDNQVHVVKDFNLEIADKEFIVFVGPSGCGKS
                     TTLRMIAGLEEISGGDLLIDGKRMNDVPAKARNIAMVFQNYALYPHMTVYDNMAFGLK
                     MQKIAKEVIDERVNWAAQILGLREYLKRKPGALSGGQRQRVALGRAIVREAGVFLMDE
                     PLSNLDAKLRVQMRAEISKLHQKLNTTMIYVTHDQTEAMTMATRIVIMKDGIVQQVGA
                     PKTVYNQPANMFVAGFIGSPAMNFIRGTIDGDKFVTETLKLTIPEEKLAVLKTQGSLH
                     KPIVMGIRPEDIHPDAQEENNISAKISVAELTGAEFMLYTTVGGHELVVRAGALNDYH
                     AGENITIHFDMTKCHFFDAETEIAIR"
     gene            10119..11024
                     /gene="ompG"
                     /locus_tag="ACHOSC_RS06850"
                     /old_locus_tag="pgaptmp_001370"
     CDS             10119..11024
                     /gene="ompG"
                     /locus_tag="ACHOSC_RS06850"
                     /old_locus_tag="pgaptmp_001370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="monomeric porin OmpG"
                     /protein_id="HGU4547344.1"
                     /translation="MKKLLPCTALVMCAGMACAQAEERNDWHFNIGAMYEIENVEGYG
                     EDMDGLAEPSVYFNAANGPWRIALAYYQEGPVDYSAGKRGTWFDRPELEVHYQFLEND
                     DFSFGLTGGFRNYGYHYVDEPGKDTANMQRWKIAPDWDVKLTDDLRFNGWLSMYKFAN
                     DLNTTGYADTRVETETGLQYTFNETVALRVNYYLERGFNMDDSRNNGEFSTQEIRAYL
                     PLTLGNHSVTPYTRIGLDRWSNWDWQDDIEREGHDFNRVGLFYGYDFQNGLSVSLEYA
                     FEWQDHDEGDSDKFHYAGVGVNYSF"
     gene            complement(11135..12133)
                     /locus_tag="ACHOSC_RS06855"
                     /old_locus_tag="pgaptmp_001371"
     CDS             complement(11135..12133)
                     /locus_tag="ACHOSC_RS06855"
                     /old_locus_tag="pgaptmp_001371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HGU4547345.1"
                     /translation="MSPTIYDIARVAGVSKSTVSRVLNKQTNISPEAREKVLRAIEEL
                     QYQPNKLARALTSSGFDAIMVISTRSTKTTAGNPFFSEVLHAITAKAEEEGFDVILQT
                     SHNPAEDLQKCESKIKQKMIKGIIMLSSPADESFFAQLDKYDIPVVVIGKVEGQYAHV
                     YSVDTDNFGDSIALTDALIESGHQNIACLHAPLDVHVSVDRVNGYKQSLAAHNIAVRD
                     EWIVDGGYTHETALQAARELLSQSPLPEAVFATDSLKLMSIYRAAAEKNIAIPQQLAV
                     VGYSNETLSFILTPAPGGIDIPTQELGQQSCELLFRLISGKPSPQNITVATHMTLK"
     gene            12288..13685
                     /locus_tag="ACHOSC_RS06860"
                     /old_locus_tag="pgaptmp_001372"
     CDS             12288..13685
                     /locus_tag="ACHOSC_RS06860"
                     /old_locus_tag="pgaptmp_001372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcjX family protein"
                     /protein_id="HGU4547346.1"
                     /translation="MKRLKNELNALVNRGVDRHLRLAVTGLSRSGKTAFITAMVNQLL
                     NIHAGARLPLLSAVREERLLGVKRIPQRDFGIPRFTYDEGLAQLYGDPPAWPTPTRGV
                     SEIRLALRFKSNDSLLRHFKDTSTLYLEIVDYPGEWLLDLPMLAQDYLSWSRQMTGLL
                     NGQRGEWSVKWRMMCEGLDPLAPADENRLADIAAAWTDYLHHCKQQGLHFIQPGRFVL
                     PGDMAGAPALQFFPWPDVDAWGESKLAQADKHTNAGMLRERFNYYCEKVVKGFYKNHF
                     LRFDRQIVLVDCLQPLNSGPQAFNDMRLALTQLMQSFHYGQRTLFRRLFSPVIDKLLF
                     AATKADHVTIDQHANMVSLLQQLIQDAWQNAAFEGISMDCLGLASVQATTSGIIDVNG
                     EKIPALRGNRLSDGAPLTVYPGEVPARLPGQAFWDKQGFQFEAFRPQVMDVDKPLPHI
                     RLDAALEFLIGDKLR"
     gene            13682..14743
                     /locus_tag="ACHOSC_RS06865"
                     /old_locus_tag="pgaptmp_001373"
     CDS             13682..14743
                     /locus_tag="ACHOSC_RS06865"
                     /old_locus_tag="pgaptmp_001373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcjF family protein"
                     /protein_id="HGU4547347.1"
                     /translation="MTEPLKPRIDFDGPLEVDQNPKFRAQQTFDENQAQNFAPATLDE
                     APEEEGQVEAVMDAALRPKRSLWRKMVMGGLALFGASVVGQGVQWTMNAWQTQDWVAL
                     GGCAAGALIIGAGVGSVVTEWRRLWRLRQRAHERDEARDLLHSHGTGKGRAFCEKLAQ
                     QAGIDQSHPALQRWYASIHETQNDREVVSLYAHLVQPVLDAQARREISRSAAESTLMI
                     AVSPLALVDMAFIAWRNLRLINRIATLYGIELGYYSRLRLFKLVLLNIAFAGASELVR
                     EVGMDWMSQDLAARLSTRAAQGIGAGLLTARLGIKAMELCRPLPWIDDDKPRLGDFRR
                     QLIGQVKETLQKGKTPSEK"
     gene            14891..16432
                     /gene="tyrR"
                     /locus_tag="ACHOSC_RS06870"
                     /old_locus_tag="pgaptmp_001374"
     CDS             14891..16432
                     /gene="tyrR"
                     /locus_tag="ACHOSC_RS06870"
                     /old_locus_tag="pgaptmp_001374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator TyrR"
                     /protein_id="HGU4547348.1"
                     /translation="MRLEVFCEDRLGLTRELLDLLVLRGIDLRGIEIDPIGRIYLNFA
                     ELEFESFSSLMAEIRRIAGVTDVRTVPWMPSEREHLALSALLEALPEPVLSVDMKSKV
                     DMANPASCQLFAQKLDRLRNHTAAQLINGFNFLRWLESEPQDSHNEHVVINGQNFLME
                     ITPVYLQDENDQHVLTGAVVMLRSTIRMGRQLQNVAAQDVSAFSQIVAVSPKMKHVVE
                     QAQKLAMLSAPLLITGDTGTGKDLFAYACHQASPRAGKPYLALNCASIPEDAVESELF
                     GHAPEGKKGFFEQANGGSVLLDEIGEMSPRMQAKLLRFLNDGTFRRVGEDHEVHVDVR
                     VICATQKNLVELVQKGMFREDLYYRLNVLTLNLPPLRDCPQDIMPLTELFVARFADEQ
                     GVPRPKLAADLNTVLTRYAWPGNVRQLKNAIYRALTQLDGYELRPQDILLPDYDAATV
                     AVGEDAMEGSLDEITSRFERSVLTQLYRNYPSTRKLAKRLGVSHTAIANKLREYGLSQ
                     KKNEE"
     gene            complement(16476..16982)
                     /gene="tpx"
                     /locus_tag="ACHOSC_RS06875"
                     /old_locus_tag="pgaptmp_001375"
     CDS             complement(16476..16982)
                     /gene="tpx"
                     /locus_tag="ACHOSC_RS06875"
                     /old_locus_tag="pgaptmp_001375"
                     /EC_number="1.11.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008379 - thioredoxin peroxidase activity
                     [Evidence IEA];
                     GO_function: GO:0016684 - oxidoreductase activity, acting
                     on peroxide as acceptor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiol peroxidase"
                     /protein_id="HGU4547349.1"
                     /translation="MSQTVHFQGNPVTVANSIPQAGSKAQTFTLVAKDLSDVTLGQFA
                     GKRKVLNIFPSIDTGVCAASVRKFNQLATEIDNTVVLCISADLPFAQSRFCGAEGLNN
                     VITLSTFRNAEFLQAFGVAIADGPLKGLAARAVVVIDENDNVIFSQLVDEITTEPDYE
                     AALAVLKA"
     gene            17101..18066
                     /gene="ycjG"
                     /locus_tag="ACHOSC_RS06880"
                     /old_locus_tag="pgaptmp_001376"
     CDS             17101..18066
                     /gene="ycjG"
                     /locus_tag="ACHOSC_RS06880"
                     /old_locus_tag="pgaptmp_001376"
                     /EC_number="5.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707230.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016855 - racemase and epimerase activity,
                     acting on amino acids and derivatives [Evidence IEA];
                     GO_process: GO:0009063 - amino acid catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-Ala-D/L-Glu epimerase"
                     /protein_id="HGU4547350.1"
                     /translation="MRTVKVFEEAWPLHTPFVIARGSRSEARVVVVELEEEGIKGTGE
                     CTPYPRYGESDASVMAQIMSVVPQLEKGLTREELQKILPAGAARNALDCALWDLAARK
                     QQQSLADLIGITLPGTVTTAQTVVIGTPDQMANSASTLWQAGAKLLKVKLDNHLISER
                     MVAIRTAVPDATLIVDANESWRAEGLAARCQLLADLGVAMLEQPLPAQDDAALENFIH
                     PLPICADESCHTRSNLKALKGRYEMVNIKLDKTGGLTEALALATEARAQGFRLMLGCM
                     LCTSRAISAALPLVPQVSFVDLDGPTWLAVDVEPALQFTTGELHL"
     gene            complement(18041..18769)
                     /gene="mpaA"
                     /locus_tag="ACHOSC_RS06885"
                     /old_locus_tag="pgaptmp_001377"
     CDS             complement(18041..18769)
                     /gene="mpaA"
                     /locus_tag="ACHOSC_RS06885"
                     /old_locus_tag="pgaptmp_001377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415842.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004181 - metallocarboxypeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="murein tripeptide amidase MpaA"
                     /protein_id="HGU4547351.1"
                     /translation="MTVNRPRAERGAFPPGTEHYGRSLLGAPLIWFPATAASRESGLI
                     LAGTHGDENSSVVTLSCALRTLTPSLRRHHVVLCVNPDGCQLGLRANANGVDLNRNFP
                     AANWKEGETVYRWNSAAEERDVVLLTGDKPGSEPETQALCQLIHRIQPAWVVSFHDPL
                     ACIEDPRHSELGEWLAQAFELPLVTSVGYETPGSFGSWCADLNLHCITAEFPPISSDE
                     ASEKYLFAMANLLRWHPKDAIRPS"
     gene            complement(19078..19716)
                     /locus_tag="ACHOSC_RS06890"
                     /old_locus_tag="pgaptmp_001378"
     CDS             complement(19078..19716)
                     /locus_tag="ACHOSC_RS06890"
                     /old_locus_tag="pgaptmp_001378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309933.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HGU4547352.1"
                     /translation="MKNVLILGAGGQIARHVINQLADKQTIKQTLFARQPAKIHKPYP
                     TNSKIIMGDVLNHAALKQAMQGQDVVYANLTGEDLDIQANSVIAAMKACDVKRLIFVL
                     SLGIYDEVPGKFGEWNNAVIGEPLKPFRRAADAIEASGLEYTILRPAWLTDEDIIDYE
                     LTSRNEPFKGTIVSRKSVAALITDIIDKPEKHIGENIGINQPVTDGDKPLFM"
     gene            complement(19734..20666)
                     /locus_tag="ACHOSC_RS06895"
                     /old_locus_tag="pgaptmp_001379"
     CDS             complement(19734..20666)
                     /locus_tag="ACHOSC_RS06895"
                     /old_locus_tag="pgaptmp_001379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000680621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HGU4547353.1"
                     /translation="MKHIQIRNSDMAWHIAANIQFPPNFDESKQYPAIISVHPFGSCK
                     EQTSGNIYGKALAEKGYLVLAYDASFQGESGGEPRWIEDPTQRVEDISRVIDYAVTLP
                     YVNAERIGVLGVCGGGGYAINAALTEKRIKAVVSITGVNIGRLFREGFSNYDPIGALN
                     AMASQRTKEARGGELQINELLPASLDAAKAHGLTERDVYEATDYYKTPRGQQPGGATK
                     MLFSHAQKTLAWDAFAFTEVLLTQPVMVVVGEKVGAFGAYRDGLEVYGRAMVSQDRQL
                     VSLPDFSHYELYDKPEAVQKALEKVIPFFNTHLG"
     gene            complement(20666..21586)
                     /locus_tag="ACHOSC_RS06900"
                     /old_locus_tag="pgaptmp_001380"
     CDS             complement(20666..21586)
                     /locus_tag="ACHOSC_RS06900"
                     /old_locus_tag="pgaptmp_001380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415843.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HGU4547354.1"
                     /translation="MMNNKVSFTNSNNPTISLSAVSYFPPKFDETRQYQAIVVSHPGG
                     GVKEQTAGTYAKKLAEKGFVTIAYDASYQGESGGEPRQLENPYIRTEDISAVIDYLTT
                     LSYVDNTRIGAMGICAGAGYTANAAIQDRRIKAIGTVSAVNIGSIFRNGWENNVKSID
                     ALPYVEAGSNARTSDISSGEYAIMPLAPMKESDAPNEELRQAWEYYHTPRAQYPTAPG
                     YATLRSLNQIITYDAYHMAEVYLTQPTQIVAGSQAGSKWMSDDLYDRASSQDKRYHIV
                     EGANHMDLYDGKAYVAEAISVLAPFFEETL"
     gene            21724..22623
                     /gene="pgrR"
                     /locus_tag="ACHOSC_RS06905"
                     /old_locus_tag="pgaptmp_001381"
     CDS             21724..22623
                     /gene="pgrR"
                     /locus_tag="ACHOSC_RS06905"
                     /old_locus_tag="pgaptmp_001381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator PgrR"
                     /protein_id="HGU4547355.1"
                     /translation="MKREEIADLMAFVVVAEERSFTRAAARLSMAQSALSQIVRRIEE
                     RLGLRLLTRTTRSVVPTEAGEHLLSVLGPMLHDIDSALASLSELQNRPSGTIRITTVE
                     HAAKTILLPAMRTFLKSHPEIDIQLTIDYGLTDVVSERFDAGVRLGGEMDKDMIAIRI
                     GPDIPMAIVGSPDYFSRRSVPTSVSQLIDHQAINLYLPTSGTANRWRLIRGGREVRVR
                     MEGQLLLNTIDLIIDAAIDGHGLAYLPYDQVERAIKEKKLIRVLDKFTPDLPGHHLYY
                     PHRRHAGSAFSLFIDRLKYKGAV"
     gene            22960..24573
                     /gene="mppA"
                     /locus_tag="ACHOSC_RS06910"
                     /old_locus_tag="pgaptmp_001382"
     CDS             22960..24573
                     /gene="mppA"
                     /locus_tag="ACHOSC_RS06910"
                     /old_locus_tag="pgaptmp_001382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415845.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein tripeptide ABC transporter
                     substrate-binding protein MppA"
                     /protein_id="HGU4547356.1"
                     /translation="MKHSVSVTCCALLVSSISLSYAAEVPSGTVLAEKQELVRHIKDE
                     PASLDPAKAVGLPEIQVIRDLFEGLVNQNEKGEIVPGVATQWKSNDNRIWTFTLRDNA
                     KWADGTPVTAQDFVYSWQRLVDPKTLSPFAWFAALAGINNAQAIIDGKATPDQLGVTA
                     VDAHTLKIQLDKPLPWFVNLTANFAFFPVQKANVESGKEWTKPGNLIGNGAYVLKDRV
                     VNEKLVVVPNTHYWDNAKTVLQKVTFLPINQESAATKRYLAGDIDITESFPKNMYQKL
                     LKDIPGQVYTPPQLGTYYYAFNTQKGPTADQRVRLALSMTIDRRLMTEKVLGTGEKPA
                     WHFTPDVTAGFTPEPSPFEQMSQEELNAQAKTLLSAAGYGPQKPLKLTLLYNTSENHQ
                     KIAIAVASMWKKNLGVDVKLQNQEWKTYIDSRNTGNFDVIRASWVGDYNEPSTFLTLL
                     TSTHSGNISRFNNPAYDKVLAQASMENTVKARNADYNAAEKILMEQAPIAPIYQYTNG
                     RLIKPWLKGYPINNPEDVAYSRTMYIVKH"
     gene            complement(24624..>25446)
                     /gene="ynaI"
                     /locus_tag="ACHOSC_RS06915"
                     /old_locus_tag="pgaptmp_001383"
     CDS             complement(24624..>25446)
                     /gene="ynaI"
                     /locus_tag="ACHOSC_RS06915"
                     /old_locus_tag="pgaptmp_001383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="low conductance mechanosensitive channel YnaI"
                     /protein_id="HGU4547357.1"
                     /translation="KFITPGVIDFICTSLIAVILTIKLFLLINQFEKQQIKKGRDITS
                     ARIMSRIIKITIIVVLVLLYGEHFGMSLSGLLTFGGIGGLAVGMAGKDILSNFFSGIM
                     LYFDRPFSIGDWIRSPDRNIEGTVAEIGWRITKITTFDNRPLYVPNSLFSSISVENPG
                     RMTNRRITTTIGLRYEDAAKVGVIVEAVREMLKNHPAIDQRQTLLVYFNQFADSSLNI
                     MVYCFTKTTVWAEWLAAQQDVYLKIIDIVQSHGADFAFPSQTLYMDNITPPEQGR"
BASE COUNT         6230 a         6137 c         6771 g         6308 t
ORIGIN      
        1 tagtgaagtt gatatattgg gtaatgaaat aacgctgcgc ccctggcagg ttatgtggat
       61 taaataaaaa ggaacatctc atgattaaat caaaaatcgt gctgttatca gcactggttt
      121 cctgcgccct gatttcaggg tgtaaagaag aaaataaaac gaatgtatcc atcgaattta
      181 tgcattcttc ggtggagcag gagcgccagg ccgttatcag taaattgatt gcccgttttg
      241 aaaaagaaaa ccctggcatc accgttaagc aagtgcccgt ggaagaagat gcctataaca
      301 ctaaagtcat tactctttca cgtagcggtt cgctgccgga agtgatagaa accagccatg
      361 actacgccaa agtgatggac aaagagcagc ttatcgatcg caaagcggtt gccacagtca
      421 tcagcaacgt tggtgaaggc gcgttttacg atggcgtact gcgtattgtg cgtaccgaag
      481 atggtagcgc atggaccggt gttcctgtca gcgcctggat tggcggtatc tggtatcgca
      541 aagatgtgct ggcaaaagcg gggcttgagg agccgaaaaa ctggcaacag ctgctggacg
      601 ttgcacagaa actgaatgac ccggcgaata aaaaatacgg cattgcgctg cctacagcag
      661 aaagcgtgtt gacggaacaa tccttctccc agtttgcgtt atccaaccag gctaacgtct
      721 ttaacgccga aggcaaaatc acccttgata caccagagat gatgcaggca ctgacctatt
      781 accgcgacct tgctgccaac actatgccgg gttctaacga catcatggaa gtgaaagacg
      841 cctttatgaa cggcaccgcg ccgatggcga tttactccac ctatatcctt ccggctgtga
      901 ttaaagaagg cgacccgaaa aacgtcggtt tcgtggtgcc aaccgagaaa aactctgcgg
      961 tctacggcat gttgacctcg ctgaccatta ccgccgggca aaagaccgaa gagacggaag
     1021 cagcagaaaa atttgtcacc tttatggagc aggcagacaa cattgccgac tgggtgatga
     1081 tgtcgccagg tgctgcgctg ccggtgaata aagcggttgt cactaccgcc acctggaaag
     1141 acaacgacgt tattaaggcg ctgggtgaac taccgaatca gctaatcggt gaactgccaa
     1201 atattcaggt ctttggtgca gtaggggata aaaactttac ccgcatgggt gatgtgacgg
     1261 ggtctggcgt ggtgagctcc atggtgcata acgtcaccgt gggtaaagcc gatctccctg
     1321 gcacgctgca agcgagccag aaaaagctgg atgaactgat cgaacagcac taaacccagg
     1381 acaggaatcc gcaatgaaca ggcttttttc aggtcgttcc gatatgccct ttgcgctgct
     1441 gcttctcgcg cccagcttat tactgctggg tggtctggtg gcctggccaa tggtgtcgaa
     1501 tatcgaaatc agttttttac gtctgccgct caatcccaac atcgagtcaa cctttgttgg
     1561 ggtgagtaac tatgtgcgta tcctctccga tcccggcttc tggcactcgc tgtggatgac
     1621 ggtctggtat accgcgctgg tggtggcggg cagcaccgtg ctcgggctgg cagtggcgat
     1681 gtttttcaac cgcgagttcc gtctgcgcaa aaccgcgcgt tcgctggtga tcctctccta
     1741 cgtaacgccg tccatttcgc tggtgtttgc ctggaaatac atgttcaaca acggctacgg
     1801 cattgtgaac tacctcggcg tcgatcttct gcatctctat gagcaggcac cgctgtggtt
     1861 cgataatccg ggcagtagct ttgtgctggt ggtgctgttc gccatctggc gctacttccc
     1921 gtatgccttt atctcgtttc tggcgatttt gcagaccatc gacaaatcgc tgtacgaagc
     1981 ggcagagatg gatggcgcta atgcctggca acggtttcgt atcgtcacgc tgcccgcgat
     2041 tatgccggtc ctggcgacgg tggtgaccct gcgcaccatc tggatgttct acatgttcgc
     2101 ggatgtttat ttgctgacga ccaaggtcga tattctcggt gtatatctct acaaaaccgc
     2161 ctttgccttt aatgatttag gaaaagcggc ggcgatctcg gtggtgctct tcatcattat
     2221 tttcgctgtc attctgctga ccaggaaacg ggtgaacctc aatggcaaca aataaacgca
     2281 cactcagtcg catcggtttt tactgcgggc tggcgctgtt tctcatcatc acgctgtttc
     2341 cattttttgt gatgctgatg acctcgttca agggcgcgaa agaggcgatc tcactgcatc
     2401 ctacgctgct gccgcagcag tggacgctgg agcattacgt cgacattttt aacccgatga
     2461 ttttcccgtt tgtcgactac ttccgtaaca gtctggtggt gtcggtggtt tcatccgtgg
     2521 tggcggtatt cctcggcatt cttggggctt acgcgctttc ccgcctgcgc tttaaaggtc
     2581 ggatgaccat caatgccagc ttttacacgg tgtatatgtt ctctggcatt ttgctggtgg
     2641 tgccgctgtt caaaatcatc accgcgctgg gcatttacga caccgagatg gcgctgatca
     2701 tcaccatggt gacgcaaact ctgcccaccg ccgtgtttat gctgaaaagc tacttcgaca
     2761 ccatccccga tgagatcgaa gaagcggcga tgatggatgg cctcaaccgt ctgcaaatta
     2821 tcttccgcat caccgtgccg ttggcgatgt ccgggctgat atccgtgttc gtctactgct
     2881 ttatggtggc gtggaacgac tatctgtttg cgtcgatttt cctctccagc gccagcaatt
     2941 tcaccttacc ggtgggcctg aacgcgctgt tcagtacgcc agattacatc tggggacgga
     3001 tgatggcggc ctcactggtg accgcattgc cggtggtgat tatgtatgcg ctttcggaac
     3061 gttttattaa gagtggtttg accgccggtg gcgtgaaggg ctaaagcggc cagtttcttt
     3121 acaaggagtt ttaaatgaaa aagttagtag ccacagcacc gcgtgttgct gcgctggttg
     3181 agtatgaaga tcgggcgatt ttagccaatg aagtgaaaat ccgcgtgcgt ttcggcgcac
     3241 cgaaacacgg aacggaagtg gtcgacttcc gcgccgccag cccgtttatc gatgaagact
     3301 ttaacggcga atggcagatg ttcactccgc gtccggcaga tgcgccgcgc ggcattgagt
     3361 ttggcaaatt ccagcttggc aacatggtgg ttggcgacat tatcgagtgc ggcagcgacg
     3421 ttaccgacta cgcggtgggc gacagcgtat gcggctacgg cccgctctcc gagacggtca
     3481 tcattaacgc ggtgaataac tacaagctgc gcaaaatgcc ggaaggcagc tcgtggaaaa
     3541 acgccgtttg ttacgacccg gcgcagtttg ccatgagcgg cgtgcgcgat gccaacgtgc
     3601 gcgtgggtga ttttgtggtg gtggtagggc ttggcgcgat tggtcaaata gccatccaac
     3661 tggcgaaacg cgctggcgcg tcggtggtga ttggcgtcga tcctatcgcc tatcgctgtg
     3721 atattgcccg tcgccacggt gctgatttct gccttaaccc cattggcact gacgtaggaa
     3781 aagagatcaa aacgctgacc ggcaagcagg gtgccgatgt gattatcgaa accagcggtt
     3841 acgccgacgc gctgcaatcg gcgctgcgtg gccttgctta tggcggcacc atctcttatg
     3901 tcgcgtttgc caaaccattt gctgaaggtt ttaacctcgg acgcgaagcg catttcaata
     3961 acgccaaaat tgttttctcc cgtgcgtgca gcgaaccgaa cccggattat ccgcgctgga
     4021 gccgtaagcg tattgaagaa acctgctggg aactgctgat gaacggttat ctcaattgcg
     4081 aagatttaat cgacccggta gtgacctttg ccaagagccc ggaaagctac atgcagtatg
     4141 tcgatcagca tccggaacag agcatcaaaa tgggcgttac gttttaattc aaaggaatga
     4201 aaaaatgaaa ataggcacac agaatcaggc attctttccg gaaaacattc tggagaaatt
     4261 tcgttatatc aaagagatgg gcttcgatgg ttttgagatt gacggcaaac tgctggtcaa
     4321 caatctcgaa gaagtcaaag cagcaatcaa agaaaccggt ttaccggtga ccaccgcctg
     4381 cggtggctat gacggttgga ttggcgactt tatcgaagag cgtcgtctta atggcttaaa
     4441 gcagatcgaa cgcattctcg aagcgctggc agaagtgggg ggtaaaggta tcgtcgttcc
     4501 ggcagcgtgg ggcatgttta ccttccgctt accgccgatg acctcgccgc gtagcctgga
     4561 cggcgaccgc aaaatggtga gtgattccct gcacgtactg gaacaggtcg ccgcgcgtac
     4621 cggaaccgtg gtgtatctcg aaccgttaaa ccgctatcag gatcatatga tcaacaccct
     4681 cgccgatgcc cgccgttaca tcgtcgaaaa cgatcttaaa catgtacaga ttatcggcga
     4741 tttctatcac atgaatatcg aagaagataa cctggcgcag gcgctgcatg acaaccgcga
     4801 cctgctcggt catgtgcata ttgcggataa ccatcgctac cagccgggca gcggcaccct
     4861 ggatttccac gcgctgtttg aacagctgcg cgcggataac tatcagggct atgtagtgta
     4921 cgaagggcgt atccgggcgg aagagcctgc ccaggcgtac cgtgattcgt tggcctggtt
     4981 gcgtacctgc taagaggtct ttgtgaaaag tgcaatgaca agctctccgc tgcgggtcgc
     5041 gataataggc gcaggccagg tggcggataa ggttcatgct tcgtactact gcacccgcaa
     5101 cgatctggaa ctggtggctg tctgtgacag ccgcctttcc caggcgcagg cgctggcaga
     5161 taaatacggt aatgcatccg tgtgggacga tccgcaggcc atgctgctgg cggtgaaacc
     5221 tgatgtggtt agcgtctgct cacctaaccg ttttcattac gaacataccc tgatggcact
     5281 ggaagcgggc tgccatgtga tgtgcgaaaa accgcccgcc atgacgccag aacaggcgcg
     5341 ggaaatgtgc gataccgcgc gcaaactggg caaggtgctg gcctacgact ttcaccaccg
     5401 ttttgcgctc gatacgcaac agctgcgtga acaggtgacc aacggcgttt tgggagagat
     5461 ttacgttacc accgcccgcg ccctgcgtcg ctgcggcgtt cccggctggg gtgtctttac
     5521 caataaagaa ctgcagggtg gtggcccgct tatcgacatc ggcattcata tgctggatgc
     5581 ggcgatgtat gtgctgggct ttccggcggt gaaaagcgtg aatgcgcata gctttcaaaa
     5641 gatcggcacg caaaagagct gcggtcaatt tggcgagtgg gatccggcaa cttacagcgt
     5701 cgaagattcg ttatttggca ccattgaatt tcataacggc ggcattctgt ggctggaaac
     5761 gtcatttgca ctcaacatcc gcgaacagtc gattatgaac gtcagctttt gtggtgataa
     5821 agccggtgcg acgctgtttc cagcacatat ctacaccgat aacaacggtg aattaatgac
     5881 gctgatgcaa cgggaactgg cagacgacaa ccgccatttg cgcagcatgg aagcctttat
     5941 caatcacgta cagggcaagc ccgtgatgat agccgacgcc gagcaggggt acatcatcca
     6001 gcaactggtg gcggcgttgt atcaatccgc agaaacaggg acgcgtgtgg aattatgacc
     6061 aggccagtaa cgttatcaga accccatttc agccagcata ccctgaacaa gtatgcatcg
     6121 ctgatggcgc aggggaacgg ctatcttggg cttcgcgcca gccatgaaga agattacacg
     6181 cgccagacgc gagggatgta tctggcgggg ctgtatcatc gggcgggaaa aggtgaaatc
     6241 aacgaactgg tgaacctgcc tgatatcttg gggatggaga ttgccataaa tggtgaggtt
     6301 ttctcgttat cccgcgaagc ctggcagcgt gaacttgact ttgccagtgg agaattacgc
     6361 cgtagcgttg tctggcgtac cagcaacggc acaggttaca ccatcgccag ccgtcgcttt
     6421 gtttcggcag accaactgcc gctcattgtg ctggaaatca ctattacgcc actggacgcc
     6481 gacgcgtcag tgctgatttc aacaggtatc gacgccacgc aaaccaacca cggtcgccaa
     6541 catctcgacg aaacccaggt gcgggtgttt ggtcagcatc tgatgcaggg gatctacacc
     6601 acccaggatg gacgcagtga tgtggccatc agctgttgct gtatggtgag cggtgatgtg
     6661 cagcaatgct ataccgccaa agagcgccgt ttgcagcaac ataccagtgc gcagcttcat
     6721 gcaggcgaga cagtgacgtt gcaaaaactg gtgtggatcg actggcggga tgacaggcaa
     6781 gccgttttag acgagtgggg cagcgcgtcg cttcgccagc ttgaaatgtg cgcgcagcag
     6841 agttacgacc aacttcttgc agtatcaaca gaaaactggc gtcaatggtg gcagaaacgt
     6901 cgtatcacgg taaatggcgg cgaagcgcac gatcagcaag cgttagatta tgcgctttat
     6961 catctgcgca tcatgacgcc tgcccacgac gagcgcagca gcattgcggc aaaaggctta
     7021 accggcgaag gctacaaagg ccacgttttc tgggatacag aagtattttt gttaccgttt
     7081 catctgttta gcgatccgac ggttgcccga agtttactgc gttatcgctg gcacaacttg
     7141 ccaggcgcgc aggagaaagc gcgacgcaac ggctggcagg gcgcgctatt tccgtgggaa
     7201 agcgcgcgca gcggcgaaga agagacgccg gaatttgccg ccattaacat tcgcaccggg
     7261 ctgcggcaaa aagtggcctc ggcgcaggcg gaacatcatc tggtggccga tatcgcctgg
     7321 gcggttattc aatactggca gaccacgggg gatgaaagtt tcattgcgca tgaaggcatg
     7381 gcgctacttc tggaaactgc aaagttctgg attagccgcg cggtgagggt taacgaccgt
     7441 ctggaaattc atgatgttat tgggcctgac gaatataccg aacatgtcaa taataacgcc
     7501 ttcaccagct atatggcgta ttacaacgtc cagcaggcgc tgagtattgc ccgccagttt
     7561 ggctgtagcg acgatgcgtt tatccatcgc gccgaaatgt tccttaaaga actgcggctg
     7621 ccagaaattc agcccgacgg cgttttgccg caggatgatt cgtttatggc taagccggcg
     7681 attaatctgg cgaaatacaa agcggcggcg gggaagcaaa ccattctgct ggattattca
     7741 cgcgcagaag tgaacgagat gcaaatcctc aaacaagctg atgtggtgat gctcaattac
     7801 atgctgccgg agcagttctc agcggcatcg tgtcttgcca atctgcaatt ttatgaaccg
     7861 cgcactattc acgactcgtc attaagtaaa gcaatccacg gcattgttgc cgcacgctgt
     7921 ggcctgctga cccaaagtta tcagttctgg cgcgagggga ctgaaatcga tcttggtgct
     7981 gatccgcata gttgtgatga tggtatccat gctgccgcaa ctggcgctat ctggctgggg
     8041 gcgattcagg gttttgccgg ggtgagcgtg cgtgacggtg aattgcatct caatccggcg
     8101 ttacctgagc agtggcaaca gttgtctttc cctctgttct ggcagggctg cgaattacag
     8161 gtcactcttg acgcgcagcg tattgcgatt cgaacttctg cgcccgtttc actgcgtttg
     8221 aacggtcagc ttatatccgt ggctgaagaa tctgttttct gtttgggtga ttttattttg
     8281 cccttcaatg ggaccgctac cacacatcag gaggatgaat gaaactgcaa ggggtaattt
     8341 tcgatctgga tggtgtaatc accgataccg cgcatctgca tttccaggcg tggcagcaga
     8401 ttgccgctga aattggcatc agcattgatg cgcagtttaa cgaatcccta aaagggatca
     8461 gccgcgatga gtctctgcgg cgcattctgc aacacggggg caaagagggc gactttaact
     8521 cgcaggagag ggcgcaactg gcgtatcgca aaaatctgct ctatgtccac tcactgcgcg
     8581 agttgacggt cagcgctgtt ctacccggca ttcgctcttt gctggcagat ctccgtgcac
     8641 agcagatccc ggttgggctg gcttctgtct ccctgaatgc gccgacgatt ttagcggcgc
     8701 tggagctgcg cgagtttttc accttctgcg cggatgcttc ccaacttaaa aactcgaaac
     8761 cggacccgga aatctttctc gccgcctgtg ccgggctggg cgtgccgccg caggcatgta
     8821 tcggcattga agatgcgcag gcgggcattg acgccattaa cgccagcggt atgcgctcgg
     8881 tggggatcgg cgcgggctta accggggcgc aattactgtt gccttcaacg gactctctga
     8941 cctggtcgcg gttatcggcc ttctggcaaa acgtatagca aaggaatcaa catggctcag
     9001 ctttcgttac aacatattca aaaaatctac gataaccagg tgcatgtggt gaaggacttc
     9061 aacctggaaa ttgccgataa agagttcatc gtgtttgtcg gcccgtcggg ctgcggtaag
     9121 tcgaccaccc tgcgcatgat tgccgggctt gaggagatca gcggcggcga tctgttgatc
     9181 gacggcaaac gaatgaatga cgttccagcc aaagcacgca atatagcgat ggtgttccag
     9241 aactacgcgt tgtatccgca tatgacggtt tacgacaaca tggcgtttgg cctgaagatg
     9301 caaaaaatcg ccaaagaggt gattgatgag cgggtgaact gggcggcgca aattctcggc
     9361 ctgcgtgagt acctgaaacg caagccgggc gcgctttccg gcgggcaacg ccagcgtgtg
     9421 gcgctcgggc gggcgattgt gcgcgaagcg ggcgtgtttt taatggatga accgctctct
     9481 aaccttgatg ccaagctgcg cgtgcaaatg cgcgcagaga tcagcaagct acaccagaag
     9541 ctgaatacca ccatgatcta cgtgacccac gatcagaccg aagcgatgac catggcgacg
     9601 cggattgtga ttatgaagga tgggattgtt cagcaagttg gtgcgccgaa aacggtttat
     9661 aaccaaccag cgaatatgtt tgttgccgga tttattggat caccggcgat gaattttatt
     9721 cgtggcacga tcgatggcga taaattcgtt acggaaacgc ttaaattaac cattcccgaa
     9781 gagaaattag cggttctgaa aacacaggga agtttgcata agcccatcgt gatgggaata
     9841 cgtccggaag atattcatcc ggacgcgcaa gaggaaaata acatttccgc caaaattagc
     9901 gtggcagaat taaccggtgc ggaatttatg ctctacacca cggttggggg gcacgagtta
     9961 gtggtccgtg ctggtgcgtt aaatgattat catgcaggag aaaatatcac tattcatttt
    10021 gatatgacga aatgtcattt ctttgatgca gaaacggaaa tagcaattcg ctaaatacag
    10081 ggggaaggca ttcccccaga ataatacaag gaacaataat gaaaaagtta ttaccctgta
    10141 ccgcactggt gatgtgtgcg ggaatggcct gcgcacaggc cgaggaaagg aacgactggc
    10201 actttaatat cggcgcgatg tacgaaatag aaaacgtcga gggttatggc gaagatatgg
    10261 atgggctggc ggagccttca gtctatttta atgccgccaa cgggccgtgg agaattgctc
    10321 tggcctatta tcaggaaggg ccggtagatt atagcgcggg taaacgtgga acgtggtttg
    10381 atcgcccgga gctggaggtg cattatcagt tcctcgaaaa cgatgatttc agtttcggcc
    10441 tgaccggcgg tttccgtaat tatggttatc actacgttga tgaaccgggt aaagacacgg
    10501 cgaatatgca gcgctggaaa atcgcgccag actgggatgt gaaactgact gacgatttac
    10561 gtttcaacgg ttggttgtcg atgtataaat ttgccaacga tctgaacacg accggttacg
    10621 ctgatacccg agtcgaaacg gaaacaggcc tgcaatatac cttcaacgaa acggttgcct
    10681 tgcgagtgaa ctattatctc gagcgcggct ttaatatgga cgacagccgc aataacggtg
    10741 agttttctac gcaagaaatt cgcgcctatt tgccgctgac gctcggcaac cactcggtga
    10801 cgccgtatac gcgcattggg ctggatcgct ggagtaactg ggactggcag gatgatattg
    10861 aacgtgaagg ccatgatttt aaccgtgtag gtttatttta cggttatgat ttccagaacg
    10921 ggctttccgt ttcgctggaa tacgcgtttg agtggcagga tcacgacgaa ggcgacagcg
    10981 ataaattcca ttatgcaggt gtcggcgtaa attactcgtt ctgataatgg gctaaattgc
    11041 cggatgcggc acgagtactt tatccgaact ataaatgtag gccggataag atgcgctagc
    11101 atcgcatccg gcattcaggc aaggtagctg gtatttattt cagcgtcata tgcgtggcaa
    11161 cggtaatatt ctgtggtgac ggttttccag aaattaagcg gaataataac tcacagcttt
    11221 gttgacctaa ctcctgtgtt gggatatcaa taccgcctgg cgcaggcgtt aaaataaacg
    11281 acagcgtttc attactataa cccaccaccg ctaactgctg cggaatagca atatttttct
    11341 ctgctgccgc acgataaata ctcattaatt tcaggctgtc agtagcaaac actgcctcag
    11401 gcaacggtga ctggcttaat aattcccgcg cggcttgcag tgctgtttca tgggtataac
    11461 cgccgtcaac aatccattca tcacgcactg caatattatg cgcagccagg ctctgcttat
    11521 aaccatttac ccgatccact gaaacatgga catcaagcgg agcatgcagg caggcaatat
    11581 tttgatgccc actttcaatt aatgcatcgg tcaacgcaat gctgtcgcca aaattatcgg
    11641 tatcgacaga ataaacatgg gcatattgac cttcaacttt gccaatcacc acgacaggaa
    11701 tatcgtattt atcaagttgg gcaaaaaatg actcatccgc tggcgaactt agcataataa
    11761 tgcctttaat cattttctgc ttaattttgc tttcacattt ttgtaagtct tctgccggat
    11821 tgtgcgacgt ctgcaatatc acgtcgaaac cttcctcttc agctttggcg gtgatggcat
    11881 gcaaaacttc agagaaaaac ggattacccg ccgtagtttt ggtcgaacgg gtagaaatca
    11941 ccataatggc atcaaaaccc gaagaggtca gcgcgcgggc cagcttgttt ggctgatact
    12001 gtaattcttc aatggcccgt aacacttttt cgcgcgcttc cggggagata ttagtttgct
    12061 tattcagcac gcgtgatacg gtggattttg atacgcctgc aaccctggca atatcataaa
    12121 tagtagggga cataggtcag gaactccgtc cgaacgtgaa tgctgcacat cttatggagt
    12181 tcgcccgtcg atgacaacac gggcagttga taatcaatgg cctggccccc acattcatat
    12241 ccttacgaat gatttttttc tggtcttcag agcagcacag gacagcaatg aagcgactta
    12301 aaaatgaact taatgcgctg gtgaatcggg gtgtcgacag acatctgcgc ctcgccgtaa
    12361 ccggactaag ccgcagcggc aaaacagcgt ttatcactgc gatggtcaat cagttgctca
    12421 atattcacgc cggagcacgc ttgccgctgt taagcgcagt gcgtgaagag cgcctgctgg
    12481 gcgtaaaacg cattcctcag cgtgactttg gcattccgcg cttcacatat gatgaaggac
    12541 tggcgcagtt atatggcgat cctcccgcct ggccgacgcc aacgcgcggc gtcagtgaaa
    12601 tccgcctggc gctacgtttt aaatcgaacg attcgctgct acgccacttc aaggacacct
    12661 ccacgctgta tctggagatt gtggattatc ccggcgaatg gttgctcgac ctgccgatgc
    12721 tggcgcagga ctatttaagc tggtcacgcc agatgacggg cttactcaat ggtcagcgcg
    12781 gcgaatggtc ggtcaaatgg cgaatgatgt gcgaagggct ggacccgcta gcgcctgccg
    12841 acgaaaaccg gctggcagac attgccgccg cgtggaccga ttatctccac cactgtaaac
    12901 agcaggggct gcactttatt cagcctgggc gctttgtctt gccgggagat atggcaggtg
    12961 cgcccgcgct gcaattcttc ccgtggccgg atgtcgatgc ctggggcgag tccaaactgg
    13021 cgcaggccga taagcacacc aatgccggaa tgctgcgcga gcggtttaat tattactgcg
    13081 agaaggtggt gaaggggttc tataagaatc attttctgcg ctttgaccgc cagattgtgc
    13141 tggttgattg cctgcaacct ctcaacagtg ggccacaggc atttaatgat atgcgtctgg
    13201 cactgacgca gctgatgcaa agtttccact acgggcagcg taccttgttc cggcgtttgt
    13261 tttcgccggt tatcgataag ctattgtttg ctgccactaa agcggaccat gtgaccatcg
    13321 atcagcacgc caatatggtt tcattactgc aacaactgat tcaggatgcc tggcaaaatg
    13381 cggcgtttga agggattagt atggattgcc tggggctggc ctcggtgcag gcgaccacca
    13441 gcggcattat tgacgtcaac ggcgagaaaa ttccggcatt gcgcggtaac cggctcagcg
    13501 atggtgcgcc actcaccgtt tatcccggtg aagttcctgc gcgtttgccc ggtcaggcgt
    13561 tctgggacaa gcaagggttc cagtttgaag cgtttcgccc gcaggtgatg gatgtcgaca
    13621 aaccgctgcc gcatattcgt cttgatgccg cgcttgaatt tttaatagga gataaattgc
    13681 gatgaccgaa ccgttaaaac cacgtattga tttcgacggt ccgctggagg tcgatcagaa
    13741 tccaaaattc agggcgcagc agacctttga cgaaaatcag gcgcaaaatt ttgccccggc
    13801 cacgctcgat gaagcgcctg aagaagaggg gcaagttgaa gcggtaatgg atgcagcgtt
    13861 acgtccgaaa cgcagcctgt ggcgcaaaat ggtgatgggc gggctggctc tgtttggcgc
    13921 aagcgttgtc gggcagggtg tacagtggac aatgaatgcc tggcaaactc aggactgggt
    13981 ggcgctgggt ggatgtgccg ctggggcatt gattatcggc gctggcgtag gttctgtggt
    14041 aacagagtgg cggcgcttat ggcgcttgcg acagcgcgcc catgaacgcg acgaagcgcg
    14101 cgatttgttg cacagccacg gcacgggcaa aggccgcgca ttttgcgaaa aactggcgca
    14161 gcaggcgggt attgatcagt ctcatccagc gctgcaacgc tggtatgcct caatccatga
    14221 aacgcaaaac gaccgtgaag tggtcagttt gtatgcgcat ttggtccagc cagttttaga
    14281 tgcccaggcg cggcgcgaaa tcagccgttc ggcggcggaa tcaacgttga tgattgcggt
    14341 cagcccgctg gcgttggtcg atatggcgtt tatcgcctgg cgcaatctgc gtttaattaa
    14401 tcgcatcgcc acgctgtatg gcattgaact ggggtattac agccgtttgc gtctgtttaa
    14461 gctggtattg ctgaatatcg cttttgccgg agccagcgaa ctggtgcgcg aagtggggat
    14521 ggactggatg tcgcaagatc tcgctgctcg tttgtctacc cgcgcagctc agggaattgg
    14581 tgctggactt ctgacggcac gactcgggat taaagctatg gagctttgcc gcccgctgcc
    14641 gtggattgac gatgacaaac ctcgcctcgg ggatttccgt cgtcagctta tcggtcaggt
    14701 gaaagaaacg ctgcaaaaag gcaaaacgcc cagcgaaaaa taatgcaata tcgggtgctg
    14761 accggatatc tttacgccga agtgcccgtt tttccgtctt tgtgtcaatg attgttgaca
    14821 gaaaccttcc tgctatccaa atagtgtcat atcatcatat taattgttct tttttcaggt
    14881 gaaggttccc atgcgtctgg aagtcttttg tgaagaccga ctcggtctga cccgcgaatt
    14941 actcgatcta ctcgtgctaa gaggcattga tttacgcggt attgagattg atcccattgg
    15001 gcgaatctac ctcaattttg ctgaactgga gtttgagagt ttcagcagtc tgatggccga
    15061 aatacgccgt attgcaggtg ttaccgatgt gcgtactgtc ccgtggatgc cttccgaacg
    15121 tgagcatctg gcgttgagcg cgttactgga ggcgttgccc gaacctgtgc tctctgtcga
    15181 tatgaaaagc aaagtggata tggcgaaccc ggcgagctgt cagctttttg cgcaaaaatt
    15241 ggatcgcctg cgcaaccata ccgccgcaca attgattaac ggctttaatt ttttacgttg
    15301 gctggaaagc gaaccgcaag attcccataa cgagcatgtc gttattaatg ggcagaattt
    15361 cctgatggag attacgcctg tttatcttca ggatgaaaat gatcaacacg tcctgaccgg
    15421 tgcggtggtg atgttgcgat caacgattcg tatgggccgc cagttgcaaa atgtcgccgc
    15481 ccaggacgtc agcgccttca gtcaaattgt cgccgtcagc ccgaaaatga agcatgttgt
    15541 cgaacaggcg cagaaactgg cgatgctaag cgcgccgctg ctgattacgg gtgacacagg
    15601 tacaggtaaa gatctctttg cctacgcctg ccatcaggca agccccagag cgggcaaacc
    15661 ttacctggcg ctgaactgtg cgtctatacc ggaagatgcg gtcgagagtg aactgtttgg
    15721 tcatgctccg gaagggaaga aaggattctt tgagcaggcg aacggtggtt cggtgctgtt
    15781 ggatgaaata ggggaaatgt caccacggat gcaggcgaaa ttactgcgtt tccttaatga
    15841 tggcactttc cgtcgggttg gcgaagacca tgaggtgcat gtcgatgtgc gggtgatttg
    15901 cgctacgcag aagaatctgg tcgaactggt gcaaaaaggc atgttccgtg aagatctcta
    15961 ttatcgtctg aacgtgttga cgctcaatct gccgccgcta cgtgactgtc cgcaggacat
    16021 catgccgtta actgagctgt tcgtcgcccg ctttgccgac gagcagggcg tgccgcgtcc
    16081 gaaactggcc gctgacctga atactgtact tacgcgttat gcgtggccgg gaaatgtgcg
    16141 gcagttaaag aacgctatct atcgcgcact gacacaactg gacggttatg agctgcgtcc
    16201 acaggatatt ttgttgccgg attatgacgc cgcaacggta gccgtgggcg aagatgcgat
    16261 ggaaggttcg ctggacgaaa tcaccagccg ttttgaacgc tcggtattaa cccagcttta
    16321 tcgcaattat cccagcacgc gcaaactggc aaaacgtctc ggcgtttcac ataccgcgat
    16381 tgccaataag ttgcgggaat atggtctgag tcagaagaag aacgaagagt aagcgcgaat
    16441 atgcctgatg gtgcaacacc atcaggcata ttaacttatg ctttcagtac agccagagct
    16501 gcttcgtaat ccggctcggt ggtgatttca tccaccagct ggctgaaaat cacattgtca
    16561 ttttcgtcaa taaccacaac ggcacgcgct gccagacctt tcagtgggcc atcagcaatt
    16621 gccacaccga aggcttgcag aaattcagcg ttacggaaag tggagagggt gataacgttg
    16681 ttcagacctt ctgcgccgca gaaacgagac tgggcgaacg gcagatcggc agagatacac
    16741 agcacaacgg tgttgtcgat ctcagttgcc agttggttaa acttacgtac tgatgcggcg
    16801 caaacaccgg tatcaatact cgggaaaatg ttcagcactt tgcgtttacc cgcaaactga
    16861 ccgagggtga cgtcagacag atcttttgcc acgagagtaa aagtctgcgc tttgctaccc
    16921 gcctgcggga tggaattggc gactgtaacc gggttgccct ggaaatgaac ggtttgtgac
    16981 atgattatct ttcctgttta catatagtta acgtcacacc tagtttatgc taactgtcaa
    17041 taacacagca aacgctattt gcgcttaatc cgcagaccac cgcgacaaca aggagtaaag
    17101 atgagaaccg ttaaggtatt tgaggaagcc tggcccttac ataccccgtt tgtgattgcc
    17161 cggggaagtc gcagtgaagc gcgcgtggtg gtggttgaac tggaagaaga gggtattaaa
    17221 ggcaccggcg aatgcacgcc gtatccgcgt tatggggaaa gtgatgcctc ggtaatggcg
    17281 caaattatga gcgtcgtgcc gcaattagag aaagggctga cacgggagga gttgcaaaaa
    17341 attctccctg ccggcgcagc acgtaatgcg ctggattgtg cattgtggga tctggccgcg
    17401 cgaaaacagc agcaatcgct ggctgatttg atcggcataa cgcttcccgg gacagtcacc
    17461 actgcacaga cggtagtcat cggtacgcct gatcagatgg ccaatagtgc atcaacactc
    17521 tggcaggcag gcgcgaaatt actgaaagtg aagctggata accatcttat cagtgagcgg
    17581 atggtggcaa ttcgcacagc tgtgcccgat gcgacgctga tcgttgacgc aaatgaatcc
    17641 tggcgtgcag aagggttggc ggcgcgttgc cagctattgg cggatttagg cgttgcgatg
    17701 cttgaacaac cgcttcctgc gcaggacgat gcggcgctgg agaattttat tcatccgtta
    17761 ccgatttgtg cagatgaaag ttgtcataca cgtagcaatc tgaaggcgct gaaagggcgc
    17821 tatgagatgg ttaacattaa gcttgataaa accggtggtc tgacggaagc gctggcgctg
    17881 gcgactgaag cgcgtgcaca aggttttcgt ctgatgctgg gctgcatgtt gtgtacctct
    17941 cgggccatta gcgccgcttt accgctggtg ccgcaggtca gtttcgtcga tcttgacgga
    18001 ccgacctggc tggcggtaga tgtggaaccg gcgcttcagt tcacgacggg cgaattgcat
    18061 ctttaggatg ccagcgcagc aagtttgcca tcgcaaagag atatttttcg ctggcttcgt
    18121 cggaggagat gggcggaaac tctgcggtga tgcaatgcag gttgagatcc gcacaccaac
    18181 taccgaaaga gccaggtgtt tcataaccga cgctggttac cagaggcaat tcaaacgcct
    18241 gggccagcca ttcgcctaat tcgctgtgtc tgggatcttc aatacaggcc agcggatcgt
    18301 ggaatgagac cacccaggcg ggttgaatgc ggtgtataag ctggcacagc gcctgggttt
    18361 caggttcaga gccaggtttg tcacctgtca gcaatacgac atcacgttct tcagcggcgc
    18421 tgttccagcg ataaactgtt tcaccttcct tccagttcgc cgccggaaag tttcggttta
    18481 aatccacacc attagcattg gcccgtaacc ccagctggca gccgtcagga ttcacacaca
    18541 gcactacatg atggcggcgc aaagaaggtg tcaaagtccg cagagcacag gagagcgtga
    18601 cgaccgaaga gttttcatca ccgtgagtgc cagccagaat caaaccactt tcgcgactgg
    18661 ctgcagtggc cggaaaccag attaacggcg cgcccaataa tgaacgtccg taatgttctg
    18721 ttccgggcgg aaatgcgccg cgttcggcgc gtgggcggtt tacggtcatg atcgtctctg
    18781 gttcaggaag tattacaggc agtgttgtgc aaattttgcg gattatcaaa tccttttctt
    18841 caacgctatg tcatcgatct tgtatcactt ttttgccgga agttgtttgc atttttattg
    18901 atgtgcagat attagaatta tgctgactta gatgtaagtc gtaatgttat atacatggcg
    18961 tattttattc ttactggttc tacacatgtt aatcgcatcg gattgatcag ttcaccttaa
    19021 taagtttaat aaatcgtact gcacatagta cattacgatg gtaactatgt aattgattta
    19081 catgaataat ggcttgtcac catcagtcac gggttgattg atacctatat tctcaccaat
    19141 gtgtttctca ggtttatcga tgatatctgt gattaatgct gcgacactct tacgtgatac
    19201 gatcgtacct ttgaatggtt cattgcgtga ggtaagctca taatcaataa tatcttcatc
    19261 agtcagccag gcagggcgaa gaattgtata ttccagtcca gaagcctcaa ttgcatccgc
    19321 agccctgcgg aatggtttta ggggctcacc aataactgca ttattccatt cgccaaattt
    19381 tcctggaact tcatcataaa taccgaggga aagaacaaag atcagacgtt taacatcaca
    19441 ggctttcatc gcagcgatca ctgaatttgc ctgaatatct aaatcttccc ccgtaaggtt
    19501 ggcatacaca acgtcctgtc cctgcatggc ctgcttcagt gctgcatgat tcagtacatc
    19561 tcccataata atcttgctat tagttggata gggtttgtgg atttttgctg gctgacgggc
    19621 aaataaagtt tgtttaatgg tttgtttatc tgccagttga ttaatcacat ggcgggctat
    19681 ctgaccgcca gcaccaagaa tcagtacatt tttcataata tttctcatga aacttatccc
    19741 agatgggtgt tgaaaaaagg aataactttt tccagcgcct tctgaactgc ttccggtttg
    19801 tcatacagtt cataatgtga gaaatcgggt aatgaaacta actgacgatc ctgcgaaacc
    19861 atcgcccggc catagacttc cagtccatca cgataagcac cgaaggcacc gactttttca
    19921 ccaactacta ccatcaccgg ctgagtcagt aacacttcag taaatgcgaa ggcatcccag
    19981 gccagcgttt tctgtgcatg tgagaacaac atttttgtcg cacctcccgg ttgttgcccg
    20041 cgtggtgttt tatagtagtc agttgcttca taaacatcgc gttcggtcag tccatgggct
    20101 tttgctgcat ccaggctggc gggaagtaat tcgttgatct gtaattctcc accacgagcc
    20161 tctttggttc gttgcgacgc cattgcattc aatgcgccaa tcggatcgta attgctgaaa
    20221 ccttcccgaa aaagacgccc gatattaaca cccgtaatac tcactactgc tttaattcgt
    20281 ttttctgtca gcgcggcatt aatggcatag ccaccacccc cacacactcc cagcacgcca
    20341 atacgttcag cattaacata tggcagagtt actgcataat cgataacacg gctaatatcc
    20401 tctacgcgct gtgtcggatc ttctatccag cgaggctccc caccagattc tccctggaaa
    20461 ctggcgtcat aagcgagaac gagatagcct ttttcagcca atgctttgcc atagatattg
    20521 ccagacgtct gctccttaca actgccaaaa ggatggacgc tgataatcgc cggatactgt
    20581 tttgactcat caaaatttgg tggaaactga atattggcag caatatgcca ggccatatca
    20641 ctattgcgga tttgaatatg tttcattaca gagtttcctc aaagaacgga gctaatactg
    20701 aaattgcttc agccacatag gctttgccgt cataaagatc catatggttt gcgccttcaa
    20761 caatgtgata gcgtttatcc tggcttgatg ctcgatcgta caggtcgtca ctcatccatt
    20821 tgctccctgc ctggctgccc gccacaatct gcgtcggctg agtcaggtac acttccgcca
    20881 tatggtaagc atcataggta ataatctggt taaggctgcg caaagtagcg taacccggtg
    20941 ctgttggata ctgcgcgcga ggggtgtggt aatactccca ggcctgacgc agttcttcgt
    21001 tcggcgcatc ggactccttc attggtgcca gtggcataat ggcgtattct ccgctgctaa
    21061 tatcgctggt tctggcgttt gaacccgctt caacgtatgg cagggcatca atagatttca
    21121 cattgttttc ccaaccatta cggaatatcg aaccaatatt gaccgcacta acggtaccga
    21181 tggccttgat gcggcgatcc tgaattgcag cattggctgt atatcctgca ccggcacaaa
    21241 ttcccatcgc accaattcgg gtattgtcga cataagaaag cgttgtcagg taatcaatca
    21301 cggcactgat gtcttcagta cgaatgtatg ggttttctaa ctgacgcggc tcgccgccac
    21361 tttcaccttg ataagatgcg tcataagcaa tagtgacaaa tcccttttcc gccagttttt
    21421 tggcataggt tccggccgtt tgttctttaa cgcccccacc tgggtgagat accacaattg
    21481 cctgatactg acgggtttca tcaaattttg gagggaaata gctcactgca gacaaagaga
    21541 tggttggatt attgctgtta gtgaagctga ctttattatt catcattccg ttcctcatgg
    21601 agttgtcggt tcgttttaac ggttggtgat atcactatag atattgatca ttaagttgat
    21661 tagacccaaa atcatgatta gacctatcat ttaaatgatt aatagattgc atggagatac
    21721 tgaatgaagc gtgaagaaat cgctgatctg atggcgtttg tcgtcgttgc agaggagcgt
    21781 agcttcactc gtgcagcagc ccgcctgagc atggcgcagt cagctttaag ccagatagtg
    21841 cgtcgtatag aagaacgatt gggattgcgg cttctgacgc gaaccacgcg cagcgttgtt
    21901 ccaactgaag cgggcgagca tcttttgtct gttcttggcc ctatgttgca tgacatagat
    21961 tcagccctgg catccctgag cgagctgcag aaccgcccat ccgggacaat acgtattact
    22021 actgtagaac atgcagcaaa aacgatattg ttaccagcaa tgcgcacatt cctgaaatcg
    22081 catcctgaaa ttgatattca gctcaccatt gattatggtt tgaccgatgt cgtttctgaa
    22141 cgttttgatg caggcgtccg tctgggtggg gagatggata aagatatgat cgccattcga
    22201 atcgggccag atataccaat ggctattgtt ggctcaccgg attatttttc tcgccgaagt
    22261 gttccaacgt cagtgtcaca attaatagat catcaggcaa ttaatttgta tcttcccaca
    22321 tcgggtacag caaatcgctg gagattaata cgcggtggac gtgaagttcg tgttcgcatg
    22381 gaaggtcagc ttttactgaa tacgatagac ctgatcattg atgctgcaat tgatgggcat
    22441 ggattggcgt atctacctta tgatcaggtt gagcgggcta ttaaagaaaa aaaactgata
    22501 cgtgttttgg ataaattcac accagattta cccggtcatc acctgtacta tccacaccgt
    22561 cgacatgctg gctcggcatt ctcattattt atagataggc tgaagtataa aggtgctgtt
    22621 tagcactact tgctgataca ttaatttaat ttctctctta acgtattctc agttcctttc
    22681 aacgttttgg ttatttttta ttcttcgtac aatggcgaca gatgctgatt atgataccga
    22741 aaactggttt gaacgtgcga agcctgaacg tagtgttcgg tgttctatgt gctttaccgc
    22801 attttggcgt cgattattta cactaaatct gatttaatat attgatacat aaaacatttt
    22861 atgcttcctg tgtcactttt ttgatggaag ttgtttgcat ttctttaagg cgaaacaaat
    22921 aattacgcat caattttaat gtcggttaga gggaaactta tgaagcactc tgtttcagtc
    22981 acgtgttgtg cgctgttggt cagcagcatt tctctttcgt atgctgcaga agttccgagc
    23041 ggcacagtac tggcagagaa gcaggagctg gtgcgccaca ttaaagatga gcctgcgtcg
    23101 ctggatcccg ctaaagccgt gggcctgcca gagattcagg tcattcgcga tctgtttgaa
    23161 ggactggtga atcagaacga gaaaggggag attgtccccg gcgttgcgac tcagtggaaa
    23221 agtaatgaca accgtatctg gacttttacc ctgcgcgata acgcaaaatg ggcggatggc
    23281 acaccggtaa cggcgcaaga ttttgtctac agctggcaac gtctggtgga cccgaaaacg
    23341 ctatcgccat ttgcatggtt tgccgcgctg gcgggaatca acaacgcaca ggcgattatt
    23401 gatggtaaag ctacgccaga ccagcttggc gtcaccgcag ttgatgccca tactttgaaa
    23461 attcagcttg ataaaccgtt gccgtggttt gtgaatttaa ccgctaactt tgccttcttc
    23521 ccggtgcaaa aagccaacgt agaaagcggt aaagagtgga cgaaacccgg aaatctgatc
    23581 ggcaatggcg cttatgttct taaagatcgc gtagtcaatg aaaaactggt cgtggtaccg
    23641 aatacccatt attgggataa cgccaaaacg gtactgcaaa aagtgacctt cctgccaatt
    23701 aatcaggaat ccgcagccac taagcgttac ctcgcggggg atattgatat caccgaatcc
    23761 ttcccgaaaa atatgtatca gaagctgttg aaggatattc cggggcaggt ttatacgccg
    23821 ccacagctcg ggacctatta ttatgcgttt aacacgcaaa aagggccgac ggcagatcag
    23881 cgcgttcgcc tggcattaag tatgacgata gatcgccgcc tgatgaccga aaaagtgttg
    23941 ggaacgggcg aaaagccagc gtggcatttt acaccagatg ttaccgccgg atttacgccg
    24001 gaaccttcgc cgtttgaaca aatgagtcag gaagaactga atgcgcaggc aaaaactttg
    24061 ttgagcgcag ctggttatgg tccgcaaaaa ccgctgaagc tgacgctttt gtataacact
    24121 tcagaaaacc atcaaaaaat tgcgattgct gtagcatcga tgtggaaaaa gaaccttggc
    24181 gtagatgtta aattgcaaaa tcaggaatgg aaaacctata tcgatagccg taacaccggc
    24241 aattttgatg ttatccgcgc ctcatgggtg ggggattata atgaaccctc cactttcctg
    24301 acattattaa cgtcaacgca ttcaggaaat atttcacgct ttaacaatcc ggcatacgac
    24361 aaagttctgg cccaggcatc gatggaaaat accgttaaag cgcgtaatgc cgattacaac
    24421 gcggcagaaa aaatcctcat ggagcaagca ccgattgcac caatttatca atataccaat
    24481 ggacgattaa tcaagccgtg gctgaaaggt tatcccatta ataacccgga agatgttgcg
    24541 tacagccgga ctatgtatat tgtgaagcat tgatgtgatg ggaactggcg ttacccttgt
    24601 ggataacgcc agtgatgtct gatttagcgc ccctgttccg gcggtgtaat gttatccata
    24661 tacagcgtct ggctggggaa ggcaaagtcc gcgccgtgtg actgcacaat atcgataatc
    24721 ttcaaataaa cgtcttgctg tgcagcaagc cattcagccc ataccgtggt tttggtaaag
    24781 caataaacca taatattcaa tgacgagtcg gcaaactggt tgaaataaac cagtaaggtt
    24841 tgtcgctggt cgatggccgg gtgatttttc agcatctcac gtacggcttc gacaataacg
    24901 cccacttttg ccgcatcctc ataacgtaaa ccaatggtcg tggtaatgcg gcggttggtc
    24961 attcgtcctg ggttttctac gctgatcgac gaaaacagcg agttcggtac gtacaatgga
    25021 cgattatcaa aggtcgtaat tttggtaatt cgccagccaa tttccgctac tgtaccttcg
    25081 atatttctgt ccggtgaacg gatccagtcg ccaatactga aaggacggtc gaaatagagc
    25141 atgatcccgg aaaagaaatt actcagaata tctttaccgg ccataccgac agccagacca
    25201 ccaataccac caaaggtcag caagccagaa aggctcatgc cgaaatgttc gccataaagc
    25261 agaacaagca ccacaataat ggtgattttg ataatgcgcg acataatccg cgcactggtg
    25321 atatcgcgac cttttttaat ctgctgtttt tcaaactgat taatcagcaa aaatagctta
    25381 atcgtcagaa taaccgcaat cagggacgta cagataaaat cgataacgcc tggggtgata
    25441 aatttg
//
LOCUS       DAZKHF020000041        25207 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2201S31241
            SAMN43267800-rid25106413.denovo.041, whole genome shotgun sequence.
ACCESSION   DAZKHF020000041 DAZKHF020000000
VERSION     DAZKHF020000041.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267800
            Sequence Read Archive: SRR30307263
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 25207)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25207)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 25207)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350581.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350581.2
            Genome Coverage       :: 151x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:21:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,652
            CDSs (total)                      :: 5,557
            Genes (coding)                    :: 5,320
            CDSs (with protein)               :: 5,320
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 237
            CDSs (without protein)            :: 237
            Pseudo Genes (ambiguous residues) :: 0 of 237
            Pseudo Genes (frameshifted)       :: 102 of 237
            Pseudo Genes (incomplete)         :: 130 of 237
            Pseudo Genes (internal stop)      :: 52 of 237
            Pseudo Genes (multiple problems)  :: 41 of 237
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25207
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267800-rid25106413.denovo.041"
                     /serotype="O 26:H11"
                     /isolate="2201S31241"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-01"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            102..1043
                     /locus_tag="ACHOSC_RS06920"
                     /old_locus_tag="pgaptmp_001384"
     CDS             102..1043
                     /locus_tag="ACHOSC_RS06920"
                     /old_locus_tag="pgaptmp_001384"
                     /EC_number="2.7.1.83"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine kinase"
                     /protein_id="HGU4547358.1"
                     /translation="MREKDYVVIIGSANIDVAGYSHESLNYADSNPGKIKFTPGGVGR
                     NIAQNLALLGNKAWLLSAVGSDFYGQSLLTQTNQSGVYVDKCLIVPGENTSSYLSLLD
                     NTGEMLVAINDMNISNAITAEYLAQHREFIQRAKVIVADCNISEEALAWILDNAANVP
                     VFVDPVSAWKCVKVRDRLNQIHTLKPNRLEAETLSGIALSGRGDVAKVAAWFHQHGLN
                     RLVLSMGGDGVYYSDISGESGWSAPIKTNVINVTGAGDAMMAGLASCWVDGMPFAESV
                     RFAQGCSSMALSCEYTNNPDLSIANVISLVENAECLN"
     gene            1031..1969
                     /gene="psuG"
                     /locus_tag="ACHOSC_RS06925"
                     /old_locus_tag="pgaptmp_001385"
     CDS             1031..1969
                     /gene="psuG"
                     /locus_tag="ACHOSC_RS06925"
                     /old_locus_tag="pgaptmp_001385"
                     /EC_number="4.2.1.70"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine-5'-phosphate glycosidase"
                     /protein_id="HGU4547359.1"
                     /translation="MSELKISPELLQISPEVQDALKNKKPVVALESTIISHGMPFPQN
                     AQTAIEVEETIRKQGAVPATIAIIGGVMKVGLSKEEIELLGREGHNVTKVSRRDLPFV
                     VAAGKNGATTVASTMIIAALAGIKVFATGGIGGVHRGAEHTFDISADLQELANTNVTV
                     VCAGAKSILDLGLTTEYLETFGVPLIGYQTKALPAFFCRTSPFEVSIRLDSATEIARA
                     MAVKWQTGLNGGLVVANPIPEQFAMPEESINAAIDQAVAEAEEQGVIGKESTPFLLAR
                     VAELTGGDSLKSNIQLVFNNAILASEIAKEYQRLAG"
     gene            2063..3313
                     /locus_tag="ACHOSC_RS06930"
                     /old_locus_tag="pgaptmp_001386"
     CDS             2063..3313
                     /locus_tag="ACHOSC_RS06930"
                     /old_locus_tag="pgaptmp_001386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005337 - nucleoside transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0015293 - symporter activity [Evidence
                     IEA];
                     GO_process: GO:1901642 - nucleoside transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NupC/NupG family nucleoside CNT transporter"
                     /protein_id="HGU4547360.1"
                     /translation="MDIMRSVVGMVVLLAIAFLLSVNKKSISLRTVGAALLLQIAIGG
                     IMLYFPPGKWAVEQAALGVHKVMSYSDAGSAFIFGSLVGPKMDVLFDGAGFIFAFRVL
                     PAIIFVTALISLLYYIGVMGLLIRILGSIFQKALNISKIESFVAVTTIFLGQNEIPAI
                     VKPFIDRMNRNELFTAICSGMASIAGSMMIGYAGMGVPIDYLLAASLMAIPGGILFAR
                     ILSPATEPSQVTFENLSFSETPPKSFIEAAASGAMTGLKIAAGVATVVMAFVAIIALI
                     NGIIGGIGGWFGFANASLESIFGYVLAPLAWIMGVDWSDANLAGSLIGQKLAINEFVA
                     YLSFSPYLQTGGTLEVKTIAIISFALCGFANFGSIGVVVGAFSAISPKRAPEIAQLGL
                     RALAAATLSNLMSATIAGFFIGLA"
     gene            complement(3381..4079)
                     /locus_tag="ACHOSC_RS06935"
                     /old_locus_tag="pgaptmp_001387"
     CDS             complement(3381..4079)
                     /locus_tag="ACHOSC_RS06935"
                     /old_locus_tag="pgaptmp_001387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311082.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator YeiL"
                     /protein_id="HGU4547361.1"
                     /translation="MKEIHNNDLKQQLMSESAFKDCFLTDVSADTRLFHFLARDYIVQ
                     EGQQPSWLFYLTRGRARLYATLANGRVSLIDFFAAPCFIGEIELIDKDHEPRAVQAIE
                     ECWCLALPMKHYRPRLLNDTLFLRKLCVTLSHKNYRNIVSLTQNQSFPLVNRLAAFIL
                     LSQEGDLYHEKHTQAAEYLGVSYRHLLYVLAQFIHDGLLIKSKKGYLIKNRKQLSGLA
                     LEMDPENKFSGMMQ"
     gene            4209..5150
                     /gene="rihB"
                     /locus_tag="ACHOSC_RS06940"
                     /old_locus_tag="pgaptmp_001388"
     CDS             4209..5150
                     /gene="rihB"
                     /locus_tag="ACHOSC_RS06940"
                     /old_locus_tag="pgaptmp_001388"
                     /EC_number="3.2.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosylpyrimidine nucleosidase"
                     /protein_id="HGU4547362.1"
                     /translation="MEKRKIILDCDPGHDDAIAMMMAAKHPAIDLLGITIVAGNQTLD
                     KTLINGLNVCQKLEINVPVYAGMPQPIMRQQIVADNIHGETGLDGPVFEPLTRQAEST
                     HAVKYIIDTLMASDGDITLVPVGPLSNIAVAMRMQPAILPKIREIVLMGGAYGTGNFT
                     PSAEFNIFADPEAARVVFTSGVPLVMMGLDLTNQTVCTPDVIARMERAGGPAGELFSD
                     IMNFTLKTQFENYGLAGGPVHDATCIGYLINPDGIKTQEMYVEVDVNSGPCYGRTVCD
                     ELGVLGKPANTKVGITIDTDWFWGLVEECVRGYIKTH"
     gene            5250..6500
                     /locus_tag="ACHOSC_RS06945"
                     /old_locus_tag="pgaptmp_001389"
     CDS             5250..6500
                     /locus_tag="ACHOSC_RS06945"
                     /old_locus_tag="pgaptmp_001389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005337 - nucleoside transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0015293 - symporter activity [Evidence
                     IEA];
                     GO_process: GO:1901642 - nucleoside transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NupC/NupG family nucleoside CNT transporter"
                     /protein_id="HGU4547363.1"
                     /translation="MDVMRSVLGMVVLLAIAFLLSVNKKKISLRTVGAALVLQVVIGG
                     IMLWLPPGRWVAEKVAFGVHKVMAYSDAGSAFIFGSLVGPKMDTLFDGAGFIFGFRVL
                     PAIIFVTALVSILYYIGVMGILIRILGGIFQKALNISKIESFVAVTTIFLGQNEIPAI
                     VKPFIDRLNRNELFTAICSGMASIAGSTMIGYAALGVPVEYLLAASLMAIPGGILFAR
                     LLSPATESSQVSFNNLSFTETPPKSIIEAAATGAMTGLKIAAGVATVVMAFVAIIALI
                     NGIIGGVGGWFGFAHASLESILGYLLAPLAWVMGVDWSDANLAGSLIGQKLAINEFVA
                     YLNFSPYLQTGGTLDAKTVAIISFALCGFANFGSIGVVVGAFSAVAPHRAPEIAQLGL
                     RALAAATLSNLMSATIAGFFIGLA"
     gene            complement(6556..7644)
                     /locus_tag="ACHOSC_RS06950"
                     /old_locus_tag="pgaptmp_001390"
     CDS             complement(6556..7644)
                     /locus_tag="ACHOSC_RS06950"
                     /old_locus_tag="pgaptmp_001390"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar kinase"
                     /protein_id="HGU4547364.1"
                     /translation="MNDREKQILKILRRNPLIQQNEIADILQISRSRVAAHIMDLMRK
                     GLIKGKGYILTEQDYCVVVGAINMDIRGMADIRYPQAASHPGSVHCSAGGVGRNIAHN
                     LALLGRDVHLISAIGNDFYGETLLEETRRAGVNVSNCIRLHGHSTATYLAIANKQEET
                     ILAINDTHILQQLTPQLLNTSRDLIRHAGVVLADCNLTPEALEWVFTIADEIPMFVDT
                     VSEFKANKVKNWYSRIHTLKPTQNELEILWGQPIKDDNDRIRAVNSLHQQGVKRIFVY
                     LKDESVFCSDKDGEQFLLTAPAHTTVDSFGADDGFMAGLVYSFLEGYSFRDSARFAVA
                     CAAISRASGSLNNPTLSADNALSLVPMV"
     gene            complement(7648..8505)
                     /gene="nfo"
                     /locus_tag="ACHOSC_RS06955"
                     /old_locus_tag="pgaptmp_001391"
     CDS             complement(7648..8505)
                     /gene="nfo"
                     /locus_tag="ACHOSC_RS06955"
                     /old_locus_tag="pgaptmp_001391"
                     /EC_number="3.1.21.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribonuclease IV"
                     /protein_id="HGU4547365.1"
                     /translation="MKYIGAHVSAAGGLANAAIRAAEIDATAFALFTKNQRQWRAAPL
                     TTQTIDEFKAACEKYHYTSAQILPHDSYLINLGHPVTEALEKSRDAFIDEMQRCEQLG
                     LSLLNFHPGSHLMQISEEDCLARIAESINIALDKTQGVTAVIENTAGQGSNLGFKFEH
                     LAAIIDGVEDKSRVGVCIDTCHAFAAGYDLRTPAECEKTFADFARIVGFKYLRGMHLN
                     DAKSTFGSRVDRHHSLGEGNIGHDAFRWIMQDDRFDGIPLILETINPDIWAEEIAWLK
                     AQQTEKAVA"
     gene            complement(8579..9628)
                     /locus_tag="ACHOSC_RS06960"
                     /old_locus_tag="pgaptmp_001392"
     CDS             complement(8579..9628)
                     /locus_tag="ACHOSC_RS06960"
                     /old_locus_tag="pgaptmp_001392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YeiH family protein"
                     /protein_id="HGU4547366.1"
                     /translation="MTNITLQKQHRTLWHFIPGLALSAVITGVALWGGSIPAVAGAGF
                     SALTLAILLGMVLGNTIYPHIWKSCDGGVLFAKQYLLRLGIILYGFRLTFSQIADVGI
                     SGIIIDVLTLSSTFLLACFLGQKVFGLDKHTSWLIGAGSSICGAAAVLATEPVVKAEA
                     SKVTVAVATVVIFGTVAIFLYPAIYPLMSQWFSPETFGIYIGSTVHEVAQVVAAGHAI
                     SPDAENAAVISKMLRVMMLAPFLILLAARVKQLSGANSGEKSKITIPWFAILFIVVAI
                     FNSFHLLPQSVVNMLVTLDTFLLAMAMAALGLTTHVSALKKAGAKPLLMALVLFAWLI
                     VGGGAINYVIQSVIA"
     gene            9727..10608
                     /gene="yieE"
                     /locus_tag="ACHOSC_RS06965"
                     /old_locus_tag="pgaptmp_001393"
     CDS             9727..10608
                     /gene="yieE"
                     /locus_tag="ACHOSC_RS06965"
                     /old_locus_tag="pgaptmp_001393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator YeiE"
                     /protein_id="HGU4547367.1"
                     /translation="MHITLRQLEVFAEVLKSGSTTQASVMLALSQSAVSAALTDLEGQ
                     LGVQLFDRVGKRLVVNEHGRLLYPRALALLEQAVEIEQLFREDNGAIRIYASSTIGNY
                     ILPAVIARYRHDYPQLPIELSVGNSQDVMQAVLDFRVDIGFIEGPCHSTEIISEPWLE
                     DELVVFAAPTSPLARGPVTLEQLAAAPWILRERGSGTREIVDYLLLSHLPKFEMAMEL
                     GNSEAIKHAVRHGLGISCLSRRVIKDQLQAGTLSEVAVPLPRLMRTLWRIHHRQKHLS
                     NALRRFLDYCDPANVPR"
     gene            10813..12282
                     /gene="lysP"
                     /locus_tag="ACHOSC_RS06970"
                     /old_locus_tag="pgaptmp_001394"
     CDS             10813..12282
                     /gene="lysP"
                     /locus_tag="ACHOSC_RS06970"
                     /old_locus_tag="pgaptmp_001394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine-specific permease"
                     /protein_id="HGU4547368.1"
                     /translation="MVSETKTTEAPGLRRELKARHLTMIAIGGSIGTGLFVASGATIS
                     QAGPGGALLSYMLIGLMVYFLMTSLGELAAYMPVSGSFATYGQNYVEEGFGFALGWNY
                     WYNWAVTIAVDLVAAQLVMSWWFPDTPGWIWSALFLGVIFLLNYISVRGFGEAEYWFS
                     LIKVTTVIVFIIVGVLMIIGIFKGAQPAGWSNWTIGEAPFAGGFAAMIGVAMIVGFSF
                     QGTELIGIAAGESEDPAKNIPRAVRQVFWRILLFYVFAILIISLIIPYTDPSLLRNDV
                     KDISVSPFTLVFQHAGLLSAAAVMNAVILTAVLSAGNSGMYASTRMLYTLACDGKAPR
                     IFAKLSRGGVPRNALYATTVIAGLCFLTSMFGNQTVYLWLLNTSGMTGFIAWLGIAIS
                     HYRFRRGYVLQGHDINDLPYRSGFFPLGPIFAFILCLIITLGQNYEAFLKDTIDWGGV
                     AATYIGIPLFLIIWFGYKLIKGTHFVRYSEMKFPQNDKK"
     gene            12575..14566
                     /gene="cirA"
                     /locus_tag="ACHOSC_RS06975"
                     /old_locus_tag="pgaptmp_001395"
     CDS             12575..14566
                     /gene="cirA"
                     /locus_tag="ACHOSC_RS06975"
                     /old_locus_tag="pgaptmp_001395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="catecholate siderophore receptor CirA"
                     /protein_id="HGU4547369.1"
                     /translation="MFRLNPFVRVGLCLSAISCAWPVLAVDDDGETMVVTASSVEQNL
                     KDAPASISVITQEDLQRKPVQNLKDVLKEVPGVQLTNEGDNRKGVSIRGLDSSYTLIL
                     VDGKRVNSRNAVFRHNDFDLNWIPVDSIERIEVVRGPMSSLYGSDALGGVVNIITKKI
                     GQKWSGTVTVDTTIQEHRDRGDTYNGQFFTSGPLIDGVLGMKAYGSLAKREKDDPQNS
                     TTTDTGETPRIEGFSSRDGNVEFAWTPNQNHDFTAGYGFDRQDRDSDSLDKNRLERQN
                     YSVSHNGRWDYGTSELKYYGEKVENKNPGNSSPITSESNTVDGKYTLPLTAINQFLTV
                     GGEWRHDKLSDAVNLTGGTSSKTSASQYALFVEDEWRIFEPLALTTGVRMDDHETYGE
                     HWSPRAYLVYNATDTVTVKGGWATAFKAPSLLQLSPDWTSNSCRGACKIVGSPDLKPE
                     TSESWELGLYYMGEEGWLEGVESSVTVFRNDVKDRISISRTSDVNAAPGYQNFVGFET
                     GANGRRIPVFSYYNVNKARIQGVETELKIPFNDEWKLSINYTYNDGRDVSNGENKPLS
                     DLPFHTANGTLDWKPLALEDWSFYVSGHYTGQKRADSATAKTPGGYTIWNTGAAWQVT
                     KDVKLRAGVLNLGDKDLSRDDYSYNEDGRRYFMAVDYRF"
     gene            complement(14598..15434)
                     /gene="yeiG"
                     /locus_tag="ACHOSC_RS06980"
                     /old_locus_tag="pgaptmp_001396"
     CDS             complement(14598..15434)
                     /gene="yeiG"
                     /locus_tag="ACHOSC_RS06980"
                     /old_locus_tag="pgaptmp_001396"
                     /EC_number="3.1.2.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-formylglutathione hydrolase"
                     /protein_id="HGU4547370.1"
                     /translation="MEMLEEHRCFEGWQQRWRHDSSTLNCPMTFSIFLPPPRDHTPPP
                     VLYWLSGLTCNDENFTIKAGAQRVAAELGIVLVMPDTSPRGEQVANDDGYDLGQGAGF
                     YLNATQPPWATHYRMYDYLRDELPALVQSQFNVSDRCAISGHSMGGHGALIMALKNPG
                     KYTSVSAFAPIVNPCSVPWGIKAFSSYLGEDKNAWLEWDSCALMYASNAQDAIPTLID
                     QGDNDQFLADQLQPAVLAEAARQKAWPMTLRIQPGYDHSYYFIASFIEDHLRFHAQYL
                     LK"
     gene            15692..16360
                     /gene="folE"
                     /locus_tag="ACHOSC_RS06985"
                     /old_locus_tag="pgaptmp_001397"
     CDS             15692..16360
                     /gene="folE"
                     /locus_tag="ACHOSC_RS06985"
                     /old_locus_tag="pgaptmp_001397"
                     /EC_number="3.5.4.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003934 - GTP cyclohydrolase I activity
                     [Evidence IEA];
                     GO_process: GO:0046654 - tetrahydrofolate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP cyclohydrolase I FolE"
                     /protein_id="HGU4547371.1"
                     /translation="MPSLSKEAALVHEALVARGLETPLRPPVHEMDNETRKSLIAGHM
                     TEIMQLLNLDLADDSLMETPHRIAKMYVDEIFSGLDYANFPKITLIENKMKVDEMVTV
                     RDITLTSTCEHHFVTIDGKATVAYIPKDSVIGLSKINRIVQFFAQRPQVQERLTQQIL
                     IALQTLLGTNNVAVSIDAVHYCVKARGIRDATSATTTTSLGGLFKSSQNTRHEFLRAV
                     RHHN"
     gene            16377..17534
                     /gene="yeiB"
                     /locus_tag="ACHOSC_RS06990"
                     /old_locus_tag="pgaptmp_001398"
     CDS             16377..17534
                     /gene="yeiB"
                     /locus_tag="ACHOSC_RS06990"
                     /old_locus_tag="pgaptmp_001398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF418 domain-containing protein YeiB"
                     /protein_id="HGU4547372.1"
                     /translation="MERNVTLDFVRGVAILGILLLNISAFGLPKAAYLNPAWYGAITP
                     QDAWTWAFLDLIAQVKFLTLFALLFGAGLQMLLPRGRRWIQSRLTLLVLLGFIHGLLF
                     WDGDILLAYGLVGLICWWLVRDAPSVKSLFNTGVMLYLVGLGVLLLLGLISDSQTSRA
                     WTPDASAILYEKYWKLHGGVEAISNRADGVGNSLLALGAQYGWQLAGMMLIGAALMRS
                     GWLKGQFSLRHYRRTGFVLVAIGVTINLPAIALQWQLDWAYRWCAFLLQMPRELSAPF
                     QAIGYASLFYGFWPQLSRFKLVLAIACVGRMALTNYLLQTLICTMLFYHLGLFMHFDR
                     LELLAFVIPVWLANILFSVIWLCYFRQGPVEWLWRQLTLRAAGTAISKTSR"
     gene            17676..18716
                     /gene="galS"
                     /locus_tag="ACHOSC_RS06995"
                     /old_locus_tag="pgaptmp_001399"
     CDS             17676..18716
                     /gene="galS"
                     /locus_tag="ACHOSC_RS06995"
                     /old_locus_tag="pgaptmp_001399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708050.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator GalS"
                     /protein_id="HGU4547373.1"
                     /translation="MITIRDVARQAGVSVATVSRVLNNSTLVSADTREAVMKAVSELD
                     YRPNANAQALATQVSDTIGVVVMDVSDAFFGALVKAVDLVAQQHQKYVLIGNSYHEAE
                     KERHAIEVLIRQRCNALIVHSKALSDDELAQFMDNIPGMVLINRVVPGYAHRCVCLDN
                     LSGARMATRMLLNNGHQRIGYLSSSHGIEDDAMRKAGWMSALKEQDIIPPESWIGTGT
                     PDMPGGEAAMVELLGRNLQLTAVFAYNDNMAAGALTALKDNGIAIPLHLSIIGFDDIP
                     IARYTDPQLTTVRYPIASMAKLATELALQGAAGNIDPRASHCFMPTLVRRHSVATRQN
                     AATITNSTNQAM"
     gene            18996..19994
                     /gene="mglB"
                     /locus_tag="ACHOSC_RS07000"
                     /old_locus_tag="pgaptmp_001400"
     CDS             18996..19994
                     /gene="mglB"
                     /locus_tag="ACHOSC_RS07000"
                     /old_locus_tag="pgaptmp_001400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030246 - carbohydrate binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="galactose/glucose ABC transporter
                     substrate-binding protein MglB"
                     /protein_id="HGU4547374.1"
                     /translation="MNKKVLTLSAVMASMLFGAAAHAADTRIGVTIYKYDDNFMSVVR
                     KAIEQDAKAAPDVQLLMNDSQNDQSKQNDQIDVLLAKGVKALAINLVDPAAAGTVIEK
                     ARGQNVPVVFFNKEPSRKALDSYDKAYYVGTDSKESGIIQGDLIAKHWAANQGWDLNK
                     DGQIQFVLLKGEPGHPDAEARTTYVIKELNDKGIKTEQLQLDTAMWDTAQAKDKMDAW
                     LSGPNANKIEVVIANNDAMAMGAVEALKAHNKSSIPVFGVDALPEALALVKSGALAGT
                     VLNDANNQAKATFDLAKNLADGKGAADGTNWKIDNKVVRVPYVGVDKDNLAEFSKK"
     gene            20055..21575
                     /gene="mglA"
                     /locus_tag="ACHOSC_RS07005"
                     /old_locus_tag="pgaptmp_001401"
     CDS             20055..21575
                     /gene="mglA"
                     /locus_tag="ACHOSC_RS07005"
                     /old_locus_tag="pgaptmp_001401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020883197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0043211 - ABC-type carbohydrate
                     transporter activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="galactose/methyl galactoside ABC transporter
                     ATP-binding protein MglA"
                     /protein_id="HGU4547375.1"
                     /translation="MVSSTTPSSGEYLLEMSGINKSFPGVKALDNVNLKVRPHSIHAL
                     MGENGAGKSTLLKCLFGIYQKDSGTILFQGKEIDFHSAKEALENGISMVHQELNLVLQ
                     RSVMDNMWLGRYPTKGMFVDQDKMYRETKAIFDELDIDIDPRARVGTLSVSQMQMIEI
                     AKAFSYNAKIVIMDEPTSSLTEKEVNHLFTIIRKLKERGCGIVYISHKMEEIFQLCDE
                     VTVLRDGQWIATEPLAGLTMDKIIAMMVGRSLNQRFPDKENKPGEVILEVRNLTSLRQ
                     PSIRDVSFDLHKGEILGIAGLVGAKRTDIVETLFGIREKSAGTITLHGKQINNHNANE
                     AINHGFALVTEERRSTGIYAYLDISFNSLISNIRNYKNKVGLLDNSRMKSDTQWVIDS
                     MRVKTPGHRTQIGSLSGGNQQKVIIGRWLLTQPEILMLDEPTRGIDVGAKFEIYQLIA
                     ELAKKGKGIIIISSEMPELLGITDRILVMSNGLVSGIVDTKTTTQNEILRLASLHL"
     gene            21591..22601
                     /gene="mglC"
                     /locus_tag="ACHOSC_RS07010"
                     /old_locus_tag="pgaptmp_001402"
     CDS             21591..22601
                     /gene="mglC"
                     /locus_tag="ACHOSC_RS07010"
                     /old_locus_tag="pgaptmp_001402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="galactose/methyl galactoside ABC transporter
                     permease MglC"
                     /protein_id="HGU4547376.1"
                     /translation="MSALNKKSFLTYLKEGGIYVVLLVLLAIIIFQDPTFLSLLNLSN
                     ILTQSSVRIIIALGVAGLIVTQGTDLSAGRQVGLAAVVAATLLQSMDNANKVFPEMAT
                     MPIALVILIVCAIGAVIGLINGLIIAYLNVTPFITTLGTMIIVYGINSLYYDFVGASP
                     ISGFDSGFSTFAQGFVVLGSFRLSYITFYALIAVAFVWVLWNKTRFGKNIFAIGGNPE
                     AAKVSGVNVGLNLLMIYALSGVFYAFGGMLEAGRIGSATNNLGFMYELDAIAACVVGG
                     VSFSGGVGTVIGVVTGVIIFTVINYGLTYIGVNPYWQYIIKGAIIIFAVALDSLKYAR
                     KK"
     gene            complement(22672..23907)
                     /gene="preA"
                     /locus_tag="ACHOSC_RS07015"
                     /old_locus_tag="pgaptmp_001403"
     CDS             complement(22672..23907)
                     /gene="preA"
                     /locus_tag="ACHOSC_RS07015"
                     /old_locus_tag="pgaptmp_001403"
                     /EC_number="1.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416652.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0017113 - dihydropyrimidine dehydrogenase
                     (NADP+) activity [Evidence IEA];
                     GO_process: GO:0006210 - thymine catabolic process
                     [Evidence IEA];
                     GO_process: GO:0006212 - uracil catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent dihydropyrimidine dehydrogenase
                     subunit PreA"
                     /protein_id="HGU4547377.1"
                     /translation="MLTKDLSITFCGVKFPNPFCLSSSPVGNCYEMCAKAYDTGWGGV
                     VFKTIGFFIANEVSPRFDHLVKEDTGFIGFKNMEQIAEHPLEENLAALRQLKEDYPDK
                     VLIASIMGENEQQWEELARLVQEAGADMIECNFSCPQMTSHAMGSDVGQSPELVEKYC
                     RAVKRGSTLPMLAKMTPNIGDMCEVALAAKRGGADGIAAINTVKSITNIDLNQKIGMP
                     IVNGKSSISGYSGKAVKPIALRFIQQMRTHPELRDFPISGIGGIETWEDAAEFLLLGA
                     ATLQVTTGIMQYGYRIVEDMASGLSHYLADQGFDSLQEMVGLANNNIVPAEDLDRSYI
                     VYPRINLDKCVGCGRCYISCYDGGHQAMEWSEKTRTPHCNTEKCVGCLLCGHVCPVGC
                     IELGEVKFKKGEKEHPVTL"
     gene            complement(23901..25139)
                     /gene="preT"
                     /locus_tag="ACHOSC_RS07020"
                     /old_locus_tag="pgaptmp_001404"
     CDS             complement(23901..25139)
                     /gene="preT"
                     /locus_tag="ACHOSC_RS07020"
                     /old_locus_tag="pgaptmp_001404"
                     /EC_number="1.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent dihydropyrimidine dehydrogenase
                     subunit PreT"
                     /protein_id="HGU4547378.1"
                     /translation="MPQQNYLDELTPAFTPLLAIKEASRCLLCHDAPCSQACPAQTDP
                     GKFIRSIYFRNFKGAAETIRENNALGAVCARVCPTEKLCQSGCTRAGVDTPIDIGRLQ
                     RFVTDFEQQTGMEIYQPGTKTLGKVAIIGAGPAGLQASVTLTNQGYDVTIYEKEAHPG
                     GWLRNGIPQFRLPQSVLDAEIARIEKMGVTIKCNNEVGNTLTLEQLKAENRAVLVTVG
                     LSSGSGLSLFEHSDVEIAVDFLQRARQAQGDISIPQSALIIGGGDVAMDVASTLKVLG
                     CQAVTCVAREELDEFPASEKEFASARELGVSIIDGFTPVAVEGNKVTFKHVRLPGELT
                     MAADKIILAVGQHARLDAFAELEPQRNTIKTQNYQTRDPQVFAAGDIVEGDKTVVYAV
                     KTGKEAAEAIHHYLEGACSC"
BASE COUNT         6100 a         6103 c         6570 g         6434 t
ORIGIN      
        1 tgagcacatg tttaaatatg aacacatgtt taaactgtct ttgcagtatc tatattcttg
       61 tggattgaca gggttggtaa ttttttacca gaggacgtgc tatgcgcgaa aaggattatg
      121 tcgtaattat aggttcggcg aatattgatg tcgccggata ttcacatgaa tcattaaatt
      181 atgcggattc aaatccaggt aaaataaaat ttacacctgg tggagtaggg cgaaatattg
      241 cacaaaacct ggcgttgctg ggtaacaaag cctggttact gagcgcagta ggtagtgatt
      301 tttatggtca atcgctgcta acgcaaacca atcaatctgg cgtttatgtc gataaatgcc
      361 tgattgtgcc gggagaaaat acgtcgagtt atttatcatt actcgataat accggtgaaa
      421 tgctggttgc tataaatgac atgaatatta gcaacgctat tacagctgaa tatctcgcac
      481 agcaccgtga atttattcag agggcaaagg tcattgtcgc ggactgtaat atcagtgaag
      541 aggcactggc atggattctg gataatgctg ccaacgtacc cgtatttgtc gatccggttt
      601 cagcgtggaa atgtgtcaaa gtgcgcgacc gtctaaatca gatccacact ctgaagccaa
      661 accgccttga agcggaaaca ctgagtggga ttgcgctgtc agggcgtgga gatgtggcaa
      721 aagttgctgc ctggttccat caacatggcc tgaaccgact ggtattgagc atgggcggcg
      781 atggcgttta ttacagtgat atcagcggtg aaagtggctg gtctgccccg atcaaaacca
      841 atgttattaa tgttaccgga gcgggcgatg ccatgatggc gggacttgct tcgtgttggg
      901 tagacggaat gccgtttgcc gaatctgttc gtttcgcaca gggatgttcg tcaatggcgc
      961 tctcctgtga atacaccaat aaccccgatt tatcgattgc caacgttata tcgttagtgg
     1021 agaacgcaga atgtctgaat taaaaatttc ccctgaatta ttacaaattt ccccggaagt
     1081 gcaggacgct ttaaaaaaca aaaaaccggt tgtggcgctg gaatcgacca ttatttctca
     1141 cgggatgccg ttcccacaaa atgcccagac cgcaattgaa gtagaagaaa ctattcgtaa
     1201 acagggcgca gtacctgcca ctatcgccat tattggcggc gtgatgaaag tgggtttaag
     1261 caaagaagaa attgaattac tgggtcgtga agggcataac gtgaccaaag ttagtcgtcg
     1321 cgatttacct tttgttgttg ccgccggaaa aaatggcgca accactgtgg cttcaacgat
     1381 gattattgcg gcgcttgccg gaattaaagt atttgccacc gggggaattg gtggtgtgca
     1441 tcgcggggcg gaacatacct tcgatatttc tgccgatttg caagaactgg caaatactaa
     1501 tgtcaccgtt gtttgtgccg gggcgaaatc tattctcgat ttaggattaa caacagaata
     1561 tttagaaacc ttcggtgtgc cgttaattgg ctatcagaca aaagcgctgc ctgcgttttt
     1621 ctgccgtacc agcccatttg aagtcagtat tcgccttgat agtgccacgg aaatcgctcg
     1681 tgcaatggca gtgaaatggc aaaccggttt gaacggcggc ctggtagtgg cgaatccgat
     1741 cccggaacag tttgcgatgc cggaggaatc tatcaatgca gccatagatc aagccgtcgc
     1801 cgaagccgaa gagcagggcg ttattggtaa agaaagtaca ccgttcctgc tggcgcgcgt
     1861 tgctgagctg accggcggtg acagcctgaa atccaatatc cagctggtgt tcaacaacgc
     1921 cattctggcg agtgaaattg ccaaagaata ccagcgtctc gcgggttaat ttctgacggg
     1981 cagaaagccg tcgcccgtac tcatcaagca taccaaaacc tggcgctgac gccgggttta
     2041 ctggcatgaa gggaaaatca ttatggatat aatgagaagt gttgtgggga tggtggtgtt
     2101 actggcaata gcatttctgt tgtcagtgaa taaaaagagc atcagtttgc gcacggttgg
     2161 agccgcactg ctgctgcaaa tcgctattgg tggcatcatg ctctacttcc caccgggaaa
     2221 gtgggcagta gaacaggcgg cattaggcgt tcataaagtg atgtcttaca gtgatgccgg
     2281 tagcgccttc atttttggtt cgctggttgg gccgaaaatg gatgtcctgt ttgacggtgc
     2341 gggttttatc ttcgcctttc gcgtacttcc ggcgattatt ttcgttactg cgctcatcag
     2401 tctgctgtac tacattggcg tgatggggct gctgattcgc atccttggca gcattttcca
     2461 gaaagctctt aacatcagca aaatcgaatc ttttgtcgca gtcacaacta ttttcctcgg
     2521 gcaaaatgag atcccggcga tcgttaaacc gtttatcgat cgcatgaatc gcaacgagtt
     2581 gtttaccgca atttgtagcg ggatggcgtc cattgctggt tcgatgatga ttggttatgc
     2641 cggaatgggc gtaccaattg actacctgtt agcggcatcg ctgatggcga tcccaggtgg
     2701 tattttgttt gcacgtattc ttagcccggc caccgagcct tcacaggtca catttgaaaa
     2761 tctgtcgttc agcgaaacgc cgccaaaaag ctttatcgaa gcggcggcga gcggtgcgat
     2821 gaccgggctg aaaatcgccg ctggtgtagc gacggtggta atggcgtttg tcgcaattat
     2881 tgcgctgatc aacggcatta tcggcggaat tggtggctgg tttggtttcg ccaatgcctc
     2941 tctggaaagt atttttggct atgtgctggc accgctggcg tggatcatgg gtgtggactg
     3001 gagtgatgcc aatcttgcgg gtagcctgat tgggcagaaa ctggcgatta acgaattcgt
     3061 cgcttacctg agtttctccc catacctgca aacgggcggc acgctggaag tgaaaaccat
     3121 tgcgattatc tcctttgcgc tttgtggttt tgctaacttt ggttctatcg gtgttgtcgt
     3181 tggcgcattt tcggctattt cgccaaaacg cgcgccggaa atcgcccagc ttggtttacg
     3241 ggcgctggca gcagcaacgc tttccaacct gatgagtgcg accattgccg ggttctttat
     3301 tggtttagct tgagtttcat tgccggatgt acgcgcttat taagtgttgc atccggcaat
     3361 cgcgctattg caaataattt ttactgcatc atcccggaga atttattctc cgggtccatc
     3421 tccagcgcca gtcctgacaa ctgctttctg tttttaatta gatacccttt cttgctttta
     3481 attaataaac cgtcgtgaat gaattgcgcg agaacatata aaagatgtcg ataagaaacg
     3541 cctaaatact ctgccgcttg cgtatgcttt tcgtgataaa gatcaccttc ctgcgagagt
     3601 aatataaatg ctgccaggcg attcactaac ggaaatgatt gattctgagt taaagaaaca
     3661 atattacgat aatttttatg acttaaggtg acgcagagtt ttcgtaaaaa tagcgtgtcg
     3721 tttaataacc gcgggcggta atgtttcata gggagcgcaa ggcaccaaca ctcttcaata
     3781 gcctgcaccg cacgcggttc atggtctttg tcgattaact caatctcgcc aataaaacag
     3841 ggggcggcaa agaaatcgat cagcgacacg cgaccattag ctagcgtggc gtaaagcctg
     3901 gcgcggcctc gcgtcaggta aaacagccag gacggctgtt gcccttcctg cacaatgtag
     3961 tcacgcgcta aaaaatgaaa cagccgcgta tcggctgaaa catccgttaa aaagcaatcc
     4021 ttaaacgcgg attcactcat caattgctgc ttaagatcat tattgtggat ttctttcacg
     4081 atgtttatcc gctatgagat ttctcatatt tagcatacgt acgcggttgg taagattcaa
     4141 tcatcggccc ggtcgaattt tctttgctgt agaaaaaata acgcaactgg aaacagagga
     4201 aataaacaat ggaaaagaga aaaattattc tggattgtga tccgggtcat gatgatgcta
     4261 ttgcaatgat gatggcagcg aaacatccgg caatagattt attaggcatc actattgtag
     4321 cgggtaatca gacgcttgat aaaacattaa ttaatggcct gaatgtttgc cagaaactgg
     4381 agattaatgt tccggtttac gcggggatgc cgcaacccat tatgcgtcaa caaatcgttg
     4441 ccgataatat tcacggtgaa accggactgg atggcccggt attcgagccg ctaacccgcc
     4501 aggcagaaag tacccacgcg gtgaaataca tcattgatac cctgatggca agcgatggcg
     4561 atatcactct ggtgccggtt ggtccgcttt caaatatcgc ggtggcaatg cgtatgcaac
     4621 ccgcgatcct gcccaaaatc cgtgaaattg tgctaatggg cggcgcttac ggtacaggca
     4681 acttcacgcc atctgccgag ttcaacatct ttgctgaccc ggaagcagcc cgcgtggtgt
     4741 tcacctctgg cgttccatta gtgatgatgg gcctcgatct caccaaccag accgtttgca
     4801 ccccggacgt gattgctcgg atggaaaggg caggcggccc tgccggagag ctgttcagcg
     4861 acatcatgaa cttcactctc aaaacgcagt tcgaaaacta cggcctggct ggcggcccgg
     4921 tgcacgacgc cacctgcatc ggttatctga ttaaccctga tggcattaaa acccaggaga
     4981 tgtacgtcga agtggacgtc aacagtggcc cttgctatgg gcgcaccgtc tgcgacgagc
     5041 tgggcgttct tggcaagccc gccaatacca aagtcggcat cactattgat accgactggt
     5101 tctggggctt agtcgaagag tgcgtgcgcg gctacatcaa aacccattaa gcgttgataa
     5161 caagccgggc cgatagcgca agatgcaggc ccggctattt ctgatgggca gaaagccgtc
     5221 gcccaatatg acatgaagag ataatcacta tggatgtcat gagaagtgtt ctgggaatgg
     5281 tggtattgct ggcaattgca tttttgctgt cagtaaacaa gaagaagatc agcctgcgta
     5341 ccgttggcgc ggcgttagtg ttacaggttg tgattggcgg cattatgctt tggttaccgc
     5401 cagggcgttg ggtcgctgaa aaagtcgctt ttggcgtgca taaagtgatg gcgtacagcg
     5461 acgcgggtag cgcatttatc ttcggttcgc tggtcgggcc gaaaatggac acgctgtttg
     5521 atggcgcagg atttatcttt ggtttcagag tattaccggc aattatcttc gttacagcac
     5581 tggtgagtat tctctactac atcggtgtga tggggatttt aattcgcatt ctcggcggta
     5641 tcttccagaa agcgttaaat atcagcaaga tcgagtcatt cgtcgcggtc actaccattt
     5701 tcctcgggca aaacgaaatt ccggcaatcg tcaaaccctt tatcgatcgt ctgaatcgca
     5761 atgaattatt tacagcgatt tgtagtggca tggcctcgat tgctggttcg acaatgattg
     5821 gttacgccgc cctgggcgta cctgtggaat atttgctggc ggcatcgtta atggcgatcc
     5881 caggcgggat cttgtttgcc cgcctgttaa gcccggctac ggaatcttcg caggtttcct
     5941 ttaataacct ctctttcacc gaaacaccgc caaaaagcat tattgaagcc gctgcgacag
     6001 gggcaatgac cgggctgaaa atcgccgccg gtgtagcgac agttgttatg gcatttgtcg
     6061 ccatcattgc gttaattaat ggtattatcg gcggcgttgg cggctggttt ggttttgcac
     6121 atgcctcgct ggagtccatt ttaggttacc tgttggcccc attggcgtgg gtgatggggg
     6181 ttgactggag tgatgcaaat cttgccggga gtttgattgg gcagaagctg gcgatcaatg
     6241 aatttgtcgc ttatctcaat ttctcgccat atctgcaaac gggtggcact ctggatgcta
     6301 aaaccgtggc gattatttct ttcgcgttgt gcggtttcgc taactttggt tctatcgggg
     6361 tggtggtggg ggcgttttct gcggttgcgc cacaccgtgc gccggaaatc gcccaacttg
     6421 gtttacgcgc gctggcggcg gcgacacttt ctaacctgat gagtgctact attgcaggat
     6481 tctttattgg tttagcgtag tgcgcaattt atgagccgga taagatgcgg tcaagcgtcg
     6541 aatccggcaa cattgttaca ccattggcac taatgaaagc gcgttatcgg cagacagggt
     6601 agggttgttt aagctgccgc tggcgcgcga aattgccgcg caggctaccg caaaacgggc
     6661 gctgtcgcgg aaactgtatc cttcaagaaa gctatatacc aggcccgcca taaaaccatc
     6721 gtcagcacca aaactgtcta ctgtcgtatg cgctggcgcg gtcagcaaaa attgttctcc
     6781 atccttatcg ctgcaaaata cagattcatc tttcagataa acaaaaatgc gtttaacacc
     6841 ttgttgatgc agcgaattta cagctctgat gcgatcgttg tcgtctttaa tcggttgccc
     6901 ccacaaaatt tccagttcat tttgtgttgg tttcagagtg tggatccggc tgtaccagtt
     6961 ttttactttg ttcgctttaa attcggaaac ggtatcgaca aacatcggaa tttcatcagc
     7021 aatggtaaag acccactcca gggcttcagg tgtgagatta caatcagcca gcaccacacc
     7081 agcatggcga atcaagtccc gtgaagtatt gagcagttga ggcgttagct gttgcagaat
     7141 atgtgtgtca ttgattgcca ggatagtttc ttcctgctta ttagcgatag cgagataagt
     7201 tgcagtgctg tgtccatgca gacgaatgca gtttgaaacg ttcacgcccg cgcggcgcgt
     7261 ttcttccagt aatgtctcgc cgtaaaaatc gttgccaata gctgatatta aatgcacatc
     7321 ccgaccaagc agagccaggt tatgcgcgat attacgtccg acgccgcctg cggaacagtg
     7381 aacagaacct ggatgagagg ctgcttgcgg gtaacggata tccgccattc cgcgaatatc
     7441 catattgatt gcacccacaa ccacacagta atcctgctcg gtaaggatat atcctttgcc
     7501 tttaatcaaa cccttgcgca tcaaatccat aatatgagcg gcaacacgtg agcggctgat
     7561 ttgcagaata tcggcgattt cattctgttg aatcagcggg ttacgccgta aaattttaag
     7621 aatctgcttt tccctgtcgt tcatcgatca ggccaccgct ttttcagttt gttgcgcttt
     7681 cagccaggcg atctcttctg cccagatatc cgggttgatg gtttcaagga tcagcggaat
     7741 gccgtcgaaa cggtcgtcct gcatgatcca gcggaacgca tcatgaccga tattgccttc
     7801 accgaggcta tgatggcggt caacgcggct gccaaaggtg cttttcgcat cgttaaggtg
     7861 catcccgcgc agatacttaa agccaacaat acgggcaaaa tcagcgaacg ttttttcgca
     7921 ttcggctgga gtacgcaaat catacccggc agcgaaagca tggcaggtat caatgcagac
     7981 gccgacacgg gatttatctt ccacgccgtc gataatcgcc gcgagatgtt cgaatttaaa
     8041 ccctaagtta ctgccctgac cggcggtgtt ttcaatcacc gctgttacac cttgagtttt
     8101 atccagcgca atgttgatgg attcggcaat acgcgcaagg caatcctctt ctgaaatctg
     8161 catcagatgg ctgccagggt ggaagttgag caaagaaagc cccagttgtt cgcaacgctg
     8221 catttcatct ataaaggcat cgcgcgattt ttccagagct tcagtgaccg gatgtccgag
     8281 gttaatcaga tagctgtcgt ggggaagaat ttgcgccgat gtgtagtgat atttttcaca
     8341 ggccgctttg aattcatcga tggtttgcgt cgtgagcggt gcggcacgcc actgacgttg
     8401 gtttttggtg aacaaggcaa acgcggttgc gtcgatttcg gcggcgcgaa ttgcggcatt
     8461 tgccagaccg ccagcagcac taacgtgcgc tccaatgtat ttcatgcgag gactcctgtt
     8521 aaacccgctg gaggaaaacg gtaatgatag cgggttaaca ggagcaagat gtagtggttt
     8581 atgcgatgac gctttgaatc acatagttaa tcgcaccacc accaacaatc agccaggcaa
     8641 acagtaccag tgccatcagc agaggtttcg ccccggcttt tttcagcgcg ctgacgtgag
     8701 tggtcagacc cagcgccgcc atcgccattg ccagcaggaa ggtatccagc gttaccagca
     8761 tgttcaccac gctctgcggt aacaggtgga acgagttaaa gatggcaact acgatgaaca
     8821 agatggcaaa ccacggaata gtgattttgc ttttctcgcc gctgttcgcc ccagacagct
     8881 gtttaacacg cgccgccagc aggatgagga acggagccag catcatcacg cgcagcattt
     8941 tggaaataac tgctgcgttt tccgcatccg ggctgatggc atgacctgcc gccaccacct
     9001 gcgccacttc gtgcacagta gaaccaatgt agataccgaa agtttccgga ctaaaccatt
     9061 gagacatcag cggatatatc gccgggtaga ggaaaatagc gacggtcccg aagataacaa
     9121 cggttgcaac agccacggtt actttactgg cttccgcttt cactaccggc tcagtcgcca
     9181 gtaccgcggc agcaccacag atactgctac cggcaccgat caaccagctg gtgtgcttat
     9241 ccagaccaaa cactttctgc cccaggaagc aagccagcag gaaggtactg gacagcgtca
     9301 acacgtcaat gatgatccca ctgataccga catcggcaat ttgcgagaac gtcagacgga
     9361 agccataaag aatgataccc agacgtaata aatattgctt ggcaaacagc acaccaccgt
     9421 cacagctttt ccagatgtgc ggatagatgg tgttgcctaa aaccatcccc aacaagattg
     9481 cgagggtgag ggcactaaac ccggcacccg caaccgcggg aatggaacca ccccacaggg
     9541 cgaccccggt gataactgca ctcagggcta accccggaat aaaatgccac agtgtacgat
     9601 gttgtttctg taaggtgata ttcgtcataa ccctctcctt taaccgggca taaggttacg
     9661 actaactggt ttaaaaataa aattgattat atatttataa ttaatcttta taagtggtaa
     9721 gcgactatgc acatcaccct ccggcagttg gaagtttttg cagaagtatt gaaaagtgga
     9781 tcaaccaccc aggcgtcggt gatgctggcg ttgtcgcaat cagcagtgag cgcagccttg
     9841 accgacctgg aagggcagct tggcgtgcaa ctgtttgatc gcgtggggaa aagactggtt
     9901 gttaatgaac acgggcggct gctctatccg cgtgcgttgg cattgcttga acaggcggtt
     9961 gaaatcgaac aactgtttcg cgaagacaac ggcgcgattc gtatctatgc cagtagtacc
    10021 atcggtaact acattctgcc tgcagttatc gcccgttatc gccatgatta tccgcagttg
    10081 ccgattgaac ttagcgttgg gaacagccag gacgtgatgc aagcggtgct ggatttccgc
    10141 gttgatattg gctttattga aggaccgtgc cacagcactg aaatcatttc tgaaccgtgg
    10201 ctggaagacg agctggtggt tttcgccgcg ccgacttcgc cgttggctcg tggtccggtc
    10261 accttagaac agctggccgc tgcgccgtgg atcctgcgtg aacgcggttc cggcacgcgg
    10321 gagattgtcg attatctgtt gctgtcacat ttaccgaagt ttgagatggc gatggaatta
    10381 ggtaactccg aggcaatcaa acatgcggtg cgtcatgggt tgggaattag ttgcctgtcg
    10441 cgacgggtga ttaaagatca attgcaggca ggcacattaa gtgaagttgc ggtccctctc
    10501 ccgcgcctga tgcgtacgtt gtggcgtata catcatcggc aaaaacacct ttccaacgcg
    10561 ctacggcgct ttctggacta ttgcgatccc gcgaatgtac cgcgttaagt tgctgtacaa
    10621 gaacatgctg gtgctgtgtc gatttcgtga cgcagcgccc tcagcatgca ttcgccagaa
    10681 aagagattgg ctgctttact tataatccct gggcgatcat gaaggtgtct tataaccgtg
    10741 tatttctgcc ggaaggattg ccaatcgtct gctacaatcg cgcctcattt ttaagatgga
    10801 tagcattttt gtatggtttc cgaaactaaa accacagaag cgccgggctt acgccgtgaa
    10861 ttaaaggcgc gtcacctgac gatgattgcc attggcggtt ccatcggtac aggtcttttt
    10921 gttgcctctg gcgcaacgat ttctcaggca ggtccgggcg gggcattgct ctcgtatatg
    10981 ctgattggcc tgatggttta cttcctgatg accagtctcg gtgaactagc tgcatatatg
    11041 ccggtttccg gttcgtttgc cacttacggt cagaactatg ttgaagaagg ctttggcttc
    11101 gcgctgggct ggaactactg gtacaactgg gcggtgacta tcgccgttga cctggttgca
    11161 gctcagctgg tcatgagctg gtggttcccg gatacaccgg gctggatctg gagtgcgttg
    11221 ttcctcggcg ttatcttcct gctgaactac atctcagttc gtggctttgg tgaagcggaa
    11281 tactggttct cactgatcaa agtcacgaca gttattgtct ttatcatcgt tggcgtgctg
    11341 atgattatcg gtatcttcaa aggcgcgcag cctgcgggct ggagcaactg gacaatcggc
    11401 gaagcgccgt ttgctggtgg ttttgcggcg atgatcggcg tagctatgat tgtcggcttc
    11461 tctttccagg gaaccgagct gatcggtatt gctgcaggcg agtccgaaga tccggcgaaa
    11521 aacattccac gcgcggtacg tcaggtgttc tggcgaatcc tgttgttcta tgtgttcgcg
    11581 atcctgatta tcagcctgat tattccgtac accgatccga gcctgctgcg taacgatgtt
    11641 aaagacatca gcgttagtcc gttcaccctg gtgttccagc acgcgggtct gctctctgcg
    11701 gcggcggtga tgaacgcagt tattctgacg gcggtgctgt cagcgggtaa ctccggtatg
    11761 tatgcgtcta ctcgtatgct gtacaccctg gcgtgtgacg gtaaagcgcc gcgcattttc
    11821 gctaaactgt cgcgtggtgg cgtgccgcgt aatgcgctgt atgcgacgac ggtgattgcc
    11881 ggtctgtgct tcctgacctc catgtttggc aaccagacgg tatacctgtg gctgctgaac
    11941 acctccggga tgacgggttt tatcgcctgg ctggggattg ccattagcca ctatcgcttc
    12001 cgtcgcggtt acgtattgca gggacacgac attaacgatc tgccgtaccg ttcaggtttc
    12061 ttcccactgg ggccgatctt cgcattcatt ctgtgtctga ttatcacttt gggccagaac
    12121 tacgaagcgt tcctgaaaga taccattgac tggggcggcg tagcggcaac gtatattggt
    12181 atcccgctgt tcctgattat ttggttcggc tacaagctga ttaaaggaac tcacttcgta
    12241 cgctacagcg aaatgaagtt cccgcagaac gataagaaat aagtttcctc ccttccttgc
    12301 taagccctct caaccgagag ggctttttca attccatttc cctgacaaat catgcggata
    12361 taaaatttaa catttggatt gataattgtt atcgtttgca ttatcgttac gccgcaatca
    12421 aaaaaggctg acaaatcaga ggctgttccg gctttctggg atgatcacct gcatacaaat
    12481 aagtccaccg cgatgctgcc gtacgcaagg ggacgtgaag aagatgtgag cgataaccca
    12541 ttttattttc gtagttacct catggagata tggaatgttt aggttgaacc ctttcgtacg
    12601 ggtcgggctg tgtttgtccg ctatttcttg tgcatggcct gtgttagcgg tcgatgatga
    12661 tggcgaaacg atggttgtca ctgcatcttc cgttgaacaa aacctcaaag atgcacccgc
    12721 cagtatcagc gtcattaccc aggaagacct gcagcgaaaa ccggtacaga atctgaagga
    12781 tgtcctcaaa gaagtgcctg gcgtacaact gacgaacgaa ggggataacc gtaagggcgt
    12841 tagtattcgt ggtctggaca gcagctacac cctgattctc gtcgacggta aacgcgttaa
    12901 ctcccgcaat gccgtcttcc gccacaatga tttcgatctg aactggatcc cggtcgattc
    12961 catcgaacgt attgaagtgg ttcgtggccc gatgtcgtcg ctatacggct ccgatgcgct
    13021 cggcggtgta gtgaatatca tcaccaaaaa aatcggtcag aaatggtcgg gtaccgttac
    13081 cgtcgatacc accattcagg aacatcgcga tcgcggtgat acctataacg gtcagttctt
    13141 taccagtgga ccattaattg atggtgtgct gggaatgaaa gcttacggca gcctggcaaa
    13201 acgtgaaaag gatgacccgc aaaactcaac gaccaccgat accggagaaa cgccgcgtat
    13261 tgaaggattc tccagccgcg acggcaatgt cgaatttgcc tggacaccga atcaaaatca
    13321 cgattttact gccggatacg gtttcgaccg tcaggatcgt gattccgact cgctggacaa
    13381 aaaccgcctg gaacgccaga actactccgt cagccataat gggcgttggg attacggcac
    13441 cagcgaactg aaatactacg gtgagaaagt cgagaacaaa aaccctggca acagcagccc
    13501 gataacttcc gaaagcaata cggtcgacgg caaatacacg ttgccgctga cggcaattaa
    13561 tcagtttctc acggttggcg gtgaatggcg tcacgacaaa cttagcgatg cggtgaacct
    13621 gaccggggga accagctcca aaacgtctgc cagccagtac gcgctgtttg tggaagatga
    13681 atggcggatc ttcgagccgc tggcgctgac gaccggcgtg cgtatggacg atcacgaaac
    13741 ctacggtgaa cactggagtc cgcgtgccta cctggtttat aacgccaccg acaccgtaac
    13801 ggtgaaaggg ggctgggcga cggcatttaa agcaccttct ctgttgcaac ttagccctga
    13861 ctggacgagc aattcctgcc gtggcgcatg taagattgtg ggtagcccgg atctgaaacc
    13921 agaaaccagc gaaagttggg agctggggct ttactacatg ggtgaagaag gctggctgga
    13981 aggggttgaa tccagcgtta ccgttttccg taacgatgtg aaagatcgta tcagcatcag
    14041 ccgtacgtct gacgtcaacg ctgcaccggg ctaccaaaac tttgttggtt ttgagacggg
    14101 cgctaacgga cggcgcatac cggtatttag ctactacaac gttaacaaag ctcgtattca
    14161 gggcgtggaa accgaactga aaattccgtt caacgatgaa tggaaactgt cgatcaacta
    14221 cacctacaac gatggtcgtg atgtcagcaa cggcgaaaac aaaccgctat ccgatctgcc
    14281 gttccatact gctaacggta cgctggactg gaaaccgctg gcgctggaag actggtcatt
    14341 ctatgtttct ggtcactata ccgggcagaa acgcgccgac agcgcgacgg ctaaaacacc
    14401 gggcggttat accatctgga ataccggcgc ggcctggcag gtgactaaag acgtcaaact
    14461 gcgcgcaggc gtgctgaacc ttggcgacaa ggatctcagt cgtgacgact acagctataa
    14521 cgaagacgga cgtcgttact ttatggcagt ggattatcgc ttctgatgag aagatgcccg
    14581 gcgaaccggg cggactttca cttcagtaaa tactgcgcat ggaagcgcag gtgatcctct
    14641 ataaaagagg cgatgaagta gtaactgtga tcatatcccg gctgaatacg cagcgtcatc
    14701 ggccacgctt tctggcgcgc ggcttctgcc agtaccgcag gttgcaactg gtcggcaaga
    14761 aactgatcat tatcgccctg atcgataagc gtcgggatcg catcctgcgc gttactggca
    14821 tacatcagcg cgcaactgtc ccattccagc catgcatttt tgtcctcacc taaatagctg
    14881 ctaaacgctt tgattcccca cgggacgctg cacggattca caattggcgc aaaggccgaa
    14941 acgctggtgt atttacccgg atttttcagc gccataatca gcgcaccgtg accacccatt
    15001 gaatgaccgc taatggcgca gcggtcgctg acattaaatt gcgactgaac cagcgccggt
    15061 aattcatcgc gcagataatc atacatccgg taatgcgtcg cccacggcgg ttgcgtggcg
    15121 ttaagataaa accccgcgcc ctggcctaaa tcgtagccat catcgttggc aacctgttcg
    15181 ccgcgcgggc tggtgtctgg catcaccagt acaatcccca gttctgccgc cacgcgctgg
    15241 gcacccgcct tgatggtgaa gttctcgtca ttgcaggtta atccggaaag ccagtacagc
    15301 actggtggcg gggtgtgatc acgaggtggt gggagaaaga tactgaacgt catcggacag
    15361 tttaaggtac tggagtcgtg tcgccagcgt tgctgccagc cttcaaaaca gcggtgctct
    15421 tcgagcattt ccatgcgtgg ctccttgttg tgttgtttgc aatttcttca tcataccgtt
    15481 tattcaatgt gctgtgagta actttcactt ccgtatttgc ataacgatgt tttaacatct
    15541 gctgatgaaa ggcagcggca attacaataa ttatcgctgt gaatactgga ttatgtgcgc
    15601 cgcctcacgc acaataatca agctgtaaat cagcttaata actttgcccc cacgcagggc
    15661 ggaggcgtca cacctgcagg agaaatcata aatgccatca ctcagtaaag aagcggccct
    15721 ggttcatgaa gcgttagttg cgcgaggact ggaaacaccg ctgcgcccgc ccgtgcatga
    15781 aatggataac gaaacgcgca aaagccttat tgctggtcat atgaccgaaa tcatgcagct
    15841 gctgaatctc gacctggctg atgacagttt gatggaaacg ccgcatcgca tcgctaaaat
    15901 gtatgtcgat gaaattttct ccggtctgga ttacgccaac ttcccgaaaa tcaccctcat
    15961 tgaaaacaaa atgaaggtcg atgaaatggt caccgtgcgc gatatcactc tgaccagtac
    16021 ctgtgaacac cattttgtta ccatcgatgg caaagcgacg gtggcttata tcccgaaaga
    16081 ttcggtgatc ggtctgtcaa aaattaaccg tatcgtgcag ttctttgccc agcgtccgca
    16141 ggtgcaggaa cgtctgacgc agcaaattct tattgcgcta caaacgctgc tgggcaccaa
    16201 taacgtggct gtctcgatcg acgcggtgca ttactgcgtg aaggcgcgtg gcatccgcga
    16261 tgcaaccagt gccacaacaa cgacctctct tggtggattg ttcaaatcca gtcagaatac
    16321 gcgccacgag tttctgcgcg ctgtgcgtca tcacaactga ttaaaaggca ggaaccatgg
    16381 agcgcaacgt cacgctcgat tttgttcgcg gcgtcgccat tctggggatc ctgctattaa
    16441 acatcagcgc ctttgggcta ccaaaggcgg cttatctcaa tcccgcctgg tacggtgcta
    16501 ttacgccgca ggatgcatgg acctgggcat ttcttgatct catcgcccag gtgaaattcc
    16561 tcacgctttt tgcgctgctg tttggtgcag gactgcaaat gttgctgccc cgtggcagac
    16621 gctggatcca gtcgcggtta acgctgttag tcttattggg ctttattcac ggtttattgt
    16681 tctgggacgg cgatattctg ctggcttacg ggctggtggg cttaatctgc tggtggctgg
    16741 tgcgcgatgc gccatcggta aaaagcctgt ttaatacagg cgtcatgctt tatctggtgg
    16801 ggcttggcgt tttgctgtta ttggggctga tttccgacag ccagaccagc cgcgcctgga
    16861 cgccggatgc atcggctatt ttgtatgaaa aatactggaa gcttcacggc ggcgttgaag
    16921 cgatcagtaa tcgtgccgat ggtgttggca acagtttact ggcactgggc gcacagtatg
    16981 gctggcaact ggctgggatg atgctcattg gtgcagcgct gatgcgcagc ggctggctga
    17041 aagggcagtt cagcttacgt cactatcgtc gtactggttt tgtgctggtg gcgattgggg
    17101 tgaccattaa ccttcctgcc atcgccctgc aatggcagct ggactgggcg tatcgctggt
    17161 gtgccttctt acttcaaatg ccgcgggaac tgagtgcgcc gtttcaggcg attggctatg
    17221 cgtcgctgtt ttatggcttc tggccgcaat tgagccgctt taagctggtg cttgcgatcg
    17281 cctgcgtcgg acggatggcg ctgaccaact atctattgca aacgctgatt tgtaccatgc
    17341 ttttttacca cctcggtttg tttatgcatt ttgaccgcct ggagctgctg gcgtttgtta
    17401 ttccggtatg gctggcgaat atcctcttct ctgttatctg gctgtgttac ttccgccagg
    17461 ggccggtgga atggctctgg cgtcagttaa ctttgcgtgc tgccggaacg gcaatatcta
    17521 aaacatcaag ataacgatct ggatcacatt cgttaacaaa acggctgtaa ccgtttccat
    17581 tgctgtgact cgattcacga agtcctgtat tcagtgctga caaaatagcc gccagcaagc
    17641 agtcatttac tgcaatctca taacaggtag tgaatatgat caccattcgt gatgtagcgc
    17701 gtcaggctgg cgtctctgtg gcaacggttt cccgggtgct caataacagc acgctggtca
    17761 gtgccgacac gcgtgaagca gtaatgaaag ccgtgagtga gctggattat cggccaaacg
    17821 ccaatgccca ggcgctggca actcaggtta gcgacaccat tggcgtggtg gtgatggacg
    17881 tttctgatgc gtttttcggc gcgctggtaa aagcggtgga tctggtcgct cagcagcatc
    17941 agaaatacgt gctaatcggc aatagctatc atgaagcgga aaaagagcgt cacgccattg
    18001 aggtgttaat tcgccagcgt tgtaatgcgt tgattgttca ctcaaaagca ttgagtgacg
    18061 atgaactggc gcaatttatg gataacattc ccggtatggt gttaatcaac cgcgttgtgc
    18121 cggggtacgc ccatcgttgc gtttgcctgg ataatctcag cggtgcccga atggcgacgc
    18181 gcatgttgct gaataacggt catcaacgta ttggttatct ttcttccagt cacggcattg
    18241 aagatgacgc catgcgtaaa gcaggctgga tgagtgcgtt gaaagagcag gatattattc
    18301 cgccggaaag ctggattggc actggtacgc cggacatgcc gggcggtgag gcggcgatgg
    18361 ttgaactgct ggggcgcaat ctacaactta ccgctgtatt tgcttataac gacaatatgg
    18421 ctgctggcgc actgacagca ttaaaagata atggcattgc gattccgtta catctctcaa
    18481 tcatcggttt cgatgatatt cccatcgccc gttacaccga cccgcaatta acgaccgtgc
    18541 gttatcccat tgcttcaatg gcgaaattag ccaccgaact ggccttgcag ggggcagcag
    18601 gcaatattga tcctcgtgct agccactgtt ttatgccgac gttagtgcgt cgccattctg
    18661 tcgcaacacg ccagaatgcg gcgacgatca ctaactcaac aaatcaggcg atgtaaccgc
    18721 tttcaatctg tgagtgattt cacagtatct taacaatgtg atagctatga ttgcaccgtt
    18781 ttaacgttgt aacccgtatg taacagtgaa taatcacttt caccgaggta atagcgtcat
    18841 aacaacaatt aaagccgttt tctggagcgt taccgggcat ggaagaacga attttaaaaa
    18901 gtgagcttcg gcgttcagta acacttcatt aactctactg ccccgccgag catttatctc
    18961 aagcactacc ctgcataaga aaaaccggag ataccatgaa taagaaggtg ttaaccctgt
    19021 ctgctgtgat ggccagcatg ttattcggtg ccgctgcaca cgctgctgat actcgcattg
    19081 gtgtaacaat ctataagtac gacgataact ttatgtctgt agtgcgcaag gctattgagc
    19141 aagatgcgaa agccgcgcca gatgttcagc tgctgatgaa tgattctcag aatgaccagt
    19201 ccaagcagaa cgatcagatc gacgtattgc tggcgaaagg ggtgaaggca ctggcaatca
    19261 acctggttga cccggcagct gcgggtacgg tgattgagaa agcgcgtggg caaaacgtgc
    19321 cggtggtttt cttcaacaaa gaaccgtctc gtaaggcgct ggatagctac gacaaagcct
    19381 actacgttgg cactgattcc aaagagtccg gcattattca gggcgatttg attgctaaac
    19441 actgggcggc gaatcagggt tgggatctga acaaagacgg tcagattcaa ttcgtattgc
    19501 tgaaaggtga accgggccat ccggatgcag aagcacgtac cacttacgtg attaaagagc
    19561 tgaacgacaa aggcattaaa actgaacagt tacagttaga taccgctatg tgggataccg
    19621 ctcaggcgaa agataagatg gacgcctggc tgtctggccc gaacgccaac aaaatcgaag
    19681 tggttatcgc caacaacgat gcgatggcaa tgggcgcggt agaagcactg aaagcacaca
    19741 acaagtccag cattccggtg tttggcgtcg atgcgctgcc agaagcgctg gcgctggtga
    19801 aatccggtgc actggcgggc acagtactga acgatgctaa caaccaggcg aaagcgacct
    19861 ttgatctggc gaaaaacctg gccgatggca aaggtgcggc tgatggcacc aactggaaaa
    19921 tcgacaacaa agtggtccgc gtaccttatg ttggcgtaga taaagacaac ctggctgaat
    19981 tcagcaagaa ataagactga ttgggcgcag tcgtttactg cgcccttatt gtatggccaa
    20041 caaggtaaaa ttatatggtc agctcaacga ctccgtcttc cggggaatac ttgttggaaa
    20101 tgagcggtat caacaagtcc tttcctggtg ttaaggcact tgataacgtt aatttaaaag
    20161 tccggccaca ttctatccat gcattaatgg gggaaaacgg tgcaggaaaa tcgacattat
    20221 taaaatgcct gtttggtatt tatcaaaaag actccggcac cattttattc cagggtaaag
    20281 agatcgattt ccattccgcc aaagaggcac tggaaaatgg tatttcgatg gtacaccagg
    20341 agttaaacct ggtattacaa cgttcggtga tggataacat gtggctgggg cgatatccca
    20401 ccaaaggcat gtttgtcgat caggacaaaa tgtaccgcga aaccaaagcg atttttgatg
    20461 aactggatat tgatatcgat ccgcgtgcgc gcgtcggcac attatccgtt tcgcaaatgc
    20521 agatgatcga aatcgccaaa gcgttttcct ataacgcgaa aattgtgatt atggatgaac
    20581 cgacttcttc gttaaccgaa aaagaggtca atcatctgtt cactattatt cgtaaattaa
    20641 aagagcgcgg ctgcggtatt gtttatatct cgcataaaat ggaagaaatc ttccagttat
    20701 gtgatgaagt taccgtattg cgcgacggtc agtggatcgc caccgaaccg ctggcaggac
    20761 tgacgatgga caagatcatc gccatgatgg ttgggcgttc tcttaatcag cgttttcctg
    20821 acaaagaaaa caagccgggc gaagtcatcc tcgaggtgcg taacctaacg tcactgcgcc
    20881 agccgtcgat tcgcgatgtc tcgtttgatc tgcataaagg ggagatcctc ggtattgccg
    20941 ggctggtggg ggcgaaacgt accgatattg ttgagacgtt atttggtatt cgcgagaaat
    21001 cggctggcac cattacgttg cacggcaaac agatcaataa ccataatgcc aacgaagcca
    21061 taaaccacgg atttgcactg gtaactgagg agcgccgctc aacgggaatt tatgcctatc
    21121 tggatattag ttttaactcg ttaatttcca atattcgcaa ctacaaaaat aaagttggtt
    21181 tactggataa ctcgcggatg aaaagcgata cccagtgggt gattgattcg atgcgggtaa
    21241 aaacgccggg tcatcggacg caaattggtt cgctctccgg tggtaatcag caaaaggtga
    21301 ttattggtcg ctggctacta acgcaaccag aaatattaat gctcgatgaa ccgacgcgcg
    21361 gtattgatgt cggggcgaag tttgaaattt atcagttaat tgccgaactg gcgaagaaag
    21421 gcaaggggat tattattatc tcctctgaaa tgcctgagtt gttagggata acagaccgta
    21481 ttctggtcat gagcaatggt ctcgtttccg gaattgtcga tacaaaaaca acaacgcaaa
    21541 acgaaattct gcgtcttgcg tctttgcacc tttaagatta ggggcttccc atgagtgcgt
    21601 taaataagaa aagttttctt acttacctga aagagggcgg tatttacgtc gttcttttag
    21661 ttttgctggc gattattatt ttccaggacc caacattttt aagtctgttg aacttaagta
    21721 atattctcac ccagtcatcg gtgcgtatta ttatcgcgct cggtgtggca gggttaattg
    21781 tcacccaggg gaccgatctt tctgctggtc gtcaggtagg gctggcggca gtggtggctg
    21841 cgacattatt gcagtccatg gataacgcca acaaagtgtt cccggaaatg gcgacgatgc
    21901 cgattgcgct ggttattctg attgtctgtg ccattggtgc ggtgatcggt ttgatcaacg
    21961 gtctgattat cgcttatctc aacgtgacgc cgttcattac cacgctcggc acgatgatca
    22021 tcgtctatgg aatcaactcg ctctattacg actttgtcgg ggcgtcgcca atttctggtt
    22081 ttgacagtgg cttctctacc tttgctcagg gctttgtcgt gctggggagt ttccgtctct
    22141 cttacatcac cttctacgcg ttgattgcgg tggcgttcgt ctgggtgttg tggaacaaaa
    22201 cccgcttcgg taagaacatt tttgccattg gcggtaaccc ggaagcggca aaagtatctg
    22261 gtgtcaacgt cggcctgaac ctgctgatga tctacgcgtt gtctggcgtg ttctatgcct
    22321 ttggcgggat gttagaagcc ggacgtatcg gctctgccac caacaacctc ggctttatgt
    22381 atgagctgga tgctatcgcg gcgtgcgtgg taggcggtgt atcgttcagc ggcggtgtgg
    22441 ggacggtgat tggcgtggtg accggggtaa ttatttttac cgtcatcaac tatggcctga
    22501 cgtatatcgg cgtaaaccca tactggcagt acatcatcaa aggggcgatt attatcttcg
    22561 ccgtagcgct ggattcactg aaatacgcgc gtaagaagtg atgtgattgt ggatattgcc
    22621 ggatgcgctg catccggcaa tcggtgccaa atgccggatg cgtctctggt tttacaacgt
    22681 taccgggtgt tctttctcgc ctttcttaaa cttcacttcc ccgagctcaa tacaacccac
    22741 cgggcagacg tgaccacaaa gcagacaacc cacacatttc tcggtattac aatgcggtgt
    22801 gcgggttttc tcgctccatt ccatcgcctg gtgaccgccg tcgtaacagg aaatataaca
    22861 gcgtccacag ccaacacatt tatcaagatt gatacgggga tagacaatat aactgcggtc
    22921 taaatcttcc gccgggacaa tattgttatt cgccagacct accatctcct gcagcgaatc
    22981 aaatccctga tcggcgagat aatgcgacaa cccgcttgcc atatcttcca ctatccgata
    23041 cccgtactgc atgatgccgg tggtcacctg taacgttgct gcgccgagca ataaaaactc
    23101 agccgcatcc tcccaggttt caatgccgcc gataccgctg attgggaaat cgcgcagttc
    23161 tggatgggtg cgcatctgct gaatgaagcg cagggcgatc ggttttaccg ctttaccgga
    23221 atatccggaa atactcgatt ttccgttaac gatcggcata ccgattttct gattaagatc
    23281 gatattggtg atggatttaa cggtgttaat cgccgcaatg ccatctgcgc cgccgcgctt
    23341 cgccgccagc gccacttcgc acatatcacc gatattcggc gtcatcttcg ccagcattgg
    23401 cagcgtggaa ccccgtttca ctgcccgaca atatttttct accagctccg ggctttgccc
    23461 gacatcgcta cccatcgcat gagaagtcat ttgcggacag gagaagttac actcgatcat
    23521 atccgcgcca gcttcttgca ccaggcgcgc cagctcctcc cattgctgct cattttcccc
    23581 catgatcgaa gcgatcaata ctttgtccgg gtaatcttcc ttcagctgac gcagggcggc
    23641 cagattctct tccaacggat gttcagcaat ctgctccata tttttgaagc cgataaaacc
    23701 ggtatcttct ttcaccagat gatcaaaacg cggcgagact tcgttggcga taaaaaagcc
    23761 gatcgtttta aacaccacgc cgccccaacc tgtgtcgtag gctttggcac acatctcata
    23821 gcagttgcct acaggcgaag aagagaggca gaacgggttg ggaaacttca cgccgcaaaa
    23881 agtaatcgaa agatctttcg ttaacatgag caagctccct ctaaatagtg atgaatcgcc
    23941 tcggcggctt ctttcccggt tttcacggca tagaccacgg ttttgtcacc ctcaacaata
    24001 tcgccagcag caaagacttg cggatcgcgg gtctgataat tttgtgtttt gatggtgtta
    24061 cgctgcggct ctaactccgc aaaggcatcc agtctggcat gctgaccgac ggcgagaata
    24121 attttatctg ccgccatcgt cagttcgccc ggtaaccgta catgcttaaa cgtgacttta
    24181 ttgccttcca cggctactgg cgtgaatcca tcgatgatcg aaacgcccag ctcccgggcg
    24241 ctggcaaact ctttttcgct tgccggaaac tcatctaact cttcacgcgc tacgcaggtt
    24301 accgcctgac agccgagaac tttcagcgtg ctggctacgt ccatcgcaac atcaccgccg
    24361 ccgataatta atgcgctttg tggaatgctg atatcgcctt gcgcctgtcg tgcacgttgc
    24421 aggaagtcga cggcaatctc aacgtcgcta tgctcaaaca gcgataaccc ggaaccgctc
    24481 gataacccca cggtgaccag taccgcgcgg ttttctgctt tcagctgctc aagggtgagt
    24541 gtgttaccga cttcgttgtt gcacttaatg gtcacgccca ttttttcaat acgggcgatc
    24601 tctgcatcca gcactgattg cggtaaccgg aattgcggaa taccgttacg caaccagcca
    24661 ccggggtgcg cttctttctc ataaatcgtg acgtcataac cctggtttgt cagtgttaca
    24721 ctggcctgca atccggcagg acctgcgcca ataatcgcga ctttgccgag cgttttagta
    24781 ccgggctgat aaatttccat cccggtttgt tgttcaaaat cagtaacaaa acgctgtaag
    24841 cgaccgatat caatgggagt atcaacaccg gcacgggtac aaccgctttg acataatttc
    24901 tccgtcgggc acactctggc acaaacggca ccgagggcat tattttcgcg aattgtctcg
    24961 gcagcgcctt taaaattacg aaagtagatt gagcgaataa atttccccgg atcggtctgt
    25021 gccgggcaag cctgactaca gggagcgtca tgacataata aacagcgaga ggcttcttta
    25081 atcgccagta aaggcgtaaa agccggagtg agttcatcca gataattttg ttgcggcatg
    25141 tgatcttcct taatgctatg agtaattaag gcgaacgcta aatatactcg tcatacttca
    25201 agttgca
//
LOCUS       DAZKHF020000042        25191 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2201S31241
            SAMN43267800-rid25106413.denovo.042, whole genome shotgun sequence.
ACCESSION   DAZKHF020000042 DAZKHF020000000
VERSION     DAZKHF020000042.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267800
            Sequence Read Archive: SRR30307263
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 25191)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25191)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 25191)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350581.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350581.2
            Genome Coverage       :: 151x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:21:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,652
            CDSs (total)                      :: 5,557
            Genes (coding)                    :: 5,320
            CDSs (with protein)               :: 5,320
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 237
            CDSs (without protein)            :: 237
            Pseudo Genes (ambiguous residues) :: 0 of 237
            Pseudo Genes (frameshifted)       :: 102 of 237
            Pseudo Genes (incomplete)         :: 130 of 237
            Pseudo Genes (internal stop)      :: 52 of 237
            Pseudo Genes (multiple problems)  :: 41 of 237
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25191
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267800-rid25106413.denovo.042"
                     /serotype="O 26:H11"
                     /isolate="2201S31241"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-01"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            <1..802
                     /locus_tag="ACHOSC_RS07025"
                     /old_locus_tag="pgaptmp_001405"
     CDS             <1..802
                     /locus_tag="ACHOSC_RS07025"
                     /old_locus_tag="pgaptmp_001405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001525780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HGU4547379.1"
                     /translation="IAITAYGMTYVLLLGDIDLSVGSTIALIGTFAALGASWGIPFIL
                     LVPLSIIAALALGMINGGLTAIAGIPSFIVTVATMGIFRGIAYIVTDGMPIMIKDDAF
                     LALGNGEFLYIPIPIWILIILLLINHFILTKTTFGRKIYITGGNKEAAIYSGINVTRL
                     KIKVFMITAVLAGISGMILASRRYSGQPNSALSYELDAIAAAVLGGTSLNGGYGTVVG
                     TVIGALTIGVINNGMNLMNVPYFYQMVVKGLVILVAVYFDVRNKRKRS"
     gene            824..1786
                     /locus_tag="ACHOSC_RS07030"
                     /old_locus_tag="pgaptmp_001406"
     CDS             824..1786
                     /locus_tag="ACHOSC_RS07030"
                     /old_locus_tag="pgaptmp_001406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000048618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0019200 - carbohydrate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar kinase"
                     /protein_id="HGU4547380.1"
                     /translation="MSKVFTIGEILVEIMASKIGQPFDQPGIWNGPYPSGAPAIFIDQ
                     VTRLGVPCGIISCVGNDGFGDINIHRLAADGVDIRGISVLPLEATGSAFVTYHNSGDR
                     DFIFNIKNAACGKLSAQHVDENILKDCTHFHIMGSSLFSFHMVDAVKKAVTIVKANGG
                     VISFDPNIRKEMLDIPEMRDALHFVLELTDIYMPSEGEVLLLSPHSTPERAIAGFLEE
                     GVKEVIVKRGNQGASYYSANEQFHVESYPVEEVDPTGAGDCFGGAWIACRQLGFDAHR
                     ALQYANACGALAVTRRGPMEGTSRLAEIEAFIQRHDMSIREAAQ"
     gene            1783..2829
                     /locus_tag="ACHOSC_RS07035"
                     /old_locus_tag="pgaptmp_001407"
     CDS             1783..2829
                     /locus_tag="ACHOSC_RS07035"
                     /old_locus_tag="pgaptmp_001407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000787050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldose 1-epimerase family protein"
                     /protein_id="HGU4547381.1"
                     /translation="MKNEIWSWIGDLSQVAGIKHYELRSGRAKGTEAFDVRTGAGLAF
                     TVVKDRALDIAWASYKDTALSFITPNGIVAPAFFESQGNGFLRSFYAGLLTTCGLSYI
                     GTPCEDEGETLGLHGRLAATPAEEVGYRTERTDDGIEFVINGKVRETRLFGENLTLER
                     TIRCRYGENVLRIEDKVTNHGFTRQPLQILYHFNYGWPLLSPQAEILLSAKSVTPRTP
                     HAAEGLTSHLEICTPQPGFDEQVYYLTLNSDSQGMSKVALVNAELGWGIYEKFDTMQL
                     PNFIQWKNLGAGEYVMGLEVSNSFPDGRDKERAQGRLPFIEPGETKKYCFELGIVDGD
                     AEMSALKAEIAGYR"
     gene            complement(2921..4270)
                     /gene="accC"
                     /locus_tag="ACHOSC_RS07040"
                     /old_locus_tag="pgaptmp_001408"
     CDS             complement(2921..4270)
                     /gene="accC"
                     /locus_tag="ACHOSC_RS07040"
                     /old_locus_tag="pgaptmp_001408"
                     /EC_number="6.3.4.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009483995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0004075 - biotin carboxylase activity
                     [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxylase
                     subunit"
                     /protein_id="HGU4547382.1"
                     /translation="MLDKIVIANRGEIALRILRACKELGIKTVAVHSSADRDLKHVLL
                     ADETVCIGPAPSVKSYLNIPAIISAAEITGAVAIHPGYGFLSENANFAEQVERSGFIF
                     IGPKAETIRLMGDKVSAIAAMKKAGVPCVPGSDGPLGDDMDKNRAIAKRIGYPVIIKA
                     SGGGGGRGMRVVRGDAELAQSISMTRAEAKAAFSNDMVYMEKYLENPRHVEIQVLADG
                     QGNAIYLAERDCSMQRRHQKVVEEAPAPGITPELRRYIGERCAKACVDIGYRGAGTFE
                     FLFENGEFYFIEMNTRIQVEHPVTEMITGVDLIKEQLRIAAGQPLSIKQEEVHVRGHA
                     VECRINAEDPNTFLPSPGKITRFHAPGGFGVRWESHIYAGYTVPPYYDSMIGKLICYG
                     ENRDVAIARMKNALQELIIDGIKTNVDLQIRIMNDENFQHGGTNIHYLEKKLGLQEK"
     gene            complement(4281..4751)
                     /gene="accB"
                     /locus_tag="ACHOSC_RS07045"
                     /old_locus_tag="pgaptmp_001409"
     CDS             complement(4281..4751)
                     /gene="accB"
                     /locus_tag="ACHOSC_RS07045"
                     /old_locus_tag="pgaptmp_001409"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxyl carrier
                     protein"
                     /protein_id="HGU4547383.1"
                     /translation="MDIRKIKKLIELVEESGISELEISEGEESVRISRAAPAASFPVM
                     QQAYAAPIMQQPAQSNAAAPATVPSMEAPAAAEISGHIVRSPMVGTFYRTPSPDAKAF
                     IEVGQKVNVGDTLCIVEAMKMMNQIEADKSGTVKAILVESGQPVEFDEPLVVIE"
     gene            complement(5617..6447)
                     /locus_tag="ACHOSC_RS07050"
                     /old_locus_tag="pgaptmp_001410"
     CDS             complement(5617..6447)
                     /locus_tag="ACHOSC_RS07050"
                     /old_locus_tag="pgaptmp_001410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000838295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4547384.1"
                     /translation="MTSLFNRLTGKAVSRTAFVEHLGQEVVQHHPNWKVMISTDHKLM
                     RIDTGHQTVEITLDDAYKRYQAAPDPLQEVAADMLKQLAEMLPTGRCIVPVIKEKAWL
                     DTVRQQLSQYESDPEVLNASLDFAWEDFNDELIILFAEDGSETMRYLMRYEVTIDREQ
                     AISNLKQILPEVTAEIVDTGANDGKVAVISAGGTYEPSLMLIDDLWVQSPDPECGDLL
                     VTVPSRGKLFFTHNDQPANLSALRQLTKQCFREDDHPLTTQIFIRRNGHFYPWVETLH
                     "
     gene            complement(6484..7458)
                     /locus_tag="ACHOSC_RS07055"
                     /old_locus_tag="pgaptmp_001411"
     CDS             complement(6484..7458)
                     /locus_tag="ACHOSC_RS07055"
                     /old_locus_tag="pgaptmp_001411"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004024 - alcohol dehydrogenase (NAD+)
                     activity, zinc-dependent [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0030554 - adenyl nucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MDR family oxidoreductase"
                     /protein_id="HGU4547385.1"
                     /translation="MQALLLEQQDGKTLASVQTLDESRLPEDDVTVDVHWSSLNYKDA
                     LAITGKGKIIRNFPMIPGIDFAGTVRTSEDPRFHAGQEVLLTGWGVGENHWGGLAEQA
                     RVKGDWLVAMPQGLDARKAMIIGTAGFTAMLCVMALEDAGVRPQDGEIVVTGASGGVG
                     STAVALLHKLGYQVVAVSGRESTHEYLKSLGASRILPRDEFAESRPLEKQVWAGAIDT
                     VGDKVLAKVLAQMNYGGCVAACGLAGGFTLPTTVMPFILRNVRLQGVDSVMTPPERRA
                     QAWQRLVADLPESFYTQAAKEISLSEAPNFAEAIINNQIQGRTLVKVN"
     gene            7610..9550
                     /gene="csrD"
                     /locus_tag="ACHOSC_RS07060"
                     /old_locus_tag="pgaptmp_001412"
     CDS             7610..9550
                     /gene="csrD"
                     /locus_tag="ACHOSC_RS07060"
                     /old_locus_tag="pgaptmp_001412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNase E specificity factor CsrD"
                     /protein_id="HGU4547386.1"
                     /translation="MRLTTKFSAFVTLLTGLTIFVTLLGCSLSFYNAIQYKFSHRVQA
                     VATAIDTHLVSNDFSTLRPQITELMMSADIVRVDLLHGDKQVYTLARNGSYRPVGSSD
                     LFRELSVPLIKHPGMSLRLVYQDPMGNYFHSLMTTAPLTGAIGFIIVMLFLAVRWLQR
                     QLAGQELLETRATRILNGERGSNVLGTIYEWPPRTSSALDTLLREIQNAREQHSRLDT
                     LIRSYAAQDVKTGLNNRLFFDNQLATLLEDQEKVGTHGIVMMIRLPDFNMLSDTWGHS
                     QVEEQFFTLTNLLSTFMMRYPGALLARYHRSDFAALLPHRTLKEAESIAGQLIKAVDT
                     LPNNKMLDRDDMIHIGICAWRSGQDTEQVMEHAESATRNAGLQGGNSWAIYDDSLPEK
                     GRGNVRWRTLIEQMLSRGGPRLYQKPAVTREGQVHHRELMCRIFDGNEEVSSAEYMPM
                     VLQFGLSEEYDRLQISRLIPLLRYWPEENLAIQVTVESLIRPRFQRWLRDTLMQCEKS
                     QRKRIIIELAEADVGQHISRLQPVIRLVNALGVRVAVNQAGLTLVSTSWIKELNVELL
                     KLHPGLVRNIEKRTENQLLVQSLVEACSGTSTQVYATGVRSRSEWQTLIQRGVTGGQG
                     DFFASSQPLDTNVKKYSQRYSV"
     gene            9855..10898
                     /gene="mreB"
                     /locus_tag="ACHOSC_RS07065"
                     /old_locus_tag="pgaptmp_001413"
     CDS             9855..10898
                     /gene="mreB"
                     /locus_tag="ACHOSC_RS07065"
                     /old_locus_tag="pgaptmp_001413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000913396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreB"
                     /protein_id="HGU4547387.1"
                     /translation="MLKKFRGMFSNDLSIDLGTANTLIYVKGQGIVLNEPSVVAIRQD
                     RAGSPKSVAAVGHDAKQMLGRTPGNIAAIRPMKDGVIADFFVTEKMLQHFIKQVHSNS
                     FMRPSPRVLVCVPVGATQVERRAIRESAQGAGAREVFLIEEPMAAAIGAGLPVSEATG
                     SMVVDIGGGTTEVAVISLNGVVYSSSVRIGGDRFDEAIINYVRRNYGSLIGEATAERI
                     KHEIGSAYPGDEVREIEVRGRNLAEGVPRGFTLNSNEILEALQEPLTGIVSAVMVALE
                     QCPPELASDISERGMVLTGGGALLRNLDRLLMEETGIPVVVAEDPLTCVARGGGKALE
                     MIDMHGGDLFSEE"
     gene            10964..12067
                     /gene="mreC"
                     /locus_tag="ACHOSC_RS07070"
                     /old_locus_tag="pgaptmp_001414"
     CDS             10964..12067
                     /gene="mreC"
                     /locus_tag="ACHOSC_RS07070"
                     /old_locus_tag="pgaptmp_001414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreC"
                     /protein_id="HGU4547388.1"
                     /translation="MKPIFSRGPSLQIRLILAVLVALGIIIADSRLGTFSQIRTYMDT
                     AVSPFYFVSNAPRELLDGVSQTLASRDQLELENRALRQELLLKNSELLMLGQYKQENA
                     RLRELLGSPLRQDEQKMVTQVISTVNDPYSDQVVIDKGSVNGVYEGQPVISDKGVVGQ
                     VVAVAKLTSRVLLICDATHALPIQVLRNDIRVIAAGNGCTDDLQLEHLPANTDIRVGD
                     VLVTSGLGGRFPEGYPVAVVSSVKLDTQRAYTVIQARPTAGLQRLRYLLLLWGADRNG
                     ANPMTPEEVHRVANERLMQMMPQVLPSPDAMGPKLPEPATGIAQPTPQQPTTGNAATA
                     PAAPTQPAANRSPQRATPPQSGAQPPARAPGGQ"
     gene            12067..12555
                     /gene="mreD"
                     /locus_tag="ACHOSC_RS07075"
                     /old_locus_tag="pgaptmp_001415"
     CDS             12067..12555
                     /gene="mreD"
                     /locus_tag="ACHOSC_RS07075"
                     /old_locus_tag="pgaptmp_001415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001487551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreD"
                     /protein_id="HGU4547389.1"
                     /translation="MASYRSQGRWVIWLSFLIALLLQIMPWPDNLIVFRPNWVLLILL
                     YWILALPHRVNVGTGFVMGAILDLISGSTLGVRVLAMSIIAYLVALKYQLFRNLALWQ
                     QALVVMLLSLVVDIIVFWAEFLVINVSFRPEVFWSSVVNGVLWPWIFLLMRKVRQQFA
                     VQ"
     gene            12564..13157
                     /gene="yhdE"
                     /locus_tag="ACHOSC_RS07080"
                     /old_locus_tag="pgaptmp_001416"
     CDS             12564..13157
                     /gene="yhdE"
                     /locus_tag="ACHOSC_RS07080"
                     /old_locus_tag="pgaptmp_001416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside triphosphate pyrophosphatase YhdE"
                     /protein_id="HGU4547390.1"
                     /translation="MTSLYLASGSPRRQELLAQLGVTFERIVTGIEEQRQPQESAQQY
                     VVRLAREKAQAGVAQTAQDLPVLGADTIVILNGEVLEKPRDAEHAAQMLRKLSGQTHQ
                     VMTAVALADSQHILDCLVVTDVTFRTLTDEDIAGYVASGEPLDKAGAYGIQGLGGCFV
                     RKINGSYHAVVGLPLVETYELLSNFNALREKRDKHDG"
     gene            13147..14616
                     /gene="rng"
                     /locus_tag="ACHOSC_RS07085"
                     /old_locus_tag="pgaptmp_001417"
     CDS             13147..14616
                     /gene="rng"
                     /locus_tag="ACHOSC_RS07085"
                     /old_locus_tag="pgaptmp_001417"
                     /EC_number="3.1.26.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006818051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004540 - RNA nuclease activity [Evidence
                     IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease G"
                     /protein_id="HGU4547391.1"
                     /translation="MTAELLVNVTPSETRVAYIDGGILQEIHIEREARRGIVGNIYKG
                     RVSRVLPGMQAAFVDIGLDKAAFLHASDIMPHTECVAGEEQKQFTVRDISELVRQGQD
                     LMVQVVKDPLGTKGARLTTDITLPSRYLVFMPGASHVGVSQRIESESERERLKKVVAE
                     YCDEQGGFIIRTAAEGVGEAELASDAAYLKRVWTKVMERKKRPQTRYQLYGELALAQR
                     VLRDFADAELDRIRVDSRLTYEALLEFTSEYIPEMTSKLEHYTGRQPIFDLFDVENEI
                     QRALERKVELKSGGYLIIDQTEAMTTVDINTGAFVGHRNLDDTIFNTNIEATQAIARQ
                     LRLRNLGGIIIIDFIDMNNEDHRRRVLHSLEQALSKDRVKTSVNGFSALGLVEMTRKR
                     TRESIEHVLCNECPTCHGRGTVKTVETVCYEIMREIVRVHHAYDSDRFLVYASPAVAE
                     ALKGEESHSLAEVEIFVGKQVKVQIEPLYNQEQFDVVMM"
     gene            14684..18484
                     /gene="yhdP"
                     /locus_tag="ACHOSC_RS07090"
                     /old_locus_tag="pgaptmp_001418"
     CDS             14684..18484
                     /gene="yhdP"
                     /locus_tag="ACHOSC_RS07090"
                     /old_locus_tag="pgaptmp_001418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709041.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AsmA2 domain-containing protein YhdP"
                     /protein_id="HGU4547392.1"
                     /translation="MRRLPGILLLTGAALVVIAALLVSGLRIALPHLDAWRPEILNKI
                     ESATGMPVEASQLSASWQNFGPTLEAHDIRAELKDGGEFSVKRVTLALDVWQSLLHMR
                     WQFRDLTFWQLRFRTNTPITSGGSDDSLEASHISDLFLRQFDHFDLRDSEVSFLTPSG
                     QRAELAIPQLTWLNDPRRHRAEGLVSLSSLTGQHGVMQVRMDLRDDEGLLSNGRVWLQ
                     ADDIDLKPWLGKWMQDNIALETAQFSLEGWMTIDKGDVTGGDVWLKQGGASWLGEKQT
                     HTLSVDNLTAHITRENPGWQFSIPDTRITMDGKPWPSGALTLAWIPEQDVGGKDNKRS
                     DELRIRASNLELAGLEGIRPLAAKLSPALGDVWRSTQPSGKINTLALDIPLQAADKTR
                     FQASWSDLAWKQWKLLPGAEHFSGTLSGSVENGLLTASMKQAKMPYETVFRAPLEIAD
                     GQATISWLNNDKGFQLDGRNIDVKAKAVHARGGFRYLQPANDEPWLGILAGISTDDGS
                     QAWRYFPENLMGKDLVDYLSGAIQGGEADNATLVYGGNPQLFPYKHNEGQFEVLVPLR
                     NAKFAFQPDWPALTNLDIELDFINDGLWMKTDGVNLGGVRASNLTAVIPDYSKEKLLI
                     DADIKGPGKAVGPYFDETPLKDSLGATLQELQLDGDVNARLHLDIPLNGELVTAKGEV
                     TLRNNSLFIKPLDSTLKNLSGKFSFINGDLQSEPLTASWFNQPLNVDFSTKEGAKAYQ
                     VAVNLNGNWQPAKTSVLPEAVNEALSGSVAWDGKVGIDLPYHAGATYNVELNGDLNNV
                     SSHLPSPLAKPAGEPLAVNVKVDGNLNSFELTGQAGADNHFNSRWLLGQKLTLDRAIW
                     AADSKTLPPLPEQSGVELNMPPMNGAEWLALFQKGAAESVGGAASFPQHITLRTPMLS
                     LGNQQWNNLSIVSQPTANGTLVEAQGREINATLAMRNNAPWLANIKYLYYNPSVAKTR
                     GDSTPSSPFPTTERINFRGWPDAQIRCTECWFWGQKFGRIDSDITISGDTLTLTNGLI
                     DTGFSRLTADGEWVNNPENERTSLKGKLRGQKIDAAAEFFGVTTPIRQSSFNVDYDLH
                     WRKAPWQPDEATLNGIIHTQLGKGEITEINTGHAGQLLRLLSVDALMRKLRFDFRDTF
                     GEGFYFDSIRSTAWIKDGVMHTDDTLVDGLEADIAMKGSVNLVRRDLNMEAVVAPEIS
                     ATVGVAAAFAVNPIVGAAVFAASKVLGPLWSKVSILRYHISGPLDDPQINEVLRQPRK
                     EKAQ"
     gene            18640..20085
                     /gene="tldD"
                     /locus_tag="ACHOSC_RS07095"
                     /old_locus_tag="pgaptmp_001419"
     CDS             18640..20085
                     /gene="tldD"
                     /locus_tag="ACHOSC_RS07095"
                     /old_locus_tag="pgaptmp_001419"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloprotease TldD"
                     /protein_id="HGU4547393.1"
                     /translation="MSLNLVSEQLLAANGLKHQDLFAILGQLAERRLDYGDLYFQSSY
                     HESWVLEDRIIKDGSYNIDQGVGVRAISGEKTGFAYADQISLLALEQSAQAARTIVRD
                     SGDGKVQTLGAVEHSPLYTSVDPLQSMSREEKLDILRRVDKVAREADKRVQEVTASLS
                     GVYELILVAATDGTLAADVRPLVRLSVSVLVEEDGKRERGASGGGGRFGYEFFLADLD
                     GEVRADAWAKEAVRMALVNLSAVAAPAGTMPVVLGAGWPGVLLHEAVGHGLEGDFNCR
                     GTSVFSGQVGELVASELCTVVDDGTMVDRRGSVAIDDEGTPGQYNVLIENGILKGYMQ
                     DKLNARLMGMTPTGNGRRESYAHLPMPRMTNTYMLPGKSTPQEIIESVEYGIYAPNFG
                     GGQVDITSGKFVFSTSEAYLIENGKVTKPVKGATLIGSGIETMQQISMVGNDLKLDNG
                     VGVCGKEGQSLPVGVGQPTLKVDNLTVGGTA"
     gene            complement(20213..21142)
                     /gene="aaeR"
                     /locus_tag="ACHOSC_RS07100"
                     /old_locus_tag="pgaptmp_001420"
     CDS             complement(20213..21142)
                     /gene="aaeR"
                     /locus_tag="ACHOSC_RS07100"
                     /old_locus_tag="pgaptmp_001420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional activator AaeR"
                     /protein_id="HGU4547394.1"
                     /translation="MERLKRMSVFAKVVEFGSFTAAARQLQMSVSSISQTVSKLEDEL
                     QVKLLNRSTRSIGLTEAGRIYYQGCRRMLHEVQDVHEQLYAFNNTPIGTLRIGCSSTM
                     AQNVLAGLTAKMLKEYPGLSVNLVTGIPAPDLIADGLDVVIRVGALQDSSLFSRRLGA
                     MPMVVCAAKSYLTQYGIPEKPADLSSHSWLEYSVRPDNEFELIAPEGISTRLIPQGRF
                     VTNDPMTLVRWLTAGAGIAYVPLMWVINEINRGELEILLPRYQSDPRPVYALYTEKDK
                     LPLKVQVVINSLTDYFVEVGKLFQEMHGRGKEK"
     gene            21325..21528
                     /gene="aaeX"
                     /locus_tag="ACHOSC_RS07105"
                     /old_locus_tag="pgaptmp_001421"
     CDS             21325..21528
                     /gene="aaeX"
                     /locus_tag="ACHOSC_RS07105"
                     /old_locus_tag="pgaptmp_001421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000051842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="p-hydroxybenzoic acid efflux pump operon protein
                     AaeX"
                     /protein_id="HGU4547395.1"
                     /translation="MSLFPVIVVFGLSFPPIFFELLLSLAIFWLVRRVLVPTGIYDFV
                     WHPALFNTALYCCLFYLISRLFV"
     gene            21536..22468
                     /gene="aaeA"
                     /locus_tag="ACHOSC_RS07110"
                     /old_locus_tag="pgaptmp_001422"
     CDS             21536..22468
                     /gene="aaeA"
                     /locus_tag="ACHOSC_RS07110"
                     /old_locus_tag="pgaptmp_001422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="p-hydroxybenzoic acid efflux pump subunit AaeA"
                     /protein_id="HGU4547396.1"
                     /translation="MKTLIRKFSRTAITVVLVILAFIAIFNAWVYYTESPWTRDARFS
                     ADVVAIAPDVSGLITQVNVHDNQLVKKGQILFTIDQPRYQKALEEAQADVAYYQVLAQ
                     EKRQEAGRRNRLGVQAMSREEIDQANNVLQTVLHQLAKAQATRDLAKLDLERTVIRAP
                     ADGWVTNLNVYTGEFITRGSTAVALVKQNSFYVLAYMEETKLEGVRPGYRAEITPLGS
                     NKVLKGTVDSVAAGVTNASSTRDDKGMATIDSNLEWVRLAQRVPVRIRLDNQQENIWP
                     AGTTATVVVTGKQDRDESQDSFFRKMAHRLREFG"
     gene            22474..24441
                     /gene="aaeB"
                     /locus_tag="ACHOSC_RS07115"
                     /old_locus_tag="pgaptmp_001423"
     CDS             22474..24441
                     /gene="aaeB"
                     /locus_tag="ACHOSC_RS07115"
                     /old_locus_tag="pgaptmp_001423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="p-hydroxybenzoic acid efflux pump subunit AaeB"
                     /protein_id="HGU4547397.1"
                     /translation="MGIFSIANQHIRFAVKLATAIVLALFVGFHFQLETPRWAVLTAA
                     IVAAGPAFAAGGEPYSGAIRYRGFLRIIGTFIGCIAGLVIIIAMIRAPLLMILVCCIW
                     AGFCTWISSLVRIENSYAWGLAGYTALIIVITIQPEPLLTPQFAVERCSEIVIGIVCA
                     IMADLLFSPRSIKQEVDRELESLLVAQYQLMQLCIKHGDGEVVDKAWGDLVRRTTALQ
                     SMRSNLNMESSRWARANRRLKAINTLSLTLITQSCETYLIQNTRPELITDTFREFFDT
                     PVETAQDVHKQLKRLRRVIAWTGERETPVTIYSWVAAATRYQLLKRGVISNTKINATE
                     EEILQGEPEVKVESAERHHAMVNFWRTTLSCILGTLFWLWTGWTSGSGAMVMIAVVTS
                     LAMRLPNPRMVAIDFIYGTLAALPLGLLYFLVIIPNTQQSMLLLCISLAVLGFFLGIE
                     VQKRRLGSMGALASTINIIVLDNPMTFHFSQFLDSALGQIVGCVLAFTVILLVRDKSR
                     DRTGRVLLNQFVSAAVSAMTTNVARRKENHLPALYQQLFLLMNKFPGDLPKFRLALTM
                     IIAHQRLRDAPIPVNEDLSAFHRQMRRTADHVISARSDDKRRRYFGQLLEELEIYQEK
                     LRIWQAPPQVTEPVHRLAGMLHKYQHALTDS"
     gene            24533..24805
                     /locus_tag="ACHOSC_RS07120"
                     /old_locus_tag="pgaptmp_001424"
     CDS             24533..24805
                     /locus_tag="ACHOSC_RS07120"
                     /old_locus_tag="pgaptmp_001424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="barstar family protein"
                     /protein_id="HGU4547398.1"
                     /translation="MNIYTFDFDEIESQEDFYRDFSQTFGLAKDKVRDLDSLWDVLMN
                     DVLPLPLEIEFVHLGEKTRRRFGALILLFDEAEEELEGHLRFNVRH"
     gene            complement(24861..25124)
                     /gene="yhcN"
                     /locus_tag="ACHOSC_RS07125"
                     /old_locus_tag="pgaptmp_001425"
     CDS             complement(24861..25124)
                     /gene="yhcN"
                     /locus_tag="ACHOSC_RS07125"
                     /old_locus_tag="pgaptmp_001425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417705.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxide/acid stress response protein YhcN"
                     /protein_id="HGU4547399.1"
                     /translation="MKIKTTVAALSVLSVLSFGAFAADSIDAAQAQNREAIGTVSVSG
                     VASSPMDMREMLNKKAEEKGATAYQITEARSGDTWHATAELYK"
BASE COUNT         5933 a         6142 c         6853 g         6263 t
ORIGIN      
        1 aattgcaatt accgcctatg gaatgactta tgtacttttg cttggcgata ttgatttatc
       61 ggtcggttca accattgcat tgattggcac atttgctgcg cttggggctt catggggaat
      121 tccatttatt ttattggtcc cattatccat tattgcagca ctggctctgg ggatgataaa
      181 tggtggcttg acggctatag cggggatccc gtcgtttatt gtcactgttg ctactatggg
      241 gatcttccgt ggtatcgctt atatagtgac cgatggtatg cccatcatga ttaaagatga
      301 tgcctttctg gcactgggga atggagaatt tctctatata cctattccaa tctggatatt
      361 aatcatctta ttgctgatta accacttcat tcttactaaa acaacctttg gacgaaaaat
      421 ttatatcact ggtggtaata aagaagcggc aatttattca gggataaatg tcacccgctt
      481 gaaaattaaa gtctttatga ttacagccgt gctggcaggt atcagcggga tgatcctcgc
      541 ctcacgccgc tattccgggc aaccgaattc cgcgttgagc tacgaactgg atgctattgc
      601 tgcggctgta ctgggaggca ccagcctgaa cggcggctat ggaaccgtgg tggggacggt
      661 gatcggcgca ttaactatcg gcgttattaa taatggcatg aacttaatga atgttcccta
      721 tttttaccag atggtggtta aaggattagt cattcttgta gccgtttatt ttgacgtaag
      781 aaataaaaga aaacgcagct aatcatttca aggaattaat catatgagta aggtcttcac
      841 aattggagaa attctggttg aaattatggc gagtaaaatc ggccagccat ttgatcaacc
      901 aggtatctgg aacggccctt atcccagtgg tgcgcccgct atttttatcg accaggtgac
      961 acgtcttggc gttccgtgcg gcatcattag ctgtgtgggg aatgatggct ttggtgatat
     1021 taatatacat cgtctggctg ctgacggtgt ggatatcagg gggatttcag tcttacctct
     1081 ggaagctacc gggagtgcct ttgttactta tcataactct ggcgatcgcg attttatttt
     1141 taatattaag aatgccgctt gcggtaaatt atcggcgcag catgttgatg aaaatattct
     1201 gaaggactgt acccattttc atattatggg ctcttccctg ttttcgtttc atatggtaga
     1261 cgcagtaaaa aaagcggtaa ctatcgttaa agcaaatggt ggtgttattt ctttcgaccc
     1321 taatattcgt aaagagatgc tcgatattcc agaaatgcgt gacgccctcc actttgttct
     1381 tgagttaacg gatatctata tgcccagcga gggcgaagtg ttgctcttgt cgccacattc
     1441 gacgccggag cgtgcaatcg caggttttct tgaagaaggt gttaaagagg tgattgtgaa
     1501 gcgcggcaat caaggtgcca gttactactc cgcgaatgag cagtttcatg ttgagagcta
     1561 tccggtggaa gaagttgacc cgaccggtgc gggagactgc tttggtggcg cgtggattgc
     1621 ctgccgccag ctagggtttg acgcccatcg agcgctgcaa tatgccaatg cctgcggtgc
     1681 gctggcggta accagacgtg gcccaatgga gggaacgtct cgccttgcgg aaatcgaagc
     1741 gttcatccag cgccatgaca tgtccattcg ggaagcagca caatgaaaaa cgaaatttgg
     1801 tcgtggattg gcgatctctc gcaggttgcc ggaattaaac actatgagtt acgtagtggc
     1861 agagcaaagg gtacagaagc gtttgatgtc cgtactggtg cgggactggc atttacggtg
     1921 gtaaaagatc gcgcgctgga tattgcatgg gccagctaca aggacacggc gctttctttt
     1981 atcaccccca acggcattgt cgcccccgct ttttttgagt ctcagggcaa cggattttta
     2041 cgtagctttt acgcggggtt actgactacc tgcggactta gctacattgg cacgccttgt
     2101 gaggatgaag gagaaacgct ggggttacac gggcgtttgg ctgcgactcc agcagaagag
     2161 gtggggtatc gcacagaacg gaccgacgac ggcattgaat ttgtcatcaa tggcaaagta
     2221 cgggaaacgc gactgttcgg cgaaaatctg acgctggaaa ggaccattcg ctgccgttat
     2281 ggcgaaaatg tgctgcgcat tgaagacaag gtgactaatc atggtttcac ccgccagccg
     2341 ctgcaaattc tttatcactt taattacggc tggcctttat tatcgccaca ggcagaaatc
     2401 ttgctgtcgg ctaaaagcgt aacgccacga actccgcatg cggcggaagg gttaaccagt
     2461 catctggaga tttgtacccc acagcccggt tttgacgagc aggtttacta tctcacgttg
     2521 aatagcgaca gtcaggggat gagtaaagtt gctctggtga acgccgaact ggggtgggga
     2581 atttacgaga aattcgacac catgcagcta cccaacttta tccagtggaa aaatttaggc
     2641 gcgggtgagt atgttatggg actggaggtc agtaatagtt ttcctgacgg tcgtgataaa
     2701 gaacgagcgc aggggcgtct gccctttatt gagccagggg aaacaaagaa atactgcttt
     2761 gagctgggaa ttgttgatgg cgatgctgaa atgagcgcgt tgaaagcgga aattgcaggc
     2821 taccgttagc taacggcaat aaaaacgccg ggcagcgtta acaggcccgg cgttgattac
     2881 gttctttgct aagcgcgatg aaaacaccgc gcgtcacaaa ttatttttcc tgaagaccga
     2941 gttttttctc cagatagtgg atgttagtgc caccatgctg gaagttctcg tcattcatga
     3001 tgcggatctg cagatcaacg ttggttttga taccgtcgat gatcagctcc tgcagcgcat
     3061 tcttcatgcg ggcaatcgcc acgtcacggt tttcaccgta gcaaatcagc ttaccgatca
     3121 ttgagtcata gtacggcggt acggtgtagc ccgcgtagat atgagactcc caacgtacgc
     3181 caaaaccgcc aggtgcgtgg aaacgggtga ttttgcctgg gcttggcagg aaggtgttcg
     3241 gatcttcggc gttgatacga cattccaccg catggccgcg aacgtgaact tcttcttgct
     3301 tgatcgacag cggttgaccg gcagcgatac gcagctgttc tttgatcagg tcaacgccgg
     3361 tgatcatttc tgtaaccggg tgttctacct gaatacgggt gttcatttcg atgaaataga
     3421 actcgccgtt ttcgaacagg aactcgaaag tacctgcacc gcgatagccg atatcaacac
     3481 acgctttagc gcaacgttcg ccgatgtagc gacgcagttc cggggtaatg cccggtgctg
     3541 gtgcttcttc gaccactttc tggtggcggc gctgcatgga gcagtcacgt tccgccagat
     3601 agatagcgtt gccctgaccg tcagccagta cctgaatctc gacgtggcga ggattttcca
     3661 ggtatttctc catgtaaacc atatcgttgc tgaaagcagc tttcgcttcc gcacgggtca
     3721 tggagatgga ttgtgccagt tcagcgtcgc cgcgcactac gcgcataccg cgaccaccgc
     3781 cgccgccgga ggctttgata atcaccggat aaccaatgcg tttagcaatg gcacggtttt
     3841 tatccatatc gtcgcccaac gggccgtcag aacccggtac gcaagggacg cccgcttttt
     3901 tcatcgccgc gattgcggat actttgtcgc ccatcaggcg aatggtttct gctttcgggc
     3961 cgatgaagat aaagccggag cgttcaacct gctcggcaaa gttggcgttc tcggagagga
     4021 agccgtaacc cggatggatt gctactgcgc cggtgatttc agcggcgctg atgattgccg
     4081 ggatgttcag ataacttttt actgacggag cagggccaat acagaccgtt tcatctgcca
     4141 gtaatacgtg ttttagatcg cgatccgcgc tggagtgcac agcgacagtc ttgatgccca
     4201 gttctttaca ggcacgaaga atacgcaatg caatctcgcc gcggttggca ataacaattt
     4261 tatccagcat gttcgcctcg ttactcgatg acgaccagcg gctcgtcaaa ttctaccggt
     4321 tgtccacttt cgaccagaat tgctttcacg gtaccggatt tgtccgcttc gatctggttc
     4381 atcattttca tggcttcaac gatgcacagg gtatcgccca cgttgacttt ctgacccact
     4441 tcgatgaacg cttttgcgtc cgggcttggg gtgcggtaga aagtaccaac catcggggaa
     4501 cgtacgatgt gaccactgat ttccgctgct gctggcgctt ccatggaagg aacggtcgcc
     4561 ggagcggctg cgttagattg agctggctgc tgcattattg gtgcagcgta agcttgttgc
     4621 atcacaggga aacttgcggc aggagctgca cggctaatgc gtactgactc ttcgccttca
     4681 gaaatttcca gttcggagat gcctgattct tcaaccagct cgatcagttt tttaatctta
     4741 cgaatatcca tgagtgggtt ccgtactctt tgtttagtgt gattgtgaca ggcgtttcac
     4801 cgccgtctgt aaagcgtatg ccccgacagc tgtatgcata gcgataaatt ccagcaggcc
     4861 gggacgccgg tctattttgc cccagagtgt caatgttaga cttgacggac attgtgcagc
     4921 gatctgctgc tatcctccgg caaaaaacaa aatatacctt cgttgcgcca atgtcacctt
     4981 ttccgcagcg caaaaactgc gtcagggagg acgaggtcgc acattataac gatttcgtag
     5041 caattggcag ctaaatactg gtcttatcag ggaagataat caacagctaa catgtaaata
     5101 accttcaaca ccgtgtaatt tgcaacaagc cgcacaattc acgaaattag cgccatcatc
     5161 gacggaactt cttataacgt aaggtgaaaa gctgtaaagc cagccctgcg aagatgaggg
     5221 gctgcaatga gataatcttc acagaccaca aataatgtag ggatgccagg atcgcgacaa
     5281 gatagacgac attacgcagc ggttgctggg gattacccaa ttttcactgc attgtctgga
     5341 tagatgttaa tgctaaagca cgcaaaatta accgattgac aatgctcagc attaaatagg
     5401 ttcgagtgat taatttgtag tcaaacaaaa tcagattatt ccccccattt tgcagaaggt
     5461 aaactgtttt gtcaggcggc agtgcggcta tctgatgctt tcagaatttg ccacctgaca
     5521 ataaaaaagc cgatttggta gcaaaatcgg cttcggtgag tttgcagttt tcatcacata
     5581 ctccgacaag ctttttcaag catgacggag gttgcgttaa tgcaaggttt ctacccaggg
     5641 ataaaagtga ccgttacggc ggatgaatat ctgtgttgtc agaggatggt catcctcacg
     5701 gaagcattgt tttgttagct gtcgtaatgc actgagattt gctggctgat cattgtgggt
     5761 gaaaaacagt tttcctctgg agggaacggt cactaacagg tcgccgcatt caggatcggg
     5821 tgattgcacc caaagatcgt caatgagcat cagactgggc tcataagtac cccctgcgga
     5881 gataacggca acttttccat cgtttgcgcc tgtatcaacg atttcagccg taacttccgg
     5941 caatatttgc ttcaaattac tgatggcttg ttcgcggtca atggtcactt cgtagcgcat
     6001 cagatagcgc atggtttcgc taccatcttc agcaaacaag ataattaact catcgttaaa
     6061 gtcttcccag gcaaaatcca gcgaggcgtt gaggacctct ggatcagact catactggct
     6121 aagctgttgg cgaacggtat ccagccaggc tttttcttta atcaccggaa caatacagcg
     6181 cccggtcggc agcatttctg caagctgttt cagcatatct gcagccactt cttgcagcgg
     6241 gtctggtgcg gcttgataac gtttgtaggc atcatcaagg gtgatttcca ccgtttggtg
     6301 acccgtatca atgcgcatca atttgtggtc agtcgaaatc atgactttcc agtttggatg
     6361 atgttgtaca acttcctgac caaggtgttc gacgaaagct gtccggctaa ccgcttttcc
     6421 cgttaaccga ttgaataatg atgtcaaaat gagttccctc tcttattatt ccttgctaaa
     6481 tggttagtta accttcacca gcgtgcgacc ctggatctgg ttattaatga tggcctcggc
     6541 aaagttcggt gcctctgaca gagatatctc ttttgccgcc tgggtataga atgattccgg
     6601 taaatcggcg accagtcgct gccaggcttg tgcgcggcgt tctggtggcg tcattactga
     6661 atccacccct tgcaaacgga cattacgcag aataaatggc atgaccgtgg ttggcagagt
     6721 aaaaccaccc gccagaccac aggcagccac gcagccgccg taattcattt gcgccagcac
     6781 ttttgccagc actttgtcgc caacagtgtc aattgcccca gcccagactt gtttttccag
     6841 aggacgggat tcggcaaact catcacgagg gagaatacga ctggcaccta aacttttcag
     6901 atattcatgg gtactttcgc gaccggaaac ggcaacgacc tgataaccca acttatgcag
     6961 cagcgccacg gcggtactgc cgacgccacc actggcaccc gtcacgacaa tctccccgtc
     7021 ctgcgggcga acaccggcat cttccagcgc catcacacac agcatggcgg taaaaccggc
     7081 agtaccgata atcattgctt tacgcgcgtc cagcccttgc ggcatggcaa ccagccagtc
     7141 acctttcact cgcgcctgct ccgccagccc accccagtgg ttttcaccaa cgccccagcc
     7201 agtgagtaac acctcctgac cggcatgaaa acgcggatct tcgctggtgc gtacagttcc
     7261 ggcaaaatcg atcccaggaa tcatcggaaa attacggatg atttttccct taccggtaat
     7321 cgccagcgca tctttatagt tcaggctcga ccagtgaaca tcaaccgtga catcgtcctc
     7381 cggcaggcga ctttcgtcaa gtgtctgtac cgatgcgaga gttttgccgt cctgctgttc
     7441 taaaagtaac gcctgcataa gtggtcctca tgtgcatgat ggattggaaa ataatttctg
     7501 aagactatac tcgctaatgg aaaagcaaag ccgatgaagc gcaataaatt gcgagataaa
     7561 tctgatttgc tagtatgccc gcttcctcac tatcggagtt aacacaagga tgagattaac
     7621 gacgaaattt tcggcctttg ttacgctgct caccgggtta acaatttttg tgactttgct
     7681 gggctgttcg ctaagtttct acaacgcaat tcagtataag tttagtcatc gcgttcaggc
     7741 ggtggcgacg gcgattgata cccaccttgt gtcgaatgac ttcagcacat taaggccaca
     7801 aattaccgaa ttaatgatgt cggcagatat cgttcgtgta gacctgctcc atggtgataa
     7861 acaggtttat accctggcca gaaatggtag ttatcgtccg gttggctcca gcgatctgtt
     7921 tcgcgaactg agtgttccgt tgataaagca tccggggatg tcgctgcgtc tggtttatca
     7981 ggatccgatg ggcaactatt tccattcttt aatgactacc gcgccgctca cgggggcgat
     8041 tggctttatc attgttatgc tcttcctggc ggtacgctgg ttacaacggc aacttgccgg
     8101 gcaagaattg ctggaaaccc gggctactcg tatcttaaac ggtgagcgtg gctctaatgt
     8161 gttgggaacc atctatgaat ggccgcccag aaccagcagt gcgctagata cgctgcttcg
     8221 tgaaattcag aacgcacgcg aacaacacag ccgtcttgat acgctgatcc gctcttatgc
     8281 cgcccaggac gtgaaaaccg gcctcaataa ccgactcttt ttcgataatc agttagcaac
     8341 gttactggaa gatcaggaga aagtaggtac ccacgggatc gtgatgatga ttcgtctgcc
     8401 ggatttcaat atgttgagcg atacttgggg gcacagccag gttgaagaac agttcttcac
     8461 tctgacgaat ctgctgtcga catttatgat gcgctaccct ggcgcactgc tggcgcgtta
     8521 ccaccgcagt gattttgctg cgctgttacc gcaccggacg ttaaaagagg cagagagcat
     8581 cgccggtcag ttaatcaaag ccgtcgatac cttgccgaac aataaaatgc tcgatcgcga
     8641 cgatatgatc cacattggta tctgcgcctg gcgtagtggt caggataccg agcaggtaat
     8701 ggaacatgca gagtctgcca cgcgtaatgc gggattgcag ggcggcaata gctgggctat
     8761 ttacgatgac tcgttgcctg aaaaaggacg cggtaatgtt cgctggcgta cgcttatcga
     8821 gcaaatgctc agtcgcggcg gcccgcgcct ttatcaaaaa ccggcggtta ctcgcgaagg
     8881 tcaggttcat catcgcgaac tcatgtgccg catcttcgat ggtaatgaag aggttagctc
     8941 ggcggagtat atgccgatgg tcttgcagtt tggcttatcg gaagagtatg accgtctgca
     9001 aatcagccgt cttattccac tattgcgtta ctggccagag gaaaatctgg cgattcaggt
     9061 taccgttgag tcgctgattc gcccgcgttt tcagcgttgg ctgcgcgata cgttaatgca
     9121 atgtgaaaaa tcacaacgaa aacgcataat tattgaactt gcagaggccg atgtaggtca
     9181 acatatcagt cgtttacaac ctgttattcg tttagtgaat gctttagggg tacgggtagc
     9241 cgtcaaccag gctggtttga cgctggtaag caccagttgg atcaaagaac ttaatgttga
     9301 gttactcaag ctccatccgg ggctggtcag aaacattgag aagcgaacgg agaaccagct
     9361 gctggttcaa agcctggtgg aagcctgctc cgggaccagc acccaagttt acgccaccgg
     9421 tgtgcgttcg cgaagcgagt ggcagaccct gattcagcgc ggtgttacag gcgggcaagg
     9481 ggattttttc gcgtcctcac agccacttga tactaacgtg aaaaaatatt cacaaagata
     9541 ctcggtttaa cctgccgttt aatctgtttt cacgtagaat aacgcgcgct gcgtctcatg
     9601 ggagtgtgct tgtctgctcg ccagattgtt gcagcacata tgcagatgaa tgaccttacg
     9661 cggttgcaaa caggcgagga atgctgctga tgcattaagc ctttctggac tcaggcagag
     9721 atttgtaaca aaggaaacga actgcactaa ttttcaccgt agcagatgat ttttgcgcct
     9781 tgtcgctgct gcgtgtggtt ggtaaagtaa gcggattttc ttttccgccc cagctttcag
     9841 gattatccct tagtatgttg aaaaaatttc gtggcatgtt ttccaatgac ttgtccattg
     9901 acctgggtac tgcgaatacc ctcatttatg taaaaggaca aggcatcgta ttgaatgagc
     9961 cttccgtggt ggccattcgt caggatcgtg ccggttcacc gaaaagcgta gctgcagtag
    10021 gtcatgacgc gaagcagatg ctgggccgta cgccgggcaa tattgctgcc attcgcccaa
    10081 tgaaagacgg cgttatcgcc gacttcttcg tgactgaaaa aatgctccag cacttcatca
    10141 aacaagtgca cagcaacagc tttatgcgtc caagcccgcg cgttctggtt tgtgtaccgg
    10201 ttggcgcgac ccaggttgaa cgccgcgcaa ttcgtgaatc cgcgcagggc gctggtgccc
    10261 gtgaagtctt cctgattgaa gaaccgatgg ctgccgcaat tggtgctggc ctgccggttt
    10321 ctgaagcgac cggttctatg gtggttgata tcggtggtgg taccactgaa gttgctgtta
    10381 tctccttgaa cggcgtggtt tactcctctt ccgtgcgcat tggtggtgat cgtttcgacg
    10441 aagctatcat caactatgtg cgtcgtaatt acggttctct gatcggtgaa gccaccgcag
    10501 aacgtatcaa gcacgaaatc ggttcggctt acccgggcga tgaagtccgt gaaatcgaag
    10561 ttcgtggccg taacctggca gaaggtgttc cacgcggttt taccctgaac tccaacgaaa
    10621 tcctcgaagc actgcaggaa ccgctgaccg gtattgtgag cgcggtaatg gttgcactgg
    10681 aacagtgccc gccggaactg gcttccgaca tctccgagcg cggcatggtg ctcaccggtg
    10741 gtggcgcact gctgcgtaac cttgaccgtt tgttaatgga agaaaccggc attccagtcg
    10801 ttgttgctga agacccgctg acctgtgtgg cgcgcggtgg cggcaaagcg ctggaaatga
    10861 tcgacatgca cggcggcgac ctgttcagcg aagagtaatc ggatgcaggc aggggaaatg
    10921 tctgtttacc ctgcctggtc tgatacgaga atacgcataa cttatgaagc caatttttag
    10981 ccgtggcccg tcgctacaga ttcgccttat tctggcggtg ctggtggcgc tcggcattat
    11041 tattgccgac agccgcctgg ggacgttcag tcaaatccgt acttatatgg ataccgccgt
    11101 cagtcctttc tactttgttt ccaatgctcc tcgtgaattg ctggatggcg tatcgcagac
    11161 gctggcctcg cgtgaccaac tagaacttga aaaccgggcg ttacgtcagg aactgttgct
    11221 gaaaaacagt gaactgctga tgcttggaca atacaaacag gagaacgcgc gtctgcgcga
    11281 gctgctgggt tccccgctgc gtcaggatga gcagaaaatg gtgactcagg ttatctccac
    11341 ggttaacgat ccttatagcg atcaagttgt tatcgataaa ggtagcgtta atggcgttta
    11401 tgaaggccag ccggtcatca gcgacaaagg tgttgttggt caggtggtgg ccgtcgctaa
    11461 actgaccagt cgcgtgctgc tgatttgtga tgcgacccac gcgctgccaa tccaggtgct
    11521 gcgcaacgat atccgcgtaa ttgcagccgg taacggttgt acggatgatt tgcagcttga
    11581 gcatctgcca gcgaatacgg atattcgtgt tggtgatgtg ctggtgacct ccggtctggg
    11641 cggtcgtttc ccggaaggct atccggtcgc ggttgtctct tccgtaaaac tcgataccca
    11701 gcgcgcttat actgtgattc aggcgcgtcc gactgcagga ctgcaacgtt tgcgttatct
    11761 gctgctgctg tggggcgcgg atcgtaacgg cgctaacccg atgacgccgg aagaggtgca
    11821 tcgtgttgct aatgaacgtc tgatgcagat gatgccgcag gtattgcctt cgccagacgc
    11881 gatggggcca aagttacctg aaccggcaac ggggatcgct cagccgactc cgcagcaacc
    11941 gacgacagga aatgcagcta ctgcgcctgc tgcgcccaca cagcctgctg ctaatcgctc
    12001 tccacaaagg gctacgccgc cgcaaagtgg tgctcaaccg cctgcgcgtg cgccgggagg
    12061 gcaatagtgg cgagctatcg tagccaggga cgttgggtaa tctggctctc tttcctcatt
    12121 gcgctgttgc tgcaaatcat gccctggccg gataacctga ttgttttccg gccaaactgg
    12181 gtgttactca tcttgttgta ttggatcctg gccttgcctc atcgcgtaaa tgtgggcaca
    12241 ggttttgtga tgggtgccat actggatctg atcagcggct cgacgcttgg cgtacgcgta
    12301 ttggcgatga gcatcattgc ttacctggtg gcgctgaaat accagctttt ccgcaacctc
    12361 gcattatggc agcaggcgct ggtcgtcatg ttgctttcgc tggtggtgga tattattgtt
    12421 ttctgggcag agtttttagt gattaacgtc tctttcagac cggaagtgtt ctggagtagt
    12481 gtagtcaatg gggtgctctg gccgtggatt ttcttgctga tgcgtaaagt ccgtcagcag
    12541 tttgcagtgc aataaaggtt tctatgactt ctctgtattt agcttccggt tctccgcgtc
    12601 gtcaggagtt acttgcgcaa cttggcgtga cctttgaacg tattgttacg ggcattgagg
    12661 agcagcgtca gccgcaggag agcgcgcagc agtatgttgt gcgtctggcg cgcgagaaag
    12721 cacaggcggg tgtcgcgcaa acggcgcagg atctcccggt gctgggtgcg gatactatcg
    12781 ttatcctgaa tggagaagtg ctggagaaac cgcgcgacgc agagcatgcg gcgcagatgt
    12841 tgcgcaaatt atcgggtcag acccatcagg tgatgacggc agtggcgttg gccgacagcc
    12901 agcacattct cgattgcctg gtggtcaccg atgtgacttt cagaacgtta acagacgaag
    12961 acatcgcggg ctatgtcgcc agcggtgaac cgttagataa agcaggtgca tacggtattc
    13021 aggggctggg tggctgtttt gtcaggaaga taaatggcag ctatcacgcc gtagtcggct
    13081 taccgctggt tgaaacgtat gaattattaa gtaattttaa cgcactgcgt gagaaaaggg
    13141 ataaacatga cggctgaatt gttagtaaac gtaacgcctt cggaaacgcg agtggcgtat
    13201 attgatggcg gtattctgca ggaaattcat attgaacgtg aggcgcgacg cggaatagta
    13261 ggcaatatct acaagggtcg tgtaagtcgt gtacttccgg gtatgcaggc ggcttttgta
    13321 gatattgggc tggataaagc cgcgtttctt catgcatccg atatcatgcc gcacaccgaa
    13381 tgtgtggcgg gtgaagaaca aaagcaattc acggtgcgcg acatctcgga actggttcgt
    13441 caggggcaag atctgatggt gcaggtggtg aaagatccgc ttggcactaa aggtgcgcgc
    13501 ctgaccaccg atatcacgct gccttctcgc tatctggtgt ttatgccagg ggcttctcac
    13561 gttggggttt cccaacgtat tgaaagcgaa tcagaacgtg aacgcctgaa aaaagtggtc
    13621 gcagagtatt gcgacgagca gggcgggttt atcatccgta ccgcagcgga aggggttggc
    13681 gaggctgaac tggcctccga tgccgcttat ctgaaacgcg tctggaccaa agttatggag
    13741 cgtaaaaaac gcccgcagac ccgttatcag ctgtacggcg aactggcgct ggcgcagcgt
    13801 gttctgcgtg atttcgccga cgccgaactg gaccgcattc gcgttgactc acgcctgact
    13861 tacgaagcat tgctggagtt tacctcggag tacattcccg agatgaccag caagctggag
    13921 cattacaccg gacgccagcc gattttcgat ctctttgatg tcgaaaacga aatccagcga
    13981 gcgctggaac gcaaagtaga actgaaatcc ggtggttatc tgattatcga ccagaccgaa
    14041 gcgatgacca ccgtggacat caataccgga gcgtttgtcg gtcatcgcaa tctggacgac
    14101 accattttca ataccaatat tgaagcgacg caggctatcg ctcgccagtt acggttgcgt
    14161 aatctgggtg ggattatcat tattgatttc atcgatatga ataatgaaga tcaccgccgc
    14221 cgcgtgctgc actcgctgga gcaggcgttg agcaaagacc gggtgaaaac cagcgttaat
    14281 ggtttttcgg cgctggggct ggtggagatg acgcgtaaac gcacccgcga aagcattgag
    14341 cacgtactgt gtaacgaatg cccaacctgc cacggtcgcg gaacggtgaa aaccgtggaa
    14401 acggtatgct atgaaatcat gcgcgagatt gttcgtgtcc accatgctta cgactccgac
    14461 cgtttcctgg tctatgcatc tccggcagta gctgaagcct tgaaaggcga agagtcacac
    14521 tcgctggcgg aagtggaaat tttcgttggc aaacaggtta aagtacaaat tgaaccgctc
    14581 tataaccagg agcagtttga cgtcgtaatg atgtaaacag atgctgggcc gccatccggc
    14641 aaagggtttt tgagtcacat ttttagcaga caaggagtga cgggtgaggc gattgccggg
    14701 gattttactg cttactggag ccgcgctcgt tgtgatcgct gctctgctgg ttagcggcct
    14761 gcgtattgct ttaccgcatc ttgacgcctg gcgtccggaa atcctcaaca aaatagaatc
    14821 cgcgactggc atgccggtag aagccagtca gctctcagcc agctggcaga attttggccc
    14881 gacgcttgaa gcacacgaca tccgtgcaga actaaaagat ggcggcgaat tttcggttaa
    14941 acgcgttact ctggcgctgg atgtctggca gagcctgtta catatgcgct ggcagtttcg
    15001 cgacctcact ttctggcagc tgcgctttcg caccaacact cctatcacca gcggtggtag
    15061 cgatgacagt ctggaagcca gtcacatcag cgatctgttt cttcgtcaat ttgaccattt
    15121 cgatctccgc gacagtgaag tcagttttct gacgccatcc ggtcagcgcg ccgagctggc
    15181 gatcccacaa ctcacctggc tgaacgatcc acgtcgacac cgtgcggaag gcctggtaag
    15241 cctctccagc cttaccggac agcacggcgt gatgcaggtg cgcatggatt tgcgcgatga
    15301 tgaggggttg ttaagcaatg gtcgcgtctg gctacaggcg gatgacatcg acctgaagcc
    15361 gtggctcggt aaatggatgc aggacaatat tgctctggaa acggcacagt tctcccttga
    15421 aggctggatg acgatcgaca aaggcgatgt aaccggcggt gacgtctggc tgaaacaggg
    15481 cggtgccagc tggttgggcg agaagcaaac gcatacgctg tcggtggata atctgaccgc
    15541 gcatattacg cgtgaaaatc cgggctggca gttctctatt cccgatacac ggatcacgat
    15601 ggacggcaaa ccctggccga gcggagcatt gacgctggcc tggataccgg aacaggacgt
    15661 tggcggcaaa gacaataaac gcagtgacga actccggatt cgcgccagta atctggagct
    15721 ggcaggcctg gagggcatac gcccgctggc cgcgaaactt tcacctgcac tgggtgatgt
    15781 ttggcgctcc acacaaccga gcggcaagat taacactctg gcgctggata tcccgcttca
    15841 ggcggcagac aagacccgtt ttcaggcatc gtggagcgat ctggcctgga agcaatggaa
    15901 attattaccg ggtgcggaac acttctccgg gacgctttcc ggcagcgttg aaaatggttt
    15961 gcttaccgcg tcgatgaagc aggcaaagat gccttacgaa acggtattcc gtgcgccact
    16021 ggaaatcgcc gacggccagg caactataag ctggctgaac aatgacaaag gtttccagct
    16081 ggatgggcgt aatattgacg ttaaagccaa agccgtccat gcgcgcggcg gttttcgtta
    16141 cctgcaacct gctaacgatg aaccctggct gggtattctg gctggcatca gtaccgatga
    16201 tggttcacaa gcctggcgct atttcccgga aaacttgatg ggtaaagacc tggttgatta
    16261 cttaagtggc gcgattcagg gcggtgaagc ggataacgcg acgctggttt atggtggcaa
    16321 tccgcaactc ttcccctata aacacaacga aggtcagttt gaagtgctgg tgccgctgcg
    16381 caacgcgaag tttgccttcc agccggactg gcctgcatta actaaccttg atattgaact
    16441 ggactttatt aacgacggtt tatggatgaa aaccgatggc gttaatctgg gcggcgtgcg
    16501 cgcgagtaat ctcaccgcag tgatccctga ctactcgaaa gaaaaactgc tgattgacgc
    16561 tgacattaaa ggtccgggta aagccgttgg cccttacttt gatgagacac cgctgaaaga
    16621 ttctctgggt gcgaccctgc aagaactcca gctcgacggc gatgtgaatg ctcgcttaca
    16681 tcttgatatc ccgctgaacg gcgaactggt aaccgcgaaa ggtgaagtga cgctgcgtaa
    16741 taacagtctg tttatcaaac cactcgacag caccctgaaa aatttgagcg gtaaattcag
    16801 ctttatcaat ggcgatctgc aaagtgaacc actgacagca agctggttta atcagccgtt
    16861 gaacgtggat ttttccacca aagaaggggc aaaagcctac caggttgcgg tgaatctcaa
    16921 cggtaactgg caaccggcga aaaccagcgt tctgcctgaa gcggtgaacg aagcattgag
    16981 tggcagcgtg gcgtgggatg gtaaagtggg cattgatctg ccttatcatg ctggcgcgac
    17041 ctataacgta gagctgaacg gcgatctgaa taatgtgagt agtcacttac cttcaccgtt
    17101 agccaaacct gcgggtgaac cactagcggt aaacgttaag gttgatggca atctcaacag
    17161 ctttgaatta accggacagg ctggtgcgga taaccatttc aatagccgct ggttgcttgg
    17221 tcaaaagctg acgctcgatc gtgctatttg ggcggcagac agtaaaacgc tcccgccgtt
    17281 gccggaacaa agtggcgttg aactcaatat gccgccgatg aatggtgccg agtggctggc
    17341 cctgttccag aaaggcgctg cggagagtgt cggtggtgca gcgagtttcc cacaacacat
    17401 aacgttacgt acgcctatgt tgtcactggg aaatcagcaa tggaataacc tgagtattgt
    17461 ttcgcaaccg acggcaaatg gcaccctggt tgaggcgcaa gggcgtgaaa tcaacgccac
    17521 gctagcgatg cgtaataacg cgccgtggct ggcgaatatc aaatatcttt attacaaccc
    17581 gagcgtggcg aaaactcgtg gtgattcaac gccgtcatca cctttcccga caacggagcg
    17641 cattaacttc cgtggctggc cggacgcaca aatacgatgc acagagtgct ggttctgggg
    17701 gcaaaaattc ggtcgcattg acagtgatat caccatttct ggcgatacgt taacgctgac
    17761 caatggactg attgatactg gtttctcgcg gcttactgcc gatggtgaat gggttaataa
    17821 tccggagaat gaacgtacct cgctgaaagg aaaactgcgc gggcagaaaa ttgatgccgc
    17881 cgcagaattt tttggtgtca cgacgcccat acgccagtcg tcatttaatg tggattacga
    17941 tttacactgg cgcaaagcac cctggcaacc agatgaggcg acgttgaatg gcatcattca
    18001 tactcaactg ggtaaaggcg aaattaccga aatcaatacc ggacatgccg ggcaattgct
    18061 gcgcttattg agcgttgatg ccctgatgcg taagctgcgt tttgatttca gagacacttt
    18121 tggcgaaggg ttctattttg actccattcg cagcaccgcg tggattaaag acggggttat
    18181 gcacaccgac gacacgctgg tggatggcct ggaggcggat atcgccatga aagggtcggt
    18241 aaatctggta cgtcgcgacc tgaatatgga agcggttgtc gcaccagaga tttctgcgac
    18301 ggtgggcgtg gctgcggctt ttgcggttaa ccccattgtt ggcgcggcag tatttgcagc
    18361 cagtaaagtg ctggggccgc tgtggagcaa agtctccatt ttgcgctatc acatttcggg
    18421 tccgctggac gatccgcaaa tcaacgaagt gttgcgccaa ccgcgtaaag aaaaagcgca
    18481 atgatttgac gagggcgcgt aattgcccca atctcatatg ataatcgttg ccaaaggcca
    18541 acgagccgga acataactgt aggccggata gggcgttcac gccgcatccg gcagccgtaa
    18601 aaaatcctct actgcagtaa ctaacgagta gcaaaaacga tgagtctgaa cctggtaagt
    18661 gaacaattgc tagcggcgaa cggcctgaaa catcaggact tgttcgcgat cctcggtcaa
    18721 ctggccgaac gtcgccttga ttatggcgat ctctattttc agtcgagcta tcacgaatcc
    18781 tgggttttag aagaccgcat tattaaagat ggttcttaca acatcgatca gggcgttggt
    18841 gtgcgtgcaa tcagtggtga aaaaaccgga tttgcttacg ctgaccaaat cagcctgctg
    18901 gcgctggaac agagtgcgca agcggcgcgc accatcgtcc gtgatagtgg tgatggcaaa
    18961 gtacagacgc tgggcgcggt agagcatagc ccgttgtata cctcggtaga tccgctgcaa
    19021 agcatgagcc gtgaagagaa gctggatatc ctgcgtcgcg tcgataaggt tgcccgcgaa
    19081 gcggacaagc gcgtacaaga agtgactgcc agcctcagcg gcgtttatga attaattctg
    19141 gttgcggcca ccgacggcac gctggcggcg gatgttcgtc cgctggtgcg tctttccgtg
    19201 agcgttctgg tcgaagaaga tggcaaacgc gaacgcggtg ccagtggcgg cggcggtcgt
    19261 tttggttatg agttcttcct tgccgatctt gacggcgaag tccgtgcgga tgcatgggca
    19321 aaagaagcag tgcgtatggc gctggtcaat ctttctgccg ttgctgcacc agcgggcacc
    19381 atgccggtag tacttggcgc aggttggccg ggcgtgctgt tgcatgaagc ggtaggtcac
    19441 ggtctggaag gcgacttcaa ctgccgcggc acttcagtat ttagtgggca ggtcggggag
    19501 ctggtggctt cagaactgtg taccgtggtt gatgacggca cgatggtcga tcgccgtggt
    19561 tcggtggcga ttgatgacga aggtacgcca ggccagtaca acgtgctgat tgagaacggc
    19621 attctgaaag gctacatgca ggataaactc aacgcgcgtt tgatggggat gacgccgact
    19681 ggcaacggtc gccgtgaatc ctacgcccat ctgcccatgc cgcgtatgac caacacctat
    19741 atgctgccgg gtaaatcgac cccgcaggaa attattgaat ccgttgagta cggtatctat
    19801 gcaccgaact ttggtggcgg tcaggtggat attacatccg gcaaattcgt tttctccact
    19861 tcagaagcgt atctgattga aaacggtaaa gtaacgaagc cggtgaaagg cgcgacgttg
    19921 attggttccg gtatcgaaac catgcagcag atttcgatgg ttggcaacga cctgaaactg
    19981 gataacggcg tgggtgtctg cggtaaagaa gggcaaagtt tgccggttgg cgtgggccag
    20041 ccaacgctga aagttgataa cctgactgtt ggcggtactg cgtaataatt tcattatttt
    20101 cagaaggata attaatcttt ctacgtgcac gaacggtccc ctcgctcctc tggggttagg
    20161 gtgaggggaa cccgttggca caggtttgta gaacgtaaca gtacaatatg aattacttct
    20221 ctttcccgcg cccgtgcatc tcctgaaaca atttaccgac ctcaacaaaa taatccgtca
    20281 gcgagttgat cacgacctgt accttcagcg gcagcttatc tttttcggta tataacgcat
    20341 aaaccgggcg tggatctgac tgataacgcg gcagcaggat ctccagctca ccgcgattga
    20401 tctcgttgat cacccacatc agcggcacgt aggcgatccc ggcacccgcc gttagccagc
    20461 gcaccagcgt catcggatca ttagtcacaa atctcccttg cgggatcagg cgagtcgaga
    20521 tcccttccgg tgcaatcagt tcaaattcat tgtcgggccg cacgctgtat tcaagccatg
    20581 aatggctact taaatcggcg ggtttttccg gtatgccgta ttgtgtgaga tagcttttcg
    20641 cggcgcacac caccattggc atcgcgccaa gacggcggga aaacaggctg gaatcctgca
    20701 acgcgccgac gcggatcacc acatccagac cgtcggcaat caggtcgggg gctggaattc
    20761 cggtaaccag attgacgctc aaacctgggt attctttcag cattttggct gtcagcccgg
    20821 cgagaacatt ttgtgccata gttgaagaac agccaatgcg tagcgtcccg atgggggtgt
    20881 tattgaaggc atacagttgc tcatgaacat cctgcacttc atgaagcata cgacggcagc
    20941 cctggtagta aattctaccg gcttcggtca ggccaatgct gcgtgtgcta cggtttaaca
    21001 gctttacctg caactcatct tccagttttg acaccgtctg actgatggac gaaacgctca
    21061 tctgtagctg tctggcggcg gcggtaaaag agccaaattc aactactttg gcaaacaccg
    21121 acatgcgttt tagtcgttcc attattcact ctgacttaaa agtgatttag atcacataat
    21181 atagataaca gcataacagt tacgctaata tattaaatat caatctacag caatgttgct
    21241 ctcgcccggc tttccatgcc cttctctgcg gcgacagatg ctgaaaataa taacgcctgc
    21301 tctctctata caaccaaggt caacatgagt ctgtttcccg ttatcgtggt gtttgggctg
    21361 tccttcccac cgatattttt tgaattgctt ttatcactgg cgattttctg gctggtgcgc
    21421 cgggtacttg tgccaacagg tatctacgac tttgtctggc atccggcgtt gttcaacacc
    21481 gcgctctatt gctgcttgtt ttatttgata tcgcgactgt tcgtttgagg ttgaagtgaa
    21541 aacactaata agaaaattct cccgtacggc catcacggtc gtattagtca ttctggcctt
    21601 catcgcaatt tttaatgcct gggtctatta caccgaatcc ccctggacgc gtgacgcgcg
    21661 ctttagcgct gacgtcgttg cgatcgcgcc ggacgtttct ggactcatta cccaggtgaa
    21721 tgttcatgat aaccagctgg tgaaaaaagg acagatactg ttcaccatcg accagccgcg
    21781 ctatcaaaag gcgcttgagg aagcgcaagc cgatgttgcc tattatcagg tactggcaca
    21841 ggagaaacgc caggaggccg gacgtcgtaa ccgtctcggt gtgcaggcga tgtctcgcga
    21901 agagatcgac caggctaaca acgtactaca aacggttctg catcagttag cgaaagcgca
    21961 ggcgacccgc gatctggcaa aactggatct tgaacgcacg gtgatccgcg cgccagcaga
    22021 tggctgggtg accaacctca acgtctatac cggtgagttt attactcgag gatcaacggc
    22081 ggttgcgctg gtgaaacaga actccttcta tgtactggcc tatatggaag aaactaagct
    22141 ggaaggggtg cgtccggggt atcgtgcaga gatcacgccg cttggcagta acaaagtgct
    22201 gaaagggact gttgatagtg ttgccgctgg ggtcaccaac gccagcagca cgcgtgacga
    22261 caaagggatg gcgaccatcg actctaacct tgaatgggtg cgtttggcgc aacgtgttcc
    22321 ggttcgtatt cgtctcgaca accagcaaga gaacatctgg cctgcgggca ccactgctac
    22381 agtggtggtc actggcaaac aagatcgcga cgaaagccag gattcgttct tccgtaaaat
    22441 ggcccatcgc ctgcgtgagt ttggttaatc acgatgggta ttttctccat tgctaaccaa
    22501 catattcgct ttgcggtaaa actggcgacc gccattgtac tggcgctgtt tgttggcttt
    22561 cacttccagc tggaaacgcc acgctgggcg gtactgacag cggcgattgt tgccgccggt
    22621 ccggcctttg ctgcgggagg tgaaccgtat tctggcgcta ttcgctatcg tggctttttg
    22681 cgcatcatcg gcacatttat tggctgtatt gccggactgg tgatcatcat tgcgatgatc
    22741 cgcgcaccat tattgatgat tctggtgtgc tgtatctggg ccggtttttg tacctggata
    22801 tcctcgctgg tacgaataga aaactcgtac gcgtgggggc tggccggtta taccgcgctg
    22861 atcattgtga tcaccattca gccggaacca ttgcttacgc cgcagtttgc cgtcgaacgt
    22921 tgtagcgaga tcgttatcgg tattgtgtgt gcgattatgg cggatttgct cttttctccg
    22981 cgatcgatca aacaagaagt ggatcgagag ctggaaagtt tgctggtcgc gcaatatcaa
    23041 ttaatgcaac tctgtatcaa gcatggcgat ggtgaagttg tcgataaagc ctggggcgac
    23101 ctggtgcgac gcaccacggc gctacaaagc atgcgcagca acctgaatat ggaatcttcc
    23161 cgctgggcgc gggccaatcg acgtttaaaa gcgatcaata cgctatcgct gacgctgatt
    23221 acccaatcct gcgaaactta tcttattcag aatacgcgcc cggaattgat cactgatact
    23281 ttccgcgaat tttttgacac gccggtagaa accgcgcagg acgtccacaa gcagctcaaa
    23341 cgcctgcgga gagttatcgc ctggaccggg gaacgggaaa cgcctgtcac catttatagc
    23401 tgggtcgcgg cggcaacgcg ttatcagctt ctcaagcgcg gcgttatcag taacacaaaa
    23461 atcaacgcca ccgaagaaga gatcctgcaa ggcgaaccgg aagtcaaagt agagtcagcc
    23521 gaacgtcatc atgcgatggt taacttctgg cgaaccacac tttcctgcat tctgggcacg
    23581 cttttctggc tgtggacggg ctggacttcc ggcagtggtg caatggtgat gattgcggtc
    23641 gtgacgtcac tggcaatgcg tttgccgaat ccacgcatgg tggcgatcga ctttatctac
    23701 gggacgctgg ccgcgctgcc gttagggctg ctctactttt tggtgattat ccctaatacc
    23761 caacagagca tgttgctgct gtgtattagc ctggcagtgc tgggattttt cctcggtata
    23821 gaagtacaga aacggcgact gggctcgatg ggggcgctgg ccagcaccat aaatattatc
    23881 gtgctggata acccgatgac tttccatttc agtcagtttc tcgacagcgc attagggcaa
    23941 atcgtcggct gtgtgctcgc gttcaccgtt attttgcttg tgcgggataa atcgcgcgac
    24001 aggaccggac gtgtactgct taatcagttt gtttctgccg ctgtttccgc gatgactacc
    24061 aatgtggcac gtcgtaaaga gaaccacctc ccggcacttt atcagcagct gtttttgctg
    24121 atgaataagt tcccagggga tttgccgaaa tttcgcctgg cgctgacgat gattatcgcg
    24181 caccagcgcc tgcgtgatgc gccgatcccg gttaacgagg atttatcggc gtttcaccga
    24241 caaatgcgcc gcacagcaga ccatgtgata tctgcccgta gcgatgataa acgtcgtcgg
    24301 tactttggcc agttgctgga agaactggaa atctaccagg aaaagctacg catctggcaa
    24361 gcgccaccgc aggtgacgga accggttcat cggctggcgg ggatgctcca taagtatcaa
    24421 catgcgttga ccgatagtta agtcaaaacc gacgccaaaa gcgtcggttt tttcatggct
    24481 atacttagcg atgcacggca gaactcgccg cgaaacgtga cggtggcaac agatgaatat
    24541 ttataccttt gattttgatg agattgagag tcaggaggat ttttatcgtg actttagcca
    24601 aacctttggt ctggctaaag ataaggtacg cgatctcgac tcactatggg atgtgttaat
    24661 gaacgatgtc ctgccgctac cacttgagat tgaatttgtt catctgggag agaaaacgcg
    24721 tcgccgtttt ggcgcgttaa tattgctgtt tgatgaggca gaggaagagc tggaagggca
    24781 tttgcgtttt aatgttcgtc attagcgcaa aaaaaagccc ccgaaccggg ggcaatatcg
    24841 tcggacaaga cgatgagggt ttatttgtac agttcagccg tagcgtgcca ggtgtcaccg
    24901 ctacgagctt cagtaatctg gtaggccgtt gcgcctttct cttccgcttt tttgttcagc
    24961 atttcacgca tatccattgg cgaagacgcc acaccactta cggatacggt cccgattgct
    25021 tcacgatttt gtgcttgtgc agcatcaatg gagtcggcag cgaatgcacc gaaagagaga
    25081 acagaaagta cgcttaatgc agctacagtg gttttgattt tcatgatttt tacctcgaca
    25141 taatctttta gctgggcctt tgtttcgtga cccatatcac aaaatcaagt a
//
LOCUS       DAZKHF020000043        25178 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2201S31241
            SAMN43267800-rid25106413.denovo.043, whole genome shotgun sequence.
ACCESSION   DAZKHF020000043 DAZKHF020000000
VERSION     DAZKHF020000043.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267800
            Sequence Read Archive: SRR30307263
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 25178)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25178)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 25178)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350581.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350581.2
            Genome Coverage       :: 151x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:21:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,652
            CDSs (total)                      :: 5,557
            Genes (coding)                    :: 5,320
            CDSs (with protein)               :: 5,320
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 237
            CDSs (without protein)            :: 237
            Pseudo Genes (ambiguous residues) :: 0 of 237
            Pseudo Genes (frameshifted)       :: 102 of 237
            Pseudo Genes (incomplete)         :: 130 of 237
            Pseudo Genes (internal stop)      :: 52 of 237
            Pseudo Genes (multiple problems)  :: 41 of 237
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25178
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267800-rid25106413.denovo.043"
                     /serotype="O 26:H11"
                     /isolate="2201S31241"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-01"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            complement(82..834)
                     /gene="tcyN"
                     /locus_tag="ACHOSC_RS07130"
                     /old_locus_tag="pgaptmp_001426"
     CDS             complement(82..834)
                     /gene="tcyN"
                     /locus_tag="ACHOSC_RS07130"
                     /old_locus_tag="pgaptmp_001426"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015424 - ABC-type amino acid transporter
                     activity [Evidence IEA];
                     GO_process: GO:0003333 - amino acid transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-cystine ABC transporter ATP-binding protein
                     TcyN"
                     /protein_id="HGU4547400.1"
                     /translation="MSAIEVKNLVKKFHGQTVLHGIDLEVKPGEVVAIIGPSGSGKTT
                     LLRSINLLEQPEAGTITVGDITIDTARSLSQQKSLIRQLRQHVGFVFQNFNLFPHRTV
                     LENIIEGPVIVKGEPKEEATARARELLAKVGLAGKETSYPRRLSGGQQQRVAIARALA
                     MRPEVILFDEPTSALDPELVGEVLNTIRQLAQEKRTMVIVTHEMSFARDVADRAIFMD
                     QGRIVEQGAAKALFADPQQPRTRQFLEKFLLQ"
     gene            complement(831..1499)
                     /gene="tcyL"
                     /locus_tag="ACHOSC_RS07135"
                     /old_locus_tag="pgaptmp_001427"
     CDS             complement(831..1499)
                     /gene="tcyL"
                     /locus_tag="ACHOSC_RS07135"
                     /old_locus_tag="pgaptmp_001427"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cystine ABC transporter permease"
                     /protein_id="HGU4547401.1"
                     /translation="MQESIQLVIDSLPFLLKGAGYTLQLSIGGMFFGLLLGFILALMR
                     LSPIWPVRWLARFYISIFRGTPLIAQLFMIYYGLPQFGIELDPIPSAMIGLSLNTAAY
                     AAETLRAAISSIDKGQWEAAASIGMTPWQTMRRAILPQAARVALPPLSNSFISLVKDT
                     SLAATIQVPELFRQAQLITSRTLEVFTMYLAASLIYWIMATVLSTLQNHFENQLNRQE
                     REPK"
     gene            complement(1514..2500)
                     /gene="dcyD"
                     /locus_tag="ACHOSC_RS07140"
                     /old_locus_tag="pgaptmp_001428"
     CDS             complement(1514..2500)
                     /gene="dcyD"
                     /locus_tag="ACHOSC_RS07140"
                     /old_locus_tag="pgaptmp_001428"
                     /EC_number="4.4.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416429.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019148 - D-cysteine desulfhydrase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-cysteine desulfhydrase"
                     /protein_id="HGU4547402.1"
                     /translation="MPLHNLTRFPRLEFIGAPTPLEYLPRFSDYLGREIFIKRDDVTP
                     MAMGGNKLRKLEFLAADALREGADTLITAGAIQSNHVRQTAAVAAKLGLHCVALLENP
                     IGTTAENYLTNGNRLLLDLFNTQIEMCEALTDPNAQLEELATRVEAQGFRPYVIPVGG
                     SNALGALGYVESALEIAQQCEGAVNISSVVVASGSAGTHAGLAVGLEHLMPESELIGV
                     TVSRSVADQLPKVVNLQQAIAKELELTASAEIILWDDYFAPGYGVPNDEGMEAVKLLA
                     RLEGILLDPVYTGKAMAGLIDGISQKRFKDEGPILFIHTGGAPALFAYHPHV"
     gene            complement(2605..3405)
                     /gene="tcyJ"
                     /locus_tag="ACHOSC_RS07145"
                     /old_locus_tag="pgaptmp_001429"
     CDS             complement(2605..3405)
                     /gene="tcyJ"
                     /locus_tag="ACHOSC_RS07145"
                     /old_locus_tag="pgaptmp_001429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015276 - ligand-gated monoatomic ion
                     channel activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cystine ABC transporter substrate-binding
                     protein"
                     /protein_id="HGU4547403.1"
                     /translation="MKLAHLGRQALMSVMAVALVAGMSVKSFADEGLLNKVKERGTLL
                     VGLEGTYPPFSFQGDDGKLTGFEVEFAQQLAKHLGVEASLKPTKWDGMLASLDSKRID
                     VVINQVTISDERKKKYDFSTPYTISGIQALVKKGNEGTIKTAADLKGKKVGVGLGTNY
                     EEWLRQNVQGVDVRTYDDDPTKYQDLRVGRIDAILVDRLAALDLVKKTNDTLAVTGEA
                     FSRQESGVALRKGNEDLLKAVNDAIAEMQKDGTLQALSEKWFGADVTK"
     gene            complement(3493..4044)
                     /gene="fliZ"
                     /locus_tag="ACHOSC_RS07150"
                     /old_locus_tag="pgaptmp_001430"
     CDS             complement(3493..4044)
                     /gene="fliZ"
                     /locus_tag="ACHOSC_RS07150"
                     /old_locus_tag="pgaptmp_001430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310687.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagella biosynthesis regulatory protein FliZ"
                     /protein_id="HGU4547404.1"
                     /translation="MMVQHLKRRPLSRYLKDFKHSQTHCAHCRKLLDRITLVRDGKIV
                     NKIEISRLDALLDENGWQTEQKSWAALCRFCGDLHCKTQSDFFDIIGFKQFLFEQTEM
                     SPGTVREYVVRLRRLGNHLHEQNISLDPLQDGFLDEILAPWLPTTSTNNYRIALRKYQ
                     HYQRQTCTGLVQKSSSLPASDIY"
     gene            complement(4090..4809)
                     /gene="fliA"
                     /locus_tag="ACHOSC_RS07155"
                     /old_locus_tag="pgaptmp_001431"
     CDS             complement(4090..4809)
                     /gene="fliA"
                     /locus_tag="ACHOSC_RS07155"
                     /old_locus_tag="pgaptmp_001431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor FliA"
                     /protein_id="HGU4547405.1"
                     /translation="MNSLYTAEGVMDKHSLWQRYVPLVRHEALRLQVRLPASVELDDL
                     LQAGGIGLLNAVERYDALQGTAFTTYAVQRIRGAMLDELRSRDWVPRSVRRNAREVAQ
                     AIGQLEQELGRNATETEVAERLGIDIADYRQMLLDTNNSQLFSYDEWREEHGDSIELV
                     TDDHQRENPLQQLLDSNLRQRVMEAIETLPEREKLVLTLYYQEELNLKEIGAVLEVGE
                     SRVSQLHSQAIKRLRTKLGKL"
     gene            complement(4974..6437)
                     /locus_tag="ACHOSC_RS07160"
                     /old_locus_tag="pgaptmp_001432"
     CDS             complement(4974..6437)
                     /locus_tag="ACHOSC_RS07160"
                     /old_locus_tag="pgaptmp_001432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000079822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellin"
                     /protein_id="HGU4547406.1"
                     /translation="MAQVINTNSLSLLTQNNLNKSQSSLSSAIERLSSGLRINSAKDD
                     AAGQAIANRFTANIKGLIQASRNANDGISVAQTTEGALNEINNNLQRVRELTVQATNG
                     TNSDSDLSSIQAEITQRLEEIDRVSEQTQFNGVKVLAENNEMKIQVGANDGETITINL
                     AKIDAKTLGLDGFNIDGAQKATGSDLISKFKATGTDNYDVGGDAYTVNVDSGAVKDTT
                     GNDIFVSAADGSLTTKSDTNIAGTGIDATALAAAAKNKAQNDKFTFNGVEFTTTTAAD
                     GNGNGVYSAEIDGKSVTFTVTDADKKASLITSETVYKNSAGLYTTTKVDNKAATLSDL
                     DLNAAKKTGSTLVVNGATYDVSADGKTITETASGNNKVMYLSKSEGGSPILVNEDAAK
                     SLQSTTNPLETIDKALAKVDNLRSDLGAVQNRFDSAITNLGNTVNNLSSARSRIEDAD
                     YATEVSNMSRAQILQQAGTSVLAQANQTTQNVLSLLR"
     gene            6692..8104
                     /gene="fliD"
                     /locus_tag="ACHOSC_RS07165"
                     /old_locus_tag="pgaptmp_001433"
     CDS             6692..8104
                     /gene="fliD"
                     /locus_tag="ACHOSC_RS07165"
                     /old_locus_tag="pgaptmp_001433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001616262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009288 - bacterial-type flagellum
                     [Evidence IEA];
                     GO_component: GO:0009421 - bacterial-type flagellum
                     filament cap [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar filament capping protein FliD"
                     /protein_id="HGU4547407.1"
                     /translation="MASFTSLGVGSNLPLDTLLNNLTIAEKKRLNPITQQQSDNTARL
                     TAYGTLKSALEKFQTANTALNKADLFKSTTVTSSNEDLKVSTEAGAAPGIYTISVTQL
                     AQAQSLRTDSPTIIASTKDALGDESSDTRTIKITQDGRKEPLEIKLNKDQTSLDEISK
                     AINDADSGISASIVKVKDGNYQLVLTASEGLANKMTISVEGDSKLNDLLAYDSKTNTG
                     NMKELVNAQNAQLNVNGIDIERSSNKITDAPQGVTLDLTKKVTDVRVTVTKSNDKATE
                     AIKGWVDSYNSLIDTFNTLTKYKEVDPGAEAQDKNNGALLGDSVVRTIQSGIRAQFAN
                     GASDSAFKTLNEIGIKQDGTTGKLKIDDDKLKKVLNENTASVRELLVGDGKETGITTK
                     IATEVKGYLADDGIIDSAQDSINATLKKLTKQYLSVSASIDDTVARYTAQFTQLDTMM
                     SKLNNTSTYLSQQFTAMSNS"
     gene            8119..8529
                     /gene="fliS"
                     /locus_tag="ACHOSC_RS07170"
                     /old_locus_tag="pgaptmp_001434"
     CDS             8119..8529
                     /gene="fliS"
                     /locus_tag="ACHOSC_RS07170"
                     /old_locus_tag="pgaptmp_001434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0044780 - bacterial-type flagellum assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar export chaperone FliS"
                     /protein_id="HGU4547408.1"
                     /translation="MYTASGSKAYAQIGVESAVMSASQQQLVTMLFDGVLSALVRARL
                     FMQDNNQQGKGVSLSKAINIIENGLRVSLDEESKDELTQNLIALYSYMVRRLLQANLR
                     NDVSAVEEVEALMRNIADAWKESLLSPSLIQDPV"
     gene            8529..8894
                     /gene="fliT"
                     /locus_tag="ACHOSC_RS07175"
                     /old_locus_tag="pgaptmp_001435"
     CDS             8529..8894
                     /gene="fliT"
                     /locus_tag="ACHOSC_RS07175"
                     /old_locus_tag="pgaptmp_001435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006605 - protein targeting [Evidence IEA];
                     GO_process: GO:0044780 - bacterial-type flagellum assembly
                     [Evidence IEA];
                     GO_process: GO:1902209 - negative regulation of
                     bacterial-type flagellum assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagella biosynthesis regulatory protein FliT"
                     /protein_id="HGU4547409.1"
                     /translation="MNHAPHLYFAWQQLVEKSQLMLRLATEEQWDELIASEMAYVNAV
                     QEIAHLTEEIDPSTTMQEQLRPMLRLILDNESKVKQLLQIRMDELAKLVGQSSVQKSV
                     LSAYGDQGGFVLAPQDNLF"
     gene            8972..10459
                     /gene="amyA"
                     /locus_tag="ACHOSC_RS07180"
                     /old_locus_tag="pgaptmp_001436"
     CDS             8972..10459
                     /gene="amyA"
                     /locus_tag="ACHOSC_RS07180"
                     /old_locus_tag="pgaptmp_001436"
                     /EC_number="3.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_function: GO:0005509 - calcium ion binding [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase"
                     /protein_id="HGU4547410.1"
                     /translation="MRNPTLLQCFHWYYPEGGKLWPELAERADGFNDIGINMVWLPPA
                     YKGASGGYSVGYDSYDLFDLGEFDQKGSIPTKYGDKAQLLAAIDALKRNDIAVLLDVV
                     VNHKMGADEKEAIRVQRVNADDRTQIDEEIIECEGWTRYTFPARAGQYSQFIWDFKCF
                     SGIDHIENPDEDGIFKIVNDYTGEGWNDQVDDELGNFDYLMGENIDFRNHAVTEEIKY
                     WARWVMEQTQCDGFRLDAVKHIPAWFYKEWIEHVQEVAPKPLFIVAEYWSHEVDKLQT
                     YIDQVEGKTMLFDAPLQMKFHEASRMGRDYDMTQIFTGTLVEADPFHAVTLVANHDTQ
                     PLQALEAPVEPWFKPLAYALILLRENGVPSVFYPDLYGAHYEDVGGDGQTYPIDMPII
                     EQLDELILARQRFAHGVQTLFFDHPNCIAFSRSGTDEYPGCVVVMSNGDDGEKTIHLG
                     ENYGNKTWRDFLGNRQESVVTDENGEATFFCNGGSVSVWVIEEVI"
     gene            complement(10493..10906)
                     /gene="yedD"
                     /locus_tag="ACHOSC_RS07185"
                     /old_locus_tag="pgaptmp_001437"
     CDS             complement(10493..10906)
                     /gene="yedD"
                     /locus_tag="ACHOSC_RS07185"
                     /old_locus_tag="pgaptmp_001437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310694.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein YedD"
                     /protein_id="HGU4547411.1"
                     /translation="MKKLAIASALMLLAGCAEVENYNNVVKTPAPDWLAGYWQTKGPQ
                     RALVSPEAIGSLIVTKEGDTLDCRQWQRVIAVPGKLTLMSDDLTNVTVKRELYEVERD
                     GNTIEYDGMTMERVDRPTAECAAALDKAPLPTPLP"
     gene            11093..12298
                     /gene="yedE"
                     /locus_tag="ACHOSC_RS07190"
                     /old_locus_tag="pgaptmp_001438"
     CDS             11093..12298
                     /gene="yedE"
                     /locus_tag="ACHOSC_RS07190"
                     /old_locus_tag="pgaptmp_001438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="selenium metabolism membrane protein YedE/FdhT"
                     /protein_id="HGU4547412.1"
                     /translation="MSWQQFKHAWLIKFWAPIPAVIAAGILSTYYFGITGTFWAVTGE
                     FTRWGGQLLQLFGVHAEEWGYFKIIHLEGSPLTRIDGMMILGMFGGCFAAALWANNVK
                     LRMPRSRIRIMQAIIGGIIAGFGARLAMGCNLAAFFTGIPQFSLHAWFFAIATAIGSW
                     FGARFTLLPIFRIPVKMQKVSAASPLTQKPDQARRRFRLGMLVFFGMLGWALLTAMNQ
                     PKLGLAMLFGVGFGLLIERAQICFTSAFRDMWITGRTHMAKAIIIGMAVSAIGIFSYV
                     QLGVEPKIMWAGPNAVIGGLLFGFGIVLAGGCETGWMYRAVEGQVHYWWVGLGNVIGS
                     TILAYYWDDFAPALATDWDKINLLKTFGPMGGLLVTYLLLFTALMLIIGWEKRFFRRA
                     APQTAKEIA"
     gene            12295..12528
                     /gene="yedF"
                     /locus_tag="ACHOSC_RS07195"
                     /old_locus_tag="pgaptmp_001439"
     CDS             12295..12528
                     /gene="yedF"
                     /locus_tag="ACHOSC_RS07195"
                     /old_locus_tag="pgaptmp_001439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase-like selenium metabolism
                     protein YedF"
                     /protein_id="HGU4547413.1"
                     /translation="MKNIVPDYRLDMVGEPCPYPAVATLEAMPQLKKGEILEVVSDCP
                     QSINNIPLDARNHGYTVLDIQQDGPTIRYLIQK"
     gene            12635..13303
                     /gene="yedK"
                     /locus_tag="ACHOSC_RS07200"
                     /old_locus_tag="pgaptmp_001440"
     CDS             12635..13303
                     /gene="yedK"
                     /locus_tag="ACHOSC_RS07200"
                     /old_locus_tag="pgaptmp_001440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SOS response-associated peptidase"
                     /protein_id="HGU4547414.1"
                     /translation="MCGRFAQSQTREDYLALLAEDIERDIPYDPEPIGRYNVAPGTKV
                     LLLSERDEHLHLDPVFWGYAPGWWDKPPLINARVETAATSRMFKPLWQHGRAICFADG
                     WFEWKKEGDKKQPYFIYRADGQPVFMAAIGSTPFERGDEAEGFLIVTAAADQGLVDIH
                     DRRPLVLSPEAAQEWMRQEIGGKEASEIATNGCVPANQFTWHPVSRAVGNIKNQGAEL
                     IQPV"
     gene            complement(13698..14012)
                     /gene="fliE"
                     /locus_tag="ACHOSC_RS07205"
                     /old_locus_tag="pgaptmp_001441"
     CDS             complement(13698..14012)
                     /gene="fliE"
                     /locus_tag="ACHOSC_RS07205"
                     /old_locus_tag="pgaptmp_001441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009431 - bacterial-type flagellum basal
                     body, MS ring [Evidence IEA];
                     GO_function: GO:0003774 - cytoskeletal motor activity
                     [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-basal body complex protein FliE"
                     /protein_id="HGU4547415.1"
                     /translation="MSAIQGIEGVISQLQATAMSARAQDSLPQPTISFAGQLHATLDR
                     ISDTQTAARTQAEKFTLGEPGVALNDVMTDMQKASVSMQMGIQVRNKLVAAYQEVMSM
                     QV"
     gene            14227..15885
                     /gene="fliF"
                     /locus_tag="ACHOSC_RS07210"
                     /old_locus_tag="pgaptmp_001442"
     CDS             14227..15885
                     /gene="fliF"
                     /locus_tag="ACHOSC_RS07210"
                     /old_locus_tag="pgaptmp_001442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009431 - bacterial-type flagellum basal
                     body, MS ring [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal-body MS-ring/collar protein
                     FliF"
                     /protein_id="HGU4547416.1"
                     /translation="MNATAAQTKSLEWLNRLRANPKIPLIVAGSAAVAVMVALILWAK
                     APDYRTLFSNLSDQDGGAIVSQLTQMNIPYRFSEASGAIEVPADKVHELRLRLAQQGL
                     PKGGAVGFELLDQEKFGISQFSEQVNYQRALEGELSRTIETIGPVKGARVHLAMPKPS
                     LFVREQKSPSASVTVNLLPGRALDEGQISAIVHLVSSAVAGLPPGNVTLVDQGGHLLT
                     QSNTSGRDLNDAQLKYASDVEGRIQRRIEAILSPIVGNGNIHAQVTAQLDFASKEQTE
                     EQYRPNGDESHAALRSRQLNESEQSGSGYPGGVPGALSNQPAPANNAPISTPPANQNN
                     RQQQASTTSNSGPRSTQRNETSNYEVDRTIRHTKMNVGDVQRLSVAVVVNYKTLPDGK
                     PLPLSNEQMKQIEDLTREAMGFSEKRGDSLNVVNSPFNSSDESGGELPFWQQQAFIDQ
                     LLAAGRWLLVLLVAWLLWRKAVRPQLTRRAEAMKAVQQQAQAREEVEDAVEVRLSKDE
                     QLQQRRANQRLGAEVMSQRIREMSDNDPRVVALVIRQWINNDHE"
     gene            15878..16873
                     /gene="fliG"
                     /locus_tag="ACHOSC_RS07215"
                     /old_locus_tag="pgaptmp_001443"
     CDS             15878..16873
                     /gene="fliG"
                     /locus_tag="ACHOSC_RS07215"
                     /old_locus_tag="pgaptmp_001443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009288 - bacterial-type flagellum
                     [Evidence IEA];
                     GO_function: GO:0003774 - cytoskeletal motor activity
                     [Evidence IEA];
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor switch protein FliG"
                     /protein_id="HGU4547417.1"
                     /translation="MSNLTGTDKSVILLMTIGEDRAAEVFKHLSQREVQTLSAAMANV
                     TQISNKQLTDVLAEFEQEAEQFAALNINANDYLRSVLVKALGEERAASLLEDILETRD
                     TASGIETLNFMEPQSAADLIRDEHPQIIATILVHLKRAQAADILALFDERLRHDVMLR
                     IATFGGVQPAALAELTEVLNGLLDGQNLKRSKMGGVRTAAEIINLMKTQQEEAVITAV
                     REFDGELAQKIIDEMFLFENLVDVDDRSIQRLLQEVDSESLLIALKGAEQPLREKFLR
                     NMSQRAADILRDDLANRGPVRLSQVENEQKAILLIVRRLAETGEMVIGSGEDTYV"
     gene            16866..17552
                     /gene="fliH"
                     /locus_tag="ACHOSC_RS07220"
                     /old_locus_tag="pgaptmp_001444"
     CDS             16866..17552
                     /gene="fliH"
                     /locus_tag="ACHOSC_RS07220"
                     /old_locus_tag="pgaptmp_001444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416450.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009288 - bacterial-type flagellum
                     [Evidence IEA];
                     GO_function: GO:0003774 - cytoskeletal motor activity
                     [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar assembly protein FliH"
                     /protein_id="HGU4547418.1"
                     /translation="MSDNLPWKTWTPDDLAPPQAEFVPMVEPEETIIEEAEPSLEQQL
                     AQLQMQAHEQGYQAGIAEGRQQGHKQGYQEGLAQGLEQGLAEAKSQQAPIHARMQQLV
                     SEFQTTLDALDSVIASRLMQMALEAARQVIGQTPTVDNSALIKQIQQLLQQEPLFSGK
                     PQLRVHPDDLQRVDDMLGATLSLHGWRLRGDPTLHPGGCKVSADEGDLDASVATRWQE
                     LCRLAAPGVV"
     gene            17552..18925
                     /gene="fliI"
                     /locus_tag="ACHOSC_RS07225"
                     /old_locus_tag="pgaptmp_001445"
     CDS             17552..18925
                     /gene="fliI"
                     /locus_tag="ACHOSC_RS07225"
                     /old_locus_tag="pgaptmp_001445"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0044780 - bacterial-type flagellum assembly
                     [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar protein export ATPase FliI"
                     /protein_id="HGU4547419.1"
                     /translation="MTTRLTRWLTTLDNFEAKMAQLPAVRRYGRLTRATGLVLEATGL
                     QLPLGATCVIERQNGSETHEVESEVVGFNGQRLFLMPLEEVEGVLPGARVYAKNISAE
                     GLQSGKQLPLGPALLGRVLDGSGKPLDGLPSPDTTETGALITPPFNPLQRTPIEHVLD
                     TGVRPINALLTVGRGQRMGLFAGSGVGKSVLLGMMARYTRADVIVVGLIGERGREVKD
                     FIENILGAEGRARSVVIAAPADVSPLLRMQGAAYATRIAEDFRDRGQHVLLIMDSLTR
                     YAMAQREIALAIGEPPATKGYPPSVFAKLPALVERAGNGISGGGSITAFYTVLTEGDD
                     QQDPIADSARAILDGHIVLSRRLAEAGHYPAIDIEASISRAMTALISEQHYARVRTFK
                     QLLSSFQRNRDLVSVGAYAKGSDPMLDKAIALWPQLEGYLQQGIFERADWEASLQGLE
                     RIFPTVS"
     gene            18944..19387
                     /gene="fliJ"
                     /locus_tag="ACHOSC_RS07230"
                     /old_locus_tag="pgaptmp_001446"
     CDS             18944..19387
                     /gene="fliJ"
                     /locus_tag="ACHOSC_RS07230"
                     /old_locus_tag="pgaptmp_001446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009288 - bacterial-type flagellum
                     [Evidence IEA];
                     GO_function: GO:0003774 - cytoskeletal motor activity
                     [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar export protein FliJ"
                     /protein_id="HGU4547420.1"
                     /translation="MAEHGALATLKDLAEKEVEDAARLLGEMRRGCQQAEEQLKMLID
                     YQNEYRNNLNSDMSAGMTSNRWINYQQFIQTLEKAITQHRQQLNQWTQKVDIALNSWR
                     EKKQRLQAWQTLQERQSTAALLAENRLDQKKMDEFAQRAAMRKPE"
     gene            19384..20511
                     /gene="fliK"
                     /locus_tag="ACHOSC_RS07235"
                     /old_locus_tag="pgaptmp_001447"
     CDS             19384..20511
                     /gene="fliK"
                     /locus_tag="ACHOSC_RS07235"
                     /old_locus_tag="pgaptmp_001447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-length control protein FliK"
                     /protein_id="HGU4547421.1"
                     /translation="MIRLAPLITADVDNATLPGGKASDAAQDFLALLSEALAGETTTD
                     KAAPQLLVATDKPTTKGEPLVSEILADAQQADLLIPVDETPPVINDEQSTSTPLTTAQ
                     TMTLAAVADNNTAKDEKADDLNEDVTASLSALFAMLPGFDNTPKVTDVPSTVLPAEKP
                     TLFTKLTSAQLTTAQPDDAPGTPAQPLTPLVAEAQSKAEVISTPSPVTAAASPLITPH
                     QTQPLPTVAVPVLSAPLGSHEWQQSLSQHISLFTRQRQQSAELRLHPQDLGEVQISLK
                     VDDNQAQIQMVSPHQHVRAALEAALPVLRTQLAESGIQLGQSNISGESFSGQQQAASQ
                     QQQSQRTVNHEPLAGEDDDTLLVPVSIQGRVTGNSGVDIFA"
     gene            20616..21080
                     /gene="fliL"
                     /locus_tag="ACHOSC_RS07240"
                     /old_locus_tag="pgaptmp_001448"
     CDS             20616..21080
                     /gene="fliL"
                     /locus_tag="ACHOSC_RS07240"
                     /old_locus_tag="pgaptmp_001448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009425 - bacterial-type flagellum basal
                     body [Evidence IEA];
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body-associated protein FliL"
                     /protein_id="HGU4547422.1"
                     /translation="MTDYAISKKSKRSLWIPILVFITLAACASAGYSYWHSHQVAADD
                     KAQQRVVPSPVFYALDTFTVNLGDADRVLYIGITLRLKDEATRSRLSEYLPEVRSRLL
                     LLFSRQDAAVLATEEGKKNLIAEIKTTLSTPLVAGQPKQDVTDVLYTAFILR"
     gene            21085..22089
                     /gene="fliM"
                     /locus_tag="ACHOSC_RS07245"
                     /old_locus_tag="pgaptmp_001449"
     CDS             21085..22089
                     /gene="fliM"
                     /locus_tag="ACHOSC_RS07245"
                     /old_locus_tag="pgaptmp_001449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005127760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009288 - bacterial-type flagellum
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor switch protein FliM"
                     /protein_id="HGU4547423.1"
                     /translation="MGDSILSQAEIDALLNGDSEVKDEPTASVSGESDIRPYDPNTQR
                     RVVRERLQALEIINERFARHFRMGLFNLLRRSPDITVGAIRIQPYHEFARNLPVPTNL
                     NLIHLKPLRGTGLVVFSPSLVFIAVDNLFGGDGRFPTKVEGREFTHTEQRVINRMLKL
                     ALEGYSDAWKAINPLEVEYVRSEMQVKFTNITTSPNDIVVNTPFHVEIGNLTGEFNIC
                     LPFSMIEPLRELLVNPPLENSRNEDQNWRDNLVRQVQHSQLELVANFADISLRLSQIL
                     KLKPGDVLPIEKPDRIIAHVDGVPVLTSQYGTLNGQYALRIEHLINPILNSLNEEQPK
                     "
     gene            22086..22499
                     /gene="fliN"
                     /locus_tag="ACHOSC_RS07250"
                     /old_locus_tag="pgaptmp_001450"
     CDS             22086..22499
                     /gene="fliN"
                     /locus_tag="ACHOSC_RS07250"
                     /old_locus_tag="pgaptmp_001450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009288 - bacterial-type flagellum
                     [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0003774 - cytoskeletal motor activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor switch protein FliN"
                     /protein_id="HGU4547424.1"
                     /translation="MSDMNNPADDNNGAMDDLWAEALSEQKSTSSKSAADAVFQQFGG
                     GDVSGTLQDIDLIMDIPVKLTVELGRTRMTIKELLRLTQGSVVALDGLAGEPLDILIN
                     GYLIAQGEVVVVADKYGVRITDIITPSERMRRLSR"
     gene            22502..22867
                     /gene="fliO"
                     /locus_tag="ACHOSC_RS07255"
                     /old_locus_tag="pgaptmp_001451"
     CDS             22502..22867
                     /gene="fliO"
                     /locus_tag="ACHOSC_RS07255"
                     /old_locus_tag="pgaptmp_001451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310713.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0044781 - bacterial-type flagellum
                     organization [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthetic protein FliO"
                     /protein_id="HGU4547425.1"
                     /translation="MNNHATVQSSAPVSAAPLLQVSGALIAIIALILAAAWLVKRLGF
                     VPKRTGVNGLKISASASLGARERVVVVDVEDARLVLGVTAGQINLLHKLPPSAPTEEI
                     PQPDFQSVMKNLLKRSGRS"
     gene            22867..23604
                     /gene="fliP"
                     /locus_tag="ACHOSC_RS07260"
                     /old_locus_tag="pgaptmp_001452"
     CDS             22867..23604
                     /gene="fliP"
                     /locus_tag="ACHOSC_RS07260"
                     /old_locus_tag="pgaptmp_001452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310714.1"
                     /note="The bacterial flagellar biogenesis protein FliP
                     forms a type III secretion system (T3SS)-type pore
                     required for flagellar assembly; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar type III secretion system pore protein
                     FliP"
                     /protein_id="HGU4547426.1"
                     /translation="MRRLLSVAPVLLWLVTPLAFAQLPGITSQPLPGGGQSWSLPVQT
                     LVFITSLTFIPAILLMMTSFTRIIIVFGLLRNALGTPSAPPNQVLLGLALFLTFFIMS
                     PVIDKIYVDAYQPFSEEKISMQEALEKGAQPLREFMLRQTREADLGLFARLANTGPLQ
                     GPEAVPMRILLPAYVTSELKTAFQIGFTIFIPFLIIDLVIASVLMALGMMMVPPATIA
                     LPFKLMLFVLVDGWQLLVGSLAQSFYS"
     gene            23614..23883
                     /gene="fliQ"
                     /locus_tag="ACHOSC_RS07265"
                     /old_locus_tag="pgaptmp_001453"
     CDS             23614..23883
                     /gene="fliQ"
                     /locus_tag="ACHOSC_RS07265"
                     /old_locus_tag="pgaptmp_001453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013365797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0044780 - bacterial-type flagellum assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis protein FliQ"
                     /protein_id="HGU4547427.1"
                     /translation="MTPESVMMMGTEAMKVALALAAPLLLVALVTGLIISILQAATQI
                     NEMTLSFIPKIIAVFIAIIIAGPWMLNLLLDYVRTLFTNLPYIIG"
     gene            23892..24677
                     /gene="fliR"
                     /locus_tag="ACHOSC_RS07270"
                     /old_locus_tag="pgaptmp_001454"
     CDS             23892..24677
                     /gene="fliR"
                     /locus_tag="ACHOSC_RS07270"
                     /old_locus_tag="pgaptmp_001454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006605 - protein targeting [Evidence IEA];
                     GO_process: GO:0044780 - bacterial-type flagellum assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthetic protein FliR"
                     /protein_id="HGU4547428.1"
                     /translation="MMQVTSDQWLSWLSLYFWPLLRVLALISTAPILSERSVPKRVKL
                     GLAMMITFAIAPSLPANDVPVFSFFALWLAVQQILIGIALGFTMQFAFAAVRTAGEII
                     GLQMGLSFATFVDPASHLNMPVLARIMDMLALLLFLTFNGHLWLISLLADTFHTLPIG
                     GEPLNSNAFLALTKAGSLIFLNGLMLALPLITLLLTLNLALGLLNRMAPQLSIFVIGF
                     PLILTVGISLMAALMPLIAPFCEHLFSEMFNLLADIISELPLI"
     gene            24967..>25178
                     /gene="rcsA"
                     /locus_tag="ACHOSC_RS07275"
                     /old_locus_tag="pgaptmp_001455"
     CDS             24967..>25178
                     /gene="rcsA"
                     /locus_tag="ACHOSC_RS07275"
                     /old_locus_tag="pgaptmp_001455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310717.1"
                     /note="with RcsB is a positive regulator of capsular
                     polysaccharide synthesis genes; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator RcsA"
                     /protein_id="HGU4547429.1"
                     /translation="MSTIIMDLCSYTRLGLTGYLLSRGVKKREINDIETVDDLAIACD
                     SQRPSVVFINEDCFIHDASNSQRIKL"
BASE COUNT         6172 a         6457 c         6468 g         6081 t
ORIGIN      
        1 gctgatttgt taagcagttt tatcacgctt gaaatagcgt cccgccccga caggtgaatc
       61 gtcggggctg attttttctt attattgcag cagaaacttc tcgaggaact ggcgggtgcg
      121 aggctgctgg ggatcggcaa ataacgcttt tgctgccccc tgctcgacta tccgcccctg
      181 gtccataaaa atcgcccggt ccgcaacatc ccgggcaaag ctcatttcgt gcgtcacaat
      241 caccatcgtg cgcttttcct gcgccagctg acggatggtg ttcaggactt cgcccaccag
      301 ctctggatcc agcgccgacg ttggctcgtc aaacaaaatc acctcaggac gcattgccag
      361 cgcacgtgca atcgcaacac gttgctgttg accgccagac aaacgacgtg gatagctggt
      421 ttctttaccg gccagcccaa ctttcgccaa cagctcgcga gcgcgcgccg tggcctcttc
      481 tttcggttcg cctttgacga tcaccggccc ttcaataatg ttttccagca ccgtacgatg
      541 cggaaacaaa ttaaagttct ggaagacaaa cccgacgtgc tgacgcaact ggcgaatcag
      601 cgatttttgc tgacttaatg aacgtgcagt atcaatagtg atatcgccaa cggtgatcgt
      661 tcccgcttcc ggttgttcca gcagatttat gctgcgtagc aacgtggttt tgccggaacc
      721 actcggaccg ataattgcca ccacttcgcc aggctttacc tcgaggtcga taccgtgcag
      781 caccgtctga ccgtgaaatt ttttcaccaa gttcttaact tcaatggcac tcattttggt
      841 tctctctcct ggcgattaag ttgattctca aaatggttct gcaacgtcga taatactgtc
      901 gccatgatcc agtagatgag cgaagccgcc aggtacatgg tgaacacctc cagcgtgcgc
      961 gaggtaatca actgcgcctg acggaacagc tccggcacct ggattgtcgc ggccagcgac
     1021 gtatccttta ccaggctgat aaagctgttc gacagcggcg gcaacgccac ccgcgccgcc
     1081 tgtggcaaaa tcgcgcgacg catagtctgc catggcgtca taccaatact ggcagctgct
     1141 tcccactgac ctttatcaat agaagaaatg gcggcgcgca gtgtttcggc ggcataggcg
     1201 gcagtgttca gcgacagacc aatcattgct gacggaatgg gatctaattc gataccaaac
     1261 tgcggcaggc cgtaatagat cataaacagt tgtgcgatga gcggtgtacc acgaaaaata
     1321 gagatataaa aacgcgccag ccagcgcacg ggccagattg gcgacaggcg catcagcgcg
     1381 agaataaacc cgagcagtaa gccaaaaaac atgccgccga tactgagttg cagcgtatac
     1441 ccagcccctt tcagcaggaa cggcaaggaa tcaataacca gttgtatact ttcttgcatg
     1501 agcgttcccg gagctaaacg tggggatgat aggcgaacag cgcaggcgca ccgccggtat
     1561 gaataaacag aatcggccct tcatctttga agcgtttctg actgataccg tcaatcagcc
     1621 ccgccatcgc ttttccggta tacacaggat caagcagaat gccttccagc cgcgccagca
     1681 atttcactgc ttccatgcct tcgtcgttcg gcacgccgta gccaggtgca aaatagtcat
     1741 cccagagtat aatttccgct gatgcggtca gctccagttc tttcgcaatc gcctgttgta
     1801 ggttaaccac tttcggcaac tgatcggcaa cggaacgcga cacggtcacg ccaatcagtt
     1861 cgctttcagg catcaggtgt tccagcccaa cagccagtcc ggcgtgagtt ccggcactgc
     1921 ccgatgcgac taccaccgac gaaatattaa ccgccccttc acactgttgc gcgatttcca
     1981 gcgcactctc cacataacct agcgcgccaa gagcattaga accgccaacc ggaatgacat
     2041 acgggcgaaa gccttgtgct tcgactcgcg tcgccagctc ttccagttgg gcattgggat
     2101 cggtcagtgc ttcgcacatt tcaatctggg tattgaacag atccagcaac aagcgattgc
     2161 cgttggttaa atagttttct gcggttgtgc caataggatt ttccagcagc gccacacagt
     2221 gcagaccgag tttcgccgca actgcggcag tctgccgcac atggttagac tggatcgccc
     2281 cggcagtaat cagcgtatcg gcaccttcgc gcagagcatc tgctgcgaga aattccagct
     2341 tacgtaattt attgccgccc attgccatgg gggtgacgtc atcccgtttg atgaaaattt
     2401 cccgtccaag ataatcagaa aagcgcggca gatattcgag cggtgttggc gcgccgataa
     2461 actccaaccg tggaaaacgg gttaaattat gcagtggcat aacagcctcc gatgtgtgtt
     2521 gttgtgattt tcttattatg cacgctgaaa acgcgtaaat aaaaaaggcg ctggtgaaag
     2581 cgcccttttt tgtcattatg ctgattattt ggtcacatca gcaccaaacc atttttcgga
     2641 aagggcttgc agagtgccat ctttttgcat ttccgcaatt gcatcgttca ctgctttcag
     2701 caggtcttca tttcctttac gcagcgccac gccagactcc tgacgggaga atgcttcacc
     2761 ggttactgcc agcgtatcgt tggttttctt caccagatcc agcgccgcca gacggtcaac
     2821 gaggatcgca tcgatacggc ctacgcgcag atcctgatat ttggtcgggt catcatcata
     2881 ggtacgcaca tcgacgccct gaacattctg ccgcagccac tcttcatagt tggtgcccag
     2941 accgacaccc acttttttgc ctttcagatc agcagccgtt ttaatggtgc cttcgttacc
     3001 ttttttcacc agcgcctgaa taccagaaat ggtgtacggg gtagagaaat cgtatttttt
     3061 cttgcgctca tcagaaatgg tgacctgatt aatcaccaca tcaatgcgtt tagagtccag
     3121 cgacgccagc ataccgtccc atttggtcgg ttttagtgac gcctcaacgc caagatgttt
     3181 tgccagctgt tgggcaaatt ccacttcaaa accggttaat ttgccgtcat ccccctgaaa
     3241 actgaacggc ggataagtgc cttccagccc taccagcagc gtgccgcgct ctttaacttt
     3301 attaagcaga ccttcatctg caaaactttt aacgctcatg cccgcaacca gcgctacggc
     3361 catcacactc atcaatgcct gacgtcccag atgtgctaat ttcatattta ccccgaatgt
     3421 tgttatgtct gtttgcagtg tagagccatt gtttgtaaac acaaaaacaa ctccgctaca
     3481 tcttattctt atttaatata tatcagaagc aggcaggctg gaggatttct gcacaagtcc
     3541 ggtacacgtt tgacgctgat agtgttgata cttccgtaat gcgatgcggt aattgttggt
     3601 gctggtggtg ggcagccacg gggcgaggat ttcatcaagg aaaccgtcct gcagcggatc
     3661 gagggaaata ttttgctcgt gtagatgatt ccccaaacgg cgcaaacgaa cgacatattc
     3721 acgcaccgta cctgggctca tttcagtttg ctcaaaaaga aattgcttaa agccgataat
     3781 atcgaaaaaa tcactctgcg ttttgcaatg taaatcaccg caaaatcggc acaatgccgc
     3841 ccatgatttt tgttccgttt gccagccatt ttcatcaagc agcgcgtcca ggcgggaaat
     3901 ctcgatttta ttcactattt tgccgtcgcg aacgagggta atgcgatcga gtaatttacg
     3961 gcaatgcgcg caatgagtct ggctgtgttt aaagtcttta agatagcggc ttaatggccg
     4021 tcttttcagg tgctgcacca tcattaagaa ctcctggtag tcaaagttaa agtgcggcat
     4081 ttactgacgt tataacttac ccagtttagt gcgtaaccgt ttaatagcct ggctgtgtaa
     4141 ctgactgacc cgcgattccc cgacctccag caccgcgcca atctctttga gattcagctc
     4201 ttcctggtaa tagagggtta ataccagttt ttcgcgctcc ggcaacgttt cgatggcttc
     4261 catcacccgc tgacgcagat tactgtccag cagttgttgt agcgggtttt ctcgctgatg
     4321 atcatcagta accagttcga tgctatcgcc gtgctcttcg cgccactcat cgtaggagaa
     4381 gagctggctg ttattggtgt cgagcaacat ttggcgataa tcggcaatat cgatccctaa
     4441 acgttccgct acctcagttt ccgtggcgtt gcggccaagt tcctgctcca gttgccctat
     4501 tgcctgtgcc acttcacgcg cgttgcgtcg cacgctgcgc ggcacccagt cacggctgcg
     4561 aagttcatcc agcatagcgc cacggatacg ctgcactgcg taagttgtaa atgccgttcc
     4621 ttgtagggcg tcatagcgtt cgacggcatt aagtaaccca atgccgcccg cctgtagcag
     4681 atcgtcaagt tccacgctcg cgggcagtcg aacctgcagg cgcaatgctt cgtgacgcac
     4741 cagcgggaca taacgctgcc atagcgagtg tttatccatt acaccttcag cggtatagag
     4801 tgaattcacg ataaacagcc ctgcgttata tgagttatcg gcatgattat ccgtttctgc
     4861 agggttttta atcggacgat tagtgggtga aatgaggggt tatttggggg ttaccggtaa
     4921 attgcgggca gaaaaaaccc cgccggtggc gggggttgag cgataagtgt aaattaacgc
     4981 agcagagaca gtacgttctg cgtggtctgg ttagcctgcg ccagaacaga ggtacccgct
     5041 tgttgcagga tctgcgcacg agacatgtta gacacttcgg tcgcgtagtc agcatcttcg
     5101 atacggctac gggcagaaga caggttgttt acggtgttgc caaggttggt gatagcagag
     5161 tcgaaacggt tttgtactgc accgaggtca gaacgcagat tgtcaacttt agccaatgct
     5221 ttgtcgatag tttcgagcgg gttggtggta gattgcaacg attttgctgc atcttcgttt
     5281 accagaatcg ggctaccacc ttctgatttg ctcagataca tgactttatt gttaccagaa
     5341 gcagtctccg ttatcgtttt accatctgca ctaacatcgt aagttgcacc gttaacaact
     5401 aacgtgcttc ctgttttctt agctgcattg agatcaagat cggaaagtgt ggcagccttg
     5461 ttatcaactt tggttgtcgt ataaaggcca gcgctatttt tgtaaactgt ctcactcgta
     5521 atcaaagaag cttttttgtc agcatctgtc acagtaaatg tcactgactt accatcaatt
     5581 tctgcagaat atacaccatt cccattgcca tccgctgcag ttgttgttgt gaattcaact
     5641 ccattaaacg tgaatttatc attctgtgct ttattcttag ccgctgctgc gagtgctgta
     5701 gcatcaatcc ctgtaccagc tatgtttgtg tcagatttag ttgtcagtga accatctgct
     5761 gcactaacaa aaatatcatt ccctgtagta tctttaacag ctccgctatc tacgttaaca
     5821 gtataagcat caccgccaac atcatagtta tcagtacctg tcgctttaaa tttagaaatc
     5881 aggtcactgc cagttgcttt ctgcgcgcca tcgatattaa aaccgtccag gccgagagtt
     5941 ttcgcatcaa tttttgccag attgatagtg atggtttcac catcattagc accaacctga
     6001 attttcattt cattattttc agcaaggact ttcacgccgt taaactgagt ttgctcagat
     6061 acacggtcaa tttcttccag acgttgagta atttcagcct ggatagaaga aagatcgctg
     6121 tcagagttag taccgttagt tgcctgaaca gtcagttcac gtacacgctg caggttgttg
     6181 ttaatttcat tcagcgcacc ttcagtggtc tgcgcaacag aaataccatc attcgcgtta
     6241 cgggaagcct gaatcagacc tttaatattt gctgtaaaac ggttagcaat cgcctgacct
     6301 gctgcgtcat ctttagcact gttaatacgc aggccagaag agagacgttc aatggcggag
     6361 ctcagagaag actgagattt gttcaggtta ttctgggtca acagcgacag gctgtttgta
     6421 ttaatgactt gtgccatgat cttttcctta tcaattacaa cttgatgtta ttgggctgtt
     6481 gcccacggtt tctcaccgta ccccttgtat cggcacctga atttcgaact ttagaaaatt
     6541 ttagtttctc tcctgttttt tcttaacggc tgaaatatcc gtaatataca actctactct
     6601 gcgcatcatc ttcgtgaatc aactattaaa ctttgcgcac aaattgccga taacccgctt
     6661 atctactgtt tgcaatcaaa aggaattagg tatggcttca tttacatctc ttggcgttgg
     6721 ttcgaatctg ccgctggata cattgctaaa caacctgacc atcgctgaaa aaaaacgctt
     6781 aaacccaatt actcagcaac agagtgacaa taccgcacgt ctgacagcct atggcacatt
     6841 aaaaagtgca ttagaaaaat tccagactgc aaatactgcg ttaaataaag ccgatctgtt
     6901 caaaagcact accgttacca gtagcaatga agaccttaaa gtttccaccg aagcaggtgc
     6961 tgcccccggt atctatacca tcagcgtaac ccaactggcg caggcgcaat ctttacgaac
     7021 tgattccccg acaatcattg catcaacaaa agatgcactg ggtgatgagt catcagatac
     7081 acgcacaata aaaattaccc aagacggtcg taaagagccg ctggaaatca agctcaataa
     7141 agatcagacg tcattggatg agatcagcaa agccattaac gatgccgata gtggaatttc
     7201 cgctagtatc gttaaagtca aagatggtaa ctatcaattg gtattgaccg ccagtgaagg
     7261 tctggccaat aaaatgacca tatctgttga gggtgacagt aaattaaatg atctgctggc
     7321 ttatgacagc aagaccaaca ccgggaatat gaaggaactg gttaacgcgc aaaatgcaca
     7381 gctaaacgtt aacggtatcg atattgaacg ttcaagtaac aaaattaccg atgctcctca
     7441 gggagtgact ctcgatttga ccaaaaaagt cactgacgtc cgggtcacgg tgacaaaaag
     7501 caacgataaa gcaaccgaag caattaaagg ctgggtggat tcatataact ccctcattga
     7561 cacctttaac accctgacaa aatataaaga agtagaccca ggtgctgaag cacaagataa
     7621 aaataatggt gcacttctcg gagacagcgt tgtacgtaca attcaaagcg gcatccgcgc
     7681 acaatttgca aacggtgcca gcgacagtgc cttcaaaaca ttaaacgaga tcgggattaa
     7741 gcaggacggc acgacgggta agctgaaaat tgatgatgat aaactcaaaa aagtcctgaa
     7801 tgaaaataca gcatccgtac gtgaattgct ggtaggcgat ggtaaagaaa caggaattac
     7861 caccaaaatt gccaccgagg taaaaggtta ccttgccgat gatggcatta ttgatagcgc
     7921 acaggacagt attaacgcca cactgaaaaa actgaccaaa cagtatctat ccgtcagtgc
     7981 cagcattgat gacaccgtgg cgcgttacac tgcacagttt acgcaattag ataccatgat
     8041 gagcaaactg aataacacca gtacctatct cagccaacaa tttactgcaa tgagtaattc
     8101 ctgatcacgg aggtcagcat gtataccgcg agcggctcaa aagcctatgc acaaattggc
     8161 gtcgaaagcg ccgtaatgag cgccagccag cagcagctgg tcaccatgct atttgatgga
     8221 gtgctgagcg cactggttag agcacgcctg tttatgcagg acaacaatca gcaaggcaaa
     8281 ggcgtctctt tgtcaaaagc gatcaacatc attgagaacg gactgcgggt gagtcttgat
     8341 gaagagagca aagacgaatt aacccaaaac ttgattgctc tttatagcta tatggtcagg
     8401 cgcttgctgc aagccaattt acgcaacgat gtctccgcag tcgaagaagt ggaagcatta
     8461 atgcgcaata ttgccgatgc ctggaaagag tcgttactct ccccttcttt gattcaggac
     8521 ccagtctgat gaaccacgca ccgcatttgt atttcgcctg gcaacaactc gtcgaaaaaa
     8581 gccagctcat gttacgcctg gcaacagaag aacaatggga cgaactcatc gccagcgaga
     8641 tggcgtatgt gaatgcggtg caggagattg cacatctgac tgaagagatt gacccgtcca
     8701 ccacgatgca ggagcagctc cgcccgatgc tgcgcctgat tctcgacaac gaaagcaagg
     8761 taaagcagtt attacagatt cggatggatg aactggcgaa actggtcggt cagtcatcgg
     8821 tgcaaaaatc ggtgttaagt gcctatggtg atcagggcgg ctttgtgctg gctccgcagg
     8881 ataacctctt ttgaatctga acgagtcgat ggctcgcgaa taatccgatt acggctacgc
     8941 ttctaatgtt ccccttgaat ggagtcgaag aatgcgtaat cccacgctgt tacaatgttt
     9001 tcactggtat tacccggaag gcggtaagct ctggcctgaa ctggccgagc gcgccgacgg
     9061 ttttaatgat attggtatca atatggtctg gttgccgccc gcctataaag gcgcatcggg
     9121 cgggtattcg gtcggctacg actcctatga tttatttgat ttaggcgagt ttgatcagaa
     9181 aggcagcatc cctactaaat atggcgataa agcacaactg ctggccgcca ttgacgccct
     9241 gaaacgtaat gacattgcgg tgctgttgga cgtggtagtc aaccacaaaa tgggcgcgga
     9301 tgaaaaagaa gctattcgcg tgcagcgtgt aaatgctgat gaccgtacgc aaattgacga
     9361 agaaatcatt gagtgtgaag gctggacgcg ttacaccttc cccgcccgtg ccgggcaata
     9421 ctcgcagttt atctgggatt tcaaatgttt tagcggtatc gaccatatcg aaaaccctga
     9481 tgaagatggc atatttaaaa ttgttaacga ctacaccggc gaaggctgga acgatcaggt
     9541 tgatgatgaa ttaggtaatt tcgattatct gatgggcgag aatatcgatt ttcgcaatca
     9601 tgccgtgacg gaagagatta aatactgggc gcgctgggtg atggaacaaa cgcaatgcga
     9661 cggttttcgt cttgatgcgg tcaaacatat tccggcgtgg ttttataaag agtggatcga
     9721 acacgtacag gaagttgcgc caaagccgct gtttattgtg gcagagtact ggtcgcatga
     9781 agtcgataaa ctgcaaacgt atattgatca ggtggaaggc aaaaccatgc tgttcgatgc
     9841 gccgctgcag atgaaattcc atgaagcatc gcgcatgggg cgcgactacg acatgacgca
     9901 gattttcacg ggtacattag tggaagccga tcctttccac gccgtgacgc tcgttgccaa
     9961 tcacgacacc caaccattgc aagccctcga agcaccggtc gaaccgtggt ttaaaccgct
    10021 ggcgtatgct ctaattttgt tgcgggaaaa tggcgttcct tcggtgttct atccggacct
    10081 ctacggtgcg cattacgaag atgtcggtgg tgacgggcaa acctatccga tagatatgcc
    10141 aataatcgaa cagcttgatg agttaattct cgcccgtcag cgtttcgccc acggtgtaca
    10201 gacgttattt ttcgaccatc cgaactgcat tgcctttagc cgcagtggca ctgacgaata
    10261 tcccggctgc gtggtggtca tgtcgaacgg ggatgatggc gaaaaaacca ttcatctggg
    10321 agagaattac ggcaataaaa cctggcgtga ttttctgggg aatcggcaag agagcgtagt
    10381 gaccgacgaa aacggcgaag cgaccttctt ttgcaacggc ggcagcgtca gcgtgtgggt
    10441 tatcgaagag gtgatttaaa ttcatccccg gcggcaagcc ggggagattt cattacggca
    10501 gtggcgtcgg taacggcgct ttatccagcg cggcggcaca ctctgccgtc gggcgatcca
    10561 cacgctccat cgtcatacca tcgtactcaa tggtgttacc gtcacgctca acctcgtaca
    10621 gctcacgttt tacggtaacg ttagtgagat catcagacat caatgtcaac ttgcccggca
    10681 cggcaatcac ccgctgccac tgacggcaat ccagggtatc gccctcttta gtgacaatca
    10741 ggctgcctat cgcttccgga ctgaccagcg cacgttgcgg ccctttggtc tgccagtagc
    10801 ccgctagcca gtccggcgcg ggggttttca cgacattgtt atagttttcg acttcggcac
    10861 agcccgccag caacatgagc gcgcttgcaa ttgcgagttt tttcatcatt cgtcctgcat
    10921 gagaagaaaa gaggattgtg gcattaaagc cctgatgtcg ccagcctttc caggttcaga
    10981 gagaaatttg atctaactat tatttttgtg aattattacc ccgaaaataa atttgcatga
    11041 tgtatgattc gcgattcttg tttcaaacct tctgagttca gaggctacat tcatgtcatg
    11101 gcagcaattc aaacacgcct ggttgattaa attctgggca cccatccctg cggtcatcgc
    11161 ggcgggtatt ctctccactt actattttgg cattactggc accttttggg ctgtcacggg
    11221 tgaatttacc cgttggggcg gccagctcct gcagctgttt ggcgtccatg ctgaagagtg
    11281 gggttatttt aaaattatcc atctggaagg atcgccatta acccgcatcg acgggatgat
    11341 gatcctcggc atgtttggcg gctgctttgc cgcagcgctg tgggccaaca atgtcaaact
    11401 gcgcatgcca cgcagccgta tccgcattat gcaggccatt attggcggta ttatcgctgg
    11461 ttttggcgcg cgtctggcaa tgggctgtaa cctggcggcg ttctttaccg gtattcctca
    11521 gttctcgctg catgcctggt tctttgccat cgccactgcc attggttcat ggtttggcgc
    11581 acgctttacc ctgctgccca tcttccgtat tcccgtgaaa atgcagaaag tttctgccgc
    11641 ctcaccgctg acgcaaaaac cggatcaggc gcggcgtcgt tttcgtctcg ggatgctggt
    11701 ctttttcggt atgctgggct gggcgctgct cacggcgatg aaccaaccaa aactggggct
    11761 ggcaatgctg tttggcgtcg gctttggttt actgattgaa cgtgcgcaaa tctgctttac
    11821 ttcggcgttc cgcgatatgt ggatcaccgg acgtacccat atggcgaaag caatcattat
    11881 cggtatggcg gtgagtgcca tcgggatctt cagttacgta cagttaggcg ttgaacccaa
    11941 aatcatgtgg gcgggaccaa acgcggtaat tggtggttta ctgtttggtt ttggcatcgt
    12001 gctggcaggc ggctgcgaaa ccggctggat gtaccgcgcg gtagaaggcc aggtgcacta
    12061 ctggtgggtc ggtctgggca atgtgatcgg ctcgacgatt ctggcgtatt actgggatga
    12121 tttcgctccg gcgctggcca ccgactggga caaaatcaac ctgctgaaaa cctttggccc
    12181 gatgggtggc ctgctggtga catatttgct gttgtttact gcgctgatgt tgattatcgg
    12241 ctgggaaaaa cgcttcttcc gccgtgcggc accgcagact gctaaggaga tcgcatgaaa
    12301 aatatcgttc ctgattaccg ccttgatatg gtgggtgaac cctgccctta tccggcagtc
    12361 gcaactcttg aggcgatgcc gcagttaaaa aaaggggaaa tcctggaagt ggtgagcgac
    12421 tgtccacagt cgatcaataa tattccactg gatgcgcgta atcacggcta tacggtgctg
    12481 gatattcagc aagacgggcc gaccattcgt tatttaattc agaagtaatc cctcattccc
    12541 gctgtggccc acagcgggaa catcgttgcc tctgacacat agattttacc cgccccctct
    12601 cctattcttt tcagtatgat ctcaggaggt ttctatgtgt ggacgctttg cccaatccca
    12661 aacgcgtgaa gattaccttg cgcttctcgc ggaagatatt gaacgcgata ttccctacga
    12721 tcccgaaccc attggcagat acaacgtcgc gccgggaacc aaagttcttc tgctcagtga
    12781 acgtgatgaa caccttcatc tggatccggt tttctgggga tatgctcccg gatggtggga
    12841 taaaccgccg ctgattaacg cccgcgtaga aactgcggcc accagtcgta tgtttaaacc
    12901 gctctggcaa catggtcggg caatctgttt tgccgatggc tggtttgagt ggaaaaaaga
    12961 aggcgacaaa aaacagcctt attttatcta tcgcgctgat ggacaacctg tttttatggc
    13021 cgcgataggt agcacaccgt ttgagcgcgg tgacgaagcc gaaggatttt taattgtcac
    13081 tgcagcagca gaccagggtc tggtagatat tcatgaccgc cgcccgctgg ttctgtcgcc
    13141 agaagctgca caggaatgga tgcggcaaga gattggcggt aaagaagcat cagaaattgc
    13201 taccaacggc tgtgtgccag caaaccagtt cacctggcac ccggtatcgc gcgcggtggg
    13261 taatatcaaa aaccagggag cggagttaat tcaaccggtt tgataagcca ggagactatt
    13321 gatcaataat actgcgtcat aagaaatctc tattagacaa tagtagacgc tccttccaca
    13381 tgcgccacaa gaaacctcta ttccagtgac acaattacgc ctgattattt ccctagcccg
    13441 gaatatgaat aaaaacatca acaaagatta aattattcaa acgttactgt tgcgtctgcg
    13501 gtcgtagcaa catccgacgt ggtgatgagt gttaaaagag acactctgat gttcagaggg
    13561 gttatcgcgt ttacggatcg tggatggcag aaaactttca ggatcaggta tccattttaa
    13621 accagaaatt gagtgagttt gccccatcca tgccccaagc ggtgagattg gaagtaatta
    13681 acaacaggtt acaaaaccta cacctgcatg gacatcactt cctgatacgc cgccaccagc
    13741 ttattacgca cctgaatccc catttgcata gaaactgagg ctttttgcat atcggtcatc
    13801 acatcgttta acgccacgcc gggttcaccg agggtgaatt tttctgcctg cgtgcgggca
    13861 gctgtttgtg tatcgctaat gcgatcgagc gtggcgtgca gctgcccggc aaaactaatg
    13921 gtcggttgcg gcagtgaatc ctgcgcacgc gcactcatcg ccgtagcctg taactggctg
    13981 ataacccctt caatcccctg tatcgctgac attttcgtct cctggataat ttctggtagc
    14041 aaagcctacc agtaagtcaa taagataaag gcgctaaata acaacaaaaa aacgggttta
    14101 ttggcggata gaaaaaaacg aaagcacaaa taatggcagc gtcaattttt cgagtttgct
    14161 gacccgggag tgagtcttgt tccactttgc caataacgcc gtccataatc agccacgagg
    14221 tgcgcgatga atgcgactgc agcccagaca aaatctcttg agtggcttaa tcgcctgcgt
    14281 gcgaatccga aaattccatt gattgttgcc ggttccgcgg cagtggcggt catggtcgca
    14341 ctgatcctgt gggcgaaagc ccccgactac cgcacattat tcagcaatct ttccgatcag
    14401 gatggtggcg caattgtcag ccaactgacg caaatgaata ttccttaccg cttcagcgaa
    14461 gccagcggcg ctattgaagt tccggcagat aaagttcacg aactgcgtct gcgcctggca
    14521 caacaaggtt tgcccaaagg cggcgcggtc ggtttcgaac tactcgatca ggaaaagttt
    14581 ggtatcagcc agttcagcga acaggtgaat tatcagcggg cgctggaagg cgagctttct
    14641 cgtaccatcg aaactatcgg cccggtaaaa ggggcgcgcg tacatctggc aatgccgaaa
    14701 ccgtctttat tcgtccgtga acaaaaatcc ccttctgcat cggtgacggt aaatctgtta
    14761 cccggccgcg cactcgatga agggcaaatt agcgccattg tgcatctggt ttccagcgcc
    14821 gttgctggtc tgccgccggg aaacgtcacg ctggtggatc agggcggaca tctgttaacc
    14881 cagtccaata ccagcgggcg cgatcttaat gatgctcagt tgaaatatgc cagcgatgtc
    14941 gaaggccgta ttcagcggcg tattgaagca atcctgtcgc ctattgttgg taacggtaat
    15001 attcacgccc aggttacggc gcagctggac ttcgccagta aagaacaaac ggaagaacag
    15061 tatcgcccta acggtgatga atctcatgcg gcgcttcgtt cacgccagct taatgagagc
    15121 gagcaaagcg gttccggtta tccgggcggc gtaccggggg cgttgtcgaa tcaaccggca
    15181 cctgcgaata acgcgccaat cagcacgcct ccggcaaatc aaaataaccg ccagcagcag
    15241 gcgagcacca ccagcaatag cgggccgcgt agcacacagc ggaatgaaac cagtaactac
    15301 gaagtcgatc gcaccattcg tcataccaaa atgaacgtgg gcgatgtgca acgtctgtca
    15361 gtcgcggtag tggtgaatta caaaaccttg ccagacggta aaccattgcc tctcagcaac
    15421 gaacagatga agcaaattga agatctgacc cgcgaggcga tgggcttttc tgaaaaacgc
    15481 ggtgactcgc tcaatgtcgt taactcgccg ttcaatagca gtgacgaaag cggcggagaa
    15541 ctgccgttct ggcaacagca agcgtttatc gatcagttgc tggctgccgg tcgctggttg
    15601 ctggtactgc tggtggcgtg gctgctgtgg cggaaagcgg tacgcccgca gttaacacgt
    15661 cgcgccgagg cgatgaaagc tgtacagcaa caggcgcagg cccgtgagga agtggaagat
    15721 gcggtggaag tccgcctgag caaagacgaa caactccaac aacgacgcgc taaccaacgt
    15781 ctgggggcag aagtcatgag ccagcgtatc cgtgaaatgt ctgataacga tccgcgcgtg
    15841 gtggcgctgg tcattcgcca gtggataaat aacgatcatg agtaacctga caggcaccga
    15901 taaaagcgtc atcctgctga tgaccattgg cgaagaccgg gcggcagagg tgttcaagca
    15961 cctctcccag cgcgaagtgc aaaccctgag cgctgcaatg gcgaacgtca cgcagatctc
    16021 caacaagcag ctaaccgatg tgctggcgga gtttgagcaa gaagctgaac agtttgccgc
    16081 actgaatatc aacgccaacg attatctgcg ttcggtattg gtcaaagctc tgggtgagga
    16141 acgtgccgcc agcctgctgg aagatattct cgaaactcgc gataccgcca gcggtattga
    16201 aacgctcaac tttatggagc cgcagagtgc cgccgatctg attcgcgatg agcatccgca
    16261 aattatcgcc accattctgg tgcatctgaa gcgcgcccag gccgccgata ttctggcgtt
    16321 gttcgatgaa cgtctgcgcc acgacgtgat gttgcgtatc gccacctttg gcggcgtgca
    16381 gccagccgcg ctggcggaac tgaccgaagt actgaatggc ttgctcgacg gtcagaatct
    16441 caagcgcagc aaaatgggcg gcgtgagaac ggcagccgaa attatcaacc tgatgaaaac
    16501 tcagcaggaa gaagccgtta ttaccgccgt gcgcgaattc gacggcgagc tggcgcaaaa
    16561 aattatcgac gagatgttcc tgttcgaaaa tctggtggat gtcgacgatc gcagcattca
    16621 gcgtctgttg caggaagtgg attccgaatc gctgttgatc gcactgaaag gggccgagca
    16681 gccactgcgc gagaaattcc tgcgcaatat gtcgcagcgt gccgccgata ttctgcgcga
    16741 cgatctcgcc aaccgtggtc cggtgcgtct gtcgcaggtg gaaaacgaac agaaagcgat
    16801 tctgctgatt gtgcgccgcc ttgccgaaac tggcgagatg gtaattggca gcggcgagga
    16861 tacctatgtc tgataatctg ccgtggaaaa cctggacgcc ggacgatctc gcgccaccac
    16921 aggcagagtt tgtgcccatg gtcgagccgg aagaaaccat cattgaagag gccgaaccca
    16981 gccttgagca gcaactggcg caactgcaaa tgcaggccca tgagcaaggt tatcaggcgg
    17041 gtattgccga aggtcgccag caaggtcata agcagggcta tcaggaagga ctggcccagg
    17101 ggctggagca aggtctggca gaggcgaagt ctcaacaagc gccaattcat gcccggatgc
    17161 agcaactggt cagcgaattt caaactaccc ttgatgcact tgatagtgtg attgcttcgc
    17221 gcctgatgca gatggcgctg gaggcggcac gtcaggtcat cggtcagacg ccaacagtgg
    17281 ataactcggc actgatcaaa cagatccaac agttgttgca gcaagaaccg ttattcagcg
    17341 gcaaaccgca actacgcgtg caccccgatg atctgcaacg tgtggatgat atgctcggcg
    17401 caaccttaag tttgcatggc tggcgcttgc ggggcgatcc cacactccat cctggcggct
    17461 gtaaagtctc cgccgatgaa ggcgatctcg acgccagtgt cgccactcgc tggcaagaac
    17521 tctgccgtct ggcagcacca ggagtggtgt aatgaccacg cgcctgactc gctggctaac
    17581 cacgctggat aactttgaag ccaaaatggc gcagttgcct gcggtacgtc gctacgggcg
    17641 attaactcgc gctaccgggc tggtgctgga agccaccgga ttacaattgc cgctcggcgc
    17701 aacctgtgtc attgagcgcc agaacggcag cgaaacgcac gaagtagaaa gcgaagtcgt
    17761 tggctttaac ggtcaacggc tgtttttaat gccgctggag gaagtcgaag gtgtcctgcc
    17821 cggcgcgcgt gtttatgcca aaaacatttc ggcagaaggg ctgcaaagcg gcaagcagtt
    17881 gccgctcggt ccggcgttat taggtcgtgt tctggacggc agcggtaaac cgctcgatgg
    17941 cctcccctcc cccgatacga cggaaaccgg tgcgctgatt accccaccat ttaacccgtt
    18001 gcaacgtaca ccgattgaac atgtgctgga caccggcgtg cgcccaatca atgccctgct
    18061 taccgttggg cgtgggcagc gtatggggct gtttgccggg tccggcgttg gtaaaagtgt
    18121 gctgctgggg atgatggcac gttacacccg cgccgatgtc attgtcgtgg gtttgattgg
    18181 tgaacgcgga cgcgaagtaa aagattttat tgagaatatc ctcggtgccg aagggcgtgc
    18241 acgctccgtg gtgattgccg ctccggcgga tgtctctccg cttctgcgaa tgcaaggtgc
    18301 cgcctatgcc acgcgaattg ccgaagattt tcgcgatcgt ggtcagcatg tgttgctgat
    18361 tatggactcc ctcacccgct acgcgatggc ccagcgtgaa attgcgctgg cgattggcga
    18421 accacctgcc actaaaggtt atccaccgtc ggtgtttgcc aaattaccgg cactggtcga
    18481 gcgtgccgga aatggaatta gcggcggcgg ctcgattacc gcgttttata ccgtgctcac
    18541 cgaaggcgat gaccagcagg accccattgc cgactctgcg cgggcaatcc tcgacggtca
    18601 cattgtgctg tctcgccgac tggcggaagc cgggcactat ccggctatcg atattgaagc
    18661 gtcgatcagc cgcgcaatga cggcgttgat cagtgagcaa cattacgcgc gagtgcgcac
    18721 cttcaaacag ctgttgtcga gttttcagcg taaccgcgat ctggttagcg tcggcgcgta
    18781 tgccaaaggc agcgatccga tgctcgacaa agccatcgcc ctgtggccgc agctggaggg
    18841 ctatttgcaa caaggcattt ttgaacgcgc ggactgggaa gcgtctctcc aggggctgga
    18901 gcgtattttc ccgacagtgt cataacccag gagataacgg cagatggcag aacatggcgc
    18961 gctggcgacc ctgaaagatc tggcagaaaa agaggtagag gatgccgcgc gcctgctggg
    19021 tgaaatgcgt cgcgggtgtc agcaggcgga agaacagctc aaaatgctga tcgattatca
    19081 gaatgaatat cgcaataacc tcaacagcga tatgagtgcc gggatgacca gcaaccgctg
    19141 gatcaactat cagcagttta tccagacgct ggaaaaagcc attactcagc atcgccagca
    19201 acttaatcag tggacgcaga aagttgacat tgccctgaac agctggcgag aaaagaaaca
    19261 acgtttgcag gcctggcaga cactgcagga acggcaatcc acggcggcac tgcttgcaga
    19321 aaaccgcctc gatcagaaaa agatggatga gttcgcccag cgcgccgcca tgaggaaacc
    19381 tgaatgattc gcttagcgcc cttaattacc gccgacgttg ataacgccac attgcctggc
    19441 ggcaaagcca gcgatgctgc acaagatttt ctcgcgttgt tgagcgaagc attagcaggc
    19501 gagacaacta ccgacaaagc ggccccccag ttgctggtgg caacagataa gcccacgaca
    19561 aaaggcgagc cgctggtcag cgagattctt gccgatgcac aacaagcgga tttactgatc
    19621 cctgtggatg aaacaccgcc tgtcatcaac gacgaacaat ccacatcaac accattaacc
    19681 accgctcaaa cgatgacgtt ggctgcggtg gctgacaaca atacggcaaa agacgaaaaa
    19741 gcggatgatc tgaatgaaga cgtcaccgca agcctgagcg ccctttttgc gatgttgccg
    19801 ggttttgaca atacgcccaa agtgactgat gtgccgtcaa ccgtgttacc ggcagagaaa
    19861 ccaacgctct tcacaaaact gacttctgcg caactcacaa cagcacagcc tgatgatgcc
    19921 cccggcacgc cagctcagcc attaacaccg ctggtagcag aagcccagag taaagcggaa
    19981 gtcatcagca caccttcacc ggtgaccgct gccgccagcc cgctaatcac tccacaccag
    20041 acacagccac tgcccaccgt cgccgtgcct gtgttgagtg caccgctggg ttctcacgaa
    20101 tggcaacaat cattaagcca gcatatttcg ctgttcaccc gccagaggca acaaagtgca
    20161 gagttgcgtc tgcacccgca ggatttaggt gaagtgcaaa tctccctcaa agtggatgat
    20221 aaccaggcac aaatccagat ggtttcaccg catcagcatg tacgcgccgc gctggaagca
    20281 gcgcttccgg tactgcgtac gcagctggcc gaaagtggca ttcagttagg gcaaagcaac
    20341 atcagtggcg aaagctttag tggtcagcag caggccgctt cccaacaaca gcaaagccaa
    20401 cgcacagtaa accatgaacc tctggcgggg gaagacgacg atacgcttct ggttcccgtc
    20461 tctatacaag ggcgcgtaac aggcaacagc ggcgttgata ttttcgccta acgtcagagg
    20521 tagcaccgta atccgcgtct tttccccgct ttgttgcgct caagacgcag gataattagc
    20581 cgataagcag tagcgacaca ggaagaccgc aacacatgac tgattacgcg ataagcaaga
    20641 aaagcaagcg atcgctttgg atcccgattc tggtattcat taccctcgcg gcctgtgcca
    20701 gcgcaggtta cagctactgg cattcgcatc aggttgccgc tgacgacaaa gcgcagcaac
    20761 gcgtcgtacc ctccccggtc ttctacgcgc tggatacctt cacggtcaat ctgggcgatg
    20821 cggatcgcgt gctttatatc ggcataaccc tgcgcctgaa agatgaagct acccgctcgc
    20881 ggctgagtga gtatttgccg gaagtccgta gccgtttgct gttactgttt tcgcgtcagg
    20941 atgctgccgt actggcgaca gaagaaggca agaaaaacct gattgccgag attaaaacca
    21001 cgctttccac cccgcttgtt gccgggcaac cgaaacagga tgtcaccgac gtgctgtata
    21061 ccgcttttat tctgcgataa cgacatgggc gatagtattc tttctcaagc tgaaattgat
    21121 gcgctgttga atggtgacag cgaagtcaaa gacgaaccga cagccagtgt tagcggcgaa
    21181 agtgacattc gtccgtacga tccgaatacc caacgacggg ttgtgcgcga acgtttgcag
    21241 gcgctggaaa tcattaatga gcgctttgcc cgccattttc gtatggggct gttcaacctg
    21301 ctgcgtcgta gcccggatat aaccgtcggg gccatccgca ttcagccgta ccatgaattt
    21361 gcccgcaacc tgccggtgcc gaccaacctg aaccttatcc atctgaaacc gctgcgcggc
    21421 acagggctgg tggtgttttc accgagtctg gtgtttatcg ccgtggataa cctgtttggc
    21481 ggcgatggac gcttcccgac caaagtggaa ggtcgcgagt ttacccatac cgaacagcgc
    21541 gtcatcaacc gcatgttaaa gctggcgctt gaaggctata gcgacgcctg gaaggcgatt
    21601 aatccgctgg aagttgagta cgtgcgttcg gaaatgcagg tgaaatttac caatatcacc
    21661 acctcgccga acgacattgt ggttaacacg ccgttccatg tggagattgg caacctgacc
    21721 ggcgaattta atatctgcct gcctttcagc atgatcgagc cgctacggga attgttggtt
    21781 aacccgccgc tggaaaactc gcgtaatgaa gatcagaact ggcgcgataa cctggtgcgc
    21841 caggtgcagc attcacagct ggagctggtc gccaactttg ccgatatctc gctacgcctg
    21901 tcgcagattt taaaactgaa acccggtgac gtcctgccga tagaaaaacc cgaccgcatc
    21961 atcgcccatg ttgacggcgt cccggtgctg accagtcagt acggcactct caacggtcag
    22021 tatgcgttac ggatagaaca tttgattaac ccgattttaa attctctgaa cgaggaacag
    22081 cccaaatgag tgacatgaat aatccggccg atgacaacaa cggcgcaatg gacgatctgt
    22141 gggctgaagc tttgagcgaa caaaaatcaa ccagcagcaa aagcgctgcc gacgcggtgt
    22201 tccagcaatt tggcggtggt gatgtcagcg gaacgttgca ggatatcgac ctgattatgg
    22261 atattccggt caagctgacc gtcgagctgg gccgtacgcg gatgaccatc aaagagctgt
    22321 tgcgtctgac gcaagggtcc gtcgtggcgc tggacggtct ggcgggcgaa ccactggata
    22381 ttctgatcaa cggttattta attgcccagg gcgaagtggt ggtcgttgcc gataaatatg
    22441 gcgtgcggat caccgatatc attactccgt ctgagcgaat gcgccgcctg agccgttagt
    22501 gatgaataac cacgctactg tgcaatcttc cgcgccggtt tctgctgcgc cactgctgca
    22561 ggtgagcggc gcactcatcg ccattattgc cctgatcctc gctgctgcct ggctggtaaa
    22621 acggttggga tttgtcccta aacgcactgg cgttaacggt ctgaaaatta gcgccagtgc
    22681 ttcgctgggc gcgcgtgaac gggttgtggt ggtcgatgtg gaagatgcac ggctggtgct
    22741 cggcgttacc gcagggcaaa tcaatctgct gcataaactt cccccttcag caccaacgga
    22801 agagataccg cagcccgatt ttcagtcggt tatgaaaaac ttgcttaagc gtagcgggag
    22861 atcctgatgc gtcgtttgtt gtctgtcgca cctgtccttc tctggctggt tacgcccctc
    22921 gccttcgcgc aactgccggg tatcaccagc cagccgctgc ctggcggtgg acaaagctgg
    22981 tcgctcccgg tgcaaacgct ggtgttcatc acctcgttga cgtttattcc ggcaatttta
    23041 ctgatgatga ccagtttcac ccgcatcatc attgtttttg gtttattgcg taacgcgttg
    23101 ggaacaccct ccgcgccacc taaccaggta ttgctgggac tggcgctgtt tttgaccttt
    23161 tttattatgt cgccggtgat cgataaaatt tatgtcgatg cgtaccagcc attcagcgaa
    23221 gagaaaatct caatgcagga ggcgctggaa aaaggggcgc agccgctgcg tgagtttatg
    23281 ctgcgtcaga cccgcgaggc ggatttaggg ttgtttgcca gactggcgaa tactggcccg
    23341 ttgcagggac ctgaagctgt gccgatgcgc attttgctcc cggcctacgt gaccagcgag
    23401 ttgaaaaccg catttcagat aggcttcacg attttcattc cttttttgat tatcgacctg
    23461 gtgattgcca gcgtgttgat ggcattgggg atgatgatgg ttcccccagc cacaattgct
    23521 ctgcccttta aactgatgct gtttgtactg gtggatggct ggcaattgct ggtcggttcg
    23581 ctggcgcaga gcttttacag ctagagaggc aaaatgacac ctgaatcggt catgatgatg
    23641 gggactgaag cgatgaaagt cgcgctggca ctggctgccc cactgttgct ggtggcgctg
    23701 gtcacgggtc ttatcatcag tattttgcag gccgccaccc agattaacga aatgacgctg
    23761 tcgtttattc cgaaaatcat cgccgtattt atcgccatta ttattgccgg accgtggatg
    23821 ctcaatctgt tgctggatta cgtccgcacc ttgttcacta acctgccgta tatcatcggg
    23881 taggccgtac aatgatgcag gtaacaagcg atcaatggct ttcctggtta agcctgtact
    23941 tctggccgtt actgcgcgtg ctggcgctca tttctaccgc accaattctg agcgaacgca
    24001 gcgtgccgaa acgggtaaaa ctgggtctgg caatgatgat cacgttcgct attgccccat
    24061 cattacctgc caacgatgtt cctgtttttt cgttctttgc tctgtggctg gccgtgcagc
    24121 agatcctgat cggcattgcg cttggtttta ccatgcaatt tgcctttgcc gctgtgcgaa
    24181 ccgctggtga aattatcggt ctgcaaatgg ggctgtcatt tgcgacgttt gtcgatccgg
    24241 ccagccatct caatatgccc gttttagcgc gtatcatgga tatgctggcg ttactgctgt
    24301 tcctgacatt taacggtcat ttatggttga tttcactgct ggccgatacc tttcacaccc
    24361 tgccaattgg tggcgaaccg ttgaacagca atgcgtttct ggcactcacc aaagcaggga
    24421 gtttgatttt ccttaacggg ctgatgctgg cattaccgct cattactctg ctgctgaccc
    24481 tgaatctggc attaggctta cttaatcgta tggccccgca attatccatt tttgttattg
    24541 gatttccatt aattctgact gtcggtatct ctttaatggc ggcattaatg ccgttaattg
    24601 cgcctttttg cgaacattta ttcagtgaga tgtttaattt gctggctgat attattagtg
    24661 aattgccatt aatataattc cgtaacgttt atcatgttat cctaaggatt atccgaaaaa
    24721 taatacctac gaacatcttc caggatactc ctgcggcgaa atatttgttt taagctcact
    24781 cacatatcgc aacatttact ttactttaag acaattccag gcaaattata caacacttta
    24841 cgggatagta agtccgcctg aaaaatcgcg agagtggcgc attaggtgac ccatgttgtt
    24901 ccgtttagtc atgatgaaat attcaggtaa ggggaattat cgttacgcat tgagtgaggg
    24961 tatgccatgt caacgattat tatggattta tgtagttaca cccgactagg tttaaccggg
    25021 tatctgttga gtagaggggt taaaaaaaga gaaatcaacg acattgaaac cgttgatgac
    25081 cttgccatag cttgtgattc acagcgccct tcagtggtgt ttattaatga ggactgtttc
    25141 atccacgatg cttctaacag tcagcgtatc aagctcat
//
LOCUS       DAZKHF020000044        25154 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2201S31241
            SAMN43267800-rid25106413.denovo.044, whole genome shotgun sequence.
ACCESSION   DAZKHF020000044 DAZKHF020000000
VERSION     DAZKHF020000044.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267800
            Sequence Read Archive: SRR30307263
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 25154)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25154)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 25154)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350581.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350581.2
            Genome Coverage       :: 151x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:21:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,652
            CDSs (total)                      :: 5,557
            Genes (coding)                    :: 5,320
            CDSs (with protein)               :: 5,320
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 237
            CDSs (without protein)            :: 237
            Pseudo Genes (ambiguous residues) :: 0 of 237
            Pseudo Genes (frameshifted)       :: 102 of 237
            Pseudo Genes (incomplete)         :: 130 of 237
            Pseudo Genes (internal stop)      :: 52 of 237
            Pseudo Genes (multiple problems)  :: 41 of 237
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25154
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267800-rid25106413.denovo.044"
                     /serotype="O 26:H11"
                     /isolate="2201S31241"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-01"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            72..803
                     /gene="artJ"
                     /locus_tag="ACHOSC_RS07280"
                     /old_locus_tag="pgaptmp_001456"
     CDS             72..803
                     /gene="artJ"
                     /locus_tag="ACHOSC_RS07280"
                     /old_locus_tag="pgaptmp_001456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine ABC transporter substrate-binding
                     protein ArtJ"
                     /protein_id="HGU4547430.1"
                     /translation="MKKLVLAALLASFTFGASAAEKINFGVSATYPPFESIGANNEMV
                     GFDIDLAKALCKQMQAECTFTNHAFDSLIPSLKFRKYDAVISGMDITPERSKQVSFTT
                     PYYENSAVVIAKKDTYKTFADLKGKRIGMENGTTHQKYIQDQHPEVKTVSYDSYQNAF
                     IDLKNGRIDGVFGDTAVVNEWLKTNPQLGVATEKVTDPQYFGTGLGIAVRPDNKALLE
                     KLNNALAAIKADGTYQKINDQWFPQ"
     gene            complement(978..2105)
                     /gene="rlmC"
                     /locus_tag="ACHOSC_RS07285"
                     /old_locus_tag="pgaptmp_001457"
     CDS             complement(978..2105)
                     /gene="rlmC"
                     /locus_tag="ACHOSC_RS07285"
                     /old_locus_tag="pgaptmp_001457"
                     /EC_number="2.1.1.189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008649 - rRNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0016070 - RNA metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uracil(747)-C(5))-methyltransferase
                     RlmC"
                     /protein_id="HGU4547431.1"
                     /translation="MQCALYDAGRCRSCQWITQPIPEQLSAKTADLKNLLADFPVEEW
                     CAPVSGPEQGFRNKAKMVVSGSVEKPLLGMLHRDGTPEDLCDCPLYPASFAPVFAALK
                     PFIARAGLTPYNVARKRGELKYILLTESQSDGGMMLRFVLRSETKLAQLRKALPWLQE
                     QLPQLKVITVNIQPVHMAIMEGETEIYLTEQQALAERFNDVPLWIRPQSFFQTNPAVA
                     SQLYATARDWVRQLPVKHMWDLFCGVGGFGLHCATPDMQLTGIEIASEAIACAKQSAA
                     ELGLTRLQFQALDSTQFATAQGEVPELVLVNPPRRGIGKPLCDYLSTMAPRFIIYSSC
                     NAQTMAKDIRELPGYRIERVQLFDMFPHTAHYEVLTLLVKQ"
     gene            complement(2146..2634)
                     /locus_tag="ACHOSC_RS07290"
                     /old_locus_tag="pgaptmp_001458"
     CDS             complement(2146..2634)
                     /locus_tag="ACHOSC_RS07290"
                     /old_locus_tag="pgaptmp_001458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbjO family protein"
                     /protein_id="HGU4547432.1"
                     /translation="MEDETLGFFKKTSSSHARLNVPALVQVAALAIIMIRGLDVLMIF
                     NTLGVRGIGEFIHRSVQTWSLTLVFLSSLVLVFIEIWCAFSLVKGRRWARWLYLLTQI
                     TAASYLWAASLGYGYPELFSIPGESKREIFHSLMLQKLPDMLILMLLFVPSTSRRFFQ
                     LQ"
     gene            complement(2694..3539)
                     /gene="potI"
                     /locus_tag="ACHOSC_RS07295"
                     /old_locus_tag="pgaptmp_001459"
     CDS             complement(2694..3539)
                     /gene="potI"
                     /locus_tag="ACHOSC_RS07295"
                     /old_locus_tag="pgaptmp_001459"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putrescine ABC transporter permease PotI"
                     /protein_id="HGU4547433.1"
                     /translation="MNNLPVVRSPWRIVILLLGFTFLYAPMLMLVIYSFNSSKLVTVW
                     AGWSTRWYGELLRDDAMMSAVGLSLTIAACAATAAAILGTIAAVVLVRFGRFRGSNGF
                     AFMITAPLVMPDVITGLSLLLLFVALAHAIGWPADRGMLTIWLAHVTFCTAYVAVVIS
                     SRLRELDRSIEEAAMDLGATPLKVFFVITLPMIMPAIISGWLLAFTLSLDDLVIASFV
                     SGPGATTLPMLVFSSVRMGVNPEINALATLILGAVGIVGFIAWYLMARAEKQRIRDIQ
                     RARHG"
     gene            complement(3536..4489)
                     /gene="potH"
                     /locus_tag="ACHOSC_RS07300"
                     /old_locus_tag="pgaptmp_001460"
     CDS             complement(3536..4489)
                     /gene="potH"
                     /locus_tag="ACHOSC_RS07300"
                     /old_locus_tag="pgaptmp_001460"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putrescine ABC transporter permease PotH"
                     /protein_id="HGU4547434.1"
                     /translation="MNTLEPAAQSKPPGGFKLWLSQLQMKHGRKLVIALPYIWLILLF
                     LLPFLIVFKISLAEMARAIPPYTELMEWADGQLSITLNLGNFLQLTDDPLYFDAYLQS
                     LQVAAISTFCCLLIGYPLAWAVAHSKPSTRNILLLLVILPSWTSFLIRVYAWMGILKN
                     NGVLNNFLLWLGVIDQPLTILHTNLAVYIGIVYAYVPFMVLPIYTALIRIDYSLVEAA
                     LDLGARPLKTFFTVIVPLTKGGIIAGSMLVFIPAVGEFVIPELLGGPDSIMIGRVLWQ
                     EFFNNRDWPVASAVAIIMLLLLIVPIMWFHKHQQKSVGEHG"
     gene            complement(4499..5632)
                     /gene="potG"
                     /locus_tag="ACHOSC_RS07305"
                     /old_locus_tag="pgaptmp_001461"
     CDS             complement(4499..5632)
                     /gene="potG"
                     /locus_tag="ACHOSC_RS07305"
                     /old_locus_tag="pgaptmp_001461"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000996010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015417 - ABC-type polyamine transporter
                     activity [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015846 - polyamine transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putrescine ABC transporter ATP-binding subunit
                     PotG"
                     /protein_id="HGU4547435.1"
                     /translation="MNDAIPRPQAKTRKALTPLLEIRNLTKSYDGQHAVDDVSLTIYK
                     GEIFALLGASGCGKSTLLRMLAGFEQPSAGQIMLDGVDLSQVPPYLRPINMMFQSYAL
                     FPHMTVEQNIAFGLKQDKLPKAEIATRVNEMLGLVHMQEFAKRKPHQLSGGQRQRVAL
                     ARSLAKRPKLLLLDEPMGALDKKLRDRMQLEVVDILERVGVTCVMVTHDQEEAMTMAG
                     RIAIMNRGKFVQIGEPEEIYEHPTTRYSAEFIGSVNVFEGVLKERQEDGLVLDSPGLV
                     HPLKVDADASVVDNVPVHVALRPEKIMLCEEPPANGCNFAVGEVIHIAYLGDLSVYHV
                     RLKSGQMISAQLQNAHRHRKGLPTWGDEVRLCWEVDSCVVLTV"
     gene            complement(5727..6839)
                     /gene="potF"
                     /locus_tag="ACHOSC_RS07310"
                     /old_locus_tag="pgaptmp_001462"
     CDS             complement(5727..6839)
                     /gene="potF"
                     /locus_tag="ACHOSC_RS07310"
                     /old_locus_tag="pgaptmp_001462"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA];
                     GO_function: GO:0019808 - polyamine binding [Evidence
                     IEA];
                     GO_process: GO:0015846 - polyamine transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="spermidine/putrescine ABC transporter
                     substrate-binding protein PotF"
                     /protein_id="HGU4547436.1"
                     /translation="MTALNKKWLSGLAAGALMAVSVGTLAAEQKTLHVYNWSDYIAPD
                     TVANFEKETGIKVVYDVFDSNEVLEGKLMAGSTGFDLVVPSASFLERQLTAGVFQPLD
                     KSKLPEWKNLDPELLKLVAKHDPDNKFAMPYMWATTGIGYNVDKVKAVLGENAPVDSW
                     DLILKPENLEKLKSCGVSFLDAPEEVFATVLNYLGKDPNSTKADDYTGPATDLLLKLR
                     PNIRYFHSSQYINDLANGDICVAIGWAGDVWQASNRAKEAKNGVNVSFSIPKEGAMAF
                     FDVFAMPADAKNKDEAYQFLNYLLRPDVVAHISDNVFYANANKAATPLVSAEVRENPG
                     IYPPADVRAKLFTLKVQDPKIDRVRTRAWTKVKSGK"
     gene            complement(7190..7666)
                     /locus_tag="ACHOSC_RS07315"
                     /old_locus_tag="pgaptmp_001463"
     CDS             complement(7190..7666)
                     /locus_tag="ACHOSC_RS07315"
                     /old_locus_tag="pgaptmp_001463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbjN domain-containing protein"
                     /protein_id="HGU4547437.1"
                     /translation="MTSLVVPGLDTLRQWLDDLGMSFFECDNCQALHLPHMQNFDGVF
                     DAKIDLIDNTILFSAMAEVRPSAVLPLAADLSAINASSLTVKAFLDMQDDNLPKLVVC
                     QSLSVMQGVTYEQFAWFVRQSEEQISMVILEANAHQLLLPTDDEGQNNVTENYFLH"
     gene            complement(7754..8656)
                     /gene="rimK"
                     /locus_tag="ACHOSC_RS07320"
                     /old_locus_tag="pgaptmp_001464"
     CDS             complement(7754..8656)
                     /gene="rimK"
                     /locus_tag="ACHOSC_RS07320"
                     /old_locus_tag="pgaptmp_001464"
                     /EC_number="6.3.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S6--L-glutamate ligase"
                     /protein_id="HGU4547438.1"
                     /translation="MKIAILSRDGTLYSCKRLREAAIQRGHLVEILDPLSCYMNINPA
                     ASSIHYKGRKLPHFDAVIPRIGTAITFYGTAALRQFEMLGSYPLNESVAIARARDKLR
                     SMQLLARQGIDLPVTGIAHSPDDTSDLIDMVGGAPLVVKLVEGTQGIGVVLAETRQAA
                     ESVIDAFRGLNAHILVQEYIKEAQGCDIRCLVVGDEVVAAIERRAKEGDFRSNLHRGG
                     AASVASITPQEREIAIKAARTMALDVAGVDILRANRGPLVMEVNASPGLEGIEKTTGI
                     DIAGKMIRWIERHATTEYCLKTGG"
     gene            complement(8717..9439)
                     /gene="nfsA"
                     /locus_tag="ACHOSC_RS07325"
                     /old_locus_tag="pgaptmp_001465"
     CDS             complement(8717..9439)
                     /gene="nfsA"
                     /locus_tag="ACHOSC_RS07325"
                     /old_locus_tag="pgaptmp_001465"
                     /EC_number="1.5.1.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase NfsA"
                     /protein_id="HGU4547439.1"
                     /translation="MTPTIELICGHRSIRHFTDEPISEAQREAIINSARATSSSSFLQ
                     CSSIIRITDKALREELVTLTGGQKHVAQAAEFWVFCADFNRHLQICPDAQLGLAEQLL
                     LGVVDTAMMAQNALIAAESLGLGGVYIGGLRNNIEAVTKLLKLPQHVLPLFGLCLGWP
                     ADNPDLKPRLPASILVHENSYQPLDKGALAQYDEQLAEYYLTRGSNNRRDTWSDHIRR
                     TIIKESRPFILDYLHKQGWATR"
     gene            complement(9423..9710)
                     /locus_tag="ACHOSC_RS07330"
                     /old_locus_tag="pgaptmp_001466"
     CDS             complement(9423..9710)
                     /locus_tag="ACHOSC_RS07330"
                     /old_locus_tag="pgaptmp_001466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1418 family protein"
                     /protein_id="HGU4547440.1"
                     /translation="MRAIGKLPKGVLILEFIGMMLLAVALLSVSDSLSLPEPFSRPEV
                     QILMIFLGVLLMLPAAVVVILQVAKRLAPQLMNRPPQYSRSEREKDNDANH"
     gene            9870..10127
                     /gene="grxA"
                     /locus_tag="ACHOSC_RS07335"
                     /old_locus_tag="pgaptmp_001467"
     CDS             9870..10127
                     /gene="grxA"
                     /locus_tag="ACHOSC_RS07335"
                     /old_locus_tag="pgaptmp_001467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin 1"
                     /protein_id="HGU4547441.1"
                     /translation="MQTVIFGRSGCPYCVRAKDLAEKLSNERDDFQYQYVDIRAEGIT
                     KEDLQQKAGKPVETVPQIFVDQQHIGGYTDFAAWVKENLDA"
     gene            complement(10157..10534)
                     /locus_tag="ACHOSC_RS07340"
                     /old_locus_tag="pgaptmp_001468"
     CDS             complement(10157..10534)
                     /locus_tag="ACHOSC_RS07340"
                     /old_locus_tag="pgaptmp_001468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inner membrane protein YbjM"
                     /protein_id="HGU4547442.1"
                     /translation="MKHKQRWAGAICCFVLFIVVCLFLATHMKGAFRAAGHPEIGLLF
                     FILPGAVASFFSQRREVLKPLFGAMLAAPCSMLIMRLFFSPTRSFWQELAWLLSAVFW
                     CALGALCFLFISSLFKPQHRKNQ"
     gene            10804..12489
                     /locus_tag="ACHOSC_RS07345"
                     /old_locus_tag="pgaptmp_001469"
     CDS             10804..12489
                     /locus_tag="ACHOSC_RS07345"
                     /old_locus_tag="pgaptmp_001469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001024888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate:alanine antiporter"
                     /protein_id="HGU4547443.1"
                     /translation="MNINVAELLNGNYILLLFVVLALGLCLGKLRLGSIQLGNSIGVL
                     VVSLLLGQQHFSINTDALNLGFMLFIFCVGVEAGPNFFSIFFRDGKNYLMLALVMVGS
                     ALVIALGLGKLFGWDIGLTAGMLAGSMTSTPVLVGAGDTLRHSGMESRQLSLALDNLS
                     LGYALTYLIGLVSLIVGARYLPKLQHQDLQTSAQQIARERGLDTDANRKVYLPVIRAY
                     RVGPELVAWTDGKNLRELGIYRQTGCYIERIRRNGILANPDGDAVLQMGDEIALVGYP
                     DAHARLDPSFRNGKEVFDRDLLDMRIVTEEVVVKNHNAVGKRLAQLKLTDHGCFLNRV
                     IRSQIEMPIDDNVVLNKGDVLQVSGDARRVKTIADRIGFISIHSQVTDLLAFCAFFVI
                     GLMIGMITFQFSTFSFGMGNAAGLLFAGIMLGFMRANHPTFGYIPQGALSMVKEFGLM
                     VFMAGVGLSAGSGINNGLGAIGGQMLIAGLIVSLVPVVICFLFGAYVLRMNRALLFGA
                     MMGARTCAPAMEIISDTARSNIPALGYAGTYAIANVLLTLAGTIIVMVWPGLG"
     gene            complement(12664..13200)
                     /gene="rcdA"
                     /locus_tag="ACHOSC_RS07350"
                     /old_locus_tag="pgaptmp_001470"
     CDS             complement(12664..13200)
                     /gene="rcdA"
                     /locus_tag="ACHOSC_RS07350"
                     /old_locus_tag="pgaptmp_001470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator RcdA"
                     /protein_id="HGU4547444.1"
                     /translation="MRRANDPQRREKIIQATLEAVKLYGIHAVTHRKIATLAGVPLGS
                     MTYYFSGIDELLLEAFSSFTEIMSRQYQAFFSDVSDAQGACQAITDMIYSSQVATPDN
                     MELMYQLYALASRKPLLKTVMQNWMQRSQQTLEQWFEPGTARALDAFIEGMTLHFVTD
                     RKPLSREEILRMVERVAG"
     gene            13284..14492
                     /locus_tag="ACHOSC_RS07355"
                     /old_locus_tag="pgaptmp_001471"
     CDS             13284..14492
                     /locus_tag="ACHOSC_RS07355"
                     /old_locus_tag="pgaptmp_001471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HGU4547445.1"
                     /translation="MTVNSSRNALKRRTWALFMFFFLPGLLMASWATRTPAIRDILSV
                     SIAEMGGVLFGLSIGSMSGILCSAWLVKRFGTRNVILVTMSCALIGMMILSLALWLTS
                     PLLFAVGLGVFGASFGSAEVAINVEGAAVEREMNKTVLPMMHGFYSLGTLAGAGVGMA
                     LTAFGVPATVHILLAALVGIAPIYIAIQAIPDGTGKNAADGTQHGEKGVPFYRDIQLL
                     LIGVVVLAMAFAEGSANDWLPLLMVDGHGFSPTSGSLIYAGFTLGMTVGRFTGGWFID
                     RYSRVAVVRASALMGALGIGLIIFVDSAWVAGVSVVLWGLGASLGFPLTISAASDTGP
                     DAPTRVSVVATTGYLAFLVGPPLLGYLGEHYGLRSAMLVVLALVILAAIVAKAVAKPD
                     TKTQTAMENS"
     gene            14492..15307
                     /gene="ybjI"
                     /locus_tag="ACHOSC_RS07360"
                     /old_locus_tag="pgaptmp_001472"
     CDS             14492..15307
                     /gene="ybjI"
                     /locus_tag="ACHOSC_RS07360"
                     /old_locus_tag="pgaptmp_001472"
                     /EC_number="3.1.3.104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415365.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-amino-6-(5-phospho-D-ribitylamino)uracil
                     phosphatase"
                     /protein_id="HGU4547446.1"
                     /translation="MSIKLIAVDMDGTFLSDQKTYNRERFMAQYQQMKAQGIRFVVAS
                     GNQYYQLISFFPEIANEIAFVAENGGWVVSEGKDVFNGELSKDAFATVVEHLLTRPEV
                     EIIACGKNSAYTLKKYDDAMKTVAEMYYHRLEYVDNFDNLEDIFFKFGLNLSDELIPQ
                     VQKALHEAIGDIMVPVHTGNGSIDLIIPGVHKANGLRQLQKLWGIDDSEVVVFGDGGN
                     DIEMLRQAGFSFAMENAGSAVVAAAKYRAGSNNREGVLDVIDKVLKHEAPFDQ"
     gene            15393..15677
                     /gene="ybjH"
                     /locus_tag="ACHOSC_RS07365"
                     /old_locus_tag="pgaptmp_001473"
     CDS             15393..15677
                     /gene="ybjH"
                     /locus_tag="ACHOSC_RS07365"
                     /old_locus_tag="pgaptmp_001473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YbjH"
                     /protein_id="HGU4547447.1"
                     /translation="MIMKNCLLLGALLMGFTGVAMAQSVTVDVPSGYKVVVVPDSVSV
                     PQAVSVATVPQTVYVAPAPAPAYRPHPYVRHLASVGEGMVIEHQIDDHHH"
     gene            complement(15718..16950)
                     /gene="mdfA"
                     /locus_tag="ACHOSC_RS07370"
                     /old_locus_tag="pgaptmp_001474"
     CDS             complement(15718..16950)
                     /gene="mdfA"
                     /locus_tag="ACHOSC_RS07370"
                     /old_locus_tag="pgaptmp_001474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter MdfA"
                     /protein_id="HGU4547448.1"
                     /translation="MQNKLASGARLGRQALLFPLCLVLYEFSTYIGNDMIQPGMLAVV
                     EQYQAGIDWVPTSMTAYLAGGMFLQWLLGPLSDRIGRRPVMLAGVVWFIITCLAILLA
                     QNIEQFTLLRFLQGISLCFIGAVGYAAIQESFEEAVCIKITALMANVALIAPLLGPLV
                     GAAWIHVLPWEGMFVLFAALAAISFFGLQQAMPETATRIGEKLSLKELGRDYKLVLKN
                     GRFVAGALALGFVSLPLLAWIAQSPIIIITGEQLSSYEYGLLQVPIFGALIAGNLLLA
                     RLTSRRTVRSLIIMGGWPIMIGLLVAAAATVISSHAYLWMTAGLSIYAFGIGLANAGL
                     VRLTLFASDMSKGTVSAAMGMLQMLIFTVGIEISKHAWLNGGNGLFNLFNLVNGILWL
                     SLMVIFLKDKQMGNSHEG"
     gene            17235..17831
                     /gene="ybjG"
                     /locus_tag="ACHOSC_RS07375"
                     /old_locus_tag="pgaptmp_001475"
     CDS             17235..17831
                     /gene="ybjG"
                     /locus_tag="ACHOSC_RS07375"
                     /old_locus_tag="pgaptmp_001475"
                     /EC_number="3.6.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0050380 - undecaprenyl-diphosphatase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-diphosphate phosphatase"
                     /protein_id="HGU4547449.1"
                     /translation="MLENLNLSLFSLINATPDSAPWMISLAIFIAKDLITVVPLLAAV
                     LWLWGLTAQRQLVIKIAIALAVSLFVSWTMGHLFPHDRPFVENIGYNFLHHAADDSFP
                     SDHGTVIFTFALAFLCWHRLWSGSLLMVLAVVIAWSRVYLGVHWPLDMLGGLLAGMIG
                     CLSAQIIWQAMGHKLYQRLQSWYRFCFALPIRKGWVRD"
     gene            17889..18647
                     /gene="deoR"
                     /locus_tag="ACHOSC_RS07380"
                     /old_locus_tag="pgaptmp_001476"
     CDS             17889..18647
                     /gene="deoR"
                     /locus_tag="ACHOSC_RS07380"
                     /old_locus_tag="pgaptmp_001476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional repressor DeoR"
                     /protein_id="HGU4547450.1"
                     /translation="METRREERIGQLLQELKRSDKLHLKDAAALLGVSEMTIRRDLNN
                     HSAPVVLLGGYIVLEPRSASHYLLSDQKSRLVEEKRRAAKMAATLVEPDQTLFFDCGT
                     TTPWIIEAIDNEIPFTAVCYSLNTFLALKEKPHCRAFLCGGEFHASNAIFKPIDFQQT
                     LNNFCPDIAFYSAAGVHVSKGATCFNLEELPVKHWAMSMAQKHVLVVDHSKFGKVRPA
                     RMGDLKRFDIVVSDCCPEDEYVKYAQTHRIKLMY"
     gene            complement(18694..19896)
                     /gene="dacC"
                     /locus_tag="ACHOSC_RS07385"
                     /old_locus_tag="pgaptmp_001477"
     CDS             complement(18694..19896)
                     /gene="dacC"
                     /locus_tag="ACHOSC_RS07385"
                     /old_locus_tag="pgaptmp_001477"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine-type D-Ala-D-Ala carboxypeptidase"
                     /protein_id="HGU4547451.1"
                     /translation="MTQYSSLLRGLAAGSAFLFLFAPTAFAAEQTVEAPSVDARAWIL
                     MDYASGKVLAEGNADEKLDPASLTKIMTSYVVGQALKADKIKLTDMVTVGKDAWATGN
                     PALRGSSVMFLKPGDQVSVADLNKGVIIQSGNDACIALADYVAGSQESFIGLMNGYAK
                     KLGLTNTTFQTVHGLDAPGQFSTARDMALLGKALIHDVPEEYAIHKEKEFTFNKIRQP
                     NRNRLLWSSNLNVDGMKTGTTAGAGYNLVASATQGDMRLISVVLGAKTDRIRFNESEK
                     LLTWGFRFFETVTPIKPDATFVTQRVWFGDKSEVNLGAGEAGSVTIPRGQLKNLKASY
                     TLTEPQLTAPLKKGQVVGTIDFQLNGKSIEQRPLIVMENVEEGGFFGRMWDFVMMKFH
                     QWFGSWFS"
     gene            20143..20769
                     /gene="gstB"
                     /locus_tag="ACHOSC_RS07390"
                     /old_locus_tag="pgaptmp_001478"
     CDS             20143..20769
                     /gene="gstB"
                     /locus_tag="ACHOSC_RS07390"
                     /old_locus_tag="pgaptmp_001478"
                     /EC_number="2.5.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308945.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase GstB"
                     /protein_id="HGU4547452.1"
                     /translation="MITLWGRNNSTNVKKVLLTLEELELPYEQILAGREFGINHDADF
                     LAMNPNGLVPLLRDDESDLILWESNAIVRYLAAQYGQKRLWIDSPARRAEAEKWMDWA
                     NQTLSNAHRGILMGLVRTPPEERDQAAIDASCKECDALFALLDAELAKVKWFSGDEFG
                     VGDIAIAPFIYNLFNVGLTWTPRPNLQRWYQQLTERPAVRKVVMIPVS"
     gene            complement(20766..21881)
                     /gene="yliI"
                     /locus_tag="ACHOSC_RS07395"
                     /old_locus_tag="pgaptmp_001479"
     CDS             complement(20766..21881)
                     /gene="yliI"
                     /locus_tag="ACHOSC_RS07395"
                     /old_locus_tag="pgaptmp_001479"
                     /EC_number="1.1.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldose sugar dehydrogenase YliI"
                     /protein_id="HGU4547453.1"
                     /translation="MHRQSFFLVPLICLSSALWAAPATVNVEVLQDKLDHPWALAFLP
                     DNHGMLITLRGGELRHWQAGKGLSAPLSGVPDVWAHGQGGLLDVVLAPDFAQSRRIWL
                     SYSEVGDDGKAGTAVGYGRLSDDLSKVTDFRTVFRQMPKLSTGNHFGGRLVFDGKGYL
                     FIALGENNQRPTAQDLDKLQGKLVRLTDQGEIPDDNPFIKESGVRAEIWSYGIRNPQG
                     MAMNPWSNALWLNEHGPRGGDEINIPQKGKNYGWPLATWGINYSGFKIPEAKGEIVAG
                     TEQPVFYWKDSPAVSGMAFYNSDKFPQWQQKLFIGALKDKDVIVMSVNGDKVTEDGRI
                     LTDRGQRIRDVRTGPDGYLYVLTDESSGELLKVSPRN"
     gene            complement(21992..22375)
                     /gene="bssR"
                     /locus_tag="ACHOSC_RS07400"
                     /old_locus_tag="pgaptmp_001480"
     CDS             complement(21992..22375)
                     /gene="bssR"
                     /locus_tag="ACHOSC_RS07400"
                     /old_locus_tag="pgaptmp_001480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biofilm formation regulator BssR"
                     /protein_id="HGU4547454.1"
                     /translation="MFVDRQRIDLLNRLIDARVDLAAYVQLRKAKGYMSVSESNHLRD
                     NFFKLNRELHDKSLRLNLHLDQEEWSALHHAEEALATAAVCLMSGHHDCPTVITVNAD
                     KLENCLMSLTLSIQSLQKHAMLEKA"
     gene            22588..23913
                     /gene="rimO"
                     /locus_tag="ACHOSC_RS07405"
                     /old_locus_tag="pgaptmp_001481"
     CDS             22588..23913
                     /gene="rimO"
                     /locus_tag="ACHOSC_RS07405"
                     /old_locus_tag="pgaptmp_001481"
                     /EC_number="2.8.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008172 - S-methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0016782 - transferase activity,
                     transferring sulphur-containing groups [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0018339 -
                     peptidyl-L-beta-methylthioaspartic acid biosynthetic
                     process from peptidyl-aspartic acid [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12 methylthiotransferase
                     RimO"
                     /protein_id="HGU4547455.1"
                     /translation="MSKVTPQPKIGFVSLGCPKNLVDSERILTELRTEGYDVVPSYDD
                     ADMVIVNTCGFIDSAVQESLEAIGEALNENGKVIVTGCLGAKEDQIREVHPKVLEITG
                     PHSYEQVLEHVHHYVPKPKHNPFLSLVPEQGVKLTPRHYAYLKISEGCNHRCTFCIIP
                     SMRGDLVSRPIGEVLSEAKRLVDAGVKEILVISQDTSAYGVDVKHRTGFHNGEPVKTS
                     MVSLCEQLSKLGIWTRLHYVYPYPHVDDVIPLMAEGKILPYLDIPLQHASPRILKLMK
                     RPGSVDRQLARIKQWREICPELTLRSTFIVGFPGETEEDFQMLLDFLKEARLDRVGCF
                     KYSPVEGADANALPDQVPEEVKEERWNRFMQLQQQISAERLQEKVGREILVIIDEVDE
                     EGAIGRSMADAPEIDGAVYLNGETNVKPGDILRVKVEHADEYDLWGSRV"
     gene            complement(23960..>25154)
                     /locus_tag="ACHOSC_RS07410"
                     /old_locus_tag="pgaptmp_001482"
     CDS             complement(23960..>25154)
                     /locus_tag="ACHOSC_RS07410"
                     /old_locus_tag="pgaptmp_001482"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009975 - cyclase activity [Evidence IEA];
                     GO_process: GO:0009966 - regulation of signal transduction
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HGU4547456.1"
                     /translation="AFAVANIAEYRRSVAIDLINTLNPTEEQLLVGLRTAYADSVSPS
                     YLYDVGPYLISSDECIQVKEFEKNYCADIMQVVKYRHVKNTGFISFDGKTFVYYLYPV
                     THNRSLIFLLGLERFSLLSKSLAMDSENLMFSLFKNGKPVTGDEYYAKNAIFTVSEAM
                     EHFAYLPTGLYVFAYKKDVYLRVCTLIIFFAALVAVISGTSCLYLVRRVINRGIVEKE
                     AIINNHFERVLDGGLFFSAADVKKLYSMYNSAFLDDLTKAMGRKSFDEDLKALPEKGG
                     YLCLFDVDKFKNINDTFGHLLGDEVLMKVVKILKSQIPVDKGKIYRFGGDEFAVIYTG
                     GTLEELLSILKEIVHFQVGSINLSTSIGVAHSNECTTVERLKMLADERLYKSKKNGRA
                     QISWQ"
BASE COUNT         6332 a         6544 c         6147 g         6131 t
ORIGIN      
        1 cttgatggca ttgttatccc atgccgcaga cacggccaaa aatcataaga ttgacagacg
       61 ggagttccat catgaaaaag ttagttcttg ccgctttact tgcttccttt actttcggtg
      121 cttctgccgc agagaaaatc aattttggcg tttcagccac ctatccaccc tttgaatcta
      181 taggtgctaa taatgagatg gtcggctttg atatcgatct ggcaaaagcc ttgtgcaaac
      241 aaatgcaggc agaatgtact tttactaatc acgcgttcga cagcctgatc ccgtcactga
      301 aattcagaaa atatgacgcc gtaatctccg gtatggatat cacgcccgag cgtagcaagc
      361 aggtatcgtt taccacgccc tactatgaaa actcagccgt cgtgattgcc aaaaaagata
      421 cctacaaaac gtttgccgat ctgaaaggca aacgtattgg gatggaaaac ggcaccacgc
      481 accagaaata tattcaggat cagcaccccg aagtgaaaac tgtctcctat gacagttatc
      541 agaatgcctt tatcgatctg aaaaatggtc gtattgatgg ggtatttggt gacacagcgg
      601 tggtaaacga atggctgaaa accaatccgc aactgggcgt tgctactgaa aaagtgaccg
      661 atccgcaata cttcggcacc ggtctgggca tcgctgtacg tccggataac aaagctctgc
      721 tggaaaaact gaataacgcg ctggcagcaa ttaaagctga cggtacatat cagaaaatca
      781 atgaccagtg gtttccgcag taattgagac tgatgacaaa agtaagattg cctgatgcgc
      841 tgcgcttatc cggcccacat caactctgca atgtattgat ttagcatgat tttgtaggcc
      901 ggataaggcg tacacgccgc atccggcatg aacaaagcgc acctgatcag caatctgaag
      961 ccgcacctgc ggctttttta ttgcttcacc agcagcgtca gcacttcata gtgcgcggtg
     1021 tgcgggaaca tatcaaaaag ctgtacccgt tcgatgcggt aacctggcag ttcgcggata
     1081 tctttcgcca tggtttgggc gttacagctg gagtagataa taaaacgcgg tgccattgtt
     1141 gagagataat cacatagcgg tttaccaatg ccgcggcgcg gcggattaac cagcaccagc
     1201 tccggcacct ccccctgagc ggtggcaaac tgagtggagt ccagcgcctg aaattgcaaa
     1261 cgcgttaagc ccagttcagc ggctgactgc tttgcacagg caatggcctc tgatgcaatt
     1321 tcgatcccgg ttaactgcat gtcaggcgtc gcgcagtgta aaccaaagcc acccacaccg
     1381 cagaacagat cccacatatg tttaactggc agctgccgta cccagtcgcg cgcggtagcg
     1441 tacagctggc tggcgaccgc cggattagtc tggaagaaac tttgcggacg gatccacagc
     1501 ggtacgtcat taaaacgctc tgccagcgcc tgttgttcag taaggtagat ctccgtctcc
     1561 ccttccataa tcgccatatg taccggctga atattgacgg taataacttt cagttgcggt
     1621 agttgttcct gtaaccacgg cagcgcctta cgcagttgtg ccagtttggt ttcagaacgc
     1681 agtacaaagc gcagcatcat gccgccatcg ctctggcttt cagtcagcag aatgtatttc
     1741 agttcgccac gcttacgcgc cacgttgtag ggtgtcagcc ccgcacgggc gataaacggt
     1801 tttagcgccg caaaaacggg cgcaaatgag gcaggataaa gcgggcagtc acaaaggtct
     1861 tctggtgtgc cgtcgcgatg cagcataccg agcagtggtt tttcaacgct accactcacc
     1921 accattttgg ctttattacg aaacccttgt tccgggcctg acaccggcgc gcaccattcc
     1981 tcaaccggaa aatcggcgag cagattttta agatcggcgg ttttagcgga gagttgctct
     2041 ggaatcggct gcgttatcca ctgacaggaa cgacagcgac ccgcgtcgta aagtgcgcac
     2101 tgcatacatt gaccttcaca tcatcagggg cgacgattat acacattatt gcaactggaa
     2161 gaaccgccga ctggtcgagg gaacgaacag cagcatcagg atgagcatat ccggcagctt
     2221 ctgcagcatc aggctatgga agatttcacg ttttgattca ccgggaatgc tgaacagctc
     2281 cggataaccg taccccagcg aagccgccca caagtaactt gcggcggtga tttgtgtcag
     2341 cagatatagc cagcgcgccc agcgacgccc tttcaccagt gaaaacgcac accagatctc
     2401 aatgaaaacc agcaccagac tgcttaaaaa gaccagcgtt aaactccagg tttgtacgct
     2461 gcgatgaatg aactcgccaa taccgcgcac gcccagcgta ttgaaaatca tcagcacgtc
     2521 gaggccacgg atcataataa tggcgagcgc cgccacctgc accagcgcag gcacattcag
     2581 gcgagcatga gatgaagatg ttttcttaaa aaatcccaac gtttcgtctt ccatgaaaac
     2641 aatgccgcgg catgcgcggc attatgtagc caggttggca aattttagtg tcttcagcca
     2701 tgtcttgcac gctggatatc gcgtatccgc tgtttttctg cgcgagccat cagataccag
     2761 gcgataaatc cgacaattcc gaccgcaccg agaattaacg ttgcaagggc gttgatttcc
     2821 ggattcaccc ccatccgcac gctggaaaag accagcatcg gtaacgtggt ggctcccggc
     2881 ccggaaacaa agctggcgat caccagatca tcaagcgaca aagtaaaagc cagtaaccag
     2941 ccagaaatga tcgcgggcat gatcatcggt agcgtaatga cgaaaaacac tttcagcggc
     3001 gtcgcaccga gatccatcgc tgcttcttct atcgagcgat ccagttcccg caatcttgaa
     3061 gaaataacga ccgccacata agccgtacaa aaagtgacat gcgccagcca gatggtgagc
     3121 ataccgcggt ccgcaggcca gccaatggca tgagcaagcg cgacgaataa taacaacagc
     3181 gacaagcccg tgatgacatc tggcatcacc agcggcgcgg tgatcataaa ggcaaagcca
     3241 tttgatccgc gaaacctgcc aaagcgcacc agcaccaccg ccgcaatagt cccgaggatc
     3301 gccgccgccg ttgccgcaca ggcagcaatt gtcaggctta aaccaaccgc actcatcatc
     3361 gcgtcatcgc gcaataactc accataccag cgcgttgacc agccggccca caccgtcacc
     3421 agcttcgagc tgttaaacga atagataacc agcatcagca ttggcgcgta gagaaaggta
     3481 aagcccagca gcaaaatcac aatccgccag ggcgaacgaa ctaccggtaa attattcatc
     3541 cgtgttctcc cacgcttttt tgctggtgtt tgtggaacca cattatcggc acaattagca
     3601 gcagcaacat gatgatcgct accgccgagg ccaccggcca gtcgcggtta ttgaaaaact
     3661 cttgccatag cacgcgcccg atcatgatgc tgtccgggcc accgagcagt tccgggatca
     3721 caaactcgcc cacagccggg ataaacacca gcatcgatcc ggcaataatc ccacctttag
     3781 tcagcggcac gatcacagta aagaacgttt tcagcggtcg tgcaccgaga tccagcgctg
     3841 cttccaccag cgaataatca atacgaatca acgcggtata aatcggcagt accataaacg
     3901 gcacgtaggc gtaaacaatg ccaatataaa ctgccagatt ggtatgaaga atggtcagcg
     3961 gttgatcgat aacccccagc cacagcagaa aattattcag cacaccgttg ttttttaata
     4021 ttcccatcca ggcataaacg cggatcagaa acgaggtcca cgacggcagg atcaccagta
     4081 gtaataaaat attacgggtc gaaggcttac tgtgcgccac cgcccacgcc agcggatagc
     4141 cgatcagtaa acagcaaaat gtcgaaatcg cagccacctg tagcgactgg agataagcat
     4201 cgaaatagag cggatcgtcg gtcagttgca gaaaattacc gagattaaga gtgatggaca
     4261 gttgcccgtc agcccactcc atcagttcgg tataaggtgg aatagcgcgc gccatctccg
     4321 ccaggcttat tttaaagaca atcagaaatg gcagcagaaa cagcaagatc aaccagatat
     4381 atggcaacgc aatgaccagt ttgcgcccat gcttcatttg cagctgcgac aaccacagct
     4441 taaatccgcc cggcggtttc gactgggcag caggttcaag tgtattcatc tttgctcctt
     4501 aaaccgtcag caccacacag ctgtccactt cccagcacaa acgcacttcg tcgccccagg
     4561 tcggtaaccc tttacgatga cgatgggcgt tttgtagctg ggcgctaatc atctgcccac
     4621 ttttcagacg aacgtgatac accgaaagat cgccgagata ggcaatgtgt atcacctccc
     4681 ccaccgcgaa gttacaacca ttggcgggcg gctcttcgca aagcatgatt ttttccgggc
     4741 gcagcgctac gtgtaccggc acgttatcga ccactgaagc atccgcatcg actttcagtg
     4801 gatgcaccag ccccggcgaa tcaagcacca ggccatcttc ctgacgctct ttgagtacac
     4861 cttcaaagac attaaccgaa ccgataaatt cagcgctgta gcgggtagtt ggatgctcgt
     4921 agatctcttc cggctcgcca atctggacaa atttcccgcg attcataatg gcgatacgcc
     4981 ccgccatggt catcgcctct tcctgatcgt gggtgaccat cacacaagtc acaccgacgc
     5041 gctccagaat atccaccact tcaagctgca tccggtcacg cagcttttta tccagcgcgc
     5101 ccatcggctc atcgagcagt aatagtttcg ggcgtttggc aaggcttcgg gccagggcca
     5161 cacgctgtcg ctgaccaccg gaaagctgat gcggtttgcg tttggcgaac tcctgcatgt
     5221 gcaccagccc aagcatctcg ttgacccggg tggcaatttc cgctttcggt agtttgtcct
     5281 gtttcaggcc aaaggcgatg ttctgctcca cagtcatatg gggaaacagc gcgtaagact
     5341 gaaacatcat attgatgggg cgcaggtaag gcggcacctg tgacaaatcg acgccatcaa
     5401 gcattatctg tccggcagaa ggttgttcga aacctgccag catacgcagc agcgtggact
     5461 tgccacagcc ggatgcgccc agcagcgcga agatttcacc tttgtagatg gtcaggctga
     5521 catcatccac cgcatgttga ccatcgtagg atttggtgag gttgcggatt tctaatagcg
     5581 gcgtcagcgc cttacgggtt ttcgcctgcg ggcgagggat agcgtcattc acggctgctc
     5641 tccggcattg aatgtgtgca agcgcaccat gatggtgcga attgccggcg ggtgtggtgc
     5701 gcccgcccgg catctaaggc tgcggattat tttccgctct tcactttggt ccacgcgcgg
     5761 gtgcgcacac ggtcgatttt cgggtcctgc actttcagag tgaacagctt cgcacgaaca
     5821 tccgcaggcg gataaatacc cgggttctca cggacttccg cactcaccag cggcgtggct
     5881 gctttattgg cgttggcata gaacacattg tcggaaatat gcgctactac atccgggcgc
     5941 agcaggtaat tcaggaactg ataggcttcg tctttgtttt tggcatccgc aggcatggcg
     6001 aatacatcaa agaacgccat cgccccttct tttggaatcg agaacgagac attcactcca
     6061 ttcttcgctt ccttcgcgcg gtttgacgcc tgccagacat cacctgccca gccgatagcg
     6121 acgcaaatat cgccgtttgc caggtcgtta atgtattgag atgaatggaa ataacgaatg
     6181 ttcgggcgca gctttaacag cagatcggtt gccggtccgg tgtaatcatc cgctttagtg
     6241 ctgttgggat ctttgccgag ataattcaac acggtagcaa aaacttcttc tggcgcatcc
     6301 aggaaagaga caccgcagct tttcagtttt tccagatttt caggtttgag gatcaagtcc
     6361 cagctatcga cgggcgcgtt ttcgcccagc acagctttaa ctttatcaac gttatagcca
     6421 atcccggtcg tcgcccacat atagggcata gcaaatttat tgtcgggatc gtgtttggcg
     6481 accagcttca gcagctccgg gtcgagattc ttccactccg gcaatttgct tttgtccagc
     6541 ggctggaaaa ctcccgcagt caactggcgt tcaagaaagc tggcagatgg aaccaccaag
     6601 tcaaagccgg tgctacccgc cattaatttg ccttccagta cttcgttaga gtcgaaaaca
     6661 tcgtagacga ctttaatacc ggtttctttt tcaaaattgg ccaccgtgtc cggggcgata
     6721 taatcagacc agttataaac gtggagtgtt ttttgttcag ccgcgagcgt gccgacagag
     6781 acggccatca gagcacccgc agccagaccc gaaagccatt ttttatttaa ggcggtcata
     6841 tctctttcct tctgaaagtt cgttaacaaa cgaacaaaag ttgtcacact aaaagatatg
     6901 caataaacgt gcatattttt tatcggcggg gtgtaagaaa agagaactct ctcccggcca
     6961 gcactgctcg ctatataaaa gcatcgcaag aataacttta gccagggttt gagactatag
     7021 ctcggattaa aaaacggtat ttaacaaatt tttatgcgta tttgctctat gtgataaagc
     7081 caaatcgaca caagagaagt gaaataatct ttaagaaaag gttaaaaaca gcagataatt
     7141 ttgcagcgcg cagccactgc accagcgact gcgtgctcga aaggagttat cagtggagga
     7201 aatagttttc ggtaacgttg ttttgccctt catcgtcagt cggtaacagc agttgatggg
     7261 cattagcttc aagaatgacc atcgaaatct gctcttcgct ctgacgcacg aaccatgcaa
     7321 actgctcata ggttacgccc tgcataacgg ataaagactg gcaaaccacc agctttggca
     7381 gattatcatc ctgcatatca agaaatgctt tcacggtcag cgaactggca ttgatggcag
     7441 ataaatccgc cgccagcggc aatacggctg aaggtcggac ttccgccatg gcagaaaaca
     7501 ggatcgtgtt atcgatcaga tcgattttgg catcaaagac accgtcgaaa ttctgcatat
     7561 ggggcagatg cagagcctga cagttatcac attcaaaaaa actcatcccc aggtcatcga
     7621 gccattgacg cagcgtatcc agaccaggaa cgaccagcga tgtcataatt ttgtacaacc
     7681 tctttcgata aaaagaccgg cacagcttac gcaaaaagcg caggcaaaac catgatcagt
     7741 aatgtgattg cgactaacca cccgttttca ggcaatattc tgtcgtagcg tggcgttcga
     7801 tccagcggat cattttaccc gcgatgtcga taccggtggt tttttctatt ccttccagcc
     7861 ccggcgacgc attcacctcc atcaccaacg gcccgcgatt agcacgcaga atatccacac
     7921 cagcaacgtc cagcgccatc gttcgcgcgg cttttatcgc gatttcacgc tcctgtggtg
     7981 tgatactggc gacgcttgcc gcgccgccac gatgcaaatt ggaacgaaaa tcgccctctt
     8041 tcgcccgccg ttcaatcgca gcgaccactt catcgccaac aaccagacag cggatatcgc
     8101 acccttgcgc ctctttgata tattcctgca ccagaatatg cgcgttcaga ccgcggaaag
     8161 cgtcaatcac gctttccgcc gcctgacgcg tctccgccag cacgacgcca attccctgcg
     8221 tgccttcaac caacttgacc accagcggcg caccaccgac catgtcgatt aaatcgctgg
     8281 tatcatccgg cgaatgcgca atgcccgtga caggcaggtc gatgccctga cgcgccagca
     8341 gttgcatgga acgcaatttg tcacgcgccc gggcaatggc gaccgactca ttgagcggat
     8401 agctccccag catctcgaac tggcgcagtg ccgccgtccc ataaaaggta atggcggtgc
     8461 caatacgcgg gatcactgcg tcaaaatggg gtaacttgcg gcctttgtag tgaatagaag
     8521 acgccgcagg atttatgttc atgtagcaag aaagcggatc aagaatttca accaggtgac
     8581 cgcgctgtat agcggcttca cgcagccgct tacacgaata gagcgttcca tcccgggaca
     8641 atatggcaat tttcaccctg cacctctctg tcagacctgg tcaaaagccc gcgtatcata
     8701 catcgacgtg gcggttttag cgcgtcgccc aaccctgttt gtgcaaataa tccagaataa
     8761 atgggcggct ttctttaatg attgttcggc ggatatgatc gctccaggta tcccggcgat
     8821 tattgctgcc acgggtgagg taatattccg ccagttgctc gtcatactgc gccagtgcgc
     8881 ctttatccag cggttgatag ctgttttcat gcaccaaaat ggaggccggt aaacgcggct
     8941 taagatccgg attatccgca ggccagccaa ggcacagccc aaacagcggc agaacatgct
     9001 gcggtaattt aagcagtttc gtcaccgctt caatattatt gcgcaggccg ccgatatata
     9061 ccccgcccaa tcccagcgat tccgctgcga ttaatgcatt ctgcgccatc attgccgtat
     9121 caacgacacc gagcaacagt tgttccgcca ggccgagctg agcatccgga cagatctgta
     9181 aatggcggtt aaagtcggca cagaacaccc agaactccgc cgcttgcgct acgtgttttt
     9241 gcccgccggt cagcgtcacc agttcttcac gtaacgcttt gtcggtaatg cgaataatgc
     9301 tactgcactg caaaaaactg gaactggacg tcgcacgggc gctgttaata atcgcctcac
     9361 gctgcgcttc ggaaatgggt tcatcagtga aatggcgaat ggagcgatgg ccacaaataa
     9421 gttcaatggt tggcgtcatt atctttttct ctttctgaac gtgaatattg cggtggacgg
     9481 ttcatcagct gtggggcaag acgttttgcc acctgaagaa taaccaccac cgcagcggga
     9541 agcatgagca aaacaccgag aaaaatcatc agaatctgca cttctggccg agaaaatggc
     9601 tcaggcagcg acagggagtc gcttaccgac agcagcgcca ccgccagtag catcattccg
     9661 ataaattcca gtatcaacac gcctttaggc aatttaccga tcgcgcgcat acgcttccct
     9721 ctgcaaagtg agccttcagt ctaaaacttt tcactgtatt gtgtttaaca gttatagctt
     9781 ttagcaatta atgcaacagg ttaaacctac tttcagcgaa tacattttag cgtgatcatt
     9841 acaggcataa atctatgagg agagaaataa tgcaaaccgt tatttttggt cgttcgggtt
     9901 gcccttactg tgtgcgtgca aaagatctgg ctgagaaatt gagcaatgaa cgcgatgatt
     9961 ttcagtatca gtatgtagat attcgtgcgg aagggatcac taaagaagat ctacaacaaa
    10021 aggcaggtaa acccgtagaa accgtgccgc agatttttgt cgatcagcaa catatcggcg
    10081 gctataccga ttttgctgca tgggtgaaag aaaatctgga cgcctgatcg tctgacaagc
    10141 cctcgcgttg agggctttac tgattttttc tgtgctgtgg tttaaacaaa ctactgataa
    10201 ataagaaaca cagtgccccc agcgcacacc agaacaccgc gcttagtaac catgccagct
    10261 cttgccagaa tgagcgcgtc ggtgaaaaaa acagccgcat aatgagcatt gaacagggtg
    10321 ccgccagcat tgcgccaaat agaggtttca ggacttctct acgctgtgaa aagaagctgg
    10381 cgactgctcc aggaagaatg aaaaatagca agccgatttc aggatgcccg gcagcccgaa
    10441 aagcgccttt catgtgcgtc gccagaaaaa ggcacaccac aatgaagagg acaaaacagc
    10501 agattgcccc cgcccaacgt tgtttatgtt tcactcgttc ctcctgacac tgcgtctatc
    10561 gaacacattt ttcgccagtg tggcgttcag taagataaag ccgcttcgca ttccatgcta
    10621 atataggcca acgcaattca tatagccgtt gatacctaat gtgattacac tagtaaaata
    10681 tattgttact ttactatcgt ttaggtgcgc tgaatgaatc tgcgccctga attctggtaa
    10741 aaaacattat cgtaaattac catttctttc aacagcttac tagtaaacaa gaagttagcc
    10801 tccgtgaata taaacgtcgc cgaattgtta aatgggaatt acattctgtt attatttgtg
    10861 gtcctcgcgc ttgggctatg tctcggaaag ttacgacttg gttcgatcca actgggtaat
    10921 tccattggcg ttttagtcgt atcgctgtta ttaggccaac aacatttcag cattaacacc
    10981 gatgcgctta atcttggctt tatgctgttt attttctgcg tcggggtcga agccggaccg
    11041 aacttttttt ccattttttt tcgcgatggg aaaaattacc taatgttagc actggtgatg
    11101 gttggcagtg cgctggtgat cgccttaggg ttaggtaagc tgtttggctg ggatattggc
    11161 ctgacggccg gtatgttagc aggctctatg acgtcgacac cggttctggt cggtgctggc
    11221 gatacactgc gtcattccgg catggaaagc aggcagctct cactggcact ggataatctg
    11281 agcctcgggt atgccttaac ctatttaatc ggtctggtga gtttgattgt tggtgcgcgt
    11341 tacttgccga aattgcagca tcaggactta cagaccagcg cccagcaaat cgcccgcgaa
    11401 cgtggcctgg acactgatgc caaccgtaag gtttatttac cggtgatccg cgcctatcgc
    11461 gtcggcccgg agctggtggc ctggaccgac ggcaaaaatc tgcgtgaact gggtatttat
    11521 cgacaaaccg gctgctacat tgaacgtatt cgacgtaacg ggattctggc aaatccagac
    11581 ggtgatgccg tgctacaaat gggcgatgaa atagcgttgg taggctatcc cgacgcccat
    11641 gcccgactcg atcccagctt ccgtaacggt aaagaagttt tcgatcgtga ccttctcgac
    11701 atgcgtatcg tcactgaaga agtggtcgtt aaaaaccata acgctgtagg taaacgtctc
    11761 gcacaactga agttgaccga tcacggttgc ttccttaacc gcgtcattcg tagccagatt
    11821 gagatgccga tagatgacaa cgtcgtgctt aacaaaggtg acgttttaca agtcagcggt
    11881 gatgcccgtc gcgtaaaaac catcgccgat cgcatcggct ttatctcgat tcacagccag
    11941 gtcactgacc tgctggcatt ttgcgccttc tttgttattg ggctgatgat cgggatgatc
    12001 accttccagt tcagcacatt cagtttcggc atggggaacg ctgccgggtt gttattcgcc
    12061 ggaattatgc tgggctttat gcgtgctaac cacccgacct tcggttacat tccgcaaggt
    12121 gcattaagca tggtgaaaga gttcggcttg atggtgttta tggcaggcgt tggtctgagc
    12181 gccggtagcg gtattaataa cggcctgggc gcgattggcg gtcagatgtt gattgccgga
    12241 ttaattgtca gtcttgtgcc cgtggttatc tgtttcttgt tcggtgctta tgtattgcga
    12301 atgaaccgcg cactgttgtt cggcgcaatg atgggcgcac gcacctgcgc gccggcaatg
    12361 gagatcatca gtgatacagc tcgcagtaac atccctgcgc tgggctatgc gggcacctac
    12421 gcaatcgcca acgtcctgct gacgctggca gggacaatca tcgtcatggt atggccagga
    12481 ttaggataaa actgaagttg ccctgaaaat gaaatttttt tgcacaaccg cagaactttt
    12541 ccgcagggca tcagtcttaa ttagtgccac tgcttttctt tgatgtcccc attttgtgga
    12601 gcccatcaac cccgccattt cggttcaagg ttgatgggtt ttttgttatc taaaatttat
    12661 ctactaccct gcaaccctct caaccatcct caaaatctcc tcgcgcgata gcggcttacg
    12721 gtcggtgaca aaatgcagcg tcatcccctc aataaacgca tcaagcgcgc gggcggttcc
    12781 gggttcaaac cattgttcga gcgtttgctg actgcgctgc atccagtttt gcattaccgt
    12841 ttttaatagc ggttttcggc tagccagcgc gtagagctgg tacatcagct ccatgttatc
    12901 cggcgttgca acctgtgagc tgtagatcat atcggtgata gcctggcatg cgccttgagc
    12961 atcactaaca tcgctaaaaa atgcctgata ttgccgggac ataatctcag taaaactgct
    13021 gaacgcctcc agcaacaact catcaattcc tgaaaaatag taggtcatcg atcccaacgg
    13081 taccccggca agggtagcaa ttttgcggtg cgtaacagca tgtattccgt aaagtttcac
    13141 cgcctccagc gtggcctgga taattttttc tcgccgttgc ggatcgttag cgcgacgcat
    13201 gtcatttcct ctgtctgcaa ttgtgtacaa atgtacacaa ccttgctaat gttgtcttcc
    13261 ctcttcttat tttgacgtga tctatgaccg taaattcttc acgtaatgca ttgaaacgcc
    13321 gaacctgggc gctgtttatg ttcttctttt tgccgggcct gttaatggcg tcctgggcaa
    13381 cccgtacgcc tgctatccgc gatattctct ctgtctcgat cgctgaaatg ggcggtgttc
    13441 tctttggtct gtcgatcggt tcgatgagcg gtattctctg ctcggcgtgg ttagtgaaac
    13501 gctttgggac acgtaatgtc atcctggtca cgatgtcctg cgcattgatc gggatgatga
    13561 tattaagtct ggcactctgg ctgacatcac ccctgctctt cgcggttggc ctcggcgtct
    13621 ttggggcaag ttttggttct gcggaagtgg cgataaacgt cgaaggtgcc gccgttgagc
    13681 gagaaatgaa taaaacggtt ttgccgatga tgcacggttt ttatagcctg ggcacgctgg
    13741 caggcgctgg tgtcgggatg gcactgacgg cctttggcgt tccggcaacg gtgcacattt
    13801 tattggcggc gctggtagga atcgcgccta tttatatcgc cattcaggca atccctgacg
    13861 gtacgggcaa aaatgctgcc gatggcaccc agcatggtga aaaaggcgta cctttttatc
    13921 gcgatatcca gttgctgctg attggtgttg tggtgctggc gatggccttt gccgaaggtt
    13981 ctgccaacga ctggttaccc ttattaatgg ttgacggtca cggttttagc cctacttccg
    14041 gctcgctgat ttatgccggt tttaccctgg ggatgactgt tggacgcttt accggcggtt
    14101 ggttcatcga ccgttacagt cgcgttgccg tggttcgggc cagtgcacta atgggagcgt
    14161 tgggtattgg gctgattatt tttgtcgata gcgcctgggt cgctggggtg tctgttgtac
    14221 tctggggact gggtgcctcg ctgggcttcc cgctgaccat ttctgccgcc agcgataccg
    14281 gccccgatgc accaacccgc gtcagtgtgg tagcaacgac cggttatctg gctttcctcg
    14341 tcgggccgcc gctgctgggc tatctcggcg aacattatgg attacgtagt gcaatgctgg
    14401 ttgtactggc gctggttatt ctcgcggcta ttgtcgcgaa agccgtcgcc aaacccgata
    14461 ccaaaacgca gacggcgatg gagaatagtt gatgagcatt aaattaattg cggtagacat
    14521 ggatggtact ttcttaagcg atcaaaaaac ctataaccgt gagcggttta tggctcagta
    14581 tcagcaaatg aaagcacaag gcattcgctt tgtggtcgcc agcgggaatc aatattatca
    14641 gttgatctct ttcttccctg aaattgcgaa tgaaattgcc tttgtggctg aaaacggcgg
    14701 ctgggtagtg agtgaaggca aagatgtttt taatggcgag ctgtcgaagg atgcgtttgc
    14761 tactgtcgtg gaacatttgc tgacgcgccc ggaagtggaa attattgcct gcggaaaaaa
    14821 tagcgcctat acactcaaaa aatatgacga tgccatgaaa acggtggcgg aaatgtatta
    14881 tcaccgtctg gaatacgtcg ataactttga caacttagaa gatattttct ttaagtttgg
    14941 cctgaatctt tccgatgaac tgattccaca agtacaaaaa gcattacatg aggccatcgg
    15001 cgatattatg gtgccagtcc acaccggcaa cggcagcatc gatctgatta tccccggcgt
    15061 acataaagcc aatggccttc gccaactgca gaaattatgg ggaatagacg acagcgaagt
    15121 ggtggtcttt ggcgatggcg gtaacgatat tgagatgctg cgtcaggcag gctttagttt
    15181 tgcaatggaa aatgccggca gcgcggtcgt cgcagcggca aaataccgcg caggctccaa
    15241 taaccgtgaa ggcgtactgg atgtgatcga taaagttctt aaacacgaag cgccatttga
    15301 ccaataaaaa cggcagcacg cccggcaaca caatgcctga atgaaggctg cataattcgc
    15361 ggaaattaac ccgctgcatt atttcaggcg aaatgattat gaaaaattgt ctactgttgg
    15421 gcgcgctttt aatgggcttt actggtgtgg cgatggcgca aagtgtcacc gtcgatgtgc
    15481 cgagtggata caaagtggtt gtggttcctg attcagtcag cgttccacaa gcggtcagcg
    15541 tcgcgactgt gccgcaaacc gtctatgtgg ctcctgcgcc agctccggct taccgcccgc
    15601 atccttatgt tcgccatctt gccagcgtag gtgaagggat ggttatcgaa caccagattg
    15661 acgaccatca ccattaattc gagaatgcct gatcgcacaa agcaatcagg cattttttta
    15721 cccttcgtga gaatttccca tctgtttatc ttttaaaaag ataaccatca gcgacagcca
    15781 caaaattccg ttgacaaggt tgaagagatt aaacagtccg ttgcccccgt tcagccaggc
    15841 atgtttgctg atttcaatac cgacggtaaa gatcagcatt tgcagcattc ccatcgccgc
    15901 agaaaccgta cctttactca tatcgctggc aaacagggtt aatcgcacca gtcccgcatt
    15961 cgccagacca atgccgaaag cataaatact taacccggcg gtcatccata aatacgcgtg
    16021 cgatgagata accgttgccg cagcagcgac caacagacca atcataatcg gccagccgcc
    16081 cataattatc agcgaacgta cggtgcggcg cgaggtcaaa cgcgctaaca acaagttacc
    16141 cgcaattaac gccccgaaaa taggcacttg cagcaagcca tattcatagc tgctcaactg
    16201 ctcgccggta atgatgataa tcggcgactg ggcgatccac gccagcaatg gcaggctaac
    16261 gaatcccagc gccagcgccc ccgccacaaa gcggccgttc ttcagcacca gcttatagtc
    16321 acgaccgagt tctttcagtg acagtttctc gcctatacgc gtggcggttt caggcatggc
    16381 ttgttgcaga ccgaaaaagg agatcgctgc caatgcggca aacaagacaa acatcccttc
    16441 ccagggcagc acatggatcc acgccgcgcc caccagcgga ccaagtagcg gagcaatcag
    16501 cgccacgttc gccatcagcg cggtgatctt gatacaaacc gcctcttcga aggattcctg
    16561 aattgcggcg tatcccacag cgccaatgaa acagaggctt atgccctgca agaagcgcaa
    16621 cagagtgaat tgttcaatgt tttgcgccag taatattgcc agacaggtga tgataaacca
    16681 caccaccccc gccagcatca ccggacggcg accaatacga tccgacagcg gccccaggag
    16741 ccattgtaaa aacatcccgc ccgccagata tgcggtcatt gaagtaggaa cccaatcaat
    16801 gcccgcctga tattgttcca ccacggccaa cataccgggt tgaatcatat cgttgccgat
    16861 ataggttgaa aattcgtaaa gcaccagaca gagagggaaa agtaacgcct gacgtccaag
    16921 cctggcaccg gaagctaatt tattttgcat gcaatttctt cgccaataat aatcgcgcag
    16981 agtttaataa aagcacagct aacgagaaag cgaattttgt agctgaaacc acggttaagc
    17041 acattcttac attattgcga gtatagctac gctttcttta agttttattt aacctatgcc
    17101 cgttacaatt acccaccgta aacaggccgc ttgagggaaa taagacgatg ccgctttacc
    17161 cagtttaacc tgcactttat tctcaacgac ttgcctgtat tggctccctt ttaatcactt
    17221 tgcgtcggga agttatgctg gaaaatttga atctctctct attctctctt attaacgcga
    17281 cgccagactc ggctccgtgg atgatctcgt tggcgatttt tattgctaaa gatttgatta
    17341 ccgtggtacc gctgctggcc gcggtgcttt ggttgtgggg gcttacagca caacggcaac
    17401 tggtgataaa aatcgctatc gcgctggcgg tcagcctgtt tgtgtcctgg acgatgggac
    17461 atctttttcc gcacgaccga ccctttgtcg aaaatatcgg ctataacttc ctgcatcatg
    17521 cggcggatga ctcattccca agcgaccacg gtacggtgat tttcaccttt gcactggcat
    17581 ttttatgctg gcatcgcctg tggtccggct cacttttaat ggtgctggcc gtcgtcattg
    17641 cctggtcgcg cgtttatctt ggcgtgcact ggccgctgga tatgctcggt ggattgctgg
    17701 ctggtatgat tggctgcctt agcgctcaga ttatctggca agcgatgggg cataaactct
    17761 atcaacgtct gcaatcgtgg tatcgcttct gttttgcatt accgatccgc aaaggctggg
    17821 tgcgtgactg atctctgcta aaaagtgtag tattgagcgg ctcgcttcaa taactattca
    17881 gagggattat ggaaacacgt cgcgaagagc gcatcgggca gctgctgcaa gaattaaaac
    17941 gcagcgataa gttacatctt aaagacgccg ccgccctgct tggggtttcg gagatgacga
    18001 ttcgtcgcga tctgaacaac cacagtgcgc ccgtcgtttt gctcggcggc tatattgttc
    18061 tggaaccgcg cagtgccagc cattacctgt taagcgatca aaaatcccgc ctggtggaag
    18121 aaaaacgccg ggcggcaaaa atggctgcga cactggtaga acccgatcag actctctttt
    18181 ttgactgtgg caccaccacg ccgtggatta ttgaagcgat tgataatgaa atccctttta
    18241 ccgccgtttg ttattcgcta aatacctttc tggcgctgaa agagaaaccc cattgccgcg
    18301 catttctgtg cggtggtgaa tttcacgcca gcaacgccat tttcaaaccc atcgattttc
    18361 agcaaacgtt gaataatttt tgcccggata tcgcttttta ttccgcggcg ggcgtgcatg
    18421 tcagtaaagg cgctacctgt tttaatcttg aagagttgcc ggtaaaacac tgggccatgt
    18481 cgatggcgca aaagcatgtg ctggttgtcg accacagtaa atttggcaag gtgcgtccgg
    18541 cgcgcatggg tgacctgaaa cgctttgata ttgtggtgag tgattgttgc ccggaagatg
    18601 agtatgtgaa gtacgcgcag acgcaccgta ttaagttgat gtattaatga cgtataaccg
    18661 gatgacgttt cgcgccatcc ggttatcaga agattaagag aaccagctgc cgaaccactg
    18721 atggaatttc atcatcacga aatcccacat ccgaccaaag aatccgccct cttccacatt
    18781 ttccatcacg atcagcggac gctgctcaat ggatttaccg ttaagctgga aatcaatggt
    18841 cccgacaacc tgaccttttt tcagcggtgc ggtaagctgc ggttccgtta acgtgtagct
    18901 cgccttcagg tttttcagct gcccacgcgg tatggtcact gagcccgctt cgcctgcccc
    18961 aagattcact tcgctcttat caccaaacca gacgcgctga gtcacaaagg tggcatcagg
    19021 tttaattggc gtcacggttt caaagaagcg gaaaccccag gtcaataatt tctcagactc
    19081 attaaaacgg atacggtcag ttttcgcccc cagcactacg gagattaaac gcatatcgcc
    19141 ctgggtagcc gaagcaacca gattatatcc cgcgcctgcc gtggttcctg tcttcatgcc
    19201 atcaacattc agattgctgc tccacagcag acggttacgg ttaggctgac gaattttgtt
    19261 gaaggtgaac tctttctctt tatgaatggc gtactcttcc ggcacatcgt ggatcaatgc
    19321 tttacccagc aatgccatat cgcgcgcggt actgaactgc cccggcgcat ccagaccgtg
    19381 caccgtctgg aaggtagtgt tggtcagacc cagttttttg gcataaccat tcatcaaacc
    19441 aatgaatgac tcctggctcc cggcaacgta atcggccagc gcaatacagg cgtcattacc
    19501 ggactggata atcacacctt tgttcaagtc tgccaccgaa acctgatcgc ccggtttgag
    19561 gaacattacc gatgaaccac gcagtgccgg atttcccgtc gcccaggcat ctttaccgac
    19621 cgtcaccata tcggtgagtt taatcttatc ggccttaagc gcctgcccaa ccacatagct
    19681 ggtcatgatt ttagtcaggc tcgcgggatc cagtttctca tccgcgttgc cttctgccag
    19741 cactttaccg ctggcgtaat ccattaaaat ccatgcacgc gcatccacgc tcggcgcttc
    19801 aacggtttgt tccgccgcga atgccgttgg ggcaaaaagg aataaaaatg cagaacccgc
    19861 tgcaagacca cgaaggagag aggagtattg cgtcataaat gccacccgag tatccattca
    19921 aaaaaaatac gttatatcgc cgtatgataa gttcggtgat taataacgca cacacaaact
    19981 taaagaaacc gtaagttggt aaagttttta aagtttatgc aatatcctgc cgcttcgctg
    20041 cttctggtgt catttttttg ccagctgttg cttaaaagtt aactttactt caccatcgga
    20101 gtcctgttca gcctgtggct gcctgtcgga aaaggggtga gtatgattac gctgtggggt
    20161 cggaataatt caaccaacgt aaaaaaagta ttgctgacgc tcgaagaact ggaactacct
    20221 tatgagcaaa ttctcgcggg ccgtgagttt gggataaatc acgatgctga ttttctggcg
    20281 atgaacccta acgggctggt gccgttgttg cgtgacgacg aaagtgatct cattctttgg
    20341 gaatcaaacg ccattgtccg ctatctggcg gcacagtacg ggcaaaaacg tctgtggatc
    20401 gactcaccgg cacgtcgtgc ggaagcggaa aaatggatgg actgggcaaa ccagacgctc
    20461 agtaatgctc atcgcgggat cctgatggga ttagtcagaa caccaccgga agagcgcgat
    20521 caggccgcca ttgatgccag ttgcaaagag tgcgacgccc tgtttgccct gctcgatgcg
    20581 gaactggcaa aagtaaaatg gttctctggc gacgagtttg gtgtgggcga tatcgctatc
    20641 gcaccgttca tctacaattt gtttaacgtt ggcctgacct ggacaccgcg tccaaatctg
    20701 caacgctggt atcagcaact cactgaacgc cccgcagtgc gcaaagtggt gatgattccc
    20761 gttagctaat tgcgtgggct aactttaagt aattccccac tggactcgtc ggtgagaacg
    20821 tataaataac cgtcgggtcc agtgcgaaca tcacgaattc gctgccctct gtccgttaaa
    20881 atacggccat cttctgtcac tttgtcgccg ttgacgctca tcacaatgac atctttatct
    20941 ttcagcgcgc caataaataa tttttgctgc cactggggga atttatcgct gttatagaag
    21001 gccatgccgc tcacagcggg cgaatctttc cagtaaaaaa caggttgctc ggtcccggcg
    21061 acgatctccc ctttcgcttc cggtatctta aagcctgaat agttgattcc ccaggttgcc
    21121 agcggccagc cgtagttttt gcctttttgc gggatattaa tttcatcacc accgcgcggg
    21181 ccatgttcat tcagccacag tgcattactc cacggattca ttgccattcc ttgcggatta
    21241 cgaatgccat aagaccagat ctcggcgcgc acaccggatt cctttataaa aggattatca
    21301 tccgggattt cgccctggtc ggtcagacgc accagtttgc cctgtaattt atccagatcc
    21361 tgcgccgtcg ggcgctgatt gttttcgccc agagcaataa aaagataacc tttaccgtcg
    21421 aataccagcc gcccgccaaa atggttgccg gtagacagtt ttggcatctg gcggaagacg
    21481 gtgcggaagt cggtcacttt tgagagatca tcacttaagc ggccataacc cacagcagtt
    21541 ccggctttgc catcatcgcc aacttcggaa taacttaacc agatgcggcg agactgagca
    21601 aaatcaggcg ctaaaaccac gtccagcagg ccgccctgcc cgtgcgccca aacgtccgga
    21661 actccggaaa gcggcgcaga taatcctttt cctgcttgcc agtgacgcaa ctcgccgccg
    21721 cgcagagtga ttaacatacc gtgattatcg ggtaaaaagg ccagtgccca gggatggtcg
    21781 agtttgtctt gcagtacttc gacatttacc gttgcaggag ccgcccagag agcggaagaa
    21841 agacaaataa ggggcacaag gaaaaaggat tgtcgatgca taacgcctcc ggattgactc
    21901 tggcttaaag cgtagtcagt ggaggagata acaaattcat ttttacaaaa acttaaacat
    21961 gaagggggag acgctttctc ccccttagtt ttcaggcctt ctcaagcatg gcgtgcttct
    22021 gcaggctctg gatactcagc gttaagctca tcagacaatt ttcaagctta tcggcgttga
    22081 cggtaataac agtcgggcaa tcatggtgcc cactcatcaa acatactgcg gctgtcgcta
    22141 atgcttcttc agcatgatga agagcactcc actcttcctg atccagatga agattcaacc
    22201 gcagcgattt atcgtgcagt tcgcgattca gtttaaaaaa gttatctcgt agatgattgc
    22261 tttcgctgac ggacatgtat ccttttgcct tcctcagttg cacgtatgca gcgaggtcaa
    22321 cgcgtgcgtc gatcaaccgg ttcagcagat cgattcgctg tctgtcaacg aacatacttc
    22381 gatcctcctc tttccagcct tgttgcaatt aaatagtatg gctatttttt gagcaaaatg
    22441 ctgtccagga tcaattcttc acgccgaatt aacaaaattt acatttcctt cgcctccgcc
    22501 agcgaatgag attgcgatac aaattttatc cagaactctg tagaatccct gccctgacct
    22561 ttcctgtaac tgaatatttt ttaagctatg agcaaagtaa ctccccagcc gaaaatcggc
    22621 tttgtttccc ttggctgtcc gaaaaacctt gtcgattcag agcgtattct caccgaacta
    22681 cgcactgaag gttatgacgt ggtaccgagc tatgacgatg cggacatggt gatcgtcaac
    22741 acctgcggct ttattgacag cgcggtacaa gaatcactgg aagccattgg tgaagcgttg
    22801 aatgaaaacg gcaaggtaat tgtgaccggt tgtctggggg caaaagaaga tcagatccgc
    22861 gaagtccacc cgaaagtgct ggaaatcacc gggcctcata gctatgagca ggttctggag
    22921 cacgttcatc actacgtgcc aaaaccgaaa cacaacccat tcctgagcct ggtgccagaa
    22981 caaggtgtga aactgacgcc gcgtcattat gcctatctga aaatttcgga aggctgtaat
    23041 caccgctgca ccttctgcat tattccgtct atgcgcggcg acctggtgag ccgtccgatt
    23101 ggcgaagtgt taagtgaagc gaaacgtctg gtagatgcgg gcgttaaaga gattctggtg
    23161 atctcgcagg atacttctgc ctatggcgtt gatgttaaac atcgtactgg cttccacaac
    23221 ggcgagccgg taaaaaccag catggtcagc ctgtgcgaac agttatcgaa actggggatc
    23281 tggacacgtc tgcactacgt ttacccttat ccgcatgtgg acgacgtcat cccactgatg
    23341 gcagaaggca aaatcctgcc gtatctggac attccgttgc agcacgccag cccgcgcatt
    23401 ctcaaactga tgaagcgtcc gggttctgta gatcgccaac tggcgcgcat caaacagtgg
    23461 cgcgaaatct gcccggaact gaccctacgc tcaaccttta ttgtcggctt ccctggcgag
    23521 acagaagaag atttccagat gctactcgac ttcctgaaag aagcgcgtct ggatcgcgtt
    23581 ggctgcttta aatacagccc ggttgaaggt gcagacgcca atgccctgcc tgaccaggtt
    23641 ccggaagaag ttaaagaaga acgctggaac cgtttcatgc agctgcagca gcagatttcc
    23701 gccgagcgcc tgcaagagaa agtgggccgt gaaattctgg tgattatcga cgaagtggac
    23761 gaagaaggcg cgattggtcg cagcatggca gatgcaccgg aaatcgacgg cgcggtttat
    23821 ctcaatggtg aaaccaacgt taagccgggt gatatcctgc gtgtgaaagt cgagcacgcc
    23881 gatgagtacg atttgtgggg tagccgggtt taattaattc aggccgggca aaatcatttg
    23941 tccggcctgc gaataatgat tactgccagc taatctgagc cctgccgttt ttcttactct
    24001 tatacagccg ctcatccgcc agcattttca agcgttcgac ggtggtacat tcatttgaat
    24061 gcgctacacc gatactggta cttaaattaa tgcttcccac ctggaaatga acgatttctt
    24121 ttagaatcga tagcaactct tccagcgttc cacccgtata aatcaccgca aattcgtcac
    24181 cgccgaagcg gtagatttta cctttatcta ccgggatctg tgatttaagg attttcacca
    24241 ctttcatcaa cacttcatcg cccagcaaat gaccgaaggt gtcgttaatg tttttgaatt
    24301 tatcgacgtc aaacaggcac aaataaccgc ctttttccgg cagcgctttt aaatcttcgt
    24361 caaaggattt tctgcccatt gctttggtca ggtcgtccag gaacgccgag ttatacatac
    24421 tgtagagttt tttgacatcg gcagccgaaa agaaaagccc gccatccagt acgcgttcaa
    24481 aatggttatt aatgatggct tctttctcca caataccacg attaatcact ctgcgtacca
    24541 gatagaggca actggtaccc gatatcactg ccaccaatgc ggcaaagaaa ataatcaatg
    24601 tacagacccg caaataaaca tcttttttat acgcaaatac gtacaatccg gtcggcaaat
    24661 aggcgaagtg ctccatcgct tccgaaacgg tgaagatggc gtttttagca taatattcat
    24721 caccggtcac cggtttaccg ttcttaaata gagagaacat caggttctcg ctgtccatcg
    24781 ccagcgattt tgacagtaaa gaaaaacgct ccagaccaag caaaaatatc agactacgat
    24841 tgtgagttac cggatagagg taatagacga aggttttacc gtcaaaagag ataaaccctg
    24901 tatttttgac atgtcgatac ttcacaacct gcataatatc tgcacaataa tttttctcga
    24961 actcctttac ctgaatacat tcgtcactgg aaatcagata aggaccgaca tcgtacaaat
    25021 aagaggggga aaccgagtcg gcgtaagctg tgcgcaaacc aaccaacagc tgttcctccg
    25081 tgggatttag cgtgttgata agatcaattg ccacgctacg ccgatattca gcaatatttg
    25141 ccaccgcaaa agca
//
LOCUS       DAZKHF020000045        25127 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2201S31241
            SAMN43267800-rid25106413.denovo.045, whole genome shotgun sequence.
ACCESSION   DAZKHF020000045 DAZKHF020000000
VERSION     DAZKHF020000045.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267800
            Sequence Read Archive: SRR30307263
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 25127)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25127)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 25127)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350581.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350581.2
            Genome Coverage       :: 151x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:21:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,652
            CDSs (total)                      :: 5,557
            Genes (coding)                    :: 5,320
            CDSs (with protein)               :: 5,320
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 237
            CDSs (without protein)            :: 237
            Pseudo Genes (ambiguous residues) :: 0 of 237
            Pseudo Genes (frameshifted)       :: 102 of 237
            Pseudo Genes (incomplete)         :: 130 of 237
            Pseudo Genes (internal stop)      :: 52 of 237
            Pseudo Genes (multiple problems)  :: 41 of 237
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25127
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267800-rid25106413.denovo.045"
                     /serotype="O 26:H11"
                     /isolate="2201S31241"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-01"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            complement(102..1766)
                     /gene="glnS"
                     /locus_tag="ACHOSC_RS07415"
                     /old_locus_tag="pgaptmp_001483"
     CDS             complement(102..1766)
                     /gene="glnS"
                     /locus_tag="ACHOSC_RS07415"
                     /old_locus_tag="pgaptmp_001483"
                     /EC_number="6.1.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004819 - glutamine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006425 - glutaminyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--tRNA ligase"
                     /protein_id="HGU4547457.1"
                     /translation="MSEAEARPTNFIRQIIDEDLASGKHTTVHTRFPPEPNGYLHIGH
                     AKSICLNFGIAQDYKGQCNLRFDDTNPVKEDIEYVESIKNDVEWLGFHWSGNVRYSSD
                     YFDQLHAYAIELINKGLAYVDELTPEQIREYRGTLTQPGKNSPYRDRSVEENLALFEK
                     MRAGGFEEGKACLRAKIDMASPFIVMRDPVLYRIKFAEHHQTGNKWCIYPMYDFTHCI
                     SDALEGITHSLCTLEFQDNRRLYDWVLDNITIPVHPRQYEFSRLNLEYTVMSKRKLNL
                     LVTDKHVEGWDDPRMPTISGLRRRGYTAASIREFCKRIGVTKQDNTIEMASLESCIRE
                     DLNENAPRAMAVIDPVKLVIENYQGEGEMVTMPNHPNKPEMGSRQVPFSGEIWIDRAD
                     FREEANKQYKRLVLGKEVRLRNAYVIKAERVEKDAEGNITTIFCTYDADTLSKDPADG
                     RKVKGVIHWVSAAHALPVEIRLYDRLFSVPNPGAADDFLSVINPESLVIKQGFAEPSL
                     KDAVAGKAFQFEREGYFCLDSRHSTAEKPVFNRTVGLRDTWAKVGE"
     gene            complement(1969..3915)
                     /gene="nagE"
                     /locus_tag="ACHOSC_RS07420"
                     /old_locus_tag="pgaptmp_001484"
     CDS             complement(1969..3915)
                     /gene="nagE"
                     /locus_tag="ACHOSC_RS07420"
                     /old_locus_tag="pgaptmp_001484"
                     /EC_number="2.7.1.193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_function: GO:0015572 - N-acetylglucosamine
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS N-acetyl glucosamine transporter subunit
                     IIABC"
                     /protein_id="HGU4547458.1"
                     /translation="MNILGFFQRLGRALQLPIAVLPVAALLLRFGQPDLLNVAFIAQA
                     GGAIFDNLALIFAIGVASSWSKDSAGAAALAGAVGYFVLTKAMVTINPEINMGVLAGI
                     ITGLVGGAAYNRWSDIKLPDFLSFFGGKRFVPIATGFFCLVLAAIFGYVWPPVQHAIH
                     AGGEWIVSAGALGSGIFGFINRLLIPTGLHQVLNTIAWFQIGEFTNAAGTVFHGDINR
                     FYAGDGTAGMFMSGFFPIMMFGLPGAALAMYFAAPKERRPMVGGMLLSVAVTAFLTGV
                     TEPLEFLFMFLAPLLYLLHALLTGISLFVATLLGIHAGFSFSAGAIDYALMYNLPAAS
                     QNVWMLLVMGVVFFAIYFVVFSLVIRMFNLKTPGREDKEDEIVTEEANSNTEEGLNQL
                     ATNYIAAVGGTDNLKAIDACITRLRLTVADSARVNDTMCKRLGASGVVKLNKQTIQVI
                     VGAKAESIGDAMKKVVARGPVAAASAEATPATAAPVAKPQAVPNAVSIAELVSPITGD
                     VVALDQVPDEAFASKAVGDGVAVKPTDKIVVSPAAGTIVKIFNTNHAFCLETEKGAEI
                     VVHMGIDTVALEGKGFKRLVEEGAQVSAGQPILEMDLDYLNANARSMISPVVCSNIDD
                     FSGLIIKAQGHVVAGQTPLYEIKK"
     gene            4248..5048
                     /gene="nagB"
                     /locus_tag="ACHOSC_RS07425"
                     /old_locus_tag="pgaptmp_001485"
     CDS             4248..5048
                     /gene="nagB"
                     /locus_tag="ACHOSC_RS07425"
                     /old_locus_tag="pgaptmp_001485"
                     /EC_number="3.5.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008805656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004342 - glucosamine-6-phosphate
                     deaminase activity [Evidence IEA];
                     GO_process: GO:0006044 - N-acetylglucosamine metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucosamine-6-phosphate deaminase"
                     /protein_id="HGU4547459.1"
                     /translation="MRLIPLTTAEQVGKWAARHIVNRINAFKPTADRPFVLGLPTGGT
                     PMTTYKALVEMHKAGQVSFKHVVTFNMDEYVGLPKEHPESYYSFMHRNFFDHVDIPAE
                     NINLLNGNAPDIDAECRQYEEKIRSYGKIHLFMGGVGNDGHIAFNEPASSLASRTRIK
                     TLTHDTRVANSRFFDNDVNQVPKYALTVGVGTLLDAEEVMILVLGSQKALALQAAVEG
                     CVNHMWTISCLQLHPKAIMVCDEPSTMELKVKTLRYFNELEAENIKGL"
     gene            5108..6256
                     /gene="nagA"
                     /locus_tag="ACHOSC_RS07430"
                     /old_locus_tag="pgaptmp_001486"
     CDS             5108..6256
                     /gene="nagA"
                     /locus_tag="ACHOSC_RS07430"
                     /old_locus_tag="pgaptmp_001486"
                     /EC_number="3.5.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008448 - N-acetylglucosamine-6-phosphate
                     deacetylase activity [Evidence IEA];
                     GO_process: GO:0006044 - N-acetylglucosamine metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylglucosamine-6-phosphate deacetylase"
                     /protein_id="HGU4547460.1"
                     /translation="MYALTQGRIFTGHEFLDDHAVVIADGLIKSVCPVAELPPEIEQR
                     SLNGAILSPGFIDVQLNGCGGVQFNDTAEAVSVETLEIMQKANEKSGCTNYLPTLITT
                     SDELMKQGVRVMREYLAKHPNQALGLHLEGPWLNLVKKGTHNPNFVRKPDAALVDFLC
                     ENADVITKVTLAPEMVPAEVISKLANAGIVVSAGHSNATLKEAKAGFRAGITFATHLY
                     NAMPYITGREPGLAGAILDEADIYCGIIADGLHVDYANIRNAKRLKGDKLCLVTDATA
                     PAGANIEQFIFAGKTIYYRNGLCVDENGTLSGSSLTMIEGVRNLVEHCGIALDEVLRM
                     ATLYPARAIGVEKRLGTLAAGKVANLTAFTPDFKITKTIVNGNEVVTQ"
     gene            6265..7485
                     /gene="nagC"
                     /locus_tag="ACHOSC_RS07435"
                     /old_locus_tag="pgaptmp_001487"
                     /gene_synonym="nagR"
     CDS             6265..7485
                     /gene="nagC"
                     /locus_tag="ACHOSC_RS07435"
                     /old_locus_tag="pgaptmp_001487"
                     /gene_synonym="nagR"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator NagC"
                     /protein_id="HGU4547461.1"
                     /translation="MTPGGQAQIGNVDLVKQLNSAAVYRLIDQYGPISRIQIAEQSQL
                     APASVTKITRQLIERGLIKEVDQQASTGGRRAISIVTETRNFHAIGVRLGRHDATITL
                     FDLSSKVLAEEHYPLPERTQQTLEHALLNAIAQFIDSYQRKLRELIAISVILPGLVDP
                     DSGKIHYMPHIQVENWGLVEALEERFKVTCFVGHDIRSLALAEHYFGASQDCEDSILV
                     RVHRGTGAGIISNGRIFIGRNGNVGEIGHIQVEPLGERCHCGNFGCLETIAANAAIEQ
                     RVLNLLKQGYQSRVPLDDCTIKTICKAANKGDSLASEVIEYVGRHLGKTIAIAINLFN
                     PQKIVIAGEITEADKVLLPAIESCINTQALKAFRTNLPVVRSELDHRSAIGAFALVKR
                     AMLNGILLQHLLEN"
     gene            7533..8285
                     /gene="nagD"
                     /locus_tag="ACHOSC_RS07440"
                     /old_locus_tag="pgaptmp_001488"
     CDS             7533..8285
                     /gene="nagD"
                     /locus_tag="ACHOSC_RS07440"
                     /old_locus_tag="pgaptmp_001488"
                     /EC_number="3.1.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonucleotide monophosphatase NagD"
                     /protein_id="HGU4547462.1"
                     /translation="MTIKNVICDIDGVLMHDNVAVPGAAEFLHGIMDKGLPLVLLTNY
                     PSQTGQDLANRFATAGVDVPDSVFYTSAMATADFLRRQEGKKAYVVGEGALIHELYKA
                     GFTITDVNPDFVIVGETRSYNWDMMHKAAYFVANGARFIATNPDTHGRGFYPACGALC
                     AGIEKISGRKPFYVGKPSPWIIRAALNKMQAHSEETVIVGDNLRTDILAGFQAGLETI
                     LVLSGVSSLDDIDSMPFRPSWIYPSVAEIDVI"
     gene            8542..10206
                     /gene="asnB"
                     /locus_tag="ACHOSC_RS07445"
                     /old_locus_tag="pgaptmp_001489"
     CDS             8542..10206
                     /gene="asnB"
                     /locus_tag="ACHOSC_RS07445"
                     /old_locus_tag="pgaptmp_001489"
                     /EC_number="6.3.5.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001724523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004066 - asparagine synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006529 - asparagine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine synthase B"
                     /protein_id="HGU4547463.1"
                     /translation="MCSIFGVFDIKTDAVELRKKALELSRLMRHRGPDWSGIYASDNA
                     ILAHERLSIVDVNAGAQPLYNQQKTHVLAVNGEIYNHQALRAEYGDRYQFQTGSDCEV
                     ILALYQEKGPEFLDDLQGMFAFALYDSEKDAYLIGRDHLGIIPLYMGYDEHGQLYVAS
                     EMKALVPVCRTIKEFPAGSYLWSQDGEIRSYYHRDWFDFDAVKDNVTDKNELRQALED
                     SVKSHLMSDVPYGVLLSGGLDSSIISAITKKYAARRVEDQERSEAWWPQLHSFAVGLP
                     GSPDLKAAQEVANHLGTVHHEIHFTVQEGLDAIRDVIYHIETYDVTTIRASTPMYLMS
                     RKIKAMGIKMVLSGEGSDEVFGGYLYFHKAPNAKELHEETVRKLLALHMYDCARANKA
                     MSAWGVEARVPFLDKKFLDVAMRINPQDKMCGNGKMEKHILRECFESYLPASVAWRQK
                     EQFSDGVGYSWIDTLKEVAAQQVSDQQLETARFRFPYNTPTSKEAYLYREIFEELFPL
                     PSAAECVPGGPSVACSSAKAIEWDEAFKKMDDPSGRAVGVHQSAYK"
     gene            10587..10663
                     /locus_tag="ACHOSC_RS07450"
                     /old_locus_tag="pgaptmp_001490"
     tRNA            10587..10663
                     /locus_tag="ACHOSC_RS07450"
                     /old_locus_tag="pgaptmp_001490"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:10621..10623,aa:Met,seq:cat)
     gene            10672..10756
                     /locus_tag="ACHOSC_RS07455"
                     /old_locus_tag="pgaptmp_001491"
     tRNA            10672..10756
                     /locus_tag="ACHOSC_RS07455"
                     /old_locus_tag="pgaptmp_001491"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:10706..10708,aa:Leu,seq:tag)
     gene            10780..10854
                     /locus_tag="ACHOSC_RS07460"
                     /old_locus_tag="pgaptmp_001492"
     tRNA            10780..10854
                     /locus_tag="ACHOSC_RS07460"
                     /old_locus_tag="pgaptmp_001492"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:10812..10814,aa:Gln,seq:ttg)
     gene            10889..10963
                     /locus_tag="ACHOSC_RS07465"
                     /old_locus_tag="pgaptmp_001493"
     tRNA            10889..10963
                     /locus_tag="ACHOSC_RS07465"
                     /old_locus_tag="pgaptmp_001493"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:10921..10923,aa:Gln,seq:ttg)
     gene            10979..11055
                     /locus_tag="ACHOSC_RS07470"
                     /old_locus_tag="pgaptmp_001494"
     tRNA            10979..11055
                     /locus_tag="ACHOSC_RS07470"
                     /old_locus_tag="pgaptmp_001494"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:11013..11015,aa:Met,seq:cat)
     gene            11104..11178
                     /locus_tag="ACHOSC_RS07475"
                     /old_locus_tag="pgaptmp_001495"
     tRNA            11104..11178
                     /locus_tag="ACHOSC_RS07475"
                     /old_locus_tag="pgaptmp_001495"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:11136..11138,aa:Gln,seq:ctg)
     gene            11216..11290
                     /locus_tag="ACHOSC_RS07480"
                     /old_locus_tag="pgaptmp_001496"
     tRNA            11216..11290
                     /locus_tag="ACHOSC_RS07480"
                     /old_locus_tag="pgaptmp_001496"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:11248..11250,aa:Gln,seq:ctg)
     gene            complement(11444..12619)
                     /gene="ubiF"
                     /locus_tag="ACHOSC_RS07485"
                     /old_locus_tag="pgaptmp_001497"
     CDS             complement(11444..12619)
                     /gene="ubiF"
                     /locus_tag="ACHOSC_RS07485"
                     /old_locus_tag="pgaptmp_001497"
                     /EC_number="1.14.99.60"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706550.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016709 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen, NAD(P)H as one donor, and incorporation
                     of one atom of oxygen [Evidence IEA];
                     GO_process: GO:0006744 - ubiquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-demethoxyubiquinol 3-hydroxylase"
                     /protein_id="HGU4547464.1"
                     /translation="MTNQPTEIAIVGGGMVGGALALGLAQHGFSVTVIEHAEPAPFVA
                     DSQPDVRISAISAASVSLLKGLGVWDAVQAMRCHPYRRLETWEWETAHVMFDAAELKL
                     PLLGYMVENTVLQQALWQALEAHPKVTLRVPGSLIALHRDNDLQELELKGGEVIRAKL
                     VIGADGANSQVRQMAGIGVHAWQYAQSCMLISVQCENDPGDSTWQQFTPDGPRAFLPL
                     FDNWASLVWYDSPVRIRQLQNMNMAQLQAEIAKHFPSRLGYVTPLAAGAFPLTRRHAL
                     QYVQPGLALVGDAAHTIHPLAGQGGNLGYRDVDALIDVLVNARSYGEAWASYPVLKRY
                     QMRRMADNFIMQSGMDLFYAGFSNNLPPLRFVRNLGLMAAERAGVLKRQALKYALGL"
     gene            12765..14189
                     /gene="miaB"
                     /locus_tag="ACHOSC_RS07490"
                     /old_locus_tag="pgaptmp_001498"
     CDS             12765..14189
                     /gene="miaB"
                     /locus_tag="ACHOSC_RS07490"
                     /old_locus_tag="pgaptmp_001498"
                     /EC_number="2.8.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016782 - transferase activity,
                     transferring sulphur-containing groups [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (N6-isopentenyl
                     adenosine(37)-C2)-methylthiotransferase MiaB"
                     /protein_id="HGU4547465.1"
                     /translation="MTKKLHIKTWGCQMNEYDSSKMADLLDATHGYQLTDVAEEADVL
                     LLNTCSIREKAQEKVFHQLGRWKLLKEKNPDLIIGVGGCVASQEGEHIRQRAHYVDII
                     FGPQTLHRLPEMINSVRGDRSPVVDISFPEIEKFDRLPEPRAEGPTAFVSIMEGCNKY
                     CTYCVVPYTRGEEVSRPSDDILFEIAQLAAQGVREVNLLGQNVNAWRGENYDGTTGSF
                     ADLLRLVAAIDGIDRIRFTTSHPIEFTDDIIEVYRDTPELVSFLHLPVQSGSDRILNL
                     MGRTHTALEYKAIIRKLRAARPDIQISSDFIVGFPGETTEDFEKTMKLIADVNFDMSY
                     SFIFSARPGTPAADMVDDVPEEEKKQRLYILQERINQQAMAWSRRMLGTTQRILVEGT
                     SRKSIMELSGRTENNRVVNFEGTPDMIGKFVDVEITDVYPNSLRGKVVRTEDEMGLRV
                     AETPESVIARTRKENDLGVGYYQP"
     gene            14342..15382
                     /locus_tag="ACHOSC_RS07495"
                     /old_locus_tag="pgaptmp_001499"
     CDS             14342..15382
                     /locus_tag="ACHOSC_RS07495"
                     /old_locus_tag="pgaptmp_001499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415193.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PhoH family protein"
                     /protein_id="HGU4547466.1"
                     /translation="MNIDTREITLEPADNARLLSLCGPFDDNIKQLERRLGIEINRRD
                     NHFKLTGRPICVTAAADILRSLYVDTAPMRGQIQDIEPEQIHLAIKEARVLEQSAESV
                     PEYGKAVNIKTKRGVIKPRTPNQAQYIANILDHDITFGVGPAGTGKTYLAVAAAVDAL
                     ERQEIRRILLTRPAVEAGEKLGFLPGDLSQKVDPYLRPLYDALFEMLGFEKVEKLIER
                     NVIEVAPLAYMRGRTLNDAFIILDESQNTTIEQMKMFLTRIGFNSKAVITGDVTQIDL
                     PRNTKSGLRHAIEVLADVEEISFNFFHSEDVVRHPVVARIVNAYEAWEEAEQKRKAAL
                     AAERKREEQEQK"
     gene            15379..15846
                     /gene="ybeY"
                     /locus_tag="ACHOSC_RS07500"
                     /old_locus_tag="pgaptmp_001500"
     CDS             15379..15846
                     /gene="ybeY"
                     /locus_tag="ACHOSC_RS07500"
                     /old_locus_tag="pgaptmp_001500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014169133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004540 - RNA nuclease activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA maturation RNase YbeY"
                     /protein_id="HGU4547467.1"
                     /translation="MSQVILDLQLACEDNSGLPEESQFQTWLNAVIPQFQEESEVTIR
                     VVDTAESHSLNLTYRGKDKPTNVLSFPFEVPPGMEMSLLGDLVICRQVVEKEAQEQGK
                     PLEAHWAHMVVHGSLHLLGYDHIEDDEAEEMEALETEIMLALGYEDPYIAEKE"
     gene            15936..16814
                     /gene="corC"
                     /locus_tag="ACHOSC_RS07505"
                     /old_locus_tag="pgaptmp_001501"
     CDS             15936..16814
                     /gene="corC"
                     /locus_tag="ACHOSC_RS07505"
                     /old_locus_tag="pgaptmp_001501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308723.1"
                     /note="CorC(YbeX) belongs to the Cyclin M Mg2+ Exporter
                     (CNNM) family, and was characterized as belonging to a set
                     of three proteins, at least one of which must be present
                     for CorA to function; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CNNM family magnesium/cobalt transport protein
                     CorC"
                     /protein_id="HGU4547468.1"
                     /translation="MSDDNSHSSDTISNKKGFFSLLLSQLFHGEPKNRDELLALIRDS
                     GQNDLIDEDTRDMLEGVMDIADQRVRDIMIPRSQMITLKRNQTLDECLDVIIESAHSR
                     FPVISEDKDHIEGILMAKDLLPFMRSDAEAFSMDKVLRQAVVVPESKRVDRMLKEFRS
                     QRYHMAIVIDEFGGVSGLVTIEDILELIVGEIEDEYDEEDDIDFRQLSRHTWTVRALA
                     SIEDFNEAFGTHFSDEEVDTIGGLVMQAFGHLPARGETIDIDGYQFKVAMADSRRIIQ
                     VHVKIPDDSPQPKLDE"
     gene            16839..18377
                     /gene="lnt"
                     /locus_tag="ACHOSC_RS07510"
                     /old_locus_tag="pgaptmp_001502"
     CDS             16839..18377
                     /gene="lnt"
                     /locus_tag="ACHOSC_RS07510"
                     /old_locus_tag="pgaptmp_001502"
                     /EC_number="2.3.1.269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016410 - N-acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0042158 - lipoprotein biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="apolipoprotein N-acyltransferase"
                     /protein_id="HGU4547469.1"
                     /translation="MAFASLIERQRIRLLLALLFGACGTLAFSPYDVWPAAIVSLMGL
                     QALTFNRRPLQSAAIGFCWGFGLFGTGINWVYVSIATFGGMPGPVNIFLVVLLAAYLS
                     LYTGLFAGVLSRLWPKTTWLRVAIAAPALWQVTEFLRGWVLTGFPWLQFGYSQIDGPL
                     KGLAPIMGVEAINFLLMMVSGLLALALVKRNWRPLVVAVVLFALPFPLRYIQWFTPQP
                     EKTIQVSMVQGDIPQSLKWDGDQLLNTLKIYYNATAPLLGKSSLIIWPESAITDLEIN
                     QQPFLKALDGELRDKGSSLVTGIVDARLNKQNRYDTYNTIITLGKGAPYSYESADRYN
                     KNHLVPFGEFVPLESILRPLAPFFDLPMSSFSRGPYIQPPLSVNGIELTAAICYEIIL
                     GEQVRDNFRPDTDYLLTISNDAWFGKSIGPWQHFQMARMRALELARPLLRSTNNGITA
                     VIGPQGEIQAMIPQFTREVLTTNVTPTTGLTPYARTGNWPLWVLTALFGFAAVLMSLR
                     ARKR"
     gene            complement(18455..20332)
                     /locus_tag="ACHOSC_RS07515"
                     /old_locus_tag="pgaptmp_001503"
     CDS             complement(18455..20332)
                     /locus_tag="ACHOSC_RS07515"
                     /old_locus_tag="pgaptmp_001503"
                     /EC_number="3.4.21.105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001276742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease"
                     /protein_id="HGU4547470.1"
                     /translation="MSASSVKPLNVQLPAITLILFALCIGIFCYLAQWMSYEEVDQSA
                     LIHLGANVAPLTLSGEPWRLLSSIFLHSSVSHLLMNMFAFLVVGGVAEQILGKWRLLI
                     TWLFSGVFGGLISACYALRESEQIVISVGASGAILGIAGAAIATQFASGTGTYHKNQR
                     RVFPLLGMVALTLLYGARQTGIDNACHIGGLIAGGALGWLSARLSGQNRLVTEGGIIV
                     AGSLLLTGAIWLAQQQMDESVLQVRQSLREAFYPQEIEQERRQKKQQLAEERNALRET
                     LSAPVSREQASGDLLAEIADIHDMAISRDGNTLYAAIENTNSIVVFDLGQKKILHTFT
                     APIAKEKSVKHCGGCKDQGVRSLALSPDEKLIYATSFEANALSVINVATGEIIQSITT
                     GAHPDSFILSRDGTKAWVMNRTSNSVSAIDLVTYQHVADIPLEKYDGTGTSGKPGAWV
                     MALSPDERTLLVPGAGRGNIVRINTITHQKEDFPAGDARGTISAMRFRPENGEVIFAD
                     SQGISRISVGDQQASIMTQWCSRSVYSVEGISPDGQYLALVSYGLQGYVILLNINAGQ
                     IIGVYPASYVNHLRFSADGRKIFVMAKNGLIQMDRTRSLDPQAIIRHPQYGNVACIPE
                     P"
     gene            20787..21695
                     /gene="gltI"
                     /locus_tag="ACHOSC_RS07520"
                     /old_locus_tag="pgaptmp_001504"
     CDS             20787..21695
                     /gene="gltI"
                     /locus_tag="ACHOSC_RS07520"
                     /old_locus_tag="pgaptmp_001504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate/aspartate ABC transporter
                     substrate-binding protein GltI"
                     /protein_id="HGU4547471.1"
                     /translation="MQLRKPATAILALALSAGLAQADDAAPVAGSTLDKIAKNGVIVV
                     GHRESSVPFSYYDNQQKVVGYSQDYSNAIVEAVKKKLNKPDLQVKLIPITSQNRIPLL
                     QNGTFDFECGSTTNNVERQKQAAFSDTIFVVGTRLLTKKGGDIKDFADLKGKAVVVTS
                     GTTSEVLLNKLNEEQKMNMRIISAKDHGDSFRTLESGRAVAFMMDDALLAGERAKAKK
                     PDNWDIVGKPQSQEAYGCMLRKDDPQFKKLMDDTIAQVQTSGEAEKWFDKWFKNPIPP
                     KNLNMNFELSDEMKALFKEPNDKALN"
     gene            21865..22605
                     /gene="gltJ"
                     /locus_tag="ACHOSC_RS07525"
                     /old_locus_tag="pgaptmp_001505"
     CDS             21865..22605
                     /gene="gltJ"
                     /locus_tag="ACHOSC_RS07525"
                     /old_locus_tag="pgaptmp_001505"
                     /EC_number="7.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate/aspartate ABC transporter permease
                     GltJ"
                     /protein_id="HGU4547472.1"
                     /translation="MSIDWNWGIFLQQAPFGNTTYLGWIWSGFQVTIALSICAWIIAF
                     LVGSFFGILRTVPNRFLSGLGTLYVELFRNVPLIVQFFTWYLVIPELLPEKIGMWFKA
                     ELDPNIQFFLSSMLCLGLFTAARVCEQVRAAIQSLPRGQKNAALAMGLTLPQAYRYVL
                     LPNAYRVIVPPMTSEMMNLVKNSAIASTIGLVDMAAQAGKLLDYSAHAWESFTAITLA
                     YVLINAVIMLVMTLVERKVRLPGNMGGK"
     gene            22605..23279
                     /gene="gltK"
                     /locus_tag="ACHOSC_RS07530"
                     /old_locus_tag="pgaptmp_001506"
     CDS             22605..23279
                     /gene="gltK"
                     /locus_tag="ACHOSC_RS07530"
                     /old_locus_tag="pgaptmp_001506"
                     /EC_number="7.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate/aspartate ABC transporter permease
                     GltK"
                     /protein_id="HGU4547473.1"
                     /translation="MYEFDWSSIVPSLPYLLDGLVITLKITVTAVVIGILWGTMLAVM
                     RLSSFAPVAWFAKAYVNVFRSIPLVMVLLWFYLIVPGFLQNVLGLSPKNDIRLISAMV
                     AFSMFEAAYYSEIIRAGIQSISRGQSSAALALGMTHWQSMKLIILPQAFRAMVPLLLT
                     QGIVLFQDTSLVYVLSLADFFRTASTIGERDGTQVEMILFAGFVYFVISLSASLLVSY
                     LKRRTA"
     gene            23279..24004
                     /gene="gltL"
                     /locus_tag="ACHOSC_RS07535"
                     /old_locus_tag="pgaptmp_001507"
     CDS             23279..24004
                     /gene="gltL"
                     /locus_tag="ACHOSC_RS07535"
                     /old_locus_tag="pgaptmp_001507"
                     /EC_number="7.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020245804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate/aspartate ABC transporter ATP-binding
                     protein GltL"
                     /protein_id="HGU4547474.1"
                     /translation="MITLKNVSKWYGHFQVLTDCSTEVKKGEVVVVCGPSGSGKSTLI
                     KTVNGLEPVQQGEITVDGIVVNDKKTDLAKLRSRVGMVFQHFELFPHLSIIENLTLAQ
                     VKVLKRDKAPAREKALKLLERVGLSAHANKFPAQLSGGQQQRVAIARALCMDPIAMLF
                     DEPTSALDPEMINEVLDVMVELANEGMTMMVVTHEMGFARKVANRVIFMDEGKIVEDS
                     PKDAFFDDPKSDRAKDFLAKILH"
     gene            24122..25057
                     /gene="rihA"
                     /locus_tag="ACHOSC_RS07540"
                     /old_locus_tag="pgaptmp_001508"
     CDS             24122..25057
                     /gene="rihA"
                     /locus_tag="ACHOSC_RS07540"
                     /old_locus_tag="pgaptmp_001508"
                     /EC_number="3.2.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050263 - ribosylpyrimidine nucleosidase
                     activity [Evidence IEA];
                     GO_process: GO:0006206 - pyrimidine nucleobase metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine-specific ribonucleoside hydrolase
                     RihA"
                     /protein_id="HGU4547475.1"
                     /translation="MALPILLDCDPGHDDAIAIVLALASPELDVKAITSSAGNQTPEK
                     TLRNVLRMLTLLNRTDIPVAGGAVKPLMRELIIADNVHGESGLDGPALPEPTFAPQNC
                     TAVELMAKTLRESAEPVTIVSTGPQTNVALLLNSHPELHSKIARIVIMGGAMGLGNWT
                     PAAEFNIYVDPEAAEIVFQSGIPVVMAGLDVTHKAQIHVEDTERFRAIGNPVSTIVAE
                     LLDFFLEYHKDEKWGFVGAPLHDPCTIAWLLKPELFTTVERWVGVETQGKYSQGMTVV
                     DYYYLTGNKPNATVMVDVDRQGFVDLLADRLKFYA"
BASE COUNT         6124 a         6571 c         6417 g         6015 t
ORIGIN      
        1 gaaaagcaga aaaaacgccg cagagtgcgg cgtttgggat tgtttgctat ttcttgccgg
       61 atgcgacgta aatgcctcac ccggcctggt agaacttaaa attactcgcc tactttcgcc
      121 caggtatcac gcagcccaac ggtgcggtta aataccggtt tttccgctgt ggaatggcgg
      181 ctgtcgaggc agaagtaacc ttcacgctca aactggaatg ctttacccgc aaccgcatct
      241 ttcagcgacg gttcagcaaa gccctgtttg atcaccagcg attccgggtt aatcaccgac
      301 aggaaatcat ccgcagcacc agggttcggc acgctgaaca gacggtcgta cagacggatt
      361 tcaaccggca gcgcatgtgc cgcgctcacc cagtgaataa cacctttgac tttacgacca
      421 tctgccggat ctttgcttaa ggtgtcggcg tcataagtac agaagatggt ggtgatattg
      481 ccttcggcat ctttctccac gcgttcagcc ttaatgacgt aagcattacg cagacgcact
      541 tctttaccca gcaccagacg tttgtactgc ttgttagctt cttcgcggaa atcggcgcga
      601 tcgatccaaa tctcaccgct aaacggcacc tgacgactcc ccatttccgg tttgttcgga
      661 tggttcggca tggtgaccat ttcgccttcg ccctgatagt tttcgataac cagtttcacc
      721 ggatcgataa ccgccattgc gcgcggcgca ttttcgttga gatcttcacg gatgcaggat
      781 tccagcgatg ccatctcaat ggtgttgtcc tgcttggtca cgccgatgcg tttgcagaac
      841 tcacgaatag aagcagcagt gtaaccacga cgacgcagac cggaaatggt cggcatacgc
      901 gggtcatccc agccttcaac gtgcttgtcg gtcaccagca ggttcagctt acgcttggac
      961 atcacagtgt attccagatt caggcgcgag aattcgtact ggcgcgggtg aacaggaatg
     1021 gtgatgttgt ccagcaccca gtcgtacagg cgacggttgt cctggaactc aagcgtacac
     1081 agagagtgcg taataccttc cagggcatcg ctgatgcagt gggtgaagtc gtacatcggg
     1141 tagatgcacc acttgttgcc agtctggtgg tgttcagcaa atttaatacg gtacagcacc
     1201 ggatcgcgca tcacgataaa cggtgaagcc atgtcgattt tcgcacgcag gcaggcttta
     1261 ccttcttcaa aaccaccggc acgcattttt tcgaacagcg ccaggttctc ttcaacgctg
     1321 cggtcgcggt acgggctgtt tttacccggt tgcgtcaggg tgccgcggta ttcgcggatc
     1381 tgttccggcg tcagttcatc aacgtacgcc aggcctttat tgatcagttc gatcgcatag
     1441 gcgtggagct gatcaaaata atcggaggag taacggacgt taccagacca gtgaaaacct
     1501 aaccactcta cgtcgttttt gatcgactca acatactcga tatcttcttt taccgggtta
     1561 gtgtcgtcga aacgcaggtt gcactggcct ttatagtcct gggcgatccc gaagttcagg
     1621 cagatagatt tcgcatggcc aatatgcaga tagccattcg gctccggcgg gaaacgggtg
     1681 tgtactgtgg tgtgcttacc actggccaga tcttcatcga tgatctgacg gataaagtta
     1741 gtcgggcggg cttctgcctc actcatcgtg gattcctcaa agcgtaaaca acgtataacg
     1801 gcgtatgatc ttataagcgg gacaggctga caactgttag ttttttaaaa ttgataatct
     1861 aacaaggatt atggggatga ttgatgaaaa aaaaagcggc agagaagatc tccaccgctt
     1921 gtcattgttg gatgcgacgc tcaagcgtcg catcaggcat aaagcagatt actttttgat
     1981 ttcatacagc ggtgtttgac ccgccacaac atggccctga gctttaatga tcaagccact
     2041 gaaatcgtcg atattgctgc aaaccaccgg gctaatcatc gagcgggcgt tagcgttcag
     2101 gtaatccaga tccatttcca gaatcggttg ccctgcgctt acctgcgcac cctcttccac
     2161 cagacgttta aagcctttac cttccagcgc tacggtgtcg atacccatat ggacgacgat
     2221 ctccgcgcct ttttcggttt ccaggcagaa cgcgtggttg gtgttgaaga ttttcacgat
     2281 tgtccctgcg gctggtgata cgacgatttt atctgtcggt ttcaccgcca caccgtcacc
     2341 caccgctttg ctggcgaatg cttcgtcagg aacctgatcc agtgccacga catcaccggt
     2401 aatcggcgat accagctccg cgatagatac cgcgtttggt acagcctgcg gttttgctac
     2461 aggcgcggca gttgccggag ttgcttcagc tgacgcagcg gctaccggac cacgggcaac
     2521 gactttcttc atcgcatcgc cgatggattc tgctttcgcg ccaacaatca cctgaatagt
     2581 ctgtttgttc agtttcacta caccagaagc acccagacgt ttacacatcg tatcgttgac
     2641 gcgggcagag tcagccacgg tcaggcgcag acgggtgata caggcgtcaa ttgctttcag
     2701 gttgtcagtg ccgccaaccg cagcaatata gttggtcgcc agttgattga ggccttcttc
     2761 agtgttgctg ttggcttctt cagtaacgat ctcgtcttct ttatcttcac gacccggcgt
     2821 tttcaggttg aacatgcgga taaccaaact gaacaccacg aagtagatag cgaagaagac
     2881 aacacccata accagcagca tccagacgtt ctggctggcg gccggcaggt tatacatcaa
     2941 cgcgtagtcg atagcccccg ctgagaaaga gaagccagca tggataccca gcagtgttgc
     3001 cacaaacagg ctgataccgg tcagcagtgc gtgcaggagg tacagcagcg gtgcaaggaa
     3061 catgaacagg aattccagcg gctcagtcac accggtcagg aacgcagtaa cagcaacaga
     3121 aagcagcatc ccgccaacca tcggacgacg ctctttcggt gctgcgaagt acatcgccag
     3181 cgccgcaccc ggcagaccga acatcataat cgggaagaag ccggacatga acatccccgc
     3241 ggtgccgtca ccggcataga agcggttgat atcaccgtgg aaaaccgtac ccgccgcgtt
     3301 ggtgaattca ccaatctgga accaggcgat ggtgttcagc acctgatgca gaccggttgg
     3361 gatcagcaga cggttgatga aaccaaagat accggaaccc agcgcgcccg cagaaacgat
     3421 ccactcgcct cctgcatgga tagcgtgctg taccggcggc cagacgtaac caaaaatggc
     3481 cgccagcacc aggcagaaga agccggtggc aatcggcaca aagcgtttgc cgccgaagaa
     3541 actcaggaag tccggcagtt taatatcgga ccaacggtta taggctgcgc caccaaccag
     3601 accggtaatg atacccgcca gtacacccat gttaatttct gggttgatgg tcaccatcgc
     3661 tttggttaac acaaagtaac ctaccgcacc cgccagcgcc gccgcacctg cgctgtcttt
     3721 cgaccagctg gatgccacac cgatggcgaa gattaatgcg aggttatcaa aaatcgcacc
     3781 gcccgcctgg gcaataaacg caacgttaag taaatctggc tgaccgaatc gcagcaacag
     3841 tgccgccacc ggcagcaccg cgatagggag ctgtaacgcc ctaccgagtc gctggaaaaa
     3901 acctaaaata ttcatcttat tccccctacg agaaccctat ttggctcgtt taaagccgta
     3961 tttttatttt gctgcaaatt gtactgccga tgttctgtaa tcagattgtt agatcatctg
     4021 ctacagagtg tgtgaaaatt taattcgtat cgcaaattaa acgcgtgtct tttgtgagtt
     4081 ttgtcaccaa atatcgttat catcactccc ttttactggc taaagcagaa aacttatttt
     4141 atcattcaaa aaatcaggtc ggattgacgc ctgtctgcgc aaatccaggt tacgcttaaa
     4201 gatgcctaat ccgccaacgg cttacatttt acttattgag gtgaataatg agactgatcc
     4261 ccctgactac cgctgaacag gtcggcaaat gggctgctcg ccatatcgtc aatcgtatca
     4321 atgcgttcaa accgactgcc gatcgtccgt ttgtactggg cctgccgact ggcggcacgc
     4381 cgatgaccac ctataaagcg ttagtcgaaa tgcataaagc aggccaggtc agctttaagc
     4441 acgttgtcac cttcaacatg gacgaatatg tcggtctgcc gaaagagcat ccggaaagct
     4501 actacagctt tatgcaccgt aatttcttcg atcacgttga tattccagca gaaaacatca
     4561 accttctcaa cggcaatgcc ccggatatcg acgccgagtg ccgccagtat gaagaaaaaa
     4621 tccgttctta cggaaaaatt catctgttta tgggcggtgt aggtaacgac ggtcatattg
     4681 catttaacga accggcgtct tctctggctt ctcgtactcg tatcaaaacc ctgactcatg
     4741 acactcgcgt cgcgaactct cgtttctttg ataacgatgt caatcaggtg ccaaaatatg
     4801 ccctgactgt cggtgttggt acattgctgg atgccgaaga agtgatgatt ctggtgctgg
     4861 gtagccagaa agcactggca ctgcaggctg cagttgaagg ttgcgtgaac cacatgtgga
     4921 ccatcagctg tctgcaactg catccaaaag cgatcatggt gtgcgatgaa ccttccacca
     4981 tggagctgaa agttaagact ttaagatatt tcaatgaatt agaagcagaa aatatcaaag
     5041 gtctgtaatt gttatccctg ccctggctcc ttgctcaggg caatattttt taaaatcggg
     5101 ggtcagaatg tatgcattaa cccagggccg gatctttacc ggccacgaat ttcttgatga
     5161 ccacgcggtt gttatcgctg atggcctgat taaaagcgtc tgtccggtag cggaactgcc
     5221 gccagagatc gaacaacgtt cactgaacgg ggccattctc tcccccggtt ttatcgatgt
     5281 gcagttaaac ggctgcggcg gcgtacagtt taacgacacc gctgaagcgg tcagcgtgga
     5341 aacgctggaa atcatgcaga aagccaatga gaaatcaggc tgtactaact atctgccgac
     5401 gcttatcacc accagcgatg agctgatgaa acagggcgtg cgcgttatgc gcgagtacct
     5461 ggcaaaacat ccgaatcagg cgttaggtct gcatctggaa ggtccgtggc tgaatctggt
     5521 aaaaaaaggc acccataatc cgaattttgt gcgtaagcct gatgccgcgc tggtcgattt
     5581 cctgtgtgaa aacgccgacg tcattaccaa agtgaccctg gcaccggaaa tggttcctgc
     5641 ggaagtcatc agcaaactgg caaatgccgg gattgtggtt tctgccggtc actccaacgc
     5701 gacgttgaaa gaagcaaaag ccggtttccg cgcggggatt acctttgcca cccatctgta
     5761 caacgcgatg ccgtatatta ccggtcgtga accgggcctg gcgggcgcga tcctcgacga
     5821 agctgacatt tattgcggta ttattgctga tggcctgcat gttgattacg ccaacattcg
     5881 taacgctaaa cgtctgaaag gcgacaaact gtgtctggtt accgacgcca ccgcgccagc
     5941 aggtgccaac attgaacagt tcatttttgc gggtaaaaca atatactacc gtaacggact
     6001 ttgtgtggat gagaacggta cgttaagcgg ttcatcctta accatgattg aaggcgtgcg
     6061 taatctggtc gaacattgcg gtatcgcact ggatgaagtg ctgcgtatgg cgacgctcta
     6121 tccggcgcgt gcgattggcg ttgagaaacg tctcggcaca ctcgccgcag gtaaagtagc
     6181 caacctgacc gcattcacac ctgattttaa aatcaccaag accatcgtta acggtaacga
     6241 ggtcgtaact caataagaga aagtatgaca ccaggcggac aagctcagat aggtaatgtt
     6301 gatctcgtaa aacagcttaa cagcgcggcg gtttatcgcc tgattgacca gtacgggcca
     6361 atctcgcgga ttcagattgc cgagcaaagc cagcttgccc ccgccagcgt aaccaaaatt
     6421 acgcgtcagc ttatcgaacg cgggctgatc aaagaagttg atcagcaggc ctccaccggg
     6481 ggccgccgcg ctatctccat cgtcaccgaa acccgcaatt tccacgcaat cggcgtacgg
     6541 cttggtcgtc acgacgccac catcaccctg tttgatctca gcagcaaagt gctggcagaa
     6601 gaacattacc cgctgccgga acgtacccag caaacgctgg aacatgccct gttgaatgcc
     6661 attgctcagt ttattgatag ctaccagcgc aaactgcgcg agctgatcgc gatttcggtg
     6721 atcctgccag ggcttgttga cccggacagc ggcaaaattc attacatgcc gcatattcag
     6781 gtagaaaact gggggctggt agaagcactg gaagaacgtt ttaaagtgac ctgtttcgtt
     6841 ggtcacgata tccgtagtct ggcgctggcg gagcactact tcggtgcaag tcaggattgc
     6901 gaagactcca ttctggtgcg tgtccatcgc ggaaccgggg ccgggattat ctctaacggg
     6961 cgcattttta ttggccgcaa cggcaacgtc ggtgaaattg gccatattca ggtcgaaccg
     7021 ctgggtgaac gctgccactg cggcaacttt ggctgcctgg aaactatcgc tgccaacgcc
     7081 gctattgaac aacgggtgtt gaatctgtta aagcagggct accagagccg cgtgccgctg
     7141 gacgactgca ccatcaaaac tatctgcaaa gccgcgaaca aaggcgatag cctggcctcg
     7201 gaagtgattg agtatgtcgg tcgtcatctg ggcaaaacca tcgccattgc tatcaactta
     7261 tttaatccgc aaaaaattgt tattgccggt gaaatcaccg aagccgataa agtgctgctc
     7321 cctgctattg aaagctgcat taatacccag gcgctgaagg cgtttcgcac taatctgccg
     7381 gtggtacgtt ctgagctgga tcaccgctcg gcaatcggcg cttttgcgct ggtaaaacgc
     7441 gccatgctca acggtatttt gctccagcat ttgctggaaa attaatgtgc ttttatagtg
     7501 gcgcttattg ttgtcaatat tctgggtagt ccatgaccat taaaaatgta atttgcgata
     7561 tcgacggcgt gctgatgcac gataacgtcg ccgtaccggg tgcagcggaa tttttgcacg
     7621 ggattatgga taaaggcctg ccgctggtgt tgctgaccaa ctatccttcg cagaccgggc
     7681 aagatctggc gaaccgcttt gccactgccg gtgtcgatgt gccggatagc gtgttttata
     7741 cctctgcgat ggcgactgcc gatttcctgc gtcgccagga aggcaagaaa gcgtatgtgg
     7801 tgggcgaagg cgcactgatt catgaactgt acaaagccgg tttcactatt accgatgtga
     7861 accctgattt tgtgattgtt ggcgaaacgc gttcctacaa ctgggacatg atgcataaag
     7921 cggcctattt cgtcgctaac ggtgcacgct ttatcgccac caacccggac acccacgggc
     7981 gcggttttta tcccgcttgt ggcgcgttgt gtgcagggat tgagaaaatc tccgggcgca
     8041 aaccgttcta tgttggtaag cccagcccgt ggatcatccg cgcggcatta aacaaaatgc
     8101 aggcgcattc ggaagaaacg gtgattgtcg gcgataactt gcgcaccgac attctggcgg
     8161 gcttccaggc gggtctggag acgattctgg tgctttctgg tgtttcatcg ctcgacgata
     8221 tcgacagtat gccgttccgc ccaagctgga tttacccgtc ggtcgctgaa atcgacgtta
     8281 tctgaatagc aaccacgtct cagggcgtgg tttcttattt ttacccatca ttaaaatcat
     8341 cccattcaat aaaactcgcc aaaaattatc gatccatcaa taaatacatc accacaccct
     8401 cccctttttc acaaatcagc aatcaccatt acgtttttta tttttgcact gccaaatcgc
     8461 ttgcgcacgg tgctgctttg cggcattttt ttaaacaagc aaacacaaca agcaacaaat
     8521 accaggttaa cggagaaggt tatgtgttca atttttggcg tattcgatat caaaacagac
     8581 gcagttgagc tgcgtaagaa agcactcgag ctgtcacgcc tgatgcgtca tcgtggcccg
     8641 gactggtccg gtatttatgc cagcgataac gccattctcg cccacgaacg tctgtcaatt
     8701 gttgacgtta acgcgggggc gcaacctctc tacaaccaac aaaaaactca tgtgctggcg
     8761 gtaaacggtg aaatctacaa ccaccaggca ctgcgcgccg aatatggcga tcgttatcag
     8821 ttccagaccg gatctgactg cgaagtgatc ctcgcgctgt atcaggaaaa agggccggaa
     8881 tttctcgacg acttgcaggg catgtttgcc tttgccctgt acgacagcga aaaagatgca
     8941 tatctgattg gtcgcgacca tctggggatc atcccactgt atatgggcta tgacgagcac
     9001 ggtcagctgt atgtggcctc agaaatgaaa gccctggtgc cagtttgccg cacgattaaa
     9061 gagttcccgg cggggagcta tttgtggagt caggacggcg aaatccgttc ttattatcat
     9121 cgcgactggt tcgactttga tgcagtgaaa gataacgtga ccgacaaaaa cgagctgcgt
     9181 caggcactgg aagattccgt taaaagccat ctgatgtctg acgttcctta cggtgtgctg
     9241 ctctcgggtg gtctggattc ctcaattatt tccgcgatca ccaagaaata cgccgcccgt
     9301 cgcgtggaag atcaggaacg ctctgaagcc tggtggccgc aattacactc ctttgctgta
     9361 ggtctgccgg gttcaccgga tctgaaagca gcccaggaag tggcaaacca tctgggcacg
     9421 gtgcatcacg aaattcactt cactgtacag gaaggtctgg atgccatccg cgacgtgatt
     9481 taccacatcg aaacctatga tgtgacaact attcgcgctt caacaccgat gtatttaatg
     9541 tcgcgtaaga tcaaggcaat gggcattaaa atggtgctgt ccggtgaagg ttctgatgaa
     9601 gtgtttggcg gttatcttta cttccacaaa gcaccgaatg ccaaagaact gcatgaagag
     9661 acggtgcgta aactgctggc cctgcatatg tatgactgcg cgcgcgccaa caaagcgatg
     9721 tcagcctggg gcgtggaagc acgcgttccg ttcctcgaca aaaaattcct tgatgtggcg
     9781 atgcgcatta acccgcagga taaaatgtgc ggtaacggca aaatggaaaa acacatcctg
     9841 cgtgaatgtt ttgagtcata cctgcccgca agcgtggcct ggcggcagaa agagcagttc
     9901 tccgatggcg tcggttacag ttggatcgac accctgaaag aagtggcggc gcagcaggtt
     9961 tctgatcagc aactggaaac tgcccgcttc cgcttcccgt acaacacgcc aacctcaaaa
    10021 gaagcgtatc tgtaccggga gatctttgaa gaactgttcc cgcttccgag cgccgctgag
    10081 tgcgtgcctg gcggtccttc cgtcgcgtgt tcttccgcta aagcgattga gtgggatgag
    10141 gcgttcaaga aaatggacga tccgtctggt cgtgcggttg gcgttcacca gtcggcgtat
    10201 aagtaagaaa tgcgaaacag ccccggtgaa atccggggct gtttcagtta ttgaggtgct
    10261 tgttaattct gatatgcagt aaggaaatgt tccgggtttg ccacatcact gtttgacaac
    10321 tcatcactgt aggccagtat ttcatagatt aatttcccac cactattgtt gaattccgaa
    10381 tttcatcctg tacgacaaaa gaatggctgt agggtgggga aaaaacacaa agtaaacaat
    10441 aattgacgaa tatagcgcca cgctgttcgc aacctaacca aacagtcact ttcgagcaat
    10501 tttccttgaa aaagaggttg acgctgcaag gctctatacg cataatgcgc cccgcaacgc
    10561 cgataaggta tcgcgaaaaa aaagatggct acgtagctca gttggttaga gcacatcact
    10621 cataatgatg gggtcacagg ttcgaatccc gtcgtagcca ccatcttttt tgcgggagtg
    10681 gcgaaattgg tagacgcacc agatttaggt tctggcgccg caaggtgtgc gagttcaagt
    10741 ctcgcctccc gcaccattca ccagaaagcg ttgtacggat ggggtatcgc caagcggtaa
    10801 ggcaccggtt tttgataccg gcattccctg gttcgaatcc aggtacccca gccatcttct
    10861 tcgagtaagc ggttcaccgc ccggttattg gggtatcgcc aagcggtaag gcaccggttt
    10921 ttgataccgg cattccctgg ttcgaatcca ggtaccccag ccatcgaaga aacaatctgg
    10981 ctacgtagct cagttggtta gagcacatca ctcataatga tggggtcaca ggttcgaatc
    11041 ccgtcgtagc caccaaattc tgaatgtgtc gaatacgttc ggcaaattca aaaaccaatt
    11101 tgttggggta tcgccaagcg gtaaggcacc ggattctgat tccggcattc cgaggttcga
    11161 atcctcgtac cccagccaat ttattcaaga cgcttacatc gtaagtgcgc ccagttgggg
    11221 tatcgccaag cggtaaggca ccggattctg attccggcat tccgaggttc gaatcctcgt
    11281 accccagcca cattaaaaaa gctcgcttcg gcgagctttt tgcttttctg cgttcattca
    11341 atgtcgaatg cgatgttgac acgtcttatc cttcaatgtc ggatgcgacg ctgccgcgtc
    11401 ttatccgacc tacggttggc acgcatccgg caatgttgta aggctacaac cctaacgcat
    11461 atttcagcgc ctgacgtttc aacacgccag cacgctctgc cgccattaac ccgagattac
    11521 gcacaaaacg cagcggcggc agattattac tgaatccggc ataaaacaga tccataccac
    11581 tttgcataat gaagttatcc gccatgcgcc gcatctggta acgcttgagg acaggataac
    11641 tggcccacgc ttcaccgtag ctgcgggcat tcaccagaac atcaatcagg gcatcgacat
    11701 cacgataacc aagattcccc ccctgccccg ccagcggatg gatggtatgc gcggcatcgc
    11761 ccaccagcgc aagccccggc tgcacatact gcaacgcatg tcggcgcgtc agcggaaacg
    11821 caccagcggc aagcggtgta acgtaaccca gacgcgacgg gaaatgcttc gcgatttccg
    11881 cctggagttg cgccatattc atattctgca actggcgaat acgtaccgga gagtcatacc
    11941 acaccagcga tgcccagtta tcaaacaacg gcagaaacgc acgcggtccg tccggggtaa
    12001 attgctgcca ggtgctgtcg ccgggatcgt tctcacactg tacgctaatc aacatacacg
    12061 actgcgcata ctgccaggca tgaacgccaa ttcccgccat ctgccgcacc tgcgaatttg
    12121 cgccgtcagc accaatcacc agcttcgcgc gaatcacttc accgcccttc agttccagct
    12181 cctgaagatc attatctcga tgtaatgcaa tcagcgagcc tggcacacgt aacgttactt
    12241 tcggatgcgc ttccagcgcc tgccacaatg cctgttgcag gacggtgttt tccaccatat
    12301 agccaagcag cggcagctta agttcagcgg cgtcaaacat cacatgcgcc gtttcccact
    12361 cccacgtttc cagtctgcgg taaggatggc aacgcatagc ctgtactgca tcccagaccc
    12421 ctaacccttt aagcaatgat accgatgcgg cgctgatcgc cgagatccgc acgtccggtt
    12481 ggctatcagc gacaaacggt gcaggttctg cgtgctcgat caccgttacc gaaaatccgt
    12541 gctgtgccag ccccagcgcc agtgcgccgc cgaccattcc tccgccgaca atggcaattt
    12601 ccgttggttg atttgtcatg gtcaatcatc ctgttagcaa tatgctttaa gtttacagga
    12661 tttttacccc caagggctga taacgctcat actggtcaca acggcagcaa agcattacac
    12721 tatgcgccct gcattcctgg ctactatttc gcaagagcaa gtcgatgacc aaaaaactcc
    12781 atattaaaac ctggggctgt cagatgaacg agtacgattc atcgaagatg gccgatctgc
    12841 tggatgccac ccacggctat caactgaccg acgtggcgga agaagcggat gtgctgctgc
    12901 tgaacacctg ctcaatccgc gagaaggctc aggaaaaagt cttccatcag ttgggtcgct
    12961 ggaaactgtt aaaagagaag aatccagacc tgattatcgg cgtcggtggc tgcgtggcat
    13021 cgcaagaagg cgagcacatt cgccagcgcg cccactatgt cgatattatt tttgggccgc
    13081 aaacgctgca ccgtctgccg gagatgatca actccgtgcg cggcgaccgc agcccggttg
    13141 tagatatcag cttcccggaa atcgagaagt ttgaccgtct gccggaaccg cgcgccgaag
    13201 ggccgaccgc gtttgtctcc atcatggaag gctgcaataa atattgcacc tactgcgtgg
    13261 tgccttacac ccgtggtgaa gaggtaagcc gtccgtccga cgatattctg tttgagatcg
    13321 cccagcttgc ggctcagggc gtgcgtgaag tcaacctgct cggtcagaat gtgaatgcct
    13381 ggcgtggtga gaactacgac ggcaccaccg gatcgtttgc cgatctgttg cgtctggttg
    13441 ctgcgatcga cgggatcgat cgtattcgct ttaccaccag ccatccgatc gaattcaccg
    13501 acgatatcat cgaagtgtat cgcgacacgc cggagctggt gagcttcctg catctgccgg
    13561 tacagagcgg ttccgatcgc attctgaacc tgatgggccg tactcatacg gcactggagt
    13621 acaaagcgat catccgtaaa ctgcgtgcgg cgcgtccgga tattcagatc agttctgact
    13681 tcattgttgg cttccctggc gaaaccaccg aagacttcga gaaaacgatg aagctgattg
    13741 ccgacgtcaa tttcgacatg agctacagct ttatcttctc tgcacgtccg ggtacaccag
    13801 ccgccgatat ggttgatgat gttccggaag aagagaagaa gcaacgtctg tatattctgc
    13861 aagaacgtat caatcagcaa gcgatggcat ggagccgccg gatgctcggc accacccagc
    13921 gtattctggt agaagggact tcgcgtaaga gcatcatgga gctttccggg cgtaccgaaa
    13981 ataaccgcgt ggtcaacttc gagggcacac cggatatgat cggtaaattc gtcgatgtag
    14041 aaattaccga cgtctacccg aactctctgc gcggtaaagt ggtgcgtact gaagatgaaa
    14101 tgggtctgcg cgtggcagaa acaccggaat cggtgattgc ccgtacccgc aaagaaaacg
    14161 accttggcgt gggttattat cagccgtaat tctcaggccc gccgttccgg tgggccttgt
    14221 tttttctctc tttatcccca tctttatcgc aatgcttgcg cctttttcct gtggcgtgaa
    14281 taatttcctt ataggccggt gaatgttcag cttccggcga catacagata agaggaacgg
    14341 tttgaacata gacactcgcg aaatcaccct ggagccagca gacaacgcgc gtctgttgag
    14401 cctgtgcggc ccgtttgatg acaacatcaa gcagctcgaa cgccgtctcg gcatcgagat
    14461 caatcgccgc gacaaccact ttaaactgac cggccgtccg atttgcgtca ccgctgcggc
    14521 agacattctg cgtagcctgt atgtcgatac agccccgatg cgcggtcaga ttcaggatat
    14581 cgaaccggaa cagatccacc ttgcgattaa agaagcgcga gtactggagc aaagtgcgga
    14641 gagcgtgccg gagtacggca aagcggtcaa tatcaaaacc aaacgcggcg taattaagcc
    14701 gcgcacgcca aaccaggcgc agtacatcgc caatattctc gaccatgaca ttaccttcgg
    14761 cgttggcccg gcgggtacgg gtaaaaccta cctggcagtg gctgcggcag ttgatgccct
    14821 ggagcgtcag gagattcgcc gtattctgct gactcgtccg gcggtcgaag ccggtgagaa
    14881 actgggcttc ctgcctggcg atttaagcca gaaagtagac ccgtatctgc gcccgctgta
    14941 cgacgcgctg tttgaaatgc tgggctttga gaaagttgag aaactgattg agcgcaacgt
    15001 tattgaagtc gcgccgctgg cctatatgcg tggtcgtacg ctgaacgacg cgtttatcat
    15061 tctcgatgag agccagaaca ccaccatcga acagatgaag atgttcctga cccgtatcgg
    15121 ttttaactca aaagcggtta tcaccggcga cgtcacacag atcgacctgc cgcgtaatac
    15181 taaatcaggc ttacgtcacg ccatcgaagt gctggccgat gtcgaagaga tcagctttaa
    15241 cttcttccac agcgaagacg tggttcgtca cccggtggtg gcgcgtatcg ttaacgccta
    15301 tgaagcctgg gaagaagccg aacaaaaacg aaaagcggcg ctggcggcag aacgcaagcg
    15361 cgaagaacag gaacaaaaat gagtcaggtg atcctcgatt tacaactggc atgtgaagat
    15421 aattccgggt taccggaaga gagccagttt caaacatggc tgaatgcggt gatcccgcag
    15481 tttcaggaag aatcggaagt gactattcgc gtggtcgata ccgccgaaag ccacagtctg
    15541 aatctgacct atcgcggtaa ggataagccg accaacgtgc tctccttccc gtttgaagtg
    15601 ccgcctggca tggaaatgtc gctactgggc gatctggtta tctgccgtca ggtggttgag
    15661 aaggaagctc aggagcaagg caaaccactg gaggcgcact gggcgcatat ggtggtgcac
    15721 ggcagtctgc atttgttagg ttacgatcac atcgaagatg acgaagcaga agaaatggaa
    15781 gccctcgaaa cagagattat gcttgctctg ggctatgagg atccgtacat tgccgagaaa
    15841 gaataatttg ccaggcattt gactggcaga cgtccccgcc gttggtgatt aacgacggcg
    15901 ttttgattaa ctaacatgag aacccatacg acgccatgag cgacgacaat tcacacagta
    15961 gtgacacgat aagcaacaag aagggatttt tctccctgtt actcagccaa cttttccacg
    16021 gtgaaccgaa aaaccgtgac gaactgctgg cgctgatccg tgattccggg cagaacgacc
    16081 ttatcgacga agatacgcgc gatatgctcg aaggggtgat ggacatcgca gaccaacgcg
    16141 ttcgcgacat catgatcccc cgctcccaga tgattaccct gaaacgcaac cagacgctgg
    16201 acgaatgcct tgatgtcatc atcgagtccg cccactcacg tttcccggtg attagcgaag
    16261 acaaagatca cattgaaggg attctgatgg cgaaagactt gctaccgttt atgcgcagcg
    16321 atgctgaagc cttcagcatg gacaaagtgt tacgtcaggc ggttgtcgtt cctgaaagta
    16381 agcgcgtaga ccggatgctg aaagagtttc gctctcagcg ttaccacatg gcgatcgtta
    16441 ttgacgaatt cggtggggtt tccggtctgg tgaccattga agacatcctg gaactgattg
    16501 ttggtgagat tgaagacgag tatgacgaag aagatgatat cgacttccgt cagctgagtc
    16561 gtcatacctg gaccgtgcgc gcactggctt ccattgaaga cttcaacgaa gcgttcggca
    16621 cccactttag cgatgaagaa gtcgacacta tcggtggtct ggtgatgcag gcgtttgggc
    16681 atcttccggc gcgtggcgaa actatcgaca tcgacggtta ccagttcaaa gtggcgatgg
    16741 ccgatagtcg gcgtattatt caggttcatg tcaaaatccc ggatgactca ccccagccaa
    16801 agctggatga ataaaaccga aactggatag ataactacat ggcttttgcc tcattaattg
    16861 aacgccagcg cattcgcctg ctgctggcgt tattattcgg tgcctgcgga acgctggcct
    16921 tctctcctta cgatgtctgg cctgcggcga ttgtttcgct gatggggctt caggcgctga
    16981 cctttaaccg ccgcccgctc cagtctgccg ctattggctt ctgctgggga tttggcctct
    17041 ttggcaccgg tattaactgg gtctatgtca gcatcgcgac ctttggcggg atgcctggcc
    17101 cggttaacat cttcctggtg gtgctgctgg cggcgtattt gtcgctgtat accggactgt
    17161 ttgctggcgt gctgtcgcgc ctgtggccga aaaccacctg gctgcgcgtg gcgattgccg
    17221 cccctgctct ctggcaagtg accgagtttc tgcgcggttg ggtgctgact ggcttcccgt
    17281 ggttacagtt cggctatagc cagattgatg gcccgttaaa agggctggca ccaataatgg
    17341 gcgtggaagc cattaacttc ctgctgatga tggttagcgg cctgctggca ctggcgttag
    17401 tcaaacgcaa ctggcgtccg ctggtggtgg ccgtcgtgct gtttgccctt cccttcccgc
    17461 tgcgttacat ccaatggttt accccgcaac cggagaaaac cattcaggtt tcgatggttc
    17521 agggcgatat tccgcaatca ctgaaatggg acggagacca gcttcttaat acgctgaaga
    17581 tttactacaa cgcaacggca ccgctgctgg gcaaatcatc gttgattatc tggccggagt
    17641 cggcgataac cgatctggaa attaatcagc aaccgttcct caaagcactg gacggtgagt
    17701 tgcgtgataa aggtagctcg ctggtaaccg ggattgtcga cgcgcgtctc aataagcaga
    17761 accgctacga tacctacaac accatcatca cgctgggtaa aggtgcaccg tacagctacg
    17821 aatcagccga tcgctataac aaaaaccatc tggtgccgtt tggcgagttt gtcccgctgg
    17881 agtcgattct gcgtccgtta gcaccgttct ttgatctgcc gatgtcgtcg ttcagccgtg
    17941 ggccatatat ccagccgccg ctgtcggtaa atggtattga gcttactgcg gccatttgct
    18001 acgagatcat tcttggcgag caagtgcgcg ataacttccg cccggatacc gactatctgc
    18061 tgactatctc caacgatgcg tggtttggta agtctattgg tccatggcag cacttccaga
    18121 tggcgcgaat gcgtgcgctg gagctggcgc gcccactgtt gcgcagcacc aacaacggca
    18181 ttacggcggt gattggcccg cagggtgaga ttcaggcgat gatcccgcag ttcacccgcg
    18241 aggtgttgac cactaatgtg acaccgacca ccggactcac accgtatgca cgtaccggca
    18301 actggccgct gtgggtgctg acggcgctgt ttggttttgc cgctgtgctg atgagtctgc
    18361 gagcacgtaa acgttgatgt gcctgatgta tcctgcatga gcaaaacgta ctttctcatt
    18421 gagctttggg gcatggttat catgcccctt tttcttacgg ttcagggata caagccacat
    18481 tgccatattg gggatgacga ataattgcct gcggatcaag cgagcgcgtc ctgtccattt
    18541 ggatcaaccc gttcttcgcc atgacaaata tttttctacc atccgctgaa aaacgaaggt
    18601 gattaacgta gctggcagga taaacgccaa taatctgccc ggcattaata ttgagcagga
    18661 tgacataacc ttgcaggcca tatgacacca acgctaaata ttgaccgtcc gggctaatac
    18721 cctcaacgga ataaacgctc ctgctacacc attgcgtcat aatgctggct tgttgatccc
    18781 ctacgcttat acgtgaaatc ccctgactat cggcaaaaat aacctcgccg ttttcaggtc
    18841 gaaaacgcat cgccgatatc gttccacgcg catcacctgc gggaaagtct tctttctgat
    18901 gcgtgatggt attgatccgc acgatgttac ctctgcctgc tcccggaacc agtaatgttc
    18961 tttcatccgg ggaaagtgcc ataacccagg cgcctggttt accgctcgtc cccgtcccgt
    19021 cgtatttctc cagcgggata tccgccacat gctgataagt caccagatca atcgctgaca
    19081 cactattact ggtgcgattc atcacccagg cttttgtgcc atcacgcgag aggataaaac
    19141 tgtcaggatg tgcaccggtg gtaattgact gaataatctc ccctgtcgcc acgttaatga
    19201 ccgataacgc attcgcttca aatgacgtcg cataaatcaa cttttcatcc gggcttaatg
    19261 ccagcgatct gacgccctga tctttacagc caccacaatg tttgactgac ttttctttcg
    19321 ctatgggcgc tgtaaaggta tgcaggattt tcttttgtcc gaggtcgaaa acaacaatgc
    19381 tgttggtgtt ttcaattgcg gcatacaacg tattaccatc ccgactgatc gccatatcat
    19441 ggatatcggc aatctcagcg agtaaatcac cactggcctg ttcacgactt accggagcgg
    19501 ataatgtttc cctgagggcg ttgcgttcct ccgctaactg ttgttttttt tgtcgtcgct
    19561 cttgttcaat ctcctgcgga taaaacgctt cacgcaggct ttgcctgacc tgtaaaactg
    19621 actcatccat ctgctgctgc gcaagccaga tagccccggt cagaagaaga ctgcccgcaa
    19681 caataatccc gccttccgta acgagtctgt tttgcccaga taaacgcgcg ctcagccaac
    19741 ccaacgcgcc acccgcaatc aggccgccaa tgtggcaagc gttatctatt cctgtttgcc
    19801 gggcaccgta caacagtgtc agcgccacca tacccaacag aggaaatact cgccgctggt
    19861 ttttatggta tgtgcccgta cctgaagcaa actgtgttgc tatcgctgca ccagctattc
    19921 ccagaatcgc cccggatgcc ccaacgctga tgactatctg ctcactttcg cgtaacgcat
    19981 aacaggcgct gatgagccca ccaaagacgc cggagaataa ccaggtaatc aggagtcgcc
    20041 atttccccag aatctgttcc gccacgcctc ccacgaccag gaatgcaaac atattcatca
    20101 gcagatgaga aacactactg tgcagaaaga tactgctcaa taagcgccag ggttcacccg
    20161 acaaagtaag aggcgcaacg tttgcaccga gatggataag tgcggattga tcgacctctt
    20221 cataactcat ccattgtgcg agataacaaa atatcccaat acagagtgca aaaaggataa
    20281 gggttattgc gggtaattga acatttaacg gcttaaccga agatgcagac ataaggtgtt
    20341 tcttccttga aaagacaggt aaaaagcatt tttcccgtgt gcagttatca tcccggcaaa
    20401 cgtcccatgc cgaaaagata attctgtccg aaatatgact ttttcagcct aaaattacaa
    20461 tcgtaattaa ttcgccccta agtatttttt ggcatgtaag ttgctttatt tactgtgtaa
    20521 gcgatgaaat cagccgcgac gcaaccaggt tgcaccacca caaatcagcg gtagcaccaa
    20581 aaaggtgcgg tttaagaatt ttgcttcgga atggtgcggc gcatctcgca aaaacaaaca
    20641 ataaaacagc aatatcttta catttagcca aactaaatgt tacctaacag gcacaacact
    20701 gcacaataaa gttgcagacg ataacaacac aaacactcac aacgggtatc catgcgttct
    20761 taacgcagaa gataaaggag ttggatatgc aattacgtaa acctgccaca gcaatcctcg
    20821 ccctggcgct ttccgcagga ctggcacagg cagatgacgc cgccccggta gcgggcagta
    20881 cgctggacaa aatcgccaaa aacggcgtga ttgtcgtcgg tcaccgtgaa tcttcagtac
    20941 ctttctctta ttacgacaat cagcaaaaag tggtgggtta ctcgcaggat tactccaacg
    21001 ccattgttga agcagtgaaa aagaaactca acaaaccgga cttgcaggta aaactgattc
    21061 cgattacctc acaaaaccgt attccactgc tgcaaaacgg cactttcgat tttgaatgtg
    21121 gttctaccac caacaacgtc gaacgccaaa aacaggcggc tttctctgat actattttcg
    21181 tggtcggtac gcgcctgctg accaaaaagg gcggcgatat caaagatttt gccgacctga
    21241 aaggcaaagc cgtagtcgtc acttccggca ctacttctga agttttgctc aacaaactga
    21301 atgaagagca aaaaatgaat atgcgcatca ttagcgccaa agatcacggt gactctttcc
    21361 gtaccctgga aagcggtcgt gccgttgcct ttatgatgga tgatgccctg ctggccggtg
    21421 aacgtgcgaa agcgaagaaa ccagacaact gggatatcgt cggcaagccg cagtctcagg
    21481 aagcctacgg ttgcatgttg cgtaaagatg atccgcagtt caaaaagctg atggatgaca
    21541 ctatcgctca ggtgcagact tccggtgaag cggaaaaatg gtttgataaa tggttcaaaa
    21601 atccaattcc gccgaaaaac ctgaacatga atttcgaact gtcagacgaa atgaaagcac
    21661 tgttcaaaga accgaatgac aaagcactga actaattaca agaaccaggg gcggaaattc
    21721 cagccctctc gattgttacg tagcacggac agactatacg cctgatggtc gttccccatc
    21781 gggcctgaaa accgcaatac gctgggtaac aatcttcgag ggtagcagtt aacgctgcta
    21841 ccctcttttt tctggagtag atttatgtct atagactgga actggggtat ttttttacaa
    21901 caagccccgt tcggcaacac cacctatctt ggttggatct ggagtggttt tcaggtgacg
    21961 atcgctttgt cgatctgcgc ctggattatc gctttcctcg tcggttcatt tttcggcatt
    22021 ttacgtaccg ttccaaaccg ttttctctcc ggtctgggta cgttgtatgt cgaactgttc
    22081 cgcaacgtgc cgttgattgt gcaattcttt acctggtatc tggtgatccc ggagttgctg
    22141 ccagagaaaa tcggtatgtg gtttaaggcc gagctggatc ccaatattca gtttttcctt
    22201 tcatccatgc tctgcctggg gctgtttacc gccgcccgtg tttgcgaaca ggtacgcgcc
    22261 gccattcagt cgttgccgcg tgggcaaaaa aatgccgcgc tggcgatggg cctgacgcta
    22321 ccgcaggctt atcgatacgt actgctgcct aatgcttatc gcgttatcgt cccgccgatg
    22381 acttccgaga tgatgaacct ggtgaaaaac tccgccatcg cctcgactat tggtctggtg
    22441 gatatggcgg cgcaggcggg taaattgctt gattactcag cccacgcctg ggagtcgttt
    22501 accgctatta cgctggcgta tgtgttgatt aacgcggtta tcatgctggt aatgacgctg
    22561 gttgaacgta aagttcgcct gcctggcaac atggggggca aataatgtac gagtttgact
    22621 ggagttccat tgtcccttcc ctgccatatc tgctcgacgg gctggtgatc acgctgaaaa
    22681 tcaccgtcac ggcagtcgtg attggtattt tgtggggcac catgctggcg gtgatgcgct
    22741 tgtccagctt tgcgcccgta gcgtggtttg ccaaagccta cgttaacgta ttccgctcga
    22801 ttcctttagt tatggttttg ctgtggtttt acctgatcgt gccaggtttt ctgcaaaacg
    22861 tgctgggatt atcaccaaaa aatgatatcc gtttaatttc ggcgatggtg gcgttttcaa
    22921 tgtttgaagc ggcctactat tcagagatta tccgtgctgg tattcaaagt atttctcgtg
    22981 gtcagtcgag cgccgcgctg gcgttgggaa tgactcactg gcagtcgatg aaactgatta
    23041 ttctgccgca ggcgttccgc gcgatggtgc cgcttctgct cactcagggc atcgtactgt
    23101 tccaggatac ctcactggtg tatgtgttat ctctggccga tttcttccgt accgcctcaa
    23161 ccattggtga gcgtgatggt acgcaggttg agatgatcct atttgccgga tttgtttatt
    23221 tcgttattag tcttagcgcg tcgttgttgg tcagctactt gaaaagaagg acagcataat
    23281 gattaccctg aaaaatgttt caaaatggta tggtcacttt caggtgctga ccgactgctc
    23341 aaccgaagtg aaaaaaggcg aagtggtggt ggtttgcggc ccgtctggtt ccggcaaatc
    23401 aacgctgatt aaaaccgtca acggcctcga accagtgcag caaggtgaaa tcaccgtcga
    23461 tggtatcgtg gttaatgaca agaaaaccga tctggcaaag ctgcgttccc gcgtcgggat
    23521 ggtgttccag cattttgagt tgttccccca tttgtcgatt attgaaaacc tgactctggc
    23581 gcaggtgaaa gtgcttaaac gcgataaagc gcccgctcgt gaaaaagcat tgaaattgct
    23641 ggagcgcgtg ggactttccg cacatgccaa taaatttccg gcgcagcttt ccggcggtca
    23701 gcagcagcgt gtggcaatcg ctcgcgcgtt gtgtatggat cctattgcga tgctgttcga
    23761 cgaaccgaca tcggcgctgg atccggagat gatcaacgaa gtgctggacg tgatggtgga
    23821 actggcgaac gaaggaatga ccatgatggt ggtgacccac gaaatgggct ttgcccgcaa
    23881 agtggcgaat cgggtgatct ttatggacga gggtaaaatt gtcgaagact cgccgaaaga
    23941 cgctttcttc gatgatccga aatcggaccg cgcgaaagac ttcctcgcga aaatcctgca
    24001 ttaatcactc tggcgcgcat acccaagtgc gcgccatctc acgctttatc gtttcgcccc
    24061 tcgtatctgt tacatcctgg cgttaccctt gtcgcaaaga agcacacaac aaggagcaac
    24121 aatggcactg ccaattctgt tagattgcga cccaggtcat gacgacgcta tcgcaatagt
    24181 tctcgccctc gcctcaccag agcttgatgt caaagcaatt acgtcttccg ccggaaacca
    24241 gacaccagaa aaaaccttac gcaatgttct gcgtatgctg accttgctta atcgcaccga
    24301 tattccggta gcaggcggcg cggtaaaacc gttaatgcgt gagttgatta tcgcggacaa
    24361 tgtgcacggc gaaagcggtc tggatggccc ggcattaccg gaaccgacgt tcgcaccgca
    24421 aaactgtacg gcggtagagc tgatggcgaa aacgctgcgc gaaagtgcgg aacctgtcac
    24481 gattgtgtct accggaccgc aaactaacgt tgccttgctg ctcaatagcc atccggaact
    24541 gcatagcaaa attgcccgta tcgtgattat gggcggcgca atggggcttg gtaactggac
    24601 gcctgcggct gaatttaata tttacgttga cccggaagcg gcagaaattg tcttccagtc
    24661 agggatccca gtggtgatgg ccggtctgga tgttactcac aaagcacaaa tccacgttga
    24721 agacaccgag cgtttccgcg cgattggcaa ccctgtttca accattgttg ccgaactgct
    24781 ggatttcttc ctcgaatatc ataaagacga aaaatggggc tttgtcggtg caccgctgca
    24841 tgacccatgc accatcgcct ggctgttgaa acccgagttg tttaccactg ttgagcgctg
    24901 ggttggcgtg gaaacacagg ggaaatatag ccagggtatg acggttgttg attattatta
    24961 tctgactggc aataaaccga atgccactgt aatggtcgat gttgatcgtc agggctttgt
    25021 tgatttactg gctgatcgtc tgaaatttta cgcttaacac acgtcattga ttaagatcac
    25081 cacgcgttac ctgggtggtc ttaatctgtt tatctctttt tctttca
//
LOCUS       DAZKHF020000046        24601 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2201S31241
            SAMN43267800-rid25106413.denovo.046, whole genome shotgun sequence.
ACCESSION   DAZKHF020000046 DAZKHF020000000
VERSION     DAZKHF020000046.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267800
            Sequence Read Archive: SRR30307263
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 24601)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24601)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 24601)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350581.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350581.2
            Genome Coverage       :: 151x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:21:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,652
            CDSs (total)                      :: 5,557
            Genes (coding)                    :: 5,320
            CDSs (with protein)               :: 5,320
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 237
            CDSs (without protein)            :: 237
            Pseudo Genes (ambiguous residues) :: 0 of 237
            Pseudo Genes (frameshifted)       :: 102 of 237
            Pseudo Genes (incomplete)         :: 130 of 237
            Pseudo Genes (internal stop)      :: 52 of 237
            Pseudo Genes (multiple problems)  :: 41 of 237
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24601
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267800-rid25106413.denovo.046"
                     /serotype="O 26:H11"
                     /isolate="2201S31241"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-01"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            429..1568
                     /gene="wza"
                     /locus_tag="ACHOSC_RS07545"
                     /old_locus_tag="pgaptmp_001509"
     CDS             429..1568
                     /gene="wza"
                     /locus_tag="ACHOSC_RS07545"
                     /old_locus_tag="pgaptmp_001509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide export protein Wza"
                     /protein_id="HGU4547476.1"
                     /translation="MMKSKMKLMPLLVSVTLISGCTVLPGSNMSTMGKDVIKQQDADF
                     DLDKMVNVYPLTPRLIDQLRPRPNVARPNMTLESEIANYQYRVGPGDVLNVTVWDHPE
                     LTTPAGQYRSSSDTGNWVQPDGTMFYPYIGKVHVVGKTLAEIRSDITGRLATYIADPQ
                     VDVNIAAFRSQKAYISGQVNKSGQQAITNVPLTILDAINAAGGLTDTADWRNVVLTHN
                     GREERISLQALMQNGDLNQNRLLYPGDILYVPRNDDLKVFVMGEVKKQSTLKMDFSGM
                     TLTEALGNAEGIDMTTSNASGIFVIRPLKGEGGRNGKIANIYQLDMSDATSLVMATEF
                     RLQPYDVVYVTTAPVSRWNRLINQLLPTISGVRYMTDTASDIHNW"
     gene            1574..2017
                     /gene="wzb"
                     /locus_tag="ACHOSC_RS07550"
                     /old_locus_tag="pgaptmp_001510"
     CDS             1574..2017
                     /gene="wzb"
                     /locus_tag="ACHOSC_RS07550"
                     /old_locus_tag="pgaptmp_001510"
                     /EC_number="3.1.3.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="low molecular weight
                     protein-tyrosine-phosphatase Wzb"
                     /protein_id="HGU4547477.1"
                     /translation="MFNNILVVCVGNICRSPTAERLLQRYHPELKVESAGLGALVGKG
                     ADPTAISVAAEHQLSLEGHCARQISRRLCRNYDLILTMEKRHIERLCEMAPEMRGKVM
                     LFGHWDNECEIPDPYRKSRETFAAVYTLLERSARQWAQALNAEQV"
     gene            2020..4182
                     /gene="wzc"
                     /locus_tag="ACHOSC_RS07555"
                     /old_locus_tag="pgaptmp_001511"
     CDS             2020..4182
                     /gene="wzc"
                     /locus_tag="ACHOSC_RS07555"
                     /old_locus_tag="pgaptmp_001511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416564.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-protein kinase Wzc"
                     /protein_id="HGU4547478.1"
                     /translation="MTEKVKQHAAPVTGSDEIDIGRLVGTVIEARWWVIGITAVFALC
                     AVVYTFFATPIYSADALVQIEQNSGNSLVQDIGSALANKPPASDAEIQLIRSRLVLGK
                     TVDDLDLDIAVSKNTFPIFGAGWDRLMGRQNETVKVTTFNRPKEMADQVFTLNVLDNK
                     NYTLSSDGGFSARGQAGQMLKKEGVTLMVEAIHARPGSEFTVTKYSTLGMINQLQNSL
                     TVTENGKDAGVLSLTYTGEDREQIRDILNSIARNYQEQNIERKSAEASKSLAFLAQQL
                     PEVRSRLDVAENKLNAFRQDKDSVDLPLEAKAVLDSMVNIDAQLNELTFKEAEISKLY
                     TKVHPAYRTLLEKRQALEDEKAKLNGRVTAMPKTQQEIVRLTRDVESGQQVYMQLLNK
                     EQELKITEASTVGDVRIVDPAITQPGVLKPKKGLIILGAIILGLMLSIVGVLLRSLFN
                     RGIESPQVLEEHGISVYASIPLSEWQKARDSVKTIKGIKRYKQSQLLAVGNPTDLAIE
                     AIRSLRTSLHFAMMQAQNNVLMMTGVSPSIGKTFVCANLAAVISQTNKRVLLIDCDMR
                     KGYTHELLGTNNVNGLSEILIGQGDITTAAKPTSIAKFDLIPRGQVPPNPSELLMSER
                     FAELVNWASKNYDLVLIDTPPILAVTDAAIVGRHVGTTLMVARYAVNTLKEVETSLSR
                     FEQNGIPVKGVILNSIFRRASAYQDYGYYEYEYKSDAK"
     gene            4275..5114
                     /gene="wcaA"
                     /locus_tag="ACHOSC_RS07560"
                     /old_locus_tag="pgaptmp_001512"
     CDS             4275..5114
                     /gene="wcaA"
                     /locus_tag="ACHOSC_RS07560"
                     /old_locus_tag="pgaptmp_001512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis glycosyltransferase
                     WcaA"
                     /protein_id="HGU4547479.1"
                     /translation="MKDNPLISIYMPTWNRQQLAIRAIKSVLRQDYSNWEMIIVDDCS
                     TSWEQLQQYVTALNDPRITYIHNDINSGACAVRNQAIMLAQGEYITGIDDDDEWTPNR
                     LSVFLAHKQQLVTHAFLYANDYVCQGEVYSQPASLPLYPKSPYSRRLFYKRNIIGNQV
                     FTWAWRFKECLFDTELKAAQDYDIFLRMVVEYGEPWKVEEATQILHINHGEMQITSSP
                     KKFSGYFHFYRKHKDKFDRASKKYQLFTLYQIRNKRMTWRTLLTLLSVRNGKRLADGI
                     RGR"
     gene            5117..5605
                     /gene="wcaB"
                     /locus_tag="ACHOSC_RS07565"
                     /old_locus_tag="pgaptmp_001513"
     CDS             5117..5605
                     /gene="wcaB"
                     /locus_tag="ACHOSC_RS07565"
                     /old_locus_tag="pgaptmp_001513"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis acetyltransferase
                     WcaB"
                     /protein_id="HGU4547480.1"
                     /translation="MLEDLRANSWSLRPCCMVLAYRVAHFCSVWRKKNVLNNLWAAPL
                     LVLYRIITECFFGYEIQAAATIGRRFTIHHGYAVVINKNVVAGDDFTIRHGVTIGNRG
                     ADNMACPHIGNGVELGANVIILGDITLGNNVTVGAGSVVLDSVPDNALVVGEKARVKV
                     IK"
     gene            5602..6819
                     /gene="wcaC"
                     /locus_tag="ACHOSC_RS07570"
                     /old_locus_tag="pgaptmp_001514"
     CDS             5602..6819
                     /gene="wcaC"
                     /locus_tag="ACHOSC_RS07570"
                     /old_locus_tag="pgaptmp_001514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis glycosyltransferase
                     WcaC"
                     /protein_id="HGU4547481.1"
                     /translation="MNILQFNVRLAEGGAAGVALDLHQRALQQGLASHFVYGYGKGGK
                     ESVSHQNYPQVVKHTPRMTAMANIALFRLFNRDLFGNFNELYRTITCTPGPVVLHFHV
                     LHSYWLNLKSVVRFCEKVKNHKPDVTLVWTLHDHWSVTGRCAFTDGCEGWKTGCQKCP
                     TLNNYPPVKIDRAHQLVAGKRQLFREMLALGCQFISPSQHVADAFNSLYGPGRCRIIN
                     NGIDMATEAILADLPPVRETQGKPKIAVVAHDLRYDGKTNQQLVREMMALGDKIELHT
                     FGKFSPFTAGNVVNHGFETDKRKLMSALNQMDALVFSSRVDNYPLILCEALSIGVPVI
                     ATHSDAAREVLQKSGGKTVSEEDVLQLVQLSKPEIAQAIFGTTLAEFSQRSRAAYSGQ
                     QMLEEYVNFYQNL"
     gene            6794..8011
                     /gene="wcaD"
                     /locus_tag="ACHOSC_RS07575"
                     /old_locus_tag="pgaptmp_001515"
     CDS             6794..8011
                     /gene="wcaD"
                     /locus_tag="ACHOSC_RS07575"
                     /old_locus_tag="pgaptmp_001515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid polymerase WcaD"
                     /protein_id="HGU4547482.1"
                     /translation="MSTSIRICSYLLLPLIYLLVNVKIAQLGESFPITIVTFLPVLLL
                     LFLERISVKKLMIALGIGAGLTAFNYLFGQSLDASKYVTSTMLFVYIVIIIGMVWSIR
                     FKTISPHNHRKILRFFYLVVGLVVALAAVEMAQIILTGGSSIMESISKYLIYSNSYVL
                     NFIKFGGKRTTALYFEPAFFALALISIWLSIKQFGIKTPKTDAMILAGIILSGSFSGV
                     MTFILFYLLEWAFQYLNKEAIKKKLPLALISLAVFLVGVVIAFPYISTRLGDLGTEGS
                     SSYYRIVGPLVMVGYSLTHIDGVVRFGSLYEYVASFGIFNGADVGKTIDNGLYLLIIY
                     FSWFAVFLSLWYMGKVIKMMINAFGDNRNFRVQLYLFTPVSLFFTGSIFSPEYAFLIV
                     CPFILRKALNITR"
     gene            8022..8768
                     /gene="wcaE"
                     /locus_tag="ACHOSC_RS07580"
                     /old_locus_tag="pgaptmp_001516"
     CDS             8022..8768
                     /gene="wcaE"
                     /locus_tag="ACHOSC_RS07580"
                     /old_locus_tag="pgaptmp_001516"
                     /EC_number="2.4.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis glycosyltransferase
                     WcaE"
                     /protein_id="HGU4547483.1"
                     /translation="MLLSIITVAFRNLEGIVKTHASLAHLAQAEDISFEWIVVDGGSN
                     DGTREYLENLNGIYNLRFVSEPDNGIYDAMNKGITMAQGKFALFLNSGDIFHQDAANF
                     VRKLKMQKDNMMITGDALLDFGDGHKIKRSAKPGWYIYHSLPASHQAIFFPVSGLKKW
                     RYDLEYKVSSDYALAAKMYKAGYAFKKLNGLVSEFSMGGVSTTNNMELCADAKKVQRQ
                     ILHVPGFWAELSWHLRQRTTSKTKALYNKV"
     gene            8784..9332
                     /gene="wcaF"
                     /locus_tag="ACHOSC_RS07585"
                     /old_locus_tag="pgaptmp_001517"
     CDS             8784..9332
                     /gene="wcaF"
                     /locus_tag="ACHOSC_RS07585"
                     /old_locus_tag="pgaptmp_001517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis acetyltransferase
                     WcaF"
                     /protein_id="HGU4547484.1"
                     /translation="MQDLSGFSVPKGFRGGNAIKVQLWWAVQATIFAWSPQVLYRWRA
                     FLLRLFGAKIGKNVVIRPSVKITYPWKLTLGDYAWVGDDVNLYTLGEITIGAHSVISQ
                     KSYLCTGSHDHASQHFTINATPIVIGEKCWLATDVFVAPGVTIGNGTVVGARSSVFKS
                     LPANVVCRGNPAVVIRERVETE"
     gene            9358..10479
                     /gene="gmd"
                     /locus_tag="ACHOSC_RS07590"
                     /old_locus_tag="pgaptmp_001518"
     CDS             9358..10479
                     /gene="gmd"
                     /locus_tag="ACHOSC_RS07590"
                     /old_locus_tag="pgaptmp_001518"
                     /EC_number="4.2.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005128297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008446 - GDP-mannose 4,6-dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0000271 - polysaccharide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0019673 - GDP-mannose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-mannose 4,6-dehydratase"
                     /protein_id="HGU4547485.1"
                     /translation="MSKVALITGVTGQDGSYLAEFLLEKGYEVHGIKRRASSFNTERV
                     DHIYQDPHTCNPKFHLHYGDLSDTSNLTRILREVQPDEVYNLGAMSHVAVSFESPEYT
                     ADVDAMGTLRLLEAIRFLGLEKKTRFYQASTSELYGLVQEIPQKETTPFYPRSPYAVA
                     KLYAYWITVNYRESYGMYACNGILFNHESPRRGETFVTRKITRAIANIAQGLESCLYL
                     GNMDSLRDWGHAKDYVKMQWMMLQQEQPEDFVIATGVQYSVRQFVEMAAAQLGIKLRF
                     EGTGVEEKGIVVSVTGHDAPGVKPGDVIIAVDPRYFRPAEVETLLGDPTKAHEKLGWK
                     PEITLREMVSEMVANDLEAAKKHSLLKSHGYDVAIALES"
     gene            10482..11447
                     /gene="fcl"
                     /locus_tag="ACHOSC_RS07595"
                     /old_locus_tag="pgaptmp_001519"
                     /gene_synonym="wcaG"
                     /gene_synonym="yefB"
     CDS             10482..11447
                     /gene="fcl"
                     /locus_tag="ACHOSC_RS07595"
                     /old_locus_tag="pgaptmp_001519"
                     /gene_synonym="wcaG"
                     /gene_synonym="yefB"
                     /EC_number="1.1.1.271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-L-fucose synthase"
                     /protein_id="HGU4547486.1"
                     /translation="MSKQRIFIAGHRGMVGSAIKRQLEQRGDVELVLRTRDELNLLDS
                     RAVHDFFASERIDQVYLAAAKVGGIVANNTYPADFIYQNMMIESNIIHAAHQNDVNKL
                     LFLGSSCIYPKLAKQPMAESELLQGTLEPTNEPYAIAKIAGIKLCESYNRQYGRDYRS
                     VMPTNLYGPHDNFHPSNSHVIPALLRRFHEATAQNAPDVVVWGSGTPMREFLHVDDMA
                     AASIHVMELAHEVWLENTQPMLSHINVGTGVDCTIRELAQTIAKVVGYKGRVVFDASK
                     PDGTPRKLLDVMRLHQLGWYHEISLEAGLASTYQWFLENQDRFRG"
     gene            11450..11929
                     /gene="gmm"
                     /locus_tag="ACHOSC_RS07600"
                     /old_locus_tag="pgaptmp_001520"
                     /gene_synonym="nudD"
                     /gene_synonym="wcaH"
                     /gene_synonym="yefC"
     CDS             11450..11929
                     /gene="gmm"
                     /locus_tag="ACHOSC_RS07600"
                     /old_locus_tag="pgaptmp_001520"
                     /gene_synonym="nudD"
                     /gene_synonym="wcaH"
                     /gene_synonym="yefC"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707946.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-mannose mannosyl hydrolase"
                     /protein_id="HGU4547487.1"
                     /translation="MFLRQEDFATVVRSTPLVSLDFIVENSRGEFLLGKRTNRPAQGY
                     WFVPGGRVQKDETLEAAFERLTMAELGLRLPITAGQFYGVWQHFYDDNFSGTDFTTHY
                     VVLGFRFRVAEEDLLLPDEQHDDYRWLTPDALLASDNVHANSRAYFLAEKRAGVPGL"
     gene            11926..13149
                     /gene="wcaI"
                     /locus_tag="ACHOSC_RS07605"
                     /old_locus_tag="pgaptmp_001521"
     CDS             11926..13149
                     /gene="wcaI"
                     /locus_tag="ACHOSC_RS07605"
                     /old_locus_tag="pgaptmp_001521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis fucosyltransferase
                     WcaI"
                     /protein_id="HGU4547488.1"
                     /translation="MKILVYGINYSPELTGIGKYTGEMVEWLAAQGHEVRVITAPPYY
                     PQWQVGENYSAWRYKREEGAATVWRCPLYVPKQPSTLKRLLHLGSFAVSSFFPLMAQR
                     RWKPERIIGVVPTLFCTPGMRLLAKLSGARTVLHIQDYEVDAMLGLGLAGKGKGGKVA
                     QLATAFERSGLHNVDNVSTISRSMMNKAIEKGVAAENVIFFPNWSEIARFQHVADADV
                     DALRNQLGLPDNKKIILYSGNIGEKQGLENVIEAADRLRDEPLIFAIVGQGGGKARLE
                     KMAQQRGLHNMQFFPLQSYDALPALLKMGDCHLVVQKRGAADAVLPSKLTNILAVGGN
                     AVITAEAHTELGELCETFSGIAVCVEPESVEALVAGIRQALLLPKHNTVAREYAERTL
                     DKENVLRQFINDIRG"
     gene            13152..14588
                     /gene="cpsB"
                     /locus_tag="ACHOSC_RS07610"
                     /old_locus_tag="pgaptmp_001522"
     CDS             13152..14588
                     /gene="cpsB"
                     /locus_tag="ACHOSC_RS07610"
                     /old_locus_tag="pgaptmp_001522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0000271 - polysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannose-1-phosphate guanyltransferase"
                     /protein_id="HGU4547489.1"
                     /translation="MAQSKLYPVVMAGGSGSRLWPLSRVLYPKQFLCLKGDLTMLQTT
                     ICRLNGVECESPVVICNEQHRFIVAEQLRQLNKLTENIILEPAGRNTAPAIALAALAA
                     KRHSPESDPLMLVLAADHVIADEDAFRAAVRNAMPYAEAGKLVTFGIVPDLPETGYGY
                     IRRGEVSAGEQDTVAFEVAQFVEKPNLETAQAYVASGEYYWNSGMFLFRAGRYLEELK
                     KYRPDILDACEKAMSAVDPDLDFIRVDEEAFLACPEESVDYAVMERTADAVVVPMDAG
                     WSDVGSWSSLWEISAHTAEGNVCHGDVINHKTENSYVYAESGLVTTVGVKDLVVVQTK
                     DAVLIADRNAVQDVKKVVEQIKADGRHEHRVHREVYRPWGKYDSIDAGDRYQVKRITV
                     KPGEGLSVQMHHHRAEHWVVVAGTAKVTIDGDIKLLGENESIYIPLGATHCLENPGKI
                     PLDLIEVRSGSYLEEDDVVRFADRYGRV"
     gene            14781..16151
                     /gene="cpsG"
                     /locus_tag="ACHOSC_RS07615"
                     /old_locus_tag="pgaptmp_001523"
                     /gene_synonym="manB"
     CDS             14781..16151
                     /gene="cpsG"
                     /locus_tag="ACHOSC_RS07615"
                     /old_locus_tag="pgaptmp_001523"
                     /gene_synonym="manB"
                     /EC_number="5.4.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis phosphomannomutase
                     CpsG"
                     /protein_id="HGU4547490.1"
                     /translation="MKKLTCFKAYDIRGKLGEELNEDIAWRIGRAYGEFLKPKTIVLG
                     GDVRLTSETLKLALAKGLQDAGVDVLDIGMSGTEEIYFATFHLGVDGGIEVTASHNPM
                     DYNGMKLVREGARPISGDTGLRDVQRLAEANDFPPVDETKRGRYQQINLRDAYVDHLF
                     GYINVKNLTPLKLVINSGNGAAGPVVDAIEARFKALGAPVELIKVHNTPDGNFPNGIP
                     NPLLPECRDDTRNAVIKHGADMGIAFDGDFDRCFLFDEKGQFIEGYYIVGLLAEAFLE
                     KNPGAKIIHDPRLSWNTVDVVTAAGGTPVMSKTGHAFIKERMRKEDAIYGGEMSAHHY
                     FRDFAYCDSGMIPWLLVAELVCLKGKTLGELVRDRMAAFPASGEINSKLAQPVEAINR
                     VEQHFSREALAVDRTDGISMTFADWRFNLRSSNTEPVVRLNVESRGDVPLMEARTRTL
                     LTLLNE"
     gene            16287..17681
                     /gene="wcaJ"
                     /locus_tag="ACHOSC_RS07620"
                     /old_locus_tag="pgaptmp_001524"
     CDS             16287..17681
                     /gene="wcaJ"
                     /locus_tag="ACHOSC_RS07620"
                     /old_locus_tag="pgaptmp_001524"
                     /EC_number="2.7.8.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-phosphate glucose
                     phosphotransferase"
                     /protein_id="HGU4547491.1"
                     /translation="MTNLKKRERAKTNASLISMVQRFSDITIMFAGLWLVCEVSGLSF
                     LYMHLLVALITLVVFQMLGGITDFYRSWRGVRAATEFALLLQNWTLSVIFSAGLVAFN
                     NDFDTQLKIWLAWYGLTSIGLVVCRSCIRIGAGWLRNHGYNKRMVAVAGDLAAGQMLM
                     ESFRNQPWLGFEVVGVYHDPKPGGVSNDWAGNLQQLVEDAKAGKIHNVYIAMQMCDGA
                     RVKKLVHQLADTTCSVLLIPDVFTFNILHSRIEEMNGVPVVPLYDTPLSGVNRLLKRA
                     EDIVLATLILLLISPVLCCIALAVKLSSPGPVIFRQTRYGMDGKPIKVWKFRSMKVME
                     NDKVVTQATQNDPRVTKVGNFLRRTSLDELPQFINVLTGGMSIVGPRPHAVAHNEQYR
                     QLIEGYMLRHKVKPGITGWAQINGWRGETDTLEKMEKRVEFDLEYIREWSVWFDIKIV
                     FLTVFKGFVNKAAY"
     gene            17683..19161
                     /gene="wzxC"
                     /locus_tag="ACHOSC_RS07625"
                     /old_locus_tag="pgaptmp_001525"
     CDS             17683..19161
                     /gene="wzxC"
                     /locus_tag="ACHOSC_RS07625"
                     /old_locus_tag="pgaptmp_001525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid undecaprenyl disphosphate flippase
                     WzxC"
                     /protein_id="HGU4547492.1"
                     /translation="MSLREKTISGAKWSAIATVIIIGLGLVQMTVLARIIDNHQFGLL
                     TVSLVIIALADTLSDFGIANSIIQRKEISHLELTTLYWLNVGLGIVACVAVFLLSDAI
                     GDMLNNPDLAPLIKTLSLAFVVIPHGQQFRALMQKELEFNKIGMIETSAVLAGFTFTV
                     VSAHFWPLAMTAILGYLVNSAVRTLLFGYFGRKIYRPALHFSLASVAPNLRFGAWLTA
                     DSIINYLNTNLSTLVLARILGAGVAGGYNLAYNVAVVPPMKLNPIITRVLFPAFAKIQ
                     DDTEKLRVNFYKLLSVVGIINFPALLGLMVVSNNFVPLVFGEKWSSIIPVLQLLCVVG
                     LLRSVGNPIGSLLMAKARVDISFKFNVFKTFLFIPAIVIGGQMAGAIGVTLGFLLVQI
                     INTILSYFVMIKPVLGSSYRQYILSLWLPFYLSLPTLVVSYALGIVLKGQLALGMLLA
                     VQIAAGVLAFVVMIVLSRHPLVVEVKRQFCRSEKMKMLLRAG"
     gene            19307..20587
                     /gene="wcaK"
                     /locus_tag="ACHOSC_RS07630"
                     /old_locus_tag="pgaptmp_001526"
     CDS             19307..20587
                     /gene="wcaK"
                     /locus_tag="ACHOSC_RS07630"
                     /old_locus_tag="pgaptmp_001526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis pyruvyl transferase
                     WcaK"
                     /protein_id="HGU4547493.1"
                     /translation="MKLLILGNHTCGNRGDSAILRGLLDAINILNPHAEVDVMSRYPV
                     SSSWLLNRPVMGDPLFLQMKQHNSAAGVVGRVKKVLRRRYQHQVLLSRVTDSGKLRNI
                     AIAQGFTDFVRLLSGYDAIIQVGGSFFVDLYGVPQFEHALCTFMAKKSLFMIGHSVGP
                     FQDEQFNQLANYVFGHCDALILRESVSLELMKRSNITTSKVEHGVDTAWLVDHHTEDF
                     TASYAVQHWLDVAAQQKTVAITLRELAPFDKRLGTTQQAYEKAFAGVVNRILDEGYQV
                     IALSTCTGIDSYNKDDRMVALNLRQHISDPARYHVVMDELNDLEMGKILGACELTIGT
                     RLHSAIISMNFATPAIAINYEHKSAGIMQQLGLPEMAIDIRHLLDGSLQVMVADTLGQ
                     LPALNTRLNEAVSRERQTGMQMVKSVLEHIREAK"
     gene            20584..21804
                     /gene="wcaL"
                     /locus_tag="ACHOSC_RS07635"
                     /old_locus_tag="pgaptmp_001527"
     CDS             20584..21804
                     /gene="wcaL"
                     /locus_tag="ACHOSC_RS07635"
                     /old_locus_tag="pgaptmp_001527"
                     /EC_number="2.4.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis glycosyltransferase
                     WcaL"
                     /protein_id="HGU4547494.1"
                     /translation="MKVGFFLLKFPLSSETFVLNQITAFIDMGFEVEIVALQKGDTQN
                     THAAWTKYNLAARTRWLQDEPTGKVAKLRHRASQILRGIHRKNTWQALNLKRYGAESR
                     NLILSAICGQVATPFYADVFIAHFGPAGVTAAKLRELGVIRGKIATIFHGIDISSREV
                     LNHYTPEYQQLFRRGDLMLPISDLWAGRLQKMGCPREKIAVSRMGVDMTRFSPRPVKA
                     PATPLEIISVARLTEKKGLHVAIEACRQLKEQGVAFRYRILGIGPWERRLRTLIEQYQ
                     LEDVVEMPGFKPSHEVKAMLDDADVFLLPSVTGADGDMEGIPVALMEAMAVGIPVVST
                     LHSGIPELVEADKSGWLVPENDARALAQRLAAFSQLDTDELAPVVKRAREKVEHDFNQ
                     QVINRELASLLQAL"
     gene            21815..23209
                     /gene="wcaM"
                     /locus_tag="ACHOSC_RS07640"
                     /old_locus_tag="pgaptmp_001528"
     CDS             21815..23209
                     /gene="wcaM"
                     /locus_tag="ACHOSC_RS07640"
                     /old_locus_tag="pgaptmp_001528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis protein WcaM"
                     /protein_id="HGU4547495.1"
                     /translation="MPFKKLSRRTFLTASSVLAFLHTPFARALPARQSVNINDYNPRD
                     WIASFKQAFSEGQTVVVPAGLVCLNINTGIFIPPGKTLHILGSLRGNGRGRFVLQDGS
                     QVTGEEGGSMHNITLDVRGSDCTIKGLAMSGFGPVTQIYIGGKNKRVMRNLTIDNLTV
                     SHANYAILRQGFHNQIIGANITNCKFSDLQGDAIEWNVAINDRDILISDHVIERINCT
                     NGKINWGIGIGLAGSTYDNNYPEDQAVKNFVVANITGSDCRQLIHVENGKHFVIRNIK
                     ACNITPDFSKKAGIDNATVAIYGCDNFVIDNIEMINSAGMLIGYGVIKGKYLSIPQNF
                     RVNNIQLDNTHLAYKLRGIQISAGNAVSFVALTNVEMKRASLELHNKPQHLFMRNIKV
                     MQESSVGPALSMNFDMRKDVRGVFMAKKETLLSLANVHAVNERGQSSVDIDRINHHIV
                     NVEKINFRLPERRE"
     gene            23384..24277
                     /gene="galF"
                     /locus_tag="ACHOSC_RS07645"
                     /old_locus_tag="pgaptmp_001529"
     CDS             23384..24277
                     /gene="galF"
                     /locus_tag="ACHOSC_RS07645"
                     /old_locus_tag="pgaptmp_001529"
                     /EC_number="2.7.7.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UTP--glucose-1-phosphate uridylyltransferase
                     GalF"
                     /protein_id="HGU4547496.1"
                     /translation="MTNLKAVIPVAGLGMHMLPATKAIPKEMLPIVDKPMIQYIVDEI
                     VAAGIKEILLVTHASKNAVENHFDTSYELESLLELRVKRQLLAEVQSICPPGVTIMNV
                     RQGEPLGLGHSILCARPAIGDNPFVVVLPDVVIDDASADPLRYNLAAMIARFNETGRS
                     QVLAKRMPGELSEYSVIQTKEPLDREGKVSRIVEFIEKPDQPQTLDSDIMAVGRYVLS
                     ADIWPELERTQPGAWGRIQLTDAIAELAKKQSVDAMLMTGDSYDCGKKMGYMQAFVKY
                     GLRNLKEGAKFRKGIEKLLSK"
BASE COUNT         6045 a         6142 c         6674 g         5740 t
ORIGIN      
        1 tgaatgacac ttaatataat tcttaaaaat agccaattac cgaattgtta ccttgcctgc
       61 tattccgtta gctgtaacac ttcctcctgc attattggag agccaatatt caattaccat
      121 aatgtcacct tatattcgga agttaatttc cgcaataacc ctttctcatc aacgattgta
      181 tgcagagagg gtttgaaaaa ttaagctaaa tcagtgtatt ggtagctaaa aagccagggg
      241 cggtagcgtg tctggatgcc tgaaagacca gtctgaatta ttctgtcaat agcctgcgga
      301 tgaaatcaat ttttttagga ctgatgccag ttaaattttt attcaattaa ctgcaaggta
      361 aatgtgcatg gcgacattgc tattaatagt gcacaggata attactctgc caaagtgata
      421 aataaacaat gatgaaatcc aaaatgaaat tgatgccatt attggtgtca gtaaccttga
      481 taagcggttg cacagtactt ccgggcagca atatgtcgac gatgggcaaa gacgtcatca
      541 aacagcagga cgctgatttc gatctcgaca aaatggtgaa tgtttatccg ctgaccccgc
      601 gcctgattga ccaattacgc ccacgcccga atgtagcgcg ccccaatatg acgctggaaa
      661 gcgagatcgc gaattatcag tatcgcgtcg ggccggggga cgttcttaat gtcaccgtct
      721 gggatcaccc ggaactcacc acgccagccg gtcagtaccg cagctccagc gacaccggca
      781 actgggtaca gcctgacggc actatgtttt acccgtatat cggcaaggtt cacgtagtcg
      841 ggaaaacgct cgctgaaatc cgcagtgata ttaccgggcg cttagcgacg tacatcgctg
      901 acccgcaggt ggacgttaat atcgccgcct tccgctcgca aaaggcctat atctcaggtc
      961 aggtgaataa atccggtcaa caggcgatca ccaacgtacc actgaccatt ctcgacgcca
     1021 tcaacgccgc aggtggcctg accgacaccg ctgactggcg caacgtggtg ctaacacaca
     1081 atggtcgtga agaacgcatt tctttgcagg cgctgatgca aaacggcgac ctcaatcaga
     1141 accgactgct ttatcccggc gatattctct acgtgccgcg taatgatgat ctgaaagtat
     1201 ttgtgatggg tgaagtgaag aaacagagca ccctgaaaat ggactttagc ggcatgaccc
     1261 tgactgaagc tctgggcaat gccgaaggca tcgacatgac cacctccaac gccagcggca
     1321 tctttgtcat tcgtccgttg aaaggcgagg gcgggcgtaa cggcaagatt gccaatatct
     1381 accagctgga tatgtccgat gcgacgtcgc tggtgatggc gacagaattc cgcctgcaac
     1441 cttatgacgt ggtgtatgtc accaccgccc cggtttcccg ctggaaccgt ctgatcaatc
     1501 agttgctgcc aactattagc ggtgtccgtt acatgacgga tacagccagc gacattcata
     1561 actggtaatc gtcatgttta acaacatctt agttgtctgt gtcggcaata tttgccgttc
     1621 cccgacggcg gaacgcttac tgcaacgtta tcacccggag ctgaaagtgg agtccgctgg
     1681 actcggcgcg ctggtcggta agggcgctga tcctaccgct atcagcgtcg ccgcagaaca
     1741 tcaactgtct ctggaaggtc actgtgcacg gcaaatcagc cgccgcctgt gtcgcaacta
     1801 tgacctgatt ttgaccatgg aaaagcgcca tatcgaacgc ttatgcgaga tggcacctga
     1861 gatgcgcggc aaagtgatgc tgtttggtca ctgggataac gaatgtgaaa tccccgatcc
     1921 gtatcgcaaa agccgggaaa cgtttgcagc ggtgtacaca ttacttgaac ggtctgcccg
     1981 ccagtgggcg caggcattga acgcagagca ggtataagaa tgacagaaaa agtaaaacaa
     2041 catgccgctc cggtaacggg cagtgatgaa atcgatattg gtcgcctggt cggcaccgtc
     2101 attgaagcgc gctggtgggt gattggcatc accgctgtat ttgccctctg tgctgtggtt
     2161 tacactttct tcgccacgcc gatttatagt gccgacgcac tggtacaaat cgaacaaaac
     2221 agcggcaatt cgttagtgca ggacattggc tcggcattag ccaacaaacc gcctgcatcg
     2281 gacgccgaga tccagttgat tcgttcgcgt ctggtgcttg gtaaaacggt ggacgatcta
     2341 gacctcgata ttgcggtgag caaaaacacg ttcccgattt tcggtgcggg ctgggatcgt
     2401 ttgatgggac gtcagaacga gacggtgaaa gtgaccacct ttaaccgccc gaaagagatg
     2461 gcggaccagg tgtttacgct taatgtgctg gacaataaaa actacaccct gagcagtgat
     2521 ggcggcttta gcgcccgtgg gcaagcgggc cagatgctga aaaaagaagg cgtcacgctg
     2581 atggttgaag ccattcacgc ccgcccgggc agtgagttta ccgtcaccaa atactccacg
     2641 ctggggatga tcaatcaact gcaaaacagc ctgacggtaa cggagaacgg caaagacgca
     2701 ggcgtactga gcctgactta taccggtgaa gatcgtgaac agatccgcga cattcttaac
     2761 agcatcgccc gtaactatca ggaacaaaat attgagcgca aatcggcgga agcgtcgaaa
     2821 agcctcgctt tcctcgcgca acagttaccg gaagtacgta gccgcctcga tgttgccgaa
     2881 aacaaactga atgccttccg tcaggataaa gattctgttg atctgccgct ggaagcgaaa
     2941 gcggtgctcg attcgatggt gaacatcgac gcgcagttga acgaactgac ctttaaagag
     3001 gcggaaatct ccaagctgta caccaaagtt caccccgcgt accgcacgct gctggagaaa
     3061 cgtcaggcgc tggaagacga aaaagccaaa cttaatggtc gcgtaacggc gatgccgaaa
     3121 acccagcagg aaattgtccg tctgacccgc gatgtcgaat ctggtcagca ggtctatatg
     3181 caactgctga ataaagagca ggagctgaaa atcaccgaag ccagcaccgt cggcgatgtg
     3241 cgcattgttg acccggcaat cactcagcct ggtgtgctaa aaccgaagaa agggctgatt
     3301 atcctcggcg cgattatcct tggcctgatg ctctctatcg tgggtgtgct gctgcgctcg
     3361 ttgtttaatc gcggcatcga aagcccgcag gtgctggaag aacacggtat cagcgtctat
     3421 gccagcatcc cgttgtcgga atggcagaaa gcgcgcgata gcgtcaaaac catcaaaggg
     3481 attaaacgct ataaacagag ccagctactg gcggtgggga atccaaccga tctggcgatt
     3541 gaagccatcc gcagtcttcg taccagtttg cacttcgcga tgatgcaggc gcagaacaat
     3601 gtgttgatga tgaccggggt tagcccgtca atcggtaaaa cctttgtctg cgccaacctg
     3661 gcggcggtga tcagccagac caataaacgc gtgttgttga tcgactgcga tatgcgcaaa
     3721 ggctacaccc acgagctgtt gggcactaat aacgttaatg gcctgtcgga aattctgatt
     3781 ggtcagggcg atattactac cgctgctaaa ccgacctcta ttgccaaatt tgacctgatc
     3841 ccgcgcggtc aggtgccgcc aaatccttct gaactgttga tgagcgaacg ctttgccgaa
     3901 ctggtgaact gggcgagtaa aaactatgac ctggtgttga ttgatacgcc gccgattctg
     3961 gcagtgaccg atgcggcaat tgttggtcgt catgttggaa ccacgttaat ggtggcgcgt
     4021 tatgcggtca acacattgaa agaagtggaa accagtctga gccgctttga gcaaaatggt
     4081 attccggtga aaggggtgat tctgaactcc atcttccgcc gcgccagcgc gtatcaggat
     4141 tatggctatt acgaatacga atataagtcg gatgcgaaat aacgacgcct gcattcgcac
     4201 cgccccgtag gccggataag gcgctcacgc cgcatccggc aagcaaacca gctcataagc
     4261 cgggagaaca acctatgaaa gacaatccgc tgatctcaat ctatatgcca acctggaacc
     4321 gccaacaact ggcgattcgg gcaataaaat cggttttgcg ccaggactac agcaactggg
     4381 agatgatcat cgtggatgat tgctccacct cctgggaaca actgcaacag tacgtcaccg
     4441 ccctcaacga tccgcgtatc acttacattc acaacgatat taacagcggg gcgtgcgcgg
     4501 tacgtaacca ggcgattatg ctggcgcaag gggaatatat caccgggatt gatgacgatg
     4561 acgaatggac acccaaccgt ctgagcgtct tcctcgccca taaacagcaa ctggtcacac
     4621 acgccttttt gtacgctaac gactacgtat gccagggaga agtttattcc caaccggcaa
     4681 gcttaccact gtatcccaaa tcgccgtact cacgccgcct gttctataaa cgcaatatca
     4741 ttggcaatca ggtctttacc tgggcatggc gtttcaaaga gtgtttgttc gataccgaac
     4801 tgaaagcagc acaggattac gacatcttcc tgcggatggt ggtggagtac ggcgaaccgt
     4861 ggaaagtgga agaggcgacg cagatcctgc atatcaatca cggtgaaatg cagatcacct
     4921 cgtcaccgaa aaaattctcc gggtacttcc acttttaccg caagcacaaa gataaatttg
     4981 accgtgcaag caaaaaatat cagctgttta ccctctacca gatccgcaat aaacgtatga
     5041 cctggcgcac tttgctgacg ctgctgtcgg tgcgtaacgg caaacgtctg gctgatggta
     5101 ttcgggggcg ctaaagatgc tggaagatct gcgtgccaac agctggagtt tacgcccgtg
     5161 ctgcatggtt cttgcctatc gtgttgctca tttttgttcg gtgtggcgca aaaagaacgt
     5221 cctcaacaat ctgtgggcgg ccccgctgct ggtgctgtat cgcattatca ccgaatgctt
     5281 tttcggttat gaaatccagg ccgccgcgac cattggccgc cgctttacta tccatcacgg
     5341 ttacgccgta gtgatcaata aaaacgtggt agcgggggat gatttcacca ttcgccacgg
     5401 cgtcactatc ggcaatcgtg gtgccgataa catggcatgt ccacacattg gcaacggcgt
     5461 cgaactcggt gccaacgtca ttattcttgg tgatatcacg cttggtaaca acgtcaccgt
     5521 gggcgcgggc agcgtggtgc tcgactctgt cccggacaac gcgctggtgg tgggagaaaa
     5581 agcgcgagtg aaggtaatta aatgaatatt ctgcaattta atgtgcgact ggcggaaggc
     5641 ggggcggcag gtgtggcgtt agatctccat cagcgtgcgc tgcaacaggg gctggcgtca
     5701 cattttgtct acggctacgg caaaggcggc aaagagagcg tcagccatca aaactatccg
     5761 caggtcgtca aacatacgcc gcggatgacc gcgatggcga acattgcgtt gtttcgtctg
     5821 tttaatcgcg atctgtttgg caatttcaat gagttatatc gcaccattac ttgcacaccg
     5881 ggtccggtgg tcctgcattt tcatgtgctg cacagctact ggctaaatct taagagcgtg
     5941 gtgcgctttt gcgaaaaggt gaaaaaccac aaaccggatg tcactctggt ctggacgctg
     6001 cacgaccact ggagcgttac cgggcgctgc gcctttactg acggttgtga aggctggaaa
     6061 acgggctgcc agaaatgccc gaccttaaat aattatccgc cggtgaagat tgatcgggcg
     6121 caccagctgg tggcgggcaa acgccagtta ttccgtgaga tgctggcgct gggctgtcag
     6181 tttatttccc ccagccagca tgtggctgac gctttcaata gcctgtacgg tccagggcgt
     6241 tgccggatta tcaataacgg tattgatatg gcaaccgaag cgattctggc ggatctgcct
     6301 ccggtgcgcg aaacccaggg taagccgaaa atcgcggtgg tggcgcatga cctgcgttac
     6361 gacggcaaaa ctaaccagca actggtgcgt gagatgatgg cgctgggcga caaaatcgaa
     6421 ctgcatacct ttggtaagtt ctcgccgttc accgctggca acgtggttaa tcacggcttt
     6481 gaaactgaca agcgcaagtt gatgagcgcg ctcaatcaga tggatgcgct ggtgttcagt
     6541 tctcgcgtcg ataactaccc gctgattttg tgtgaggcgc tatcgattgg cgtgccggtg
     6601 attgccaccc atagcgacgc ggcgcgggaa gtgctgcaaa aatccggcgg taaaaccgtc
     6661 agcgaagaag acgtgctgca actggtgcag ttaagcaaac cggaaatcgc gcaggcgata
     6721 tttggtacca cgctggctga gttcagccaa cgcagccgcg cggcctacag tggacaacag
     6781 atgctggagg agtatgtcaa cttctatcag aatctgtagc tacctgttgt tgccgctgat
     6841 ttatctgctg gttaacgtca aaatcgccca gcttggcgaa agtttcccca tcaccatcgt
     6901 cactttttta cctgtcttgt tactgctgtt tttagagcgc atcagcgtta aaaaattgat
     6961 gattgcctta gggattggtg cgggactcac ggcgtttaac tatctgtttg gtcagtcgct
     7021 ggatgccagt aaatacgtca cttcaactat gctgtttgtc tatattgtga tcattattgg
     7081 catggtgtgg agtattcgtt ttaaaacaat ttcgccacac aaccatcgca agatattacg
     7141 tttcttttat ctggtggtcg ggttggtggt ggcgctggcg gcggtggaga tggcgcaaat
     7201 tatcctcacc ggcggcagca gtattatgga gtcgatttcg aaatatctga tttacagcaa
     7261 cagctatgta ctgaatttca ttaaattcgg cggcaagcgc acgacagcac tttatttcga
     7321 accggcattt ttcgctctgg cattaatctc aatttggctc agcatcaaac agtttggtat
     7381 caaaacgcct aaaaccgatg ctatgattct cgcagggata atattatccg gatcgttttc
     7441 aggggttatg acctttatcc tgttttattt gctggagtgg gcatttcaat atctgaataa
     7501 agaggcgatt aagaaaaagt taccgttagc attgatttct ctggctgtat tcctggttgg
     7561 tgtggtaatc gcgtttcctt atatttccac ccgtctgggc gatttaggta cggaaggatc
     7621 gtcatcgtat tatcgtattg tcggtccgct ggtgatggtc ggttattctt tgacccatat
     7681 tgacggtgta gtcagatttg gctcacttta tgaatatgtc gcatcattcg gaatatttaa
     7741 cggtgcggat gtcggaaaaa ccatagacaa tggtttgtat ctgctgatta tttatttttc
     7801 ctggttcgca gtgtttttat cgctgtggta catggggaaa gtgataaaaa tgatgatcaa
     7861 cgcttttggt gataaccgca attttcgcgt gcaattatat ctttttactc cggtgtcgct
     7921 gttttttacc ggttcgatat ttagcccgga atatgcattt ttaatcgtct gtccgtttat
     7981 tttgcgaaaa gcgttaaata ttacgaggta agaataagaa catgttgctt agcataatca
     8041 ctgtcgcgtt tcgtaacctc gaagggatag tcaaaacaca tgcctcgctg gcgcatctgg
     8101 cgcaggcgga agatatcagc ttcgaatgga ttgttgtcga tggcggttcc aatgacggca
     8161 cccgtgagta tctggaaaat ctcaatggta tctataacct acgctttgtc agcgagccag
     8221 ataacggtat ctacgacgcc atgaacaaag gcattacgat ggcacaaggc aagttcgcgt
     8281 tgtttctcaa ttcgggcgat atttttcatc aggatgccgc aaattttgtc cgtaagttaa
     8341 aaatgcaaaa agataacatg atgatcaccg gcgatgcgct gctggatttt ggcgacgggc
     8401 ataaaattaa acgtagcgcc aaaccgggct ggtatattta tcacagcctg cccgccagtc
     8461 atcaggcgat atttttccct gtatccggct tgaaaaaatg gcgttatgac ctggaatata
     8521 aagtttcttc cgactacgcg ctggcagcca aaatgtataa agccggttat gcatttaaaa
     8581 aactcaatgg cctggtgtct gaattttcca tgggtggggt atctaccacc aataatatgg
     8641 aattgtgtgc tgacgcgaaa aaagtccaac gacaaatatt acatgtacct ggcttttggg
     8701 ctgaattatc ctggcattta cgccaacgta ctacctcaaa gacgaaagcc ttatataaca
     8761 aagtctgaat ataaggagaa ccaatgcaag atttaagcgg tttctcggtg ccgaaagggt
     8821 tccggggcgg caacgctatt aaagtgcaat tatggtgggc agtacaggca acaatatttg
     8881 cctggtcgcc acaagtattg tatcgctggc gggctttttt attacgttta ttcggagcaa
     8941 aaataggaaa aaacgtagtt attcgtccgt cagtaaaaat tacctatccg tggaaattaa
     9001 ccttaggtga ttacgcgtgg gtcggcgatg acgtcaattt atataccctc ggtgaaataa
     9061 ccattggcgc acattcggtg atatcgcaaa aaagttattt atgcaccggt agccacgacc
     9121 atgcaagtca acatttcacc attaacgcca cgcctattgt gattggcgag aaatgctggc
     9181 tggcaaccga tgtctttgtt gccccaggcg tcacaatcgg caacggcacc gtcgtgggtg
     9241 cacgaagcag tgtttttaaa tcgcttccgg caaatgtggt ttgccggggg aatcccgcag
     9301 tggtgatacg cgaacgcgtt gaaactgaat aaattcaaaa atacagagga ataagacatg
     9361 tcaaaagtcg ctctcatcac cggtgtaacc ggacaagacg gttcttacct ggcagagttt
     9421 ctgctggaaa aaggttacga ggtgcatggt attaagcgtc gcgcatcgtc attcaacacc
     9481 gagcgcgtgg atcacattta tcaggatccg cacacctgca acccgaaatt ccatctgcat
     9541 tatggcgacc tgagtgatac ctctaacctg acgcgcattt tgcgtgaagt acagccggat
     9601 gaagtgtaca acctgggcgc aatgagccac gttgcggtct cttttgagtc accagaatat
     9661 accgctgacg tcgacgcgat gggtacgctg cgcctgctgg aggcgatccg cttcctcggt
     9721 ctggaaaaga aaactcgttt ctatcaggct tccacctctg aactgtacgg tctggtgcag
     9781 gaaattccgc agaaagaaac cacgccgttc tacccgcgat ctccgtatgc ggtcgccaaa
     9841 ctgtacgcct actggatcac cgttaactac cgcgaatcct acggcatgta cgcctgtaac
     9901 ggtattctct tcaaccatga atccccgcgc cgcggcgaaa ccttcgttac ccgcaaaatc
     9961 acccgcgcaa tcgccaacat cgctcagggg ctggagtcgt gcctgtacct cggcaatatg
    10021 gattccctgc gtgactgggg ccacgccaaa gactacgtaa aaatgcagtg gatgatgctg
    10081 caacaggaac agccagaaga tttcgttatc gcgaccggcg ttcagtactc cgtacgtcag
    10141 ttcgtggaaa tggcggcagc acagctgggc atcaaactgc gctttgaagg cacgggcgtt
    10201 gaagagaagg gcattgtggt ttccgtcacc gggcatgacg cgccgggcgt taaaccgggc
    10261 gatgtgatta tcgccgttga cccgcgttac ttccgtccgg cggaagttga aacgctgctc
    10321 ggcgacccga ccaaagcgca cgaaaaactg ggctggaaac cggaaatcac cctcagagag
    10381 atggtgtctg aaatggtggc taatgacctc gaagcggcga aaaaacactc tctgctgaaa
    10441 tctcacggct acgacgtggc gatcgcgctg gagtcataag catgagtaaa caacgaattt
    10501 ttatcgctgg ccatcgcggg atggtcggtt ccgccatcaa gcgacagctc gaacagcgcg
    10561 gtgatgtgga actggtatta cgcacccgcg acgagctgaa cctgctggac agccgcgccg
    10621 tgcatgattt ctttgccagc gaacgtattg accaggtcta tctggcggcg gcgaaagtgg
    10681 gcggcattgt tgccaacaac acctatccgg cggatttcat ctaccagaac atgatgattg
    10741 agagcaacat cattcacgcc gcgcatcaga acgacgtgaa caaactgctg tttctcggat
    10801 cgtcctgcat ctacccgaaa ctggcaaaac agccgatggc agaaagcgag ttgttgcagg
    10861 gcacgctgga gccgactaac gagccttatg ctattgccaa aatcgccggg atcaaattgt
    10921 gcgaatctta caatcgccag tacggacgag attaccgttc agtcatgccg accaacctgt
    10981 acgggccaca cgacaacttc cacccgagta attcgcatgt gatccctgca ttgctgcgtc
    11041 gcttccacga ggcgacggca cagaatgcgc cggacgtggt ggtgtggggc agcggtacac
    11101 cgatgcgtga attcctgcac gtcgatgata tggcggcggc aagcattcat gtgatggagc
    11161 tggcgcacga agtctggctg gagaacaccc agccgatgct gtcgcacatt aacgtcggca
    11221 cgggcgttga ctgcactatc cgcgagctgg cgcaaaccat cgccaaagtg gtgggttaca
    11281 aaggtcgggt ggtttttgat gccagcaaac cggatggcac accgcgcaaa ctgctggatg
    11341 tgatgcgcct gcatcagctt ggctggtatc acgaaatatc actggaagcg gggcttgcca
    11401 gcacttacca gtggttcctt gagaatcaag accgctttcg ggggtaatga tgtttttacg
    11461 tcaggaagac tttgccacgg tagtgcgctc cacaccgctt gtctctctcg actttattgt
    11521 cgagaacagt cgcggcgagt ttctgcttgg caaaagaacc aaccgcccgg cgcagggtta
    11581 ctggtttgtg ccgggagggc gcgtgcagaa agacgaaacg ctggaagccg catttgagcg
    11641 gctgacgatg gcggaactgg ggctgcgtct gccgataaca gcaggccagt tttacggtgt
    11701 ctggcagcac ttttatgacg ataacttctc cggcacggat ttcaccactc actatgtggt
    11761 gctcggtttt cgcttcagag tcgctgaaga agatctgtta ctgccggatg agcagcatga
    11821 cgattaccgc tggctgacgc cggacgcgct gctcgccagt gacaatgttc acgctaacag
    11881 ccgcgcctat tttctcgctg agaagcgtgc cggagtaccc ggattatgaa aatactggtc
    11941 tacggcatta actactcgcc ggagttaacc ggcatcggca aatacaccgg cgagatggtg
    12001 gaatggctgg cggcacaagg tcatgaggtg cgggtcatta ccgcaccgcc ttactacccg
    12061 cagtggcagg tgggcgagaa ctattccgcc tggcgctaca aacgagaaga gggggccgcc
    12121 acggtgtggc gctgcccgct gtatgtgcca aaacagccga gcaccctgaa acgcctgttg
    12181 catctcggca gttttgccgt cagcagtttt ttcccgctga tggcgcaacg tcgctggaag
    12241 ccggagcgca ttatcggcgt agtgccaacg ctgttttgca cgccgggaat gcgtctgctg
    12301 gcgaaactct ctggcgcgcg taccgtgctg catattcagg attacgaagt agatgccatg
    12361 ctggggctgg gccttgccgg aaaaggcaaa ggcggcaaag tggcacagct ggcaacggcg
    12421 ttcgaacgta gcggactaca taacgtcgat aacgtctcca cgatttcgcg ttcgatgatg
    12481 aataaagcca tcgaaaaagg cgtggcggcg gaaaacgtca tcttcttccc caactggtcg
    12541 gaaatcgccc gttttcagca tgttgcagat gccgatgttg atgcccttcg taaccagctt
    12601 ggcctgccgg ataacaaaaa aatcattctt tactccggca atattggtga aaagcagggg
    12661 ctggaaaacg ttattgaagc tgccgatcgc ctgcgcgatg aaccactgat ttttgccatt
    12721 gtcgggcagg gtggcggcaa agcgcgactg gaaaaaatgg cgcagcagcg tggactgcac
    12781 aacatgcaat ttttcccgct gcaatcgtat gacgctttac ccgcactgct gaagatgggc
    12841 gattgccatc tggtggtgca aaaacgcggc gcggcagatg ccgtattgcc gtcgaaactg
    12901 accaatattc tggcggtagg cggtaacgcg gtgattaccg cagaggccca cacagaactg
    12961 ggggagcttt gcgaaacctt ttcgggcatt gcggtttgcg tagaaccgga atcggtcgag
    13021 gcgctggtgg cggggatccg tcaggcgctc ctgctgccca aacacaacac ggtggcacgt
    13081 gaatatgccg aacgcacgct cgataaagag aacgtgttac gtcaatttat aaatgatatt
    13141 cggggataat tatggcgcag tcgaaactct atccagttgt gatggcaggt ggctccggta
    13201 gccgcttatg gccgctttcc cgcgtacttt accccaagca gtttttatgc ctgaaaggcg
    13261 atctcaccat gctgcaaacc accatctgcc gcctgaacgg cgtggagtgc gaaagcccgg
    13321 tggtgatttg caatgagcag caccgcttta ttgtcgcgga acagctgcgt caactgaaca
    13381 aactcaccga gaacattatt ctcgaaccgg cagggcgtaa cactgcacct gccattgcac
    13441 tggcggcgct ggcggcaaaa cgtcatagcc cggagagcga cccgttaatg ctggtattgg
    13501 cggcggatca tgtgattgcc gatgaagacg cgttccgtgc cgccgtgcgt aatgccatgc
    13561 catatgccga agcgggcaag ctggtgacct tcggcattgt gccggatcta cctgaaaccg
    13621 gttatggcta tattcgtcgc ggtgaagtgt ctgcgggtga gcaggatacg gtggcctttg
    13681 aagtggcgca gtttgtcgaa aaaccgaatc tggaaaccgc ccaggcctat gtggcaagcg
    13741 gcgaatatta ctggaacagc ggcatgttcc tgttccgcgc cggacgctat ctcgaagaac
    13801 tgaaaaaata tcgcccggat atcctcgatg cctgcgaaaa agcgatgagc gccgtcgatc
    13861 cggatctcga ttttattcgc gtggatgaag aagcgtttct cgcctgcccg gaagagtcgg
    13921 tggattacgc ggtcatggaa cgtacggcag atgccgttgt ggtgccgatg gatgcgggct
    13981 ggagcgatgt cggttcctgg tcttcattat gggagatcag cgcccacacc gccgagggca
    14041 acgtttgcca cggcgatgtg attaatcaca aaacagaaaa cagctatgtg tatgccgaat
    14101 ctggcctcgt aaccaccgtc ggggtgaaag atctggtggt agtgcagacc aaagatgcgg
    14161 tgctgattgc cgaccgtaac gcggtgcagg atgtgaaaaa agtggtcgag cagatcaaag
    14221 ccgatggtcg ccatgagcat cgggtacatc gcgaagtgta tcgtccgtgg ggcaaatatg
    14281 actctatcga cgcgggcgac cgctaccagg tgaaacgcat caccgtgaaa ccgggcgaag
    14341 gcttgtcggt acagatgcac catcaccgcg cggaacactg ggtggtggtc gcgggaacgg
    14401 caaaagtcac tattgacggt gatatcaaac tgcttggtga aaacgagtcc atttatattc
    14461 cgctgggggc gacgcactgc ctggaaaacc cggggaaaat tccgctcgat ttaattgaag
    14521 tgcgctccgg ctcttatctc gaagaggatg atgtggtgcg cttcgcggat cgctacggac
    14581 gggtgtaaac gtcgcatcag gcgctgtttg tcggatgcgg cgtgaacgcc ttatccgacc
    14641 tacggttcgg atttgtaggc ctgataagac gcggcagcgt cgcatcaggc aatgaatgcg
    14701 aaaccgcgat gtaaataacg acaaaaacaa aattggccgt ttcggtcagg gccaactatt
    14761 gcctgaaaaa gggtaacgat atgaaaaaat taacctgctt taaagcctat gatattcgcg
    14821 ggaaattagg cgaagaactg aatgaagata ttgcctggcg cattggtcgc gcctatggcg
    14881 aatttctcaa accgaaaacc attgtgttag gcggtgatgt ccgcctcacc agcgaaacct
    14941 taaaactggc gctggcgaaa ggtttacagg atgcgggcgt cgatgtgctg gatattggca
    15001 tgtccggcac cgaagagatc tatttcgcca cgttccatct cggcgtggat ggcggcattg
    15061 aagttaccgc cagccataat ccgatggatt ataacggcat gaagctggtt cgcgaggggg
    15121 ctcgcccgat cagcggagat accggactgc gcgacgtcca gcgtctggct gaagccaacg
    15181 acttccctcc cgtcgatgaa accaaacgcg gtcgctatca gcaaatcaac ctgcgtgacg
    15241 cttacgttga tcacctgttc ggttatatca acgtcaaaaa cctcacgccg ctcaagctgg
    15301 tgatcaactc cgggaacggc gcagcgggtc cggtggtgga cgccattgaa gcccgcttta
    15361 aagccctcgg cgcacccgtg gaattaatca aagtgcacaa cacgccggac ggcaatttcc
    15421 ccaacggtat tcctaaccca ctactgccgg aatgccgcga cgacacccgc aatgcggtca
    15481 tcaaacacgg cgcggatatg ggcattgcct ttgatggcga ttttgaccgc tgcttcctgt
    15541 ttgacgaaaa agggcagttt attgagggtt actacattgt cggcctgctg gcagaagcgt
    15601 tcctcgaaaa aaatcccggc gcgaagatca tccacgatcc tcgtctctcc tggaataccg
    15661 ttgatgtggt gactgccgca ggcggcaccc cggtaatgtc gaaaaccgga cacgccttta
    15721 ttaaagaacg tatgcgcaag gaagacgcca tctacggtgg cgaaatgagc gcccaccatt
    15781 acttccgtga tttcgcttac tgcgacagcg gcatgatccc atggctgctg gtcgccgaac
    15841 tggtgtgcct gaaaggaaaa acgctgggcg aactggtgcg cgaccggatg gcggcgtttc
    15901 cggcaagcgg tgagatcaac agcaaactgg cgcaacccgt tgaggcgatt aaccgcgtgg
    15961 aacagcactt tagccgtgag gcgctggcgg tggatcgcac cgatggcatc agcatgacct
    16021 ttgccgactg gcgctttaat ctgcgctcct ccaacaccga accggtggtg cggttgaatg
    16081 tggaatcgcg cggtgatgta ccgctgatgg aagcgcgaac gcgaactctg ctgacgttgc
    16141 tgaacgagta atgtcattac tttccccctc accctgacgc tctactgcct gcggagagaa
    16201 gtgagcacgg aactttcttc tccccctcgc ccctttgggg agagggccgg ggtgaggggc
    16261 gaggcgacaa tagaacagga acaacgatga caaatctaaa aaagcgcgag cgagcgaaaa
    16321 ccaatgcatc gttaatctct atggtgcaac gcttttcaga tatcaccatc atgtttgccg
    16381 gactatggct ggtttgcgaa gtgagcggac tgtcattcct ctacatgcac ctgttggtgg
    16441 cgttgattac gctggtggtg ttccagatgc tgggcggcat caccgatttt tatcgctcat
    16501 ggcgcggtgt tcgggcagcg acagaatttg ccctgttgct acaaaactgg accttaagcg
    16561 tgattttcag cgccggactg gtggcgttca ataatgattt cgacacgcaa ctgaaaatct
    16621 ggctggcgtg gtatgggctg accagcatcg gactggtggt ttgccgttca tgtattcgca
    16681 ttggcgcggg ctggctgcgt aatcatggct ataacaagcg tatggtcgcc gtagcggggg
    16741 atttagccgc cggacaaatg ctgatggaga gcttccgtaa tcagccgtgg ttagggtttg
    16801 aagtggtggg cgtttaccac gacccgaaac cgggtggcgt ttctaacgac tgggcgggca
    16861 atctgcaaca gctggtcgag gatgcgaaag cgggcaagat tcataacgtc tatatcgcga
    16921 tgcaaatgtg cgacggcgcg cgagtgaaaa aactggtcca tcaactggcg gacactacct
    16981 gttcggtgct gctgatcccc gatgtcttta ccttcaacat tctccattcc cgtattgaag
    17041 agatgaacgg cgtaccagtg gtgccgctgt atgacacgcc gctttccggg gttaaccgcc
    17101 tgctcaaacg tgcagaagac attgtgctgg caacgctgat tctgctgctg atctccccgg
    17161 tgctgtgctg cattgcgttg gcggtgaaac tgagctcacc ggggccggtt attttccgcc
    17221 agactcgcta cggcatggac ggcaagccga tcaaagtgtg gaagttccgt tcgatgaaag
    17281 tgatggagaa cgacaaagtg gtgactcagg cgacgcagaa cgatccgcgc gtcaccaaag
    17341 tggggaactt tctgcgccgc acctcgctgg acgaattgcc gcagtttatc aatgtgctga
    17401 ccggggggat gtcgattgtc ggtccacgtc cgcacgcagt agcgcataac gaacagtatc
    17461 gccagctcat tgaaggctac atgctgcgcc acaaagtgaa accgggcatt accggctggg
    17521 cgcagattaa tggctggcgc ggcgaaaccg acacgctgga gaaaatggaa aaacgcgtcg
    17581 agttcgacct tgagtacatc cgcgaatgga gcgtctggtt cgatatcaaa atcgttttcc
    17641 tgacggtatt caaaggcttc gttaacaaag cggcatattg atatgagctt acgtgaaaaa
    17701 accatcagcg gcgcgaagtg gtcggcgatt gccacggtga tcatcatcgg cctcgggctg
    17761 gtgcaaatga ctgtgctggc gcggattatc gacaaccacc agttcggttt gctgactgtg
    17821 tcgctggtga ttatcgcgct ggcagatacg ctttctgact tcggtatcgc taactccatt
    17881 attcagcgaa aagaaatcag ccaccttgaa ctcaccacgt tgtactggct gaacgtcggg
    17941 ctggggatcg tggcgtgtgt ggcggtattt ttgttgagtg atgccattgg cgacatgctg
    18001 aataacccgg acctggcacc gttgattaaa acattgtcac tagcgtttgt ggtgatcccc
    18061 cacgggcaac agttccgcgc gttgatgcaa aaagagctgg agttcaacaa aatcggcatg
    18121 atcgaaacca gcgcggtgct ggcgggcttt acttttacgg tggttagcgc ccatttctgg
    18181 ccgctggcga tgaccgcgat cctcggttat ctggtcaata gtgcggtgag aacgctgctg
    18241 tttggctact ttggccgcaa aatctatcgc cccgctctgc atttctcgct ggcgtcggtg
    18301 gcaccgaact tacgctttgg tgcctggctg acggcggaca gcatcatcaa ctatctcaac
    18361 accaaccttt caacactcgt gctggcgcgt attctcggcg cgggcgtggc tgggggatac
    18421 aacctggcgt acaacgtggc cgttgtgccg ccgatgaaac tgaacccgat catcacccgc
    18481 gtgttgtttc cggcgtttgc caaaattcag gacgataccg aaaagctgcg cgtcaacttc
    18541 tacaagctgc tgtcggtagt ggggattatc aactttccgg cgctgctggg gctgatggtg
    18601 gtgtcgaata actttgtacc gctggtattt ggcgaaaagt ggagcagcat tattccggtg
    18661 ctacagctgc tgtgtgtggt gggtctgctg cgctccgtgg gtaacccgat tggttcgctg
    18721 ctgatggcga aagcgcgggt cgatatcagc tttaaattca acgtatttaa aacatttctg
    18781 tttattccgg cgattgttat tggtgggcag atggcgggcg caatcggcgt cacgcttggc
    18841 ttcctgctgg tgcagattat caacactatt ctgagctatt tcgtgatgat taaaccggtt
    18901 cttggttcca gttatcgcca gtacatcctg agtttgtggc tgccgtttta tctctcgctg
    18961 ccaacgctgg tggtcagtta tgcgctgggc attgtgctga aagggcaact ggcgctgggg
    19021 atgctgctgg cggtgcaaat agccgcgggt gtgctggcgt ttgtggtgat gattgtgctg
    19081 tcgcgccatc cgctggtggt ggaagtgaag cgtcagtttt gtcgcagcga aaaaatgaaa
    19141 atgcttttac gggcggggtg agttgttgtt ttcggttccg acaacggttg ccgggtgcag
    19201 cgtaaaacgc cttatccgtc ctacggttct gtgctcgcat caggcaaaaa acggcaacga
    19261 acacaacaaa cacagcctca cctggcatca aatttatgag gaaaaaatga aattacttat
    19321 tctgggcaac cacacttgcg gcaatcgtgg cgacagcgcc atcttgcgcg gcttacttga
    19381 tgccatcaac atcctcaatc cacatgccga agtggacgtg atgagccgct atcctgtcag
    19441 ttcttcctgg ctactcaacc gcccggtaat gggcgatccg ctgttcctgc aaatgaaaca
    19501 acacaacagc gcggcgggcg ttgtcgggcg cgttaaaaaa gtcctccgtc gccgctacca
    19561 gcatcaggta ttgctctcac gcgtcaccga cagtggcaag ctgcgtaata tcgccatcgc
    19621 ccagggattc accgacttcg tgcgcctgct gtcaggttac gacgccatta ttcaggtcgg
    19681 cggatcgttt tttgtcgatc tctacggcgt gccacagttt gaacatgcac tttgcacgtt
    19741 tatggcgaaa aagtcgctgt ttatgattgg tcacagtgtt ggcccgttcc aggatgagca
    19801 atttaaccaa ctggcgaact acgtttttgg tcactgcgac gcgctgatcc tgcgcgaatc
    19861 ggtcagcctt gaactgatga aacgcagcaa tatcaccact tcaaaagttg aacatggcgt
    19921 cgataccgcg tggctggtcg atcaccacac agaagacttc accgccagct atgctgttca
    19981 acactggctg gacgttgccg cacaacagaa aacggtggca attaccctgc gcgaactggc
    20041 accgttcgac aaacgtctcg gcaccactca acaagcatat gaaaaagcct ttgccggggt
    20101 ggtcaatcgc attctcgacg aaggctatca ggtgattgcg ctctccacct gtacgggtat
    20161 cgacagctat aacaaagacg accgcatggt ggcgctcaac ctgcgccagc acatcagcga
    20221 tcctgcccgt taccacgtag tgatggatga actcaacgat ctggaaatgg gcaaaattct
    20281 gggggcctgt gaactcacca tcggtacgcg cctgcactcc gccattatct caatgaactt
    20341 tgccaccccg gcgattgcca tcaattatga acataaatcc gccgggatta tgcagcagct
    20401 gggactaccg gagatggcga ttgatatccg tcatttatta gacggcagcc tgcaagtgat
    20461 ggttgcggat accttaggcc agcttccggc actgaacacg cgacttaacg aagccgtcag
    20521 tcgcgaacgt cagacgggaa tgcagatggt gaaatctgtg cttgaacaca tcagggaggc
    20581 gaaatgaagg tcggcttctt tttactgaaa tttccgctgt cgtcggaaac cttcgtcctc
    20641 aatcaaatta ccgcgtttat tgatatgggc tttgaggtgg agattgtcgc gctgcaaaaa
    20701 ggcgacaccc agaacaccca cgcggcatgg acgaaataca accttgccgc cagaacccgt
    20761 tggttacagg acgaacctac gggcaaagtg gcgaaactgc gccaccgtgc cagccagatc
    20821 ttacgcggca ttcatcgtaa aaatacctgg caggcactta acctcaaacg ctatggtgcc
    20881 gagtcgcgaa acctgatttt gtctgccatt tgcggccagg tcgcaacacc attttatgcc
    20941 gatgtcttta tcgctcattt tggccctgcg ggggtaaccg cagcaaaact ccgcgaactg
    21001 ggtgtcattc gcggcaaaat tgccactatc ttccacggca ttgatatctc cagtcgggag
    21061 gtgctcaacc attacactcc cgaatatcag caactgttcc gccgtggcga tctgatgcta
    21121 ccaataagcg atctgtgggc cggaaggttg caaaaaatgg gctgcccgag ggaaaaaatc
    21181 gccgtatcgc gcatgggcgt ggacatgacg cgttttagcc cgcgtccggt gaaagcgccc
    21241 gcaacgccgc tggaaatcat ttccgtcgca cgcttaaccg agaaaaaagg cctgcatgtg
    21301 gcgattgaag cctgccgtca gttgaaagag cagggcgtgg catttcgcta tcgcatcctc
    21361 ggcattggcc cgtgggaacg acgcctgcgc accctcatcg aacaatatca actggaagat
    21421 gtggtagaga tgccgggctt taaaccgagc cacgaagtga aagcgatgct cgacgacgcg
    21481 gatgtcttcc tgttgccatc ggttacaggt gcggatggcg atatggaagg tattccggtg
    21541 gcactaatgg aagcgatggc ggtcggcatt ccggtggttt ctactctgca tagcggaata
    21601 ccggaactgg tggaggccga taaatccggc tggctggtgc ctgagaacga tgctcgcgca
    21661 ctggcgcaac gactggcggc gtttagccaa ctggacaccg acgaactggc tccggtcgtc
    21721 aaacgtgcgc gcgaaaaagt cgaacacgat tttaaccagc aggtgatcaa tcgagaactc
    21781 gccagcttgc tgcaggcttt atagaggaga acgcatgcca tttaaaaaac tctcccgacg
    21841 caccttcctg acggcaagct cggtgcttgc cttcctccat acccctttcg ctcgcgcgct
    21901 tcccgcccga caaagcgtta acattaacga ctacaaccct cgcgactgga tcgcctcatt
    21961 taaacaagcc ttcagcgaag ggcaaacggt cgtcgtgcct gccggattgg tttgcctcaa
    22021 tatcaacacc ggcatcttta tccctcctgg caaaacgtta cacatccttg gaagcctgcg
    22081 cggcaacggc agagggcgat ttgtcttaca ggacggcagc caggtgacag gggaggaggg
    22141 cggcagtatg cataacatca ccctggatgt gcgcggctct gactgcacca tcaaagggct
    22201 ggcgatgagc ggctttggtc cggtaacgca gatttatatc ggcggcaaaa acaaacgggt
    22261 catgcgtaac ctgaccatcg ataacctgac cgttagccac gctaattacg ccatcttacg
    22321 ccagggattt cataaccaga ttatcggtgc caacatcacc aattgtaagt tcagcgactt
    22381 acagggcgac gccattgaat ggaatgtggc gattaacgac cgtgatattt tgatatctga
    22441 ccatgtcatc gagcgcatca actgtactaa cggcaaaatc aattggggca tcggcatagg
    22501 tcttgcggga agcacttatg ataacaacta tccggaagac caggcagtga aaaactttgt
    22561 cgtggcgaac atcacgggat cggattgtcg gcagttgata catgttgaaa atggtaaaca
    22621 ttttgttatt cgtaatatca aagcctgcaa tatcacgccg gatttcagta agaaagcagg
    22681 tattgataac gccacggtcg ctatttacgg ttgtgacaat ttcgtgattg ataatattga
    22741 aatgattaat agcgccggga tgttaatcgg ctatggggta attaaaggca aatatctctc
    22801 gataccgcag aatttccgag tgaataatat tcagctggat aatacccacc ttgcttacaa
    22861 attgcgcggc atccaaatct ccgccgggaa tgctgtctcc tttgtggcgc tgactaacgt
    22921 tgagatgaag cgtgcgtcgc tggagttaca caacaagccg caacaccttt ttatgcgtaa
    22981 tatcaaagtg atgcaggaat cctctgttgg acccgcattg agcatgaact tcgacatgcg
    23041 caaagacgtt cgcggcgtgt ttatggcgaa aaaagaaaca ctgctatctc ttgcaaatgt
    23101 tcatgcggtg aatgaaagag ggcaaagctc cgtcgatatc gacaggataa atcaccatat
    23161 tgttaatgtg gaaaagatta actttagatt gccggaacgg agggagtaga tttgcgacca
    23221 ttcctggaaa aatggagcca tacttaggaa caatgctact gcaatctaca acgaagcggc
    23281 gtaacatcac aagtaattca gtaatcaatt cagggtaatt gatgctggcg aaaaaaatcg
    23341 aacaagctat aattcagcaa ccattttaca ggtggatgaa ataatgacga atttaaaagc
    23401 agttattcct gtagcgggtc ttgggatgca tatgttgcct gccactaagg cgattcccaa
    23461 agagatgcta ccgatcgtcg acaagccaat gattcagtac attgttgacg agattgtggc
    23521 tgcagggatc aaagaaatcc tcctggtaac tcatgcgtcc aagaacgcgg tcgaaaacca
    23581 cttcgacacc tcttatgaat tagaatctct tcttgaactg cgcgtgaagc gtcaactgct
    23641 ggcggaagta cagtccatct gtccgccagg cgtgaccatt atgaacgtgc gtcagggcga
    23701 acctttaggt ttaggccact ccattttgtg tgcacgaccc gccattggtg acaatccatt
    23761 tgtcgtggta ctgccggacg ttgtgatcga cgacgccagt gccgacccgc tgcgctacaa
    23821 ccttgctgcc atgattgcgc gtttcaatga aacgggccgt agccaggtgc tggcaaaacg
    23881 tatgccgggt gagctctctg aatactctgt catccagacc aaagaaccgc tggatcgcga
    23941 aggtaaagtc agccgcattg ttgaatttat cgaaaaaccg gatcagccac aaacgctgga
    24001 ctcagacatc atggccgttg gtcgctatgt actttctgcc gatatttggc cggaactgga
    24061 acgtactcag cctggtgcat ggggacgtat tcaactgact gatgctattg ccgagctggc
    24121 gaaaaaacag tccgttgatg ccatgctgat gactggggac agttacgact gcggtaaaaa
    24181 aatgggctat atgcaggcgt tcgtgaagta tggattacga aacctgaaag aaggggcgaa
    24241 gttccgtaaa ggcattgaga agctgttaag caaataatga aaatctgacc ggatgtaacg
    24301 gttgataaga aaattataac ggcagtgaag attcgtgatg aaagtaattt gttgcgaata
    24361 ttcctgccgt tgttttatat aaacaatcag aataacaacg agttagcaat aggattttag
    24421 tcaaagtttt ccaggatttt ccttgtttcc agagcggatt ggtaagacaa ttagcgtttg
    24481 aatttttcgg gtttagcgcg agtgggtaac gctcgtcaca tcgtagacat gcatgcagtg
    24541 cactggtagc tgtaaagcca ggggcggtag cgtgcattaa tacccctatt aatcaaccta
    24601 a
//
LOCUS       DAZKHF020000047        24462 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2201S31241
            SAMN43267800-rid25106413.denovo.047, whole genome shotgun sequence.
ACCESSION   DAZKHF020000047 DAZKHF020000000
VERSION     DAZKHF020000047.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267800
            Sequence Read Archive: SRR30307263
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 24462)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24462)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 24462)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350581.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350581.2
            Genome Coverage       :: 151x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:21:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,652
            CDSs (total)                      :: 5,557
            Genes (coding)                    :: 5,320
            CDSs (with protein)               :: 5,320
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 237
            CDSs (without protein)            :: 237
            Pseudo Genes (ambiguous residues) :: 0 of 237
            Pseudo Genes (frameshifted)       :: 102 of 237
            Pseudo Genes (incomplete)         :: 130 of 237
            Pseudo Genes (internal stop)      :: 52 of 237
            Pseudo Genes (multiple problems)  :: 41 of 237
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24462
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267800-rid25106413.denovo.047"
                     /serotype="O 26:H11"
                     /isolate="2201S31241"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-01"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            complement(94..915)
                     /gene="bacA"
                     /locus_tag="ACHOSC_RS07650"
                     /old_locus_tag="pgaptmp_001530"
     CDS             complement(94..915)
                     /gene="bacA"
                     /locus_tag="ACHOSC_RS07650"
                     /old_locus_tag="pgaptmp_001530"
                     /EC_number="3.6.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708867.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0050380 - undecaprenyl-diphosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0016311 - dephosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-diphosphate phosphatase"
                     /protein_id="HGU4547497.1"
                     /translation="MSDMHSLLIAAILGVVEGLTEFLPVSSTGHMIIVGHLLGFEGDT
                     AKTFEVVIQLGSILAVVVMFWRRLFGLIGIHFGRPLQHEGESKGRLTLIHILLGMIPA
                     VVLGLLFHDTIKSLFNPINVMYALVVGGLLLIAAECLKPKEPRAPGLDDMTYRQAFMI
                     GCFQCLALWPGFSRSGATISGGMLMGVSRYAASEFSFLLAVPMMMGATALDLYKSWGF
                     LTTGDIPMFAVGFITAFVVALIAIKTFLQLIKRISFIPFAIYRFIVAAAVYVVFF"
     gene            complement(1005..1373)
                     /gene="folB"
                     /locus_tag="ACHOSC_RS07655"
                     /old_locus_tag="pgaptmp_001531"
     CDS             complement(1005..1373)
                     /gene="folB"
                     /locus_tag="ACHOSC_RS07655"
                     /old_locus_tag="pgaptmp_001531"
                     /EC_number="4.1.2.25"
                     /EC_number="5.1.99.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708868.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004150 - dihydroneopterin aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0006760 - folic acid-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional dihydroneopterin
                     aldolase/7,8-dihydroneopterin epimerase"
                     /protein_id="HGU4547498.1"
                     /translation="MDIVFIEQLSVITTIGVYDWEQTIEQKLVFDIEMAWDNRKAAKS
                     DDVADCLSYADIAETVVSHVEGARFALVERVAEEVAELLLARFNSPWVRIKLSKPGAV
                     ARAANVGVIIERGNNLKENN"
     gene            1478..2095
                     /gene="plsY"
                     /locus_tag="ACHOSC_RS07660"
                     /old_locus_tag="pgaptmp_001532"
     CDS             1478..2095
                     /gene="plsY"
                     /locus_tag="ACHOSC_RS07660"
                     /old_locus_tag="pgaptmp_001532"
                     /EC_number="2.3.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0008374 - O-acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006654 - phosphatidic acid biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate 1-O-acyltransferase PlsY"
                     /protein_id="HGU4547499.1"
                     /translation="MSAIAPGMILIAYLCGSISSAILVCRLCGLPDPRTSGSGNPGAT
                     NVLRIGGKGAAVAVLIFDVLKGMLPVWGAYELGVSPFWLGLIAIAACLGHIWPVFFGF
                     KGGKGVATAFGAIAPIGWDLTGVMAGTWLLTVLLSGYSSLGAIVSALIAPFYVWWFKP
                     QFTFPVSMLSCLILLRHHDNIQRLWRRQETKIWTKFKRKREKDPE"
     gene            complement(2108..3040)
                     /gene="ttdR"
                     /locus_tag="ACHOSC_RS07665"
                     /old_locus_tag="pgaptmp_001533"
                     /gene_synonym="dan"
     CDS             complement(2108..3040)
                     /gene="ttdR"
                     /locus_tag="ACHOSC_RS07665"
                     /old_locus_tag="pgaptmp_001533"
                     /gene_synonym="dan"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-tartrate utilization transcriptional activator
                     TtdR"
                     /protein_id="HGU4547500.1"
                     /translation="MLNSWPLAKDLQVLVEIVHSGSFSAAAATLGQTPAFVTKRIQIL
                     ENTLATTLLNRSARGVALTESGQRCYEHALEILTQYQRLVDDVTQIKTRPEGMIRIGC
                     SFGFGRSHIAPAITELMRNYPELQVHFELFDRQIDLVQDNIDLDIRINDEIPDYYIAH
                     LLTKNKRILCAAPEYLQKYPQPQSLQELSRHDCLVTKERDMTHGIWELGNGQEKKSVK
                     VSGHLSSNSGEIVLQWALEGKGIMLRSEWDVLPFLESGKLVQVLPEYAQSANIWAVYR
                     EPLYRSMKLRVCVEFLAAWCQQRLGKPDEGYQVM"
     gene            3247..4158
                     /gene="ttdA"
                     /locus_tag="ACHOSC_RS07670"
                     /old_locus_tag="pgaptmp_001534"
     CDS             3247..4158
                     /gene="ttdA"
                     /locus_tag="ACHOSC_RS07670"
                     /old_locus_tag="pgaptmp_001534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008730 - L(+)-tartrate dehydratase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L(+)-tartrate dehydratase subunit alpha"
                     /protein_id="HGU4547501.1"
                     /translation="MMSESNKQQAVNKLTEIVANFTAMISTRMPDDVVDKLKQLKDAE
                     TSSMGKIIYHTMFDNMQKAIDLNRPACQDTGEIMFFVKVGSRFPLLGELQSILKQAVE
                     EATVKAPLRHNAVEIFDEVNTGKNTGSGVPWVTWDIIPDNDDAEIEVYMAGGGCTLPG
                     RSKVLMPSEGYEGVVKFVFENISTLAVNACPPVLVGVGIATSVETAAVLSRKAILRPI
                     GSRHPNPKAAELELRLEEGLNRLGIGPQGLTGNSSVMGVHIESAARHPSTIGVAVSTG
                     CWAHRRGTLLVHADLTFENLSHTRSAL"
     gene            4155..4760
                     /gene="ttdB"
                     /locus_tag="ACHOSC_RS07675"
                     /old_locus_tag="pgaptmp_001535"
     CDS             4155..4760
                     /gene="ttdB"
                     /locus_tag="ACHOSC_RS07675"
                     /old_locus_tag="pgaptmp_001535"
                     /EC_number="4.2.1.32"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016836 - hydro-lyase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L(+)-tartrate dehydratase subunit beta"
                     /protein_id="HGU4547502.1"
                     /translation="MKKILTTPIKAEDLQDIRVGDVIYLTGTLVTCRDVCHRRLIELK
                     RPIPYDLNGKAIFHAGPIVRKNGDKWEMVSVGPTTSMRMESFEREFIEQTGVKLVVGK
                     GGMGPLTEEGCQKFKALHVIFPAGCAVLAATQVEEIEEVHWTELGMPESLWVCRVKEF
                     GPLIVSIDTHGNNLIAENKKLFAERRDPIVEEICEHVHYIK"
     gene            4808..6271
                     /gene="ttdT"
                     /locus_tag="ACHOSC_RS07680"
                     /old_locus_tag="pgaptmp_001536"
     CDS             4808..6271
                     /gene="ttdT"
                     /locus_tag="ACHOSC_RS07680"
                     /old_locus_tag="pgaptmp_001536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-tartrate/succinate antiporter"
                     /protein_id="HGU4547503.1"
                     /translation="MKPSTEWWRYLAPLAVIAIIALLPVPAGLENHTWLYFAVFTGVI
                     VGLILEPVPGAVVAMVGISIIAILSPWLLFSPEQLAQPGFKFTAKSLSWAVSGFSNSV
                     IWLIFAAFMFGTGYEKTGLGRRIALFLVKKMGHRTLFLGYAVMFSELILAPVTPSNSA
                     RGAGIIYPIIRNLPPLYQSQPNDSSSRSIGSYIMWMGIVADCVTSAIFLTAMAPNLLL
                     IGLMKSASHATLSWGDWFLGMLPLSILLVLLVPWLAYVLYPPVLKSGDQVPRWAETEL
                     QAMGPLCSREKRMLGLMVGALVLWIFGGDYIDAAMVGYSVVALMLLLRIISWDDIVSN
                     KAAWNVFFWLASLITLATGLNNTGFISWFGKLLAGSLSGYSPTMVMVALIVVFYLLRY
                     FFASATAYTSALAPMMIAAALAMPEIPLPVFCLMVGAAIGLGSILTPYATGPSPIYYG
                     SGYLPTVDYWRLGAIFGLIFLVLLVITGLLWMPVVLL"
     gene            complement(6314..7327)
                     /gene="tsaD"
                     /locus_tag="ACHOSC_RS07685"
                     /old_locus_tag="pgaptmp_001537"
     CDS             complement(6314..7327)
                     /gene="tsaD"
                     /locus_tag="ACHOSC_RS07685"
                     /old_locus_tag="pgaptmp_001537"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001264383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0002949 - tRNA threonylcarbamoyladenosine
                     modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     transferase subunit TsaD"
                     /protein_id="HGU4547504.1"
                     /translation="MRVLGIETSCDETGIAIYDDEKGLLANQLYSQVKLHADYGGVVP
                     ELASRDHVRKTVPLIQAALKESGLTAKDIDAVAYTAGPGLVGALLVGATVGRSLAFAW
                     NVPAIPVHHMEGHLLAPMLEDNPPEFPFVALLVSGGHTQLISVTGIGQYELLGESIDD
                     AAGEAFDKTAKLLGLDYPGGPLLSKMAAQGTAGRFVFPRPMTDRPGLDFSFSGLKTFA
                     ANTIRDNGTDDQTRADIARAFEDAVVDTLMIKCKRALDQTGFKRLVMAGGVSANRTLR
                     AKLAEMMKKRRGEVFYARPEFCTDNGAMIAYAGMVRFKAGATADLGVSVRPRWPLAEL
                     PAA"
     gene            7565..7780
                     /gene="rpsU"
                     /locus_tag="ACHOSC_RS07690"
                     /old_locus_tag="pgaptmp_001538"
     CDS             7565..7780
                     /gene="rpsU"
                     /locus_tag="ACHOSC_RS07690"
                     /old_locus_tag="pgaptmp_001538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S21"
                     /protein_id="HGU4547505.1"
                     /translation="MPVIKVRENEPFDVALRRFKRSCEKAGVLAEVRRREFYEKPTTE
                     RKRAKASAVKRHAKKLARENARRTRLY"
     gene            7891..9636
                     /gene="dnaG"
                     /locus_tag="ACHOSC_RS07695"
                     /old_locus_tag="pgaptmp_001539"
     CDS             7891..9636
                     /gene="dnaG"
                     /locus_tag="ACHOSC_RS07695"
                     /old_locus_tag="pgaptmp_001539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003896 - DNA primase activity [Evidence
                     IEA];
                     GO_process: GO:0006269 - DNA replication, synthesis of
                     primer [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA primase"
                     /protein_id="HGU4547506.1"
                     /translation="MAGRIPRVFINDLLARTDIVDLIDARVKLKKQGKNFHACCPFHN
                     EKTPSFTVNGEKQFYHCFGCGAHGNAIDFLMNYDKLEFVETVEELAAMHNLEVPFEAG
                     SGPSQIERHQRQTLYQLMDGLNTFYQQSLQQPVATSARQYLEKRGLSHEVIARFAIGF
                     APPGWDNVLKRFGGNPENRQSLIDAGMLVTNDQGRSYDRFRERVMFPIRDKRGRVIGF
                     GGRVLGNDTPKYLNSPETDIFHKGRQLYGLYEAQQDNAEPNRLLVVEGYMDVVALAQY
                     GINYAVASLGTSTTADHIQLLFRATNNVICCYDGDRAGRDAAWRALETALPYMTDGRQ
                     LRFMFLPDGEDPDTLVRKEGKEAFEARMEQAMPLSAFLFNSLMPQVDLSTPDGRARLS
                     TLALPLISQVPGETLRIYLRQELGNKLGILDDSQLERLMPKAAESGVSRPVPQLKRTT
                     MRILIGLLVQNPELATLVPPLENLDENKLPGLGLFRELVNTCLSQPGLTTGQLLEHYR
                     GTNNAATLEKLSMWDDIADKNIAEQTFTDSLNHMFDSLLELRQEELIARERTHGLSNE
                     ERLELWTLNLELAKK"
     gene            9831..11672
                     /gene="rpoD"
                     /locus_tag="ACHOSC_RS07700"
                     /old_locus_tag="pgaptmp_001540"
     CDS             9831..11672
                     /gene="rpoD"
                     /locus_tag="ACHOSC_RS07700"
                     /old_locus_tag="pgaptmp_001540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoD"
                     /protein_id="HGU4547507.1"
                     /translation="MEQNPQSQLKLLVTRGKEQGYLTYAEVNDHLPEDIVDSDQIEDI
                     IQMINDMGIQVMEEAPDADDLMLAENTADEDAAEAAAQVLSSVESEIGRTTDPVRMYM
                     REMGTVELLTREGEIDIAKRIEDGINQVQCSVAEYPEAITYLLEQYDRVEAEEARLSD
                     LITGFVDPNAEEDLAPTATHVGSELSQEDLDDDEDEDEEDGDDDSADDDNSIDPELAR
                     EKFAELRAQYVVTRDTIKAKGRSHAAAQEEILKLSEVFKQFRLVPKQFDYLVNSMRVM
                     MDRVRTQERLIMKLCVEQCKMPKKNFITLFTGNETSDTWFNAAIAMNKPWSEKLHDVS
                     EEVHRALQKLQQIEEETGLTIEQVKDINRRMSIGEAKARRAKKEMVEANLRLVISIAK
                     KYTNRGLQFLDLIQEGNIGLMKAVDKFEYRRGYKFSTYATWWIRQAITRSIADQARTI
                     RIPVHMIETINKLNRISRQMLQEMGREPTPEELAERMLMPEDKIRKVLKIAKEPISME
                     TPIGDDEDSHLGDFIEDTTLELPLDSATTESLRAATHDVLAGLTAREAKVLRMRFGID
                     MNTDHTLEEVGKQFDVTRERIRQIEAKALRKLRHPSRSEVLRSFLDD"
     gene            complement(11751..12257)
                     /gene="mug"
                     /locus_tag="ACHOSC_RS07705"
                     /old_locus_tag="pgaptmp_001541"
     CDS             complement(11751..12257)
                     /gene="mug"
                     /locus_tag="ACHOSC_RS07705"
                     /old_locus_tag="pgaptmp_001541"
                     /EC_number="3.2.2.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000700 - mismatch base pair DNA
                     N-glycosylase activity [Evidence IEA];
                     GO_process: GO:0006285 - base-excision repair, AP site
                     formation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="G/U mismatch-specific DNA glycosylase"
                     /protein_id="HGU4547508.1"
                     /translation="MVEDILAPGLRVVFCGINPGLSSAGTGFPFAHPANRFWKVIYQA
                     GFTDRQLKPQEAQHLLDYRCGVTKLVDRPTVQANEVSKQELHAGGRKLIEKIEDYQPQ
                     ALAILGKQAYEQGFSQRGAQWGKQTLTIGSTQIWVLPNPSGLSRVSLEKLVEAYRELD
                     QALVVRGR"
     gene            12382..12457
                     /locus_tag="ACHOSC_RS07710"
                     /old_locus_tag="pgaptmp_001542"
     tRNA            12382..12457
                     /locus_tag="ACHOSC_RS07710"
                     /old_locus_tag="pgaptmp_001542"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:12415..12417,aa:Ile,seq:cat)
     gene            complement(12511..13275)
                     /gene="nfeF"
                     /locus_tag="ACHOSC_RS07715"
                     /old_locus_tag="pgaptmp_001543"
                     /gene_synonym="yqjH"
     CDS             complement(12511..13275)
                     /gene="nfeF"
                     /locus_tag="ACHOSC_RS07715"
                     /old_locus_tag="pgaptmp_001543"
                     /gene_synonym="yqjH"
                     /EC_number="1.16.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent ferric chelate reductase NfeF"
                     /protein_id="HGU4547509.1"
                     /translation="MNNSPRYPQRVRNDLRFRELTVLRVERISAGFQRIVLGGEALDG
                     FTSRGFDDHSKLFFPQPDAHFVPPTVTEEGIVWPEGPRPLSRDYTPLYDELRHELAID
                     FFIHDGGVASGWAMQAQPGDKLTVAGPRGSLVVPEDYAYQLYVCDESGMPALRRRLET
                     LSKLAVKPQVSALVSVRDNACQDYLAHLDGFNIEWLAHDEQAVDARLAQMQIPADDYF
                     IWITGEGKVVKNLSRRFEAEQYDPQRVRAAAYWHAK"
     gene            13552..14175
                     /gene="nfeR"
                     /locus_tag="ACHOSC_RS07720"
                     /old_locus_tag="pgaptmp_001544"
                     /gene_synonym="yqjI"
     CDS             13552..14175
                     /gene="nfeR"
                     /locus_tag="ACHOSC_RS07720"
                     /old_locus_tag="pgaptmp_001544"
                     /gene_synonym="yqjI"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator NfeR"
                     /protein_id="HGU4547510.1"
                     /translation="MSHHHEGCCKHEGQPRHEGCCKGEKSEHEHCGHGHQHEHGQCCG
                     GRHGRGGGRRQRFFGHGELRLVILDILSRDDSHGYELIKAIENLTQGNYTPSPGVIYP
                     TLDFLQEQSLITIREEEGGKKQIALTEQGAQWLEENREQVEMIEERIKARCVGAALRQ
                     NPQMKRALDNFKAVLDLHVNQSDISDAQIKKIIAVIDRAAFDITQLD"
     gene            complement(14329..15849)
                     /gene="aer"
                     /locus_tag="ACHOSC_RS07725"
                     /old_locus_tag="pgaptmp_001545"
     CDS             complement(14329..15849)
                     /gene="aer"
                     /locus_tag="ACHOSC_RS07725"
                     /old_locus_tag="pgaptmp_001545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311981.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aerotaxis sensor receptor Aer"
                     /protein_id="HGU4547511.1"
                     /translation="MSSHPYVTQQNTPLADDTTLMSTTDLQSYITHANDTFVQVSGFT
                     LQELQGQPHNMVRHPDMPKAAFADMWFTLKKGEPWSGIVKNRRKNGDHYWVRANAVPM
                     VREGKISGYMSIRTRATDEEIAAVEPLYKALNAGRTSKRIHKGLVVRKGWLGKLPSLP
                     LRWRARGVMTLMFILLAAMLWFVAAPVVTYFLCVLVVLLASACFEWQIVRPIENVARQ
                     ALKVATGERNSVEHLNRSDELGLTLRAVGQLGLMCRWLINDVSSQVSSVRNGSETLAK
                     GTDELNEHTQQTVDNVQQTVATMNQMAASVKQNSATASAADKLSITASNAAVQGGEAM
                     TTVIKTMDDIADSTQRIGTITSLINDIAFQTNILALNAAVEAARAGEQGKGFAVVAGE
                     VRHLASRSANAANDIRKLIDASADKVQSGSQQVHAAGRTMEDIVAQVKNVTQLIAQIS
                     HSTLEQADGLSSLTRAVDELNLITQKNAELVEESAQVSAMVKHRASRLEDAVTVLH"
     gene            16267..17646
                     /gene="ygjG"
                     /locus_tag="ACHOSC_RS07730"
                     /old_locus_tag="pgaptmp_001546"
     CDS             16267..17646
                     /gene="ygjG"
                     /locus_tag="ACHOSC_RS07730"
                     /old_locus_tag="pgaptmp_001546"
                     /EC_number="2.6.1.82"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417544.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_function: GO:0033094 - putrescine--2-oxoglutarate
                     transaminase activity [Evidence IEA];
                     GO_process: GO:0009447 - putrescine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putrescine aminotransferase"
                     /protein_id="HGU4547512.1"
                     /translation="MNRLPSSASALACSAHALNLIEKRTLDHEEMKALNREVIEYFKE
                     HVNPGFLEYRKSVTAGGDYGAVEWQAGGLNTLVDTQGQEFIDCLGGFGIFNVGHRNPV
                     VVSAVQNQLAKQPLHSQELLDPLRAMLAKTLAALTPGKLKYSFFCNSGTESVEAALKL
                     AKAYQSPRGKFTFIATSGAFHGKSLGALSATAKSTFRKPFMPLLPGFRHVPFGNIEAM
                     RTALNECKKTGDDVAAVILEPIQGEGGVILPPPGYLTAVRKLCDEFGALMILDEVQTG
                     MGRTGKMFACEHENVQPDILCLAKALGGGVMPIGATIATEEVFSVLFDNPFLHTTTFG
                     GNPLACAAALATINVLLEQNLPAQAEQKGDMLLDGFRQLAREYPDLVQEARGKGMLMA
                     IEFVDNEIGYNFASEMFRQRVLVAGTLNNAKTIRIEPPLTLTIEQCELVIKAARKALA
                     AMRVSVEEA"
     gene            complement(17688..18020)
                     /locus_tag="ACHOSC_RS07735"
                     /old_locus_tag="pgaptmp_001547"
     CDS             complement(17688..18020)
                     /locus_tag="ACHOSC_RS07735"
                     /old_locus_tag="pgaptmp_001547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA-binding protein"
                     /protein_id="HGU4547513.1"
                     /translation="METVAYADFARLEMRVGKIVEVKRHENADKLYIVQVDVGEKTLQ
                     TVTSLVPYYSEEELMGKTVVVLCNLQKAKMRGETSECMLLCAETDDGSESVLLTPERM
                     MPAGVRVV"
     gene            18239..19222
                     /gene="ebgR"
                     /locus_tag="ACHOSC_RS07740"
                     /old_locus_tag="pgaptmp_001548"
     CDS             18239..19222
                     /gene="ebgR"
                     /locus_tag="ACHOSC_RS07740"
                     /old_locus_tag="pgaptmp_001548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator EbgR"
                     /protein_id="HGU4547514.1"
                     /translation="MATLKDIAIEAGVSLATVSRVLNDDPTLNVKEETKHRILEIAEK
                     LEYKTSSARKLQTGAVNQHHILAIYSYQQELEINDPYYLAIRHGIETQCEKLGIELTN
                     CYEHSGLPDIKNVTGILIVGKPTPALRAAASALTDNICFIDFHEPGSGYDAVDIDLAR
                     ISKEIIDFYINQGVNRIGFIGGEDEPGKADIREVAFAEYGRLKQVVREEDIWRGGFSS
                     SSGYELAKQMLAREDYPKALFVASDSIAIGVLRAIHERGLNIPQDISLISVNDIPTAR
                     FTFPPLSTVRIHSEMMGSQGVNLVYEKARDGRALPLLVFVPSKLKLRGTTR"
     gene            19406..22498
                     /gene="ebgA"
                     /locus_tag="ACHOSC_RS07745"
                     /old_locus_tag="pgaptmp_001549"
     CDS             19406..22498
                     /gene="ebgA"
                     /locus_tag="ACHOSC_RS07745"
                     /old_locus_tag="pgaptmp_001549"
                     /EC_number="3.2.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311985.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004565 - beta-galactosidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-galactosidase subunit alpha"
                     /protein_id="HGU4547515.1"
                     /translation="MNRWENIQLTHENRLAPRAYFFSYDSVAQARTFARETSSLFLPL
                     SGQWNFHFFDHPLQVPEAFTSELMADWGHITVPAMWQMEGHGKLQYTDEGFPFPIDVP
                     FVPSDNPTGAYQRIFTLSDGWQGKQTLIKFDGVETYFEVYVNGQYVGFSKGSRLTAEF
                     DISAMVKTGDNLLCVRVMQWADSTYVEDQDMWWSAGIFRDVYLVGKHLTHINDFTVRT
                     DFDEAYCDATLSCEVVLENLAASPVVTTLEYTLFDGERVVHSSAIDHLAIEKLTSASF
                     AFTVEQPQQWSAESPYLYHLVMTLKDANGNVLEVVPQRVGFRDIKVRDGLFWINNRYV
                     MLHGVNRHDNDHRKGRAVGMDRVEKDLQLMKQHNINSVRTAHYPNDPRFYELCDIYGL
                     FVMAETDVESHGFANVGDISRITDDPQWEKVYVERIVRHIHAQKNHPSIIIWSLGNES
                     GYGCNIRAMYHAAKALDDTRLVHYEEDRDAEVVDIISTMYTRVPLMNEFGEYPHPKPR
                     IICEYAHAMGNGPGGLTEYQNVFYKHDCIQGHYVWEWCDHGIQAQDDNGNVWYKFGGD
                     YGDYPNNYNFCLDGLIYSDQTPGPGLKEYKQVIAPVKIHALDLTRGELKVENKLWFTT
                     LDDYTLHAEVRVEGETLATQQIKLRDVAPNSEAPLQITLPQLDAREAFLNITVTKDSR
                     TRYSEAGHSIATYQFPLKENTAQPVPFAPNNARPLTLEDDRLSCTVRGYNFAITFSKM
                     SGKPTSWQVNGESLLTREPKINFFKPMIDNHKQENEGLWQPNHLQIMQEHLRDFAVEQ
                     SDGEVLIISRTVIAPPVFDFGMRCTYIWRIAADGQVNVALSGERYGDYPHIIPCIGFT
                     MGINGEYDQVAYYGRGPGENYADSQQANIIDIWRSTVDAMFENYPFPQNNGNRQHVRW
                     TALTNRHGNGLLVVPQRPINFSAWHYTQENIHAAQHCNELQRSDDITLNLDHQLLGLG
                     SNSWGSEVLDSWRVWFRDFSYGFTLLPVSGGEATAQSLASYEFGAGFFSTNLHSENKQ
                     "
     gene            22495..22944
                     /locus_tag="ACHOSC_RS07750"
                     /old_locus_tag="pgaptmp_001550"
     CDS             22495..22944
                     /locus_tag="ACHOSC_RS07750"
                     /old_locus_tag="pgaptmp_001550"
                     /EC_number="3.2.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-galactosidase subunit beta"
                     /protein_id="HGU4547516.1"
                     /translation="MRIIDNLEQFRQIYASGKKWQRCVEAIENIDNIQPGVAHSIGDS
                     LTYRVETDSATDALFTGHRRYFEVHYYLQGQQKIEYAPKETLQVVEYYRDETDREYLK
                     GCGETVEVHEGQIVICDIHEAYRFICNNAVKKVVLKVTIEDGYFHNK"
     gene            23007..24440
                     /locus_tag="ACHOSC_RS07755"
                     /old_locus_tag="pgaptmp_001551"
     CDS             23007..24440
                     /locus_tag="ACHOSC_RS07755"
                     /old_locus_tag="pgaptmp_001551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HGU4547517.1"
                     /translation="MSDTKRNTIGKFGLLSLTFAAVYSFNNVINNNIELGLASAPMFF
                     LATIFYFIPFCLIIAEFVSLNKNSEAGVYAWVKSSLGGRWAFITAYTYWFVNLFFFTS
                     LLPRVIAYASYAFLGYEYIMTPVATTIISMVLFAFSTWVSTNGAKMLGPITSVTSTLM
                     LLLTLSYILLAGTALVGGVQPADPITVDAMIPNFNWAFLGVTTWIFMAAGGAESVAVY
                     VNDVKGGSKSFVKVIILAGIFIGVLYSVSSVLINVFVSSKELKFTVGSVQVFHGMAAY
                     FGLPEALMNRFVGLVSFTAMFGSLLMWTATPVKIFFSEIPEGIFGKKTVELNENGVPA
                     RAAWIQFLIVIPLMIIPMLGSNTVQDLMNTIINMTAAASMLPPLFIMLAYLNLRAKLD
                     HLPRDFRMGSRRTGIIVVSMLIAIFAVGFVASTFPTGANILTIIFYNVGGIVIFLGFA
                     WWKYSKYIKGLTAEERHIEATPASNVD"
BASE COUNT         5912 a         6611 c         6225 g         5714 t
ORIGIN      
        1 agaccagcac cggtgcgaac aatcaccccc accccggccc tctccctaca gagcgaggga
       61 gaaaaccagg cctgctcccg aaccttttct caatcaaaag aacacgacgt acaccgcagc
      121 cgccacaata aagcgataaa tggcgaacgg gataaacgaa atgcgcttaa tcaattgcag
      181 gaaggtttta atcgctatca gcgccaccac aaaagcggtg ataaacccaa cggcaaacat
      241 cgggatatcg ccggttgtca ggaagcccca gcttttgtag agatcgagcg ccgttgcgcc
      301 catcatcatc ggtaccgcca gcaggaacga gaactcggaa gcagcgtaac ggctcacgcc
      361 catcagcatc ccacctgaaa tggtggcccc ggaacgggag aaacccggcc acagcgccag
      421 acactggaaa cagccaatca taaacgcctg acgataggtc atatcatcaa gacccggcgc
      481 acgcggctct ttcggcttca ggcattcggc ggcaatcagc aacaaaccgc caacgaccag
      541 tgcatacatc acatttatcg ggttaaacaa tgacttaatc gtgtcgtgga acaacagccc
      601 cagtactacc gccggaatca tccccagcaa aatgtggatc agcgttaaac gacctttgct
      661 ttctccttcg tgctgcaacg ggcggccaaa gtggatgcca atcaggccaa acagacgccg
      721 ccagaacatc actactaccg ccagaattga tcctaactgg atcacaactt caaaggtttt
      781 cgccgtgtcg ccctcgaacc ccaacaagtg accgacaata atcatatggc ccgtgctgga
      841 taccggcaga aattctgtca atccttcgac cacacccaat attgccgcta tcagcagcga
      901 gtgcatatcg ctcatcaata aacccctaaa ttattaaaat gtaccgcttg tccgaactac
      961 tgcgtatgac caggttataa ccgtttggtt taacagctgt aaaattaatt attttctttc
     1021 agattattgc cacgctcaat gattacgcca acattcgccg cccgcgccac tgcgcctggc
     1081 ttgctgagtt tgatacgcac ccacggcgag ttaaagcgtg ctaacagcag ctccgccacc
     1141 tcttcagcca cgcgttccac cagcgcaaaa cgcgccccct cgacgtggct gaccaccgtt
     1201 tctgcaatgt cagcgtaact gaggcaatcc gccacatcat cacttttcgc cgctttacgg
     1261 ttatcccatg ccatttcgat atcgaacact aacttctgtt cgatggtctg ttcccagtcg
     1321 taaacaccaa tagtggtgat taccgaaagt tgctctataa atacaatatc catcacgtcc
     1381 tgcctgcttt ttggctaacc ggataccact tccggcgaaa tgtgcgtatt atccacagat
     1441 tcatcgttga acacgaattt tcaaaacgga acagcttatg agtgcaatcg cgcctggaat
     1501 gatcctcatc gcgtacctct gcggctccat ttccagtgcc attctggttt gccgcttgtg
     1561 tgggctgccc gatccgcgaa ccagcggctc cggcaatcca ggcgcaacca atgtgttacg
     1621 tatcggtggc aagggagcag ccgtagcagt actgattttc gacgttctga aaggaatgtt
     1681 gcccgtctgg ggcgcgtatg aattaggtgt cagccccttc tggctaggct taattgccat
     1741 cgccgcctgt cttggacaca tctggcccgt tttcttcgga tttaaaggag gaaaaggcgt
     1801 tgctaccgct tttggtgcca tcgcacccat tggctgggat ctcaccggag taatggcggg
     1861 aacctggtta ctgaccgtgc tattgagcgg atactcgtcg ctgggagcga ttgtcagtgc
     1921 actgattgct ccgttttatg tatggtggtt taagccacaa ttcaccttcc cggtttcgat
     1981 gctctcttgc ctgatcctgc tgcgtcatca tgacaacatc cagcgtctgt ggcgtcgtca
     2041 ggagacaaaa atctggacga aattcaaaag aaagcgcgaa aaggatcccg agtgatttct
     2101 ggtggatcta catgacctga tagccttcat cgggcttgcc cagccgttgc tggcaccatg
     2161 ccgccagaaa ttccacgcag acacgtaatt tcatgctgcg atagagcggc tcccggtaaa
     2221 cagcccagat attggcgctt tgtgcatact ccggcaatac ttgcaccagt ttgccactct
     2281 ccagaaacgg caacacatcc cactcggaac gcagcataat ccctttgccc tccagcgccc
     2341 attgcaacac tatctcgccg ctattggagg aaagatgccc gctaaccttc accgattttt
     2401 tctcctgacc gttccccaac tcccatattc cgtgggtcat atcgcgctct ttggtcacca
     2461 gacagtcatg acgacttaat tcctgtaatg attgtggctg cggatatttt tgcagatact
     2521 caggtgctgc gcataatatt cttttatttt tcgttaacag atgcgcaata taataatcag
     2581 gaatttcgtc attaatacga atatccagat caatattatc ctgcactaaa tcaatttgcc
     2641 gatcgaacaa ttcaaaatgc acctgtaact caggataatt gcgcataagt tcggtaatgg
     2701 ctggcgcaat atggctgcgc ccaaaaccaa aactgcaacc aatacgaatc atcccctccg
     2761 ggcgcgtttt gatttgcgtg acgtcatcga ccagtcgctg atattgggtg aggatttcca
     2821 gcgcatgttc gtaacaacgt tggccacttt cagtcagcgc cacgccgcga gcggagcggt
     2881 tcagcagcgt tgttgccagc gtattttcaa ggatttggat ccgcttggtg acaaatgcag
     2941 gcgtctgccc aagcgtcgcc gctgcagcac taaaactccc gctgtggacg atttctacca
     3001 gtacctgcaa gtctttggct aaaggccagc tattcagcat cgttaattat ccgcagttgt
     3061 gataagcgca gtgtatttcg caaaacattg ccattccctg ctctggcgca atattcattc
     3121 ttattttcgc gttgattcac gaactgttaa ttacatattc acggtggcaa aaaatataaa
     3181 accacatttt tagtggtagt ttgtggcggt gaatttttcc agacaaatac aaaaactgga
     3241 gttgccatga tgagcgaaag taataagcaa caggcagtga ataagttgac agagattgtc
     3301 gctaacttta ccgccatgat ttctacccga atgcctgatg acgtggtgga taaactaaaa
     3361 cagctaaagg atgccgaaac gtcgtcgatg gggaaaatta tctaccatac gatgttcgac
     3421 aacatgcaaa aagcgattga cctgaatcgt cctgcctgtc aggacaccgg ggagattatg
     3481 ttcttcgtta aagtcggttc ccgcttccca ctgcttggcg agctgcaaag catactcaaa
     3541 caagccgtgg aagaggcaac cgtcaaagcg ccactacgtc acaatgcggt agaaattttt
     3601 gacgaagtaa acaccggcaa aaataccggt agcggcgtac cgtgggtcac ctgggacatc
     3661 atccccgaca atgacgatgc ggaaatcgaa gtttacatgg caggcggcgg ctgcacgcta
     3721 cctggccgct cgaaagtgtt aatgccgtca gaaggctacg aaggcgtggt gaaattcgtc
     3781 ttcgaaaata tctccaccct cgccgtaaac gcctgtccac cggtactggt gggcgtgggc
     3841 atcgccacct cggtggaaac cgccgccgtg ctctcgcgaa aagccatttt gcgcccgatt
     3901 ggcagccgcc atcccaatcc aaaagcggca gagctggagc tacgcctgga agaaggactc
     3961 aaccgtctgg gaattggccc acaagggctg actggcaaca gttcagtgat gggcgtgcat
     4021 atcgaatctg ccgcccgcca tccgtcaacc atcggcgttg ctgtttctac aggctgctgg
     4081 gcgcatcgtc gcggcacact gctggttcat gccgatctca cctttgaaaa tctgtctcac
     4141 acccggagcg cgttatgaaa aagatcctga caaccccgat caaagctgaa gatctgcaag
     4201 atattcgcgt cggcgatgtg atctacctga ccggtacgct ggtgacctgc cgtgacgttt
     4261 gtcaccgccg cttgatcgaa ctgaaacgtc cgatccctta cgatctcaac ggcaaagcga
     4321 ttttccacgc tggccccatc gtgcgcaaaa acggcgataa atgggagatg gtttccgttg
     4381 gcccgacaac cagtatgcgt atggaaagtt ttgaacgtga atttatcgag cagaccggcg
     4441 tgaaactggt ggttggcaaa ggtggtatgg ggccgctgac cgaagaaggc tgccagaaat
     4501 tcaaggcgct acatgtgatt ttcccggcag gctgcgcggt gctggcggca acccaggtag
     4561 aagagattga agaagtgcac tggacagaac tcgggatgcc ggagtcactg tgggtctgcc
     4621 gggtcaaaga gttcggcccg ctgattgtct ctattgatac ccacggcaac aacctgatag
     4681 ccgaaaacaa aaagctgttc gccgaacgcc gcgatcccat cgtggaagag atctgcgagc
     4741 acgtccatta catcaaataa ccctcccgga gaggctcacc cctctccttt ttcgcaggca
     4801 taacacgatg aaaccttcca ctgaatggtg gcgatatctt gcgccgctgg cggtcatcgc
     4861 cattattgct ctacttccgg ttcccgcagg gctggagaac catacctggc tctactttgc
     4921 cgtttttact ggcgtgatcg ttggactgat cctcgaaccc gtgccgggtg ccgtggtggc
     4981 gatggtgggt atctccatca tcgccatact ctctccctgg ctgctgttca gccccgaaca
     5041 gctcgcccag ccaggcttta aattcactgc aaaatccctc tcgtgggcgg tttccggttt
     5101 ttctaattcg gttatctggc tgattttcgc cgcctttatg tttggcacag gctatgaaaa
     5161 aaccgggctt ggacgccgca ttgcgctgtt tctggtgaaa aagatgggac atcgcacgct
     5221 gtttctcggc tatgcggtga tgttctccga gctgatcctc gcacctgtaa caccgtccaa
     5281 ctcggcgcgt ggtgcgggga ttatctatcc catcatccgt aacctgccac cgctctatca
     5341 atcacaacca aacgacagca gttcgcgcag cattggctcg tacatcatgt ggatggggat
     5401 tgttgccgac tgcgtgacca gcgccatttt cttgacggcg atggcaccaa acttgctgtt
     5461 aattggactg atgaaaagcg catctcacgc cacgctgagt tggggcgact ggttcctcgg
     5521 gatgttgccg ctcagtattt tgctggtgct gctggttccc tggctggctt atgtgctgta
     5581 cccgccggta ctgaagtctg gcgatcaggt gccgcgctgg gcagagacgg aactgcaggc
     5641 aatgggcccg ctctgttcgc gtgaaaaacg gatgctgggg ctgatggtag gcgcgctggt
     5701 gctgtggatt ttcggcggtg attatatcga tgccgcgatg gtcggttaca gcgtggtggc
     5761 actgatgctg cttctgcgca ttatcagttg ggacgacatt gtcagtaata aagcggcgtg
     5821 gaacgttttc ttctggctgg cctcgcttat caccctcgct accggactca acaacactgg
     5881 ttttattagc tggtttggca aactgttagc aggcagctta agcggttatt cgccaacgat
     5941 ggtgatggtg gcgttgattg tggtgtttta tctgctgcgc tactttttcg ccagcgccac
     6001 ggcgtatacc tccgccctcg caccgatgat gattgccgct gcgctggcga tgccggaaat
     6061 cccgctgccg gtgttctgcc tgatggttgg cgcggcaatt ggtctgggga gcattcttac
     6121 accatacgcc accggaccca gtccgattta ctacggtagt ggttatctgc caacggtgga
     6181 ttactggcga ctgggggcga tttttggact gatattcctc gtgttgctgg tgattaccgg
     6241 cttgctgtgg atgcccgtgg tgttgcttta agtgaggacg gtgccggaaa ttgctctccg
     6301 gcaccggata gttttacgca gccggtaact ctgccagtgg ccagcgcgga cgcacgctaa
     6361 cgccgagatc cgccgtcgcg cctgctttaa accgcaccat tccggcatag gcgatcatcg
     6421 cgccgttatc agtacaaaat tccggacgcg cgtagaacac ttcgccgcgg cgttttttca
     6481 tcatttccgc cagcttcgcc cgtaacgtgc ggttagcact cacgccgccc gccatgacca
     6541 gtcgcttaaa gcccgtctga tccaacgctc gcttacactt aatcatcaac gtatcgacca
     6601 ccgcatcttc aaaggcgcgg gcgatatcag cacgcgtctg gtcgtcggtg ccgttgtcac
     6661 gaatcgtgtt cgccgcaaag gttttcagac cagaaaagct gaaatccagc cccggacggt
     6721 cggtcatcgg acgcgggaaa acaaagcgcc cggcagtacc ctgagccgcc attttcgaca
     6781 gtaacggtcc gccaggataa tccagcccca gcagcttcgc ggttttatca aacgcttcac
     6841 cggcggcatc atcgatagac tcgccgagca gctcgtactg accaatgcca gtcacgctga
     6901 ttaactgcgt atgaccgccg gacaccagca gcgcgacaaa cggaaattcc ggcgggttat
     6961 cttccagcat cggcgctaac agatgccctt ccatatggtg taccgggatt gccggaacgt
     7021 tccaggcaaa cgccagagaa cgccccacgg ttgcgccaac cagcagtgcg ccgactaatc
     7081 cagggcctgc ggtataggcc acagcatcaa tgtcttttgc cgttaaacca gactccttta
     7141 gcgccgcctg gatcaacggt acggttttgc gcacgtgatc gcgggaggcc agttcaggca
     7201 cgacgccgcc gtagtcagcg tgcaatttca cctgactata caattggttg gctaacaaac
     7261 ctttttcatc gtcgtaaatg gcgatgccgg tttcatcgca ggaagtttca atacccagta
     7321 cacgcatgac ttgttttacc tcgctttatt accgcgcagt gtaggaccaa tgcgggttga
     7381 tgtaaaactt tgttcgcccc tggagaaagc ctcgtgtata ctcctcaccc ttataaaagt
     7441 ccctttcaaa aaaggccgcg gtgctttaca aagcagcagc aattgcagta aaattccgca
     7501 ccattttgaa ataagctggc gttgatgcca gcggcaaacc gaattaatca aaggtgagag
     7561 gcacatgccg gtaattaaag tacgtgaaaa cgagccgttc gacgtagctc tgcgtcgctt
     7621 caagcgttcc tgcgaaaaag caggtgttct ggcggaagtt cgtcgtcgtg agttctatga
     7681 aaaaccgact accgaacgta agcgcgctaa agcttctgca gtgaaacgtc acgcgaagaa
     7741 actggctcgc gaaaacgcac gccgcactcg tctgtactaa ttccccgaga gcgttgctct
     7801 ccgatcagac cgagttgtag ttgtaaggcc gtgcttccga aaggaatgcg cggcttattt
     7861 tcgtttatga attgctaaaa atcggggcct atggctggac gaatcccacg cgtattcatt
     7921 aatgatctgc tggcacgcac tgacatcgtc gatctgatcg atgcccgtgt gaagctgaaa
     7981 aagcagggca agaatttcca cgcgtgttgt ccattccaca acgagaaaac cccgtcattc
     8041 accgttaacg gtgagaaaca gttttaccac tgctttggat gtggcgcgca cggcaacgcg
     8101 atcgacttcc tgatgaacta cgacaagctc gagttcgttg aaacggtcga agagctggca
     8161 gcaatgcaca atcttgaagt gccatttgaa gcaggcagcg gccccagcca gatcgagcgc
     8221 catcagaggc aaacgcttta tcagttgatg gacggtctga atacgtttta ccaacaatct
     8281 ttacaacaac ctgttgccac gtctgcgcgc cagtatctgg aaaaacgcgg attaagccac
     8341 gaggttatcg ctcgctttgc gattggtttt gcgccccccg gctgggacaa cgtcctgaag
     8401 cggtttggcg gcaatccaga aaatcgccag tcattgattg atgcggggat gttggtcact
     8461 aacgatcagg gacgcagtta cgatcgtttc cgcgagcggg tgatgttccc cattcgcgat
     8521 aaacgcggtc gggtgattgg ttttggcggg cgcgtgctgg gcaacgatac ccccaaatac
     8581 ctgaactcgc cggaaacaga cattttccat aaaggccgcc agctttacgg tctttatgaa
     8641 gcgcagcagg ataacgctga acccaatcgt ctgcttgtgg tcgaaggcta tatggacgtg
     8701 gtggcgctgg cgcaatacgg cattaattac gccgttgcgt cgttaggtac gtcaaccacc
     8761 gccgatcaca tacaactgtt gttccgcgcg accaacaatg tcatttgctg ttatgacggc
     8821 gaccgtgcag gccgcgatgc cgcatggcga gcgctggaaa cggcgctgcc ttacatgaca
     8881 gacggccgtc agctacgctt tatgtttttg ccggatggcg aagaccctga cacgctggta
     8941 cgaaaagaag gtaaagaagc gtttgaagcg cggatggagc aggcgatgcc actctccgca
     9001 tttctgttta acagcctgat gccgcaagtt gatctgagta cccctgacgg gcgcgcacgt
     9061 ttgagtacgc tggcactgcc attgatatcg caagtgccgg gcgaaacgct gcgaatatat
     9121 cttcgtcagg aattaggcaa caaattaggc atacttgatg acagccagct tgaacgatta
     9181 atgccaaaag cggcagagag cggcgtttct cgccctgttc cgcagctaaa acgcacgacc
     9241 atgcgtatac ttatagggtt gctggtgcaa aatccagaat tagcgacgtt ggtcccgccg
     9301 cttgagaatc tggatgaaaa taagctccct ggacttggct tattcagaga actggtcaac
     9361 acttgtctct cccagccagg tctgaccacc gggcaacttt tagagcacta tcgtggtaca
     9421 aataatgctg ccacccttga aaaactgtcg atgtgggacg atatagcaga taagaatatt
     9481 gctgagcaaa ccttcaccga ctcactcaac catatgtttg attcgctgct tgaactgcgc
     9541 caggaagagt taatcgctcg tgagcgcacg catggtttaa gcaacgaaga acgcctggag
     9601 ctctggacat taaacctgga gctggcgaaa aagtgattta acggcttaag tgccgaagag
     9661 cgatcgggaa gcccccgaca gccgcactga gaggcagcgg caaatatata agtacgccct
     9721 cgtaattatc gttggcggta aacaaccgtt ggatttcagc gttaacggct gaaggacatc
     9781 gggtcaatcg cccaacacca acctcatgaa ataagtgtgg ataccgtctt atggagcaaa
     9841 acccgcagtc acagctgaaa cttcttgtca cccgtggtaa ggagcaaggc tatctgacct
     9901 atgccgaggt caatgaccat ctgccggaag atatcgtcga ttcagatcag atcgaagaca
     9961 tcatccaaat gatcaacgac atgggcattc aggtgatgga agaagcaccg gatgccgatg
    10021 atctgatgct ggctgaaaac accgcggacg aagatgctgc cgaagccgcc gcgcaggtgc
    10081 tttccagcgt ggaatctgaa atcgggcgca cgactgaccc ggtacgcatg tacatgcgtg
    10141 aaatgggcac cgttgaactg ttgacccgcg aaggcgaaat tgacatcgct aagcgtattg
    10201 aagacgggat caaccaggtt caatgctccg ttgctgaata tccggaagcg atcacctatc
    10261 tgctggaaca gtacgatcgt gttgaagcag aagaagcgcg tctgtccgat ctgatcaccg
    10321 gctttgttga cccgaacgca gaagaagatc tggcacctac cgccactcac gtcggttctg
    10381 agctttccca ggaagatctg gacgatgacg aagatgaaga cgaagaagat ggcgatgacg
    10441 acagcgccga tgatgacaac agcatcgacc cggaactggc tcgcgaaaaa tttgcggaac
    10501 tacgcgctca gtacgttgta acgcgtgaca ccatcaaagc gaaaggtcgc agtcacgctg
    10561 ccgctcagga agagatcctg aaactgtctg aagtattcaa acagttccgc ctggtgccga
    10621 agcagtttga ctacctggtc aacagcatgc gcgtcatgat ggaccgcgtt cgtacgcaag
    10681 aacgtctgat catgaagctc tgcgttgagc agtgcaaaat gccgaagaaa aacttcatta
    10741 ccctgtttac cggcaacgaa accagcgata cctggttcaa cgcggcaatt gcgatgaaca
    10801 agccgtggtc ggaaaaactg cacgatgtct ctgaagaagt gcatcgcgcc ctgcagaaac
    10861 tgcagcagat tgaagaagaa accggcctga ccatcgagca ggttaaagat atcaaccgtc
    10921 gtatgtccat cggtgaagcg aaagcccgcc gtgcgaagaa agagatggtt gaagcgaact
    10981 tacgtctggt tatttctatc gccaagaaat acaccaaccg tggcttgcag ttccttgacc
    11041 tgattcagga aggcaacatc ggtctgatga aagcggttga taaattcgaa taccgccgtg
    11101 gttacaagtt ctccacctac gcaacctggt ggatccgtca ggcgatcacc cgttctatcg
    11161 cggatcaggc gcgcaccatc cgtattccgg tgcatatgat tgagactatc aacaaactca
    11221 accgtatttc tcgccagatg ctgcaagaga tgggccgtga accgacgccg gaagaactgg
    11281 ctgaacgtat gctgatgccg gaagacaaga tccgcaaagt gctgaagatc gccaaagagc
    11341 caatctccat ggaaacgccg atcggtgatg atgaagattc gcatctgggg gatttcatcg
    11401 aggataccac cctcgagctg ccgctggatt ctgcgaccac cgaaagcctg cgtgcggcaa
    11461 cgcacgacgt gctggctggc ctgaccgcgc gtgaagcgaa agttctgcgt atgcgtttcg
    11521 gtatcgatat gaacaccgac cacacgctgg aagaagtggg taaacagttc gacgttaccc
    11581 gcgaacgtat ccgtcagatc gaagcgaagg cgctgcgcaa actgcgtcac ccgagccgtt
    11641 ctgaagtgtt gcgtagcttc ctggacgatt aatcggtagg ccggatcagg cgttacgccg
    11701 cacccggcac tagtcccttt gcacaaacgc caccttttcg gtggcgtttt ttatcgccca
    11761 cgcactacca gcgcctggtc cagctcgcga tatgcttcaa ccagtttctc tagtgaaacg
    11821 cgacttaaac cgctgggatt tggcagcacc caaatctgcg tcgaaccaat ggtgagcgtt
    11881 tgtttccccc actgtgcacc gcgctggctg aatccctgtt catatgcttg tttgcccaga
    11941 atcgccaacg cctgcggctg ataatcttca attttttcaa tcagcttacg cccacctgcg
    12001 tgcagctcct gctttgaaac ttcattggct tgcaccgttg gacggtctac cagtttggtg
    12061 acgccacaac gataatccag cagatgctgt gcctcctgcg gcttcaactg acggtcggta
    12121 aacccggcct gatatatcac cttccagaag cgatttgctg gatgagcaaa gggaaaacca
    12181 gtcccggcgg atgaaagccc agggttgata ccgcaaaaca cgacccgtaa ccctggagcc
    12241 aaaatatcct caaccatatt tactcctttc taacatcacc aggaaaagta taacggattg
    12301 aaaatacatt gtttataaaa acagcaggcg cacggtaatg gctggattgc gacacggagt
    12361 tactttataa tccgctacca tggcccctta gctcagtggt tagagcaggc gactcataat
    12421 cgcttggtcg ctggttcaag tccagcaggg gccaccagat atagcaaagg ctgacgagaa
    12481 atcgtcagcc tttttctttt tatatatcag ttactttgcg tgccagtaag ccgctgcacg
    12541 tacccgctgt gggtcatact gttccgcttc aaagcggcgg cttaaattct taacgacttt
    12601 accttcgccg gttatccaga tgaagtaatc atcggcaggg atttgcatct gcgccagacg
    12661 cgcatctacc gcctgctcat catgtgccag ccattcgata ttaaaaccat caaggtgcgc
    12721 gagataatcc tgacaggcgt tatcccgcac gctaaccagc gcactaactt gcggtttaac
    12781 ggcaagtttg ctcaacgttt ccaggcggcg gcgcagtgcg ggcattccgg attcatcgca
    12841 gacatacagc tgatacgcgt aatcttccgg caccaccagc gaaccgcgcg gacctgccac
    12901 cgtaagttta tcgcccggtt gcgcctgcat cgcccagccg ctggcgaccc caccgtcgtg
    12961 aataaagaaa tcgatcgcca gttcatggcg tagttcgtca tacagcggcg tatagtcacg
    13021 cgacagtggg cgtggtcctt ccggccagac gatgccctct tccgttaccg ttggcggcac
    13081 aaagtgagcg tcaggttgag gaaagaagag tttgctgtga tcgtcaaagc cgcgcgatgt
    13141 aaaaccgtcc agcgcctcgc cgccgaggac aatgcgctga aaaccggcgc tgatgcgctc
    13201 aacgcgtaac acagtcagtt cacggaagcg cagatcattg cgaacgcgct gcgggtagcg
    13261 gggggagtta ttcattgtta tcgccttcgt gatagtaatc agatatatct aaatgatttt
    13321 cgtaaatgat aatgattgtt aatcatgata aatgcaagcg atttgtagaa ctgatatatc
    13381 tgaagtatgt gaaggcattc agtgccatag caatgctgcc ggatgcggcg tgaacgtctt
    13441 atccggcctg gatgtgaggt gcttccacgt ttaaaaatca tttttgtact tgcagtaatg
    13501 ctcacatcag atataaatta gatatatcta atcaagcaaa aggaggctga tatgagccat
    13561 catcacgaag ggtgttgtaa acatgaaggc cagccacgcc atgagggctg ctgcaaaggt
    13621 gagaagtcag aacacgagca ctgcggacac ggtcaccagc atgaacacgg tcaatgctgc
    13681 ggtggtcgcc acggtcgcgg cggcggtcgt cggcaacgtt tctttggtca cggtgaattg
    13741 cgtctggtga ttctggatat tctctcgcgc gatgacagcc acggttacga attgattaaa
    13801 gcgattgaga acctaaccca ggggaattac accccaagcc cgggcgtcat ctacccaacg
    13861 ctggattttc tgcaggagca gtcgctgatt accatccgcg aagaggaagg tggtaagaag
    13921 cagattgcgc tgaccgaaca aggcgcgcag tggctggaag aaaaccgcga acaggtggag
    13981 atgattgaag aacgcatcaa agcgcgttgc gttggcgcgg cgctgcgcca gaacccgcaa
    14041 atgaagcggg cgctggataa ttttaaagcg gtgctggatt tacacgtcaa ccagagcgat
    14101 atcagtgatg cacaaataaa aaagatcatt gcggtgatcg accgcgccgc ttttgatatt
    14161 acgcaactgg attaatcgcc gcatccgcca gtggcgcggt gcaattgccg gatgcgacgc
    14221 ttgacgcgcc ttatccggcc tacacccgct acacaccccg caggcctgat aagatgcgcc
    14281 agcatcgcat caggcattgt gctccaaccg ccggatccgg cataccgatt aatgcagcac
    14341 cgtcaccgcg tcttccagtc ggctggcgcg gtgtttcacc atcgccgaca cctgcgcact
    14401 ctcttccacc agctcggcat ttttctgggt gatcaggtta agctcatcca ctgcacgggt
    14461 caggctggaa agcccatcgg cctgttccag cgttgaatgg ctaatctggg cgatcaactg
    14521 ggtgacgttt ttcacctgtg ccacaatatc ttccatcgtc cgtccggcgg cgtgtacctg
    14581 ctgcgaaccg gattgcacct tatcagcact ggcatcaatc agcttgcgaa tatcgttggc
    14641 agcattagcg ctgcggctgg ctaaatgacg cacttcccct gccaccactg caaaaccttt
    14701 gccctgttcg ccggcacgcg ccgcttccac cgccgcattc agggccagaa tattggtctg
    14761 aaacgcaata tcgttaatca gcgaagtaat ggtgccaatg cgctgggtac tgtcggcgat
    14821 atcgtccatt gtcttgatca ccgtggtcat cgcctcccca ccctgcactg ccgcattact
    14881 ggcggtgata gaaagtttat cggcagccga cgccgtggca gagttctgtt tcaccgacgc
    14941 cgccatttgg ttcatggtcg ccaccgtttg ctgaacgtta tcaactgtct gctgggtatg
    15001 ttcgttcagt tcatcggtgc ctttcgccag cgtctcactg ccatttctga cactggacac
    15061 ctggcttgag acatcgttaa ttagccaacg gcacatcagg ccaagctgcc ctaccgcgcg
    15121 taatgtcagc cccagctcat cgctgcgatt caggtgctca acactattac gctctccggt
    15181 cgccaccttc agcgcctgac gggcgacatt ttctatcggg cgcacaatct gccattcaaa
    15241 acaagcgctt gccaacaata ccactaacac acagaggaaa tacgtcacca ccggggcagc
    15301 aacaaaccaa agcatggccg ccagcaagat aaacatcagg gtcatcactc cacgcgcccg
    15361 ccagcgaagc ggtaatgaag gcagtttacc cagccagcct ttacgcacca ccaggccttt
    15421 atgaatacgc ttactggtac gtccggcgtt cagcgctttg tacagcggct ccaccgccgc
    15481 aatctcttca tccgtcgccc gggtacgaat cgacatatag ccactgattt ttccctcgcg
    15541 caccatcggt accgcattgg cccgcaccca ataatggtcg ccatttttgc ggcgattttt
    15601 cacgatgccg ctccagggct cccctttttt cagggtgaac cacatatccg caaacgccgc
    15661 ttttggcata tccgggtgac gcaccatgtt gtgcggctgc ccttgtaact cttgcaaggt
    15721 aaagccgctc acctgcacaa aagtgtcatt agcatgagtg atatagcttt gcagatcggt
    15781 agtggacatc agagtggtat cgtccgccag cggggtattt tgctgggtga catacggatg
    15841 agaagacatg ttcgcgtcct gtgcaggtta tatggttgtt aacttcttgt cagagtttat
    15901 gtcggccccg ctgcggttat ctttaaccga ttaatttgat ttagatcgca atttgcgatt
    15961 aaagcacaaa tctaattctt tgatttaaaa tactttcact ctgttactat acgaaaacgt
    16021 taattatctt gcccaaaaat caggcaatta ttgccctgaa aacgtgcatt tgcgcagcaa
    16081 tcatcaaatc catacccgac aaaaaccgtg caaaataaca acaaatgtta acagatagca
    16141 ttaaatattg cacaaatgat aaccgaattt gtgtttatcc cgattttcgc gatcgcagcc
    16201 ggagtggcgc aatccctgca atacttaaat cggtatcatg tgatacgcga gcctccggag
    16261 catattttga acaggttacc ttcgagcgca tcggctttag cgtgcagcgc ccacgccctg
    16321 aatctcattg agaagcgaac gctggatcat gaggagatga aagcacttaa ccgagaggtg
    16381 attgaatact tcaaagagca tgtcaatccg gggtttttag agtatcgcaa atctgttacc
    16441 gccggcgggg attacggagc cgtagagtgg caagcgggag gtttaaatac gcttgtcgac
    16501 acccagggac aggagtttat cgactgcctg ggaggttttg gaattttcaa cgtggggcac
    16561 cgtaatccag ttgtggtttc cgccgtacag aatcaacttg cgaaacaacc gctgcacagc
    16621 caggagctgc tcgatccgtt acgggcgatg ttggcgaaaa cccttgctgc gctaacgccc
    16681 ggtaaactga aatacagctt cttctgtaat agcggcaccg agtccgttga agcagcgctg
    16741 aagctggcga aagcttacca gtcaccgcgc ggcaagttta cttttattgc caccagcggc
    16801 gcgttccacg gtaaatcact tggcgcgctg tcggccacgg cgaaatcgac cttccgcaaa
    16861 ccgtttatgc cactgctgcc gggcttccgt catgtgccgt ttggcaatat cgaagccatg
    16921 cgcacggctc ttaacgagtg caaaaaaacc ggtgatgatg tggctgcggt gatcctcgaa
    16981 ccgattcagg gtgaaggtgg cgtaattctg ccgccgccgg gctatctcac cgccgtacgt
    17041 aagctatgcg atgagttcgg cgcactgatg atcctcgatg aagtacaaac gggcatgggg
    17101 cgcacgggca agatgttcgc ctgcgagcat gagaacgtac agccggatat cctctgcctt
    17161 gccaaagcgc tcggcggcgg cgtgatgccg attggcgcga ccatcgccac tgaagaggtg
    17221 ttttcagttc tgttcgacaa cccattcctg cataccacca cctttggcgg caacccgctg
    17281 gcctgtgcgg cggcgctggc gaccatcaat gtgttgctgg agcagaactt accggctcag
    17341 gctgagcaaa aaggcgatat gttgctggac ggtttccgtc aactggcgcg ggaatatccc
    17401 gatctggtac aggaagcgcg tggtaaaggg atgttgatgg caattgagtt tgttgataac
    17461 gaaatcggct ataactttgc cagcgagatg ttccgccagc gcgtactggt ggccggaacg
    17521 ctcaataacg ccaaaacgat ccgcattgaa ccgccactga cactgaccat tgaacagtgt
    17581 gaactggtga tcaaagcggc gcgtaaggcg ctggcggcca tgcgagtaag tgtcgaagaa
    17641 gcgtaatatc aaatcggatg gcgatacgac gtcgccatcc gatcttttta tacgacgcgt
    17701 acgcccgcag gcatcatccg ctccggcgtt aacaacacgc tttcactgcc gtcatcggtt
    17761 tccgcacaca acagcatgca ttccgacgtt tcaccacgca ttttcgcttt ttgcagattg
    17821 cataagacca ccaccgtttt ccccatcagc tcttcttcgc tgtagtacgg caccaggctg
    17881 gtcacggttt gcagtgtttt ttcccccaca tcaacctgta cgatgtacag cttgtcggcg
    17941 ttttcatggc gtttcacttc cacaatcttt ccgacgcgca tttccagacg tgcaaaatca
    18001 gcgtaagcca cggtttccat tgtttcctcc cttcgggtaa aattttacta aactatagca
    18061 aaagtttttc tcaatcctgt aggctaaaaa tggagaatgc aggcgtgatc acattcgtga
    18121 gccgctgtgt taccgttaca gcgtcaaaga aacgcgcttt atttactgaa aacaggtgac
    18181 ccgataagca cttcctctac aatgggggcg cacatcaggg aaagtaaaaa aggtaaacat
    18241 ggcaacacta aaagacatcg caatcgaagc tggcgtatcc ctggcgacag tatccagggt
    18301 cttaaatgac gatccgacat tgaatgtgaa agaagagacg aaacatcgca ttctcgagat
    18361 cgccgaaaag ctggagtaca agaccagtag tgcccgtaaa ctccagacag gtgcagtcaa
    18421 ccaacaccat attctggcta tctacagcta ccagcaggag ctggagatca acgatcctta
    18481 ctatctggcg atccgccacg gcattgaaac ccagtgcgaa aagctgggca tcgagctcac
    18541 caactgttat gaacacagcg gcttaccaga cattaaaaac gtcaccggta ttttaattgt
    18601 cggcaaaccc acgcccgccc tgcgcgccgc tgccagcgcg ttgaccgaca atatctgttt
    18661 tatcgacttt cacgaacccg gcagcggtta cgatgcggtg gatatcgatc tggcacgcat
    18721 cagtaaagaa atcatcgact tctatatcaa ccagggcgtt aatcgtattg gttttattgg
    18781 cggtgaagat gagcctggca aggcggatat tcgtgaggtc gcctttgcgg aatatggccg
    18841 actgaaacaa gtggtacgcg aagaggatat ctggcgcggc ggtttttcca gttcgtcggg
    18901 ttatgaactg gcaaaacaaa tgctggcgcg ggaagactat ccgaaggcac tgtttgttgc
    18961 ttccgattcc attgctatcg gcgtactgcg ggcaattcat gaacgaggcc tgaacatccc
    19021 acaggatatt tcgcttatca gcgttaacga tatccccacc gcgcgattta cctttccgcc
    19081 gctctccacc gtgcgcatcc attccgagat gatgggaagt cagggcgtta acctggtgta
    19141 tgaaaaagcc cgcgatggtc gcgcgctgcc gctgttagtc ttcgttccca gcaaattaaa
    19201 actgcgcggc acgacccgtt aaatcccctt acacactgtc cggcaatcgt ttttgccgga
    19261 cagtgctgcc gtttattttc gtgatccagt taaagtaaat gcatttacct gctacttttt
    19321 agtaaaaatt ttactaaact ccccagcaat tacacaaact accatcacca tgaatggttc
    19381 cgatttctct ctaccgggag gccctatgaa tcgctgggaa aacattcagc tcacccacga
    19441 aaaccgactt gcgccgcgtg cgtacttttt ttcatatgat tctgttgcgc aagcgcgtac
    19501 ctttgcccgc gaaaccagca gcctgtttct gcccttaagc ggtcagtgga atttccactt
    19561 ttttgaccat ccgctgcaag taccagaagc cttcacctct gagttaatgg ctgactgggg
    19621 gcatattacc gtccccgcca tgtggcaaat ggaaggtcac ggcaaactgc aatataccga
    19681 cgaaggtttt ccgttcccca tcgatgtgcc gtttgtcccc agcgataacc caaccggtgc
    19741 ctatcaacgt attttcaccc tcagcgacgg ctggcagggt aaacagacgc tgattaaatt
    19801 tgacggcgtc gaaacctatt ttgaagtcta tgttaacggt cagtatgtgg gtttcagcaa
    19861 gggcagtcgc ctgaccgcag agtttgacat cagcgcgatg gttaaaaccg gcgacaacct
    19921 gttgtgtgtg cgcgtgatgc agtgggcgga ctctacctac gtggaagacc aggatatgtg
    19981 gtggtcagcg gggatcttcc gcgatgttta tctggtcgga aaacacctaa cgcatattaa
    20041 cgatttcact gtgcgtaccg actttgacga agcctattgc gatgccacgc tttcctgcga
    20101 agtggtgctg gaaaatctcg ccgcctcccc tgtcgtcacg acgctggaat ataccttgtt
    20161 tgatggcgaa cgcgtggtgc acagcagcgc cattgatcat ttggcaattg aaaaactgac
    20221 cagcgccagc tttgctttta ctgtcgaaca gccgcagcaa tggtcagcag aatcccctta
    20281 tctttaccat ctggtcatga cgctgaaaga cgccaacggc aacgttctgg aagtggtgcc
    20341 acaacgcgtt ggcttccgtg atatcaaagt gcgcgacggt ctgttctgga tcaataaccg
    20401 ttatgtgatg ctgcacggcg tcaaccgtca cgacaacgat catcgcaaag gccgcgccgt
    20461 tggaatggat cgcgtcgaga aagatctcca gttgatgaag cagcacaata tcaactccgt
    20521 gcgtaccgct cactacccga acgatccgcg tttttacgaa ctgtgtgata tctacggctt
    20581 gtttgtgatg gcggaaaccg acgtcgaatc gcacggcttt gctaatgtcg gcgatatcag
    20641 ccgtattacc gacgatccgc agtgggaaaa agtctacgtc gagcgcattg ttcgccatat
    20701 tcacgcgcag aaaaaccatc cgtcgatcat catctggtcg ctgggcaatg aatccggcta
    20761 tggctgtaac atccgcgcga tgtaccacgc agcgaaggcg ctggatgaca cgcgactggt
    20821 gcattacgaa gaagatcgcg atgctgaagt ggtcgatatt atttccacca tgtacacccg
    20881 cgtgccgctg atgaatgagt ttggtgaata cccgcatccg aagccgcgca tcatctgtga
    20941 atatgctcat gcgatgggga acggaccagg cgggctgacg gagtaccaga acgtcttcta
    21001 taagcacgat tgtattcagg gacattatgt ttgggagtgg tgcgaccacg ggatccaggc
    21061 gcaggatgac aacggcaacg tctggtataa attcggcggc gactacggcg actatcccaa
    21121 caactataac ttctgtcttg atggtttgat ctattccgat cagacgccgg gaccgggcct
    21181 gaaagagtac aaacaggtta tcgcgccggt aaaaatccac gcgctggatc tgactcgcgg
    21241 cgagctgaaa gtcgaaaata aactgtggtt taccacgctt gatgactaca ccctgcacgc
    21301 agaggtgcgc gtcgaaggtg aaacgctcgc gacgcagcag attaaactgc gcgacgttgc
    21361 gccgaacagc gaagccccct tgcagatcac gctgccgcag ctggacgccc gcgaagcgtt
    21421 cctcaacatt acggtgacca aagattcccg cacccgctac agcgaagccg ggcattctat
    21481 cgccacttat cagttcccgc tgaaggaaaa caccgcgcaa ccagtgcctt tcgcaccaaa
    21541 taatgcgcgt ccgctgacgc tggaagacga tcgtttgagc tgcaccgttc gcggctacaa
    21601 cttcgcgatc accttctcaa aaatgagtgg caaaccgaca tcctggcagg tgaatggcga
    21661 gtcgctgctg acccgcgagc caaagatcaa cttcttcaag ccaatgatcg acaaccacaa
    21721 gcaggagaac gaagggctgt ggcaaccgaa tcatttgcag atcatgcagg aacatctgcg
    21781 cgactttgcc gtagaacaga gcgatggtga agtgttgatc atcagccgca cagttattgc
    21841 cccgccggtg tttgacttcg ggatgcgctg cacctacatc tggcgcatcg ctgccgatgg
    21901 ccaggttaac gtggcgcttt ccggcgagcg ttacggcgac tatccgcaca tcattccgtg
    21961 catcggtttc accatgggga ttaacggcga atacgatcag gtggcgtatt acggtcgtgg
    22021 accgggcgaa aactacgccg acagccagca ggctaacatc atcgatatct ggcgcagcac
    22081 cgtcgatgcc atgttcgaga actatccctt cccgcagaac aacggcaacc gtcagcatgt
    22141 ccgctggacg gcactgacta accgccacgg caacggtctg ctggtggttc cgcagcgccc
    22201 aattaacttc agcgcctggc actataccca ggaaaacatc cacgctgccc agcactgtaa
    22261 cgagctgcag cgcagtgatg acatcaccct gaacctcgac caccagctac ttggcctcgg
    22321 ctccaactcc tggggcagcg aggtgctgga ctcctggcgc gtctggttcc gtgacttcag
    22381 ctacggcttt acgttgctgc cggtttctgg cggagaagct accgcgcaaa gcctggcgtc
    22441 gtatgagttc ggcgcagggt tcttttccac gaatttgcac agcgagaata agcaatgagg
    22501 atcatcgata acttagaaca gttccgccag atttacgcct ctggcaagaa gtggcaacgc
    22561 tgcgttgaag cgattgaaaa tatcgacaac attcagcctg gcgtcgccca ctccatcggt
    22621 gactcattga cttaccgcgt ggagacagac tccgcgaccg atgcgctatt taccgggcat
    22681 cgacgctatt ttgaagtgca ttactacctg caagggcagc aaaaaattga atatgcaccg
    22741 aaagagacat tacaggtagt ggaatattat cgtgatgaaa ctgaccgtga atatttaaaa
    22801 ggctgcggag aaaccgttga ggtccacgaa gggcaaatcg ttatttgcga tatccatgaa
    22861 gcgtatcggt ttatctgcaa taacgcggtc aaaaaagtgg ttctcaaagt caccatcgaa
    22921 gatggttatt tccataacaa ataacaacta cggcggcaaa aggagtttgc cgccaccgct
    22981 accctacaca ttttcggaga tgtgttatgt ctgataccaa acgtaataca atcggcaaat
    23041 tcggcttgct ctcgctgact tttgccgccg tttacagctt taacaacgtt atcaataata
    23101 atattgagct tggactggcc tcggcaccga tgtttttcct cgcgacgatt ttttatttta
    23161 ttcccttctg tctgatcatc gcagaatttg tttcgttaaa taaaaactca gaagccggtg
    23221 tctacgcgtg ggtaaaaagt tcgctgggcg gacgttgggc atttattact gcctatacct
    23281 actggttcgt aaacctgttc tttttcacct cactgttgcc gcgcgttatt gcttatgctt
    23341 cgtatgcctt cctcggatac gaatatatta tgacgccggt tgccaccacc attatcagta
    23401 tggtgctgtt cgccttctcc acctgggttt ccaccaacgg ggcgaaaatg ttggggccaa
    23461 ttacctccgt cacttcaacg ctgatgctgc tgttaacgct ctcctacatt ttactggcag
    23521 gtacggcgct ggttggcggc gtacagcctg ctgaccccat caccgttgac gcgatgatcc
    23581 cgaacttcaa ctgggcgttc ctcggcgtta ccacctggat ctttatggcc gcaggtggcg
    23641 cggagtccgt cgctgtgtac gttaacgacg tcaaaggcgg ttcgaaatcg ttcgttaaag
    23701 tgatcatcct cgccgggatt tttatcggcg tactgtattc cgtctcctcg gtgctgatta
    23761 acgtcttcgt cagcagcaaa gagttgaaat ttaccgtcgg atcggtgcag gtattccacg
    23821 gcatggcggc gtattttggt ctaccggaag cgttgatgaa tcgctttgtc ggtctggtgt
    23881 cctttaccgc gatgttcggt tccctgctga tgtggaccgc aacgccggtg aaaattttct
    23941 tctccgaaat cccggaaggc atctttggta agaaaaccgt cgaactgaac gaaaacggcg
    24001 ttccggcgcg cgcagcgtgg atccagttcc tgatcgtcat cccgctgatg attatcccga
    24061 tgctcggttc caataccgtg caggatctga tgaatactat tattaatatg accgccgcag
    24121 cgtccatgct tccgccgtta ttcatcatgc tggcttacct gaatttacgc gccaaattag
    24181 atcacctgcc acgcgatttc cgtatgggct cccgccgcac cggtattatc gttgtttcaa
    24241 tgctgattgc gatatttgcc gtagggtttg tcgcttcgac attcccgact ggcgcgaata
    24301 ttctgaccat cattttttat aacgtcggcg gtattgttat cttccttggc tttgcgtggt
    24361 ggaaatacag taaatatata aagggattaa cggctgaaga gcgccatatt gaagcgacgc
    24421 cagccagcaa tgttgattaa gcagaataat aaaaaatgag ca
//
LOCUS       DAZKHF020000048        24410 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2201S31241
            SAMN43267800-rid25106413.denovo.048, whole genome shotgun sequence.
ACCESSION   DAZKHF020000048 DAZKHF020000000
VERSION     DAZKHF020000048.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267800
            Sequence Read Archive: SRR30307263
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 24410)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24410)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 24410)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350581.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350581.2
            Genome Coverage       :: 151x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:21:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,652
            CDSs (total)                      :: 5,557
            Genes (coding)                    :: 5,320
            CDSs (with protein)               :: 5,320
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 237
            CDSs (without protein)            :: 237
            Pseudo Genes (ambiguous residues) :: 0 of 237
            Pseudo Genes (frameshifted)       :: 102 of 237
            Pseudo Genes (incomplete)         :: 130 of 237
            Pseudo Genes (internal stop)      :: 52 of 237
            Pseudo Genes (multiple problems)  :: 41 of 237
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24410
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267800-rid25106413.denovo.048"
                     /serotype="O 26:H11"
                     /isolate="2201S31241"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-01"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            172..1377
                     /gene="gltS"
                     /locus_tag="ACHOSC_RS07760"
                     /old_locus_tag="pgaptmp_001552"
     CDS             172..1377
                     /gene="gltS"
                     /locus_tag="ACHOSC_RS07760"
                     /old_locus_tag="pgaptmp_001552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015501 - glutamate:sodium symporter
                     activity [Evidence IEA];
                     GO_process: GO:0015813 - L-glutamate transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sodium/glutamate symporter"
                     /protein_id="HGU4547518.1"
                     /translation="MFHLDTLATLVAATLTLLLGRKLVHSVSFLKKYTIPEPVAGGLL
                     VALALLVLKKSMGWEVNFDMSLRDPLMLAFFATIGLNANIASLRAGGRVVGIFLIVVV
                     GLLVMQNAIGIGMASLLGLDPLMGLLAGSITLSGGHGTGAAWSKLFIERYGFTNATEV
                     AMACATFGLVLGGLIGGPVARYLVKHSTTPNGIPDDQEVPTAFEKPDVGRMITSLVLI
                     ETIALIAICLTVGKIVAQLLAGTAFELPTFVCVLFVGVILSNGLSMMGFYRVFERAVS
                     VLGNVSLSLFLAMALMGLKLWELASLALPMLAILVVQTIFMALYAIFVTWRMMGKNYD
                     AAVLAAGHCGFGLGATPTAIANMQAITERFGPSHMAFLVVPMVGAFFIDIVNALVIKL
                     YLMLPIFAG"
     gene            1380..2246
                     /locus_tag="ACHOSC_RS07765"
                     /old_locus_tag="pgaptmp_001553"
     CDS             1380..2246
                     /locus_tag="ACHOSC_RS07765"
                     /old_locus_tag="pgaptmp_001553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312555.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4547519.1"
                     /translation="MKKRRKEPETLREHCRHIFGDEPPVLCVWETEFDYADAELKALA
                     AKEWQQISEWDLSAYYVLNLVYNEPMQIELFRYLFPLCLAQWHETVLAGGYGDHFEES
                     LMKALCRPYLWQEMMNASQRQQVRQFLLDTALQRMDNERGFNNVLCWLAVFNTLGGAA
                     PLIRSLWSRWWALDTPGKAVCAIQYAAHLIYPIEANPLWSQEWIGWGHPLGHKDGWSS
                     DNRAFLRQMLTPEMIVAGVQAAAEILRGEPEGAMAARIAQDAYEAMDILTIQIEDLLR
                     DLSCDESGHALE"
     gene            complement(2231..4312)
                     /gene="recG"
                     /locus_tag="ACHOSC_RS07770"
                     /old_locus_tag="pgaptmp_001554"
     CDS             complement(2231..4312)
                     /gene="recG"
                     /locus_tag="ACHOSC_RS07770"
                     /old_locus_tag="pgaptmp_001554"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecG"
                     /protein_id="HGU4547520.1"
                     /translation="MKGRLLDAVPLSSLTGVGAALSNKLAKINLHTVQDLLLHLPLRY
                     EDRTHLYPIGELLPGVYATVEGEVLNCNISFGGRRMMTCQISDGSGILTMRFFNFSAA
                     MKNSLAAGRRVLAYGEAKRGKYGAEMIHPEYRVQGDLSTPELQETLTPVYPTTEGVKQ
                     ATLRKLTDQALDLLDTCAIEELLPPELSQGMMTLPEALRTLHRPPPTLQLSDLETGQH
                     PAQRRLILEELLAHNLSMLALRAGAQRFHAQPLSANDTLKNKLLAALPFKPTGAQARV
                     VAEIERDMALDVPMMRLVQGDVGSGKTLVAALAALRAIAHGKQVALMAPTELLAEQHA
                     NNFRNWFAPLGIEVGWLAGKQKGKARLAQQEAIASGQVQMIVGTHAIFQEQVQFNGLA
                     LVIIDEQHRFGVHQRLALWEKGQQQGFHPHQLIMTATPIPRTLAMTAYADLDTSVIDE
                     LPPGRTPVTTVAIPDTRRTDIIDRVHHACITEGRQAYWVCTLIEESELLEAQAAEATW
                     EELKLALPELNVGLVHGRMKPAEKQAVMASFKQGELHLLVATTVIEVGVDVPNASLMI
                     IENPERLGLAQLHQLRGRVGRGAVASHCVLLYKTPLSKTAQIRLQVLRDSNDGFVIAQ
                     KDLEIRGPGELLGTRQTGNAEFKVADLLRDQAMIPEVQRLARHIHERYPQQAKALIER
                     WMPETERYSNA"
     gene            complement(4318..5007)
                     /gene="trmH"
                     /locus_tag="ACHOSC_RS07775"
                     /old_locus_tag="pgaptmp_001555"
     CDS             complement(4318..5007)
                     /gene="trmH"
                     /locus_tag="ACHOSC_RS07775"
                     /old_locus_tag="pgaptmp_001555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0141100 - tRNA
                     (guanine(18)-2'-O)-methyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0030488 - tRNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(18)-2'-O)-methyltransferase
                     TrmH"
                     /protein_id="HGU4547521.1"
                     /translation="MNPTRYARICEMLARRQPDLTVCMEQVHKPHNVSAIIRTADAVG
                     VHEVHAVWPGSRMRTMASAAAGSNSWVQVKTHRTIGDAVAHLKGQGMQILATHLSDNA
                     VDFREIDYTRPTCILMGQEKTGITQEALALADQDIIIPMIGMVQSLNVSVASALILYE
                     AQRQRQNAGMYLRENSMLPEAEQQRLLFEGGYPVLAKVAKRKGLPYPHVNQQGEIEAD
                     ADWWATMQAAG"
     gene            complement(5014..7122)
                     /gene="spoT"
                     /locus_tag="ACHOSC_RS07780"
                     /old_locus_tag="pgaptmp_001556"
     CDS             complement(5014..7122)
                     /gene="spoT"
                     /locus_tag="ACHOSC_RS07780"
                     /old_locus_tag="pgaptmp_001556"
                     /EC_number="2.7.6.5"
                     /EC_number="3.1.7.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008728 - GTP diphosphokinase activity
                     [Evidence IEA];
                     GO_function: GO:0008893 - guanosine-3',5'-bis(diphosphate)
                     3'-diphosphatase activity [Evidence IEA];
                     GO_process: GO:0015969 - guanosine tetraphosphate
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional GTP
                     diphosphokinase/guanosine-3',5'-bis pyrophosphate
                     3'-pyrophosphohydrolase"
                     /protein_id="HGU4547522.1"
                     /translation="MYLFESLNQLIQTYLPEDQIKRLRQAYLVARDAHEGQTRSSGEP
                     YITHPVAVACILAEMKLDYETLMAALLHDVIEDTPATYQDMEQLFGKSVAELVEGVSK
                     LDKLKFRDKKEAQAENFRKMIMAMVQDIRVILIKLADRTHNMRTLGSLRPDKRRRIAR
                     ETLEIYSPLAHRLGIHHIKTELEELGFEALYPNRYRVIKEVVKAARGNRKEMIQKILS
                     EIEGRLQEAGIPCRVSGREKHLYSIYCKMVLKEQRFHSIMDIYAFRVIVNDSDTCYRV
                     LGQMHSLYKPRPGRVKDYIAIPKANGYQSLHTSMIGPHGVPVEVQIRTEDMDQMAEMG
                     VAAHWAYKEHGETSTTAQIRAQRWMQSLLELQQSAGSSFEFIESVKSDLFPDEIYVFT
                     PEGRIVELPAGATPVDFAYAVHTDIGHACVGARVDRQPYPLSQPLTSGQTVEIITAPG
                     ARPNAAWLNFVVSSKARAKIRQLLKNLKRDDSVSLGRRLLNHALGGSRKLNEIPQENI
                     QRELDRMKLATLDDLLAEIGLGNAMSVVVAKNLQHGDASIPPATQSHGHLPIKGADGV
                     LITFAKCCRPIPGDPIIAHVSPGKGLVIHHESCRNIRGYQKEPEKFMAVEWDKETAQE
                     FITEIKVEMFNHQGALANLTAAINTTTSNIQSLNTEEKDGRVYSAFIRLTARDRVHLA
                     NIMRKIRVMPDVIKVTRNRN"
     gene            complement(7141..7416)
                     /gene="rpoZ"
                     /locus_tag="ACHOSC_RS07785"
                     /old_locus_tag="pgaptmp_001557"
     CDS             complement(7141..7416)
                     /gene="rpoZ"
                     /locus_tag="ACHOSC_RS07785"
                     /old_locus_tag="pgaptmp_001557"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013511051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit omega"
                     /protein_id="HGU4547523.1"
                     /translation="MARVTVQDAVEKIGNRFDLVLVAARRARQMQVGGKDPLVPEEND
                     KTTVIALREIEEGLINNQILDVRERQEQQEQEAAELQAVTAIAEGRR"
     gene            complement(7471..8094)
                     /gene="gmk"
                     /locus_tag="ACHOSC_RS07790"
                     /old_locus_tag="pgaptmp_001558"
     CDS             complement(7471..8094)
                     /gene="gmk"
                     /locus_tag="ACHOSC_RS07790"
                     /old_locus_tag="pgaptmp_001558"
                     /EC_number="2.7.4.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000046969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004385 - guanylate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006163 - purine nucleotide metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="guanylate kinase"
                     /protein_id="HGU4547524.1"
                     /translation="MAQGTLYIVSAPSGAGKSSLIQALLKTQPLYDTQVSVSHTTRQP
                     RPGEVHGEHYFFVNHDEFKEMISRDAFLEHAEVFGNYYGTSREAIEQVLATGVDVFLD
                     IDWQGAQQIRQKMPHARSIFILPPSKIELDRRLRGRGQDSEEVIAKRMAQAVAEMSHY
                     AEYDYLIVNDDFDTALTDLKTIIRAERLRMSRQKQRHDALISKLLAD"
     gene            8352..10034
                     /gene="ligB"
                     /locus_tag="ACHOSC_RS07795"
                     /old_locus_tag="pgaptmp_001559"
     CDS             8352..10034
                     /gene="ligB"
                     /locus_tag="ACHOSC_RS07795"
                     /old_locus_tag="pgaptmp_001559"
                     /EC_number="6.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312549.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003911 - DNA ligase (NAD+) activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent DNA ligase LigB"
                     /protein_id="HGU4547525.1"
                     /translation="MKVWMAILISILCWQSSVWAVCPAWSPARAQEEISRLQQQIKQW
                     DDDYWKEGKSEVEDGVYDQLSARLTQWQRCFVSEPRDVMMPPLNGAVMHPVAHTGVRK
                     MADKNALSLWMRERSDLWVQPKVDGVAVTLVYRDGKLNKAISRGNGLKGEDWTQKVSL
                     ISAVPQTVSGPLANSTLQGEIFLQREGHIQQQMGGINARAKVAGLMMRQDDSDTLNSL
                     GVFVWAWPDGPQLMTDRLKELATAGFTLTQRYTRAVKNADEVARVRNEWWKAKLPFVT
                     DGVVVRGAKEPESRHWLPGQAEWLVAWKYQPVAQVAEVKAIQFAVGKSGKISVVASLA
                     PVMLDDKKVQRVNIGSVRRWQEWDIAPGDQILVSLAGQGIPRIDDVVWRGAERTKPTP
                     PENRFNSLTCYFASDVCQEQFISRLVWLGSKQVLGLDGIGEAGWRALHQTHRFEHIFS
                     WLLLTPEQLQNTPGIAKSKSAQLWHRFNLARKQPFTRWVMAMGIPLTRAALNASDERS
                     WSQLLFSTEQFWQQLPGTGSGRARQVIEWKENAQIKKLGSWLAAQQITGFEP"
     gene            complement(10031..10648)
                     /locus_tag="ACHOSC_RS07800"
                     /old_locus_tag="pgaptmp_001560"
     CDS             complement(10031..10648)
                     /locus_tag="ACHOSC_RS07800"
                     /old_locus_tag="pgaptmp_001560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312548.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trimeric intracellular cation channel family
                     protein"
                     /protein_id="HGU4547526.1"
                     /translation="MLLHILYLVGITAEAMTGALAAGRRRMDTFGVIIIATATAIGGG
                     SVRDILLGHYPLGWVKHPEYVIIVATAAVLTTIVAPVMPYLRKVFLVLDALGLVVFSI
                     IGAQVALDMGHGPIIAVVAAVTTGVFGGVLRDMFCKRIPLVFQKELYAGVSFASAVLY
                     IALQHYVSNHDVVIISTLVFGFFARLLALRLKLGLPVFYYSHEGH"
     gene            complement(10940..11764)
                     /gene="dinD"
                     /locus_tag="ACHOSC_RS07805"
                     /old_locus_tag="pgaptmp_001561"
     CDS             complement(10940..11764)
                     /gene="dinD"
                     /locus_tag="ACHOSC_RS07805"
                     /old_locus_tag="pgaptmp_001561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312547.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA damage-inducible protein D"
                     /protein_id="HGU4547527.1"
                     /translation="MNEHHQPFEEIRHYGTEGQEFWSARELAPLLDYRDWRNFQKVLA
                     RATQACEASNQAASDHFVETTKMVVLGSGAQRELEDVHLSRYACYLVVQNGDPAKPVI
                     AAGQTYFAIQTRRQELADDEAFRQLREDEKRLFLRNELKEHNKQLVEAAQQAGVATAI
                     DFAIFQNHGYRGLYGGLDQKAIHQRKGLKKSQKILDHMGSTELAANLFRATQTEEKLK
                     RDGVNSKQQANTTHFDVGSKVRQTIQELGGTMPEELPTPQVSIKQLENSVKITEKK"
     gene            complement(11985..12848)
                     /locus_tag="ACHOSC_RS07810"
                     /old_locus_tag="pgaptmp_001562"
     CDS             complement(11985..12848)
                     /locus_tag="ACHOSC_RS07810"
                     /old_locus_tag="pgaptmp_001562"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005122606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YicC/YloC family endoribonuclease"
                     /protein_id="HGU4547528.1"
                     /translation="MIRSMTAYARREIKGEWGSATWEMRSVNQRYLETYFRLPEQFRS
                     LEPVVRERIRSRLTRGKVECTLRYEPDVSAQGELILNEKLAKQLVTAANWVKMQSDEG
                     EINPVDILRWPGVMAAQEQDLDAIAAEILAALDGTLDDFIVARETEGQALKALIEQRL
                     EGVTAEVVKVRAHMPEILQWQRERLVAKLEDAQVQLENNRLEQELVLLAQRIDVAEEL
                     DRLEAHVKETYNILKKKEAVGRRLDFMMQEFNRESNTLASKSINAEVTNSAIELKVLI
                     EQMREQIQNIE"
     gene            12975..13691
                     /gene="rph"
                     /locus_tag="ACHOSC_RS07815"
                     /old_locus_tag="pgaptmp_001563"
     CDS             12975..13691
                     /gene="rph"
                     /locus_tag="ACHOSC_RS07815"
                     /old_locus_tag="pgaptmp_001563"
                     /EC_number="2.7.7.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006803115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000049 - tRNA binding [Evidence IEA];
                     GO_function: GO:0009022 - tRNA nucleotidyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease PH"
                     /protein_id="HGU4547529.1"
                     /translation="MRPAGRSNNQVRPVTLTRNYTKHAEGSVLVEFGDTKVLCTASIE
                     EGVPRFLKGQGQGWITAEYGMLPRSTHTRNAREAAKGKQGGRTMEIQRLIARALRAAV
                     DLKALGEFTITLDCDVLQADGGTRTASITGACVALADALQKLVENGKLKTNPMKGMVA
                     AVSVGIVNGEAICDLEYVEDSAAETDMNVVMTEDGRIIEVQGTAEGEPFTHEELLTLL
                     ALARGGIESIVATQKAALAN"
     gene            13757..14398
                     /gene="pyrE"
                     /locus_tag="ACHOSC_RS07820"
                     /old_locus_tag="pgaptmp_001564"
     CDS             13757..14398
                     /gene="pyrE"
                     /locus_tag="ACHOSC_RS07820"
                     /old_locus_tag="pgaptmp_001564"
                     /EC_number="2.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004588 - orotate
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009220 - pyrimidine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="orotate phosphoribosyltransferase"
                     /protein_id="HGU4547530.1"
                     /translation="MKPYQRQFIEFALGKQVLKFGEFTLKSGRKSPYFFNAGLFNTGR
                     DLALLGRFYAEALVDSGLEFDLLFGPAYKGIPIATTTAVALAEHHDLDLPYCFNRKEA
                     KDHGEGGNLVGSALQGRVMLVDDVITAGTAIRESMEIIQANGATLAGVLISLDRQERG
                     RGEISAIQEVERDYNCKVISIITLKDLIAYLEEKPEMAEHLAAVKAYREEFGV"
     gene            complement(14435..15031)
                     /gene="slmA"
                     /locus_tag="ACHOSC_RS07825"
                     /old_locus_tag="pgaptmp_001565"
     CDS             complement(14435..15031)
                     /gene="slmA"
                     /locus_tag="ACHOSC_RS07825"
                     /old_locus_tag="pgaptmp_001565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000818604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0010974 - negative regulation of division
                     septum assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoid occlusion factor SlmA"
                     /protein_id="HGU4547531.1"
                     /translation="MAEKQTAKRNRREEILQSLALMLESSDGSQRITTAKLAASVGVS
                     EAALYRHFPSKTRMFDSLIEFIEDSLITRINLILKDEKDTTARLRLIVLLLLGFGERN
                     PGLTRILTGHALMFEQDRLQGRINQLFERIEAQLRQVLREKRMREGEGYTTDETLLAS
                     QILAFCEGMLSRFVRSEFKYRPTDDFDARWPLIAAQLQ"
     gene            complement(15138..15596)
                     /gene="dut"
                     /locus_tag="ACHOSC_RS07830"
                     /old_locus_tag="pgaptmp_001566"
     CDS             complement(15138..15596)
                     /gene="dut"
                     /locus_tag="ACHOSC_RS07830"
                     /old_locus_tag="pgaptmp_001566"
                     /EC_number="3.6.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000976070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004170 - dUTP diphosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006226 - dUMP biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0046081 - dUTP catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dUTP diphosphatase"
                     /protein_id="HGU4547532.1"
                     /translation="MMKKIDVKILDPRVGKEFPLPTYATSGSAGLDLRACLDDAVELA
                     PGDTTLVPTGLAIHIADPSLAAMMLPRSGLGHKHGIVLGNLVGLIDSDYQGQLMISVW
                     NRGQDSFTIQPGERIAQMIFVPVVQAEFNLVEDFDATDRGEGGFGHSGRQ"
     gene            complement(15574..16794)
                     /gene="coaBC"
                     /locus_tag="ACHOSC_RS07835"
                     /old_locus_tag="pgaptmp_001567"
     CDS             complement(15574..16794)
                     /gene="coaBC"
                     /locus_tag="ACHOSC_RS07835"
                     /old_locus_tag="pgaptmp_001567"
                     /EC_number="4.1.1.36"
                     /EC_number="6.3.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418096.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004632 - phosphopantothenate--cysteine
                     ligase activity [Evidence IEA];
                     GO_function: GO:0004633 - phosphopantothenoylcysteine
                     decarboxylase activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0015941 - pantothenate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional phosphopantothenoylcysteine
                     decarboxylase/phosphopantothenate--cysteine ligase CoaBC"
                     /protein_id="HGU4547533.1"
                     /translation="MSLAGKKIVLGVSGGIAAYKTPELVRRLRDRGADVRVAMTEAAK
                     AFITPLSLQAVSGYPVSDSLLDPAAEAAMGHIELGKWADLVILAPATADLIARVAAGM
                     ANDLVSTICLATPAPVAVLPAMNQQMYRAAATQHNLEVLASRGLLIWGPDSGSQACGD
                     IGPGRMLDPLTIVDMAVAHFSPVNDLKHLNIMITAGPTREPLDPVRYISNHSSGKMGF
                     AIAAAAARRGANVTLVSGPVSLPTPPFVKRVDVMTALEMEAAVNASVQQQNIFIGCAA
                     VADYRAATVAPEKIKKQATQGDELTIKMVKNPDIVAGVAALKDHRPYVVGFAAETNNV
                     EEYARQKRIRKNLDLICANDVSQPTQGFNSDNNALHLFWQDGDKVLPLERKELLGQLL
                     LDEIVTRYDEKNRR"
     gene            16966..17634
                     /gene="radC"
                     /locus_tag="ACHOSC_RS07840"
                     /old_locus_tag="pgaptmp_001568"
     CDS             16966..17634
                     /gene="radC"
                     /locus_tag="ACHOSC_RS07840"
                     /old_locus_tag="pgaptmp_001568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709417.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadC"
                     /protein_id="HGU4547534.1"
                     /translation="MKNNAQLLMPREKMLKFGISALTDVELLALFLRTGTRGKDVLTL
                     AKEMLENFGSLYGLLTSEYEQFSGVHGIGVAKFAQLKGIAELARRYYNVRMREESPLL
                     SPEMTREFLQSQLTGEEREIFMVIFLDSQHRVITHSRLFSGTLNHVEVHPREIIREAI
                     KINASALILAHNHPSGCAEPSKADKLITERIIKSCQFMDLRVLDHIVIGRGEYVSFAE
                     RGWI"
     gene            17851..18087
                     /gene="rpmB"
                     /locus_tag="ACHOSC_RS07845"
                     /old_locus_tag="pgaptmp_001569"
     CDS             17851..18087
                     /gene="rpmB"
                     /locus_tag="ACHOSC_RS07845"
                     /old_locus_tag="pgaptmp_001569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008457442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L28"
                     /protein_id="HGU4547535.1"
                     /translation="MSRVCQVTGKRPVTGNNRSHALNATKRRFLPNLHSHRFWVESEK
                     RFVTLRVSAKGMRVIDKKGIDTVLAELRARGEKY"
     gene            18108..18275
                     /gene="rpmG"
                     /locus_tag="ACHOSC_RS07850"
                     /old_locus_tag="pgaptmp_001570"
     CDS             18108..18275
                     /gene="rpmG"
                     /locus_tag="ACHOSC_RS07850"
                     /old_locus_tag="pgaptmp_001570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010847629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HGU4547536.1"
                     /translation="MAKGIREKIKLVSSAGTGHFYTTTKNKRTKPEKLELKKFDPVVR
                     QHVIYKEAKIK"
     gene            18373..19182
                     /gene="mutM"
                     /locus_tag="ACHOSC_RS07855"
                     /old_locus_tag="pgaptmp_001571"
     CDS             18373..19182
                     /gene="mutM"
                     /locus_tag="ACHOSC_RS07855"
                     /old_locus_tag="pgaptmp_001571"
                     /EC_number="3.2.2.23"
                     /EC_number="4.2.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003684 - damaged DNA binding [Evidence
                     IEA];
                     GO_function: GO:0003906 - DNA-(apurinic or apyrimidinic
                     site) endonuclease activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0008534 - oxidized purine nucleobase
                     lesion DNA N-glycosylase activity [Evidence IEA];
                     GO_function: GO:0016799 - hydrolase activity, hydrolyzing
                     N-glycosyl compounds [Evidence IEA];
                     GO_function: GO:0019104 - DNA N-glycosylase activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional DNA-formamidopyrimidine
                     glycosylase/DNA-(apurinic or apyrimidinic site) lyase"
                     /protein_id="HGU4547537.1"
                     /translation="MPELPEVETSRRGIEPHLVGATILHAVVRNGRLRWPVSEEIYRL
                     SDQPVLSVQRRAKYLLLELPEGWIIIHLGMSGSLRILPEELPPEKHDHVDLVMSNGKV
                     LRYTDPRRFGAWLWTKELEGHNVLAHLGPEPLSDDFNGEYLHQKYAKKKTAIKPWLMD
                     NKLVVGVGNIYASESLFAAGIHPDRLASSLSLAECELLARVIKAVLLRSIEQGGTTLK
                     DFLQSDGKPGYFAQELQVYGRKGEPCRVCGTPIVATKHAQRATFYCRQCQK"
     gene            complement(19221..19700)
                     /gene="coaD"
                     /locus_tag="ACHOSC_RS07860"
                     /old_locus_tag="pgaptmp_001572"
     CDS             complement(19221..19700)
                     /gene="coaD"
                     /locus_tag="ACHOSC_RS07860"
                     /old_locus_tag="pgaptmp_001572"
                     /EC_number="2.7.7.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004595 - pantetheine-phosphate
                     adenylyltransferase activity [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pantetheine-phosphate adenylyltransferase"
                     /protein_id="HGU4547538.1"
                     /translation="MQKRAIYPGTFDPITNGHIDIVTRATQMFDHVILAIAASPSKKP
                     MFTLEERVALAQQATAHLGNVEVVGFSDLMANFARNQHATVLIRGLRAVADFEYEMQL
                     AHMNRHLMPELESVFLMPSKEWSFISSSLVKEVARHQGDVTHFLPENVHQALMAKLA"
     gene            complement(19708..20985)
                     /gene="waaA"
                     /locus_tag="ACHOSC_RS07865"
                     /old_locus_tag="pgaptmp_001573"
     CDS             complement(19708..20985)
                     /gene="waaA"
                     /locus_tag="ACHOSC_RS07865"
                     /old_locus_tag="pgaptmp_001573"
                     /EC_number="2.4.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipid IV(A) 3-deoxy-D-manno-octulosonic acid
                     transferase"
                     /protein_id="HGU4547539.1"
                     /translation="MLELLYTALLYLIQPLIWIRLWVRGRKAPAYRKRWGERYGFYRH
                     PLKPGGIMLHSVSVGETLAAIPLVRALRHRYPDLPITVTTMTPTGSERVQSAFGKDVQ
                     HVYLPYDLPDALNRFLNKVDPKLVLIMETELWPNLIAALHKRKIPLVIANARLSARSA
                     AGYAKLGKFVRRLLRRITLIAAQNEEDGARFVALGAKNNQVTVTGSLKFDISVTPQLA
                     AKAVTLRRQWAPHRPVWIATSTHEGEESVVIAAHQALLQQFPNLLLILVPRHPERFPD
                     AINLVRQAGLSYITRSSGEVPSTSTQVVVGDTMGELMLLYGIADLAFVGGSLVERGGH
                     NPLEAAAHAIPVLMGPHTFNFKDICARLEQASGLITVTDATTLAKEVSSLLTDADYRS
                     FYGRHAVEVLYQNQGALQRLLQLLEPYLPPKTH"
     gene            21398..22456
                     /gene="rfaQ"
                     /locus_tag="ACHOSC_RS07870"
                     /old_locus_tag="pgaptmp_001574"
     CDS             21398..22456
                     /gene="rfaQ"
                     /locus_tag="ACHOSC_RS07870"
                     /old_locus_tag="pgaptmp_001574"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide core heptosyltransferase
                     RfaQ"
                     /protein_id="HGU4547540.1"
                     /translation="MDKPFRRILLIKMRFHGDMLLTTPVISSLKKNYPDAKIDVLLYQ
                     DTIPILSENPEINALYGIKNKKAKASEKIANFFHLIKVLRANKYDLIVNLTDQWMVAI
                     LVRLLNARVKISQDYHHRQSAFWRKSFTHLVPLQGGNVVESNLSVLTPLGVDSLVKQT
                     TMSYPPASWKRMRRELDHAGVGQNYVVIQPTARQIFKCWDNAKFSAVIDALHARGYEV
                     VLTSGPDKDDLACVNEIAQGCQTPPVTALAGKVTFPELGALIDHAQLFIGVDSAPAHI
                     AAAVNTPLISLFGATDHIFWRPWSNNMIQFWAGDYREMPTRDQRDRNEMYLSVIPAAD
                     VIAAVDKLLPSSTTGTSL"
     gene            22453..23577
                     /locus_tag="ACHOSC_RS07875"
                     /old_locus_tag="pgaptmp_001575"
     CDS             22453..23577
                     /locus_tag="ACHOSC_RS07875"
                     /old_locus_tag="pgaptmp_001575"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HGU4547541.1"
                     /translation="MIVAFCLYKYFPFGGLQRDFMRIAQTVAARGHHVRVYTQSWEGE
                     CPDVFELIKVPVKSHTNHGRNAEYFAWVQKHLREHPVDKVVGFNKMPGLDVYYAADVC
                     YAEKVAQEKGFFYRLTSRYRHYAAFERATFEQGKPTQLLMLTDKQIADFQKHYQTEAE
                     RFHILPPGIYPDRKYSQQPANSREIFRKKNGITEQQYLLLQVGSDFTRKGVDRSIEAL
                     ASLPDSLRHNTLLYVVGQDKPRKFEALAEKRGVCSNVHFFSGRNDVSELMAAADLLLH
                     PAYQEAAGIVLLEAITAGLPVLTTAVCGYAPYIVEANCGEAIAEPFRQETLNEILRKA
                     LTQSSLRQAWAENARYYADTQDLYSLPEKAADIITGGLDG"
     gene            23570..24367
                     /gene="rfaP"
                     /locus_tag="ACHOSC_RS07880"
                     /old_locus_tag="pgaptmp_001576"
     CDS             23570..24367
                     /gene="rfaP"
                     /locus_tag="ACHOSC_RS07880"
                     /old_locus_tag="pgaptmp_001576"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312532.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide core heptose(I) kinase RfaP"
                     /protein_id="HGU4547542.1"
                     /translation="MVELKEPFATLWRGKDPFEEVKTLQGEVFRELETRRTLRFEMAG
                     KSYFLKWHRGTTLKEIIKNLLSLRMPVLGADREWNAIHRLRDVGVDTMHGVAFGEKGM
                     NPLTRTSFIITEDLTPTISLEDYCADWATNPPDVRVKRMLIKRVATMVRDMHAAGINH
                     RDCYICHFLLHLPFSGKEEELKISVIDLHRAQLRTRVPRRWRDKDLIGLYFSSMNIGL
                     TQRDIWRFMKVYFAAPLKDILKQEQGLLSQAEAKATKIRERTIRKSL"
BASE COUNT         5713 a         6038 c         6586 g         6073 t
ORIGIN      
        1 ttatataact tttggtattt ttctgcttaa aatccatgcc atttgttcat taacagctga
       61 gaatttgacc caggaaagca taactcgata cccgtcatag ttcatgaagt tgccactgcg
      121 cgatgaagta tgacgagtat gaaagagtga tgcggataca aaggagtaac tatgtttcat
      181 ctcgatactt tagcaacgct tgttgccgca acgctgacgt tgctgctcgg gcgtaagttg
      241 gtccattccg tctccttttt gaagaaatac accataccgg aacctgttgc gggtggtttg
      301 ttggtggcgc tggcgctact agtactgaaa aaaagcatgg gctgggaagt caactttgat
      361 atgtccctgc gcgatccgtt aatgctggct ttcttcgcca ccattggcct gaacgccaac
      421 attgccagtt tgcgtgccgg tgggcgtgtg gttggcatct tcttgattgt ggttgttggt
      481 ctgttggtga tgcaaaatgc cattggcatt ggtatggcta gcctgttagg gcttgatccg
      541 ctgatggggc tgttggccgg ttctattacg ctttccggcg gtcacggtac gggcgctgcg
      601 tggagtaaat tgttcattga acgttatggc ttcaccaatg cgacggaagt ggcgatggcc
      661 tgtgcaacgt tcggtctggt gctgggcggc ttgattggcg gtccggtggc gcgctatctg
      721 gtgaaacact ccaccacgcc gaacggtatt ccggatgacc aggaagtccc gacggcgttt
      781 gaaaagccgg atgtgggacg catgatcacc tcgctggtgc tgattgaaac tatcgcgctg
      841 attgctatct gcctgacggt ggggaaaatt gttgcgcaac ttttggctgg cactgctttt
      901 gaactgccga ccttcgtctg tgtactgttt gttggcgtga ttctgagcaa cggtctgtca
      961 atgatgggct tttaccgcgt ctttgagcgt gcggtatccg tgctgggtaa cgtaagcctg
     1021 tcgttgttcc tggcgatggc gctgatgggg ctgaaactgt gggagctggc ttcgctggcg
     1081 ctgccgatgc tggcgattct ggtggtacag accatcttca tggcgttgta tgccatcttc
     1141 gttacctggc gcatgatggg caaaaactac gatgcggcag tgctggctgc gggtcactgt
     1201 ggttttggcc tcggtgcaac gccaacggca atcgccaaca tgcaggcgat cactgaacgc
     1261 tttggcccgt cgcacatggc gtttttggtg gtgccgatgg tcggtgcgtt ctttatcgat
     1321 atcgtcaatg cgctggtgat taagctgtat ttaatgttgc cgatttttgc cggttaaaaa
     1381 tgaaaaagcg tcggaaggaa ccggaaacgc tacgtgaaca ctgtcgccat atttttggtg
     1441 atgagccgcc agtgctctgt gtatgggaaa cggaatttga ttacgcagat gccgaactca
     1501 aggcgctggc ggctaaagag tggcagcaga tcagcgaatg ggatttgagt gcttactatg
     1561 tactcaatct cgtttataac gaaccgatgc aaatagagtt gtttcgctat ctgtttccgc
     1621 tctgcctggc gcaatggcat gaaaccgttt tagctggcgg ttatggtgat cacttcgaag
     1681 aatccttaat gaaggcattg tgtcggccct atctctggca ggagatgatg aatgcttccc
     1741 aacgccagca ggtacggcaa tttttactgg acaccgcgtt gcagcgtatg gataacgagc
     1801 gtggtttcaa taacgttctt tgctggctgg cggtgttcaa tactctgggt ggggccgcgc
     1861 cgttaattcg ttctctctgg tcgcgctggt gggcgctgga tacgccagga aaagcggtgt
     1921 gtgctattca gtatgccgcg catctaattt atcccataga agccaatccg ttatggtcgc
     1981 aagaatggat aggctggggg catccattag gccataaaga tggctggtcc tctgataatc
     2041 gcgcttttct tcgccaaatg ttaacacctg aaatgattgt ggctggcgta caggcagcgg
     2101 ctgaaattct gcgtggtgaa ccggaaggcg caatggcggc gcgaattgca caggatgcgt
     2161 atgaggcaat ggatatcctc actatccaga ttgaagattt actgcgtgac ttgtcttgtg
     2221 atgagtctgg tcatgcgttg gagtaacgtt ccgtctccgg catccagcgt tctatcaggg
     2281 cttttgcttg ttgtgggtaa cgttcgtgaa tatgtcgcgc caggcgctga acttccggga
     2341 tcatcgcctg atcgcgcagt aaatccgcca ctttaaactc agcattaccc gtctgacgcg
     2401 tacctaacag ttcacccggt ccgcgaatct ctaagtcttt ctgtgcaatc acaaaaccgt
     2461 cgttgctgtc gcgcagcact tgcagccgaa tttgcgcggt tttggagagc ggcgttttgt
     2521 agagcagcac gcagtgagaa gccaccgcac cacgacctac acgcccgcga agctggtgta
     2581 actgcgccag acccagacgc tccgggtttt cgataatcat cagactggcg ttaggcacat
     2641 caacgccgac ttcaataacg gttgtggcaa ccagcaggtg tagctcacct tgtttaaacg
     2701 acgccatcac cgcctgtttc tcggcaggtt tcatccgccc gtgtaccagg ccaacgttca
     2761 actctggtag cgccagtttc aactcttccc aggtagcttc cgccgcctgc gcttccagca
     2821 attccgactc ttcaatcaac gtacaaaccc agtatgcctg acgaccttca gttatgcagg
     2881 cgtggtgcac gcggtcaatg atgtcggtac ggcgggtatc aggaatagcg accgtagtca
     2941 ctggcgtgcg gcctggcggc agctcatcta tcaccgaggt atcgagatcg gcatacgcag
     3001 tcattgccag cgtgcggggg atcggcgtgg cggtcatgat caactgatgc ggatggaagc
     3061 cctgctgctg gcctttctcc cacaatgcca gacgctgatg cacgccaaaa cgatgctgtt
     3121 cgtcgataat caccagcgcc aggccgttaa actgcacctg ttcctggaag atggcgtgtg
     3181 taccgacaat catctgcacc tgaccgctgg cgatggcttc ctgctgtgcc agccgtgctt
     3241 tacctttctg cttaccggcg agccagccca cttcgatacc gagcggtgca aaccagttgc
     3301 ggaagttatt ggcgtgctgc tcggcgagta attcggttgg tgccatcaat gctacctgtt
     3361 tgccgtgggc aatcgcacgc aacgcggcga gggcggcgac cagcgtttta ccggaaccta
     3421 catcgccctg caccagacgc atcatcggca catccagcgc catatcgcgc tcgatctccg
     3481 ccactacgcg tgcctgtgcg cccgttggct tgaacggtaa ggcggcgagg agtttatttt
     3541 tcagcgtgtc attggcgctc agcggctggg catgaaaacg ctgtgctccg gcacgtaagg
     3601 ctaacatgct gaggttgtgc gccagcagtt cttccagaat cagacgacgt tgcgccggat
     3661 gctgcccggt ttccagatcg ctaagctgta gcgtcggtgg cgggcggtgc aaagtgcgca
     3721 acgcttccgg tagcgtcatc attccttgtg acagttccgg cggcaggagt tcttcaatgg
     3781 cgcaggtgtc gagcagatcc agcgcctggt cggttaattt acgcagcgtg gcctgcttta
     3841 cgccttccgt tgttggataa accggcgtga gcgtttcctg taattctggc gtgctgagat
     3901 cgccctgcac gcggtattcc gggtggatca tctccgcacc atatttaccg cgctttgctt
     3961 cgccataagc cagtacacgg cggcccgccg ccaggctatt tttcattgcc gcgctgaaat
     4021 tgaaaaagcg catggtgagg atgccggaac cgtcgctgat ctggcaggtc atcatccgcc
     4081 gaccgccgaa ggagatattg cagttcagca cttcgccttc caccgtggca taaacgcccg
     4141 gcagtagttc tccgatgggg tagagatggg tgcgatcttc gtagcgcagg ggaaggtgta
     4201 agagtagatc ctgcacggta tgcaggttga ttttcgccag tttgttacta agtgctgcgc
     4261 caacgcccgt tagggaactg agtgggacag catctaacag gcgacctttc atggcactta
     4321 ccctgcagcc tgcatagtag cccaccagtc ggcatcagct tcgatctcgc cttgctgatt
     4381 gacgtgggga taaggcaggc ctttgcgttt tgcgactttc gccagcaccg gatagccgcc
     4441 ttcaaacaac aggcgttgtt gctctgcttc cggcaacatg ctgttttcac gcaggtacat
     4501 gcctgcattt tgccgctgac gctgggcttc gtaaagaatg agggctgagg caacggacac
     4561 attcagcgac tgcaccatgc cgatcatcgg aatgatgatg tcctgatccg ccagggccaa
     4621 tgcttcctgc gtgatgcccg ttttctcctg tcccatcaaa atgcaggtcg ggcgagtgta
     4681 atcaatttcg cggaaatcga cagcgttatc agaaagatgg gttgccagaa tctgcatgcc
     4741 ctggcctttg agatgagcga cggcatcgcc aatggtgcgg tgtgttttca cctgtaccca
     4801 gctgttacta cccgccgctg ccgaagccat ggtgcgcatg cggctaccag gccagacggc
     4861 gtgaacttca tgtacgccaa cggcatctgc ggtacgaata atcgcagaaa cgttatgagg
     4921 tttgtggacc tgctccatgc agacggtcag atcaggctgc cgcctggcga gcatttcgca
     4981 gatgcgtgca taacgtgttg ggttcataaa acattaattt cggtttcggg tgactttaat
     5041 cacgtctggc atcacgcgga ttttgcgcat gatattcgcc agatgcacac ggtcacgagc
     5101 ggtcagacga ataaaggcgc tgtagacgcg accatctttc tcttccgtat tcaaactttg
     5161 aatattcgag gtcgtggtgt taattgccgc cgtcaggttt gccagcgcac cctgatgatt
     5221 gaacatctcc accttgattt cggtgatgaa ctcctgcgcc gtctctttat cccattccac
     5281 agccataaac ttctctggct ctttctggta gccacggatg ttacggcagg attcatggtg
     5341 gatcaccaga cctttaccgg ggctgacgtg ggcgataatc gggtcgccag gaatggggcg
     5401 gcagcatttc gcaaaggtga tcagcacgcc atcagcgcct ttgatcggca gatgcccgtg
     5461 gctttgagtc gccggtggaa tggaggcgtc cccatgttgt agatttttcg cgaccaccac
     5521 gctcattgcg ttaccaaggc cgatttctgc cagcagatcg tcaagcgttg ccagcttcat
     5581 gcgatccagc tcgcgctgaa tattttcctg cgggatttca ttgagcttac ggctaccacc
     5641 caaagcatgg ttgagcagac gacggcccag gcttacagaa tcatcacgct tgaggttttt
     5701 cagcaactga cgaattttgg cgcgcgcttt cgagctaacg acaaagttca gccaagcggc
     5761 attcgggcga gcgcccggag cggtaatgat ttcaacggtc tgaccgctag taagcggctg
     5821 cgacagcggg taaggctggc ggtcgacgcg tgcgcccacg caggcatggc cgatatcggt
     5881 atgcactgca taagcgaagt cgacgggcgt tgcaccggca ggcagctcga caatgcgccc
     5941 ttccggtgtg aaaacgtaaa tctcatccgg gaagagatcg gatttaacgc tctcgataaa
     6001 ttcaaacgaa ctaccggcgc tctgttgcag ctccagcagg ctttgcatcc agcgctgggc
     6061 gcggatttgc gcggtggtgc tggtttcgcc gtgctcttta taagcccagt gcgcggcaac
     6121 acccatctcc gccatctgat ccatatcttc ggtacggatc tggacctcaa ccggcacacc
     6181 gtgcgggccg atcatcgagg tgtgcaaaga ctgatagccg ttcgcttttg gaatggcgat
     6241 gtagtctttc acgcgacccg gacgcggctt gtacaggcta tgcatttgcc cgagcacgcg
     6301 ataacaggtg tcggaatcat tgacgatcac gcggaaagcg tagatgtcca tgattgagtg
     6361 aaaacgctgc tctttgagca ccattttgca gtaaatcgaa taaagatgct tctcgcgacc
     6421 actgacgcgg cacggaattc ccgcttcctg caaacgccct tcgatttcag agaggatttt
     6481 ttggatcatc tctttacggt tgccgcgcgc ggctttcacc acttctttaa ttacgcggta
     6541 acgattggga tacagcgcct caaaacccag ctcttcgagt tcggttttaa tgtggtggat
     6601 acctaaacgg tgcgccagcg ggctgtaaat ttcgagagtt tcacgggcga tgcggcgacg
     6661 tttgtccggg cgaagtgagc ccagcgtgcg catgttgtgg gtacggtcgg caagtttgat
     6721 gaggatgacg cggatatcct gcaccattgc cataatcatc ttgcgaaagt tttcggcctg
     6781 cgcctctttc ttatcacgga acttgagttt atcaagtttc gacaccccct ctaccagctc
     6841 ggcgacgctt ttaccaaaaa gctgttccat atcctggtag gtggcgggag tatcttcaat
     6901 cacgtcatgc agcagcgccg ccatcagcgt ttcatagtcg agtttcatct cggccagaat
     6961 gcaggcaacc gctaccgggt gcgtgatata gggttcaccg cttgaacgtg tttgcccctc
     7021 gtgagcatca cgtgcaacga gatacgcctg ccgcagacgc ttgatttggt cttccggcag
     7081 gtaggtttga atcagttgat tcaggctttc aaacagatac aagggcgacc cgctttgtga
     7141 ttaacgacga ccttcagcaa tagcggtaac ggcttgtaat tcagcggctt cctgctcttg
     7201 ctgttcctgg cgttcgcgaa cgtcgaggat ctggttgttg atcagacctt cttcgatttc
     7261 gcgcagcgcg attacagtgg ttttatcgtt ttcttccggt accagcggat cctttccgcc
     7321 tacctgcatc tgacgagcgc gacgcgcggc gaccagtacc aggtcaaaac ggttaccaat
     7381 tttctctaca gcgtcctgaa cagttacgcg tgccatactt aaaaagctcc acaggtgaag
     7441 aaatgactgg gcatgatact gaaatcaggt tcagtctgcc aacaatttgc tgattaaagc
     7501 gtcatgacgc tgcttttggc ggctcatgcg cagacgttcg gcgcgaataa tggtcttcaa
     7561 atcggtcaac gcggtatcga agtcatcatt cacaatcaga taatcatatt cggcgtaatg
     7621 gctcatttct gcaacagctt gcgccatacg ctttgcaatg acctcttcgc tgtcctgacc
     7681 gcgaccgcgt agacggcggt ccagttcaat tttggacggc ggtaaaataa agatactccg
     7741 cgcgtgcggc atcttctggc gaatttgctg cgcgccctgc cagtcgatat cgagaaaaac
     7801 atcgacaccg gtcgccagta cttgctcaat ggcctcacgc gaagtgccat agtaattacc
     7861 aaaaacttct gcgtgttcga ggaacgcatc tctgctaatc atttctttaa attcatcatg
     7921 attaacaaag aaataatgtt caccgtggac ttcaccagga cgcggttggc gtgtggtgtg
     7981 tgaaacagaa acctgggtgt catacaacgg ttgggttttt aataaagcct gaatcaggct
     8041 ggatttaccc gcgccactgg gggcagaaac aatataaagc gtgccttgag ccatgagtat
     8101 ctttcgtatg tgattagcga aataaagcct acatacgagc ttattataca cggcgcggcc
     8161 atgtgacgta gcccttgctc acactttttg cgcaaagttg ttgcgttttg cgtagctgtt
     8221 tccgcatttg cctgtgattt actgcaataa agacgattct caggaagcgt cctcgcagac
     8281 tccgtttttg ccactcggct ttgccttcac cacgttgtat atctgccagc ctgactacag
     8341 cggaggtgaa gatgaaagta tggatggcga tattaataag tatcttgtgc tggcaatcat
     8401 ctgtgtgggc ggtctgtccg gcctggtcgc cagccagagc acaggaagaa atttcccgcc
     8461 tgcaacagca aataaaacag tgggacgatg actactggaa ggaaggaaaa agtgaggtgg
     8521 aagacggtgt ttacgatcag ttaagcgccc gtcttacgca gtggcaacgc tgctttgtga
     8581 gcgagccccg cgacgtgatg atgccgcctt taaatggtgc ggttatgcat ccggttgccc
     8641 atacgggtgt acgcaaaatg gcggataaaa acgcattaag tctgtggatg cgagaacgta
     8701 gcgatctttg ggtgcagcca aaagttgatg gcgttgcggt aaccctggtt tatcgggacg
     8761 ggaaactgaa caaagcaatc agtcgcggta atggcctgaa aggcgaggac tggacgcaga
     8821 aagttagctt aatttccgct gtgccgcaaa ccgttagcgg gcctttagcc aacagtacgc
     8881 ttcaggggga aatatttctc cagcgcgagg ggcatatcca acaacaaatg gggggaataa
     8941 atgcccgcgc aaaagttgct ggcctgatga tgcgccagga cgatagcgac acgctgaatt
     9001 ctctgggcgt ttttgtctgg gcatggccgg atggaccgca gttaatgacc gatcgtttaa
     9061 aggagctggc taccgcaggt tttactctaa cgcagaggta tacccgtgcg gttaaaaatg
     9121 ctgatgaggt tgcgcgcgta cgcaatgagt ggtggaaagc gaaattaccc ttcgtcaccg
     9181 atggcgtggt tgtgcgaggg gcgaaagaac ccgaatcccg ccattggcta ccaggccagg
     9241 cagaatggct ggtggcctgg aaatatcaac ctgtagctca ggttgccgaa gtgaaggcga
     9301 ttcagtttgc ggtgggtaag agcggtaaaa tatcggtggt tgcgtcactc gcacctgtca
     9361 tgctggatga taaaaaagtc cagcgggtga atattggttc cgtcaggcgc tggcaggagt
     9421 gggatattgc gcctggtgat cagatcctcg tcagccttgc cggtcagggt attcctcgca
     9481 ttgatgatgt ggtctggcgc ggtgcagaac gtacaaaacc gacaccacca gaaaaccgct
     9541 ttaactcgtt gacctgctac tttgcttctg atgtttgtca ggaacagttc atttcacgct
     9601 tagtctggct ggggtcaaaa caggttctgg ggctggatgg tattggtgag gccggctggc
     9661 gcgcgctgca tcagactcat cgctttgagc atatcttttc ctggctttta ttaacgccag
     9721 agcaattaca gaacacgccg gggatcgcga aaagtaaaag tgcgcagcta tggcatcggt
     9781 ttaatctggc tcgtaagcag ccttttactc gctgggtgat ggcaatggga ataccgctaa
     9841 cccgggcggc gcttaatgcc agtgatgaac ggtcctggtc gcaactttta tttagcacgg
     9901 agcagttctg gcagcagctg ccggggactg gatcggggcg cgccagacag gttattgaat
     9961 ggaaggaaaa tgcgcaaatc aagaagctgg gcagttggct ggctgcccag cagatcacag
    10021 gttttgaacc ttagtgtcct tcgtggctgt agtaaaagac cggtaatccc agcttaaggc
    10081 gtagcgccag taagcgggca aaaaagccga ataccagggt ggagataatt accacatcat
    10141 ggttagaaac atagtgttgc agcgcgatgt acagcacggc ggaggcaaat gagacaccgg
    10201 catacaactc tttctggaat accagtggga tacgtttaca gaacatatcg cgcaaaacgc
    10261 cgccaaacac gccggtggtt accgccgcga caacggcaat aattgggccg tgccccatat
    10321 ccagtgcaac ctgtgcgccg atgatagaaa agaccaccag tccaagcgca tcgagcacca
    10381 gaaacacttt gcgcaggtaa ggcattacag gagcaacgat agtggtgagt actgcggcgg
    10441 tagcgacgat aatcacatat tccgggtgtt tgacccagcc gagcggatag tggcccagca
    10501 gaatatcgcg cactgaccct ccgccaattg cggtggcggt cgcaataata attacgccaa
    10561 atgtatccat gcggcgtcgc ccggccgcca gagcccccgt catggcttcg gcagtaatgc
    10621 caaccaaata caaaatgtgt aacagcatgg ttctctccct aaaaatctgt ctggcagggt
    10681 agcgatttgt gcgtattgtc acgattgaga ttttttaagg tgagaggaaa ttgataactt
    10741 gtagttatgt gaaatcgcta ttttctgtag cgtagatgca ttcttctgac ttcttgtctg
    10801 agaaaatgca taagaaaaaa taggttaaaa ttgaggggtg gaaaagaaag gtgcaatgtt
    10861 tagccattgt gttcaccgca cctggacttt ggcttacgca tcgggtatct caatcaacaa
    10921 aaagagtttt tagtttctgc tatttcttct ctgtaatttt tacactgttt tccaactgct
    10981 tgatgctgac ctgcggggtc ggcaactcct caggcatggt tccgccaagt tcctgaatgg
    11041 tttgcctcac cttgctaccc acgtcaaagt gtgtggtatt agcttgctgt tttgaattca
    11101 cgccatcccg cttgagtttt tcttctgttt gggtagctcg aaagagatta gccgccagtt
    11161 ctgttgaacc catatgatcc aggatcttct gactcttttt cagccccttc cgctgatgga
    11221 tggctttctg atctaatcca ccatacagcc cccggtaacc atgattctga aatatggcga
    11281 aatcgatagc tgtcgctacc ccagcttgct gcgctgcctc aaccaattgt ttgttatgtt
    11341 ctttcaattc atttctcaga aaaagacgct tttcgtcttc acgaagttgc ctgaaagctt
    11401 catcgtcagc cagctcctgc cgtcgggtct ggatagcaaa ataagtttgc cctgccgcaa
    11461 taaccggttt cgcagggtcg ccgttttgta ctaccaggta acaggcatag cgggagagat
    11521 gaacatcttc aagttctcgt tgagcccctg aacctaatac aaccattttg gtggtttcaa
    11581 cgaaatggtc agatgcagct tgattgctgg cttcgcacgc ttgagtcgcg cgggccagta
    11641 ccttttgaaa attgcgccag tcccgataat ccagtaacgg tgccaattct ctagctgacc
    11701 aaaattcttg cccttcagtg ccataatgcc gtatttcttc aaaaggttga tgatgttcgt
    11761 tcattgccca ctccatgtga tatcgattgc aaagaaagta aatctgtaac tgtatttata
    11821 tacagttgtg agtatctcac gttctcagtg tgctgggcaa aaaaacatcg caaaatctgg
    11881 agcaggcacg caaaacagaa ttgaagttgc ggggaagaaa aatgccgctc tgtttgagtg
    11941 ggcggcattt gtgaaggctg aaaagtaaga attagtgtta cgagttactc aatattctga
    12001 atctgctcgc gcatctgctc aatcaacact ttcagctcaa tcgcggagtt tgtcacttcg
    12061 gcattgatag acttcgacgc aagagtgttc gactcgcggt tgaattcctg catcataaag
    12121 tccagacgac gaccaacggc ttcttttttc ttcagaatgt tgtaggtctc tttgacatgc
    12181 gcttccaggc ggtccagttc ttcggcaacg tcaattcgtt gtgccagcag aaccaactcc
    12241 tgctccaggc ggttgttttc cagttgcact tgagcatctt ccagcttcgc gaccagacgc
    12301 tcacgctgcc attgcaggat ttccggcata tgggcgcgga ctttgaccac ttcggcggtg
    12361 acgccttcca gacgctgctc gatcaatgct ttcagcgcct gaccttcggt ttcacgtgcg
    12421 acgataaagt cgtccagggt accatccaac gccgcgagaa tttcagcggc aatcgcgtcg
    12481 agatcctgct cctgggctgc cattacgccc ggccagcgca gaatatcaac cgggttgatt
    12541 tccccttcgt cactctgcat ttttacccag ttcgcggcag ttaccagctg tttagccagt
    12601 ttttcgttga ggatcagctc accttgcgcg ctaacatctg gctcatagcg cagggtacat
    12661 tccactttac cgcgcgtcag gcgagaacga atacgctcgc gaacgacagg ttcaaggcta
    12721 cggaactgct ccggcagacg aaagtaagtt tccagataac gctggtttac cgagcgcatt
    12781 tcccaggttg cgctccccca ttcacccttg atttcacgcc gggcgtaggc ggtcatactg
    12841 cggatcatag acgttcctgt ttataaaagg agaggtggaa ggattatagc catcgatgcc
    12901 ttgtcaggat aggaataacc gccggaagtc cgtataatgc gcagccacat ttgtttcaag
    12961 ccggagattt caatatgcgt ccagcaggcc gtagcaataa tcaggtgcgt cccgttaccc
    13021 tgactcgtaa ctatacaaaa catgcagaag gctcggtgct ggtcgaattt ggcgatacca
    13081 aagtgttgtg taccgcctct attgaagaag gcgtgccgcg cttcctgaaa ggtcagggcc
    13141 agggctggat caccgcagag tacggcatgc tgccacgttc tacccacacc cgtaacgctc
    13201 gtgaagcggc gaaaggtaag cagggtggac gcacaatgga aatccagcgt ctgatcgccc
    13261 gtgctcttcg cgcggcagta gatttgaaag cgctgggtga gttcaccatt acgctggact
    13321 gcgacgtgct tcaggctgat ggtggcacgc gtaccgcgtc gattacgggt gcctgcgtgg
    13381 cgctggcaga tgcgctacag aagctggtgg aaaacggcaa gctgaaaacc aacccgatga
    13441 aagggatggt agccgcagtt tctgtcggta ttgtaaacgg tgaagcaatt tgcgatctgg
    13501 aatacgttga agactcagcc gcagagaccg acatgaacgt agtgatgacc gaagacgggc
    13561 gcatcattga agtgcagggg acggcagaag gcgagccgtt cacccatgaa gagctactca
    13621 ccttgttggc gctggcccga gggggaatcg aatccattgt agcgacgcag aaggcggcgc
    13681 tggcaaactg atttttaagg cgactgatga gtcgcctttt ttttgtctgt agaaaagtaa
    13741 gatgaggagc gaaggcatga aaccatatca gcgccagttt attgaatttg cgcttggcaa
    13801 gcaggtgtta aagtttggcg agtttacgtt gaagtccggg cgcaaaagcc cctatttctt
    13861 caacgccggg ctgtttaata ccgggcgcga tctggcactg ttaggccgtt tttacgctga
    13921 agcgttggtg gattccggcc ttgagttcga tctgctgttt ggccctgctt acaaagggat
    13981 cccgattgcc accactaccg ccgtggcgct ggcggagcat catgaccttg acctgccgta
    14041 ctgctttaac cgcaaagagg caaaagacca cggtgaaggc ggcaatctgg ttggtagcgc
    14101 gttacaagga cgcgtaatgc tggtagatga tgtgatcacc gccggaacgg cgattcgcga
    14161 atcgatggag attattcagg ctaatggcgc gacgcttgct ggcgtgttga tttcgcttga
    14221 tcgtcaggaa cgggggcgcg gcgagatttc ggcgattcag gaagttgagc gtgattacaa
    14281 ctgcaaagtg atctctatca tcaccctgaa agacctgatt gcttacctgg aagagaagcc
    14341 ggaaatggcg gaacatctgg cggcggttaa ggcctatcgc gaagagtttg gcgtttaaag
    14401 aaactcgccg gatgaaaagt catccggcgt catattactg caactgtgcc gcaattagcg
    14461 gccagcgggc gtcaaaatca tccgtcgggc ggtatttaaa ttcgctgcgg acaaaacgtg
    14521 acagcatacc ttcacagaag gccaggatct ggcttgccag cagggtttca tcggtggtgt
    14581 aaccttcacc ctcacgcatt ctcttttcac gcaatacctg gcgcagctgc gcttcaatac
    14641 gctcgaacag ctggttgatg cgcccttgca ggcgatcctg ttcaaacatt agcgcatgac
    14701 cagtgaggat gcgggtcagg ccaggattac gctcaccaaa accgagaagc agcaacacaa
    14761 tcagacgcag gcgcgctgtg gtgtctttct catctttcag aatcaggttg atgcgagtaa
    14821 tcaggctatc ttcgataaac tcaatcaggc tatcgaacat gcgggtctta ctggggaagt
    14881 ggcgatacag tgccgcttcg gaaacgccga cagaggcggc cagttttgcc gtcgtgatac
    14941 gttggcttcc atcgctggat tccagcatca gcgccagaga ctgaagtatt tcctcgcgac
    15001 ggttcctttt cgcagtttgt ttttctgcca tgttacaaaa tacccctgaa aataagcact
    15061 tgccaggcgg cacccacgct atgaccgcaa acgaaatgtt tgcggctatg ttatgacgtt
    15121 attcggatgc gtatgtgtta ctgacgacca gagtgaccaa agccgccttc accgcggtcg
    15181 gtggcgtcga aatcttccac cagattaaat tcagcctgta ctaccggaac aaaaatcatc
    15241 tgggcgatgc gttcgccagg ttgaatggtg aagctgtcct gaccacggtt ccacacggaa
    15301 atcatcaact ggccctgata gtcagaatcg atcaatccta ccaggttacc aagcacgata
    15361 ccgtgcttat gtcccaatcc ggagcgcggc agcatcattg ccgccagtga aggatcggca
    15421 atatgaatcg ccagcccggt cggaaccagc gtagtgtcac ccggagccag ttctacggcg
    15481 tcgtcgagac aggcacgcag gtcaagtccg gcagagccag aggtggcata agtcgggagc
    15541 ggaaattcct tcccaacgcg cgggtccaga atcttaacgt cgattttttt catcataacg
    15601 ggtcacgatc tcgtcgagta ataattggcc aaggagctct ttgcgctcaa gcggtaagac
    15661 tttatctccg tcctgccaga aaaggtgtaa tgcgttgttg tcgctgttaa atccttgagt
    15721 tggctgggaa acatcgttcg cgcagatcag atcaaggttt ttacggatac gtttttgccg
    15781 ggcgtattct tccacattat ttgtttcggc ggcaaatcca acgacgtagg gtcgatggtc
    15841 ttttagtgcg gcaacgcctg cgacgatatc ggggttttta accattttta ttgttaattc
    15901 atcaccctgc gtggcctgct ttttgatttt ctctggggcc acggtagctg cgcgataatc
    15961 cgccacggcg gcgcagccga taaaaatatt ttgctgctgt acagaagcat tcacggcggc
    16021 ttccatttcc agcgcagtca tcacatcaac acgtttaaca aacggtggcg tcggtagtga
    16081 aaccggacct gataccagcg tgacgttcgc gccacgacgg gcagcggcgg cggcgatagc
    16141 aaaacccatc ttgccggagc tgtgattaga gatataacgc accggatcga gcggttcacg
    16201 cgtcggaccg gcggtaatca taatgttcag atgtttcagg tcgttgacgg gcgaaaaatg
    16261 cgccaccgcc atatccacaa tggttaacgg atcgagcatt cgcccaggac cgatatcacc
    16321 acaagcctga ctgccactgt ctggccccca gatgagcaaa ccacgggaag caagcacctc
    16381 taaattatgc tgcgtggcag cggcacggta catctgctgg ttcatggcgg ggagcacggc
    16441 tacaggcgca ggtgtagcca gacaaatcgt cgataccagg tcattcgcca ttccggcagc
    16501 aacacgggca atcaaatctg ccgtggcagg ggcgagaatc actaaatcag cccatttacc
    16561 cagctcaata tggcccatag cggcttcggc tgccgggtcc agcaaactgt cggaaacggg
    16621 ataaccagaa accgcctgca agctaagtgg ggtgataaag gcttttgccg cttcggtcat
    16681 ggctacgcgg acgtcggccc cgcgatcgcg caaacgacgc accagttcag gggttttata
    16741 ggcagcaata ccgccgctaa cgccgagaac gattttttta ccggccaggc tcatgatgat
    16801 ttttcctgtt gggtgacacc agaagttggc gattttatca caatactttt gttgtcgtgc
    16861 cttcacctga gaatcacttt gcgaggcgct ttccaggatt gaaaactggc cgtcgattta
    16921 acggaacggc tatgacagga tgcgagcacc acaaaggagg tgaaggtgaa aaacaatgca
    16981 cagctgttga tgccgcgcga aaaaatgctg aagtttggta ttagcgcctt aacggatgtc
    17041 gagctgctgg cgttatttct gcgtaccgga acgcgcggta aagatgtatt aaccctggca
    17101 aaagagatgc tggagaattt cggctctctt tatggcttgt taacctctga atatgaacaa
    17161 tttagtggcg ttcatggaat tggcgtagcg aaatttgccc agttaaaggg gattgctgaa
    17221 ctggcgcggc gttactacaa cgtgcggatg cgtgaagaaa gccctttact cagcccggag
    17281 atgacgcggg aatttttaca aagccagctc acgggtgagg agcgggagat ctttatggtg
    17341 atctttctcg actcacaaca ccgggttata acgcatagcc gtcttttttc cggcacgcta
    17401 aaccatgttg aagtccatcc tcgggaaatt atccgcgaag cgataaaaat aaacgcctcg
    17461 gcgctgatcc ttgcacataa tcacccttcg ggttgtgctg aacccagtaa agcggataaa
    17521 ctcattactg aacggataat aaagagttgt cagttcatgg atttacgcgt gctcgaccat
    17581 atcgtgattg ggcgtggaga gtatgtttct tttgccgaac gcggctggat ttaacccgct
    17641 atgcgcgatc cttcgggatc tttgtctgtt cgggacttga gcacatcgct gagtcagcgt
    17701 atactacgcc acctttgaga atctcgggtt tggcatttgg gcctggcaat cgagagttca
    17761 catagaactg cgatgaccgg gctgtaaagc ctgacgaggc gccaataccc catacgaagc
    17821 tcgagctaat ttgatttttg gagaatagac atgtcccgag tctgccaagt tactggcaag
    17881 cgtccggtga ccggtaacaa ccgttcccac gcactgaacg cgactaaacg ccgtttcctg
    17941 ccgaacctgc actctcaccg tttctgggtt gagagcgaga agcgttttgt caccctgcgc
    18001 gtatctgcta aaggtatgcg tgtaatcgat aaaaaaggca tcgatacagt tctggctgaa
    18061 ctgcgtgccc gtggcgaaaa gtactaagta cttagaggaa ataaatcatg gctaaaggta
    18121 ttcgtgagaa aatcaagctg gtttcttctg ctggtactgg tcacttctat accactacga
    18181 agaacaaacg tactaagccg gaaaaactgg aactgaaaaa attcgatcca gttgttcgtc
    18241 agcacgtgat ctacaaagaa gcgaaaatca aataattctc gctttgatgt aacaaaaaac
    18301 ctcgctccgg cggggttttt tgttatctgc ttgcccccat attgattgca tctgttcatt
    18361 cctggagatg ctatgcctga attacccgaa gttgaaacca gccgtcgcgg catagaaccg
    18421 catctcgttg gtgcaaccat tcttcatgca gtggtgcgca acggacgctt gcgctggccg
    18481 gtttcagaag agatctaccg tttaagcgac caaccagtgc ttagcgtgca gcggcgggct
    18541 aaatatttgc tgctggagct gcctgagggc tggattatca ttcatttggg gatgtctggc
    18601 agcctgcgca tccttccaga agaacttccc cctgaaaagc atgaccatgt ggatttggtg
    18661 atgagcaacg gtaaagtgct gcgctacacc gatccgcgcc gctttggtgc ctggctgtgg
    18721 accaaagagc tggaagggca taatgtgctg gcccatcttg gaccggagcc gctcagcgac
    18781 gatttcaatg gtgagtatct gcatcagaag tacgcgaaga aaaaaacggc gattaaaccg
    18841 tggctgatgg ataacaagct ggtggtaggg gtagggaata tctatgccag cgaatcactg
    18901 tttgcggcgg ggatccatcc ggatcggctg gcgtcatcac tgtcgctggc agagtgtgaa
    18961 ttgttagctc gggtgattaa agcggtgttg ctgcgttcga ttgagcaggg cggtacaacg
    19021 ctgaaagatt ttctgcaaag tgatggtaaa ccgggctatt tcgcacagga attgcaggtt
    19081 tacgggcgaa aaggcgagcc gtgtcgggtg tgcggtacgc cgattgtggc gactaaacat
    19141 gcgcagcggg caacgtttta ttgtcggcag tgccagaagt aattcatgcg cgccggatgg
    19201 cataccatcc ggcataaacg ctacgctaac ttcgccatca gcgcctgatg gacattctcc
    19261 ggcaggaaat gggtgacatc gccctgatgg cgcgccacct ctttcaccaa cgatgaagag
    19321 ataaacgacc actctttcga cggcatcaga aacacacttt ccagttccgg cattaagtgg
    19381 cgattcatat gcgccagctg catttcatat tcaaaatccg ccaccgcacg caggccacga
    19441 atcagcaccg tagcgtgttg attacgggcg aagttcgcca tcaaatcact aaacccgacc
    19501 acttccacgt tccccagatg cgcggttgcc tgctgtgcca gcgccacacg ctcttccagg
    19561 gtaaacatcg gttttttact ggggctggcg gcaatcgcca gaataacgtg atcgaacatt
    19621 tgcgtggcgc gcgtcacgat atcgatatga ccattggtaa tgggatcgaa agtacccgga
    19681 taaatcgccc gtttttgcat aacaacctca atgcgttttc ggtggcaggt aaggttccag
    19741 cagttgaagc agacgctgta gcgcgccctg gttttgatac agtacttcaa cggcatgacg
    19801 gccatagaaa ctacggtaat cggcgtcggt gagtaaagag gacacctctt ttgcaagcgt
    19861 agtggcatcg gtaacggtaa tcagcccgct tgcctgctcc agccgcgcgc aaatgtcttt
    19921 aaagttaaaa gtatgcggcc ccatcaatac cggaatagcg tgtgcggcag cttccagcgg
    19981 attatgccca ccacgttcaa ccagtgaacc gccaacaaag gcgagatcgg caatgccgta
    20041 cagtaacatc aactcgccca tcgtatcgcc aaccacaacc tgcgtgctgg tggaggggac
    20101 ttcccctgaa gagcgtgtga tatagcttag tccagcctgg cggacaagat taatcgcatc
    20161 cgggaagcgt tctggatgac ggggtaccag gatgagcaat aaattcggga attgctgtaa
    20221 caatgcctga tgtgcggcga tcaccacact ctcttcgcct tcgtgagtgc tggtggcaat
    20281 ccataccggg cggtgtggtg cccactggcg gcgcagcgtc acggctttag cagccaactg
    20341 cggcgttaca gaaatatcga atttcaggct accggtaacg gtcacctgat tattttttgc
    20401 gcccagcgcc acaaaacgtg caccatcttc ttcattttgc gcagcaatca gcgtaatacg
    20461 acgcagcaag cgacggacga atttacccag tttggcataa cctgcggccg agcgggcaga
    20521 gagtcgcgcg ttagcgatca ccagcggaat tttacgtttg tgtagcgccg caatcaggtt
    20581 aggccatagt tcggtttcca taatcaacac cagtttgggg tcgactttat tcaggaaacg
    20641 gttgagcgca tcgggcaggt catacggcag ataaacgtgc tgaacatcct tcccgaaagc
    20701 cgattgtacg cgctccgaac cggttggcgt catggttgta acggtaatcg gtaaatcagg
    20761 ataacgatgg cgcagcgcgc gcaccaacgg gatcgccgcc agagtttcac cgacggagac
    20821 ggagtgcagc ataatgccgc ctggttttag cggatggcgg taaaaaccgt aacgttcacc
    20881 ccagcgtttt cgataggccg gagccttacg tccgcgcacc cagagccgta tccagatcag
    20941 cggctgaata aggtagagaa gggcggtgta aagcaattcg agcatagtaa atagctgact
    21001 tatggatgtg ctggggattc tatgtattta gctgtggctt taccattact tttcccgttt
    21061 ttgacttaaa tagcaattta cccgccaatt cttgctgaac atctatactt tttttgtatt
    21121 tttatgcgat tcattgattt ctgctgtacg tgcaggaagg aatcgcagag caacagtgaa
    21181 agaatatggt tgctaatgat tttatttgat tgatttcttt attgaaaatc aacaatgtgc
    21241 ttaaaaatca ttcgggtcgg attgccgcga aagaagtgat acactagcgc gtcaatatca
    21301 gtgcggtatc agtattcagg tagctgttga gcctggggcg gtagcgtgct ttttctgctt
    21361 caccttaatc ggataatctc aacaaaaaga gttacttgtg gataagccat ttcgaagaat
    21421 tttgctcatt aagatgcgtt ttcatgggga tatgttatta actactcccg tcattagttc
    21481 gctgaaaaaa aattaccctg acgcaaaaat cgatgtgctg ctttatcagg acaccatccc
    21541 gatcctgtct gaaaatccag agattaacgc gctctacggc ataaaaaata aaaaagcaaa
    21601 agcctcagaa aaaattgcca acttttttca tctcatcaag gtattacgtg ccaataagta
    21661 tgaccttatc gtcaatctca ccgatcaatg gatggttgct atactggttc gcttattaaa
    21721 tgcccgtgtg aaaatttccc aggattatca tcatcggcag tctgcttttt ggcgtaaaag
    21781 tttcacccat ttggtgccgt tgcagggtgg aaatgtggtg gaaagtaact tatccgtgct
    21841 gaccccattg ggagttgatt cgttggtgaa gcagacaacc atgagttacc cgcctgcaag
    21901 ctggaaacgt atgcgtcgcg aacttgatca cgctggtgtt ggacaaaatt atgtggttat
    21961 ccaacctacg gcgcggcaaa tcttcaaatg ctgggacaac gccaagtttt ccgctgtgat
    22021 tgatgcctta catgctcgtg gttatgaagt cgttctgacg tccggcccag ataaagacga
    22081 tctggcctgc gtcaatgaaa ttgcgcaggg atgccagacg ccaccagtaa cggcgctggc
    22141 tggaaaggtg accttcccgg aacttggtgc gttaatcgat catgcgcagc tgtttattgg
    22201 cgttgattcc gcaccggcgc atattgccgc tgcagttaat acgccgctga tatcgctgtt
    22261 tggtgcgaca gatcatattt tctggcgtcc ctggtcaaat aacatgattc aattctgggc
    22321 gggagattac cgggaaatgc caacgcgcga tcagcgtgac cgaaatgaga tgtatctttc
    22381 ggttattccg gcggcagatg tcattgctgc tgtcgataaa ttactgccct cctccacgac
    22441 aggtacgtcg ttatgatcgt tgctttttgt ttatataaat attttccctt tggcggtttg
    22501 cagcgtgatt ttatgcgtat tgctcagaca gtcgccgccc gaggtcatca tgttcgggtt
    22561 tatacccagt cgtgggaagg cgaatgccct gatgtatttg aactgatcaa agtgccggtt
    22621 aaatcgcata ccaatcacgg gcgcaatgcg gagtattttg cctgggtgca aaaacattta
    22681 cgcgaacatc ccgtcgataa agtcgttgga ttcaacaaaa tgccggggct ggacgtttat
    22741 tatgccgctg atgtttgtta tgccgagaaa gtagcgcagg aaaaaggctt tttctatcgc
    22801 ctgacgtcac gttatcgcca ttatgccgcc tttgagcggg caaccttcga acagggtaag
    22861 ccgacacagc tgctgatgct gacagataag caaatcgccg atttccagaa acattatcag
    22921 actgaagcgg agcgttttca tattctgcca ccggggattt atcctgatcg taaatatagc
    22981 cagcagccag ccaatagccg tgaaatcttc cgtaagaaga atggaataac cgaacaacaa
    23041 tatttattgt tgcaggtcgg ttcagacttc acgcgtaaag gtgtcgatcg ttccattgaa
    23101 gcacttgctt cgttaccgga ttcgctgcgc cacaacacat tgctatatgt tgttgggcag
    23161 gataaaccgc gaaaatttga ggcgctggca gaaaaacgcg gcgtgtgcag taatgttcac
    23221 tttttctcgg ggcgcaatga tgtctcggaa ttaatggcgg cggcggattt attactgcat
    23281 cctgcctacc aggaagcggc gggaattgtg ctgctggaag cgattactgc aggattaccg
    23341 gtactaacaa cagccgtttg tggctatgcg ccttatattg tcgaggctaa ttgcggcgag
    23401 gctattgctg agccattccg ccaggaaaca ttgaatgaga ttttacgcaa agcgttaacg
    23461 caatcttcat tgcgccaggc ctgggcggaa aatgcgcgat attatgctga tacacaagat
    23521 ttatacagtc tgccagagaa agcggcggat atcataacgg gtggtctgga tggttgaact
    23581 taaagagccg tttgccacgt tatggcgcgg caaagatcct tttgaggaag ttaaaacctt
    23641 gcagggtgag gtatttcgtg aactggaaac tcgccgtact ctgcgctttg aaatggcggg
    23701 caaaagctat tttctcaaat ggcatcgcgg cacgaccctg aaagagataa tcaaaaattt
    23761 actctcattg cggatgccag tattaggcgc agaccgcgaa tggaatgcga ttcatcgact
    23821 gcgggatgtc ggcgttgata ctatgcatgg ggtggcattt ggcgaaaaag gcatgaatcc
    23881 gctgaccaga acttcattta ttattaccga agatctgaca ccaaccataa gtctggaaga
    23941 ttactgtgct gactgggcga ctaaccctcc agatgttcgc gtaaagcgta tgcttattaa
    24001 gcgtgtcgcg acgatggtgc gcgatatgca tgctgcgggc attaaccacc gtgactgtta
    24061 tatctgtcat ttcctgctgc acttgccttt ttccggtaag gaagaggagt taaaaatttc
    24121 ggtaattgac ctgcaccggg cgcagcttcg cacgcgcgtt ccacgtcgtt ggcgggataa
    24181 agatcttatt gggctttatt tttcttcgat gaatatcggc ctgactcagc gggatatctg
    24241 gcggtttatg aaagtgtatt ttgccgcccc gcttaaagac attctcaagc aggaacaagg
    24301 actgctgtcg caagcagaag caaaagccac aaaaatcagg gaaagaacga ttcgaaaatc
    24361 gttgtaaccc gtaacttatt tttgccaaaa ttttggatac agaataaata
//
LOCUS       DAZKHF020000049        23439 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2201S31241
            SAMN43267800-rid25106413.denovo.049, whole genome shotgun sequence.
ACCESSION   DAZKHF020000049 DAZKHF020000000
VERSION     DAZKHF020000049.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267800
            Sequence Read Archive: SRR30307263
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 23439)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23439)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 23439)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350581.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350581.2
            Genome Coverage       :: 151x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:21:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,652
            CDSs (total)                      :: 5,557
            Genes (coding)                    :: 5,320
            CDSs (with protein)               :: 5,320
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 237
            CDSs (without protein)            :: 237
            Pseudo Genes (ambiguous residues) :: 0 of 237
            Pseudo Genes (frameshifted)       :: 102 of 237
            Pseudo Genes (incomplete)         :: 130 of 237
            Pseudo Genes (internal stop)      :: 52 of 237
            Pseudo Genes (multiple problems)  :: 41 of 237
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23439
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267800-rid25106413.denovo.049"
                     /serotype="O 26:H11"
                     /isolate="2201S31241"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-01"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            <1..701
                     /locus_tag="ACHOSC_RS07885"
                     /old_locus_tag="pgaptmp_001577"
     CDS             <1..701
                     /locus_tag="ACHOSC_RS07885"
                     /old_locus_tag="pgaptmp_001577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000225871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="fimbria/pilus periplasmic chaperone"
                     /protein_id="HGU4547543.1"
                     /translation="LLSRLTPLLFVFAPFLAQSNMTVYPMAVSINSQGEGNVRVISKS
                     NEVQYIKATVFRIDNPSTPQENEVEIKSGDANHLVVMPPKFALPAGSSKTVRFVAMEP
                     EQKEKNYRVKFEAVPSIDDVATDKKDLSMQLTVNLIWGIVVSVPPQQPIAKLEVNAAQ
                     KLVNAGNQRLKILTIAYCKNNSKENCKIQTVNKNIFPGQERNLESISGYDKIVVKYNN
                     WITKDNGEFELAVH"
     gene            731..1231
                     /locus_tag="ACHOSC_RS07890"
                     /old_locus_tag="pgaptmp_001578"
     CDS             731..1231
                     /locus_tag="ACHOSC_RS07890"
                     /old_locus_tag="pgaptmp_001578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000755097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CS1 type fimbrial major subunit"
                     /protein_id="HGU4547544.1"
                     /translation="MKKVFAKSLLVAAMFSVAGSALAVQKDITVTANVDAALDMTQTD
                     NTALPKAVEMQYLPGQGLQSYQLMTKIWSNDVTKDVKMQLVSPAQLVQSLDASKIVPL
                     TVTWGGEEIKADAATTFTATKIFASDALTNGSLAKNLMFAQITKGVLETGIYRGVVSI
                     YLSQDI"
     gene            1323..4007
                     /locus_tag="ACHOSC_RS07895"
                     /old_locus_tag="pgaptmp_001579"
     CDS             1323..4007
                     /locus_tag="ACHOSC_RS07895"
                     /old_locus_tag="pgaptmp_001579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001666746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TcfC E-set like domain-containing protein"
                     /protein_id="HGU4547545.1"
                     /translation="MDKKLLALLILASLSPAEATLTKIPAGFEVIAQGQQEYIEVYFS
                     GKSLGKYYAMVNLDTVTFLDPASLYNKLELDVDDQKIAHIVKEKLSQPLARHGELACG
                     YVRTDSGCGFLNTDTLEIIYNDEESSATLFINPQWNSAFDAKSLYLNPDKNTVNAFIH
                     QQDINVLAQDDYQSLSIQGNGALGITENSYIGAHWNFNGYDADDVSDSNADISDLYYR
                     YDFLRRYYVQAGRMDNRTLFNAQGGNFTFNFLPLGAIDGMRIGSTLSYLNQAQSQQGT
                     PVMVLLSRNSRVDAYRNEQLLGSFYLNSGSQFIDTSSFPPGSYSVALKVYENNQLTRT
                     ELVPFTKTGGLTDGNAQWFLQAGKTTSQVSDDESSAYQLGVRLPLHPQYELYAGLANA
                     DDVSAFELGNNWTADLGGVGNLAISASVFRNDDGGKGDMQQANWSNPGWPTLGFYRTN
                     SDGDACTTDSRESYNALSCYESISATVSQNFVGWNMMLGYTRTQNNTDDSLRWDKQQS
                     FENNYLRQTTAQSISETVQLSASRAFVMRDWILSTSVGVFHRNDNGGDNDDNGLYLSF
                     SLSDTPTMDSNNNSHSTNVSTDYRYSEQDGDQTSWQLSHTFYNDSFSHKELGVTVGGL
                     NTDTINSAVNGRWDGQYGNVYATVSDSYDRKNHDHLSAFTGTYSSTLAVSRYGVNLGA
                     SGTDGLLGAVLVDVKGFSEQDEESQDLQLEARVAGSRTLQLGQSDSVLFPYPGFQSGF
                     VEVNDSSQGNQQGTTNIINGAGNRELMLLPGKLRYREVSASFNYNYIGRLLLPAAVKK
                     FPIVGLNSAMLLVAEDGGFTLEINGSEKELYLLSGQQFLKCPLSVVKKRASIRYSGDV
                     TCSVVTYSQLPESIQVQAQLKQPKLRGNVQTAQREVAP"
     gene            4004..5078
                     /locus_tag="ACHOSC_RS07900"
                     /old_locus_tag="pgaptmp_001580"
                     /pseudo
     CDS             4004..5078
                     /locus_tag="ACHOSC_RS07900"
                     /old_locus_tag="pgaptmp_001580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001352530.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="CfaE/CblD family pilus tip adhesin"
     gene            complement(5166..6464)
                     /gene="exuT"
                     /locus_tag="ACHOSC_RS07905"
                     /old_locus_tag="pgaptmp_001581"
     CDS             complement(5166..6464)
                     /gene="exuT"
                     /locus_tag="ACHOSC_RS07905"
                     /old_locus_tag="pgaptmp_001581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708898.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hexuronate transporter ExuT"
                     /protein_id="HGU4547546.1"
                     /translation="MRKIKGLRWYMIALVTLGTVLGYLTRNTVAAAAPTLMEELNIST
                     QQYSYIIAAYSAAYTVMQPVAGYVLDVLGTKIGYAMFAVLWAVFCGATALAGSWGGLA
                     IARGAVGAAEAAMIPAGLKASSEWFPAKERSIAVGYFNVGSSIGAMIAPPLVVWAIVM
                     HSWQMAFIISGALSFIWAMAWLIFYKHPRDQKHLTDEERDYIINGQEAQHQVSTAKKM
                     SVGQILRNRQFWGIALPRFLAEPAWGTFNAWIPLFMFKVYGFNLKEIAMFAWMPMLFA
                     DLGCILGGYLPPLFQRWFGVNLIVSRKMVVTLGAVLMIGPGMIGLFTNPYVAIMLLCI
                     GGFAHQALSGALITLSSDVFGRNEVATANGLTGMSAWLASTLFALVVGALADTIGFSP
                     LFAVLAVFDLLGALVIWTVLQNKPAIEVAQETHNDPAPQH"
     gene            6947..8359
                     /gene="uxaC"
                     /locus_tag="ACHOSC_RS07910"
                     /old_locus_tag="pgaptmp_001582"
     CDS             6947..8359
                     /gene="uxaC"
                     /locus_tag="ACHOSC_RS07910"
                     /old_locus_tag="pgaptmp_001582"
                     /EC_number="5.3.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019077755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008880 - glucuronate isomerase activity
                     [Evidence IEA];
                     GO_process: GO:0006064 - glucuronate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucuronate isomerase"
                     /protein_id="HGU4547547.1"
                     /translation="MTPFMTEDFLLDTEFARRLYHDYAKDQPIFDYHCHLPPQQIAED
                     YRFKNLYDIWLKGDHYKWRAMRTNGVAERLCTGDASDREKFDAWAATVPHTIGNPLYH
                     WTHLELRRPFGITGKLLSPSTADEIWNECNELLAQDNFSARGIMQQMNVKMVGTTDDP
                     IDSLEHHAEIAKDGSFTIKVLPSWRPDKAFNIEQATFNDYMAKLGEVSDTDIRRFADL
                     QTALTKRLDHFAAHGCKVSDHALDVVMFAEANEAELDSILARRLAGETLSEHEVAQFK
                     TAVLVFLGAEYARRGWVQQYHIGALRNNNLRQFKLLGPDVGFDSINDRPMAEELSKLL
                     SKQNEENLLPKTILYCLNPRDNEVLGTMIGNFQGEGMPGKMQFGSGWWFNDQKDGMER
                     QMTQLAQLGLLSRFVGMLTDSRSFLSYTRHEYFRRILCQMIGRWVEAGEAPADINLLG
                     EMVKNICFNNARDYFAIELN"
     gene            8374..9861
                     /gene="uxaA"
                     /locus_tag="ACHOSC_RS07915"
                     /old_locus_tag="pgaptmp_001583"
     CDS             8374..9861
                     /gene="uxaA"
                     /locus_tag="ACHOSC_RS07915"
                     /old_locus_tag="pgaptmp_001583"
                     /EC_number="4.2.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001199350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="altronate dehydratase"
                     /protein_id="HGU4547548.1"
                     /translation="MQYIKIHALDNVAVALADLAEGTEVSVDNQTVTLRQDVARGHKF
                     ALTDIAKGANVIKYGLPIGYALADIAAGEHVHAHNTRTNLSDLDQYRYQPDFQDLPAQ
                     AADREVQIYRRANGDVGVRNELWILPTVGCVNGIARQIQNRFLKETNNAEGTDGVFLF
                     SHTYGCSQLGDDHINTRTMLQNMVRHPNAGAVLVIGLGCENNQVAAFRETLGDIDPER
                     VHFMICQQQDDEIEAGIEHLHQLYNVMRNDKREPGKLSELKFGLECGGSDGLSGITAN
                     PMLGRFSDYVIANGGTTVLTEVPEMFGAEQLLMDHCRDEATFEKLVTMVNDFKQYFIA
                     HDQPIYENPSPGNKAGGITTLEDKSLGCTQKAGSSVVVDVLRYGERLKTPGLNLLSAP
                     GNDAVATSALAGAGCHMVLFSTGRGTPYGGFVPTVKIATNSELAAKKKHWIDFDAGQL
                     IHGKAMPQLLEEFIDTIVEFANGKQTCNERNDFRELAIFKSGVTL"
     gene            9944..10495
                     /gene="ygjV"
                     /locus_tag="ACHOSC_RS07920"
                     /old_locus_tag="pgaptmp_001584"
     CDS             9944..10495
                     /gene="ygjV"
                     /locus_tag="ACHOSC_RS07920"
                     /old_locus_tag="pgaptmp_001584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YgjV family protein"
                     /protein_id="HGU4547549.1"
                     /translation="MTAYWLAQGVGVIAFLIGITTFFNRDERRFKKQLSVYSAVIGVH
                     FFLLGTYPAGASAILNAIRTLITLRTRSLWVMAIFIVLTGGIGLAKFHHPVELLPVIG
                     TIVSTWALFRCKGLTMRCVMWFSTCCWVIHNFWAGSIGGTMIEGSFLLMNGLNIIRFW
                     RMQKRGIDPFKVEKTPPAVDERG"
     gene            complement(10500..11744)
                     /gene="sstT"
                     /locus_tag="ACHOSC_RS07925"
                     /old_locus_tag="pgaptmp_001585"
     CDS             complement(10500..11744)
                     /gene="sstT"
                     /locus_tag="ACHOSC_RS07925"
                     /old_locus_tag="pgaptmp_001585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015293 - symporter activity [Evidence
                     IEA];
                     GO_process: GO:0015826 - threonine transport [Evidence
                     IEA];
                     GO_process: GO:0032329 - serine transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine transporter SstT"
                     /protein_id="HGU4547550.1"
                     /translation="MTTQRSPGLFRRLAHGSLVKQILVGLVLGILLAWISKPAAEAVG
                     LLGTLFVGALKAVAPILVLMLVMASIANHQHGQKTNIRPILFLYLLGTFSAALAAVVF
                     SFAFPSTLHLSSSAGDISPPSGIVEVMRGLVMSMVSNPIDALLKGNYIGILVWAIGLG
                     FALRHGNETTKNLVNDMSNAVTFMVKLVIRFAPIGIFGLVSSTLATTGFSTLWGYAQL
                     LVVLVGCMLLVALVVNPLLVWWKIRRNPFPLVLLCLRESGVYAFFTRSSAANIPVNMA
                     LCEKLNLDRDTYSVSIPLGATINMAGAAITITVLTLAAVNTLGIPVDLPTALLLSVVA
                     SLCACGASGVAGGSLLLIPLACNMFGISNDIAMQVVAVGFIIGVLQDSCETALNSSTD
                     VLFTAAACQAEDDRLANSALRN"
     gene            complement(12143..13108)
                     /gene="alx"
                     /locus_tag="ACHOSC_RS07930"
                     /old_locus_tag="pgaptmp_001586"
     CDS             complement(12143..13108)
                     /gene="alx"
                     /locus_tag="ACHOSC_RS07930"
                     /old_locus_tag="pgaptmp_001586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TerC family membrane protein Alx"
                     /protein_id="HGU4547551.1"
                     /translation="MNTVGTPLLWGGFAVVVAIMLAIDLLLQGRRGAHAMTMKQAAAW
                     SLVWVTLSLLFNAAFWWYLVQTEGRAVADPQALAFLTGYLIEKSLAVDNVFVWLMLFS
                     YFSVPAALQRRVLVYGVLGAIVLRTIMIFTGSWLISQFDWILYIFGAFLLFTGVKMAL
                     AHEDESGIGDKPLVRWLRGHLRMTDTIDNEHFFVRKNGLLYATPLMLVLILVELSDVI
                     FAVDSIPAIFAVTTDPFIVLTSNLFAILGLRAMYFLLAGVAERFSMLKYGLAVILVFI
                     GIKMLIVDFYHIPIAVSLGVVFGILVMTFIINAWVNYRHDKQRGG"
     gene            complement(13391..14377)
                     /gene="ygjR"
                     /locus_tag="ACHOSC_RS07935"
                     /old_locus_tag="pgaptmp_001587"
     CDS             complement(13391..14377)
                     /gene="ygjR"
                     /locus_tag="ACHOSC_RS07935"
                     /old_locus_tag="pgaptmp_001587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417558.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HGU4547552.1"
                     /translation="MIRFAVIGTNWITRQFVEAAHESGKYKLTAVYSRSLEQAQHFAN
                     DFSVEHLFTSLEAMAESDAIDAVYIASPNALHFSQTQLFLSHKIHVICEKPLASNLAE
                     VDAAIACARENQVVLFEAFKTACLPNFHLLRQALPKVGKLRKVFFNYCQYSSRYQRYL
                     DGENPNTFNPSFSNGSIMDIGFYCLASAVALFGEPKSVQATASLLASGVDAHGVVVMD
                     YGDFSVTLQHSKVSDSVLASEIQGEAGSLVIEKLSECQKVCFVPRGSQMQDLTQPQHI
                     NTMLYEAELFATLVDEHLVDHPGLAVSRITAKLLTEIRRQTGVIFPADSVKL"
     gene            complement(14456..15135)
                     /gene="ygjQ"
                     /locus_tag="ACHOSC_RS07940"
                     /old_locus_tag="pgaptmp_001588"
                     /pseudo
     CDS             complement(14456..15135)
                     /gene="ygjQ"
                     /locus_tag="ACHOSC_RS07940"
                     /old_locus_tag="pgaptmp_001588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001183047.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="vancomycin high temperature exclusion protein"
     gene            complement(15212..15715)
                     /gene="ygjP"
                     /locus_tag="ACHOSC_RS07945"
                     /old_locus_tag="pgaptmp_001589"
     CDS             complement(15212..15715)
                     /gene="ygjP"
                     /locus_tag="ACHOSC_RS07945"
                     /old_locus_tag="pgaptmp_001589"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311994.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family metallopeptidase"
                     /protein_id="HGU4547553.1"
                     /translation="MSNLTYLQGYPEQLLSQVRTLINEQRLGDVLAKRYPGTHDYATD
                     KALWQYTPDLKNQFLRNAPPINKVMYDNKIHVLKNALGLHTAVSRVQGGKLKAKAEIR
                     VATVFRNAPEPFLRMIVVHELAHLKEKEHNKAFYQLCCHMEPQYHQLEFDTRLWLTQL
                     SLGQDKI"
     gene            15800..16936
                     /gene="rlmG"
                     /locus_tag="ACHOSC_RS07950"
                     /old_locus_tag="pgaptmp_001590"
     CDS             15800..16936
                     /gene="rlmG"
                     /locus_tag="ACHOSC_RS07950"
                     /old_locus_tag="pgaptmp_001590"
                     /EC_number="2.1.1.174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0008990 - rRNA
                     (guanine-N2-)-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (guanine(1835)-N(2))-methyltransferase
                     RlmG"
                     /protein_id="HGU4547554.1"
                     /translation="MSHLDNGFRSLTLQRFPATDDVNPLQAWEAADEYLLQQLDDTEI
                     RGPVLILNDAFGALSCALAEHKPYSISDSYISELATRENLRLNGIDESSVKFLDSTAD
                     YPQQPGVVLIKVPKTLALLEQQLRALRKVVTSDTRIIAGAKARDIHTSTLELFEKVLG
                     PTTTTLAWKKARLINCTFNEPPLADAPQTVSWKLEGTDWTIHNHANVFSRTGLDIGAR
                     FFMQHLPENLEGEIVDLGCGNGVIGLTLLDKNPQAKVVFVDESPMAVASSRLNVETNM
                     PEALDRSEFMINNALSGVEPFRFNAVLCNPPFHQQHALTDNVAWEMFHHARRCLKING
                     ELYIVANRHLDYFHKLKKIFGNCTTIATNNKFVVLKAVKLGRRR"
     gene            17127..17210
                     /locus_tag="ACHOSC_RS07955"
                     /old_locus_tag="pgaptmp_001591"
                     /pseudo
     CDS             17127..17210
                     /locus_tag="ACHOSC_RS07955"
                     /old_locus_tag="pgaptmp_001591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311992.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HigB family
                     toxin"
     gene            17207..17623
                     /gene="higA"
                     /locus_tag="ACHOSC_RS07960"
                     /old_locus_tag="pgaptmp_001592"
     CDS             17207..17623
                     /gene="higA"
                     /locus_tag="ACHOSC_RS07960"
                     /old_locus_tag="pgaptmp_001592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system antitoxin HigA"
                     /protein_id="HGU4547555.1"
                     /translation="MIAIADILQAGEKLTAVAPFLAGIQNEEQYTQALELVDHLLLND
                     PENPLLDLVCAKITAWEESAPEFAEFNAMAQAMPGGIAVIRTLMDQYGLTLSDLPEIG
                     SKSMVSRVLSGKRKLTLEHAKKLATRFGISPALFID"
     gene            complement(17668..19686)
                     /gene="fadH"
                     /locus_tag="ACHOSC_RS07965"
                     /old_locus_tag="pgaptmp_001593"
     CDS             complement(17668..19686)
                     /gene="fadH"
                     /locus_tag="ACHOSC_RS07965"
                     /old_locus_tag="pgaptmp_001593"
                     /EC_number="1.3.1.34"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent 2,4-dienoyl-CoA reductase"
                     /protein_id="HGU4547556.1"
                     /translation="MSYPLLFAPLDLGFTTLKNRVLMGSMHTGLEEYPDGAERLAAFY
                     AERARHGVALIVSGGIAPDLTGVGMEGGAMLNDASQIPHHRTITEAVHQEGGKIALQI
                     LHTGRYSYQPHLVASSALQAPINRFVPHELTHEEILQLIDDFAHCAQLAREAGYDGVE
                     VMGSEGYLINEFLTLRTNQRSDQWGGDYRNRMRFAVEVVRAVRERVGNDFIIIYRLSM
                     LDLVEDGGTFAETVELAQAIEAAGATIINTGIGWHEARIPTIATPVPRGAFTWVTRKL
                     KGHVSLPLVTTNRINDPQVADDILSRGDADMVSMARPFLADAELLSKAQSGRADEINT
                     CIGCNQACLDQIFVGKVTSCLVNPRACHETKMPILPAVQKKNLAVVGAGPAGLAFAIN
                     AAARGHHVTLFDAHSEIGGQFNIAKQIPGKEEFYETLRYYHRMIEVTGVTLKLNHTVT
                     ADQLQAFDETILASGIVPRVPPIDGIDHPKVLSYLDVLRDKAPVGNKVAIIGCGGIGF
                     DTAMYLSQPGESTSQNIARFCNEWGIDSSLQQAGGLSPQGMQIPRSPRQIVMLQRKAS
                     KPGQGLGKTTGWIHRTTLLSRGVKMIPGVSYQKIDDDGLHVVINGETQVLAVDNVVIC
                     AGQEPNRALAQPLIDSGKTVHLIGGCDVAMELDARRAIAQGTRLALEI"
     gene            complement(20012..22363)
                     /gene="ygjK"
                     /locus_tag="ACHOSC_RS07970"
                     /old_locus_tag="pgaptmp_001594"
                     /pseudo
     CDS             complement(20012..22363)
                     /gene="ygjK"
                     /locus_tag="ACHOSC_RS07970"
                     /old_locus_tag="pgaptmp_001594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417551.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004555 - alpha,alpha-trehalase activity
                     [Evidence IEA];
                     GO_process: GO:0005991 - trehalose metabolic process
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glucosidase"
     gene            complement(22380..>23439)
                     /gene="ygjJ"
                     /locus_tag="ACHOSC_RS07975"
                     /old_locus_tag="pgaptmp_001595"
     CDS             complement(22380..>23439)
                     /gene="ygjJ"
                     /locus_tag="ACHOSC_RS07975"
                     /old_locus_tag="pgaptmp_001595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="protein YgjJ"
                     /protein_id="HGU4547557.1"
                     /translation="TAPCRALLALPFCYAFSAAGEEARPAEHDDTKTPAITSTSSPSF
                     RFYGELGVGGYMDLEGENKHKYSDGTYIEGGLEMKYGSWFGLIYGEGWTVQADHDGNA
                     WVPDHSWGGFEGGINRFYGGYRTNDGTEIMLSLRQDSSLDDLQWWGDFTPDLGYVIPN
                     TRDIMTALKVQNLSGNFRYSVTATPAGHHDESKAWLHFGKYDRYDDKYTYPAMMNGYI
                     QYDLAEGITWMNGLEITDGTGQLYLTGLLTPNFAARAWHHTGRADGLDVPGSESGMMV
                     SAMYEALKGVYLSTAYTYAKHRPDHADDETTSFMQFGIWYEYGGGRFATAFDSRFYMK
                     NASHDPSDQIFLMQYFYW"
BASE COUNT         5879 a         5981 c         6064 g         5515 t
ORIGIN      
        1 atctcttatc acggcttacg ccgcttttat tcgtgttcgc tccttttttg gcacagtcaa
       61 atatgactgt ttatcctatg gctgtttcca ttaatagtca gggtgaaggc aacgttcgcg
      121 taatatctaa aagtaatgaa gttcagtaca tcaaggcgac agtattccgt atcgataatc
      181 catcgacgcc tcaggaaaat gaagttgaga ttaaatccgg tgatgcaaat catctggttg
      241 ttatgccacc taaattcgct ttaccagccg gtagtagcaa aaccgtacgt tttgttgcga
      301 tggaaccaga gcaaaaagag aaaaattatc gcgttaaatt tgaagcggtt cccagtattg
      361 atgacgttgc cacagataaa aaagatctct ctatgcagtt aacagttaac ttaatttggg
      421 ggattgttgt tagtgttcca cctcagcaac ctattgctaa gttagaagta aatgctgccc
      481 aaaaattagt taatgcaggg aatcaacgct taaagatttt aacgatcgct tattgtaaaa
      541 ataatagcaa agaaaattgt aagatacaga ccgtaaataa aaacatcttc cctggtcagg
      601 aaagaaatct tgaaagcata tctggctacg acaaaatcgt tgtcaaatat aataactgga
      661 tcaccaaaga taatggcgag tttgaactgg cggtccatta attatcattg taataaacga
      721 aggaatagta atgaaaaagg tatttgcaaa atctcttctg gtcgcagcga tgttttctgt
      781 tgcaggctcc gcattggcag tacaaaagga tattactgta acggccaacg ttgatgccgc
      841 tctggatatg acacagaccg ataacaccgc gttgccgaaa gcagtagaaa tgcaatatct
      901 gccggggcag ggtctgcaat cttaccagtt gatgaccaaa atctggtcta acgacgttac
      961 caaagatgta aaaatgcagt tagtttcccc ggcgcaactg gttcaaagcc tggatgctag
     1021 caagattgtt ccgctgacag taacctgggg cggtgaagaa attaaagctg atgcggcaac
     1081 cacttttacc gcaaccaaaa tctttgcttc cgatgcgtta actaacggtt ctctggctaa
     1141 aaacctgatg tttgctcaaa tcaccaaagg tgttctggag acaggcatct accgtggtgt
     1201 agtaagcatt tatctgtctc aggatatcta atccagataa cagaattaac acgtaacaaa
     1261 gggaggggta gccctccctt attttaactg ttttagtatt tgttatggat ttcaggattg
     1321 ccatggataa aaaattactg gctcttttga tcctggcgag tctcagcccg gcagaggcga
     1381 cattaaccaa aattcccgca gggtttgagg ttattgctca gggacagcag gagtatatcg
     1441 aggtttattt ttcagggaaa agtctcggta aatattatgc aatggttaat cttgataccg
     1501 taacatttct tgatccagca agtctatata acaagctgga actggatgta gacgatcaga
     1561 aaatcgcgca tatagtgaaa gaaaaattat cgcagccgct agctcgccac ggtgaattgg
     1621 cttgcggtta tgtacgtact gactcagggt gtggttttct gaataccgat acgctggaaa
     1681 taatctataa tgatgaagaa agttcggcaa cgttgtttat taatccgcaa tggaattcag
     1741 ctttcgatgc gaagtcatta tatttaaatc cagacaaaaa tactgttaat gcttttatac
     1801 atcagcaaga catcaatgtt ctggcacagg atgattacca atcgttgtct attcagggaa
     1861 acggtgcgct gggaataaca gaaaatagtt atattggtgc acactggaat ttcaacggtt
     1921 atgatgcaga tgatgtcagt gacagtaatg ctgacatcag cgatctctat tatcgttatg
     1981 attttttacg tcgttattat gtgcaggcgg gtcgcatgga caaccgcaca ctatttaatg
     2041 cacaaggcgg gaactttacc tttaactttc tgccactcgg tgcaattgat gggatgcgta
     2101 tcgggtcgac actgagctat ttaaaccagg cgcaaagcca gcaaggaacc ccagtaatgg
     2161 ttctgctttc gcgcaattct cgtgttgacg cttatcgtaa tgagcaactt ttgggatcgt
     2221 tttatctcaa tagtggttcg caatttattg ataccagttc ctttccgcca ggcagctaca
     2281 gcgtggcatt aaaagtctac gaaaataacc aactcacccg caccgagctt gtgccgttta
     2341 ccaaaaccgg cggtctgact gacggaaatg cgcaatggtt cttacaggca ggtaaaacta
     2401 catcacaggt ttctgatgat gaaagttcag cttatcagct aggagtacgc ctgccattac
     2461 atccgcaata tgagctctac gcagggctgg cgaatgccga tgatgtgagt gctttcgagt
     2521 taggtaataa ctggacggca gatttaggcg gggtggggaa tcttgcaatc agcgccagcg
     2581 tgttccgtaa cgatgacggc ggcaaaggtg atatgcaaca ggccaactgg agtaatccgg
     2641 gatggccgac gttgggcttt tatcggacca actctgacgg cgatgcttgt acaaccgaca
     2701 gcagagagag ctataacgcc ttaagctgtt atgaaagtat ttccgcgacg gtttcacaga
     2761 attttgtcgg ctggaatatg atgctgggtt atacccgcac acaaaataac actgatgata
     2821 gtttgcgttg ggataaacag cagagctttg aaaataacta tcttcgccag acaacagcgc
     2881 aaagtatctc tgaaactgta caacttagcg cttcccgcgc ttttgtgatg cgtgactgga
     2941 ttttgagtac gtcagtcggt gttttccatc gtaatgacaa cggtggcgat aacgacgaca
     3001 acggcttgta cttatcgttt tcgttatctg acacgccaac gatggatagc aataacaaca
     3061 gccattcaac aaatgtgtct acggattatc gttatagcga acaggatggc gatcaaacgt
     3121 catggcagtt atcgcatacc ttttataacg attcattcag ccataaagaa cttggcgtaa
     3181 ccgtcggagg cctgaacacc gataccataa acagcgcggt taacgggcgt tgggatgggc
     3241 aatacggaaa tgtctacgct accgtatctg atagttatga ccgtaagaat catgatcatc
     3301 tttcggcctt tacgggcact tatagctcta cgctggctgt cagtcgctat ggcgttaatt
     3361 tgggagccag tggtacagac ggtttgctgg gtgcggtact ggtggatgtg aaaggcttct
     3421 ctgaacagga tgaagagagt caggatctgc aactcgaagc gcgggtggca ggcagccgaa
     3481 ctttgcagct tggtcaaagt gacagtgtgt tgttccctta tcctggattt cagtctggtt
     3541 ttgttgaggt taacgacagt agccagggca atcagcaggg gacaacaaac atcattaacg
     3601 gtgcagggaa tcgtgaatta atgttgttgc ctggcaagct gcgctatcgc gaagtgtctg
     3661 ccagctttaa ctacaactat atcggtcgtt tattactgcc ggcagcggtg aaaaaattcc
     3721 cgattgtggg gctgaacagc gccatgttac tggtagctga agatggcgga tttacacttg
     3781 aaattaacgg cagcgaaaaa gagctctatc tgctttccgg gcagcaattc cttaagtgtc
     3841 cactgagtgt tgtaaagaaa cgcgccagca ttcgttacag cggagatgtt acttgtagtg
     3901 tggtgactta ttcacaatta ccggagtcga ttcaggttca ggcacagttg aaacagccta
     3961 aattacgtgg aaacgttcag acggcgcaaa gggaggttgc accatgagaa atcgattgat
     4021 tgcggcgata ttgggcttgt ttggttcagg cagctcctga cgtgaccagt gaaattacgt
     4081 atgatttggc atctggtaga gcggattatt acttctggaa ggatgaagcg tcggcaggaa
     4141 ataatggata tatgtggtat gaatgttcgt atcctgacct ccaacaaacc tgtacagcta
     4201 atggaaatat atcgacagta caaatctatt taactgaaca acgcagtggg atgcgttggc
     4261 cggtaaaact caaaggattt aaaacagcca ttgtaagtag cgatgaagcg ccgccaggat
     4321 gcaagggggg caaagggctt cagacgaatc ttaaggattc taatagatct tcatgtacag
     4381 aagatggtca acattattat atatacgata caaagtttct tacgctctac cttgagcaga
     4441 cagagatgaa gaatttgccg attggtggcg tctggaaggg gaaagttaaa ttacattcga
     4501 acagcccggc ccaggactat ttcgcaaata ttaccctgaa tacgctcgac cccaaccata
     4561 ttgacgtgtt cttcccggag ttcgcccacg ccacgccaag ggtgcagtta gacttgcatc
     4621 caacaggaag cgttaacggc agcaactacg cgcaagatct gaccatgttg gacatgtgcc
     4681 tgtacgatgg ttttaacggt aatgccatca gttatgaaat catgctcaaa gatgaagggc
     4741 gaccagctgc agggcgcaga gacggttact tctctatcta tcgtcaggga gggaccacca
     4801 ccgacgaggg agaacgcatt gattaccggg tcaaaatgta caacccggaa accggtgggc
     4861 aaattgatgt gcgcaataat gaaaatatgg tctggaacag cattaacctg aaacgtgtgc
     4921 gtccggtggt actgcccggt attcgctatg ccgtgatgtg tgtgccgaca cccttgactc
     4981 tggcagtcga taaattcagc gtgatggata aacaggccgg gtactacatg ggcaaattgt
     5041 cagtaatctt tacgccttcc ttgccaacca tcaattaacc ctgcaaaccg gacaaacgct
     5101 tttttctata taaacgacga agccgtctga taaagcggct tcgtcattac aacttaagtt
     5161 aaacgttaat gttgcggtgc gggatcgtta tgtgtttcct gcgccacctc aatcgccggt
     5221 ttgttctgca acacggtcca gataaccagc gcacctaaca ggtcgaacac tgccagaact
     5281 gcgaacagcg ggctgaagcc gatggtgtca gccagtgcac cgacaaccag cgcaaacagc
     5341 gtacttgcca gccatgcgga catcccggtt aaaccgttcg ccgtcgccac ttcgttacga
     5401 ccgaacacgt cagaagagag cgtaatcagc gcgccagaca gtgcctggtg ggcaaaacca
     5461 ccgatacaca gcagcataat tgcgacatac gggttggtga acagaccgat catacccggg
     5521 ccaatcatca gcacagcacc cagcgttacg accatcttac gggaaacgat caggttcaca
     5581 ccaaaccaac gctggaacag cggcggcagg taaccaccga ggatacaacc gaggtcagca
     5641 aacagcatcg gcatccaggc gaacatcgcg atctctttca ggttaaagcc gtaaacttta
     5701 aacatgaaca gcgggatcca cgcgttaaaa gtaccccagg ccggttctgc caggaaacgc
     5761 ggcagcgcga taccccagaa ctgacggtta cgcaggatct gaccaacgga cattttcttc
     5821 gccgtgctca cctggtgctg ggcttcctga ccattaataa tatagtcgcg ttcttcatcg
     5881 gtcagatgct tctggtcgcg cggatgttta tagaaaatca gccatgccat cgcccagata
     5941 aagctcaatg caccggagat gataaatgcc atctgccagc tgtgcattac gattgcccat
     6001 accaccagcg gcggcgcaat catcgcacca atcgaagaac ctacgttaaa gtaacctacc
     6061 gcgatggaac gctctttcgc cgggaaccat tcggagctgg ctttcagacc cgccgggatc
     6121 atcgctgctt ccgcggcacc gactgcacca cgagcaatcg ccaggccacc ccagctacca
     6181 gccagcgcgg ttgcaccaca gaacacggcc cacagcacag caaacattgc ataaccgatt
     6241 ttcgtaccca gcacatccag tacataacct gctaccggtt gcatgaccgt ataagcagca
     6301 gaataggctg cgatgatata ggaatactgt tgggtggaga tgtttaactc ttccatcaga
     6361 gttggcgcag ctgccgccac agtgttacgc gtcaggtaac caagcacggt gccgagcgtc
     6421 accagtgcga tcatatacca acgtaaccct ttaattttac gcatgtaaaa cctcatcgtt
     6481 ttgttatatc cgcaggaagc gcggccatca tcccgccaga ggctgaagat gttagttacg
     6541 accgggtaac cgaaaaaaaa ccggcacgcc ccgaacgttg ccatgaaagt ttcgtgctaa
     6601 ttcggcttcc gtaccggtta acccgattca tctaacaaat gaatgaatcg gcaatttgca
     6661 ttccgtcgac ttatgatttg cgacggcaga aagataactt gtcatacaac tttaaaaggt
     6721 gagagccatc acaaatgtgc gaatatttgt agggacatta cctgacgaca gcgaggccag
     6781 tactggcgcg gcctgcagag agatttacca ctttgagagt aattttttta actacgttta
     6841 ttgatctaac tcacgaaaat atcttcggac tctggaaatt ggtgtgataa ctttgtcagc
     6901 atcgcaccat aagcaagcta gctcactcgt tgagaggaag acgaaaatga ctccgtttat
     6961 gactgaagat ttcctgttag ataccgaatt tgcccgccgt ctgtatcacg actacgcaaa
     7021 agaccagccg attttcgatt accattgcca tttgccgccg cagcagattg cggaagacta
     7081 tcgttttaaa aacctgtatg acatctggct gaaaggcgat cactacaaat ggcgcgctat
     7141 gcgtaccaac ggtgtggccg agcgtctgtg taccggtgat gcgtctgacc gtgaaaaatt
     7201 tgacgcctgg gcggcgactg ttccgcacac tatcggcaac ccgttatacc actggacgca
     7261 cctcgaactg cgtcgtccgt ttggtatcac tggcaaattg ctttctccgt caactgccga
     7321 tgaaatctgg aacgaatgta acgaactgct ggcgcaggat aacttctccg cgcgcggcat
     7381 catgcagcag atgaacgtga aaatggtcgg caccaccgat gacccgatcg attctctgga
     7441 gcatcacgca gagatcgcca aagacggctc tttcaccatc aaagtgctgc cgagctggcg
     7501 tccggacaaa gccttcaaca tcgaacaggc gacctttaac gactacatgg cgaagctggg
     7561 cgaagtttcc gataccgaca ttcgccgctt tgctgacctg caaactgcct tgaccaaacg
     7621 tctggatcac ttcgccgctc acggctgtaa agtgtctgac catgcgctgg acgtggtgat
     7681 gtttgctgaa gcgaacgaag cggaactgga cagcattctg gcgcgccgtc tggctggcga
     7741 aaccctgagc gagcacgaag tggcacagtt caaaactgcg gtactggtgt tccttggtgc
     7801 cgaatatgca cgtcgcggct gggtacagca gtaccacatt ggcgcgctgc gtaataacaa
     7861 cctgcgtcag ttcaaactgc tggggccgga tgtaggcttt gactccatca acgaccgtcc
     7921 gatggcagaa gagttgtcta agctgctgag caagcagaac gaagaaaacc tgctgccgaa
     7981 aaccattctg tactgcctga acccgcgcga taacgaagtg ctgggcacca tgatcggtaa
     8041 cttccagggc gaaggtatgc cgggcaaaat gcagttcggt tccggctggt ggtttaacga
     8101 tcagaaagac ggtatggaac gtcagatgac ccaactggcg cagctcggtt tgctgagccg
     8161 ctttgtcggt atgctgactg acagccgtag cttcctgtca tacacccgtc acgaatactt
     8221 ccgccgcatt ctgtgccaga tgatcggtcg ctgggtggaa gcgggcgaag caccggcgga
     8281 catcaacctg ctgggcgaga tggtgaaaaa tatttgcttt aacaatgcgc gtgactactt
     8341 cgccattgaa ctgaactaag gtctggggtt gatatgcaat acatcaagat ccatgcgctg
     8401 gataacgtcg cggtcgcttt agcggatttg gctgaaggca cagaagtcag tgtcgataac
     8461 cagactgtta cgctgcgcca ggatgttgct cgtggacata aatttgcgtt aacggatatc
     8521 gcaaaagggg ccaatgtcat caaatatggc ctgccgattg gttatgcatt ggcggatatt
     8581 gcggcggggg aacacgtcca cgcccacaat acgcgcacga atctgagcga tctggatcag
     8641 tatcgctatc aacctgattt tcaggatctg cctgcgcaag cggcagatcg tgaagtgcag
     8701 atctatcgtc gcgctaacgg cgatgtcggg gtgcgtaacg agctgtggat cctgccaacc
     8761 gtgggctgtg tcaacggcat cgcgcggcaa atccagaacc gtttcctgaa agagaccaac
     8821 aacgccgaag gtaccgacgg cgtgttcctc ttcagccaca cctacggctg ctcacaactg
     8881 ggcgacgatc acatcaacac ccgcaccatg ctgcaaaaca tggtgcgcca cccgaacgcg
     8941 ggcgcagtgc tggtgattgg tctgggctgt gaaaacaacc aggttgccgc attccgtgaa
     9001 acgttgggcg atatcgatcc tgaacgcgtt catttcatga tctgccaaca gcaggatgat
     9061 gagatcgaag ccgggatcga gcatttgcat cagctgtata acgtgatgcg caacgataaa
     9121 cgcgagccag gcaaactcag cgaactgaag tttggtctgg agtgcggtgg ttctgacggt
     9181 ctttctggta ttactgctaa cccgatgctg gggcgtttct ctgactacgt gattgctaac
     9241 ggcggtacta ccgtactgac cgaagtgccg gagatgtttg gcgcagagca gttgctgatg
     9301 gaccattgcc gcgacgaagc aacgtttgaa aaactggtga ccatggtcaa cgacttcaaa
     9361 cagtacttta ttgcccatga ccagccgatc tatgaaaacc catcgccggg gaacaaagcg
     9421 ggcggtatca ccacgctgga agataaatcc cttggctgta cccagaaagc gggttccagc
     9481 gtcgtggttg acgtgctgcg ttacggcgag cgtctgaaaa cgccggggct gaacttgtta
     9541 agtgcgccgg gtaacgatgc cgtagcgacc agcgccctgg cgggtgcggg ctgccatatg
     9601 gtgctgttca gtactggtcg tggcacgcca tacggcggct ttgtaccgac ggtgaaaatc
     9661 gctaccaaca gtgaactggc ggcgaagaaa aaacactgga tcgactttga cgcgggtcag
     9721 ctgatccacg gtaaagcgat gccgcagttg ctggaagaat ttatcgacac catcgttgag
     9781 tttgccaacg gtaaacaaac ctgcaacgag cgtaacgact tccgcgaact ggcgatcttc
     9841 aaaagcggcg taacgctata aagtcgaaat gagttgttaa taaaaaacgg cgtttcataa
     9901 tgtgaagcgc cgtttgcttt tccatacctg ataaggattt atcatgaccg cgtattggct
     9961 ggcccagggc gtgggtgtca tcgcctttct gattggtatc acaacatttt tcaatcgtga
    10021 cgaacgtcgc ttcaaaaagc agctttcggt ctatagcgcc gttattggcg tacatttttt
    10081 tcttctgggc acctatcccg ctggtgccag tgccatcctt aatgccattc gtacattgat
    10141 taccttacgc acgcgcagct tatgggtaat ggcgattttt attgtgctga ctggcggaat
    10201 tggcctcgcg aagttccatc atcctgtcga actattgccg gttatcggta cgattgtcag
    10261 tacctgggcg ctgttccgct gtaaagggct caccatgcgc tgcgtgatgt ggttttcaac
    10321 gtgttgctgg gtgattcaca acttctgggc ggggtcgata ggcggcacaa tgattgaggg
    10381 tagttttctg cttatgaatg gcttgaatat cattcgtttc tggcggatgc agaaaagggg
    10441 aattgatccg tttaaagtag agaaaacccc tcccgccgta gacgaaaggg gttaaacaat
    10501 taattacgca gggcgctatt tgccagacga tcgtcttccg cctggcaagc tgccgcagtg
    10561 aacagcacgt cagttgaaga gttcagcgcg gtttcgcaag agtcctgcaa tacgccgatg
    10621 ataaagccga cggcaaccac ctgcatggcg atatcgttcg aaataccgaa catattacag
    10681 gccagcggga tcagcagcag agaacccccc gccacgccgg atgcgccaca ggcacacaga
    10741 gaagccacca cgctcaacag cagcgccgtg ggcagatcga ccgggatacc cagcgtatta
    10801 accgcagcca gcgtcaacac ggtaatggtg attgctgcgc ccgccatatt gatggtggca
    10861 cccagcggaa tagaaacgga ataggtatcg cgatccagat tcagcttttc acacagtgcc
    10921 atattcaccg gaatgttcgc cgcagagctg cgggtgaaga aggcatacac gccgctttcg
    10981 cgcaggcaca gcagcaccag cgggaacggg ttacgacgaa ttttccacca caccagcaat
    11041 gggttaacca ccagcgccac cagtaacata cagccaacca gcacgaccag cagttgcgcg
    11101 tagccccaca gtgtggagaa accggtggtt gccagggtag aagaaaccag accaaaaata
    11161 ccaatcggtg cgaagcgaat gaccagtttc accataaagg tgacggcatt cgacatatcg
    11221 ttgaccaggt ttttggtggt ctcgttaccg tgacgcagtg cgaaaccgag gccaattgcc
    11281 cacaccagga tcccgatgta gttacctttc agcagcgcgt cgatggggtt ggaaaccatg
    11341 ctcattacca gcccgcgcat cacttctaca atgcctgacg gtggcgaaat atcacccgcg
    11401 ctactggata agtgcagggt agaagggaag gcgaagctga aaacgactgc ggccagagca
    11461 gcagagaagg tacccagcag atagaggaac aaaatagggc ggatattggt tttctgcccg
    11521 tgctggtggt tagcaataga tgccatcacc agcatcaaca ccaggatggg ggcaacggct
    11581 ttcagtgcgc cgacgaacaa agtacctaac agaccaacag cttccgccgc gggttttgag
    11641 atccatgcca gaagaatccc cagaacaagg ccgaccagga tttgttttac caggctgcca
    11701 tgagccagac gccggaatag ccccggtgaa cgttgcgtag tcatttttcg atcctttcat
    11761 tgtgttgtcg cacatccctg gtgcgttctg tcgacgcatc tttcaggatg taacaaagtg
    11821 tttgcaaggt cgagtataag gaaagttaca gcttcgggaa gcgaaaaatg ctggatttta
    11881 aagagaggtc atattttgca acttaatggt tacatttatt tgacataaaa ataaagccaa
    11941 cgtgcggctt ccagactgat gacaacatca aaaaaggact cggtcagtcc cctcgccccg
    12001 tttggggaga gggttagggt gaggggaaca ggccagcatc ggtgcgaaaa attaccctca
    12061 ccccgaccct ctccctggaa gggagaggga gaaaacggtg ctcatgttaa tgacagggta
    12121 cacggcttag gcagattaaa aattatccac cccgctgctt atcatgccga taattcaccc
    12181 aggcgttgat aataaacgtc atcaccagaa tgccaaacac cacgcccagc gagacggcga
    12241 ttggaatatg gtagaagtcg acaatcagca tcttgatacc gataaacacc agaatcaccg
    12301 ccaggccata tttgagcatc gagaaacgct ctgctacgcc cgccagcagg aaatacatcg
    12361 cacgcaggcc gaggattgca aacaggtttg aggtcagcac aataaacgga tcagtggtca
    12421 cggcgaagat agccggaatg ctatccacgg caaaaatcac gtcgctcaat tccaccagaa
    12481 tcagtaccag catcagcggt gtggcgtaca gcaatccatt cttacgcaca aagaaatgct
    12541 cgttgtcgat ggtgtcggtc atgcgcaaat gaccgcgtag ccagcgcacc agcggcttgt
    12601 cgccaatgcc tgattcatct tcatgggcga gcgccatctt cacgccggta aacagcagga
    12661 aggcaccgaa gatatacagg atccagtcga actgtgaaat cagccagctg cctgtgaaga
    12721 tcatgatggt acgcagaaca atcgccccga gcacgccata caccagcacg cggcgttgta
    12781 atgccgccgg aacagagaaa tagctgaaca acatcagcca gacaaagacg ttatcgaccg
    12841 ccagcgattt ctcaatcaga tagcccgtga gaaaggccag tgcctgtgga tcggcaacgg
    12901 cgcgaccttc agtttgcacc agataccacc agaaagcggc gttaaacagt aacgacagcg
    12961 tcacccagac cagcgaccag gccgcagcct gtttcatggt catggcatgt gccccacgac
    13021 gcccctgcaa caacaggtcg atagccagca taatggcgac aacaacagcg aatccgcccc
    13081 atagcaacgg cgtgccgaca gtattcatag aagttcctta cacataaaaa aacggccaac
    13141 gtcggaagac gtcagccgct gctttttatg catagacctc gccttccggc aaggtctcac
    13201 ttacaacaaa aaaggaatac gtctgcgtat tcctttcggg ttgcccggtg accggatgtg
    13261 gtttttcaca catcgtaatg acgatcgacc ggcaatgaag ttactcccct ttgcaggtaa
    13321 caaagtatga cagaccattc agccagtcaa tggtctgtta catacctttt acactgtttt
    13381 actttggcaa ttatagtttt acgctatctg ccggaaaaat caccccagtc tggcggcgga
    13441 tctcggtcag cagtttggcg gtgatgcgac tgaccgccag ccccggatga tccaccagat
    13501 gctcatccac cagggtagcg aacagctctg cttcgtagag catggtatta atatgctgcg
    13561 gctgggtgag atcctgcatt tggctgccac gcggcacgaa gcacactttc tggcattcag
    13621 acagtttttc aatcaccagc gatcctgctt cgccctgaat ctcgctcgcc aggacagaat
    13681 cactgacttt tgagtgctgc aaggtgacgc tgaaatcacc gtaatccatc accaccacgc
    13741 cgtgggcatc tacaccgctt gccagcaaac tggcggttgc ctgcacgctt ttcggctcac
    13801 caaataacgc caccgccgac gccaggcagt aaaagccgat atccataatt gaaccgttag
    13861 agaatgacgg attaaaggtg ttgggatttt caccgtccag gtaacgttga tagcgcgagg
    13921 aatactggca atagttgaaa aagactttac gcaatttgcc gactttcggc agcgcctggc
    13981 gcagcaaatg aaagttcggc aggcaggcgg ttttaaatgc ctcaaacagc accacctgat
    14041 tttcccgcgc acaggcaatg gcggcatcca cttccgccag attcgacgcc agtggtttct
    14101 cgcaaatcac atgaatttta tggctaagga aaagttgtgt ctgggaaaag tgcagggcat
    14161 tcgggctggc aatatacacc gcgtcaatgg catcgctttc cgccatcgct tccagcgagg
    14221 taaacagatg ctcgacagaa aaatcattgg cgaagtgctg agcctgctca aggctgcggg
    14281 aatatacggc ggttaacttg tatttaccgc tctcatgggc ggcctcgacg aactggcgag
    14341 tgatccagtt cgtaccaatc acagcgaaac gtatcataac gcgtggatac tccataaagg
    14401 gattctgtcg tcaatgtagc acgccttttt catcttcgac gtgcgcagat acgcattatt
    14461 cttttgccgg acagccatgc tcactaaacg gcccaatcat gaccgatggc ccgagatatt
    14521 tcggctggcg atggaggatt ttggcgtcaa tcacggcgct gacacgagcg agtttctccc
    14581 gcagttgagt ataaaaaccg tgcttcatat tgaggtcagg cgcgttaaaa ccgatagcgt
    14641 cgatgccgta ctgttttgcc agccagatgg cgcgttgatt atgaaattct tgcgagataa
    14701 tggtgatatg gttttcacca aaaacctttt tggctcgcac taccgaatcc agcgttgaga
    14761 atccggcata gtcgcagaag atcactttcg ccggtacgcc tttagcgatc aagcctgcat
    14821 acctgaggct tcatcgtaat ttttacgtcc gttatcgcca cttaccagta gccatttcac
    14881 ttttccggcg tggtacaacg ctgctgcggt atcaatacgt cgggtaaagt agcgattacc
    14941 cggcctggcc cccagcaata agcccacgtt acgcgccgga actgcgttga catcgctcca
    15001 ggtcagctgc ttgctggcgt tgaccataac gcgatcggca ataaaaatgg tcgctcctgc
    15061 tataagcaac aacaaacagc caatcttaag agtccggcga gaaaaacaaa tacggagaag
    15121 aaggcggggc tgcatggtcg ttatccttaa cgaaaggggg gaggtcgatt ttaggcgcta
    15181 aagaaagaaa agataacgcc tgacatcatt ttcagatttt gtcctgccca agcgacaact
    15241 gcgttagcca cagccgggtg tcgaactcaa gctggtggta ctgcggctcc atatggcagc
    15301 acaactggta aaacgctttg ttatgctctt tctccttcag gtgcgccagt tcgtgcacca
    15361 cgatcatacg taaaaacggt tccggcgcgt tgcgaaacac agtagcgacg cggatctctg
    15421 cttttgcttt cagcttgccg ccctgcacac gagaaacagc ggtatgtaac ccgagcgcgt
    15481 tttttagcac atggatctta ttgtcataca tcactttatt gatcggcggg gcattgcgca
    15541 gaaactgatt ttttagatcc ggagtatatt gccagagggc tttatcggtg gcgtagtcgt
    15601 gtgttcccgg atagcgtttt gccagcacat cgcccagacg ctgttcgtta atcagcgttc
    15661 gcacctggga aagtagctgc tcgggataac cctgaagata agtaagattg ctcataaccg
    15721 cccacgaaaa agagaaaaag ggtatactca cgcacccttt tcaggggcaa cgtcgaaatt
    15781 ttatcattca ggagggccga tgagccactt agacaacggt ttccgttcac tgacactaca
    15841 acgttttccg gcgacggatg acgttaaccc gctacaggcg tgggaagcgg cggatgaata
    15901 tttgctgcaa cagttggacg acacagaaat ccgcggcccg gtgttgatcc tgaatgatgc
    15961 ctttggtgcg ttaagctgcg cgctggcgga acataagccg tacagcatta gcgactcata
    16021 catcagtgaa ctggcgacgc gcgagaattt acgcctcaac gggattgatg agtcgagcgt
    16081 gaagtttctc gacagcaccg ccgactaccc gcaacagccg ggtgtggtgc tgatcaaagt
    16141 gccgaaaaca ctggcattgc tggaacaaca actgcgtgcg ctgcgcaaag tggtcacgtc
    16201 ggatacacgt attattgccg gtgccaaagc ccgtgacatt cacacttcca cgctggaact
    16261 gttcgaaaaa gtgctcggcc cgaccaccac cacgctggca tggaaaaaag cgcgcctgat
    16321 taattgcacg ttcaatgaac cgccgctggc cgatgcgccg cagaccgtta gctggaagct
    16381 ggaaggtact gactggacta tccataacca tgcgaatgtc ttctcccgca ccgggcttga
    16441 tatcggcgcg cgcttcttta tgcaacatct gccagagaat ctcgaagggg agattgtcga
    16501 tctcggttgc ggtaatggcg ttattggtct gacgctgctt gataaaaacc cgcaggcgaa
    16561 agtggtgttt gtcgatgaat cgccgatggc ggttgcttcc agccgtttga acgttgaaac
    16621 caacatgcca gaggcgttgg atcgcagcga gtttatgatc aacaacgcgc tctccggcgt
    16681 ggagcctttc cgctttaatg ctgtgctctg caacccgccg tttcaccaac aacatgcgct
    16741 gaccgataac gtcgcctggg agatgttcca ccatgcccgc cgctgcctga aaatcaacgg
    16801 cgagctgtat attgttgcca accgtcacct ggattacttc cataaactga agaagatttt
    16861 cggcaactgc accaccatcg ccacgaataa taaatttgtg gtgctgaaag cagtgaagct
    16921 ggggcgtcgt cggtaagatt gggagttacc tgccggatgc ggcgttaacg ccttatccgg
    16981 cctacgttca gcacgttact cccaggccgg ataagacgcg gcaagcgtcg catccggcaa
    17041 tgtctgccca gttctggaat cgcgcttcca taaaacaaaa aatccctgtc accatcctct
    17101 acattctctt gtttagcgtt gttcaacgtt tatgctacat acgtgaagtt atgacgcata
    17161 aagaatacga tttctttacc gctgttcatc gtactaaggg gaaaaaatga ttgcgattgc
    17221 cgacatcttg caagcaggag aaaagctaac tgctgtggca ccttttctgg cgggtattca
    17281 gaacgaggaa caatacaccc aggcgctgga actggtagat catctgctgc tcaacgatcc
    17341 tgaaaacccc ttgctggatc tggtgtgtgc aaaaataacc gcgtgggaag aatcagcgcc
    17401 cgaatttgcg gaatttaatg ccatggctca agccatgcct ggcggtatag ccgtgattcg
    17461 tacccttatg gatcaatatg gtttaaccct ttccgatctg ccggaaattg gcagtaaatc
    17521 tatggtgtca cgcgttttga gcgggaagag gaaattaacg ctggaacacg ctaaaaaatt
    17581 ggcaacgcga ttcggcattt ctcccgcctt gtttattgat taacgcgacg tgccggatgc
    17641 gttgcatccg gcgcttcaaa taacgattta aatctccagc gccagccgtg ttccctgggc
    17701 aattgcccgt cgtgcgtcca gctccatagc cacatcgcag ccgccaatta aatgcacagt
    17761 tttcccgcta tcaatcagcg gttgcgccag cgcgcggttt ggctcttgcc ctgcgcagat
    17821 caccacattg tccactgcta atacctgcgt ttcgccgttg atcaccacat gcagcccgtc
    17881 atcgtcaatc ttctgataac ttacgcctgg gatcattttc acaccccgcg agagcagggt
    17941 ggtgcgatgg atccagccgg tggttttgcc taacccctgt cccggtttgc tggctttgcg
    18001 ctggagcatc acaatttgcc gtgggctacg cgggatctgc attccctgcg ggcttaagcc
    18061 accagcctgt tgtaggctgc tgtcgatccc ccactcatta cagaacctgg cgatattctg
    18121 gctggtggat tcgcccggct gacttaaata catcgccgta tcaaaaccaa tcccgccaca
    18181 accgatgatg gcaaccttgt tgcctaccgg cgctttgtcg cgcagtacat cgagataact
    18241 caataccttc ggatgatcga tcccgtcgat aggcggaacg cgcggcacga tcccactggc
    18301 gaggatcgtt tcatcgaacg cctgtaactg atccgccgtc acggtgtgat tgagtttcag
    18361 cgtcacgccc gtcacttcga tcatccggtg gtaatagcgc agcgtttcgt aaaactcctc
    18421 tttgccgggg atctgtttgg cgatattaaa ctgcccgccg atctcgctgt gggcatcaaa
    18481 caatgttaca tgatgcccac gcgccgccgc gttaatggca aacgccagcc cagcaggtcc
    18541 cgcaccgacc accgccagat tttttttctg cacggcagga aggattggca ttttggtttc
    18601 gtggcaggcg cgaggattca ccaggcacga ggtgactttg ccaacgaaga tctgatcgag
    18661 gcaggcctga ttgcagccaa tgcaggtgtt gatctcatcg gctcgccccg attgcgcttt
    18721 tgacagcagt tccgcatcag caagaaacgg tcgtgccatc gataccatat cggcatcgcc
    18781 gcgcgagaga atgtcgtcgg caacctgcgg atcgttaatc cggttggtgg tcaccagcgg
    18841 cagcgaaacg tggcctttca gtttgcgcgt gacccaagta aatgcgccgc gcggcacggg
    18901 cgtggcaatg gtcggaatac gtgcttcatg ccagccaatg ccggtgttga taatggtcgc
    18961 gcccgccgct tcaatggcct gcgccagctc taccgtttcg gcaaaagtcc cgccgtcttc
    19021 gaccaggtcg agcatcgaca gtcggtagat aataatgaag tcgttgccga cgcgttcgcg
    19081 caccgcacgc actacttcta cggcaaatcg catccggttg cggtaatcgc cgccccactg
    19141 gtcactacgc tgattggtgc gcagcgtcag aaattcgttg atcaaatacc cttcggaccc
    19201 cattacctct acaccgtcgt atcctgcctc ccgtgccagt tgcgcgcagt gggcgaaatc
    19261 gtcgatcagt tgcaggatct cttcatgggt gagttcgtga ggaacgaaac ggttgatggg
    19321 ggcctgcaat gcggaggaag cgaccagatg cggttggtag ctgtagcgcc cggtatgcaa
    19381 aatttgcagg gctattttgc cgccttcctg atgtaccgct tcggtaatgg tgcgatggtg
    19441 tgggatctgg ctggcgtcgt tgagcattgc gccaccttcc atgccaacgc ctgttaaatc
    19501 tggtgcaatg ccgccgctga caatcagcgc cacgccgtga cgggcgcgtt cggcataaaa
    19561 cgctgccagc cgctcggcac cgtccgggta ttcctccagc ccggtgtgca ttgagcccat
    19621 caacacgcgg ttttttaacg tggtaaaacc taaatccagc ggggcgaaca gcaacgggta
    19681 gctcataaaa ttgtccagta tgtaaaataa ttgttatgtg gtcggatgag ttctaattta
    19741 gccgtcgccg gagaaaaggg aaaaggggag tgcggggttt gtgatgggat tcaaaaagaa
    19801 tacgcccggt ggtaacgccg gatgcgacgc ttgccgcgtt ttatccggcc tgggggattg
    19861 cacatgtagg gcggataagg cgttcacgcc gcatccgcca gtggcacggt gcagatgccg
    19921 gatgcgacgc ttgtcgcgtt ttatccggcc aggaggattg cacatgtagg gcggataagg
    19981 cgtttacgcc gcatccgcca gcagttgcaa attattgctt acggaaaaaa tcgttataca
    20041 acatatacaa atgcgcggca ctccaggaga aatttggtgc gccttgctgt gcgccagtca
    20101 gcgggttgta attctcctga attgggccat cggcggttaa tcctttggcg tgacggaaga
    20161 acgtatccgc cagtttcagg gcatcatcac gataaccgta acgctccatc cctttcagac
    20221 caaaccagaa ctgatccacc catacgcgcc cgcgccagta gatatcagcg ccaaaagccg
    20281 gattggttaa cgccgccgtt cccagcggga caaaggtgtt gaactcttta ggatcgagca
    20341 tcaccttcac caccgcgtcg gcgttggcct gcgttgccgc accgttaaac agcggcgacc
    20401 agccttccgg ccctttaccg cgctcgacaa tcggtttgcc cgcgcagccg ttcgccagcg
    20461 gtttatcttc aatacgcacg tcatagtaga actgggtagc cgggtcgaac atacaggtgt
    20521 ttatgtagtc tgcgagctgc tgtgccaact ggcgatagcg tttggcctct tccggcttac
    20581 cgaggatggt tgccatctcc gccagataat ggttatcgct gtacatatag ctggcctgat
    20641 ccaccgactc ctgcaatagc gagtagccca gcaacgttcc gtcctgactg cggttttcgg
    20701 cgaatttcac cgtccagtcg ctacgtttgc cgccattagc gacatattta tccaactgtt
    20761 ctttgtcgat aaaaccaaag acggcggcat catcacgccc cgattcccac gacgcagcga
    20821 cctgtgccgg aatttccaga ctgtcgtact ggcctttctc caccacgcgg gcgtagttgt
    20881 tcagcccaga ctgcgtctct tctttgttgc ctttcttcac cgtaaacagc atctcgccgc
    20941 tctcagtgtt gtgggctttg tcgcgggtcg cgccatatta cggcacgccg ttgccgttgt
    21001 gatcgcggtt acgtaaccac cagtcgtgat aggccaccag tttcgggtac atctctgcca
    21061 accaggcttt atcctgggtg acgttgtaca cttccatcac cgaccaggcg gcaaggctgg
    21121 gtttggtatt acgttcgttc cagttgccgc catcgccacc acgctcgggg ctaaggttcc
    21181 acgctatcag gtcggggaca aagcccacat cctgcggacg cacgctatcg ccaggctgga
    21241 tctgccagga gaagaccgcg cggatattct ctttggcgat gtccggattg aaatgcgcca
    21301 tcgcaaacgc ctgcttccag gtatcccacg gccaggtctg attgccggag aaccagcgcc
    21361 cggtcaccga cggtgtaacg gtgttaaatt tcaccgcacc gcccggtgag cgccagttac
    21421 cgttgagcgt ttcgatcgct ttcaccgcga cgcgcgtctg ttccggcgtc gcatccggat
    21481 tggttaaccc tttcttcaga tactcttccc agcgttgctg cgaggcggtg agataaaacg
    21541 ccggacgcgc cagaatatcg cggatctgca tttgctcttt gctaacttcc tgagaggtca
    21601 gcagatggga ataggtggta tagagcgtcg tcgaaccgtt gatatgtgcc ttactggtaa
    21661 agcgattgcc gttgatttca gtctgcaccg gcagggattt atgcacctga tattccgatt
    21721 cgccggaggt cagcagatcc caggtggcgc gcactttgcc aaaggtgact ttcaggccat
    21781 cacgggtggc gctgattttg cgctggtagt cagggtattc gccagcaatg gttttatcgg
    21841 aaagcggttt cccttctttc gcttccagtt tttccagcag ttcgccgtcc cacaccagat
    21901 ccagcggttt attgctggtg attttggttt ccagtagtga cgtgcgcggc gtggcgaagc
    21961 gcagagtcat ttcgacttgc acatcttttg ctgtcagttt ttgcaccagc gcaccgggaa
    22021 tactgtatgc ctccagcgta aagtcgactt tcttgccgtc ctgccagacg gtcaggcggt
    22081 cgaaattgct ggccataaag ttgatgtact cttccgtcag cagcgcaacg cccggaaagc
    22141 cgcccatggt gttagggccg tctggcaaca gatgaccatg ccaggctccg agatcgaaaa
    22201 acggattaaa acgctggtga tcgtcgtaat cgtaatcctt catgtactgc ggcgcgccag
    22261 tacggttaat cacgttttta taattgtcgg cgttagcggc atgggcggaa aaacttatca
    22321 gcagagcgca ggttactggc gttaaaatag tttttatttt catgaaatta cagtccttat
    22381 taccagtaga aatattgcat caggaagatt tggtcgctgg gatcgtgaga ggcatttttc
    22441 atgtagaagc ggctatcaaa agccgtggcg aaacgtccgc cgccgtattc gtaccagata
    22501 ccaaactgca tgaaagaggt ggtttcatcg tcagcgtggt cagggcgatg tttggcgtag
    22561 gtgtaagcgg tggagagata aacgcccttt aacgcttcat acatggcgct caccatcatc
    22621 ccactttcac ttcccggtac gtccagcccg tcggcgcgtc cggtatggtg ccaggcgcga
    22681 gcggcaaagt taggagttag caggcccgtg agatagagct gtcctgtgcc gtcggtgatt
    22741 tccagaccgt tcatccaggt gatgccttcg gcaaggtcat actggatgta accgttcatc
    22801 attgccggat aggtgtattt gtcgtcatag cgatcgtact tgccaaaatg tagccaggct
    22861 ttgctttcgt catgatgtcc ggcaggtgtg gcggtgacgc tataacgaaa gttgccgctt
    22921 aagttctgca ccttcagcgc ggtcataatg tcgcgggtat tgggaatgac gtagcccaga
    22981 tcgggggtga agtcgcccca ccattgcagg tcatccagcg aggaatcctg acgcagactg
    23041 agcatgattt cggtgccatc attggtacga taaccgccat agaaacggtt aattccgccc
    23101 tcgaaaccac cccagctatg gtctggcacc caggcattgc cgtcgtggtc ggcctgcacg
    23161 gtccagcctt cgccgtaaat caggccgaac caggagccgt acttcatctc caggccacct
    23221 tcaatatagg taccgtcgct gtatttatgt ttattctcac cctctaaatc catatatcca
    23281 ccaaccccta attcgccgta aaaacgaaat gaaggagaag atgtcgaggt aattgcaggt
    23341 gtttttgtgt cgtcatgttc tgccggacgt gcttcttctc ctgccgcaga aaaggcgtag
    23401 caaaacggca gagcaagtaa tgctctgcat ggtgcagtg
//
LOCUS       DAZKHF020000050        23392 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2201S31241
            SAMN43267800-rid25106413.denovo.050, whole genome shotgun sequence.
ACCESSION   DAZKHF020000050 DAZKHF020000000
VERSION     DAZKHF020000050.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267800
            Sequence Read Archive: SRR30307263
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 23392)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23392)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 23392)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350581.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350581.2
            Genome Coverage       :: 151x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:21:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,652
            CDSs (total)                      :: 5,557
            Genes (coding)                    :: 5,320
            CDSs (with protein)               :: 5,320
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 237
            CDSs (without protein)            :: 237
            Pseudo Genes (ambiguous residues) :: 0 of 237
            Pseudo Genes (frameshifted)       :: 102 of 237
            Pseudo Genes (incomplete)         :: 130 of 237
            Pseudo Genes (internal stop)      :: 52 of 237
            Pseudo Genes (multiple problems)  :: 41 of 237
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23392
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267800-rid25106413.denovo.050"
                     /serotype="O 26:H11"
                     /isolate="2201S31241"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-01"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            130..975
                     /locus_tag="ACHOSC_RS07980"
                     /old_locus_tag="pgaptmp_001596"
     CDS             130..975
                     /locus_tag="ACHOSC_RS07980"
                     /old_locus_tag="pgaptmp_001596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000022370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix domain-containing
                     protein"
                     /protein_id="HGU4547558.1"
                     /translation="MSIIINNWRMDPSLNALIHCETGETRRLGEYHFILLEKLAKNAD
                     VVLSRSYLCAEVWKNRIVGGNSLPTAIHALRVAIDDDDGKQQNIIKTIPKKGYLCNKE
                     YVSLPDSSPAKKLIITDQIQETVPEEISSTTPPVPVRKKHKVIMGLALAAAVIFIGST
                     VGYSHLKSTPDAPQLVKESINSPRIKIFHLSSGKENNSVPLLSQTLAPGKDKLDNLLS
                     AHNMTMTTYYKYVRNRLESDIVLRNQCNGSWQLTFNVEGWQNSDINSAMYQNLEKLLN
                     TLQKC"
     gene            complement(1030..2007)
                     /locus_tag="ACHOSC_RS07985"
                     /old_locus_tag="pgaptmp_001597"
     CDS             complement(1030..2007)
                     /locus_tag="ACHOSC_RS07985"
                     /old_locus_tag="pgaptmp_001597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001195949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-associated protein"
                     /protein_id="HGU4547559.1"
                     /translation="MQVGLTSSSLATGGAHSAAVSSSTVAPTQAVRQKLPATASEYPA
                     SPLITTRPQRYSVQLNDQLTTLQQADHYLGQLEQQLLDYRHSQRKGGQAQNTALMQML
                     DKRTALSGGAVDRQLQPVLQGEARVTFHSPDLANLVHNPTPGTRMFSVSDGRQTQLSA
                     VMLSEDDSAAQYQTRLTNALRRVGVQLHQQADGISFSTTEKQWPHIERTLSVRTDGDK
                     SAFMPLKTFAEPSEADRLAQSLQQGGAGISQTLESINQQRAQMAVQQEKARQLIDGMS
                     RFPQTENAVQASENLGGVLDSANHNYQVLLQAVNGQARISSQTVRNLLG"
     gene            complement(2024..2953)
                     /gene="flgL"
                     /locus_tag="ACHOSC_RS07990"
                     /old_locus_tag="pgaptmp_001598"
     CDS             complement(2024..2953)
                     /gene="flgL"
                     /locus_tag="ACHOSC_RS07990"
                     /old_locus_tag="pgaptmp_001598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020219239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009424 - bacterial-type flagellum hook
                     [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-associated protein FlgL"
                     /protein_id="HGU4547560.1"
                     /translation="MRVTTQQTYVSMTQSFNNLSGDLAHVVEQMATGKQILQPSDDPI
                     AATRITQLNRQQSAIEQYQSNIDSASAGLSQQESILDGVNNSLLAVRDDLLEAANGTN
                     TADSLASLGQDIESLTESMVAALNYQDEDGHYVFGGTINDQPPIVAVDDDGDGVTDSY
                     SYQGNSDHRQTTVSNGVEVDTNVAASDFFGSNLDVLNTLNSLSQELQNPDVDPADPQV
                     QSDIQNAVDVVDTASDDLNASIASLGETQNTMSMLSDAQTDISTSNDELIGSLQDLDY
                     GPASITFTGLEVAMEVTLKTYSKVSELNLFSVL"
     gene            complement(2968..4344)
                     /gene="flgK"
                     /locus_tag="ACHOSC_RS07995"
                     /old_locus_tag="pgaptmp_001599"
     CDS             complement(2968..4344)
                     /gene="flgK"
                     /locus_tag="ACHOSC_RS07995"
                     /old_locus_tag="pgaptmp_001599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000367241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009424 - bacterial-type flagellum hook
                     [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0044780 - bacterial-type flagellum assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-associated protein FlgK"
                     /protein_id="HGU4547561.1"
                     /translation="MDMINIGYSGASTAQVELNVTAQNTANAMTTGYTRQVAEISTIG
                     ASGGSPNSAGNGVQVDSIRRVSNQYQVNQVWYAASDYGYYSTQQGDLSQLEAVLSDDN
                     SSLSGGFDNFFAALNEATTSPDDSALREQVISEAGALSLRIDNTLDYIDSQSTEIISQ
                     QQAMVSQINTLTSGIASYNQQIAQAEANGDNASALYDARDQMVEELSGMMDVQVNIDD
                     QGNYNVTLKNGQPLVSGQQSSTIALETNADGTSTMSLTFAGTTSTMTTDTGGSLGALF
                     DYQNDVLTPLTDTINSMASQFADAVNNQLAQGYDLNGNPGEPLFIYDASNADGPLTVN
                     PNITADELAFSSSPDESGNSDNLQALINISTEPLEIANLGSVTVGQACSSIISNIGIY
                     SQQNQTEVDAASNVYSAAQNQQSSVSGVSMDEEAVNLITYQQIYEANLKVISAGAEIF
                     DSVLEMCS"
     gene            complement(4526..4825)
                     /locus_tag="ACHOSC_RS08000"
                     /old_locus_tag="pgaptmp_001600"
     CDS             complement(4526..4825)
                     /locus_tag="ACHOSC_RS08000"
                     /old_locus_tag="pgaptmp_001600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000867283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod-binding protein"
                     /protein_id="HGU4547562.1"
                     /translation="MKVNASGGIDGSDALMGPKVQANDIKQAAEQFEAIFLRNMLKEM
                     RKTNELFDSKDNPFNSDSVRMMQGFYDDELCNTLAQQHGIGIAAMIVKQLSPKHK"
     gene            complement(4825..5925)
                     /locus_tag="ACHOSC_RS08005"
                     /old_locus_tag="pgaptmp_001601"
     CDS             complement(4825..5925)
                     /locus_tag="ACHOSC_RS08005"
                     /old_locus_tag="pgaptmp_001601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001181884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009428 - bacterial-type flagellum basal
                     body, distal rod, P ring [Evidence IEA];
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body P-ring protein FlgI"
                     /protein_id="HGU4547563.1"
                     /translation="MQNWIKAAVIAVSLALPGVALAQSLESLVNVQGVRENQLVGYSL
                     VVGLDGTGDKNQVKFTNQTITNMLRQFGVQLPNKIDPKVKNVAAVAVSATLPPMYSRG
                     QTIDVTVSSIGDAKSIRGGTLLLTQLHGADGEVYALAQGSVVVGGMNATGASGSSVTV
                     NTPTAGLIPNGATVEREIPSDFQMGDTITLNLKRPSFKDANNIAAAINASFGGIATAQ
                     SSTNVTVRAPTSPGARVAFMSQLDDVQVQAEKIRARVVFNSRTGTVVMGDGVALHAAA
                     VSHGSLTVSINETSNVSQPNAFAGGRTAVTPQSNIAVNHARPGVVSLPESSSLKTLVN
                     ALNSLGATPDDIMSILQALHEAGALDADLEVI"
     gene            complement(5940..6605)
                     /gene="flgH"
                     /locus_tag="ACHOSC_RS08010"
                     /old_locus_tag="pgaptmp_001602"
     CDS             complement(5940..6605)
                     /gene="flgH"
                     /locus_tag="ACHOSC_RS08010"
                     /old_locus_tag="pgaptmp_001602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002211429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009427 - bacterial-type flagellum basal
                     body, distal rod, L ring [Evidence IEA];
                     GO_function: GO:0003774 - cytoskeletal motor activity
                     [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body L-ring protein FlgH"
                     /protein_id="HGU4547564.1"
                     /translation="MKNYLWLVALLPLLSGCESQAILVKKDDAYFAPPKTEAPPPADG
                     RAGGVFETGYNWSLTADRRAYRVGDILTVILEESTQSSKQAKTNFGKSNTVDIGAPTI
                     FSHTKDKLSGSIDANRDFDGSATSQQQNSLRGEITVSVHAVQPNGILEIRGEKWLTLN
                     QGDEYIRLSGLVRADDIQNDNSVSSQRIADACISYAGRGALSDANAAGWLTRLFNHPL
                     FPI"
     gene            complement(6688..7473)
                     /gene="flgG"
                     /locus_tag="ACHOSC_RS08015"
                     /old_locus_tag="pgaptmp_001603"
     CDS             complement(6688..7473)
                     /gene="flgG"
                     /locus_tag="ACHOSC_RS08015"
                     /old_locus_tag="pgaptmp_001603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003838810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009426 - bacterial-type flagellum basal
                     body, distal rod [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal-body rod protein FlgG"
                     /protein_id="HGU4547565.1"
                     /translation="MNAALWISKTGLSAQDAEMSAIANNIANVNTTGFKRDRVMFQDL
                     FYQTQEAPGAMLDQNNIMPTGLQFGSGVRIVGTQKTFTEGNVETTDNAMNVAIMGQGF
                     LQVQKANGDIAYTRDGNLQVNADGVLTNSQGLPLQPEIDVPAGATNVAFGEDGTVTAI
                     LPGDSDVTELGQLTLVNFANPAGLSAEGDNLYLETAASGQPTEGVPGEDGLGTLQDNA
                     LEGSNVDIVNEMVAMITVQRAYEMNAKMVSAADDMLQYISQTL"
     gene            complement(7552..8289)
                     /locus_tag="ACHOSC_RS08020"
                     /old_locus_tag="pgaptmp_001604"
     CDS             complement(7552..8289)
                     /locus_tag="ACHOSC_RS08020"
                     /old_locus_tag="pgaptmp_001604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000375458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body rod protein FlgF"
                     /protein_id="HGU4547566.1"
                     /translation="MDRLIYTALSGASQTLYEQQISANNLANVNTNGFRADMAMATND
                     KVKGGGFDTRYMAQEGASGVNDSTGVAEKTERPLDVAIQGAGYIAVQDKNGNEVYTRN
                     GNIQQDDQGQLTIDGNLVLGDNGPIILPPNAIASFGSDGTLSVTPDDGDVTATMDIDR
                     LKLVDIPVANLEKNAEGMLVTADGVPAQRDENIKVSGGFLEGSNVSAVSEMMSSIAMN
                     RQFEAQIKMMKTAEDISDAGNRLLRGS"
     gene            complement(8289..9491)
                     /gene="flgE"
                     /locus_tag="ACHOSC_RS08025"
                     /old_locus_tag="pgaptmp_001605"
     CDS             complement(8289..9491)
                     /gene="flgE"
                     /locus_tag="ACHOSC_RS08025"
                     /old_locus_tag="pgaptmp_001605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012904671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook protein FlgE"
                     /protein_id="HGU4547567.1"
                     /translation="MSYEIAATGLNAVNEQLDGISNNIANAGTVGYKSMTTQFSAMYA
                     GSQAMGVSVAGTAQSISRGGSLVSTGNALDLAINDDGFFVMCDSAGNISYTRAGSFET
                     DKNGYIVNASGAYLQGYPVDDTGTLQTGTVTDIQIKTGNIPAQASSSLTFTANFNASD
                     EAIDRTTVPFDATNSSSYTDSYTTTVYDSLGNEHSVCQYFTKTSDNTWEVQYTFDGQQ
                     QTGVPATTLTFDPNTGKLTSPTTPQTIEFQTDAAAPVDLTVDYSTCTQYGSEFSVTTN
                     AANGYASATQNGVQVDDDGKVYATYSNGERMLQGQVVLATFPNENGLEAVSGTAWVQT
                     GESGTPLIGVPGSGTCGTLSSGVLESSNVDITSELVNLMTAQRNYQANTKVIATSTQL
                     DDALFQAM"
     gene            complement(9515..10228)
                     /locus_tag="ACHOSC_RS08030"
                     /old_locus_tag="pgaptmp_001606"
     CDS             complement(9515..10228)
                     /locus_tag="ACHOSC_RS08030"
                     /old_locus_tag="pgaptmp_001606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001508388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook capping FlgD N-terminal
                     domain-containing protein"
                     /protein_id="HGU4547568.1"
                     /translation="MNTLALNAQSPQPTATAESNNVAASDTQSSSDSSPVDTFLTLFV
                     AEIQNQDPTDPTDATEYIDQLSSMAQVAMMEEMSVQANTNAVLMSNIQVMALGNLVGD
                     DIMVQTTALQVSDQTINGRATLDDACTTADLHFTDAAGDDYTVSLIPEGSSSVGPGQV
                     DFSVNPADYGIPPGDYDVSVVTNTGEEEVPIEVSGEVEDVRIPLDGSSPVLNVAGVGE
                     VPFTMISQFGVPDADSDVA"
     gene            complement(10228..10659)
                     /gene="flgC"
                     /locus_tag="ACHOSC_RS08035"
                     /old_locus_tag="pgaptmp_001607"
     CDS             complement(10228..10659)
                     /gene="flgC"
                     /locus_tag="ACHOSC_RS08035"
                     /old_locus_tag="pgaptmp_001607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000010944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030694 - bacterial-type flagellum basal
                     body, rod [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body rod protein FlgC"
                     /protein_id="HGU4547569.1"
                     /translation="MSFTDIYQISGSAMTAQTLRLNTVASNLANANAPASSEAQAYKA
                     RSPVFAAVYHHSLLAGTHRHAIDGASVQVQDVLQTGGAVKRYEPHSPLADANGDVWYP
                     DVNVVEQMADMMSASRDFETNVDVLNNVKSMQQSLLKLGEA"
     gene            complement(10662..10997)
                     /locus_tag="ACHOSC_RS08040"
                     /old_locus_tag="pgaptmp_001608"
     CDS             complement(10662..10997)
                     /locus_tag="ACHOSC_RS08040"
                     /old_locus_tag="pgaptmp_001608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002211435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body protein"
                     /protein_id="HGU4547570.1"
                     /translation="MGISFQQALGVHPQAVKLRLERTELLTANLANVDTPNFKAKDID
                     FAREMQRANNAAVDVQYRVPMQPSEDGNTVELNSEQARFSQNSMDYQSSLTFLNLQIS
                     GIREAIEGK"
     gene            11079..11816
                     /gene="flgA"
                     /locus_tag="ACHOSC_RS08045"
                     /old_locus_tag="pgaptmp_001609"
     CDS             11079..11816
                     /gene="flgA"
                     /locus_tag="ACHOSC_RS08045"
                     /old_locus_tag="pgaptmp_001609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016233868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009428 - bacterial-type flagellum basal
                     body, distal rod, P ring [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body P-ring formation chaperone
                     FlgA"
                     /protein_id="HGU4547571.1"
                     /translation="MSFQFCRRKFYFLLALALPGYAAVHPVQHSAREQVNAQVLNAAS
                     QKIESLAQQRQWHDYRYTFKIYIPSQIATAAPCTKTPGVTLTSPAEIALNRMNFTVSC
                     PQSWQMNVAVRPDVLVPVVMTKSLVARDMPLTANDVELKPYNVSAQRRDVLMELDDAI
                     GLSSKHALQPGRPITKEELVSPVLVGRDQPVMIVYQSAGITASMPGVALKNGRKGEMV
                     KVRNASSQRVISAMVAESGVVTTVSAE"
     gene            11897..12175
                     /gene="flgM"
                     /locus_tag="ACHOSC_RS08050"
                     /old_locus_tag="pgaptmp_001610"
     CDS             11897..12175
                     /gene="flgM"
                     /locus_tag="ACHOSC_RS08050"
                     /old_locus_tag="pgaptmp_001610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000705216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis anti-sigma factor FlgM"
                     /protein_id="HGU4547572.1"
                     /translation="MKITPTMPGNRPTATTAGSSQPRKTTVTTTTTLSADDITQAGLQ
                     SAQQTLNDQQESDIDSNKVAQVQAMLASGSPQVDTEQLASDMLSFFQN"
     gene            12188..12616
                     /gene="flgN"
                     /locus_tag="ACHOSC_RS08055"
                     /old_locus_tag="pgaptmp_001611"
     CDS             12188..12616
                     /gene="flgN"
                     /locus_tag="ACHOSC_RS08055"
                     /old_locus_tag="pgaptmp_001611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001329715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0044780 - bacterial-type flagellum assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar export chaperone FlgN"
                     /protein_id="HGU4547573.1"
                     /translation="MSIRLQQVKALLQGIRDDDALYDSLRELLQRQRICMIRRASEEL
                     LAVNDEITHHYEQLHGHSHQRHSLLKMLGVSVNRDGLAQVFTWLPAVQKAAAQQLWQR
                     LEQKAERCKAYNDKNGELLIRQYEFIQSFLGSEADFLYQE"
     gene            12743..13672
                     /locus_tag="ACHOSC_RS08060"
                     /old_locus_tag="pgaptmp_001612"
     CDS             12743..13672
                     /locus_tag="ACHOSC_RS08060"
                     /old_locus_tag="pgaptmp_001612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000008086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine-N-methylase"
                     /protein_id="HGU4547574.1"
                     /translation="MSFIECYEPEYVRKFLARPPGSPLYVRKVWKNEIRLSLSLTDIA
                     SCEAVLNDVRAFDLQLTYRPVEDNSAELSARDAIVNQVILSTLTLRDLTPELSLYAVG
                     ILLSCASKMPGRDGDILARLTTLPQALADHAKKGTLQAQYAQLPPVPQLARQLMTLLG
                     GCAFDWSILPESPRKASLPLQVTLLTLHDANSEALLQQQLQTQWQTTWQQHFATAPWM
                     MRNWLIYRVYHDMIGQTDGADYFPLVCDFYLLRTLISLWTLDGSPLRQEDIFALFAMF
                     ERWRASENALLVRQQIQSLCAADPLLSAFSLLT"
     gene            13851..16295
                     /locus_tag="ACHOSC_RS08065"
                     /old_locus_tag="pgaptmp_001613"
     CDS             13851..16295
                     /locus_tag="ACHOSC_RS08065"
                     /old_locus_tag="pgaptmp_001613"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001412594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HGU4547575.1"
                     /translation="MPTPTIIKTLATHREKPFVSVVTPTWNRGAFLPYLLYMYRYQDY
                     PADRRELIILDDSPQSHQHIIDRLTNGTPEAFNIRYIHHPEKLPLGKKRNMLNELARG
                     EYILCMDDDDYYPADKISYTIAMMQKHRALISGSDQIPIWYSHINRIFQSRSFGPHNI
                     LNGTFCYHRNYLKKHRYDDDCNLGEEKSFTDNFSVNPLQLPGERTILCISHSHNTFDK
                     DFIIGASTPVNTALTDIVRDPLLRNAYLSLHNATHHQAINHQAIDQVVVVNLDKRPDR
                     LQQIREELALLHIPPEKITRLAASEDENGQRGRRQSHLQALRLAQQHGWQNYLLLEDD
                     AVILKQEKHIQVLNTLLASLAKIPWQVMILGGEISQGTMLKSLPGLVHARDCRKVCAY
                     LVNSRYYPQLAQQMSNDEHSLEDCWQPLLRADKWLACYPSLCYQRPGFSDIQKKTTDN
                     IARYFNKLPVTTKPSALPIADTIGFFMETSFHYALYRPIITALQAQGLSCTLVINDRV
                     FKPFLDEMLETLKNIDDPQLKGMRLSEMQTHGQRVKCLVSPYHTPALNGLAAVNIRAM
                     YGLAKETWNHADWNRFYQSILCYSHYSQQALAHFGSAKVVGNPRFDAWHNGTFDRALP
                     ENIQSDYRKPTVLYAPTFGALSSLPHWAEKLGRLSGDVNLICKLHHGTCSRPEEAASL
                     ALVRRHLKQRTDSARHTLALLAKADYVLTDNSGFIFDAIHVDKRVILLDFPGMNDLLD
                     GEKSYSTAESADQRIREILPVAHDVAELRYLLSEAFDWGSVQARLTEIRHHYCDAFMD
                     GKAGERAAIVIVEALG"
     gene            complement(16413..16808)
                     /locus_tag="ACHOSC_RS08070"
                     /old_locus_tag="pgaptmp_001614"
     CDS             complement(16413..16808)
                     /locus_tag="ACHOSC_RS08070"
                     /old_locus_tag="pgaptmp_001614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001637450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylyltransferase/cytidyltransferase family
                     protein"
                     /protein_id="HGU4547576.1"
                     /translation="MKTVITFGTFDVFHVGHLRLLQRARALGERLLVGVSSDALNIAK
                     KGRAPVYHQDDRMAIIAGLACVDGVFLEESLEQKAEYLRGYSADILVMGDDWAGKFDS
                     FAYICEVVYFPRTPSVSTTGIIEVIRGLL"
     gene            complement(16811..17245)
                     /gene="fliJ"
                     /locus_tag="ACHOSC_RS08075"
                     /old_locus_tag="pgaptmp_001615"
     CDS             complement(16811..17245)
                     /gene="fliJ"
                     /locus_tag="ACHOSC_RS08075"
                     /old_locus_tag="pgaptmp_001615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001248915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009288 - bacterial-type flagellum
                     [Evidence IEA];
                     GO_function: GO:0003774 - cytoskeletal motor activity
                     [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar export protein FliJ"
                     /protein_id="HGU4547577.1"
                     /translation="MRQIIDTLTQLQRLRDKSVKDKTVELAKQKQICAGYDNNIKALG
                     YLVEKTSAGAAVSVESLKNVSGYKGTLRKVIAWQEQEKTLANIKATRMQKNLTAAACE
                     EKVVALTLDDKRREQQESATAKAQKAVDDIAVQCWLRHKLAE"
     gene            complement(17248..18585)
                     /gene="fliI"
                     /locus_tag="ACHOSC_RS08080"
                     /old_locus_tag="pgaptmp_001616"
     CDS             complement(17248..18585)
                     /gene="fliI"
                     /locus_tag="ACHOSC_RS08080"
                     /old_locus_tag="pgaptmp_001616"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001279390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0030254 - protein secretion by the type III
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar protein export ATPase FliI"
                     /protein_id="HGU4547578.1"
                     /translation="MSDFSAFDNALRSLESIPLARVAGRLVRLNGILLESVGCPLMTG
                     QLCRIESANHTLIDAQAVGFNRDITYLMPFKQPVGLMAGARVFPEEKAHDILIGESWL
                     GRVVNGLGEPLDGKGRLNGNDLLPPLPPSVNPLTRRSVDEPLDVGVKAINGLLTIGKG
                     QRVGLMAGSGVGKSVLLGMITRQTKADIVVVGLIGERGREVKEFIDHSLGADGLAKSI
                     VVVAPADESPLMRLKATELCHSIAAWFRDRGHHVLLLVDSLTRYAMAQREIALSLGEP
                     PATKGYPPSAFGMIPKLVESAGNSESAGSMTAIYTVLAEGDDQQDPIVDCARAVLDGH
                     IVLTRKLAEAGHYPAIDIGQSISRCMNQVTSLEHQQSARALKQNYAAYMEIKPLIPLG
                     GYVAGADASVDKAVKMFPAIERFLRQEMREPASLELVQSRLQILFPGVKKAEQ"
     gene            complement(18578..19288)
                     /gene="fliH"
                     /locus_tag="ACHOSC_RS08085"
                     /old_locus_tag="pgaptmp_001617"
     CDS             complement(18578..19288)
                     /gene="fliH"
                     /locus_tag="ACHOSC_RS08085"
                     /old_locus_tag="pgaptmp_001617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001521882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar assembly protein FliH"
                     /protein_id="HGU4547579.1"
                     /translation="MTIETLRGRYRLHRFPPRQRAVTGEPLTTSISAADYQRQLMDGF
                     QEGLQKGFAQGMTEGQEQGFSEGHQQGFAEGRRQGYTEGSLAGQQEGRKQFVEAAQPL
                     EAITGKVNDFLAHIERKQREDLLQLVEKVTRQVIRCELALQPTQLLALVEEALAAFPA
                     MPETLQVMLSTEEFNRLRDAVPEKVSEWGLTPSPDLPPGECRVITDKSELDIGCEHRL
                     EQCMTALKETLTPESQGE"
     gene            complement(19291..20301)
                     /locus_tag="ACHOSC_RS08090"
                     /old_locus_tag="pgaptmp_001618"
     CDS             complement(19291..20301)
                     /locus_tag="ACHOSC_RS08090"
                     /old_locus_tag="pgaptmp_001618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001293121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor switch protein FliG"
                     /protein_id="HGU4547580.1"
                     /translation="MSELTTNKSNSYLEQAAILLLCLGEEAAATVMQKLSREEVVRLS
                     ENMARLSGVKTSMARKVINNFFDEFREQSGINGASRSMLQGILNKALGTEIASSVING
                     IYGDEIRSRMARLQWVEPRQLAMLISEEHLQLQAVFLAFLTPEISAAVLSYLNESVQN
                     EILYRVAKLNDVNRDVVDELDRLIERGLSVLSEHGSKVKGIKQAADIVNRFQGNQQVI
                     LDQMRERDEEVLEQLQDEMYDFFILSRQNEEVRRRLLDEVPMEDWAVALKGTEALLRR
                     SIYAVMPKRQAQQLEAITARLGPVPVSRIEQIRREIMGIARELEEAGEIQLQLFAEQT
                     AE"
     gene            complement(20279..21925)
                     /gene="fliF"
                     /locus_tag="ACHOSC_RS08095"
                     /old_locus_tag="pgaptmp_001619"
     CDS             complement(20279..21925)
                     /gene="fliF"
                     /locus_tag="ACHOSC_RS08095"
                     /old_locus_tag="pgaptmp_001619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001643010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009431 - bacterial-type flagellum basal
                     body, MS ring [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal-body MS-ring/collar protein
                     FliF"
                     /protein_id="HGU4547581.1"
                     /translation="MNAQIKKLTLAFPAFRLRLADNKRWALMAGVGLAVAATAIIVSV
                     LWTGNRGYVSLYGRQENLPVSQIVTVLDGEKLTYRIDPQSGQILVPEEALSKTRMTLA
                     AKGVQAILPSGYELMDKDEVLGSSQFVQNVRYKRSLEGELAQSIMSLDAVESARVHLA
                     LNEESSFVVSDEPQNSASVVVRLHYGAKLNMDQVNAIVHLVSGSIPGLHASKVSVVDQ
                     AGNLLTDGIGAGEAVSAATRKRDQILKDIQDKTHASVANVLDSLVGSGNYRVSVMPDL
                     DLSTIDETQEHYGDAPKINREESVLDSDTNQVAMGVPGSLSNRPPVAANQMTNGTEEN
                     RSPEALSKHSESKRDYSYDRSVQHIQHPGFAVKRLNVAVVLNQNAPALKNWKPEQTAQ
                     LTALLNNAAGIDAQRGDNLTLSLLNFVPQAVPVEPIIPLWKDDSVLAWVRLIGCGLLA
                     LLLLFFVVRPVMKRLTAVRAPIITPESEAVSEPWIAMPEEERKNVDLPSLPGDDSLPS
                     QSSGLEVKLEFLQKLAMSDTDRVAEVLRQWITSNERIDNK"
     gene            complement(21930..22271)
                     /locus_tag="ACHOSC_RS08100"
                     /old_locus_tag="pgaptmp_001620"
     CDS             complement(21930..22271)
                     /locus_tag="ACHOSC_RS08100"
                     /old_locus_tag="pgaptmp_001620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001538312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-basal body complex protein FliE"
                     /protein_id="HGU4547582.1"
                     /translation="MSITTINSTMQAQIMQDVQRMQANAQAPVLPAMTFSSTDPDVSF
                     HRIMQGALGHVDQFQQVAEQQQTAIDMGKSDDLAGAMIASQQASLSFSALVQVRNKIA
                     TGFNDLMSMSI"
     gene            complement(22286..23272)
                     /locus_tag="ACHOSC_RS08105"
                     /old_locus_tag="pgaptmp_001621"
     CDS             complement(22286..23272)
                     /locus_tag="ACHOSC_RS08105"
                     /old_locus_tag="pgaptmp_001621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001292201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 interaction domain-containing protein"
                     /protein_id="HGU4547583.1"
                     /translation="MSELIATAASSINAFTLAKRVAAFNVPVLIQGETGAGKECVAKY
                     IHTVAFGENENAPYIGVNCAAIPENMLEATLFGYDKGAFTGAIASVPGKMELANNGTL
                     LLDEIGDMPLALQAKILRVLQEQQVERLGSNRQIKLNFRLIACTNKNLEQEVAAGRFR
                     EDLYYRLAVIPITMPPLRERLNDIIPLAESFIKKYSTVLVKNITLSESTRRAMLNYRW
                     PGNVRQLENAIQRGMILNRDGVIYPDALGLPENDIADHSELQWPAQPAVHIAETSDLG
                     QHGRSAQYQYIADLMRKYQGNRSKIADLLGITPRALRYRLASMRKHGIEVFS"
BASE COUNT         4794 a         6744 c         6402 g         5452 t
ORIGIN      
        1 agaataatcc tattaccata aaatattagt tatcacgcag aaaaatcatc ttctcaggga
       61 cgtataagac tgtgctttat gcaaagcact atcaggaaac atttatccct tgctaaggta
      121 aactcaacta tgagtattat tatcaacaac tggcggatgg acccgtcgct taatgcctta
      181 atacattgcg aaacgggtga aacacgtcgt ctcggcgaat accattttat tttgctggaa
      241 aaattagcaa aaaatgccga tgtggtttta tcgcgatctt atttatgtgc cgaagtatgg
      301 aagaaccgca ttgtcggcgg aaacagcctg ccgacggcga tccatgcgtt gcgcgttgct
      361 atcgatgatg atgatggcaa acaacagaac attattaaaa caattccgaa gaaaggttac
      421 ttatgcaata aagagtatgt gtctttgccg gactcatcgc ctgcaaaaaa actgattatt
      481 accgaccaga tacaggaaac agtaccagaa gagatatctt ctacaacacc gcctgtgcct
      541 gttagaaaaa aacataaagt aataatgggg ctagcgttag ccgcggctgt tatatttatt
      601 ggctcgaccg tggggtattc acacttgaaa agtacacctg atgccccgca actggtaaaa
      661 gagtcaatta acagtccaag aataaaaata tttcacctta gttctggaaa agaaaataat
      721 tccgtaccgt tactgtcaca aaccctcgca ccaggaaaag acaaactcga taatttattg
      781 tcagcgcata atatgacaat gacaacctat tataaatatg ttcgtaaccg actggaaagt
      841 gatattgttc tgcgcaacca gtgcaatggt agctggcaat taacttttaa tgtggaaggc
      901 tggcagaaca gcgatattaa tagcgcgatg tatcagaatt tagagaagtt gttaaatact
      961 ttgcagaaat gctgatgagt gccaatatat gccggatgcg acgctaaacg cgtcttatcc
     1021 ggctgataat taccccaata aattcctcac cgtctggctg gaaattcgcg cctgaccatt
     1081 caccgcctgc aataaaacct gatagttatg attcgcgcta tccagcacgc cgccgaggtt
     1141 ttccgacgcc tgcaccgcgt tttccgtttg cgggaagcgc gacatgccgt cgatcaactg
     1201 ccgcgccttt tcctgctgta ccgccatctg cgcacgctgc tgattaatgc tttctaacgt
     1261 ctgagaaatt cccgcaccgc cctgttgcag gctttgagct aaacggtctg cctcagacgg
     1321 ctcggcaaaa gttttcagcg gcataaacgc cgacttatcg ccatcggtgc gcacgcttaa
     1381 ggtacgttcg atatggggcc actgtttttc ggtggtggag aaactgatgc cgtccgcttg
     1441 ctgatgtagc tgcacgccca cgcggcgcag ggcgttggtc agccgcgtct gatactgcgc
     1501 ggcgctgtca tcctcagaca gcatcaccgc cgaaagctga gtctgccgcc cgtcagagac
     1561 gctgaacatg cgcgttcccg gcgtgggatt gtgtaccaga ttcgccagat ccggcgagtg
     1621 aaaggtcacc cgcgcttcgc cctgtaacac cggctgcaac tggcggtcca ccgcaccgcc
     1681 ggaaagcgcg gtgcgtttgt ccagcatctg catcagcgcc gtattctgcg cctgcccgcc
     1741 tttacgctgg ctgtgacgat agtcgagcaa ctgctgctcc agttgcccga gataatgatc
     1801 cgcctgctgc aacgtggtga gctggtcgtt aagctgcacg ctgtaacgct gcgggcgagt
     1861 ggtaatcagc ggcgaggccg gatattcact cgccgtcgcc gggagttttt gccgcaccgc
     1921 ctgagtgggc gcaacggttg acgacgatac cgcagcgcta tgcgcgccgc cggtggcgag
     1981 tgatgaagat gtaagtccga cctgcatact gttcctgaaa gagttagaga acgctgaaca
     2041 aattcaactc actcactttc gagtaagtct tgagggtaac ttccattgcc acttccagtc
     2101 cggtaaaggt gatcgacgcc gggccgtaat cgagatcctg taatgaacca atcagctcat
     2161 cattagaggt ggagatatcg gtctgcgcat cgctcagcat cgacatggta ttttgcgtct
     2221 cgccgagtga cgcaatcgag gcgttgaggt cgtccgaggc ggtgtccacc acatcaacgg
     2281 cattctgaat atcgctctgc acctgcggat ctgccgggtc gacgtccggg ttttgcaact
     2341 cctgggatag ggaattcagg gtgttgagca cgtctaaatt gctgccaaag aaatcactcg
     2401 ccgccacgtt ggtgtccact tccacgccgt tcgacaccgt ggtctggcgg tggtcgctgt
     2461 taccctgata gctgtagctg tcggtgacgc catcgccgtc atcgtccact gccacaatcg
     2521 gcggctgatc gttgatggtg ccgccaaaca cgtagtgccc gtcttcgtcc tgataattga
     2581 gcgccgccac cattgattcg gtgagcgact caatatcctg cccgaggctt gcgagtgaat
     2641 cggcggtatt ggtgccattc gccgcttcca gcaggtcgtc gcgcaccgcc agcaggctgt
     2701 tgttgacgcc atcaaggatc gactcctgct ggcttaagcc cgccgacgcg gagtcgatat
     2761 tgctctgata ctgctcaatc gccgactgct ggcggtttaa ctgggtgatt cgcgtggcgg
     2821 cgatcgggtc atccgacggc tgcaaaatct gcttgccggt tgccatctgc tcgacaacat
     2881 gcgccagatc gccagagaga ttattaaaac tttgcgtcat cgagacgtag gtttgttggg
     2941 tagttactcg cattttcgcg ctcccgctta gctgcacatt tccagcaccg aatcgaaaat
     3001 ctcggccccg gcggaaatga ctttcagatt ggcttcataa atttgttgat aggtgatcag
     3061 gttcaccgcc tcttcgtcca tgctgacgcc gctgacgctg ctctgctggt tttgcgctgc
     3121 ggaatagaca ttggacgcgg catcgacttc cgtctggttt tgctgactgt aaatgccgat
     3181 attgctgatg attgacgagc acgcctgccc gaccgtcacg ctgccgaggt tggctatttc
     3241 cagcggttcg gtggagatat tgatcagcgc ctgcaggttg tcgctgttgc cgctttcgtc
     3301 cggtgaactg gaaaacgcca gctcatcggc ggtgatattc gggttaacgg tcagcgggcc
     3361 atcggcattg ctggcgtcat aaataaacag cggctcgccg gggttaccgt tgagatcgta
     3421 gccctgcgcc agctggttgt tgaccgcatc ggcgaactgc gacgccatgc tgttgatggt
     3481 gtcggtcagc ggcgtcagca catcgttttg ataatcaaac agtgcgccta atgaaccgcc
     3541 ggtgtcggtg gtcatcgtcg aggtggtgcc agcgaaggtc agcgacatgg tcgacgtgcc
     3601 atcggcgttg gtttccagcg cgatggtcga actttgctgc ccactcacca gcggctgacc
     3661 gtttttcagg gtaacgttgt agttgccctg gtcgtcgata ttgacctgca catccatcat
     3721 cccgctcagt tcttccacca tctggtcgcg ggcgtcgtac agcgcggagg cgttatcgcc
     3781 attggcttca gcctgagcga tttgctggtt atagctggcg atgccgctgg tgagcgtatt
     3841 gatttgcgac accatcgcct gctgctggct gatgatttcc gtgctttgcg agtcgatgta
     3901 atccagcgtg ttatcaatac gcaacgacag cgccccggct tcactgatca cctgctcgcg
     3961 cagggcggaa tcatcggggc tggtggttgc ttcgttaagg gcggcgaaga agttatcgaa
     4021 gccgccgctc aggctgctgt tatcgtcgct cagtaccgct tccagttggc tgagatcccc
     4081 ctgctgggtg ctgtaatagc cgtaatcgct ggcggcatac cacacctgat tcacctgata
     4141 ctggttggag acgcggcgaa tgctgtcgac ctgtacgccg ttaccggcgc tgttcggcga
     4201 accgccgctg gcaccgatgg tgctgatctc cgccacctga cgggtgtagc ccgtggtcat
     4261 ggcgttggcg gtgttttgcg ccgtgacgtt aagctccacc tgcgcggttg atgcaccgct
     4321 gtagccgatg ttaatcatgt ccatgttgga tccttaccgg taaatcgtgc cggttaaaag
     4381 cggcgtaaag ttggggattc ttaagttccg taaaccgggt gccgaacgcc ggatgcggcg
     4441 tggacgcctt atccggccta catacgggcg actcgtaggc ctgataagac gcgacagcgt
     4501 cgcatcgtgc accgaacgcc gaagctcatt tatgcttcgg cgacaactgc ttcacaatca
     4561 tcgccgcaat gccaatcccg tgctgctgcg ccagggtgtt acacaactca tcgtcataaa
     4621 acccctgcat catgcgcacc gaatcgctgt taaacgggtt atcttttgaa tcaaacaact
     4681 cgttggtttt ccgcatctct ttcagcatgt tgcgtaaaaa aatcgcctca aactgttcgg
     4741 cggcctgctt gatatcattg gcctgaacct tcggccccat taacgcgtcg ctgccgtcaa
     4801 tcccgccgct ggcgttcact ttcatcagat cacctccagg tcggcatcca gcgcgcccgc
     4861 ctcatgcagc gcctgcaaaa tcgacataat gtcgtccggc gtcgcgccta aactgttcag
     4921 cgcattgacg agcgttttca ggctgctcga ctccggcaaa ctcaccacgc ccggacgggc
     4981 atgattcacc gcgatattgc tctgcggcgt aaccgccgtg cgcccacccg caaaggcatt
     5041 cggctggctg acgttgctgg tttcgttaat cgaaacggtc aggctgccgt gcgacaccgc
     5101 cgccgcgtgt agcgcaacgc catcgcccat taccaccgtg ccggtacgcg agttaaacac
     5161 cacgcgagcg cggattttct ccgcctgcac ctgcacatcg tccagttgcg acataaatgc
     5221 cacccgcgcg ccggggctgg tgggcgcgcg cacggtaacg ttggtggaac tctgcgcggt
     5281 ggcaataccg ccaaacgagg cgttgatcgc cgccgcaatg ttgttggcgt ctttaaacga
     5341 cgggcgtttc aggttgaggg tgatggtatc gcccatctga aaatcgctgg ggatctcgcg
     5401 ctccaccgtt gcgccgttag gaattaaccc ggcggttggc gtattaaccg ttacgctgga
     5461 accgctggcc ccggtggcgt tcatcccgcc caccaccacg ctgccctgtg ccagggcgta
     5521 aacttcccca tcagcgccgt gcagttgggt cagcagcaaa gtgccgccgc gaatactttt
     5581 ggcatcgcca atcgacgaaa cggtgacatc aatggtctgc ccgcgcgagt acatcggcgg
     5641 cagcgtggcg ctcaccgcca ccgcggcgac gtttttcacc ttcgggtcga ttttattcgg
     5701 cagttgcacg ccaaactggc gcagcatatt ggtgatggtc tggttggtga atttgacctg
     5761 gtttttatca ccggtgccgt cgaggccgac caccaggcta tagcccacca gttggttttc
     5821 gcgcacgccc tgtacgttga ccagcgactc cagcgattgc gccagcgcca caccaggcag
     5881 cgccaggctc acggcgataa ctgcggcctt tatccagttt tgcattgcac tctccggcat
     5941 taaatcggga acagaggatg gttaaacagc cgcgtcagcc agcctgcggc gttggcgtcg
     6001 cttaacgcgc cgcgtccggc ataggagatg caggcatcgg cgatccgctg ggaggaaacc
     6061 gagttgtcgt tctggatgtc gtcggcgcgc accaggccgc ttaagcggat gtactcatcg
     6121 ccctggttga gggtgagcca tttctcgccg cgaatttcca ggatgccgtt gggctgcacc
     6181 gcatggacgg aaacggtgat ctccccgcgc aggctgttct gctgctggga agtggcgctg
     6241 ccgtcaaagt cgcggttggc gtcgatagag ccggaaagct tatctttagt gtgactaaaa
     6301 atggtcggtg cgccgatatc gacggtgttg cttttgccga agttggtttt cgcctgtttg
     6361 ctcgactggg tggattcttc gaggatcacc gtcaggatat cgcccacgcg ataagcccgc
     6421 cgatcggcgg tcagcgacca gttgtagcca gtttcaaata caccgccagc acgcccgtct
     6481 gccggaggcg gtgcttcggt tttcggtggc gcaaagtatg cgtcgtcttt tttgaccaga
     6541 atggcctgcg attcacaccc gctaagcagg ggaagcagcg ccaccagcca gagataattt
     6601 ttcacgttct gcctgtttaa atacagtgag ttatgcgctt ttgtaggtcg gataagacgc
     6661 gtcagcgtcg catccgacat ttcggcctta cagcgtctga ctgatgtact gcaacatatc
     6721 gtccgcagcc gacaccattt tggcgttcat ctcgtaagcc cgttgcactg taatcatcgc
     6781 caccatttcg ttgacgatat ctacgttcga gccttcgagg gcattgtcct gcaaagtgcc
     6841 aagcccatct tcgcccggca caccttcggt cggctgaccg ctggcggcgg tttccagata
     6901 caggttgtca ccctcagcgg aaagcccagc cgggttagcg aagttaacca gcgtcagttg
     6961 ccctaactcg gtcacgtcgc tgtcgccagg aagaatcgcc gtcaccgtgc cgtcctcacc
     7021 aaacgcaaca ttcgtcgccc ccgccggaac gtcgatttcc ggctgcaatg gcaagccctg
     7081 tgagttggtc agtacgccat cggcgttcac ctgcaaattg ccgtcgcggg tataggcgat
     7141 atcgccgttg gccttttgca cctgcaaaaa tccctgcccc ataatggcga cattcatcgc
     7201 gttgtcggtg gtttcgacgt tgccttcggt gaaggttttc tgggtgccga caatgcgcac
     7261 gccgctaccg aattgcagcc cggtcggcat gatgttgttc tggtcgagca tcgcgcccgg
     7321 cgcttcctgg gtctggtaga acagatcctg aaacatcacc cggtcacgct tgaagccggt
     7381 ggtgttaacg ttggcgatgt tgttggcaat cgcgctcatt tcagcatcct gcgccgagag
     7441 gccggttttg ctgatccata atgctgcgtt catggtggta aattccgtgt tatttttggg
     7501 ccgcggttgc cggatgcggc gtgaacgcct tatccggcct acgatgtgcg gttacgagcc
     7561 gcgcaataag cggttgcccg cgtcgctgat atcttcggcg gttttcatca tcttgatctg
     7621 tgcttcgaac tggcggttca tggcgatgga cgacatcatt tcgcttaccg ccgagacgtt
     7681 gctgccctcg agaaaaccgc cgctgacttt gatgttttca tcgcgctggg ccggaacgcc
     7741 gtcggcagtc accagcatcc cttcagcatt tttctccagg ttagccaccg ggatatccac
     7801 cagcttcagg cgatcgatat ccatcgtcgc ggtcacatcg ccgtcatcgg gcgtcaccga
     7861 cagcgtgccg tcgctgccaa acgaagcaat ggcgttcggc ggcagaatga tcggcccgtt
     7921 atcccccagc accagattgc cgtcgatggt cagttgcccc tggtcgtcct gctgaatgtt
     7981 gccgttgcgg gtatagactt cgttaccgtt tttatcctgc accgcaatgt aacccgcgcc
     8041 ctgaatcgcc acgtccagcg ggcgttcggt tttctccgca acacccgtgc tgtcgttcac
     8101 gccgctggcc ccttcctgcg ccatatagcg cgtgtcgaag ccgccgcctt tcaccttgtc
     8161 gttggttgcc attgccatat cggcgcgaaa accattggtg ttgacgttcg ccaggttgtt
     8221 ggcgctaatt tgctgctcat acagggtctg agaagcaccg ctcagggcgg tataaatcag
     8281 gcggtccatt acattgcctg gaacagcgcg tcatcgagct gcgtgctggt ggcgataact
     8341 ttggtgttgg cctgatagtt acgctgggcg gtcatcaggt tgaccagttc gctggtgata
     8401 tcgacgttag agctttcgag cacgcccgac gacagcgtac cgcaggtgcc ggagccggga
     8461 acgccaatca gcggggtgcc ggattcgccc gtttgtaccc atgcggtgcc gctcactgcc
     8521 tccaggccgt tttcattcgg gaaagtcgcc agcaccacct gcccctgcaa catgcgctcg
     8581 ccgttgctgt aggtggcgta aactttgcca tcgtcatcaa cctgaacacc gttttgcgtg
     8641 gcggaggcgt agccgttggc ggcgttggtg gtgaccgaaa attcagagcc gtattgcgta
     8701 caggtggagt aatcgacggt taagtcgacg ggcgcggcgg cgtcggtttg aaattcaatg
     8761 gtctgcggcg tggttggaga ggtcagcttc ccggtgttcg gatcgaaggt taacgtcgtc
     8821 gccggaacgc cggtctgctg ctgaccgtcg aaggtgtact gcacttccca ggtgttgtcg
     8881 ctggttttgg tgaagtactg gcataccgaa tgttcgttgc ctaatgagtc atacaccgtg
     8941 gtggtgtagc tgtcggtgta cgaactgctg ttagtcgcgt cgaacggaac ggtggtgcga
     9001 tcgatagctt catcgctggc gttgaagtta gcggtaaagg tcaggctgct gctggcctgc
     9061 gccgggatat tgccggtttt gatctggata tcggtgaccg taccggtttg cagagtgcca
     9121 gtgtcatcga ccggatagcc ctgcaaataa gcgcccgagg cgttgacgat atagccgttt
     9181 ttgtcggttt caaacgatcc ggcgcgggta taggagatat tgcctgcgct gtcacacatg
     9241 acaaaaaatc cgtcgtcgtt aatcgctaaa tcgagcgcat tgccggtgga gaccagcgaa
     9301 ccgccacgcg aaatactttg cgcggtgccc gccacgctga cgcccatcgc ctggcttccg
     9361 gcatacatgg cggaaaactg ggtggtcatc gacttatagc ccaccgttcc ggcgttagcg
     9421 atgttgttac tgatcccgtc cagctgttcg ttaacggcat tcagccccgt tgcggcaatt
     9481 tcataactca ttgtgtcttc ctgttaattg attcttacgc cacatcactg tcggcatcgg
     9541 gtacgccaaa ctggctaatc atggtgaacg gcacttcgcc cacgcctgca acgttaagca
     9601 ccggggaact gccgtcgagc gggatgcgca catcttccac ttcgccggag acttcaatcg
     9661 gcacctcttc ttccccggta ttggtcacca ccgagacgtc gtaatcgccc ggcgggatgc
     9721 cgtaatccgc gggattcacc gagaaatcaa cctggcctgg tccgacggac gaactgcctt
     9781 ctgggatcag cgagacggtg tagtcatcgc ctgcggcatc ggtaaagtgg agatcggcgg
     9841 tggtacaggc gtcgtccagc gtagcgcgac cgttaatggt ctggtcgctc acctgcaagg
     9901 cggtggtctg caccataatg tcgtcgccca ccaggttacc gagcgccatc acctgaatgt
     9961 tgctcatcag caccgcgttg gtgttggcct gcacgctcat ctcttccatc atcgccacct
    10021 gcgccatcga cgacagctgg tcgatgtatt cggtagcgtc ggtcgggtcg gtcgggtcct
    10081 ggttttggat ctcggcgaca aacagcgtca ggaaggtatc gacaggcgag ctatcgctgc
    10141 tgctttgcgt gtcgctggcc gccacattat tgctttccgc cgtggcggtc ggctgcggcg
    10201 attgcgcatt cagcgccagg gtattcatca ggcttctccc agtttcagca aactttgctg
    10261 catacttttc acgttgttga gcacatcgac gttggtttcg aaatcgcgcg aggccgacat
    10321 catgtccgcc atctgctcca ccacgttgac gtcgggatac cagacgtcac cgttggcgtc
    10381 ggcgagcgga gaatgcggtt catagcgttt caccgccccg ccggtttgca gcacgtcctg
    10441 tacctgcacg ctggcaccgt caatggcatg acgatgtgta cccgccagca ggctgtggtg
    10501 atacaccgcg gcaaacaccg ggctgcgcgc tttatacgcc tgcgcctcgc tgctggcggg
    10561 cgcattggca ttcgccaggt tactggcaac ggtattcaga cgtagcgttt gcgccgtcat
    10621 cgccgagccg gaaatctgat agatatcagt aaatgacatg cttatttccc ttcaatggcc
    10681 tctctgatgc cgctgatttg cagattcaga aaggtcaaac tgctttgata atccatacta
    10741 ttttgtgaaa accgcgcctg ctcgctgttc agttccacgg tgttgccatc ttccgacggc
    10801 tgcatcggca cgcggtactg gacatccacc gccgcgttat ttgcccgttg catctccctg
    10861 gcaaaatcaa tatctttagc cttgaaattt ggcgtatcga cgttcgccag attcgcagtc
    10921 agtagctcgg tcctctcaag acgtaacttc actgcctgcg gatgtactcc caatgcctgc
    10981 tgaaaactga tccccatttc ctgcatcact cctgcttcaa tccgcatacg ctaagcaatc
    11041 gccatgccat tttttaagtc actgatgaga aagggtttat gagttttcaa ttttgcaggc
    11101 ggaagtttta cttcctcctg gcgctggcct taccgggcta cgccgccgtc catcccgtgc
    11161 aacacagcgc gcgggaacaa gtcaacgccc aggtgctgaa tgccgcgagc caaaaaattg
    11221 aatcgctggc gcagcagcgg cagtggcatg actatcgcta cacctttaag atctacattc
    11281 cctcgcagat tgcgaccgcc gcgccctgta ctaaaacacc cggcgtcacg ctgacttcgc
    11341 ctgcggaaat cgccctcaac cggatgaact tcaccgttag ttgcccgcaa agctggcaga
    11401 tgaacgtggc ggtgcgcccg gatgtactgg tgccggtggt aatgacgaag tcactggttg
    11461 cccgcgatat gccgctcacc gcgaatgatg tggagcttaa gccctacaac gtcagcgccc
    11521 agcgccgcga tgtgttgatg gagcttgatg acgcgattgg tttaagcagt aagcacgcat
    11581 tgcaaccagg cagacccatc accaaagaag aactggtgtc tccggtgctg gtcgggcgcg
    11641 atcagccggt gatgattgtt taccagagcg cggggatcac cgcctcgatg cccggcgtgg
    11701 cgctgaaaaa tggtcgtaag ggcgagatgg tgaaagtgcg caatgccagc agccagcggg
    11761 tcatcagcgc gatggtggcg gaaagcggcg tggtgacaac ggtaagcgcg gagtaatttg
    11821 cgcaggcgat taaagttttg ccgcctgtta accgctttac cgcagtaacc cacccaaata
    11881 ccgagacgag gttgcgatga aaatcacccc gactatgcct ggcaatcgcc cgacagccac
    11941 aacggcaggc tccagccagc cgcgcaaaac cacggtgacc accaccacga cgctgagcgc
    12001 cgacgacatt actcaggcgg gattgcagtc tgcccagcaa acgctgaacg accagcaaga
    12061 gagcgatatc gacagcaaca aagtggcgca agtgcaggcg atgctggcgt ccggcagccc
    12121 gcaagtggac actgaacagt tagccagcga catgctgagt tttttccaga actaagaggc
    12181 cgatgaagtg agtatccggt tacaacaggt gaaggctttg ttgcagggca ttcgtgacga
    12241 tgacgcgctg tatgattccc tgcgcgaact tctccagcgt cagcggatct gcatgattcg
    12301 ccgcgccagc gaagagttgc tggcggtaaa cgatgagata acccatcact atgagcagtt
    12361 gcatggacat agccatcagc ggcatagcct gctgaaaatg ctcggtgtta gcgtaaatcg
    12421 tgacggtctg gcgcaggttt tcacctggct tccggctgtt caaaaagcgg ctgcacaaca
    12481 gttgtggcaa cgccttgagc aaaaagcgga acgctgcaaa gcctacaacg acaaaaacgg
    12541 cgagctgttg attcgccagt atgaatttat tcagtcgttt ttaggcagcg aagcagattt
    12601 tctctatcag gaataacgcg aataccttcc ccatagcgga aatggatttt ccgcctgatt
    12661 tccaatccct tgtttttaat tacaaaaatc cccggcacgc ttattgcata tcctgcacag
    12721 cattaaagac tggaagccac ctatgagttt tattgaatgt tacgaaccgg aatacgtgcg
    12781 caaattttta gcgcgtcccc ccggttcgcc tttatacgtc cgcaaggtgt ggaaaaacga
    12841 gattcgcctg agcctgtcat tgaccgatat tgccagctgc gaagcggtgc tcaacgatgt
    12901 cagggccttt gatttacagc tcacctaccg ccctgtggaa gataattccg cagagctttc
    12961 cgcgcgcgat gcgatagtaa accaggtgat tttgagtacc ctcacgctcc gtgatttaac
    13021 cccggagctt tccctgtatg ctgtcgggat tttgctctcc tgcgccagca aaatgccggg
    13081 cagagacgga gacatcctgg cccgtttgac gacgctgcca caggcgctgg ccgatcatgc
    13141 gaaaaaaggt acgctacagg cacagtacgc ccagctcccg cccgtgccac agctggcgcg
    13201 ccagttgatg acgctgctcg gcggttgtgc gtttgactgg tcgattttgc cggaaagccc
    13261 gcgcaaagcc tcgctgccgt tgcaggttac tttactgacg ctgcatgacg ccaacagcga
    13321 agccctgtta caacagcagc ttcagacgca gtggcaaacc acctggcaac agcattttgc
    13381 caccgcgccc tggatgatgc gtaactggct gatttaccgc gtttaccacg atatgattgg
    13441 gcagactgac ggggcggatt atttcccgct ggtgtgcgat ttttatctcc tccgcacgtt
    13501 aatcagttta tggacgctgg atggttctcc gctgcggcag gaggatattt tcgccctctt
    13561 tgccatgttt gaacgctggc gagccagtga aaatgcgctg ttggttcgcc agcaaattca
    13621 gtcgttatgt gccgccgacc cgctgctttc agcgttttcg ctgctaacgt gagtcgctct
    13681 gcgcgcgctt tgcccctcac cctaaccctc tccccaaagg ggcgagggga ccgatcgagc
    13741 acggtgttaa aatcggcgct gattttctcc ctctccctgt gggagagggt cggggtgagg
    13801 gcatcagcgc gcacgttcat cacattcaca cattaactca cccaaacacc atgccaacac
    13861 caaccatcat caaaacactc gccacccacc gcgaaaaacc gtttgtcagc gtcgtcacgc
    13921 caacctggaa ccgcggcgca ttcctgccgt acctgctcta tatgtaccgc tatcaggatt
    13981 acccggcaga ccgccgcgaa ctcatcatcc tcgacgattc cccacaaagc catcaacaca
    14041 tcatcgaccg cctgaccaat ggcacgccgg aagcctttaa catccgctac atccatcacc
    14101 cggaaaaact gccgctgggc aaaaaacgca acatgctcaa cgaactggcg cgcggcgaat
    14161 acatcctctg catggatgat gacgactact atccggcaga taaaatctct tacaccatcg
    14221 ctatgatgca aaaacaccgg gcgctcatct ccggttccga ccagatcccc atctggtaca
    14281 gccatatcaa ccgtatcttt caaagccgca gctttggccc acataatatc ctcaacggca
    14341 ccttttgcta tcaccgtaac tacctgaaaa agcaccgtta cgacgatgat tgcaatttag
    14401 gcgaagagaa aagtttcacc gacaatttca gcgttaaccc gctgcaactg cccggcgagc
    14461 gcacgatttt gtgtatctcg catagccaca acacctttga taaagacttt attatcgggg
    14521 ccagcacgcc ggtaaacacg gcgcttacgg atatcgtccg cgatccgctg ctgcgcaatg
    14581 cttatctgag tctgcataac gccactcatc accaggcgat caaccatcag gcgatcgatc
    14641 aggtggtcgt cgtgaatctg gataaacgcc cggatcgctt acaacagatc cgtgaagaac
    14701 tggcattgct gcatattcca cccgagaaga tcacccgcct tgccgccagt gaagatgaaa
    14761 acggtcaacg gggccgccgt cagtcgcact tgcaggcgct gcgtctggcg caacagcacg
    14821 gctggcaaaa ctatctgtta ctggaagatg acgcggttat tctcaagcag gagaaacata
    14881 ttcaggtact gaacacgctg ctggcgagcc tggcgaaaat tccctggcag gtgatgatcc
    14941 tcggcggcga aatttcgcaa ggaacgatgc taaaaagttt gcctgggctg gtgcacgcgc
    15001 gggactgccg caaagtgtgc gcttatctgg tgaacagccg ctactacccg caactggcgc
    15061 agcagatgag caacgatgag cactcgctgg aagactgctg gcaaccgctg ctacgcgccg
    15121 acaaatggct ggcctgttac ccgagccttt gttatcagcg accgggcttt agcgatatcc
    15181 agaaaaaaac gactgataat attgctcgct atttcaataa gttacccgta accaccaagc
    15241 catccgcgtt acccatcgcc gacaccatcg gcttttttat ggaaacctca ttccactacg
    15301 cgctgtaccg cccaatcatc accgccctac aggcgcaagg gctgagctgc acgctggtga
    15361 tcaacgaccg cgtgtttaaa ccgtttctgg atgagatgct ggaaacactg aaaaacatcg
    15421 acgatccgca gcttaaaggt atgcgtctgt cagaaatgca gacacatggt cagcgggtga
    15481 aatgcctggt cagcccctac cacacgcctg cgctcaatgg cctggcggcg gtgaacatcc
    15541 gcgctatgta cggcttagcc aaagagacgt ggaaccacgc cgactggaat cgtttttacc
    15601 aaagcatttt gtgttatagc cactacagtc agcaggcgct tgcgcatttc ggcagcgcaa
    15661 aagtggtcgg caacccgcgc ttcgacgcct ggcataacgg cacgttcgat cgcgcgttgc
    15721 cggaaaacat ccaatcagat taccgtaaac ctacggtgct ctatgcccca acgtttggcg
    15781 cattaagctc cctgccccac tgggctgaaa aattgggccg cttgagcggt gatgtgaacc
    15841 tgatttgcaa actgcaccat ggcacctgct cacgcccgga agaagcagca tcgctggccc
    15901 tggtgcgcag gcatttaaaa cagcgcaccg actccgcccg ccacacgctg gcgctgctgg
    15961 caaaagccga ttacgtgctg accgataaca gcgggtttat tttcgatgct atccacgttg
    16021 ataagcgggt gatcctgctc gactttccgg gcatgaatga tttgctcgac ggtgagaaaa
    16081 gttattccac ggcagagagc gccgaccagc gaatccgcga aatattgccg gttgctcatg
    16141 atgttgcaga actgcgttat ttgttgtcag aggcgtttga ctgggggtca gtgcaggcgc
    16201 ggcttacgga gattcgccat cactattgcg atgcgtttat ggatggcaag gcgggagaac
    16261 gggcggcgat tgtgattgtg gaggcgctgg ggtaaaccgc gcaactgcct gatgcgcttc
    16321 tcttatcagg cctacggatc gatcacgtcc tgtaggccgg ataaggcgtt tacgccgcat
    16381 ccggcaagaa aatacatctc tgttttagta ctttataaaa gccctctaat cacctcaata
    16441 atccccgtcg tcgacaccga cggcgtgcgg gggaaataga ccacctcaca aatatacgca
    16501 aagctgtcaa acttccctgc ccagtcatcc cccatcacta aaatatccgc actgtacccg
    16561 cgcaaatact ccgctttctg ctctagcgat tcttccagaa atacgccatc cacgcacgcc
    16621 agcccggcga tgatcgccat gcggtcatcc tgatggtaaa ccggtgccct gccctttttg
    16681 gcgatgttca gggcgtcgga cgatacgccg accagcagcc gctcgcccaa tgcgcgggcg
    16741 cgttgtaaaa ggcgcagatg accgacatga aacacatcga aggtgccgaa ggtgatgacc
    16801 gttttcatgg ctactccgct aatttgtgcc gcagccagca ctgcacggcg atgtcgtcca
    16861 ccgctttttg cgctttcgcc gtggcgcttt cttgttgctc gcggcgtttg tcgtccagcg
    16921 tcagggcgac tactttctct tcgcacgccg ccgccgtgag gtttttctgc attcgcgtgg
    16981 ctttgatatt ggccagcgtt ttttcctgct cctgccaggc gattaccttg cgcagggtgc
    17041 ctttgtagcc gctgacgttt ttcagcgatt ccaccgacac cgccgcaccc gcagatgttt
    17101 tctcgacaag gtagcctaac gccttgatat tgttgtcata acccgcgcaa atctgttttt
    17161 gctttgccag ctcgacggtt ttgtctttga ccgatttatc ccgcaggcgc tgcaactgcg
    17221 tcagggtgtc gatgatttgt cgcatagtta ctgttctgcc tttttgacac caggaaagag
    17281 aatttgcagg cggctttgca ccagttcgag ggaggcgggt tcgcgcatct cctggcgcaa
    17341 aaatcgctcg atggccggga acatttttac cgctttgtcg acgctggcgt cggcacctgc
    17401 cacataaccg ccgagcggga tcagaggttt gatctccata taggcggcat agttttgctt
    17461 taacgcgcgg gcggattgct gatgttccag gctggtgacc tggttcatgc agcggctgat
    17521 cgactggccg atatcaatgg ccggatagtg ccccgcttcc gccagtttgc gggttaatac
    17581 gatatgcccg tccagcaccg cgcgcgcgca gtcgacaatc ggatcttgct ggtcgtcacc
    17641 ttccgccagc acggtgtaga tggcggtcat cgatcccgcg ctttcgctgt tgcctgcgct
    17701 ttcgaccagt ttggggatca tgccgaatgc cgatggcgga taacctttgg tggcgggcgg
    17761 ttcgccgagc gagagggcga tttcccgctg cgccatcgcg tagcgggtca gggaatccac
    17821 cagcagtaag acgtgatgcc cgcggtcgcg aaaccaggcg gcgatggagt ggcacagttc
    17881 ggtggctttc agacgcatca gcggcgattc atcggcaggc gcgacgacga cgatagactt
    17941 tgccagcccg tccgcgccca gcgagtggtc gataaactct ttcacttcgc gcccgcgttc
    18001 cccaatcagc ccaaccacca cgatatcggc tttggtctgg cgggtgatca tccccagcag
    18061 cacgcttttc ccgacgccgc tgcccgccat tagtccgacg cgctggcctt tgccgatggt
    18121 cagcaggccg ttgatcgctt tcacgcccac gtccagcggt tcgtctaccg aacgacgagt
    18181 taagggatta accgagggcg gcaacggcgg cagaaggtcg ttgccgttca ggcggccttt
    18241 gccatccagc ggttcgccga ggccattcac cacgcggcct aaccagcttt cgcctatcag
    18301 gatgtcgtgg gctttttctt ccgggaagac gcgtgcgcct gccatcaggc cgaccggctg
    18361 tttaaacggc attaaatagg tgatgtcgcg attaaagccc acggcctgcg cgtcgatcag
    18421 cgtgtgattg gcactttcga tgcggcatag ctgcccggtc attagcgggc agccgacact
    18481 ctccagcagg atgccgttaa ggcgcaccag ccgcccggcg acgcgggcca gcgggatcga
    18541 ctccagcgag cgcagggcgt tatcgaacgc agaaaaatca ctcaccctgc gactccggcg
    18601 tgagtgtttc tttcagcgcg gtcatgcatt gttcaaggcg atgctcgcag ccgatatcca
    18661 gttcggattt gtcggtgatc acccggcatt cgcccggtgg caaatccggc gaaggcgtca
    18721 gcccccattc gctgaccttt tccggcaccg cgtcgcgcag gcggttaaac tcttccgtgc
    18781 tcagcattac ttgcagggtt tccggcatcg ccgggaaggc cgccagcgcc tcttccacca
    18841 gcgccagcaa ctgcgtgggt tgcagcgcca gttcacaacg gatcacctgg cgggtgactt
    18901 tttccaccag ttgcagcaaa tcttcccgct gtttacgttc gatatgagcg aggaagtcgt
    18961 tcactttacc ggtgatggct tccagcggct gcgccgcctc aacaaactgt ttgcgccctt
    19021 cctgctgccc ggcgaggctg ccttcggtgt aaccctggcg tcgcccctcg gcaaaacctt
    19081 gctgatgacc ttcgctaaag ccctgctcct ggccttcggt cattccttga gcaaaccctt
    19141 tttgcagccc ttcctgaaat ccgtccatca actggcgctg gtaatcggcg gcgctaatac
    19201 tggtagtgag cggttctccg gtcaccgcac gctggcgcgg cgggaaacgg tgcaggcgat
    19261 agcgcccgcg cagtgtttca atggtcatgg ttactccgcc gtctgttccg caaagagctg
    19321 tagctgaatt tcgcccgcct cttccagttc acgggcgatg cccatgattt cgcggcggat
    19381 ctgttcgata cggctgaccg gcaccggccc cagacgcgcg gtgatggctt ccagttgctg
    19441 cgcctgccgt tttggcatta ccgcgtaaat cgaacggcgc agcagggctt cggtgccttt
    19501 cagcgccacc gcccagtctt ccatcggcac ttcgtcaagc agacgtcgac gcacctcttc
    19561 gttctggcgg ctgaggataa agaagtcgta catctcatcc tgaagctgct ccagcacctc
    19621 ttcgtcgcgc tcgcgcatct ggtcgaggat cacctgctgg ttgccctgga agcggttgac
    19681 gatatccgcc gcctgtttga tgcctttcac ttttgagccg tgttccgaaa gcaccgacag
    19741 gccgcgttcg atcagcctgt ccagttcatc caccacgtcg cggttgacgt cgttgagttt
    19801 cgccacccgg tagagaattt cgttttgcac cgattcgttg agatacgaga gcaccgctgc
    19861 cgagatttcc ggcgtcagaa acgcgaggaa caccgcctgt aattgcagat gttcttcgga
    19921 gatcagcatc gccagctggc gtggttccac ccattgcagg cgcgccatcc gcgagcggat
    19981 ctcatcgccg tagatgccgt tgatcacact gctggcgatc tcggtgccga gggctttgtt
    20041 gagaataccc tgcaacatgg atcgcgatgc gccgttgatc ccgctctgtt cgcggaactc
    20101 atcaaagaag ttattgatca ctttgcgcgc catgctggtt ttcacgccgg aaagccgtgc
    20161 catgttttcg ctcaggcgca ccacttcttc gcggctgagt ttttgcatca ccgtcgccgc
    20221 cgcctcttcg cccagacaca gtagcaggat cgccgcctgt tccaggtagc tgttgctctt
    20281 atttgttgtc aattcgctca ttgctggtga tccattgtct gagaacttcg gctacgcgat
    20341 cggtgtcgct catggccagt ttttgcagga actcgagttt tacttccagg ccggaactct
    20401 gagacggcag gctgtcatcg ccgggcagcg acggcagatc gacgtttttg cgctcctctt
    20461 ccggcatggc aatccacggt tcgctgacgg cttccgattc tggtgtgata atcggtgcac
    20521 gtaccgccgt cagccgtttc attaccgggc gcaccacgaa gaacagcaac aacagcgcca
    20581 gcaggccgca gccaatcagg cgcacccagg ccagcacgct gtcatctttc cacagcggaa
    20641 taattggttc gaccggcacc gcctgcggca caaagttaag cagtgaaagc gtcaggttat
    20701 ccccacgttg cgcgtcaata ccggcggcat tgttcagcaa cgcggtaagc tgcgcggtct
    20761 gctccggctt ccagtttttc agcgccggag cgttttgatt gagcaccacc gccacattga
    20821 ggcgtttcac cgcaaagccg ggatgctgaa tatgctggac gctacggtcg taagagtaat
    20881 cgcgcttgct ttcgctgtgt ttggataacg cttcaggcga gcgattttct tccgtaccgt
    20941 tagtcatttg attcgccgca accggcggac ggttgctgag agagccgggc acacccatcg
    21001 ccacctgatt ggtgtcgctg tccagcacgc tctcttcgcg gtttattttc ggcgcgtcgc
    21061 cgtagtgttc ctgagtttcg tcgatggtgc tgaggtcgag atccggcatc acgctgacac
    21121 ggtaattccc gctgccgacc agcgaatcca gcacgttcgc cacgctggcg tgggttttgt
    21181 cctgaatgtc tttgaggatc tgatcgcgtt tacgggtagc ggcagagacc gcctcgcccg
    21241 cgccgatgcc gtcggtcagc agatttcccg cctgatcaac cacgctaact ttgctggcgt
    21301 gcagccccgg aatgctgccg gaaaccagat gcacaatggc gttcacctgg tccatattca
    21361 gcttcgcgcc gtagtgcaga cgcaccacca ccgaggcgct gttttgcggc tcatcgctga
    21421 ccacaaacga gctttcttcg ttgagcgcca gatgcacccg cgcgctctcc accgcgtcca
    21481 gcgacataat gctctgcgcc agctcccctt cgaggctgcg tttatagcgc acgttctgca
    21541 cgaactggct gctgccaagc acttcgtctt tgtccatcag ctcgtagccg ctgggcaaaa
    21601 tcgcctgcac gcctttggcg gcaagggtca tgcgcgtttt cgacagcgcc tcttccggca
    21661 ccagtatctg cccgctttgc gggtcaatgc ggtaggtgag cttttcgccg tccagcacgg
    21721 tgacaatctg cgacacgggc agattttcct ggcgtccgta gagcgagacg tagccgcgat
    21781 tgccggtcca cagcacgcta acgataatcg ccgtcgccgc cacggcaagc cccacacccg
    21841 ccatcagcgc ccagcgtttg ttatctgcga gacgcagtcg aaacgccgga aacgccaggg
    21901 ttaacttctt tatttgtgca ttcatagggt tagatcgaca tactcatcag atcgttaaat
    21961 ccggtggcga ttttgttgcg cacctgcacc agcgcggaga aagagagcga cgcctgctgg
    22021 ctggcgatca tcgcacctgc cagatcatcg cttttgccca tgtcgatagc cgtttgctgc
    22081 tgctccgcca cctgctggaa ctggtcaacg tgaccgagcg cgccctgcat aatgcggtga
    22141 aacgaaacgt ccgggtcggt ggaactgaag gtcatggcgg gcagcaccgg cgcctgggcg
    22201 ttggcctgca tccgctgcac gtcctgcatg atctgcgcct gcatggtgct gttgattgtg
    22261 gtaatgctca tttttaactt ccgggtcagg agaaaacttc gataccgtgc ttgcgcatgg
    22321 aggccagtcg atagcgcagt gcgcgcgggg taatacctaa caggtcggcg attttgctgc
    22381 ggttgccctg atatttacgc atcaggtcag cgatatattg atactgggcg cttcgtccgt
    22441 gctggcctaa atcgctggtt tcggcaatgt gtacggcggg ctgagcaggc cattgcagtt
    22501 cgctgtggtc ggcaatgtca ttttccggca ggcctaaggc gtccgggtaa atcacgccgt
    22561 cgcgatttaa aatcattccc cgctgaatgg cgttctccag ctggcgcaca ttgccgggcc
    22621 agcggtaatt cagcatcgca cggcgggtag attctgaaag ggtgatattt ttcaccagca
    22681 ccgtggagta tttttttata aatgactctg ccagcggaat aatatcgttc agacgttcac
    22741 gcagcggcgg catggtaata ggaataaccg ccagacgata atagagatct tcacgaaaac
    22801 gcccggcggc gacttcctgc tcaaggtttt tattggtgca ggcaatcagg cgaaaattga
    22861 gtttaatttg tcggttgctg cctaatcgct caacctgttg ttcctgcaat acgcgtaata
    22921 ttttggcctg taacgccagc ggcatatcgc caatttcatc gagtaataag gtgccgttat
    22981 tcgccagttc catttttcct ggcacactgg caattgcgcc ggtaaatgcg cctttgtcgt
    23041 agccaaataa ggttgcttcc agcatgtttt ccgggatcgc agcgcagttc acgccgatat
    23101 agggcgcgtt ttcattttca ccaaaggcga ctgtgtgaat atatttcgcc acgcactctt
    23161 tgcccgcgcc ggtttcgccc tgaatgagca ccggcacgtt aaatgcggcg acacgctttg
    23221 ccagagtaaa agcgttaatg ctggaagcgg cagttgcaat aagttcagac atagggtgaa
    23281 cgatataagt agcgacaata atcaggatgt tctgaagcgt atcaccggcg ggattaacaa
    23341 gttcgaatta aacgataata aaaacattat ttgtttataa acaatgattt at
//
LOCUS       DAZKHF020000051        23362 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2201S31241
            SAMN43267800-rid25106413.denovo.051, whole genome shotgun sequence.
ACCESSION   DAZKHF020000051 DAZKHF020000000
VERSION     DAZKHF020000051.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267800
            Sequence Read Archive: SRR30307263
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 23362)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23362)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 23362)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350581.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350581.2
            Genome Coverage       :: 151x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:21:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,652
            CDSs (total)                      :: 5,557
            Genes (coding)                    :: 5,320
            CDSs (with protein)               :: 5,320
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 237
            CDSs (without protein)            :: 237
            Pseudo Genes (ambiguous residues) :: 0 of 237
            Pseudo Genes (frameshifted)       :: 102 of 237
            Pseudo Genes (incomplete)         :: 130 of 237
            Pseudo Genes (internal stop)      :: 52 of 237
            Pseudo Genes (multiple problems)  :: 41 of 237
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23362
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267800-rid25106413.denovo.051"
                     /serotype="O 26:H11"
                     /isolate="2201S31241"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-01"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            206..1150
                     /gene="leuO"
                     /locus_tag="ACHOSC_RS08110"
                     /old_locus_tag="pgaptmp_001622"
     CDS             206..1150
                     /gene="leuO"
                     /locus_tag="ACHOSC_RS08110"
                     /old_locus_tag="pgaptmp_001622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414618.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator LeuO"
                     /protein_id="HGU4547584.1"
                     /translation="MPEVQTDHSETAELSKPQLRMVDLNLLTVFDAVMQEQNITRAAH
                     VLGMSQPAVSNAVARLKVMFNDELFVRYGRGIQPTARAFQLFGSVRQALQLVQNELPG
                     SGFEPASSERVFHLCVCSPLDSILTSQIYNHIEQIAPNIHVMFKSSLNQNTEHQLRYQ
                     ETEFVISYEDFHRPEFTSVPLFKDEMVLVASKNHPTIKGPLLKHDVYNEQHAAVSLDR
                     FASFSQPWYDTVDKQASIAYQGMAMMSVLSVVSQTHLVAIAPRWLAEEFAESLELQVL
                     PLPLKQNSRTCYLSWHEAAGRDKGHQWMEEQLVSICKR"
     gene            complement(1305..1349)
                     /locus_tag="ACHOSC_RS08115"
                     /old_locus_tag="pgaptmp_001623"
     CDS             complement(1305..1349)
                     /locus_tag="ACHOSC_RS08115"
                     /old_locus_tag="pgaptmp_001623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4547585.1"
                     /translation="MKRNDKILHYEGLN"
     gene            1468..3192
                     /gene="ilvI"
                     /locus_tag="ACHOSC_RS08120"
                     /old_locus_tag="pgaptmp_001624"
     CDS             1468..3192
                     /gene="ilvI"
                     /locus_tag="ACHOSC_RS08120"
                     /old_locus_tag="pgaptmp_001624"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020079613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0003984 - acetolactate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0009082 - branched-chain amino acid
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase 3 large subunit"
                     /protein_id="HGU4547586.1"
                     /translation="MEMLSGAEMVVRSLIDQGVKQVFGYPGGAVLDIYDALHTVGGID
                     HVLVRHEQAAVHMADGLARATGEVGVVLVTSGPGATNAITGIATAYMDSIPLVVLSGQ
                     VATSLIGYDAFQECDMVGISRPVVKHSFLVKQTEDIPQVLKKAFWLAASGRPGPVVVD
                     LPKDILNPANKLPYVWPESVSMRSYNPTTTGHKGQIKRALQTLVAAKKPVVYVGGGAI
                     MAGCHQQLKETVEALNLPVVSSLMGLGAFPATHRQALGMLGMHGTYEANMTMHNADVI
                     FAVGVRFDDRTTNNLAKYCPNATVLHIDIDPTSISKTVTADIPIVGDARQVLEQMLEL
                     LSQESAHQPLDEIRDWWQQIEQWRARQCLKYDTHSEKIKPQAVIETLWRLTKGDAYVT
                     SDVGQHQMFAALYYPFDKPRRWINSGGLGTMGFGLPAALGVKMALPEETVVCVTGDGS
                     IQMNIQELSTALQYELPVLVVNLNNRYLGMVKQWQDMIYSGRHSQSYMQSLPDFVRLA
                     EAYGHVGIQISHPHELESKLSEALEQVCNNRLVFVDVTVDGSEHVYPMQIRGGGMDEM
                     WLSKTERT"
     gene            3195..3686
                     /gene="ilvN"
                     /locus_tag="ACHOSC_RS08125"
                     /old_locus_tag="pgaptmp_001625"
     CDS             3195..3686
                     /gene="ilvN"
                     /locus_tag="ACHOSC_RS08125"
                     /old_locus_tag="pgaptmp_001625"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001563086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:1990610 - acetolactate synthase regulator
                     activity [Evidence IEA];
                     GO_process: GO:0009082 - branched-chain amino acid
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase small subunit"
                     /protein_id="HGU4547587.1"
                     /translation="MRRILSVLLENESGALSRVIGLFSQRGYNIESLTVAPTDDPTLS
                     RMTIQTVGDEKVLEQIEKQLHKLVDVLRVSELGQGAHVEREIMLVKIQASGYGRDEVK
                     RNTEIFRGQIIDVTPSLYTVQLAGTSDKLDAFLASIRDVAKIVEVARSGVVGLSRGDK
                     IMR"
     gene            3698..3784
                     /locus_tag="ACHOSC_RS08130"
                     /old_locus_tag="pgaptmp_001626"
     CDS             3698..3784
                     /locus_tag="ACHOSC_RS08130"
                     /old_locus_tag="pgaptmp_001626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fruR/shl operon leader peptide"
                     /protein_id="HGU4547588.1"
                     /translation="MRNLQPNMSRWAFFAKSVGTWNKSSCRS"
     gene            3866..4870
                     /gene="cra"
                     /locus_tag="ACHOSC_RS08135"
                     /old_locus_tag="pgaptmp_001627"
     CDS             3866..4870
                     /gene="cra"
                     /locus_tag="ACHOSC_RS08135"
                     /old_locus_tag="pgaptmp_001627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0009750 - response to fructose [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="catabolite repressor/activator"
                     /protein_id="HGU4547589.1"
                     /translation="MKLDEIARLAGVSRTTASYVINGKAKQYRVSDKTVEKVMAVVRE
                     HNYHPNAVAAGLRAGRTRSIGLVIPDLENTSYTRIANYLERQARQRGYQLLIACSEDQ
                     PDNEMRCIEHLLQRQVDAIIVSTSLPPEHPFYQRWANDPFPIVALDRALDREHFTSVV
                     GADQDDAEMLAEELRKFPAETVLYLGALPELSVSFLREQGFRTAWKDDPREVHFLYAN
                     SYEREAAAQLFEKWLETHPMPQALFTTSFALLQGVMDVTLRRDGKLPSDLAIATFGDN
                     ELLDFLQCPVLAVAQRHRDVAERVLEIVLASLDEPRKPKPGLTRIKRNLYRRGVLSRS
                     "
     gene            5472..5930
                     /gene="mraZ"
                     /locus_tag="ACHOSC_RS08140"
                     /old_locus_tag="pgaptmp_001628"
     CDS             5472..5930
                     /gene="mraZ"
                     /locus_tag="ACHOSC_RS08140"
                     /old_locus_tag="pgaptmp_001628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020325403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="division/cell wall cluster transcriptional
                     repressor MraZ"
                     /protein_id="HGU4547590.1"
                     /translation="MFRGATLVNLDSKGRLSVPTRYREQLLENAAGQMVCTIDIHHPC
                     LLLYPLPEWEIIEQKLSRLSSMNPVERRVQRLLLGHASECQMDGAGRLLIAPVLRQHA
                     GLTKEVMLVGQFNKFELWDETTWHQQVKEDIDAEQLATGDLSERLQDLSL"
     gene            5932..6873
                     /gene="rsmH"
                     /locus_tag="ACHOSC_RS08145"
                     /old_locus_tag="pgaptmp_001629"
     CDS             5932..6873
                     /gene="rsmH"
                     /locus_tag="ACHOSC_RS08145"
                     /old_locus_tag="pgaptmp_001629"
                     /EC_number="2.1.1.199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016434 - rRNA (cytosine)
                     methyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(1402)-N(4))-methyltransferase
                     RsmH"
                     /protein_id="HGU4547591.1"
                     /translation="MMENYKHTTVLLDEAVNGLNIRPDGIYIDGTFGRGGHSRLILSQ
                     LGEEGRLLAIDRDPQAIAVAKTIDDPRFSIIHGPFSALGEYVAERDLIGKIDGILLDL
                     GVSSPQLDDAERGFSFMRDGPLDMRMDPTRGQSAAEWLQTAEEADIAWVLKTYGEERF
                     AKRIARAIVERNREQPMTRTKELAEVVAAATPVKDKFKHPATRTFQAVRIWVNSELEE
                     IEQALKSSLNVLAPGGRLSIISFHSLEDRIVKRFMRENSRGPQVPAGLPMTEEQLKKL
                     GGRQLRALGKLMPGEEEVAENPRARSSVLRIAERTNA"
     gene            6870..7235
                     /gene="ftsL"
                     /locus_tag="ACHOSC_RS08150"
                     /old_locus_tag="pgaptmp_001630"
     CDS             6870..7235
                     /gene="ftsL"
                     /locus_tag="ACHOSC_RS08150"
                     /old_locus_tag="pgaptmp_001630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000625658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsL"
                     /protein_id="HGU4547592.1"
                     /translation="MISRVTEALSKVKGSMGSHERHALPGVIGDDLLRFGKLPLCLFI
                     CIILTAVTVVTTAHHTRLLTAQREQLVLERDALDIEWRNLILEENALGDHSRVERIAT
                     EKLQMQHVDPSQENIVVQK"
     gene            7251..9017
                     /gene="ftsI"
                     /locus_tag="ACHOSC_RS08155"
                     /old_locus_tag="pgaptmp_001631"
     CDS             7251..9017
                     /gene="ftsI"
                     /locus_tag="ACHOSC_RS08155"
                     /old_locus_tag="pgaptmp_001631"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000642204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan glycosyltransferase FtsI"
                     /protein_id="HGU4547593.1"
                     /translation="MKAAAKTQKPKRQEEHANFISWRFALLCGCILLALAFLLGRVAW
                     LQVISPDMLVKEGDMRSLRVQQVSTSRGMITDRSGRPLAVSVPVKAIWADPKEVHDAG
                     GISVGDRWKALANALNIPLDQLSARINANPKGRFIYLARQVNPDMADYIKKLKLPGIH
                     LREESRRYYPSGEVTAHLIGFTNVDSQGIEGVEKSFDKWLTGQPGERIVRKDRYGRVI
                     EDISSTDSQAAHNLALSIDERLQALVYRELNNAVAFNKAESGSAVLVDVNTGEVLAMA
                     NSPSYNPNNLSGTPKEAMRNRTITDVFEPGSTVKPMVVMTALQRGVVRENSVLNTIPY
                     RINGHEIKDVARYSELTLTGVLQKSSNVGVSKLALAMPSSALVDTYSRFGLGKATNLG
                     LVGERSGLYPQKQRWSDIERATFSFGYGLMVTPLQLARVYATIGSYGIYRPLSITKVD
                     PPVPGERVFPESIVRTVVHMMESVALPGGGGVKAAIKGYRIAIKTGTAKKVGPDGRYI
                     NKYIAYTAGVAPASQPRFALVVVINDPQAGKYYGGAVSAPVFGAIMGGVLRTMNIEPD
                     ALTTGDKNEFVINQGEGTGGRS"
     gene            9004..10491
                     /gene="murE"
                     /locus_tag="ACHOSC_RS08160"
                     /old_locus_tag="pgaptmp_001632"
     CDS             9004..10491
                     /gene="murE"
                     /locus_tag="ACHOSC_RS08160"
                     /old_locus_tag="pgaptmp_001632"
                     /EC_number="6.3.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016881 - acid-amino acid ligase activity
                     [Evidence IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanyl-D-glutamate--2,
                     6-diaminopimelate ligase"
                     /protein_id="HGU4547594.1"
                     /translation="MADRNLRDLLAPWVPDAPSRALREMTLDSRVAAAGDLFVAVVGH
                     QADGRRYIPQAIAQGVTAIIAEAKGEATDGEIREMHGVPVIYLSQLNERLSALAGRFY
                     HEPSDNLRLVGVTGTNGKTTTTQLLAQWSQLLGETSAVMGTVGNGLLGKVIPTENTTG
                     SAVDVQHELAGLVDQGATFCAMEVSSHGLVQHRVAALKFAASVFTNLSRDHLDYHGDM
                     EHYEAAKWLLYSEHHCGQAIVNADDEVGRRWLAKLPDAVAVSMEDHINPNCHGRWLKA
                     TEVNYHDSGATIRFSSSWGDGEIESHLMGAFNVSNLLLALATLLALGYPLADLLKTAA
                     RLQPVCGRMEVFTAPGKPTVVVDYAHTPDALEKALQAARLHCAGKLWCVFGCGGDRDK
                     GKRPLMGAIAEEFADVAVVTDDNPRTEEPRAIINDILAGMLDAGHAKVMEGRAEAVTC
                     AVMQAKENDVVLVAGKGHEDYQIVGNQRLDYSDRVTVARLLGVIA"
     gene            10488..11846
                     /gene="murF"
                     /locus_tag="ACHOSC_RS08165"
                     /old_locus_tag="pgaptmp_001633"
     CDS             10488..11846
                     /gene="murF"
                     /locus_tag="ACHOSC_RS08165"
                     /old_locus_tag="pgaptmp_001633"
                     /EC_number="6.3.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0047480 -
                     UDP-N-acetylmuramoyl-tripeptide-D-alanyl-D-alanine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-tripeptide--D-alanyl-D-
                     alanine ligase"
                     /protein_id="HGU4547595.1"
                     /translation="MISVTLSQLTDILNGELQGADITLDAVTTDTRKLTPGCLFVALK
                     GERFDAHDFADQAKAGGAGALLVSRPLDIDLPQLIVKDTRLAFGELAAWVRQQVPARV
                     VALTGSSGKTSVKEMTAAILSQCGNTLYTAGNLNNDIGVPMTLLRLTPEYDYAVIELG
                     ANHQGEIAWTVSLTRPEAALVNNLAAAHLEGFGSLAGVAKAKGEIFSGLPENGIAIMN
                     ADNNDWLNWQSVIGSRKVWRFSPNAANSDFTATNIHVTSHGTEFTLQTPTGSVDVLLP
                     LPGRHNIANALAAAALSMSVGATLDAIKAGLANLKAVPGRLFPIQLAENQLLLDDSYN
                     ANVGSMTAAVQVLAEMPGYRVLVVGDMAELGAESEACHVQVGEAAKAAGIDRVLSVGK
                     QSHAISTASGVGEHFADKTALITRLKSLIAEQQVITILVKGSRSAAMEEVVRALQENG
                     TC"
     gene            11840..12922
                     /gene="mraY"
                     /locus_tag="ACHOSC_RS08170"
                     /old_locus_tag="pgaptmp_001634"
     CDS             11840..12922
                     /gene="mraY"
                     /locus_tag="ACHOSC_RS08170"
                     /old_locus_tag="pgaptmp_001634"
                     /EC_number="2.7.8.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008963 -
                     phospho-N-acetylmuramoyl-pentapeptide-transferase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-N-acetylmuramoyl-pentapeptide-
                     transferase"
                     /protein_id="HGU4547596.1"
                     /translation="MLVWLAEHLVKYYSGFNVFSYLTFRAIVSLLTALFISLWMGPRM
                     IAHLQKLSFGQVVRNDGPESHFSKRGTPTMGGIMILTAIVISVLLWAYPSNPYVWCVL
                     VVLVGYGVIGFVDDYRKVVRKDTKGLIARWKYFWMSVIALGIAFALYLAGKDTPATQL
                     VVPFFKDVMPQLGLFYILLAYFVIVGTGNAVNLTDGLDGLAIMPTVFVAGGFALVAWA
                     TGNMNFASYLHIPYLRHAGELVIVCTAIVGAGLGFLWFNTYPAQVFMGDVGSLALGGA
                     LGIIAVLLRQEFLLVIMGGVFVVETLSVILQVGSFKLRGQRIFRMAPIHHHYELKGWP
                     EPRVIVRFWIISLMLVLIGLATLKVR"
     gene            12925..14241
                     /gene="murD"
                     /locus_tag="ACHOSC_RS08175"
                     /old_locus_tag="pgaptmp_001635"
     CDS             12925..14241
                     /gene="murD"
                     /locus_tag="ACHOSC_RS08175"
                     /old_locus_tag="pgaptmp_001635"
                     /EC_number="6.3.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008764 -
                     UDP-N-acetylmuramoylalanine-D-glutamate ligase activity
                     [Evidence IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanine--D-glutamate
                     ligase"
                     /protein_id="HGU4547597.1"
                     /translation="MADYQGKNVVIIGLGLTGLSCVDFFLARGVTPRVMDTRMTPPGL
                     DKLPEAVERHTGSLNDEWLMAADLIVASPGIALAHPSLSAAADAGIEIVGDIELFCRE
                     AQAPIVAITGSNGKSTVTTLVGEMAKAAGVNVGVGGNIGLPALMLLDAECELYVLELS
                     SFQLETTSSLQAVAATILNVTEDHMDRYPFGLQQYRAAKLRIYENAKVCVVNADDALT
                     MPIRGADERCVSFGVNMGDYHLNHQQGETWLRVKGEKVLNVKEMKLSGQHNYTNALAA
                     LALADAAGLPRASSLKALTTFTGLPHRFEVVLEHNGVRWINDSKATNVGSTEAALNGL
                     QVDGTLHLLLGGDGKSADFSPLARYLNGDNVRLYCFGRDGAQLAALRPEVAEQTETME
                     QAMRLLAPRVQPGDMVLLSPACASLDQFKNFEQRGNEFARLAKELG"
     gene            14241..15485
                     /gene="ftsW"
                     /locus_tag="ACHOSC_RS08180"
                     /old_locus_tag="pgaptmp_001636"
     CDS             14241..15485
                     /gene="ftsW"
                     /locus_tag="ACHOSC_RS08180"
                     /old_locus_tag="pgaptmp_001636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsW"
                     /protein_id="HGU4547598.1"
                     /translation="MRLSLPRLKMPRLPGFSILVWISTALKGWVMGSREKDTDSLIMY
                     DRTLLWLTFGLAAIGFIMVTSASMPIGQRLTNDPFFFAKRDGVYLILAFILAIITLRL
                     PMEFWQRYSATMLLGSIILLMIVLVVGSSVKGASRWIDLGLLRIQPAELTKLSLFCYI
                     ANYLVRKGDEVRNNLRGFLKPMGVILVLAVLLLAQPDLGTVVVLFVTTLAMLFLAGAK
                     LWQFIAIIGMGISAVVLLILAEPYRIRRVTAFWNPWEDPFGSGYQLTQSLMAFGRGEL
                     WGQGLGNSVQKLEYLPEAHTDFIFAIIGEELGYVGVVLALLMVFFVAFRAMSIGRKAL
                     EIDHRFSGFLACSIGIWFSFQALVNVGAAAGMLPTKGLTLPLISYGGSSLLIMSTAIM
                     MLLRIDYETRLEKAQAFVRGSR"
     gene            15482..16549
                     /gene="murG"
                     /locus_tag="ACHOSC_RS08185"
                     /old_locus_tag="pgaptmp_001637"
     CDS             15482..16549
                     /gene="murG"
                     /locus_tag="ACHOSC_RS08185"
                     /old_locus_tag="pgaptmp_001637"
                     /EC_number="2.4.1.227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050511 -
                     undecaprenyldiphospho-muramoylpentapeptide
                     beta-N-acetylglucosaminyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyldiphospho-muramoylpentapeptide
                     beta-N-acetylglucosaminyltransferase"
                     /protein_id="HGU4547599.1"
                     /translation="MSGQGKRLMVMAGGTGGHVFPGLAVAHHLMAQGWQVRWLGTADR
                     MEADLVPKHGIEIDFIRISGLRGKGIKALIAAPLRIFNAWRQARAIMKAYKPDVVLGM
                     GGYVSGPGGLAAWSLGIPVVLHEQNGIAGLTNKWLAKIATKVMQAFPGAFPNAEVVGN
                     PVRTDVLALPLPQQRLAGREGPVRVLVVGGSQGARILNQTMPQVAAKLGDSVTIWHQS
                     GKGSQQSVEQAYAEAGQPQHKVTEFIDDMAAAYAWADVVVCRSGALTVSEIAAAGLPA
                     LFVPFQHKDRQQYWNALPLEKAGAAKIIEQPQLSVDAVANTLAGWSRETLLTMAERAR
                     AASIPDATERVANEVSRAARA"
     gene            16603..18078
                     /gene="murC"
                     /locus_tag="ACHOSC_RS08190"
                     /old_locus_tag="pgaptmp_001638"
     CDS             16603..18078
                     /gene="murC"
                     /locus_tag="ACHOSC_RS08190"
                     /old_locus_tag="pgaptmp_001638"
                     /EC_number="6.3.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008763 - UDP-N-acetylmuramate-L-alanine
                     ligase activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate--L-alanine ligase"
                     /protein_id="HGU4547600.1"
                     /translation="MNTQQLAKLRSIVPEMRRVRHIHFVGIGGAGMGGIAEVLANEGY
                     QISGSDLAPNPVTQQLMNLGATIYFNHRPENVRDASVVVVSSAISAYNPEIVAAHEAR
                     IPVIRRAEMLAELMRFRHGIAIAGTHGKTTTTAMVSSIYAEAGLDPTFVNGGLVKAAG
                     VHARLGHGRYLIAEADESDASFLHLQPMVAIVTNIEADHMDTYQGDFENLKQTFINFL
                     HNLPFYGRAVMCVDDPVIRELLPRVGRQTTTYGFSEDADVRVEDYQQIGPQGHFTLLR
                     QDKEPMRVTLNAPGRHNALNAAAAVAVATEEGIDDEAILRALESFQGTGRRFDFLGEF
                     PLEPVNGKSGTAMLVDDYGHHPTEVDATIKAARAGWPDKNLVMLFQPHRFTRTRDLYD
                     DFANVLTQVDTLLMLEVYPAGEAPIPGADSRSLCRTIRGRGKIDPILVPDPAQVAEML
                     APVLTGNDLILVQGAGNIGKIARSLAEIKLKPQTPEEEQHD"
     gene            18071..18991
                     /gene="ddlB"
                     /locus_tag="ACHOSC_RS08195"
                     /old_locus_tag="pgaptmp_001639"
     CDS             18071..18991
                     /gene="ddlB"
                     /locus_tag="ACHOSC_RS08195"
                     /old_locus_tag="pgaptmp_001639"
                     /EC_number="6.3.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--D-alanine ligase"
                     /protein_id="HGU4547601.1"
                     /translation="MTDKIAVLLGGTSAEREVSLNSGAAVLAGLREGGIDAYPVDPKE
                     VDVTQLKSMGFQKVFIALHGRGGEDGTLQGMLELMGLPYTGSGVMASALSMDKLRSKL
                     LWQGAGLPVAPWVALTRAEFEKGLSDKQLAEISALGLPVIVKPSREGSSVGMSKVVAE
                     NALQDALRLAFQHDEEVLIEKWLSGPEFTVAILGEEILPSIRIQPSGTFYDYEAKYLS
                     DETQYFCPAGLEASQEANLQALVLKAWTTLGCKGWGRIDVMLDSDGQFYLLEANTSPG
                     MTSHSLVPMAARQAGMSFSQLVVRILELAD"
     gene            18993..19823
                     /gene="ftsQ"
                     /locus_tag="ACHOSC_RS08200"
                     /old_locus_tag="pgaptmp_001640"
     CDS             18993..19823
                     /gene="ftsQ"
                     /locus_tag="ACHOSC_RS08200"
                     /old_locus_tag="pgaptmp_001640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0090529 - cell septum assembly [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsQ"
                     /protein_id="HGU4547602.1"
                     /translation="MSQAALNTRNSEEEVSSRRNNGTRLAGILFLLTVLTTVLVSGWV
                     VLGWMEDAQRLPLSKLVLTGERHYTRNDDIRQSILALGEPGTFMTQDVNIIQTQIEQR
                     LPWIKQVSVRKQWPDELKIHLVEYVPIARWNDQHMVDAEGNTFSVPPDRTSKQVLPML
                     YGPEGSANEVLQGYREMGQMLAKDRFTLKEAAMTARRSWQLTLNNDIKLNLGRGDTMK
                     RLARFVELYPVLQQQAQTDGKRISYVDLRYDSGAAVGWAPLPPEESTQQQNHAQAEQQ
                     "
     gene            19820..21082
                     /gene="ftsA"
                     /locus_tag="ACHOSC_RS08205"
                     /old_locus_tag="pgaptmp_001641"
     CDS             19820..21082
                     /gene="ftsA"
                     /locus_tag="ACHOSC_RS08205"
                     /old_locus_tag="pgaptmp_001641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsA"
                     /protein_id="HGU4547603.1"
                     /translation="MIKATDRKLVVGLEIGTAKVAALVGEVLPDGMVNIIGVGSCPSR
                     GMDKGGVNDLESVVKCVQRAIDQAELMADCQISSVYLALSGKHISCQNEIGMVPISEE
                     EVTQEDVENVVHTAKSVRVRDEHRVLHVIPQEYAIDYQEGIKNPVGLSGVRMQAKVHL
                     ITCHNDMAKNIVKAVERCGLKVDQLIFAGLASSYSVLTEDERELGVCVVDIGGGTMDI
                     AVYTGGALRHTKVIPYAGNVVTSDIAYAFGTPPSDAEAIKVRHGCALGSIVGKDESVE
                     VPSVGGRPPRSLQRQTLAEVIEPRYTELLNLVNEEILQLQEKLRQQGVKHHLAAGIVL
                     TGGAAQIEGLAACAQRVFHTQVRIGAPLNITGLTDYAQEPYYSTAVGLLHYGKESHLN
                     GEAEVEKRVTASVGSWIKRLNSWLRKEF"
     gene            21143..22294
                     /gene="ftsZ"
                     /locus_tag="ACHOSC_RS08210"
                     /old_locus_tag="pgaptmp_001642"
     CDS             21143..22294
                     /gene="ftsZ"
                     /locus_tag="ACHOSC_RS08210"
                     /old_locus_tag="pgaptmp_001642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015965916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsZ"
                     /protein_id="HGU4547604.1"
                     /translation="MFEPMELTNDAVIKVIGVGGGGGNAVEHMVRERIEGVEFFAVNT
                     DAQALRKTAVGQTIQIGSGITKGLGAGANPEVGRNAADEDRDALRAALEGADMVFIAA
                     GMGGGTGTGAAPVVAEVAKDLGILTVAVVTKPFNFEGKKRMAFAEQGITELSKHVDSL
                     ITIPNDKLLKVLGRGISLLDAFGAANDVLKGAVQGIAELITRPGLMNVDFADVRTVMS
                     EMGYAMMGSGVASGEDRAEEAAEMAISSPLLEDIDLSGARGVLVNITAGFDLRLDEFE
                     TVGNTIRAFASDNATVVIGTSLDPDMNDELRVTVVATGIGMDKRPEITLVTNKQVQQP
                     VMDRYQQHGMAPLTQEQKPVAKVVNDNAPQTAKEPDYLDIPAFLRKQAD"
     gene            22395..23312
                     /gene="lpxC"
                     /locus_tag="ACHOSC_RS08215"
                     /old_locus_tag="pgaptmp_001643"
     CDS             22395..23312
                     /gene="lpxC"
                     /locus_tag="ACHOSC_RS08215"
                     /old_locus_tag="pgaptmp_001643"
                     /EC_number="3.5.1.108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008759 - UDP-3-O-[3-hydroxymyristoyl]
                     N-acetylglucosamine deacetylase activity [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-3-O-acyl-N-acetylglucosamine deacetylase"
                     /protein_id="HGU4547605.1"
                     /translation="MIKQRTLKRIVQATGVGLHTGKKVTLTLRPAPANTGVIYRRTDL
                     NPPVDFPADAKSVRDTMLCTCLVNEHDVRISTVEHLNAALAGLGIDNIVIEVNAPEIP
                     IMDGSAAPFVYLLLDAGIDELNCAKKFVRIKETVRVEDGDKWAEFKPYNGFSLDFTID
                     FNHPAIDSSNQRYAMNFSADAFMRQISRARTFGFMRDIEYLQSRGLCLGGSFDCAIVV
                     DDYRVLNEDGLRFEDEFVRHKMLDAIGDLFMCGHNIIGAFTAYKSGHALNNKLLQAVL
                     AKQEAWEYVTFQDDAELPLAFKAPSAVLA"
BASE COUNT         5406 a         5583 c         6722 g         5651 t
ORIGIN      
        1 aacattatgg attattatgc tgtggtaaat gactcattcc acggcaatgg attctgtttt
       61 tatcagaacc cgtatcttta tgttttccga attttactca ttttgctttt tcttatttta
      121 tatgcatgat aaatcatatt cttcaggatt atttctctgc attccaataa gggaaaggga
      181 gttaagtgtg acagtggagt taagtatgcc agaggtacaa acagatcatt cagagacggc
      241 ggagttaagc aagccacagc tacgcatggt cgatctcaac ttattaaccg ttttcgatgc
      301 cgtgatgcag gagcaaaaca ttacccgtgc cgctcatgtt ctgggtatgt cgcaacctgc
      361 ggtcagtaac gctgttgcac gcctgaaggt gatgtttaat gacgagcttt ttgttcgtta
      421 tggccgtggt attcaaccga ctgctcgcgc atttcaactt tttggctcag ttcgtcaggc
      481 attgcaacta gtacaaaatg aattgcctgg ttcaggtttt gaacccgcga gcagtgaacg
      541 tgtatttcat ctttgtgttt gcagcccgtt agacagcatt ctgacctcgc agatttataa
      601 tcacattgag cagattgcgc caaatataca tgttatgttc aagtcttcat taaatcagaa
      661 cactgaacat cagctgcgtt atcaggaaac ggagtttgtg attagttatg aagacttcca
      721 tcgtcctgaa tttaccagcg tgccattatt taaagatgaa atggtgctgg tagccagcaa
      781 aaatcatcca acaattaagg gcccgttact gaaacatgat gtttataacg aacaacatgc
      841 ggcggtttcg ctcgatcgtt tcgcgtcatt tagtcaacct tggtatgaca cggtagataa
      901 gcaagccagt atcgcgtatc agggcatggc aatgatgagc gtacttagcg tggtgtcgca
      961 aacgcatttg gtcgctattg cgccgcgttg gctggctgaa gagttcgctg aatccttaga
     1021 attacaggta ttaccgctgc cgttaaaaca aaatagcaga acctgttatc tctcctggca
     1081 tgaagctgcc gggcgcgata aaggccatca gtggatggaa gagcaattag tctcaatttg
     1141 caaacgctaa ctgaatgcag aataggtcag gcatgaatgt ctggtttatt ctgtattttt
     1201 tattgaatgt agaattttat tctgaatgtg tgggctctct gttttaggat taattaaaaa
     1261 aatagagaaa ttgctgtaag ttgtgggatt cagccgattt attatcaatt taatccttcg
     1321 taatggagga ttttatcgtt tcttttcacc tttcctcctg tttattctta ttaccctgcg
     1381 tttatgtctc tggctgccaa ttgcttaagc aagatcggac ggttaatgtg ttttacacat
     1441 tttttccgtc aaacagtgag gcaggccatg gagatgttgt ctggagccga gatggtcgtc
     1501 cgatcgctta tcgatcaggg cgttaaacaa gtattcggtt atcccggagg cgcagtcctt
     1561 gatatttacg atgcattgca taccgtgggt ggtatcgatc atgttttagt tcgtcatgag
     1621 caggcggcag tgcatatggc cgatggtctg gcgcgcgcga ccggggaagt cggcgtcgtg
     1681 ctggtaacgt cgggtccagg ggcgaccaat gcgattaccg gcatcgccac cgcttatatg
     1741 gattccattc cattagttgt cctttccggg caggtagcga cctcgttgat aggttacgat
     1801 gcctttcagg agtgcgacat ggtggggatt tcgcgaccgg tggttaaaca cagttttctg
     1861 gttaagcaaa cggaagacat tccgcaggtg ctgaaaaagg ctttctggct ggcggcaagt
     1921 ggtcgcccag gaccagtggt cgttgattta ccgaaagata ttcttaatcc ggcgaacaaa
     1981 ttaccctatg tctggcctga gtcggtcagt atgcgttctt acaatcccac tactaccgga
     2041 cataaagggc aaattaagcg cgctctgcaa acgctggtag cggcaaaaaa accggttgtc
     2101 tacgtaggcg gtggggcaat catggcgggc tgccatcagc agttgaaaga aacggtggag
     2161 gcgttgaatc tgcccgttgt ttcctcattg atggggctgg gggcgtttcc ggcaacgcat
     2221 cgtcaggcac tgggcatgct gggaatgcac ggtacctacg aagccaatat gacgatgcat
     2281 aacgcggatg tgattttcgc cgtcggggta cgatttgatg accgaacgac gaacaatctg
     2341 gcaaagtact gcccaaatgc cactgttctg catatcgata ttgatcctac ttccatttct
     2401 aaaaccgtga ctgcggatat cccgattgtg ggggatgctc gccaggtcct cgaacaaatg
     2461 cttgaactct tgtcgcaaga atccgcccat caaccactgg atgagatccg cgactggtgg
     2521 cagcaaattg aacagtggcg cgctcgtcag tgcctgaaat atgacactca cagtgaaaag
     2581 attaaaccgc aggcggtgat cgagactctt tggcggttga cgaagggaga cgcttacgtg
     2641 acgtccgatg tcgggcagca ccagatgttt gctgcacttt attatccatt cgacaaaccg
     2701 cgtcgctgga tcaattccgg tggcctcggc acgatgggtt ttggtttacc tgcggcactg
     2761 ggcgtcaaaa tggcgttgcc agaagaaacc gtggtttgcg tcactggcga cggcagtatt
     2821 cagatgaaca tccaggaact gtctaccgcg ttgcaatacg agttgccggt actggtggtg
     2881 aatctcaata accgctatct ggggatggtg aaacagtggc aggacatgat ctattccggc
     2941 cgtcattcac aatcttatat gcaatcacta cccgatttcg tccgtctggc ggaagcctat
     3001 ggtcacgtcg ggatccagat ctctcatccg catgagctgg aaagcaaact tagcgaggcg
     3061 ctggaacagg tgtgcaataa tcgcctggtg tttgttgatg ttaccgtcga tggcagtgag
     3121 cacgtctacc cgatgcagat tcgcgggggc ggaatggatg aaatgtggtt aagcaaaacg
     3181 gagagaacct gattatgcgc cggatattat cagtcttact cgaaaatgaa tcaggcgcgt
     3241 tatcccgcgt gattggcctt ttttcccagc gtggctacaa cattgaaagc ctgaccgttg
     3301 cgccaaccga cgatccgaca ttatcgcgta tgaccatcca gaccgtgggc gatgaaaaag
     3361 tgcttgagca gatcgaaaag caattacaca agctggtcga tgtcttgcgc gtgagtgagt
     3421 tggggcaggg cgcgcatgtt gagcgggaaa tcatgctggt gaaaattcag gccagcggtt
     3481 acgggcgtga cgaagtgaaa cgtaatacgg aaatattccg tgggcaaatt atcgatgtca
     3541 ccccctcgct ttataccgtt caattagcag gcaccagcga taagcttgat gcatttttag
     3601 catcgattcg cgatgtggcg aaaattgtgg aagttgctcg ctctggtgtg gtcggacttt
     3661 cgcgcggcga taaaataatg cgttgagaat gatctcagtg cgcaatttgc agcccaacat
     3721 gtcacgttgg gctttttttg cgaaatcagt gggaacctgg aataaaagca gttgccgcag
     3781 ttaattttct gcgcttagat gttaatgaat ttaacccata ccagtacaat ggctatggtt
     3841 tttacatttt acgcaagggg caattgtgaa actggatgaa atcgctcggc tggcgggagt
     3901 gtcgcggacc actgcaagct atgttattaa cggcaaagcg aagcaatacc gtgtgagcga
     3961 caaaaccgtt gaaaaagtca tggctgtggt gcgtgagcac aattaccacc cgaacgccgt
     4021 ggcagctggg cttcgtgctg gacgcacacg ttctattggt cttgtgatcc ccgatctgga
     4081 gaacaccagc tatacccgca tcgctaacta tcttgagcgc caggcgcggc aacggggtta
     4141 tcaactgctg attgcctgct cagaagatca gccagacaac gaaatgcgct gcattgagca
     4201 ccttttacag cgtcaggttg atgccattat tgtttcgacg tcgttgccac ctgagcatcc
     4261 tttttatcaa cgctgggcta acgacccgtt cccgattgtc gcgctggacc gcgccctcga
     4321 tcgtgaacat ttcaccagcg tggttggtgc cgatcaggat gatgccgaaa tgctggcgga
     4381 agagttacgt aagtttcccg ccgagacggt gctttatctt ggcgcgctgc cggagctttc
     4441 tgtcagcttc ctgcgtgaac aaggtttccg tactgcctgg aaagatgatc cgcgcgaagt
     4501 gcatttcctg tatgccaaca gctatgagcg ggaggcggct gcccagttat tcgaaaaatg
     4561 gctggaaacg catccgatgc cgcaggcgct gttcacaacg tcgtttgcgt tgttgcaagg
     4621 ggtgatggat gtcacgctac gtcgcgatgg taaactgcct tctgacctgg caattgccac
     4681 ctttggcgat aacgaactgc tcgacttctt acagtgcccg gtgcttgcag tggctcaacg
     4741 tcaccgcgat gtcgcagagc gtgtgctgga gattgtcctg gcaagcctgg acgaaccgcg
     4801 taagccaaaa cctggtttaa cgcgcattaa acgtaatctc tatcgccgcg gtgtgctcag
     4861 ccgtagctaa gccgcgaaca aaaatacgcg ccaggtgaat ttccctctgg cgcgtagagt
     4921 acgggaatgg acatcaatat ccttaaagta aataagacta ttcctgacta ttattgataa
     4981 gttcttttaa acccgcccgt taattaactc accagctgaa attcacaata attaagtgat
     5041 atcgacagcg cgtttttgca ttattttgtt acatgcagag atgaattgcc gatttaacaa
     5101 gcacttttct ttgcttttgc gcaaacccgc tggcatcaag cgccacacag acgcaacagg
     5161 gactgttaac cggggaagat atgtcctaaa atgccgctcg cgtcgcaaac tgacacttta
     5221 tatttgctgt ggaaaatagt gagtcatttt aaaacggtga tgacgatgag ggattttttc
     5281 ttacagctat tcataacgtt aatttgctgc gcacgttgga cgtaaaataa acaacgctga
     5341 tattagccgt aaacatcggg ttttttacct cggtatgcct tgtgactggc ttgacaagct
     5401 tttcctccgc tccgtaaact cctttcagtg ggaaattgtg gggcaaagtg ggaataaggg
     5461 gtgaggctgg catgttccgg ggagcaacgt tagtcaatct cgacagcaaa gggcgcttat
     5521 cagtgcctac ccgttaccgg gaacagctgc ttgagaacgc tgccggtcaa atggtttgca
     5581 ccattgacat tcatcacccg tgcctgctgc tttaccccct acctgaatgg gaaattatcg
     5641 agcaaaaatt atcgcgtctg tcgagcatga acccggttga gcgccgtgtg cagcgcctac
     5701 tgttaggtca tgccagcgaa tgtcagatgg atggcgcagg tcgattgtta atcgcgccag
     5761 tactgcggca acatgccggg ctgacaaaag aagtgatgct ggttggacag ttcaacaagt
     5821 ttgagctgtg ggatgaaaca acctggcatc aacaggtcaa ggaagatatc gacgcagagc
     5881 agttggctac cggagactta tcggagcgac tgcaggactt gtctctataa aatgatggaa
     5941 aactataaac atactacggt gctgctggat gaagccgtta atggcctcaa tatccgtcct
     6001 gatggcatct acattgatgg gacttttggt cgcggtggtc actcacgtct gatcctctcg
     6061 cagcttggcg aagaggggcg tttgctggcg atcgatcgcg acccgcaggc tatcgccgtt
     6121 gcgaagacta ttgatgatcc gcgcttctcc atcatccacg gacctttctc cgcgctgggc
     6181 gaatacgttg ccgagcgcga tcttatcggc aagatcgacg gcattctcct cgatcttggc
     6241 gtctcttcac cgcaacttga tgatgctgag cgcggctttt cctttatgcg cgatggtccg
     6301 ctggacatgc gtatggaccc aacccgtggg cagtcagccg ctgaatggct acaaaccgca
     6361 gaagaagccg atatcgcctg ggtattgaaa acctatggtg aagagcgttt tgccaaacgc
     6421 attgcccgcg ccattgtcga gcgtaaccgc gaacagccga tgacccgcac caaagaactg
     6481 gcggaagtcg tggctgctgc aacgccggtg aaagataagt ttaaacatcc cgcgacccgt
     6541 accttccagg cggtgcgcat ttgggtaaac agtgaactgg aggagataga gcaggcgcta
     6601 aaaagctcgc tcaacgtgct ggccccgggt gggcggcttt cgatcatcag cttccactcg
     6661 ctggaagacc gtattgtgaa acgttttatg cgtgaaaaca gccgcggtcc gcaagttccg
     6721 gcagggttac cgatgactga agagcagctc aaaaaactgg gtggccgtca gctgcgagca
     6781 ctaggcaagt taatgccggg cgaagaagag gtggctgaga accctcgtgc ccgtagttca
     6841 gttctgcgta ttgcagagag gacgaatgca tgatcagcag agtgacagaa gctctaagca
     6901 aagttaaagg atcgatggga agccacgagc gccatgcatt gcctggtgtt atcggtgacg
     6961 atcttttgcg atttgggaag ctgccactct gcctgttcat ttgcattatt ttgacggcgg
     7021 tgactgtggt aaccacggcg caccataccc gtttactgac cgctcagcgc gaacaactgg
     7081 tgctggagcg agatgcttta gacattgaat ggcgcaacct gatccttgaa gagaatgcgc
     7141 tcggcgacca tagccgggtg gaaaggatcg ccacggaaaa gctgcaaatg cagcatgttg
     7201 atccgtcaca agaaaatatc gtagtgcaaa aataaggata aacgcgacgc atgaaagcag
     7261 cggcgaaaac gcagaaacca aaacgtcagg aagaacatgc caactttatc agttggcgtt
     7321 ttgcgttgtt atgcggctgt attctcctgg cgctggcttt tctgctcgga cgcgtagcgt
     7381 ggttacaagt tatctccccg gatatgctgg tgaaagaggg cgacatgcgt tctcttcgcg
     7441 ttcagcaagt ttccacctcc cgcggcatga ttactgaccg ttctggtcgc ccgttagcgg
     7501 tgagcgtgcc ggtaaaagcg atttgggctg acccgaaaga agtgcatgac gctggcggta
     7561 tcagcgtcgg tgaccgctgg aaggcgctgg ctaacgcgct caatattccg ctggatcagc
     7621 tttcagcccg cattaacgcc aacccgaaag ggcgctttat ttatctggcg cgtcaggtga
     7681 accctgacat ggcggactac atcaaaaaac tgaaactgcc ggggattcat ctgcgtgaag
     7741 agtctcgccg ttactatccg tccggcgaag tgactgctca cctcatcggc tttactaacg
     7801 tcgatagtca agggattgag ggcgttgaga agagtttcga taaatggctt accgggcagc
     7861 cgggtgagcg cattgtgcgt aaagaccgct atggtcgcgt aattgaagat atttcttcta
     7921 ctgacagcca ggcagcgcac aacctggcgc tgagtattga tgaacgcctg caggcgctgg
     7981 tttatcgcga actgaacaac gcggtggcct ttaacaaggc tgaatctggt agcgccgtgc
     8041 tggtggatgt caacaccggt gaagtgctgg cgatggctaa cagcccgtca tacaacccta
     8101 acaatctgag cggcacgccg aaagaggcga tgcgtaaccg taccatcacc gacgtgtttg
     8161 aaccgggctc aacggttaaa ccgatggtgg taatgaccgc gttgcaacgt ggcgtggtgc
     8221 gggaaaactc ggtactcaat accattcctt atcgaattaa cggccacgaa atcaaagacg
     8281 tggcacgcta cagcgaatta accctgaccg gggtattaca gaagtcgagt aacgtcggtg
     8341 tttccaagct ggcgttagcg atgccgtcct cagcgttagt agatacttac tcacgttttg
     8401 gactgggaaa agcgaccaat ttggggttgg tcggagaacg cagtggctta tatcctcaaa
     8461 aacaacggtg gtctgacata gagagggcca ccttctcttt cggctacggg ctaatggtaa
     8521 caccgttaca gttagcgcga gtctacgcaa ctatcggcag ctacggcatt tatcgcccac
     8581 tgtcgattac caaagttgac cccccggttc ccggtgaacg tgtcttcccg gaatccattg
     8641 tccgcactgt ggtgcatatg atggaaagcg tggcgctacc aggcggcggc ggcgtgaagg
     8701 cggcgattaa aggctatcgt atcgccatta aaaccggtac cgcgaaaaag gtcgggccgg
     8761 acggtcgcta catcaataaa tatattgctt ataccgcagg cgttgcgcct gcgagtcagc
     8821 cgcgcttcgc gctggttgtt gttatcaacg atccgcaggc gggtaaatac tacggcggcg
     8881 ccgtttcagc gccggtcttt ggtgccatca tgggcggcgt attgcgtacc atgaacatcg
     8941 agccggatgc gctgacaacg ggcgataaaa atgaatttgt gattaatcaa ggcgagggga
     9001 caggtggcag atcgtaattt gcgcgacctt cttgctccgt gggtgccaga cgcaccttcg
     9061 cgagcactgc gagagatgac actcgacagc cgtgtggctg cggcgggcga tctctttgta
     9121 gctgtagtag gtcatcaggc ggacgggcgt cgatatatcc cgcaggcgat agcgcaaggt
     9181 gtgactgcca ttattgcaga ggcgaaaggt gaggcgaccg acggtgaaat ccgtgaaatg
     9241 cacggcgtac cggtcatcta tctcagccag ctcaacgagc gtttatctgc actggcgggc
     9301 cgcttttacc atgaaccctc tgacaattta cgtctcgtag gcgtaacggg caccaacggc
     9361 aaaaccacga ctacccagct gctggcgcag tggagccaac tgcttggcga aaccagcgcg
     9421 gtaatgggca ccgttggtaa cggtctgctg gggaaagtga tcccgacaga aaataccacc
     9481 ggttcggcag tcgatgttca gcatgagctg gcggggctgg tggatcaggg cgcgacgttt
     9541 tgcgcaatgg aagtttcctc ccacgggctg gtacagcacc gtgtggcggc attgaaattt
     9601 gcggcgtcgg tctttaccaa cttaagccgc gatcaccttg attatcatgg tgatatggaa
     9661 cactacgaag ccgcgaaatg gctgctttat tctgaacatc attgtggtca ggcgattgtt
     9721 aacgccgacg atgaagtggg ccgccgctgg ctggcaaaac tgccggacgc ggttgcggta
     9781 tcaatggaag atcatatcaa tccgaactgt cacggacgct ggttgaaagc gaccgaagtg
     9841 aactatcacg acagcggcgc gaccattcgc tttagttcaa gttggggcga tggcgaaatt
     9901 gaaagccatc tgatgggcgc ttttaacgtc agcaacctgc tgctcgcgct ggcgacactg
     9961 ctggcacttg gctacccgct ggctgatctg ttaaaaaccg ccgcgcgtct gcaaccggtt
    10021 tgcggacgta tggaagtgtt cactgcgcca ggcaaaccga cggtggtggt ggattacgcg
    10081 catacgccgg atgcactgga aaaagcctta caggcggcgc gcctgcactg tgcgggcaag
    10141 ctgtggtgcg tctttggctg tggtggcgat cgcgataaag gtaagcgtcc actgatgggc
    10201 gcaattgccg aagagtttgc tgacgtggcg gtggtgacgg acgataaccc gcgtaccgaa
    10261 gaaccgcgtg ccatcatcaa cgatattctg gcgggaatgt tagatgccgg acatgccaaa
    10321 gtgatggaag gccgtgctga agcggtgact tgcgccgtta tgcaggctaa agagaatgat
    10381 gtggtactgg tcgcgggcaa aggccatgag gattaccaga ttgttggcaa tcagcgtctg
    10441 gactactccg atcgcgtcac ggtggcgcgt ctgctggggg tgattgcatg attagcgtaa
    10501 cccttagcca acttaccgat attctcaacg gtgaactgca aggtgcagat atcacccttg
    10561 atgctgtaac cactgatacc cgaaaactga cgccgggctg cctgtttgtt gccctgaaag
    10621 gcgaacgttt tgatgcccac gattttgccg accaggcgaa agctggcggc gcaggcgcac
    10681 tactggttag ccgtccgctg gacatcgacc tgccgcagtt aatcgtcaag gatacgcgtc
    10741 tggcgtttgg tgaactggct gcatgggttc gccagcaagt tccggcgcgc gtggttgctc
    10801 tgacggggtc ctccggcaaa acctccgtta aagagatgac ggcggcgatt ttaagccagt
    10861 gcggcaacac gctttatacg gcaggcaatc tcaacaacga catcggtgta ccgatgacgc
    10921 tgttgcgctt aacgccggaa tacgattacg cagttattga acttggcgcg aaccatcagg
    10981 gcgaaatagc ctggactgtg agtctgactc gcccggaagc tgcgctggtc aacaacctgg
    11041 cagcggcgca tctggaaggt tttggctcgc ttgcgggtgt cgcgaaagcg aaaggtgaaa
    11101 tctttagcgg cctgccggaa aacggtatcg ccattatgaa cgccgacaac aacgactggc
    11161 tgaactggca gagcgtaatt ggctcacgca aagtgtggcg tttctcaccc aatgccgcca
    11221 acagcgattt caccgccacc aatatccatg tgacctcgca cggtacggaa tttaccctac
    11281 aaaccccaac cggtagcgtc gatgttctgc tgccgttgcc ggggcgtcac aatattgcga
    11341 atgcgctggc agccgctgcg ctctccatgt ccgtgggcgc aacgcttgat gctatcaaag
    11401 cggggctggc aaatctgaaa gctgttccag gccgtctgtt ccccatccaa ctggcagaaa
    11461 accagttgct gctcgacgac tcctacaacg ccaatgtcgg ttcaatgacc gccgctgttc
    11521 aggtactggc tgaaatgccg ggctaccgcg tgctggtggt gggcgatatg gcggaactgg
    11581 gcgctgaaag cgaagcctgc catgtacagg tgggcgaggc ggcaaaagct gctggtattg
    11641 accgcgtgtt aagcgtgggt aaacaaagcc atgctatcag caccgccagc ggcgttggcg
    11701 aacattttgc tgataaaact gcgttaatta cgcgtcttaa atcactgatt gctgagcaac
    11761 aggtaattac gattttagtt aagggttcac gtagtgccgc catggaagag gtagtacgcg
    11821 ctttacagga gaatgggaca tgttagtttg gctggccgaa catttggtca aatattattc
    11881 cggctttaac gtcttttcct atctgacgtt tcgcgccatc gtcagcctgc tgaccgcgct
    11941 gttcatctca ttgtggatgg gcccgcgtat gattgctcat ttgcaaaaac tttcctttgg
    12001 tcaggtggtg cgtaacgacg gtcctgaatc acacttcagc aagcgcggta cgccgaccat
    12061 gggcgggatt atgatcctga cggcgattgt gatctccgta ctgctgtggg cttacccgtc
    12121 caatccgtac gtctggtgcg tgttggtggt gctggtaggt tacggtgtta ttggctttgt
    12181 tgatgattat cgcaaagtgg tgcgtaaaga caccaaaggg ttgatcgctc gttggaagta
    12241 tttctggatg tcggtcattg cgctgggtat cgccttcgcc ctgtaccttg ccggcaaaga
    12301 cacgcccgca acgcagctgg tggtcccatt ctttaaagat gtgatgccgc agctggggct
    12361 gttctacatt ctgctggctt acttcgtcat tgtgggtact ggcaacgcgg taaacctgac
    12421 cgatggtctc gacggcctgg caattatgcc gaccgtattt gtcgccggtg gttttgcgct
    12481 ggtggcgtgg gcgaccggca acatgaactt tgccagctat ttacacatac cgtatctgcg
    12541 acacgccggg gaactggtca ttgtctgcac ggcgatagtc ggggcaggac tgggcttcct
    12601 gtggtttaac acctatccgg cgcaggtctt tatgggcgat gtaggttcgc tggcgttagg
    12661 tggtgcgtta ggcattatcg ccgtactgct acgtcaggaa ttcctgctgg tgattatggg
    12721 gggcgtgttc gtggtagaaa cgctttctgt catcctgcag gtcggctcct ttaaactgcg
    12781 cggacaacgt attttccgca tggcaccgat tcatcaccac tatgaactga aaggctggcc
    12841 ggaaccgcgc gtcattgtgc gtttctggat tatttcgctg atgctggttc tgattggtct
    12901 ggcaacgctg aaggtacgtt aatcatggct gattatcagg gtaaaaatgt cgtcattatc
    12961 ggcctgggcc tcactgggct ttcctgcgtg gactttttcc tcgctcgcgg tgtgacgccg
    13021 cgcgttatgg atacgcgtat gacaccgcct ggcctggata aattacccga agccgtagaa
    13081 cgccacacgg gcagtctgaa tgatgaatgg ctgatggcgg cagatctgat tgtcgccagt
    13141 cccggtattg cactggcgca tccatcctta agcgctgccg ctgatgccgg aatcgaaatc
    13201 gttggcgata tcgagctgtt ctgccgcgaa gcacaagcac cgattgtggc gattaccggt
    13261 tctaacggca aaagcacggt caccacgcta gtgggtgaaa tggcgaaagc ggcgggggtt
    13321 aacgttggtg tgggtggcaa tattggcctg ccagcgttga tgctgcttga tgctgagtgc
    13381 gaactgtacg tgctggaact gtcgagcttc cagctggaaa ccacttccag cttacaggcg
    13441 gtggcggcga ccattctgaa cgtgactgaa gatcatatgg accgctatcc gtttggtttg
    13501 cagcaatacc gtgcggcaaa attgcgtatt tacgaaaatg cgaaagtttg tgtcgtcaat
    13561 gctgatgatg ccttaacaat gccgattcgc ggtgctgatg aacgctgcgt cagctttggc
    13621 gtcaacatgg gtgactatca cctgaatcat cagcagggag aaacctggct gcgagtgaaa
    13681 ggcgagaaag tgctgaacgt gaaagagatg aaactttccg ggcagcataa ctacaccaat
    13741 gcgctggcag cactggcact ggcagacgca gctggtttgc cgcgcgccag cagcctgaaa
    13801 gcgttaacca ccttcaccgg tctgccgcat cgctttgaag tggtgctgga gcacaatggc
    13861 gtacgctgga ttaacgattc gaaagccact aacgtcggca gtacggaagc ggcgttgaat
    13921 ggcttgcagg tagacggcac gctgcatttg ttgctgggcg gcgatggtaa atcggcggac
    13981 ttcagtccac tggcgcgcta tctgaatggc gacaacgtgc gtttgtactg ttttggtcgt
    14041 gacggtgcgc agctggcggc gctacgcccg gaagtggcag aacaaaccga aactatggaa
    14101 caggcgatgc gcttgctggc tccgcgtgtt cagccgggcg atatggttct gctctccccg
    14161 gcctgtgcca gccttgatca gttcaagaac tttgaacaac gaggcaatga gtttgcccgt
    14221 ctggcgaagg agttaggttg atgcgtttat ctctccctcg cctgaaaatg ccgcgcctgc
    14281 caggattcag tatcctggtc tggatctcca cggcgctaaa aggctgggtg atgggctcgc
    14341 gggaaaaaga taccgacagc ctgatcatgt acgatcgcac cttactgtgg ctgaccttcg
    14401 gccttgcggc gattggcttt atcatggtga cctcggcgtc aatgcccata gggcaacgct
    14461 taaccaacga tccgttcttc ttcgcgaagc gtgatggcgt ctatctgatc ctggcattca
    14521 ttctggcgat cattacgctg cgtctgccga tggagttctg gcaacgctac agtgccacga
    14581 tgctgctcgg atctatcatc ctgctgatga tcgtgctggt agtgggtagc tcggttaaag
    14641 gggcatcgcg ttggatcgat ctcggtttgc tgcgtatcca gcctgcggag ctgacaaaac
    14701 tgtcgctgtt ttgttatatc gccaactatc tggtgcgtaa aggcgacgaa gtacgtaata
    14761 acctgcgcgg cttcctgaaa ccgatgggcg tgattctggt gttggcagtg ttactgctgg
    14821 cacagccaga ccttggtacg gtggtggtgt tgtttgtgac tacgctggcg atgttgttcc
    14881 tggcgggagc gaaattgtgg cagttcattg ccattatcgg tatgggcatt tcagcggttg
    14941 tgttgctgat actcgccgaa ccgtaccgta tccgccgtgt taccgcattc tggaacccgt
    15001 gggaagatcc ctttggcagc ggctatcagt taacgcaatc gctgatggcg tttggtcgcg
    15061 gcgaactttg gggacaaggt ttaggtaact cggtacaaaa actggagtat ctgccggaag
    15121 cgcacactga ctttattttc gccattatcg gcgaagaact ggggtatgtc ggtgtggtgc
    15181 tggcactttt aatggtattc ttcgtcgctt ttcgcgcgat gtcgattggc cgcaaagcgt
    15241 tagaaattga ccaccgtttt tccggttttc tcgcctgttc tattggcatc tggtttagct
    15301 tccaggcgct ggttaacgta ggcgcggcgg cggggatgtt accgaccaaa ggtctgacat
    15361 tgccgctgat cagttacggt ggttcgagct tactgattat gtcgacagcc atcatgatgc
    15421 tgttgcgtat tgattatgaa acgcgtctgg agaaagcgca ggcgtttgta cgaggttcac
    15481 gatgagtggt caaggaaagc gattaatggt gatggcaggc ggaaccggtg gacatgtatt
    15541 cccgggactg gcggttgcgc accatctaat ggctcagggt tggcaagttc gctggctggg
    15601 gactgccgac cgtatggaag cggacttagt gccaaaacat ggcatcgaaa ttgatttcat
    15661 tcgtatctct ggtctgcgtg gaaaaggtat aaaagcactg atagctgcgc cgctgcgtat
    15721 cttcaacgcc tggcgtcagg cgcgggcgat tatgaaagcg tacaaacctg acgtggtact
    15781 cggtatgggc ggctatgtat caggtccagg tggtctggcc gcgtggtcgt taggcattcc
    15841 ggttgtactt catgaacaaa acggtattgc gggcttaacc aataaatggc tggcgaagat
    15901 tgccaccaaa gtgatgcagg cgtttccagg cgctttccct aatgcggaag tggtgggtaa
    15961 cccggtgcgt accgatgtac tggcgctgcc gttgccgcag caacgtttgg ctggacgtga
    16021 aggtccggtt cgtgtgctgg tagtgggtgg ttcccagggc gcacgcattc ttaaccagac
    16081 aatgccgcag gttgctgcga aactgggtga ttcagtcact atctggcatc agagcggtaa
    16141 aggttcgcaa caatccgttg aacaggcgta tgccgaagcg gggcaaccgc agcataaagt
    16201 gacggaattt attgatgata tggcggcggc gtatgcgtgg gcggatgtcg tcgtttgccg
    16261 ctccggtgct ttaacggtga gtgaaatcgc cgcggcagga ctaccggcgt tgtttgtgcc
    16321 gtttcaacat aaagaccgcc agcaatactg gaatgcgcta ccgctggaaa aagcgggcgc
    16381 agccaaaatt atcgagcagc cacagcttag cgtggatgct gtcgccaaca ccctggccgg
    16441 gtggtcgcga gaaaccttat taaccatggc agaacgcgcc cgcgctgcat ccattccgga
    16501 tgccaccgag cgagtggcaa atgaagtgag ccgggcagcc cgggcgtaat tgtagcgatg
    16561 ccttttgcat cgtatgaatt taagaagtta atggcgtaaa gaatgaatac acaacaattg
    16621 gcaaaactgc gttccatcgt gcccgaaatg cgtcgcgttc ggcacataca ttttgtcggc
    16681 atcggtggtg ccggtatggg cggtattgcc gaagttctgg ccaatgaagg ttatcagatc
    16741 agtggttccg atttagcgcc aaatccggtc acgcagcagt taatgaatct gggagcgacg
    16801 atttatttca accatcgccc ggaaaacgta cgtgatgcca gcgtggtcgt tgtttccagc
    16861 gccatttctg cctataaccc ggaaattgtc gcagctcatg aagcgcgtat tccggtgatc
    16921 cgtcgtgccg aaatgctggc tgagttaatg cgttttcgtc atggcatcgc cattgcagga
    16981 acgcacggca aaacgacaac caccgcgatg gtttccagca tctacgcaga agcggggctc
    17041 gacccaacct tcgttaacgg cgggctggta aaagcggcgg gggttcatgc gcgtttgggg
    17101 catggtcggt acctgattgc cgaagcagat gagagtgatg catcgttcct gcatctgcaa
    17161 ccgatggtgg cgattgtcac caatatcgaa gccgaccaca tggataccta ccagggcgac
    17221 tttgagaatt taaaacagac ttttattaat tttctgcaca acctgccgtt ttacggtcgt
    17281 gcggtgatgt gtgttgatga tccggtgatc cgcgaattgt taccgcgagt gggacgtcag
    17341 accacgactt acggcttcag cgaagatgcc gacgtgcgtg tagaagatta tcagcagatt
    17401 ggcccgcagg ggcactttac gctgctgcgc caggacaaag agccgatgcg cgtcaccctg
    17461 aatgcgccag gtcgtcataa cgcgctgaac gccgcagctg cggttgcggt tgctacggaa
    17521 gagggaattg atgacgaggc tattttacgt gcgctggaga gcttccaggg gactgggcgc
    17581 cgttttgatt tcctcggtga attcccgctg gagccagtga atggtaaaag cggtacggca
    17641 atgctggtcg atgactacgg ccaccacccg acggaagttg acgccaccat taaagcggcg
    17701 cgcgcaggct ggccggataa aaacctggta atgctgtttc agccgcaccg ttttacccgt
    17761 acgcgcgacc tgtatgatga tttcgccaat gtgctgacgc aggttgatac cctgttgatg
    17821 ctggaagtgt atccggctgg cgaagcgcca attccgggag cggacagccg ttcgctgtgt
    17881 cgcacaattc gtggacgtgg gaaaattgat cccattctgg tgccggaccc tgcgcaggta
    17941 gccgagatgc tggcaccggt attaaccggt aacgacctga ttctcgttca gggggctggt
    18001 aatatcggaa aaattgcccg ttctttagct gaaatcaaac tgaagccgca aactccggag
    18061 gaagaacaac atgactgata aaatcgcggt cctgttgggc gggacttccg ctgagcggga
    18121 agtttctctg aattctggcg cagcggtgtt agccgggctg cgtgaaggcg gtattgatgc
    18181 gtatcctgtc gacccgaaag aagtcgacgt gacgcaactg aagtcgatgg gttttcagaa
    18241 agtgtttatc gcgctacacg gtcgcggcgg tgaagatggt acgctgcagg gcatgctcga
    18301 gctgatgggc ttgccttata ccggcagcgg agtgatggca tctgcgcttt caatggataa
    18361 actacgcagc aaactgctgt ggcaaggtgc cggtttaccg gtcgcgccgt gggtagcgtt
    18421 aacccgcgca gagtttgaaa aaggcctgag cgataagcag ttagcagaaa tttctgctct
    18481 gggtttgccg gttatcgtta agccgagccg cgaaggttcc agtgtgggaa tgtcaaaagt
    18541 agtagcagaa aatgctctac aagatgcatt aagattggca tttcagcacg atgaagaagt
    18601 attgattgaa aaatggctaa gtgggccgga gttcacggtt gcgatactcg gtgaagaaat
    18661 tttaccgtca atacgtattc aaccgtccgg aaccttctat gattatgagg cgaagtatct
    18721 ctctgatgag acacagtatt tctgccccgc aggtctggaa gcgtcacaag aggccaattt
    18781 gcaggcatta gtgctgaaag catggacgac gttaggttgc aaaggatggg gacgtattga
    18841 cgttatgctg gacagcgatg gacagtttta tctgctggaa gccaatacct caccgggtat
    18901 gaccagccac agcctggtgc cgatggcggc acgtcaggca ggtatgagct tctcgcagtt
    18961 ggtagtacga attctggaac tggcggacta atatgtcgca ggctgctctg aacacgcgaa
    19021 atagcgaaga agaggtttct tctcgccgca ataatggaac gcgtctggcg gggatccttt
    19081 tcctgctgac cgttttaacg acagtgttgg tgagcggctg ggtcgtgttg ggctggatgg
    19141 aagatgcgca acgcctgccg ctctcaaagc tggtgttgac cggtgaacgc cattacacgc
    19201 gtaatgacga tatccggcag tcgatcctgg cattgggtga gccgggtacc tttatgaccc
    19261 aagatgtcaa catcatccag acgcaaatag aacaacgcct gccgtggatt aagcaggtga
    19321 gcgtcagaaa gcagtggcct gatgaattga agattcatct ggttgaatat gtgccgattg
    19381 cgcggtggaa tgatcaacat atggtagacg cggaaggaaa caccttcagc gtgccgccag
    19441 atcgcaccag caagcaggtg cttccaatgc tgtatggccc ggaaggcagc gccaatgaag
    19501 tgttgcaggg ctatcgcgaa atggggcaga tgctggcaaa ggacagattt actctgaagg
    19561 aagcggcgat gaccgcgcgg cgttcctggc agttgacgct gaataacgat attaagctca
    19621 atcttggccg gggcgatacg atgaaacgtt tggctcgctt tgtagaactt tatccggttt
    19681 tacagcagca ggcgcaaacc gatggcaaac ggattagcta cgttgatttg cgttatgact
    19741 ctggagcggc agtaggctgg gcgcccttgc cgccagagga atctactcag caacaaaatc
    19801 atgcacaggc agaacaacaa tgatcaaggc gacggacaga aaactggtag taggactgga
    19861 gattggtacc gcgaaggttg ccgctttagt aggggaagtt ctgcccgacg gtatggtcaa
    19921 tatcattggc gtgggcagct gcccgtcacg tggtatggat aaaggcgggg tgaacgacct
    19981 cgaatccgtg gtcaagtgcg tacaacgcgc cattgaccag gcagaattga tggcagattg
    20041 tcagatctct tcggtatatc tggcgctttc tggtaagcac atcagctgcc agaatgaaat
    20101 tggtatggtg cctatttctg aagaagaagt gacgcaagaa gatgtggaaa acgtcgtcca
    20161 taccgcgaaa tcggtgcgtg tgcgcgatga gcatcgtgtg ctgcatgtga tcccgcaaga
    20221 gtatgcgatt gactatcagg aagggatcaa gaatccggta ggactttcgg gcgtgcggat
    20281 gcaggcaaaa gtgcacctga tcacatgtca caacgatatg gcgaaaaaca tcgtcaaagc
    20341 ggttgaacgt tgtgggctga aagttgacca actgatattt gccggactgg catcaagtta
    20401 ttcggtattg acggaagatg aacgtgaact gggtgtctgc gtcgtcgata tcggtggtgg
    20461 tacaatggat atcgccgttt ataccggtgg ggcattgcgc cacactaagg taattcctta
    20521 tgctggcaat gtcgtgacca gtgatatcgc ttacgccttt ggcacgccgc caagcgatgc
    20581 cgaagcgatt aaagttcgcc acggttgtgc gctgggttcc atcgttggaa aagatgagag
    20641 cgtggaagtg ccgagcgtag gtggtcgtcc gccgcggagt ctgcaacgtc agaccctggc
    20701 agaggtgatc gagccgcgct ataccgagct gctcaacctg gtcaacgaag agatattgca
    20761 gttgcaggaa aagcttcgcc aacaaggggt taaacatcac ctggcggcag gcattgtatt
    20821 aaccggtggc gcagcgcaga tcgaaggtct tgcagcctgt gctcagcgcg tgtttcatac
    20881 gcaagtgcgt atcggcgcgc cgctgaacat taccggttta acggattatg ctcaggagcc
    20941 gtattattcg acggcggtgg gattgcttca ctatgggaaa gagtcacatc ttaacggtga
    21001 agctgaagta gaaaaacgtg ttacagcatc agttggctcg tggatcaagc gactcaatag
    21061 ttggctgcga aaagagtttt aatttttatg aggccgacga tgattacggc ctcaggcgac
    21121 aggcacaaat cggagagaaa ctatgtttga accaatggaa cttaccaatg acgcggtgat
    21181 taaagtcatc ggcgtcggcg gcggcggcgg taatgctgtt gaacacatgg tgcgcgagcg
    21241 cattgaaggt gttgaattct tcgcggtaaa taccgatgca caagcgctgc gtaaaacagc
    21301 ggttggacag acgattcaaa tcggtagcgg tatcaccaaa ggactgggcg ctggcgctaa
    21361 tccagaagtt ggccgcaatg cggctgatga ggatcgcgat gcattgcgtg cggcgctgga
    21421 aggtgcagac atggtcttta ttgctgcggg tatgggtggt ggtaccggta caggtgcagc
    21481 accagtcgtc gctgaagtgg caaaagattt gggtatcctg accgttgctg tcgtcactaa
    21541 gcctttcaac tttgaaggca agaagcgtat ggcattcgcg gagcagggga tcactgaact
    21601 gtccaagcat gtggactctc tgatcactat cccgaacgac aaactgctga aagttctggg
    21661 ccgcggtatc tccctgctgg atgcgtttgg cgcagcgaac gatgtactga aaggcgctgt
    21721 gcaaggtatc gctgaactga ttactcgtcc gggtttgatg aacgtggact ttgcagacgt
    21781 acgcaccgta atgtctgaga tgggctacgc aatgatgggt tctggcgtgg cgagcggtga
    21841 agaccgtgcg gaagaagctg ctgaaatggc tatctcttct ccgctgctgg aagatatcga
    21901 cctgtctggc gcgcgcggcg tgctggttaa catcacggcg ggcttcgacc tgcgtctgga
    21961 tgagttcgaa acggtaggta acaccatccg tgcatttgct tccgacaacg cgactgtggt
    22021 tatcggtact tctcttgacc cggatatgaa tgacgagctg cgcgtaaccg ttgttgcgac
    22081 aggtatcggc atggacaaac gtcctgaaat cactctggtg accaataagc aggttcagca
    22141 gccagtgatg gatcgctacc agcagcatgg gatggctccg ctgacccagg agcagaagcc
    22201 ggttgctaaa gtcgtgaatg acaatgcgcc gcaaactgcg aaagagccgg attatctgga
    22261 tatcccagca ttcctgcgta agcaagctga ttaagaattg actggaattt gggtttcgag
    22321 gctctttgtg ctaaactggc ccgccgaatg tatagtacac ttcggttgga taggtaattt
    22381 ggcgagataa tacgatgatc aaacaaagga cacttaaacg tatcgttcag gcgacgggtg
    22441 tcggtttaca taccggcaag aaagtcaccc tgacgttacg ccctgcgccg gccaacaccg
    22501 gggtcatcta tcgtcgcacc gacttgaatc caccggtaga tttcccggcc gatgccaaat
    22561 ctgtgcgtga taccatgctc tgtacgtgtc tggtcaacga gcatgatgta cggatttcaa
    22621 ccgtagagca cctcaatgct gctctcgcgg gcttgggcat cgataacatt gttatcgaag
    22681 ttaacgcgcc ggaaatcccg atcatggacg gcagcgccgc tccgtttgta tacctgctgc
    22741 ttgacgccgg tatcgacgag ttgaactgcg ccaaaaaatt tgttcgcatc aaagagactg
    22801 ttcgtgtcga agatggcgat aagtgggctg aatttaagcc gtacaatggt ttttcgctgg
    22861 atttcaccat cgattttaac catccggcta ttgattccag caaccagcgc tatgcgatga
    22921 acttctccgc tgatgcgttt atgcgccaga tcagccgtgc gcgtacgttc ggtttcatgc
    22981 gtgatatcga atatctgcag tcccgtggtt tgtgcctggg cggcagcttc gattgtgcca
    23041 tcgttgttga cgattatcgc gtactgaacg aagacggcct gcgttttgaa gacgaatttg
    23101 tgcgtcacaa aatgctcgat gcgatcggtg acttgttcat gtgtggtcac aatattattg
    23161 gtgcatttac cgcttataaa tccggtcatg cactgaataa caaactgctg caggctgtcc
    23221 tggcgaaaca ggaagcctgg gaatatgtga ccttccagga cgacgcagaa ctgccgttgg
    23281 ccttcaaagc gccttcagct gtactggcat aacgacattt atactgtcgt ataaaattcg
    23341 actggcaaat ctggcactct ct
//
LOCUS       DAZKHF020000052        23206 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2201S31241
            SAMN43267800-rid25106413.denovo.052, whole genome shotgun sequence.
ACCESSION   DAZKHF020000052 DAZKHF020000000
VERSION     DAZKHF020000052.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267800
            Sequence Read Archive: SRR30307263
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 23206)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23206)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 23206)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350581.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350581.2
            Genome Coverage       :: 151x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:21:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,652
            CDSs (total)                      :: 5,557
            Genes (coding)                    :: 5,320
            CDSs (with protein)               :: 5,320
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 237
            CDSs (without protein)            :: 237
            Pseudo Genes (ambiguous residues) :: 0 of 237
            Pseudo Genes (frameshifted)       :: 102 of 237
            Pseudo Genes (incomplete)         :: 130 of 237
            Pseudo Genes (internal stop)      :: 52 of 237
            Pseudo Genes (multiple problems)  :: 41 of 237
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23206
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267800-rid25106413.denovo.052"
                     /serotype="O 26:H11"
                     /isolate="2201S31241"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-01"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            123..1487
                     /locus_tag="ACHOSC_RS08220"
                     /old_locus_tag="pgaptmp_001644"
     CDS             123..1487
                     /locus_tag="ACHOSC_RS08220"
                     /old_locus_tag="pgaptmp_001644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414961.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HGU4547606.1"
                     /translation="MNDYKMTPGERRATWGLGTVFSLRMLGMFMVLPVLTTYGMALQG
                     ASEALIGIAIGIYGLTQAVFQIPFGLLSDRIGRKPLIVGGLAVFAAGSVIAALSDSIW
                     GIILGRALQGSGAIAAAVMALLSDLTREQNRTKAMAFIGVSFGITFAIAMVLGPIITH
                     KLGLHALFWMIAILATTGIALTIWVVPNSSTHVLNRESGMVKGSFSKVLAEPRLLKLN
                     FGIMCLHMLLMSTFVALPGQLADAGFPAAEHWKVYLATMLIAFGSVVPFIIYAEVKRK
                     MKQVFVFCVGLIVVAEIVLWNAQTQFWQLVVGVQLFFVAFNLMEALLPSLISKESPAG
                     YKGTAMGVYSTSQFLGVAIGGSLGGWIDGMFDGQGVFLAGAMLAAVWLAVASTMKEPP
                     YVSSLRIEIPADIAANEALKVRLLETEGVKEVLIAEEEHSAYVKIDSKVTNRFEIEQA
                     IRQA"
     gene            complement(1615..2106)
                     /gene="yajQ"
                     /locus_tag="ACHOSC_RS08225"
                     /old_locus_tag="pgaptmp_001645"
     CDS             complement(1615..2106)
                     /gene="yajQ"
                     /locus_tag="ACHOSC_RS08225"
                     /old_locus_tag="pgaptmp_001645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414960.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide binding protein YajQ"
                     /protein_id="HGU4547607.1"
                     /translation="MPSFDIVSEVDLQEARNAVDNASREVESRFDFRNVEASFELNDA
                     SKTIKVLSESDFQVNQLLDILRAKLLKRGIEGSSLDVPENIVHSGKTWFVEAKLKQGI
                     ESATQKKIVKMIKDSKLKVQAQIQGDEIRVTGKSRDDLQAVMAMVRGGDLGQPFQFKN
                     FRD"
     gene            2274..3185
                     /gene="panE"
                     /locus_tag="ACHOSC_RS08230"
                     /old_locus_tag="pgaptmp_001646"
     CDS             2274..3185
                     /gene="panE"
                     /locus_tag="ACHOSC_RS08230"
                     /old_locus_tag="pgaptmp_001646"
                     /EC_number="1.1.1.169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008677 - 2-dehydropantoate 2-reductase
                     activity [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydropantoate 2-reductase"
                     /protein_id="HGU4547608.1"
                     /translation="MKITVLGCGALGQLWLTALCKQGHEVQGWLRVPQPYCSVNLVET
                     DGSIFNESLTANDPDFLATSDLLLVTLKAWQVYDAVKSLASTLPVTTPILLIHNGMGT
                     IEELQNIQQPLLMGTTTHAARRDGNVIIHVANGITHIGPARQQDGDYSYLADILQTVL
                     PDVAWHNNIRAELWRKLAVNCVINPLTAIWNCPNGELRHHPQEIMQICEEVAAVIERE
                     GHHTSAEDLRDYVMQVIDATAENISSMLQDIRALRHTEIDYINGFLLRRARAHGIAVP
                     ENTRLFEMVKRKESEYERIGTGLPRPW"
     gene            3148..3738
                     /gene="yajL"
                     /locus_tag="ACHOSC_RS08235"
                     /old_locus_tag="pgaptmp_001647"
     CDS             3148..3738
                     /gene="yajL"
                     /locus_tag="ACHOSC_RS08235"
                     /old_locus_tag="pgaptmp_001647"
                     /EC_number="3.5.1.124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308505.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein deglycase YajL"
                     /protein_id="HGU4547609.1"
                     /translation="MSASALVCLAPGSEETEAVTTIDLLVRGGIKVTTASVASDGNLA
                     ITCSRGVKLLADAPLVEVADGEYDVIVLPGGIKGAECFRDSTLLVETVKQFHRSGRIV
                     AAICAAPATVLVPHDIFPIGNMTGFPTLKDKIPAEQWQDKRVVWDARVKLLTSQGPGT
                     AIDFGLKIIDLLVGREKAHEVASQLVMAAGIYNYYE"
     gene            complement(3792..5240)
                     /gene="thiI"
                     /locus_tag="ACHOSC_RS08240"
                     /old_locus_tag="pgaptmp_001648"
     CDS             complement(3792..5240)
                     /gene="thiI"
                     /locus_tag="ACHOSC_RS08240"
                     /old_locus_tag="pgaptmp_001648"
                     /EC_number="2.8.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014168857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0140741 - tRNA-uracil-4 sulfurtransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uracil 4-sulfurtransferase ThiI"
                     /protein_id="HGU4547610.1"
                     /translation="MKFIIKLFPEITIKSQSVRLRFIKILTGNIRNVLKHYDETLAVV
                     RHWDNIEVRAKDENQRLAIRDALTRIPGIHHILEVEDVPFTDMHDIFEKALVQYRDQL
                     EGKTFCVRVKRRGKHDFSSIDVERYVGGGLNQHIESARVKLTNPEVTVHLEVEDDRLL
                     LIKGRYEGIGGFPIGTQEDVLSLISGGFDSGVSSYMLMRRGCRVHYCFFNLGGAAHEI
                     GVRQVAHYLWNRFGSSHRVRFVAINFEPVVGEILEKIDDGQMGVILKRMMVRAASKVA
                     ERYGVQALVTGEALGQVSSQTLTNLRLIDNVSDTLILRPLISYDKEHIINLARQIGTE
                     DFARTMPEYCGVISKSPTVKAVKSKIEAEEEKFDFSILDKVVEEANNVDIREIAQQTE
                     QEVVEVETVNGFGPNDVILDIRSIDEQEDKPLKVEGIDVVSLPFYKLSTKFGDLDQNR
                     TWLLWCERGVMSRLQALYLREQGFNNVKVYRP"
     gene            5446..5688
                     /gene="xseB"
                     /locus_tag="ACHOSC_RS08245"
                     /old_locus_tag="pgaptmp_001649"
     CDS             5446..5688
                     /gene="xseB"
                     /locus_tag="ACHOSC_RS08245"
                     /old_locus_tag="pgaptmp_001649"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII small subunit"
                     /protein_id="HGU4547611.1"
                     /translation="MPKKNEAPASFEKALSELEQIVTRLESGDLPLEEALNEFERGVQ
                     LARQGQAKLQQAEQRVQILLSDNEDASLTPFTPDNE"
     gene            5688..6587
                     /gene="ispA"
                     /locus_tag="ACHOSC_RS08250"
                     /old_locus_tag="pgaptmp_001650"
     CDS             5688..6587
                     /gene="ispA"
                     /locus_tag="ACHOSC_RS08250"
                     /old_locus_tag="pgaptmp_001650"
                     /EC_number="2.5.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(2E,6E)-farnesyl diphosphate synthase"
                     /protein_id="HGU4547612.1"
                     /translation="MDFPQQLEACVKQANQALSRFIAPLPFQNTPVVETMQYGALLGG
                     KRLRPFLVYATGHMFGVSTNTLDAPAAAVECIHAYSLIHDDLPAMDDDDLRRGLPTCH
                     VKFGEANAILAGDALQTLAFSILSDANMPEVSDRDRISMISELASASGIAGMCGGQAL
                     DLDAEGKHVPLDALERIHRHKTGALIRAAVRLGALSAGDKGRRALPVLDKYAESIGLA
                     FQVQDDILDVVGDTATLGKRQGADQQLGKSTYPALLGLEQARKKARDLIDDARQSLKQ
                     LAEQSLDTSALEALADYIIQRNK"
     gene            6612..8474
                     /gene="dxs"
                     /locus_tag="ACHOSC_RS08255"
                     /old_locus_tag="pgaptmp_001651"
     CDS             6612..8474
                     /gene="dxs"
                     /locus_tag="ACHOSC_RS08255"
                     /old_locus_tag="pgaptmp_001651"
                     /EC_number="2.2.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008661 - 1-deoxy-D-xylulose-5-phosphate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009240 - isopentenyl diphosphate
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate synthase"
                     /protein_id="HGU4547613.1"
                     /translation="MSFDIAKYPTLALVDSTQELRLLPKESLPKLCDELRRYLLDSVS
                     RSSGHFASGLGTVELTVALHYVYNTPFDQLIWDVGHQAYPHKILTGRRDKIGTIRQKG
                     GLHPFPWRGESEYDVLSVGHSSTSISAGIGIAVAAEKEGKNRRTVCVIGDGAITAGMA
                     FEAMNHAGDIRPDMLVVLNDNEMSISENVGALNNHLAQLLSGKLYSSLREGGKKVFSG
                     VPPIKELLKRTEEHIKGMVVPGTLFEELGFNYIGPVDGHDVLGLITTLKNMRDLKGPQ
                     FLHIMTKKGRGYEPAEKDPITFHAVPKFDPSSGCLPKSSGGLPSYSKIFGDWLCETAA
                     KDNKLMAITPAMREGSGMVEFSRKFPDRYFDVAIAEQHAVTFAAGLAIGGYKPIVAIY
                     STFLQRAYDQVLHDVAIQKLPVLFAIDRAGIVGADGQTHQGAFDLSYLRCIPEMVIMT
                     PSDENECRQMLYTGYHYNDGPSAVRYPRGNAVGMELTPLEKLPIGKGIVKRRGEKLAI
                     LNFGTLMPDAAKVAESLNATLVDMRFVKPLDEALILEMAASHEALVTVEENAIMGGAG
                     SGVNEVLMAHRKPVPVLNIGLPDFFIPQGTQEEMRAELGLDAAGMEAKIKAWLA"
     gene            8654..9628
                     /gene="yajO"
                     /locus_tag="ACHOSC_RS08260"
                     /old_locus_tag="pgaptmp_001652"
     CDS             8654..9628
                     /gene="yajO"
                     /locus_tag="ACHOSC_RS08260"
                     /old_locus_tag="pgaptmp_001652"
                     /EC_number="1.17.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414953.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxyxylulose-5-phosphate synthase YajO"
                     /protein_id="HGU4547614.1"
                     /translation="MQYNPLGKTDLRVSRLCLGCMTFGEPDRGNHAWTLPEESSRPII
                     KRALEGGINFFDTANSYSDGSSEEIVGRALRDFARREDVVVATKVFHRVGDLPEGLSR
                     AQILRSIDDSLRRLGMDYVDILQIHRWDYNTPIEETLEALNDVVKAGKARYIGASSMH
                     ASQFAQALKLQKQHGWAQFVSMQDHYNLIYREEESEMLPLCYQEGVAVIPWSPLARGR
                     LTRPWGETTARLVSDEVGKNLYKESDENDAQIAERLTGVSEELGATRAQVALAWLLSK
                     PGIAAPIIGTSREEQLDELLNAVDITLKPEQIAELETPYKPHAVVGFK"
     gene            complement(9682..10200)
                     /gene="pgpA"
                     /locus_tag="ACHOSC_RS08265"
                     /old_locus_tag="pgaptmp_001653"
     CDS             complement(9682..10200)
                     /gene="pgpA"
                     /locus_tag="ACHOSC_RS08265"
                     /old_locus_tag="pgaptmp_001653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008962 - phosphatidylglycerophosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylglycerophosphatase A"
                     /protein_id="HGU4547615.1"
                     /translation="MTILPRHKDVAKSRLKMSNPWHLLAVGFGSGLSPIVPGTMGSLA
                     AIPFWYLMTFLPWQLYSLVVMLGICIGVYLCHQTAKDMGVHDHGSIVWDEFIGMWITL
                     MALPTNDWQWVAAGFVIFRILDMWKPWPIRWFDRNVHGGMGIMIDDIVAGVISAGILY
                     FIGHHWPLGILS"
     gene            complement(10178..11155)
                     /gene="thiL"
                     /locus_tag="ACHOSC_RS08270"
                     /old_locus_tag="pgaptmp_001654"
     CDS             complement(10178..11155)
                     /gene="thiL"
                     /locus_tag="ACHOSC_RS08270"
                     /old_locus_tag="pgaptmp_001654"
                     /EC_number="2.7.4.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009030 - thiamine-phosphate kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine-phosphate kinase"
                     /protein_id="HGU4547616.1"
                     /translation="MACGEFSLIARYFDRVRSSRLDVELGIGDDCALLNIPEKQTLAI
                     STDTLVAGNHFLHDIDPADLAYKALAVNLSDLAAMGADPAWLTLALTLPDVDEAWLES
                     FSDSLFDLLNYYDMQLIGGDTTRGPLSMTLGIHGFVPMGRALTRSGAKPGDWIYVTGT
                     PGDSAAGLAILQNRLQVADAKDADYLIKRHLRPSPRILQGQALRDLANSAIDLSDGLI
                     SDLGHIVKASDCGARIDLALLPFSDALSRHVEPEQALRWALSGGEDYELCFTVPELNR
                     GALDVALGHLGVPFTCIGQMTADIEGLCFIRDGEPVTFDWKGYDHFATP"
     gene            complement(11233..11652)
                     /gene="nusB"
                     /locus_tag="ACHOSC_RS08275"
                     /old_locus_tag="pgaptmp_001655"
     CDS             complement(11233..11652)
                     /gene="nusB"
                     /locus_tag="ACHOSC_RS08275"
                     /old_locus_tag="pgaptmp_001655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antitermination factor NusB"
                     /protein_id="HGU4547617.1"
                     /translation="MKPAARRRARECAVQALYSWQLSQNDIADVEYQFLAEQDVKDVD
                     VLYFRELLAGVATNTAYLDGLMKPYLSRLLEELGQVEKAVLRIALYELSKRSDVPYKV
                     AINEAIELAKSFGAEDSHKFVNGVLDKAAPVIRPNKK"
     gene            complement(11672..12142)
                     /gene="ribE"
                     /locus_tag="ACHOSC_RS08280"
                     /old_locus_tag="pgaptmp_001656"
     CDS             complement(11672..12142)
                     /gene="ribE"
                     /locus_tag="ACHOSC_RS08280"
                     /old_locus_tag="pgaptmp_001656"
                     /EC_number="2.5.1.78"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006818760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009349 - riboflavin synthase complex
                     [Evidence IEA];
                     GO_function: GO:0000906 - 6,7-dimethyl-8-ribityllumazine
                     synthase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6,7-dimethyl-8-ribityllumazine synthase"
                     /protein_id="HGU4547618.1"
                     /translation="MNIIEANVATPDARVAITIARFNNFINDSLLEGAIDALKRIGQV
                     KDENITVVWVPGAYELPLAAGALAKTGKYDAVIALGTVIRGGTAHFEYVAGGASNGLA
                     HVAQDSEIPVAFGVLTTESIEQAIERAGTKAGNKGAEAALTALEMINVLKAIKA"
     gene            complement(12231..13334)
                     /gene="ribD"
                     /locus_tag="ACHOSC_RS08285"
                     /old_locus_tag="pgaptmp_001657"
     CDS             complement(12231..13334)
                     /gene="ribD"
                     /locus_tag="ACHOSC_RS08285"
                     /old_locus_tag="pgaptmp_001657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0008703 -
                     5-amino-6-(5-phosphoribosylamino)uracil reductase activity
                     [Evidence IEA];
                     GO_function: GO:0008835 -
                     diaminohydroxyphosphoribosylaminopyrimidine deaminase
                     activity [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     diaminohydroxyphosphoribosylaminopyrimidine
                     deaminase/5-amino-6-(5-phosphoribosylamino)uracil
                     reductase RibD"
                     /protein_id="HGU4547619.1"
                     /translation="MQDEYYMARALKLAQRGRFTTHPNPNVGCVIVKDGEIVGEGYHQ
                     RAGEPHAEVHALRMAGEKAKGATAYVTLEPCSHHGRTPPCCDALIAAGVARVVASMQD
                     PNPQVAGRGLYRLQQAGIDVSHGLMMSEAEQLNKGFLKRMRTGFPYIQLKLGASLDGR
                     TAMASGESQWITSPQARRDVQRQRAQSHAILTSSATVLADDPALTVRWSELDEQTQAL
                     YPQQNLRQPVRIVIDSQNRVTPEHRIVQQPGETWFARTQEDSSEWPETVRTLLIPEHK
                     GHLDLVVLMMQLGKQQINSIWVEAGPTLAGALLQAGLVDELIVYIAPKLLGSDARGLC
                     TLPGLEKLADAPQFKFKEIRHVGPDVCLHLVGA"
     gene            complement(13338..13787)
                     /gene="nrdR"
                     /locus_tag="ACHOSC_RS08290"
                     /old_locus_tag="pgaptmp_001658"
     CDS             complement(13338..13787)
                     /gene="nrdR"
                     /locus_tag="ACHOSC_RS08290"
                     /old_locus_tag="pgaptmp_001658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017458860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0140110 - transcription regulator activity
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator NrdR"
                     /protein_id="HGU4547620.1"
                     /translation="MHCPFCFAVDTKVIDSRLVGEGSSVRRRRQCLVCNERFTTFEVA
                     ELVMPRVVKSNDVREPFNEEKLRSGMLRALEKRPVSSDDVEMAINHIKSQLRATGERE
                     VPSKMIGNLVMEQLKKLDKVAYIRFASVYRSFEDIKEFGEEIARLED"
     gene            13938..14477
                     /locus_tag="ACHOSC_RS08295"
                     /old_locus_tag="pgaptmp_001659"
     CDS             13938..14477
                     /locus_tag="ACHOSC_RS08295"
                     /old_locus_tag="pgaptmp_001659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414946.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3251 domain-containing protein"
                     /protein_id="HGU4547621.1"
                     /translation="MNTNVFRLLLLGSLFSLSACVQQSEVRQMKHSVSTLNQEMTQLN
                     KETVKITQQNRLNAKSSSGVYLLPGAKTPARLESQIGTLRMSLVNITPDADGTTLTLR
                     IQGESNDPLPAFSGTVEYGQIQGTIDNFQEINVQNQLINAPASVLAPSDVDIPLQLKG
                     ISVEQLDFVRIHDIQPVMQ"
     gene            14776..15654
                     /gene="tsx"
                     /locus_tag="ACHOSC_RS08300"
                     /old_locus_tag="pgaptmp_001660"
     CDS             14776..15654
                     /gene="tsx"
                     /locus_tag="ACHOSC_RS08300"
                     /old_locus_tag="pgaptmp_001660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-specific channel-forming protein Tsx"
                     /protein_id="HGU4547622.1"
                     /translation="MKKTLLAAGAVLALSSSFTVNAAENDKPQYLSDWWHQSVNVVGS
                     YHTRFGPQIRNDTYLEYEAFAKKDWFDFYGYADAPVFFGGNSDAKGIWNHGSPLFMEI
                     EPRFSIDKLTNTDLSFGPFKEWYFANNYIYDMGRNKDGRQSTWYMGLGTDIDTGLPMS
                     LSMNVYAKYQWQNYGAANENEWDGYRFKIKYFVPITDLWGGQLSYIGFTNFDWGSDLG
                     DDSGNAINGIKTRTNNSIASSHILALNYDHWHYSVVARYWHDGGQWNDDAELNFGNFN
                     VRSTGWGGYLVVGYNF"
     gene            complement(15831..16178)
                     /gene="yajD"
                     /locus_tag="ACHOSC_RS08305"
                     /old_locus_tag="pgaptmp_001661"
     CDS             complement(15831..16178)
                     /gene="yajD"
                     /locus_tag="ACHOSC_RS08305"
                     /old_locus_tag="pgaptmp_001661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH nuclease YajD"
                     /protein_id="HGU4547623.1"
                     /translation="MAIIPKNYARLESGYREKALKIYPWVCGRCSREFVYSNLRELTV
                     HHIDHDHTNNPEDGSNWELLCLYCHDHEHSKYTEADQYGTTVIAGEDAQKDVGEAKYN
                     PFADLKAMMNKKK"
     gene            complement(16307..17278)
                     /gene="secF"
                     /locus_tag="ACHOSC_RS08310"
                     /old_locus_tag="pgaptmp_001662"
     CDS             complement(16307..17278)
                     /gene="secF"
                     /locus_tag="ACHOSC_RS08310"
                     /old_locus_tag="pgaptmp_001662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031522 - cell envelope Sec protein
                     transport complex [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0006886 - intracellular protein transport
                     [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein translocase subunit SecF"
                     /protein_id="HGU4547624.1"
                     /translation="MAQEYTVEQLNHGRKVYDFMRWDYWAFGISGLLLIAAIVIMGVR
                     GFNWGLDFTGGTVIEITLEKPAEIDVMRDALQKAGFEEPMLQNFGSSHDIMVRMPPAE
                     GETGGQVLGSQVLKVINESTNQNAAVKRIEFVGPSVGADLAQTGAMALMAALLSILVY
                     VGFRFEWRLAAGVVIALAHDVIITLGILSLFHIEIDLTIVASLMSVIGYSLNDSIVVS
                     DRIRENFRKIRRGTPYEIFNVSLTQTLHRTLITSGTTLMVILMLYLFGGPVLEGFSLT
                     MLIGVSIGTASSIYVASALALKLGMKREHMLQQKVEKEGADQPSILP"
     gene            complement(17289..19136)
                     /gene="secD"
                     /locus_tag="ACHOSC_RS08315"
                     /old_locus_tag="pgaptmp_001663"
     CDS             complement(17289..19136)
                     /gene="secD"
                     /locus_tag="ACHOSC_RS08315"
                     /old_locus_tag="pgaptmp_001663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005125127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031522 - cell envelope Sec protein
                     transport complex [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0006886 - intracellular protein transport
                     [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein translocase subunit SecD"
                     /protein_id="HGU4547625.1"
                     /translation="MLNRYPLWKYVMLIVVIVIGLLYALPNLFGEDPAVQITGARGVA
                     ASEQTLIQVQKTLQEEKITAKSVALEEGAILARFDSTDTQLRAREALMGVMGDKYVVA
                     LNLAPATPRWLAAIHAEPMKLGLDLRGGVHFLMEVDMDTALGKLQEQNIDSLRSDLRE
                     KGIPYTTVRKENNYGLSITFRDAKARDEAIAYLSKRHPDLVISSQGSNQLRAVMSDAR
                     LSEAREYAVQQNINILRNRVNQLGVAEPVVQRQGADRIVVELPGIQDTARAKEILGAT
                     ATLEFRLVNTNVDQAAAASGRVPGDSEVKQTREGQPVVLYKRVILTGDHITDSTSSQD
                     EYNQPQVNISLDSAGGNIMSNFTKDNIGKPMATLFVEYKDSGKKDANGRAVLVKQEEV
                     INIANIQSRLGNSFRITGINNPNEARQLSLLLRAGALIAPIQIVEERTIGPTLGMQNI
                     EQGLEACLAGLLVSILFMIIFYKKFGLIATSALIANLILIVGIMSLLPGATLSMPGIA
                     GIVLTLAVAVDANVLINERIKEELSNGRTVQQAIDEGYRGAFSSIFDANITTLIKVII
                     LYAVGTGAIKGFAITTGIGVATSMFTAIVGTRAIVNLLYGGKRVKKLSI"
     gene            complement(19164..19496)
                     /gene="yajC"
                     /locus_tag="ACHOSC_RS08320"
                     /old_locus_tag="pgaptmp_001664"
     CDS             complement(19164..19496)
                     /gene="yajC"
                     /locus_tag="ACHOSC_RS08320"
                     /old_locus_tag="pgaptmp_001664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000007628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit YajC"
                     /protein_id="HGU4547626.1"
                     /translation="MSFFISDAVAATGAPAQGSPMSLILMLVVFGLIFYFMILRPQQK
                     RTKEHKKLMDSIAKGDEVLTNGGLVGRVTKVAENGYIAIALNDTTEVVIKRDFVAAVL
                     PKGTMKAL"
     gene            complement(19519..20646)
                     /gene="tgt"
                     /locus_tag="ACHOSC_RS08325"
                     /old_locus_tag="pgaptmp_001665"
     CDS             complement(19519..20646)
                     /gene="tgt"
                     /locus_tag="ACHOSC_RS08325"
                     /old_locus_tag="pgaptmp_001665"
                     /EC_number="2.4.2.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012904808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008479 - tRNA-guanosine(34) queuine
                     transglycosylase activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA guanosine(34) transglycosylase Tgt"
                     /protein_id="HGU4547627.1"
                     /translation="MKFELDTTDGRARRGRLVFDCGVVETPCFMPVGTYGTVKGMTPE
                     EVEATGAQIILGNTFHLWLRPGQEIMKLHGDLHDFMQWKGPILTDSGGFQVFSLGDIR
                     KITEQGVHFRNPINGDPIFLDPEKSMEIQYDLGSDIVMIFDECTPYPADWDYAKRSME
                     MSLRWAKRSRERFDSLGNKNALFGIIQGSVYEDLRDISVKGLVDIGFDGYAVGGLAVG
                     EPKADMHRILEHVCPQIPADKPRYLMGVGKPEDLVEGVRRGIDMFDCVMPTRNARNGH
                     LFVTDGVVKIRNAKYKSDTGPLDPECDCYTCRNYSRAYLHHLDRCNEILGARLNTIHN
                     LRYYQRLMAGLRKAIEEGKLESFVTDFYQRQGREVPPLNVD"
     gene            complement(20702..21772)
                     /gene="queA"
                     /locus_tag="ACHOSC_RS08330"
                     /old_locus_tag="pgaptmp_001666"
     CDS             complement(20702..21772)
                     /gene="queA"
                     /locus_tag="ACHOSC_RS08330"
                     /old_locus_tag="pgaptmp_001666"
                     /EC_number="2.4.99.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0051075 - S-adenosylmethionine:tRNA
                     ribosyltransferase-isomerase activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA];
                     GO_process: GO:0008616 - queuosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA preQ1(34) S-adenosylmethionine
                     ribosyltransferase-isomerase QueA"
                     /protein_id="HGU4547628.1"
                     /translation="MRVTDFSFELPESLIAHYPMPERSSCRLLSLDGPTGALTHGTFT
                     DLLDKLNPGDLLVFNNTRVIPARLFGRKASGGKIEVLVERMLDDKRILAHIRASKAPK
                     PGAELLLGDDESINATMTARHGALFEVEFNDERSVLDILNSIGHMPLPPYIDRPDEDA
                     DRELYQTVYSEKPGAVAAPTAGLHFDEPLLEKLRAKGVEMAFVTLHVGAGTFQPVRVD
                     TIEDHIMHSEYAEVPQDVVDAVLAAKARGNRVIAVGTTSVRSLESAAQAAKNDLIEPF
                     FDDTQIFIYPGFQYKVVDALVTNFHLPESTLIMLVSAFAGYQHTMNAYKAAVEEKYRF
                     FSYGDAMFITYNPQAINERVGE"
     gene            21865..22446
                     /gene="acpH"
                     /locus_tag="ACHOSC_RS08335"
                     /old_locus_tag="pgaptmp_001667"
     CDS             21865..22446
                     /gene="acpH"
                     /locus_tag="ACHOSC_RS08335"
                     /old_locus_tag="pgaptmp_001667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008770 - [acyl-carrier-protein]
                     phosphodiesterase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ACP phosphodiesterase"
                     /protein_id="HGU4547629.1"
                     /translation="MNFLAHLHLAHLAESSLSGNLLADFVRGNPEESFPPDVVAGIHM
                     HRRIDVLTDNLPEVREAREWFRSETRRVAPITLDVMWDHFLSRHWSQLSPDFPLQEFV
                     CYAREQVMTILPDSPPRFINLNNYLWSEQWLVRYRDMDFIQNVLNGMASRRPRLDALR
                     DSWYDLDAHYDALETRFWQFYPRMMAQASHKAL"
     gene            complement(22451..>23206)
                     /locus_tag="ACHOSC_RS08340"
                     /old_locus_tag="pgaptmp_001668"
     CDS             complement(22451..>23206)
                     /locus_tag="ACHOSC_RS08340"
                     /old_locus_tag="pgaptmp_001668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414937.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase family glycosyl hydrolase"
                     /protein_id="HGU4547630.1"
                     /translation="VAGITEAAKETQPEAYIVGEHFGDARQWLQADVEDAAMNYRGFT
                     FPLWGFLANTDISYDPQQIDAQTCMAWMDNYRAGLSHQQQLRMFNQLDSHDTARFKTL
                     LGRDIARLPLAVVWLFTWPGVPCIYYGDEVGLDGKNDPFCRKPFPWQVEKQDTALFAL
                     YQRMIALRKKSQALRHGGCQVLYAEDNVVVFVRVLNQQRVLVAINRGEACEVVLPASP
                     LLNAVQWQCKEGHGQLTDGILALPAISATVWMN"
BASE COUNT         5628 a         6207 c         5904 g         5467 t
ORIGIN      
        1 aatgcaaaaa aacatacctg acacacaaat tactctcctc tgtaatattt tctcattaac
       61 actctgttta cccaacatgc gctccgcact acactatgtg ggcatttttt taaggggtgg
      121 taatgaacga ttataaaatg acgccaggtg agaggcgcgc gacctggggt ttagggaccg
      181 tattctcgtt gcgcatgctg ggcatgttta tggttctgcc ggttctgacc acgtacggca
      241 tggctctgca aggtgccagc gaagcattaa tcggtattgc cattggtatt tatggtctga
      301 ctcaggccgt ttttcagatt ccgtttggcc tgctttccga ccgcattggt cgcaaaccat
      361 taattgtcgg tgggctggcg gtgtttgccg ccggtagcgt tatcgctgcg ctttccgact
      421 ccatctgggg aattattctg ggccgggcgc tacaaggctc tggagcaatt gccgccgccg
      481 ttatggcgct gctttccgat ctcacgcgcg aacaaaaccg caccaaagcg atggcgttta
      541 tcggcgtgag ctttggcatt acctttgcca ttgcgatggt gcttggcccg atcatcactc
      601 acaaacttgg gctgcacgcg ctgttctgga tgatcgctat tctggcaaca accggcattg
      661 cgttgaccat ttgggttgta cccaacagta gcactcacgt acttaatcgt gagtccggaa
      721 tggtgaaagg cagtttcagt aaagtgctgg cggaaccgcg gctgctaaaa ctcaactttg
      781 gcattatgtg tctgcacatg cttctgatgt ctacgtttgt tgccctgccc ggacagctag
      841 ctgatgcggg gttcccggcg gctgaacact ggaaggtcta tctggcgacg atgttaatcg
      901 cctttggctc ggtcgtgcct ttcattatct acgctgaagt taagcgcaaa atgaagcaag
      961 tctttgtctt ctgcgtcggg ttgatcgtgg ttgcggaaat tgtgttgtgg aacgcacaaa
     1021 cgcagttctg gcaactggtg gtcggcgtgc agcttttctt tgtggcgttt aatttgatgg
     1081 aagcccttct gccctcactt atcagtaaag agtcgccagc aggttacaaa ggtacggcga
     1141 tgggtgtcta ctccaccagc cagtttcttg gcgtggcgat tggcggttcg ctgggcggct
     1201 ggattgacgg catgtttgac ggtcaggggg tatttctcgc tggcgcaatg ctggccgcag
     1261 tgtggctggc agtcgccagt accatgaaag aaccgccgta tgtcagcagt ttgcgcattg
     1321 aaatcccggc ggacattgcc gcaaacgaag cgttaaaagt gcgtttgctg gaaactgaag
     1381 gcgtcaaaga agtgttgatt gcagaagaag aacattcagc ttatgtgaaa atcgacagca
     1441 aagtgacgaa tcgctttgag atagaacagg caattcgtca ggcataaaaa acgcggcgaa
     1501 ttgcctgctg cgctacgctt atcaggccta caaagtttct gaattacaaa cctttgtagg
     1561 ccgaatcagg cgttcatgcc gcatctgaca tgaacaaaac gcacatagtc gcgattaatc
     1621 gcggaagttt ttgaactgga acggctgacc gagatcgcca ccacgtacca tcgccatgac
     1681 agcctgcaaa tcatcacgag atttgcccgt tacgcggatt tcatcgccct gaatttgcgc
     1741 ctgcactttc agtttgctgt ctttgatcat cttgacgatt ttcttctggg tcgcgctctc
     1801 aatgccctgt ttcagtttcg cttccacaaa ccaggtttta ccgctatgaa cgatattctc
     1861 cggtacatcc agcgaactgc cttcaatgcc gcgcttcagc agcttggcac gcagaatatc
     1921 cagcaactga ttgacctgga aatcggactc gctcaacact ttgatggttt tgctggcgtc
     1981 gttcagctca aatgaggctt caacgttacg gaagtcaaaa cgggactcca cttcgcggct
     2041 cgcgttatcg accgcgttac gtgcttcctg aagatcaact tcagagacaa tatcgaaaga
     2101 tggcatcttt tcctctccct tcatttttga tgcgaagcat aatacccgca aagttaaata
     2161 accgacagtc ctgcaccagg acgtatccct taattgtgca tagtcgcaac agcatgcaca
     2221 attaagggat agccttattg aacacccgga gtggttgcgg gtgaggagga acaatgaaaa
     2281 ttaccgtatt gggatgcggt gccttagggc aattatggct tacagcactt tgcaaacagg
     2341 gtcatgaagt tcagggctgg ctgcgcgtac cgcaacctta ttgtagcgtg aatctggttg
     2401 agacagatgg ttcgatattt aacgaatcgc tgaccgccaa cgatcccgat tttcttgcca
     2461 ccagcgatct gctcctggtg acgctgaaag catggcaggt ttacgatgcc gtcaaaagcc
     2521 tcgcgtccac actgcctgta accacgccaa tactgttaat tcacaacggc atgggcacca
     2581 tcgaagagtt gcaaaacatt cagcagccat tactgatggg caccaccacc catgccgccc
     2641 gccgcgacgg caatgtcatt attcatgtgg caaacggaat cacgcatatt ggtccggcac
     2701 ggcaacagga tggcgattac agttatctgg cggatatttt gcaaaccgtg ttgcctgacg
     2761 ttgcctggca taacaatatt cgcgccgagc tgtggcgcaa gctggccgtc aactgtgtga
     2821 ttaatccact gaccgccatc tggaattgcc cgaatggtga attacgtcat catccgcaag
     2881 aaattatgca gatatgcgaa gaagtcgcgg cagtgatcga gcgcgaaggg catcatactt
     2941 cagcagaaga tttgcgtgat tacgtgatgc aggtgattga tgccacagcg gaaaatatct
     3001 cgtcgatgtt gcaggatatc cgcgcgctgc gccacaccga aatcgactat atcaatggtt
     3061 ttctcttacg ccgcgcccgc gcgcatggga ttgccgtacc ggaaaacacc cgcctgtttg
     3121 aaatggtaaa aagaaaggag agtgaatatg agcgcatcgg cactggtttg cctcgcccct
     3181 ggtagtgaag agactgaagc cgtcaccact atcgatctgc tggttcgcgg cggtatcaaa
     3241 gtcaccactg ccagcgtcgc cagcgatggc aacctggcca ttacctgctc gcgcggcgtg
     3301 aagctgctgg cggatgcgcc gctggtcgag gtggctgatg gcgaatatga cgtgatcgta
     3361 ctgcctggtg gcattaaagg cgcagagtgt tttcgcgata gtaccctgct ggttgaaacc
     3421 gttaaacagt tccaccgttc cgggcgtatc gtcgcggcta tttgcgccgc gccagccacc
     3481 gtgctggtgc cgcacgatat cttcccgatt ggtaatatga ccggcttccc gacgctgaaa
     3541 gacaaaattc ccgctgaaca atggcaggac aagcgcgtcg tctgggatgc acgggtaaaa
     3601 ttgctgacca gccaggggcc ggggacagct atcgactttg gtctgaaaat tatcgacctg
     3661 ctggttgggc gcgagaaagc ccatgaagtg gcatcacaac tggtgatggc ggcagggatt
     3721 tataattatt acgagtagtg tcggatgcgg taagcgtcgc atctgaccag atgcgacgtt
     3781 acaaccacaa attacgggcg atataccttc acattgttaa agccctgctc gcgcagatag
     3841 agcgcctgca gacggctcat caccccgcgc tcacaccaca gtagccaggt tctgttctgg
     3901 tcgagatcgc caaacttggt gctcagttta tagaacggca gagaaactac atcaatccct
     3961 tcgactttca gtggcttatc ttcctgttca tcgatagaac ggatatcgag gatcacgtcg
     4021 ttcgggccga agccattgac ggtttccact tccaccactt cctgctcggt ctgctgggcg
     4081 atttcgcgga tatcaacgtt attcgcttct tcaaccactt tatcgagaat gctgaagtcg
     4141 aatttctctt cttccgcttc aatcttcgac ttaaccgctt tcaccgtcgg gcttttggag
     4201 ataacaccgc aatattccgg catcgtgcga gcaaagtctt cggtgccaat ctggcgagcc
     4261 aggttgatga tgtgctcttt gtcgtaagaa atcagcggac gcaggatcag cgtatcggag
     4321 acgttatcaa tcaggcgcag gttggtcagc gtctgactgg acacctggcc gagcgcttcg
     4381 ccggtgacca gcgcctgtac gccgtaacgt tcagccactt tagacgcggc acgcaccatc
     4441 atacgtttga ggataacgcc catctgaccg tcgtcgattt tctcgagaat ttccccgacg
     4501 accggttcga aattaatagc gacaaaacgc acgcggtggg agctgccaaa acggttccac
     4561 agataatgcg ccacctgacg cacgccaatt tcatgcgccg cgccgccaag gttaaagaaa
     4621 cagtaatgca cgcggcagcc gcgacgcatc aacatataac tggaaacacc ggagtcgaaa
     4681 ccaccggaaa tgagcgacag cacatcttcc tgggtgccga tcgggaaacc gccaatacct
     4741 tcgtagcggc ctttaatcag caggagacga tcgtcttcca cttccagatg gacagtcact
     4801 tccggattgg tcagcttcac gcgcgcggac tcaatatgct gatttaaacc gccgccgacg
     4861 taacgttcca catcaatcga gctaaaatca tgtttgccac ggcgcttcac gcgtacgcag
     4921 aaggttttgc cttccagctg atcgcgatac tgaaccaacg ctttctcaaa aatatcgtgc
     4981 atgtcggtaa acggcacgtc ttcgacttcg agaatatggt ggatacccgg aatacgggtc
     5041 agggcgtcgc gaatagccag acgctggttt tcatcttttg cgcgaacttc gatgttatcc
     5101 cagtggcgga cgacagcgag cgtctcatca tagtgcttta aaacgttacg aatgttcccg
     5161 gtaaggattt ttataaagcg caagcgcaca gattggcttt tgatggtgat ttccgggaac
     5221 aatttaatga taaacttcat ggcggcaatg gttcgttggc aagccttaag cgacttgtat
     5281 agggaaaaat acagcagccc acacctgcgg ctgcatccag gcgcggaagt ataccactaa
     5341 catcgctttg ctgcgcacat caccttacca ttgcgcgtta tttgctattt gccctgagtc
     5401 cgttaccatg acggggcgaa aaatattgag agtcagacat tcattatgcc gaagaaaaat
     5461 gaggctcccg ccagctttga aaaggcgctg agcgagctgg aacagattgt aacccgtctg
     5521 gaaagtggcg acctgccgct ggaagaggcg ctgaacgagt tcgaacgcgg cgtgcagctg
     5581 gcacgtcagg ggcaggccaa attacaacaa gccgaacagc gcgtacaaat tctgctgtct
     5641 gacaatgaag acgcctctct aacccctttt acaccggaca atgagtaatg gactttccgc
     5701 agcaactcga agcctgcgtt aagcaggcca accaggcgct gagccgtttt atcgccccac
     5761 tgccctttca gaacactccc gtggtcgaaa ccatgcagta tggcgcatta ttaggtggta
     5821 agcgtctgcg acccttcctg gtttatgcca ccggtcatat gtttggcgtt agcacaaaca
     5881 cgctggacgc acccgctgcc gccgtagagt gtatccacgc ttactcatta attcatgatg
     5941 atttaccggc gatggatgat gacgatctgc gtcgcggttt gccgacctgc catgtgaagt
     6001 ttggcgaagc aaacgcgatt ctcgctggcg acgctttaca aacgctggcg ttctcgattc
     6061 taagcgatgc caatatgccg gaagtgtcgg atcgcgacag aatttcgatg atttctgaac
     6121 tggcgagcgc cagcggtatt gccggaatgt gcggtggtca ggcattagat ttagacgcgg
     6181 aaggcaaaca cgtacctctg gacgcgcttg agcgtattca tcgtcataaa accggcgcat
     6241 tgattcgcgc cgccgttcgc cttggtgcat taagcgccgg agataaaggg cgtcgtgctc
     6301 tgccagtact cgacaagtac gcagagagca tcggccttgc cttccaggtt caggatgaca
     6361 tcctggatgt ggtaggagat actgcaacgt tgggaaaacg ccagggtgcc gaccagcaac
     6421 ttggtaaaag tacctaccct gcacttctgg gtcttgagca agcccggaag aaagcccggg
     6481 atctgatcga cgatgcccgt cagtcgctga aacaactggc tgaacaatca ctcgatacct
     6541 cggcactgga agcgctagcg gactacatca tccagcgtaa taaataaaca ataagtatta
     6601 ataggcccct gatgagtttt gatattgcca aatacccgac cctggcactg gtcgactcca
     6661 cccaggagtt acgactgttg ccgaaagaga gtttaccgaa actctgcgac gaactgcgcc
     6721 gctatttact cgacagcgtg agccgttcca gcgggcactt cgcctccggg ctgggcacgg
     6781 tcgaactgac cgtggcgctg cactatgtct acaacacccc gtttgaccaa ttgatttggg
     6841 atgtggggca tcaggcttat ccgcataaaa ttttgaccgg acgccgcgac aaaatcggca
     6901 ccatccgtca gaaaggcggc ctgcacccgt tcccgtggcg cggcgaaagc gaatatgacg
     6961 tattaagcgt cgggcattca tcaacctcca tcagtgccgg aattggtatt gcggttgctg
     7021 ccgagaaaga aggcaaaaat cgccgtaccg tctgtgtaat cggcgatggc gcgattaccg
     7081 caggcatggc gtttgaagcg atgaatcacg cgggcgatat ccgtcctgat atgctggtgg
     7141 tcctcaacga caacgaaatg tcgatttccg aaaatgtcgg cgcgctcaac aaccatctgg
     7201 cgcagctgct ttccggtaag ctttactctt cactgcgcga aggcgggaaa aaagttttct
     7261 ctggcgttcc gccaattaaa gagctgctca aacgtaccga agaacatatt aaaggcatgg
     7321 tagtgcctgg cacgttgttt gaagagctgg gctttaacta catcggcccg gttgacggtc
     7381 acgatgtgct ggggcttatc accacgctga agaacatgcg cgacctgaaa ggcccgcagt
     7441 tcctgcatat catgaccaaa aaaggtcgtg gttatgaacc ggcagaaaaa gaccccatca
     7501 ctttccacgc cgtgcctaaa tttgatccct ccagcggttg tttgccgaaa agtagcggcg
     7561 gtttgccgag ctattcaaaa atctttggcg actggttgtg cgaaacggca gcgaaagaca
     7621 acaagctgat ggcgattact ccggcgatgc gtgaaggttc cggcatggtc gagttttcac
     7681 gtaaattccc ggatcgttac ttcgacgtgg caatcgccga gcaacacgcg gtgacctttg
     7741 ccgccggtct ggcgattggt gggtacaaac ccattgtcgc gatttactcc actttcctgc
     7801 aacgcgccta tgatcaggtg ctgcatgacg tggcgattca aaagcttccg gtcctgttcg
     7861 ccatcgaccg cgcgggcatt gttggtgctg acggtcaaac ccatcagggc gcttttgacc
     7921 tctcttacct gcgctgcata ccggaaatgg tcattatgac cccgagcgat gaaaacgaat
     7981 gtcgccagat gctctatacc ggctatcact ataacgacgg cccgtccgcg gtgcgctacc
     8041 cgcgcggtaa cgcggttggc atggaactga cgccgctgga aaaactgcca attggcaaag
     8101 gcattgtgaa gcgtcgtggc gagaaactgg cgatccttaa ctttggtacg ctgatgccag
     8161 acgcggcgaa agtcgctgaa tcgctgaacg ctacgctggt cgatatgcgt tttgtgaaac
     8221 cgcttgatga agcgttaatt ctggaaatgg ccgccagcca tgaagcgctg gtcaccgtag
     8281 aagaaaacgc cattatgggc ggcgcaggca gcggcgtgaa cgaagtgcta atggcccatc
     8341 gtaaaccagt acccgtgctg aacattggcc tgcctgactt ctttattcca caaggaactc
     8401 aggaagaaat gcgcgccgaa ctcggcctcg atgccgccgg tatggaagcc aaaatcaagg
     8461 cctggctggc ataatcccta ctccactcct gctatgctta agaaattatt catagactct
     8521 aaataattcg agttgcagga aggcggcaaa cgagtgaagc cccaggagct tacataagta
     8581 agtgactggg gtgaacgaat gcagccgcag cacatgcaac ttgaagtatg acgagtatag
     8641 cgggagtggc agcatgcaat acaacccctt aggaaaaacc gaccttcgcg tttcccgact
     8701 ttgcctcggc tgtatgacct ttggcgagcc agatcgcggt aatcacgcat ggacactgcc
     8761 ggaagagagc agccgtccca tcattaaacg cgcgctggaa ggcggcataa atttctttga
     8821 taccgccaat agctattccg atggcagcag cgaagagatc gttggtcgcg cactgcggga
     8881 tttcgcccgt cgtgaagacg tggtcgttgc gacgaaagtg ttccatcgcg ttggtgattt
     8941 accggaagga ttatcccgtg cacaaatttt gcgctctatc gacgacagcc tgcgccgtct
     9001 cggcatggat tatgtcgata tcctgcaaat tcatcgctgg gattacaaca cgccgatcga
     9061 agagacgctg gaagccctga acgacgtggt aaaagccggg aaagcgcgtt atatcggcgc
     9121 gtcatccatg cacgcttcgc agtttgctca ggccctgaag ctacaaaaac agcacggctg
     9181 ggcgcagttt gtcagtatgc aggatcacta caatctgatt tatcgcgaag aagagagcga
     9241 gatgctgcca ctgtgttatc aggagggcgt ggcggtgatt ccgtggagcc cgctggcgcg
     9301 ggggcgactg acgcgtccgt ggggagaaac taccgcacga ctggtgtctg atgaggtggg
     9361 gaaaaatctc tataaagaaa gcgatgaaaa tgacgcgcag atcgcagagc ggttaacagg
     9421 cgtcagcgaa gaactcggtg caacacgagc acaagttgcg ctggcctggt tgttgagtaa
     9481 accgggcatt gccgcaccga ttatcggaac ttcgcgcgaa gaacagcttg atgagctatt
     9541 gaacgcggtg gatatcactt tgaagccgga acagattgcc gaactggaaa cgccgtataa
     9601 accgcatgcg gtcgtaggat ttaaataaga gtccaggcct gatgagacgt gacaagcgtc
     9661 acatcaggca tcggtgcaca actacgacag aatacccagc ggccagtgat gaccgataaa
     9721 gtacaggatg cctgcggaaa tcaccccggc gacaatatcg tcgatcatga tccccatgcc
     9781 gccatgtaca ttgcgatcaa accagcggat cggccacggc ttccacatat ccagaatacg
     9841 gaaaatcaca aacccggcgg caacccactg ccagtcattg gtcggcagcg ccatgagcgt
     9901 gatccacata ccaataaatt cgtcccagac aatgctgccg tgatcgtgca cgcccatatc
     9961 tttcgccgtt tgatgacaaa gatagacgcc gatacagatc cccaacatca ccaccagcga
    10021 gtagagctgc cagggtaaaa aggtcatcag ataccagaac ggaatcgctg ccagcgatcc
    10081 catcgtgcca ggaacgattg ggcttaatcc acttccgaat ccgacagcaa gcagatgcca
    10141 cggattactc atcttcaggc gacttttcgc gacatcttta tggcgtggca aaatggtcat
    10201 atcctttcca gtcaaacgtg acaggttcgc cgtcacgaat aaaacaaagc ccttcgatat
    10261 cggcggtcat ttgcccgata caggtaaacg gtacgcccag gtgtccgaga gccacatcca
    10321 gcgcgccacg gttcagttcc ggcacagtga aacacaactc gtaatcttca ccgccagaga
    10381 gcgcccagcg cagcgcctgt tccggttcaa catggcgaga aagcgcatca gaaaacggca
    10441 gcaatgccag gtcaatacgt gcgccgcagt cgctggcttt cacgatatgc ccgagatcgg
    10501 aaatcagacc gtcagagaga tcgatagctg aatttgccag atcgcgcagt gcctgcccct
    10561 gtaaaatacg cggcgatgga cggagatgac gtttgatcaa gtagtcagca tctttagcat
    10621 cggcaacctg caaacggttt tgcaaaatcg ccagcccggc ggcgctatcg cccggtgtac
    10681 cggtcacata gatccagtca cccggtttcg ccccagagcg cgttaaggct cgtcccatcg
    10741 gaacaaagcc gtggataccc aacgtcattg ataatggccc acgcgtggta tcgccgccaa
    10801 tgagttgcat atcgtaataa ttgagaagat caaacaaact gtcgctgaag gactcaagcc
    10861 acgcttcgtc tacgtccggt aaggttaatg ccagcgtcag ccaggccgga tcggccccca
    10921 tcgctgccag atcgcttagg ttcactgcca gtgctttata cgccagatca gcaggatcga
    10981 tatcatggag gaagtggttg cccgccacca gcgtatcagt gctgatcgcc agggtctgtt
    11041 tctcggggat attgagaaga gcgcaatcgt cgccgatgcc cagttcgaca tcaagacgag
    11101 aacttcttac acggtcaaaa taacgggcaa tcagggagaa ctcgccacat gccatacgtt
    11161 atgcctcagc aggtaaaaaa gaaaaggccg gcgatcgcgg aacggtcttc cgtgaatcta
    11221 ccggcctgga tatcactttt tgttagggcg aatcacaggt gctgctttat cgagtacgcc
    11281 gttgacgaac ttatggctgt cttctgcgcc gaacgatttc gccagttcga tcgcttcgtt
    11341 aatggccact ttgtatggca catcgctacg tttagacagc tcgtacagcg caatgcgcag
    11401 tactgctttt tctacctgtc ccagttcttc cagcaggcgg gacaggtatg gcttcatcag
    11461 tccgtcgagg tatgcggtgt tagtcgccac cccggccagc agctcacgga agtacaggac
    11521 gtcaacgtct tttacatcct gttcagccag gaactggtat tcaacatcag cgatgtcgtt
    11581 ctgggacaac tgccaggagt agagcgcctg gacggcacac tcacgagcgc ggcgacgagc
    11641 agcaggtttc acggatttcc ccttactaat ttcaggcctt gatggctttc aatacattaa
    11701 tcatttcaag cgcggtcagt gcagcttctg cacctttgtt gccagctttg gtgccagcac
    11761 gttcgatcgc ttgttcaatg ctttcagtgg tcagaacccc aaaagcaacc ggaatttcgc
    11821 tgtcctgggc aacatgcgcc aggccgttgc ttgcaccacc agcgacatat tcaaagtggg
    11881 cagtgccacc acgaataacc gtacccagcg caatcaccgc gtcgtattta ccggttttag
    11941 ccagtgcacc cgccgccagc ggcagctcat aggcaccagg cacccaaaca acggtaatgt
    12001 tttcatcttt tacctgaccg atacgtttca gtgcgtcaat tgcaccttcc agcaggctgt
    12061 cattgataaa gttgttgaaa cgcgcgatgg tgatggcgac gcgagcgtcc ggggtagcaa
    12121 cgttagcttc aataatgttc attttcttcc ttcgggttcg agtatggccc cgcagggggg
    12181 cggattttag cataatattt cgtgcgctgc ttccctttcg agccgggaga tcatgcaccc
    12241 actaaatgca ggcaaacatc cgggcctaca tgacgtatct ctttgaattt aaattggggg
    12301 gcgtcggcta atttctcaag ccctggcagc gtgcataatc cgcgggcgtc gctgcctaat
    12361 agtttaggtg cgatatagac aatcagctca tcgactaaac ccgcctgcag caatgcgcca
    12421 gcgagcgttg gccccgcttc cacccagata ctgttaattt gctgtttacc cagttgcatc
    12481 atcagtacaa ccagatccag atgaccttta tgctctggaa tcagcaaggt acgcaccgtt
    12541 tccggccact cactagaatc ttcctgcgta cgcgcgaacc aggtttcgcc gggctgctgc
    12601 acaatgcgat gttccggcgt cacgcgattt tggctatcaa tcacaatacg tactggctgg
    12661 cggagatttt gttgcgggta gagcgcctga gtttgttcat ccagttcaga ccaacgcact
    12721 gttaaggctg gatcatccgc cagcaccgtg gcgctgctag ttaaaatggc atgactttgt
    12781 gcgcgctgtc gttgtacgtc gcgccgcgcc tgaggcgaag tgatccactg gctttcgccg
    12841 ctcgccatcg ccgtgcggcc atcaagcgat gcgccaagtt ttaactgaat ataaggaaag
    12901 ccggtgcgca tccgcttgag aaagccttta ttcaattgct cggcttcact catcatcagc
    12961 ccgtggctga cgtcaatgcc agcctgttgc agacggtaaa gtccacgccc agcgacctga
    13021 gggttaggat cttgcatcga ggcaaccacg cgcgctacgc cagcggcgat gagtgcgtca
    13081 cagcacggtg gcgtacgacc atgatggcta cagggttcga gtgtgacata ggcggtcgca
    13141 cctttggctt tttcacccgc catacgcaac gcgtgtactt cggcatgtgg ttcacccgca
    13201 cgttggtggt aaccttcacc gacaatttcg ccatctttga caatgacgca cccgacattc
    13261 gggttgggat gcgtggtaaa acgtcctcgt tgcgccagct ttagcgctcg cgccatgtaa
    13321 tactcgtcct gcacggctta gtcctccagg cgcgcgatct cttcgccaaa ttctttgata
    13381 tcttcgaaac tgcggtagac agaggcaaaa cggatatagg cgactttatc gagctttttc
    13441 aattgctcca tcaccagatt gccaatcatc ttgctcggca cttcgcgctc accggtggcg
    13501 cgcagctgcg atttaatatg attgattgcc atttcgacgt catcggaact caccggacgt
    13561 ttttccagcg cccgcagcat tccgctacgc aatttctctt cattaaacgg ttcacgcacg
    13621 tcgttgcttt ttacaacacg cggcataacc agctccgcca cttcaaaggt ggtgaaacgt
    13681 tcattacaca ccagacactg ccgacggcgg cgtacggatg aaccctcgcc cacgagacga
    13741 gagtcaatta ccttagtgtc cacggcgaaa cagaatgggc aatgcatacg gcgtcctgac
    13801 caggtggtta acagaaatct attttaccct gaactgacga tacaacaaag gcgcaacgct
    13861 tttgagataa aggatcgatg ctttcgctgc ttttaccctc taccattagg ctaattgcga
    13921 cgagaaaggg acgacctatg aatactaacg tttttcgact gctcctgctg ggaagcctgt
    13981 tcagccttag cgcctgtgtg cagcagagcg aagtgcgaca gatgaaacac agcgtcagca
    14041 cgctgaacca agagatgacg caactcaata aagaaacggt caaaatcact cagcaaaaca
    14101 ggctgaatgc aaaatccagc agtggggttt accttctgcc aggagcgaaa acaccggcaa
    14161 gactggaaag ccagatcggt actttacgta tgtcgctggt gaatattacg cctgatgcag
    14221 atggcaccac cctgacgcta cgtattcagg gcgagtcgaa tgatcccttg cccgcattca
    14281 gcggaactgt tgaatatggg caaattcagg gaacaataga caactttcag gaaatcaatg
    14341 tgcagaatca attgattaat gcccctgcca gcgtcctcgc ccccagcgat gttgatattc
    14401 cgttacagtt aaagggtatt tctgtagaac agttagactt cgtacgtatc cacgatattc
    14461 aacctgttat gcagtaaacg tatttcgggg cgattttgtg cgtctcgcaa catctttccc
    14521 cgtcattttg ttactccgct tacatcaccc tgattgatag taaaagtttg caacaagggt
    14581 gaaagtcagt acaatccctg cccgaatgtg tgtaaacgtg aacgcaatcg attacgtaaa
    14641 tgatagaact gtgaaacgaa acatattttt gtgagcaatg atttttataa taggctcctc
    14701 tgtatacgaa atatttagaa acgcaatttg cgcctttttc actcccgcaa gggattttca
    14761 aacagtggca tacatatgaa aaaaacatta ctggcagccg gtgcggtact ggcgctctct
    14821 tcgtctttta ctgtcaacgc agctgaaaac gacaaaccgc agtatctttc cgactggtgg
    14881 caccagagcg ttaacgttgt cggaagctat cacacccgtt tcggaccgca gatccgcaac
    14941 gatacctacc ttgagtacga agcattcgct aaaaaagact ggttcgactt ctatggttat
    15001 gcggatgcgc cggtattctt cggcggtaac tccgatgcta aaggtatctg gaaccacggt
    15061 tctccgctgt ttatggaaat cgaaccacgt ttctccatcg acaagctgac caatactgac
    15121 cttagcttcg gtccgttcaa agagtggtac ttcgcgaaca actacattta cgacatgggt
    15181 cgtaataaag atggtcgcca gagcacctgg tacatgggtc tgggtaccga catcgatact
    15241 ggtctgccga tgagcctgtc catgaacgtc tatgcgaaat accagtggca gaactatggc
    15301 gcagcgaacg aaaacgagtg ggacggttac cgtttcaaaa ttaaatactt tgtgccgatt
    15361 accgatctgt ggggcggtca gctgagctac atcggcttca ccaacttcga ctggggttcc
    15421 gatttagggg atgacagcgg taacgcaatc aacggtatta agacccgtac taataactct
    15481 atcgcttcca gccatattct ggctctgaac tacgatcact ggcactactc tgtcgtagct
    15541 cgttactggc acgacggtgg tcagtggaac gacgatgcag aactgaactt cggcaacttc
    15601 aacgttcgct ctaccggctg gggtggttac ctggttgtag gttacaactt ctgattatga
    15661 aaatgccggg atttattccc ggcatttcag attgttaaca acgtgcgctt tgttcatgcc
    15721 ggatgcggcg taaacgcttt gtccgaccca caaaagcacg taaattcaat atattgctga
    15781 aataacgtag tcctgataag cgtagcgcat caggcaattt tacgttttaa tcacttcttc
    15841 ttgttcatca tcgctttcag gtcagcgaat gggttgtact tcgcttcacc gacatctttc
    15901 tgcgcatctt cccctgcgat aacggtcgta ccatactgat ccgcttcggt atatttcgaa
    15961 tgctcatgat cgtggcaata gagacaaagc aattcccagt tactgccatc ttccgggtta
    16021 ttggtatggt cgtgatcaat gtggtgaaca gtaagttcac gcaggttgga ataaacaaac
    16081 tcgcgggaac agcgaccgca gacccacgga tatattttta atgctttttc gcgatagccg
    16141 ctttctaacc gcgcatagtt ttttgggatg atagccatga agagttacct ggatgaaatg
    16201 tagggttacg cgcagtttcc caagatgcgg ggattttcgc aatctccata catcaaaaaa
    16261 aatcccgatc ttctgaccgg gattttcaac atcaacggga acttgattac ggcagaattg
    16321 acggctgatc cgccccttct ttttccactt tctgctgcaa catgtgttcg cgcttcatac
    16381 ccagtttcag agccaacgca gatgccacat agatggaaga tgcagtaccg atggaaacac
    16441 cgataagcat ggtcagcgag aagccttcca gtaccggacc accgaagagg tacagcatca
    16501 ggataaccat caaggtagta ccggatgtga tcaaggtacg gtgcagcgtc tgggtcaagg
    16561 acacgttaaa gatttcgtaa ggcgtaccgc gacggatctt gcggaagttt tcacgaatac
    16621 ggtccgatac cacgatactg tcgttaagcg agtaaccgat aaccgacatc aacgatgcca
    16681 caatggtcag gtcaatctcg atatggaata acgacaaaat acccagcgta atgataacgt
    16741 cgtgcgccag cgcaataacc acccctgccg ccagtcgcca ctcaaagcga aaacctacgt
    16801 acacaaggat agacagcagc gccgccatca acgccatcgc accggtttgc gcaaggtctg
    16861 cccccacgct cggaccgacg aactcaatac gcttcactgc tgcattctga ttggtggatt
    16921 cgttaatcac cttcagaacc tggctgccca gcacctgacc gccggtttcg ccttcagcag
    16981 gcggcatacg gaccatgatg tcgtggctgc taccaaagtt ttgcagcatt ggctcttcaa
    17041 aaccggcttt ttgcaatgca tcacgcatta cgtcaatttc agccggtttt tcgagcgtaa
    17101 tttcgataac cgtaccaccg gtgaaatcca gcccccagtt aaagccgcgc acgcccataa
    17161 taacgatagc ggcgattaac agcagaccag agatgccgaa agcccagtag tcccagcgca
    17221 taaagtcata gactttacgg ccgtggttta gttgttcaac agtatattcc tgtgccacat
    17281 cgcactcctc agattgacag cttcttgacg cgcttgccgc catatagcag gtttacgatg
    17341 gcacgcgtac cgacaatcgc ggtaaacatc gacgtcgcca caccgatacc ggtagtaatc
    17401 gcgaaccctt taattgcccc ggtacccact gcgtacagga tgatgacttt aatcagcgtg
    17461 gtgatgttcg catcgaagat agaactgaat gcgccacgat aaccttcatc aattgcctgt
    17521 tgaacagtac gtccgttgct caactcttct ttaatacgtt cgttgatcag tacgttcgca
    17581 tcgaccgcca ccgcaagggt taagacgata cccgcgatac ctggcatact cagcgttgcg
    17641 cctggcaaca gcgacataat gccgacgatt aagatcaagt tggcaatcag agcactggtc
    17701 gcaatcagac caaacttctt atagaagatg atcatgaaca gaatagaaac cagcagaccg
    17761 gcaaggcaag cctccagccc ctgttcaatg ttctgcatac ccagggttgg gccaatggta
    17821 cgttcttcaa caatctgaat cggcgcgatc aacgcaccgg cacgcagcag cagtgacagc
    17881 tgacgggctt cgttcgggtt gttgatgccg gtgatacgga agctgttacc cagacgagac
    17941 tggatgttgg cgatgttaat cacctcttcc tgtttcacca gaaccgcacg accatttgca
    18001 tctttcttac cgctgtcttt gtactccaca aacagggttg ccatcggttt gccgatgttg
    18061 tccttagtga agttagacat gatgttacca ccagcgctat cgagcgagat gttaacctgc
    18121 ggttggttgt attcgtcctg gctggaagtg gagtcggtga tatggtcacc ggtcagaatt
    18181 acgcgtttgt acagcacaac tggctgacct tcgcgggtct gtttcacttc agagtcgccc
    18241 ggtacgcgac cggatgccgc agcggcctgg tcaacgttgg tgtttaccag acggaattcc
    18301 agcgttgccg tcgcacccag aatctctttc gcacgcgcag tgtcctgaat acctggcagt
    18361 tcaacaacga tacggtcagc accctgacgc tgaaccaccg gctccgccac gccaagttgg
    18421 tttacacggt tacgcaggat attgatgttc tgctgcaccg catattcacg cgcttcactc
    18481 agacgagcat cgctcattac cgcacgcagc tggttgctgc cctggctact aatcaccagg
    18541 tccggatggc gcttgctcag atacgcaatg gcttcatcac gagctttagc atcgcggaaa
    18601 gtaatgctca ggccgtagtt gttttcttta cgaacggtgg tatacgggat gcccttttca
    18661 cgcagatcgc tgcgcaggct atcgatattt tgttcctgga gtttgccaag cgcggtgtcc
    18721 atatccactt ccatcaggaa gtgaacgccg ccacgcaggt caaggccgag cttcatcggc
    18781 tcagcgtgaa tagctgccag ccagcgcggc gttgccgggg caaggttaag cgccacgacg
    18841 tatttgtcac ccataacgcc cattaatgct tcacgagcgc gcagctgggt gtcagtggag
    18901 tcgaagcgcg caagaatagc gccctcttcc agtgccacag acttagcagt tattttttct
    18961 tcttgtaacg ttttctggac ctggatcagc gtttgctcac tggcggcgac tccgcgcgca
    19021 ccagtgatct gaacagccgg atcctcacca aacaggttgg gaagcgcata cagcagaccg
    19081 atgacaatca ccacgatcag catgacgtac ttccacaaag gataacggtt taacacggca
    19141 attcccttag ggaaaaattt taattacagc gccttcatgg tgcctttcgg caggacggca
    19201 gctacgaagt cacgtttaat aactacttca gtggtgtcat tcagcgcgat agcaatgtag
    19261 ccgttttccg ctactttggt tacgcgacca accaggccac cgttcgtcag aacttcatca
    19321 cctttggcaa tggagtccat cagctttttg tgttctttgg tgcgcttctg ctgtggacgc
    19381 aggatcatga aatagaaaat cagaccgaat accaccagca tcaaaatcaa agacatcggg
    19441 ctaccttgcg ccggtgcacc cgttgccgct accgcatcag aaataaaaaa gctcattaaa
    19501 tttccctcat tattaatatt aatcaacgtt caaaggtggt acttctcgcc cctgacgctg
    19561 gtaaaaatca gttacgaagc tctctaattt accctcttca atagccttgc gtaaacccgc
    19621 catcaaacgc tggtagtaac gaaggttatg aatggtgttg agtcgcgcgc ctaatatttc
    19681 gttgcaacgg tcaagatgat gcaagtaagc gcgtgaataa ttgcgacagg tgtagcaatc
    19741 acactcagga tcgagtgggc cagtatcgct cttatacttc gcattgcgga ttttcaccac
    19801 gccatcggtc acgaacaaat gaccatttcg ggcgttgcgg gttggcatta cgcagtcaaa
    19861 catatcgata ccacgacgta cgccttcaac caggtcttct ggtttaccaa cgcccatcag
    19921 gtaacgcggt ttgtctgccg gaatttgcgg gcatacatgc tccagaatgc ggtgcatatc
    19981 tgctttcggc tcacccacag ccagaccgcc gacagcgtag ccatcaaaac cgatatctac
    20041 cagaccttta acagaaatat cacgtaaatc ttcgtaaacg ctgccctgaa tgataccaaa
    20101 cagcgcattt ttgtttccga gactgtcaaa acgctcacgg ctacgcttcg cccaacgcag
    20161 agacatctcc atggagcgtt ttgcgtaatc ccagtcagca ggatacggcg tacactcatc
    20221 aaagatcatg acgatatccg aaccaagatc gtactgaatc tccattgatt tttcaggatc
    20281 gaggaaaatc ggatcgccgt tgatcgggtt acggaagtgc acgccctgtt cggtgatttt
    20341 acgaatatcg ccaaggctga agacctggaa gccgccggag tcggtgagga tcggcccctt
    20401 ccactgcata aaatcgtgca gatcgccgtg cagtttcatg atttcctggc ccgggcgcag
    20461 ccacaggtgg aaggtgttgc cgaggataat ttgcgcgcca gtggcttcaa cttcttccgg
    20521 cgtcatccct tttacggtgc cgtaggtgcc aacaggcata aaacaaggcg tttccactac
    20581 gccacaatca aagaccaggc ggccacggcg tgcgcgaccg tcggtggtgt ccagttcaaa
    20641 tttcattttt tctccactac gtcagaaaaa cagtccaacg ttttaaacca gcgccgcgga
    20701 attactcccc gacgcgctca ttaattgcct gcggattgta cgtgataaac atcgcatcac
    20761 cgtaactaaa aaagcgatat ttctcttcta ccgctgcttt ataggcgttc atggtgtgtt
    20821 gataaccggc aaaggccgaa accagcataa tcagcgtcga ttccggcagg tggaagtttg
    20881 tcaccagcgc atcgaccact ttgtactgga agcccggata gataaagatt tgggtatcgt
    20941 caaagaacgg ttcgataaga tcgtttttcg ccgcctgagc cgcgctttcc agcgaacgta
    21001 ctgaggtggt gccaaccgca atcacccggt taccgcgcgc tttcgccgcc agtaccgcgt
    21061 ctaccacatc ctgcggcact tcagcgtatt ccgagtgcat gatgtgatct tcaatggtgt
    21121 cgacgcgcac cggctggaag gtgcccgcac caacgtgcaa cgtcacaaac gccatctcca
    21181 cgcctttggc gcgtaatttt tccagcaaag gttcgtcaaa atgcagacct gcggtcgggg
    21241 ctgcaaccgc gcccggtttt tcgctataaa cggtttgata aagttcgcgg tcagcgtctt
    21301 cgtccggacg gtcgatatac ggcggcagcg gcatatggcc gatgctgttg agaatatcca
    21361 gcaccgagcg ttcatcatta aattcgactt caaacagtgc gccgtggcgc gcggtcattg
    21421 ttgcgttaat actttcgtca tcgcccagca gcagttctgc gccaggtttt ggcgctttcg
    21481 aggcgcgaat atgcgcaaga atgcgtttgt cgtcgagcat ccgttcaacc agcacttcaa
    21541 tcttgccgcc gctggcttta cgcccaaaca ggcgcgccgg gatcacgcgg gtattattaa
    21601 aaaccagaag atcgccgggg ttgagcttat caagtaaatc ggtgaaagta ccgtgcgtca
    21661 gcgcgcccgt cggcccgtcc agcgacagta aacgacagct actgcgttca ggcatgggat
    21721 agtgggcaat cagggattcg ggcaattcaa aggagaaatc ggtaacgcgc atgacactga
    21781 ctcagactaa aataagaggc gggtagtcta gtgccggggc gctctccctg caacctttac
    21841 ccccttttgg atagaatata atcgatgaat tttttagctc acctgcattt agcccatctc
    21901 gcggaaagct cgctttccgg caatttactg gctgatttcg tacgcggaaa tcctgaagaa
    21961 agttttccgc ccgacgtcgt ggctggcatt catatgcatc gacgtatcga cgtattaacc
    22021 gacaatctgc cggaagtccg cgaagcacgg gaatggtttc gtagtgaaac tcgccgcgtt
    22081 gcgcctatta cgctggatgt catgtgggat cactttcttt cccgccactg gtcgcagctg
    22141 tcgccggact ttccgctaca ggaatttgtc tgttatgccc gcgagcaagt gatgacgatt
    22201 ttgccggact caccgccacg ttttatcaat ctgaacaatt acttgtggtc agagcagtgg
    22261 ctggtgcgct atcgcgatat ggatttcatc cagaacgtgt taaacggcat ggcaagccgc
    22321 cgcccacgtc tggatgccct gcgtgactcc tggtacgatt tagacgctca ttatgacgcc
    22381 ctcgaaaccc gcttctggca gttttatccg cggatgatgg cgcaggcgtc acacaaggcg
    22441 ttataaaacg ttagttcatc cataccgtag ccgaaatggc aggcaaagcc agaatcccgt
    22501 cagtcagttg cccatgccct tctttgcatt gccattgcac ggcattgagc aacggtgacg
    22561 cgggtagcac cacttcacaa gcttcgccac ggttgattgc caccagtaca cgctgctgat
    22621 tcagcacgcg gacaaatacc accacgttat cttccgcata cagcacctga cagccgccat
    22681 gacgtagcgc ctgacttttc ttacgcagcg caatcattcg ctggtacagc gcgaataacg
    22741 ccgtatcctg cttttccacc tgccagggga acggtttacg gcaaaacgga tcgtttttgc
    22801 catccagtcc tacttcatca ccgtaataaa tgcacggtac accaggccag gtgaacagcc
    22861 agaccaccgc cagcggcagg cgcgcaatat cccgaccgag cagcgtttta aatcgcgcag
    22921 tatcgtggct gtcgagctga ttaaacatac gtaattgttg ttgatgagaa agccctgcgc
    22981 ggtaattatc catccaggcc atacaggttt gggcatcaat ttgctgcgga tcgtaagaga
    23041 tatcggtatt ggcaagaaat ccccacaacg ggaatgtgaa gccacgatag ttcatggcgg
    23101 catcttccac atcggcctgt aaccattgcc gtgcatcgcc aaaatgttcg ccgacaatat
    23161 acgcttccgg ctgggtttct ttcgccgctt cggtgatccc agcaac
//
LOCUS       DAZKHF020000053        23060 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2201S31241
            SAMN43267800-rid25106413.denovo.053, whole genome shotgun sequence.
ACCESSION   DAZKHF020000053 DAZKHF020000000
VERSION     DAZKHF020000053.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267800
            Sequence Read Archive: SRR30307263
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 23060)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23060)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 23060)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350581.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350581.2
            Genome Coverage       :: 151x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:21:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,652
            CDSs (total)                      :: 5,557
            Genes (coding)                    :: 5,320
            CDSs (with protein)               :: 5,320
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 237
            CDSs (without protein)            :: 237
            Pseudo Genes (ambiguous residues) :: 0 of 237
            Pseudo Genes (frameshifted)       :: 102 of 237
            Pseudo Genes (incomplete)         :: 130 of 237
            Pseudo Genes (internal stop)      :: 52 of 237
            Pseudo Genes (multiple problems)  :: 41 of 237
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23060
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267800-rid25106413.denovo.053"
                     /serotype="O 26:H11"
                     /isolate="2201S31241"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-01"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            129..944
                     /gene="yahL"
                     /locus_tag="ACHOSC_RS08345"
                     /old_locus_tag="pgaptmp_001669"
     CDS             129..944
                     /gene="yahL"
                     /locus_tag="ACHOSC_RS08345"
                     /old_locus_tag="pgaptmp_001669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YahL"
                     /protein_id="HGU4547631.1"
                     /translation="MISLKAPHNNLMPYTQQSILNTVKNNQLPEDIKSSLVSCVDIFK
                     VLIKQYYDYPYDCRDDLVDDDKLIHLMAAVRDCEWSDDNALTINVQFNDFPGFYDWMD
                     YPDHPVKFVFRILENQKGTVWVYDQDDAFLDIKANVQAGRFTGLKKLVQFIDSVRTDC
                     KCILLEHHMPLLRIFPKGKECMRVEKWLREMSSIPETDAPIKQALAHGLLLHLKNIHP
                     VFPESLVMLLLSVLDVKTYRDDARLNEWISNRVQELGDRYYPVNKHVKIRYTL"
     gene            1356..1601
                     /gene="yahM"
                     /locus_tag="ACHOSC_RS08350"
                     /old_locus_tag="pgaptmp_001670"
     CDS             1356..1601
                     /gene="yahM"
                     /locus_tag="ACHOSC_RS08350"
                     /old_locus_tag="pgaptmp_001670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414861.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YahM"
                     /protein_id="HGU4547632.1"
                     /translation="MAVQLFKTLLNQIPLLSSLQSGTLPLFGYSGRVRPMKKAHTGES
                     GLKWEAKDSSKLIGNKDHALRGQSSPMVGIRQIRLIT"
     gene            complement(1618..2289)
                     /gene="yahN"
                     /locus_tag="ACHOSC_RS08355"
                     /old_locus_tag="pgaptmp_001671"
     CDS             complement(1618..2289)
                     /gene="yahN"
                     /locus_tag="ACHOSC_RS08355"
                     /old_locus_tag="pgaptmp_001671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysE family transporter"
                     /protein_id="HGU4547633.1"
                     /translation="MMKLLHLFMDEITMDPLHAVYLTVGLFVITFFNPGANLFVVVQT
                     SLASGRRAGVLTGLGVALGDAFYSGLGLFGLATLITQCEEIFSLIRIVGGAYLLWFAW
                     CSMRRQSTPQMSTLQQPISAPWYVFFRRGLITDLSNPQTVLFFISIFSVTLNAETPTW
                     ARLMAWAGIVLASIIWRVFLSQAFSLPAVRRAYGRMQRVASRVIGAIIGVFALRLIYE
                     GVTQR"
     gene            2436..2711
                     /gene="yahO"
                     /locus_tag="ACHOSC_RS08360"
                     /old_locus_tag="pgaptmp_001672"
     CDS             2436..2711
                     /gene="yahO"
                     /locus_tag="ACHOSC_RS08360"
                     /old_locus_tag="pgaptmp_001672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1471 family periplasmic protein YahO"
                     /protein_id="HGU4547634.1"
                     /translation="MKIISKMLVGALALAVTNVYAAELMTKAEFEKVESQYEKIGDIS
                     TSNEMSTADAKEDLIKKADEKGADVLVLTSGQTDNKIHGTANIYKKK"
     gene            complement(2809..4395)
                     /gene="prpR"
                     /locus_tag="ACHOSC_RS08365"
                     /old_locus_tag="pgaptmp_001673"
     CDS             complement(2809..4395)
                     /gene="prpR"
                     /locus_tag="ACHOSC_RS08365"
                     /old_locus_tag="pgaptmp_001673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="propionate catabolism operon regulatory protein
                     PrpR"
                     /protein_id="HGU4547635.1"
                     /translation="MAHPPRLNDDKPVIWTVSVTRLFELFRDISLEFDHLANITPIQL
                     GFEKAVTYIHKKLANERCDAIIAAGSNGAYLKSRLSVPVILIKPSGYDVLQALAKAGK
                     LTSSIGVVTYQETIPALVAFQKTFNLRLDQRSYITEEDARGQINELKANGTEAVVGAG
                     LITDLAEEAGMTGIFIYSAATVRQAFSDALDMTRMSLRHNTHDATRNALRTRYVLGDM
                     LGQSPQMEQVRQTILLYARSSAAVLIEGETGTGKELAAQAIHREYFARHDARQGKKSH
                     PFVAVNCGAIAESLLEAELFGYEEGAFTGSRRGGRAGLFEIAHGGTLFLDEIGEMPLP
                     LQTRLLRVLEEKEVTRVGGHQPVPVDVRVISATHCKLEEDMRQGQFRRDLFYRLSILR
                     LQLPPLRERVADILPLAESFLKVSLAALSAPFSAALRLGLQASETVLVHYDWPGNIRE
                     LRNMMERLALFLSVEPTPDLTPQFLQLLLPELARESAKTPAPRLLTPQQALEKFNGDK
                     TAAANYLGISRTTFWRRLKS"
     gene            4634..5524
                     /gene="prpB"
                     /locus_tag="ACHOSC_RS08370"
                     /old_locus_tag="pgaptmp_001674"
     CDS             4634..5524
                     /gene="prpB"
                     /locus_tag="ACHOSC_RS08370"
                     /old_locus_tag="pgaptmp_001674"
                     /EC_number="4.1.3.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046421 - methylisocitrate lyase activity
                     [Evidence IEA];
                     GO_process: GO:0019629 - propionate catabolic process,
                     2-methylcitrate cycle [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylisocitrate lyase"
                     /protein_id="HGU4547636.1"
                     /translation="MSLHSPGKAFRAALTKENPLQIVGTINANHALLAQRAGYQAIYL
                     SGGGVAAGSLGLPDLGISTLDDVLTDIRRITDVCSLPLLVDADIGFGSSAFNVARTVK
                     SMIKAGAAGLHIEDQVGAKRCGHRPNKAIVSKEEMVDRIRAAVDAKTDPDFVIMARTD
                     ALAVEGLDAAIERAQAYVEAGAEMLFPEAITELAMYRQFADAVQVPILANITEFGATP
                     LFTIDELRSAHVAMALYPLSAFRAMNRAAEHVYNVLRQEGTQKSVIDTMQTRNELYES
                     INYYQYEAKLDDLFARSQVK"
     gene            5569..6738
                     /gene="prpC"
                     /locus_tag="ACHOSC_RS08375"
                     /old_locus_tag="pgaptmp_001675"
     CDS             5569..6738
                     /gene="prpC"
                     /locus_tag="ACHOSC_RS08375"
                     /old_locus_tag="pgaptmp_001675"
                     /EC_number="2.3.3.16"
                     /EC_number="2.3.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050440 - 2-methylcitrate synthase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-methylcitrate synthase"
                     /protein_id="HGU4547637.1"
                     /translation="MSDTTILQNSTHVIKPKKSVALSGVPAGNTALCTVGKSGNDLHY
                     RGYDILDLAEHCEFEEVAHLLIHGKLPTRDELAAYKTKLKALRGLPANVRTVLEALPA
                     ASYPMDVMRTGVSALGCTLPEKEGHTVSGARDIADKLLASLSSILLYWYHYSHNGERI
                     QPETDDDSIGGHFLHLLHGEKPSQSWEKAMHISLVLYAEHEFNASTFTSRVIAGTGSD
                     MYSAIIGAIGALRGPKHGGANEVSLEIQQRYETPDEAEADIRKRVENKEVVIGFGHPV
                     YTIADPRHQVIKRVAKQLSQEGGSLKMYNIANRLETVMWESKKMFPNLDWFSAVSYNM
                     MGVPTEMFTPLFVIARVTGWAAHIIEQRQDNKIIRPSANYVGPEDRQFVALDKRQ"
     gene            6772..8223
                     /gene="prpD"
                     /locus_tag="ACHOSC_RS08380"
                     /old_locus_tag="pgaptmp_001676"
     CDS             6772..8223
                     /gene="prpD"
                     /locus_tag="ACHOSC_RS08380"
                     /old_locus_tag="pgaptmp_001676"
                     /EC_number="4.2.1.79"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047547 - 2-methylcitrate dehydratase
                     activity [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0019679 - propionate metabolic process,
                     methylcitrate cycle [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-methylcitrate dehydratase"
                     /protein_id="HGU4547638.1"
                     /translation="MSAQINNIRPEFDREIVDIVDYVMNYEISSKVAYDTAHYCLLDT
                     LGCGLEALEYPACKKLLGPIVPGTVVPNGVRVPGTQFQLDPVQAAFNIGAMIRWLDFN
                     DTWLAAEWGHPSDNLGGILATADWLSRNAVASGKAPLTMKQVLTAMIKAHEIQGCIAL
                     ENSFNRVGLDHVLLVKVASTAVVAEMLGLTREEILNAVSLAWVDGQSLRTYRHAPNTG
                     TRKSWAAGDATSRAVRLALMAKTGEMGYPSALTAPVWGFYDVSFKGESFRFQRPYGSY
                     VMENVLFKISFPAEFHSQTAVEAAMTLYEQMQAAGKTAADIEKVTIRTHEACIRIIDK
                     KGPLNNPADRDHCIQYMVAIPLLFGRLTAADYEDNVAQDKRIDALREKINCFEDPAFT
                     ADYHDPEKRAIANAITLEFTDGTRFEEVVVEYPIGHARRRQDGIPKLVDKFKINLARQ
                     FPTRQQQRILEVSLDRTRLEQMPVNEYLDLYVI"
     gene            8263..10149
                     /gene="prpE"
                     /locus_tag="ACHOSC_RS08385"
                     /old_locus_tag="pgaptmp_001677"
     CDS             8263..10149
                     /gene="prpE"
                     /locus_tag="ACHOSC_RS08385"
                     /old_locus_tag="pgaptmp_001677"
                     /EC_number="6.2.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050218 - propionate-CoA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0019629 - propionate catabolic process,
                     2-methylcitrate cycle [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="propionate--CoA ligase"
                     /protein_id="HGU4547639.1"
                     /translation="MSFSEFYQRSINEPEQFWAEQARRIDWQTPFTQTLDHSNPPFAR
                     WFCEGRTNLCHNAIDRWLEKQPEALALIAVSSETEEERTFTFRQLHDEVNAVASMLRS
                     LGVQRGDRVLVYMPMIAEAHITLLACARIGAIHSVVFGGFASHSVAARIDDAKPVLIV
                     SADAGARGGKIIPYKKLLDDAISQAQHQPRHVLLVDRGLAKMARVSGRDVDFASLRHQ
                     HIGARVPVAWLESNETSCILYTSGTTGKPKGVQRDVGGYAVALATSMDTIFGGKAGSV
                     FFCASDIGWVVGHSYIVYAPLLAGMATIVYEGLPTWPDCGVWWKIVEKYQVSRMFSAP
                     TAIRVLKKFPTAEIRKHDLSSLEVLYLAGEPLDEPTASWVSNTLDVPVIDNYWQTESG
                     WPIMAIARGLDDRPTRLGSPGVPMYGYNVQLLNEVTGEPCGVNEKGMLVVEGPLPPGC
                     IQTIWGDDDRFVKTYWSLFSRPVYATFDWGIRDADGYHFILGRTDDVINVAGHRLGTR
                     EIEESISSHPGVAEVAVVGVKDALKGQVAVAFVIPKESDSLEDRDVAHSQEKAIMALV
                     DSQIGNFGRPAHVWFVSQLPKTRSGKMLRRTIQAICEGRDPGDLTTIDDPASLDQIRQ
                     AMEE"
     gene            10488..11747
                     /gene="codB"
                     /locus_tag="ACHOSC_RS08390"
                     /old_locus_tag="pgaptmp_001678"
     CDS             10488..11747
                     /gene="codB"
                     /locus_tag="ACHOSC_RS08390"
                     /old_locus_tag="pgaptmp_001678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015209 - cytosine transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytosine permease"
                     /protein_id="HGU4547640.1"
                     /translation="MSQDNNFSQGPVPQSARKGVLALTFVMLGLTFFSASMWTGGTLG
                     TGLSYHDFFLAVLIGNLLLGIYTSFLGYIGAKTGLTTHLLARFSFGVKGSWLPSLLLG
                     GTQVGWFGVGVAMFAIPVGKATGLDINLLIAVSGLLMTVTVFFGISALTVLSVIAVPA
                     IACLGGYSVWLAVNGMGGLDALKAVVPAQPLDFNVALALVVGSFISAGTLTADFVRFG
                     RNAKLAVLVAMVAFFLGNSLMFIFGAAGAAALGMADISDVMIAQGLLLPAIVVLGLNI
                     WTTNDNALYASGLGFANITGMSSKTLSVINGIIGTVCALWLYNNFVGWLTFLSAAIPP
                     VGGVIIADYLMNRRRYEHFATTRMMSVNWVAILAVALGIAAGHWLPGIVPVNAVLGGA
                     LSYLILNPILNRKTTAAMTHVEVNSVE"
     gene            11737..13020
                     /gene="codA"
                     /locus_tag="ACHOSC_RS08395"
                     /old_locus_tag="pgaptmp_001679"
     CDS             11737..13020
                     /gene="codA"
                     /locus_tag="ACHOSC_RS08395"
                     /old_locus_tag="pgaptmp_001679"
                     /EC_number="3.5.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytosine/isoguanine deaminase"
                     /protein_id="HGU4547641.1"
                     /translation="MSNNALQTIINARLPGKEGLWQIHLQDGKISAIDAQSGVMPITE
                     NSLDAEQGLVIPPFVEPHIHLDTTQTAGQPNWNQSGTLFEGIERWAERKALLTHDDVK
                     QRAWQTLKWQIANGIQHVRTHVDVSDATLTALKAMLEVKQEVAPWIDLQIVAFPQEGI
                     LSYPNGEALLEEALRLGADVVGAIPHFEFTREYGVESLHKTFALAQKYDRLIDVHCDE
                     IDDEQSRFVETVAALAHREGMGARVTASHTTAMHSYNGAYTSRLFRLLKMSGINFVAN
                     PLVNIHLQGRFDTYPKRRGITRVKEMLESGINVCFGHDDVFDPWYPLGTANMLQVLHM
                     GLHVCQLMGYGQINDGLNLITHHSARTLNLQDYGIAAGNSANLIILPAENGFDALRRQ
                     VPVRYSVRGGKVIASTQPAQTTVYLEQPEAIDYKR"
     gene            complement(13060..13959)
                     /gene="cynR"
                     /locus_tag="ACHOSC_RS08400"
                     /old_locus_tag="pgaptmp_001680"
     CDS             complement(13060..13959)
                     /gene="cynR"
                     /locus_tag="ACHOSC_RS08400"
                     /old_locus_tag="pgaptmp_001680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator CynR"
                     /protein_id="HGU4547642.1"
                     /translation="MLSRHINYFLAVAEHGSFTRAASALHVSQPALSQQIRQLEESLG
                     VPLFDRSGRTIRLTDAGEVWRQYASRALQELGAGKRAIHDVADLTRGSLRIAVTPTFT
                     SYFIGPLMADFYARYPGITLQLQEMSQEKIEDLLCRDELDVGIAFAPVHSPELEAIPL
                     LTESLALVVAQHHPLAACEQVALSRLHDEKLVLLSAEFATREQIDHYCEKAGLHPQVV
                     IEANSISAVLELIRRTSLSTLLPAAIATQHDGLKAISLAPPLLERTAVLLRRKNSWQT
                     AAAKAFLHMALEECADVGENESR"
     gene            14069..14728
                     /gene="cynT"
                     /locus_tag="ACHOSC_RS08405"
                     /old_locus_tag="pgaptmp_001681"
     CDS             14069..14728
                     /gene="cynT"
                     /locus_tag="ACHOSC_RS08405"
                     /old_locus_tag="pgaptmp_001681"
                     /EC_number="4.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbonic anhydrase CynT"
                     /protein_id="HGU4547643.1"
                     /translation="MKEIIDGFLKFQREAFPKREALFKQLATQQSPRTLFISCSDSRL
                     VPELVTQREPGDLFVIRNAGNIVPSYGPEPGGVSASVEYAVAALRVSDIVICGHSNCG
                     AMTAIASCQCMDHMPAVSHWLRYADSARVVNEARPHSDLPSKAAAMVRENVIAQLANL
                     QTHPSVRLALEEGRIALHGWVYDIESGSIAAFDGATRQFVPLAANPRVCAIPLRQPTA
                     A"
     gene            14759..15229
                     /gene="cynS"
                     /locus_tag="ACHOSC_RS08410"
                     /old_locus_tag="pgaptmp_001682"
     CDS             14759..15229
                     /gene="cynS"
                     /locus_tag="ACHOSC_RS08410"
                     /old_locus_tag="pgaptmp_001682"
                     /EC_number="4.2.1.104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008824 - cyanate hydratase activity
                     [Evidence IEA];
                     GO_process: GO:0009439 - cyanate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyanase"
                     /protein_id="HGU4547644.1"
                     /translation="MIQSQINRNIRLDLADAILLSKAKKDLSFAEIADGTGLAEAFVT
                     AALLGQQALPADAARLVGAKLDLDEDAILLLQMIPLRGCIDDRIPTDPTMYRFYEMLQ
                     VYGTTLKALVHEKFGDGIISAINFKLDVKKVADPEGGERAVITLDGKYLPTKPF"
     gene            15235..16416
                     /gene="cynX"
                     /locus_tag="ACHOSC_RS08415"
                     /old_locus_tag="pgaptmp_001683"
     CDS             15235..16416
                     /gene="cynX"
                     /locus_tag="ACHOSC_RS08415"
                     /old_locus_tag="pgaptmp_001683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001306928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyanate transporter CynX"
                     /protein_id="HGU4547645.1"
                     /translation="MRNHQKTFTMLLVLVLIGLNMRPLLTSVGPLLPQLRQASGMSFS
                     VAALLTALPVVTMGGLALAGSWLHQHVSERRSVAISLLLIAVGALMRELYPQSVLLLS
                     SALLGGVGIGIIQAVMPSVIKRRFQQRTPLVMGLWSAALMGGGGLGAAITPWLVQHSE
                     TWYQTLAWWALPAVVALFAWWWQSAREVASSHKTTTTPVRVVFTPRAWTLGVYFGLIN
                     GGYASLIAWLPAFYIEIGASAQYSGSLLALMTLGQAAGALLMPAMARHQDRRKLLMLA
                     LVLQLVGFCGFIWLPLQLPVSWAMVCGLGLGGAFPLCLLLALDHSVQPAIAGKLVAFM
                     QGIGFIIAGLAPWFSGVLRSISGNYLMDWAFHALCVVGLMIITLRFAPARFPQLWVKE
                     V"
     gene            complement(16495..17748)
                     /gene="lacY"
                     /locus_tag="ACHOSC_RS08420"
                     /old_locus_tag="pgaptmp_001684"
     CDS             complement(16495..17748)
                     /gene="lacY"
                     /locus_tag="ACHOSC_RS08420"
                     /old_locus_tag="pgaptmp_001684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactose permease"
                     /protein_id="HGU4547646.1"
                     /translation="MYYLKNTNFWMFGLFFFFYFFIMGAYFPFFPIWLHDINHISKSD
                     TGIIFAAISLFSLLFQPLFGLLSDKLGLRKYLLWIITGMLVMFAPFFIFIFGPLLQYN
                     ILVGSIVGGIYLGFCFNAGAPAVEAFIEKVSRRSNFEFGRARMFGCVGWALCASIVGI
                     MFTINNQFVFWLGSGCALILAVLLFFAKTDAPSSATVANAVGANHSAFSLKLALELFR
                     QPKLWFLSLYVIGVSCTYDVFDQQFANFFTSFFATGEQGTRVFGYVTTMGELLNASIM
                     FFAPLIINRIGGKNALLLAGTIMSVRIIGSSFATSALEVVILKTLHMFEVPFLLVGCF
                     KYITSQFEVRFSATIYLVCFCFFKQLAMIFMSVLAGNMYESIGFQGAYLVLGLVALGF
                     TLISVFTLSGPGPLSLLRRQVNEVA"
     gene            complement(17800..20874)
                     /gene="lacZ"
                     /locus_tag="ACHOSC_RS08425"
                     /old_locus_tag="pgaptmp_001685"
     CDS             complement(17800..20874)
                     /gene="lacZ"
                     /locus_tag="ACHOSC_RS08425"
                     /old_locus_tag="pgaptmp_001685"
                     /EC_number="3.2.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-galactosidase"
                     /protein_id="HGU4547647.1"
                     /translation="MTMITDSLAVVLQRRDWENPGVTQLNRLAAHPPFASWRNSEEAR
                     TDRPSQQLRSLNGEWRFAWFPAPEAVPESWLECDLPEADTVVVPSNWQMHGYDAPIYT
                     NVTYPITVNPPFVPTENPTGCYSLTFNVDESWLQEGQTRIIFDGVNSAFHLWCNGRWV
                     GYGQDSRLPSEFDLSAFLRAGENRLAVMVLRWSDGSYLEDQDMWRMSGIFRDVSLLHK
                     PTTQISDFHVATRFNDDFSRAVLEAEVQMCGELRDYLRVTVSLWQGETQVASGTAPFG
                     GEIIDERGSYADRVTLRLNVENPKLWSAEIPNLYRAVVELHTADGTLIEAEACDVGFR
                     EVRIENGLLLLNGKPLLIRGVNRHEHHPLHGQVMDEQTMVQDILLMKQNNFNAVRCSH
                     YPNHPLWYTLCDRYGLYVVDEANIETHGMVPMNRLTDDPRWLPAMSERVTRMVQRDRN
                     HPSVIIWSLGNESGHGANHDALYRWIKSVDPSRPVQYEGGGADTFATDIICPMYARVD
                     EDQPFPAVPKWSIKKWLSLPGELRPLILCEYAHAMGNSLGGFAKYWQAFRQYPRLQGG
                     FVWDWVDQSLIKYDENGNPWLAYGGDFGDTPNDRQFCMNGLVFADRTPHPALTEAKYQ
                     QQFFQFRLSGQTIEVTSEYLFRHSDNELLHWMVALDGKPLASGEVPLDVAPQGKQLIE
                     LPGLPQPESAGQLWLTVHVVQPNATAWSEAGHISAWQQWRLAENLSVTLPAASHAIPH
                     LTTSEMDFCIELGNKRWQFNRQSGLLSQMWIGDEKQLLTPLRDQFTRAPLDNDIGVSE
                     ATRIDPNAWVERWKAAGHYQAEAALLQCSADTLADAVLITTAHAWQHQGKTLFISRKT
                     YRIDGSGQMAITVDVEVASDTPHPARIGLTCQLAQVAERVNWLGLGPQENYPDRLTAA
                     CFDRWDLPLSDMYTPYVFPSENGLRCGTRELNYGPHQWRGDFQFNISRYSQQQLMETS
                     HRHLLHAEEGTWLNIDGFHMGIGGDDSWSPSVSAEFQLSAGRYHYQLVWCQK"
     gene            complement(20997..22079)
                     /gene="lacI"
                     /locus_tag="ACHOSC_RS08430"
                     /old_locus_tag="pgaptmp_001686"
     CDS             complement(20997..22079)
                     /gene="lacI"
                     /locus_tag="ACHOSC_RS08430"
                     /old_locus_tag="pgaptmp_001686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414879.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional repressor LacI"
                     /protein_id="HGU4547648.1"
                     /translation="MKPVTLYDVAEYAGVSYQTVSRVVNQASHVSAKTREKVEAAMAE
                     LNYIPNRVAQQLAGKQSLLIGVATSSLALHAPSQIVAAIKSRADQLGASVVVSMVERS
                     GVEACKAAVHNLLAQRVSGLLINYPLDDQDATAVEAACANVPALFLDVSDQTPINSII
                     FSHEDGTRLGVEHLVALGHQQIALLAGPLSSVSARLRLAGWHKYLTRNQIQPIAEREG
                     DWSAMSGFQQTMQMLNEGIVPTAMLVANDQMALGAMRAITESGLRVGADISVVGYDDT
                     EDSSCYIPPLTTIKQDFRLLGQTSVDRLLQLSQGQAVKGNQLLPVSLVKRKTTLAPNT
                     QTASPRALADSLMQLARQVSRLESGQ"
     gene            complement(22156..22989)
                     /gene="mhpR"
                     /locus_tag="ACHOSC_RS08435"
                     /old_locus_tag="pgaptmp_001687"
     CDS             complement(22156..22989)
                     /gene="mhpR"
                     /locus_tag="ACHOSC_RS08435"
                     /old_locus_tag="pgaptmp_001687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414880.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator"
                     /protein_id="HGU4547649.1"
                     /translation="MQNNEQTEYKTVRGLTRGLMLLNMLNKLDGGASVGLLAELSGLH
                     RTTVRRLLETLQEEGYVRRSPSDDSFRLTIKVRQLSEGFRDEQWISALAAPLLGDLLR
                     EVVWPTDVSTLDVDAMVVRETTHRFSRLSFHRAMVGRRLPLLKTASGLTWLAFCPEQD
                     RKELIEMLASRPGDDYQLAREPLKLEAILARARKEGYGQNYRGWDQEEKIASIAVPLR
                     SEQRVIGCLNLVYMASAMTIEQAAEKHLPALQRVAKQIEEGVESQAILVAGRRSGVHL
                     R"
BASE COUNT         5414 a         6095 c         6163 g         5388 t
ORIGIN      
        1 aaattgttaa attaactccc tccagtacac aatacttcac acgttagtta tgagcgattt
       61 ctgatagtgc ctggtttaat gagagcttta ttatctgcga cgtttatttt tatttaagag
      121 agatcgcgat gatatcatta aaagctccgc acaataattt gatgccatat acgcaacaaa
      181 gcatacttaa tacggttaaa aacaatcagt taccagagga tatcaaaagc tccctggttt
      241 cctgtgtcga tatattcaag gttttgatta aacaatatta tgattatcct tatgattgtc
      301 gtgatgattt agtcgatgac gacaaactca ttcatctcat ggctgccgta cgagactgtg
      361 agtggtcaga cgataacgca ctcaccataa atgtgcagtt taatgatttc cctggatttt
      421 atgactggat ggattaccct gatcacccgg ttaagtttgt ttttcgcata cttgaaaatc
      481 aaaaagggac agtttgggta tatgaccagg atgatgcgtt tcttgatatt aaagcaaatg
      541 tgcaggctgg acgctttacc gggcttaaga aattagtgca gtttatcgat tcagtgcgaa
      601 cagattgcaa atgtattctt cttgaacacc atatgccttt gctgagaatt ttccctaaag
      661 ggaaagagtg tatgcgtgtg gaaaaatggc ttcgcgaaat gtcgtctatt ccggagacag
      721 acgcgcccat taagcaggcg cttgcgcatg gattattatt acacttaaaa aatatccacc
      781 ctgtttttcc tgaatcactg gtgatgttat tgctctctgt tctggacgta aaaacataca
      841 gagatgacgc acgattaaat gaatggatat cgaatagagt gcaggaatta ggtgacagat
      901 attatcctgt aaataagcac gttaaaatcc gctacactct ttagttcaga aagggttcca
      961 cctgcaagtt gttattccac cagagagtga atcgcaaagc aggtaatcat ttcatctgta
     1021 tatccgtatg ccaaatggca ttccaccgcc tttttcgccg atattttgtt gtgatcggcg
     1081 acgcttcgct taaaaaagca ccagcagtgg tttcgcagct atgcggtgta taaaaaatga
     1141 tctcatgcag atgttttgtg aatgtgtggg ttgacattca tataaaaaaa atcataattc
     1201 catcatgttt gtgtggggtc ttttctgtac cttagcatcg cactcaagcc tgacagaaaa
     1261 tatgctgtaa ggctcatatc aaaaaccgcc attagctcat caggaagagc agacgacaac
     1321 caggattgtt gtatggtacg gggttcgagg cctcgatggc ggtccaactt ttcaaaactc
     1381 ttctgaatca gatcccctta ttatcatccc tgcaaagtgg aacattgcca ttgtttggct
     1441 attcaggacg ggtcagacca atgaaaaaag cccacacagg ggagagtggg ctgaaatggg
     1501 aagctaaaga ctcaagtaaa cttatcggaa ataaggacca cgcattacgg gggcaatcat
     1561 cgccgatggt cgggattcgg caaatcaggt tgattacgta gagagtaaat tatctgctca
     1621 ccgctgcgtc accccttcgt aaatcaggcg tagcgcgaat acaccaataa ttgcaccaat
     1681 aacccgactg gcaacgcgtt gcatacgccc ataagcacga cgcacagcgg gcaaagaaaa
     1741 cgcctgacta agaaaaactc gccagataat tgatgcgagc acaatccccg cccaggccat
     1801 taaacgtgcc catgttggtg tttcggcatt taatgttact gagaaaatac tgataaaaaa
     1861 taaaacggtt tgcgggttag agagatcggt aattaatccg cggcgaaaaa agacatacca
     1921 gggggcgcta atcggttgtt gtagtgtgct catttgcggt gttgactggc ggcgcatgct
     1981 gcaccacgca aaccataaga gataagcgcc gccgacgatt ctgataagcg aaaaaatctc
     2041 ctcacactgc gtaattagcg ttgcaagacc aaacaaaccc aacccggaat aaaatgcatc
     2101 gcccagcgcc acgcccagcc cggtcagcac ccctgcgcgt cgaccggaag ccaggctggt
     2161 ttgtactacc acaaagagat tggctcccgg attaaaaaaa gtaatcacga acagtcctac
     2221 ggtcaggtaa acggcgtgca aaggatccat agtgatttca tccataaata agtgaagtaa
     2281 cttcatcata tttctaccaa aaataatcgg gtgcgagaga gatcacaaag tgtcttattt
     2341 ccggttactg gcgtttatgc cctgactgaa ctaattatta atcaacccaa taatgtgggt
     2401 ggatgatagt gtgataacaa ctctggagcc gtaatatgaa aataatctct aaaatgttag
     2461 tcggtgcgtt agcgttagcc gttaccaatg tctatgccgc tgaattgatg accaaagcgg
     2521 aatttgaaaa agttgaatcg cagtatgaaa aaataggtga tatttcaact agcaatgaaa
     2581 tgtcgactgc agacgcgaaa gaagatttga tcaaaaaagc ggatgaaaaa ggggctgatg
     2641 tgttggtact gacctccggt caaactgaca ataagatcca cggcacggca aatatttata
     2701 agaagaagta attctgaacc ctatgtaaac atgcccgatg cgtaagttta tcggtgatca
     2761 tctattgaaa tttatgccgg ataaagcgtt cgcgctgcat tcggcagttc agcttttcag
     2821 ccgccgccag aacgtcgtcc ggctgatgcc taaataattc gccgctgctg ttttatcgcc
     2881 attaaatttc tccagtgcct gttgtggtgt cagtaagcgt ggagcgggag ttttcgccga
     2941 ctcgcgcgcc agttccggca gtagcagctg caaaaattgc ggcgttaaat ccggcgtcgg
     3001 ttccacactt aaaaatagcg ccagtcgctc catcatattg cgcagttcac gaatattgcc
     3061 cggccagtcg tagtgcacca gcacggtttc gcttgcctgt aatcccaggc gtaatgcggc
     3121 agaaaacggg gcggagagcg ccgccagaga cactttcaaa aagctttccg ccagcggcag
     3181 aatatccgcc acccgctcac gcagtggtgg cagttgcagg cgtaaaatgc tcagccgata
     3241 aaacaggtca cggcgaaact gcccttgccg catatcttct tccagcttgc agtgagtggc
     3301 gctaatgacc cgcacatcca ccggaacagg ctgatgcccg ccgacgcggg taacctcttt
     3361 ttcttccagc acccgcagca gccgggtctg caaaggtagc ggcatttcgc caatctcatc
     3421 caaaaacagc gtgccaccgt gggcgatttc aaacagcccg gcgcgacctc cgcgtcgcga
     3481 gccggtaaac gccccttcct catagccaaa cagctccgct tccagcagcg attcggcaat
     3541 cgccccgcag ttgacggcaa caaacggatg cgactttttg ccctgtcgcg catcgtggcg
     3601 ggcaaagtat tcccgatgaa ttgcctgggc tgccagttct ttgcccgtcc ccgtttcccc
     3661 ctcaatcaac accgccgcac tggagcgggc atacagcaaa atagtctgcc gcacctgttc
     3721 catctgtggt gattgaccga gcatatcgcc cagcacgtaa cgagtacgca gggcgttgcg
     3781 agtggcatcg tgagtgttat ggcgtaacga catgcgcgtc atatccagcg catcgctgaa
     3841 cgcctggcgc acggtggcgg cggaatagat aaaaattccg gtcattccgg cttcttctgc
     3901 cagatcggta atcagccccg cgccgaccac cgcttcggtg ccgttagctt ttagctcgtt
     3961 aatctgcccg cgtgcgtctt cttcggtaat gtagctacgt tgatcgaggc gcaaattaaa
     4021 ggttttttga aacgccacca gtgccggaat agtttcctga taagtgacaa cgccgataga
     4081 agaggtgagt tttccggctt ttgccagtgc ctgtaacaca tcgtagccgc tcggtttaat
     4141 caaaataact ggcactgaca ggcggctttt caggtacgcg ccgttagatc cagccgcgat
     4201 gatggcgtca cagcgttcgt ttgccagttt cttgtggatg taggtcaccg ctttttcaaa
     4261 gccaagctgg ataggggtaa tgttcgccag gtgatcaaac tcgaggctga tatcgcgaaa
     4321 cagctcgaac aggcgcgtta cagataccgt ccagataacc ggtttgtcgt cattaagccg
     4381 tggtggatgt gccatagcgc accgcaaagt taagaaaccg aatattgggt ttagtcttgt
     4441 ttcataattg ttgcaatgaa acgcggtgaa acattgcctg aaacgttaac tgaaacgcat
     4501 atttgcggat tagttcatga ctttatctct aacaaattga aattaaacat ttaattttat
     4561 taaggcaatt gtggcacacc ccttgctttg tctttatcaa cgcaaataac aagttgataa
     4621 caaaggatgg gctatgtctc tacactctcc aggtaaagcg tttcgcgctg cactgactaa
     4681 agaaaatcca ttgcagattg ttggcaccat caacgctaat catgcgctgt tggcgcagcg
     4741 tgccggatat caggcaattt atctttctgg cggtggcgtg gcggcaggtt cgctggggct
     4801 gcccgatctc ggtatttcta ctcttgatga cgtgctgacc gatattcgcc gtatcaccga
     4861 cgtttgttcg ctgccgctgc tggtggatgc ggatatcggt tttggttctt cggcctttaa
     4921 cgtggcgcgc accgtgaaat cgatgattaa agccggtgcg gcaggattgc atattgaaga
     4981 tcaggttggt gcgaaacgct gcggtcatcg tccgaataaa gcgatcgtct cgaaagaaga
     5041 gatggtggat cggatccgcg cggcggtgga tgcgaaaacc gatcctgatt ttgtgatcat
     5101 ggcgcgcacc gatgctctgg cggtagaggg gctggatgcg gcgatcgagc gtgcgcaggc
     5161 ctatgttgaa gcgggtgccg agatgttgtt cccggaggcg attaccgaac tcgccatgta
     5221 ccgccagttt gccgatgcgg tgcaggtgcc aatcctcgcc aacattaccg aatttggtgc
     5281 cacgccgctg tttaccatcg acgaattacg cagcgcccat gtcgcaatgg cgctgtaccc
     5341 gctttcagcg ttccgcgcca tgaaccgcgc cgctgagcat gtctacaacg tcctgcgtca
     5401 ggaaggcacg cagaaaagcg tcatcgatac catgcaaacc cgcaacgagc tgtacgaaag
     5461 catcaactac taccagtacg aagcgaagct cgacgacctg tttgcccgta gccaggtgaa
     5521 ataaaacaca acgaaaaata tctcgaccct acaaatgacg aggacaacat gagcgacaca
     5581 acgatcctgc aaaacagtac ccatgtcatt aaaccgaaaa aatctgtggc actttctggc
     5641 gttccggcgg gcaatacggc gctctgcacc gtgggtaaaa gtggcaatga cctgcattac
     5701 cgtggctacg atattcttga tctggcggaa cattgcgaat ttgaagaagt ggcgcacctg
     5761 ctgatccacg gcaaactgcc gacccgtgac gaactcgccg cttacaaaac gaaactgaaa
     5821 gccctgcgcg gtttaccggc taacgtgcgt accgtgctgg aagccttacc ggcggcgtcg
     5881 tacccgatgg atgtcatgcg caccggcgtt tccgcgctcg gctgcacact gccagaaaaa
     5941 gaggggcaca ctgtctctgg cgcgcgggat attgccgaca aactgctggc atcacttagc
     6001 tcgattctcc tttattggta tcactacagt cacaacggcg aacgcatcca gccggaaacc
     6061 gatgacgact ccatcggtgg tcacttcctg catctgctgc acggcgaaaa gccatcgcaa
     6121 agctgggaaa aggcgatgca tatttcgcta gtgctgtacg ccgaacacga gtttaacgcc
     6181 tccaccttta ccagtcgggt gattgcgggc actggctctg atatgtattc cgcgattatt
     6241 ggcgcgattg gcgcactgcg cgggccgaaa cacggcggag cgaatgaagt gtcgctggag
     6301 atccagcaac gctacgaaac gccggacgaa gccgaagccg atatccgcaa gcgggtggaa
     6361 aacaaagaag tggtcattgg ttttggtcat ccggtttata ccatcgccga cccgcgccac
     6421 caggtgatca aacgtgtggc gaagcagctc tcgcaggaag gcggctcgtt gaagatgtac
     6481 aacatcgcca atcgcctgga aacggtgatg tgggagagca aaaagatgtt ccccaatctc
     6541 gactggttct ctgctgtttc ctacaacatg atgggcgttc ccaccgagat gttcacacca
     6601 ctgtttgtca tcgcccgtgt caccggctgg gcggcgcaca tcattgaaca acgtcaggac
     6661 aacaaaatta tccgtccttc cgccaattat gttggaccgg aagaccgcca gtttgtcgcg
     6721 ctggataagc gccagtaaac ctctacgaat aacaataagg aaacataccc aatgtcagct
     6781 caaatcaaca acatccgccc ggaatttgat cgtgaaatcg ttgatatcgt cgattacgtg
     6841 atgaactacg aaatcagctc taaagtggcc tacgacaccg cacattactg cctgctcgac
     6901 acgctcggct gcggtctgga agctctcgaa tacccggcct gtaaaaaact gctggggcca
     6961 attgttcctg gcaccgtcgt acccaacggc gtgcgcgtcc ccggaactca gtttcagctc
     7021 gaccctgtcc aggcggcatt taacattggc gcgatgatcc gctggctcga tttcaacgat
     7081 acctggctgg cggcggagtg ggggcatcct tccgacaacc tcggcggcat tctggcaacg
     7141 gcagactggc tttcgcgcaa cgctgtcgcc agcggcaaag cgccgttgac catgaaacag
     7201 gtgctgaccg caatgatcaa agcccatgaa attcagggct gcatcgcgct ggaaaactcc
     7261 tttaaccgcg tcggcctcga ccacgttctg ttagtgaaag tggcttccac cgccgtggtc
     7321 gccgaaatgc tcggcctgac ccgcgaggaa attctcaacg ccgtttcgct ggcgtgggtg
     7381 gacggtcagt cgctgcgcac ctatcgccat gcgccgaaca ccggcacgcg taaatcctgg
     7441 gcggcgggcg atgccacttc ccgcgcggta cgtctggcac tgatggcgaa aacgggcgaa
     7501 atgggttacc cgtcagccct gaccgcgccg gtatggggct tctacgacgt ctcctttaaa
     7561 ggtgaatcgt tccgcttcca gcgtccgtac ggttcttacg tcatggagaa tgtgctgttc
     7621 aaaatctcct tcccggcgga gttccactcc cagacggcag ttgaagcggc gatgacgctc
     7681 tatgaacaga tgcaggcagc aggcaagacg gcggcggata tcgaaaaagt gaccattcgc
     7741 acccacgaag cctgtattcg catcatcgac aaaaaggggc cgctcaataa cccggctgac
     7801 cgcgaccact gcattcagta catggtggcg atcccgctgt tattcgggcg cttaacggcg
     7861 gcagattacg aggacaacgt tgcgcaagat aaacgcatcg acgccctgcg cgagaagatc
     7921 aattgctttg aagatccggc atttaccgct gactaccacg acccggaaaa acgcgccatc
     7981 gccaatgcca ttacccttga gttcaccgac ggcacacgat ttgaagaagt ggtggtggag
     8041 taccccattg gtcatgctcg ccgccgtcag gatggtattc cgaaactggt cgataaattc
     8101 aaaatcaatc tcgcgcgcca gttcccgact cgccaacagc agcgcattct ggaggtttct
     8161 ctcgacagaa ctcgcctgga acagatgccg gtcaatgagt atctcgacct gtacgtcatt
     8221 taagtaaact gcggtaaggc gtaagttcaa caggagagca ttatgtcttt tagcgaattt
     8281 tatcagcgtt cgattaacga accggagcag ttctgggccg agcaggcccg gcgtattgac
     8341 tggcagacgc cctttacgca aacgctcgac cacagcaatc cgccgtttgc ccgttggttt
     8401 tgtgaaggcc gaaccaattt gtgccacaac gctatcgacc gctggctgga gaaacagcca
     8461 gaggcactgg cattgattgc cgtctcttcg gaaacagaag aagagcgcac ctttaccttt
     8521 cgccagctgc atgacgaagt gaacgcggtg gcctcaatgt tgcgctcact gggcgtgcag
     8581 cgcggcgatc gggtgctggt gtatatgccg atgattgccg aagcgcatat tactctgctg
     8641 gcctgcgcgc gcattggtgc tattcattcg gtggtatttg gtggttttgc ctcgcacagc
     8701 gtggcggcgc gaattgatga cgctaaaccg gtgctgattg tctcggctga tgccggagcg
     8761 cgcggtggca aaatcattcc ctataaaaaa ttgctcgacg atgcgataag tcaggcgcag
     8821 caccagccac gccatgtttt gctggtggat cgcgggctgg cgaaaatggc gcgcgtcagc
     8881 gggcgggatg tcgatttcgc gtcgttgcgc catcaacaca tcggcgcgcg ggtaccggtg
     8941 gcgtggctgg aatccaacga aacctcctgc attctctaca cttccggcac gaccggcaaa
     9001 cctaaaggcg tgcagcgtga cgtcggcgga tatgcggtgg cgctggcgac ctcgatggac
     9061 accatttttg gcggcaaagc gggcagcgtg ttcttttgcg catcggatat cggctgggtg
     9121 gtggggcatt cgtatatcgt ttacgcgccg ctgctggcgg ggatggcgac tatcgtttac
     9181 gaaggattgc cgacctggcc ggactgcggc gtgtggtgga aaatcgtcga gaaatatcag
     9241 gttagccgga tgttctcagc gccgaccgcc attcgcgtgc tgaaaaaatt ccctaccgct
     9301 gaaattcgca aacacgatct ctcgtcgctg gaagtgctct atctggctgg agaaccgctg
     9361 gacgagccga ccgccagttg ggtgagcaat acgctggatg tgccggtcat cgacaactac
     9421 tggcagaccg aatccggctg gccgattatg gcgattgctc gcggtctgga cgacaggccg
     9481 acgcgtctgg gaagccccgg cgtgccgatg tatggctata acgtgcagtt gcttaatgaa
     9541 gtcaccggcg aaccgtgtgg cgtcaacgag aaagggatgc tggtggtgga agggccgctg
     9601 ccgccgggct gtattcagac catctggggc gacgacgacc gctttgtgaa gacttactgg
     9661 tcgctgtttt cccgcccggt gtacgccacc tttgactggg gcatccgtga cgctgacggt
     9721 tatcacttta ttctcgggcg cactgacgat gtaattaacg ttgccgggca tcgtctgggc
     9781 acgcgcgaga ttgaagagag tatctccagc catccgggcg ttgccgaagt ggcggtggtt
     9841 ggggtgaaag atgcgctgaa agggcaggtg gcggtggcgt ttgtcattcc gaaagagagc
     9901 gatagtctgg aagatcgtga tgtggcgcat tcgcaagaga aggcgattat ggcgctggtg
     9961 gacagccaga ttggcaactt tggtcgcccg gcgcacgtct ggtttgtctc gcaattgccg
    10021 aaaacgcgat ccggaaaaat gctgcgccgc acgatccagg caatttgcga aggacgcgat
    10081 cctggggatc tgacgaccat tgatgatcct gcgtcattgg atcagatccg ccaggcgatg
    10141 gaagagtaag gtattgtcgg atgcgtcgcg tggcgcatcc gacaccatgc gccgatgcct
    10201 gatgcgacgc ttaccgcgtc ttattatgcc tacaggcctg aaccgtaggt cggataaggc
    10261 gctcgcgtcg catccgacac cccgcgccga tgcctgatgc gacgctgacg cgtcttatca
    10321 gtatctaccc actacccact gtttttacac cgataatttt tcccccacct ttttgcactc
    10381 attcatataa aaaatatatt tccccacgaa aacgattgct ttttatcttc agatgaatag
    10441 aatacggcgg attttttggg tttcaaacag caaaaagggg gaatttcgtg tcgcaagata
    10501 acaactttag ccaggggcca gtcccgcagt cggcgcggaa aggggtattg gcattgacgt
    10561 tcgtcatgct gggattaacc ttcttttccg ccagtatgtg gaccggcggc actctcggaa
    10621 ccggtcttag ctatcatgat ttcttcctcg cagttctcat cggtaatctt ctcctcggta
    10681 tttacacttc atttctcggt tacattggcg caaaaaccgg cctgaccact catcttcttg
    10741 ctcgcttctc gtttggtgtt aaaggctcat ggctgccttc actgctactg ggcggaactc
    10801 aggttggctg gtttggcgtc ggtgtggcga tgtttgccat tccggtgggt aaggcaaccg
    10861 ggctggatat taatttgctg attgccgttt ccggtttact gatgaccgtc accgtctttt
    10921 ttggcatttc ggcgctgacg gttctttcgg tgattgcggt tccggctatc gcctgcctgg
    10981 gcggttattc cgtgtggctg gctgttaacg gcatgggcgg cctggacgca ttaaaagcgg
    11041 tcgttcccgc acaaccgtta gatttcaatg tcgcgctggc gctggttgtg gggtcattta
    11101 tcagtgcggg tacgctcacc gctgactttg tccggtttgg tcgcaatgcc aaactggcgg
    11161 tgctggtggc gatggtggcc tttttcctcg gcaactcgtt gatgtttatt ttcggtgcag
    11221 cgggcgctgc ggcactgggc atggcggata tctctgatgt gatgattgct cagggcctgc
    11281 tgctgcctgc gattgtggtg ctggggctga atatctggac caccaacgat aacgcactct
    11341 atgcgtcggg tttaggtttc gccaacatta ccggtatgtc gagcaaaacc ctttcggtaa
    11401 tcaacggtat tatcggtacg gtctgcgcat tatggctgta taacaatttt gtcggctggt
    11461 tgaccttcct ttcggcagct attcctccag tagggggcgt gatcatcgcc gactatctga
    11521 tgaaccgtcg ccgctatgag cactttgcga ccacgcgtat gatgagtgtc aattgggtgg
    11581 cgattctggc ggtcgcgctg gggattgccg caggccactg gttaccggga attgttccgg
    11641 tcaacgcggt attaggtggc gcgctgagct atctgatcct taacccgatt ttgaatcgta
    11701 aaacgacagc agcaatgacg catgtggagg ttaacagtgt cgaataacgc tttacaaaca
    11761 attattaacg cccggttacc aggcaaagag gggctgtggc agattcatct gcaggacgga
    11821 aaaatcagcg ccattgatgc gcaatccggc gtgatgccca taactgaaaa cagcctggat
    11881 gccgaacaag gtttagttat accgccgttt gtggagccgc atattcacct ggacaccacg
    11941 caaaccgccg gacaaccgaa ctggaatcag tccggcacgc tgtttgaagg cattgaacgc
    12001 tgggccgagc gcaaagcgtt attaacccat gacgatgtga aacaacgcgc ctggcaaacg
    12061 ctgaaatggc agattgccaa cggcattcag catgtgcgta cccatgtcga tgtttcggat
    12121 gcaacgctaa ctgcgctgaa agcaatgctg gaagtgaagc aggaagtcgc gccgtggatt
    12181 gatctgcaaa ttgtcgcctt ccctcaggaa gggattttgt cgtatcccaa cggtgaagcg
    12241 ttgctggaag aggcgttacg cttaggggca gatgtagtgg gggcgattcc gcattttgaa
    12301 tttacccgtg aatacggcgt ggagtcgctg cataaaacct tcgccctggc gcaaaaatac
    12361 gaccgtctca tcgacgttca ctgtgatgag atcgatgacg agcagtcgcg ctttgtcgaa
    12421 accgttgctg ccctggcgca ccgtgaaggc atgggcgcgc gagtcaccgc cagccacacc
    12481 acggcaatgc actcctataa cggggcgtat acctcacgcc tgttccgctt gctgaaaatg
    12541 tccggtatta actttgtcgc caacccgctg gtcaatattc atctgcaagg acgtttcgat
    12601 acgtatccaa aacgtcgcgg catcacgcgc gttaaagaga tgctggagtc cggcattaac
    12661 gtctgctttg gtcacgatga tgtcttcgat ccgtggtatc cgctgggaac ggcgaatatg
    12721 ctgcaagtgc tgcatatggg gctgcatgtt tgccagttga tgggctacgg gcagattaac
    12781 gatggcctga atttaatcac ccaccacagt gccagaacat tgaatttgca ggattacggc
    12841 attgccgccg gaaacagcgc aaacctgatt atcctgccgg ctgaaaatgg atttgatgcg
    12901 ctgcgccgtc aggttccggt acgttattcg gtacgtggcg gcaaggtgat tgccagtaca
    12961 caaccggcac aaaccaccgt atatctggag cagccagaag ccatcgatta caaacggtaa
    13021 tttcctctgt gttgaagagc cactgacctg tgtcagtggc taccgtgatt cattctcgcc
    13081 aacatccgca cattcctcca acgccatgtg caaaaatgcc ttcgcggcgg ctgtctgcca
    13141 gctatttttc cgccgcaaca aaaccgccgt tctctccagt agtggcgggg caagagaaat
    13201 cgctttaagc ccgtcatgtt gtgtggcaat cgctgctggt aacaatgtgg aaagggaagt
    13261 gcggcgaatc agctccagaa ccgcgctaat tgagttcgcc tcaatgacca cctgtggatg
    13321 tagccccgct ttctcgcagt agtggtcaat ttgctctctg gtggcaaatt ccgcgctgag
    13381 caggaccagt ttttcatcat gcaagcgact caacgccacc tgttcacagg cagccagcgg
    13441 atgatgttgc gccacgacta acgctaaact ttctgtcagt aaaggaattg cctccagctc
    13501 cggcgaatgc acaggcgcga aggcaatccc aacgtccagc tcgtcgcggc aaagcagatc
    13561 ttcgattttc tcctgcgaca tttcctgtag ctggagcgtg atgccggggt agcgcgcata
    13621 gaaatccgcc attaaagggc cgataaagta gcttgtaaag gtgggggtga cggcgatacg
    13681 cagcgatcct cgcgtcagat cggcaacatc atgaatcgcc cgtttacccg ctcccagttc
    13741 ctgtaacgcc cggctggcgt actgtcgcca gacttctcct gcatcggtga gacgaatcgt
    13801 tcgcccgcta cggtcaaaca gcggcacgcc taaactctcc tctaactggc gaatctgctg
    13861 ggaaagcgca ggctgagaga cgtgcaacgc actggcggca cgggtgaagc tgccatgttc
    13921 agccacggca agaaaataat tgatatgtcg agagagcatt cgccaaccta taagtaaatc
    13981 caatagaact catcataaat gagactttta ccttatgaca atcggcgagt agtctgcctc
    14041 tcattccaga gacagacaga ggttaacggt gaaagagatt attgatggat tccttaaatt
    14101 ccagcgcgag gcatttccga agcgggaagc cttgtttaaa cagctggcga cacagcaaag
    14161 cccgcgcaca ctttttatct cctgctccga cagccgtctg gtccctgagc tggtgacgca
    14221 acgtgagcct ggcgatctgt tcgttattcg caacgcgggc aatatcgtcc cttcctacgg
    14281 gccggaaccc ggtggcgttt ctgcttcggt ggagtatgcc gtcgctgcgc ttcgggtatc
    14341 tgacattgtg atttgtggtc attccaactg tggcgcgatg accgccattg ccagctgtca
    14401 gtgcatggac catatgcctg ccgtctccca ctggctgcgt tatgccgatt cagcccgcgt
    14461 cgttaatgag gcgcgcccgc attccgattt accgtcaaaa gctgcggcga tggtacgtga
    14521 aaacgtcatt gctcagttgg ctaatttgca aactcatcca tcggtgcgcc tggcgctcga
    14581 agaggggcgg atcgccctgc acggctgggt ctacgacatt gaaagcggca gcatcgcagc
    14641 ttttgacggc gcaacccgcc agtttgtgcc actggccgct aatcctcgcg tttgtgccat
    14701 accgctacgc caaccgaccg cagcgtaacc ttatttttaa accatcagga gatccaccat
    14761 gattcagtca caaattaacc gcaatattcg tcttgatctt gccgatgcca ttttgctcag
    14821 caaagctaaa aaagatctct catttgccga gattgccgac ggtaccggtc tggcagaagc
    14881 cttcgtaacc gcggctttgc tggggcagca agcgcttcct gccgacgccg cccgcctggt
    14941 cggggcgaag ctggatctcg acgaagacgc cattctactg ttgcagatga ttccactgcg
    15001 tggctgcatt gatgaccgta ttccaaccga cccaacgatg tatcgtttct atgaaatgtt
    15061 gcaggtgtac ggcacaaccc tgaaagcgtt ggttcatgag aagtttggcg atggcattat
    15121 cagcgcgatt aacttcaaac tcgacgttaa gaaagtggcg gacccggaag gtggcgaacg
    15181 tgcggtcatt accttagatg gtaaatatct gccgaccaag ccgttctgac agccatgcgc
    15241 aatcatcaaa agacgttcac gatgctgctg gttctggtgc tgattggtct taatatgcga
    15301 ccactgctca cctccgtcgg gccactgcta ccgcaattgc gccaggcgag cggaatgagc
    15361 tttagcgtgg ctgccctgtt gaccgctctt ccggtggtca ccatgggcgg gctggcgctg
    15421 gccggaagct ggcttcatca gcatgtcagc gaacgtcgca gtgtcgccat cagtctgttg
    15481 ctgattgccg tcggtgcatt gatgcgtgag ctttaccccc aaagtgtgct gctgcttagc
    15541 agcgcactgc ttggtggggt ggggatcggt atcattcagg cggtgatgcc ttcagtgatt
    15601 aaacggcggt ttcagcaacg cacgccactg gtgatggggc tgtggtctgc ggctctgatg
    15661 ggcggcggtg gacttggtgc cgccataact ccctggttag ttcaacatag cgaaacctgg
    15721 tatcaaacac tcgcctggtg ggcgctgcct gccgttgttg cgctctttgc ctggtggtgg
    15781 caaagcgccc gcgaggtcgc ctcttcccac aagacaacaa ccactccggt tcgcgtggta
    15841 ttcactcccc gcgcgtggac gctgggtgtt tacttcggtc tgattaacgg cggttacgcc
    15901 agcctgattg cctggttacc cgctttctat attgagattg gtgccagcgc gcagtacagc
    15961 ggttccttac tggcattgat gacgcttggg caagccgcag gagctttgct gatgcctgct
    16021 atggctcgcc atcaggatcg gcgcaaactg ttaatgctgg cgctggtgtt acaactggtg
    16081 gggttctgcg gctttatctg gttgccgctg caattgccgg tatcgtgggc gatggtgtgt
    16141 gggttaggtc tgggcggcgc gtttccgctc tgtttgctgc tggcgctcga tcactctgtg
    16201 caaccggcta ttgctggcaa gctggtggcg tttatgcagg ggatcggttt tatcatcgcc
    16261 gggcttgccc cgtggttttc tggcgtgctg cgtagtatca gcggcaatta cctgatggac
    16321 tgggcgtttc atgcgctgtg cgtcgttggg ctgatgatca taaccctgcg ttttgcacca
    16381 gcacgttttc cgcagctgtg ggtcaaagag gtatgatgcg acgcttgttt cgccagatga
    16441 tggagctata aataattgtc ggatgtgccc agtggcgcat ccgacattaa ttgcttaagc
    16501 gacttcattc acctgacgac gcagcaggga aagcgggccg gggccgctaa gcgtgaacac
    16561 ggaaattaag gtgaagccca gcgccaccag acccagcacc agataagcgc cctggaaacc
    16621 gatgctttca tacatattgc ccgccagtac agacataaaa atcatcgcca gttgcttaaa
    16681 gaagcagaaa cagaccagat aaatcgtcgc tgaaaaacgc acttcaaact ggctggtaat
    16741 atatttaaag cagcccacca gcaggaacgg tacttcaaac atatgcagcg ttttcagaat
    16801 aaccacttcc agcgctgagg tggcgaacga tgagccaata atacgtacag acataatagt
    16861 gccagccagc agcagggcgt ttttcccacc gatgcgatta atgatcagtg gcgcaaagaa
    16921 cataatcgag gcgttaagta attcgcccat tgtcgttacg tagccaaata cccgcgtacc
    16981 ctgttcaccg gtagcaaaga acgaagtaaa gaaattagca aactgttggt caaaaacatc
    17041 gtaggtgcag gaaacgccaa taacatacag tgacaaaaac cacagttttg gctgtctgaa
    17101 cagttccagt gccagcttaa ggctaaatgc cgaatggttg gcacctaccg cattggcaac
    17161 cgtggcggaa gagggcgcat ccgttttggc gaaaaagagt aaaacggcga ggatgagtgc
    17221 acagccagaa cccagccaga aaacaaactg attattgatg gtgaacatga tgccgacaat
    17281 cgaggcacac aacgcccagc caacacagcc aaacatccgc gcgcgaccaa attcgaaatt
    17341 actgcgacgg ctgactttct caataaatgc ctctactgct ggcgcaccgg cgttaaaaca
    17401 aaagcctaga taaataccac caacaatcga tcctactaaa atgttgtatt gtaacagtgg
    17461 cccgaagata aaaataaaga acggcgcaaa catcactaac atgccggtaa taatccacag
    17521 caggtatttg cgcagcccga gtttgtcaga aagcagacca aacagcggtt ggaataatag
    17581 cgagaacaga gaaatagcgg caaaaataat acccgtatca cttttgctga tatggttgat
    17641 gtcatgtagc caaatcggga aaaacgggaa gtaggctccc atgataaaaa agtaaaagaa
    17701 aaagaataaa ccgaacatcc aaaagtttgt gttttttaaa tagtacataa tggatttcct
    17761 tacgcgaaat acgggcagac atggcctgcc cggttattat tatttttgac accagaccaa
    17821 ctggtaatgg tagcgaccgg cgctcagctg gaattccgcc gacactgacg ggctccagga
    17881 gtcgtcgcca ccaatcccca tatggaaacc gtcgatattc agccatgtgc cttcttccgc
    17941 gtgcagcaga tggcgatggc tggtttccat cagttgctgt tgactgtagc ggctgatgtt
    18001 gaactggaag tcgccgcgcc actggtgtgg gccataattc aattcgcgcg tcccgcagcg
    18061 cagaccgttt tcgctcggga agacgtacgg ggtatacatg tctgacaatg gcagatccca
    18121 gcggtcaaaa caggccgcag taaggcggtc gggatagttt tcttgcggcc ctaatccgag
    18181 ccagtttacc cgctctgcga cctgcgccag ctggcaggtc aggccaatcc gtgccggatg
    18241 cggtgtatcg ctcgctactt caacatcaac ggtaatcgcc atttgaccac taccatcaat
    18301 ccggtaggtt ttccggctga taaataaggt ttttccctga tgctgccacg cgtgagcggt
    18361 cgtaatcagc accgcgtcgg caagtgtatc tgccgagcac tgcaacaacg ctgcttcggc
    18421 ctggtaatgg cccgccgcct tccagcgttc gacccaggcg ttagggtcaa tgcgggtcgc
    18481 ttcacttacg ccaatgtcgt tatccagcgg tgcacgggtg aactgatcgc gcagcggcgt
    18541 cagcagttgt ttttcatcgc caatccacat ctgtgaaaga aggcctgact gacggttaaa
    18601 ttgccaacgc ttattaccca gctcgatgca aaaatccatt tcgctggtgg tcagatgcgg
    18661 gatggcgtgg gacgcggcgg ggagtgtcac gctgaggttt tccgccagac gccactgctg
    18721 ccaggcgctg atgtgtccgg cttctgacca tgcggtcgcg ttcggttgca ctacgtgtac
    18781 cgtaagccag agttgtccgg cgctctccgg ctgcggtagt ccaggcagtt caatcaactg
    18841 tttaccttgt ggagcgacat ccagaggcac ttcaccgctt gccagcggct tgccatccag
    18901 cgccaccatc cagtgcagga gctcgttatc gctatgacgg aacaggtatt cgctggtcac
    18961 ttcgatggtt tgcccggata aacggaactg gaaaaactgc tgctggtatt ttgcttccgt
    19021 cagcgccgga tgcggcgtgc ggtcggcaaa gaccagaccg ttcatgcaga actggcgatc
    19081 gttcggcgta tcgccaaaat caccgccgta agccaaccac gggttgccgt tttcatcata
    19141 tttaatcagc gactgatcca cccagtccca gacgaagccg ccctgtaaac ggggatactg
    19201 acgaaacgcc tgccagtatt tagcgaaacc gccaagactg ttacccattg cgtgggcata
    19261 ttcgcaaagg atcagtgggc gcagttctcc aggcagcgaa agccattttt tgatggacca
    19321 tttcggcacc gccgggaagg gttggtcttc atccacgcgc gcgtacatcg ggcaaataat
    19381 atcggttgcg aaggtgtcgg ctccgccgcc ttcatactgc accgggcggg aaggatcgac
    19441 agatttgatc cagcgataca gcgcgtcgtg attagcgccg tggcctgact cattccccag
    19501 cgaccagatg atcacactcg ggtgattacg atcgcgctgc accattcgcg ttacgcgttc
    19561 gctcatcgcc ggtagccagc gcggatcatc ggtcagacga ttcattggca ccatgccgtg
    19621 ggtttcaata ttggcttcat ccaccacata caggccgtag cggtcgcaca gcgtgtacca
    19681 cagcggatgg ttcggataat gcgaacagcg cacggcgtta aagttgttct gcttcatcag
    19741 caggatatcc tgcaccatcg tctgctcatc catgacctga ccatgcagag gatgatgctc
    19801 gtgacggtta acgcctcgaa tcagcaacgg cttgccgttc agcagcagca gaccattttc
    19861 aatccgcacc tcgcggaaac cgacatcgca ggcttctgct tcaatcagcg tgccgtcggc
    19921 ggtgtgcagt tcaaccaccg cacgatagag attcgggatt tcggcgctcc acagtttcgg
    19981 gttttcgacg ttcagacgta gtgtgacgcg atcggcataa ctaccacgct catcgataat
    20041 ttcaccgccg aaaggcgcgg tgccgctggc gacctgcgtt tcaccttgcc ataaagaaac
    20101 tgttacccgt aggtagtcac gcaactcgcc gcacatctga acttcagcct ccagtacagc
    20161 gcggctgaaa tcatcattaa agcgagtggc aacatggaaa tcgctgattt gtgtagtcgg
    20221 tttatgcagc aacgagacgt cacggaaaat gccgctcatc cgccacatat cctgatcttc
    20281 cagataactg ccgtcactcc agcgcagcac catcaccgcg aggcggtttt ctccggcgcg
    20341 taaaaatgcg ctcaggtcaa attcagacgg caaacgactg tcctgaccgt aaccgaccca
    20401 gcgaccgttg caccacagat gaaacgccga gttaacgcca tcaaaaataa ttcgcgtctg
    20461 gccttcctgt agccagcttt catcaacatt aaatgtgagc gagtaacaac ccgtcggatt
    20521 ctccgtggga acaaacggcg gattgaccgt aatgggatag gtcacgttgg tgtagatggg
    20581 cgcatcgtaa ccgtgcatct gccagtttga ggggacgacg acagtatcgg cctcaggaag
    20641 atcgcactcc agccagcttt ccggcaccgc ttctggtgcc ggaaaccagg caaagcgcca
    20701 ttcgccattc aggctgcgca actgttggga agggcgatcg gtgcgggctt cttcgctatt
    20761 acgccagctg gcgaaagggg gatgtgccgc aaggcgatta agttgtgtaa cgccagggtt
    20821 ttcccagtca cgacgttgta aaacgacggc cagtgaatcc gtaatcatgg tcatagctgt
    20881 ttcctgtgtg aaattgttat ccgctcacaa tttcacacaa catacgagcc ggaagcataa
    20941 agtgtaaagc ctggggtgcc taatgagtga gctaactcac attaattgcg ttgcgctcac
    21001 tgcccgcttt ccagtcggga aacctgtcgt gccagctgca ttaatgaatc ggccaacgcg
    21061 cggggagagg cggtttgcgt attgggcgcc agggtggttt ttcttttcac cagtgagacg
    21121 ggcaacagct gattgccctt caccgcctgg ccctgagaga gttgcagcaa gcggtccacg
    21181 ctggtttgcc ccagcaggcg aaaatcctgt ttgatggtgg ttaacggcgg gatataacat
    21241 gagctgtctt cggtatcgtc gtatcccact accgagatat ccgcaccaac gcgcagcccg
    21301 gactcggtaa tggcgcgcat tgcgcccagc gccatctgat cgttggcaac cagcatcgca
    21361 gtgggaacga tgccctcatt cagcatttgc atggtttgtt gaaaaccgga catggcactc
    21421 cagtcgcctt cccgttccgc tatcggctga atttgattgc gagtgagata tttatgccag
    21481 ccagccagac gcagacgcgc cgagacagaa cttaatgggc ccgctaacag cgcgatttgc
    21541 tggtgaccca atgcgaccag atgctccacg cccagtcgcg taccgtcttc atgggagaaa
    21601 ataatactgt tgatgggagt ctggtcagag acatcaagaa ataacgccgg aacattagcg
    21661 caggctgctt ccacagcagt ggcatcctgg tcatccagcg gatagttaat gagcagccca
    21721 ctgacgcgtt gcgcgagaag attgtgcacc gccgctttac aggcttcgac gccgcttcgt
    21781 tctaccatcg acaccaccac gctggcaccc agttgatcag cgcgagattt aatcgccgcg
    21841 acaatttgcg acggcgcgtg cagggccaga ctggaggtgg caacgccaat cagcaacgac
    21901 tgtttgcccg ccagttgttg tgccacgcgg ttgggaatgt aattcagctc cgccatcgcc
    21961 gcttccactt tttcccgcgt tttcgcagaa acgtggctgg cctggttcac cacgcgggaa
    22021 acggtctgat aagagacacc ggcatactct gcgacatcgt atagcgttac tggtttcaca
    22081 ttcaccaccc tgaattgact ctcttccgaa cgctatcatg ccataccgcg aaaggttttg
    22141 cgccattcga tggtgtcaac gtaaatgcac gccgcttcgc cgtccggcca ccagaatagc
    22201 ctgcgattca accccttctt cgatctgttt tgctacccgt tgtagcgccg gaagatgctt
    22261 ttccgctgcc tgttcaatgg tcattgcgct cgccatatac accagattca gacagccaat
    22321 cacccgttgt tcactgcgca gcggtacggc gatagaggcg atcttctcct cctgatccca
    22381 gccgcggtag ttctgtccgt aaccctcttt gcgcgcgcgc gccagaatgg cttccagctt
    22441 taacggttcc cgtgccagtt gatagtcatc accggggcgg gaggctaaca tttcgattaa
    22501 ttccttgcgg tcttgttccg ggcaaaaggc cagccaggtc aggcccgagg cggttttcag
    22561 aagcggcaaa cgtcgcccga ccattgcccg gtgaaaggat aagcggctga aacggtgagt
    22621 ggtttcgcgt accaccattg catcaacatc cagcgtggac acatctgtcg gccataccac
    22681 ttcgcgcaac agatcgccca gcagtggggc cgccagtgca gaaatccact gttcgtcacg
    22741 aaatccttcg cttaattgcc gcactttgat ggtcagtcga aaactatcat cggaggggct
    22801 acggcggaca tatccctctt cctgcagcgt ctccagcagt cgccgcacag tggtgcgatg
    22861 caggccgctg agttccgcca gcagcccgac gctggcaccg ccatcaagtt tatttaacat
    22921 atttaataac attagaccgc gggttaagcc gcgcacggtt ttgtattccg tctgctcatt
    22981 gttctgcata ttaattgaca tttctatagt taaaacaacg ttgtgcacct ggtgcacatt
    23041 cgggcatgtt ttgattgtag
//
LOCUS       DAZKHF020000054        22956 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2201S31241
            SAMN43267800-rid25106413.denovo.054, whole genome shotgun sequence.
ACCESSION   DAZKHF020000054 DAZKHF020000000
VERSION     DAZKHF020000054.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267800
            Sequence Read Archive: SRR30307263
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 22956)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22956)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 22956)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350581.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350581.2
            Genome Coverage       :: 151x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:21:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,652
            CDSs (total)                      :: 5,557
            Genes (coding)                    :: 5,320
            CDSs (with protein)               :: 5,320
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 237
            CDSs (without protein)            :: 237
            Pseudo Genes (ambiguous residues) :: 0 of 237
            Pseudo Genes (frameshifted)       :: 102 of 237
            Pseudo Genes (incomplete)         :: 130 of 237
            Pseudo Genes (internal stop)      :: 52 of 237
            Pseudo Genes (multiple problems)  :: 41 of 237
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22956
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267800-rid25106413.denovo.054"
                     /serotype="O 26:H11"
                     /isolate="2201S31241"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-01"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            complement(42..2165)
                     /locus_tag="ACHOSC_RS08440"
                     /old_locus_tag="pgaptmp_001688"
     CDS             complement(42..2165)
                     /locus_tag="ACHOSC_RS08440"
                     /old_locus_tag="pgaptmp_001688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001280406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tailspike protein"
                     /protein_id="HGU4547650.1"
                     /translation="MSDITANVVVSMPSQLFTMSRSFKAVANGKIYIGKIDTDPVNPE
                     NQIQVYVENEDGSHLPVSQPIIINAAGYPVYNGQIAKFVTVQGHSMAVYDAYGAQQFY
                     FPNVLKYDPDQFKGNLLSEDHSLGDSLVMHNPQFVDSTSTILSKKLTIDLRTLTDYGF
                     AEGNTGAQNKAAFQKAIDDATLPTEIVIPEGAFIVDPGITIKNTVTMIRGAGAYQSRI
                     FSTGTAAPIITQQDGVITFCEFRDFGLDGNGYAANGISLTEANHIKIENIDVVNTNNN
                     AILVNGYSIDIIGCRLFQNAGNGINVGGYCNNINIINSRIYGNGAGGVLLTPAYAEGG
                     MSVRVNGNNIEQNKFYGLLAYGVKGLNLDANYWERNGEIGYPYSVPESITVRADIHLI
                     ANNFTLIPDLSKINDTVSIRGNQQTAIGYASALPNQDGFIFTNYAKNLTIENNQLLDA
                     SKVNNLLAMYHNNLSSKVTDRLYLANNTVNSIGYVGSYDPATQNPDTAHLIDIANREL
                     TANYLDRNMLLWTAASGTTGTLIKTQNIYAGNYSFLVTTGDRVWGRTIDLNKSPELKG
                     KFVWFGAWVNDQGSASKLMFIINGAGQTDSTAPLAGNGKWSYVSCGVYIYETDTAINV
                     GIRNYGSGNVLINSPSLCAYGMPSNALQVEKTTFYLSSVPTSGFWDIGERVINSAPAS
                     GQPKAWTCNIPGGPGTYSFLSEGNF"
     gene            2287..2553
                     /locus_tag="ACHOSC_RS08445"
                     /old_locus_tag="pgaptmp_001689"
     CDS             2287..2553
                     /locus_tag="ACHOSC_RS08445"
                     /old_locus_tag="pgaptmp_001689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000287048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Arc family DNA-binding protein"
                     /protein_id="HGU4547651.1"
                     /translation="MYSKYDEAQFHLRLPHELHAKIKQRAKMNNRSLNSEIIAAIEES
                     LAKQSSASVYIDDAERMAEQQSDMVKKMVFETLKTMYSNNKKET"
     gene            2624..3109
                     /locus_tag="ACHOSC_RS08450"
                     /old_locus_tag="pgaptmp_001690"
     CDS             2624..3109
                     /locus_tag="ACHOSC_RS08450"
                     /old_locus_tag="pgaptmp_001690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000749284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4547652.1"
                     /translation="MKKSLLIIPLLLVGCAKVSDYQASCEQRYQKLSDMANCLDASVK
                     NDSRMASAPTPKLYVLAAKMLGKGVDEGKISDAQARLELQNLYVQLQSQEQAQQIAQS
                     QAFQQALLNYQAVNTMQAIEQKARQPVITQPYPTRVDTYTNCNSGFGNTVTCNSSSNI
                     R"
     gene            complement(3124..4968)
                     /locus_tag="ACHOSC_RS08455"
                     /old_locus_tag="pgaptmp_001691"
     CDS             complement(3124..4968)
                     /locus_tag="ACHOSC_RS08455"
                     /old_locus_tag="pgaptmp_001691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000868968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA transfer protein"
                     /protein_id="HGU4547653.1"
                     /translation="MKVTANGKTFTFPDGTSTEDIGTAIDEYFAGQAVQQQTVNQANN
                     APTRDEPSLMQQAGDWLTGGQSAGQIAEQAGRGLVNIPFDVLQGGASLINAISQGLGG
                     PKVLDDVYRPVDKPTDPYAQAGETIGGYLVPGVGTAGSMAIGSLAEAANQKGDFAQNA
                     AKNAGVNLAAQGVLSAAAKGIGRGITAIKGDIAPEVAKKIATSESMGVTPMTSDVIPP
                     KNAFTRGLTQDAEGALLGTGSKRAEQYATRSKLVSNYFDRFGEYNPDDVVKSLTTTLR
                     GRKDAAGAVINDVTNKMGNAAVDTTNTMNALNTAIARQERLGTSANQSLLTSLRNLRE
                     ELANPATDLDVTFDLLRQHRTAFRSNVQGDAMVFPNQAKAATNMVENAMSKDLRNAVA
                     KNLGASDAAKYLKANSDYANVYNKVLNKNIANKLNKASSEASPELINTVVLSRKPSDV
                     KRIWSALDDKGKDAMRAAYVSKIAEKAGDSPAKFITEVNKLKSQSGGEIYNTIFSGKH
                     MKELDSLHEVLQKTARSDTANVVTQTGQSQANRIRTIGATATLGVSMGLEAGFGAMMR
                     LYESKAARNALLRLANTKAGTPAYERALNNAANAIRPILSSQITAEQQ"
     gene            complement(4968..6374)
                     /locus_tag="ACHOSC_RS08460"
                     /old_locus_tag="pgaptmp_001692"
     CDS             complement(4968..6374)
                     /locus_tag="ACHOSC_RS08460"
                     /old_locus_tag="pgaptmp_001692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000246937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage DNA ejection protein"
                     /protein_id="HGU4547654.1"
                     /translation="MATWQGTNGGLLAGIGGVNSNAPSVNDIGNTLQLIRQNNDIDRS
                     GANNVGLTALQGLSGIAGVFQQEKQAQRQKEFQQAYANAYASGDRGALRQLATQYPDQ
                     IESVRKGMGFIDEDQRNSIGTLAAGARLASSSPEAMQSWLQNNAKELTRVGVDPNSVA
                     QMYQQNPSGFGEFVDHLGMAALGPIDYFNVQDKMAGREIDRGRLAETIRSNQAGEALQ
                     ARGQNLSYQSAMTGHNIAAQRLALDQQEFGFKMQQAQEKAQQLISEAPKLSVNMEKGI
                     ETAVNNATASSNSANSMSALAQQFRAEKPTTGLFGNAQNMFAKLTGSDTTLRDLRIRQ
                     NALVNSQVLKFLPPGPATDKDVEIVRQGAPTDMDNPETVARWLDAMANLERRNAQFNE
                     FKAEWMSANGNPGQSRNGGQILGLDVKKGESLGSAVKRYMSMNTDAAPAQDSTPSGEP
                     RNQVGSYTSKSGIQFTVE"
     gene            complement(6384..7076)
                     /locus_tag="ACHOSC_RS08465"
                     /old_locus_tag="pgaptmp_001693"
     CDS             complement(6384..7076)
                     /locus_tag="ACHOSC_RS08465"
                     /old_locus_tag="pgaptmp_001693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA transfer protein"
                     /protein_id="HGU4547655.1"
                     /translation="MLYAFKLGRKLRGEEPYCPEKGGKGGSSDKSAKYAAEAQKYAAD
                     LQNQQWQTIMKNLAPFTPLAEQYVNQLQNLSSLEGQGQALNQYYNSQQYKDLAGQARY
                     QSLAAAEATGGLGSTATSNQLATIAPTLGQSWLSNQMSNYNNLANVGLGALQGQANAG
                     QTYANNMSSIAQQSAALAAANANKPSSLQTAISGGTSGAIAGAGLASLLGTSTPWGAG
                     IGAGIGLLGSLF"
     gene            complement(7079..7534)
                     /locus_tag="ACHOSC_RS08470"
                     /old_locus_tag="pgaptmp_001694"
     CDS             complement(7079..7534)
                     /locus_tag="ACHOSC_RS08470"
                     /old_locus_tag="pgaptmp_001694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706178.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2824 family protein"
                     /protein_id="HGU4547656.1"
                     /translation="MIQFKPTRNIDLIEAVGNHPDIIAGSNNGDGYDYKPECRYFEVN
                     VYGQFGGIVYYQEIQPLTFDCHAMYLPEVRGFSKEIGLAFWRYILTNTTVQCVTSFAA
                     RKFRHGQMYCAMIGLKRVGTIKKYFKGVDDVTFYSATREELIDFLNHGR"
     gene            complement(7534..8382)
                     /locus_tag="ACHOSC_RS08475"
                     /old_locus_tag="pgaptmp_001695"
     CDS             complement(7534..8382)
                     /locus_tag="ACHOSC_RS08475"
                     /old_locus_tag="pgaptmp_001695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tail needle knob protein"
                     /protein_id="HGU4547657.1"
                     /translation="MADSNLNVPVIIQATRLDTSVLPRNIFSQSYLLYVIAQGTDVGN
                     VANKANEAGQGAYDAQVRNDEQDVILADHEQRISAAEATLVNHEERISQAESTLQEHE
                     TRIAQNESDIASLDTRVQSLESQVSDHESRIDALEYATTRKKSEVVYSGVSVTIPTAP
                     TNLVSLLKTLTPSSGSLAPFFDTVNNKMVVFNENKTLFFKLSIVGTWPSGTANRSMQL
                     TFSGSVPDTLVSSRNSATTTDNILLATFFSVDKDGFLATNGSTLTIQSNGAAFTATTI
                     KIIAEQ"
     gene            complement(8382..9800)
                     /locus_tag="ACHOSC_RS08480"
                     /old_locus_tag="pgaptmp_001696"
     CDS             complement(8382..9800)
                     /locus_tag="ACHOSC_RS08480"
                     /old_locus_tag="pgaptmp_001696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="packaged DNA stabilization protein gp10"
                     /protein_id="HGU4547658.1"
                     /translation="MPIQQLPLMKGVGKDFRNADYIDYLPVNMLATPKEILNSSGYLR
                     SFPGIAKRSDVNGVSRGVEYNMAQNAVYRVCGGKLYKGESEVGDVAGSGRVSMAHGRT
                     SQAVGVNGQLVEYRYDGTVKTVSNWPADSGFMQYELGSVRDITRLRGRYAWSKDGTDS
                     WFITDPEDESHPDRYSAQYRAESQPDGIIGIGTWRDFIVCFGSSTIEYFSLTGATTVG
                     AALYVAQPSLMVQKGIAGTYCKTPFADSYAFISNPATGAPSVYIIGSGQVSPIASASI
                     EKILRSYTADELADGVMESLRFDAHELLIIHLPRHVLVYDASSSANGPQWCVLKTGLY
                     DDVYRAIDFIYEGNQITCGDKLESVTGKLQFDISSQYGLQQEHLLFTPLFKADNARCF
                     DLEVESSTGVAQYADRLFLSATTDGINYGREQMIEQNEPFVYDKRVLWKRVGRIRKNV
                     GFKLRVITKSPVTLSGAQIRIE"
     gene            complement(9809..10291)
                     /locus_tag="ACHOSC_RS08485"
                     /old_locus_tag="pgaptmp_001697"
     CDS             complement(9809..10291)
                     /locus_tag="ACHOSC_RS08485"
                     /old_locus_tag="pgaptmp_001697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000246747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="packaged DNA stabilization gp4 family protein"
                     /protein_id="HGU4547659.1"
                     /translation="MATVLTKGEIVLFALRKFAIASNASLTDVEPQSIEDGVNDLEDM
                     MSEWMINPGDIGYAFATGDEQPLPDDESGLPRKYKHAVGYQLLLRMLSDYSLEPTPQV
                     LSNAQRSYDALMTDTLVVPSMRRRGDFPVGQGNKYDVFTSDRYYPGDLPLIDGDIPNA
                     "
     gene            complement(10266..10451)
                     /locus_tag="ACHOSC_RS08490"
                     /old_locus_tag="pgaptmp_001698"
     CDS             complement(10266..10451)
                     /locus_tag="ACHOSC_RS08490"
                     /old_locus_tag="pgaptmp_001698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000375637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4547660.1"
                     /translation="MDRMSVFLAADNESGHVQAVIAEKDFQFFERLGFVASVDELKPT
                     SKRGRKAADNGNSTDKG"
     gene            complement(10494..11765)
                     /locus_tag="ACHOSC_RS08495"
                     /old_locus_tag="pgaptmp_001699"
     CDS             complement(10494..11765)
                     /locus_tag="ACHOSC_RS08495"
                     /old_locus_tag="pgaptmp_001699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015973865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P22 phage major capsid protein family protein"
                     /protein_id="HGU4547661.1"
                     /translation="MPNNLDSNVSQIVLKKFLPGFMSDLVLAKTVDRQLLAGEINSST
                     GDSVSFKRPHQFSSLRTPTGDISGQNKNNLISGKATGRVGNYITVAVEYQQLEEAIKL
                     NQLEEILAPVRQRIVTDLETELAHFMMNNGALSLGSPNTPITKWSDVAQTASFLKDLG
                     VNEGENYAVMDPWSAQRLADAQTGLHASDQLVRTAWENAQIPTNFGGIRALMSNGLAS
                     RTQGAFGGTLTVKTQPTVTYNAVKDSYQFTVTLTGATASVTGFLKAGDQVKFTNTYWL
                     QQQTKQALYNGATPISFTATVTADANSDSSGDVTVTLSGVPIYDTTNPQYNSVSRQVE
                     AGDAVSVVGTASQTMKPNLFYNKFFCGLGSIPLPKLHSIDSAVATYEGFSIRVHKYAD
                     GDANVQKMRFDLLPAYVCFNPHMGGQFFGNP"
     gene            complement(11777..12661)
                     /locus_tag="ACHOSC_RS08500"
                     /old_locus_tag="pgaptmp_001700"
     CDS             complement(11777..12661)
                     /locus_tag="ACHOSC_RS08500"
                     /old_locus_tag="pgaptmp_001700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000426735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage capsid protein"
                     /protein_id="HGU4547662.1"
                     /translation="MENELIIDGQVIDLSETQENAEETIIQTESQPENESQDDNGKEM
                     ATDPEKTEETPEDYALRIGDEEIQLNADDNDHIDGQPAPQWVKDLRKGFKETQKENRE
                     LRRQLEEALAKHAEHQQPQPDAIPPKPTLESCDYDEQAFEQALTDWHEKKGRVEQQQQ
                     QKLRQQQEYQQRFQQRVEAHKQRAAKLPVKDYQEMEAIVLSELPPIQQEIIIHCADEG
                     SELLAYGLGKSQQLRQRVAAETDPIRAAFLLGQISKQVSLAPKPKKAIKPEPEVRGGG
                     ADAKQDEFNKLCPGAKIE"
     gene            complement(12675..14801)
                     /locus_tag="ACHOSC_RS08505"
                     /old_locus_tag="pgaptmp_001701"
     CDS             complement(12675..14801)
                     /locus_tag="ACHOSC_RS08505"
                     /old_locus_tag="pgaptmp_001701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015966866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="portal protein"
                     /protein_id="HGU4547663.1"
                     /translation="MAETLEKKHERIMLRFDRAYSPQQEVREKCIEATRFARVPGGQW
                     EGATAAGTKLDEQFEKYPKFEINKVATELNRIIAEYRNNRITVKFRPGDREASEELAN
                     KLNGLFRADYEETDGGEACDNAFDDAATGGFGCFRLTSMLVNEYDPMDDRQRIAIEPI
                     YDPSRSVWFDPDAKKYDKSDALWAFCMYSLSPEKYEAEYRKKPPASLDVTSMTSWEYD
                     WFDEDVIYIAKYYEVRKESVVVISYRHPITGEIATYDSDQVEDIEDELAIAGFQEVAR
                     RSVKRRRVYVSVVDGDGFLEKPRRIPGEHIPLIPVYGKRWFIDDIERVEGHIAKAMDP
                     QRLYNLQVSMLADTAAQDPGQIPIVGMEQIRGLEKHWEARNKKRPAFLPLREVRDKSG
                     NIIAGATPAGYTQPAVMNQALAALLQQTSADIQEVTGGSQAMQQMPSNIAQETVNNLM
                     NRADMASFIYLDNMAKSLKRAGEVWLSMAREVYGSEREVRIVNEDGSDDIAVLSAQVV
                     DRQTGAVVALNDLSVGRYDVTVDVGPSYTARRDATVSVLTNVLSSMLPTDPMRPAIQG
                     IILDNIDGEGLDDFKEYNRNQLLISGIAKPRNEKEQQIVQQAQMAAQSQPNPEMVLAQ
                     AQMVAAQAEAQKATNETAQTQIKAFTAQQDAMESQANTVYKLAQARNIDDKAVMEAIR
                     LLKDVAESQQQQFQSPPQSPADLMPS"
     gene            complement(14804..16216)
                     /locus_tag="ACHOSC_RS08510"
                     /old_locus_tag="pgaptmp_001702"
     CDS             complement(14804..16216)
                     /locus_tag="ACHOSC_RS08510"
                     /old_locus_tag="pgaptmp_001702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015966865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PBSX family phage terminase large subunit"
                     /protein_id="HGU4547664.1"
                     /translation="MTSINPIFEPFIEAHRYKVAKGGRGSGKSWAIARLLVEAARRQP
                     VRILCARELQNSISDSVIRLLEDTIEREGYSAEFEIQRSMIRHLGTNAEFMFYGIKNN
                     PTKIKSLEGIDICWVEEAEAVTKESWDILIPTIRKPFSEIWVSFNPKNILDDTYQRFV
                     VNPPDDICLLTVNYTDNPHFPEVLRLEMEECKRRNPTLYRHIWLGEPVSASDMAIIKR
                     EWLEAATDAHTKLGWKAKGAVVSAHDPSDTGPDAKGYASRHGSVVKRIAEGLLMDINE
                     GADWATSLAIEDGADHYLWDGDGVGAGLRRQTTEAFSGKKITATMFKGSESPFDEDGP
                     YQAGAWADEVVQGDNVRTIGDVFRNKRAQFYYALADRLYLTYRAVVHGEYADPDDMLS
                     FDKEAIGEKMLEKLFAELTQIQRKFNNNGKLELMTKVEMKQKLGIPSPNLADALMMCM
                     HCPALVREETEIYVPSSSGW"
     gene            complement(16213..16638)
                     /locus_tag="ACHOSC_RS08515"
                     /old_locus_tag="pgaptmp_001703"
     CDS             complement(16213..16638)
                     /locus_tag="ACHOSC_RS08515"
                     /old_locus_tag="pgaptmp_001703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000179910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ubiquitin carboxyl-hydrolase"
                     /protein_id="HGU4547665.1"
                     /translation="MATEKKKGGRPSDYMPEVANDICALLSSGESLRKVCERPGMPSK
                     TSVFRWLAEHQEFRDQYAKATETRADSIFEEIFEIADDVIPDAAEVAKARLRVDTRKW
                     ALARMNPRKYGDKVTNELVGKDGGAIQIETSPMSTLFGK"
     gene            complement(16718..16960)
                     /locus_tag="ACHOSC_RS08520"
                     /old_locus_tag="pgaptmp_001704"
     CDS             complement(16718..16960)
                     /locus_tag="ACHOSC_RS08520"
                     /old_locus_tag="pgaptmp_001704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001561773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2560 family protein"
                     /protein_id="HGU4547666.1"
                     /translation="MAEIIPMTEEQKFQLEIYKLVMNQNAAAEEAFQFIGTDELKLEL
                     FKIHFQSGGANSDITTRTIEAVRKSKEALDLFTTGA"
     gene            complement(17064..17435)
                     /locus_tag="ACHOSC_RS08525"
                     /old_locus_tag="pgaptmp_001705"
     CDS             complement(17064..17435)
                     /locus_tag="ACHOSC_RS08525"
                     /old_locus_tag="pgaptmp_001705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001595512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gp49 family protein"
                     /protein_id="HGU4547667.1"
                     /translation="MNDQQIEKEIVEKGKTAPRVTPDHIEGIIAQEAYFTAEDGAFGK
                     AIKAKHTGGEVNYQSHESLSLLTFCVLVLRNGFTVTGESACASQENFDAEIGRKIARQ
                     NAVNKIWMLEGYLLKQKLSEQ"
     gene            complement(17719..18237)
                     /locus_tag="ACHOSC_RS08530"
                     /old_locus_tag="pgaptmp_001706"
     CDS             complement(17719..18237)
                     /locus_tag="ACHOSC_RS08530"
                     /old_locus_tag="pgaptmp_001706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001016387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rha family transcriptional regulator"
                     /protein_id="HGU4547668.1"
                     /translation="MNHQLANLDFRDMVVVSGDRVITTSRKVAAYFDKQHHHIIQKIE
                     KLDCSDEFLTSNFSRVTYEHKGNQYVEYEISKDGAMYIIMSFTGKKAAAIKEAFIKAF
                     NWMRDRLMEMAHSYQREHNELMLEFMKEKDVASMSGRLLNRWGRIKKPQLIARIKRLE
                     QQAQISIPGLPK"
     gene            complement(18443..18880)
                     /locus_tag="ACHOSC_RS08535"
                     /old_locus_tag="pgaptmp_001707"
     CDS             complement(18443..18880)
                     /locus_tag="ACHOSC_RS08535"
                     /old_locus_tag="pgaptmp_001707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009453323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysis protein"
                     /protein_id="HGU4547669.1"
                     /translation="MSRVTAIISALVICIIVCLSWAVNHYRDNAMTYKEQRDKATSII
                     ADMQKRQRDVAELDARYTKELADANATIESLRADVSAGRKRLQVAATCAKSTTGASSM
                     GDGESPRLTADAELNYYRLRSGIDRITAQVNYLQEYIRTQCLK"
     gene            complement(18877..19353)
                     /locus_tag="ACHOSC_RS08540"
                     /old_locus_tag="pgaptmp_001708"
     CDS             complement(18877..19353)
                     /locus_tag="ACHOSC_RS08540"
                     /old_locus_tag="pgaptmp_001708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003796 - lysozyme activity [Evidence
                     IEA];
                     GO_process: GO:0009253 - peptidoglycan catabolic process
                     [Evidence IEA];
                     GO_process: GO:0016998 - cell wall macromolecule catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family protein"
                     /protein_id="HGU4547670.1"
                     /translation="MVEINNQRKAFLDMLAWSEGTDNGRQKTRNHGYDVIVGGELFTD
                     YSDHPRKLVTLNPKLKSTAAGRYQLLSRWWDAYRKQLGLKDFSPKSQDAVALQQIKER
                     GALPMIDRGDIRQAIDRCSNIWASLPGAGYGQFEHKADSLIAKFKEAGGTVREIEV"
     gene            complement(19337..19660)
                     /locus_tag="ACHOSC_RS08545"
                     /old_locus_tag="pgaptmp_001709"
     CDS             complement(19337..19660)
                     /locus_tag="ACHOSC_RS08545"
                     /old_locus_tag="pgaptmp_001709"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015966862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0019835 - cytolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin, lambda family"
                     /protein_id="HGU4547671.1"
                     /translation="MKMPEKHDLLAAILAAKEQGIGAILAFAMAYLRGRYNGGAFTKT
                     VIDATMCAIIAWFIRDLLDFAGLSSNLAYITSVFIGYIGTDSIGSLIKRFAAKKAGVE
                     DGGNQ"
     gene            complement(20733..21356)
                     /locus_tag="ACHOSC_RS08550"
                     /old_locus_tag="pgaptmp_001710"
     CDS             complement(20733..21356)
                     /locus_tag="ACHOSC_RS08550"
                     /old_locus_tag="pgaptmp_001710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="antitermination protein"
                     /protein_id="HGU4547672.1"
                     /translation="MRLESVAKFHSPKSPMMSDSPRATASDSLSGTDVMAAMGMAQSQ
                     AGFGMAAFCGKHELSQNDKQKAINYLMQFAHKVSGKYRGVAKLEGNTKAKVLQVLATF
                     AYADYCRSAATPGARCRDCHGTGRAVDIAKTEQWGRVVEKECGRCKGVGYSRMPASAA
                     YRAVTMLIPNLTQPTWSRTVKPLYDALVVQCHKEESIADNILNAVTR"
     gene            complement(21353..22018)
                     /locus_tag="ACHOSC_RS08555"
                     /old_locus_tag="pgaptmp_001711"
     CDS             complement(21353..22018)
                     /locus_tag="ACHOSC_RS08555"
                     /old_locus_tag="pgaptmp_001711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine protein phosphatase"
                     /protein_id="HGU4547673.1"
                     /translation="MRYYERIDGSKYRNIWVVGDLHGCYTNLMKKLETIGFDTKKDLL
                     ISVGDLVDRGTENVECLELITFPWFRAVRGNHEQMMIDGLSERGNVNHWLLNGGGWFF
                     NLDYDKEILAKALAHKAGELPLIIELVSKDKKYVICHADYPCDEYEFGKPVDHQQLIW
                     NRERISNSQDGIVKEIKGADMFIFGHTPAVKPLKFANQMYIDTGAVFCGNLTLIHVQG
                     EGA"
     gene            complement(21996..22202)
                     /locus_tag="ACHOSC_RS08560"
                     /old_locus_tag="pgaptmp_001712"
     CDS             complement(21996..22202)
                     /locus_tag="ACHOSC_RS08560"
                     /old_locus_tag="pgaptmp_001712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001601990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage NinH family protein"
                     /protein_id="HGU4547674.1"
                     /translation="MTFSVKTIPDMLVEAYGNQTEVARRLKCSRGTVRKYVDDKDGKM
                     HAIVNDVLMVHRGWSERDALLRKN"
     gene            complement(22199..22803)
                     /locus_tag="ACHOSC_RS08565"
                     /old_locus_tag="pgaptmp_001713"
                     /pseudo
     CDS             complement(22199..22803)
                     /locus_tag="ACHOSC_RS08565"
                     /old_locus_tag="pgaptmp_001713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309228.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="recombination protein NinG"
     gene            complement(22796..>22956)
                     /locus_tag="ACHOSC_RS08570"
                     /old_locus_tag="pgaptmp_001714"
     CDS             complement(22796..>22956)
                     /locus_tag="ACHOSC_RS08570"
                     /old_locus_tag="pgaptmp_001714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="protein NinF"
                     /protein_id="HGU4547675.1"
                     /translation="SLQYQKESVERALTCANCGQKLHVLEVHVCEHCCAELMSDPNSS
                     MYEEEYDG"
BASE COUNT         5443 a         5723 c         5227 g         6563 t
ORIGIN      
        1 aacataccag atcaaagtaa tcatttcaat acataggtct tttaaaagtt tccctctgat
       61 aagaaagaat aggttcctgg cccacctgga atattgcatg tccatgcctt tggttgtcct
      121 gaagcaggtg cgctattgat aacacgctca cctatatccc agaaaccgga tgtaggtact
      181 gacgaaagat aaaatgttgt tttttcaact tgcaacgcgt ttgatggcat tccatatgca
      241 catagagaag gtgagtttat gagtacattt cctgaaccat aatttcttat accaacattt
      301 atagctgtat cagtttcgta tatatacaca ccacaactaa catagctcca tttcccatta
      361 ccagctaaag gtgctgtaga gtctgtttgt cctgcaccat taattataaa cataagtttt
      421 gacgctgatc cctggtcatt gacccacgca ccaaaccata caaatttccc ttttagttct
      481 ggtgatttgt tgaggtctat cgttcgtccc caaactctat ctcctgtcgt aactaagaat
      541 gaataattac cagcatagat gttttgagtt tttatcaatg ttcctgtagt tccagatgcc
      601 gcagtccaga gcaacatatt tcgatcaaga tagttagcag ttaactctct gttcgcaata
      661 tctattaaat gcgcagtgtc agggttttgt gtcgccgggt cataactacc aacgtatccg
      721 atggagttca ctgtgttgtt ggcaagatat agcctgtcag taactttgga cgaaagatta
      781 ttgtggtaca ttgctaataa attattcact ttcgatgcgt ctaataactg attattttca
      841 atcgtcaggt ttttcgcata gttcgtgaaa ataaatccgt cttggttagg tagcgcgctg
      901 gcgtagccaa tcgcggtctg ctgattcccg cgaatagata cggtatcatt aatttttgaa
      961 aggtcaggaa ttaatgtaaa attgttcgcg ataaggtgga tatccgcacg tactgtaata
     1021 gactcaggta cactatacgg gtaaccaatc tcaccgttgc gttcccaata gttagcatca
     1081 aggttaaggc ctttaacccc gtaagccaaa agtccgtaga acttattttg ctctatattg
     1141 ttgccattaa ctctgacgct cataccgccc tcagcgtaag ctggcgtaag taaaactcca
     1201 ccggctccgt taccatagat acggctgttg attatattaa tattattgca gtaaccacca
     1261 acattaatac cgttaccggc gttttgaaac aaccggcacc caatgatgtc aatggagtaa
     1321 ccgttaacta atattgcgtt gttattagta ttaaccacgt caatattttc tattttaatg
     1381 tggttggctt cagtaagact gatgccgtta gctgcgtagc catttccgtc aagaccaaag
     1441 tccctgaact cgcagaacgt aattacccca tcttgttgcg tgattatcgg tgccgctgta
     1501 cccgtagaga aaattctgga ctgatatgcg ccagcacctc gtatcatggt gacagtattc
     1561 ttaatcgtaa taccagggtc aacaatgaac gccccctctg gtataacaat ctcagttggc
     1621 aacgtagcat catcaatagc cttctgaaat gcagctttgt tctgagcacc ggtgtttccc
     1681 tcagcaaacc cgtagtctgt aagcgtcctc aggtcgatag ttagcttttt tgagagaatg
     1741 gtcgatgtgc tatcgacaaa ctgtgggtta tgcatgacta gagagtcgcc aagtgagtga
     1801 tcttcgctca ggagattgcc cttaaactga tctggatcat actttagcac attcggaaaa
     1861 tagaactgct gcgcaccgta tgcatcataa acagccatag aatggccttg cacagttacg
     1921 aacttggcaa tctgtccgtt atatacaggg taaccagcag cgttaatgat gattggttgc
     1981 gaaacaggaa ggtgagaacc gtcttcgttt tccacataaa cctgaatctg gttttctgga
     2041 tttaccgggt cagtgtcaat tttaccgata taaatttttc cattggctac ggctttaaaa
     2101 gagcgagaca tagtgaagag ttgcgaaggc atgctgacca caacatttgc ggtgatatct
     2161 gacatttcat tgctccagaa gaatgatatg atgcaaccat gatgtgattg cataccgaaa
     2221 tggtactatt gagtatttat ccagtaggtt acgatgccat tccacccaac tggtgaggca
     2281 tcaaggatgt acagcaaata cgacgaggcg cagtttcact tgagacttcc gcatgaactc
     2341 cacgcgaaaa ttaaacagcg cgcgaagatg aataacaggt cgctgaactc agagataatt
     2401 gcagcgattg aagaatcgtt ggctaaacaa agctctgcat ccgtttacat tgatgatgca
     2461 gagcgtatgg cagaacaaca atctgatatg gttaagaaaa tggtttttga aacgcttaag
     2521 accatgtata gcaataataa aaaggaaaca tagaaatcta gtttccggct aaaatggcat
     2581 tgccttcatg atatcctgtg aaaaactaag gagagttaac catatgaaaa aatcactgtt
     2641 aattatcccg cttctgctgg ttggatgcgc aaaagtaagt gactatcaag caagttgcga
     2701 acaacgctat caaaagctta gcgatatggc taattgcctt gatgccagtg tgaagaacga
     2761 ctcacgcatg gcatcagcac caacacctaa gctgtatgtc cttgctgcaa agatgctcgg
     2821 gaaaggtgtc gatgaaggca agataagtga cgcacaggca agacttgagc ttcagaatct
     2881 ttatgttcaa ttacaaagcc aagaacaagc ccaacaaata gcacaaagcc aagcattcca
     2941 gcaggcttta ttgaattatc aggctgtaaa cacaatgcaa gcgatcgagc aaaaagcgcg
     3001 ccagcctgtt ataactcaac cttacccaac acgcgttgac acatatacaa actgcaattc
     3061 aggatttgga aacacggtaa catgcaacag tagcagtaat atcaggtagt tacatcttat
     3121 cttttattgc tgttctgctg taatttggct tgagagtata gggcgtatcg catttgcagc
     3181 attatttagc gctctttcat aggctggtgt tcctgctttg gtgtttgcca aacgtaagag
     3241 agcattcctt gctgctttgg actcatacaa gcgcatcatt gcaccgaaac cagcctcaag
     3301 ccccattgat acgccaagag tcgcagttgc gccaatcgtc cttatcctgt tggcttgcga
     3361 ttgcccagtc tgagttacta catttgcggt gtctgacctt gctgttttct gtagaacttc
     3421 atgaagagaa tcaagctctt tcatgtgctt tccagaaaaa atagtgttgt aaatttcacc
     3481 acctgactga gatttcagct tattaacttc agtgatgaac ttggctggag agtcaccggc
     3541 cttttccgct attttgctga cgtaagctgc acgcatagca tctttccctt tatcatccaa
     3601 tgcgctccag attcgtttca cgtcagatgg ttttctgctt aatacaacag tatttataag
     3661 ttcaggactg gcttcactgc ttgccttgtt gagcttgtta gcaatgtttt tattaagcac
     3721 cttattataa acgtttgcat aatcggaatt tgctttaagg tattttgctg cgtctgacgc
     3781 accgaggttt tttgcaactg cgttacgaag gtcttttgac attgcattct ctaccatatt
     3841 ggtagctgct tttgcctggt tggggaagac catagcatct ccctgaacat tagatctaaa
     3901 tgctgttctg tgctgacgca agagatcaaa cgtaacatcc aaatcagttg cagggtttgc
     3961 taattcttca cgcaggttac gcaaggatgt aagcaggctt tgattggctg aagtcccaag
     4021 ccgttcctgt cttgcgatcg ctgtattcag agcattcatg gtatttgtag tatcaactgc
     4081 ggcattaccc attttattgg tgacgtcatt gataacagcg ccagcagcat ccttccgccc
     4141 ccttaacgtg gtggtcagag atttcaccac atcatcaggg ttgtactcac caaaacggtc
     4201 aaaataattg cttaccagct tactacgcgt tgcatattgc tctgctcgct ttgagcccgt
     4261 cccgagcaaa gccccctcgg catcctgagt aaggccgcga gtgaaagcat ttttcggcgg
     4321 gataacatca gatgtcattg gtgtcacgcc catcgattct gatgtggcaa ttttcttcgc
     4381 cacttctggc gcaatatcac cttttatagc cgttattcca cgccctattc cctttgctgc
     4441 tgcggaaaga acaccctgag cggcaaggtt aactccggca tttttagctg cattttgtgc
     4501 gaaatcgcct ttctgatttg cggcctctgc cagcgatcca atagccatgc ttcctgccgt
     4561 tccaactcct ggaactaaat acccaccaat tgtttcaccg gcttgtgcgt atgggtctgt
     4621 cggtttgtct actggacgat aaacatcatc caaaactttt ggcccaccaa gcccctgact
     4681 gattgcatta atcagacttg cgccaccctg caatacgtca aatggtatgt ttaccagacc
     4741 acgaccagcc tgttctgcaa tttgccctgc actttgacca ccagtgagcc aatcgccagc
     4801 ttgttgcatc aatgatggtt cgtcccgtgt tggtgcatta ttggcctgat taactgtttg
     4861 ttgctgaaca gcctgaccag caaaatactc atcaatggcg gtgccaatat cttcggtgct
     4921 cgtaccatca ggaaaggtaa atgtcttacc gtttgcagtt actttcatca ttccaccgta
     4981 aattgaatgc ctgattttga ggtatatgat ccaacctgat tccgtggttc tcctgaaggt
     5041 gtcgaatctt gtgctggcgc tgcgtcagta ttcattgaca tataccgctt aacggcactc
     5101 cccaatgatt cacctttttt aacatccaac cccaatatct gaccgccatt acgcgattgt
     5161 ccagggttgc cattcgcgct catccactcg gctttaaact cattaaactg cgcgtttcgt
     5221 cgctcaaggt ttgccattgc atcaagccat cttgcgaccg tctcagggtt atccatgtca
     5281 gttggcgcac cctgtcgaac gatctcaacg tctttatccg ttgctgggcc gggaggtagg
     5341 aatttaagaa cctgactgtt aacaagggca ttttggcgaa tgcgcaaatc acgcaatgtt
     5401 gtatcgcttc cggtaagttt tgcgaacatg ttctgtgcgt taccgaacaa acctgtcgtt
     5461 ggtttttctg ctctgaactg ttgagcaagc gcactcatag aattggctga gtttgatgat
     5521 gctgtagcat tgtttacagc cgtctcgatg cctttttcca tgtttactga cagcttaggt
     5581 gcttcgctaa tcaactgctg agccttttcc tgcgcttgct gcatcttaaa cccgaactct
     5641 tgctgatcca gagccaagcg ttgtgctgcg atattgtgcc cagtcattgc tgactgatag
     5701 gaaaggtttt gccctctcgc ctgaagtgct tctccagcct gattgctgcg gattgtctct
     5761 gccagtctgc ctcggtcaat ttcacgacca gccatcttgt cctgaacatt gaagtaatca
     5821 attggaccaa gagcagccat tccaaggtga tcaacaaact caccaaatcc tgaagggttc
     5881 tgctgataca tctgagcaac gctgttaggg tcaacaccga cgcgagtcag ttccttggcg
     5941 ttgttttgca gccatgattg cattgcttct ggagacgatg acgcaaggcg tgcgccagcc
     6001 gctaaggtgc cgatagaatt acgctggtct tcatcaatga atcccatgcc tttacgaacg
     6061 gattcaatct ggtctggata ttgagtagcc aactgacgca aagcaccgcg atcaccagac
     6121 gcataagcat tagcgtacgc ctgctgaaat tctttctgcc gctgagcctg cttttcctgc
     6181 tgaaacaccc ctgcaatacc tgaaaggcct tgcaaagcag tcagcccaac attgttagcg
     6241 cctgaacgat caatatcatt gttctgcctg ataagctgaa gcgtattgcc gatgtcattt
     6301 acgctcggag cgtttgagtt gacgccgccg ataccagcca acaatccgcc gtttgttcct
     6361 tgccaagtag ccatgattac cccttaaaac aacgagccaa gcaatccgat accagcacca
     6421 atgccagcgc cccaaggcgt tgatgttccc aaaaggctgg caagacctgc accggcaatc
     6481 gcaccagatg tgccgccgct aattgcagtc tgaagacttg atggtttatt ggcattagca
     6541 gcggcaagtg ctgcgctttg ctgtgcaatg ctgctcatgt tgttggcgta cgtctgcccg
     6601 gcgtttgcct gaccttgcag tgcaccaagc ccaacgtttg ccagattgtt gtaattgctc
     6661 atctgatttg ataaccaaga ctgaccgagt gtcggcgcga tcgtagccag ttgattgctt
     6721 gtggctgtcg aaccaagtcc acccgtcgcc tccgcagcag caagactctg gtaacgagcc
     6781 tgacctgcaa ggtctttata ctgctgagag ttgtaatact gattaagtgc ctgcccctga
     6841 ccttctaaac tggaaagatt ctgaagctgg ttaacatact gctccgcaag cggcgtgaac
     6901 ggagcaaggt ttttcatgat cgtctgccac tgctgatttt gcaggtctgc ggcatacttc
     6961 tgagcttctg ctgcatactt tgcgctttta tcagagctgc cacctttccc gcctttttca
     7021 gggcaataag gttcctcgcc gcgcagtttt ctgcccagct taaatgcata taacatagct
     7081 atctcccgtg attcaggaag tcgattagtt cttcgcgtgt ggcgctgtaa aacgtcacgt
     7141 catccacgcc tttgaagtat ttcttgatgg ttcctacacg cttaaggcca atcattgcgc
     7201 agtacatctg accgtggcgg aatttgcgcg cagcgaacga tgtgacgcac tgaacggtgg
     7261 tgttagtcag aatgtatcgc cagaacgcca gcccgatttc cttgctgaat ccacgaacct
     7321 ctggcaggta catggcgtgg caatcgaatg taagcggctg aatttcctga tagtaaacaa
     7381 tgccgccaaa ctgaccgtac acgttaacct caaagtaacg gcattcaggc ttgtagtcgt
     7441 atccatcacc gttgttgctt ccggcaataa tgtcagggtg atttccgact gcttctatca
     7501 ggtcgatgtt tcgcgttggt ttgaactgaa tcattactgc tccgcgatta tcttgatggt
     7561 tgtggcagta aacgccgcac catttgactg aatggttaac gtgctgccat ttgtggcaag
     7621 aaagccgtct ttatccacgc tgaagaacgt agctaacagg atgttatcgg tcgttgtcgc
     7681 cgagttgcga ctgcttacca gtgtgtcagg aacagagccg gaaaatgtta gttgcattga
     7741 cctgttggcg gttccgctgg gccacgtccc gacgatcgac agcttgaaga acaaggtttt
     7801 gttctcgttg aacacaacca tcttgttgtt aacggtgtcg aagaatggtg ccaacgagcc
     7861 tgatgacggc gtgagcgttt tcagcaggct aacaaggttg gtcggcgctg tcgggatggt
     7921 tacagatacg ccagagtaaa caacctctga cttcttgcga gtagtggcat actccagagc
     7981 atcaatgcgc gattcatggt ctgaaacctg cgattccagc gactgaactc tggtatcaag
     8041 cgacgcaata tcgctttcat tctgagcgat tcgcgtttca tgttcctgaa gagttgattc
     8101 tgcctggctg attcgctcct catgattaac aagcgttgct tccgcagcag aaattcgctg
     8161 ctcatggtca gcgagaatca catcctgctc atcgttcctg acttgtgcgt cataagcgcc
     8221 ctgtccggcc tcgttggcct tgttagccac attaccaaca tcagtaccct gtgcgataac
     8281 gtaaagcaga tacgactgcg agaagatatt gcgtggaagg actgatgtgt cgagccgtgt
     8341 agcctgaatg attaccggca cattgagatt cgaatccgcc attactcaat ccttatctga
     8401 gcgccagaca gagtgacagg cgacttagtg ataacgcgca gtttgaagcc aacatttttc
     8461 ctgatgcgcc ctactcgctt ccacaaaacg cgtttgtcgt aaacgaacgg ttcattctgc
     8521 tcaatcatct gctcacgccc gtaatttatg ccgtcagtgg ttgcagagag gaacaggcgg
     8581 tcagcgtact gagcaacacc cgtcgatgat tccacctcca gatcaaagca tctggcgtta
     8641 tccgctttga acaacggagt aaacagcagg tgttcttgct gtagcccata ctggctgctg
     8701 atatcgaact gcaatttccc ggtaaccgat tccagcttat cgccgcacgt tatctgattt
     8761 ccttcgtaaa tgaagtcgat agcgcggtac acatcgtcat acaggcctgt tttcaacaca
     8821 caccattgcg gaccattggc gcttgaagat gcgtcgtaca cgaggacgtg gcgcggaagg
     8881 tggataatca gcagttcatg cgcatcaaat cgcagcgatt ccatcacacc atcagccagt
     8941 tcatcagcag tgtaggagcg aagaattttc tcaatgctcg cgctggcgat tggtgatacc
     9001 tgaccggagc cgatgatata cacagacggc gcacctgttg ccggattgct gatgaacgca
     9061 tacgaatcgg cgaacggcgt tttgcagtaa gtcccggcaa tgcctttctg caccatcagc
     9121 gatggctgtg cgacatacaa agcggcacca acggtggttg cccccgtcag ggagaaatat
     9181 tcaatcgtcg atgaaccaaa gcagacgatg aagtctcgcc atgttccgat gccgatgatg
     9241 ccgtcaggct gagactcggc tcgatattgt gcgctgtagc ggtcaggatg cgattcgtct
     9301 tcagggtcag tgataaacca tgaatcagtg ccgtcttttg accacgcata acgcccacgt
     9361 aaacgcgtaa tgtcgcgaac agaacctaac tcatactgca taaacccgct gtctgcaggc
     9421 cagtttgaga cggttttaac cgtgccatca tagcggtatt cgaccagttg cccgttaacg
     9481 cctaccgcct gtgatgttcg accatgcgcc attgatacgc gaccacttcc ggcaacatca
     9541 ccgacctcac tttcgccctt atacagtttg ccaccacaca cgcgataaac agcattctgt
     9601 gccatgttgt actcgacgcc tcgcgatacg ccgttcacat cagaacgttt ggcaatgccc
     9661 gggaatgagc gaagatatcc gctgctgttc aggatttctt tgggtgtagc caacatattc
     9721 actggcagat agtcgatata gtcggcgttt ctaaagtctt tgccgacacc tttcataagc
     9781 ggaagttgct gaataggcat ttattcacct atgcgtttgg gatatcgcca tcaatcagag
     9841 ggagatcgcc tggataatat cggtcagatg tgaacacgtc atatttatta ccctgcccta
     9901 caggaaaatc tccacgtcgt cgcattgaag gaacaaccag agtgtcggtc atcaaggcat
     9961 catatgagcg ttgggcgtta ctgagaactt gcggagttgg ttcaaggctg taatcagata
    10021 gcattctcag caataactga tagcctactg cgtgtttgta ttttcttgga agacctgact
    10081 catcatctgg taatggctgc tcatctccag ttgcgaaagc gtaaccaatg tcgccggggt
    10141 taatcatcca ctcggacatc atatcttcca gatcatttac accatcttca attgattgcg
    10201 gctcaacatc agtaagcgat gcattagaag caatagcaaa cttacgaagc gcaaaaagga
    10261 cgatctcacc ctttgtcagt actgttgcca ttgtccgccg ccttacgacc tcgcttactg
    10321 gtcggtttca attcatcaac tgaggcaaca aagcccaacc tttcgaaaaa ctggaagtct
    10381 ttttctgcga taacggcctg tacatgcccg gattcgttat ctgcggcaag gaatacactc
    10441 atccgatcca tattgtttcc ttaaaacata aaaggggcgt aagccccttg ttattacgga
    10501 ttaccgaaga actgaccgcc catgtgtggg ttaaagcaca catatgcagg cagtaagtcg
    10561 aagcgcattt tttgcacgtt ggcatcgcca tctgcgtatt tatgtacgcg gatggagaaa
    10621 ccttcatatg ttgcaacagc agaatcaata ctgtgcagtt tcggcagtgg gatagatcca
    10681 agtccacaga agaacttgtt atagaacagg tttggcttca ttgtctggct agcagtgcct
    10741 actacagata cggcatcgcc tgcctctacc tgacgactta cagagttgta ctgcgggttt
    10801 gtagtgtcat aaatcggaac accagaaagc gtaaccgtca catcgccact gctgtctgaa
    10861 ttagcatcag cagtaaccgt tgcagtgaag ctaattggtg tggctccgtt atacaacgcc
    10921 tgtttggtct gctgttgcag ccagtaggta ttggtgaatt tgacctgatc accagctttc
    10981 agaaaacctg taacgctggc tgtcgctccg gtcaatgtta cagtgaactg gtatgagtct
    11041 ttaactgcgt tataggtaac agttggctgt gttttgactg tcagtgttcc gccaaatgcc
    11101 ccctgcgtac gagaggcaag cccattagac atcagtgcgc gaatgccgcc aaaattggtt
    11161 gggatctgtg cgttctccca tgcagtacga accaattgat ctgaagcatg caaaccagtc
    11221 tgcgcatcag caagtcgctg tgcagaccat ggatccatta cagcatagtt ttcaccttca
    11281 ttaacgccga ggtctttcag gaaagatgcc gtctgcgcaa catcagacca tttggtgatt
    11341 ggagtattgg ggctaccaag tgacaacgca ccgttattca tcatgaagtg agcaagctct
    11401 gtttcaaggt cggtaacgat tcgctggcga accggcgcga gaatttcttc cagctggtta
    11461 agcttgatcg cttcctccag ttgctgatat tcaacagcaa cagtgatgta gttacctaca
    11521 cgccccgtag ctttacctga gatcaggttg tttttatttt gccctgaaat atcaccagtg
    11581 ggagtacgga gggatgagaa ttgatgcgga cgtttaaagc taacgctatc gccagtgctg
    11641 gagttgattt cacctgccag caactgacgg tctacggttt tcgccagaac taaatctgac
    11701 ataaaacccg gaaggaattt tttcagaacg atttgactga cgttactgtc gagattgtta
    11761 ggcatttatc ttttccttat tcgatttttg cgccggggca taatttgttg aattcgtctt
    11821 gtttcgcatc agcaccgcca ccacgtactt ccggctctgg cttgatggct ttctttggtt
    11881 ttggagcaag gcttacctgt ttgctaatct gccccaagag gaatgctgcg cgaattggat
    11941 ctgtctcagc ggctacacgc tggcgtaatt gctggctctt acctaagcca taggcgagta
    12001 gttcagagcc ttcgtctgca cagtgaatga tgatttcctg ctgaattggt ggtagctcac
    12061 taagaacaat ggcttccatt tcctgataat ctttcacagg aagtttggct gcccgttgtt
    12121 tatgcgcttc taccctttgc tggaaacgct gctggtattc ctgttgctga cgtagttttt
    12181 gttgctgctg ctgttcgaca cggccttttt tctcatgcca atcagtcaat gcctgttcaa
    12241 acgcctgttc gtcataatca cacgactcaa gagtcggttt tggtggaata gcgtctggtt
    12301 gtggttgctg atgttccgca tgcttggcta atgcttcctc aagctggcgt cgcaactcac
    12361 ggttttcttt ctgtgtttct ttgaagcctt tgcgaagatc tttcacccat tgcggtgcag
    12421 gttgcccgtc aatgtgatca ttatcgtcag cgttaagctg aatttcttca tcaccaatac
    12481 gcaaggcgta atcttctggt gtctcttcgg ttttttcagg atcagttgcc atctcttttc
    12541 cgttgtcatc ctggctttca ttctcaggct gtgactctgt ttggatgatg gtttcttctg
    12601 cattttcctg tgtttcagac aggtcaataa cctgaccgtc gatgatcagt tcgttttcca
    12661 ttgattactc ctggttaact cggcattaag tctgccggtg actgtggtgg tgactggaat
    12721 tgctgttgtt gtgactcggc gacatctttc agaaggcgta ttgcctccat cactgctttg
    12781 tcatcgatgt ttctggcttg agccagttta tagacagtgt ttgcctgact ctccatcgca
    12841 tcctgctggg cagtaaatgc tttgatttga gtttgagcag tttcgttagt tgctttttgc
    12901 gcttctgcct gcgctgctac catttgcgcc tgagcgagaa ccatttcagg atttggctgg
    12961 ctttgtgctg ccatttgcgc ctgttgaaca atctgctgct ctttctcatt gcgtggtttt
    13021 gcaataccag atatcagcag ttggtttcgg ttgtactctt tgaagtcatc aaggccttcg
    13081 ccatcgatat tgtccagaat aataccctga attgccgggc gcattgggtc tgttggaagc
    13141 atagagctaa ggacatttgt cagtacagaa accgttgcat cacgtcgtgc tgtgtagctt
    13201 ggtccaacat caaccgtcac atcgtatcga ccgacagaaa ggtcatttaa cgcaacaaca
    13261 gcccctgttt gcctgtcaac aacctgtgcg ctcaggacag cgatatcatc acttccatct
    13321 tcgttaacga tgcgcacttc acgctctgaa ccgtacactt cacgcgccat tgacagccat
    13381 acttcaccag cgcgtttaag acttttcgcc atattgtcca gatagataaa cgaagccata
    13441 tctgctctgt tcatcaagtt gttaaccgtt tcctgagcaa tattacttgg catctgctgc
    13501 atggcctgac tgccgcctgt aacctcctga atatcagcac tggtttgctg tagtaatgca
    13561 gccaatgcct gattcataac cgcaggctgt gtatatcctg ccggggtagc tccagcgata
    13621 atgttgccag atttatctct cacttcgcgc aacggcaaga acgctgggcg tttcttgttg
    13681 cgagcctccc agtgcttctc aagtccacga atttgctcca tgccaactat agggatctga
    13741 ccggggtctt gcgctgcagt atcagccagc attgatacct gaaggttata caaacgctgt
    13801 ggatccattg cttttgcaat atgtccttcg acacgctcaa tgtcatcaat gaaccagcgt
    13861 tttccataaa ccgggatgag ggggatatgc tcaccaggaa tacgtcgagg tttctcaagg
    13921 aaaccatcac catccactac ggatacatac acacgacggc gcttcactga gcgccttgcc
    13981 acttcctgaa atccagctat tgccagttca tcttcaatat cttcaacctg atcactgtcg
    14041 tatgttgcaa tctctccagt gattggatgt cgataactga tgacgacaac agactcttta
    14101 cgaacttcgt aatacttcgc tatgtaaata acatcttcat caaaccagtc atattcccaa
    14161 ctggtcatag acgttacatc cagagaagca ggaggtttct ttctgtattc agcctcatat
    14221 ttttcaggtg acaacgaata catgcagaac gcccacaacg cgtcagattt gtcgtacttc
    14281 ttagcgtcag ggtcaaacca cacagagcgc gacgggtcgt atattggttc aatagcaata
    14341 cgctgacgat cgtccatggg gtcgtattca ttgaccagca tcgacgtcaa acggaagcaa
    14401 ccgaaaccac cagtagcagc gtcgtcaaat gcattatcgc aagcctcacc gccatcggtt
    14461 tcttcgtagt cagcacggaa cagaccattt aatttattgg ctaactcttc gcttgcctct
    14521 ctgtcaccag gacgaaactt aacagtgatt ctgttattgc ggtattctgc aatgatgcgg
    14581 ttaagttcag ttgctacctt attgatttca aacttaggat acttctcgaa ctgctcatca
    14641 agcttagttc cagccgccgt tgctccttcc cattgacctc cggggacacg agcaaacctc
    14701 gtagcttcaa tgcacttttc gcgcacttcc tgctgtggag aataggcgcg atcaaacctg
    14761 agcatgatcc gctcatgttt tttctctaat gtctctgcca tgtttaccaa ccggaggatg
    14821 agggaacgta tatttctgtt tcttcgcgga ccaatgccgg gcaatgcata cacatcatca
    14881 gcgcatcagc caggttagga gatgggatac cgagcttctg cttcatttcg accttagtca
    14941 taagctccag cttcccgtta ttattgaatt tgcgctgaat ctgcgtcagt tctgcaaaca
    15001 gcttctccag catcttctcg cctatcgctt ctttgtcgaa gctcagcatg tcgtcggggt
    15061 ctgcatactc accgtggaca accgcccgat atgtcagata cagcctgtca gccagcgcgt
    15121 aatagaattg cgctcgctta ttgcggaata catcgccaat agtgcgaacg ttgtcgccct
    15181 gtacgacttc atcagcccat gctccggcct gataaggccc atcttcatcg aatggcgatt
    15241 cgctgccctt gaacatcgtg gcggtaattt tcttgccgga gaatgcttcc gttgtctgtc
    15301 tgcgtaggcc cgcaccgacg ccatcaccgt cccacaggta atggtcagcg ccgtcttcaa
    15361 tcgccagcga agtagcccag tcagcaccct cgttgatgtc catcagcaga ccttcggcaa
    15421 tgcgcttaac taccgaaccg tgacgcgatg cataaccttt agcatctggc cctgtatctg
    15481 aggggtcatg cgcagaaaca accgcacctt tcgctttcca cccgagtttc gtgtgcgcat
    15541 cggttgcggc ttcaagccat tcacgtttga tgattgccat atcacttgcg cttaccggct
    15601 caccaagcca gatgtgacga tacagtgtcg gatttctgcg tttacactct tccatctcca
    15661 gacggaggac ttcaggaaaa tgcgggttgt cggtgtagtt caccgtcagc aggcaaatat
    15721 catcgggagg atttacgacg aatcgctgat aggtatcgtc gaggatgttt ttcgggttga
    15781 aacttaccca tatttcagag aacggcttac ggatggttgg aatcaggata tcccacgatt
    15841 ccttcgttac cgcttccgct tcttccaccc agcagatatc aatgccttcg agcgatttaa
    15901 tcttcgtcgg gttgtttttg atgccgtaga acatgaattc agcattcgtt ccgagatgac
    15961 gaatcattga acgctgaatt tcaaactcag ccgaataccc ttcacgctcg atggtgtctt
    16021 caagcaacct gattaccgaa tcgctgatac tgttttgcag ctcacgagcg caaagaatac
    16081 gcacaggctg ccgacgcgcc gcttcaacaa gaagcctcgc aattgcccat gacttaccgc
    16141 tacctcgacc gcctttggcg actttgtagc gatgcgcctc aatgaacggt tcaaagatag
    16201 gattaatcga ggtcattttc cgaatagagt gctcatcggt gatgtttcaa tctggattgc
    16261 gccgccgtct ttgccgacaa gctcgttagt taccttgtcg ccatacttac ggggattcat
    16321 tcgggccagc gcccatttgc gggtatcaac gcgaagtctt gcctttgcca cctcggcggc
    16381 atcagggatt acgtcgtcag caatttcgaa tatctcttcg aaaatagagt cggcccgagt
    16441 ctctgttgcc ttcgcgtact ggtcacgaaa ctcctgatgt tcagccagcc agcgaaaaac
    16501 tgatgttttg ctcggcattc ctgggcgttc gcaaactttg cgcagactct caccggagga
    16561 aagcaatgcg caaatgtcat tagccacctc cggcatataa tcagaggggc gaccaccttt
    16621 ctttttctca gtcgccatat tgattatttc ccttctgctt gcttatccca ttcatcgcgg
    16681 aatttggatg ggttgtcgaa accttgagtt gccatgttta cgctccggta gtgaacaggt
    16741 ctaacgcttc cttcgattta cgcaccgctt cgattgtgcg ggtcgtgata tctgaattag
    16801 cgccacctga ctggaagtga attttgaata gctcaagctt cagctcgtca gtgccaatga
    16861 actgaaatgc ttcctccgcg gctgcgttct ggttcatgac cagtttgtaa atctctaact
    16921 ggaatttctg ttcttcagtc atgggaataa tctctgccat tgttggctcc gtttatccgt
    16981 taaaagggat atcagttaag ttatcccgtg tagggtataa gccattatca aagccactca
    17041 gtaaggaatg gcttttgtaa taactactgt tcgcttagct tctgcttcag caagtaacct
    17101 tcgagcatcc agattttgtt tacagcattc tgccgggcaa tcttccgacc aatttctgca
    17161 tcaaaatttt cctgacttgc acaggcactc tctccggtga cggtgaagcc gttgcgcagc
    17221 accaggacgc agaacgtcag cagagaaagt gattcgtgcg actggtagtt tacctctccg
    17281 ccagtatgtt tcgcttttat ggctttgcca aaggcaccat cctctgctgt gaaatatgcc
    17341 tcctgagcaa taattccttc gatatggtct ggcgtaactc gcggtgccgt tttgcctttc
    17401 tcaacgattt ctttttcgat ttgctggtcg ttcataatta tgaccctgta gagtggttgc
    17461 ttgattagga tgtctttcca tcagttcgcc accacaaaga atcttttttg ccataaggct
    17521 ggaggttcat ctttcagtgg ctgccagtgt tatttcccca cttactggct tgggttgttt
    17581 cgcggtactg ccgtaactgg tggtgcacag atttagttaa atctgttctc gcctgaacta
    17641 tcttttacat acccggattg tggggatgta aatcacggtt tcattatcaa gcccacccgt
    17701 agatgggctt tggaatggtc actttggcag tccggggatc gatatttgcg cctgctgctc
    17761 aagccttttg attcttgcta tgagttgcgg ttttttgatc ctgccccagc ggttcagcaa
    17821 gcgtcctgac atactggcaa catccttttc cttcatgaac tccagcatta actcgttgtg
    17881 ctctctttgg tatgagtgag ccatctccat cagcctgtca cgcatccaat taaatgcttt
    17941 gataaacgcc tctttgatgg cggcagcttt tttgccggta aacgacatga tgatgtacat
    18001 cgcaccgtct ttggaaattt catattcaac atactgatta cccttgtgtt cataggtaac
    18061 ccgcgaaaag ttgctggtta gaaattcatc cgaacagtct agcttttcga ttttctgaat
    18121 gatgtggtga tgctgcttgt cgaagtaagc tgctaccttg cgggaggttg tgatcacgcg
    18181 atcaccagaa acaaccacca tgtcccggaa atcgagatta gccaattgat gattcatagc
    18241 gtctttacct tttagaaagt gagcctgtct cacagaaaag ccgccccgag atggtcgcca
    18301 ccatatacgg cagttctcag gctcaacttt ctgaaaggct cgggtgatgt aatatgcgcg
    18361 tgagatgcgc tgtgaaattc agatgtaaaa aaagccccgc atcgcgaggc tcattaaatg
    18421 gactttgtga tttgcaaaaa aattatttca ggcactgagt cctgatgtac tcctgcaggt
    18481 agttaacctg cgcggttatc ctgtcgattc cacttcggag acggtaataa ttgagttcag
    18541 catctgctgt aagtcttggg ctttctccat cgcccatgct gctggctccg gtcgttgact
    18601 ttgcacaggt ggcggcgact tgcaggcgct tacgaccagc agaaacatca gcacgaaggc
    18661 tttcgatagt agcgttagca tcagcaagct cctttgtgta tctggcgtcg agttctgcta
    18721 catcacgttg acgcttctgc atatcagcga tgatggatgt ggctttatcg cgctgctctt
    18781 tgtaggtcat ggcgttatca cggtaatgat taacagccca tgacaggcag acgatgatgc
    18841 agataaccag agcggagata atcgcggtga ctctgctcat acctcaatct ctctgaccgt
    18901 tccgcccgct tctttgaatt ttgcaatcag gctgtcagcc ttatgctcga actgaccata
    18961 accagcgccc ggcagtgaag cccagatatt gctgcaacgg tcgattgcct gacgaatatc
    19021 accgcgatca atcatcggca aagcgccacg ctctttaatc tgctgcagcg caacagcgtc
    19081 ctggcttttg ggagagaagt ctttcaggcc aagctgctta cgataggcat cccaccaacg
    19141 ggaaagaagc tggtaacgtc cggcggctgt tgatttgagt tttgggttta gcgtgacaag
    19201 tttgcgagga tgatcggagt aatcagtgaa tagctctccg ccaacaatga cgtcataacc
    19261 atgatttctg gttttctgcc gtccattatc tgttccctct gaccacgcca gcatatcgag
    19321 gaacgccttg cgttgattat tgatttccac catcttctac tccggctttt ttagcagcga
    19381 agcgtttgat aagcgaacca atcgagtcag taccgatgta gccgatgaac acgctcgtta
    19441 tataagcgag attgctactt agtccggcga agtcgagaag gtcacgaatg aaccaggcga
    19501 taatggcaca catcgttgcg tcgattactg tttttgtaaa cgcaccgcca ttatatctgc
    19561 cgcgaaggta cgccattgca aacgcaagga ttgccccgat gccttgttcc tttgccgcga
    19621 gaatagcggc taacaggtca tgtttttctg gcatcttcat gtcttacccc cagaagggga
    19681 tctgttcaaa ttaggaatta tggatatggt cgcttgaaca aatccgggtt acggttgatt
    19741 tgtaacgggt ttgttcgtga ccgcattcat gagcaaatca ggcgtggatt gcgccaacaa
    19801 tacatgccgc tcatatcacg aagcccagcc attgatgctg ggttttcttt tttaaagcgt
    19861 actagacaac cgtatccaca gagtgtcagc aatgagttgg ttaggtctgg ttcttggtgg
    19921 aagtacgctt taaaaaatgg gctgatggtg tagcccaaaa tactgagtga atggtaagga
    19981 tgaacaacgg ttttgctctg ggtggatttg gctgtggtgg ccggcgctga tctccggctt
    20041 gtatacaggc accttgttct tccgaagctc tcctgcgcgc atcagcctgc gcattcacca
    20101 caccggaaag agcactcagt tgtaccggcc agttgtgcca ctaagaaatg ctttcgcaga
    20161 ccgttaagct ctttgccagt tctttaatgc tcttacctgt tgtgtgccca ttattaatca
    20221 caccgggcca gtgcgccgaa tttgtttaca aggagtcgga agaccttgct gacttacagg
    20281 ctattacgcc gccatcagaa caacatcatc gtttgcattt atctttgtgg tcagtttcta
    20341 aaaaaccgca aagtcgccaa ctctgacgaa aactatcgtt gtgctgccac aacgataaga
    20401 gcactcggtg catttaagcc aagccccata agggagaatg ctcttacctg ttacacagat
    20461 ataaaaaatc ccgaaaccgt tatgcaggct ctaactatta cctgcgaact gtttcgggat
    20521 tgcattttgc agacctctca gcctgcgatg gttggagttc cagacgatac gtcgaagtga
    20581 ccaactgggc ggaatcggta gtaagcgccg cctcttttta tctcaatacc acaacgagcg
    20641 aattaaccca tcgttgggtc aaatttaccc aactttattc aataagtcaa tatcatgccg
    20701 ttaatatgtt gccatccgtg gcaatcatgc tgctaacgtg tgaccgcgtt caaaatgttg
    20761 tctgcgattg actcttcctt gtggcattgc accaccagag cgtcatacag cggcttaaca
    20821 gtgcgtgacc aggtgggttg agtaaggttt gggattagca tcgttacagc gcgatatgcg
    20881 gcacttgctg gcatccttga atagccgacg cctttgcatc ttccgcactc tttctcaaca
    20941 actctccccc actgctctgt ttttgctata tcaaccgcac ggcctgtacc gtgacaatct
    21001 ctgcatcttg cgcccggcgt cgcggcacta cggcaataat ccgcataagc gaatgttgcg
    21061 agcacttgca gtacctttgc cttagtattt ccttcaagct ttgccacgcc acggtatttc
    21121 cccgatacct tgtgtgcaaa ttgcatcaga tagttgatag ccttttgttt gtcgttctgg
    21181 ctgagttcat gcttaccgca gaatgcagcc attccgaatc cggcttgtga ttgcgccatc
    21241 cccatagcag ccatcacatc agtaccggaa agagagtcag aagccgtagc ccgtggtgag
    21301 tcgctcatca tcgggctttt tggcgaatga aatttagcta cgctttcgag tctcatgcgc
    21361 cttctccctg tacatgaatc aatgtgaggt ttccgcagaa cactgcgccg gtatcgatat
    21421 acatctggtt ggcaaacttg agtggtttca ctgctggcgt atgaccaaag atgaacatgt
    21481 ccgctccttt gatttctttc acgatcccgt cttgtgagtt gctgattcgt tcgcggttcc
    21541 aaattaactg ctgatgatca actggctttc caaactcgta ttcgtcacaa ggataatcgg
    21601 cgtggcagat gacatatttt ttatctttgc tcaccagttc gatgattaac ggaagttcac
    21661 ctgctttatg ggcaagagct ttagccagaa tttctttgtc gtaatcgaga ttaaagaacc
    21721 agccaccgcc attaagcagc cagtgattga cgtttccacg ctctgataag ccatcaatca
    21781 tcatttgctc atggtttcca cgtacagctc tgaaccaggg gaatgtgatt aattccagac
    21841 attcgacgtt ctctgtaccg cgatcgacca aatcgccaac cgagataagc aggtcttttt
    21901 tggtgtcgaa tcctatcgtc tccagttttt tcatcaggtt cgtgtagcat ccgtgcagat
    21961 cgccaactac ccaaatattt cggtatttgc tgccatcaat tctttcgtaa tagcgcatct
    22021 ctttcactcc atccgcgatg aaccataaga acgtcgttga cgatggcgtg cattttcccg
    22081 tctttatcat caacgtattt tcttaccgtg ccgcgactac atttcagtct gcgtgctact
    22141 tctgtctggt ttccgtatgc ttcaacgagc atgtctggaa tggtttttac tgagaacgtc
    22201 atgcggcctc acttctgcta tttcgcaggt ctttgagttt ctgttggtac tctgccttga
    22261 tcgccttgca ctcttcgata gtccagcgat gcggctatgg tttgattcga tttcgtctac
    22321 tgcttcctgc cctatgcggt taatcagttc gacgcgatac ggaacgagat ttccgctttt
    22381 gtgctggttg cacaccacgc attgcttgtg aatattgcgt tcatcaaatc ggagttgagg
    22441 tgccgcagca gttgtccggt aatgtccggc atcccactga gcagacgtga gcgttccgca
    22501 cgagatacat ggtaagtcgc ggtctctttc tctgatgaag gcgtttacag cttgttgggc
    22561 ttgtttaatc cagtaactgc ggggctttaa ggcgagtttt cgaatcttaa gtttatcttt
    22621 ctgtttctgc tcctctcgtc gtcgtttctt ctctgctgct ttttccgctt tttcgcgttc
    22681 tttacttcgt cgttcgagtg ctaattgagt tccgtgttcc gggcagcacc accactgatt
    22741 tgagaatgcc gggtgaaacc attccttgca tattttgcat ttccttctcg ctggtttagc
    22801 catcgtattc ttcctcgtac attgagctat tcggatcgct catcagttct gcacagcagt
    22861 gctcacacac gtgaacttcc agcacatgca gcttctgacc gcagttagcg cacgttaaag
    22921 ctcgctcgac gctttctttc tggtattgaa gggatt
//
LOCUS       DAZKHF020000055        22798 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2201S31241
            SAMN43267800-rid25106413.denovo.055, whole genome shotgun sequence.
ACCESSION   DAZKHF020000055 DAZKHF020000000
VERSION     DAZKHF020000055.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267800
            Sequence Read Archive: SRR30307263
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 22798)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22798)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 22798)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350581.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350581.2
            Genome Coverage       :: 151x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:21:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,652
            CDSs (total)                      :: 5,557
            Genes (coding)                    :: 5,320
            CDSs (with protein)               :: 5,320
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 237
            CDSs (without protein)            :: 237
            Pseudo Genes (ambiguous residues) :: 0 of 237
            Pseudo Genes (frameshifted)       :: 102 of 237
            Pseudo Genes (incomplete)         :: 130 of 237
            Pseudo Genes (internal stop)      :: 52 of 237
            Pseudo Genes (multiple problems)  :: 41 of 237
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22798
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267800-rid25106413.denovo.055"
                     /serotype="O 26:H11"
                     /isolate="2201S31241"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-01"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            <1..388
                     /gene="yfdR"
                     /locus_tag="ACHOSC_RS08575"
                     /old_locus_tag="pgaptmp_001715"
     CDS             <1..388
                     /gene="yfdR"
                     /locus_tag="ACHOSC_RS08575"
                     /old_locus_tag="pgaptmp_001715"
                     /EC_number="3.1.3.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416862.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="5'-deoxynucleotidase"
                     /protein_id="HGU4547676.1"
                     /translation="AVLCSQLVPQEFAFEALMHDATEAYCQDIPAPLKRLLPDYKRME
                     EKIDAVIREKYGLPPVMSTPVKYADLIMLATERRDLGLDDGSFWPVLEGIPATEMFKV
                     IPLSPGHAYGMFMERFNELSGLRKCA"
     gene            379..525
                     /locus_tag="ACHOSC_RS08580"
                     /old_locus_tag="pgaptmp_001716"
                     /pseudo
     CDS             379..525
                     /locus_tag="ACHOSC_RS08580"
                     /old_locus_tag="pgaptmp_001716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416863.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            502..966
                     /locus_tag="ACHOSC_RS08585"
                     /old_locus_tag="pgaptmp_001717"
                     /pseudo
     CDS             502..966
                     /locus_tag="ACHOSC_RS08585"
                     /old_locus_tag="pgaptmp_001717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311034.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            968..1159
                     /locus_tag="ACHOSC_RS08590"
                     /old_locus_tag="pgaptmp_001718"
     CDS             968..1159
                     /locus_tag="ACHOSC_RS08590"
                     /old_locus_tag="pgaptmp_001718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4547677.1"
                     /translation="MNGQISIVRPGACDDSEIRMIIRLARGKTITVLITPENLALALT
                     GKSDLPVELKLRNVEIKVK"
     gene            1162..1752
                     /locus_tag="ACHOSC_RS08595"
                     /old_locus_tag="pgaptmp_001719"
     CDS             1162..1752
                     /locus_tag="ACHOSC_RS08595"
                     /old_locus_tag="pgaptmp_001719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000206819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF551 domain-containing protein"
                     /protein_id="HGU4547678.1"
                     /translation="MTTFTNSKLTDEYVSNATLIRLILWADQHNSHYVVAALRELQER
                     RKADSAEPVSQTYKLPVNTPCQDAPAHIWLQTAGVWPEDGELSELTWCSHNQHHDDTL
                     YVRADLVNGNYPATPDGWISCSERMPEMGERQCYVLAADFKNNYPPNIPNTQVGVYGD
                     WFNDGKPTWDDGDGEDLYLKEVTHWMQLPEPPQEVK"
     gene            1840..2082
                     /locus_tag="ACHOSC_RS08600"
                     /old_locus_tag="pgaptmp_001720"
     CDS             1840..2082
                     /locus_tag="ACHOSC_RS08600"
                     /old_locus_tag="pgaptmp_001720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4222 domain-containing protein"
                     /protein_id="HGU4547679.1"
                     /translation="MFALIQRGQIYTDRAGYPVVITRSTQHSVFFRRMDGRSGRVRIG
                     EFNNLFEHIDQQEYRKILAGTEQEMRLKKLRAMQRR"
     gene            2086..2220
                     /locus_tag="ACHOSC_RS08605"
                     /old_locus_tag="pgaptmp_001721"
     CDS             2086..2220
                     /locus_tag="ACHOSC_RS08605"
                     /old_locus_tag="pgaptmp_001721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4547680.1"
                     /translation="MHTAFEFWVRKTFGNRYDLTRDVDGFYCREVAKRMFDVWCHCRG
                     "
     gene            2239..2493
                     /gene="xisR"
                     /locus_tag="ACHOSC_RS08610"
                     /old_locus_tag="pgaptmp_001722"
     CDS             2239..2493
                     /gene="xisR"
                     /locus_tag="ACHOSC_RS08610"
                     /old_locus_tag="pgaptmp_001722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excisionase family protein"
                     /protein_id="HGU4547681.1"
                     /translation="MQTIIYQITPSKWCTERVLIASTGLKPGTIERARRKSWMQGKEY
                     RHYAVEGDPGHYSECLYNIEEIMRWIENQKQPGAKNASSG"
     gene            2527..3814
                     /locus_tag="ACHOSC_RS08615"
                     /old_locus_tag="pgaptmp_001723"
                     /pseudo
     CDS             2527..3814
                     /locus_tag="ACHOSC_RS08615"
                     /old_locus_tag="pgaptmp_001723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311040.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
     gene            3835..4536
                     /gene="mlrA"
                     /locus_tag="ACHOSC_RS08620"
                     /old_locus_tag="pgaptmp_001724"
     CDS             3835..4536
                     /gene="mlrA"
                     /locus_tag="ACHOSC_RS08620"
                     /old_locus_tag="pgaptmp_001724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator MlrA"
                     /protein_id="HGU4547682.1"
                     /translation="MSLPLIPVTLRAWQRRYGLLKPQRTDGGHRLFNDADIDRIREIK
                     RWIDNGVQVSKVKMLLSNENVDVQNGWRDQQETLLTYLQSGNLHSLRTWIKERGQDYP
                     AQTLTTHLFIPLRRRLQCQQPTLQALLAILDGVLINYIAICLASARKKQGKDALVVGW
                     NIQDTTRLWLEGWIASQQGWRIDVLAHSLNQLRPELFEGRTLLVWCGENRTSAQQQQL
                     TSWQEQGHDIFPLGI"
     gene            4596..4703
                     /gene="yohO"
                     /locus_tag="ACHOSC_RS08625"
                     /old_locus_tag="pgaptmp_001725"
     CDS             4596..4703
                     /gene="yohO"
                     /locus_tag="ACHOSC_RS08625"
                     /old_locus_tag="pgaptmp_001725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YohO"
                     /protein_id="HGU4547683.1"
                     /translation="MRIAKIGVIALFLFMAFGGIGGVMLAGYTFILRAG"
     gene            complement(4684..5415)
                     /gene="yehW"
                     /locus_tag="ACHOSC_RS08630"
                     /old_locus_tag="pgaptmp_001726"
     CDS             complement(4684..5415)
                     /gene="yehW"
                     /locus_tag="ACHOSC_RS08630"
                     /old_locus_tag="pgaptmp_001726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine ABC transporter permease YehW"
                     /protein_id="HGU4547684.1"
                     /translation="MKMLRDPLFWLIALFVALIFWLPYSQPLFAALFPQLPRPVYQQE
                     SFAALALAHFWLVGISSLFAVIIGTGAGIAVTRPWGAEFRPLVETIAAVGQTFPPVAV
                     LAIAVPVIGFGLQPAIIALILYGVLPVLQATLAGLGAIDASVTEVAKGMGMSRGQRLR
                     KVELPLAAPVILAGVRTSVIINIGTATIASTVGASTLGTPIIIGLSGFNTAYVIQGAL
                     LVALAAIIADRLFERLVQALSQHAK"
     gene            complement(5420..6346)
                     /gene="yehX"
                     /locus_tag="ACHOSC_RS08635"
                     /old_locus_tag="pgaptmp_001727"
     CDS             complement(5420..6346)
                     /gene="yehX"
                     /locus_tag="ACHOSC_RS08635"
                     /old_locus_tag="pgaptmp_001727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine ABC transporter ATP binding
                     protein YehX"
                     /protein_id="HGU4547685.1"
                     /translation="MIEFSHVSKLFGAQKAVNDLNLNFQEGSFSVLIGTSGSGKSTTL
                     KMINRLVEHDSGEIRFAGEEIRSLPVLELRRRMGYAIQSIGLFPHWSVAQNIATVPQL
                     QKWSRARIDDRIDELMTLLGLESNLRERYPHQLSGGQQQRVGVARALAADPQVLLMDE
                     PFGALDPVTRGALQQEMTRIHRLLGRTIVLVTHDIDEALRLAEHLVLMDHGEVVQQGN
                     PLTMLTRPANDFVRQFFGRSELGVRLLSLRSVADYVRREERAEGEALAEEMTLRDALS
                     LFVARGCEVLPVVNTQGQPCGTLHFQDLLVEA"
     gene            complement(6339..7496)
                     /gene="yehY"
                     /locus_tag="ACHOSC_RS08640"
                     /old_locus_tag="pgaptmp_001728"
     CDS             complement(6339..7496)
                     /gene="yehY"
                     /locus_tag="ACHOSC_RS08640"
                     /old_locus_tag="pgaptmp_001728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine ABC transporter permease YehY"
                     /protein_id="HGU4547686.1"
                     /translation="MTYLRINPVLALLLLLTAIAAALPFISYAPNRLVSGEGRHLWQL
                     WPQTIWMLVGVGCAWLTACFIPGKKGSICALILAQFVFVLLVWGAGKAATQLAQNGSA
                     LARTSLGSGFWLAAALALLACSDAIRRISTHPLWRWLLHMQIAIIPLWLLYSGTLNDL
                     SLMKEYANRQDVFDDALAQHLTLLFGAVLPALVIGVPLGIWCYFSTARQGAIFSLLNV
                     IQTVPSVALFGLLIAPLAALVTAFPWLGKLGIAGTGMTPALIALVLYALLPLVRGVVV
                     GLNQIPRDVLESARAMGMSGAQRFLHVQLPLALPVFLRSLRVVMVQTVGMAVIAALIG
                     AGGFGALVFQGLLSSAIDLVLLGVIPVIVLAVLIDALFDLLIALLKVKRND"
     gene            complement(7503..8420)
                     /gene="osmF"
                     /locus_tag="ACHOSC_RS08645"
                     /old_locus_tag="pgaptmp_001729"
     CDS             complement(7503..8420)
                     /gene="osmF"
                     /locus_tag="ACHOSC_RS08645"
                     /old_locus_tag="pgaptmp_001729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine ABC transporter
                     substrate-binding protein OsmF"
                     /protein_id="HGU4547687.1"
                     /translation="MPLLKLWAGSLVMLAAVSLPLQAASPVKVGSKIDTEGALLGNII
                     LQVLESHGVPTVNKVQLGTTPVVRGAITSGELDIYPEYTGNGAFFFKDENDAAWKNAQ
                     QGYEKVKKLDSEHNKLIWLTPAPANNTWTIAVRQDVAEKNKLTSLADLSRYLQEGGTF
                     KLAASAEFIERADALPAFEKAYGFKLGQDQLLSLAGGDTAVTIKAAAQQTSGVNAAMA
                     YGTDGSVAALGLQTLSDPQGVQPIYAPAPVVRESVLKEYPQMAQWLQPVFASLDAKTL
                     QQLNASIAVEGLDAKKVAADYLKQKGWAK"
     gene            complement(8631..10928)
                     /gene="bglX"
                     /locus_tag="ACHOSC_RS08650"
                     /old_locus_tag="pgaptmp_001730"
     CDS             complement(8631..10928)
                     /gene="bglX"
                     /locus_tag="ACHOSC_RS08650"
                     /old_locus_tag="pgaptmp_001730"
                     /EC_number="3.2.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-glucosidase BglX"
                     /protein_id="HGU4547688.1"
                     /translation="MKWLCSVGIAVSLALQPALADDLFGNHPLTPEARDAFVTELLKK
                     MTVDEKIGQLRLISVGPDNPKEAIREMIKDGQVGAIFNTVTRQDIRAMQDQVMELSRL
                     KIPLFFAYDVLHGQRTVFPISLGLASSFNLDAVKTVGRVSAYEAADDGLNMTWAPMVD
                     VSRDPRWGRASEGFGEDTYLTSTMGKTMVEAMQGKSPADRYSVMTSVKHFAAYGAVEG
                     GKEYNTVDMSPQRLFNDYMPPYKAGLDAGSGAVMVALNSLNGTPATSDSWLLKDVLRD
                     QWGFKGITVSDHGAIKELIKHGTAADPEDAVRVALKSGINMSMSDEYYSKYLPGLIKS
                     GKVTMEELDDAARHVLNVKYDMGLFNDPYSHLGPKESDPVDTNAESRLHRKEAREVAR
                     ESLVLLKNRLETLPLKKSATIAVVGPLADSKRDVMGSWSAAGVADQSVTVLTGIKNSV
                     GENGKVLYAKGANVTSDKGIIDFLNQYEEAVKVDPRSPQEMIDEAVQTAKQSDVVVAV
                     VGEAQGMAHEASSRTDITIPQSQRDLIAALKATGKPLVLVLMNGRPLALVKEDQQADA
                     ILETWFAGTEGGNAIADVLFGDYNPSGKLPMSFPRSVGQIPVYYSHLNTGRPYNADKP
                     NKYTSRYFDEANGALYPFGYGLSYTTFTVSDVKLSAPTMKRDGKVTASVQVTNTGKRE
                     GATVVQMYLQDVTASMSRPVKQLKGFEKITLKPGETQTVSFPIDIEALKFWNQQMKYD
                     AEPGKFNVFIGTDSARVKKGEFELL"
     gene            11124..12839
                     /gene="dld"
                     /locus_tag="ACHOSC_RS08655"
                     /old_locus_tag="pgaptmp_001731"
     CDS             11124..12839
                     /gene="dld"
                     /locus_tag="ACHOSC_RS08655"
                     /old_locus_tag="pgaptmp_001731"
                     /EC_number="1.1.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016901 - oxidoreductase activity, acting
                     on the CH-OH group of donors, quinone or similar compound
                     as acceptor [Evidence IEA];
                     GO_process: GO:0006089 - lactate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0019516 - lactate oxidation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-lactate dehydrogenase"
                     /protein_id="HGU4547689.1"
                     /translation="MSSMTTTDNKAFLNELARLVGHSHLLTDPAKTARYRKGFRSGQG
                     DALAVVFPGSLLELWRVLKACVTADKIILMQAANTGLTEGSTPNGNDYDRDIVIISTL
                     RLDKLHVLGKGEQVLAYPGTTLYSLEKALKPLGREPHSVIGSSCIGASVIGGICNNSG
                     GSLVQRGPAYTEMSLFARINEDGKLTLVNHLGIDLGETPEQILSKLDDDRIKDDDVRH
                     DGRHAHDYDYVHRVRDIEADTPARYNADPDRLFESSGCAGKLAVFAVRLDTFEAEKNQ
                     QVFYIGTNQPEVLTEIRRHILANFENLPVAGEYMHRDIYDIAEKYGKDTFLMIDKLGT
                     DKMPFFFNLKGRTDAMLEKVKFFRPHFTDRAMQKFGHLFPSHLPPRMKNWRDKYEHHL
                     LLKMAGDGVGEAKSWLVDYFKQAEGDFFVCTPEEGSKAFLHRFAAAGAAIRYQAVHSD
                     EVEDILALDIALRRNDTEWYEHLPPEIDSQLVHKLYYGHFMCYVFHQDYIVKKGVDVH
                     ALKEQMLELLQQRGAQYPAEHNVGHLYKAPETLQKFYRENDPTNSMNPGIGKTSKRKN
                     WQEVE"
     gene            complement(12877..13809)
                     /gene="pbpG"
                     /locus_tag="ACHOSC_RS08660"
                     /old_locus_tag="pgaptmp_001732"
     CDS             complement(12877..13809)
                     /gene="pbpG"
                     /locus_tag="ACHOSC_RS08660"
                     /old_locus_tag="pgaptmp_001732"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311048.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009002 - serine-type D-Ala-D-Ala
                     carboxypeptidase activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine endopeptidase"
                     /protein_id="HGU4547690.1"
                     /translation="MPKFRVSLFSLALMLAVPLAPQAVAKTAAATTASQPEIASGSAM
                     IVDLNTNKVIYSNHPDLVRPIASISKLMTAMVVLDARLPLDEKLKVDISQTPEMKGVY
                     SRVRLNSEISRKDMLLLALMSSENRAAASLAHHYPGGYKAFIKAMNAKAKSLGMNNTR
                     FVEPTGLSVHNVSTARDLTKLLIASKQYPLIGQLSTTREDMATFSNPTYTLPFRNTNH
                     LVYRDNWNIQLTKTGFTNAAGHCLVMRTVINNKPVALVVMDAFGKYTHFADASRLRTW
                     IETGKVMPVPAAALSYKKQKAAQMAAAGQTAQND"
     gene            complement(13983..14570)
                     /gene="yohC"
                     /locus_tag="ACHOSC_RS08665"
                     /old_locus_tag="pgaptmp_001733"
     CDS             complement(13983..14570)
                     /gene="yohC"
                     /locus_tag="ACHOSC_RS08665"
                     /old_locus_tag="pgaptmp_001733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014170891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0031267 - small GTPase binding [Evidence
                     IEA];
                     GO_process: GO:0006888 - endoplasmic reticulum to Golgi
                     vesicle-mediated transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Yip1 family protein"
                     /protein_id="HGU4547691.1"
                     /translation="MSHVWGLFSHPDREMQVINRENETISHHYTHHVLLMAAIPVICA
                     FIGTTQIGWNFGDGTILKLSWFTGLALAVLFYGVMLAGVAVMGRVIWWMARNYPQRPS
                     LAHCMVFAGYVATPLFLSGLVALYPLVWLCALVGTVALFYTGYLLYLGIPSFLNINKE
                     EGLSFSSSTLAIGVLVLEVLLALTVILWGYGYRLF"
     gene            14740..15318
                     /gene="yohD"
                     /locus_tag="ACHOSC_RS08670"
                     /old_locus_tag="pgaptmp_001734"
     CDS             14740..15318
                     /gene="yohD"
                     /locus_tag="ACHOSC_RS08670"
                     /old_locus_tag="pgaptmp_001734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416640.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HGU4547692.1"
                     /translation="MDLNTLISQYGYAALVIGSLAEGETVTLLGGVAAHQGLLKFPLV
                     VLSVALGGMIGDQVLYLCGRRFGGKLLRRFSKHQDKIERAQKLIQRHPYLFVIGTRFM
                     YGFRVIGPTLIGASQLPPKIFLPLNILGAFAWALIFTTIGYAGGQVIAPWLHNLDQHL
                     KHWVWLILVVVLVVGVRWWLKRRGKKKPDNQA"
     gene            complement(15448..16209)
                     /gene="yohF"
                     /locus_tag="ACHOSC_RS08675"
                     /old_locus_tag="pgaptmp_001735"
     CDS             complement(15448..16209)
                     /gene="yohF"
                     /locus_tag="ACHOSC_RS08675"
                     /old_locus_tag="pgaptmp_001735"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HGU4547693.1"
                     /translation="MAQVAIITASDSGIGKECALLLAQQGFDIGITWHSDEEGAKDTA
                     REVVSHGVRAEIVQLDLGKLPEGAQALEKLIQRLGRIDVLVNNAGAMTKAPFLDMAFD
                     EWRKIFTVDVDGAFLCSQIAARQMVKQGQGGRIINITSVHEHTPLPDASAYTAAKHAL
                     GGLTKAMALELVRHKILVNAVAPGAIATPMNGMDDSDVKPDAEPSIPLRRFGATYEIA
                     SLVAWLCSEGANYTTGQSLIVDGGFMLANPQFNPE"
     gene            complement(16262..17698)
                     /gene="mdtQ"
                     /locus_tag="ACHOSC_RS08680"
                     /old_locus_tag="pgaptmp_001736"
     CDS             complement(16262..17698)
                     /gene="mdtQ"
                     /locus_tag="ACHOSC_RS08680"
                     /old_locus_tag="pgaptmp_001736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001583680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug resistance outer membrane protein
                     MdtQ"
                     /protein_id="HGU4547694.1"
                     /translation="MNRDSFYPAIACFPLLLMLAGCAPMHETRQALSQQTPAAQVDTA
                     LPTALKNGWPDSQWWLEYHDNQLTSLINNALQNAPDMQVAEQRIQLAEAQAKAIATQD
                     GPQIDFSADMERQKMSAEGLMGPFALNDPAAGTTGPWYTNGTFGLTAGWHLDIWGKNR
                     AEVTARLGTVKARAAEREQTRQLLAGSVARLYWEWQTQAALNTVLQQIEKEQNTIIAT
                     DRQLYQNGITSSVEGVETDINASKTRQQLNDVAGKMKIIEARLSALTNNQTKSLKLKP
                     VALPKVASQLPDELGYSLLARRADLQAAHWYVESSLSTIDAAKAAFYPDINLMAFLQQ
                     DALHLSDLFRHSAQQMGVTAGLTLPIFDSGRLNANLDIAKAESNLSIASYNKAVVEAV
                     NDVARAASQVQTLAEKNQHQAQIERDALRVVGLAQARFNAGIIAGSRVSEARIPALRE
                     RANGLLLQGQWLDASIQLTGALGGGYKR"
     gene            17922..18005
                     /gene="yohP"
                     /locus_tag="ACHOSC_RS08685"
                     /old_locus_tag="pgaptmp_001737"
     CDS             17922..18005
                     /gene="yohP"
                     /locus_tag="ACHOSC_RS08685"
                     /old_locus_tag="pgaptmp_001737"
                     /inference="COORDINATES: protein motif:HMM:NF011345.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="small membrane protein YohP"
                     /protein_id="HGU4547695.1"
                     /translation="MKIILWAVLIIFLIGLLVVTGVFKMIF"
     gene            complement(18393..19343)
                     /gene="dusC"
                     /locus_tag="ACHOSC_RS08690"
                     /old_locus_tag="pgaptmp_001738"
     CDS             complement(18393..19343)
                     /gene="dusC"
                     /locus_tag="ACHOSC_RS08690"
                     /old_locus_tag="pgaptmp_001738"
                     /EC_number="1.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0017150 - tRNA dihydrouridine synthase
                     activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0002943 - tRNA dihydrouridine synthesis
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA dihydrouridine(16) synthase DusC"
                     /protein_id="HGU4547696.1"
                     /translation="MRVLLAPMEGVLDSLVRELLTEVNDYDLCITEFVRVVDQLLPVK
                     VFHRICPELQNASRTPSGTLVRVQLLGQFPQWLAENAARAVELGSWGVDLNCGCPSKT
                     VNGSGGGATLLKDPELIYQGAKAMREAVPAHLPVSVKVRLGWDSGEKKFEIADAVQQA
                     GATELVVHGRTKEQGYRAEHIDWQAIGEIRQRLNIPVIANGEIWDWQSAQQCIAISGC
                     DAVMIGRGALNIPNLSRVVKYNEPRMPWPEVVALLQKYTRLEKQGDTGLYHVARIKQW
                     LSYLRKEYDEATELFQHVRVLNNSPDIARAIQAIDIDKLR"
     gene            19582..19980
                     /gene="yohJ"
                     /locus_tag="ACHOSC_RS08695"
                     /old_locus_tag="pgaptmp_001739"
     CDS             19582..19980
                     /gene="yohJ"
                     /locus_tag="ACHOSC_RS08695"
                     /old_locus_tag="pgaptmp_001739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CidA/LrgA family protein"
                     /protein_id="HGU4547697.1"
                     /translation="MSKTLNIIWQYLRAFVLIYACLYAGIFIASLLPVTIPGSIIGML
                     ILFVLLALQILPAKWVNPGCYVLIRYMALLFVPIGVGVMQYFDLLRAQFGPVVVSCAI
                     STLVVFLVVSWSSQLVHGERKVVGQKGSEE"
     gene            19977..20672
                     /gene="yohK"
                     /locus_tag="ACHOSC_RS08700"
                     /old_locus_tag="pgaptmp_001740"
     CDS             19977..20672
                     /gene="yohK"
                     /locus_tag="ACHOSC_RS08700"
                     /old_locus_tag="pgaptmp_001740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CidB/LrgB family autolysis modulator"
                     /protein_id="HGU4547698.1"
                     /translation="MMANIWWSLPLTLIVFFAARKLAARYKFPLLNPLLVAMVVIIPF
                     LMLTGISYDSYFKGSEVLNDLLQPAVVALAYPLYEQLHQIRARWKSIITICFIGSVVA
                     MVTGTSVALLMGASPEIAASILPKSVTTPIAMAVGGSIGGIPAISAVCVIFVGILGAV
                     FGHTLLNAMRIRTKAARGLAMGTASHALGTARCAELDYQEGAFSSLALVLCGIITSLI
                     APFLFPIILAVMG"
     gene            20802..21686
                     /gene="cdd"
                     /locus_tag="ACHOSC_RS08705"
                     /old_locus_tag="pgaptmp_001741"
     CDS             20802..21686
                     /gene="cdd"
                     /locus_tag="ACHOSC_RS08705"
                     /old_locus_tag="pgaptmp_001741"
                     /EC_number="3.5.4.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004126 - cytidine deaminase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0009972 - cytidine deamination [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytidine deaminase"
                     /protein_id="HGU4547699.1"
                     /translation="MHPRFQTAFAQLADNLQSALEPILADKYFPALLTGEQVSSLKSA
                     TGLDEDALAFALLPLAAACARTPLSNFNVGAIARGVSGTWYFGANMEFIGATMQQTVH
                     AEQSAISHAWLSGEKALAAITVNYTPCGHCRQFMNELNSGLDLRIHLPGREAHALRDY
                     LPDAFGPKDLEIKTLLMDEQDHGYALTGDALSQAAIAAANRSHMPYSKSPSGVALECK
                     DGRIFSGSYAENAAFNPTLPPLQGALILLNLKGYDYPDIQRAVLAEKADAPLIQWDAT
                     SATLKALGCHSIDRVLLA"
     gene            21836..22555
                     /gene="sanA"
                     /locus_tag="ACHOSC_RS08710"
                     /old_locus_tag="pgaptmp_001742"
     CDS             21836..22555
                     /gene="sanA"
                     /locus_tag="ACHOSC_RS08710"
                     /old_locus_tag="pgaptmp_001742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane permeability protein SanA"
                     /protein_id="HGU4547700.1"
                     /translation="MLKRVFLSLLVLIGLLLLTVLGLDRWMSWKTAPYIYDELQDLPY
                     RQVGVVLGTAKYYRTGVINQYYRYRIQGAINAYNSGKVNYLLLSGDNALQSYNEPMTM
                     RKDLIAAGVDPSDIVLDYAGFRTLDSIVRTRKVFDTNDFIIITQRFHCERALFIALHM
                     GIQAQCYAVPSPKDMLSVRIREFAARFGALADLYIFKREPRFLGPLVPIPAMHQVPED
                     AQGYPAVTPEQLLELQKKQGK"
     gene            22558..22797
                     /gene="yeiS"
                     /locus_tag="ACHOSC_RS08715"
                     /old_locus_tag="pgaptmp_001743"
     CDS             22558..22797
                     /gene="yeiS"
                     /locus_tag="ACHOSC_RS08715"
                     /old_locus_tag="pgaptmp_001743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2542 family protein"
                     /protein_id="HGU4547701.1"
                     /translation="MDVQQFFVVAVFFLIPIFCFREAWKGWRAGAIDKRVKNAPEPVY
                     VWRAKNPGLFFAYMVAYIGFGILSIGMIVYLIFYR"
BASE COUNT         5574 a         6010 c         5769 g         5445 t
ORIGIN      
        1 tgcggtgctt tgcagccagc tggtaccgca ggaatttgct tttgaagcgt taatgcatga
       61 tgcaacagaa gcatattgcc aggacatccc cgcaccactg aaacgccttc ttcctgacta
      121 taaacggatg gaagaaaaaa tagacgccgt aatccgtgag aaatacgggt tacctcctgt
      181 tatgagcacg ccagtgaaat atgccgatct cattatgctg gcaaccgaac gccgcgatct
      241 cgggcttgat gatggttctt tctggcctgt actggaaggt atcccggcga cagagatgtt
      301 caaagttatt ccactgtcac caggccatgc ctacgggatg tttatggaac gttttaacga
      361 gttatcgggg ttacgcaaat gcgcatgaat gttttcgaaa tggaagggtt tcttcgcggg
      421 aaatgtgtac cgcgagatct gaaagtgaac gaaacaaacg ctgagtacct agtgcgtaaa
      481 tttgctgaag ctgaggccaa gtgcgcggcg ctggctgaac gtatcgaaga gttacagaca
      541 aaacctacgc cagattcgtt tggcatcatc ggtgaaaata ttcgaacaca ggataatcga
      601 ataacgtcag accctatgtt ttgtgtgtat caaaagcgcg aaattgttgt tgatgctgat
      661 tatgactatg accggattgt ctgggttgat gaagatggca atgaagccaa taaactccaa
      721 agtcgtcgtc tcgaactact tcatgaaaac tttcgggaac caccagaaaa atggcggcgc
      781 gttgctgtga aagatattga tgaattcgtt acctgctgtt tcaccgaaca gggttgtaaa
      841 gactacctgg cagccaatgg tcacaatctt cgcttgccat ttatatatgt aaaaagcggt
      901 ttcaggaacg ctgaatatat cggcataaga acctggcttg ctggcattcg catcaaagga
      961 gagtgagatg aacggacaaa tatcaattgt tcgaccagga gcatgtgacg atagcgagat
     1021 acgcatgatt attcgtctgg cgagggggaa aacaataact gttctcatta ctccagaaaa
     1081 tctcgcatta gcattaacag gaaagtcaga cctgccagta gagctaaagc tgcgaaatgt
     1141 tgagattaag gtgaaatagc tatgaccact tttaccaata gcaaactaac agacgaatac
     1201 gtttcaaatg caacgttgat tcggctcatt ctgtgggctg accagcacaa tagccattat
     1261 gttgtagcgg ctctgcgcga gctacaggag cgccgcaagg ctgatagtgc agaacctgta
     1321 agccaaactt acaagttgcc agttaataca ccttgccaag atgcgccagc ccatatctgg
     1381 ctgcaaacgg ctggagtatg gccagaagat ggcgagttaa gcgaattaac gtggtgcagc
     1441 cacaatcaac accatgatga cacgctatat gttcgagctg accttgtgaa tggcaactat
     1501 ccggcaactc cggatggttg gataagctgt agtgagcgaa tgcctgaaat gggagagcga
     1561 caatgctatg tgttagcagc tgactttaaa aacaactacc caccaaacat ccccaacact
     1621 caggtcggcg tatatggcga ctggtttaat gatggcaagc ccacttggga tgacggtgat
     1681 ggcgaagacc tgtatctcaa agaggtaacc cactggatgc agctaccaga accgccgcag
     1741 gaggtgaaat gatgaattgg cctgaagcat tcaccgcagt tggagttgca atagcggtgg
     1801 catttattct gtattcgctt ttccgctggg gataaaggaa tgttcgctct gattcaacgt
     1861 ggtcagatat acacggacag agccggatac cctgtggtga ttactcgcag tactcagcac
     1921 tcagtgttct ttcgacgcat ggacgggcgc tccggacggg tacgcattgg tgagttcaac
     1981 aacctgttcg aacatattga ccaacaggag taccgcaaaa ttctggcggg cactgagcag
     2041 gaaatgcgcc tgaaaaaatt acgcgcaatg caacggaggt gatacatgca tacggctttt
     2101 gagttctggg ttcgcaagac attcggcaat cgctacgacc tgacccgtga tgtcgacggc
     2161 ttctactgcc gtgaagttgc gaaacgaatg tttgacgtgt ggtgccactg ccgtggatga
     2221 aagttttatg aggttggcat gcagacaatc atctatcaga taacccccag caaatggtgt
     2281 acggagagag tccttattgc atcaacaggg ctaaagcccg gcaccatcga gcgggccaga
     2341 agaaagtcat ggatgcaggg aaaagaatac cgccattacg ctgtagaagg tgatcctggg
     2401 cattacagtg aatgcctgta caacatcgaa gaaattatgc gatggatcga aaaccagaaa
     2461 caaccaggtg ccaaaaatgc aagttccggt taacctgtta atgctcctgg acgtctggga
     2521 ggtttaatga gtaacgcatc atacccgaca ggcgttgaaa accatggagg atcactccgt
     2581 atatggtttc actataatgg caaacgtgtc agagaaaacc tcggtgttcc tgacacagac
     2641 aaaaaccgga agatcgctgg tgaacttcgc acttccgttt gttttgcaat cagaatgggg
     2701 agtttcgact acgccgcgca gttccctaat tcccctaacc tgaaacactt tggtctggga
     2761 aaaagagaga taaccgttaa ggcactttcg gaaaaaatgg ttggacctta agaaaattga
     2821 gatttgtgcg aatgcactta accgttacca gtcagtaatt aaaaacatgt taccaatgtt
     2881 aggtgaaaaa aaactggttt catccataac aaaagaggat ttacttttcg taaggagaga
     2941 tttgttgacc ggttaccaaa agctttctaa tggaaagact tcttccataa aagggcgctc
     3001 agtggtcacg gtaaactact atatgacaac catagctgga atgtttcaat ttgcaacaga
     3061 taatggttat acctcaggaa acccatttaa cggtctggct cccttaaaaa agtccaaggt
     3121 aaaaccagat cctctcaccc gtgacgaatt tattcgtttt attgaggctt gccgtcatca
     3181 acaaacaaaa aacctgtgga ttctcgctgt atacacgggt attcgtcacg gggagttggt
     3241 atcgctggca tgggaagata tagaccttaa agcaaggact ataaccatcc gtagaaatta
     3301 tacaaaactt ggcgaattca ctccaccaaa aaccgatgca ggcaccggaa ggacaattca
     3361 tctggttcaa ccagctattg atgctcttaa aagccaggcg gaaatgacca tgcttggaaa
     3421 gcaacattct gtagaggtga agcagaggga atatgggaga actgctgtgc ataaatgcac
     3481 ttttgttttt agtcctcagg taacaaaaca gcagcagttg tccggacctc actacaaggt
     3541 tgactccatc agggagtcat ggacaagtat cttaaaacgc gcaggtctga gacacagaaa
     3601 atcgtaccaa tccaggcata cttatgcatg ctggtcactt gccgcaggag ctaatcctag
     3661 ttttatcgca agccagatgg gccacacaaa cgcacaaatg gtattcaatg tttacggagc
     3721 atggatgaaa gacaacaatc acgaacagat agaactcctt aacaaaagac tatctgaaag
     3781 tgtcccatgt atgccccata agaaagcagg gtaaaataaa aacttgcaaa atcaattagt
     3841 ttacccttaa tccctgtcac gttacgcgcg tggcagaggc gttacgggtt gctgaaaccg
     3901 caacggacag acggcggtca tcgactgttc aacgatgccg atattgaccg tatccgcgag
     3961 atcaaacgct ggatcgacaa cggcgtgcag gtcagcaaag ttaaaatgct gctcagtaat
     4021 gaaaatgttg atgtgcagaa cggctggcgc gatcagcaag aaacattact gacctacctg
     4081 caaagcggca atctgcatag cctgcgaacg tggatcaaag agcgcggtca ggattacccc
     4141 gcccagacac tcaccacaca tctgtttatt cctctgcgcc gacgacttca atgccaacaa
     4201 ccgactctcc aggcgctgct ggcgatcctc gacggcgtac tgatcaacta catcgccatt
     4261 tgtctggctt cggcacgtaa aaaacagggt aaagatgcgc tggtggttgg ctggaatatt
     4321 caggatacca cccgtctgtg gctggagggc tggattgcca gtcaacaagg atggcgcatt
     4381 gatgtcctcg cccactcgct caatcaacta cgccctgaac tattcgaagg tcgtacattg
     4441 ctggtgtggt gcggtgaaaa tcgaacctcc gcccaacagc agcaactcac cagttggcaa
     4501 gaacaaggcc atgatatttt cccactcggc atttaatgat tcgttaacaa atgcgcttta
     4561 ctgtacaatc ctttcgttaa cataaggagt gcattatgcg catagctaaa attggggtca
     4621 tcgccctgtt cctgtttatg gcgttcggcg gaattggtgg cgtcatgctc gcaggttata
     4681 cctttatttt gcgtgctggc taagcgcctg caccagcctt tcaaacaggc ggtctgcgat
     4741 gatcgccgcc agtgccacca gtaacgcccc ctggatcaca tacgcggtat taaatccgct
     4801 aagcccgatg atgatgggcg tacccagcgt gctggcccct accgttgagg cgatcgtcgc
     4861 cgtaccaatg ttgataatca ccgaagttcg cacgcccgcc agaatcaccg gagccgccag
     4921 cggtagctcg accttacgca gtcgctgacc acgactcatt cccatacctt tcgcaacttc
     4981 tgtcacgctg gcatcgatcg ctcccagccc ggcaagtgtc gcctgcagga cgggcagcac
     5041 accgtaaagg atcaaggcga taatcgctgg ttgcagacca aagccgatca ccggaacggc
     5101 aatcgccagc actgcgacag gcggaaaagt ctgtccaacg gcggcaatag tttccaccag
     5161 tgggcgaaat tccgcgcccc acgggcgagt gacagcaatt ccggcaccag tgccaatgat
     5221 caccgcaaac aaactcgaaa ttcccaccag ccagaaatga gccagtgcca gagctgcaaa
     5281 actttcttgc tgataaacgg gtcgtggcag ttgtgggaac aaggcagcaa acagcggctg
     5341 gctgtaaggc agccagaaaa tcagtgccac aaacaaagca atgagccaga acagcggatc
     5401 gcgcaacatc ttcatacgct tacgcctcca ccagcagatc ctgaaaatgc agcgtaccgc
     5461 aaggctggcc ctgcgtgttc accaccggca gcacctcgca accccgcgcg acaaacaggg
     5521 agagcgcatc gcgtagcgtc atctcttctg ccagtgcctc accttctgcc cgttcttcgc
     5581 gacgcacgta atccgccaca ctacgtaacg aaagcagacg cacacccagt tcgctacgtc
     5641 caaaaaactg gcggacaaaa tcattcgccg gacgagtcag catcgtcagc ggattgccct
     5701 gctgcaccac ttcaccgtga tccatcaata ccagatgttc agccagccgt agcgcctcat
     5761 caatatcatg agtgaccagc acaatagtgc gccccagcaa acggtgaatg cgcgtcatct
     5821 cttgttgcaa cgcgccgcgc gttaccgggt ccagtgcgcc aaaaggttca tccatcagta
     5881 agacttgcgg atcggcagcc agtgcgcgcg ccactcccac acgttgctgc tgaccaccgg
     5941 aaagctgatg cggataacgc tcacgcaaat ttgactccag ccccagtagc gtcattaatt
     6001 cgtcgatacg atcgtcaatc cgtgcccgcg accatttttg taattgcggc acggtagcga
     6061 tgttttgcgc cacgctccag tgggggaata ggccaataga ttgaatggca tagcccatcc
     6121 ggcggcgcaa ctccagtact ggcagcgagc gaatttcttc tccggcaaag cggatctctc
     6181 cgctgtcatg ttccaccagg cggttaatca ttttcaaggt ggtggatttg ccggagccag
     6241 atgtgccaat cagcaccgaa aaactccctt cctgaaaatt gagattaaga tcgttaacgg
     6301 ctttttgtgc gccaaacagt ttgctgacat ggctaaattc aatcattacg tttcaccttc
     6361 agcagtgcga taagcaaatc gaacagggca tcgatcagca ccgccagaac aattaccggg
     6421 atcaccccca gcaacactaa atcaatggcg ctgcttagca gcccctggaa aaccagcgca
     6481 ccaaaaccgc ctgcgccgat taacgccgca atcaccgcca tacctacagt ttgcaccatc
     6541 accacccgca ggctgcgcag aaataccggt aacgccagtg gtaactgaac atgcaggaat
     6601 cgctgcgccc cgctcatgcc catcgctctg gcgctctcca gcacatcgcg cgggatctgg
     6661 ttcaaaccga ctaccacgcc gcgcaccagc ggcagcaagg catagagcac cagcgcaatc
     6721 agtgcgggtg tcattccggt tcctgctatg ccgagcttcc ccagccacgg aaaggccgtc
     6781 accagcgcgg caagcggcgc aatcaacagg ccaaagagcg ccaccgaagg cacggtctga
     6841 atgacattga gcagagaaaa aattgccccc tgccgagcag tggaaaagta gcaccagatg
     6901 cccaacggca caccaatcac taacgcaggc agcaccgcac caaacagcaa cgtcagatgt
     6961 tgtgccagcg cgtcgtcaaa cacatcctga cggttggcgt attctttcat tagtgagaga
     7021 tcgttaagcg tgccggagta cagcaaccac agcggaataa tggcaatctg catatgcaac
     7081 aaccagcgcc acagcggatg cgtggagatt cggcggatgg catcgctaca ggccagcaat
     7141 gccagcgccg cagccagcca gaaaccactg ccgaggctgg tacgcgccag cgcactgcca
     7201 ttttgcgcca gttgtgtcgc cgcctttccc gcgccccaca ccagcaatac gaagacgaat
     7261 tgcgccagaa tgagtgcaca aatgctgcct tttttaccgg gaataaaaca ggccgtcagc
     7321 caggcgcaac caacgcccac cagcatccag atcgtttgcg gccacagttg ccagagatga
     7381 cgcccttcac ccgagactaa acgattaggc gcgtagctga taaacggcag cgctgctgcg
     7441 attgccgtca gcaacagcag cagcgccaga acaggattaa tacgtagata agtcacggga
     7501 aattacttcg cccacccttt ttgtttcagg tagtcggcag ccactttttt ggcatccagt
     7561 ccttccactg caatgctggc attcagttgc tgcaatgttt ttgcatcgag gctggcgaag
     7621 actggctgta gccactgtgc catttgcgga tactccttca acaccgattc acgcaccact
     7681 ggtgcaggcg catagatagg ttgcacgcct tgcggatcgc ttaaggtttg cagccccagc
     7741 gccgctaccg agccgtcagt gccgtaagcc attgcagcat taacgccaga ggtttgctgg
     7801 gcagcggctt tgatcgtcac cgccgtgtcg ccaccagcca gtgacagcaa ctgatcctga
     7861 ccgagcttaa agccataggc tttttcaaac gcgggtaacg catcggcgcg ttcgataaac
     7921 tctgccgagg ctgccagttt gaaggtgccg ccctcttgca gataacgact caggtcggca
     7981 agcgaagtga gtttattttt ctctgccaca tcctgacgca cggcgatagt ccaggtgtta
     8041 tttgcaggcg cgggcgtcag ccagattaac ttgttgtgct ccgaatcgag ctttttgact
     8101 ttctcgtaac cttgctgcgc gtttttccac gctgcatcgt tttcatcttt aaagaagaaa
     8161 gcgccattgc cggtatattc cggatagata tccagttcac cggaagtaat cgccccgcgc
     8221 accacaggag tcgttccgag ttgcacttta ttcaccgtcg gtacgccgtg gctttccagc
     8281 acctgcaaaa tgatattgcc gagtagcgca ccttcggtat cgatttttga accgacttta
     8341 acgggggaag ccgcctgtag cggcaggctc acggctgcca acataaccag tgaacctgcc
     8401 cagagcttta agagtggcat gatgctttcc tcattctttt actgttgttt tcagcgaatt
     8461 aagagaaaag catagttgat aatggcgggg ttagcttgag cggaaggatt cagttgcaga
     8521 atcagataaa tacttaagag gcatattcgg tccggcgttt cctgccggat gcggcgcgag
     8581 cgccttatcc ggcctacaaa gggcgcaaac gtcgcgccct tactaaagca ttacagcaac
     8641 tcaaactcgc ctttcttaac gcgtgcggaa tcagtgccga taaatacatt gaacttgcca
     8701 ggctcggcgt catatttcat ctgttgattc cagaacttca gcgcctcaat atcgatcggg
     8761 aagctgacag tctgagtttc gcccggtttc agggtgattt tctcaaagcc tttcagctgt
     8821 ttcacagggc gactcatgga agccgtcaca tcctgcaagt acatctgcac taccgtggcc
     8881 ccctcgcgct taccggtgtt cgtcacctgc acgctggcag tcactttgcc gtcacgcttc
     8941 atggtcggcg cagaaagttt cacatcagag acggtgaaag tggtgtagct cagcccatag
     9001 ccgaacggat acaacgcccc gttagcttca tcaaaataac gcgaagtgta tttgttcggc
     9061 ttgtcggcat tatacgggcg accggtattc agatggctgt agtacaccgg gatctgcccg
     9121 acagaacgcg ggaaggacat tggcagcttg ccggacgggt tgtaatcgcc aaacaataca
     9181 tcggcaattg cattaccgcc ttcagtcccc gcaaaccagg tttccagaat cgcatcagcc
     9241 tgctgatctt ctttcaccag cgccagcgga cgcccgttca tcagcaccag caccagcggt
     9301 ttaccggtgg ctttcagcgc cgcaatcaag tcacgttggc tttgcggaat agtgatatcg
     9361 gtccggctgg aggcttcgtg cgccatcccc tgtgcttcac cgactacagc caccaccaca
     9421 tcagattgtt tcgccgtctg caccgcttca tcaatcatct cttgcggcga acgcggatcg
     9481 actttgaccg cttcttcata ctgattcagg aaatcgataa tgcctttgtc actggtaacg
     9541 ttcgcccctt tggcatacag cactttaccg ttttcaccga cggaattttt aatcccggtc
     9601 agtacggtca cggattgatc ggcaacaccg gctgcggacc agctgcccat cacgtcacgt
     9661 ttactgtccg ccagtggccc aaccaccgca atggtggccg attttttcag cggtaacgtt
     9721 tcgagacggt ttttcagcaa caccaggctt tcgcgcgcca cttcacgcgc ttctttacgg
     9781 tgcaggcggc tttcggcatt ggtatccacc gggtcagact ctttcggccc caaatggctg
     9841 tatgggtcgt taaacaatcc catatcatat ttaacgttca gtacatggcg ggcagcatcg
     9901 tccagctctt ccatcgtcac tttgccggat ttgatcaacc caggcagata cttcgagtaa
     9961 tactcgtcgc tcatactcat gttgattccg gatttcagcg ccacgcgcac cgcatcttcc
    10021 gggtctgccg ccgtgccatg tttaatcagc tctttgattg caccgtgatc ggaaacggtg
    10081 atgcctttaa agccccactg gtcgcgcaga acatctttca gcagccagga atcggaggtg
    10141 gctggcgtgc cgttcagcga gttcagcgcc accatcaccg cgccgctgcc tgcgtccagc
    10201 cccgctttgt acggcggcat ataatcatta aacaggcgct gcggactcat atcgacggtg
    10261 ttgtactctt taccgccttc taccgcgccg tatgcggcaa agtgtttgac gctggtcatc
    10321 accgagtagc gatctgccgg gcttttaccc tgcatcgctt ccaccatggt tttacccatt
    10381 gttgaggtga gatacgtatc ttcgccaaaa ccttcggaag cacgtcccca gcgcggatcg
    10441 cgcgagacat cgaccatcgg tgcccaggtc atattcaggc catcatctgc cgcttcataa
    10501 gcagagacac gtccgaccgt tttcactgca tcgaggttaa aagacgaggc cagaccgagg
    10561 ctaatcggga acaccgtgcg ctgaccgtgc agcacgtcgt aagcaaagaa aagaggaatt
    10621 ttcaggcggc ttaattccat cacctgatcc tgcatggcgc ggatatcctg acgggttacg
    10681 gtgttgaaaa tcgccccaac ctgaccgtct ttgatcatct cgcggatcgc ctctttcggg
    10741 ttatccgggc cgacgctgat taagcgcagc tgaccaattt tctcatcaac tgtcattttc
    10801 ttaagcagtt cggtgacgaa cgcatcccgc gcttcgggcg ttaatggatg gttgccgaat
    10861 aaatcatccg ccagtgctgg ctgcagggcc agactcaccg cgattcctac tgaacatagc
    10921 catttcatat ggatttttct tcctctgttg cccgacgagg cagcgaaaat ggtgcaaaaa
    10981 ccgtagtttg ccataagcat gatggagaga gaaaaagaat gctcagttta ttgtctgaat
    11041 tttcaaaata ttcactcgct gaattgtcat acaaggcgct attctagttt gtgatatttt
    11101 ttcgccacca caaggagtgg aaaatgtctt ccatgacaac aactgataat aaagcctttt
    11161 tgaatgaact tgcccgtctg gtcggtcatt cacacctgct caccgatccc gcaaaaacgg
    11221 cccgctatcg caagggcttc cgttctggtc agggcgacgc gctggctgtc gttttccctg
    11281 gctcactact ggaattgtgg cgggtgctga aagcctgtgt caccgccgac aaaattattc
    11341 tgatgcaggc tgccaataca ggcctgaccg aaggatcgac gccaaacggt aacgactatg
    11401 atcgcgatat cgtgatcatc agcaccctgc gtctcgacaa gctgcacgtt cttggcaagg
    11461 gcgaacaggt gctggcctat ccgggcacca cgctctattc gctggaaaaa gccctcaaac
    11521 cgctgggacg cgaaccgcac tcggtgattg gatcgtcgtg tataggcgca tcggttatcg
    11581 gcggtatttg taacaactcc ggcggctcgc tggtgcaacg tggcccggcg tataccgaaa
    11641 tgtcgttatt cgcgcgtata aatgaagacg gcaaactgac gctggtgaac catctgggga
    11701 ttgatctggg cgaaacgccg gagcagatcc ttagcaagct ggatgatgat cgcatcaaag
    11761 atgacgatgt gcgtcacgat ggtcgtcacg cccacgatta tgactatgtc caccgcgttc
    11821 gtgatattga agccgacacg cccgcacgtt ataacgccga tccggatcgg ttatttgaat
    11881 cttctggttg cgccgggaag ctggccgtct ttgcggtacg tctagatacc ttcgaagcgg
    11941 aaaaaaatca gcaggtgttt tatatcggca ccaaccagcc ggaagtgctg accgaaatcc
    12001 gccgtcatat tctggctaac ttcgaaaatc tgccggttgc cggggaatat atgcaccggg
    12061 atatctacga tattgcggaa aaatacggca aagacacctt cctgatgatt gataagttag
    12121 gcaccgacaa gatgccgttc ttctttaatc tcaagggacg caccgatgcg atgctggaga
    12181 aagtgaaatt cttccgtccg cattttactg accgtgcgat gcaaaaattc ggtcacctgt
    12241 tccccagcca tttaccgccg cgcatgaaaa actggcgcga taaatacgag catcatctgc
    12301 tgttaaaaat ggcgggcgat ggcgtgggcg aagccaaatc gtggttggtg gattatttca
    12361 aacaggccga aggcgatttc tttgtctgta cgccggagga aggcagcaaa gcgtttttac
    12421 accgtttcgc cgctgcgggc gcagcaattc gttatcaggc ggtgcattcc gatgaagtcg
    12481 aagacattct ggcgttggat atcgctctgc ggcgtaacga caccgagtgg tatgagcatt
    12541 taccgccgga gatcgacagc cagctggtgc acaagctcta ttacggccat tttatgtgct
    12601 atgtcttcca tcaggattac atagtgaaaa aaggcgtgga tgtgcatgcg ttaaaagaac
    12661 agatgctgga actgctacag cagcgcggcg cgcaataccc tgccgagcat aacgtcggtc
    12721 atttgtataa agcaccggag acgttgcaga agttctatcg cgagaacgat ccgaccaaca
    12781 gcatgaatcc ggggatcggt aaaaccagta aacggaaaaa ctggcaggaa gtggagtaaa
    12841 aattacggat ggcagagtat cgccatccga attcacttaa tcgttctgtg ccgtctgccc
    12901 cgccgccgcc atttgggcgg ctttttgttt tttatagctc aacgctgctg ccggcacagg
    12961 catcacttta ccggtttcaa tccaggtacg caggcggctg gcatcggcaa aatgggtata
    13021 tttgccaaac gcgtccatca ctaccagcgc caccggttta ttattgataa ccgtacgcat
    13081 caccagacaa tggcccgccg cattggtaaa gccggttttg gttaactgaa tattccagtt
    13141 atcgcgatac accagatgat tagtattgcg gaacggcagc gtatacgtcg ggttagagaa
    13201 ggttgccata tcttcccggg tagtacttaa ctgcccgatc aacggatact gtttgctggc
    13261 aatgagcagt ttggttaagt cacgggcagt tgaaacgtta tgcaccgaca atccggtagg
    13321 ttcaacaaag cgcgtgttgt tcattccgag cgatttcgct ttcgcattca ttgccttaat
    13381 aaaggctttg taaccaccgg gataatggtg cgcaaggctt gccgccgcgc ggttttctga
    13441 agacatcagc gccagcaaca gcatatcttt acggctgatt tcgctattca gtcgtacgcg
    13501 cgaatagacc cctttcatct ccggcgtctg gctgatatcc acttttagtt tttcatccag
    13561 cggcagtcgt gcatccagca caaccatcgc ggtcattaat ttgctgatag acgcaatcgg
    13621 acgcaccaga tccgggtggt tcgaatagat cactttgttg gtattcagat caacaatcat
    13681 cgcgctaccg gaggcaattt ccggttgtga agcggtggta gcggctgccg ttttcgcaac
    13741 ggcctgcggt gccaaaggca cagccagcat cagggccagg ctaaataaag aaactcgaaa
    13801 tttcggcatg atgagcattc agatagtggt tcacgcgcac gggttgcgca ccgccggagt
    13861 aaggatttac tgaggctagc gacgccatca taacgagcaa aaagtgcgat cgtcaaagga
    13921 gaatcgtgag gaaatgctgc attgctgaca tttacgccag caatgcaatg tcaaagaact
    13981 ttctagaaca ggcgataccc gtagccccat agtataacgg ttagggcgag cagcacttcc
    14041 agtaccagca cgccaatcgc cagcgtcgaa ctggagaagc taaggccttc ctctttgttg
    14101 atattcagga agctcggaat accaaggtaa agcaggtagc cggtgtaaaa cagcgccacc
    14161 gtgccgacca gcgcacacaa ccagaccagc ggataaagcg ccaccagacc gcttaaaaac
    14221 agcggagttg caacgtagcc cgcgaagacc atgcagtgcg caagtgacgg acgctgcggg
    14281 taattgcgcg ccatccacca gatgacccgc cccatcaccg caaccccagc gagcataacg
    14341 ccataaaaca ggacagccag cgccagtccg gtaaaccagg acaacttcag gatagtgcca
    14401 tcgccaaaat tccagccaat ctgtgtagtg ccaatgaagg cgcaaatcac cggaatcgcc
    14461 gccatcagca aaacgtggtg ggtgtaatgg tgagaaatcg tttcgttttc gcgattaatc
    14521 acctgcattt cacgatcggg atgggaaaac agtccccaga catggctcat accgccccct
    14581 tgttgtgagt tcatgaacct gacagttcaa gtataagtca gcttgtgatt attttttgtt
    14641 cgctcatgta atttgcaccg tctgatgtac gtcctttttc agcgtgaatg atttccaggg
    14701 tgtatgctta caggcaacca aagggagaca gactggccta tggatctcaa tacactcatc
    14761 tcacaatatg gttatgccgc gctggtgatc ggtagcctgg cggaaggtga aaccgtgact
    14821 ttgctgggag gcgttgcggc gcatcagggg ctattaaagt tcccgctggt ggtactttct
    14881 gtggcgcttg gcggcatgat tggcgaccag gtgctttatc tgtgcgggcg gcggtttggc
    14941 ggcaagctgt tacgccgttt ctcgaaacat caggataaaa ttgagcgggc gcagaaactt
    15001 atccaacgcc atccgtatct gtttgtcatt ggtacgcgct ttatgtatgg ctttcgggtg
    15061 attggcccga cgctgattgg tgccagccag ctgccgccga aaatctttct gccgcttaat
    15121 attctcggcg catttgcctg ggcgttgatt tttaccacta tcggttacgc tggtggtcag
    15181 gtgattgcgc cgtggttgca caatctcgat cagcatttga agcactgggt ctggttgatt
    15241 ctggtcgtgg ttctggtggt aggcgtgcgc tggtggctga aacgacgcgg gaagaaaaag
    15301 ccggataatc aggcgtaaaa ccattgccct ggataaggcg ttcgcgccgc atccgacatc
    15361 cggtgcacca tgcctgatgc ggcgcttgtg cgtcttatca tgcctaccaa aattttaccc
    15421 attgaaatat ccacacacct cactgcgcta ttctgggttg aactgcggat tcgccaacat
    15481 aaagccgcca tccactatca acgactgccc ggtggtgtaa tttgcgccct ccgaacaaag
    15541 ccacgccacc aggctggcaa tctcatacgt tgcgccaaaa cgccgcagag gaatcgaagg
    15601 ctccgcgtcg ggcttcacgt cgctatcatc catgccgttc attggcgtag cgatcgcccc
    15661 aggtgcgact gcgttcacca aaatcttatg cctgaccaac tccagcgcca ttgctttggt
    15721 taacccgccg agcgcatgtt tagcggctgt gtaggcgctg gcatccggca gcggcgtatg
    15781 ttcatgtacc gacgtaatgt tgatgatgcg accaccctgc ccttgtttca ccatctggcg
    15841 ggccgcaatt tgtgagcata aaaatgcacc atcgacatca acggtaaaaa tcttgcgcca
    15901 ctcatcgaaa gccatatcaa gaaacggcgc tttggtcatt gcgcccgcat tattcaccag
    15961 cacatcaata cgccccagcc gttgaatgag tttctccagc gcctgtgcac cttctggcag
    16021 tttaccgaga tccagctgca caatctccgc acgtacgccg tggctaacta cctcacgcgc
    16081 ggtatctttt gccccttctt catctgagtg ccaggtaatg ccaatatcaa acccctgctg
    16141 cgccagtaat aacgcgcact ctttgccgat ccccgaatcg gaggcggtaa taatcgcaac
    16201 ctgtgccatc gagttctcca cttaacgctg aataaacgtt aagtatagaa ggcgcatatc
    16261 atcagcgttt gtaacccccg cccaacgcac cagtgagttg aatggaagca tccagccact
    16321 gcccttgcaa taacaggcca ttggcccgct cacgcagcgc ggggattctg gcttcgctga
    16381 cgcgggaacc agcaatgatg cccgcgttaa agcgcgcctg cgcaagacct accacacgca
    16441 aggcatcgcg ctcaatttgc gcctgatgct ggtttttctc cgcaagtgtc tgaacctgac
    16501 tggctgcccg cgccacgtca ttcaccgctt caaccaccgc tttgttgtag ctggcgatag
    16561 acaagttgct ttcggctttt gcgatatcga gattggcgtt aagacgacca ctatcgaaaa
    16621 tgggtagcgt caggcctgcc gtaacgccca tttgctgcgc ggaatgacgg aacagatcgc
    16681 ttaagtgcaa cgcatcctgt tgcaggaagg ccatcaggtt gatgtcagga taaaatgccg
    16741 cttttgccgc atcaatggtg cttagcgatg actcaacgta ccagtgcgcc gcctgcaaat
    16801 ctgcccgccg ggccagtaag gagtacccca gttcatcagg aagctggctt gccactttcg
    16861 gcaacgcgac cggtttaagc ttcaatgact ttgtctggtt atttgtaagt gcgcttaacc
    16921 gtgcctcaat aattttcatt ttccccgcga catcgttgag ctgctgccgg gttttgctgg
    16981 cattaatatc ggtttccaca ccttcaactg aagaagtaat cccgttctga tatagctggc
    17041 gatcggtcgc gataatggtg ttctgctctt tttctatttg ctgcaagacc gtgtttaacg
    17101 ccgcctgggt ttgccactcc cagtacaggc gggctacgct gccagccagc aattggcggg
    17161 tttgctcgcg ttccgccgcc cgtgctttaa ccgtacccag gcgggcagta acctccgccc
    17221 gattctttcc ccagatatcg agatgccagc ccgccgttaa gccaaaagtt ccgttggtgt
    17281 accacggacc ggtcgtacct gcggccggat cgttcagagc aaacggcccc attaagcctt
    17341 ctgccgacat tttttgccgc tccatatccg cagaaaagtc gatctgcgga ccatcctgag
    17401 tagcaattgc cttcgcctgg gcttcagcta gctgaatgcg ctgttcagcc acctgcatat
    17461 ccggtgcgtt ctgtagtgca ttgttaatta aggaagtgag ttgattatcg tgatactcca
    17521 gccaccattg gctgtctggc caaccatttt tcagcgccgt gggtaatgcg gtgtcaactt
    17581 gtgcagcggg cgtttgctgg cttaacgcct ggcgggtttc atgcataggc gcacacccgg
    17641 ccagcatcag taacagcgga aaacaggcga tggcgggata aaaggaatca cgattcatgg
    17701 gggaataatc aggtaagaaa aggtgcgcgg agattaccgt gtgttgcgat atatttttta
    17761 gtttcgcgtg gcaatacatc agtagcaata aaacgacata tccagaaaaa tatacactaa
    17821 gtgaatgata tcttccgatt tatcttaatc gtttatggat aacggcaaag ggcttcgttt
    17881 tttcctatac ttattcagca ctcacaaata aaggaacgcc aatgaaaatt atactctggg
    17941 ctgtattgat tattttcctg attgggctac tggtggtgac tggcgtattt aagatgatat
    18001 tttaaaaatc agatatggca tataagttgt caggtcagga actagatatt ttgtgtactt
    18061 gatttgcaaa cagctccggt gttggtggcg tcaaaatcgc tgaagcgttc cccgaaggcc
    18121 ggggcagccc acatggatgt gggctgaggg cgcgtcttac aaggacgtta cctcgcgccc
    18181 gacccggtag acgaagggga taagctgaag gtaccgcgca gcggcgattt tgttcgccag
    18241 agcccggggg tgcatggggc ggcggcgatt ggccgccccc tgcgcgctcc ttgcgccagt
    18301 ggcaatatgt ggcttagctc atgaaaggag cgcaacaaga tgatgaagct gaataagcga
    18361 ttcattgtct ctgataaact gcgattccac tattatcgca gcttatcgat atcaattgcc
    18421 tgaatagccc ttgcaatatc aggggaatta ttcaacaccc gaacatgctg aaataattcc
    18481 gttgcttcat cgtattcttt acgcaaataa ctcaaccact gtttaatccg cgcaacgtga
    18541 tataacccgg tatcgccctg cttttccaga cgggtatatt tttgcagcaa agcaaccacc
    18601 tccggccacg gcattcgcgg ttcgttatat tttactaccc ggctcaggtt gggaatattg
    18661 agcgccccgc gaccaatcat cactgcgtcg cagccgctga tcgcaatgca ttgttgcgcg
    18721 ctctgccagt cccagatttc gccgttggca atcaccggaa tattcagccg ctggcgaatc
    18781 tcgccaatcg cctgccagtc aatatgctcc gcgcggtaac cctgctcttt tgtccgtcca
    18841 tgcaccacca gctccgtagc accagcctgt tgaaccgcat cggcgatttc aaatttcttc
    18901 tcaccgctgt cccagcccag acgcactttc acgctgacgg gcaaatgcgc cggtacagct
    18961 tcacgcatcg cttttgcacc ctggtagatg agttcaggat ctttgagtaa cgtcgcccca
    19021 ccaccgctac cgttgaccgt tttcgacggg cagccgcaat tgagatccac gccccaggaa
    19081 cctaattcca ccgcacgggc ggcgttctct gccagccatt gtgggaactg acccaacaac
    19141 tgcacgcgca ccagcgtacc ggatggtgtc cggctggcgt tttgtagctc agggcaaatg
    19201 cgatgaaaaa cttttaccgg cagcagttga tccaccacgc ggacaaactc ggtgatgcac
    19261 agatcgtagt cgttaacttc ggtcagcaat tcacgcacca gagagtcaag cacaccctcc
    19321 atcggagcca gtaacacacg catatcttca cccgcaaaaa aatgaggcgc tatgttagcg
    19381 cctatggtca acggattaaa ggtctgcaaa gagggattgc tgcaaactgt aatccctctc
    19441 tatgcttgat gtatgcctga gttcgttgcg cattgcgtaa tgccggggag cgaaaaaata
    19501 agcgctgatt tcttaatgtg atcggtagca cgttttacga attaattgta tgatgaatcc
    19561 atctcatctg gggtgttgat tatgagtaag acactgaaca ttatctggca atatttacgc
    19621 gctttcgtcc tgatttatgc ctgcctgtat gcaggcattt tcattgcttc cctgctaccg
    19681 gtaaccattc cgggcagcat catcggtatg ctgatcctgt ttgtcctgct ggccttgcaa
    19741 attcttccgg caaaatgggt caatccgggg tgctacgtac tgattcgcta tatggcgcta
    19801 ttgtttgtgc cgattggcgt aggcgtcatg caatattttg atttgctccg cgcacagttt
    19861 ggcccggtag tggtttcctg tgcaatcagt acgctggtgg tttttctggt ggtgagctgg
    19921 agttcgcaac tggtacacgg tgaacgtaaa gtcgtaggtc agaaaggatc agaagaatga
    19981 tggcaaatat ctggtggtca ttaccgctga cgttgattgt cttttttgcc gcccgcaaac
    20041 tggcggcacg gtataagttt cccctgctta acccgttgct ggtagcaatg gtggtgatca
    20101 ttcctttttt aatgctgacc ggcatctctt acgacagcta ctttaaaggt agcgaagtgc
    20161 taaacgatct gctgcaaccg gcggtcgtcg cgctggccta tcctttatat gagcagctac
    20221 accagatccg cgcgcgctgg aaatcgatca tcaccatctg ttttatcggc agcgtggttg
    20281 cgatggtgac ggggacttcc gtggcattgt tgatgggcgc ttcaccggaa atcgccgcgt
    20341 caatcctgcc gaaatcagtc accacgccta ttgcaatggc ggttggcggc agtattggcg
    20401 gtattccggc aatcagcgca gtttgcgtga ttttcgtcgg catcctcggc gcggtatttg
    20461 gccatacatt gcttaatgcg atgcgtattc gtaccaaagc tgcgcgcggt ctggcaatgg
    20521 ggactgcctc gcacgccctc ggtacggcgc gctgcgccga gctggattat caggaaggtg
    20581 catttagttc gctagcgctg gtgttatgcg ggataattac ttcgctgatc gcaccgttcc
    20641 ttttcccgat tattctggca gtaatgggct aaaatttgcg atgcgtcgcg catttttgat
    20701 gtaagtttca cgcgttgcat aattaatgag attcagatca catataaagc cataacgggt
    20761 tcgtaaactg ttatcccatt acatgattat gaggcaacgc catgcatcca cgttttcaaa
    20821 ccgcttttgc ccaacttgcg gataacttgc aatctgcact ggaacctatt ctggcagaca
    20881 agtacttccc cgctttgttg accggggagc aagtctcatc gctgaagagc gcaacggggc
    20941 tggacgaaga cgcgctggca ttcgcactac ttccgctggc ggcggcctgt gcgcgtacgc
    21001 cattgtcgaa ttttaatgtt ggcgcaattg cgcgcggtgt gagcggaacc tggtatttcg
    21061 gtgccaatat ggaatttatt ggtgcgacaa tgcagcaaac cgttcatgcc gaacaaagcg
    21121 cgatcagcca cgcctggttg agtggtgaaa aagcgcttgc agccatcacc gttaactaca
    21181 cgccttgtgg tcactgccgt cagtttatga atgaactgaa cagcggtctg gatctgcgta
    21241 ttcatctgcc gggccgcgag gcacacgcgc tgcgtgacta tctgccggat gcctttgggc
    21301 cgaaagatct ggagattaaa acgctgctga tggacgagca ggatcacggc tatgcgctga
    21361 ctggtgatgc gctttctcag gcggcgattg cagcggcaaa ccgttcgcac atgccttaca
    21421 gtaagtcgcc aagcggtgtc gcgctggaat gtaaagacgg tcgtattttc agtggcagct
    21481 acgctgaaaa cgccgcattc aacccgactc tgccaccgtt gcagggagcg ttaattctgt
    21541 tgaatctcaa gggttatgat tacccggata tccagcgcgc ggttctggca gaaaaagccg
    21601 atgcgccgtt gattcagtgg gatgccacct ccgcaacgct gaaagctctc ggctgtcaca
    21661 gtatcgaccg agtgcttctc gcttaagcct ggtgccggat gcggcgtgaa cgccttgtcc
    21721 ggcttgccag ccctctcctg gtgtcgaaat tcccggcaaa cagtttgccg tttcttgcgc
    21781 aaaaccagcg ggtaaagtag cctgatggaa attttcctta gatcgagtct cctgcatgtt
    21841 aaagcgcgtg ttcctcagcc tgttagtcct gatcggcttg ctgctgttga ctgtgctcgg
    21901 cctcgatcgc tggatgagct ggaaaaccgc gccttatatc tacgacgaat tgcaggatct
    21961 cccctaccgc caggtcggtg tggtgctcgg aacagcaaaa tattatcgta ctggcgtaat
    22021 taatcagtat tatcgctacc gcattcaagg agcgattaat gcctataaca gcggtaaggt
    22081 aaattatcta ttactgagcg gcgataacgc attgcaaagt tataatgagc cgatgaccat
    22141 gcgcaaagat ttaatcgctg ctggtgtcga cccatcagat attgttctcg attacgcagg
    22201 ctttcgtacg ctggattcca tcgtgcgtac acgcaaagtt ttcgatacta atgatttcat
    22261 tattatcacc caacgtttcc actgtgagcg ggcattattt attgcgctgc atatggggat
    22321 tcaggctcag tgttatgccg taccgtcacc gaaagatatg ctgtcagtac gtattcgcga
    22381 atttgccgcc cgtttcggtg cgctggctga cctttatatt tttaaacgtg aaccgcgttt
    22441 tttagggccg ctggtcccta ttccggctat gcaccaggtg ccggaagatg cgcaggggta
    22501 tcccgccgtc acacccgaac agttacttga attacaaaag aaacaaggaa agtagttatg
    22561 gatgtacagc agttttttgt cgttgccgtt tttttcctta ttccgatatt ttgtttccgc
    22621 gaagcatgga aaggctggcg cgcaggcgcg attgataaac gggttaaaaa tgcaccggaa
    22681 ccggtgtatg tctggcgagc aaaaaatccc ggactctttt tcgcttatat ggtggcatat
    22741 atcggcttcg gaattttatc tatcggcatg attgtttatc ttattttcta tcgttaat
//
LOCUS       DAZKHF020000056        22499 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2201S31241
            SAMN43267800-rid25106413.denovo.056, whole genome shotgun sequence.
ACCESSION   DAZKHF020000056 DAZKHF020000000
VERSION     DAZKHF020000056.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267800
            Sequence Read Archive: SRR30307263
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 22499)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22499)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 22499)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350581.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350581.2
            Genome Coverage       :: 151x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:21:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,652
            CDSs (total)                      :: 5,557
            Genes (coding)                    :: 5,320
            CDSs (with protein)               :: 5,320
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 237
            CDSs (without protein)            :: 237
            Pseudo Genes (ambiguous residues) :: 0 of 237
            Pseudo Genes (frameshifted)       :: 102 of 237
            Pseudo Genes (incomplete)         :: 130 of 237
            Pseudo Genes (internal stop)      :: 52 of 237
            Pseudo Genes (multiple problems)  :: 41 of 237
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22499
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267800-rid25106413.denovo.056"
                     /serotype="O 26:H11"
                     /isolate="2201S31241"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-01"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            40..2076
                     /gene="yihQ"
                     /locus_tag="ACHOSC_RS08720"
                     /old_locus_tag="pgaptmp_001744"
     CDS             40..2076
                     /gene="yihQ"
                     /locus_tag="ACHOSC_RS08720"
                     /old_locus_tag="pgaptmp_001744"
                     /EC_number="3.2.1.199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfoquinovosidase"
                     /protein_id="HGU4547702.1"
                     /translation="MDTPRPQLLDFQFHQNNDSFTLHFQQRLILTHSKDNPCLWIGSG
                     IADIDMFRGNFSIKDKLQEKIALTDAIVSQSPDGWLIHFSRGSDISATLNISADDQGR
                     LLLELQNDNLNHNRIWLRLAAQPEDHIYGCGEQFSYFDLRGKPFPLWTSEQGVGRNKQ
                     TYVTWQADCKENAGGDYYWTFFPQPTFVSTQKYYCHVDNSCYMNFDFSAPEYHELALW
                     EDKATLRFECADTYISLLEKLTALLGRQPELPDWIYDGVTLGIQGGTEVCQKKLDTMR
                     NAGVKVNGIWAQDWSGIRMTSFGKRVMWNWKWNSENYPQLDSRIKQWNQEGVQFLAYI
                     NPYVASDNDLCEEAAQHGYLAKDASGGDYLVEFGEFYGGVVDLTNPEAYAWFKEVIKK
                     NMIELGCGGWMADFGEYLPTDTYLHNGISAEIMHNAWPALWAKCNYEALEETGKLGEI
                     LFFMRAGSTGSQKYSTMMWAGDQNVDWSLDDGLASVVPAALSLAMTGHGLHHSDIGGY
                     TTLFEMKRSKELLLRWCDFSAFTPMMRTHEGNRPGDNWQFDGDAETIAHFARMTSVFT
                     TLKPYLKEAVALNAKSGLPVMRPLFLHYEDDAHTYTLKYQYLLGRDILVAPVHEEGRS
                     DWTLYLPEDNWVHAWTGEAFRGGEVTVNAPIGKPPVFYRADSEWAALFASLKSI"
     gene            2122..3507
                     /locus_tag="ACHOSC_RS08725"
                     /old_locus_tag="pgaptmp_001745"
     CDS             2122..3507
                     /locus_tag="ACHOSC_RS08725"
                     /old_locus_tag="pgaptmp_001745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HGU4547703.1"
                     /translation="MSHITTEDPATLRLPFKEKLSYGIGDLASNILLDIGTLYLLKFY
                     TDVLGLPGTYGGIIFLISKFFTAFTDMGTGIMLDSRRKIGPKGKFRPFILYASFPVTL
                     LAIANFVGTPFDVTGKTVMATILFMLYGLFFSMMNCSYGAMVPAITKNPNERASLAAW
                     RQGGATLGLLLCTVGFVPVMNLIEGNQQLGYIFAATLFSLFGLLFMWICYSGVKERYV
                     ETQPTNPAQKPGLLQSFRAIAGNRPLFILCIANLCTLGAFNVKLAIQVYYTQYVLNDP
                     ILLSYMGFFSMGCIFIGVFLMPGAVRRFGKKKVYIGGLLIWVLGDLLNYFFGGGSVSF
                     VAFSCLAFFGSAFVNSLNWALVSDTVEYGEWRTGVRSEGTVYTGFTFFRKVSQALAGF
                     FPGWMLTQIGYVPNVAQADHTIEGLRQLIFIYPSALAVVTIVAMGCFYSLNEKMYVRI
                     VEEIEARKRTA"
     gene            3550..4953
                     /locus_tag="ACHOSC_RS08730"
                     /old_locus_tag="pgaptmp_001746"
     CDS             3550..4953
                     /locus_tag="ACHOSC_RS08730"
                     /old_locus_tag="pgaptmp_001746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HGU4547704.1"
                     /translation="MSDHNPLTLKLNLREKIAYGMGDVGSNLMLCIGTLYLLKFYTDE
                     LGMPAYYGGIIFLVAKFFTAFTDMLTGFLLDSRKNIGPKGKFRPFILYAAVPAALIAT
                     LQFIATTFCLPVKTTIATALFMMFGLSYSLMNCSYGAMIPAITKNPNERAQLAAYRQG
                     GATIGLLICTVAFIPLQSLFSDSTVGYACAALMFSIGGFIFMMLCYRGVKEHYVDTAP
                     TGHKASILKSFCAIFRNPPLLVLCIANLCTLAAFNIKLAIQVYYTQYVLNDINLLSWM
                     GFFSMGCILVGVLLVPVTVKCFGKKQVYLAGMVLWAVGDILNYFWGSNSFTFVMFSCV
                     AFFGTAFVNSLNWALVPDTVDYGEWKTGIRAEGSVYTGYTFFRKISAALAGFLPGIML
                     TQIGYVPNIAQSDATLQGLRQLIFIWPCALAIIAALTMGFFYTLNEKRFALIIEEINQ
                     RKNKEMATEEKTASVTL"
     gene            5021..5713
                     /gene="ompL"
                     /locus_tag="ACHOSC_RS08735"
                     /old_locus_tag="pgaptmp_001747"
     CDS             5021..5713
                     /gene="ompL"
                     /locus_tag="ACHOSC_RS08735"
                     /old_locus_tag="pgaptmp_001747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porin OmpL"
                     /protein_id="HGU4547705.1"
                     /translation="MKKINAIILLSSLTSASVFAGAYVENREAYNLASDQGEVMLRVG
                     YNFDMGAGIMLTNTYTFQREDELKHGYNEIEGWYPLFKPTDKLTIQPGGLINDKSIGS
                     GGAVYLDVNYKFVPWFNLTVRNRYNHNNYSSTDLSGELDNNDTYEIGTYWNFKITDKF
                     SYTFEPHYFIRVNDFNSSNGKDHHWEITNTFRYRINEHWLPYFELRWLDRNVEPYHRE
                     QNQIRIGTKYFF"
     gene            complement(5804..7069)
                     /locus_tag="ACHOSC_RS08740"
                     /old_locus_tag="pgaptmp_001748"
     CDS             complement(5804..7069)
                     /locus_tag="ACHOSC_RS08740"
                     /old_locus_tag="pgaptmp_001748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HGU4547706.1"
                     /translation="MLTKKKWALFSLLTLCGGTIYKLPSLKDAFYIPMQEYFHLTNGQ
                     IGNAMSVNSFVTTVGFFLSIYFADKLPRRYTMSFSLIATGLLGVYLTTMPGYWGILFV
                     WALFGVTCDMMNWPVLLKSVSRLGNSEQQGRLFGFFETGRGIVDTVVAFSALAVFTWF
                     GSGLLGFKAGIWFYSLIVIAVGIIIFFVLNDKEEAPSVEVKKEDGASQNTSMTSVLRD
                     KTIWLIAFNVFFVYAVYCGLTFFIPFLKNIYLLPVALVGAYGIINQYCLKMIGGPIGG
                     MISDKILKSPSKYLCYTFIISTAALVLLIMLPHESMPVYLGMACTLGFGAIVFTQRAV
                     FFAPIGEAKIAENKTGAAMALGSFIGYAPAMFCFSLYGYILDLNPGIIGYKIVFGIMA
                     CFAFCGAVVSVMLVKRISQRKKEMLAAEA"
     gene            complement(7171..8151)
                     /locus_tag="ACHOSC_RS08745"
                     /old_locus_tag="pgaptmp_001749"
     CDS             complement(7171..8151)
                     /locus_tag="ACHOSC_RS08745"
                     /old_locus_tag="pgaptmp_001749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIM barrel protein"
                     /protein_id="HGU4547707.1"
                     /translation="MVTINNARKILQRVDTLPLYLHAYAFHLNMRLERVLPADLLDIA
                     SENNLRGVKIHVLDGERFSLGNMDDKELSAFGDKARRLNLDIHIETSASDKASIDEAV
                     AIALKTGASSVRFYPRYEGNLRDVLSIIANDIAYVRETYQDSGLTFTIEQHEDLKSHE
                     LVSLVKESEMESLSLLFDFANMINANEHPIDALKTMAPHITQVHIKDALIVKEPGGLG
                     HKACISGQGDMPFKALLTHLICLGDDEPQVTAYGLEEEVDYYAPAFRFEDEDDNPWIP
                     YRQMSETPLPENHLLDARLRKEKEDAINQINHVRNVLQQIKQEANHLLNH"
     gene            complement(8159..8869)
                     /locus_tag="ACHOSC_RS08750"
                     /old_locus_tag="pgaptmp_001750"
     CDS             complement(8159..8869)
                     /locus_tag="ACHOSC_RS08750"
                     /old_locus_tag="pgaptmp_001750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HGU4547708.1"
                     /translation="MAENQSTVENAKEKLDRWLKDGITTPGGKLPSERELGELLGIKR
                     MTLRQALLNLEAESKIFRKDRKGWFVTQPRFNYSPELSASFQRAAIEQGREPSWGFTE
                     KNRTSDIPKTLAPLIAVTPSTELYRITGWGALEGHKVFYHETYINPEVAPGFIEQLEN
                     HSFSAVWEKCYQKETVVKKLIFKPVRMPGDISKYLGGSAGMPAILIEKHRADQQGNIV
                     QIDIEYWRFEAVDLIINL"
     gene            complement(9086..10909)
                     /gene="typA"
                     /locus_tag="ACHOSC_RS08755"
                     /old_locus_tag="pgaptmp_001751"
     CDS             complement(9086..10909)
                     /gene="typA"
                     /locus_tag="ACHOSC_RS08755"
                     /old_locus_tag="pgaptmp_001751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-dependent GTPase TypA"
                     /protein_id="HGU4547709.1"
                     /translation="MIEKLRNIAIIAHVDHGKTTLVDKLLQQSGTFDSRAETQERVMD
                     SNDLEKERGITILAKNTAIKWNDYRINIVDTPGHADFGGEVERVMSMVDSVLLVVDAF
                     DGPMPQTRFVTKKAFAYGLKPIVVINKVDRPGARPDWVVDQVFDLFVNLDATDEQLDF
                     PIVYASALNGIAGLDHEDMAEDMTPLYQAIVDHVPAPDVDLDGPFQMQISQLDYNSYV
                     GVIGIGRIKRGKVKPNQQVTIIDSEGKTRNAKVGKVLGHLGLERIETDLAEAGDIVAI
                     TGLGELNISDTVCDTQNVEALPALSVDEPTVSMFFCVNTSPFCGKEGKFVTSRQILDR
                     LNKELVHNVALRVEETEDADAFRVSGRGELHLSVLIENMRREGFELAVSRPKVIFREI
                     DGRKQEPYENVTLDVEEQHQGSVMQALGERKGDLKNMNPDGKGRVRLDYVIPSRGLIG
                     FRSEFMTMTSGTGLLYSTFSHYDDVRPGEVGQRQNGVLISNGQGKAVAFALFGLQDRG
                     KLFLGHGAEVYEGQIIGIHSRSNDLTVNCLTGKKLTNMRASGTDEAVVLVPPIRMTLE
                     QALEFIDDDELVEVTPTSIRIRKRHLTENDRRRANRAPKDD"
     gene            11282..12691
                     /gene="glnA"
                     /locus_tag="ACHOSC_RS08760"
                     /old_locus_tag="pgaptmp_001752"
     CDS             11282..12691
                     /gene="glnA"
                     /locus_tag="ACHOSC_RS08760"
                     /old_locus_tag="pgaptmp_001752"
                     /EC_number="6.3.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020233234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004356 - glutamine synthetase activity
                     [Evidence IEA];
                     GO_process: GO:0006542 - glutamine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--ammonia ligase"
                     /protein_id="HGU4547710.1"
                     /translation="MSAEHVLTMLNEHEVKFVDLRFTDTKGKEQHVTIPAHQVNAEFF
                     EEGKMFDGSSIGGWKGINESDMVLMPDASTAVIDPFFADSTLIIRCDILEPGTLQGYD
                     RDPRSIAKRAEDYLRSTGIADTVLFGPEPEFFLFDDIRFGSSISGSHVAIDDIEGAWN
                     SSTQYEGGNKGHRPAVKGGYFPVPPVDSAQDIRSEMCLVMEQMGLVVEAHHHEVATAG
                     QNEVATRFNTMTKKADEIQIYKYVVHNVAHRFGKTATFMPKPMFGDNGSGMHCHMSLS
                     KNGVNLFAGDKYAGLSEQALYYIGGVIKHAKAINALANPTTNSYKRLVPGYEAPVMLA
                     YSARNRSASIRIPVVSSPKARRIEVRFPDPAANPYLCFAALLMAGLDGIKNKIHPGEA
                     MDKNLYDLPPEEAKEIPQVAGSLEEALNELDLDREFLKAGGVFTDEAIDAYIALRREE
                     DDRVRMTPHPVEFELYYSV"
     gene            13010..14059
                     /gene="glnL"
                     /locus_tag="ACHOSC_RS08765"
                     /old_locus_tag="pgaptmp_001753"
     CDS             13010..14059
                     /gene="glnL"
                     /locus_tag="ACHOSC_RS08765"
                     /old_locus_tag="pgaptmp_001753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrogen regulation protein NR(II)"
                     /protein_id="HGU4547711.1"
                     /translation="MATGTQPDAGQILNSLINSILLIDDNLAIHYANPAAQQLLAQSS
                     RKLFGTPLPELLSYFSLNIELMQESLEAGQGFTDNEVTLVIDGRSHILSVTAQRMPDG
                     MILLEMAPMDNQRRLSQEQLQHAQQVAARDLVRGLAHEIKNPLGGLRGAAQLLSKALP
                     DPSLLEYTKVIIEQADRLRNLVDRLLGPQLPGTRVTESIHKVAERVVTLVSMELPDNV
                     RLIRDYDPSLPELAHDPDQIEQVLLNIVRNALQALGPEGGEIILRTRTAFQLTLHGER
                     YRLAARIDVEDNGPGIPPHLQDTLFYPMVSGREGGTGLGLSIARNLIDQHSGKIEFTS
                     WPGHTEFSVYLPIRK"
     gene            14071..15480
                     /gene="glnG"
                     /locus_tag="ACHOSC_RS08770"
                     /old_locus_tag="pgaptmp_001754"
     CDS             14071..15480
                     /gene="glnG"
                     /locus_tag="ACHOSC_RS08770"
                     /old_locus_tag="pgaptmp_001754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012133942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrogen regulation protein NR(I)"
                     /protein_id="HGU4547712.1"
                     /translation="MQRGIVWVVDDDSSIRWVLERALAGAGLTCTTFENGAEVLEALA
                     SKTPDVLLSDIRMPGMDGLALLKQIKQRHPMLPVIIMTAHSDLDAAVSAYQQGAFDYL
                     PKPFDIDEAVALVERAISHYQEQQQPRNVQLNGPTTDIIGEAPAMQDVFRIIGRLSRS
                     SISVLINGESGTGKELVAHALHRHSPRAKAPFIALNMAAIPKDLIESELFGHEKGAFT
                     GANTIRQGRFEQADGGTLFLDEIGDMPLDVQTRLLRVLADGQFYRVGGYAPVKVDVRI
                     IAATHQNLEQRVQEGKFREDLFHRLNVIRVHLPPLRERREDIPRLARHFLQVAARELG
                     VEAKLLHPETEAALTRLAWPGNVRQLENTCRWLTVMAAGQEVLIQDLPGELFESTVAE
                     STSQMQPDSWATLLAQWADRALRSGHQNLLSEAQPELERTLLTTALRHTQGHKQEAAR
                     LLGWGRNTLTRKLKELGME"
     gene            15592..15702
                     /locus_tag="ACHOSC_RS08775"
                     /old_locus_tag="pgaptmp_001755"
     CDS             15592..15702
                     /locus_tag="ACHOSC_RS08775"
                     /old_locus_tag="pgaptmp_001755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YshB family small membrane protein"
                     /protein_id="HGU4547713.1"
                     /translation="MLESIINLVSSGAVDSHTPQTAVAAVLCAAMIGLFS"
     gene            complement(15892..17265)
                     /gene="hemN"
                     /locus_tag="ACHOSC_RS08780"
                     /old_locus_tag="pgaptmp_001756"
     CDS             complement(15892..17265)
                     /gene="hemN"
                     /locus_tag="ACHOSC_RS08780"
                     /old_locus_tag="pgaptmp_001756"
                     /EC_number="1.3.98.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312816.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004109 - coproporphyrinogen oxidase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oxygen-independent coproporphyrinogen III
                     oxidase"
                     /protein_id="HGU4547714.1"
                     /translation="MSVQQIDWDLALIQKYNYSGPRYTSYPTALEFSEDFGEQAFLQA
                     VARYPERPLSLYVHIPFCHKLCYFCGCNKIVTRQQHKADQYLDALEQEIVHRAPLFAG
                     RHVSQLHWGGGTPTYLNKAQISRLMKLLRENFQFNADAEISIEVDPREIELDVLDHLR
                     AEGFNRLSMGVQDFNKEVQRLVNREQDEEFIFALLNHAREIGFTSTNIDLIYGLPKQT
                     PESFAFTLKRVAELNPDRLSVFNYAHLPTIFAAQRKIKDADLPSPQQKLDILQETIAF
                     LTQSGYQFIGMDHFARPDDELAVAQREGVLHRNFQGYTTQGDTDLLGMGVSAISMIGD
                     CYAQNQKELKQYYQQVDEQGNALWRGIALTRDDCIRRDVIKSLICNFRLDYAPIEKLW
                     DLHFADYFAEDLKLLAPLAKDGLVDVDEKGIQVTAKGRLLIRNICMCFDTYLRQKARM
                     QQFSRVI"
     gene            complement(17454..17963)
                     /gene="yihI"
                     /locus_tag="ACHOSC_RS08785"
                     /old_locus_tag="pgaptmp_001757"
     CDS             complement(17454..17963)
                     /gene="yihI"
                     /locus_tag="ACHOSC_RS08785"
                     /old_locus_tag="pgaptmp_001757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Der GTPase-activating protein YihI"
                     /protein_id="HGU4547715.1"
                     /translation="MKPSSSNSRSKGHAKARRKTREELDQEARDRKRQKKRRGHAPGS
                     RAAGGNTTSGSKGQNAPKDPRIGSKTPIPLGVTEKVTKQHKPKSEKPMLSPQAELELL
                     ETDERLDALLERLEAGETLSAEEQSWVDAKLDRIDELMQKLGLSYDDDEEEEEDEKQE
                     DMMRLLRGN"
     gene            complement(18082..18248)
                     /locus_tag="ACHOSC_RS08790"
                     /old_locus_tag="pgaptmp_001758"
                     /pseudo
     CDS             complement(18082..18248)
                     /locus_tag="ACHOSC_RS08790"
                     /old_locus_tag="pgaptmp_001758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000546704.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            18545..19177
                     /gene="yihA"
                     /locus_tag="ACHOSC_RS08795"
                     /old_locus_tag="pgaptmp_001759"
     CDS             18545..19177
                     /gene="yihA"
                     /locus_tag="ACHOSC_RS08795"
                     /old_locus_tag="pgaptmp_001759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418301.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTP-binding protein
                     YihA/YsxC"
                     /protein_id="HGU4547716.1"
                     /translation="MTNLNYQQTHFVMSAPDIRHLPSDTGIEVAFAGRSNAGKSSALN
                     TLTNQKSLARTSKTPGRTQLINLFEVADGKRLVDLPGYGYAEVPEEMKRKWQRALGEY
                     LEKRQSLQGLVVLMDIRHPLKDLDQQMIEWAVDSNIAVLVLLTKADKLASGARKAQLN
                     MVREAVLAFNGDVQVETFSSLKKQGVDKLRQKLDTWFSEMQPVEETQDGE"
     gene            complement(19559..22345)
                     /gene="polA"
                     /locus_tag="ACHOSC_RS08800"
                     /old_locus_tag="pgaptmp_001760"
     CDS             complement(19559..22345)
                     /gene="polA"
                     /locus_tag="ACHOSC_RS08800"
                     /old_locus_tag="pgaptmp_001760"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006261 - DNA-templated DNA replication
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase I"
                     /protein_id="HGU4547717.1"
                     /translation="MVQIPQNPLILVDGSSYLYRAYHAFPPLTNSAGEPTGAMYGVLN
                     MLRSLIMQYKPTHAAVVFDAKGKTFRDELFEHYKSHRPPMPNDLRAQIEPLHAMVKAM
                     GLPLLAVSGVEADDVIGTLAREAEKAGRPVLISTGDKDMAQLVTPNITLINTMTNTIL
                     GPEEVVNKYGVPPELIIDFLALMGDSSDNIPGVPGVGEKTAQALLQGLGGLDTLYAEP
                     EKIAGLSFRGAKTMAAKLEQNKEVAYLSYQLATIKTDVELELTCEQLEVQQPAAEELL
                     GLFKKYEFKRWTADVEAGKWLQAKGAKPAAKPQETSVADEAPEVTATVISYDNYVTIL
                     DEETLKAWIAKLEKAPVFAFDTETDSLDNISANLVGLSFAIEPGVAAYIPVAHDYLDA
                     PDQISRERALELLKPLLEDEKALKVGQNLKYDRGILANYGIELRGIAFDTMLESYILN
                     SVAGRHDMDSLAERWLKHKTITFEEIAGKGKNQLTFNQIALEEAGRYAAEDADVTLQL
                     HLKMWPDLQKHKGPLNVFENIEMPLVPVLSRIERNGVKIDPKVLHNHSEELTLRLAEL
                     EKKAHEIAGEEFNLSSTKQLQTILFEKQGIKPLKKTPGGAPSTSEEVLEELALDYPLP
                     KVILEYRGLAKLKSTYTDKLPLMINPKTGRVHTSYHQAVTATGRLSSTDPNLQNIPVR
                     NEEGRRIRQAFIAPEDYVIVSADYSQIELRIMAHLSRDKGLLTAFAEGKDIHRATAAE
                     VFSLPLETVTSEQRRSAKAINFGLIYGMSAFGLARQLNIPRKEAQKYMDLYFERYPGV
                     LEYMERTRAQAKEQGYVETLDGRRLYLPDIKSSNGARRAAAERAAINAPMQGTAADII
                     KRAMIAVDAWLQAEQPRVRMIMQVHDELVFEVHKDDVDAVAKQIHQLMENCTRLDVPL
                     LVEVGSGENWDQAH"
BASE COUNT         5416 a         5744 c         5550 g         5789 t
ORIGIN      
        1 aaggcaacaa tctcgttctc aactctatgg agattagtta tggatacgcc acgtccacag
       61 ttattagatt ttcaatttca tcagaataac gacagtttta ccctacattt tcaacaacgt
      121 cttattttaa cccatagcaa agataatcct tgtttatgga ttggctcagg tatagcggat
      181 atcgatatgt tccgcggtaa tttcagcatt aaagataaac tacaggagaa aattgcgctt
      241 accgacgcca tcgtcagcca gtcaccggat ggttggttaa ttcatttcag ccgtggttct
      301 gacattagcg ccacgctgaa tatctctgcc gacgatcagg gccgtttatt gctggaacta
      361 caaaacgaca accttaacca caaccgtatc tggctgcgcc ttgccgctca accagaggac
      421 catatctacg gctgcggcga acagttttcc tacttcgatc tgcgtggcaa accgttcccg
      481 ctatggacca gtgaacaagg cgttggtcgc aacaaacaaa cctatgtcac ctggcaggcc
      541 gactgcaaag aaaatgcggg cggcgactat tactggactt tcttcccaca gcctacgttt
      601 gtcagcacgc agaagtatta ctgccatgtt gataacagtt gctatatgaa cttcgacttt
      661 agtgccccgg aataccatga actggcgctg tgggaagaca aagcaacgct gcgttttgaa
      721 tgtgctgaca catacatttc cctgctggaa aaattaaccg ccctgctggg acgccagcca
      781 gaactgcccg actggattta tgacggagta acgctcggca ttcagggcgg gacggaagtg
      841 tgccagaaga aactggacac catgcgtaac gcgggcgtga aggtcaacgg catctgggcg
      901 caggactggt ccggtattcg tatgacctct tttggcaaac gcgtgatgtg gaactggaag
      961 tggaacagcg aaaactaccc gcaactggat tcacgcatta agcagtggaa tcaggagggc
     1021 gtgcagttcc tggcctatat caacccgtat gttgccagcg ataatgatct ctgcgaagaa
     1081 gcggcacaac acggctatct ggcaaaagat gcctctggcg gtgactatct ggtggagttt
     1141 ggcgagtttt acggcggcgt tgtcgatctc actaatccag aagcctacgc ctggttcaag
     1201 gaagtgatca aaaagaacat gattgaactc ggctgcggcg gctggatggc tgacttcggc
     1261 gagtatctgc ccaccgacac ttacctgcat aacggcatca gcgccgaaat tatgcataac
     1321 gcctggcccg cgctgtgggc gaaatgtaac tacgaagccc ttgaagaaac gggcaagctc
     1381 ggcgagatcc ttttctttat gcgcgccggt tctaccggta gccagaaata ctccaccatg
     1441 atgtgggcgg gcgaccagaa cgtcgactgg agtctcgacg atggcctggc gtcggttgtc
     1501 ccggcggcgc tgtcgctggc aatgaccgga catggcctgc accacagcga cattggcggt
     1561 tacaccaccc tgtttgagat gaagcgcagc aaagagctgc tgctgcgctg gtgcgatttc
     1621 agcgccttca cgccgatgat gcgcacccac gaaggtaacc gtcctggcga caactggcag
     1681 tttgacggcg acgcagaaac tatcgcccat tttgcccgta tgacctccgt cttcaccacc
     1741 ctgaaacctt acctgaaaga ggctgtcgcg ctgaatgcga agtccggcct gccggttatg
     1801 cgcccgctgt tcctgcatta cgaagacgat gcgcacactt acaccctgaa atatcagtac
     1861 ctgttaggtc gcgacattct ggtcgctccg gtgcatgaag aaggccgtag cgactggacg
     1921 ctctatctgc cggaggataa ctgggtccac gcctggacgg gtgaagcgtt ccggggcggg
     1981 gaagttaccg ttaatgcgcc catcggcaag ccgccggtct tttatcgcgc cgatagcgaa
     2041 tgggcggcac tgttcgcgtc gttaaaaagc atctaatcac atccgcccgt ggggaaactc
     2101 atgggcaaag ggagaacatc gatgagtcac atcacaacgg aagatccagc aactttacgc
     2161 ctgcccttta aagagaaact ctcttacggt attggcgacc tggcctctaa catcctgctg
     2221 gatatcggta cgctttatct tttgaagttt tataccgacg ttctggggct gccaggcacc
     2281 tatggcggca ttatcttttt gatttcaaaa ttctttactg cgtttaccga tatgggtacc
     2341 ggcattatgt tggattcccg acgcaagatc ggtccaaaag gtaagttccg tccttttatt
     2401 ctgtatgcgt cattcccggt caccttactg gcgatcgcca actttgtcgg cacaccgttt
     2461 gatgtcaccg gtaaaacggt gatggccact attctgttta tgctttacgg actgtttttc
     2521 agcatgatga actgctccta cggcgcaatg gttcccgcta tcaccaaaaa ccccaacgaa
     2581 cgcgcctcac tggcggcatg gcgtcagggc ggcgctacgc tgggcctgct gctgtgcacg
     2641 gtgggattcg tgccagtgat gaatcttatc gagggtaatc agcaacttgg ctatatcttc
     2701 gccgccacgc tgttttcact gttcggcctg ctgtttatgt ggatctgcta ctcaggcgtg
     2761 aaagagcgtt atgtcgaaac ccaaccaacc aatccggcgc aaaagcctgg cctgttgcaa
     2821 tctttccgcg caattgccgg taaccgccca ctgttcattc tgtgtattgc caacctctgc
     2881 accttagggg cgtttaacgt caagctcgcc attcaggtct attacaccca gtacgtactt
     2941 aacgatccca tcctgttgtc gtatatggga tttttcagca tgggctgtat tttcatcggc
     3001 gtgttcctga tgcctggcgc agtcagacgt tttggtaaga agaaggtcta tatcggcggc
     3061 ctgctgattt gggtgctggg cgatctgctc aactatttct tcggcggcgg ctcggtcagc
     3121 tttgtggcgt tctcctgcct ggcgttcttc ggctcagcgt ttgttaacag cctgaactgg
     3181 gcgctggttt ccgacaccgt cgagtacggc gagtggcgca ccggcgtgcg ttcggaaggc
     3241 acggtctaca ccggcttcac cttctttcgc aaagtgtctc aggcgctggc tggtttcttc
     3301 cccggctgga tgctgacgca aattggctat gtgccgaacg tcgcacaggc tgaccacacc
     3361 attgaagggt tacgccagtt gatcttcatc tacccaagcg cactggcggt agtcaccatt
     3421 gtggcgatgg gttgcttcta cagcctgaac gagaagatgt atgtccgcat tgtggaagag
     3481 atagaagccc gtaaacgcac ggcgtaataa taataaacga cgccctgcgg ggcgttataa
     3541 ggagtgatta tgtctgacca taatccactg acattaaaac tgaatctgcg ggaaaaaatc
     3601 gcctatggta tgggcgacgt cggttcgaat ttaatgctct gcatcggtac tctgtatctc
     3661 ctcaaatttt ataccgatga attagggatg cctgcttact atggcgggat tatctttctg
     3721 gtggcgaagt tttttaccgc gtttaccgat atgctcaccg gatttttact cgactcgcgg
     3781 aaaaatattg ggcctaaagg caaatttcgt ccctttattt tatatgcagc agttccggcg
     3841 gcgttaattg caacgctcca gtttatcgcc accacctttt gtttgccggt taaaacgacg
     3901 attgccaccg cgctgtttat gatgtttgga ctttcataca gtctgatgaa ctgctcgtat
     3961 ggtgcgatga tcccggcaat taccaaaaac ccgaatgagc gcgcgcagct cgcggcttac
     4021 cgccagggtg gtgcaaccat agggctattg atttgtaccg tggcgtttat tccgttgcag
     4081 tcgctttttt ctgactcaac cgtcggttat gcctgtgcgg cacttatgtt ctccattggc
     4141 ggctttattt ttatgatgct gtgctacaga ggcgtcaaag agcattatgt ggacacagcg
     4201 ccaaccggac ataaagccag tattctcaaa tctttttgcg cgatatttcg taatccacca
     4261 ttgctggttt tatgcattgc taacctgtgt actctggcgg catttaatat caaactggcg
     4321 attcaggtct attacaccca gtatgtgctg aatgatatta atttattgtc gtggatggga
     4381 ttcttcagta tgggatgcat cctcgtcggc gtattactgg tgccagtaac tgtaaaatgt
     4441 tttggtaaaa aacaggttta tttagctggc atggtgctat gggcggtggg tgatatactg
     4501 aattatttct ggggaagtaa ctctttcact ttcgtcatgt tctcttgtgt cgcctttttt
     4561 ggcacggcct ttgtaaacag cctgaactgg gcactggtac cagataccgt agattacggt
     4621 gaatggaaaa ccggtattcg tgccgaaggt tctgtttata ccggttatac cttctttcgt
     4681 aaaatttctg ccgcacttgc tggcttcttg ccaggcatta tgctgacgca aattggttat
     4741 gttcccaaca tcgcgcaaag cgatgcgaca ttacagggtt tgcgtcagct catttttatc
     4801 tggccttgtg cactggcaat tattgctgca ttgacgatgg gattctttta cacactcaat
     4861 gaaaaacggt ttgcattgat tattgaggaa atcaaccaac gcaaaaataa agagatggcg
     4921 acagaagaaa aaacggcttc cgtaacgtta taaatgtcaa tagccgctat ttccatcctg
     4981 gtgggtggcg gcctccctac gtttaaaaaa tggacttatt atgaaaaaga ttaatgcaat
     5041 aattttatta tcttctctga cctctgcctc ggtatttgct ggcgcatacg tcgaaaacag
     5101 ggaggcatat aatcttgcct ccgatcaggg cgaggttatg ctacgtgtgg gttataactt
     5161 cgatatgggc gcgggtatta tgttaaccaa tacctacacc tttcagcgag aagatgaact
     5221 aaaacatggc tataatgaaa ttgaaggttg gtatccttta ttcaaaccaa ccgataaatt
     5281 aaccatccag cccggtggct taattaatga taagagtatc ggttcgggtg gtgcagtgta
     5341 tctggacgtc aactataaat ttgtaccatg gtttaatctg acagtacgaa atcgctataa
     5401 ccataataac tatagttcaa cagatttgag cggggaactg gataataatg acacctatga
     5461 aattggcacc tactggaatt ttaaaatcac cgataaattt tcctatacat ttgagccaca
     5521 ttatttcata cgcgtaaatg actttaatag cagcaatgga aaagatcatc actgggaaat
     5581 cactaatacc ttccgttatc gcattaatga acactggctg ccttatttcg aattacgttg
     5641 gttagatcgt aatgtcgaac cgtaccaccg cgagcaaaac cagattcgta tcgggacgaa
     5701 gtatttcttc tgatgtgaaa ctgccggatg cggcgtaaac gccttatccg gcctacggtt
     5761 gatacggttt tcgttggccg gattaagcac atccggccat taatcaagct tcagccgcca
     5821 gcatctcttt cttacgttgg ctaattcgct taaccagcat tacggaaacc accgcaccac
     5881 agaatgcgaa gcaggccata atgccaaaca cgattttgta gccaataatc cccggattta
     5941 aatccagaat gtagccatac agactgaagc agaacatcgc cggagcgtaa ccaataaagc
     6001 tacccaacgc catcgccgcg cctgttttat tttcagcaat ttttgcttcg ccgataggtg
     6061 caaaaaatac ggctcgctgt gtaaagacta tcgcgccaaa gcccagcgta catgccatcc
     6121 ctaaatagac cggcatactt tcgtgcggca gcataatcaa cagtacgagc gcagcggtac
     6181 tgatgataaa ggtgtagcat agatatttac tcggcgattt caggatctta tctgaaatca
     6241 taccaccaat cggtccacca atcattttca gacagtattg gttaatgatg ccgtaagccc
     6301 ccaccagcgc aacgggcaat agatagatgt ttttcaggaa tggaatgaag aatgtcaggc
     6361 cacagtaaac cgcgtaaacg aagaagacgt taaaagcgat aagccagatg gttttgtctc
     6421 tcagcaccga ggtcatactg gtgttttgcg atgctccgtc ttcttttttc acctcaacgg
     6481 acggtgcctc ttctttgtca ttcaggacaa agaaaataat aatgcctacg gcaatcacaa
     6541 taagggaata gaaccagatg cctgctttaa aacctaataa gccactgcca aaccaggtaa
     6601 atactgccaa cgcagaaaat gccaccacgg tatcgacaat gccacgccct gtttcgaaga
     6661 agccaaacaa ccgaccttgt tgttcgctat tgcccaatcg acttaccgac ttgagcaaga
     6721 ccggccagtt catcatgtcg caagtaacgc caaatagcgc ccagacaaag aggatgcccc
     6781 aataccccgg cattgtcgtc aaataaacac ccagtaatcc tgtcgcaatg agtgagaatg
     6841 acatggtgta tctgcgcggt agtttatcgg caaaataaat agacagaaaa aagcccactg
     6901 tggtgacaaa tgagtttacc gacatagcat taccaatttg accattggtc aaatggaaat
     6961 attcctgcat cgggatataa aacgcatctt tcagcgacgg taatttataa attgtaccgc
     7021 cacacaatgt taatagacta aataacgccc atttcttttt cgtgagcata acatccccta
     7081 agcgattttt aaaatcactg ttttaaaggc aataagatac cgaccattca tatggaatgg
     7141 ttttattaac tgcgtatgag ttaaaactta ttagtggttc agaagatggt ttgcctcttg
     7201 tttgatttgt tgtagtacgt tacgcacatg atttatctga ttaattgcat cttctttttc
     7261 tttacgtaac cgcgcatcca gtaaatgatt ttctggcaat ggtgtttcac tcatctggcg
     7321 ataagggatc cacggattat catcttcgtc ttcaaagcgg aacgccggcg catagtaatc
     7381 aacctcttct tccaggccat atgccgtcac ctgcggctca tcatcaccca ggcatataag
     7441 gtgcgttaat aacgctttga acggcatatc accctgacct gaaatacagg ctttatgacc
     7501 caggccaccc ggttctttaa cgatcaaggc atctttgata tggacctggg taatatgcgg
     7561 cgccatcgtt tttaaagcgt cgatgggatg ctcatttgca ttgatcatgt tcgcaaaatc
     7621 aaacagtaag gaaagagatt ccatctcact ttctttgacc agcgacacca gctcatggct
     7681 ttttaaatct tcatgctgtt cgatcgtaaa agtcaggccg ctgtcctgat acgtttcccg
     7741 tacataggca atgtcgttag cgatgatcga taatacgtcg cgcaaattac cttcataacg
     7801 tggataaaaa cgtacggacg atgccccagt tttcaacgca atggcgacgg cttcgtcaat
     7861 agatgcctta tctgaagcgc tggtttcaat atgaatatca aggttcagac ggcgggcttt
     7921 atccccaaag gcagagagtt ctttatcgtc catattacca agagaaaaac gctcgccatc
     7981 cagaacatgg attttgacgc cacgtagatt attttcactt gcgatatcaa gtaaatcagc
     8041 aggcaacact ctttccagcc gcatatttaa atgaaaggca taagcatgta aataaagagg
     8101 aagagtgtcg acacgttgta gaatctttct tgcgttattt attgtcacca taaaacacct
     8161 acagattaat gatgaggtct acggcctcaa atcgccaata ttcaatatct atctggacaa
     8221 tattgccttg ctggtcggcg cgatgctttt caattaagat cgctggcata cccgcagaac
     8281 cgccaagata cttgctgata tcgcccggca ttctgacggg tttgaaaatc aattttttta
     8341 ctaccgtctc tttttggtag cacttttccc agactgcaga aaatgagtgg ttttcaagtt
     8401 gttcaataaa acccggagca acttcaggat taatatatgt ttcgtgatag aaaactttat
     8461 gtccttccag cgccccccag ccggtgatgc gatagagttc agttgatggt gtcactgcaa
     8521 tcagtggcgc gagcgtcttg ggaatatcgc tggtacggtt tttctcggta aacccccagg
     8581 aaggctctcg tccttgctca attgcggccc gctgaaagct cgccgacaac tccggactgt
     8641 aattaaatcg cggctgggtc acgaaccacc ccttacgatc cttacggaag attttggatt
     8701 ctgcctcgag gttcaacaac gcctggcgca gcgtcatacg tttaatgccc agcagttctc
     8761 ccagctctct ttctgaaggg agttttccac ccggcgtggt gatgccatct ttcaaccacc
     8821 gatccagttt ctcttttgca ttttctacgg tggattgatt ctccgccatt gtttaatcct
     8881 tttggtttaa accagatgaa gcatcttacg cttacgccaa aaaagaaagc aattaacggc
     8941 gtatgacttt gcagataatt gtgacaaagc tctctattga cgtaatccca gacttataag
     9001 ggagatactc gcattggtgt gcgctcacga caggtaaaaa aaaacctgcc agcgatggca
     9061 ggttttttat aactaaaaaa cgaaattaat cgtctttcgg tgcgcggttg gcgcggcgac
     9121 gatcgttttc cgtcaggtga cgtttacgaa tacggataga ggtcggagtc acttctacca
     9181 gttcgtcatc atcgatgaac tccagagctt gttccagagt catgcggata ggcggaacca
     9241 gaacaacggc ttcgtcagta ccggaagcac gcatgttggt cagtttctta ccggtcaggc
     9301 agtttaccgt caggtcgtta gagcggctat gaataccgat aatctgacct tcgtaaactt
     9361 ctgcaccgtg accgaggaac agcttaccgc gatcctgcag accgaacagc gcgaacgcga
     9421 ccgctttacc ctgaccgtta gagatcagta cgccgttctg acgctgaccc acttcacccg
     9481 gacgtacgtc gtcgtagtgg ctgaaggtgg agtacagcag accagtaccg gaagtcatgg
     9541 tcatgaactc agaacggaag ccaatcagac cacggcttgg gatcacgtag tcgagacgta
     9601 cgcggccttt accgtctgga ttcatgtttt tcaggtcgcc tttacgttcg cccagcgcct
     9661 gcattacaga accctgatgc tgttcttcaa cgtccagagt cacgttttca tacggctctt
     9721 gtttacgacc gtcgatttca cggaagataa ctttcggacg ggataccgcc agttcgaaac
     9781 cttcacgacg catgttttcg atcagaacag acaggtgcag ttcgccacga ccagaaacgc
     9841 ggaacgcatc ggcgtcttcg gtttcttcta cgcgcagcgc aacgttgtgt accagttctt
     9901 tgttcagacg atccaggatc tgacgagacg ttacgaactt accttcttta ccgcagaacg
     9961 gcgaggtgtt aacgcagaag aacatagaaa cggtcggctc atcaacggag agtgccggca
    10021 gcgcttcaac gttttgcgtg tcgcaaacgg tgtcagaaat gttcagttcg ccaaggcccg
    10081 tgatcgcaac gatatcgcca gcttccgcca gatcggtttc gatacgttcc agaccgaggt
    10141 ggcccagcac tttaccgact ttcgcgttgc gggttttgcc ttcgctatcg atgatagtga
    10201 cctgctggtt cggcttcact ttaccgcgct tgatgcggcc aatgccgata acgccaacat
    10261 agctgttgta atcgagctga gaaatctgca tctggaacgg accgtcaagg tcaacgtccg
    10321 gcgcaggaac gtggtcaaca atcgcctggt acagcggggt catgtcttcc gccatatctt
    10381 cgtggtccag acccgcgata ccgttcagcg cagaagcgta aacgatcggg aagtccagct
    10441 gctcgtcggt cgcgtcgagg ttaacgaaca gatcgaatac ctgatccaca acccaatcag
    10501 gacgcgcgcc agggcggtca actttgttga taacaacaat cggcttcagg ccgtaagcaa
    10561 acgctttttt ggttacgaag cgcgtttgcg gcatcgggcc gtcaaatgcg tcaaccacca
    10621 gcagcactga gtctaccatg gacattacac gttcaacttc accaccgaag tcggcgtgcc
    10681 ccggggtatc aacgatgttg atacggtaat cattccattt gatagcggtg tttttcgcga
    10741 ggatggtaat cccccgctct ttctccaaat cgttggagtc catcacgcgc tcttgggttt
    10801 cggcacgaga gtcgaacgta ccggattgtt ggagcagctt gtctaccagg gtggttttac
    10861 catggtctac gtgcgcgatg atggcgatat tacgcaattt ttcgatcaca actttgcctc
    10921 aggcattaga aatagcgcgt tattgtacac ggattaatcg cactacaaaa caggatcaca
    10981 aacatcctcc gcaaacaagt attgcagagt ccctttgtga tcgctttcac ggagcataaa
    11041 aagggttatc caaaggtcat tgcaccaaca tggtgcttaa tgtttccatt gaagcactat
    11101 attggtgcaa cattcacatc gtggtgcagc ccttttgcac gatggtgcgc atgataacgc
    11161 cttttagggg caatttaaaa gttggcacag atttcgcttt atctttttta cggcgacacg
    11221 gccaaaataa ttgcagattt cgttaccacg acgaccatga ccaatccagg agagttaaag
    11281 tatgtccgct gaacacgtac tgacgatgct gaacgagcac gaagtgaagt ttgttgattt
    11341 gcgcttcacc gatactaaag gtaaagaaca gcacgtcact atccctgctc atcaggtgaa
    11401 tgctgaattc ttcgaagaag gcaaaatgtt tgacggctcc tcgattggcg gctggaaagg
    11461 cattaacgag tccgacatgg tgctgatgcc agacgcatcc accgcagtga ttgacccgtt
    11521 cttcgccgac tccaccctga ttatccgttg cgacatcctt gaacctggca ccctgcaagg
    11581 ctatgaccgt gacccgcgct ccattgcgaa gcgcgccgaa gattacctgc gttccactgg
    11641 cattgccgac accgtactgt tcgggccaga acctgaattc ttcctgttcg atgacatccg
    11701 tttcggatca tctatctccg gttcccacgt tgctatcgac gatatcgaag gcgcatggaa
    11761 ctcctccacc caatacgaag gtggtaacaa aggtcaccgt ccggcagtga aaggcggtta
    11821 cttcccggtt ccaccggtag actcggctca ggatattcgt tctgaaatgt gtctggtgat
    11881 ggaacagatg ggtctggtgg ttgaagccca tcaccacgaa gtagcgactg ctggtcagaa
    11941 cgaagtggct acccgcttca ataccatgac caaaaaagct gacgaaattc agatctacaa
    12001 atatgttgtg cacaacgtag cgcaccgctt cggtaaaacc gcgaccttta tgccaaaacc
    12061 gatgttcggt gataacggct ccggtatgca ctgccacatg tctctgtcta aaaacggcgt
    12121 taacctgttc gcaggcgaca aatacgcagg tctgtctgag caggcgctgt actacattgg
    12181 cggcgtaatc aaacacgcta aagcgattaa cgccctggca aacccgacca ccaactctta
    12241 taagcgtctg gtcccgggct atgaagcacc ggtaatgctg gcttactctg cgcgtaaccg
    12301 ttctgcgtct atccgtattc cggtggtttc ttctccgaaa gcacgtcgta tcgaagtacg
    12361 tttcccggat ccggcagcta acccgtacct gtgctttgct gccctgctga tggccggtct
    12421 tgatggtatc aagaacaaga tccatccggg cgaagccatg gacaaaaacc tgtatgacct
    12481 gccgccagaa gaagcgaaag agatcccaca ggttgcaggc tctctggaag aagcactgaa
    12541 cgaactggat ctggaccgcg agttcctgaa agccggtggc gtgttcactg acgaagcaat
    12601 tgatgcgtac atcgctctgc gtcgcgaaga agatgaccgc gtgcgtatga ctccgcatcc
    12661 ggtagagttt gagctgtact acagcgtcta atagttgaag ttgtactacc cggcgcaaca
    12721 acgccggagg ttcaaaccag gcccatctgg cgtaattgtt gcagccagtt tgaacacgga
    12781 cagcgcgcag aacccggagc gtacactggt acgtgaggag ttcgagcact gcccaggttc
    12841 aaaatggcaa ataaaatagc ctgatgggac tggtttttag ttgccgtgga aatttcagcc
    12901 catctctgca tgggcttttt tctccgtcaa ttctctgatg cttcgcgctt tttatccgta
    12961 aaaagctata atgcactaaa atggtgcaac ctgttcagga gactgcttta tggcaacagg
    13021 cacgcagccc gatgctgggc agatcctcaa ctcgctgatt aacagtattt tgttaatcga
    13081 tgacaacctg gcgatccatt acgccaaccc tgccgcgcaa caactgctcg cccaaagctc
    13141 ccgcaaattg tttggtacac cgttaccgga actgttgagc tacttctcat taaatatcga
    13201 gctgatgcaa gaaagtctgg aggcggggca aggttttacc gataacgaag tgacgctggt
    13261 catcgacggg cgctcgcata tcctttctgt gacggcccag cgtatgccgg acggcatgat
    13321 cctgctggag atggctccga tggataacca gcgccgctta agtcaggaac agctacagca
    13381 cgcccagcag gttgctgccc gtgatttagt gcgcggcctg gcacatgaga ttaaaaatcc
    13441 gcttggcggt ttacgtggcg cggcgcagct gctcagcaaa gcgttacctg acccatcact
    13501 actcgaatat accaaagtga ttatcgaaca ggcggaccgg ctgcgaaatc tggtcgaccg
    13561 tctgttgggg ccgcagctgc ccggtacgcg cgttaccgaa agtattcaca aagtggctga
    13621 acgcgtggta acgctggtgt cgatggaact gccggacaac gtgcggttga ttcgtgatta
    13681 cgatcccagc ctgccggaac tggcgcacga cccggatcaa attgaacagg tcttgctgaa
    13741 tattgtgcgc aatgcgctac aggcgctggg gccggaaggc ggtgaaatca ttctgcgtac
    13801 ccgcaccgcg tttcaactga ccttacacgg cgagcgctac cggctggcgg cgcggattga
    13861 tgtggaagat aacgggccgg gcattccgcc tcatttgcag gatacgctgt tttacccgat
    13921 ggtcagcggc cgcgaaggtg gcaccgggct tggcttatcc atcgctcgta atttgattga
    13981 tcagcattca ggcaaaattg aatttaccag ttggccaggg cataccgagt tctcggttta
    14041 cctgcctatc aggaaataaa ggtgacgttt atgcaacgag ggatagtctg ggtagtcgat
    14101 gacgatagtt ccatccgttg ggtgcttgaa cgtgcgctcg ctggggcagg tttaacctgt
    14161 acgacgtttg agaacggcgc agaagtgctg gaggcgctgg cgagcaaaac gccggatgtg
    14221 ctgctttcag atatccgtat gccgggaatg gacgggctgg cgctgctcaa gcagattaaa
    14281 cagcgccatc caatgcttcc ggtcatcatt atgaccgcac attccgatct ggatgctgcc
    14341 gtcagcgcct atcaacaagg ggcgtttgat tatctgccca aaccgtttga tatcgacgaa
    14401 gcagtggcgc tggttgagcg cgctatcagt cattaccagg aacagcagca gccgcgtaat
    14461 gttcagctta acggcccaac gaccgatatc atcggcgaag cgccagccat gcaggacgtg
    14521 ttccgtatta tcggtcggct ttcgcgttct tctattagcg tgctgattaa cggcgaatcc
    14581 ggcaccggta aagaactggt cgctcatgcc ctgcatcgcc acagtccgcg cgccaaagcg
    14641 ccgtttatcg cgctgaatat ggcagctatc ccaaaagatt tgatcgaatc agaactgttt
    14701 ggccacgaga aaggcgcgtt tactggcgcg aataccattc gtcaggggcg ttttgaacag
    14761 gccgatggcg gtacattatt cctcgacgaa attggtgata tgccgctgga tgtgcagacg
    14821 cgtttgctgc gcgtgctggc agacggtcag ttttaccgcg ttggcggcta tgcgccggtg
    14881 aaagtggatg tgcggattat cgctgccact caccagaatc tcgaacagcg agtgcaggaa
    14941 ggtaagttcc gtgaggatct gttccaccgc ctgaacgtta tccgcgttca tctgccgccg
    15001 ctgcgcgaac gtcgggaaga tattccccgt ctggcgcgcc attttttaca ggttgccgcg
    15061 cgcgaactgg gcgtagaagc gaagttactg catccggaaa ccgaagctgc tctgacgcgt
    15121 ctggcgtggc caggcaacgt gcgccagctg gaaaacacct gccgctggct aacggtgatg
    15181 gccgccgggc aggaagtgtt gattcaggat ttgcccggcg aactgtttga atcaacggtt
    15241 gcggagagta cttcgcaaat gcaaccggac agctgggcga cgcttcttgc gcagtgggca
    15301 gacagagcgc tgcgttccgg tcatcaaaat ctgctttccg aagcgcagcc agagctggag
    15361 cggacgttac tgacgaccgc gttgcgacat acgcaggggc ataaacagga agcggcgcgg
    15421 ctactcggct ggggccgcaa caccctgacg cgtaagttaa aagagctggg gatggagtga
    15481 ttcacagctt gtgtgtaaag attgattatt gagcgcaaat tgctggtatt ttacgcttta
    15541 ctgttccgat aagttcagta tgatcttgcc cggaaaacgg ggagagtcat tatgctggaa
    15601 tcaataatta atctggtatc gagtggcgca gttgacagcc acacaccgca aactgccgtt
    15661 gctgccgtgc tgtgtgccgc aatgattggg ctgtttagtt gagtttcaga ttgctgatga
    15721 agtgctcttt gttcatgccg gatgcggcgt gaacgcctta tccggcctac aaaagcaata
    15781 ttttcaacag gttttgtagg cctgataagc gtagcgcatc aggcaatctt gttctcagca
    15841 cattgatggc gctttcgttt tctactttgt aaacgaagcg ccattcacta tttaaatcac
    15901 ccgtgagaac tgctgcatcc gcgctttctg gcgcagatag gtatcaaagc acatgcaaat
    15961 gttgcggatc agcaagcgac ctttcgccgt cacctgtatt cccttctcat ccacatccac
    16021 cagcccatct tttgctaacg gggcgagcag cttgagatct tccgcaaagt aatcagcgaa
    16081 gtgcaaatcc cacagtttct caataggggc gtaatccaga cggaagttgc agatgagcga
    16141 cttaatcaca tcgcggcgaa tacagtcatc acgcgttagc gcaataccac gccacagcgc
    16201 attgccttgt tcatccactt gctgatagta ctgcttcaac tctttctggt tctgcgcgta
    16261 gcagtcgcca atcatgctga tggcggaaac gcccatcccc agcagatcgg tatcgccctg
    16321 agtggtgtag ccctggaagt tacgatgcag cacgccttca cgctgggcca ccgccagctc
    16381 gtcatccgga cgggcaaagt gatccatacc gataaactga tagcccgatt gcgtcaggaa
    16441 ggcgatggtt tcctgcagga tatcgagttt ttgctgcgga ctcggcaggt cagcatcttt
    16501 gattttgcgc tgagcagcaa aaatggtcgg cagatgcgcg tagttaaaga cactcagacg
    16561 atcggggttc agctccgcca cacgtttcag ggtaaaggcg aaactctccg gcgtctgttt
    16621 cggcaggccg taaatcaggt cgatgttggt ggaggtaaaa ccaatctcac gcgcatggtt
    16681 aagcagtgca aagatgaact cttcatcctg ctcgcggtta accagacgtt gcacttcttt
    16741 gttgaagtcc tgcacgccca tgctcaggcg attaaagcct tcggcgcgta aatgatcgag
    16801 tacatccagt tcgatttccc gcggatcgac ttcgatcgaa atctccgcat cggcattgaa
    16861 ctggaagttt tcgcgcagca gcttcatcag gcggctgatt tgcgctttat tcagatacgt
    16921 cggcgttccg ccgccccagt gcaattggct gacgtgacgc ccggcgaaca gcggtgcacg
    16981 atggacgatt tcttgctcca gcgcgtccag atactgatcg gccttgtgct gctggcgagt
    17041 aacaatctta ttgcaaccgc agaagtaaca aagcttatgg cagaacggga tatgtacgta
    17101 gagagataat ggacgctcag gatagcgcgc cacggcttgt aaaaacgcct gttcgccgaa
    17161 gtcttctgaa aactccagcg cggtcgggta cgaggtgtat cgtggcccgg aatagttata
    17221 tttctggatc agggccagat cccagtcgat ttgctgtaca gacatgctca ctccttccga
    17281 tggcgtctct ggcggctacg gcgtaccggc ggatgcccgt ttcgggtcat cagccggttg
    17341 cgcagccact tttgtcgccg cgacaaccgc tgtagtttaa cgaataacca caccagataa
    17401 catataaccg gaagggttat cagcagaacg ggaaggccca cggcggcaat ccgttagttg
    17461 ccccgcagca gacgcatcat gtcttcttgc ttctcgtctt cttcctcttc ttcgtcatcg
    17521 tcataagaga ggccgagttt ctgcatcaac tcatcaatac gatccagttt agcatccacc
    17581 caggattgct cttcggcgct cagggtttcg cctgcttcca gacgttccag cagcgcatcc
    17641 agacgctcat ccgtttccag taattccaac tccgcctgcg gtgaaagcat aggtttctca
    17701 ctcttcggtt tgtgctgttt ggtgactttt tcagtcacgc ccaatggaat aggggtttta
    17761 ctgccaatac gtggatcttt tggtgcgttc tggcctttgc tgcctgacgt ggtgtttccg
    17821 cccgctgcgc ggctgcccgg cgcatgacca cgacgttttt tctgacgctt gcgatcacga
    17881 gcttcctgat ccagctcctc gcgtgttttt cgacgcgctt ttgcatgacc tttgctgcgt
    17941 gagtttgaag atgatggttt cataatggtt cgtcttttag ccgttttatt cagtatagat
    18001 ttgcggcgga atctaacaga aagcaagcaa agaaaaaagg cgacagatta ctctgtcgcc
    18061 ttttttcctg actcataacc cttaacgggt cttacaatcc ttgcaggccg ccaacacacc
    18121 ctgtttttcc tttagccgga acaatccgtg ttgattccat ttccgtttaa ttacgtctcc
    18181 ggacgtttgt cttcctgaca atcctctgtc ttcgcctcct ggcgctcctg accctcatcc
    18241 tgagtcattg ttcctgttag cgtcctcgct ctatgggtgc tactttacgc ctttgcttta
    18301 aacaaacaat caaccgccat cagtatttgc acattttcag agttttatat aaaagcaatt
    18361 cactgattta tatagcttat ttgacatgca tcgcagaatt ttctctgcta tttcgcataa
    18421 agactatcgg caatgtctca caaagcgtat ggcttttact tacaacgctc acgccgaaaa
    18481 atccgccttt tattatggtt caggtataat cgccacaaag ctttgactga cggagacgac
    18541 cgctttgact aatttgaatt atcaacagac gcattttgtg atgagtgcgc ctgatattcg
    18601 ccacctacct tccgataccg gaattgaagt ggcttttgca ggccgttcca acgcaggtaa
    18661 atccagcgcg ctgaacacgc tgactaacca gaaaagcctg gctcgtacct caaaaacccc
    18721 agggcgcacc cagcttatca acctgtttga agtggctgac ggcaagcgtc tggttgactt
    18781 gcctgggtac ggttatgcgg aagtcccgga agagatgaag cgcaaatggc agcgtgcgct
    18841 cggcgaatac ctcgaaaaac gtcagagcct gcaaggtctg gtggtgctaa tggatattcg
    18901 ccatccgctg aaagatttgg atcagcagat gattgagtgg gcggtagaca gcaatatcgc
    18961 cgttctggtg ctgctgacca aagcggacaa actggcaagc ggcgcacgta aagcgcaatt
    19021 gaatatggtg cgtgaagctg tactggcgtt taacggtgat gtgcaggttg aaacgttttc
    19081 ttcgttgaag aaacaaggcg tggacaagct gcggcagaaa ctggatacct ggtttagcga
    19141 gatgcagcct gtagaagaaa cgcaggacgg cgaataattt tcttgcctta atgcttgtgc
    19201 cggatgtggc gtatccggcc cgtaaattca atagattaca attctaccgt tggcctgata
    19261 aacatagcgc atcaggcatt acggatcttt tctttcgccc aataaaaaac gccccagtca
    19321 ttactgactg gggcggctaa aatattcagc caaatccgat tacgtgaagt aaaaggtctg
    19381 aaagatagaa catcttacct ctgtacccta cgcgactaac tttactcttt tttgttcagt
    19441 tcagaaagca ctttttgtaa ttttttttca gttgttgcat aggaatttct aatagccatc
    19501 acaaaacgtg acagcttatg ttgcttactt acgaaaaaag ggcatgttca ggcgaatctt
    19561 agtgcgcctg atcccagttt tcgccactcc ccacttccac cagcaacggc acatccagac
    19621 gggtacagtt ttccatcagt tgatgaatct gcttcgcgac ggcatcaaca tcatctttat
    19681 gaacttcaaa taccagttca tcgtgtacct gcatgatcat acgtacacgc ggttgctcag
    19741 cctgtaacca cgcatcaacg gcaatcatcg cccgtttgat aatgtcggcg gcggttccct
    19801 gcattggcgc gttaatggct gcacgttcag ccgctgcacg acgagcacca ttgctggatt
    19861 tgatatccgg cagatacaga cggcgtccgt ccagcgtttc aacgtagccc tgctctttcg
    19921 cctgagcacg ggtgcgttcc atatactcca gcacgccagg gtagcgttcg aagtaaaggt
    19981 ccatgtactt ctgcgcttct ttacgtggaa tgttcaattg ccgcgccaga ccgaaagcac
    20041 tcatgccata aatcagacca aagttgatcg ctttcgcgct acggcgttgc tcgctggtga
    20101 cggtttccag tggcaaacta aacacttctg ccgccgttgc ccggtggata tcttttcctt
    20161 ccgcgaatgc ggtcagcaag cctttgtcac gcgaaagatg cgccataatg cgcagttcaa
    20221 tctgcgagta gtccgctgag acaatcacat aatcctctgg cgcaataaac gcctggcgga
    20281 tacgacgacc ttcttcgtta cgcaccggaa tgttttgcag gttaggatcg gttgacgata
    20341 aacgtcccgt tgcagttact gcctggtgat aagaggtatg cacacgcccg gttttcgggt
    20401 tgatcatcag cggcagcttg tcggtgtagg tcgatttcag cttcgccaga ccacgatact
    20461 ccagaatcac ttttggcaac ggatagtcca gcgccagttc ttccagtacc tcttccgacg
    20521 ttgacggcgc gccacccggc gttttcttca gcggtttaat accctgtttt tcaaagagaa
    20581 tggtttgcaa ctgcttggtg gaagaaaggt taaattcttc acctgcaatt tcatgcgctt
    20641 tcttttccag ctcagccaga cgcaaggtga gctcttcaga atgattgtgc agcactttcg
    20701 gatcgatctt cacaccgtta cgttcaatgc gtgaaagcac cggcaccagc ggcatttcga
    20761 tattctcgaa gacgttcaac ggccctttgt gtttttgcag atctggccac attttcagat
    20821 gcaactgcaa ggtgacatca gcatcttcgg cggcgtaacg tccggcctct tcgagggcaa
    20881 tctggttaaa ggtcagttga tttttgcctt tacccgcaat ctcttcaaaa gtgatggttt
    20941 tgtgcttcaa ccaacgttcc gcgaggctgt ccatatcgtg acgcccggca acgctattga
    21001 gaatgtagga ctccagcatg gtatcaaacg caatcccacg cagctcaatg ccgtaattcg
    21061 ccagaatacc gcgatcgtat ttcaggtttt gcccgacctt cagcgccttt tcatcttcca
    21121 gcagcggttt tagcaactcg agtgcacgct cgcgagagat ttgatcgggc gcgtcaagat
    21181 aatcatgagc aaccggaata tatgccgcta cgcctggctc gatagcaaaa gaaagcccga
    21241 ccaggttagc agagatgtta tcaaggctgt cggtttcggt atcaaatgca aataccggcg
    21301 ctttttccag cttcgcaatc cacgctttca gtgtttcttc atcaaggatg gtgacgtagt
    21361 tgtcataaga aatcaccgtt gccgtcactt ctggcgcttc gtctgcaaca ctggtttcct
    21421 gcggcttcgc ggctggtttt gcccctttgg cctgtaacca tttgcccgct tcgacatcag
    21481 cagtccagcg tttgaactca tactttttga acagccccaa caactcttcc gctgccggtt
    21541 gctgcacttc cagttgttca caggtcagct ccagttcaac gtcggtttta atcgtcgcca
    21601 gctggtatga gagataagca acttctttgt tttgctcgag cttcgctgcc attgttttcg
    21661 cgccacggaa gctcaaccca gcaatttttt ctggctcggc atacagcgta tccagcccgc
    21721 caagaccttg cagcaatgcc tgcgcggttt tttcaccgac gcccggtacg ccaggaatgt
    21781 tatcggagga gtcacccatc agcgccagga aatcgatgat cagttctggc ggcacgccgt
    21841 acttattcac cacctcttcc ggtccgagga tggtattcgt catggtattg ataagcgtaa
    21901 tatttggcgt caccagctgc gccatatctt tatcgccagt gctgatcagc accggacgcc
    21961 cggctttttc ggcttcgcgc gccagagtac cgataacgtc gtccgcttct acgccagaaa
    22021 ccgccagcag cggcagtccc atcgctttaa ccatcgcgtg caggggttcg atttgtgctc
    22081 gcagatcgtt cggcattggc gggcgatgtg atttgtaatg ttcaaacagt tcatcacgaa
    22141 aggtttttcc cttggcgtca aagaccaccg ctgcatgcgt cggtttatat tgcatgatca
    22201 gactgcgcag catgttgagg acaccataca tcgcaccggt cggctcgcct gcgctgttag
    22261 tcagcggggg aaacgcgtga tatgcgcgat aaagataaga tgaaccatct acaaggataa
    22321 gtggattttg ggggatctga accataatgt ccgtgcctgt ttatcagatt atgggtaaag
    22381 gatgccacag acaggatgaa aacatgagtt tttcaccgct tttcgtgaaa atcttttgta
    22441 gatcttctgg atcgctcgca aatcgtcatg tggataactt tgtgcataaa ttatttaca
//
LOCUS       DAZKHF020000057        22058 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2201S31241
            SAMN43267800-rid25106413.denovo.057, whole genome shotgun sequence.
ACCESSION   DAZKHF020000057 DAZKHF020000000
VERSION     DAZKHF020000057.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267800
            Sequence Read Archive: SRR30307263
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 22058)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22058)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 22058)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350581.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350581.2
            Genome Coverage       :: 151x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:21:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,652
            CDSs (total)                      :: 5,557
            Genes (coding)                    :: 5,320
            CDSs (with protein)               :: 5,320
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 237
            CDSs (without protein)            :: 237
            Pseudo Genes (ambiguous residues) :: 0 of 237
            Pseudo Genes (frameshifted)       :: 102 of 237
            Pseudo Genes (incomplete)         :: 130 of 237
            Pseudo Genes (internal stop)      :: 52 of 237
            Pseudo Genes (multiple problems)  :: 41 of 237
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22058
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267800-rid25106413.denovo.057"
                     /serotype="O 26:H11"
                     /isolate="2201S31241"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-01"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            <1..850
                     /locus_tag="ACHOSC_RS08805"
                     /old_locus_tag="pgaptmp_001761"
     CDS             <1..850
                     /locus_tag="ACHOSC_RS08805"
                     /old_locus_tag="pgaptmp_001761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417258.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="YgcG family protein"
                     /protein_id="HGU4547718.1"
                     /translation="TFVCSFVAAQPTIVPQLQQQVTDLTSSLNSQEKKELTHKLESIF
                     NNTQVQIAVLIVPTTKDETIEQYATRVFDNWRLGDAKRNDGILIIVAWSDRTVRIQVG
                     YGLEEKVTDALAGDIIRSNMIPAFKQQKFAQGLELAINALNNQLTSQHQYPTNPSESE
                     SASSSDHYYFAIFWVFAVMFFPFWFFHQGSNFCRACKSGVYISAIYLLDLFLFSDKIF
                     SIAVFSFFFTFTIFMVFTCLCVLQKRASGKSYHSDNSGSGGGSSGGGFSGGGGSSGGG
                     GASGRW"
     gene            complement(910..2208)
                     /gene="eno"
                     /locus_tag="ACHOSC_RS08810"
                     /old_locus_tag="pgaptmp_001762"
     CDS             complement(910..2208)
                     /gene="eno"
                     /locus_tag="ACHOSC_RS08810"
                     /old_locus_tag="pgaptmp_001762"
                     /EC_number="4.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000015 - phosphopyruvate hydratase
                     complex [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004634 - phosphopyruvate hydratase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopyruvate hydratase"
                     /protein_id="HGU4547719.1"
                     /translation="MSKIVKIIGREIIDSRGNPTVEAEVHLEGGFVGMAAAPSGASTG
                     SREALELRDGDKSRFLGKGVTKAVAAVNGPIAQALIGKDAKDQAGIDKIMIDLDGTEN
                     KSKFGANAILAVSLANAKAAAAAKGMPLYEHIAELNGTPGKYSMPVPMMNIINGGEHA
                     DNNVDIQEFMIQPVGAKTVKEAIRMGSEVFHHLAKVLKAKGMNTAVGDEGGYAPNLGS
                     NAEALAVIAEAVKAAGYELGKDITLAMDCAASEFYKDGKYVLAGEGNKAFTSEEFTHF
                     LEELTKQYPIVSIEDGLDESDWDGFAYQTKVLGDKIQLVGDDLFVTNTKILKEGIEKG
                     IANSILIKFNQIGSLTETLAAIKMAKDAGYTAVISHRSGETEDATIADLAVGTAAGQI
                     KTGSMSRSDRVAKYNQLIRIEEALGEKAPYNGRKEIKGQA"
     gene            complement(2296..3933)
                     /gene="pyrG"
                     /locus_tag="ACHOSC_RS08815"
                     /old_locus_tag="pgaptmp_001763"
     CDS             complement(2296..3933)
                     /gene="pyrG"
                     /locus_tag="ACHOSC_RS08815"
                     /old_locus_tag="pgaptmp_001763"
                     /EC_number="6.3.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005130912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003883 - CTP synthase activity [Evidence
                     IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine hydrolyzing CTP synthase"
                     /protein_id="HGU4547720.1"
                     /translation="MTTNYIFVTGGVVSSLGKGIAAASLAAILEARGLNVTIMKLDPY
                     INVDPGTMSPIQHGEVFVTEDGAETDLDLGHYERFIRTKMSRRNNFTTGRIYSDVLRK
                     ERRGDYLGATVQVIPHITNAIKERVLEGGEGHDVVLVEIGGTVGDIESLPFLEAIRQM
                     AVEIGREHTLFMHLTLVPYMAASGEVKTKPTQHSVKELLSIGIQPDILICRSDRAVPA
                     NERAKIALFCNVPEKAVISLKDVDSIYKIPGLLKSQGLDDYICKRFSLNCPEANLSEW
                     EQVIFEEANPVSEVTIGMVGKYIELPDAYKSVIEALKHGGLKNRVSVNIKLIDSQDVE
                     TRGVEILKGLDAILVPGGFGYRGVEGMITTARFARENNIPYLGICLGMQVALIDYARH
                     VANMENANSTEFVPDCKYPVVALITEWRDENGNVEVRSEKSDLGGTMRLGAQQCQLVD
                     DSLVRQLYNAPTIVERHRHRYEVNNMLLKQIEDAGLRVAGRSGDDQLVEIIEVPNHPW
                     FVACQFHPEFTSTPRDGHPLFAGFVKAASEFQKRQAK"
     gene            complement(4161..4952)
                     /gene="mazG"
                     /locus_tag="ACHOSC_RS08820"
                     /old_locus_tag="pgaptmp_001764"
     CDS             complement(4161..4952)
                     /gene="mazG"
                     /locus_tag="ACHOSC_RS08820"
                     /old_locus_tag="pgaptmp_001764"
                     /EC_number="3.6.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside triphosphate pyrophosphohydrolase"
                     /protein_id="HGU4547721.1"
                     /translation="MNQIDRLLTIMQRLRDPENGCPWDKEQTFATIAPYTLEETYEVL
                     DAIAREDFDDLRGELGDLLFQVVFYAQMAQEEGRFDFNDICAAISDKLERRHPHVFAD
                     SSAENSSEVLARWEQIKTEERAQKAQHSALDDIPRSLPALMRAQKIQKRCANVGFDWT
                     TLGPVVDKVYEEIDEVMYEARQAVVDQAKLEEEMGDLLFATVNLARHLGTKAEIALQK
                     ANEKFERRFREVERIVAARGLEMTGVDLETMEEVWQQVKRQEIDL"
     gene            complement(5023..5358)
                     /gene="mazF"
                     /locus_tag="ACHOSC_RS08825"
                     /old_locus_tag="pgaptmp_001765"
     CDS             complement(5023..5358)
                     /gene="mazF"
                     /locus_tag="ACHOSC_RS08825"
                     /old_locus_tag="pgaptmp_001765"
                     /EC_number="3.1.27.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endoribonuclease MazF"
                     /protein_id="HGU4547722.1"
                     /translation="MVSRYVPDMGDLIWVDFDPTKGSEQAGHRPAVVLSPFMYNNKTG
                     MCLCVPYTTQSKGYPFEVVLSGQERDGVALADQVKSIAWRARGATKKGTVAPEELQLI
                     KAKINVLIG"
     gene            complement(5358..5606)
                     /gene="mazE"
                     /locus_tag="ACHOSC_RS08830"
                     /old_locus_tag="pgaptmp_001766"
     CDS             complement(5358..5606)
                     /gene="mazE"
                     /locus_tag="ACHOSC_RS08830"
                     /old_locus_tag="pgaptmp_001766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system antitoxin MazE"
                     /protein_id="HGU4547723.1"
                     /translation="MIHSSVKRWGNSPAVRIPATLMQALNLNIDDEVKIDLVDGKLII
                     EPVRKEPVFTLAELVNDITPENLHENIDWGEPKDKEVW"
     gene            complement(5684..7918)
                     /gene="relA"
                     /locus_tag="ACHOSC_RS08835"
                     /old_locus_tag="pgaptmp_001767"
     CDS             complement(5684..7918)
                     /gene="relA"
                     /locus_tag="ACHOSC_RS08835"
                     /old_locus_tag="pgaptmp_001767"
                     /EC_number="2.7.6.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015969 - guanosine tetraphosphate
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP diphosphokinase"
                     /protein_id="HGU4547724.1"
                     /translation="MVAVRSAHINKAGEFDPEKWIASLGITSQKSCECLAETWAYCLQ
                     QTQGHPDASLLLWRGVEMVEILSTLSMDIDTLRAALLFPLADANVVSEDVLRESVGKS
                     VVNLIHGVRDMAAIRQLKATHTDSVSSEQVDNVRRMLLAMVDDFRCVVIKLAERIAHL
                     REVKDAPEDERVLAAKECTNIYAPLANRLGIGQLKWELEDYCFRYLHPTEYKRIAKLL
                     HERRLDREHYIEEFVGHLRAEMKAEGVKAEVYGRPKHIYSIWRKMQKKNLAFDELFDV
                     RAVRIVAERLQDCYAALGIVHTHYRHLPDEFDDYVANPKPNGYQSIHTVVLGPGGKTV
                     EIQIRTKQMHEDAELGVAAHWKYKEGAAAGGARSGHEDRIAWLRKLIAWQEEMADSGE
                     MLDEVRSQVFDDRVYVFTPKGDVVDLPAGSTPLDFAYHIHSDVGHRCIGAKIGGRIVP
                     FTYQLQMGDQIEIITQKQPNPSRDWLNPNLGYVTTSRGRSKIHAWFRKQDRDKNILAG
                     RQILDDELEHLGISLKEAEKHLLPRYNFNDVDELLAAIGGGDIRLNQMVNFLQSQFNK
                     PSAEEQDAAALKQLQQKSYTPQNRSKDNGRVVVEGVGNLMHHIARCCQPIPGDEIVGF
                     ITQGRGISVHRADCEQLAELRSHAPERIVDAVWGESYSAGYSLVVRVVANDRSGLLRD
                     ITTILANEKVNVLGVASRSDTKQQLATIDMTIEIYNLQVLGRVLGKLNQVPDVIDARR
                     LHGS"
     gene            complement(7966..9267)
                     /gene="rlmD"
                     /locus_tag="ACHOSC_RS08840"
                     /old_locus_tag="pgaptmp_001768"
     CDS             complement(7966..9267)
                     /gene="rlmD"
                     /locus_tag="ACHOSC_RS08840"
                     /old_locus_tag="pgaptmp_001768"
                     /EC_number="2.1.1.190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uracil(1939)-C(5))-methyltransferase
                     RlmD"
                     /protein_id="HGU4547725.1"
                     /translation="MAQFYSAKRRTTTRQIITVSVNDLDSFGQGVARHNGKTLFIPGL
                     LPQENAEVAVTEDKKQYARAKVVRRLSDSPERETPRCPHFGVCGGCQQQHASVDLQQR
                     SKSAALARLMKHDVSEVIADVPWGYRRRARLSLNYLPKTQQLQMGFRKAGSSDIVDVK
                     QCPILVPQLEALLPKVRACLGSLQVMRHLGHVELVQATSGTLMILRHTAPLSSADREK
                     LERFSHSEGLDLYLAPDSEILETVSGEMPWYDSNGLRLTFSPRDFIQVNAGVNQKMVA
                     RALEWLDVQPEDRVLDLFCGMGNFTLPLAKQAASVVGVEGVPALVEKGQQNARLNGLQ
                     NVTFYHENLEEDVTKQPWAKNGFDKVLLDPARAGAAGVMQQIIKLEPIRIVYVSCNPA
                     TLARDSEALLKAGYTIARLAMLDMFPHTGHLESMVLFSRVK"
     gene            9324..12080
                     /gene="barA"
                     /locus_tag="ACHOSC_RS08845"
                     /old_locus_tag="pgaptmp_001769"
     CDS             9324..12080
                     /gene="barA"
                     /locus_tag="ACHOSC_RS08845"
                     /old_locus_tag="pgaptmp_001769"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component sensor histidine kinase BarA"
                     /protein_id="HGU4547726.1"
                     /translation="MTNYSLRARMMILILAPTVLIGLLLSIFFVVHRYNDLQRQLEDA
                     GASIIEPLAVSTEYGMSLQNRESIGQLISVLHRRHSDIVRAISVYDENNRLFVTSNFH
                     LDPSSMQLGSNVPFPRQLTVTRDGDIMILRTPIISESYSPDESPSSDAKNSQNMLGYI
                     ALELDLKSVRLQQYKEIFISSVMMLFCIGIALIFGWRLMRDVTGPIRNMVNTVDRIRR
                     GQLDSRVEGFMLGELDMLKNGINSMAMSLAAYHEEMQHNIDQATSDLRETLEQMEIQN
                     VELDLAKKRAQEAARIKSEFLANMSHELRTPLNGVIGFTRLTLKTELTPTQRDHLNTI
                     ERSANNLLAIINDVLDFSKLEAGKLILESIPFPLRSTLDEVVTLLAHSSHDKGLELTL
                     NIKSDVPDNVIGDPLRLQQIITNLVGNAIKFTENGNIDILVEKRALSNTKVQIEVQIR
                     DTGIGIPERDQSRLFQAFRQADASISRRHGGTGLGLVITQKLVNEMGGDISFHSQPNR
                     GSTFWFHINLDLNPNIIIEGPSTQCLAGKRLAYVEPNSAAAQCTLDILSETPLEVVYS
                     PTFSALPPAHYDMMLLGIAVTFREPLTMQHERLAKAVSMTDFLMLALPCHAQVNAEKL
                     KQDGIGACLLKPLTPTRLLPALTEFCHHKQNTLLPVTDESKLAMTVMAVDDNPANLKL
                     IGALLEDMVQHVELCDSGHQAVERAKQMPFDLILMDIQMPDMDGIRACELIHQLPHQQ
                     QTPVIAVTAHAMAGQKEKLLGAGMSDYLAKPIEEERLHNLLLRYKPGSGISSRVVTPE
                     VNEIVVNPNATLDWQLALRQAAGKTDLARDMLQMLLDFLPEVRNKVEEQLVGENPEGL
                     VDLIHKLHGSCGYSGVPRMKNLCQLIEQQLRSGTKEEDLEPELLELLDEMDNVAREAS
                     KILG"
     gene            complement(12312..13652)
                     /gene="gudD"
                     /locus_tag="ACHOSC_RS08850"
                     /old_locus_tag="pgaptmp_001770"
     CDS             complement(12312..13652)
                     /gene="gudD"
                     /locus_tag="ACHOSC_RS08850"
                     /old_locus_tag="pgaptmp_001770"
                     /EC_number="4.2.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008872 - glucarate dehydratase activity
                     [Evidence IEA];
                     GO_process: GO:0019394 - glucarate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucarate dehydratase"
                     /protein_id="HGU4547727.1"
                     /translation="MSSQFTTPVVTEMQVIPVAGHDSMLMNLSGAHAPFFTRNIVIIK
                     DNSGHTGVGEIPGGEKIRKTLEDAIPLVVGKTLGKYKNVLTLVRNTFADRDAGGRGLQ
                     TFDLRTTIHVVTGIEAAMLDLLGQHLGVNVASLLGDGQQRSEVEMLGYLFFVGDRKAT
                     PLPYQSQPDDSCDWYRLRHEEAMTPDAVVRLAEAAYEKYGFNDFKLKGGVLAGEEEAE
                     SIVALAQRFPQARITLDPNGAWSLNEAIKIGKYLKGSLAYAEDPCGAEQGFSGREVMA
                     EFRRATGLPTATNMIATDWRQMGHTLSLQSVDIPLADPHFWTMQGSVRVAQMCHEFGL
                     TWGSHSNNHFDISLAMFTHVAAAAPGKITAIDTHWIWQEGNQRLTKEPFEIKGGLVQV
                     PEKPGLGVEIDMDQVMKAHELYQKHGLGARDDAMGMQYLIPGWTFDNKRPCMVR"
     gene            complement(13673..15013)
                     /locus_tag="ACHOSC_RS08855"
                     /old_locus_tag="pgaptmp_001771"
     CDS             complement(13673..15013)
                     /locus_tag="ACHOSC_RS08855"
                     /old_locus_tag="pgaptmp_001771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708582.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enolase C-terminal domain-like protein"
                     /protein_id="HGU4547728.1"
                     /translation="MTTQSSPVITDMKVIPVAGHDSMLLNIGGAHNAYFTRNIVVLTD
                     NAGHTGIGEAPGGEVIYQTLVDAIPMVLGQEVARLNKVVQQVHKGNQAADFDTFGKGA
                     WTFELRVNAVAALEAALLDLLGKALNVPVCELLGPGKQRETITVLGYLFYIGDRTKTD
                     LPYLENTPGNHEWYQLRHQKAMNSEAVVRLAEASQDRYGFKDFKLKGGVLPGEQEIDT
                     VRALKKRFPDARITVDPNGAWLLDEAISLCKGLNDVLTYAEDPCGAEQGFSGREVMAE
                     FRRATGLPVATNMIATNWREMGHAVMLNAVDIPLADPHFWTLSGAVRVAQLCDDWGLT
                     WGCHSNNHFDISLAMFTHVGAAAPGNPTAIDTHWIWQEGDCRLTKNPLEIKNGKIAVP
                     DAPGLGVELDWEQVQKAHEAYKRLPGGARNDAGPMQYLIPGWTFDRKRPVFGRH"
     gene            complement(15015..16367)
                     /gene="gudP"
                     /locus_tag="ACHOSC_RS08860"
                     /old_locus_tag="pgaptmp_001772"
     CDS             complement(15015..16367)
                     /gene="gudP"
                     /locus_tag="ACHOSC_RS08860"
                     /old_locus_tag="pgaptmp_001772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactarate/glucarate/glycerate transporter
                     GudP"
                     /protein_id="HGU4547729.1"
                     /translation="MSSLSQAASSVEKRTNARYWIVVMLFIVTSFNYGDRATLSIAGS
                     EMAKDIGLDPVGMGYVFSAFSWAYVIGQIPGGWLLDRFGSKRVYFWSIFIWSMFTLLQ
                     GFVDIFSGFGIIVALFTLRFLVGLAEAPSFPGNSRIVAAWFPAQERGTAVSIFNSAQY
                     FATVIFAPIMGWLTHEVGWSHVFFFMGGLGIVISFIWLKVIHEPNQHPGVNQKELEYI
                     AAGGALINMDQQNTKVKVPFSVKWGQIKQLLGSRMMIGVYIGQYCINALTYFFITWFP
                     VYLVQARGMSILKAGFVASVPAVCGFIGGVLGGIISDWLMRRTGSLNIARKTPIVMGM
                     LLSMVMVFCNYVNVEWMIIGFMALAFFGKGIGALGWAVMADTAPKEISGLSGGLFNMF
                     GNISGIVTPIAIGYIVGTTGSFNGALIYVGVHALIAVLSYLVLVGDIKRIELKPVAGQ
                     "
     gene            complement(16802..17251)
                     /locus_tag="ACHOSC_RS08865"
                     /old_locus_tag="pgaptmp_001773"
     CDS             complement(16802..17251)
                     /locus_tag="ACHOSC_RS08865"
                     /old_locus_tag="pgaptmp_001773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin"
                     /protein_id="HGU4547730.1"
                     /translation="MAEIGIFVGTMYGNSLLVAEEAEAILTAQGHKATVFEDPELSDW
                     LPYQDKYVLVVTSTTGQGDLPDSIVPLFQGIKDSLGFQPNLRYGVIALGDSSYVNFCN
                     GGKQFDALLQEQSAQRVGEILLIDASENPEPETESNPWVEQWGTLLS"
     gene            complement(17269..18051)
                     /gene="truC"
                     /locus_tag="ACHOSC_RS08870"
                     /old_locus_tag="pgaptmp_001774"
     CDS             complement(17269..18051)
                     /gene="truC"
                     /locus_tag="ACHOSC_RS08870"
                     /old_locus_tag="pgaptmp_001774"
                     /EC_number="5.4.99.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(65) synthase TruC"
                     /protein_id="HGU4547731.1"
                     /translation="MLEILYQDEWLVAVNKPSGWLVHRSWLDRDEKVVVMQTVRDQIG
                     QHVFTAHRLDRPTSGVLLMGLSSEAGRLLAQQFEQHQIQKRYHAIVRGWLMEEAVLDY
                     PLVEELDKIADKFAREDKGSQPAVTHYRGLATVEMPVATGRYPTTRYGLVELEPKTGR
                     KHQLRRHLAHLRHPIIGDSKHGDLRQNRSGAEHFGLQRLMLHASQLSLTHPFTGEPLT
                     IHAGLDDTWMQALSQFGWRGLLPENERVEFSAPSGQDGEISS"
     gene            complement(18051..18380)
                     /locus_tag="ACHOSC_RS08875"
                     /old_locus_tag="pgaptmp_001775"
     CDS             complement(18051..18380)
                     /locus_tag="ACHOSC_RS08875"
                     /old_locus_tag="pgaptmp_001775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqcC family protein"
                     /protein_id="HGU4547732.1"
                     /translation="MTTHDRVRLQLQALEALLREHQHWRNDEPQPHQFNSTQPFFMDT
                     MEPLEWLQWVLIPRMHDLLDNKQPLPGAFAVAPYYEMALATDHPQRALILAELEKLDA
                     LFADDAS"
     gene            complement(19002..19547)
                     /gene="syd"
                     /locus_tag="ACHOSC_RS08880"
                     /old_locus_tag="pgaptmp_001776"
     CDS             complement(19002..19547)
                     /gene="syd"
                     /locus_tag="ACHOSC_RS08880"
                     /old_locus_tag="pgaptmp_001776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SecY-interacting protein"
                     /protein_id="HGU4547733.1"
                     /translation="MDDLTAQALKDFTARYCDAWHEEHKSWPLSEELYGVPSPCIIST
                     TEDAVYWQPQPFTGEQNVNAVERAFDIVIQPTIHTFYTTQFAGDMHAQFGDIKLTLLQ
                     TWSEDDFRRVQENLIGHLVTQKRLKLPPTLFIATLEEELEVISVCNLSGEVCKETLGT
                     RKRTHLASNLAEFLNQLKPLL"
     gene            19615..20463
                     /gene="queF"
                     /locus_tag="ACHOSC_RS08885"
                     /old_locus_tag="pgaptmp_001777"
     CDS             19615..20463
                     /gene="queF"
                     /locus_tag="ACHOSC_RS08885"
                     /old_locus_tag="pgaptmp_001777"
                     /EC_number="1.7.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708588.1"
                     /note="Catalyzes the NADPH-dependent reduction of
                     7-cyano-7-deazaguanine (preQ0) to
                     7-aminomethyl-7-deazaguanine (preQ1) in queuosine
                     biosynthesis; Derived by automated computational analysis
                     using gene prediction method: Protein Homology.
                     GO_function: GO:0033739 - preQ1 synthase activity
                     [Evidence IEA];
                     GO_function: GO:0043867 - 7-cyano-7-deazaguanine
                     tRNA-ribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0008616 - queuosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent 7-cyano-7-deazaguanine reductase
                     QueF"
                     /protein_id="HGU4547734.1"
                     /translation="MSSYANHQALAGLTLGKSTDYRDTYDANLLQGVPRSLNRDPLGL
                     KADNLPFHGTDIWTLYELSWLNAKGLPQVAVGHVELDYTSANLIESKSFKLYLNSFNQ
                     TRFNNWDEVRQTLERDLSTCAQGKVSVALYRLDELEGQPIGHFNGTCIDDQDITIDNY
                     EFTTDYLENATSGEKVVEETLVSHLLKSNCLITHQPDWGSIQIQYRGRQIDREKLLRY
                     LVSFRHHNEFHEQCVERIFNDLLRFCQPEKLSVYARYTRRGGLDINPWRSNSDFVPST
                     TRLVRQ"
     gene            20575..21939
                     /gene="ppnN"
                     /locus_tag="ACHOSC_RS08890"
                     /old_locus_tag="pgaptmp_001778"
     CDS             20575..21939
                     /gene="ppnN"
                     /locus_tag="ACHOSC_RS08890"
                     /old_locus_tag="pgaptmp_001778"
                     /EC_number="3.2.2.10"
                     /EC_number="3.2.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide 5'-monophosphate nucleosidase PpnN"
                     /protein_id="HGU4547735.1"
                     /translation="MITHISPLGSMDMLSQLEVDMLKRTASSDLYQLFRNCSLAVLNS
                     GSLTDNSKELLSRFENFDINVLRRERGVKLELINPPEEAFVDGRIIRALQANLFAVLR
                     DILFVYGQIHNTVRFPNLNLDNSVHITNLVFSILRNARALHVGEAPNMVVCWGGHSIN
                     ENEYLYARRVGNQLGLRELNICTGCGPGAMEAPMKGAAVGHAQQRYKDSRFIGMTEPS
                     IIAAEPPNPLVNELIIMPDIEKRLEAFVRIAHGIIIFPGGVGTAEELLYLLGILMNPA
                     NKDQVLPLILTGPKESADYFRVLDEFVVHTLGENARRHYRIIIDDAAEVARQMKKSMP
                     LVKENRRDTGDAYSFNWSMRIAPDLQMPFEPSHENMANLKLYPDQPVEVLAADLRRAF
                     SGIVAGNVKEVGIRAIEEFGPYKINGDKEIMRRMDDLLQGFVAQHRMKLPGSAYIPCY
                     EICT"
BASE COUNT         5405 a         5893 c         5239 g         5521 t
ORIGIN      
        1 cactttcgtg tgttcctttg ttgcagccca accaactatt gtcccacaat tacagcaaca
       61 agttacggat cttacgagta gcttaaactc acaagaaaag aaagaactga cgcacaagtt
      121 agaatctatt ttcaataata cccaagtgca aattgctgta ttaattgttc ccacaaccaa
      181 agacgagaca attgaacaat atgctacaag agtttttgac aattggcgtt taggagatgc
      241 caaacgtaat gatgggatac tgatcattgt tgcctggtca gatcgtactg tccgcatcca
      301 ggtaggttat gggctggaag aaaaggtaac cgatgctctg gctggagata tcatccgtag
      361 caacatgata cccgcattta aacaacaaaa atttgctcag ggattagagc tagctataaa
      421 cgctttgaat aaccaactca cttcacaaca ccaatatccg actaatcctt cagaaagtga
      481 atcagcgtct tccagtgatc attattactt tgctattttt tgggtatttg cagtgatgtt
      541 cttccctttc tggttttttc atcaaggcag taatttttgt cgcgcatgta aaagtggagt
      601 ctatatttca gcaatctatc ttttagattt attcctgttc tccgataaaa ttttttccat
      661 tgctgtattt tccttctttt ttactttcac catatttatg gtctttacct gtttatgtgt
      721 acttcagaaa agagcatcag gtaaaagtta tcattcagac aacagcggtt ccggtggagg
      781 ctcaagtggc ggcggattta gcggcggtgg cggttcttct ggaggtggcg gcgcatccgg
      841 ccgctggtaa ctcaaccata aaaaatgcca gcccggaggc tggcattttt aaatcagata
      901 aagtcagtct tatgcctggc ctttgatctc tttacgaccg ttgtacggtg ctttttcgcc
      961 cagagcttct tcgatacgaa tcagctggtt gtatttagca acacggtcag aacggctcat
     1021 agaaccagtt ttgatctggc ctgcagcagt accaacagcc aggtcagcga tggtagcgtc
     1081 ttcagtttcg ccagaacggt gagagataac tgcagtgtag ccagcatctt tcgccatctt
     1141 gattgcagcc agagtttcgg tcagagaacc gatctggttg aatttgatca ggatggagtt
     1201 agcgatacct ttttcgatac cttctttcag gatcttggtg ttggttacga acaggtcgtc
     1261 accaaccagc tggattttgt cgcccagaac tttggtctgg tatgcgaaac cgtcccagtc
     1321 agattcgtcc agaccgtctt cgatagaaac gatcgggtac tgtttggtca gttcttccag
     1381 gaagtgagtg aattcttcag aggtgaacgc tttgttgcct tcgccagcca gaacgtattt
     1441 accgtctttg tagaattcag aagctgcgca gtccatcgcc agagtgatgt ctttgcccag
     1501 ttcataacca gcagctttaa cagcttcagc gataacagcc agagcttcag cgttggaacc
     1561 caggttcggc gcatagccac cttcgtcacc aactgcagtg ttcatgcctt tcgctttcag
     1621 aacttttgcc aggtgatgga aaacttcaga acccatgcgg atggcttctt tcacagtttt
     1681 cgcgccaacc ggctgaatca tgaattcctg gatatcaacg ttgttgtcag cgtgctcacc
     1741 accgttgatg atgttcatca tcggaaccgg catagagtat ttgcccggag taccgttcag
     1801 ttcagcgatg tgctcgtaca gcggcatacc tttagcagct gcagcagctt tggcgttagc
     1861 cagagataca gccaggattg cgttcgcgcc gaatttggat ttgttttcgg tgccgtccag
     1921 gtcgatcatg atcttgtcaa tgccagcctg atctttagca tctttgccaa tcagcgcctg
     1981 agcgatcggg ccgtttaccg cagcaacagc tttggttacg cctttaccca ggaaacggga
     2041 tttgtcgcca tcgcgcagtt ccagagcttc acgggaacca gtagaagcac ctgacggagc
     2101 agctgccata ccgacgaaac caccctccag atgtacttcg gcttcaacag tcgggttacc
     2161 acgggagtcg atgatttcac gaccgatgat ttttacgatt ttggacatta ggttttcctc
     2221 aagtcactag ttaaactgaa actccagaca aacaacgcgt acccagggta cgcgttgccg
     2281 ttctaacttt tttacttact tcgcctgacg tttctggaac tcgctggcgg ctttcacaaa
     2341 gcctgcaaac agcgggtgac catcacgtgg agtagaagta aactccggat ggaactggca
     2401 agccacgaac cacgggtgat tcggaacttc gatgatctcg accaactgat catccccgga
     2461 acggcccgca acgcgcagac ctgcatcttc aatctgtttc aacagcatgt tgttgacttc
     2521 gtaacggtga cgatgacgct caacaattgt cggcgcattg tacagctggc gaaccaggct
     2581 atcgtcaacc aactggcact gctgtgcgcc gagacgcatg gtaccgccga gatcgctctt
     2641 ctcgctacga acttcaacgt tgccgttttc atcgcgccac tcggtaatca gcgccacaac
     2701 cgggtactta cagtctggca caaattccgt agagttggcg ttctccatgt tggcaacatg
     2761 gcgagcgtaa tcaattaacg ccacctgcat acccaggcaa atgcccagat aaggaatatt
     2821 gttctcacgc gcaaaacgcg cggtcgtaat catgccttct acgccacgat agccgaaacc
     2881 gccaggtacg aggattgcgt ccagaccttt aaggatttca acgccgcgcg tttcaacatc
     2941 ttgtgaatcg atcagtttga tgttgacgct gacacgattc ttcagcccac cgtgtttcag
     3001 tgcttcgatc actgatttat aagcatccgg cagttcaatg tacttgccga ccataccgat
     3061 ggtgacttca cttaccgggt tcgcttcttc gaagataacc tgttcccatt cggacagatt
     3121 cgcttccggg cagtttaagc tgaatcgttt acaaatataa tcgtccagcc cctgagattt
     3181 caacaggccc ggaattttat agatggaatc gacgtctttc agagaaataa ccgctttttc
     3241 cggaacatta cagaacaatg caatcttcgc acgttcgttc gccggaacag cgcgatctga
     3301 acgacaaatc aggatgtcag gctggatacc gatggagagc agctctttta cagagtgctg
     3361 agtcggtttg gttttgactt caccagacgc tgccatgtac ggcaccagcg tcaggtgcat
     3421 aaacagagtg tgctcacggc caatttcaac agccatctgg cgaatcgctt cgaggaacgg
     3481 caaggattcg atatcaccta ctgtaccgcc gatttctacc agtactacgt catgaccttc
     3541 gccaccttcc agcacgcgct ctttgattgc gttagtgatg tgcggaataa cctgcacggt
     3601 tgcgccgagg tagtcaccgc ggcgttcttt acgcagaacg tcagagtaga tacgacccgt
     3661 ggtgaagttg ttgcggcggc tcattttggt acgaatgaaa cgctcgtagt gccccaggtc
     3721 caggtcggtt tcagcgccgt cttcagtaac gaacacttcc ccgtgttgga ttgggctcat
     3781 agtacctgga tcgacgttga tgtacggatc cagtttcatg atggtcacat tgaggccacg
     3841 ggcttcaaga atggctgcga gggaggctgc ggcaatgcct ttacccagag aggatacgac
     3901 cccgccggtc acaaaaatat agttcgttgt catgctgaac ctgagaagtt aggttgaaaa
     3961 gacgatggaa taaccaggac gggaaagcag tatacccgaa catgacctgt gccacaaact
     4021 ttcattatcc ctcctcttcg ccagcgcact attgaaatca ggagtgagaa aatagcccct
     4081 ttggggtaaa tgtttttgac gcaaatcaag cgcttgtcat ttaaaaaatg acacaaatgg
     4141 cgcttgaccg cgtaattccc ttagagatca atttcctgcc gttttacctg ttgccagact
     4201 tcttccattg tttcgaggtc aacacctgtc atttccagtc cacgcgcggc aacaatacgc
     4261 tccacttcgc gaaaacgacg ctcaaatttt tcgttcgctt tttgcaatgc gatttccgct
     4321 tttgtcccta aatgacgagc cagattgacc gtggcaaaca gcaggtcccc catttcctcc
     4381 tccagtttag cctggtcgac aacagcctgc cgcgcttcgt acatcacctc gtcgatctct
     4441 tcgtagactt tatcgactac cggaccaagc gtcgtccaat cgaagccaac gttggcgcaa
     4501 cgtttctgga ttttttgcgc acgcattaaa gccggtaaac tacgaggaat atcgtccagc
     4561 gccgaatgct gcgctttctg cgcgcgctct tcggttttga tttgctccca acgggcaagc
     4621 acttcactac tgttttcggc agaactatca gcaaaaacat gcggatggcg acgctctaat
     4681 ttatcgctaa tagcagcgca aatatcatta aagtcaaagc gcccttcttc ctgagccatt
     4741 tgcgcgtaaa acaccacctg gaatagcaga tcgcccagtt caccgcgaag atcgtcaaaa
     4801 tcttcacggg cgatggcgtc cagcacttcg taggtttctt caagggtgta aggcgcaatg
     4861 gtggcaaatg tctgctcttt atcccacggg cagccgtttt ccggatcgcg caggcgctgc
     4921 ataatagtga gcaaacggtc gatttgattc attgaattgt cctgaaaatt gcgggtctgt
     4981 caggtggaaa cctgtgacca gaatagaagt gagttagtaa cactacccaa tcagtacgtt
     5041 aattttggct ttaatgagtt gtaattcctc tggggcaact gttcctttct tcgttgctcc
     5101 tcttgcccgc caggcgatac tttttacctg atcagctaac gctacgccat cacgttcctg
     5161 accggataaa acaacttcga acggatatcc ttttgattgc gttgtataag gaacacacag
     5221 acacatacct gttttgttgt tgtacatgaa aggactcagg acaacagctg gacgatgtcc
     5281 agcttgctcg ctaccttttg tcgggtcaaa atcaacccaa atcagatcgc ccatatcggg
     5341 tacgtatcgg cttaccatta ccagacttcc ttatctttcg gctctcccca gtcgatattc
     5401 tcgtggaggt tttccggcgt gatgtcgttg accagttcag caagcgtaaa tacgggctct
     5461 ttacgcactg gctcaataat taatttgcca tccaccaggt caatcttcac ttcatcatca
     5521 atattcagat tgagcgcctg cattaacgta gccgggatcc gcaccgccgg tgaatttccc
     5581 caacgcttta cgctactgtg gatcataacc ctttcctcaa accgctatca tatgtagata
     5641 cagtatatat caatctacat tgtagatacg agcaaatttc ggcctaactc ccgtgcaacc
     5701 gacgcgcgtc gataacatcc ggcacctggt tgagtttacc cagcacgcgc cccagcactt
     5761 gcaggttgta aatctcaatg gtcatgtcga tggtcgccag ttgctgtttg gtgtcgctac
     5821 ggctggcaac gccaagcacg ttcaccttct cgttggcgag aatggtcgtg atatcacgta
     5881 acaacccact acgatcatta gccaccacgc ggaccaccag cgaatatccg gcggagtagc
     5941 tctcacccca taccgcgtca acaatgcgtt ctggcgcatg ggagcgcagt tccgccagtt
     6001 gttcgcaatc ggcgcggtgt actgaaatac cgcgcccctg ggtaatgaag ccgacaatct
     6061 catctccagg aatcggctgg cagcagcgcg cgatgtggtg catcaggttg ccaacacctt
     6121 cgaccaccac gcgaccgtta tctttactgc ggttttgcgg cgtgtagctt ttttgctgaa
     6181 gttgcttcag cgcggcggcg tcctgctctt cggcactcgg cttattaaat tgcgattgca
     6241 ggaagttcac catctgattg agacggatat ccccgccacc aatcgccgcc agcaactcgt
     6301 cgacatcatt aaagttgtaa cgcggcagca gatgtttttc tgcttctttc aggctgatcc
     6361 ccagatgttc cagctcgtcg tcaaggattt gccgcccagc cagaatgttt ttgtcacggt
     6421 cctgtttacg gaaccaggcg tgaattttcg aacgcccacg gctggttgtg acgtaaccga
     6481 ggtttgggtt taaccagtca cggctggggt tcggctgttt ctgggtgata atttcaatct
     6541 ggtcgcccat ctgcagctgg taggtgaacg gcacaatgcg cccgccaatt tttgccccga
     6601 tgcagcggtg tccgacatca ctgtggatgt ggtaagcgaa gtccagcggc gttgatcccg
     6661 caggcaaatc aacgacatca cctttcggcg taaagacata cacccggtcg tcaaagacct
     6721 ggctgcgtac ttcgtcgagc atttcgccgg aatcagccat ctcttcctgc cacgcaatca
     6781 gtttacgcag ccaggcaatc cggtcttcat gtcccgaacg tgcgccgcca gctgccgcgc
     6841 cctctttata tttccagtgc gcagcaacac ccaactctgc atcttcatgc atctgtttgg
     6901 tgcggatttg gatctcaacg gttttgccac ctggccccag aaccacggta tgaatagact
     6961 gataaccgtt tggtttcggg ttagcgacat aatcgtcaaa ctcatccggc aggtggcgat
     7021 agtgagtgtg cactatcccc agtgcggcat aacaatcctg taaacgctcg gcgacaatac
     7081 gtaccgcacg cacatcaaac agctcatcga aggcgaggtt ctttttctgc attttgcgcc
     7141 agatactgta gatgtgtttc ggacgaccat acacttcagc tttaacgcct tcagctttca
     7201 tctcagcgcg cagatggccg acaaactctt cgatatagtg ttcgcggtcg agacggcgtt
     7261 catgcagcag ttttgcgatg cgtttgtatt cggtcgggtg gagataacgg aagcagtaat
     7321 cttccagctc ccatttcagt tgcccaatcc caagacggtt cgccaacggc gcgtagatat
     7381 tggtgcactc ttttgccgcc agtacgcgtt catcttccgg cgcatctttt acttcgcgca
     7441 gatgagcaat acgctccgcc agtttgatga ccacgcagcg aaaatcatcg accatcgcca
     7501 ataacatccg gcgaacgtta tcgacctgtt cggaggaaac agaatcagtg tgcgtcgctt
     7561 tcagctggcg gatcgccgcc atatcacgca cgccgtgaat aaggttaacg accgacttac
     7621 cgacgctctc acgcagcaca tcttcgctga ctacgttggc atcagccaga gggaacagca
     7681 gcgccgcccg cagcgtgtca atgtccatac ttaatgtcga gaggatctcc accatctcaa
     7741 caccacgcca caataacaga ctggcatccg gatgcccctg cgtctgttgc agacaatacg
     7801 cccaggtttc ggctaagcac tcacacgact tctggctggt aatacccaga cttgcgatcc
     7861 atttttccgg atcaaattca ccagccttat tgatatgtgc acttcttacc gcaaccattg
     7921 tcctctcctt tagggaccag acctgccgaa atcggcaaat cgtaactatt taacgcgcga
     7981 gaaaagtacc atcgattcca gatgtcccgt gtgtgggaac atatccagca tcgccagtcg
     8041 cgcaatggta tatcctgctt ttaataacgc ttcgctatcc cgagccagcg ttgcagggtt
     8101 acaggataca taaactatac gaataggttc cagttttata atttgctgca taacacctgc
     8161 ggcacctgct cgcgccgggt ccagcaacac tttatcgaag ccgtttttcg cccacggctg
     8221 ctttgtgaca tcttcttcaa gattttcgtg ataaaacgtc acattctgta aaccattaag
     8281 acgcgcattc tgctggcctt tttccaccag cgccggaaca ccttctacac cgaccacact
     8341 ggctgcttgt ttcgccaatg gcagtgtaaa gttgcccata ccgcagaaca gatccagtac
     8401 gcgatcttca ggttgcacat ccagccattc caacgcacgc gctaccattt tttggttcac
     8461 acccgcattg acctgaataa aatcgcgcgg gctaaaagtt aagcgcaacc cgtttgagtc
     8521 ataccagggc atctcaccag agacggtttc gagtatctca ctatcggggg cgagatacag
     8581 atccaggcct tcagaatgcg aaaagcgttc cagtttttcg cgatctgccg aacttagcgg
     8641 tgcggtatgg cgcaaaatca tcagcgtgcc gctggttgcc tgtaccagtt caacatgacc
     8701 aagatggcgc ataacttgta agctgcccag gcatgccctg actttgggca gcaatgcttc
     8761 aagttggggc actaaaatgg ggcattgttt aacgtcgaca atgtcactgg agcccgcttt
     8821 gcgaaacccc atctgaagtt gctgtgtttt cggtaagtag ttcagactta aacgcgcgcg
     8881 gcggcgatag ccccagggaa catcggcgat cacttcagag acatcgtgtt tcattaatcg
     8941 ggcgagtgcc gcacttttgc ttcgctgctg taaatccacg ctggcgtgtt gttgctgaca
     9001 gccgccgcat acgccaaaat gaggacagcg tggtgtttcg cgttccgggc tatcgcttaa
     9061 ccggcgtacg actttagcgc gggcatactg ttttttatct tcagtaacag caacttccgc
     9121 gttttcctgc ggcaataatc cggggataaa taacgttttg ccgttatgtc gcgccacgcc
     9181 ctgaccaaaa gagtcgaggt cgttgactga aacggttatg atctgacgcg tcgtcgtgcg
     9241 tcgttttgca gagtagaatt gcgccattgg cgagactttc tcaatttaac agtgtgacct
     9301 taattgtccc ataacggaac tccatgacca actacagcct gcgcgcacgc atgatgattc
     9361 tgatcctggc accgaccgtc cttattggtt tattgctgag tatctttttc gtcgtgcatc
     9421 gctataacga cttgcagcgt caactggaag atgccggtgc cagcattatt gagccgcttg
     9481 cagtttctac tgaatatggc atgagcctgc aaaatcgcga atctatcggt cagttaataa
     9541 gcgtactgca tcgtcgccat tccgatattg ttcgcgcgat ttcggtttat gatgaaaata
     9601 accgactctt tgtcacctcc aattttcatc ttgatccctc atcaatgcag ctcggcagca
     9661 acgtgccgtt tcctcgccag ctcactgtca ctcgtgacgg cgatattatg atcctccgca
     9721 cgccgattat ttctgagagt tactcccccg acgaatcgcc cagtagcgat gccaaaaata
     9781 gtcaaaatat gttgggatat attgcgctgg agctggatct taaatcggtt cgcttgcagc
     9841 aatataaaga gatctttatt tccagcgtga tgatgctgtt ttgtatcggt attgcgctta
     9901 tttttggctg gcgcttaatg cgcgatgtaa ccggtccgat tcgcaacatg gtgaataccg
     9961 tcgaccgcat ccgtcgcggg caactcgaca gccgggtgga aggatttatg ctcggcgagc
    10021 tggatatgct gaaaaacggt atcaactcga tggcaatgtc gctggctgct tatcacgaag
    10081 agatgcagca caatatcgac caggcgacgt ccgatctgcg tgaaacgctg gagcagatgg
    10141 aaattcagaa cgttgagtta gatctggcga aaaagcgcgc ccaggaagcg gcgcgtatta
    10201 aatccgagtt tctggcaaat atgtcacacg agctgcgtac accactgaat ggtgttattg
    10261 gctttacccg cctgacgctg aaaacagaat taacaccaac gcagcgcgat cacctgaata
    10321 cgattgaacg ttcggcaaat aatttgctgg caattattaa tgatgttctc gacttctcga
    10381 aactggaagc aggtaagctg attctggaaa gtattccatt cccactacgc agcacgctgg
    10441 atgaagtcgt tactctgctg gcacattctt ctcacgataa agggttggaa ctgacgctca
    10501 atattaaaag cgacgtgcct gataacgtga tcggcgaccc actgcgatta cagcaaatca
    10561 tcactaacct ggtggggaat gcaattaaat tcaccgagaa tggcaacatt gatattctgg
    10621 tagaaaaacg tgcgctgagt aataccaaag tgcagattga agtgcagatt cgggataccg
    10681 gcattggtat tcctgaacgc gatcaatcgc gcttattcca ggccttccga caggctgatg
    10741 ccagtatttc ccgccgtcat ggtggcaccg gtctggggct ggtgattaca caaaaactgg
    10801 ttaatgaaat gggcggcgat atttcgttcc atagccagcc gaatcgcggt tcaactttct
    10861 ggttccacat taatctcgat ctgaacccga acattattat cgaagggcca tccacccagt
    10921 gcctcgccgg taagcgcctg gcctatgtcg aaccaaactc cgcagcagcg caatgcacgc
    10981 tggatatttt aagtgaaacg ccgctggaag tggtttatag cccaacgttc tccgcgctgc
    11041 ctcccgcgca ttacgacatg atgttgttag gcatcgcggt gaccttccgc gagccgctaa
    11101 caatgcaaca tgagcgatta gcgaaagcgg tatcgatgac cgatttcctg atgctggcac
    11161 ttccttgcca tgcacaagtc aatgcagaaa aactcaagca agatggtatc ggcgcgtgtc
    11221 tgctgaaacc attaacacct acgcgcctgt tgcctgccct gacggaattt tgtcatcaca
    11281 aacaaaacac gcttttgcct gtaaccgatg aaagcaagct ggcaatgaca gtcatggcag
    11341 ttgatgacaa ccccgctaac ctgaaactta tcggcgcatt gctggaagat atggtgcaac
    11401 atgtggaact ttgcgatagc gggcatcagg cggttgaacg ggcgaaacag atgccgttcg
    11461 atttgatctt aatggatatt caaatgcctg acatggatgg cattcgggcc tgcgagctca
    11521 tccaccagct cccccatcag caacaaacgc cggttatcgc ggtaacggcg catgcaatgg
    11581 ccgggcaaaa agagaagctg cttggcgcag ggatgagcga ttatctggcg aaaccgattg
    11641 aagaagagcg attgcataat ttgttgttgc gctacaagcc tggcagcggt atttcctctc
    11701 gcgtcgtgac gcccgaagtc aacgaaattg tggtgaaccc gaatgcgacc ctcgactggc
    11761 aactggcact acgccaggca gcaggaaaaa ccgatttagc gcgcgatatg ctgcaaatgt
    11821 tactcgattt cctgcctgaa gttcgcaaca aagttgagga acagctggtt ggagaaaacc
    11881 cggaaggcct ggttgatttg attcataaac tgcatggcag ttgcggctat agcggtgtgc
    11941 cgcgtatgaa gaatctctgc caactgatcg aacaacagct acgtagcggt actaaagaag
    12001 aagatttgga gccggagctg ctggaactgt tggacgagat ggataatgta gcgcgcgaag
    12061 ccagcaaaat tctcgggtaa cggacgtctt gtcgagtagc aatcggcttt ttatgacgct
    12121 ggtttcagat tggtgacaaa gtgcctgtgt ttatgccgga tgcggcgtta acgccttatc
    12181 cggcctacaa aaccaattaa attcaatgaa ttaaaaaatg atgtagacct gatacatagc
    12241 acaacaggct attttgcgtt tagcatcagt ctcaagccgg ctccagatag agccggtttt
    12301 ggttttctgt cttaacgcac catgcacggg cgcttgttat cgaacgtcca gccaggaatc
    12361 agatactgca ttcccatcgc atcgtcacgc gcgccaagcc cgtgtttctg atacagctca
    12421 tgggctttca tcacttgatc catatcgatt tctacaccca gccccggttt ttccggcacc
    12481 tgaaccagac cgcctttgat ctcaaacggt tctttggtca ggcgctgatt accttcctgc
    12541 cagatccagt gcgtatcaat agcagtaatt ttacccggtg cagcggcggc aacatgggta
    12601 aacatcgcca gggaaatatc gaagtggttg tttgagtgtg aaccccaggt caggccaaat
    12661 tcatggcaca tttgcgccac acgtaccgaa ccttgcatcg tccagaaatg cggatccgcc
    12721 agcgggatat caacggattg cagggagagc gtatggccca tctgccgcca gtcggtggcg
    12781 atcatattgg ttgcggtcgg caggccagtt gcgcgacgga actcagccat cacttcacgc
    12841 ccggagaaac cttgctccgc accacacgga tcttctgcat aagccagcga ccctttcagg
    12901 tatttaccaa ttttaatcgc ttcgttcagc gaccaggcac cgttaggatc gagcgtaata
    12961 cgcgcctgcg ggaagcgttg cgccagtgcc acaatagact cggcctcttc ttccccggcc
    13021 agtacaccgc ctttcagttt gaaatcgttg aagccatatt tttcatacgc cgcttccgcc
    13081 aggcgcacca ccgcatccgg cgtcatcgct tcttcatgac gcaggcgata ccagtcgcat
    13141 gagtcatccg gctggctttg atacggcagc ggtgtggctt tgcgatcacc gacgaagaac
    13201 agataaccga gcatttcgac ttcgctacgc tgttgaccat cgcccagcag cgatgccacg
    13261 tttaccccca gatgctgccc cagcagatcc agcattgccg cttctatccc ggtaactaca
    13321 tgaatagtgg tacgcaaatc aaatgtctgc aaaccgcgcc caccagcatc acgatcggca
    13381 aaagtattac gcaccagcgt cagaacgttt ttgtatttac ccagcgtttt acctaccacc
    13441 agcggaatcg catcttccag cgttttacgg attttctcgc cgccgggaat ttcccctacg
    13501 ccagtgtgac cagaattatc tttgataatc acaatattac gcgtaaagaa cggtgcgtgt
    13561 gcaccactca gattcatcag catactgtca tgacccgcca ccgggatgac ctgcatttca
    13621 gtaacaacag gcgtcgtaaa ttgagaactc atatttatgt cctttttcag aatcaatgac
    13681 ggccgaaaac gggacgttta cggtcaaagg tccagccggg gatcaggtac tgcatcggac
    13741 ctgcgtcgtt acgcgcaccg ccaggcagac gtttataggc ctcatgtgcc ttttgtacct
    13801 gttcccagtc cagttccacg cccagaccgg gcgcatcagg aacggcaatt tttccgtttt
    13861 taatctccag cggatttttg gtcaggcgac aatcgccctc ctgccaaatc cagtgggtat
    13921 cgatagcggt aggattaccc ggtgccgccg cgcccacatg ggtaaacatc gccagagaga
    13981 tatcgaaatg gttattagaa tggcagcccc aggtcagccc ccagtcgtcg caaagctgcg
    14041 ccacacggac tgcaccggaa agcgtccaga agtgcggatc ggcaagtgga atatctaccg
    14101 cattgagcat caccgcatga cccatttcgc gccagttggt ggcgatcatg ttagtcgcga
    14161 cgggcaagcc ggtcgcccgc cgaaactccg ccatcacttc acgaccggag aaaccctgtt
    14221 ctgcgccgca tggatcttcg gcataggtaa gaacatcatt cagccctttg catagagaaa
    14281 tggcttcatc aagtagccag gcaccgttgg gatcaacggt aatccgcgca tcagggaagc
    14341 gtttcttcaa tgcccgaaca gtgtcgattt cttgctcgcc aggtaacacc ccgcccttaa
    14401 gtttgaaatc tttaaagcca tagcgatcct gtgaggcttc cgccagacgc acaacggctt
    14461 cgctgttcat cgctttctga tggcgcaact gataccactc atggttgccc ggcgtatttt
    14521 ccagataagg aagatcggtt ttggtccgat caccgatata aaacagataa ccgaggacgg
    14581 taatagtctc gcgttgcttg cctggcccca acagttcgca gaccggaaca ttcagcgcct
    14641 tacctagcag gtcaagcaag gcggcttcca gcgccgccac ggcattaacg cgcaattcaa
    14701 aagtccaggc acctttgccg aaggtatcaa aatcggctgc ctgattacct ttatgcacct
    14761 gctgaaccac tttattcagg cgcgcaactt cctggcccag aaccatcgga atagcatcga
    14821 ccagtgtttg ataaatcacc tctccgcccg gcgcttcacc aatgccggta tgcccggcgt
    14881 tatcggtgag taccacaata ttgcgggtga aatatgcgtt atgtgcccca ccaatattaa
    14941 gcaacatgct atcgtgcccg gcgaccggaa tgactttcat atcagtaata acaggactgg
    15001 attgtgtcgt catcttattg ccccgcgaca ggtttcagct cgatacgctt gatatcgccc
    15061 accagcacca ggtagctcag taccgcgatt aaggcatgaa caccaacata aatcagcgcc
    15121 ccattaaagg agccagtcgt gccaacgata taaccaattg cgattggcgt gacgatgcca
    15181 gaaatgttac cgaacatgtt gaacaggcca ccggaaagac cgctgatctc ttttggcgcg
    15241 gtatctgcca ttactgccca acccagcgcc ccgatgccct taccgaagaa ggccagcgcc
    15301 ataaagccga tgatcatcca ctcaacgttg acgtagttgc agaacaccat caccatcgac
    15361 agcaacatgc ccattacgat cggtgtttta cgcgcaatgt tcagcgatcc cgtgcggcgc
    15421 atcagccagt cggaaataat cccacccagc acaccgccga taaaaccgca aaccgccgga
    15481 acggaagcca caaagcccgc tttcagaatc gacatcccac gcgcctgcac cagataaacc
    15541 ggaaaccagg taatgaaaaa gtaagtcagg gcgttgatac agtactgacc gatataaacg
    15601 ccgatcatca tccgtgaccc gagcagttgt ttgatctgcc cccacttcac gctgaacggc
    15661 actttaactt tggtgttttg ctgatccata ttgatcagcg cgccacccgc ggcgatgtac
    15721 tccagctctt tctgatttac ccccggatgt tgatttggct cgtggatgac tttcaaccag
    15781 ataaagctga tgacaatccc cagaccgccc ataaagaaga agacgtgtga ccagcccact
    15841 tcatgcgtca gccagcccat aatcggcgcg aagatcaccg ttgcgaagta ttgagcggag
    15901 ttaaaaatcg acaccgccgt tcccctttcc tgcgccggaa accaggccgc aacaatgcga
    15961 ctgttgccgg ggaaagatgg cgcttcagca agcccgacca ggaagcgcag cgtaaacagg
    16021 gcaacgataa tgccgaatcc actaaagata tcgacgaagc cttgcagcaa ggtaaacatc
    16081 gaccagataa agatcgacca gaagtagacg cgttttgaac caaaacgatc cagcaaccaa
    16141 ccaccaggga tctgcccgat aacataagcc catgagaagg cagagaacac atagcccatt
    16201 cccacgggat caaggccgat atctttggcc atttccgaac cagcaataga aagcgtagcg
    16261 cggtcgccgt agttgaagga tgtgacgata aacaacatca ccactatcca gtaacgagca
    16321 ttcgtgcgtt tttccacact gctcgcagcc tgacttaaag aactcattgt tgcactcctg
    16381 aaaattcgcg ttagccacgc tcactctgga ctgcgacatc gccaggaaat cagaggtgac
    16441 gtagggtgtt ttttgccgtt tttataggtc gttcgccgaa tacggcgcgt gtttatatct
    16501 ggcaatagca gtataaaaag cgcgccatag cggctcaccg tgcaacaaca caacattaat
    16561 gcgttcaatg aggcccgatt ttggcattag ccctggacgg tggaatccac ttcacggaaa
    16621 tgaaaacaag aacaagaaag gaagggtaaa aacgaagaaa taaagaagag tatgaaatgg
    16681 atcgcttgac tccaggcaaa cgccagtaaa aatccgcgct atgaagcagt ttttactggc
    16741 atttgcctga aaagattcga ttcagcaccg ctaaaacgac atttaccgct cgctgaacat
    16801 atcaggacaa cagcgtgccc cactgttcga cccacggatt tgattccgtt tccggttccg
    16861 ggttctcact ggcgtcaatc aacagtattt cgccaacccg ctgagcgctc tgttcctgca
    16921 acaaggcatc gaactgtttg ccgccattgc agaaatttac ataactgctg tcgccgagcg
    16981 caatcacgcc ataacgcaga ttcggctgga agcccagact atctttgatt ccctgaaaga
    17041 gcggcacaat gctatcagga aggtcgccct gcccggtcgt ggacgtaacc accagaacat
    17101 acttatcctg atagggcagc cagtcgctta attcaggatc ttcaaatacc gttgctttgt
    17161 ggccctgtgc ggtcagaatt gcttctgctt cttccgccac taacagcgaa tttccgtaca
    17221 tggtgccgac aaaaatacca atttccgcca taccgttttc tccctggatt aggaacttat
    17281 ctctccatcc tgacccgatg gcgcactgaa ctcaaccctt tcattttcag gaagcagacc
    17341 gcgccagcca aattgtgata acgcctgcat ccaggtgtcg tccaggcccg cgtgaatagt
    17401 cagcggctcg ccagtaaaag gatgcgtcaa tgacaactga ctggcatgta gcattaaccg
    17461 ctggaggcca aaatgctcag caccgctgcg attctggcgt aaatcgccat gtttgctatc
    17521 gccaataatc ggatgacgca aatgggcaag atgtcggcga agctgatgtt tgcgtccggt
    17581 tttcggctcc agttccacca ggccgtagcg cgtggtcggg taacgtccgg tcgctaccgg
    17641 catttctacg gtcgccagac cgcgataatg cgtcactgct ggctgcgagc ctttatcttc
    17701 gcgggcaaat ttatcagcga ttttgtccag ttcttccacc agtggataat ccagaactgc
    17761 ttcttccatc aaccagccgc gcacaatcgc atggtaacgt ttctggattt ggtgctgttc
    17821 aaactgttgt gccagcagcc gtccggcctc gctggataat cccatcaaca acacaccaga
    17881 agtgggtcgg tccagacgat gagcggtaaa aacgtgctgg ccaatctgat cacgcacggt
    17941 ttgcatgacc actactttct cgtcgcgatc cagccagctg cggtgaacca gccagccgga
    18001 gggtttattt accgcaacca gccattcatc ctgatagagt atttccagca ttagctcgca
    18061 tcatccgcaa aaagagcatc cagtttttcc agctcagcca gaataagcgc gcgttgcgga
    18121 tggtccgtcg ccagcgccat ttcataatag ggtgcaacgg caaaagcgcc cggtaacggc
    18181 tgtttgttat ctaacaaatc gtgcattcgc gggatcagca cccactgcaa ccactccagt
    18241 ggttccatgg tgtccataaa gaacggttgg gtactattaa attgatgcgg ctggggttca
    18301 tcgtttcgcc agtgctgatg ttcacgcagt aacgcttcaa gcgcctgcaa ctggagacga
    18361 acgcggtcat gagtggtcat aaagcaacct caataagaaa aactgccgcg aaggatagca
    18421 ggaataaaaa aagggagcac tgtattcaca gcgctcccgg ttcgtttcgc agcattccag
    18481 ctacttttgt tgctccctgc tcatccttga caacttttcc tctggccttg cggccaatcg
    18541 ttcatcctga actattgctt cctgctcaca ccaccccgat gtgatacttc atcctgaagt
    18601 gtccctggcc ttcctgaccc accgaatcat cctgaccggt tctcattctc catcctggag
    18661 gtgtccttta acgcgtcctg cgtcatcctc ttcgcttcat ccagaagcct ttccctgaaa
    18721 caccatcctg gtgtgtcctg cagaagtgtc atcatcctga tgttcacttc gttgtctgac
    18781 tccctgtcga cgaagataga atcgtctttt tcgcgaagtc ttacaaggcg cttacagaca
    18841 atgccttaag gaaaatttca tatgaaagtt aagaattaac aatcaacatt ttgattttaa
    18901 atgaaaataa cgattcttat cttttttagt attcgcttat ttcctggcga tctcctacag
    18961 agtctgtaag agatctctca caaccagatg gggtatttgg attacagaag aggcttaagt
    19021 tgattaagga attccgcaag atttgaggct aaatgcgtcc gtttacgtgt tcccagcgtc
    19081 tctttgcaca cttcaccgga gagattacac accgagatga cctctaactc ttcttccagc
    19141 gtcgcgataa atagcgtggg cggcagtttt agccttttct gagtcaccag atgaccaatc
    19201 agattttcct gcacccgacg gaagtcgtct tcactccagg tttgcagcaa tgtcagtttg
    19261 atatcgccaa actgcgcgtg catatccccg gcaaactgag tcgtataaaa ggtatgaatt
    19321 gtgggttgta tcacaatatc aaaagcgcgt tcaaccgcgt ttacattttg ttccccggta
    19381 aacggctggg gttgccagta tacggcatct tcggtagttg aaataatgca cggtgaagga
    19441 acaccgtaca gttcctcact taacggccag cttttatgct cttcatgcca cgcatcgcag
    19501 tagcgtgcag taaaatcttt cagggcttgt gcggtcaaat cgtccactaa tttctctctt
    19561 cacttaaacc agatacactt gtcgtttagt ttatctggtt tatgacggtg aaacatgtct
    19621 tcttatgcaa accatcaggc acttgcgggc ctgactcttg gaaaatcaac cgattaccgg
    19681 gatacctatg acgccaacct actgcaaggc gttccacgca gcctgaatcg cgacccgctg
    19741 ggtctgaaag cggataacct gccttttcac ggtacggata tctggacgct gtatgaactt
    19801 tcctggctga atgcgaaagg tttgccgcag gttgccgtcg gtcacgttga acttgattac
    19861 accagcgcaa atctgattga gtcgaagagt tttaagctct atctcaacag ttttaaccag
    19921 acgcgtttta ataactggga tgaggtgcgc cagacgctgg agcgcgactt aagcacttgc
    19981 gcccagggta aggttagcgt ggcgttatat cgtcttgatg aactggaagg ccagccgata
    20041 ggtcatttca atggcacctg cattgatgac caggatatca ctatcgataa ctatgaattc
    20101 actactgact atctggagaa tgccaccagc ggtgaaaaag tagtggaaga gacgcttgtc
    20161 agccacctgc tgaaatcaaa ctgcctgatc acccatcaac cagattgggg ttcgatccaa
    20221 attcagtatc gtggacgcca aattgacaga gaaaaactgc tgcgttacct ggtctcattc
    20281 cgtcatcaca acgagttcca cgaacagtgc gtggaacgca tctttaatga cctgttacgc
    20341 ttctgccagc cagagaaatt aagcgtttac gcacgttata cccgtcgtgg cggtctggac
    20401 attaacccgt ggcgcagtaa tagcgatttt gtcccatcga ccacaagact ggttcggcaa
    20461 taaatttttt ctcaattttg cgtgctggat tcacgcagaa ggttgtgaaa ggtcatcagg
    20521 cagggctatt gtaatcaaag ggaatgacga tattcgtccc ataaggagtt tttcttgatt
    20581 acacatatta gcccgcttgg ctccatggat atgttgtcgc agctggaagt ggatatgctt
    20641 aaacgcaccg ccagcagcga cctctatcaa ctgtttcgca actgttcact tgccgtactg
    20701 aactccggta gtttgaccga taacagcaaa gaattgctgt ctcgttttga aaatttcgat
    20761 attaacgtcc tgcgccgtga acgcggcgta aagctggaac tgattaatcc cccggaagag
    20821 gcttttgtcg atgggcgaat tattcgcgct ttgcaggcca acttgttcgc ggttctgcga
    20881 gacattctct tcgtttacgg gcaaatccat aataccgttc gttttcccaa cctgaatctc
    20941 gacaactccg tccacatcac taacctggtc ttttccatct tgcgtaacgc tcgcgcgctg
    21001 catgtgggtg aagcgccaaa tatggtggtc tgctggggcg gtcactcaat taacgaaaac
    21061 gagtatttgt atgcccgtcg cgtcggaaac cagctgggcc tgcgtgagct gaatatctgc
    21121 accggctgtg gtccgggagc gatggaagcg ccgatgaaag gtgctgcggt cggacacgcg
    21181 cagcagcgtt acaaagacag tcgttttatt ggtatgaccg agccgtcgat tatcgccgct
    21241 gaaccgccta acccgctggt caacgaattg atcatcatgc cagatatcga aaaacgtctg
    21301 gaagcgtttg tccgtatcgc tcacggtatc attatcttcc ctggcggtgt gggtacggca
    21361 gaagagttgc tgtatttgct gggaatttta atgaacccgg ccaacaaaga tcaggtttta
    21421 ccattgatcc tcaccggccc gaaagagagc gccgactact tccgcgtact ggacgagttt
    21481 gtcgtacata cgctgggcga aaacgcgcgc cgccattacc gcatcatcat tgatgacgcc
    21541 gctgaagtcg ctcgtcagat gaaaaaatcg atgccgctgg tgaaagaaaa tcgccgcgac
    21601 actggtgacg cttacagctt taactggtca atgcgcattg caccagattt gcaaatgccg
    21661 tttgagccgt ctcacgagaa tatggctaat ctgaagcttt acccggatca acctgttgaa
    21721 gtgctggctg ccgacctgcg ccgtgcgttc tccggtattg tggcgggtaa cgtaaaagaa
    21781 gtcggtattc gcgccattga agagtttggt ccttacaaaa tcaacggcga taaagagatt
    21841 atgcgtcgta tggacgacct gctacagggt tttgttgccc agcatcgtat gaagttgcca
    21901 ggctcagcct acatcccttg ctacgaaatc tgcacgtaat ctcccctccc ccggtgacgt
    21961 ttgttgccgg gttttctcgt ttttggtcac ttactcatca actcatttca tttgttatat
    22021 gaatgtttct taccaccctc acgcgacaaa tatcatca
//
LOCUS       DAZKHF020000058        21707 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2201S31241
            SAMN43267800-rid25106413.denovo.058, whole genome shotgun sequence.
ACCESSION   DAZKHF020000058 DAZKHF020000000
VERSION     DAZKHF020000058.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267800
            Sequence Read Archive: SRR30307263
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 21707)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21707)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 21707)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350581.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350581.2
            Genome Coverage       :: 151x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:21:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,652
            CDSs (total)                      :: 5,557
            Genes (coding)                    :: 5,320
            CDSs (with protein)               :: 5,320
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 237
            CDSs (without protein)            :: 237
            Pseudo Genes (ambiguous residues) :: 0 of 237
            Pseudo Genes (frameshifted)       :: 102 of 237
            Pseudo Genes (incomplete)         :: 130 of 237
            Pseudo Genes (internal stop)      :: 52 of 237
            Pseudo Genes (multiple problems)  :: 41 of 237
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21707
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267800-rid25106413.denovo.058"
                     /serotype="O 26:H11"
                     /isolate="2201S31241"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-01"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            80..808
                     /locus_tag="ACHOSC_RS08895"
                     /old_locus_tag="pgaptmp_001779"
     CDS             80..808
                     /locus_tag="ACHOSC_RS08895"
                     /old_locus_tag="pgaptmp_001779"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001049551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3251 domain-containing protein"
                     /protein_id="HGU4547736.1"
                     /translation="MNLKKIFFSAVTVSVLCALTGCDYIEEGKPESSLLKQQEEHNNK
                     IDLLEKQQAQLKSQLETIQKQQTGIISSTKTLTHVIKSVKDQQNTFIFTEFNPAKTKY
                     FILNNGSVALAGRVLSIDATENGSVIHISLVNLLSTPISNIGFNATWGGEKPVDAKEF
                     ARWQQLLFNTSMKSTLKLLPGQWQDINLTLKGVSPNNLGYLKLAINMENIQFDNLPSA
                     ENRQKRSKKINAVSTLITRNSEHH"
     gene            808..5658
                     /gene="ehaG"
                     /locus_tag="ACHOSC_RS08900"
                     /old_locus_tag="pgaptmp_001780"
     CDS             808..5658
                     /gene="ehaG"
                     /locus_tag="ACHOSC_RS08900"
                     /old_locus_tag="pgaptmp_001780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trimeric autotransporter adhesin EhaG"
                     /protein_id="HGU4547737.1"
                     /translation="MNKIFKVIWNPATGNYTVTSETAKSRGKKSGRSKLLISALVAGG
                     MLSSFGALANAGNDNGQGVDYGSGSAGDGWVAIGKGAKANTFMNTSGSSTAVGYDAIA
                     EGQYSSAIGSKTHAIGGASMAFGVSAISEGDRSIALGASSYSLGQYSMALGRYSKALG
                     KLSIAMGDSSKAEGANAIALGNATKATEIMSIALGDTANASKAYSMALGASSVASEEN
                     AIALGRSSVASGTDSLAFGRQSLASAANAIAIGAETEAAENATAIGNNAKAKGTNSMA
                     MGFGSLADKVNTIALGNGSQALADNAIAIGQGNKADGVDAIALGNGSQSRGLNTIALG
                     TASNATGDKSLALGSNSSANGINSVALGADSIADLDNTVSVGNSSLKRKIVNVKNGAI
                     KSDSYDAINGSQLYAISDSVAKRLGGGAAVDVDDGTVTAPTYNLKNGSKNNVGAALAV
                     LDENTLQWDQTKGKYSAAHGTSSPTASVITDVADGTISASSKDAVNGSQLKATNDDVE
                     ANTANIATNTSNIATNTANIATNTTNITNLTDSVGDLQADALLWNETKKAFSAAHGQD
                     TTSKITNVKDADLTADSTDAVNGSQLKTTNDAVATNTTNIANNTSNIATNTTNISNLT
                     ETVTNLGEDALKWDKDNGVFTAAHGTETTSKITNVKDGDLTTGSTDAVNGSQLKTTND
                     AVATNTTNIATNTTNISNLTETVTNLGEDALKWDKDNGVFTAAHGNNTASKITNILDG
                     TVTATSSDAINGSQLYDLSSNIATYFGGNASVNTDGVFTGPTYKIGETNYYNVGDALA
                     AINSSFSTSLGDALLWDATAGKFSAKHGTNGDASVITDVADGEISDSSSDAVNGSQLH
                     GVSSYVVDALGGGAEVNADGTITAPTYTIANADYDNVGDALNAIDTTLDDALLWDADA
                     GENGAFSAAHGKDKTASVITNVANGAISAASSDAINGSQLYTTNKYIADALGGDAEVN
                     ADGTITAPTYTIANAEYNNVGDALDALDDNALLWDETANGGAGAYNASHDGKASIITN
                     VANGSISEDSTDAVNGSQLNATNMMIEQNTQIINQLAGNTDATYIQENGAGINYVRTN
                     DDGLAFNDASAQGVGATAIGYNSVAKGDSSVAIGQGSYSDVDTGIALGSSSVSSRVIA
                     KGSRDTSITENGVVIGYDTTDGELLGALSIGDDGKYRQIINVADGSEAHDAVTVRQLQ
                     NAIGAVATTPTKYFHANSTEEDSLAVGTDSLAMGAKTIVNGDKGIGIGYGAYVDANAL
                     NGIAIGSNAQVIHVNSIAIGNGSTTTRGAQTNYTAYNMDAPQNSVGEFSVGSADGQRQ
                     ITNVAAGSADTDAVNVGQLKVTDAQVSQNTQSITNLNNQVTNLDTRVTNIENGIGDIV
                     TSGSTKYFKTNTDGVDANAQGKDSVAIGSGSIAAADNSVALGTGSVAEEENTISVGSS
                     TNQRRITNVAAGVNATDAVNVSQLKSSEAGGVRYDTKADGSIDYSNITLGGGNGSTTR
                     ISNVSAGVNNNDAVNYAQLKQSVQETKQYTDQRMVEMDNKLSKTESKLSGGIASAMAM
                     TGLPQAYTPGASMASIGGGTYNGESAVALGVSMVSANGRWVYKLQGSTNSQGEYSAAL
                     GAGIQW"
     gene            6027..7682
                     /gene="lldP"
                     /locus_tag="ACHOSC_RS08905"
                     /old_locus_tag="pgaptmp_001781"
     CDS             6027..7682
                     /gene="lldP"
                     /locus_tag="ACHOSC_RS08905"
                     /old_locus_tag="pgaptmp_001781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015129 - lactate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015727 - lactate transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-lactate permease"
                     /protein_id="HGU4547738.1"
                     /translation="MNLWQQNYDPAGNIWLSSLIASLPILFFFFALIKLKLKGYVAAS
                     WTVAIALAVALLFYKMPVANALASVVYGFFYGLWPIAWIIIAAVFVYKISVKTGQFDI
                     IRSSILSITPDQRLQMLIVGFCFGAFLEGAAGFGAPVAITAALLVGLGFKPLYAAGLC
                     LIVNTAPVAFGAMGIPILVAGQVTGIDSFEIGQMVGRQLPFMTIIVLFWIMAIMDGWR
                     GIKETWPAVVVAGGSFAIAQYLSSNFIGPELPDIISSLVSLLCLTLFLKRWQPVRVFR
                     FGDLGASQVDMTLAHTGYTAGQVLRAWTPFLFLTATVTLWSIPPFKALFASGGALYEW
                     VINIPVPYLDKLVARMPPVVSEATAYAAVFKFDWFSATGTAILFAALLSIVWLKMKPS
                     DAISTFGSTLKELALPIYSIGMVLAFAFISNYSGLSSTLALALAHTGHAFTFFSPFLG
                     WLGVFLTGSDTSSNALFAALQATAAQQIGVSDLLLVAANTTGGVTGKMISPQSIAIAC
                     AAVGLVGKESDLFRFTVKHSLIFTCMVGVITTLQAYVLTWMIP"
     gene            7682..8458
                     /gene="lldR"
                     /locus_tag="ACHOSC_RS08910"
                     /old_locus_tag="pgaptmp_001782"
     CDS             7682..8458
                     /gene="lldR"
                     /locus_tag="ACHOSC_RS08910"
                     /old_locus_tag="pgaptmp_001782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator LldR"
                     /protein_id="HGU4547739.1"
                     /translation="MIVLPRRLSDEVADRVRALIDEKNLEAGMKLPAERQLAMQLGVS
                     RNSLREALAKLVSEGVLLSRRGGGTFIRWRHDTWSEQNIVQPLKTLMADDPDYSFDIL
                     EARYAIEASTAWHAAMRATPGDKEKIQLCFEATLSEDPDIASQADVRFHLAIAEASHN
                     IVLLQTMRGFFDVLQSSVKHSRQRMYLVPPVFSQLTEQHQAVIDAIFAGDADGARKAM
                     MAHLSFVHTTMKRFDEDQARHARITRLPGEHNEHSREKNA"
     gene            8455..9645
                     /gene="lldD"
                     /locus_tag="ACHOSC_RS08915"
                     /old_locus_tag="pgaptmp_001783"
     CDS             8455..9645
                     /gene="lldD"
                     /locus_tag="ACHOSC_RS08915"
                     /old_locus_tag="pgaptmp_001783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinone-dependent L-lactate dehydrogenase"
                     /protein_id="HGU4547740.1"
                     /translation="MIISAASDYRAAAQRILPPFLFHYMDGGAYSEYTLRRNVEDLSE
                     VALRQRILKNMSDLSLETTLFNEKLSMPVALAPVGLCGMYARRGEVQAAKAADAHGIP
                     FTLSTVSVCPIEEVAPAIKRPMWFQLYVLRDRGFMRNALERAKAAGCSTLVFTVDMPT
                     PGARYRDAHSGMSGPNAAMRRYLQAVTHPQWAWDVGLNGRPHDLGNISAYLGKPTGLE
                     DYIGWLGNNFDPSISWKDLEWIRDFWDGPMVIKGILDPEDARDAVRFGADGIVVSNHG
                     GRQLDGVLSSARALPAIADAVKGDIAILADSGIRNGLDVVRMIALGADTVLLGRAFLY
                     ALATAGQAGVANLLNLIEKEMKVAMTLTGAKSISEITQDSLVQGLGKELPTALAPMAK
                     GNAA"
     gene            9831..10304
                     /gene="trmL"
                     /locus_tag="ACHOSC_RS08920"
                     /old_locus_tag="pgaptmp_001784"
     CDS             9831..10304
                     /gene="trmL"
                     /locus_tag="ACHOSC_RS08920"
                     /old_locus_tag="pgaptmp_001784"
                     /EC_number="2.1.1.-"
                     /EC_number="2.1.1.207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0001510 - RNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (uridine(34)/cytosine(34)/5-
                     carboxymethylaminomethyluridine(34)-2'-O)-
                     methyltransferase TrmL"
                     /protein_id="HGU4547741.1"
                     /translation="MLNIVLYEPEIPPNTGNIIRLCANTGFRLHIIEPMGFAWDDKRL
                     RRAGLDYHEFTAVTRHHDFRAFLEAENPQRLFALTTKGTPAHSAVNYQDGDYLMFGPE
                     TRGLPASILDALPAEQKIRIPMVPDSRSMNLSNAVSVVVYEAWRQLGYPGALLRD"
     gene            complement(10357..11178)
                     /gene="cysE"
                     /locus_tag="ACHOSC_RS08925"
                     /old_locus_tag="pgaptmp_001785"
     CDS             complement(10357..11178)
                     /gene="cysE"
                     /locus_tag="ACHOSC_RS08925"
                     /old_locus_tag="pgaptmp_001785"
                     /EC_number="2.3.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001112113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009001 - serine O-acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006535 - cysteine biosynthetic process
                     from serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine O-acetyltransferase"
                     /protein_id="HGU4547742.1"
                     /translation="MSCEELEIVWNNIKAEARTLADCEPMLASFYHATLLKHENLGSA
                     LSYMLANKLSSPIMPAIAIREVVEEAYAADPEMIASAACDIQAVRTRDPAVDKYSTPL
                     LYLKGFHALQAYRIGHWLWNQGRRALAIFLQNQVSVTFQVDIHPAAKIGRGIMLDHAT
                     GIVVGETAVIENDVSILQSVTLGGTGKSGGDRHPKIREGVMIGAGAKILGNIEVGRGA
                     KIGAGSVVLQPVPPHTTAAGVPARIVGKPDSDKPSMDMDQHFNGINHTFEYGDGI"
     gene            complement(11258..12277)
                     /gene="gpsA"
                     /locus_tag="ACHOSC_RS08930"
                     /old_locus_tag="pgaptmp_001786"
     CDS             complement(11258..12277)
                     /gene="gpsA"
                     /locus_tag="ACHOSC_RS08930"
                     /old_locus_tag="pgaptmp_001786"
                     /EC_number="1.1.1.94"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047952 - glycerol-3-phosphate
                     dehydrogenase [NAD(P)+] activity [Evidence IEA];
                     GO_process: GO:0006072 - glycerol-3-phosphate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent glycerol-3-phosphate
                     dehydrogenase"
                     /protein_id="HGU4547743.1"
                     /translation="MNQRNASMTVIGAGSYGTALAITLARNGHEVVLWGHDPEHIATL
                     ERDRCNAAFLPDVPFPDTLHLESDLATALAASRNILVVVPSHVFGEVLRQIKPLMRPD
                     ARLVWATKGLEAETGRLLQDVAREALGDQIPLAVISGPTFAKELAAGLPTAISLASTD
                     QTFADDLQQLLHCGKSFRVYSNPDFIGVQLGGAVKNVIAIGAGMSDGIGFGANARTAL
                     ITRGLAEMSRLGAALGADPATFMGMAGLGDLVLTCTDNQSRNRRFGMMLGQGMDVQSA
                     QEKIGQVVEGYRNTKEVRELAHRFGVEMPITEEIYQVLYCGKNAREAALTLLGRARKD
                     ERSSH"
     gene            complement(12277..12744)
                     /gene="secB"
                     /locus_tag="ACHOSC_RS08935"
                     /old_locus_tag="pgaptmp_001787"
     CDS             complement(12277..12744)
                     /gene="secB"
                     /locus_tag="ACHOSC_RS08935"
                     /old_locus_tag="pgaptmp_001787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015369180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-export chaperone SecB"
                     /protein_id="HGU4547744.1"
                     /translation="MSEQNNTEMTFQIQRIYTKDISFEAPNAPHVFQKDWQPEVKLDL
                     DTASSQLADDVYEVVLRVTVTASLGEETAFLCEVQQGGIFSIAGIEGTQMAHCLGAYC
                     PNILFPYARECITSMVSRGTFPQLNLAPVNFDALFMNYLQQQAGEGTEEHQDA"
     gene            complement(12807..13058)
                     /gene="grxC"
                     /locus_tag="ACHOSC_RS08940"
                     /old_locus_tag="pgaptmp_001788"
     CDS             complement(12807..13058)
                     /gene="grxC"
                     /locus_tag="ACHOSC_RS08940"
                     /old_locus_tag="pgaptmp_001788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin 3"
                     /protein_id="HGU4547745.1"
                     /translation="MANVEIYTKETCPYCHRAKALLSSKGVSFQELPIDGNAAKREEM
                     IKRSGRTTVPQIFIDAQHIGGCDDLYALDARGGLDPLLK"
     gene            complement(13200..13631)
                     /locus_tag="ACHOSC_RS08945"
                     /old_locus_tag="pgaptmp_001789"
     CDS             complement(13200..13631)
                     /locus_tag="ACHOSC_RS08945"
                     /old_locus_tag="pgaptmp_001789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HGU4547746.1"
                     /translation="MQEIMQFVGRHPILSIAWIALLVAVLVTTFKSLTSKVKVITRGE
                     ATRLINKEDAVVVDLRQRDDFRKGHIAGSINLLPSEIKANNVGELEKHKDKPVIVVDG
                     SGMQCQEPANALTKAGFAQVFVLKEGVAGWAGENLPLVRGK"
     gene            13876..15420
                     /gene="gpmM"
                     /locus_tag="ACHOSC_RS08950"
                     /old_locus_tag="pgaptmp_001790"
                     /gene_synonym="pgmI"
     CDS             13876..15420
                     /gene="gpmM"
                     /locus_tag="ACHOSC_RS08950"
                     /old_locus_tag="pgaptmp_001790"
                     /gene_synonym="pgmI"
                     /EC_number="5.4.2.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004619 - phosphoglycerate mutase activity
                     [Evidence IEA];
                     GO_process: GO:0006007 - glucose catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-bisphosphoglycerate-independent
                     phosphoglycerate mutase"
                     /protein_id="HGU4547747.1"
                     /translation="MSVSKKPMVLVILDGYGYREEQQDNAIFSAKTPVMDALWANRPH
                     TLIDASGLEVGLPDRQMGNSEVGHVNLGAGRIVYQDLTRLDVEIKDRAFFANPVLTGA
                     VDKAKNAGKAVHIMGLLSAGGVHSHEDHIMAMVELAAERGAEKIYLHAFLDGRDTPPR
                     SAESSLKKFEEKFAALGKGRVASIIGRYYAMDRDNRWDRVEKAYDLLTLAQGEFQADT
                     AVAGLQAAYARDENDEFVKATVIRAEGQPDAAMEDGDALIFMNFRADRAREITRAFVN
                     ADFDGFARKKVVNVDFVMLTEYAADIKTAVAYPPASLVNTFGEWMAKNDKTQLRISET
                     EKYAHVTFFFNGGVEESFKGEDRILINSPKVATYDLQPEMSSAELTEKLVAAIKSGKY
                     DTIICNYPNGDMVGHTGVMEAAVKAVEALDHCVEEVAKAVESVGGQLLITADHGNAEQ
                     MRDPATGQAHTAHTNLPVPLIYVGDKNVKAVEGGKLSDIAPTMLSLMGMEIPQEMTGK
                     PLFIVE"
     gene            15454..16713
                     /gene="envC"
                     /locus_tag="ACHOSC_RS08955"
                     /old_locus_tag="pgaptmp_001791"
     CDS             15454..16713
                     /gene="envC"
                     /locus_tag="ACHOSC_RS08955"
                     /old_locus_tag="pgaptmp_001791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418070.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein hydrolase activator EnvC"
                     /protein_id="HGU4547748.1"
                     /translation="MTRAVKPRRFAIRPIIYASVLSAGVLLCAFSAHADERDQLKSIQ
                     ADIAAKERAVRQKQQQRASLLAQLKKQEEAISEATRKLRETQNTLNQLNKQIDEMNAS
                     IAKLEQQKAAQERSLAAQLDAAFRQGEHTGIQLILSGEESQRGQRLQAYFGYLNQARQ
                     ETIAQLKQTREEVAMQRAELEEKQSEQQTLLYEQRAQQAKLTQALNERKKTLAGLESS
                     IQQGQQQLSELRANESRLRNSIARAEAAAKARAEREAREAQAVRDRQKEATRKGTTYK
                     PTESEKSLMSRTGGLGAPRGQAFWPVRGPTLHRYGEQLQGELRWKGMVIGASEGTEVK
                     AIADGRVILADWLQGYGLVVVVEHGKGDMSLYGYNQSALVSVGSQVRAGQPIALVGSS
                     GGQGRPSLYFEIRRQGQAVNPQPWLGR"
     gene            16717..17676
                     /locus_tag="ACHOSC_RS08960"
                     /old_locus_tag="pgaptmp_001792"
     CDS             16717..17676
                     /locus_tag="ACHOSC_RS08960"
                     /old_locus_tag="pgaptmp_001792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312519.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="divergent polysaccharide deacetylase family
                     protein"
                     /protein_id="HGU4547749.1"
                     /translation="MFPFRRNVLAFAALLALSSPVLAGKLAIVIDDFGYRPHNENQVL
                     AMPSAISVAVLPDSPHAREMATKAHNSGHEVLIHLPMAPLSKQPLEKNTLRPEMSSDE
                     IERIIRSAVNNVPYAVGINNHMGSKMTSNLFGMQKVMRALERYNLYFLDSVTIGNTQA
                     MRAAQGTGVKVIKRKVFLDDSQNEADIRVQFNRAIDLARRNGSTIAIGHPHPSTVRVL
                     QQMVYNLPPDITLVKASSLLNEPQVDTSTPPKNAVPDAPRNPFRGVKLCKPKKPIEPV
                     YANRFFEVLSESISQSTLIVYFQHQWQGWGKQPEAAKFNASAN"
     gene            complement(17663..18697)
                     /gene="waaH"
                     /locus_tag="ACHOSC_RS08965"
                     /old_locus_tag="pgaptmp_001793"
     CDS             complement(17663..18697)
                     /gene="waaH"
                     /locus_tag="ACHOSC_RS08965"
                     /old_locus_tag="pgaptmp_001793"
                     /EC_number="2.4.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucuronate:LPS(HepIII) glycosyltransferase"
                     /protein_id="HGU4547750.1"
                     /translation="MMNSTNKLSVIIPLYNAGDDFRTCMESLITQTWTALEIIIINDG
                     STDNSVEIAKHYAENYPHVRLLHQANAGASVARNRGIEVATGKYVAFVDADDEVYPTM
                     YETLMTMALEDDLDVAQCNADWCFRETGETWQSIPSDRLRSTGVLTGPDWLRMGLSSR
                     RWTHVVWMGVYRRDVIVKNNIKFIAGLHHQDIVWTTEFMFNALRARYTEQSLYKYYLH
                     NTSVSRLHRQGNKNLNYQRHYIKITRLLEKLNRNYADKITIYPEFHQQITYEALRVCH
                     AVRKEPDILTRQRMIAEIFTSGMYKRLITNVRSVKVGYQALLWSFRLWQWRDKTRSHH
                     RITRSAFNLR"
     gene            complement(18948..19973)
                     /gene="tdh"
                     /locus_tag="ACHOSC_RS08970"
                     /old_locus_tag="pgaptmp_001794"
     CDS             complement(18948..19973)
                     /gene="tdh"
                     /locus_tag="ACHOSC_RS08970"
                     /old_locus_tag="pgaptmp_001794"
                     /EC_number="1.1.1.103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005120462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008743 - L-threonine 3-dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006567 - threonine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonine 3-dehydrogenase"
                     /protein_id="HGU4547751.1"
                     /translation="MKALSKLKAEEGIWMTDVPVPELGHNDLLIKIRKTAICGTDVHI
                     YNWDEWSQKTIPVPMVVGHEYVGEVVGIGQEVKGFKIGDRVSGEGHITCGHCRNCRGG
                     RTHLCRNTIGVGVNRPGCFAEYLVIPAFNAFKIPDNISDDLASIFDPFGNAVHTALSF
                     DLVGEDVLVSGAGPIGIMAAAVAKHVGARNVVITDVNEYRLELARKMGITRAVNVAKE
                     NLNDVMAELGMTEGFDVGLEMSGAPPAFRTMLDTMNHGGRIAMLGIPPSDMSIDWTKV
                     IFKGLFIKGIYGREMFETWYKMAALIQSGLDLSPIITHRFSIDDFQKGFDAMRSGQSG
                     KVILSWD"
     gene            complement(19983..21179)
                     /gene="kbl"
                     /locus_tag="ACHOSC_RS08975"
                     /old_locus_tag="pgaptmp_001795"
     CDS             complement(19983..21179)
                     /gene="kbl"
                     /locus_tag="ACHOSC_RS08975"
                     /old_locus_tag="pgaptmp_001795"
                     /EC_number="2.3.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020239151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008890 - glycine C-acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006567 - threonine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine C-acetyltransferase"
                     /protein_id="HGU4547752.1"
                     /translation="MRGEFYQQLTNDLETARAEGLFKEERIITSAQQADITVADGSHV
                     INFCANNYLGLANHPDLIAAAKAGMDSHGFGMASVRFICGTQDSHKELEQKLAAFLGM
                     EDAILYSSCFDANGGLFETLLGAEDAIISDALNHASIIDGVRLCKAKRYRYANNDMQE
                     LEARLKEAREAGARHVLIATDGVFSMDGVIANLKGVCDLADKYDALVMVDDSHAVGFV
                     GENGRGSHEYCDVMGRVDIITGTLGKALGGASGGYTAARKEVVEWLRQRSRPYLFSNS
                     LAPAIVAASIKVLEMVEAGSELRDRLWANARQFREQMSAAGFTLAGADHAIIPVMLGD
                     AVVAQKFARELQKEGIYVTGFFYPVVPKGQARIRTQMSAAHTPEQITRAVEAFTRIGK
                     QLGVIA"
     gene            complement(21454..>21707)
                     /locus_tag="ACHOSC_RS08980"
                     /old_locus_tag="pgaptmp_001796"
     CDS             complement(21454..>21707)
                     /locus_tag="ACHOSC_RS08980"
                     /old_locus_tag="pgaptmp_001796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418075.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="WlaTC/HtrL family glycosyltransferase"
                     /protein_id="HGU4547753.1"
                     /translation="AIVGSQHKWKEFYKLVLESQKITLNNNIVDDDQGIFVMCYYKRP
                     DLFNLNYLGRGKWFDLFRCFRSNTLGAKMQALRIFLSRK"
BASE COUNT         5495 a         5643 c         5537 g         5032 t
ORIGIN      
        1 aagcaacata gatcaccaat aatacaatgg acaactctac cggcattgat cgctggtcgg
       61 aggaaataag gatttttaca tgaacttgaa gaagattttt ttcagtgctg tcactgtttc
      121 agttctctgc gcattaacgg gatgtgacta cattgaagaa ggcaagccag aaagtagttt
      181 attgaaacaa caagaagaac ataacaataa aattgatcta cttgaaaaac aacaagcaca
      241 gctaaagagt caactggaaa ctattcaaaa acaacaaact ggcataataa gcagtacaaa
      301 gactttaact cacgtaatta aatccgttaa agatcaacaa aacactttta tttttaccga
      361 gtttaaccca gcaaaaacga agtatttcat cctgaacaat ggttcggtgg ctttagcggg
      421 tcgagtgtta tccattgacg ccacagagaa tggcagtgtt attcatattt cactggtcaa
      481 cttattaagt acacctatct caaatattgg ttttaatgcg acatggggtg gtgaaaaacc
      541 tgtcgatgcc aaagagtttg cccggtggca acaattactt ttcaacacat caatgaaatc
      601 cacattgaaa ttattaccag gtcaatggca agacattaat ttgaccctga agggtgtatc
      661 gcccaataac ctgggatatc tgaagttagc catcaacatg gaaaatattc agttcgacaa
      721 tcttccttct gctgaaaatc ggcagaaaag aagcaaaaaa ataaatgcag ttagtacgct
      781 tataacaagg aatagcgagc atcattaatg aacaaaatat ttaaagttat ctggaaccct
      841 gcgacaggga attatactgt taccagcgaa acggcaaaaa gccgtggcaa gaaatctggg
      901 cgcagtaagc tgttaatttc tgcgctggtt gcgggtggaa tgttgtcgtc gtttggggca
      961 ttggcgaatg ccgggaatga caacggtcag ggtgttgatt acggtagtgg atcagctggc
     1021 gacggctggg ttgctatagg caaaggggcg aaagcaaata cttttatgaa caccagtggt
     1081 tccagtactg ctgtgggtta tgacgctata gctgaaggcc aatatagctc tgccatcggg
     1141 tcaaaaaccc atgcgattgg tggtgcatca atggcctttg gggttagtgc aatatcagaa
     1201 ggcgatagaa gtatagcact gggtgcctct tcgtattcat tgggccaata ctcaatggcc
     1261 ctcggccgtt attcaaaagc attgggtaaa ttgtctattg ctatggggga ctcttccaaa
     1321 gcggaaggag caaacgccat tgccctggga aatgccacta aagctactga gattatgagt
     1381 attgctcttg gcgacaccgc caatgcgtca aaagcgtatt caatggcgct gggagcaagt
     1441 agcgtcgcat ctgaagaaaa cgcaattgcc ctggggcgta gcagtgtagc tagcggtact
     1501 gacagcctcg catttggcag acaatcactt gccagcgcag cgaacgctat tgcgataggt
     1561 gctgagaccg aagccgctga aaatgcaact gctattggca ataatgcgaa ggcaaaaggg
     1621 actaatagca tggcaatggg gttcggaagc cttgccgata aagtcaatac tatcgcatta
     1681 ggaaatggca gccaggctct ggcagataat gcaatcgcca taggccaggg caacaaagct
     1741 gatggcgtgg atgccatcgc tctgggtaat ggtagccagt cgagaggctt aaacaccatt
     1801 gccttaggca cagccagtaa tgcaactggt gataagagtc ttgcgcttgg tagtaatagc
     1861 agtgccaacg gtattaactc tgtcgcgctg ggcgcagatt ccattgcgga tttagacaat
     1921 accgtctctg tcggcaatag ttcattaaaa cgcaagatcg ttaatgtgaa aaatggcgcg
     1981 atcaagtctg acagttacga tgccattaat ggttcacagc tttatgccat tagcgactcg
     2041 gtagcaaaaa ggcttggagg aggggctgca gtagatgttg atgacggtac tgttacagca
     2101 ccaacctaca atttaaaaaa tggtagcaaa aataacgtag gggctgcgct cgctgtactt
     2161 gatgaaaaca ccctgcaatg ggaccaaacc aaaggcaaat acagcgctgc tcatggtact
     2221 agtagcccaa ctgccagcgt aatcaccgat gttgcggatg gcacgatttc agcctccagt
     2281 aaggatgcgg ttaacggttc ccaactgaaa gctaccaatg acgatgtcga agccaacacc
     2341 gccaatatcg ctactaatac cagcaacatt gccacgaata cggcaaatat tgccaccaat
     2401 accaccaata tcaccaacct gacggattcc gttggtgacc ttcaggctga tgccctgctc
     2461 tggaacgaaa ctaaaaaggc attcagtgca gctcacggcc aggataccac cagcaaaatc
     2521 accaacgtta aagatgccga cctgacggct gacagcactg atgctgttaa cggctctcag
     2581 ctgaaaacca ccaacgatgc tgtggcgacg aataccacca atatcgccaa taacacttcc
     2641 aatattgcca ctaacaccac caacatctct aacctgactg agacggtgac taatcttggt
     2701 gaggatgcgc tgaaatggga taaggacaat ggtgtattca cggcagctca tggcaccgag
     2761 accaccagca aaatcaccaa cgttaaagat ggcgacctga cgactggcag caccgatgcc
     2821 gttaacggct ctcagctgaa aaccaccaac gatgccgtgg cgacgaatac caccaatatc
     2881 gccactaaca ccaccaacat ctctaatctg actgagacgg tgactaatct tggtgaggat
     2941 gcgctgaaat gggataagga caatggtgtc ttcactgcag ctcatggcaa caataccgcc
     3001 agcaaaatca ccaatatcct ggacggcaca gtcactgcaa ccagttccga tgccattaac
     3061 ggtagccagc tttatgactt aagcagcaat atcgccacct acttcggcgg caatgcttct
     3121 gtgaatactg acggtgtgtt taccggtcca acctacaaaa tcggtgaaac aaattattat
     3181 aacgtcggcg atgcactggc tgcgattaac tcctcattta gcacgtctct cggcgatgct
     3241 ctgctttggg atgccaccgc aggtaaattc agtgccaaac acggtactaa tggtgacgca
     3301 agcgtgatca ctgatgtcgc agatggtgaa atttcagact ccagttctga cgcagtaaac
     3361 ggctcacaac tccacggcgt gagcagttat gttgttgatg cgctgggggg tggtgccgaa
     3421 gtcaatgcag acggcaccat cactgcgccg acgtacacca ttgctaatgc tgattacgat
     3481 aatgtcggtg atgccctgaa tgctatcgat accactcttg acgacgctct gctctgggat
     3541 gcggacgccg gtgaaaatgg tgcatttagc gccgctcacg gaaaagataa aactgccagt
     3601 gtaatcacta acgtcgctaa cggtgcaatc tctgctgcca gcagcgacgc gattaacggc
     3661 tcacaactct ataccaccaa taagtacatc gctgatgcgc tgggtggtga cgcagaagtc
     3721 aacgctgacg gcaccatcac cgcaccgact tacaccattg cgaacgccga gtacaacaac
     3781 gtcggtgacg ccctggatgc gcttgatgat aacgccttgc tgtgggatga gactgccaat
     3841 ggcggtgctg gagcctacaa tgccagccat gacggtaaag ccagcatcat cactaatgtc
     3901 gctaatggca gtattagtga ggacagtacc gatgcagtga acggttctca gttgaatgcg
     3961 acgaatatga tgattgagca gaacacccaa attatcaatc agctcgctgg taacaccgac
     4021 gcaacctata tccaagaaaa cggtgcgggt attaactatg tgcgtactaa cgacgacggc
     4081 ttagcgttca acgacgccag cgcacagggt gttggcgcta cagctatagg ttataactct
     4141 gtcgccaaag gcgatagcag cgtagctatt ggtcagggca gctacagcga cgttgatacg
     4201 ggtatcgccc tgggtagcag ctctgtttcc agccgagtga ttgccaaagg ctcccgtgac
     4261 accagcataa cggaaaatgg cgttgttatt ggttacgaca ccacggatgg cgaactgctc
     4321 ggtgcattgt ctatcggtga tgacggtaaa tatcgtcaaa tcatcaacgt agccgatggt
     4381 tccgaagccc atgacgccgt tacggttcgt caattgcaga atgcgattgg tgcggtcgca
     4441 accacgccga ctaaatactt ccacgctaat tcaacggaag aagattcact ggcagtggga
     4501 actgactcgc tggcaatggg tgcgaaaacc atcgtgaatg gcgataaagg tattggtatc
     4561 ggttatggtg cctacgtgga cgcgaatgca cttaacggca ttgccattgg tagcaatgcg
     4621 caagtcattc atgtcaacag tattgcgata ggtaatggtt ctacgaccac tcgtggcgct
     4681 caaaccaatt ataccgccta caacatggac gcaccgcaga actctgtcgg tgaattctca
     4741 gtcggtagtg cggatggtca acgtcagatc actaacgtcg cagcaggttc ggctgatacc
     4801 gatgcggtca acgtgggtca gttgaaagta acggatgcgc aggtttccca gaatacccag
     4861 agcattacta acctgaacaa tcaggtcact aatctggata ctcgcgttac taatatcgaa
     4921 aacggtattg gcgacattgt cacctccggt agcaccaagt acttcaagac caacaccgat
     4981 ggcgtagatg ccaacgccca gggtaaagat agcgttgcta ttggttctgg ttccattgct
     5041 gccgctgaca acagcgtcgc actgggtacc ggttccgttg cagaggaaga aaatacaatc
     5101 tctgtaggtt cttctactaa ccaacgccgt atcaccaacg tagctgccgg tgttaatgcc
     5161 accgatgcgg ttaacgtgtc gcagctgaaa tcttctgaag caggcggcgt gcgctacgac
     5221 accaaagctg atggttctat cgactatagc aatatcaccc tcggtggtgg caacggcagt
     5281 acgactcgta tcagcaacgt ctccgctggc gtcaacaaca acgacgcggt gaactacgcg
     5341 cagttgaagc aaagcgtgca ggaaacgaag caatacaccg atcagcggat ggttgagatg
     5401 gataacaaac tgtctaaaac tgaaagcaag ttgagcggtg gtatcgcttc tgcaatggca
     5461 atgaccggtc tgccgcaggc ttatacaccg ggtgccagca tggcttctat tggtggcggt
     5521 acttacaacg gtgaatcggc agttgcttta ggtgtatcga tggtgagcgc caatggtcgt
     5581 tgggtctaca aattacaagg tagtaccaat agccagggtg aatactccgc cgcactcggt
     5641 gccggtattc agtggtaatg atccattaag ttagtgtgac taagggcaaa cgccaaattg
     5701 cctgatgcgt tacgcgcatc aggcaagata aggcgctccg ggtcaacaat ctaagggcaa
     5761 ttctctgatg aggattgccc ttttctttac cagacatctc ccccccacaa gaattggccc
     5821 taccaattct tcgcttatct gacctctggt tcacaattcc ccaattaaaa ctcacatcaa
     5881 tattgccaat acataacatt tagttaacca ttcattgtca ttatccctac acaacacaat
     5941 tggcagtgcc acttttacac aacgtgtgac aaggagatga gcaacagact cattacacga
     6001 tgtgcgtgga ctccaggaga cctgcaatga atctctggca acaaaactac gatcccgccg
     6061 ggaatatctg gctttccagt ctgatagcat cgcttcccat cctgtttttc ttctttgcgc
     6121 tgattaagct caaactgaaa ggatacgtcg ccgcctcatg gacggtagca atcgcccttg
     6181 ccgtggcttt gctgttctat aaaatgccgg tcgctaacgc gctggcctcg gtggtttatg
     6241 gcttcttcta tggtttgtgg cccatcgcgt ggatcattat tgcagcagtg ttcgtctata
     6301 agatctcggt gaaaaccggg cagtttgaca tcattcgctc gtctattctt tcgataaccc
     6361 ctgaccagcg tctgcaaatg ctgatcgtcg gtttctgttt cggcgctttc cttgaaggag
     6421 ccgcaggctt tggcgcaccg gtagcaatta ccgccgcatt gctggtcggc ctgggtttta
     6481 aaccgctgta cgccgccgga ctgtgcctga ttgttaacac cgcgccagtg gcatttggcg
     6541 cgatgggcat tccaatcctg gttgccggac aagtaacagg catcgacagt tttgagattg
     6601 gccagatggt ggggcgtcag ctaccgttta tgaccattat cgtgctgttc tggatcatgg
     6661 cgattatgga cggctggcgc ggtatcaaag agacgtggcc tgcggtcgtg gttgcgggcg
     6721 gctcgtttgc catcgctcag taccttagct ccaacttcat tgggccggag ctgccggaca
     6781 ttatctcttc gctggtatca ctgctctgcc tgacgctgtt cctcaaacgc tggcaaccag
     6841 ttcgcgtatt ccgcttcggt gacttggggg cgtcacaggt tgatatgacg ctggcccaca
     6901 ccggttacac cgcgggtcag gtgttacgtg cctggacacc gttcctgttc ctgaccgcta
     6961 ccgtaacact gtggagcatc ccgccgttta aagccctgtt cgcatcgggt ggtgcgctgt
     7021 atgagtgggt gatcaacatt ccggtgccgt acctcgataa actggttgcc cgtatgccac
     7081 cagtggtcag cgaggctaca gcctatgccg ccgtgtttaa gtttgactgg ttctctgcca
     7141 ccggcaccgc cattctgttt gctgcactgc tctcgattgt ctggctgaag atgaaaccgt
     7201 ctgacgctat cagcaccttc ggcagcacgc tgaaagaact ggctctaccc atctactcca
     7261 tcggcatggt gctggcgttc gcctttatct cgaactattc cggactgtca tcaacactgg
     7321 cactggcact ggcgcacacc ggtcatgcat tcaccttctt ctcgccgttc ctcggctggc
     7381 tgggggtatt cctgaccggg tcggatacct catctaacgc cctgttcgcc gcgctgcaag
     7441 ccaccgcagc acaacaaatt ggcgtctctg atctgttgct ggttgccgcc aataccaccg
     7501 gtggcgtcac cggtaagatg atctccccgc aatctatcgc tatcgcctgt gcggcggtag
     7561 gcctggtggg caaagagtct gatttgttcc gctttactgt caaacacagc ctgatcttca
     7621 cctgtatggt gggcgtgatc accacgcttc aggcttatgt cttaacgtgg atgattcctt
     7681 aatgattgtt ttacccagac gcctgtcaga cgaggttgcc gatcgtgtgc gggcgctgat
     7741 tgatgaaaaa aacctggaag cgggcatgaa gttgcccgct gagcgccaac tggcgatgca
     7801 actcggcgta tcacgtaatt cactacgcga ggcgctagca aaactggtga gcgaaggcgt
     7861 gctgctcagt cgacgcggcg gcgggacgtt tattcgctgg cgtcatgaca catggtcgga
     7921 gcaaaacatc gtccagccgc taaaaacgct gatggccgat gatccggatt acagtttcga
     7981 tattctggaa gcccgctacg ccattgaagc cagcaccgca tggcatgcgg caatgcgcgc
     8041 cacacctggc gacaaagaaa agattcagct ttgctttgaa gcaacgctaa gtgaagaccc
     8101 ggatatcgcc tcacaagcgg acgttcgttt tcatctggcg attgccgaag cctcacataa
     8161 catcgtgctg ctgcaaacca tgcgcggttt cttcgatgtc ctgcaatcct cagtgaagca
     8221 tagccgtcag cggatgtatc tggtgccacc ggttttttca caactgaccg aacaacatca
     8281 ggctgtcatt gacgccattt ttgctggtga tgccgacggg gcgcgtaaag caatgatggc
     8341 gcaccttagt tttgttcaca ccaccatgaa acgattcgat gaagatcagg ctcgccacgc
     8401 acggattacc cgcctgcccg gtgagcataa tgagcattcg agggagaaaa acgcatgatt
     8461 atttccgcag ccagcgatta tcgcgccgca gcgcaacgca ttctgccgcc gttcctgttc
     8521 cactatatgg atggtggtgc atattctgaa tacacgctgc gccgcaacgt ggaagatttg
     8581 tcagaagtgg cactgcgcca gcgtattctg aaaaacatgt ccgacttaag cctggaaacg
     8641 acgctgttta atgagaaatt gtcgatgccg gtagcactgg caccggtggg tttgtgtggc
     8701 atgtatgcgc gtcgtggcga agttcaggca gccaaagcgg cggacgcgca tggcattccg
     8761 tttactctct cgacggtttc cgtttgcccg attgaagaag tcgcgccagc catcaagcgc
     8821 ccaatgtggt tccagcttta tgtactgcgc gatcgcggct ttatgcgtaa cgcgctggag
     8881 cgagcaaaag cagcgggttg ttcgacgctg gttttcaccg tggatatgcc gacaccgggc
     8941 gcacgctacc gtgatgcgca ttcaggtatg agcggcccaa atgcggcaat gcgccgctac
     9001 ttgcaggcgg tgacgcatcc gcaatgggcg tgggatgtgg gcctgaacgg tcgtccacat
     9061 gatttaggta atatctcagc ttatctcggc aaaccgaccg gactggaaga ttacatcggc
     9121 tggctgggga ataacttcga tccgtccatc tcatggaaag accttgagtg gatccgcgat
     9181 ttctgggatg gcccgatggt gatcaaaggg atcctcgatc cggaagatgc gcgcgatgca
     9241 gtacgttttg gtgctgatgg aattgtggtt tctaaccacg gtggccgcca gctggacggt
     9301 gtactctctt ccgctcgtgc gttgcccgct attgcagatg cggtgaaagg tgatatcgcc
     9361 attctggcgg atagcggaat tcgtaacggg cttgatgtcg tgcgtatgat tgcgctcggt
     9421 gccgacaccg tactgctggg tcgtgctttc ctgtatgcgc tggcaacagc gggccaggcg
     9481 ggtgtagcta acctgctaaa tctgatcgaa aaagagatga aagtggcgat gacgctgact
     9541 ggcgcgaaat cgatcagtga aattacgcaa gattcgctgg tgcaggggct gggtaaagag
     9601 ttgcctacgg cactggctcc gatggcgaaa gggaatgcgg catagtcgtt tgcgccccct
     9661 caccctaacc ctctccctca gggagagggg accgttcggc gctgtatgta ctccctcact
     9721 ctgaaacgac accgcactct ttttttctcc ctcgcccctc cggggtgagg ggaaaaggcc
     9781 gcactgctca ccaattttct gctatcctgc ccccgcacta agggggcagt atgctaaaca
     9841 tcgtacttta cgaaccagaa attccgccaa atactggcaa tatcatccgt ctttgcgcta
     9901 ataccggctt tcgtctgcat atcatcgaac cgatgggatt tgcctgggac gataagcgcc
     9961 tgcgccgcgc ggggctggac tatcacgagt ttaccgccgt tacgcgtcat catgactttc
    10021 gcgcgttcct cgaagcagaa aatccccagc gcctgtttgc cctcactacg aaaggtacgc
    10081 ctgctcacag cgccgtaaac tatcaggatg gcgattatct gatgttcggc ccggaaacac
    10141 gcggcctgcc agcgagcatt cttgatgccc tgcccgctga acaaaaaatt cgcattccaa
    10201 tggtgccgga cagccgcagc atgaacctgt ccaatgcggt gtcggtagtg gtgtatgaag
    10261 cctggcggca gctgggatat ccgggagcgt tgttgagaga ttagttactg tatgccggat
    10321 agatgattac atcgcatccg gcacgatcac agcacatcag atcccatccc catactcaaa
    10381 tgtatggtta ataccgttga aatgctggtc catatccatt gatggcttat cgctgtctgg
    10441 tttaccgaca atacgagccg gaacaccagc ggcggtagta tgcggcggca ccggttgcag
    10501 caccacggaa cctgcgccaa tcttcgcgcc gcgaccaact tcaatattgc cgagaatttt
    10561 cgcgcccgcg ccaatcatca caccttcacg aattttcggg tgacggtcac caccagattt
    10621 acccgtaccg ccaagcgtca cagattgcag aatcgatacg tcgttttcaa ttaccgccgt
    10681 ttcaccaacg acgatgcccg tggcgtggtc gagcatgata ccgcgaccaa tttttgctgc
    10741 cgggtgaata tcgacctgga acgtcacaga aacctggttt tgcagaaaga ttgccagtgc
    10801 gcgacgcccc tgattccaca accagtgacc gatgcgatag gcctgcaagg catgaaaacc
    10861 cttcaggtat aacaacgggg ttgagtattt atcgactgcc gggtcgcggg tacgcaccgc
    10921 ctgaatatca caggccgcag aggcgatcat ttccgggtca gcggcgtagg cttcttcaac
    10981 cacttcacgg atagcaatag caggcataat tggcgatgac agcttgttcg ccagcatgta
    11041 gctcagggca ctgccaaggt tttcgtgctt gagtagcgtc gcgtggtaaa aactggccag
    11101 cattggctca cagtccgcca gcgttctggc ttcggcttta atattgttcc agacaatttc
    11161 cagttcttca cacgacattg cttactccac acgatgagat aatgaccggc ccgttctgcg
    11221 cgggccgggt catagcggta acaaaggttc cctggggtta gtggctgctg cgctcgtcct
    11281 tgcgtgcacg acctagtaaa gtcaatgctg cctcgcgcgc gttttttccg caatataata
    11341 cttgataaat ttcctcggtt attggcattt caacgccgaa gcgatgcgcc agttcgcgga
    11401 cttctttcgt attgcggtag ccttccacca cctgaccaat cttctcctgc gcgctttgta
    11461 catccatgcc ctgaccgagc atcatgccaa aacggcggtt acgcgactgg ttgtcggtac
    11521 aggtaagcac cagatcgccc agccccgcca tgcccataaa ggtggcagga tcggcaccca
    11581 gcgccgcacc aagacgcgac atttcagcca gcccacgggt gatcagcgcc gtacgcgcat
    11641 tcgcaccaaa accgataccg tcagacatcc ccgcgccaat ggcaatgacg tttttcaccg
    11701 cgccgccaag ctgcacgcca atgaaatccg ggttgctgta aacgcggaaa cttttgccac
    11761 agtgcagcaa ttgttggaga tcatcggcaa aggtctggtc ggtagaggcc aacgaaatag
    11821 ccgtcggtaa acctgccgcc agttctttcg caaacgttgg gccagagata accgccagcg
    11881 gaatttgatc gcctaaggcc tcacgcgcca cgtcctgtaa cagacgtccg gtttccgctt
    11941 ccagcccttt ggtcgcccac accagacgcg catcaggacg catcagcggt ttaatctggc
    12001 gcagcacttc accaaagaca tggctgggta cgacgacgag aatattacgg ctggctgcca
    12061 gcgcagtggc gagatcgctt tcaagatgga gcgtatcggg aaaaggcaca tcggggagaa
    12121 acgcggcgtt acagcggtcg cgttcaagcg ttgcgatatg ttcagggtca tggccccaga
    12181 ggacaacctc gtggccattt cttgccaggg taatggcaag agcggtgccg tacgagccgg
    12241 caccgatcac agtcattgaa gcattacgtt ggttcatcag gcatcctgat gttcttcagt
    12301 accttcgcca gcctgctgct gcaaatagtt catgaacagc gcatcgaagt taaccggcgc
    12361 aaggttcagt tgcgggaatg taccgcggga taccatgctg gtgatgcact cacgagcata
    12421 cgggaacaga atgttcgggc agtatgcgcc caggcaatgt gccatctggg tgccttcgat
    12481 acccgcgatg gagaaaatac cgccctgctg aacttcacac aggaacgcgg tttcttcgcc
    12541 cagggaggcc gttacggtaa cacgcagtac cacttcgtat acgtcatctg ccagttggga
    12601 agatgccgta tccagatcaa gtttaacttc tggttgccaa tctttctgga aaacgtgcgg
    12661 cgcgttcggc gcttcgaaag agatatcctt ggtataaata cgttggatct ggaaagtcat
    12721 ttcagtgttg ttttgttctg acatgtgtag aaaaccctta agtgttgtcc ttaaatactg
    12781 cgcaatgtaa tgccaacgtt cacacgttat ttcagcaggg gatccagtcc accacgtgca
    12841 tccaatgcat acaagtcatc acagccgcca atgtgctgtg cgtcaataaa aatctgggga
    12901 accgtggtgc gaccgctgcg tttgatcatc tcttcacgct tggcggcatt gccatcgatc
    12961 ggcaactcct gaaaactcac gcccttgctg ctcagcagtg cttttgcacg atggcaatac
    13021 gggcaggttt ctttggtata gatttcaaca ttggccataa tttagctcct gtttttttac
    13081 cctgaaattt catgttgcag ggcggctgca aaatggagtg tccccagaag cttataacaa
    13141 taagtcgctg ggggcctaat tgcagctaac ggcctgcatc atgaaagacg acaggtaaat
    13201 tatttgccgc gcaccagagg caagttttcg ccagcccagc cagcaacgcc ctctttcagc
    13261 acgaatactt gcgcaaaacc agctttcgtc agcgcgtttg caggctcctg gcactgcatg
    13321 ccagaaccgt ctaccacgat aaccggtttg tctttgtgct tctcaagctc accaacattg
    13381 ttggctttga tttcactcgg caacagattg atagaacctg cgatatggcc tttacggaag
    13441 tcatcacgct gacgtaaatc cacaaccaca gcgtcttctt tgttgatcag acgcgtagct
    13501 tcaccacgag taatcacctt cactttcgag gtcaggctct taaacgtagt cacaagaacc
    13561 gccaccagta acgcgatcca ggcgatactc agtatgggat gacggccaac aaattgcata
    13621 atttcttgca tggggggtaa caactcccga cgtagtgatt aaaaaaacca ggaaaggagt
    13681 atacctgcgc ggtgcggcaa atacagccag cgcgttaact ggaatgcaat tttgcggggc
    13741 acgacgaaaa aaaagcgcaa atctggcatc ggcttgccgc gagcggtcgt atattttgat
    13801 cttcagaggc tattttatcg attcagctgt agtaaaatta cgcaaatttt gactcttgag
    13861 tatgaggttg tcgcaatgtc ggtttctaaa aaacctatgg tactggtgat tctggatggc
    13921 tatggctatc gtgaagaaca gcaggataac gccattttta gtgctaaaac cccggtaatg
    13981 gatgcactgt gggccaatcg tccgcatacc ctgatcgacg cttccggtct ggaagtcggt
    14041 ctgcctgacc gtcagatggg taactccgaa gtaggccacg ttaacctggg tgccggccgc
    14101 atcgtgtatc aggacctgac tcgtctggac gttgaaatca aagatcgcgc tttctttgct
    14161 aatccagtgc tgactggcgc ggtagataaa gcgaaaaacg caggtaaagc agtacacatt
    14221 atgggtctgc tctccgcagg cggcgtacac agccacgaag atcacatcat ggcgatggta
    14281 gaactggcag ctgaacgcgg cgcagaaaaa atctacctgc acgcattcct tgacggtcgc
    14341 gacaccccgc cgcgcagtgc tgaatcctcg ctgaaaaaat ttgaagagaa atttgctgcg
    14401 ctgggtaaag gccgcgtagc gtccatcatt ggtcgttact acgcgatgga ccgcgataac
    14461 cgttgggatc gcgttgaaaa agcctatgac ctgctgactc tggcgcaggg tgagttccag
    14521 gccgatactg ccgttgctgg tttgcaggca gcttatgctc gcgacgaaaa cgacgagttc
    14581 gtgaaagcga ccgttattcg tgcagaaggt cagccagatg cggcaatgga agacggcgat
    14641 gcgctgattt tcatgaactt ccgtgctgac cgcgcgcgtg aaatcactcg tgcattcgtg
    14701 aacgctgatt tcgatggctt cgcgcgtaag aaagtggtta acgtcgattt cgtgatgctg
    14761 accgaatacg ccgctgacat caaaactgcg gttgcttacc cacccgcttc cctggttaac
    14821 accttcggcg agtggatggc gaaaaacgac aaaactcagt tgcgtatttc cgaaaccgaa
    14881 aaatatgccc acgttacttt cttcttcaac ggtggcgtag aagagtcgtt caaaggcgaa
    14941 gatcgcattc tgatcaactc accgaaagtg gctacctacg atctgcaacc ggaaatgagc
    15001 tccgcagagc tgaccgaaaa actggttgcg gccatcaaga gcggcaaata cgacaccatt
    15061 atctgtaact atccgaacgg cgacatggta ggtcacaccg gggtgatgga agcggcggtt
    15121 aaagcggttg aagcactgga tcactgcgtg gaagaagtcg cgaaagcggt tgagtccgtg
    15181 ggtggacaac tgctgatcac cgctgaccac ggtaacgctg aacagatgcg cgatccggca
    15241 acgggtcagg cacacacggc acacaccaac ctgccagttc cgctgattta tgttggtgat
    15301 aagaacgtga aagcggttga aggcggcaaa ctttctgaca tcgcgccgac catgttgtcg
    15361 ctgatgggta tggaaatccc gcaagagatg actggtaagc cgctgttcat cgtggaataa
    15421 tccctcccca tgaggggaaa ggcgattaat accatgacac gggccgtgaa accgcgcagg
    15481 tttgcaatca ggcccatcat ctacgccagc gttcttagcg ctggcgtatt gttgtgcgcc
    15541 ttttccgccc acgcggatga gcgtgaccaa ctcaaatcta ttcaggccga tatcgccgca
    15601 aaagagcgcg cggtacgcca aaagcaacaa caacgcgcaa gcctgctcgc acaattgaaa
    15661 aagcaggaag aagcgatctc tgaagccacc cgtaagctgc gcgaaacgca aaacacgctc
    15721 aatcaactca ataaacagat tgatgagatg aacgcgtcga ttgccaaact ggagcagcaa
    15781 aaagccgccc aggagcgcag cctcgccgcg caactggatg ccgcattccg tcagggcgag
    15841 cataccggta ttcagctaat tctcagcggt gaagaaagcc agcgtggaca gcgtttacag
    15901 gcttatttcg gctatctcaa ccaggcgcga caagaaacca ttgcccagtt gaagcaaacg
    15961 cgtgaagaag tcgccatgca gcgtgctgaa ctggaagaga aacaaagcga gcaacaaacg
    16021 ctgttatatg aacagcgcgc ccaacaggcg aaactgactc aggctctgaa cgagcgtaaa
    16081 aagacgctgg cggggctgga atcttccatc cagcaaggtc agcagcagct aagtgaactg
    16141 cgtgccaacg aatcccgtct gcgtaacagc attgcccgtg cggaagccgc ggcgaaagcg
    16201 cgtgctgaac gagaagcgcg tgaggcccag gcggttcgcg accgccagaa agaagcgacg
    16261 cgcaaaggca ccacctataa accgaccgaa agcgaaaaat cgctgatgtc ccgaactggt
    16321 ggcctgggtg cgccgcgtgg tcaggcattc tggccggttc gcgggccgac gctgcatcgc
    16381 tatggtgaac agctacaggg cgaactacgc tggaaaggaa tggttatcgg tgcctctgaa
    16441 ggtactgaag ttaaagcgat tgccgacggt cgggtgattc tggctgactg gctgcaaggt
    16501 tacggtctgg tggtggtggt tgagcacggt aaaggcgaca tgagtcttta cggctataat
    16561 cagagcgcac tggtgagcgt tggttcgcag gttcgcgcgg gccagccaat tgcactggtg
    16621 ggcagcagtg gcggtcaggg tcggccttca ctctatttcg aaattcgccg ccagggtcag
    16681 gcggtcaatc cacagccgtg gttgggaaga taagttttgt ttccatttcg tcgtaacgtt
    16741 cttgcatttg ccgctctgtt ggcgctctcc tcccccgtac ttgctggcaa acttgccatc
    16801 gtcattgatg attttgggta tcgcccgcac aacgaaaacc aggtgctggc gatgccctcc
    16861 gctatctccg tcgctgtatt acccgattca ccgcacgcca gagagatggc gaccaaagcg
    16921 cataacagcg ggcacgaagt gttgattcat ctcccgatgg caccactgag taaacagccg
    16981 ctggagaaaa atacgctgcg cccggagatg agcagcgacg aaattgagcg cattattcgt
    17041 agtgcggtca ataacgtgcc ctatgccgtg gggatcaaca accacatggg tagcaagatg
    17101 acctctaacc tgtttggtat gcagaaagtg atgcgggcac tggagcgtta caatctttac
    17161 ttcctcgaca gcgtaaccat cggtaatacc caggcgatgc gcgccgcgca aggtactggc
    17221 gtgaaggtga tcaaacggaa ggtgttcctc gacgattcgc agaacgaagc ggacatccgt
    17281 gtgcaattta atcgcgcaat tgatctggcg cgtcgcaacg gttcaaccat tgccattggg
    17341 catcctcacc cttcaacggt acgcgtgcta caacagatgg tttataacct gccgccagac
    17401 attacgctgg tgaaagccag cagcttgctg aatgaaccgc aggttgatac ttctacaccg
    17461 ccgaaaaacg ctgtgcctga tgcaccgcgt aatccgttcc gtggcgtgaa gctgtgcaaa
    17521 ccgaagaaac cgatagaacc tgtttatgct aatcgcttct ttgaagtatt aagcgaaagc
    17581 atcagccaga gcacgctgat cgtttacttc cagcatcagt ggcaaggctg gggcaaacag
    17641 cccgaagcgg cgaagtttaa cgctagcgca aattaaaggc gctacgcgta atgcgatggt
    17701 gcgaccgcgt tttgtcgcgc cattgccata agcggaaaga ccacagtaac gcctggtaac
    17761 cgaccttcac gctgcgcaca ttggtaatca ggcgcttata cataccggaa gtaaatatct
    17821 cggcaatcat ccgttggcgg gtaagaatat ccggctcttt gcgcaccgca tggcaaacac
    17881 gcaatgcttc gtaagttatt tgctgatgaa attccggata aatcgtaatt ttgtcggcat
    17941 aatttcgatt taatttctcc agcaggcggg taatcttaat atagtgacgt tgataattaa
    18001 ggtttttatt cccttgtcta tgcaaccgac tcactgacgt attatgcaga taatatttat
    18061 ataatgattg ctcggtatat cgcgcacgca gcgcgttaaa catgaattct gttgtccaga
    18121 caatatcctg atgatgtaat ccggcaataa atttaatgtt atttttaaca ataacatcac
    18181 ggcgataaac ccccatccag acaacgtgag tccaacggcg cgaagaaagc cccatccgca
    18241 gccagtccgg gccggttaat acgccggttg agcgaaggcg atcgctgggg atggattgcc
    18301 aggtttctcc cgtttcacga aaacaccagt cagcgttgca ctgcgccacg tcgaggtcat
    18361 cctctaacgc catggtcatc agcgtttcgt acatggtggg atagacctca tcgtcagcat
    18421 cgacaaaagc gacatatttg cccgtcgcca cctcgatccc acgattacgc gctaccgatg
    18481 cgccagcatt cgcctgatgc aacaaacgaa cgtgcggata gttttctgcg taatgctttg
    18541 ctatttcaac agaattatcc gttgaaccat cgttaataat aatgatttcc agagcagtcc
    18601 aggtttgcgt aattaaagat tccatacaag tgcggaaatc atcgcccgca ttatataacg
    18661 gaataataac actaagttta ttggtgctgt tcatcataaa ttaccgacga cctctgaagt
    18721 tatcctgacg ttttacaggc tttgtattaa gaaactatta agcctgtgag atattatttt
    18781 tacaattatt tttacaggtg agagagtcac ttcagggtag agagataatg agagcagcta
    18841 tataagagat cagcgcaatt acgatatctt tattacactg acaggatttt acatatttaa
    18901 cggattatcc tcaggcaaaa gggctggaat accagccctt gttcgtgtta atcccaactc
    18961 agaattactt tcccggactg gcccgaacgc atagcgtcaa agcccttctg gaaatcatcg
    19021 atagagaaac gatgggtaat gatcggcgag agatccagac ctgactgaat cagcgccgcc
    19081 atcttgtacc aggtttcaaa catctcacga ccgtaaatac ctttaatgaa caagccttta
    19141 aagattactt tggtccagtc gatagacata tcggacggtg gaatacccag catcgcaata
    19201 cgaccgccgt ggttcatggt atcgagcatg gtacgaaacg ctggcggcgc gccggacatt
    19261 tccagaccga catcaaagcc ttcggtcatg cccagttcag ccatcacatc attaaggttt
    19321 tctttcgcga cgttaaccgc acgggtgata cccattttgc gcgccagctc aaggcggtat
    19381 tcgttaacat cggtgatcac cacattgcgt gcaccaacgt gtttcgccac cgccgcagcc
    19441 ataataccta tcggacctgc accagaaacc agcacatctt cgcccaccag atcgaacgac
    19501 agcgcggtat gcacggcgtt accgaaggga tcaaaaatgg aagccaggtc gtcggagata
    19561 ttgtctggga ttttgaaggc gttgaacgcc gggatcacca gatattcggc gaagcagccc
    19621 ggacggttaa cgccgacgcc gatcgtgttg cggcacagat gggtacgacc gccacgacag
    19681 ttgcgacagt gaccgcaggt gatatggcct tcgccagaaa cgcgatcgcc gattttgaag
    19741 cctttcactt cctgaccaat acctaccact tcaccgacat attcatggcc tacgaccatc
    19801 ggcaccggga tggttttttg cgaccactca tcccagttat agatgtgaac gtcagtcccg
    19861 cagatggctg ttttacggat tttaatcagc agatcgttat gcccgagttc cggtacagga
    19921 acgtcggtca tccagatgcc ctcttccgct ttcagtttgg ataacgcttt catctcacat
    19981 cctcaggcga taacgcccag ttgtttacca atacgcgtga atgcttctac tgcacgcgta
    20041 atttgctcag gggtatgcgc cgcagacatc tgggtacgaa tacgcgcctg acctttcgga
    20101 acgaccggat agaagaaacc ggtgacgtaa atgccctctt tttgcagctc acgggcaaat
    20161 ttctgcgcca ctactgcatc gccaagcatg accggaataa tggcatgatc ggctcccgcc
    20221 agggtaaagc ccgccgccga catttgctca cggaactggc gcgcgttcgc ccacagacgg
    20281 tcacgcagtt cgctgcccgc ttcgaccatc tccagtactt tgatggacgc ggcaacaatg
    20341 gccggtgcca gcgagttgga gaacaggtac ggacgagaac gctggcgcag ccactcaacc
    20401 acctctttgc gcgccgcggt ataaccacca gaagccccgc ccagcgcttt accaagcgta
    20461 ccggtgataa tatcgacccg gcccatcaca tcgcagtatt catgggaacc acgaccattt
    20521 tcaccgacaa aaccgaccgc gtgggagtcg tctaccatca ccagggcatc gtatttatct
    20581 gccagatcgc aaacgccctt cagattggca atcacgccgt ccatcgagaa tacaccatcg
    20641 gtggcaatca gcacatgacg cgcaccggct tcacgcgctt ctttcagacg agcttcaagc
    20701 tcctgcatat cgttgttggc atagcgatag cgtttagctt tacacagacg cacaccatca
    20761 ataatagacg cgtggttcag tgcgtcggag ataatggcgt cttccgcgcc cagcagcgtt
    20821 tcaaacaggc caccgttagc atcaaagcag gaagagtaga gaatcgcatc ttccatcccc
    20881 aggaaggccg ccagtttttg ttcaagctct ttatggctgt cctgagtgcc gcaaataaaa
    20941 cgcaccgaag ccatgccgaa accgtgagaa tccattcccg cctttgccgc cgcaatcaga
    21001 tcaggatgat tcgccagccc gagatagttg ttggcacaaa agttaatgac gtggcttcca
    21061 tcagccacag tgatatctgc ttgctgcgca gacgtaataa tgcgctcttc tttaaacaac
    21121 ccttccgccc gtgcggtttc cagatcgttg gttaactgct gataaaattc tccacgcatt
    21181 gcgattctcc agacagggca aattccagca catattaccc aaacttatag gtcgcgacga
    21241 gatgacgcgt taacacttct gcaaaattca ggataaataa cgtgtaactt gatgtctctc
    21301 aacacctaca gatagtagaa aaactgtaca gttttggctg ggcataatga tgattgattt
    21361 tactgcattg atataaaaag aaaaaatgat gccaaagcat catttttcct atctcagttg
    21421 gtatcaaaac gctacccact aatggaggga aacctatttt cttgaaagga aaattctcag
    21481 cgcctgcatt tttgccccta aagtgttact cctgaagcag cgaaacaaat cgaaccattt
    21541 tcctctcccc agatagttta ggttgaagag atcggggcgt ttgtaatagc acatgacaaa
    21601 aatgccctgg tcatcatcca caatgttatt atttagtgtt attttttgac tttccagaac
    21661 taatttatag aattctttcc atttatgctg tgaaccaaca attgcgc
//
LOCUS       DAZKHF020000059        21520 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2201S31241
            SAMN43267800-rid25106413.denovo.059, whole genome shotgun sequence.
ACCESSION   DAZKHF020000059 DAZKHF020000000
VERSION     DAZKHF020000059.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267800
            Sequence Read Archive: SRR30307263
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 21520)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21520)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 21520)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350581.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350581.2
            Genome Coverage       :: 151x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:21:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,652
            CDSs (total)                      :: 5,557
            Genes (coding)                    :: 5,320
            CDSs (with protein)               :: 5,320
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 237
            CDSs (without protein)            :: 237
            Pseudo Genes (ambiguous residues) :: 0 of 237
            Pseudo Genes (frameshifted)       :: 102 of 237
            Pseudo Genes (incomplete)         :: 130 of 237
            Pseudo Genes (internal stop)      :: 52 of 237
            Pseudo Genes (multiple problems)  :: 41 of 237
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21520
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267800-rid25106413.denovo.059"
                     /serotype="O 26:H11"
                     /isolate="2201S31241"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-01"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            complement(<1..218)
                     /locus_tag="ACHOSC_RS08985"
                     /old_locus_tag="pgaptmp_001797"
     CDS             complement(<1..218)
                     /locus_tag="ACHOSC_RS08985"
                     /old_locus_tag="pgaptmp_001797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4547754.1"
                     /translation="MSGKPAARQGDMTQYGGSIVQGSAGVRIGAPTGVACSVCPGGVT
                     SGHPVNPLLGAKVLPGETDIALPGPLPF"
     gene            complement(421..822)
                     /gene="nikR"
                     /locus_tag="ACHOSC_RS08990"
                     /old_locus_tag="pgaptmp_001798"
     CDS             complement(421..822)
                     /gene="nikR"
                     /locus_tag="ACHOSC_RS08990"
                     /old_locus_tag="pgaptmp_001798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016151 - nickel cation binding [Evidence
                     IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0010045 - response to nickel cation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nickel-responsive transcriptional regulator
                     NikR"
                     /protein_id="HGU4547755.1"
                     /translation="MQRVTITLDDDLLETLDSLSQRRGYNNRSEAIRDILRSALAQEA
                     TQQHGTQGFAVLSYVYEHEKRDLASRIVSTQHHHHDLSVATLHVHINHDDCLEIAVLK
                     GDMGDVQHFADDVIAQRGVRHGHLQCLPKED"
     gene            complement(828..1634)
                     /gene="nikE"
                     /locus_tag="ACHOSC_RS08995"
                     /old_locus_tag="pgaptmp_001799"
     CDS             complement(828..1634)
                     /gene="nikE"
                     /locus_tag="ACHOSC_RS08995"
                     /old_locus_tag="pgaptmp_001799"
                     /EC_number="7.2.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015413 - ABC-type nickel transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015675 - nickel cation transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nickel import ATP-binding protein NikE"
                     /protein_id="HGU4547756.1"
                     /translation="MTLLNISGLSHHYAHGGFNGKHQHQAVLNNVSLTLKSGETVALL
                     GRSGCGKSTLARLLVGLESPAQGNISWRGEPLAKLNRAQRKAFRRDIQMVFQDSISAV
                     NPRKTVREILREPMRHLLSLKKSEQLARASEMLKAVDLDDSVLDKRPPQLSGGQLQRV
                     CLARALAVEPKLLILDEAVSNLDLVLQAGVIRLLKKLQQQFGTACLFITHDLRLVERF
                     CQRVMVMDNGQIVETQVVGDKLTFSSDAGRVLQNAVLPAFPVRRRTTEKV"
     gene            complement(1631..2395)
                     /gene="nikD"
                     /locus_tag="ACHOSC_RS09000"
                     /old_locus_tag="pgaptmp_001800"
     CDS             complement(1631..2395)
                     /gene="nikD"
                     /locus_tag="ACHOSC_RS09000"
                     /old_locus_tag="pgaptmp_001800"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0015413 - ABC-type nickel transporter
                     activity [Evidence IEA];
                     GO_function: GO:0016151 - nickel cation binding [Evidence
                     IEA];
                     GO_process: GO:0015675 - nickel cation transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nickel import ATP-binding protein NikD"
                     /protein_id="HGU4547757.1"
                     /translation="MPQQIELRNIALQAAQPLVHGVSLTLQRGRVLALVGGSGSGKSL
                     TCAATLGILPAGVRQTAGEILADGKPVSPCALRGIKIATIMQNPRSAFNPLHTMHTHA
                     RETCLALGKPADDATLTAAIEAVGLENAARVLKLYPFEMSGGMLQRMMIAMAVLCESP
                     FIIADEPTTDLDVVAQARILDLLESIMQKQAPGMLLVTHDMGVVARLADDVAVMSDGK
                     IVEQGDVETLFNAPKHTVTRSLVSAHLALYGMELAS"
     gene            complement(2395..3228)
                     /gene="nikC"
                     /locus_tag="ACHOSC_RS09005"
                     /old_locus_tag="pgaptmp_001801"
     CDS             complement(2395..3228)
                     /gene="nikC"
                     /locus_tag="ACHOSC_RS09005"
                     /old_locus_tag="pgaptmp_001801"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015099 - nickel cation transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nickel ABC transporter permease subunit NikC"
                     /protein_id="HGU4547758.1"
                     /translation="MNFFLSSRWSVRLALIIIALLALIALTSQWWLPYDPQAIDLPSR
                     LLSPDAQHWLGTDHLGRDIFSRLMTATRVSLGSVMACLLLVLTLGLVIGGSAGLIGGR
                     VDQATMRVADMFMTFPTSILSFFMVGVLGTGLTNVIIAIALSHWAWYARMVRSLVISL
                     RQREFVLASRLSGAGHVRVFVDHLAGAVIPSLLVLATLDIGHMMLHVAGMSFLGLGVT
                     APTAEWGVMINDARQYIWTQPLQMFWPGLALFISVMAFNLVGDALRDHLDPHLVTEHA
                     H"
     gene            complement(3225..4169)
                     /gene="nikB"
                     /locus_tag="ACHOSC_RS09010"
                     /old_locus_tag="pgaptmp_001802"
     CDS             complement(3225..4169)
                     /gene="nikB"
                     /locus_tag="ACHOSC_RS09010"
                     /old_locus_tag="pgaptmp_001802"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nickel ABC transporter permease subunit NikB"
                     /protein_id="HGU4547759.1"
                     /translation="MLRYVLRRFLLLIPMVLAASVIIFLMLRLGTGDPALDYLRLSNL
                     PPTPEMLASTRTMLGLDQPLYVQYGTWLWKALHLDFGISFASQRPVLDDMLNFLPATL
                     ELAGAALVLILLTSVPLGIWAARHRDRLPDFAVRFIAFLGVSMPNFWLAFLLVMAFSV
                     YLQWLPAMGYGGWQHIILPAVSIAFMSLAINARLLRASMLDVAGQRHVTWARLRGLND
                     KQTERRHILRNASLPMITAVGMHIGELIGGTMIIENIFAWPGVGRYAVSAIFNRDYPV
                     IQCFTLMMVVVFVVCNLIVDLLNAALDPRIRRHEGAHA"
     gene            complement(4169..5743)
                     /gene="nikA"
                     /locus_tag="ACHOSC_RS09015"
                     /old_locus_tag="pgaptmp_001803"
     CDS             complement(4169..5743)
                     /gene="nikA"
                     /locus_tag="ACHOSC_RS09015"
                     /old_locus_tag="pgaptmp_001803"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009897 - external side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015413 - ABC-type nickel transporter
                     activity [Evidence IEA];
                     GO_function: GO:0016151 - nickel cation binding [Evidence
                     IEA];
                     GO_process: GO:0015675 - nickel cation transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nickel ABC transporter substrate-binding
                     protein"
                     /protein_id="HGU4547760.1"
                     /translation="MLSTLRRTLFALLACASFIVHAAAPDEITTAWPVNVGPLNPHLY
                     TPNQMFAQSMVYEPLVKYQADGSVIPWLAKSWTHSEDGKTWTFTLRDDVKFSNGEPFD
                     AEAAAENFRAVLDNRQRHAWLELANQIVDVKALSKTELQITLKSAYYPFLQELALPRP
                     FRFIAPSQFKNHETMNGIKAPIGTGPWILQESKLNQYDVFVRNENYWGEKPAIKKITF
                     NVIPDPTTRAVAFETGDIDLLYGNEGLLPLDTFARFSQNPAYHTQLSQPIETVMLALN
                     TAKAPTNELAVREALNYAVNKKSLIDNALYGTQQVADTLFAPSVPYANLGLKPRQYDP
                     QKAKALLEKAGWTLPAGKDIREKNGQPLRIELSFIGTDALSKSMAEIIQADMRQIGAD
                     VSLIGEEESSIYARQRDGRFGMIFHRTWGAPYDPHAFLSSMRVPSHADFQAQQGLADK
                     PLIDKEIGEVLATHDETQRQALYRDILTRLHDEAVYLPISYISMMVVSKPELGNIPYA
                     PIATEIPFEQIKPVKP"
     gene            complement(5854..6441)
                     /gene="acpT"
                     /locus_tag="ACHOSC_RS09020"
                     /old_locus_tag="pgaptmp_001804"
     CDS             complement(5854..6441)
                     /gene="acpT"
                     /locus_tag="ACHOSC_RS09020"
                     /old_locus_tag="pgaptmp_001804"
                     /EC_number="2.7.8.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4'-phosphopantetheinyl transferase AcpT"
                     /protein_id="HGU4547761.1"
                     /translation="MYRIVLGKVSTLSAAPLPPGLREQAPQGPRRERWLAGRALLSHT
                     LSPLPEIIYGEQGKPAFAPEMPLWFNLSHSGDDIALLLSDEGEVGCDIEVIRPRANWH
                     WLANAVFSLGEHAEMDAVHPDQQLEMFWRIWTRKEAIVKQRGGSAWQIVSVDSTYHSS
                     LSVSHCQLENLSLAICTPTPFTLTADSVQWIDSVN"
     gene            complement(6496..7545)
                     /locus_tag="ACHOSC_RS09025"
                     /old_locus_tag="pgaptmp_001805"
     CDS             complement(6496..7545)
                     /locus_tag="ACHOSC_RS09025"
                     /old_locus_tag="pgaptmp_001805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312350.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HGU4547762.1"
                     /translation="METPQPDKTGMHILLKLASLVVILAGIHAAADIIVQLLLALFFA
                     IVLNPLVTWFIRRGVQRPIAITIVVVVMLIALTALVGVLAASFNEFISMLPKFNKELT
                     RKLFKLQEMLPFLNLHMSPERMLQRMDSEKVVTFTTALMTGLSGAMASVLLLVMTVVF
                     MLFEVRHVPYKMRFALNNPQIHIAGLHRALKGVSHYLALKTLLSLWTGVIVWLGLELM
                     GVQFALMWAVLAFLLNYVPNIGAVISAVPPMIQVLLFNGVYECILVGALFLVVHMVIG
                     NILEPRMMGHRLGMSTMVVFLSLLIWGWLLGPVGMLLSVPLTSVCKIWMETTKGGSKL
                     AILLGPGRPKSRLPG"
     gene            7677..8894
                     /locus_tag="ACHOSC_RS09030"
                     /old_locus_tag="pgaptmp_001806"
     CDS             7677..8894
                     /locus_tag="ACHOSC_RS09030"
                     /old_locus_tag="pgaptmp_001806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417930.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HGU4547763.1"
                     /translation="MPEPVAEPALNGLRLNLRIVSIVMFNFASYLTIGLPLAVLPGYV
                     HDVMGFSAFWAGLVISLQYFATLLSRPHAGRYADLLGPKKIVVFGLCGCFLSGLGYLT
                     AGLTASLPVISLLLLCLGRVILGIGQSFAGTGSTLWGVGVVGSLHIGRVISWNGIVTY
                     GAMAMGAPLGVVFYHWGGLQALALIIMGVALVAILLAIPRPTVKASKGKPLPFRAVLG
                     RVWLYGMALALASAGFGVIATFITLFYDAKGWDGAAFALTLFSCAFVGTRLLFPNGIN
                     RIGGLNVAMICFSVEIIGLLLVGVATMPWMAKIGVLLAGAGFSLVFPALGVVAVKAVP
                     QQNQGAALATYTVFMDLSLGVTGPLAGLVMSWAGVPVIYLAAAGLVTIALLLTWRLKK
                     RPPEHVPEAASSS"
     gene            complement(8898..9455)
                     /gene="dcrB"
                     /locus_tag="ACHOSC_RS09035"
                     /old_locus_tag="pgaptmp_001807"
     CDS             complement(8898..9455)
                     /gene="dcrB"
                     /locus_tag="ACHOSC_RS09035"
                     /old_locus_tag="pgaptmp_001807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417929.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage sensitivity protein DcrB"
                     /protein_id="HGU4547764.1"
                     /translation="MRNLVKYVGIGLLVMGLAACDDKDTNATAQGSVAESNATGNPVN
                     LLDGKLSFSLPADMTDQSGKLGTQANNMHVWSDATGQKAVIVIMGDDPKEDLAVLAKR
                     LEDQQRSRDPQLQVVTNKAIELKGHKMQQLDSIISAKGQTAYSSVILGNVGNQLLTMQ
                     ITLPADDQQKAQTTAENIINTLVIQ"
     gene            complement(9528..10193)
                     /locus_tag="ACHOSC_RS09040"
                     /old_locus_tag="pgaptmp_001808"
     CDS             complement(9528..10193)
                     /locus_tag="ACHOSC_RS09040"
                     /old_locus_tag="pgaptmp_001808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:1990397 - queuosine salvage [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="7-cyano-7-deazaguanine/7-aminomethyl-7-
                     deazaguanine transporter"
                     /protein_id="HGU4547765.1"
                     /translation="MNVFSQTQRYKALFWLSLFHLLVITSSNYLVQLPVSIFGFHTTW
                     GAFSFPFIFLATDLTVRIFGAPLARRIIFAVMIPALLISYVISSLFYMGSWQGFGALA
                     HFNLFVARIATASFMAYALGQILDVHVFNRLRQSRRWWLAPTASTLFGNVSDTLAFFF
                     IAFWRSPDAFMAEHWMEIALVDYCFKVLISIVFFLPMYGVLLNMLLKRLADKSEINAL
                     QAS"
     gene            10414..10659
                     /gene="tusA"
                     /locus_tag="ACHOSC_RS09045"
                     /old_locus_tag="pgaptmp_001809"
     CDS             10414..10659
                     /gene="tusA"
                     /locus_tag="ACHOSC_RS09045"
                     /old_locus_tag="pgaptmp_001809"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0097163 - sulfur carrier activity
                     [Evidence IEA];
                     GO_process: GO:0002143 - tRNA wobble position uridine
                     thiolation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase TusA"
                     /protein_id="HGU4547766.1"
                     /translation="MTDLFSSPDHTLDALGLRCPEPVMMVRKTVRNMQPGETLLIIAD
                     DPATTRDIPGFCTFMEHELVAKETDGLPYRYLIRKGG"
     gene            complement(10761..12959)
                     /gene="zntA"
                     /locus_tag="ACHOSC_RS09050"
                     /old_locus_tag="pgaptmp_001810"
     CDS             complement(10761..12959)
                     /gene="zntA"
                     /locus_tag="ACHOSC_RS09050"
                     /old_locus_tag="pgaptmp_001810"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0019829 - ATPase-coupled monoatomic cation
                     transmembrane transporter activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Zn(II)/Cd(II)/Pb(II) translocating P-type ATPase
                     ZntA"
                     /protein_id="HGU4547767.1"
                     /translation="MSTPDNHGKKAPQFAAFKPLTTVQNANDCCCDGACSSTPTLSEN
                     VSGTRYSWKVSGMDCAACARKVENAVRQLAGVNQVQVLFATEKLVVDADNDIRAQVES
                     AVQKAGYSLRDEQSADEPQASRLKENLPLITLIVMMAISWGLEQFNHPFGQLAFIATT
                     LVGLYPIARQALRLIKSGSYFAIETLMSVAAIGALFIGATAEAAMVLLLFLIGERLEG
                     WAASRARQGVSALMALKPETATRLRNGEREEVAINSLRPGDVIEVAAGGRLPADGKLL
                     SPFASFDESALTGESIPVERATGDKVPAGATSVDRLVTLEVLSEPGASAIDRILKLIE
                     EAEERRAPIERFIDRFSRIYTPAIMAVALLVTLVPPLLFAASWQEWIYKGLTLLLIGC
                     PCALVISTPAAITSGLAAAARRGALIKGGAALEQLGRVTQVAFDKTGTLTVGKPRVTA
                     IHPATGISESELLTLAAAVEQGATHPLAQAIVREAQVAELAIPTAESQRALVGSGIEA
                     QVNGERVLICAAGKHPADAFAGLINELESAGQTVVLVVRNDDVLGIIALQDTLRADAA
                     TAISELNALGVKGVILTGDNPRAAAAIAGELGLEFKAGLLPEEKVKAVTKLNQHAPLA
                     MVGDGINDAPAMKAAAIGIAMGSGTDVALETADAALTHNHLRGLVQMIELARATHANI
                     RQNITIALGLKGIFLVTTLLGMTGLWLAVLADTGATVLVTANALRLLRRR"
     gene            complement(13033..13659)
                     /locus_tag="ACHOSC_RS09055"
                     /old_locus_tag="pgaptmp_001811"
     CDS             complement(13033..13659)
                     /locus_tag="ACHOSC_RS09055"
                     /old_locus_tag="pgaptmp_001811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysoplasmalogenase"
                     /protein_id="HGU4547768.1"
                     /translation="MLWSFIAVCLSAWLSVDASYRGPTWQRWVFKPLTLLLLLLLAWQ
                     APMFDAISYLVLAGLCASLLGDALTLLPRQRLMYAIGAFFLSHLLYTIYFASQMTLSF
                     FWPLPLVLLVLGALLLAIIWTRLEEYRWPICTFIGMTLVMVWLAGELWFFRPTAPALS
                     AFVGASLLFISNFVWLGSHYRRRFRADNAIAAACYFAGHFLIVRSLYL"
     gene            13800..14159
                     /locus_tag="ACHOSC_RS09060"
                     /old_locus_tag="pgaptmp_001812"
     CDS             13800..14159
                     /locus_tag="ACHOSC_RS09060"
                     /old_locus_tag="pgaptmp_001812"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2500 domain-containing protein"
                     /protein_id="HGU4547769.1"
                     /translation="MSKPPLFFIVIIGLIIVAASFRFMQQRREKADNDMAPLQQKLVL
                     VSNKREKPINDRRSRQQEVTPAGTSMRYEASFKPQSGGMEQTFRLDAQQYHALTVGDK
                     GTLSYKGTRFVSFVGEQ"
     gene            complement(14162..14431)
                     /locus_tag="ACHOSC_RS09065"
                     /old_locus_tag="pgaptmp_001813"
     CDS             complement(14162..14431)
                     /locus_tag="ACHOSC_RS09065"
                     /old_locus_tag="pgaptmp_001813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1145 family protein"
                     /protein_id="HGU4547770.1"
                     /translation="MLINIGRLLMLCVWGFLILNLVHPFPRPLNIFVNVALIFTVLMH
                     GMQLALLKSTLPKDGPQMTTAEKVRIFLFGVFELLVWQKKFKVKK"
     gene            complement(14421..15017)
                     /gene="rsmD"
                     /locus_tag="ACHOSC_RS09070"
                     /old_locus_tag="pgaptmp_001814"
     CDS             complement(14421..15017)
                     /gene="rsmD"
                     /locus_tag="ACHOSC_RS09070"
                     /old_locus_tag="pgaptmp_001814"
                     /EC_number="2.1.1.171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(966)-N(2))-methyltransferase"
                     /protein_id="HGU4547771.1"
                     /translation="MKKPNHSGSGQIRIIGGQWRGRKLPVPDSPGLRPTTDRVRETLF
                     NWLAPVIVDAQCLDCFAGSGALGLEALSRYAAGATLIEMDRAVSQQLIKNLATLKAGN
                     ARVVNSNAMSFLAQKGTPHNIVFVDPPFRRGLLEETINLLEDNGWLADEALIYVESEV
                     ENGLPTVPANWSLHREKVAGQVAYRLYQREAQGESDAD"
     gene            15167..16663
                     /gene="ftsY"
                     /locus_tag="ACHOSC_RS09075"
                     /old_locus_tag="pgaptmp_001815"
     CDS             15167..16663
                     /gene="ftsY"
                     /locus_tag="ACHOSC_RS09075"
                     /old_locus_tag="pgaptmp_001815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005047 - signal recognition particle
                     binding [Evidence IEA];
                     GO_process: GO:0006614 - SRP-dependent cotranslational
                     protein targeting to membrane [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle-docking protein
                     FtsY"
                     /protein_id="HGU4547772.1"
                     /translation="MAKEKKRGFFSWLGFGQKEQTPEKETEVQNEQPVVEEIVQAQEP
                     VKASEQAVEEQPQAHTEAEAETFAADVVEVTEQVAESEKAQPEAEVVAQPEPVAEETP
                     EPVAIEREELPLPEDVNAEEVSPEEWQAEAETVEIVEAAEEEAAKEEITDEELEAQAL
                     AAEAAEEAVMVVPPAEEEQPVEEIAQEQEKPTKEGFFARLKRSLLKTKENLGSGFISL
                     FRGKKIDDDLFEELEEQLLIADVGVETTRKIITNLTEGASRKQLRDAEALYGLLKEEM
                     GEILAKVDEPLNVEGKTPFVILMVGVNGVGKTTTIGKLARQFEQQGKSVMLAAGDTFR
                     AAAVEQLQVWGQRNNIPVIAQHTGADSASVIFDAIQAAKARNIDVLIADTAGRLQNKS
                     HLMEELKKIVRVMKKLDVEAPHEVMLTIDASTGQNAVSQAKLFHEAVGLTGITLTKLD
                     GTAKGGVIFSVADQFGIPIRYIGVGERIEDLRPFKADDFIEALFARED"
     gene            16666..17334
                     /gene="ftsE"
                     /locus_tag="ACHOSC_RS09080"
                     /old_locus_tag="pgaptmp_001816"
     CDS             16666..17334
                     /gene="ftsE"
                     /locus_tag="ACHOSC_RS09080"
                     /old_locus_tag="pgaptmp_001816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division ATP-binding protein FtsE"
                     /protein_id="HGU4547773.1"
                     /translation="MIRFEHVSKAYLGGRQALQGVTFHMQPGEMAFLTGHSGAGKSTL
                     LKLICGIERPSAGKIWFSGHDITRLKNREVPFLRRQIGMIFQDHHLLMDRTVYDNVAI
                     PLIIAGASGDDIRRRVSAALDKVGLLDKAKNFPIQLSGGEQQRVGIARAVVNKPAVLL
                     ADEPTGNLDDALSEGILRLFEEFNRVGVTVLMATHDINLISRRSYRMLTLSDGHLHGG
                     VGHE"
     gene            17327..18385
                     /gene="ftsX"
                     /locus_tag="ACHOSC_RS09085"
                     /old_locus_tag="pgaptmp_001817"
     CDS             17327..18385
                     /gene="ftsX"
                     /locus_tag="ACHOSC_RS09085"
                     /old_locus_tag="pgaptmp_001817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="permease-like cell division protein FtsX"
                     /protein_id="HGU4547774.1"
                     /translation="MNKRDAINHIRQFGGRLDRFRKSVGGSGDGGRNAPKRAKSSPKP
                     VNRKTNVFNEQVRYAFHGALQDLKSKPFATFLTVMVIAISLTLPSVCYMVYKNVNQAA
                     TQYYPSPQITVYLQKTLDDDAAAGVVAQLQAEQGVEKVNYLSREDALGEFRNWSGFGG
                     ALDMLEENPLPAVAVVIPKLDFQGTESLNTLRDRITQINGIDEVRMDDSWFARLAALT
                     GLVGRVSAMIGVLMVAAVFLVIGNSVRLSIFARRDSINVQKLIGATDGFILRPFLYGG
                     ALLGFSGALLSLILSEILMLRLSSAVAEVAQVFGTKFDINGLSFDECLLLLLVCSMIG
                     WVAAWLATVQHLRHFTPE"
     gene            18630..19484
                     /gene="rpoH"
                     /locus_tag="ACHOSC_RS09090"
                     /old_locus_tag="pgaptmp_001818"
     CDS             18630..19484
                     /gene="rpoH"
                     /locus_tag="ACHOSC_RS09090"
                     /old_locus_tag="pgaptmp_001818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoH"
                     /protein_id="HGU4547775.1"
                     /translation="MTDKMQSLALAPVGNLDSYIRAANAWPMLSADEERALAEKLHYH
                     GDLEAAKTLILSHLRFVVHIARNYAGYGLPQADLIQEGNIGLMKAVRRFNPEVGVRLV
                     SFAVHWIKAEIHEYVLRNWRIVKVATTKAQRKLFFNLRKTKQRLGWFNQDEVEMVARE
                     LGVTSKDVREMESRMAAQDMTFDLSSDDDSDSQPMAPVLYLQDKSSNFADGIEDDNWE
                     EQAANRLTDAMQGLDERSQDIIRARWLDEDNKSTLQELADRYGVSAERVRQLEKNAMK
                     KLRAAIEA"
     gene            19755..20858
                     /gene="livJ"
                     /locus_tag="ACHOSC_RS09095"
                     /old_locus_tag="pgaptmp_001819"
     CDS             19755..20858
                     /gene="livJ"
                     /locus_tag="ACHOSC_RS09095"
                     /old_locus_tag="pgaptmp_001819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched chain amino acid ABC transporter
                     substrate-binding protein LivJ"
                     /protein_id="HGU4547776.1"
                     /translation="MNIKGKALLAGCIALAFSNMALAEDIKVAVVGAMSGPVAQYGDQ
                     EFTGAEQAVADINAKGGIKGNKLQIVKYDDACDPKQAVAVANKVVNDGIKYVIGHLCS
                     SSTQPASDIYEDEGILMITPAATAPELTARGYQLILRTTGLDSDQGPTAAKYILEKVK
                     PQRIAIVHDKQQYGEGLARAVQDGLKKGNANVVFFDGITAGEKDFSTLVARLKKENID
                     FVYYGGYHPEMGQILRQARAAGLKTQFMGPEGVANVSLSNIAGESAEGLLVTKPKNYD
                     QVPANKPIVDAIKAKKQDPSGAFVWTTYAALQSLQAGLNQSDDPAEIAKYLKANSVDT
                     VMGPLTWDEKGDLKGFEFGVFDWHANGTATDAK"
     gene            complement(21057..21440)
                     /gene="panM"
                     /locus_tag="ACHOSC_RS09100"
                     /old_locus_tag="pgaptmp_001820"
     CDS             complement(21057..21440)
                     /gene="panM"
                     /locus_tag="ACHOSC_RS09100"
                     /old_locus_tag="pgaptmp_001820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0031638 - zymogen activation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate 1-decarboxylase autocleavage activator
                     PanM"
                     /protein_id="HGU4547777.1"
                     /translation="MKLTIIRLENFSDQDRIDLQKIWPEYSPSSLQVDDNHRIYAARF
                     NERLLAAVRVTLSGTEGALDSLRVREVTRRRGVGQYLLEEVLRNNPGVSCWWMADAGL
                     EDRGVMTAFMQALGFTAQQGGWEKR"
BASE COUNT         5115 a         5677 c         5957 g         4771 t
ORIGIN      
        1 atgaacggca gcgggccggg cagggcgatg tcggtttcac cgggaaggac ctttgcaccc
       61 agcagcggat tgaccggatg gccggacgtc accccgccgg ggcacaccga acaggccacg
      121 ccggtggggg caccaatacg taccccggct gaaccctgaa caatgctacc gccatactgc
      181 gtcatgtcac cctgacgcgc cgccggtttt ccgctcatcg taaaatcctt ttctattatt
      241 ttaatgacag tatcttcata cgaaaaagcg aagaaaaact tgtggtcaca atgaattgaa
      301 gatttttagg aatatattta gaacgttaca aaagagaagg ttcggatagg gtatttagag
      361 aggaatcagg tgtgctagtg gccctttgtt gatcgttcac caaaggaccc aataatagac
      421 tcaatcttcc ttcggcaagc actgcaaatg cccgtgccgc acgccgcgct gggcgataac
      481 gtcatcggca aaatgctgca cgtcgcccat atcacctttc agcacggcga tttccagaca
      541 gtcgtcgtga ttgatatgca catgcagcgt ggcgacggag agatcgtggt gatggtgctg
      601 ggtggagaca atgcggctgg ctaagtcgcg tttttcgtgt tcatacacat aggagagcac
      661 cgcgaaacct tgcgtgccgt gctgctgagt ggcctcctgg gccagagcgc tacgcagaat
      721 atcgcggata gcttcggaac gattgttata accacgacgc tggctcaggc tgtccagcgt
      781 ctccagtaaa tcgtcatcaa gcgtgatggt gactcgttgc atttgcgtta aaccttttct
      841 gtggtgcgac ggcgcacggg gaatgcgggt aataccgcgt tttgtagcac acgtccggcg
      901 tcagaggaaa aggttaattt atctcccacc acctgggttt cgacgatttg tccgttgtcc
      961 atcaccatca cccgctgaca gaagcgttcc accaggcgta agtcgtgggt gatgaacagg
     1021 caggcggtgc caaactgttg ttgtagcttt ttcagcaggc gaatgacgcc cgcctgtaac
     1081 acgagatcaa ggttagaaac ggcttcatcc agaatcagta gtttcggttc gaccgccagc
     1141 gcgcgagcca ggcagacgcg ctggagctgg ccgccgctta actgcggtgg gcgtttgtcg
     1201 agaacgctgt catcgagatc gaccgccttc agcatttcgc tggcgcgcgc cagttgttcg
     1261 gattttttca gtgatagcag gtggcgcatc ggttcacgca ggatctcgcg cacggttttg
     1321 cgcggattca cggcgctgat ggagtcctga aataccatct gaatatcgcg gcggaacgct
     1381 ttacgctggg cgcgattgag tttcgccagc ggttcgccac gccagctaat attcccctgc
     1441 gcgggcgatt ctaaacccac cagcaaccgc gcgagggtac ttttcccgca gccgctgcgc
     1501 cccagcaggg cgacggtttc gccgcttttc agggtcaggg aaacgttatt cagcaccgcc
     1561 tgatgttgat gttttccgtt aaatccaccg tgcgcatagt gatgggaaag gccggagatg
     1621 ttaagtaaag tcatgatgcc agctccatac cgtagagggc gagatgagcg gaaaccaggc
     1681 tgcgcgtcac tgtatgtttg ggggcgttaa acagcgtttc tacatcgccc tgttcgacaa
     1741 tcttaccgtc agacatcacc gccacgtcat ccgccagacg cgccacaacg cccatatcat
     1801 gggtcaccag cagcattccc ggcgcttgtt tttgcataat gctttccagc agatcgagga
     1861 tgcgcgcctg tgctaccacg tcgaggtcgg tggtcggttc atcggcgatg ataaacggtg
     1921 attcacacag caccgccatc gcaatcatca tgcgctgcaa catgccgccg ctcatctcga
     1981 acgggtacag cttcagcacg cgcgcggcgt tttccagccc caccgcttct atggcagcgg
     2041 taagcgtagc gtcatcggcg ggtttcccta acgccaggca ggtttcccgc gcgtgggtgt
     2101 gcatggtgtg cagtggatta aaggcgctgc gcgggttctg catgatggtg gcaattttga
     2161 tgccgcgcag ggcgcaaggc gaaaccggtt tgccatcggc taaaatttcc cccgccgtct
     2221 ggcgaacgcc agcgggcaaa atgcccagcg tcgcggcgca ggttaatgat ttcccgctgc
     2281 cgctaccgcc gactaacgcc agcacgcgcc cgcgttgcag ggttaacgat acaccgtgta
     2341 ccagcggctg cgcggcctgt agcgcgatat tacgtagttc aatctgttgc ggcattagtg
     2401 tgcgtgctcc gtcaccagat gagggtccag atgatcgcgc agtgcgtcac ccaccaggtt
     2461 aaaggccatc acgctgataa acagcgccag ccccggccag aacatttgca gcggctgggt
     2521 ccagatatac tggcgcgcgt cgttaatcat cacgccccat tcggcggtcg gcgcggtcac
     2581 accgaggcca aggaaagaca tccccgcgac gtgcagcatc atatggccga tatccagcgt
     2641 tgccagcacc agcagcgaag ggatcaccgc gcctgccaga tgatcgacaa acacccgcac
     2701 atggcccgca ccggaaagcc gtgacgccag cacaaactcg cgttggcgta gtgaaatcac
     2761 caggctgcgc accatgcgtg cataccacgc ccagtgcgac agggcgatgg cgataattac
     2821 gttggtcagc ccggtgccga gcacgccaac cataaagaac gacagaatcg aggtcgggaa
     2881 ggtcataaac atatcggcga cgcgcatggt ggcctgatca acgcgcccgc caatcaaccc
     2941 ggcgctgccg ccaataacca gccctaatgt cagcaccagc agcaggcagg ccattaccga
     3001 accgagcgac acgcgggtcg ctgtcatcag ccgcgagaaa atatcgcgac ctaagtgatc
     3061 ggtgcccagc cagtgctgcg catccggcga aagcaggcgc gacggcaaat caatcgcctg
     3121 ggggtcatac ggcagccacc actggctggt gagcgcaatc agcgccagca gggcgataat
     3181 gatcagcgcc aggcgtaccg accagcggga agagaggaaa aagttcacgc gtgcgctcct
     3241 tcatgacgac gaatgcgcgg gtccagcgcg gcgttgagca aatcgacaat caaattacag
     3301 accacaaaaa ccaccaccat catcagcgta aagcactgga tcaccggata gtcacggtta
     3361 aaaatcgccg acaccgcata gcgcccgacg cccggccagg caaagatgtt ttcgataatc
     3421 atcgtcccgc caatcagttc gccgatatgc atccccacgg cggtgatcat cggcagcgag
     3481 gcattgcgca ggatgtggcg acgttcggtc tgtttgtcgt tcaggccgcg cagacgcgcc
     3541 caggtgacgt gacgctgacc ggcgacgtcc agcatactgg cgcgcagtaa acgcgcgtta
     3601 atcgccagcg acataaaggc aatggaaacc gcaggcaaaa tgatgtgctg ccagccgccg
     3661 taacccatcg cgggtagcca ttgcagatac accgaaaacg ccatcaccag taaaaacgcc
     3721 agccagaagt taggcatcga cacgccaaga aacgcgatga aacgtacggc gaaatccggc
     3781 agacggtcgc gatggcgcgc cgcccagata ccgagcggta cggaagtgag cagaattaat
     3841 accagcgccg cacctgcaag ttccagcgtg gcgggcagga agttcagcat atcgtccagt
     3901 accgggcgtt ggctggcgaa tgagatacca aagtcaagat gcagcgcctt ccacaaccag
     3961 gtaccgtact ggacgtacag cggctgatcc agcccgagca tggtgcgggt agaggccagc
     4021 atctccggcg tcggcggcag gttagataaa cgcagataat cgagcgccgg gtcgccggta
     4081 ccgaggcgca gcatcagaaa aatgatcacc gaggcggcga gcaccatcgg gatcagcagc
     4141 agaaagcggc gtaatacgta acgcaacatt aaggtttcac cggtttaatc tgttcgaacg
     4201 gaatttcggt ggcgatcggc gcgtagggga tgttacccag ctccggtttt gataccacca
     4261 tcattgagat gtaactgata ggcagataaa ccgcctcgtc atgcagacgg gtcagaatgt
     4321 cgcgatacag cgcctgacgt tgcgtttcgt catgggtcgc cagcacttcg ccgatctctt
     4381 tatcaatcag cggtttgtcg gctaatcctt gctgtgcctg gaagtcagcg tgtgacggta
     4441 cgcgcattga actgaggaag gcgtgtggat catatggcgc gccccaggtg cggtggaaaa
     4501 tcatgccaaa acgaccgtcg cgctgacgag catagatact gctctcttct tcgccaatca
     4561 gcgagacatc tgcgccaatc tggcgcatat cagcctgaat gatttccgcc atcgatttgc
     4621 ttaacgcatc ggtgccgatg aacgacagtt caatgcgcag cggctgacca tttttctcgc
     4681 ggatgtcttt gcccgcaggc agcgtccaac cggctttttc cagcaacgct ttcgctttct
     4741 gcggatcgta ctggcgcggt ttcaggccga ggttggcgta gggcacagaa ggggcaaaca
     4801 gggtgtcggc gacctgctgg gtgccataca acgcgttatc aatcagcgat tttttgttta
     4861 ccgcgtaatt aagagcttca cgtactgcca gctcgttggt gggggctttg gcggtattga
     4921 gcgccagcat cacggtttcg atcggctgtg acagttgggt gtggtaagcc ggattctggc
     4981 taaagcgggc gaaggtatcg agcggtaata acccttcgtt tccgtacagc aggtcgatat
     5041 cgccagtttc aaacgccacc gcgcgggtag tcgggtccgg gatgacgtta aaggtgatct
     5101 ttttaatcgc tggcttttcg ccccagtagt tttcgttacg gacgaagaca tcgtactgat
     5161 tcagtttcga ttcctgcaaa atccacggtc cggtgccaat cggcgcttta attccgttca
     5221 tggtttcatg gtttttaaac tgcgagggag cgataaaacg gaaaggacgg ggcagggcca
     5281 gttcttgcag gaaaggatag taggcgcttt tcagggtaat ttgcagctct gttttactga
     5341 gtgctttaac atcaacaatc tggtttgcca gctccagcca ggcgtgacgt tgacggttat
     5401 cgagcactgc gcggaagttt tctgccgccg cctcggcatc gaacggttca ccgttggaga
     5461 atttcacgtc atcacgcagg gtgaaggtcc aggttttacc atcttctgaa tgagtccagc
     5521 tttttgccag ccacgggatc accgaaccgt ctgcctgata tttcaccaat ggttcataaa
     5581 ccatgctctg ggcgaacatc tggttaggcg tgtaaaggtg cgggtttagt ggcccgacat
     5641 tcaccggcca ggcggtggtg atttcatctg gtgcagcggc atggacgata aaagacgcac
     5701 aagccagcag cgcaaataga gtgcggcgga gtgtggagag catggtaacc ccaatggatt
     5761 aaaatagatg gcggaaataa gtatgacgat tttaagtatt cgtcatactg attacctgtt
     5821 ctggatcaat agatgggcag tcgggtgggc ggatcagtta actgaatcga tccattgcac
     5881 actgtcggcg gtgagcgtaa agggagtagg ggtgcagatc gccaggctta aattttcaag
     5941 ctgacaatgg ctgactgaca gcgaggagtg ataggtgctg tctacgctga cgatttgcca
     6001 ggcgctgccg ccacgctgtt taacgatggc ttctttgcgc gtccagatgc gccagaacat
     6061 ttccagttgc tgatcaggat gcacggcgtc catctcagcg tgttccccga ggctgaatac
     6121 ggcgttcgcc agccagtgcc agttggcgcg cgggcgaatc acttcgatat cgcagccgac
     6181 ttcgccttca tcactcaaca gcagggcgat atcgtcaccg ctatggctta agttgaacca
     6241 tagcggcatt tccggcgcaa atgcaggttt gccttgttcg ccatagatga tctccggtag
     6301 cggggaaagc gtgtgcgaaa gcaatgcacg ccccgccagc cagcgttcgc gtcgtggacc
     6361 ttgcggtgct tgctcgcgta aacccggtgg cagtggagct gcgcttaagg tcgaaacttt
     6421 ccccagaact atccgataca tatcagggcc aacgtttaat ggaaaatgaa agtgcgtatc
     6481 gtatcacttg tcgcctcatc ccggtaaccg acttttcggt ctgcctggcc ccagtaaaat
     6541 cgccagtttg ctaccgcctt tggtggtttc catccagatt ttacacacac tggttaatgg
     6601 caccgaaagt agcatcccta ccgggccgag cagccatccc caaattaaca atgaaagaaa
     6661 taccaccatg gtggacatcc ccaggcgatg gcccatcatc cgtggttcta aaatattgcc
     6721 gatgaccata tggaccacta aaaacaatgc gccgaccaga atacattcgt aaacaccatt
     6781 aaacagcagc acctgaatca ttggcggtac ggcggaaatt accgcgccga tattgggcac
     6841 gtagttgagc aaaaacgcca gtactgccca catcagcgca aactgcaccc ccatcagctc
     6901 cagccccagc cagacgatga cacctgtcca taaactgagt agcgtcttca atgcaagata
     6961 gtgcgaaacg cctttaagtg cgcggtgtaa tcctgcgatg tgaatctgtg ggttattcag
     7021 cgcaaaacgc attttgtaag ggacgtggcg cacttcaaac agcataaaaa ctacggtcat
     7081 caccagcaaa agcacgctcg ccattgcccc ggaaagcccg gtcattagcg ctgtggtgaa
     7141 ggtaaccact ttttccgagt ccatccgctg cagcattcgc tccggcgaca tatgcaaatt
     7201 aagaaaaggc aacatctcct gcaatttaaa aagtttgcgc gtcaactcct tattaaactt
     7261 cggcagcata gagataaatt cgttaaacga tgccgccagt acgccgacca gcgcagttag
     7321 tgcgatcagc atcaccacca ccacaatcgt aatggcaatg gggcgttgta ctccccgacg
     7381 aataaaccag gtgacgagcg ggttgaggac gatggcaaaa aacagcgcca gtaacagctg
     7441 cacaatgata tctgccgctg cgtgaatgcc cgcgaggatc actaccagcg aggccagctt
     7501 gagcagaatg tgcatgcccg ttttatcggg ttgaggggtt tccattgggg cttccttgtg
     7561 actttttgta ttaagtgtag cgggagtcac gccagcaata ttctgattct tactgctgat
     7621 tttcacgtca gcacgtggta aaaatgaaac actgttgtaa aaatgtggtg atcctcatgc
     7681 ccgaacccgt agccgaaccc gcgctaaacg gattgcgcct gaatttgcgc attgtctcca
     7741 ttgtcatgtt taacttcgcc agctacctca ccatcgggtt gccgctcgct gtattaccgg
     7801 gctatgtcca tgatgtgatg ggctttagcg ctttctgggc aggattggtt atcagcctgc
     7861 aatatttcgc caccttgctg agccgtcctc atgccggacg ttacgccgat ttgctgggcc
     7921 ccaaaaagat tgtcgtcttc ggtttatgcg gctgcttttt gagcggtctg ggatatctga
     7981 cggcaggatt aaccgccagt ctgcccgtca tcagcctgtt attactttgc ctgggacgcg
     8041 tgatccttgg gattgggcaa agttttgccg gaacgggatc gaccctgtgg ggcgttggcg
     8101 tggttggctc gctgcatatc gggcgagtga tttcgtggaa cggcattgtc acttacgggg
     8161 cgatggcgat gggtgcgccg ttaggcgtcg tgttttatca ctggggcggc ttgcaggcgt
     8221 tagcgttaat cattatgggc gtggcgctgg tggccatttt gttggcgatc ccgcgtccga
     8281 cggtaaaagc cagtaaaggc aaaccgctgc cgtttcgcgc ggtgcttggg cgcgtctggc
     8341 tgtacggtat ggcgctggca ctggcttccg ccggatttgg cgtcatcgcc acctttatca
     8401 cgctgtttta tgacgctaaa ggttgggacg gtgcggcttt cgcgctgacg ctgtttagct
     8461 gtgcgtttgt cggtacgcgt ttgttattcc ctaacggcat taaccgtatc ggtggcttaa
     8521 acgtagcgat gatttgcttt agcgttgaga taatcggcct gctactggtt ggcgtggcga
     8581 ctatgccgtg gatggcgaaa atcggcgtct tactggcggg ggccgggttt tcgctggtgt
     8641 tcccggcatt gggtgtagtg gcggtaaaag cggttccgca gcaaaatcag ggggcggcgc
     8701 tggcaactta caccgtattt atggatttat cgcttggcgt gactggacca ctggctgggc
     8761 tggtgatgag ctgggcgggc gtaccggtga tttatctggc ggcggcggga ctggtcacaa
     8821 tcgcgttatt actgacgtgg cgattaaaaa aacggcctcc ggaacacgtc cctgaggccg
     8881 cctcatcatc ttaaaactta ctgaataacc agcgtattaa tgatgttttc tgcggtggtc
     8941 tgcgcttttt gctgatcgtc agctggcaga gtaatttgca tggtcagcag ttgattaccc
     9001 acgttaccca gaataacgga agagtacgcc gtctggcctt tcgcggagat aatactgtct
     9061 aactgctgca ttttgtgacc tttcagctca atggctttat tggttaccac ttgcagctgc
     9121 ggatcgcggc tacgttgctg atcttccaga cgcttcgcca gcaccgccag atcttctttt
     9181 ggatcatcgc ccatgatgac gatgactgct ttctgcccgg tggcgtcgga ccagacatgc
     9241 atgttattgg cctgcgttcc cagcttaccg ctctggtcgg tcatatccgc tggcagcgag
     9301 aaacttaact tgccatcaag caggttgacg ggattcccgg tagcgttact ttccgcgacc
     9361 gaaccctgcg ccgtagcgtt agtgtcttta tcatcacagg ccgcaagccc cataaccagc
     9421 aggccaattc cgacatattt aaccagattg cgcattgact tcttcctttc gataaacggc
     9481 cataacggct cattcatcca tcttatcaca actctgataa cgaaccttta actcgcctgc
     9541 aaagcgttga tttcggattt atctgccagt cttttcaaca gcatattgag taatacgcca
     9601 tacattggca ggaagaaaac gatactgatt aacactttga aacagtaatc gaccagcgcg
     9661 atttccatcc agtgttcagc cataaaggca tccgggctac gccagaaggc aatgaagaaa
     9721 aaggccagcg tgtcgctgac gttaccgaac agtgtggacg ctgtcggtgc cagccaccag
     9781 cggcgacttt gacgcaggcg gttaaaaacg tgcacgtcga ggatttgccc cagcgcgtag
     9841 gccatgaaac tggcggtggc gatacgggcg acaaacaggt tgaagtgggc gagtgcgccg
     9901 aatccctgcc atgaacccat atagaacagc gacgagatga cataggagat taataacgca
     9961 gggatcatta ccgcgaagat aatgcgtcgg gccagcggtg cgccaaaaat acgcacggtc
    10021 aggtcggtag caagaaaaat aaacggaaag ctaaacgcgc cccaggtggt atggaaacca
    10081 aaaatggaga cgggaagctg aaccagatag ttactggagg tgatcaccag cagatgaaat
    10141 aacgataacc agaacaacgc cttatagcgt tgagtttgcg agaaaacgtt catattgtac
    10201 ctttttgatt aaccattggg gtgagggaac ccaatacgta cgacacgtct gttaccttaa
    10261 cataataacg tgcttaccct tttttcgagc cgccgcatga tactgcttta cgttgacaat
    10321 gcaatggtta attttcacgc aatcgttaac ctggtttgct tacggactca cagggcgtaa
    10381 actagcgccg tttttttatg tgatgagaag aaaatgaccg atctcttttc cagccctgac
    10441 cacacactcg acgcacttgg cctgcgctgc ccggaacccg taatgatggt gcgcaaaact
    10501 gtgcgcaata tgcagcccgg cgaaacgttg ctgattatcg ccgacgatcc ggccactacc
    10561 cgcgatattc ctgggttttg tacctttatg gaacacgaac tggttgcgaa agagacggat
    10621 ggactgcctt atcgttattt gattcgtaaa ggcggttgat gggggctgat tggcttcgat
    10681 gccgcctttt cccctcaccc taaccctctc cccagagggg cgaggggacc gatcgcgctc
    10741 aatgttgcga tcggtttgcc ttatctcctg cgcaacaatc ttaacgcatt cgctgtcacc
    10801 agcaccgtcg cacccgtatc tgccagcacc gccagccata gtccggtcat ccctaacagc
    10861 gtggtgacga ggaagatccc tttcagtccc agcgcaatgg tgatgttctg gcggatattg
    10921 gcgtgagtgg cgcgtgccag ttcaatcatt tgcaccaggc cgcgcaggtg gttatgggtt
    10981 aatgctgcat cggcggtttc cagcgccacg tctgtgccgc tgcccattgc aatcccaatg
    11041 gcggcagctt tcatcgctgg cgcgtcgtta ataccatcac cgaccatcgc cagcggcgca
    11101 tgttgattca gcttggtcac cgctttgact ttctcttccg gcaataggcc cgctttaaac
    11161 tccagcccca gctccccggc gattgccgcc gctgcgcgtg gattatcgcc ggtgaggatc
    11221 actcctttaa ctcccagcgc gttcagttca ctgatggcag ttgcagcgtc ggcgcgcagg
    11281 gtatcctgca atgcaatgat acccagcacg tcatcattac gtactaccag cacaaccgtt
    11341 tgcccggcgc tttccagttc attaatcaaa ccagcaaatg catcagcggg atgtttcccg
    11401 gcagcgcaaa tcaatacgcg ctcaccgtta acctgcgctt caatgccaga cccgaccagc
    11461 gcccgctgtg attcggcggt gggaatggcg agttcagcaa cctgtgcttc tcgcacgatg
    11521 gcctgcgcca gtggatgcgt cgcaccttgc tcgaccgccg ctgccagtgt cagcagttca
    11581 gattcactaa tacccgttgc cggatgaatc gcggtaacgc gcggtttacc gacggtcagc
    11641 gtaccggttt tatcaaacgc cacctgggta acacgaccta gttgttccag cgccgctccg
    11701 cctttaatta acgccccacg acgcgctgct gccgccagcc cggaggtaat cgccgcgggc
    11761 gtggagataa ctaacgcaca cgggcagcca atcagcagca gcgtcaggcc tttataaatc
    11821 cactcttgcc agctggcggc aaacagcagc ggtggcacca gcgtcaccag cagagcgacg
    11881 gccataatcg cgggcgtata gatacggctg aaacggtcga taaaccgctc aatgggtgcg
    11941 cgacgctctt cggcttcttc gatcagtttc agaatccggt caatggcgct ggcacccggt
    12001 tctgacagca cttccagcgt taccagacgg tctacgctgg tggctccagc agggacttta
    12061 tcgcccgttg cgcgctccac cggaatagat tcgccggtca gggcgctttc atcaaaactg
    12121 gcaaacggtg agagcagttt accgtcggca ggcaaacgtc cacctgcggc gacttcaatc
    12181 acatcgccag ggcgcaggct gttaatcgcc acctcttccc gctcaccgtt acgcaggcgc
    12241 gtggcggttt ctggtttcag cgccattaac gcgctgaccc cttgacgtgc gcggctggcg
    12301 gcccagcctt ccagtcgttc gccaatcaaa aacagcagca acaccatcgc agcttcagcc
    12361 gttgcgccaa taaaaagcgc accaatagcg gctacgctca ttaaggtttc aatggcgaag
    12421 tagctgccgg atttgatcaa ccgtaatgcc tgacgagcaa tcgggtacag cccaaccagt
    12481 gtggtcgcga taaacgccag ttgcccgaac ggatgattga actgctccag accccagctg
    12541 attgccatca tcacgattag cgtaatcagc ggcagattct ctttcaggcg tgatgcttgc
    12601 ggttcgtcgg cggactgttc atcgcgcagg gagtagcccg ctttttgcac cgcagattca
    12661 acctgggcac ggatgtcatt gtcggcatca accaccagtt tttcggtggc gaacaacacc
    12721 tgtacctgat tcacgcctgc aagctggcgc acggcatttt ctaccttgcg cgcacaggcg
    12781 gcgcagtcca tgccgctgac tttccagcta tagcgggtgc cggagacgtt ttcagagaga
    12841 gttggcgtgc tggaacatgc gccgtcgcag caacagtcgt tggcgttctg taccgtggtt
    12901 agcggtttga acgcagcaaa ttgaggggct ttcttgccgt gattgtcagg agtcgacatg
    12961 gcatcctccg gttaagtttt ttctcattaa ccgaaggata cactctggag tcgactccag
    13021 agtcaagttt tatcagagat acagcgagcg gacgatcagg aagtgaccgg caaagtagca
    13081 ggccgcagca atcgcgttat ccgcacggaa gcgtcggcga tagtgactac ccagccagac
    13141 aaagttactg ataaacagca acgaagcgcc gacaaacgca gagagcgccg gagcggtcgg
    13201 acggaagaac cacagttcac ctgccagcca caccatcacc agcgtcatgc cgataaacgt
    13261 gcagataggc caacgatact cttccagccg cgtccagata atcgccagta acagcgcgcc
    13321 cagaaccagc agcaccagtg gcagaggcca gaagaaagag agcgtcatct gactggcgaa
    13381 atagatggtg tacagcaggt gcgagaggaa aaacgcgccg atggcgtaca tcagacgttg
    13441 acgtggcaac agggttagcg catcgcccag cagtgaggcg cacagccccg ccagcaccag
    13501 atagctaatg gcgtcgaaca tcggcgcttg ccaggccagt aacagcagga gaagaagggt
    13561 taatggttta aatacccagc gttgccaggt tggcccacga tacgatgcat ccacagatag
    13621 ccatgcggaa agacagacag cgataaacga ccaaagcatc ttagttcctt gaattcgtta
    13681 ttttttcgta agcggaatgc ctacggtctt gcttccagtt tagtggcgag ggtggacgga
    13741 tgacaacacc ataactggca gggtatgctt aattccgcat tttccgatga gggataaaga
    13801 tgagcaaacc acctcttttc tttattgtta tcattggctt aatcatcgtc gccgcatcgt
    13861 ttcgttttat gcaacaacga cgggaaaaag ctgataatga tatggcacca ctccagcaaa
    13921 agctggtgtt ggtgagcaac aagcgggaaa aaccgattaa cgatcgccgt tcgcgccagc
    13981 aggaagtgac tccggcaggc accagtatgc gctatgaggc aagcttcaaa ccgcaaagcg
    14041 gaggaatgga gcagacgttt cgcctcgacg cccagcagta ccacgccctg acagtgggcg
    14101 ataaaggtac gctgagctat aaaggaacgc gctttgtcag ctttgtaggc gaacaataag
    14161 gttatttttt aactttaaat ttcttctgcc agaccagcag ttcaaacacg ccgaaaagga
    14221 aaatccgtac cttttcggcg gtggtcatct gcgggccatc tttcggtaaa gtggatttca
    14281 atagcgccag ctgcatacca tgcatcagca cggtaaaaat cagcgccacg ttaacgaaaa
    14341 tattcagcgg acgtgggaag ggatgcacca ggttgaggat taaaaatccc caaacacaga
    14401 gcattaacaa acgaccaata ttaatcagca tcactttctc cttgtgcttc gcgttgatac
    14461 agccgataag ccacctgacc cgccactttt tcccgatgta atgaccagtt tgccggaaca
    14521 gtgggcagac cgttttcgac ttcgctttcg acataaatca gggcttcgtc agccagccag
    14581 ccgttatctt ccagtaaatt tatcgtctct tctaacaagc cacggcggaa cggtggatcg
    14641 acaaacacga tattatgcgg tgtacctttt tgcgccagga atgacatcgc gttgctgttc
    14701 accacgcgtg cattgcctgc ttttagtgtc gccagattct taattaactg ctgagaaacc
    14761 gcgcgatcca tctcaatcaa cgttgccccc gcagcatagc gcgacaacgc ttccagcccc
    14821 agcgcgccgc ttccggcgaa gcaatccaga cattgggcgt caacaatgac cggagccagc
    14881 cagttaaaca acgtttcgcg tacgcggtcg gtggtggggc gcagacctgg gctatcagga
    14941 accgggagtt tacggcctcg ccactgcccg ccaataatgc ggatttggcc gctgccggaa
    15001 tgattcggtt ttttcatgat aaattgctca atccgccaga taacacatac ttgcaggcgg
    15061 tgatgtgaat taagttaagt gatagactat ttcatcattt ttttagctgc tatgtacata
    15121 gcgttaacgc tgtgccatga agcaacagcg aggagtgtag tcgcaaatgg cgaaagaaaa
    15181 aaaacgtggc tttttttcct ggctgggctt tggtcaaaaa gagcagaccc cggaaaaaga
    15241 gacagaagtt cagaatgaac aaccggttgt agaagaaatc gttcaggcgc aagagcctgt
    15301 gaaggcttcg gaacaagccg ttgaagagca gccgcaggcg catactgaag ccgaggcgga
    15361 aacttttgct gccgacgttg tggaagtcac tgaacaggtt gctgaaagtg aaaaagcgca
    15421 gcctgaagcg gaagtcgttg cacagccgga accggtcgca gaagaaacgc cggagccagt
    15481 ggctatcgaa cgtgaagagc tgccgttgcc agaagacgtc aacgccgaag aggtttcgcc
    15541 agaagagtgg caggctgaag cggaaaccgt agagattgtc gaagcggcag aagaagaagc
    15601 ggctaaagaa gaaatcaccg acgaagagct ggaagcacag gcgctggctg ccgaagcggc
    15661 agaagaggcg gtgatggtag ttcctccggc agaagaagag cagccggtgg aagaaattgc
    15721 ccaggagcaa gaaaaaccga ccaaagaagg tttcttcgcg cgcctgaaac gcagcctgtt
    15781 aaaaaccaaa gaaaatctcg gttccggatt tatcagcctg ttccgcggta aaaaaatcga
    15841 cgatgatctg tttgaggagc tggaagaaca gctgttgatc gccgatgtgg gcgtggaaac
    15901 cacacgtaaa attatcacca atctgacgga aggcgcatcc cgcaagcagc ttcgtgatgc
    15961 cgaggcgctc tatggcctgc tgaaagaaga gatgggcgag attctggcga aagtcgatga
    16021 gccgctgaat gttgaaggta aaacgccgtt cgtgatcctg atggtgggcg tcaacggtgt
    16081 gggtaaaact acgacgattg gtaagctggc acgtcagttt gagcagcagg gtaaatcggt
    16141 gatgctggcg gcgggcgata ctttccgtgc agcagcggtt gaacagcttc aggtctgggg
    16201 tcagcgcaac aatattccgg tgattgccca gcataccggt gcggattccg cctctgttat
    16261 cttcgacgcc attcaggcag ctaaagcgcg taatatcgac gtcctgattg ccgataccgc
    16321 cggacgcctg cagaacaaat cgcacctgat ggaagagttg aagaaaatcg tccgcgtgat
    16381 gaagaaactc gacgttgaag cgccgcatga agttatgctg actattgatg ccagtaccgg
    16441 gcagaatgcg gtaagccagg ccaaactgtt ccacgaagcc gttggcttaa ccggcatcac
    16501 gctaacgaaa ctggacggca cggcgaaagg cggggtaatt ttctcggtgg ctgaccagtt
    16561 tggtatccct atccgctaca ttggtgtcgg cgaacgtatt gaggatttgc gtccgtttaa
    16621 ggcggacgac tttatagagg cactttttgc ccgagaggat taacaatgat tcgctttgaa
    16681 catgtcagca aggcttatct cggtgggaga caggcgctgc agggcgttac gttccatatg
    16741 cagccgggtg agatggcgtt tctgaccggt cattccggcg cagggaaaag taccctcctg
    16801 aagctgatct gtgggattga gcggcccagc gccgggaaaa tctggtttag cggccatgac
    16861 atcacgcgtc tgaaaaaccg tgaagttccg tttctgcgcc gccagattgg catgattttc
    16921 caggatcacc atctactgat ggaccgtact gtctacgata acgtggcgat cccgctgatt
    16981 atcgctggtg ccagcggtga cgatattcgt cgccgggtgt cggcggcact ggataaagtc
    17041 gggctactgg acaaagcgaa gaacttccct attcagcttt ccggcggtga acaacagcgt
    17101 gttggcattg cccgcgcggt ggtgaataag cccgcggtac tgctggcgga cgaaccgacc
    17161 ggtaacctgg acgacgcgtt gtcggaaggg attctacgtc tgtttgaaga gtttaaccgc
    17221 gttggggtaa ccgtattgat ggcaacgcac gacatcaacc tgatttcacg gcgttcctat
    17281 cgcatgctca ccctgagcga tggtcacttg catggaggcg tgggccatga ataagcgcga
    17341 tgcaatcaat catattcggc agtttggcgg gcgtcttgat cgcttccgta aatcggtcgg
    17401 cggctctggc gacggcggtc gtaacgcccc gaaacgcgcg aaatcctcgc caaaaccggt
    17461 aaatcgtaaa accaacgttt tcaacgaaca ggtgcgctat gccttccacg gcgcattgca
    17521 ggatctgaaa agcaaaccgt tcgccacgtt tttaacggtg atggttatcg ccatctccct
    17581 gacgctgccc agcgtctgtt atatggtgta caaaaacgtt aatcaggcgg cgacgcagta
    17641 ttatccgtca ccgcaaatca ctgtttactt gcaaaaaacg ctggacgatg acgccgctgc
    17701 gggcgtggtg gcacagttgc aggccgagca aggcgtggag aaagtgaact atctttctcg
    17761 tgaagacgca ctgggcgagt tccgtaactg gtctggtttt ggtggtgcgc tggatatgct
    17821 ggaagaaaac ccgcttccgg cagtggcggt ggtgatcccg aaactcgatt ttcaggggac
    17881 ggaatcactc aatacgctgc gtgatcgtat cacgcagatt aacggcattg acgaagtgcg
    17941 gatggatgac agctggtttg cccgtctggc ggcgttgacc gggctggtcg ggcgcgtttc
    18001 ggcgatgatc ggcgtgttga tggtggcggc agtgttcctc gtcatcggca acagcgtacg
    18061 tctgagcatc tttgctcgcc gtgactccat caacgtgcag aaactgattg gtgcgacgga
    18121 tggtttcatc cttcgcccgt tcctgtatgg tggcgcactg ctgggatttt ctggcgcatt
    18181 gttgtcatta attttgtcag aaattctgat gctgcgattg tcatcggcgg ttgcggaagt
    18241 ggcacaggtt ttcggaacga agtttgatat caatggttta tcgttcgatg aatgcctgct
    18301 attgctgctg gtatgctcga tgattggctg ggtggcagcg tggcttgcca cggtacaaca
    18361 tttacgccac tttacgcctg aataataaaa gcgtgttata ctctttccct gcaatgggtt
    18421 ccgtagcagg gaaagagatc ccgttgtctc ttcccggtat ttcatctcta tgtcacattt
    18481 tgtgcgtaat ttattcacaa gcttgcattg aacttgtgga taaaatcacg gtctgataaa
    18541 acagtgaatg ataacctcgt tgctcttaag ctctggcaca gttgttgcta ccactgaagc
    18601 gccagaagat atcgattgag aggatttgaa tgactgacaa aatgcaaagt ttagctttag
    18661 ccccagttgg caacctggat tcctacatcc gggcagctaa cgcgtggccg atgttgtcgg
    18721 ctgacgagga gcgggcgctg gctgaaaagc tgcattacca tggcgatctg gaagcagcta
    18781 aaacgctgat cctgtctcac ctgcggtttg ttgttcatat tgctcgtaat tatgcgggct
    18841 atggcctgcc acaggcggat ttgattcagg aaggtaacat cggcctgatg aaagcagtgc
    18901 gccgtttcaa cccggaagtg ggtgtgcgcc tggtctcctt cgccgttcac tggatcaaag
    18961 cagagatcca cgaatacgtt ctgcgtaact ggcgtatcgt caaagttgcg accaccaaag
    19021 cgcagcgcaa actgttcttc aacctgcgta aaaccaagca gcgtctgggc tggtttaacc
    19081 aggatgaagt cgaaatggtg gcccgtgaac tgggcgtaac cagcaaagac gttcgtgaga
    19141 tggaatcacg tatggcggca caggacatga cctttgacct gtcttccgac gacgattccg
    19201 acagccagcc gatggcaccg gtgctctatc tgcaggataa atcatctaac tttgccgacg
    19261 gcatcgaaga tgataactgg gaagagcagg cggcaaaccg tctgaccgac gcgatgcagg
    19321 gtctggacga acgcagccag gacatcatcc gtgcgcgctg gctggacgaa gacaacaagt
    19381 ccacgttgca ggaactggct gaccgttacg gtgtttccgc tgaacgtgtg cgccaactgg
    19441 aaaagaacgc gatgaaaaaa cttcgcgccg ctattgaagc gtaatttccg ctattaagca
    19501 gagaaccctg gatgagagtc cggggttttt gttttttggg cctctacaat aatcaattcc
    19561 ccctccggca aaacgtcaat ccccacgcag attgttaata aactgtcaaa atagctattc
    19621 caatatcata aaaatggggt atgttttagc agagtatgct gctaaagcac gggtagtcat
    19681 gcataaaacg aaataaagtg ctgaatcaca acaccacaac acacgtaata accagaagaa
    19741 tggggattct caggatgaat ataaagggta aagcgttact ggcaggatgt atcgcgctgg
    19801 cattcagcaa tatggctctg gcagaagata ttaaagtcgc ggtcgtgggc gcaatgtccg
    19861 gtccggttgc gcagtacggt gaccaggagt ttaccggcgc agagcaggcg gttgcggata
    19921 tcaacgctaa aggcggcatt aaaggcaaca aactgcaaat cgtaaaatat gacgatgcct
    19981 gtgacccgaa acaggcggtt gcggtggcga acaaagtcgt taacgacggc attaaatatg
    20041 tgattggtca cctctgttct tcatcaacgc agcctgcgtc tgatatctac gaagacgaag
    20101 gcattttaat gatcacccca gcggcaaccg cgccggagct gaccgcccgt ggctatcagc
    20161 tgatcctgcg caccaccggc ctggactccg accaggggcc gacggcggcg aaatatattc
    20221 ttgagaaagt gaaaccgcag cgtattgcta tcgttcacga caaacagcaa tacggcgaag
    20281 gtctggcacg agcggtgcag gacggcctga agaaaggcaa tgctaacgtg gtgttctttg
    20341 atggtatcac cgccggggaa aaagatttct ctacgctggt ggcgcgtctg aagaaagaga
    20401 atatcgactt cgtttactac ggcggttacc acccggaaat ggggcaaatc ctgcgtcagg
    20461 cacgcgcggc agggctgaaa actcagttta tggggccgga aggtgtggct aacgtttcgc
    20521 tgtctaacat tgcgggcgaa tcagcggaag ggctgctggt gaccaagccg aagaactacg
    20581 atcaggttcc ggcaaacaaa cccattgttg acgcgatcaa agcgaaaaaa caggacccaa
    20641 gcggcgcatt cgtttggacc acctacgccg cgctgcaatc tttgcaggcg ggcctgaatc
    20701 agtctgacga tccggctgag atcgccaaat acctgaaagc gaactccgtg gataccgtaa
    20761 tgggaccgct gacgtgggat gagaaaggcg atctgaaagg ctttgagttc ggcgtatttg
    20821 actggcacgc caacggcacg gcgaccgatg cgaagtaatc attaatcggc aactttgggt
    20881 tgccgccaaa ttgctaatat cgagtacgtt gcttcatgcc ggatgcggcg taaacgcctt
    20941 atccggccta caagatccaa agaaatcagt aaattgcaac acacattgta ggcctgataa
    21001 gcgtagcgca tcaggcaatt tctcgttggt catcactttt gaaatcaact tgacgattaa
    21061 cgcttctccc agccgccctg ttgcgccgta aaccccagcg cctgcataaa cgccgtcatc
    21121 acaccgcgat cttccaagcc tgcatccgcc atccaccagc atgaaacgcc aggattgtta
    21181 cgcaaaacct cttccagcag atattgcccc acaccgcgac gtcgggtgac ttcccgcaca
    21241 cgcagggaat ccagtgctcc ctcggtgccg cttaaggtta cccgtacggc agcgagcagg
    21301 cgctcgttaa aacgcgcggc gtagatacgg tggttatcgt caacctgtaa agaggaaggg
    21361 gaatactccg gccagatctt ttgcaggtca atccggtcct ggtcgctaaa gttttctaat
    21421 cgaatgatgg tcagcttcat gggtaacccg tgtaaatcac aaaagtggaa ccagtgtagc
    21481 gaaataattt aatcggaggc tttctctttt ttatttcttt
//
LOCUS       DAZKHF020000060        21512 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2201S31241
            SAMN43267800-rid25106413.denovo.060, whole genome shotgun sequence.
ACCESSION   DAZKHF020000060 DAZKHF020000000
VERSION     DAZKHF020000060.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267800
            Sequence Read Archive: SRR30307263
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 21512)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21512)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 21512)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350581.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350581.2
            Genome Coverage       :: 151x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:21:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,652
            CDSs (total)                      :: 5,557
            Genes (coding)                    :: 5,320
            CDSs (with protein)               :: 5,320
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 237
            CDSs (without protein)            :: 237
            Pseudo Genes (ambiguous residues) :: 0 of 237
            Pseudo Genes (frameshifted)       :: 102 of 237
            Pseudo Genes (incomplete)         :: 130 of 237
            Pseudo Genes (internal stop)      :: 52 of 237
            Pseudo Genes (multiple problems)  :: 41 of 237
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21512
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267800-rid25106413.denovo.060"
                     /serotype="O 26:H11"
                     /isolate="2201S31241"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-01"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            complement(73..148)
                     /locus_tag="ACHOSC_RS09105"
                     /old_locus_tag="pgaptmp_001821"
     tRNA            complement(73..148)
                     /locus_tag="ACHOSC_RS09105"
                     /old_locus_tag="pgaptmp_001821"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(113..115),aa:Lys,seq:ttt)
     gene            complement(153..228)
                     /locus_tag="ACHOSC_RS09110"
                     /old_locus_tag="pgaptmp_001822"
     tRNA            complement(153..228)
                     /locus_tag="ACHOSC_RS09110"
                     /old_locus_tag="pgaptmp_001822"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(193..195),aa:Val,seq:tac)
     gene            complement(275..350)
                     /locus_tag="ACHOSC_RS09115"
                     /old_locus_tag="pgaptmp_001823"
     tRNA            complement(275..350)
                     /locus_tag="ACHOSC_RS09115"
                     /old_locus_tag="pgaptmp_001823"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(315..317),aa:Val,seq:tac)
     gene            complement(395..470)
                     /locus_tag="ACHOSC_RS09120"
                     /old_locus_tag="pgaptmp_001824"
     tRNA            complement(395..470)
                     /locus_tag="ACHOSC_RS09120"
                     /old_locus_tag="pgaptmp_001824"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(435..437),aa:Val,seq:tac)
     gene            729..2144
                     /gene="gltX"
                     /locus_tag="ACHOSC_RS09125"
                     /old_locus_tag="pgaptmp_001825"
     CDS             729..2144
                     /gene="gltX"
                     /locus_tag="ACHOSC_RS09125"
                     /old_locus_tag="pgaptmp_001825"
                     /EC_number="6.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004818 - glutamate-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--tRNA ligase"
                     /protein_id="HGU4547778.1"
                     /translation="MKIKTRFAPSPTGYLHVGGARTALYSWLFARNHGGEFVLRIEDT
                     DLERSTPEAIEAIMDGMNWLSLEWDEGPYYQTKRFDRYNAVIDQMLEEGTAYKCYCSK
                     ERLEALREEQMAKGEKPRYDGRCRHSHEHHADDEPCVVRFANPQEGSVVFDDQIRGPI
                     EFSNQELDDLIIRRTDGSPTYNFCVVVDDWDMEITHVIRGEDHINNTPRQINILKALK
                     APVPVYAHVSMINGDDGKKLSKRHGAVSVMQYRDDGYLPEALLNYLVRLGWSHGDQEI
                     FTREEMIKYFTLNAVSKSASAFNTDKLLWLNHHYINALPPEYVATHLQWHIEQENIDT
                     RNGPQLADLVKLLGERCKTLKEMAQSCRYFYEDFAEFDADAAKKHLRPVARQPLEVVR
                     DKLAAITDWTAENVHHAIQATADELEVGMGKVGMPLRVAVTGAGQSPALDVTVHAIGK
                     TRSIERINKALDFIAERENQQ"
     gene            complement(2196..2588)
                     /locus_tag="ACHOSC_RS09130"
                     /old_locus_tag="pgaptmp_001826"
     CDS             complement(2196..2588)
                     /locus_tag="ACHOSC_RS09130"
                     /old_locus_tag="pgaptmp_001826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416898.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfeC-like transcriptional regulator"
                     /protein_id="HGU4547779.1"
                     /translation="MKRLRNKMTTEELAECLGVAKQTVNRWIREKGWKTEKFPGVKGG
                     RARLILVDTQVCEFIQNTPAFHNTPMLMEAEERIAEYAPGARAPAYRQIINAIDNMTD
                     IEQEKVAQFLSREGIRNFLARLDIDESA"
     gene            complement(2590..2949)
                     /locus_tag="ACHOSC_RS09135"
                     /old_locus_tag="pgaptmp_001827"
     CDS             complement(2590..2949)
                     /locus_tag="ACHOSC_RS09135"
                     /old_locus_tag="pgaptmp_001827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311301.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfeC-like transcriptional regulator"
                     /protein_id="HGU4547780.1"
                     /translation="MFKERMTPDELARLTGYSRQTINKWVRKEGWTTSPKPGVQGGKA
                     RLVHVNEQVREYIRNAERPEGQGEAPALSGDAPLEVLLVTLAKEMTPVEQKQFTSLLL
                     REGIIGLLQRLGIRDSK"
     gene            3170..3245
                     /locus_tag="ACHOSC_RS09140"
                     /old_locus_tag="pgaptmp_001828"
     tRNA            3170..3245
                     /locus_tag="ACHOSC_RS09140"
                     /old_locus_tag="pgaptmp_001828"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:3203..3205,aa:Ala,seq:ggc)
     gene            3285..3360
                     /locus_tag="ACHOSC_RS09145"
                     /old_locus_tag="pgaptmp_001829"
     tRNA            3285..3360
                     /locus_tag="ACHOSC_RS09145"
                     /old_locus_tag="pgaptmp_001829"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:3318..3320,aa:Ala,seq:ggc)
     gene            3568..5757
                     /locus_tag="ACHOSC_RS09150"
                     /old_locus_tag="pgaptmp_001830"
     CDS             3568..5757
                     /locus_tag="ACHOSC_RS09150"
                     /old_locus_tag="pgaptmp_001830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416896.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MASE1 domain-containing protein"
                     /protein_id="HGU4547781.1"
                     /translation="MFVEHNLIKNIKIFTLAFTLTVVLIQLSRFISPLAIIHSSYIFL
                     AWMPLCVMLSILFIFGWRGVVPVLCGMFCTNLWNFHLSFLQTAVMLGSQTFVVLCACA
                     ILRWQLGTRWRYGLTSRYVWQRLFWLGLVTPIGIKCSMYLVGSFFDFPLKISTFFGDA
                     DAIFTVVDLLSLFTAVLIYNMLFYYLTRMIVSPHFAQILWRRDIAPSLGKEKRAFTLS
                     WLAALSVLLLLLCTPYENDFIAGYLVPVFFIIFTLGVGKLRYPFLNLTWAVSTLCLLN
                     YNQNFLQGVETEYSLAFILAVLISFSVCLLYMVRIYHRSEWLNRRWHLQALTDPLTLL
                     PNFRALEQAPEQEAGKSFCCLRIDNLEFMSRHYGLMMRVHCIRSICRTLLPLMQENEK
                     LYQLPGSELLLVLSGPETEGRLQHMVNILNSRQIHWNNTGLDMGYGAAWGRFDGNQET
                     LQPLLGQLSWLAEQSCAHHHVLALDSREEMVSGQTTKQVLLLNTIRTALDQGDLLLYA
                     QPIRNKEGDGYDEILARLKYDGGIMTPDKFLPLIAQFNLSARFDLQVLESLLKWLATH
                     PCDKKGPRFSVNLMPLTLLQKNIAGRIIRLFKRYHISPQAVILEITEEQAFSNAESSM
                     YNIEQLHKFGFRIAIDDFGTGYANYERLKRLQADIIKIDGIFVKDIVTNTLDAMIVRS
                     ITDLAKAKSLSVVAEFVETQQQQALLHKLGVQYLQGYLIGRPQPLAD"
     gene            complement(5807..7009)
                     /gene="nupC"
                     /locus_tag="ACHOSC_RS09155"
                     /old_locus_tag="pgaptmp_001831"
     CDS             complement(5807..7009)
                     /gene="nupC"
                     /locus_tag="ACHOSC_RS09155"
                     /old_locus_tag="pgaptmp_001831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside permease NupC"
                     /protein_id="HGU4547782.1"
                     /translation="MDRVLHFVLALAVVAILALLVSSDRKKIRIRYVIQLLVIEVLLA
                     WFFLNSDVGLGFVKGFSEMFEKLLGFANEGTNFVFGSMNDQGLAFFFLKVLCPIVFIS
                     ALIGILQHIRVLPVIIRAIGFLLSKVNGMGKLESFNAVSSLILGQSENFIAYKDILGK
                     ISRNRMYTMAATAMSTVSMSIVGAYMTMLEPKYVVAALVLNMFSTFIVLSLINPYRVD
                     ASEENIQMSNLHEGQSFFEMLGEYILAGFKVAIIVAAMLIGFIALIAALNALFATVTG
                     WFGYSISFQGILGYIFYPIAWVMGVPSSEALQVGSIMATKLVSNEFVAMMDLQKIAST
                     LSPRAEGIISVFLVSFANFSSIGIIAGAVKGLNEEQGNVVSRFGLKLVYGSTLVSVLS
                     ASIAALVL"
     gene            7345..8583
                     /locus_tag="ACHOSC_RS09160"
                     /old_locus_tag="pgaptmp_001832"
     CDS             7345..8583
                     /locus_tag="ACHOSC_RS09160"
                     /old_locus_tag="pgaptmp_001832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Nramp family divalent metal transporter"
                     /protein_id="HGU4547783.1"
                     /translation="MTNYRVESSSGRAARKMRLALMGPAFIAAIGYIDPGNFATNIQA
                     GASFGYQLLWVVVWANLMAMLIQILSAKLGIATGKNLAEQIRDHYPRPVVWFYWVQAE
                     IIAMATDLAEFIGAAIGFKLILGVSLLQGAVLTGIATFLILMLQRRGQKPLEKVIGGL
                     LLFVAAAYIVELIFSQPNLAQLGKGMVIPSLPTSEAVFLAAGVLGATIMPHVIYLHSS
                     LTQHLHGGSRQQRYSATKWDVAIAMTIAGFVNLAMMATAAAAFHFSGHTGVADLDEAY
                     LTLQPLLSHAAATVFGLSLVAAGLSSTVVGTLAGQVVMQGFIRFHIPLWVRRTVTMLP
                     SFIVILMGLDPTRILVMSQVLLSFGIALALVPLLIFTSDSKLMGDLVNSKRVKQTGWV
                     IVVLVVALNIWLLVGTALGL"
     gene            complement(8724..9050)
                     /gene="ypeC"
                     /locus_tag="ACHOSC_RS09165"
                     /old_locus_tag="pgaptmp_001833"
     CDS             complement(8724..9050)
                     /gene="ypeC"
                     /locus_tag="ACHOSC_RS09165"
                     /old_locus_tag="pgaptmp_001833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2502 domain-containing protein YpeC"
                     /protein_id="HGU4547784.1"
                     /translation="MFRSLFLAAALMAFTPLAANAGEITLLPSIKLQIGDRDHYGNYW
                     DGGHWRDRDYWHRNYEWRKNRWWRHDNGYHRGWDKRKAYERGYREGWRDRDDHRGKGR
                     GHGHRH"
     gene            complement(9165..10421)
                     /locus_tag="ACHOSC_RS09170"
                     /old_locus_tag="pgaptmp_001834"
     CDS             complement(9165..10421)
                     /locus_tag="ACHOSC_RS09170"
                     /old_locus_tag="pgaptmp_001834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005247 - voltage-gated chloride channel
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ion channel protein"
                     /protein_id="HGU4547785.1"
                     /translation="MLHPRARTMLLLSLPAVAIGIASSLILIVVMKIASVLQNLLWQR
                     LPGTLGIAQDSPLWIIGVLTLTGIAVGLVIRFSQGHAGPDPACEPLIGAPVPPSALPG
                     LIVALILGLAGGVSLGPEHPIMTVNIALAVAIGARLLPRVNRMEWTILASAGTIGALF
                     GTPVAAALIFSQTLNGSSEVPLWDRLFAPLMAAAAGALTTGLFFHPHFSLPIAHYGQM
                     EMTDILSGAIVAAIAIAAGMVAVWCLPRLHAMMHQMKNPVLVLGIGGFILGILGVIGG
                     PVSLFKGLDEMQQMVANQAFSTSDYFLLAVIKLAALVVAAASGFRGGRIFPAVFVGVA
                     LGLMLHEHVPAVPAAITVSCAILGIVLVVTRDGWLSLFMAAVVVPNTTLLPLLCIVML
                     PAWLLLAGKPMMMVNRPKQQPPHDNV"
     gene            10625..11590
                     /gene="glk"
                     /locus_tag="ACHOSC_RS09175"
                     /old_locus_tag="pgaptmp_001835"
     CDS             10625..11590
                     /gene="glk"
                     /locus_tag="ACHOSC_RS09175"
                     /old_locus_tag="pgaptmp_001835"
                     /EC_number="2.7.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004340 - glucokinase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0005536 - D-glucose binding [Evidence
                     IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA];
                     GO_process: GO:0051156 - glucose 6-phosphate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucokinase"
                     /protein_id="HGU4547786.1"
                     /translation="MTKYALVGDVGGTNARLALCDIASGEISQAKTYSGLDYPSLEAV
                     IRVYLEEHKVEVKDGCIAIACPITGDWVAMTNHTWAFSIAEMKKNLGFSHLEIINDFT
                     AVSMAIPMLKKEHLIQFGGAEPVEGKPIAVYGAGTGLGVAHLVHVDKRWVSLPGEGGH
                     VDFAPNSEEEAIILEILRAEIGHVSAERVLSGPGLVNLYRAIVKADNRLPENLKPKDI
                     TERALADSCTDCRRALSLFCVIMGRFGGNLALNLGTFGGVFIAGGIVPRFLEFFKASG
                     FRAAFEDKGRFKEYVYDIPVYLIVHDNPGLLGSGAHLRQTLGHIL"
     gene            11809..12135
                     /locus_tag="ACHOSC_RS09180"
                     /old_locus_tag="pgaptmp_001836"
     CDS             11809..12135
                     /locus_tag="ACHOSC_RS09180"
                     /old_locus_tag="pgaptmp_001836"
                     /EC_number="2.7.1.202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_function: GO:0022877 -
                     protein-N(PI)-phosphohistidine-fructose phosphotransferase
                     system transporter activity [Evidence IEA];
                     GO_function: GO:0090563 - protein-phosphocysteine-sugar
                     phosphotransferase activity [Evidence IEA];
                     GO_function: GO:0090582 -
                     protein-phosphocysteine-D-fructose-phosphotransferase
                     system transporter activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIB"
                     /protein_id="HGU4547787.1"
                     /translation="MSKKLIALCACPMGLAHTFMAAQALEEAAVEAGYEVKIETQGAD
                     GIQNRLTAQDIAEATIIIHSVAVTPEDNERFESRDVYEITLQDAIKNAAGIIKEIEEM
                     IASEQQ"
     gene            12157..13404
                     /locus_tag="ACHOSC_RS09185"
                     /old_locus_tag="pgaptmp_001837"
     CDS             12157..13404
                     /locus_tag="ACHOSC_RS09185"
                     /old_locus_tag="pgaptmp_001837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIC"
                     /protein_id="HGU4547788.1"
                     /translation="MAIKKRSATVVPGASGAAAAVKNPQASKSSFWGELPQHVMSGIS
                     RMVPTLIMGGVILAFSQLIAYSWLKIPAEIGIMDALNSGKFSGFDLSLLKFAWLSQSF
                     GGVLFGFAIPMFAAFVANSIGGKLAFPAGFIGGLMSTQPTQLLNFDPSTMQWATSSPV
                     PSTFIGALIISIVAGYLVKWMNQKIQLPDFLLAFKTTFLLPILSAIFVMLAMYYVITP
                     FGGWINGGIRTVLTAAGEKGALMYAMGIAAATAIDLGGPINKAAGFVAFSFTTDHVLP
                     VTARSIAIVIPPIGLGLATIIDRRLTGKGLFNAQLYPQGKTAMFLAFMGISEGAIPFA
                     LESPITAIPSYMVGAIVGSTAAVWLGAVQWFPESAIWAWPLVTNLGVYMAGIALGAVI
                     TALMVVFLRLMMFRKGKLLIDSL"
     gene            13419..14504
                     /gene="ypdF"
                     /locus_tag="ACHOSC_RS09190"
                     /old_locus_tag="pgaptmp_001838"
     CDS             13419..14504
                     /gene="ypdF"
                     /locus_tag="ACHOSC_RS09190"
                     /old_locus_tag="pgaptmp_001838"
                     /EC_number="3.4.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase"
                     /protein_id="HGU4547789.1"
                     /translation="MTLLASLRDWLKAQQLDAVLLSSRQNKQPHLGISTGSGYVVISR
                     ESAHILVDSRYFVEVEARAQGYQLHLLDATNTLTTIVNQIIADEQLQTLGFEGQQVSW
                     ETAHRWKSELNAKLVSATPDVLRQIKTPEEVEIIRLACGIADRGAEHIRRFIQAGMSE
                     REIAAELEWFMRQQGAEKASFDTIVASGWRGALPHGKASDKIVAAGEFVTLDFGALYQ
                     GYCSDMTRTLLVNGEGVSAESHPLFNVYQIVLQAQLAAISAIRPGVRCQQVDDAARQV
                     ITEAGFSHYFGHNTGHAIGIEVHEDPRFSPRDTTTLQPGMLLTVEPGIYLPGQGGVRI
                     EDVVLVTPQGAEVLYAMPKTVLLTGEA"
     gene            14504..15541
                     /gene="ypdE"
                     /locus_tag="ACHOSC_RS09195"
                     /old_locus_tag="pgaptmp_001839"
     CDS             14504..15541
                     /gene="ypdE"
                     /locus_tag="ACHOSC_RS09195"
                     /old_locus_tag="pgaptmp_001839"
                     /EC_number="3.4.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase"
                     /protein_id="HGU4547790.1"
                     /translation="MDLSLLKALSEADAIASSEQEVRQILLEEADRLQKEVRFDGLGS
                     VLIRLNESTGPKVMICAHMDEVGFMVRSISREGAIDVLPVGNVRMAARQLQPVRITTR
                     EECKIPGLLDGDRQGNDVSAMRVDIGARSYDEVMQAGIRPGDRVTFDTTFQVLPHQRV
                     MGKAFDDRLGCYLLVTLLRELHDAELPAEVWLVASSSEEVGLRGGQTATRAVSPDVAI
                     VLDTACWAKNFDYGAANHRQIGNGPMLVLSDKSLIAPPKLTAWIETVAAEIGVPLQAD
                     MFSNGGTDGGAVHLTGTGVPTVVMGPATRHGHCAASIADCRDILQMEQLLSALIQRLT
                     RETVVQLTDFR"
     gene            15566..18061
                     /gene="ptsP"
                     /locus_tag="ACHOSC_RS09200"
                     /old_locus_tag="pgaptmp_001840"
     CDS             15566..18061
                     /gene="ptsP"
                     /locus_tag="ACHOSC_RS09200"
                     /old_locus_tag="pgaptmp_001840"
                     /EC_number="2.7.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008965 - phosphoenolpyruvate-protein
                     phosphotransferase activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate--protein phosphotransferase"
                     /protein_id="HGU4547791.1"
                     /translation="MLTIQFLCPLPNGLHARPAWELKEQCSQWQSEITFFNHRQNAKA
                     DAKSSLALIGTGTLFNDSCSLNISGSDEEQARRVLEEYILVRFIDSDSVQPTQAELTA
                     HPLPRSLSRLNPDLLYGNVLASGVGVGTLTLLQSDSLDSYRAIPASAQDSTRLEHSLA
                     TLAEQLNQQLRERDGESKTILSAHLSLIQDDEFAGNIRRLMTEQHQGLGAAIISNMEQ
                     VCAKLSASASDYLRERVSDIRDISEQLLHITWPELKPRNNLVLEKPTILVAEDLTPSQ
                     FLSLDLKNLAGMILEKTGRTSHTLILTRASAIPVLSGLPLDAIARYAGQPAVLDAQCG
                     VLAINPNDAVSGYYQVAQTLADKRQKQQAQAAAQLAYSRDNKRIDIAANIGTALEAPG
                     AFANGAEGVGLFRTEMLYMDRDSAPDEQEQFEAYQQVLLAAGDKPIIFRTMDIGGDKS
                     IPYLNIPQEENPFLGYRAVRIYPEFAGLFRTQLRAILRAASFGNAQLMIPMVHSLDQI
                     LWVKGEIQKAIVELKRDGLRHAETITLGIMVEVPSVCYIIDHFCDEVDFFSIGSNDMT
                     QYLYAVDRNNPRVSPLYNPITPSFLRMLQQIVTTAHQRGKWVGICGELGGESRYLPLL
                     LGLGLDELSMSSPRIPAVKSQLRQLDSEACRELARQACECRSAQEIEALLTAFTPEED
                     VRPLLALENIFVDQDFSNKEQAIQFLCGNLGVNGRTEHPFELEEDVWQREEIVTTGVG
                     FGVAIPHTKSQWIRHSSISIARLAKPVDWQSEMGEVELVIMLTLGANEGMNHVKVFSQ
                     LARKLVNKNFRQSLFAAQDAQSILTLLETELTF"
     gene            complement(18064..18921)
                     /locus_tag="ACHOSC_RS09205"
                     /old_locus_tag="pgaptmp_001841"
     CDS             complement(18064..18921)
                     /locus_tag="ACHOSC_RS09205"
                     /old_locus_tag="pgaptmp_001841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HGU4547792.1"
                     /translation="MKAPGLPADQQFFADLFSGLVLNPQLLGRVWFASQPASLPVGSL
                     CIDFPRLDIVLRGEYGNLLEAKQQRMVEGEMLFIPARAANLPINNKPVMLLSLVFAPT
                     WLGLSFYDSRTTSLLHPARQIQLPSLQRGEGEAMLTALTHLSRSPLEQNIIQPLVLSL
                     LHLCRNVVNMPPGNSQPRGDFLYHSICNWVQDNYAQPLTRESVAQFFNITPNHLSKLF
                     AQHGTMRFIEYVRWVRMAKARMILQKYHLSIHEVAQRCGFPDSDYFCRVFRRQFGLTP
                     GEYSARFQG"
     gene            complement(18934..19668)
                     /gene="ypdB"
                     /locus_tag="ACHOSC_RS09210"
                     /old_locus_tag="pgaptmp_001842"
     CDS             complement(18934..19668)
                     /gene="ypdB"
                     /locus_tag="ACHOSC_RS09210"
                     /old_locus_tag="pgaptmp_001842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator YpdB"
                     /protein_id="HGU4547793.1"
                     /translation="MKVIIVEDEFLAQQELSWLIKEHSQMEIVGTFDDGLDVLKFLQH
                     NRVDAIFLDINIPSLDGVLLAQNISQFANKPFIVFITAWKEHAVEAFELEAFDYILKP
                     YQESRITGMLQKLEAAWQQQQTSSTPAATVTRENDTINLVKDERIIVTPINDIYYAEA
                     HEKMTFVYTRRESYVMPMNITEFCSKLPPSHFFRCHRSFCVNLNKIREIEPWFNNTYI
                     LRLKDLDFEVPVSRSKVKEFRQLMHL"
     gene            complement(19683..21380)
                     /gene="ypdA"
                     /locus_tag="ACHOSC_RS09215"
                     /old_locus_tag="pgaptmp_001843"
     CDS             complement(19683..21380)
                     /gene="ypdA"
                     /locus_tag="ACHOSC_RS09215"
                     /old_locus_tag="pgaptmp_001843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     YpdA"
                     /protein_id="HGU4547794.1"
                     /translation="MHEIFNMLLAVFDRAALMLICLFFLIRIRLFRELLHKSAHSPKE
                     LLAVTAIFSLFALFSTWSGVPVEGSLVNVRIIAVMSGGILFGPWVGIITGVIAGIHRY
                     LIDIGGVTAIPCFITSILAGCISGWINLKIPKAQRWRVGILGGMLCETLTMILVIVWA
                     PTTALGIDIVSKIGIPMILGSVCIGFIVLLVQSVEGEKEASAARQAKLALDIANKTLP
                     LFRHVNSESLRKVCEIIRDDIHADAVAITNTDHVLAYVGVGEHNYQNGDDFISPTTRQ
                     AMNYGKIIIKNNDEAHRTPEIHSMLVIPLWEKGVVTGTLKIYYCHAHQITSSLQEMAV
                     GLSQIISTQLEVSRAEQLREMANKAELRALQSKINPHFLFNALNAISSSIRLNPDTAR
                     QLIFNLSRYLRYNIELKDDEQIDIKKELYQIKDYIAIEQARFGDKLTVIYDIDEEVNC
                     CIPSLLIQPLVENAIVHGIQPCKGKGVVTISVAECGNRVRIAVRDTGHGIDPKVIERV
                     EANEMPGNKIGLLNVHHRVKLLYGEGLHIRRLEPGTEIAFYIPNQRTPVASQATLLL"
BASE COUNT         5117 a         5363 c         5732 g         5300 t
ORIGIN      
        1 aacctgcggc agcttcgaat ctttgagtat cgcagatagc aaaaaagcgc ctttagggcg
       61 ctttttttac attggtgggt cgtgcaggat tcgaacctgc gaccaattga ttaaaagtca
      121 actgctctac caactgagct aacgacccga agtggtgggt gatgacggga tcgaaccgcc
      181 gaccccctcc ttgtaaggga ggtgctctcc cagctgagct aatcacccac ttcggtactt
      241 cacattttta caagaaattt agctggcgag aaagtggtgg gtgatgacgg gatcgaaccg
      301 ccgaccccct ccttgtaagg gaggtgctct cccagctgag ctaatcaccc acttctcaat
      361 ttcttgctac acggcggaga ctacataaag tagttggtgg gtgatgacgg gatcgaaccg
      421 ccgaccccct ccttgtaagg gaggtgctct cccagctgag ctaatcaccc ccgctgtgtg
      481 gagtcgcatt atagggagag ttcaaaatga gtcaacgcat tttctaaaga aattgttcgt
      541 tcgtcgtaaa tttaagcaag atgatcgcaa aacagaccgt gttgcgcaat ttgtcaacga
      601 aaacaataat gcgtaaggtt gcaacccgaa ctacattgag gaatcaggcg ggagtgatag
      661 aatatcgccc actcaatttt tccaggattt gccggttgtc ggcatctttc taaacgtaag
      721 gccatttcat gaaaatcaaa actcgcttcg cgccaagccc aacaggctat ctgcacgttg
      781 gcggcgcgcg tactgctctt tactcctggc tttttgcacg taaccacggc ggtgagttcg
      841 tgctgcgtat tgaagacacc gatcttgagc gttccacgcc ggaagctatc gaagccatta
      901 tggatggcat gaactggctg agcctggagt gggatgaagg tccgtactac cagaccaaac
      961 gttttgatcg ctacaatgcg gtgatcgatc agatgctgga agagggcact gcttataaat
     1021 gctattgctc taaagagcgc ctggaagcgc tgcgcgaaga gcaaatggcg aaaggtgaga
     1081 agccgcgtta tgacggtcgc tgccgccaca gccatgagca tcatgctgat gatgaaccgt
     1141 gtgtcgtgcg ttttgctaat ccgcaggaag gttctgttgt ttttgacgat cagatccgtg
     1201 gtccgatcga gttcagcaac caggagctgg atgatctgat tatccgccgt accgatggtt
     1261 ccccaaccta taacttctgt gtggttgtcg atgactggga tatggaaatc acccacgtta
     1321 tccgtggcga agaccatatc aacaacacgc cgcgccagat caacattctt aaggccctga
     1381 aagcgccggt gccggtttac gcgcacgttt ctatgatcaa tggcgatgac ggtaaaaaac
     1441 tgtccaaacg tcacggggca gtcagcgtaa tgcagtatcg tgatgacggt tatttgccag
     1501 aagcactgct gaactatctg gtgcgtctgg gctggtccca cggcgatcag gaaatcttca
     1561 ctcgtgaaga gatgatcaaa tacttcactt tgaatgccgt cagcaaatct gccagtgcgt
     1621 tcaacaccga caagctgctg tggctgaacc atcactacat taacgcgctg ccgccggagt
     1681 atgttgctac tcacttacag tggcacattg agcaggaaaa tatcgatacc cgtaacggcc
     1741 cgcagctggc tgatctggtg aaactgctgg gcgaacgctg caagacgctg aaagagatgg
     1801 cacagagctg ccgttatttc tacgaagatt ttgctgagtt cgatgccgat gcggcgaaaa
     1861 aacatctgcg tccggtagcg cgtcagccgc tggaagtggt tcgtgacaaa ctggccgcga
     1921 ttactgactg gaccgctgaa aacgttcatc acgctattca ggcgacggcg gatgagctgg
     1981 aagtgggtat gggtaaagtt ggtatgccgc tgcgtgtcgc cgtaaccggt gcggggcagt
     2041 ctccagcact ggatgttacc gttcacgcaa ttggtaagac ccgcagtatc gagcgtatca
     2101 acaaagcgct ggattttatt gctgaacgcg aaaatcagca gtaattagcg tgtaaaaaga
     2161 taaacggcag gagataatat cctgccgttt tttatttatg ctgattcgtc aatatcgaga
     2221 cgggcgagga aattgcgaat tccttcacgt gacaaaaatt gcgcgacttt ttcctgctca
     2281 atgtctgtca tattatctat cgcgttgatg atttgtcgat acgccggggc gcgtgctcca
     2341 ggagcgtatt cagcaattct ctcttcagct tccatcaaca ttggtgtgtt atggaaggct
     2401 ggcgtgttct gaataaactc gcaaacttgc gtatcgacca gaataagtct ggcacgaccg
     2461 cctttcacgc cgggaaattt ttccgttttc cagccttttt ctctgatcca acggttaaca
     2521 gtttgtttag ccacaccgag gcattcagcc agttcttcgg tggtcatttt gttgcgtaat
     2581 cttttcatat tatttgctat cgcgaatccc taagcgttgt aacaatccga taatcccttc
     2641 ccgcagaagc aaggatgtaa actgtttttg ttcaactggc gtcatctctt tcgccagtgt
     2701 caccagcaat acttcaagcg gcgcatcacc agaaagggca ggcgcttctc cctgaccttc
     2761 tggacgttca gcattgcgaa tatattcacg aacctgttca ttgacgtgaa ccagtcgggc
     2821 tttgccaccc tggacgccag gttttggtga cgttgtccag ccttccttgc gtacccattt
     2881 attaatggtc tggcggctat agccggtcag tctggcaagt tcatctggtg tcatcctctc
     2941 cttgaacata acaattccct gaatgtatgt gggtctatta gtagttcatt ttataacacc
     3001 actttacagg aaaccgtgac atgaataact actgaatgcg agcggttttc caaaattgtt
     3061 catttgcctg atttttcagc gattgaaact acacgttgca atttgccgtt gacacattcg
     3121 ggcggaattc atatgatgcc gcccgtcaac tcgacaagct ttacgtgacg gggctatagc
     3181 tcagctggga gagcgcttgc atggcatgca agaggtcagc ggttcgatcc cgcttagctc
     3241 caccaaattt tgcacccagc aaatttggta cgtaaacgca tcgtggggct atagctcagc
     3301 tgggagagcg cttgcatggc atgcaagagg tcagcggttc gatcccgctt agctccacca
     3361 aatttccaac cctcgctgca aagcgggggt tttttgtctc tgctttttgc cgcttttgta
     3421 atacagtcta cgtccgggtt agcgccgcct ggtgaaagca tcattggatg aaaaatcggc
     3481 aacaggctgg cccctgtttg cttcgcgatg cgaataaact tattatttgt gtgcctgaaa
     3541 accccgatca gtgagagtag tgtactcatg tttgtggagc ataacctgat aaaaaatatc
     3601 aagatattca cactagcgtt tacgctcacc gtggtactta ttcagctatc ccgttttatt
     3661 tcgccacttg ccattatcca ttccagttat atctttctgg cgtggatgcc actgtgcgta
     3721 atgctgtcaa tcttgtttat ctttggctgg cgcggtgtcg ttcccgtttt atgcgggatg
     3781 ttttgcacca atctgtggaa ctttcatctc tcttttttac agactgcggt catgctcggt
     3841 agccagacgt ttgtcgtgtt gtgtgcctgc gcaatattac gctggcagct ggggacgcgt
     3901 tggcgttatg gattgaccag ccgatatgtc tggcaacgtc tgttctggct tggtttggtg
     3961 acgccgatcg gcatcaaatg cagcatgtat cttgtgggga gcttctttga tttcccgcta
     4021 aagatatcca cctttttcgg cgatgcggat gccattttca cggtcgttga tttgctaagc
     4081 cttttcaccg ctgtgctgat ttacaacatg cttttctact atctcactcg catgattgta
     4141 agtccccact ttgcgcagat attgtggcgc agggatatcg ctccgtcgtt gggcaaagag
     4201 aaacgcgcat ttaccttaag ctggctggca gcgttaagcg tgctgctact tctgttgtgc
     4261 acaccttatg aaaacgactt tattgccggt tacttggtac ccgttttctt catcatcttt
     4321 accctcgggg tcggtaagct tcgctatccg tttttaaatc tcacctgggc tgtttcaacg
     4381 ctttgcctgc tgaattacaa ccagaacttt ttgcaagggg tggaaaccga atattcgctg
     4441 gcatttattc ttgcggtgct gatttccttt agcgtttgcc tgctctatat ggtgcgcatt
     4501 tatcatcgca gtgaatggct taatcgccgc tggcatttgc aggcgctgac agatccgtta
     4561 acgctcctac ccaactttcg tgcgttggaa caagcgccgg agcaagaggc gggcaagagt
     4621 ttttgctgcc tgcgcattga taatcttgag tttatgagtc gtcattacgg cttaatgatg
     4681 cgcgttcact gtatccgctc aatttgccgt acgctgctgc cgttgatgca ggaaaacgaa
     4741 aagttgtatc aattgccggg tagtgaactg ctgttagtgc tgagcgggcc ggaaacggaa
     4801 gggcgactcc agcatatggt taacatcctg aatagtcggc aaattcactg gaacaatacc
     4861 gggctggata tgggctatgg tgctgcctgg gggcgttttg atggaaatca ggaaaccctg
     4921 caacccttgt tggggcagtt aagctggctg gcggagcaat cctgcgcaca tcatcatgtg
     4981 ctggcgctgg atagcagaga ggagatggtt tccgggcaga ccactaaaca ggtgctattg
     5041 ctgaatacca ttcgcacggc gttagatcag ggtgatttgc tgctctacgc ccagccaatt
     5101 cgcaacaaag agggtgacgg ttatgatgag atcctcgcgc gactgaaata tgacggcggc
     5161 attatgaccc cggataagtt tctgcccctt attgctcagt ttaaccttag cgcgcgtttt
     5221 gatttgcaag tgctggaatc cttgttgaag tggctggcaa cacacccttg cgacaaaaaa
     5281 ggaccgcgct tttcagtcaa tttaatgccg ctcacgctgc tgcaaaagaa tattgccggg
     5341 cggattattc gtctgtttaa gcgttatcac atctccccgc aggcggtcat tcttgagatc
     5401 accgaggagc aggcgttttc taacgcagaa agcagcatgt acaacatcga gcagctgcat
     5461 aagtttggtt tccggattgc gattgatgac tttggcaccg gatatgccaa ctacgaacgg
     5521 ttaaagcgtt tgcaggctga tatcatcaaa attgatggca tctttgtgaa agatattgtc
     5581 acgaatacgc tggatgcgat gattgtgaga tcaattaccg atctggcgaa agcgaagtca
     5641 ttgagtgtgg tcgcggagtt tgtcgagacg caacagcagc aggcgctatt gcataagctc
     5701 ggggtgcaat atctgcaagg gtatttgatt ggtcgcccgc agccattagc tgattaacag
     5761 gcgtaaaaaa accggggaat tatccccggc ttttttatgt atggtcttac agcaccagtg
     5821 ctgcgattga cgcagacagc acactcacca gggtagagcc gtaaaccagc ttcagaccga
     5881 agcgagaaac cacgttacct tgctcttcat tcaggccttt aactgcacct gcgataatcc
     5941 cgattgaaga gaagttagcg aaggaaacca ggaacacaga gatgatgcct tcagcacgcg
     6001 gagagagcgt ggaagcaatt ttctgcagat ccatcatcgc aacgaactcg ttggaaacca
     6061 gtttggtcgc catgatactg cccacttgca gtgcttcact ggaaggaaca cccatcaccc
     6121 atgcaatcgg atagaagatg tagcccagga tgccctggaa ggagatgctg tagccaaacc
     6181 agccagtaac ggtggcaaac agtgcgttca gcgcggcgat cagggcgata aagccgatca
     6241 gcatcgcggc aacgataatg gcaactttga aacctgccag aatgtattca cccagcattt
     6301 cgaagaagct ctgaccttcg tgcaggttgg acatctggat gttttcttca ctggcatcaa
     6361 cacggtaagg attgatcagc gacagcacga taaaggtgct gaacatgttc agtaccagcg
     6421 cagcaacgac gtatttcggt tccagcatgg tcatgtatgc accaacgatg gacatcgaca
     6481 cggtggacat tgccgtggca gccatggtgt acatacgatt acgggagatt ttgccgagga
     6541 tatctttata ggcaataaag ttttcagact gacccagaat cagggagctg acggcgttaa
     6601 aggattccag tttgcccatg ccgttgactt tggagagcag gaaaccaatt gcgcggatga
     6661 tcaccggcaa cacgcgaatg tgctggagaa taccgatcag tgcagagata aagacgattg
     6721 ggcacagcac tttcaggaag aagaatgcca ggccttgatc attcatgcta ccaaagacga
     6781 agttagtccc ttcgttggca aatccgagca gtttttcgaa catttcggag aagcctttca
     6841 cgaagcctaa accaacgtcg gagttcagga agaaccacgc cagtaacact tcgataacaa
     6901 gcagttgaat aacataacgg atacgaattt ttttgcggtc gctgcttacc agcagtgcga
     6961 gaatcgcaac aacggcaagt gccagtacaa aatgaaggac gcggtccata tttgctccaa
     7021 atatgaggca ggttaaattt ccgtgcacat tctatgtaac atggttaagg aaaacgagat
     7081 ctgacacaca ctataatgac gtcctgtgac aagcttcaaa aatagtgatc tgtcatacat
     7141 ttctgctatg ttgtgtatgg aagctgaaag ttatgtaaat gtgctaacat taaacataac
     7201 cataacattc aagtcatcaa tcttttcgta gtcataatcc tggtctatca aagaaatcac
     7261 cacaacccat tcaaatgaat ttgataatca ttctcgtttg gcatagcatg aaacatagca
     7321 aaggctatgt tttagaggca caagatgacg aactatcgcg ttgagagtag cagcggacgg
     7381 gcggcgcgca agatgaggct cgcattaatg ggacctgcgt tcattgcggc gattggttat
     7441 atcgatcccg gtaactttgc gaccaatatt caggcgggtg ctagcttcgg ctatcagcta
     7501 ctgtgggttg tcgtttgggc caacctgatg gcgatgctga ttcagatcct ctctgccaaa
     7561 ctagggattg ccaccggtaa aaatctggcg gagcagattc gtgatcacta tccgcgtccc
     7621 gtagtgtggt tctattgggt tcaggcagaa attattgcga tggcaaccga cctggcggaa
     7681 tttattggtg cggcgatcgg ttttaaactc attcttggtg tttcgttgtt gcagggcgcg
     7741 gtgctgacgg ggatcgcgac tttcctgatt ttaatgctgc aacgtcgcgg gcaaaaaccg
     7801 ctggagaaag tgattggcgg gttactgttg tttgttgccg cggcttacat tgtcgagttg
     7861 attttctccc agcctaacct ggcgcagctg ggtaaaggaa tggtgatccc gagtttacct
     7921 acttcggagg cggtcttcct ggcagcaggc gtgttagggg cgacgattat gccgcatgtg
     7981 atttatttgc actcttcgct cactcagcat ttacatggcg gttcgcgtca acaacgttat
     8041 tccgccacca aatgggatgt ggctatcgcc atgacgattg ccggttttgt caatctggcg
     8101 atgatggcta cagctgcggc ggcgttccac ttttctggtc atactggtgt tgccgatctt
     8161 gatgaggctt atctgacgct gcaaccgttg ttaagccatg ctgcggcaac ggtctttgga
     8221 ttaagcctgg ttgctgccgg actgtcctca acggtggtgg ggacactggc agggcaggtg
     8281 gtgatgcagg ggttcattcg cttccatatc ccgctgtggg tgcgtcgtac agtcaccatg
     8341 ctgccgtcat ttattgtcat tctgatggga ttagatccga cacggattct ggttatgagt
     8401 caggtgctgt taagttttgg tatcgccctg gcgctggttc cactgctgat tttcaccagt
     8461 gacagcaagt tgatgggcga tctggtgaac agcaaacgcg taaaacagac aggctgggtg
     8521 attgtagtgc tggtcgtggc gctaaatatc tggttgttgg tggggacggc gctgggattg
     8581 tagttgaatg agcgtcgcat ctggcactat tggcggatgc ggcgtaaacg ccttatccgc
     8641 cctacgcgtt aaagagccgg tttgtaggcc tgataagacg cgctagcgtc gcatcaggca
     8701 ttgtgctcca ttggaggacg cgtttaatgg cggtgcccat gtccgcggcc ttttccgcga
     8761 tgatcgtcac gatcgcgcca gccttcacga tagccacgct catatgcttt acgcttatcc
     8821 cagccacgat gatagccatt atcatgacgc caccagcggt ttttgcgcca ttcataatta
     8881 cgatgccagt aatcacgatc tcgccagtga ccaccgtccc agtaattacc gtaatgatcg
     8941 cgatcgccaa tttgtaattt gatcgatggc agtagggtga tttcacctgc atttgctgca
     9001 agcggggtaa atgccatcag ggcggccgcc agaaacagtg acctgaacat tgttattctc
     9061 cttcacgatc gaagccgtca gcggcctgtt aacgcaatat tacggggagg taaagccccg
     9121 tatcatcgcc ataactctta aatcataaag aagagcattt tttgctaaac attatcgtgg
     9181 ggtggctgtt gcttcggacg attgaccatc atcatcggct tacctgctaa taacagccat
     9241 gccggaagca tgacgataca gagcagcggt agcaatgtgg tattgggtac aacgactgcc
     9301 gccataaaaa gacttaacca gccatcgcgt gttaccacca gcacgatgcc gagaatagcg
     9361 caggaaacgg ttattgccgc tggaacggcg ggaacgtgct catgcagcat caaccctaat
     9421 gccacgccga caaacactgc cgggaagatt cgcccaccgc gaaagccact ggcggcagca
     9481 acgaccaggg cggcaagttt aattaccgcc agcaaaaagt aatcgctggt gctgaaagcc
     9541 tgatttgcca ccatctgctg catctcatcc agccctttaa acagcgaaac cggcccacca
     9601 ataactccca gaataccgag aataaatccg ccaataccta gcacgagcac cggatttttc
     9661 atttgatgca tcatcgcgtg caaccgtggt aagcaccata cggcgaccat tcctgctgcg
     9721 atggcaatcg ccgcgacaat tgcaccgctg agaatatcgg tcatttccat ctgtccgtaa
     9781 tgcgcaatgg gcagtgaaaa atggggatgg aaaaataatc cggtagtaag tgcaccagct
     9841 gctgccgcca ttaatggcgc aaaaagacga tcccatagcg gaacttcact actgccattt
     9901 aaggtttgcg aaaatatcaa cgccgccgca acaggtgtac caaacagcgc gccgatggtt
     9961 ccggcagagg ctaaaatagt ccactccatt cggttgacgc gcggtaacag acgagcgcca
    10021 atggcaaccg caagggcgat attgacggtc atgatcggat gttccggccc caggctgacg
    10081 ccgccagcaa gaccgagaat taatgctacg ataagtcctg gtagcgcaga gggcggaact
    10141 ggtgcgccga tcagcggttc acaggcgggg tctggtccgg catgaccctg gctgaaacgg
    10201 ataaccaacc ccaccgcaat acccgttagc gttaatacac cgatgatcca gagaggtgaa
    10261 tcctgggcta tccccagagt tcccggcagt cgttgccaga gcaaattctg taataccgag
    10321 gcgattttca tcaccacaat cagaataaga ctggacgcaa tcccaattgc cacggcgggg
    10381 agcgataata acaacatggt tctggctcgc ggatggagca tgttctattc cttatgcggg
    10441 gtcagatact tagtttgccc agcttgcaaa aaggcatcgc tgcaattggt gctgaaacga
    10501 taaagtaaat gtgtgaccca gatcgatatt tacagggagc ctgcctttcc ggcgttgttg
    10561 ttatgccccc aggtatttac agtgtgagaa agaattattt tgactttagc ggagcagttg
    10621 aagaatgaca aagtatgcat tagtcggtga tgtgggcggc accaacgcac gtcttgctct
    10681 gtgtgatatt gccagtggtg aaatctcgca ggctaagacc tattcagggc ttgattaccc
    10741 cagcctcgaa gcggtcattc gcgtttatct tgaagaacat aaggtcgagg tgaaagacgg
    10801 ctgtattgcc atcgcttgcc caattaccgg tgactgggtg gcaatgacca accatacctg
    10861 ggcgttctca attgccgaaa tgaaaaagaa tctcggtttt agccatctgg aaattattaa
    10921 cgattttacc gctgtatcga tggcgatccc gatgctgaaa aaagagcatc tgattcagtt
    10981 tggtggcgca gaaccggtcg aaggtaaacc tattgcggtt tacggtgccg gaacagggct
    11041 tggggttgcg catctggtcc atgtcgataa gcgttgggta agcttgccgg gcgaaggcgg
    11101 tcacgttgat tttgcgccga atagtgaaga agaggccatt atcctcgaaa tactgcgtgc
    11161 ggaaattggc catgtttcgg cggaacgcgt gctttctggc cctgggctgg tgaatttgta
    11221 tcgcgcaatt gtgaaagctg acaaccgcct gccagaaaat ctcaagccaa aagatattac
    11281 cgaacgtgcg ctggctgaca gctgcaccga ttgccgccgc gcgttgtcgc tgttttgcgt
    11341 cattatgggc cgttttggcg gcaatctggc gctcaatctc gggacatttg gcggcgtgtt
    11401 tattgcgggc ggtatcgtgc cgcgcttcct tgagttcttc aaagcctctg gtttccgtgc
    11461 cgcatttgaa gataaagggc gctttaaaga atatgtctat gatattccgg tgtatctcat
    11521 cgtccatgac aatccgggtc ttctcggttc cggtgcacat ttacgccaga ccttaggtca
    11581 cattctgtaa atccttcctg ttatatcggg aggtaactct cccgataatc ttttaaatca
    11641 tacagttaat tcaatttttc tttgtgtccc ctcacaagac cgacctgcgt cacacttccg
    11701 tacagcggga ttaattctcc agtaaatgca ttatttgtct ggtaacggcg atttgttttg
    11761 cacgttcata atttcactcg tcaactatca ctgaatgagg aattaacgat gagtaagaaa
    11821 ctaattgcct tatgtgcctg cccaatgggc ctggctcaca cttttatggc cgctcaggcg
    11881 ctggaagaag cggcggtaga agccggttat gaagtgaaaa ttgaaaccca gggcgcggac
    11941 ggtatccaga atcgcctgac ggcgcaggat atcgccgaag caaccatcat catccactcc
    12001 gtggcagtta ccccggaaga taatgagcgt ttcgaatcac gcgacgttta tgaaatcact
    12061 ttgcaggacg caattaaaaa cgctgcgggc atcatcaaag aaatcgaaga gatgattgcc
    12121 tctgaacaac agtaatattc gacagggata attgttatgg ccattaaaaa acgcagtgca
    12181 accgttgtgc ctggcgcatc cggtgcggca gcggcagtta agaatccgca ggcctctaaa
    12241 agcagcttct ggggtgaact cccgcagcat gtgatgtcag ggatttcacg catggtgccg
    12301 accttaatta tgggcggtgt gatcctcgct ttcagccagc tgattgctta tagctggctt
    12361 aaaattcctg ctgagatcgg catcatggat gctcttaata gcgggaaatt ctccggtttc
    12421 gacctctcct tactgaaatt tgcctggctg tcgcagtcct tcggcggcgt gttgtttggt
    12481 tttgccattc cgatgtttgc tgcttttgtg gcgaactcta tcggtggcaa actggcgttc
    12541 ccggctggtt ttatcggcgg cttgatgtct acccagccga cgcaactgct gaacttcgat
    12601 cccagcacta tgcaatgggc gacctcttcg ccggtgccgt ccaccttcat tggcgcgctg
    12661 attatttcta tcgttgccgg ttaccttgtg aaatggatga accagaaaat ccagttaccg
    12721 gatttcctgt tagcgttcaa aaccacattt ttgctaccga ttctttccgc catttttgtc
    12781 atgctggcga tgtactacgt catcaccccg tttggcggct ggatcaacgg cggtatccgt
    12841 actgtgctga ctgccgcagg tgagaagggc gcgctgatgt acgcaatggg gatcgccgcg
    12901 gcgacagcaa tcgaccttgg tggcccgatc aacaaagccg caggtttcgt tgccttcagc
    12961 tttaccactg accacgtatt accggtcacc gctcgttcta tcgctatcgt tattcctccg
    13021 attggtctgg gtctggcgac cattattgac cgtcgtttaa ccggcaaagg cctgttcaac
    13081 gctcaacttt atccgcaggg taagaccgcg atgttccttg cctttatggg gattagtgag
    13141 ggtgcgattc cgtttgcgct ggaaagcccc atcaccgcca ttccgtcgta tatggtcggc
    13201 gcgattgtcg gctcaaccgc cgctgtctgg ctgggcgcag tgcaatggtt cccggaatcc
    13261 gctatctggg catggccgct ggtcactaac ctgggcgtct atatggcggg gatcgcgctg
    13321 ggggcagtca taactgcgct gatggtggtg ttcctgcgtc tgatgatgtt ccgtaaaggc
    13381 aaattgttaa tcgatagcct gtaagaagga aaatccagat gacattactc gcttcgctgc
    13441 gcgactggct taaggcgcaa caactggatg cagtgcttct ctcctcacgg cagaacaaac
    13501 agccgcatct ggggatctcc accggatcag gctatgtggt tattagccgt gaaagtgcgc
    13561 acattctggt agattcgcgc tatttcgttg aggtggaagc ccgtgcgcaa ggctaccagc
    13621 tgcatttgct tgacgcgacg aacacgctta ccactatcgt caatcaaatc attgccgatg
    13681 agcagttgca aacgctcggt tttgaaggcc agcaggtgag ttgggaaacc gcgcaccgct
    13741 ggaagtctga actcaatgcg aaactggtta gcgccacgcc ggatgtgctg cggcaaatca
    13801 aaacgccaga ggaggtggag ataatccgcc tcgcctgtgg gattgccgat cgcggagcgg
    13861 agcatattcg ccgctttatt caggcgggga tgagcgagcg cgagatagcc gctgaactgg
    13921 agtggtttat gcgccagcag ggcgcagaaa aagcctcttt tgacaccatt gtcgccagcg
    13981 gctggcgcgg ggcgctgccg cacggcaaag ccagcgacaa gattgttgca gcgggcgagt
    14041 ttgtcactct cgattttggt gcgctgtatc agggctactg ctctgatatg acgcgcacct
    14101 tgctggtgaa tggcgaaggg gtgagcgccg aatctcaccc gctgtttaac gtctaccaga
    14161 ttgtcctgca ggcacagctc gcagcaatct ctgcaattcg ccccggcgtg cgctgccagc
    14221 aggttgacga tgccgcgcgc caagtgatta ccgaggcagg ttttagccac tatttcggtc
    14281 ataacaccgg tcatgctatc ggcattgaag ttcatgaaga tccgcgtttt tcaccgcggg
    14341 acaccacgac gctacagcca ggcatgttac tgaccgtgga gccggggatt tatttgccag
    14401 ggcaaggggg cgtgcgcatc gaagatgttg tgctggttac cccgcaaggc gcagaagtgc
    14461 tctacgccat gccgaaaaca gtgttgctca cgggagaggc ataatggatt tatcgctatt
    14521 aaaagcgttg agcgaggcag atgcgatcgc ctcctcggaa caggaagtgc ggcagatcct
    14581 gctggaagaa gcggatcgcc tgcaaaaaga agtgcgattt gatggtctgg gatcggtgct
    14641 gatccgcctc aatgaatcga caggtccgaa ggtgatgatc tgcgcccata tggacgaagt
    14701 gggatttatg gtgcgtagca tctcccgcga aggggcaatt gatgtgctgc cggttggcaa
    14761 cgtgcgcatg gctgcccgcc agctgcagcc ggtgcgcatc accacccgtg aagagtgcaa
    14821 aattccaggc ctgcttgacg gcgaccggca ggggaatgac gtcagcgcca tgcgcgtgga
    14881 cattggtgcg cgctcgtatg acgaagtgat gcaggcggga attcgtccag gcgatcgcgt
    14941 cacctttgat acaacttttc aggttctccc tcaccagcgg gtgatgggga aagcctttga
    15001 tgatcgcctc ggttgctatc tgctggtgac gttactacgt gagctgcacg atgccgaact
    15061 gcctgcggag gtgtggctgg tggcaagttc cagcgaagag gtgggattac gcggtgggca
    15121 aaccgctacc cgcgcggtgt cgccggacgt cgccattgtg ctggataccg cctgctgggc
    15181 gaaaaacttt gattatggcg cggctaacca tcgccagatt ggtaacggcc cgatgctggt
    15241 gttaagcgac aagtcactga ttgcgccgcc aaaacttacc gcctggatcg aaaccgtggc
    15301 ggctgaaatt ggcgtaccgt tgcaggcaga tatgttcagc aacggcggca cggacggcgg
    15361 agcggtgcac ttaaccggta ctggcgtacc cacagtggta atggggcctg ccacccgcca
    15421 cggacattgc gcggcatcga ttgccgattg ccgcgacatt ttgcagatgg agcaactttt
    15481 atctgccctt attcaacgtc ttacgcgtga gacggttgtt caactgacgg atttcagatg
    15541 atctcctgat taacccggag cggttatgtt aacgattcaa tttctctgtc ctttgcctaa
    15601 cggtctacat gctcgtccgg cgtgggaact taaagaacag tgcagccagt ggcaaagcga
    15661 aatcaccttt ttcaaccatc gccagaacgc aaaggccgat gctaaaagct cgctggcgtt
    15721 gattggaact ggcaccttat ttaatgacag ttgcagcctg aacattagcg gcagcgatga
    15781 agagcaggcg cggcgcgtgc tggaagagta catcctggtg cgctttatcg acagcgacag
    15841 cgttcagccg acgcaggcag aactgacggc gcatccgctg ccgcgttcat taagccgcct
    15901 taacccggat ttactgtacg gcaatgtgct ggcaagcggc gtcggcgtgg gtacgctgac
    15961 cctgttacaa agcgacagcc tcgacagtta tcgggcaatc cccgccagtg cgcaagattc
    16021 cacccggctg gagcacagcc tggcaacgct tgccgagcaa ctgaaccagc aattgcgtga
    16081 gcgtgacggc gaaagcaaaa ctatcctcag cgcccatttg tcgctgattc aggatgatga
    16141 attcgcgggc aatatccgtc gtctgatgac agaacagcat caagggctgg gggcggcgat
    16201 catcagcaat atggagcagg tttgcgccaa actttctgct tctgccagtg attatctgcg
    16261 cgaacgtgtt agcgacattc gcgatattag cgaacagttg ctgcatatca cctggccgga
    16321 actgaagccg cgcaacaatc tggtgctgga aaaaccgact attctggtag ccgaggattt
    16381 aaccccaagc cagtttttga gcctcgattt aaaaaatctt gcaggcatga ttctggagaa
    16441 aactgggcgc acctcgcata ccctgattct gacccgcgcc tcggcgatcc cggtactgag
    16501 cggcctgccg ctggatgcga ttgcccgtta tgccgggcaa ccggcggtgc ttgacgccca
    16561 gtgcggcgtg ctggcgatta acccgaatga cgcggtgagc ggttattatc aggtcgcaca
    16621 gacgctggcg gataaacgcc aaaaacaaca ggcgcaggcc gccgcgcagc tggcctattc
    16681 ccgtgataac aagcgtattg atattgcggc gaatatcggc accgctctgg aagcgccagg
    16741 cgcgtttgcc aacggcgcgg aaggcgttgg actgttccgt actgaaatgc tctatatgga
    16801 tcgcgacagc gcgccggacg agcaggagca atttgaagcc taccagcagg tgctactggc
    16861 agcgggcgac aagccgatta tcttccgcac aatggacatc ggtggcgata aaagcattcc
    16921 ttatctgaat attccccagg aagagaaccc gttcctcggc tatcgcgcgg tacgtattta
    16981 cccggaattt gctggcctgt tccgcactca actacgggcg attttgcgcg ccgccagttt
    17041 tggcaacgcc cagttgatga tcccgatggt ccacagtctc gatcagatct tatgggtgaa
    17101 gggcgagatc caaaaagcga tcgttgagct taagcgcgat ggcctgcgtc atgcagagac
    17161 gattacgctg gggatcatgg tggaagtgcc gtcggtgtgc tacatcatcg atcacttctg
    17221 cgatgaggtc gatttcttca gtatcggctc caacgatatg acccagtatc tgtatgcggt
    17281 cgatcgtaat aacccgcgcg tatcgccgct gtataacccg attacgccat cgttcctgcg
    17341 catgttgcag cagattgtta ccactgcgca tcagcggggc aaatgggtag gcatttgcgg
    17401 tgaactgggc ggtgaaagcc gttatctgcc gctactgctt gggctgggcc tggatgagct
    17461 gagtatgagt agcccgcgta ttccggcggt gaaaagccag cttcgtcaac tggatagcga
    17521 ggcgtgtcgg gaactggcgc gtcaggcatg tgaatgccgc agtgcgcagg aaattgaagc
    17581 gttactcacc gcctttacgc cggaagaaga cgttcgccca ctgctggcgc tggagaatat
    17641 ctttgttgat caggatttta gcaataaaga gcaggcgatc cagttcctgt gcggcaacct
    17701 cggcgttaac gggcgcactg aacatccgtt tgagctggaa gaagatgtct ggcagcggga
    17761 agagattgtc accaccggcg ttggttttgg cgtagcgatc ccgcacacca aatctcagtg
    17821 gatccgtcat tccagtatca gcattgcccg gctggcgaaa ccggttgact ggcagtcaga
    17881 aatgggcgaa gtcgaactgg tgatcatgct gacgctgggt gctaacgaag ggatgaatca
    17941 tgtgaaagtc ttctcgcagc tggcgcgtaa actggtgaat aaaaacttcc gccagtcgtt
    18001 gtttgccgcg caagatgcac aaagtatcct gacgctgctg gaaacagaat taaccttctg
    18061 acgttagccc tgaaaacggg cgctgtactc tcccggcgtc agaccaaact gacgccggaa
    18121 aacgcgacaa aaatagtcgc tatccggaaa accgcaacgc tgtgccactt catgaattga
    18181 cagatgatat ttctgcaaaa tcatcctcgc cttcgccatt cgcacccaac gcacatactc
    18241 gataaaacgc atcgttccat gctgagcaaa cagttttgac agatgattgg gcgtgatatt
    18301 aaaaaactgc gccacgctct cgcgggtgag cggctgggca taattatcct gaacccagtt
    18361 acaaatgctg tgatagagaa aatcgccgcg cggttgcgaa ttacccggcg gcatattcac
    18421 cacgttgcgg caaagatgca gcaaacttaa caccagtggc tgaatgatat tctgctccag
    18481 cggcgaacgg ctaagatggg tgagggcggt aagcatcgct tcaccttcac cgcgttgcag
    18541 gctgggaagc tggatctggc gagcaggatg caacaacgac gtggtgcggc tatcgtaaaa
    18601 cgataaccca agccaggtcg gtgcgaacac caggcttaac agcatcaccg gtttgttgtt
    18661 gatcggtaaa ttagccgcgc gtgccggaat aaacagcatt tctccttcca ccattcgctg
    18721 ctgctttgct tccagcaggt tgccgtattc gccgcgcagc acgatatcca gacggggaaa
    18781 atcaatacat aaactgccca ccggtaacga ggcaggctgg ctggcaaacc agacgcgccc
    18841 cagtagttgc gggttaagca ccaggccgct gaacagatcg gcgaaaaatt gttgatcggc
    18901 gggcaagcct ggtgctttca tgaaagttcc cgattaaaga tgcattaact ggcgaaattc
    18961 tttcactttg ctgcgactga ccggcacttc aaaatccaga tctttcagtc gcagaatgta
    19021 ggtgttatta aaccacggtt cgatttcgcg tattttgttc agattgacac aaaatgagcg
    19081 atggcagcgg aaaaaatgcg acggcggcag tttgctgcaa aattcggtaa tgttcatcgg
    19141 cattacgtag gattcacgcc gcgtatagac aaaggtcatt ttctcgtgcg cttcggcgta
    19201 atagatatcg ttaattggcg tgacgattat tcgctcatct ttcaccagat taatggtgtc
    19261 attctcacgc gttaccgtcg cggcaggcgt actgctggtc tgctgttgtt gccaggccgc
    19321 ttccagtttt tgcagcatcc cggtaatacg tgactcctga tacggtttga gaatgtagtc
    19381 aaacgcctcc agttcaaacg cttctaccgc atgttctttc cacgcggtga tgaacacaat
    19441 aaacggttta ttggcgaact ggctgatgtt ttgcgccagc aacacgccat ccagcgacgg
    19501 aatattgata tccagaaaaa tggcgtcgac gcggttatgc tgcaaaaact tcaacacgtc
    19561 cagaccgtcg tcaaaggtgc cgacaatctc catctggctg tgctctttaa ttagccagct
    19621 cagttcctgt tgtgccagga attcgtcttc aacaatgatg actttcacaa tatcactccg
    19681 gctcaaagca ataacgtagc ctgtgaggcg actggggtgc gttggttagg aatgtaaaac
    19741 gcaatttccg tccccggctc caggcggcgg atatgcagcc cctcgccata caataacttc
    19801 acgcgatgat ggacattcag caggccaatt ttattgcccg gcatttcatt cgcttcgacc
    19861 cgctcaatca cctttggatc gatgccgtgc ccggtatctc gcaccgcaat gcgtacccga
    19921 tttccgcact ctgcaacgct gatggtgaca acgcctttac ctttgcaagg ctgaataccg
    19981 tggacaatgg cgttctccac caacggctgg atcagcaggc tgggaatgca gcaattcacc
    20041 tcttcatcaa tatcatagat aaccgtcagc ttgtcaccaa agcgggcctg ctcaatggca
    20101 atatagtctt taatttgata cagctctttt ttgatatcga tttgctcatc gtcttttaat
    20161 tcaatgttat agcgcagata acgcgacaga ttaaagatca actggcgagc ggtatccgga
    20221 ttcagacgga ttgacgatga aatagcgttc agagcgttaa acagaaaatg gggattaatt
    20281 ttgctttgca gggcgcgaag ctctgcctta tttgccattt cacgtagctg ctcggcgcgt
    20341 gaaacctcca gttgcgtgga gatgatttgc gacagaccga ccgccatttc ctgtaatgac
    20401 gaggtgatct gatgcgcgtg gcagtagtaa attttcagcg ttccggtcac gacccctttc
    20461 tcccacaatg ggatcaccag catggaatga atctctggtg tgcggtgggc ttcatcattg
    20521 tttttaatga tgatttttcc gtaattcatc gcctgacggg tagtcgggct aatgaagtca
    20581 tcgccattct gatagttatg ttcacccacg ccaacatagg ccagcacatg atcggtatta
    20641 gtaatcgcca ccgcatcggc gtgaatgtca tcgcggataa tttcgcagac cttgcgtaat
    20701 gactcgctat tgacatggcg aaacagcggt agcgttttgt tggcgatatc cagcgccagc
    20761 ttggcctgcc gcgcggcgct ggcctctttt tcgccctcaa cgctttgcac cagaagcaca
    20821 ataaagccga tacagacgct accgaggatc attggaatgc cgattttaga gacgatatcg
    20881 atccccaacg cggtagttgg tgcccagaca attaccagaa tcatggtcaa cgtctcacac
    20941 aacatgccgc cgagaatacc gacgcgccag cgctgtgctt tggggatttt caggttgatc
    21001 cagcccgata tacaacccgc cagaatgctg gtgataaagc aggggatcgc cgtcacgccg
    21061 ccgatatcaa ttaaataccg gtgaataccc gcaatcacgc cggtaatgat gcctacccac
    21121 gggccaaaca gaatcccgcc ggacatcacc gcgataatac gtacgttcac cagcgagcct
    21181 tctacgggaa cgccggacca ggtgctgaac agggcgaaca gcgagaaaat ggcggtaacg
    21241 gcgagcaatt cttttgggga gtgcgccgac ttgtgcaaca gttcgcgaaa caggcggata
    21301 cggatgagaa agaacaggca gataagcatt aacgctgccc gatcgaagac cgccagcagc
    21361 atgttgaata tttcgtgcac gggtgactct gaataaggat taaacctcta tgataggtaa
    21421 cctgaaggct gatgaccagc aggccgtttt tgaggaaaaa ccataaattt aaaaggggat
    21481 tgaaagataa atggtgtatt agcaatgtat ct
//
LOCUS       DAZKHF020000061        21270 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2201S31241
            SAMN43267800-rid25106413.denovo.061, whole genome shotgun sequence.
ACCESSION   DAZKHF020000061 DAZKHF020000000
VERSION     DAZKHF020000061.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267800
            Sequence Read Archive: SRR30307263
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 21270)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21270)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 21270)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350581.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350581.2
            Genome Coverage       :: 151x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:21:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,652
            CDSs (total)                      :: 5,557
            Genes (coding)                    :: 5,320
            CDSs (with protein)               :: 5,320
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 237
            CDSs (without protein)            :: 237
            Pseudo Genes (ambiguous residues) :: 0 of 237
            Pseudo Genes (frameshifted)       :: 102 of 237
            Pseudo Genes (incomplete)         :: 130 of 237
            Pseudo Genes (internal stop)      :: 52 of 237
            Pseudo Genes (multiple problems)  :: 41 of 237
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21270
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267800-rid25106413.denovo.061"
                     /serotype="O 26:H11"
                     /isolate="2201S31241"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-01"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            complement(306..1094)
                     /gene="phoH"
                     /locus_tag="ACHOSC_RS09220"
                     /old_locus_tag="pgaptmp_001844"
     CDS             complement(306..1094)
                     /gene="phoH"
                     /locus_tag="ACHOSC_RS09220"
                     /old_locus_tag="pgaptmp_001844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001741490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate starvation-inducible protein PhoH"
                     /protein_id="HGU4547795.1"
                     /translation="MGRQKAVIKARREAKRVLRRDSRSHKQREEESVTSLVQMGGVEA
                     IGMARDSRDTSPILARNEAQLHYLKAIESKQLIFATGEAGCGKTWISAAKAAEALIHK
                     DVDRIIVTRPVLQADEDLGFLPGDIAEKFAPYFRPVYDVLVRRLGASFMQYCLRPEIG
                     KVEIAPFAYMRGRTFENAVVILDEAQNVTAAQMKMFLTRLGENVTVIVNGDITQCDLP
                     RGVCSGLSDALERFEEDEMVGIVRFGKEDCVRSALCQRTLHAYS"
     gene            complement(1713..2984)
                     /gene="efeB"
                     /locus_tag="ACHOSC_RS09225"
                     /old_locus_tag="pgaptmp_001845"
     CDS             complement(1713..2984)
                     /gene="efeB"
                     /locus_tag="ACHOSC_RS09225"
                     /old_locus_tag="pgaptmp_001845"
                     /EC_number="4.98.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004601 - peroxidase activity [Evidence
                     IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0033212 - iron import into cell [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron uptake transporter
                     deferrochelatase/peroxidase subunit"
                     /protein_id="HGU4547796.1"
                     /translation="MQYEDKNGVNEPSRRRLLKGIGALALAGSCPVAHAQKTQSAPGT
                     LSPDARNEKQPFYGEHQAGILTPQQAAMMLVAFDVLASDKADLERLFRLLTQRFAFLT
                     QGGAAPETPNPRLPPLDSGILGGYIAPDNLTITLSVGHSLFDERFGLAPQMPKKLQKM
                     TRFPNDSLDAALCHGDVLLQICANTQDTVIHALRDIIKHTPDLLSVRWKREGFISDHA
                     ARSKGKETPINLLGFKDGTANPDSQNDKLMQKVVWVTADQQEPAWTIGGSYQAVRLIQ
                     FRVEFWDRTPLKEQQTIFGRDKQTGAPLGMQHEHDVPDYASDPEGKVIALDSHIRLAN
                     PRTAESESSLMLRRGYSYSLGVTNSGQLDMGLLFVCYQHDLEKGFLTVQKRLNGEALE
                     EYVKPIGGGYFFALPGVKDANDYLGSALLRV"
     gene            complement(2990..4117)
                     /gene="efeO"
                     /locus_tag="ACHOSC_RS09230"
                     /old_locus_tag="pgaptmp_001846"
     CDS             complement(2990..4117)
                     /gene="efeO"
                     /locus_tag="ACHOSC_RS09230"
                     /old_locus_tag="pgaptmp_001846"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron uptake system protein EfeO"
                     /protein_id="HGU4547797.1"
                     /translation="MTINFRRNALQLSVAALFSSAFMANAADVTQVKVTVTDKQCEPM
                     TITVNAGKTQFIIQNHSQKALEWEILKGVMVVEERENIAPGFSQKMTANLQPGEYDMT
                     CGLLTNPKGKLIVKGEATADAAQSDALLSLGGAITAYKAYVMAETTQLVTDTKAFTDA
                     IKAGDIEKAKALYAPTRQHYERIEPIAELFSDLDGSIDAREDDYEQKAADPKFTGFHR
                     LEKALFGDNTTKGMDQYADQLYTDVVDLQKRISELAFPPSKVVGGAAGLIEEVAASKI
                     SGEEDRYSHTDLWDFQANVEGSQKIVDLLRPQLQKANPELLAKVDANFKKVDTILAKY
                     RTKDGFETYDKLTDADRNALKGPITALAEDLAQLRGVLGLD"
     gene            complement(4175..5005)
                     /gene="efeU"
                     /locus_tag="ACHOSC_RS09235"
                     /old_locus_tag="pgaptmp_001847"
     CDS             complement(4175..5005)
                     /gene="efeU"
                     /locus_tag="ACHOSC_RS09235"
                     /old_locus_tag="pgaptmp_001847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0033573 - high-affinity iron permease
                     complex [Evidence IEA];
                     GO_function: GO:0005381 - iron ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0034755 - iron ion transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron uptake transporter permease EfeU"
                     /protein_id="HGU4547798.1"
                     /translation="MFVPFLIMLREGLEAALIVSLIASYLKRTQRGRWIGVMWIGVLL
                     AAALCLGLGIFINETTGEFPQKEQELFEGIVAVIAVVILTWMVFWMRKVSRNVKVQLE
                     QAVDSALQRGNHHGWALVMMVFFAVAREGLESVFFLLAAFQQDVGIWPPLGAMLGLAT
                     AVVLGFLLYWGGIRLNLGAFFKWTSLFILFVAAGLAAGAIRAFHEAGLWNHFQEIAFD
                     MSAVLSTHSLFGTLMEGIFGYQEAPSVSEVAVWFIYLIPALVAFALPPRAGATASRSA
                     "
     gene            complement(5671..7179)
                     /gene="putP"
                     /locus_tag="ACHOSC_RS09240"
                     /old_locus_tag="pgaptmp_001848"
     CDS             complement(5671..7179)
                     /gene="putP"
                     /locus_tag="ACHOSC_RS09240"
                     /old_locus_tag="pgaptmp_001848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005298 - proline:sodium symporter
                     activity [Evidence IEA];
                     GO_function: GO:0031402 - sodium ion binding [Evidence
                     IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA];
                     GO_process: GO:0015824 - proline transport [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sodium/proline symporter PutP"
                     /protein_id="HGU4547799.1"
                     /translation="MAISTPMLVTFCVYIFGMILIGFIAWRSTKNFDDYILGGRSLGP
                     FVTALSAGASDMSGWLLMGLPGAVFLSGISESWIAIGLTLGAWINWKLVAGRLRVHTE
                     YNNNALTLPDYFTGRFEDKSRILRIISALVILLFFTIYCASGIVAGARLFESTFGMSY
                     ETALWAGAAATILYTFIGGFLAVSWTDTVQASLMIFALILTPVIVIISVGGFGDSLEV
                     IKQKSIENVDMLKGLNFVAIISLMGWGLGYFGQPHILARFMAADSHHSIVHARRISMT
                     WMILCLAGAVAVGFFGIAYFNEHPAVAGAVNQNAERVFIELAQILFNPWIAGILLSAI
                     LAAVMSTLSCQLLVCSSAITEDLYKAFLRKQASQKELVWVGRVMVLVVALVAIALAAN
                     PENRVLGLVSYAWAGFGAAFGPVVLFSVMWSRMTRNGALAGMIIGALTVIVWKQFGWL
                     GLYEIIPGFIFGSIGIVVFSLLGKAPSAAMQKRFAEADAHYHSAPPSRLQES"
     gene            complement(7338..7547)
                     /locus_tag="ACHOSC_RS09245"
                     /old_locus_tag="pgaptmp_001849"
     CDS             complement(7338..7547)
                     /locus_tag="ACHOSC_RS09245"
                     /old_locus_tag="pgaptmp_001849"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4547800.1"
                     /translation="MMLQLCATMLNVTCVASLKMNEMLIKEIDMTGIKKITQTFSLRQ
                     LTFLKGATAKNVRECNLMKNSVAEH"
     gene            7602..11564
                     /gene="putA"
                     /locus_tag="ACHOSC_RS09250"
                     /old_locus_tag="pgaptmp_001850"
     CDS             7602..11564
                     /gene="putA"
                     /locus_tag="ACHOSC_RS09250"
                     /old_locus_tag="pgaptmp_001850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0003842 - 1-pyrroline-5-carboxylate
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0004657 - proline dehydrogenase activity
                     [Evidence IEA];
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trifunctional transcriptional regulator/proline
                     dehydrogenase/L-glutamate gamma-semialdehyde
                     dehydrogenase"
                     /protein_id="HGU4547801.1"
                     /translation="MGTTTMGVKLDDATRERIKSAATRIDRTPHWLIKQAIFSYLEQL
                     ENSDTLPELPALLSGAANESDEAPTPAEEPHQPFLDFAEQILPLSVSRAAITAAYRRP
                     ETEAVSMLLEQARLPQPVAEQAHKLAYQLADKLRNQKNASGRAGMVQGLLQEFSLSSQ
                     EGVALMCLAEALLRIPDKATRDALIRDKISNGNWQSHIGRSPSLFVNAATWGLLFTGK
                     LVSTHNEASLSRSLNRIIGKSGEPLIRKGVDMAMRLMGEQFVTGETIAEALANARKLE
                     EKGFRYSYDMLGEAALTAADAQAYMVSYQQAIHAIGKASNGRGIYEGPGISIKLSALH
                     PRYSRAQYDRVMEELYPRLKSLTLLARQYDIGINIDAEEADRLEISLDLLEKLCFEPE
                     LAGWNGIGFVIQAYQKRCPLVIDYLIDLATRSRRRLMIRLVKGAYWDSEIKRAQMDGL
                     EGYPVYTRKVYTDVSYLACAKKLLAVPNLIYPQFATHNAHTLAAIYQLAGQNYYPGQY
                     EFQCLHGMGEPLYEQVTGKVADGKLNRPCRIYAPVGTHETLLAYLVRRLLENGANTSF
                     VNRIADTSLPLDELVADPVTAVEKLAQQEGQTGLPHPKIPLPRDLYGHGRDNSAGLDL
                     ANEHRLASLSSALLNSALQKWQALPMLEQPVAAGEMSPVINPAEPKDIVGFVREATPR
                     EVEQALESAVNNAPIWFATPPVERAAILHRAAVLMESQMQQLIGILVREAGKTFSNAI
                     AEVREAVDFLHYYAGQVRDDFANETHRPLGPVVCISPWNFPLAIFTGQIAAALAAGNS
                     VLAKPAEQTPLIAAQGIAILLEAGVPPGVVQLLPGQGETVGAQLTGDDRVRGVMFTGS
                     TEVATLLQRNIASRLDAQGRPIPLIAETGGMNAMIVDSSALTEQVVVDVLASAFDSAG
                     QRCSALRVLCLQDEIADHTLKMLRGAMAECRMGNPGRLTTDIGPVIDSEAKANIERHI
                     QTMRSKGRPVFQAVRENSEDAREWQSGTFVAPTLIELDDFAELQKEVFGPVLHVVRYN
                     RNQLPELIEQINASGYGLTLGVHTRIDETIAQVTGSAHVGNLYVNRNMVGAVVGVQPF
                     GGEGLSGTGPKAGGPLYLYRLLANRPESALAVTLARQDAEYPVDAQLKAALTQPLNAL
                     REWAANRPELQALCTQYGELAQAGTQRLLPGPTGERNTWTLLPRERVLCIADDEQDAL
                     TQLAAVLAVGSQVLWPDDALHRQLVKALPSAVSERIQLAKAENITAQPFDAVIFHGDS
                     DQLRALCEAVAARDGAIVSVQGFARGESNILLERLYIERSLSVNTAAAGGNASLMTIG
                     "
     gene            complement(11604..12242)
                     /gene="rutR"
                     /locus_tag="ACHOSC_RS09255"
                     /old_locus_tag="pgaptmp_001851"
     CDS             complement(11604..12242)
                     /gene="rutR"
                     /locus_tag="ACHOSC_RS09255"
                     /old_locus_tag="pgaptmp_001851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator RutR"
                     /protein_id="HGU4547802.1"
                     /translation="MTQGAVKTTGKRSRAVSAKKKAILSAALDTFSQFGFHGTRLEQI
                     AELAGVSKTNLLYYFPSKEALYIAVLRQILDIWLAPLKAFREDFAPLAAIKEYIRLKL
                     EVSRDYPQASRLFCMEMLAGAPLLMDELTGDLKALIDEKSALIAGWVKSGKLAPIDPQ
                     HLIFMIWASTQHYADFAPQVEAVTGATLRDEIFFNQTVENVQRIIIEGIRPR"
     gene            12530..13621
                     /gene="rutA"
                     /locus_tag="ACHOSC_RS09260"
                     /old_locus_tag="pgaptmp_001852"
     CDS             12530..13621
                     /gene="rutA"
                     /locus_tag="ACHOSC_RS09260"
                     /old_locus_tag="pgaptmp_001852"
                     /EC_number="1.14.99.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309285.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine utilization protein A"
                     /protein_id="HGU4547803.1"
                     /translation="MKIGVFVPIGNNGWLISTHAPQYMPTFELNKAIVQKAEHYHFDF
                     ALSMIKLRGFGGKTEFWDHNLESFTLMAGLAAVTSRIQIYATAATLTLPPAIVARMAA
                     TIDSISGGRFGVNLVTGWQKPEYEQMGIWPGDDYFSRRYDYLTEYVQVLRDLWGTGKS
                     DFKGDFFTMNDCRVSPQPSVPMKVICAGQSDAGMAFSAQYADFNFCFGKGVNTPTAFA
                     PTAARMKQAAEQTGRDVGSYVLFMVIADETDDAARAKWEHYKAGADEEALSWLTEQSQ
                     KDTRSGTDTNVRQMADPTSAVNINMGTLVGSYASVARMLDEVASVPGAEGVLLTFDDF
                     LSGIETFGERIQPLMQCRAHLPALTQEVA"
     gene            13621..14313
                     /gene="rutB"
                     /locus_tag="ACHOSC_RS09265"
                     /old_locus_tag="pgaptmp_001853"
     CDS             13621..14313
                     /gene="rutB"
                     /locus_tag="ACHOSC_RS09265"
                     /old_locus_tag="pgaptmp_001853"
                     /EC_number="3.5.1.110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415531.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxyureidoacrylate/ureidoacrylate
                     amidohydrolase RutB"
                     /protein_id="HGU4547804.1"
                     /translation="MTTLTARPEAITFDPQQSALIVVDMQNAYATPGGYLDLAGFDVS
                     TTRPVIANIQTAVTAARAAGMLIIWFQNGWDEQYVEAGGPGSPNFHKSNALKTMRKQP
                     QLQGKLLAKGSWDYQLVDELVPQPGDIVLPKPRYSGFFNTPLDSILRSRGIRHLVFTG
                     IATNVCVESTLRDGFFLEYFGVVLEDATHQAGPEFAQKAALFNIETFFGWVSDVETFC
                     DALSPTSFARIA"
     gene            14325..14711
                     /gene="rutC"
                     /locus_tag="ACHOSC_RS09270"
                     /old_locus_tag="pgaptmp_001854"
     CDS             14325..14711
                     /gene="rutC"
                     /locus_tag="ACHOSC_RS09270"
                     /old_locus_tag="pgaptmp_001854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-aminoacrylate deaminase"
                     /protein_id="HGU4547805.1"
                     /translation="MPKSVIIPAGSSAPLAPFVPGTLADGVVYVSGTLAFDQHNNVLF
                     ADDPKAQTRHVLEIIRKVIETAGGTMADVTFNSIFITDWKNYAAINEIYAEFFPGDKP
                     ARFCIQCGLVKPDALVEIATIAHIAK"
     gene            14719..15519
                     /gene="rutD"
                     /locus_tag="ACHOSC_RS09275"
                     /old_locus_tag="pgaptmp_001855"
     CDS             14719..15519
                     /gene="rutD"
                     /locus_tag="ACHOSC_RS09275"
                     /old_locus_tag="pgaptmp_001855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016811 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds, in linear amides
                     [Evidence IEA];
                     GO_process: GO:0006212 - uracil catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine utilization protein D"
                     /protein_id="HGU4547806.1"
                     /translation="MKLSLSPPPYADTPVVVLISGLGGSGSYWLPQLAVLEQEYQVVC
                     YDQRGTGNNPDTLAEDYSIAQMAAELHQALVAAGIEHYAVVGHALGALVGMQLALDYP
                     ASVTVLISVNGWLRINAHTRRCFQVRERLLYSGGAQAWVEAQPLFLYPADWMAARAPR
                     LEAEDALALAHFQGKNNLLRRLNALKRADFSHHADRIRCPVQIICASDDLLVPSACSS
                     ELHAALPDSQKMVMPYGGHACNVTDPETFNALLLNGLASLLHHREAAL"
     gene            15529..16119
                     /gene="rutE"
                     /locus_tag="ACHOSC_RS09280"
                     /old_locus_tag="pgaptmp_001856"
     CDS             15529..16119
                     /gene="rutE"
                     /locus_tag="ACHOSC_RS09280"
                     /old_locus_tag="pgaptmp_001856"
                     /EC_number="1.1.1.298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malonic semialdehyde reductase"
                     /protein_id="HGU4547807.1"
                     /translation="MNEAVSPGALSTLFTDARTHNGWRETPVSDETLREIYALMKWGP
                     TSANCSPARIVFIRTAEGKERLRPALSSGNLQKTLTAPVTAIVAWDSEFYERLPQLFP
                     HGDARSWFTSSPQLAEETAFRNSSMQAAYLIVACRALGLDTGPMSGFDRQHVDDAFFA
                     GSTLKSNLLINIGYGDSSKLFARLPRLSFEEACGLL"
     gene            16130..16624
                     /gene="rutF"
                     /locus_tag="ACHOSC_RS09285"
                     /old_locus_tag="pgaptmp_001857"
     CDS             16130..16624
                     /gene="rutF"
                     /locus_tag="ACHOSC_RS09285"
                     /old_locus_tag="pgaptmp_001857"
                     /EC_number="1.5.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309280.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008752 - FMN reductase (NAD(P)H) activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-dependent FMN reductase RutF"
                     /protein_id="HGU4547808.1"
                     /translation="MNIVDQQTFRDAMSCMGAAVNIITTDGPAGRAGFTASAVCSVTD
                     TPPTLLVCLNRGASVWPVFNENRTLCVNTLSAGQEPLSNLFGGKTPMEHRFAAARWQT
                     GVTGCPQLEEALVSFDCRISQVVSVGTHDILFCAIEAIHRHATPYGLVWFDRSYHALM
                     RPAC"
     gene            16645..17973
                     /gene="rutG"
                     /locus_tag="ACHOSC_RS09290"
                     /old_locus_tag="pgaptmp_001858"
     CDS             16645..17973
                     /gene="rutG"
                     /locus_tag="ACHOSC_RS09290"
                     /old_locus_tag="pgaptmp_001858"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415526.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015218 - pyrimidine nucleotide
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0006864 - pyrimidine nucleotide transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine utilization transport protein G"
                     /protein_id="HGU4547809.1"
                     /translation="MAMFGFPHWQLKSTSTESGVVAPYERLPFAQTAVMGVQHAVAMF
                     GATVLMPILMGLDPNLSILMSGIGTLLFFFITGGRVPSYLGSSAAFVGVVIAATGFNG
                     QGINPNISIALGGIIACGLVYTVIGLVVMKIGTRWIERLMPPVVTGAVVMAIGLNLAP
                     IAVKSVSASAFDSWMAVMTVLCIGLVAVFTRGMIQRLLILVGLIVACLLYGVMTNLLG
                     LGKAVDFTLVSHAAWFGLPHFSTPAFNSQAMMLIAPVAVILVAENLGHLKAVAGMTGR
                     NMDPYMGRAFVGDGLATMLSGSVGGSGVTTYAENIGVMAVTKVYSTLVFVAAAVIAML
                     LGFSPKFGALIHTIPAAVIGGASIVVFGLIAVAGARIWVQNRVDLSQNGNLIMVAVTL
                     VLGAGDFALTLGGFTLGGIGTATFGAILLNALLSRKLVDVPPPEVVHQEP"
     gene            complement(18056..18229)
                     /gene="ymdF"
                     /locus_tag="ACHOSC_RS09295"
                     /old_locus_tag="pgaptmp_001859"
     CDS             complement(18056..18229)
                     /gene="ymdF"
                     /locus_tag="ACHOSC_RS09295"
                     /old_locus_tag="pgaptmp_001859"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415525.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006950 - response to stress [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="general stress protein"
                     /protein_id="HGU4547810.1"
                     /translation="MANHRGGSGNFAEDRERASEAGKKGGQHSGGNFKNDPQRASEAG
                     KKGGKSSHGKSDN"
     gene            18602..19198
                     /gene="wrbA"
                     /locus_tag="ACHOSC_RS09300"
                     /old_locus_tag="pgaptmp_001860"
     CDS             18602..19198
                     /gene="wrbA"
                     /locus_tag="ACHOSC_RS09300"
                     /old_locus_tag="pgaptmp_001860"
                     /EC_number="1.6.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003955 - NAD(P)H dehydrogenase (quinone)
                     activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H:quinone oxidoreductase"
                     /protein_id="HGU4547811.1"
                     /translation="MAKVLVLYYSMYGHIETMARAVAEGASKVDGAEVVVKRVPETMP
                     PQLFEKAGGKTQTAPVATPQELADYDAIIFGTPTRFGNMSGQMRTFLDQTGGLWASGA
                     LYGKLASVFSSTGTGSGQEQTITSTWTTLAHHGMVIVPIGYAAQELFDVSQVRGGTPY
                     GATTIAGGDGSRQPSQEELSIARYQGEYVAGLAVKLNG"
     gene            19219..19446
                     /gene="yccJ"
                     /locus_tag="ACHOSC_RS09305"
                     /old_locus_tag="pgaptmp_001861"
     CDS             19219..19446
                     /gene="yccJ"
                     /locus_tag="ACHOSC_RS09305"
                     /old_locus_tag="pgaptmp_001861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002898701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YccJ family protein"
                     /protein_id="HGU4547812.1"
                     /translation="MPTQEAKAHHVGEWASLRNTSPEIAEAIFEVAGYDEKMAEKIWE
                     EGSDEVLVKAFAKTDKDSLFWGEQTIERKNV"
     gene            complement(19484..20725)
                     /gene="agp"
                     /locus_tag="ACHOSC_RS09310"
                     /old_locus_tag="pgaptmp_001862"
     CDS             complement(19484..20725)
                     /gene="agp"
                     /locus_tag="ACHOSC_RS09310"
                     /old_locus_tag="pgaptmp_001862"
                     /EC_number="3.1.3.10"
                     /EC_number="3.1.3.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional glucose-1-phosphatase/inositol
                     phosphatase"
                     /protein_id="HGU4547813.1"
                     /translation="MNKTLIAATVAGIVLLASNAQAQTVPEGYQLQQVLMMSRHNLRA
                     PLANNGSVLEQSTPNKWPEWDVPGGQLTTKGGVLEVYMGHYMREWLAQQGMVKSGECP
                     PPDTVYAYANSLQRTVATAQFFITGAFPGCDIPVHHQEKMGTMDPTFNPVITDDSAAF
                     SEQAVAAMEKELSKLQLTDSYQLLEKIVNYKDSPACKEKQQCSLVDGKNTFSAKYQQE
                     PGVSGPLKVGNSLVDAFTLQYYEGFPMDQVAWGEIKSDQQWKVLSKLKNGYQDSLFTS
                     PEVARNVAKPLVSYIDKALVTDRTSAPKITVLVGHDSNIASLLTALDFKPYQLHDQNE
                     RTPIGGKIVFQRWHDSKANRDLMKIEYVYQSAEQLRNADALTLQAPAQRVTLELSGCP
                     IDANGFCPMDKFDSVLNEAVK"
     gene            complement(21017..>21270)
                     /locus_tag="ACHOSC_RS09315"
                     /old_locus_tag="pgaptmp_001863"
     CDS             complement(21017..>21270)
                     /locus_tag="ACHOSC_RS09315"
                     /old_locus_tag="pgaptmp_001863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000097604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4547814.1"
                     /translation="VKNFLTALSDSICSLFIVNGNVASISLGNESFSTDDDLEYGYLI
                     NTGNHYDVYLPPELFAQAYELNNKERNAQIDFLTRYAIY"
BASE COUNT         5006 a         5725 c         5510 g         5029 t
ORIGIN      
        1 tatagcccat tgttgattaa acctcataag caaaattggc accagatctc ctgtacaggt
       61 tgaatggccg aaaaaagata ggcagatgct catttttttg gcttctggaa gaaaaactct
      121 ccgcgctggc agtattacac tggtctgatg ctatgacgaa aaatgcctgg taagagtatc
      181 taaaccagac tccagttcag cttatcaaca cgctcaaagt cgcccccctg aacgctaacc
      241 tgaagtgaaa atctgttgaa ataaaacaaa gcccggttcg cccgggctct gcaccgataa
      301 tacacttaac tgtaggcatg cagcgtacgt tggcaaagtg ccgaacgtac gcagtcctct
      361 ttaccgaacc ggacgatccc aaccatttca tcttcttcga aacgttccag cgcgtcactt
      421 aatccggagc acacgccgcg aggcaaatcg cattgcgtga tatcaccgtt gacgataacc
      481 gtcacgttct ccccgaggcg ggttaaaaac attttcattt gcgcggcagt cacattctgc
      541 gcctcgtcaa gaatgacgac tgcattttca aaggtacgtc cacgcatata ggcgaacggc
      601 gcaatttcca ccttccctat ttccggtcgc aggcagtact gcataaagga agcccctaag
      661 cgccggacca gtacgtcgta gaccgggcga aaatagggag caaacttttc tgcgatatct
      721 ccaggtaaga agccaagatc ttcatcggct tgcagaactg gacgggtgac gataatcctg
      781 tcgacatcct tatgtatcag ggcttctgcc gcttttgctg cgctgatcca ggtttttccg
      841 cacccggctt cgcccgtggc gaatatcagc tgcttactct caatagcctt cagatagtgc
      901 aattgcgctt catttcgcgc gaggatgggc gaagtatcgc gactgtcgcg ggccatacca
      961 atggcttcta cgccgcccat ctgcacaagc gaggtgaccg attcttcttc acgctgctta
     1021 tggctgcgcg aatcccgtct cagcacacgt tttgcctcgc gacgagcttt gatcactgct
     1081 ttttgtcttc ccatggagag caccttgagt tgtttgtatt catcacacgc gccgttggca
     1141 gcgcgattat gcgcacgaac atcagagggt tggcttcctt gtaagccata gtttgctttt
     1201 ggataaaatg ccgaaaaacg gctacgcaca ccgtttgcgg cgtcggtaac acgagtaaag
     1261 agaggaagtg agaaaaatac agagtgacga agttgctacc ggaggagaaa tctccgcaag
     1321 tggtggagcg tagcttaaca agacattgtc cagaacagga cgttaccatc cgcgatctcc
     1381 atagtgactg actatcactg ccgggaactt ccgctgctac ttaataatta caacagatct
     1441 cgcattcatt gcaacaatat attcacttat atttaaccat aaaacaccat ttcagtgaca
     1501 ttagtttcta ctggaaagat gacagagtga tgacagatat gaaaaaagct gtgtgctttc
     1561 agcaggattc gcatcaggca tctgcgcacg aacgccggat gcggcgtgaa cgccttatcc
     1621 gcctaccgaa cccgcaccga ttgtaggcat aagaagacgc gcaagcatcg catcaggcat
     1681 ctgcgcaaga acgccttatc cgtctataaa cattaaaccc gcaataacgc gcttccgaga
     1741 taatcgttcg cgtccttcac tcccggcagc gcaaaaaaat aaccgccgcc gataggttta
     1801 acgtattcct ccagcgcttc gccattgagc cttttttgta ctgtcaggaa gcctttttcc
     1861 agatcgtgtt ggtaacagac aaacagtagc cccatatcca gttgcccgga gttggtgacg
     1921 cccagtgaat aactgtagcc acgacgcagc atcaggctgg actcactctc cgccgtgcgg
     1981 ggattcgcca gccggatatg gctgtccagc gcaatcacct tcccttccgg atcgctggcg
     2041 taatcgggaa catcatgctc atgctgcatt cccagcggcg caccggtttg cttatcacga
     2101 ccaaaaatcg tctgctgttc tttcagcgga gttctgtccc aaaattccac tcgaaactga
     2161 atcaagcgta ctgcctgata gctgccaccg attgtccacg ctggctcctg ctgatctgcc
     2221 gttacccaca ccactttttg catcaactta tcattctggc tatcgggatt ggcagtgccg
     2281 tctttgaaac ccagcaaatt aatcggcgtc tctttgcctt tactacgcgc cgcgtgatcg
     2341 gaaataaacc cttcccgctt ccagcgcacg ctgagcaaat ccggcgtatg tttgatgata
     2401 tcgcgcagtg catggatcac cgtgtcctgg gtgttggcgc aaatttgtag caacacatca
     2461 ccatgacata acgccgcatc cagcgagtcg ttgggaaaac gcgtcatctt ctgcaacttt
     2521 ttcggcatct gtggcgcaag gccaaagcgc tcatcaaaca atgagtgacc caccgataac
     2581 gtgatggtga gattatcggg cgcaatgtag ccgccaagaa tgccggaatc gagtggtggc
     2641 aggcgcggat ttggcgtttc tggtgccgct ccgccctgag tcagaaaagc aaaacgctga
     2701 gtcaacaagc gaaacaaccg ttcaagatcg gctttatcgc tggcaagcac atcaaacgcc
     2761 accagcatca ttgcggcctg ttgtggcgtc aggatccctg cctgatgctc accataaaac
     2821 ggctgtttct cattgcgtgc atccggtgaa agcgtacccg gcgcactttg cgttttttgt
     2881 gcatgagcga ccggacaact tcccgccagc gccagcgcac caatgccttt cagtaaacgt
     2941 cggcgtgacg gttcattcac gccgttttta tcttcatact gcataatgct taatccaatc
     3001 ccagcacacc gcgaagttgc gccagatctt ccgccagcgc agtaatcggt cctttcagtg
     3061 cattccggtc ggcatcggtc aatttgtcgt aggtttcaaa accgtcttta gtacggtatt
     3121 tcgccagaat ggtatcgacc tttttaaagt tggcatcgac ttttgccaac agttccgggt
     3181 tggctttttg cagttgtgga cgcagcagat cgacaatttt ctgcgagcct tcaacgttag
     3241 cctggaaatc ccacagatcg gtgtggctgt agcgatcttc ttcaccgctg attttgctgg
     3301 ctgccacttc ctcaatcagt ccggctgcac cgccgaccac ttttgaaggt gggaaagcca
     3361 gttcactgat gcgtttttgc aaatcgacca catcggtata aagctggtca gcgtactgat
     3421 ccatgccttt ggtggtgttg tcgccaaaca atgctttttc cagacggtgg aaaccggtga
     3481 attttggatc agcggctttt tgctcgtaat catcttcacg ggcgtcaatg ctgccatcca
     3541 gatcggagaa cagttcagca atcggttcaa tgcgctcata gtgctggcgc gtcggcgcat
     3601 acagtgcttt cgctttttcg atatcgcctg ctttaatcgc gtcggtaaag gctttggtgt
     3661 cggtcaccag ctgcgtggtt tccgccatga catacgcttt atatgcagta attgcaccac
     3721 caagacttaa cagcgcatca ctttgcgccg catccgccgt tgcctcacct ttgacgatca
     3781 acttcccttt cgggttagtc agcagaccgc aggtcatatc gtattcgcca ggctgtaaat
     3841 tcgccgtcat tttctggcta aaaccagggg cgatattttc ccgctcttcc accaccatca
     3901 cgcctttgag gatctcccac tccagcgcct tctggctatg gttctgaata atgaactgtg
     3961 ttttcccggc gttaaccgta atggtcatcg gttcgcactg cttatccgtc acggtcactt
     4021 tgacctgcgt cacatcagcg gcgttagcca taaaagcaga agaaaacagc gcagccacgc
     4081 tcaactgcaa tgcgttacgg cggaagttaa tggtcatgac gagactatcc ctttaaagta
     4141 tgttgtaact aagcaagagt ttgcgtcgta tttgttacgc ggagcgagac gctgtcgccc
     4201 ctgcgcgtgg tggcagagca aatgccacca gcgccgggat gagataaata aaccagacgg
     4261 cgacttcgct gacgctcggc gcttcctgat agccaaaaat cccttccatc agcgtgccaa
     4321 acagcgagtg agttgagagc accgcactca tatcgaaggc gatttcctga aagtggttcc
     4381 acaatccggc ttcatgaaat gcgcgaatgg caccagctgc cagccctgcg gcgacgaaga
     4441 gaataaacag gctggtccat ttaaaaaatg caccaagatt gaggcgaata ccgccccagt
     4501 agagcaagaa gcctagcacc acggcagtag caagaccgag cattgcaccc agcggcggcc
     4561 agatcccgac atcttgttga aatgccgcca gcaggaaaaa gaccgactcc agcccttccc
     4621 ttgcaacggc aaaaaagacc atcatcacca gcgcccagcc atgatgattt ccacgctgca
     4681 atgcgctatc gactgcctgc tccagttgta ctttgacgtt gcgcgacact ttgcgcatcc
     4741 agaaaaccat ccaggtaagg atcaccacgg cgatcaccgc cacgatacct tcaaacagtt
     4801 cctgctcttt ttgcggaaat tcgccggtgg tttcgttaat gaagatcccc aagcccaggc
     4861 acaacgcagc ggcaagcaac acgccaatcc acatcacacc aatccatcgg cctcgctggg
     4921 tacgcttaag atagctggca atcaaactga caatcagcgc ggcttcaagt ccttcgcgca
     4981 acataatgag aaacggaaca aacatgccac ccatccttaa acgtttttct gagtcaatct
     5041 atgcaaagaa aagtaaatcg cagtgatagc gattatcatt acggtaaaga aaaatacaag
     5101 cggaatgttt ataaaatgat gttgaaatgt aaacaaagag aggtaatgcc aggggatata
     5161 aacgattatt ccaggtacaa agatgggatt gtttacaacg agccatgtgt tcttgccgga
     5221 tgccgcgtaa acgccgtatc cagccgacaa aaccgtcctg actcaataca tcaaaacttc
     5281 catgtaaaac tgataagcgg gtgtatcaag caatttcgtc catcctcgat tatcaaatct
     5341 aactttttgc ccggaacata agaacagcgc gggtgttggt ggcgtcaaaa tcgctgaaac
     5401 gttccccgaa ggccggggca gcccacatgg atgtgggctg agggcgcgtc ttacaggacg
     5461 ttacctcgcg cccgacccgg tagccgaagg ggataagctg aaggtaccac gcagcggcga
     5521 ttttgttcgc cagagcccgg gggtgcaggg ggccgcggcg attggccgcc ccctgcgcgc
     5581 tccctgcacc attcaattta tatggcgagg cacattaagg gagcgcaacc cgctgctgaa
     5641 gccaaacaaa accgccgcag gctaagtccc ttagctttcc tgcaaccgtg acggcggagc
     5701 cgaatgatag tgcgcatcgg cctcggcaaa gcgtttttgc atcgccgctg acggcgcttt
     5761 acccagcaaa ctaaacacta caatcccgat actgccgaag atgaagcccg gaataatttc
     5821 gtagagtccc agccagccga actgcttcca gacgataacc gtcagcgcac cgatgatcat
     5881 ccccgccagc gcaccgttgc gcgtcatgcg tgaccacatc accgagaaca gcaccaccgg
     5941 accaaacgcc gcgccaaagc ctgcccacgc gtagctcact aagcccagca cgcggttttc
     6001 cgggtttgcc gccagcgcaa tcgccaccag cgccaccacc agcaccatca cacgtcctac
     6061 ccacaccagc tctttctggc tggcctgttt acgcagaaac gctttgtata aatcttcggt
     6121 aattgcactg gaacatacca gcagctggca acttaaggtt gacattaccg ccgccagaat
     6181 cgccgacagc agaatcccgg caatccacgg gttaaacaga atttgcgcca gttcgataaa
     6241 cacgcgctcg gcgttctggt ttaccgcgcc tgctaccgcc gggtgctcat taaagtaagc
     6301 aatcccaaag aagccaacag ccaccgcccc tgccaggcag aggatcatcc aggtcatact
     6361 gatacgacgc gcatggacaa tgctgtggtg agagtctgcc gccataaaac gcgccaggat
     6421 gtgcggctgc ccgaagtaac ccagccccca acccatcagt gagataatgg cgacaaagtt
     6481 cagccctttg agcatatcaa cgttttcgat gctcttttgt ttgatcactt ccagcgagtc
     6541 accaaagcca ccgacactga taatgacgat aaccggcgtc aggatcaggg caaaaatcat
     6601 caggctggcc tgtacagtgt cagtccagct caccgcgagg aaaccgccaa taaaggtgta
     6661 aaggatcgtc gccgcagctc ccgcccacag agccgtttcg tagctcatgc caaaggtact
     6721 ttcaaacaga cgcgcgcctg ccacaatgcc cgaagcgcaa taaatggtga agaacagcaa
     6781 aatcaccagc gcggagataa tgcgcaaaat gcggctttta tcttcaaagc gcccggtgaa
     6841 ataatccggc aatgttaagg cgttattgtt gtattcggta tgcacacgca accgccccgc
     6901 caccagcttc cagttgatcc acgcgcctaa tgtcaggcca atggcgatcc aactttcgga
     6961 aatcccggaa agaaaaaccg cgcccggcaa ccccattaac agccagccgc tcatatccga
     7021 cgcacccgcc gataatgccg tcacgaatgg cccaaggcta cgaccgccca gaatatagtc
     7081 gtcaaagttt ttcgttgatc gccaggcgat aaacccaatc aatatcatgc caaagatata
     7141 gacacaaaat gtcaccaaca tcggtgtgct aatagccatc taaagtctcc aaaaaattat
     7201 tatcggcaat gtcgaaactt gccgttatat ctgccaccgg aacggggtaa cagagtttat
     7261 gttttaccag ggcgaccgta tcctgccgga agcgctggtt attcacaatc gatttaacac
     7321 accatttaca ttaaatttta gtgctcagcg acactatttt tcatcaggtt gcactctctc
     7381 acattttttg cggttgcacc tttcaaaaat gttaactgcc gcagagaaaa agtctgagtt
     7441 atttttttaa tccctgtcat atcgatttct tttattaaca tttcattcat ttttaagctt
     7501 gctacgcatg tcacatttaa catggttgca caaagttgca acatcatgga tatttcacga
     7561 taacgttaag ttgcaccttt cagaacaaca ggagtaatgg catgggaacc accaccatgg
     7621 gggttaagct ggacgacgcg acgcgtgagc gtattaagtc tgccgcgaca cgtatcgatc
     7681 gcacgccaca ctggttaatt aagcaggcga ttttttctta tctcgaacaa ctggaaaaca
     7741 gcgatactct gccggagcta cctgcgctgc tttctggcgc tgccaatgag agcgatgaag
     7801 caccgactcc ggcagaggaa ccacaccaac cattcctcga ctttgccgag caaattttgc
     7861 ccctgtcggt ttcccgcgcc gcgatcacag cggcctatcg ccgcccggaa accgaagcgg
     7921 tttctatgct gctggaacaa gcccgcctgc cgcagccagt tgctgaacag gcgcacaaac
     7981 tggcgtatca actggccgat aaactgcgta atcaaaaaaa cgccagtggt cgcgcaggta
     8041 tggttcaggg gttattgcag gagttttcgt tgtcatcgca ggaaggcgtg gcgctgatgt
     8101 gtctggcgga agcgttgttg cgtattcccg acaaagccac ccgtgatgcg ttaattcgcg
     8161 acaaaatcag caacggtaac tggcaatcac acattggtcg tagcccgtct ttgtttgtta
     8221 atgccgccac ctgggggctg ctgtttactg gcaaactggt ttccacccat aacgaagcca
     8281 gcctctcccg ctcgctgaac cgcattatcg gtaaaagcgg tgaaccgctg atccgcaaag
     8341 gtgtggatat ggcgatgcgc ctgatgggcg agcagttcgt cactggcgaa accatcgcgg
     8401 aagcgttagc caatgcccgc aagctggaag agaaaggttt ccgttactct tacgatatgc
     8461 ttggcgaagc tgcactgact gccgcagatg cacaagcgta tatggtttcc tatcagcagg
     8521 cgattcacgc cattggtaaa gcgtccaatg gtcgtggcat ctatgaaggt ccgggcattt
     8581 caatcaagct gtcggcgctg catccgcgtt acagccgcgc ccaatatgac cgggtaatgg
     8641 aagagcttta cccacgtctg aaatcactca ccctgctggc gcgtcagtac gatattggta
     8701 tcaacattga cgccgaagag gccgatcgtc tggagatctc cctcgatctg ctggaaaaac
     8761 tctgtttcga gcctgagctg gcaggctgga acggtatcgg ttttgttatt caggcttatc
     8821 aaaaacgctg cccactggtg attgattacc tgattgatct cgccacccgc agccgtcgcc
     8881 gtctgatgat tcgcctggtg aaaggcgcgt actgggatag tgaaatcaag cgtgcgcaga
     8941 tggacggcct tgaaggctat ccggtttata cccgcaaggt gtataccgac gtttcatatc
     9001 tcgcctgtgc gaaaaagctg ctggcggtgc cgaatctaat ctacccgcag ttcgcgacgc
     9061 acaacgccca tacgctggca gcgatttatc aactggcagg tcagaactac tatccgggtc
     9121 agtacgagtt ccagtgcctg catggtatgg gcgagccact gtatgagcag gtcaccggga
     9181 aagttgccga cggcaaactt aaccgtccgt gtcgtattta tgctccggtc ggtacccatg
     9241 aaacgctgtt ggcgtatctg gtgcgtcgcc tgctggaaaa cggtgctaac acctcgtttg
     9301 ttaaccgtat tgccgacacc tctttgccac tggatgaact ggtcgccgat ccggtcactg
     9361 ctgtagaaaa actggcgcaa caggaagggc aaactggatt accgcatccg aaaattcccc
     9421 tgccgcgcga tctttacggt cacgggcgcg acaactcggc agggctggat ctcgctaacg
     9481 aacaccggct ggcctcgctc tcttctgccc tgctcaatag tgcactgcaa aaatggcagg
     9541 ccttgccaat gctggaacaa ccggtagcgg caggtgagat gtcgcccgtt attaaccctg
     9601 cggaaccgaa agatattgtg ggctttgtgc gtgaagccac gccgcgtgag gtagaacagg
     9661 cgctggaaag tgcggttaat aacgcgccaa tctggtttgc cactcctccg gttgaacgcg
     9721 cggcgatttt gcatcgcgct gccgtgctaa tggaaagcca gatgcagcaa ctgattggta
     9781 ttctggtgcg tgaagccgga aaaaccttca gtaacgccat tgccgaagtg cgcgaagcgg
     9841 tcgattttct ccactactat gccggacagg tgcgggatga tttcgctaac gaaacccacc
     9901 gtccattagg gcctgtggtg tgtatcagtc cgtggaactt cccgctggct attttcaccg
     9961 ggcagattgc tgccgcactg gcggcaggta acagcgtgct ggcaaaaccg gcagaacaaa
    10021 cgccgctgat tgccgcgcaa gggatcgcca ttttgctgga agcgggtgta ccgccaggcg
    10081 tggtgcagtt gctgccaggt cagggggaaa ccgtgggcgc gcaactgacg ggtgatgatc
    10141 gcgtacgcgg ggtgatgttt accggttcaa ccgaagtcgc tacgttactg cagcgcaata
    10201 tcgccagccg cctggacgct cagggtcgcc ctattccgct catcgctgaa accggcggca
    10261 tgaacgcgat gattgtcgat tcttcagcac tgaccgaaca ggtcgtcgtg gatgtactgg
    10321 cctcggcgtt cgacagtgcg ggtcagcgtt gttcggcgct gcgcgtgctg tgcctgcaag
    10381 atgagattgc cgaccacacg ttgaaaatgc tgcgcggcgc aatggccgaa tgccggatgg
    10441 gtaatccggg tcgcctgacc accgatatcg gtccggtgat tgatagcgaa gcgaaagcca
    10501 atattgagcg ccatattcag accatgcgta gcaaaggccg tccggtgttc caggcggtgc
    10561 gggaaaacag cgaagatgcc cgtgaatggc aaagcggcac ctttgtcgcc ccgacgctga
    10621 tcgaactgga tgactttgcc gaattgcaaa aagaggtctt tggtccggtg ctgcatgtgg
    10681 tgcgttacaa ccgtaaccag ctaccagagc tgatcgagca gattaacgct tccggctatg
    10741 gtctgacgct tggtgtccat acgcgtattg atgaaaccat cgcccaggtc actggctcgg
    10801 cccatgttgg taacctgtat gttaaccgta atatggtggg cgcagtggtg ggtgtgcagc
    10861 cattcggcgg cgaagggttg tccggtaccg ggccgaaagc aggcggtccg ctctatctct
    10921 accgtctgct ggcgaatcgc ccggaaagtg cgctggcagt gacgctcgcg cgtcaggatg
    10981 cagagtatcc ggtcgatgca cagttgaaag ccgcattgac tcagccgcta aatgcactgc
    11041 gggaatgggc agcaaatcgt ccagaattgc aggcgttatg tacgcaatat ggcgagctgg
    11101 cgcaggcagg aacacaacga ttgctgccgg ggccgacggg tgaacgcaac acctggacgc
    11161 tgctgccgcg tgagcgcgtg ttgtgtattg ccgatgatga gcaggatgcg ctgactcagc
    11221 tcgccgccgt gctggcggtg ggcagccagg tactgtggcc ggatgacgcg ctgcatcgtc
    11281 agctagtaaa ggcattgcca tcggcagtca gcgaacgtat tcaactggcg aaagcggaaa
    11341 atataaccgc tcaacccttt gatgcggtga tcttccacgg tgattcggat cagcttcgcg
    11401 cattgtgtga agccgttgct gcgcgggatg gcgcaattgt ttcggtgcag ggttttgccc
    11461 gtggcgaaag caatatcctt ctggaacggc tgtatatcga acgttcgctg agtgtgaata
    11521 ccgctgccgc tggcggtaac gccagtttaa tgactatagg ttaaacaggt agccggagga
    11581 tgttacaacc tcctccggca tctttaacgt ggtcgaatcc cctcaataat aatccgctgc
    11641 acgttttcaa ccgtttgatt gaaaaatatc tcatcgcgca acgtcgcgcc tgtcaccgcc
    11701 tccacctgag gggcgaaatc ggcgtaatgt tgagtggaag cccaaatcat gaaaatcaaa
    11761 tgctgcggat caatcggcgc gagtttgccg cttttgaccc aaccggcaat cagcgccgat
    11821 ttctcatcaa ttaatgcctt caaatcgccc gtcagttcat ccattaacag cggcgcgcct
    11881 gccagcatct ccatacagaa caggcgcgaa gcctgcggat aatcgcggga gacttccagc
    11941 ttcagacgga tgtactcttt tatcgccgcc agcggagcga aatcttcacg aaacgctttt
    12001 aacggtgcca gccagatatc gagaatctgc cgcagcacgg caatatacag cgcctctttt
    12061 gacggaaagt aatacagcag attggttttt gaaacacccg ccaactctgc gatctgctcc
    12121 agccttgtgc cgtgaaaacc gaattgtgaa aaagtgtcca gtgctgcgct aagaatcgct
    12181 tttttcttcg cgcttactgc gcgcgaacgt ttacccgttg ttttcactgc gccttgcgtc
    12241 atgcgctctc ccctcttgta gattggcaac aggatagcaa aacccccgcc cggcctcgac
    12301 cttctgcact ctcatcgcgc acagatgcat gttttatgtg caactgtttt gaccgtttag
    12361 tccacttttt accagatatt ttagacaaca caatgttaac acattaataa cattcacttt
    12421 ttaaaaactg gcatccgctt tgcaaacaag ccattacgcg gctgaaaagg atatgcagga
    12481 tgcagcgccc cgtctgactt tcactttacg agacgaagag aggttgatga tgaaaattgg
    12541 cgtattcgta cctattggca acaacggctg gctcatttcg acccacgcgc cgcagtacat
    12601 gccgaccttt gaactgaata aagccattgt gcaaaaagcg gagcactacc atttcgattt
    12661 cgccctgtcg atgatcaaac tgcgtggctt tggcggcaaa actgagttct gggatcacaa
    12721 ccttgagtcg ttcaccttga tggcggggct ggcggccgtc acctcgcgca ttcagattta
    12781 cgccactgct gccaccttaa cgttacctcc ggcaatcgtc gcccgtatgg ccgcaaccat
    12841 cgactccatc tctggcgggc gttttggcgt caacctcgtg actggctggc aaaagcccga
    12901 gtatgagcag atgggtatct ggcctggcga tgactatttc tcccgtcgtt acgactatct
    12961 caccgaatat gttcaggtgc tgcgcgacct gtggggcacg gggaaaagcg attttaaagg
    13021 cgattttttc accatgaatg attgtcgcgt cagtccgcaa ccgagtgtcc ccatgaaagt
    13081 gatctgcgcc gggcaaagcg acgctggcat ggcgttctcc gcccagtatg ccgatttcaa
    13141 cttctgtttc ggcaaaggcg taaatacgcc cacggctttc gccccgaccg ctgcgcggat
    13201 gaaacaggcc gcagagcaaa ccgggcgcga cgttggttct tatgtgttgt ttatggtgat
    13261 tgccgatgaa accgacgatg ccgctcgcgc caaatgggaa cactacaaag cgggcgcgga
    13321 tgaagaggcg ttaagctggc taaccgaaca aagtcagaaa gatacccgct ccggtactga
    13381 taccaacgtc cgtcagatgg ccgatcccac ttcggcggta aacatcaata tggggacgtt
    13441 agtcggttct tacgccagtg tcgcgcgcat gttagatgaa gtcgcaagcg tgcctggtgc
    13501 cgaaggcgtg ctgttgacct tcgatgattt tctgtcggga atcgaaacct tcggcgagcg
    13561 cattcaacca ctgatgcagt gccgcgccca tctacctgcg ctgactcagg aggtggcatg
    13621 atgacaacct taaccgctcg accggaagcc attaccttcg atccgcagca aagtgcgctg
    13681 atcgtggtgg atatgcaaaa cgcttatgcc acgccaggcg gctacttaga tctcgccggg
    13741 tttgatgtct caaccactcg cccggtcatt gccaacattc aaaccgccgt gaccgcagcg
    13801 cgagcggcag ggatgctgat catctggttt caaaatggct gggatgaaca gtatgtcgaa
    13861 gctggcggcc ccggctcacc gaattttcat aaatcgaacg ccctgaaaac catgcgtaag
    13921 cagccgcagc tgcaggggaa attgctggcg aaaggctcct gggattatca actggtggat
    13981 gaactggtgc cgcagcctgg cgatattgtg ctgccgaagc cgcgctacag cggtttcttc
    14041 aatacaccgc tggacagcat tttgcgcagc cgtggaatac gccatctggt tttcaccggt
    14101 atcgctacca acgtctgcgt cgaatcgacg ctacgcgatg gcttttttct ggagtatttc
    14161 ggcgtggtgc tggaagacgc aacacaccag gcggggccgg aatttgcaca gaaagccgcg
    14221 ttgttcaata tcgaaacctt ttttggctgg gtcagcgacg tcgaaacgtt ctgcgacgcg
    14281 ctttctccca catcctttgc tcgtatcgct taaggagttt aacgatgcca aaatccgtaa
    14341 ttattcccgc tggcagcagc gcaccgctgg cccccttcgt tcccggcacg ctggctgatg
    14401 gcgtggtgta tgtctccggt acgctggctt ttgatcaaca taataacgtg ctgtttgccg
    14461 atgacccaaa ggcgcaaacc cgccacgttc tggaaattat ccgcaaggtg atcgagacgg
    14521 cgggtggcac gatggcggat gtgaccttca acagcatctt tattaccgac tggaaaaatt
    14581 acgccgcgat taacgaaatc tacgccgagt tttttccggg tgataaaccg gcgcgattct
    14641 gcattcagtg cggactggta aaacctgacg cgctggtgga aatcgccaca attgcgcata
    14701 tcgccaagtg aggccgcgat gaaactttca ctctcacctc ccccttatgc tgatacgccc
    14761 gtagtggtgt tgatttcggg tcttggcggt agcggcagtt actggttacc gcaactggcg
    14821 gtgctggagc aggagtatca ggtagtctgt tacgaccagc gcggcaccgg caataatccc
    14881 gacacgctgg cagaagatta cagtatcgcc cagatggcag cggaactgca tcaggcgctg
    14941 gtagccgcag ggattgagca ttacgcggtg gtcggccatg cgctcggtgc gctggtggga
    15001 atgcagctgg cgctggatta tcccgcgtcg gtaactgtgc tgatcagcgt taacggctgg
    15061 ctacgaataa acgcccatac gcgccgctgt tttcaggttc gcgaacgatt actgtatagc
    15121 ggcggcgcgc aggcatgggt ggaagcgcag ccgttgttcc tctatcccgc cgactggatg
    15181 gcggcccgcg cacctcgcct ggaggcagaa gacgcgctgg cactggcgca ttttcagggc
    15241 aaaaataatt tactgcgtcg acttaacgcc ctcaaacgcg ctgactttag tcaccatgcg
    15301 gatcgcatcc gctgcccggt gcaaatcatc tgcgccagtg atgatctgct ggtgccatca
    15361 gcatgttcca gtgagcttca tgccgccctg cccgatagcc agaaaatggt gatgccctat
    15421 ggcggacacg cctgcaacgt gaccgatccc gaaacgttta atgctctgtt actcaacggg
    15481 cttgccagcc tgttacatca ccgtgaagcc gccctgtaag gaattgctat gaacgaagcc
    15541 gttagcccag gtgcgcttag caccttgttc accgatgccc gcactcacaa cggctggcgg
    15601 gagacacccg tcagcgatga gacgttacgg gagatttatg ccctgatgaa atgggggccg
    15661 acatcagcta actgttctcc ggcacggatc gtgtttatcc gcacggcaga aggaaaagaa
    15721 cgtctgcgcc cggcactttc cagcggcaat ctgcaaaaaa ccctgaccgc gcccgtcacc
    15781 gctatcgtcg cctgggacag tgaattttat gaacggttac cgcaactgtt tcctcacggc
    15841 gatgcccgca gttggtttac ctccagccca caacttgccg aagaaacagc gtttcgcaac
    15901 agttccatgc aggcggccta tctgatcgtc gcctgccgtg cgctgggact ggataccggc
    15961 ccgatgtcgg gttttgaccg tcaacacgtt gacgacgcct tttttgcggg cagcacgctg
    16021 aagagcaatc tgctgattaa tatcggctat ggcgatagca gcaaactttt tgcgcgcctg
    16081 ccacgtctgt cctttgaaga agcctgcggg ctgttgtaag gagcaaacca tgaacattgt
    16141 cgatcaacaa acctttcgcg atgcgatgtc ctgtatgggc gcggcagtca atatcatcac
    16201 cacggatggt ccagccgggc gcgccgggtt caccgccagc gcggtctgca gcgtgaccga
    16261 cacgccgcct acattactgg tgtgcctgaa tcgtggggcg tccgtctggc cggtattcaa
    16321 tgaaaatcga acgctgtgtg taaatacact gagcgccggg caggagccgc tttcaaatct
    16381 ttttggcggc aaaacgccca tggaacaccg ctttgccgcc gcccgctggc agacgggtgt
    16441 gaccggatgc ccgcaactgg aagaggctct ggtttcgttt gactgccgta tcagccaggt
    16501 ggtgagcgtc ggcacccacg acattctgtt ttgcgccatc gaagcgattc atcgtcacgc
    16561 cacaccctac gggctggtgt ggttcgatcg cagttatcac gcgctgatgc gccctgcttg
    16621 ttaacctcct aaaggagaca gctgatggca atgttcggtt ttcctcactg gcagttaaaa
    16681 tcgacctcta cagagagcgg cgtggtcgcg ccgtatgaac gattgccgtt tgctcagacg
    16741 gcagtaatgg gcgttcaaca cgcggtggcg atgtttggcg cgacggtgtt aatgccgatt
    16801 ctgatggggc tggatcccaa tctttccatt ttaatgtcgg ggatcggcac gttactgttt
    16861 ttctttatta ccggcgggcg cgtccccagt tatctcggtt ccagcgcggc ttttgtcggc
    16921 gtggtgattg ccgcgacggg ctttaacggt cagggcatta acccgaatat cagcattgcg
    16981 ctgggcggga ttatcgcctg tgggctggtt tataccgtca ttggcctggt ggtgatgaaa
    17041 attggtacac gctggattga acgactaatg ccgccggtgg tgacgggtgc ggtggtgatg
    17101 gcgattggcc tgaacttagc gcctatcgca gtgaaaagtg tatcggcttc ggcctttgat
    17161 agctggatgg cggtaatgac ggtgctgtgt attgggctgg tggcggtgtt tacccgtggc
    17221 atgatccagc ggttattgat tctcgtaggt ttaattgtcg cctgtctgct gtatggcgtg
    17281 atgaccaacc ttttagggct gggaaaagcc gtcgatttta ctctcgtcag ccatgctgcc
    17341 tggttcggtt tgccacattt ttccactcct gcttttaata gtcaggcaat gatgctgatt
    17401 gcgcccgtgg cggtgattct ggtggcggaa aacttaggtc atctcaaggc ggtcgccggg
    17461 atgaccggac gcaatatgga cccgtatatg gggcgggcat tcgtcggtga tgggctggcg
    17521 acgatgcttt ctggttctgt cggtggcagc ggcgtaacca cctacgccga gaatatcggt
    17581 gtaatggccg tgacaaaagt ctactcgacg ctggtgtttg tcgctgctgc cgtcatcgcc
    17641 atgctgttgg gcttttcacc gaaatttggc gcgctgattc ataccattcc ggccgcggtg
    17701 attggtggtg catcaattgt tgtgttcggg ctgattgccg tcgcaggcgc aaggatatgg
    17761 gtacaaaacc gtgtcgattt aagccagaac ggtaatttga ttatggtcgc agtaacctta
    17821 gtgctgggcg cgggtgattt tgctctcacg ctgggcggtt ttacgttggg agggattggt
    17881 acagcaacct ttggcgcgat tttactcaat gcgttgttaa gcagaaagtt ggttgacgtt
    17941 ccaccgccag aagttgttca tcaggagcca taaacagaaa accgccggga ttaccggcgg
    18001 tgatgacttt ttgacgagcc taagaaaaaa atgcattcgt cattgattag cccggctagt
    18061 tgtcgctttt gccgtgactg ctcttgccac cttttttacc tgcttcagat gcgcgctgcg
    18121 ggtcattctt gaaattaccc ccgctgtgct gtccaccttt tttacctgct tctgatgctc
    18181 tttcgcggtc ttctgcaaaa ttgccggaac cgcctcgatg gtttgccatt actgacctcc
    18241 gtctgggtat agacatcata ttgcatagaa ttaacgcggc atatggcggc tgccatattt
    18301 tcgctacgtg ggactaagct tagtgaactg acgcgggcag acccgcgttt ggtaatatcc
    18361 tgcaacagct tcttagcgag aatagatatc atctcaaata attatccata agccgctctt
    18421 atgattaaaa aaacgcgatg agataaaaat gtgtcatttt gcgacaaaat tacgtgcttg
    18481 tgaaagttgt tataaatcaa ataagtggtt gtgaaatttg cactctgaaa aggacgtctt
    18541 atctttaaat aagtggtagc gaatcgctac ggaatagaga taacacgagg agtggttaga
    18601 aatggctaaa gttctggtgc tttattattc catgtacgga catattgaaa cgatggcacg
    18661 cgcagtcgct gagggtgcaa gcaaagtcga tggcgcagaa gttgtcgtta agcgtgtacc
    18721 ggaaaccatg ccgccgcaat tatttgaaaa agcaggcggt aaaacgcaaa ctgcaccggt
    18781 tgcaaccccg caagaactgg ccgattacga cgccattatt tttggtacac ctacccgctt
    18841 tggcaacatg tccggtcaaa tgcgtacctt cctcgaccag acgggcggcc tgtgggcttc
    18901 cggcgcacta tacggaaaac tggcgagcgt ctttagttcc accggtactg gcagcggtca
    18961 ggaacaaact attacttcaa cctggacgac ccttgcgcat cacggcatgg taattgtccc
    19021 cattggctac gcagcgcagg aattatttga cgtttcacag gttcgcggcg gtacgccgta
    19081 cggcgcaacc accatcgcag gcggtgacgg ctcacgccag ccaagccagg aagaactgtc
    19141 tattgctcgt tatcaagggg aatatgtcgc aggtctggca gttaaactta acggctaatc
    19201 ttcaacagga ggatacgcat gccaactcaa gaagcgaaag ctcatcacgt cggtgaatgg
    19261 gcatctctgc gcaatacgtc gccggaaata gccgaggcca ttttcgaagt cgccgggtac
    19321 gacgaaaaaa tggcggaaaa gatttgggaa gaaggtagcg atgaagtgtt agtcaaagcc
    19381 tttgctaaga ccgataaaga ttcgcttttt tggggcgaac agaccatcga acgtaaaaac
    19441 gtttaaccag cgactccccc acttctcgcg ggggagtttt ctgttatttc accgcctcat
    19501 tcaacacgct atcaaactta tccattgggc agaaaccgtt ggcatctatc gggcaaccgc
    19561 ttaattccag cgtcacacgc tgagcaggtg cctgcagggt taacgcatcg gcattacgta
    19621 actgttccgc actctgatac acatattcaa ttttcatcaa atcgcgattg gctttgctgt
    19681 catgccaacg ctggaaaacg attttgccgc caatcggcgt gcgttcgttc tggtcatgca
    19741 actgatacgg tttgaaatcc agcgccgtta acagagaggc aatgttggag tcgtgcccaa
    19801 ccaacactgt aattttcggt gcgctggtgc gatcggtgac cagagctttg tcgatataac
    19861 tgaccagcgg tttcgcaaca ttgcgcgcca cttccggtga ggtaaacagg ctgtcctggt
    19921 agccgttttt aagcttcgac aacaccttcc actgctggtc agatttgatt tctccccagg
    19981 ccacctgatc catcggaaaa ccttcgtaat attgcaaagt aaacgcatct accagcgagt
    20041 tgccgacttt cagcggcccg gaaacacctg gttcttgttg atacttggcg ctaaaggtat
    20101 ttttgccatc caccagcgaa cactgttgtt tctctttaca ggcaggggaa tctttatagt
    20161 taacgatttt ttccagtagt tggtagctgt cggtaagctg gagtttgctg agctctttct
    20221 ccattgccgc caccgcctgt tcactgaatg cggcggaatc atcggtgatc accgggttaa
    20281 aggttgggtc catagtgccc attttttcct ggtgatgcac aggaatatca caccccggga
    20341 atgcgccggt aataaagaac tgtgcggtcg caacggtacg ttgcagacta ttggcatagg
    20401 cataaacggt gtccggcggc gggcattccc ccgatttcac catcccctgc tgtgccagcc
    20461 attcacgcat gtaatggccc atatacactt cgagcacgcc acctttggtg gtgagttgcc
    20521 caccggggac gtcccattcg ggccatttat tcggcgtaga ctgctccagc acactgccat
    20581 tgttcgccag cggcgcacgt aagttatggc ggctcatcat gagcacttgc tgtagctgat
    20641 agccttccgg tacggtttgt gcctgagcat ttgaagcgag taaaactatc cctgccacag
    20701 ttgcggcgat tagcgttttg ttcattcctg gcacctcttt tgttatcaat cactaaagag
    20761 tgtgacagaa atatgacatt tttccgggaa caggttcgca aaatggcttg ttgaatgcct
    20821 gtttttctat atacatcttt aaaaacaggc aaggtttgac gcaaactatg cctgattagc
    20881 tccctgcctc gcgtacgccg ctgatgatta cacctgtggc taaacagaat atcagcggcg
    20941 ttactaatac agaggcaata gtaccaataa taagagaagt gaggctaata acaatggttt
    21001 ttagcacatg cctttgtcag taaattgcat aacgagttaa aaaatcaatt tgcgcattcc
    21061 tttccttatt gtttaactcg taagcctgag caaaaagttc aggagggagg taaacgtcat
    21121 aatgattgcc agtgtttatt aaatacccat actcaaggtc gtcatctgtt gaaaaggatt
    21181 cattccctaa ggagattgat gccacattac cattaacaat aaataatgag caaatgctat
    21241 ctgaaagagc agtgaggaaa tttttcacct
//
LOCUS       DAZKHF020000062        21255 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2201S31241
            SAMN43267800-rid25106413.denovo.062, whole genome shotgun sequence.
ACCESSION   DAZKHF020000062 DAZKHF020000000
VERSION     DAZKHF020000062.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267800
            Sequence Read Archive: SRR30307263
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 21255)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21255)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 21255)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350581.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350581.2
            Genome Coverage       :: 151x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:21:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,652
            CDSs (total)                      :: 5,557
            Genes (coding)                    :: 5,320
            CDSs (with protein)               :: 5,320
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 237
            CDSs (without protein)            :: 237
            Pseudo Genes (ambiguous residues) :: 0 of 237
            Pseudo Genes (frameshifted)       :: 102 of 237
            Pseudo Genes (incomplete)         :: 130 of 237
            Pseudo Genes (internal stop)      :: 52 of 237
            Pseudo Genes (multiple problems)  :: 41 of 237
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21255
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267800-rid25106413.denovo.062"
                     /serotype="O 26:H11"
                     /isolate="2201S31241"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-01"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            <1..289
                     /locus_tag="ACHOSC_RS09320"
                     /old_locus_tag="pgaptmp_001864"
     CDS             <1..289
                     /locus_tag="ACHOSC_RS09320"
                     /old_locus_tag="pgaptmp_001864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HGU4547815.1"
                     /translation="SVSYICHEFTKEYRISPINYVIQRRMTEAKWSLTNTELSQAEIS
                     WRVGYENVDHFAKLFLRHVGCSPSDYRRQFKNCFAEQEILSEFPQPVSLVG"
     gene            complement(332..1483)
                     /gene="ydiO"
                     /locus_tag="ACHOSC_RS09325"
                     /old_locus_tag="pgaptmp_001865"
     CDS             complement(332..1483)
                     /gene="ydiO"
                     /locus_tag="ACHOSC_RS09325"
                     /old_locus_tag="pgaptmp_001865"
                     /EC_number="1.3.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310429.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase"
                     /protein_id="HGU4547816.1"
                     /translation="MDFSLTEEQELLLASIRELITTNFPEEYFRTCDQNGTYPREFMR
                     ALADNGISMLGVPEEFGGIPADYVTQMLALMEVSKCGAPAFLITNGQCIHSMRRFGSA
                     EQLRKTAESTLETGDPAYALALTEPGAGSDNNSATTTYTRKNGKVYINGQKTFITSAK
                     EYPYMLVLARDPQPKDPKKAFTLWWVDSSKPGIKINPLHKIGWHMLSTCEVYLDNVEV
                     EESDMVGEEGMGFLNVMYNFEMERLINAARSTGFAECAFEDAARYANQRIAFGKPIGH
                     NQMIQEKLALMAIKIDNMRNMVLKVAWQADQHQSLRTSAALAKLYCARTAMEVIDDAI
                     QIMGGLGYTDEARVSRFWRDVRCERIGGGTDEIMIYVAGRQILKDYQNK"
     gene            complement(1497..3091)
                     /gene="ydiF"
                     /locus_tag="ACHOSC_RS09330"
                     /old_locus_tag="pgaptmp_001866"
                     /pseudo
     CDS             complement(1497..3091)
                     /gene="ydiF"
                     /locus_tag="ACHOSC_RS09330"
                     /old_locus_tag="pgaptmp_001866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310428.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="acyl CoA:acetate/3-ketoacid CoA transferase"
     gene            complement(3235..3993)
                     /gene="aroD"
                     /locus_tag="ACHOSC_RS09335"
                     /old_locus_tag="pgaptmp_001867"
     CDS             complement(3235..3993)
                     /gene="aroD"
                     /locus_tag="ACHOSC_RS09335"
                     /old_locus_tag="pgaptmp_001867"
                     /EC_number="4.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003855 - 3-dehydroquinate dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I 3-dehydroquinate dehydratase"
                     /protein_id="HGU4547817.1"
                     /translation="MKTVTVKDLVIGTGAPKIIVSLMAKDIARVKSEALAYREADFDI
                     LEWRVDHFADLSNVESVMAAAKILRETMPEKPLLFTFRSAKEGGEQAISTEAYIALNR
                     AAIDSGLVDMIDLELFTGDDQVKETVAYAHAHDVKVVMSNHDFHKTPEAEEIIARLRK
                     MQSFDADIPKIALMPQSTSDVLTLLTATLEMQEQYADRPIITMSMAKTGVISRLAGEV
                     FGSAATFGAVKKASAPGQISVNDLRTVLTILHQA"
     gene            complement(4024..4890)
                     /gene="ydiB"
                     /locus_tag="ACHOSC_RS09340"
                     /old_locus_tag="pgaptmp_001868"
     CDS             complement(4024..4890)
                     /gene="ydiB"
                     /locus_tag="ACHOSC_RS09340"
                     /old_locus_tag="pgaptmp_001868"
                     /EC_number="1.1.1.282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinate/shikimate dehydrogenase"
                     /protein_id="HGU4547818.1"
                     /translation="MDVTAKYELIGLMAYPIRHSLSPEMQNKALEKAGLPFTYMAFEV
                     DNDSFPGAIEGLKALKMRGTGISMPNKQLACEYVDELTPAAKLVGAINTIVNDDGYLR
                     GYNTDGTGHIRAIKESGFDIKGKTMVLLGAGGASTAIGAQGAIEGLKEIKLFNRRDEF
                     FDKALAFAQRVNENTDCVVTVTDLADQQAFAEALASADILTNGTKVGMKPLENESLVN
                     DISLLHPGLLVTECVYNPHMTKLLQQAQQAGCKTIDGYGMLLWQGAEQFTLWTGKDFP
                     LEYVKQVMGFGA"
     gene            complement(4902..6167)
                     /gene="ydiN"
                     /locus_tag="ACHOSC_RS09345"
                     /old_locus_tag="pgaptmp_001869"
     CDS             complement(4902..6167)
                     /gene="ydiN"
                     /locus_tag="ACHOSC_RS09345"
                     /old_locus_tag="pgaptmp_001869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310425.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HGU4547819.1"
                     /translation="MSQNKAFSTPFILAVLCIYFSYFLHGISVITLAQNMSSLAEKFS
                     TDNAGIAYLISGIGLGRLISILFFGVISDKFGRRAVILMAVIMYLLFFFGIPACPNLT
                     LAYGLAVCVGIANSALDTGGYPALMECFPKASGSAVILVKAMVSFGQMFYPMLVSYML
                     LNNIWYGYGLIIPGILFVLITLMLLKSKFPSQLVDASVANELPQMNSKPLVWLEGVSS
                     VLFGVAAFSTFYVIVVWMPKYAMAFAGMSEAEALKTISYYSMGSLVCVFIFAALLKKM
                     VRPIWANVFNSALATITAAIIYLYPSPLVCNAGAFVIGFSAAGGILQLGVSVMSEFFP
                     KSKAKVTSIYMMMGGLANFVIPLITGYLSNIGLQYIIVLDFTFALLALITAIIVFIRY
                     YRVFIIPENDVRFGERKFSTRLNTIKHRG"
     gene            complement(6394..7608)
                     /gene="ydiM"
                     /locus_tag="ACHOSC_RS09350"
                     /old_locus_tag="pgaptmp_001870"
     CDS             complement(6394..7608)
                     /gene="ydiM"
                     /locus_tag="ACHOSC_RS09350"
                     /old_locus_tag="pgaptmp_001870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HGU4547820.1"
                     /translation="MKNPYYPTALGLYFNYLVHGMGVILMSLNMASLETLWQTNAAGV
                     SIVISSLGIGRLSVLLFAGLLSDRFGRRPFIMLGMCCYMAFFFGILHTNNIIIAYVFG
                     FLAGMANSFLDAGTYPSLMEAFPRSPGTANILIKAFVSSGQFLLPLIISLLVWAELWF
                     GWSFMIAAGIMFINALFLYRCTFPPHPGHRLPVIKKTTSSTEHRCSIIDLASYTLYGY
                     ISMATFYLVSQWLAQYGQFVAGMSYTMSIKLLSIYTVDSLLCVFITAPLIRNTVRPTT
                     LLMLYTFISFIALFTVCLHPTFYVVIIFAFVIGFTSAGGVVQIGLTLMAERFPYAKGK
                     ATGIYYSAGSIATFTIPLITAHLSQRSIADIMWFDTAIAAIGFLLALFIGLRSRKKTR
                     HHSLKENVAPGG"
     gene            complement(7708..8064)
                     /gene="ydiL"
                     /locus_tag="ACHOSC_RS09355"
                     /old_locus_tag="pgaptmp_001871"
     CDS             complement(7708..8064)
                     /gene="ydiL"
                     /locus_tag="ACHOSC_RS09355"
                     /old_locus_tag="pgaptmp_001871"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707588.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdiL family protein"
                     /protein_id="HGU4547821.1"
                     /translation="MNAYELQALRHIFAMTIDECATWIAQTGDSESWRQWENGKCAIP
                     DRVVEQLLAMRQQRKKHLHAIIEKINNRIGNNTMRFFPDLTAFQQVYPDGNFIDWKIY
                     QSVAAELYAHDLERLC"
     gene            complement(8200..8307)
                     /gene="rprA"
                     /locus_tag="ACHOSC_RS09360"
                     /old_locus_tag="pgaptmp_001872"
     ncRNA           complement(8200..8307)
                     /ncRNA_class="antisense_RNA"
                     /gene="rprA"
                     /locus_tag="ACHOSC_RS09360"
                     /old_locus_tag="pgaptmp_001872"
                     /product="antisense sRNA RprA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00034"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00034"
     gene            complement(8493..9605)
                     /gene="ydiK"
                     /locus_tag="ACHOSC_RS09365"
                     /old_locus_tag="pgaptmp_001873"
     CDS             complement(8493..9605)
                     /gene="ydiK"
                     /locus_tag="ACHOSC_RS09365"
                     /old_locus_tag="pgaptmp_001873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter YdiK"
                     /protein_id="HGU4547822.1"
                     /translation="MVNVRQPRDVAQILLSVLFLAIMIVACLWIVQPFILGFAWAGTV
                     VIATWPVLLRLQKIMFGRRSLAVLVMTLLLVMVFIIPIALLVNSIVDGSGPLIKAISS
                     GDMTLPDLAWLNTIPVIGAKLYAGWHNLLDMGGTAIMAKVRPYIGTTTTWFVGQAAHI
                     GRFMVHCALMLLFSALLYWRGEQVAQGIRHFATRLAGVRGDAAVLLAAQAIRAVALGV
                     VVTALVQAVLGGIGLAVSGVPYATLLTVLMILSCLVQLGPLPVLIPAIIWLYWTGDTT
                     WGTVLLVWSGVVGTLDNVIRPMLIRMGADLPLILILSGVIGGLIAFGMIGLFIGPVLL
                     AVSWRLFAAWVEEVPPPTDQPEEILEELGEIEKPNK"
     gene            9994..13050
                     /gene="ydiJ"
                     /locus_tag="ACHOSC_RS09370"
                     /old_locus_tag="pgaptmp_001874"
     CDS             9994..13050
                     /gene="ydiJ"
                     /locus_tag="ACHOSC_RS09370"
                     /old_locus_tag="pgaptmp_001874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding and (Fe-S)-binding domain-containing
                     protein"
                     /protein_id="HGU4547823.1"
                     /translation="MIPQISQAPGVVQLVLNFLQELEQQGFTGDTATSYADRLTMSTD
                     NSIYQLLPDAVVFPRSTADVALIARLAAQERYSSLIFTPRGGGTGTNGQALNQGIIVD
                     MSRHMNRIIEINPEEGWVRVEAGVIKDQLNQYLKPFGYFFAPELSTSNRATLGGMINT
                     DASGQGSLVYGKTSDHVLGVRAVLLGGDILDTQPLPVELAETLGKSNTTIGRIYNTVY
                     QRCRQQRQLIIDNFPKLNRFLTGYDLRHVFNDEMTEFDLTRILTGSEGTLAFITEARL
                     DITPLPKVRRLVNVKYDSFDSALRNAPFMVEARALSVETVDSKVLNLAREDIVWHSVS
                     ELITDVPDKEMLGLNIVEFAGDDEALIDERVNALCVRLDELIASQQAGVIGWQVCREL
                     AGVERIYAMRKKAVGLLGNAKGAAKPIPFAEDTCVPPEHLADYIAEFRALLDSHGLSY
                     GMFGHVDAGVLHVRPALDMCDPQQEILMKQISDDVVALTAKYGGLLWGEHGKGFRAEY
                     SPAFFGEELFAELRKVKAAFDPHNRLNPGKICPPEGLDAPMMKVDAVKRGTFDRQIPI
                     AVRQQWRGAMECNGNGLCFNFDARSPMCPSMKITQNRIHSPKGRATLVREWLRLLADR
                     GVDPLKLEQELPESGVSLRTLIARTRNSWHANKGEYDFSHEVKEAMSGCLACKACSTQ
                     CPIKIDVPEFRSRFLQLYHTRYLRPLRDHLVATVESYAPLMARAPKTFNFFINQPLVR
                     KLSEKHIGMVDLPLLSVPSLQQQMVGHRSANMTLEQLESLNAEQKARTVLVVQDPFTS
                     YYDAQVVADFVRLVEKLGFQPVLLPFSPNGKAQHIKGFLNRFAKTAKKTADFLNRMAK
                     LGMPMVGVDPALVLCYRDEYKLALGEERGEFNVLLANEWLASALESQPVATVSGESWY
                     FFGHCTEVTALPGAPAQWAAIFARFGAKLENVSVGCCGMAGTYGHEAKNHKNSLGIYE
                     LSWHQAMQRLPRNRCLATGYSCRSQVKRVEGTGVRHPVQALLEIIK"
     gene            13047..13457
                     /gene="menI"
                     /locus_tag="ACHOSC_RS09375"
                     /old_locus_tag="pgaptmp_001875"
     CDS             13047..13457
                     /gene="menI"
                     /locus_tag="ACHOSC_RS09375"
                     /old_locus_tag="pgaptmp_001875"
                     /EC_number="3.1.2.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-dihydroxy-2-naphthoyl-CoA hydrolase"
                     /protein_id="HGU4547824.1"
                     /translation="MIWKRKITLEALNAMGEGNMVGFLDIRFEHIGDDTLEATMPVDS
                     RTKQPFGLLHGGASVVLAESIGSVAGYLCTEGEQKVVGLEINANHVRSAREGRVRGVC
                     KPLHLGSRHQVWQIEIFDEKGRLCCSSRLTTAIL"
     gene            13557..13745
                     /gene="ydiH"
                     /locus_tag="ACHOSC_RS09380"
                     /old_locus_tag="pgaptmp_001876"
     CDS             13557..13745
                     /gene="ydiH"
                     /locus_tag="ACHOSC_RS09380"
                     /old_locus_tag="pgaptmp_001876"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416200.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdiH family protein"
                     /protein_id="HGU4547825.1"
                     /translation="MSTQLDPTQLAIEFLRRDQSNLSPAQYLKRLKQLELEFADLLTL
                     SSAELKEEIYFAWRLGVH"
     gene            14293..14661
                     /gene="sufA"
                     /locus_tag="ACHOSC_RS09385"
                     /old_locus_tag="pgaptmp_001877"
     CDS             14293..14661
                     /gene="sufA"
                     /locus_tag="ACHOSC_RS09385"
                     /old_locus_tag="pgaptmp_001877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly scaffold SufA"
                     /protein_id="HGU4547826.1"
                     /translation="MDMHSGTFNPQDFAWQGLTLTPAAAIHIRELVAKQPGMVGVRLG
                     VKQTGCAGFGYVLDSVSEPDKDDLLFEHDGAKLFVPLQAMPFIDGTEVDFVREGLNQI
                     FKFHNPKAQNECGCGESFGV"
     gene            14670..16157
                     /gene="sufB"
                     /locus_tag="ACHOSC_RS09390"
                     /old_locus_tag="pgaptmp_001878"
     CDS             14670..16157
                     /gene="sufB"
                     /locus_tag="ACHOSC_RS09390"
                     /old_locus_tag="pgaptmp_001878"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416198.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufB"
                     /protein_id="HGU4547827.1"
                     /translation="MSRNTEATDDVKTWTGGPLNYKEGFFTQLATDELAKGINEEVVR
                     AISAKRNEPEWMLKFRLNAYRAWLEMEEPHWLKAHYDKLNYQDYSYYSAPSCGNCDDT
                     CASEPGAVQQTGANAFLSKEVEAAFEQLGVPVREGKEVAVDAIFDSVSVATTYREKLA
                     EQGIIFCSFGEAIHDHPELVRKYLGTVVPGNDNFFAALNAAVASDGTFIYVPKGVRCP
                     MELSTYFRINAEKTGQFERTILVADEDSYVSYIEGCSAPVRDSYQLHAAVVEVIIHKN
                     AEVKYSTVQNWFPGDNNTGGILNFVTKRALCEGENSKMSWTQSETGSAITWKYPSCIL
                     RGDNSIGEFYSVALTSGHQQADTGTKMIHIGKNTKSTIISKGISAGHSQNSYRGLVKI
                     MPTATNARNFTQCDSMLIGANCGAHTFPYVECRNNSAQLEHEATTSRIGEDQLFYCLQ
                     RGISEEDAISMIVNGFCKDVFSELPLEFAVEAQKLLAISLEHSVG"
     gene            16167..16913
                     /gene="sufC"
                     /locus_tag="ACHOSC_RS09395"
                     /old_locus_tag="pgaptmp_001879"
     CDS             16167..16913
                     /gene="sufC"
                     /locus_tag="ACHOSC_RS09395"
                     /old_locus_tag="pgaptmp_001879"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008500629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly ATPase SufC"
                     /protein_id="HGU4547828.1"
                     /translation="MLSIKDLHVSVEDKAILRGLSLAVRPGEVHAIMGPNGSGKSTLS
                     ATLAGREDYEVTGGTVEFKGKDLLALSPEDRAGEGIFMAFQYPVEIPGVSNQFFLQTA
                     LNAVRSYRGQETLDRFDFQDLMEEKIALLKMPEDLLTRSVNVGFSGGEKKRNDILQMA
                     VLEPELCILDESDSGLDIDALKVVADGVNSLRDGKRSFIIVTHYQRILDYIKPDYVHV
                     LYRGRIVKSGDFTLVKQLEEQGYGWLTEQQ"
     gene            16888..18159
                     /gene="sufD"
                     /locus_tag="ACHOSC_RS09400"
                     /old_locus_tag="pgaptmp_001880"
     CDS             16888..18159
                     /gene="sufD"
                     /locus_tag="ACHOSC_RS09400"
                     /old_locus_tag="pgaptmp_001880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufD"
                     /protein_id="HGU4547829.1"
                     /translation="MAGLPNSSNALQQWHHLFEAEGTKRSPQAQQHLQQLLRTGLPTR
                     KHENWKYTPLEGLTNSQFVSIAGEISPQQRDALALTLDAVRLVFVDGRYVSALSDATE
                     GSGYEVSINDDRQGVPDAIQAEVFLHLTESLAQSVTHIAVKRGQRPAKPLLLMHITQG
                     VAGEEVNTAHYRHHLDLAEGAEATVIEHFVSLNDARHFTGARFTINVAANAHLQHIKL
                     AFENPVSHHFAHNDLLLADDATAFSHSFLLGGAVLRHNTSTQLNGENSTLRINSLAMP
                     VKNEVCDTRTWLEHNKGFCNSRQLHKTIVSDKGRAVFNGLINVAQHAIKTDGQMTNNN
                     LLMGKLAEVDTKPQLEIYADDVKCSHGATVGRIDDEQMFYLRSRGINQQDAQQMIIYA
                     FAAELTEALRDEGLKQQVLARIGQRLPGGAR"
     gene            18156..19376
                     /gene="sufS"
                     /locus_tag="ACHOSC_RS09405"
                     /old_locus_tag="pgaptmp_001881"
     CDS             18156..19376
                     /gene="sufS"
                     /locus_tag="ACHOSC_RS09405"
                     /old_locus_tag="pgaptmp_001881"
                     /EC_number="2.8.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_function: GO:0031071 - cysteine desulfurase activity
                     [Evidence IEA];
                     GO_process: GO:0006534 - cysteine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase SufS"
                     /protein_id="HGU4547830.1"
                     /translation="MTFSVDKVRADFPVLSREVNGLPLAYLDSAASAQKPSQVIDAEA
                     EFYRHGYAAVHRGIHTLSAQATEKMENVRKRASLFINARSAEELVFVRGTTEGINLVA
                     NSWGNSNVRAGDNIIISQMEHHANIVPWQMLCARVGAELRVIPLNPDGTLQLETLPTL
                     FDEKTRLLAITHVSNVLGTENPLAEMITLAHQHGAKVLVDGAQAVMHHPVDVQALDCD
                     FYVFSGHKLYGPTGIGILYVKEALLQEMPPWEGGGSMIATVSLSEGTTWTKAPWRFEA
                     GTPNTGGIIGLGAALEYVSALGLNNIAEYEQNLMHYALSQLESVPDLTLYGPQNRLGV
                     IAFNLGKHHAYDVGSFLDNYGIAVRTGHHCAMPLMAYYNVPAMCRASLAMYNTHEEVD
                     RLVTGLQRIHRLLG"
     gene            19389..19805
                     /gene="sufE"
                     /locus_tag="ACHOSC_RS09410"
                     /old_locus_tag="pgaptmp_001882"
     CDS             19389..19805
                     /gene="sufE"
                     /locus_tag="ACHOSC_RS09410"
                     /old_locus_tag="pgaptmp_001882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfuration protein SufE"
                     /protein_id="HGU4547831.1"
                     /translation="MALLPDKEKLLRNFLRCANWEEKYLYIIELGQRLPELRDEDRSP
                     QNSIQGCQSQVWIVMRQNAQGIIELQGDSDAAIVKGLIAVVFILYDQMTPQDIVNFDV
                     RPWFEKMALTQHLTPSRSQGLEAMIRAIRAKAAALS"
     gene            19954..20958
                     /gene="ldtE"
                     /locus_tag="ACHOSC_RS09415"
                     /old_locus_tag="pgaptmp_001883"
     CDS             19954..20958
                     /gene="ldtE"
                     /locus_tag="ACHOSC_RS09415"
                     /old_locus_tag="pgaptmp_001883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase LdtE"
                     /protein_id="HGU4547832.1"
                     /translation="MKRASLLTLTLIGAFSAIQAAWAVDYPLPPTGSRLVGQNQTYTV
                     QEGDKNLQAIARRFDTAAMLILEANNTIAPVPKPGTTITIPSQLLLPDAPRQGIIVNL
                     AELRLYYYPPGENIVQVYPIGIGLQGLETPVMETRVGQKIPNPTWTPTAGIRQRSLER
                     GIKLPPVVPAGPNNPLGRYALRLAHGNGEYLIHGTSAPDSVGLRVSSGCIRMNAPDIK
                     ALFSSVRTGTPVKVINEPVKYSVEPNGMRYVEVHRPLSAEEQQNVQTMPYTLPAGFTQ
                     FKDNKAVDQKLVDKALYRRAGYPVSVSSGATPAASNAPSVESAQNGEPEQGNMLRATQ
                     "
     gene            complement(21022..>21255)
                     /gene="lpp"
                     /locus_tag="ACHOSC_RS09420"
                     /old_locus_tag="pgaptmp_001884"
     CDS             complement(21022..>21255)
                     /gene="lpp"
                     /locus_tag="ACHOSC_RS09420"
                     /old_locus_tag="pgaptmp_001884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein lipoprotein Lpp"
                     /protein_id="HGU4547833.1"
                     /translation="KATKLVLGAVILGSTLLAGCSSNAKIDQLSSDVQTLNAKVDQLS
                     NDVNAMRSDVQAAKDDAARANQRLDNMATKYRK"
BASE COUNT         5510 a         5195 c         5392 g         5158 t
ORIGIN      
        1 cagcgtcagt tatatttgcc atgaatttac caaagagtat cgtatttccc ctattaacta
       61 tgttattcaa cggcgtatga cggaagcaaa atggtcactc actaatactg aattatcaca
      121 ggcagagatc tcctggcgtg tgggttatga aaatgtcgat cactttgcca aactgttttt
      181 gcgccatgtc ggttgttcgc ccagcgatta ccgtagacaa tttaaaaact gttttgcgga
      241 acaagaaatc ctatctgaat ttcctcaacc ggtaagtctt gttggataaa tctggcggcg
      301 cagtttgtta agaagctgcg ccgcctgcag attatttgtt ctgatagtct ttcaggatct
      361 gccgacctgc tacgtaaatc ataatttcgt ctgtaccgcc gccgatacgt tcacaacgga
      421 catcacgcca gaagcgggag acgcgcgctt catcggtata gcccagaccg cccatgattt
      481 gaatcgcatc atcaatgact tccattgcgg tacgtgcgca atacagtttt gccagcgccg
      541 cgctggtgcg cagtgactga tgctgatcgg cttgccatgc cactttcagc accatgttgc
      601 gcatgttgtc gatcttaatc gccatcagcg ccagtttttc ctggatcatc tggttatgac
      661 caatgggctt accaaaagcg atacgttggt tggcatagcg ggcggcatct tcaaaggcgc
      721 attcggcaaa gccggtgctg cgcgcggcgt tgatcaggcg ctccatctca aagttgtaca
      781 tcacattgag gaaacccatt ccttcttcgc ccaccatgtc gctctcttca acttccacat
      841 tgtcgagata gacttcgcag gtgctgagca tatgccagcc gattttatgc agcgggttaa
      901 tcttaatgcc gggcttactg gagtcgaccc accacagggt gaaggctttt ttgggatctt
      961 tcggttgcgg atcgcgcgcc agcaccagca tatacgggta ctctttcgca ctggtaataa
     1021 aggttttttg tccgttgatg tagaccttgc cgtttttacg cgtgtaagtg gtagtggcac
     1081 tgttgttatc tgagccagca cctggttccg tcaacgccag ggcataggcg ggatcacccg
     1141 tttccagggt gctctctgcc gttttacgta gctgctctgc agaaccgaaa cggcgcatac
     1201 tgtgaataca ttgaccgttg gtaattaaaa atgccggagc accgcatttt gacacttcca
     1261 tcagcgccag catttgggtg acgtaatccg cagggatacc accaaattct tccggcacgc
     1321 caagcatgga aataccgtta tccgccagcg cccgcataaa ctcacgcgga tatgtcccgt
     1381 tttgatcgca ggtgcggaaa tactcttccg gaaagttagt cgtaatcagt tcgcgaatac
     1441 tggccagcag cagttcttgt tcttcagtta aagaaaaatc catcattata ctcctgttaa
     1501 tgagccgctt caggcaggac aaaacccatc gccgcatcga taaataatct ttcgtccatc
     1561 agtttgagtt ctggcgaaat cactggggtg aagtccattt tgtcgagaat atctttttgt
     1621 aaatcgacgc caggggcgat ttcgattaaa tgcaggccgt cttctttcag cgtgaataca
     1681 gcacgttcgg tgatgtaacg aacatccagt ccccgttcga gcgcgatttt tccgctaaaa
     1741 gtaatttccg gtagttcccg aataaattta tttacccgtc cttcctggac gatatgcaat
     1801 ttgccatcgg caatttctgt ttttaaactg cccgcagtta acgtgccgca gaaaataatt
     1861 ttcttcgagg tggcactgat atcaataaat ccaccggtac ccatgatttt gccattgaat
     1921 ttatgcacgc cgacgttacc gtgctggtcg acttcagcaa aactcaaata acaaacatcc
     1981 agaccaccac cgtgataaaa atcaaactgg gacgtcatat ccagaatggc gcgggtattc
     2041 acgttcgcgc caaaggcgat accctgtgaa gtaattccgc caatcggacc tgtttctacc
     2101 gtcagaataa agtcatcagc acaaccttct tcccgggcga ccaggccaat accgtcagca
     2161 ataccgacgc cgacattccc caccgcgcct ttacgcattt cgaataatgc acgccgcgca
     2221 actaatttac gttggtttag gggcaggcta agtttgtgct gtcatcaagg gtgaagtcac
     2281 cagaaataaa acggttaacc ggcgcaccgc catacagttg gctttgatcc ggatcgacca
     2341 ccacaatatc caccagataa cccggaatac ggacagattt aggatgcagc gtggctttct
     2401 taaccatttt ctgcacctgc atcatcacaa taccgccgtt attgtgcacc gcctgggcaa
     2461 taaccaatgc gtcgagatac atcacctcat cttcaaaagt ggcgtagcct tcactgtcgc
     2521 aggtggtagc gcgaatgaag gcgatatctg gcgcaatcgc tttgtaatag agatattctt
     2581 tgttatcaaa ctcgaccagt ttaatcaggt cttctttcgt gacttcattc agtttgccgc
     2641 cttgctggcg tggatcgaca aatgtcccga tgccaatatc actaataata ccaggctggt
     2701 gggctgcggc ggcgcgtaag gtttgtgtaa gtacaccttg tggatagtta taagcaataa
     2761 ttttattttg ttctgcgagt tcagaaatac gcggcgattg tccccagtga ccacataatg
     2821 cccatttcac cagaccttct tgcgccagag gactaatacc acggtcggcg cgatcgccaa
     2881 gccctgtcgg actaataatc gataaattac gtggcgtttg agtctgttta tatttatcag
     2941 caagagctgt aattaacgtg gtggcttcca gaataccgcc gccagcgcct aacacacaaa
     3001 gtgttgcttc gtcgggaata taattcaccg cttcctgtgc cgacaggacc ggcactcgtc
     3061 cattaatacg aggtggtttt acaggtttca ttgttaattc ctgattcaga tgtgattttc
     3121 cctacgccag atggagaatt agcgcacaga gactcacgaa atatttttat taaatacggt
     3181 taaatatatt ttttaagttc ggcggggagg gtgttcccgc cgaaatatta ttgcttatgc
     3241 ctggtgtaaa atagttaata ccgtgcgtaa atcatttacc gagatttgcc ctggtgcaga
     3301 ggcttttttt accgcaccaa aagttgccgc cgagccaaat acttcaccag ccagacgaga
     3361 aattacgcca gtttttgcca tcgacatcgt gatgattgga cgatcggcat actgctcctg
     3421 catctccagg gtcgcggtaa gcaacgtcag cacatcgctg gtactttgcg gcatcagcgc
     3481 aatcttagga atatcggcgt cgaaggactg cattttgcgc agacgggcaa tgatttcttc
     3541 ggcttccggc gttttatgga agtcatggtt ggacatgaca actttcacat catgcgcgtg
     3601 ggcgtaggcg acggtttctt taacctgatc atcgccggta aataactcca gatcgatcat
     3661 atcaaccagg ccgctgtcga tggctgcacg attgagagca atataagcct cggtggaaat
     3721 cgcctgctcg ccgccttctt tggcactgcg gaaggtaaac agcagcggtt tttctggcat
     3781 ggtttcacgg agaatttttg ccgccgccat gacagactcc acattggaga ggtcggcaaa
     3841 gtggtccaca cgccattcca gaatatcaaa gtccgcttca cgataggcga gagcttcgga
     3901 tttcacgcgg gcgatatctt tcgccatcag cgagacgatg attttaggtg cgcccgtacc
     3961 aatgacgaga tcttttacag ttacggtttt cattttttac cctttctgca cgcggtcagc
     4021 ctgtcaggca ccgaacccca tgacctgttt aacatattcc agagggaaat ctttgccagt
     4081 ccataacgtg aactgttcag ccccttgcca caacaacatg ccgtatccat caatcgtttt
     4141 gcaaccagct tgttgcgcct gctgcaataa cttcgtcata tgcgggttat acacgcattc
     4201 agtgaccagc agtcccggat gtaacagact gatatcatta actaatgatt cattctcaag
     4261 gggtttcata cccacttttg tgccattggt taaaatgtcg gcggaagcca gggcttcagc
     4321 aaaggcttgc tgatcggcga gatcggtaac cgtgacgaca caatcggtgt tttcgttaac
     4381 ccgctgcgcg aaggcgaggg ctttatcgaa gaactcatcc cgacggttaa agagtttaat
     4441 ttcttttaaa ccttcaattg ccccctgcgc gccaatcgcc gttgaggcac ctccggcccc
     4501 taacagcacc attgttttgc ctttgatatc aaaaccgctc tctttaatgg cgcgaatatg
     4561 gcccgtgccg tcggtgttat agccgcgcag gtagccatca tcattaacga tggtgttgat
     4621 ggcacccacc agtttggctg ctggcgttaa ttcatcaaca tattcacacg ccagttgttt
     4681 gttcggcatt gatataccag ttccgcgcat tttgagggct tttaatcctt caattgctcc
     4741 gggaaaacta tcgttatcca cttcaaaggc catataggta aatggcaatc ccgctttttc
     4801 taaggcttta ttctgcattt caggcgataa actgtggcgg ataggatagg ccatcagccc
     4861 aatcaattcg tattttgcgg taacatccat aattaactcc tttaacctct atgcttaatt
     4921 gtgtttaacc gggtgctaaa tttacgctcg ccaaaccgca catcattctc aggaataata
     4981 aaaacgcggt aatagcggat aaaaacaata attgcggtaa tcagggccag cagcgcgaaa
     5041 gtaaaatcga gaacaatgat atattgaagg ccgatgttcg acagataacc ggtaatcagc
     5101 ggaataacaa agttagccag tccacccatc atcatataaa tactggtgac tttggctttg
     5161 ctttttggga aaaactctga catgaccgaa acaccgagct gtaaaatgcc gccagctgct
     5221 gagaaaccga taacaaaggc gccggcatta cacaccagtg gagaagggta caggtagata
     5281 atggctgctg ttattgttgc cagtgcagag ttaaatacat tagcccagat gggccggacc
     5341 atttttttca gtagtgcggc aaaaataaag acacagacca acgagcccat actgtaataa
     5401 gagatggttt ttaatgcctc agcttctgac ataccagcaa aagccatcgc atatttgggc
     5461 atccacacca caatcacata aaaggtcgag aatgcggcta caccgaacag taccgatgaa
     5521 acaccttcca gccagactaa cggtttgctg ttcatttgcg gtaattcatt agctacgctg
     5581 gcgtccacca actggctggg gaatttgctt ttcaacagca tcagcgtgat cagtacaaac
     5641 agaatacccg gaataatcag cccatagccg taccagatat tattgagcaa catatagctc
     5701 accagcattg ggtagaacat ttgcccaaat gacaccatcg ctttaaccag tatgaccgcc
     5761 gaaccagagg ctttcggaaa gcattccatg agcgcggggt agccacccgt atccagcgct
     5821 gagttagcga tacctacgca cactgccaga ccgtaggcga gagttaaatt cgggcaagcg
     5881 ggaataccaa agaagaatag cagatacatt attactgcca ttaatatcac cgcccgacga
     5941 ccaaacttat cggagatcac accgaagaat aaaatactga tcaatcgccc caaaccaata
     6001 ccggaaatta agtaggcaat gcccgcgttg tcagtggaaa acttttccgc cagagatgac
     6061 atgttttggg caagcgtaat aacactaatg ccgtgcagga agtagctgaa gtaaatacaa
     6121 agaacagcca ggataaatgg cgtgctgaaa gccttatttt gagacatttt cacagctcct
     6181 tgctgaaaga taatttcgtt accgtacgta ttgttcttcg acggtttaca taattactat
     6241 actgaagatg attttccctg ggtacatggc ataaagttta tgtttccagg aatattgcat
     6301 gtcatagttt gtttttgatc gcgattttcc ttagttagcg cagaaagagt tatttatccg
     6361 taagatcttg catgacctga aaagaatatt gcattaccca cccggagcga cattttcctt
     6421 tagcgagtga tgccgcgttt ttttgcggct gcgtaagccg ataaacagtg ccagtaaaaa
     6481 accgatggca gcgatggcgg tatcgaacca cataatatcg gcaatacttc tttgggacag
     6541 atgagccgta atcaacggaa tagtaaaggt cgcaatactg cccgcactgt aatagatccc
     6601 tgtagcttta cctttagcgt aagggaaacg ttcagccatt aacgtcaggc caatttgcac
     6661 aacacctcca gcagaggtaa aaccaatgac aaaagcaaat attatcacca cataaaatgt
     6721 gggatgcagg cagacggtaa acagagcaat aaatgagata aaggtgtaca gcatcagtaa
     6781 tgttgttggg cgaacggtat tacgaatgag tggagcggta ataaatacac aaagcagcga
     6841 atccacggtg tagatactga gtagtttaat cgacatagtg tatgacatgc ctgcaacaaa
     6901 ttgtccgtac tgtgccagcc actggctaac cagataaaac gttgccattg agatatagcc
     6961 atataaggta taactggcta aatcgataat tgaacagcga tgttccgtag agctggtggt
     7021 tttctttatg acaggtaagc gatgacccgg atggggtggg aacgtacaac ggtataaaaa
     7081 cagagcgtta ataaacataa tgcctgcagc aatcataaag gaccaaccga accacagttc
     7141 agcccacact aacaggctaa tgattagcgg taataaaaat tgtccgctgg aaacaaatgc
     7201 tttaattaaa atattggctg tcccaggtga gcgtggaaaa gcttccatca aactgggata
     7261 agtgcctgca tcgagaaaac tgtttgccat tcctgccaga aagccaaaaa cataagcgat
     7321 aatgatgtta ttggtgtgca ggatgccaaa aaagaaggcc atatagcagc acatcccgag
     7381 catgataaaa gggcggcgac caaagcgatc ggataataat cctgcaaaaa gcaagacact
     7441 taatcgacca atgcccagcg atgagataac tatcgagaca cccgcggcat tagtctgcca
     7501 aagtgtctcc agcgaggcca tattcaggct catcaaaatg acgcccatac catgcactag
     7561 gtaattaaaa tacaacccaa gtgcggtagg gtaataggga tttttcatag gttctactta
     7621 gcaaaggtgc tttaaaaagt gtagcgatgt tgtgtcagta tccgttcatt attgttgtaa
     7681 caacgacaat tcctttttgt ggagaaatta acaaagccgc tccagatcgt gtgcatataa
     7741 ttcagcggct accgattgat agatcttcca gtcaataaaa ttgccatcag ggtagacttg
     7801 ttgaaacgcg gttaagtcgg ggaaaaaacg catggtgttg ttgccgatgc ggttatttat
     7861 tttctctatg atagcatgaa gatgtttttt tcgttgctga cgcatagcca acagttgctc
     7921 gactacacga tcaggaatgg cgcatttgcc attttcccac tgacgccagc tttcactgtc
     7981 accggtctgg gcaatccagg tcgcgcattc atcaatagtc atggcaaaaa tatggcgaag
     8041 tgcctggagt tcataagcgt tcatcgtgta atggctgcat gaaataaaca ataaaatata
     8101 aataacatta tcagcctgct gacggcttga agagagtcac agtatcttgt gcaacattat
     8161 tggaaagagt gaggggcgag gtagcgaagc ggaaaaatgt taaaaaaaag cccatcgtgg
     8221 gagatgggca aagactacac acagcaattc gttgtttcac tcaggggatt tccatgctta
     8281 taaatcaata tgttgattta taaccgtaag ctaatagtag gcatacggac tttttgcgtc
     8341 gatcagattc gtctcattag tttaaaaact gtaaagaatt tgcgacaggt actaccagct
     8401 gaatggcggt aagcaggaaa atatggggaa gagcaagcaa atatcaatta gcgggggtta
     8461 acaatcaggc ggcagagccg cctgatgaga aattacttat tcggtttctc gatttcgcca
     8521 agttcttcga gaatttcttc cggttggtcc gtcggcggcg ggacttcttc cacccacgcg
     8581 gcaaacagac gccaggaaac ggctaacaga accggaccaa taaacagacc gatcatcccg
     8641 aaagcaatca aaccaccaat aacaccagag aggatcagga tcagcggtaa atcggcaccc
     8701 atgcgaatta acattgggcg gatgacgtta tccagcgtgc caaccacacc gctccacact
     8761 aacaataccg ttccccaggt ggtatcgcca gtccagtaga gccagataat cgccggaatc
     8821 agtaccggca acgggccaag ctggacaagg caggagagga tcattaacac cgttagcaac
     8881 gttgcataag gtacgccgga tacggcgagg ccgataccgc caagcactgc ctgtactaac
     8941 gccgtcacca ccacacccag cgccaccgcg cggatagcct gtgccgccag cagtacggcg
     9001 gcatctccgc gaacgcctgc cagacgggtt gcaaaatggc gaatgccttg tgccacctgt
     9061 tcaccgcgcc agtacagcag ggcactgaag agaagcatca gcgcacaatg caccataaag
     9121 cgcccgatat gcgccgcctg cccaacgaac caggtggtgg tggtgccaat ataagggcgg
     9181 actttcgcca tgatcgccgt cccccccata tccagcaagt tgtgccagcc tgcatacagc
     9241 ttcgcgccaa tcaccggaat ggtattaagc cacgccagat cgggtaacgt catgtcgccg
     9301 ctggaaatgg ctttaattag cgggccgctg ccgtcgacga tactgttaac cagcaaagca
     9361 atagggatga taaacaccat cactaataac agcgtcatca ccagaacggc gagagagcgg
     9421 cggccaaaca tgatcttttg caaacgtaac aataccggcc aggtggcgat aaccaccgta
     9481 ccggcccatg caaagccgag aataaagggt tgaacaatcc acagacatgc cacaatcatg
     9541 atggctaaaa acagcaccga aagcagaatt tgtgcgacat ccctgggctg acgaacattt
     9601 accataacta cttttcacct tttttgtgcg tcaaatcgtc gacgcgacgt gaacacgcga
     9661 aactcacctg cataatgatg agcaatttca gcggttttta acaggccgat tctgccctta
     9721 attctgatgg gcgtgttaga aaaaaatgtg gtacaacaac tcaggcaaca cgcaaacgat
     9781 ttactcgtcg tatttcaagt tgcatgtgct gcggctacgt tcgttcaccc cagtcactta
     9841 ctattgtaag ctcctggagt tcactcgctt gccgccttcc tgcaactcga aatccataga
     9901 gtaaataaac atcacctttc agtgcgctat tgcagccgcc tgaaagaata gtgtatcgac
     9961 ctacatcaca gaccgcagga aagggtcaat ataatgattc cacagatttc ccaggcaccc
    10021 ggcgtcgttc aactggtgct taattttttg caagagctgg agcaacaagg ttttaccggc
    10081 gatacggcga caagttatgc cgatcgtctg acaatgtcga ccgacaacag tatttaccaa
    10141 cttctccccg atgcggtggt atttccgcgt tcaaccgcag atgtggcgct gatcgcccgt
    10201 cttgccgcgc aggaacgcta ttcatcgctg atctttaccc cccgtggcgg cggcaccggc
    10261 actaacggtc aggcgctcaa ccaggggatt attgttgata tgtcccgcca tatgaaccgc
    10321 atcatcgaaa ttaaccctga agagggctgg gtgcgcgttg aggccggggt gataaaagat
    10381 caactcaatc agtacctgaa accgttcggc tacttttttg cgccggaact ttcgaccagc
    10441 aaccgggcaa cgctcggtgg gatgatcaat accgatgctt ctggtcaggg atcgctggtc
    10501 tatggcaaaa cgtcggatca cgtacttggc gtacgtgcgg tgttgttggg gggcgatatt
    10561 ctcgatacgc aacctttacc cgtcgaactg gcggaaacgc tgggtaaatc caataccaca
    10621 atcgggcgaa tttataacac ggtttatcaa cgttgccgtc agcaacgcca gttaatcatc
    10681 gacaacttcc ccaaacttaa ccgctttctt accggttacg atctgcgtca tgtctttaac
    10741 gatgagatga ccgagttcga cctgacgcgc attctgacgg gttcagaagg gacgctggcc
    10801 tttattaccg aagcgcggct ggatattacg cccttgccta aagtgcgccg tctggtgaac
    10861 gtcaaatatg actcttttga ctccgcgctg cgtaacgcgc cgtttatggt tgaggcgcgg
    10921 gcgctttcgg tagagacggt ggactcaaaa gtgctgaatc tggcgcggga agatattgtc
    10981 tggcattccg tcagcgagtt gattaccgat gtgcctgaca aagagatgct cgggctgaac
    11041 attgtggagt ttgctggtga tgatgaggcg ctgattgacg agcgagttaa tgctctctgt
    11101 gtgcggcttg atgagctgat tgccagccaa caggcaggtg tgattggctg gcaggtgtgc
    11161 cgcgagctgg cgggtgttga acgtatctat gcgatgcgta aaaaagccgt tggtctgctt
    11221 ggcaatgcca aaggtgccgc taagccaatt ccgtttgctg aggatacctg cgtaccgccg
    11281 gaacatctgg ccgattatat tgctgaattt cgcgcgctgc tcgacagcca cggcttaagc
    11341 tacggtatgt tcggtcacgt cgacgcaggt gtcttgcacg tccgtccagc gctggatatg
    11401 tgcgatcctc aacaagagat tttgatgaag caaatctctg atgacgtagt ggcgctgact
    11461 gcgaaatacg gtggtttgtt gtggggcgag cacggcaaag gttttcgcgc tgaatacagc
    11521 ccggcgtttt tcggtgagga actttttgca gaactgcgca aagtgaaagc ggcatttgac
    11581 ccgcataacc gactcaaccc agggaagatt tgcccgccag aaggtctcga tgcgccgatg
    11641 atgaaagtgg acgcggtgaa gcgcggtaca ttcgatcggc agatccccat tgcggtacgc
    11701 cagcagtggc gcggtgcgat ggagtgtaac ggcaacggtt tatgcttcaa ctttgatgcc
    11761 cgtagtccga tgtgtccgtc gatgaagatc acccagaacc ggattcattc accgaaaggg
    11821 cgcgcaacgc tggtgcgtga atggctgcgt ttgttggcgg atcgcggcgt tgatccactc
    11881 aaactggaac aagaactgcc tgaatccggc gtcagtttgc ggacgttaat tgcccgcacg
    11941 cgcaatagct ggcatgcgaa taaaggcgaa tatgacttct cacacgaagt caaagaggcg
    12001 atgtcgggct gtctggcctg taaagcgtgt tcgacccagt gccccatcaa aattgatgtg
    12061 ccggagtttc gctctcgttt tctgcagctc tatcacaccc gttatttacg cccgctgcgc
    12121 gaccacctcg tcgctacggt cgagagctac gcgccgctga tggcacgcgc gccgaaaacc
    12181 tttaacttct tcattaacca gccgctggtg cgcaaactct cggaaaaaca tatcggcatg
    12241 gttgatttgc cgctgctgtc ggtcccctcg ctacaacaac aaatggtggg gcatcgctcg
    12301 gcaaacatga cgctggaaca gcttgaatct ctcaatgcag agcagaaagc gcgcacagtg
    12361 ttggtggtgc aggacccctt taccagctat tacgatgcgc aagtggtggc ggattttgtc
    12421 cgtctggtcg aaaaattagg tttccagcct gtgttactgc cattttcgcc aaatggcaaa
    12481 gcccagcata ttaaaggttt tcttaatcgt tttgcgaaga cggcgaaaaa gacggcggat
    12541 ttcctcaacc gtatggcgaa gctgggtatg ccaatggtgg gcgtcgatcc ggcgctggta
    12601 ctttgttatc gcgatgaata taaactggcg ctgggcgagg aacgtggcga gtttaacgtc
    12661 ttactggcga atgaatggct ggcaagcgca cttgagtcac agccagtggc tacagtcagc
    12721 ggtgaatcat ggtatttctt tggtcactgt accgaagtta ccgccttgcc gggtgcgcca
    12781 gcacaatggg ccgcgatatt tgcccgtttt ggcgcgaaac tggaaaatgt cagcgtgggt
    12841 tgctgcggca tggcagggac ttacggacat gaagcgaaaa accataaaaa ttcgctcggg
    12901 atctatgagt tatcctggca tcaggctatg cagcgactgc cgcgtaaccg ctgtctggcg
    12961 accggatatt cctgccgtag ccaggtaaaa cgggttgaag gcacgggggt acgccatcct
    13021 gtgcaggctt tactggagat tattaaatga tatggaaacg gaaaatcacc ctggaagcac
    13081 tgaatgctat gggtgaagga aacatggtgg ggttcctgga tattcgcttt gaacatattg
    13141 gtgatgacac ccttgaagcg acaatgccag tagactcgcg gacaaagcag cctttcgggt
    13201 tgctgcatgg aggagcatcc gtggtactgg ccgaaagtat cggttccgtt gccggttatt
    13261 tatgtaccga aggtgagcaa aaagtggttg gtctggaaat caatgctaac cacgtccgct
    13321 cggcacgaga agggcgggtg cgcggggtat gcaaaccgtt gcatctcggt tcgcgtcacc
    13381 aggtctggca gattgaaatc ttcgatgaga aagggcgttt gtgctgttcg tcacgattga
    13441 cgaccgccat tttgtgacgt tgtcaccaga aaagtgtgac gcatattgcc tttggtcatt
    13501 atctgcacag taaacggtgg tatactgtgc aggtcttgtc taaaagcgag gatgctatgt
    13561 ctactcaact tgatcccacc cagctggcca ttgaattttt acgccgtgac cagagcaatc
    13621 tttcacctgc ccaatatctc aaacgtctga aacaactaga acttgaattt gctgacctgc
    13681 ttactctctc ttctgctgaa ttaaaagaag aaatctactt tgcctggcgt ctgggcgtgc
    13741 attgatcctg acttaatgcc gcaagttcgc caaatcgacc tggattaacc tccgtccagg
    13801 ttcgatgacc ttcttaatac tgcttatcta ccgcctgata ttgttcaaaa ataatactaa
    13861 tcgcagtttg tgttaaaacg gcgggttagc tttatgagat tattcttatc gccccttcaa
    13921 gagctaagcc actgagagtg ccggagataa gcgccggatg gggtagaaac ccttaagcct
    13981 gtgttgcaca gacttaaggg ttttcttatt tctgaacgct gcactttttt acctatctct
    14041 taaatagtca ttttcaatac aactttttct ttttcatcac agccttaatg ctccttagat
    14101 aataaccatt atctaacaat gagataccta attcttagcg tgcctgttaa cccacacatc
    14161 agggtctatg cttattaaaa gcaggcaaaa agcatgtttt acggtaaagc ccctgcgttt
    14221 gctgggttgt actgataatc attatcacta acatgctgtt atacgctgaa agcgatgaag
    14281 tgaggtaaat cgatggacat gcattcagga acctttaacc cacaagattt cgcctggcaa
    14341 ggcttaacgc tgacacccgc agcggcgata cacatccgtg agctggtggc aaagcagccg
    14401 ggtatggtcg gcgtgcgctt aggcgtgaag caaacgggct gcgcgggctt tggctatgtg
    14461 ctcgacagtg ttagcgagcc ggacaaagac gatctgctgt ttgaacacga cggcgcgaag
    14521 ctgtttgtcc cgctgcaagc gatgccgttt attgatggca cggaagtcga tttcgttcgt
    14581 gaaggactta atcagatatt caaatttcac aaccctaaag cccagaatga atgtggctgt
    14641 ggcgaaagct ttggggtata ggcggtacta tgtctcgtaa tactgaagca actgacgatg
    14701 tcaaaacctg gaccggcggc ccgctgaatt ataaagaagg attcttcacc cagttagcca
    14761 ccgatgagct ggcaaagggg ataaacgaag aggtggtgcg cgcaatttcg gcgaagcgta
    14821 atgagccgga gtggatgctg aagtttcgtc taaacgccta tcgcgcatgg ctggagatgg
    14881 aagaaccgca ctggttgaaa gcgcactacg acaagctgaa ttatcaggat tacagctact
    14941 actcagcacc atcgtgcggt aattgtgacg acacttgcgc gtctgaacct ggcgcggtgc
    15001 agcaaactgg cgcgaacgcc tttttaagta aagaggtgga ggcggcgttt gagcagttgg
    15061 gcgttcccgt gcgggaaggc aaagaggtgg cggtggatgc cattttcgac tcagtttcgg
    15121 ttgccactac ttatcgcgaa aaactggcgg agcagggaat tattttctgt tcctttggtg
    15181 aggcgatcca cgatcacccg gaactggtgc gtaaatatct cggcaccgtg gtgccgggga
    15241 atgacaactt ctttgccgcg cttaatgcgg cggtagcctc tgacggtacg tttatttatg
    15301 tgcctaaagg cgtgcgctgc ccgatggaac tttccaccta ttttcgcatt aacgcggaaa
    15361 aaaccgggca gtttgagcgc accattctgg tggccgacga agacagctac gtcagctaca
    15421 ttgaaggctg ttccgctccg gtgcgtgaca gctatcagtt acacgcggca gtggtggaag
    15481 tcatcatcca taaaaacgcc gaggtgaaat attccacggt acaaaactgg ttccctggcg
    15541 ataacaacac cggcggtatt ctcaacttcg tcaccaagcg tgctttgtgc gaaggcgaaa
    15601 acagcaaaat gtcatggacg caatcagaaa ccgggtccgc gattacgtgg aaatatccca
    15661 gctgcatttt gcgcggcgat aactccattg gtgagtttta ctcagtggcg ctgaccagcg
    15721 gtcatcagca agcggatacc ggcaccaaga tgatccacat cggtaaaaac accaaatcga
    15781 ccattatctc gaaagggatc tctgccggac atagtcagaa cagttatcgc ggcttagtga
    15841 aaatcatgcc gacggcaacc aatgcgcgca atttcactca gtgcgactca atgctgattg
    15901 gcgctaattg tggggcgcat accttcccgt atgtcgaatg tcgcaataac agcgcacaac
    15961 tggagcacga ggcaacgaca tcacgtattg gtgaagatca actgttttac tgcctgcaac
    16021 gcgggatcag cgaagaagac gccatctcga tgattgttaa cggtttctgc aaagacgtgt
    16081 tctcagaact gccgctggaa tttgccgttg aagcacaaaa actcctcgcc atcagtcttg
    16141 aacacagcgt cggataagga ataaacatgt taagtattaa agatttacac gtcagcgtgg
    16201 aagataaagc tatcctgcgt ggattaagcc tcgccgttcg tcccggcgaa gttcacgcca
    16261 ttatggggcc aaacggttcg ggcaaaagta ccttatcggc aacgcttgcc gggcgcgaag
    16321 attatgaagt gacgggcggc acggttgagt tcaaaggcaa agatttgctt gcactgtcgc
    16381 cggaagatcg tgcaggcgaa ggcatcttta tggccttcca gtatccggtg gagattccag
    16441 gcgtcagtaa ccagtttttc ctgcaaacgg cacttaatgc agtgcgcagc tatcgcggcc
    16501 aggaaacgct cgaccgcttt gattttcagg atttgatgga agagaaaatc gctctcctga
    16561 agatgccgga agatttatta acccgttcgg taaacgttgg tttttccggc ggcgagaaaa
    16621 agcgcaacga tattttgcaa atggcggtgc tggaaccgga gttatgcatt cttgatgagt
    16681 cggactccgg gctggatatt gacgcattaa aagtggtcgc cgatggcgtg aactcgctgc
    16741 gtgatggcaa gcgctcattc atcattgtta cgcactacca acgcattctc gactacatca
    16801 agcctgatta cgtccatgtg ctatatcggg gacgaattgt gaaatccggc gatttcacgt
    16861 tggtcaaaca actggaggag cagggttatg gctggcttac cgaacagcag taacgcgctg
    16921 caacagtggc atcacttgtt tgaagctgaa gggacaaaac gctcaccgca agcacagcag
    16981 catttacaac aattgctgcg taccggactg ccgacacgta aacatgaaaa ctggaaatat
    17041 acgccgctgg aagggctgac caatagccag tttgtcagca ttgcgggaga gatatcccca
    17101 cagcagcgag atgccttagc gttaacgtta gacgctgtgc ggctggtatt tgtcgatgga
    17161 cgttacgtgt cagcactgag cgatgcgact gaaggcagcg ggtatgaagt gagcattaac
    17221 gacgaccgtc agggggtacc cgacgctatt caggcggaag tgtttctgca tttgacggaa
    17281 agcctggcac aaagcgtgac gcatatcgcc gtgaagcgcg gtcaacggcc ggcaaagcca
    17341 ttgctgttaa tgcatattac ccagggcgtg gcaggtgaag aggtcaacac tgcccattac
    17401 cgacatcatc tggatctggc ggaaggtgcc gaagcaacgg tgatcgaaca ttttgtcagc
    17461 cttaatgatg ctcgtcactt caccggcgca cggttcacta tcaatgtcgc agcgaacgcc
    17521 cacttgcagc atatcaagct ggcgtttgaa aacccggtca gtcaccactt tgcccataac
    17581 gatttgttgc tggctgacga tgccaccgca tttagccata gtttcctgct gggtggcgca
    17641 gtgttacgac acaacaccag tacgcaactc aatggcgaaa acagcacgct gcggatcaat
    17701 agcctggcga tgccggtgaa aaacgaggtg tgtgataccc gtacctggct ggaacacaat
    17761 aaaggttttt gtaacagccg acagttgcac aaaactatcg tcagcgacaa aggccgcgcg
    17821 gtatttaacg gtttgatcaa cgtcgcgcag cacgccatca aaacggatgg tcagatgacc
    17881 aacaataatc tgctgatggg caaactggca gaagtggata cgaaaccgca gctggaaatc
    17941 tatgcagatg atgtgaaatg cagccacggc gcgacggtgg ggcgtattga tgatgaacag
    18001 atgttctatc tgcgctcgcg cgggatcaat cagcaggatg cccagcagat gatcatttac
    18061 gcctttgctg ccgaactgac ggaagcactg cgtgatgagg ggcttaaaca gcaggtgctg
    18121 gcccgaatcg gtcaacggct gccaggaggt gcaagatgac tttttccgtc gacaaagtgc
    18181 gggccgactt tccggtgctt tctcgtgagg taaacggttt gccgctggct tatctcgaca
    18241 gcgccgccag tgcgcagaaa ccgagccagg tgattgacgc cgaggccgag ttttatcgtc
    18301 atggctacgc ggcggtgcat cgcggtattc ataccttaag cgcccaagcg accgagaaaa
    18361 tggagaacgt gcgcaagcgg gcatcgctgt ttattaatgc ccgttcggcg gaagagctgg
    18421 tgttcgtccg cggcacgacg gaagggatca atctggtcgc caatagctgg ggtaacagca
    18481 atgtgcgggc gggcgataac atcatcatca gtcagatgga gcaccacgct aacattgttc
    18541 cctggcagat gctttgcgca cgcgttggcg cagagctgcg tgtgatcccg ctcaatcccg
    18601 acggtacgtt gcaactggag acgctgccta cgctgtttga tgagaaaact cgcctgctgg
    18661 caattactca tgtctccaac gtgcttggca cagaaaatcc actggcggaa atgatcacgc
    18721 ttgcgcacca gcatggcgca aaagtgctgg tggatggcgc tcaggcggtg atgcatcatc
    18781 cggtggatgt tcaggcgctg gattgcgact tttacgtgtt ctccgggcat aaactgtatg
    18841 gccccaccgg aattggcatt ctttatgtga aagaagcctt gttgcaggag atgccgccgt
    18901 gggaaggggg cggttctatg atcgccaccg tcagcctgag tgaaggcaca acctggacca
    18961 aagcaccttg gcggtttgaa gccggtacac ccaataccgg gggcatcatt ggtcttggcg
    19021 cggcgctgga gtatgtttcg gcgctggggc ttaataacat agccgagtat gaacagaatc
    19081 tgatgcacta cgcgctatca cagctggaat ctgtaccgga tctcactctc tatggtccgc
    19141 agaacaggct tggcgttatt gcttttaatc ttggtaaaca ccacgcctat gatgttggca
    19201 gttttctcga taactacggc attgctgtgc gtaccggaca tcactgcgct atgccattaa
    19261 tggcctatta caacgtccct gctatgtgtc gggcgtcgct ggccatgtat aacacccatg
    19321 aagaagtgga tcgtctggtg accggactgc aacgtattca ccgtctgctg ggataacagg
    19381 gaggcactat ggctttattg ccggataaag aaaaattgct gcgtaatttt ttacgctgcg
    19441 ccaattggga agagaaatat ctctacatta ttgagctggg ccagcgtctg ccagaattac
    19501 gcgacgaaga cagaagccca caaaatagca ttcagggctg ccagagtcag gtgtggattg
    19561 tcatgcgcca gaatgcgcag ggaattattg aattgcaggg cgacagcgat gcggcgattg
    19621 tgaaagggct tattgcggtc gtctttattc tctacgatca gatgacgccg caggatattg
    19681 tcaatttcga tgtgcgtccg tggtttgaaa aaatggcgct cacccaacat ctcaccccat
    19741 ctcgttcaca aggtctggaa gcgatgattc gcgcaattcg cgccaaagcc gctgcactta
    19801 gctaaactaa agggacagct ttcatccggt tggctcacat tgccggatgg tcaatgttta
    19861 cactgacctg cacaggagtg tttcggtttc aggtgaacat aagcgccatc cgacgtttca
    19921 gcgtgagtct ccggcaaata caggaggttt acaatgaaac gcgcgtcttt gcttacactc
    19981 acgcttatcg gcgcttttag cgccatccag gctgcctggg cggttgatta tccgctacca
    20041 ccaaccggaa gccgactggt tgggcaaaat caaacgtata cggtgcagga aggggataaa
    20101 aaccttcagg ccatcgcccg acgttttgat actgcggcaa tgttgatcct tgaagccaat
    20161 aacactatcg ccccggtgcc aaaacccggt acgacgataa ctattccttc gcaactgtta
    20221 ttacctgatg cgccgcgtca ggggattatc gttaaccttg cagagctgcg cctttattat
    20281 tatccgccgg gagaaaatat cgtgcaggtt tatccgatag gtattggctt gcaggggctg
    20341 gaaacgccgg tgatggaaac gcgtgtgggg cagaaaatcc ctaacccaac ctggacgcct
    20401 actgcaggca ttcgtcaacg ttcgctggag cgtggtatta aattaccgcc agtcgttcct
    20461 gccggaccaa ataacccgct aggacgttac gcactgcgcc tcgcgcatgg taatggcgaa
    20521 tacctcattc atggcaccag tgcgccggac agcgtcggtt tgcgcgtcag ttcagggtgt
    20581 attcgcatga atgcaccgga tattaaagcc ttgttctcca gcgtgcggac gggaacgccg
    20641 gtgaaagtga tcaacgaacc tgtgaaatat tccgtggagc ctaacgggat gcgttatgtt
    20701 gaagtacatc gaccactatc ggcagaagaa cagcagaacg ttcagacaat gccatacaca
    20761 ctgccagcag gctttacgca atttaaagac aataaggctg tagatcagaa gttagtcgat
    20821 aaagcgttgt atcgtcgggc agggtatccg gtttcggtga gcagtggagc aactcccgca
    20881 gccagcaatg cgccttcagt agagtcagcg cagaatggtg aaccagagca agggaatatg
    20941 ttacgcgcga cgcagtagcg gtaaacggca gacaaaaaaa atggcgcaca atgtgcgcca
    21001 tttttcactt cacaggtact attacttgcg gtatttagta gccatgttgt ccagacgctg
    21061 gttagcacga gctgcgtcat ctttagcagc ctgaacgtcg gaacgcattg cgttcacgtc
    21121 gttgctcagc tggtcaactt tagcgttcag agtctgaacg tcagaagaca gctgatcgat
    21181 tttagcgttg ctggagcaac ctgccagcag agtagaaccc aggattaccg cgcccagtac
    21241 cagtttagta gcttt
//
LOCUS       DAZKHF020000063        20714 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2201S31241
            SAMN43267800-rid25106413.denovo.063, whole genome shotgun sequence.
ACCESSION   DAZKHF020000063 DAZKHF020000000
VERSION     DAZKHF020000063.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267800
            Sequence Read Archive: SRR30307263
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 20714)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20714)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 20714)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350581.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350581.2
            Genome Coverage       :: 151x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:21:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,652
            CDSs (total)                      :: 5,557
            Genes (coding)                    :: 5,320
            CDSs (with protein)               :: 5,320
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 237
            CDSs (without protein)            :: 237
            Pseudo Genes (ambiguous residues) :: 0 of 237
            Pseudo Genes (frameshifted)       :: 102 of 237
            Pseudo Genes (incomplete)         :: 130 of 237
            Pseudo Genes (internal stop)      :: 52 of 237
            Pseudo Genes (multiple problems)  :: 41 of 237
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20714
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267800-rid25106413.denovo.063"
                     /serotype="O 26:H11"
                     /isolate="2201S31241"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-01"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            complement(91..858)
                     /gene="ygbI"
                     /locus_tag="ACHOSC_RS09425"
                     /old_locus_tag="pgaptmp_001885"
     CDS             complement(91..858)
                     /gene="ygbI"
                     /locus_tag="ACHOSC_RS09425"
                     /old_locus_tag="pgaptmp_001885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417215.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional repressor YgbI"
                     /protein_id="HGU4547834.1"
                     /translation="MIPVERRQIILEMVAEKGIVSIAELTDRMNVSHMTIRRDLQKLE
                     QQGAVVLVSGGVQSPGRVAHEPSHQVKTALAMTQKAAIGKLAASLVQPGSCIYLDAGT
                     TTLAIAQHLIHMESLTVVTNDFVIANYLLDNSNCTIIHTGGAVCRENRSCVGEAAATM
                     LRSLMIDQAFISASSWSVRGISTPAEDKVTVKRAIASASRQRVLVCDATKYGQVATWL
                     ALPLSEFDQIITDDGLPESASRALAKQDLSLLVAKNE"
     gene            1054..1962
                     /gene="ltnD"
                     /locus_tag="ACHOSC_RS09430"
                     /old_locus_tag="pgaptmp_001886"
     CDS             1054..1962
                     /gene="ltnD"
                     /locus_tag="ACHOSC_RS09430"
                     /old_locus_tag="pgaptmp_001886"
                     /EC_number="1.1.1.411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonate dehydrogenase"
                     /protein_id="HGU4547835.1"
                     /translation="MKTGSEFHVGIVGLGSMGMGAALSCVRAGLSTWGADLNSNACAT
                     LKEAGACGVSDNAATFAEKLDALLVLVVNAAQVKQVLFGETGVAQHLKPGTAVMVSST
                     IASADAQEIATALAGFDLEMLDAPVSGGAVKAANGEMTVMASGSDIAFERLAPVLEAV
                     AGKVYRIGAEPGLGSTVKIIHQLLAGVHIAAGAEAMALAARAGIPLDVMYDVVTNAAG
                     NSWMFENRMRHVVDGDYTPHSAVDIFVKDLGLVADTAKALHFPLPLASTALNMFTSAS
                     NAGYGKEDDSAVIKIFSGITLPGAKS"
     gene            1959..3221
                     /gene="otnK"
                     /locus_tag="ACHOSC_RS09435"
                     /old_locus_tag="pgaptmp_001887"
     CDS             1959..3221
                     /gene="otnK"
                     /locus_tag="ACHOSC_RS09435"
                     /old_locus_tag="pgaptmp_001887"
                     /EC_number="2.7.1.217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001649192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxo-tetronate kinase"
                     /protein_id="HGU4547836.1"
                     /translation="MIKIGVIADDFTGATDIASFLVENGLPTVQINGVPTGKMPEAID
                     ALVISLKTRSCPVVEATQQSLAALSWLQQQGCKQIYFKYCSTFDSTAKGNIGPVTDAL
                     MDALDTPFTVFSPALPVNGRTVYQGYLFVMNQLLAESGMRHHPVNPMTDSYLPRLVEA
                     QSTGRCGVVSAHVFEQGVDAVRQELARLQQEGYRYAVLDALTEHHLEIQGEALRDAPL
                     VTGGSGLAIGLARQWAQENGNQAREAGHPLAGRGVVLSGSCSQMTNRQVAHYRQIAPA
                     REVDVARCLSTETLAAYAHELAEWVLGQESVLAPLVFATASTDALAAIQQQYGAQKAS
                     QAVETLFSQLAARLAAEGVTRFIVAGGETSGVVTQSLGIKGFHIGPTISPGVPWVNAL
                     DKPVSLALKSGNFGDEAFFSRAQREFLS"
     gene            3218..3856
                     /locus_tag="ACHOSC_RS09440"
                     /old_locus_tag="pgaptmp_001888"
     CDS             3218..3856
                     /locus_tag="ACHOSC_RS09440"
                     /old_locus_tag="pgaptmp_001888"
                     /EC_number="4.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldolase"
                     /protein_id="HGU4547837.1"
                     /translation="MSDFAKVEQSLREEMTRIASSFFQRGYATGSAGNLSLLLPDGNL
                     LATPTGSCLGNLDPQRLSKVAADGEWLSGDKPSKEVLFHLALYRNNPRCKAVVHLHST
                     WSTALSCLQGLDSSNVIRPFTPYVVMRMGNVPLVPYYRPGDKRIAQDLAELAADNQAF
                     LLANHGPVVCGESLQEAANNMEELEETAKLIFILGDRPIRYLTAGEIAELRS"
     gene            3861..4637
                     /locus_tag="ACHOSC_RS09445"
                     /old_locus_tag="pgaptmp_001889"
     CDS             3861..4637
                     /locus_tag="ACHOSC_RS09445"
                     /old_locus_tag="pgaptmp_001889"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HPr family phosphocarrier protein"
                     /protein_id="HGU4547838.1"
                     /translation="MPRFAANLSMMFTEVPFIERFAAARKAGFDAVEFLFPYDYSTLQ
                     IQKQLEQNHLTLALFNTAPGDINAGEWGLSALPGREHEAHADIDLALEYALALNCEQV
                     HVMAGVVPAGEDAERYRAVFIDNLRYAADRFALHGKRILVEALSPDVKPHYLFSSQYQ
                     ALAIVEEVARDNVFIQLDTFHAQKVDGNLTHLIRDYAGKYAHVQIAGLPDRHEPDDGE
                     INYPWLFRLFDEVGYQGWIGCEYKPRGLTEEGLGWFDAWR"
     gene            4726..6090
                     /locus_tag="ACHOSC_RS09450"
                     /old_locus_tag="pgaptmp_001890"
     CDS             4726..6090
                     /locus_tag="ACHOSC_RS09450"
                     /old_locus_tag="pgaptmp_001890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015128 - gluconate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0035429 - gluconate transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntP family transporter"
                     /protein_id="HGU4547839.1"
                     /translation="MSTITLLCIALAGVIMLLLLVIKAKVQPFVALLLVSLLVALAAG
                     IPAGEVGKVMIAGMGGVLGSVTIIIGLGAMLGRMIEHSGGAESLANYFSRKLGDKRTI
                     AALTLAAFFLGIPVFFDVGFIILAPIIYGFAKVAKISPLKFGLPVAGIMLTVHVAVPP
                     HPGPVAAAGLLHADIGWLTIIGIAISIPVGIVGYFAAKIINTRQYAMSVEVLEQMQLA
                     PASEEGATKLSDKINPPGVALVTSLIVIPIAIIMAGTVSATLMPPSHPLLGTLQLIGS
                     PMVALMIALVLAFWLLALRRGWSLQHTSDIMGSALPTAAVVILVTGAGGVFGKVLVES
                     GVGKALANMLQMIDLPLLPAAFIISLALRASQGSATVAILTTGGLLSEAVMGLNPIQC
                     VLVTLAACFGGLGASHINDSGFWIVTKYLGLSVADGLKTWTVLTTILGFTGFLITWCV
                     WAVI"
     gene            complement(6129..6365)
                     /locus_tag="ACHOSC_RS09455"
                     /old_locus_tag="pgaptmp_001891"
     CDS             complement(6129..6365)
                     /locus_tag="ACHOSC_RS09455"
                     /old_locus_tag="pgaptmp_001891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="non-oxidative hydroxyarylic acid decarboxylases
                     subunit D"
                     /protein_id="HGU4547840.1"
                     /translation="MICPRCADEQIEVMAKSPVKDVWTVYQCQHCLYTWRDTESLRRT
                     SREHYPEAFRMTQKDIDDAPMVPSIPPLLVEGKR"
     gene            complement(6376..7803)
                     /locus_tag="ACHOSC_RS09460"
                     /old_locus_tag="pgaptmp_001892"
     CDS             complement(6376..7803)
                     /locus_tag="ACHOSC_RS09460"
                     /old_locus_tag="pgaptmp_001892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016831 - carboxy-lyase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="non-oxidative hydroxyarylic acid decarboxylases
                     subunit C"
                     /protein_id="HGU4547841.1"
                     /translation="MAFDDLRSFLQALDDHGQLLKISEEVNAEPDLAAAANATGRIGD
                     GAPALWFDNIRGFTDARVAMNTIGSWQNHAISLGLPPNAPVKKQIDEFIRRWDNFPIA
                     PERRANPAWAQNTVDGDEINLFDILPLFRLNDGDGGFYLDKACVVSRDPLDPDNFGKQ
                     NVGIYRMEVKGKRKLGLQPVPMHDIALHLHKAEERGEDLPIAITLGNDPIITLMGATP
                     LKYDQSEYEMAGALRESPYPIATAPLTGFDVPWGSEVILEGVIESRKREIEGPFGEFT
                     GHYSGGRNMTVVRIDKVSYRTRPIFESLYLGMPWTEIDYLMGPATCVPLYQQLKAEFP
                     EVQAVNAMYTHGLLAIISTKKRYGGFARAVGLRAMTTPHGLGYVKMVIMVDEDVDPFN
                     LPQVMWALSSKVNPAGDLVQLPNMSVLELDPGSSPAGITDKLIIDATTPVAPDNRGHY
                     SQPVVDLPETKAWAEKLTAMLAARK"
     gene            complement(7803..8396)
                     /locus_tag="ACHOSC_RS09465"
                     /old_locus_tag="pgaptmp_001893"
     CDS             complement(7803..8396)
                     /locus_tag="ACHOSC_RS09465"
                     /old_locus_tag="pgaptmp_001893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="non-oxidative hydroxyarylic acid decarboxylases
                     subunit B"
                     /protein_id="HGU4547842.1"
                     /translation="MKLIVGMTGATGAPLGVALLQALREMPNVETHLVMSKWAKTTIE
                     LETPYSARDVAALADFSHNPADQAAIISSGSFRTDGMIVIPCSMKTLAGIRAGYADGL
                     VGRAADVVLKEGRKLVLVPREMPLSTIHLENMLALSRMGVAMVPPMPAFYNHPETVDD
                     IVHHVVARVLDQFGLEHPHARRWQGLPQARNFSQENE"
     gene            8543..8950
                     /locus_tag="ACHOSC_RS09470"
                     /old_locus_tag="pgaptmp_001894"
     CDS             8543..8950
                     /locus_tag="ACHOSC_RS09470"
                     /old_locus_tag="pgaptmp_001894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001208089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family winged helix-turn-helix
                     transcriptional regulator"
                     /protein_id="HGU4547843.1"
                     /translation="MALRNKAFHQLRQLFQQHTARWQHELPDLTKPQYAVMRAIADKP
                     GIEQVALIEAAVSTKATLAEMLARMENRGLVRREHDPADKRRRFVWLTAEGEKILAAA
                     IPIGDSVDEEFLGRLSGEEQELFMQLVRKMMNA"
     gene            complement(9071..10063)
                     /gene="rpoS"
                     /locus_tag="ACHOSC_RS09475"
                     /old_locus_tag="pgaptmp_001895"
     CDS             complement(9071..10063)
                     /gene="rpoS"
                     /locus_tag="ACHOSC_RS09475"
                     /old_locus_tag="pgaptmp_001895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000081498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoS"
                     /protein_id="HGU4547844.1"
                     /translation="MSQNTLKVHDLNEDAEFDENGVEVFDEKALVEEEPSDNDLAEEE
                     LLSQGATQRVLDATQLYLGEIGYSPLLTAEEEVYFARRALRGDVASRRRMIESNLRLV
                     VKIARRYGNRGLALLDLIEEGNLGLIRAVEKFDPERGFRFSTYATWWIRQTIERAIMN
                     QTRTIRLPIHIVKELNVYLRTARELSHKLDHEPSAEEIAEQLDKPVDDVSRMLRLNER
                     ITSVDTPLGGDSEKALLDILADEKENGPEDTTQDDDMKQSIVKWLFELNAKQREVLAR
                     RFGLLGYEAATLEDVGREIGLTRERVRQIQVEGLRRLREILQTQGLNIEALFRE"
     gene            complement(10126..11220)
                     /gene="nlpD"
                     /locus_tag="ACHOSC_RS09480"
                     /old_locus_tag="pgaptmp_001896"
     CDS             complement(10126..11220)
                     /gene="nlpD"
                     /locus_tag="ACHOSC_RS09480"
                     /old_locus_tag="pgaptmp_001896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein hydrolase activator NlpD"
                     /protein_id="HGU4547845.1"
                     /translation="MSLVSLWLAGCSDTSNPPAPVSSVNGNAPANTNSGMLITPPPKM
                     GTTSTAQQPQIQPVQQPQIQATQQPQIQPVQPVAQQPVQMENGRIVYNRQYGNIPKGS
                     YSGSTYTVKKGDTLFYIAWITGNDFRDLAQRNNIQAPYALNVGQTLQVGNASGTPITG
                     GNAITQADAAEQGVVIKPAQNSTVAVASQPTITYSESSGEQSANKMLPNNKPAATTVT
                     APVTVPTASTTEPTVSSTSTSTPISTWRWPTEGKVIETFGASEGGNKGIDIAGSKGQA
                     IIATADGRVVYAGNALRGYGNLIIIKHNDDYLSAYAHNDTMLVREQQEVKAGQKIATM
                     GSTGTSSTRLHFEIRYKGKSVNPLRYLPQR"
     gene            complement(11405..12031)
                     /gene="pcm"
                     /locus_tag="ACHOSC_RS09485"
                     /old_locus_tag="pgaptmp_001897"
     CDS             complement(11405..12031)
                     /gene="pcm"
                     /locus_tag="ACHOSC_RS09485"
                     /old_locus_tag="pgaptmp_001897"
                     /EC_number="2.1.1.77"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005130872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004719 - protein-L-isoaspartate
                     (D-aspartate) O-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein-L-isoaspartate O-methyltransferase"
                     /protein_id="HGU4547846.1"
                     /translation="MVSRRVQALLDQLRAQGIQDEQVLNALAAVPREKFVDEAFEQKA
                     WDNIALPIGQGQTISQPYMVARMTELLELTPQSRVLEIGTGSGYQTAILAHLVQHVCS
                     VERIKGLQWQARRRLKNLDLHNVSTRHGDGWQGWQARAPFDAIIVTAAPPEIPTALMT
                     QLDEGGILVLPVGEEHQYLKRVRRRGGEFIIDTVEAVRFVPLVKGELA"
     gene            complement(12025..12786)
                     /gene="surE"
                     /locus_tag="ACHOSC_RS09490"
                     /old_locus_tag="pgaptmp_001898"
     CDS             complement(12025..12786)
                     /gene="surE"
                     /locus_tag="ACHOSC_RS09490"
                     /old_locus_tag="pgaptmp_001898"
                     /EC_number="3.1.3.5"
                     /EC_number="3.1.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008252 - nucleotidase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5'/3'-nucleotidase SurE"
                     /protein_id="HGU4547847.1"
                     /translation="MRILLSNDDGVHAPGIQTLAKALREFADVQVVAPDRNRSGASNS
                     LTLESSLRTFTFENGDIAVQMGTPTDCVYLGVNALMRPRPDIVVSGINAGPNLGDDVI
                     YSGTVAAAMEGRHLGFPALAVSLDGHKHYDTAAAVTCSILRALCKEPLRTGRILNINV
                     PDLPLDQIKGIRVTRCGTRHPADQVIPQQDPRGNTLYWIGPPGGKCDAGPGTDFAAVD
                     EGYVSITPLHVDLTAHSAQDVVSDWLNSVGVGTQW"
     gene            complement(12767..13816)
                     /gene="truD"
                     /locus_tag="ACHOSC_RS09495"
                     /old_locus_tag="pgaptmp_001899"
     CDS             complement(12767..13816)
                     /gene="truD"
                     /locus_tag="ACHOSC_RS09495"
                     /old_locus_tag="pgaptmp_001899"
                     /EC_number="5.4.99.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(13) synthase TruD"
                     /protein_id="HGU4547848.1"
                     /translation="MIEFDNLTYLHGKPQGTGLLKANPEDFVVVEDLGFEPDGEGEHI
                     LVRILKNGCNTRFVADALAKFLKIHAREVSFAGQKDKHAVTEQWLCARVPGKEMPDLS
                     AFQLEGCQVLEYARHKRKLRLGALKGNAFTLVLREVSNRDDVEQRLNDICVKGVPNYF
                     GAQRFGIGGSNLQGAQRWAQTNTPVRDRNKRSFWLSAARSALFNQIVAERLKKADVNQ
                     VVDGDALQLAGRGSWFVATTEELAELQRRVNDKELMITAALPGSGEWGTQREALAFEQ
                     AAVAAETELQALLVREKVEAARRAMLLYPQQLSWNWWDDVTVEIRFWLPAGSFATSVV
                     RELINTTGDYAHIAE"
     gene            complement(13813..14292)
                     /gene="ispF"
                     /locus_tag="ACHOSC_RS09500"
                     /old_locus_tag="pgaptmp_001900"
     CDS             complement(13813..14292)
                     /gene="ispF"
                     /locus_tag="ACHOSC_RS09500"
                     /old_locus_tag="pgaptmp_001900"
                     /EC_number="4.6.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008685 - 2-C-methyl-D-erythritol
                     2,4-cyclodiphosphate synthase activity [Evidence IEA];
                     GO_process: GO:0019288 - isopentenyl diphosphate
                     biosynthetic process, methylerythritol 4-phosphate pathway
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-C-methyl-D-erythritol 2,4-cyclodiphosphate
                     synthase"
                     /protein_id="HGU4547849.1"
                     /translation="MRIGHGFDVHAFGGEGPIIIGGVRIPYEKGLLAHSDGDVALHAL
                     TDALLGAAALGDIGKLFPDTDPAFKGADSRELLREAWRRIQAKGYTLGNVDVTIIAQA
                     PKMLPHIPQMRVFIAEDLGCHMDDVNVKATTTEKLGFTGRGEGIACEAVALLIKATK"
     gene            complement(14292..15002)
                     /gene="ispD"
                     /locus_tag="ACHOSC_RS09505"
                     /old_locus_tag="pgaptmp_001901"
     CDS             complement(14292..15002)
                     /gene="ispD"
                     /locus_tag="ACHOSC_RS09505"
                     /old_locus_tag="pgaptmp_001901"
                     /EC_number="2.7.7.60"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050518 - 2-C-methyl-D-erythritol
                     4-phosphate cytidylyltransferase activity [Evidence IEA];
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019288 - isopentenyl diphosphate
                     biosynthetic process, methylerythritol 4-phosphate pathway
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-C-methyl-D-erythritol 4-phosphate
                     cytidylyltransferase"
                     /protein_id="HGU4547850.1"
                     /translation="MATTHLDVCAVVPAAGFGRRMQTECPKQYLSIGNQTILEHSVHA
                     LLAHPRVKRVVIAISPGDSRFAQLPLANHPQITVVDGGDERADSVLAGLKAAGDAQWV
                     LVHDAARPCLHQDDLARLLALSETSRTGGILAAPVRDTMKRAEPGKNAIAHTVDRNGL
                     WHALTPQFFPRELLHDCLTRALNEGATITDEASALEYCGFHPQLVEGRADNIKVTRPE
                     DLALAEFYLTRTIHQENT"
     gene            complement(15021..15332)
                     /gene="ftsB"
                     /locus_tag="ACHOSC_RS09510"
                     /old_locus_tag="pgaptmp_001902"
     CDS             complement(15021..15332)
                     /gene="ftsB"
                     /locus_tag="ACHOSC_RS09510"
                     /old_locus_tag="pgaptmp_001902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsB"
                     /protein_id="HGU4547851.1"
                     /translation="MGKLTLLLLAILVWLQYSLWFGKNGIHDYTRVNDDVAAQQATNA
                     KLKARNDQLFAEIDDLNGGQEALEERARNELSMTRPGETFYRLVPDASKRAQSAGQNN
                     R"
     gene            complement(15526..15849)
                     /locus_tag="ACHOSC_RS09515"
                     /old_locus_tag="pgaptmp_001903"
     CDS             complement(15526..15849)
                     /locus_tag="ACHOSC_RS09515"
                     /old_locus_tag="pgaptmp_001903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3561 family protein"
                     /protein_id="HGU4547852.1"
                     /translation="MRNSHNITLTNNDSLTEDEETTWSLPGAVVGFISWLFALAMPML
                     IYGSNTLFFFIYTWPFFLALMPVAVVVGIALHSLMDGKLRYSIVFTLVTVGIMFGALF
                     MWLLG"
     gene            complement(15899..16504)
                     /gene="cysC"
                     /locus_tag="ACHOSC_RS09520"
                     /old_locus_tag="pgaptmp_001904"
     CDS             complement(15899..16504)
                     /gene="cysC"
                     /locus_tag="ACHOSC_RS09520"
                     /old_locus_tag="pgaptmp_001904"
                     /EC_number="2.7.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004020 - adenylylsulfate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0000103 - sulfate assimilation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylyl-sulfate kinase"
                     /protein_id="HGU4547853.1"
                     /translation="MALHDENVVWHSHPVTVQQRELHHGHRGVVLWFTGLSGSGKSTV
                     AGALEEALHKLGVSTYLLDGDNVRHGLCSDLGFSDADRKENIRRVGEVANLMVEAGLV
                     VLTAFISPHRAERQMVRERVGEGRFIEVFVDTPLAICEARDPKGLYKKARAGELRNFT
                     GIDSVYEAPESAEIHLNGEQLVTNLVQQLLDLLRQNDIIRS"
     gene            complement(16504..17931)
                     /gene="cysN"
                     /locus_tag="ACHOSC_RS09525"
                     /old_locus_tag="pgaptmp_001905"
     CDS             complement(16504..17931)
                     /gene="cysN"
                     /locus_tag="ACHOSC_RS09525"
                     /old_locus_tag="pgaptmp_001905"
                     /EC_number="2.7.7.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006790 - sulfur compound metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate adenylyltransferase subunit CysN"
                     /protein_id="HGU4547854.1"
                     /translation="MNTALAQQIANEGGVEAWMIAQQHKSLLRFLTCGSVDDGKSTLI
                     GRLLHDTRQIYEDQLSSLHNDSKRHGTQGEKLDLALLVDGLQAEREQGITIDVAYRYF
                     STEKRKFIIADTPGHEQYTRNMATGASTCELAILLIDARKGVLDQTRRHSFISTLLGS
                     KHLVVAINKMDLVDYSEETFTRIREDYLTFAGQLPGNLDIRFVPLSALEGDNVASQSE
                     SMPWYSGPTLLEVLETVEIQRVVDAQPMRFPVQYVNRPNLDFRGYAGTLASGRVEVGQ
                     RVKVLPSGVESNVARIVTFDGDREEAFAGEAITLVLTDEIDISRGDLLLAADEALPAV
                     QSASVDVVWMAEQPLSPGQSYDIKIAGKKTRARVDGIRYQVDINNLTQREVENLPLNG
                     IGLVDLTFDEPLVLDRYQQNPVTGGLIFIDRLSNVTVGAGMVHEPVSQATAAPSEFSA
                     FELELNALVRRHFPHWGARDLLGDK"
     gene            complement(17933..18841)
                     /gene="cysD"
                     /locus_tag="ACHOSC_RS09530"
                     /old_locus_tag="pgaptmp_001906"
     CDS             complement(17933..18841)
                     /gene="cysD"
                     /locus_tag="ACHOSC_RS09530"
                     /old_locus_tag="pgaptmp_001906"
                     /EC_number="2.7.7.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005130891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004781 - sulfate adenylyltransferase
                     (ATP) activity [Evidence IEA];
                     GO_process: GO:0000103 - sulfate assimilation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate adenylyltransferase subunit CysD"
                     /protein_id="HGU4547855.1"
                     /translation="MDQIRLTHLRQLEAESIHIIREVAAEFSNPVMLYSIGKDSSVML
                     HLARKAFYPGTLPFPLLHVDTGWKFREMYEFRDRTAKAYGCELLVHKNPEGVAMGINP
                     FVHGSAKHTDIMKTEGLKQALNKYGFDAAFGGARRDEEKSRAKERIYSFRDRFHRWDP
                     KNQRPELWHNYNGQINKGESIRVFPLSNWTEQDIWQYIWLENIDIVPLYLAAERPVLE
                     RDGMLMMIDDNRIDLQPGEVIKKRMVRFRTLGCWPLTGAVESNAQTLPEIIEEMLVST
                     TSERQGRVIDRDQAGSMELKKRQGYF"
     gene            19093..20130
                     /gene="iap"
                     /locus_tag="ACHOSC_RS09535"
                     /old_locus_tag="pgaptmp_001907"
     CDS             19093..20130
                     /gene="iap"
                     /locus_tag="ACHOSC_RS09535"
                     /old_locus_tag="pgaptmp_001907"
                     /EC_number="3.4.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase isozyme conversion
                     aminopeptidase"
                     /protein_id="HGU4547856.1"
                     /translation="MFSALRHRTAALALGVCFILPVHASSPKPGDFANTQARHIATFF
                     PGRMTGTPAEMLSADYIRQQFQQMGYRSDIRTFNSRYIYTARDNRKSWHNVTGSTVIA
                     AHEGKAPQQIIIMAHLDTYAPLSDADADANLGGLTLQGMDDNAAGLGVMLELAERLKN
                     TPTEYGIRFVATSGEEEGKLGAENLLKRMSDTEKKNTLLVINLDNLIVGDKLYFNSGV
                     KTPEAVRKLTRDRALAIARSHGIAATTNPGLNKNYPKGTGCCNDAEIFDKAGIAVLSV
                     EATNWNLGNKDGYQQRAKTPAFPAGNSWHDVRLDNQQHIDKALPGRIERRCRDVMRIM
                     LPLVKELAKAS"
     repeat_region   20151..20667
                     /inference="COORDINATES: alignment:crt:1.2"
                     /rpt_family="CRISPR"
                     /rpt_type=direct
                     /rpt_unit_range=20273..20301
                     /rpt_unit_seq="cggtttatccccgctggcgcggggaacac"
BASE COUNT         4799 a         5614 c         5325 g         4976 t
ORIGIN      
        1 gccgaaaagc gggcggctgt cattttataa aataaagtag tctcatgctt cttctgtgaa
       61 gcatgagtaa ccaaatgtta ttgcaggcca ttattcattt ttcgctacca gcaaagagag
      121 atcctgcttc gccagcgcgc gactggcact ctccggcaaa ccgtcgtctg taataatctg
      181 atcaaactcg cttaatggta acgccagcca tgtcgccacc tgaccatatt tcgtcgcatc
      241 acagaccaaa actcgctggc ggctggcact ggcaatcgcc cgtttcaccg tgactttatc
      301 ttccgctggc gtagaaatcc cccgcacact ccatgacgat gcagaaataa aagcctgatc
      361 aatcatcagg ctgcgcagca tggtcgcagc ggcttccccg acacaggaac ggttttcccg
      421 acacactgca ccgccagtgt gaataattgt gcaattactg ttgtcgagca agtagttcgc
      481 aataacgaaa tcgtttgtga ccacagtcag tgactccatg tgaatcagat gctgtgctat
      541 cgctaacgtg gtcgttcccg catccagata gatacaactt cccggctgaa caagacttgc
      601 cgccagcttg ccaatagccg ctttttgcgt cattgccagc gcagttttta cctgatgaga
      661 aggttcatgc gccacgcgtc ccggagactg gacgcctccg gacaccagca caacggctcc
      721 ctgctgctcc agtttttgta aatcccgacg aatggtcata tgtgacacat tcattctgtc
      781 cgttagttca gcaatactga caatgccttt ttcagctacc atctcaagga tgatttggcg
      841 acgctctacg ggtatcaact tttgctcctt cctttgtcct gctgacattc tacgctattt
      901 gcctgcgaaa cgtgcgcggc gcaactaacg cttagttcac ataaaataac acacaatgtt
      961 aatttatgtg aatcagctca ccatatcgtt atcttccagc gcttatattc acaatatcaa
     1021 acaaaatatc acttaaatta acaaggggag cagatgaaaa cgggatctga gtttcatgtc
     1081 ggtatcgttg gcttagggtc aatgggaatg ggagcagcac tgtcatgtgt ccgcgcaggt
     1141 ctttctacct ggggcgcaga cctgaacagc aatgcctgcg ctacgttgaa agaggcaggt
     1201 gcttgcgggg tttctgataa cgccgcgacg tttgccgaaa aactggacgc tctgctggtg
     1261 ctggtggtca atgcggccca ggttaaacag gtgctgtttg gtgaaacagg cgttgcacaa
     1321 catctgaaac ccggtacggc agtaatggtt tcttccacta tcgctagtgc tgatgcgcaa
     1381 gaaattgcta ccgctctggc tggattcgat ctggaaatgc tggatgcgcc agtttctggt
     1441 ggtgcagtaa aagccgctaa cggtgaaatg actgtcatgg cctccggtag cgatattgcc
     1501 tttgaacgac tggcacccgt gctggaagcc gttgccggaa aagtttatcg cataggtgca
     1561 gaaccgggac taggttcgac cgtaaaaatt attcaccagt tgttagcggg cgtacatatt
     1621 gctgccggag ccgaagcgat ggcacttgca gcccgtgcgg ggatcccgct ggatgtgatg
     1681 tatgacgtcg tgaccaatgc cgccggaaat tcctggatgt tcgaaaaccg gatgcgtcat
     1741 gtggtggatg gcgattacac cccgcattca gccgtcgata tttttgttaa ggatcttggt
     1801 ctggttgccg atacagccaa agccctgcac ttcccgctgc cattggcctc aacagcattg
     1861 aatatgttca ccagcgccag taacgcaggt tacgggaaag aagacgatag cgcagttatc
     1921 aagattttct ctggcatcac tctaccggga gcgaaatcat gatcaagatt ggcgttatcg
     1981 ccgatgattt taccggcgcg acggatatcg ccagttttct ggtggaaaac ggtctaccaa
     2041 cggtacaaat taacggtgtt ccaacaggta aaatgccgga agcaatcgac gcactggtga
     2101 tcagcctgaa aacgcgctcc tgtccggtgg ttgaagccac acagcaatcg ctggcggctc
     2161 tgagctggtt gcaacagcaa ggttgcaaac agatctattt caaatactgc tctactttcg
     2221 acagtacggc gaaaggtaat atcggcccgg ttaccgatgc attaatggat gctctcgaca
     2281 cgccgtttac ggtcttctct ccggccctgc cggtcaacgg acgtacggtt tatcaggggt
     2341 atttgttcgt aatgaatcaa ctgctggccg aatccgggat gcgccatcat ccggtaaatc
     2401 ccatgaccga cagctatctt ccccgtctgg ttgaagcgca atccacaggg cgctgcggcg
     2461 tcgtttcggc acatgttttc gaacaaggtg tggatgccgt tcgtcaagag ctggctcgct
     2521 tacagcaaga gggctaccgc tacgcggtgc ttgatgcgct gaccgaacac catctggaaa
     2581 ttcagggaga agccttgcgc gatgccccac tggtaacggg cggttctggt ctggcgattg
     2641 gcctggcccg gcagtgggcg caagaaaacg gtaaccaggc tcgcgaagca gggcatccgc
     2701 tcgctgggcg cggcgtagtg ctctccggtt catgctctca aatgaccaac cgccaggtgg
     2761 cacattaccg tcaaattgca ccagcccgtg aagttgatgt ggcacgctgc ctctcaaccg
     2821 aaactctggc cgcttatgca cacgaactgg cagagtgggt tctgggccag gaaagtgtac
     2881 ttgctccact ggtttttgcc accgccagca ctgacgcatt ggcagcaatt caacagcaat
     2941 acggtgcaca aaaagccagt caggcagtag aaactctatt ttctcaacta gcggcgcggt
     3001 tagcagcgga aggcgtgaca cgctttattg tcgcaggcgg tgagacctcc ggcgtagtca
     3061 cacagagcct gggcataaaa gggtttcata ttggcccaac catttccccc ggcgtgccgt
     3121 gggtaaacgc actggataag cctgtctcac tcgcccttaa atctggcaac ttcggtgatg
     3181 aagccttttt ttcacgagcc caaagagagt ttttatcatg agcgatttcg caaaagtaga
     3241 gcagtctttg cgagaggaga tgacgcggat tgccagttca ttctttcagc gcggctatgc
     3301 aaccggttcg gctggcaatc tgtcgctgct tttacctgac gggaatttac tggcgacacc
     3361 gacaggttca tgcctgggca atctcgatcc gcagcggctt tccaaagtcg ccgcggatgg
     3421 cgaatggtta agtggtgaca aaccctcgaa agaggtgctc tttcatctgg cgctgtatcg
     3481 caacaatccg cgctgtaaag cggtggtgca tttgcacagc acatggtcga cggcgctttc
     3541 ctgcctgcaa gggctggaca gcagcaacgt tattcgtccg ttcacaccat acgtggtgat
     3601 gcggatggga aatgtcccgc tggtgcctta ttaccgaccg ggcgataaac gcatcgcaca
     3661 ggatctggcg gaactggcag cagacaatca ggctttttta ctggcaaatc atggcccagt
     3721 ggtttgcggt gaaagcctgc aagaagccgc caacaatatg gaagagctgg aggaaacggc
     3781 aaagctgatt tttattctcg gtgaccgccc gatccgttat ctgaccgcag gtgaaattgc
     3841 ggaattaagg agttaatgca atgcctcgtt ttgcagctaa tttatccatg atgttcaccg
     3901 aagtgccttt tattgaacgc ttcgccgcag cgcgaaaagc cgggttcgat gctgtggaat
     3961 ttctgtttcc ctatgactac tccaccctgc aaatccaaaa gcaactggag caaaaccatc
     4021 tgacactggc gctgttcaat accgctcccg gagatattaa tgcgggggaa tggggattat
     4081 ccgcccttcc cggacgtgag cacgaagcac acgcagacat cgacctggca ctggaatatg
     4141 cattggcgct caattgtgaa caagtccatg tgatggcagg cgtcgtgccc gctggcgaag
     4201 acgctgaacg gtaccgggca gtatttatcg ataatctccg ctacgctgcc gaccgctttg
     4261 cgctacacgg caagcgaata ttagttgaag cactcagccc cgacgttaag cctcattatc
     4321 tcttctccag ccagtatcag gcactggcta ttgttgaaga ggttgcgcga gataacgtgt
     4381 ttattcaact agatactttt catgcgcaaa aagtagatgg caacctgacg catttaatcc
     4441 gcgactacgc tgggaaatat gcgcacgtac aaattgccgg actacctgat cgacatgaac
     4501 cggacgatgg agaaatcaac tatccgtggc tgttccgcct gttcgatgag gtgggatatc
     4561 agggctggat cggttgtgaa tataaacctc gtggcctcac cgaagaaggg cttggctggt
     4621 ttgacgcctg gcgctaattc gttaaatccc taattacaac gtacccatac atccccccat
     4681 ctacggatgg ggtaggttaa ctataacttt cagacagggt ttcccatgtc cacaattaca
     4741 ttgttatgca ttgcgttagc tggcgtaatc atgctgttgc tgctggtcat caaggcaaag
     4801 gtacaaccat tcgttgctct gctcctcgtc agcctgttag tcgcacttgc ggcgggtata
     4861 ccggcgggcg aagtgggtaa agtgatgatc gccgggatgg gcggtgtgct tggttccgtc
     4921 actattatta ttggtctggg cgctatgctg gggcgtatga tcgaacactc tggtggtgca
     4981 gagtcactgg ctaattattt cagtcgcaag ttaggtgaca aacgaactat cgctgcgctg
     5041 actctggcag cgttcttcct cggtattccc gtcttctttg atgtcggctt tattattctt
     5101 gcgccaatca tttacggttt tgccaaggtt gccaaaatat cgccactcaa atttggcctg
     5161 cctgtcgctg gaatcatgct aactgttcat gtggcggtac cgccgcatcc aggccctgtc
     5221 gccgcagcgg ggttactcca cgcagacatc ggctggctaa ccatcatcgg tattgcgatt
     5281 tctattcccg tagggattgt tggctacttt gcagcgaaaa taatcaatac gcgccaatat
     5341 gcgatgtcgg tggaagtact ggaacagatg caactggctc cggccagtga ggaaggcgca
     5401 acaaaattaa gcgataaaat aaatccaccg ggcgtcgcgc tggtcacctc gctaattgtt
     5461 attcctatcg cgattatcat ggcgggtacg gtttccgcaa cactgatgcc gccttcgcat
     5521 cccctgcttg gtacgctaca gctgatcggc tcaccaatgg tagctctaat gattgcgctg
     5581 gtgctggcat tctggttatt ggctttacgt cgcggctgga gcttacaaca caccagcgac
     5641 attatgggct cagcccttcc cactgcggcg gtagtgattt tggttactgg tgctggaggg
     5701 gtatttggca aagtgctggt ggaatcgggc gtcggcaaag cccttgccaa tatgctacaa
     5761 atgattgacc tgcctctgtt accagccgca tttattattt cactggctct gcgtgcatcg
     5821 caggggtcag caaccgtagc aatcctgaca accggcgggt tactctcaga agcggtgatg
     5881 ggattgaatc cgattcagtg cgtattggtg acgctggcag cctgctttgg tgggcttggc
     5941 gcgtcacata ttaatgactc agggttctgg attgtgacca aatatctggg gttgtcggta
     6001 gcagacggtc tgaaaacctg gacagtgtta acgaccattc tcggttttac cggattctta
     6061 attacctggt gcgtatgggc ggtaatttga ccttaataaa aaggtccgat gggcatcgga
     6121 ccttttattt aacgcttacc ttccaccagc agcggcggga tgctcggcac cattggcgcg
     6181 tcatcaatat ctttctgcgt catgcggaac gcttcgggat aatgttcgcg gctggtacgg
     6241 cgcagcgatt cggtatcgcg ccaggtataa aggcaatgct ggcactgata taccgtccag
     6301 acatctttca ccggcgattt cgccatcact tcaatctgtt catcggcaca acgtggacaa
     6361 atcatcttct tctccttatt tacgtgcagc cagcatagcg gtcagttttt cagcccaggc
     6421 tttggtttcc ggtaaatcca ccaccggttg gctgtagtga ccacggttgt ccggggcgac
     6481 aggcgtagtg gcgtcgataa tcagcttgtc ggtgatcccc gcagggcttg agcctggatc
     6541 gagttccagc acggacatat tcggcaactg caccaaatcc cctgccgggt tcactttcga
     6601 ggagagcgcc cacatcacct gcggcaggtt gaacgggtca acgtcttcat cgaccataat
     6661 caccatcttc acgtagccca gaccatgcgg cgtggtcatt gcgcgcaggc ccaccgcgcg
     6721 ggcaaagccg ccgtagcgtt ttttggtgga gataatcgcc agcaggccat gggtgtacat
     6781 ggcgtttacc gcctgcactt cagggaactc ggctttcagc tgctgataca gcggcacgca
     6841 ggtggctggc cccatcaggt agtcgatttc ggtccacggc ataccgaggt acagcgattc
     6901 gaaaatcggc ctggtgcggt aagagacttt atcgatgcgc accacggtca tgttacgccc
     6961 gccggagtag tgcccggtaa actcaccgaa cggcccttcg atttcgcgtt tacggctttc
     7021 gatgacccct tcgaggatca cttctgaacc ccacggcaca tcaaaaccgg tcaacggggc
     7081 ggtggcgatc gggtacgggc tttcacgcag cgcgcctgcc atttcgtact cggactgatc
     7141 atatttcagc ggcgtggccc ccatcagcgt gatgatcgga tcgttaccga gcgtgatcgc
     7201 aatcggcaga tcttcaccgc gctcttctgc tttatgcaga tgcagggcga tatcgtgcat
     7261 cggcaccggt tgcaggccga gcttacgctt gcccttcact tccatgcggt agatgccgac
     7321 gttctgcttg ccgaagttat ccgggtcgag cggatcgcgg gaaaccacgc acgctttgtc
     7381 gagatagaaa ccgccatcgc catcgtttaa acgaaacagc ggcaggatat cgaacaggtt
     7441 gatctcgtcg ccatcaacgg tgttctgcgc ccaggctgga ttggcgcggc gctccggggc
     7501 aatcgggaag ttatcccagc ggcggataaa ctcatcaatc tgctttttaa ccggggcatt
     7561 tggcggcagg ccgagggaaa tcgcgtggtt ctgccaggaa ccgatggtgt tcatcgccac
     7621 gcgggcatcg gtaaagccgc gaatattatc aaaccacagc gcgggcgcgc cgtcgccgat
     7681 acgcccggtg gcgttagctg ctgctgccag atccggctcg gcgttcactt cttcgctgat
     7741 tttcagtaac tggccgtggt catcaagcgc ctgtaaaaag ctgcgtaaat catcaaatgc
     7801 cattattcat tctcctgaga aaaattccgg gcctgcggca atccttgcca gcgcctggcg
     7861 tggggatgtt cgaggccaaa ttgatccagc acgcgggcta ccacatggtg gacaatgtca
     7921 tctaccgttt cgggatggtt ataaaaggca ggcatcggcg gcaccatcgc cacgcccatg
     7981 cgtgaaagtg cgagcatatt ttcgagatgg atggtgctaa gcggcatttc acgcggcacc
     8041 agcaccagtt tgcggccttc tttgagcacg acgtccgccg cgcgccctac caggccatcg
     8101 gcgtaaccag cgcggatacc ggcgagcgtt ttcatactgc acggaataac gatcatgccg
     8161 tcggtacgaa aagaaccgga tgagatgatc gccgcctgat ccgccgggtt atggctgaag
     8221 tctgcgaggg cagcaacatc gcgagcgctg taaggcgttt ccagttcaat ggtggttttc
     8281 gcccacttcg acatcaccag atgagtctcg acattcggca tctcccgcag cgcttgcagt
     8341 aatgccacac caagaggcgc accggtagcc cctgtcatcc cgacgatcag tttcattgct
     8401 tatcctctta attgttcgta tacgaacgtt attattaaca tactccttca tttccacagc
     8461 ggcaaggatt gaaaagtaac aacatcttct tcgtcactaa aaaaccgcta tgatagcgac
     8521 agagaattag cccggagttt acatggcgtt acgaaataaa gcgttccatc agttacgaca
     8581 gctttttcag cagcacacgg ctcgctggca gcacgagtta cctgacctga ccaaaccaca
     8641 gtacgcggtg atgcgcgcca ttgctgataa acctggtatc gaacaggtgg cactgataga
     8701 agcagctgtc agcaccaaag caacgttagc agaaatgttg gcaagaatgg aaaatcgtgg
     8761 cttagtcaga cgtgaacatg accccgctga taagcggcga cgctttgtct ggttgactgc
     8821 cgaaggcgaa aagatacttg ctgcggcgat acccattggt gacagcgtgg atgaggaatt
     8881 tttggggcgt ttgagtggag aagagcagga gctgtttatg cagctggtgc gcaagatgat
     8941 gaacgcatag ggggcaaatt tccggatgcg acgctatcgc gtcttatcca gactacaaat
     9001 acaccgactc accaaccgaa agaaaaaagg ccagcctcgc ttgagactgg cctttctgac
     9061 agatgcttac ttactcgcgg aacagcgctt cgatattcag cccctgcgtt tgcaggattt
     9121 cgcgcaaacg gcgcaggcct tcaacctgaa tctggcgaac acgttcacgg gtgaggccaa
     9181 tttcacgacc tacatcttcc agtgttgccg cttcgtaccc cagcaaaccg aatcgacgtg
     9241 ccagcacttc acgctgtttg gcgttcagct cgaacagcca tttgacgatg ctctgcttca
     9301 tatcgtcatc ttgcgtggta tcttccggac cgttctcttt ttcatcggcc aggatgtcca
     9361 gcaacgcttt ttcggaatca ccacccagcg gggtgtctac cgaggtaatg cgctcgttaa
     9421 gacgaagcat acggctgacg tcatcaactg gcttatccag ttgctctgcg atctcttccg
     9481 cacttggttc atggtccagc ttatgggaca actcacgtgc ggttcgcagg taaacgttca
     9541 gctcctttac gatgtgaatc ggcaaacgaa tagtacgggt ttggttcata atcgcccgtt
     9601 caatcgtctg gcgaatccac caggttgcgt atgttgagaa gcggaaacca cgttccgggt
     9661 caaacttctc taccgcgcgg atcagcccca ggttgccctc ttcgataagg tccagcaacg
     9721 ccagaccacg attgccataa cggcgggcaa tttttaccac cagacgcaag ttactctcga
     9781 tcatccggcg gcgagaggcg acatctccac gcagtgcgcg acgcgcaaaa taaacttctt
     9841 cttcggccgt taacagtggt gaataaccaa tctcaccaag gtaaagctga gtcgcgtcca
     9901 acacacgctg tgtggctccc tgcgataaca gttcctcttc ggccaaatcg ttatcactgg
     9961 gttcctcttc tactaaggcc ttttcgtcaa aaacctcaac tccgttctca tcaaattccg
    10021 catcttcatt taaatcatga actttcagcg tattctgact cataaggtgg ctcctacccg
    10081 tgatcccttg acggaacatt caagcaaaag cctggttccg ccgatttatc gctgcggcaa
    10141 ataacgcagc gggtttacgg atttcccctt gtaacgaatt tcaaaatgca agcgtgttga
    10201 actggttccg gtgctaccca tggttgctat tttttgcccc gccttcactt cttgttgttc
    10261 ccggaccagc attgtgtcgt tatgggcgta ggcactcagg taatcatcat tatgtttgat
    10321 gataatcaga ttaccgtagc cgcgcagcgc gttaccggca taaacaacgc ggccatctgc
    10381 ggtcgcgata attgcctgtc ctttgctgcc tgcgatatca atccccttgt tgcccccctc
    10441 agaagcgcca aaggtttcga tcactttgcc ctcagtcggc cagcgccagg tggagatagg
    10501 cgtactggtt gatgtactgc tgacagttgg ctcggttgtg cttgctgttg gtaccgttac
    10561 aggcgctgtg accgtggtcg cagctggctt gttgttcggc aacattttgt tagcactctg
    10621 ttcacccgaa gattcagaat acgtaattgt cggttgcgac gcaacagcaa cggtggaatt
    10681 ttgtgcaggc ttgatcacaa ctccttgctc tgctgcgtcg gcctgggtaa tggcatttcc
    10741 gccagtgatt ggcgtaccgg aagcattacc cacctgcaag gtctgaccaa cgttcagcgc
    10801 gtatggtgcc tgaatattgt tgcgctgagc aaggtcacgg aaatcgttgc cagtaatcca
    10861 ggcgatatag aaaagtgtgt cgcctttttt cacggtatag gtactgccgc tataactgcc
    10921 tttcggaatg ttcccatact gacggttata gacgatgcgt ccgttttcca tctgtaccgg
    10981 ctgctgagct actggctgca ctggctggat ttgcggttgt tgagtagcct gaatttgtgg
    11041 ctgctgtacc ggctgaattt gcggttgctg ggctgtagac gtcgtcccca ttttcggcgg
    11101 cggcgtaatc aacataccag aattggtatt tgcaggcgca ttgccattaa cggagctgac
    11161 cggtgccggt ggatttgaag tgtcagaaca gcctgccagc catagcgaaa ccagtgacaa
    11221 agccgcaatg cggcgaacgg tgaattttgg gcttcccgcg ctcatttatc ccccaggaaa
    11281 aattggttaa taaccagtga cataattacc gtgcaaggca ccctactgaa cactggaaaa
    11341 gatgttcacg atacgctgac ctgcggcaaa ataaccagga aaaatccagg tatttcctca
    11401 cgttttaagc cagctcaccc ttcactaaag gtacaaagcg cacggcctcc acggtatcga
    11461 taataaattc gcctccccga cgacgcaccc gtttcaaata ctggtgctcc tcccctacgg
    11521 gtaagacgag aatcccgcct tcgtccagct gcgtcattag cgcagttgga atttccggcg
    11581 gtgccgccgt aacaatgata gcgtcaaacg gcgcgcgtgc ctgccaacct tgccatccat
    11641 cgccatgacg ggttgaaaca ttatgtaaat caagattttt caggcggcga cgcgcctgcc
    11701 actgcaaacc tttaatccgt tcgaccgagc aaacatgctg gacaagatgc gccaggattg
    11761 ccgtttgata tcccgaacca gtgccaattt ccagcacccg cgactgcggc gtcagctcga
    11821 gtaattcggt cattcgcgcc accatatacg gctgcgaaat tgtctgcccc tgacctatcg
    11881 gcaaggcgat attgtcccag gctttttgtt caaacgcttc atcaacgaat ttttcacgcg
    11941 gcacggcggc aagtgcattc agcacctgct catcctgaat accttgcgca cgtaattgat
    12001 ccagaagtgc ttgtacgcgt ctgcttacca ttgcgtgcca actcccacgc tgtttaacca
    12061 gtctgaaacc acatcttgcg cgctatgcgc agttaaatcc acatgcagcg gcgtgatgga
    12121 gacatagccc tcatctaccg cagcaaaatc ggtccccgga ccggcatcac atttaccgcc
    12181 cggcgggcca atccagtaca gcgtattgcc gcgcggatct tgctgcggga tcacctgatc
    12241 tgccggatgt cgtgtaccgc agcgcgtcac gcgaatacct ttgatttgat ccaagggtaa
    12301 atccggaacg ttaatattaa gaatacgccc ggtgcgcaac ggctctttac acagtgcgcg
    12361 caaaattgaa caggttaccg ccgcggcagt gtcgtaatgt ttatgcccgt caagcgagac
    12421 ggcaagcgcc ggaaaaccta aatgacggcc ttccatcgcg gcggctaccg taccggaata
    12481 aataacatca tcccccagat tcggcccggc gttaattccg gacacaacaa tgtccgggcg
    12541 cggacgcatc agagcattca cgccaagata gacgcaatcg gtcggggttc ccatttgcac
    12601 agcaatatca ccattttcaa aggtaaacgt gcgcagggag gattccagtg tcagagaatt
    12661 tgaagcgccg ctgcggttac gatcgggggc gaccacctga acgtcagcaa actcacgcaa
    12721 ggctttcgcc agcgtttgta taccgggtgc atgtaccccg tcatcattac tcagcaatat
    12781 gcgcataatc acctgttgtg ttgataagtt ccctgacaac gctggttgca aaactacccg
    12841 ccggaagcca gaaacggatc tctacggtga cgtcatccca ccaattccag cttaattgtt
    12901 gcggatacag cagcatcgct ctgcgcgcgg cttcaacttt ttcgcgcacc agtaaagctt
    12961 gtaattcagt ttctgcggcg acagctgctt gttcgaatgc cagcgcttca cgctgagttc
    13021 cccattcgcc actgcccggc aatgcggcgg ttatcatcag ctctttatcg ttaacgcgac
    13081 gctgtaattc cgccagttct tcggtggttg cgacaaacca gctaccacgt ccggctaatt
    13141 gtagcgcatc gccgtcaaca acttgattaa cgtctgcttt tttgaggcgc tcagcaacaa
    13201 tctgattaaa caacgcactg cgggctgccg acaaccaaaa actccgttta ttgcgatcgc
    13261 gcaccggagt attggtttgc gcccagcgct gcgccccctg caagttgcta ccgccaatcc
    13321 caaaacgttg ggcaccgaag tagttcggta caccttttac gcaaatatcg ttcagacgtt
    13381 gttcaacgtc atcgcgattg ctcacttcgc gcagaaccag ggtaaaggcg tttcctttca
    13441 gcgcccctaa acgcagcttg cgcttgtgcc gcgcatactc cagcacctgg cagccttcca
    13501 gttgaaaggc gctcagatcg ggcatttcct tgcccggcac gcgagcgcat aaccactgtt
    13561 ccgtaacagc atgtttgtct ttttgcccag cgaagctgac ttcacgggca tgaattttca
    13621 ggaatttcgc cagtgcatcc gccacaaaac gggtattgca gccgtttttg aggattctaa
    13681 ccagaatatg ctcaccttca ccatcaggct caaagcccaa atcttccacc accacaaagt
    13741 cttccggatt ggctttcagc agcccggtgc cttgcggttt accgtggagg taagtgagat
    13801 tatcaaactc aatcattttg ttgccttaat gagtagcgcc accgcttcac aggcaatccc
    13861 ttccccacgt ccggtaaatc ccagtttttc cgtagtagtg gctttcacgt taacatcatc
    13921 catatggcag ccgagatctt cggcaataaa cacgcgcatt tgtggaatgt gcggcaacat
    13981 cttcggtgcc tgagcgatga tagtgacatc gacgttgcca agggtataac ccttcgcctg
    14041 aatacgacgc caggcttcgc gtagcagctc gcggctatcg gcacctttaa atgccggatc
    14101 ggtatccggg aacagcttgc cgatatcccc cagcgccgcc gcgccaagca atgcatcggt
    14161 caacgcatgg agcgccacgt cgccatcaga atgcgccagc aatccttttt cgtaaggaat
    14221 gcgtacgcca ccaatgataa ttgggccttc accgccaaag gcatgtacgt caaaaccgtg
    14281 tccaattcgc attatgtatt ctcctgatgg atggttcggg tgaggtaaaa ctcggccagt
    14341 gccaaatctt ccgggcgcgt gactttaatg ttatccgcac ggccttcgac caactgagga
    14401 tggaatccgc aatattccag cgccgaggct tcgtcggtaa tagtcgcgcc ttcatttaga
    14461 gcgcgcgtca gacagtcatg taacagctca cgagggaaaa attgcggcgt cagcgcgtgc
    14521 cataagccgt tgcgatcaac ggtatgagca atggcatttt tgcccggttc ggcacgtttc
    14581 atagtatcgc gcactggtgc ggcgaggatc ccccccgtgc ggctggtttc gctcaacgcc
    14641 aacaatcgcg cgaggtcatc ctgatgcaaa caaggacgag cggcgtcatg caccaatacc
    14701 cactgcgcgt cgccagcggc tttcagacct gccagcacgg aatcggcacg ctcatcaccg
    14761 ccatctacaa cggtgatttg cggatgattc gccagaggaa gttgtgcaaa acggctatcg
    14821 ccaggactta tggcaatgac gactcgtttc acccggggat gcgccagcag cgcatgcacc
    14881 gagtgttcaa gaatggtttg attaccgatt gagagatatt gcttaggaca ttccgtttgc
    14941 attcgacggc caaatccggc cgccggaacc acggcgcaaa catccaaatg agtggttgcc
    15001 atgttaattc ccgggctgat ttatcgattg ttttgccccg cagactgtgc gcgcttcgac
    15061 gcgtcaggca ccagacgata aaaagtttcg cccggcctgg tcatgctgag ttcattacgc
    15121 gcacgctctt cgagcgcctc ctggccgcca ttgagatcgt caatttcggc aaaaagttga
    15181 tcgtttcgcg ctttaagttt cgcgtttgta gcttgctgtg ccgccacatc atcattgacg
    15241 cgggtatagt catgtatacc gttcttaccg aaccacagcg aatactgtag ccagaccaga
    15301 atagccagca acagcagcgt tagtttaccc atcctgcccc ctgaaaaacg gcatcatcat
    15361 cccatgcatc cgaagacgac tctacatcct ctgttgggga taccgcgaca acgcgggcaa
    15421 atgtaccaca tttgtccatt gttacgtata cccagggcgt gcagaacata atctcattat
    15481 tagttacggt ttgaattatg aacagaggag acgggaaagt acaaattagc ccagtagcca
    15541 cataaacagt gcgccaaaca taatgcctac tgtcaccagg gtgaaaacaa tactgtagcg
    15601 tagctttccg tccatcaatg aatgcagcgc aatccccacc actaccgcga cgggcatcag
    15661 cgccagaaag aaaggccagg tgtagataaa gaagaacagc gtgttagagc cataaatcaa
    15721 catcggcatc gccagcgcaa ataaccagga gataaaaccg accacggcac caggcagtga
    15781 ccatgtggtt tcttcatcct cagtaaggct gtcgttattt gttagtgtaa tgttatggct
    15841 attacgcata tttgatcctg ttactttgac gaaccgggca tggaaacccg gtggtgtctc
    15901 aggatctgat aatatcgttc tgtctcaaca gatctaataa ttgctgtacc aaatttgtta
    15961 ctaattgttc accattgaga tgaatttctg ccgattcagg cgcttcgtaa acggaatcta
    16021 ttcccgtaaa gttgcgcagt tcaccggcac gcgctttctt atataagcct tttggatcgc
    16081 gggcttcgca aatcgccagc ggcgtatcga caaacacttc gataaagcgc ccttctccta
    16141 cgcgttcgcg aaccatctgg cgttcggcgc ggtgtggcga gataaatgcg gtcagcacta
    16201 ccagtccggc ttcaaccatc aaattcgcca cttcaccgac gcgacggata ttctctttac
    16261 gatcggcatc gctaaaaccg agatcgctgc ataatccgtg gcgaacatta tcgccatcca
    16321 gcagatacgt actgacgccg agtttatgta acgcctcctc cagcgccccg gcgaccgttg
    16381 atttaccgga cccggagagg ccggtaaacc acagcactac accacgatga ccgtggtgta
    16441 gctcgcgttg ttgcacagtg accggatggc tatgccagac gacgttttcg tcatgcagcg
    16501 ccattattta tcccccagca aatcgcgcgc gccccagtgc ggaaagtggc gacgaaccag
    16561 agcattcaat tccagttcga atgcactgaa ttcagatggc gcagcagttg cctggctaac
    16621 tggctcgtgc accataccgg cacccacggt cacattgctc aggcgatcga taaaaatcag
    16681 cccacccgtc accggatttt gttgataacg atctaacacc agcggctcgt caaaagtgag
    16741 atccacgagg ccgatcccat tcagtggcag gttttcaact tcacgctggg taaggttatt
    16801 aatatcaacc tgatagcgaa tgccatcaac acgcgcgcgc gtcttcttac cggcaatttt
    16861 gatgtcgtaa ctctgccctg gagaaagcgg ctgttccgcc atccatacca catccaccga
    16921 cgcgctctgc accgccggta acgcttcgtc tgccgccagc agcagatcgc cacggctgat
    16981 gtcgatctca tccgtcagca ccagggtgat cgcttctccg gcaaaggctt cttcgcgatc
    17041 accatcaaaa gtcacgatcc gcgcgacgtt tgattccaca ccagagggca gcactttgac
    17101 acgttgcccg acttccacgc gaccggatgc cagcgttccg gcgtaaccac gaaaatcgag
    17161 attcgggcga ttaacgtact gcaccgggaa gcgcattggc tgagcatcca ccactcgctg
    17221 gatctccacg gtttccagca cttcgagcag tgtcggaccg ctgtaccacg gcatactttc
    17281 actttgcgat gccacgttgt cgccttccag tgcagagagc ggcacaaagc ggatatccag
    17341 attacccggc agctgcccgg caaaggtcaa ataatcttca cgaatacggg tgaacgtctc
    17401 ttcactgtaa tccaccagat ccattttgtt gatcgccacg accagatgtt tgctccccaa
    17461 cagtgtggag ataaaactgt gacgacgggt ttgatcgagc acgcctttac gggcatcgat
    17521 cagtaagatc gccagttcac atgtcgatgc gccagtcgcc atattgcggg tgtactgctc
    17581 gtgccctggg gtgtcggcga taataaattt acgcttctcg gtagagaaat agcggtaggc
    17641 cacgtcaatg gtgatgccct gttcgcgctc agcttgcagg ccgtccacca gcagagccag
    17701 atccagcttt tcgccctggg tgccgtgacg cttactgtcg ttatgcagcg atgagagctg
    17761 atcttcgtag atttggcggg tatcgtgcag cagacgacca atcagagtac ttttgccgtc
    17821 atcgacgcta ccacaggtca gaaaacgcag caggctttta tgttgttgcg caatcatcca
    17881 ggcttcgacg ccgccttcat tggcgatttg ttgtgcaagt gcggtgttca tcttaaaaat
    17941 acccctgacg ttttttcagc tccatagacc ccgcctggtc gcggtcaatc acgcggccct
    18001 gacgttcact ggtggtggaa accagcatct cttcgatgat ttccggcagt gtttgtgcat
    18061 ttgactccac cgcaccggtc agcggccagc agcccagcgt acggaaacgc accatccgtt
    18121 ttttaatcac ttcgcccggt tgcaggtcga tacggttgtc atcaatcatc atcaacatac
    18181 cgtcgcgttc cagaaccgga cgttccgcag cgagatatag cggaacaatg tcgatatttt
    18241 ccagccagat gtattgccag atatcctgct cggtccagtt agagagcggg aagacgcgga
    18301 tgctttcgcc tttgttaatt tgcccgttgt agttgtgcca cagctccggg cgctgatttt
    18361 tcggatccca gcgatggaag cggtcacgga aagagtaaat tcgctcttta gcgcgggatt
    18421 tctcttcgtc acggcgcgca ccaccgaagg cggcatcaaa accgtatttg ttcagcgcct
    18481 gtttcaggcc ttcagttttc ataatatcgg tatgtttcgc gctgccgtgc acgaatggat
    18541 taatccccat cgccacgcct tccgggtttt tatgcaccag cagttcgcag ccgtaggctt
    18601 tagcagtacg atcgcggaac tcatacatct cgcggaattt ccagccggta tcgacatgca
    18661 gcaacgggaa aggcagcgta cctggataaa acgccttgcg cgccagatgc agcatgacgc
    18721 tggaatcttt accgatagag tagagcatca ccggatttga gaattctgcc gccacctcgc
    18781 gaataatgtg gatgctttcc gcctccagtt gccgcaggtg agtaagtcgt atttgatcca
    18841 taaccgttcc tttgcaatac cgctattttc ttgccatcag atgtttcgac tatagggagc
    18901 gtaagagaac gaatgaaatt accaattaga atgagtagtt ccttaacgga ataacgattt
    18961 ggcaaagcta atatcaaaaa gtgcttaagg caccggattt cgggcgttta ggaagatttg
    19021 aaattgtttt agcgcagcgg cagtttcata ctatggcggt aaaaaaattt gcatggtatt
    19081 taaggactca ctatgttttc cgcattgcgc caccgtaccg ctgccctggc gctcggcgta
    19141 tgctttattc tccccgtaca cgcctcgtca cctaaacctg gcgattttgc taatactcag
    19201 gcacgacata ttgctacttt ctttccggga cgcatgaccg gaactcctgc agaaatgtta
    19261 tctgccgatt atattcgcca acagtttcag caaatgggtt atcgcagtga tattcggaca
    19321 tttaatagtc ggtatattta taccgcccgc gataatcgca agagctggca taacgtgacg
    19381 ggaagtacgg tgattgccgc tcatgaaggc aaagcgccgc agcagatcat cattatggcg
    19441 catctggata cttacgcccc gctgagcgat gctgacgccg atgccaatct cggcgggctt
    19501 acgttacaag gaatggatga taacgccgca ggtttaggtg tcatgctgga attggcagaa
    19561 cgcctgaaaa atacgcctac cgagtatggt attcgatttg tggcgaccag cggcgaagag
    19621 gaagggaaat taggcgcaga gaatttactc aagcggatga gtgacaccga aaagaaaaat
    19681 acgctgctgg tgattaatct cgataactta attgttggcg ataaattgta tttcaacagc
    19741 ggtgtaaaaa cccctgaagc agtaaggaaa ttaacgcgcg acagggcgct ggcaattgcg
    19801 cgtagtcatg gaattgccgc aacgaccaat ccgggtttga ataaaaatta tccgaaaggc
    19861 actgggtgtt gtaatgacgc agaaatattc gacaaagcgg gcattgctgt actttcggtg
    19921 gaagcgacta actggaatct tgggaataag gatggttatc agcaacgcgc aaaaacacct
    19981 gccttcccgg cgggaaatag ctggcatgac gtaagactgg ataatcaaca acatattgat
    20041 aaggcacttc ctggaagaat agaacgtcgc tgccgtgatg ttatgcggat aatgctaccg
    20101 ctggtgaagg agctggcgaa ggcgtcttga tgggtttgaa aatgggagct gggagttcta
    20161 ccgcaggggc ggggaattct aagtgatatc catcatcgca tccagtgcgc ccggtttatc
    20221 cccgctgacg cggggaacac aaaaccaaac ttctccataa attccatagc cgcggtttat
    20281 ccccgctggc gcggggaaca cgagtctatc agcgacacta ccggcaatag cgacggttta
    20341 tccccgctgg cgcggggaac acgagagtgc tgacaggtgt ctcgattacc tgatcggttt
    20401 atccccgctg gcgcggggaa cacggtagta cgcgcctccg gacgttttta tgtcgcggtt
    20461 tatccccgct ggcgcgggga acactcttcg cgggtaatca atgatgattc agtttccggt
    20521 ttatccccgc tggcgcgggg aacacgaacg caatatcacg gcgttctgcg gcctcggcgg
    20581 tttatccccg ctggcgcggg gaacaccaaa atattacgag cttcgtcagg ccatggaccg
    20641 gtttatcccc gctggcgcgg ggaacacgtc gggccgggta agtgaacctg tcggttttcc
    20701 ggtttatccc cgct
//
LOCUS       DAZKHF020000064        20538 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2201S31241
            SAMN43267800-rid25106413.denovo.064, whole genome shotgun sequence.
ACCESSION   DAZKHF020000064 DAZKHF020000000
VERSION     DAZKHF020000064.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267800
            Sequence Read Archive: SRR30307263
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 20538)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20538)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 20538)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350581.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350581.2
            Genome Coverage       :: 151x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:21:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,652
            CDSs (total)                      :: 5,557
            Genes (coding)                    :: 5,320
            CDSs (with protein)               :: 5,320
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 237
            CDSs (without protein)            :: 237
            Pseudo Genes (ambiguous residues) :: 0 of 237
            Pseudo Genes (frameshifted)       :: 102 of 237
            Pseudo Genes (incomplete)         :: 130 of 237
            Pseudo Genes (internal stop)      :: 52 of 237
            Pseudo Genes (multiple problems)  :: 41 of 237
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20538
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267800-rid25106413.denovo.064"
                     /serotype="O 26:H11"
                     /isolate="2201S31241"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-01"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            complement(1..1260)
                     /locus_tag="ACHOSC_RS09540"
                     /old_locus_tag="pgaptmp_001908"
                     /pseudo
     CDS             complement(1..1260)
                     /locus_tag="ACHOSC_RS09540"
                     /old_locus_tag="pgaptmp_001908"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416950.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0004029 - aldehyde dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_function: GO:0008774 - acetaldehyde dehydrogenase
                     (acetylating) activity [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
     gene            complement(1272..1559)
                     /gene="eutN"
                     /locus_tag="ACHOSC_RS09545"
                     /old_locus_tag="pgaptmp_001909"
     CDS             complement(1272..1559)
                     /gene="eutN"
                     /locus_tag="ACHOSC_RS09545"
                     /old_locus_tag="pgaptmp_001909"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization microcompartment
                     protein EutN"
                     /protein_id="HGU4547857.1"
                     /translation="MKLAVVTGQIVCTVRHHGLAHDKLLMVEMIDPQGNPDGQCAVAI
                     DNIGAGTGEWVLLVSGSSARQAHKSETSPVDLCVIGIVDEVVSGGQVIFHK"
     gene            complement(1666..1959)
                     /gene="eutM"
                     /locus_tag="ACHOSC_RS09550"
                     /old_locus_tag="pgaptmp_001910"
     CDS             complement(1666..1959)
                     /gene="eutM"
                     /locus_tag="ACHOSC_RS09550"
                     /old_locus_tag="pgaptmp_001910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416952.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization microcompartment
                     protein EutM"
                     /protein_id="HGU4547858.1"
                     /translation="MEALGMIETRGLVALIEASDAMVKAARVKLVGVKQIGGGLCTAM
                     VRGDVAACKAATDAGAAAAQRIGELVSVHVIPRPHGDLEEVFPIGLKGDSSNL"
     gene            complement(1998..3014)
                     /gene="pta"
                     /locus_tag="ACHOSC_RS09555"
                     /old_locus_tag="pgaptmp_001911"
     CDS             complement(1998..3014)
                     /gene="pta"
                     /locus_tag="ACHOSC_RS09555"
                     /old_locus_tag="pgaptmp_001911"
                     /EC_number="2.3.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008959 - phosphate acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006086 - acetyl-CoA biosynthetic process
                     from pyruvate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acetyltransferase"
                     /protein_id="HGU4547859.1"
                     /translation="MIIERCRELALRAPARVVFPDALDQRVLKAAQYLHQQGLATPIL
                     VANPFELRQFALSHGVAMDGLQVIDPHGNLAMREEFAHRWLARAGEKTPPDALEKLTD
                     PLMFAAAMVSAGKADVCIAGNLSSTANVLRAGLRIIGLQPGCKTLSSIFLMLPQYSGP
                     ALGFADCSVVPQPTAAQLADIALASAETWRAITGEEPRVAMLSFSSNGSARHPCVANV
                     QQATEIVRERAPKLVVDGELQFDAAFVPEVAAQKAPASPLQGKANVMVFPSLEAGNIG
                     YKIAQRLGGYRAVGPLIQGLAAPMHDLSRGCSVQEIIELALVAAVPRQTEVNRESSLQ
                     TLVE"
     gene            complement(3011..3814)
                     /gene="eutT"
                     /locus_tag="ACHOSC_RS09560"
                     /old_locus_tag="pgaptmp_001912"
     CDS             complement(3011..3814)
                     /gene="eutT"
                     /locus_tag="ACHOSC_RS09560"
                     /old_locus_tag="pgaptmp_001912"
                     /EC_number="2.5.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008817 - corrinoid adenosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006580 - ethanolamine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization cob(I)yrinic acid
                     a,c-diamide adenosyltransferase EutT"
                     /protein_id="HGU4547860.1"
                     /translation="MKDFITEAWLRANHTLSEGAEIHLPADSRLTPSARELLESRHLR
                     IKFIDEQGRLFVDDEQQQPQPVHGLTSSDEHPQACCELCRQPVAKKPDTLTHLSAEKM
                     VAKSDPRLGFRAVLDSTIALAVWLQIELAEPWQPWLADIRSRLGNIMRADALGEPLGC
                     QAIVGLSDEDLHRLSHQPLRYLDHDHLVPEASHGRDAALLNLLRTKVRETETVAAQVF
                     ITRSFEVLRPDILQALNRLSSTVYVMMILSVTKQPLTVKQIQQRLGETQ"
     gene            complement(3811..4512)
                     /gene="eutQ"
                     /locus_tag="ACHOSC_RS09565"
                     /old_locus_tag="pgaptmp_001913"
     CDS             complement(3811..4512)
                     /gene="eutQ"
                     /locus_tag="ACHOSC_RS09565"
                     /old_locus_tag="pgaptmp_001913"
                     /EC_number="2.7.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization acetate kinase EutQ"
                     /protein_id="HGU4547861.1"
                     /translation="MKKLITANDIREAHARGEQAMSVVLRASIITPEAREVADLLGFT
                     ITECDESIPVTASVPASVPADKTESQRIRETIIAQLPEGQFTESLVAQLMEKVMKEKQ
                     SLEQGAMQPSFKSVTGKGGIKVIDGSSVKFGRFDGAEPHCVGLTDLVTGDDGSSMAAG
                     FMQWENAFFPWTLNYDEIDMVLEGELHVRHEGETMIAKAGDVMFIPKGSSIEFGTTSS
                     VKFLYVAWPANWQSL"
     gene            complement(4487..4966)
                     /gene="eutP"
                     /locus_tag="ACHOSC_RS09570"
                     /old_locus_tag="pgaptmp_001914"
     CDS             complement(4487..4966)
                     /gene="eutP"
                     /locus_tag="ACHOSC_RS09570"
                     /old_locus_tag="pgaptmp_001914"
                     /EC_number="2.7.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization acetate kinase EutP"
                     /protein_id="HGU4547862.1"
                     /translation="MKRIAFVGTVGAGKTTLFNALQGNYTLARKTQAVEFNDKGDIDT
                     PGEYFSHPRWYHALITTLQDVDMLIYVHGANDPESRLPAGLLDIGVSKRQIAVISKTD
                     MPDADVAATRKLLLETGFEEPIFELNSHDPQSVQQLVDYLASLTKQEEAGEKTHHSE"
     gene            complement(4979..5314)
                     /gene="eutS"
                     /locus_tag="ACHOSC_RS09575"
                     /old_locus_tag="pgaptmp_001915"
     CDS             complement(4979..5314)
                     /gene="eutS"
                     /locus_tag="ACHOSC_RS09575"
                     /old_locus_tag="pgaptmp_001915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311351.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization microcompartment
                     protein EutS"
                     /protein_id="HGU4547863.1"
                     /translation="MDKERIIQEFVPGKQVTLAHLIAHPGEELAKKIGVPDAGAIGIM
                     TLTPGETAMIAGDLALKAADVHIGFLDRFSGALVIYGSVGAVEEALSQTVSGLGRLLN
                     YTLCEMTKS"
     gene            complement(5607..7886)
                     /gene="maeB"
                     /locus_tag="ACHOSC_RS09580"
                     /old_locus_tag="pgaptmp_001916"
     CDS             complement(5607..7886)
                     /gene="maeB"
                     /locus_tag="ACHOSC_RS09580"
                     /old_locus_tag="pgaptmp_001916"
                     /EC_number="1.1.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent oxaloacetate-decarboxylating
                     malate dehydrogenase"
                     /protein_id="HGU4547864.1"
                     /translation="MDDQLKQSALDFHEFPVPGKIQVSPTKPLATQRDLALAYSPGVA
                     APCLEIEKDPLKAYKYTARGNLVAVISNGTAVLGLGNIGALAGKPVMEGKGVLFKKFA
                     GIDVFDIEVDELDPDKFIEVVAALEPTFGGINLEDIKAPECFYIEQKLRERMNIPVFH
                     DDQHGTAIISTAAILNGLRVVEKNISDVRMVVSGAGAAAIACMNLLVALGLQKHNIVV
                     CDSKGVIYQGREPNMAETKAAYAVVDDGKRTLDDVIEGADIFLGCSGPKVLTQEMVKK
                     MARAPMILALANPEPEILPPLAKEVRPDAIICTGRSDYPNQVNNVLCFPFIFRGALDV
                     GATAINEEMKLAAVRAIAELAHAEQSEVVASAYGDQDLSFGPEYIIPKPFDPRLIVKI
                     APAVAKAAMESGVATRPIADFDVYIDKLTEFVYKTNLFMKPIFSQARKAPKRVVLSEG
                     EEARVLHATQELVTLGLAKPILIGRPNVIEMRIQKLGLQIKAGVDFEIVNNESDPRFK
                     EYWTEYFQIMKRRGVTQEQAQRALISNPTVIGAIMVQRGEADAMICGTVGDYHEHFSV
                     VKNVFGYRDGVHTAGAMNALLLPSGNTFIADTYVNDEPDAEELAEITLMAAETVRRFG
                     IEPRVALLSHSNFGSSDCPSSSKMRQALELVRDRAPDLMIDGEMHGDAALVEAIRNDR
                     MPDSPLKGSANILVMPNMEAARISYNLLRVSSSEGVTVGPVLMGVAKPVHVLTPIASV
                     RRIVNMVALAVVEAQTQPL"
     gene            8175..9125
                     /gene="tal"
                     /locus_tag="ACHOSC_RS09585"
                     /old_locus_tag="pgaptmp_001917"
     CDS             8175..9125
                     /gene="tal"
                     /locus_tag="ACHOSC_RS09585"
                     /old_locus_tag="pgaptmp_001917"
                     /EC_number="2.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004801 - transaldolase activity [Evidence
                     IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transaldolase"
                     /protein_id="HGU4547865.1"
                     /translation="MNELDGIKQFTTVVADSGDIESIRHYHPQDATTNPSLLLKAAGL
                     SQYEHLIDDAIAWGKKNGKTQEQQVVAACDKLAVNFGAEILKIVPGRVSTEVDARLSF
                     DKEKSIEKARHLVDLYQQQGVEKSRILIKLASTWEGIRAAEELEKEGINCNLTLLFSF
                     AQARACAEAGVFLISPFVGRIYDWYQARKPMDPYVVEEDPGVKSVRNIYDYYKQHHYE
                     TIVMGASFRRTEQILALTGCDRLTIAPNLLKELQEKVSPVVRKLIPPSQTFPRPAPMS
                     EAEFRWEHNQDAMAVEKLSEGIRLFAVDQRKLEDLLAAKL"
     gene            9145..11148
                     /gene="tkt"
                     /locus_tag="ACHOSC_RS09590"
                     /old_locus_tag="pgaptmp_001918"
     CDS             9145..11148
                     /gene="tkt"
                     /locus_tag="ACHOSC_RS09590"
                     /old_locus_tag="pgaptmp_001918"
                     /EC_number="2.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004802 - transketolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HGU4547866.1"
                     /translation="MSRKDLANAIRALSMDAVQKANSGHPGAPMGMADIAEVLWNDFL
                     KHNPTDPTWYDRDRFILSNGHASMLLYSLLHLTGYDLPLEELKNFRQLHSKTPGHPEI
                     GYTPGVETTTGPLGQGLANAVGLAIAERTLAAQFNQPDHEIVDHFTYVFMGDGCLMEG
                     ISHEVCSLAGTLGLGKLIGFYDHNGISIDGETEGWFTDDTAKRFEAYHWHVIHEIDGH
                     DPQAVKEAILEAQSMKDKPSLIICRTVIGFGSPNKAGKEEAHGAPLGEEEVALARQKL
                     GWHHPPFEIPKKIYHAWDAREKGEKAQQSWNEKFAAYKKAHPQLAEEFTRRMSGGLPK
                     DWEKTTQKYINELQANPAKIATRKASQNTLNAYGPMLPELLGGSADLAPSNLTIWKGS
                     VSLKEDPAGNYIHYGVREFGMTAIANGIAHHGGFVPYTATFLMFVEYARNAARMAALM
                     KARQIMVYTHDSIGLGEDGPTHQAVEQLASLRLTPNFSTWRPCDQVEAAVGWKLAVER
                     HNGPTALILSRQNLAQVERTPDQVKEIARGGYVLKDSGGKPDIILIATGSEMEITLQA
                     AEKLAGEGRNVRVVSLPSTDIFDAQDEEYRESVLPSNVAARVAVEAGIADYWYKYVGL
                     KGVIVGMTGYGESAPADKLFPFFGFTAENIVAKAHKVLGVKGA"
     gene            complement(11244..12287)
                     /gene="ypfG"
                     /locus_tag="ACHOSC_RS09595"
                     /old_locus_tag="pgaptmp_001919"
     CDS             complement(11244..12287)
                     /gene="ypfG"
                     /locus_tag="ACHOSC_RS09595"
                     /old_locus_tag="pgaptmp_001919"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708305.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1176 domain-containing protein"
                     /protein_id="HGU4547867.1"
                     /translation="MRYRIFLLFFFALLPTSLVWAAPAQRAFSDWQVTCNNQNFCVAR
                     NTGDHNGLVMTLSRSAGAHTDAVLRIERGGLKSPDASEGEIAPRLLLDGEPLALSGDK
                     WRISPWLLVTDDTATITAFLQVIQEGKAITLRDGNQTISLSGLKAALLFIDAQQKRVG
                     SETAWIKKGDEPPLSVPPAPALKEVAVVNPTPTPLSLEERNDLLDYGNWRMNGLRCSL
                     DPLRREVNVTALTDDKALMMISCEAGAYNTIDLAWIVSRKKPLASRPVRLRLPFNNGQ
                     ETNELELMNATFDEKSRELVTLAKGRGLSDCGIQARWRFDGQRFRLVRYAAEPTCDNW
                     HGPDAWPTLWITR"
     gene            complement(12413..12988)
                     /gene="nudK"
                     /locus_tag="ACHOSC_RS09600"
                     /old_locus_tag="pgaptmp_001920"
     CDS             complement(12413..12988)
                     /gene="nudK"
                     /locus_tag="ACHOSC_RS09600"
                     /old_locus_tag="pgaptmp_001920"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016818 - hydrolase activity, acting on
                     acid anhydrides, in phosphorus-containing anhydrides
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-mannose pyrophosphatase NudK"
                     /protein_id="HGU4547868.1"
                     /translation="MTQQITLIKDKILSDNYFTLHNITYDLTRKDGEVIRHKREVYDR
                     GNGATILLYNAKKKSVVLIRQFRVATWVNGNESGQLIETCAGLLDNDEPEVCIRKEAI
                     EETGYEVGEVRKLFELYMSPGGVTELIHFFIAEYSDNQRANAGGGVEDEDIEVLELPF
                     SQALEMIKTGEIRDGKTVLLLNYLQTSHLMD"
     gene            complement(13056..15035)
                     /gene="aegA"
                     /locus_tag="ACHOSC_RS09605"
                     /old_locus_tag="pgaptmp_001921"
     CDS             complement(13056..15035)
                     /gene="aegA"
                     /locus_tag="ACHOSC_RS09605"
                     /old_locus_tag="pgaptmp_001921"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate-dependent uric acid utilization protein
                     AegA"
                     /protein_id="HGU4547869.1"
                     /translation="MNRFIMANSQQCLGCHACEIACVMAHNDEQHVLSQHHFHPRITV
                     IKHQQQRSAVTCHHCEDAPCARSCPNGAISHVDDSIQVNQQKCIGCKSCVVACPFGTM
                     QIVLTPVAAGKVKATAHKCDLCAGRENGPACVENCPADALQLVTDVALSGMAKPRRLR
                     TARQEHQPWHASTAAQEMPVMSKVEQMQATPARGEPDKLAIEARKTGFDEIYLPFRAD
                     QAQREASRCLKCGEHSVCEWTCPLHNHIPQWIELVKAGNIDAAVELSHQTNTLPEITG
                     RVCPQDRLCEGACTIRDEHGAVTIGNIERYISDQALAKGWRPDLSHVTKVDKRVAIIG
                     AGPAGLACADVLTRNGVAVTVYDRHPEIGGLLTFGIPSFKLDKSLLARRREIFSAMGI
                     HFELNCEVGKDVSLDSLLEQYDAVFVGVGTYRSMNAGLPNEDAPGVYDALPFLIANTK
                     QVMGLEELPEEPFINTAGLNVVVLGGGDTAMDCVRTALRHGASNVTCAYRRDEANMPG
                     SKKEVRNAREEGANFEFNVQPVALELNEQGHVCGIRFLRTRLGEPDAQGRRRPVPIEG
                     SEFVMPADAVIMAFGFNPHGMPWLESHGVTVDKWGRIIADVESQYRYQTTNPKIFAGG
                     DAVRGADLVVTAMAEGRHAAQGIIDWLGVKSVKSH"
     gene            15241..16941
                     /gene="narQ"
                     /locus_tag="ACHOSC_RS09610"
                     /old_locus_tag="pgaptmp_001922"
     CDS             15241..16941
                     /gene="narQ"
                     /locus_tag="ACHOSC_RS09610"
                     /old_locus_tag="pgaptmp_001922"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate/nitrite two-component system sensor
                     histidine kinase NarQ"
                     /protein_id="HGU4547870.1"
                     /translation="MIVKRPVSASLARAFFYIVLLSILSTGIALLTLASSLRDAEAIN
                     IAGSLRMQSYRLGYDLQSGSPQLNAHRQLFQQALHSPVLTNLNVWYVPEAVKTRYAHL
                     NANWLEMNNRLSKGDLPWYQANINNYVNQIDLFVLALQHYAERKMLLVVAISLAGGIG
                     IFTLVFFTLRRIRHQVVAPLNQLVTASQRIEHGQFDSPPLDTSLPNELGLLAKTFNQM
                     SSELHKLYLSLEASVEEKTRDLHEAKRRLEVLYQCSQALNTSQIDVHCFRHILQIVRD
                     NEAAEYLELNVGENWRISEGQPNPELPMQILPVTMQETVYGELHWQNSHVSSSEPLLN
                     SVSSMLGRGLYFNQAQKHFQQLLLMEERATIARELHDSLAQVLSYLRIQLTLLKRSIP
                     EDNATAQSIMADFSQALNDAYRQLRELLTTFRLTLQQADLPSALREMLDTLQNQTSAK
                     LTLDCRLPTLALDAQMQVHLLQIIREAVLNAMKHANASEIAVSCVTAPDGNHTVYIRD
                     NGIGIGEPKEPEGHYGLNIMRERAERLGGTLTFSQPSGGGTLVSISFRSAEGEESQLM
                     "
     gene            17105..20218
                     /gene="acrD"
                     /locus_tag="ACHOSC_RS09615"
                     /old_locus_tag="pgaptmp_001923"
     CDS             17105..20218
                     /gene="acrD"
                     /locus_tag="ACHOSC_RS09615"
                     /old_locus_tag="pgaptmp_001923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0042908 - xenobiotic transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter permease AcrD"
                     /protein_id="HGU4547871.1"
                     /translation="MANFFIDRPIFAWVLAILLCLTGTLAIFSLPVEQYPDLAPPNVR
                     VTANYPGASAQTLENTVTQVIEQNMTGLDNLMYMSSQSSGTGQASVTLSFKAGTDPDE
                     AVQQVQNQLQSAMRKLPQAVQNQGVTVRKTGDTNILTIAFVSTDGSMDKQDIADYVAS
                     NIQDPLSRVNGVGDIDAYGSQYSMRIWLDPAKLNSFQMTAKDVTDAIESQNAQIAVGQ
                     LGGTPSVDKQALNATINAQSLLQTPEQFRDITLRVNQDGSEVRLGDVATVEMGAEKYD
                     YLSRFNGKPASGLGVKLASGANEMATAELVLNRLDELAQYFPHGLEYKVAYETTSFVK
                     ASIEDVVKTLLEAIALVFLVMYLFLQNFRATLIPTIAVPVVLMGTFSVLYAFGYSVNT
                     LTMFAMVLAIGLLVDDAIVVVENVERIMSEEGLTPREATRKSMGQIQGALVGIAMVLS
                     AVFVPMAFFGGTTGAIYRQFSITIVAAMVLSVLVAMILTPALCATLLKPLKKGEHHGQ
                     KGFFAWFNQMFNRNAERYEKGVAKILHRSLRWIVIYVLLLGGMVFLFLRLPTSFLPQE
                     DRGMFTTSVQLPSGSTQQQTLKVVEQIEKYYFTHEKDNIMSVFATVGSGPGGNGQNVA
                     RMFIRLKDWSERDSKTGTSFAIIERATKAFNQIKEARVIASSPPAISGLGSSAGFDME
                     LQDHAGAGHDALMAARNQLLALAAENPELTRVRHNGLDDSPQLQIDIDQRKAQALGVA
                     IDDINDTLQTAWGSSYVNDFMDRGRVKKVYVQAAAPYRMLPDDINLWYVRNKDGGMVP
                     FSAFATSRWETGSPRLERYNGYSAVEIVGEAAPGVSTGTAMDIMESLVKQLPNGFGLE
                     WTAMSYQERLSGAQAPALYAISLLVVFLCLAALYESWSVPFSVMLVVPLGVIGALLAT
                     WMRGLENDVYFQVGLLTVIGLSAKNAILIVEFANEMNQKGHDLFEATLHACRQRLRPI
                     LMTSLAFIFGVLPMATSTGAGSGGQHAVGTGVMGGMISATILAIYFVPLFFVLVRRRF
                     PLKPRPE"
     gene            complement(20317..20376)
                     /gene="ypfM"
                     /locus_tag="ACHOSC_RS09620"
                     /old_locus_tag="pgaptmp_001924"
     CDS             complement(20317..20376)
                     /gene="ypfM"
                     /locus_tag="ACHOSC_RS09620"
                     /old_locus_tag="pgaptmp_001924"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YpfM"
                     /protein_id="HGU4547872.1"
                     /translation="MIERELGNWKDFIEVMLRK"
BASE COUNT         4690 a         5602 c         5358 g         4888 t
ORIGIN      
        1 gtccgttctt aacgaaaatg ctggtatcaa tggcgttcgc catctggttc atgttttcga
       61 tattgcgcga gtgcattgcc gccgtgtggt ggcaaccgcc ctccagtttc accgccagcg
      121 caatggcatc cgccacgttg gcgacgcgca cgacgggcag caccggcatc atcagttcgg
      181 tcacggcaaa cggatgttct gcagtggttt ccacaaacag caaacgcgtt tcttgcggca
      241 cgttcaggcc gattgccgcc gcgattttcg ccgcatcgcg accaacccag tcacggctga
      301 cggtgccttt tccgcgctcg tcgatatttt tcagcaacac cggttgcagc tgctgcgcct
      361 gttctgcggt cagtttcacc gcgtgctggc cttccatcag acgcatcagt tcatcggcta
      421 cgctatcaac aacaatcagt accttttcgt cggcacaaat gatgttgtta tcgaaagaag
      481 cgcctttgac gatggactga gcggcacggg cgaggtcggc ggtttcatcc accactaccg
      541 gcgggttgcc agcgcctgcg gcaatcagac gtttattggt gtgtttacgc gccgcttcta
      601 ctaccgcttc gccgccggtt accaccagca ggccgatacc cggaaacttg aacaagcgtt
      661 gcgcggtttc gatatccgga tttgccacag taaccagtaa gttttccggc ccacctgcgg
      721 caacaatcgc ctggttgagc agcgtaatcg cccgctggga gacttttttc gccgccggat
      781 gcggggcaaa aatgacgctg ttgcccgcgc aatcaggctg atggcgttgt taattacggt
      841 tgccgccggg ttagtggaag gcgtcaccga agccaccacg ccccagggtg cgttttcaat
      901 tagggtcagg ccgttgtcgc cagtcagcac ttgcggagag aggcactcaa cgcctggtgt
      961 gccgcgcgcc tgagcgacgt tttttgcaaa tttatcttca acgcgcccca tgccggtttc
     1021 actgacggca agttccgcta aatctctggc gtgtttttcg cctgcttcac gaatggcagc
     1081 aatggctaac tggcgcattg ccacgctttt taacccttgc tgggcgactt tggctgccgc
     1141 aacggcgtca tccagggacg cgaaaacgcc catctcatga acggcggcgg acggcgtgtc
     1201 actgctttgc attttcagca gtaccgcttt caccacctgt tcaatatcct gttgattcat
     1261 gatgttctgc cttatttgtg gaaaattacc tgaccgccag acaccacctc atcgacaatg
     1321 ccaatcacgc acagatcgac cggtgacgtt tcgcttttat gcgcctggcg ggcggaactg
     1381 ccactcacca gtaacaccca ctccccggtt cccgcgccaa tattgtcgat ggcgacggcg
     1441 cattgcccgt caggattacc ttgtggatca atcatttcca ccatcagcaa tttgtcatgc
     1501 gccagtccgt gatggcgtac ggtacaaaca atttgtccag tgacgactgc cagtttcata
     1561 cccgcctccg tggcgtattt caggtaaaag ctccccctac cctccgcaga aggtaaaatg
     1621 aaaaaggaga gagcgtgacg cccgaatcga cgtcacacag ggtgattaca ggttgctgct
     1681 atcgcctttc aggccgatcg ggaagacttc ttccaggtcg ccgtgcggac gcgggataac
     1741 gtgtacagat accagctcgc cgatacgctg tgccgctgcg gcacctgcat cggtcgcggc
     1801 tttacaggct gcaacatcac cacgaaccat ggcagtacac aggccgccgc caatctgctt
     1861 aacaccaacc agcttgacgc gcgcagcttt caccatcgcg tcagaagcct caatcagtgc
     1921 aaccaggccc cgggtttcga tcattcctaa tgcttccatt gtgctttcct ctttatcagg
     1981 gtccagaacg ggaccgttca ttcaaccagt gtttgtaaac tgctttcgcg gttcacttct
     2041 gtctgacgcg gcacagctgc caccagcgcc agctcgataa tttcctgcac gctacaacca
     2101 cgagagagat cgtgcatcgg cgcggcaagt ccttgtatca gtggcccgac ggcacgatat
     2161 ccgccgagtc gttgtgcgat tttgtaacca atatttccgg cttccagcga cggaaaaacc
     2221 atcacattgg ccttgccctg tagcgggctg gcaggcgctt tttgcgccgc cacttccggc
     2281 acgaaggcgg cgtcaaactg taactcgcca tccaccacca gctttggtgc gcgctcacgg
     2341 acgatttctg tcgcctgctg gacgttagca acacaggggt gacgggcgct accattgctg
     2401 gaaaacgaca gcatcgccac gcgcggctct tctccggtga tggcgcgcca ggtttcggca
     2461 ctggcaagcg cgatatccgc cagctgcgcc gccgtcggct gtggcaccac gctgcaatcg
     2521 gcaaagccca acgccgggcc gctgtactgt ggcaacatca ggaaaatgga agagagcgtt
     2581 ttacagcctg gctgcaagcc gataatgcgt aatccggcac gcagcacatt tgccgtggaa
     2641 gagaggttgc ccgcgataca gacatccgct ttaccggcgc tgaccattgc tgcggcgaac
     2701 atcagagggt cggtaagttt ttccagcgca tccggcggcg ttttttcgcc cgcgcgggcc
     2761 agccagcgat gagcaaattc ttcccgcatt gcgaggttgc catgcggatc aatcacctgt
     2821 agcccgtcca tcgccacgcc gtgactgagc gcaaactgac gaagttcaaa cggattggcg
     2881 accagaatgg gcgttgccag accttgttga tgtaaatatt gcgcggcttt cagcacacgt
     2941 tggtctaacg catccggaaa aaccactctg gcgggcgctc gcaacgccag ttcacgacaa
     3001 cgttcaataa tcattgcgtt tcccccagtc gttgttggat ctgcttcacc gttagcggct
     3061 gcttcgttac gctcaggatc atcatcacgt agaccgtgct ggaaaggcgg ttcagcgcct
     3121 gcaaaatgtc cgggcgtaat acttcaaagc tgcgggtgat aaacacctgc gccgctacgg
     3181 tttcggtttc ccgcactttg gtacgtagca gattgagcaa cgccgcatcg cgcccgtggc
     3241 tggcttccgg caccagatga tcgtggtcga gatagcgcag cggctggtga gaaagtcgat
     3301 gcagatcttc gtcagaaagc ccgacaatcg cctggcagcc cagcggttca cccagcgcat
     3361 cggcgcgcat aatgttgcca agacgcgaac ggatatccgc cagccacggc tgccacggtt
     3421 ccgccagttc aatttgcagc cacactgcca gcgcaatggt gctgtcgaga accgcgcgaa
     3481 aacccagacg cggatcgctt ttggcgacca ttttttccgc cgacaggtga gtcagcgtat
     3541 ccggcttttt tgccaccggc tggcggcaca gttcgcagca cgcctgcgga tgttcatcgc
     3601 tactggtcag cccatgaacg ggctgcggct gctgctgttc atcgtcaaca aacaggcggc
     3661 cctgctcgtc aataaacttg atgcgcagat ggcggctttc cagtaactcc cgggcagagg
     3721 gcgtcaggcg actgtccgca gggagatgga tctctgcgcc ttcgctgagc gtatggttcg
     3781 ctcttagcca tgcttcggtg atgaaatctt tcatagggat tgccagttag ccggccaggc
     3841 gacatacagg aatttcacgc tggatgtcgt accaaattcg atactggagc ctttcgggat
     3901 aaacatcacg tcacccgctt tggcgatcat ggtttcgcct tcgtggcgaa catgcagctc
     3961 cccttccagc accatgtcga tttcgtcgta gttcagggtc cacgggaaga atgcgttctc
     4021 ccactgcata aaaccagcgg ccatgctgct gccgtcgtct ccggtcacaa gatcagtcaa
     4081 gcccacgcaa tgcggctctg cgccgtcgaa acgaccaaac ttgacgctgc tgccatcaat
     4141 cactttgatg ccgcctttgc cggtcaccga tttgaagctc ggctgcattg cgccctgctc
     4201 cagcgactgt ttttccttca tcactttttc catcagctgt gcgaccaggc tttcggtaaa
     4261 ctggccttcc ggcagctggg caatgatggt ttcgcggatg cgctggcttt cggttttgtc
     4321 ggctggcacg ctggcgggca cggacgccgt taccgggatc gattcatcac attcggtgat
     4381 ggtaaaaccc agtagatccg ccacttcacg ggcttccggg gtaataatgc tggcgcgcag
     4441 aaccacggac attgcctgtt cgccgcgcgc gtgtgcttca cgaatatcat tcgctgtgat
     4501 gagttttttc acctgcttcc tcctgtttgg tgagagatgc cagataatcc accagttgct
     4561 gcacgctttg cgggtcgtgg ctgtttagct cgaaaatcgg ctcttcgaaa cccgtctcaa
     4621 gcaataactt tcgcgttgcg gcgacgtcag catctggcat gtccgtttta ctgatgacgg
     4681 cgatttgccg tttgctgacg ccaatatcca gcaacccggc aggcaaccga ctttccgggt
     4741 catttgcgcc atggacatag atcagcatgt ctacatcctg tagcgtggta attaaggcgt
     4801 gataccagcg gggatggcta aaatattcac ccggcgtatc aatatcgccc ttatcattaa
     4861 attccacggc ctgtgttttt ctggcgaggg tataattccc ctgcaacgca ttaaataacg
     4921 ttgtttttcc tgccccgacg gtacccacaa acgcaattcg tttcatgaat gcctcgtatt
     4981 aacttttggt catttcacaa agcgtataat ttaataagcg acctaaaccg ctgacggttt
     5041 gtgataacgc ttcttctact gcgcccaccg agccataaat caccaatgca ccgctgaagc
     5101 ggtcgagaaa accgatatgc acatcagcgg ctttcagcgc taaatcaccg gcgatcatcg
     5161 ccgtttcgcc gggagttagc gtcataatgc caattgcgcc cgcatccgga acgccgattt
     5221 tcttcgccag ttcctcaccg ggatgcgcga tgagatgcgc cagtgtgacc tgtttgccgg
     5281 gcacaaattc ctgaatgatg cgttctttat ccatgagtcg ccgcctgtga aaaactttcg
     5341 tgtatcgtga caatttttcg tcagagtaaa taacaaaatt cggcaagcgc gtttaaaagg
     5401 tgaggtagat cactaagaat ggagagatat tcgctgtggt gcataaactt tcatatgcaa
     5461 cgcatgaata taaataaaat aatatcgaga tattatatag actgcttttt ttattattcg
     5521 caaactcagt aataagcttt actaatatat tgcagccaaa ccgcttaccc tgaatattca
     5581 gggtaagcgt gagagttaaa aaagaattac agcggttggg tttgcgcttc aaccacggcc
     5641 agcgccacca tgttgacgat acgacgcacc gatgcgatcg gcgttaacac gtgaaccggt
     5701 ttcgcaacgc ccatcagcac cggaccgaca gtcacacctt ccgagctgga aacgcgcagt
     5761 aagttgtaac taatgcgggc agcttccatg ttcggcatca ccagaatatt ggcagaacct
     5821 ttcaaagggc tgtccggcat acggtcgttg cgaatcgctt ccaccagcgc tgcatcgccg
     5881 tgcatttcac catcaatcat cagatctggc gcacgatccc tgaccagttc cagcgcctga
     5941 cgcattttgc tcgacgacgg gcagtcagaa gaaccaaagt tggagtgcga caacagagca
     6001 acgcgcggct caataccaaa acgacggaca gtttctgccg ccatcaaggt gatctccgcc
     6061 agctcttctg cgtctggttc gtcattgacg taggtatcgg caataaaggt gttaccactc
     6121 ggcagcagca gcgcgttcat tgcacctgcg gtgtgaacgc catcgcgata accaaagaca
     6181 tttttcacca cgctaaaatg ttcatgataa tcgcccaccg taccgcagat cattgcatcg
     6241 gcttccccac gctgaaccat gatcgcgccg atcactgtcg ggttactgat cagcgcccgc
     6301 tgcgcctgtt cctgagtgac accgcgacgc ttcatgatct ggaagtattc ggtccagtac
     6361 tctttaaagc gcggatcgga ttcgttattg acgatctcaa aatcaacgcc cgctttgatc
     6421 tgcaagccca gtttctgaat gcgcatttcg atcacgttcg gacgaccgat aaggatcggt
     6481 ttcgccagtc ccagcgttac cagttcctga gtggcatgca gaacgcgcgc ctcttcccct
     6541 tctgacagaa caacgcgctt cggcgctttg cgagcctggg agaaaatcgg cttcataaac
     6601 aggttggttt tgtaaacgaa ctcagtcagc ttgtcgatgt agacgtcgaa atcagcaatc
     6661 ggacgcgtcg ccacgcccga ctccatcgcg gctttagcga ccgcaggagc gatcttaacg
     6721 atcaagcgcg gatcaaacgg ttttggaatg atgtattccg gaccaaagct cagatcctga
     6781 tcgccatacg ctgaagccac cacttcgctc tgttcggcgt gggccagttc cgcaatcgcg
     6841 cgtaccgccg ccagtttcat ctcttcattg atagccgttg cgccaacatc cagcgcgcca
     6901 cggaagataa acgggaagca aaggacgttg ttgacctggt ttggatagtc agaacgaccg
     6961 gtgcaaatga tggcatccgg acgcacttct ttcgccagcg gcggcagaat ttccggttcc
     7021 gggttcgcca gcgccaggat cattggcgca cgagccattt tcttcaccat ttcctgggtc
     7081 agcactttcg ggccggaaca tccaaggaaa atatccgcgc cttcaatcac atcatcgagg
     7141 gtacgtttgc cgtcatccac caccgcatat gcggctttgg tttccgccat gtttggctca
     7201 cgcccctgat agataacgcc ttttgaatcg caaaccacga tgttatgttt ttgcagaccc
     7261 agcgctacca gcaggttcat acaggcgatt gctgcggcac ccgcgccgga aaccaccatc
     7321 cgcacgtcgg agatgttttt ctccaccacg cgcaagccgt tgaggatggc ggcagtgctg
     7381 atgattgccg tgccgtgctg atcgtcgtgg aataccggaa tattcatccg ctcgcgcagt
     7441 ttctgttcaa tatagaaaca ttctggcgct ttaatgtctt cgaggttgat tccgccgaag
     7501 gtcggttcga gcgcagcgac aacttcaata aatttgtccg ggtcgagttc atcaacttca
     7561 atgtcaaata catcaatccc ggcgaatttc ttaaacagaa cgcccttgcc ttccatcacc
     7621 ggtttgccag ccagcgcgcc aatgttgcct aaccccagca ccgccgtacc gttagagatc
     7681 accgccacca ggttaccgcg cgcggtatat ttgtaggctt ttaacgggtc tttttcgatt
     7741 tcaagacaag gtgcggcaac gcctggtgag taggccagcg ccagatcgcg ctgcgttgcc
     7801 agaggcttgg ttggagaaac ctggattttc cctggaactg gaaattcatg gaaatcaagt
     7861 gcactttgtt ttaactggtc atccatttgg ttgttccttt cacgtaacgt tcacaaataa
     7921 agtgtgtggg caacagcccc tgcccacaac gtggcgcaca ttattaccct gccggagtct
     7981 acagactttg agcaagtcca aactctcacc attaatataa tgttttggta ataatcctat
     8041 aacactgatg ttacctgctt aatccagcaa taccatgcct gtctgctatg cttttttgat
     8101 gcgtttagcg aaatttctca gaagtgtgaa ttaacgcact catctaacac tttacttttc
     8161 aaggagtatt tcctatgaac gagttagacg gcatcaaaca gttcaccact gtcgtggcag
     8221 acagcggcga tattgagtcc attcgccatt atcatcccca ggatgccacc accaatcctt
     8281 cgctgttact caaagctgcc ggattatcac aatatgagca tttaatagac gatgctatcg
     8341 cctggggtaa aaaaaatggc aagacccagg aacaacaggt ggtcgcagcg tgtgacaaac
     8401 tggcagtcaa tttcggtgct gaaattctca aaatcgtacc cggtcgcgtg tcaacagaag
     8461 ttgatgcacg cctctctttt gataaagaaa agagtattga aaaggcgcgc catctggtgg
     8521 acttgtatca gcaacaaggc gttgagaaat cacgcatttt gatcaaactg gcttcgacct
     8581 gggaaggaat tcgcgcggcc gaagagctgg aaaaagaagg tattaactgc aacctgacgc
     8641 tgctgttttc ttttgcccag gcgcgggcct gtgcagaagc gggcgttttt ctgatttcgc
     8701 cgtttgtcgg gcgtatttat gactggtatc aggcacgcaa gccgatggac ccgtatgtgg
     8761 tggaagaaga tccgggcgtt aaatcggtgc gcaatatcta cgactactat aagcaacacc
     8821 actatgaaac cattgtgatg ggcgcgagct tccgtcgcac cgaacaaatc ctcgccttaa
     8881 ccggctgcga tcgactgact atcgcaccga atttgctgaa ggagctgcag gaaaaagttt
     8941 cgccagtggt acgtaaatta atcccgcctt ctcagacgtt cccacgccca gcacccatga
     9001 gcgaagcgga gttccgttgg gagcacaatc aggatgcgat ggcggtagaa aaactgtctg
     9061 aaggcattcg tctgttcgct gttgatcaac gcaaactgga agatcttctt gccgccaaac
     9121 tataaaccag ccacggagtg ttatatgtcc cgaaaagacc ttgccaatgc gattcgcgca
     9181 ctcagtatgg atgcggtaca aaaagccaat tctggtcatc ccggcgcgcc gatgggcatg
     9241 gctgatattg ccgaagtgct gtggaacgat tttcttaaac ataatcctac cgacccaacc
     9301 tggtatgatc gcgaccgctt tattctttcc aacggtcacg cgtcgatgct gctctacagt
     9361 ttgctacatc tgaccggtta cgacctgccg ctggaagaac tgaagaactt ccgtcagttg
     9421 cattcgaaaa ccccaggcca cccggagatt ggctatacgc caggcgttga aaccaccacc
     9481 ggcccgcttg gacaaggttt ggcgaacgcc gtcgggctgg cgatagcaga gcgtacactg
     9541 gcggcgcagt ttaaccagcc agaccatgag atcgtcgatc acttcaccta tgtgtttatg
     9601 ggcgacggct gcctgatgga aggtatttcc cacgaagtct gttcgctggc aggcacgctg
     9661 ggactgggca agctgattgg tttttacgat cacaacggta tttccatcga cggtgaaaca
     9721 gaaggctggt ttaccgacga tacggcaaaa cgttttgaag cctatcactg gcatgtgatc
     9781 catgaaatcg acggtcacga tccgcaggcg gtgaaggaag cgatccttga agcgcaaagc
     9841 atgaaagata agccgtcgct gattatctgc cgtacggtga ttggctttgg ttcgccgaat
     9901 aaagcaggta aggaagaggc gcacggcgca ccactggggg aagaagaagt ggcgctggca
     9961 cggcaaaaac tgggctggca ccatccgcca tttgagatcc ctaaaaagat ttatcacgcc
    10021 tgggatgccc gcgaaaaagg cgaaaaagcg cagcagagct ggaatgagaa gtttgccgcc
    10081 tataaaaagg ctcatccgca actggcagaa gagtttaccc gtcggatgag cggtggttta
    10141 ccgaaggact gggagaaaac gactcagaaa tatatcaatg agttgcaggc gaatccggcg
    10201 aaaatcgcca cccgtaaggc ttcgcaaaat acgcttaacg cttacggacc gatgctaccg
    10261 gagctgctcg gaggttcagc ggatctggct cccagcaacc tcaccatctg gaaaggctct
    10321 gtgtcgctga aggaagatcc ggcgggcaac tacattcact acggggtgcg tgaatttggc
    10381 atgaccgcta tcgccaacgg cattgcgcac cacggcggct ttgtgccgta taccgcgacg
    10441 ttcctgatgt ttgttgaata cgcccgtaat gccgcgcgga tggcggcact gatgaaagca
    10501 cggcagatta tggtttatac ccacgattct attggcttag gggaagatgg tccgacgcac
    10561 caggccgttg agcaactggc cagtctgcgc ttaacgccga atttcagcac ctggcgaccg
    10621 tgcgatcagg tggaagcggc ggtgggctgg aagctggcgg ttgagcgcca caacgggccg
    10681 acagcactga tcctctcaag gcagaatctg gcccaggtgg aacgtacgcc ggatcaggtt
    10741 aaagagattg ctcgtggcgg ttatgtgctg aaagacagcg gcggtaagcc agatattatt
    10801 ctgattgcca ccggttcaga gatggaaatt accctgcaag cggcagagaa attagcagga
    10861 gaaggtcgca atgtacgcgt agtttccctg ccctcgaccg atattttcga cgcccaggat
    10921 gaggaatatc gggagtcggt gttgccttct aacgttgcgg ctcgcgtggc ggtggaagca
    10981 ggtattgccg attactggta caagtatgtt ggtctgaaag gggtaattgt cgggatgacg
    11041 ggttacgggg aatctgctcc ggcggataag ctgttcccgt tctttggctt taccgccgag
    11101 aatattgtgg caaaagcgca taaggtgctg ggagtgaaag gtgcctgatg gtgatcgccg
    11161 gatgctgatt gccggatgcg acgctgacgc gtcttatccg gcctacagcg tcttatccgg
    11221 cctacatgtc cccgcaattt tgtttaacgg gtgatccaca acgtgggcca ggcgtctggc
    11281 ccatgccagt tatcgcaggt gggttctgcg gcataacgca ccaggcgaaa acgctgaccg
    11341 tcaaagcgcc agcgcgcctg aatgccacaa tcgcttaatc cgcgcccttt cgctaaggtc
    11401 accagttcac gcgatttctc atcaaatgtt gcgttcatca gttccagttc attcgtctcc
    11461 tgaccgttgt tgaatggcaa acgcaaccga accgggcgcg aggctagtgg ctttttacgc
    11521 gacacaatcc atgccaaatc aatggtgtta taggcccctg cctcacagct aatcatcatc
    11581 agcgctttat catcagtcag cgcagtgaca ttcacctcac gacgtaacgg atcaagcgag
    11641 cagcgcagac cattcatccg ccagtttcca taatccagca aatcgttgcg ttcttcgagt
    11701 gagagtggcg tcggcgttgg gttaaccacc gcgacctctt tcagcgcagg cgcgggcggt
    11761 acgctgagcg gcggttcgtc ccctttcttg atccacgcgg tttcactgcc aacgcgcttt
    11821 tgctgagcat caataaacaa caacgccgct tttaagccac tcagagaaat ggtctgattg
    11881 ccgtcgcgta aggtgatcgc cttcccttcc tgaatcacct gcaaaaacgc ggtgatggtt
    11941 gccgtatcat cggttactaa taaccatggt gaaatccgcc acttgtcacc acttaacgcc
    12001 aacggctcac catctaacag cagccgtggc gctatctctc cttcagacgc atccggcgac
    12061 ttcaacccgc cgcgctcaat acgtaaaacg gcatcggtat gcgccccggc gctgcggctc
    12121 agggtcatca ccagtccatt atgatcgccc gtattgcgcg ccacgcagaa gttttggtta
    12181 ttacaggtga cctgccagtc ggaaaacgcc cgttgcgctg gtgccgccca caccaaagac
    12241 gtcggcaaca aagcgaaaaa aaagagaagg aaaatgcgat agcgcatgga cggtacgacc
    12301 ccagaagttc aaacaaacag cacaagtcgt atcttcgttg cgcaggtagc gcggctcaat
    12361 cagatttgtc ggattgagcc aataataaac aacgaaatat tattgttatt tttcagtcca
    12421 ttaaatgtga cgtttgcaaa tagttaagca ataacaccgt cttaccgtca cgtatctcgc
    12481 cggttttgat catctccagc gcctggctga acggcagctc gagcacttca atatcttcat
    12541 cttcaacacc gccaccggcg ttagcgcgct gattgtcact gtattcggcg ataaaaaagt
    12601 ggattagctc agtcacaccg ccaggcgaca tatacagttc aaataatttg cgcacttcgc
    12661 ccacttcata gcccgtctct tcaatcgctt ctttacgaat gcacacttcc ggttcgtcgt
    12721 tatccagcag cccggcgcag gtttcaatca gctgcccgct ttcattgcca ttaacccagg
    12781 tagcgacacg gaactgacga atcagaacca cgctcttttt cttcgcgttg tacaggagga
    12841 tcgtcgcacc attaccgcga tcgtaaactt cacgtttatg gcggataact tcgccatctt
    12901 tgcgcgtgag atcgtaggta atgttgtgca gggtgaaata gttatcggag agaattttgt
    12961 ctttaatgag ggtgatttgt tgcgtcatac cgactccaca gcgcgaaatg aacaattatc
    13021 ttacgctgtg aagtcgggtt tgtctgcgca ggctatcagt gagatttgac tgattttacc
    13081 cccagccagt caataatccc ctgtgccgca tgacgtcctt ctgccattgc ggtaaccacc
    13141 agatccgcac cacgcacggc gtcgccaccg gcgaagattt tcggattggt ggtctggtaa
    13201 cggtactggc tttccacatc cgcgatgatg cggccccatt tgtctaccgt tacaccgtgc
    13261 gactccagcc acggcatccc gtgtggattg aagccaaacg ccataatcac cgcatcggct
    13321 ggcatgacaa attcactgcc ctcaatcggt acaggtcgcc gacgcccctg ggcatccggc
    13381 tcgccaagac gcgtgcgcag gaaacgaatc ccgcagacgt gaccttgttc attcagctca
    13441 agcgccaccg gctggacgtt aaattcgaag ttggccccct cttcgcgggc gttcctcacc
    13501 tctttcttcg agcctggcat gttagcttca tcacgacgat aagcgcaggt gacgttactc
    13561 gcgccgtggc gcagcgcagt acgcacacag tccatcgcgg tgtcgccgcc gcccagtacc
    13621 acgacgttaa gtccggcggt attgataaac ggctcttccg gtagctcttc gagccccatc
    13681 acctgtttag tgttggcaat gaggaacggc agcgcgtcat aaacgcccgg cgcatcttca
    13741 ttgggtaaac ccgcgttcat ggaacggtaa gtgcctacgc caacgaagac cgcgtcgtat
    13801 tgttccaaaa gcgaatccaa agagacatct ttacccactt cacaattgag ttcgaagtga
    13861 atccccatcg cgctgaagat ttcccgacgg cgtgccagca gggatttatc cagtttgaaa
    13921 gaaggaatgc cgaaagtgag caagccaccg atttctggat ggcgatcgta caccgtcacc
    13981 gccacgccat tgcgggtcag aacatccgca caggccagcc ctgccggacc tgccccgata
    14041 atcgccaccc gcttgtccac tttggtgaca tgacttaagt caggacgcca acctttcgcc
    14101 aacgcctgat ctgaaatata gcgttcaata ttgccgatag ttaccgcgcc gtgctcatcg
    14161 cgaatagtac aggcaccttc acacaaacgg tcttgcggac aaacgcgtcc ggtaatttcc
    14221 ggcagggtgt tggtctggtg agaaagctcg acggcggcgt cgatgtttcc ggctttcacc
    14281 agttcaatcc actgcggaat atggttatgc agcgggcagg tccattcaca aacgctgtgc
    14341 tcgccgcact taaggcagcg cgaggcttcc cgttgtgcct ggtcggcgcg aaatggcaga
    14401 taaatttcat caaaaccggt tttgcgcgct tcaatcgcca gtttatccgg ctcgccacgc
    14461 gcgggcgttg cctgcatttg ttcgacttta ctcattaccg gcatttcttg cgccgcggta
    14521 ctggcatgcc acggttgatg ttcctgacgc gcggtgcgca agcggcgggg tttcgccatg
    14581 ccggagagtg cgacgtcagt gaccagttgc agcgcgtccg ccgggcaatt ctcaacacag
    14641 gcaggaccgt tttcgcgccc cgcacaaagg tcgcatttat gcgccgtggc ttttactttt
    14701 cctgccgcga cgggtgtcag gacgatttgc atcgtaccaa aaggacaggc caccacgcag
    14761 gatttacagc caatacactt ttgctgattg acctgaatgc tgtcatcaac gtggctgatt
    14821 gcgccattag ggcagctacg ggcgcagggc gcatcttcac aatggtgaca ggtcactgca
    14881 ctacgttgct gttgatgttt gataaccgta attcggggat gaaaatggtg ttggctcagg
    14941 acatgttgct catcattgtg agccatgaca caggcgattt cacaagcatg acaacccaga
    15001 cattgctgac tgttggccat aataaaacga ttcataacga ccttcttttt tggttgtaaa
    15061 aaccttattc tttatatgag tgttgttatt acccgactta caggggaatc ggcaatgttc
    15121 atgtgcccag aataagtaac tatttcgcta taaactgtgg cagatcaaat aatcccatca
    15181 ctgactaaat tgtgtttcag ctaactggaa cattaatgat tttttgtgga gaagacgcgt
    15241 gtgattgtta aacgacccgt ctcggccagt ctggcccggg ccttttttta cattgtgctg
    15301 ctgtcgattc tttccacggg tatcgctctg ctaactctgg cgagcagttt gcgcgacgct
    15361 gaggctatca atattgccgg atcgctacgt atgcagagtt accgcctggg ctacgacttg
    15421 caaagtggca gtccacaact caatgcacat cgccagttgt ttcagcaggc actgcattca
    15481 ccggtattaa ccaatctcaa cgtctggtat gtgccagaag cagtaaaaac tcgctatgcg
    15541 catctgaatg ccaactggct ggagatgaat aatcggctca gcaagggcga tttgccgtgg
    15601 tatcaggcca atattaataa ttatgttaat cagatagacc tgttcgtgct ggctttacag
    15661 cactacgctg aacgcaaaat gctgctggtg gtggcgattt ccctggctgg cggtatcggt
    15721 attttcacgc tggtcttttt tactttgcgc cgtatacgcc atcaggtggt tgccccgctg
    15781 aatcagctgg ttaccgccag tcaacgtatt gaacacgggc agttcgactc gccgccgctg
    15841 gataccagtc tgccgaatga gcttggtctg cttgcaaaaa cctttaacca gatgtcgagc
    15901 gagctgcata aattgtacct ttcgctggag gcgtcagtag aagaaaagac ccgcgatctc
    15961 cacgaggcca agcgtcgtct ggaggtgttg tatcaatgtt cccaggcgct gaacaccagc
    16021 cagattgatg tgcattgttt ccgccatatt ttgcagattg ttcgcgacaa tgaagcggct
    16081 gaatatctgg agttaaatgt cggtgaaaac tggcggatta gcgaagggca accaaacccg
    16141 gaattgccga tgcagatttt accggtgaca atgcaagaga cggtttacgg cgaactgcac
    16201 tggcaaaata gtcacgtttc atcatcagaa ccgctgctta acagtgtttc atcgatgctg
    16261 ggacgcggtt tgtactttaa tcaggcgcag aagcattttc agcagttatt gttgatggaa
    16321 gaacgtgcaa ccatcgcccg cgaattgcac gactcgctgg ctcaggtact ttcttactta
    16381 cgtatccagt tgacgttact gaagcgttcg ataccggaag ataacgccac cgcacaaagt
    16441 atcatggccg atttttccca ggcgttgaat gatgcttatc ggcagttacg cgagctgttg
    16501 actactttcc gcctgacgct gcagcaggcg gatctcccct ccgcgttgag ggaaatgctg
    16561 gatacgttac aaaatcaaac cagcgccaaa ctgaccctcg actgccgtct gccaaccctg
    16621 gcgctggatg cgcaaatgca ggtgcatttg ttgcaaatta ttcgcgaagc ggtgctgaat
    16681 gcgatgaagc acgccaacgc cagcgaaatc gccgtcagtt gcgtcaccgc gccggacggc
    16741 aatcacacgg tttatatccg tgataacggg attggtatcg gtgaaccgaa agaacccgaa
    16801 ggtcattatg gtctgaatat catgcgcgaa cgcgcggaac ggctgggtgg gacgctgact
    16861 ttttcacaac cttccggcgg cggcacgtta gtgagtatta gctttcgctc tgcggagggt
    16921 gaggaaagtc agttgatgta atgcctccta ctgaccaaag aatacttgca cttaaggttc
    16981 agtataaaag ggcatgataa tttacattaa ctcctttttt tctccacgat tggctcgtac
    17041 cttgccgcta cagtgaagca agtcaagcct acaacgatac gcagaaacac gaggtcctct
    17101 tttaatggcg aatttcttta ttgatcgccc catttttgcc tgggtgctgg caatcctgtt
    17161 gtgtctgaca gggaccctgg cgattttttc attgcccgtt gaacaatacc ccgatctcgc
    17221 gccaccgaat gtgcgagtga ccgctaacta tcccggcgca tcggcccaga cgctggaaaa
    17281 caccgtgacc caggttatcg agcaaaatat gaccggcctc gataatctca tgtatatgtc
    17341 atctcagagc agtggcaccg gtcaggcatc tgtcacttta agttttaaag caggcaccga
    17401 tccggacgaa gccgtgcagc aagtacaaaa ccagctgcaa tcagccatgc gaaagttacc
    17461 gcaggcggtg caaaatcagg gcgtgacggt gcgtaaaacc ggcgatacca atattctgac
    17521 cattgctttc gtctctaccg atggttcgat ggataaacag gatattgccg attatgttgc
    17581 cagtaatatt caggacccgt taagccgcgt gaatggcgtc ggggatatcg atgcctatgg
    17641 ttcgcaatat tccatgcgta tctggctgga cccggcgaaa ctcaacagtt tccagatgac
    17701 ggctaaagat gtcactgatg ccattgagtc acagaacgcg cagattgcgg ttgggcaact
    17761 tggtggtaca ccttccgtcg ataagcaggc gctcaacgcc accattaacg cccagtcact
    17821 gctgcaaaca ccagaacagt tccgcgatat caccttgcgg gtgaatcaag acggctcaga
    17881 ggtaaggctg ggcgatgtcg ccaccgtcga aatgggggcg gagaaatacg attatcttag
    17941 ccgcttcaat ggtaagccag cctccgggct gggggtaaaa ctggcctccg gcgcaaacga
    18001 aatggcgaca gcggagctgg tgctcaatcg tctcgacgag ctggcgcagt acttcccgca
    18061 cggactggaa tacaaggtgg cgtatgaaac cacctcgttt gttaaagcct ccattgaaga
    18121 cgtggtgaaa acgctgctgg aagctatcgc cctggttttc ctcgttatgt atctgttcct
    18181 gcaaaacttc cgcgccacgc tgatacccac tatcgccgtg ccggtggtgt tgatgggaac
    18241 cttctccgta ctttacgcct tcggttacag cgtcaacacc ttaaccatgt tcgcgatggt
    18301 actggcgatc ggtctgctgg tggatgacgc aatcgtggtg gtggaaaacg tcgaacgtat
    18361 tatgagtgag gaaggactca ctcctcgcga agccacacgt aaatcgatgg ggcagatcca
    18421 gggggcactg gtcgggattg cgatggttct ttcggcggta tttgtaccaa tggccttctt
    18481 cggcggcacc accggtgcca tctatcgcca gttctctatt accattgttg cggcgatggt
    18541 gctgtcagta ctggtagcga tgatcctcac tccggctctg tgtgccacac tacttaagcc
    18601 actgaaaaaa ggtgagcatc acgggcaaaa aggctttttt gcctggttta accagatgtt
    18661 taaccgcaac gccgaacgct acgaaaaagg ggtggcgaaa attctccacc gtagcctgcg
    18721 ctggattgtg atttatgtcc tgctgcttgg cggcatggtg ttcctgttcc tgcgtttgcc
    18781 gacgtcgttc ttaccgcagg aagaccgtgg catgtttact acctcggtac aattgcccag
    18841 cggttcaacc caacaacaga ccctgaaagt cgttgagcaa atcgagaaat attacttcac
    18901 ccatgaaaaa gacaacatca tgtcggtgtt tgccactgtt ggttctggcc ccgggggtaa
    18961 cgggcaaaac gtggcgcgaa tgtttatccg cctgaaagac tggagcgaac gcgacagtaa
    19021 gaccggcacc tcgtttgcca ttatcgagcg tgcaacgaag gcgtttaacc aaattaaaga
    19081 agctcgcgtt atcgccagca gcccgccagc aattagcggt cttggtagtt ctgcaggttt
    19141 tgatatggag ttgcaggacc acgctggagc gggtcacgat gcgctgatgg cagcacgtaa
    19201 tcagttgctg gcgctggcgg cggaaaaccc ggagctaacc cgtgtgcgcc ataacggcct
    19261 cgacgacagt ccgcagttgc agattgatat cgaccagcgt aaagctcagg cgctgggcgt
    19321 tgctatcgac gatattaacg acacactgca aaccgcctgg ggttcgagct atgtgaatga
    19381 ctttatggat cgcggtcgcg tgaagaaagt ctatgtgcag gcagctgcgc cgtatcgcat
    19441 gctgccagat gacattaatc tctggtatgt ccgaaataaa gatggcggca tggtgccctt
    19501 ctctgctttc gcgacctcac gctgggaaac aggctcgccg cgtctggaac gctataacgg
    19561 ttattctgcg gttgagattg ttggggaagc cgcaccgggg gtcagtaccg gtacggcgat
    19621 ggatattatg gaatcgttag tgaagcagtt gccaaacggc tttggtctgg agtggacggc
    19681 gatgtcgtat caggagcgac tttccggcgc gcaggctccg gcgctgtacg ccatttcctt
    19741 gctggtggta ttcctgtgtc tggcggcgtt gtatgaaagc tggtcggtgc cgttctcggt
    19801 aatgctggtc gtgccgctgg gggttatcgg cgcgctgctg gcaacctgga tgcgcgggct
    19861 ggaaaacgac gtttacttcc aggtgggcct gttaacggtc attggtttat cggcgaaaaa
    19921 cgccatcctg atcgtcgagt ttgctaacga gatgaaccaa aaaggccacg acctgtttga
    19981 agcgacgctc cacgcctgcc gtcagcgttt acgcccgatt ctgatgacct cgctggcatt
    20041 tatcttcggc gtattgccaa tggcaaccag cacgggtgcc ggttccggtg gtcagcatgc
    20101 ggtgggtact ggcgtaatgg gcgggatgat ttcagctact attctggcta tttacttcgt
    20161 gccgctgttc tttgtgctgg tgcgccgccg cttcccgctg aagccgcgcc cggaataagc
    20221 aataaaaaag gcgacatgcc aatgtgtcgc ctttttcaac tttccgatta agaacctgct
    20281 cagcgggttc ttgctgttta tactttgtct cgggaattac ttacgaagca taacttcgat
    20341 aaagtctttc cagttcccca gttcacgttc aatcataaca acctctctta taattatggg
    20401 tattctacgg aaacaatata ccgtggtgaa gctaatttac tcgattgctg cgatgactac
    20461 ctccggggga caaaccttat gtaaatacta tggtcctgcg gtgataattt gtatgtgata
    20521 cacagcaaca tttcgaga
//
LOCUS       DAZKHF020000065        20378 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2201S31241
            SAMN43267800-rid25106413.denovo.065, whole genome shotgun sequence.
ACCESSION   DAZKHF020000065 DAZKHF020000000
VERSION     DAZKHF020000065.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267800
            Sequence Read Archive: SRR30307263
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 20378)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20378)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 20378)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350581.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350581.2
            Genome Coverage       :: 151x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:21:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,652
            CDSs (total)                      :: 5,557
            Genes (coding)                    :: 5,320
            CDSs (with protein)               :: 5,320
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 237
            CDSs (without protein)            :: 237
            Pseudo Genes (ambiguous residues) :: 0 of 237
            Pseudo Genes (frameshifted)       :: 102 of 237
            Pseudo Genes (incomplete)         :: 130 of 237
            Pseudo Genes (internal stop)      :: 52 of 237
            Pseudo Genes (multiple problems)  :: 41 of 237
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20378
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267800-rid25106413.denovo.065"
                     /serotype="O 26:H11"
                     /isolate="2201S31241"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-01"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            <1..882
                     /locus_tag="ACHOSC_RS09625"
                     /old_locus_tag="pgaptmp_001925"
     CDS             <1..882
                     /locus_tag="ACHOSC_RS09625"
                     /old_locus_tag="pgaptmp_001925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HGU4547873.1"
                     /translation="LLDKLREQYGVGPVCSELHIAPSTYYHCQQQRHHPDKRSARAQR
                     DDWLKREIQRVYDENHQVYGVRKVWRQLLREGIRVARCTVARLMAVMGLAGVLRGKKV
                     RTTVSRKAVSVGDRVNRQFVAERPDQLWVADFTYVSTWQGFVCVAFIIDVFAGCIVGW
                     RVSSSMETTFVLDALEQALWARRPSGTVHHSDKGSQYVSLAYTERLKEAKLLASTGST
                     GDSYDNAMAESINGLYKAEVIHRKSWKNRAEVELATLTWVDWYNNRRLLGRLGHIPPA
                     EAEKAYYASIRNDDLAA"
     misc_feature    2..66
                     /locus_tag="ACHOSC_RS09625"
                     /old_locus_tag="pgaptmp_001925"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01497"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="AL1L pseudoknot; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /db_xref="RFAM:RF01497"
     gene            1055..3265
                     /gene="katP"
                     /locus_tag="ACHOSC_RS09630"
                     /old_locus_tag="pgaptmp_001926"
     CDS             1055..3265
                     /gene="katP"
                     /locus_tag="ACHOSC_RS09630"
                     /old_locus_tag="pgaptmp_001926"
                     /EC_number="1.11.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000592771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="catalase/peroxidase KatP"
                     /protein_id="HGU4547874.1"
                     /translation="MIKKTLPVLILLALSGSFSTAVAADKKETQNFYYPETLDLTPLR
                     LHSPESNPWGADFDYATRFQQLDMEALKKDIKDLLTTSQDWWPADYGHYGPFFIRMAW
                     HGAGTYRTYDGRGGASGGQQRFEPLNSWPDNVNLDKARRLLWPVKKKYGSSISWGDLM
                     VLTGNVALESMGFKTLGFAGGREDDWESDLVYWGPDNKPLADNRDKNGKLQKPLAATQ
                     MGLIYVNPEGPGGKPDPLASAKDIREAFSRMAMDDEETVALIAGGHTFGKAHGAASPE
                     KCIGAGPDGAPVEEQGLGWKNKCGTGNGKYTITSGLEGAWSTSPTQFTMQYLKNLYKY
                     EWELHKSPAGAYQWKPKKAANIVQDAHDPSVLHPLMMFTTDIALKVDPEYKKITTRFL
                     NDPKAFEQAFARAWFKLTHRDMGPAARYLGNEVPAESFIWQDPLPAADYTMIDGKDIK
                     SLKEQVMDLGIPASELIKTAWASASTFRVTDYRGGNNGARIRLQPEINWEVNEPEKLK
                     KVLASLTSLQREFNKKQSDGKKVSLADLIVLSGNAAIEDAARKAGVELEIPFTPGRTD
                     ASQEQTDVASFSVLEPTADGFRNYYSKSRSHISPVESLIDKASQLDLTVPEMTALLGG
                     LRVMDINTNNSSLGVFTDTPGVLDNKFFVNLLDMSTRWSKADKEDTYNGFDRKTGALK
                     WKASSVDLIFSSNPELRAVAEVYASDDARNKFIHDFVKSWNKVMNSDRFDLNNK"
     gene            3309..3698
                     /locus_tag="ACHOSC_RS09635"
                     /old_locus_tag="pgaptmp_001927"
     CDS             3309..3698
                     /locus_tag="ACHOSC_RS09635"
                     /old_locus_tag="pgaptmp_001927"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016242783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA];
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome b562"
                     /protein_id="HGU4547875.1"
                     /translation="MQKSNKVIISCGMALYFLCGGITVAEELDESMQVLARNMSVLQS
                     SMDKKELLEALDRMDDAVDESMKRLPESISPADEEGKNDYIKELQKLEKEIMLAKQKV
                     LSGQLSDIPDSVNKMNKIRVEGHDKFR"
     gene            3812..4579
                     /locus_tag="ACHOSC_RS09640"
                     /old_locus_tag="pgaptmp_001928"
                     /pseudo
     CDS             3812..4579
                     /locus_tag="ACHOSC_RS09640"
                     /old_locus_tag="pgaptmp_001928"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612086.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
     misc_feature    4092..4202
                     /locus_tag="ACHOSC_RS09640"
                     /old_locus_tag="pgaptmp_001928"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01497"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="AL1L pseudoknot; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /pseudo
                     /db_xref="RFAM:RF01497"
     gene            complement(4659..5054)
                     /locus_tag="ACHOSC_RS09645"
                     /old_locus_tag="pgaptmp_001929"
     CDS             complement(4659..5054)
                     /locus_tag="ACHOSC_RS09645"
                     /old_locus_tag="pgaptmp_001929"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid partitioning/stability family protein"
                     /protein_id="HGU4547876.1"
                     /translation="MESSDPKKRKKVVAYLHPTLYPQDNLTQQTIDSLPIQMRGDFYR
                     QSLICGAALYSVDPRLLTLISGFFSEKITAENLVKLIEQTTGYTSTSIDISVLKNIIG
                     ASSENKSESITSKDDFEEQTRRNLSMLKK"
     gene            complement(5054..6013)
                     /locus_tag="ACHOSC_RS09650"
                     /old_locus_tag="pgaptmp_001930"
     CDS             complement(5054..6013)
                     /locus_tag="ACHOSC_RS09650"
                     /old_locus_tag="pgaptmp_001930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_process: GO:0030541 - plasmid partitioning [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid segregation protein ParM"
                     /protein_id="HGU4547877.1"
                     /translation="MLKVSCDDGSTNVKLAWLEDGEVRTSLSGNSFKEGWNPGLFNAG
                     KVYNYVVDGKKYTYDLGSTAVIGTTHVSYQYSTTNLLAIHHALLTSGLQPQDVELTVT
                     LPVTEFFDNDNQPNEERIERKKANVLREISLNKGETFKIKKVNVMPESLPAAFESLKK
                     DKVNKLERSLIIDLGGTTLDCGLILGAFEGISEIRGYSEIGTSRITHTVMNALTKAST
                     PCNYFIADELIKNRHDNEYLQTLINDVAEIKNITHVIDREVKSLAESIRQEISTFSGM
                     NRIYLTGGGAELIYPHIKQYFPNLKVNKVDEPQFALVKAMVHA"
     gene            6286..7188
                     /locus_tag="ACHOSC_RS09655"
                     /old_locus_tag="pgaptmp_001931"
     CDS             6286..7188
                     /locus_tag="ACHOSC_RS09655"
                     /old_locus_tag="pgaptmp_001931"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1281 domain-containing protein"
                     /protein_id="HGU4547878.1"
                     /translation="MHFSGEPAQIAEIKRLASGAVTPLYRRATNEGIQLFLAGSAGLL
                     QTTEDVRFEPCPGLTAAGRGVVSPENIAFTRWLTYLQNGVLLDEQNCLMLHELWLQSG
                     TGQRRWEGLPDDVRDTITALFTAKRGDWCGFWSNEDVSVWWNRLCDNVLPEKTMPFDL
                     LTVLPTRLDVEVNGFNGGVLNGVPSAYHWYTEQYGVKWPVGYEVNISSQGDNFIQVDF
                     DTPWYQPESDVIAELSRRFSCTLEHWYAEQGCNFCGWQRYERGELVDVLWGELEWSHP
                     TDDDELPEVTGPAWIVDKVAHYGG"
     gene            7185..7496
                     /locus_tag="ACHOSC_RS09660"
                     /old_locus_tag="pgaptmp_001932"
     CDS             7185..7496
                     /locus_tag="ACHOSC_RS09660"
                     /old_locus_tag="pgaptmp_001932"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858330.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4547879.1"
                     /translation="MKYDGDGRATARFFSGKGCRRAPLFTAPADAARHKRCLWSVSRV
                     RRARDGRFYRSRLASVTVYASPSPFSDERPSSRFRGIALPSKRRRLRYSTVGLTRYRT
                     R"
     gene            7572..8255
                     /locus_tag="ACHOSC_RS09665"
                     /old_locus_tag="pgaptmp_001933"
     CDS             7572..8255
                     /locus_tag="ACHOSC_RS09665"
                     /old_locus_tag="pgaptmp_001933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858331.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA methylase"
                     /protein_id="HGU4547880.1"
                     /translation="MSRFVLGNCIDVMARIPDNAIDFILTDPPYLVGFRDRSGRTIAG
                     DVNDDWLQPASNEMYRVLKKDALMVSFYGWHRIDRFMAVWIRAGFSVAGHLVFTKNYT
                     SKSAYVAYRHECAYILAKGSPARPRNPLPDVLGWKYSGNRHHPTEKPVTSLQPLIESF
                     THPNSIVLDPFAGSGSTCVAALQSGRRYIGIELLEQYHRAGQQRLAAVQRAMQQGAAN
                     DNRFEPEAA"
     gene            8255..8473
                     /locus_tag="ACHOSC_RS09670"
                     /old_locus_tag="pgaptmp_001934"
     CDS             8255..8473
                     /locus_tag="ACHOSC_RS09670"
                     /old_locus_tag="pgaptmp_001934"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4547881.1"
                     /translation="MNYSGFEDMREQVAELANTMCDLRTTMNEMEQRYSDADTLPERL
                     VRQTLFRASHLFMKAYNEILELDYCFKD"
     gene            8433..8919
                     /locus_tag="ACHOSC_RS09675"
                     /old_locus_tag="pgaptmp_001935"
                     /pseudo
     CDS             8433..8919
                     /locus_tag="ACHOSC_RS09675"
                     /old_locus_tag="pgaptmp_001935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052646.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1380 family protein"
     gene            8964..9203
                     /locus_tag="ACHOSC_RS09680"
                     /old_locus_tag="pgaptmp_001936"
                     /pseudo
     CDS             8964..9203
                     /locus_tag="ACHOSC_RS09680"
                     /old_locus_tag="pgaptmp_001936"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858335.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            9198..9446
                     /gene="psiB"
                     /locus_tag="ACHOSC_RS09685"
                     /old_locus_tag="pgaptmp_001937"
                     /pseudo
     CDS             9198..9446
                     /gene="psiB"
                     /locus_tag="ACHOSC_RS09685"
                     /old_locus_tag="pgaptmp_001937"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000845953.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="conjugation system SOS inhibitor PsiB"
     gene            9443..10162
                     /locus_tag="ACHOSC_RS09690"
                     /old_locus_tag="pgaptmp_001938"
     CDS             9443..10162
                     /locus_tag="ACHOSC_RS09690"
                     /old_locus_tag="pgaptmp_001938"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001723065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid SOS inhibition protein A"
                     /protein_id="HGU4547882.1"
                     /translation="MSARSRALIPLSAEQQAAMQAVAVTEQRRRQGRTLSAWPYASAF
                     FRCLNGSRRISLTDLRFFAPALTKEEFHGNRLLWLAAVDKLIESFGEVCVLPLPSDAG
                     HRLFPSVPFREGERRRQKTTLTEQKYSRQREREAERRELEYQTCFAQAQIDLAFHTPA
                     TVGSWLSRWSGVVEEHDLETIFWGWCGRFPSLSSFDRFFWQEEPLWRLIFEAGEAGRG
                     APVQVRALEQWMIPNKLENAI"
     gene            10159..10756
                     /locus_tag="ACHOSC_RS09695"
                     /old_locus_tag="pgaptmp_001939"
                     /pseudo
     CDS             10159..10756
                     /locus_tag="ACHOSC_RS09695"
                     /old_locus_tag="pgaptmp_001939"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000978013.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            11218..11718
                     /locus_tag="ACHOSC_RS09700"
                     /old_locus_tag="pgaptmp_001940"
     CDS             11218..11718
                     /locus_tag="ACHOSC_RS09700"
                     /old_locus_tag="pgaptmp_001940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000117633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antirestriction protein ArdA"
                     /protein_id="HGU4547883.1"
                     /translation="MSVVAPAVYVGTWHKYNCGSIAGRWFDLTTFDDERDFFAACRAL
                     HQDEADPELMFQDYEGFPVNMASECHINWAWVEGFRQARDEGCEEAYRLWVDDTGETD
                     FDTFRDAWWGEADSEEAFAVEFVSDTGLLADVPETVALYFDYEAYARDLFLDSFTFID
                     GHVFRR"
     gene            12446..12880
                     /locus_tag="ACHOSC_RS09705"
                     /old_locus_tag="pgaptmp_001941"
     CDS             12446..12880
                     /locus_tag="ACHOSC_RS09705"
                     /old_locus_tag="pgaptmp_001941"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000218847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="post-segregation killing protein PndC"
                     /protein_id="HGU4547884.1"
                     /translation="MTVSSTISVFCRDGVFRTVYCHLHGEPTWNGRILHTHYATGQLA
                     EALVEHGDIRCLGPRCDKSAGHTLQNPVDGVTAYYGRDSGFRMDSEAREYRSFMEAIA
                     TESTEEVRFHYVFIDGYWKVMYRTPEGWKMKALALALRRCPK"
     gene            12973..13239
                     /locus_tag="ACHOSC_RS09710"
                     /old_locus_tag="pgaptmp_001942"
     CDS             12973..13239
                     /locus_tag="ACHOSC_RS09710"
                     /old_locus_tag="pgaptmp_001942"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001247860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4547885.1"
                     /translation="MRPSIIFATAEYVKRLREECLRENKPLHRHTRVRRQELAQDEIN
                     PDVLAMSGHIARRCSEQKRVRIPAMKVSEWGHLLRALEIERACH"
     gene            13304..14194
                     /locus_tag="ACHOSC_RS09715"
                     /old_locus_tag="pgaptmp_001943"
     CDS             13304..14194
                     /locus_tag="ACHOSC_RS09715"
                     /old_locus_tag="pgaptmp_001943"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000038335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rpn family recombination-promoting
                     nuclease/putative transposase"
                     /protein_id="HGU4547886.1"
                     /translation="MSKKKNTTTPTPHDAAFWSFLANPDVARDFLELHLPAEYRQLCD
                     LSTLKLEPATFVEPDLHQYASDILWSVKTTGGEDGYVYTLIEHQSTENLYMPFRMLRY
                     SVAAMQRHLEQHKTLPLVIPVLFYHGERSPYPYSMNWLDCFEEPALAAKIYTKPFPLV
                     DITVVDDNEIMNHRRMAALTLLMKHIRHRDMMELLDKLPQVMVEISDEQVRVLIHYIV
                     NAGDSVSPEFMRALAERLPQHEDKLMTIAERLEQKGRQEGALEKALAIACQLQKMGMT
                     PEQIKQATGLSEAELKNITH"
     gene            14194..14439
                     /locus_tag="ACHOSC_RS09720"
                     /old_locus_tag="pgaptmp_001944"
     CDS             14194..14439
                     /locus_tag="ACHOSC_RS09720"
                     /old_locus_tag="pgaptmp_001944"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014358130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4547887.1"
                     /translation="MRTTKPGKMPGFFVSRSRANSMTIAERLIQKGFDEGFKKGALEV
                     AREAACRLRDMGWTPERIQEATGLSGEELKKLFPDEQ"
     gene            complement(14470..14721)
                     /locus_tag="ACHOSC_RS09725"
                     /old_locus_tag="pgaptmp_001945"
     CDS             complement(14470..14721)
                     /locus_tag="ACHOSC_RS09725"
                     /old_locus_tag="pgaptmp_001945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003980153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XRE family transcriptional regulator"
                     /protein_id="HGU4547888.1"
                     /translation="MTGWELRIWRKSMLWSREKAAREFGVTQRTWHAWENAEQVDVTV
                     WRTTQALSVRDLLPHMQGMRKADIIRRLENELGETAGNV"
     gene            complement(14755..14950)
                     /locus_tag="ACHOSC_RS09730"
                     /old_locus_tag="pgaptmp_001946"
                     /pseudo
     CDS             complement(14755..14950)
                     /locus_tag="ACHOSC_RS09730"
                     /old_locus_tag="pgaptmp_001946"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001753860.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            15300..16148
                     /locus_tag="ACHOSC_RS09735"
                     /old_locus_tag="pgaptmp_001947"
     CDS             15300..16148
                     /locus_tag="ACHOSC_RS09735"
                     /old_locus_tag="pgaptmp_001947"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001401764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4942 domain-containing protein"
                     /protein_id="HGU4547889.1"
                     /translation="MNQTLPTADLNTAGTTDIIPSMAIDRIIAQRNEGIALFMQAMEC
                     LATARRILLDASGDIFLYGFEDCVTDSVRCMDKPEEAKKNITRLADRKIWDRLMTYTG
                     MYTFMSSCQRDEWNSQLMSDTCPEITLDNVLATFRHLNASKMQTFEQELIDIWRKLSW
                     DYRTNNPCRLGKRIIIENLLYRWSNGRVTLDCSGREALDDLVRPFYLLEGRNVPDFRN
                     SIGAQYGEFLGNGDNIGELFEGEYFTVRGYQKGTVHIVFKRPDLVEKLNDIIARHYPG
                     VLPPRV"
     gene            complement(16234..16569)
                     /locus_tag="ACHOSC_RS09740"
                     /old_locus_tag="pgaptmp_001948"
     CDS             complement(16234..16569)
                     /locus_tag="ACHOSC_RS09740"
                     /old_locus_tag="pgaptmp_001948"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000157082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-guanine dinucleotide biosynthesis
                     protein MobC"
                     /protein_id="HGU4547890.1"
                     /translation="MASKKFYSDDDIQLAKAALSELPDLTAQRKTLRDFLDAIRDDII
                     VLVRTKGYTLADVRDTLKNAGYEVGEKALRDIIREAESKKPSRRSSSKTAPKKTDSGR
                     KDGIDMNNN"
     gene            16801..17133
                     /gene="mobA"
                     /locus_tag="ACHOSC_RS09745"
                     /old_locus_tag="pgaptmp_001949"
     CDS             16801..17133
                     /gene="mobA"
                     /locus_tag="ACHOSC_RS09745"
                     /old_locus_tag="pgaptmp_001949"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001291057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid mobilization protein MobA"
                     /protein_id="HGU4547891.1"
                     /translation="MSEKKTRSGSEKRQKNVLIAVRFSPEEAEIVKEKAEKNGLTVST
                     LIRKTVLGKQINARIDEDFLKELMRLGRLQKHLFVEGKRTGDKEYAEVLVAITELANT
                     LRRDLMGR"
     gene            17145..19865
                     /locus_tag="ACHOSC_RS09750"
                     /old_locus_tag="pgaptmp_001950"
     CDS             17145..19865
                     /locus_tag="ACHOSC_RS09750"
                     /old_locus_tag="pgaptmp_001950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000991381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /protein_id="HGU4547892.1"
                     /translation="MNAIIPKKRRDGKSSFEDLIAYTSVRDDVPEDELRPDTEMKAEI
                     PHRNRFRRLVDYATRLRNEKFVSLIDVMKDGSQWVNFYGVTCFHNCLSLESAAEEMQK
                     AADLAHFSKDDTDPVFHYILSWPAHESPRSEQLFDCVRHTLKSLELSKHQYVAAVHTD
                     TDNLHVHVAVNRVHPETGYINCLPWSQEKLSRACRELEIKHGFAPDNGCFVHAPGNRI
                     VRKTALVRERRNAWRRGKKQTFREYIVQMSIAGLREEPAQDWLSLHKRLASDGLYITM
                     QEGELVVKDGWDRAREGVALSSFGPLWTAEKLGRKLGEYQPVPTDIFSQVGTPGRYDP
                     EAINVDIRPEKVAETESLKQYACRHFAERLPEMARNGELESCLDVHRTLATAGLWMGI
                     QHGHLVLHDGFDKQQTPVRADSVWPLMTLDYMQDLDGGWQPVPKDIFTQVIPGERFRG
                     RNLGTQAVSDYEWYRMRMGTGPQGAIKRELFSDKESLWGYGLIQCRSLIEEMIADGDF
                     SWQACHEMFARKGLMLQKQHHGLVIVDAFNHELTPVKASSIHPDLTLSRAEPQAGPFE
                     IAAADIFERVKPECRYNPELAASDEVEPGFRRDPEIRRERREARAAAREDLRARYLAW
                     KEHWRKPDLRYGERLREIHAACRRRKAYIRVQFRDPQLRKLHYHIAEVQRMQALIRLK
                     ESVKEERLSLIAEGKWYPLSYRQWVEQQAAQGDRAAVSQLRGWDYRDRRSRNKDKRRT
                     TNVDRCVVLCEPGGTPLFNNVAKLEARLQKNGSVHFRDTRTGKNVCTDYGDRVVFYHH
                     TDRNELAEKLDLIAPVLFSRNGKLGFEPEGSYQQFNDVFAEMVAWHNAAGITGNGHFT
                     ITRPDVDLHRQRSEQYYREYIRQQTRLSVSHDDNYTLRQEKTWEPPSPGM"
     gene            complement(20086..>20378)
                     /locus_tag="ACHOSC_RS09755"
                     /old_locus_tag="pgaptmp_001951"
     CDS             complement(20086..>20378)
                     /locus_tag="ACHOSC_RS09755"
                     /old_locus_tag="pgaptmp_001951"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000077650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="conjugal transfer protein TrbC"
                     /protein_id="HGU4547893.1"
                     /translation="QYDTDIESRANELWKKAVHTINNTTREERRVCYITLNRPEEEHS
                     DPEDIPSVKAILNTLLPLEILLPVAENNKSQTINNDHPQKSCGAQPTTDEKY"
BASE COUNT         4874 a         4874 c         5613 g         5017 t
ORIGIN      
        1 ctgctggata agctgcgtga gcagtacggg gtcggaccgg tatgcagtga actgcatatt
       61 gccccgtcaa cgtattacca ctgtcagcaa cagcgacatc atcctgataa acgcagtgcc
      121 cgtgctcagc gcgatgactg gctgaagaga gagatacagc gcgtatacga tgaaaatcat
      181 caggtgtacg gtgtgcgtaa agtctggcgc cagttgttac gcgaaggtat cagggtggcc
      241 agatgtacag tggcgcgcct catggcggtt atgggacttg ccggtgttct ccggggtaaa
      301 aaggtccgta ctaccgtcag ccggaaagcc gtttccgtag gcgaccgcgt aaaccgtcag
      361 ttcgtggcag aacgtcctga ccagctgtgg gtggctgatt ttacttacgt cagcacatgg
      421 cagggcttcg tctgtgtggc gttcatcatt gatgtgtttg ccggatgtat cgtggggtgg
      481 cgagtctcat cgtctatgga aacgacattc gtgctggatg cactggagca ggcgttgtgg
      541 gcccgtcggc cgtccggcac agtccatcac agtgataaag gttctcagta tgtatcactg
      601 gcctatacgg agcgactaaa agaagcaaaa ctgctggcat caacagggag tacaggtgac
      661 tcgtatgaca acgcgatggc ggagagcatc aatggtcttt acaaagcgga ggtaatacac
      721 cgtaagagct ggaaaaaccg tgcagaagtg gaactggcca cactcacgtg ggtggactgg
      781 tataacaatc gacgattgct gggaaggctg ggtcatatcc ctccggcaga agcagaaaaa
      841 gcttattatg cttccatcag aaacgatgat ctggcagcct gagttcacag ataaaacact
      901 ctccaggaaa accggggcgg ttcagttgcg gcttggaacc ccttagtatt ttttgtctgt
      961 agtatctatc ccagcaatag gtatatcctg ttgcatcaat aaagttgact tttgtataca
     1021 acatgcgaat ttcccttaat ccggagctat tcgtatgata aaaaaaactc ttcctgttct
     1081 gattcttctg gcgctatcgg ggagcttttc taccgctgta gccgctgata aaaaagagac
     1141 tcaaaatttc tactatccag aaacactgga tttaactcct ctgagattac acagccctga
     1201 atcaaatccc tggggggctg attttgatta tgccaccaga tttcaacagc tggatatgga
     1261 ggctctgaaa aaagatatca aagatttgct gacaacttcc caggattggt ggcctgcgga
     1321 ttatggtcat tatggtcctt tctttattcg tatggcttgg cacggtgccg gaacatacag
     1381 gacatatgat ggccggggag gcgccagtgg tggtcagcaa cgttttgaac cgctgaacag
     1441 ctggccggat aacgttaatc tggataaagc ccgtcgattg ctgtggccag tcaagaaaaa
     1501 atacggctcc agtatttcct ggggagacct gatggtcctg actggtaatg ttgcccttga
     1561 atccatggga tttaaaacgc tgggatttgc tggcggaaga gaagatgact gggagtcgga
     1621 cctggtatac tgggggcctg acaacaagcc tcttgcagat aaccgggata aaaacgggaa
     1681 acttcagaaa cctcttgccg ccacgcagat gggacttatt tatgtcaatc ctgaaggccc
     1741 cggtggaaaa ccagatcctc tggcttccgc gaaagatatc agggaagctt tttcacgtat
     1801 ggccatggat gatgaggaga ctgtggccct gatcgcggga gggcatacat ttggtaaagc
     1861 acatggtgca gcgtctcctg aaaaatgtat tggcgcaggg cctgatggtg cacctgtgga
     1921 ggagcaggga ctgggatgga aaaataaatg tggtacagga aacggcaaat ataccatcac
     1981 cagtggcctg gaaggagcct ggtcgacatc gccaacccag ttcacaatgc agtatctgaa
     2041 gaatttatat aaatatgaat gggagctgca caagagtcct gccggtgctt atcagtggaa
     2101 gcctaaaaaa gcggcaaata tagttcagga cgcgcatgat ccgtctgtcc tgcatccgtt
     2161 gatgatgttt acgacggata ttgctcttaa agttgatcct gaatataaga aaataaccac
     2221 ccgtttcctg aatgatccaa aagcttttga gcaggcattc gcaagagcat ggtttaaact
     2281 gacccaccgg gatatgggac cggcagcccg atatcttggt aatgaagttc ctgcagaatc
     2341 atttatctgg caggatcctc ttcctgcggc ggattataca atgattgatg gtaaagacat
     2401 taagtcgctg aaagagcagg ttatggattt gggtatccct gcatctgagc tgataaagac
     2461 agcctgggct tcagcttcca catttcgtgt gactgattat cgtgggggaa ataatggtgc
     2521 ccgcatcagg ttacagcccg aaattaactg ggaagttaat gagcctgaaa aactgaagaa
     2581 agtactggca tccctgacct cattacagcg tgaatttaac aaaaaacagt ctgacggaaa
     2641 gaaagtgtcg ttggctgatt taattgttct ttcgggtaat gctgcaatcg aagatgcggc
     2701 cagaaaagcc ggggtggaac ttgagattcc ctttactccg ggaagaactg acgcctctca
     2761 ggagcagacg gatgttgcct cattcagtgt actggagccg acagcagatg gattcagaaa
     2821 ttattactca aaaagcagaa gtcatatatc gccggttgaa agcctcattg ataaagccag
     2881 tcagctggat ctcaccgttc ctgaaatgac ggcattactg ggtggtctgc gggtaatgga
     2941 tattaataca aataattctt cgttgggagt gtttaccgat acccctggtg ttctggataa
     3001 caagtttttt gttaatctgc tggatatgtc aacacgatgg agtaaagcag ataaagaaga
     3061 tacatacaat ggattcgatc gtaaaacggg agcattaaaa tggaaagcat cctctgttga
     3121 tttaatcttc agttcaaatc ctgaattacg tgcggtggca gaagtatatg cctcggatga
     3181 tgcgagaaat aagtttattc atgattttgt taaatcgtgg aataaagtta tgaatagcga
     3241 tcggtttgat ttaaacaata aatgataaaa ctgatgaggg agggcacctc cctcatgaag
     3301 gagaaattat gcagaaaagc aataaagtta taatcagctg tggtatggca ttatattttt
     3361 tgtgtggggg gattacagtg gcagaagaac ttgatgaaag catgcaagtt cttgcaagga
     3421 atatgagtgt acttcagtca tcaatggata aaaaggagct tcttgaagct cttgacagaa
     3481 tggatgatgc tgtagatgag tcaatgaagc gtcttcctga gagcatttca ccggctgatg
     3541 aagaagggaa gaatgactat ataaaagaac ttcaaaagct tgaaaaagag attatgctgg
     3601 cgaaacaaaa agtcttatcg ggacagttat cagatatacc cgattcggta aacaagatga
     3661 ataaaatcag agttgagggg catgataaat tcagataaat cagttggttg attcctgcgg
     3721 gacgaataca tacgtcagga acataacttt atggcagtga accgccccgg gaatcctgga
     3781 gactaaactt cctgagaaag aggtaaacag gatgactaaa aatactcgtt tttcccccga
     3841 agtccgtcaa cgggcgattc atatggttct ggaaagtcag ggcgaatatg actcacaatg
     3901 ggcggcaatt tgttccattg cccaaagatt ggctgtacac cggagactct gcgtgtctgg
     3961 ggacgccagc atgagcggga taccgggagc ggtgatggtg ggctcaccag cgctgaacgt
     4021 cagcgtctga aagagctgga acgtgaaaac cgtgaactgc gccgcagtaa cgatatcctt
     4081 cgccaggctt ccgcttattt tgcgaaggcc gagttcgacc gcctctggaa aaaataatgc
     4141 cactgctgct gcgtgagcag tacggggtcg gaccggtatg cagtgaactg catattgccc
     4201 cgtcaacgta ttaccactgt cagcaacagc ggtatcatcc ttataaacgt agtgcctgtg
     4261 cacagcgcga tgactggctg aagaaagaga tactgcgctt atacgatgag aatcatcagg
     4321 tatacggtgg cgtaaagtct gacgtcagtt gttacaggaa ggtatcaggg tggccagatg
     4381 tatagtggcg cgcctcatgg cggttatggg acttgccggt gttctccggg gtaaaaaggt
     4441 ccgcactacc gtcagccgga aagctgttgc cgcaggcgac agcgtaaacc gccagttcgt
     4501 ggcagaacgt cctgaccagc tgtgggtggc ggattttact tacgtcagca catggcaggg
     4561 cttcgtctgt atggcgttcg atttagggca ctaaaaccgc tgctggtccc tcataataaa
     4621 agaagagcag gtaatagact taacctgctc ttcttttatt atttttttaa catagaaaga
     4681 ttgcgtctgg tttgttcttc gaaatcatct tttgaagtga tgctctctga tttgttttct
     4741 gaagatgccc ctatgatgtt ttttaacaca cttatatcaa ttgatgtaga tgtgtatcct
     4801 gttgtttgct ctataagttt caccagattt tctgcagtga ttttctcact gaaaaaacca
     4861 ctgattagcg ttaacagtcg ggggtcaaca gaataaagag ctgctccgca aataagagat
     4921 tgtctgtaga agtctccccg catttgtatc gggagagagt ctattgtttg ttgtgtcagg
     4981 ttatcctgcg gataaagtgt cgggtgcagg tatgccacaa ctttctttct ttttttcgga
     5041 tcactgcttt ccattacgca tgcaccattg ctttcaccaa tgcaaactga ggttcatcca
     5101 ccttgttcac tttcagattc gggaaatatt gctttatgtg agggtaaata agctcggctc
     5161 ctccgccagt gagatagatt cggttcattc ctgagaatgt tgaaatttcc tgcctgatgc
     5221 tttctgccag agatttcact tccctgtcta ttacatgagt aatgtttttt atttcagcta
     5281 catcatttat taatgtctgg agatattcat tatcatgtct gttttttatt aactcatcag
     5341 caataaaata attacatggt gtggaggctt tcgtcagcgc attcatgacg gtatgagtga
     5401 tgcgggatgt cccgatttct gaatacccac ggatttcaga aatgccttca aatgcaccga
     5461 gaattaaacc acaatccaga gtggttcctc caagatcgat aatcaggctg cgttcaagtt
     5521 tgtttacttt atcttttttt agtgattcaa atgcagctgg caaagattca ggcattacat
     5581 taactttttt aattttaaat gtttcccctt tattcagaga tatctcacgg agaacatttg
     5641 ccttttttct ttctattctt tcttcatttg gttgattgtc attatcaaag aattcggtta
     5701 caggtaatgt cactgtcagt tctacatcct gtggctgaag gccggatgtc agcagtgcat
     5761 gatgtatggc aagcaaattg gtggtgctgt attgataact gacatgtgtt gtacctatga
     5821 cagcagtact gcccaaatca taggtgtatt tttttccatc cacgacgtaa ttgtaaactt
     5881 tgccggcatt aaacagtccc gggttccagc cttctttaaa gctgtttcct gacagactgg
     5941 ttcttacctc gccatcttca agccaggcca gttttacgtt agttgaacca tcatcgcatg
     6001 atacttttag cattttttct tgcctcgttt tgagttttta tttatctcaa attgagcatc
     6061 ctttatctag atgcatcttt ttgagttttt tgttgttcct tattgtgtct cattttgggt
     6121 tcatgtcaag cgatgaggct cttttagagt ctctttgcat tcttgttgtg attcagtttg
     6181 agtctctatc gatttctcat tttgcgtttc gaactgagac ggaaactact taaccaggcc
     6241 gcatgtgaga ggagaattcc catgccaaac tggtgctcaa atcgtatgca tttttctggt
     6301 gaaccggcac agattgctga gattaaacga ctggccagcg gtgccgtcac accgctttat
     6361 cgccgcgcca caaatgaagg tattcagctg tttctggccg gaagtgccgg acttctgcag
     6421 accactgaag atgtgcggtt tgaaccatgc cccggactga cggctgccgg gcgtggcgtt
     6481 gtatcgccgg agaatatcgc gttcacccgc tggctgacat acctgcagaa cggtgtactg
     6541 ctggatgagc aaaactgcct gatgctgcat gaactctggc tgcagagcgg tactggacag
     6601 cgtcgctggg aaggattacc ggacgatgtc agggatacca tcaccgcact tttcaccgca
     6661 aaaagaggtg actggtgtgg cttctggagt aacgaggatg tatcggtgtg gtggaaccgt
     6721 ctgtgtgaca acgtactgcc ggaaaaaacc atgccgtttg acctgctgac ggttctgccg
     6781 acccgcctgg atgttgaagt gaatggcttt aacggtggtg ttctgaacgg tgttccttct
     6841 gcatatcact ggtatacgga acagtatggc gtgaagtggc ctgtggggta tgaggtgaat
     6901 atcagtagtc agggagacaa cttcattcag gttgatttcg acacgccgtg gtatcagccg
     6961 gaaagcgacg ttattgcaga attaagccgc cgtttcagct gcacactgga gcactggtat
     7021 gccgaacagg gctgcaattt ctgtggctgg cagcgatatg agcgtggcga gcttgttgat
     7081 gtgctgtggg gggagctgga atggtcacat ccgacagatg atgacgagtt gccggaagtc
     7141 accggacctg cgtggatagt cgacaaggtg gcgcattatg gcggatgaag tatgacggag
     7201 acgggcgggc aactgcccgt ttcttttccg gtaaaggatg tcgccgtgct cctcttttta
     7261 ctgcgccggc tgacgcggcg cggcataaac gctgtctgtg gtcagtgtcc cgcgtccggc
     7321 gggcacggga cgggcgcttt taccgctccc ggctggcgtc ggtgacagtg tacgccagcc
     7381 cgtcgccctt ttctgatgaa cgcccttcaa gccgctttcg cggcatagcc ttgccgtcaa
     7441 aaagacgaag gctgcggtat tccacggtcg gcctgacccg ttaccggacg cggtgaacag
     7501 cccacaggca gcgggtaacg ggcaccacag gggtgccctc ccggtgccct ctgtaagaga
     7561 aggagttttg tatgtcccgt tttgtcctcg gtaactgcat cgatgttatg gcccgtattc
     7621 ctgataacgc cattgatttc atactgactg acccgccgta cctcgtcggt ttccgtgacc
     7681 gttccgggcg caccattgcc ggtgacgtta atgatgactg gctgcaacct gccagcaatg
     7741 agatgtaccg cgtactcaaa aaagacgcgc tgatggtcag cttctacggc tggcaccgta
     7801 ttgaccgctt tatggcggtc tggatacgcg ccggattcag cgtggcaggg cacctcgtgt
     7861 tcaccaaaaa ctacacatcc aaatctgcgt atgtcgcgta ccgccacgaa tgcgcttaca
     7921 tcctggcaaa gggcagtccg gcccggcccc gtaacccgct gccggatgtg ctgggctgga
     7981 aatattcggg caaccgccat cacccgacgg aaaagcccgt taccagcctg caaccgctga
     8041 ttgagagctt cacacacccg aactcaattg tgctggaccc gtttgcaggc agcggttcaa
     8101 cctgcgtggc cgcccttcag tccggacgcc ggtatattgg tatcgagctg cttgaacagt
     8161 atcaccgtgc cggacagcaa cgccttgccg ccgtgcaacg ggccatgcag cagggggccg
     8221 caaatgataa caggtttgaa ccggaggcag catgatgaat tacagcggat ttgaagatat
     8281 gcgtgaacag gtggctgaac tggccaacac catgtgcgac ctgcgcacaa ccatgaatga
     8341 gatggagcag cggtacagcg atgcagacac cctgccggaa cgtctggtgc gtcagacgct
     8401 ctttcgtgcc agccacctgt ttatgaaggc atataacgaa atactggaac tggattactg
     8461 cttcaaagat tgaggggaaa gagtatgtac ggaacatgcg aaacgctctg ccgggcgctg
     8521 gcagcaaagt atccgggaga cacgccgttg atgctggtta tctggtcccc ggaagagatt
     8581 caggccctcg ctgacggaat ggatatttcc ctggccgatc atgaaatcag gaccatcctg
     8641 gcgcgcctgg aggacatccc ggaagaccag cggactgagt ccggtatttc ttcctccgcg
     8701 gcgatggaga ttatcagcaa cgtgagcgaa aaccgccagg tgaccgtccc tgctgaactg
     8761 ctggcgtccc tgattcagac cgctgaacag gcattgtgga agcgtgaatg ggccgcccgt
     8821 gattacggcc tcgccgtccc ggaatgcgtt acccgccgtc aggcggtggt taatcaggcc
     8881 cgcatcctgc tgaaaaacaa cacgcacgaa aacgactgat gttatcgccg cctccgggcg
     8941 gcgattcagg gagaccatga atcatgaata aaacgctgaa tgcactgatt tgtcgtcatg
     9001 cccgcgacct gctgctggcg cagggctggc cggaagagac ggatgttgac cagcgtaacc
     9061 tgaagtatcc gggctggatc agcatttacg tgcggctgga tgcgccccgg ctggcgacgt
     9121 tactcgttaa ccgccacgat ggcgtactgc cgccgcacct ggcctccgcc attcagaaac
     9181 tgaccggaac cggggcggaa cttggtttca cgccagccca cgaacgtttc cacctggcat
     9241 tatgttcgcc gggagacgtc tctcagctct ggatgctggt tctggtgaat tgtggtggac
     9301 agcctttcgc cgtcgttcag gtgcaacata tcttcacgcc tgtcgctatc agtcacacgc
     9361 tggcgcttgc cgcgacactt gatgcgcagg gatacagtgt taacgacatc atccatatcc
     9421 tgatggcaga aggaggtcag gcatgagcgc acgttcacgg gcactgattc ccctcagcgc
     9481 agaacaacag gccgccatgc aggctgtggc tgtcacagaa caacgtcgtc gtcagggacg
     9541 cacactttca gcatggcctt atgccagcgc tttctttcgc tgcctgaatg gcagtcgccg
     9601 gatttcgctg accgatctcc gcttttttgc ccctgcgctg acgaaggagg aatttcatgg
     9661 caaccgtctc ctgtggctgg ctgccgtgga taaactgatt gaaagttttg gtgaagtctg
     9721 tgttcttccc ctgccatccg atgcggggca tcgtctgttc ccgtccgttc cttttcgtga
     9781 aggtgagcgt cgtcgtcaga aaaccacgct gacagagcag aaatacagcc gccagcggga
     9841 acgtgaggca gaacgacggg aactggaata ccagacatgt tttgctcagg cgcagattga
     9901 ccttgcgttt catactcccg ccacggtcgg aagctggttg tcccgctggt ctggtgttgt
     9961 tgaggagcat gatctggaaa cgattttctg ggggtggtgc gggcgttttc catcactgtc
    10021 atcatttgac cggtttttct ggcaggagga accactctgg cggctgattt ttgaagccgg
    10081 tgaggccggt cgtggtgcac cggtacaggt acgtgcactt gagcagtgga tgatcccgaa
    10141 caagctggag aacgcaatat gatgaaatca gacgaacaat atcaggttcc cgtgtggatg
    10201 cgacctctgt tgccgttgct ctgcaatacc ggtggtaatg atccggagga acttctgaat
    10261 gatacagaaa ccaccgccag tgcgaatatt gtccgttatg tactgatagt tgcagtgcgg
    10321 tcgcagattg gatctgctgc agctactgta caggaaagga ctgttgcgca cagagatacc
    10381 aggtggcttt tcaccggaag aagcgcagga actcctggat aatctggtgc gcagccatat
    10441 cagcaaggcg ctgtcgggtg agcgaatggc agcccgtgac agatatgccg agctggcctg
    10501 gattcgccag caactggtgg atgccgcctg gtttgtccgt gccacactgg aagcgcatgg
    10561 tacgagcgtc ggaaatgaga gtccctctgc acttccagag acaatgccgg acatacagac
    10621 acgggaactg gtgatgctga tcaaacgact ggcatcttct ctgaaagcgg tgaaaccgga
    10681 cagtagcgtg gtgcgtgcgg cgcaggactg gctgtgcgac agaaaacttg tggatatcac
    10741 agatattctc cggtgaacca ggcatgtata tttttcgcag ggacacgccg atcatgatcg
    10801 tttcatatca ggggtggtct ggaggtcatg cggcgtgtcc tctgcactcg ccggaataag
    10861 gaagtcgccg gcggatccgc ttttacccgg ccatgcgggg catggccttg tgggttttca
    10921 gttatgtggc ctcagcgtcg tgtgcgggct gtgccgtgcc tccatcgtag ccgggcttct
    10981 gcgggcggga tgcaaggtaa gatgaaccgc tgcggtcacc cggtccctcc ttcccgtctg
    11041 ccgtgatttt ccggtcgttc acccgctcag atttcgcggt ctcaccctcc aactcccgtc
    11101 agccgtgcgc gtcgggctgc ggcttccctt gcgcccttgc atccctgcag gcccggcaaa
    11161 gatggaggca cggcaccgcc cggtgtcgca gaacatgtga ctatggagga ttcgggaatg
    11221 tctgttgttg cacctgctgt atacgttgga acctggcata aatacaactg tggaagcatc
    11281 gctggccgct ggtttgacct gaccacgttt gatgatgaac gcgacttttt cgccgcctgc
    11341 cgtgctcttc accaggatga agccgatcct gaactgatgt ttcaggatta tgaggggttc
    11401 ccggtgaata tggcctccga atgccatatc aactgggcct gggttgaagg cttccgccag
    11461 gcacgggatg aaggctgcga agaggcttat cgtctctggg tggatgatac cggtgagacg
    11521 gattttgaca ccttccgcga tgcctggtgg ggcgaggctg acagtgagga ggcttttgcg
    11581 gttgagttcg tcagtgatac cggcctgctg gctgacgtgc cggagacggt ggcgctctat
    11641 tttgactatg aggcgtatgc gcgggattta ttcctggact ccttcacctt tattgacggt
    11701 catgtgttcc gtcggtgatt tactcccccg ctccggcggg ggtatggcct gccgggccgc
    11761 cggaaaaccc aggcaggctt acgcctgcct gaaatacagg agggccgcgc ctgcggcgct
    11821 gcccttcctg cccgtcatgg tgtgaggcat ttttctgttt tgtcgtttct gttgttctga
    11881 tgcccggagg cggggacacc cttcggctcc cggctgcctg gcggcatccg gtcgtcggcc
    11941 cgactccgac aggcagctgc tggcgcaccc gccagtctcc gccgaacccg ctggcgcggt
    12001 ttcgggctgc cgctacggtc cgatgtctga aaaaccaggc ctgcagcgac cgttccggtc
    12061 gcgccgcccc tgtcgcgccg gaaacggcgc tccctgcaca cctgacggtg tgtaaggcca
    12121 ctgcgcctga tggcgcagtc aacccttctg ccgccacccc tgcggcctgt ttcccgctgt
    12181 ggactttccg ggggctgaag cggccacaac acggggcagc cgtccagggg aagatgtacc
    12241 agccacaaaa ctttttgccg caggcgtcaa aaacttttgc gtctgccccc ggacaactgc
    12301 cccgtgccgt ggcagtcgct gccccatcgg aaaggtccac tgcggggaga aaccccgaag
    12361 aagtggacgg cagaaggggc tttcgcccct ccgggggagc aaccggtcgg cccggcgacc
    12421 tgattcagca gaaggagacg ttttcatgac agtcagcagc acgatttccg ttttttgccg
    12481 cgacggggtg ttccgtaccg tttactgcca cctgcacggg gagccgacct ggaacggtcg
    12541 catcctgcat acccactatg ccaccggtca gctggccgaa gccctggttg aacacggaga
    12601 tatccgttgc ctcggtcccc gttgcgacaa atccgccggg catacgcttc agaacccggt
    12661 ggacggtgtg acggcttatt acggacgtga cagtggtttc cggatggaca gtgaggcgcg
    12721 tgagtaccgt tctttcatgg aggctattgc cactgaaagc actgaagagg tgcgcttcca
    12781 ttatgtgttc atcgacggct actggaaggt gatgtaccgc acgccggaag gctggaagat
    12841 gaaagcgctc gcgctggcac tgcgccgctg tccgaaatga aaaaaggcag ggcgataaac
    12901 cctgcctttc tccgccggcg cttccccgta cggaagtccg gcatctcaat cactatctat
    12961 ggagattatg ccatgagacc atccattatc ttcgcaaccg ccgagtatgt aaagcgtctg
    13021 cgtgaagagt gcctgcggga gaataaaccc ctgcaccgcc atacccgcgt cagacgtcag
    13081 gagctggcac aggatgagat taacccggac gtcctggcga tgagcggtca tatcgccaga
    13141 cgctgcagtg agcagaagcg ggtacgtatc cccgccatga aagtcagcga atggggccac
    13201 ctgctccgcg cgcttgaaat tgagcgggcc tgccactgat taacccttgc cgtctgcctg
    13261 tgcaggcggc aggatacgtt cagacatctc accaggagga accgtgagta agaagaaaaa
    13321 caccacaacg cccacgccgc atgatgccgc gttctggtcg ttcctggcga atcccgacgt
    13381 cgccagagat tttctggaac tgcatcttcc ggcggagtac cggcagttgt gcgacctgtc
    13441 cacgctgaag ctggaacccg ccacctttgt tgagccggac ctgcatcagt acgccagcga
    13501 tatcctctgg agtgtgaaaa ccaccggggg tgaagacggc tatgtttata cgctcatcga
    13561 gcaccagagc accgaaaatc tgtacatgcc tttccgcatg ttacgttaca gtgtggcggc
    13621 gatgcagaga catctggagc agcacaaaac attgccactg gtgattccgg tgctgttcta
    13681 tcatggtgag cgcagcccgt acccgtacag catgaactgg ctggactgtt ttgaggaacc
    13741 ggcgcttgcg gctaaaatat acacaaagcc gtttccgctg gttgatatca ctgtcgttga
    13801 tgacaatgaa atcatgaacc atcgccggat ggccgcactg acgctgctga tgaagcatat
    13861 ccgccatcgt gacatgatgg agctgctgga caaactcccg caggtcatgg tggaaatttc
    13921 agacgagcag gtgcgtgttc tgattcatta catcgttaac gcaggggact ctgtatcacc
    13981 ggaatttatg cgggcgctgg ctgagcgtct gccgcagcat gaggataaac tgatgactat
    14041 cgctgaacgt cttgagcaaa aaggtcgcca ggaaggagcg cttgaaaaag ccctggctat
    14101 tgcgtgccag cttcagaaaa tggggatgac gccggagcag attaagcagg ctaccggact
    14161 ttccgaagct gaactgaaga acatcactca ctgatgagaa ccacaaagcc cggcaaaatg
    14221 ccgggttttt ttgtcagcag gagcagagca aacagcatga cgattgcaga acgtcttata
    14281 cagaaaggtt tcgacgaagg ttttaagaaa ggtgcgcttg aagtggcgcg ggaagcagcc
    14341 tgccggctgc gggatatggg ctggacgccg gaacggattc aggaggcaac cggactttcc
    14401 ggtgaagaac tgaaaaagct gtttcctgat gagcagtagc ctggcattct gccaggctgt
    14461 gaatcacgct cacacattcc ctgccgtttc ccccagttca ttttccagac gcctgatgat
    14521 atctgccttg cgcatcccct gcatgtgagg cagcagatca cggactgaaa gtgcctgggt
    14581 ggtcctccag acggtgacat ccacctgttc cgcattttcc caggcgtgcc atgttctctg
    14641 tgtcacgcca aattctctgg ccgctttttc gcgtgaccag agcatgcttt ttcgccagat
    14701 tcgcagttcc cagccggtca taaaacacct ttgaaaaagt gaaaaaactt cactttattt
    14761 tatcatcata atcaccaggt cagcgcgata aataagagtg aaaaagtgaa gaaatttcac
    14821 tttttagttg taggtgtcgt gctgttttct ctgttttcac ctgtgcggga gtggtgcgcc
    14881 agtgaccacc acatgccatt gttgtgctgt cgtttttctt cagtcgcgcc ggatgcggcg
    14941 ctccctgcac acctgacggt gtgtaaggcc actgcgcctg atggcgcagc tgtctctgcc
    15001 tgtctgccgc cgcactgcgg cccgttctcc gcagacggac tttccggggg tacatacacg
    15061 accacatccc gtttcagtcg tccaggggag agatgtacca gccacaaaac tttttgccgc
    15121 aggcgtcaaa aacttttgcg tctgcccccg gacaactgcc ccgtgacgtg gtgagcgtcc
    15181 cccatcggaa aggtccactg cgggaagaac cccctgtgaa gtggacggca gaaggagctt
    15241 cgctcctccg ggaaatgacc aggctgaccc ggcagccgtg acgatgagga gacaatatga
    15301 tgaatcagac tttacccact gctgacctga atactgccgg cacgacagat attattccgt
    15361 ctatggctat cgaccgcatc atcgcgcagc gtaacgaagg tattgcactg ttcatgcagg
    15421 cgatggaatg cctggcgaca gcgcgcagga ttctgctcga tgcttccggt gacatttttc
    15481 tctacgggtt tgaagactgc gtgactgact ccgttcgatg catggataaa ccggaagaag
    15541 caaaaaagaa tatcacccgt cttgccgacc ggaaaatctg ggatcgcctg atgacttata
    15601 cgggcatgta caccttcatg agttcatgcc agcgtgatga gtggaacagc cagctgatga
    15661 gcgacacctg tcctgaaatc accctggaca atgtactggc aacattccgc catctgaatg
    15721 ccagtaagat gcagacattt gaacaggaac tgattgatat ctggcggaaa ttgtcatggg
    15781 attacagaac caataatccc tgccgtctgg gcaagagaat cattattgaa aacctgctgt
    15841 accgctggag taacgggcgt gtgacgctgg actgcagcgg acgggaggca ctggatgacc
    15901 tggtacgtcc gttttatctg ctggagggac gcaacgttcc tgacttcagg aacagcatcg
    15961 gggcgcagta tggtgaattt ctcgggaacg gcgacaatat cggtgagttg tttgaagggg
    16021 aatattttac ggtgcgtggc taccagaaag ggaccgtaca cattgtcttt aagcgtcctg
    16081 accttgttga aaaactgaat gatattattg cacggcatta tcctggtgta ttgccgccac
    16141 gagtctgatt aaacagaaaa gccctggtat ttatgccggg gctttttttt ctgtaatcat
    16201 cataatctca gaatacgatg tgacattgtc acatcaattg ttgttcatat cgatgccatc
    16261 tttcctgcct gaatctgtct ttttaggtgc tgttttagag gaagaacggc gggatggttt
    16321 tttcgattct gcttcccgaa taatgtcacg aagtgccttc tctcctactt cataccctgc
    16381 atttttgagc gtgtcacgaa catctgccag tgtataaccc tttgtcctta ccaggacaat
    16441 gatgtcatca cgaatggcat caagaaaatc acgcaatgtt tttcgttgag cggtgaggtc
    16501 cggcaattct gataaagcag cttttgccag ctggatgtca tcatctgagt aaaatttttt
    16561 agaagccatt ggcacttttt ctccgtttca tgttgaagtc tgattttatc atcaaaaact
    16621 gcctcctggc agagctgcgc cccgcctctg ccgtctgcat aagactatga tgcacaaaaa
    16681 taacaggtta taatgcctga aaaacgggac aggatgtgta atcgtataac cgcgcacgca
    16741 cggctatacg attaccacct ggtcagggct tagccccgac accccggtaa ggagcagacc
    16801 atgagtgaga agaaaacccg aagtggcagt gagaaacggc aaaagaatgt cctcattgct
    16861 gttcgttttt caccagaaga ggctgagatt gttaaagaaa aggcagaaaa aaacggactg
    16921 acagtttcaa ctctgataag aaaaactgtg ctgggaaaac agataaatgc ccgcattgac
    16981 gaagattttt tgaaagagct gatgcgtctt ggtcgcctgc agaaacatct gtttgtggaa
    17041 ggaaaacgca cgggagataa agagtatgca gaggttctcg ttgctattac agagcttgct
    17101 aatactctgc gaagagattt aatgggacgc tgagataatc gctgatgaat gccatcattc
    17161 cgaaaaaaag acgcgacgga aagtcttctt ttgaagacct gattgcgtac acctcagtgc
    17221 gggatgatgt gccggaagat gaactcaggc cagacacgga aatgaaagct gagattccgc
    17281 accggaaccg cttcagacgt cttgttgatt atgcaacacg tctgcgtaat gaaaaatttg
    17341 tttccctgat tgatgtgatg aaagacggca gtcagtgggt gaatttctat ggtgtgacct
    17401 gctttcataa ctgtttgtct cttgaatctg cggctgaaga aatgcagaaa gcagcagacc
    17461 ttgctcattt ttccaaagat gatacagatc ctgtttttca ctatatactc tcctggcctg
    17521 cgcacgaaag tccgcgttct gagcagcttt ttgactgtgt gcgtcacacc ctgaaatccc
    17581 ttgagctgtc aaaacatcag tatgtggcag ctgttcatac agacacggat aacctgcatg
    17641 tacatgttgc agtgaatcgt gtccaccctg aaaccggcta tatcaattgc cttccgtgga
    17701 gccaggaaaa actcagtcgt gcatgccgtg aacttgaaat taagcacgga ttcgcgccgg
    17761 ataatggctg ctttgtgcat gcaccgggca atcgtattgt gcgcaaaaca gcgctggtac
    17821 gtgagcgccg taatgcctgg cgacgcggga aaaagcagac gttcagggaa tatattgttc
    17881 agatgtcgat tgccgggctg cgtgaagagc ccgcacagga ctggttatct ttgcataaac
    17941 ggctggccag tgatggtctg tatatcacga tgcaggaagg ggaactggtg gtgaaggatg
    18001 gctgggacag agcgcgggaa ggtgttgcac tcagttcgtt cggaccgtta tggacagctg
    18061 aaaaactggg gcggaaactg ggggaatatc agcctgtacc gacagatatt ttcagtcagg
    18121 tggggacacc cggtcgttat gatccggaag ccataaacgt tgatatccgg ccggaaaaag
    18181 tggcagaaac ggagagtctg aaacagtacg cctgccgtca ttttgccgaa cgcttaccgg
    18241 aaatggcgag aaatggtgag ctggaaagtt gcctggatgt gcacagaact ctggcaacgg
    18301 ctggattatg gatggggatc cagcacgggc atctggtact gcatgatgga tttgataaac
    18361 aacagacacc agtacgtgca gacagtgtgt ggccgttaat gacgctggat tatatgcagg
    18421 atctggatgg tggatggcag cctgtaccga aggatatttt tactcaggtt atacccggag
    18481 aacggttccg gggacgtaat cttggtactc aggctgtcag tgattacgaa tggtatcgta
    18541 tgcggatggg gacaggtcca cagggagcaa taaagcgcga actgttttct gataaggaaa
    18601 gtctgtgggg atacggcctt attcagtgca ggagcctgat tgaagagatg atagctgacg
    18661 gggatttcag ctggcaggca tgtcatgaga tgtttgcgcg taaagggctt atgctgcaga
    18721 aacagcatca tggtcttgtg attgtggatg cgtttaatca tgagctgaca ccggtgaagg
    18781 ccagcagtat ccatcctgat ttgacgttat cccgtgccga gcctcaggcc gggccatttg
    18841 aaattgcggc tgcagatatt ttcgaacggg tgaagcctga atgtcgttac aatccggaac
    18901 ttgctgccag tgatgaagtg gaaccaggct tccggcgcga tcctgagatt cgtcgtgagc
    18961 gccgtgaagc ccgtgcagct gcgcgggaag atttgcgtgc gcgttatctt gcatggaagg
    19021 agcactggcg taaacccgac ttacgttacg gagaacggtt acgggaaatt catgctgcct
    19081 gccgtcggcg caaggcgtat atccgtgtgc agttccggga tccacaattg cgtaagctgc
    19141 attaccatat tgcggaagtg cagcgtatgc aggcgctgat caggctgaag gagagtgtga
    19201 aggaagagcg gttgtcactg attgcggagg gtaagtggta tcctttgtcc taccggcagt
    19261 gggtggaaca acaggcggct cagggagaca gggctgcagt atcgcagtta cgtggctggg
    19321 attatcgtga tcgtcgctcg cgtaataagg ataaaagacg cacaactaat gttgatcgtt
    19381 gtgtcgtact ctgcgaaccc ggaggaacgc cactttttaa taatgttgca aaacttgaag
    19441 ctcgtcttca gaaaaatgga agtgttcatt tcagggatac gcggacagga aaaaatgtct
    19501 gtacggatta cggagatcgg gttgtgttct accatcacac ggaccgaaat gaactggcag
    19561 agaagcttga tttaattgcg ccagttctgt tcagccgcaa cggaaagctt ggttttgagc
    19621 cggaaggttc ataccagcag tttaatgatg tgtttgcaga aatggtggcc tggcacaatg
    19681 cagcgggaat aaccggaaac ggacacttca ccattactcg tccggacgtg gatttgcacc
    19741 gtcaacgcag tgagcagtat taccgggaat atatcagaca gcagacccgt ttatcggtga
    19801 gccatgatga taattatacg ttacggcagg agaaaacatg ggagccacca tcacctggaa
    19861 tgtaagcccg gataagtgat ggtgcaaaga ttcagcccaa aaacggcagg aaccttcgtc
    19921 aataagggcg ggagtagtca ctgaattgag tttatacccg gttaactctg aattaagggc
    19981 cgtgtctgcg gcccttatcc agtgcgccgt aaaaccccgg acttagtgaa cagggtttta
    20041 cagtctgatt gacagatatg gtcaccagaa aatatttgta ttccttcagt atttttcgtc
    20101 agttgttggt tgtgctccac atgatttctg gggatgatca ttatttattg tctgcgattt
    20161 attattttca gcgaccggta acagtatctc cagggggagc agggtattca gaatcgcctt
    20221 cactgaaggt atatcttcag gatcagagtg ttcctcttcc ggacgattca gtgtgatata
    20281 acatacccgg cgttcttccc tggttgtgtt attgatagtg tgcacagcct ttttccacag
    20341 ttcgtttgcc cgggattcga tatctgtatc atattgcg
//
LOCUS       DAZKHF020000066        19962 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2201S31241
            SAMN43267800-rid25106413.denovo.066, whole genome shotgun sequence.
ACCESSION   DAZKHF020000066 DAZKHF020000000
VERSION     DAZKHF020000066.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267800
            Sequence Read Archive: SRR30307263
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 19962)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19962)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 19962)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350581.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350581.2
            Genome Coverage       :: 151x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:21:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,652
            CDSs (total)                      :: 5,557
            Genes (coding)                    :: 5,320
            CDSs (with protein)               :: 5,320
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 237
            CDSs (without protein)            :: 237
            Pseudo Genes (ambiguous residues) :: 0 of 237
            Pseudo Genes (frameshifted)       :: 102 of 237
            Pseudo Genes (incomplete)         :: 130 of 237
            Pseudo Genes (internal stop)      :: 52 of 237
            Pseudo Genes (multiple problems)  :: 41 of 237
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19962
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267800-rid25106413.denovo.066"
                     /serotype="O 26:H11"
                     /isolate="2201S31241"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-01"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            complement(138..884)
                     /gene="cutC"
                     /locus_tag="ACHOSC_RS09760"
                     /old_locus_tag="pgaptmp_001952"
     CDS             complement(138..884)
                     /gene="cutC"
                     /locus_tag="ACHOSC_RS09760"
                     /old_locus_tag="pgaptmp_001952"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_025309.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005507 - copper ion binding [Evidence
                     IEA];
                     GO_process: GO:0055070 - copper ion homeostasis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="copper homeostasis protein CutC"
                     /protein_id="HGU4547894.1"
                     /translation="MALLEICCYSMECALTAQQNGADRVELCAAPKEGGLTPSLGVLK
                     SVRQRVTIPVHPIIRPRGGDFCYSDGEFAAILEDVRTVRELGFPGLVTGVLDVDGNVD
                     MPRMEKIMAAAGPLAVTFHRAFDMCANPLYTLNNLAELGIARVLTSGQKSDALQGLSK
                     IMELIAHRDAPIIMAGAGVRAENLHHFLDAGVLEVHSSAGAWQASPMRYRNQGLSMSS
                     DEHADEYSRYIVDGAAVAEMKGIIERHQAK"
     gene            complement(898..1464)
                     /gene="yecM"
                     /locus_tag="ACHOSC_RS09765"
                     /old_locus_tag="pgaptmp_001953"
     CDS             complement(898..1464)
                     /gene="yecM"
                     /locus_tag="ACHOSC_RS09765"
                     /old_locus_tag="pgaptmp_001953"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310612.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HGU4547895.1"
                     /translation="MANWQSIDELQDIASDLPRFTHALDELSLRLGLNITPLTADHIS
                     LRCHQNATAERWRRGFEQCGELLSENMINGRPICLFKLHEPVQVAHWQFSIVELPWPG
                     EKRYPHEGWEHIEIVLPGDPETLNARALALLSDEGLSLPGISVKTSSPKGEHERLPNP
                     TLAVTDGKTTIKFHPWSIEEIVASEQSA"
     gene            1680..3413
                     /gene="argS"
                     /locus_tag="ACHOSC_RS09770"
                     /old_locus_tag="pgaptmp_001954"
     CDS             1680..3413
                     /gene="argS"
                     /locus_tag="ACHOSC_RS09770"
                     /old_locus_tag="pgaptmp_001954"
                     /EC_number="6.1.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001025321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004814 - arginine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006420 - arginyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine--tRNA ligase"
                     /protein_id="HGU4547896.1"
                     /translation="MNIQALLSEKVRQAMIAAGAPADCEPQVRQSAKVQFGDYQANGM
                     MAVAKKLGMAPRQLAEQVLTHLDLNGIASKVEIAGPGFINIFLDPAFLAEHVQQALAS
                     DRLGVSTPEKQTIVVDYSAPNVAKEMHVGHLRSTIIGDAAVRTLEFLGHKVIRANHVG
                     DWGTQFGMLIAWLEKQQQENAGEMELADLEGFYRDAKKHYDEDEEFAERARNYVVKLQ
                     SGDEYFREMWRKLVDITMTQNQITYDRLNVTLTRDDVMGESLYNPMLPGIVADLKAKG
                     LAVESEGATVVFLDEFKNKEGEPMGVIIQKKDGGYLYTTTDIACAKYRYESLHADRVL
                     YYIDSRQHQHLMQAWAIVRKAGYVPESVPLEHHMFGMMLGKDGKPFKTRAGGTVKLAD
                     LLDEALERARRLVAEKNPDMPADELEKLANAVGIGAVKYADLSKNRTTDYIFDWDNML
                     AFEGNTAPYMQYAYTRVLSVFRKAEINEEQLAAAPVIIREDREAQLAARLLQFEETLT
                     VVAREGTPHVMCAYLYDLAGLFSGFYEHCPILSAENEEVRNSRLKLAQLTAKTLKLGL
                     DTLGIETVERM"
     gene            complement(3455..3499)
                     /gene="yecV"
                     /locus_tag="ACHOSC_RS09775"
                     /old_locus_tag="pgaptmp_001955"
     CDS             complement(3455..3499)
                     /gene="yecV"
                     /locus_tag="ACHOSC_RS09775"
                     /old_locus_tag="pgaptmp_001955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YecV"
                     /protein_id="HGU4547897.1"
                     /translation="MSIFRIHLDGNKKA"
     gene            3590..4078
                     /gene="yecT"
                     /locus_tag="ACHOSC_RS09780"
                     /old_locus_tag="pgaptmp_001956"
     CDS             3590..4078
                     /gene="yecT"
                     /locus_tag="ACHOSC_RS09780"
                     /old_locus_tag="pgaptmp_001956"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416391.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysozyme inhibitor LprI family protein"
                     /protein_id="HGU4547898.1"
                     /translation="MFKFLVLTLGIISCQAYAEDTVIVNDHDISAIKDCWQKNSDDDT
                     DINVIKSCLRQEYNLVDAQLNKAYGEAYRYIEQVPRTGVKKPDTEQLNLLKKSQRAWL
                     DFRDKECELILSNEDVQDLSDPYSESEWLSCMIIQTNTRTRQLQLYRNSEDFYPSPLT
                     RG"
     gene            complement(4198..4590)
                     /gene="flhe"
                     /locus_tag="ACHOSC_RS09785"
                     /old_locus_tag="pgaptmp_001957"
     CDS             complement(4198..4590)
                     /gene="flhe"
                     /locus_tag="ACHOSC_RS09785"
                     /old_locus_tag="pgaptmp_001957"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar protein FlhE"
                     /protein_id="HGU4547899.1"
                     /translation="MRTLLAILLFPLLVQAAGEGMWQASSVGITLNHRGESMSSAPLS
                     TRQPASGLMTLVAWRYQLIGPTPSGLRVRLCSQSRCVELEGQSGTTVAFSGIAAAEPL
                     RFIWEVPGGGRLIPPLKVQRNEVIVNYR"
     gene            complement(4590..6668)
                     /gene="flhA"
                     /locus_tag="ACHOSC_RS09790"
                     /old_locus_tag="pgaptmp_001958"
     CDS             complement(4590..6668)
                     /gene="flhA"
                     /locus_tag="ACHOSC_RS09790"
                     /old_locus_tag="pgaptmp_001958"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0044780 - bacterial-type flagellum assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis protein FlhA"
                     /protein_id="HGU4547900.1"
                     /translation="MSNLAAMLRLPANLKSTQWQILAGPILILLILSMMVLPLPAFIL
                     DLLFTFNIALSIMVLLVAMFTQRTLEFAAFPTILLFTTLLRLALNVASTRIILMEGHT
                     GAAAAGKVVEAFGHFLVGGNFAIGIVVFVILVIINFMVITKGAGRIAEVGARFVLDGM
                     PGKQMAIDADLNAGLIGEDEAKKRRSEVTQEADFYGSMDGASKFVRGDAIAGILIMVI
                     NVVGGLLVGVLQHGMSMGHAAESYTLLTIGDGLVAQIPALVISTAAGVIVTRVSTDQD
                     VGEQMVNQLFSNPSVMLLSAAVLGLLGLVPGMPNLVFLLFTAGLLGLAWWIRGREQKA
                     PVEPKPVKMAENNTVVEATWNDVQLEDSLGMEVGYRLIPMVDFQQDGELLGRIRSIRK
                     KFAQEMGFLPPVVHIRDNMELQPARYRILMKGVEIGSGDAYPGRWLAINPGTAAGTLP
                     GEATVDPAFGLNAIWIESALKEQAQIQGYTVVEASTVVATHLNHLISQHAAELFGRQE
                     AQQLLDRVAQEMPKLTEDLVPGVVTLTTLHKVLQNLLDEKVPIRDMRTILETLAEHAP
                     IQSDPHELTAVVRVALGRAITQQWFPGKDEVHVIGLDTPLERLLLQALQGGGGLEPGL
                     ADRLLAQTQEALSRQEMLGAPPVLLVNHAPRPLLSRFLRRSLPQLVVLSNLELSDNRH
                     IRMTATIGGK"
     gene            complement(6661..7809)
                     /gene="flhB"
                     /locus_tag="ACHOSC_RS09795"
                     /old_locus_tag="pgaptmp_001959"
     CDS             complement(6661..7809)
                     /gene="flhB"
                     /locus_tag="ACHOSC_RS09795"
                     /old_locus_tag="pgaptmp_001959"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0015031 - protein transport [Evidence IEA];
                     GO_process: GO:0044780 - bacterial-type flagellum assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis protein FlhB"
                     /protein_id="HGU4547901.1"
                     /translation="MSDESDDKTEAPTPHRLEKAREEGQIPRSRELTSLLILLVGVSV
                     IWFGGVSLARRLSGMLSAGLHFDHSIINDPNLILGQIILLIREAMLALLPLISGVVLV
                     ALISPVMLGGLVFSGKSLQPKFSKLNPLPGIKRMFSAQTGAELLKAILKTILVGSVTG
                     FFLWHHWPQMMRLMAESPITAMGNAMDLVGLCALLVVLGVIPMVGFDVFFQIFSHLKK
                     LRMSRQDIRDEFKQSEGDPHVKGRIRQMQRAAARRRMMADVPKADVIVNNPTHYSVAL
                     QYDENKMSAPKVVAKGAGLVALRIREIGAENNVPTLEAPPLARALYRHAEIGQQIPGQ
                     LYAAVAEVLAWVWQLKRWRLAGGQRPVQPTHLPVPEALDFINEKPTHE"
     gene            complement(8011..8655)
                     /gene="cheZ"
                     /locus_tag="ACHOSC_RS09800"
                     /old_locus_tag="pgaptmp_001960"
     CDS             complement(8011..8655)
                     /gene="cheZ"
                     /locus_tag="ACHOSC_RS09800"
                     /old_locus_tag="pgaptmp_001960"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0050920 - regulation of chemotaxis
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein phosphatase CheZ"
                     /protein_id="HGU4547902.1"
                     /translation="MMQPSIKPADEHSAGDIIARIGSLTRMLRDSLRELGLDQAIAEA
                     AEAIPDARDRLYYVVQMTAQAAERALNSVEASQPHQDQMEKSAKALTQRWDDWFADPI
                     DLADARELVTDTRQFLADVPAHTSFTNAQLLEIMMAQDFQDLTGQVIKRMMDVIQEIE
                     RQLLMVLLENIPEQESRPKRENQSLLNGPQVDTSKAGVVASQDQVDDLLDSLGF"
     gene            complement(8666..9055)
                     /gene="cheY"
                     /locus_tag="ACHOSC_RS09805"
                     /old_locus_tag="pgaptmp_001961"
     CDS             complement(8666..9055)
                     /gene="cheY"
                     /locus_tag="ACHOSC_RS09805"
                     /old_locus_tag="pgaptmp_001961"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002210873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis response regulator CheY"
                     /protein_id="HGU4547903.1"
                     /translation="MADKELKFLVVDDFSTMRRIVRNLLKELGFNNVEEAEDGVDALN
                     KLQAGGYGFVISDWNMPNMDGLELLKTIRADGAMSALPVLMVTAEAKKENIIAAAQAG
                     ASGYVVKPFTAATLEEKLNKIFEKLGM"
     gene            complement(9070..10119)
                     /gene="cheB"
                     /locus_tag="ACHOSC_RS09810"
                     /old_locus_tag="pgaptmp_001962"
     CDS             complement(9070..10119)
                     /gene="cheB"
                     /locus_tag="ACHOSC_RS09810"
                     /old_locus_tag="pgaptmp_001962"
                     /EC_number="3.1.1.61"
                     /EC_number="3.5.1.44"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000036372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-glutamate methylesterase/protein
                     glutamine deamidase"
                     /protein_id="HGU4547904.1"
                     /translation="MSKIRVLSVDDSALMRQIMTEIINSHSDMEMVATAPDPLVARDL
                     IKKFNPDVLTLDVEMPRMDGLDFLEKLMRLRPMPVVMVSSLTGKGSEVTLRALELGAI
                     DFVTKPQLGIREGMLAYSEMIAEKVRTAAKASLAAHKPLSAPTTLKAGPLLSSEKLIA
                     IGASTGGTEAIRHVLQPLPLSSPALLITQHMPPGFTRSFADRLNKLCQIGVKEAEDGE
                     RVLPGHAYIAPGDRHMELSRSGANYQIKIHDGPAVNRHRPSVDVLFHSVAKQAGRNAV
                     GVILTGMGNDGAAGMLAMRQAGAWTLAQNEASCVVFGMPREAINMGGVCEVVDLSQVS
                     QQMLAKISAGQAIRI"
     gene            complement(10122..10982)
                     /gene="cheR"
                     /locus_tag="ACHOSC_RS09815"
                     /old_locus_tag="pgaptmp_001963"
     CDS             complement(10122..10982)
                     /gene="cheR"
                     /locus_tag="ACHOSC_RS09815"
                     /old_locus_tag="pgaptmp_001963"
                     /EC_number="2.1.1.80"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein-glutamate O-methyltransferase CheR"
                     /protein_id="HGU4547905.1"
                     /translation="MTSSLPCGQTSLLLQMTERLALSDAHFRRISQLIYQRAGIVLAD
                     HKRDMVYNRLVRRLRSLGLTDFGHYLNLLESNQHSGEWQAFINSLTTNLTAFFREAHH
                     FPLLADHARRRSGEYRVWSAAASTGEEPYSIAMTLADTLGTAPGRWKVFASDIDTEVL
                     EKARSGIYRHEELKNLTPQQLQRYFMRGTGPHEGLVRVRQELANYVDFAPLNLLAKQY
                     TVPGPFDAIFCRNVMIYFDQNTQQEILRRFVPLLKPDGLLFAGHSENFSHLERRFTLR
                     GQTVYALSKD"
     gene            complement(11001..12605)
                     /gene="tap"
                     /locus_tag="ACHOSC_RS09820"
                     /old_locus_tag="pgaptmp_001964"
     CDS             complement(11001..12605)
                     /gene="tap"
                     /locus_tag="ACHOSC_RS09820"
                     /old_locus_tag="pgaptmp_001964"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein IV"
                     /protein_id="HGU4547906.1"
                     /translation="MFNRIRISTTLFLILILCGILQIGSNGMSFWAFRDDLQRLNQVE
                     QSNQQRAALAQTRAVMLQASTALNKAGTLTALSYPADDIKTLMTTARASLTQSTTLFK
                     SFMAMTAGNEHVRALQKETEKSFARWHNNDLEHQATWLESNQLSDFLTAPVQESQNAF
                     DVNFEAWQLEINHVLEAASAQSQRNYQISALVFISMIIVAAIYISSALWWTRKMIVQP
                     LAIIGSHFDSIAAGNLARPIAVYGRNEITAIFASLKTMQQALRGTVSDVRKGSQEMHI
                     GIAEIVAGNNDLSSRTEQQAASLAQTAASMEQLTATVGQNADNARQASELAKNAATTA
                     QAGGVQVSTMTHTMQEIATSSQKIGDIISVIDGIAFQTNILALNAAVEAARAGEQGRG
                     FAVVAGEVRNLASRSAQAAKEIKGLIEESVNRVQQGSKLVNNAAATMIDIVSSVTRVD
                     DIMGEIASASEEQQRGIEQVAQAVSQMDQVTQQNASLVEEAAVATEQLANQADHLSSR
                     VAVFTLEEHEVARHESAQLQIAPVVS"
     gene            complement(12651..14312)
                     /gene="tar"
                     /locus_tag="ACHOSC_RS09825"
                     /old_locus_tag="pgaptmp_001965"
     CDS             complement(12651..14312)
                     /gene="tar"
                     /locus_tag="ACHOSC_RS09825"
                     /old_locus_tag="pgaptmp_001965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004888 - transmembrane signaling receptor
                     activity [Evidence IEA];
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein II"
                     /protein_id="HGU4547907.1"
                     /translation="MINRIRVVTLLVMVLGVFALLQLISGSLFFSSLHHSQKSFVVSN
                     QLREQQGELTSTWDLMLQTRINLSRSAVRMMMDSSNQQSNAKVELLDSARKTLAQAAT
                     HYKKFKSMAPLPEMVATSRNIDEKYKNYHTALTELIDYLDYGNTGAYFAQPTQGMQNA
                     MGEAFAQYALSSEKLYRDIVTDNADDYRFAQWQLAVIALVVVLILLVAWYGIRRMLLT
                     PLAKIIAHIREIAGGNLANTLTIDGRSEMGDLAQSVSHMQRSLTDTVTHVREGSDAIY
                     AGTREIAAGNTDLSSRTEQQASALEETAASMEQLTATVKQNADNARQASQLAQSASDT
                     AQHGGKVVDGVVKTMHEIADSSKKIADIISVIDGIAFQTNILALNAAVEAARAGEQGR
                     GFAVVAGEVRNLASRSAQAAKEIKALIEDSVSRVDTGSVLVESAGETMNNIVNAVTRV
                     TDIMGEIASASDEQSRGIDQVALAVSEMDRVTQQNASLVQESAAAAAALEEQASRLTQ
                     AVSAFRLAASPLTNKPQTPSRPASEQPPAQPRLRIAEQAPNWETF"
     gene            complement(14457..14960)
                     /gene="cheW"
                     /locus_tag="ACHOSC_RS09830"
                     /old_locus_tag="pgaptmp_001966"
     CDS             complement(14457..14960)
                     /gene="cheW"
                     /locus_tag="ACHOSC_RS09830"
                     /old_locus_tag="pgaptmp_001966"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheW"
                     /protein_id="HGU4547908.1"
                     /translation="MTGMTNVTKLASEPSGQEFLVFTLGDEEYGIDILKVQEIRGYDQ
                     VTRIANTPAFIKGVTNLRGVIVPIVDLRIKFSQVDVDYNDNTVVIVLNLGQRVVGIVV
                     DGVSDVLSLTAEQIRPAPEFAVTLSTEYLTGLGALGDRMLILVNIEKLLNSEEMALLD
                     SAASEVA"
     gene            complement(14981..16945)
                     /gene="cheA"
                     /locus_tag="ACHOSC_RS09835"
                     /old_locus_tag="pgaptmp_001967"
     CDS             complement(14981..16945)
                     /gene="cheA"
                     /locus_tag="ACHOSC_RS09835"
                     /old_locus_tag="pgaptmp_001967"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010283911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheA"
                     /protein_id="HGU4547909.1"
                     /translation="MSMDISDFYQTFFDEADELLADMEQHLLVLQPEAPDAEQLNAIF
                     RAAHSIKGGAGTFGFSVLQETTHLMENLLDEARRGEMQLNTDIINLFLETKDIMQEQL
                     DAYKQSQEPDAASFDYICQALRQLALEAKGETPSAVTRLSVVAKSEPQDEQSRSQSPR
                     RIILSRLKAGEVDLLEEELGHLTTLTDVVKGADSLSAILPGDIAEDDITAVLCFVIEA
                     DQITFETVEVSPKISTPPVLKLAAEQAPTGRVEREKTTRSNESTSIRVAVEKVDQLIN
                     LVGELVITQSMLAQRSSELDPVNHGDLITSMGQLQRNARDLQESVMSIRMMPMEYVFS
                     RYPRLVRDLAGKLGKQVELTLVGSSTELDKSLIERIIDPLTHLVRNSLDHGIELPEKR
                     LAAGKNSVGNLILSAEHQGGNICIEVTDDGAGLNRERILAKAASQGLTVSENMSDDEV
                     AMLIFAPGFSTAEQVTDVSGRGVGMDVVKRNIQEMGGHVEIQSKQGTGTTIRILLPLT
                     LAILDGMSVRVADEVFILPLNAVMESLQPREADLHPLAGGERVLEVRGEYLPIVELWK
                     VFNVAGAKTEATQGIVVILQSGGRRYALLVDQLIGQHQVVVKNLESNYRKVPGISAAT
                     ILGDGSVALIVDVSALQAINREQRMANTAA"
     gene            complement(16950..17876)
                     /gene="motB"
                     /locus_tag="ACHOSC_RS09840"
                     /old_locus_tag="pgaptmp_001968"
     CDS             complement(16950..17876)
                     /gene="motB"
                     /locus_tag="ACHOSC_RS09840"
                     /old_locus_tag="pgaptmp_001968"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor protein MotB"
                     /protein_id="HGU4547910.1"
                     /translation="MKNQAHPIIVVKRRKAKSHGAAHGSWKIAYADFMTAMMAFFLVM
                     WLISISSPKELIQIAEYFRTPLATAVTGGDRISNSESPIPGGGDDYTQSQGEVNKQPN
                     IEELKKRMEQSRLRKLRGDLDQLIESDPKLRALRPHLKIDLVQEGLRIQIIDSQNRPM
                     FRTGSADVEPYMRDILRAIAPVLNGIPNRISLSGHTDDFPYASGEKGYSNWELSADRA
                     NASRRELMVGGLDSGKVLRVVGMAATMRLSDRGPDDAVNRRISLLVLNKQAEQAILHE
                     NAESQNEPVSALEKPEVAPQVSVPTMPSAEPR"
     gene            complement(17873..18760)
                     /gene="motA"
                     /locus_tag="ACHOSC_RS09845"
                     /old_locus_tag="pgaptmp_001969"
     CDS             complement(17873..18760)
                     /gene="motA"
                     /locus_tag="ACHOSC_RS09845"
                     /old_locus_tag="pgaptmp_001969"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor stator protein MotA"
                     /protein_id="HGU4547911.1"
                     /translation="MLILLGYLVVLGTVFGGYLMTGGSLGALYQPAELVIIAGAGIGS
                     FIVGNNGKAIKGTLKALPLLFRRSKYTKAMYMDLLALLYRLMAKSRQMGMFSLERDIE
                     NPRESEIFASYPRILADSVMLDFIVDYLRLIISGHMNTFEIEALMDEEIETHESEAEV
                     PANSLALVGDSLPAFGIVAAVMGVVHALGSADRPAAELGALIAHAMVGTFLGILLAYG
                     FISPLASVLRQKSAETSKMMQCVKVTLLSNLNGYAPPIAVEFGRKTLYSSERPSFIEL
                     EEHVRAVKNPQQQMTTEEA"
     gene            complement(18887..19465)
                     /gene="flhC"
                     /locus_tag="ACHOSC_RS09850"
                     /old_locus_tag="pgaptmp_001970"
     CDS             complement(18887..19465)
                     /gene="flhC"
                     /locus_tag="ACHOSC_RS09850"
                     /old_locus_tag="pgaptmp_001970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0045893 - positive regulation of
                     DNA-templated transcription [Evidence IEA];
                     GO_process: GO:1902208 - regulation of bacterial-type
                     flagellum assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar transcriptional regulator FlhC"
                     /protein_id="HGU4547912.1"
                     /translation="MSEKSIVQEARDIQLAMELITLGARLQMLESETQLSRGRLIKLY
                     KELRGSPPPKGMLPFSTDWFMTWEQNVHASMFCNAWQFLLKTGLCNGVDAVIKAYRLY
                     LEQCPQAEEGPLLALTRAWTLVRFVESGLLQLSSCNCCGGNFITHAHQPVGSFACSLC
                     QPPSRAVKRRKLSQNPADIIPQLLDEQRVQAV"
     gene            complement(19468..19818)
                     /gene="flhD"
                     /locus_tag="ACHOSC_RS09855"
                     /old_locus_tag="pgaptmp_001971"
     CDS             complement(19468..19818)
                     /gene="flhD"
                     /locus_tag="ACHOSC_RS09855"
                     /old_locus_tag="pgaptmp_001971"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416406.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0045893 - positive regulation of
                     DNA-templated transcription [Evidence IEA];
                     GO_process: GO:1902208 - regulation of bacterial-type
                     flagellum assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar transcriptional regulator FlhD"
                     /protein_id="HGU4547913.1"
                     /translation="MHTSELLKHIYDINLSYLLLAQRLIVQDKASAMFRLGINEEMAT
                     TLAALTLPQMVKLAETNQLVCHFRFDSHQTITQLTQDSRVDDLQQIHTGIMLSTRLLN
                     DVNQPEEALRKKRA"
BASE COUNT         4750 a         5515 c         4890 g         4807 t
ORIGIN      
        1 cactgtcacc cgctcaacag atcacggtta tcgttcgttt tttatactgc tcagggataa
       61 aaaaaggccc ctgttgaaat tgcaggggcc tggtacgagc aagcatcata ttgggcgaca
      121 tgatgcaacg gtaaaaatca tttggcctga tggcgttcaa tgattccttt catttcagca
      181 accgccgccc cgtctacgat ataacgcgaa tactcgtccg cgtgttcatc tgatgacatg
      241 gacaatcctt gattacgata acgcatcggt gaggcttgcc acgctcccgc ggagctatgg
      301 acttccagca ctccggcatc gaggaagtgg tgcaggtttt ctgcacggac tcctgctccg
      361 gccataatga ttggagcatc acgatgggca ataagttcca taatttttga taaaccttgc
      421 agtgcgtctg atttttgccc tgatgtcagt acccgggcaa tgcccaattc cgcaagatta
      481 ttgagtgtat ataaagggtt agcgcacata tcgaaggcgc gatgaaaagt cactgccagc
      541 ggaccggcag cagccattat tttttccatt cgtggcatat cgacattccc gtcaacatcg
      601 agaacgcccg tcaccagtcc aggaaaacct aattcgcgga ccgtgcgcac atcctcaaga
      661 atggcggcaa actcaccgtc gctgtagcaa aaatcaccac cgcgtgggcg aattatcgga
      721 tgcacaggga tcgtcacccg ctggcgcacg gatttcagta cccccaacga cggcgttaag
      781 cccccctctt ttggggctgc gcataattca actctgtctg cgccgttttg ctgcgccgtt
      841 agtgcacatt ccatgctgta acagcaaatt tccagtaatg ccatttttgc tccttaatta
      901 cgctgactgc tcgctggcaa cgatctcttc aatggaccac ggatgaaact taatagttgt
      961 tttgccatcg gtgaccgcca gcgtcggatt cggtaatcgc tcatgctcac cttttggtga
     1021 actggtttta actgagatcc ccggtaggct gagtccttca tcagaaagca atgccagcgc
     1081 acgggcattc agcgtttctg gatcacccgg cagaacgatt tcaatatgtt cccaaccttc
     1141 gtgtgggtaa cgtttttccc cgggccacgg tagctccaca atagaaaact gccagtgcgc
     1201 aacctgtacc ggttcatgca atttaaacag acaaatcggt ctgccattga tcatattttc
     1261 tgacaaaagc tcgccacact gttcaaaccc gcgacgccag cgttcagcag tggcgttttg
     1321 atggcaacgc aaagaaatgt gatcggcagt cagcggagtg atattcaacc ccagacggag
     1381 ggaaagttca tctaatgcgt gggtaaatcg cggtaaatcc gatgcaatat cctgcagctc
     1441 gtcgatagat tgccagttcg ccataatcac tcttcgtctt tcagtaaaag cgttaattta
     1501 ccctgttgcc ctgcgccaac caaccgctga tttcacgccg cttctgatgc aatagtgaaa
     1561 acggcaatac gccacgcgca cgttgctgac gaaaacagcc atttgcagta tactcccgcc
     1621 ctaatttctt taactggtgc gggcaatttt tgctcgcttc atcaatgtaa ggtattccgg
     1681 tgaatattca ggctcttctc tcagaaaaag tccgtcaggc catgattgcg gcaggcgcgc
     1741 ctgcggattg cgaaccgcag gttcgtcagt cagcaaaagt tcagttcggc gactatcagg
     1801 ctaacggcat gatggcagtt gctaaaaaac tgggtatggc accgcgacaa ttagcagagc
     1861 aggtgctgac tcatctggat cttaacggta tcgccagcaa agttgagatc gccggtccag
     1921 gctttatcaa cattttcctt gatccggcat tcctggctga acatgttcag caggcgctgg
     1981 cgtccgatcg tctcggtgtt tctacgccag aaaaacagac cattgtggtt gactactctg
     2041 cgccaaacgt ggcgaaagag atgcatgtcg gtcacctgcg ctctaccatt attggtgacg
     2101 cagcagtgcg tactctggag ttcctcggtc acaaagtgat tcgcgcaaac cacgtcggcg
     2161 actggggcac tcagttcggt atgctgattg catggctgga aaagcagcag caggaaaacg
     2221 ccggtgaaat ggagctggct gaccttgaag gtttctaccg cgatgcgaaa aagcattacg
     2281 acgaagatga agagttcgcc gagcgtgcac gtaactacgt ggtaaaactg caaagcggtg
     2341 acgaatattt ccgcgagatg tggcgcaaac tggtcgacat caccatgacg cagaaccaga
     2401 tcacttacga tcgtctcaac gtgacgctga cccgtgatga cgtgatgggc gaaagcctct
     2461 acaacccaat gctgccagga attgtggcgg atctcaaagc caaaggtctg gcagtagaaa
     2521 gcgaaggggc gaccgtcgta ttccttgatg agtttaaaaa caaggaaggc gaaccgatgg
     2581 gcgtgatcat tcagaagaaa gatggcggct atctctacac caccactgat atcgcctgtg
     2641 cgaaatatcg ttatgaatca ctgcatgccg atcgcgtgct gtattacatc gactcccgtc
     2701 agcatcaaca cctaatgcag gcatgggcga tcgtccgtaa agcaggctat gtaccggaat
     2761 ccgtaccgct ggaacaccac atgttcggca tgatgctggg taaagacggc aaaccgttca
     2821 aaacccgcgc gggtggtaca gtgaaactgg ccgatctgct ggatgaagcc ctggaacgtg
     2881 cacgccgtct ggtggcagaa aagaacccgg atatgccagc tgacgagctg gaaaaactgg
     2941 ctaacgcggt tggtattggt gcggtgaaat atgcggatct ctccaaaaac cgcaccacgg
     3001 actacatctt cgactgggac aacatgctgg cgtttgaggg taataccgcg ccatacatgc
     3061 agtatgctta cacgcgtgta ttgtccgtgt tccgtaaggc agaaattaac gaagagcaac
     3121 tggctgcagc tccggtaatc atccgtgaag atcgtgaagc gcaactggca gctcgcctgc
     3181 tgcagtttga agaaaccctc accgtggttg cccgtgaagg cacgccgcat gtgatgtgtg
     3241 cttacctgta cgatctggct ggtctgttct ctggcttcta cgagcactgc ccgatcctca
     3301 gcgcagaaaa cgaagaagtg cgtaacagcc gtctgaaact ggcacaactg acagcgaaga
     3361 cgctaaagct gggtctggat acgctgggta ttgagacagt agagcgtatg taatcgattt
     3421 ttcgtgagag taaagcctga tcagggttac ctgatcaggc ttttttattg ccatctaaat
     3481 gtattctgaa aatggacatg ccattgtttt ctgactgttg gataagaggc cagaagcgta
     3541 atatccggcc ccagggaaac gataacggtt gaatttaagg aataccgcag tgtttaaatt
     3601 tcttgtatta acattaggca ttatctcttg ccaggcttac gcagaagata cggttatagt
     3661 aaacgaccat gacatttcag ccatcaaaga ttgttggcaa aaaaattcag atgatgatac
     3721 tgacattaac gtgatcaaat catgcctgcg acaagaatac aatctcgtcg atgcgcaatt
     3781 aaataaagcc tatggtgaag cttatcgtta tatagaacaa gtgccacgca caggtgtaaa
     3841 aaaacctgat accgaacaac ttaacttgct taaaaaatca cagcgagcct ggctggattt
     3901 tagggacaaa gaatgtgaat taatcctttc aaatgaggac gttcaggatt taagtgaccc
     3961 ttattctgaa tcagaatggc tctcatgtat gatcatacag accaatacgc gaactcgcca
     4021 gttgcagcta taccgtaact ctgaagattt ttatccaagc cctttgacaa gaggataatt
     4081 cacatctttt tggcatgttt tgttgcaagc tattcctgat aaataattgc aacaagacat
     4141 cgagcctttt tcactgagtt attaaacata ctcgcgagcg cgtaattttt ttgtccttca
     4201 gcgataattc acaatcactt cattacgttg tacctttagc ggtggaatta accgcccacc
     4261 gcctggcact tcccagataa atcgcaacgg ttctgctgcc gctataccgg aaaaggccac
     4321 ggtggttccg ctctgcccct ctaattcgac acaacgagat tgcgaacaca agcgaacccg
     4381 cagtcctgaa ggtgtcgggc cgataagctg ataacgccac gctaccagcg tcatcaatcc
     4441 tgaagcaggt tgtcgcgtag aaagaggcgc agacgacatc gactcaccgc gatgatttag
     4501 cgtaattcct acactacttg cctgccacat cccctccccg gcggcttgca ccagcagcgg
     4561 aaacaataat attgctaata aggttctcat tatttgccgc caattgtcgc cgtcatgcgg
     4621 atatgtcggt tatcagacag ttccagattc gacaggacca ctaactgcgg caagctgcgg
     4681 cgcaggaagc gagacaataa tggtcgcggc gcgtggttca ccaacaatac tggcggcgca
     4741 cccagcatct cctgacggga tagcgcttcc tgagtttgcg ccagtaaacg atccgccagc
     4801 cctggctcca gtcctccccc gccctgcagc gcctgtagta acaaacgttc cagcggtgta
     4861 tcgaggccaa taacatggac ttcatctttg ccaggaaacc actgctgggt aatcgctcgt
     4921 cccaacgcca cgcgcacgac ggcggttaat tcatgtggat cgctttggat gggcgcatgt
     4981 tccgccagcg tttcgagaat ggtgcgcata tcgcgaatcg gtactttttc atcgaggaga
     5041 ttttgcagca ctttatgcag tgtggtgagc gtgacgacgc caggaacgag atcttccgtc
     5101 agctttggca tctcctgggc gacgcgatcc aacagctgtt gcgcctcctg acgaccaaac
     5161 agctctgcgg catgctggct aatgaggtgg ttaagatgcg ttgctaccac cgtgctggcc
     5221 tcaaccactg tgtacccctg aatctgcgcc tgttctttta gcgcactttc aatccagata
     5281 gcattcaggc caaatgccgg atcgacggtc gcctcaccag gtaacgtccc ggcagcggtt
     5341 ccagggttaa tcgccagcca gcgccccgga taagcatcac cactgccaat ctccacgcct
     5401 ttcatcaaaa tgcgatagcg ggcaggttgc agctccatat tgtcgcgaat gtgcaccact
     5461 ggcggcagaa atcccatctc ctgggcaaat ttcttgcgga tactgcgtat acggcccaac
     5521 aactcaccat cctgctggaa atcgaccatc gggatcagtc gataacccac ttccattccc
     5581 agagaatctt ccagttgtac atcgttccac gtcgcttcga caacggtatt attctctgcc
     5641 atttttaccg gtttgggttc gacaggcgct ttttgttcgc gtccgcgtat ccaccaggcc
     5701 agccccagca atccggcagt gaacagcaaa aataccaggt tcggcattcc aggcaccagg
     5761 ccgagtaaac cgagcacggc ggcgcttaac aacataacgc ttgggttact gaaaagctga
     5821 ttcaccatct gctcgccaac atcctgatcg gtgctgacac gcgtaacgat gacccccgcg
     5881 gcggtagaaa tcaccagcgc cggaatttgt gccaccagac cgtcgccaat ggtcaatagc
     5941 gtataacttt ccgccgcgtg tcccatgctc atgccatgtt gcagcacgcc gaccagcaac
     6001 ccgccgacaa cgttaatgac catgatgagg atcccggcga tggcatcgcc gcgaacaaac
     6061 ttacttgccc cgtccattga gccgtaaaaa tcggcttcct gagtcacttc ggagcggcgt
     6121 tttttcgcct catcttcacc aatcaatcca gcgttaaggt cggcgtcaat cgccatctgc
     6181 ttacccggca taccatcgag aacaaagcgc gcacccactt ctgcgatacg cccggcacct
     6241 ttggtaatga ccataaagtt gatgatcacg agaatgacaa acactacgat accgatagcg
     6301 aaattgccac caacgaggaa gtgaccgaat gcttcgacca ccttcccagc cgccgccgcg
     6361 ccggtatgcc cttccatcaa aatgatacgg gttgaagcca cgttaagtgc cagacgcaac
     6421 aacgtagtaa acaacagaat ggtcggaaac gcagcaaact caagcgtgcg ctgggtaaac
     6481 atcgccacca gcaacaccat gatcgacaag gcaatattga aggtaaacaa caggtcgagt
     6541 atgaatgcgg gcagtggcag caccatcatc gacaagatca acaggatcaa aatcggtccg
     6601 gcaaggatct gccattgtgt cgatttcagg tttgcgggca ggcgcagcat cgcggccaga
     6661 ttactcatgg gtcggtttct cgttaataaa atccagggct tccggcaccg gaagatgagt
     6721 aggttgtaca gggcgctgtc caccagccag acgccagcgt ttcagttgcc agacccaggc
     6781 cagcacttcc gccaccgcgg cgtacagttg acccgggatt tgttgaccaa tctccgcatg
     6841 tcgatacagc gctcgcgcca gcggcggcgc ttcaagcgtc gggacgttat tttcagcgcc
     6901 aatttcacga atgcgcagcg cgaccagccc tgcaccttta gcgaccactt tcggtgcgct
     6961 cattttgttt tcgtcatact gcaacgctac cgaatagtgg gtcgggttat tgacaatgac
     7021 atccgctttc ggcacatcgg ccatcatccg acgccgtgca gcagctcgct gcatctgacg
     7081 gatccgccct ttaacatgag ggtcaccttc gctttgtttg aactcatcac gaatatcctg
     7141 ccgtgacata cgcagctttt tcaggtggct gaatatttgg aaaaagacgt caaatcccac
     7201 cattggaatg acaccaagca ccaccagcag tgcgcatagc cctaccaaat ccatcgcatt
     7261 acccatggcg gtaatcggag actcggccat caagcgcatc atctgcggcc agtgatgcca
     7321 gagaaaaaac cccgtcacgc tgccaaccag gatggttttc aaaattgctt taagcaactc
     7381 cgcgccagtc tgagccgaga acatccgttt aatgcccggt agcgggttga gtttggaaaa
     7441 cttcggctgc aaggatttgc cgctaaatac cagccctccc agcatgaccg gagaaatgag
     7501 cgccaccagc accacgccgc taatcagcgg cagcagcgcc agcatggctt ctctgatcag
     7561 cagaataatc tgcccgagga tcagattcgg gtcattgata atactgtgat caaaatgcag
     7621 cccagcggag agcatgcccg acaatcgacg ggccagcgac acaccgccaa accagataac
     7681 actaacgccc accagcaaaa tcagcagtga ggtcagttca cgggaacgcg ggatttgccc
     7741 ctcttcccgc gctttttcta gtcggtgagg tgtgggggct tctgttttgt cgtcgctctc
     7801 gtcagacacg tcgccaatcc tggaaagagt attaagcgtg aatgatgcca gagcgcaaag
     7861 cgttcaatgg tttgagtaag gggcaaaaca ggcgggattt agggcttttg ctgccacaca
     7921 tcaagcatag tgtgcgtttg tcggatgcgg cgtcatcgcc ttatcagacc gcctgatatg
     7981 acgtggtcac gccacatcag gcaatacaaa tcaaaatcca agactatcca acaaatcgtc
     8041 cacctgatcc tgactggcta ccacaccggc tttgctggta tcgacctgag gtccattaag
     8101 caaactctgg ttttcacgtt ttggacgcga ctcctgttcc gggatgtttt ccaacagcac
     8161 catcagcaac tggcgttcga tctcctgaat gacatccatc atccgcttaa tgacctgccc
     8221 ggtgagatcc tgaaaatcct gcgccatcat gatttccagc agttgcgcgt tagtaaagct
     8281 ggtatgcgcg ggtacatctg ccagaaattg tcgtgtatct gttaccagtt cacgggcgtc
     8341 ggcaaggtca atcggatcgg caaaccagtc atcccaacgt tgggttaacg cttttgctga
     8401 tttctccatt tgatcctgat gcggttgtga cgcctcaaca ctgttcagcg cccgctccgc
     8461 agcctgggcg gtcatctgca caacatagta caaacgatcg cgcgcatcgg ggatggcttc
     8521 cgccgcttcg gcaatggcct gatccagccc cagttcccgc aaactgtcgc gcagcatacg
     8581 cgtcaggctg ccgatgcgcg caatgatatc gccagctgaa tgctcgtcag caggtttgat
     8641 tgatggttgc atcatagtcg catcctcaca tgcccagttt ctcaaagatt ttgttgagtt
     8701 tttcctccag cgtcgcggcg gtaaatggct tcaccacata gccactggcc cccgcttgcg
     8761 ccgcagcaat gatgttctct ttcttcgctt ctgcagtcac cattaacact ggcaatgccg
     8821 acatcgcgcc atccgcacga attgttttca gcaattccag gccatccata ttgggcatgt
     8881 tccagtcgga gataacaaat ccataaccgc ctgcctgcaa cttattgaga gcgtcgacgc
     8941 catcttccgc ttcctcaaca ttattgaatc ccagctcttt cagcaggtta cgcactatgc
     9001 gtcgcatggt ggaaaagtca tccacaacca aaaatttaag ttctttatcc gccatttcac
     9061 actcctgatt taaatacgta tcgcctgtcc ggcactaatt tttgccaaca tttgctggct
     9121 tacctggcta agatcgacca cttcgcagac accacccata ttgatggcct cgcgcggcat
     9181 gccgaacacc acgcaacttg cttcgttttg cgcaagggtc catgcccccg cctgacgcat
     9241 cgccaacatt cccgccgcgc cgtcgttgcc cataccggtc aggatcaccc caaccgcatt
     9301 acgccccgcc tgtttggcga cagaatggaa caacacatct accgaaggcc gatgacggtt
     9361 aaccgccggg ccatcgtgaa ttttgatttg gtaatttgcg ccactacgcg acagctccat
     9421 atgccgatcg cccggcgcaa tataggcatg ccccggcaag acacgttctc cgtcttcggc
     9481 ttctttaacc ccgatctggc aaagcttatt aagtctgtcg gcaaaagagc gggtgaaacc
     9541 gggcggcata tgttgggtaa ttaacaatgc cgggctggaa agcggcaacg gttgcagtac
     9601 gtgacgaatt gcctcagttc cacccgttga agcaccaatc gcaatcagtt tttcagaact
     9661 caacaacgga cccgccttca gcgttgtcgg tgctgacaat ggcttatgtg ctgcaaggct
     9721 cgcctttgct gccgtacgca ccttttcagc aatcatttcg ctatacgcca gcattccttc
     9781 gcgaataccc agttgcggtt tggtgacaaa atctattgcc cccagctcca gcgcgcgcag
     9841 cgtgacttct gatcctttgc cggtcaagga agaaaccatc acaacgggca ttggacgcaa
     9901 acgcattaat ttttcgagga aatccagtcc gtccatccgc ggcatttcaa catccagcgt
     9961 cagcacatcg ggattgaatt tcttaatcaa gtcacgcgcg accagcggat caggcgcggt
    10021 cgccaccatt tccatgtcgc tatggctgtt gatgatttct gtcatgatct ggcgcatcag
    10081 tgccgaatca tcgacagata acaccctgat tttgctcatc gttaatcctt acttagcgca
    10141 tacaccgtct gaccacgcag cgtgaagcgg cgctcaaggt ggctaaagtt ttcagagtga
    10201 cccgcaaaca gcaatccgtc gggtttaagg agcggaacaa agcggcgcaa aatctcctgc
    10261 tgggtatttt gatcgaagta gatcatgacg ttacgacaga agatcgcatc aaacggcccc
    10321 ggcacggtgt actgtttcgc cagtagattc agcggggcaa aatcaacata gttcgccagc
    10381 tcctgacgca cgcgtaccag cccttcatgc ggtcccgtcc ctcgcatgaa ataccgttgc
    10441 agttgctgcg gcgtcaggtt tttcaactct tcatggcgat aaataccgct tctggctttt
    10501 tccagcactt cggtgtcgat atcactggca aacactttcc agcgtccggg cgcggtgccc
    10561 aatgtgtcag ccagcgtcat cgcaatgctg tacggctctt cgccggtcga agccgccgcg
    10621 ctccatacgc gatactcgcc agaacggcga cgtgcgtgat ccgcgagcag agggaaatgg
    10681 tgtgcctcac ggaaaaatgc cgtcagattc gtggtcagcg aattgataaa cgcctgccac
    10741 tcaccgctgt gctgattaga ttccagcaag ttcagataat gaccgaaatc cgtcagtccc
    10801 agcgaacgca aacgacgaac cagtcggttg taaaccatgt cgcgtttatg gtcagccaga
    10861 acgatcccgg ctcgttgata gatcaattga cttatccgcc gaaaatgcgc gtcggaaagc
    10921 gccaggcgct cggtcatctg taacaataaa gacgtttgcc cacagggcag agatgaagtc
    10981 atagcgcctt ctcaatcact tcaggatacc actggcgcaa tttgtaactg cgccgactca
    11041 tgtcgtgcta cttcatgttc ttcaagggta aataccgcca cacgcgacga aagatggtcg
    11101 gcctgattcg ccagttgttc cgttgccacc gccgcttctt ctaccagcga ggcgttctgc
    11161 tgagtcacct gatccatctg gctgacagcc tgtgcaacct gctcaatccc ccgctgttgt
    11221 tcttccgacg cagaggcaat ttctcccata atgtcgtcca cgcgggtcac cgaactgaca
    11281 atatcgatca tggtcgcggc ggcgttattc accagtttcg aaccctgctg gacacgattg
    11341 actgactctt cgatcagccc tttgatctct tttgctgcct gcgcgctacg gctggcaaga
    11401 ttgcgcactt cacctgccac taccgcaaaa ccacgcccct gctctccggc gcgagccgct
    11461 tccactgccg cattcagggc cagaatattg gtctggaaag caattccgtc gataacgctg
    11521 ataatgtcgc caattttttg cgagctggtg gcgatctcct gcatggtgtg agtcatggta
    11581 ctgacctgaa caccgcccgc ctgcgccgtt gtcgcggcat tttttgccag ttccgacgcc
    11641 tgtcgtgcgt tatcggcgtt ttgccctacc gtggcggtta attgctccat actggcggcc
    11701 gtttgtgcca gcgatgccgc ctgctgttcg gtacgacttg agagatcgtt attgcctgcg
    11761 acaatctccg cgataccaat gtgcatctcc tggcttccct tacgcacatc actcaccgtc
    11821 ccacgcaaag cctgctgcat ggtcttcagg ctggcaaaaa tggcggtgat ctcattacga
    11881 ccatataccg caatcggacg cgccagatta cccgcagcaa tgctgtcaaa atggctaccg
    11941 ataatggcca gtggttgaac aatcatcttg cgcgtccacc acagcgcact gctgatgtag
    12001 atcgccgcaa caataatcat gctgataaac accagcgccg aaatctgata gttacgctgg
    12061 ctttgcgcac tggcggcttc cagcacatgg ttgatctcca gctgccaggc ctcaaagtta
    12121 acgtcaaacg cattctgtga ttcctgcacc ggcgcagtga ggaaatccga aagttgatta
    12181 ctttcaagcc aggtcgcctg atgttcgaga tcgttgttgt gccagcgggc aaaacttttc
    12241 tccgtctctt tttgcaacgc cctgacgtgc tcgttgcccg cagtcatcgc cataaaactt
    12301 ttaaacagcg tggtggattg cgtcagactg gcgcgcgccg tcgtcatcaa cgttttaatg
    12361 tcatccgccg gatagctaag tgccgtcaga gtgcccgctt tgttcagcgc ggtactggcc
    12421 tgtaacatta ccgcccgagt ttgcgctaat gccgcacgtt gctgattgct ctgctcgacc
    12481 tgattcagtc gttgcaaatc gtcgcgaaat gcccaaaaag acatgccgtt actgccaatc
    12541 tgcaagatcc cgcagagaat caaaattaaa aacagcgtgg tcgaaattcg aatacgatta
    12601 aacatcaacg ctccccatca ggcggcaatg accgcgttag taaatactcg tcaaaatgtt
    12661 tcccagtttg gagcttgttc agcaattcgc agtcgtggct gtgccggtgg ttgctcactg
    12721 gcaggacggg atggtgtttg cggtttattg gtgagtgggc tggctgccag acggaacgcg
    12781 gaaaccgctt gcgttaaacg actcgcctgt tcttccagcg cagcggcggc ggcagctgat
    12841 tcctgcacca gcgatgcgtt ctgttgcgtg acgcgatcca tttccgaaac cgccaatgcg
    12901 acttgatcga tgccacggct ctgttcatcc gatgccgatg caatctcgcc cataatgtca
    12961 gtcacgcgag tgacagcatt gacgatattg ttcattgttt ccccggcgct ttcgaccagc
    13021 accgaaccgg tatcaacgcg tgagacggag tcttcaatga gggctttgat ctcttttgcc
    13081 gcctgggcgc tgcgactggc aagattacgc acttcacccg ccaccacggc aaaaccacgg
    13141 ccctgttcac ccgcacgcgc ggcttcaacc gcggcattca gcgcgaggat attagtctgg
    13201 aaggcaatac cgtcgataac gctgataatg tcggcaattt tcttcgaact atcggcgatc
    13261 tcatgcatcg ttttcactac gccatccacc actttgccgc cgtgctgggc ggtgtcggag
    13321 gcactttgcg ccagttgcga ggcctggcgg gcgttatcgg cgttttgctt cactgtcgcg
    13381 gtgagctgct ccatgctggc ggcagtttct tccagcgcgg atgcctgctg ttcagtacgg
    13441 gaggaaagat cggtgttgcc cgccgcaatt tcacgggtac cggcatagat ggcatctgaa
    13501 ccttcgcgga catgagtgac ggtgtcagtc aaagagcgtt gcatatgtga aacgctctgc
    13561 gccaggtcgc ccatttcact gcgcccgtca atggtcaggg tattcgccag gttaccaccg
    13621 gcgatttcgc gaatgtgagc aataattttt gccagcggag taagcaacat acggcgaatg
    13681 ccgtaccacg ccaccagcag aatcaatacc accaccagcg cgataaccgc cagttgccac
    13741 tgggcaaatc ggtaatcatc tgcgttgtca gtgacgatat cgcgatacag tttttcactg
    13801 ctgagggcgt actgagcaaa cgcttcgccc attgcatttt gcattccctg ggttggctga
    13861 gcgaaataag ctccagtatt gccataatca agataatcaa tcagttcagt taacgctgtg
    13921 tgatagtttt tatatttttc atcaatatta cgactggtag cgaccatttc aggtaacggt
    13981 gccatgcttt tgaatttttt ataatgagtc gcggcctggg ccaatgtttt cctggcgcta
    14041 tcgagcaatt caactttggc gttactttgt tgattggagg aatccatcat catccgcacc
    14101 gctgaacgac tcaggttaat gcgcgtttgc agcattaaat cccaggttga cgtcagctcg
    14161 ccctgctgtt cccgtaattg attggaaacc acaaagctct tctggctatg gtgaagggaa
    14221 gaaaaaaaca gactgccgga aataagctgt aacagtgcga atacccccag caccattacc
    14281 aacagcgtga ctacgcggat acggttaatc ataaggcacc ttcctgaaaa caagttaatc
    14341 tcgttatcgg caagaagggg ggaaacttta ttgctgatgc cacccgccgc gaaattgaaa
    14401 taaaaaaccc gatgcgcaga tcatcgggtt catttcaatt gaggaaatcg ggagaattac
    14461 gccacttctg acgccgcgct atctaacagc gccatctctt cgctgttcag cagtttttcg
    14521 atgttcacca gaatcaacat ccggtcgccc agtgcgccca gtccagtgag atattctgtt
    14581 gaaagcgtca cggcaaactc tggtgccgga cgaatttgct ccgccgtcaa tgaaagcacg
    14641 tctgaaacgc cgtcaaccac gatgccgacc acccgctgtc cgagattcag gacgataact
    14701 accgtgttgt cgttatagtc cacatccacc tggctgaact taattcgtaa gtcaacaatc
    14761 ggcacaataa cgccgcgcag attcgtgacg cctttgataa acgctggcgt gttcgcaatc
    14821 cgtgttacct gatcgtagcc acggatctcc tgcactttca ggatatcaat accgtactct
    14881 tcatcaccaa gggtaaatac cagaaattcc tggcctgacg gctcgctggc cagctttgtt
    14941 acattcgtca taccggtcat attgttacct ttttactcat tcaggcggcg gtgttcgcca
    15001 tacgttgttc gcggtttatc gcctgcaagg cggagacatc aacaatcagt gccacgctgc
    15061 cgtcgccaag aatggtcgca gcagaaatgc cggggacttt gcgatagtta ctttcaaggt
    15121 ttttaaccac aacctggtgc tgaccaatta attgatccac cagcaaggcg tagcggcgac
    15181 cgccactttg taagatcacc acaattccct gggtggcttc ggttttcgcg cccgcgacgt
    15241 tgaacacttt ccacagttcg acgatgggca gatattcacc ccgcacttcc agcacccgct
    15301 cgccgccggc cagtggatgc agatcggctt cacggggttg cagtgattcc ataacagcat
    15361 tcagcggcag aatgaaaact tcatccgcaa cgcgtacgga catgccgtcg aggatggcca
    15421 gcgtcagcgg cagtaaaatg cggatcgtag tgccagtacc ctgcttcgac tggatttcga
    15481 catgaccgcc catctcctgg atattacgtt taacgacgtc catgccgacg ccgcgcccgg
    15541 agacgtcggt gacctgctct gccgtggaga agcctggtgc aaatatcagc atcgcgactt
    15601 cgtcgtcgct catgttttcg ctgacagtca aaccttgcga ggccgctttt gccagaattc
    15661 gctcacggtt tagccccgcc ccatcgtcgg tcacttcaat gcaaatgttg ccgccctgat
    15721 gttcggcaga cagaattaaa tttccgacgc tgtttttacc tgcggcgagc cgtttttctg
    15781 gcagttcaat accgtgatcg aggctattgc gtaccaggtg ggtcagcggg tcgataatgc
    15841 gttctatcag gcttttgtcg agttcagtag aactgcccac cagcgtcagt tctacctgct
    15901 tgccgagttt tcccgccaga tcacgcacca gccggggata gcgactaaaa acatattcca
    15961 tcggcatcat gcgaatcgac atcactgatt cctgcaaatc acgggcgtta cgttgtaact
    16021 gccccatgct ggttatcaaa tcaccatgat taaccgggtc cagttcgctg gaacgctggg
    16081 caagcatgga ctgggtgata accagctcgc cgacgaggtt aattaattga tcaacctttt
    16141 ctaccgctac acggatgctg gtggattcat tgctgcgcgt cgttttttcc cgctccacgc
    16201 ggccggttgg cgcttgttcg gctgccagtt taagcactgg tggggtggat atttttggcg
    16261 agacttctac tgtttcaaag gtaatctgat cggcttcaat cacaaaacag agtaccgctg
    16321 tgatgtcatc ttcggcgatg tcgcccggta atattgccga gagcgaatcc gcccctttca
    16381 ccacgtcagt taacgttgtc agatgtccca gttcttcttc cagcaggtcg acttccccgg
    16441 ccttcaggcg cgaaaggata attcgtcgcg gcgactgact gcgactctgc tcatcttgcg
    16501 gttcactttt ggcaaccaca cttaatcggg tcactgcgga tggcgtttcg cctttcgctt
    16561 ctaatgccag ttgacgcaag gcctggcaga tataatcgaa gctggcggca tccggctctt
    16621 gcgactgttt ataagcgtcg agctgttctt gcatgatgtc cttcgtttcc aaaaacagat
    16681 tgataatgtc ggtgttgagt tgcatctcac ctcgtctggc ttcatcgagc aggttttcca
    16741 tcagatgcgt ggtttcctgc aaaacgctga agccaaaagt tcctgcccct cctttgatcg
    16801 agtgggcagc ccgaaagatg gcattcaatt gttcggcatc tggcgcttcc ggctgcaaaa
    16861 ccagcagatg ctgctccatg tcagccaaca gttcgtccgc ttcatcaaaa aatgtctgat
    16921 aaaaatcgct tatatccatg ctcacgctgt cacctcggtt cggctgatgg cattgtggga
    16981 acactgacct gtggtgcaac ctcaggtttt tccagggcgc ttactggctc attctggctt
    17041 tcggcgtttt catgcaaaat ggcctgttcg gcttgtttgt tcagtaccag caggctgatg
    17101 cgacggttga cggcatcatc aggtccgcga tcgcttaagc gcatcgttgc cgccatgccg
    17161 acgacacgta acactttgcc gctatccaac cctccgacca tcagttcgcg gcgggatgca
    17221 ttggcccgat cggcagaaag ctcccagttg ctatatcctt tctcaccgct ggcgtagggg
    17281 aaatcatcgg tatgacctga aaggctaata cggttgggaa taccgttcag tacaggcgca
    17341 atggcacgca gaatgtcgcg catatagggt tcgacatcgg cactgccggt tctaaacatc
    17401 gggcgattct ggctatcgat gatctgaata cgtagacctt cctggaccag atcgattttg
    17461 agatggggac gtaacgcccg cagtttcgga tcggactcta tcaactggtc gagatcaccc
    17521 cgcaatttcc gcaatcgact ttgctccatg cgttttttca gctcttcgat gttcggctgc
    17581 ttattcactt ccccctggct ttgggtgtaa tcatcaccac cgccgggaat tgggctttca
    17641 ctattagaaa tgcgatcgcc gcccgtaacc gcagtcgcca gtggagtccg gaagtactcc
    17701 gcaatctgaa tcagctcttt tgggctggag atggagatca gccacatcac cagaaaaaag
    17761 gccatcatcg cagtcataaa gtcggcataa gcaatcttcc acgatccatg tgctgccccg
    17821 tggcttttgg ctttgcgtcg tttgacgaca ataatcggat gcgcttgatt cttcatgctt
    17881 cctcggttgt catctgttgt tgcggatttt tcaccgcacg cacatgctct tccagttcaa
    17941 taaacgacgg acgttcgctg gaatagagcg ttttgcgacc aaactcaacg gcgataggcg
    18001 gtgcgtaacc gttcagatta gaaagcagag tgactttgac gcactgcatc attttgctgg
    18061 tttcggcgct tttctgacgt aaaacactcg ctaatgggga aataaatccg taagccaata
    18121 aaatgccgag gaaagtcccc accatcgcat gtgcgataag cgcacccagc tcggcggcag
    18181 gacgatcggc tgaacctaac gcgtgaacga cccccattac agccgcgaca ataccaaacg
    18241 ccggaagtga gtccccgacc agcgccagac tgttcgccgg gacttctgcc tcgctttcgt
    18301 gcgtctcaat ctcttcatcc atcagggctt cgatttcgaa ggtgttcata tgaccgctga
    18361 taatcagacg cagataatcg acgataaaat caagcatgac gctatccgcg aggatgcgtg
    18421 ggtagctggc gaagatctcg ctctcacggg gattttcaat atcacgttcc agcgaaaaca
    18481 tccccatctg ccgcgatttc gccatcaacc gataaagcag agccagcaga tccatataca
    18541 ttgctttggt gtatttggag cgacgaaaca gcaacggcag cgccttcagc gtgcctttaa
    18601 tcgctttgcc attattgccg acgataaacg acccaatccc tgcaccggca ataatcacca
    18661 gttcagcggg ttgatagagt gctccaaggc ttccaccggt catcaaataa ccgccgaaaa
    18721 ctgtaccgag aacaaccagg taacctaata agataagcac gacatcatcc ttccactgtt
    18781 gaccatgaca ggatgttcag tcgtcaggcg ttaacgcgcg attggggcaa aaaaaagcag
    18841 cggtacgtcg ttaccgctgc tggaatgttg cgcctcaccg tatcagttaa acagcctgta
    18901 ctctctgttc atccagcagt tgtgggataa tatcggcagg attctgggaa agtttacgtc
    18961 tttttactgc ccgggatggc ggttgacata agctgcaggc aaagctgcca acaggctggt
    19021 gagcgtgggt aataaaattg ccgccgcagc agttgcagct ggaaagttgc agtaatccac
    19081 tttcaacaaa ccgcaccaat gtccaggcac gggttaatgc cagcagtggt ccttcttctg
    19141 cttgtgggca ctgttcaagg tataaacggt aggctttgat caccgcatcg acgccattac
    19201 acaaaccggt tttcagtaaa aactgccatg cattacagaa catcgaagca tgaacgtttt
    19261 gttcccaggt cataaaccag tcggttgaga atggcagcat gcctttcggc ggtgggcttc
    19321 cgcgcagttc tttataaagt tttatcaggc gtccgcgact taactgtgtt tcgctttcca
    19381 gcatctgcaa acgagcgccc agggtgatca attccattgc cagctgaata tcccgcgctt
    19441 cctgaacaat gcttttttca ctcatgatca ggcccttttc ttgcgcagcg cttcttcagg
    19501 ctgattaaca tcattcagca agcgtgttga gagcatgatg ccggtatgaa tttgctggag
    19561 atcgtcaacg cgggaatctt gcgtcaactg agtaatcgtc tggtggctgt caaaacggaa
    19621 gtgacaaacc agttgattgg tttctgccag cttaaccatt tgcggaagag tcagtgccgc
    19681 taacgttgtc gccatttctt catttatgcc gagacgaaac atagcggacg ctttgtcctg
    19741 aacaatcaaa cgctgtgcaa gtagtaaata tgacaagttg atgtcataaa tgtgtttcag
    19801 caactcggag gtatgcatta ttcccaccca gaataaccaa ctttattttt atgcggtttc
    19861 accgcacccc gtgatgtcgc cgggaagccc cggtaaaaaa taattagcat tagaatagtt
    19921 gcgataagct gcaataagca gaaccacctt tttggtttaa ta
//
LOCUS       DAZKHF020000067        19868 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2201S31241
            SAMN43267800-rid25106413.denovo.067, whole genome shotgun sequence.
ACCESSION   DAZKHF020000067 DAZKHF020000000
VERSION     DAZKHF020000067.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267800
            Sequence Read Archive: SRR30307263
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 19868)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19868)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 19868)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350581.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350581.2
            Genome Coverage       :: 151x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:21:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,652
            CDSs (total)                      :: 5,557
            Genes (coding)                    :: 5,320
            CDSs (with protein)               :: 5,320
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 237
            CDSs (without protein)            :: 237
            Pseudo Genes (ambiguous residues) :: 0 of 237
            Pseudo Genes (frameshifted)       :: 102 of 237
            Pseudo Genes (incomplete)         :: 130 of 237
            Pseudo Genes (internal stop)      :: 52 of 237
            Pseudo Genes (multiple problems)  :: 41 of 237
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19868
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267800-rid25106413.denovo.067"
                     /serotype="O 26:H11"
                     /isolate="2201S31241"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-01"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            complement(365..1561)
                     /gene="hemY"
                     /locus_tag="ACHOSC_RS09860"
                     /old_locus_tag="pgaptmp_001972"
     CDS             complement(365..1561)
                     /gene="hemY"
                     /locus_tag="ACHOSC_RS09860"
                     /old_locus_tag="pgaptmp_001972"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0042168 - heme metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protoheme IX biogenesis protein HemY"
                     /protein_id="HGU4547914.1"
                     /translation="MLKVLLLFVLLIAGIVVGPMIAGHQGYVLIQTDNYNIETSVTGL
                     AIILILAMVVLFAIEWLLRRIFRTGAHTRGWFVGRKRRRARKQTEQALLKLAEGDYQQ
                     VEKLMAKNADHAEQPVVNYLLAAEAAQQRGDEARANQHLERAAELAGNDTIPVEITRV
                     RLQLARNENHAARHGVDKLLEVTPRHPEVLRLAEQAYIRTGAWSSLLDIIPSMAKAHV
                     GDEEHRAMLEQQAWIGLMDQARADNGSEGLRNWWKNQSRKTRHQVALQVAMAEHLIEC
                     DDHDTAQQIIIDGLKRQYDDRLLLPIPRLKTNNPEQLEKVLRQQIKNVGDRPLLWSTL
                     GQSLMKHGEWQEASLAFRAALKQRPDAYDYAWLADALDRLHKPEEAAAMRRDGLMLTL
                     QNNPPQ"
     gene            complement(1564..2793)
                     /gene="hemX"
                     /locus_tag="ACHOSC_RS09865"
                     /old_locus_tag="pgaptmp_001973"
     CDS             complement(1564..2793)
                     /gene="hemX"
                     /locus_tag="ACHOSC_RS09865"
                     /old_locus_tag="pgaptmp_001973"
                     /EC_number="2.1.1.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen-III C-methyltransferase"
                     /protein_id="HGU4547915.1"
                     /translation="MTEQEKTSAVVEETREAVDTTSQPVATEKKSKNNTALILSAVAI
                     AIALAAGVGLYGWGKQQAVNQTATSDALANQLTALQKAQESQKAELEGIIKQQAAQLK
                     QANRQQETLAKQLDEVQQKVATISGSDAKTWLLAQADFLVKLAGRKLWSDQDVTTAAA
                     LLKSADASLADMNDPSLITVRRAITDDIASLSAVSQVDYDGIILKLNQLSNQVDNLRL
                     ADNDSDGSPMDSDGEELSSSISEWRINLQKSWQNFMDNFITIRRRDDTAVPLLAPNQD
                     IYLRENIRSRLLVAAQAVPRHQEETYRQALENVSTWVRAYYDTDDATTKAFLDEVDQL
                     SQQNISMDLPETLQSQAMLEKLMQTRVRNLLAQPAAGTTEAKPAPAPAPAPAPAPAPA
                     PAPAPQGDTPAAAPQGE"
     gene            complement(2815..3555)
                     /gene="hemD"
                     /locus_tag="ACHOSC_RS09870"
                     /old_locus_tag="pgaptmp_001974"
     CDS             complement(2815..3555)
                     /gene="hemD"
                     /locus_tag="ACHOSC_RS09870"
                     /old_locus_tag="pgaptmp_001974"
                     /EC_number="4.2.1.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004852 - uroporphyrinogen-III synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006780 - uroporphyrinogen III biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen-III synthase"
                     /protein_id="HGU4547916.1"
                     /translation="MSILVTRPSPAGEELVSRLRTLGQVAWHFPLIEFSPGRQLPQLA
                     DQLAALGESDLLFALSQHAVAFAQSQLHQQDRKWPRLPDYFAIGRTTALALHTVSGQK
                     ILYPQDREISEVLLQLPELQNIAGKRALILRGNGGRELIGDTLTARGAEVTFCECYQR
                     CAIHYDGAEEAMRWQSREVTMVVVTSGEMLQQLWSLIPQWYREHWLLHCRLLVVSERL
                     AKLARELGWQDIKVADNADNDALLRALQ"
     gene            complement(3552..4493)
                     /gene="hemC"
                     /locus_tag="ACHOSC_RS09875"
                     /old_locus_tag="pgaptmp_001975"
     CDS             complement(3552..4493)
                     /gene="hemC"
                     /locus_tag="ACHOSC_RS09875"
                     /old_locus_tag="pgaptmp_001975"
                     /EC_number="2.5.1.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004418 - hydroxymethylbilane synthase
                     activity [Evidence IEA];
                     GO_process: GO:0033014 - tetrapyrrole biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylbilane synthase"
                     /protein_id="HGU4547917.1"
                     /translation="MLDNVLRIATRQSPLALWQAHYVKDKLMASHPGLVVELVPMVTR
                     GDVILDTPLAKVGGKGLFVKELEVALLENRADIAVHSMKDVPVEFPQGLGLVTICERE
                     DPRDAFVSNNYDSLDALPAGSIVGTSSLRRQCQLAERRPDLIIRSLRGNVGTRLSKLD
                     NGEYDAIILAVAGLKRLGLESRIRAALPPEISLPAVGQGAVGIECRLDDTRTRELLAA
                     LNHHETALRVTAERAMNTRLEGGCQVPIGSYAELIDGEIWLRALVGAPDGSQIIRGER
                     RGAPQNAEQMGISLAEELLNNGAREILAEVYNGDAPA"
     gene            4880..7426
                     /gene="cyaA"
                     /locus_tag="ACHOSC_RS09880"
                     /old_locus_tag="pgaptmp_001976"
     CDS             4880..7426
                     /gene="cyaA"
                     /locus_tag="ACHOSC_RS09880"
                     /old_locus_tag="pgaptmp_001976"
                     /EC_number="4.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004016 - adenylate cyclase activity
                     [Evidence IEA];
                     GO_process: GO:0006171 - cAMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I adenylate cyclase"
                     /protein_id="HGU4547918.1"
                     /translation="MYLYIETLKQRLDAINQLRVDRALAAMGPAFQQVYSLLPTLLHY
                     HHPLMPGYLDGNVPKGICLYTPDETQRHYLNELELYRGMSVQDPPKGELPITGVYTMG
                     STSSVGQSCSSDLDIWVCHQSWLDSEERQLLQRKCSLLESWAASLGVEVSFFLIDENR
                     FRHNESGSLGGEDCGSTQHILLLDEFYRTAVRLAGKRILWNMVPCDEEEHYDDYVMTL
                     YAQGVLTPNEWLDLGGLSSLSAEEYFGASLWQLYKSIDSPYKAVLKTLLLEAYSWEYP
                     NPRLLAKDIKQRLHDGEIVSFGLDPYCMMLERVTEYLTAIEDFTRLDLVRRCFYLKVC
                     EKLSRERACVGWRRAVLSQLVSEWGWDEARLAMLDNRANWKIDQVREAHNELLDAMMQ
                     SYRNLIRFARRNNLSVSASPQDIGVLTRKLYAAFEALPGKVTLVNPQISPDLSEPNLT
                     FIYVPPGRANRSGWYLYNRAPNIESIISHQPLEYNRYLNKLVAWAWFNGLLTSRTRLY
                     IKGNGIVDLPKLQEMVADVSHHFPLRLPAPTPKALYSPCEIRHLAIIVNLEYDPTAAF
                     RNQVVHFDFRKLDVFSFGENQNCLVGSVDLLYRNSWNEVRTLHFNGEQSMIEALKTIL
                     GKMHQDAAPPDSVEVFCYSQHLRGLIRTRVQQLVSECIELRLSSTRQETGRFKALRVS
                     GQTWGLFFERLNVSVQKLENAIEFYGAISHNKLHGLSVQVETNHVKLPAVVDGFASEG
                     IIQFFFEETQDENGFNIYILDESNRVEVYHHCEGSKEELVRDVSRFYSSSHDRFTYGS
                     SFINFNLPQFYQIVKVDGREQVIPFRTKSIGNMPPANQDNDTPLLQQYFS"
     gene            complement(7466..7786)
                     /gene="cyaY"
                     /locus_tag="ACHOSC_RS09885"
                     /old_locus_tag="pgaptmp_001977"
     CDS             complement(7466..7786)
                     /gene="cyaY"
                     /locus_tag="ACHOSC_RS09885"
                     /old_locus_tag="pgaptmp_001977"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000999950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron donor protein CyaY"
                     /protein_id="HGU4547919.1"
                     /translation="MNDSEFHRLADQLWLTIEERLDDWDGDSDIDCEINGGVLTITFE
                     NGSKIIINRQEPLHQVWLATKQGGYHFDLKGDEWICDRSGETFWDLLEQAATQQAGET
                     VSFR"
     gene            complement(7834..9159)
                     /locus_tag="ACHOSC_RS09890"
                     /old_locus_tag="pgaptmp_001978"
     CDS             complement(7834..9159)
                     /locus_tag="ACHOSC_RS09890"
                     /old_locus_tag="pgaptmp_001978"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001584058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DKNYY family protein"
                     /protein_id="HGU4547920.1"
                     /translation="MKGQIITVALILLGHTFLTPAVQAIGFDYYNDHGVMSYGKGYWG
                     DKKIIAQFPEMSRADLRLVTNISGERELVEGYIPTDEISIKNEEYHWVTDGRVILWRG
                     KIVSNPPGTPTVDIASFQAMGRFAVDKYSLYFDGQRTESNSGASRVDLATLKAIEGNS
                     TTLVDSKNLYLSGRRQGSSSNVTVLEKRWWGINPRLMSVNRNLYSNDLLIRSGQNIYL
                     NGVHLTANADSFEIIRWIPHSLLVFRDNKGMHRYPFGQLSGKAIPVDDDVSFEVGESR
                     VRWRKQLTSDRQWSKWIDLPDIEPEQFHLITGNIAQYKDRLYVTKLSTFGEDQLEIIP
                     LDTPDLVIDRSFNSGKQHAYFIRQLRSKSVQIIPVNGPLTKNDRFAYDDRNVYTWTDT
                     EVRITPSPCPAKTHVREENVRELHNRDIIIPLTDDSCRNAATDGQTLKP"
     gene            complement(9156..10664)
                     /locus_tag="ACHOSC_RS09895"
                     /old_locus_tag="pgaptmp_001979"
     CDS             complement(9156..10664)
                     /locus_tag="ACHOSC_RS09895"
                     /old_locus_tag="pgaptmp_001979"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000580503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DKNYY family protein"
                     /protein_id="HGU4547921.1"
                     /translation="MIGTSRQDGFMHYRNVVCLLSCSLFLSSAWGCRLDPPEHNIYQK
                     QGKGVVYLRPYKETNLSLPDVNYKSLRRLPNLLIDPTTLDKWDKVPPLTDLTTDYLYE
                     GAQAWYPHYSWHSDGRYILYAGEVVQNPPDKPPVDVASFKAWGDFAADKHSLYFEGKR
                     TDNNGGGNRLDIKTLHQVEFRPPWDPDLLGLILRDANFLYINGHRLADPDSFRVLAQK
                     SWDQRGKFSTAFNPCVAMPFGPWDTLARTRTKILINGEQLDADPDTFSVVRWMPGSLL
                     TWRDKNGLQRKVLDKENLEWDEDLTKHCLDFSLLEKKVFWRKGPACKQEELPGLDPEQ
                     FQPISDAVAQYQDSLYTIIETESGDRKLEIVKLDDPNLIINKRFNAGKRHGYLLTRAE
                     GWPYHSGLHVFESDGPLILLDNRSPDEREAHLNDHPFLRRWYARDNRYIYSFDGAQLW
                     RYRTADPKQVRLIWKEQHSGYGYGVNYKTGYLDGKITDDGEFIPAPRNEATK"
     gene            complement(11002..11556)
                     /locus_tag="ACHOSC_RS09900"
                     /old_locus_tag="pgaptmp_001980"
     CDS             complement(11002..11556)
                     /locus_tag="ACHOSC_RS09900"
                     /old_locus_tag="pgaptmp_001980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000522548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3304 domain-containing protein"
                     /protein_id="HGU4547922.1"
                     /translation="MGLFTQLEKFDQKLTRGYARWGRWVWRTLIVVPVLVVLWNIGQA
                     VWGGPRGGVILEIHSEIDRPILGFSVNGVAGANAFANGGGSTTCCGDVSGDTAEVIWT
                     LSTTRTQYNAGMRLEKRNMTLPLPKREWGEDFLHVHFMPGDKVLLGWSKDSFSPYEDL
                     HNGGYKTRVRQDVKDKLYGTGKMN"
     gene            11906..12109
                     /gene="yifL"
                     /locus_tag="ACHOSC_RS09905"
                     /old_locus_tag="pgaptmp_001981"
     CDS             11906..12109
                     /gene="yifL"
                     /locus_tag="ACHOSC_RS09905"
                     /old_locus_tag="pgaptmp_001981"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HGU4547923.1"
                     /translation="MKNVFKALTVLITLFSLTGCGLKGPLYFPPADKNAPPPTKPVET
                     QTQSTVPDKNDRATGDGPSQVNY"
     gene            12146..12970
                     /gene="dapF"
                     /locus_tag="ACHOSC_RS09910"
                     /old_locus_tag="pgaptmp_001982"
     CDS             12146..12970
                     /gene="dapF"
                     /locus_tag="ACHOSC_RS09910"
                     /old_locus_tag="pgaptmp_001982"
                     /EC_number="5.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005133314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008837 - diaminopimelate epimerase
                     activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate epimerase"
                     /protein_id="HGU4547924.1"
                     /translation="MQFSKMHGLGNDFMVVDAVTQNVFFSPELIRRLADRHLGVGFDQ
                     LLVVEPPYDPELDFHYRIFNADGSEVAQCGNGARCFARFVRLKGLTNKRDIRVSTANG
                     RMVLTVTDDDLVRVNMGEPNFEPSAVPFRANKAEKTYIMRAAEQTILCGVVSMGNPHC
                     VIQVDDVDTAAVETLGPVLESHERFPERANIGFMQVVKREHIRLRVYERGAGETQACG
                     SGACAAVAVGIQQGLLAEEVRVELPGGRLDIAWKGPGHPLYMTGPAVHVYDGFIHL"
     gene            12967..13674
                     /gene="yigA"
                     /locus_tag="ACHOSC_RS09915"
                     /old_locus_tag="pgaptmp_001983"
     CDS             12967..13674
                     /gene="yigA"
                     /locus_tag="ACHOSC_RS09915"
                     /old_locus_tag="pgaptmp_001983"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF484 family protein"
                     /protein_id="HGU4547925.1"
                     /translation="MKQPGEELQETLTELDDRAVVDYLIKNPEFFIRNARAVEAIRVP
                     HPVRGTVSLVEWHMARARNHIHVLEENMALLMEQAIANEGLFYRLLYLQRSLTAASSL
                     DDMLMRFHRWARDLGLAGASLRLFPDRWRLGAPSNHTHLALSRQSFEPLRIQRLGQEQ
                     HYLGPLNGPELLVVLPEAKAVGSVAMSMLGSDADLGVVLFTSRDASHYQQGQGTQLLH
                     EIALMLPELLERWIERV"
     gene            13671..14567
                     /gene="xerC"
                     /locus_tag="ACHOSC_RS09920"
                     /old_locus_tag="pgaptmp_001984"
     CDS             13671..14567
                     /gene="xerC"
                     /locus_tag="ACHOSC_RS09920"
                     /old_locus_tag="pgaptmp_001984"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine recombinase XerC"
                     /protein_id="HGU4547926.1"
                     /translation="MTDLHTDVERYLRYLSVERQLSPITLLNYQRQLEAIINFASENG
                     LQSWQQCDVTMVRNFAVRSRRKGLGAASLALRLSALRSFFDWLVSQNELKANPAKGVS
                     APKAPRHLPKNIDVDDMNRLLDIDINDPLAVRDRAMLEVMYGAGLRLSELVGLDIKHL
                     DLESGEVWVMGKGSKERRLPIGRNAVAWIEHWLDLRDLFGSEDDALFLSKLGKRISAR
                     NVQKRFAEWGIKQGLNNHVHPHKLRHSFATHMLESSGDLRGVQELLGHANLSTTQIYT
                     HLDFQHLASVYDAAHPRAKRGK"
     gene            14567..15283
                     /gene="yigB"
                     /locus_tag="ACHOSC_RS09925"
                     /old_locus_tag="pgaptmp_001985"
     CDS             14567..15283
                     /gene="yigB"
                     /locus_tag="ACHOSC_RS09925"
                     /old_locus_tag="pgaptmp_001985"
                     /EC_number="3.1.3.104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-amino-6-(5-phospho-D-ribitylamino)uracil
                     phosphatase YigB"
                     /protein_id="HGU4547927.1"
                     /translation="MRFYRPLGRISALTFDLDDTLYDNRPVILRTEREALTFVQNYHP
                     ALRSFQNEDLQRLRQAVREVEPEIYHDVTRWRFRSIEQAMLDAGLSAEEASAGAHAAM
                     INFAKWRSRIDVPQQTHDTLKQLAKKWPLVAITNGNAQPELFGLGDYFEFVLRAGPHG
                     RSKPFSDMYFLAAEKLNVPIGEILHVGDDLTTDVGGAIRSGMQACWIRPENGDLMQTW
                     DSRLLPHLEISRLASLTSLI"
     gene            15367..17529
                     /gene="uvrD"
                     /locus_tag="ACHOSC_RS09930"
                     /old_locus_tag="pgaptmp_001986"
                     /gene_synonym="mutU"
                     /gene_synonym="recL"
     CDS             15367..17529
                     /gene="uvrD"
                     /locus_tag="ACHOSC_RS09930"
                     /old_locus_tag="pgaptmp_001986"
                     /gene_synonym="mutU"
                     /gene_synonym="recL"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0006268 - DNA unwinding involved in DNA
                     replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase II"
                     /protein_id="HGU4547928.1"
                     /translation="MDVSYLLDSLNDKQREAVAAPRSNLLVLAGAGSGKTRVLVHRIA
                     WLMSVENCSPYSIMAVTFTNKAAAEMRHRIGQLMGTSQGGMWVGTFHGLAHRLLRAHH
                     MDANLPQDFQILDSEDQLRLLKRLIKAMNLDEKQWPPRQAMWYINSQKDEGLRPHHIQ
                     SYGNPVEQTWQKVYQAYQEACDRAGLVDFAELLLRAHELWLNKPHILQHYRERFTNIL
                     VDEFQDTNNIQYAWIRLLAGDTGKVMIVGDDDQSIYGWRGAQVENIQRFLNDFPGAET
                     IRLEQNYRSTSNILSAANALIENNNGRLGKKLWTDGADGEPISLYCAFNELDEARFVV
                     NRIKTWQDNGGALAECAILYRSNAQSRVLEEALLQASMPYRIYGGMRFFERQEIKDAL
                     SYLRLIANRNDDAAFERVVNTPTRGIGDRTLDVVRQTSRDRQLTLWQACRELLQEKAL
                     AGRAASALQRFMELIDALAQETADMPLHVQTDRVIKDSGLRTMYEQEKGEKGQTRIEN
                     LEELVTATRQFSYNEEDEDLMPLQAFLSHAALEAGEGQADTWQDAVQLMTMHSAKGLE
                     FPQVFIVGMEEGMFPSQMSLDEGGRLEEERRLAYVGVTRAMQKLTLTYAETRRLYGKE
                     VYHRPSRFIGELPEECVEEVRLRATVSRPVSHQRMGTPMVENDSGYKLGQRVRHAKFG
                     EGTIVNMEGSGEHSRLQVAFQGQGIKWLVAAYARLESV"
     gene            complement(17676..18440)
                     /gene="yigE"
                     /locus_tag="ACHOSC_RS09935"
                     /old_locus_tag="pgaptmp_001987"
     CDS             complement(17676..18440)
                     /gene="yigE"
                     /locus_tag="ACHOSC_RS09935"
                     /old_locus_tag="pgaptmp_001987"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphodiester glycosidase family protein"
                     /protein_id="HGU4547929.1"
                     /translation="MAHRLLIGKGMITLNLKRIFLTLTLLPLFAVAADDCALSDPTLT
                     VQAYTVNPQTERVKMYWQKANGEAWGTLHALLADMNSQGQVQMAMNGGIYDESYAPLG
                     LYIENGQQKVALNLASGEGNFFIRPGGVFYVAGDKVGIVRLDAFKTSKEIQFAVQSGP
                     MLMENSVINPRIHPNVASRKIRNGVGINKHGNAVFLLSQQATNFYDFACYAKAKLNVE
                     QLLYLDGTISHMYMKGGAIPWQRYPFVTMISVERKG"
     gene            18602..18658
                     /gene="ysgD"
                     /locus_tag="ACHOSC_RS09940"
                     /old_locus_tag="pgaptmp_001988"
     CDS             18602..18658
                     /gene="ysgD"
                     /locus_tag="ACHOSC_RS09940"
                     /old_locus_tag="pgaptmp_001988"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YsgD"
                     /protein_id="HGU4547930.1"
                     /translation="MDTPSRYWLTILSSRINS"
     gene            18810..19760
                     /gene="corA"
                     /locus_tag="ACHOSC_RS09945"
                     /old_locus_tag="pgaptmp_001989"
     CDS             18810..19760
                     /gene="corA"
                     /locus_tag="ACHOSC_RS09945"
                     /old_locus_tag="pgaptmp_001989"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000947142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015087 - cobalt ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0015095 - magnesium ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006824 - cobalt ion transport [Evidence
                     IEA];
                     GO_process: GO:0015693 - magnesium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium/cobalt transporter CorA"
                     /protein_id="HGU4547931.1"
                     /translation="MLSAFQLENNRLTRLEVEESQPLVNAVWIDLVEPDDDERLRVQS
                     ELGQSLATRPELEDIEASARFFEDDDGLHIHSFFFFEDAEDHAGNSTVAFTIRDGRLF
                     TLRERELPAFRLYRMRARSQSMVDGNAYELLLDLFETKIEQLADEIENIYSDLEQLSR
                     VIMEGHQGDEYDEALSTLAELEDIGWKVRLCLMDTQRALNFLVRKARLPGGQLEQARE
                     ILRDIESLLPHNESLFQKVNFLMQAAMGFINIEQNRIIKIFSVVSVVFLPPTLVASSY
                     GMNFEFMPELKWSFGYPGAIIFMILAGLAPYLYFKRKNWL"
BASE COUNT         4609 a         5161 c         5193 g         4905 t
ORIGIN      
        1 aaatattcac tcaggaagtt attactcagg aagcaaagag gattacagaa ttatctcata
       61 acaagtgtta agggatgtta tttcccgatt ctctgtggca taataaacgg gtagatgctc
      121 attccatctc ttatgttcgc cttagtgcct cataaactcc ggaatgacgc agagccgttt
      181 acggtgctta tcgtccactg acagatgtcg cttatgcctc atcagacacc atggacacaa
      241 cgttgagtga agcacccact tgttgtcata cagacctgtt ttaacgcctg ctccgtaata
      301 agagcaggcg tttttttatg catcagaaag gtcccggagg tgcttgcctc cgggtgagaa
      361 ggaactactg tggtgggttg ttctgcaacg ttaacatcaa accgtcgcga cgcatcgctg
      421 cagcttcttc cggcttgtgc agtctgtcca gcgcgtcggc aagccatgcg taatcgtagg
      481 cgtccggacg ttgtttcagc gccgcgcgga aggcgagcga tgcttcctgc cattctccgt
      541 gcttcatcag tgactggccc agtgtgctcc acaacagcgg gcgatcgccg acgtttttga
      601 tctgctggcg cagcactttt tccagctgtt ccggattgtt ggttttcagt cgaggaatcg
      661 gcagcagtag gcgatcgtcg tactggcgtt tcaggccatc gatgataatt tgctgggcag
      721 tatcatgatc gtcacattca ataagatgtt ccgccattgc cacctgcaac gctacctgat
      781 gacgcgtttt ccggctttgg tttttccacc agttacgcaa accttcacta ccgttatcgg
      841 cacgcgcctg atccatcagg ccaatccatg cctgttgttc cagcattgca cgatgttctt
      901 catcaccaac atgggctttc gccattgatg ggatgatatc cagcagcgaa ctccatgcac
      961 ctgtgcggat atacgcctgt tccgccagac gtaatacttc cggatggcgt ggcgtaactt
     1021 ccagcagctt atccacgccg tggcgtgcag catggttttc attacgggcc agttgcagac
     1081 gtacacgggt gatttctacc ggaatggtgt cattgccggc cagctctgct gcgcgttcca
     1141 gatgttggtt ggcgcgtgct tcatcaccac gttgttgcgc ggcttcggca gccagtagat
     1201 agttcaccac cggttgttcc gcgtgatcgg catttttcgc catcagcttt tcaacttgct
     1261 gataatcgcc ttccgccagt ttcagcagcg cctgttcggt ctgcttacgg gcacggcgac
     1321 gcttacgtcc gacaaaccac ccacgggtgt gtgcgcctgt gcggaagatc cgccgcagta
     1381 gccactcaat ggcaaacagc actaccatcg ccagaatcaa aataatcgcc aggcccgtga
     1441 cgctggtttc gatattgtag ttgtcagtct ggatcagcac gtaaccctga tgaccggcaa
     1501 tcatcgggcc aaccactatc cccgcaatca gcaaaacaaa gagtaataac acttttagca
     1561 tggttattct ccttgcggcg cggctgccgg agtatcacct tgcggtgcag gtgcaggtgc
     1621 aggtgcaggt gcaggtgcag gtgcaggtgc aggtgcaggt gcaggtttag cttccgttgt
     1681 ccccgctgcc ggttgtgcca gcaggttacg cacgcgggtc tgcatcagtt tttccagcat
     1741 cgcctggctt tgcagggttt ccggaagatc catcgagata ttttgctggc ttaactggtc
     1801 cacctcgtcg aggaacgctt tggtggtggc atcatcagta tcgtagtaag ctcgtaccca
     1861 ggtggagacg ttctccagcg cctggcgata agtctcttcc tggtgacgcg gtacagcttg
     1921 tgctgcgacc agcaggcgag agcgaatatt ttcgcgcaga tagatatcct gatttggcgc
     1981 taacagcggt acggcggtgt catcacgacg gcgaatcgta atgaagttgt ccataaagtt
     2041 ctgccagctt ttttgcagat tgatacgcca ttcgctgatg gaactggaaa gctcttcacc
     2101 gtcggaatcc atcggcgaac catcgctgtc attatcggcc agacgcaggt tatctacctg
     2161 atttgaaagc tgattaagct taaggatgat gccgtcataa tccacctgcg aaactgcaga
     2221 aaggctggcg atatcatcgg taattgcccg acgaacggta atcagactcg ggtcattcat
     2281 atccgccagg ctggcatctg cacttttcag caacgctgca gcggtcgtga cgtcctgatc
     2341 gctccacagc ttccgtccgg cgagtttcac cagaaaatcg gcctgagcca gcagccaggt
     2401 tttagcatcg ctgccggaaa tggtggcgac cttttgttgg acttcatcca actgttttgc
     2461 cagcgtttct tgctgacgat tcgcctgctt aagctgtgca gcttgttgct taataatgcc
     2521 ttccagctcg gctttttggc tctcctgggc tttttgcaat gccgtcagtt ggttagccag
     2581 ggcatcgctg gtggcggtct gattgacggc ctgttgttta ccccagccat acaaaccgac
     2641 acccgccgcc agagcaatag cgatagccac cgcgctgaga atcaatgcag tattgttctt
     2701 actctttttt tctgttgcga caggttgtga cgtggtgtcc acggcctccc tggtctcttc
     2761 aaccacggcg gaggtttttt cttgttccgt cattatggct tcctgttatg agagttattg
     2821 taatgcccgt aaaagcgcat cgttgtcagc gttatcggcg accttaatgt cttgccagcc
     2881 cagttcccgg gcgagtttcg ccaaacgctc actgacgacc aatagtcgac agtgtagtaa
     2941 ccagtgctca cgataccatt gtgggatcag cgaccagagt tgctgcaaca tttcaccgct
     3001 ggtaacaacg accatcgtca cctcgcggga ttgccagcgc atcgcttctt ctgcaccatc
     3061 gtaatggatt gcgcatcgtt gataacattc acaaaaagtg acctcagcac cgcgcgctgt
     3121 cagggtatcc ccaattagct cacgaccgcc attgccacgt aatatcagcg cacgtttgcc
     3181 cgcaatattt tgtaattcag gtaattgtag caagacttcg ctgatttccc gatcctgcgg
     3241 gtagagaatc ttctgtccgc ttacggtatg tagcgccagt gcggtggtgc gtccaatggc
     3301 gaaataatca ggtagtcggg gccatttacg atcttgctga tgcagctgtg attgggcaaa
     3361 agcaaccgcg tgttgcgaga gggcaaacaa cagatcgctc tcccccagcg ccgccagttg
     3421 atcagcaagt tgcggtaatt gtcgacccgg agaaaactca atcagcggaa aatgccaggc
     3481 cacctgcccc agtgtgcgca gacggctcac taactcttct ccagcgggag acgggcgggt
     3541 gaccaggata ctcatgccgg ggcgtctccg ttatagactt cagcgaggat ctcgcgcgcg
     3601 ccgttattca gtagctcttc tgccagcgaa atccccattt gttcggcatt ttgcggcgca
     3661 ccgcggcgtt caccgcgaat aatctgcgaa ccgtccggcg cgccgaccag cgcacgcagc
     3721 cagatttcac catcaataag ctcggcgtag ctaccaattg gcacctgaca tccaccttcg
     3781 agacgggtat tcatggcgcg ttctgcggta acgcgcagtg cagtttcgtg gtgattcagc
     3841 gcggcaagca gctcgcgagt gcgcgtatca tcaaggcggc attcaatacc gactgcacct
     3901 tgtcctaccg ccggaagaga aatctcgggt ggtaacgcgg cgcgaatgcg ggactccaga
     3961 cctaaacgtt ttagtccggc tacggcaaga atgatggcat cgtattcgcc gttatctagt
     4021 ttgctcaggc gagtgccgac gttgccgcgc agggagcgga taatcagatc cgggcggcgt
     4081 tcagccagtt ggcactggcg acgtaaactg gacgtcccga cgatactgcc tgccggtaac
     4141 gcatccagac tgtcataatt attggacaca aaggcatcgc gaggatcttc acgctcacaa
     4201 atagtgacca accccagacc ttgcgggaat tcaaccggca catctttcat tgaatgtacg
     4261 gcgatatcgg cgcgattctc caggagcgcg acttccagct cttttacaaa taagcctttt
     4321 ccgcctactt tcgccagcgg cgtatcaaga atcacatcgc cgcgcgtcac catcggtacc
     4381 agttcaacca ccaggcccgg atggctcgcc atcaacttgt ctttgacata gtgtgcctgc
     4441 cagagtgcaa gtgggctttg gcgtgtggca attcttaaaa cattgtctaa catgcttgtt
     4501 accgtcatta tcatccgtgg tccatcctaa catccttgcc agagtgatgt cagtgttgtg
     4561 gtgaaacgta gacgcctgcg caaaccgtaa aatgaggtct ggcagtggat cctgacaggc
     4621 gtttcacgcc gtcgtaataa ggaatttaca gagaataaac ggtgctacac ttgtatgtag
     4681 cgcatctttc tttacggtca atcagcaagg tgttaaattg atcacgtttt agaccatttt
     4741 ttcgtcgtga aactaaaaaa accaggcgcg aaaagtggta acggttacct ttgacatacg
     4801 aaatatcccg aatgccgcgt gttaccgttg atgttggcgg aatcacagtc atgacgggta
     4861 gcaaatcagg cgatacgtct tgtacctcta tattgagact ctgaaacaga gactggatgc
     4921 cataaatcaa ttgcgtgtgg atcgcgcgct tgctgctatg gggcctgcat tccaacaggt
     4981 ctacagtcta ctgccgacat tgttgcacta tcaccatccg ctaatgccgg gttaccttga
     5041 tggtaacgtt cccaaaggca tttgccttta cacgcctgat gaaactcaac gccactacct
     5101 gaacgagctt gaactgtatc gtggaatgtc agtacaggac ccaccgaaag gtgagcttcc
     5161 aattactggt gtatacacca tgggcagcac ctcgtccgta gggcaaagtt gttcctctga
     5221 cctggatatc tgggtctgtc atcaatcctg gctcgatagc gaagagcgcc agttactaca
     5281 acgtaaatgt agtctgctgg aaagctgggc cgcctcgctg ggtgtggaag tcagcttctt
     5341 cctgattgat gaaaaccgct tccgtcataa tgaaagcggc agcctggggg gcgaagattg
     5401 tggctccacc cagcatatac tgctgcttga cgaattttat cgtaccgccg tgcgtctcgc
     5461 cggtaagcgt attctgtgga atatggtgcc gtgcgacgaa gaagagcatt acgacgacta
     5521 tgtgatgacg ctttacgcgc agggcgtgct gacgccaaat gaatggctgg atctcggtgg
     5581 cttaagctcg ctttctgctg aagagtactt tggtgccagc ctttggcagc tctacaagag
     5641 tatcgattcc ccatacaaag cggtactgaa aacactgctg ctggaagcct attcctggga
     5701 atacccgaac ccacgtctgc tggcgaaaga tatcaaacag cgtttgcacg acggcgagat
     5761 tgtatcgttt ggtctcgatc catactgcat gatgctggag cgtgttactg aatacctgac
     5821 ggcgattgaa gattttaccc gtctggattt agtacgtcgc tgcttctatt taaaagtgtg
     5881 cgaaaagctc agccgtgaac gcgcctgcgt aggctggcgt cgcgcagtgt tgagccagtt
     5941 agtgagcgag tggggttggg acgaagctcg tctggcaatg ctcgataacc gcgctaactg
     6001 gaagattgat caggtgcgtg aggcgcacaa cgagttgctc gacgcgatga tgcagagcta
     6061 ccgtaatctg atccgctttg cgcgtcgcaa taaccttagc gtctccgcca gtccgcagga
     6121 tatcggcgtg ctgacgcgta agctgtatgc cgcgtttgaa gcattaccag gtaaagtgac
     6181 gctggtaaac ccgcagattt cacccgatct ctcggaaccg aatctgacct ttatttatgt
     6241 gccgccgggc cgtgctaacc gttcaggttg gtatctgtat aaccgcgcgc caaatattga
     6301 gtcgatcatc agccatcagc cgctggaata taaccgttac ctgaacaaac tggtggcgtg
     6361 ggcatggttt aacggcctgc tgacctcgcg cacccgtttg tatattaaag gtaacggcat
     6421 tgtcgatttg cctaagttgc aggagatggt cgccgacgtg tcgcaccatt tcccgctgcg
     6481 cttacctgca ccgacaccga aggcgctcta cagcccgtgt gagatccgcc atctggcgat
     6541 tatcgttaac ctggaatatg acccgacagc ggcgttccgc aatcaggtgg tgcattttga
     6601 tttccgtaag ctggatgtct tcagctttgg cgagaatcaa aattgcctgg taggtagcgt
     6661 tgacctgctg taccgcaact cgtggaacga agtgcgtacg ctgcacttca acggcgagca
     6721 atcgatgatc gaagccctga aaactattct cggcaaaatg catcaggacg ccgcaccgcc
     6781 agatagcgtg gaagtcttct gttatagcca gcatctgcgc gggttaatcc gtactcgcgt
     6841 gcagcaactg gtttctgagt gtattgaact gcgcctttcc agcacccgcc aggaaaccgg
     6901 gcgtttcaag gcgctgcgcg tttctggtca aacctggggg ttgttcttcg aacgcctgaa
     6961 tgtatcggta caaaaactgg aaaacgccat cgagttttat ggcgctattt cgcataacaa
     7021 actgcacggc ttgtcagtgc aggttgaaac caatcacgtc aaattaccgg cggtggtgga
     7081 cggctttgcc agcgaaggga tcatccagtt cttcttcgaa gaaacgcaag acgagaatgg
     7141 ctttaatatc tacattctcg acgaaagcaa ccgggttgag gtgtatcacc actgcgaagg
     7201 cagcaaagag gagctggtac gtgacgtcag tcgcttctac tcgtcatcgc acgatcgctt
     7261 cacctacggc tcaagcttta tcaacttcaa cctgccgcag ttctatcaga ttgtgaaggt
     7321 tgatggtcgt gaacaggtga ttccgttccg cacaaaatct atcggtaaca tgccgcccgc
     7381 caatcaggac aacgatacgc cgctattaca gcagtatttt tcgtgatgag cgtgccggaa
     7441 agcgaggctt atccggcatg caatcttagc ggaaactgac tgtttcaccc gcttgctgtg
     7501 tcgccgcctg ttccagcaaa tcccagaagg tttcgccgct gcgatcgcaa atccattcat
     7561 cgccttttag gtcaaaatgg tagccgccct gtttggttgc cagccacacc tggtgcagcg
     7621 gctcctggcg gttaataatg attttgctgc cgttttcaaa ggtaatggtc agtacgccgc
     7681 cgttgatttc gcagtcgata tcgctgtcgc catcccagtc gtccaggcgt tcttcaatgg
     7741 tcagccagag ttgatcagcc aggcgatgaa attcactgtc gttcattgtt gtatcctgtc
     7801 tttaagtgat ggcggcagta tagcggtatg gggtcagggc ttcaaagttt gcccgtctgt
     7861 tgctgcgttc cggcatgagt catccgtcaa cggaataata atgtctctgt tgtgaagttc
     7921 acgcacattc tcttctctga cgtgtgtttt cgccgggcag ggggagggcg taatccttac
     7981 ctctgtatcg gtccatgtat aaacattgcg atcgtcataa gcgaagcgat cgtttttagt
     8041 tagcggaccg ttaactggaa taatttgcac gctctttgac cgtaattggc ggataaaata
     8101 agcatgctgt ttgccgctat taaatgagcg atcaatgacc aggtctggcg tatccagcgg
     8161 gattatctca agctggtctt caccaaatgt cgataatttt gttacataca gccgatcttt
     8221 atattgcgca atattgccag taatcagatg gaattgttca ggttcaatat ctggtaggtc
     8281 tatccacttg ctccactgac ggtcggacgt gagctgttta cgccagcgaa cgcgactttc
     8341 ccctacttca aaagagacgt catcatctac gggtatcgct ttgccagaca attgcccaaa
     8401 gggataacga tgcataccct tattgtcgcg aaaaaccagc agtgaatgag gtatccagcg
     8461 aattatctca aatgagtctg cattcgccgt aaggtgaacg ccatttaaat aaatattctg
     8521 cccactgcgg ataagcagat cattggaata caaatttctg tttacactca taagacgtgg
     8581 attaataccc caccatcttt tttctaatac agtaacatta ctgctgctcc cctgacgtcg
     8641 accggacaaa tagagatttt tactatccac cagcgtggta gagttacctt cgatagcttt
     8701 tagtgtcgcc agatcaacac gtgacgcgcc gctattactt tcagtgcgct gtccgtcaaa
     8761 atagaggcta tatttatcga ccgcgaagcg gcccatagcc tgaaagctgg caatatcgac
     8821 agtgggggtt cccggtggat tgctaacgat tttgccacgc cagagaataa cgcggccgtc
     8881 tgtcacccag tggtattcct cattttttat gcttatttca tcggtgggta tatagccttc
     8941 caccaactcc cgctccccgg atatatttgt cacaaggcgc aaatcggccc tgctcatctc
     9001 cggaaactgg gcaatgattt tcttatcccc ccaataacct ttaccgtaag acataacacc
     9061 gtgatcattg taatagtcga accctatggc ctgtacagca ggcgtcagaa atgtgtgtcc
     9121 caacaggatg agtgcaactg tgattatttg acccttcatt ttgtcgcctc attgcgcggg
     9181 gcaggaataa attcgccgtc atcagtaatt tttccgtcca gatatcccgt tttgtaattt
     9241 acgccatagc catatcccga atgttgttcc ttccagatta agcgaacttg tttcggatca
     9301 gcggtgcggt atcgccagag ctgcgcgcca tcaaagctgt aaatgtagcg gttatcgcgg
     9361 gcataccatc tgcgcaaaaa gggatggtca ttaagatggg cttcgcgttc atccggagaa
     9421 cggttatcca gtaagatcag cggtccgtca gattcaaaca catgtaaacc ggaatggtat
     9481 ggccaccctt cggcacgcgt aagtaaatag ccgtggcgtt tcccggcgtt gaaacgtttg
     9541 ttgataataa gattgggatc atcaagtttc acgatttcca gcttgcggtc accagactct
     9601 gtttcgatga tggtataaag cgagtcctga tactgggcga cagcatcact gatgggttga
     9661 aactgctccg gatcgagtcc gggtaattct tcctgtttac aagcaggtcc tttacgccag
     9721 aacactttct tttccagcag agaaaaatcc agacagtgct ttgttaaatc ttcatcccac
     9781 tccagatttt ccttgtcgag gactttacgc tgcagcccgt ttttatcacg ccaggtcaag
     9841 agtgagccgg gcatccagcg cacgacggaa aaggtgtccg ggtcggcatc aagctgttcg
     9901 ccgttgatca ggattttggt ccgtgtacga gccagggtat cccaggggcc aaatggcata
     9961 gccacacagg gattaaacgc ggtagaaaac tttcctcgct gatcccatga cttttgtgcc
    10021 agcacacgga aactatcggg atctgcgagg cgatgcccat tgatatacaa gaaattagcg
    10081 tcacgtaata tcagccccag taagtcagga tcccagggag gccggaattc cacctgatgc
    10141 agtgttttga tatctaagcg atttcccccg ccattgttat cggtgcgctt gccttcgaaa
    10201 tagagactgt gtttatctgc ggcaaaatca ccccacgcct tgaacgaggc gacatcgacg
    10261 ggaggtttgt ctggcgggtt ttgcacaact tcacccgcgt agaggatata gcgaccatcg
    10321 ctatgccagg agtaatgagg ataccaggcc tgtgcgcctt catagaggta gtcggttgtc
    10381 agatcagtca gaggaggtac tttatcccat ttgtctagtg ttgtcgggtc tattaataaa
    10441 ttgggtaaac ggcgcaaact tttgtaattc acgtcaggaa gagaaagatt tgtttcttta
    10501 taagggcgca ggtataccac gccttttccc tgtttctggt aaatattatg ttcaggcgga
    10561 tcaagccgac atccccatgc cgacgataaa aacaatgaac aagacaacaa acaaaccaca
    10621 ttgcgatagt gcataaagcc atcctggcgc gaggtgccga tcacgaaact accagcagaa
    10681 cataattcct cgcacgtaag cgtaactttt gcggcaaatc gtcacttttc agaatctata
    10741 aaggccatcg ctattttctg atagttgctg gggtaaactt gaggttttca ttcacgatat
    10801 aaatacgagc tactgcgcca tggtgtgact gatcacgtta aaatgtgccg gattgacgcc
    10861 gggaagggtg tcaacatgcg actctgccca ccgttaccta ctttattgtc tttcgcgtgt
    10921 tgtacttagg tcccaccacc ggtatctaac attccccaca ttattacgct ttctgccgaa
    10981 cgtgattttt atagaaatct atcaattcat tttccctgta ccataaagtt tatctttcac
    11041 atcctgccga acgcgggtct tgtacccccc gttgtgaaga tcttcatacg gagaaaaaga
    11101 atctttactc caccccagca gcactttatc gccaggcata aaatgcacat gcagaaaatc
    11161 ttctccccat tctcttttgg ggagaggtaa cgtcatattc cgtttctcca gacgcattcc
    11221 agcattatac tgtgtacgag tagtacttaa tgtccagata acctctgccg tatccccact
    11281 aacatcacca caacaggttg ttgaaccgcc accatttgca aaagcattcg cgcccgccac
    11341 gccgttaaca ctgaatccca gtatcgggcg gtcaatttca ctgtggattt ccagaatcac
    11401 tccacctctt ggtcctcccc atactgcctg tcctatattc cacaacacga ccagtaccgg
    11461 aacgacaatt aacgtccgcc atacccagcg cccccaccgg gcataaccgc gcgtaagctt
    11521 ttggtcaaat ttttccagtt gggtaaatag ccccatcaga ttttattccc tcttatcacc
    11581 aggttggcaa agttgtatgt cactggcgac taaggtcaga caacaaaatt gcatggtgcg
    11641 ccactctttt aaaagctcca tgccgacgat aagaacaacg aacaagccaa caaacaaact
    11701 acattgcgat agtgcataga gccatcctgg cgcgaggtgc cgatcacgaa actaccagca
    11761 aaacataaat ccccacgagt aagcgttata ctcgcagcat ttcctcactt ttcagacttc
    11821 ataaagagtc gctaaacgct tgcttttacg tcttctcctg cgatgataga aagcagaaag
    11881 cgatgaactt tacaggcaat ccataatgaa aaacgtgttt aaggcactca ctgtattaat
    11941 tactctcttc agcctgacgg gctgcggtct gaaaggtccg ctctatttcc cgcctgcaga
    12001 taaaaacgca ccgccgccga ccaaaccggt agagacgcaa acgcaatcca cggtgccgga
    12061 taaaaacgat cgcgccactg gcgatggtcc atcccaggtg aattactaaa agtcagtttc
    12121 tgtacccgcg tgattggagt aaatgatgca gttctcgaaa atgcatggcc ttggcaacga
    12181 ttttatggtc gtcgacgcgg taacgcagaa tgtctttttt tcaccggagc tgattcgtcg
    12241 cctggctgat cggcacctgg gggtagggtt tgaccaactg ctggtggttg agccgccgta
    12301 tgatcctgaa ctggattttc actatcgcat tttcaatgct gatggcagtg aagtggcgca
    12361 gtgcggcaac ggtgcgcgct gctttgcccg ttttgtgcgt ctgaaaggac tgaccaataa
    12421 gcgtgatatc cgcgtcagca ccgccaacgg gcggatggtt ctgaccgtca ccgatgatga
    12481 tctggtccgc gtaaatatgg gcgaacccaa cttcgaacct tccgccgtgc cgtttcgcgc
    12541 taacaaagcg gaaaagacct atattatgcg cgccgccgag cagacaatct tatgcggcgt
    12601 ggtgtcgatg ggaaatccgc attgcgtgat tcaggtcgat gatgtcgata ccgcggcggt
    12661 agaaacgctt ggtcctgttc tggaaagcca cgagcgtttt ccggagcgcg ccaatatcgg
    12721 ttttatgcaa gtggttaagc gcgagcatat tcgtttacgc gtttatgagc gtggggcagg
    12781 agaaacccag gcctgcggca gcggcgcgtg tgcggcggtt gcagtaggga ttcagcaagg
    12841 tttgctggcc gaagaagtac gcgtggaact ccccggcggt cgtcttgata tcgcctggaa
    12901 aggtccgggt cacccgttat atatgactgg cccggcggta catgtctacg acggatttat
    12961 tcatctatga agcaaccagg ggaagaactg caggaaacac tcacggagct tgatgaccgg
    13021 gcggttgtcg attatctgat taaaaatcct gagtttttta tccgtaatgc gcgcgcagta
    13081 gaagcgatac gtgtgccgca tccggtacgc ggcaccgttt cgttggtcga gtggcacatg
    13141 gcccgcgcac gtaatcatat tcatgttctg gaagagaaca tggcgctgtt gatggaacag
    13201 gctatcgcca acgaaggcct gttttatcgc ctactctacc tgcagcgcag tctcaccgcc
    13261 gccagcagtc tcgacgatat gctgatgcgc tttcaccgct gggcgcgcga tctcggcctg
    13321 gcaggtgcga gtctgcgcct gtttccggat cgctggcgct taggtgcgcc gtcgaaccac
    13381 actcatctgg cattaagccg tcagtctttc gaaccgctgc gtattcagcg tttggggcag
    13441 gaacagcact atcttgggcc gcttaacgga ccagagctgc tggtggtgct accggaagcg
    13501 aaagcggtgg gatcggtggc gatgtcgatg ctgggaagcg atgctgattt gggtgtcgtg
    13561 ctgtttacca gtcgcgatgc cagtcactat caacaagggc aaggaacgca gttacttcat
    13621 gaaattgcgc tgatgttgcc ggagcttctg gagcgttgga ttgaacgcgt atgaccgatt
    13681 tacacaccga tgtagaacgc tacctacgtt atctgagcgt ggagcgccag cttagcccga
    13741 taaccctgct taactaccag cgtcagcttg aggcgatcat caattttgcc agcgaaaacg
    13801 gcctgcaaag ctggcagcaa tgtgatgtga cgatggtgcg caattttgct gtacgcagtc
    13861 gccgtaaagg gctgggagca gcaagtctgg cgttacggct ttctgcgcta cgtagctttt
    13921 ttgactggct ggtcagccag aacgaactca aagctaaccc ggcgaaaggt gtttcggcac
    13981 cgaaagcgcc gcgtcatctg ccgaaaaaca tcgacgtcga cgatatgaat cggctgctgg
    14041 atattgatat caacgatccc ctcgctgtac gcgaccgtgc aatgctggaa gtgatgtacg
    14101 gcgcgggtct gcgtctttct gagctggtgg ggctggatat taaacacctc gacctggagt
    14161 ctggtgaagt gtgggttatg gggaaaggca gcaaagagcg ccgcctgccg attggtcgca
    14221 acgctgtggc gtggattgag cactggcttg atttgcgcga cctgtttggt agcgaagacg
    14281 acgcgctttt tctgtcgaaa ctgggcaagc gtatctccgc gcgtaatgtg cagaaacgct
    14341 ttgccgaatg gggcataaaa caagggctga ataatcacgt tcacccacat aaattacgtc
    14401 actcgttcgc tacgcatatg ctggagtcga gcggcgatct tcgtggtgtg caggagctac
    14461 tgggtcatgc caacctctcc accacgcaaa tctatactca tcttgatttt caacaccttg
    14521 cctcggtgta cgatgcggcg catccacgcg ccaaacgggg gaaataatgc gtttttaccg
    14581 gcctttgggg cgcatctcgg cgctcacctt tgacctggat gatacccttt acgataaccg
    14641 tccggtgatt ttgcgcaccg agcgagaggc gcttaccttt gtgcaaaatt atcatccggc
    14701 gctgcgcagc ttccagaatg aagatctgca acgcctgcgc caggcggtac gggaagtgga
    14761 acccgagatt tatcacgacg tgacgcgctg gcgttttcgt tcgattgaac aagcgatgct
    14821 cgacgccggg ctgagtgccg aagaagccag tgcaggcgca cacgcagcaa tgatcaactt
    14881 tgccaaatgg cgcagccgaa tcgacgtccc gcagcaaact cacgacacct taaaacagct
    14941 ggcgaagaaa tggccgctgg tggcgatcac caacggtaac gcccagccgg aactgtttgg
    15001 tttgggggat tattttgagt ttgtgctgcg cgctggcccg cacgggcgct caaaaccgtt
    15061 cagcgatatg tactttctgg ctgcggaaaa actcaacgtg ccgatcggcg agatcttaca
    15121 tgttggggac gatctcacca ctgacgtggg tggggcaatt cgcagcggaa tgcaggcctg
    15181 ttggatcaga ccggaaaatg gcgatctgat gcaaacctgg gacagccgtt tactgccgca
    15241 tctggaaatt tcccggttgg catctctgac ctcgctgata taatcagcaa atctgtatat
    15301 atacccagct ttttggcgga gggcgttgcg cttctccgcc caacctattt ttacgcggcg
    15361 gtgccaatgg acgtttctta cctgctcgac agccttaatg acaaacagcg cgaagcggtg
    15421 gccgcgccac gcagcaacct tctggtgctg gcgggcgcgg gcagtggtaa gacgcgcgta
    15481 ctggtgcatc gtatcgcctg gctgatgagc gtggaaaact gctcgccata ctcgattatg
    15541 gcggtgacgt ttaccaacaa agcggcggcg gagatgcgtc atcgtatcgg gcagctgatg
    15601 ggcaccagcc agggcggcat gtgggtcggc accttccacg ggctggcgca ccgtctgctg
    15661 cgtgcgcacc atatggacgc caatctgccg caggattttc agatcctcga cagcgaggac
    15721 cagctacgcc tgctcaaacg cctaatcaag gcgatgaacc tcgacgagaa gcagtggccg
    15781 ccgcgccagg caatgtggta catcaacagc cagaaagatg aaggcctgcg tccacatcat
    15841 attcaaagct acggtaatcc ggtggagcag acctggcaga aggtgtatca ggcgtatcag
    15901 gaagcgtgtg accgcgcggg cctggtggac ttcgccgagc tgctgctacg tgctcacgag
    15961 ttgtggctta acaagccgca tatccttcag cactatcgcg aacggtttac caatatcctg
    16021 gtggacgaat tccaggatac caacaacatt cagtatgcgt ggatccgcct gctggcgggc
    16081 gataccggca aagtgatgat cgttggcgat gacgatcagt caatctacgg ctggcgtgga
    16141 gcgcaggtgg agaatattca gcgtttcctc aatgatttcc ccggtgctga aactatccgt
    16201 ctggagcaga actaccgctc taccagcaat attctgagcg ccgcgaacgc cctgattgaa
    16261 aacaacaacg ggcgtttggg taaaaaactg tggaccgatg gcgcggacgg tgagcctatt
    16321 tccctctatt gcgcttttaa cgaactcgat gaagcgcgtt ttgtggttaa ccgcatcaaa
    16381 acctggcagg acaacggcgg ggcgcttgcc gagtgcgcca ttctctaccg cagtaacgcc
    16441 cagtcgcgtg tgctggaaga agcactgttg caggcgagta tgccgtaccg tatttacggc
    16501 gggatgcgct tcttcgaacg ccaggaaatc aaagatgcgc tctcgtatct gcgcctgatt
    16561 gccaaccgta acgacgacgc ggcctttgag cgtgtggtga atacgccaac gcggggtatt
    16621 ggtgaccgga cgctggacgt ggtacgtcag acatcgcgcg atcgccagtt aacactctgg
    16681 caggcatgtc gtgaactgtt gcaggaaaaa gccctcgccg gacgtgctgc cagcgcctta
    16741 cagcggttta tggaactgat cgacgcctta gcgcaggaaa ctgccgatat gccgctgcat
    16801 gtacagactg accgggtaat taaagactcc ggcctgcgta ccatgtacga gcaggagaag
    16861 ggcgaaaaag gtcagacgcg tatcgaaaac ttagaggaac tggtgacggc aacgcgccag
    16921 ttcagctaca acgaagaaga cgaagattta atgccgctgc aggcgttcct atcccatgcg
    16981 gcgctggaag caggcgaagg gcaggcggat acctggcaag atgcggtaca actgatgacc
    17041 atgcactcgg cgaaaggtct ggagttcccg caggtgttta tcgtcggtat ggaagagggc
    17101 atgttcccaa gccagatgtc gctggatgaa ggcgggcgtc tggaagaaga acgccgtctg
    17161 gcctacgttg gcgtaacccg cgcgatgcag aaactgacgc tgacctacgc ggaaactcgc
    17221 cgtctgtatg gcaaagaggt ttaccatcgc ccgtcgcgct ttattggcga gctgccggaa
    17281 gagtgtgtgg aagaggtgcg cctgcgcgcc acggtaagcc gcccggtcag ccatcagcgg
    17341 atgggtacgc cgatggtcga gaacgacagc ggctacaagc tcggccagcg cgtacgccac
    17401 gctaagtttg gtgaaggcac cattgtcaat atggaaggca gcggtgagca tagccgtttg
    17461 caggtggcat ttcagggcca gggtattaaa tggctggtgg cggcttatgc ccggctggag
    17521 tcggtgtaac gttgccggat gcggtgctgc gcaccttact tggcctaaaa aatcattcag
    17581 attcaataaa ttgcaacgtc atgtaggccg gatagggcgt ttacgccgca tccggcatct
    17641 gcgccatctt cagtatctga cacaaaacta tcgttttaac ctttccgctc tacggaaatc
    17701 atggtgacaa aaggatagcg ttgccacggg attgccccgc ctttcatata catatgtgaa
    17761 atcgtgccat caagataaag cagctgctca acgttcagtt tcgctttggc ataacaggca
    17821 aaatcataaa aatttgtcgc ctgctggctc aacaaaaaca cggcattccc atgtttatta
    17881 atcccaacac cgttacgaat tttacgtgag gcgacgttgg gatgaatacg cggattaatt
    17941 acactgtttt ccatcaacat tggccctgac tgcaccgcaa actgaatctc tttactggtt
    18001 ttgaaggcat ccagacgaac gatgccgact ttatctcccg cgacataaaa cacgccgcca
    18061 ggacggataa agaaattccc ttcacctgaa gcgagattta acgccacctt ctgctgaccg
    18121 ttttcgatgt acaaaccgag cggcgcatag ctttcatcat agatgccgcc gttcatcgcc
    18181 atctgcacct gaccctgact attcatatcc gccagcagag catgtaaagt tccccacgct
    18241 tcgccattgg ctttttgcca gtacattttc acccgctctg tttgaggatt aacggtatac
    18301 gcctgtacgg tcagcgtcgg atctgagagt gcgcaatcat cagcggcaac agcaaacaag
    18361 ggaagtaagg tgagggtgag aaaaatccgt ttgagattca aggtgatcat tcctttacca
    18421 atgagtagcc gatgcgccat tataggtcct ggatgttgga tttttttatc ctgttagcga
    18481 ccttgacgag taccaaaaag cgcgaagttc aactattgtt ctgtggtgtt ctgttgcgtg
    18541 ttgacggcaa aattttgctg gcgtaacatg cgcgcacgat cactctaaga ggacattcgc
    18601 cttggacaca cccagtagat actggctcac tatcctgtca tccaggatca actcctaagg
    18661 ctatcccttt ttgctgatag ccttagcggt tgtcagcgac ctcaattttt cccgtcgcgc
    18721 tgagtcaggc tgtttaatgg tctgaaaccc aatttgtttc tgtgtgccca ccgaactgtc
    18781 cgatatttta agcattggga gtcccggtca tgctgagcgc atttcaactg gaaaataacc
    18841 gactgacccg gctggaagtc gaagagtcac aaccccttgt aaatgcagta tggattgatc
    18901 ttgtcgaacc ggacgacgac gagcgactgc gcgtacaatc tgaacttggc cagagcctgg
    18961 caacccgccc ggaactggaa gacatcgaag catcggcacg tttctttgaa gacgacgacg
    19021 gcctgcatat tcactccttc ttcttctttg aagatgcgga agatcacgcc ggtaactcca
    19081 ctgtggcatt taccatccgt gatggtcgtc tgtttactct gcgtgagcgt gaactgcccg
    19141 cttttcgtct gtatcgtatg cgtgcccgta gccagtcgat ggtagacggt aacgcctacg
    19201 agttgctgct ggatctgttc gaaaccaaaa tcgaacagtt ggcagatgaa attgaaaata
    19261 tctatagcga cctggagcag ttgagccggg tgattatgga agggcatcag ggcgatgagt
    19321 acgacgaggc gctctccacg ctggcggaac tggaagatat cggctggaaa gttcgcctgt
    19381 gtctgatgga tacccagcgc gcgctcaact tcctggtacg caaggcgcgt ttaccgggtg
    19441 ggcaactgga gcaggcgcgt gaaatcctgc gagatatcga atccctgctg ccgcataacg
    19501 aatccctgtt ccagaaggtg aacttcctga tgcaggcggc gatgggcttt atcaacatcg
    19561 agcagaaccg catcatcaaa atcttctcgg tggtatccgt ggtgttcctg ccaccgacgc
    19621 tggtggcttc cagctacggg atgaactttg aatttatgcc agagttgaag tggagtttcg
    19681 gctacccagg cgcgattatc tttatgatcc tcgcgggcct ggcaccgtat ctgtacttta
    19741 agcggaagaa ctggttgtaa tatgagtgcc ggataaagct atttttttat ccggcttatt
    19801 tctaaaaatt gtctttttta aagtcatgcg gattgaaaac tcgctctatt ttattttctt
    19861 gtgttaat
//
LOCUS       DAZKHF020000068        18913 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2201S31241
            SAMN43267800-rid25106413.denovo.068, whole genome shotgun sequence.
ACCESSION   DAZKHF020000068 DAZKHF020000000
VERSION     DAZKHF020000068.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267800
            Sequence Read Archive: SRR30307263
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 18913)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18913)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 18913)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350581.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350581.2
            Genome Coverage       :: 151x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:21:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,652
            CDSs (total)                      :: 5,557
            Genes (coding)                    :: 5,320
            CDSs (with protein)               :: 5,320
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 237
            CDSs (without protein)            :: 237
            Pseudo Genes (ambiguous residues) :: 0 of 237
            Pseudo Genes (frameshifted)       :: 102 of 237
            Pseudo Genes (incomplete)         :: 130 of 237
            Pseudo Genes (internal stop)      :: 52 of 237
            Pseudo Genes (multiple problems)  :: 41 of 237
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18913
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267800-rid25106413.denovo.068"
                     /serotype="O 26:H11"
                     /isolate="2201S31241"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-01"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            6..2396
                     /gene="gcd"
                     /locus_tag="ACHOSC_RS09950"
                     /old_locus_tag="pgaptmp_001990"
     CDS             6..2396
                     /gene="gcd"
                     /locus_tag="ACHOSC_RS09950"
                     /old_locus_tag="pgaptmp_001990"
                     /EC_number="1.1.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinoprotein glucose dehydrogenase"
                     /protein_id="HGU4547932.1"
                     /translation="MAINNTGSRRLLVTLTALFAALCGLYLLIGGGWLVAIGGSWSYP
                     IAGLVMLGVAWMLWRSKRAALWLYAALLLGTMIWGVWEVGFDFWALTPRSDILVFFGI
                     WLILPFVWRRLVIPASGAVAALVVALLISGSILTWAGFNDPQEINGTLSADATPAEAI
                     SPVADQDWPAYGRNQEGQRFSPLKQINADNVHNLKEAWVFRTGDVKQPNDPGEITNEV
                     TPIKVGDTLYLCTAHQRLFALDAASGKEKWHYDPELKTNESFQHVTCRGVSYHEAKAE
                     TASPEVMADCPRRIILPVNDGRLIAINAENGKLCETFANKGVLNLQSNMPDTKPGLYE
                     PTSPPIITDKTIVMAGSVTDNFSTRETSGVIRGFDVNTGELLWAFDPGAKDPNAIPSD
                     EHTFTFNSPNSWAPAAYDAKLDLVYLPMGVTTPDIWGGNRTPEQERYASSILALNATT
                     GKLAWSYQTVHHDLWDMDLPAQPTLADITVNGQKVPVIYAPAKTGNIFVLDRRNGELV
                     VPAPEKPVPQGAAKGDYVTPTQPFSELSFRPKKDLSGADMWGATMFDQLVCRVMFHQM
                     RYEGIFTPPSEQGTLVFPGNLGMFEWGGISVDPNREVAIANPMALPFVSKLIPRGPGN
                     PMEQPKDAKGTGTESGIQPQYGVPYGVTLNPFLSPFGLPCKQPAWGYISALDLKTNEV
                     VWKKRIGTPQDSMPFPMPVPVPFNMGMPMLGGPISTAGNVLFIAATADNYLRAYNMSN
                     GEKLWQGRLPAGGQATPMTYEVNGKQYVVISAGGHGSFGTKMGDYIVAYALPDDVK"
     gene            complement(2443..3993)
                     /gene="cueO"
                     /locus_tag="ACHOSC_RS09955"
                     /old_locus_tag="pgaptmp_001991"
     CDS             complement(2443..3993)
                     /gene="cueO"
                     /locus_tag="ACHOSC_RS09955"
                     /old_locus_tag="pgaptmp_001991"
                     /EC_number="1.16.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005507 - copper ion binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multicopper oxidase CueO"
                     /protein_id="HGU4547933.1"
                     /translation="MQRRDFLKYSVALGVASALPLWSRAVFAAERPTLPIPDLLTTDA
                     RNRIQLTIGAGQSTFGEKTATTWGYNGNLLGPAVKLQRGKAVTVDIYNQLTEETTLHW
                     HGLEVPGEVDGGPQGIILPGGKRSVTLNVDQPAATCWFHPHQHGKTGRQVAMGLAGLV
                     VIEDDEILKLMLPKQWGIDDVPVIVQDKKFNADGQIDYQLDVMTAAVGWFGDTLLTNG
                     AIYPQHAAPRGWLRLRLLNGCNARSLNFATSDNRPLYVIASDGGLLPEPVKVSELPVL
                     MGERFEVLVEVNDNKPFDLVTLPVSQMGMAIAPFDKPHPVMRIQPIAISASGALPDTL
                     SSLPALPSLEGLTVRKLQLSMDPMLDMMGMQMLMEKYGDQAMAGMDHSQMMGHMGHGN
                     MNHMNHGGKFDFHHANKINGQAFDMNKPMFAAAKGQYERWVISGVGDMMLHPFHIHGT
                     QFRILSENGKPPAAHRAGWKDTVKVEGNVSEVLVKFNHNAPKEHAYMAHCHLLEHEDT
                     GMMLGFTV"
     gene            4159..4506
                     /gene="yacC"
                     /locus_tag="ACHOSC_RS09960"
                     /old_locus_tag="pgaptmp_001992"
     CDS             4159..4506
                     /gene="yacC"
                     /locus_tag="ACHOSC_RS09960"
                     /old_locus_tag="pgaptmp_001992"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414664.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YacC family pilotin-like protein"
                     /protein_id="HGU4547934.1"
                     /translation="MKTFFRTVLFGSLMAVCANSYALSESEAEDMADLTAVFVFLKND
                     CGYQNLPNGQIRRALVFFAQQNQWDLSNYDTFDMKALGEDSYRDLSGIGIPVAKKCKA
                     LARDSLSLLAYVK"
     gene            4612..5478
                     /gene="speE"
                     /locus_tag="ACHOSC_RS09965"
                     /old_locus_tag="pgaptmp_001993"
     CDS             4612..5478
                     /gene="speE"
                     /locus_tag="ACHOSC_RS09965"
                     /old_locus_tag="pgaptmp_001993"
                     /EC_number="2.5.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyamine aminopropyltransferase"
                     /protein_id="HGU4547935.1"
                     /translation="MAEKKQWHETLHDQFGQYFAVDNVLYHEKTDHQDLIIFENAAFG
                     RVMALDGVVQTTERDEFIYHEMMTHVPLLAHGHAKHVLIIGGGDGAMLREVTRHKNVE
                     SITMVEIDAGVVSFCRQYLPNHNAGSYDDPRFKLVIDDGVNFVNQTSQTFDVIISDCT
                     DPIGPGESLFTSAFYEGCKRCLNPGGIFVAQNGVCFLQQEEAIDSHRKLSHYFSDVGF
                     YQAAIPTYYGGIMTFAWATDNDALRHLSTEIIQARFLASGLKCRYYNPAIHTAAFALP
                     QYLQDALASQPS"
     gene            5494..6288
                     /gene="speD"
                     /locus_tag="ACHOSC_RS09970"
                     /old_locus_tag="pgaptmp_001994"
     CDS             5494..6288
                     /gene="speD"
                     /locus_tag="ACHOSC_RS09970"
                     /old_locus_tag="pgaptmp_001994"
                     /EC_number="4.1.1.50"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004014 - adenosylmethionine decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0008295 - spermidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylmethionine decarboxylase"
                     /protein_id="HGU4547936.1"
                     /translation="MKKLKLHGFNNLTKSLSFCIYDICYAKTAEERDGYIAYIDELYN
                     ANRLTEILSETCSIIGANILNIARQDYEPQGASVTILVSEEPVDPKLIDKTEHPGPLP
                     ETVVAHLDKSHICVHTYPESHPEGGLCTFRADIEVSTCGVISPLKALNYLIHQLESDI
                     VTIDYRVRGFTRDINGMKHFIDHEINSIQNFMSDDMKALYDMVDVNVYQENIFHTKML
                     LKEFDLKHYMFHTKPEDLTDSERQEITAALWKEMREIYYGRNMPAV"
     gene            complement(6326..6688)
                     /gene="yacL"
                     /locus_tag="ACHOSC_RS09975"
                     /old_locus_tag="pgaptmp_001995"
     CDS             complement(6326..6688)
                     /gene="yacL"
                     /locus_tag="ACHOSC_RS09975"
                     /old_locus_tag="pgaptmp_001995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414661.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YacL"
                     /protein_id="HGU4547937.1"
                     /translation="MDYEFLRDITGVVKVRMSMGHEVVGHWFNEEVKENLALLDEVEQ
                     AAHALKGSERSWQRAGHEYTLWMDGEEVMVRANQLEFAGDEMEEGMNYYDEESLSLCG
                     VEDFLQVVAAYRNFVQQK"
     gene            complement(6863..9460)
                     /gene="acnB"
                     /locus_tag="ACHOSC_RS09980"
                     /old_locus_tag="pgaptmp_001996"
     CDS             complement(6863..9460)
                     /gene="acnB"
                     /locus_tag="ACHOSC_RS09980"
                     /old_locus_tag="pgaptmp_001996"
                     /EC_number="4.2.1.3"
                     /EC_number="4.2.1.99"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0003994 - aconitate hydratase activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional aconitate hydratase
                     2/2-methylisocitrate dehydratase"
                     /protein_id="HGU4547938.1"
                     /translation="MLEEYRKHVAERAAEGIAPKPLDANQMAALVELLKNPPAGEEEF
                     LLDLLTNRVPPGVDEAAYVKAGFLAAIAKGEAKSPLLTPEKAIELLGTMQGGYNIHSL
                     IDALDDAKLAPIAAKALSHTLLMFDNFYDVEEKAKAGNEYAKQVMQSWADAEWFLNRP
                     ALAEKLTVTVFKVTGETNTDDLSPAPDAWSRPDIPLHALAMLKNAREGIEPDQPGVVG
                     PIKQIEALQQKGFPLAYVGDVVGTGSSRKSATNSVLWFMGDDIPHVPNKRGGGLCLGG
                     KIAPIFFNTMEDAGALPIEVDVSNLNMGDVIDVYPYKGEVRNHETGELLATFELKTDV
                     LIDEVRAGGRIPLIIGRGLTTKAREALGLPHSDVFRQAKDVAESDRGFSLAQKMVGRA
                     CGVKGIRPGAYCEPKMTSVGSQDTTGPMTRDELKDLACLGFSADLVMQSFCHTAAYPK
                     PVDVNTHHTLPDFIMNRGGVSLRPGDGVIHSWLNRMLLPDTVGTGGDSHTRFPIGISF
                     PAGSGLVAFAAATGVMPLDMPESVLVRFKGKMQPGITLRDLVHAIPLYAIKQGLLTVE
                     KKGKKNIFSGRILEIEGLPDLKVEQAFELTDASAERSAAGCTIKLNKEPIIEYLNSNI
                     VLLKWMIAEGYGDRRTLERRIQGMEKWLANPELLEADADAEYAAVIDIDLADIKEPIL
                     CAPNDPDDARPLSAVQGEKIDEVFIGSCMTNIGHFRAAGKLLDAHKGQLPTRLWVAPP
                     TRMDAAQLTEEGYYSVFGKSGARIEIPGCSLCMGNQARVADGATVVSTSTRNFPNRLG
                     TGANVFLASAELAAVAALIGKLPTPEEYQTYVAQVDKTAVDTYRYLNFNQLSQYTEKA
                     DGVIFQTAV"
     gene            9815..11476
                     /locus_tag="ACHOSC_RS09985"
                     /old_locus_tag="pgaptmp_001997"
     CDS             9815..11476
                     /locus_tag="ACHOSC_RS09985"
                     /old_locus_tag="pgaptmp_001997"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3300 domain-containing protein"
                     /protein_id="HGU4547939.1"
                     /translation="MKMTLPFKPHVLALICSAGLCAASTGLYIKSRTVEAPVEPQSTQ
                     QTAPDITAVTLPATVSAPPVTPAVVKSAFSTAQIDQWVAPVALYPDSLLSQVLMASTY
                     PANVAQAVQWSHDNPLKQGDAAIQAVSDQPWDASVKSLVAFPQLMALMGENPQWVQNL
                     GDAFLAQPQDVMDSVQRLRQLAQQTGSLKSSTEQKVITTTKKAVPVKQTVTAPVIPSN
                     TVLTASPVITEPATTVISIEPANPDVVYIPNYNPTVVYGNWANTAYPPVYLPPPAGEP
                     FIDSFVRGFGYSMGVATTYALFSSIDWDDDDHDHHHHDDDDYHHHDGGHRDGNGWQHN
                     GDNINIDVNNFNRITGEHLTDKNMAWRHNPNYRNGVPYHDQDMAKRFHQTDVNGGMSA
                     TQLPAPTRDSQRQAAASQFQQRTHAAPVITRDTQRQAAAQRFNEAENYGSYDDFRDFS
                     RRQPLTQQQKDAARQRYQSASPEQRQVFKEKVQQRPLNQQQRDNARQRVQSASPEQRQ
                     VFREKVQESRPQRLNDSNHTVRLNNEQRSAVRERLSERGARRQER"
     gene            complement(11547..12971)
                     /gene="lpdA"
                     /locus_tag="ACHOSC_RS09990"
                     /old_locus_tag="pgaptmp_001998"
     CDS             complement(11547..12971)
                     /gene="lpdA"
                     /locus_tag="ACHOSC_RS09990"
                     /old_locus_tag="pgaptmp_001998"
                     /EC_number="1.8.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001738005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004148 - dihydrolipoyl dehydrogenase
                     activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyl dehydrogenase"
                     /protein_id="HGU4547940.1"
                     /translation="MSTEIKTQVVVLGAGPAGYSAAFRCADLGLETVIVERYNTLGGV
                     CLNVGCIPSKALLHVAKVIEEAKALAEHGIVFGEPKTDIDKIRTWKEKVINQLTGGLA
                     GMAKGRKVKVVNGLGKFTGANTLEVEGENGKTVINFDNAIIAAGSRPIQLPFIPHEDP
                     RIWDSTDALELKEVPERLLVMGGGIIGLEMGTVYHALGSQIDVVEMFDQVIPAADKDI
                     VKVFTKRISKKFNLMLETKVTAVEAKEDGIYVTMEGKKAPAEPQRYDAVLVAIGRVPN
                     GKNLDAGKAGVEVDDRGFIRVDKQLRTNVPHIFAIGDIVGQPMLAHKGVHEGHVAAEV
                     IAGKKHYFDPKVIPSIAYTEPEVAWVGLTEKEAKEKGISYETATFPWAASGRAIASDC
                     ADGMTKLIFDKESHRVIGGAIVGTNGGELLGEIGLAIEMGCDAEDIALTIHAHPTLHE
                     SVGLAAEVFEGSITDLPNPKAKKK"
     gene            complement(13296..15188)
                     /gene="aceF"
                     /locus_tag="ACHOSC_RS09995"
                     /old_locus_tag="pgaptmp_001999"
     CDS             complement(13296..15188)
                     /gene="aceF"
                     /locus_tag="ACHOSC_RS09995"
                     /old_locus_tag="pgaptmp_001999"
                     /EC_number="2.3.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045254 - pyruvate dehydrogenase complex
                     [Evidence IEA];
                     GO_function: GO:0004742 - dihydrolipoyllysine-residue
                     acetyltransferase activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate dehydrogenase complex
                     dihydrolipoyllysine-residue acetyltransferase"
                     /protein_id="HGU4547941.1"
                     /translation="MAIEIKVPDIGADEVEITEILVKVGDKVEAEQSLITVEGDKASM
                     EVPSPQAGIVKEIKVSVGDKTQTGALIMIFDSADGAADAAPAQAEEKKEAAPAAAPAA
                     AAAKDVNVPDIGSDEVEVTEILVKVGDKVEAEQSLITVEGDKASMEVPAPFAGTVKEI
                     KVNVGDKVSTGSLIMVFEVAGEAGAAAPAAKQEAAPAAAPAPAAGVKEVNVPDIGGDE
                     VEVTEVMVKVGDKVAAEQSLITVEGDKASMEVPAPFAGVVKELKVNVGDKVKTGSLIM
                     IFEVEGAAPAAAPAKQEAAAPAPAAKAEAPAAAPAAKAEGKSEFAENDAYVHATPLIR
                     RLAREFGVNLAKVKGTGRKGRILREDVQAYVKEAIKRAEAAPAATGGGIPGMLPWPKV
                     DFSKFGEIEEVELGRIQKISGANLSRNWVMIPHVTHFDKTDITELEAFRKQQNEEAAK
                     RKLDVKITPVVFIMKAVAAALEQMPRFNSSLSEDGQRLTLKKYINIGVAVDTPNGLVV
                     PVFKDVNKKGIIELSRELMTISKKARDGKLTAGEMQGGCFTISSIGGLGTTHFAPIVN
                     APEVAILGVSKSAMEPVWNGKEFVPRLMLPISLSFDHRVIDGADGARFITIINNTLSD
                     IRRLVM"
     gene            complement(15203..17866)
                     /gene="aceE"
                     /locus_tag="ACHOSC_RS10000"
                     /old_locus_tag="pgaptmp_002000"
     CDS             complement(15203..17866)
                     /gene="aceE"
                     /locus_tag="ACHOSC_RS10000"
                     /old_locus_tag="pgaptmp_002000"
                     /EC_number="1.2.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008814576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045254 - pyruvate dehydrogenase complex
                     [Evidence IEA];
                     GO_function: GO:0004738 - pyruvate dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0006086 - acetyl-CoA biosynthetic process
                     from pyruvate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate dehydrogenase (acetyl-transferring),
                     homodimeric type"
                     /protein_id="HGU4547942.1"
                     /translation="MSERFPNDVDPIETRDWLQAIESVIREEGVERAQYLIDQLLAEA
                     RKGGVNVAAGTGISNYINTIPVEEQPEYPGNLELERRIRSAIRWNAIMTVLRASKKDL
                     ELGGHMASFQSSATIYDVCFNHFFRARNEQDGGDLVYFQGHISPGVYARAFLEGRLTQ
                     EQLDNFRQEVHGNGLSSYPHPKLMPEFWQFPTVSMGLGPIGAIYQAKFLKYLEHRGLK
                     DTSKQTVYAFLGDGEMDEPESKGAITIATREKLDNLVFVINCNLQRLDGPVTGNGKII
                     NELEGIFEGAGWNVIKVMWGSRWDELLRKDTSGKLIQLMNETVDGDYQTFKSKDGAYV
                     REHFFGKYPETAALVADWTDEQIWALNRGGHDPKKIYAAFKKAQETKGKATVILAHTI
                     KGYGMGDAAEGKNIAHQVKKMNMDGVRHIRDRFNVPVSDADIEKLPYITFPEGSEEHT
                     YLHAQRQKLHGYLPSRQPNFTEKLELPSLQDFGALLEEQSKEISTTIAFVRALNVMLK
                     NKSIKDRLVPIIADEARTFGMEGLFRQIGIYSPNGQQYTPQDREQVAYYKEDEKGQIL
                     QEGINELGAGCSWLAAATSYSTNNLPMIPFYIYYSMFGFQRIGDLCWAAGDQQARGFL
                     IGGTSGRTTLNGEGLQHEDGHSHIQSLTIPNCISYDPAYAYEVAVIMHDGLERMYGEK
                     QENVYYYITTLNENYHMPAMPEGAEEGIRKGIYKLETIEGSKGKVQLLGSGSILRHVR
                     EAAEILAKDYGVGSDVYSVTSFTELARDGQDCERWNMLHPLETPRVPYIAQVMNDAPA
                     VASTDYMKLFAEQVRTYVPADDYRVLGTDGFGRSDSRENLRHHFEVDASYVVVAALGE
                     LAKRGEIDKKVVADAIAKFNIDADKVNPRLA"
     gene            complement(18027..18791)
                     /gene="pdhR"
                     /locus_tag="ACHOSC_RS10005"
                     /old_locus_tag="pgaptmp_002001"
     CDS             complement(18027..18791)
                     /gene="pdhR"
                     /locus_tag="ACHOSC_RS10005"
                     /old_locus_tag="pgaptmp_002001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate dehydrogenase complex transcriptional
                     repressor PdhR"
                     /protein_id="HGU4547943.1"
                     /translation="MAYSKIRQPKLSDVIEQQLEFLILEGTLRPGEKLPPERELAKQF
                     DVSRPSLREAIQRLEAKGLLLRRQGGGTFVQSSLWQSFSDPLVELLSDHPESQYDLLE
                     TRHALEGIAAYYAALRSTDEDKERIRELHHAIELAQQSGDLDAESNAVLQYQIAVTEA
                     AHNVVLLHLLRCMEPMLAQNVRQNFELLYSRREMLPLVSSHRTRIFEAIMAGKPEEAR
                     EASHRHLAFIEEILLDRSREESRRERSLRRLEQRKN"
BASE COUNT         4322 a         5266 c         4756 g         4569 t
ORIGIN      
        1 tctttatggc aattaacaat acaggctcgc gacgattact cgtcacgcta acagcccttt
       61 ttgcagcgct ttgcgggctg tatctactca ttggcggagg ctggctggtc gcgattggcg
      121 gctcctggtc ctaccctatc gctggccttg tgatgctcgg cgtcgcctgg atgctgtggc
      181 gcagtaaacg tgccgcgctt tggctatacg ccgccctgct gctcggcacc atgatttggg
      241 gcgtctggga agttggtttc gacttctggg cgctgactcc gcgcagcgat attctggtct
      301 tcttcggcat ctggctgatc ctgccgtttg tctggcgtcg cctggtcatt cctgccagcg
      361 gcgcagttgc cgcactggtg gtggcactgc tgattagcgg tagtatcctg acctgggccg
      421 gatttaacga tccgcaggaa atcaacggca ccttaagcgc cgatgccaca cctgcggaag
      481 ctatctcacc cgttgccgat caggactggc ctgcctatgg tcgcaatcag gaaggtcaac
      541 gcttttcacc tctgaaacaa attaacgccg ataacgtcca taatctgaaa gaagcctggg
      601 tgttccgtac tggcgatgtg aagcagccga acgatccggg cgaaatcacc aatgaagtga
      661 cgccgattaa agtgggcgac accctttacc tgtgtaccgc tcaccagcgc ctgtttgcgc
      721 tcgatgccgc cagcggcaaa gagaaatggc attacgatcc tgagctgaaa actaacgagt
      781 ctttccagca cgtaacctgc cgtggcgtct cttatcatga agccaaagca gaaacggctt
      841 cgccggaagt gatggcggat tgcccgcgtc gtatcattct tccggtcaat gatggtcgac
      901 tgattgcgat taacgctgaa aacggcaaac tgtgcgaaac cttcgccaat aaaggcgtgc
      961 tcaatctgca aagcaatatg ccagacacca aaccgggtct gtatgaaccg acttcgccac
     1021 cgattatcac cgataaaacc atcgtgatgg caggttcagt taccgataac ttctcaaccc
     1081 gcgaaacgtc tggcgtgatc cgtggttttg atgtcaacac cggggagctg ctgtgggctt
     1141 ttgatcccgg cgcgaaagat ccgaacgcaa tcccgtctga cgaacacacc tttaccttta
     1201 actcgccaaa ctcgtgggcg ccagcggcct atgacgcgaa gctggatctg gtctatctgc
     1261 cgatgggcgt gaccacgccg gatatctggg gcggtaaccg cacaccggaa caggaacgtt
     1321 atgccagctc gattctggcg ctgaatgcca ctaccgggaa actggcgtgg agctaccaga
     1381 ccgttcacca cgacctgtgg gacatggatc ttccggcaca gccgacgctg gcggacatca
     1441 ccgttaatgg tcagaaagtg ccagttattt acgctccggc gaaaaccggc aacatttttg
     1501 tgctcgatcg tcgcaatggc gaactggtgg ttccggcccc ggaaaaaccg gtgccgcaag
     1561 gtgctgcgaa aggcgattac gttactccga cccagccgtt ctctgaactg agcttccgtc
     1621 cgaagaaaga tttgagcggt gcggatatgt ggggagccac catgtttgac caactggtgt
     1681 gccgcgtgat gttccaccag atgcgctatg aaggcatttt cacgccgcca tctgaacagg
     1741 gcacgctggt cttcccgggt aacctgggaa tgtttgaatg gggcgggatt tccgttgatc
     1801 caaatcgtga agtggcaatt gccaacccaa tggcactgcc gtttgtttcg aaactgatcc
     1861 cacgcggtcc gggtaacccg atggagcagc cgaaagatgc caaaggcacg ggtacggaat
     1921 ccggcattca gccacagtac ggtgtaccgt atggtgtcac gctcaacccg ttcctctcac
     1981 cgtttggtct gccgtgtaaa cagccagcat ggggttatat ctcggcgctg gatctgaaaa
     2041 ccaatgaagt ggtgtggaag aaacgtattg gtacgccgca agacagtatg ccgttcccga
     2101 tgcctgtacc ggtgccgttc aatatgggta tgccgatgct gggcgggcca atctccacgg
     2161 cgggtaacgt gctgtttatc gctgctacgg cagataacta cctgcgcgct tacaacatga
     2221 gcaacggtga aaaactgtgg cagggccgtt taccagcggg tggtcaggcg acgccaatga
     2281 cctatgaagt gaatggcaag cagtatgtgg tgatctccgc aggcggtcac ggttcatttg
     2341 gtacgaagat gggcgactat attgtggctt atgcgctgcc ggatgatgtg aaataaccat
     2401 aatgcccgga gagatccggg catatttccg aatacggtct ttttataccg taaaccctaa
     2461 catcatcccc gtatcttcat gctccagcag atggcagtgc gccatataag catgttcttt
     2521 cggtgcgttg tgattaaact tcaccagcac ttcactgaca ttgccttcta ccttaacggt
     2581 atctttccag cccgcgcgat gagccgctgg cggtttgcca ttttctgaca agatacggaa
     2641 ctgggtgccg tgaatatgga acggatgcag catcatgtcg cccacgccag agataaccca
     2701 acgttcgtac tgccctttcg ccgccgcaaa catcggctta ttcatatcaa acgcctgacc
     2761 gttgatttta ttggcatggt ggaaatcgaa cttcccgcca tggttcatat gattcatatt
     2821 gccgtgcccc atatggccca tcatctggct atgatccatc ccggccatcg cctgatcgcc
     2881 atatttctcc atcagcatct gcatccccat catatcgagc atcgggtcca tagaaagttg
     2941 cagcttgcgt accgtcagcc cttccagcga aggtaacgca ggcaggctac ttaatgtgtc
     3001 tggcaaagca ccggaggcac taatagcaat cggctgaatc cgcattaccg gatgcggctt
     3061 gtcaaacggc gcaatcgcca tccccatctg gctgaccggt agcgtcacca ggtcaaaggg
     3121 tttgttgtcg ttaacctcca ccagcacttc aaaacgctcg cccatcagca ccggcagctc
     3181 gctcaccttc accggttcag gtagcagacc accgtcgctg gcaatcacat acagcgggcg
     3241 attgtcgctg gtggcgaaat tgagcgagcg ggcattacag ccattaagca aacgcaggcg
     3301 cagccaacca cgcggggcag cgtgttgtgg gtagattgca ccgttggtca gcagcgtatc
     3361 gccaaaccag cccacggcgg cggtcatcac atccagttga taatcaatct gcccatcagc
     3421 gttaaatttt ttatcctgaa caatcaccgg aacatcatcg atgccccact gttttggcag
     3481 cattaatttc aggatctcgt catcttcaat taccaccagc ccagccaacc ccatcgccac
     3541 ctgtcgcccg gtcttgccat gttgatgtgg atggaaccag caagtagcgg caggttgatc
     3601 aacgttcaac gtcaccgagc gcttgccacc tggcagaata attccctgcg ggccgccgtc
     3661 gacttcaccc ggtacttcca gcccgtgcca gtgcaacgtc gtctcttccg tcagttggtt
     3721 atagatatca accgttaccg ctttgccgcg ctgtaatttc accgccggcc ccagcagatt
     3781 gccgttatag ccccaggtag ttgcagtttt ctcgccaaag gtagactgac ctgcgccaat
     3841 agttaactga atgcgattac gggcatcggt cgtgagcaaa tcagggatcg gtaacgttgg
     3901 gcgttccgcc gcaaagactg cgcggctcca cagcggcaag gctgaagcca cacccagcgc
     3961 gacggaatat tttaagaaat cacgacgttg catagttatt tccttattct taagcaggcg
     4021 aaacaaaatc aaacgttgag catagtcctt ccccttacgg gaaggtcaag ccgcagacac
     4081 aatttaatcg acaataatgg tcgtcgcctc gctcgcagtg tgctaacgtt tatcttcttt
     4141 aagcccctgg tagaagcaat gaagacgttt ttcagaacag tgttattcgg cagcctgatg
     4201 gccgtttgcg caaacagtta cgcgctcagc gagtctgaag ccgaagatat ggccgattta
     4261 acggcagttt ttgtctttct gaagaacgat tgtggttacc agaacttacc taacgggcaa
     4321 attcgtcgcg cactggtctt tttcgcccag caaaaccagt gggatctcag taattacgac
     4381 accttcgaca tgaaagccct cggtgaagac agctaccgcg atctcagcgg cattggcatt
     4441 cccgtcgcta aaaagtgcaa agccctggcc cgcgattcct taagcctgct tgcctacgtc
     4501 aaataatccc tgataccttt ttgtagaaat attgaccgtg catccgcggt caatgttagc
     4561 tattatgttg cgcccttttt ttacgggtgt taacaaagga ggtatcaacc catggccgaa
     4621 aaaaaacagt ggcatgaaac gctacacgac cagtttgggc agtactttgc ggtagataac
     4681 gttctgtatc atgaaaagac cgatcaccag gatctgatca tttttgagaa cgctgcattt
     4741 ggtcgcgtaa tggcgctgga tggcgtagta caaaccaccg agcgcgacga gtttatctat
     4801 catgagatga tgacccatgt tccgctactg gcccacggtc acgcgaaaca tgtgctgatt
     4861 atcggcggcg gcgacggtgc catgctgcgt gaagtaaccc gacataaaaa cgttgagtca
     4921 atcacgatgg tggaaatcga tgcgggtgtc gtgtcgttct gccgtcagta tctgcccaac
     4981 cataacgccg gtagctacga cgatccgcgc tttaagctgg tgatcgacga tggcgtcaat
     5041 ttcgttaatc aaaccagcca gacctttgat gtcattatct ccgactgcac cgatcctatc
     5101 ggtcccggcg aaagtctgtt cacctcagca ttttatgaag gctgcaaacg ttgcctgaat
     5161 cctggcggta tcttcgtcgc acaaaacggc gtctgctttt tacagcagga agaagccatc
     5221 gacagccatc gcaaactcag ccattacttc agcgacgttg gcttttatca ggcggcgatc
     5281 ccgacctatt acggcggtat catgactttt gcatgggcga cagataacga cgccttacgc
     5341 catctctcaa ccgaaattat tcaggcgcgt tttctcgctt ctggcctgaa atgccgttat
     5401 tacaatccgg caatccatac ggcagctttt gccttacctc agtatctgca agacgcactg
     5461 gcttcacagc cgtcctaagg agaagataag aaattgaaaa aactgaaact gcatggcttt
     5521 aataatctga ccaaaagtct gagtttttgt atttacgata tctgctacgc caaaactgcc
     5581 gaagagcgcg acggttatat tgcttatatc gatgaactct ataatgccaa ccgtctgacc
     5641 gaaatcctgt cagaaacctg ttccattatc ggggccaata ttcttaacat cgcccgccag
     5701 gattacgaac cacagggtgc cagcgtcact attctggtga gtgaagaacc ggttgacccg
     5761 aaactcatcg acaaaacaga acaccccggc ccactgccag aaacggtcgt tgcccatctc
     5821 gataaaagtc atatttgcgt acatacctac ccggaaagtc atcctgaagg cggtttatgt
     5881 accttccgcg ccgatattga agtctctacc tgcggcgtga tttctccgct gaaggcgctg
     5941 aattacctga tccaccagct tgagtccgat atcgtaacca ttgattatcg cgtgcgcggt
     6001 tttacccgcg acattaacgg tatgaagcac tttatcgacc atgagattaa ttcgattcag
     6061 aactttatgt ctgacgatat gaaggcgctg tatgacatgg tggatgtgaa cgtctatcag
     6121 gaaaatatct tccataccaa gatgttgctt aaagagttcg accttaagca ctacatgttc
     6181 cacaccaaac cggaagattt aaccgacagc gagcgccagg aaattaccgc tgcgctgtgg
     6241 aaagaaatgc gcgagattta ttacgggcgc aatatgccag ctgtttaacg gctctggcgg
     6301 agctcccagg ctccgccaga tttatttact tctgctgcac gaaattgcgg taagccgcca
     6361 cgacctgcag aaaatcctca acgccgcata gcgacaggct ttcttcgtcg tagtagttca
     6421 tcccctcttc catttcatcg ccagcgaatt ccagttgatt ggcgcgaacc atcacctctt
     6481 caccgtccat ccacagggtg tattcatgcc ctgcccgttg ccaggaccgt tcgctacctt
     6541 tcagtgcgtg cgctgcttgt tccacttcat caagcaaggc caggttttct ttcacctctt
     6601 cattaaacca gtgcccgacc acttcatgcc ccatggacat acgcaccttt accactccgg
     6661 taatatcgcg cagaaattcg taatccatag tgtgttcctc tgctcccggc aatgcgccga
     6721 aatcgcattg cgccattgat gtaattatcg cagccttacc ggagaaaaac agcggaacgg
     6781 cgatggttta gaaataaaaa atgcccggga atctcattca ccgggcattg tgtcgtttat
     6841 gcgcagcgcg tgcgcttact tattacaccg cagtctggaa aatcacccca tcggcttttt
     6901 cggtgtactg agaaagctgg ttgaagttca gataacgata agtatcaacg gcagttttat
     6961 ctacttgcgc aacgtaggtc tggtactctt ccggcgtcgg cagtttgcca atcagcgccg
     7021 caacagccgc caattctgca gaagccagga agacattcgc gccagtcccc agacggttcg
     7081 ggaagttacg ggtagaggtg gaaaccaccg tcgcaccgtc tgctacacgc gcctggttac
     7141 ccatacacag ggaacagcca gggatctcga tacgcgcacc gctcttaccg aagacgctat
     7201 agtagccttc ttcggtcaac tgcgcggcat ccatacgggt tggcggtgcc acccacaggc
     7261 gcgtcggcaa ctggcctttg tgcgcatcca gcagtttacc cgcagcacgg aagtgaccga
     7321 tgttggtcat gcaggaaccg ataaacactt cgtcgatctt ctcgccctgt accgcagaca
     7381 gcggacgtgc gtcgtccggg tcgttcggtg cacagaggat tggctcttta atatccgcca
     7441 gatcgatgtc gatcactgcc gcgtattccg catctgcatc ggcttccagc agctcaggat
     7501 tcgccagcca tttttccatg ccctgaatac gacgttccag ggtacgacga tcgccgtaac
     7561 cttccgcgat catccacttc agcaggacga tgttagagtt caggtattcg atgatcggtt
     7621 ctttgttcag cttgatggta caaccagcgg cagaacgctc ggcggacgca tcggtcagtt
     7681 caaatgcctg ctcaactttc agatccggca gaccttcaat ttccagaatg cgaccagaga
     7741 agatgttttt cttgcctttc ttctcaacgg tcagcagacc ttgtttgatc gcatacagcg
     7801 ggatcgcgtg taccagatcg cgcagggtga tgcccggctg cattttgcct ttgaagcgca
     7861 ccagaacgga ttccggcata tccagcggca ttacgcctgt tgcggctgca aatgccacca
     7921 gaccagaacc cgccgggaaa gagataccga tcgggaaacg ggtatgggag tcaccaccgg
     7981 taccgacggt atccggcagc agcatacggt tcagccagga gtggattacg ccgtcacccg
     8041 gacgcagcga cacaccacca cggttcataa tgaagtccgg cagcgtgtgg tgcgtgttca
     8101 cgtcaactgg cttcggatac gccgcggtat ggcagaaaga ctgcatcacc aggtcagccg
     8161 agaagcccag gcacgccagg tctttcagtt catcacgggt catcgggccg gtggtgtcct
     8221 gtgaacctac agaagtcatt ttcggttcgc agtacgcgcc cggacgaata cctttcacac
     8281 cgcaggcacg acctaccatt ttctgcgcca gcgagaagcc gcgatcgctc tcagcgacat
     8341 ctttcgcctg acggaacaca tcactgtgcg gcagaccaag tgcttcacgc gctttggtgg
     8401 tcaggccacg cccgataatc agcgggatac ggccgccagc acgcacttca tcaatcagca
     8461 cgtcggtttt cagttcgaag gtcgccagca gttcgccggt ttcgtggtta cgcacttcac
     8521 ctttgtacgg gtaaacgtca atcacgtcgc ccatgttcag gttagagacg tcgacttcga
     8581 ttggcagtgc acccgcgtct tccatcgtgt taaagaagat gggtgcaatt ttaccgccga
     8641 ggcacaaacc accgccgcgt ttgttcggca catgtggaat atcatcgccc ataaaccaca
     8701 gaacggagtt cgtggcggat ttacgcgatg aacccgtacc cacaacgtca ccgacgtacg
     8761 ccagcgggaa acctttctgt tgcagagctt caatctgttt gatcgggcca acaacacctg
     8821 gctggtctgg ctcaatacct tcacgggcgt ttttcagcat cgccagcgcg tgcagtggga
     8881 tatccgggcg cgaccacgca tccggtgccg gagaaaggtc atcggtgtta gtttcgccag
     8941 tgactttgaa gacagtaacg gtcagttttt cagccagcgc cgggcgattc aggaaccatt
     9001 cggcatccgc ccaggactgc ataacctgct tcgcatattc gttgcctgct ttcgctttct
     9061 cttctacgtc atagaagtta tcgaacatca gcagcgtgtg agaaagtgct ttggcagcaa
     9121 taggtgccag tttggcatca tccagcgcgt cgatcagcga atgaatgttg taaccaccct
     9181 gcatggtgcc cagcagttcg atggcttttt ccggagtcag cagaggggat ttggcttcgc
     9241 ctttcgcgat agcagccagg aagcctgctt tgacataggc ggcttcatcg acgcctgggg
     9301 gaacacggtt ggttaacaga tctaacagga attcttcttc gcccgcgggc gggtttttca
     9361 gcagctctac aagtgcggcc atttggtttg catccagggg tttgggcgca atcccctcag
     9421 cggcacgctc agctacgtgc ttacggtatt cttctagcac gacggttctc ctcgctctca
     9481 ttgtcatagt gcggcaggcg attctcttca cgctcctgtg agacagcagt ttgtagggta
     9541 aatgcccagt accgcaacgg gcagaatagc aggattttga cgatgtgtta atctgtttac
     9601 aaaaaagcaa cataaaaaag tggctgaatc gttaaggatg gtctagagat tgtttccctc
     9661 cgtaaaaact tcgttaattc gcatggtgat aatcaccttt ctcaacgaat cccaagatta
     9721 gtcaaaattt aaactaccgc ctctttatac tcggattcac agcacctgcg ggtggcagtt
     9781 cgccgaccat tgcgatttcc ttgagatccg aattatgaaa atgactttgc cgtttaaacc
     9841 ccatgtgctg gcgctaattt gcagtgccgg gctttgtgcc gcctctaccg ggctatatat
     9901 aaaaagccgc acagtggaag cgcctgtgga accgcaatcg acacaacaga ctgcgcctga
     9961 catcaccgca gttacgcttc ctgcaacggt ttccgcaccg cccgtaacgc ccgccgtcgt
    10021 caaatccgca ttcagcactg cacaaatcga tcaatgggtt gcgcctgtcg cgctgtaccc
    10081 cgattctctg ctttcacaag tgttaatggc atcaacctat ccggcaaacg ttgctcaagc
    10141 agtgcaatgg tcgcacgata atccacttaa acaaggcgat gctgctattc aggcggtatc
    10201 tgaccagccg tgggacgcca gcgttaaatc actggtggcc tttccacaat tgatggcatt
    10261 gatgggcgaa aacccgcaat gggtgcaaaa cctgggcgat gcttttctgg cgcagccgca
    10321 ggacgtgatg gactcggtac agcgattgcg gcaactggcg caacaaaccg gttcgctgaa
    10381 gtcatcaacc gaacaaaaag ttattaccac aacgaagaaa gctgtaccgg taaaacagac
    10441 agtcacggca cccgtcatac catccaatac cgttttaact gccagccccg tcattacaga
    10501 gcctgcaaca accgtcattt ccattgagcc cgccaatcct gatgtggtct atattcccaa
    10561 ctacaaccca accgtggttt acgggaactg ggccaatact gcgtatccgc cggtttatct
    10621 gccaccacca gccggagaac cgtttattga cagctttgtg cgcggattcg gctatagcat
    10681 gggtgttgct accacgtacg cactattcag cagcatcgac tgggatgacg acgatcatga
    10741 ccatcatcat catgacgatg atgattatca tcaccacgat ggcggtcatc gtgacggtaa
    10801 tggctggcaa cacaacggcg acaacatcaa tatcgacgtc aacaatttca accgtatcac
    10861 cggtgagcat cttactgata agaatatggc atggcggcac aatccaaact accgtaatgg
    10921 tgtgccctat catgatcagg atatggcaaa gcggtttcat caaaccgatg tcaacggcgg
    10981 aatgagcgcc acgcagctac ctgctccaac acgcgacagc cagcgtcagg cggcagcaag
    11041 tcagtttcag caacgaacac acgccgcccc cgtcattaca cgagataccc aacgtcaggc
    11101 agcggcacag cggtttaatg aagctgaaaa ctatgggagc tatgacgact tccgcgactt
    11161 cagccgtcgc caaccactga cccagcaaca aaaggacgcc gctcgtcagc gttatcagtc
    11221 ggcctcgcct gagcagcgcc aggtgtttaa ggaaaaagta cagcagcgcc cactgaacca
    11281 acagcaacgt gataacgccc gccagcgtgt tcaatcagca tcacctgaac aacgtcaggt
    11341 ttttcgggaa aaagttcagg agagccgccc acaacgtcta aacgacagta accatactgt
    11401 caggctgaat aacgagcaac ggtcagcagt acgcgaacgt ctctctgagc gcggagcaag
    11461 gcgacaggaa aggtaaattg cagacgtaaa aaaagcggcg tggttagccg cttttttaat
    11521 tgccggatgt tccggcaaac gaacaattac ttcttcttcg ctttcgggtt cggcaggtcg
    11581 gtaatgctac cttcgaacac ttctgccgcc aggcccacag actcgtgcag agtcgggtgc
    11641 gcgtggatgg tcagtgcgat gtcttcagca tcacaaccca tttcgattgc caggccgatt
    11701 tcacccagca gctcgccgcc gttggtaccg acaatcgcac caccgatcac acggtgagat
    11761 tctttgtcga aaatcagctt ggtcataccg tctgcgcagt cggaagcgat agcacgacca
    11821 gaagcagccc acgggaaggt ggcggtttca tagctgatgc ctttctcttt cgcttctttc
    11881 tcagtcagac ctacccatgc aacttctggt tcggtatagg cgatggacgg gataactttc
    11941 ggatcgaagt agtgtttctt accggcgata acttcagcgg caacgtgacc ttcgtgaaca
    12001 cctttgtgtg ccagcatcgg ctgaccgacg atatcgccga tagcaaagat gtgcggtacg
    12061 ttggtacgca gctgtttgtc aacgcggatg aaaccacggt cgtccacttc cacgccagct
    12121 ttgcctgcgt cgaggttttt accgttcggc acacgaccaa tcgctaccag cacggcgtcg
    12181 taacgctgcg gttcagcggg tgcttttttg ccttccatcg tcacataaat accgtcttct
    12241 ttcgcttcaa cggcggtaac tttggtttcc agcatcaggt tgaatttctt gctgatacgc
    12301 ttggtgaaga ctttaacgat gtctttgtca gctgccggga taacctggtc gaacatttca
    12361 accacgtcaa tctgtgaacc cagcgcgtgg tatacggtgc ccatttccag accgatgata
    12421 ccgccaccca ttaccagcag gcgttctggt acttctttca gttccagcgc gtcagtggag
    12481 tcccagatac gcggatcttc atgcggaata aacggcagtt ggatcgggcg agaacccgct
    12541 gcaatgatcg cattgtcgaa gttgatcacg gttttgccgt tctcaccttc aacttccagg
    12601 gtgttagccc cggtgaattt acccagaccg ttgaccactt tgactttgcg gcctttcgcc
    12661 ataccagcca gaccaccggt cagctgattg atcactttct ctttccaggt acgaatcttg
    12721 tcaatatcgg ttttcggttc gccgaagacg ataccgtgtt cagccagcgc tttggcttct
    12781 tcgataactt ttgctacgtg cagcagtgct ttagaaggga tacagccgac gttcaggcaa
    12841 acaccgccaa gggtgttgta acgttctacg attacggttt ccagacctaa atcagcgcaa
    12901 cggaaggcag cggagtaacc tgcggggcct gccccaagta ccacgacctg agttttgatt
    12961 tcagtactca tcatgacctc tatatattta tctccggcgg tcatacccgt cgtctttcag
    13021 gccgcagcgg cgttagcttc acgccctcac cccagtcact tacttatgta agctcctggg
    13081 gattctggcg cttgctacct tgctgcaccc agaaatccat agggtatgtg aagtaatacc
    13141 ctaaccacca ccgggtcgtt ctatccgtcg gtattttaca aaattgttaa caatttttaa
    13201 acaacaaacg gcaaccgatt tgtctattcg ctaataacct caatacattc atgagattac
    13261 cagaaaaaag ccggccgttg ggccggctct tttacttaca tcaccagacg gcgaatgtca
    13321 gacagcgtgt tgttaatgat ggtaatgaaa cgggcaccat cagcaccgtc gatcacgcgg
    13381 tggtcgaagg agagagaaat cggcagcatc agacgcggca cgaactcttt accattccac
    13441 accggctcca tcgcggactt ggaaacgccg aggatagcca cttccggcgc gttcacaatc
    13501 ggcgcgaagt gggtagtacc caggccgccg atgctggaga tggtgaagca accgccctgc
    13561 atttcgcccg cagtcagctt accgtcacgc gctttcttag aaatagtcat cagctcgcga
    13621 gacagctcga tgatgccttt cttgttgacg tctttgaata ccggaacaac cagaccgttc
    13681 ggggtatcca ccgccacacc gatgttgatg tatttcttca gggtcagacg ctgaccgtct
    13741 tccgacaacg aactgttgaa gcgaggcatc tgctcaagag ctgcagcaac ggctttcatg
    13801 atgaagacaa ccggggtgat cttcacatcc agcttacgtt tcgccgcttc ttcgttctgc
    13861 tgtttacgga acgcttccag ctcggtgata tcggttttgt cgaagtgagt aacatgcggg
    13921 atcatcaccc agttacggct caggttcgca ccagaaattt tctggatacg gcccagttcc
    13981 acttcttcga tttcaccaaa cttgctgaag tccaccttcg gccacggcag catgcctggg
    14041 ataccgccgc cagtcgccgc cggagctgct tctgcacgtt tgatagcttc tttcacgtaa
    14101 gcctgaacgt cttcgcgcag gatacgacct ttacggccag tgcccttcac tttcgccagg
    14161 ttaacgccaa actcgcgtgc cagacggcgg atcagcggag tcgcgtgaac gtaagcgtcg
    14221 ttttctgcaa attcagattt gccttccgct ttcgcagccg gtgctgctgc cggagcttca
    14281 gcttttgctg ccggagccgg cgctgccgct tcctgtttcg caggagccgc cgcaggcgct
    14341 gcgccttcaa cttcgaagat cataatcagc gagccagttt tcactttatc gccaacgttg
    14401 actttcagtt ccttcacgac gcctgcaaac ggagccggaa cttccataga agctttgtcg
    14461 ccttctacgg taatcagtga ctgttcagcg gcaactttgt cgcccacttt caccatcact
    14521 tcggtcactt caacttcgtc accgccgata tccggaacgt taacttcttt cacgccagcc
    14581 gctggtgcag gggccgctgc cggagccgct tcctgtttag ccgccggagc tgccgcgcct
    14641 gcttcacccg cgacttcgaa gaccataatc agcgagccgg tagacacttt gtcacccacg
    14701 ttcactttga tctctttcac ggtgccagca aacggagccg gaacttccat agacgcttta
    14761 tcgccttcta cggtgatcag cgactgttca gcttcaactt tatcgccaac tttcaccagg
    14821 atttcggtca cttcaacttc gtcgctgccg atatccggaa cgttaacgtc ttttgccgct
    14881 gcagccgctg gtgctgctgc cggagctgct tctttcttct cttctgcctg agcaggtgca
    14941 gcgtctgctg caccgtcggc ggaatcgaaa atcataatca gtgcgccggt ctgggtttta
    15001 tcgccaacag agactttgat ctctttaacg atacccgcct gcggagacgg aacttccata
    15061 gaggctttgt cgccttctac ggtgatcagc gactgttcgg cttcaacttt gtcgcccact
    15121 ttgaccagga tctcggtgat ttcaacttca tcagccccga tgtccggtac tttgatttcg
    15181 atagccatta ttcttttacc tcttacgcca gacgcgggtt aactttatct gcatcgatgt
    15241 tgaatttggc gattgcgtca gcaaccactt tcttatcgat ttcgccacgt ttagccagtt
    15301 cgcccagcgc cgctacaacc acgtaggaag catcaacttc gaagtggtga cgcaggttct
    15361 cacggctgtc ggaacgaccg aagccatcag tacccagtac gcggtagtcg tcagccggta
    15421 cgtaagtacg gacctgctca gcgaacagtt tcatatagtc ggtagatgcc actgccggag
    15481 cgtcgttcat cacctgagcg atatacggaa cgcgcggagt ttccagcggg tgcagcatgt
    15541 tccagcgttc acaatcctga ccatctcgcg ccagttcggt gaaggaggtc acgctataaa
    15601 cgtcagaacc tacgccgtaa tctttcgcca ggatctctgc cgcttcacgg acgtgacgca
    15661 ggatagaacc ggagcccagc agctgaactt tacctttgct accttcaatg gtttcgagtt
    15721 tgtagatacc tttacggata ccttcctcag caccttccgg cattgccggc atgtggtagt
    15781 tttcgttcag cgtggtgatg tagtagtaaa cgttctcttg tttttcaccg tacatacgct
    15841 ccagaccgtc atgcataatg acagcaactt cgtaagcgta agccgggtcg taagagatac
    15901 agttcggaat agtcagcgac tgaatgtggc tgtgaccatc ttcgtgctgc agaccttcgc
    15961 cgttcagggt ggtacgaccg gaagtaccgc cgatcaggaa gccacgcgct tgctggtcgc
    16021 cagccgccca gcacagatcg ccaatacgct ggaagccgaa catcgagtaa tagatgtaga
    16081 acgggatcat cggcagattg ttggtgctgt aagaggtcgc cgctgccagc caggaacaac
    16141 ctgcgcccag ctcgttgatc ccttcctgca gaatctgacc tttctcgtct tctttatagt
    16201 aagcaacctg ctcgcggtcc tgcggggtgt actgctgacc gttcgggctg taaataccaa
    16261 tctgacggaa cagaccttcc ataccgaaag tacgcgcttc gtcggcgatg atcggtacca
    16321 gacgatcttt gatcgacttg ttcttcagca ttacgttcag ggcacgaacg aaagcgatag
    16381 tggtagagat ctctttgctc tgctcttcca gcagcgcgcc gaagtcttgc aggctcggca
    16441 gctcaagctt ctcggtgaag ttcggctgac ggcttggcag ataaccgtgc agtttctgac
    16501 gctgagcgtg cagataggta tgctcttcag aaccttccgg gaaggtgatg tacggcagtt
    16561 tttcgatatc tgcatcagac accggcacat tgaaacggtc gcggatgtga cgcacgccgt
    16621 ccatgttcat tttcttaacc tggtgcgcga tgtttttacc ttcagccgcg tcgcccatgc
    16681 cgtaaccttt aatggtatga gcaaggatta ctgtcgcttt gcctttggtt tcctgcgctt
    16741 tcttgaatgc agcgtagatt ttcttcggat cgtgaccacc acggttcagt gcccagatct
    16801 gctcgtcagt ccagtctgca accagtgctg cggtttcagg atatttaccg aagaagtgtt
    16861 cacgaacgta cgcaccatct ttcgatttga aggtctggta gtcgccgtca acggtttcgt
    16921 tcatcagctg gatcagttta ccgctggtat ctttacgcag cagttcatcc caacggctac
    16981 cccacatcac tttgatcacg ttccagccag caccttcgaa gatgccttcc agttcgttga
    17041 tgatcttgcc gttaccggtg accgggccgt caagacgctg caggttacag ttgataacga
    17101 agactaggtt atccagtttt tcacgggtag cgatggtgat cgcacctttg gattccggtt
    17161 cgtccatttc accgtcgccg aggaacgcgt aaacggtctg tttagaggta tctttcaggc
    17221 cacggtgttc cagatatttc aggaatttag cctggtaaat agcaccaatc ggccccagac
    17281 ccatagatac ggtcgggaac tgccagaatt ccggcatcag tttcgggtgc ggataggaag
    17341 agaggccatt gccgtgaact tcctgacgga agttatccag ctgctcctga gtcagacgac
    17401 cttctaggaa agcacgagcg tacacgcccg gggagatgtg gccctggaag taaaccaggt
    17461 cgccgccatc ctgctcgttg cgtgcacgga agaagtggtt aaagcacaca tcataaatgg
    17521 ttgcggaaga ctggaaggac gccatatggc cgcccagttc gaggtctttt ttcgacgcac
    17581 gcagcaccgt catgatggcg ttccagcgga tagctgaacg aatacggcgt tccagttcca
    17641 gattacccgg atactccggt tgttcttcaa cggggatggt gttgatgtag ttgctgatac
    17701 ctgtgcctgc ggctacgttt acaccgcctt tgcgggcttc agcaagcagt tggtcgatca
    17761 gatactgagc acgctcaaca ccttcttcac ggatgaccga ttcgatcgcc tggagccagt
    17821 cgcgagtttc gatcggatcc acgtcatttg ggaaacgttc tgacatgggt tattccttat
    17881 ctatctaata acgttgagtt ttctggaacc tgtcccattg aactctcgcc ggaaagctca
    17941 ataagacagg ttctacgttt agttgccgcg cttttatatg cgcttgattt acaacatctt
    18001 ctggataatt tttaccagaa aaatcactaa ttctttcgtt gctccagacg acgcagagaa
    18061 cgctcacggc ggctctcttc acgacttctg tcgagcaaaa tttcttcgat aaaggccaga
    18121 tggcgatgcg atgcttcgcg cgcttcttcc ggcttaccgg ccataatcgc ttcaaatatg
    18181 cgggtgcggt gactactcac cagcggcagc atctcgcgac gcgaatagag caattcgaag
    18241 ttctggcgga cattctgggc caacatcggc tccatacacc ttagcagatg aagcagaacc
    18301 acattgtggg ccgcttcagt gacagcaatc tgatactgga gtacggcgtt tgattccgcg
    18361 tccagatcgc cagactgctg cgccagctct atggcgtggt ggagttcacg gatgcgttcc
    18421 ttgtcttcat cggtactacg cagcgcggcg taataagcgg cgataccttc cagggcgtgt
    18481 cgtgtttcga gcaagtcata ctgtgactca ggatggtcgg agagcagctc caccagcgga
    18541 tcgctgaagc tttgccatag gctgctctgg acaaaagtgc cgccaccctg gcgacgaagc
    18601 aacaagccct tcgcttcgag acgttgaatc gcctcacgca aggagggacg ggagacgtca
    18661 aactgttttg ccagttcgcg ttccggtggg agtttttcgc ccgggcggag agtgccttcg
    18721 aggatcaaaa actccagttg ctgctcaatc acatcggaga gttttggttg gcggattttg
    18781 ctgtaggcca tgagttcctg tcttaagcca cttgccgaag tcaattggtc ttaccaattt
    18841 catgtctgtg acgctaaagt aacaaagtat tcaccttatg tccatacagg ttttgattga
    18901 aatcatgaaa ctg
//
LOCUS       DAZKHF020000069        18801 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2201S31241
            SAMN43267800-rid25106413.denovo.069, whole genome shotgun sequence.
ACCESSION   DAZKHF020000069 DAZKHF020000000
VERSION     DAZKHF020000069.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267800
            Sequence Read Archive: SRR30307263
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 18801)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18801)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 18801)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350581.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350581.2
            Genome Coverage       :: 151x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:21:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,652
            CDSs (total)                      :: 5,557
            Genes (coding)                    :: 5,320
            CDSs (with protein)               :: 5,320
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 237
            CDSs (without protein)            :: 237
            Pseudo Genes (ambiguous residues) :: 0 of 237
            Pseudo Genes (frameshifted)       :: 102 of 237
            Pseudo Genes (incomplete)         :: 130 of 237
            Pseudo Genes (internal stop)      :: 52 of 237
            Pseudo Genes (multiple problems)  :: 41 of 237
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18801
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267800-rid25106413.denovo.069"
                     /serotype="O 26:H11"
                     /isolate="2201S31241"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-01"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            <1..879
                     /locus_tag="ACHOSC_RS10010"
                     /old_locus_tag="pgaptmp_002002"
     CDS             <1..879
                     /locus_tag="ACHOSC_RS10010"
                     /old_locus_tag="pgaptmp_002002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1266 domain-containing protein"
                     /protein_id="HGU4547944.1"
                     /translation="VFAAITSNGLNERGLITAGCSFLYLLIMMGLIVRAGFKAKKEQL
                     HYYQAKGIEPLSIEKLQALQLIAPYRFYHKQWSETLEFWPRKPEPGKDTFQYHVLPFD
                     SIDIISKRRESLEDQWGIEDSESYCALMEYFLSGDHGANTFKANMEEAPEQVIALLNK
                     FAVFPSDYISDCANNRSGKSSAKLIWAAELSWMTSISSTAFQNGTIEEELAWHYIMLA
                     SRKAHELFESEEDYQKNSLMGFLYWHICCYRRKLTDAELEACYRYDKQFWEHYSKKCR
                     WPIRNVPWGASSVKYS"
     gene            complement(996..2339)
                     /gene="gdhA"
                     /locus_tag="ACHOSC_RS10015"
                     /old_locus_tag="pgaptmp_002003"
     CDS             complement(996..2339)
                     /gene="gdhA"
                     /locus_tag="ACHOSC_RS10015"
                     /old_locus_tag="pgaptmp_002003"
                     /EC_number="1.4.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016639 - oxidoreductase activity, acting
                     on the CH-NH2 group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-specific glutamate dehydrogenase"
                     /protein_id="HGU4547945.1"
                     /translation="MDQTYSLESFLNHVQKRDPNQTEFAQAVREVMTTLWPFLEQNPK
                     YRQMSLLERLVEPERVIQFRVVWVDDRNQVQVNRAWRVQFSSAIGPYKGGMRFHPSVN
                     LSILKFLGFEQTFKNALTTLPMGGGKGGSDFDPKGKSEGEVMRFCQALMTELYRHLGA
                     DTDVPAGDIGVGGREVGFMAGMMKKLSNNTACVFTGKGLSFGGSLIRPEATGYGLVYF
                     TEAMLKRHGMGFEGMRVSVSGSGNVAQYAIEKAMEFGARVITASDSSGTVVDESGFTK
                     EKLARLIEIKASRDGRVADYAKEFGLVYLERQQPWSVPVDIALPCATQNELDVDAAHQ
                     LIANGVKAVAEGANMPTTIEATELFQQAGVLFAPGKAANAGGVATSGLEMAQNAARLG
                     WKAEKVDARLHHIMLDIHHACVEHGGEGEQTNYVQGANIAGFVKVADAMLAQGVI"
     gene            2575..2847
                     /locus_tag="ACHOSC_RS10020"
                     /old_locus_tag="pgaptmp_002004"
     CDS             2575..2847
                     /locus_tag="ACHOSC_RS10020"
                     /old_locus_tag="pgaptmp_002004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YnjH family protein"
                     /protein_id="HGU4547946.1"
                     /translation="MSRALFAVVLAFPLIALANPHYRPDVEVNVPPEVFSSGGQSAQP
                     CTQCCVYQDKNYSEGAVIKAEGILLQCQRDDKTLSTNPLVWRRVKP"
     gene            complement(2813..3220)
                     /gene="nudG"
                     /locus_tag="ACHOSC_RS10025"
                     /old_locus_tag="pgaptmp_002005"
     CDS             complement(2813..3220)
                     /gene="nudG"
                     /locus_tag="ACHOSC_RS10025"
                     /old_locus_tag="pgaptmp_002005"
                     /EC_number="3.6.1.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CTP pyrophosphohydrolase"
                     /protein_id="HGU4547947.1"
                     /translation="MKMIEVVAAIIERDGKILLAQRPAQSDQAGLWEFAGGKVEPDES
                     QRQALVRELREELGIEATVGEYVTSHQREVSGRIIHLHAWHVPDFHGTLQAHEHQALV
                     WCSPEEALQYPLAPADIPLLEAFMALRAARPAD"
     gene            3307..3927
                     /locus_tag="ACHOSC_RS10030"
                     /old_locus_tag="pgaptmp_002006"
     CDS             3307..3927
                     /locus_tag="ACHOSC_RS10030"
                     /old_locus_tag="pgaptmp_002006"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707357.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0016780 - phosphotransferase activity, for
                     other substituted phosphate groups [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-alcohol phosphatidyltransferase family
                     protein"
                     /protein_id="HGU4547948.1"
                     /translation="MLDRHLHPRIKPLLHQCVRVLDKPGITPDGLTLVGFAIGVLALP
                     FLALGWYLAALVVILLNRLLDGLDGALARRRGLTDAGGFLDISLDFLFYALVPFGFIL
                     AAPQQNALAGGWLLFAFIGTGSSFLAFAALAAKHQIDNPGYAHKSFYYLGGLTEGTET
                     ILLFVLGCLFPAWFAWFAWIFGALCWMTTFTRVWSGYLTLKSHQRQ"
     gene            complement(3936..5243)
                     /gene="ynjE"
                     /locus_tag="ACHOSC_RS10035"
                     /old_locus_tag="pgaptmp_002007"
     CDS             complement(3936..5243)
                     /gene="ynjE"
                     /locus_tag="ACHOSC_RS10035"
                     /old_locus_tag="pgaptmp_002007"
                     /EC_number="2.8.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416271.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiosulfate sulfurtransferase YnjE"
                     /protein_id="HGU4547949.1"
                     /translation="MKRVSQMTALAMALGLACASSWAAELAKPLTLDQLQQQNGKAID
                     TRPSAFYNGWPQTLNGPSGHEPAALNLSASWLDKMSTEQLNAWIKQHNLKADAPVALY
                     GNDKDVDAVKTRLQKAGFTHISILSDALSEPSRLQKLPHFEQLVYPQWLHDLQQGKEV
                     TAKPAGDWKVIEAAWGAPKLYLISHIPGADYIDTNEVESEPLWNKVSDEQLKAMLAKH
                     GIRHDTTVILYGRDVYAGARVAQIMLYAGVKDVRLLDGGWQTWSDAGLPVERGTPPKV
                     KAEPDFGVKIPAQPQLMLDMEQARGLLHRQDASLVSIRSWPEFIGTTSGYSYIKPKGE
                     IAGARWGHAGSDSTHMEDFHNPDGTMRSADDITAMWKAWNIKPEQQVSFYCGTGWRAS
                     ETFMYARAMGWKNVSVYDGGWYEWSSDPKNPVATGERGPDSSK"
     gene            complement(5310..5963)
                     /locus_tag="ACHOSC_RS10040"
                     /old_locus_tag="pgaptmp_002008"
     CDS             complement(5310..5963)
                     /locus_tag="ACHOSC_RS10040"
                     /old_locus_tag="pgaptmp_002008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HGU4547950.1"
                     /translation="MLCVKNVSLRLPESRLLTNVNFTVDKGDIVTLMGPSGCGKSTLF
                     SWMIGALAGQFSCTGELWLNEQRIDMLPTAQRQIGILFQDALLFDQFSVGQNLLLALP
                     STLKGTARRNAVKDALDRAGLAETYHQDPATLSGGQRARVALLRALLAQPKALLLDEP
                     FSRLDVALRDNFRQWVFSEVRELAIPVVQVTHDLQDVPADSSVLDMEQWSENYNKLR"
     gene            complement(5963..7498)
                     /locus_tag="ACHOSC_RS10045"
                     /old_locus_tag="pgaptmp_002009"
     CDS             complement(5963..7498)
                     /locus_tag="ACHOSC_RS10045"
                     /old_locus_tag="pgaptmp_002009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310488.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HGU4547951.1"
                     /translation="MATPLRYALIFLLWAMVAVIYAPLIPAALTLISPALSLTHWQAL
                     FADPQLPQALLATLVSTTIAAVGALLIALLVIVALWPWPKWQRMCARLPWLLAIPHVA
                     FATSALLLFADGGLLYDYFPYFTPLMDKLGIGLGLTLAVKESAFLLWILAAVLSEKRL
                     LQQVIVLDSLGYSRWQCLNWLLLPSVAPALAMAMLAIVAWSLSVVDVAIILGPGNPPT
                     LAVISWQWLTQGDADQQTKGALASLLLMLLLAAYVLLSYLLWRSWRRTIPRVDGIRKP
                     ATPLLPGTTLASFLPLTGVLCVVLLAILADQSTINSEALINSLTMGLVATFIALLLLL
                     LWLEWGPQRRQLWLWLPTLLPALPLVAGQYTLALWLNLDGSWTAVVWGHLLWVMPWML
                     FILQPAWQRIDSRLILIAQTLSWSRAKIFFYVKCPLMLRPTLIAFAVGFSVSIAQYMP
                     TLWLGAGRFPTLTTEAVALSSGGSNGIFAAQALWQLLLPLIIFALTALVAKWVGYVRQ
                     GLR"
     gene            complement(7471..8637)
                     /locus_tag="ACHOSC_RS10050"
                     /old_locus_tag="pgaptmp_002010"
     CDS             complement(7471..8637)
                     /locus_tag="ACHOSC_RS10050"
                     /old_locus_tag="pgaptmp_002010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416268.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HGU4547952.1"
                     /translation="MRHCGWLLGLLSLFSLATHASDWQEIKNEAKGQTVWFNAWGGDT
                     AINRYLDWVSGEMKTHYAINLKIVRLADAADAVKRIQTEAAAGRKTGGSVDLLWVNGE
                     NFRTLKEANLLQTGWAETLPNWRYVDTQLPVREDFSVPTEGAESPWGGAQLTFIARRD
                     VTPQPPQTPQALLEFAKANPGTVTYPRPPDFTGTAFLEQLLIMLTPDPAALKVAPDKA
                     TFARVTAPLWQYLDALHPYLWRKGKDFPPSPARMDALLKAGTLRLSLTFNPAHAQQKI
                     ASGDLPASSYSFGFNQGMIGNVHFVTIPANANASAAAKVVANFLLSPNAQLRKADPAV
                     WGDPSVLDPQKLPDGQRETLQSRMPQDLPPVLAEPHAGWVNALEQEWLHRYGTH"
     gene            complement(8647..9195)
                     /locus_tag="ACHOSC_RS10055"
                     /old_locus_tag="pgaptmp_002011"
     CDS             complement(8647..9195)
                     /locus_tag="ACHOSC_RS10055"
                     /old_locus_tag="pgaptmp_002011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HGU4547953.1"
                     /translation="MGLPPLSKIPFILRPQAWLHRRHYGEVLSPIRWWGRIPFIFYLV
                     SMFVGWLERKRSPLDPVVRSLVSARIAQMCLCEFCVDITSMKVAERTGSTDKLLAVAD
                     WRQSPLFSDEERLALEYAEAASVTPPTVDDALRTRLAAHFDAQALTELTALIGLQNLS
                     ARFNSAMDIPAQGLCRIPEKRS"
     gene            complement(9195..9902)
                     /locus_tag="ACHOSC_RS10060"
                     /old_locus_tag="pgaptmp_002012"
     CDS             complement(9195..9902)
                     /locus_tag="ACHOSC_RS10060"
                     /old_locus_tag="pgaptmp_002012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TVP38/TMEM64 family protein"
                     /protein_id="HGU4547954.1"
                     /translation="MMKIQSRKIWYYRITLIILLFAMLLAWALLPGVHEFINRSVAAF
                     AAVDQQGIERFIQSYGALAAVVSFLLMILQAIAAPLPAFLITFANASLFGAFWGGLLS
                     WTSSMAGAALCFFIARVMGREVVEKLTGKTVLDSMDGFFTRYGKHTILVCRLLPFVPF
                     DPISYAAGLTSIRFRSFFIATGLGQLPATIVYSWAGSMLTGGTFWFVTGLFILFALTV
                     VIFMAKKIWLERQKRNA"
     gene            complement(9917..10594)
                     /gene="ydjY"
                     /locus_tag="ACHOSC_RS10065"
                     /old_locus_tag="pgaptmp_002013"
     CDS             complement(9917..10594)
                     /gene="ydjY"
                     /locus_tag="ACHOSC_RS10065"
                     /old_locus_tag="pgaptmp_002013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416265.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein YdjY"
                     /protein_id="HGU4547955.1"
                     /translation="MSQHYSVSWKKGLAALCLLAVAGLSGCDQKENAAAKVEYDGLSN
                     SQPLRVDANNHTVTMLVQINGRFLTDDTRHGIVFKDGSNGHKSLFMAYATPKAFYEAL
                     KEAGGTPGENMTMDNKETTHVTGSKLDISVNWQGAAKAYSFDEVIVDSNGKKLDMRFG
                     GNLTAAEEKKTGCLVCLDSCPVGIVSNATYTYGAVEKRGEVKFKGNASVLPADNTLAT
                     VTFKITE"
     gene            complement(10599..11309)
                     /locus_tag="ACHOSC_RS10070"
                     /old_locus_tag="pgaptmp_002014"
     CDS             complement(10599..11309)
                     /locus_tag="ACHOSC_RS10070"
                     /old_locus_tag="pgaptmp_002014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416264.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TVP38/TMEM64 family protein"
                     /protein_id="HGU4547956.1"
                     /translation="MNAERNFLFACLIFALVIYAIHAFGLFDLLTDLPHLQTLIRQSG
                     LFGYSLYILLFIIATLFLLPGSILVIAGGIVFGPLLGTLLSLIAATLASSCSFLLARW
                     LGRDLLLKYVGHSHTFQAIEKGIARNGIDFLILTRLIPLFPYNIQNYAYGLTTIAFWP
                     YTLISALTTLLGIVIYTVMASDLANEGITLRFILQLCLAGLALFILVQLAKLYARHKH
                     VDLSASRRSPLTHPKNEG"
     gene            complement(11476..12282)
                     /gene="xthA"
                     /locus_tag="ACHOSC_RS10075"
                     /old_locus_tag="pgaptmp_002015"
     CDS             complement(11476..12282)
                     /gene="xthA"
                     /locus_tag="ACHOSC_RS10075"
                     /old_locus_tag="pgaptmp_002015"
                     /EC_number="3.1.11.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA];
                     GO_function: GO:0008311 - double-stranded DNA 3'-5' DNA
                     exonuclease activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease III"
                     /protein_id="HGU4547957.1"
                     /translation="MKFVSFNINGLRARPHQLEAIVEKHQPDVIGLQETKVHDDMFPL
                     EEVAKLGYNVFYHGQKGHYGVALLTKETPIAVRRGFPGDDEEAQRRIIMAEIPSLLGN
                     VTVINGYFPQGESRDHPIKFPAKAQFYQNLQNYLETELKRDNPVLIMGDMNISPTDLD
                     IGIGEENRKRWLRTGKCSFLPEEREWMDRLMSWGLVDTFRHANPQTADRFSWFDYRSK
                     GFDDNRGLRIDLLLASQPLAECCVETGIDYEIRSMEKPSDHAPVWATFRR"
     gene            12728..13948
                     /gene="astC"
                     /locus_tag="ACHOSC_RS10080"
                     /old_locus_tag="pgaptmp_002016"
     CDS             12728..13948
                     /gene="astC"
                     /locus_tag="ACHOSC_RS10080"
                     /old_locus_tag="pgaptmp_002016"
                     /EC_number="2.6.1.11"
                     /EC_number="2.6.1.81"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinylornithine/acetylornithine transaminase"
                     /protein_id="HGU4547958.1"
                     /translation="MSQPITRENFDEWMIPVYAPAPFIPVRGEGSRLWDQLGKEYIDF
                     AGGIAVNALGHAHPELREALNEQASKFWHTGNGYTNEPVLRLAKKLIDATFADRVFFC
                     NSGAEANEAALKLARKFAHDRYGSHKSGIVAFKNAFHGRTLFTVSAGGQPAYSQDFAP
                     LPADIRHAAYNDINSASALIDDSTCAVIVEPIQGEGGVVPASNAFLQGLRELCNRHNA
                     LLIFDEVQTGVGRTGELYAYMHYGVTPDLLTTAKALGGGFPVGALLATEECARVMTVG
                     THGTTYGGNPLASAVAGKVLELINTPEMLNGVKQRHDWFVERLNTINHRYGLFSEVRG
                     LGLLIGCVLNADYAGQAKQISQEAAKAGVMVLIAGGNVVRFAPALNVSEEEVTTGLDR
                     FAAACEHFVSRGSS"
     gene            13945..14979
                     /gene="astA"
                     /locus_tag="ACHOSC_RS10085"
                     /old_locus_tag="pgaptmp_002017"
     CDS             13945..14979
                     /gene="astA"
                     /locus_tag="ACHOSC_RS10085"
                     /old_locus_tag="pgaptmp_002017"
                     /EC_number="2.3.1.109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008791 - arginine N-succinyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006527 - arginine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine N-succinyltransferase"
                     /protein_id="HGU4547959.1"
                     /translation="MMVIRPVERSDVSALMQLASKTGGGLTSLPANEATLSARIERAI
                     KTWQGELPKSEQGYVFVLEDSETGTVAGICAIEVAVGLNDPWYNYRVGTLVHASKELN
                     VYNALPTLFLSNDHTGSSELCTLFLDPDWRKEGNGYLLSKSRFMFMAAFRDKFNDKVV
                     AEMRGVIDEHGYSPFWQSLGKRFFSMDFSRADFLCGTGQKAFIAELMPKHPIYTHFLS
                     QEAQDVIGQVHPQTAPARAVLEKEGFRYRNYIDIFDGGPTLECDIDRVRAIRKSRLVE
                     VAEGQPAQGDFPACLVANENYHHFRVVLARTDPATERLILTAAQLDALKCHAGDRVRL
                     VRLCAEEKTA"
     gene            14976..16454
                     /gene="astD"
                     /locus_tag="ACHOSC_RS10090"
                     /old_locus_tag="pgaptmp_002018"
     CDS             14976..16454
                     /gene="astD"
                     /locus_tag="ACHOSC_RS10090"
                     /old_locus_tag="pgaptmp_002018"
                     /EC_number="1.2.1.71"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043824 - succinylglutamate-semialdehyde
                     dehydrogenase activity [Evidence IEA];
                     GO_process: GO:0006527 - arginine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinylglutamate-semialdehyde dehydrogenase"
                     /protein_id="HGU4547960.1"
                     /translation="MTLWINGDWITGQGASRVKRNPVSGEVLWQGNDADAAQVEQACR
                     AARAAFPRWARLSLAERQVVVERFAGLLERNKGELTAIIARETGKPRWEAATEVTAMI
                     NKIAISIKAYHVRTGEQRSEMPDGAASLRHRPHGVLAVFGPYNFPGHLPNGHIVPALL
                     AGNTIIFKPSELTPWSGEAVMRLWQQAGLPPGVLNLVQGGRETGQALSALEDLDGLLF
                     TGSANTGYQLHRQLSGQPEKILALEMGGNNPLIIDEVADIDAAVHLTIQSAFVTAGQR
                     CTCARRLLLKSGAQGDAFLARLVAVSQRLTPGNWDDEPQPFIGGLISEQAAQQVVTAW
                     QQLEAMGGRTLLAPRLLQSETSLLTPGIIEMTGVAGVPDEEVFGPLLRVWRYDSFEEA
                     ILMANNTRFGLSCGLVSPEREKFDQLLLEARAGIVNWNKPLTGAASTAPFGGIGASGN
                     HRPSAWYAADYCAWPMASLESDSLTLPATLNPGLDFSDEVVR"
     gene            16451..17794
                     /gene="astB"
                     /locus_tag="ACHOSC_RS10095"
                     /old_locus_tag="pgaptmp_002019"
     CDS             16451..17794
                     /gene="astB"
                     /locus_tag="ACHOSC_RS10095"
                     /old_locus_tag="pgaptmp_002019"
                     /EC_number="3.5.3.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009015 - N-succinylarginine dihydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0006527 - arginine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-succinylarginine dihydrolase"
                     /protein_id="HGU4547961.1"
                     /translation="MNAWEVNFDGLVGLTHHYAGLSFGNEASTRHRFQVSNPRQAAKQ
                     GLLKMKALADAGFPQAVIPPHERPFIPVLRQLGFSGSDEQVLEKVARQAPHWLSSVSS
                     ASPMWVANAATIAPSADTLDGKVHLTVANLNNKFHRSLEAHVTESLLKAIFNDEEKFS
                     VHSALPQVALLGDEGAANHNRLGGHYGEPGMQLFVYGREEGNDTRPSRYPARQTREAS
                     EAVARLNQVNPQQVIFAQQNPDVIDQGVFHNDVIAVSNRQVLFCHQQAFARQSQLLAN
                     LRARVNGFMAIEVPATQVSVSDAVSTYLFNSQLLSRDDGSMMLVLPQECREHAGVWGY
                     LNELLAADNPISELKVFDLRESMANGGGPACLRLRVVLTEEERRAVNPAVMMNDTLFN
                     ALNDWVDRYYRDRLTAADLADPQLLREGREALDVLSQLLNLGSVYPFQREGGGNG"
     gene            17787..18755
                     /gene="astE"
                     /locus_tag="ACHOSC_RS10100"
                     /old_locus_tag="pgaptmp_002020"
     CDS             17787..18755
                     /gene="astE"
                     /locus_tag="ACHOSC_RS10100"
                     /old_locus_tag="pgaptmp_002020"
                     /EC_number="3.5.1.96"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0009017 - succinylglutamate desuccinylase
                     activity [Evidence IEA];
                     GO_process: GO:0006527 - arginine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinylglutamate desuccinylase"
                     /protein_id="HGU4547962.1"
                     /translation="MDNFLALTLTGKKPVITEREINGVRWRWLGDGVLELTPLTPPQG
                     ALVISAGIHGNETAPVEMLDALLGAISHGEIPLRWRLLVILGNPPALKQGKRYCHSDM
                     NRMFGGRWQLFAESGETCRARELEQCLEDFYDQGKESVRWHLDLHTAIRGSLHPQFGV
                     LPQRDIPWDEKFLTWLGAAGLEALVFHQEPGGTFTHFSARHFGALACTLELGKALPFG
                     QNDLRQFAVTASAIAALLSGESVGIVRTPPLRYRVVSQITRHSPSFEMHMASDTLNFM
                     PFEKGTLLAQDGEERFTVTHDVEYVLFPNPLVALGLRAGLMLEKIS"
BASE COUNT         4420 a         4862 c         5096 g         4423 t
ORIGIN      
        1 gtgttcgcag cgataacatc caacggtttg aatgaaagag gcctgattac cgcgggatgc
       61 tcttttcttt atctattaat tatgatgggg cttattgttc gggccggttt taaagcaaaa
      121 aaagaacaac tgcattatta tcaggctaaa ggtattgagc cgctcagtat cgagaagtta
      181 caggcgctac aattgatcgc accttatcgg ttctatcata agcaatggtc tgaaacgctg
      241 gagttctggc cgcgaaagcc tgaacctggc aaagatacct tccaatatca tgtgcttccc
      301 tttgactcga tcgatatcat aagcaaaaga cgcgagtctt tagaggatca atggggtatc
      361 gaagatagcg aaagttattg tgccttaatg gagtattttc tttctggcga ccatggagcc
      421 aataccttta aagcaaacat ggaggaagcc ccagagcagg ttatcgcctt gttgaataaa
      481 tttgctgttt ttccctcaga ctatatctct gattgcgcta ataatcgctc cggtaaatcc
      541 tcggcgaagc taatatgggc ggcggaatta tcatggatga cctcgatatc aagcacagct
      601 tttcaaaacg ggacaattga agaagaactg gcctggcatt atataatgct tgcttctcga
      661 aaggcgcacg agttgttcga aagcgaagaa gattatcaaa aaaatagtct aatggggttt
      721 ctttactggc atatctgctg ctatcgcaga aagttaacgg atgctgaact tgaagcgtgt
      781 tatcgttacg acaagcagtt ttgggagcac tacagtaaga aatgccgttg gcccataaga
      841 aatgttccgt ggggagcatc atccgttaaa tactcataaa cgcctgaaat tttgccgggg
      901 gcgctgcgct tgcccggcct acattagagc gtaactgcaa tgaattgtgc ccatttgtag
      961 gcctgataag cgtagcgcca tcaggcattt acaacttaaa tcacaccctg cgccagcatc
     1021 gcatcggcaa ccttcacaaa accggcaatg ttcgcgccct gcacgtagtt ggtttgctca
     1081 ccttcaccac catgctcaac acaggcatgg tggatatcca gcatgatgtg atgcaaacgt
     1141 gcgtcaactt tctcggcttt ccagcccagg cgcgcagcgt tttgcgccat ttcaaggcct
     1201 gacgttgcta caccgccagc attagccgct ttacccggtg caaacagtac gcctgcctgc
     1261 tggaacagtt cagtcgcttc gatggtggtc ggcatatttg ccccttcggc gacggcttta
     1321 acgccattgg cgataagctg atgcgcggcg tcaacatcca gttcgttctg ggtggcgcaa
     1381 ggcagggcga tatcaaccgg tacagaccac ggctgttggc gttcgagata gaccagacca
     1441 aattctttgg cataatctgc cactcgacca tcgcggctgg ctttgatttc gataagacgt
     1501 gccagtttct ctttcgtgaa tccgctttca tcaactacag tgccgctgga gtctgacgca
     1561 gtgatcacac gagcaccaaa ttccatcgct ttttcgatag cgtactgggc gacgttgccg
     1621 gagccagaaa cggaaacgcg catcccttca aaacccatac cgtggcgttt tagcattgct
     1681 tctgtgaaat aaaccagacc gtagccggta gcttccgggc gaataagact gccgccaaat
     1741 gaaaggccct taccggtgaa gacgcaggcg gtattgttgg agagcttttt catcatcccc
     1801 gccataaagc cgacttcacg accaccaacc ccgatatcac ctgccggaac gtcggtatcc
     1861 gcgcccaggt ggcgatacag ttcagtcatc agcgcctggc aaaaacgcat cacttcacct
     1921 tcgctttttc ctttcggatc gaaatcgctg ccgcctttac caccgcccat tggcagggta
     1981 gtcagggcat ttttgaaggt ttgttcgaag ccgaggaatt taagaatgga gaggttaact
     2041 gacggatgga agcgcatacc gcctttgtac gggccgatgg cagagctgaa ctgcacacgc
     2101 catgcacggt tgacctgtac ctgattgcga tcatcaaccc ataccacgcg aaactggatc
     2161 acgcgctccg gttcaaccag acgctccagt aatgacatct ggcgatattt tggattttgt
     2221 tcaagaaaag gccagagtgt ggtcattact tcacgaacgg cttgcgcgaa ctcggtttga
     2281 ttcgggtcgc gcttttggac atggttgagg aatgactcca gagaatatgt ctgatccata
     2341 gatataaaac ccttatatat taatacgatt gtgcttatgt tgtttatgtg atgtgctttt
     2401 gcagttttcg actataccat caagaatgtg acggtaagca agaaaaaaat gacccaggaa
     2461 agcaaattaa taacgagagt aatctcataa ccaaatgcga taactcagac gctaaagttt
     2521 tgccacccgg ctaccgttat cagtagcaga gacaataacg ttaaggagtt ccctgtgagt
     2581 cgagcattgt tcgccgtagt attagcattt ccattaattg cactggctaa cccgcattat
     2641 cgtccggacg tggaggtaaa cgtaccgcca gaagtgttca gttcaggcgg gcagtcagcg
     2701 cagccctgta ctcagtgctg cgtttatcag gataaaaatt attctgaagg cgcggtaatt
     2761 aaagccgagg gaatactgct gcaatgccag cgggatgaca aaacccttag cactaatccg
     2821 ctggtctggc ggcgcgtaaa gccataaacg cctctaataa tggaatgtca gcaggggcca
     2881 gcggatattg cagcgcctct tcaggtgagc accagaccag cgcctgatgt tcatgtgcct
     2941 gtaacgtccc gtggaagtcg ggtacgtgcc aggcatgaag atggataatc cggcccgaaa
     3001 cttctcgctg atggctggta acatactcac ccacagttgc ttcgatgccc agttcttcgc
     3061 gtaactcacg caccagcgcc tgccgctggc tttcatccgg ctcgacttta ccaccggcaa
     3121 actcccataa tcccgcctga tcgctctggg cggggcgttg cgcgagtaaa attttgccat
     3181 cacgttcaat gatggcggca acaacttcaa tcattttcat aaattacaca cgggcagata
     3241 agcgaaaaat gccataatat ccgcactgtt gctcaatcag aaaggcttta tatttaagga
     3301 gtgcctgtgc tagaccgcca tcttcatccc cggattaaac cgttgttgca tcagtgcgtg
     3361 cgggttcttg ataaaccggg cattacgcca gatggtttaa cattagtggg atttgccatc
     3421 ggcgtgctgg cactgccttt tctggcgctg ggctggtatc tggcggcgct tgtcgtcatt
     3481 ttgttgaaca ggctgcttga tggtctggac ggtgcgctgg cgcggcgcag agggcttacc
     3541 gacgcgggcg gctttcttga tatttctctc gattttcttt tttacgcgct ggtacctttt
     3601 gggtttatcc tcgccgcacc gcagcaaaac gccctggcag gcggctggct gctgtttgcg
     3661 tttatcggca caggcagcag ttttctcgct tttgctgcgc tggcggcgaa acatcagatt
     3721 gataatcccg gttatgcgca taaatcgttt tattatctgg gcgggttaac cgaaggcaca
     3781 gaaacgatct tactgtttgt gctgggatgt ttattccctg catggtttgc gtggtttgcg
     3841 tggatcttcg gtgcgttgtg ctggatgacg acctttacgc gggtgtggag tgggtatctg
     3901 acgctgaagt cgcaccagcg tcagtaattg caatgttatt tgctactgtc cgggccgcgt
     3961 tcaccggttg ctaccggatt ttttggatcg ctgctccatt cgtaccagcc gccgtcatac
     4021 acggaaacat tcttccaacc catggcgcgt gcgtacataa aggtttcgga cgcgcgccag
     4081 ccggtgccgc agtagaatga aacttgctgc tctggtttga tattccacgc tttccacata
     4141 gcggtaatat catcggcgct gcgcatggtg ccatccgggt tatgaaaatc ttccatatgc
     4201 gtcgagtcgc taccagcgtg tccccaacgt gctccggcta tttcaccttt tggtttaata
     4261 tagctgtaac cgctggtcgt accgataaat tctggccacg aacgaatgct caccagcgat
     4321 gcatcctggc gatgcagcag tccacgggcc tgttccatat cgagcatcaa ctgcggttgt
     4381 gccgggatct tcacgccgaa atccggttcc gctttcactt tcggtggcgt tcctcgctca
     4441 acaggcagtc ccgcgtcgga ccaggtttgc cagccgccat ccagcagacg cacatctttc
     4501 acgcctgcgt agagcataat ttgcgccaca cgcgctccag cgtatacgtc acgcccgtac
     4561 agaatgaccg tggtgtcatg gcgaatgccg tgttttgcca gcatcgcttt tagttgttca
     4621 tcagaaactt tgttccacag cggttcgctt tccacttcgt tggtatcgat gtagtcagca
     4681 ccgggaatat ggctgataag gtaaagctta ggagcgcccc aggccgcttc aatgactttc
     4741 cagtcaccgg caggtttcgc cgtaacctct ttaccttgtt gcaggtcgtg cagccattgc
     4801 ggataaacca gctgctcaaa gtgcggcagt ttttgtagac gggaaggttc gcttagcgcg
     4861 tcactcagaa tggagatatg cgtaaaacct gctttttgca gtcgcgtttt gacggcgtcg
     4921 acatctttgt cattaccgta cagcgccacc ggggcatcgg ctttcaggtt atgttgcttg
     4981 atccacgcgt tgagctgttc ggtgctcatt ttgtcgagcc agctggcaga gaggtttaag
     5041 gcggcaggtt catgaccaga agggccattt aaggtttgtg gccagccgtt ataaaacgcg
     5101 ctggggcgag tatctatcgc tttgccattt tgttgttgaa gctggtcaag tgtaagaggc
     5161 ttcgccagtt cagcggccca cgaagaagcg caagccagcc ctaaagccat tgccagcgcg
     5221 gtcatttgag aaacacgttt catcgaaaat cccgacattg tagttttgag tgcgccattc
     5281 tgaactgacg cattgagaaa aactttgcgt tatcgcagtt tgttgtaatt ttctgaccac
     5341 tgctccatat ccagaacaga actatcagca ggaacatcct ggagatcgtg cgttacctga
     5401 acgacgggga tcgccagttc gcgaacttcg ctgaacaccc actggcgaaa attatcgcgc
     5461 agagccacat caagacggct gaatggctca tccaggagta acgcttttgg ctgagcgaga
     5521 agggcgcgca gcagggccac ccgcgctcgc tgaccgcctg ataatgtcgc agggtcctgg
     5581 tgataagttt cagccagtcc cgctcgatca agcgcgtctt tcacggcatt gcgcctggca
     5641 gtccccttga gcgtcgacgg tagcgccagc agtaaatttt gcccgacgct gaactggtca
     5701 aataacagtg catcctggaa aagaatgcca atctgacgct gtgcggtggg tagcatgtca
     5761 atccgttgct cattgagcca tagctcacct gtacaagaaa actgtccagc cagtgcacca
     5821 atcatccatg aaaacagagt ggattttcca cagccagacg gccccattaa cgtgacaatg
     5881 tcacctttat ccaccgtaaa gttaacgttt gtcagcaagc ggctttctgg taaacgtagc
     5941 gaaacatttt tcacgcagag cattagcgga gtccttgtct gacataacct acccattttg
     6001 cgactaacgc ggtcagggca aaaataataa gcggtaatag cagttgccat aaagcctggg
     6061 cggcgaaaat accattgctg ccgccgctgc ttaatgccac cgcctcagtg gtgagcgtcg
     6121 gaaaacgccc cgcgcccagc cacagcgtcg gcatatactg cgcaatactg actgaaaatc
     6181 ccaccgcgaa ggcaatcagc gtagggcgta gcataagcgg acatttcacg taaaagaaga
     6241 ttttggcccg cgaccagctc agtgtttgcg caatcaaaat taaccgtgaa tcaatgcgct
     6301 gccaggcggg ttgcaggata aacagcatcc acggcattac ccacagcaga tgcccccaga
     6361 ccaccgccgt ccagcttcca tccaggttca gccatagcgc cagcgtgtac tggcccgcca
     6421 ccagtggcag agcgggcaat agagtgggca accagagcca caactggcga cgctgtggcc
     6481 cccattccag ccacagcagt aacaggagca aagcgatgaa tgtcgccacc agccccattg
     6541 tcaggctgtt gatgagtgct tcactattga tcgtcgactg atccgcgagg atcgccagca
     6601 gaaccacaca cagcacaccg gttaagggta aaaaactcgc cagcgtagtg cctggtaata
     6661 aaggtgtggc aggcttgcga atgccatcta cgcggggaat agtgcgccgc cagctgcgcc
     6721 atagcagata gctcagcaaa acgtaggcgg cgagtaacag catcagcagc agactggcga
     6781 gtgcgccttt cgtttgttga tccgcgtcgc cctgagttaa ccactgccag ctaattactg
     6841 ccagcgtcgg tggattacct ggcccaagaa taatcgccac atccacgacc gaaagcgacc
     6901 aggcaacaat cgccaacatc gccattgcca gtgcaggtgc gacggagggc aagagcagcc
     6961 agttcaggca ttgccagcgg ctgtagccca gcgaatccag cacaatgacc tgctgcaaca
     7021 gccgtttttc gctcaatact gccgctaaga tccacagcag aaatgcgctt tctttcactg
     7081 caagggtgag gcccagccca atacccaatt tgtccattag cggagtgaaa tacgggaaat
     7141 agtcataaag cagccctccg tcagcaaaga gcagaagggc gctggtggca aaagccacat
     7201 ggggaatggc gagcagccac ggcagacggg cacacatacg ctgccatttc ggccaaggcc
     7261 acagcgccac aatcaccagc agggcaatca acaacgcccc gaccgccgcg atggttgtcg
     7321 acaccagcgt tgccagtaat gcctgcggta actgtggatc ggcaaataac gcctgccagt
     7381 gtgtcaacga caaagcaggc gatatcagcg tgagagctgc cgggatcagt ggtgcataaa
     7441 tcaccgccac catcgcccac agcaaaaaga ttaatgcgta ccgtaacggt gtagccattc
     7501 ttgttccagc gcatttaccc aacctgcgtg cggttcagcc agtaccggcg gcagatcttg
     7561 cggcattctt gattgcaatg tttcgcgctg cccatcaggc agtttttgcg gatcgagaac
     7621 agaaggatcg ccccagacag cgggatccgc tttacgcagt tgcgcattgg gtgagagcag
     7681 gaagttggca accaccttcg ccgcagcact ggcatttgcg ttagcaggaa tggtgacgaa
     7741 atgcacgttg ccaatcatcc cctggttaaa accaaaactg taactgcttg caggcaaatc
     7801 gccgctggcg attttttgct gggcatgagc agggttaaag gtcagcgaca ggcgcaatgt
     7861 gccggctttc agcagagcat ccatccgcgc gggcgaaggc gggaaatcct ttcctttgcg
     7921 ccataaatag ggatgtagcg cgtcaagata ttgccacaag ggcgcagtga cgcgggcgaa
     7981 agtggcttta tccggggcaa cttttaacgc ggcgggatcg ggcgtcagca taatcagcaa
     8041 ctgttcaaga aacgccgtgc cggtaaagtc cggtgggcgc ggataggtaa ccgtgccggg
     8101 attggcttta gcaaactcca gtaaggcttg cggcgtttgt ggtggctgtg gcgtaacatc
     8161 gcggcgggcg ataaacgtca gttgtgcgcc gccccagggc gattcagccc cttctgtagg
     8221 cactgaaaaa tcttcccgca ccggcagctg tgtgtcgaca tagcgccagt tgggcagagt
     8281 ctccgcccag cccgtttgta ataaattggc ctcttttaag gtgcggaagt tttcgccgtt
     8341 cacccagagc agatccaccg agccgcccgt tttacgtccg gcagcagctt cggtctgaat
     8401 gcgcttcacc gcgtctgcgg catccgccag acgaacaatc ttcaggttta tagcgtaatg
     8461 ggttttcatc tcgccgctaa cccagtcgag atagcggtta attgcggtat cgccgcccca
     8521 ggcgttaaac cagacggttt gccctttggc ctcattttta atttcctgcc agtcactggc
     8581 atgtgttgcc agagaaaaca gcgataacaa tcccagcaac cacccacaat ggcgcatctt
     8641 ctctccttaa gaacgttttt ccggaatacg gcacagcccc tgagcgggaa tgtccatggc
     8701 agaattaaaa cgagccgaca ggttttgcag gccgatcaat gccgtcagtt cggtgagcgc
     8761 ctgagcgtca aaatgcgcag ccagtcgggt gcgcagggca tcatcgaccg ttggcggcgt
     8821 tacgcttgcg gcttcggcgt actccagcgc cagccgttct tcatcgctga agagcgggct
     8881 ttgccgccag tcagccactg ccagcagttt atcggtgctg ccggtacgct cggcgacttt
     8941 catactggtg atatccacgc aaaactcaca caggcacatt tgcgcaatgc gcgcgctgac
     9001 aagcgatcgc actaccggat cgagcggtga gcgtttgcgc tccagccacc caacaaacat
     9061 tgacaccaaa taaaagataa acgggatccg cccccaccag cgaatcgggc ttagcacctc
     9121 gccgtaatgg cgacgatgca gccacgcctg cggacgtaaa atgaaaggaa ttttgctaag
     9181 cggcggtaaa cccatcaggc attcctcttc tggcgctcaa gccatatttt cttcgccata
     9241 aaaatcacca cggtcagggc aaacagaata aacagtccgg tgacaaacca gaaagtaccg
     9301 cctgttaaca tgctgcccgc ccaggaataa acaatagtcg ccggtaattg accaagcccg
     9361 gtggcgataa aaaacgagcg aaaacgtatt gaagtcagac cggcagcata gctgattgga
     9421 tcgaaaggga caaaaggcaa taaccgacag accaggatgg tgtgtttgcc gtagcgagtg
     9481 aaaaagccgt ccatactgtc aagcacggtt ttgccggtta atttttccac cacttcgcgg
     9541 cccatcactc tggcgataaa aaagcacagc gccgcgccgg ccatcgaact ggtccacgac
     9601 agcaagccgc cccagaacgc gccaaacagc gacgcattgg caaaggtgat caaaaacgca
     9661 ggtagcggtg cagcaatggc ctgtaaaatc atcaacaaga acgagacaac tgccgccagt
     9721 gcgccgtaag actgaataaa gcgttctata ccctgttggt ccacggcggc aaacgccgca
     9781 acgctgcgat tgataaactc atggacgccg ggaagcagcg cccatgcgag cagcatagcg
     9841 aacaacagga tgatgagggt tatacggtag taccagattt tacgcgattg tatcttcatc
     9901 atcctttatc ctggctttat tcggtgattt taaaggtaac cgttgccagc gtgttatccg
     9961 ccgggagaac tgaggcattg cctttgaatt tcacttcgcc gcgtttttcc accgcaccat
    10021 aagtgtatgt tgcattgctg acgatgccga ccgggcagct atccagacac accaggcaac
    10081 ccgttttctt ctcttctgct gccgttaaat taccgccaaa gcgcatgtcc agtttcttgc
    10141 cattactgtc aacaatcact tcatcgaagg aatacgcttt tgccgcccct tgccagttga
    10201 ccgaaatatc cagtttgctg cctgtgacat gagtcgtttc tttattatcc atcgtcatgt
    10261 tttcgcccgg cgtaccacct gcctctttca gggcttcata aaatgctttc ggggtcgcat
    10321 aagccataaa cagcgattta tgtccgttgg agccatcttt aaacacaata ccgtgacgag
    10381 tgtcgtcggt gaggaaacga ccattaattt gcaccagcat ggttaccgta tggttattgg
    10441 catcgacgcg cagcggttgg ctgttcgaaa gtccgtcgta ttccactttt gccgccgcat
    10501 tctctttttg atcgcagccg ctaagcccgg caacagccag taagcagagt gcagccagtc
    10561 cttttttcca tgacactgaa taatgttgcg acatcgttct atccttcatt ttttgggtga
    10621 gtaagtgggc tgcggcgcga agcagacaga tccacatgtt tgtgtcgggc gtagagtttt
    10681 gcgagctgga cgagaataaa cagcgccagg cccgccagac agagttgtaa aataaagcgc
    10741 agcgtaatgc cttcattggc gagatcgctt gccatcacgg tataaataac aataccgagc
    10801 agggtcgtga gtgccgaaat aagggtataa ggccagaagg cgatggtggt taatccgtaa
    10861 gcgtaatttt gaatattgta aggaaacaac gggattaagc gggtcagaat aagaaaatca
    10921 ataccgttac gcgcaatgcc tttttcaatg gcctggaagg tatggctatg gcccacgtat
    10981 ttcagcagta aatcacgtcc cagccagcgc gccagcagga atgagcacga ggaggccagc
    11041 gttgcggcaa ttaatgagag tagtgtccct aagagcgggc caaaaactat tcctccagcg
    11101 atcaccagta tgcttcctgg taacagaaag agggtggcaa tgatgaataa cagaatatag
    11161 aggctataac cgaacaatcc gctctggcga atgagtgtct gtaagtgggg taaatcggtg
    11221 agcagatcga ataaaccgaa agcgtggata gcgtaaatga ccagcgcaaa aataagacag
    11281 gcaaacagaa agttacgctc agcgttcatc gtggagtttc ctatatcaaa cttgataata
    11341 gagaagtggt cagacaacta aaaacgcagt gacggtataa caaaggacgg caggcaacaa
    11401 atcattactg acagtttgtt gatttttctc aaggttaatt ctcctgaccc agtttgagcc
    11461 aggagagctg ctaaattagc ggcggaaggt cgcccagacg ggggcgtgat cggacggttt
    11521 ttccatgctg cgaatttcat agtcgatgcc ggtttctacg caacattctg ccagcggttg
    11581 gctggcgagc agcaggtcga tgcgcagacc acggttatcg tcaaaacctt ttgagcggta
    11641 atcaaaccat gagaaacgat ctgctgtttg cggattagca tggcggaagg tatcgaccaa
    11701 cccccagctc atcagcctgt ccatccattc gcgctcttcc ggcaggaaag agcatttacc
    11761 ggtacgcagc cagcgcttac ggttttcttc gccaatgccg atatccagat ctgtagggct
    11821 gatattcata tcgcccataa tcagtaccgg attatcacgt ttgagttcgg tttccaggta
    11881 gttttgcaga ttctgataaa actgcgcttt tgccgggaat tttatcggat ggtcgcggct
    11941 ttcaccctgc gggaagtaac cgttgatcac ggtgacatta cccagcagtg aggggatttc
    12001 cgccataata atccgccgct gcgcctcttc gtcgtcaccg ggaaagccgc gacgcacggc
    12061 aatcggcgtc tctttggtca gcagcgccac gccataatgg cctttctgcc cgtgataaaa
    12121 cacgttgtag ccgagcttcg ccacctcttc gagcggaaac atatcgtcat gaacttttgt
    12181 ctcctgcagg ccaatcacat ccggttggtg cttttcgacg atggcttcaa gctggtgagg
    12241 tctggcgcgc aggccgttga tattaaaaga gacaaatttc atagtcgctg ccatttattc
    12301 agataaagag tgcgtggatg gtagcagaat ttcgcgttgc ttaccgcctg ttgttaagtg
    12361 atttgatcga tggttgttaa tgatgtcaac gatggcgcaa aaaatgcccg cttttggtgc
    12421 gcgctgcgtc agaatggcgc agtaatttcc agtaaatttc gtcaatgatc acaattccag
    12481 aattatattt tacttctgca taacattgcg ttttttattc tttttattca cccacatcgc
    12541 aaacgtattc actttatatg cactttaaat gcatatggtt tggttataac tccttgattt
    12601 ccagttagcc tccgccgttt atgcactttt atcactggct ggcacgaacc ctgcaatcta
    12661 catttacagc gcaaacatta cttattgtta acatataaat aacgaattat ttactgtaga
    12721 ggtcgctatg tctcagccaa ttacgcgtga aaactttgat gaatggatga tacctgttta
    12781 cgctccggca ccctttatac cggtacgtgg cgaaggttcg cgcttgtggg atcagctggg
    12841 gaaagagtat atcgacttcg cgggtggcat tgcggtgaac gcgctgggcc atgcgcatcc
    12901 ggaactgcgt gaagcgctga acgaacaggc gagtaagttc tggcataccg gcaacggtta
    12961 caccaacgag ccggtactgc gactggcgaa aaaattgatc gacgccacgt ttgccgatcg
    13021 cgtcttcttt tgtaactccg gtgcggaagc caacgaagcg gcgctaaaac tggcgcgtaa
    13081 attcgctcac gaccgctacg gcagccataa gagcggcatc gtggcgttca aaaatgcgtt
    13141 tcatggtcgc acgctgttta ctgtcagtgc gggtgggcag ccagcctatt cacaggattt
    13201 tgcgccactg ccggcggata ttcgtcatgc tgcatataac gatattaact ctgccagcgc
    13261 gctgattgac gactctacct gtgcggtgat tgtcgaaccc atccaggggg aaggcggtgt
    13321 ggtgccagcc agcaacgcgt ttttacaagg tctgcgtgaa ttgtgtaacc gccacaatgc
    13381 gctgttgatt tttgatgaag tacaaaccgg cgtcgggcgc accggggaac tgtatgccta
    13441 tatgcactac ggcgtgacgc ctgatctgtt aactaccgcc aaagcgctgg gcggcggttt
    13501 cccggtcggt gcgttgttgg caaccgaaga gtgcgcccgc gtgatgaccg ttggcactca
    13561 tggcaccacc tatggcggta atccgctggc ctcggcggtg gcaggcaaag tgctggagct
    13621 catcaacaca ccagagatgc ttaatggcgt taaacagcgt cacgactggt ttgttgagcg
    13681 tcttaatact attaatcacc gctatggttt gttcagtgaa gttcgcggct taggtttgct
    13741 gattggctgt gtactgaatg ccgattacgc cgggcaagcg aaacagatct ctcaggaagc
    13801 ggcgaaagca ggcgtgatgg tactgattgc gggtggcaac gtggtgcgtt ttgcgcctgc
    13861 gctcaatgtc agcgaagaag aggtgacgac cggactggat cgctttgcag ctgcttgcga
    13921 acactttgtt agccgaggtt catcatgatg gtcatccgtc ccgttgagcg atcagatgtc
    13981 tcggcgctga tgcagcttgc cagcaaaacg ggcggcggcc tgacgtcgct tcccgccaat
    14041 gaagccacgc tttcggcgcg tatcgaaagg gcaatcaaaa cctggcaagg cgaactgccc
    14101 aaaagtgagc agggctatgt gttcgtgctg gaagatagcg agacaggcac cgtggcgggg
    14161 atttgtgcca ttgaggtggc ggttgggctg aacgatccct ggtacaacta tcgcgtcggc
    14221 acgttggttc acgcctcaaa agagctgaat gtctataacg cattgccgac gctgtttctc
    14281 agtaacgatc acaccggcag cagcgagctg tgcacgctgt ttctcgaccc ggactggcgc
    14341 aaagagggca acggctattt gctgtcgaaa tcgcgcttta tgtttatggc ggcttttcgc
    14401 gacaagttta atgacaaagt ggttgctgaa atgcgcgggg tgattgacga acacggctat
    14461 tcaccgttct ggcaaagcct cggtaaacgc ttcttttcga tggattttag ccgcgccgat
    14521 tttctctgcg gcaccgggca aaaggcattt attgcagaac tgatgccgaa acatccgatc
    14581 tatacccact ttttatccca ggaagcccag gacgtcatcg gtcaggtaca tccgcaaacc
    14641 gcgcctgccc gcgcggtgct ggagaaagaa ggttttcgtt accgcaacta tatcgacatc
    14701 ttcgacggcg ggccaacgct tgagtgtgac atcgaccgcg tgcgcgccat tcgtaaaagt
    14761 cggctggtgg aagtggcaga agggcagcct gcgcagggcg attttccggc ctgcctggtc
    14821 gccaatgaaa attatcacca tttccgcgtg gtgctggcgc gtaccgatcc ggcaaccgag
    14881 cgtttgattt taaccgccgc acaactggat gccctcaaat gccacgcagg ggatcgcgtt
    14941 cgtctggtgc gcctgtgcgc agaggagaaa acagcatgac tttatggatt aacggtgact
    15001 ggataacggg ccagggcgca tcgcgtgtga agcgtaatcc ggtatcgggc gaggtgttat
    15061 ggcaaggcaa tgatgccgat gccgctcagg tcgaacaggc ttgtcgggca gcccgtgcgg
    15121 cgtttccgcg ctgggcgcgg ctctcattgg ctgaacgtca ggtcgttgtc gaacgctttg
    15181 ccggattgct ggaaaggaat aaaggcgaat taaccgcgat tattgccaga gaaacgggta
    15241 agccgcgctg ggaagcggca accgaagtga cggcgatgat caataaaatc gcgatatcaa
    15301 ttaaggcgta tcacgttcgt accggcgagc agcgtagtga aatgccggac ggtgcggcga
    15361 gcctgcgaca tcgcccgcac ggcgtgctgg cggtgtttgg gccgtataat ttccctggtc
    15421 atttgccgaa cggacatatc gttccggcat tgctggcagg taacaccatt atctttaaac
    15481 ccagcgaact gacaccgtgg agtggcgaag cggtaatgcg tttatggcag caggctggct
    15541 tgccgccggg cgtactgaac ctggtgcagg gcgggcgtga aacgggtcag gcgctgagtg
    15601 cgctggagga tctcgacggt ttgctgttta ccggtagcgc caataccggc taccagctgc
    15661 atcgccagct ctccggtcag ccggagaaaa ttctcgccct tgagatgggc ggtaataatc
    15721 cgctaattat cgatgaggtg gcggatatcg acgcggctgt ccatctgacc attcagtcgg
    15781 cgtttgtcac agccgggcaa cgctgcacct gcgcccgccg tttattgctc aaaagcggag
    15841 cgcagggcga tgcgtttctt gctcgtctgg ttgccgtcag ccagcgatta acgccgggca
    15901 actgggatga cgaaccgcag ccgtttattg gcgggctgat ttctgaacag gccgcacagc
    15961 aggtggttac tgcctggcag caactggaag cgatgggcgg acgaaccctg cttgcgccgc
    16021 gcttattaca atcagagaca tcgttgctga cgccggggat cattgaaatg acaggcgttg
    16081 ctggcgtacc agatgaagag gtgttcggac cgttattgcg cgtctggcgt tatgattctt
    16141 tcgaggaagc gattctaatg gcgaataaca ctcgcttcgg actctcttgc ggtctggttt
    16201 cccccgagcg ggaaaaattc gatcaactgt tgctggaggc gcgggcgggg attgttaact
    16261 ggaacaaacc gcttactggt gctgccagta ccgcgccatt cggcggcatt ggtgcatccg
    16321 gtaaccatcg ccccagcgcc tggtatgccg cagattactg cgcatggcct atggcgagcc
    16381 tggagtcgga ctcgttaact ttgcccgcaa cgcttaaccc cgggctggat ttttccgatg
    16441 aggtggtgcg atgaacgcct gggaagttaa tttcgacggg ctggtagggc tgacgcatca
    16501 ttacgctggc ctgtcgtttg gtaatgaagc ctctacccgt caccgttttc aggtgtctaa
    16561 cccgcgacag gcggcaaaac agggcttact gaaaatgaaa gcccttgccg atgcgggatt
    16621 cccccaggcc gtgatcccgc cgcacgagcg tccgtttatt ccggtgctgc gtcagttggg
    16681 attcagtggt agcgatgagc aggtactgga aaaagttgca cgccaggcac cgcactggct
    16741 ttccagcgtc agttccgctt cgccaatgtg ggtagccaat gcggcaacga tcgcgccatc
    16801 tgccgatacg ctggatggca aagtgcatct caccgttgcc aacctgaaca ataaatttca
    16861 ccgctcgctg gaagcgcacg tcaccgaatc acttttaaaa gcgattttta acgacgaaga
    16921 gaaatttagc gttcattcgg cgttgccgca ggtagcgttg ctcggtgatg agggggcggc
    16981 aaaccacaat cgtctcggcg gtcattacgg tgagccgggt atgcaacttt ttgtctacgg
    17041 gcgagaagaa ggcaatgata cccggccttc ccgttatccg gcgcgacaga ctcgcgaagc
    17101 cagcgaggcg gtggcaaggc tgaatcaggt gaatccccaa caggtgattt tcgcccagca
    17161 aaacccggac gttatcgacc agggcgtttt tcataatgac gtgattgccg tgagtaaccg
    17221 ccaggtgctg ttttgccacc aacaggcgtt cgctcgccag tcacagttac tggcaaacct
    17281 gcgtgcgcgg gtgaacggtt ttatggcgat agaagttccg gcaactcagg tttccgtgtc
    17341 ggatgcggtg tctacctatc tgtttaacag ccaactgttg agccgcgatg atggttctat
    17401 gatgttggtg ctgcctcagg agtgtcggga acacgccgga gtatggggtt atctcaatga
    17461 actccttgcc gctgacaacc cgattagcga actaaaagtc tttgatttac gtgaaagcat
    17521 ggcgaatggc ggcggcccgg cgtgcctgcg gttgcgggtg gtattgacag aagaagaacg
    17581 ccgggcggtg aatccggcgg tgatgatgaa cgatacgctg tttaatgcgc tcaatgactg
    17641 ggtggatcgt tactaccgcg atcgccttac tgctgccgat ctggccgacc cgcaattgct
    17701 gcgcgaaggg cgggaagcac tggatgtatt gagccaatta ctgaatctcg gttcggttta
    17761 tccgttccag cgcgagggag ggggcaatgg ataattttct tgctctgacc ttaacgggta
    17821 aaaaaccggt tatcaccgag cgagaaatca acggcgttcg ctggcgctgg ctgggcgatg
    17881 gtgtgctgga actgacgcca ttaacgccac cgcaaggcgc actggtgatt tcagcgggaa
    17941 tacacggtaa tgagacggca cctgtggaga tgctggacgc gttgcttggc gcgatatctc
    18001 acggcgagat cccgttacgt tggcggttgc tggtgatcct cgggaatcct cctgcgctga
    18061 agcaagggaa acgttattgc catagcgata tgaatcgaat gtttggcggt cgttggcagc
    18121 tatttgctga aagcggagaa acctgtcggg cgcgcgaact ggaacagtgc ctggaagatt
    18181 tttatgacca gggcaaagaa tctgtgcgct ggcaccttga tctacatacc gcaattcgtg
    18241 gctccttgca tccgcagttc ggtgtattac cgcaacgcga cattccctgg gacgagaaat
    18301 ttctgacgtg gctgggtgcg gcggggctgg aggcgctggt gttccatcag gaacctggtg
    18361 gtacgtttac ccatttcagc gccagacatt ttggcgcgct ggcctgtacg ctggaacttg
    18421 gcaaagcgtt gccctttggg caaaacgatc ttcgccagtt tgcagtaact gccagcgcaa
    18481 ttgctgcgct gctatctggt gagagtgtcg gtatcgtgag aacaccgccg ctccgttatc
    18541 gggtggtttc gcaaattact cgccactcgc cgtccttcga aatgcatatg gcaagtgaca
    18601 cgctgaattt tatgccgttt gagaaaggaa cattgctggc gcaggacgga gaggaacgtt
    18661 ttaccgtaac ccatgatgta gagtatgtgt tattccctaa tccgttggta gcgttgggat
    18721 tacgcgcggg attaatgctc gaaaaaataa gctaatactt acccgcagaa atcactctgc
    18781 gggtattaag ttgctttctt a
//
LOCUS       DAZKHF020000070        18661 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2201S31241
            SAMN43267800-rid25106413.denovo.070, whole genome shotgun sequence.
ACCESSION   DAZKHF020000070 DAZKHF020000000
VERSION     DAZKHF020000070.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267800
            Sequence Read Archive: SRR30307263
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 18661)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18661)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 18661)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350581.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350581.2
            Genome Coverage       :: 151x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:21:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,652
            CDSs (total)                      :: 5,557
            Genes (coding)                    :: 5,320
            CDSs (with protein)               :: 5,320
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 237
            CDSs (without protein)            :: 237
            Pseudo Genes (ambiguous residues) :: 0 of 237
            Pseudo Genes (frameshifted)       :: 102 of 237
            Pseudo Genes (incomplete)         :: 130 of 237
            Pseudo Genes (internal stop)      :: 52 of 237
            Pseudo Genes (multiple problems)  :: 41 of 237
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18661
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267800-rid25106413.denovo.070"
                     /serotype="O 26:H11"
                     /isolate="2201S31241"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-01"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            172..477
                     /locus_tag="ACHOSC_RS10105"
                     /old_locus_tag="pgaptmp_002021"
     CDS             172..477
                     /locus_tag="ACHOSC_RS10105"
                     /old_locus_tag="pgaptmp_002021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415507.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine-rich inner membrane protein GfcA"
                     /protein_id="HGU4547963.1"
                     /translation="MKHKLSAILMAFMLTTPAAFAAPEATNGTEATTGTTGTTTTTTG
                     ATTTATTTGGVAAGAVGTATVVGVATAVGVATLAVVAANDSGDGGSHNTSTTTSTTR"
     gene            584..1228
                     /gene="gfcB"
                     /locus_tag="ACHOSC_RS10110"
                     /old_locus_tag="pgaptmp_002022"
     CDS             584..1228
                     /gene="gfcB"
                     /locus_tag="ACHOSC_RS10110"
                     /old_locus_tag="pgaptmp_002022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein GfcB"
                     /protein_id="HGU4547964.1"
                     /translation="MRPLILSIFALFLAGCTHSQQSMVDTFRASLFDNQDITVADQQI
                     QALPYSTMYLRLNEGQRIFVVLGYIEQEQSKWLSQDNAMLVTHNGRLLKTVKLNNNLL
                     EVTNSGQDPLRNALAIKDGSRWTRDILWSEDNHFRSATLSSTFSFAGLETLNIAGRNV
                     LCNVWQEEVTSTRPEKQWQNTFWVDSATGQVRQSRQMLGAGVIPVEMTFLKPAP"
     gene            1225..1971
                     /locus_tag="ACHOSC_RS10115"
                     /old_locus_tag="pgaptmp_002023"
     CDS             1225..1971
                     /locus_tag="ACHOSC_RS10115"
                     /old_locus_tag="pgaptmp_002023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="capsule biosynthesis GfcC D2 domain-containing
                     protein"
                     /protein_id="HGU4547965.1"
                     /translation="MNKLQSYFIASVLYVMTPHAFAQGTVTIYLPGEQQTLSVGPMEN
                     VVQLVTQPQLRDRLWWPGALLTDSAAKAKALKDYQHVMAQLASWEAEADDDVAATIKS
                     VRQQLLNLNITGRLPVKLDPDFVRVDENSNPPLVGDYTLYTVQRPVTITLLGAVSGAG
                     QLPWQAGRSVTDYLQDHPRLAGADKNNVMVITPEGETVVAPVALWNKRHVEPPPGSQL
                     WLGFSAHVLPEKYADLNDQIVSVLTQRVPD"
     gene            1971..4067
                     /locus_tag="ACHOSC_RS10120"
                     /old_locus_tag="pgaptmp_002024"
     CDS             1971..4067
                     /locus_tag="ACHOSC_RS10120"
                     /old_locus_tag="pgaptmp_002024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjbH domain-containing protein"
                     /protein_id="HGU4547966.1"
                     /translation="MKKNSYLLSCLAIAVSSACHAEVLTYPDPLGSSQSDFGGTGLLQ
                     MPNARIAPEGEFSVNYRDNDQYRFYSTSVALFPWLEGTIRYTDVRTRKYSQWEDFSGD
                     QSYKDKSFDFKLRLWEEGYWLPQVAFGKRDIAGTGLFDGEYLVASKQAGPFDFTLGMA
                     WGYAGNAGNITNPFCRVSDKYCHRAESHDAGDISFSDIFRGPASIFGGIEYQTPWNPL
                     RLKLEYDGNNYQNDFAGKLPQASHFNVGAVYRAASWADLNLSYERGNTLMFGFTLRTN
                     FNDLRPALRDTPKPAYQPAPESEGLQYTTVANQLTALKYNAGFEAPEIQLRDKTLYMS
                     GQQYKYRDSREAVDRANRILVNNLPQGVEKISVTQKREHMAMVTTETDVASLRKQLAG
                     TAPGQSEQLQQQRVEAEDLSAFGRGYRIREDRFSYSFNPTLSQSLGGPEDFYMFQLGL
                     MSSARYWFTDHLLLDGGIFTNIYNNYDKFKSSLLPADSTLPRVRTHIRDYVRNDVYLN
                     NLQANYFADLGNGFYGQVYGGYLETMYAGVGSELLYRPLDASWALGVDVNYVKQRDWD
                     NMMRFTDYSTPTGFVTAYWNPPTLNGVLMKLSVGQYLAKDKGATIDVAKRFDSGVAVG
                     VWAAISNVSKDDYGEGGFSKGFYISIPFDLMTIGPNRNRAVVSWTPLTRDGGQMLSRK
                     YQLYPMTAEREVPVGQ"
     gene            4113..5252
                     /locus_tag="ACHOSC_RS10125"
                     /old_locus_tag="pgaptmp_002025"
     CDS             4113..5252
                     /locus_tag="ACHOSC_RS10125"
                     /old_locus_tag="pgaptmp_002025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0015774 - polysaccharide transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide export protein"
                     /protein_id="HGU4547967.1"
                     /translation="MKKNIFKFSVLTLAVLSLTACTLVPGQNLSTSNKDVIELPDNQY
                     DLDKMVNIYPVTPGLIDQLRAKPIMSQANPELEQQIANYEYRIGIGDVLMVTVWDHPE
                     LTTPAGQYRSASDTGNWVNADGAIFYPYIGRLKVAGKTLTQVRNEITARLDSVIESPQ
                     VDVSVAAFRSQKAYVTGEVSKSGQQPITNIPLTIMDAINAAGGLTADADWRNVVLTQN
                     GVKTKVNLYALMQRGDLRQNKLLHPGDILFIPRNDDLKVFVMGEVGKQSTLKMDRSGM
                     TLAEALGNAEGMNQDVADATGIFVIRATQNKQNGKIANIYQLNAKDASAMILGTEFQL
                     EPYDIVYVTTAPLARWNRVISLLVPTISGVHDLTETSRWIQTWPN"
     gene            5240..5686
                     /gene="etp"
                     /locus_tag="ACHOSC_RS10130"
                     /old_locus_tag="pgaptmp_002026"
                     /gene_synonym="yccY"
     CDS             5240..5686
                     /gene="etp"
                     /locus_tag="ACHOSC_RS10130"
                     /old_locus_tag="pgaptmp_002026"
                     /gene_synonym="yccY"
                     /EC_number="3.1.3.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309165.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-tyrosine-phosphatase Etp"
                     /protein_id="HGU4547968.1"
                     /translation="MAQLKFNSILVVCTGNICRSPIGERLLRKRLPGVKVKSAGVHGL
                     VKHPADATAADVAANHGVSLEGHAGRKLTAEMARNYDLILAMESEHIAQVTAIAPEVR
                     GKTMLFGQWLEQKEIPDPYRKSQDAFEHVYGMLERASQEWAKRLSR"
     gene            5706..7886
                     /gene="etk"
                     /locus_tag="ACHOSC_RS10135"
                     /old_locus_tag="pgaptmp_002027"
     CDS             5706..7886
                     /gene="etk"
                     /locus_tag="ACHOSC_RS10135"
                     /old_locus_tag="pgaptmp_002027"
                     /EC_number="2.7.10.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-protein kinase"
                     /protein_id="HGU4547969.1"
                     /translation="MTTKNMNTPPGSTQENEIDLLRLVGELWDHRKFIISVTALFTLI
                     AVAYSLLSTPIYQADTLVQVEQKQGNAILSGLSDMIPNSSPESAPEIQLLQSRMILGK
                     TIAELNLRDIVEQKYFPIVGRGWARLTKEKPGELAISWMHIPQLNGQDQQLTLTVGEN
                     GHYTLEGEEFTVNGMVGQRLEKDGVALTIADIKAKPGTQFVLSQRTELEAINALQETF
                     TVSERSKESGMLELTMTGDDPQLITRILNSIANNYLQQNIARQAAQDSQSLEFLQRQL
                     PEVRSELDQAEEKLNVYRQQRDSVDLNLEAKAVLEQIVNVDNQLNELTFREAEISQLY
                     KKDHPTYRALLEKRQTLEQERKRLNKRVSAMPSTQQEVLRLSRDVEAGRAVYLQLLNR
                     QQELSISKSSAIGNVRIIDPAVTQPQPVKPKKALNVVLGFILGLFISVGAVLARAMLR
                     RGVEAPEQLEEHGISVYATIPMSEWLDKRTRLRKKNLFSNQQRHRTKNIPFLAVDNPA
                     DSAVEAVRALRTSLHFAMMETENNILMITGATPDSGKTFVSSTLAAVIAQSDQKVLFI
                     DADLRRGYSHNLFTVSNEHGLSEYLAGKDELNKVIQHFGKGGFDVITRGQVPPNPSEL
                     LMRDRMRQLLEWANDHYDLVIVDTPPMLAVSDAAVVGRSVGTSLLVARFGLNTAKEVS
                     LSMQRLEQAGVNIKGAILNGVIKRASTAYSYGYNYYGYSYSEKE"
     gene            complement(8001..9299)
                     /gene="appA"
                     /locus_tag="ACHOSC_RS10140"
                     /old_locus_tag="pgaptmp_002028"
     CDS             complement(8001..9299)
                     /gene="appA"
                     /locus_tag="ACHOSC_RS10140"
                     /old_locus_tag="pgaptmp_002028"
                     /EC_number="3.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional acid phosphatase/4-phytase"
                     /protein_id="HGU4547970.1"
                     /translation="MKAILIPFLSLLIPLTPQSAFAQSEPELKLESVVIVSRHGVRAP
                     TKATQLMQDVTPDAWPTWPVKLGWLTPRGGELIAYLGHYQRQRLVADGLLAKKGCPQS
                     GQVAIIADVDERTRKTGEAFAAGLAPDCAITVHTQADTSSPDPLFNPLKTGVCQLDNA
                     NVTDAILSRAGGSIADFTGHRQTAFRELERVLNFPQSNLCLKREKQDESCSLTQALPS
                     ELKVSADNVSLTGAVSLASMLTEIFLLQQAQGMPEPGWGRITDSHQWNTLLSLHNAQF
                     YLLQRTPEVARSRATPLLDLIKTALTPHPPQKQAYGVTLPTSVLFIAGHDTNLANLGG
                     ALELNWTLPGQPDNTPPGGELVFERWRRLSDNSQWIQVSLVFQTLQQMRDKTPLSLNT
                     PPGEVKLTLAGCEERNAQGMCSLAGFTQIVNEARIPACSL"
     gene            complement(9379..9471)
                     /gene="cbdX"
                     /locus_tag="ACHOSC_RS10145"
                     /old_locus_tag="pgaptmp_002029"
     CDS             complement(9379..9471)
                     /gene="cbdX"
                     /locus_tag="ACHOSC_RS10145"
                     /old_locus_tag="pgaptmp_002029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome bd-II oxidase subunit CbdX"
                     /protein_id="HGU4547971.1"
                     /translation="MWYLLWFVGILLMCSLSTLVLVWLDPRLKS"
     gene            complement(9484..10620)
                     /gene="appB"
                     /locus_tag="ACHOSC_RS10150"
                     /old_locus_tag="pgaptmp_002030"
     CDS             complement(9484..10620)
                     /gene="appB"
                     /locus_tag="ACHOSC_RS10150"
                     /old_locus_tag="pgaptmp_002030"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome d ubiquinol oxidase subunit II"
                     /protein_id="HGU4547972.1"
                     /translation="MFDYETLRFIWWLLIGVILVVFMISDGFDMGIGCLLPLVARNDD
                     ERRIVINSVGAHWEGNQVWLILAGGALFAAWPRVYAAAFSGFYVAMILVLCSLFFRPL
                     AFDYRGKIADARWRKMWDAGLVIGSLVPPVVFGIAFGNLLLGVPFAFTPQLRVEYLGS
                     FWQLLTPFPLLCGLLSLGMVILQGGIWLQLKTVGVIHLRSQLATKRAALLVMLCFLLA
                     GYWLWVGIDGFVLLAQDANGPSNPLMKLVAVLPSAWMNNFVESPVLWIFPLLGFFCPL
                     LTVMAIYRGRPGWGFLMASLMQFGVIFTAGITLFPFVMPSSVSPISSLTLWDSTSSQL
                     TLSIMLVIVLIFLPIVLLYTLWSYYKMWGRMTTETLRRNENELY"
     gene            complement(10632..12177)
                     /gene="appC"
                     /locus_tag="ACHOSC_RS10155"
                     /old_locus_tag="pgaptmp_002031"
                     /pseudo
     CDS             complement(10632..12177)
                     /gene="appC"
                     /locus_tag="ACHOSC_RS10155"
                     /old_locus_tag="pgaptmp_002031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706901.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome bd-II oxidase subunit 1"
     gene            complement(12311..13168)
                     /locus_tag="ACHOSC_RS10160"
                     /old_locus_tag="pgaptmp_002032"
     CDS             complement(12311..13168)
                     /locus_tag="ACHOSC_RS10160"
                     /old_locus_tag="pgaptmp_002032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase expression/formation protein"
                     /protein_id="HGU4547973.1"
                     /translation="MSETFFHLLGPGTQPNDDSFSMNPLPITCQVNDEPSMAALEQCA
                     HSPQVIALLNELQHQLSERQPPLGEVLAVDLLNLNADDRHFINTLLGEGEVSVRIQQA
                     DDSESEIQEAIFCGLWRVRRRRGEKLLEDKLEAGCAPLALWQAATQNLLPTDSLLPPP
                     IDGLMNGLPLAHELLAHVRNPDAQPHSINLTQLPISEADRLFLSRLCGPGNIQIRTIG
                     YGESYINATGLRHVWHLRCTDTLKGPLLESYEICPIPEVVLAAPEDLVDSAQRLSEVC
                     QWLAEAAPT"
     gene            complement(13165..13563)
                     /gene="hyaE"
                     /locus_tag="ACHOSC_RS10165"
                     /old_locus_tag="pgaptmp_002033"
     CDS             complement(13165..13563)
                     /gene="hyaE"
                     /locus_tag="ACHOSC_RS10165"
                     /old_locus_tag="pgaptmp_002033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase-1 operon protein HyaE"
                     /protein_id="HGU4547974.1"
                     /translation="MSNDTPFDALWQRMLARGWTPVSESRLDDWLTQAPDGVLLLSSD
                     PKRTPEVSDNPVMIGELLREFPDYTWQVAIADLEQSEAIGDRFGVFRFPATLVFTGGN
                     YRGVLNGIHPWAELINLMRGLVEPQQERAS"
     gene            complement(13560..14147)
                     /gene="hyaD"
                     /locus_tag="ACHOSC_RS10170"
                     /old_locus_tag="pgaptmp_002034"
     CDS             complement(13560..14147)
                     /gene="hyaD"
                     /locus_tag="ACHOSC_RS10170"
                     /old_locus_tag="pgaptmp_002034"
                     /EC_number="3.4.23.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 1 maturation protease"
                     /protein_id="HGU4547975.1"
                     /translation="MSEQRVVVMGLGNLLWADEGFGVRVAERLYAHYHWPEYVEIVDG
                     GTQGLNLLGYVESASHLLILDAIDYGLEPGTLRTYAGERIPAYLSAKKMSLHQNSFSE
                     VLALADIRGHLPAHIALVGLQPAMLDDYGGSLSELAREQLPAAEQAALAQLAAWGIVP
                     QPANESRCLNYDCLSMENYEGVRLRQYRMTQEEQG"
     gene            complement(14144..14851)
                     /gene="hyaC"
                     /locus_tag="ACHOSC_RS10175"
                     /old_locus_tag="pgaptmp_002035"
     CDS             complement(14144..14851)
                     /gene="hyaC"
                     /locus_tag="ACHOSC_RS10175"
                     /old_locus_tag="pgaptmp_002035"
                     /EC_number="1.12.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ni/Fe-hydrogenase b-type cytochrome subunit"
                     /protein_id="HGU4547976.1"
                     /translation="MQQKSDNVVSHYVFEAPVRIWHWLTVLCMAVLMVTGYFIGKPLP
                     SVSGEATYLFYMGYIRLIHFSAGMVFTVVLLMRIYWAFVGNRYSRELFIVPVWRKSWW
                     QGVWYEIRWYLFLAKRPSADIGHNPIAQAAMFGYFLMSVFMIITGFALYSEHSQYAIF
                     APFRYVVEFFYWMGGNSMDIHSWHRLGMWLIGAFVIGHVYMALREDIMSDDTVISTMV
                     NGYRSHKFGKISNKERS"
     gene            complement(14870..16663)
                     /gene="hyaB"
                     /locus_tag="ACHOSC_RS10180"
                     /old_locus_tag="pgaptmp_002036"
     CDS             complement(14870..16663)
                     /gene="hyaB"
                     /locus_tag="ACHOSC_RS10180"
                     /old_locus_tag="pgaptmp_002036"
                     /EC_number="1.12.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ni/Fe-hydrogenase large subunit"
                     /protein_id="HGU4547977.1"
                     /translation="MSTQYETQGYTINNAGRRLVVDPITRIEGHMRCEVNINDQNVIT
                     NAVSCGTMFRGLEIILQGRDPRDAWAFVERICGVCTGVHALASVYAIEDAIGIKVPDN
                     ANIIRNIMLATLWCHDHLVHFYQLAGMDWIDVLDALKADPRKTSELAQSLSSWPKSSP
                     GYFFDVQNRLKKFVEGGQLGIFRNGYWGHPQYKLPPEANLMGFAHYLEALDFQREIVK
                     IHAVFGGKNPHPNWIVGGMPCAINIDESGAVGAVNMERLNLVQSIITRTADFINNVMI
                     PDALAIGQFNKPWSEIGTGLSDKCVLSYGAFPDIANDFGEKSLLMPGGAVINGDFNNV
                     LPVDLVDPQQVQEFVDHAWYRYPNDQVGRHPFDGITDPWYNPGDVKGSDTNIQQLNEQ
                     ERYSWIKAPRWRGNAMEVGPLARTLIAYHKGDAATVESVDRMMSALNLPLSGIQSTLG
                     RILCRAHEAQWAAGKLQYFFDKLMTNLKNGNLATASTEKWEPATWPTECRGVGFTEAP
                     RGALGHWAAIRDGKIDLYQCVVPTTWNASPRDPKGQIGAYEAALMNTKMAIPEQPLEI
                     LRTLHSFDPCLACSTHVLGDDGSELISVQVR"
     gene            complement(16660..17778)
                     /gene="hyaA"
                     /locus_tag="ACHOSC_RS10185"
                     /old_locus_tag="pgaptmp_002037"
     CDS             complement(16660..17778)
                     /gene="hyaA"
                     /locus_tag="ACHOSC_RS10185"
                     /old_locus_tag="pgaptmp_002037"
                     /EC_number="1.12.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 1 small subunit"
                     /protein_id="HGU4547978.1"
                     /translation="MNNEETFYQAMRRQGVTRRSFLKYCSLAATSLGLGAGMAPKIAW
                     ALENKPRIPVVWIHGLECTCCTESFIRSAHPLAKDVILSLISLDYDDTLMAAAGTQAE
                     EVFEDIITQYNGKYILAVEGNPPLGEQGMFCISSGRPFIEKLKRAAAGASAIIAWGTC
                     ASWGCVQAARPNPTQATPIDKVITDKPIIKVPGCPPIPDVMSAIITYMVTFDRLPDVD
                     RMGRPLMFYGQRIHDKCYRRAHFDAGEFVQSWDDDAARKGYCLYKMGCKGPTTYNACS
                     STRWNDGVSFPIQSGHGCLGCAENGFWDRGSFYSRVVDIPQMGTHSTADTVGLTALGV
                     VAAAVGVHAVASAVDQRRRHNQQPTETEHQPGNEDKQA"
     gene            18205..18292
                     /locus_tag="ACHOSC_RS10190"
                     /old_locus_tag="pgaptmp_002038"
     tRNA            18205..18292
                     /locus_tag="ACHOSC_RS10190"
                     /old_locus_tag="pgaptmp_002038"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:18239..18241,aa:Ser,seq:tga)
BASE COUNT         4558 a         4960 c         4845 g         4298 t
ORIGIN      
        1 cagaggggga tagttaacaa tcttatattt ccctctgcgc gcctttccta cccgcagtat
       61 atagtgcaag cggtatgagg aaagggagat ttcacaagga tgttatactc aaagtagaca
      121 atcctgatct caagcgtacg tattgtccat gcgatgaata aaggaaaagt tatgaaacac
      181 aagctttctg caatccttat ggccttcatg ttgacgacgc cagctgcgtt tgctgcccct
      241 gaagcaacaa acgggaccga ggcaacgacg ggtactactg gcaccacaac gaccactacc
      301 ggcgcaacca cgactgctac taccactggt ggcgtggctg ctggcgctgt cggcacggcg
      361 acagttgtcg gtgtggcaac ggctgttggt gtggcgacgt tggcagttgt tgcagcaaat
      421 gacagtggcg atggcggttc tcataacacc tcaaccacca cgtccactac acgctaatac
      481 tgctttaacg cgtcgaaatt aaagttaaat gatacccggt tgcgatattc gtaccgggct
      541 ttagagattt gcattttttt aattaacctt tcaaggatta aaggtgcgcc ctcttatttt
      601 atcgattttc gcactatttc ttgcgggatg tacgcacagc cagcaaagta tggtcgatac
      661 atttcgcgcc agccttttcg ataatcagga tatcaccgta gcggatcagc agatccaggc
      721 gttgccttat tccacgatgt atttacgcct taatgaaggg caacgaatct ttgtggtact
      781 gggatatata gaacaagaac aaagcaaatg gttatcccag gataacgcca tgctggttac
      841 ccacaatgga cgtcttttaa aaaccgtcaa acttaataat aatctgctgg aagtgacaaa
      901 ttccgggcag gaccctctgc gtaacgcgct ggcaataaaa gatggcagcc gctggacgcg
      961 cgatattctc tggagtgaag acaaccattt tcgctctgcg accctgagtt ctactttttc
     1021 ctttgctgga ttagagacgc tgaatattgc gggtcgcaat gtgctgtgta atgtctggca
     1081 ggaagaggtg acttccacgc ggccagaaaa acagtggcaa aacacattct gggtcgattc
     1141 ggctactggc caggttcgtc aaagtcggca aatgttaggc gcaggggtta ttcccgtaga
     1201 aatgacgttt cttaaacccg caccatgaat aaattacagt cgtatttcat tgccagcgta
     1261 ctttacgtaa tgacacccca tgcctttgcg caaggaacgg tgactattta tctgcctggc
     1321 gaacaacaga cactttccgt tggccccatg gaaaatgttg tccagttggt gacacaaccg
     1381 caactgcgcg atcgcctctg gtggcctggt gccttgctga ccgactctgc ggcgaaagct
     1441 aaagcgctta aagactacca acatgtcatg gcacagcttg cttcctggga agcagaagct
     1501 gacgacgatg tggcggcaac gattaaatcc gtgcgccagc agttactcaa tctcaatatc
     1561 actggtcgct taccggtcaa actggaccct gattttgtac gagtggatga gaacagcaat
     1621 cccccgttgg tcggggacta tacgctctat accgtacagc gtccggtgac tattactctg
     1681 ctgggtgcgg tttctggggc cgggcaattg ccctggcagg caggccgtag cgttaccgat
     1741 tatctgcaag atcacccccg ccttgcaggc gcggacaaga ataacgttat ggtgattacg
     1801 cctgagggtg agactgtcgt cgcccctgtt gccctgtgga ataaaagaca tgtggaacca
     1861 ccgcctggca gccagctttg gttgggcttt tctgcgcatg tactgcccga aaaatatgct
     1921 gatttaaatg accagattgt gtctgtttta acgcaacgtg ttcctgactg atgaagaaga
     1981 attcttatct tttaagctgc ctggccattg ccgtctccag tgcctgtcat gctgaagtat
     2041 taacctaccc ggatccgctg ggttcgtcgc aatcagactt tggcggcaca ggattgttgc
     2101 agatgccaaa tgcgcgcatc gcaccggaag gtgaattcag cgtcaactac cgggataacg
     2161 atcaataccg gttctactcc acctccgtgg cgctgttccc atggctggaa ggcaccattc
     2221 gttatacgga tgtgcgcaca cgcaaatata gccagtggga agatttcagc ggcgatcagt
     2281 catacaaaga caaatcattc gattttaaac ttcgcctgtg ggaagaaggt tactggctac
     2341 cgcaagtggc gtttggtaaa cgtgatattg ctggtacggg tctgtttgac ggtgagtatc
     2401 tggtggccag caagcaagcg gggccatttg atttcaccct cgggatggca tggggctacg
     2461 ccggtaatgc gggcaatatt accaacccgt tttgccgggt gagcgataaa tattgtcatc
     2521 gcgcagagtc tcacgatgcg ggcgatatca gctttagcga tatctttcgt ggcccggctt
     2581 ccatctttgg cggcattgag tatcaaacgc cgtggaatcc cctgcgtctg aaactcgaat
     2641 acgatggcaa caattaccag aatgatttcg ctggcaaact gcctcaggca agccatttca
     2701 acgtcggcgc agtttatcgc gctgccagct gggcagatct caacctgagt tatgaacgcg
     2761 gtaacacgtt gatgtttggc ttcacgttac ggaccaattt caacgatctg cgccctgccc
     2821 tgcgcgatac gccaaaaccg gcatatcaac ctgcgcctga atctgaagga ttgcagtaca
     2881 ccacagtagc gaaccaactt accgcgctga agtacaacgc aggttttgaa gcaccggaaa
     2941 ttcagctgcg cgataagacg ctgtatatgt ctggtcaaca atacaaatac cgtgattctc
     3001 gcgaagcggt cgatcgtgcc aaccggattc tggtgaataa cctgccgcaa ggcgttgaga
     3061 agattagcgt gacgcaaaag cgcgagcata tggcgatggt gactaccgaa accgacgtag
     3121 ccagcctgcg caaacagctg gctggtacag cgcctggtca atcagagcaa cttcaacaac
     3181 aacgtgttga agcagaagat ctttctgcct ttggtcgggg ctaccgtatt cgtgaagatc
     3241 gctttagcta ctctttcaac ccaacacttt cacagtcgct gggcggcccg gaagatttct
     3301 atatgttcca gctggggctg atgtccagtg cccgctactg gtttaccgac cacctgctgc
     3361 ttgatggcgg tattttcacc aatatttaca acaactacga caagtttaag tcttcgctgt
     3421 tgcccgcgga ctctaccctg ccccgcgtgc gcacgcatat ccgtgattac gttcgcaatg
     3481 acgtttatct caacaacttg caggcgaact actttgccga cttaggcaat ggtttctatg
     3541 gccaggtgta tggcggttat ctggaaacga tgtacgccgg tgtcggttcc gagctgcttt
     3601 atcgcccgct agatgccagc tgggcgctgg gtgtggacgt taactacgtg aagcaacgtg
     3661 actgggacaa catgatgcgt ttcaccgatt attccacgcc aacaggtttc gtgacggctt
     3721 actggaaccc gccgacgctc aatggcgtac tgatgaaact tagcgttggg caatatctgg
     3781 caaaagataa aggcgcaacg atcgacgtcg ccaaacgctt tgacagcggc gtggcggtag
     3841 gggtatgggc ggcaatcagt aacgtatcta aagatgacta cggcgaaggc ggctttagta
     3901 aaggttttta tatctcgatt ccattcgact tgatgaccat tggacctaac cgcaaccgcg
     3961 cggtggtttc gtggacacca ttgacgcgtg atggtggaca aatgctgtca cgcaaatacc
     4021 agctctatcc aatgacggca gagcgagaag taccggttgg acaataacct gacgtaattc
     4081 ataacattac attgactgat aaatataaac caatgaaaaa gaacattttt aagttttcgg
     4141 tactgacatt ggctgttctg tctttgacag catgtaccct tgttcctggt cagaatttat
     4201 cgaccagtaa caaggatgtc attgaactac ctgacaatca gtatgacctg gacaaaatgg
     4261 tcaatatcta tcccgtcacg ccaggattga ttgatcagtt gcgcgccaaa ccgattatgt
     4321 cgcaggctaa cccggagctg gagcagcaaa tcgcaaacta tgaatatcgt atcggcattg
     4381 gggatgtgtt gatggttacg gtctgggatc acccggaact aaccaccccc gcagggcaat
     4441 accgtagcgc cagtgatacc ggcaactggg ttaacgccga tggtgcgatt ttctacccct
     4501 atattggtcg cctgaaagtg gccgggaaaa cacttaccca ggtacgcaac gagattacag
     4561 cgcgtctgga ttcagtcatt gaaagcccgc aagtcgatgt cagcgtcgcg gcgttccgct
     4621 cgcaaaaagc gtacgtcacc ggcgaagtca gcaaatcggg tcagcagccg attaccaata
     4681 ttcctctgac catcatggat gcgattaacg ccgcgggcgg tctgaccgca gatgcagact
     4741 ggcgcaacgt ggtgctcacc cagaatggcg taaagaccaa agtgaacctc tatgcgctga
     4801 tgcagcgtgg cgacttgcgc cagaacaaac tgctgcaccc gggcgatatt ttatttattc
     4861 cgcgcaacga tgatctgaaa gtcttcgtga tgggtgaagt gggtaaacag agcacgctga
     4921 aaatggaccg cagcggtatg accctggcgg aagcgctggg taacgctgag ggtatgaatc
     4981 aggatgtcgc tgatgcgacg gggatttttg ttattcgtgc aacgcaaaac aagcagaatg
     5041 gcaaaatcgc caatatttat cagctgaatg cgaaggacgc atcggcaatg atactcggaa
     5101 cggaattcca gcttgaacct tacgacattg tttatgtcac taccgcgccg ctggctcgtt
     5161 ggaaccgtgt gatttctctg ctggtaccga cgatttccgg ggtgcatgac ctgacagaga
     5221 cttcacggtg gattcaaaca tggcccaact aaaatttaac tcaatcctgg tggtttgtac
     5281 cggcaacatt tgccgttcgc ccattggcga gcgcttgctg cgtaaacgtc tgccgggcgt
     5341 aaaagtcaaa tcggcaggtg ttcatggtct ggtaaaacac cctgcagatg cgacagcggc
     5401 agatgttgcc gctaatcatg gcgtatctct tgaaggacat gccggacgta agctcactgc
     5461 tgagatggca cgaaattacg atctgattct ggcaatggag tcggaacata ttgctcaggt
     5521 taccgcaatt gctcccgagg tccgcgggaa gacaatgctg tttgggcaat ggctggaaca
     5581 gaaagagatc ccggatccct atcgtaaaag tcaggacgca tttgaacatg tctacggtat
     5641 gttggagcgc gccagtcagg aatgggcgaa gcgcctcagc cggtaatttg agtttataaa
     5701 atacgatgac aactaaaaat atgaatacgc caccaggcag cactcaggaa aatgagatcg
     5761 atctgcttcg tctggtcggc gagttatggg atcaccgtaa gtttattatc agcgtgaccg
     5821 cgttattcac gctgatcgct gtcgcttact cgctgttaag cacaccaatt tatcaggcag
     5881 atactctggt ccaggttgag caaaaacagg gcaacgccat tctcagcggc ctgagcgata
     5941 tgatccctaa ctcatcgccc gagtctgcac cggagatcca actgctgcaa tcgcgcatga
     6001 ttctcggtaa aaccattgct gaactgaatc tgcgcgacat agttgagcag aagtattttc
     6061 cgattgtggg tcgcggctgg gcgagattaa ccaaagaaaa accaggtgag ctggcgatca
     6121 gctggatgca tattccacaa ctgaatggtc aggatcagca actgacactc acggttgggg
     6181 aaaacggcca ctatacactg gaaggtgaag agttcaccgt caatggtatg gtcggccagc
     6241 gtctggaaaa agatggcgtt gcgctgacta tcgcggacat taaggccaaa ccaggaacac
     6301 agtttgtcct gagccagcgt accgaactgg aagcgattaa cgcattgcag gaaaccttta
     6361 ccgttagcga acgcagtaaa gaaagcggga tgctggaact taccatgact ggtgatgatc
     6421 cccagttgat tactcgtatt ctgaacagca tcgctaacaa ctatttgcaa cagaatatcg
     6481 ctcgccaggc ggcgcaggat tcacaaagcc ttgaattctt acagcgccag ttacctgaag
     6541 tgcgcagcga gctggaccag gcggaagaaa aactcaacgt ttatcgccag cagcgcgatt
     6601 cggttgacct taacctggaa gccaaagccg ttcttgagca gattgtgaac gttgataatc
     6661 aactcaatga gctgaccttc cgcgaggcag agatctccca gctgtataag aaagatcacc
     6721 caacttatcg tgcgctgctg gaaaaacgcc agacgctgga gcaagaacgc aaacgcctga
     6781 ataagcgggt atcggcaatg ccttccaccc aacaggaagt gttgcgttta agtcgtgacg
     6841 tagaagcggg ccgtgcggta tatctgcaat tacttaaccg ccagcaggag ttgagtattt
     6901 cgaaatccag tgccattggt aacgtgcgga ttatcgaccc ggcagtcact cagccgcagc
     6961 cagtgaaacc gaaaaaagcg ttgaatgtgg tgcttggttt tattcttggc ctgtttattt
     7021 ctgtgggtgc cgtgctggcg cgtgcgatgt tgcgtcgtgg tgtagaagcc ccggaacaac
     7081 tggaagagca cggcatcagc gtttatgcca ctatcccaat gtccgagtgg ctggataaac
     7141 gcacccgtct gcgtaagaaa aatttatttt ctaatcagca gcgccatcgt actaaaaata
     7201 tccccttcct ggcggtggat aacccggcgg attctgctgt ggaagccgta cgtgcgctac
     7261 gaaccagtct gcatttcgct atgatggaga cggagaataa cattctgatg atcaccggtg
     7321 cgacgccaga cagtggtaaa acgtttgtca gttcaactct ggcagcggtg atcgcccagt
     7381 ccgatcaaaa agtgttattt attgatgccg acttacgccg tggttattcg cataacctgt
     7441 ttaccgtgag taatgaacat ggcttgtcgg aatatctggc aggtaaagat gagctcaaca
     7501 aagtgatcca gcattttggc aaaggaggct ttgatgtgat tactcgcggt caggtgccac
     7561 ctaacccgtc tgaactgctg atgcgcgatc ggatgcgtca attactggaa tgggcgaacg
     7621 accattacga tctggtgatt gtcgatacgc cgccgatgct ggcggtgagt gatgccgcgg
     7681 tcgtggggcg ttctgttggc accagcctgc tggttgcgcg ttttggcttg aacaccgcca
     7741 aagaggtgag tttgtcaatg cagcgtctgg aacaggcagg cgtcaatatt aaaggcgcta
     7801 tcctcaatgg tgtgattaaa cgcgccagca ccgcttacag ttacggctat aactattacg
     7861 gttatagtta ctccgagaaa gagtaaaact gaaagtggcc ggatgcggcg tgaacgcctt
     7921 ttccggtcta cgcattagac agttcttcgt ttgtcatcag cctcagagca ttcaggtaac
     7981 tgaatgctct tttttatgca ttacaaactg cacgccggta tgcgtgcttc attcacgatt
     8041 tgcgtaaaac ctgccaacga acacatgccc tgcgcatttc gctcttcaca tcctgccagg
     8101 gtcagtttca cctctccggg cggcgtattt aatgacagcg gcgttttatc acgcatctgc
     8161 tgtaaagtct ggaagaccag cgaaacctga atccactggc tgttatcgct tagccgacgc
     8221 cagcgttcaa acaccagttc accacctggc ggcgtgttat ccggctgacc gggaagcgtc
     8281 cagttgagct ccagtgcgcc gccgagattt gccagattag tatcgtgtcc ggcgataaac
     8341 agcactgaag tgggtaatgt cacaccatac gcctgttttt gcggtggatg gggcgtcaac
     8401 gctgtcttga tcaaatctaa taacggggtg gcgcggctgc gggcaacctc tggcgtgcgt
     8461 tgtagcaaat aaaattgcgc gttatgcaaa cttagcaagg tgttccactg gtgtgaatcg
     8521 gtgatccttc cccaccccgg ctccggcatt ccctgtgctt gttgcaggag aaatatctcc
     8581 gtcagcattg atgcgaggct taccgcaccg gttaatgaga cattgtcggc gctcaccttg
     8641 agttccgatg gtaatgcctg cgttaatgaa cagctttcgt cctgtttctc acgtttaagg
     8701 cacaagtttg attgcggaaa attaagcacc cgttccagtt cgcgaaacgc cgtttgccga
     8761 tgcccggtaa agtcagcaat tgaccctcct gccctgctga ggatcgcgtc agtcacgttc
     8821 gcattatcca gttggcaaac gccagttttt agaggattaa ataacggatc gggactggac
     8881 gtatctgcct gggtatgtac ggttattgca cagtcaggtg ccagcccggc ggcgaaggct
     8941 tcgcctgttt tacgggtacg ctcgtcgaca tcagcaataa tcgcgacctg accagactgc
     9001 gggcagccct ttttcgccag caatccgtcg gctaccagac gctggcgttg gtaatgtccg
     9061 agataggcga ttagctcacc accgcgcggt gtcagccaac ccagttttac cggccaggtt
     9121 ggccatgcgt ctggggtgac atcctgcatc agttgcgtgg ccttggttgg agcacgcaca
     9181 ccatgacgac tgacaatcac cacactttcc agcttcagct ccggctcact ctgagcgaat
     9241 gcagattgcg gggttaacgg aatcagaaga gataaaaatg ggattaagat cgctttcatc
     9301 gatatgtttc cgcttttcat atctgacatt attgattgcc tgatgcgatg ctaatgcgcc
     9361 gcatcaggcc tacgttcgtt aacttttcag acgcgggtcc agccatacca acacaagggt
     9421 ggagagcgaa cacatcaaca aaatgccgac gaaccaaagt aaataccaca ttgttcctgc
     9481 tccttagtac aactcgtttt cgttacggcg gagagtttct gttgtcatgc gtccccacat
     9541 tttgtagtag ctccagagag tgtagagcaa cacaatgggc aaaaatatca gcacgattac
     9601 caacataatg ctcagcgtca gctgactgga agtgctgtcc cacaacgtca ggctggagat
     9661 tggactcacg cttgacggca tgacaaaggg gaacagcgtg atgcctgccg tgaaaatcac
     9721 gccaaattgc atcaatgacg ccattaaaaa tccccaaccc gggcgaccac gataaatcgc
     9781 catcaccgtc agcaatgggc agaagaaccc cagcagcggg aagatccaca aaacgggcga
     9841 ttcgacgaaa ttattcatcc aggcactagg tagcactgcc accagtttca ttaacggatt
     9901 ggaaggaccg ttagcatcct gggcgagcag tacaaagcca tcaataccga cccacagcca
     9961 gtaacccgcc agcaaaaagc acagcatcac caacagtgca gcgcgtttgg tggccagctg
    10021 tgaacgcaga tgaatcacac caacagtttt cagttgtaac cagatgccac cttgcaaaat
    10081 caccatccca aggctgagca atccgcacaa taaagggaat ggcgtcagca gttgccagaa
    10141 gctgccgaga tactccacgc gtaattgcgg tgtgaaggca aacggcacgc cgagcaacaa
    10201 gttgccgaag gcgataccga agactaccgg cggcaccaga ctgccgatga ccagaccggc
    10261 gtcccacatt ttacgccagc gtgcatcggc gatttttccg cgataatcaa aggccagcgg
    10321 gcggaagaac agtgagcaca gcaccaggat catcgccaca taaaagccgg aaaacgccgc
    10381 tgcatacact ctgggccagg cggcaaataa tgccccacca gcgaggatca accagacctg
    10441 gttgccttcc cagtgtgcac caacgctgtt tatcactatc cggcgttcat catcattacg
    10501 cgccaccagc ggcagcagac agccgatccc catgtcaaat ccgtcggaga tcataaagac
    10561 caccaggatc acgccaatca gcagccacca gatgaagcgc aatgtttcat aatcaaacat
    10621 gattttctcc tttacccctg ttgctgcgtc ggttgttcac tctgcatcgc gctcggcccc
    10681 agacgggcat atttctgcat caggtagact tcggcgatta agaacagggt gtaaagccct
    10741 acgatcatga tcagtgagaa agccagttgt cctgtggtta aagcggagtg cgcggagtat
    10801 gtcggtaaga tgtcctgtat cgcccacggc tgacgaccaa actcggtcat aaaccacccg
    10861 gcttcaatcg caatccacgg caacggcaaa ctccagagcg ccattttcag cacccagcga
    10921 tgctggtcga ttttgccacg cagcgtctgg acaagcgcaa tcagcatcac cagtagcagc
    10981 agggaaccac agcccaccat gatgcggaaa ctccagaata ccggcgcaac ctgaggtatc
    11041 gcgccacgca tcgccgcctg gtactgtgcg gctgtgacat gattcatatc cggcgcatag
    11101 cgggagagca acatgccgta gcccaggtca ccttccagtt cccggaatgc ctgcaacaca
    11161 tgcggctcac gattgccttg cgatatttcc tgcattaaca gccaggccat acgtccgcgt
    11221 tgcaagcgtg ggtaggtttc agccatcaaa ttcttcagac ccggcacggg tttatctaat
    11281 gagtgagtgg cgaggatccc tagcagcgcg ggaattttga tggcaaaggc gttacgctct
    11341 tgatcctgtt ccggccaggc aaccacatgg aacggtgcag gtgcaggttc cgtttgccac
    11401 tccccttcca tcgccgccag ttttaccggt tgtacttgcg cgacttcata cgcagaactg
    11461 tctccgagtt gcagggtacc gataatcgcc agagtaccga agacggaacc gatggcaaac
    11521 gaacgtaatg cgacatcgcg ctcccgtccg cgcagtaaat accaggcgct gatcgccata
    11581 ataaacatgg ccccggtcac gtagcccgcc attacggtgt gcacaaattt cacctggctg
    11641 accgggttaa agaccagctc gctgaagctg gtcatctcca tacgcagggt gtcgatatca
    11701 aaatgcgcac cggtcgggta ttgcatccaa ccgttggcat tcaatatcca caacgcagag
    11761 agatttgaac cgaacgccac cagccacgtc accagcaggt gctggtattt attcagccgt
    11821 tgccagccga agaagaacag cccgacaaag gtggattcga ggaagaaggc cattaatgct
    11881 tccatcgcca gtggtgcgcc aaaaatatcg cccacatagt tggaatagaa tgaccagtta
    11941 gtaccaaact gaaactccat ggtcaggccg gtagccacgc caagagcaaa attgataccg
    12001 aagagcttac cccagaagcg cgtcatatcg cggtagattg ttttgccggt gaccacgtaa
    12061 atggtttcca tgatagccag caaaaaaatc agccccaggg taaggggtac aaataaaaag
    12121 tgatacagcg caggtcagag caaactgcca gcgcgataaa tcaatgacat cccacatgcg
    12181 cactcctgta ggcgaagaga gaaaaataac ccgataatgg tagatctccc tctttatcct
    12241 gaaactctac gaccagaggt attcgccgat tgcgatattc ataagactga aagcgatact
    12301 taccgtcttt ttacgtcggt gcagcttccg ccagccactg acatacctcg ctaagccgct
    12361 gcgcagagtc gaccaaatct tctggcgctg ccagcaccac ttccggtatt gggcagattt
    12421 cataactttc cagtaacggg ccttttaagg tgtccgtaca gcgtaaatgc cagacatggc
    12481 gtaaccccgt ggcgttgata tagctctcgc catagccaat ggtacgaatc tgaatatttc
    12541 ccggcccaca gagacgtgag agaaaaagcc gatcagcctc gctgatgggt aattgcgtca
    12601 gattaatgct gtgcggctgc gcgtcggggt tacgtacatg tgccagtaac tcatgcgcca
    12661 acggtaggcc attcatcagg ccatcaatgg gcggcggtaa cagcgaatct gtcggcaaga
    12721 gattttgcgt tgccgcctgc cacaacgcca gcggcgcgca gccagcctcc agtttgtcct
    12781 ccagcaactt ttcgccgcga cgtctgcgca cccgccataa tccgcagaag atcgcctcct
    12841 gtatttcact ttcactgtcg tcagcctgct gaatgcgcac tgacacttcc ccttccccga
    12901 gaagcgtatt gataaagtga cgatcgtcgg cgttgagatt taacagatcg actgccagca
    12961 cctcgcccaa cggcggttgg cgttcgctta gttgatgttg taactcgttt aacagcgcaa
    13021 tcacctgcgg gctgtgagca cattgctcca gggccgccat actcggttca tcattcacct
    13081 gacaggtgat cggcagtgga ttcatgctga aactgtcatc gttcggttgc gttcctggcc
    13141 ccagcagatg gaaaaatgtt tcgctcatga ggcacgctcc tgctgcggtt cgacaagccc
    13201 gcgcatcagg tttatcagtt ccgcccacgg gtgaatacca ttcagcacgc cgcgatagtt
    13261 tccgccggta aacactaaag tggcaggaaa gcgaaagacg ccaaagcgat cgccgatggc
    13321 ttcgctctgc tcaaggtcag caatcgccac ctgccatgta tagtcgggaa actcgcgcag
    13381 taattcgcca atcattaccg gattatcgct gacctctggc gtgcgtttcg ggtcactgct
    13441 taataacagc acgccgtctg gcgcttgcgt aagccagtcg tcaagacggg attcactgac
    13501 tggcgtccag ccgcgcgcca gcattcgttg ccacaacgca tcaaatggcg tgtcgttgct
    13561 catccctgct cctcctgtgt catccggtac tggcgcaagc gaacgccttc gtaattttcc
    13621 atcgacagac agtcataatt gagacagcgc gattcattag ccggttgcgg cacaattccc
    13681 cacgcagcca gctgcgccag cgccgcctgt tccgcagcgg gcagttgctc ccgtgccagt
    13741 tcgctcaggc taccgccgta gtcgtcgagc attgcgggtt gcagaccgac gagggcaata
    13801 tgtgctggca gatgtccgcg gatatccgcc agcgccaaca cttcggagaa actgttctga
    13861 tgcaggctca ttttcttcgc gctgagataa gccggaatgc gttctccggc ataggttcgc
    13921 agcgttccag gttccagccc gtagtcaatg gcatcgagaa tcaacagatg gctggcgctt
    13981 tcgacatacc ccagcaagtt cagtccctga gtaccgccat cgacaatctc cacatactcg
    14041 ggccagtggt aatgggcata cagccgttcc gccacccgca cgccgaagcc ttcatcggcc
    14101 cacagcaggt tgcccagccc catgaccacc acgcgttgct cgctcatgaa cgctccttgt
    14161 tacttatttt gccaaatttg tggctacggt agccgttgac catggtggag atcaccgtgt
    14221 cgtcggacat gatgtcttca cgcagcgcca tgtagacatg accgatcaca aacgcgccaa
    14281 tcagccacat ccccagccga tgccagctgt gaatgtccat tgagttgcca cccatccagt
    14341 agaaaaattc caccacataa cggaacggcg caaaaatagc gtactggctg tgttcgctat
    14401 acagcgcaaa accagtgatg atcataaaga ccgacatcag gaaatagccg aacatcgccg
    14461 cctgggcgat gggattatgg cctatatcgg cactcggacg ttttgccaga aacagatacc
    14521 agcggatttc ataccacacg ccctgccacc agcttttacg ccataccggc acgataaaca
    14581 gctcgcggga gtatcgattg ccaacaaaag cccagtagat ccgcatcagc aaaaccacgg
    14641 taaaaaccat cccggcgctg aagtgaatta acctgatgta gcccatatag aacagatacg
    14701 tcgcctcgcc gctgacggaa ggtagcggct tgccgataaa gtacccggtg accatcaaca
    14761 ccgccatgca taacaccgtc aaccagtgcc agatgcggac tggcgcttca aagacatagt
    14821 ggctgacaac gttgtcgctt ttctgttgca tgatgattct ccttcgctgt taacgcacct
    14881 gcacagagat cagctcgcta ccgtcgtcgc ccagcacgtg tgttgaacag gcgaggcacg
    14941 ggtcaaagct gtgcagagta cgcaggatct ccagcggttg ctcggggatc gccattttgg
    15001 tgttcatcag cgccgcttca taagcgccaa tctgcccttt gggatcgcgc gggctggcgt
    15061 tccaggtggt cggcaccacg cactggtaga gatcaatctt gccatcgcga atggcggccc
    15121 agtggcctaa cgccccgcgc ggcgcttcgg taaaaccgac accacggcac tctgtcggcc
    15181 aggttgcagg ttcccatttt tccgtggaag cagtggcgag attgccgttt ttcaggttgg
    15241 tcatcagctt gtcgaagaaa tactgcaact tacctgcggc ccactgcgct tcgtgcgcgc
    15301 ggcacaaaat gcggcctaac gttgactgga taccggaaag cggcaggttc aacgccgaca
    15361 tcatgcgatc gaccgactca acggtcgcag catcgccttt gtgataagcg attaacgtgc
    15421 gcgccagcgg ccccacttcc atcgcgttac cgcgccagcg tggcgctttg atccacgagt
    15481 agcgttcctg ttcattcagc tgctgaatgt tggtatcgct gcctttgaca tcgccggggt
    15541 tgtaccacgg gtcggtgatg ccatcgaacg gatgacgccc gacctgatcg ttgggatatc
    15601 gataccaggc gtggtcgaca aactcctgca cctgctgcgg atcaaccaaa tccactggca
    15661 gcacattgtt gaagtcgccg ttaatcaccg cgccgccagg catcagcaga cttttctcgc
    15721 caaagtcgtt ggcaatatcc gggaatgcgc cgtagctgag aacgcattta tcagaaagac
    15781 cagtgccgat ttcgctccac ggtttgttga actgaccgat ggctaaggcg tcggggatca
    15841 tcacgttgtt aatgaagtcc gccgtgcggg tgataattga ctgcaccagg ttcaggcgtt
    15901 ccatattgac tgccccgacc gcgccgcttt cgtcaatgtt gatggcgcaa ggcatcccgc
    15961 cgacaatcca gtttggatgc gggtttttac cgccaaagac cgcgtggatt ttgacaattt
    16021 cacgctggaa atcgagagct tcgagatagt gggcaaagcc catcaggtta gcttctggcg
    16081 gcagtttgta ctgcgggtgc ccccagtagc cattgcggaa gatccccaac tgcccgcctt
    16141 caacaaattt tttcaggcgg ttttgtacgt cgaagaaata gccaggggat gatttcggcc
    16201 aagaggagag actttgcgcc agttcggagg ttttccgcgg gtcggctttc agcgcatcta
    16261 acacatcgat ccagtccatc ccggcaagct gatagaagtg caccagatga tcgtggcacc
    16321 agagcgttgc cagcataatg ttgcggatga tatttgcgtt gtccggcact ttaataccga
    16381 tagcatcttc gatggcgtaa accgaagcca gggcgtgtac gccagtacag acgccgcaga
    16441 tacgttcaac gaacgcccac gcatcgcgcg ggtcgcgccc ttgtaggatg atctccagcc
    16501 cgcgaaacat ggtgccgcag gagacggcat tggtgatcac attctgatcg ttaatattca
    16561 cttcgcagcg catgtggcct tcgatgcgcg taatcgggtc gaccaccagg cggcgtccgg
    16621 cattattgat ggtgtatccc tgagtttcgt actgagtgct catgcctgtt tatcctcatt
    16681 gcctggctga tgttcggttt ctgtaggttg ctggttatga cgtctacgct ggtcaacggc
    16741 gctggcgact gcgtgcacac caacagccgc tgccaccacg ccaagcgcgg ttaaaccgac
    16801 ggtatcggcg gtggaatgag tacccatttg cggaatatcg accacgcggc tgtagaacga
    16861 accgcgatcc cagaaaccat tttccgcaca gcccaggcag ccgtgaccag actggattgg
    16921 gaaagaaacg ccatcattcc agcgtgtgga agaacaggcg ttataggtgg taggcccttt
    16981 gcagcccatt ttgtacaggc agtaaccttt gcgggcagcg tcatcatccc aactctggac
    17041 gaactctccg gcgtcgaagt gggcgcggcg atagcattta tcgtggattc gctgaccata
    17101 gaacatcagc ggacggccca ttctgtcgac atctggcaag cgatcaaagg tcaccatgta
    17161 agtaatgatg gcgctcatca catccgggat cggcgggcag ccaggtactt tgataatggg
    17221 tttgtcggtg atgactttgt cgataggcgt tgcctgcgtc ggattgggcc gcgcggcctg
    17281 cacgcagccc caggacgcgc aggttcccca ggcgataatc gcgctggctc cggcagcggc
    17341 acgtttgagt ttctcaataa acggtcgacc gctgctgata cagaacatcc cctgctcgcc
    17401 cagcggcgga ttaccttcta ctgcgaggat atatttgcca ttgtattgcg tgatgatgtc
    17461 ttcgaagact tcttccgcct gggttccggc ggcagccatc aaagtatcgt cataatcgag
    17521 ggaaatcagg gaaaggatga cgtccttcgc cagtgggtga gcggagcgga taaaagattc
    17581 ggtacagcag gtgcattcca gaccgtggat ccataccacc ggaatgcggg gtttgttctc
    17641 cagcgcccag gcaatctttg gtgccattcc cgcgcctaat cccagcgacg tggcagccag
    17701 actacaatat ttgagaaagc tgcgccgggt aacgccctga cgccgcatgg cctggtaaaa
    17761 tgtttcctcg ttattcatat cgcacgtctc ttctccttgc gacaccggca ggacaccggg
    17821 cgcttatggt cggcaagcga cgtccctgta gcaaggcaaa ccatcgcttc acgtgagatg
    17881 ctgaaaacga aagctcatcc ttctgcactg gcacacgtcg ccagaaagta ttgttaataa
    17941 agcgtagtga aacttttgca caaaacaata caaactgtgt ggatttatct tttagcgata
    18001 aaaatggacc tatttttctt ttggccgggc ggtggggatg tttagccggt tgctaaacga
    18061 gtaaaagaga aggaattcat ggtgatgaaa tgatcaatct gctgttattt cacgctatca
    18121 atgcactttt ggctgttttt caggcaaaca aacaaattag gggtttacac gccgcatcgg
    18181 gatgtttata gtgcgcgtca ttccggaagt gtggccgagc ggttgaaggc accggtcttg
    18241 aaaaccggcg acccgaaagg gttccagagt tcgaatctct gcgcttccgc caaataatca
    18301 aggggttacc aaatggtagc ccctttgctt ttttggctct tggtataaac ttggtatacc
    18361 aattggtata tttttattcg gatgagatta tcggtcatga gcggaacctc aggttcctcg
    18421 tctgatgccg ctcttgcaac acgttacgct gccgagtatt tttgtaaaac atggactgct
    18481 ccaggcctga accaggcaga aggatacaaa gctatctcag atctgtcgca tcactatttt
    18541 cgtgcagaag gctctagccc cccccaatct tttttgtgac accctgtttc actgaacatg
    18601 aagacactca ccttgtccct gatattcatt agcatcatcc tgattattta acttctctat
    18661 t
//
LOCUS       DAZKHF020000071        18581 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2201S31241
            SAMN43267800-rid25106413.denovo.071, whole genome shotgun sequence.
ACCESSION   DAZKHF020000071 DAZKHF020000000
VERSION     DAZKHF020000071.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267800
            Sequence Read Archive: SRR30307263
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 18581)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18581)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 18581)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350581.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350581.2
            Genome Coverage       :: 151x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:21:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,652
            CDSs (total)                      :: 5,557
            Genes (coding)                    :: 5,320
            CDSs (with protein)               :: 5,320
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 237
            CDSs (without protein)            :: 237
            Pseudo Genes (ambiguous residues) :: 0 of 237
            Pseudo Genes (frameshifted)       :: 102 of 237
            Pseudo Genes (incomplete)         :: 130 of 237
            Pseudo Genes (internal stop)      :: 52 of 237
            Pseudo Genes (multiple problems)  :: 41 of 237
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18581
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267800-rid25106413.denovo.071"
                     /serotype="O 26:H11"
                     /isolate="2201S31241"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-01"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            120..812
                     /gene="yieP"
                     /locus_tag="ACHOSC_RS10195"
                     /old_locus_tag="pgaptmp_002039"
     CDS             120..812
                     /gene="yieP"
                     /locus_tag="ACHOSC_RS10195"
                     /old_locus_tag="pgaptmp_002039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312724.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FadR/GntR family transcriptional regulator"
                     /protein_id="HGU4547979.1"
                     /translation="MPLSAQQLAAQKNLSYVLAEKLAQRILKGEYEPGTILPGEIELG
                     EQFGVSRTAVREAVKTLTAKGMVLPRPRIGTRVMPQSNWNFLDQELLTWWMTEENFHQ
                     VIDHFLVMRICLEPQACLLAATVGTAEQKAHLNTLMAEMAALKENFRRERWIEVDMAW
                     HEHIYEMSANPFLTSFASLFHSVYHTYFTSITSDTVIKLDLHQAIVDAIVQSDGDAAF
                     KACQALLRSPDK"
     gene            835..2262
                     /gene="mdtD"
                     /locus_tag="ACHOSC_RS10200"
                     /old_locus_tag="pgaptmp_002040"
     CDS             835..2262
                     /gene="mdtD"
                     /locus_tag="ACHOSC_RS10200"
                     /old_locus_tag="pgaptmp_002040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug transporter subunit MdtD"
                     /protein_id="HGU4547980.1"
                     /translation="MSDKKKRSMAGLPWIAAMAFFMQALDATILNTALPAIAHSLNRS
                     PLAMQSAIISYTLTVAMLIPVSGWLADRFGTRRIFTLAVSLFTLGSLACALSNSLPQL
                     VVFRVIQGIGGAMMMPVARLALLRAYPRNELLPVLNFVAMPGLVGPILGPVLGGVLVT
                     WATWHWIFLINIPIGIAGLLYARKHMPNFTTARRRFDITGFLLFGLSLVLFSSGIELF
                     GEKIVASWIALTVIVTSIGLLLLYILHARHTPNPLISLDLFKTRTFSIGIVGNIATRL
                     GTGCVPFLMPLMLQVGFGYQAFIAGCMMAPTALGSIIAKSMVTQVLRRLGYRHTLVGI
                     TVIIGLMIAQFSLQSPVMAIWMLILPLFILGMAMSTQFTAMNTITLADLTDDNASSGN
                     SVLAVTQQLSISLGVAVSAAVLRVYEGMEGTTTVEQFHYTFITMGIITVASAAMFMLL
                     KTTDGNNLIKRQRKSKPNRVPSESE"
     gene            complement(2228..3220)
                     /gene="rbsR"
                     /locus_tag="ACHOSC_RS10205"
                     /old_locus_tag="pgaptmp_002041"
     CDS             complement(2228..3220)
                     /gene="rbsR"
                     /locus_tag="ACHOSC_RS10205"
                     /old_locus_tag="pgaptmp_002041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose operon transcriptional repressor RbsR"
                     /protein_id="HGU4547981.1"
                     /translation="MATMKDVARLAGVSTSTVSHVINKDRFVSEAITAKVEAAIKELN
                     YAPSALARSLKLNQTHTIGMLITASTNPFYSELVRGVERSCFERGYSLVLCNTEGDEQ
                     RMNRNLETLMQKRVDGLLLLCTETHQPSREIMQRYPTVPTVMMDWAPFDGDSDLIQDN
                     SLLGGDLATQYLIDKGHTRIACITGPLDKTPARLRLEGYRAAMKRAGLNIPDGYEVTG
                     DFEFNGGFDAMRQLLSHPLRPQAVFTGNDAMAVGVYQALYQAELQVPQDIAVIGYDDI
                     ELASFMTPPLTTIHQPKDELGELAIDVLIHRITQPTLQQQRLQLTPILMERGSA"
     gene            complement(3224..4153)
                     /gene="rbsK"
                     /locus_tag="ACHOSC_RS10210"
                     /old_locus_tag="pgaptmp_002042"
     CDS             complement(3224..4153)
                     /gene="rbsK"
                     /locus_tag="ACHOSC_RS10210"
                     /old_locus_tag="pgaptmp_002042"
                     /EC_number="2.7.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004747 - ribokinase activity [Evidence
                     IEA];
                     GO_process: GO:0006014 - D-ribose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribokinase"
                     /protein_id="HGU4547982.1"
                     /translation="MQNAGSLVVLGSINADHILNLQSFPTPGETVTGNHYQVAFGGKG
                     ANQAVAAGRSGANIAFIACTGDDSIGESVRQQLATDNIDITPVSVIKGESTGVALIFV
                     NGEGENVIGIHAGANAALSPALVEAQRERIANASALLMQLESPLESVMAAAKIAHQNK
                     TIVALNPAPARELPDELLALVDIITPNETEAEKLTGIRVENDEDAAKAAQVLHEKGIR
                     TVLITLGSRGVWASVNGEGQRVPGFRVQAVDTIAAGDTFNGALITALLEEKPLPEAIR
                     FAHAAAAIAVTRKGAQPSVPWREEIDAFLDRQR"
     gene            complement(4279..5169)
                     /gene="rbsB"
                     /locus_tag="ACHOSC_RS10215"
                     /old_locus_tag="pgaptmp_002043"
     CDS             complement(4279..5169)
                     /gene="rbsB"
                     /locus_tag="ACHOSC_RS10215"
                     /old_locus_tag="pgaptmp_002043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose ABC transporter substrate-binding protein
                     RbsB"
                     /protein_id="HGU4547983.1"
                     /translation="MNMKKLATLVSAVALSATVSANAMAKDTIALVVSTLNNPFFVSL
                     KDGAQKEADKLGYNLVVLDSQNNPAKELANVQDLTVRGTKILLINPTDSDAVGNAVKM
                     ANQANIPVITLDRQATKGEVVSHIASDNVLGGKIAGDYIAKKAGEGAKVIELQGIAGT
                     SAARERGEGFQQAVAAHKFNVLASQPADFDRTKGLNVMQNLLTAHPDVQAVFAQNDEM
                     ALGALRALQTAGKSDVMVVGFDGTPDGEKAVNDGKLAATIAQLPDQIGAKGVETADKV
                     LKGEKVQAKYPVDLKLVVKQ"
     gene            complement(5194..6159)
                     /gene="rbsC"
                     /locus_tag="ACHOSC_RS10220"
                     /old_locus_tag="pgaptmp_002044"
     CDS             complement(5194..6159)
                     /gene="rbsC"
                     /locus_tag="ACHOSC_RS10220"
                     /old_locus_tag="pgaptmp_002044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002882531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose ABC transporter permease"
                     /protein_id="HGU4547984.1"
                     /translation="MTTQTVSGRRYFTKAWLMEQKSLIALLVLIAIVSTLSPNFFTIN
                     NLFNILQQTSVNAIMAVGMTLVILTSGIDLSVGSLLALTGAVAASIVGIEVNALVAVA
                     AALALGAAIGAVTGVIVAKGRVQAFIATLVMMLLLRGVTMVYTNGSPVNTGFTENADL
                     FGWFGIGRPLGVPTPVWIMGIVFLAAWYMLHHTRLGRYIYALGGNEAATRLSGINVNK
                     IKIIVYSLCGLLASLAGIIEVARLSSAQPTAGTGYELDAIAAVVLGGTSLAGGKGRIV
                     GTLIGALILGFLNNGLNLLGVSSYYQMIVKAVVILLAVLVDNKKQ"
     gene            complement(6164..7669)
                     /gene="rbsA"
                     /locus_tag="ACHOSC_RS10225"
                     /old_locus_tag="pgaptmp_002045"
     CDS             complement(6164..7669)
                     /gene="rbsA"
                     /locus_tag="ACHOSC_RS10225"
                     /old_locus_tag="pgaptmp_002045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose ABC transporter ATP-binding protein RbsA"
                     /protein_id="HGU4547985.1"
                     /translation="MEALLQLKGIDKAFPGVKALSGAALNVYPGRVMALVGENGAGKS
                     TMMKVLTGIYARDAGTLLWLGKETTFTGPKSSQEAGIGIIHQELNLIPQLTIAENIFL
                     GREFVNRFGKIDWKTMYAEADKLLAKLNLRFKSDKLVGDLSIGDQQMVEIAKVLSFES
                     KVIIMDEPTDALTDTETESLFRVIRELKSQGRGIVYISHRMKEIFEICDDVTVFRDGQ
                     FIAEREVASLTEDSLIEMMVGRKLEDQYPHLDKAPGDIRLKVDNLCGPGVNDVSFTLR
                     KGEILGVSGLMGAGRTELMKVLYGALPRTSGYVTLDGHEVVTRSPQDGLANGIVYISE
                     DRKRDGLVLGMSVKENMSLTALRYFSRAGGSLKHADEQQAVSDFIRLFNVKTPSMEQA
                     IGLLSGGNQQKVAIARGLMTRPKVLILDEPTRGVDVGAKKEIYQLINQFKADGLSIIL
                     VSSEMPEVLGMSDRIIVMHEGHLSGEFTREQATQEVLMAAAVGKLNRVNQE"
     gene            complement(7677..8096)
                     /gene="rbsD"
                     /locus_tag="ACHOSC_RS10230"
                     /old_locus_tag="pgaptmp_002046"
     CDS             complement(7677..8096)
                     /gene="rbsD"
                     /locus_tag="ACHOSC_RS10230"
                     /old_locus_tag="pgaptmp_002046"
                     /EC_number="5.4.99.62"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709562.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016872 - intramolecular lyase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-ribose pyranase"
                     /protein_id="HGU4547986.1"
                     /translation="MKKGTVLNSDISSVISRLGHTDTLVVCDAGLPIPKSTTRIDMAL
                     TQGVPSFMQVLGVVTNEMQVEAAIIAEEIKQHNPQLHETLLTHLEQLQKHQGNTIEIR
                     YTTHEQFKQQTAESQAVIRSGECSPYANIILCAGVTF"
     gene            complement(8263..10131)
                     /gene="kup"
                     /locus_tag="ACHOSC_RS10235"
                     /old_locus_tag="pgaptmp_002047"
     CDS             complement(8263..10131)
                     /gene="kup"
                     /locus_tag="ACHOSC_RS10235"
                     /old_locus_tag="pgaptmp_002047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000102304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015079 - potassium ion transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="low affinity potassium transporter Kup"
                     /protein_id="HGU4547987.1"
                     /translation="MSTDNKQSLPAITLAAIGVVYGDIGTSPLYTLRECLSGQFGFGV
                     ERDTVFGFLSLIFWLLIFVVSIKYLTFVMRADNAGEGGILTLMSLAGRNTSARTTSML
                     VIMGLIGGSFFYGEVVITPAISVMSAIEGLEIVAPQLDTWIVPLSIIVLTLLFMIQKH
                     GTAMVGKLFAPIMLTWFLILAGLGLRSIIANPEVLHALNPMWAVHFFLEYKTVSFIAL
                     GAVVLSITGVEALYADMGHFGKFPIRLAWFTVVLPSLTLNYFGQGALLLKNPEAIKNP
                     FFLLAPDWALIPLLIIAALATVIASQAVISGVFSLTRQAVRLGYLSPMRIIHTSEMES
                     GQIYIPFVNWMLYVAVVIVIVSFEHSSNLAAAYGIAVTGTMVLTSILSTTVARQNWHW
                     NKYFVALILIAFLCVDIPLFTANLDKLLSGGWLPLSLGTVMFIVMTTWKSERFRLLRR
                     MHEHGNSLEAMIASLEKSPPVRVPGTAVYMSRAINVIPFALMHNLKHNKVLHERVILL
                     TLRTEDAPYVHNVRRVQIEQLSPTFWRVVASYGWRETPNVEEVFHRCGLEGLSCRMME
                     TSFFMSHESLILGKRPWYLRLRGKLYLLLQRNALRAPDQFEIPPNRVIELGTQVEI"
     gene            10354..11850
                     /gene="ravA"
                     /locus_tag="ACHOSC_RS10240"
                     /old_locus_tag="pgaptmp_002048"
     CDS             10354..11850
                     /gene="ravA"
                     /locus_tag="ACHOSC_RS10240"
                     /old_locus_tag="pgaptmp_002048"
                     /EC_number="3.6.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418202.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase RavA"
                     /protein_id="HGU4547988.1"
                     /translation="MAHPHLLAERISRLSSSLEKGLYERSHAIRLCLLAALSGESVFL
                     LGPPGIAKSLIARRLKFAFQNARAFEYLMTRFSTPEEVFGPLSIQALKDEGRYERLTS
                     GYLPEAEIVFLDEIWKAGPAILNTLLTAINERQFRNGAHVEKIPMRLLVAASNELPEA
                     DSSLEALYDRMLIRLWLDKVQDKANFRSMLTSQQDENDNPVPDALQVTDEEYERWQKE
                     IGEITLPDHVFELIFMLRQQLDKLPDAPYVSDRRWKKAIRLLQASAFFSGRSAVAPVD
                     LILLKDCLWYDAQSLNLIQQQIDVLMTGHAWQQQGMLTRLGAIVQRHLQLQQQQSDKT
                     ALTVIRLGGIFSRRQQYQLPVNVTASTLTLLLQKPLKLHDMEVVHISFERSALEQWLS
                     KGGEIRGKLNGIGFAQKLNLEVDSAQHLVVRDVSLQGSTLALPGSSAEGLPGEIKQQL
                     EELESDWRKQHALFSEQQKCLFIPGDWLGRIEASLQDVGAQIRQAQQC"
     gene            11844..13295
                     /gene="viaA"
                     /locus_tag="ACHOSC_RS10245"
                     /old_locus_tag="pgaptmp_002049"
     CDS             11844..13295
                     /gene="viaA"
                     /locus_tag="ACHOSC_RS10245"
                     /old_locus_tag="pgaptmp_002049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase RavA stimulator ViaA"
                     /protein_id="HGU4547989.1"
                     /translation="MLTLDTLNVMLAVSEEGLIEEMIIALLASPQLAVFFEKFPRLKA
                     AITDDVPRWREALRSRLKDARVPPELTEEVMCYQQSQLLSTPQFIVQLPQILDLLHRL
                     NSPWAEQARQLVDANSTITSALHTLFLQRWRLSLIVQATTLNQQLLEEEREQLLSEVQ
                     ERMTLSGQLEPILADNNTAAGRLWDMSAGQLKRGDYQLIVKYGEFLNEQPELKRLAEQ
                     LGRSREAKSIPRNDAQMETFRTMVREPATVPEQVDGLQQSDDILRLLPPELATLGITE
                     LEYEFYRRLVEKQLLTYRLHGESWREKVIERPVVHKDYDEQPRGPFIVCVDTSGSMGG
                     FNEQCAKAFCLALMRIALAENRRCYIMLFSTEIVRYELSGPQGIEQAIRFLSQQFRGG
                     TDLASCFRAIMERLQSREWFDADAVVISDFIAQRLPDDVTSKVKELQRVHQHRFHAVA
                     MSAHGKPGIMRIFDHIWRFDTGMRSRLLRRWRR"
     gene            complement(13300..14292)
                     /gene="asnA"
                     /locus_tag="ACHOSC_RS10250"
                     /old_locus_tag="pgaptmp_002050"
     CDS             complement(13300..14292)
                     /gene="asnA"
                     /locus_tag="ACHOSC_RS10250"
                     /old_locus_tag="pgaptmp_002050"
                     /EC_number="6.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004071 - aspartate-ammonia ligase
                     activity [Evidence IEA];
                     GO_process: GO:0006529 - asparagine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate--ammonia ligase"
                     /protein_id="HGU4547990.1"
                     /translation="MKTAYIAKQRQISFVKSHFSRQLEERLGLIEVQAPILSRVGDGT
                     QDNLSGCEKAVQVKVKALPDAQFEVVHSLAKWKRQTLGQHDFSAGEGLYTHMKALRPD
                     EERLSPLHSVYVDQWDWERVMGDGERQFSTLKSTVEAIWAGIKATEAAVSEEFGLAPF
                     LPDQIHFVHSQELLSRYPDLDAKGRERAIAKDLGAVFLVGIGGKLSDGHRHDVRAPDY
                     DDWSTPSELGHAGLNGDILVWNPVLEDAFELSSMGIRVDADTLKHQLALTGDEDRLQL
                     EWHQALLRGEMPQTIGGGIGQSRLTMLLLQLPHIGQVQCGVWPAAVRESVPSLL"
     gene            14444..14902
                     /gene="asnC"
                     /locus_tag="ACHOSC_RS10255"
                     /old_locus_tag="pgaptmp_002051"
     CDS             14444..14902
                     /gene="asnC"
                     /locus_tag="ACHOSC_RS10255"
                     /old_locus_tag="pgaptmp_002051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator AsnC"
                     /protein_id="HGU4547991.1"
                     /translation="MENYLIDNLDRGILEALMGNARTAYAELAKQFGVSPGTIHVRVE
                     KMKQAGIITGARIDVSPKQLGYDVGCFIGIILKSAKDYPSALAKLESLDEVTEAYYTT
                     GHYSIFIKVMCRSIDALQHVLINKIQTIDEIQSTETLIVLQNPIMRTIKP"
     gene            14992..15435
                     /gene="mioC"
                     /locus_tag="ACHOSC_RS10260"
                     /old_locus_tag="pgaptmp_002052"
     CDS             14992..15435
                     /gene="mioC"
                     /locus_tag="ACHOSC_RS10260"
                     /old_locus_tag="pgaptmp_002052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FMN-binding protein MioC"
                     /protein_id="HGU4547992.1"
                     /translation="MADITLISGSTLGGAEYVAEHLAEKLEEAGFTTETLHGPLLEDL
                     SASGIWLIISSTHGAGDIPDNLSPFYEALQEQKPDLSAVRFGAIGIGSREYDTFCGAI
                     DKLEAELKNSGAKQTGETLKINILDHDIPEDPAEEWLGSWINLLK"
     gene            15813..17702
                     /gene="mnmG"
                     /locus_tag="ACHOSC_RS10265"
                     /old_locus_tag="pgaptmp_002053"
     CDS             15813..17702
                     /gene="mnmG"
                     /locus_tag="ACHOSC_RS10265"
                     /old_locus_tag="pgaptmp_002053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000499793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0002098 - tRNA wobble uridine modification
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis enzyme MnmG"
                     /protein_id="HGU4547993.1"
                     /translation="MFYPDPFDVIIIGGGHAGTEAAMAAARMGQQTLLLTHNIDTLGQ
                     MSCNPAIGGIGKGHLVKEVDALGGLMAKAIDQAGIQFRILNASKGPAVRATRAQADRV
                     LYRQAVRTALENQPNLMIFQQAVEDLIVENDRVVGAVTQMGLKFRAKAVVLTVGTFLD
                     GKIHIGLDNYSGGRAGDPPSIPLSRRLRELPLRVGRLKTGTPPRIDARTIDFSVLAQQ
                     HGDNPMPVFSFMGNASQHPQQVPCYITHTNEKTHDVIRSNLDRSPMYAGVIEGVGPRY
                     CPSIEDKVMRFADRNQHQIFLEPEGLTSNEIYPNGISTSLPFDVQMQIVRSMQGMENA
                     KIVRPGYAIEYDFFDPRDLKPTLESKFIQGLFFAGQINGTTGYEEAAAQGLLAGLNAA
                     RLSADKEGWAPARSQAYLGVLVDDLCTLGTKEPYRMFTSRAEYRLMLREDNADLRLTE
                     IGRELGLVDDERWARFNEKLENIERERQRLKSTWVTPSAEAAAEVNAHLTAPLSREAS
                     GEDLLRRPEMTYEKLTTLTPFAPALTDEQAAEQVEIQVKYEGYIARQQDEIEKQLRNE
                     NTLLPATLDYRQVSGLSNEVIAKLNDHKPASIGQASRISGVTPAAISILLVWLKKQGM
                     LRRSA"
     gene            17766..18389
                     /gene="rsmG"
                     /locus_tag="ACHOSC_RS10270"
                     /old_locus_tag="pgaptmp_002054"
     CDS             17766..18389
                     /gene="rsmG"
                     /locus_tag="ACHOSC_RS10270"
                     /old_locus_tag="pgaptmp_002054"
                     /EC_number="2.1.1.170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA];
                     GO_process: GO:0046118 - 7-methylguanosine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(527)-N(7))-methyltransferase
                     RsmG"
                     /protein_id="HGU4547994.1"
                     /translation="MLNKLSLLLKDAGISLTDHQKNQLIAYVNMLHKWNKAYNLTSVR
                     DPNEMLVRHILDSIVVAPYLQGERFIDVGTGPGLPGIPLSIVRPEAHFTLLDSLGKRV
                     RFLRQVQHELKLENIEPVQSRVEEFPSEPPFDGVISRAFASLNDMVSWCHHLPGEQGR
                     FYALKGQMPEDEIALLPEEYQVESVVKLQVPALDGERHLVVIKANKI"
BASE COUNT         4674 a         4831 c         4587 g         4489 t
ORIGIN      
        1 tttttcagca aaagccccgt taatgtgcca taaaacaagc aatttgccgt ttttgcaacc
       61 gtaatcacac ttcctggtgg catactaagg aggaataaaa agaaaaagga agattagtaa
      121 tgcctttaag cgcacaacag ttagctgcac agaaaaacct ttcgtatgtt ctggctgaga
      181 agctggcgca acgcatctta aaaggtgaat atgaacccgg caccattttg cctggtgaga
      241 ttgagctggg cgagcaattt ggagtgagtc gtacagcggt acgcgaagcg gtcaaaacgt
      301 taacggcaaa agggatggtt ttaccgcgac cgcgaattgg tacccgggtc atgccacaat
      361 caaactggaa ttttcttgat caggaattgc ttacctggtg gatgacagaa gagaactttc
      421 atcaggtcat cgatcacttt ctggttatgc gtatctgtct ggagccgcaa gcttgcctac
      481 tggctgcaac ggttggcacc gcggaacaga aggcgcatct caatacgtta atggccgaaa
      541 tggcggcatt aaaagagaac tttcgccgtg aacgctggat cgaagtcgat atggcctggc
      601 atgagcatat ctatgaaatg agcgccaatc cgtttttgac ctcattcgcc tcgctattcc
      661 attcggttta tcacacttac ttcacgtcaa ttaccagcga cacagtgata aagctggatc
      721 tgcaccaggc gattgtcgat gcgattgtcc aaagcgatgg cgacgcggca tttaaagctt
      781 gtcaggcgct gctacgctca cctgataagt gacaacccga taataggaca ttgaatgagc
      841 gataaaaaga agcgcagtat ggcgggtttg ccgtggatcg cggcgatggc cttcttcatg
      901 caggcacttg atgccactat tctgaatacc gccttacccg caatcgctca tagccttaat
      961 cgttctcctc tcgcgatgca atcagccatc atcagttata cgctgacggt ggcgatgctt
     1021 attccggtaa gcggatggct agccgatcgc ttcggtacgc gtcgcatttt tacccttgcc
     1081 gtgagtctgt tcacattggg ttctctggcc tgcgcacttt ctaattcgct accacagctg
     1141 gttgtcttcc gggttattca ggggataggc ggcgcaatga tgatgcctgt tgctcggctg
     1201 gccttactgc gcgcttatcc tcgtaatgaa cttcttccag tattgaattt tgtcgccatg
     1261 ccgggtctgg tggggccaat tttaggcccc gttcttggcg gcgtgctggt cacctgggca
     1321 acctggcact ggatattttt aatcaatatc cccataggta ttgcgggcct tctttacgcg
     1381 cgcaaacata tgcccaattt caccaccgca cgacgcagat tcgatatcac tggctttttg
     1441 ctgtttggcc tcagccttgt tctcttctca agcggaatag agctattcgg ggaaaagatt
     1501 gtcgccagct ggattgcctt gacggtaatt gtcaccagca tcgggttact gcttctctat
     1561 attctccatg cgcgacacac gccaaaccca ttaatttcat tagatttatt taaaacccgc
     1621 actttctcga tcggtatcgt aggcaatatt gcaacccgtc tggggaccgg ttgtgtaccg
     1681 ttccttatgc cattgatgtt acaggtagga tttggttatc aggcgtttat tgccggctgt
     1741 atgatggcac cgacagcgtt aggttccatt attgcaaaat cgatggttac ccaagtctta
     1801 cgtcgtctgg gctatcgcca tacgttagtg gggatcacgg tgattattgg gctaatgatc
     1861 gctcagttct ctttgcaatc accggtaatg gcgatatgga tgctgatctt gccgttgttt
     1921 atattaggga tggctatgtc gacgcaattt accgcgatga ataccatcac acttgccgat
     1981 ctgaccgatg acaacgccag cagcggtaac agtgttctgg cggtcacgca gcaactgtcg
     2041 attagtttag gcgttgctgt aagtgcggcc gtccttcgcg tttatgaagg gatggaaggc
     2101 acaacgactg tcgaacaatt ccactatacg tttatcacga tgggcattat tactgttgct
     2161 tcagcagcaa tgttcatgct tctgaaaaca accgatggta ataatttgat caaaagacag
     2221 cgtaaatcta agccgaaccg cgttccatca gaatcggagt aagttgtaat cgttgttgct
     2281 gaagggtcgg ctgggttatc cgatggatga gtacatcaat cgccagctcc cccagttcat
     2341 ctttcggttg gtggatagtg gttaatggtg gcgtcataaa gcttgccagt tcgatatcgt
     2401 catagccaat caccgcgata tcctgcggaa cctgtaactc tgcctgatat aacgcctggt
     2461 aaacgccaac agccatagcg tcatttccgg taaagacggc ctgaggacgc agcggatgtg
     2521 ataacagttg gcgcatggcg tcaaacccgc cgttaaattc aaaatcacca gtgacttcat
     2581 agccatcagg aatgttgaga cccgcacgtt tcatcgccgc ccgataacct tccaaccgca
     2641 ggcgcgccgg agttttatcc agcggaccgg taatacaggc gatacgggta tgacctttat
     2701 cgatcagata ttgcgttgct aagtctccgc ccagcaacga gttatcctga ataagatcgc
     2761 tgtcgccatc gaacggagcc cagtccatca tcacagtagg cactgtcgga taacgttgca
     2821 tgatttcacg cgaaggttga tgcgtttcgg tgcacagtaa cagcaagcca tcaacgcgtt
     2881 tttgcatcag cgtttccaga ttgcgattca tccgctgttc atctccttcg gtattgcaaa
     2941 ggacgagact ataaccgcgt tcgaagcagc tgcgttcaac gccacgcacc agttctgaat
     3001 agaaaggatt ggtactggca gtgatcaaca tgccaatggt atgtgtttga ttgagtttga
     3061 ggctacgcgc cagggctgat ggcgcgtaat tgagttcttt aatcgccgct tcaactttgg
     3121 cggtaatcgc ttcactgacg aagcgatctt tattgataac gtgagaaact gttgaggtag
     3181 aaacgcccgc caggcgggca acatctttca ttgtagccaa gcgtcacctc tgcctgtcta
     3241 aaaatgcgtc gatctcttca cgccacggta cggaaggttg tgcgccttta cgtgttacgg
     3301 caatcgcagc ggcagcatgg gcaaaccgaa tcgcctctgg caatggtttt tcttccagca
     3361 acgccgtgat taacgcgccg ttaaaggtat ctccggcagc aatggtatcg acagcctgca
     3421 cccggaatcc aggaacgcgc tgaccttcac cattcacgct agcccataca ccacgacttc
     3481 ctaaagtaat cagtacagta cggatacctt tttcatgcag tacctgcgcc gccttcgctg
     3541 catcttcatc attttcaaca cgaataccgg tgagcttttc tgcttccgtt tcgtttggcg
     3601 taataatgtc caccagcgcc agcagttcgt caggaagttc gcgagccgga gccgggttaa
     3661 gcgcaacgat agtcttattt tgatgggcga ttttcgccgc tgccatcaca ctttcgagtg
     3721 gtgattccag ctgcattaat aatgctgacg cgttggcaat acgctcacgt tgcgcttcca
     3781 ccagcgccgg ggaaagggca gcattagcgc cggcatgaat accgatgaca ttctcacctt
     3841 cgccattaac aaaaatcagc gccacacctg ttgattcgcc tttgatcacg ctgaccggag
     3901 taatatcaat gttatcagtg gcgagctgct ggcgaacgct ctcaccaatg ctgtcatcac
     3961 ccgtacaggc aataaacgcg atattcgcac cgctacgccc agcagccaca gcctgattcg
     4021 cgcctttgcc gccaaatgca acctgatagt ggttaccggt tacggtttcg cctggagtag
     4081 gaaaagattg aagattaaga atgtggtcag cattaatgct gccaagaaca acgaggctgc
     4141 ctgcgttttg catattcggg atgtccataa aatgcgccac cgtgttaggg tggcgcgtgc
     4201 cctgcttttc tttatattac acgttcatct gtcgatgacg tatttatgtc accatcaggt
     4261 catacaacct gattaaaact actgcttaac aaccagtttc agatcaaccg gatacttagc
     4321 ctgaactttc tcgcctttca gcactttatc tgcggtttcg acgcctttcg cgccaatctg
     4381 atcgggtagc tgagcgatag tcgctgctag tttgccatca ttcaccgctt tttcgccatc
     4441 cggtgtaccg tcaaatccga cgaccatcac atccgattta ccggcagttt gcagtgcgcg
     4501 cagcgcgccc agcgccattt catcattctg cgcgaataca gcctgaacat ccggatgagc
     4561 ggtcaacagg ttctgcatta cgttcaaacc tttagtgcga tcaaaatctg ctggctggct
     4621 ggcaagaaca ttaaacttgt gagcagcaac ggcctgctgg aagccttcgc cacgttcacg
     4681 ggctgcggat gtaccagcaa tgccttgcag ctcgataact ttggcacctt cacccgcttt
     4741 cttcgcgatg taatcaccgg cgattttgcc acccagtacg ttatcagaag caatgtggct
     4801 caccacttca cctttcgttg cctggcggtc aagagtgata accgggatgt tcgcctggtt
     4861 agccatcttc acagcattac ctactgcgtc ggagtcggtc gggttaatca gcagaatttt
     4921 ggtgccgcga acggttaagt cctgcacgtt cgccagctct ttcgccgggt tgttctggga
     4981 gtccagcacc accaggttat agccaagctt atccgcctct ttctgcgcgc catctttcag
     5041 cgatacaaag aacgggttgt taagcgtgga gaccaccagc gcgatggtgt cttttgccat
     5101 cgcattcgca ctgacggtgg cgcttagcgc aacagcggaa accagggtag ccagtttttt
     5161 catgttcata ttcaagatgt cctgtagtcg ttattactgc tttttgttgt ctaccagcac
     5221 cgccagcaaa atcaccaccg ctttgacgat catctggtaa taggaggaaa cacctaacaa
     5281 attcaatcca ttattaagga agccaagaat taatgcgccg atcaacgtcc caacaatgcg
     5341 accttttccg cccgccagac tcgtaccgcc cagaaccacc gcagcaatag catccagctc
     5401 atagccagtc cccgccgtgg gttgtgcgga ggagagacgc gccacttcaa tgatcccggc
     5461 cagcgatgcc agcagaccac aaagagaata gacgatgatt ttgattttat tgacgttgat
     5521 accagaaaga cgcgttgccg cttcgttgcc gcccagcgcg tagatgtaac gccccagacg
     5581 cgtgtgatgc agcatgtacc aggccgcgag gaagacaatc cccatgatcc agactggcgt
     5641 cggtacgccc agcggacgac caataccaaa ccagccaaac agatcggcgt tctcagtaaa
     5701 gccggtattc actgggctac cgttggtata aaccatggtc acgccgcgca gtaaaagcat
     5761 cataaccagc gtagcgataa acgcctgaac gcgacctttc gctacaatca ccccggttac
     5821 cgcaccaatt gcggcaccta acgcgagagc ggcagcgaca gccaccagcg cattgacttc
     5881 aatgccgacg atagatgcag caactgcgcc ggttagcgcc aacagagaac ctacagacaa
     5941 gtcgatgccc gacgtcagga tcaccagcgt catcccgacc gccataatgg cgttcaccga
     6001 ggtttgctgg agaatattga ataagttatt gatggtgaaa aagttcgggc ttaacgtcga
     6061 gacaatcgcg atcagcacca gcagagcgat aagcgatttc tgctccatca gccacgcttt
     6121 cgtgaaataa cggcgaccag agacagtctg ggttgtcatt tttttactcc tgattcacgc
     6181 gattaagctt gcccacagcg gcagccatta acacttcctg ggtggcctgc tcacgagtaa
     6241 attccccgct gagatgccct tcatgcatga cgatgatgcg atcgctcatg cctaatactt
     6301 ctggcatctc cgatgacacc agaatgatgc tcaagccatc ggctttgaac tggttaatca
     6361 gttgatagat ctcttttttc gcgccgacat ctacgccacg ggtaggttca tcaaggatca
     6421 acactttggg gcgtgtcatc agaccgcggg caatcgccac tttttgctga ttgccaccgg
     6481 aaagcagacc aattgcctgt tccatcgacg gtgttttcac attaaacaga cgaatgaaat
     6541 cactcacagc ctgctgttca tcggcatgct tcaaactgcc gccagcgcgg ctgaagtagc
     6601 gcagcgctgt cagcgacatg ttctctttta ctgacatgcc caacactaaa ccgtcacgtt
     6661 tacggtcttc ggagatatac acaatgccgt ttgccaggcc atcctgcggt gaacgggtaa
     6721 cgacttcatg cccatccagg gtgacgtaac cgctggtgcg cggtagtgcg ccgtagagca
     6781 ctttcatcag ttcggtacga cctgcgccca tcaaaccaga gacgccaaga atttcgcctt
     6841 tgcgtaaagt aaaagagaca tcgttaacgc caggtccgca gagattatcg actttcaggc
     6901 ggatatctcc cggcgctttg tccaggtgtg gatattgatc ttccagcttg cggcccacca
     6961 tcatctcaat cagcgaatct tcggtcagtg atgccacttc gcgctcagca ataaattgcc
     7021 cgtcacgaaa aacggtaacg tcatcgcaaa tctcgaagat ttctttcatg cggtgggaga
     7081 tatagacaat accgcggcct tgcgatttta gttcgcggat gacgcggaac agggattcgg
     7141 tttcggtatc ggtcagcgca tcggtcggtt catccataat gatgactttc gactcaaagc
     7201 tcagcacttt ggcgatttca accatttgct ggtcaccgat ggaaagatcg cccaccagct
     7261 tgtcgctttt aaagcgcagg ttaagtttag ccagcaattt atccgcttcg gcatacatgg
     7321 ttttccagtc aattttgcca aagcgattaa caaactcacg accgaggaaa atgttttcgg
     7381 caatggtcaa ctgcgggatc aggttcagtt cctgatggat aatcccaatc ccggcttcct
     7441 gggaagattt cggcccggta aatgtcgttt ctttccccag ccataaaagc gtgccggcat
     7501 cgcgagcata gataccagta agcactttca tcatggtgga tttacccgcg ccgttttcgc
     7561 ccaccagcgc catcacgcgg cccggataga catttaacgc tgcgcccgag agggctttta
     7621 cgcccgggaa ggctttatcg atgcctttaa gctgaagtaa tgcttccatg acggcctcag
     7681 aacgtcacgc cagcacagag aatgatattc gcatacggag aacattctcc gctgcgaatt
     7741 accgcctgac tttctgcggt ttgttgtttg aattgttcat gcgtggtgta acgaatttca
     7801 atggtatttc cctggtgttt ttgcagctgc tcaaggtgag tgagcaacgt ttcgtggagt
     7861 tgcggattat gttgtttgat ctcttccgcg ataatggcag cctcgacctg catttcattt
     7921 gtgacgacgc ccagcacctg cataaaagaa ggtacaccct gggttaatgc catatcgata
     7981 cgcgttgtac ttttggggat aggtaaacca gcatcacaca ccaccagcgt atcggtatgt
     8041 cccagacggg agatcaccga tgaaatatca gaattaagaa cggtgccttt tttcattttt
     8101 ttctccatca gcgaaacgtt tcgctgaccc gcagtttaat cctgactgag ttgggaaaac
     8161 catcgtgacg ttacggaaat gtgatcaacc tcgaaacgtt ttacatggtg attaaccatg
     8221 aaaacaaaaa cgccccctta tggagaaagg aggcgtctgg cgttagattt cgacctgagt
     8281 acccagttcg ataaccctgt ttggcggaat ttcaaattga tctggtgcac gcagcgcatt
     8341 acgttgcagc agcaagtaca gcttgccgcg cagacgcaaa taccacgggc gtttgccgag
     8401 gatcaacgac tcatgcgaca taaagaagga ggtttccatc atccggcaac ttaatccttc
     8461 cagaccgcag cggtggaaaa cttcttccac gtttggcgtt tctcgccaac cataacttgc
     8521 caccacgcgc cagaaagtgg gcgacagttg ttcaatctgt acccgacgga cattatggac
     8581 atatggcgcg tcttcggtac gcagagttaa cagaatcacc cgctcatgca ataccttgtt
     8641 atgtttaagg ttatgcatca gcgcaaaggg aatgacgttg attgcacgcg acatatacac
     8701 cgcggtcccg ggcacgcgaa caggcggtga tttctccagc gaagcaatca tcgcttccag
     8761 agagttacca tgttcatgca tccgccgcag caagcggaaa cgctcgctct tccaggtggt
     8821 catcacgata aacatcacag taccgaggct caatggcaac cagccgccgg agagcagttt
     8881 atcgaggtta gcggtgaaca atggaatatc gacacaaagg aaagcaatca ggatcagcgc
     8941 aacaaaatac ttattccagt gccagttctg acgtgccacg gtagtcgaga gaatagacgt
     9001 cagcaccatg gttccggtca ccgcaatccc gtacgccgcc gccaggttgc tggagtgctc
     9061 aaagctgaca atcacaatca cgaccgcgac atagagcatc cagttcacaa acggaatata
     9121 gatttgccct gactccattt cggaggtgtg aataatgcgc atcggcgaca aatatcccag
     9181 acgtaccgcc tgacgcgtca atgagaagac gccagagata accgcctgcg aggcaattac
     9241 cgtcgccagt gcggcgatga tcagcagcgg gatcagcgcc cagtccggtg ccaacaggaa
     9301 gaacgggttc ttaatcgctt ccgggttctt taacaacagc gctccctgac cgaagtaatt
     9361 aagggtcaag gaaggtaata cgacggtgaa ccacgccagg cgaataggga acttaccaaa
     9421 gtgccccata tcagcataca gcgcctcgac ccccgtaatc gacaacacca ctgcccctaa
     9481 tgcaataaaa gaaaccgttt tgtattcaag gaagaaatgc accgcccaca ttggattcag
     9541 cgcatgcagc acttccgggt tagcaataat gctacgtaac cccagtcctg ccagaatcaa
     9601 aaaccaggtc agcatgatcg gcgcaaacag cttaccgacc atagccgtgc catgtttttg
     9661 aatcataaat aataaagtga gaacgataat tgagagggga actatccagg tatccagctg
     9721 cggggcgacg atttccagac cttcaatggc tgacatcacc gaaatagcgg gtgttatgac
     9781 gacttcacca tagaaaaagc tgccgccgat tagccccata atcaccagca ttgatgtggt
     9841 tcgcgccgac gtattacgcc cggcaagcga catcaacgtc aggatccccc cctcaccggc
     9901 gttatctgcc cgcatcacga aggtgagata tttaatggaa accacaaaga ttagcagcca
     9961 gaagatcagc gataaaaagc caaacacggt atcgcgttca acgccaaaac caaactggcc
    10021 ggacaaacat tcacgaagtg tatataacgg gctggtacca atatcgccgt agacaactcc
    10081 aatcgccgcg agggtaatcg cgggcaatga ttgcttatta tcagtgctca tagactagtc
    10141 tttcgttgaa atatgaaatg tgtgcttagt cccttggccc acaaaaaagc gcacagtatg
    10201 cacgattaac ggcaaaatcg tactcctaaa tgcggccaca ttaacctggc tcaaagaaaa
    10261 ataccccccc tttgagacta ttttagccac cagcaagaaa cgtctatact cgcaatttac
    10321 gcagaacttt tgacgaaagg acgccacttc attatggctc accctcattt attagcggaa
    10381 agaatttccc gcctgagcag ttcgttggaa aaggggcttt atgaacgtag ccacgccatc
    10441 cgcttgtgtt tattagcggc attaagtggt gaaagtgtgt tcctccttgg cccgccaggt
    10501 attgccaaaa gtttgatcgc ccggcgctta aaattcgcct ttcagaatgc ccgcgcgttt
    10561 gaatatctga tgacccgctt ctccacgccg gaagaagttt ttggtcccct ttctattcag
    10621 gcgctaaaag atgaagggcg ctatgaacgt ttaaccagcg gttacctgcc ggaagcagaa
    10681 attgtctttc tggatgagat ctggaaagcg ggcccggcaa ttcttaatac cttgctcacc
    10741 gccattaacg agcgccagtt ccgcaacggt gcacacgtag aaaaaatccc gatgcgtctg
    10801 ctggtggcgg cctccaacga gctgccggaa gcagacagca gtctggaagc gttatatgac
    10861 cgcatgctga ttcgtctgtg gttagataaa gtgcaggata aagcgaattt ccgctccatg
    10921 ctgaccagtc aacaggatga aaacgacaat ccggttcctg atgccttgca ggtcacagat
    10981 gaagaatatg aacgctggca gaaagagatt ggtgaaatta cgctgcccga tcatgtattt
    11041 gagctgattt ttatgctgcg ccagcaactg gataaattac cggatgcgcc ttatgtctcg
    11101 gatcgtcgct ggaaaaaagc gatccgatta ttgcaggcca gcgccttttt tagcggtcgc
    11161 agtgctgttg ccccggtaga tctcattttg ctgaaagatt gcctgtggta tgacgcgcaa
    11221 agcctgaatt tgatacaaca acaaattgat gtattgatga ccggtcacgc ctggcaacag
    11281 caagggatgt tgactcgcct tggcgcgatt gtgcaacgtc acctgcaact ccagcagcaa
    11341 caaagcgata aaacagcctt aacggtaatt cgtctgggcg gcattttcag ccgtcgtcag
    11401 cagtatcaac ttcctgttaa cgttactgct tccactctga ctctactgct gcaaaaaccg
    11461 ttaaaactgc atgatatgga agtggttcat atctcctttg agcgtagcgc gctggaacag
    11521 tggctgagca aaggtggtga aattcgcggt aaactgaacg gtatcggctt tgcccagaaa
    11581 ctgaatctgg aagttgatag cgcccaacat cttgtcgttc gcgatgtaag tttacaaggc
    11641 agtacgctgg cacttcccgg ttcatcggct gaaggtctgc ctggtgaaat aaaacaacaa
    11701 ctggaagagc ttgaaagcga ctggcgcaag caacacgctt tattcagcga acagcaaaaa
    11761 tgtctgttta tccctggcga ctggttaggt cgcattgaag ccagcctaca ggatgtcggt
    11821 gcacagattc gccaggcaca acaatgctaa cgctggatac gcttaatgtg atgctggccg
    11881 tcagcgaaga gggattgatc gaagagatga tcatcgcact gctggcctca ccgcagctgg
    11941 cggtcttctt tgaaaaattc ccacgcctga aggcggcaat cactgatgat gttccccgct
    12001 ggcgtgaagc gctgcgcagt cggctgaaag atgcccgagt cccgccggaa ctcaccgaag
    12061 aggtgatgtg ctatcagcaa agccagctcc tctccacgcc acagtttatt gtgcagctac
    12121 cacagatcct ggacttactg catcgtctga attctccatg ggcagaacaa gcccgacagt
    12181 tggttgatgc taacagcacg atcacttcag cgttacacac gctttttctc cagcgttggc
    12241 gtttaagtct gatcgtgcaa gcaacgacgt taaatcaaca gctattagaa gaagaacgcg
    12301 aacaactgtt gagtgaagtt caggaacgca tgacgctgag cggacaactt gaaccgattc
    12361 tcgcagataa caatactgca gctggtcgtc tgtgggatat gagcgccggt cagcttaaac
    12421 gtggcgacta tcagttgatt gtgaaatacg gtgaatttct taacgaacag ccggaactga
    12481 aacgcctggc agagcagctg gggcgttctc gggaagccaa atcaataccg cgcaacgatg
    12541 cgcagatgga aaccttccgc accatggtgc gcgaaccggc gacggttcct gagcaggttg
    12601 atggtctgca acaaagcgat gatattttac gtcttctgcc gccagaactg gcgacactag
    12661 ggataacgga actggagtat gagttttacc gtcggctggt ggaaaaacag ttgctcacct
    12721 atcgcctgca cggtgagtcg tggcgtgaaa aagtgatcga acgtccggtg gtacataaag
    12781 attacgatga acagccgcgc gggccgttta ttgtctgtgt ggatacttcc ggctcaatgg
    12841 gcggctttaa tgaacagtgt gcgaaagcgt tctgcctggc cttgatgcgc attgctctcg
    12901 ctgaaaaccg gcgctgctat attatgctat tttccaccga gatcgtccgt tatgagcttt
    12961 caggcccaca aggcatcgaa caagcaatcc gttttttaag ccagcagttt cgtggcggca
    13021 ccgatcttgc cagttgtttt cgcgccatta tggaacgctt gcaaagcagg gaatggtttg
    13081 atgccgatgc ggtggtgatt tctgatttta tcgctcagcg gttgcctgac gacgtgacga
    13141 gtaaagtgaa agagctgcag cgggtacatc agcatcgctt tcatgccgtg gcgatgtcgg
    13201 cacacggcaa acccggcatc atgcgcattt tcgatcatat ctggcgcttt gataccggga
    13261 tgcgaagccg cctgctcaga cgctggcggc gataaattat tacagcagag aagggacgct
    13321 ctcgcgaaca gcagctggcc atactccaca ctgaacctgg ccgatatgcg gcagttgcag
    13381 cagcagcatg gtcaaacgag actggccgat accgccgccg atggtctgcg gcatttcacc
    13441 gcgcagcagc gcctgatgcc attccagctg cagacgatct tcatcaccgg tcagcgccaa
    13501 ctgatgcttc aacgtgtcgg catccacgcg gatccccatg gaagaaagct caaacgcatc
    13561 ttccagtaca gggttccata ccagaatatc gccgttcaga cccgcatggc ccagctctga
    13621 cggggtgctc cagtcatcat aatccggtgc gcgcacgtcg tgacgatgac catcactcag
    13681 cttgccgcca atcccgacga ggaataccgc gccaagatct ttcgcaatcg cccgctcacg
    13741 ccctttggca tcgagatccg gataacgaga cagtaactcc tggctgtgta cgaagtggat
    13801 ctgatccggc aggaacggtg ccaaaccaaa ctcttcgcta actgcagctt cggttgcttt
    13861 aattcccgcc cagatcgcct ctaccgtgct tttcagagtc gagaattgac gctcaccgtc
    13921 gcccattacg cgttcccagt cccactggtc aacatagacc gagtgcaacg gagaaagacg
    13981 ctcttcatcg gggcgaaggg ctttcatgtg cgtgtacagc ccttcgcccg cgctgaagtc
    14041 gtgttgccct aaggtctgac gtttccactt cgccagtgaa tgaaccactt cgaactgggc
    14101 atcaggcaga gctttcactt ttacctgcac cgctttttca cagcccgaca agttatcctg
    14161 cgtgccatcc cccacacggc taagaatcgg cgcctggact tcgatcagcc ccagacgttc
    14221 ttccagttgg cgagaaaagt gagatttcac gaagctaatt tggcgttgtt tggcaatgta
    14281 agcggttttc attttttata ctcctgcgtc ctgttgctta tgattaagca acaaaaaccg
    14341 acacacatgc aataatcatt caataaaaag cctaacacac ttttgattca ttaaaaaaga
    14401 aggctaaaat agaatgaatc atcaatctgc ataagaaaat cctatggaaa attatctgat
    14461 cgacaatctg gaccgtggca tcctggaagc attaatgggc aatgcgcgca ccgcttacgc
    14521 cgaactggcg aaacaatttg gcgtcagtcc ggggacgatt cacgttcgag tagagaaaat
    14581 gaagcaggcg gggatcatta ccggggcgcg tattgatgtc agcccgaagc agctcggtta
    14641 tgacgtaggc tgctttatcg gcattatatt aaagagcgcc aaagactacc cttccgcgct
    14701 ggcaaagctg gaaagccttg atgaagtcac tgaagcctat tacacaaccg gccactacag
    14761 catctttata aaagtgatgt gccgttcgat cgacgctctc cagcatgtac ttatcaacaa
    14821 gatccaaaca attgatgaaa ttcagtccac cgagacattg atcgtcctgc aaaacccgat
    14881 catgcgtacc atcaagccat aatcggcttt tttaatccca tacttttcca caggtagatc
    14941 ccaacgcgtt cacagcgtac aatacgccac tcttaataaa ggtggcggtt tatggcagat
    15001 atcactctta tcagcggcag caccctcggc ggtgccgaat atgtagcaga acacctggct
    15061 gaaaagctgg aagaggcggg ttttaccacc gaaacgctgc acggtccgct gttggaagat
    15121 ttatctgcct cggggatctg gctgattatc agctccaccc acggtgccgg agatattccg
    15181 gacaaccttt ctcctttcta tgaagcattg caggaacaga agcccgatct ttctgcggtc
    15241 cgctttggcg caatcggtat tggtagtcgt gaatatgaca ccttttgtgg ggctatcgat
    15301 aaactcgagg cagaactcaa aaattccggt gcaaaacaga caggcgaaac actgaagatc
    15361 aacattcttg atcacgacat tccggaagat ccggcagaag aatggctggg atcgtggatt
    15421 aatttactca aataagtata cagatcgtgc gatctactgt ggataactct gtcaggaagc
    15481 ttggatcaac cggtagttat ccaaggaaca acggttgttc agtttttgag ttgtgtataa
    15541 cccctcattc tgatcccagc ttatacggtc caggatcacc gatcattcac agttaatgat
    15601 cctttcccgg ttgttgatct taaaagccgg atccttgtta tccacaggga gtgcgatcct
    15661 aataagagat cacaatagaa cagatctcta aataaataga tcttcttttt aatacccagg
    15721 atcccaggtc tttctcaagc cgacaaagtt gagtagaatc cacggcccgg gcttcaatcc
    15781 attttcatac cgcgttatgc gaggcaatca ccatgtttta tccggatcct tttgacgtca
    15841 tcatcattgg cgggggtcat gcaggcaccg aggccgcgat ggccgcggcg cgtatgggtc
    15901 aacagactct gcttttgaca cacaatatcg acactctggg gcagatgagc tgcaacccgg
    15961 cgatcggcgg tattgggaag ggacatctgg taaaagaagt ggatgcactc ggcggtctga
    16021 tggcgaaagc gatcgatcaa gcgggtatcc agtttaggat actaaacgca agcaaaggac
    16081 cggcggttcg cgctacccga gctcaggcgg atcgtgtgct ctaccgtcag gcggtacgta
    16141 cggcgctgga gaaccaaccg aacctgatga tcttccagca ggcggttgaa gatcttattg
    16201 tcgaaaacga tcgcgtggtc ggtgctgtta cccaaatggg actgaagttc cgtgccaaag
    16261 ccgtcgtgct caccgttggg acgttcctcg acggtaaaat tcatatcggt ctggataatt
    16321 acagcggtgg ccgtgctggt gatccgccgt ccattccgct ttctcgccgt ttgcgtgaac
    16381 tgccgctgcg cgttggtcgt ctgaaaaccg ggacaccacc gcgtattgat gctcgaacta
    16441 tcgactttag tgtgctggcg caacagcatg gcgataaccc aatgccggta ttctcgttta
    16501 tgggcaatgc gtcccagcat ccccagcagg tgccgtgtta tatcactcat accaacgaga
    16561 aaacccatga tgtgatccgc agtaacctcg atcgtagccc aatgtatgca ggggtgatcg
    16621 aaggtgtcgg cccacgctac tgcccgtcga tcgaagacaa agttatgcgc ttcgccgaca
    16681 gaaatcagca tcagatcttc cttgaaccgg aaggactgac ctctaatgaa atttatccga
    16741 acggtatctc caccagcctg ccgttcgatg tgcagatgca aatcgtccgc tctatgcagg
    16801 ggatggaaaa cgcgaagatc gtgcgtccgg gttatgccat tgagtatgac ttcttcgatc
    16861 ctcgcgacct gaaaccgacg ctggagagca agtttatcca ggggctgttc tttgctggtc
    16921 agattaacgg cactaccggt tacgaagaag ccgctgcgca aggtttgctg gccggtctta
    16981 acgctgcccg tctgtctgct gacaaagaag gttgggctcc ggcacgttct caggcgtatc
    17041 tcggcgtact agttgatgac ctgtgcactt taggaaccaa agaaccgtat cgtatgttta
    17101 cttcgcgcgc agaatatcgt ctgatgctac gcgaagataa tgcggatctg cgtttgactg
    17161 aaatcggtcg tgaactgggc ctggtggatg acgaacgttg ggcgcgcttt aacgagaaac
    17221 ttgagaatat cgagcgtgag cgtcagcgtc tgaaatcgac ctgggtaacc ccgtcggcgg
    17281 aagctgcagc cgaagtgaat gctcacctga ctgcgccgct ttcccgtgaa gccagtggtg
    17341 aagatctgct gcgtcgtccg gaaatgactt atgaaaaatt aaccacgctg acgccgtttg
    17401 cccctgcgtt gacagacgaa caggcggcgg aacaggttga gattcaggtt aaatacgaag
    17461 gttatatcgc gcgccagcaa gatgagatcg aaaagcagct gcgtaacgag aacaccctgc
    17521 tacccgcgac actggattac cgccaggtat ccggtctttc taacgaagtg atcgccaaac
    17581 ttaacgatca caaaccagcc tctatcggcc aggcttcgcg tatttctggc gtcacgcctg
    17641 cggccatctc cattctgctg gtgtggctga aaaaacaggg tatgctgcgt cgtagcgcat
    17701 aacgcattaa aaatgcctgg taagcacccg cttaccaggc aacgcatcaa gaacaggtaa
    17761 tcaccgtgct caacaaactc tccttactgc tgaaagacgc aggtatttcg cttaccgatc
    17821 accagaaaaa ccagcttatt gcctacgtga atatgctgca taaatggaac aaagcgtaca
    17881 acctgacttc ggtccgcgat cctaatgaga tgctggtacg ccatattctc gatagcattg
    17941 tggtggcacc gtatctgcaa ggtgaacggt ttatcgatgt cggcaccgga ccaggactgc
    18001 caggcattcc actctctatc gtgcgtcctg aagcccattt cactctgttg gatagccttg
    18061 gtaaacgcgt gcgtttcctt cgtcaggtgc aacatgagct taaactggag aatattgaac
    18121 cagtacagag cagggtagaa gagtttcctt cagagccgcc atttgatggc gtaattagcc
    18181 gcgcttttgc ctctctgaac gatatggtga gctggtgcca ccatcttcct ggtgagcaag
    18241 gccgtttcta cgcgctgaaa gggcaaatgc cggaagatga aatcgctttg ttgcccgaag
    18301 aatatcaggt cgaatcagtg gttaaacttc aggttccagc cttggatggc gaacgtcatc
    18361 tggtggtgat taaagcaaat aaaatttaat ttttatcaaa aaaatcataa aaaattgacc
    18421 ggttagactg ttaacaacaa ccaggttttc tactgatata actggttaca tttaacgcca
    18481 cgttcactct tttgcatcaa caagataacg tggctttttt tggtaagcag aaaataagtc
    18541 attagtgaaa atatcagtct gctaaaaatc ggcgctaaga a
//
LOCUS       DAZKHF020000072        18517 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2201S31241
            SAMN43267800-rid25106413.denovo.072, whole genome shotgun sequence.
ACCESSION   DAZKHF020000072 DAZKHF020000000
VERSION     DAZKHF020000072.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267800
            Sequence Read Archive: SRR30307263
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 18517)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18517)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 18517)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350581.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350581.2
            Genome Coverage       :: 151x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:21:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,652
            CDSs (total)                      :: 5,557
            Genes (coding)                    :: 5,320
            CDSs (with protein)               :: 5,320
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 237
            CDSs (without protein)            :: 237
            Pseudo Genes (ambiguous residues) :: 0 of 237
            Pseudo Genes (frameshifted)       :: 102 of 237
            Pseudo Genes (incomplete)         :: 130 of 237
            Pseudo Genes (internal stop)      :: 52 of 237
            Pseudo Genes (multiple problems)  :: 41 of 237
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18517
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267800-rid25106413.denovo.072"
                     /serotype="O 26:H11"
                     /isolate="2201S31241"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-01"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            101..1321
                     /locus_tag="ACHOSC_RS10275"
                     /old_locus_tag="pgaptmp_002055"
     CDS             101..1321
                     /locus_tag="ACHOSC_RS10275"
                     /old_locus_tag="pgaptmp_002055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoadenosine phosphosulfate reductase"
                     /protein_id="HGU4547995.1"
                     /translation="MSIYKIPLPLNILEAARERITWTLNTLPRVCVSFSGGKDSGLML
                     HLTAELARQMGKKICVLFIDWEAQFSCTINYVQSLRELYTDVIEEFYWVTLPLTTQNS
                     LSQYQPEWQCWEPDVEWVRQPPQDAITDPDFFCFYQPGMTFEQFVREFAEWFSQKRPA
                     AMMIGIRADESYNRFVAIASLNKQRFADDKPWTTAAPGGHSWYIYPIYDWKVADIWTW
                     YANHQNLCNPLYNLMYQAGVPLRHMRICEPFGPEQRQGLWLYHVIEPDRWAAMCARVS
                     GVKSGGIYAGHDNHFYGHRKILKPEHLDWQEYALLLLNSMPEKTAEHYRNKIAIYLHW
                     YQKKGIEVPQTQQGDIGAKDIPSWRRICKVLLNNDYWCRALSFSPTKAKNYQRYNERI
                     KGKRQEWGILCNND"
     gene            1294..1923
                     /locus_tag="ACHOSC_RS10280"
                     /old_locus_tag="pgaptmp_002056"
     CDS             1294..1923
                     /locus_tag="ACHOSC_RS10280"
                     /old_locus_tag="pgaptmp_002056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA];
                     GO_process: GO:0043170 - macromolecule metabolic process
                     [Evidence IEA];
                     GO_process: GO:0043412 - macromolecule modification
                     [Evidence IEA];
                     GO_process: GO:0044238 - primary metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IbrB-like domain-containing protein"
                     /protein_id="HGU4547996.1"
                     /translation="MGDTMQQRLTQDLTQFLASLPEDDRIKAINEIRMAIHQVSPFRE
                     EPVDCVLWVKNSQLMPNDYNPNNVAPPEKKLLKKSIEIDGFTQPIVVTHTDKNALEIV
                     DGFHRHEIGKGSSSLKLRLKGYLPVTCLEGTRNQRIAATIRHNRARGRHQITAMSEIV
                     RELSQLGWDDNKIGKELGMDSDEVLRLKQINGLQELFADRQYSRAWTVK"
     gene            complement(1924..3084)
                     /gene="ybdL"
                     /locus_tag="ACHOSC_RS10285"
                     /old_locus_tag="pgaptmp_002057"
     CDS             complement(1924..3084)
                     /gene="ybdL"
                     /locus_tag="ACHOSC_RS10285"
                     /old_locus_tag="pgaptmp_002057"
                     /EC_number="2.6.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine-oxo-acid transaminase"
                     /protein_id="HGU4547997.1"
                     /translation="MTNNPLIPQSKLPQLGTTIFTQMSALAQQHQAINLSQGFPDFDG
                     PRYLQERLAYHVAQGANQYAPMTGVQALREAIAQKTERLYGYQPDADSDITVTAGATE
                     ALYAAITALVRNGDEVICFDPSYDSYSPAIALSGGIVKRMALQPPHFRVDWQEFAALL
                     SERTRLVILNTPHNPSATVWQQADFAALWQAIAGHEIFVISDEVYEHINFSQQGHASV
                     LAHPQLRERAVAVSSFGKTYHMTGWKVGYCVAPAPISAEIRKVHQYLTFSVNTPAQLA
                     LADMLRAEPEHYLALPDFYRQKRDILVNALNESRLEILPCEGTYFLLVDYSEVSTLDD
                     VEFCQWLTQEHGVAAIPLSVFCADPFPHKLIRLCFAKKESTLLAAAERLRQL"
     gene            3193..4281
                     /gene="hcxA"
                     /locus_tag="ACHOSC_RS10290"
                     /old_locus_tag="pgaptmp_002058"
     CDS             3193..4281
                     /gene="hcxA"
                     /locus_tag="ACHOSC_RS10290"
                     /old_locus_tag="pgaptmp_002058"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxycarboxylate dehydrogenase HcxA"
                     /protein_id="HGU4547998.1"
                     /translation="MPHNPIRVVVGPANYFSHPGSFNHLHDFFTDEQLSRAVWIYGKR
                     AIAAAQTKLPPAFGLPGAKHILFRGHCSESDVQQLAAESGDDRSVVIGVGGGALLDTA
                     KALARRLGLPFVAVPTIAATCAAWTPLSVWYNDAGQALHYEIFDDANFMVLVEPEIIL
                     NAPQQYLLAGIGDTLAKWYEAVVLAPQPETLPLTVRLGINNAQAIRDVLLNSSEQALS
                     DQQNQQLTQSFCDVVDAIIAGGGMVGGLGDRFTRVAAAHAVHNGLTVLPQTEKFLHGT
                     KVAYGILVQSALLGQDDVLAQLTGAYQRFHLPTTLAELEVDINNHAEIDKVITHTLRP
                     VESIHYLPVTLTPDTLRAAFEKVESFKA"
     gene            complement(4291..4488)
                     /locus_tag="ACHOSC_RS10295"
                     /old_locus_tag="pgaptmp_002059"
     CDS             complement(4291..4488)
                     /locus_tag="ACHOSC_RS10295"
                     /old_locus_tag="pgaptmp_002059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012904950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbdD/YjiX family protein"
                     /protein_id="HGU4547999.1"
                     /translation="MFDSLAKAGKYLGQAAKLMIGMPDYDNYVEHMRVNHPDQTPMTY
                     EEFFRERQDARYGGKGGARCC"
     gene            complement(4670..6775)
                     /gene="cstA"
                     /locus_tag="ACHOSC_RS10300"
                     /old_locus_tag="pgaptmp_002060"
     CDS             complement(4670..6775)
                     /gene="cstA"
                     /locus_tag="ACHOSC_RS10300"
                     /old_locus_tag="pgaptmp_002060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009267 - cellular response to starvation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate/proton symporter CstA"
                     /protein_id="HGU4548000.1"
                     /translation="MNKSGKYLVWTVLSVMGAFALGYIALNRGEQINALWIVVASVCI
                     YLIAYRFYGLYIAKNVLAVDPTRMTPAVRHNDGLDYVPTDKKVLFGHHFAAIAGAGPL
                     VGPVLAAQMGYLPGMIWLLAGVVLAGAVQDFMVLFVSTRRDGRSLGELVKEEMGPTAG
                     VIALVACFMIMVIILAVLAMIVVKALTHSPWGTYTVAFTIPLAIFMGIYLRYLRPGRI
                     GEVSVIGLVFLIFAIISGGWVAESPTWAPYFDFTGVQLTWMLVGYGFVAAVLPVWLLL
                     APRDYLSTFLKIGTIVGLAVGILIMRPTLTMPALTKFVDGTGPVWTGNLFPFLFITIA
                     CGAVSGFHALISSGTTPKMLANEGQACFIGYGGMLMESFVAIMALVSACIIDPGVYFA
                     MNSPMAVLAPAGTADVVASAAQVVSSWGFSITPDTLNQIASEVGEQSIISRAGGAPTL
                     AVGMAYILHGALGGMMDVAFWYHFAILFEALFILTAVDAGTRAARFMLQDLLGVVSPG
                     LKRTDSLPANLLATALCVLAWGYFLHQGVVDPLGGINTLWPLFGIANQMLAGMALMLC
                     AVVLFKMKRQRYAWVALVPTAWLLICTLTAGWQKAFSPDAKVGFLAIANKFQAMIDSG
                     NIPSQYTESQLAQLVFNNRLDAGLTIFFMVVVVVLALFSIKTALAALKDPKPTAKETP
                     YEPMPENVEEIVAQAKGAH"
     gene            complement(6956..7369)
                     /gene="entH"
                     /locus_tag="ACHOSC_RS10305"
                     /old_locus_tag="pgaptmp_002061"
     CDS             complement(6956..7369)
                     /gene="entH"
                     /locus_tag="ACHOSC_RS10305"
                     /old_locus_tag="pgaptmp_002061"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proofreading thioesterase EntH"
                     /protein_id="HGU4548001.1"
                     /translation="MIWKRHLTLDELNATSDNTMVAHLGIVYTRLGDDVLEAEMPVDT
                     RTHQPFGLLHGGASAALAETLGSMAGFMMTRDGQCVVGTELNATHHRPVSEGKVRGVC
                     QPLHLGRQNQSWEIVVFDEQGRRCCTCRLGTAVLG"
     gene            complement(7372..8118)
                     /gene="entA"
                     /locus_tag="ACHOSC_RS10310"
                     /old_locus_tag="pgaptmp_002062"
     CDS             complement(7372..8118)
                     /gene="entA"
                     /locus_tag="ACHOSC_RS10310"
                     /old_locus_tag="pgaptmp_002062"
                     /EC_number="1.3.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-dihydro-2,3-dihydroxybenzoate dehydrogenase
                     EntA"
                     /protein_id="HGU4548002.1"
                     /translation="MDFSGKNVWVTGAGKGIGYATALAFVEAGAKVTGFDQAFAQEQY
                     PFATEVMDVADAAQVAQVCQRLLAETERLDVLINAAGILRMGATDQLSKEDWQQTFAV
                     NVGGAFNLFQQTMNQFRRQRGGAIVTVASDAAHTPRIGMSAYGASKAALKSLALSVGL
                     ELAGSGVRCNVVSPGSTDTDMQRTLWVSDDAEEQRIRGFGEQFKLGIPLGKIARPQEI
                     ANTILFLASDLASHITLQDIVVDGGSTLGA"
     gene            complement(8118..8975)
                     /gene="entB"
                     /locus_tag="ACHOSC_RS10315"
                     /old_locus_tag="pgaptmp_002063"
     CDS             complement(8118..8975)
                     /gene="entB"
                     /locus_tag="ACHOSC_RS10315"
                     /old_locus_tag="pgaptmp_002063"
                     /EC_number="3.3.2.1"
                     /EC_number="6.3.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enterobactin biosynthesis bifunctional
                     isochorismatase/aryl carrier protein EntB"
                     /protein_id="HGU4548003.1"
                     /translation="MAIPKLQAYALPESHDIPKNKVDWAFEPQRAALLIHDMQDYFVS
                     FWGENCPMMEQVIANIAALRDYCKQHNIPVYYTAQPKEQSDEDRALLNDMWGPGLTRS
                     PEQQKVVDRLTPDADDTVLVKWRYSAFHRSPLEQMLKESGRNQLIITGVYAHIGCMTT
                     ATDAFMRDIKPFMVADALADFSRDEHLMSLKYVAGRSGRVVMTEELLPAPVPASKAAL
                     REVILPLLDESDEPFDDDNLIDYGLDSVRMMALAARWRKVHGDIDFVMLAKNPTIDAW
                     WKLLSREVK"
     gene            complement(8989..10599)
                     /gene="entE"
                     /locus_tag="ACHOSC_RS10320"
                     /old_locus_tag="pgaptmp_002064"
     CDS             complement(8989..10599)
                     /gene="entE"
                     /locus_tag="ACHOSC_RS10320"
                     /old_locus_tag="pgaptmp_002064"
                     /EC_number="2.5.1.-"
                     /EC_number="6.2.1.71"
                     /EC_number="6.3.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(2,3-dihydroxybenzoyl)adenylate synthase EntE"
                     /protein_id="HGU4548004.1"
                     /translation="MSIPFTRWPEEFARRYREKGYWQDLPLTDILTRHAASDSIAVID
                     GERQLSYRELNQAADNLACSLRRQGIKPGETALVQLGNVAELYITFFALLKLGVAPVL
                     ALFSHQRSELNAYASQIEPALLIADRQHALFSGDDFLNTFVTEHSSIRVVQLHNDSGE
                     HNLQDAINHPAEDFTATPSPADEVAYFQLSGGTTGTPKLIPRTHNDYYYSVRRSVEIC
                     QFTQQTRYLCAIPAAHNYAMSSPGSLGVFLAGGTVVLAADPSATLCFPLIEKHQVNVT
                     ALVPPAVSLWLQALTEGESRAQLASLKLLQVGGARLSATLAARIPAEIGCQLQQVFGM
                     AEGLVNYTRLDDSAEKIIHTQGYPMCPDDEVWVADAEGNPLPQGEVGRLMTRGPYTFR
                     GYYKSPQHNASAFDANGFYCSGDLISIDPEGYITVQGREKDQINRGGEKIAAEEIENL
                     LLRHPAVIYAALVSMEDELMGEKSCAYLVVKEPLRAVQVRRFLREQGIAEFKLPDRVE
                     CVDSLPLTAVGKVDKKQLRQWLASRASA"
     gene            complement(10609..11784)
                     /gene="entC"
                     /locus_tag="ACHOSC_RS10325"
                     /old_locus_tag="pgaptmp_002065"
     CDS             complement(10609..11784)
                     /gene="entC"
                     /locus_tag="ACHOSC_RS10325"
                     /old_locus_tag="pgaptmp_002065"
                     /EC_number="5.4.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008909 - isochorismate synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isochorismate synthase EntC"
                     /protein_id="HGU4548005.1"
                     /translation="MDTSLAEEVQQTMATLAPNRFFFMSPYRSFTTSGCFARFDEPAV
                     NGDSPDSPFQQKLAALFADAKAQGIKNPVMVGAIPFDPRQPSSLYIPESWQSFSRQEK
                     QTSARRFTRSQSLNVVERQAIPEQTTFEQMVARAAALTATPQVDKVVLSRLIDITTDA
                     AIDSGVLLERLIAQNPVSYNFHVPLADGGVLLGASPELLLRKDGERFSSIPLAGSARR
                     QPDEVLDREAGNRLLASEKDRHEHELVTQAMKEVLRERSSELHVPSSPQLITTPTLWH
                     LATPFEGKANSQENALTLACLLHPTPALSGFPHQAATQVIAELEPFDRELFGGIVGWC
                     DSEGNGEWVVTIRCAKLRENQVRLFAGAGIVPASSPLGEWRETGVKLSTMLNVFGLH"
     gene            12153..13109
                     /gene="fepB"
                     /locus_tag="ACHOSC_RS10330"
                     /old_locus_tag="pgaptmp_002066"
     CDS             12153..13109
                     /gene="fepB"
                     /locus_tag="ACHOSC_RS10330"
                     /old_locus_tag="pgaptmp_002066"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe2+-enterobactin ABC transporter
                     substrate-binding protein"
                     /protein_id="HGU4548006.1"
                     /translation="MRLAPLYRNALLLTGLLLSGIAAVQAADWPRQITDSRGTHTLES
                     QPQRIVSTSVTLTGSLLAIDAPVIASGATTPNNRVADDQGFLRQWSKVAKERKLQRLY
                     IGEPSTEAVAAQMPDLILISATGGDSALALYDQLSTIAPTLIINYDDKSWQSLLTQLG
                     EITGHEKQAAERIAQFDKQLAAAKEQIKLPPQPVTAIVYTAAAHSANLWTPESAQGQM
                     LEQLGFTLAKLPAGLNASQSQGKRHDIIQLGGENLAAGLNGESLFLFAGDQKDADAIY
                     ANPLLAHLPAVQNKQVYALGTETFRLDYYSATQVLERLKALF"
     gene            complement(13113..14363)
                     /gene="entS"
                     /locus_tag="ACHOSC_RS10335"
                     /old_locus_tag="pgaptmp_002067"
     CDS             complement(13113..14363)
                     /gene="entS"
                     /locus_tag="ACHOSC_RS10335"
                     /old_locus_tag="pgaptmp_002067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042931 - enterobactin transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0042930 - enterobactin transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enterobactin transporter EntS"
                     /protein_id="HGU4548007.1"
                     /translation="MNKQSWLLNLSLLKTHPAFRAVFLARFISIVSLGLLGVAVPVQI
                     QMMTHSTWQVGLSVTLTGGAMFVGLMVGGVLADRYERKKVILLARGTCGIGFIGLCLN
                     ALLPEPSLLAIYLLGLWDGFFASLGVTALLAATPALVGRENLMQAGAITMLTVRLGSV
                     ISPMIGGLLLATGGVAWNYGLAAAGTFITLLPLLSLPALPPPPQPREHPLKSLLAGFR
                     FLLASPLVGGIALLGGLLTMASAVRVLYPALADNWQMSAAQIGFLYAAIPLGAAIGAL
                     TSGKLAHSARPGLLMLLSTLGSFLAIGLFGLMPMWILGVVCLALFGWLSAVSSLLQYT
                     MLQTQTPEAMLGRINGLWTAQNVTGDAIGAALLGGLGAMMTPVASASASGFGLLIIGV
                     LLLLVLVELRRFRQTPPQVTASDS"
     gene            14474..15478
                     /gene="fepD"
                     /locus_tag="ACHOSC_RS10340"
                     /old_locus_tag="pgaptmp_002068"
     CDS             14474..15478
                     /gene="fepD"
                     /locus_tag="ACHOSC_RS10340"
                     /old_locus_tag="pgaptmp_002068"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe(3+)-siderophore ABC transporter permease"
                     /protein_id="HGU4548008.1"
                     /translation="MSGSVAVTRAIAVPGLLLLLIIATALSLLIGAKSLPASVVLEAL
                     SGTCQSADCTIVLDARLPRTLAGLLAGGALGLAGALMQTLTRNPLADPGLLGVNAGAS
                     FAIVLGAALFGYSSAQEQLAMAFAGALVASLIVAFTGSQGGGQLSPVRLTLAGVALAA
                     VLEGLTSGIALLNPDVYDQLRFWQAGSLDIRNLHTLKVVLIPVLIAGATALLLSRALN
                     SLSLGSDTATALGSRVARTQLIGLLAITVLCGSATAVVGPIAFIGLMMPHMARWLVGA
                     DHRWSLPVTLLATPALLLFADIIGRVIVPGELRVSVVSAFIGAPVLIFLVRRKTRGGA
                     "
     gene            15475..16467
                     /gene="fepG"
                     /locus_tag="ACHOSC_RS10345"
                     /old_locus_tag="pgaptmp_002069"
     CDS             15475..16467
                     /gene="fepG"
                     /locus_tag="ACHOSC_RS10345"
                     /old_locus_tag="pgaptmp_002069"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-enterobactin ABC transporter permease"
                     /protein_id="HGU4548009.1"
                     /translation="MIYVSRRLIITCLLLVSACVVAGIWGLRSGAVTLETSQVFAALM
                     GDAPRSMTMVVTEWRLPRVLMALLIGAALGVSGAIFQSLMRNPLGSPDVMGFNTGAWS
                     GVLVAMVLFGQDLTAIALAAMVGGIITSLLVWLLAWRNGIDTFRLIIIGIGVRAMLVA
                     FNTWLLLKASLETALTAGLWNAGSLNGLTWAKTSPSAPIIILMLIAAALLVRRMRLLE
                     MGDDTACALGVSVERSRLLMMLVAVVLTAAATALAGPISFIALVAPHIARRISGTARW
                     GLTQAALCGALLLLAADLCAQQLFMPYQLPVGVVTVSLGGIYLIVLLIQESRKK"
     gene            16464..17279
                     /gene="fepC"
                     /locus_tag="ACHOSC_RS10350"
                     /old_locus_tag="pgaptmp_002070"
     CDS             16464..17279
                     /gene="fepC"
                     /locus_tag="ACHOSC_RS10350"
                     /old_locus_tag="pgaptmp_002070"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-enterobactin ABC transporter ATP-binding
                     protein"
                     /protein_id="HGU4548010.1"
                     /translation="MNESVARLRGEQLTLGYGKYTVAENLTVEIPDGHFTAIIGPNGC
                     GKSTLLRTLSRLMTPAHGHVWLDGEHIQHYASKEVARRIGLLAQNATTPGDITVQELV
                     ARGRYPHQPLFTRWRKEDEEAVTKAMQATGITHLADQSVDTLSGGQRQRAWIAMVLAQ
                     ETAIMLLDEPTTWLDISHQIDLLELLSELNREKGYTLAAVLHDLNQACRYASHLIALR
                     EGKIVAQGAPKEIVTAELIERIYGLRCMIIDDPVAGTPLVVPLGRTAPSTANS"
     gene            complement(17276..18409)
                     /gene="wzz(fepE)"
                     /locus_tag="ACHOSC_RS10355"
                     /old_locus_tag="pgaptmp_002071"
     CDS             complement(17276..18409)
                     /gene="wzz(fepE)"
                     /locus_tag="ACHOSC_RS10355"
                     /old_locus_tag="pgaptmp_002071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS O-antigen length regulator Wzz(fepE)"
                     /protein_id="HGU4548011.1"
                     /translation="MSSLNIKQGSDAHFPEYPLASPSNNEIDLLNLIAVLWRAKKTVM
                     AVIFAFACAGLLISFILPQKWTSAAVVTPPEPVQWQELEKTFTKLRVLDLDIKIDRTE
                     AFNLFIKKFQSVSLLEEYLRSSPYVMDQLKEAKIDELDLHRAIVALSEKMKAVDDNAS
                     KKKDEPSLYTSWTLSFTAPTSEEAQTVLSGYIDYISALVVKESLENVRNKLEIKTQFE
                     KEKLAQDRIKTKNQLDANIQRLNYSLDIANAAGIKKPVYSNGQAVKDDPDFSISLGAD
                     GIERKLEIEKAVTDVAELNGELRNRQYLVEQLTKANINDVNFTPFKYQLSPSLPVKKD
                     GPGKAIIVILSALIGGMVACGSVLLRYAMASRKQDAMMADHLV"
BASE COUNT         4356 a         5243 c         4786 g         4132 t
ORIGIN      
        1 gcgcgaatga aaaataaaac actctgcttt gcagtgttta ctttataatt aacagactta
       61 tctcactgat caccctgtaa cgttcagaga gcgtcttcag atgtctattt ataaaattcc
      121 tcttccgctc aatattctgg aagctgcacg agagcgtatt acctggacac tcaacaccct
      181 accccgcgtt tgtgtctcat tttctggcgg caaagactct ggtttaatgc tgcatctgac
      241 agccgaactt gcccgacaaa tgggcaaaaa aatctgcgtt ttgtttatcg actgggaggc
      301 gcaattctct tgcaccatta actatgttca gtccctgcgc gagttgtaca ccgatgtcat
      361 cgaagagttt tactgggtta cgctcccgct tacgacgcaa aattcccttt cacaatacca
      421 acccgaatgg cagtgctggg aacctgatgt cgaatgggtg cgtcagcccc cgcaagatgc
      481 gataaccgat cctgactttt tctgctttta ccagccaggc atgaccttcg aacaatttgt
      541 acgtgagttt gccgaatggt tttcacaaaa acgtccggcg gcgatgatga tcggcatccg
      601 tgcggatgag tcctacaacc gttttgtcgc catcgccagt ttaaataaac aacgttttgc
      661 cgacgataaa ccatggacta cggccgcacc aggcggtcat agctggtaca tttaccccat
      721 ttacgactgg aaagtggctg atatctggac ctggtatgca aatcatcaaa atctctgcaa
      781 cccactgtat aacctaatgt atcaggcagg cgttcctctg cgtcatatgc gaatttgcga
      841 accttttggc ccggagcaac gacaaggatt atggctctat cacgttatcg aaccggatcg
      901 ctgggctgct atgtgcgcac gagtcagcgg ggtaaaaagt ggcggcattt acgccggaca
      961 tgataatcat ttctatgggc atcggaaaat cctcaagcca gaacatttag actggcaaga
     1021 atatgcatta ttgctgctca atagcatgcc ggaaaaaaca gctgagcatt accgcaataa
     1081 aatcgccatt tatttgcact ggtatcagaa aaaaggcatc gaggttccac aaacccagca
     1141 aggggacatt ggcgcgaaag atatcccctc ctggcggcgg atatgcaaag ttttactcaa
     1201 taacgattac tggtgtcggg cattatcatt tagtcctacg aaagcgaaga actatcagcg
     1261 ttataacgaa cggataaaag gaaaacgtca ggaatggggg atactatgca acaacgatta
     1321 acgcaggatt taacgcaatt tctcgccagc ttgccggaag atgatcgcat caaagcgatt
     1381 aatgaaatcc gcatggcgat ccatcaggtt agcccctttc gtgaagagcc agtggattgc
     1441 gttctgtggg ttaaaaacag ccaacttatg cctaatgatt acaacccaaa caacgtggca
     1501 ccacccgaaa aaaaactgct gaaaaaatcc attgaaatag atggttttac ccaacccatt
     1561 gtggtcacgc atacggataa aaatgccctg gaaatcgtgg atggttttca ccgccatgaa
     1621 attggtaaag gctcatcatc attaaaatta cgcttaaaag gttatttgcc ggtgacctgt
     1681 ctggaaggta cgcgtaatca acgcatcgcc gcgactattc gtcataatcg cgctcgtgga
     1741 cgccatcaaa tcaccgccat gtcggaaatc gtccgtgaac ttagccagtt gggatgggac
     1801 gataataaaa ttggcaaaga gctaggaatg gacagcgacg aagtgctgcg cctgaagcaa
     1861 attaacggcc tgcaagagct gtttgccgac cgtcaatatt cccgcgcctg gacggttaaa
     1921 taactaaagc tggcgcaggc gttcagctgc tgccagcaac gtcgattcct tcttggcaaa
     1981 acagagacga atcagtttat gtgggaaggg atcggcgcaa aacaccgaca gcggaatcgc
     2041 cgctacgccg tgctcctgcg tcagccactg gcaaaactca acatcatcca gggtagaaac
     2101 ctcgctgtaa tccaccagca aaaagtatgt accttcacac ggtaaaatct ccagccggct
     2161 ttcatttaaa gcattcacca gaatatcgcg cttctggcga taaaagtccg gtaacgcaag
     2221 ataatgctca ggttctgcac gtagcatatc agcaagcgcc agctgtgccg gggtattcac
     2281 cgaaaaggtc agatactgat gtaccttgcg aatttcggcg ctgatgggcg ctggcgcaac
     2341 acaataaccc actttccagc cggtcatatg ataggtcttg ccaaatgaag aaaccgccac
     2401 tgcccgctca cgcagctgcg gatgcgccag cacactggca tggccctgtt gtgaaaagtt
     2461 gatgtgctcg tagacttcat cgctaatgac aaaaatctcg tgcccggcga tcgcctgcca
     2521 caaagcggcg aaatcagcct gctgccagac agttgcactg gggttatgcg gagtgttgag
     2581 gatcaccagt ctggtgcgct cgcttaataa tgcggcaaat tcctgccagt caacgcgaaa
     2641 atgcggtggt tgcagtgcca tacgcttcac tattccccca gaaagcgcga tggcggggga
     2701 gtaactgtca tagctgggat caaaacaaat cacttcatcg ccattgcgca ccagtgcggt
     2761 aatcgccgcg tataacgctt ccgtcgcccc tgccgttacg gtgatatcgc tatcggcatc
     2821 tggttgatag ccatacaaac gttccgtttt ctgagcaatc gcctcgcgca aggcctgcac
     2881 gccggtcatg ggcgcgtatt ggtttgcccc ctgggcaacg tggtacgcca gccgctcctg
     2941 taaatagcgc ggaccatcaa aatcaggaaa gccttgcgac aggttaatcg cctggtgttg
     3001 ctgcgccagc gcgctcatct gggtgaaaat agtggtgcca agttgtggaa gtttgctttg
     3061 tggaatcaga gggttatttg tcatttttat tgtacctgac gaagttgcgt gttgaaggca
     3121 ctataacacg atgctagtat ttggcaatca agacgtttag atgtctaaat aaaacaataa
     3181 ggacaacaca acatgcctca caatcctatc cgcgtggtcg tcggcccggc taactacttt
     3241 tcacatccag gaagtttcaa tcacctgcac gattttttca ctgatgaaca actttctcgc
     3301 gcggtgtgga tctacggcaa acgcgccatt gctgcggcgc aaaccaaact tccgccagcg
     3361 tttggacttc caggggcaaa gcatattttg tttcgtggtc attgcagcga aagcgatgta
     3421 cagcaactgg cggctgagtc cggtgacgat cgcagcgtgg tgattggcgt cggcggcggc
     3481 gcactgctcg acaccgcgaa agccctcgcc cgccgtctcg gtctgccgtt tgttgccgtt
     3541 ccgacgatcg ccgccacctg cgccgcctgg acaccgctct ccgtctggta taacgatgcc
     3601 ggacaggcgc tgcattatga gattttcgac gacgccaatt ttatggtgct ggtggaaccg
     3661 gagattatcc tcaatgcacc gcaacaatat ctgctggcgg ggatcggtga cacgctggcg
     3721 aaatggtatg aagcggtggt gctggctccg caaccagaaa cgttgccgct aaccgtgcga
     3781 ctggggatca ataatgcgca ggccattcgc gacgtattgt taaacagtag cgaacaggcg
     3841 ctgagcgatc agcaaaatca acagttaacg caatcatttt gcgatgtggt ggatgctatt
     3901 attgctggtg gtgggatggt cggtggtctg ggcgatcgtt ttacgcgtgt ggcggcggct
     3961 catgccgtgc ataacggtct gactgtcctg ccgcaaaccg agaaatttct ccacggcacc
     4021 aaagtcgctt atggcattct ggtacaaagc gccttgctgg gtcaggatga tgtgctggcg
     4081 caattaaccg gggcgtatca gcgttttcat ctgccgacca cgctggcgga gctggaagtg
     4141 gatatcaata atcatgcgga gatcgacaaa gtgattaccc acaccctgcg tccggtggaa
     4201 tccattcatt acctgccagt cacgctgaca ccagatacgt tgcgtgcagc gttcgaaaaa
     4261 gtggaatcgt ttaaagcctg acgtgcaaat ttagcagcag cgcgcgccgc cttttccacc
     4321 gtagcgcgcg tcctgccgct cacggaaaaa ctcttcgtag gtcatcggcg tttgatcggg
     4381 atggttaacc cgcatatgtt cgacatagtt gtcgtaatca ggcataccaa tcatcagctt
     4441 cgccgcctga cctaaatatt ttccggcttt tgccagtgaa tcaaacatct tggcctccgc
     4501 taaccatgtt gctgaaagag tgccggatgc ggcgtgaacg ccttatccgg cctacaaatg
     4561 ctatcaacgc gctcggtcag tcccctcgcc ccatcgggga gagggttagg gtgaggagga
     4621 accctcggca ctatgccaac attcgccaac atcccccctc actctgactt tagtgtgcgc
     4681 cttttgcctg tgccacgatc tcctcgacat tttccggcat tggctcatac ggcgtttctt
     4741 tcgccgttgg cttcggatct ttcaatgccg ccagtgccgt cttaatcgag aacagcgcca
     4801 gaaccaccac gaccaccata aagaagatgg ttaacccggc atccagacgg ttgttgaaca
     4861 ccagttgcgc cagctgtgac tcagtatact gcgacggaat attgccgctg tcgatcattg
     4921 cctggaactt attagcaatg gccaggaagc cgactttcgc atccgggcta aacgctttct
     4981 gccagcctgc ggtcagggta caaatcagca gccaggccgt tggtaccagc gccacccagg
     5041 cgtaacgttg acgtttcatc ttgaacaaca ccacggcaca gagcatcagc gccatccctg
     5101 ccagcatctg gttggcaata ccaaacagcg gccacagagt gttaatgccg cccagcggat
     5161 cgaccacgcc ctgatggagg aagtagcccc acgccagtac gcacagcgct gttgccagca
     5221 ggttagcagg cagtgaatcg gtccgcttca ggcccggaga aaccacgccc agcagatcct
     5281 gcaacataaa gcgcgcagca cgcgtacccg catctaccgc cgtcagaata aacagagctt
     5341 caaacagaat ggcgaagtga taccagaacg ccacatccat cataccaccc agcgcaccgt
     5401 gcaggatgta agccatcccc accgccagcg tcggcgcacc gcccgcacgg gaaatgatcg
     5461 actgttcacc cacttcgctg gcaatctggt tgagcgtatc tggtgtaatg ctaaagcccc
     5521 agctactcac cacctgcgcg gcagaagcga ctacatccgc cgtccctgcc ggagccagca
     5581 ccgccattgg gctgttcatg gcaaaataca cgcccggatc gatgatacag gcagaaacca
     5641 gcgccataat cgccacgaag gattccatca acatcccacc gtagccgata aagcacgcct
     5701 gcccttcgtt cgccagcatc ttcggcgtgg tgccggaaga gatcagcgca tggaagccag
     5761 acaccgcacc acaggcgatg gtgataaaca ggaacgggaa caggttaccg gtccataccg
     5821 ggccagtgcc atcgacaaat ttggtcagcg caggcatggt cagcgtcggg cgcataatca
     5881 aaatgcctac cgccagaccg acgatagtcc cgattttcag gaaggtcgag agatagtcac
     5941 gcggggccag cagcaaccac accggcagca ccgccgccac aaaaccgtag cccaccagca
     6001 tccaggtcag ctgcacgccg gtaaagtcaa agtacggtgc ccaggtcgga ctttctgcca
     6061 cccagccgcc agagataatg gcgaaaatca ggaacaccag gccaatcact gacacttcgc
     6121 caatgcggcc cggacgcaga tagcgcaggt agattcccat aaaaatcgcc agcggaatgg
     6181 tgaacgcaac ggtatacgtt ccccacgggc tatgagtcag ggctttcacc acgatcatcg
     6241 ccagtactgc aaggataatg accatgatca taaagcaggc taccagcgca atcaccccgg
     6301 cagttggccc catctcttct ttgaccagct cacccagcga tcgaccgtca cggcgcgtag
     6361 aaacaaacag caccatgaaa tcctgcaccg caccagcgag aaccaccccg gcgagcagcc
     6421 agatcatccc tggcaggtaa cccatttgcg ccgccagtac cggcccaacc aacggacctg
     6481 ctccggcaat ggccgcaaaa tggtgaccga acagcacttt cttgtccgtc ggcacatagt
     6541 ccagcccgtc gttatggcgc accgctggcg tcatacgcgt cgggtcaacc gccagcacat
     6601 ttttggcgat atacagccca taaaaacggt aagcgatcag atagatacag accgacgcca
     6661 ccacaatcca cagcgcgttg atctgttccc cacgatttaa agcaatgtat cccagagcaa
     6721 atgctcccat tacagagagc actgtccaga cgaggtattt ccctgatttg ttcatagttg
     6781 ttatccgtgt gcgtgtccat agagatgtta cattttgttt ctataacatc tttgtaagat
     6841 ttaacaacac ggtaaccatg taaatcggag ttgaaaccaa tatttaactt aacaatgtta
     6901 actcgatcac tccgttaacc gagttttcct gcaatctcat ttaattctgt cgggctcatc
     6961 ccaaaactgc cgtacccagc cgacaagtgc agcaacgccg cccctgttca tcgaaaacga
     7021 cgatttccca gctctgattt tgccgcccaa gatgcagcgg ctggcagacg ccgcgtacct
     7081 tgccttcaga caccgggcga tgatgggtgg cgttaagttc cgtacccacc acacactgcc
     7141 cgtcacgggt catcataaat ccggccatcg atcccagcgt ttcagccagc gccgccgacg
     7201 cgccgccatg cagcaggcca aacggctgat gagtacgggt atcaaccggc atttcggctt
     7261 ccagcacatc atctcccaga cgggtataca caattcccag atgcgccacc attgtgttat
     7321 cgctggtggc gttcagttcg tcgagcgtta aatggcgttt ccagatcatg cttatgcccc
     7381 cagcgttgag ccgccatcga ccacaatatc ctgtagggtg atatggctgg cgaggtcaga
     7441 ggcgaggaac aaaatcgtgt tggcgatctc ttgtggacgg gcgattttcc ccagcggaat
     7501 gccgagttta aactgctcgc caaagccgcg aatacgctgt tcttcggcgt catcgctcac
     7561 ccacagcgtg cgttgcatat cggtgtcggt ggagccaggc gaaaccacat tacagcgcac
     7621 gccgctaccc gccagttcca gcccgacgct caacgccagg cttttcagcg ccgctttcga
     7681 tgcgccataa gcactcatac caatacgcgg cgtgtgtgcg gcgtcggacg ccacagtgac
     7741 aatcgccccg ccccgctgac ggcgaaactg gttcatggtt tgctggaaca ggttaaacgc
     7801 accgccgacg ttaaccgcaa aagtctgctg ccagtcctct ttactgagct gatcggtcgc
     7861 gcccatgcgc aaaattcccg ccgcattgat cagcacgtcc agtcgctccg tttcagctaa
     7921 cagtcgctga catacctgcg cgacctgcgc agcatcggca acatccatca cttcggtcgc
     7981 aaagggatat tgctcctgag cgaacgcttg atcaaaaccg gtaactttcg ctcccgcctc
     8041 aacaaacgcc agcgccgtgg cgtagccgat acctttacct gcgccggtta cccagacatt
     8101 tttaccgctg aaatccatta tttcacctcg cgggagagta gcttccacca ggcgtcgatg
     8161 gtcgggtttt tcgccagcat gacaaagtcg atatcaccat gcactttgcg ccagcgcgcc
     8221 gccagcgcca tcatgcgcac cgaatccaga ccgtagtcga tcaggttgtc gtcatcgaac
     8281 ggttcatcgg actcgtccag caacggcagg ataacctcac gcagcgccgc tttgctggcg
     8341 gggacaggtg ctggcagtaa ttcttcagtc atcaccaccc ggccagaccg cccggccaca
     8401 tatttcagcg acatcaaatg ctcgtcacgg ctgaaatcgg ccagcgcatc cgccaccata
     8461 aacggtttaa tatcgcgcat aaatgcgtcg gttgcggtgg tcatacagcc aatgtgggca
     8521 tataccccgg taataatcag ctggttacgt ccactctctt tgagcatttg ctccagcgga
     8581 gaacgatgaa acgcgctgta gcgccacttc accagcaccg tgtcgtcggc atctggcgtc
     8641 aggcgatcca ccaccttttg ctgttccggc gaacgggtca ggcccggccc ccacatatca
     8701 ttcaacagcg cccgatcttc atcgctctgc tctttcggct gggcggtgta ataaaccgga
     8761 atattgtgct gtttgcagta gtcgcgcagc gcagcaatat tcgcgatcac ctgctccatc
     8821 atcgggcagt tctcgcccca gaagctgaca aaatagtcct gcatatcatg gattaacaac
     8881 gcggcacgtt gcggttcaaa ggcccagtca actttattct tagggatatc gtgagactcc
     8941 ggcagtgcgt aagcctgtaa ttttggaata gccatcgtgt tctctccttc aggctgatgc
     9001 gcgtgacgcc agccactgac gtaattgttt tttatcgact ttcccgaccg ccgtcagcgg
     9061 aagtgaatct acacactcca cgcgatccgg taatttaaat tcggcaatac cctgttcacg
     9121 caggaaacga cgcacctgca ccgcgcgcag cggctctttt accaccagat aagcgcagct
     9181 tttttcgccc atcagctcat cttccatgct caccagtgcg gcgtagatca ccgccgggtg
     9241 gcgcagcagc aggttttcga tctcttcggc agcgatcttc tcaccgccac ggttgatctg
     9301 atctttctcg cgcccctgca cggtgatgta cccctctgga tcaatagaga tcagatcgcc
     9361 ggaacagtaa aaaccgttgg catcaaaggc gctggcattg tgctgcgggc ttttgtaata
     9421 gccacggaag gtgtacggac cgcgggtcat caggcgtcca acttcccctt gcggcagtgg
     9481 atttccttcg gcatcggcaa cccacacttc atcgtccgga cacattgggt aaccctgggt
     9541 atggataatt ttctccgcgc tatcatcaag acgggtgtag ttcaccagcc cttccgccat
     9601 gccaaacacc tgctgcaact ggcagccaat ctcagcggga atacgcgccg caagggtggc
     9661 agaaagacgt gcgccgccga cctgtaacag tttcagcgag gcaagctgcg cccggctttc
     9721 accttcggtc agcgcctgca accacaggct gactgccggt ggcaccagcg cggtgacgtt
     9781 cacctgatgt ttttcaatca atgggaagca aagcgtggcg ctgggatcgg ccgccagaac
     9841 aaccgttcct ccggcaagaa agacgcccag cgatcccggc gaactcatgg cgtagttatg
     9901 agccgccggg atcgcgcaca ggtagcgtgt ctgttgtgtg aactgacaaa tctcgacgct
     9961 acgacgcacg ctgtagtagt agtcgttatg agtgcgcggg atcagtttcg gtgtgccggt
    10021 ggtgccgccg gaaagctgga aataggccac ttcatcagca ggtgatggcg tggcagtaaa
    10081 atcctcagcc ggatggttaa tcgcatcctg caagttatgc tcaccgctgt cgttgtgcag
    10141 ttgcaccacg cgaatggagg aatgttctgt gacgaaagta ttgaggaaat catccccgct
    10201 aaacagcgca tgttggcgat cggcaatcag caatgcgggt tcaatctggc tggcataggc
    10261 gttcagttca ctacgctgat ggctgaacag cgccagcacc ggcgcaacgc ccagtttcag
    10321 cagcgcgaaa aaggtaatat acaattcagc gacgttaccc agttgtacca gcgcggtttc
    10381 accaggttta atgccctgac ggcgtaaact acacgcgagg ttatccgccg cctgattcag
    10441 ctcccggtaa ctcaactgtc gctcgccgtc gataaccgcg atgctgtcac tcgcagcgtg
    10501 gcgagtcaga atgtcggtca gcggcaaatc ctgccagtag cctttttccc gatagcgacg
    10561 ggcaaactct tccggccagc gggtgaatgg aatgctcatc ctcgctcctt aatgcaatcc
    10621 aaaaacgttc aacatggtag aaagtttgac gcctgtttcg cgccactcac ccaacggtga
    10681 cgacgcaggc acaatccccg ctccggcaaa cagacgcacc tgattttccc gcagcttcgc
    10741 gcagcggatg gtcaccaccc attcgccgtt accttcgctg tcacaccaac ccacaatgcc
    10801 gccaaacagt tcgcggtcga atggctccag ttcagcaata acctgggtcg cggcctgatg
    10861 cgggaaaccg ctcagcgcgg gggttggatg cagcagacag gccagagtca gtgcgttttc
    10921 ttgcgaattc gctttacctt caaagggagt tgcgagatgc cacagcgtcg gcgtggtaat
    10981 caattgtgga gaggagggaa cgtgtaactc actactgcgt tcgcgcagta cctctttcat
    11041 cgcctgagtc accagttcat gttcatggcg atctttttct gacgccagca gacgattacc
    11101 cgcttcgcga tcgagcactt catccggctg acgacgcgcg gaaccggcta acggaatgga
    11161 gctaaaacgc tcgccgtctt tacgtagcag cagttccggg ctggccccca gcaggacgcc
    11221 accatcagcc agcgggacat ggaagttgta actaaccggg ttttgcgcaa tcaaccgttc
    11281 cagcaatacg ccactatcaa tggcggcgtc agtggtgata tcaatcaatc gtgacaacac
    11341 cactttgtcg acctgcggcg tggcggtaag tgcggcggcg cgggcaacca tctgttcaaa
    11401 cgtggtttgt tcaggaattg cctggcgttc caccacgttc agcgactggc tgcgggtaaa
    11461 acggcgggct gaggtctgtt tttcctggcg ggagaacgac tgccaggatt cgggaatata
    11521 cagcgacgaa ggctgacgtg gatcgaaggg aatcgccccg accatcaccg gatttttgat
    11581 gccctgcgct ttggcatcgg caaacagcgc ggcgagtttt tgctggaagg gactgtcggg
    11641 cgaatccccg ttcacagccg gttcatcgaa gcgggcgaaa catcctgacg tcgtaaaact
    11701 gcggtacggc gacataaaga aaaagcgatt gggcgcaagt gttgccatgg tctgctgtac
    11761 ttcctcagcc agtgacgtat ccatatcatc ctccacaaaa tgataaaggc tttaataatg
    11821 attatcattt atattttcgg tcgctaacct aaaagcaaac gcgcactatg tcaactcttg
    11881 aggtaacgca atctgcaact cggggttgca tctggttgtg gttacaatga aaatgagaag
    11941 cattattgat ggattcgcat aagcgcaatg tgatgtcctg cgccgttctg ccccctctcc
    12001 cttccagggt gagggctggg gtgagggtta atgttcgcac cagtgctggc tgttcccctc
    12061 accctctccc caaaggggcg aggggacgga ttgtgcgctt tgtcgaattt gtcattacgc
    12121 ccataacctt attaataaca ggaagctgat ttgtgagact cgccccgctc taccgcaacg
    12181 cccttctatt aacaggactt ttgctttcag gaatagccgc agttcaggcc gctgactggc
    12241 cgcgtcagat tactgacagc cgtggcacac atacactgga aagccagccg cagcgtattg
    12301 tttccaccag cgtcaccctg accggctcac tgctggcgat tgatgctccg gtgatcgcca
    12361 gcggcgcgac cacgccgaat aaccgcgtcg cggatgacca gggcttttta cgccagtgga
    12421 gcaaggtggc gaaagaacgc aaactgcaac ggctctatat cggcgaaccg agcaccgaag
    12481 ccgttgccgc gcaaatgccg gatctgattt taattagcgc aaccggcggg gattcggcgc
    12541 tggcactgta tgatcagctt tccaccatcg ccccgacatt aatcatcaat tacgacgaca
    12601 aaagctggca gtcgctgtta acgcagcttg gcgaaattac cgggcatgag aaacaagcgg
    12661 cagagcggat tgcacagttt gataagcaac tggcagcggc gaaagagcaa atcaaattac
    12721 cgccgcagcc ggtcactgcc attgtctata ccgccgctgc acacagtgcc aatctctgga
    12781 cgccggaatc agcacaaggg cagatgctgg aacaactcgg ctttacgctg gcgaagttgc
    12841 ccgcagggtt aaacgccagc caaagccagg gcaaacgcca tgacatcatt cagcttggtg
    12901 gggaaaatct ggctgctggg ttaaatggcg agtcactatt cctgttcgcc ggtgatcaga
    12961 aagatgccga tgcgatttat gccaatccac tgctcgcgca ccttcccgca gtacaaaaca
    13021 agcaggttta tgcgctggga accgagacgt tccgtctgga ttactacagc gccacgcaag
    13081 tgctggaacg acttaaggcg ctgttttaag cattaactgt cagacgctgt cacctgcggc
    13141 ggcgtctggc gaaaacgtcg caactccacc agcaccagca ataacaacac gccgataatc
    13201 aacaaaccaa aaccgcttgc gcttgcggaa gcaaccggtg tcatcatcgc gcccaaacca
    13261 cccagcagcg ccgcgcctat ggcatcgccc gtcacgttct gcgccgtcca caaaccgtta
    13321 atccgcccta acatcgcttc cggggtttgc gtttgcagca ttgtatattg cagcaacgaa
    13381 ctaaccgcgc tcagccagcc gaacagcgcc agacaaacca cgcctaaaat ccacatcggc
    13441 atcaggccaa acagaccaat ggcgaggaac gatcccagcg tggagagcag catcaataac
    13501 cctggtcgcg cactatgtgc cagcttcccg ctggttaacg caccaatagc cgcgccgagc
    13561 gggatcgccg cgtagagaaa accaatctgt gccgctgaca tctgccagtt gtcagccagc
    13621 gccggataca gtacccgcac cgcgctcgcc atcgtcaata aaccacccag cagcgcaatc
    13681 ccgcccacca gcgggctggc aagcagaaaa cgaaatcctg ccagtaatga tttcaacgga
    13741 tgctcacgcg gctgcggtgg cggtggcaac gccggaaggc ttaacaacgg tagcaaggta
    13801 ataaacgtgc ccgccgccgc cagcccgtag ttccaggcta cgccaccggt cgccagcaat
    13861 aaaccgccaa tcatgggcga aatcaccgac cccagacgca cggtcaacat ggtgatcgcc
    13921 ccggcctgca ttaagttttc acgccctacc agtgctggtg tcgccgccaa tagcgccgta
    13981 acgccaagcg atgcgaaaaa accatcccat aaaccaagta aatagattgc cagcaatgac
    14041 ggctccggca gcagtgcatt aaggcacagt ccaatgaagc caatgccaca ggtgccgcgc
    14101 gccagcaaaa tcactttttt gcgctcatag cgatccgcca gcacaccgcc aaccatcagg
    14161 ccaacaaaca tcgcgccgcc ggtcagcgtc accgaaagcc ccacctgcca ggtagaatgc
    14221 gtcatcatct ggatctgcac cggcaccgcg acgccgagca aacccagaga cacaattgag
    14281 ataaaacgag cgaggaatac tgcgcgaaac gccgggtgcg ttttcaacag gctgaggtta
    14341 agcagccagg attgtttatt cattacaatg ccttgccatc aaatatttta aatacctatt
    14401 cttaggctgc acatgctaac atatccaaat aagatagata acgataatta atttcattat
    14461 catggaagtt cgtatgtctg gttctgttgc cgtgacacgc gccattgccg tgcccggatt
    14521 gctgttatta ctgattatcg cgacggcatt aagcctgctc attggggcaa aatcactccc
    14581 cgcttccgta gtgctggagg ccctctccgg cacctgccag agcgccgact gcaccatcgt
    14641 gctcgacgcg cgactgccgc gcacccttgc cggtttactt gcaggcggcg cgcttggcct
    14701 tgccggggcg ttgatgcaaa ccctcacccg aaatccactt gccgaccccg gcttgcttgg
    14761 cgtgaatgcc ggagccagct ttgccattgt gctgggtgcg gcgctgtttg gttactcatc
    14821 cgcgcaggaa caactggcga tggccttcgc cggggcgctg gtggcctcat tgattgttgc
    14881 ctttaccggc agtcagggcg gcgggcagtt aagtccggtg cgtttaaccc tggcgggcgt
    14941 ggcgctggcg gcggtgctgg aagggctgac cagcggcatc gccctgctta atcctgatgt
    15001 ctacgatcaa ctgcgtttct ggcaagccgg ttcgctggat attcgcaatc tacatacctt
    15061 aaaagtggtg ctgatcccgg tgctgatcgc cggagcaacc gcgctattac tgagccgcgc
    15121 gctgaacagt ttaagcctcg gcagcgacac cgcgacggcg ctgggcagtc gcgtggcgcg
    15181 cacacagtta attggtctgc tggcgattac cgtgctttgt ggtagtgcga cggcggtagt
    15241 tggcccgatt gcctttatcg gcctgatgat gccgcacatg gcgcgctggc tggtaggtgc
    15301 cgatcatcgc tggtcgctgc ccgtcacgct acttgctacc cctgccctgc tgctgtttgc
    15361 cgatatcatc gggcgggtga ttgtgcccgg cgaactgcgc gtttctgtgg tcagtgcgtt
    15421 tattggtgca ccggtgctga tcttcctcgt gcgacgtaaa acgcgaggtg gcgcatgatt
    15481 tacgtctctc gccgattaat catcacctgt ttgctgttgg tttccgcctg tgtggttgca
    15541 ggtatctggg gattacgcag cggtgccgtc acgctggaaa cctcgcaggt attcgccgcg
    15601 ctgatgggcg atgcgccgcg cagtatgacg atggtggtca ccgaatggcg tttaccacgc
    15661 gtgctgatgg cgctgttgat tggcgcggca ctgggcgtca gcggcgcgat ttttcagtcg
    15721 ctgatgcgta acccgctcgg cagccctgac gtaatgggct ttaacaccgg ggcgtggagc
    15781 ggcgtgctgg tagcgatggt gctgtttggt caggacctga cggctatcgc gctggcagca
    15841 atggtgggcg gcattatcac ttcgctgttg gtctggctgc tcgcctggcg taacggcatc
    15901 gacacctttc ggttgattat tatcggtatc ggcgttcgtg ccatgctggt ggcctttaat
    15961 acctggctgt tgctgaaagc gtctttagaa acggcgctaa cagcgggttt gtggaatgca
    16021 ggatcgctca acggcctgac gtgggcaaaa acctcgcctt ccgcacccat cattatattg
    16081 atgctcattg ccgccgcctt actggtacgc cggatgcgct tgctggaaat gggcgatgat
    16141 accgcgtgtg cgctgggcgt cagcgtcgaa cgttcgcgtc tgttaatgat gctggttgca
    16201 gtggtgctta ccgctgcggc aaccgcgctt gccgggccga tttcctttat tgctttagtc
    16261 gcgccgcaca ttgcccgacg cattagcggc accgctcgct gggggctgac ccaggcggcg
    16321 ctgtgcgggg cgctgttact gctggcggcc gatctctgcg cccaacaact gtttatgccg
    16381 tatcaacttc cggttggcgt cgttaccgtc agcctcggcg gtatttacct tatcgtcttg
    16441 ttaattcagg agtctcgcaa aaaatgaacg aatctgtagc ccgtttgcgc ggcgaacagt
    16501 taaccctcgg atatggcaaa tataccgttg cggaaaatct gactgtagaa atacctgatg
    16561 gtcacttcac ggcaattatc gggccaaatg gctgcggtaa atccacttta ctgcgtactt
    16621 taagccgcct gatgacgcct gctcacgggc atgtctggct ggatggcgag cacattcaac
    16681 attacgccag taaagaggtt gcacgccgga ttggtctgtt ggcgcaaaat gctaccacgc
    16741 caggcgatat caccgtgcag gagctggtgg cgcgtgggcg ttacccgcat caaccgctat
    16801 ttacccgctg gcgcaaagaa gatgaagaag cggtaacgaa agcgatgcag gccacgggaa
    16861 taactcatct ggcagatcaa agcgtggata ccctttctgg cggacaacgc cagcgcgcgt
    16921 ggatcgcgat ggtgctggcc caggaaacgg caattatgct gctcgacgaa cccacgacct
    16981 ggctggatat cagtcatcag attgatctac tggaactgtt aagcgaactg aaccgcgaga
    17041 aaggctatac cctggcggcg gtgctgcacg atcttaatca ggcctgtcgt tacgccagcc
    17101 atttgattgc attgcgggaa gggaaaattg ttgctcaggg agcgccgaag gagattgtca
    17161 ctgctgaact gattgagcgc atttatggtc tgcgatgcat gatcattgac gatccggtag
    17221 ccggaacgcc gcttgtggtg ccgctcggac gaacggcacc ttcaaccgca aatagttaaa
    17281 ctaagtggtc tgccatcatg gcatcctgtt ttctggatgc catcgcatag cgcaataaca
    17341 cgctaccaca agccaccatc ccgccgatca gcgcggaaag gatcacaata atcgccttac
    17401 ccggaccgtc ttttttcact ggcaatgacg ggcttaactg atatttaaac ggcgtaaaat
    17461 tcacatcgtt gatatttgct tttgttaact gctcaacaag atactgacga ttacgtaatt
    17521 caccgttcag ttccgcaacg tcagtgaccg ctttttcaat ttccagtttg cgttcaatac
    17581 cgtctgcgcc gagagaaata gagaaatcgg ggtcatcttt aacggcctga ccattactgt
    17641 atacgggctt tttaattccc gccgcgttgg caatgtcgag tgaataattg aggcgctgaa
    17701 tgtttgcatc aagttgattt ttcgttttaa tgcgatcctg agccagtttt tctttttcaa
    17761 actgggtttt gatttccagt ttattacgga cgttttctag tgactctttc accaccaacg
    17821 cagagatata atcgatatac ccgctcaaaa cggtctgcgc ctcttcactg gttggcgcgg
    17881 taaaacttag cgtccaggag gtatacagtg acggttcatc ttttttctta ctggcattgt
    17941 catcaaccgc tttcattttt tcgctcaatg caacaatagc gcgatgcaaa tccagttcat
    18001 cgattttcgc ctcttttaat tggtccatca cataaggtga tgaacgcagg tactcttcca
    18061 gcaagctaac cgactgaaac ttcttgataa acaggttaaa tgcttctgta cgatcaattt
    18121 tgatatccag atccagcact cgaagcttag tgaatgtttt ctccagctct tgccactgaa
    18181 caggttctgg aggcgtgaca accgccgcgc tggtccattt ttgcggcagg atgaaagaga
    18241 tcagcaagcc tgcgcaggca aacgcaaaaa tgaccgccat gaccgttttt ttggcccgcc
    18301 ataaaactgc gattagatta agtaaatcaa tttcattatt actgggcgac gccagaggat
    18361 actcaggaaa atgagcgtca cttccctgtt taatattcag tgatgacatg gggtataacc
    18421 tgaaaatgag tccaataggg gaaatctcca cggcgctcac tttaccagtt ggtgataatt
    18481 atccgaagct gaagtttgta aattccttcc actgaat
//
LOCUS       DAZKHF020000073        18464 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2201S31241
            SAMN43267800-rid25106413.denovo.073, whole genome shotgun sequence.
ACCESSION   DAZKHF020000073 DAZKHF020000000
VERSION     DAZKHF020000073.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267800
            Sequence Read Archive: SRR30307263
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 18464)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18464)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 18464)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350581.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350581.2
            Genome Coverage       :: 151x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:21:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,652
            CDSs (total)                      :: 5,557
            Genes (coding)                    :: 5,320
            CDSs (with protein)               :: 5,320
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 237
            CDSs (without protein)            :: 237
            Pseudo Genes (ambiguous residues) :: 0 of 237
            Pseudo Genes (frameshifted)       :: 102 of 237
            Pseudo Genes (incomplete)         :: 130 of 237
            Pseudo Genes (internal stop)      :: 52 of 237
            Pseudo Genes (multiple problems)  :: 41 of 237
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18464
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267800-rid25106413.denovo.073"
                     /serotype="O 26:H11"
                     /isolate="2201S31241"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-01"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            80..730
                     /gene="csgD"
                     /locus_tag="ACHOSC_RS10360"
                     /old_locus_tag="pgaptmp_002072"
     CDS             80..730
                     /gene="csgD"
                     /locus_tag="ACHOSC_RS10360"
                     /old_locus_tag="pgaptmp_002072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biofilm master transcriptional regulator CsgD"
                     /protein_id="HGU4548012.1"
                     /translation="MFNEVHSIHGHTLLLITKPSLQATALLQHLKQSLAITGKLHNIQ
                     RSLDDISSGSIILLDMMEADKKLIHYWQDTLSRKNNNIKILLLNTPEDYPYRDIENWP
                     HINGVFYAMEDQERVVNGLQGVLRGECYFTQKLASYLITHSGNYRYNSTESALLTHRE
                     KEILNKLRIGASNNEIARSLFISENTVKTHLYNLFKKIAVKNRTQAVSWANDNLRR"
     gene            735..1124
                     /gene="csgE"
                     /locus_tag="ACHOSC_RS10365"
                     /old_locus_tag="pgaptmp_002073"
     CDS             735..1124
                     /gene="csgE"
                     /locus_tag="ACHOSC_RS10365"
                     /old_locus_tag="pgaptmp_002073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="curli production assembly/transport protein
                     CsgE"
                     /protein_id="HGU4548013.1"
                     /translation="MKRYLRWIVAAEFLFAAGNLHAVEVEVPGLLTDHTVSSIGHDFY
                     RAFSDKWESDYTGNLTINERPSARWGSWITITVNQDVIFQTFLFPLKRDFEKTVVFAL
                     IQTEEALNRRQINQALLSTGDLAHDEF"
     gene            1149..1565
                     /gene="csgF"
                     /locus_tag="ACHOSC_RS10370"
                     /old_locus_tag="pgaptmp_002074"
     CDS             1149..1565
                     /gene="csgF"
                     /locus_tag="ACHOSC_RS10370"
                     /old_locus_tag="pgaptmp_002074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="curli production assembly/transport protein
                     CsgF"
                     /protein_id="HGU4548014.1"
                     /translation="MRVKHAVVLLMLISPLSWAGTMTFQFRNPNFGGNPNNGAFLLNS
                     AQAQNSYKDPSYNDDFGIETPSALDNFTQAIQSQILGGLLSNINTGKPGRMVTNDYIV
                     DIANRDGQLQLNVTDRKTGQTSTIQVSGLQNNSTDF"
     gene            1592..2425
                     /gene="csgG"
                     /locus_tag="ACHOSC_RS10375"
                     /old_locus_tag="pgaptmp_002075"
     CDS             1592..2425
                     /gene="csgG"
                     /locus_tag="ACHOSC_RS10375"
                     /old_locus_tag="pgaptmp_002075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="curli production assembly/transport protein
                     CsgG"
                     /protein_id="HGU4548015.1"
                     /translation="MQRLFLLVAVMLLSGCLTAPPKEAARPTLMPRAQSYKDLTHLPA
                     PTGKIFVSVYNIQDETGQFKPYPASNFSTAVPQSATAMLVTALKDSRWFIPLERQGLQ
                     NLLNERKIIRAAQENGTVAINNRIPLQSLTAANIMVEGSIIGYESNVKSGGVGARYFG
                     IGADTQYQLDQIAVNLRVVNVSTGEILSSVNTSKTILSYEVQAGVFRFIDYQRLLEGE
                     VGYTSNEPVMLCLMSAIETGVIFLINDGIDRGLWDLQNKAERQNDILVKYRHMSVPPE
                     S"
     gene            complement(2489..2980)
                     /gene="ycdZ"
                     /locus_tag="ACHOSC_RS10380"
                     /old_locus_tag="pgaptmp_002076"
     CDS             complement(2489..2980)
                     /gene="ycdZ"
                     /locus_tag="ACHOSC_RS10380"
                     /old_locus_tag="pgaptmp_002076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415554.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1097 domain-containing protein"
                     /protein_id="HGU4548016.1"
                     /translation="MNILLSIAITTGILSGIWGWVAVSLGLLSWAGFLGCTAYFACPQ
                     GGLKGLAISAATLLSGVVWAMVIIYGSALAPHLEILGYVITGIVAFLMCIQAKQLLLS
                     FVPGTFIGACATFAGQGDWKLVLPSLALGLVFGYAMKNSGLWLAARSAKTAHREQEIK
                     NKA"
     gene            complement(3082..3636)
                     /gene="ycdY"
                     /locus_tag="ACHOSC_RS10385"
                     /old_locus_tag="pgaptmp_002077"
     CDS             complement(3082..3636)
                     /gene="ycdY"
                     /locus_tag="ACHOSC_RS10385"
                     /old_locus_tag="pgaptmp_002077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone YcdY"
                     /protein_id="HGU4548017.1"
                     /translation="MNEFSILCRVLGSLYYRQPQDPLLVPLFTLIREGKLAANWPLEQ
                     DELLTRLQKSCDMAQVSADYNALFIGDECAVPPYRSAWVEDATEAEVRAFLSERGMPL
                     ADTPADHIGTLLLAASWLEDQSTEDESEALETLFSEYLLPWCGAFLGKVEAHATTPFW
                     RTMAPLTRDAISAMWDELEEDSEE"
     gene            complement(3660..4397)
                     /gene="ycdX"
                     /locus_tag="ACHOSC_RS10390"
                     /old_locus_tag="pgaptmp_002078"
     CDS             complement(3660..4397)
                     /gene="ycdX"
                     /locus_tag="ACHOSC_RS10390"
                     /old_locus_tag="pgaptmp_002078"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding phosphatase"
                     /protein_id="HGU4548018.1"
                     /translation="MYPVDLHMHTVASTHAYSTLSDYIAQAKQKGIKLFAITDHGPDM
                     EDAPHHWHFINMRIWPRVVDGVGILRGIEANIKNVDGEIDCSGKMFDSLDLIIAGFHE
                     PVFAPHDKATNTQAMIATIASGNVHIISHPGNPKYEIDVKAVAEAAAKHQVALEINNS
                     SFLHSRKGSEDNCRAVAAAVRDAGGWVALGSDSHTAFTMGEFEECLKILDAVDFPPER
                     ILNVSPRRLLNFLESRGMAPIAEFADL"
     gene            complement(4452..5390)
                     /gene="ghrA"
                     /locus_tag="ACHOSC_RS10395"
                     /old_locus_tag="pgaptmp_002079"
     CDS             complement(4452..5390)
                     /gene="ghrA"
                     /locus_tag="ACHOSC_RS10395"
                     /old_locus_tag="pgaptmp_002079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415551.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016618 - hydroxypyruvate reductase
                     [NAD(P)H] activity [Evidence IEA];
                     GO_function: GO:0030267 - glyoxylate reductase (NADPH)
                     activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glyoxylate/hydroxypyruvate reductase GhrA"
                     /protein_id="HGU4548019.1"
                     /translation="MDIIFYHPTFDTQWWIEALRKAIPQARVRAWKSGDNDSADYALV
                     WHPPVEMLAGRDLKAVFALGAGVDSILSKLQAHPEMLNPSVPLFRLEDTGMGEQMQEY
                     AVSQVLHWFRRFDDYRIQQNSSHWQPLPEYHREDFTIGILGAGVLGSKVAQSLQTWRF
                     PLRCWSRTRKSWPGVQSFAGREELSAFLSQCRVLINLLPNTPETVGIINQQLLEKLPD
                     GAYLLNLARGVHVVEDDLLAALDSGKVKGAMLDVFNREPLPPESPLWQHPRVTITPHV
                     AAITRPAEAVEYISRTIAQLEKGERVCGQVDRARGY"
     gene            5624..5711
                     /locus_tag="ACHOSC_RS10400"
                     /old_locus_tag="pgaptmp_002080"
     tRNA            5624..5711
                     /locus_tag="ACHOSC_RS10400"
                     /old_locus_tag="pgaptmp_002080"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:5658..5660,aa:Ser,seq:gga)
     gene            complement(5861..6703)
                     /locus_tag="ACHOSC_RS10405"
                     /old_locus_tag="pgaptmp_002081"
     CDS             complement(5861..6703)
                     /locus_tag="ACHOSC_RS10405"
                     /old_locus_tag="pgaptmp_002081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4942 domain-containing protein"
                     /protein_id="HGU4548020.1"
                     /translation="MSDITISRPEVVNGHTDVICSTSIRHILAVRKSTLLQIDTLIRQ
                     LAEISVLTESIGGKTAPDWAMKQDFRCGCWLMEKPETAMKAITRNLDREIWRDLMQRS
                     GMLSLMDTQARDTWYRSLEYDNFPEISEANILSTFEQLHQNKDEVFERGVINVFRGLN
                     WNYKTNCPCKFGSKIIVNNLVRWDRWGFHLITGQQADRLADLERMLHLFSGKPIPDNR
                     GNITLRLDDHIQSVQGKESYEDEMFSIRYFKKGSAHITFRKPELVDRLNDIIAKHYPD
                     MLAV"
     gene            complement(6788..6985)
                     /locus_tag="ACHOSC_RS10410"
                     /old_locus_tag="pgaptmp_002082"
     CDS             complement(6788..6985)
                     /locus_tag="ACHOSC_RS10410"
                     /old_locus_tag="pgaptmp_002082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF957 domain-containing protein"
                     /protein_id="HGU4548021.1"
                     /translation="MKSLTTETALDILIAWLQDNIDCGSGIIFDNDEDKTDSAALLPC
                     IEQAREDVRTLRHLQLLHQNR"
     gene            complement(6997..7488)
                     /locus_tag="ACHOSC_RS10415"
                     /old_locus_tag="pgaptmp_002083"
     CDS             complement(6997..7488)
                     /locus_tag="ACHOSC_RS10415"
                     /old_locus_tag="pgaptmp_002083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309434.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5983 family protein"
                     /protein_id="HGU4548022.1"
                     /translation="MMKLALTLEADSVNVQALNMGRIVVDVDGIELAELINMVCDNGY
                     SLRVVDESDQTSAECTPPFATLTGIRCSTAHITETDNAWLYSLSHQTSDFGESEWIHF
                     TGSGYLLRTDAWSYPVLRLKRLGLSKTFRRLVVTLIRRYGVSLIHLDASAECLPGLPT
                     FDW"
     gene            complement(7485..7859)
                     /locus_tag="ACHOSC_RS10420"
                     /old_locus_tag="pgaptmp_002084"
     CDS             complement(7485..7859)
                     /locus_tag="ACHOSC_RS10420"
                     /old_locus_tag="pgaptmp_002084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TA system toxin CbtA family protein"
                     /protein_id="HGU4548023.1"
                     /translation="MKTLSDTHVREVSRCPSPVTIWQTLLIRLLDQHYGLTLNDTPFA
                     DERVIEQHIEAGISLCDAVNFLVEKYALVRTDQPGFSTCPRSQLINSIDILRARRATG
                     LMTRDNYRTVNNITLGKYPEAK"
     gene            complement(7906..8280)
                     /locus_tag="ACHOSC_RS10425"
                     /old_locus_tag="pgaptmp_002085"
     CDS             complement(7906..8280)
                     /locus_tag="ACHOSC_RS10425"
                     /old_locus_tag="pgaptmp_002085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV toxin-antitoxin system YeeU family
                     antitoxin"
                     /protein_id="HGU4548024.1"
                     /translation="MSDKLSGITHPDDNHDRPWWGLPCTVRPCFGARLVQEGNRLHYL
                     ADRAGIRGRFSDADAYHLDQAFPLLMKQLELMLTSGELNPRYQHTVTLNAKGLTCEAD
                     TLGSCGYVYLAVYPTPALATTS"
     gene            complement(8443..8664)
                     /locus_tag="ACHOSC_RS10430"
                     /old_locus_tag="pgaptmp_002086"
     CDS             complement(8443..8664)
                     /locus_tag="ACHOSC_RS10430"
                     /old_locus_tag="pgaptmp_002086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF987 domain-containing protein"
                     /protein_id="HGU4548025.1"
                     /translation="MKIITRGEAMRIHQQHPTSRLFPFCTGKYRWHGSAEAYTGREVQ
                     DIPGVLAVFAERRKDSFGPYVRLMSVTLN"
     gene            complement(8727..9203)
                     /locus_tag="ACHOSC_RS10435"
                     /old_locus_tag="pgaptmp_002087"
     CDS             complement(8727..9203)
                     /locus_tag="ACHOSC_RS10435"
                     /old_locus_tag="pgaptmp_002087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RadC family protein"
                     /protein_id="HGU4548026.1"
                     /translation="MQQLSFLPGEMTPGERSLILRALQTLDRHLHEPGVAFTSTRAAR
                     EWLILNMAGLEREEFRVLYLNNQNQLIAGETLFTGTINRTEVHPREVIKRALYHNAAA
                     VVLAHNHPSGEVTPSKADRLITERLVQALGLVDIRVPDHLIVGGNQVFSFAEHGLL"
     gene            complement(9219..9704)
                     /locus_tag="ACHOSC_RS10440"
                     /old_locus_tag="pgaptmp_002088"
     CDS             complement(9219..9704)
                     /locus_tag="ACHOSC_RS10440"
                     /old_locus_tag="pgaptmp_002088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antirestriction protein"
                     /protein_id="HGU4548027.1"
                     /translation="MTTVSHNSTTPSVSVTTASGNNPPQLVATPVPDEQRISFWPQHF
                     GLIPQWVTLEPRVFGWMDRLCEDYCGGIWNLYTLNNGGAFMAPEPDDDDDETWVLFNA
                     MNGNRAEMSPEAAGIAACLMTYSHHACRTECYAMTVHYYRLRDYALQHPECSAIMRII
                     D"
     gene            complement(9795..10613)
                     /locus_tag="ACHOSC_RS10445"
                     /old_locus_tag="pgaptmp_002089"
     CDS             complement(9795..10613)
                     /locus_tag="ACHOSC_RS10445"
                     /old_locus_tag="pgaptmp_002089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF932 domain-containing protein"
                     /protein_id="HGU4548028.1"
                     /translation="MRLASRFGYANQIRRDRPLTHEELMRHVPSIFGENRHTSRSEHY
                     AYIPTITVLENLQQEGFQPFFACQTRVRDQSRREYTKHMLRLRRAGQITGQHVPEIIL
                     LNSHDGSSSYQMLPGYFRAICTNGLVCGQSLGELRVPHRGNVVDRVIEGAYEVVGVFD
                     RIEEKRDAMQSLVLPPPARQALAQAALTYRYGDEHQPVTIADILTPRRREDYGKDLWS
                     AYQTIQENMLKGGISGRSAKGKRIHTRAIHSIDTDIKLNRALWVMAETLLESMR"
     gene            complement(10953..12023)
                     /locus_tag="ACHOSC_RS10450"
                     /old_locus_tag="pgaptmp_002090"
     CDS             complement(10953..12023)
                     /locus_tag="ACHOSC_RS10450"
                     /old_locus_tag="pgaptmp_002090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HGU4548029.1"
                     /translation="MSTEMKTGLVLSGGGAVGAYQAGVVKALAECGTQISMVSGTSIG
                     AFNGAIIAASPDLSEAAVRLEALWEHLGNNQVLSVNRLVYFSLLKKLFQAMNLCQIPG
                     RAGALLTTLLRHISTINGFDNLMAQPLLSDEPLTALMDHYLDTDALADGLPLYVSLYP
                     TEGGMQDIIDCIRAELGVGTTKNAVFQHIQSLPRGQQKEALLASAALPLLFRPREVQG
                     TMFGDGGMGGWRNMQGNTPVTPLVDAGCNMVIVTHLSDGSLWDRQAFPDTTILEIRPR
                     KRLKYAGDGGNSGGLLSFTSAHTDAWRQQGYEDTMLAMEHIRKPLAARQALTRSEAVL
                     QKSLDITEEADLALRNAMARIK"
     gene            complement(12020..12925)
                     /locus_tag="ACHOSC_RS10455"
                     /old_locus_tag="pgaptmp_002091"
     CDS             complement(12020..12925)
                     /locus_tag="ACHOSC_RS10455"
                     /old_locus_tag="pgaptmp_002091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase regulator RdcB family
                     protein"
                     /protein_id="HGU4548030.1"
                     /translation="MTSPFIQQIADNRVCQVLTCLPEKFVVDFANGIDVAQEHIRTAG
                     ERTFFRRLKEGLTGEGAARQNAINASLAQGVEASLRWLTEMTTSLATTNYAITRVNDR
                     VSSLVSDTARLAHYSADTREQLLTLADQVHHKLNHLEEKLHRVDQVQRAQLHLEQIFS
                     WWSAGRYASFSPAGRCYVALEELRWGAFGDVIRQGETGQVNQLLDILRHKALTQMAQE
                     SGGSATVRLNTLDWLGGQGREQADNEWHDAINWLGDWCSEEQHPVIWSTTQAAEHLPV
                     RMPRLCSAERLSESMVDEIFQKGAA"
     gene            complement(12922..15307)
                     /locus_tag="ACHOSC_RS10460"
                     /old_locus_tag="pgaptmp_002092"
                     /pseudo
     CDS             complement(12922..15307)
                     /locus_tag="ACHOSC_RS10460"
                     /old_locus_tag="pgaptmp_002092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309424.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="dynamin family protein"
     gene            complement(15418..18267)
                     /gene="cah"
                     /locus_tag="ACHOSC_RS10465"
                     /old_locus_tag="pgaptmp_002093"
     CDS             complement(15418..18267)
                     /gene="cah"
                     /locus_tag="ACHOSC_RS10465"
                     /old_locus_tag="pgaptmp_002093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="calcium-binding autotransporter Cah"
                     /protein_id="HGU4548031.1"
                     /translation="MKRHLNTSYRLVWNHITGTLVVASELARSRGKRAGVAVALSLAA
                     VTSVPALAADKVVQAGETVNDGTLTNHDNQIVFGTANGMTISTGLELGPDSEENTGGQ
                     WIQNGGIAGNTTVTTNGRQVVLEGGTASDTVIRDGGGQSLNGLAVNTTLNNRGEQWVH
                     EGGVATGTIINRDGYQSVKSGGLATGTIINTGAEGGPDSDNSYTGQKVQGTAESTTIN
                     KNGRQIILFSGLARDTLIYAGGDQSVHGRALNTTLNGGYQYVHRDGLALNTVINEGGW
                     QVVKAGGAAGNTTINQNGELRVHAGGEATAVTQNTGGALVTSTAATVIGTNRLGNFTV
                     ENGKADGVVLESGGRLDVLESHSAQNTLVDDGGTLAVSAGGKATSVTITSGGALIADS
                     GATVEGTNASGKFSIDGTSGQASGLLLENGGSFTVNAGGQAGNTTVGHRGTLTLAAGG
                     SLSGRTQLSKGASMVLNGDVVSTGDIVNAGEIRFDNQTTPNAALSRAVAKSNSPVTFH
                     KLTTTNLTGQGGTINMRVRLDGSNASDQLVINGGQATGKTWLAFTNVGNSNLGVATTG
                     QGIRVVDAQNGATTEEGAFALSRPLQAGAFNYTLNRDSDEDWYLRSENAYRAEVPLYT
                     SMLTQAMDYDRILAGSRSHQTGVNGENNSVRLSIQGGHLGHDNNGGIARGATPESSGS
                     YGFVRLEGDLLRTEVAGMSLTTGVYGAAGHSSVDVKDDDGSRAGTVRDDAGSLGGYLN
                     LVHTSSGLWADIVAQGTRHSMKASSDNNDFRARGWGWLGSLETGLPFSITDNLMLEPQ
                     LQYTWQGLSLDDGQDNAGYVKFGHGSAQHVRAGFRLGSHNDMTFGEGTSSRDTLRDSA
                     KHSVSELPVNWWVQPSVIRTFSSRGDMSMGTAAAGSNMTFSPSRNGTSLDLQAGLEAR
                     IRENITLGVQAGYAHSVSGSSAEGYNGQATLNMTF"
     gene            complement(18278..>18464)
                     /locus_tag="ACHOSC_RS10470"
                     /old_locus_tag="pgaptmp_002094"
     CDS             complement(18278..>18464)
                     /locus_tag="ACHOSC_RS10470"
                     /old_locus_tag="pgaptmp_002094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001304203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4548032.1"
                     /translation="CEKAAESLHHAERNCTQQTDTSLSDEGGERLRPGRIAVPDNSVL
                     YLPVSTFVGTGFFIHPL"
BASE COUNT         4319 a         4950 c         4602 g         4593 t
ORIGIN      
        1 catctgtcag tacttctggt gcttctattt tagaggcagc tgtcaggtgt gcgatcaata
       61 aaaaaagcgg ggtttcatca tgtttaatga agtccatagt attcatggtc atacattatt
      121 gttgatcact aaaccgtctt tgcaagcgac agctctcttg cagcacctta aacaatcgct
      181 ggcaattaca ggaaaattac ataatattca acgttctctg gacgatatat cttcaggctc
      241 tattattctt ctggatatga tggaagcaga taaaaagctt atccattatt ggcaggatac
      301 tttgagcagg aaaaacaaca atatcaaaat attgttgcta aatacgcctg aagattaccc
      361 gtaccgcgac attgaaaact ggcctcatat caacggcgtt ttttatgcca tggaggatca
      421 agaacgtgtt gtcaatgggt tgcaaggcgt cctgcgcggc gaatgctact ttacgcaaaa
      481 gcttgccagt tacctgatta cacattctgg taactatcgt tataacagca cggaatcagc
      541 cctccttact catcgggaaa aagagatcct gaataagctg cgtatcggcg cgtctaataa
      601 cgagatcgct cgttcgttgt tcatcagcga aaatacggtt aaaacgcatc tttataatct
      661 tttcaagaag atagccgtaa aaaaccggac acaagcggtt tcctgggcaa acgataacct
      721 caggcgataa agccatgaaa cgttatttac gctggattgt ggcggcagaa tttctgttcg
      781 ccgcagggaa tcttcacgcc gttgaggtag aagtcccggg attgttaact gaccatactg
      841 tttcatctat tggccatgat ttttaccgag cctttagtga taaatgggaa agtgactata
      901 cgggtaactt aacgattaat gaaaggccca gtgcacgatg gggaagctgg atcactataa
      961 cggtcaatca ggacgttatt ttccagactt ttttatttcc gttgaaaaga gacttcgaga
     1021 aaactgtcgt ctttgcactg attcaaactg aagaagcact aaatcgtcgc cagataaatc
     1081 aggcgttatt aagtacgggc gatttggcgc atgatgaatt ctaaataaaa aattgttcgg
     1141 aggctgcaat gcgtgtcaaa catgcagtag ttctactcat gcttatttcg ccattaagtt
     1201 gggctggaac catgactttc cagttccgta atccaaactt tggtggtaac ccaaataatg
     1261 gcgctttttt attaaatagc gctcaggccc aaaactctta taaagatccg agctataacg
     1321 atgactttgg tattgaaaca ccctcagcgt tagataactt tactcaggcc atccagtcac
     1381 aaattttagg tgggctactg tcgaatatta ataccggtaa accgggccgc atggtgacca
     1441 acgattatat tgttgatatt gctaaccgcg atggtcaatt gcagttgaac gtgacagatc
     1501 gtaaaaccgg acaaacctcg accatccagg tttcgggttt acaaaataac tcaaccgatt
     1561 tttaagcccc agcttcataa ggaaaataat catgcagcgc ttatttcttt tggttgccgt
     1621 catgttactg agcggatgct taaccgcccc gcctaaagaa gccgccagac cgacattaat
     1681 gcctcgagct cagagctaca aagatttgac ccatctgcca gcgccgacgg gtaagatctt
     1741 tgtttcggta tacaacattc aggacgaaac cgggcaattt aaaccctacc cggcaagtaa
     1801 cttctccact gctgttccgc aaagcgccac ggcaatgctg gtcacggcat tgaaagattc
     1861 tcgctggttt ataccgctgg agcgccaggg cttacaaaac ctgcttaacg agcgcaagat
     1921 tattcgtgcg gcacaagaaa acggcacggt tgccattaat aaccgaatcc cgctgcaatc
     1981 tttaacggcg gcaaatatta tggttgaagg ttcgattatc ggttatgaaa gcaacgttaa
     2041 atcgggcggt gttggggcaa gatattttgg gatcggtgca gacacgcaat accagctcga
     2101 tcagattgcc gtgaacctgc gcgtcgtcaa tgtgagtacc ggtgagatcc tctcctcggt
     2161 gaacaccagt aagacgatac tttcctatga agttcaggcc ggggttttcc gctttattga
     2221 ctaccagcgc ttgctggaag gggaagtggg ttacacctca aacgaacccg ttatgctgtg
     2281 tctgatgtcg gctatcgaaa caggggtcat tttcctgatt aatgatggta tcgaccgtgg
     2341 tctgtgggat ttgcagaaca aagcagaacg gcagaacgac attctggtga aataccgcca
     2401 tatgtcggtt ccaccggaat cctgacagat aaaaaagccg gaattatcat ccggcatttt
     2461 actgtttaag cgaaagcgtg agtgcccctc acgctttatt tttgatttcc tgctcacggt
     2521 gagccgtctt tgcactacgc gccgccagcc acaggccgct gtttttcatt gcgtaaccaa
     2581 ataccagccc aagcgccagc gaaggtaaca ccagtttcca gtcaccctgc ccggcaaacg
     2641 tcgcgcatgc gcctataaac gtccccggaa caaatgaaag cagcagctgt ttggcctgaa
     2701 tacacatcag aaacgcgaca ataccggtta tgacataacc gagaatttcc agatgtggtg
     2761 ccagtgcact accgtaaata atgaccatcg cccacaccac gccactaagc agggttgcag
     2821 cggagatcgc cagccctttc agcccacctt gcgggcaggc aaagtaggcc gtacagccca
     2881 ggaagcccgc ccagcttagt aagcccaggg aaacagccac ccatccccag ataccggaga
     2941 gaatgcctgt tgtgattgca atagagagaa gtatgttcat gccgcgcatg atagcagaaa
     3001 acgcggccat gaatgagatc gagcacacat tttatgcaac aacaaattat ctgttgcagt
     3061 tatgagtgac gatgatcaca tttactcttc agaatcttct tccagctcgt cccacattgc
     3121 actaatggca tcacgggtta gcggtgccat ggtgcgccag aaaggcgtgg ttgcatgggc
     3181 ctccactttg ccgaggaacg caccacacca gggtaacaga tactcactga acagtgtttc
     3241 cagtgcttcg ctctcatctt ccgttgactg atcttccagc caggaagccg caagcagcaa
     3301 tgtgccgatg tgatcggctg gcgtatccgc cagtggcatc cctcgttcgg aaagaaaagc
     3361 gcgcacttcc gcttccgttg cgtcctcaac ccatgcgcta cgatatggcg gcacggcaca
     3421 ttcatcgccg ataaacaacg cattgtaatc ggcagagact tgcgccatat cacagctttt
     3481 ctgtaaacgc gtcagtaact catcctgctc cagtggccag ttcgcagcca gtttcccctc
     3541 acgaatcagg gtaaacagcg gcaccagtaa aggatcttgc ggttggcggt aatagagcga
     3601 acccagcaca cgacagagga tagaaaactc gttcattaat atatttccat tactagtaat
     3661 taaaggtctg caaattccgc aatcggtgcc ataccgcgag attcaaggaa gttcagtaag
     3721 cggcgcggcg aaacattcaa aatgcgctct ggcggaaaat ctaccgcgtc gaggatttta
     3781 agacactctt caaattcccc catggtaaac gcggtgtgag aatccgagcc taatgccacc
     3841 caacctccag catcacgtac cgctgcggct accgcacgac agttgtcttc actgccctta
     3901 cgtgagtgta aaaatgaaga attattgatt tccagcgcca cctgatgttt cgcggctgct
     3961 tcagcaacgg ctttcacatc tatttcatat ttgggatttc cgggatggct tattatatgc
     4021 acattgccgc tggcgatagt ggcgatcatc gcttgtgtat tggtcgcttt gtcatgtggt
     4081 gcaaaaaccg gctcatgaaa accggcaata atcagatcca gcgagtcaaa cattttaccg
     4141 ctgcaatcaa tttcaccatc aacattttta atgttagctt cgatgccgcg caggatccct
     4201 accccatcaa ccactcgcgg ccagatacgc atgttaatga agtgccagtg atgcggagca
     4261 tcttccatat ccgggccatg atcggtgatc gcaaaaagtt taatgccctt ttgtttggcc
     4321 tgggcaatgt aatcactcaa tgtgctatat gcatgtgtgc tggcaacggt atgcatatga
     4381 aggtcgacgg gatacataac tctctcctgt attcaattcc cgccaaggat agcaggaatc
     4441 ctgatgcttt attagtagcc gcgtgcgcgg tcgacttgcc cgcagaccct ctcccctttt
     4501 tcgagctggg caatggtgcg agaaatgtac tccacagctt cagcgggacg ggtaatcgcg
     4561 gcgacatgtg gtgttatcgt cacgcgtgga tgttgccaga gcggactttc aggcggtaag
     4621 ggttcacgat taaaaacatc caacattgcg cctttaactt tgccgctatc cagcgccgcg
     4681 agcaggtcat cttccacaac atgaacacca cgcgccaggt tgaggagata cgcgccatcc
     4741 ggtaattttt cgagtaattg ttgattaata atgccgacgg tttcaggggt attcggtaac
     4801 aaattaatca atacccgaca ttggctcaga aatgcagaca gttcttcccg tccggcaaag
     4861 ctttgcacgc caggccacga tttacgggtt cgactccagc aacgcagcgg aaagcgccag
     4921 gtttgcagac tctgagcaac tttactgccc agtacgcctg cgcccaaaat gccgatggta
     4981 aaatcttccc gatgatattc aggcagcggt tgccaatgcg aactattttg ctggatgcga
     5041 taatcgtcaa aacgtcgaaa ccaatgcagc acctgactga cagcatattc ctgcatttgc
     5101 tcgcccatac cggtatcttc caggcgaaaa agtggaacag aagggttcag catttcaggg
     5161 tgtgcctgta gcttgctcaa aatagaatca acaccggccc cgagtgcgaa caccgcttta
     5221 agatcgcgcc ctgccagcat ttcaacagga ggatgccaga ctaaagcata atcagcagag
     5281 tcattatctc cgcttttcca cgctctgact cttgcctgag gaatagcttt gcgcagtgcc
     5341 tcaatccacc attgggtatc gaacgttggg tgataaaaga tgatatccat actgactccc
     5401 gaaaagcgtt gtgcgaaatt tattcgcact tatcgttatg atctacaaag gccaccagca
     5461 taacaaatcc gtggtcggtg gcaaaaaaag cagatttcgc ttattaaaac cacacattga
     5521 ttgaagtttg aataaacgcg cgattttttc aaaaagtttg ttgacctcag gtcatgattt
     5581 ccctaaatta gcgcccgttc cagcaagaca ggaacgacaa tttggtgagg tgtccgagtg
     5641 gctgaaggag cacgcctgga aagtgtgtat acggcaacgt atcgggggtt cgaatccccc
     5701 cctcaccgcc agattataag cgcatgttat tgtttaacat gcgctttttt attttccctt
     5761 ccagacagta tgccatcggg tatgccatct gaaaccggtt tatatgcccc gcgatcatat
     5821 cttatcggac aatttccgat ctttgagcat actggcaaag ctatactgcc agcatatcag
     5881 gatagtgttt tgcgataata tcattcagtc tgtcaaccag ttctggtttt ctgaacgtga
     5941 tatgcgcgga gcccttctta aagtatctga tgctgaacat ctcatcttca tagctctctt
     6001 taccctgaac agactgaatg tgatcatcca gacgaagggt gatgtttccc cggttgtcgg
     6061 ggattggttt accgctgaac aggtgcaaca ttctttccag atcggcgagt cggtcagcct
     6121 gttgtccggt gataagatga aatccccacc ggtcccatct caccagattg ttgacgataa
     6181 ttttactgcc aaatttacag gggcaattgg ttttgtaatt ccagttcagc cctctgaaga
     6241 cgttgatcac ccctcgctca aacacctcat ccttattctg gtgcagttgt tcaaatgtgc
     6301 tcagaatgtt cgcttcgctg atttccggaa aattatcgta ctccagtgac cggtaccatg
     6361 tatcacgggc ctgcgtatcc attaatgaca gcatcccgga acgttgcatc aggtcacgcc
     6421 agatttcgcg atcaagattc cgggtaatgg ctttcattgc tgtttcgggt ttctccatca
     6481 gccagcagcc gcagcgaaaa tcctgtttca tggcccagtc cggggcagtt ttacctccga
     6541 tgctttccgt cagcaccgag atttcagcca gttgtcggat aagtgtgtcg atttgcaaca
     6601 gtgtactctt tcgtacagcc agaatgtggc gaatgctggt tgagcagata acgtccgtat
     6661 gcccgttgac cacttccgga cgggagattg tgatatctga cataatcgat attccttttt
     6721 gcagataaat gaaaacgcca gccctgtgca ggctggcgtc agaggaatga agtctggtga
     6781 gtgagcttca ccggttctgg tgcagaagct gcagatgacg cagggtacgg acatcctccc
     6841 gggcctgttc gatacagggc aacagtgctg ctgaatccgt tttatcctca tcgttgtcaa
     6901 agataattcc cgatccgcag tcgatattgt cctgcagcca cgcaatcaga atatccagcg
     6961 ctgtttccgt ggttaatgat ttcatgttgt tcctggttac cagtcgaaag tgggtaaacc
     7021 cggcaggcat tcagcgctgg catccagatg aatgagactg acgccataac gccggatgag
     7081 tgtgacgacc agacgacgga acgtttttga cagccccagg cgtttaagcc gcagaaccgg
     7141 gtatgaccac gcatcggtac gtaacagata tccgctacct gtaaaatgaa tccattctga
     7201 ttcaccgaag tcactggtct ggtgtgacag cgagtacaac caggcgttgt ccgtttccgt
     7261 gatatgtgcg gtactgcagc gtatgccggt aagggtagca aatggtggcg tgcattctgc
     7321 tgaggtctgg tcagattcat caacaacacg aagggagtag ccgttatcgc agaccatgtt
     7381 aatcagttca gcgagctcaa taccatcgac gtcaacgaca atgcgcccca tgttcagtgc
     7441 ctgtacgtta acgctgtcgg cttccagtgt cagggccagt ttcatcattt cgcctccgga
     7501 tacttaccca aggtaatgtt atttaccgtt ctgtaattgt cgcgggtcat caggccggtc
     7561 gcccggcgag cccggaggat atcaatactg tttattaact gagagcgggg acaggtgctg
     7621 aatcccggct ggtcggtacg caccagcgcg tatttttcca cgagaaaatt aactgcatca
     7681 cacagtgaaa tgcctgcctc aatatgctgc tcaatcacac gttcatcggc aaacggtgtg
     7741 tcattcagtg tgaggccata atgctggtcc agcagtcgga tgagcagtgt ctgccagatg
     7801 gtgacgggag acgggcagcg agatacctcc cgtacatgtg tgtcggataa tgttttcatc
     7861 ttaagttctc tgtaactggt agtgctaaag gtgagagctt cggggttatg aggtggttgc
     7921 gagtgctggt gtcggataaa cagccagata aacgtaacca caggagccga gggtgtcggc
     7981 ttcgcaggtc agcccttttg cattcagcgt gacggtatgc tgatatcggg gattcagttc
     8041 accgctggtg agcatgagtt ccagttgttt catcagcagc ggaaaggcct ggtccagatg
     8101 gtatgcatct gcgtcgctga agcggcctct gataccggcg cggtcggcaa ggtaatgcag
     8161 gcggttaccc tcttgcacca gacgagcgcc aaaacagggc ctcactgtgc agggaagccc
     8221 ccaccagggg cggtcgtgat tgtcatcggg atgagttatc ccggagagtt tgtctgacac
     8281 gataaaatcc tctctgaaaa gtggttaaga atggtttaac gatgacaggt cagccggcgc
     8341 gctgaccggt ttcggtgaag atgtatgcat tgatgagaat ggcctcatcc acagcggcgt
     8401 ctgaggtcat ctggtcgtac tcgtttcccg ggtgacggaa catcagttca gggtgacact
     8461 catcagccgg acatacgggc caaaactgtc cttacggcgt tctgcaaaca cggcaagaac
     8521 gccgggaata tcctgcactt cacgaccagt atacgcttca gcactgccgt gccagcgata
     8581 cttaccggta cagaacggaa aaagacggga tgtcggatgt tgttggtgaa tacgcatggc
     8641 ttcaccacgg gtgataattt tcataatggg atacctctga agacagaaga taaaagtgaa
     8701 aacaggtgtg atgtggttgt gaagggttaa agcagaccat gttcggcaaa ggagaaaacc
     8761 tggttgccac cgactatcag atggtccggc acccggatat ccaccaggcc cagtgcctgt
     8821 accagacgtt cggtgataag ccggtctgcc ttactgggtg tgacttcacc ggacgggtga
     8881 ttgtgcgcca gcaccacggc agcggcattg tggtacaggg cgcgtttaat cacttcccgg
     8941 ggatgaactt ccgtacggtt gatggtgccg gtgaagaggg tttcaccggc aatcagctga
     9001 ttctggttgt tcagatacag cacccggaac tcttcccgct ccagtcccgc catgttcaga
     9061 atcagccatt cccgtgccgc acgggtggag gtgaaggcca cgccgggttc atgaagatgg
     9121 cggtccaggg tttgcagggc ccgcagaatg agactgcgct cgccgggcgt catctctccg
     9181 ggcagaaagg aaagctgctg cattgtccct gcctccattc agtcaatgat gcgcataatg
     9241 gcgctgcatt ccggatgctg cagggcgtaa tcccgcagcc ggtaatagtg gaccgtcatg
     9301 gcataacact ccgtacgaca ggcatgatga ctgtacgtca tcagacaggc agcaataccg
     9361 gcggcttccg ggctcatttc agcgcggttg ccgttcatgg cattgaacag tacccatgtt
     9421 tcgtcatcat cgtcatccgg ttcgggtgcc ataaatgccc cgccgttgtt cagggtgtac
     9481 agattccaga taccaccgca gtagtcttcg caaagacggt ccatccagcc gaagacacgg
     9541 ggctccaggg tcacccactg tggaatgagg ccaaaatgct gcggccagaa gctgatgcgc
     9601 tgttcatcag ggacgggagt ggcaaccagc tgaggtgggt tattccctga tgcagtggtt
     9661 acggaaacag aaggtgtggt ggaattatgc gaaacggttg tcatgaggtt attccttata
     9721 aaaagtaaat gaatggaaga agccccgggg caggaacaga tgtgagtcag gactgcactt
     9781 tcagtgaaac ggcatcagcg catgctctcc agcagcgttt ctgccatcac ccacaatgcg
     9841 cgattgagct taatgtcggt atcaatgctg tgaatggccc gggtatggat acgttttcct
     9901 tttgcactgc gaccggaaat cccgcctttc agcatattct cctggatggt ctgataagca
     9961 ctccacaggt ccttaccgta atcctcccgg cgtcgtggtg tcagaatgtc ggcgatggtg
    10021 acgggctgat gttcgtcacc ataacggtaa gtcagtgccg cctgtgccag cgcctggcgt
    10081 gccggtggcg gcaggaccag cgactgcatg gcatcacgct tctcctctat ccggtcaaaa
    10141 acgcccacca cctcgtaagc cccttcaatg actctgtcca ccacatttcc ccggtgtgga
    10201 acacgcaatt ctcccagaga ctgaccgcag accagtccat tggtacaaat ggcacgaaaa
    10261 tatccgggta acatctggta gctggatgaa ccgtcatggg agttgagcag aataatttca
    10321 gggacatgct gaccggttat ctgtccggcc cgccgcagac gcagcatgtg ttttgtgtat
    10381 tcccggcggc tctggtcgcg cacgcgggtc tggcaggcaa agaacggctg aaagccttcc
    10441 tgctgcagat tttccaggac ggtgatagtg ggaatgtacg catagtgttc actgcgagag
    10501 gtatgtcggt tttccccaaa aatactcggt acatggcgca tcagttcttc gtgtgtcagc
    10561 ggacgatcac gacgtatctg gttcgcataa ccaaaacgac tggctaatct catgatttgc
    10621 tccttatgaa tgattaagcg atataaatga aaaagccgcg tctcccggag aagacgcggc
    10681 ctgatgggtg aaatgaatga ggtttattgt ctgagaagcc cttcactggc gaaccacgta
    10741 ttaagctgta ttccggcatc gccagtgcaa ctgaccttca gcattgcgga taacccgccg
    10801 gaaatatgtt cactggttgt ttttgtatct tcccccaaaa ttaaataaat tcatcttaac
    10861 catcacccag ataatatata tctgaaaata ttttcaataa caccagacag gaaaaacacc
    10921 accacaggcg gtggcgtttc tttggggcgt ctttatttaa tccgggccat cgcgtttctc
    10981 agtgccaaat ctgcctcttc cgttatatcc aggcttttct gcaataccgc ctctgaccgg
    11041 gtcagtgcct gacgtgctgc cagcggtttc cggatatgct ccatcgccag catcgtgtct
    11101 tcatagccct gctgacgcca ggcgtcggta tgtgccgatg taaaactgag cagaccgccg
    11161 ctgttgccac catcacctgc atatttcagc cttttccggg gacggatctc aaggattgtg
    11221 gtgtccggaa aagcctggcg atcccataaa gaaccgtcac tcagatgcgt cacaatcacc
    11281 atattgcatc cggcatcgac cagaggcgtc acaggggtat ttccctgcat atttcgccat
    11341 cctcccatac caccatcacc gaacattgtc ccctgaacct cacggggacg gaacagcagg
    11401 ggcagcgcgg ctgacgcaag cagagcctct ttctgctgtc cgcggggcag gctctggata
    11461 tgctgaaaaa cggcgttttt cgtggttccg acacccagtt cagcacgaat gcagtcaata
    11521 atatcctgca tgcccccttc tgtggggtac agcgacacat acagcggtag cccgtctgcc
    11581 agagcatcag tatcaagata atgatccatc agcgctgtca ggggctcatc tgacaacaac
    11641 ggctgagcca tcagattgtc aaacccgttg attgtcgata tatggcgaag aagcgtcgta
    11701 agcagtgctc ctgcacgtcc ggggatctgg cagaggttca ttgcctggaa caattttttc
    11761 agcaatgaaa agtaaaccaa tctgtttacc gacagcacct gattattccc cagatgctcc
    11821 cagagcgcct ccaggcgtac ggcagcttct gacagatcgg gagaggccgc gataatggca
    11881 ccattgaatg cgccaatgct ggtccctgaa accatgctga tctgtgtacc acactctgcc
    11941 agtgccttaa ccactcccgc ctgataagcg cccaccgccc cgccaccgga cagcaccagc
    12001 cccgttttca tttctgtact catgcagccc ccttctgaaa tatttcatcg accatacttt
    12061 cagagaggcg ttctgcagag cagaggcgag gcatgcgaac cggtaagtgt tctgcagcct
    12121 gcgtggttga ccagatcacc ggatgctgct cttcactgca ccagtctccc agccagttaa
    12181 tcgcgtcatg ccactcgtta tctgcctgct cccggccctg accaccaagc cagtccagag
    12241 tgttcagacg cacggttgca ctaccgccac tctcctgtgc catctgcgtt aatgctttat
    12301 gtctgagaat atccagtagc tggttaacct ggcctgtttc gccctgacgt atcacatcac
    12361 caaacgcgcc ccagcgaagc tcttcaagtg ccacataaca acgtccggca ggggaaaatg
    12421 acgcatatcg cccggcgctc caccatgaga atatctgctc aagatgtaac tgcgcccgct
    12481 gaacctggtc aacacggtgg agtttttctt caagatgatt cagtttatgg tgaacctgat
    12541 cggccagggt gagtaactgc tcccgcgtat ctgcggaata atgcgccagc ctggctgtat
    12601 cactgaccag tgaactgacc ctgtcgttta cccgggtaat cgcgtagttt gtggtggcaa
    12661 gtgacgttgt catctccgtc agccagcgca gggacgcctc aacgccctgt gcaagcgacg
    12721 cattgatggc attctgccgt gcagcgcctt cgccggtcag cccctctttt aaacgccgga
    12781 agaacgtgcg ctctccggcc gtgcggatat gctcctgtgc aacatcgatt ccgtttgcga
    12841 aatcaaccac aaatttttca ggaagacagg tgagtacctg acatacccgg ttatcagcaa
    12901 tttgttgaat aaatggtgaa gtcacagcat tgtctccagc tcttccctca gcgccttact
    12961 gtcagtaatg agctcaggaa ccctttcatt taatgcctga agtcgcccgg taagccgttc
    13021 ctgcgcctgc tgatcgcgtt tatgatcttc agagctctga atcagcgtgt tacgcagttg
    13081 ttcaactttc tccctgaatg cgcagaaaaa ggcatcgatt tcctggcgga cgggctggtt
    13141 aatatcctgc tcaatggatg cctgcagctc cccgaaatat gcccgggtca gtgacataac
    13201 ttcctctctg gccttgttga tattgatcac gctgcgactc acatcctcgt catagttttc
    13261 ccagcccagg tcactggtgc caaaccatcg acaaacggtt ccccatacgc cactctgccg
    13321 acggcgacgg gtgacggtct ccgttctttc ctccataagc gcactcagat ttatttttac
    13381 cgcgagccgg gtctgtaact ggctgactgc cggaaaactg atttttacgg taaagccctc
    13441 atcctccagg cgggcattga tgcgaccggc aatgttttct gtggcgtaca gggccgtgcc
    13501 acgaaaacct gtttcaatcc cgctgacgat tttctccagc tcaggccgga actgtgcttc
    13561 agcctcacgg tgcaggctgg tcacggtcga ttcgatttgc gtcatcagtt caagggctgc
    13621 cctgcgatca ctgaatttta tttcggggct gtccggatca aagtcccgca tcctgtcatg
    13681 ctgatttccg gttccgaaaa tatcgtgaaa gaaagcgaat gcgtttctct cccggggctg
    13741 cgctgaattt tcttcctcca gcatttgctg ttctttgcgt ttaccgcttc tgaaatagtc
    13801 gttcagctcc tcctccagtg aggcacagac cttcgtcagt aattccccgg ttttggtatt
    13861 gatgtcctcc atggccattc tgacatcacc ggtcacctga ttctgacatt cctcaatctc
    13921 ctggatatcc gccagcaggc tggtaatatg cgcctgtagt gactgaatgc tttgctgcag
    13981 cccctgattg acgtaatgac agatattcgc tgacattttc tgcattctgc atcagttttg
    14041 cggcggcaga gtcaaccgcc agtgccgccg ctctggaatg ggagctctgt atgacttccg
    14101 ttatcagttg atcgatcaga gatatattcc agagtttttc tgccttttta tttaccttag
    14161 tgctgtcttt ccagtcgtcc tcttccacca taggtccgaa agcttccctg ataaaatcat
    14221 cgacccaggc ttcatcaacc ggaagcgcac cgttttttcg taactcatga agcgcacggt
    14281 tagccaggta cgcctgacgt gatgagccgg gataaacgcg ggaggcgggc agcacatcgc
    14341 tgttcagcat tgctggcacc ttctggcgta cggcatccgc accatcacca ttgcgatctt
    14401 tttcatcgaa tttattgacc agcacaaaca gacgaccggc tgagacgtca gcaatggcat
    14461 taagctcttt acgaacctcc tcatcggctt tcgagttcat ctgggtgtaa tccatcaccg
    14521 ccagcaccgc agaggctttc tgtagttgat cccgcatcat catttccatc tgtggttgtc
    14581 cggcttcatt agggccgggg gtatccagca gtgtgagtgt tccctgacat gcatccattc
    14641 ccaccaggtg gctgaacgcc acgtcgatca ccggtagttt ctgcacttct gcgtattcgt
    14701 caaagggaaa ttcagtcccc attgccgcag caagacgaac caggtcattt aacgatgcca
    14761 gtccggtaaa gatttcgtct tccccgtggt attcattttt aagccagaca tcatccgcca
    14821 gaatatccag tagttcgcgg gtatccccgg tttgctgcag ggactgtgcg acttgctgcc
    14881 ctgccggggt ggcgagtttt tcctgcagtg tgattaataa attgcggacg ggctgaatat
    14941 gttccagatg gaggaccggt tccgtttttc cgggaacgtg gcggatcaat gtcggtaccg
    15001 aggtcatggg gcggttacgg ttgggcagaa tttcctgccc cacaatggcg ttgatggtgg
    15061 ttgatttgcc cgccttcatc gtgccgacca cagccagcac catctccctg cgggcggttt
    15121 tgatttgttc gccctccagc tcctcaattc ttttcagcgc tttccgttta tcaaaaagca
    15181 gcccgttttc gttcttactg tcagataaca catccggctc atcaaccatt tgccggagca
    15241 gattgatatt cagttgcaaa agccggtcgg cttcatcaca aagcagggcg atgttctttt
    15301 cgtgcatcat tcacctttat aaattcaata attcagaact gcggtgaaca gcaatgagcg
    15361 tcctttccca ttactgcatg aattttcctg aaatcgtatt tccgggatac cgtataatca
    15421 gaaagtcata ttcagcgtag cctgaccgtt atagccttca gcgctgctgc cgctgacgct
    15481 gtgggcataa ccggcctgaa cgcccagggt gatattttcc cggatacggg cttccagtcc
    15541 ggcctgcagg tccagtgacg tgccattccg ggacggtgag aacgtcatgt tactgccggc
    15601 tgcggctgtc cccatgctca tgtcaccccg ggagctgaag gtgcggataa cagaaggctg
    15661 tacccaccag ttcaccggca gttcactcac actgtgtttt gcactgtcgc gcagggtgtc
    15721 acgggatgag gtgccttcac caaaggtcat atcgttgtgg ctgcccagac ggaaaccggc
    15781 acgcacatgt tgtgcactgc catgcccgaa cttcacataa ccggcgttat cctggccgtc
    15841 atccagggag agtccctgcc aggtgtactg cagttgtggc tccagcatca gattgtcagt
    15901 gatactgaag ggcagaccgg tttccagtga gcccagccag ccccagcccc gggcgcggaa
    15961 gtcgttattg tccgatgacg ctttcatgct gtgacgggtt ccctgggcca caatgtcagc
    16021 ccacaggccg gaggatgtgt gtaccagatt caggtatccg cccagactgc cggcatcatc
    16081 ccggaccgtg ccggcgcggg aaccgtcatc atccttaaca tcaacggaag aatggcctgc
    16141 agcaccatac acccctgtcg tcagagacat accggcaacc tctgttctga gcaggtcacc
    16201 ctccagacgg acgaagccat agctgccgct gctttccggc gtggctccac gggcaatacc
    16261 gccgttgtta tcgtgaccga gatgaccgcc ctgaatgctg agacggacgc tgttattttc
    16321 accgtttaca ccggtctgat ggctgcggga gcctgccaga atccggtcat agtccattgc
    16381 ctgtgtcaac atggatgtat acagggggac ttcagcacga taagcatttt cactgcgcag
    16441 gtaccagtct tcatcgctgt cacggttcag ggtgtagtta aaggcgccgg cctgaagcgg
    16501 gcgactcagg gcaaacgcac cttcttctgt ggtggcgcca ttctgtgcat ccacaacccg
    16561 gataccctgt ccggtggttg ccaccccgag gttgctgttt ccgacatttg taaacgcaag
    16621 ccaggttttg ccggttgcct gaccaccatt aatcaccagc tggtcagagg cattgctgcc
    16681 atcaaggcga acacgcatat tgatggtgcc gccctggccg gtgaggttcg tggtggtcag
    16741 tttatggaac gttaccgggg agttactttt tgcaacagca cggctcagcg cggcattcgg
    16801 tgtcgtctga ttatcaaagc gaatctcccc tgcgttaaca atatcgccgg tactgaccac
    16861 atcaccattc agtaccatac tggcgccttt actgagctgt gttctgccac tcagacttcc
    16921 cccggcagcc agcgtcagtg ttccacgatg tccgacagtg gtgttgccag cctgtccccc
    16981 ggcattaacc gtaaagctgc cgccattttc cagcagcagg ccgctggcct gaccggatgt
    17041 gccatcaata ctgaacttac cgctggcatt ggtcccctca acagtggcac cactgtctgc
    17101 aatcagggca ccaccggatg ttatggtgac acttgtcgcc ttaccgccgg cagacactgc
    17161 cagggtaccg ccgtcatcca ctagggtatt ctgtgctgaa tggctctcca gtacatccag
    17221 acgaccgccg gattccagaa caacaccgtc agccttaccg ttttccaccg tgaaattccc
    17281 cagacggttt gtgccgatga cagttgcagc agtactggta accagtgcac cgcccgtgtt
    17341 ctgggtgact gcagtggctt ccccgccggc atgtaccctc agttcaccgt tctgatttat
    17401 ggtggtgtta ccggcagcgc cacctgcctt aacaacctgc cagcccccct cgttaattac
    17461 cgtgttcagc gcaagtccgt ccctgtgcac atactggtaa ccgccattca gtgtggtatt
    17521 cagggccctt ccgtgtaccg actggtcacc acctgcgtaa atgagagtgt cacgggctag
    17581 cccggaaaat aagataatct gccgtccatt tttgttgatg gtggtggatt ctgctgttcc
    17641 ctggaccttc tgacccgtat acgagttgtc agaatcaggg ccgccttctg cgccggtgtt
    17701 gatgatggtt cctgttgcca gcccgccact tttaacgctc tggtaaccgt cgcggttgat
    17761 aattgtaccg gtggcaaccc cgccctcatg cacccactgc tcgcctctgt tattcagtgt
    17821 ggtgttcacc gccagtccgt tcaggctctg tcccccgccg tcacgaataa ccgtatcact
    17881 ggctgttccc ccctccagca cgacctgacg accatttgtg gtgacagtgg tgtttccggc
    17941 tatcccgcca ttctgtatcc attgcccacc ggtgttttct tcactgtccg gccccagttc
    18001 cagcccggta ctgatggtca ttccgttggc cgtaccgaag acaatctggt tgtcatgatt
    18061 tgtcagtgtt ccatcgttca cggtttctcc cgcctgtaca accttgtcag cagccagtgc
    18121 cgggactgat gtgacagcag caagagacag cgcaaccgcc acaccggcgc gttttccccg
    18181 tgagcgggcc agttcggagg ccaccaccag ggtgcccgta atgtgattcc ataccagcct
    18241 gtagctggtg ttcagatgtc gtttcatcag cttttcctta cagagggtga ataaaaaagc
    18301 cggtacccac aaaagtggat accggcaggt acaaaacaga gttatcgggc acagcgatac
    18361 gtcccgggcg taaacgctct cccccttcat cagacagaga agtatcagtc tgttgtgtgc
    18421 aattccgttc tgcatgatga agagattcag ctgccttttc gcag
//
LOCUS       DAZKHF020000074        18275 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2201S31241
            SAMN43267800-rid25106413.denovo.074, whole genome shotgun sequence.
ACCESSION   DAZKHF020000074 DAZKHF020000000
VERSION     DAZKHF020000074.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267800
            Sequence Read Archive: SRR30307263
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 18275)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18275)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 18275)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350581.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350581.2
            Genome Coverage       :: 151x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:21:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,652
            CDSs (total)                      :: 5,557
            Genes (coding)                    :: 5,320
            CDSs (with protein)               :: 5,320
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 237
            CDSs (without protein)            :: 237
            Pseudo Genes (ambiguous residues) :: 0 of 237
            Pseudo Genes (frameshifted)       :: 102 of 237
            Pseudo Genes (incomplete)         :: 130 of 237
            Pseudo Genes (internal stop)      :: 52 of 237
            Pseudo Genes (multiple problems)  :: 41 of 237
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18275
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267800-rid25106413.denovo.074"
                     /serotype="O 26:H11"
                     /isolate="2201S31241"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-01"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            51..776
                     /locus_tag="ACHOSC_RS10475"
                     /old_locus_tag="pgaptmp_002095"
     CDS             51..776
                     /locus_tag="ACHOSC_RS10475"
                     /old_locus_tag="pgaptmp_002095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HGU4548033.1"
                     /translation="MLPGCCKNGIVISKIPVMQAGLKEVMRTHFPEYEIISSASAEDL
                     TLLQLRRSGLVIADLAGESEDPRSVCEHYYSLISQYREIHWVFMVSRSWYSQAVELLM
                     CPTATLLSDVEPIENLVKTVRSGNTHAERISAMLTSPAMTETHDFSYRSVILTLSERK
                     VLRLLGKGWGINQIASLLKKSNKTISAQKNSAMRRLAIHSNAEMYAWINSAQGARELN
                     LPSVYGDAAEWNTAELRREMSHS"
     gene            734..1411
                     /gene="bglJ"
                     /locus_tag="ACHOSC_RS10480"
                     /old_locus_tag="pgaptmp_002096"
     CDS             734..1411
                     /gene="bglJ"
                     /locus_tag="ACHOSC_RS10480"
                     /old_locus_tag="pgaptmp_002096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418786.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional activator BglJ"
                     /protein_id="HGU4548034.1"
                     /translation="MEHSRIKKRNVALIEKCVMSSIGIESLFRKFAGNPYKLHTYTSQ
                     ESFQDAMTRISFAAVIFSFSAMRSERREGLSCLTELAIKLPRTRRLVIADDDIEARLI
                     GSLSPSPLDGVLSKASTLEIFHQELFLSLNGVRQATDRLNNQWYINQSRTLSPTEREI
                     LRFMSRGYSMTQIAEQLKRNIKTIRAHKFNVMSKLGVSSDAGLLEAADILLCMRHCEA
                     SNVLHPY"
     gene            complement(1449..2237)
                     /gene="fhuF"
                     /locus_tag="ACHOSC_RS10485"
                     /old_locus_tag="pgaptmp_002097"
     CDS             complement(1449..2237)
                     /gene="fhuF"
                     /locus_tag="ACHOSC_RS10485"
                     /old_locus_tag="pgaptmp_002097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="siderophore-iron reductase FhuF"
                     /protein_id="HGU4548035.1"
                     /translation="MAYRSAPLYEDVIWRTHLQPQDAGLAQAVRATIAEHREHLLEFI
                     RLDEPAPLRAMTLAQWSSPNALSSLLAVYSDHIYRNQPTMIRENKPLISLWAQWYIGL
                     MVPPLMLALLTQEKALDVSPEHFHVEFHETGRAACFWVDVCEDKNATPHSPQQRMETL
                     ISQALVPVVQALEATGEINGKLIWSNTGYLINWYLTEMKQLLGEATVESLRHALFFEK
                     TLTTGEDNPLWRTVVLRDGLLVRRTCCQRYRLPDVQQCGDCTLK"
     gene            2378..2614
                     /locus_tag="ACHOSC_RS10490"
                     /old_locus_tag="pgaptmp_002098"
     CDS             2378..2614
                     /locus_tag="ACHOSC_RS10490"
                     /old_locus_tag="pgaptmp_002098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313355.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1435 domain-containing protein"
                     /protein_id="HGU4548036.1"
                     /translation="MLQRTLGSGWGVLLPGLLIAGLMYADLSPDQWRIVILMGLILTP
                     VMLYHKQLRHYVLLPSCLALIAGIMLMLMNLNQG"
     gene            complement(2653..2739)
                     /locus_tag="ACHOSC_RS10495"
                     /old_locus_tag="pgaptmp_002099"
     tRNA            complement(2653..2739)
                     /locus_tag="ACHOSC_RS10495"
                     /old_locus_tag="pgaptmp_002099"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2703..2705),aa:Leu,seq:cag)
     gene            complement(2768..2854)
                     /locus_tag="ACHOSC_RS10500"
                     /old_locus_tag="pgaptmp_002100"
     tRNA            complement(2768..2854)
                     /locus_tag="ACHOSC_RS10500"
                     /old_locus_tag="pgaptmp_002100"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2818..2820),aa:Leu,seq:cag)
     gene            complement(3155..4186)
                     /gene="rsmC"
                     /locus_tag="ACHOSC_RS10505"
                     /old_locus_tag="pgaptmp_002101"
     CDS             complement(3155..4186)
                     /gene="rsmC"
                     /locus_tag="ACHOSC_RS10505"
                     /old_locus_tag="pgaptmp_002101"
                     /EC_number="2.1.1.172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0008990 - rRNA
                     (guanine-N2-)-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(1207)-N(2))-methyltransferase
                     RsmC"
                     /protein_id="HGU4548037.1"
                     /translation="MSAFTPASEVLLRHSDDFEQSRILFAGDLQDDLPARLDTAASRA
                     HTQQFHHWQVLSRQMGDNARFSLVAKADDVADCDTLIYYWPKNKPEAQFQLMNLLSLL
                     PVGTDIFVVGENRSGVRSAEQMLADYAPLNKVDSARRCGLYFGRLEKQPVFDADKFWG
                     EYSVDGLTVKTLPGVFSRDGLDVGSQLLLSTLTPHTKGKVLDVGCGAGVLSVAFARHS
                     PKIRLTLCDVSAPAVEASRATLAANGVEGEVFASNVFSEVKGRFDMIISNPPFHDGMQ
                     TSLDAAQTLIRGAVRHLNSGGELRIVANAFLPYPDVLDETFGFHEVIAQTGRFKVYRA
                     IMTRQAKKG"
     gene            4289..4702
                     /gene="holD"
                     /locus_tag="ACHOSC_RS10510"
                     /old_locus_tag="pgaptmp_002102"
     CDS             4289..4702
                     /gene="holD"
                     /locus_tag="ACHOSC_RS10510"
                     /old_locus_tag="pgaptmp_002102"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit psi"
                     /protein_id="HGU4548038.1"
                     /translation="MTSRRDWQLQQLGITQWSLRRPGALQGEIAIAIPAHVRLVMVAN
                     DLPALTDPLVSDVLRALTVSPDQVLQLTPEKIAMLPQGSRCNSWRLGTDEPLSLEGAQ
                     VASPALTDLRANPTARAALWQQICTYEHDFFPRND"
     gene            4671..5117
                     /gene="rimI"
                     /locus_tag="ACHOSC_RS10515"
                     /old_locus_tag="pgaptmp_002103"
     CDS             4671..5117
                     /gene="rimI"
                     /locus_tag="ACHOSC_RS10515"
                     /old_locus_tag="pgaptmp_002103"
                     /EC_number="2.3.1.266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006474 - N-terminal protein amino acid
                     acetylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal protein S18-alanine
                     N-acetyltransferase"
                     /protein_id="HGU4548039.1"
                     /translation="MNTISSLETTDLPAAYHIEQRAHAFPWSEKTFASNQGERYLNFQ
                     LTQNGKMAAFAITQVVLDEATLFNIAVDPDYQRQGLGRALLEHLIDELEKRGVATLWL
                     EVRASNAAAIALYESLGFNEATIRRNYYPTTDGREDAIIMALPISM"
     gene            5132..5809
                     /gene="yjjG"
                     /locus_tag="ACHOSC_RS10520"
                     /old_locus_tag="pgaptmp_002104"
     CDS             5132..5809
                     /gene="yjjG"
                     /locus_tag="ACHOSC_RS10520"
                     /old_locus_tag="pgaptmp_002104"
                     /EC_number="3.1.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008253 - 5'-nucleotidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine 5'-nucleotidase"
                     /protein_id="HGU4548040.1"
                     /translation="MKWDWIFFDADETLFTFDSFTGLQRMFLDYSVTFTAEDFQDYQA
                     VNKPLWVDYQNGAITSLQLQHGRFESWAERLKVEAGLLNDAFINAMAEICTPLPGAVS
                     LLNAIRGNAKIGIITNGFSALQQVRLERTGLRDYFDLLVISEEVGVAKPNKKIFDYAL
                     EQAGNPDRSRVLMVGDTAESDILGGINAGLATCWLNAHNREQPEGIAPTWTVSSLHEL
                     EQLLCKH"
     gene            5900..7489
                     /gene="prfC"
                     /locus_tag="ACHOSC_RS10525"
                     /old_locus_tag="pgaptmp_002105"
     CDS             5900..7489
                     /gene="prfC"
                     /locus_tag="ACHOSC_RS10525"
                     /old_locus_tag="pgaptmp_002105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003747 - translation release factor
                     activity [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 3"
                     /protein_id="HGU4548041.1"
                     /translation="MTLSPYLQEVAKRRTFAIISHPDAGKTTITEKVLLFGQAIQTAG
                     TVKGRGSNQHAKSDWMEMEKQRGISITTSVMQFPYHDCLVNLLDTPGHEDFSEDTYRT
                     LTAVDCCLMVIDAAKGVEDRTRKLMEVTRLRDTPILTFMNKLDRDIRDPMELLDEVEN
                     ELKIGCAPITWPIGCGKLFKGVYHLYKDETYLYQSGKGHTIQEVRIVKGLNNPDLDAA
                     VGEDLAQQLRDELELVKGASNEFDKELFLAGEITPVFFGTALGNFGVDHMLDGLVEWA
                     PAPMPRQTDTRTVEASEDKFTGFVFKIQANMDPKHRDRVAFMRVVSGKYEKGMKLRQV
                     RTAKDVVISDALTFMAGDRSHVEEAYPGDILGLHNHGTIQIGDTFTQGEMMKFTGIPN
                     FAPELFRRIRLKDPLKQKQLLKGLVQLSEEGAVQVFRPISNNDLIVGAVGVLQFDVVV
                     SRLKSEYNVEAVYESVNVATARWVECADAKKFEEFKRKNESQLALDGGDNLAYIATSM
                     VNLRLAQERYPDVQFHQTREH"
     gene            7882..8487
                     /gene="osmY"
                     /locus_tag="ACHOSC_RS10530"
                     /old_locus_tag="pgaptmp_002106"
     CDS             7882..8487
                     /gene="osmY"
                     /locus_tag="ACHOSC_RS10530"
                     /old_locus_tag="pgaptmp_002106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone OsmY"
                     /protein_id="HGU4548042.1"
                     /translation="MTMTRLKISKTLLAVMLTSAVATGSAYAENNAQTTNESAGQKVD
                     SSMNKVGNFMDDSAITAKVKAALVDHDNIKSTDISVKTDQKVVTLSGFVESQAQAEEA
                     VKVAKGVEGVTSVSDKLHVRDAKEGSVKGYAGDTATTSEIKAKLLADDIVPSRHVKVE
                     TTDGVVQLSGTVDSQAQSDRAESIAKAVDGVKSVKNDLKTK"
     gene            8614..8775
                     /locus_tag="ACHOSC_RS10535"
                     /old_locus_tag="pgaptmp_002107"
     CDS             8614..8775
                     /locus_tag="ACHOSC_RS10535"
                     /old_locus_tag="pgaptmp_002107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002887716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1328 domain-containing protein"
                     /protein_id="HGU4548043.1"
                     /translation="MFRWGIIFLVIALIAAALGFGGLAGTAAGAAKIVFVVGIILFLV
                     SLFMGRKRP"
     gene            8897..9970
                     /locus_tag="ACHOSC_RS10540"
                     /old_locus_tag="pgaptmp_002108"
     CDS             8897..9970
                     /locus_tag="ACHOSC_RS10540"
                     /old_locus_tag="pgaptmp_002108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="patatin family protein"
                     /protein_id="HGU4548044.1"
                     /translation="MGQRIPVTLGNIAPLSLRPFQPGRIALVCEGGGQRGIFTAGVLD
                     EFMRAQFNPFDLYLGTSAGAQNLSAFICNQPGYARKVIMRYTTKREFFDPLRFVRGGN
                     LIDLDWLVEATASQMPLQMDTAARLFDSGKSFYMCACRQDDYAPNYFLPTKQNWLDVI
                     RASSAIPGFYRSGVSLEGINYLDGGISDAIPVKEAARQGAKTLVVIRTVPSQMYYTPQ
                     WFKRMERWLGDSSLQPLVNLVQHHETSYRDIQQFIEKPPGKLRIFEIYPPKPLHSIAL
                     GSRIPALREDYKLGRLCGRYFLATVGKLLTEKAPLTRHLVPVVTPESIVIPPAPVAND
                     TLVAEVSDAPQANDPTFNNEDLA"
     gene            9967..10749
                     /locus_tag="ACHOSC_RS10545"
                     /old_locus_tag="pgaptmp_002109"
     CDS             9967..10749
                     /locus_tag="ACHOSC_RS10545"
                     /old_locus_tag="pgaptmp_002109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313363.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HGU4548045.1"
                     /translation="MICRFIDTHCHFDFPPFSGDEEASLQRAAQAGVGKIIVPATEAE
                     NFARVQALAEKYQPLYAALGLHPGMLEKHSDVSLDQLQQALERRPAKVVAVGEIGLDL
                     FGDDPQFERQQWLLDEQLKLAKRYDLPVILHSRRTHDKLAMHLKRHDLPCTGVVHGFS
                     GSLQQAERFVQLGYKIGVGGTITYPRASKTRDVIAKLPLASLLLETDAPDMPLNGFQG
                     QPNRPEQAVRVFDVLCELRPEPEDEIAEVLLNNTYALFSVSG"
     gene            complement(11266..12129)
                     /locus_tag="ACHOSC_RS10550"
                     /old_locus_tag="pgaptmp_002110"
     CDS             complement(11266..12129)
                     /locus_tag="ACHOSC_RS10550"
                     /old_locus_tag="pgaptmp_002110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YjjW family glycine radical enzyme activase"
                     /protein_id="HGU4548046.1"
                     /translation="MNSRCALVSKIIPFSCVDGPGSRLALFLQGCNLRCKNCHNPWTM
                     GRCNDCGECVPQCPHQALQIVDGKVLWNAVVCEQCDTCLKMCPQHATPMAQSMSVDEV
                     LSHIRKAVLFIEGITVSGGEATTQLPFVVALFTAIKNDPQLRHLTCLVDSNGMLSETG
                     WEKLLPVCDGAMLDLKAWGSECHQQLTGRDNQQIKRSICLLAERGKLAELRLLVIPGQ
                     VDYLQHIEELAVFIKGLGDVPVRLNAFHAHGVYGEAQSWASATPEDVEPLADALKVRG
                     VSRLIFPALYL"
     gene            complement(12101..13651)
                     /locus_tag="ACHOSC_RS10555"
                     /old_locus_tag="pgaptmp_002111"
     CDS             complement(12101..13651)
                     /locus_tag="ACHOSC_RS10555"
                     /old_locus_tag="pgaptmp_002111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YjjI family glycine radical enzyme"
                     /protein_id="HGU4548047.1"
                     /translation="MPTSHENALQQRCQQIVTSPVLSPEQKRHFLALEAENNLPYPQL
                     PAEARRALDEGVICDMFEGHAPYKPRYVLPDYARFLANGSEWLELEGAKDLDDALSLL
                     TILYHHVPSVTSMPVYLGQLDALLQPYVRILTQDEIDIRIKRFWRYLDRTLPDAFMHA
                     NIGPSDSPITRAILRADAELKQVSPNLTFIYDPEITPDDLLLEVAKNICECSKPHIAN
                     GPVHDKIFTKGGYGIVSCYNSLPLAGGGSTLVRLNLKAIAERSESLDDFFTRTLPHYC
                     QQQIAIIDARCEFLYQQSHFFENSFLVKEGLINPERFVPMFGMYGLAEAVNLLCEKEG
                     IAARYGKEAAANEVGYRISAQLAEFVANTPVKYGWQKRAMLHAQSGISSDIGTTPGAR
                     LPYGDEPDPITHLQTVAPHHAYYYSGISDILTLDETIKRNPQALVQLCLGAFKAGMRE
                     FTANVSGNDLVRVTGYMVRLSDLEKYRAEGSRTNTTWLGEEAARNTRILERQPRVISH
                     EQQMRFSQ"
     gene            13909..14688
                     /gene="deoC"
                     /locus_tag="ACHOSC_RS10560"
                     /old_locus_tag="pgaptmp_002112"
     CDS             13909..14688
                     /gene="deoC"
                     /locus_tag="ACHOSC_RS10560"
                     /old_locus_tag="pgaptmp_002112"
                     /EC_number="4.1.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005097947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004139 - deoxyribose-phosphate aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0009264 - deoxyribonucleotide catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribose-phosphate aldolase"
                     /protein_id="HGU4548048.1"
                     /translation="MTDLKASSLRALKLMDLTTLNDDDTDEKVIALCHQAKTPVGNTA
                     AICIYPRFIPIARKTLKEQGTPEIRIATVTNFPHGNDDIEIALAETRAAIAYGADEVD
                     VVFPYRALMAGNEQVGFDLVKACKEACAAANVLLKVIIETGELKDEALIRKASEISIK
                     AGADFIKTSTGKVAVNATPESARIMMEVIRDMGVEKTVGFKPAGGVRTAEDAQKYLAI
                     ADELFGADWADARHYRFGASSLLASLLKALGHGDGKSASSY"
     gene            14815..16137
                     /gene="deoA"
                     /locus_tag="ACHOSC_RS10565"
                     /old_locus_tag="pgaptmp_002113"
     CDS             14815..16137
                     /gene="deoA"
                     /locus_tag="ACHOSC_RS10565"
                     /old_locus_tag="pgaptmp_002113"
                     /EC_number="2.4.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009032 - thymidine phosphorylase activity
                     [Evidence IEA];
                     GO_process: GO:0006213 - pyrimidine nucleoside metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thymidine phosphorylase"
                     /protein_id="HGU4548049.1"
                     /translation="MFLAQEIIRKKRDGHALSDEEIRFFINGIRDNTISEGQIAALAM
                     TIFFHDMTMPERVSLTMAMRDSGTVLDWKSLHLNGPIVDKHSTGGVGDVTSLMLGPMV
                     AACGGYIPMISGRGLGHTGGTLDKLESIPGFDIFPDDNRFREIIKDVGVAIIGQTSSL
                     APADKRFYATRDITATVDSIPLITASILAKKLAEGLDALVMDVKVGSGAFMPTYELSE
                     ALAEAIVGVANGAGVRTTALLTDMNQVLASSAGNAVEVREAVQFLTGEYRNPRLFDVT
                     MALCVEMLISGKLAKDDAEARAKLQAVLDNGKAAEVFGRMVAAQKGPTDFVENYAKYL
                     PTAMLTKAVYADTEGFVSEMDTRALGMAVVAMGGGRRQASDTIDYSVGFTDMARLGDQ
                     VDGQRPLAVIHAKDENSWQEAAKAVKAAIKLADKAPESTPTVYRRISE"
     gene            16189..17412
                     /gene="deoB"
                     /locus_tag="ACHOSC_RS10570"
                     /old_locus_tag="pgaptmp_002114"
     CDS             16189..17412
                     /gene="deoB"
                     /locus_tag="ACHOSC_RS10570"
                     /old_locus_tag="pgaptmp_002114"
                     /EC_number="5.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008973 - phosphopentomutase activity
                     [Evidence IEA];
                     GO_process: GO:0009117 - nucleotide metabolic process
                     [Evidence IEA];
                     GO_process: GO:0043094 - cellular metabolic compound
                     salvage [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopentomutase"
                     /protein_id="HGU4548050.1"
                     /translation="MKRAFIMVLDSFGIGATEDAERFGDVGADTLGHIAEACAKGEAD
                     NGRKGPLNLPNLTRLGLAKAHEGSTGFIPAGMDGNAEVIGAYAWAHEMSSGKDTPSGH
                     WEIAGVPVLFEWGYFSDHENSFPQELLDKLVERANLPGYLGNCHSSGTVILDQLGEEH
                     MKTGKPIFYTSADSVFQIACHEETFGLDKLYELCEIAREELTNGGYNIGRVIARPFIG
                     DKAGNFQRTGNRHDLAVEPPAPTVLQKLVDEKHGQVVSVGKIADIYANCGITKKVKAT
                     GLDALFDATIKEMKEAGDNTIVFTNFVDFDSSWGHRRDVAGYAAGLELFDRRLPELMS
                     LLRDDDILILTADHGCDPTWTGTDHTREHIPVLVYGPKVKPGSLGHRETFADIGQTLA
                     KYFGTSDMEYGKAMF"
     gene            17492..18211
                     /gene="deoD"
                     /locus_tag="ACHOSC_RS10575"
                     /old_locus_tag="pgaptmp_002115"
     CDS             17492..18211
                     /gene="deoD"
                     /locus_tag="ACHOSC_RS10575"
                     /old_locus_tag="pgaptmp_002115"
                     /EC_number="2.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710124.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004731 - purine-nucleoside phosphorylase
                     activity [Evidence IEA];
                     GO_process: GO:0006139 - nucleobase-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="purine-nucleoside phosphorylase"
                     /protein_id="HGU4548051.1"
                     /translation="MATPHINAEMGDFADVVLMPGDPLRAKYIAETFLEDAREVNNVR
                     GMLGFTGTYKGRKISVMGHGMGIPSCSIYTKELITDFGVKKIIRVGSCGAVLPHVKLR
                     DVVIGMGACTDSKVNRIRFKDHDFAAIADFDMVRNAVDAAKALGVDARVGNLFSADLF
                     YSPDGEMFDVMEKYGILGVEMEAAGIYGVAAEFGAKALTICTVSDHIRTHEQTTAAER
                     QTTFNDMIKIALESVLLGDKE"
BASE COUNT         4367 a         4695 c         4935 g         4278 t
ORIGIN      
        1 atatcctgaa taaaccatca taatttttag acagtggatg tggagggaat atgttgccag
       61 gatgctgcaa aaatggaatt gttatcagta aaatacctgt tatgcaagca gggttaaaag
      121 aggtcatgag gactcacttc cctgaatatg aaataatatc cagcgcctct gcggaggacc
      181 ttaccttatt acaattacgc cgttccggat tagtcattgc tgatttagcc ggtgaaagtg
      241 aagatccacg ttctgtttgt gaacattatt attctttaat ctcacaatat cgggaaattc
      301 actgggtttt catggtgtca cgctcctggt attcccaggc agtagaactg ctcatgtgcc
      361 ctacggcgac gttattgtct gatgttgaac ccattgagaa tctggtcaag accgtacgtt
      421 ccggcaatac gcacgcagag cgtatcagcg ccatgctgac ctctccggca atgactgaaa
      481 ctcatgattt tagctatcgc tccgtcattc tcactctttc agagcgcaag gtactgcggc
      541 tattaggtaa aggatggggc atcaaccaga tagcttcatt gcttaagaaa agtaataaaa
      601 ctatcagcgc ccaaaaaaac agtgcgatgc gtcgactggc aattcacagc aacgctgaaa
      661 tgtatgcatg gataaatagc gcgcagggtg caagagaact taacttgcct tctgtttatg
      721 gagatgccgc agaatggaac acagccgaat taagaagaga aatgtcgcac tcatagaaaa
      781 atgcgtcatg agtagtatcg gtattgagag tttattcaga aagtttgcgg gtaaccctta
      841 taagctccat acctatacca gtcaggagtc atttcaggat gccatgacgc ggatctcgtt
      901 tgcggcggtc attttttctt tttctgccat gagaagtgag cgcagagagg gattatcttg
      961 cctgactgaa ctggcgatta agcttccgcg tacccggcgt ttagttattg cggatgatga
     1021 tattgaagct cggctgattg gttcattgtc gccatcaccg ctggacggtg tattaagtaa
     1081 agcgtcaacg ctggagattt ttcatcagga actctttttg tcattaaatg gtgtacgtca
     1141 ggcgaccgac cgactgaaca atcagtggta cattaaccaa agccggacgc taagcccgac
     1201 ggagagagaa atattgcgct ttatgtcgcg tggttactca atgacacaaa ttgccgagca
     1261 gcttaaacgt aatatcaaaa cgatccgtgc acataaattt aatgtgatgt cgaaactggg
     1321 cgtcagttct gatgcagggt tgttggaggc cgcagatatt ctgttatgta tgcggcattg
     1381 cgaagcaagt aatgtgttgc atccctatta atccgcatga tgccgggttt acttccccgg
     1441 cagtgctttc atttcagcgt acaatcgcca cattgctgca catccggtaa gcgataacgc
     1501 tggcagcaag tgcggcgcac cagcaggccg tcgcgcagta ccacggtacg ccagagtgga
     1561 ttatcttcac cggtagtaag cgttttctca aaaaagaggg catggcgcag cgattcaacc
     1621 gtcgcctcgc cgagcagctg tttcatctca gtcagatacc agttgatcaa ataaccggta
     1681 ttactccaga taagtttgcc gttgatctct cccgtcgctt ctagtgcctg cacaaccgga
     1741 accagcgcct ggctgattaa cgtttccatt cgctgctgcg gcgaatgtgg tgttgcgttt
     1801 ttatcttcac acacatcgac ccagaaacag gcggcacggc cggtttcgtg aaactcaacg
     1861 tggaaatgtt ccggcgacac atctaacgct ttttcctgcg tcagcagcgc cagcattaat
     1921 ggtggcacca tcaggccgat ataccattgc gcccatagtg agatcagcgg cttgttctcg
     1981 cggatcatcg ttggttggtt gcgatagata tgatcggaat agaccgccag tagagaactt
     2041 agcgcattcg gtgatgacca ttgcgccagc gtcatggcgc gaagcggggc gggttcatcc
     2101 agacggataa actccagcaa atgttcacga tgttcggcga tcgtcgcccg tacggcttgt
     2161 gcaagcccag catcctgcgg ctggagatgc gttcgccaga tgacatcttc atagagcggt
     2221 gcggaacgat aggccataat cgggatagta atctaaatga taatgattgc taatcctagc
     2281 gataggttta cccgatagca agggatttct ctggcttgca aatgataaaa attatcatat
     2341 gatattggtt atcattatca atgaaagaga tgaaaccatg ttgcaacgta cgctgggcag
     2401 tggctgggga gtgttgctgc cgggattgct gattgcaggg ctgatgtatg cggatttatc
     2461 gccggatcaa tggcggattg tcattctgat gggattgata ttgacgccgg tgatgctgta
     2521 tcacaaacag ttacggcatt acgttttgct accatcgtgc ctggcgctta ttgctggcat
     2581 catgctgatg ctaatgaatt tgaatcaggg atgaaaaatc aaggaagaaa caagaaagga
     2641 agtaaagata attggtgcga ggggggggac ttgaaccccc acgtccgtaa gaacactaac
     2701 acctgaagct agcgcgtcta ccaattccgc caccttcgcc cagtgcgagc aatatcaacg
     2761 tggtttttgg tgcgaggggg gggacttgaa cccccacgtc cgtaaggaca ctaacacctg
     2821 aagctagcgc gtctaccaat tccgccacct tcgcatacca tcaattctta aaaagaattg
     2881 ctaccacgga ggcgcattct agtggttttc agcttttcgt caatagttaa ttatcgccgg
     2941 aggtgtaatt gctggaaaaa tgtccatcag gaaactaacg tgcaggtttt gtaggtcgga
     3001 taaggcgttc acgccgcatc cgacacggta tttgggtgca gatgccagat gcgacgcggg
     3061 cgcgtcttat ctggcctacg aagggctaac gtgcaggttt tgtaggttgt ataaggcgag
     3121 cacgccgcat ccgacaaggc ttgcggcggg atacttaacc tttcttcgcc tggcgggtca
     3181 taatggcgcg atacaccttg aagcgcccgg tttgcgcgat aacttcatgg aagccaaatg
     3241 tctcatccag cacgtccggg taaggcagga aggcgttcgc tacaattcgc agctcgccgc
     3301 cgctattaag atgacgcacc gcgccgcgaa tcagcgtttg cgccgcatcc aggctggttt
     3361 gcatcccatc gtggaacggc gggttggaga tgatcatatc aaaacgacct ttcacctcgg
     3421 aaaagacgtt gctggcaaag acttcacctt caacaccgtt ggccgcaagc gtcgcacggc
     3481 tggcttctac cgccggggca gagacatcgc acaaggtgag acgaattttc ggcgaatggc
     3541 gcgcaaaggc aactgaaagc acacccgcgc cacagccgac atccagcact ttacctttcg
     3601 tgtgcggggt taacgtcgag agcagcaact ggctaccgac atccagaccg tcgcggctaa
     3661 acacgccagg cagcgttttg accgtcaggc catcgacgct gtattcgccc cagaatttat
     3721 ccgcatcaaa tactggctgt ttttccagac gaccaaaata gaggccacag cgacgagcgc
     3781 tgtcgacttt attcaacggc gcataatctg ccagcatctg ctcggcgctg cgcacgccgc
     3841 tgcggttctc gccaacgaca aaaatatccg tccccaccgg cagcagagaa agtaaattca
     3901 tcaactggaa ctgggcttcc ggtttgttct tcggccagta gtaaatcagc gtatcgcaat
     3961 ctgcgacgtc atccgccttg gcgaccagac taaaacgggc gttatccccc atctggcggc
     4021 ttaatacctg ccagtggtgg aattgctggg tatgagcacg gctggccgcg gtatctaaac
     4081 gcgcgggcag gtcatcctgt aagtctccgg caaacagaat acggctttgt tcgaaatcat
     4141 cactgtgacg cagcaagact tcacttgccg gggtaaatgc agacatggaa tgctcctcaa
     4201 ttgatactgg cggcgattat agccatatgt tggcgcggta tcgacgaatt tgctatattt
     4261 gcgcccctga caacaggagc gattcgctat gacatcccga cgagactggc agttacagca
     4321 actgggcatt acccagtggt cgctgcgtcg ccctggcgcg ttgcaggggg aaattgccat
     4381 tgcgatcccg gcacacgtcc gtctggtgat ggtggcaaac gatcttcccg ccctgactga
     4441 tcctttagtg agcgatgttc tgcgcgcatt aaccgtcagc cccgatcaag tgctgcaact
     4501 gacgccagaa aaaatcgcga tgctgccgca aggcagtcgc tgcaacagct ggcggttggg
     4561 tactgacgaa ccgctatcac tggaaggcgc tcaggtggca tcaccggcgc tcaccgattt
     4621 acgggcaaac ccaacggcac gcgccgcgtt atggcaacaa atttgcacat atgaacacga
     4681 tttcttccct cgaaacgact gatttaccag cggcttacca cattgaacaa cgcgcccacg
     4741 cctttccgtg gagtgaaaaa acctttgcca gcaaccaggg cgagcgttat ctcaactttc
     4801 agttaacgca aaacggcaaa atggcggcgt ttgcgattac gcaagtggtg ctggatgaag
     4861 ctacattgtt caatattgcg gtcgatcctg actatcagcg tcagggattg ggaagggcgc
     4921 tgctggaaca tctgatcgac gaactggaaa aacgcggcgt ggcgacgctc tggctggaag
     4981 tccgtgcctc aaatgctgcc gccattgccc tgtacgaaag tttaggcttt aacgaggcga
     5041 cgattcgccg caattactac cccaccacgg acggtcgcga agacgccatc atcatggcgt
     5101 tgccaatcag tatgtaagac aaggtggaat aatgaagtgg gactggattt tctttgatgc
     5161 cgatgaaacg ctgtttacct ttgactcatt caccggcctg cagcggatgt ttcttgatta
     5221 cagcgtcacc tttaccgctg aagattttca ggactatcag gccgttaaca agccactgtg
     5281 ggtggattat caaaacggcg cgatcacttc attacagctt cagcacgggc gtttcgagag
     5341 ctgggccgaa cggctgaaag ttgaagcagg cttgcttaac gatgccttta ttaatgcgat
     5401 ggcggaaatc tgcacgccgc tgccgggcgc ggtttctctg cttaacgcca ttcgtggcaa
     5461 cgccaaaatc ggcatcatca ccaacggctt tagcgccttg cagcaagtgc gtctggaacg
     5521 cacgggcctg cgtgattact tcgatttgct ggtgatttcc gaagaagttg gcgttgctaa
     5581 accgaataag aaaattttcg attatgcgct ggaacaggcg ggcaatcctg accgttcacg
     5641 cgtgctgatg gttggcgaca ctgccgagtc cgatattctc ggtggcatca acgccgggct
     5701 tgcgacctgc tggctgaatg cgcacaatcg cgagcaacca gaaggcatcg cgcccacctg
     5761 gaccgtttct tcgttgcacg aactggagca gctcctgtgt aaacactgat tgcctccccc
     5821 ccgttgatgg gtaaaatagc cgcaattttt cgttttcaac aagcgcggcg cgatgccgct
     5881 tactcaagaa gaaagaatta tgacgttgtc tccttatttg caagaggtgg ctaagcgccg
     5941 cacttttgcc attatttctc acccggacgc cggtaagact accatcaccg agaaggtgct
     6001 gctgttcgga caggccattc agaccgccgg tacagtaaaa ggccgtggtt ccaaccagca
     6061 cgctaagtcg gactggatgg agatggaaaa gcagcgtggg atctccatta ctacgtctgt
     6121 gatgcagttt ccgtatcacg attgcctggt taacctgcta gacaccccgg ggcacgaaga
     6181 cttctcggaa gatacctatc gtaccctgac ggcggtggac tgctgcctga tggttatcga
     6241 cgccgcaaaa ggtgttgaag atcgtacccg taagctgatg gaagttaccc gtctgcgcga
     6301 cacgccgatc ctcaccttta tgaacaaact tgaccgtgat atccgcgacc cgatggaact
     6361 gctcgatgaa gttgagaacg agctgaaaat cggctgcgca ccgatcacct ggccgattgg
     6421 ctgcggcaag ctgtttaaag gcgtttacca cctttataaa gacgaaacct atctctatca
     6481 gagcggtaaa ggtcacacca ttcaggaagt ccgcattgtt aaagggctga ataacccgga
     6541 tctcgatgct gcggttggtg aagatctggc gcagcagctg cgtgacgaac tggaactggt
     6601 gaaaggcgcg tctaacgagt tcgacaaaga attgttcctt gcgggcgaaa tcactccggt
     6661 attcttcggt actgcgctgg gtaacttcgg tgtcgatcat atgctggatg gcctggtgga
     6721 gtgggctcct gcgccgatgc cgcgtcagac tgatacccgt accgtagaag cgagcgaaga
     6781 caaatttacc ggcttcgtat ttaaaattca ggccaacatg gacccgaaac accgcgaccg
     6841 cgtggcgttt atgcgcgtgg tatccggtaa atatgaaaaa ggcatgaagc tgcgccaggt
     6901 gcgcaccgcg aaagacgtgg tgatctctga cgcgctgacc tttatggcgg gtgaccgttc
     6961 gcacgttgaa gaagcgtatc ctggcgatat cctcggcctg cacaaccacg gcaccattca
     7021 gatcggcgac acctttaccc agggtgagat gatgaagttc accggtattc cgaacttcgc
     7081 accagaactg ttccgtcgta tccgcctgaa agatccgctg aagcaaaaac agctgctcaa
     7141 agggctggta cagctttccg aagagggcgc ggtgcaggtg ttccgtccga tctccaacaa
     7201 cgatctgatc gttggtgcag ttggtgtgct gcagtttgat gtagtggtat cgcgcctgaa
     7261 gagcgaatac aacgttgaag cagtatatga atcagtcaac gttgccaccg cccgctgggt
     7321 agaatgtgca gacgcgaaga aattcgaaga gttcaagcgt aagaacgaaa gccaactggc
     7381 gcttgatggc ggcgataacc tcgcttacat cgctaccagc atggtcaacc tgcgcctggc
     7441 gcaggaacgt tatccggacg ttcagttcca ccagacccgc gagcattaat tcttgcctcc
     7501 agggcgcggt agccgctgcg ccctgtcaat ttcccttcct tattagccgc ttacggaatg
     7561 ttcttaaaac attcactttt gcttatgttt tcgctgatat cccgagcggt ttcaaaattg
     7621 tgatctatat ttaacaaagt aatgacattt ctgacggcgt taaataccgt tcaatgcgta
     7681 gatatcagta tctaaagccg tcgattgtca ttctaccgat attaataact gattcagagg
     7741 ctgtaatggt cgttattcat cacccatcgc ttttgtgatg gcgaccattg acttctgtag
     7801 agggtgaagt ctctccctat tcagcaatgc aacctcgtgt tgccaggctc aaattacgag
     7861 caaacataca ggaataaatc gatgactatg acaagactga agatttcgaa aactctgctg
     7921 gctgtaatgt tgacctctgc cgtcgcgacc ggctctgcct acgcggaaaa caacgcacag
     7981 actaccaatg aaagcgcagg gcaaaaagtc gatagctcta tgaataaagt cggtaatttc
     8041 atggatgaca gcgccatcac cgcgaaagtg aaggcggcgc tggtggatca tgacaacatc
     8101 aagagcaccg atatctctgt caaaaccgat caaaaagtcg tgaccctgag cggtttcgtt
     8161 gaaagccagg ctcaggccga agaggcagtg aaagtggcga aaggcgttga aggggtgacc
     8221 tctgtcagcg acaaactgca cgttcgcgac gctaaagaag gctctgtgaa gggctacgcg
     8281 ggtgacaccg ccaccaccag tgaaatcaaa gccaaactgc tggcggacga tatcgtccct
     8341 tcccgtcatg tgaaagttga aaccaccgac ggcgtggttc agctctccgg taccgtcgat
     8401 tcccaggcac agagcgaccg tgctgaaagt atcgccaaag cggtagatgg tgtgaaaagc
     8461 gttaaaaatg atctgaaaac taagtaattc gtcgtaattc gtcctcccga actttgtcgg
     8521 gaggcgcgat gtgcaccaca ctaaaaatat cgcattgagt gattttcacg ctcatatcaa
     8581 gcggtgacat taactatggt aaaggagacg cttatgtttc gttggggcat catatttctg
     8641 gttatcgcgt taatcgccgc cgcacttggg tttggtggtc tggccggtac cgctgcaggc
     8701 gcagctaaaa ttgtctttgt cgtcgggatt attctgttcc tggtgagttt gttcatgggc
     8761 cgaaaacgac cctagatttc agaccattaa gaaaatttta tccaaagcca gtccagcgga
     8821 ctggcttttg cggttttagc gaataataaa ttgcgttact ttgtcattct ttcacaacaa
     8881 aaacaggaag gcagaggtgg ggcagcgaat acctgtaacg cttggtaata ttgcgccgtt
     8941 gtcgctaagg ccgttccagc ctggacgaat agctctggtg tgcgaaggcg gcggacagcg
     9001 tggaattttc acggctggcg tgctggatga gtttatgcgc gcgcagttta atcctttcga
     9061 tctttatctc ggcacatctg ccggggcgca gaacctctcg gcgtttatct gcaatcagcc
     9121 cggttacgcg cgcaaagtca tcatgcgcta taccacaaaa cgcgaatttt tcgatccatt
     9181 gcgctttgtc cgtggaggaa atcttatcga tctcgactgg ctggtggagg ccaccgcaag
     9241 ccagatgccg ttgcaaatgg ataccgccgc gcggttgttt gacagcggca aatcgtttta
     9301 tatgtgcgcc tgtcgtcagg atgactacgc gcctaattac tttttaccaa ccaaacaaaa
     9361 ctggctggat gtgattcgcg cctccagtgc gatacctggc ttttatcgta gcggagtgtc
     9421 gctggaaggc attaactacc tggatggcgg gatcagtgat gcgattccgg ttaaagaggc
     9481 ggcaaggcag ggcgctaaaa cgttggtcgt catccgcact gtgccgtcac aaatgtacta
     9541 cacgccgcag tggttcaaac gcatggaacg ctggctgggt gacagtagcc tgcagccgct
     9601 ggtcaatctg gtgcagcatc atgaaaccag ctatcgtgac attcagcaat ttattgagaa
     9661 accaccgggc aagctgcgga tattcgaaat ttatccaccg aaaccattac atagtatcgc
     9721 gcttggcagt cggattccgg cgctgcgtga agactataaa cttgggcgtt tatgcggtcg
     9781 ttatttcctc gccacggttg gcaagctatt aactgaaaaa gcgccgctta cccgccatct
     9841 ggtgccagtg gtgacgccgg aatcgatcgt tattccgcct gcgccagtcg ccaacgatac
     9901 gctggttgcc gaagtgagcg atgctccgca ggcgaacgac ccgacattta acaatgagga
     9961 tctggcttga tttgccgttt tatcgatacc cactgccatt ttgatttccc gccgtttagt
    10021 ggcgatgaag aggccagcct gcaacgcgcg gcacaagcgg gcgtaggcaa gatcattgtt
    10081 ccggcgacag aggcggaaaa ttttgcccgc gtgcaggcgt tagcggaaaa atatcaaccg
    10141 ctgtatgccg cattggggtt gcatccgggg atgttggaaa aacatagcga tgtgtctctt
    10201 gatcagctac agcaggcgct ggaaaggcgt ccggcgaagg tggtggcggt gggggagatc
    10261 ggtctggatc tcttcggcga cgatccgcaa tttgagaggc agcagtggtt actcgacgaa
    10321 cagctgaaac tggcgaaacg ctacgatctg ccggtgatcc ttcattcccg gcgtacacac
    10381 gacaaactgg cgatgcatct taaacgtcac gatttaccgt gcactggcgt ggttcacggt
    10441 ttttccggca gcctgcaaca ggccgagcgg tttgtacagc tgggctacaa aatcggcgta
    10501 ggcggtacta tcacctatcc acgcgccagt aaaacccgcg atgtcatcgc aaaattaccg
    10561 ctggcatcgt tgttgctgga aaccgacgcg ccggatatgc cgctcaacgg ctttcagggg
    10621 cagcctaacc gaccggaaca ggctgtgcgg gtgtttgacg tgctttgcga gttgcgcccg
    10681 gaaccagaag atgagattgc cgaagtgttg cttaataata cgtatgcgtt gtttagcgtt
    10741 agtgggtagt ttacccttcg taggcagcat ccggcagtcg gcgcataatg cctgatgcga
    10801 cgcttgtcgc gtcttatcat gcctacaggt ttgtgccgaa ccgtaggccg gataaggcgt
    10861 tcacgccgca tccggcagtt ggcgcacaat gcctgatgcg acgcttgtcg cgtcttatca
    10921 tgcctacagg tttgtgccga accgtaggcc ggataaggcg ttcacgccgc atccggcagt
    10981 cggtgcataa tgcctgatgc gacgcttgtc gcgtcttatc atgcctacag gtttgtgccg
    11041 aaccgtaggc cggataaggc gttcacgccg catccggcag ttggcgcaca atgcctgatg
    11101 cgacgcttga cgcgtcttat catgcctaca agtctgtgcc gaaccgtagg ccggataagg
    11161 cgttcacgcc gcatccggca gtcggcgcat aatgcctgat gcgacgcttg tcgcgtctta
    11221 tcatgcctac aagtctgtgc cgaaccgtag gccgtatccg gcatgtcaca aatagagcgc
    11281 cggaaatatc aaccggctca ccccgcgcac ctttaacgca tcagccaacg gctcaacgtc
    11341 ttccggcgtg gcgctcgccc agctttgcgc ctcgccatac acgccgtggg catgaaacgc
    11401 gttcaggcgt accggaacat cgccaagtcc cttgataaac accgccagtt cttcgatgtg
    11461 ttgcaaataa tccacctggc cagggattac cagcaaacgc agttccgcca gcttaccgcg
    11521 ctctgccagc aaacagatgc tgcgcttaat ctgctgatta tcgcgtccgg ttaattgttg
    11581 atgacattcg ctcccccacg ctttgagatc gagcattgcg ccgtcacaca ccgggagtaa
    11641 tttttcccag ccggtttcgc tcaacatacc gttactgtcc acgaggcagg tgaggtggcg
    11701 cagttgcgga tcgtttttga tggctgtaaa cagcgccacc acaaacggca gctgggtcgt
    11761 ggcttcgccg ccgcttaccg ttatcccttc gataaacagc accgctttgc ggatatggct
    11821 aagcacctca tccacgctca tggattgcgc cattggcgtg gcatgttgcg gacacatctt
    11881 caggcaggta tcacactgct cgcagaccac agcgttccac agcactttgc cgtcaacaat
    11941 ctgcaacgcc tgatgcggac actgtggcac gcactctccg caatcattgc agcgtcccat
    12001 cgtccacgga ttgtgacagt ttttgcagcg cagattgcag ccctgcaaaa acagagccag
    12061 acgactgcct ggcccgtcaa cgcaggagaa ggggataatc ttactgacta aagcgcatct
    12121 gctgttcatg gcttatcacg cgcggctggc gttccagaat acgagtgttg cgtgcggctt
    12181 cttcgcccag ccaggtggtg ttggtgcgtg aaccttcggc gcgatatttt tctaaatccg
    12241 acaaacgcac catataaccg gtaacgcgaa ccagatcgtt accgctgaca ttggcggtaa
    12301 attcacgcat tccggcttta aaggcaccga ggcaaagctg taccagtgcc tgcgggttac
    12361 gtttgatggt ttcgtcgagg gtcagaatgt cgctgatgcc ggaataataa taagcatgat
    12421 gcggggcgac agtttgcaga tgggtgatcg gatctggctc atcgccatac ggcaaacgcg
    12481 cgcccggcgt ggtgccgata tcggaactga tccccgactg tgcgtgtaac atggcgcgtt
    12541 tttgccagcc atatttcacg ggggtattgg cgacaaactc cgccagttgc gcgctgatgc
    12601 gataacccac ttcatttgcg gcggcttctt taccgtagcg cgcggcaatc ccttctttct
    12661 cacacagcaa gttaaccgct tccgccagcc catacatgcc aaacattggc acaaaacgtt
    12721 cagggttaat cagcccttct ttcaccagga agctattctc aaagaagtgt gattgttgat
    12781 agaggaattc acaccgcgca tcgatgatgg cgatctgctg ctggcagtag tgcggtagag
    12841 tgcgcgtaaa gaagtcatcc agcgattcgc tgcgctcggc aatggctttc aggttaaggc
    12901 gtaccagcgt gctgccacca cccgccagcg gcagtgagtt gtaacagctc acaatcccgt
    12961 agcccccttt tgtgaaaatt ttatcatgca ccggaccgtt ggcgatgtgc ggtttgctac
    13021 attcacagat gttcttcgcc acttccagca gcaggtcatc aggggtgatt tctggatcgt
    13081 agataaaggt caggttcggt gaaacctgct tcaactctgc atctgcacgt aatatcgcac
    13141 gggtgatggg cgaatcagac gggccgatat tggcgtgcat aaaggcgtct ggcagggttc
    13201 tgtcgaggta acgccagaaa cgttttattc gaatatcgat ctcgtcttgt gttagaattc
    13261 taacatacgg ttgcaacaac gcatccagtt gccccaggta gaccggcatc gatgtgaccg
    13321 acggtacgtg gtggtaaaga atggtcagca gagagagtgc gtcatcaaga tctttcgcgc
    13381 cttccagctc cagccattcg gaaccgttcg ccagaaaacg ggcgtaatcg ggtaagacat
    13441 agcgcggttt gtacggcgca tgaccttcaa acatatcgca gattacaccc tcatccagcg
    13501 cgcggcgggc ctcggcaggc agctgcggat aaggcagatt gttttctgct tccagtgcca
    13561 gaaaatggcg cttctgctcc gggctaagca ctgggctggt gacaatttgc tggcaacgtt
    13621 gttgcagtgc attttcatga gaagtgggca tcttcttttc cttttatgcc gaaggtgatg
    13681 cgccattgta agaagtttcg tgatgttcac tttgatcctg atgcgtttgc caccactgac
    13741 gcattcattt gaaagtgaat tatttgaacc agatcgcatt acagtgatgc aaacttgtaa
    13801 gtagatttcc ttaattgtga tgtatatcga agtgtgttgc ggagtagatg ttagaatact
    13861 aacaaactcg caaggtgaat tttattggcg acaagccagg agaatgaaat gactgatctg
    13921 aaagcaagca gcctgcgtgc actgaaatta atggacctga ccaccctgaa tgacgacgac
    13981 accgacgaga aagtaattgc tctgtgtcat caggccaaaa ccccggtcgg caataccgcc
    14041 gctatctgta tctatcctcg ctttatcccg attgctcgca aaacgctgaa agagcagggc
    14101 accccggaaa tccgaatcgc tacggtaacc aacttcccac acggtaacga tgacatcgaa
    14161 atcgcgctgg cagaaacccg tgcggcaatc gcctacggtg cagatgaagt tgacgttgtg
    14221 ttcccgtacc gcgcgctgat ggcgggcaac gagcaggttg gttttgacct ggtgaaagcc
    14281 tgtaaagagg cttgcgcggc agcgaatgta ctgctgaaag tgatcatcga aaccggcgaa
    14341 ctgaaagacg aagcgctgat ccgtaaagcg tctgaaatct ccatcaaagc gggtgcggac
    14401 ttcatcaaaa cctctaccgg taaagtggct gtgaacgcga cgccggaaag cgcgcgcatc
    14461 atgatggaag tgatccgtga tatgggcgta gaaaaaaccg ttggtttcaa accggcgggc
    14521 ggcgtgcgta ctgcggaaga tgcgcagaaa tatctcgcca tcgcagatga actgtttggt
    14581 gctgactggg ctgatgcgcg tcactaccgc tttggtgctt ccagcctgct ggcaagcctg
    14641 ctgaaagcgc tgggtcacgg cgacggtaag agcgccagca gctactaagt aagatgcttt
    14701 acgcctgatg cgctgcgctt atcaggccta cgagacgtat ctacccgtag gccggataag
    14761 gcgtagacgc atccggcaaa agccgcctca tactcttttc ctcgggaggt taccttgttt
    14821 ctcgcacaag aaattattcg taaaaaacgt gatggtcatg cgctgagcga tgaagaaatt
    14881 cgtttcttta tcaacggtat tcgcgacaac actatctccg aagggcagat tgccgccctc
    14941 gcgatgacca ttttcttcca cgatatgaca atgcctgagc gtgtctcgct gaccatggcg
    15001 atgcgagatt caggaaccgt tctcgactgg aaaagcctgc atctgaatgg cccgattgtt
    15061 gataaacact cgaccggcgg cgtcggcgat gtgacttcgc tgatgttggg gccgatggtc
    15121 gcagcctgcg gcggctatat tccgatgatc tctggtcgcg gcctcggtca tactggcggt
    15181 acgctcgaca aactggaatc catccctggc ttcgacattt tcccggatga caaccgtttc
    15241 cgcgaaatta ttaaagacgt cggcgtggcg attatcggtc agaccagctc actggctccg
    15301 gcggataaac gtttctacgc gacccgtgat attaccgcaa ccgtggactc tatcccgctg
    15361 atcactgcct cgatcctggc gaagaaactg gcggaaggtc tggatgcgct ggtgatggac
    15421 gtgaaagtgg gtagcggcgc gtttatgccg acctacgaac tctctgaagc ccttgccgaa
    15481 gcgattgttg gcgtggctaa cggcgctggc gtgcgcacca ccgcgctgct caccgacatg
    15541 aatcaggtac tggcctccag tgcaggtaac gcggttgaag ttcgtgaagc ggtgcagttc
    15601 ctgacgggtg agtatcgtaa cccgcgtctg tttgatgtca cgatggcgct gtgcgtggag
    15661 atgctgatct ccggcaaact ggcgaaagat gacgccgaag cgcgcgcgaa attgcaggcg
    15721 gtgctggaca acggtaaagc ggcagaagtc tttggtcgta tggtagcggc acaaaaaggc
    15781 ccgaccgact tcgttgagaa ctacgcgaag tatctgccga cagcgatgct gacgaaagca
    15841 gtctatgctg ataccgaagg ttttgtcagt gaaatggata cccgcgcgct ggggatggca
    15901 gtggttgcaa tgggcggtgg acgccgtcag gcatctgaca ccatcgatta cagcgtcggc
    15961 tttactgata tggcgcgtct gggcgaccag gtagacggtc agcgtccgct ggcggttatc
    16021 cacgcgaaag acgaaaacag ctggcaggaa gcggcgaaag cggtgaaagc ggcaattaaa
    16081 cttgccgata aagcaccgga aagcacacca actgtctatc gtcgtatcag tgaataacgg
    16141 tatactgatc tgatcattta aatttgaagc actgagtacg gagaacatat gaaacgtgca
    16201 tttattatgg tgctggactc attcggcatc ggcgctacag aagatgcaga acgctttggt
    16261 gacgtcgggg ctgacaccct gggtcatatc gcagaagcct gtgccaaagg cgaagctgat
    16321 aacggtcgta aaggcccgct caatctgcca aatctgaccc gtctggggct ggcgaaagca
    16381 cacgaaggtt ctaccggttt cattccggcg ggaatggacg gcaacgctga agttatcggc
    16441 gcgtacgcat gggcgcacga aatgtcatcc ggtaaagata ccccgtctgg tcactgggaa
    16501 atcgccggtg tcccggttct gtttgagtgg ggatatttct ctgatcacga aaacagcttc
    16561 ccgcaagagc tgctggataa actggtcgaa cgcgctaatc tgccgggtta cctcggtaac
    16621 tgccactctt ccggtacggt cattctggat cagctgggcg aagagcacat gaaaaccggc
    16681 aagccgattt tctatacctc cgctgactct gtgttccaga ttgcctgcca cgaagaaacc
    16741 tttggcctgg ataaactcta cgaactgtgc gaaatcgccc gtgaagagct gaccaacggc
    16801 ggctacaaca tcggtcgtgt tatcgctcgt ccgtttatcg gtgacaaagc cggtaacttc
    16861 cagcgtaccg gtaaccgtca cgacctggct gttgagccgc cagcaccgac cgtgctgcag
    16921 aaactggttg atgaaaaaca cggtcaggtg gtttctgtcg gtaaaattgc cgacatctac
    16981 gccaactgcg gtatcaccaa gaaagtgaaa gcaactggcc tggacgcgct gtttgacgcc
    17041 accatcaaag agatgaaaga agcgggtgat aacactatcg tcttcaccaa cttcgttgac
    17101 ttcgactctt cttggggcca ccgtcgcgac gtcgccggtt atgctgcggg tctggagctg
    17161 ttcgaccgcc gtctgccgga gctgatgtct ctgctgcgcg atgacgacat cctgatcctc
    17221 actgctgacc acggttgtga tccgacctgg accggtactg accacacgcg tgaacacatt
    17281 ccggtactgg tttacggccc gaaagtaaaa ccgggctcac tgggtcaccg tgaaaccttc
    17341 gcggatatcg gccagactct ggcaaaatat tttggtactt ctgatatgga atatggcaaa
    17401 gccatgttct gataatgggt gcggcttgat gccctcaccc cggccctctc ccacagggag
    17461 agggtgaaaa catgtagata aggataaaac aatggctacc ccacacatta atgcagaaat
    17521 gggcgatttc gctgacgtag ttttgatgcc aggcgacccg ctgcgtgcaa agtatattgc
    17581 tgaaactttc cttgaagatg cccgtgaagt gaacaacgtt cgcggtatgc tgggcttcac
    17641 cggtacttac aaaggccgca aaatttccgt aatgggtcac ggtatgggta tcccgtcctg
    17701 ctccatctac accaaagaac tgatcaccga tttcggcgtg aagaaaatta tccgcgtggg
    17761 ttcctgtggc gcagttctgc cgcacgtaaa actgcgcgac gtcgttatcg gtatgggtgc
    17821 ctgcaccgat tccaaagtta accgcatccg ttttaaagat catgactttg ccgctatcgc
    17881 tgactttgac atggtgcgta acgcggtaga cgcggctaaa gcactgggcg ttgatgctcg
    17941 cgtgggtaac ctgttctccg ctgacctgtt ctactctccg gacggcgaaa tgttcgacgt
    18001 gatggaaaaa tacggcatcc tcggcgtgga aatggaagcg gctggtatct acggcgtggc
    18061 tgcagagttt ggcgcgaaag ccctgaccat ctgcaccgtg tctgaccaca tccgcactca
    18121 cgagcagacc actgccgctg agcgtcagac caccttcaac gacatgatca aaatcgcact
    18181 ggaatccgtt ctgctgggcg ataaagagta attgtgtttc gctgaaaggc gaacgtcgtg
    18241 ttaagccgga gcaggagact gctccggctt tttag
//
LOCUS       DAZKHF020000075        18240 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2201S31241
            SAMN43267800-rid25106413.denovo.075, whole genome shotgun sequence.
ACCESSION   DAZKHF020000075 DAZKHF020000000
VERSION     DAZKHF020000075.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267800
            Sequence Read Archive: SRR30307263
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 18240)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18240)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 18240)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350581.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350581.2
            Genome Coverage       :: 151x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:21:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,652
            CDSs (total)                      :: 5,557
            Genes (coding)                    :: 5,320
            CDSs (with protein)               :: 5,320
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 237
            CDSs (without protein)            :: 237
            Pseudo Genes (ambiguous residues) :: 0 of 237
            Pseudo Genes (frameshifted)       :: 102 of 237
            Pseudo Genes (incomplete)         :: 130 of 237
            Pseudo Genes (internal stop)      :: 52 of 237
            Pseudo Genes (multiple problems)  :: 41 of 237
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18240
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267800-rid25106413.denovo.075"
                     /serotype="O 26:H11"
                     /isolate="2201S31241"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-01"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            <1..104
                     /gene="rcbA"
                     /locus_tag="ACHOSC_RS10580"
                     /old_locus_tag="pgaptmp_002116"
     CDS             <1..104
                     /gene="rcbA"
                     /locus_tag="ACHOSC_RS10580"
                     /old_locus_tag="pgaptmp_002116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="double-strand break reduction protein RcbA"
                     /protein_id="HGU4548052.1"
                     /translation="ECEKMLSGKKEAGVSREQKVKLINFNCEKLLSS"
     gene            211..492
                     /locus_tag="ACHOSC_RS10585"
                     /old_locus_tag="pgaptmp_002117"
     CDS             211..492
                     /locus_tag="ACHOSC_RS10585"
                     /old_locus_tag="pgaptmp_002117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001443927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excisionase"
                     /protein_id="HGU4548053.1"
                     /translation="MNLVTLKTWGKLRYPDNPPSISTLRRWARNGNIYPAPELHGRSY
                     RVVPEAFYINPNKVDTDITHHQPNGRQGRDSPLLEKLKHAAEKIRSQFT"
     gene            458..1534
                     /locus_tag="ACHOSC_RS10590"
                     /old_locus_tag="pgaptmp_002118"
     CDS             458..1534
                     /locus_tag="ACHOSC_RS10590"
                     /old_locus_tag="pgaptmp_002118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001189081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HGU4548054.1"
                     /translation="MQRKKYDPNLPKNLTYRRRDKAYYWRNPLTKEEFTLGKISRRDA
                     VAQAIEANHYIYKNYSPAALIEKLKGFDSFTMADWIERYKTILIRRKVSRNTYKIRVN
                     QLETIKEKLGGVLLTEITTRHIAEFLDLWIEGGKNTMAGSMRSVLSDMFREAIVEGRI
                     SQNPVTPTRAPKIVVTRERLKLKTYNCIREAADQLPTWFPLAMDLALVTGQRREDITN
                     MRFSEIYDDRLHIRQIKTGMMIAIPLSLSLPVAGLRLGTVVEQCRMVSRGDYLISAGI
                     RKNSPDGSIHPDGLTKKFVAARKLTGIQFSENPPTFHEIRSLAGRLYKETCGEEFAQR
                     LLGHTSEKTTKMYLDEREKTYLLL"
     gene            complement(1927..2268)
                     /gene="yebY"
                     /locus_tag="ACHOSC_RS10595"
                     /old_locus_tag="pgaptmp_002119"
     CDS             complement(1927..2268)
                     /gene="yebY"
                     /locus_tag="ACHOSC_RS10595"
                     /old_locus_tag="pgaptmp_002119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebY family protein"
                     /protein_id="HGU4548055.1"
                     /translation="MMKKSILTFLLLTSSAAALAAPQVITVSRFEVGKDKWAFNREEV
                     MLTCRPGNALYVINPSTLVQYPLNDIAQKEVASGKTKAQPISVIQIDDPNNPGEKMSL
                     APFIERAEKLC"
     gene            complement(2281..3153)
                     /gene="copD"
                     /locus_tag="ACHOSC_RS10600"
                     /old_locus_tag="pgaptmp_002120"
     CDS             complement(2281..3153)
                     /gene="copD"
                     /locus_tag="ACHOSC_RS10600"
                     /old_locus_tag="pgaptmp_002120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006825 - copper ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="copper homeostasis membrane protein CopD"
                     /protein_id="HGU4548056.1"
                     /translation="MLAFTWIALRFIHFTSLMLVFGFAMYGAWLAPLTIRRLLAKRFL
                     RLQQHAAVWSLISATAMLAVQGGLMGTGWADVFSPNIWQAVLQTQFGGVWLWQIVLAL
                     VTLIVALMQPRNMPRLLFMLTTAQFILLAGIGHATLNEGVTAKIHQTNHAIHLICAAA
                     WFGGLLPVLWCMQLIKGRWRHQAIQALMRFSWCGHFAVIGVLASGVLNALLITGFPPT
                     LTTYWGQLLLLKAILVMIMVVIALANRYVLVPRMRQDEDRAAPWFEWMTKLEWAIGAV
                     VLVIISLLATLEPF"
     gene            complement(3157..3531)
                     /gene="yobA"
                     /locus_tag="ACHOSC_RS10605"
                     /old_locus_tag="pgaptmp_002121"
     CDS             complement(3157..3531)
                     /gene="yobA"
                     /locus_tag="ACHOSC_RS10605"
                     /old_locus_tag="pgaptmp_002121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005507 - copper ion binding [Evidence
                     IEA];
                     GO_process: GO:0006825 - copper ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CopC domain-containing protein YobA"
                     /protein_id="HGU4548057.1"
                     /translation="MTSTARSLRYALAILTTSLVTPSVWAHAHLTHQYPAANAQVTAA
                     PQAITLNFSEGVETGFSGAKITGPKNENIKTLPAKRNEQDQKQLIVPLADSLKPGTYI
                     VDWHVVSVDGHKTKGHYTFSVK"
     gene            3670..3900
                     /gene="holE"
                     /locus_tag="ACHOSC_RS10610"
                     /old_locus_tag="pgaptmp_002122"
     CDS             3670..3900
                     /gene="holE"
                     /locus_tag="ACHOSC_RS10610"
                     /old_locus_tag="pgaptmp_002122"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit theta"
                     /protein_id="HGU4548058.1"
                     /translation="MLKNLAKLDQTEMDKVNVDLAAAGVAFKERYNMPVIAEAVEREQ
                     PEHLRSWFRERLIAHRLASVNLSRLPYEPKLK"
     gene            4002..4658
                     /gene="yobB"
                     /locus_tag="ACHOSC_RS10615"
                     /old_locus_tag="pgaptmp_002123"
     CDS             4002..4658
                     /gene="yobB"
                     /locus_tag="ACHOSC_RS10615"
                     /old_locus_tag="pgaptmp_002123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon-nitrogen hydrolase family protein YobB"
                     /protein_id="HGU4548059.1"
                     /translation="MSFWKVAAAQYEPRKTSLTEQVAHHLEFVRAAARQQCQLLVFPS
                     LSLLGCDYSRRALPAPPDLSLLDPLCYAATTWRMTIIAGLPVEYNDRFIRGIAVFAPW
                     RKTPGIYHQSHGACLGRRSRTITVVDEQPQGMDMDPTCSLFTTGQCLGEPDLLASARR
                     LQFFSHQYSIAVLMANARGNSALWDEYGRLIVRADRGSLLLVGQRSSQGWQGDIIPLR
                     "
     gene            4682..5344
                     /gene="exoX"
                     /locus_tag="ACHOSC_RS10620"
                     /old_locus_tag="pgaptmp_002124"
     CDS             4682..5344
                     /gene="exoX"
                     /locus_tag="ACHOSC_RS10620"
                     /old_locus_tag="pgaptmp_002124"
                     /EC_number="3.1.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease X"
                     /protein_id="HGU4548060.1"
                     /translation="MLRIIDTETCGLQGGIVEIASVDVIDGKIVNPMSHLVRPDRPIS
                     PQAMAIHRITEAMVADKPWIEDVIPHYYGSEWYVAHNASFDRRVLPEMPGEWICTMKL
                     ARRLWPGIKYSNMALYKTRKLNVQTPPGLHHHRALYDCYITAALLIDIMNTSGWTAEQ
                     MADITGRPSLMTTFTFGKYRGKAVSDVAERDPGYLRWLFNNLDSMSPELRLTLKHYLE
                     NT"
     gene            complement(5341..7401)
                     /gene="ptrB"
                     /locus_tag="ACHOSC_RS10625"
                     /old_locus_tag="pgaptmp_002125"
     CDS             complement(5341..7401)
                     /gene="ptrB"
                     /locus_tag="ACHOSC_RS10625"
                     /old_locus_tag="pgaptmp_002125"
                     /EC_number="3.4.21.83"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptidase B"
                     /protein_id="HGU4548061.1"
                     /translation="MLPKAARIPHAMTLHGDTRIDNYYWLRDDTRSQPEVLDYLQQEN
                     SYGHRVMASQQALQDRILKEIIDRIPQREVSAPYIKNGYRYRHIYEPGCEYAIYQRQS
                     AFSEEWDEWETLLDANKRAAHSEFYSMGGMAITPDNTIMALAEDFLSRRQYGIRFRNL
                     ETGNWYPELLDNVEPSFVWANDSWTFYYVRKHPVTLLPYQVWRHAIGTPASQDKLIYE
                     EKDDTYYVSLHKTTSKHYVVIHLASATTSEVRLLDAEMADAEPFVFLPRRKDHEYSLD
                     HYQHRFYLRSNRHGKNFGLYRTRMRDEQQWEELIPPRENIMLEGFTLFTDWLVVEERQ
                     RGLTSLRQINRKTREVIGIAFDDPAYVTWIAYNPEPETARLRYGYSSMTTPDTLFELD
                     MDTGERRVLKQTEVPGFDAANYRSEHLWIVARDGVEVPVSLVYHRKHFRKGHNPLLVY
                     GYGSYGASIDADFSFSRLSLLDRGFVYAIVHVRGGGELGQQWYEDGKFLKKKNTFNDY
                     LDACDALLKLGYGSPSLCYAMGGSAGGMLMGVAINQRPELFHGVIAQVPFVDVVTTML
                     DESIPLTTGEFEEWGNPQDPQYYEYMKSYSPYDNVTAQAYPHLLVTTGLHDSQVQYWE
                     PAKWVAKLRELKTDDHLLLLCTDMDSGHGGKSGRFKSYEGVAMEYAFLVALAQGTLPA
                     TPAD"
     gene            complement(7610..8269)
                     /gene="yebE"
                     /locus_tag="ACHOSC_RS10630"
                     /old_locus_tag="pgaptmp_002126"
     CDS             complement(7610..8269)
                     /gene="yebE"
                     /locus_tag="ACHOSC_RS10630"
                     /old_locus_tag="pgaptmp_002126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tellurite resistance TerB family protein"
                     /protein_id="HGU4548062.1"
                     /translation="MANWLNQLQSLLGQSSSSTSSSADQGLGKLLVPGALGGLAGLLV
                     ANKSARKLLTKYGTNALLVGGGAVAGTVLWNKYKDKIRAAHQDEPQFGAQSTPLDERT
                     ERLILALVFAAKSDGHIDAKERAAIDQQLREAGVEEQGRVLIEQAIEQPLDPQRLATG
                     VRNEEEALEIYFLSCAAIDIDHFMERSYLNALGDALKIPQDVRDGIERDLEQQKRTLA
                     E"
     gene            complement(8596..8952)
                     /gene="yebF"
                     /locus_tag="ACHOSC_RS10635"
                     /old_locus_tag="pgaptmp_002127"
     CDS             complement(8596..8952)
                     /gene="yebF"
                     /locus_tag="ACHOSC_RS10635"
                     /old_locus_tag="pgaptmp_002127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310584.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YebF"
                     /protein_id="HGU4548063.1"
                     /translation="MKKRGAFLGLLLVSACASVFAANNETSKSVTFPKCEGLDAAGIA
                     ASVKRDYQQNRVARWADDQKIVGQADPVAWVSLQDIQGKDDKWSVPLTVRGKSADIHY
                     QVSVDCKAGMAEYQRR"
     gene            complement(9019..9309)
                     /gene="yebG"
                     /locus_tag="ACHOSC_RS10640"
                     /old_locus_tag="pgaptmp_002128"
     CDS             complement(9019..9309)
                     /gene="yebG"
                     /locus_tag="ACHOSC_RS10640"
                     /old_locus_tag="pgaptmp_002128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA damage-inducible protein YebG"
                     /protein_id="HGU4548064.1"
                     /translation="MAVEVKYVVIREGEEKMSFTSKKEADAYDKMLDTADLLDTWLTN
                     SPVQMEDEQREALSLWLAEQKDVLSTILKTGKLPSPQVVGAESEEEDASHAA"
     gene            9443..10621
                     /gene="purT"
                     /locus_tag="ACHOSC_RS10645"
                     /old_locus_tag="pgaptmp_002129"
     CDS             9443..10621
                     /gene="purT"
                     /locus_tag="ACHOSC_RS10645"
                     /old_locus_tag="pgaptmp_002129"
                     /EC_number="2.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004644 - phosphoribosylglycinamide
                     formyltransferase activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate-dependent phosphoribosylglycinamide
                     formyltransferase"
                     /protein_id="HGU4548065.1"
                     /translation="MTLLGTALRPAATRVMLLGSGELGKEVAIECQRLGVEVIAVDRY
                     ADAPAMHVAHRSHVINMLDGDALRRVVELEKPHYIVPEIEAIATDMLIQLEEEGLNVV
                     PCARATKLTMNREGIRRLAAEQLQLPTSTYRFADSESLFREAVAAIGYPCIVKPVMSS
                     SGKGQTFIRSAEQLAQAWEYAQQGGRAGAGRVIVEGVVKFDFEITLLTVSAVDGVHFC
                     APVGHRQEDGDYRESWQPQQMSPLALERAQEIARKVVLALGGYGLFGVELFVCGDEVI
                     FSEVSPRPHDTGMVTLISQDLSEFALHVRAFLGLPVGGIRQYGPAASAVILPQLTSQN
                     VTFDNVQNAVGAGLQIRLFGKPAINGSRRLGVALATAESVVDAIKRAKHAAGQVKVQG
                     "
     gene            complement(10677..11318)
                     /gene="kdgA"
                     /locus_tag="ACHOSC_RS10650"
                     /old_locus_tag="pgaptmp_002130"
                     /gene_synonym="eda"
     CDS             complement(10677..11318)
                     /gene="kdgA"
                     /locus_tag="ACHOSC_RS10650"
                     /old_locus_tag="pgaptmp_002130"
                     /gene_synonym="eda"
                     /EC_number="4.1.2.14"
                     /EC_number="4.1.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 4-hydroxy-2-oxoglutarate
                     aldolase/2-dehydro-3-deoxy-phosphogluconate aldolase"
                     /protein_id="HGU4548066.1"
                     /translation="MKNWKTSAESILTTGPVVPVIVVKKLEHAVPMAKALVAGGVRVL
                     EVTLRTECAVDAIRAIAKEVPEAIVGAGTVLNPQQLAEVTEAGAQFAISPGLTEPLLK
                     AATEGTIPLIPGISTVSELMLGMDYGLKEFKFFPAEANGGVKALQAIAGPFSQVRFCP
                     TGGISPANYRDYLALKSVLCIGGSWLVPADALEAGDYDRITKLAREAVEGAKL"
     gene            complement(11355..13166)
                     /gene="edd"
                     /locus_tag="ACHOSC_RS10655"
                     /old_locus_tag="pgaptmp_002131"
     CDS             complement(11355..13166)
                     /gene="edd"
                     /locus_tag="ACHOSC_RS10655"
                     /old_locus_tag="pgaptmp_002131"
                     /EC_number="4.2.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004456 - phosphogluconate dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009255 - Entner-Doudoroff pathway through
                     6-phosphogluconate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphogluconate dehydratase"
                     /protein_id="HGU4548067.1"
                     /translation="MNPQLLRVTNRIIERSRETRSAYLARIEQAKTSTVHRSQLACGN
                     LAHGFAACQPEDKASLKSMLRNNIAIITSYNDMLSAHQPYEHYPEIIRKALHEANAVG
                     QVAGGVPAMCDGVTQGQDGMELSLLSREVIAMSAAVGLSHNMFDGALFLGVCDKIVPG
                     LTMAALSFGHLPAVFVPSGPMASGLPNKEKVRIRQLYAEGKVDRMALLESEAASYHAP
                     GTCTFYGTANTNQMVVEFMGMQLPGSSFVHPDSPLRDALTAAAARQVTRMTGNGNEWM
                     PIGKMIDEKVVVNGIVALLATGGSTNHTMHLVAMARAAGIQINWDDFSDLSDVVPLMA
                     RLYPNGPADINHFQAAGGVPVLVRELLKAGLLHEDVNTVAGFGLSRYTLEPWLNNGEL
                     DWREGAEKSLDSNVIASFEQPFSHHGGTKVLSGNLGRAVMKTSAVPVENQVIEAPAVV
                     FESQHDVMPAFEAGLLDRDCVVVVRHQGPKANGMPELHKLMPPLGVLLDRCFKIALVT
                     DGRLSGASGKVPSAIHVTPEAYDGGLLAKVRDGDIIRVNGQTGELTLLVDEAELAARE
                     PHIPDLSASRVGTGRELFSALREKLSGAEQGATCITF"
     gene            complement(13401..14876)
                     /gene="zwf"
                     /locus_tag="ACHOSC_RS10660"
                     /old_locus_tag="pgaptmp_002132"
     CDS             complement(13401..14876)
                     /gene="zwf"
                     /locus_tag="ACHOSC_RS10660"
                     /old_locus_tag="pgaptmp_002132"
                     /EC_number="1.1.1.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005120712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004345 - glucose-6-phosphate
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0016614 - oxidoreductase activity, acting
                     on CH-OH group of donors [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0006006 - glucose metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate dehydrogenase"
                     /protein_id="HGU4548068.1"
                     /translation="MAVTQTAQACDLVIFGAKGDLARRKLLPSLYQLEKAGQLNPDTR
                     IIGVGRADWDKAAYTKVVREALETFMKETIDEGLWDTLSARLDFCNLDVNDTAAFSRL
                     GAMLDQKNRITINYFAMPPSTFGAICKGLGEAKLNAKPARVVMEKPLGTSLATSQEIN
                     DQVGEYFEECQVYRIDHYLGKETVLNLLALRFANSLFVNNWDNRTIDHVEITVAEEVG
                     IEGRWGYFDKAGQMRDMIQNHLLQILCMIAMSPPSDLSADSIRDEKVKVLKSLRRIDR
                     SNVREKTVRGQYTAGFAQGKKVPGYLEEEGANKSSNTETFVAIRVDIDNWRWAGVPFY
                     LRTGKRLPTKCSEVVVYFKTPELNLFKESWQDLPQNKLTIRLQPDEGVDIQVLNKVPG
                     LDHKHNLQITKLDLSYSETFNQTHLADAYERLLLETMRGIQALFVRRDEVEEAWKWVD
                     SITEAWAMDNDAPKPYQAGTWGPVASVAMITRDGRSWNEFE"
     gene            15214..16083
                     /gene="hexR"
                     /locus_tag="ACHOSC_RS10665"
                     /old_locus_tag="pgaptmp_002133"
                     /gene_synonym="yebK"
     CDS             15214..16083
                     /gene="hexR"
                     /locus_tag="ACHOSC_RS10665"
                     /old_locus_tag="pgaptmp_002133"
                     /gene_synonym="yebK"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator HexR"
                     /protein_id="HGU4548069.1"
                     /translation="MNMLEKIQSQLEHLSKSERKVAEVILASPDNAIHSSIAALALEA
                     NVSEPTVNRFCRSMDTRGFPDFKLHLAQSLANGTPYVNRNVNEDDSVESYTGKIFESA
                     MATLDHVRHSLDKSAINRAVDLLTQAKKIAFFGLGSSAAVAHDAMNKFFRFNVPVVYS
                     DDIVLQRMSCMNCSDGDVVVLISHTGRTKNLVELAQLARENDAMVIALTSAGTPLARE
                     ATLAITLDVPEDTDIYMPMVSRLAQLTVIDVLATGFTLRRGAKFRDNLKRVKEALKES
                     RFDKQLLNLSDDR"
     gene            16211..17653
                     /gene="pyk"
                     /locus_tag="ACHOSC_RS10670"
                     /old_locus_tag="pgaptmp_002134"
     CDS             16211..17653
                     /gene="pyk"
                     /locus_tag="ACHOSC_RS10670"
                     /old_locus_tag="pgaptmp_002134"
                     /EC_number="2.7.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005120709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004743 - pyruvate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0030955 - potassium ion binding [Evidence
                     IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate kinase"
                     /protein_id="HGU4548070.1"
                     /translation="MSRRLRRTKIVTTLGPATDRDNNLEKVIAAGANVVRMNFSHGSP
                     EDHKMRADKVREIAAKLGRHVAILGDLQGPKIRVSTFKEGKVFLNIGDKFLLDANLGK
                     GEGDKEKVGIDYKGLPADVVPGDILLLDDGRVQLKVLEVQGMKVFTEVTVGGPLSNNK
                     GINKLGGGLSAEALTEKDKADIKTAALIGVDYLAVSFPRCGEDLNYARRLARDAGCDA
                     KIVAKVERAEAVCSQDAMDDIILASDVVMVARGDLGVEIGDPELVGIQKALIRRARQL
                     NRAVITATQMMESMITNPMPTRAEVMDVANAVLDGTDAVMLSAETAAGQYPSETVAAM
                     ARVCLGAEKIPSINVSKHRLDVQFDNVEEAIAMSAMYAANHLKGVTAIITMTESGRTA
                     LMTSRISSGLPIFAMSRHERTLNLTALYRGVTPVHFDSANDGVAAASEAVNLLRDKGY
                     LMSGDLVIVTQGDVMSTVGSTNTTRILTVE"
     gene            complement(17713..>18240)
                     /locus_tag="ACHOSC_RS10675"
                     /old_locus_tag="pgaptmp_002135"
     CDS             complement(17713..>18240)
                     /locus_tag="ACHOSC_RS10675"
                     /old_locus_tag="pgaptmp_002135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016243610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Nif3-like dinuclear metal center hexameric
                     protein"
                     /protein_id="HGU4548071.1"
                     /translation="KEFLHKNNLVIIRCHDVMDREPTFGMSKALAQQLELDVTNIVAS
                     DDMYHVYAIEPDSAINITKRFAKNLKIYNLPGIQFYGDRARIVRTIGIGAGCFCDPIQ
                     YMEYQADYYITINDSIKTWVQTQYSKDSGLPMAVIGYSVAEEAGMRRLASYLDLHSGY
                     PCIHFTGGCDYDWIE"
BASE COUNT         4685 a         4572 c         4427 g         4556 t
ORIGIN      
        1 cagaatgcga aaaaatgctc tcagggaaaa aagaagcagg cgtgtccaga gagcagaaag
       61 taaagctgat aaattttaat tgcgagaaac ttctgtcctc gtgagttgca ttatatacaa
      121 attagaactt catagctgat tattaaaaat caaccacacc cgccagtatt ctgtatattt
      181 actggcggtc atatcgtaag aggtatggca atgaatcttg tgacactcaa aacgtgggga
      241 aaactcagat atccggataa cccaccatca atatcaacgc tgagacgatg ggcaaggaat
      301 ggaaacattt atcctgcacc tgaactacac gggaggagtt acagggtggt tccggaagct
      361 ttctatatca acccaaataa ggttgatacc gatataacac accatcagcc taatgggcga
      421 caagggagag acagtccgtt actggagaag ttaaaacatg cagcggaaaa aatacgatcc
      481 caatttacct aaaaacttaa catatcgaag gagggacaaa gcatattact ggcgcaaccc
      541 tctgacgaaa gaagaattta cactaggtaa aatttcaaga agagatgcag tagcgcaggc
      601 aattgaagca aatcattata tatacaaaaa ctactctcct gctgccttaa ttgaaaaact
      661 aaaagggttc gactcattta ctatggcaga ctggattgaa cgttacaaaa cgattcttat
      721 aaggagaaaa gtgtccagaa atacttataa aattcgggta aatcaactgg agacaataaa
      781 agaaaaatta ggaggggttt tactgacaga aataaccact cgccatattg ccgagtttct
      841 tgatttgtgg attgaaggag ggaaaaacac gatggcagga tcaatgcgtt ctgtgttatc
      901 tgatatgttc cgcgaggcca ttgttgaagg acgtatatct caaaatccag taacgccaac
      961 aagagcaccg aaaatagtag ttacaagaga acggctgaaa ctaaagacat acaactgcat
     1021 cagggaggca gcagatcaac ttccgacatg gttcccatta gctatggact tagcccttgt
     1081 aacaggacaa cgtcgcgaag acataacaaa tatgcggttc agtgagattt atgatgatcg
     1141 tctccacatc aggcaaatta agacaggaat gatgattgct atccccctgt cactcagcct
     1201 tcctgtcgct ggtttacgac ttggtacagt agttgaacag tgccgcatgg taagtagggg
     1261 ggattatcta atcagtgccg ggattagaaa aaacagccct gacggcagca ttcacccgga
     1321 cggcctgaca aagaaatttg tcgcagccag aaaattaaca ggtatccagt tcagtgaaaa
     1381 cccaccaact tttcacgaga tcagaagcct ggctggacga ttgtacaaag aaacatgtgg
     1441 ggaagaattt gctcaacgtc tacttggcca cacatcggag aagacaacaa aaatgtatct
     1501 tgatgagaga gaaaaaacgt acttactgct ctgattttaa cgtaaatgga ttgttaaatg
     1561 tgttttggtt gtgatataac caaaaaagac cggaatacag aaattcgagt aaatttcgag
     1621 taatttcggg gagacgtttg caactaattg attttaaata caattaaaaa aagaccgaat
     1681 acgattcctg tattcggtcc agggaaatgg ctcttgggag agagccgtgc gctaaaagtt
     1741 ggcattaatg caggcttagt tgccttgccc tttaagaata gatgacgacg ccaggttttc
     1801 cagtttgcgt gcaaaatggt caataaaaag cgtggtggtc atcagctgaa atgttaaaaa
     1861 ccgcccgttc tggtgaaaga actgaggcgg tttttttatt ggaaatcaaa aggctatttt
     1921 aggtaattaa cagagttttt cagctcgttc tataaacggt gccagactca ttttttcgcc
     1981 gggattgtta ggatcatcaa tctgaatcac cgaaatgggt tgggctttag tcttcccact
     2041 ggcaacttcc ttttgtgcga tatcgtttaa aggatactgc acgagggtac ttgggttaat
     2101 gacatacaaa gcattacccg gtctgcaagt cagcatcacc tcttcgcgat taaacgccca
     2161 tttgtcttta cccacttcaa aacgactgac ggtaatcacc tgcggtgcag ccagcgccgc
     2221 tgcagaactg gtgagtaaca gaaacgtcag aatacttttt ttcatcattt tcgtaatcca
     2281 tcaaaaaggt tcgagagtcg caagcaggct gatgatcacc agcactaccg cacctatcgc
     2341 ccattcgagt ttagtcatcc actcaaacca gggtgccgca cgatcttcat cttgcctcat
     2401 gcgcggtaca agaacataac gattagccag cgcaatgacc accataatca tcaccaggat
     2461 ggctttgagc aataagagtt ggccccagta agtcgtaagc gtggggggaa atccggtaat
     2521 caacaatgca ttaagcacgc cgcttgccag tacgccgatc accgcaaaat gcccgcacca
     2581 ggaaaaacgc atcagcgcct gaatggcctg gtgtcgccaa cggcctttga tgagttgcat
     2641 acaccagagc accggcagta atcccccaaa ccacgccgcc gcacaaatca ggtgaatcgc
     2701 gtgattagtc tgatggattt tcgccgttac cccttcattc agtgtcgcat gcccgattcc
     2761 cgccagcaga ataaattgcg cggtagtgag cataaacagc aaacgtggca tattccgcgg
     2821 ttgcataagg gcgacgatca acgttacgag ggcgagaaca atttgccata accagacgcc
     2881 accaaactgc gtttgcaaaa cagcctgcca aatgtttggc gaaaatacat ctgcccatcc
     2941 cgttcccatc aacccgccct gaacggcgag cattgcagtg gcactgatca aactccagac
     3001 ggcagcgtgc tgttgtaaac gcagaaaacg cttcgccagc aagcgacgaa tcgttaaggg
     3061 ggccagccag gcaccgtaca ttgcgaagcc aaagaccagc atcagcgagg tgaaatggat
     3121 aaatcgcagc gcgatccagg taaacgccag catgatttat ttcacgctaa aggtgtagtg
     3181 tcctttcgtt ttatgcccgt ccaccgaaac aacatgccag tcaacgatgt aggttccggg
     3241 tttcagcgaa tcggccagtg gaacaatcag ttgcttttgg tcctgctcat ttcgcttcgc
     3301 cggtaatgtt ttaatatttt cgttttttgg ccccgtgatt tttgcaccac tgaatcctgt
     3361 ttcaacacct tccgagaagt ttaaagtgat tgcctgcggt gcagctgtca cttgcgcgtt
     3421 tgccgcagga tactgatgcg ttaaatgcgc atgtgcccag acggaaggtg tcaccaacga
     3481 agtggtcagg attgccagcg cgtagcgaag ggagcgtgca gttgaagtca tattatctat
     3541 tcctttttgt aataactttt ttacagagca taaccttgtc taatgtctga gtcgaggatc
     3601 atcaattccg gcttgccatc ctggctcact cttagtaact tttgcccgcg aatgatgagg
     3661 agattaagaa tgctgaagaa tctggctaaa ctggatcaaa cagaaatgga taaagtgaat
     3721 gtcgatttgg cggcggccgg ggtggcattt aaagaacgct acaatatgcc ggtgatcgct
     3781 gaagcggttg aacgtgaaca gcctgaacat ttgcgcagct ggtttcgcga gcggcttatt
     3841 gcccaccgtt tggcttcggt caatctgtca cgtttacctt acgagcccaa acttaaataa
     3901 aacttataca gagttacact ttcttacata acgcctgcta aattatgagt attttctaaa
     3961 ccgcacccat aatttgcagt cattttgaaa aggaagtcat tatgtcgttc tggaaagttg
     4021 cagcagcgca atatgagcct cgtaaaacct cactcaccga acaggtagct catcatctgg
     4081 agtttgtgag ggccgccgcc aggcaacaat gtcagctgtt agtttttcca tcgctttctt
     4141 tactggggtg tgattattcg cgacgtgccc ttcctgcccc acccgatctc tcactgttag
     4201 acccgctttg ttatgccgca acgacctggc gaatgaccat cattgccggc cttcccgttg
     4261 aatataacga tcgctttatt cgtggaattg cggtgttcgc accgtggcga aaaacgcctg
     4321 ggatctacca tcagagtcat ggtgcatgtc tgggtcgtcg ttccagaacc atcacggtgg
     4381 tcgatgaaca accgcaaggt atggatatgg acccgacctg ttcactgttt actacagggc
     4441 aatgtctggg agaacccgac ctgctggcgt cggcccgtcg cttacaattt ttttcacatc
     4501 agtactcgat tgccgtgctg atggcaaatg cccgtggtaa cagtgcacta tgggatgaat
     4561 atggtcgtct catcgttcgc gccgatcgtg gttcattatt gttagtcggt cagcgttcat
     4621 cacagggttg gcaaggcgat atcattccat tacgctaggc tttttcggcc tggagcatgc
     4681 catgttgcgc attatcgata cagaaacctg cggtttgcag ggagggatcg ttgagattgc
     4741 ctctgttgat gtcattgacg gaaaaatcgt caaccccatg agccacctgg tgcgccccga
     4801 tcgtcctatt agtccacaag cgatggcgat tcatcgcatc accgaagcca tggtcgccga
     4861 taaaccgtgg attgaagatg tgatcccaca ctattacggt agtgaatggt atgtcgcgca
     4921 taacgccagc tttgaccgcc gcgtactgcc tgagatgccc ggtgagtgga tttgcactat
     4981 gaaactggcc cgtcgtttgt ggcctgggat caagtacagc aatatggcgt tatataaaac
     5041 acgcaagctc aatgtacaga cgccgccggg cctgcatcat caccgcgcgt tgtatgactg
     5101 ttatatcacc gccgcgttgc ttatcgatat tatgaacacc tccggctgga cggcagaaca
     5161 gatggccgat atcaccggac gtccgtcgtt gatgacgacc ttcacctttg gcaaataccg
     5221 tggcaaagcg gtttccgacg ttgccgaacg cgatccgggc tatctgcgct ggttatttaa
     5281 taacctggac agcatgagcc cggagctgcg tttaacactg aaacattatc tggaaaatac
     5341 ttagtccgca ggcgtagcgg gtaatgttcc ctgcgccagc gcgaccagaa aagcatattc
     5401 catcgctacg ccttcgtacg atttaaagcg accagattta ccgccatggc ctgagtccat
     5461 gtcggtacag agcaataaaa gatggtcatc ggttttcagc tcgcgcaatt tagcgaccca
     5521 ttttgccggt tcccaatatt gcacctgaga atcgtgcaaa ccggtcgtta ccagtaaatg
     5581 cggataagcc tgtgcggtga cgttgtcata cgggctgtag cttttcatgt actcgtaata
     5641 ttgcggatcc tgcggattac cccactcttc aaactcacca gtggtaagag gaattgattc
     5701 atcaagcatc gttgttacaa catcaacaaa cggtacctgg gcgataacgc cgtggaataa
     5761 ttccgggcgt tgattaattg caacgcccat caacatgccc cccgcactcc cgcccatcgc
     5821 ataacaaagc gaaggagagc catagcccag ttttaacaat gcatcgcagg catcaagata
     5881 atcattaaac gtatttttct tcttcagaaa ttttccgtct tcgtaccatt gttgccccag
     5941 ctcaccaccg ccgcgaacat ggacaatggc gtagacaaag ccacgatcta acaaactcaa
     6001 gcggctaaaa ctgaaatcgg catcaatact tgcgccgtaa gaaccatagc catacaccag
     6061 caacgggttg tgtcctttgc gaaaatgttt gcgatggtag accaacgaaa ccggaacttc
     6121 gacgccatca cgggcgacta tccacaggtg ttcactgcgg taattcgccg catcaaaacc
     6181 gggcacttct gtttgtttta atacacgacg ctcaccggta tccatatcca gttcaaacaa
     6241 agtgtctggt gtggtcatgg aagaataacc ataacgcaat cgcgcggttt caggttctgg
     6301 attgtaggca atccaggtca cataggctgg atcatcaaag gcaataccaa tgacttcccg
     6361 ggtcttgcgg ttaatttggc gcaaactggt taacccgcgc tgacgctctt caaccaccag
     6421 ccagtcggta aacagcgtaa acccttccag catgatgttt tcgcgtggcg gaattaactc
     6481 ttcccactgt tgctcatcac gcatacgggt acggtataag ccaaagtttt tgccgtggcg
     6541 gttggaacgt agataaaaac gatgctggta gtgatcaagg ctgtattcgt gatctttgcg
     6601 gcgcggcaga aaaacaaacg gctcggcatc ggccatttcc gcgtccagca ggcgaacttc
     6661 actggtggtg gcgctggcca aatgaatgac tacatagtgc ttcgaggtcg ttttatgcag
     6721 gctgacgtaa taggtatcgt ctttttcttc gtagatcagt ttatcttgcg atgctggcgt
     6781 accgatggcg tgacgccaga cctgataagg cagcagcgtc accggatgct tgcgaacata
     6841 gtagaaagtc caggagtcat ttgcccagac aaagctgggt tcaacgttat ccagcagttc
     6901 cgggtaccag ttaccagttt ccagattacg aaaacgaatg ccgtactggc gtcgggaaag
     6961 aaaatcttct gccagcgcca taatggtgtt atcgggcgta atcgccattc cgcccatcga
     7021 ataaaactca ctatgagctg cgcgcttatt ggcatcgagc aatgtttccc actcatccca
     7081 ctcttcactg aatgccgatt gacgctggta gatagcatat tcacagcctg gttcataaat
     7141 atgccgatag cggtagccat ttttgatgta gggcgcagaa acttctcgtt gcggaatgcg
     7201 gtcgatgatt tcctttaaga tgcgatcctg caaggcttgt tgtgaggcca tcacccgatg
     7261 accgtaacta ttttcttgtt gcaggtagtc caggacttct ggctgagaac gcgtatcgtc
     7321 ccgcagccag tagtaattat cgatgcgcgt atcgccatga agcgtcatgg cgtggggaat
     7381 gcgggcggct tttggtagca tgttattgtt ctttctggtt gaaacatctt ataagggtgg
     7441 caaaactcac cggggatgca agcgaaacag gggagtcatt gcttagatga tgacaggtaa
     7501 tggcgcggat atcgaatgtt atgcagacag agaaaatcag cctgttcaaa tggctgtgcg
     7561 attctggata gcccgaaata gtcaacttca ggctatccag agagcggaat tattccgcca
     7621 aagtgcgttt ttgctgttcg agatcgcgtt caatgccgtc acgaacatcc tgggggattt
     7681 tcagcgcgtc acccagtgca ttcaggtaac tgcgttccat aaaatggtca atatcaatag
     7741 ccgcgcaact caggaaatag atttccagcg cctcttcttc attgcggacc ccggtagcca
     7801 ggcgttgtgg atccagcggt tgttcgattg cctgctcaat gagtacacgc ccctgctctt
     7861 ccacgccggc ttcacgcaat tgctggtcga ttgccgcacg ttctttggca tcaatatgac
     7921 catcactttt agcggcaaag accagcgcaa ggatcaaacg ttctgtacgc tcatccagcg
     7981 gcgtactttg cgcgccaaac tgcggttcgt cctgatgcgc cgcgcgaatt ttatctttgt
     8041 atttattcca cagcaccgta cccgctaccg ctccgccgcc aaccagtaac gcgttggtgc
     8101 catatttcgt aagaagttta cgtgctgatt tatttgcgac cagcagccca gccagtccgc
     8161 ctaatgcgcc tggcactaac agtttgccca atccctgatc cgcagacgag gaggtagaag
     8221 aactgctttg cccaagcagg gattgcagtt gatttaacca gttagccata tttgctcctc
     8281 aataaccatt tttagtgcct gtagcatagc ggatgaggat gtcagaaaat gtatatcgag
     8341 acaaaagatg cgcaaataag cggggcggta ggccgttatt caaagaaatt cgcgccaggt
     8401 aaggtattta ctgtatatca ggtgataagg gtattttagg tgaaagtagt ttgtcgtttt
     8461 gagtggcatt gtttgatgct gattgcccga tgcgacgctg ttgcgtcttc tcaggcctac
     8521 gattgtggtg cgttctgggg cggacaaggc actcgcgccg catccgccaa cggtcaatcg
     8581 aattatcaga agccattaac gccgctgata ttccgccatt cccgctttgc agtccacgct
     8641 gacctggtaa tgaatatcgg cacttttacc acgcacggtt agcggtactg accatttatc
     8701 atctttaccc tgaatgtcct gcaaactgac ccaagccacg ggatcggcct gaccgacaat
     8761 tttttgatca tctgcccaac gcgccacgcg attttgttga taatcacgtt ttacgctcgc
     8821 ggcaattccg gcagcatcca gaccttcaca ctttgggaaa gtgaccgact tgctggtttc
     8881 attattggca gcgaaaactg atgcgcaggc agaaaccaac aacagcccta aaaacgcccc
     8941 tctttttttc atgtttttct ccatagcaca atgattcagg agaaagcatg gtacaaattg
     9001 tcaggagcgc aagttgcttc aggccgcgtg tgaggcatct tcctcttcgg attcagcacc
     9061 gacgacctgt ggggacggta atttgccggt tttcagaatg gtgctcagca catctttttg
     9121 ttctgccagc catagcgaaa gggcttcacg ttgctcgtct tccatttgca ctggagaatt
     9181 tgtcagccag gtgtcaagca gatccgccgt atcaagcatt ttatcataag catcggcttc
     9241 ctttttgctg gtaaacgaca ttttctcttc gccctcacga atgactacgt atttaacttc
     9301 aaccgccatt tgcagcctct cataataact gtgattttat acagtatatt tcttttcggt
     9361 tgagaaatca acatcagcaa taaagacaca cgcaaacgtt ttcgtttata ctgcgcgcgg
     9421 aattaatcag gggatattcg ttatgacgtt attaggcact gcgctgcgtc cggcagcaac
     9481 tcgcgtgatg ttattaggct ccggtgaact gggtaaagaa gtggcaatcg agtgtcagcg
     9541 tctcggcgta gaggtgattg ccgtcgatcg ctatgccgac gcaccagcca tgcatgtcgc
     9601 gcatcgctcc catgtcatta atatgcttga tggtgatgca ttacgccgtg tggttgaact
     9661 ggaaaaacca cattatatcg tgccggagat cgaagctatt gccaccgata tgctgatcca
     9721 acttgaagag gaaggactga atgttgtccc ctgcgctcgc gcaacgaaat taacgatgaa
     9781 tcgcgagggt atccgtcgcc tggcggcaga acagctgcag ctgcccactt ccacttatcg
     9841 ttttgccgat agcgaaagcc ttttccgcga ggcggttgcc gccattggct atccctgcat
     9901 tgtaaaaccg gtgatgagct cttccggcaa ggggcagacg tttattcgct ctgcagagca
     9961 acttgctcag gcatgggaat acgctcagca aggcggtcgc gccggagcgg gccgcgtaat
    10021 tgttgaaggc gtcgttaagt ttgacttcga aattaccctg ctaaccgtca gcgcggtgga
    10081 tggcgtccat ttctgtgcac cagtaggtca tcgccaggaa gatggcgact accgtgaatc
    10141 ctggcaacca cagcaaatga gcccgcttgc ccttgaacgt gcgcaggaga ttgcccgtaa
    10201 agtggtgctg gcactgggcg gttatggtct gtttggtgtc gagctgtttg tctgtggtga
    10261 tgaggtgatt ttcagtgagg tctcccctcg tccacatgat accgggatgg tgacgttaat
    10321 ttctcaagat ctctcagagt ttgccctgca tgtacgtgcc ttcctcggac ttccggttgg
    10381 cgggatccgc cagtatggtc ctgcagcttc tgccgttatc ctgccacaac tgaccagtca
    10441 gaatgtcacg tttgataatg tgcagaatgc cgtaggcgca ggtttgcaga ttcgtttatt
    10501 tggtaagccg gcaattaatg gcagccgtcg tctgggggtg gcactggcta ctgcagagag
    10561 tgttgttgac gccattaaac gcgcgaagca cgccgccgga caggtaaaag tacagggtta
    10621 aacccggcaa aaaaacgcta caaaaatgcc cgatccgcga tcgggcattt tgacttttac
    10681 agcttagcgc cttctacagc ttcacgcgcc agcttagtaa tacggtcgta atcgcccgct
    10741 tccagcgcat ctgccggaac cagccaggaa ccaccgatgc acagcacgct tttcagcgcc
    10801 aggtagtcac ggtagttagc cggagaaata ccacctgtcg ggcagaaacg gacctgggag
    10861 aacggacccg cgatcgcctg cagggctttc acgccgccgt tagcttcagc cgggaagaat
    10921 ttgaactctt tcaaaccgta gtccataccc agcatcagtt cggaaacagt gctgatcccc
    10981 ggaatcagag gaatagtccc ttcggtagca gctttcagca gcggctcggt cagacccggg
    11041 ctaattgcga actgtgcacc cgcttcggtg acttctgcca gctgctgtgg attcagcacc
    11101 gtaccggcac ccacaatcgc ttcaggcact tctttggcga tagcacggat agcgtcaact
    11161 gcacactcgg tacgcagagt cacttccaga acgcgcaccc caccagcaac caacgctttt
    11221 gccatcggca ctgcgtgttc cagttttttt accacgataa ccggtacaac cgggccggtg
    11281 gtcaggattg attctgcact tgttttccag tttttcatca gagttttctc tcgcctgatt
    11341 acaaatttgt cgtcttaaaa agtgatacag gttgcgccct gttcggcacc ggacagtttt
    11401 tcacgcaagg cgctgaataa ttcacgtcct gtccccacgc gtgacgcgct caggtcagga
    11461 atgtgcggtt cgcgagcagc cagttccgct tcgtctacca gcagcgtcag ttcgcctgtc
    11521 tgtccattca cacgaatgat gtccccgtcg cgcacttttg ccagcagccc gccatcgtag
    11581 gcttctggtg ttacgtggat agctgacggc actttacctg aagcgccgga gagtcgtcca
    11641 tcggtaacta acgcaatttt gaaacaccgg tccaataata caccaagtgg cggcatgagt
    11701 ttatgtaatt ctggcattcc gttcgctttt ggcccctgat gacggacaac aacgacacaa
    11761 tcgcggtcca gcaaacccgc ttcaaaggcc ggcataacgt catgctggct ttcaaaaaca
    11821 accgctggcg cttcaatcac ctggttctca accggcacgg cagaggtttt cataaccgca
    11881 cggcccaggt taccgcttaa cacttttgtc ccaccatgat gagagaaagg ttgttcgaag
    11941 gaagcgatca cattgctgtc gagtgatttt tccgcccctt cccgccagtc cagttcacca
    12001 ttattcagcc atggttcaag ggtataacga gacagaccaa agcctgccac cgtattgaca
    12061 tcttcatgca gcaggcctgc tttgagcagt tcacgcacca gaaccggtac gccacctgcc
    12121 gcctggaagt ggttaatatc ggccggaccg ttcgggtaga gacgtgccat cagcggtaca
    12181 acatcagaaa ggtcagagaa gtcatcccag ttaatctgaa taccggccgc gcgcgccatc
    12241 gccaccaggt gcatggtgtg gttagtggaa ccaccggtcg ccagcagtgc aacgataccg
    12301 ttcaccacca ctttctcatc gatcatctta ccgatcggca tccattcatt accattaccg
    12361 gtcatgcgtg taacctgacg cgcagcggca gcggtcaaag catcgcgcag cggagaatcc
    12421 ggatgaacaa aagaagagcc tggcaactgc atccccataa attccaccac catctggttg
    12481 gtgttggcag taccgtagaa agtacatgtt cccggcgcat ggtaagacgc ggcttctgac
    12541 tccagtaagg ccatgcggtc cactttacct tcggcataaa gctggcgaat acgcactttt
    12601 tctttatttg gcaaaccgct tgccatcggt ccagacggca caaacaccgc aggcaaatga
    12661 ccaaacgaca gggctgccat cgtcagaccc gggacaatct tgtcgcacac accgaggaac
    12721 agagcaccat caaacatgtt atgggacagc ccgaccgccg cagacatcgc tatcacttcg
    12781 cggcttagca gcgacaattc cattccatcc tgcccctggg tgacaccatc acacatcgcc
    12841 ggaacaccgc ccgcaacctg accaaccgca ttcgcttcat gcagggcttt acgaatgatt
    12901 tctggatagt gttcataagg ctggtgcgcg gagagcatgt cgttatagga ggtgatgatg
    12961 gcgatattgt tacgcaacat gcttttcaaa gaggctttgt cttctggctg gcaggcagcg
    13021 aaaccgtgtg ccaggttacc gcatgccaac tgcgaacgat gaacggtcga agttttcgct
    13081 tgttctatcc gggcgagata agcagagcga gtctcgcgcg aacgttcaat gattcgattt
    13141 gttacgcgta acaattgtgg attcataaag gctcctgaaa ttgagttgtc agagcaggat
    13201 gattcacaac gcgtttcatt cagaggattt atgactgaaa cgcctgtaac cggagctcat
    13261 agggcaaaaa cgtttcagtc agtgtaataa aaaaagcctc gtgggtgaat ccgcacgagg
    13321 cctgaaagtg taaaaattgt tctacaatct gtgcaagatc atgttaccgg taaaataacc
    13381 ataaaggata agcgcagata ttactcaaac tcattccagg aacgaccatc acgggtaatc
    13441 atcgccaccg aggcaacggg tccccaggtt ccggcctgat acggtttcgg cgcatcattg
    13501 tccatcgccc acgcctcagt aatggagtct acccatttcc aggcttcttc cacttcgtcg
    13561 cgacgaacaa acagtgcctg aataccacgc atggtttcca gcagcaaacg ttcataggca
    13621 tccgccagat gcgtctgatt aaaggtttct gaatagctca gatccagctt ggtgatttgc
    13681 aggttatgtt tgtggtcaag gccaggaact ttattcagta cctggatatc cacgccttca
    13741 tcaggttgca gacggatagt cagtttattc tgcggcagat cctgccacga ttctttaaac
    13801 agattcagtt caggtgtttt gaaatagacc acgacttcag aacatttggt cggcagacgt
    13861 ttaccagtac gcaggtagaa tggcacaccg gcccagcgcc agttatcaat gtcgacgcgg
    13921 atcgctacga aggtttctgt attgctgctc ttgttcgcgc cctcttcttc cagatatccc
    13981 ggcacttttt tgccctgggc gaagcccgca gtatattgcc cgcgtacggt tttttcgcgt
    14041 acgttggagc ggtcgatgcg gcgcagagac ttcagtactt tcactttttc atcgcggatg
    14101 ctgtctgcgc tcaggtcaga cggcggagac atcgcaatca tgcagagaat ttgcagcagg
    14161 tggttttgga tcatatcgcg catctgaccg gctttatcaa aatagcccca gcgcccttcg
    14221 atccccactt cttctgccac ggtaatctca acatgatcaa tggtgcgatt gtcccagtta
    14281 ttcacaaaca gggagttagc aaaacgcagc gccaacaggt tcagcaccgt ttctttacca
    14341 agatagtggt cgatacggta aacctggcac tcctcgaagt attcgccaac ctgatcattg
    14401 atttcctgcg aggtcgccag cgacgtcccc agcggtttct ccatgactac gcgtgccggt
    14461 ttagcattca gtttagcctc gccaagccct ttgcaaattg cgccaaaagt gctgggcggc
    14521 atggcaaagt agttaatggt gatacgattt ttttgatcca gcatcgcgcc gagacggctg
    14581 aatgcagcag tgtcattgac atcgagatta caaaaatcca gacgtgcact cagggtgtcc
    14641 cataaacctt catcaatggt ttctttcatg aaagtttcga gcgcctcgcg gacaactttg
    14701 gtatatgccg ctttatccca gtcagcacgc cctacgccga taatccgggt gtccgggttg
    14761 agctgaccgg ctttttccag ttgatacagg gaaggcagca atttacgacg cgcaaggtcg
    14821 cctttcgcgc cgaaaatgac caggtcacag gcctgggctg tttgcgttac cgccatgtca
    14881 ttctccttaa gttaactaac ccggtactta agccagggta tacttgtaat tttcttacgg
    14941 tgcactgtac tgcttttacg agcttgcgaa aactgtaaac gcttatccac ccgtgcgatt
    15001 acggtaaaag cgcgcaaagt gcggcaaaac tgataaaaaa atcatcgttt tccattgggt
    15061 aaaaatctga cactgatcat gttatgaaaa aaaataacaa cttttttatc tgcttttgtc
    15121 attaacacag cacacaggcg taatatctga caaaactaca tttcgatttc tttcagtgcg
    15181 gaaatcgtca ttacccgtga gtttctttac atcatgaata tgctggaaaa aatccagtct
    15241 cagctggaac atttgagcaa atcagagcgc aaagttgccg aggtcattct ggcttcgccc
    15301 gataacgcga tccattcgag tattgctgct ctggcgctgg aagccaatgt tagcgaaccg
    15361 acggtgaatc gtttctgtcg cagcatggac acgcgcggtt ttcctgattt taaacttcat
    15421 ctggcacaaa gtctggcgaa tggcactccc tatgttaatc gcaatgtcaa tgaagatgac
    15481 agcgttgaat catacacagg gaaaatattt gagtccgcaa tggcaacgct tgatcatgtc
    15541 cgtcattcac tggataaatc tgccatcaac cgcgccgtcg acttgctcac tcaggcaaaa
    15601 aaaatcgcct ttttcggatt aggctcttca gccgccgttg cccacgatgc gatgaataag
    15661 ttctttcgtt ttaatgttcc ggtggtgtac tccgatgata tcgtgctgca acgcatgagt
    15721 tgtatgaatt gtagcgacgg agacgtggtg gtgctgattt ctcacactgg aagaacaaaa
    15781 aatctggtcg agctggcgca gctggctcgc gaaaacgacg ccatggtgat tgccctcacc
    15841 tctgcgggta ccccgctcgc ccgggaagca acgctggcaa ttaccctcga cgtaccggaa
    15901 gatactgaca tttatatgcc catggtttct cgacttgcac agctgaccgt gatagatgtg
    15961 ctggcgacag gatttacttt gcgacgcggt gcaaaattca gagataactt gaagcgggtc
    16021 aaggaagcgc tgaaggaatc gcgttttgat aagcagttac ttaatttaag tgacgatcgc
    16081 taaaaacgac tgtcactgtc ctaatcttat acgacatccg aatgagatta atttatcgcc
    16141 atcgcggcgt tatttcattc ggatttcatg ttcaagcaac acctggttgt ttcagtcaac
    16201 ggagtattac atgtccagaa ggcttcgcag aacaaaaatc gttaccacgt taggcccagc
    16261 aacagatcgc gataataatc ttgaaaaagt tatcgcggcg ggtgccaacg ttgtacgtat
    16321 gaacttttct cacggctcgc ctgaagatca caaaatgcgc gcggataaag ttcgtgagat
    16381 tgccgcaaaa ctggggcgtc atgtggctat tctgggtgac ctccaggggc ccaaaatccg
    16441 tgtatccacc tttaaagaag gcaaagtttt cctcaatatt ggggataaat tcctgctcga
    16501 cgccaacctg ggtaaaggtg aaggcgacaa agaaaaagtc ggtatcgact acaaaggcct
    16561 gcctgctgac gttgtgcctg gcgacatcct gctgctggac gatggtcgcg ttcagttaaa
    16621 agtactggaa gttcagggca tgaaagtgtt caccgaagtg accgtcggtg gtcccctctc
    16681 caacaataaa ggtatcaaca aacttggcgg cggtttgtcg gctgaagcgc tgaccgaaaa
    16741 agacaaagca gacattaaga ctgcggcgtt gattggcgta gattacctgg ctgtctcctt
    16801 cccacgctgc ggcgaagatc tgaactatgc ccgtcgcctg gcacgcgatg caggatgtga
    16861 tgcgaaaatt gttgccaagg ttgaacgtgc ggaagccgtt tgcagccagg atgcaatgga
    16921 tgacatcatc ctcgcctctg acgtggtaat ggttgcacgc ggcgacctcg gtgtggaaat
    16981 tggcgacccg gaactggtcg gcattcagaa agcgttgatc cgtcgtgcgc gtcagcttaa
    17041 ccgcgcggta atcacggcga cccagatgat ggagtcaatg attactaacc cgatgccgac
    17101 gcgtgcagaa gtcatggacg tagcaaacgc cgttctggat ggtactgacg ctgtgatgct
    17161 gtctgcagaa actgccgctg ggcagtatcc gtcagaaact gttgcagcca tggcgcgcgt
    17221 ttgcctgggt gcggaaaaaa tcccgagcat caacgtttct aaacaccgtc tggacgttca
    17281 gttcgacaat gtggaagaag ctattgccat gtcagcaatg tacgcagcta accacctgaa
    17341 aggcgttacg gcgattatta ccatgaccga atcgggtcgt accgcgctga tgacctcccg
    17401 tatcagctct ggtctaccaa ttttcgccat gtcgcgccat gaacgtacgc tgaacctgac
    17461 tgctctctat cgtggcgtta cgccggtgca ctttgatagc gctaatgacg gcgtagcagc
    17521 tgccagcgaa gcggttaatc tgctgcgcga taaaggttac ttgatgtctg gtgacctggt
    17581 gattgtcacc cagggcgacg tgatgagtac cgtgggttct actaatacca cgcgtatttt
    17641 aacggtagag taatctcacc tgccccggcc gctgatgacc ggggtattta gcaacacata
    17701 aattaggact actcattcaa tccaatcata atcacagccc cctgtaaaat gaatacaggg
    17761 atagcctgaa tggagatcta aatagcttgc aagacgtcgc atacccgctt cctctgcaac
    17821 actatatcca ataactgcca tcggtaagcc actatctttg gagtattgtg tctgcaccca
    17881 tgttttaatt gaatcgttaa tcgtaatata ataatcagcc tgatattcca tatattgtat
    17941 tggatcacaa aaacagcctg ccccaatccc aatagttcta actattctgg ctctatctcc
    18001 ataaaattgg atcccaggaa gattataaat tttcaaattt ttggcaaaac gtttggtaat
    18061 attaatggca ctgtcaggtt ctatagcata tacatggtac atatcatcag aagcaacaat
    18121 atttgtaaca tccagttcta actgctgagc caaagcctta gacattccga acgtaggctc
    18181 tcgatccatg acatcatggc aacgaatgat taccaaatta tttttatgca aaaactcctt
//
LOCUS       DAZKHF020000076        17989 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2201S31241
            SAMN43267800-rid25106413.denovo.076, whole genome shotgun sequence.
ACCESSION   DAZKHF020000076 DAZKHF020000000
VERSION     DAZKHF020000076.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267800
            Sequence Read Archive: SRR30307263
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 17989)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17989)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 17989)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350581.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350581.2
            Genome Coverage       :: 151x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:21:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,652
            CDSs (total)                      :: 5,557
            Genes (coding)                    :: 5,320
            CDSs (with protein)               :: 5,320
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 237
            CDSs (without protein)            :: 237
            Pseudo Genes (ambiguous residues) :: 0 of 237
            Pseudo Genes (frameshifted)       :: 102 of 237
            Pseudo Genes (incomplete)         :: 130 of 237
            Pseudo Genes (internal stop)      :: 52 of 237
            Pseudo Genes (multiple problems)  :: 41 of 237
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17989
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267800-rid25106413.denovo.076"
                     /serotype="O 26:H11"
                     /isolate="2201S31241"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-01"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            64..1479
                     /gene="tnaA"
                     /locus_tag="ACHOSC_RS10680"
                     /old_locus_tag="pgaptmp_002136"
     CDS             64..1479
                     /gene="tnaA"
                     /locus_tag="ACHOSC_RS10680"
                     /old_locus_tag="pgaptmp_002136"
                     /EC_number="4.1.99.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709490.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009034 - tryptophanase activity [Evidence
                     IEA];
                     GO_process: GO:0006568 - tryptophan metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophanase"
                     /protein_id="HGU4548072.1"
                     /translation="MENFKHLPEPFRIRVIEPVKRTTRAYREEAIIKSGMNPFLLDSE
                     DVFIDLLTDSGTGAVTQSMQAAMMRGDEAYSGSRSYYALAESVKNIFGYQYTIPTHQG
                     RGAEQIYIPVLIKKREQEKGLDRSKMVAFSNYFFDTTQGHSQINGCTVRNVYIKEAFD
                     TGVRYDFKGNFDLEGLERGIEEVGPNNVPYIVATITSNSAGGQPVSLANLKAMYSIAK
                     KYDIPVVMDSARFAENAYFIKQREAEYKDWTIEQITRETYKYADMLAMSAKKDAMVPM
                     GGLLCMKDDSFFDVYTECRTLCVVQEGFPTYGGLEGGAMERLAVGLYDGMSLDWLAYR
                     IAQVQYLVDGLEEIGVVCQQAGGHAAFVDAGKLLPHIPADQFPAQALACELYKVAGIR
                     AVEIGSFLLGRDPKTGKQLPCPAELLRLTIPRATYTQTHMDFIIEAFKHVKENAANIK
                     GLTFTYEPKVLRHFTAKLKEV"
     gene            1570..2817
                     /gene="tnaB"
                     /locus_tag="ACHOSC_RS10685"
                     /old_locus_tag="pgaptmp_002137"
     CDS             1570..2817
                     /gene="tnaB"
                     /locus_tag="ACHOSC_RS10685"
                     /old_locus_tag="pgaptmp_002137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="low affinity tryptophan permease TnaB"
                     /protein_id="HGU4548073.1"
                     /translation="MTDQAEKKHSAFWGVMVIAGTVIGGGMFALPVDLAGAWFFWGAF
                     ILIIAWFSMLHSGLLLLEANLNYPVGSSFNTITKDLIGNTWNIISGITVAFVLYILTY
                     AYISANGAIISETISMNLGYHANPRIVGICTAIFVASVLWISSLAASRITSLFLGLKI
                     ISFVIVFGSFFFQVDYSILRDATSTTAGTSYFPYIFMALPVCLASFGFHGNIPSLIIC
                     YGKRKDKLIKSVVFGSLLALVIYLFWLYCTMGNIPRESFKAIISSGGNVDSLVKSFLG
                     TKQHGIIEFCLLVFSNLAVASSFFGVTLGLFDYLADLFKIDNSHGGRFKTVLLTFLPP
                     ALLYLIFPNGFIYGIGGAGLCATIWAVIIPAVLAIKARKKFPNQMFTVWGGNLIPAIV
                     ILFGITVILCWFGNVFNVLPKFG"
     gene            2949..4124
                     /gene="mdtL"
                     /locus_tag="ACHOSC_RS10690"
                     /old_locus_tag="pgaptmp_002138"
     CDS             2949..4124
                     /gene="mdtL"
                     /locus_tag="ACHOSC_RS10690"
                     /old_locus_tag="pgaptmp_002138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter MdtL"
                     /protein_id="HGU4548074.1"
                     /translation="MSRFLICSFALVLLYPAGIDMYLVGLPRIAADLNASEAQLHIAF
                     SVYLAGMAAAMLFAGKVADRSGRKPVAIPGAALFIIASVFCSLAETSTLFLAGRFLQG
                     LGAGCCYVVAFAILRDTLDDRRRAKVLSLLNGITCIIPVLAPVLGHLIMLKFPWQSLF
                     WTMAMMGIAVLMLSLFILKETRPASPAASDKPRENSESLLNRFFLSRVVITTLSVSVI
                     LTFVNTSPVLLMEIMGFERGEYATIMALTAGVSMTVSFSTPFALGIFKPRTLMITSQV
                     LFLAAGITLAVSPSHAVSLFGITLICAGFSVGFGVAMSQALGPFSLRAGVASSTLGIA
                     QVCGSSLWIWLAAVVGIGAWNMLIGILIACSIVSLLLIMFVAPGRPVAAHEEIHHHA"
     gene            4099..5058
                     /gene="yidZ"
                     /locus_tag="ACHOSC_RS10695"
                     /old_locus_tag="pgaptmp_002139"
     CDS             4099..5058
                     /gene="yidZ"
                     /locus_tag="ACHOSC_RS10695"
                     /old_locus_tag="pgaptmp_002139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator YidZ"
                     /protein_id="HGU4548075.1"
                     /translation="MKKSITTLDLNLLLCLQLLMQERSVTKAAKRMNVTPSAVSKSLA
                     KLRAWFDDPLFVNSPLGLSPTPLMVSMEQNLAEWMQMSNLLLDKPHHQTPRGLKFELA
                     AESPLMMIMLNALSKRIYQRYPQATIKLRNWDYDSLDAITRGEVDIGFSGRESHPRSR
                     ELLSSLPLAIDYEVLFSDVPCVWLRKDHPALHETWNLDTFLRYPHISICWEQSDTWAL
                     DNVLQELGRERTIAMSLPEFEQSLFMAAQPDNLLLATAPRYCQYYNQLHQLPLVALPL
                     PFDESQQKKLEVPFTLLWHKRNSHNPKIVWLRETIKNLYASMA"
     gene            5215..5964
                     /locus_tag="ACHOSC_RS10700"
                     /old_locus_tag="pgaptmp_002140"
     CDS             5215..5964
                     /locus_tag="ACHOSC_RS10700"
                     /old_locus_tag="pgaptmp_002140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709485.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4'-phosphopantetheinyl transferase superfamily
                     protein"
                     /protein_id="HGU4548076.1"
                     /translation="MATHFARGILTEGHLISVRLPSQCHQEARNIPPHRQSRFLASRG
                     LLAELMFMLYGIGELPEIVTLPKGKPVFSDKNLPSFSISYAGNMVGVALTTEGECGLD
                     MELQRATRGFHSPHAPDNHTFSSNESLWISKQNDPNEARAQLITLRRSVLKLTGDVLN
                     DDPRDLQLLPIAGRLKCAHVNHVEALCDAEDVLVWSVAVTPAIEKLSVWELDGKHSWK
                     SLPDIHSRANNPTSRMMRFAQLSTVKSFSPN"
     gene            5986..6552
                     /gene="yieF"
                     /locus_tag="ACHOSC_RS10705"
                     /old_locus_tag="pgaptmp_002141"
                     /gene_synonym="chrR"
     CDS             5986..6552
                     /gene="yieF"
                     /locus_tag="ACHOSC_RS10705"
                     /old_locus_tag="pgaptmp_002141"
                     /gene_synonym="chrR"
                     /EC_number="1.6.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I chromate reductase YieF"
                     /protein_id="HGU4548077.1"
                     /translation="MSEKLQVVTLLGSLRKGSFNGMVARTLPKIAPASMEVNALPSIA
                     DIPLYDADVQQEEGFPATVEALAEQIRQADGVVIVTPEYNYSVPGGLKNAIDWLSRLP
                     DQPLAGKPVLIQTSSMGVIGGARCQYHLRQILVFLDAMVMNKPEFMGGVIQNKVDPQT
                     GEVIDQGTLDHLTGQLTAFGEFIQRVKI"
     gene            complement(6606..7943)
                     /gene="adeP"
                     /locus_tag="ACHOSC_RS10710"
                     /old_locus_tag="pgaptmp_002142"
     CDS             complement(6606..7943)
                     /gene="adeP"
                     /locus_tag="ACHOSC_RS10710"
                     /old_locus_tag="pgaptmp_002142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenine permease AdeP"
                     /protein_id="HGU4548078.1"
                     /translation="MSQQHTTQASGQGMLERVFKLREHGTTARTEVIAGFTTFLTMVY
                     IVFVNPQILGVAGMDTSAVFVTTCLIAAFGSIMMGLFANLPVALAPAMGLNAFFAFVV
                     VQAMGLPWQVGMGAIFWGAIGLLLLTIFRVRYWMIANIPVSLRVGITSGIGLFIGMMG
                     LKNAGVIVANPETLVSIGNLTSHSVLLGILGFFIIAILASRNIHAAVLVSIVVTTLLG
                     WMLGDVHYNGIVSAPPSVMTVVGHVDLAGSFNLGLAGVIFSFMLVNLFDSSGTLIGVT
                     DKAGLADEKGKFPRMKQALYVDSISSVTGSFIGTSSVTAYIESSSGVSVGGRTGLTAV
                     VVGLLFLLVIFLSPLAGMVPGYAAAGALIYVGVLMTSSLARVNWQDLTESVPAFITAV
                     MMPFSFSITEGIALGFISYCVMKIGTGRLRDLSPCVIIVALLFILKIVFIDAH"
     gene            8110..8775
                     /gene="yieH"
                     /locus_tag="ACHOSC_RS10715"
                     /old_locus_tag="pgaptmp_002143"
     CDS             8110..8775
                     /gene="yieH"
                     /locus_tag="ACHOSC_RS10715"
                     /old_locus_tag="pgaptmp_002143"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphogluconate phosphatase"
                     /protein_id="HGU4548079.1"
                     /translation="MSQIEAVFFDCDGTLVDSEVICSRAYVTMFQEFGITLDPEEVFK
                     RFKGVKLYEIIDIVSLEHGVTLAKTEAEHVYRAEVARLFDSELEAIEGAGALLSAITA
                     PMCVVSNGPNNKMQHSMGKLNMLHYFPDKLFSGYDIQRWKPDPALMFHAAKAMNVNVE
                     NCILVDDSVAGAQSGIDAGMEVFYFCADPHNKPIVHPKVTTFTHLSQLPELWKARGWD
                     ITA"
     gene            8842..9315
                     /locus_tag="ACHOSC_RS10720"
                     /old_locus_tag="pgaptmp_002144"
     CDS             8842..9315
                     /locus_tag="ACHOSC_RS10720"
                     /old_locus_tag="pgaptmp_002144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4548080.1"
                     /translation="MSVSRRVIHHGLYFAVLGPLIGVLFLVLYIFFAKEPLVLLRRIQ
                     VLPLFLLLSITTGVIPALLTGVMVACLPEKIGSQKNYRCLAGGIGGVVITEIYCAVIV
                     HIKGMASSELYENILSGDSLVVRIIPALLAGVVMSRIITRLPGLDISCPETDSLS"
     gene            9364..9951
                     /gene="cbrC"
                     /locus_tag="ACHOSC_RS10725"
                     /old_locus_tag="pgaptmp_002145"
     CDS             9364..9951
                     /gene="cbrC"
                     /locus_tag="ACHOSC_RS10725"
                     /old_locus_tag="pgaptmp_002145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0030153 - bacteriocin immunity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PF03691 family colicin E2 tolerance protein
                     CbrC"
                     /protein_id="HGU4548081.1"
                     /translation="MTQNIRPLPQFKYHPKPLETGAFEQDKTVECDCCEQQTSVYYSG
                     PFYCVDEVEHLCPWCIADGSAAEKFAGSFQDDASIEGVEFEYDEEDEFAGIKNTYPDE
                     MLKELVERTPGYHGWQQEFWLAHCGDFCAFIGYVGWNDIKDRLDEFANLEEDCENFGI
                     RNSDLAKCLQKGGDCQGYLFRCLHCGKLRLWGDFS"
     gene            complement(10013..10735)
                     /locus_tag="ACHOSC_RS10730"
                     /old_locus_tag="pgaptmp_002146"
     CDS             complement(10013..10735)
                     /locus_tag="ACHOSC_RS10730"
                     /old_locus_tag="pgaptmp_002146"
                     /EC_number="3.5.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000766899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004342 - glucosamine-6-phosphate
                     deaminase activity [Evidence IEA];
                     GO_process: GO:0006044 - N-acetylglucosamine metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucosamine-6-phosphate deaminase"
                     /protein_id="HGU4548082.1"
                     /translation="MKLIITEDYQEMSRVAAHHLLGYMSKTRRVNLAITAGSTPKGMY
                     EYLTTLVKGKPWYDNCYFYNFDEIPFRGKEGEGVTITNLRNLFFTPAGIKEENIQKLT
                     IDNYREHDQKLAREGGLDLVVLGLGADGHFCGNLPNTTHFHEQTVEFPIQGEMVDIVA
                     HGELGGDFSLVPDSYVTMGPKSIMAAKNLLIIVSGAGKAQALKNVLQGPVTEDVPASV
                     LQLHPSLMVIADKAAAAELALD"
     gene            complement(10750..11919)
                     /locus_tag="ACHOSC_RS10735"
                     /old_locus_tag="pgaptmp_002147"
     CDS             complement(10750..11919)
                     /locus_tag="ACHOSC_RS10735"
                     /old_locus_tag="pgaptmp_002147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418175.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase"
                     /protein_id="HGU4548083.1"
                     /translation="MNIKIAALTLAIASGISAQWAIAADMPASPAPTIPVKQYVTQVN
                     ADNSVTFRYFAPGAKNVSVVVGVPVPDNIHPMTKDEAGVWSWRTPILKGNLYEYFFNV
                     DGVRSIDTGTAMTKPQRQVNSSMILVPGSYLDTRSVAHGDLIAITYHSNALQSERQMY
                     VWTPPGYTGMGEPLPVLYFYHGFGDTGRSAIDQGRIPQIMDNLLAEGKIKPMLVVIPD
                     TETDAKGIIPEDFVPQERRKVFYPLNAKAADRELMNDIIPLISKRFNVRKDADGRALA
                     GLSQGGYQALVSGMNHLESFGWLATFSGVTTTTVPDEGVAARLNDPAAINQQLRNFTV
                     VVGDKDVVTGKDIAGLKTELEQKKIKFDYQEYPGLNHEMDVWRPAYAAFVQKLFK"
     gene            complement(11946..13562)
                     /gene="bglH"
                     /locus_tag="ACHOSC_RS10740"
                     /old_locus_tag="pgaptmp_002148"
     CDS             complement(11946..13562)
                     /gene="bglH"
                     /locus_tag="ACHOSC_RS10740"
                     /old_locus_tag="pgaptmp_002148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate-specific porin BglH"
                     /protein_id="HGU4548084.1"
                     /translation="MFRRNLITSAILLMAPLAFSAQSLAESLTVEHRLELLEKALRET
                     QSELKKYKDEEKKKYTPATVNRSVSTNDQGYAANPFPTSSAAKPDAVLVKNEEKNASE
                     TGSIYSSMTLKDFSKFVKDEIGFSYNGYYRSGWGTASHGSPKSWAIGSLGRFGNEYSG
                     WFDLQLKQRVYNENGKRVDAVVMMDGNVGQQYSTGWFGDNAGGENFMQFSDMYVTTKG
                     FLPFAPEADFWVGKHGAPKIEIQMLDWKTQRTDAAAGVGLENWKVGPGKIDIALVRED
                     VDDYDRSLQNKQQINTNTIDLRYKDIPLWDKATLMVSGRYVTANESASEKDNQDNNGY
                     YDWKDTWMFGTSLTQKFDKGGFNEFSFLVANNSIASNFGRYAGASPFTTFNGRYYGDH
                     TGGTAVRLTSQGEAYIGDHFIVANAIVYSFGNDIYSYETGAHSDFESIRAVVRPAYIW
                     DQYNQTGVELGYFTQQNKDANSNKFNESGYKTTLFHTFKVNTSMLTSRPEIRFYATYI
                     KALENELDGFTFEDNKDDQFAVGAQAEIWW"
     gene            complement(13648..15042)
                     /gene="bglB"
                     /locus_tag="ACHOSC_RS10745"
                     /old_locus_tag="pgaptmp_002149"
     CDS             complement(13648..15042)
                     /gene="bglB"
                     /locus_tag="ACHOSC_RS10745"
                     /old_locus_tag="pgaptmp_002149"
                     /EC_number="3.2.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001469563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase BglB"
                     /protein_id="HGU4548085.1"
                     /translation="MKAFPETFLWGGATAANQVEGAWQEDGKGISTSDLQPHGVMGKM
                     EPRILGKENIKDVAIDFYHRYPEDIALFAEMGFTCLRISIAWARIFPQGDEVEPNEAG
                     LAFYDRLFDEMAQAGIKPLVTLSHYEMPYGLVKNYGGWANRAVIDHFEHYARTVFTRY
                     QHKVALWLTFNEINMSLHEPFTGVGLAEESGEAEVYQAIHHQLVASARAVKACHSLLP
                     EAKIGNMLLGGLVYPLTCQPQDMLQAMEENRRWMFFGDVQARGQYPGYMQRFFRDHNI
                     TIEMTESDAEDLKHTVDFISFSYYMTGCVSHDESINKNAQGNILNMIPNPHLKSSEWG
                     WQIDSVGLRVLLNTLWDRYQKPLFIVENGLGAKDSVEADGSIQDDYRIAYLNDHLVQV
                     NEAIADGVDIMGYTSWGPIDLVSASHSQMSKRYGFIYVDRDDNGEGSLTRTRKKSFGW
                     YAEVIKTRGLSLKK"
     gene            complement(15061..16938)
                     /gene="bglF"
                     /locus_tag="ACHOSC_RS10750"
                     /old_locus_tag="pgaptmp_002150"
     CDS             complement(15061..16938)
                     /gene="bglF"
                     /locus_tag="ACHOSC_RS10750"
                     /old_locus_tag="pgaptmp_002150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS beta-glucoside transporter subunit IIABC"
                     /protein_id="HGU4548086.1"
                     /translation="MTELARKIVAGVGGADNIVSLMHCATRLRFKLKDESKAQAEVLK
                     KTPGIIMVVESGGQFQVVIGNHVADVFLAVNSVAGLGEKAQQAPENDEKGNLLNRFVY
                     VISGIFTPLIGLMAATGILKGMLALALTFQWTTEQSGTYLILFSASDALFWFFPIILG
                     YTAGKRFGGNPFTAMVIGGALVHPLILTAFENGQKADELGLDFLGIPVTLLNYSSSVI
                     PIIFSAWLCSILERRLNAWLPSAIKNFFTPLLCLMVITPVTFLLVGPLSTWISELIAA
                     GYLWLYQAVPAFAGAVMGGFWQIFVMFGLHWGLVPLCINNFTVLGYDTMIPLLMPAIM
                     AQVGAALGVFLCERDAQKKVVAGSAALTGLFGITEPAVYGVNLPRKYPFVIACISGAL
                     GATIIGYAQTKVYSFGLPSIFTFMQTIPSTGIDFTVWASVIGGVIAIGCAFVGTVMLH
                     FITAKRQPAQGAPQEKTPEVITPPEQGGICSPMTGEIVPLIHVADTTFASGLLGKGIA
                     ILPSVGEVRSPVAGRIASLFATLHAIGIESDDGVELLIHVGIDTVKLDGKFFSAHVNV
                     GDKVNTGDRLISFDIPAIREAGFDLTTPVLISNSDDFTDVLPHGTAQISAGEPLLSII
                     R"
     gene            complement(17072..17908)
                     /gene="bglG"
                     /locus_tag="ACHOSC_RS10755"
                     /old_locus_tag="pgaptmp_002151"
     CDS             complement(17072..17908)
                     /gene="bglG"
                     /locus_tag="ACHOSC_RS10755"
                     /old_locus_tag="pgaptmp_002151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional antiterminator BglG"
                     /protein_id="HGU4548087.1"
                     /translation="MNMQITKILNNNVVVVIDDQQREKVVMGRGIGFQKRAGERINSS
                     GIEKEYALSSHELNGRLSELLSHIPLEVMATCDRIISLAQERLGKLQDSIYISLTDHC
                     QFAIKRFQQNVLLPNPLLWDIQRLYPKEFQLGEEALTIIDKRLGVQLPKDEVGFIAMH
                     LVSAQMSGNMEDVAGVTQLMREMLQLIKFQFSLNYQEESLSYQRLVTHLKFLSWRILE
                     HASINDSDESLQQAVKQNYPQAWQCAERIAIFIGLQYQRKISPAEIMFLAINIERVRK
                     EH"
BASE COUNT         4529 a         4508 c         4267 g         4685 t
ORIGIN      
        1 tggatattgc agatgtaata ttcacaggga tcactgtaat taaaataaat gaaggattat
       61 gtaatggaaa actttaaaca tctccctgaa ccgttccgca ttcgtgttat tgagccagta
      121 aaacgtacca ctcgcgctta tcgtgaagag gcaattatta aatccggtat gaacccattc
      181 ctgctggata gcgaagatgt gtttatcgat ttactgaccg acagcggcac cggggcggtg
      241 acgcagagta tgcaggccgc gatgatgcgc ggcgacgaag cctacagcgg cagccgcagc
      301 tactatgcgt tagccgagtc agtgaaaaat atctttggtt atcaatacac cattccgact
      361 caccagggcc gtggcgcaga gcaaatttat attccggtac tgattaaaaa acgcgagcag
      421 gaaaaaggcc tggatcgcag caaaatggtg gcattctcta actatttctt tgataccacg
      481 cagggccata gccagattaa cggctgtacc gtgcgtaacg tctatatcaa agaagccttc
      541 gatactggcg tgcgttacga ctttaaaggt aactttgacc ttgagggatt agaacgcggt
      601 attgaagaag ttggcccgaa taacgtgccg tatatcgttg caaccatcac cagtaactct
      661 gcaggtggtc agccggtttc actggcaaac ttaaaagcga tgtacagcat cgcgaagaaa
      721 tacgatattc cggtggtaat ggactccgca cgctttgctg aaaacgccta tttcatcaag
      781 cagcgtgaag cagaatacaa agactggacc atcgagcaga tcacccgcga aacctacaaa
      841 tatgccgata tgctggcgat gtccgccaag aaagatgcga tggtaccgat gggcggcttg
      901 ctgtgcatga aagacgacag cttctttgat gtgtacaccg agtgcagaac cctttgcgtg
      961 gtgcaggaag ggttcccgac atatggcggc ctggaaggcg gcgcgatgga gcgtctggcg
     1021 gtaggtctgt atgacggcat gagtctcgac tggctggctt atcgtatcgc gcaggtacag
     1081 tatctggtcg atggtctgga agagattggc gttgtctgcc agcaggcggg cggtcacgcg
     1141 gcattcgttg atgccggtaa actgctgccg catatcccgg cagaccagtt cccggcacag
     1201 gcgctggcgt gcgagctgta taaagtcgcc ggtatccgtg cggtagaaat tggctctttc
     1261 ctgttaggcc gcgatccgaa aaccggtaaa caactgccat gcccggctga actgctgcgt
     1321 ttaaccattc cgcgcgcaac atatactcaa acacatatgg acttcattat tgaagccttt
     1381 aaacatgtga aagagaacgc ggcgaatatt aaaggattaa cctttaccta cgaaccaaaa
     1441 gtattgcgtc acttcaccgc aaaactgaaa gaagtttaat taatactaca gagtggctat
     1501 aaggatgtta gccactctct taccctacat cctcaataac aaaaatagcc ttcctctaaa
     1561 ggtggcatca tgactgatca agctgaaaaa aagcactctg cattttgggg tgttatggtt
     1621 atagcaggta cagtaattgg tggaggtatg tttgctttac ctgttgatct tgccggtgcc
     1681 tggtttttct ggggtgcctt tatccttatc attgcctggt tttcaatgct tcattccggg
     1741 ttattgttat tagaagcaaa tttaaattat cctgtcggct ccagttttaa caccatcacc
     1801 aaagatttaa tcggtaacac ctggaacatt atcagcggta ttaccgttgc cttcgttctc
     1861 tatatcctca cttatgccta tatctctgct aatggtgcga tcattagtga aacgatatca
     1921 atgaatttgg gctatcacgc taatccacgt attgtcggga tctgcacagc cattttcgtt
     1981 gccagcgtat tgtggataag ttcgttagcc gccagtcgca ttacctcatt gttcctcggg
     2041 ctgaagatta tctcctttgt gatcgtgttt ggttctttct tcttccaggt cgattactcc
     2101 atcctgcgcg atgccaccag caccactgcg ggaacgtctt acttcccgta tatctttatg
     2161 gctttgccgg tgtgtctggc gtcatttggt ttccacggca atattcccag cctgattatt
     2221 tgctatggaa aacgcaaaga taagttaatc aaaagcgtgg tatttggttc gctgctggcg
     2281 ctggtgattt atctcttctg gctctattgc acgatgggga atattccgcg cgaaagcttt
     2341 aaggcgataa tctcctcagg cggcaacgtt gattcgctgg tgaaatcgtt cctcggcacc
     2401 aaacagcacg gcattatcga gttttgcctg ctggtgttct ctaacttagc tgttgccagt
     2461 tcgttctttg gtgtcacgct ggggttgttc gattatctgg cggacctgtt taagattgat
     2521 aactcccacg gcgggcgttt caaaaccgtg ctgttaacct tcctgccacc tgcgttgttg
     2581 tatctgatct tcccgaacgg ctttatttac gggatcggcg gtgccgggct gtgcgccacc
     2641 atctgggcgg tcattattcc cgcagtgctt gcaatcaaag ctcgcaagaa gtttcccaat
     2701 cagatgttca cggtctgggg cggcaatctt attccggcga ttgtcattct ctttggtata
     2761 accgtgattt tgtgctggtt cggcaacgtc tttaacgtgt tacctaaatt tggctaaatc
     2821 cttcaagaag ccagccattc gctggcttct tgcctctcag gaaatcactt atgtccaaat
     2881 ggcaactcgc ctgattctcc ttcaccacgt atgctttgcg tcaccttact atcaggacgc
     2941 tttagcccat gtcccgcttt ttaatttgta gttttgccct ggttttactt tatcccgccg
     3001 ggattgatat gtacctcgtt ggtttaccgc gcatcgccgc cgatctcaat gccagcgaag
     3061 cgcagttgca tattgcgttc tccgtttatc tggcgggcat ggcagctgcg atgttatttg
     3121 ccggtaaagt ggccgatcgt tcagggagaa agcctgtcgc catccccggc gcggcgctat
     3181 ttattatcgc ttcggtgttc tgttcactgg ctgaaaccag cacgttattt cttgcagggc
     3241 gctttctaca ggggttgggc gcaggctgtt gttacgtggt ggcgttcgcc attttgcgcg
     3301 acacgctgga tgatcgacgt cgggctaaag tgctgtcgtt actcaacggt attacctgca
     3361 tcattccggt gttagcgccg gtgctcggac acctgattat gcttaaattc ccttggcaga
     3421 gtctgttctg gacgatggca atgatgggca tcgcggtact gatgttatct ttgtttattt
     3481 taaaagaaac gcgcccagcg tcccccgctg cttcggacaa accacgagaa aatagcgagt
     3541 cgctgcttaa tcggtttttc ctcagccgtg ttgttatcac caccctcagc gtttcggtga
     3601 tcctcacttt cgtcaataca tcgccggtat tgctgatgga aatcatgggt tttgagcgcg
     3661 gagaatacgc caccattatg gcgttgaccg ctggcgtcag catgaccgtt tcattctcca
     3721 cgccatttgc gctgggaatt tttaagccac gtacgttgat gatcacctcg caggtgttat
     3781 tccttgcggc ggggatcacc cttgccgttt caccttccca tgcggtttct ctgtttggta
     3841 tcacgctgat ttgcgccggt ttctcggtag gttttggcgt agcgatgagt caggcgttag
     3901 gaccattttc attacgcgcg ggcgtagcca gctcgacctt aggtattgcg caggtttgcg
     3961 gttcgtcact gtggatttgg ctggcagcgg tggttggtat cggcgcatgg aatatgctga
     4021 tcgggattct gattgcctgt agcatagtga gcctgttact gattatgttc gtcgcgcctg
     4081 gacgccccgt tgccgctcat gaagaaatcc atcaccacgc ttgatctcaa tctgctgctc
     4141 tgtctgcaac tgctgatgca ggagcgcagc gtgaccaaag cggcgaagcg gatgaacgtg
     4201 accccttcag cggtgagtaa gtcgctggca aagttaagag cgtggtttga cgacccgctc
     4261 tttgtgaact caccgctggg gctgtcgccc acaccgctaa tggtcagcat ggagcaaaat
     4321 ctggcggaat ggatgcaaat gagcaatctg ctgctggata aaccgcacca ccagacaccg
     4381 cgcggcctga agtttgagtt ggcggcggaa tcgccgctga tgatgatcat gcttaatgcg
     4441 ctgtcgaaac ggatctacca acgttacccg caggcgacca tcaaactacg taactgggat
     4501 tacgattcct tagatgccat tactcgtggt gaagtggata ttggtttttc cggtcgcgaa
     4561 agccatcctc gctcgcggga gctgttaagc tcgctaccgt tagccattga ttatgaagtg
     4621 ctgtttagtg atgtaccctg cgtctggtta cgcaaagatc atccggcact gcatgaaacg
     4681 tggaatctgg acaccttttt acgttatccg catatcagca tttgctggga acagagcgac
     4741 acctgggcgc tggacaatgt gttacaggag ctggggcgcg aacgcacgat tgctatgagc
     4801 ctgccggaat ttgagcagtc actgtttatg gcagcgcaac ccgacaatct gctactggcg
     4861 accgcgccgc gctactgtca gtactacaat caactccatc aactgccgtt ggttgctctt
     4921 cctctcccgt ttgacgaaag ccagcaaaaa aagctggaag ttccttttac cctgctgtgg
     4981 cataaacgga acagccataa tccgaagatc gtctggttac gggaaaccat taaaaacctt
     5041 tacgcgtcga tggcataacc gaatcgtatg aaacgcgacc caatttcaca ataaaatgta
     5101 aaaaagttgt aataagcttg tctgaatcga acttttagcc gctttagtct gtccatcatt
     5161 ccagtaaatg attactctta tattcataat ggaccattaa gcatggagcg aaaaatggcg
     5221 actcactttg cccgagggat tttaacggaa ggacatctga tttctgttcg tctcccctcc
     5281 cagtgtcatc aagaagcccg aaacattccc cctcatcgtc aaagccgttt tctggcgtcc
     5341 agaggtttac tcgcagaact gatgttcatg ctgtatggca ttggcgaatt gccggaaatc
     5401 gtcaccctgc cgaaaggtaa accggttttc agtgataaaa atttgccttc gttttctatt
     5461 tcctatgccg ggaatatggt tggcgtggcg ttaacaaccg aaggtgaatg tggcctcgat
     5521 atggaactac agcgtgcgac gcgcgggttt catagcccac acgcgcccga taaccacacc
     5581 ttttccagca atgaatcgct atggatcagt aaacaaaacg atcctaacga agcgcgggcg
     5641 cagctcatca cgctgcgccg aagcgtgcta aaactaaccg gtgatgtttt gaatgacgat
     5701 ccgcgcgatc tgcagctgct gcccattgcc ggacgcctga aatgtgctca tgtaaatcat
     5761 gtagaagcgt tatgcgacgc ggaggacgtg ctagtgtggt ccgtggcggt cacgcccgcg
     5821 attgaaaagc tcagtgtctg ggagttagat ggcaaacaca gctggaaaag cctgccggat
     5881 attcacagcc gcgccaacaa tcctaccagc cggatgatgc gttttgccca gctctctacc
     5941 gtgaagtctt tttcgccaaa ttgatagaca accacaggag tcattatgtc tgaaaaattg
     6001 caggtggtta cgttactggg gagcctgcgc aaaggctcat ttaatggcat ggttgcacgt
     6061 accctgccga aaattgctcc ggcgagcatg gaagtcaatg cgttaccttc cattgccgac
     6121 attcccctgt atgacgctga cgtacagcag gaagaaggtt ttccagcaac ggttgaagct
     6181 ctggcggaac agatccgtca ggctgacggt gtggtgatcg tcacgccgga atataactac
     6241 tcggtaccgg gtgggctgaa aaatgccatc gactggcttt cccgcctgcc ggatcaaccg
     6301 ctggccggta aaccggtgtt gattcagacc agctcaatgg gcgtgattgg cggcgcgcgc
     6361 tgtcagtatc acctgcgcca gattctggtt ttcctcgatg caatggtgat gaacaagccg
     6421 gaatttatgg gcggcgtgat tcagaacaaa gttgatccgc aaaccggaga agtgattgat
     6481 cagggtacgc tggaccacct gaccgggcaa ttgaccgcat ttggtgagtt tattcagcga
     6541 gttaagatct aaataaaaaa cccgccagca atcatgcatg gcgggttttt aacgcgctat
     6601 cgattttagt gagcgtcgat aaagacaatc ttcaggataa acagcagcgc aacgatgatt
     6661 acgcacgggc ttagatcacg cagacgtccg gtaccgattt tcatcacgca gtaggagata
     6721 aagcccagcg cgataccttc ggtaatcgag aagctgaacg gcatcatcac ggcggtaata
     6781 aacgccggaa cagattcagt aagatcctgc cagttcacgc gtgccagact ggaagtcatc
     6841 agcacgccaa cgtaaatcag cgcaccagct gcagcgtagc ctggcaccat ccccgccagc
     6901 ggcgacagaa agataaccag caggaacagc agaccaacaa ccactgccgt cagaccggta
     6961 cgaccgccaa ccgatacgcc ggaagaggac tcaatataag ccgtaacgga agaagtaccg
     7021 ataaacgaac cggtcacgga agagatactg tcgacataca gcgcctgctt catgcgcggg
     7081 aatttaccct tctcatccgc caggcctgct ttatcggtca cgccaatcag cgtaccggag
     7141 gagtcaaaca ggttgaccag catgaaagag aaaatcaccc ctgccagccc gaggttaaac
     7201 gacccggcta aatctacatg acccacaact gtcattacgc tcggcggcgc agaaacgatg
     7261 ccattgtagt gcacatcacc cagcatccag cccagcagcg tcgtcaccac gatagaaacc
     7321 agcaccgctg cgtgaatgtt gcgcgaggcc agaatagcaa tgatgaagaa gccgaggata
     7381 cccagaagta cgctgtgaga agtcagatta ccgatgctca ccagcgtttc cgggttagcg
     7441 acaatcacac ctgcgttttt cagccccatc atgccaatga acagaccgat accgctggta
     7501 atacccacac gcagactcac cggaatgttg gcaatcatcc agtagcgaac gcggaaaatc
     7561 gtcagtaaca gcagacctat cgcgccccag aagattgcgc ccatcccgac ctgccacggc
     7621 aagcccatcg cctgtacaac gacaaaagcg aagaacgcat tcaggcccat agcgggtgcc
     7681 agtgcaactg gcaggttagc aaacagtccc atcataatac tgccgaatgc agcgatcaga
     7741 caggtagtga cgaagacggc gctggtatcc atgccagcaa cgccaagaat ttgcgggtta
     7801 acaaaaacga tgtaaaccat cgtcaggaag gtggtaaaac cggcgatcac ttcggtccgt
     7861 gccgtcgtgc catgttcgcg cagtttaaac acgcgttcca gcatcccctg gccagaagcc
     7921 tgggttgtgt gttgttgact cattatctat ttccgaacaa ggagggaaaa tccgtcgcta
     7981 tcgtatacca aaatgagaca atatggacgg ctgaagataa ctttttttca ttgtatttgc
     8041 ttatacggca acgattgcgt catgcgaatt ggcatgacga aaacgttaaa ctaacgataa
     8101 aggaaagaca tgtcccagat agaagcggta tttttcgact gcgacggtac gctggtcgac
     8161 agtgaagtca tttgctcccg cgcatatgta acgatgtttc aggaatttgg tattacgctc
     8221 gatcctgaag aggtattcaa acgtttcaaa ggtgtaaaac tgtacgaaat tatcgatatt
     8281 gtttcccttg aacatggtgt tacgttagcg aaaacagaag ctgaacacgt ttaccgtgca
     8341 gaagtcgctc ggctgttcga ttcagaactg gaagccatcg aaggggctgg agcgctcctg
     8401 tcagcgatca ctgcgccaat gtgtgtggta tctaacggcc caaataacaa aatgcagcat
     8461 tctatgggca agctgaatat gttgcactac ttcccggata aactgttcag cggctacgat
     8521 attcagcgct ggaagccaga cccggcgtta atgttccatg cggcaaaagc gatgaatgta
     8581 aatgtagaaa actgcattct ggttgatgac tcagttgccg gtgcgcaatc tggtatcgac
     8641 gcaggtatgg aagtgttcta cttctgcgcc gacccgcaca ataagccgat cgttcacccg
     8701 aaagtcacca cctttaccca tctttcgcag ttaccggaac tgtggaaagc gcgtggttgg
     8761 gatattacgg catagttctt cacactccct tcacttaccc cgcttaaatt ggcgctcaaa
     8821 ggtaagtaaa gggagtttga tatgtctgtt tcacgtcggg taatacatca cggactttat
     8881 tttgcggttt taggaccgtt aattggtgtt ctgtttcttg tcctctacat attcttcgca
     8941 aaagaaccgc tggttctttt gcgaagaata caggtacttc cattgtttct cttattgtcg
     9001 attactacgg gagttattcc tgcattgtta accggcgtga tggtcgcctg tctgccggaa
     9061 aagatcgggt cacagaaaaa ttatcgttgt ctggctggtg gcataggtgg cgtcgttatc
     9121 accgagatct attgtgcagt tattgtacat attaagggca tggcttcctc ggagttgtat
     9181 gaaaacattc tttctggtga cagtctcgtt gtccgcatca ttcctgcatt gctggcaggt
     9241 gtggtgatga gcagaatcat tacccgtctg cccggattgg atatttcatg tcctgaaaca
     9301 gactctttaa gttaagcggg atactttatc tttgggctac tcaaaagcag acaggatgtt
     9361 actatgactc aaaatatcag gccgttaccc caattcaaat atcatcccaa gccactggaa
     9421 acaggcgcat ttgaacagga taaaaccgta gagtgcgatt gctgtgaaca acagacgtca
     9481 gtttattact cgggtccctt ttattgcgtt gatgaagttg aacatctctg tccgtggtgt
     9541 attgcggacg gttctgctgc tgaaaagttt gcaggtagtt ttcaggatga tgccagcata
     9601 gaaggtgttg aatttgagta tgatgaagag gacgaatttg ccggtattaa gaacacatat
     9661 cctgatgaaa tgctgaaaga gttggttgaa cgcacgccag gttatcatgg atggcaacag
     9721 gaattctggc ttgcgcattg tggcgatttc tgtgctttta tcggctatgt gggctggaat
     9781 gatataaaag atcgcctcga tgaatttgcc aaccttgaag aagattgtga gaatttcggt
     9841 attagaaatt ctgatctagc taaatgcctg caaaagggtg gtgattgtca gggttatctc
     9901 ttccgctgtc tccactgcgg caagctgaga ctgtggggtg atttttcgta gttatttaaa
     9961 taatgagaac aggccggagc gtaattcata catccggcct tatttcttca gcttaatcga
    10021 gcgctaattc tgcggcagcg gctttatcag cgattaccat cagcgaagga tgcagctgca
    10081 acacggaagc tggaacatct tcggtaaccg gaccttgcag cacatttttc agtgcctgcg
    10141 ctttacctgc gccactgaca attatcagca agtttttcgc cgccatgatg cttttcggcc
    10201 ccatcgtcac gtagctgtca ggcactaacg aaaagtcgcc gcccaactca ccatgcgcca
    10261 caatatcaac catctctccc tggatcggga actccacggt ttgctcatgg aaatgggtcg
    10321 tattcggcaa attgccacaa aaatgaccat ctgcacctaa tcccagcacc accaaatcca
    10381 ggccgccttc acgcgccagt ttctgatcat gctcgcggta gttatcaata gtgagcttct
    10441 ggatattctc ttctttgatc cccgcagggg tgaaaaacag attacgcaga ttggtaatcg
    10501 ttacaccttc tccctctttg ccgcgaaagg gaatttcatc gaaattatag aaatagcagt
    10561 tatcatacca gggcttacct ttaaccaggg tggtgaggta ttcatacatg cctttgggcg
    10621 tgctaccggc ggtaattgcc aggttaacac gacgcgtctt cgacatataa ccaagcagat
    10681 ggtgtgccgc gacacggctc atttcctggt aatcttcggt aatgattaat ttcatcttag
    10741 tgccttattt tatttaaata atttctgtac gaaggctgca taggcaggcc gccagacatc
    10801 catttcatgg ttcaggccgg gatattcctg gtaatcaaac ttaatttttt tctgctcaag
    10861 ctcagttttc agcccggcga tatccttgcc ggttacgaca tctttatcac caacgaccac
    10921 agtaaaatta cgtagttgct ggttaatagc tgccggatcg ttcagccggg ccgcgacacc
    10981 ttcatccggt acggttgtcg tggtaacacc actgaatgtg gccagccagc caaagctttc
    11041 cagatgattc attccggaaa ccagcgcctg gtacccgcct tgtgaaagcc ctgccagcgc
    11101 gcgaccatcg gcatctttac ggacattaaa acgcttgcta atcagcggga taatatcgtt
    11161 catcagttcg cgatctgccg ctttagcatt cagcggataa aagactttac gtctttcctg
    11221 aggcacgaaa tcttcgggaa taatgccctt cgcatcggtt tcggtatccg ggatcaccac
    11281 cagcatcggt ttaattttcc cttcagccag caggttatcc atgatttgcg ggatacgccc
    11341 ctgatcgata gcggaacgtc cggtatcacc aaaaccgtga tagaaataga gcactggcaa
    11401 aggctcgccc atgccggtgt aacctggcgg ggtccagaca tacatctgac gttcagattg
    11461 caatgcgttg gagtggtaag ttatggcgat caaatcacca tgcgcaacag aacgcgtatc
    11521 cagataactg cctggcacca gaatcatact ggagttaacc tggcgctgag gcttagtcat
    11581 tgcggtgcct gtatcaatgc tgcgtacacc atcaacattg aaaaaatact cgtacaggtt
    11641 gcctttcagg ataggtgtgc gccacgacca gactcctgct tcgtctttgg tcatcgggtg
    11701 aatattgtcc ggaaccggaa cacccaccac tacagagaca ttttttgccc caggggcaaa
    11761 gtagcgaaag gtcacgctgt tatcggcatt gacctgagtc acatactgtt tcaccgggat
    11821 agtgggtgcc gggctggctg gcatatccgc tgctatggcc cactgagcag aaataccgct
    11881 ggcaatagcc agcgttaaag cagcaatttt tatattcatc gtaatattcc tttcattaag
    11941 ttccattacc accagatttc agcctgggca ccgacagcga actggtcgtc tttattgtct
    12001 tcgaaggtga agccatccag ttcgttttcc agggctttga tatacgtggc gtagaaacga
    12061 atttccggac gcgaggtcaa catactggta ttgactttaa aggtatggaa gagcgtggtt
    12121 ttataaccag actcattaaa tttattacta ttcgcatctt tgttttgctg ggtgaaatag
    12181 cccagttcaa cacctgtctg gttatattgg tcccaaatat aggccgggcg aacaaccgca
    12241 cgaatagatt cgaaatcaga gtgtgcgcct gtttcgtagc tataaatatc gttaccgaag
    12301 gagtaaacaa tcgcgttagc cacaataaaa tgatcgccga tataggcttc gccctgtgaa
    12361 gtcagacgaa ctgctgttcc gccggtgtga tcaccataat aacgaccatt aaaggtggta
    12421 aatggacttg cgccagcata acggccaaag ttactggcga tagagttatt cgcaaccagg
    12481 aaggagaatt cgttgaagcc acctttatca aatttctgcg ttaaagatgt gccaaacatc
    12541 caggtatctt tccagtcata atagccgtta ttatcctgat tatctttttc cgatgcgctt
    12601 tcgtttgccg tgacataacg accactcacc attaaggtcg ctttatccca taacgggata
    12661 tctttatagc gtaaatcaat agtattggta ttaatctgct gtttgttttg caggctgcga
    12721 tcgtaatcat cgacatcttc gcgaaccagc gcgatatcaa ttttacccgg accgactttc
    12781 cagttttcca gacctacacc cgctgcggca tcagtacgct gcgttttcca gtccagcatc
    12841 tggatttcaa ttttcggcgc accgtgttta cccacccaga aatcagcctc tggcgcaaag
    12901 ggcaggaaac ctttggtggt aacgtacata tcggagaact gcataaagtt ctcgccaccg
    12961 gcgttatcgc caaaccagcc ggtagagtac tgctgaccaa cgttaccatc catcatcaca
    13021 acggcatcaa cccgtttgcc gttttcgttg tagacacgtt gttttaactg caaatcaaac
    13081 cagccggagt attcgttacc aaagcggccc agagaaccaa tcgcccatga tttaggtgaa
    13141 ccatgagagg cggtccccca accagaacgg taatagccgt tgtaactaaa gccaatttca
    13201 tctttcacaa acttactgaa atctttcaga gtcatggaag aataaatcga gcctgtctca
    13261 ctggcatttt tctcttcatt tttgaccagt acagcatcag gttttgcggc actactggtc
    13321 gggaacggat tggcggcata cccttgatca ttcgtactta cgctacgatt caccgtcgct
    13381 ggcgtatatt ttttcttctc ttcatcttta tactttttga gttcgctttg cgtttctctt
    13441 aacgcttttt ctaataactc aaggcgatgt tccaccgtta atgattcagc caatgattgt
    13501 gcagaaaagg ctaacggtgc cattaataag atggcagagg taataagatt tcgtctaaac
    13561 ataatatccc tttatggtgc aaagaaagaa ttaacgtatc gcatccagac tgttctgaat
    13621 gcgacgataa ttaaggtgct ttattggtta tttttttaat gacagccccc gcgttttgat
    13681 cacttctgca taccatccga agcttttctt acgcgttctt gtgaggcttc cttcgccatt
    13741 atcatcacga tccacataaa taaagccgta gcgcttagac atttgtgaat gagatgcact
    13801 gactaaatca attggccccc aactggtgta ccccataata tccacaccat cggcaatcgc
    13861 ttcatttacc tgtaccaggt gatcgtttaa ataggcaatt cgataatcgt cctgtatcga
    13921 accatccgct tcaacgctgt cttttgcgcc taatccgttc tcgacaataa ataacggttt
    13981 ttgataacga tcccaaagcg tatttaacag aacccgtaat ccaaccgaat caatttgcca
    14041 cccccactct gaacttttca gatgcggatt ggggatcata ttcagtatgt tgccctgcgc
    14101 atttttatta atgctttcgt cgtgggaaac acaaccagtc atgtaataac taaaagagat
    14161 gaaatcgacg gtatgtttta aatcttctgc gtcactttca gtcatctcaa tggtgatatt
    14221 gtggtcgcgg aagaaacgct gcatatagcc gggatactgg ccacgcgcct gaacatcacc
    14281 aaagaacatc cagcgccggt tctcttccat ggcctgcaac atatcctgtg gctggcaggt
    14341 gagggggtaa accagcccac cgagaagcat attgccgatt ttcgcttcgg ggagcaggct
    14401 atgacaggct ttaactgccc gcgcactggc aaccagttga tggtggatag cctgataaac
    14461 ttccgcctcg ccactctctt ctgccagccc cacgcccgtg aatggctcgt gtaacgacat
    14521 gttgatttca ttaaacgtca gccataacgc cactttatgt tggtagcgag taaagaccgt
    14581 gcgggcgtaa tgctcgaagt gatcgatgac cgctcgatta gcccaaccgc cgtagttttt
    14641 caccagccca tatggcattt cgtaatggga taacgttacc agcggcttga tccccgcctg
    14701 cgccatttca tcaaacagcc gatcgtaaaa cgctaacccc gcttcattcg gttcgacttc
    14761 gtcgccctga gggaaaattc gcgcccaggc aatggaaata cgcagacagg tgaagcccat
    14821 ctcggcaaat aacgcgatat cttccgggta acggtgataa aaatcgatgg cgacatcttt
    14881 gatattctct ttccccagga tacgcggttc catttttccc attacgccat gaggctgtaa
    14941 atctgaggtc gagatccctt tgccatcttc ctgccaggca ccttccacct gattggcagc
    15001 tgttgcgccc ccccaaagaa atgtttctgg aaatgctttc ataattaact ccttttatcg
    15061 ttagcgaatg atggataaca gcggttcacc tgcgcttatc tgcgccgtgc cgtggggtaa
    15121 tacgtccgta aaatcatcgc tattactgat taataccggc gtcgtcagat caaatccggc
    15181 ctcgcgaata gcagggatat caaaagaaat cagccgatcg cctgtattga ccttgtcacc
    15241 cacgttgacg tgagcggaaa agaatttgcc gtccagtttt acggtgtcga taccgacatg
    15301 aatcaggagc tccacaccat catctgactc aatgccaatg gcgtgtaatg tggcgaacaa
    15361 cgaagcaatt cgacccgcaa ccggagaacg cacttcacca accgagggca gaatggcgat
    15421 acctttaccc aacaggccac tggcaaacgt ggtatcagcg acgtgaatga gcggcacaat
    15481 ctctcccgtc atcggtgaac agataccgcc ctgctcaggt ggtgtaataa cctctggtgt
    15541 tttctcttgc ggggcaccct gcgctggctg acgtttagcg gtgatgaaat gaagcatcac
    15601 cgtaccgaca aatgcgcaac cgatggcaat gacaccgcca ataacgctgg cccagacggt
    15661 gaaatcaatt cccgttgacg ggatggtttg catgaaggtg aaaatacttg gcaaaccaaa
    15721 ggagtacact ttcgtttgcg cgtagccaat gatggtggcc cccaaagccc cactgataca
    15781 ggcgataaca aaggggtact tacgcggcag attaacgcca tataccgctg gttcggtgat
    15841 accaaacagg cccgtcaacg ccgctgatcc cgccaccact tttttctgcg catcgcgttc
    15901 gcagaggaag acgccgagcg ccgccccgac ctgcgccata atggcgggca ttaacagcgg
    15961 gatcatggtg tcgtagccca gcacggtgaa gttattgata cacagcggca ccaggcccca
    16021 gtgcagtccg aacatgacga agatttgcca gaagccgccc attaccgcgc ctgcaaatgc
    16081 aggaaccgcc tgataaagcc agagataacc ggcggcaatc agttcgctta tccaggttga
    16141 tagcggcccc accagcagaa aggtgacggg tgtgataacc atcagacata gcaatggtgt
    16201 gaagaaattt ttgattgccg acggtaacca cgcattaagt cggcgttcca gaatgctgca
    16261 caaccaggca gaaaaaataa tgggaataac cgatgacgag taattcaaca atgtgaccgg
    16321 aatacccagg aaatccagcc ccagctcatc cgctttttgc ccgttctcga aagcagtcag
    16381 aattaatgga tgcactaacg ctccaccaat caccatggca gtaaatggat taccgccgaa
    16441 gcgtttcccc gcggtgtatc ccaggattat cgggaagaac caaaacaagg catcactggc
    16501 gctgaataaa attaaataag taccactttg ttcggtcgtc cactgaaaag tgagcgccag
    16561 agccagcata cctttcagga tcccggttgc cgccatcaaa ccgatcagag gcgtaaaaat
    16621 acctgaaata acataaacaa agcggtttag cagattacct ttctcatcat tttccggtgc
    16681 ctgttgcgct ttttcgccaa ggcctgccac gctgttgacc gccaggaaga catcggccac
    16741 atggttacct atgaccacct gaaactgacc accgctttcc accaccataa taataccggg
    16801 ggtctttttc agtacctcag cttgcgcttt gctttcatcc tttaacttaa aacgtaatcg
    16861 cgttgcgcaa tgcatcagac tcacaatgtt atctgcgccc ccgactcctg cgactatttt
    16921 tctggctaac tccgtcataa cttgccctct accgctttgc ggcaaaactc caaaaaaaaa
    16981 cctgaaaaaa acggcctgac gtgaatcaag caattttttt caggttttgc ccgcttagtg
    17041 cggtaacaat cctttactca gtaataatat ttcagtgttc tttgcgcacg cgctctatat
    17101 ttatggctaa aaacataatc tctgcgggtg aaattttacg ttgatactgc aaaccaataa
    17161 aaatggcgat ccgttccgca cattgccatg cttgcgggta attttgtttt actgcttgtt
    17221 gtaatgattc atcactatcg ttaattgaag catgttcaag aatacgccag gataaaaact
    17281 tcagatgtgt aaccagtcgc tgataactca agctttcttc ctggtaatta aggctgaact
    17341 gaaattttat taattgcagc atttcgcgca ttaactgcgt gacacctgca acatcctcca
    17401 tatttccgct catttgggca ctgaccagat gcatggcaat aaagcccact tcatctttcg
    17461 gtaactgcac gcccaaccgt ttatcaataa tggttaacgc ttcttcccct agctggaact
    17521 ctttcgggta aagccgctgg atatcccaca gcaacgggtt gggcagcaac acgttttgct
    17581 gaaagcgttt aatcgcaaac tggcaatggt cagttagcga gatataaata ctgtcctgta
    17641 attttcccaa gcgctcctgc gctaaagaga taatacgatc acaggttgcc atcacctcaa
    17701 gaggaatatg acttaagagt tcgcttaatc gcccgttcag ttcatgactg ctcaaggcat
    17761 actctttttc tattccactt gagttaattc tttcgccagc gcgtttttga aagccaattc
    17821 ctcgccccat gacgactttt tcccgctgtt gatcatcaat aaccaccaca acattattgt
    17881 tgagaatttt ggtgatttgc atgttcatag caaggacctt ttttataaac aaaaaaaccc
    17941 gacttcacca gtattctctg gttatgtcag gttttgcctg cgaatgcag
//
LOCUS       DAZKHF020000077        17899 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2201S31241
            SAMN43267800-rid25106413.denovo.077, whole genome shotgun sequence.
ACCESSION   DAZKHF020000077 DAZKHF020000000
VERSION     DAZKHF020000077.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267800
            Sequence Read Archive: SRR30307263
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 17899)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17899)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 17899)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350581.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350581.2
            Genome Coverage       :: 151x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:21:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,652
            CDSs (total)                      :: 5,557
            Genes (coding)                    :: 5,320
            CDSs (with protein)               :: 5,320
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 237
            CDSs (without protein)            :: 237
            Pseudo Genes (ambiguous residues) :: 0 of 237
            Pseudo Genes (frameshifted)       :: 102 of 237
            Pseudo Genes (incomplete)         :: 130 of 237
            Pseudo Genes (internal stop)      :: 52 of 237
            Pseudo Genes (multiple problems)  :: 41 of 237
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17899
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267800-rid25106413.denovo.077"
                     /serotype="O 26:H11"
                     /isolate="2201S31241"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-01"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            78..419
                     /gene="symE"
                     /locus_tag="ACHOSC_RS10760"
                     /old_locus_tag="pgaptmp_002152"
     CDS             78..419
                     /gene="symE"
                     /locus_tag="ACHOSC_RS10760"
                     /old_locus_tag="pgaptmp_002152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endoribonuclease SymE"
                     /protein_id="HGU4548088.1"
                     /translation="MTDTHSIAQPFEAEVSPANNRQLTVSYASRYPDYSRIPAITLKG
                     QWLEAAGFATGTAVDVKVMEGCIVLTAQPPTAEESELMQSLRQVCKLSARKQKQVQDF
                     IGVITGKQKVA"
     gene            complement(466..1629)
                     /locus_tag="ACHOSC_RS10765"
                     /old_locus_tag="pgaptmp_002153"
     CDS             complement(466..1629)
                     /locus_tag="ACHOSC_RS10765"
                     /old_locus_tag="pgaptmp_002153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1524 domain-containing protein"
                     /protein_id="HGU4548089.1"
                     /translation="MALGKESDKSLATAFQDLRELKVDVAYPFLLALYHDYKNDDLSH
                     EDFLSIIRLIESYVFRRAVCAIPTNSLNKTFATFYKVINKEKYLESIQVHFMNLPSYR
                     RFPNDDEFKRELKVRDLYNFRSRSYWLRRLENDKRRERVEEFTIEHIMPQNENLSAKW
                     REELGSDWQRVHKELLHTLGNLTLTRYNSRYSDRPFAEKRDIEDGFKHSPLYLNIGLG
                     QCEKWDEAAIRARADRLADLAVQVWQAPSLSEEVLAVYRGQPENKTSYSLSDYPFLAD
                     GSHSRVLFDHLRDEVMRLDAGITQEVLKLYIAFKAETNFVDVVPQKSRLRLSLNMQFH
                     ELVDPKGIAKDVTNVGRWGNGDVEIGFSDLAQLPYIMGLIRQAFEKQMESALV"
     gene            complement(1677..2559)
                     /locus_tag="ACHOSC_RS10770"
                     /old_locus_tag="pgaptmp_002154"
                     /pseudo
     CDS             complement(1677..2559)
                     /locus_tag="ACHOSC_RS10770"
                     /old_locus_tag="pgaptmp_002154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313331.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF262 domain-containing protein"
     gene            complement(2656..2820)
                     /gene="yjiS"
                     /locus_tag="ACHOSC_RS10775"
                     /old_locus_tag="pgaptmp_002155"
     CDS             complement(2656..2820)
                     /gene="yjiS"
                     /locus_tag="ACHOSC_RS10775"
                     /old_locus_tag="pgaptmp_002155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008786105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1127 domain-containing protein"
                     /protein_id="HGU4548090.1"
                     /translation="MEFHENKAKTPFIRLVQLWQAVRRWRRQMQTRRVLQQMSDERLR
                     DIGLRREDVE"
     gene            2997..4409
                     /gene="yjiR"
                     /locus_tag="ACHOSC_RS10780"
                     /old_locus_tag="pgaptmp_002156"
     CDS             2997..4409
                     /gene="yjiR"
                     /locus_tag="ACHOSC_RS10780"
                     /old_locus_tag="pgaptmp_002156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HGU4548091.1"
                     /translation="MTRYQHLATLLAERIEQGLYRHGEKLPSVRSLSREHGVSISTVQ
                     QAYQTLETMKLITPQPRSGYFVAQRKAQPPVPPMTRPVQRPVEITQWDQVLDMLVAHS
                     DSSIVPLSKSTPDVETPSLKPLWRELSRVVQHNLQTVLGYDLLAGQRVLREQIARLML
                     DSGSVVTADDIIITSGCHNSMSLALMAVCKPGDIVAVESPCYYGSMQMLRGMGVKVIE
                     IPTDPETGISVEALELALEQWPIKGIILVPNCNNPLGFIMPDARKRAVLSLAQRHDIV
                     IFEDDVYGELATEYPRPRTIHSWDIDGRVLLCSSFSKSIAPGLRVGWGAPGRYHDKLM
                     HMKYAISSFNVPSTQMAAATFVLEGHYHRHIRRMRQNYQRNLALYTCWIREYFPCEIC
                     ITRPKGGFLLWIELPEQVDMVCVARQLCRMNIQVAAGSIFSASGKYRNCLRINCALPL
                     SETYREALKQIGEAVYRAME"
     gene            complement(4651..5595)
                     /locus_tag="ACHOSC_RS10785"
                     /old_locus_tag="pgaptmp_002157"
     CDS             complement(4651..5595)
                     /locus_tag="ACHOSC_RS10785"
                     /old_locus_tag="pgaptmp_002157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001678496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rpn family recombination-promoting
                     nuclease/putative transposase"
                     /protein_id="HGU4548092.1"
                     /translation="MTNFTTSTPHDALFKSFLTHPDTARDFMEIYLPKDLRELCDLDS
                     LKLESASFVDEKLRALHSDILWSVNTREGDGYIYVVIEHQSREDIHMAFRLMRYSMAV
                     MQRHIEHDKRRALPLVIPMLFYHGSRSPYPWSLCWLDEFADPTTARKLYTAAFPLVDI
                     TVVPDDEIVQHRRVALLELIQKHIRQRDLMGLIDQLVVLLVTECANDSQITALLNYIL
                     LTGDEARFKKFISELTRRMPQHRERIMTIAERIYNDGWLLGMEKGKEEGEQRLLRLLL
                     QNGADPEWIQKITGLSTEQMQALEQPLPEIKRDPWIEY"
     gene            6062..7294
                     /gene="mdtM"
                     /locus_tag="ACHOSC_RS10790"
                     /old_locus_tag="pgaptmp_002158"
     CDS             6062..7294
                     /gene="mdtM"
                     /locus_tag="ACHOSC_RS10790"
                     /old_locus_tag="pgaptmp_002158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter MdtM"
                     /protein_id="HGU4548093.1"
                     /translation="MPRFFARHAATLFFPMALILYDFAAYLSTDLIQPGIINVVRDFN
                     ADVSLAPAAVSLYLAGGMALQWLLGPLSDRIGRRPVLITGALIFTLACAATMFTTSMT
                     QFLIARAIQGTSICFIATVGYVTVQEAFGQTKGIKLMAIITSIVLIAPIIGPLSGAAL
                     MHFVHWKVLFAIIAVMGFISFVGLLLAMPETVKRGAVPFSAKSVLRDFRNVFCNRLFL
                     FGAATISLSYIPMMSWVAVSPVILIDAGGLTTSQFAWTQVPVFGAVIVANAIVARFVK
                     DPTEPRFIWRAVPIQLVGLALLIIGNLLSPHVWLWSVLGTSLYAFGIGLIFPTLFRFT
                     LFSNNLPKGTVSASLNMVILMVMSVSVEIGRWLWFNGGRLPFHLLAVVAGVIVVFTLA
                     GLLNRVHQHQAAELAEER"
     gene            7335..8615
                     /gene="yjiN"
                     /locus_tag="ACHOSC_RS10795"
                     /old_locus_tag="pgaptmp_002159"
     CDS             7335..8615
                     /gene="yjiN"
                     /locus_tag="ACHOSC_RS10795"
                     /old_locus_tag="pgaptmp_002159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF445 domain-containing protein"
                     /protein_id="HGU4548094.1"
                     /translation="MNKLIELRRAKMLALSLLLIAAATFVVTLFLPPNFWVSGVKAIA
                     EAAMVGALADWFAVVALFRRVPIPIISRHTAIIPRNKDRIGENLGQFVQEKFLDTQSL
                     VALIRRHEPALLIGNWFSQPENARRVGQHLLQIMSGFLELTDDARIQRLLKRAVHRAI
                     DKVDLSGTSALMLESMTKNDRHQVLLDTLIAQLIALLQRDKSRKFIAQQIVRWLESEH
                     PLKAKILPTEWLGEHSAELVSDAVNSLLDDISRDRAHQIRHAFDRATFALIDKLKNDP
                     EMAARADAVKSYLKEDEAFNRYLSELWGDLREWLKADINSEDSRVKERIARAGQWFGE
                     TLIADDALRASLNGHLEQAAHRVAPEFSAFLTRHISDTVKSWDARDMSRQIELNIGKD
                     LQFIRVNGTLVGGCIGLILYLLSQLPALFPLGNF"
     gene            8731..9882
                     /gene="yjiM"
                     /locus_tag="ACHOSC_RS10800"
                     /old_locus_tag="pgaptmp_002160"
     CDS             8731..9882
                     /gene="yjiM"
                     /locus_tag="ACHOSC_RS10800"
                     /old_locus_tag="pgaptmp_002160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418755.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="double-cubane-cluster-containing anaerobic
                     reductase"
                     /protein_id="HGU4548095.1"
                     /translation="MSLVTDLPAIFDQFSEARQKGFLTVMDLKERGIPLVGTYCTFMP
                     QEIPMAAGAVVVSLCSTSDETIEEAEKDLPRNLCPLIKSSYGFGKTDKCPYFYFSDLV
                     VGETTCDGKKKMYEYMAEFKPVHVMQLPNSVKDDASRALWKAEMLRLQKAVEERFGHE
                     ISEDALRDAIALKNRERRALANFYHLGQLNPPALSGSDILKVVYGATFRFDKEALINE
                     LDAMTARVRQQWEEGQRLDPRPRILITGCPIGGAAEKVVRAIEENGGWVVGYENCTGA
                     KATEQCVAETGDVYDALADKYLAIGCSCVSPNDQRLQMLSQMVEEYQVDGVVDVILQA
                     CHTYAVESLAIKRHVRQQHNIPYIAIETDYSTSDVGQLSTRVAAFIEML"
     gene            9892..10659
                     /gene="yjiL"
                     /locus_tag="ACHOSC_RS10805"
                     /old_locus_tag="pgaptmp_002161"
     CDS             9892..10659
                     /gene="yjiL"
                     /locus_tag="ACHOSC_RS10805"
                     /old_locus_tag="pgaptmp_002161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418754.2"
                     /note="YjiL, as found in Escherichia coli, is a homolog of
                     the activator ATPases of enzymes such as lactoyl-CoA
                     dehydratase, (R)-phenyllactate dehydratase, and
                     2-hydroxyisocaproyl-CoA dehydratase. The typical substrate
                     of those enzymes is acyl-CoA with an OH at the
                     beta-position. YjiL is the putative activator for the
                     cognate protein YjiM, a putative 2-hydroxyacyl-CoA
                     dehydratase with unknown specificity, encoded by the
                     adjacent gene; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative 2-hydroxyacyl-CoA dehydratase activator
                     YjiL"
                     /protein_id="HGU4548096.1"
                     /translation="MTYSIGIDSGSTATKGILLADGVITRRFLVPTPFRPATAITEAW
                     ETLREGLETTPFLTLTGYGRQLVDFADKQVTEISCHGLGARFLAPATRAVIDIGGQDS
                     KVIQLDDDGNLCDFLMNDKCAAGTGRFLEVISRTLGTSVEQLDSITENVTPHAITSMC
                     TVFAESEAISLRSAGVAPEAILAGVINAMARRSANFIARLSCEAPILFTGGVSHCQTF
                     ARMLESHLGMPVNTHPDAQFAGAIGAAVIGQRVRTRR"
     gene            10656..10913
                     /gene="ytiA"
                     /locus_tag="ACHOSC_RS10810"
                     /old_locus_tag="pgaptmp_002162"
     CDS             10656..10913
                     /gene="ytiA"
                     /locus_tag="ACHOSC_RS10810"
                     /old_locus_tag="pgaptmp_002162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3343 domain-containing protein"
                     /protein_id="HGU4548097.1"
                     /translation="MKEFLFLFHSTVGVIQTRKALQAAGMTFRVSDIPRDLRGGCGLC
                     IWLTCPPGEEMQWVIPGLTESIYCQQDGVWRCIAHYRVSPR"
     gene            10978..11838
                     /gene="yjiK"
                     /locus_tag="ACHOSC_RS10815"
                     /old_locus_tag="pgaptmp_002163"
                     /pseudo
     CDS             10978..11838
                     /gene="yjiK"
                     /locus_tag="ACHOSC_RS10815"
                     /old_locus_tag="pgaptmp_002163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418753.3"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="SdiA-regulated domain-containing protein"
     gene            11906..13084
                     /gene="yjiJ"
                     /locus_tag="ACHOSC_RS10820"
                     /old_locus_tag="pgaptmp_002164"
     CDS             11906..13084
                     /gene="yjiJ"
                     /locus_tag="ACHOSC_RS10820"
                     /old_locus_tag="pgaptmp_002164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HGU4548098.1"
                     /translation="MPSSSHPVERFSFSTALFGMLVLTLGMGLGRFLYTPMLPVMMAE
                     GAFSFSQLSWIASGNYAGYLAGSLLFSFGAFHQPSRLRPFLLVSALASGLLILAMAWL
                     PPFILVLLIRFLAGVASAGMLIFGSTLIMQHTRHPFVLAALFSGVGIGIALGNEYVLA
                     GLHFALSSQTLWQGAGALSGMMLIALTLLMPSKKHAIAPMPLAKTEQQIMSWWLLAIL
                     YGLAGFGYIIVATYLPLMAKDAGSPLLTAHLWTLVGLSIVPGCFGWLWAAKRWGALPC
                     LTANLLVQAISVLLTLASDSPLLLIISSIGFGGTFMGTTSLVMTIARQLSVPGNLNLL
                     GFVTLIYGIGQILGPALTSMLGNGTSALAGATLCGAAALFIAALISTVQLFKLQVVTS
                     "
     gene            complement(13097..13651)
                     /gene="kptA"
                     /locus_tag="ACHOSC_RS10825"
                     /old_locus_tag="pgaptmp_002165"
     CDS             complement(13097..13651)
                     /gene="kptA"
                     /locus_tag="ACHOSC_RS10825"
                     /old_locus_tag="pgaptmp_002165"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001151849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA 2'-phosphotransferase"
                     /protein_id="HGU4548099.1"
                     /translation="MAKYNEKELADTSKFLSFVLRHKPEAIGIVLDREGWADIDKLIL
                     CAQKAGKRLTRTLLDTVVATSDKKRFSYSSDGRCIRAVQGHLTSQVAISFAEKTPPQF
                     LYHGTASRFLDEIKKQGLIAGERHYVHLSADEATARKVGARHGSPVILTVKAQEMAKR
                     GIPFWQTENGVWLTSTVAVEFLEW"
     gene            13901..14584
                     /gene="yjiH"
                     /locus_tag="ACHOSC_RS10830"
                     /old_locus_tag="pgaptmp_002166"
     CDS             13901..14584
                     /gene="yjiH"
                     /locus_tag="ACHOSC_RS10830"
                     /old_locus_tag="pgaptmp_002166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418750.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside recognition domain-containing
                     protein"
                     /protein_id="HGU4548100.1"
                     /translation="MTQQGDAVAGDLATEKVGIKGYLAFFLTIIFFSGVFSGTDSWWR
                     VFDFSVLNGSFGQLPGANGATTSFRGAGGAGAKDGFLFALELAPSVILSLGIISITDG
                     LGGLRAAQQLMTPVLKPLLGIPGICSLALIANLQNTDAAAGMTKELAQEGEITERDKV
                     IFAAYQTSGSAIITNYFSSGVAVFAFLGTSVIVPLAVILVFKFVGANILRVWLNFEER
                     RNPTQGAQA"
     gene            14581..15042
                     /gene="yjiG"
                     /locus_tag="ACHOSC_RS10835"
                     /old_locus_tag="pgaptmp_002167"
     CDS             14581..15042
                     /gene="yjiG"
                     /locus_tag="ACHOSC_RS10835"
                     /old_locus_tag="pgaptmp_002167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjiG family protein"
                     /protein_id="HGU4548101.1"
                     /translation="MTTQVRKNVMDMFIDGARRGFTIATTNLLPNVVMAFVIIQALKI
                     TGLLDWVGHICEPVMALWGLPGEAATVLLAALMSMGGAVGVAASLATAGALTGHDVTV
                     LLPAMYLMGNPVQNVGRCLGTAEVNAKYYPHIITVCVINALLSIWVMQLIV"
     gene            15055..16227
                     /gene="iadA"
                     /locus_tag="ACHOSC_RS10840"
                     /old_locus_tag="pgaptmp_002168"
     CDS             15055..16227
                     /gene="iadA"
                     /locus_tag="ACHOSC_RS10840"
                     /old_locus_tag="pgaptmp_002168"
                     /EC_number="3.4.19.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008798 - beta-aspartyl-peptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-aspartyl-peptidase"
                     /protein_id="HGU4548102.1"
                     /translation="MIDYTAAGFTLLQGAHLYAPEDRGICDVLVANGKIIAVASNIPS
                     DIVPDCTVVDLSGQILFPGFIDQHVHLIGGGGEAGPTTRTPEVALSRLTEAGVTSVVG
                     LLGTDSISRHPESLLAKTRALNEEGISAWMLTGAYHVPSRTITGSVEKDVAIIDRVIG
                     VKCAISDHRSAAPDVYHLANMAAESRVGGLLGGKPGVTVFHMGDSKKALQPVYDLLEN
                     CDVPISKLLPTHVNRNVPLFEQALEFARKGGTIDITSSIDEPVAPAEGIARAVQAGIP
                     LARVTLSSDGNGSQPFFDDEGNLTHIGVAGFETLLETVQVLVKDYDFSISDALRPLTS
                     SVAGFLNLTGKGEILPGNDADLLVMTPELRIEQVYARGKLMVKDGKACVKGTFETA"
     gene            16292..17204
                     /gene="hypT"
                     /locus_tag="ACHOSC_RS10845"
                     /old_locus_tag="pgaptmp_002169"
                     /gene_synonym="qseD"
                     /pseudo
     CDS             16292..17204
                     /gene="hypT"
                     /locus_tag="ACHOSC_RS10845"
                     /old_locus_tag="pgaptmp_002169"
                     /gene_synonym="qseD"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418747.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypochlorite stress DNA-binding transcriptional
                     regulator HypT"
     gene            complement(17197..17589)
                     /gene="iraD"
                     /locus_tag="ACHOSC_RS10850"
                     /old_locus_tag="pgaptmp_002170"
     CDS             complement(17197..17589)
                     /gene="iraD"
                     /locus_tag="ACHOSC_RS10850"
                     /old_locus_tag="pgaptmp_002170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313312.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anti-adapter protein IraD"
                     /protein_id="HGU4548103.1"
                     /translation="MMRQSRQAVLPEISGNKTSLLRKSVCSDLLTLFNSPHSSLPSLL
                     VSGMPEWQVHNQSDKHLQSWYCRQLRSALLFHEPRIAALQVNFKEAYCHTLAISLEIM
                     LYHDGEPLTFDLVWDNGGWCSAMLENVS"
     gene            complement(17586..17669)
                     /gene="idlP"
                     /locus_tag="ACHOSC_RS10855"
                     /old_locus_tag="pgaptmp_002171"
     CDS             complement(17586..17669)
                     /gene="idlP"
                     /locus_tag="ACHOSC_RS10855"
                     /old_locus_tag="pgaptmp_002171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iraD leader peptide IdlP"
                     /protein_id="HGU4548104.1"
                     /translation="MENEHQYSGARCSGQAAYVAKRQECAK"
     gene            complement(17785..>17899)
                     /gene="ytiD"
                     /locus_tag="ACHOSC_RS10860"
                     /old_locus_tag="pgaptmp_002172"
     CDS             complement(17785..>17899)
                     /gene="ytiD"
                     /locus_tag="ACHOSC_RS10860"
                     /old_locus_tag="pgaptmp_002172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="protein YtiD"
                     /protein_id="HGU4548105.1"
                     /translation="TPELSSGGDKYFHLRNYSEYSEYTSGFFLSLMIFIKS"
BASE COUNT         4182 a         4533 c         4486 g         4698 t
ORIGIN      
        1 gtatttagcg cggtgcggat gtgcgccaac acacccgcac cgctaatcac aatcactatt
       61 atcggagaat agcagttatg actgacacgc attctattgc acaaccgttc gaagcagaag
      121 tctccccggc aaataaccgt caattaaccg tgagttatgc gagtcgctac ccggattaca
      181 gccgtattcc cgccatcacc ctgaaaggtc agtggctgga agccgccggt tttgccaccg
      241 gcacggcggt agatgtcaaa gtaatggaag gctgtattgt cctcaccgcc caaccaccca
      301 ccgccgaaga gagcgaactg atgcagtcgc tgcgccaggt gtgcaagctg tcggcgcgta
      361 aacaaaagca ggtgcaggat tttattgggg taattaccgg taaacagaaa gtcgcctgat
      421 aaattgaccc ggtagcgcca gtgctaccgg gtattattcg ccgcgttata ccaacgcgct
      481 ctccatctgt ttttcaaatg cctgacgaat taatcccata atgtaaggaa gttgtgcgag
      541 gtcgctgaaa ccaatttcta catcgccatt accccagcgc ccaacatttg tcacatcttt
      601 ggcaatacct ttcggatcga ccagttcatg aaactgcata ttaagcgaca accgcagtcg
      661 gcttttttgc ggtacaacat caacaaaatt cgtttcagct ttaaacgcaa tatagagctt
      721 caatacttcc tgcgtgatcc ctgcgtccag gcgcataact tcatcgcgaa gatgatcgaa
      781 taacacccgg ctatgcgaac catcagcaag aaaaggataa tcactcaggc tgtaactggt
      841 tttgttttca ggctgccccc gataaacagc taaaacctct tcagaaagag aaggcgcttg
      901 ccagacctga accgcgagat cggccagacg atcggcacgg gcgcgaatgg cggcttcatc
      961 ccatttttcg cactgcccaa gaccgatatt caaatagagc gggctatgtt taaagccgtc
     1021 ttcaatatcg cgtttttccg cgaaaggtct gtcactgtag cgggagttat agcgcgttaa
     1081 agtgagattc cccaacgtat gtaacagttc tttatgcaca cgctgccagt cgcttcccag
     1141 ctcttcacgc catttagccg acagattttc gttctgcggc ataatgtgct caatcgtaaa
     1201 ctcttccacg cgctctctgc gtttatcgtt ttccagtctt cgtaaccagt agctgcggct
     1261 gcggaagtta tagagatcgc gaacttttaa ttcccgttta aattcatcat cgttggggaa
     1321 acgacgatat gaaggcaggt tcataaaatg tacctgaata ctttccagat atttttcttt
     1381 attaatgacc ttataaaaag tggcaaacgt cttgttcaaa gaattcgtcg gaattgcaca
     1441 tactgcacgg cggaaaacat aagattcaat taaacgaatt atgctcagga aatcttcgtg
     1501 agacaaatcg tcatttttat agtcatgata aagcgccagt aagaaaggat acgccacatc
     1561 aacctttaac tcgcgcaaat cctgaaaagc cgtagcaagc gatttgtcac tttcttttcc
     1621 caatgccatt gcacagtaat actccgcgta gatatgaata tcttcaacca gccgatctac
     1681 gcctttttca gcaacactct ggctgcgggc atggagttta aatgcctcat agacttcatc
     1741 tgtccgaggg atctcccccg tttttaccgt cagataatga cgcataaaac tgtcaaaata
     1801 ttcgctgtaa ccttgctgac caaaggcgac ttccattgga cgccagtgat cttcatacaa
     1861 ccgggtttga tgctctggtt cgaggcccat cagaataaaa ttgcgcacca gatcggcctg
     1921 acttaacgcc ttaccggtgg agttcatact ttcaaaaatc agttgcggat tatcctgacc
     1981 acgattaagc gccacatcga caattaataa ctttgctaac ccacgacata aggggatcaa
     2041 gtcatcaccg agtttggcaa tttgttcatc aaagaaagta aagttttcca ttattcgatg
     2101 ggaatagttt tctggcattg gtctttgttt tattaacgcc agtaaactgt ctttatcagt
     2161 ctcggtcagt agtaatttaa aacctttctc gccggactca taaggattga gcaaataata
     2221 attacgcaat ttcattgctg aaaagccatc aaaaacttcg tcttcgccaa gatggcgcga
     2281 taaagcctca atcagcaaca ttgcggtcgt cagccgttgt tgaccatcaa tgaccagtaa
     2341 cggagaaata ccagaaacct gatacaaccc ctgctcaata taaacaaccg aaccgataaa
     2401 atgcgctgat atatcgtcac gctttccggc acgaatgatg tcgtcccaaa gttgccgaca
     2461 ttgttgttct gtccatgaat aagtccgttg ataaatggga ataacaaact gctgcgaacg
     2521 ttttaaaaaa tcgagtaggc gggcttctgt agctttcatt ctgacgtctt tccgtgaagc
     2581 gatgatttcg caataataac cacttccttt tggaggttat agagaaatcc tgtttatccg
     2641 cgatgaattg ccccctcact ccacatcctc cctgcgtaac ccaatatctc tcaaccgctc
     2701 atcactcatc tgctgtaaca ctcgtcgggt ctgcatttgc cgccgccaac gcctcaccgc
     2761 ctgccagagt tgcacaagtc tgataaacgg cgttttggct ttgttttcgt gaaattccat
     2821 ctgctgcctc ctcatctttc agaggcttca tcatccacct tctggcagtt cacaatacag
     2881 atgcaaatat tagttttatt taacatacag acaggttaaa acagcatctg catccggttt
     2941 tttagcctca tctgtaccgg tttttctttc tgtatggtcc aaataaggaa tacagcatga
     3001 cgcgttatca acatctggcg actctacttg ccgagcggat tgagcaaggg ctgtatcgtc
     3061 atggggagaa attgccgtcg gtgcgtagct taagtcggga gcacggcgtc agcatcagca
     3121 ccgtgcagca ggcgtatcag acgctggaga cgatgaagct cataacccca cagccgcgtt
     3181 cgggttattt tgtcgcacaa cgtaaagccc agccgccagt gccgccgatg acgcgtccgg
     3241 tgcagcgccc ggtggaaatt acccagtggg atcaggtgct ggatatgctg gtggcgcata
     3301 gcgacagttc cattgttccg ttaagcaaaa gcacgccgga tgtcgaaacg cccagcctga
     3361 aaccgctctg gcgggagcta agccgggtgg tgcagcataa tctgcaaacc gtgctcggtt
     3421 atgacttgtt agccggtcag cgggtattga gagagcagat tgcccgcctg atgctcgaca
     3481 gcggctcggt ggtcaccgcc gatgacatca ttatcaccag cggctgccat aactcgatgt
     3541 cgctggcgtt aatggcggtg tgtaaaccgg gcgatattgt cgcggtcgaa tctccctgtt
     3601 attacggttc gatgcagatg ctgcgcggca tgggcgtgaa agtgattgaa atcccaaccg
     3661 atccagaaac aggcatcagc gttgaagcgc tggaactggc gctggaacag tggccgatta
     3721 aaggcatcat tctggtgcca aactgtaata atccgctggg atttattatg ccggacgcac
     3781 gcaaacgggc cgttctctct ctcgctcagc gtcatgatat tgtgattttt gaagatgatg
     3841 tctacggcga actggcaacg gagtatccgc gcccgcggac catccattca tgggatatcg
     3901 acgggcgagt gctgttgtgc agctcgttca gtaaaagtat tgctccaggc ctgcgcgtgg
     3961 gttggggcgc accggggcgt tatcacgata aactgatgca tatgaaatac gccatcagca
     4021 gctttaatgt gccgtccacg caaatggcgg cggcaacgtt tgtgctggaa ggtcactatc
     4081 atcgccatat ccggcggatg cggcagaact atcagcgcaa tttggcgctt tatacctgct
     4141 ggatacggga atattttccc tgcgaaatct gtattacgcg cccgaaaggc ggatttttac
     4201 tgtggataga attgcctgaa caggtcgata tggtctgcgt tgcgcggcag ctgtgccgca
     4261 tgaatatcca ggtggcagca ggctcgattt tctcggcttc cggcaaatac cgtaattgtc
     4321 tacgcatcaa ctgcgctttg ccgctcagcg aaacctatcg cgaagcacta aagcaaattg
     4381 gcgaggccgt gtatcgggca atggaataaa ttcgaattag tggcgaagca cctgatttcc
     4441 tctcacgaat cgcctgtaac tcgttgtatc tttgcagggt catcgccacg ttccagcatg
     4501 gcttaggcaa agtgtgcttc gttcataccg gatgcggcgt gaacgcctta tccggcctac
     4561 aaaccgcgca aattcaatag attgcagagg aaatgtaggt ctgataagcg tagcgcatca
     4621 ggcagctttg cgtttgtcat ccgtctctga ttagtattcg atccatggat cgcgcttgat
     4681 ttcaggcaag ggctgctcta atgcctgcat ttgctctgtc gaaagtccgg taatcttttg
     4741 tatccattca ggatctgccc cattctgcaa caacaatcta aggaggcgtt gttccccttc
     4801 ttctttcccc ttttccattc ccaacagcca tccatcatta taaattcgct ctgcaatcgt
     4861 cattattcgc tccctgtgtt gtggcattcg acgggtaagt tcgctgataa acttcttaaa
     4921 acgcgcttca tcgccagtca gtaaaatgta atttaacagc gcagttatct ggctgtcatt
     4981 agcacactct gtaaccagta atactaccag ttggtcgata agccccatca gatcgcgctg
     5041 gcgaatatgc ttttggatca actccaacag ggcgactctg cgatgctgca caatctcgtc
     5101 gtctggcacg acagtgatat ccaccagcgg gaacgcggcg gtataaagtt tccgtgcggt
     5161 agttgggtcg gcaaattcgt ccagccagca cagggaccag ggataaggac tacggctacc
     5221 gtgataaaat agcatcggta taaccagcgg tagcgcccga cgtttatcat gctcgatatg
     5281 gcgctgcatc accgccatgg aatagcgcat caggcgaaag gccatatgaa tatcctcacg
     5341 gctctgatgt tcaatcacca cataaatata gccatcgcct tcgcgggtat ttaccgacca
     5401 cagaatatcg gagtgtagcg cccgcaattt ttcatcgaca aagctggcgg attccagttt
     5461 taagctgtcg agatcgcaca gttcacgtaa atctttgggt aaatagatct ccataaaatc
     5521 ccgcgcggtg tcagggtggg tgagaaagga tttaaataat gcgtcatgcg gcgtgctggt
     5581 tgtgaagttt gtcatggcga tccgtcacct gttgataaga tggcccgact ttagcgacta
     5641 acaaaaccat tcacacctgg caatttcccg ttccccgagg cgctttccag gctatttttg
     5701 ttcctctgca agcgatacat ttttccggaa acaggctcgc gttttacatt tcctgtaccc
     5761 ggtagatatt cccctttcca ccgcgctctt ttgattaatc gcaaatttta attacactta
     5821 aggtgtatat tttctatgca aaccatcaat tcaagaggtg taatgtgctg atgactattt
     5881 gtaatcgtta tacattctga cccgaactca gaaggtattt ctctgtctgt ataagcacag
     5941 gcagtgtggt tgattacatg aaattcagta catttgtcct tctcgcctac ctttcgtcat
     6001 taccgacggt attgaatttc gttttccccg ttggggttct ccggacaagg agttgtttgt
     6061 tatgccacgt ttttttgccc gccatgccgc cacgctgttt ttcccgatgg cgttgatttt
     6121 gtatgatttt gccgcgtacc tgtcgacgga cctgattcag ccggggatca ttaatgtggt
     6181 ccgtgatttt aatgccgatg tcagtctggc cccggcagcc gtcagtctct atctcgcggg
     6241 cggtatggcg ttacagtggc tgctggggcc gctttccgac aggattggcc gcaggccggt
     6301 gctgattacc ggggcgctaa tatttaccct tgcctgcgct gcgacaatgt tcacaacgtc
     6361 tatgacacag tttcttatcg cgcgtgcaat tcagggcacc agtatctgtt ttattgctac
     6421 cgttggttat gtcacggtgc aggaggcgtt cggacagaca aaagggatca agttgatggc
     6481 gattatcacc tccatcgtac tgattgcgcc gattattggc ccgctttccg gcgcagctct
     6541 gatgcacttt gtgcactgga aagtcctttt tgccatcatt gcggttatgg gttttatctc
     6601 atttgttggc ttactgctgg cgatgccaga gacggtgaag cgcggcgcgg ttccatttag
     6661 cgccaaaagc gtcttgcgcg attttcgtaa tgtcttttgc aatcggctgt tcctctttgg
     6721 cgcagcaacc atctctttaa gctatatccc aatgatgagt tgggtggctg tctcgccggt
     6781 gatcctgatt gacgcaggcg gcttaacaac ttcgcagttc gcctggacac aggttccggt
     6841 gttcggcgcg gtgattgtcg cgaatgccat cgtggcgcgt ttcgttaaag atccgaccga
     6901 accgcgcttt atctggcgtg ccgtacccat tcaactggtc ggcctcgcac tgttgattat
     6961 cggcaatctg ctgtcaccgc acgtctggct gtggtcggtg ctgggcacca gtctgtatgc
     7021 tttcgggatt ggtttaatct tcccgacctt attccgcttt acactttttt ccaataactt
     7081 accgaaaggg accgtctccg catcgctgaa tatggtgatc ctgatggtga tgtctgtctc
     7141 ggtcgaaatc ggccgctggc tgtggtttaa cggcggtcgc ttgccgtttc atctgttagc
     7201 cgtcgtagcg ggagttatcg tcgttttcac cctggcgggg ttgctcaatc gcgtgcacca
     7261 acatcaggct gccgagctgg cggaggagcg gtgatttttg cgcgatcctg ccgtcaggct
     7321 ctattcttaa cgttatgaat aaactcattg aactcagacg cgccaaaatg ttggcgctct
     7381 ctttactgct tatcgccgct gctacctttg tcgttacgct gtttttgcca cccaattttt
     7441 gggtgagcgg cgtgaaggca attgctgaag cggcgatggt cggcgcgctg gcggactggt
     7501 ttgcggtggt ggcgctgttt cgccgcgtgc caattccgat catttctcgt catacggcga
     7561 ttatcccgcg taataaagac cggattggcg aaaatctcgg tcagttcgtg caggaaaaat
     7621 ttctcgatac ccagtcgctg gtggcattga ttcgacgtca cgaaccggcg ttgctaattg
     7681 gtaactggtt tagccagcca gaaaacgccc gccgcgttgg tcagcatctg ttgcagatca
     7741 tgagtggttt tcttgaactg accgatgatg cacgtattca gcgcctgctt aagcgcgcgg
     7801 tccatcgggc gattgataag gtcgatcttt ccggaaccag tgcgttgatg ctggagagta
     7861 tgaccaaaaa cgatcgtcat caggtgctgc tcgatacgct gatcgcacag ttgattgctc
     7921 ttctccagcg cgataaatcg cgcaagttta tcgcccaaca aattgttcgc tggctggaga
     7981 gtgagcatcc actgaaagcc aaaattctcc ccaccgaatg gctgggcgaa catagcgcgg
     8041 agttggtttc tgacgcggtg aattctttgc ttgatgatat cagccgcgat cgtgcgcatc
     8101 agatccgcca tgcatttgat cgcgccactt ttgccctgat cgacaagttg aaaaacgatc
     8161 cggaaatggc agcgcgagct gatgccgtaa aaagctatct gaaagaggat gaagccttta
     8221 accgctatct cagtgaattg tggggggatt tacgggaatg gctgaaagcg gatatcaaca
     8281 gtgaagattc tcgtgtgaaa gaacgtatcg cgcgggcggg tcaatggttt ggcgaaacgt
     8341 taattgccga tgatgccttg cgggcgtcgt taaatggtca tctggaacaa gccgcgcacc
     8401 gcgtcgcacc tgagttttcc gcattcctga cgcgccacat cagcgatacg gtaaaaagct
     8461 gggatgcccg ggatatgtcg cggcaaatag agttaaatat cggcaaagat ctgcagttta
     8521 tccgtgtcaa cggtacgctg gttggcggtt gtattgggct aattttatat ttgctgtcac
     8581 agctcccggc cttgttcccc ctcggcaatt tttagaaatc catgaaaaac taagtggcta
     8641 accgcgagag agatcaaata aaccacacta aaagaatggt aataatgtgg cctcttttca
     8701 ttatctcccg tggtacgggg aaggaaaatc atgtcacttg tcaccgatct acccgccatt
     8761 ttcgatcagt tctctgaagc tcgccagaaa ggctttctca ccgtcatgga tctcaaggag
     8821 cgcggcattc cgctggttgg cacttactgc acttttatgc cgcaagagat cccgatggca
     8881 gccggtgcgg ttgtggtttc gctctgttcc acctctgatg aaaccattga agaagcggag
     8941 aaagatctgc cgcgcaacct ctgcccgctg attaaaagca gctacggctt cggtaaaacc
     9001 gataaatgcc cctacttcta cttttctgat ctggtggtcg gtgaaaccac ctgcgacggc
     9061 aaaaagaaaa tgtatgaata catggcggag tttaagcctg ttcatgtgat gcagctgcca
     9121 aatagcgtta aagacgatgc ctcgcgtgcg ttatggaaag ccgagatgct gcgcttacaa
     9181 aaagcggtgg aagaacgttt tgggcacgaa attagcgaag atgctctgcg cgatgccatt
     9241 gctctgaaaa accgcgaacg tcgcgcactg gctaattttt atcatcttgg gcagttaaat
     9301 cctccggcgc ttagcggcag cgacattctg aaagtggttt acggcgcaac cttccggttc
     9361 gataaagagg cgttgatcaa tgaactggac gcgatgaccg cccgcgttcg tcagcagtgg
     9421 gaagaaggcc agcgactgga cccgcgtcca cgcattttaa tcaccggctg cccgattggc
     9481 ggcgcagcag aaaaagtggt gcgcgcgatt gaagagaatg gcggctgggt tgtcggttat
     9541 gaaaactgca ccggggcgaa agcgaccgag caatgcgtgg cagaaacggg cgatgtctac
     9601 gacgcgctgg cggataaata tctggcgatt ggctgctcct gtgtttcgcc gaacgatcag
     9661 cgcctgcaaa tgctcagcca gatggtggaa gaatatcagg tcgatggcgt agttgatgtg
     9721 attttgcagg cgtgccatac ctacgcggtg gaatcgctgg cgattaaacg tcatgtgcgt
     9781 cagcagcaca acattcctta tatcgctatt gaaacagact actccacctc agatgtcggg
     9841 cagctcagta cccgtgtcgc ggcctttatt gagatgctgt aaggagtggc agtgacatat
     9901 tcgattggca ttgattctgg ttctaccgcc accaaaggga tcttactggc agacggcgtg
     9961 attacgcgcc gtttcctcgt tccaacaccc tttcgtccgg caacagcaat tactgaagcc
    10021 tgggaaactc tgcgcgaagg gttagagaca acgccgtttc tgacgctcac cggctatgga
    10081 cggcaactgg tggattttgc cgataaacaa gtgacggaaa tctcctgtca tgggctgggc
    10141 gcacggtttc ttgcgccagc aacgcgcgcg gtaatcgaca tcggtggtca ggacagcaaa
    10201 gtgattcagc ttgatgatga cggtaacctg tgcgatttcc tgatgaatga caaatgcgcg
    10261 gcgggcaccg ggcgtttcct ggaggtgatc tcgcgcacgc ttggcaccag cgtcgagcaa
    10321 ctcgacagca ttaccgaaaa tgtcacgccg cacgccatca cgagtatgtg cacagtgttt
    10381 gctgaatcag aagcgatcag cctgcgctca gcgggcgtcg cgccagaagc gattctcgcc
    10441 ggagtgatta acgcgatggc gcggaggagt gccaatttca ttgctcgtct ctcctgtgaa
    10501 gcgccgattc tgtttactgg tggcgttagt cattgccaga cgtttgcccg gatgctggaa
    10561 tctcatctgg gaatgccggt aaatacccat cctgatgcgc aatttgctgg cgcaattggc
    10621 gcggcggtaa ttggtcaacg agtgaggaca cgccgatgaa agagttttta tttttatttc
    10681 actccacagt cggcgtcata caaacccgca aagcgttgca ggcagcgggc atgacttttc
    10741 gcgtcagcga tattccccgt gatttacgcg gcggctgcgg gttatgcatt tggctgacct
    10801 gtccccccgg cgaggaaatg caatgggtga tccctgggct gactgagtca atttattgtc
    10861 agcaggatgg tgtctggcgc tgcattgcac attaccgggt ttctccgcgt taaggattca
    10921 ttaaggtcgc tacgtatact tctcgatatc ttattgattt gccttaaagg gattaccgtg
    10981 acaaaaagta tttcgttaag taaacgcatc tccgttattg tgattctttt tgccattgtg
    11041 gcagtgtgta ctttcttcgt gcaatcttgc gcccgtaaaa gcaatcatgc agcataattt
    11101 cagaattacc acgccaccat tgacggcaaa gaaattgcag gcataacgaa taatatctct
    11161 tcgctgacct ggtcggcaca aagcaatact ctttttagca ctatcaacaa acctgccgcc
    11221 attgttgaaa tgaccaccaa tggtgatttt atccgcacaa ttcctctgga ttttgttaag
    11281 gatctcgaga cgatcgaata cattggcgat aatcagtttg tcattagcga tgagcgcgat
    11341 tacgccattt atgtgatttc actgacccca aactcggaag taaaaatcct caaaaaaata
    11401 aaaatcccgc tgcaagactc cccaaccaat tgcggttttg aagggttggc ttattctcgc
    11461 caggatcaca ctttctggtt ttttaaagag aaaaatccga ttgaggtcta caaagttaac
    11521 gggctgttaa gtagcaatga attacacatc agcaaagacg aggcactaca acggcaattt
    11581 actttagatg atgtgtccgg cgcagaattt aaccagcaaa aaaatacgct tctggtgctg
    11641 tctcatgaat ctcgcgcact ccaggaagta accctggttg gcgaggttat cggtgaaata
    11701 tcactaacca aaggtagtcg aggtttgtcg cataacatca aacaggccga agggattgcg
    11761 atggatgcct ctggtaatat ttatatcgtt agcgaaccaa atcgttttta tcgctttacc
    11821 cctaaatcat cgcattaaca ggctggcata tttcaccttt ttgttattaa cttgttaaca
    11881 acaaaaatga catacaaggt taaacatgcc ttcgtcctcg catcccgtag aacgcttttc
    11941 tttcagcacc gcgcttttcg ggatgctggt tctgacctta ggtatgggat taggtcgctt
    12001 cctttatacg cctatgttgc ccgtcatgat ggcggaaggg gcgttttcat ttagccagct
    12061 ctcgtggatt gccagcggca actatgcggg gtatctggct ggcagtctgc tattttcgtt
    12121 tggcgcattt caccagccgt cgcgcctgcg cccatttctg ttggtttctg ccttggcgag
    12181 cgggttgttg atcctcgcaa tggcatggtt gccgccattt attctggtgt tattgattcg
    12241 cttcctggcg ggtgtcgcca gcgccggtat gctgattttt ggttcgacgc tgattatgca
    12301 gcacacgcgc catccttttg tgctggcagc tttgttttct ggcgttggca ttggcattgc
    12361 actgggcaac gaatatgttc tggcaggcct gcattttgcc ttatcctcgc aaacgttatg
    12421 gcaaggcgcc ggggcgcttt ctggcatgat gctgattgca cttacgcttt taatgccctc
    12481 gaaaaaacac gccatcgcac caatgccatt ggcaaaaacg gagcaacaga taatgagctg
    12541 gtggttactg gctattctgt acggcctggc gggatttggt tatatcatcg tcgcgactta
    12601 cctgccgctc atggcgaaag atgcaggctc accattgtta accgcccatc tctggacgtt
    12661 agtcggctta tcaattgtgc caggctgctt tggctggcta tgggcagcaa aacgttgggg
    12721 ggctttgcct tgcctgacgg cgaatttgct ggtgcaggct atcagtgtgc tgcttactct
    12781 cgccagcgac tctcctctct tgcttatcat tagtagtatc ggttttggcg gtacctttat
    12841 gggcacgaca tctctggtga tgactatcgc ccgccaactg agcgttccgg gaaatctcaa
    12901 tcttttaggc tttgtgacgc tcatttacgg cattggacaa atcctcggcc cggcgctgac
    12961 cagtatgctc ggcaatggaa catcggcgct tgccggcgcc actctctgcg gcgcggcggc
    13021 gttgtttatt gctgcgctta tcagcacagt acaattattt aagctgcaag tggtcacttc
    13081 atgaggagtg tttcccttac cactcaagaa attcgaccgc aaccgttgat gtcagccaga
    13141 ccccgttttc cgtttgccag aaagggatgc ctcgtttcgc catttcctgt gctttgacgg
    13201 ttaagataac cggcgatccg tgtcttgctc ccactttgcg tgccgttgct tcatcagcag
    13261 agagatgtac gtagtgacgc tcacctgcaa tcagcccctg tttttttatc tcgtcaagga
    13321 aacggcttgc cgtaccgtgg taaagaaact gtggcggtgt tttttcggca aaggaaatag
    13381 cgacctgcga agtcaaatgc ccctgaaccg cgcggatgca cctaccatca ctggaataac
    13441 taaaacgctt tttatcactg gtggcgacca cggtatccag caatgtacgg gttaatcgct
    13501 tgcccgcttt ttgggcacaa agtatgagct tatcgatatc cgcccatcct tcacggtcaa
    13561 gtacgatacc aatcgcctcc ggtttgtgtc ggaggacaaa acttaaaaac ttgctcgtat
    13621 ccgccagctc tttttcgttg tactttgcca tctcgttttc gtccctggat tttctgcttt
    13681 tatttactga tgtcattgtc actgggttta tgaattttct gcaactcaat cctgcgcaga
    13741 gccttttcca cacagcgtaa tttttccgct atgcaaaaaa cgcatagcca gaaacatact
    13801 tgcattttcc ccatacccac atggccgata aggtttttca gctccaggaa ttttcctggc
    13861 tcaagcaaca cacaaacaca aaaaaacaat ggggattgtt atgacacaac aaggggatgc
    13921 tgtagccggg gatctggcga cagaaaaagt gggcattaaa gggtatctgg ctttttttct
    13981 caccataatc tttttttccg gtgttttttc cgggacagac agctggtggc gcgtttttga
    14041 tttttccgtt ctgaacggat cgtttgggca actgcctggt gcgaacggag ccaccacctc
    14101 atttcgcggg gcgggtggcg cgggggctaa agatggcttt ctctttgcgc tggagctggc
    14161 accgtcagtc attctctctc tggggattat ttcaattacc gatggtcttg gtggattacg
    14221 cgccgcgcag cagttaatga cacctgtgct aaaaccgttg ttgggcattc cagggatctg
    14281 ctcgctggcg ctgatcgcta acctgcaaaa cactgatgcc gccgccggta tgaccaaaga
    14341 gctggcgcag gaaggggaaa ttaccgaacg cgataaagtg attttcgccg catatcaaac
    14401 cagcggtagc gccatcatca ccaactactt ctcttccggc gtcgcggtgt tcgcctttct
    14461 gggcacgtcc gtaatcgtac ctttagccgt catcctggtg tttaaatttg tcggagccaa
    14521 cattttgcgc gtctggctta acttcgaaga acgtcgcaac ccaacgcaag gagcgcaagc
    14581 atgacaactc aggtccgcaa aaacgtcatg gatatgttta tcgatggcgc gcgccgtggc
    14641 tttactatcg ccacgaccaa cctgctgcca aacgtggtga tggcgtttgt catcattcag
    14701 gcgctgaaaa ttaccggcct gctcgactgg gtggggcata tctgtgaacc cgtaatggcg
    14761 ctgtggggat tacccggtga agcggcaacc gtccttttag ccgcattaat gagtatgggt
    14821 ggcgcagtcg gtgttgccgc cagtctggca accgctggcg cattaaccgg tcacgatgtc
    14881 accgttttac tgcccgcgat gtatttaatg ggcaacccgg tgcaaaatgt cggtcgctgc
    14941 ctggggacag ccgaagtgaa tgccaaatat tacccgcata tcattacggt ctgcgtgatt
    15001 aacgcattgc tgtcgatctg ggttatgcag cttattgttt aacaaggagt taccatgatt
    15061 gattataccg cagccggttt taccctgctg cagggagcgc atttgtatgc gccggaagat
    15121 cggggaattt gcgatgtcct cgtcgctaac ggcaaaatta tcgccgttgc cagcaatatc
    15181 ccttctgaca ttgtaccgga ctgcacggtt gtcgatctca gcgggcagat cctctttcct
    15241 ggttttattg atcaacacgt ccatttgatt ggtggtggcg gtgaagcagg tcccacgacg
    15301 cgcacgccag aagtggcgct aagtcgcctg acggaagcgg gcgtcacgtc agtggttggt
    15361 ctgctgggca ccgactctat ctctcgccac ccggaatccc tgctcgccaa gacccgtgcg
    15421 ctcaatgaag aaggcatcag cgcctggatg ctgaccggcg cttatcatgt cccttcccgc
    15481 accattacag gttccgtgga aaaagacgtg gcgattatcg atcgtgtgat tggcgtgaaa
    15541 tgcgccatct ctgatcaccg ttctgccgca ccggacgttt atcacctggc taatatggcg
    15601 gcggaatccc gcgttggcgg tttgctcggc ggtaaacctg gcgtcaccgt gttccacatg
    15661 ggcgacagca aaaaggcgtt acagcctgtc tatgacctgc tggaaaactg cgatgtgccg
    15721 atcagcaagt tgctgccgac ccacgttaac cgcaacgttc cgttgtttga gcaggcgctg
    15781 gagttcgcgc gcaaaggcgg cactatcgat atcaccagca gcattgacga accggtcgcc
    15841 cctgccgaag gtattgcccg cgccgttcag gcgggtattc cgctggcacg cgtcaccctc
    15901 agctccgacg gcaacggtag ccagccgttc ttcgatgacg aagggaattt aacccatatc
    15961 ggtgttgccg gttttgaaac gttgctggaa accgtgcagg tgctggtcaa agactatgat
    16021 ttcagtatca gcgatgccct gcgcccgctc accagtagcg tagccggttt ccttaacctg
    16081 accgggaaag gcgaaattct gccaggcaat gatgctgact tgctggtcat gacgccagaa
    16141 ctgcgcattg agcaggtata cgctcgcggc aaactgatgg tcaaagacgg caaagcctgc
    16201 gtgaaaggaa cgtttgaaac ggcttaaacg gttgtcagcc gcccgaatgg acgtagaatg
    16261 cccgcaaggg cggctgacag agtaaaacgt aatggatgac tgtggtgcga ttttgcataa
    16321 tattgaaacc aaatggcttt atgattttct gaccctggaa aaatgccgca atttttccca
    16381 ggcggcagtc agtcgcaacg tctcgcaacc ggcattcagc cgccgcatcc gtgcgctgga
    16441 acaggcgatt ggtgttgaat tgtttaaccg ccaggtaacg ccgctgcaac tctcagaaca
    16501 agggaaaatc ttccattcgc agatccgcca tctgttgcaa cagttagaga gcaatctggc
    16561 agagctgcgt ggcggcagcg attacgcgca acgtaaaatc aagatagccg ctgcacactc
    16621 tctttccctc gggctgttac cgtccattat cagccagatg ccgccgctct ttacctgggc
    16681 aattgaagct attgatgtcg atgaagcggt cgataaactg cgtgaagggc aaagtgactg
    16741 tattttttcc tttcacgacg aagatttgct ggaagcgccg tttgaccaca ttcgcttatt
    16801 tgaatctcaa ttgttccctg tctgcgccag tgacgaacac ggagaagcac tttttaacct
    16861 cgcgcagcca cactttccgt tactgaatta cagccgcaac tcctacatgg ggcgattgat
    16921 taatcgcacc ctgacgcgcc acagtgagtt aagtttcagc accttttttg tctcttcgat
    16981 gagcgagctt ttaaagcagg ttgccctcga cggctgtggg attgcctggc tgccggaata
    17041 cgctatccaa caagaaattc gcagcgggca gctcgttgtg ctgaatcggg acgaactggt
    17101 gatcccgatc caggatctac gcataccgga tgaatactcg catgaatccc gttgccgaac
    17161 gcttctggcg tgaactgcgc gagctggaga ttgtgcttag ctgacattct ccagcatcgc
    17221 actacaccaa ccgccattat cccagaccag atcaaacgtt agcggttcac cgtcgtggta
    17281 cagcattatc tcaaggctga tcgccagagt gtggcagtac gcttctttaa aattaacctg
    17341 caacgccgca atccgtggct catgaaataa cagtgcagaa cgcagctgac ggcaatacca
    17401 gctctggaga tgtttgtcgc tctggttatg cacttgccac tcgggcatgc ctgaaacaag
    17461 taatgacggt aatgacgaat gaggagaatt aaaaagagtg aggagatcgc tgcataccga
    17521 tttacgcagc aatgacgttt tattgcctga aatttcaggt aaaacagcct gacgtgattg
    17581 gcgcatcatt ttgcgcactc ctgacgttta gcaacatatg cggcttgccc tgaacaccgg
    17641 gcaccactgt attgatgctc attttccata gccattccct tttgttcatt acatccatgt
    17701 cagtctgaga caggcctgaa tcctgtcagc aaagtataaa accttacttg cctatgaata
    17761 tctactgact tttcaggaaa aaagtcatga tttgataaaa atcatcaaac tcaaaaaaaa
    17821 accgctagta tattctgaat attccgaata gttacgtaga tgaaaatact tgtcaccacc
    17881 gctacttaat tccggtgtg
//
LOCUS       DAZKHF020000078        17839 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2201S31241
            SAMN43267800-rid25106413.denovo.078, whole genome shotgun sequence.
ACCESSION   DAZKHF020000078 DAZKHF020000000
VERSION     DAZKHF020000078.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267800
            Sequence Read Archive: SRR30307263
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 17839)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17839)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 17839)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350581.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350581.2
            Genome Coverage       :: 151x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:21:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,652
            CDSs (total)                      :: 5,557
            Genes (coding)                    :: 5,320
            CDSs (with protein)               :: 5,320
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 237
            CDSs (without protein)            :: 237
            Pseudo Genes (ambiguous residues) :: 0 of 237
            Pseudo Genes (frameshifted)       :: 102 of 237
            Pseudo Genes (incomplete)         :: 130 of 237
            Pseudo Genes (internal stop)      :: 52 of 237
            Pseudo Genes (multiple problems)  :: 41 of 237
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17839
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267800-rid25106413.denovo.078"
                     /serotype="O 26:H11"
                     /isolate="2201S31241"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-01"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            complement(<1..613)
                     /locus_tag="ACHOSC_RS10865"
                     /old_locus_tag="pgaptmp_002173"
     CDS             complement(<1..613)
                     /locus_tag="ACHOSC_RS10865"
                     /old_locus_tag="pgaptmp_002173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4548106.1"
                     /translation="MAVNSNGSNNRVAGRDFHEKNIQIERYDGSHTVNIAIPSNNDDD
                     DRPLLKAQRKELNSLVAAIAEASNTEAFIIWQKVHAEIGVAGIDDMTVNQYKTAESFL
                     HAMLERCKDHDACKTLVSLLLRNSEDCGLRQKLLRYCHINFGTGRLNDLTRSQLQSAL
                     SWLEQQSASSHTESSALPEVRLRASELIRLYPKEIIFFICVGVL"
     gene            complement(629..1033)
                     /locus_tag="ACHOSC_RS10870"
                     /old_locus_tag="pgaptmp_002174"
     CDS             complement(629..1033)
                     /locus_tag="ACHOSC_RS10870"
                     /old_locus_tag="pgaptmp_002174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HGU4548107.1"
                     /translation="MKNDIGQRLREERERLGLSQVAMSDIGGVKKLTQLRYEKGDSFP
                     DAAYLAALSRFGLDVQYVVLGIHSPETYNDDEQELITRFRAASLDVKNAVIGALKGAI
                     SEKETQPSGRELNISGGNNRIAGRDYNETKGR"
     gene            complement(1123..1260)
                     /locus_tag="ACHOSC_RS10875"
                     /old_locus_tag="pgaptmp_002175"
     CDS             complement(1123..1260)
                     /locus_tag="ACHOSC_RS10875"
                     /old_locus_tag="pgaptmp_002175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_305354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4548108.1"
                     /translation="MRAMLEDERCSLAAISSMTARSSRDSRSASLGPVAVLRGMRDLV
                     S"
     gene            1332..1535
                     /locus_tag="ACHOSC_RS10880"
                     /old_locus_tag="pgaptmp_002176"
     CDS             1332..1535
                     /locus_tag="ACHOSC_RS10880"
                     /old_locus_tag="pgaptmp_002176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HGU4548109.1"
                     /translation="MSGITININVNAPYVSLQKYAEITGIPLNTCKKMLADGRIIIRP
                     KRAKMEKPEVNLVAMLKDALANS"
     gene            1557..1907
                     /locus_tag="ACHOSC_RS10885"
                     /old_locus_tag="pgaptmp_002177"
     CDS             1557..1907
                     /locus_tag="ACHOSC_RS10885"
                     /old_locus_tag="pgaptmp_002177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4761 family protein"
                     /protein_id="HGU4548110.1"
                     /translation="MKKNTNNPYSKFRNGVERHVHHVATSASRSNSRYNLNETHATPD
                     GHAVKQIGEHTWLIEKAGIVVHRCPHNPFTGNRIFALSSGDNQFGQDFTLYEALRTVD
                     RLLRGQSFIKQAAL"
     gene            1918..2196
                     /locus_tag="ACHOSC_RS10890"
                     /old_locus_tag="pgaptmp_002178"
     CDS             1918..2196
                     /locus_tag="ACHOSC_RS10890"
                     /old_locus_tag="pgaptmp_002178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4548111.1"
                     /translation="MTKDHAQGVFIRFIDFRGELLLRASAIDGVVPSEKNAATYVYLN
                     GTRLTVELPYQIIREIISEAEKSRQVNGDEPYIEIICMDSEAEIQKAD"
     gene            2208..2450
                     /locus_tag="ACHOSC_RS10895"
                     /old_locus_tag="pgaptmp_002179"
     CDS             2208..2450
                     /locus_tag="ACHOSC_RS10895"
                     /old_locus_tag="pgaptmp_002179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4754 family protein"
                     /protein_id="HGU4548112.1"
                     /translation="MDKEYKTLINKALERFYFRLSASGAHSERAARDSLTRAIRSLYD
                     VAFYADDLDALNELSELICAAECGEHIEPYKLGNIA"
     gene            2447..2560
                     /locus_tag="ACHOSC_RS10900"
                     /old_locus_tag="pgaptmp_002180"
     CDS             2447..2560
                     /locus_tag="ACHOSC_RS10900"
                     /old_locus_tag="pgaptmp_002180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4548113.1"
                     /translation="MSIFISWIVLIISVVCAIGIMRIINSVKKIERFFTGE"
     gene            2647..2850
                     /locus_tag="ACHOSC_RS10905"
                     /old_locus_tag="pgaptmp_002181"
     CDS             2647..2850
                     /locus_tag="ACHOSC_RS10905"
                     /old_locus_tag="pgaptmp_002181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4548114.1"
                     /translation="MAIKHFPVVRFTSRGREYEVDERLITTIDKHRSEKDAHHIYLTD
                     GTYFCATNVARVNLIRQVQDPRK"
     gene            3174..3563
                     /locus_tag="ACHOSC_RS10910"
                     /old_locus_tag="pgaptmp_002182"
     CDS             3174..3563
                     /locus_tag="ACHOSC_RS10910"
                     /old_locus_tag="pgaptmp_002182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001534315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inositol monophosphatase"
                     /protein_id="HGU4548115.1"
                     /translation="MIDAHDFTRWVRTQDTRLAPVLQGLFDLYIRGRDNRARTTKPEN
                     ADTLYFTVDDCYRVDFTPHGLALHCLTPHGESLLAYYDSPASIFAAMLAHRTAGGCAS
                     LSEYSAEFNRLSALFSQEWQRVTGYQP"
     gene            3560..6400
                     /locus_tag="ACHOSC_RS10915"
                     /old_locus_tag="pgaptmp_002183"
     CDS             3560..6400
                     /locus_tag="ACHOSC_RS10915"
                     /old_locus_tag="pgaptmp_002183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310660.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replication endonuclease"
                     /protein_id="HGU4548116.1"
                     /translation="MSAFAWSWNEPRPAIDPARFTERRQETETDLQRAIRYYLEANKR
                     AQEEQEAKEEAFFAQSTVGKKLMASLEEAGQREKLAQSIISKRQATEQDPVARAFATL
                     KVLPVYLREPLSRHLSFLRKKQEADRQKGKKSWQAERYARGTLRKIFERLDRTDSRWL
                     TPGYRSLAGRERLDDLLYLPQLNKHQIQTLATMTAAMFSGTFEKLCDGFGATDGELTM
                     DVTLKAYQMLARMALHLHAMPPHYDALTTDKDRRNEPDTELLPGAILRLTCAEWWKRK
                     LWLLRCEWREEQLRAACLVSRKTSPYLSQDALSEFRAQREKTRDFLKSFMLENEDGFT
                     IDLETVYYAGVSNPIHRKAEMMATMKGLELLAEARGDRAVFLTVTCPSKYHATTENGH
                     PNPKWNGATMRDSSDYLVNTFFAAVRKKLNRDGLRWYGIRTVEPHHDGTVHWHMMVFA
                     HPEEIDTIVSHTRDIAIQEDRHELGNDITPRFKVEYVDGSKGTPTSYIATYIGKNLDS
                     RAVDGIDPKTGKPRVDHETGKSMAESVERAIGWARLHRVRQFQFFGIPSRQVWRELRR
                     LASQMARNPEGPQRLKDDAMDAVLAAADAGCFATYIEKQGGVLVPRKDYLIRTAYDLA
                     DELNDYGEQSVQIYGIWSPLIGESSRVCTHPDNWKLVRRKPEAEDSTRENGFDLQGGP
                     AAPWTRGNNCPRVQETDNNGTEQPEERPAPWPQLPDGVDVNEWMRSLKRHERRALMRS
                     LRDKQAKNSSDEMQNWTQSRKQPRPLPDNHELLAKEWRESAESLGLHIGEQQMQHLLR
                     GGSLYVDGSIIAPQGFEIVRKPDTRPDSRITQLWQRLSRNHGVSSTEIRHNPVASYLE
                     QLGASDPEAAAHLASTLQQDQNTMKTPVTVLSDMLRAIRDAVHAQRISETTERAHRKA
                     DLLRGSLTSGNKKQTETGLTNPVNEQKTCRDI"
     gene            6477..7436
                     /locus_tag="ACHOSC_RS10920"
                     /old_locus_tag="pgaptmp_002184"
     CDS             6477..7436
                     /locus_tag="ACHOSC_RS10920"
                     /old_locus_tag="pgaptmp_002184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_process: GO:0030541 - plasmid partitioning [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid segregation protein ParM"
                     /protein_id="HGU4548117.1"
                     /translation="MKICIDDGSTNIKLAWTENGERRNAISPNSFKSEWSAPFGGTQP
                     ANYMLDGVRYGFDPVSDRFVQTTDTQYQYSDVNIIAIHHALVKSGITPQEVDVVVTLP
                     LSEYFDTNAQPDMANINRKKANVMRPVEYQNGEAFTIRNVRVMPESIPAGFKALADMS
                     PFESLLIVDLGGTTLDVAKVQGQLAGISQVFCDPHVGVSLMADAVLSVMATNGMRTSH
                     HIANTIIEHRHDEAWLRQHIHNDAHYASLMAVIREKEETLKQRVIRALAGFSGYGRVM
                     VVGGGAEIVAPAIREACGVNATFIADGVPQFALVNGLYAMDKE"
     gene            7441..7752
                     /locus_tag="ACHOSC_RS10925"
                     /old_locus_tag="pgaptmp_002185"
     CDS             7441..7752
                     /locus_tag="ACHOSC_RS10925"
                     /old_locus_tag="pgaptmp_002185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012907691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid partitioning/stability family protein"
                     /protein_id="HGU4548118.1"
                     /translation="MTTPTRRISFYLKPTAVKNEGEACAWLDSLTPEARKSGQRVAFL
                     AGLALLKMNPAEAYRLAAWAGDEALSVTQTRTERPASQPVSTAQITSQMAGNIRALFP
                     E"
     gene            7816..8406
                     /locus_tag="ACHOSC_RS10930"
                     /old_locus_tag="pgaptmp_002186"
     CDS             7816..8406
                     /locus_tag="ACHOSC_RS10930"
                     /old_locus_tag="pgaptmp_002186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001289969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ead/Ea22-like family protein"
                     /protein_id="HGU4548119.1"
                     /translation="MSEINYQALRERYSPVPVPKCPICGEEMSIQRISGAQVVYACSG
                     YGDDGDFKIGRTLADEHYEKSRVTVLDVGDPEVLALLDWLETKDNRIAELEKIATDYA
                     LKFQKAQDALKYAALLHSRTAQQTNNFAVSLPDISEYFINDVFQPLRYERDVERAIIK
                     AGGKALWQEKHEDRTHQSCDVNRGWFSPLTTDKNNT"
     gene            complement(8896..9942)
                     /locus_tag="ACHOSC_RS10935"
                     /old_locus_tag="pgaptmp_002187"
     CDS             complement(8896..9942)
                     /locus_tag="ACHOSC_RS10935"
                     /old_locus_tag="pgaptmp_002187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001706445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HGU4548120.1"
                     /translation="MSRKNKKVRMSSRIDLADALRKESSLSAFTFDGPYRLTGHDLLD
                     NMYCADNGRWYETPVDWYGLARAARQTSWHQSALYFKRNVLLGCYIPHPLLSRQDFSA
                     LALDWFVFGNAFLELRSNMLGEPLKLRHALAKYMRRGSDLESWWYVQDGKDAFQFRPG
                     KVCHLMNPDINQEIYGMPEYLGALLSASLSHSADMFRKLYYDNGSHAGCIIYIGAAQV
                     NRESMDSLKETLQGARGGGAFKNVLIHAPNGGKEGVQILPFQQITAKDEFMNVKAASR
                     DDVLAAHRVPPQLMGAMPGEKSAFGDVEKAARVYAINELMPVMEAMKHINDWLGEEVI
                     RFNPYALLDTQPTS"
     gene            complement(9942..11693)
                     /locus_tag="ACHOSC_RS10940"
                     /old_locus_tag="pgaptmp_002188"
     CDS             complement(9942..11693)
                     /locus_tag="ACHOSC_RS10940"
                     /old_locus_tag="pgaptmp_002188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001737206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase family protein"
                     /protein_id="HGU4548121.1"
                     /translation="MIQDAFVRQRARQLYWQGYPPAEISRLMGINPNTIYAWKKRDQW
                     DETPPVQRVTQSIDARLIQLTEKQNKTGGDFKEIDLLTRQLKKLHDGQPDATVTGKKG
                     RAKKLKNHFTPEQIAALREKIISRLEWHQRGWFDSLTLCREAGIRNRMILKSRQIGAT
                     WYFAQEALLMALRDDVAQPYQRNQIFLSASRRQAFQFKSIIQKAAAEVDVELKGGDKI
                     ILSNGAELHFLGTSAASAQSYTGNFYFDEFFWVSRFAELRKVAGAMATLSGLRRTYFS
                     TPSTETHEAYAYWNGDRWNEKKATHKRQRFSVDWKTLHNGLICPDRTWRQIVTLEDVV
                     NHGWKHTDIDEIRDENTEDEFLNLYMCEFVREGESAFNLNILIGCGVDGYDDWKDWKP
                     FAPRPMGNRPVWIGYDANGSSGNGDSGAVSVVVPPAVPGGRFRTVETRRVQGLEFEEQ
                     ARVIEEFTYRYNVEHIGIDVTGGNGEAVYQIVKRFFPAAIPYTFTLSSKRSLVLKMLQ
                     IMRAGRWEYDRAERELVAAFNAVRKVKTPGGFITYETDRARGISHGDLAWATMLAVIN
                     EPIGGEGENERFTVMEF"
     gene            11848..12684
                     /locus_tag="ACHOSC_RS10945"
                     /old_locus_tag="pgaptmp_002189"
     CDS             11848..12684
                     /locus_tag="ACHOSC_RS10945"
                     /old_locus_tag="pgaptmp_002189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001262634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0019069 - viral capsid assembly [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GPO family capsid scaffolding protein"
                     /protein_id="HGU4548122.1"
                     /translation="MANEKKTSRKKFRVAVSGSTVDGREISPVHLREAAENFNPDVYA
                     ARVNVEHYLSPCPSSEFSAMGDVTALSTEDITEGPLAGRTALYAEIEPTERMKQLIAD
                     GKKIYSSIELHPQFSVNGRAYLVGLAMTDTPASLGTERLKFTAQQRQAVMTFNSIQGE
                     APLISEAIESEIIEMAEQRQEEGTQWFNRVMGIIGRGRKADDASFSRIQEAVEGVATS
                     QVDIIDRFNVLETRHQQDRQKITSLTTELTALKEKLRTQDGDPQNRFTATGAASDQLA
                     DF"
     gene            12708..13760
                     /locus_tag="ACHOSC_RS10950"
                     /old_locus_tag="pgaptmp_002190"
     CDS             12708..13760
                     /locus_tag="ACHOSC_RS10950"
                     /old_locus_tag="pgaptmp_002190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020218748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019028 - viral capsid [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019069 - viral capsid assembly [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage major capsid protein, P2 family"
                     /protein_id="HGU4548123.1"
                     /translation="MNLVMSDITRNKLGCYMAQQASLNNIPVSALVSRFTVEPAVQQR
                     FENASKESTEFTKRINVIGVTDQKGEKILPDTTGPIARTNSSYDGIKRRNPNNVIDMK
                     SRQYQCEQVNYDTFISYPQLDTWAAHSDFQSRISTQIARQVALDRIMIGFNGTSHAYE
                     SDFHTNKLLQDVNVGWLEHIRTDASERVMNDVTLTSRNMDNTVAHAGKYANADALVQD
                     ARSSLLDEWHKEADDLVVIMGRNLFNSLRLPVLNSISGQNPNAELLAGQLILSSRTIG
                     GLGVFLAPFFPDATMLITSFNNLSIYWQKGSMRRLMKDEPEYNRIATYQSINDAYVVE
                     DYGKCAMVTGLKFADS"
     gene            13806..14606
                     /gene="gpM"
                     /locus_tag="ACHOSC_RS10955"
                     /old_locus_tag="pgaptmp_002191"
     CDS             13806..14606
                     /gene="gpM"
                     /locus_tag="ACHOSC_RS10955"
                     /old_locus_tag="pgaptmp_002191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000632360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA];
                     GO_process: GO:0019069 - viral capsid assembly [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage terminase small subunit"
                     /protein_id="HGU4548124.1"
                     /translation="MITPAQQHWQNVMAQRAGRANEGVDHAACTAHEEVLYRLRLAQA
                     RLKAIQARSAKAAIKKELLPDFSGWIEGTLEADGGQQDEVIATLMVWAIDCGDLPLAL
                     RIGAYVVRHNLIMPDNFGRTAATVLTEEICNPVLTQAGTDADADLSAFIEPLDTLWEI
                     VANQDIPDEVRAKLCKACAFARRGLTDADNMASSLKLLREAMHLNPNAGVKREIATLS
                     RALKKADSAAEPEDASAQQAQDESSKNKKTTRKPATRKTTATQKAKRG"
     gene            14708..15202
                     /locus_tag="ACHOSC_RS10960"
                     /old_locus_tag="pgaptmp_002192"
     CDS             14708..15202
                     /locus_tag="ACHOSC_RS10960"
                     /old_locus_tag="pgaptmp_002192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000063104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="head completion/stabilization protein"
                     /protein_id="HGU4548125.1"
                     /translation="MSMVARTNPGPAEDDITDTDDGDTRISAGAFWPDIVLRELRLAV
                     RLPGRVTTSRLLHTATGAIAHVTRELEAWQQEQQAAGYQTLADVPAPVINGESVNLWH
                     WRNAVYTATRALILERYRDADTTDKGDRRADALDIQTSDLWRDVSWAISDILGRPRMF
                     AELC"
     gene            15202..15402
                     /locus_tag="ACHOSC_RS10965"
                     /old_locus_tag="pgaptmp_002193"
     CDS             15202..15402
                     /locus_tag="ACHOSC_RS10965"
                     /old_locus_tag="pgaptmp_002193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000864894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tail protein X"
                     /protein_id="HGU4548126.1"
                     /translation="MKVKALEGDTVDSLCFRYYGTTRGVTEKVLDANPGLCQQVFLDA
                     GQEVEMPEPEKKKREMIQLWGE"
     gene            15405..15728
                     /locus_tag="ACHOSC_RS10970"
                     /old_locus_tag="pgaptmp_002194"
     CDS             15405..15728
                     /locus_tag="ACHOSC_RS10970"
                     /old_locus_tag="pgaptmp_002194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000104349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin family protein"
                     /protein_id="HGU4548127.1"
                     /translation="MSTIQTGITEQVIAWLFDHLPTVYAVGAAVSISALMSLYDGRTL
                     VQTVTGSLACGVLAMAVAGSLRFFGIPEDAVTFFGASIGFMGAEKARDKVIAIFDRRV
                     KERNE"
     gene            15725..16117
                     /locus_tag="ACHOSC_RS10975"
                     /old_locus_tag="pgaptmp_002195"
     CDS             15725..16117
                     /locus_tag="ACHOSC_RS10975"
                     /old_locus_tag="pgaptmp_002195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000072320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M15 family metallopeptidase"
                     /protein_id="HGU4548128.1"
                     /translation="MSNTFKFSSRSEKNLQGVNPDLVKVTRRALEISEVDFGITEGLR
                     SRYRQKQLVTTGKSQTMNSRHLTGHAVDVVAYVGSQVSWEWPLYEKIAAAFRQASREL
                     NIPVEWGGDWKTLKDGPHFQLPHGAYPA"
     gene            16114..16521
                     /locus_tag="ACHOSC_RS10980"
                     /old_locus_tag="pgaptmp_002196"
     CDS             16114..16521
                     /locus_tag="ACHOSC_RS10980"
                     /old_locus_tag="pgaptmp_002196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000780564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2570 domain-containing protein"
                     /protein_id="HGU4548129.1"
                     /translation="MKLWPTLGVAFLLIAAWGTSMRLSWSLGRENARNEAQASTLKST
                     VDTLNIISAGVQDMQQVLAQLRADNQQRNQDGEVRREQLRNDIAKDECAHALPDARFT
                     DRLRRHAERATASAVSPAYTADADHAGNASPLP"
     gene            16660..>17839
                     /locus_tag="ACHOSC_RS10985"
                     /old_locus_tag="pgaptmp_002197"
     CDS             16660..>17839
                     /locus_tag="ACHOSC_RS10985"
                     /old_locus_tag="pgaptmp_002197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sialate O-acetylesterase"
                     /protein_id="HGU4548130.1"
                     /translation="MTSKNFALITAMTQAELTQKVNEHLAKGWHLQGETRVAYEPGTP
                     WYLMQAMVADGTTDISPDSPQHGSVPEWYYVVVLAGQSNAMSYGEGMPLPDSYDAPHP
                     RIKQLARRNTVTPGGKACAFNDIIPADHCLHDVQDMSVLNHPNADLSKGQYGCVGQGL
                     HIAKRLLPYIPQNAGILLVPCCRGGSAFTQGAEGTFSESTGASQDSARWGVGKPLYQD
                     LILRTKAALQKNPKNMLLAVCWMQGEFDMSAATYSQQPPLFAAMLKQFRADITEFNTQ
                     CHGGRAASVPWICGDTTYYWKNTYGTQYDTIYGAYKNRESDNVFFVPFLTDGSGNNTS
                     TNAPTEDPDAASEGYYGSASRTNKNWVSSNRQTHFSSWARRSIIPDRMATAILNVAGR
                     T"
BASE COUNT         4609 a         4815 c         4621 g         3794 t
ORIGIN      
        1 ccaaaacccc tacgcagata aagaatatta tttcttttgg ataaagtcgg attaattctg
       61 aagcacgaag tcgaacttct ggtaaggccg aactctctgt gtgacttgat gccgattgtt
      121 gctctaacca cgacaatgca gactgtaact gagaacgagt aagatcgttt aaacgtcctg
      181 taccgaaatt gatatggcaa taccgcagaa gtttttgtcg aagtccacag tcttcactgt
      241 tacgtagtaa taaacttaca agagtcttac aggcatcatg atctttacat cgctcaagca
      301 ttgcatgcag aaaactctcc gctgttttat attgatttac tgtcatatca tcaataccag
      361 ctacaccaat ctccgcatgt actttttgcc aaataataaa cgcttcagta ttgctagctt
      421 ctgcaatagc agcaaccaag ctatttagct ccttacgctg agccttaagc aaagggcgat
      481 cgtcatcatc attattcgaa gggattgcga tattgacggt atgagaacca tcatatcgct
      541 ctatctgaat attcttttcg tgaaaatcac gcccagcaac gcgattgttt gaaccgtttg
      601 agttgacggc catgtcacct ccctactatc acctaccctt agtttcgtta tagtcacgac
      661 cagcgatacg gttattacca ccagaaatat ttaactcacg tcctgatggc tgagtttcct
      721 tttcactgat cgcacctttt aaagccccga tcaccgcgtt tttcacatct aacgaagctg
      781 ctcgaaagcg agtaatcaac tcctgctcat catcgttata agtttcaggt gagtgaattc
      841 ccaacacaac atactgaaca tcaaggccaa aacgagacag cgctgccaaa tacgcagcat
      901 caggaaagct atctcctttc tcatatctaa gctgagttag ctttttgact ccaccaatgt
      961 cgctcatggc aacttgacta agtcccaatc tttccctttc ctcacgcaac cgctgaccaa
     1021 tatcattttt catacaaaaa ccttgacagg tatctttttt gataccaaaa tgatttcacg
     1081 agctattaga tgatcacaat ataccactat gaaacaagtt cttcacgata ccagatcacg
     1141 cattccgcgt aacaccgcca caggtccaag actggcactt cggctgtccc tcgaggagcg
     1201 agccgtcatt gatgaaatgg cagctaaaga acaacgctca tcctctaaca tggcgcgcat
     1261 gatcttcctt cgcggcctag agctaaccca gaaagaacaa aacaaatctt cctgatcagg
     1321 aggctagtgg gatgtcaggt ataaccatca atatcaatgt gaatgccccc tatgtatccc
     1381 tgcagaaata tgcagagata acaggtatcc ctcttaatac atgcaaaaag atgttggctg
     1441 acggtcgaat tattatccgc cccaaacgcg ccaaaatgga aaagcctgaa gtaaaccttg
     1501 tggcgatgtt aaaagacgct ttggctaaca gctaaaacaa tgaacagagc atcatcatga
     1561 aaaaaaacac taataatcca tactccaaat ttcgtaatgg cgtagaacgc catgtacacc
     1621 acgtcgctac cagtgcatca cgtagtaaca gccgctataa cctgaacgag acgcacgcaa
     1681 caccagatgg gcacgctgta aaacaaatcg gcgaacacac ctggctgatt gagaaagctg
     1741 gaatcgtggt tcacagatgc ccacacaatc cgtttaccgg aaaccgcatt tttgcattaa
     1801 gcagcggcga taatcagttc gggcaggatt tcacattgta cgaagcacta cgcacagtcg
     1861 atcgtctgct tcgcgggcaa agttttatta aacaggctgc tttataacag gtgctttatg
     1921 actaaagacc atgcacaagg tgtatttatc cgctttattg attttcgcgg tgaactgtta
     1981 ttacgcgcat cagctattga cggagtagtt ccatctgaaa aaaacgcagc tacctacgtt
     2041 tatctgaacg gtacacgcct gactgtagag cttccgtacc agatcattcg cgaaataatt
     2101 agcgaagccg aaaaatctcg tcaggttaat ggcgatgaac cctatatcga gattatctgc
     2161 atggattcag aagctgaaat tcagaaagca gattaaaggg tgttgcgatg gataaagaat
     2221 ataaaactct catcaacaaa gcacttgagc gtttttattt tcgtttaagc gcatccggcg
     2281 ctcattcaga acgtgcagcc cgtgactcat tgaccagggc tatccggagt ctgtatgacg
     2341 tagcttttta cgctgacgat ctggatgcac ttaatgaact ttctgagctt atctgcgccg
     2401 cagaatgcgg ggagcatatt gagccgtaca aactgggaaa tatcgcatga gtatatttat
     2461 atcctggatt gttcttatta tttcggtggt ttgtgcaatt ggtattatgc gaattattaa
     2521 ttcagtaaaa aagattgaac gctttttcac tggcgaataa ccgcataaat aaaaccccag
     2581 cttaaataaa aaaatgtgaa cacaatctgc attcgcagag gtattcacac acaccaagga
     2641 ggcgtaatgg caattaagca ttttcctgtc gttcgtttca cctccagagg acgtgaatac
     2701 gaagtcgacg aacgtctgat taccacaatc gacaaacacc gctcagaaaa ggatgcacac
     2761 cacatctatc tcactgacgg cacttacttt tgcgccacta atgtggcgcg ggtgaatctt
     2821 atccgacagg tacaggatcc acgcaaatga gtaggagaag aatcactcgc agacatcacc
     2881 gaacacacct gagttcctca gcaacgctaa aggcacttat tcaaagcgag atcggtgatt
     2941 tcttcgcggg agttggctca ccaggcgaac cagaaacacc agaagcgatg cagcgtgagc
     3001 tcatgatacg catagataac acttttgatt tcttctacag catgcacgga attaaacaga
     3061 aatgaacctc aagccagcaa taactactcg tagaacgtca attcctgtaa ccgaccgctt
     3121 ctgagttttt tggcaggaag ccttcgcaca tctgtagtaa agagaattgc agcatgattg
     3181 acgctcatga cttcacaaga tgggtgcgca cacaggacac ccgtctggct cccgttcttc
     3241 agggattatt tgatctctac atccgtggtc gtgacaacag agcacgcacc acaaaaccgg
     3301 agaatgcaga caccctttat ttcacagtag acgactgcta ccgcgtggac ttcacaccac
     3361 acgggctggc gttgcactgc ctgacaccac acggcgaatc actgctggcg tattacgact
     3421 ccccggcctc catatttgcg gcaatgctgg cgcatcgcac tgctggcggg tgtgcctcgc
     3481 tgagtgaata ctccgctgaa tttaaccgcc tttccgccct cttctcacag gagtggcagc
     3541 gcgtgacggg ataccagcca tgagtgcgtt tgcatggagc tggaatgaac cacggccagc
     3601 cattgatccg gccagattta cggagcgcag gcaggaaact gaaaccgacc tgcaacgcgc
     3661 catccgttac taccttgagg caaacaaaag ggcacaggaa gaacaggaag cgaaggaaga
     3721 agcctttttc gcacaatcca ccgtgggtaa aaaactcatg gcatcccttg aggaagccgg
     3781 acagcgtgaa aagctggcac aaagcatcat cagcaagcgt caggcaacag aacaagaccc
     3841 ggtggcccgt gcttttgcca cactgaaggt gcttcccgtt tatctgcgtg aacctctgag
     3901 ccgccacctc tcttttctgc gcaagaagca ggaagccgat cgtcagaaag gcaaaaagag
     3961 ctggcaggct gaacgctacg cgcgcggaac cctgcgcaaa atattcgaac gtctggaccg
     4021 caccgacagc cgctggctga caccgggcta tcgctccctt gccggacgcg aacgcctgga
     4081 cgatttgctt tacctgccgc agctcaacaa acaccagata cagacgctgg ccaccatgac
     4141 ggcggcgatg ttcagtggca cttttgaaaa actctgcgat ggctttggcg cgaccgatgg
     4201 cgagctgacc atggatgtaa cgctgaaggc gtatcagatg ctggcccgca tggcgttaca
     4261 cctgcacgct atgcctccac attatgacgc tctgacaaca gacaaagacc ggaggaatga
     4321 accggacacg gagctgctgc cgggcgcaat ccttcgcctg acctgtgcgg aatggtggaa
     4381 acgcaaactg tggctgttac gttgcgagtg gagagaagaa caactccgcg ccgcctgtct
     4441 ggtttccaga aaaacatcac cctatctgag ccaggacgcg ttaagcgagt ttcgcgcaca
     4501 gcgcgagaaa acacgcgatt tcctgaaaag tttcatgctg gaaaacgaag acgggttcac
     4561 gattgatctc gagacagtgt attacgcggg agtaagtaac ccgattcacc gtaaggcaga
     4621 aatgatggcc accatgaagg ggctggaact tctggccgaa gcccgtggcg acagagcggt
     4681 gtttctgact gtcacctgcc cgtcaaaata ccacgccaca acagagaacg gtcatccgaa
     4741 tcccaaatgg aacggggcca ccatgcgcga ctccagcgat tacctggtta acacgttttt
     4801 tgcggcggtc cgcaagaaac tgaaccgcga cggcctgcgc tggtatggca tccgcacggt
     4861 ggagcctcac catgacggca ccgtgcactg gcatatgatg gtctttgctc atccggaaga
     4921 aatcgacacc attgtgtccc acacccgcga tattgccatt caggaagacc gccacgagct
     4981 gggcaatgat attactccgc gctttaaggt ggagtatgtc gacggctcaa aaggcacgcc
     5041 aaccagctac atcgccacct acatcggaaa gaacctggac agccgcgccg tggatggcat
     5101 cgacccgaaa acgggcaagc cacgcgttga ccacgaaacc ggaaaatcaa tggccgagag
     5161 cgtggaacgc gccatcggct gggcacgcct tcaccgggtc cgccagttcc agttctttgg
     5221 catcccctcc cgtcaggtat ggcgtgaact ccgccgcctt gccagccaga tggcacgcaa
     5281 cccggaaggc ccacaacggc tgaaggatga tgcaatggat gcggtactcg ctgccgctga
     5341 tgccgggtgt tttgccacct acattgagaa acagggcggc gtacttgttc cacgcaaaga
     5401 ctacctgatt cgcaccgcct acgacctcgc agatgagctg aacgattacg gcgaacagag
     5461 cgtacagatt tacgggatct ggtcaccact catcggggaa tcctcccgtg tgtgcacgca
     5521 cccggataac tggaagctgg taagacgtaa accggaagcg gaagacagca cccgcgaaaa
     5581 tggttttgac cttcagggcg gccctgccgc cccttggact cgtggcaata actgtccccg
     5641 tgtacaggaa acggacaaca acgggacaga acagccggaa gaaaggccag caccgtggcc
     5701 gcagcttcct gacggcgttg acgtgaatga atggatgcgc tcactgaaac ggcacgaacg
     5761 ccgggcgctg atgcgttcgc tgcgtgacaa acaggcaaaa aacagcagtg atgaaatgca
     5821 gaactggaca cagagccgca aacagccacg gcctttgcct gataatcacg agttactcgc
     5881 taaagaatgg cgggagtctg ctgaatctct cggcctgcat atcggtgaac agcagatgca
     5941 gcacctgtta cggggcggca gtctgtacgt tgacggcagc atcattgcac cgcagggatt
     6001 tgaaattgta cgcaaaccag atacccgccc ggacagccga atcacgcaac tctggcagcg
     6061 cctgagccgt aatcacggcg taagcagcac ggagatccgc cataacccgg tcgccagcta
     6121 tctggaacag ctgggggcat cagaccccga agccgccgca cacctggcat ccacacttca
     6181 gcaggaccag aacaccatga aaacccccgt taccgtgctt tctgacatgc tgcgcgccat
     6241 ccgtgacgca gtgcacgcac agagaatcag tgaaaccact gaacgcgccc accgcaaagc
     6301 agacctgctg cggggtagcc tgaccagtgg aaacaaaaaa cagacagaaa cgggactcac
     6361 aaatcccgta aatgagcaaa aaacgtgccg cgatatatga agcgcgcaca aaacaggcaa
     6421 aagcgggatt taaaaatcct gtaaccgatt aattaatcaa cacaaggaaa agcgacatga
     6481 aaatttgtat cgacgacggc tccaccaaca tcaagctggc atggactgag aacggcgaac
     6541 gccgcaacgc catcagcccg aacagcttca agtcggaatg gtctgcgccg ttcggtggca
     6601 cgcagcccgc gaactacatg cttgatggcg tgcgctatgg ttttgatccg gtcagcgatc
     6661 gctttgtcca gacgaccgac acgcaatacc agtacagcga tgtgaatata attgccattc
     6721 atcacgcgct ggtcaaatca ggcatcacac cacaggaagt ggatgtggtt gtcaccctgc
     6781 cactgagcga gtatttcgat acaaacgcac agccggacat ggccaacatc aaccgcaaaa
     6841 aagcgaacgt catgcgcccg gtggagtacc agaacggcga agcattcact atccgtaacg
     6901 tacgggttat gcctgaatcc attccggctg gctttaaggc actggctgac atgagtccgt
     6961 ttgaatccct gctgattgtg gatttgggcg gaaccactct ggatgtggca aaggttcagg
     7021 ggcaactggc aggtatcagc caggtgtttt gcgatccaca cgtgggcgtt tctctgatgg
     7081 ccgatgccgt actgtcggtg atggccacta acggtatgcg caccagtcac cacatcgcca
     7141 ataccattat cgaacatcgc catgatgaag cctggctgcg ccagcacatc cacaatgacg
     7201 cgcattacgc cagcctgatg gcggttattc gtgaaaagga agaaacactg aaacaacgcg
     7261 tgatccgcgc gctggcgggt ttttcgggtt acgggcgggt gatggttgtc ggtggagggg
     7321 cggagattgt ggcacccgct atccgcgaag cctgcggagt taatgcgact ttcatcgcgg
     7381 acggggtgcc acagtttgct ctggttaatg ggctgtacgc aatggacaag gagtaaacca
     7441 atgacgactc caaccagacg gataagtttc tatctgaagc ccaccgccgt caagaacgaa
     7501 ggagaagcat gcgcctggct ggacagcctt acaccagaag cccgcaaaag cggccaacgc
     7561 gtggcttttc tggccgggct ggcacttctg aaaatgaatc cggcagaggc ttatcgactg
     7621 gctgcatggg ctggtgatga agcgttatca gtgacacaaa ccaggacaga acgccccgca
     7681 tcacagccag tatcaaccgc acagataacc agtcagatgg ccggaaatat ccgggcgtta
     7741 tttcctgaat aacacaacat cagggcgagt tcgccctgct ctccacctca gaacataaac
     7801 aaggagaacg acttaatgag cgaaatcaac tatcaggcat tacgtgaacg ttattcacct
     7861 gtgccagtac cgaaatgccc tatttgcggc gaggaaatgt caattcagcg aatatctgga
     7921 gcacaggttg tttatgcctg ctccggttat ggtgatgatg gagatttcaa aattggccga
     7981 actcttgccg acgaacatta tgaaaaatca cgcgtaacag tgttggatgt cggggatcct
     8041 gaagtattgg cgctacttga ttggctggaa accaaagaca accgaatcgc tgaactggaa
     8101 aaaatcgcca ctgactatgc acttaaattc cagaaagcac aggacgcatt aaagtatgcc
     8161 gctttgctgc atagcaggac ggcgcaacaa acaaataatt ttgcagtatc gcttccggac
     8221 ataagcgaat atttcattaa tgacgtattt cagcccttgc gatacgagcg ggatgttgaa
     8281 agagccatca taaaggctgg cggaaaagca ttgtggcagg agaaacacga ggacagaacg
     8341 catcagtcct gcgatgtaaa tcgtggatgg tttagcccac tgacgacaga taaaaataac
     8401 acctgatccc cctcaaacca gggcgataat cgcgcatcgc cctgctgcac aatagtgcac
     8461 aagtttgcac aatttttttg aacgactttt tacccttccg gcccgcatgg cggctggatc
     8521 cgtcaaggat ccgtgcgtgc acaaaaaaac gcgctttttc tgcgcgcagg tgacggggga
     8581 acagcccgcg tttcaggggg taaatagcat cccctgaacg atgtcgcagc gacacaacag
     8641 aatggctgta tttctcacgc tgagcgtgaa aaagacgtga gggcttttga tttgatgggt
     8701 ggcagataag gccgtcaaaa tcgcactgag gcggcgagaa catgcagtca acgcggtggg
     8761 attgcgtaag agtctgaccg ccgatgatga caataagcag aaaagcgtcg tgaaattatc
     8821 tgattgatac aggagctgga gagtcggggc ataaattttt tatgccccgg cgaagcagca
     8881 gacaagcgaa gcgcgtcagg atgtgggctg ggtatctagc agtgcgtaag ggttaaagcg
     8941 gatcacctct tcgccaagcc agtcattgat gtgcttcatg gcctccataa cgggcatcag
     9001 ctcgttaatt gcgtaaaccc gcgcggcctt ctccacatca ccaaacgcac ttttttcgcc
     9061 cggcatcgcc cccatcagtt gcggcggaac gcggtgcgca gccagcacat catcacggga
     9121 tgccgcctta acattcatga actcatcctt tgcagtgatc tgctggaacg gcaaaatttg
     9181 cacaccttct ttgcccccgt tgggcgcatg gatgagtacg tttttaaacg caccaccacc
     9241 acgcgcgccc tgtaacgttt ctttcaggga gtccatgctt tcgcggttta cctgcgctgc
     9301 accgatgtag atgatgcacc cggcgtggga tccgttgtcg tagtacagtt ttctgaacat
     9361 gtccgccgaa tgagaaaggc tggccgagag taatgcgcca agatattccg gcatgccgta
     9421 gatttcctgg ttaatatccg gattcatcag gtggcacact ttgccagggc gaaactggaa
     9481 cgcgtccttg ccatcctgca cataccacca tgattcaaga tcgcttccgc gtcgcatgta
     9541 tttcgccagg gcgtgccgta atttaagcgg ttcgccgagc atattacttc taagctcaag
     9601 gaatgcgtta ccgaacacaa accagtccag cgccagcgcc gagaaatcct gccgggaaag
     9661 cagcgggtgc gggatgtagc aaccgagtaa tacattgcgc ttaaagtaaa gcgcagactg
     9721 atgccaggac gtttgccggg ctgctcttgc cagaccgtac cagtccaccg gggtttcata
     9781 ccaccgtccg ttatcagcac agtacatatt gtccagcagg tcatgcccgg tcaggcgata
     9841 aggaccatca aatgtgaatg cactgagcga tgattctttc ctgagcgcat cagcgagatc
     9901 aatgcgtgaa ctcatgcgca cttttttatt ttttctgctc atcagaactc cataaccgtg
     9961 aaacgctcgt tttctccttc gccgccaatc ggttcgttaa tgacagcaag catggttgcc
    10021 cacgcaaggt cgccgtggct gatccccctc gcgcggtccg tttcgtaagt gataaagccg
    10081 cccggtgttt tcaccttacg cacggcgtta aaggccgcga ccagctcgcg ttcggcgcga
    10141 tcgtattccc accgcccggc acgcattatt tgcagcattt tcagtaccag cgaccgtttt
    10201 gatgacagcg tgaaggtgta cggaatagca gcagggaaaa accgtttcac tatctgataa
    10261 acagcctccc cgttcccgcc cgtcacatca atgccgatgt gttccacgtt gtagcgatac
    10321 gtgaactctt caatgactct ggcctgttct tcaaactcca gcccctgaac gcgtcgcgtc
    10381 tccaccgttc gaaaacggcc accaggaaca gccggaggaa ccaccacgga cacagcgccg
    10441 ctgtcgccgt tgccactact gccgtttgcg tcatacccaa tccataccgg acgattcccc
    10501 atcgggcggg gagcaaaagg tttccagtct ttccagtcgt cgtatccgtc aacaccgcag
    10561 ccaatcagga tattcaggtt aaatgccgat tccccttcgc ggacaaactc acacatatag
    10621 agattgagga actcgtcttc ggtgttttca tcacgaattt cgtcgatatc ggtgtgtttc
    10681 cagccgtgat tgaccacatc ttccagcgtg acaatttgcc gccacgtccg gtcagggcag
    10741 ataagcccgt tatgcagcgt tttccagtcc acagaaaaac gctggcgttt atgcgtggcc
    10801 tttttctcgt tccagcggtc gccgttccag taggcgtatg cctcgtgcgt ttcggtggat
    10861 ggcgtggaga agtaggtgcg ccgcagtccg ctgagggttg ccatagcgcc agccaccttg
    10921 cgcagttcag caaagcgact gacccagaaa aattcatcaa aataaaaatt gcccgtatag
    10981 gactgtgccg acgcagcaga agtgccgaga aaatgcagct ctgcgccgtt ggagaggatg
    11041 attttatcgc cccctttcag ctccacatca acttcagccg cggccttctg aataatgctt
    11101 ttaaactgga acgcctgacg acgcgacgca gacaaaaaaa tctggttacg ctggtaaggt
    11161 tgcgccacat cgtcacgcag cgccatcagc agagcttcct gtgcaaaata ccaggtcgcc
    11221 ccaatctgtc gggatttcag gatcatcctg ttacgtatcc cggcttccct gcaaagggtc
    11281 agggagtcaa accagccccg ctgatgccac tccagcctgc tgatgatttt ttcccgcagt
    11341 gcggcaatct gttccggcgt gaaatgattt ttgagttttt tcgcccggcc tttctttcct
    11401 gtgaccgtcg catccggctg gccatcatgc agttttttaa gctgccgggt cagcaggtct
    11461 atttccttaa agtcaccgcc tgttttattc tgtttttcag taagctggat gaggcgcgca
    11521 tcgatggact gcgtgacacg ctgcacgggt ggcgtttcat cccactggtc gcgttttttc
    11581 cacgcataaa tcgtgttcgg gtttattccc atcagacgtg atatttctgc gggcggataa
    11641 ccctgccagt aaagttgccg cgcacgctgg cgcacaaaag cgtcctgaat cattgctccc
    11701 cctgagtaat tacaggaaga ttacccgcgc gcgaaactgt tctccttaac cccctgttct
    11761 ggccgttttc ttacaacaaa atccctttgt atcagcctgt tacgctttgc catcatgact
    11821 gaagaaccag tcagaggggc aaaaactatg gctaatgaaa aaaagacatc ccgcaaaaag
    11881 tttcgcgtgg ctgtctccgg atcaactgtt gatggccgcg aaatcagccc ggtacatctg
    11941 cgtgaagccg ccgagaactt caacccggat gtttacgctg cccgcgtgaa cgttgagcac
    12001 tatctctcgc catgcccgtc aagcgaattt tccgcaatgg gcgatgtcac cgcgctgagt
    12061 acggaagata ttacggaagg cccgctggcc ggacgtactg cgctgtatgc agaaatcgaa
    12121 ccgaccgagc gcatgaagca gcttatcgct gacggcaaga aaatctattc cagtatcgaa
    12181 ctgcacccgc agttctccgt taacgggcgt gcctatctgg tcgggctggc gatgaccgac
    12241 accccggcaa gcctgggcac tgagcgcctg aaattcacgg cacagcagcg tcaggcggtg
    12301 atgacgttca acagtatcca gggtgaagca ccgcttatct ccgaagccat cgagtctgaa
    12361 atcatcgaaa tggcagaaca acgccaggaa gaaggcaccc agtggtttaa ccgcgtaatg
    12421 gggattattg gtcgtggccg caaagcggat gacgccagtt tctcccgtat tcaggaagcg
    12481 gtggaaggcg ttgcaacatc acaggtcgac attatcgacc gttttaatgt gctggaaacc
    12541 cgccatcagc aggaccgcca gaaaatcacg tcactgacca cagagctgac agcactgaag
    12601 gaaaaactgc gcacgcagga cggcgatccg cagaaccgct tcaccgcaac gggcgcagcc
    12661 tccgaccagc tggctgactt ctgataagac aaaggagcaa attttttatg aatctggtga
    12721 tgtcagatat tacccgcaac aagctgggtt gctatatggc gcagcaggcg tcgcttaaca
    12781 atatcccggt atctgcactg gtatcgcgat ttaccgtgga acccgcggtg cagcagcgtt
    12841 ttgaaaacgc ctcaaaggaa agtaccgaat ttacgaaaag aattaacgtg atcggcgtga
    12901 ccgaccagaa aggcgaaaaa atcctcccgg acaccaccgg gccaattgcg cgcacgaata
    12961 gcagttatga cggcatcaaa cgccgtaacc cgaataacgt gatcgatatg aagtctcgtc
    13021 agtaccagtg cgaacaggtg aactacgata cctttatttc gtacccacag cttgatacct
    13081 gggcggccca cagcgatttt cagtcccgta tcagtacaca gatcgcccgg caggtagcgc
    13141 ttgatcgcat catgattggc tttaacggca catcccacgc ctacgagtct gattttcaca
    13201 ccaacaagct gcttcaggac gttaacgtgg gctggctgga gcacatcaga accgatgcca
    13261 gcgagcgcgt aatgaatgac gtgacgctga cctcccgcaa catggacaac actgtggcgc
    13321 acgcgggtaa gtatgcgaat gccgatgctc tggtacagga tgcgcgctca tccctgctgg
    13381 atgaatggca caaggaagct gacgacctcg tggtgattat ggggcgcaac ctgtttaact
    13441 cgctgcgtct gcccgtgctg aacagcatca gcggccagaa tcccaatgcg gaattacttg
    13501 ccgggcagct catcctgtca tcgcgcacca ttggcgggct gggcgtgttc cttgcgccgt
    13561 tcttcccgga tgcaacgatg cttatcacct cgttcaacaa cctgtcgatt tactggcaga
    13621 aaggttcaat gcgtcgtctg atgaaagacg agccggaata caaccgcatc gccacctacc
    13681 agtccatcaa tgacgcttat gtcgttgaag actatggcaa gtgcgcgatg gtcactggcc
    13741 tgaagttcgc cgacagctaa tcaactcacg gcgggcatca tgcccgcctg taacggagag
    13801 aaaaaatgat tactcctgca caacaacact ggcagaacgt gatggcacag cgcgcaggcc
    13861 gggcgaatga aggtgtggac cacgccgcgt gtaccgcgca tgaagaagtg ctgtatcgtc
    13921 tgcgtctggc acaggccagg ctaaaggcca tacaggccag aagcgcgaaa gccgccatca
    13981 aaaaagagtt gttgccggac ttttccggct ggattgaggg aacgctggag gctgacggcg
    14041 ggcaacagga cgaagtaatt gccacgctga tggtgtgggc gattgactgc ggcgatcttc
    14101 cgctggcgct gcgtattggt gcatatgtgg tccgtcacaa cctcatcatg ccggataact
    14161 ttggccgtac tgctgccacg gtactgaccg aagaaatctg caacccggta ctgacgcagg
    14221 ctgggacgga tgccgacgcg gatttatccg cctttatcga accactggac acactttggg
    14281 aaattgtcgc caaccaggac attccggacg aagtgcgcgc caaattatgc aaggcgtgtg
    14341 cctttgcccg tcgtggcctg accgatgcag acaacatggc ctcatcactg aagctgctgc
    14401 gcgaagcgat gcacctgaac ccgaacgcag gtgtgaaacg cgagattgca accctttccc
    14461 gcgccctgaa aaaagccgat tccgcagccg aaccagaaga cgccagcgca cagcaggcgc
    14521 aggacgaaag cagcaaaaat aaaaagacaa cgcggaagcc tgcaacacga aaaaccaccg
    14581 cgacgcagaa ggcaaagcgc ggttaacgac tgaccccgtc agcgggcggc gtgcgcggtg
    14641 ttccggtttg actccgtgac cgtttacacc gcgcacccac cgcccgattt tttcaggagt
    14701 gaaccccatg agtatggttg ccagaactaa ccccggcccc gcagaggacg acatcaccga
    14761 taccgatgat ggcgacactc gcatttcagc gggtgcattc tggccggata ttgtgctgcg
    14821 tgagctgcgt ctggcggtac gactgccggg ccgcgtgacc acctcccgcc tgctgcatac
    14881 cgccaccggg gccatagcac acgttacccg cgagctggaa gcatggcagc aggaacaaca
    14941 ggcggctggc tatcagacgc tggccgatgt tccggcccct gtaattaacg gagaaagcgt
    15001 caatctctgg cactggcgca atgctgttta taccgccaca cgcgccctga ttctggagcg
    15061 ttaccgcgat gcggacacaa cggacaaggg cgaccgccgg gcggacgcac tggatataca
    15121 gacatcggat ttgtggcgcg atgtgagctg ggccatctct gacattctgg gacgaccgcg
    15181 aatgtttgcg gagctgtgct gatgaaagtg aaggcactgg aaggcgacac cgtggattcg
    15241 ctctgtttcc ggtactacgg cacgacacgg ggcgtcaccg aaaaggtgct ggatgccaac
    15301 cccggactct gtcagcaggt atttctggac gccgggcagg aagtggagat gccggagccg
    15361 gagaagaaga aacgagaaat gattcagttg tggggggagt agcagtgagc accattcaaa
    15421 cagggatcac agagcaggtt attgcgtggc tctttgacca cctgccaacg gtgtatgcag
    15481 taggcgcggc tgtcagcatt tccgcgctga tgagtcttta tgacggacga acactggttc
    15541 agaccgtaac gggatcgctg gcgtgcggcg ttcttgccat ggccgtggcc gggtcgctgc
    15601 gcttcttcgg gatccctgag gatgcagtga cgttttttgg tgcctcaatc ggttttatgg
    15661 gcgcagagaa agcacgcgac aaggttattg caatatttga tcgcagggtg aaggagagga
    15721 acgaatgagc aacacattta aattcagcag ccgaagcgaa aagaatttgc agggtgtaaa
    15781 tcctgatctg gtgaaagtga cccgacgggc gctggaaatc tcggaagtgg attttggtat
    15841 caccgaagga ttgcgcagtc gttatcgtca gaagcagctc gtgaccacag gcaagagcca
    15901 gaccatgaac agtcgccatc tcacagggca tgccgtggat gttgtggctt atgttggcag
    15961 ccaggtgtca tgggaatggc cgctgtacga aaaaatcgca gcagcattca gacaggccag
    16021 ccgggaactg aatattccgg tggaatgggg cggcgactgg aagaccctga aagacggacc
    16081 gcattttcag ttaccacacg gagcctatcc ggcatgaagc tctggcccac gcttggcgtc
    16141 gctttccttc tgattgccgc atggggaaca tccatgcgtc tgtcgtggtc gctgggccgg
    16201 gagaacgcca gaaacgaagc gcaggccagc accctgaaaa gtaccgtcga cacactgaat
    16261 atcatcagcg ccggggtaca ggatatgcag caggtgctgg ctcaactccg cgcggacaat
    16321 cagcaacgca atcaggacgg agaggtaaga cgtgaacagc tacgcaacga tattgcaaaa
    16381 gatgaatgcg cccacgcttt gcctgacgct cgttttactg acaggttgcg caggcacgca
    16441 gaacgcgcca cggccagcgc cgtcagtccg gcttataccg cagacgctga ccatgccggt
    16501 aacgcctccc cccttccctg accaacccac atggggaaac ctcggaatat ggggcgaccg
    16561 ccttctggat gcactggaaa cctgtaacgc ggataaacgg gccattgctg aactggataa
    16621 gagaatagcc gaactgacac accagacggg agtaacacaa tgaccagtaa gaactttgca
    16681 ctgattacag ccatgacaca ggctgaactg actcaaaagg tgaatgaaca tcttgcgaaa
    16741 gggtggcatc ttcaggggga gacgcgggtt gcctacgaac ccggcacccc gtggtatcta
    16801 atgcaggcaa tggtggccga tggcactaca gacatctcac ctgattcccc ccagcacggc
    16861 agcgtgccgg agtggtatta cgtggtggta cttgctggtc aatccaatgc catgtcatat
    16921 ggtgagggaa tgccgctgcc ggattcttac gatgcgcccc acccacgcat taagcaactg
    16981 gcccgtcgca acacagtgac tcccggtggt aaagcatgcg catttaacga catcattccg
    17041 gcagatcact gcctgcatga tgttcaggat atgagcgtac tgaatcaccc gaatgcagac
    17101 ctgagcaaag ggcagtatgg ctgtgtcgga cagggcttgc atattgccaa acgcctgttg
    17161 ccttacattc cacagaatgc cgggatttta ctggttccat gctgtcgtgg tggttcggca
    17221 ttcacccagg gcgcggaggg gacattcagc gagtccacag gagccagtca ggattcggct
    17281 cgctggggtg tgggtaaacc tttatatcag gacctgattt tgcgcacgaa ggccgcattg
    17341 cagaaaaacc caaaaaacat gctgctggcc gtatgctgga tgcagggcga atttgacatg
    17401 agcgccgcta cgtactcaca gcaacctccg ctgtttgcgg ccatgctgaa acagtttcgt
    17461 gcggacatta ccgagtttaa cacgcagtgt catggaggca gagcggcaag tgtgccatgg
    17521 atttgtggtg acacgacgta ttactggaaa aacacctacg gcacgcagta cgacaccatt
    17581 tacggggcgt acaaaaacag ggagagcgac aacgttttct ttgtgccatt cctgaccgat
    17641 ggtagtggca acaatacctc caccaacgca ccaacggaag atccggatgc tgcaagtgag
    17701 ggatattacg gttcggcatc ccgaacgaac aaaaactggg tatcatcaaa tcgccagacg
    17761 catttcagtt catgggcgcg caggagcatt attccggatc gtatggcaac cgctattctg
    17821 aacgtagccg gtcgcacct
//
LOCUS       DAZKHF020000079        17366 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2201S31241
            SAMN43267800-rid25106413.denovo.079, whole genome shotgun sequence.
ACCESSION   DAZKHF020000079 DAZKHF020000000
VERSION     DAZKHF020000079.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267800
            Sequence Read Archive: SRR30307263
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 17366)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17366)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 17366)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350581.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350581.2
            Genome Coverage       :: 151x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:21:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,652
            CDSs (total)                      :: 5,557
            Genes (coding)                    :: 5,320
            CDSs (with protein)               :: 5,320
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 237
            CDSs (without protein)            :: 237
            Pseudo Genes (ambiguous residues) :: 0 of 237
            Pseudo Genes (frameshifted)       :: 102 of 237
            Pseudo Genes (incomplete)         :: 130 of 237
            Pseudo Genes (internal stop)      :: 52 of 237
            Pseudo Genes (multiple problems)  :: 41 of 237
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17366
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267800-rid25106413.denovo.079"
                     /serotype="O 26:H11"
                     /isolate="2201S31241"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-01"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            complement(155..1745)
                     /gene="lsrK"
                     /locus_tag="ACHOSC_RS10990"
                     /old_locus_tag="pgaptmp_002198"
                     /pseudo
     CDS             complement(155..1745)
                     /gene="lsrK"
                     /locus_tag="ACHOSC_RS10990"
                     /old_locus_tag="pgaptmp_002198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416028.3"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0071518 - autoinducer-2 kinase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0009372 - quorum sensing [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="autoinducer-2 kinase"
     gene            complement(1824..2777)
                     /gene="lsrR"
                     /locus_tag="ACHOSC_RS10995"
                     /old_locus_tag="pgaptmp_002199"
     CDS             complement(1824..2777)
                     /gene="lsrR"
                     /locus_tag="ACHOSC_RS10995"
                     /old_locus_tag="pgaptmp_002199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator LsrR"
                     /protein_id="HGU4548131.1"
                     /translation="MTINDSAISEQGMCEEEQVARIAWFYYHDGLTQSEISDRLGLTR
                     LKVSRLLEKGHQSGIIRVQINSRFEGCLEYETQLRRQFSLQHVRVIPGLADADVGGRL
                     GIGAAHMLMSLLQPQQMLAIGFGEATMNTLQRLSGFISSQQIRLVTLSGGVGSYMTGI
                     GQLNAACSVNIIPAPLRASSADIARTLKNENCVKDVLLAAQAADVAIVGIGAVSQQDD
                     ATIIRSGYISQGEQLMIGRKGAVGDILGYFFDAKGDVVTDIKIHNELIGLPLSALKTI
                     PVRVGVAGGENKAEAIAAAMKGGYINALVTDQDTAAAILRS"
     gene            3026..4561
                     /gene="lsrA"
                     /locus_tag="ACHOSC_RS11000"
                     /old_locus_tag="pgaptmp_002200"
     CDS             3026..4561
                     /gene="lsrA"
                     /locus_tag="ACHOSC_RS11000"
                     /old_locus_tag="pgaptmp_002200"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:1905887 - autoinducer AI-2 transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="autoinducer 2 ABC transporter ATP-binding
                     protein LsrA"
                     /protein_id="HGU4548132.1"
                     /translation="MQTSDTRALPLLCARSVYKQYSGVNVLKGIDFTLHQGEVHALLG
                     GNGAGKSTLMKIIAGITPADSGTLEIGGNNYARLTPVHAHQLGIYLVPQEPLLFPSLS
                     IKENILFGMAKKQLSMQKMKNLLAALGCQFDLHSLAGSLDVADRQMVEILRGLMRDSR
                     ILILDEPTASLTPAETERLFTRLQELLATGVGIVFISHKLPEIRQIADRISVMRDGTI
                     ALSGKTSELSTDDIIQAITPVVREKSLSASQKLWLELPGNRPQHAAGTPVLTLENLTG
                     EGFRNVSMTLNAGEILGLAGLVGAGRTELAETFYGLRTLRSGRIMLNGKEINKLSTGE
                     RLLRGLVYLPEDRQSSGLNLDASLAWNVCALTHNLRGFWAKTAKDNATLERYRRALNI
                     KFNQPEQAARTLSGGNQQKILIAKCLEASPQALIVDEPTRGVDVSARNDIYQLLRSIS
                     AQNVAVLLISSDLEEIELMTDRVYVMHQGEITHSALTGRDINVETIMRVAFGDSQRQE
                     ASC"
     gene            4555..5583
                     /gene="lsrC"
                     /locus_tag="ACHOSC_RS11005"
                     /old_locus_tag="pgaptmp_002201"
     CDS             4555..5583
                     /gene="lsrC"
                     /locus_tag="ACHOSC_RS11005"
                     /old_locus_tag="pgaptmp_002201"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="autoinducer 2 ABC transporter permease LsrC"
                     /protein_id="HGU4548133.1"
                     /translation="MLKFIQNNREITALLAVVLLFVLPGFLDRQYLSVQTLTMVYSSA
                     QILILLAMGATLVMLTRNIDVSVGSITGMCAVLLGMLLNAGYSLPVACVATLLLGLLA
                     GFFNGVLVAWLKIPAIVATLGTLGLYRGIMLLWTGGKWIEGLPAELKQLSAPLLFGVS
                     AIGWLTIILVAFMAWLLAKTAFGRSFYATGDNLQGARQLGVRTEAIRIVAFSLNGCMA
                     ALAGIVFASQIGFIPNQTGTGLEMKAIAACVLGGISLLGGSGAIIGAVLGAWFLTQID
                     SVLVLLRIPAWWNDFIAGLVLLAVLVFDGRLRCALERNLRRQKYARFMTPPPSVKPAS
                     SGKKREAA"
     gene            5583..6575
                     /gene="lsrD"
                     /locus_tag="ACHOSC_RS11010"
                     /old_locus_tag="pgaptmp_002202"
     CDS             5583..6575
                     /gene="lsrD"
                     /locus_tag="ACHOSC_RS11010"
                     /old_locus_tag="pgaptmp_002202"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autoinducer 2 ABC transporter permease LsrD"
                     /protein_id="HGU4548134.1"
                     /translation="MRIRYGWELALAALLVIEIVAFGAINPRMLDLNMLLFSASDFIC
                     IGIVALPLTMVIVSGGIDISFGSTIGLCAIALGVLFQSGVPMPLAILLTLLLGALCGL
                     INAGLIIYTKVNPLVITLGTLYLFAGSALLLSGMAGATGYEGIGGFPMAFTDFANLDV
                     LGLPVPLIIFLICLLVFWLWLHKTHAGRNVFLIGQSPRVALYSAIPVNRTLCALYAMT
                     GLASAVAAVLLVSYFGSARSDLGASFLMPAITAVVLGGANIYGGSGSIIGTAIAVLLV
                     GYLQQGLQMAGVPNQVSSALSGALLIVVVVGRSVSLHRQQIKEWLARRANNPLP"
     gene            6587..7609
                     /gene="lsrB"
                     /locus_tag="ACHOSC_RS11015"
                     /old_locus_tag="pgaptmp_002203"
     CDS             6587..7609
                     /gene="lsrB"
                     /locus_tag="ACHOSC_RS11015"
                     /old_locus_tag="pgaptmp_002203"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autoinducer 2 ABC transporter substrate-binding
                     protein LsrB"
                     /protein_id="HGU4548135.1"
                     /translation="MTLHRFKKIALLSALGIAAISMNVQAAERIAFIPKLVGVGFFTS
                     GGNGAQQAGKELGVDVTYDGPTEPSVSGQVQLINNFVNQGYNAIIVSAVSPDGLCPAL
                     KRAMQRGVRVLTWDSDTKPECRSYYINQGTPAQLGGMLVDMAARQVNKDKAKVAFFYS
                     SPTVTDQNQWVKEAKAKIAKEHPGWEIVTTQFGYNDATKSLQTAEGILKAYSDLDAII
                     APDANALPAAAQAAENLKNDKVAIVGFSTPNVMRPYVERGTVKEFGLWDVVQQGKISV
                     YVADALLKKGSMKTGDKLDIQGVGQVEVSPNSVQGYDYEADGNGIVLLPERVIFNKEN
                     IGKYDF"
     gene            7636..8472
                     /gene="lsrF"
                     /locus_tag="ACHOSC_RS11020"
                     /old_locus_tag="pgaptmp_002204"
                     /pseudo
     CDS             7636..8472
                     /gene="lsrF"
                     /locus_tag="ACHOSC_RS11020"
                     /old_locus_tag="pgaptmp_002204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_462959.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxy-5-phosphonooxypentane-2,4-dione
                     thiolase"
     gene            8471..8965
                     /locus_tag="ACHOSC_RS11025"
                     /old_locus_tag="pgaptmp_002205"
                     /pseudo
     CDS             8471..8965
                     /locus_tag="ACHOSC_RS11025"
                     /old_locus_tag="pgaptmp_002205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707457.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
     gene            complement(8969..9883)
                     /gene="yneE"
                     /locus_tag="ACHOSC_RS11030"
                     /old_locus_tag="pgaptmp_002206"
     CDS             complement(8969..9883)
                     /gene="yneE"
                     /locus_tag="ACHOSC_RS11030"
                     /old_locus_tag="pgaptmp_002206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416037.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bestrophin family protein"
                     /protein_id="HGU4548136.1"
                     /translation="MIVRPQQHWLRRIFVWHGSVLSKISSRLLLNFLFSIAVIFMLPW
                     YTHLGIKFTLAPFSILGVAIAIFLGFRNNAGYARYVEARKLWGQLMIASRSLLREVKT
                     TLPDSASVREFARLQIAFAHCLRMTLRKQPQAEVLAHYLKTEDLQPVLASNSPANRIL
                     LIMGEWLAVQRRNGQLSDIQFISLNDRLNDISAVLAGCERIAYTPIPFAYTLILHRTV
                     YLFCIMLPFALVVDLHYMTPFISVLISYTFISLDCLAEELEDPFGTENNDLPLDAICN
                     AIEIDLLQMNDEAEIPAKILPDRHYQLT"
     gene            complement(10090..11541)
                     /gene="uxaB"
                     /locus_tag="ACHOSC_RS11035"
                     /old_locus_tag="pgaptmp_002207"
     CDS             complement(10090..11541)
                     /gene="uxaB"
                     /locus_tag="ACHOSC_RS11035"
                     /old_locus_tag="pgaptmp_002207"
                     /EC_number="1.1.1.58"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagaturonate reductase"
                     /protein_id="HGU4548137.1"
                     /translation="MKTLNRRDFPGAQYPERIIQFGEGNFLRAFVDWQIDLLNEHTDL
                     NSGVVVVRPIETSFPPSLSTQDGLYTTIIRGLNEKGEAVSDARLIRSVNREISVYSEY
                     DEFLKLAHNPEMRFVFSNTTEAGISYHAGDKFDDAPAVSYPAKLTRLLFERFSHFNGA
                     LDKGWIIIPCELIDYNGDALRELVLRYAQEWALPEAFIQWLDQANSFCSTLVDRIVTG
                     YPRDEVAKLEEELGYHDGFLDTAEHFYLFVIQGPKSLATELRLDKYPLNVLIVDDIKP
                     YKERKVAILNGAHTALVPVAFQAGLDTVGEAMNDAEICAFVEKAIYEEIIPVLDLPRD
                     ELESFASAVTGRFRNPYIKHQLLSIALNGMTKFRTRILPQLLARQKAKGTLPARLTFA
                     LAALIAFYRGERNGETYPVQDDAHWLERYQQLWSQHRDRVIGTQELVAIVLAEKDHWE
                     QDLTQVPGLVEQVANDLDAILEKGMREAVRPLC"
     gene            complement(11768..13186)
                     /locus_tag="ACHOSC_RS11040"
                     /old_locus_tag="pgaptmp_002208"
     CDS             complement(11768..13186)
                     /locus_tag="ACHOSC_RS11040"
                     /old_locus_tag="pgaptmp_002208"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310156.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HGU4548138.1"
                     /translation="MHVHPISTFRLFQEGHLLRNSIAIFALTTLFYFIGAELRLVHEL
                     SLFWPLNGVMAGVFARYVWLNRLHYYAISYVAMLVYDAITTEWGLVSLVINFSNMMFI
                     VTVALLVVRDKRLGKNKYEPVSALRLFNYCLIAALLCAIVGAIGSVSIDSLDFWPLLA
                     DWFSEQFSTGVLIVPCMLTLAIPGVLPRFKAEQMMPAIALIVSVIASVVIGGAGSLAF
                     PLPALIWCAVRYTPQVTCLLTFVTGAVEIVLVANSVIDISVGSPFSIPEMFSARLGIA
                     TMAICPIMVSFSVAAINSLMKQVALRADFDFLTQVYSRSGLYEALKSPSLKQTQHLTV
                     MLLDIDYFKSINDNYGHECGDKVLSVFARHIQKIVGDKGLVARMGGEEFAVAVPSVNP
                     VDGLLMAEKIRKGVELQPFTWQQKTLYLTVSIGVGSGRASYRTLTDDFNKLMVEADTC
                     LYRSKKDGRNRTSTMRYGEEVV"
     gene            complement(13325..13684)
                     /gene="yneG"
                     /locus_tag="ACHOSC_RS11045"
                     /old_locus_tag="pgaptmp_002209"
     CDS             complement(13325..13684)
                     /gene="yneG"
                     /locus_tag="ACHOSC_RS11045"
                     /old_locus_tag="pgaptmp_002209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4186 domain-containing protein"
                     /protein_id="HGU4548139.1"
                     /translation="MQSLDPLFARLSRSKFRSRFRLGMKERQYCLEKGAPVIEQHAAD
                     FVAKRLAPALPANDGKQTPMRGHPVFIAQHATATCCRGCLAKWHNIPQGVSLSEEQQR
                     YIVAVIYHWLVVQMNQP"
     gene            complement(13684..14610)
                     /gene="glsB"
                     /locus_tag="ACHOSC_RS11050"
                     /old_locus_tag="pgaptmp_002210"
     CDS             complement(13684..14610)
                     /gene="glsB"
                     /locus_tag="ACHOSC_RS11050"
                     /old_locus_tag="pgaptmp_002210"
                     /EC_number="3.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004359 - glutaminase activity [Evidence
                     IEA];
                     GO_process: GO:0006541 - glutamine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutaminase B"
                     /protein_id="HGU4548140.1"
                     /translation="MAVAMDNAILENILRQVRPLIGQGKVADYIPALATVDGSRLGIA
                     ICTVDGQLFQAGDAQERFSIQSISKVLSLVVAMRHYSEEEIWQRVGKDPSGSPFNSLV
                     QLEMEQGIPRNPFINAGALVVCDMLQGRLSAPRQRMLEVVRGLSGVSDISYDTVVARS
                     EFEHSARNAAIAWLMKSFGNFHHDVTTVLQNYFHYCALKMSCVELARTFVFLANQGKA
                     IHIDEPVVTPMQARQINALMATSGMYQNAGEFAWRVGLPAKSGVGGGIVAIVPHEMAI
                     AVWSPELDDAGNSLAGIAVLEQLTKQLGRSVY"
     gene            complement(14674..16062)
                     /gene="sad"
                     /locus_tag="ACHOSC_RS11055"
                     /old_locus_tag="pgaptmp_002211"
     CDS             complement(14674..16062)
                     /gene="sad"
                     /locus_tag="ACHOSC_RS11055"
                     /old_locus_tag="pgaptmp_002211"
                     /EC_number="1.2.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416042.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004030 - aldehyde dehydrogenase [NAD(P)+]
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinate-semialdehyde dehydrogenase"
                     /protein_id="HGU4548141.1"
                     /translation="MTITPATHAISINPATGEQLSVLPWAGANDIENALQLAAAGFRD
                     WRETNIDYRAEKLRGIGKALRARSEEMAQMITREMGKPINQARAEVAKSANLCDWYAE
                     HGPAMLKAEPTLVENQQAVIEYRPLGTILAIMPWNFPLWQVMRGAVPIILAGNGYLLK
                     HAPNVMGCAQLIAQVFKDAGIPQGVYGWLNADNDGVSQMIKDSRIAAVTVTGSVRAGA
                     AIGAQAGAALKKCVLELGGSDPFIVLNDADLELAVKAAVAGRYQNTGQVCAAAKRFII
                     EEGIASAFTERFVAAAAALKMGDPRDEENALGPMARFDLRDELHHQVEKTLAQGARLL
                     LGGEKMAGAGNYYPPTVLANVTPEMTAFREEMFGPVAAITVAKDAEHALELANDSEFG
                     LSATIFTTDETQARQMAARLECGGVFINGYCASDARVAFGGVKKSGFGRELSHFGLHE
                     FCNIQTVWKDRI"
     gene            16163..17044
                     /gene="yneJ"
                     /locus_tag="ACHOSC_RS11060"
                     /old_locus_tag="pgaptmp_002212"
     CDS             16163..17044
                     /gene="yneJ"
                     /locus_tag="ACHOSC_RS11060"
                     /old_locus_tag="pgaptmp_002212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR substrate-binding domain-containing
                     protein"
                     /protein_id="HGU4548142.1"
                     /translation="MDLTQLEMFNAVAEAGSITQAAAKVHRVPSNLTTRLRQLETELG
                     VDLFIRENQRLRLSPAGHNFLRYSQQILALVDEARSVVAGDEPQGLFSLGSLESTAAV
                     RIPATLAEFNHRYPKIQFSLSTGPSGTMLEGVLEGKLNAAFIDGPINHTAIDGIPVYR
                     EELMIVTPQGHAPVIRASQVNGSNIYAFRANCSYRRHFESWFHADGAAPGTIHEMESY
                     HGMLACVVAGAGIALIPRSMLESMPGHHQVEAWPLAEQWRWLTTWLVWRRGAKTRPLE
                     AFIQLLDVPDSAKQGYQ"
     gene            17122..>17366
                     /gene="yneK"
                     /locus_tag="ACHOSC_RS11065"
                     /old_locus_tag="pgaptmp_002213"
     CDS             17122..>17366
                     /gene="yneK"
                     /locus_tag="ACHOSC_RS11065"
                     /old_locus_tag="pgaptmp_002213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative protein YneK"
                     /protein_id="HGU4548143.1"
                     /translation="MVTPVSISNYISLPDDFPVRNIAPQVKEVLKDFIDALSTIICDE
                     EWRTSLNINSATKKIFNNLDNLSYIQRTSFRGNDTLY"
BASE COUNT         4359 a         4455 c         4314 g         4238 t
ORIGIN      
        1 aaaaatagca attttccctt gaatatagta ctggtgatgg aacgatgaat ctgcaagctg
       61 gcatttaaca agccagctct aaaagaaggg aaataagaaa aactatactc aaaaactaac
      121 agccacggtc atcatgatgt ggccgtcaat gaaactataa cccaggcgct ttccataacg
      181 acgtcgtcag tccatgatca accagcccca gctgatcctg ataaactgcc tgccacttat
      241 cgcgtgaatc ctgataaagt tcatgctttt ccgggtctgg tgtgtgcgtc cgttcccagc
      301 gaaccaggcg ttctccggtt tctgccattg atgaaaaaat tccggcaccg acgccagctg
      361 caatggcaca tcctaatgcg gtggcttctt tgactaccgg aatattgacg ggtaatcccg
      421 agacatcagc gagaatttga ctccataatt tcccttttga acctccgcca gcaaaaacca
      481 gcgaggtggg atgaatattc gagaaatcag caatttgctg caagttacac gctgatacaa
      541 tcgccgcatt ttcttccagc gcacggaaca atgtcgcttt gttacattta tccgggtcaa
      601 tggacaagtt aataaaggaa ggcgcagcgt gataccaggt tttaaagcgc attctgtcgg
      661 agaagatcgg cattacgccc cacgacccag gcggcacccg actggccatc tcttccagca
      721 gcgtataggt gtcgatgcct aaacgttcgg caatcagttt ttcttcggca cagaaagcat
      781 cgcggaacca gcgcatggtg agtccggtaa aaaagcttat agattcagct tgtaccatgc
      841 caggaataac atgaggatta acgcgcacgt tcatttctgg gtctgtcacc ggcgcggcta
      901 aatttacaac ttgctgccag aatgtgccgc caagaaccgc ggtttgtgcc ggacgcacaa
      961 cgcctaaccc aaggcaacca agctgcacgt cgcctcctcc aacgaccacc ggagtgcccg
     1021 ccttcagacc gcagagttcc gccgcttgtg aacttaccac gcccagcaat gtgccggttt
     1081 ctttgacagg agaaagaata tcggcacgca ggccagccat atccagcaat gcaggtttcc
     1141 agtcacgggt ggttagatca aagtcccgtg gtgccagcgt tagagggatc caccgccagt
     1201 tcgccgctga gcatataggc cagccagtcg ctgatcatgg tgatggttga tgcctgacgg
     1261 taaatatcgg aacgatggtg cgccagccaa agtaatctgg ggatggcact taaagccagt
     1321 gtttgtccgg tcgcgcgata aacttcgttt tcaaaggtat tgttgtgcag ttctttaagt
     1381 tcgctaactt cgcgcgctgc tctggcatcc acattggcgc aggcccagat cggtgttcct
     1441 tcattgttat ataaaacaat gccttcacgc atagaacatg ccgaaacggc agcgatatac
     1501 tccgggtcta tgccggcgtt gtgcagcgcc tggcgcatac actcacacgc cagttgccag
     1561 tttttgttga gatcaaattc catagaacca ggaacgtccg gtactgccag atgccgccac
     1621 tccgcctgtc ccactgctat ttgattgcct ttcaggtcga aaatcacagc ccgaatactt
     1681 ccggtgcctg catccagcgc caacaggtaa tactttgatt ctgaaggggt aaagagtcga
     1741 gccattagat tatcctcggc ttatgcgttc tgtctgttcc tctatacgtt ctccatcatt
     1801 cccggtaata aggtcatgca aatttaacta cgtaaaatcg ccgctgctgt atcctgatcg
     1861 gtaaccagtg cgttgatata accgcctttc attgcagcgg caattgcttc ggctttattt
     1921 tctccccctg ccacgccaac ccggacgggt atggtcttca gcgcgcttaa aggtaagcca
     1981 atcagttcgt tatgtatttt aatatccgtg acaacgtcac cttttgcatc aaaaaagtag
     2041 cctaaaatgt cgccaaccgc cccttttcga ccaatcatta actgttcgcc ctggctgata
     2101 taaccggagc gaatgatcgt cgcgtcgtcc tgttgactga cggccccaat gccgacaatc
     2161 gccacatccg ctgcttgcgc ggctaacaga acatctttga cgcaattttc attttttagc
     2221 gtacgggcaa tgtcagcgga ggatgcccgc aacggagccg gaataatatt cacactgcac
     2281 gccgcgttaa gctgcccgat acccgtcata taagaaccga cgccaccgga gagcgtgacc
     2341 aggcgaattt gctgtgacga aataaaacca cttaagcgtt gcagcgtatt catggttgcc
     2401 tcgccaaaac caatcgccag catctgttgt ggttgaagta aactcatcaa catatgcgcc
     2461 gcgcctatcc ctagtcgccc gccgacatca gcatccgcaa gcccagggat cacccggaca
     2521 tgttgcagcg aaaactgacg acgtaattga gtttcatatt ccagacagcc ttcaaagcga
     2581 gaattaatct gtacgcgaat aatgccggac tgatgccctt tctccagcaa gcgcgacact
     2641 ttcaaacggg tcaggccgag acgatcgctg atctcgctct gggtcagccc gtcgtgatag
     2701 taaaaccacg cgatccgcgc gacctgttct tcttcacaca ttccctgttc tgaaattgcc
     2761 gaatcgttga ttgtcataat tcattcttca ctttgaacat atttaaatct ttaatgcaat
     2821 tgttcagttc ttgcgcattt atatctgtga tggcagccac agtttgactc tacgagcatg
     2881 aacaaacgca accgtgaaaa tcaaaatagc ataaattgtg atctattcgt cggaaatatg
     2941 tgcaatgtcc acctaaggtt atgaacaaat taaaagcaga aatacatttg ttcaaaactc
     3001 acctgcaaaa ctgaacgggg gaaatatgca aacgagtgat acccgcgcgt taccgctact
     3061 ttgcgcccgc tcggtttata aacagtattc aggggtcaat gtcctgaaag gcatcgattt
     3121 tacgttgcat cagggggagg tccacgccct gctcggcggc aatggtgccg gtaaatcgac
     3181 gttaatgaag attattgccg gtattacccc tgctgatagc ggtacgctgg agattggggg
     3241 caacaactac gccagattaa cgccagttca tgctcatcag ctgggcattt atctcgttcc
     3301 ccaggaaccg ctgcttttcc caagcctgtc gataaaagaa aacatcctgt ttgggatggc
     3361 aaaaaaacag ctctccatgc aaaaaatgaa gaacttgctg gcggcgctgg gctgccagtt
     3421 tgatctgcat agtctggcag gatcgctgga tgtcgccgat cgccaaatgg tggaaatcct
     3481 ccgcgggcta atgcgcgact cgcggattct gatcctcgat gaacctaccg cctcgcttac
     3541 ccctgcggaa accgaacgct tgtttactcg cttgcaagag ctgcttgcta ctggcgtggg
     3601 tattgttttt atctcgcata agctgccgga aattcgccag attgccgatc gaattagcgt
     3661 gatgcgcgac ggaaccatcg ccttaagcgg caaaaccagc gaactgtcta ccgacgacat
     3721 tattcaggcc atcacgccag tggtacggga aaaatcgctc tctgccagcc aaaaattatg
     3781 gctggagtta cctggtaacc gcccacaaca tgctgccgga acgccggtgc tgacactgga
     3841 aaatctgacc ggcgaaggtt tcaggaatgt cagcatgacg ctcaatgccg gagaaattct
     3901 gggcctggct gggctggtgg gggccggacg cacagaactg gccgagacgt tctatggtct
     3961 gcgtactttg cgtagcggac gcattatgct gaatggtaaa gagatcaata aattatccac
     4021 tggagaacgt ttactgcgcg gtctggttta tctgccggaa gatcgccagt catccggact
     4081 gaatctcgat gcttcgctgg cctggaacgt ctgcgccctt actcataacc ttcgtggatt
     4141 ctgggcgaaa accgcgaaag ataatgccac cctggaacgt tatcgtcggg cgctgaatat
     4201 taaatttaac caaccggaac aagctgcacg gacattatcc ggtggcaacc agcaaaaaat
     4261 cctcattgcc aaatgcctgg aagcttcgcc gcaagcattg attgtcgatg agccgacgcg
     4321 cggcgtggat gtctcggccc gtaatgatat ctaccagctg ttgcgcagca tctccgcaca
     4381 gaatgtggct gtgctgctta tctcctctga tctggaagag atcgaactga tgacagatcg
     4441 tgtgtatgtg atgcatcagg gcgaaattac ccactctgca ctgaccgggc gcgatattaa
     4501 tgtcgagact attatgcgcg ttgccttcgg cgatagtcag cgtcaggagg catcatgctg
     4561 aagtttattc agaacaaccg tgaaatcacg gcattgctgg cggtggtgct gctgtttgta
     4621 ttacccggtt ttctcgaccg tcagtattta agtgtgcaaa cgctgaccat ggtttatagc
     4681 agcgcgcaaa tcctgatcct gctggcaatg ggcgcgacac tggtaatgct tacgcgcaat
     4741 attgatgttt cagtgggttc gattaccgga atgtgcgcgg tgctgttggg gatgttactg
     4801 aacgcaggat attcactacc tgttgcctgt gtcgcgactt tactgcttgg tttgctcgcg
     4861 ggatttttca acggtgtcct ggtcgcgtgg ctaaagatcc ctgccattgt tgccaccctt
     4921 ggcacgttag ggttgtacag aggcatcatg ttgctgtgga ctggcggtaa atggattgaa
     4981 gggttacccg ccgaactgaa acagctctcc gcaccgctgc tgtttggcgt ttccgcgatt
     5041 ggttggttga cgataattct ggtggcattt atggcctggc tgctggcaaa gacggcgttt
     5101 ggacgcagtt tttatgccac gggcgataat ttacagggcg ctcgtcaact gggtgttcgt
     5161 acggaagcca ttcgcattgt ggcattttcg ttgaacggct gcatggcggc actggcggga
     5221 attgtgtttg cttcgcagat tggttttatc cccaaccaga ccggtaccgg gctggagatg
     5281 aaagcaattg cagcctgcgt gctgggcggc attagtttgc tcggcggttc cggtgcgatc
     5341 atcggtgcgg tactcggcgc atggttcctg acgcagatcg atagcgtact ggtgctgttg
     5401 cgcattccgg catggtggaa cgattttatc gcgggcctcg tgctgctggc ggtgctggtg
     5461 tttgatggac gcctgcgttg tgcgctggaa cgtaatctac ggcggcaaaa atatgcccgc
     5521 tttatgacgc caccgccatc cgttaaaccc gcttcgtcag gtaaaaaacg ggaggccgca
     5581 taatgcgtat tcgctacggt tgggaactgg ctcttgccgc actgctcgtt attgagattg
     5641 tcgcatttgg tgcaattaac ccgcgaatgt tagatctcaa tatgttgctg ttcagcgcca
     5701 gtgactttat ctgcattggc attgtcgccc taccgctgac gatggtgatt gtcagtggcg
     5761 ggatcgatat ttcgtttggt tcgaccatcg gcctctgcgc cattgcattg ggcgtactgt
     5821 ttcaaagtgg tgtgccgatg ccgctggcga tactcctgac cttactgctc ggcgcattgt
     5881 gcgggctgat caacgccgga ttaattatct ataccaaagt taacccgctg gtgattacgc
     5941 ttggcacgct gtatctgttt gccggaagcg ctctgctgct ttccggtatg gccggagcga
     6001 cggggtacga aggtattggt ggattcccga tggcgtttac agatttcgct aacctggatg
     6061 tgctgggact ccccgttccg ctgattatct tcctgatatg tctcctcgtt ttctggctct
     6121 ggctgcataa aacccatgcc ggacgtaatg tgtttttgat tgggcaaagc ccgcgcgtgg
     6181 cgctttatag cgcgattcca gttaaccgca ccttatgtgc gctctatgcc atgacggggc
     6241 tggcgtctgc ggtcgccgct gtgctgctgg tatcgtattt tggttcagca cgttccgatc
     6301 tcggtgcgtc gtttctgatg cccgccatca ccgccgtggt gcttggcggg gccaatattt
     6361 atggtggttc cggttccatt atcggcaccg ccattgcggt tttattagtg ggatatttgc
     6421 agcaaggttt gcaaatggca ggagtgccaa atcaggtgtc cagcgccctt tccggtgcgc
     6481 tacttatcgt cgttgtcgta ggtcgttccg ttagcctgca tcgccagcaa attaaagagt
     6541 ggctggcgcg tcgggccaat aacccattgc cataaaggat atcttcatga cacttcatcg
     6601 ctttaagaaa atcgccttac ttagcgctct tggcattgcc gcaatctcta tgaatgtgca
     6661 ggccgcagag cgtattgcat ttattcccaa actggttggc gtgggatttt ttaccagcgg
     6721 tggcaacggc gcacaacaag cgggtaaaga gctgggcgtt gatgtgacct acgacgggcc
     6781 gacagaaccc agtgtttctg gtcaggtaca gttgattaat aacttcgtca atcaaggtta
     6841 taacgccatt atcgtttctg cggtttcgcc tgatggcttg tgtccggcac tgaaacgcgc
     6901 catgcaacgt ggtgtgagag tgctgacctg ggactctgat actaaaccgg agtgccgctc
     6961 ttactacatt aatcagggaa cgcccgccca gttgggaggt atgttggtgg atatggcggc
     7021 gcgtcaggtg aataaagaca aagccaaagt cgcgtttttc tactcaagcc ccaccgttac
     7081 ggaccaaaac cagtgggtga aagaagcgaa agcgaaaatc gccaaagagc atcctggctg
     7141 ggaaattgtc actacgcagt ttggctataa cgatgccact aaatcattac aaaccgcaga
     7201 aggaatatta aaagcgtata gcgatctcga cgccattatc gcccccgacg ccaacgccct
     7261 gcccgctgcc gcacaagccg cagaaaacct gaaaaatgac aaagttgcga ttgtcggatt
     7321 cagtacgcca aacgtgatgc gtccatatgt ggaacgcggc acggtgaaag aatttggcct
     7381 gtgggatgtg gttcagcaag gcaaaatttc agtgtatgtc gcggatgcat tattgaaaaa
     7441 aggatcaatg aaaacgggcg acaagctgga tatccagggc gtaggtcagg tggaagtctc
     7501 gccaaacagc gttcagggct atgactacga agcggatggt aatggcatcg tactgttacc
     7561 ggagcgcgtg atattcaaca aagagaatat cggcaaatac gatttctgat gtgcattact
     7621 taaccggagt aagttatggc agatttagac gatattaaag atggtaaaga ttttcgtacc
     7681 gatcaatcgc aacaaaatat cccttttatc ctgaaaggtt gcggtgcgct ggattgggga
     7741 atgcagtcac gcttatcgcg gatatttaat ccgaaaacgg gtaaaaccgt gatgctggcc
     7801 tttgaccatg gttattttca ggggccgact accggacttg aacgcattga tataaatatc
     7861 gccccgctgt ttgaacatgc cgatgtatta atgtgtacgc gcggcatttt gcgcagcgta
     7921 gttcccccag cgaccaataa gccggtggta ctgcgggcgt caggagcgaa ctctattctg
     7981 gcggaattaa gtaatgaagc cgtggcgtta tcgatggatg atgccgtgcg cctgaacagt
     8041 tgcgcggtgg cggcgcaggt ttatatcggc agcgaatatg aacatcagtc gatcaaaaat
     8101 attattcagc tggttgatgc cggaatgaaa gtgggaatgc cgaccatggc cgtgactggc
     8161 gtgggcaaag atatggtgcg cgatcagcgt tatttctcgc tcgcgactcg aatcgccgct
     8221 gaaatggggg cgcaaattat caaaacctat tatgtcgaaa aaggttttga acggattgtt
     8281 gccggatgtc cggtacccat tgttattgct ggcggtaaaa aattaccgga gcgcgaggcg
     8341 ctggaaatgt gctggcaggc tatcgatcag ggcgcttctg gtgtggatat ggggcgtaat
     8401 attttccagt ctgaccatcc ggtggcgatg atgaaagccg tacaggcggt ggttcaccat
     8461 aacgaaacgg caaccggaac aggcactcga tctgattttt gctaatgcct cactgcaatg
     8521 gctgcccgac cactacgaat tgtttcctca tctggtttct ttacttaatc cgcagggcgt
     8581 gctggcagta cagatgccag ataactggct ggagccgacc catgtgctca tgcgcgaagt
     8641 tgcctgggaa caaaactacc cggatcgcgg gcgtgagccg ttggctggcg ttcatgctta
     8701 ctacgatatt ttgagcgaag ccggatgtga ggtcgatatc tggcgaacca cttactatca
     8761 ccagatgccg tcgcaccagg cgattatcga ttgggtgacc gccactggat tacgtccgtg
     8821 gttacaggat ctgaccgaga gcgaacagca gctttttctt acgcgctacc atcagatgct
     8881 ggaagagcag tatccactgc aagagaacgg acagatactg ctggcatttc cgcgtctgtt
     8941 tattgttgcc cgccgtacgg agtaattatc acgtcagctg gtaatgacga tcgggaagaa
     9001 tttttgctgg aatttctgct tcatcgttca tctgtaacag gtcaatttca atagcgttgc
     9061 agatggcatc cagcggtaaa tcattgtttt cagtaccgaa cggatcttcc agttcttccg
     9121 ccagacaatc cagcgaaata aaagtgtagg aaatcagcac agagataaaa ggcgtcatgt
     9181 aatgcaggtc cacgaccagc gcgaacggca gcatgataca aaacagataa acggtacgat
     9241 gcaaaatcag ggtgtaggca aagggaattg gcgtgtaggc aatgcgctca cagcctgcca
     9301 gaaccgctga aatatcatta agccgatcgt tgaggctaat aaactggata tctgaaagct
     9361 gcccattgcg gcgctgaacc gccaaccact cccccattat taacaagata cggttagctg
     9421 gagagttcga agccagtaca ggctgaagat cttcagtctt gagataatga gccagcactt
     9481 ccgcctgtgg ctgtttgcgt aatgtcatgc gtaaacagtg ggcgaaggcg atttgcaacc
     9541 gggcaaactc ccttacactt gccgaatccg gcaatgtcgt ttttacctcg cgcagtaacg
     9601 accgtgaggc aatcatcaac tgcccccaaa gttttcgcgc ttcaacgtaa cgggcgtacc
     9661 cggcattatt acgaaaacca agaaaaatgg cgatggcgac accgagaatg ctgaacggtg
     9721 cgagggtgaa tttgatgccc agatgcgtgt accagggcag catgaaaata acagcgatag
     9781 aaaagagaaa attgagtagt aagcgcgatg atatcttgga taatactgag ccgtgccaga
     9841 caaaaatacg gcgcagccag tgttgttgtg gacgaacaat catggttatc ttcaggcgtg
     9901 aaaaagtcgc cctattaaac gtgattacga tcacattctc aagacgctac ttacaaatta
     9961 cctacaagct tacaacatta acaggcgctg catgtggcat ctgttaatga taaaggcaca
    10021 taggatgttg taactaatat agtgaaataa gaaacccggt cgaaaccggg ttcagaagta
    10081 acggtgttat tagcacaacg gacgtacagc ttcgcgcatc cctttttcaa gaatcgcatc
    10141 caggtcatta gcaacctgct cgaccagacc aggcacttgc gtcaggtcct gctcccagtg
    10201 gtctttttct gccaacacaa tcgctaccag ttcctgggta ccgataacac ggtcacgatg
    10261 ctggctccag agttgctgat aacgttccag ccagtgtgca tcgtcctgta ccggatatgt
    10321 ttctccgtta cgctcaccac gatagaaggc aatcaatgcc gctaatgcga aagtaaggcg
    10381 cgccggaagt gtgccttttg ccttctgccg tgccagcagc tgcggcagga tgcgagtgcg
    10441 gaacttggtc ataccgttga gcgcgataga cagcagctga tgcttaatgt acgggttacg
    10501 gaaacgcccg gtgactgcac tggcgaaaga ttccagttca tcacgaggca aatccagtac
    10561 cggaataatt tcttcgtaga tagctttttc aacgaatgcg caaatttcag catcgttcat
    10621 cgcttcacct acggtatcca gccctgcctg gaacgccacc ggcaccagcg cggtgtgcgc
    10681 accgttgagg attgccactt tgcgttcttt gtacggttta atatcgtcaa caatcagcac
    10741 gttgagcgga tatttgtcca gacgcagttc agtcgctaag gatttcggtc cctgaatcac
    10801 aaacaggtaa aagtgttcag cggtgtcgag aaaaccatcg tgataaccca gctcttcttc
    10861 cagtttagcc acttcatcgc gcggataacc ggtaacgata cggtccacca gcgtagaaca
    10921 gaagctgtta gcctgatcca gccattgaat aaatgcttct ggcagtgccc actcttgcgc
    10981 atagcgcagc accagttcac gcaacgcgtc gccattgtag tcgatcaact cacacgggat
    11041 gatgatccaa cctttatcca gcgcaccgtt gaaatggcta aaacgttcga acagcagacg
    11101 ggtcagtttt gccggataac ttaccgctgg cgcgtcatcg aatttatcgc ccgcatggta
    11161 gctgataccc gcttcggtgg tattagagaa aacaaagcgc atttccgggt tgtgagccag
    11221 tttcaggaat tcatcgtatt cactgtagac gctgatttca cgattaaccg agcgaatcag
    11281 acgcgcgtcg ctgaccgctt cccccttctc attcaggcca cggataatgg tggtatacag
    11341 accatcctgc gtgctcagtg acggtgggaa tgaagtttca atcggacgaa caacgaccac
    11401 accagaattc agatcggtgt gctcattcag gagatcgatt tgccagtcaa caaaggcgcg
    11461 caggaagtta ccttcaccaa actgaatgat acgttctgga tactgtgcac cgggaaaatc
    11521 gcgacgattt agtgttttca caatgggttt ccttctgatt agtcatacaa cctgtttgaa
    11581 ttggtacgac aggttagcaa actttaatac gccgaacccc tgttttgatc aactcctgat
    11641 gattaatgag cagttttatg agaaaagtgt ggcgcggatc atggtttaat cgaggaaata
    11701 cgccttttcc tggatcataa agtggtagaa cacattgcat tcaaatcgcg cggaatgaat
    11761 aaagatgtca gacaacttcc tcaccgtaac gcatagtgct ggtacggttg cgcccatctt
    11821 tcttcgagcg atacagacac gtatcggctt cgaccatcaa tttattaaaa tcatcggtca
    11881 gcgttcggta cgatgcgcga ccactaccga cgccaatact taccgtgaga taaagcgttt
    11941 tttgttgcca ggtgaatggt tgcagttcaa cgcctttacg gattttttcc gccattagca
    12001 gaccatcgac aggattcacc gacggcactg caacagcaaa ttcttcgccg cccatgcgcg
    12061 ccaccagccc cttatcaccg acaatcttct gaatatgccg ggcaaacacg cttaacactt
    12121 tatcgccaca ttcatgtcca tagttatcgt taatgctttt gaaatagtcg atatcaagca
    12181 gcatgaccgt cagatgttgt gtctgtttca gcgatggact tttcagcgcc tcataaagac
    12241 cggaccgtga gtaaacctga gtcagaaaat caaagtcggc tcgcagcgca acttgcttca
    12301 ttagcgaatt gatcgctgcc acgctaaaag aaaccataat tgggcatatc gccatcgtgg
    12361 caataccgag acgtgcggag aacatttccg gaatggagaa cggcgaaccg accgagatat
    12421 caatcaccga atttgccacc agtacgattt ccaccgcacc ggtgacaaag gtcaacagac
    12481 atgttacctg cggcgtatag cgcactgcac accagattaa tgcaggcagc gggaacgcca
    12541 gactccccgc tccgccaatg actaccgagg caataaccga cacaataagc gcgatggcgg
    12601 gcatcatctg ttctgcttta aagcgcggca gcactccagg aatagccaac gtcagcatac
    12661 aaggcacgat caacacgccc gttgagaatt gctcactgaa ccagtccgca agcaaaggcc
    12721 agaaatccag actatcaata ctgaccgaac caatcgcccc gacaatagcg cataataatg
    12781 cggcaatcag acagtaatta aatagccgta aggcacttac tggctcatac ttatttttcc
    12841 ccagacgctt atcccgcacg accagtaagg cgacggtaac aataaacatc atattggaga
    12901 aattgataac cagtgaaacc agtccccatt cggtggttat ggcatcataa accagcatcg
    12961 ccacataact gatcgcatag taatgcagtc gattaagcca gacatagcgg gcaaacaccc
    13021 ccgccattac gccattcagc ggccagaaaa gagaaagttc gtgaaccagc cgtaactctg
    13081 caccaataaa atagaacagc gtggttagcg caaaaatagc gatgctatta cgtagcagat
    13141 gaccttcctg gaacaaacgg aaagttgaaa tggggtgtac atgcattaat tatctgtcca
    13201 tacaattgcg ggtggcaatt atctcaacgt aataagtaaa ttctcataaa ggatagcatg
    13261 ttcgctaaca gcggcggcaa ataaaggtac cctgggaatg ctattttatc gttttccgca
    13321 attctcatgg ctgattcatc tgtacaacca gccagtgata gattaccgcc acgatataac
    13381 gttgttgttc ttcgcttaac gaaacaccct gcggaatgtt atgccactta gccagacaac
    13441 cgcgacagca ggtcgcggtg gcgtgctgag cgataaacac cggatgcccg cgcatggggg
    13501 tttgcttacc gtcattagcc ggtaatgccg gagcaagacg tttagcgaca aaatccgccg
    13561 catgttgttc gatgactggc gcgcctttct ccaggcaata ctgacgctct ttcatgccca
    13621 gacgaaagcg agagcgaaat tttgaacggg ataaacgcgc gaagagtgga tcgagagact
    13681 gcattaataa accgaacgcc ctaactgttt cgtcaattgt tcaaggacgg caatacccgc
    13741 aagcgagtta cctgcatcat ccagttccgg actccagaca gcgatggcca tttcatgcgg
    13801 aacaatcgcc acaataccgc caccaacgcc agatttcgcc ggtagcccca cccgccaggc
    13861 aaactccccc gcgttctggt acataccact ggtcgccatc agcgcgttaa tttgccgcgc
    13921 ctgcattggc gtcaccacag gttcatcaat atgaatagct ttcccctgat tagccagaaa
    13981 gacaaacgtc ctggccagct ctacacagct cattttcaga gcgcagtaat gaaagtagtt
    14041 ttgcagaacg gttgtcacat catgatggaa attgccaaac gacttcatca gccaggcgat
    14101 agccgcattt cgcgcggaat gttcaaattc ggaacgcgct accaccgtat cgtatgaaat
    14161 atcagacaca ccgcttaagc cgcgcacgac ttccagcata cgttgccgtg gtgcgcttaa
    14221 tcgcccttgc aacatatcgc aaaccaccag cgcaccagca ttaataaacg gattacgcgg
    14281 aataccctgc tccatttcca gttgcactaa agaattgaac ggtgatccag acggatcttt
    14341 gccgacgcgt tgccagattt cctcttcgga gtaatgacgc atggcgacaa cgagactcag
    14401 cactttggaa atagactgaa tggaaaaacg ttcttgcgcg tctccggcct gaaaaagctg
    14461 tccgtcaacg gtacagatag caatgcccaa tcgggaaccg tctactgtag ccagcgccgg
    14521 aatataatcc gcgactttac cctgaccaat gagcggccgc acttgccgca agatgttttc
    14581 taaaattgca ttatccatgg cgactgccac tttctactcc tggaccgcag gtctgaaaag
    14641 acctgcgagt atatcagagc tgaatatgtc gcgtcagatc cggtctttcc acaccgtctg
    14701 gatattacag aattcgtgta agccgaaatg ggaaagctca cgaccaaagc cactcttttt
    14761 cacgccacca aaggccactc gcgcgtcgct ggcacaataa ccattgataa acaccccacc
    14821 gcattccaga cgtgccgcca tctgtctggc ctgtgtttcg tcggtggtaa aaatggtcgc
    14881 tgaaaggccg aactcactat cattagccag ttccagcgca tgttctgcat ctttcgcaac
    14941 ggtgattgcc gcaacagggc caaacatttc ttcccgaaac gcggtcattt ctggggtaac
    15001 attcgccaga accgttggcg gatagtagtt acctgcccca gccatctttt ccccgcccag
    15061 taacaaacgc gcaccctgcg ccagggtttt ctccacctga tgatgcagct catcacgtaa
    15121 atcaaaacga gccattggtc cgagagcatt ctcttcatca cggggatcgc ccattttcaa
    15181 ggctgccgca gctgccacaa aacgttcggt aaatgcagaa gcaattccct cttcgataat
    15241 aaagcgtttt gccgctgcac aaacctgtcc ggtattctga taacgtccgg ctaccgccgc
    15301 tttaaccgcc agttccagat cggcatcgtt aagcacaata aatggatccg aaccgcccag
    15361 ttccagtacg cattttttca gtgccgctcc agcctgtgcg ccaatagccg ctcccgcacg
    15421 aacacttccg gtcaccgtga cagcagcaat gcgcgaatct ttaatcattt gactgacacc
    15481 gtcgttgtcg gcattcagcc agccatatac gccttgcggg attcccgcat ctttaaacac
    15541 ctgggcaatg agctgagcac agcccatcac attcggcgca tgtttaagta agtagccgtt
    15601 acctgcaaga atgatgggaa ccgcgccacg catcacctgc cataacggaa agttccacgg
    15661 cataatcgcc agaatcgtcc ccaacggtcg atactcaata actgcctgct gattttccac
    15721 cagcgtaggt tccgccttca gcattgccgg accatgttct gcataccagt cacacaaatt
    15781 cgccgatttc gccacttcag cgcgcgcctg attgattggt ttgcccattt cacgggtgat
    15841 catttgcgcc atttcttcgc tacgggcgcg cagagcctta ccgataccac gcagtttttc
    15901 agcacgataa tctatatttg tctcgcgcca gtcgcgaaag cctgctgccg ccagctgaag
    15961 tgcgttttcg atatcgttag cgccagccca cggcagcaca gaaagttgtt cacccgtggc
    16021 aggatttatt gaaattgcat gagttgccgg agtaatggtc atcggggtat ctcctttatg
    16081 agtcatggta tgaagatacg cagatttact cttgctttaa aatgaataat attaagccac
    16141 ttattcacga atcgagaatg ttatggatct gacccaactg gagatgttca acgcggttgc
    16201 cgaagctggc agcattaccc aggctgcagc aaaagtgcat cgcgtaccgt cgaatctcac
    16261 tacccgtcta cgccagctgg aaacagaact gggggtagat ctgtttattc gcgagaatca
    16321 gcgtttacgt ctctctcctg ccgggcataa ctttttacgc tatagccaac aaattctcgc
    16381 gttagtggat gaagcgcgga gcgttgtcgc tggcgatgag ccgcaaggtt tattttctct
    16441 tggttcgctg gaaagcaccg cagcagtgcg cattccggcc acgctggcgg aattcaacca
    16501 tcgttatccc aaaattcagt tttcactttc caccggccct tccggcacta tgctggaagg
    16561 agtgctggag ggaaaactga atgcggcatt tattgatggg cccattaacc atactgccat
    16621 tgacgggata ccggtatacc gcgaggagct gatgatcgtc acgccacaag gtcatgcgcc
    16681 agtaatccgc gccagtcagg ttaacggcag taacatttat gccttccgcg ccaattgttc
    16741 gtatcgtcgc cacttcgaga gctggtttca tgctgacggt gccgctccgg gaactattca
    16801 cgagatggag tcgtatcacg ggatgttggc ctgtgtggtc gcaggagcag gcattgcgct
    16861 tattccacgc tctatgctgg aaagtatgcc ggggcatcac caggttgaag cgtggccgtt
    16921 agctgagcaa tggcgttggt taacaacctg gctggtctgg cgtcgcggtg cgaaaacgcg
    16981 tccgcttgag gcatttattc aactactgga tgtgcctgac tcggcaaaac agggttacca
    17041 atgagctatt tttgatagtt cttgcagctc aaatgcccta tatagtgatg ttccgatgac
    17101 ttatgactat atggggcaaa tatggttacg ccagtaagca tcagtaatta catatctctt
    17161 cctgatgatt ttcccgtacg taatattgca cctcaggtaa aagaagtttt aaaggacttt
    17221 attgatgcgc taagtacaat aatatgtgat gaagagtggc gcacgtcttt aaacatcaac
    17281 tcagccacaa aaaagatatt taataacctt gataacttat catatattca gaggacttct
    17341 tttcggggta acgacacgct atacaa
//
LOCUS       DAZKHF020000080        17193 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2201S31241
            SAMN43267800-rid25106413.denovo.080, whole genome shotgun sequence.
ACCESSION   DAZKHF020000080 DAZKHF020000000
VERSION     DAZKHF020000080.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267800
            Sequence Read Archive: SRR30307263
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 17193)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17193)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 17193)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350581.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350581.2
            Genome Coverage       :: 151x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:21:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,652
            CDSs (total)                      :: 5,557
            Genes (coding)                    :: 5,320
            CDSs (with protein)               :: 5,320
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 237
            CDSs (without protein)            :: 237
            Pseudo Genes (ambiguous residues) :: 0 of 237
            Pseudo Genes (frameshifted)       :: 102 of 237
            Pseudo Genes (incomplete)         :: 130 of 237
            Pseudo Genes (internal stop)      :: 52 of 237
            Pseudo Genes (multiple problems)  :: 41 of 237
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17193
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267800-rid25106413.denovo.080"
                     /serotype="O 26:H11"
                     /isolate="2201S31241"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-01"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            <1..578
                     /locus_tag="ACHOSC_RS11070"
                     /old_locus_tag="pgaptmp_002214"
     CDS             <1..578
                     /locus_tag="ACHOSC_RS11070"
                     /old_locus_tag="pgaptmp_002214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HGU4548144.1"
                     /translation="VTKTAIVGLLALFAGNAAATDGEIVFDGEILKSACEINDSDKKI
                     EVALGHYNAEQFRSVGDRSPKIPFTIPLVNCPVTGWEHDNGNVEASFRLWLETRDNGT
                     VPNFPNLAKVGSFAGTAATGVGIRIDDAESGNLMPLNAMGNDNTVYQIPADSNGIVNV
                     DLIAYYVSTVEASEITPGEADAVVNVTLDYR"
     gene            658..1353
                     /locus_tag="ACHOSC_RS11075"
                     /old_locus_tag="pgaptmp_002215"
     CDS             658..1353
                     /locus_tag="ACHOSC_RS11075"
                     /old_locus_tag="pgaptmp_002215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0061077 - chaperone-mediated protein
                     folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone"
                     /protein_id="HGU4548145.1"
                     /translation="MSKRTFAVIITLLCSFCIGQALAGGIVLQRTRVIYDASRKEAAL
                     PVANKGAETPYLLQSWVDNIDGTSRAPFIITPPLFRLEAGDDSSLRIIKTADNLPENK
                     ESLFYINVRAIPAKKKSDDVNANELTLVFKTRIKMFYRPAHLKGRVNDAWTSLEFKRS
                     DHSLNIYNPTEYYVVFAGLAVDKTDLTSKIEYIAPGEHKQLPLPASGGKNVKWAAIND
                     YGGSSGTETRPLQ"
     gene            1383..3899
                     /locus_tag="ACHOSC_RS11080"
                     /old_locus_tag="pgaptmp_002216"
     CDS             1383..3899
                     /locus_tag="ACHOSC_RS11080"
                     /old_locus_tag="pgaptmp_002216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015473 - fimbrial usher porin activity
                     [Evidence IEA];
                     GO_process: GO:0009297 - pilus assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbria/pilus outer membrane usher protein"
                     /protein_id="HGU4548146.1"
                     /translation="MPQRHHQGHKRTPKQLALIIKRCLPMVLTGSGMLCTTANAEEYY
                     FDPIMLETTKSGMQTTDLSRFSKKYAQLPGTYQVDIWLNKKKVSQKKITFTANAEQLL
                     QPQFTVEQLRELGIKVDEIPALAEKDDDSVINSLEQIIPGTAAEFDFNHQRLNLSIPQ
                     IALYRDARGYVSPSRWDDGIPTLFTNYSFTGSDNRYRQGNRSQRQYLNMQNGANFGPW
                     RLRNYSTWTRNDQASSWNTISSYLQRDIKALKSQLLLGESATSGSIFSSYTFTGVQLA
                     SDDNMLPNSQRGFAPTVRGIANSSAIVTIRQNGYVIYQSNVPAGAFEINDLYPSSNSG
                     DLEVTIEESDGTQRRFIQPYSSLPMMQRPGHLKYSATAGRYRADANSDSKEPEFAEAT
                     AIYGLNNTFTLYGGLLGSEDYYALGIGIGGTLGALGALSMDINRADTQFDNRHSFHGY
                     QWRTQYIKDIPETNTNIAVSYYRYTNDGYFSFDEANTRNWDYNSRQKSEIQFNISQTI
                     FDGVSLYASGSQQDYWGNNDKNRNISVGVSGQQWGIGYSLNYQYSRYTDQNNDRALSL
                     NLSIPLERWLPRSRVSYQMTSQKDRPTQHEMRLDGSLLDDGRLSYSLEQNLDDDNNHN
                     SSLNASYRSPYGTFSAGYSYGNDSSQYNYGVTGGVVIHPHGVTLSQYLGNAFALIDAN
                     GASGVRIQNYPGIATDPFGYAVVPYLTTYQENRLSVDTTQLPDNVDLEQTTQFVVPNR
                     GAMVAARFNANIGYRVLVTVSDRNGKPLPFGALASNDETGQQSIVDEGGILYLSGISS
                     KSQSWTVRWGNQADQQCQFAFSTPDSEPTTSVLQGTAQCH"
     gene            3910..5001
                     /locus_tag="ACHOSC_RS11085"
                     /old_locus_tag="pgaptmp_002217"
     CDS             3910..5001
                     /locus_tag="ACHOSC_RS11085"
                     /old_locus_tag="pgaptmp_002217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HGU4548147.1"
                     /translation="MKRAPLITGLLLISTSCAYASSGGCGADSTSGATNYSSVVDDVT
                     VNQTDNVTGREFTSATLSSTNWQYACTCSAGKAVKLVYMVSPVLTTTGHQAGYYKLND
                     SLDIKTTLQANDIPGLVTDQTVSVNTRFTQIKSNTVYSAATQTGVCQGDTSRYGPVNI
                     GANTTFTLYVTKPFLGSMTIPKTDIAVIKGAWVDGMGSPSTGDFHDLVKLSIQGNLTA
                     PQSCKINQGDVIKVNFGFINGQKFTTRNAMPDGFTPVDFDITYDCGDTSKIKNSLQMR
                     IDGTTGVVDQYNLVARRRSSDNAPDVGIRIENLGGGVANIPFQNGILPVDPSGHGTIN
                     MRAWPVNLVGGELETGKFQGTATITVIVR"
     gene            complement(5044..5904)
                     /gene="rsmI"
                     /locus_tag="ACHOSC_RS11090"
                     /old_locus_tag="pgaptmp_002218"
     CDS             complement(5044..5904)
                     /gene="rsmI"
                     /locus_tag="ACHOSC_RS11090"
                     /old_locus_tag="pgaptmp_002218"
                     /EC_number="2.1.1.198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (cytidine(1402)-2'-O)-methyltransferase"
                     /protein_id="HGU4548148.1"
                     /translation="MKQHQSADNSQGQLYIVPTPIGNLADITQRALEVLQAVDLIAAE
                     DTRHTGLLLQHFGINARLFALHDHNEQQKAETLLAKLQEGQNIALVSDAGTPLINDPG
                     YHLVRTCREAGICVVPLPGPCAAITALSAAGLPSDRFCYEGFLPAKSKGRRDALKAIE
                     AEPRTLIFYESTHRLLDSLEDIVAVLGESRYVVLARELTKTWETIHGAPVGELLAWVK
                     EDENRRKGEMVLIIEGHKAQEEDLPADALRTLALLQAELPLKKAAALAAEIHGVKKNA
                     LYKYALEQQG"
     gene            5968..8004
                     /gene="lpoA"
                     /locus_tag="ACHOSC_RS11095"
                     /old_locus_tag="pgaptmp_002219"
     CDS             5968..8004
                     /gene="lpoA"
                     /locus_tag="ACHOSC_RS11095"
                     /old_locus_tag="pgaptmp_002219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein activator LpoA"
                     /protein_id="HGU4548149.1"
                     /translation="MVPSTFSRLKAARCLPVVLAALIFAGCGTHTPDQSTAYMQGTAQ
                     ADSAFYLQQMQQSSDDTRINWQLLAIRALVKEGKTGQAVELFNQLPQELNDSQRREKT
                     LLAVEIKLAQKDFAGAQNLLAKITPADLEQNQQARYWQAKIDASQGRPSIDLLRALIA
                     QEPLLGAKEKQQNIDATWQALSSMTQEQANTLVINADENILQGWLDLQRVWFDNRNDP
                     DMMKAGIADWQKRYPNNPGAKMLPTQLVNVKAFKPASTNKIALLLPLNGQAAVFGRTI
                     QQGFEAAKNIGTQPVAAQVAAAPAADVAEQPQPQTVDGVASPAQASVSDLTGEQPAAQ
                     PAPVSTPAATTTAVSAPANPSAELKIYDTSSQPLSQILSQVQQDGASIVVGPLLKNNV
                     EELLKSNTPLNVLALNQPENIENRVNICYFALSPEDEARDAARHIRDQGKQAPLVLIP
                     RSSLGDRVANAFAQEWQKLGGGTVLQQKFGSTSELRAGVNGGSGIALTGTPITPRATT
                     DSGMTTNNPTLQTTPTDDQFTNNGGRVDAVYIVATPGEIAFIKPMIAMRNGSQSGATL
                     YASSRSAQGTAGPDFRLEMEGLQYSEIPMLAGGNLPLMQQALSAVNNDYSLARMYAMG
                     VDAWSLANHFSQMRQVQGFEINGNTGSLTANPDCVINRKLSWLQYQQGQVVPAS"
     gene            7962..8357
                     /locus_tag="ACHOSC_RS11100"
                     /old_locus_tag="pgaptmp_002220"
     CDS             7962..8357
                     /locus_tag="ACHOSC_RS11100"
                     /old_locus_tag="pgaptmp_002220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YraN family protein"
                     /protein_id="HGU4548150.1"
                     /translation="MATVPTRSGSPRQLTTKQTGDAWEVQARRWLEGKGLRFVAANVN
                     ERGGEIDLIMREGRTTVFVEVRYRRSALYGGAAASVTRSKQHKLLQTARLWLARHNGS
                     FDTVDCRFDVVAFTGNEVEWIKDAFNDHS"
     gene            8377..8967
                     /gene="diaA"
                     /locus_tag="ACHOSC_RS11105"
                     /old_locus_tag="pgaptmp_002221"
     CDS             8377..8967
                     /gene="diaA"
                     /locus_tag="ACHOSC_RS11105"
                     /old_locus_tag="pgaptmp_002221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001483830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaA initiator-associating protein DiaA"
                     /protein_id="HGU4548151.1"
                     /translation="MQERIKACFTESIQTQIAAAEALPDAISRAAMTLVQSLLNGNKI
                     LCCGNGTSAANAQHFAASMINRFETERPSLPAIALNTDNVVLTAIANDRLHDEVYAKQ
                     VRALGHAGDVLLAISTRGNSRDIVKAVEAAVTRDMTIVALTGYDGGELAGLLGPQDVE
                     IRIPSHRSARIQEMHMLTVNCLCDLIDNTLFPHQDV"
     gene            8977..9552
                     /gene="dolP"
                     /locus_tag="ACHOSC_RS11110"
                     /old_locus_tag="pgaptmp_002222"
     CDS             8977..9552
                     /gene="dolP"
                     /locus_tag="ACHOSC_RS11110"
                     /old_locus_tag="pgaptmp_002222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006970 - response to osmotic stress
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="division/outer membrane stress-associated
                     lipid-binding lipoprotein"
                     /protein_id="HGU4548152.1"
                     /translation="MKALSPIAVLISALLLQGCVAAAVVGTAAVGTKAATDPRSVGTQ
                     VDDGTLEVRVNSALSKDEQIKKEARINVTAYQGKVLLVGQSPNAELSARAKQIAMGVD
                     GANEVYNEIRQGQPIGLGEASNDTWITTKVRSQLLTSDLVKSSNVKVTTENGEVFLMG
                     LVTEREAKAAADIASRVSGVKRVTTAFTFIK"
     gene            complement(9666..10706)
                     /locus_tag="ACHOSC_RS11115"
                     /old_locus_tag="pgaptmp_002223"
     CDS             complement(9666..10706)
                     /locus_tag="ACHOSC_RS11115"
                     /old_locus_tag="pgaptmp_002223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="permease"
                     /protein_id="HGU4548153.1"
                     /translation="MTGQSSSQAATPIQWWKPALFFLVVIAGLWYVKWQPYYGKAFTA
                     AETHSIGKSILAQADANPWQAALDYAMLYFLAVWKAAVLGVILGSLIQVLIPRDWLLR
                     TLGQSRFRGTLLGTLFSLPGMMCTCCAAPVAAGMRRQQVSMGGALAFWMGNPVLNPAT
                     LVFMGFVLGWGFAAIRLVAGLVMVLLIATLVQKWVRETPQTQAPVEIDIPEAQGGFFS
                     RWGRALWTLFWSTIPVYILAVLVLGAARVWLFPHADGAVDNSLMWVMAMAVAGCLFVI
                     PTAAEIPIVQTMMLAGMGTAPALALLMTLPAVSLPSLIMLRKAFPAKALWLTGAMVAV
                     SGVIVGGLALLF"
     gene            complement(10779..11414)
                     /locus_tag="ACHOSC_RS11120"
                     /old_locus_tag="pgaptmp_002224"
     CDS             complement(10779..11414)
                     /locus_tag="ACHOSC_RS11120"
                     /old_locus_tag="pgaptmp_002224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312060.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-binding protein"
                     /protein_id="HGU4548154.1"
                     /translation="MSQVLITGATGLVGGHLLRMLINEPKVNAIAAPTRRPLGDMPGV
                     FNPHDPQLTDALAQVTDPIDIVFCCLGTTRREAGSKEAFIHADYTLVVDTALTGRRLG
                     AQHMLVVSAMGANAHSPFFYNRVKGEMEEALIAQNWPKLTIARPSMLLGDRSKQRMNE
                     TLFAPLFRLLPGNWKSIDARDVARVMLAEAMRPGHEGVTILSSSELRKRAE"
     gene            11542..12060
                     /gene="yhbO"
                     /locus_tag="ACHOSC_RS11125"
                     /old_locus_tag="pgaptmp_002225"
     CDS             11542..12060
                     /gene="yhbO"
                     /locus_tag="ACHOSC_RS11125"
                     /old_locus_tag="pgaptmp_002225"
                     /EC_number="3.5.1.124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417622.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein/nucleic acid deglycase"
                     /protein_id="HGU4548155.1"
                     /translation="MSKKIAVLITDEFEDSEFTSPADEFRKAGHEVITIEKQAGKTVK
                     GKKGEASVTIDKSIDEVTPAEFDALLLPGGHSPDYLRGDNRFVTFTRDFVNSGKPVFA
                     ICHGPQLLISADVIRGRKLTAVKPIIIDVKNAGAEFYDQEVVVDKDQLVTSRTPDDLP
                     AFNREALRLLGA"
     gene            complement(12040..12483)
                     /locus_tag="ACHOSC_RS11130"
                     /old_locus_tag="pgaptmp_002226"
     CDS             complement(12040..12483)
                     /locus_tag="ACHOSC_RS11130"
                     /old_locus_tag="pgaptmp_002226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhbP family protein"
                     /protein_id="HGU4548156.1"
                     /translation="METLITISRWLAKQHVVTWCVQQEGELWCANAFYLFDAQKVAFY
                     ILTEEKTRHAQMSGPQAAVAGTVNGQPKTVALIRGVQFKGEIRRLEGEESDLARKAYN
                     RRFPVARMLSAPVWEIRLDEIKFTDNTLGFGKKMIWLRGSGTEQA"
     gene            12534..12836
                     /gene="yhbQ"
                     /locus_tag="ACHOSC_RS11135"
                     /old_locus_tag="pgaptmp_002227"
     CDS             12534..12836
                     /gene="yhbQ"
                     /locus_tag="ACHOSC_RS11135"
                     /old_locus_tag="pgaptmp_002227"
                     /EC_number="3.1.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708956.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA damage response exodeoxyribonuclease YhbQ"
                     /protein_id="HGU4548157.1"
                     /translation="MTPWFLYLIRTADNKLYTGITTDVERRYQQHQSGKGAKALRGKG
                     ELTLAFSAPVGDRSLALRAEYRVKQLTKRQKERLVAEGAGFAELLSSLQTPEIKSD"
     gene            complement(12823..13326)
                     /locus_tag="ACHOSC_RS11140"
                     /old_locus_tag="pgaptmp_002228"
     CDS             complement(12823..13326)
                     /locus_tag="ACHOSC_RS11140"
                     /old_locus_tag="pgaptmp_002228"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HGU4548158.1"
                     /translation="MLIRVEIPIDAPGIDALLRRSFESDAEAKLVHDLREDGFLTLGL
                     VATDDEGQVIGYVAFSPVDVQGEDLQWVGMAPLAVDEKYRGQGLARQLVYEGLDSLNE
                     FGYAAVVTLGDPALYSRFGFELAAHHDLRCRWPGTESAFQVHRLADDALNGVTGLVEY
                     HEHFNRF"
     gene            complement(13320..13844)
                     /locus_tag="ACHOSC_RS11145"
                     /old_locus_tag="pgaptmp_002229"
     CDS             complement(13320..13844)
                     /locus_tag="ACHOSC_RS11145"
                     /old_locus_tag="pgaptmp_002229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SCP2 domain-containing protein"
                     /protein_id="HGU4548159.1"
                     /translation="MLDKLRSRIVHLGPSLLSVPVKLTPFALKRQVLEQVLSWQFRQA
                     LDDGELEFLEGRWLSIHVRDIDLQWFTSVVNGKLVVSQNAQADVSFSADASDLLMIAA
                     RKQDPDTLFFQRRLVIEGDTELGLYVKNLMDAIELEQMPKALRMMLLQLADFVEAGMK
                     TAPETKQTSVGEPC"
     gene            14053..15048
                     /locus_tag="ACHOSC_RS11150"
                     /old_locus_tag="pgaptmp_002230"
     CDS             14053..15048
                     /locus_tag="ACHOSC_RS11150"
                     /old_locus_tag="pgaptmp_002230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017457364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase U32 family protein"
                     /protein_id="HGU4548160.1"
                     /translation="MELLCPAGNLPALKAAIENGADAVYIGLKDDTNARHFAGLNFTE
                     KKLQEAVSFVHQHRRKLHIAINTFAHPDGYARWQRAVDMAAQLGADALILADLAMLEY
                     AAERYPHIERHVSVQASATNEEAINFYHRHFDVARVVLPRVLSIHQVKQLARVTPVPL
                     EVFAFGSLCIMSEGRCYLSSYLTGESPNTVGACSPARFVRWQQTPQGLESRLNEVLID
                     RYQDGENAGYPTLCKGRYLVDGERYHALEEPTSLNTLELLPELMAANIASVKIEGRQR
                     SPAYVSQVAKVWRQAIDRCKADPQNFIPQSAWMETLGSMSEGTQTTLGAYHRKWQ"
     gene            15057..15935
                     /locus_tag="ACHOSC_RS11155"
                     /old_locus_tag="pgaptmp_002231"
     CDS             15057..15935
                     /locus_tag="ACHOSC_RS11155"
                     /old_locus_tag="pgaptmp_002231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312067.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006744 - ubiquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="U32 family peptidase"
                     /protein_id="HGU4548161.1"
                     /translation="MKYSLGPVLWYWPKETLEDFYQQAATSSADVIYLGEAVCSKRRA
                     TKVGDWLEMAKSLAGSGKQIVLSTLALVQASSELGELKRYVENGEFLIEASDLGVVNM
                     CAERKLPFVAGHALNCYNAVTLKILLKQGMMRWCMPVELSRDWLVNLLNQCDELGIRN
                     QFEVEVLSYGHLPLAYSARCFTARSEDRPKDECETCCIKYPNGRNVLSQENQQVFVLN
                     GIQTMSGYVYNLGNELASMQGLVDVVRLSPQGTDTFAMLDAFRANENGAAPLPLTANS
                     DCNGYWRRLAGLELQA"
     gene            16141..17148
                     /locus_tag="ACHOSC_RS11160"
                     /old_locus_tag="pgaptmp_002232"
     CDS             16141..17148
                     /locus_tag="ACHOSC_RS11160"
                     /old_locus_tag="pgaptmp_002232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HGU4548162.1"
                     /translation="MTDKTIAFSLLDLAPIPEGSSAREAFSHSLDLACLAEKRGYHRY
                     WLAEHHNMTGIASAATSVLIGYLAANTTTLHLGSGGVMLPNHSPLVIAEQFGTLNTLY
                     PGRIDLGLGRAPGSDQRTMMALRRHMSGDIDNFPRDVAELVDWFDARDPNPHVRPVPG
                     YGEKIPVWLLGSSLYSAQLAAQLGLPFAFASHFAPDMLFQALHLYRSNFKPSARLEKP
                     YAMVCINIIAADSNRDAEFLFTSMQQAFVKLRRGETGQLPPPIQNMDQFWSPSEQYGV
                     QQALSMSLVGDKAKVRHGLQSILRETDADEIMVNGQIFDHQARLHSFELAMDVKEELL
                     G"
BASE COUNT         4329 a         4560 c         4351 g         3953 t
ORIGIN      
        1 aagttacaaa aacagctatt gtgggcttac ttgcgctttt cgcaggtaat gccgctgcaa
       61 ccgatggtga aatagttttt gatggcgaga ttttaaaatc cgcttgtgaa atcaatgact
      121 ctgataagaa aattgaagtt gctcttggtc actataatgc cgaacagttt cgttctgttg
      181 gcgatcgtag cccaaaaatt ccattcacta ttcctttagt taactgccca gtgactgggt
      241 gggagcacga taacggcaac gttgaagcgt ctttccgttt atggctggaa acgcgggata
      301 atggcaccgt ccctaacttc cctaatctgg caaaagtggg ctcttttgct ggcacagcgg
      361 caacaggtgt gggtatccgt attgacgatg cggaaagcgg aaaccttatg ccactgaatg
      421 ctatggggaa tgataacacg gtttatcaga tcccagcgga ctccaatggt attgtcaatg
      481 ttgacctcat cgcttactac gtatcaaccg ttgaagcatc agaaatcacc ccaggggaag
      541 cagacgctgt cgttaacgta acgctggatt accgttaata cgttacggcg ttatctgacc
      601 tttcagataa cgcccttttc cttcctcttt ctcgttgtat caggttgaaa aataactatg
      661 tcaaaacgaa cattcgcggt gataataacc ttgttgtgta gcttctgtat cggccaggcg
      721 cttgcagggg gtatcgtttt acagcgaacg cgagtgatct atgatgccag ccgcaaagag
      781 gctgcgttac ctgtcgcaaa caaaggcgca gaaacgcctt atttactgca atcatgggta
      841 gataatatag atggtacaag ccgtgcccca tttattataa ccccaccgct atttcgtctt
      901 gaagccggcg atgactcatc actgcgaatt attaaaacag ctgataacct gcctgaaaat
      961 aaagagtcgc tgttctacat taatgttcgt gccattccag caaagaaaaa atcagatgat
     1021 gttaatgcta acgagttgac gctggtattt aaaacacgga tcaaaatgtt ttatcgcccc
     1081 gcacacctga agggacgggt aaacgatgcg tggacatcac tggaatttaa acgtagtgac
     1141 cattcactca atatatataa cccaactgaa tattacgtcg tatttgccgg actggcagtc
     1201 gataaaaccg atctcacaag caaaattgaa tatatcgcgc ccggagaaca taaacagtta
     1261 ccacttcctg catctggcgg aaagaacgtg aaatgggctg caatcaatga ttatggcggc
     1321 agttccggga cagaaactcg tccactgcaa taacaaatat aaaaaacaca ggtcatcagg
     1381 gaatgccaca acgacaccac cagggacata aacgcacacc gaaacagttg gcgctcatca
     1441 tcaaacgctg tttgccgatg gtgctcactg gcagcggcat gctttgcact accgctaacg
     1501 ccgaagagta ttatttcgac cccattatgc tggaaactac aaaaagtggt atgcaaacaa
     1561 ctgatctgtc acgtttttca aaaaaatacg cacaactacc aggaacttat caggttgata
     1621 tctggctgaa taaaaagaag gtttcacaga aaaaaattac atttaccgcc aatgcagagc
     1681 aacttctgca gccacagttt acggtagaac aactacgtga gctgggtatt aaggtggatg
     1741 aaatcccggc gctggctgaa aaagatgacg atagcgtgat caactcgctt gaacaaatca
     1801 ttcccggtac agctgctgaa tttgatttca atcatcagcg acttaatttg agcattcccc
     1861 aaattgcact gtaccgtgat gcaagaggtt acgtctcccc ttctcgttgg gacgatggta
     1921 taccaacgct gtttaccaac tactcgttta caggttctga taaccgttac cgccagggca
     1981 atcgtagcca acgacagtac ctgaatatgc aaaatggtgc taattttggc ccctggcgat
     2041 tacgtaacta ttctacgtgg acacgcaacg atcaggcgtc aagctggaac actatcagta
     2101 gttatttaca acgtgatatc aaggcgttga agtctcagtt gcttctggga gaaagcgcca
     2161 ccagcggcag tattttttcc agctacacct ttactggcgt gcaactcgct tccgacgata
     2221 atatgttgcc aaacagccag cgcggatttg ccccaacggt acgcggtatc gcaaacagta
     2281 gtgcaatcgt gactatcagg caaaatggtt atgtgatcta tcaaagcaac gtgccagcgg
     2341 gtgcctttga aattaacgat ctctacccct cttccaacag cggcgattta gaagtcacga
     2401 ttgaagaaag tgacggtacg caacgtcgct ttatccagcc ttattcttca ttacccatga
     2461 tgcagcgacc tgggcatcta aaatatagcg cgaccgctgg acgctatcgc gctgatgcaa
     2521 acagtgatag caaggaaccc gaatttgctg aagccacggc aatatatggt ttgaataata
     2581 cttttacgct gtatggcggc ctgctcggtt ctgaagatta ttatgcgctg gggatcggta
     2641 tcggcggcac acttggcgca ctgggcgcgt tgtcgatgga tatcaacaga gctgacaccc
     2701 aattcgataa ccggcactct tttcatggct atcaatggcg tacgcagtac atcaaagata
     2761 tcccggaaac caacaccaat atcgctgtta gctactatcg ctataccaac gatggctatt
     2821 ttagttttga tgaagccaat acccgtaatt gggactataa cagtcgccaa aaaagtgaaa
     2881 ttcaattcaa catcagccag acaatatttg atggggtaag tctgtatgcc tccggttcgc
     2941 agcaagacta ttggggcaat aacgataaaa acaggaatat ctcagttggg gtttccggtc
     3001 agcaatgggg aattggttac agcctgaatt atcaatacag tcgctacacg gatcaaaata
     3061 atgaccgcgc actctctttg aatctcagta ttccgttaga acgctggtta ccgcgtagcc
     3121 gggtttccta tcagatgacc agccagaaag atcgcccaac ccaacatgaa atgcgtcttg
     3181 atggctcact gctggatgat ggtcgcctga gctatagcct ggaacaaaat ctggatgacg
     3241 ataacaacca taacagtagc ctgaacgcca gttaccgttc accttatggc accttcagtg
     3301 ccggatacag ctacggtaat gacagcagcc aatacaatta cggcgttacc ggcggcgtgg
     3361 ttatccatcc tcatggcgtg acgctctcgc aatatctggg caacgctttt gcgcttatcg
     3421 atgctaatgg ggcttctggc gtgaggatac aaaactatcc ggggattgct actgatccct
     3481 ttggctatgc agtggttcct tatctcacga cttaccagga aaaccgtctc tcggtagata
     3541 ctacgcagct gcccgataac gtcgatcttg agcaaacaac acagtttgtg gtgcccaaca
     3601 gaggtgcaat ggtagcggcg cgtttcaacg ccaatatcgg ttatcgcgta cttgttacag
     3661 tcagcgatcg caacggtaaa ccgttgccct ttggcgctct tgccagcaac gatgagacgg
     3721 ggcaacaaag tatcgtcgat gagggcggca tactatatct ctctgggata tcgagtaaat
     3781 cacaaagctg gactgtacgc tggggaaatc aggcagatca acaatgtcag tttgctttta
     3841 gtacaccgga ttcagaacca acaacctctg tattacaagg cacggcgcag tgccattaag
     3901 gataaaaaaa tgaaaagagc gcctcttata acaggacttt tgttgatatc cacatcctgc
     3961 gcttatgcct cctcaggagg gtgtggagct gacagcacta gcggtgcgac aaattacagc
     4021 agtgtggttg atgatgttac ggtgaaccag acagataacg tgacaggacg ggagtttacc
     4081 tctgccacgc taagtagcac taactggcaa tacgcctgta cctgctctgc gggtaaggca
     4141 gttaaacttg tctatatggt cagccctgta cttaccacca ctggacatca agcaggatat
     4201 tacaaactca atgatagtct ggatattaaa accacattac aggcgaatga cattccagga
     4261 ctcgtgactg accagaccgt ttctgttaac acccgattca cacagataaa aagcaacacc
     4321 gtatattctg ctgcaaccca aacgggtgtt tgccagggtg acacgtctcg ttatggaccc
     4381 gttaatattg gtgcaaatac cacctttacc ctgtatgtca ccaagccatt tctcggctcg
     4441 atgaccattc cgaaaacgga tattgccgtc attaaaggcg catgggtcga tggaatggga
     4501 agcccgtcta caggtgactt ccatgattta gtcaagttat cgattcaggg aaatctcacc
     4561 gccccacagt cgtgcaaaat taatcagggc gatgttatta aggttaattt tggattcatc
     4621 aatggtcaga agtttaccac ccgcaatgcc atgccagacg gttttactcc agtagacttt
     4681 gatatcactt atgactgtgg tgatacttca aagattaaaa actcgttgca aatgcgcatc
     4741 gacggtacaa ctggggtagt agaccagtac aacctggtcg ccagacgaag aagttcagac
     4801 aatgcgcccg atgtcggtat tcgtattgaa aatctcggcg gcggagttgc aaatattcct
     4861 tttcagaacg gtatccttcc cgttgatcct tccgggcatg gcaccatcaa catgcgcgcc
     4921 tggccagtta atctggtcgg tggtgagctg gaaacaggaa aatttcaggg caccgccacc
     4981 attaccgtca tcgtgcggta aaagaagacg ttgcgttttg attaacgcaa cgcttcggca
     5041 ctcttacccc tgctgctcca gcgcatactt atacagcgca tttttcttca cgccgtgaat
     5101 ttctgccgcc agcgccgccg cttttttcaa cggcagttct gcctgtagca gcgccagcgt
     5161 gcgcagggca tcggcgggta agtcttcttc ctgtgcttta tgaccttcga taatcagcac
     5221 catttcgcct ttgcgacggt tttcatcttc ttttacccac gccagcagct cgccaacggg
     5281 cgcgccgtga atggtttccc aggttttggt cagctcacgc gccagaacca cgtagcggga
     5341 ttcgcctaat accgcaacga tatcttccag gctatctaac agacggtggg tggattcata
     5401 aaaaatcagc gtgcgcggct ccgcttcaat ggcttttagc gcatcacggc ggccttttga
     5461 tttggcaggt aaaaagcctt cgtaacagaa acggtcagag ggtaaacccg cggcgcttaa
     5521 cgcagtgata gcagcacacg gcccgggtag cggcaccacg caaatccccg cttcacggca
     5581 ggtacgcacc agatggtagc caggatcgtt aattagcggc gttccggcat cggaaaccag
     5641 cgcaatgttc tgcccttctt gcagcttcgc cagcagcgtt tcggcttttt gttgttcgtt
     5701 atggtcgtgc agcgcaaaca accgggcatt aatcccaaaa tgttgcagca ataaaccggt
     5761 gtgacgagta tcctcggcgg caatcagatc aacggcctgt aatacctcta acgcacgctg
     5821 ggtgatatcc gccagattgc cgattggcgt cggtacaatg taaagctggc cctgagaatt
     5881 atccgccgat tggtgttgtt tcattgtgtc gtccgtattg ccgatttaat attgagcatt
     5941 gcgtaaaaaa atatcactgg atacattatg gtaccctcaa cattttctcg tttgaaagcc
     6001 gcgcgttgtc tgcctgttgt tctggcagcc ctgattttcg ccggttgtgg cacccatact
     6061 cccgatcagt ccactgctta tatgcagggc acggcgcagg ctgattctgc cttttatctt
     6121 cagcagatgc agcaaagctc tgatgatacc aggatcaact ggcaattact cgccattcgt
     6181 gcactggtga aagaaggtaa aaccgggcag gcggttgagt tgtttaacca actaccgcaa
     6241 gaactgaacg attctcagcg tcgcgagaaa acactgctgg cggtagagat taaactggcg
     6301 cagaaagatt ttgctggcgc gcaaaacttg ctggcgaaaa tcacacctgc cgatttagaa
     6361 caaaaccagc aagcgcgtta ctggcaggca aaaatcgatg ccagccaggg gcgtccttcc
     6421 attgatttac tgcgcgcgtt aattgctcag gaaccgctgc tcggcgcgaa agaaaaacag
     6481 cagaatattg atgccacctg gcaggcgctc tcctccatga ctcaggaaca ggcgaatacg
     6541 ctggtgatca acgccgacga aaatattctg caaggctggc tggatctgca gcgcgtctgg
     6601 tttgataacc gtaacgatcc cgacatgatg aaagccggga tcgccgactg gcaaaaacgt
     6661 tatccgaaca atccgggcgc gaaaatgctg ccaacgcagt tggttaacgt aaaagcgttt
     6721 aaaccagcct cgaccaacaa aatcgccctg ctgttgccgc tgaatggcca ggcagcggta
     6781 tttggtcgca ctattcagca aggctttgaa gcggcgaaaa atatcggcac tcagccagtg
     6841 gcggctcagg tagctgccgc acctgccgca gacgtagctg aacaacctca gccgcaaacc
     6901 gtggatggcg ttgccagccc ggcacaagcc tcggttagcg atctgaccgg tgaacagcct
     6961 gcggcacagc cagcgcctgt aagcaccccg gcagcaacca ccacagcggt aagcgcgcca
     7021 gcgaatccat ccgcagaact gaaaatctac gatacctcat cacaaccact tagccagatc
     7081 ttaagccagg ttcagcagga tggcgcgagt attgtggttg gtccgttgct gaaaaataac
     7141 gttgaagagt tgcttaagag caacaccccg ctgaacgtgc tggcactgaa ccagccggag
     7201 aatatcgaaa accgcgtcaa tatttgttac ttcgcgcttt caccggaaga cgaagcgcgc
     7261 gatgcagcgc gtcatattcg tgaccagggt aaacaagcgc cgctggtgct gatcccacgc
     7321 agttcattgg gcgatcgcgt agccaatgcg tttgcgcagg agtggcagaa actgggcggc
     7381 ggcaccgttc tgcaacaaaa atttggttcc accagcgaat tacgcgcggg tgttaacggc
     7441 ggttctggta tcgctttaac gggtaccccg attactccca gagcgacaac cgactccggc
     7501 atgacgacca acaatccaac gctgcaaacc acgccaaccg atgaccagtt caccaataat
     7561 ggcggtcgtg tcgatgcggt gtacattgtg gcaacgccgg gtgaaatcgc ttttatcaaa
     7621 ccgatgatcg ccatgcgtaa cggtagccag agcggtgcaa cgctgtacgc cagctcccgc
     7681 agtgcgcaag ggaccgctgg cccggatttc cgactggaga tggaaggctt gcagtacagc
     7741 gaaatcccga tgctggcagg cggtaatcta ccgttaatgc agcaggcact cagcgcggtg
     7801 aataacgatt attcactggc tcgcatgtat gcgatgggcg tcgatgcctg gtcgctggca
     7861 aatcatttct cacaaatgcg ccaggttcag ggttttgaaa tcaacggtaa taccggaagc
     7921 ctgacggcaa acccggattg cgtgattaac aggaagttat catggctaca gtaccaacaa
     7981 ggtcaggtag tccccgccag ttaaccacca aacagaccgg cgatgcgtgg gaagtacaag
     8041 cgcgtcgctg gctggaaggc aaaggactgc gatttgtcgc cgctaacgtg aacgagcgtg
     8101 gcggcgagat cgatctgata atgcgtgaag gccggaccac cgtttttgtc gaggtacgct
     8161 atcgccgctc tgcgctttat ggcggcgcgg cagccagtgt gacccgcagc aaacaacaca
     8221 aattattaca gactgcccgc ttgtggctcg cgcgtcataa tgggagtttt gatactgtgg
     8281 attgccggtt cgatgtggta gccttcaccg ggaatgaggt tgagtggatt aaggatgcct
     8341 ttaatgacca ctcataatta aggtttaagg attagcgtgc aagaaagaat taaagcttgc
     8401 ttcactgaaa gcattcaaac tcaaattgcg gcggcagagg cgcttccgga tgccatctcc
     8461 cgtgcagcca tgacgctggt tcagtctctg ctcaatggca acaaaatcct ctgttgtggt
     8521 aatggaactt ccgctgccaa tgcacagcat tttgctgcca gcatgatcaa ccgtttcgaa
     8581 acggagcggc ccagcttacc tgccattgca ctaaatactg ataatgttgt cttaacggcg
     8641 attgccaacg atcgcttaca tgatgaagtg tatgcaaaac aggtgcgggc gctgggtcat
     8701 gcgggagatg tattgttagc catttccacc cgtggcaaca gccgcgatat tgtaaaagcg
     8761 gtagaagctg ccgttacgcg tgatatgact attgtggcat tgaccggcta tgacggcggc
     8821 gaacttgcag gtttgttagg gccacaagat gtggagatcc gcattccttc gcatcgtagt
     8881 gctcgcattc aggaaatgca tatgctgacg gtaaattgcc tgtgcgatct gatcgataac
     8941 acgcttttcc ctcaccagga tgtttaagga gaatacatga aggcattatc gccaatcgca
     9001 gtccttattt ccgcgctgct gttgcaaggt tgtgttgccg ctgccgtagt gggtaccgct
     9061 gctgtgggta ccaaagccgc aactgaccca cgcagtgtcg gcacccaggt ggacgatggt
     9121 accctggaag tgcgcgtgaa cagcgcattg tcgaaagacg aacagattaa gaaagaagcc
     9181 cgtattaatg tcacggcgta tcagggcaaa gtgctgctgg ttgggcagtc accaaacgct
     9241 gaactttcgg ctcgcgccaa acagattgct atgggcgtag acggtgccaa cgaagtgtat
     9301 aacgagattc gtcagggcca gccgattggt ctgggcgaag cgtctaacga tacgtggatc
     9361 accaccaaag tgcgttcgca gctcttaacc agcgacctgg tgaaatcgtc caacgtgaaa
     9421 gtgaccaccg aaaacggtga agtgttcctg atggggctgg tgactgaacg tgaagcgaaa
     9481 gcggcggcag atattgccag ccgggtgagc ggcgtaaagc gggtaaccac agcgtttacg
     9541 tttattaaat agcagcccct tgtaatgcct gatgcgacgc ttgccgcgtc ttatcaggcc
     9601 tacaacgatg caaaccgtag gtcggataag gcgtttacgc cgcatccgac atgttttccc
     9661 tcaaatcaga acaacagcgc cagcccgccg acaatcacac cagacactgc caccatcgcc
     9721 cctgtcagcc ataaggcttt cgctgggaac gctttgcgca gcataatcag tgacggcaaa
     9781 ctcaccgccg ggagcgtcat caacaatgcc agcgccggag cagttcccat acctgccagc
     9841 atcatcgttt gtacaatcgg aatttctgct gccgtgggaa tgacaaacaa gcatcctgct
     9901 accgccatcg ccatcaccca catcaggctg ttatcgacag caccatcggc atgggggaat
     9961 aaccagacgc gagcggcacc caacaccagt actgcaagga tgtaaaccgg gatcgtactc
    10021 cagaaaagcg tccatagcgc cctgccccag cggctaaaaa aaccgccctg tgcttccggt
    10081 atgtcaattt cgaccggtgc ctgcgtttgc ggtgtttcac gcacccattt ttgcaccagc
    10141 gtcgcaatca gcaacaccat caccagcccg gccaccagac gaatcgccgc aaaaccccag
    10201 ccgaggacaa accccataaa caccagcgtc gccgggttta acaccggatt gcccatccag
    10261 aatgccagcg caccgcccat cgacacctgt tggcgacgca ttcccgccgc gaccggagcc
    10321 gcacagcagg tacacatcat gcccggcagc gaaaacagcg ttcccagcag cgtgccgcga
    10381 aagcgcgatt gcccaagcgt acgcaacaac cagtcacgcg ggatcagcac ctgaatcaac
    10441 gaaccgagga tcacccccag cactgccgct ttccataccg cgaggaaata gagcatcgcg
    10501 taatccaacg ccgcctgcca cggattagca tccgcctgcg caaggataga tttaccgata
    10561 ctgtgggttt cggcagcagt aaacgctttg ccgtagtaag gttgccattt cacataccag
    10621 agaccggcaa tgacaacgag aaagaaaagc gcgggtttcc accactgaat gggcgttgcc
    10681 gcctgagatg aagactgacc agtcatagca ttccccagga ataagttatg ataattgagc
    10741 gcgtgaatat tacgctcact atcaattctt gggaataatt attcagctct tttgcgtaat
    10801 tctgaagagc ttaaaatcgt cacaccttca tgcccgggcc gcatcgcctc tgccagcatg
    10861 acccgcgcca cgtctctcgc atcaatggat ttccagttac cgggtaataa acggaacagc
    10921 ggcgcaaaaa gcgtttcgtt catccgctgt ttgctacgat cgcccagtaa catcgacggg
    10981 cgagcaatgg tcaatttcgg ccagttctgg gcgattaatg cttcttccat ctcccctttg
    11041 acgcggttat agaaaaacgg cgagtgggca ttggcaccca tagcactgac caccagcata
    11101 tgctgcgcgc ccagtcgccg cccggttaat gcggtatcca ctaccagcgt gtaatcggca
    11161 tgaataaacg cctctttgct ccccgcttct cgccgcgtgg tgccgagaca acaaaacaca
    11221 atgtcgatgg gatcggtgac ctgcgccagc gcgtcagtca gttgcggatc atggggatta
    11281 aacaccccgg gcatatcgcc caacggacgt cgcgtcggcg cggctatggc gttaactttc
    11341 ggttcgttaa tcaacatccg cagcaggtga ccgcccacca gccccgttgc gcctgtaatc
    11401 agtacctgac tcatcttcgc tcctttacag aattgtctgc cttgcgctcc acggctcatg
    11461 caccaggctt aatagaccat gaggtaatta tccccgattg tgggaaattc gcctcatcca
    11521 atgcaacaac gcggaggaag catgagtaag aaaattgccg ttttaatcac tgatgaattt
    11581 gaagattcag aatttacttc acccgcagac gagttccgta aagccggaca cgaagtgatt
    11641 accattgaaa aacaagcggg taaaacggtg aaaggcaaaa aaggagaagc cagcgtgacc
    11701 atcgataaat ccatcgatga agtgacgcca gcggagtttg atgccctgct gctaccgggc
    11761 ggccattcac cggattatct gcgtggcgac aaccgttttg tcacctttac ccgtgatttt
    11821 gtgaatagcg gcaaacctgt tttcgccatc tgtcacggtc cgcagctgct gatcagcgcc
    11881 gatgtgattc gggggcgcaa actgaccgcg gttaaaccga tcattattga tgttaaaaac
    11941 gcgggcgcgg aattttacga tcaggaagtc gtggtcgata aagatcagct ggttaccagc
    12001 cggacaccgg acgatctgcc agcgtttaac cgcgaggcgt tacgcctgct cggtgcctga
    12061 gccgcgtagc caaatcattt ttttaccaaa gcccagcgtg ttgtcggtga atttgatttc
    12121 atcgagccgg atttcccaca ccggggccga cagcattctg gcaaccggaa agcgacgatt
    12181 gtacgccttg cgcgcgaggt cgctttcctc accttccagc ctgcggatct cacctttaaa
    12241 ctgcacgccg cgaattaacg ctaccgtttt cggctgaccg tttaccgttc cggcaaccgc
    12301 agcctgcggc ccactcatct gcgcgtggcg cgttttttct tccgtcaaaa tgtagaaggc
    12361 gactttctgc gcatcaaaca gataaaaagc attagcgcac cagagttccc cttcctgctg
    12421 cacacaccag gtgacaacat gttgttttgc caaccaacgg ctgatggtga tgagtgtttc
    12481 cattgctgtt ctcttttata ctgtgggcct gaccttaaca tgcgacgcag acgatgacac
    12541 cctggtttct ttacttgatc cgtaccgccg acaataagct ttataccggg atcaccacgg
    12601 atgtcgaacg ccgctatcag cagcaccaaa gcggcaaagg ggcgaaagca ctgcgcggga
    12661 aaggagaact gacgctggcg ttttctgcgc cagtgggcga tcgttcgctg gcgttacggg
    12721 cggaatatcg cgttaagcaa ctgaccaaac ggcaaaaaga gcgtctggta gcggaaggcg
    12781 cagggtttgc agagctgtta agcagtctgc aaaccccgga gattaaaagc gattgaaatg
    12841 ctcgtgatac tcaaccagac cagttacgcc attcaacgcg tcatccgcta aacgatgtac
    12901 ctggaaggcg ctttcagtgc ctggccagcg gcaacgcagg tcgtgatgtg ccgccagttc
    12961 aaagccaaaa cggctgtaca gcgccggatc gcccagcgtc accactgcgg catagccgaa
    13021 ctcattaagc gaatcgagtc cttcatagac cagttggcgt gccagcccct gtccacggta
    13081 tttttcatca accgccaacg gtgccatgcc gacccattgc aggtcttcgc cctgcacatc
    13141 aaccggacta aatgccacat agccaatgac ctgaccttcg tcatctgtcg ccaccagccc
    13201 cagcgtcaga aagccatctt cacgcagatc gtgaaccagc ttcgcttccg catcactttc
    13261 gaatgagcga cgtagcaggg catcaatacc cggcgcatca atgggaattt ctactcgaat
    13321 tagcatggtt cacctaccga tgtctgtttg gtttcaggcg cggttttcat tcccgcctca
    13381 acaaaatccg ccagttgcag cagcatcatg cgtaaggctt tcggcatttg ctccagttca
    13441 atggcgtcca tcaggttttt cacatacagc cccagctccg tatcgccttc aatcaccagc
    13501 cgacgctgga agaagagcgt atccggatct tgtttacgcg ccgcaatcat cagcagatcg
    13561 ctggcgtcgg cactaaaact cacatcagct tgcgcgttct ggctgaccac cagtttgcca
    13621 ttcaccaccg aggtaaacca ttgcaggtca atatcacgca catgaatact taaccagcgg
    13681 ccttcaagaa actccagctc gccatcatcc agcgcctgac ggaattgcca gcttaagacc
    13741 tgctcaagaa cctggcgttt tagcgcaaat ggcgtcagtt ttaccggtac actcaacaga
    13801 gatggcccca aatgcacaat acgggaacgc agtttatcca acacgagttt tactccctgt
    13861 ttcaacaatc atcctatttt gccatatcag aaaaataaca tagcggtata aatcaacaat
    13921 tccatatgaa attgctgcta ccaccaatac aactttaact gccttaaatc aaaaattgtc
    13981 gcagcaaggt taactaaaat cccagttcgt taacattttt gcgttttgat agcgcaacct
    14041 tcaggaaaaa ttatggagct gctctgccct gccggaaatc tcccggcgct taaggcggcc
    14101 atcgaaaacg gcgcagatgc tgtttatatc gggctaaaag atgataccaa tgcccgtcac
    14161 ttcgccggcc ttaactttac cgagaaaaaa ttgcaggaag cggtgagttt tgtccatcaa
    14221 catcgccgca aacttcacat cgcgattaac acttttgcgc atccggacgg ttacgcccgt
    14281 tggcagcgtg ccgtggatat ggcggcgcag ctgggtgccg acgcgctgat cctcgccgac
    14341 ctcgccatgc tagagtacgc cgccgagcgt tacccgcata ttgagcgtca tgtgtcagtg
    14401 caggcttcgg cgaccaatga agaggcgatt aacttttatc atcgccattt tgacgttgct
    14461 cgcgtggtgc tgccgcgcgt gttgtcgatt catcaggtga aacaactggc acgggtcaca
    14521 cctgtaccac tggaagtctt tgctttcggc agcctgtgca ttatgtcgga aggtcgttgc
    14581 tatctgtcgt cgtatctgac gggtgagtcg cccaacaccg taggcgcgtg ttctccggcc
    14641 cgtttcgtgc gctggcaaca aacgccgcag gggctggaat cccgcctgaa cgaagtgctg
    14701 atcgaccgtt atcaggacgg cgaaaacgca ggttatccga cgctatgtaa agggcgttat
    14761 ctggtggacg gcgagcgcta tcacgcgctg gaagaaccaa ccagtctcaa taccctggaa
    14821 ctgctgccgg agttaatggc ggcgaatatt gcttcggtga aaattgaagg ccgccagcgt
    14881 agcccggcgt atgtcagcca ggtggcgaaa gtctggcgtc aggctatcga ccgttgtaag
    14941 gccgatccgc aaaacttcat accgcaaagc gcgtggatgg agacgctcgg gtcgatgtcc
    15001 gaaggcacgc agaccaccct tggcgcgtat caccgtaaat ggcagtgaga agagcaatga
    15061 aatattcctt agggccagtg ctgtggtact ggccaaaaga gacgctggaa gatttttatc
    15121 agcaggccgc caccagcagc gccgacgtga tttatcttgg tgaagcggta tgcagcaagc
    15181 gccgggcaac caaagttggc gactggctgg agatggcaaa atcgctcgcc ggaagtggca
    15241 agcagattgt gctctccacg ctggcgctgg tgcaggcatc ctctgaactg ggcgaactga
    15301 aacgctatgt tgagaacggt gagtttctga ttgaagccag cgatctcggc gtggtgaata
    15361 tgtgcgctga acgcaaactg ccgttcgtcg ccgggcacgc gctgaactgc tacaacgcgg
    15421 tgacgctgaa aatattgctc aaacagggca tgatgcgctg gtgtatgccg gtggagcttt
    15481 cccgcgactg gctggtgaat ctgcttaatc agtgcgatga gctgggcatt cgcaaccagt
    15541 ttgaagtgga agtcctgagc tacggtcatc taccgctggc ctactccgcc cgctgcttta
    15601 ccgcgcgttc ggaagaccgc ccgaaagatg agtgtgaaac ctgctgcatt aagtatccga
    15661 acgggcgcaa cgtgctgtcg caggaaaacc aacaagtgtt tgtactcaat ggcattcaga
    15721 ccatgagcgg ctacgtttac aacctcggta acgagctggc atccatgcag gggctggttg
    15781 atgtggtccg cctgtcaccg cagggtactg acactttcgc gatgctcgac gccttccgcg
    15841 ctaatgaaaa tggcgctgcg ccactaccgt tgacggcaaa cagcgactgt aacggctact
    15901 ggcggcggct ggccgggctg gagctgcaag cctaagtaaa tatgctcgtc atacttcaag
    15961 ttgcatgtgc tgcatctgca ttcgttcacc ccagtcactt acttatgtaa gctcctgggg
    16021 attcactctc ttgtcgcctt cctgcaactc gaattattta gagcacagct cactttgtta
    16081 acaactttaa ctactcttta atgcagtatt aaagattaat cggtaacaaa gtgagctgtt
    16141 atgactgata aaaccattgc gttttcgcta ctcgatctgg cccccattcc cgaaggttct
    16201 tcagcgcgag aagcattctc ccactctctc gatctcgcct gtctggctga aaagcgcggc
    16261 tatcatcgct actggctggc agaacaccac aatatgactg gcattgccag tgctgccacg
    16321 tcggtattga ttggctatct ggcggcgaat accaccacgc tgcatctggg gtctggcggc
    16381 gtgatgttgc ctaaccactc accgttggtc attgccgaac agttcggcac gctcaataca
    16441 ctctatccgg ggcgaatcga tttggggctg ggtcgtgcgc caggtagtga ccaacggaca
    16501 atgatggcgc tgcgtcgcca tatgagcggc gatattgata atttcccccg cgatgtcgcg
    16561 gagctggtgg actggtttga cgcccgcgat cccaatccgc atgtgcgccc ggtaccaggc
    16621 tatggcgaga aaatccccgt gtggttgtta ggctccagcc tttacagcgc gcaactggcg
    16681 gcgcagcttg gtctgccgtt cgcgtttgcc tcacacttcg cgccggatat gttgttccag
    16741 gcgctgcatc tttatcgcag caacttcaaa ccgtcggcac gactggaaaa accatacgcg
    16801 atggtgtgca tcaatattat cgccgccgat agcaaccgcg acgctgagtt tctgtttacc
    16861 tcaatgcagc aagcctttgt gaagttgcgc cgtggcgaaa ccgggcaact gccgccaccg
    16921 attcaaaata tggatcagtt ctggtcgccg tccgaacagt atggtgtgca gcaggcgctg
    16981 agtatgtcgt tggtaggtga taaagcgaaa gtgcgtcatg gcttgcagtc gatcctgcgc
    17041 gaaaccgacg ccgatgagat tatggtcaac gggcagattt tcgaccacca ggcgcggctg
    17101 cattcgtttg agctggcgat ggatgttaag gaagagttgt tgggatagtg tgtcttaacg
    17161 cgggaagcct tatccgagct ggcaacgctg tcc
//
LOCUS       DAZKHF020000081        16765 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2201S31241
            SAMN43267800-rid25106413.denovo.081, whole genome shotgun sequence.
ACCESSION   DAZKHF020000081 DAZKHF020000000
VERSION     DAZKHF020000081.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267800
            Sequence Read Archive: SRR30307263
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 16765)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16765)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 16765)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350581.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350581.2
            Genome Coverage       :: 151x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:21:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,652
            CDSs (total)                      :: 5,557
            Genes (coding)                    :: 5,320
            CDSs (with protein)               :: 5,320
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 237
            CDSs (without protein)            :: 237
            Pseudo Genes (ambiguous residues) :: 0 of 237
            Pseudo Genes (frameshifted)       :: 102 of 237
            Pseudo Genes (incomplete)         :: 130 of 237
            Pseudo Genes (internal stop)      :: 52 of 237
            Pseudo Genes (multiple problems)  :: 41 of 237
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16765
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267800-rid25106413.denovo.081"
                     /serotype="O 26:H11"
                     /isolate="2201S31241"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-01"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            complement(130..612)
                     /gene="ybiA"
                     /locus_tag="ACHOSC_RS11165"
                     /old_locus_tag="pgaptmp_002233"
     CDS             complement(130..612)
                     /gene="ybiA"
                     /locus_tag="ACHOSC_RS11165"
                     /old_locus_tag="pgaptmp_002233"
                     /EC_number="3.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-glycosidase YbiA"
                     /protein_id="HGU4548163.1"
                     /translation="MPVRAQRIQHVMQDTIINFYSTSDDYGDFSNFAAWPIKVDGKTW
                     PTSEHYFQAQKFLDEKYREEIRRVSSPMVAARMGRDRSKPLRKNWESVKEQVMRKALR
                     AKFEQHPELRALLLATAPAKLVEHTENDAYWGDGGNGKGKNRLGYLLMELREQLAIEK
                     "
     gene            732..2882
                     /gene="dinG"
                     /locus_tag="ACHOSC_RS11170"
                     /old_locus_tag="pgaptmp_002234"
     CDS             732..2882
                     /gene="dinG"
                     /locus_tag="ACHOSC_RS11170"
                     /old_locus_tag="pgaptmp_002234"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016818 - hydrolase activity, acting on
                     acid anhydrides, in phosphorus-containing anhydrides
                     [Evidence IEA];
                     GO_process: GO:0006139 - nucleobase-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase DinG"
                     /protein_id="HGU4548164.1"
                     /translation="MALTAALKAQIAAWYKALQEQIPDFIPRAPQRQMIADVAKTLAG
                     EEGRHLAIEAPTGVGKTLSYLIPGIAIAREEQKTLVVSTANVALQDQIYSKDLPLLKK
                     IIPDLKFTAAFGRGRYVCPRNLTALASTEPTQQDLLAFLDDELTPNNQEEQKRCAKLK
                     GDLDTYKWDGLRDHTDIAIDDDLWRRLSTDKASCLNRNCYYYRECPFFVARREIQEAE
                     VVVANHALVMAAMESEAVLPDPKNLLLVLDEGHHLPDVARDALEMSAEITAPWYRLQL
                     DLFTKLVATCMEQFRPKTIPPLAIPERLNAHCEELYELIASLNNILNLYMPAGQEAEH
                     RFAMGELPDEVLEICQRLAKLTEMLRGLAELFLNDLSEKTGSHDIVRLHRLILQMNRA
                     LGMFEAQSKLWRLASLAQSSGAPVTKWATREEREGQLHLWFHCVGIRVSDQLERLLWR
                     SIPHIIVTSATLRSLNSFSRLQEMSGLKEKAGDRFVALDSPFNHCEQGKIVIPRMRFE
                     PSIDNEEQHIAEMAAFFRKQVESKKHLGMLVLFASGRAMQRFLDYVTDLRLMLLVQGD
                     QPRYRLVELHRKRVANGERSVLVGLQSFAEGLDLKGDLLSQVHIHKIAFPPIDSPVVI
                     TEGEWLKSLNRYPFEVQSLPSASFNLIQQVGRLIRSHGCWGEVVIYDKRLLTKNYGKR
                     LLDALPVFPIEQPEVPEGIVKKKEKTKSPRRRRR"
     gene            2910..3872
                     /gene="ybiB"
                     /locus_tag="ACHOSC_RS11175"
                     /old_locus_tag="pgaptmp_002235"
     CDS             2910..3872
                     /gene="ybiB"
                     /locus_tag="ACHOSC_RS11175"
                     /old_locus_tag="pgaptmp_002235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein YbiB"
                     /protein_id="HGU4548165.1"
                     /translation="MDYRKIIKEIGRGKNHARDLDRDTARGLYAHMLNGEVPDLELGG
                     VLIALRIKGEGEAEMLGFYEAMQNHTIKLTPPAGKPMPIVIPSYNGARKQANLTPLLA
                     ILLHKLGFPVVVHGVSEDPTRVLTETIFELMGITPTLHGGQAQAKLDEHQPVFMPVGA
                     FCPPLEKQLAMRWRMGVRNSAHTLAKLATPFAEGEALRLSSVSHPEYIGRVAKFFSDI
                     GGRALLMHGTEGEVYANPQRCPQINLIDREGMRVLYEKQDTAGSELLPQAKDPETTAQ
                     WIERCLAGSEPIPESLKIQMACCLVATGEAATISDGLARVNQAF"
     gene            4013..5098
                     /gene="hcxB"
                     /locus_tag="ACHOSC_RS11180"
                     /old_locus_tag="pgaptmp_002236"
     CDS             4013..5098
                     /gene="hcxB"
                     /locus_tag="ACHOSC_RS11180"
                     /old_locus_tag="pgaptmp_002236"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxycarboxylate dehydrogenase HcXB"
                     /protein_id="HGU4548166.1"
                     /translation="MESGHRFDAQTLHSFIQAVFRQMGSEEQEAKLVADHLIAANLAG
                     HDSHGIGMIPSYVRSWSQGHLQINHHAKTVKESGAAVTLDGDRAFGQVAAHEAMALGI
                     EKAHKHGIAAVALHNSHHIGRIGYWAEQCAAAGFVSIHFVSVVGIPMVAPFHGRDSRF
                     GTNPFCVVFPRKDNFPLLLDYATSAIAFGKTRVAWHKGVPVPPGCLIDVNGVPTTNPA
                     VMQESPLGALLTFAEHKGYALAAMCEILGGALSGGKTTHQETLQTSPDAILNCMTTII
                     INPELFGAPDCNAQTEAFAEWVKASPHDDDKPILLPGEWEVNTRRERQKQGIPLDAGS
                     WQAICDAARQIGMPEETLQAFCQQLAS"
     gene            5165..5224
                     /gene="ybiE"
                     /locus_tag="ACHOSC_RS11185"
                     /old_locus_tag="pgaptmp_002237"
     CDS             5165..5224
                     /gene="ybiE"
                     /locus_tag="ACHOSC_RS11185"
                     /old_locus_tag="pgaptmp_002237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YbiE"
                     /protein_id="HGU4548167.1"
                     /translation="MRLSANRQGNTCAGRSVNA"
     gene            complement(5329..6693)
                     /gene="rhlE"
                     /locus_tag="ACHOSC_RS11190"
                     /old_locus_tag="pgaptmp_002238"
     CDS             complement(5329..6693)
                     /gene="rhlE"
                     /locus_tag="ACHOSC_RS11190"
                     /old_locus_tag="pgaptmp_002238"
                     /EC_number="3.6.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003724 - RNA helicase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RNA helicase RhlE"
                     /protein_id="HGU4548168.1"
                     /translation="MSFDSLGLSPDILRAVAEQGYREPTPIQQQAIPAVLEGRDLMAS
                     AQTGTGKTAGFTLPLLQHLITRQPHAKGRRPVRALILTPTRELAAQIGENVRDYSKYL
                     NIRSLVVFGGVSINPQMMKLRGGVDVLVATPGRLLDLEHQNAVKLDQVEILVLDEADR
                     MLDMGFIHDIRRVLTKLPAKRQNLLFSATFSDDIKALAEKLLHNPLEIEVARRNTASD
                     QVTQHVHFVDKKRKRELLSHMIGKGNWQQVLVFTRTKHGANHLAEQLNKDGIRSAAIH
                     GNKSQGARTRALADFKSGDIRVLVATDIAARGLDIEELPHVVNYELPNVPEDYVHRIG
                     RTGRAAATGEALSLVCVDEHKLLRDIEKLLKKEIPRIAIPGYEPDPSIKAEPIQNGRQ
                     QRGDGGRGQGGGRGQQQPRRGEGGAKSASAKPAEKPSRRLGDAKPAGEQQRRRRPRKP
                     TAAQ"
     gene            6922..7593
                     /gene="cecR"
                     /locus_tag="ACHOSC_RS11195"
                     /old_locus_tag="pgaptmp_002239"
     CDS             6922..7593
                     /gene="cecR"
                     /locus_tag="ACHOSC_RS11195"
                     /old_locus_tag="pgaptmp_002239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415317.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator CecR"
                     /protein_id="HGU4548169.1"
                     /translation="MNNPAMTIKGEQAKKQLIAAALAQFGEYGMNATTREIAAQAGQN
                     IAAITYYFGSKEDLYLACAQWIADFIGEQFRPHAEEAERLFAQPQPDRAAIRELILRA
                     CRNMIKLLTQDDTVNLSKFISREQLSPTAAYHLVHEQVISPLHSHLTRLIAAWTGCDA
                     NDTRMILHTHALIGEILAFRLGKETILLRTGWTAFDEEKTELINQTVTCHIDLILQGL
                     SQRSL"
     gene            7596..8591
                     /gene="hlyD"
                     /locus_tag="ACHOSC_RS11200"
                     /old_locus_tag="pgaptmp_002240"
     CDS             7596..8591
                     /gene="hlyD"
                     /locus_tag="ACHOSC_RS11200"
                     /old_locus_tag="pgaptmp_002240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308900.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="secretion protein HlyD"
                     /protein_id="HGU4548170.1"
                     /translation="MKKPVVIGLAVVVLAAVVAGGYWWYQSRQDNGLTLYGNVDIRTV
                     NLSFRVGGRVESLAVDEGDAIKAGQVLGELDHKPYEIALMQAKAGVSVAQAQYDLMLA
                     GYRDEEIAQAAAAVKQAQAAYDYAQNFYNRQQGLWKSRTISANDLENARSSRDQAQAT
                     LKSAQDKLRQYRSGNREQDIAQAKASLEQAQAQLAQAELNLQDSTLIAPSDGTLLTRA
                     VEPGTVLNEGGTVFTVSLTRPVWVRAYVDERNLDQAQPGRKVLLYTDGRPDKPYHGQI
                     GFVSPTAEFTPKTVETPDLRTDLVYRLRIVVTDADDALRQGMPVTVQFGNEAGHE"
     gene            8584..10320
                     /gene="ybhF"
                     /locus_tag="ACHOSC_RS11205"
                     /old_locus_tag="pgaptmp_002241"
     CDS             8584..10320
                     /gene="ybhF"
                     /locus_tag="ACHOSC_RS11205"
                     /old_locus_tag="pgaptmp_002241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308899.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HGU4548171.1"
                     /translation="MNDAVITLNGLEKRFPGMDKPAVAPLDCTIHAGYVTGLVGPDGA
                     GKTTLMRMLAGLLKPDSGNATVIGFDPIKNDGALHAVLGYMPQKFGLYEDLTVMENLN
                     LYADLRSVTGEARKQTFARLLEFTSLGPFTGRLAGKLSGGMKQKLGLACTLVGEPKVL
                     LLDEPGVGVDPISRRELWQMVHELAGEGMLILWSTSYLDEAEQCRDVLLMNEGELLYQ
                     GEPKALTQTMAGRSFLMTSPHEGNRKLLQRALKLPQVSDGMIQGKSVRLILKKEATPD
                     DIRHADGMPEININETTPRFEDAFIDLLGGAGTSESPLGAILHTVEGTPGETVIEAKE
                     LTKKFGDFAATDHVNFAVKRGEIFGLLGPNGAGKSTTFKMMCGLLVPTSGQALVLGMD
                     LKESSGKARQHLGYMAQKFSLYGNLTVEQNLRFFSGVYGLRGRAQNEKISRMSEAFGL
                     KSIASHATDELPLGFKQRLALACSLMHEPDILFLDEPTSGVDPITRREFWLHINSMVE
                     KGVTVMVTTHFMDEAEYCDRIGLVYRGKLIASGTPDDLKAQSANDEQPDPTMEQAFIQ
                     LIHDWDKEHSNE"
     gene            10313..11446
                     /gene="ybhS"
                     /locus_tag="ACHOSC_RS11210"
                     /old_locus_tag="pgaptmp_002242"
     CDS             10313..11446
                     /gene="ybhS"
                     /locus_tag="ACHOSC_RS11210"
                     /old_locus_tag="pgaptmp_002242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HGU4548172.1"
                     /translation="MSNPILSWRRVRALCVKETRQIVRDPSSWLIAVVIPLLLLFIFG
                     YGINLDSSKLRVGILLEQRSEAALDFTHTMTGSPYIDATISDNRQELIAKMQAGKIRG
                     LVVIPVDFAEQMERANATAPIQVITDGSEPNTANFVQGYVEGIWQIWQMQRAEDNGQT
                     FEPLIDVQTRYWFNPAAISQHFIIPGAVTIIMTVIGAILTSLVVAREWERGTMEALLS
                     TEITRTELLLCKLIPYYFLGMLAMLLCMLVSVFILGVPYRGSLLILFFISSLFLLSTL
                     GMGLLISTITRNQFNAAQVALNAAFLPSIMLSGFIFQIDSMPAVIRAVTYIIPARYFV
                     STLQSLFLAGNIPVVLVVNVLFLIASAVMFIGLTWLKTKRRLD"
     gene            11457..12563
                     /gene="ybhR"
                     /locus_tag="ACHOSC_RS11215"
                     /old_locus_tag="pgaptmp_002243"
     CDS             11457..12563
                     /gene="ybhR"
                     /locus_tag="ACHOSC_RS11215"
                     /old_locus_tag="pgaptmp_002243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HGU4548173.1"
                     /translation="MFHRLWTLIRKELQSLLREPQTRAILILPVLIQVILFPFAATLE
                     VTNATIAIYDEDNGEHSVELTQRFARASAFTHVLLLKSPQEIRPTIDTQKALLLVRFP
                     ADFSRKLDTFQTAPLQLILDGRNSNSAQIAANYLQQIVKNYQQELLEGKPKPNNSELV
                     VRNWYNPNLDYKWFVVPSLIAMITTIGVMIVTSLSVAREREQGTLDQLLVSPLTTWQI
                     FIGKAVPALIVATFQATIVLAIGIWAYQIPFAGSLALFYFTMVIYGLSLVGFGLLISS
                     LCSTQQQAFIGVFVFMMPAILLSGYVSPVENMPVWLQNLTWINPIRHFTDITKQIYLK
                     DASLDIVWNSLWPLLVITATTGSAAYAMFRRKVM"
     gene            complement(12525..12935)
                     /gene="ybhQ"
                     /locus_tag="ACHOSC_RS11220"
                     /old_locus_tag="pgaptmp_002244"
     CDS             complement(12525..12935)
                     /gene="ybhQ"
                     /locus_tag="ACHOSC_RS11220"
                     /old_locus_tag="pgaptmp_002244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbhQ family protein"
                     /protein_id="HGU4548174.1"
                     /translation="MKWQQRVRVATGLSCWQIMLHLLVVALLVVGWMSKTLVHVGVGL
                     CALYCVTVVMMLVFQRHPEQRWREVADVLEELTTTWYFGAALIVLWLLSRVLENNFLL
                     AIAGLAILAGPAVVSLLAKDKKLHHLTSKHRVRR"
     gene            13068..13829
                     /gene="ybhP"
                     /locus_tag="ACHOSC_RS11225"
                     /old_locus_tag="pgaptmp_002245"
     CDS             13068..13829
                     /gene="ybhP"
                     /locus_tag="ACHOSC_RS11225"
                     /old_locus_tag="pgaptmp_002245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HGU4548175.1"
                     /translation="MPDQTQQFSFKVLTINIHKGFTAFNRRFILPELRDAVRTVSADI
                     VCLQEVMGAHEVHPLHVENWPDTSHYEFLADTMWSDFAYGRNAVYPKGHHGNAVLSRY
                     PIEHYENRDVSVDGAEKRGVLYCRIVPPMTGKAIHVMCVHLGLREAHRQAQLAMLAEW
                     VNELPDGEPVLVAGDFNDWRQKANHPLKVQAGLDEIFTRAHGRPARTFPVQFPLLRLD
                     RIYVKNASASAPTALPLRTWRHLSDHAPLSAEIHL"
     gene            13826..15067
                     /gene="clsB"
                     /locus_tag="ACHOSC_RS11230"
                     /old_locus_tag="pgaptmp_002246"
     CDS             13826..15067
                     /gene="clsB"
                     /locus_tag="ACHOSC_RS11230"
                     /old_locus_tag="pgaptmp_002246"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008808 - cardiolipin synthase activity
                     [Evidence IEA];
                     GO_process: GO:0032049 - cardiolipin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cardiolipin synthase ClsB"
                     /protein_id="HGU4548176.1"
                     /translation="MKCSWREGNKIQLLENGEQYYPAVFKAIGEAQERIILETFIWFE
                     DDVGKQLHAALLAAAQRGVKAEVLLDGYGSPDLSDEFVNELTAAGVVFRYYDPRPRLF
                     GMRTNVFRRMHRKIVVIDARIAFIGGLNYSAEHMSSYGPEAKQDYAVRLEGPIVEDIL
                     QFELENLPGQSAARRWWRRHHKAEENRQPGEAQVLLVWRDNEEHRDDIERHYLKMLTQ
                     ARREVIIANAYFFPGYRFLHALRKAARRGVRIKLIIQGEPDMPIVRVGARLLYNYLVK
                     GGVQVFEYRRRPLHGKVALMDDHWATVGSSNLDPLSLSLNLEANVIIHDRHFNQTLRD
                     NLNGIIAADCQQVDETMLPKRTWWNLTKSVLAFHFLRHFPALVGWLPAHTPRLAQVDP
                     PAQPTMETQDRVETENTGVKP"
     gene            15067..16023
                     /gene="ybhN"
                     /locus_tag="ACHOSC_RS11235"
                     /old_locus_tag="pgaptmp_002247"
     CDS             15067..16023
                     /gene="ybhN"
                     /locus_tag="ACHOSC_RS11235"
                     /old_locus_tag="pgaptmp_002247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysylphosphatidylglycerol synthase
                     domain-containing protein"
                     /protein_id="HGU4548177.1"
                     /translation="MSKSHPRWRLAKKILTWLFFIAVIVLLVVYAKKVDWEEVWKVIR
                     DYNRVALLSAVGLVVVSYLIYGCYDLLARFYCGHKLAKRQVMLVSFICYAFNLTLSTW
                     VGGIGMRYRLYSRLGLPGSTITRIFSLSITTNWLGYILLAGIIFTAGVVELPDHWYVD
                     QTTLRILGIGLLMIIAVYLWFCAFAKHRHMTIKGQKLVLPSWKFALAQMLISSVNWMV
                     MGAIIWLLLGQSVNYFFVLGVLLVSSIAGVIVHIPVGIGVLEAVFIALLAGEHTSKGS
                     IIAALLAYRVLYYFIPLLLALICYLLLESQAKKLRAKNEAAM"
     gene            complement(16059..>16765)
                     /gene="ybhM"
                     /locus_tag="ACHOSC_RS11240"
                     /old_locus_tag="pgaptmp_002248"
     CDS             complement(16059..>16765)
                     /gene="ybhM"
                     /locus_tag="ACHOSC_RS11240"
                     /old_locus_tag="pgaptmp_002248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706665.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="Bax inhibitor-1/YccA family protein"
                     /protein_id="HGU4548178.1"
                     /translation="YSQNSNKLDFQHETRILNGIWLITALGLVATAGLAWGAKYLEIT
                     ATKYDSPTMYVAIGLLLVCMYGLSKDINKINAVIAGVIYLFLLSLVAIVVASLAPVPA
                     IIVVFSTAGAMFLISMLAGLLFNVDPGSHRFIIMMTLTGLALVIIMNAALMSERPVWV
                     ISCLMIVLWSGIISHGRNKLLELAGKCHSEELWSPVRCAVTGALTLYYYFIGFFGILA
                     AIAITLVWQRHTRFFH"
BASE COUNT         3897 a         4377 c         4606 g         3885 t
ORIGIN      
        1 atttgcatgt ttttgtaggc cggataaggc gtttacgccg catccggcat ttcacaacaa
       61 gcacttgtca gcaatttgag aacacgggaa aaaatttatc tgttttaccg cccataagcc
      121 accgagacgt tacttctcta tagccaattg ttcgcgcaac tccattaaaa ggtagcccag
      181 tcgattcttg cccttaccat taccaccgtc tccccagtaa gcatcgtttt ccgtatgctc
      241 aaccagtttt gcgggcgcgg ttgccagcaa gagcgcacgc agttctggat gctgttcgaa
      301 tttagcgcga agtgcttttc gcatcacctg ttctttgacc gactcccagt ttttacgcag
      361 aggcttagaa cgatcgcgcc ccatgcgtgc ggcgaccata ggggaagaaa cccgacggat
      421 ctcttcgcgg tatttttcat caaggaattt ttgtgcctga aaatagtgtt ctgaggtggg
      481 ccaggttttc ccgtcaacct tgatgggcca ggcggcaaag ttagaaaaat caccgtagtc
      541 atcgctggtg ctgtagaaat ttatgatggt gtcttgcatg acgtgttgga tcctttgtgc
      601 tcgaacgggc attaaaccgc attatgttgg tggttattgc gagccgcttt ccagaaacag
      661 aaaaaccatt acccctgaaa accgaaaaat gccacaatat tggctgttta tacagtattt
      721 caggttttct catggcatta accgccgcgc ttaaagcgca aattgccgcc tggtataagg
      781 cgcttcagga acagatcccc gactttattc cccgtgcgcc gcagcggcag atgattgcgg
      841 acgtcgccaa aacgctggcc ggagaagaag ggcgacatct ggcgattgaa gcccccaccg
      901 gcgttgggaa aacgctctcc tatttgatcc ccggcatcgc cattgcccgc gaagagcaaa
      961 aaacgctggt ggtgagtacc gccaacgtgg cattgcagga tcagatttac agcaaagatt
     1021 taccgctgct gaaaaagatc attcccgatc ttaaattcac tgccgctttt gggcgtgggc
     1081 gctacgtttg cccgcgtaat ctgacggcgc tcgccagcac tgagcccacg caacaggatc
     1141 tgctggcgtt tcttgacgac gaactgacgc caaataatca ggaagagcaa aaacgttgtg
     1201 cgaagctgaa gggcgatctc gacacttata aatgggatgg tctgcgcgat catactgata
     1261 tcgctataga tgacgatctc tggcgtcgtt taagtaccga caaagccagc tgcctcaacc
     1321 gcaactgtta ctactatcgt gaatgcccgt tttttgttgc tcgtcgggag attcaggaag
     1381 cggaagtggt ggtggcaaac catgcgctgg tgatggcggc gatggaaagc gaagccgtac
     1441 tacctgaccc gaaaaatctg ctgctggtgc tggacgaagg tcatcacctg ccagatgtgg
     1501 cccgggatgc gctggagatg agtgccgaaa tcaccgcgcc ctggtatcga ctacagctgg
     1561 acttgttcac gaaactggtc gctacctgca tggagcagtt tcgcccgaag accatcccac
     1621 cgctggcgat ccctgaacgt ttgaatgcgc attgtgaaga gttgtatgag cttatcgcct
     1681 cgttaaacaa cattctcaat ctctacatgc ctgccgggca ggaggcagag caccgttttg
     1741 cgatgggcga actgcctgat gaagtgctgg agatctgcca gcggctggca aaactcaccg
     1801 agatgctgcg tggtctggcg gagttatttc ttaacgattt aagtgagaaa accggcagcc
     1861 acgatattgt gcgtctgcat cggttgattt tgcagatgaa ccgcgcgttg gggatgttcg
     1921 aggcgcaaag caaactctgg cggctggcgt ctctggcgca atcttccggt gcgccggtga
     1981 ccaaatgggc gacgcgggaa gagcgcgaag ggcagctgca tctctggttt cactgcgtgg
     2041 gaatacgtgt cagcgatcag ctggaaaggc tgctgtggcg cagtattccg cacattattg
     2101 tcacctccgc aaccttgcgt tcgctgaaca gtttttcgcg tttgcaggag atgagtggtc
     2161 tgaaagagaa agcgggcgac cgttttgtgg cgctggattc cccctttaac cactgcgaac
     2221 agggcaaaat tgttattccc cggatgcgct ttgagccttc catcgacaac gaagagcagc
     2281 atattgccga aatggcggcc tttttccgta agcaggtgga gagcaaaaaa catctcggta
     2341 tgttggtact gtttgccagc ggacgggcga tgcagcgctt tctcgactat gtgacggatt
     2401 tacgcctgat gctgctggtg cagggcgatc agccgcgtta tcgcttagtt gaactgcacc
     2461 gcaaacgcgt tgccaacggt gagcgtagtg tgctggtggg cttacagtca tttgccgaag
     2521 ggcttgattt gaaaggtgat ctgctcagcc aggtgcatat ccacaaaatc gcttttccgc
     2581 ccatcgacag cccggtggtg atcaccgaag gggaatggct gaaaagcctc aaccgctatc
     2641 cgtttgaggt gcaaagcctg ccgagcgcct cgtttaacct gattcagcag gttgggcgac
     2701 tgattcgaag ccacggttgc tggggcgaag tggttatcta cgacaaacgc ttgctgacca
     2761 aaaattatgg caagcgacta ctggatgcat taccggtatt tccgatagag caaccggaag
     2821 tccctgaagg tatagttaaa aagaaagaaa aaacgaaatc cccacgccgt cggcggcgtt
     2881 aatgatgtga gtcaggtaag gagtcgtaaa tggactatcg caaaatcatt aaagagatcg
     2941 ggcgcgggaa aaaccacgcg cgcgatttag accgggatac tgcgcgcggt ctgtatgccc
     3001 atatgctcaa tggtgaagtc cctgaccttg agttgggcgg cgtattgatt gcgctgcgta
     3061 tcaaagggga aggggaagca gagatgctcg gcttttacga agccatgcaa aatcacacca
     3121 tcaagctgac gccgcctgca ggcaagccga tgccgattgt catccccagt tacaacggcg
     3181 cgcgtaaaca ggccaacctg acgccgttgt tggcgattct cctgcacaaa ctcggttttc
     3241 ctgtggtggt tcacggggtt agcgaagatc caacccgcgt gctgactgaa accatttttg
     3301 aattgatggg tattacgcca acgttgcacg gcggacaggc gcaggcgaag ctcgacgaac
     3361 atcaaccggt gtttatgcca gtcggcgcgt tttgcccgcc gctggaaaaa caactggcga
     3421 tgcgctggcg gatgggcgtg cgtaacagtg cacataccct ggcgaaactg gcaacgccat
     3481 ttgccgaagg tgaggcgctg cgtctttcca gcgtttcgca tccggaatac attggacgcg
     3541 tcgcgaagtt ctttagcgat atcggcgggc gggcgctact aatgcatggt actgaaggtg
     3601 aagtgtatgc taatccgcag cgctgcccgc agatcaatct cattgaccgt gaagggatgc
     3661 gggtgctgta tgaaaaacag gacactgctg gtagcgagtt actgccacaa gcaaaagatc
     3721 cggaaaccac ggcgcagtgg attgagcgtt gccttgctgg cagcgaaccg attcccgaat
     3781 cgctgaaaat ccagatggct tgctgcctgg tggctacggg tgaagcggca actatcagcg
     3841 acggcctggc gcgcgttaat caggcatttt aattcttttc tcagccggat gagccatgtc
     3901 atccggcttt taccccaccg caatatgaaa ttcctgcatc tttattgacc ttcccacgcc
     3961 cggcgtgcag cataaaaata caacaaacac ataacataaa caggagttaa ccatggaaag
     4021 tggtcatcgc tttgatgctc agacgctgca cagttttatt caggctgtat ttcgtcagat
     4081 gggtagcgag gaacaagaag cgaaattagt tgccgatcat ttaatcgcgg caaacctggc
     4141 agggcatgat tcacatggta ttggcatgat cccaagctat gtacgctcct ggagtcaggg
     4201 gcacctgcaa attaaccatc atgccaaaac cgttaaagag tcgggggcgg cggtcacgct
     4261 cgatggcgat cgcgcatttg gtcaggtcgc ggcacatgaa gcgatggcgc tggggattga
     4321 gaaagcgcat aagcacggta ttgccgccgt ggcgctacat aactcgcatc atatcggccg
     4381 tatcggttac tgggcggagc agtgtgcagc ggcggggttt gtctctatcc actttgttag
     4441 cgtggtcggt attccaatgg tcgcgccgtt ccacggtcgc gacagccgct ttggcaccaa
     4501 tccgttctgt gtggttttcc ctcgtaaaga taatttcccg ctgttgcttg attacgccac
     4561 cagcgccatt gcatttggta aaactcgcgt cgcctggcat aaaggcgtcc ccgtgccgcc
     4621 aggttgcctg attgacgtta acggcgtgcc gacgaccaat ccggcggtaa tgcaggagtc
     4681 gccgttgggg gcgctgttga cctttgccga acataaaggc tacgcccttg cagcgatgtg
     4741 tgaaattctt ggcggggcgc tttctggcgg taaaacgacg catcaggaaa cgttacaaac
     4801 cagtcccgat gccattctta actgcatgac cactatcatc atcaacccgg aactcttcgg
     4861 cgcgccggat tgtaacgcgc agaccgaagc ctttgccgag tgggtgaaag cctcgccgca
     4921 tgatgatgat aagccgattt tgctaccggg cgagtgggaa gtgaacacgc gtcgcgaacg
     4981 gcagaagcag gggattccac tggatgcggg aagctggcag gccatttgtg atgcagcgcg
     5041 gcagattggt atgccggaag agacgttgca ggctttctgt cagcagttag ccagctaaaa
     5101 aaaagcccgt ccagtggcgg acgggcaaac aagggtaaca taggatcaat gagggttaga
     5161 gcatatgcgt ctgtcggcaa acagacaggg aaatacttgt gctggacgta gcgtaaacgc
     5221 ctgatccgtc caacggttcg gattttgtag atactgataa gcgtagcgca tcaggcagtt
     5281 ttgcgtttgt tcatcagcct gatgccgggc atagcccggc ataaaagatt actgcgcagc
     5341 ggtaggttta cgcggacggc ggcgacgttg ttgttcgcct gccggtttgg catcgccgag
     5401 gcggcgagac ggtttttctg caggtttcgc gcttgcagat tttgcgccac cttccccacg
     5461 gcgtggttgc tgttgaccgc gaccaccacc ttgcccacga ccgccgtcgc cacgttgctg
     5521 gcgaccgttc tggatcggtt cggctttgat tgacgggtcc ggctcatagc ccggaatcgc
     5581 aatgcgcggg atctcttttt tcagcagttt ttcgatatca cgcagcagtt tgtgttcatc
     5641 aacacacacc agcgacaacg cttcaccggt agcagccgca cgaccggtac gcccgatacg
     5701 gtggacataa tcttccggta cgtttggcag ttcatagttg acgacgtgcg gcaactcttc
     5761 gatatccaga ccgcgcgcag cgatatcggt cgccaccagt acgcgaatgt cacccgattt
     5821 aaaatcagcc agcgcacggg tacgcgcacc ttgcgattta ttcccgtgga tcgccgcact
     5881 acggatgcca tctttattga gctgctcagc cagatggtta gcgccgtgtt tggtacgggt
     5941 aaacaccagc acctgctgcc agttcccttt accaatcatg tgcgacagca attcgcgttt
     6001 gcgtttctta tcaacaaagt gaacgtgctg ggtcacctga tcagacgcgg tattgcggcg
     6061 tgccacttcg atttccagcg ggttgtgcaa cagtttttcc gccagggctt taatatcgtc
     6121 agagaaggtc gcagagaaca gcaggttctg gcgcttcgct ggcagttttg ttaacacgcg
     6181 acggatatcg tggataaagc ccatgtcgag catgcggtca gcttcatcga ggacgaggat
     6241 ttcaacctga tccagcttca ctgcattttg atgttccagg tccagcaaac gtcccggtgt
     6301 tgccaccaga acatcaacgc cgccgcgcag cttcatcatt tgcgggttaa tgctgacacc
     6361 gccaaacacc accagcgaac gaatgttcaa atatttgctg taatcacgga cgttttcgcc
     6421 aatctgcgca gccagttcac gggtcggggt aagaatgagc gcacgtaccg gacgacgccc
     6481 tttggcgtgc ggctggcgag tgatcaggtg ttgcaacagc ggcagcgtaa agcccgctgt
     6541 tttaccggta ccggtctggg cgctagccat caggtcgcgg ccttccagca ccgcagggat
     6601 cgcctgctgc tgaatagggg tgggttcacg gtaaccctgc tcggcaacgg cgcgcaggat
     6661 atcagggctt aaacccaaag aatcgaaaga cataactact ccgaaccgcc ccgaccgtta
     6721 ccggtgtagt tttcagggag atactgaaaa ggaaaatgac aaaaaccaca atgtcatgag
     6781 ggagcggagt gtagcagttt ttgtgacgca gcgcataaat tatccctgtg actggcgtag
     6841 ctaaaattct tagccatact ggacaactcc catatggagt cataatctta atcaatcatt
     6901 tgattaagaa tggactgtgc gatgaataat cctgccatga caatcaaggg tgaacaggcg
     6961 aaaaaacagc tgattgctgc cgcactggcg cagtttggtg aatatggaat gaacgccacc
     7021 acccgcgaga tagccgccca ggccgggcag aatatcgctg ccatcaccta ctacttcggt
     7081 tcgaaagaag atttgtacct cgcctgcgcc cagtggattg ccgattttat tggcgagcag
     7141 ttccgtccgc acgccgaaga ggccgaacgc ttgttcgcac aaccacagcc tgatcgcgca
     7201 gccatccgtg aactgatcct tcgcgcctgc aggaacatga ttaagctgct cacccaggat
     7261 gacaccgtca acctcagcaa gtttatctcc cgtgagcagc tctctcccac ggcagcctat
     7321 cacctggtgc acgaacaggt gattagcccg ctacacagcc acctgacacg cctgattgcc
     7381 gcctggaccg gctgcgacgc caacgatacc cgcatgatcc tccatactca tgcgttgatt
     7441 ggtgagattc tggcgttccg tcttggcaaa gaaacaattc tgttacgtac cggctggacc
     7501 gcgttcgatg aagaaaagac cgaactgatc aaccagacgg tgacctgtca tatcgacctg
     7561 attttgcaag gattatcgca aaggagtttg tagtgatgaa aaaacctgtc gtgatcggac
     7621 tggcggtagt ggtacttgcc gccgtggttg ccggaggcta ctggtggtat caaagccgcc
     7681 aggataacgg cctgacgctg tatggcaacg tggatattcg tacggtgaat cttagtttcc
     7741 gtgttggggg gcgcgttgaa tcgctggcgg tggacgaagg tgatgctatc aaagcgggcc
     7801 aggtgctggg cgaactggat cacaagccgt atgagattgc cctgatgcag gcgaaagcgg
     7861 gtgtttcggt ggcacaggcg cagtatgacc tgatgcttgc cgggtatcgc gatgaagaaa
     7921 tcgctcaggc cgccgcagcg gtgaaacagg cgcaagccgc ctatgactat gcgcagaact
     7981 tctataaccg ccagcaaggg ttgtggaaaa gccgcaccat ttcggcaaac gacctggaaa
     8041 atgcccgctc ctcgcgcgac caggcgcagg caacgctgaa atcagcacag gataaattgc
     8101 gtcagtaccg ttccggtaac cgtgaacagg acatcgccca ggcgaaagcc agcctcgaac
     8161 aggcgcaggc gcaactggcg caggcggagt tgaatttgca ggactcaacg ttgatagccc
     8221 cgtctgatgg cacgctgtta acgcgcgcgg tggagccagg cacggtcctc aatgaaggtg
     8281 gcacggtgtt taccgtttca ctaacgcgtc cggtgtgggt gcgcgcttat gttgatgaac
     8341 ggaatcttga ccaggcccag ccggggcgca aagtgctgct ttataccgat ggtcgcccgg
     8401 acaagccgta tcacgggcag attggtttcg tttcgccgac tgctgaattt accccgaaaa
     8461 ccgtcgaaac accggatctg cgtaccgacc tcgtctatcg cctgcgtatt gtggtgaccg
     8521 acgccgatga tgcgttacgc cagggaatgc cggtgacggt gcaattcggt aacgaggcag
     8581 gacatgaatg atgccgttat cacgctgaac ggcctggaaa aacgctttcc gggcatggac
     8641 aagcccgccg tcgcgccgct cgattgtacc attcacgccg gttatgtgac ggggctggtg
     8701 gggccggatg gtgcaggtaa aaccacgcta atgcggatgc tggcgggatt actgaaaccc
     8761 gacagcggca atgccacggt gattggcttt gatccaatca aaaacgacgg cgcgctgcac
     8821 gccgtgctcg gctatatgcc gcagaaattt ggtctgtatg aagatctcac ggtgatggag
     8881 aacctcaatc tgtacgcgga tttgcgcagc gtcaccggcg aggcacgtaa gcaaactttt
     8941 gctcgcctgc tggagtttac gtctcttggg ccgtttaccg gacgcctggc gggcaagctc
     9001 tccggtggga tgaaacaaaa actcggtctg gcctgtaccc tggtgggcga accgaaagtg
     9061 ttgctgctcg atgaacccgg cgtcggcgtt gaccctatct cacggcgcga actgtggcag
     9121 atggtgcatg agctggcagg cgaagggatg ttaatcctct ggagtacctc gtatctcgac
     9181 gaagccgagc agtgccgtga cgtgttactg atgaacgaag gcgagttgct gtatcagggg
     9241 gaaccaaaag ccctgacaca aaccatggcc ggacgcagtt ttctgatgac cagtccacac
     9301 gagggcaacc gcaaactatt gcaacgcgcc ttgaaactgc cacaggtcag cgacggcatg
     9361 attcagggga aatcggtacg tctgatcctc aaaaaagagg ccacaccaga cgatattcgc
     9421 catgccgacg ggatgccgga aatcaacatc aacgaaacta cgccgcgttt tgaagatgca
     9481 tttattgatt tgctgggtgg tgccggaacc tcagaatcgc cgctgggcgc aatattacat
     9541 acggtagaag gcacacccgg cgagacggtg atcgaagcga aagaactgac caagaaattt
     9601 ggtgattttg ccgccaccga tcacgtcaac tttgccgtta aacgtgggga gatttttggt
     9661 ttgctggggc caaacggcgc gggtaaatcg accaccttta agatgatgtg cggtttgctg
     9721 gtgccgactt ccggccaggc gctggtgctg gggatggatc tgaaagagag ttctggtaaa
     9781 gcgcgccagc atctcggcta tatggcgcaa aagttttcgc tctacggtaa cctgacggtc
     9841 gaacagaatt tacgcttttt ctctggtgtg tatggcttac gcggtcgggc gcagaacgaa
     9901 aaaatctccc gtatgagcga ggcgttcggc ctgaaaagta tcgcctccca cgccaccgat
     9961 gaactgccat taggttttaa acagcggctg gcactggcct gctcgctgat gcatgaaccg
    10021 gacattctgt ttctcgacga accgacgtcc ggcgttgatc cgattactcg ccgcgaattc
    10081 tggctacata tcaacagcat ggtagagaaa ggcgtcacgg tgatggtcac cacccacttt
    10141 atggatgaag cagaatattg cgaccgcatc ggcctggtgt accgtgggaa attaattgcc
    10201 agcggcacgc cggatgattt gaaagcgcag tcggccaacg acgagcaacc cgatcccacc
    10261 atggagcaag cctttattca gttgatccac gactgggata aggagcatag caatgagtaa
    10321 cccgatcctg tcctggcgtc gcgtacgggc gctgtgcgtt aaagagacgc ggcagatcgt
    10381 tcgcgatccg agtagctggc tgattgcggt agtgatcccg ctgctactgc tgtttatttt
    10441 tggttacggc attaacctcg actccagcaa gctgcgggtc gggattttac tggaacagcg
    10501 tagcgaagcg gcgctggatt tcacccacac catgaccggt tcgccctaca tcgacgccac
    10561 catcagcgat aaccgtcagg aactgatcgc caaaatgcag gcggggaaaa ttcgcggtct
    10621 ggtggttatt ccggtggatt ttgcggaaca gatggagcgc gccaacgcca ccgcaccgat
    10681 tcaggtgatc accgacggca gtgagccgaa taccgctaac tttgtacagg ggtatgtcga
    10741 agggatctgg cagatctggc aaatgcagcg agcggaggac aacgggcaga cttttgaacc
    10801 gcttattgat gtacaaaccc gctactggtt taacccggcg gcgattagcc agcacttcat
    10861 tatccccggt gcggtgacca ttatcatgac ggtcatcggc gcgattctca cctcgctggt
    10921 ggtggcgcga gaatgggaac gcggcaccat ggaggctctg ctctctacgg agattacccg
    10981 cacggaactg ctgctgtgta agctgatccc ttattacttt ctcgggatgc tggcgatgtt
    11041 gctgtgtatg ctggtgtcag tgtttattct cggcgtgccg tatcgcgggt cgctgctgat
    11101 tctgtttttt atctccagcc tgtttttact cagtaccctg gggatggggc tgctgatttc
    11161 cacgattacc cgcaaccagt tcaatgccgc tcaggtcgcc ctgaacgccg cttttctgcc
    11221 gtcgattatg ctttccggct ttatttttca gatcgacagt atgcccgcgg tgatccgcgc
    11281 ggtgacgtac attattcccg ctcgttattt cgtcagcacc ctgcaaagcc tgttcctcgc
    11341 cgggaatatt ccagtggtgc tggtggtaaa cgtgctgttt ttgatcgctt cggcggtgat
    11401 gtttatcggc ctgacgtggc tgaaaaccaa acgtcggctg gattagggag aagagcatgt
    11461 ttcatcgctt atggacgtta atccgcaaag agttgcagtc gctgctgcgc gaaccgcaaa
    11521 cccgcgcgat tctgatttta cctgtgctta ttcaggtgat tctgttcccg ttcgccgcca
    11581 cgctggaagt gactaacgcc accatcgcca tctacgatga agataacggc gagcattcgg
    11641 tggagctgac ccaacgtttt gcccgcgcca gcgcctttac tcatgtgctg ctgctgaaaa
    11701 gcccacagga gatccgccca accatcgaca cacaaaaggc gttactactg gtgcgtttcc
    11761 cggctgactt ctcgcgcaaa ctggatacct tccagaccgc gccgttgcag ttgatcctcg
    11821 acgggcgtaa ctccaacagt gcgcaaattg ccgccaacta cctgcaacag atcgtcaaaa
    11881 attatcagca ggagctgctg gaaggaaaac cgaaacctaa caacagcgag ctggtggtac
    11941 gcaactggta taacccgaat cttgactaca aatggtttgt ggtgccgtcg ctgatcgcca
    12001 tgatcaccac tatcggcgta atgatcgtca cttcactttc cgtcgcccgc gaacgtgaac
    12061 aaggtacgct cgatcagcta ctggtttcgc cgctcaccac ctggcagatc ttcatcggca
    12121 aagccgtacc ggcgttaatt gtcgccacct tccaggccac cattgtgctg gcgattggta
    12181 tctgggcgta tcaaatcccc ttcgccggat cgctggcgct gttctacttt acgatggtga
    12241 tttacggttt atcgctggta ggattcggtc tgttgatttc atcactctgc tcgacacaac
    12301 agcaggcgtt tatcggcgtg tttgttttta tgatgcccgc cattctcctt tccggttacg
    12361 tttctccggt ggaaaacatg ccagtatggc tgcaaaacct gacgtggatt aaccctattc
    12421 gccactttac ggacattacc aagcagattt atttgaagga tgcgagtctg gatattgtgt
    12481 ggaatagttt gtggccgcta ctggtgataa cggccacgac agggtcagcg gcgtacgcga
    12541 tgtttagacg taaggtgatg taacttctta tctttcgcca gcaaagacac taccgccggg
    12601 ccggcaagga ttgccagccc tgcaattgcc agcaaaaagt tgttttccag tacacgggac
    12661 aacagccaca gcacaatcag cgctgcgcca aaataccagg tcgtggtcag ctcttccagc
    12721 acgtctgcca cctcacgcca gcgttgctcg gggtggcgct gaaacaccaa catcatcact
    12781 accgtgacac aatacagtgc gcataatccc acgccgacgt gaaccagagt cttactcatc
    12841 cagcccacca ccagcagcgc cactaccagt aaatgcaaca taatctgcca gcaacttaga
    12901 ccagttgcga cacgaacacg ttgttgccac ttcatggctt ctctcctgaa gaatttatct
    12961 ctacttactc agagtaccga actttacgga aaattccgta agaccgcacc tttttgtgac
    13021 accgactaca ctatttcaat gagataagag gcaaagacag gaaaattatg cccgatcaaa
    13081 cacaacaatt ttcgttcaag gtgctcacca tcaacattca caaaggtttt accgcgttta
    13141 accgacgctt cattttgccg gaacttcgcg acgcggtgcg taccgtcagc gccgatattg
    13201 tttgcctgca ggaagtgatg ggcgcgcacg aagttcatcc gctgcatgtg gaaaactggc
    13261 ccgatacctc gcactacgag tttctcgccg acacgatgtg gagcgatttt gcctacggtc
    13321 gcaatgccgt atacccgaaa gggcatcacg gcaacgccgt actgtcgcgt tatcccattg
    13381 aacattatga gaatcgcgat gtttcggtcg atggtgcgga aaagcgcggc gtgctctact
    13441 gccgcattgt gccaccgatg accggaaaag cgattcatgt gatgtgcgta catctgggcc
    13501 tgcgtgaggc gcaccgtcag gcgcagcttg cgatgctcgc cgaatgggtg aatgagctac
    13561 cggacggcga accggtattg gtggcgggtg atttcaatga ctggcggcaa aaagctaatc
    13621 atccgttaaa agtgcaggcc ggactggatg agatttttac ccgcgcccac ggacgcccgg
    13681 cgcgcacgtt tccggtgcaa tttcctctac tacgactgga caggatctac gtcaaaaatg
    13741 ccagcgccag cgcgccaacc gcgttgccgc tgcggacatg gcgacacctt tctgatcatg
    13801 cccctttaag tgcggagatt catttatgaa atgtagctgg cgcgaaggca ataagatcca
    13861 gttgctggaa aacggcgagc aatattatcc cgcggtgttt aaggcgattg gcgaggcaca
    13921 agaacgcatc attcttgaaa cgtttatctg gtttgaggat gacgtcggca aacagctgca
    13981 tgcggcacta ctggcagcag cgcaacgcgg ggttaaagcg gaagtcttgc tggatggcta
    14041 cggttcgccg gatctcagcg atgagtttgt caatgaactg acggcagctg gcgtggtatt
    14101 tcgctactac gatccccgcc ctcgcctttt tggtatgcgc accaatgtgt ttcgccggat
    14161 gcatcgcaaa attgtggtga tcgacgcgcg tatcgccttt attggcgggc tgaattactc
    14221 cgccgagcat atgtccagct acggtccaga ggctaaacag gattacgcgg tacgccttga
    14281 agggccgatt gtcgaagata ttctccagtt tgagctggaa aacctgcctg gacagagcgc
    14341 ggcacgacgc tggtggcgac gtcatcacaa agcggaagag aaccgccagc cgggagaagc
    14401 gcaggtattg ctggtctggc gcgataacga agaacatcgc gatgatattg aacgtcacta
    14461 tctgaaaatg ctcactcagg cgcggcgaga agtcattatc gccaacgcct acttcttccc
    14521 cggctatcga tttttacacg ccttgcgtaa agcggcacgg cgcggggtgc ggatcaaact
    14581 gatcattcag ggcgaaccgg atatgccgat tgtcagagtc ggtgcgcgct tgctgtataa
    14641 ctatctggtt aaaggcggcg ttcaggtatt tgagtaccgc cgccgcccgc tccacggcaa
    14701 agtggcattg atggacgatc actgggcgac agtagggtcc agtaatctcg atccgctcag
    14761 tttgtcactg aatctcgaag caaatgtcat catccacgat cgtcatttta accagacgct
    14821 gcgcgataat ctgaacggca ttattgccgc agattgtcag caggtcgatg agaccatgct
    14881 gccgaaacgc acctggtgga acctgaccaa aagcgtgctg gcgttccact ttttacgcca
    14941 cttcccggcg ctggttggct ggcttccggc acacacacca cgtctggcgc aggttgatcc
    15001 gcccgcacaa ccgacaatgg aaacgcagga tcgggtagaa actgaaaaca cgggggtaaa
    15061 accctgatga gtaaatcaca cccgcgctgg cgcttagcaa agaagatcct cacctggctg
    15121 ttttttatcg cggtgattgt gttgctggtg gtctacgcca aaaaagtgga ctgggaagag
    15181 gtctggaagg tcatccgcga ctacaatcgc gttgcgctgc ttagtgcggt cgggctggtg
    15241 gtcgtcagct atctgattta cggctgctat gacctgctcg cccgctttta ctgcggtcac
    15301 aaactggcga agcgccaggt gatgctggtg tcgtttatct gctacgcctt caacctgacg
    15361 ctcagtacct gggtcggcgg cattggtatg cgctatcgtt tgtactcccg actagggtta
    15421 ccgggcagca ctattacgcg gattttctcg ctcagtatta ccaccaactg gctgggctat
    15481 attttactgg cagggattat ctttaccgca ggcgtggtgg agttgccgga tcactggtat
    15541 gtcgatcaaa ctacgctgcg tattctcggc attggcttac tgatgattat cgcggtttat
    15601 ttgtggtttt gcgctttcgc gaagcaccgc catatgacca tcaaagggca aaagctggtg
    15661 ctgccttcat ggaaattcgc cctcgctcag atgctgattt ccagcgttaa ctggatggta
    15721 atgggggcga ttatctggct gttacttggt caaagcgtga actatttctt tgtactgggc
    15781 gtgttactgg ttagtagtat tgctggcgtc atcgtgcata ttccggtggg gatcggtgtg
    15841 ctggaagcgg tgtttatcgc actactggct ggggagcata cctcgaaagg gagcattatc
    15901 gccgccctgc tcgcttatcg cgtcctgtat tactttatcc cgctgctgct ggcgctgatc
    15961 tgttacctgc tactggaaag ccaggcgaag aagctacggg cgaaaaatga agcggcgatg
    16021 tgaagtttag ttaagttctt tagtatctgc atttacgact aatgaaaaaa acgcgtatgc
    16081 ctttgccaga caagcgttat agctatcgcg gcaagtatcc caaagaagcc gataaaatag
    16141 taatagagtg ttaatgcacc tgtaacagcg caacgaaccg gactccacaa ctcttcacta
    16201 tggcatttcc ccgccaattc aaggagctta tttcgtccat gcgagataat gcctgaccat
    16261 aacacaatca ttaagcaact tattacccaa acgggtcgtt cactcattaa tgcggcattc
    16321 atgatgatca ccagggccaa ccctgtcaac gtcatcataa tgataaaacg gtgagaacca
    16381 ggatcaacat taaataataa accggccagc atactgatta aaaacatcgc gcccgcagtg
    16441 ctgaacacga cgataatggc aggtacagga gctaaacttg caacgacaat cgccaccaaa
    16501 gagagtaaaa acagatatat tacgcctgcg atgacggcat ttatcttgtt gatatcctta
    16561 cttaagccat acatacaaac taataataac cctatggcga catacattgt tggtgaatca
    16621 tatttggttg ccgtaatttc gagatactta gctccccagg ccagtcctgc ggttgccacc
    16681 aaacctaaag cggtaatgag ccatataccg tttaatatcc tggtctcgtg ctgaaaatct
    16741 aatttattgc tgttttgtga gtatg
//
LOCUS       DAZKHF020000082        16511 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2201S31241
            SAMN43267800-rid25106413.denovo.082, whole genome shotgun sequence.
ACCESSION   DAZKHF020000082 DAZKHF020000000
VERSION     DAZKHF020000082.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267800
            Sequence Read Archive: SRR30307263
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 16511)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16511)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 16511)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350581.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350581.2
            Genome Coverage       :: 151x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:21:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,652
            CDSs (total)                      :: 5,557
            Genes (coding)                    :: 5,320
            CDSs (with protein)               :: 5,320
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 237
            CDSs (without protein)            :: 237
            Pseudo Genes (ambiguous residues) :: 0 of 237
            Pseudo Genes (frameshifted)       :: 102 of 237
            Pseudo Genes (incomplete)         :: 130 of 237
            Pseudo Genes (internal stop)      :: 52 of 237
            Pseudo Genes (multiple problems)  :: 41 of 237
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16511
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267800-rid25106413.denovo.082"
                     /serotype="O 26:H11"
                     /isolate="2201S31241"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-01"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            <1..258
                     /locus_tag="ACHOSC_RS11245"
                     /old_locus_tag="pgaptmp_002249"
     CDS             <1..258
                     /locus_tag="ACHOSC_RS11245"
                     /old_locus_tag="pgaptmp_002249"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3'-5' exonuclease"
                     /protein_id="HGU4548179.1"
                     /translation="WGNGANFDNTILRRSYERQGIPCPWRYYNDRDVRTIVELGKAID
                     FDARTAIPFEGERHNALDDARHQAKYVSAIWQKLIPNQADF"
     gene            320..589
                     /locus_tag="ACHOSC_RS11250"
                     /old_locus_tag="pgaptmp_002250"
     CDS             320..589
                     /locus_tag="ACHOSC_RS11250"
                     /old_locus_tag="pgaptmp_002250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excisionase"
                     /protein_id="HGU4548180.1"
                     /translation="MSEQYLITLDEWKPKRFSLPITNTTLVKYGKLGYIVPRPQKIRG
                     RWLIDRRAVFVGPGETGIAPEIHTGDDDALKEILTHVTEATKKQH"
     gene            558..1676
                     /locus_tag="ACHOSC_RS11255"
                     /old_locus_tag="pgaptmp_002251"
     CDS             558..1676
                     /locus_tag="ACHOSC_RS11255"
                     /old_locus_tag="pgaptmp_002251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008907 - integrase activity [Evidence
                     IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage integrase Arm DNA-binding
                     domain-containing protein"
                     /protein_id="HGU4548181.1"
                     /translation="MSPRPRKNSTDVAGLYEKFDRRTGRVYYQYKNPVTGKFHGLGTD
                     KGKAEKIASTANQRIAAAEAEYFMRKIDESPSATKRRGIRLKAWVDRYLKIQDTRLKN
                     GDIAATTHKEKTRMAAYLVSRLGNHPLKELEVRDFALILDEWLDKDMVSTARVNRGLW
                     VDIYKEAQHAGEVPPGWNPPEATRKPIPKVTRARLTMEDWQKIYNATPEKHFIRNAML
                     LAIVTGQRRDDICHMRFSDVWNEHLHITQGKTGMRLALPLTLRCDAIGITLKEVIDGC
                     RDRILSPYLIHSRHQKQPKPMSKDNLSDYFAKARDLAGIIPPAGKTPPTFHEQRSLSE
                     RLYRAQGIDTKTLLGHKVQATTDRYNDTRGQEWVKLVI"
     gene            1843..2637
                     /gene="potC"
                     /locus_tag="ACHOSC_RS11260"
                     /old_locus_tag="pgaptmp_002252"
     CDS             1843..2637
                     /gene="potC"
                     /locus_tag="ACHOSC_RS11260"
                     /old_locus_tag="pgaptmp_002252"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="spermidine/putrescine ABC transporter permease
                     PotC"
                     /protein_id="HGU4548182.1"
                     /translation="MIGRLLRGGFMTAIYAYLYIPIIILIVNSFNSSRFGINWQGFTT
                     KWYSLLMNNDSLLQAAQHSLTMAVFSATFATLIGSLTAVALYRYRFRGKPFVSGMLFV
                     VMMSPDIVMAISLLVLFMLLGIQLGFWSLLFSHITFCLPFVVVTVYSRLKGFDVRMLE
                     AAKDLGASEFTILRKIILPLAMPAVAAGWVLSFTLSMDDVVVSSFVTGPSYEILPLKI
                     YSMVKVGVSPEVNALATILLVLSLVMVIASQLIARDKTKGNTGDVK"
     gene            2634..3680
                     /gene="potD"
                     /locus_tag="ACHOSC_RS11265"
                     /old_locus_tag="pgaptmp_002253"
     CDS             2634..3680
                     /gene="potD"
                     /locus_tag="ACHOSC_RS11265"
                     /old_locus_tag="pgaptmp_002253"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA];
                     GO_function: GO:0019808 - polyamine binding [Evidence
                     IEA];
                     GO_process: GO:0015846 - polyamine transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="spermidine/putrescine ABC transporter
                     substrate-binding protein PotD"
                     /protein_id="HGU4548183.1"
                     /translation="MKKWSRHLLAAGALALGMSAAHADDNNTLYFYNWTEYVPPGLLE
                     QFTKETGIKVIYSTYESNETMYAKLKTYKDGAYDLVVPSTYYVDKMRKEGMIQKIDKS
                     KLTNFSNLDPDMLNKPFDPNNDYSIPYIWGATAIGVNGDAVDPKSVTSWADLWKPEYK
                     GSLLLTDDAREVFQMALRKLGYSGNTTDPKEIEAAYNELKKLMPNVAAFNSDNPANPY
                     MEGEVNLGMIWNGSAFVARQAGTPIDVVWPKEGGIFWMDSLAIPANAKNKEGALKLIN
                     FLLRPDVAKQVAETIGYPTPNLAARKLLSPEVANDKTLYPDAETIKNGEWQNDVGAAS
                     SIYEEYYQKLKAGR"
     gene            3738..4199
                     /locus_tag="ACHOSC_RS11270"
                     /old_locus_tag="pgaptmp_002254"
     CDS             3738..4199
                     /locus_tag="ACHOSC_RS11270"
                     /old_locus_tag="pgaptmp_002254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001442351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3592 domain-containing protein"
                     /protein_id="HGU4548184.1"
                     /translation="MSQDSKVLSRAFLGIGLILLVISAIIFYYHFTFTKSAVHTEGII
                     VDAVWYNNHSNDVDDNGSWYPVVAFRPTPDYTLIFNSNIGSDFYEDSEGDRVNVYYPP
                     GHPEQAEINNPWVNFFKWGFVGIAGIIFGSVGLIITLPSTKKSRRKRKSCP"
     gene            4196..4984
                     /locus_tag="ACHOSC_RS11275"
                     /old_locus_tag="pgaptmp_002255"
     CDS             4196..4984
                     /locus_tag="ACHOSC_RS11275"
                     /old_locus_tag="pgaptmp_002255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YgcG family protein"
                     /protein_id="HGU4548185.1"
                     /translation="MKKFIILLSLLILLPLVATSKPLIPIMKTLFTDVTGTVPDAEEI
                     ARKAELFRQQTGIAPFIVILPDINNEASLRQNGKAMLAHASSSLSNVKGSVLLLFTTR
                     EPRLIMITNGQVESGLDDKHLGLLIENHTLAYLNADLWYQGINNALAVLQAQILKQPT
                     PPLTYYPHPGQQHENAPPGSTNTLGFIAWAATFILFSWIFCYTTRFIYALKFAVAMTI
                     ANMGYQALCLYIDNSFAITRISPLWAGLIGVCTFIAALLLTSKR"
     gene            complement(5123..5944)
                     /gene="cobB"
                     /locus_tag="ACHOSC_RS11280"
                     /old_locus_tag="pgaptmp_002256"
     CDS             complement(5123..5944)
                     /gene="cobB"
                     /locus_tag="ACHOSC_RS11280"
                     /old_locus_tag="pgaptmp_002256"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Sir2 family NAD+-dependent deacetylase"
                     /protein_id="HGU4548186.1"
                     /translation="MLSRRGHRLSRFRKNKRRLRERLRQRIFFRDKVVPEAMEKPRVL
                     VLTGAGISAESGIRTFRAADGLWEEHRVEDVATPEGFDRDPELVQAFYNARRRQLQQP
                     EIQPNAAHLALAKLQDALGDRFLLVTQNIDNLHERAGNTNVIHMHGELLKVRCSQSGQ
                     VLDWTGDVTPEDKCHCCQFPAPLRPHVVWFGEMPLGMDEIYMALSMADIFIAIGTSGH
                     VYPAAGFVHEAKLHGAHTVELNLEPSQVGNEFAEKYYGPASQVVPEFVEKLLKGL"
     gene            complement(5960..6871)
                     /gene="nagK"
                     /locus_tag="ACHOSC_RS11285"
                     /old_locus_tag="pgaptmp_002257"
     CDS             complement(5960..6871)
                     /gene="nagK"
                     /locus_tag="ACHOSC_RS11285"
                     /old_locus_tag="pgaptmp_002257"
                     /EC_number="2.7.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0045127 - N-acetylglucosamine kinase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylglucosamine kinase"
                     /protein_id="HGU4548187.1"
                     /translation="MYYGFDIGGTKIALGVFDSGRQLQWEKRVPTPRDSYDAFLDAVC
                     ELVAEADQRFGCKGSVGIGIPGMPETEDGTLYAANVPAASGKPLRADLSARLDRDVRL
                     DNDANCFALSEAWDDEFTQYPLVMGLILGTGVGGGLIFNGKPITGKSYITGEFGHMRL
                     PVDALTMMGLDFPLRRCGCGQHGCIENYLSGRGFAWLYQHYYHQPLQAPEIIALYDQG
                     DEQARAHVERYLDLLAVCLGNILTIVDPDLVVIGGGLSNFPAITTQLADRLPRHLLPV
                     ARVPRIERARHGDAGGMRGAAFLHLTD"
     gene            complement(6900..8144)
                     /gene="lolE"
                     /locus_tag="ACHOSC_RS11290"
                     /old_locus_tag="pgaptmp_002258"
     CDS             complement(6900..8144)
                     /gene="lolE"
                     /locus_tag="ACHOSC_RS11290"
                     /old_locus_tag="pgaptmp_002258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0042953 - lipoprotein transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein-releasing ABC transporter permease
                     subunit LolE"
                     /protein_id="HGU4548188.1"
                     /translation="MAMPLSLLIGLRFSRGRRRGGMVSLISVISTIGIALGVAVLIVG
                     LSAMNGFERELNNRILAVVPHGEIEAVDQPWTNWQEALDHVQKVPGIAAAAPYINFTG
                     LVESGANLRAIQVKGVNPQQEQRLSALPSFVQGDAWRNFKAGEQQIIIGKGVADALKV
                     KQGDWVSIMIPNSNPEHKLMQPKRVRLHVAGILQLSGQLDHSFAMIPLADAQQYLDMG
                     SSVSGIALKMTDVFNANKLVRDAGEVTNSYVYIKSWIGTYGYMYRDIQMIRAIMYLAM
                     VLVIGVACFNIVSTLVMAVKDKSGDIAVLRTLGAKDGLIRAIFVWYGLLAGLFGSLCG
                     VIIGVVVSLQLTPIIEWIEKLIGHQFLSSDIYFIDFLPSELHWLDVFYVLVTALLLSL
                     LASWYPARRASNIDPARVLSGQ"
     gene            complement(8144..8845)
                     /gene="lolD"
                     /locus_tag="ACHOSC_RS11295"
                     /old_locus_tag="pgaptmp_002259"
     CDS             complement(8144..8845)
                     /gene="lolD"
                     /locus_tag="ACHOSC_RS11295"
                     /old_locus_tag="pgaptmp_002259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415635.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0042953 - lipoprotein transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein-releasing ABC transporter
                     ATP-binding protein LolD"
                     /protein_id="HGU4548189.1"
                     /translation="MNKILLQCDNLCKRYQEGSVQTDVLHNVSFSVGEGEMMAIVGSS
                     GSGKSTLLHLLGGLDTPTSGDVIFNGQPMSKLSSAAKAELRNQKLGFIYQFHHLLPDF
                     TALENVAMPLLIGKKKPAEINSRALEMLKAVGLDHRANHRPSELSGGERQRVAIARAL
                     VNNPRLVLADEPTGNLDARNADSIFQLLGELNRLQGTAFLVVTHDLQLAKRMSRQLEM
                     RDGRLTAELSLMGAE"
     gene            complement(8838..10037)
                     /gene="lolC"
                     /locus_tag="ACHOSC_RS11300"
                     /old_locus_tag="pgaptmp_002260"
     CDS             complement(8838..10037)
                     /gene="lolC"
                     /locus_tag="ACHOSC_RS11300"
                     /old_locus_tag="pgaptmp_002260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004976588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0042953 - lipoprotein transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein-releasing ABC transporter permease
                     subunit LolC"
                     /protein_id="HGU4548190.1"
                     /translation="MYQPVALFIGLRYMRGRAADRFGRFVSWLSTIGITLGVMALVTV
                     LSVMNGFERELQNNILGLMPQAILSSEHGSLNPQQLPETAVKLDGVNRVAPITTGDVV
                     LQSARSVAVGVMLGIDPAQKDPLTPYLVNVKQTDLEPGKYNVILGEQLASQLGVNRGD
                     QIRVMVPSASQFTPMGRIPSQRLFNVIGTFAANSEVDGYEMLVNIEDASRLMRYPAGN
                     ITGWRLWLDEPLKVDSLSQQKLPEGSKWQDWRDRKGELFQAVRMEKNMMGLLLSLIVA
                     VAAFNIITSLGLMVMEKQGEVAILQTQGLTPRQIMMVFMVQGASAGIIGAILGAALGA
                     LLASQLNNLMPIIGVLLDGAALPVAIEPLQVIVIALVAMAIALLSTLYPSWRAAATQP
                     AEALRYE"
     gene            10299..11372
                     /locus_tag="ACHOSC_RS11305"
                     /old_locus_tag="pgaptmp_002261"
     CDS             10299..11372
                     /locus_tag="ACHOSC_RS11305"
                     /old_locus_tag="pgaptmp_002261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase family protein"
                     /protein_id="HGU4548191.1"
                     /translation="MKQKELWINQIKGLCICLVVIYHSVITFYPHLTTFQHPLSEVLS
                     KCWIYFNLYLAPFRMPVFFFISGYLIRRYIDSVPWGNCLDKRIWNIFWVLALWGVVQW
                     LALSALNQWLAPERDLSNASNAAYADSTGEFLHGMITASTSLWYLYALIVYFVVCKIF
                     SRLALPLFALFVLLSVAVNFVPTPWWGMNSVIRNLLYYSLGAWFGATIMTCVKEVPLR
                     RHLLMASLLTVLAVGAWLFTISLLLSLVSIVVIMKLFYQYEQRFGMRSTSLLNVIGSN
                     TIAIYTTHRILVEIFSLTLLAQMNAARWSPQVELTLLLVYPFVSLFICTVAGLLVRKL
                     SQRAFSDLLFSPPSLPAAVSYSR"
     gene            11500..14946
                     /gene="mfd"
                     /locus_tag="ACHOSC_RS11310"
                     /old_locus_tag="pgaptmp_002262"
     CDS             11500..14946
                     /gene="mfd"
                     /locus_tag="ACHOSC_RS11310"
                     /old_locus_tag="pgaptmp_002262"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003684 - damaged DNA binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription-repair coupling factor"
                     /protein_id="HGU4548192.1"
                     /translation="MPEQYRYTLPVKAGEQRLLGELTGAACATLVAEIAERHAGPVVL
                     IAPDMQNALRLHDEISQFTDQMVMNLADWETLPYDSFSPHQDIISSRLSTLYQLPTMQ
                     RGVLIVPVNTLMQRVCPHSFLHGHALVMKKGQRLSRDALRTQLDSAGYRHVDQVMEHG
                     EYATRGALLDLFPMGSELPYRLDFFDDEIDSLRVFDVDSQRTLEEVEAINLLPAHEFP
                     TDKAAIELFRSQWRDTFEVKRDPEHIYQQVSKGTLPAGIEYWQPLFFSEPLPPLFSYF
                     PANTLLVNTGDLETSAERFQADTLARFENRGVDPMRPLLPPQSLWLRVDELFSELKNW
                     PRVQLKTEHLPTKAANANLGFQKLPDLAVQAQQKAPLDALRKFLETFDGPVVFSVESE
                     GRREALGELLARIKIAPQRIMRLDEASDRGRYLMIGAAEHGFVDKVRNLALICESDLL
                     GERVARRRQDSRRTINPDTLIRNLAELHIGQPVVHLEHGVGRYAGMTTLEAGGITGEY
                     LMLTYANDAKLYVPVSSLHLISRYAGGAEENAPLHKLGGDAWSRARQKAAEKVRDVAA
                     ELLDIYAQRAAKEGFAFKHDREQYQLFCDSFPFETTPDQAQAINAVLSDMCQPLAMDR
                     LVCGDVGFGKTEVAMRAAFLAVDNHKQVAVLVPTTLLAQQHYDNFRDRFANWPVRIEM
                     ISRFRSAKEQTQILAEVAEGKIDILIGTHKLLQSDVKFKDLGLLIVDEEHRFGVRHKE
                     RIKAMRANVDILTLTATPIPRTLNMAMSGMRDLSIIATPPARRLAVKTFVREYDSLVV
                     REAILREILRGGQVYYLYNDVENIQKAAERLAELVPEARIAIGHGQMRERELERVMND
                     FHHQRFNVLVCTTIIETGIDIPTANTIIIERADHFGLAQLHQLRGRVGRSHHQAYAWL
                     LTPHPKAMTTDAQKRLEAIASLEDLGAGFALATHDLEIRGAGELLGEEQSGSMETIGF
                     SLYMELLENAVDALKAGREPSLEDLTSQQTEVELRMPSLLPDDFIPDVNTRLSFYKRI
                     ASAKTENELEEIKVELIDRFGLLPDPARTLLDVARLRQQAQKLGIRKLEGNEKGGVIE
                     FAEKNHVNPAWLIGLLQKQPQHYRLDGPTRLKFIQDLSERKTRIEWVRQFMRELEENA
                     IA"
     gene            15090..16052
                     /gene="ldtC"
                     /locus_tag="ACHOSC_RS11315"
                     /old_locus_tag="pgaptmp_002263"
     CDS             15090..16052
                     /gene="ldtC"
                     /locus_tag="ACHOSC_RS11315"
                     /old_locus_tag="pgaptmp_002263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase LdtC"
                     /protein_id="HGU4548193.1"
                     /translation="MMIKTRFSRWLTFFTFAAAVALALPAKANTWPLPPAGSRLVGEN
                     KFHVVENDGGSLEAIAKKYNVGFLALLQANPGVDPYVPRAGSVLTIPLQTLLPDAPRE
                     GIVINIAELRLYYYPPGKNSVTVYPIGIGQLGGDTLTPTMVTTVSDKRANPTWTPTAN
                     IRARYKAQGIELPAVVPAGPDNPMGHHAIRLAAYGGVYLLHGTNADFGIGMRVSSGCI
                     RLRDDDIKTLFSQVTPGTKVNIINTPIKVSAEPNGARLVEVHQPLSEKIDDDPQLLPI
                     TLNSAMQSFKDAAQTDAEVMQHVMDVRSGMPVDVRRHQVSPQTL"
     gene            complement(16135..16392)
                     /gene="bhsA"
                     /locus_tag="ACHOSC_RS11320"
                     /old_locus_tag="pgaptmp_002264"
                     /gene_synonym="ycfR"
     CDS             complement(16135..16392)
                     /gene="bhsA"
                     /locus_tag="ACHOSC_RS11320"
                     /old_locus_tag="pgaptmp_002264"
                     /gene_synonym="ycfR"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multiple stress resistance protein BhsA"
                     /protein_id="HGU4548194.1"
                     /translation="MKNVKTLIAAAILSSMSFASFAAVEVQSTPEGQQKVGTISANAG
                     TNLGSLEEQLAQKADEMGAKSFRITSVTGPNTLHGTAVIYK"
BASE COUNT         4183 a         4239 c         4143 g         3946 t
ORIGIN      
        1 tggggaaatg gagccaactt cgacaacacg attttgcgcc gttcatacga acggcagggg
       61 atcccctgcc cgtggcgtta ctacaacgat cgcgatgtac gcacaatcgt tgagctgggg
      121 aaagccatag acttcgatgc cagaacggct attccattcg aaggtgagcg ccataatgca
      181 cttgatgacg ctcgtcacca ggcaaaatac gtttcagcta tctggcaaaa actgatcccg
      241 aatcaggctg atttttaatg ttcaaccgtc gccagttgtc gttgatattc tgcaactggc
      301 gcgttccgga gtgatagcca tgagcgaaca gtacctgata acgctcgacg agtggaaacc
      361 aaaacggttc agtctcccaa taacaaacac taccctggtg aaatacggaa aactaggata
      421 catcgttcca agaccacaaa aaattcgtgg gcgttggctg atagatcgcc gagcagtatt
      481 tgttgggcct ggtgaaacgg gaattgcgcc ggaaattcat actggcgatg atgatgcact
      541 gaaggagatt ttaactcatg tcaccgaggc cacgaaaaaa cagcactgac gtagccggtc
      601 tttacgaaaa gtttgatcgc agaactggca gagtttacta ccagtataaa aatcctgtga
      661 ctggaaaatt tcacggactc ggaacagaca aaggtaaggc agaaaaaatc gcttccacag
      721 ccaatcagcg aatagctgca gcagaagctg aatatttcat gcgcaaaatt gatgaaagtc
      781 cgtcagcaac aaaacgtcgg ggtatcagat taaaggcatg ggttgatcga tatctgaaaa
      841 tacaggacac gcgactgaaa aatggagata ttgcagctac aactcacaaa gaaaaaaccc
      901 gaatggctgc atacctggtt tcccgtctgg gaaaccaccc attgaaagaa ctggaagtaa
      961 gagactttgc attaatactg gatgagtggc tggataaaga catggtcagc acagcgagag
     1021 taaatcgtgg attatgggtt gatatttata aagaagcaca gcatgcaggg gaagttcctc
     1081 ctggatggaa tcctccggag gctacccgta aaccgatccc taaagtaacc agagccaggc
     1141 tcaccatgga agactggcaa aaaatttaca atgcaacgcc tgaaaaacac tttatccgta
     1201 acgcaatgct tcttgcgatt gttactggtc agcgccgtga tgacatttgc cacatgcgtt
     1261 tttcagatgt gtggaacgaa cacttgcata tcacccaggg aaaaaccgga atgcgtctgg
     1321 cgttaccgct tacactacgc tgtgatgcca ttgggataac gttaaaagaa gttattgatg
     1381 ggtgccgaga cagaatatta agtccatatc taatccatag tcggcaccag aaacaaccga
     1441 agccgatgag taaagacaac ctgagcgact actttgccaa agcacgggat ctggctggga
     1501 taattccacc agcaggaaaa actccgccaa catttcatga acaacgctct ctatcagaac
     1561 ggctgtaccg tgcacagggt attgatacaa aaacattact aggacataaa gtccaggcaa
     1621 ccaccgatcg ctataacgat actcgaggtc aggaatgggt taagttggtt atttgataaa
     1681 caccatatgg aatatccgtc accatctcag gaaaaaagtg ttgtaaaatg cgtggcctag
     1741 ttttggggag acgttttgga gagattttgg agaaaggaaa aaattgaata aattcaaaat
     1801 cctgaaagtg tcccgcctta tgaataaaaa ggtggaactc gaatgatcgg tcgactgctt
     1861 cgcggcggtt ttatgaccgc tatctacgcg tacctgtata tcccaatcat tattttgatt
     1921 gtgaactcct ttaacagctc gcgctttggc atcaactggc agggttttac caccaaatgg
     1981 tatagcctgc tgatgaacaa cgacagcctg ttacaggcag cgcagcattc actaacaatg
     2041 gcggtgtttt cggcgacatt tgctacgctt atcggttcac tgacggcagt tgcgctgtac
     2101 cgttatcgct ttcgtggtaa gccgttcgtt agcggaatgc tgtttgtggt gatgatgtca
     2161 ccagatatcg tgatggcgat ttctctgctg gtgctgttta tgctgctggg tattcagctt
     2221 ggcttctggt cgctgctgtt ctcgcatatc accttctgcc tgccgtttgt ggtggtgacg
     2281 gtgtattcgc gcctgaaagg ttttgacgtg cggatgctgg aagcggcgaa agatctcggt
     2341 gccagtgaat ttaccattct gcgaaaaatc attctgccac tggcaatgcc tgcggtggcg
     2401 gcgggctggg tgttaagctt taccctgtcg atggacgatg tggtagtttc ttcgttcgtc
     2461 accggaccaa gttatgaaat tctgccgtta aaaatttatt cgatggtcaa agtcggcgta
     2521 tcgccggaag ttaacgcgct ggcaaccata ttactggtgc tgtcgctggt gatggttatt
     2581 gccagccagc ttattgctcg tgataaaacg aaaggtaaca caggggacgt taaatgaaaa
     2641 aatggtcacg ccacctgctc gcggcgggtg ctctggcact gggcatgagc gccgctcacg
     2701 ccgatgacaa caacacgctg tatttctaca actggaccga gtacgtgccg ccaggactgc
     2761 ttgaacagtt caccaaagaa accggtatta aggttatcta ttcgacttac gagtcgaacg
     2821 aaaccatgta cgccaagctg aaaacgtaca aagacggtgc ctatgacctg gtggttcctt
     2881 caacctatta cgtcgataaa atgcgtaaag aagggatgat ccagaagatc gacaagtcga
     2941 agttaacgaa cttcagcaat ctcgatccag acatgctcaa caagccgttt gacccgaata
     3001 acgattactc cattccgtat atctggggtg cgacggcgat tggtgttaac ggtgatgcgg
     3061 tggatccgaa atctgtcacc agctgggccg atctgtggaa gcctgagtac aaaggcagcc
     3121 tgctgttgac cgacgatgcc cgtgaagtgt tccagatggc gctgcgtaag ctgggctact
     3181 ccggtaacac caccgatccg aaagagattg aagctgcata taacgagctg aaaaaactga
     3241 tgccaaacgt cgcagcgttt aactccgata acccagctaa cccgtacatg gaaggcgaag
     3301 ttaacctcgg catgatctgg aacggttctg ctttcgttgc acggcaggcg ggtacgccaa
     3361 ttgacgtggt gtggccgaaa gaaggcggca ttttctggat ggacagcctg gcgatcccgg
     3421 caaatgccaa aaacaaagaa ggcgcgctga aattgatcaa cttcctgctg cgcccggatg
     3481 tggcaaaaca ggttgctgaa actatcggtt atccaacgcc aaaccttgcg gcgcgtaagc
     3541 tgttaagccc ggaagtggcg aacgataaaa cgctctaccc ggatgctgaa accattaaaa
     3601 atggtgaatg gcagaatgac gttggcgcag ccagcagcat ttatgaagag tattatcaga
     3661 agctgaaagc aggacgttaa ttcagcaagc gggcggtggc attaccgccc gttttttatt
     3721 tagcaaggat gtggctaatg agtcaggaca gtaaagtgtt atcccgcgct tttctcggta
     3781 tcggtctgat attgctcgtt atttctgcaa tcatctttta ttaccatttt acttttacca
     3841 aaagcgcggt tcatacagaa ggaatcattg ttgatgccgt ttggtacaac aatcactcaa
     3901 acgacgtcga tgataatggt tcctggtatc cagtggttgc ctttcgccct acgccagatt
     3961 acacgctaat tttcaattcg aatatcggta gtgattttta tgaagatagc gaaggtgaca
     4021 gagtcaatgt ctattatccg ccagggcatc cagaacaagc cgaaataaac aatccctggg
     4081 ttaatttttt taaatggggc tttgttggca tagcaggcat tattttcggt agtgtcggtc
     4141 tgataatcac gctcccctcc accaaaaaat ctcgcaggaa acggaagtcc tgcccatgaa
     4201 aaaattcatt atactgctca gtttactgat actcctgcct ctggtagcaa ccagtaaacc
     4261 attgattcca ataatgaaga cactttttac ggatgttacc ggtactgtac cggatgctga
     4321 agagatcgca cgtaaagccg agctttttcg ccaacaaacg ggaattgcac cgtttattgt
     4381 gattttaccc gacattaata atgaagccag tctcagacaa aatggtaaag cgatgctggc
     4441 gcatgcgtca tcttcattga gtaatgtaaa aggaagtgtt ctgttactat ttactacccg
     4501 cgaaccacgg ttaattatga tcaccaacgg ccaggttgaa agtggtctgg acgataaaca
     4561 tctcggcctt ctgatagaaa atcacacgct ggcttattta aatgcagatc tctggtatca
     4621 gggaatcaat aatgcattgg ctgttctaca agcacagata ttaaaacaac cgacgccgcc
     4681 actgacgtat tatccgcatc caggacaaca gcatgaaaat gctccccccg gcagcaccaa
     4741 cacattaggt tttatcgcct gggctgcgac gtttatctta ttttcctgga tcttttgcta
     4801 cacgactcgt tttatttatg cgttaaaatt tgcagtggcg atgactattg ccaatatggg
     4861 ttatcaggct ttatgtttgt atatcgataa tagctttgcg attaccagaa tatcgccact
     4921 ctgggcaggg ctaattggtg tctgtacgtt tattgccgca ttgttattga cgagtaaacg
     4981 ataaaagagg ccggaaaagt gcgcgtgtat tatgccggat ggggcgcgaa cgccttatcc
     5041 ggcccacaaa acccgcaaat tcaattaatt gcgtcccctt gtaggcctga taagcgtagc
     5101 gcatcaggca atgcttcctg ctttataatc ccttcagcaa cttttcaaca aactcaggca
     5161 ccacctggct tgccgggccg taatatttct cggcaaattc attaccgacc tgacttggtt
     5221 cgagattcag ttccacggtg tgcgcgccat gcagtttcgc ttcgtgaaca aacccagccg
     5281 ccggataaac atgcccggaa gtaccaatgg caatgaaaat atcagccatc gacaacgcca
     5341 tataaatttc atccatgccg agtggcattt cgccaaacca aacgacgtgc gggcgcaagg
     5401 gtgccgggaa ctggcaacaa tggcatttat cttctggcgt aacgtctccg gtccagtcga
     5461 gaacctgacc actttgcgaa caacgaactt tcagcaattc cccatgcata tgaatgacat
     5521 tggtattacc tgcgcgttca tgcaggttgt ctatattctg cgttaccagc aaaaagcgat
     5581 cgccgagggc gtcttgcagt ttagccagcg caagatgcgc ggcgttaggc tgaatttctg
     5641 gctgctgcag ctgtcgacga cgggcgttat aaaacgcttg caccagttca ggatcgcgat
     5701 cgaaaccttc cggcgttgcc acatcttcaa cccgatgttc ttcccacagg ccatctgcgg
     5761 cgcgaaaggt acgaatacct gattccgcag aaatccctgc ccctgtcagt acgagtactc
     5821 ttggtttttc cattgcttcc ggcaccactt tatctctgaa aaaaatacgc tgacgcaaac
     5881 gctcgcgcag gcggcgttta tttttacgaa aacgacttaa ccgatgaccc cgacgcgaca
     5941 gcatagcaac ctctgttgtt taatcggtta gatgtaggaa ggccgcacca cgcattcctc
     6001 ccgcatcacc gtggcgcgcg cgttcaatgc gcggaacacg agctacaggt aagagatgac
     6061 gaggcagcct gtccgccagt tgcgttgtga ttgccgggaa attcgataag ccaccaccaa
     6121 tgacgaccag gtcagggtca acaatggtca ggatatttcc cagacaaacc gctaataaat
     6181 ccagataacg ctcaacgtgc gcccttgcct gctcatcgcc ttgatcataa agcgcaataa
     6241 tttcgggagc ctgcaacggt tgatgataat agtgttgata cagccacgca aaaccgcgac
     6301 cagacagata attttcaatg cagccatgct gaccacagcc gcagcggcgt aacgggaaat
     6361 ccaaccccat catggttaac gcatcaaccg gcagacgcat atggccaaac tcgccggtaa
     6421 tgtagctttt accggtaatc ggtttgccgt tgaaaatcag cccgccgcca acgccggtgc
     6481 cgagaatcaa ccccatcacc agtggatatt gagtaaattc atcatcccag gcttctgaaa
     6541 gggcaaaaca gttggcatcg ttatcaaggc gtacatcgcg atcaagacgt gcgctcaggt
     6601 cggcacgcag cggtttaccg ctggcagcgg ggacattggc ggcatacagc gtaccatctt
     6661 ctgtttccgg cattcccgga ataccgatgc cgacagaacc tttacagcca aaacgttggt
     6721 cagcttcagc taccagctca cacactgcat ctaaaaatgc gtcatagctg tcacgcggtg
     6781 tcggcacccg cttttcccac tgcaactgcc gaccgctatc aaacacgcca agcgcaattt
     6841 ttgttccacc aatatcaaac ccgtaataca ttgccgctcc ttgttttaat gtactgcctt
     6901 tactggccgc taaggactcg cgcagggtca atattactgg cgcgccgcgc cggataccaa
     6961 cttgccaaaa gactcagcaa caatgctgtg accagtacgt agaagacgtc cagccagtgc
     7021 aattccgatg gcaggaaatc aataaaatag atatcgctgg agaggaactg atgaccgatc
     7081 aacttttcaa tccactcaat aatcggggta agttgcagtg aaaccaccac accgataatc
     7141 accccacaca ggctgccgaa cagccctgcc agcaatccat accagacaaa gatggcgcga
     7201 attaaaccat ctttcgcccc cagcgttctt aatactgcga tatcgccact cttgtctttc
     7261 accgccatca ctaaggtgga gacgatgttg aaacaggcca cgccaatcac cagtaccatc
     7321 gccagataca taatggcgcg gatcatctgg atatcgcgat acatatagcc gtaagtacca
     7381 atccaacttt taatataaac atagctgttg gtcacttcac ccgcatcgcg taccagctta
     7441 ttggcgttga aaacatccgt cattttaagg gcaatacctg acacgctgga acccatatca
     7501 agatattgtt gggcatccgc cagcgggatc atggcaaaac tgtgatcgag ttgaccactc
     7561 aactgcaaaa taccggcaac gtgcaaacgc acacgttttg gctgcatcag tttatgctcg
     7621 ggattcgagt tggggatcat aatcgacacc caatccccct gcttcacttt cagcgcatcc
     7681 gccacgcctt tgccgatgat aatttgctgt tcgcccgctt taaaattgcg ccacgcatcg
     7741 ccctggacaa acgagggtaa tgcgctcaga cgctgttcct gttgcgggtt aacgcccttc
     7801 acctggattg cgcgaagatt cgcgccactt tccaccagcc cggtgaaatt gatatacggc
     7861 gcggcagcgg caatacccgg cactttttgc acgtgatcca gtgcttcctg ccagttagtc
     7921 cacggctgat ccaccgcctc gatttcacca tgcggcacca ccgccagaat acggttgttc
     7981 agttcgcgtt caaagccgtt catcgcgctt aagccgacga tcaataccgc cacgccgagg
     8041 gcaatgccaa tggtagaaat aacggagatc agcgacacca tgccgccgcg ccgccgtccg
     8101 cgactaaaac gcaggccaat taataacgat aaaggcatcg ccattactcc gcccccatca
     8161 ggctcagttc cgccgtcaga cgaccatcac gcatttctaa ctggcggctc atacgtttcg
     8221 ccagttgcag gtcgtgagta accaccagga aggcggtgcc ctgcaagcga ttcaattccc
     8281 caagcaactg gaagatgctg tcggcgttac gcgcatcgag gttaccggta ggttcatccg
     8341 ccagtaccag gcgcgggtta ttcaccagcg cacgggcaat cgccacacgc tggcgttcgc
     8401 cgccagaaag ttcagatggg cggtgattcg cacgatgatc cagccccacc gcttttaaca
     8461 tctcaagtgc acggctgttg atttcagcgg gctttttctt gccaatcagc agcggcatag
     8521 ccacgttttc cagggcggta aaatccggca gcaggtggtg aaactgataa ataaagccca
     8581 gcttctggtt gcgcagctct gctttcgccg ccgaagacag tttgctcatc ggttgaccgt
     8641 taaagatcac atcgccggag gttggcgtat ccagcccgcc caacaagtgc agcaaggtgc
     8701 ttttaccgga accagagcta ccgacgatcg ccatcatttc accttcgccg acgctgaaac
     8761 tgacattgtg cagcacatcg gtttgcacac tgccttcctg atagcgtttg cacaggttgt
     8821 cgcattgcaa caggatctta ttcataacgt aaagcctcag cgggttgagt ggcggcagcg
     8881 cgccatgaag ggtaaagcgt agacagcagc gcgatagcca tcgccaccag cgcaataaca
     8941 atgacctgta aaggttcgat agcgaccggc agcgccgcgc catcaagcag gacgccgatt
     9001 atcggcatca gattatttaa ctggctggca agcagtgcgc caagcgccgc accgaggatc
     9061 gcaccgataa tcccggcgct ggccccttgc accataaaga ccatcatgat ttgtcgcgga
     9121 gttaagcctt gcgtttgcag gatcgcgact tcgccctgct tctccattac catcagccct
     9181 agtgaggtaa taatgttaaa cgccgcaacg gcgacaatca ggctcagcag caagcccatc
     9241 atattttttt ccatgcgtac ggcctggaac agctcgcctt tacggtcacg ccagtcctgc
     9301 catttgctgc cttcaggcag tttttgctga cttaaagagt caactttcag cggctcatcc
     9361 agccacaaac gccagccggt aatattgcct gccggataac gcatcagacg cgaggcatcc
     9421 tcaatattca ccagcatttc atagccatcg acttcactgt tggcggcgaa agtgccaatc
     9481 acattgaaca ggcgctggct tggaatacgc cccatcggcg tgaactggct ggcagatggt
     9541 accatcacgc ggatttgatc accgcgatta acgcctagct gtgaggcaag ttgttcgccg
     9601 aggatgacat tatatttccc cggctcgagg tcagtttgtt tcacattgac cagatacggt
     9661 gtaagtggat ctttttgcgc cgggtcgata ccgagcatca ccccgaccgc cacgctgcgc
     9721 gcgctttgca gtaccacatc accggtagta ataggtgcga cgcgattaac gccgtccagt
     9781 ttgactgccg tttccgggag ttgctgcggg ttaagagagc catgctcaga agagagaatt
     9841 gcctgtggca tcaggccaag gatgttgttt tgcagctcgc gctcaaagcc gttcatcact
     9901 gacaatactg tgaccagcgc catcaccccg agggtaatgc cgatggtaga aagccaggag
     9961 acgaaacgac cgaagcgatc cgctgcacgc ccacgcatgt aacgcaggcc aataaataga
    10021 gcgacaggtt ggtacatgaa atccgtctgg ttgctgtagc aaagacacga gtatataagc
    10081 gaaagcaaga tgggtaaacg tctgaactgt aagtgatcgt cagggctaat ctgaaaagcc
    10141 cccgtaaatc aatcggctaa attgccatgt tttatcatca atcttgccgc ctgatcttaa
    10201 tctgaaaaat catccaatac agacagatcc ccattacacc tcgggtcata acccgcagga
    10261 tcaaccgcat gtaaattaaa aaatagcgtg ttgtcctgat gaaacaaaaa gagctatgga
    10321 ttaaccagat caaagggtta tgtatttgtc tggtggtgat ttatcactcg gtcattacct
    10381 tttatccgca tctgaccact tttcagcatc cgttatcgga agtcctgagc aaatgctgga
    10441 tctatttcaa tctttacctt gccccctttc gtatgccggt ttttttcttt atatccggct
    10501 atttgattcg ccgctatatc gacagcgtac cgtggggaaa ttgtctcgat aaacggatct
    10561 ggaacatctt ctgggtgctg gcgctttggg gcgtggtgca gtggctggcg ttaagtgcgc
    10621 tgaaccagtg gctggcacct gagcgcgatt taagcaatgc ctccaatgcc gcttatgccg
    10681 attccaccgg tgagttcctg cacgggatga tcaccgccag caccagcttg tggtatctgt
    10741 atgcgttaat tgtctatttc gtggtatgta aaatttttag ccgtctggcg ctgccactat
    10801 tcgccttgtt tgtactgctg agtgtggcgg ttaatttcgt tcccacgccg tggtggggaa
    10861 tgaacagtgt gatccgcaat ttgctttatt acagccttgg cgcatggttt ggcgcaacaa
    10921 taatgacctg tgttaaagag gtgccgttgc gccgccatct gctgatggct tctttgctta
    10981 ccgttctggc ggtcggtgcc tggttgttta ctatctcgct gctgttgtcg ctggtatcga
    11041 ttgtggtgat catgaagctg ttttatcaat acgagcaacg attcggtatg cgctccacca
    11101 gcctgctgaa tgtgattggc tccaacacca ttgctatcta caccacccat cgcattctgg
    11161 ttgaaatatt cagcttaact ctgcttgcgc aaatgaacgc agcacgctgg tcgccgcaag
    11221 tcgaactgac actcctgctg gtttatccct ttgtcagttt gttcatctgt actgttgcgg
    11281 gcttgctggt aagaaaactt tcacagcgtg cattcagcga tctgttgttc tccccgcctt
    11341 cactgcccgc tgccgtcagt tactctcgtt aaaccatcgc cccagccggg gcgattcaat
    11401 ttgctaaacc atgtcgatta cggataatga acagcattgc ttatcagaga tatgccccca
    11461 tatgttgagg catatcctaa cgagaatctg acaaccgtta tgcctgaaca atatcgttat
    11521 acgctgcccg tcaaagcggg tgagcagcgt ctgctgggcg agttaaccgg cgcagcctgt
    11581 gcaacgctgg tagcggaaat tgccgaacgt cacgccggtc cggtggtact cattgcacca
    11641 gatatgcaaa atgctctgcg tttgcatgat gaaatcagcc agttcaccga tcagatggtg
    11701 atgaatctgg cggactggga aactcttccc tacgacagtt tttcgcctca tcaggacatt
    11761 atctcctcgc gcctttccac cctttaccag ctaccgacga tgcagcgtgg cgtactgatt
    11821 gttccggtga atacgcttat gcagcgcgtt tgcccacaca gttttctcca cggtcatgcg
    11881 ctggtgatga aaaaaggtca gcgcctgtca cgagatgcat tacgaaccca actggacagc
    11941 gccggttatc gccatgttga ccaggtgatg gagcacggcg aatacgccac gcgcggcgcg
    12001 ttgctggatc tcttcccgat ggggagtgag ctgccttatc gtcttgattt ctttgatgat
    12061 gaaatcgaca gcctgcgggt gtttgacgtc gacagccagc gcacgctgga ggaagtagaa
    12121 gcgatcaatc tgctgcccgc gcacgaattt ccgaccgata aagcggcaat tgaactgttc
    12181 cgcagccagt ggcgcgatac cttcgaagtg aagcgcgatc cggaacatat ttaccagcaa
    12241 gtgagtaaag gcacattacc tgccgggatc gagtactggc agccattgtt cttcagcgaa
    12301 ccactgccgc cgctgttcag ttatttccct gccaatacct tgctcgtgaa tactggcgat
    12361 ctggaaacca gtgccgaacg tttccaggct gacacgctgg cgcgttttga gaatcgcggc
    12421 gtcgatccga tgcgcccgct gttgccacca caatcgctct ggctgcgggt ggacgagctc
    12481 ttctcagagc tgaaaaactg gccccgggtg cagctaaaaa ctgaacattt accgacaaaa
    12541 gccgcgaatg ccaatttagg tttccagaaa ctgccagacc tggccgttca ggcacaacaa
    12601 aaagcgccgc tggatgcgct gcgtaagttc ctcgagactt tcgacggtcc ggtggtgttc
    12661 tcggtagaaa gtgaaggtcg ccgtgaagcg ctgggtgaac tgctcgcacg aattaaaatt
    12721 gctccgcaac gcattatgcg tcttgatgaa gccagcgacc gtgggcgtta tctgatgatt
    12781 ggcgctgccg aacatggttt tgtcgataag gtgcgtaatc tggcgctgat ctgcgaaagc
    12841 gatctgctcg gtgaacgtgt tgcccgccgt cgtcaggatt ctcgccgcac catcaacccc
    12901 gatacactga tccgtaacct tgcggagctg catattggtc agccggtggt ccatctggag
    12961 cacggtgtcg ggcgctatgc cggaatgacc acgctcgaag cgggcggcat tactggcgag
    13021 tatttgatgc tcacctatgc caacgacgcc aaactgtatg ttccggtgtc gtcactgcat
    13081 ctgattagcc gttacgcagg tggcgcggaa gaaaacgccc cgctgcataa acttggcggc
    13141 gatgcgtggt cacgcgcgcg gcagaaagcg gcggaaaaag tgcgtgatgt ggcggcggaa
    13201 ttgctggata tctacgcgca acgcgccgcc aaagagggct tcgcgtttaa acacgatcgt
    13261 gagcagtatc agttgttctg cgacagcttc ccgtttgaaa ccacgccgga tcaggcgcag
    13321 gccattaatg cggtacttag cgacatgtgt cagccgctgg caatggatcg tctggtgtgc
    13381 ggcgatgttg gctttggtaa aacagaagtg gcgatgcgcg ccgctttcct ggcagtagat
    13441 aaccacaagc aggtggcggt gttggtgcct accacccttc tcgcgcagca gcattacgac
    13501 aacttccgcg accgtttcgc caactggccg gtacgtatcg aaatgatctc ccgtttccgc
    13561 agcgccaaag agcagacgca aatccttgcg gaagtggcgg aagggaaaat cgatattctg
    13621 atcggtacgc acaaactgct gcaaagtgac gtcaagttta aagatttagg cctgctgatt
    13681 gtcgatgaag aacaccgctt cggggtgcgt cataaagagc gtattaaagc gatgcgcgcg
    13741 aacgtggata ttctgacgct tactgcaacg ccgatcccac gtacgctgaa tatggcaatg
    13801 agcggaatgc gtgacctgtc gattatcgcc acgccgcccg cccgtcgtct ggcagttaaa
    13861 acctttgtcc gtgagtatga cagcctggtg gtccgggagg cgatcctgcg tgaaattttg
    13921 cgcggaggac aggtttatta tctctacaat gatgtggaaa acattcagaa agccgccgaa
    13981 cggctggcag aactggtgcc agaagcgcgg atcgccatcg gtcacgggca gatgcgcgag
    14041 cgcgaactgg aacgggtgat gaatgatttc catcatcaac gtttcaacgt gctggtttgt
    14101 acaaccatta tcgaaaccgg gatcgacatc ccgacagcca acaccattat cattgaacgc
    14161 gcggatcact tcggtctggc gcagctgcac cagttacgcg gtcgcgtcgg acgttcacat
    14221 catcaggcat atgcatggtt gctgacgccg catccaaaag cgatgactac cgatgcacaa
    14281 aaacgtcttg aagcgattgc ctcgctggaa gatctcgggg caggttttgc gctggcaacg
    14341 cacgatctgg agattcgcgg cgcgggtgaa ctgcttggcg aagaacaaag cggctcaatg
    14401 gaaaccatcg gtttctcgct gtatatggag ttgctggaaa acgccgtcga tgcactgaaa
    14461 gccggacgcg agccgtcgct ggaagatctc accagccagc aaacagaagt cgagctgcgg
    14521 atgccgtcgc tattgccaga tgatttcatt cctgacgtga atacgcgttt gtcgttctat
    14581 aaacgtattg ccagcgccaa aacggaaaac gaactggaag agatcaaagt cgagcttatc
    14641 gatcgcttcg gcctgctgcc ggatccggcg cgtaccctgc tggatgttgc ccgtctgcgc
    14701 cagcaagcgc agaaactggg gatcaggaag ctggaaggta atgagaaagg cggggtgatc
    14761 gaatttgccg agaagaatca cgttaatccg gcctggttga ttggtttgct gcaaaaacag
    14821 ccgcagcatt accgccttga tggtccgacg cgcctgaaat ttattcagga tttgagtgag
    14881 cggaaaacgc gtatcgaatg ggtacgccag tttatgcgtg aactggaaga gaacgcgatc
    14941 gcttaatggt aaatcggggg cgtttctgcg cccccatctg caacatttac aaattctttg
    15001 cacttccctg cactatccga cactgtcacc atccataatt caggcttatc tgtttattac
    15061 aataacctta tatttattat ggatttttgg tgatgatcaa aacgcgtttt tctcgctggc
    15121 taacgttttt tacgttcgcc gctgccgtgg cgctggcgct accggcaaaa gccaacacct
    15181 ggccgctgcc gccagcgggc agtcgtctgg ttggcgaaaa caaatttcat gtggtggaaa
    15241 atgacggtgg ttctctggaa gccatcgcca aaaaatataa cgtcggcttt ctcgctctgt
    15301 tacaggctaa ccctggcgtt gatccttacg taccgcgcgc gggtagcgtg ttaacgatcc
    15361 cgttgcaaac cctacttcca gatgcgccgc gcgaaggcat tgtgatcaat attgcggagc
    15421 tgcgtctcta ttactacccg ccgggtaaaa attcggtaac cgtgtatcca ataggtattg
    15481 gtcagttagg tggtgacacg ctgacaccga caatggtgac caccgtttca gacaaacgtg
    15541 caaacccaac ctggacgcca acggcaaaca tccgcgcccg ttataaagca cagggaattg
    15601 agttgcctgc ggtagtgccg gctggaccgg ataacccaat gggccatcat gcgattcgtc
    15661 tggcggccta tggcggcgtt tatttgcttc acggtacgaa cgccgatttc ggcattggca
    15721 tgcgggtaag ttctggctgt attcgtctgc gggatgacga tatcaaaaca ctctttagcc
    15781 aggtcacccc aggcaccaaa gtgaatatca tcaacactcc gataaaagtc tctgccgaac
    15841 caaacggtgc gcgtctggtt gaagtacatc agccgctgtc agagaagatt gatgacgatc
    15901 cgcagctgct gccaattacg ctgaatagcg caatgcaatc atttaaagat gcagcacaaa
    15961 ctgacgctga agtgatgcaa catgtgatgg atgtccgttc cgggatgccg gtggatgtcc
    16021 gccgtcatca agtgagccca caaactctgt aaatcctgga taaaaaaaat ccccgcaaca
    16081 cggcatgtcg cggggaaaaa atcagcagta gcaggcatta atgagggtta atgcttattt
    16141 ataaattact gctgttccat ggagggtatt cggaccggtt acagaagtaa tacggaaaga
    16201 ttttgcgccc atctcatccg ctttttgcgc cagctgctct tccagcgatc ccagatttgt
    16261 ccccgcgtta gcactgattg taccgacttt ttgttggcct tctggcgttg actgaacttc
    16321 gacagccgca aagctggcaa atgacatgga gcttaaaatc gccgcagcga tgagggtttt
    16381 tacgtttttc ataatagtgg ccttatgcag atgaatgacg tcgcaagcaa ttacttaacg
    16441 atcgataact aaatgataga tgtgatctgg atcacataca agatattttt tataacaatc
    16501 attaattaat t
//
LOCUS       DAZKHF020000083        16351 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2201S31241
            SAMN43267800-rid25106413.denovo.083, whole genome shotgun sequence.
ACCESSION   DAZKHF020000083 DAZKHF020000000
VERSION     DAZKHF020000083.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267800
            Sequence Read Archive: SRR30307263
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 16351)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16351)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 16351)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350581.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350581.2
            Genome Coverage       :: 151x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:21:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,652
            CDSs (total)                      :: 5,557
            Genes (coding)                    :: 5,320
            CDSs (with protein)               :: 5,320
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 237
            CDSs (without protein)            :: 237
            Pseudo Genes (ambiguous residues) :: 0 of 237
            Pseudo Genes (frameshifted)       :: 102 of 237
            Pseudo Genes (incomplete)         :: 130 of 237
            Pseudo Genes (internal stop)      :: 52 of 237
            Pseudo Genes (multiple problems)  :: 41 of 237
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16351
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267800-rid25106413.denovo.083"
                     /serotype="O 26:H11"
                     /isolate="2201S31241"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-01"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            complement(86..2047)
                     /gene="topB"
                     /locus_tag="ACHOSC_RS11325"
                     /old_locus_tag="pgaptmp_002265"
     CDS             complement(86..2047)
                     /gene="topB"
                     /locus_tag="ACHOSC_RS11325"
                     /old_locus_tag="pgaptmp_002265"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase III"
                     /protein_id="HGU4548195.1"
                     /translation="MRLFIAEKPSLARAIADVLPKPHRKGDGFIECGNGQVVTWCIGH
                     LLEQAQPDAYDSRYARWNLADLPIVPEKWQLQPRPSVTKQLNVIKRFLHEASEIVHAG
                     DPDREGQLLVDEVLDYLQLAPEKRQQVQRCLINDLNPQAVERAIDRLRSNSEFVPLCV
                     SALARARADWLYGINMTRAYTILGRNAGYQGVLSVGRVQTPVLGLVVRRDEEIENFVA
                     KDFFEVKAHIVTPADERFTAIWQPSEACEPYQDEEGRLLHRPLAEHVVNRISGQPAIV
                     TSYNDKRESESAPLPFSLSALQIEAAKRFGLSAQNVLDICQKLYETHKLITYPRSDCR
                     YLPEEHFAGRHAVMNAISVHAPDLLPQSVVDPDIRNRCWDDKKVDAHHAIIPTARSSA
                     INLTENEAKVYNLIARQYLMQFCPDAVFRKCVIELDIAKGKFVAKARFLAEAGWRTLL
                     GSKERDEENDGMPLPVVAKGDELLCEKGEVVERQTQPPRHFTDATLLSAMTGIARFVQ
                     DKDLKKILRATDGLGTEATRAGIIELLFKRGFLTKKGRYIHSTDAGKALFHSLPEMAT
                     RPDMTAHWESVLTQISEKQCRYQDFMQPLVGTLYQLIDQAKRTPVRQFRGIVAPGSGG
                     SADKKKAAPRKRSAKKSPPADEVGSGAIA"
     gene            complement(2052..3095)
                     /gene="selD"
                     /locus_tag="ACHOSC_RS11330"
                     /old_locus_tag="pgaptmp_002266"
     CDS             complement(2052..3095)
                     /gene="selD"
                     /locus_tag="ACHOSC_RS11330"
                     /old_locus_tag="pgaptmp_002266"
                     /EC_number="2.7.9.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001293900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004756 - selenide, water dikinase
                     activity [Evidence IEA];
                     GO_process: GO:0016260 - selenocysteine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="selenide, water dikinase SelD"
                     /protein_id="HGU4548196.1"
                     /translation="MSENSIRLTQYSHGAGCGCKISPKVLETILHSEQAKFVDPNLLV
                     GNETRDDAAVYDLGNGTSVISTTDFFMPIVDNPFDFGRIAATNAISDIFAMGGKPIMA
                     IAILGWPINKLSPEIAREVTEGGRYACRQAGIALAGGHSIDAPEPIFGLAVTGIVPTE
                     RVKKNSTAQAGCKLFLTKPLGIGVLTTAEKKSLLKPEHQGLATEVMCRMNIAGASFAN
                     IEGVKAMTDVTGFGLLGHLSEMCQGAGVQARVDYDAIPKLPGVEEYIKLGAVPGGTER
                     NFASYGHLMGEMPREVRDLLCDPQTSGGLLLAVMPEAENEVKTTAAEFGIELTAIGEL
                     VPARGGRAMVEIH"
     gene            complement(3212..3763)
                     /locus_tag="ACHOSC_RS11335"
                     /old_locus_tag="pgaptmp_002267"
     CDS             complement(3212..3763)
                     /locus_tag="ACHOSC_RS11335"
                     /old_locus_tag="pgaptmp_002267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H nitroreductase"
                     /protein_id="HGU4548197.1"
                     /translation="MDALELLINRRSASRLAEPAPTGEQLQNILRAGMRAPDHKSMQP
                     WHFFVIEGEGRERFSAVLEQGAIAAGSDDKAIDKARNAPFRAPLIITVVAKCEENHKV
                     PRWEQEMSAGCAVMAMQMAAVAQGFGGIWRSGALTESPVVREAFGCREQDKIVGFLYL
                     GTPQLKASTSINVPDPTPFVTYF"
     gene            3924..5780
                     /gene="sppA"
                     /locus_tag="ACHOSC_RS11340"
                     /old_locus_tag="pgaptmp_002268"
     CDS             3924..5780
                     /gene="sppA"
                     /locus_tag="ACHOSC_RS11340"
                     /old_locus_tag="pgaptmp_002268"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707347.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptide peptidase SppA"
                     /protein_id="HGU4548198.1"
                     /translation="MRTLWRFIAGFFKWTWRLLNFVREMVLNLFFIFLVLVGVGIWMQ
                     VSGGDSKETASRGALLLDISGVIVDKPDSSQRFSKLSRQLLGASSDRLQENSLFDIVN
                     TIRQAKDDRNITGIVMDLKNFAGGDQPSMQYIGKALKEFRDSGKPVYAVGENYSQGQY
                     YLASFANKIWLSPQGVVDLHGFATNGLYYKSLLDKLKVSTHVFRVGTYKSAVEPFIRD
                     DMSPAAREADSRWIGELWQNYLNTVAANRQIPAQQVFPGAQGLLEGLTKTGGDTAKYA
                     LENKLVDALASSAEIEKALTKEFGWSKTDKNYRAISYYDYALKTPADTGDSIGVVFAN
                     GAIMDGEETQGNVGGGTTAAQIRDARLDPKVKAIVLRVNSPGGSVTASEVIRAELAAA
                     RAAGKPVVVSMGGMAASGGYWISTPANYIVANPSTLTGSIGIFGVITTVENSLDSIGV
                     HTDGVSTSPLADVSITRALPPEAQQMMQLSIENGYKRFITLVADARHSTPEQIDKIAQ
                     GHVWTGQDAKANGLVDSLGDFDDAVAKAAELAKVKQWHLEYYVDEPTFFDKVMDNMSG
                     SVRAMLPDAFQAMLPAPLASVASTVKSESDKLAAFNDPQNRYAFCLTCANVR"
     gene            5947..6963
                     /gene="ansA"
                     /locus_tag="ACHOSC_RS11345"
                     /old_locus_tag="pgaptmp_002269"
     CDS             5947..6963
                     /gene="ansA"
                     /locus_tag="ACHOSC_RS11345"
                     /old_locus_tag="pgaptmp_002269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004067 - asparaginase activity [Evidence
                     IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="asparaginase"
                     /protein_id="HGU4548199.1"
                     /translation="MQKKSIYVAYTGGTIGMQRSEQGYIPVSGHLQRQLALMPEFHRP
                     EMPDFTIHEYTPLMDSSDMTPEDWQHIAEDIKAHYDDYDGFVILHGTDTMAYTASALS
                     FMLENLGKPVIVTGSQIPLAELRSDGQINLLNALYVAANYPINEVTLFFNNRLYRGNR
                     TTKAHADGFDAFASPNLPPLLEAGIHIRRLNTPPAPHGEGELIVHPITPQPIGVVTIY
                     PGISADVVRNFLRQPVKALILRSYGVGNAPQNKAFLQELQEASDRGIVVVNLTQCMSG
                     KVNMGGYATGNALAHAGVIGGADMTVEATLTKLHYLLSQELDTETIRKAMSQNLRGEL
                     TPDD"
     gene            6974..7615
                     /gene="pncA"
                     /locus_tag="ACHOSC_RS11350"
                     /old_locus_tag="pgaptmp_002270"
     CDS             6974..7615
                     /gene="pncA"
                     /locus_tag="ACHOSC_RS11350"
                     /old_locus_tag="pgaptmp_002270"
                     /EC_number="3.5.1.-"
                     /EC_number="3.5.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310502.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional nicotinamidase/pyrazinamidase"
                     /protein_id="HGU4548200.1"
                     /translation="MPHRALLLVDLQNDFCAGGALAVPEGDSTVDVANRLIDWCQSRG
                     EAVIASQDWHPANHGSFASQHGVEPYTPGQLDGLPQTFWPDHCVQNSEGAQLHPLLNQ
                     KAIAAVFHKGENPLVDSYSAFFDNGRRQKTSLDDWLRDHEIDELIVMGLATDYCVKFT
                     VLDALQLGYKVNVITDGCRGVNIQPQDSAHAFMEMSAAGATLYTLADWEETQG"
     gene            complement(7776..8048)
                     /locus_tag="ACHOSC_RS11355"
                     /old_locus_tag="pgaptmp_002271"
     CDS             complement(7776..8048)
                     /locus_tag="ACHOSC_RS11355"
                     /old_locus_tag="pgaptmp_002271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707337.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YeaC family protein"
                     /protein_id="HGU4548201.1"
                     /translation="MNLDDIINSMTPEVYQRLSTAVELGKWPDGVALTEEQKENCLQL
                     VMLWQARHNTEAQHMTIDTNGQMVMKSKQQLKEDFGISAKPIAMFK"
     gene            complement(8090..8503)
                     /gene="msrB"
                     /locus_tag="ACHOSC_RS11360"
                     /old_locus_tag="pgaptmp_002272"
     CDS             complement(8090..8503)
                     /gene="msrB"
                     /locus_tag="ACHOSC_RS11360"
                     /old_locus_tag="pgaptmp_002272"
                     /EC_number="1.8.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033743 - peptide-methionine (R)-S-oxide
                     reductase activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (R)-S-oxide reductase MsrB"
                     /protein_id="HGU4548202.1"
                     /translation="MANKPSAEELKKNLSEMQFYVTQNHGTEPPFTGRLLHNKRDGVY
                     HCLICDAPLFHSETKYDSGCGWPSFYEPVSEESIRYIKDLSHGMQRIEIRCGNCDAHL
                     GHVFPDGPQPTGERYCVNSASLRFTDGENGEEING"
     gene            8845..9840
                     /gene="gapA"
                     /locus_tag="ACHOSC_RS11365"
                     /old_locus_tag="pgaptmp_002273"
     CDS             8845..9840
                     /gene="gapA"
                     /locus_tag="ACHOSC_RS11365"
                     /old_locus_tag="pgaptmp_002273"
                     /EC_number="1.2.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006322611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HGU4548203.1"
                     /translation="MTIKVGINGFGRIGRIVFRAAQKRSDIEIVAINDLLDADYMAYM
                     LKYDSTHGRFDGTVEVKDGHLIVNGKKIRVTAERDPANLKWDEVGVDVVAEATGLFLT
                     DETARKHITAGAKKVVMTGPSKDNTPMFVKGANFDKYAGQDIVSNASCTTNCLAPLAK
                     VINDNFGIIEGLMTTVHATTATQKTVDGPSHKDWRGGRGASQNIIPSSTGAAKAVGKV
                     LPELNGKLTGMAFRVPTPNVSVVDLTVRLEKAATYEQIKAAVKAAAEGEMKGVLGYTE
                     DDVVSTDFNGEVCTSVFDAKAGIALNDNFVKLVSWYDNETGYSNKVLDLIAHISK"
     gene            9924..10808
                     /locus_tag="ACHOSC_RS11370"
                     /old_locus_tag="pgaptmp_002274"
     CDS             9924..10808
                     /locus_tag="ACHOSC_RS11370"
                     /old_locus_tag="pgaptmp_002274"
                     /EC_number="5.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707334.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016857 - racemase and epimerase activity,
                     acting on carbohydrates and derivatives [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-hexose-6-phosphate mutarotase"
                     /protein_id="HGU4548204.1"
                     /translation="MIKKIFALPVIEQISPVLSRRKLDELDLIVVDHPQVKASFALQG
                     AHLLSWKPAGEEEVLWLSNNTPFKNGVAIRGGVPVCWPWFGPAAQQGLPAHGFARNLP
                     WTLKSHREDADGVALTFELTQSEETKKFWPHDFTLLAHFRVGKTCEIDLESHGEFETT
                     SALHTYFNVGDIAKVSVSGLGDRFIDKVNDAKEDVLTDGIQTFPDRTDRVYLNPQDCS
                     VINDEALNRIIAVGHQHHLNVVGWNPGPALSVSMGDMPDDGYKTFVCVETAYASETQK
                     VTKEKPAHLAQSIRVAKR"
     gene            complement(10859..11713)
                     /gene="yeaE"
                     /locus_tag="ACHOSC_RS11375"
                     /old_locus_tag="pgaptmp_002275"
     CDS             complement(10859..11713)
                     /gene="yeaE"
                     /locus_tag="ACHOSC_RS11375"
                     /old_locus_tag="pgaptmp_002275"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylglyoxal reductase YeaE"
                     /protein_id="HGU4548205.1"
                     /translation="MQQKMIQFSGDVSLPAIGQGTWYMGEDASQRKTEVAALRAGIEL
                     GLTLIDTAEMYADGGAEKVVGEALTGLRDNVFLVSKVYPWNAGGQKAINACEASLRRL
                     NTDYLDLYLLHWSGSFAFEETVAAMEKLIAQGKIRRWGVSNLDHADMQELWQLPGGNQ
                     CATNQVLYHLGSRGIEYDLLPWCQQQQMPVMAYSPLAQAGRLRNGLLKNAVVNEIAHA
                     HNISAAQVLLAWVISHQGVMAIPKAATIAHVQQNAAVLEVELSSAELTMLDKAYPAPK
                     GKTALDMV"
     gene            complement(11803..12549)
                     /gene="mipA"
                     /locus_tag="ACHOSC_RS11380"
                     /old_locus_tag="pgaptmp_002276"
     CDS             complement(11803..12549)
                     /gene="mipA"
                     /locus_tag="ACHOSC_RS11380"
                     /old_locus_tag="pgaptmp_002276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="scaffolding protein MipA"
                     /protein_id="HGU4548206.1"
                     /translation="MTKLKLLALGVLIATSAGVAHAEGKFSLGAGVGVVEHPYKDYDT
                     DVYPVPVINYEGDNFWFRGLGGGYYLWNDATDKLSITAYWSPLYFKAKDSGDHQMRHL
                     DDRKSTMMAGLSYAHFTQYGYLRTTLAGDTLDNSNGIVWDMAWLYRYTNGGLTVTPGI
                     GVQWNSENQNEYYYGVSRKESARSGLRGYNPNDSWSPYLELSASYNFLGDWSVYGTAR
                     YTRLSDEVTDSPMVDKSWTGLISTGITYKF"
     gene            12985..14919
                     /gene="yeaG"
                     /locus_tag="ACHOSC_RS11385"
                     /old_locus_tag="pgaptmp_002277"
     CDS             12985..14919
                     /gene="yeaG"
                     /locus_tag="ACHOSC_RS11385"
                     /old_locus_tag="pgaptmp_002277"
                     /EC_number="2.7.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011411173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase YeaG"
                     /protein_id="HGU4548207.1"
                     /translation="MNIFDHYRQRYEAAKDEEFTLQEFLTTCRQDRSAYANAAERLLM
                     AIGEPVMVDTAQEPRLSRLFSNRVIARYPAFEEFYGMEDAIEQIVSYLKHAAQGLEEK
                     KQILYLLGPVGGGKSSLAERLKSLMQLVPIYVLSANGERSPVNDHPFCLFNPQEDAQI
                     LEKEYGIPRRYLGTIMSPWAAKRLHEFGGDITKFRVVKVWPSILQQIAIAKTEPGDEN
                     NQDISALVGKVDIRKLEHYAQNDPDAYGYSGALCRANQGIMEFVEMFKAPIKVLHPLL
                     TATQEGNYNGTEGISALPFNGIILAHSNESEWVTFRNNKNNEAFLDRVYIVKVPYCLR
                     ISEEIKIYEKLLNHSELTHAPCAPGTLETLSRFSILSRLKEPENSSIYSKMRVYDGES
                     LKDTDPKAKSYQEYRDYAGVDEGMNGLSTRFAFKILSRVFNFDHVEVAANPVHLFYVL
                     EQQIEREQFPQEQAERYLEFLKGYLIPKYAEFIGKEIQTAYLESYSEYGQNIFDRYVT
                     YADFWIQDQEYRDPDTGQLFDRESLNAELEKIEKPAGISNPKDFRNEIVNFVLRARAN
                     NSGRNPNWTSYEKLRTVIEKKMFSNTEELLPVISFNAKTSTDEQKKHDDFVDRMMEKG
                     YTRKQVRLLCEWYLRVRKSS"
     gene            15032..16315
                     /locus_tag="ACHOSC_RS11390"
                     /old_locus_tag="pgaptmp_002278"
     CDS             15032..16315
                     /locus_tag="ACHOSC_RS11390"
                     /old_locus_tag="pgaptmp_002278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YeaH/YhbH family protein"
                     /protein_id="HGU4548208.1"
                     /translation="MTWFIDRRLNGKNKSMVNRQRFLRRYKAQIKQSISEAINKRSVT
                     DVDSGESVSIPTEDISEPMFHQGRGGLRHRVHPGNDHFVQNDRIERPQGGGGGSGSGQ
                     GQASQDGEGQDEFVFQISKDEYLDLLFEDLALPNLKQNQQRQLTEYKTHRAGYTANGV
                     PANISVVRSLQNSLARRTAMTAGKRRELHALEENLAIISNSEPAQLLEEERLRKEIAE
                     LRAKIERVPFIDTFDLRYKNYEKRPDPSSQAVMFCLMDVSGSMDQSTKDMAKRFYILL
                     YLFLSRTYKNVEVVYIRHHTQAKEVDEHEFFYSQETGGTIVSSALKLMDEVVKERYNP
                     AQWNIYAAQASDGDNWADDSPLCHEILAKKLLPVVRYYSYIEITRRAHQTLWREYEHL
                     QSTFDNFAMQHIRDQDDIYPVFRELFHKQNATAKD"
BASE COUNT         4012 a         4241 c         4057 g         4041 t
ORIGIN      
        1 ccatgcaaaa aaaatatcca acgaattttc ttgatccgct ggatatttca gaatatgact
       61 cgaatagcac gaaagattca ctcgcttacg ctatcgcccc gcttccgact tcatctgctg
      121 gcggactttt tttcgcacta cgtttacgcg gtgcagcctt tttcttatca gcactgccac
      181 cactgcccgg agccacaatg ccgcgaaact gccgcaccgg cgtacgtttg gcttgatcaa
      241 taagctgata tagcgtcccc accagcggct gcataaagtc ctgatagcga cactgctttt
      301 cgctgatttg cgtcagcacc gattcccagt gcgcggtcat gtccggtcgc gtcgccattt
      361 ctggcagcga atggaataac gcttttccgg cgtcggtaga gtggatatag cgcccttttt
      421 tggtcagaaa accacgcttg aacagcagtt caataatccc ggcacgggtt gcttctgtcc
      481 ccagaccatc ggtcgcacga aggatctttt tcagatcttt gtcctgcaca aagcgcgcga
      541 tcccggtcat cgccgaaagc agtgttgcat cggtaaaatg gcgcggcggc tgggtttgcc
      601 gctctaccac ttcacctttt tcacacagca actcatcgcc tttcgccacc acaggcaatg
      661 gcataccgtc gttttcttca tcgcgctctt tgctgcctaa cagcgtgcgc cagcctgctt
      721 cagcaagaaa acgggcttta gcgacaaatt tgcctttggc aatgtccagt tcgataacac
      781 acttgcggaa caccgcatcc gggcagaact gcatcaggta ttgacgggca atcaggttat
      841 agaccttcgc ttcgttctcc gtcaggttga tcgcagaact ccgtgcggtc gggatgatgg
      901 cgtggtgcgc atcgaccttt ttgtcatccc aacagcggtt gcgtatatct ggatctacca
      961 ctgactgcgg caacagatcc ggtgcatgaa cgctgatggc attcatcacc gcgtggcgtc
     1021 cggcaaaatg ttcttctggc aaatagcgac aatcggaacg cggataagtg attagcttgt
     1081 gcgtttcgta cagcttctgg cagatatcaa gcacgttctg cgcgctcaga ccgaagcgtt
     1141 ttgccgcttc aatctgtaac gccgaaagcg aaaacggcag cggcgcggat tctgattccc
     1201 gtttatcgtt atagctggtg acaatagccg gttgaccact aatgcggtta accacatgct
     1261 ccgccagtgg acgatgtaac aagcgccctt cttcatcctg gtacggttca cacgcttcgc
     1321 tcggttgcca gatagcggta aaccgctcat cggcaggtgt cacgatatgc gctttgactt
     1381 caaagaagtc tttcgccacg aagttttcaa tctcttcatc gcggcgcacc accagcccga
     1441 gcacgggtgt ctgcacgcgt cccacggaaa gtacgccctg ataaccggca ttgcgaccga
     1501 gaatggtata cgcacgggtc atattgatgc cgtacagcca gtcggcacgc gctcgcgcca
     1561 gcgcagaaac gcacagcggt acaaactcac tgttggaacg aagacggtcg atcgcccgct
     1621 caaccgcctg cgggttcagg tcgtttatca agcaacgctg cacctgctgg cgcttttccg
     1681 gtgccagttg cagatagtcc agcacttcat ccaccagcaa ttgcccttca cgatccgggt
     1741 ccccggcgtg aacgatttcg ctggcttcat gcaggaagcg tttgatgacg ttaagttgtt
     1801 tggtcacgga gggtcggggc tgtaattgcc acttttccgg gacaatcggc aaatccgcaa
     1861 gattccagcg cgcatagcgg ctgtcgtagg cgtctggctg cgcctgctca agcaggtgac
     1921 cgatacacca ggtcaccacc tgaccattac cgcactcgat aaagccatcg cctttacggt
     1981 gcggtttggg caggacatca gcaatggcgc gcgccagact cggtttttcg gcaataaaca
     2041 accgcattga attaatggat ctcaaccatg gcacgaccgc cgcgcgctgg caccagttcg
     2101 ccaatcgccg tcagttcaat gccaaactcg gcggctgtag ttttaacctc attttctgct
     2161 tccggcatga ccgccagcag caaaccgcca gaagtttgcg gatcgcacag cagatcgcgc
     2221 acttcacgcg gcatttcacc catcagatga ccgtagctgg caaagttacg ttcggtgccg
     2281 ccaggcactg cgcccaactt aatgtactct tcaacccccg gcaattttgg gatcgcgtca
     2341 tagtcaacgc gcgcctgcac acctgcgccc tgacacattt cgctcaaatg gcccagcaga
     2401 ccaaaaccag taacgtcggt catcgctttt acgccttcga tgttggcaaa ggacgcacct
     2461 gcgatgttca tccggcacat cacttccgtc gccagtccct gatgttctgg tttcaacagt
     2521 gattttttct cagccgtggt aagaacgccg atccccagcg gtttcgtcag gaacagtttg
     2581 catccggctt gtgcggtact gtttttcttc acccgctcgg tcggtacgat ccccgttacc
     2641 gccagaccaa aaatcggctc cggcgcatcg atggagtgac cgccagccag cgcaataccc
     2701 gcctgacggc atgcatagcg tccaccttcg gttacttcgc gggcaatttc tggggaaagt
     2761 ttgttaatcg gccagccgag gatcgcaatc gccataatcg gtttgccccc catcgcgaag
     2821 atatcgctga tggcgttagt cgccgcaatg cggccaaaat cgaaaggatt atcaacgatc
     2881 ggcataaaga agtcggtggt actgataacg ctggtgccat tgcccagatc gtacaccgcc
     2941 gcatcgtcgc gggtctcatt acccacaagc aaattcggat caacaaactt cgcctgctca
     3001 ctgtgcagga tggtttccaa cacttttggg gaaattttac agccgcaacc agctccgtgg
     3061 ctgtattggg tcaaacgaat cgagttctcg ctcatggaca tctcctgtca atgcaatccg
     3121 ggtatggtaa ccctcattcc gtgaagtgat aagtgagagt gtctgaattc ctgcgccttt
     3181 gctcacaatc cagacagttt cgcgacaatt atcagaaata agttacaaac ggcgtcgggt
     3241 ccgggacgtt aatcgacgta gatgctttca actgcggcgt gccgaggtag agaaaaccga
     3301 caattttatc ctgctcacgg caaccgaatg cttcacgcac taccggactt tcagttaatg
     3361 cgccactgcg ccagatgccg ccaaatcctt gggcaacggc tgccatttgc atcgccatga
     3421 ccgcgcatcc ggcagacatt tcctgttccc atcgcgggac tttatgattt tcttcgcatt
     3481 tcgccaccac cgtgatgatg agcggtgcgc ggaacggcgc attacgggct ttgtcgatag
     3541 ctttgtcatc actaccggca gcaatcgccc cctgttccag tacggcgctg aaacgctcgc
     3601 gcccttcccc ttcaatcaca aaaaaatgcc acggttgcat ggacttatgg tccggcgcac
     3661 gcatacccgc acgcaggatg ttttgcagtt gttcacccgt tggcgcgggt tcagccaggc
     3721 gggaggcgct acggcgattg atcaatagtt cgagtgcatc catttgatta actcctgtcg
     3781 tgatatttat tcacaaaatt aacacgagag tggattttgt tacagcacag tccgcaattc
     3841 ctgctgacaa gtgccggttg ggtcattacg ataaccacat ctattgcgcc tgtgacaggt
     3901 gtgaccttaa gttgggagaa tacatgcgaa ccctttggcg atttattgcc ggatttttta
     3961 aatggacgtg gcgtctgctg aatttcgtcc gtgaaatggt acttaacctg ttctttattt
     4021 tcctcgtact ggttggtgtg gggatctgga tgcaggtcag tggtggtgat tcgaaagaaa
     4081 cggccagtcg tggcgcactg ctgctggaca tttctggtgt gatcgtcgat aaacccgaca
     4141 gttctcagcg gtttagtaaa ttaagccgcc agctgcttgg tgccagttcc gatcgtctgc
     4201 aggaaaactc actgtttgat atcgtcaaca ccattcgcca ggcgaaggac gaccgcaata
     4261 tcaccggtat cgtgatggat ctgaaaaact tcgcaggcgg cgaccagccg tctatgcagt
     4321 acatcggcaa agccctgaaa gagtttcgtg acagcgggaa accggtttat gccgttggcg
     4381 agaactacag ccaggggcaa tattatctcg ccagtttcgc caataaaatt tggctgtcac
     4441 cgcaaggcgt ggttgatctg cacggttttg ctaccaacgg tctgtactac aaatcgttgc
     4501 tggataagct gaaagtttcc acccatgtgt tccgcgtggg tacgtataaa tctgccgttg
     4561 aaccgtttat tcgtgatgat atgtcaccgg cagcccgcga agctgacagc cgctggattg
     4621 gtgagctgtg gcaaaactat ctgaatactg ttgccgctaa ccggcagatc cctgctcagc
     4681 aggtattccc tggcgcgcaa gggttgcttg agggtttaac caaaaccggt ggcgataccg
     4741 cgaaatatgc actggaaaac aagctggtcg atgcactggc atcgagtgcc gaaatcgaaa
     4801 aagctctgac caaagagttc ggctggagta agactgataa aaattatcgc gccatcagtt
     4861 attacgatta cgcattgaaa acgccggcag ataccggtga cagcatcggt gtcgtcttcg
     4921 ctaatggcgc aattatggat ggcgaggaaa ctcaggggaa tgttggcggt ggtaccactg
     4981 cggcacaaat ccgcgacgct cgccttgacc cgaaagtgaa agcgattgtc ctgcgtgtta
     5041 atagcccagg cggcagcgtt accgcgtctg aagtgattcg cgctgaactg gcagcagccc
     5101 gggcagcggg taagcctgtg gttgtatcga tgggcggcat ggcggcatct ggtggttact
     5161 ggatttccac gccagctaat tacattgtgg ctaaccccag caccctgacc ggttctatcg
     5221 gtatcttcgg cgtgatcacc accgtagaaa atagtctgga ttcgattggt gttcatactg
     5281 atggtgtctc aacttcaccg ctggcggatg tttctatcac cagggcactg ccgccggaag
     5341 cgcagcagat gatgcaatta agcattgaga atggctataa acgctttatc acgctggttg
     5401 ctgatgcgcg tcattcgacg ccggagcaaa ttgataaaat cgcccagggc cacgtctgga
     5461 ccggtcagga tgcaaaagct aacgggctgg tcgatagtct cggggatttc gatgatgcgg
     5521 ttgccaaagc agcagagctg gcaaaagtga aacagtggca tctggaatac tacgttgatg
     5581 aaccgacctt cttcgacaaa gtgatggaca acatgtctgg ttctgtccgg gcaatgttgc
     5641 cagatgcgtt ccaggccatg ttacctgcac cgcttgcctc ggtagcctct accgttaaaa
     5701 gtgaaagcga caagctggcc gcgtttaacg acccacaaaa ccgttatgcg ttttgcctga
     5761 cctgcgccaa cgtgcgttaa gtcttgtact gagtggccga cagatcgtcg gccacattat
     5821 tttttacctc gacgaatcct cttcccgctg tctcgaccca tatttcctta tccgcatagt
     5881 atcaggtgcg ctcctcctgc ctcacgtata tacttttgct ctttcgatat cattcatatc
     5941 aatatcatgc aaaagaaatc aatttacgtc gcctacacgg gcgggaccat cgggatgcag
     6001 cgttccgagc agggttatat tccggtgtca ggtcatctac aacgccaact ggcgctgatg
     6061 ccggaattcc atcgcccgga gatgccagat ttcaccattc atgaatatac gccgttgatg
     6121 gattcttctg atatgacgcc ggaagactgg cagcatattg ctgaagatat taaagcgcac
     6181 tatgacgact atgatggttt tgtcattcta cacggtaccg acacgatggc gtataccgcc
     6241 tctgcgctgt cgttcatgct cgagaatctc ggtaaaccgg tcattgtgac agggtcacaa
     6301 atcccgctgg ctgagttacg ctctgacgga caaattaatc tgctgaatgc gttgtacgtt
     6361 gcggcgaatt atccgatcaa cgaagtaacg ctctttttca ataaccgatt gtatcgcggc
     6421 aaccgcacta ccaaagccca tgccgatggt tttgatgcgt ttgcctctcc aaaccttcct
     6481 ccgttactgg aagctggtat ccatattcgt cgtttgaata cgccacccgc cccgcacggt
     6541 gaaggggaat taatcgttca tccaatcacg ccacaaccaa ttggcgtagt gacgatttat
     6601 ccagggattt ctgctgacgt cgtgcgcaat tttctgcgcc aaccggtgaa agcattgatt
     6661 ctgcgctcct atggcgtggg taatgcgcca caaaacaaag ccttcctgca ggaattacaa
     6721 gaagccagcg atcgcggtat tgtggtggtc aacctgacac aatgtatgtc cggtaaagtg
     6781 aatatgggtg gttatgccac cggtaacgcc ctcgcccatg ccggcgttat tggcggtgca
     6841 gatatgactg tagaagccac gctaaccaaa ctgcattacc tgctgagcca ggaactggat
     6901 actgaaacca ttcgcaaggc catgagccaa aacctgcgcg gcgaactgac gccggatgat
     6961 taaggagact gcaatgcccc atcgcgccct gttactggtc gatttacaaa atgatttctg
     7021 tgctggtggc gcgctcgccg tgccggaagg tgacagtacg gtagatgtcg ctaaccgcct
     7081 gattgactgg tgccagtcgc gcggtgaagc ggttatcgcc agtcaggact ggcacccggc
     7141 gaatcacggc agttttgcca gtcagcacgg tgtagagcct tatacgccag gccaactcga
     7201 cggtttgcca caaaccttct ggccagatca ctgtgtgcag aacagtgaag gcgcacaatt
     7261 acatccgtta ctgaaccaaa aagcgatcgc agcggtgttc cataaaggcg aaaatccttt
     7321 agttgacagt tacagtgcct tttttgataa cggccgtcgg cagaaaacct ctctcgatga
     7381 ctggttacgc gatcatgaaa tcgatgaatt gatcgttatg ggcctggcta ctgactattg
     7441 cgtgaagttt accgtgctgg acgcgttaca gttaggttat aaggtaaacg tgattaccga
     7501 tggttgtcgt ggcgtgaata tccagcccca ggacagtgcg cacgcgttta tggagatgtc
     7561 agcagctggg gcaacgctat atacgctggc agactgggaa gagacgcagg ggtaatatta
     7621 gttgaattca tctctggcct acatttcctc aatgacattg taggccagat aatacttgtc
     7681 agtgttgcat ttaacctact tgcagctaaa acacgacgtc ctaacaagta tatttgccct
     7741 cccttaataa caggcttaaa tactagataa ctcattcact taaacatagc aataggcttt
     7801 gctgagatac caaaatcttc ttttaactgc tgtttgctct tcatgaccat ctgaccattt
     7861 gtgtcaattg tcatatgctg tgcttcggta ttatggcggg cttgccacag catcaccagt
     7921 tgcaggcaat tctctttttg ttcctcggtc aacgcaacgc catcaggcca tttccccagt
     7981 tcaacggcgg tcgacaaacg ctggtatacc tcaggcgtca tgctgttgat aatgtcatca
     8041 agattcataa tttttccgct cctgtggaat aagttgctga atcgtttttt caaccgttga
     8101 tttcttcgcc gttttcgcca tcagtgaagc gtaaagaagc agagttaaca caataacgtt
     8161 cgcctgttgg ttgcggcccg tcgggaaaga catgccccag atgggcatca cagttaccgc
     8221 aacgaatttc tatgcgctgc attccatgtg acaagtcttt gatataacga atggattctt
     8281 cactcaccgg ttcgtagaaa ctgggccagc cacagccgga atcatacttg gtttcggaat
     8341 gaaacagcgg ggcatcgcag atcaaacagt gatatacgcc gtcacgcttg ttatgcagta
     8401 aacgacccgt aaatggcggt tctgtcccat gattctgcgt cacgtaaaac tgcatctcgg
     8461 acaaattttt tttcagttct tctgccgaag gtttattagc catttgctca catctcactt
     8521 taatcgtgct cacattacgt gactgattct aacaaaacat taacaccaac tggcaaaatt
     8581 ttgtcctaaa cttgatctcg acgaaatggc tgcacctaaa tcgtgatgaa aatcacattt
     8641 ttatcgtaat tgccctttaa aattcggggc gccgacccca tgtggtctca agcccaaagg
     8701 aagagtgagg cgagtcagtc gcgtaatgct taggcacagg attgatttgt cgcaatgatt
     8761 gacacgattc cgcttgacgc tgcgtaaggt ttttgtaatt ttacaggcaa ccttttattc
     8821 actaacaaat agctggtgga atatatgact atcaaagtag gtatcaacgg ttttggccgt
     8881 atcggtcgca ttgttttccg tgctgctcag aaacgttctg acatcgagat cgttgcaatc
     8941 aacgacctgt tagacgctga ttacatggca tacatgctga aatatgactc cactcacggc
     9001 cgtttcgacg gtaccgttga agtgaaagac ggtcatctga tcgttaacgg taaaaaaatc
     9061 cgtgttaccg ctgaacgtga tccggctaac ctgaaatggg acgaagttgg tgttgacgtt
     9121 gtcgctgaag caactggtct gttcctgact gacgaaactg ctcgtaaaca catcaccgct
     9181 ggtgcgaaga aagtggttat gactggtccg tctaaagaca acactccgat gttcgttaaa
     9241 ggcgctaact tcgacaaata tgctggccag gacatcgttt ccaacgcttc ctgcaccacc
     9301 aactgcctgg ctccgctggc taaagttatc aacgataact tcggcatcat cgaaggtctg
     9361 atgaccaccg ttcacgctac taccgctact cagaaaaccg ttgatggccc gtctcacaaa
     9421 gactggcgcg gcggccgcgg cgcttcccag aacatcatcc cgtcctctac cggtgctgct
     9481 aaagctgtag gtaaagtact gccagaactg aatggcaaac tgactggtat ggcgttccgc
     9541 gttccgaccc cgaacgtatc cgtagttgac ctgaccgttc gtctggaaaa agctgcaact
     9601 tacgagcaga tcaaagctgc cgttaaagct gctgctgaag gcgaaatgaa aggcgttctg
     9661 ggctacaccg aagatgacgt agtatctacc gatttcaacg gcgaagtttg cacttccgtg
     9721 ttcgatgcta aagctggtat cgctctgaac gacaacttcg tgaaactggt atcttggtac
     9781 gacaacgaaa ccggttactc caacaaagtt ctggacctga tcgctcacat ctccaaataa
     9841 gttgagatga cactgtgatc taaaaagagc gacttcggtc gctctttttt ttacctgata
     9901 aaatgaagtt aaaggactgc gtcatgatta agaaaatttt tgcccttccg gtcatcgaac
     9961 aaatctcccc tgtcctctcc cgtcgtaaac tggatgaact ggacctcatt gtggtcgatc
    10021 atccccaggt aaaagcctct tttgcattac agggcgcaca ccttctctcg tggaaacctg
    10081 cgggtgaaga agaagttctg tggttgagca acaacacacc gttcaaaaat ggcgtcgcta
    10141 ttcgcggtgg cgtaccggtt tgctggccgt ggtttggtcc ggcggcacaa caaggtctgc
    10201 ctgcgcacgg ttttgcccgc aacctgccgt ggacgctgaa atcacatcgt gaagatgctg
    10261 atggcgtagc gctgactttt gaattgacgc aaagcgaaga gacgaaaaaa ttctggccgc
    10321 acgactttac gctgttagcg catttccgcg tgggtaaaac ctgtgaaatc gatcttgaat
    10381 cacatggcga atttgaaacc acctctgccc tgcataccta ctttaacgtg ggtgatatcg
    10441 ctaaggtaag cgtcagtggg ctgggcgatc gcttcattga taaagtaaat gacgcgaaag
    10501 aagatgtact gaccgacggt attcagacct tccctgaccg taccgatcgc gtgtatctga
    10561 atccgcaaga ttgcagcgtg attaatgatg aagcgctgaa tcgtattatc gccgtaggcc
    10621 accagcatca tctgaacgtt gtcggctgga acccgggacc ggcgctttca gttagcatgg
    10681 gcgacatgcc ggatgatggc tacaaaacat ttgtttgtgt agaaacggct tacgcttcag
    10741 aaacgcaaaa agtgaccaaa gagaaacctg cacatctggc gcaatccatt cgcgttgcga
    10801 aacgttaatt tgcgttaatg ttgtgtgccg ggtgcgatgc atccggcacg tatcaacatc
    10861 acaccatatc cagcgcagtt tttccttttg gtgccggata cgctttatcc agcatggtta
    10921 attccgctga cgaaagttcg acctcaagca cagccgcatt ctgttggaca tgggcaatcg
    10981 tggccgcttt tggaatcgcc atcacaccct gatggctgat tacccacgcc aacaatactt
    11041 gtgccgcgct gatattgtga gcatgtgcaa tttcgttgac taccgcgttt tttaacagtc
    11101 cattgcgcaa ccgcccggcc tgggctaatg gactgtaagc catcaccggc atctgctgtt
    11161 gctggcacca ggggagtaga tcgtactcaa tccctcgtga accgagatgg taaagcacct
    11221 gattagtggc acactgattt ccccccggca gctgccagag ttcctgcata tcagcatgat
    11281 caaggttaga aacgccccag cggcggattt ttccctgggc gatcaatttt tccatcgctg
    11341 ccacagtctc ttcaaaagcg aaactgccag accagtgtaa taagtagaga tcgagataat
    11401 cagtattgag acggcgtaaa ctggcttcgc atgcatttat cgctttttgc ccgccagcat
    11461 tccacggata gactttagag acgagaaaga cgttatcgcg cagaccggtt aatgcttccc
    11521 caaccacctt ttcggcaccg ccatcggcat acatttcggc ggtatcaatg agggttaaac
    11581 cgagctcaat gcccgcgcgt agtgcagcaa cttctgtttt gcgctgactg gcatcttcgc
    11641 ccatatacca tgttccctgc cctatggctg gcagtgagac atcgccacta aattgaatca
    11701 ttttttgttg cattgtttcc tcccaggtat tgcaccaccg taatgcaaaa cagggcgtga
    11761 cgccctgttt ttatgcacaa aatgccctgg aaagatgcat tatcagaatt tgtaggtgat
    11821 cccggtagaa atcaggccag tccaggattt atccaccatc gggctgtcag taacttcatc
    11881 agacagacgg gtatagcgcg cggtaccgta aacactccag tcgccgagga agttgtagct
    11941 ggcgctcagc tccaggtaag ggctccagct gtcgttcggg ttatagccac gcagaccgct
    12001 gcgagcggac tctttgcgcg atacgccata atagtattcg ttctggtttt cgctgttcca
    12061 ctgcacaccg atacccgggg tcacggtcag gccgccgttg gtgtaacgat acaaccaggc
    12121 catatcccag acgatgccgt tgctgttatc cagggtatcg ccagccaggg tggtacgcag
    12181 gtaaccgtac tgggtaaagt gagcataaga cagaccagcc atcatggtgc tcttacggtc
    12241 atccaggtga cgcatttggt gatcgccact gtctttcgct ttgaagtaaa gcggcgacca
    12301 ataagcggta attgaaagtt tgtccgttgc gtcattccac aggtagtaac caccacctaa
    12361 gccacggaac cagaagttat cgccttcata gttgattacc ggtactgggt aaacatcggt
    12421 atcgtaatct ttatatgggt gctcaacgac acctacgcct gcgcccaggg aaaatttacc
    12481 ttcagcgtgc gctacgcctg cagacgttgc gataagcact ccaagtgcca gaagtttgag
    12541 tttggtcaca attaatcatt ccttaaacaa atgtttagcg ggcgacaaag tttacccgtc
    12601 aatacatcga aacccaacct ttttacgttt tcatttttta aagtaactgt ttaattttcc
    12661 tgacgcggat gacaccgcgc ttacagccaa atgaatttag cgttactggc taccctggtc
    12721 tttacattaa ttatgcaaaa tttatggatg agttgttgat atgccattga aattaagaaa
    12781 gccgtgcagg caagttttcc atttgccatc tacgcttaat tttgaaggtg tatcaccggg
    12841 cacgttgttc tcatcgtcga taaaatggca tgagagttgc tgtgttttag caagagacgt
    12901 cgttcagttt acctcttccg ggagcctcta ctattcatat gaacggctct taacctgtgc
    12961 taaaaaacga aaggacggca taccatgaat atattcgatc actatcgcca gcgatatgaa
    13021 gctgccaagg acgaagagtt cacactgcag gagtttctta ccacttgtcg gcaagatcgc
    13081 agtgcttatg ccaacgcggc tgagcggcta ttgatggcta tcggtgagcc tgtcatggtc
    13141 gatacagccc aggaacccag actttctcga ctcttttcta accgggtcat tgcacgttat
    13201 ccggcatttg aagagtttta cggcatggaa gacgcgattg aacagattgt ctcttatctg
    13261 aaacacgcgg ctcaggggct ggaagagaag aaacaaatcc tgtatctgct ggggcctgtg
    13321 ggtgggggta aatcatcgct tgctgagcga ctgaaatcat taatgcagct cgtaccgatt
    13381 tatgtattga gcgccaacgg tgagcgtagc ccggtcaacg atcatccgtt ctgtcttttc
    13441 aatccgcagg aagatgctca gattctggaa aaagagtatg gcattcctcg ccgttatctc
    13501 ggcaccatca tgtcgccatg ggcggcaaaa cgcctgcatg aatttggtgg cgatatcact
    13561 aagttccggg tagtgaaggt ctggccgtca attctgcaac aaattgctat cgccaaaacg
    13621 gaacccggtg atgagaacaa ccaggacatc tccgcgctgg ttgggaaagt cgatattcgt
    13681 aaactcgaac actacgcgca gaatgacccg gacgcctacg gctattccgg tgcgctgtgc
    13741 cgcgccaacc aagggatcat ggaatttgtc gagatgttta aagcaccgat aaaagtgctg
    13801 catccgctgt taaccgccac tcaggaaggt aactacaacg ggacggaagg tatctccgcc
    13861 ctgccgttca acgggattat tctcgcccac tcgaacgaat ccgaatgggt cactttccgt
    13921 aataacaaaa acaacgaagc cttcctcgac cgtgtttaca tcgtgaaggt gccgtattgc
    13981 ttacgcattt ccgaagagat caaaatctac gagaaattgc ttaatcacag tgaattgact
    14041 cacgccccct gcgcccctgg cacgctggaa acgctgtcac gtttttccat tctttcgcgc
    14101 cttaaagagc cagaaaactc cagcatttat tcaaagatgc gggtttatga tggcgaaagt
    14161 ctgaaagaca ccgatcccaa agccaagtcg tatcaggaat atcgtgacta cgccggtgtc
    14221 gatgaaggga tgaacggtct gtcgacgcgt tttgcgttta agatcctctc ccgcgtgttc
    14281 aacttcgatc atgtagaagt ggcggcaaac ccggtccatc tgttctacgt cctggaacag
    14341 cagatcgagc gcgagcagtt cccacaagag caggcagaac gctatctgga gttcctgaaa
    14401 ggttatctga tcccgaaata tgccgagttt atcggcaaag agatccagac ggcctacctt
    14461 gaatcctatt ccgaatatgg gcaaaacatt ttcgaccgtt atgttaccta cgcggatttc
    14521 tggattcagg atcaggagta tcgcgatccg gataccgggc agctgtttga ccgcgagtct
    14581 cttaacgccg agctggagaa aatcgagaaa ccggcgggga tcagtaatcc aaaagatttc
    14641 cgcaacgaga ttgttaactt cgtactgcgc gccagagcga ataacagcgg acgcaatccg
    14701 aactggacca gctatgaaaa actgcgcacg gttatcgaga agaaaatgtt ctccaatacc
    14761 gaggagctgt tgccggttat ctcgtttaac gccaaaacgt caaccgacga gcagaagaaa
    14821 cacgacgact ttgtcgaccg tatgatggaa aaaggctaca cccgtaaaca ggtgcgttta
    14881 ctgtgcgaat ggtatttgcg cgtacgtaaa tcgtcttaac aaccctggcc cggtacacat
    14941 gttaccgggc ctacaacgac agcgaaccgt gggcctgaga agcggcaaca caggcgtagc
    15001 atacagttgg caaatgtagt acggggggca tatgacctgg tttattgacc ggcgtctgaa
    15061 cggcaaaaac aaaagcatgg tgaatcgcca gcgtttttta cgccgttata aagcgcaaat
    15121 taaacagtcg atctccgagg ccattaataa gcgttcggtg actgacgtcg acagcggcga
    15181 atccgtatcc attcccacgg aagatattag cgaaccgatg tttcatcagg ggcgtggcgg
    15241 tctgcgccac cgcgtgcatc cgggcaatga ccatttcgtc cagaacgacc gaattgaacg
    15301 tccccagggt ggcggcggag gttccggtag tggtcagggc caggccagcc aggatggtga
    15361 aggtcaggat gaatttgtct ttcagatttc gaaagatgag tatcttgatt tgctctttga
    15421 agatttggcc ttaccgaatc tgaaacaaaa ccaacaacgc cagctgaccg aatataaaac
    15481 gcatcgggcg ggttataccg ctaacggcgt tccggcaaat atcagcgttg tgcgctcctt
    15541 gcagaactca ctggcgcgac gcacagccat gacggcaggc aagcggcggg aacttcatgc
    15601 actggaagag aatttggcca tcatcagcaa cagtgaacct gcgcagctgc tggaagagga
    15661 acgtctgcgc aaagaaattg cagaattacg tgccaaaatt gaacgcgtcc cttttattga
    15721 caccttcgat ttacgttaca agaactacga gaagcggccc gatccctcca gccaggcagt
    15781 gatgttttgc ctgatggacg tttccggttc aatggatcaa tccactaaag atatggctaa
    15841 gcgtttttat attctgctgt atctgttcct cagcagaacg tataagaacg tggaagtcgt
    15901 atacatccgc catcataccc aggcgaaaga agtcgatgaa catgagtttt tctactcgca
    15961 ggaaacaggc ggcaccattg tttccagcgc cctgaaactg atggatgagg tagtgaaaga
    16021 gcgttataac ccggcacagt ggaatattta cgctgcacaa gcatcggacg gcgataactg
    16081 ggccgatgac tctccgcttt gccatgaaat cctggcgaaa aaattattac ctgttgttcg
    16141 ttattacagc tatatcgaaa ttacccgtcg tgcacatcag acattgtggc gagaatatga
    16201 gcatctgcaa tctactttcg acaactttgc gatgcagcac atccgcgacc aggatgatat
    16261 ttatccggtg ttccgtgaac tgtttcataa acaaaatgca acagctaaag actaaaacta
    16321 tcagccaggt cattatcgcc tggctgattt t
//
LOCUS       DAZKHF020000084        16273 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2201S31241
            SAMN43267800-rid25106413.denovo.085, whole genome shotgun sequence.
ACCESSION   DAZKHF020000084 DAZKHF020000000
VERSION     DAZKHF020000084.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267800
            Sequence Read Archive: SRR30307263
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 16273)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16273)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 16273)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350581.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350581.2
            Genome Coverage       :: 151x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:21:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,652
            CDSs (total)                      :: 5,557
            Genes (coding)                    :: 5,320
            CDSs (with protein)               :: 5,320
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 237
            CDSs (without protein)            :: 237
            Pseudo Genes (ambiguous residues) :: 0 of 237
            Pseudo Genes (frameshifted)       :: 102 of 237
            Pseudo Genes (incomplete)         :: 130 of 237
            Pseudo Genes (internal stop)      :: 52 of 237
            Pseudo Genes (multiple problems)  :: 41 of 237
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16273
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267800-rid25106413.denovo.085"
                     /serotype="O 26:H11"
                     /isolate="2201S31241"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-01"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            complement(33..1487)
                     /gene="xylB"
                     /locus_tag="ACHOSC_RS11395"
                     /old_locus_tag="pgaptmp_002279"
     CDS             complement(33..1487)
                     /gene="xylB"
                     /locus_tag="ACHOSC_RS11395"
                     /old_locus_tag="pgaptmp_002279"
                     /EC_number="2.7.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004856 - D-xylulokinase activity
                     [Evidence IEA];
                     GO_process: GO:0005997 - xylulose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="xylulokinase"
                     /protein_id="HGU4548209.1"
                     /translation="MYIGIDLGTSGVKVILLNEQGEVVASQTEKLTVSRPHPLWSEQD
                     PEQWWQATDRAMKALGDQHSLQDVKALGIAGQMHGATLLDAQQRVLRPAILWNDGRCA
                     QECTLLEARVPQSRVITGNLMMPGFTAPKLLWVQRHEPEIFRQIDKVLLPKDYLRLRM
                     TGEFASDMSDAAGTMWLDVAKRDWSDVMLQACDLSRDQMPALYEGSEITGALLPEVAK
                     AWGMATVPVVAGGGDNAAGAVGVGMVDANQAMLSLGTSGVYFAVSEGFLSKPESAVHS
                     FCHALPQRWHLMSVMLSAASCLDWVAKLTGLCNVPALIAAAQQADESAEPVWFLPYLS
                     GERTPHNNPQAKGVFFGLTHQHGPNELARAVLEGVGYALADGMDVVHACGIKPQSVTL
                     IGGGARSEYWRQMLADISGQQLDYRTGGDVGPALGAARLAQIAANPEKSLIELLPQLP
                     LEQSHLPDAQRYAAYQPRRETFRRLYQQLLPLMA"
     gene            complement(1559..2881)
                     /gene="xylA"
                     /locus_tag="ACHOSC_RS11400"
                     /old_locus_tag="pgaptmp_002280"
     CDS             complement(1559..2881)
                     /gene="xylA"
                     /locus_tag="ACHOSC_RS11400"
                     /old_locus_tag="pgaptmp_002280"
                     /EC_number="5.3.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009045 - xylose isomerase activity
                     [Evidence IEA];
                     GO_process: GO:0042732 - D-xylose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="xylose isomerase"
                     /protein_id="HGU4548210.1"
                     /translation="MQAYFDQLDRVRYEGSKSSNPLAFRHYNPDELVLGKRMEEHLRF
                     AACYWHTFCWNGADMFGVGAFNRPWQQPGEALALAKRKADVAFEFFHKLHVPFYCFHD
                     VDVSPEGASLKEYINNFAQMVDVLAAKQEESGVKLLWGTANCFTNPRYGAGAATNPDP
                     EVFSWAATQVVTAMEATHKLGGENYVLWGGREGYETLLNTDLRQEREQLGRFMQMVVE
                     HKHKIGFQGTLLIEPKPQEPTKHQYDYDAATVYGFLKQFGLEKEIKLNIEANHATLAG
                     HSFHHEIATAIALGLFGSVDANRGDAQLGWDTDQFPNSVEENALVMYEILKAGGFTTG
                     GLNFDAKVRRQSTDKYDLFYGHIGAMDTMAVALKIAARMIEDGELDKRIAQRYSGWNS
                     ELGQQILKGQMSLADLAKYAQEHNLSPVHQSGRQEQLENLVNHYLFDK"
     gene            3247..4239
                     /gene="xylF"
                     /locus_tag="ACHOSC_RS11405"
                     /old_locus_tag="pgaptmp_002281"
     CDS             3247..4239
                     /gene="xylF"
                     /locus_tag="ACHOSC_RS11405"
                     /old_locus_tag="pgaptmp_002281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015753 - D-xylose transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-xylose ABC transporter substrate-binding
                     protein"
                     /protein_id="HGU4548211.1"
                     /translation="MKIKNILLTLCTSLLLTNVAAHAKEVKIGMAIDDLRLERWQKDR
                     DIFVKKAESLGAKVFVQSANGNEETQMSQIENMINRGVDVLVIIPYNGQVLSNVVKEA
                     KQEGIKVLAYDRMINDADIDFYISFDNEKVGELQAKALVDIVPQGNYFLMGGSPVDNN
                     AKLFRAGQMKVLKPYVDSGKIKVVGDQWVDGWLPENALKIMENALTANNNKIDAVVAS
                     NDATAGGAIQALSAQGLSGKVAISGQDADLAGIKRIAAGTQTMTVYKPITLLANTAAE
                     IAVELGNGQEPKADTTLNNGLKDVPSRLLTPIDVNKNNIKDTVVKDGFHKESEL"
     gene            4317..5858
                     /gene="xylG"
                     /locus_tag="ACHOSC_RS11410"
                     /old_locus_tag="pgaptmp_002282"
     CDS             4317..5858
                     /gene="xylG"
                     /locus_tag="ACHOSC_RS11410"
                     /old_locus_tag="pgaptmp_002282"
                     /EC_number="7.5.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015614 - ABC-type D-xylose transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015753 - D-xylose transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-xylose ABC transporter ATP-binding protein"
                     /protein_id="HGU4548212.1"
                     /translation="MPYLLEMKNITKTFGSVKAIDNVCLRLNADEIVSLCGENGSGKS
                     TLMKVLCGIYPHGSYEGEIIFAGEEIQASHIRDTERKGIAIIHQELALVKELTVLENI
                     FLGNEITHNGIMDYDLMTLRCQKLLAQVSLSISPDTRVGDLGLGQQQLVEIAKALNKQ
                     VRLLILDEPTASLTEQETSVLLDIIRDLQQHGIACIYISHKLNEVKAISDTICVIRDG
                     QHIGTRDAAGMSEDDIITMMVGRELTALYPNEPHTTGDEILRIEHLTAWHPVNRHIKR
                     VNDVSFSLKRGEILGIAGLVGAGRTETIQCLFGVWPGQWEGKIYIDGKQVDIRNCQQA
                     IAQGIAMVPEDRKRDGIVPVMAVGKNITLAALNKFTGGISQLDDAEEQKCILESIQQL
                     KVKTSSPDLAIGRLSGGNQQKAILARCLLLNPRILILDEPTRGIDIGAKYEIYKLINQ
                     LVQQGIAVIVISSELPEVLGLSDRVLVMHEGKLKANLINHNLTQEQVMEAALRSEHHV
                     EKQSV"
     gene            5836..7017
                     /gene="xylH"
                     /locus_tag="ACHOSC_RS11415"
                     /old_locus_tag="pgaptmp_002283"
     CDS             5836..7017
                     /gene="xylH"
                     /locus_tag="ACHOSC_RS11415"
                     /old_locus_tag="pgaptmp_002283"
                     /EC_number="7.5.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xylose ABC transporter permease XylH"
                     /protein_id="HGU4548213.1"
                     /translation="MSKSNPSEVKLAVPTSGGFSGLKSLNLQVFVMIAAIIAIMLFFT
                     WTTDGAYLSARNVSNLLRQTAITGILAVGMVFVIISAEIDLSVGSMMGLLGGVAAICD
                     VWLGWPLPLTIIVTLVLGLLLGAWNGWWVAYRKVPSFIVTLAGMLAFRGILIGITNGT
                     TVSPTSAAMSQIGQSYLPASTGFIIGALGLMAFVGWQWRGRMRRQALGLQSPASTAVV
                     GRQALTAIIVLGAIWLLNDYRGVPTPVLLLTLLLLGGMFMATRTAFGRRIYAIGGNLE
                     AARLSGINVERTKLAVFAINGLMVAIAGLILSSRLGAGSPSAGNIAELDAIAACVIGG
                     TSLAGGVGSVAGAVMGAFIMASLDNGMSMMDVPTFWQYIVKGAILLLAVWMDSATKRR
                     S"
     gene            7095..8273
                     /gene="xylR"
                     /locus_tag="ACHOSC_RS11420"
                     /old_locus_tag="pgaptmp_002284"
     CDS             7095..8273
                     /gene="xylR"
                     /locus_tag="ACHOSC_RS11420"
                     /old_locus_tag="pgaptmp_002284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312479.1"
                     /note="D-xylose enhances binding of XylR to the xyl
                     promoter and activates transcription; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-xylose utilization transcriptional activator
                     XylR"
                     /protein_id="HGU4548214.1"
                     /translation="MFTKRHRITLLFNANKAYDRQVVEGVGEYLQASQSEWDIFIEED
                     FRARIDKIKDWLGDGVIADFDDKQIEQALADVDVPIVGVGGSYHLAESYPPVHYIATD
                     NYALVESAFLHLKEKGVNRFAFYGLPESSGKRWATEREYAFRQLVAEEKYRGVVYQGL
                     ETAPENWQHAQNRLADWLQTLPPQTGIIAVTDARARHILQVCEHLHIPVPEKLCVIGI
                     DNEELTRYLSRVALSSVAQGARQMGYQAAKLLHRLLDKEEMPLQRILVPPVRVIERRS
                     TDYRSLTDPAVIQAMHYIRNHACKGIKVDQVLDAVGISRSNLEKRFKEEVGETIHAMI
                     HAEKLEKARSLLISTTLSINEISQMCGYPSLQYFYSVFKKAYDTTPKEYRDVNSEVML
                     "
     gene            complement(8381..9205)
                     /locus_tag="ACHOSC_RS11425"
                     /old_locus_tag="pgaptmp_002285"
     CDS             complement(8381..9205)
                     /locus_tag="ACHOSC_RS11425"
                     /old_locus_tag="pgaptmp_002285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein bax"
                     /protein_id="HGU4548215.1"
                     /translation="MILTPIRRYGAMILMLLTLVFSSEVLAKTHTTTASQKSHLTKAS
                     NKQVSSKQEYSRNSAKSNSLPDLRKYPSGTPRKKAFLRTVMPYITSQNAAITAERNWL
                     ISKQYQGQWSPAERARLKDIAKRYKVKWSGNTRKIPWNTLLERVDIIPTSMVATMAAA
                     ESGWGTSKLARNNNNLFGMKCMKGRCTNAPGKVKGYSQFSSVKESVSAYVTNLNTHPA
                     YSSFRKSRAQLRKADQEVTATAMIHKLKGYSTKGKSYNNYLFAMYQDNQRLIAAHM"
     gene            9525..11555
                     /gene="malS"
                     /locus_tag="ACHOSC_RS11430"
                     /old_locus_tag="pgaptmp_002286"
     CDS             9525..11555
                     /gene="malS"
                     /locus_tag="ACHOSC_RS11430"
                     /old_locus_tag="pgaptmp_002286"
                     /EC_number="3.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004556 - alpha-amylase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase"
                     /protein_id="HGU4548216.1"
                     /translation="MKLAACFLTLLPGFAVAASWTSPGFPAFSEQGTETFVSHAQLPK
                     GTRPLTLNFDQQCWQPADAIKLNQMLSLQPCSNTQPQWRLFRDGEYTLQIDTRSGTPT
                     LMISIQNAAEPVASQVRVCPKWDGLPLTLDVSATFPEGAAVRDYYSQQIAIVKNGQIT
                     LQPAATSNGLLLLERAETDASAPFDWHNATVYFVLTDRFENGDPSNDQSYGRHKDGMA
                     EIGTFHGGDLRGLTNKLDYLQQLGVNALWISAPFEQIHGWVGGGTKGDFPHYAYHGYY
                     TQDWTNLDANMGNEADLRTLVDSAHQRGIRILFDVVMNHTGYATLADMQEYQFGALYL
                     SGDEVKKTLGERWSDWKPAAGQTWHSFNDYINFSDKTGWDKWWGKNWIRTDIGDYDNP
                     GFDDLTMSLAFLPDIKTESTTASGLPVFYKNKTDTHAKAIDGFTPRDYLTHWLSQWVR
                     DYGIDGFRVDTAKHVELPAWQQLKTEASAALREWKKANPDKALDDKPFWMTGEAWGHG
                     VMQSDYYRHGFDAMINFDYQEQAAKAVDCIAQMDMTWQQMAEKLQGFNVLSYLSSHDT
                     RLFREGGDKAAELLLLAPGAVQIFYGDESSRPFGPTGSDPLQGTRSDMNWQDVSGKSA
                     ANVAHWQKISQFRARHPAIGAGKQTTLSLKQGYGFVREHGDDKVLVIWAGQQ"
     gene            11733..12986
                     /gene="avtA"
                     /locus_tag="ACHOSC_RS11435"
                     /old_locus_tag="pgaptmp_002287"
     CDS             11733..12986
                     /gene="avtA"
                     /locus_tag="ACHOSC_RS11435"
                     /old_locus_tag="pgaptmp_002287"
                     /EC_number="2.6.1.66"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="valine--pyruvate transaminase"
                     /protein_id="HGU4548217.1"
                     /translation="MTFSLFGDKFTRHSGITLLMEDLNDGLRTPGAIMLGGGNPAQIP
                     EMQDYFQTLLTDMLESGKATDALCNYDGPQGKTELLTLLAGMLREKLGWDIEPQNIAL
                     TNGSQSAFFYLFNLFAGRRADGRVKKVLFPLAPEYIGYADAGLEEDLFVSARPNIELL
                     PEGQFKYHVDFEHLHIGEETGMICVSRPTNPTGNVITDEELLKLDALANQHGIPLVID
                     NAYGVPFPGIIFSEARPLWNPNIVLCMSLSKLGLPGSRCGIIIANEKIITAITNMNGI
                     ISLAPGGIGPAMMCEMIKRNDLLRLSETVIKPFYYQRVQETIAIIRRYLPENRCLIHK
                     PEGAIFLWLWFKDLPITTEQLYQRLKARGVLMVPGHNFFPGLDKPWPHTHQCMRMNYV
                     PEPEKIEAGVKILAEEIERAWAESH"
     gene            complement(13138..13611)
                     /locus_tag="ACHOSC_RS11440"
                     /old_locus_tag="pgaptmp_002288"
     CDS             complement(13138..13611)
                     /locus_tag="ACHOSC_RS11440"
                     /old_locus_tag="pgaptmp_002288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HGU4548218.1"
                     /translation="MNRFIIADATKCIGCRTCEVACAVSHQENQDCAALSPDEFISRI
                     RVIKDHSWTTAVACHQCEDAPCANVCPVDAISREHGHIFVEQTRCIGCKSCMLACPFG
                     AMEVVSSRKKARAIKCDLCWHRETGPACVEACPTKALQCMDVEKVQRHRLRQQPV"
     gene            complement(13713..14561)
                     /gene="yiaJ"
                     /locus_tag="ACHOSC_RS11445"
                     /old_locus_tag="pgaptmp_002289"
     CDS             complement(13713..14561)
                     /gene="yiaJ"
                     /locus_tag="ACHOSC_RS11445"
                     /old_locus_tag="pgaptmp_002289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IclR family transcriptional regulator YiaJ"
                     /protein_id="HGU4548219.1"
                     /translation="MGKEVMGKKENEMAQEKERPAGSQSLFRGLMLIEILSNYPNGCP
                     LAHLSELAGLNKSTVHRLLQGLQSCGYVTTAPAAGSYRLTTKFIAVGQKALSSLNIIH
                     IAAPHLEALNIATGETINFSSREDDHAILIYKLEPTTGMLRTRAYIGQHMPLYCSAMG
                     KIYMAFGHPDYVKSYWENHQHEIQPLTRNTITELPAMFDELAHIRESGAAMDREENEL
                     GVSCIAVPVFDIHGRVPYAVSISLSTSRLKQVGEKNLLKPLRETAQAISNELGFTVRD
                     DLGAIT"
     gene            14762..15760
                     /gene="yiaK"
                     /locus_tag="ACHOSC_RS11450"
                     /old_locus_tag="pgaptmp_002290"
     CDS             14762..15760
                     /gene="yiaK"
                     /locus_tag="ACHOSC_RS11450"
                     /old_locus_tag="pgaptmp_002290"
                     /EC_number="1.1.1.130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047559 - 3-dehydro-L-gulonate
                     2-dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-dehydro-L-gulonate 2-dehydrogenase"
                     /protein_id="HGU4548220.1"
                     /translation="MKVTFEQLKAAFNRVLISRGVDSETADACAEMFARTTESGVYSH
                     GVNRFPRFIQQLENGDIIPDAQPKRITSLGAIEQWDAQRSIGNLTAKKMMDRAIELAA
                     DHGIGLVALRNANHWMRGGSYGWQAAEKGYIGICWTNSIAVMPPWGAKECRIGTNPLI
                     VAIPSTPITMVDMSMSMFSYGMLEVNRLAGRQLPVDGGFDDEGNLTKEPGVIEKNRRI
                     LPMGYWKGSGMSIVLDMIATLLSDGASVAEVTQDNSDEYGISQIFIAIEVDKLIDGPT
                     RDAKLQRIMDYVTTAERADENQAIRLPGHEFTTLLAENRRNGITVDDSVWAKIQAL"
     gene            15772..16239
                     /locus_tag="ACHOSC_RS11455"
                     /old_locus_tag="pgaptmp_002291"
     CDS             15772..16239
                     /locus_tag="ACHOSC_RS11455"
                     /old_locus_tag="pgaptmp_002291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhcH/YjgK/YiaL family protein"
                     /protein_id="HGU4548221.1"
                     /translation="MIFGHIAQPNPCRLPAAIEKALDFLRATDFNALEPGVVEIDGKN
                     IYAQIIDLTTREAVENRPEVHRRYIDIQFLAWGEEKIGIAIDTGNNKVSESLLEQRDI
                     IFYHDSEHESFIEMIPGSYAIFFPQDVHRPGCILQTASEIRKIVVKVALTALN"
BASE COUNT         4134 a         4127 c         4008 g         4004 t
ORIGIN      
        1 tatcccccac ccggtcaggc aggggataac gtttacgcca ttaatggcag aagttgctga
       61 tagaggcgac ggaacgtttc acgtcgtggc tgataagcgg cataacgctg cgcatctggt
      121 agatgcgact gttctaacgg tagctgcggc aacaactcaa tgagcgattt ctctggattc
      181 gccgcaatct gcgccagcct tgctgcgccc agtgctggcc ccacatcccc ccccgtacgg
      241 taatcgagct gctgaccgct gatatccgcc agcatctgac gccagtactc actacgcgcc
      301 ccgccaccaa tcaacgtaac actttgcggt ttaatgccgc aggcatgcac gacatccatg
      361 ccatctgcca gcgcataacc cacgccttcc agcactgctc gcgccagttc attggggcca
      421 tgttgatgag tcaaaccaaa gaaaaccccc ttcgcctggg gattattgtg tggtgtacgc
      481 tcgccggaaa gataaggcag aaaccaaact ggctcggcac tttcatcagc ctgttgagct
      541 gcagcgatta aagctgggac attgcacagg ccggttaatt tcgcgaccca atccagacac
      601 gatgctgcac tcagcattac agacattaaa tgccaacgct gcggtagcgc atggcaaaag
      661 ctgtgtacag cgctttctgg cttgcttaag aacccttcgc tgacagcaaa atagaccccc
      721 gacgtcccca gcgataacat tgcctgatta gcatcaacca ttcccacacc aactgcacca
      781 gctgcattgt cgccaccgcc tgcgacaact ggcaccgtcg ccatacccca cgctttcgca
      841 acttcaggta acaaagcacc agtaatttcg ctgccttcgt ataatgcggg catctggtca
      901 cgagataagt cgcaagcctg cagcatgacg tcactccagt cacgctttgc gacatccagc
      961 cacatggtgc cagctgcgtc agacatatcg ctggcaaact cccccgtcat acgcagacgc
     1021 aagtaatctt tcggtaataa tactttgtcg atttgacgga atatctccgg ctcatgccgc
     1081 tgaacccata gcaatttagg cgcagtaaat ccgggcatca tcaggttgcc ggtaatcact
     1141 cgtgattgcg gaactctcgc ttccagcaaa gtgcactctt gcgcacagcg cccgtcgttc
     1201 cacaaaatgg cagggcgcaa tacccgttgt tgagcatcca gtaaggttgc tccatgcatc
     1261 tggccggcaa tacccaatgc tttaacgtcc tgcagagaat gctgatcgcc cagagctttc
     1321 attgcgcgat cagttgcctg ccaccactgt tccgggtctt gttccgacca gagtggatgc
     1381 gggcgcgaaa cggtcagctt ttccgtttgc gaagcaacca cctcaccctg ctcgttgagc
     1441 aaaataactt ttacgcccga ggtgccaaga tctatcccga tatacatatc gatcgttcct
     1501 taaaaaaatg cccggtatcg ctaccgataa ccgggccaac ggactgcaca gttagccgtt
     1561 atttgtcgaa cagataatga tttaccagat tttccagttg ctcctggcga ccactctgat
     1621 gcaccggaga caaattatgt tcctgagcat atttggctaa atctgccagt gacatttggc
     1681 ctttcaggat ttgctggccc aattcgctat tccagccgga ataacgctgc gcgatgcgtt
     1741 tatccagctc gccatcttca atcatgcgcg ctgcaatttt cagcgccact gccatcgtat
     1801 ccatcgcgcc gatatgaccg taaaacagat catatttatc agtactttga cgacgtactt
     1861 tggcatcgaa gttcagacca ccggtggtga aaccacctgc tttgagaatt tcatacatca
     1921 ccagcgcatt ctcttccaca ctgttcggga actggtcggt gtcccagccc agttgcgcat
     1981 cgccacggtt ggcgtcgaca gaaccgaaca ggccaagcgc aatggcggtg gctatttcat
     2041 gatggaaaga gtgacctgcc agcgtcgcgt ggttagcttc aatgttcagt ttaatctctt
     2101 tttccagacc aaactgtttc aggaagccat agaccgtcgc ggcatcgtaa tcatattgat
     2161 gtttagtcgg ttcttgcggt ttcggttcga taagcaacgt gccctggaag ccgattttat
     2221 gtttatgctc aaccaccatc tgcataaagc ggcccagttg ttcacgctcc tgacgcaagt
     2281 cggtatttaa cagcgtttcg taaccttcac gaccacccca caggacatag ttttcaccgc
     2341 ccaatttatg ggttgcttcc atcgctgtaa caacttgcgt tgccgcccag ctgaagactt
     2401 caggatctgg gttcgtcgcc gcacccgcgc catagcgagg gtttgtaaag cagttagcgg
     2461 ttccccacag cagcttcacg ccgctctctt cttgcttggc tgccaggaca tcaaccattt
     2521 gcgcaaaatt attgatgtac tcttttaacg acgcgccctc aggggaaaca tccacatcgt
     2581 ggaagcaata aaatggcaca tgtaacttgt ggaaaaactc aaatgcgaca tctgctttac
     2641 gcttcgccaa cgccagtgcc tcaccaggct gctgccacgg acgattaaac gcccccacac
     2701 caaacatgtc cgccccgttc cagcagaagg tgtgccagta gcaggcggca aaacgcaagt
     2761 gctcttccat acgcttaccc aacaccagtt cgtcgggatt gtagtgacgg aatgctaacg
     2821 ggtttgagga ttttgagcct tcataacgaa cgcgatcgag ctggtcaaaa taggcttgca
     2881 tattgaactc cataatcagg taatgccgcg ggtgatggat gatgtcgtaa tattgggcac
     2941 tccctttcag ttgctcaatt atgttatttc acactgctat tgagataatt cacaagtgtg
     3001 cgctcgctcg caaaataaaa tgggatgatg aaactgggta attcctcgaa gataaaaatg
     3061 caataagtac aattgcgcaa caaaagtaag atctcggtca taaatcaaga aataaaccaa
     3121 aaatcgtaat cgaaagataa aaatctgtaa ttgttttccc ctgtttagtt gctaaaaatt
     3181 ggttacgttt atcgcggtga ttgttactta ttaaagctgt cctctaacta cagaaggccc
     3241 tacaccatga aaataaagaa cattctactc accctttgca cctcacttct gcttaccaac
     3301 gttgcggcac acgccaaaga agtcaaaata ggtatggcga ttgatgatct ccgtcttgaa
     3361 cgctggcaaa aagatcgaga tatttttgtg aaaaaggcag aatctctcgg cgcgaaagta
     3421 tttgtacagt ctgcaaatgg caatgaagaa acacaaatgt cgcagattga aaacatgata
     3481 aaccggggtg ttgatgttct tgtcattatt ccgtataacg gtcaggtatt aagtaacgtt
     3541 gtaaaagaag ccaaacaaga aggtattaaa gtattagctt acgatcgtat gattaacgat
     3601 gcggatatcg atttttatat ttctttcgat aacgaaaaag tcggtgaact gcaggcaaaa
     3661 gccctggtcg atattgttcc gcaaggtaat tacttcctga tgggcggctc gccggtagat
     3721 aacaacgcca agctgttccg cgccggacaa atgaaagtgt taaaacctta cgttgattcc
     3781 ggaaaaatta aagtcgttgg tgaccaatgg gttgatggct ggttaccgga aaacgcattg
     3841 aaaattatgg aaaacgcgct aaccgccaat aataacaaaa ttgatgctgt agttgcctca
     3901 aacgatgcca ccgcaggtgg ggcaattcag gcattaagcg cgcaaggttt atcagggaaa
     3961 gtagcaattt ccggccagga tgcggatctc gcaggtatta aacgtattgc tgccggtacg
     4021 caaactatga cggtgtataa acctattacg ttgttggcaa atactgccgc agaaattgcc
     4081 gttgagttgg gcaatggtca ggaaccaaaa gcagatacca cactgaataa tggcctgaaa
     4141 gatgtgccct cccgcctcct gacaccgatc gatgtgaata aaaacaacat caaagacacg
     4201 gtagttaaag acggattcca caaagagagc gagctgtaag cgttacgccc cggcgcggag
     4261 cgggggcgtg atttctctcc atgccgcgtg aatgaattgg cttaggtgga gtcgttatgc
     4321 cttatctact tgaaatgaag aatattacca aaaccttcgg cagtgtgaag gcgattgata
     4381 acgtctgctt gcggttgaat gctgacgaaa tcgtctcact ttgtggggaa aatgggtctg
     4441 gtaaatcaac gctgatgaaa gtgctgtgtg gtatttatcc ccatggctcc tacgaaggcg
     4501 aaattatttt tgcgggagaa gagattcagg cgagtcacat ccgcgatacc gaacgcaaag
     4561 gtatcgccat cattcaccag gaattggccc tggtgaaaga attgaccgtg ctggaaaata
     4621 tcttcctggg taacgaaata acccacaatg gcattatgga ttatgacctg atgacgctac
     4681 gctgtcagaa gctgctcgca caggtcagtt tatccatttc acctgatacc cgcgttggcg
     4741 atttagggct tgggcaacaa caactggttg aaattgccaa ggcacttaat aaacaggtgc
     4801 gcttgttaat tctcgatgaa ccgacagcct cattaactga gcaggaaacg tcggttttac
     4861 tggatattat tcgcgatcta caacagcacg gtatcgcctg tatttatatt tcgcacaaac
     4921 tcaacgaagt caaagcgatt tccgatacga tttgcgttat tcgcgacgga cagcacattg
     4981 gtacgcgtga tgctgccgga atgagtgaag acgatattat caccatgatg gtcgggcgag
     5041 agttaaccgc gctttaccct aatgaaccac ataccaccgg agatgaaata ttacgtattg
     5101 aacatctgac ggcatggcat ccggtcaatc gtcatattaa acgagttaat gatgtctcgt
     5161 tttccctgaa acgtggcgaa atactgggta ttgccggact cgttggtgcc ggacgtaccg
     5221 agaccattca gtgcctgttt ggcgtgtggc ccggacaatg ggaaggaaaa atttatattg
     5281 atggcaaaca ggtagatatt cgtaactgtc agcaagccat cgcccagggg attgcgatgg
     5341 tacccgaaga cagaaagcgc gacggcatcg ttccggtaat ggcggttggt aaaaatatta
     5401 ccctcgccgc actcaataaa tttaccggtg gcattagcca gcttgatgac gcggaagagc
     5461 aaaaatgtat tctggaatca attcagcaac tcaaagttaa aacgtcgtcc cccgatcttg
     5521 ctattggacg tttgagcggc ggcaatcagc aaaaagcgat cctcgctcgc tgtctgttac
     5581 ttaacccgcg catcctcatt cttgatgaac ctaccagggg tatcgatatt ggcgcgaaat
     5641 acgagatcta caaattaatt aaccaactcg tccagcaggg tattgccgtt attgtcatct
     5701 cttccgaatt acctgaagtg ctcggcctta gcgatcgggt actggtgatg catgaaggga
     5761 aactaaaagc caacctgata aatcataacc tgactcagga gcaggtgatg gaagccgcat
     5821 tgaggagcga acatcatgtc gaaaagcaat ccgtctgaag tgaaattggc cgtaccgaca
     5881 tccggtggct tttccgggct gaaatcactg aatttgcagg tcttcgtgat gattgcagct
     5941 atcatcgcaa tcatgctgtt ctttacctgg accaccgatg gtgcctactt aagcgcccgt
     6001 aacgtctcca acctgttacg ccagaccgcg attaccggca tcctcgcggt aggaatggtg
     6061 ttcgtcataa tttctgctga aatcgacctt tccgtcggct caatgatggg gctattaggt
     6121 ggcgtcgcgg cgatttgtga cgtctggtta ggctggcctt tgccacttac catcattgtg
     6181 acgttggttc tgggactgct tctcggtgcc tggaacggat ggtgggtcgc gtaccgcaaa
     6241 gtcccttcat ttattgtcac cctcgcgggc atgttggcat ttcgcggcat actcattggc
     6301 atcaccaacg gcacgactgt ttcccccacc agcgccgcga tgtcacaaat tgggcaaagc
     6361 tatctacctg ccagcactgg cttcatcatt ggcgcgcttg gcttaatggc ttttgttggt
     6421 tggcaatggc gcggaagaat gcgccgtcag gctttgggtt tgcagtctcc ggcctctacc
     6481 gcagtagtcg gtcgccaggc tttaaccgct atcatcgtat taggcgcaat ctggctgttg
     6541 aatgattacc gtggcgttcc cactcctgtt ctgctgctga cattgctgtt actcggcgga
     6601 atgtttatgg caacgcggac ggcatttgga cgacgcattt atgccatcgg cggcaatctg
     6661 gaagcagcac gtctctccgg gattaacgtt gaacgcacca aacttgccgt gttcgcgatt
     6721 aacggattaa tggtagccat cgccggatta atccttagtt ctcgacttgg cgctggttca
     6781 ccttctgcgg gaaatatcgc cgaactggac gcaattgcag catgcgtgat tggcggcacc
     6841 agcctggctg gcggtgtggg aagcgttgcc ggagcagtaa tgggggcatt tatcatggct
     6901 tcactggata acggcatgag tatgatggat gtaccgacct tctggcagta tatcgttaaa
     6961 ggtgcgattc tgttgctggc agtatggatg gactccgcaa ccaaacgccg ttcttgattt
     7021 tgataaaaat tttctcaaag ccggttacgt attaccggtt ttgagttttt gcatgattca
     7081 gcaggaaaag aaccatgttt actaaacgtc accgcatcac attactgttc aatgccaata
     7141 aagcctatga ccggcaggta gtagaaggcg taggggaata tttacaggca tcacaatcag
     7201 aatgggatat tttcattgaa gaagatttcc gcgcccgcat tgataaaatc aaggactggt
     7261 taggagatgg cgtcattgcc gacttcgacg acaaacagat cgagcaagcg ctggctgatg
     7321 tcgacgtccc cattgttggg gttggcggct cgtatcacct tgcagaaagt tacccacccg
     7381 ttcattacat tgccaccgat aactatgcgc tggttgaaag cgcatttttg catttaaaag
     7441 agaaaggcgt taaccgcttt gctttttatg gtcttccgga atcaagcggc aaacgttggg
     7501 ccactgaacg cgaatatgca tttcgtcagc ttgtcgccga agaaaagtat cgcggagtgg
     7561 tttatcaggg gttagaaacc gcgccagaga actggcaaca tgcgcaaaat cggctggcag
     7621 actggctaca aacgctgcca ccgcaaaccg ggattattgc cgttactgac gcccgggcgc
     7681 ggcatattct gcaagtatgt gaacatctac atattcccgt accggaaaaa ttatgcgtga
     7741 ttggcatcga taacgaagaa ctgacccgct atctgtcgcg tgtcgccctt tcttcggtcg
     7801 ctcagggcgc gcggcaaatg ggctatcagg cggcaaaact gttgcatcga ttattagata
     7861 aagaagaaat gccgctacag cgaattttgg tcccaccagt tcgcgtcatt gaacggcgct
     7921 caacagatta tcgctcgctg accgatcccg ccgttattca ggccatgcat tacattcgta
     7981 atcacgcctg taaagggatt aaagtggatc aggtactgga tgcggtcggg atctcgcgct
     8041 ccaatcttga gaagcgtttt aaagaagagg tgggtgaaac catccatgca atgattcatg
     8101 ccgagaagct ggagaaagcg cgcagtctgc tgatttcaac caccttgtcg atcaatgaga
     8161 tatcgcaaat gtgcggttat ccatcgctgc aatatttcta ctctgttttt aaaaaagcat
     8221 atgacacaac gccaaaagag tatcgcgatg taaatagcga ggtcatgttg tagcgatgat
     8281 gagaattgtc ggcgtcacat cagggaactc agcacagcca cctgatgcga gcaaaagcct
     8341 tatccgaccg acaaatcaga taaggctcaa ggaaatgcaa ttacatatgc gccgcgatta
     8401 accgttggtt atcctggtac attgcgaaca ggtagttgtt ataactcttc cctttggtcg
     8461 agtagccctt cagcttgtga atcatcgctg tggcagtcac ttcctgatcc gctttacgca
     8521 gctgcgcacg cgatttacgg aatgaagagt aagccggatg cgtattgagg ttagtgacat
     8581 aggcgctcac cgattctttg acagaactaa actgtgagta ccctttcact ttacccggcg
     8641 cattggtaca acgtcctttc atgcatttca tgccgaacag attgttgttg ttgcgcgcca
     8701 gctttgacgt tccccaaccg ctttctgctg cagccatcgt cgccaccata ctggtgggga
     8761 taatgtctac gcgttcaagc aaggtattcc acgggatttt tcgcgtatta ccagaccact
     8821 tcaccttgta gcgtttggcg atgtctttca gacgcgcacg ctcagcaggt gaccattggc
     8881 cctgatactg ttttgaaatc agccagttac gttccgcagt aatggctgca ttttggctgg
     8941 taatgtaagg cattacggtc cggagaaacg cctttttcct tggtgttccg gaagggtatt
     9001 ttcgcaaatc aggaagtgaa ttactctttg cactattgcg agaatactct tgtttactgc
     9061 ttacctgttt attactagct ttagttaagt gggacttttg actcgctgtt gttgtgtgcg
     9121 tcttcgctaa cacctcactc gaaaacacca gagtgagtaa cataagaatc atcgccccat
     9181 atcgtcgtat gggagtcaaa atcatcaggt ctcctggtcg gatttaatca ttccaacacc
     9241 ttatattttt cacaaatttg agagttgaat ctcaaatcat atcaaaaata gctgtcaaga
     9301 gcaccccaag gaatagtcca aatctgaaac tatgtcacga gttaacgatt cagattggcg
     9361 ctaaatcgca gaaaatgtgg ggtttatcgc aaaattcagc cgttttttgc gcgagatcgc
     9421 tcacccttgc ttctcatcct gtggacttac cgctcaggga tgagttttgt ttggcttatc
     9481 gctggcaaac tgtctgaaat cgcagcaata aggactcatc cgccatgaaa ctcgccgcct
     9541 gttttctgac actccttcct ggcttcgccg ttgccgccag ctggacttct ccggggttcc
     9601 ctgcctttag tgaacaggga acggaaacat ttgtcagcca cgcgcagttg cccaaaggta
     9661 cgcgtccact aacgctaaat tttgaccaac agtgctggca gcctgcggat gcgataaaac
     9721 tcaatcagat gctttccctg caaccttgta gcaacacgca gcctcaatgg cgattgttca
     9781 gggacggcga atatacgctg caaatagaca cccgctccgg cacgccaaca ttgatgattt
     9841 ccatccagaa cgccgccgaa ccggtagcaa gccaggtccg tgtatgcccg aaatgggatg
     9901 gattaccgct cacgctggat gtcagcgcca ctttcccgga aggagccgcc gtacgggatt
     9961 attacagcca gcaaattgcg atagtgaaga acggtcaaat aacgttacaa cccgctgcca
    10021 ccagcaacgg tttactcctg ctggaacggg cagaaactga cgcctctgcc cctttcgact
    10081 ggcataacgc cacggtttac tttgtgctga cagatcgttt cgaaaacggc gatcccagta
    10141 atgaccagag ttacggacgt cataaagacg gtatggcgga aattggcact tttcacggcg
    10201 gcgatttacg cggcctgacc aacaaactgg attacctcca gcagttgggc gttaatgctt
    10261 tatggataag cgccccattt gagcaaattc acggctgggt cggcggcggt acaaaaggcg
    10321 atttcccgca ttatgcctac cacggttatt acacacagga ctggacgaat cttgatgcca
    10381 atatgggcaa cgaagccgat ctacggacgc tggttgatag cgcacatcag cgcggtattc
    10441 gtattctctt tgatgtcgtg atgaaccaca ccggctatgc cacgctggcg gatatgcagg
    10501 agtatcagtt tggcgcgtta tatctttctg gtgacgaggt gaaaaaaacg ctgggtgaac
    10561 gctggagcga ctggaaacct gccgccgggc aaacctggca tagctttaac gattacatta
    10621 atttcagcga caaaacaggc tgggataaat ggtggggaaa aaactggatc cgtaccgata
    10681 tcggcgatta cgacaatcct ggattcgacg atctcaccat gtcgctagcc tttttgccgg
    10741 atatcaaaac cgaatcaact accgcttctg gtctgccggt gttctataaa aacaaaacgg
    10801 atacccacgc taaagccatc gacggcttta cccctcgcga ttacttaacc cactggttaa
    10861 gtcagtgggt ccgcgactat gggattgatg gttttcgggt cgataccgcc aaacatgttg
    10921 agttgcccgc ctggcagcaa ctgaaaaccg aagccagcgc cgcgcttcgc gaatggaaaa
    10981 aagctaaccc cgacaaagca ttagatgaca aacctttctg gatgaccggt gaagcctggg
    11041 gccacggcgt gatgcaaagt gactactatc gccacggctt cgatgcgatg atcaatttcg
    11101 attatcagga gcaggcggcg aaagctgtcg attgtattgc gcagatggat atgacctggc
    11161 agcaaatggc ggagaaattg cagggtttca acgtgttgag ctacctctcg tcgcatgata
    11221 cccgtctgtt ccgtgaaggg ggcgacaaag cagcagagtt attactatta gcgccaggcg
    11281 cggtacaaat cttttatggc gatgaatcct cgcgtccgtt cggtcctaca ggttctgatc
    11341 cgctgcaagg tacacgttcg gatatgaact ggcaggatgt tagcggtaaa tctgccgcca
    11401 acgtcgcgca ctggcagaaa atcagccagt tccgcgcccg ccatcccgca attggcgcgg
    11461 gcaaacaaac gacactttcg ctgaagcagg gctacggctt tgttcgtgag catggcgacg
    11521 ataaagtgct ggtcatctgg gctgggcaac agtgactttt ccggcttccc gttcgtcagt
    11581 acctcgggaa gccgccaacc aggataaaat gtcagcccta atcagcgttg caggataaag
    11641 caccgctcac tcttcaacag accgatttgc accccagcaa atgtagcgtt attgttacct
    11701 tccttgctac agagttcgac agatatcccg ctatgacatt ctcccttttt ggtgacaaat
    11761 ttacccgcca ctccggcatt acgctgctga tggaagatct gaacgacggt ttacgcaccc
    11821 ctggcgcgat tatgctcggc ggcggtaatc cggcgcagat cccggaaatg caggactact
    11881 tccagacgct actgaccgac atgctggaaa gtggcaaagc gactgatgca ctgtgcaatt
    11941 acgacggtcc acaggggaaa acggagctac tcacactgct tgccggaatg ctgcgcgaga
    12001 agttgggttg ggatatcgaa ccacagaata ttgcactaac aaacggcagc cagagcgcgt
    12061 ttttctactt atttaatctg tttgccggac gccgtgccga tggtcgggtc aaaaaagtgc
    12121 tgttcccgct ggcaccggaa tacattggct acgctgacgc cggactggaa gaagatctgt
    12181 ttgtctctgc gcgtccgaat attgaactgc tgccggaagg ccagtttaaa taccacgtcg
    12241 attttgagca tctgcatatt ggcgaagaaa ccgggatgat ttgcgtctcc cggccgacga
    12301 atccaacagg caatgtgatt actgacgaag agttgctgaa gcttgacgcg ctggcgaatc
    12361 aacacggcat tccgctggtg attgataacg cttatggcgt cccgttcccg ggtatcatct
    12421 tcagtgaagc gcgcccgcta tggaatccga atatcgtgct gtgcatgagt ctttccaagc
    12481 tggggctgcc tggctcccgc tgcggcatta tcatcgccaa tgaaaaaatc atcaccgcca
    12541 tcaccaatat gaacggcatt atcagcctgg cacctggcgg tattggtccg gcgatgatgt
    12601 gtgaaatgat taagcgtaac gatctgctgc gcctgtctga aacagtcatc aaaccgtttt
    12661 actaccagcg tgttcaggaa actatcgcca tcattcgccg ctatttaccg gaaaatcgct
    12721 gcctgattca taaaccggaa ggagccattt tcctctggct atggtttaag gatttgccca
    12781 ttacgaccga gcagctctat cagcgcctga aagcacgcgg cgtgctgatg gtgccggggc
    12841 acaacttctt cccagggctg gataaaccgt ggccgcatac gcatcaatgt atgcgcatga
    12901 actacgtacc agagccggag aaaattgagg cgggggtgaa gattctggcg gaagagatag
    12961 aaagagcctg ggctgaaagt cactaaaaat tgcctgatgc gctacgctta tcaggcctac
    13021 atgatttctg caatatattg aatttggaag aatttgtagg ccggataagg cgtttacgcc
    13081 gcatctggca taaacaaagc gcactattcc ggcgttgaga aacgccggaa aaacgtttca
    13141 aacaggctgc tgccgtagcc gatgccgctg cactttctcg acatccatgc actgcaacgc
    13201 ctttgtcggg caggcttcaa cacaggccgg tcccgtctcc cgatgccagc acagatcgca
    13261 cttaatcgcc ctcgcctttt tgcgcgaaga aacgacctcc atcgcaccaa acgggcaagc
    13321 cagcatacag cttttacagc caatgcaacg tgtttgttca acgaaaatat gcccatgttc
    13381 gcggcttatc gcgtcaacag ggcagacatt cgcgcacggt gcatcttcac actgatgaca
    13441 ggctactgcc gtggtccagc tgtggtcttt aatgacacga atacgggaaa taaactcgtc
    13501 tggtgacaac gcagcgcaat cctgattctc ctgatgcgac actgcgcaag ccacttcaca
    13561 ggtacggcaa ccgatacatt tcgtcgcatc cgcaataata aaccggttca tgatcactcc
    13621 tttgcactaa aacagcagag tgccagagag cgcgtcccta ctgctttgat ccaacgaagg
    13681 aaatgttaaa gttttggcac ttgtccaaag cgttatgtga tcgcgcccag gtcatccctg
    13741 acagtaaatc ccagttcatt agaaatagcc tgcgcggttt cacgcagtgg tttcaggaga
    13801 tttttctctc ccacctgttt cagacgtgat gtcgaaagcg aaatcgacac ggcgtacggc
    13861 acccgcccat gaatatcaaa caccggaaca gcaatacagg agacgccgag ttcgttttct
    13921 tctctgtcca tcgccgctcc gctttcacga atgtgcgcca gttcgtcgaa catcgcgggc
    13981 agctcggtaa tggtattgcg ggttaacggc tggatctcat gctgatggtt ttcccagtat
    14041 gacttcacgt agtccgggtg accaaacgcc atgtagatct tgcccattgc ggaacagtag
    14101 agcggcatat gctggccaat ataggcacgg gttcgcagca tcccggttgt gggttccagc
    14161 ttataaatca aaatagcgtg atcgtcttcg cggctggaga agttaatggt ttcaccagtg
    14221 gcgatgttca gtgcctcaag atgcggagcg gcgatatgaa tgatattcag cgaagacagc
    14281 gccttctgcc cgacggcaat aaatttggta gtcaggcggt aactccctgc ggcgggcgcg
    14341 gtggtcacat agccgcagga ctgtaatccc tgcaataagc gatggacggt actcttattt
    14401 aaaccagcca gctccgaaag atgcgccaac ggacaaccgt ttggatagtt gctcaaaatc
    14461 tcaatcagca tcaacccgcg aaacagactc tggcttccgg ctggacgctc tttttcctgc
    14521 gccatctcgt tctctttttt tcccatcact tctttcccca ttttgtcgcg tcctgatggt
    14581 agcgcaaagt gtgccgtagt tcacgatctc gacagataat ttataaccac ttgattttta
    14641 tgtcttttga aattcatcaa tcagattgct ttgttaaaaa gtgatcgata tatttgaaat
    14701 caagtttcgc atattgaaat tttaagccaa aaaagcgatc aaaaaaacaa ggaagcctgg
    14761 gatgaaagtg acatttgagc agttaaaagc agcctttaat cgggtcttaa tttcacgcgg
    14821 cgttgacagc gaaacggctg acgcctgtgc agagatgttc gcccgcacca ccgaatccgg
    14881 cgtttattct cacggcgtta atcgtttccc tcgtttcatt caacaactgg aaaacggcga
    14941 tatcattcct gatgcccaac ccaaacgtat aaccagcctc ggcgcaattg aacagtggga
    15001 tgcccagcgt tcgatcggca acctgacggc gaaaaagatg atggatcgcg ccattgaact
    15061 ggctgccgat cacggtattg gtctggtggc acttcgtaat gccaaccact ggatgcgcgg
    15121 cggcagctac ggctggcagg cggcggaaaa aggctatatt ggcatttgct ggaccaactc
    15181 catcgccgta atgccgccgt ggggcgcaaa agagtgtcgc attggcacta acccgctgat
    15241 cgtcgccatt ccttccacgc cgatcaccat ggtcgatatg tcgatgtcga tgttctctta
    15301 cggcatgtta gaagttaacc gcctggcggg ccgtcagctc ccggtcgatg gtggctttga
    15361 tgatgagggc aatttgacca aagaacctgg cgttatcgag aagaatcgcc gcattttgcc
    15421 gatgggctac tggaaaggtt ctggcatgtc gattgtgctg gatatgatcg ctactctcct
    15481 ttccgacggc gcatcggttg ccgaagtcac ccaggacaac agcgacgaat acggcatttc
    15541 acaaattttt attgccattg aagtggacaa gcttatcgac ggtcccaccc gcgatgccaa
    15601 gctgcaacgt atcatggatt acgttactac cgctgaacgc gctgacgaaa accaggccat
    15661 ccgcttaccc ggccacgaat ttactaccct gctggccgaa aaccgccgta acggtattac
    15721 cgttgatgac agcgtgtggg ccaaaatcca ggcgttataa ggagataagt catgattttt
    15781 ggacatatcg cgcagcccaa tccgtgccgt ttgcccgccg ccattgaaaa ggcgctcgat
    15841 tttctgcgcg ctaccgattt caacgccctg gagccgggcg ttgtcgaaat cgacggcaaa
    15901 aatatttatg cgcaaattat cgatttaacc acacgcgaag cggtggaaaa ccgtccggaa
    15961 gtgcatcgtc ggtatatcga tattcagttt ctggcgtggg gtgaagagaa aatcggcatt
    16021 gctattgata cgggaaataa taaagtcagc gaatcactat tagagcagcg cgatattatt
    16081 ttttatcatg acagtgaaca tgaatcattt atagaaatga taccgggcag ctacgcaatt
    16141 ttctttccgc aggatgttca ccgaccaggt tgtattttgc aaacagcctc tgagatacga
    16201 aaaattgtgg ttaaagtcgc attaacggcg ctgaattaat cgaataatcg tctacgccag
    16261 aacgactggg taa
//
LOCUS       DAZKHF020000085        16151 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2201S31241
            SAMN43267800-rid25106413.denovo.086, whole genome shotgun sequence.
ACCESSION   DAZKHF020000085 DAZKHF020000000
VERSION     DAZKHF020000085.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267800
            Sequence Read Archive: SRR30307263
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 16151)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16151)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 16151)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350581.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350581.2
            Genome Coverage       :: 151x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:21:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,652
            CDSs (total)                      :: 5,557
            Genes (coding)                    :: 5,320
            CDSs (with protein)               :: 5,320
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 237
            CDSs (without protein)            :: 237
            Pseudo Genes (ambiguous residues) :: 0 of 237
            Pseudo Genes (frameshifted)       :: 102 of 237
            Pseudo Genes (incomplete)         :: 130 of 237
            Pseudo Genes (internal stop)      :: 52 of 237
            Pseudo Genes (multiple problems)  :: 41 of 237
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16151
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267800-rid25106413.denovo.086"
                     /serotype="O 26:H11"
                     /isolate="2201S31241"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-01"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            5..544
                     /locus_tag="ACHOSC_RS11460"
                     /old_locus_tag="pgaptmp_002292"
     CDS             5..544
                     /locus_tag="ACHOSC_RS11460"
                     /old_locus_tag="pgaptmp_002292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000421825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1441 family protein"
                     /protein_id="HGU4548222.1"
                     /translation="MELLMSNVSGIGDAYYWSVFKIAEAFGLHRDTVKKRLLAANTPV
                     AATVRGNPVYALQHVGPALFSVKHEAADSVHDPSRMEPKERKDWYQSENERIKLEKEQ
                     RKLIPVDEVVIVYSSMRKAVVQVLETIPDVLERDCALTPQAVGVVQQAIDDLRYTLQE
                     KSYEACAAELIPDEEGESL"
     gene            553..2652
                     /locus_tag="ACHOSC_RS11465"
                     /old_locus_tag="pgaptmp_002293"
     CDS             553..2652
                     /locus_tag="ACHOSC_RS11465"
                     /old_locus_tag="pgaptmp_002293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015987600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage terminase large subunit family protein"
                     /protein_id="HGU4548223.1"
                     /translation="MGFSSARNLGRDISAGFSPPRRMPISEAVKKFMRVPKGAGNSVP
                     WDPELTPYIIEPMNCLASREYDAVIFVGPARTGKTIGLIDGWIVYTIVCDPSDMLVVQ
                     MTEDKAREHSKKRLDRTFRSSAAVKKRMSPRRNDNNVHDKTFRDGSFLKIGWPSVNIM
                     SSSDYRFVALTDYDRFPENIDSEGDGFSLASKRTTTFMSAGMTLVESSPGRDICDSKW
                     RRKSPHEAPPTTGILSLYNRGDRRRWYWSCPHCGEYFQPAMDAMTGYRNEPDPFKASE
                     AAYLLCPHCSGIITAEKKRELNSAGVWLREGQVIDRNGNVSGEPRRSRIASFWMEGPA
                     AAYQTWAQLVYKLLTAEQEYEATGSEETLRAVINTDWGLPYLPRASMEQRKSELLEQR
                     AEPVPSRSVPDGVNFLVATVDVQAGRHRRFVVQVTGYGSRGERWIIDRYNITQSLRSD
                     CDGESQRIDPASYPEDWDVLLTDVFHKSWPLASDPSQQMRLMAMAVDSGGEDGVTDNA
                     YKFWRRCRRDGLGKRIYLFKGDSIRRAKLISRTFPDNTGRTGRRAQAAGDVPLWLLQT
                     DALKDRVNNALWRDSPGPGYVHFPDWLGSWFYDELTYEERSSDGKWSKPGRGANEAFD
                     LMVYAEALVILHGYEKIRWPDAPEWASRETWLECVQDSTEPSSSPEPVSTPVKKQKRK
                     KTVTDDVNPWLTSGGWL"
     gene            2649..2861
                     /locus_tag="ACHOSC_RS11470"
                     /old_locus_tag="pgaptmp_002294"
     CDS             2649..2861
                     /locus_tag="ACHOSC_RS11470"
                     /old_locus_tag="pgaptmp_002294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001072975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4548224.1"
                     /translation="MNQNDIEAMIQRYTEAEMAVLDGKSVTFNGQQMTMENLSEIRQG
                     RQEWERRLAALITRRRGHPGYRLARF"
     gene            2861..4369
                     /locus_tag="ACHOSC_RS11475"
                     /old_locus_tag="pgaptmp_002295"
     CDS             2861..4369
                     /locus_tag="ACHOSC_RS11475"
                     /old_locus_tag="pgaptmp_002295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HGU4548225.1"
                     /translation="MAILDDVIGVFSPGWKAARLRSRAVIQAYEAVKTTRTHKARREN
                     RTADQLSQYGAVSLREQARYLDNNHDLVIGVFDKLEERVVGKNGIIVEPHPVLRNGAI
                     ARDLAAEIRTRWSEWSVSPEVTGQFTRPMLERLMLRTWLRDGEVFAQMVSGRINSLAP
                     SAGVHFWLEALEPDFIPMTSDESNRLNQGVFVDDWGRPEKYLVYKSRPVSGRQMETKE
                     VDAERMLHLKFVRRLHQMRGTSLLSGVLIRLSALKEYEDSELTAARIAAALGMYIRKG
                     DGQSYEPDGNGRKENERELTIQPGIIYDDLKPGEEIGMVKSDRPNPNLETFRNGQLRA
                     VAAGSRLSFSSTARNYNGTYSAQRQELVESTDGYLILQDWFIGAVTRPMYRAWLKQAV
                     ASGVIRLPRDLDRSSLYTAVYSGPVMPWIDPVKEAEAWKIQIRGGAATESDWVRAGGR
                     NPDDVKRRRKAEIDENRKLDLVFDTDPASDKGGSSAATKRQEPQHTDDQSEE"
     gene            4314..6341
                     /locus_tag="ACHOSC_RS11480"
                     /old_locus_tag="pgaptmp_002296"
     CDS             4314..6341
                     /locus_tag="ACHOSC_RS11480"
                     /old_locus_tag="pgaptmp_002296"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308856.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0019082 - viral protein processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ClpP-like prohead protease/major capsid protein
                     fusion protein"
                     /protein_id="HGU4548226.1"
                     /translation="MPQRNDRSRSTPTTSPKNNSWFRMQAGHQSDADIYIYDEIGFWG
                     VTAKQFISDLNALGDITHINLHINSPGGDVFEGIAIFNALKTHGASITVYVDGVAASM
                     ASVIAMVGNPVIMPENSFMMIHKPFGFTGGDAEDMRTYADLLDKVEAVLLPAYAQKTG
                     KTTDEIAAMLADETWMSGAECLAQGFADQVTPAVKAMACIQSKRTEEFKKMPESIRNM
                     ITPPRNSAPRVQDDGPAASRTPVQAAAPVVDENSIRAQVLAEQKARVNGINDLFAMFG
                     GRYQSLQAQCLADPECSLEQAREKLLNEMGRESTPSNKNTPAHIYAGNGNFVGDGIRQ
                     ALMARAGFEKTERDNVYNGMTLREYARMSLTERGIGVSSYNPMQMVGAAFTHSTSDFG
                     NILLDVANKAILQGWEDAPETYEQWTRKGQLSDFKIAHRVGMGGFSALRQVREGAEYK
                     YVTTGDKQATIALATYGELFSITRQAIINDDLNMLTDVPMKLGRAAKSTIADLVYAIL
                     TSNPKISTDNVSLFDKAKHANVLESAAMDVASLDKARQLMRVQKEGERHLNIRPAFVL
                     VPTAMESVANQVIRSSSVKGADINAGIINPVKDFATVIAEPRLDDNSQTTFYLAASKG
                     SDTIEVAYLNGVDTPYIDQMEGFSVDGVTTKVRIDAGVAPVDHRGLVKCTA"
     gene            6428..6751
                     /locus_tag="ACHOSC_RS11485"
                     /old_locus_tag="pgaptmp_002297"
     CDS             6428..6751
                     /locus_tag="ACHOSC_RS11485"
                     /old_locus_tag="pgaptmp_002297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308857.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2190 family protein"
                     /protein_id="HGU4548227.1"
                     /translation="MAKNFVEEGKTVAIVASAAISSGDLVQVGDVFAVALTDIPQGET
                     GDGITEGVFMLPKLKTDDMKTGKKVYLKSGKVQLTNSGSEPLVGVVWADAGTSAEEVP
                     VKLNV"
     gene            6744..7019
                     /locus_tag="ACHOSC_RS11490"
                     /old_locus_tag="pgaptmp_002298"
     CDS             6744..7019
                     /locus_tag="ACHOSC_RS11490"
                     /old_locus_tag="pgaptmp_002298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA breaking-rejoining protein"
                     /protein_id="HGU4548228.1"
                     /translation="MSDPFSRLAARMDAITVRKMGKTASINDVDMIVIPGETLAELNA
                     LSGPAVSLVVFSSGYRPRRGDRVVYDGQQWTVTRHERFNGKPMIFIE"
     gene            7031..7609
                     /locus_tag="ACHOSC_RS11495"
                     /old_locus_tag="pgaptmp_002299"
     CDS             7031..7609
                     /locus_tag="ACHOSC_RS11495"
                     /old_locus_tag="pgaptmp_002299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HGU4548229.1"
                     /translation="MKGLENAIRNLNSLDTRMVPQASAWAINRVAQKAVSVATRQVAG
                     NTVAGDNQVKGIPLKLVRQRVRVFKASPSGKMTVRIRVNRGNLPAIKLGTARVRLARR
                     GGKLQYRGSVLKVGKYLFRDAFIQQLANGRWHVMRRIDGKNRYPIDVVKIPLSGSLTQ
                     AFEDARDHIIAAEMPKQLGYALKQQLRLWLTR"
     gene            7606..8007
                     /locus_tag="ACHOSC_RS11500"
                     /old_locus_tag="pgaptmp_002300"
     CDS             7606..8007
                     /locus_tag="ACHOSC_RS11500"
                     /old_locus_tag="pgaptmp_002300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage minor tail U family protein"
                     /protein_id="HGU4548230.1"
                     /translation="MNRHTQIRQAVLARLREQCGDSATFFDGLPAFIDAQELPAVAVW
                     LSDAQYTGKMTDEDDWQAVLHIAVFIRAQAPDSELDMWMESTIFPALNDIPALSGLID
                     TLIPLGFNYQRDNEMATWAMAEITYQITYTN"
     gene            8018..8761
                     /locus_tag="ACHOSC_RS11505"
                     /old_locus_tag="pgaptmp_002301"
     CDS             8018..8761
                     /locus_tag="ACHOSC_RS11505"
                     /old_locus_tag="pgaptmp_002301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HGU4548231.1"
                     /translation="MTTPNPLAKTKGAGTTFWMYTGNGDAFANPLSDTDWLRLAMVKD
                     LQPGEMTADAEDDTYLDDEDADWKTTTQGQKSVGDTSATLAWRPGDSGQKKLVQLFDS
                     GEVCAFRIKYPNGTVDVFRGWLSSLGKTIASKDVMTRTVKISGVGRPYLAEEGTETVS
                     VTGLTVSPASASVKVGATTTLTFTVKPDGASDKAISVHSSDPQTATVTLNGLVATVKG
                     VKQGSVSIVGMTADGNFVAVTAVTVSAAG"
     gene            8822..9208
                     /locus_tag="ACHOSC_RS11510"
                     /old_locus_tag="pgaptmp_002302"
     CDS             8822..9208
                     /locus_tag="ACHOSC_RS11510"
                     /old_locus_tag="pgaptmp_002302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage minor tail protein G"
                     /protein_id="HGU4548232.1"
                     /translation="MFLKQDTFNYEKQSVVLSELSGLQRIEYLTFVQQRTAKFDAGEG
                     ELPEAERQIAFLRMGMDINAWLVSRSLWNADQSKDVETLCASVITTWSYDALGAGAEM
                     VLSLSGMGAIDNAVDDEHEALTPEKS"
     gene            9217..9546
                     /locus_tag="ACHOSC_RS11515"
                     /old_locus_tag="pgaptmp_002303"
     CDS             9217..9546
                     /locus_tag="ACHOSC_RS11515"
                     /old_locus_tag="pgaptmp_002303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail assembly protein T"
                     /protein_id="HGU4548233.1"
                     /translation="MQFVMRLAREFRRADWRRMLSEMSATELGEWGDYFRMQSFSDVW
                     MDAQFASLKALIVRMVSGSSDAAVADFSLLPEENGIPERTDEELMHLGEGISGGVRYG
                     PDSQPGH"
     gene            9518..12583
                     /locus_tag="ACHOSC_RS11520"
                     /old_locus_tag="pgaptmp_002304"
     CDS             9518..12583
                     /locus_tag="ACHOSC_RS11520"
                     /old_locus_tag="pgaptmp_002304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016032 - viral process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tape measure protein"
                     /protein_id="HGU4548234.1"
                     /translation="MDQIANLVIDLGIDAAEFKNEIPRIKNLLNGAASDAERSSARMQ
                     RFMERQTQAARQTTQAASSAATAASVHAQTVEKNAQAHERMAREVEKTRQRMEALSQK
                     MREEQAQAMALAEAQDKAAAAFYRQIDSVKQASAGLQELQRIQQQIRQARNSGGIGQQ
                     DYLALISEVTAKTRVLTQAEEEATRQKVAFIRQLKEQATRLNLSSSELLRAKAAQLGV
                     SSAAEVYIRKMEQAGKATHSLGLKSAAARQEIGVLIGELARGNLGALRGSGITLANRA
                     GWIDTLMSPKGMMLGGVIGGIAAAVYGLGKAWYDGQKEGEEFNRQLSLTGHYAGVTAG
                     QLWTLSRAISGNGITQHAAAGALAQVVGSGAFRGNDIGMVARVAAQMERSVGQSVSDT
                     INQFKRLKDDPVNAAKALDNELHFLTATQLEQIRVLGEQGRSSDAARIAMSALAEETG
                     RRTADIDNNLNALGSTLKYLSDLWSRFWDAAMNIGREDSLDEQIAALQEKVSRAKRLP
                     WTASSSQVEYDQQRLNDLQEKKRQKDLQDAKEQAERNYQEQQKRRNAENAALNRMNET
                     EAARHQREIARINAMQYADQAVRDAAIQRENERYEKALASGKKKTRETRNDEATRLLL
                     QYSQQQAQVEGQIAAARQSAGIATERMTEAHKQLLALQQRISDLDGKKLTADEKSVLA
                     RKDELIQALTLLDVKQQELQKQTALNELKKKTIQLTSQLAEEERAQRQQHDLDIATVG
                     MGDQQRQRYQVQLSLRQKYQQQLEQLRRDSEQKGTYNTDDYRKAEQALTESLNRQLNE
                     NRRYWQQLEVVQGNWKNGVLRAFQDFTVDADNTAETAEQVFSSAFSNMGNGLATFVTT
                     GKLNFKSFTSSVLSDMAKILAQATMMKSIKGIGSVLGFDLSSLSLNANGGIYQSADLS
                     RYSGTVVNRPTFFAFAKGAGVMGEAGPEAILPLRRGADGKLGVVADIGGSGMAMFSPQ
                     YNIEINNDGTNGQIGPAALKAVYDLGKKAAADFMQQQARDGGRLSGAYR"
     gene            12583..12912
                     /locus_tag="ACHOSC_RS11525"
                     /old_locus_tag="pgaptmp_002305"
     CDS             12583..12912
                     /locus_tag="ACHOSC_RS11525"
                     /old_locus_tag="pgaptmp_002305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HGU4548235.1"
                     /translation="METFHWKVRPDMNVVSEPKVVTVKLGDGYEQRRAAGLNNQLSTY
                     SVTIRVRKCDHPSLKAFLERHGGVRAFQWTPPYDWKPIRVVCRKWSASVGALWVTITA
                     DFEQVVA"
     gene            12922..13620
                     /locus_tag="ACHOSC_RS11530"
                     /old_locus_tag="pgaptmp_002306"
     CDS             12922..13620
                     /locus_tag="ACHOSC_RS11530"
                     /old_locus_tag="pgaptmp_002306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030430 - host cell cytoplasm [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0046718 - symbiont entry into host cell
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage minor tail protein L"
                     /protein_id="HGU4548236.1"
                     /translation="MQDIPQETHHETTRLTQSAQVVLWEIDLTEVGGERYFFCNEQNE
                     KGEPVIWQGRQYQVYPIQGTGFELNGKGSAARPTLTVSNLYGMVTGMAEDLQSLVGGT
                     VVRRKVYARFLDAVNFVNGNSDADPEQEVISRWRIEQCSELSAVSASFVLSTPTETDG
                     AVFPGRIMLANTCTWTYRGDECGYHGPAVADEYDQPTSDITKDKCSKCLSGCKFRNNV
                     GNFGGFLSINKLSQ"
     gene            13625..14368
                     /locus_tag="ACHOSC_RS11535"
                     /old_locus_tag="pgaptmp_002307"
     CDS             13625..14368
                     /locus_tag="ACHOSC_RS11535"
                     /old_locus_tag="pgaptmp_002307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001396591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C40 family peptidase"
                     /protein_id="HGU4548237.1"
                     /translation="MTETESAILAHARRCAPAESCGFVVRAPEGERYFPCVNISGEPE
                     DYFRMAPEDWLQAEMQGEIVALVHSHPGGLPWLSEADRRLQVQSDLPWWLVCRGAIHK
                     FRCVPHLTGRRFEHGVTDCYTLFRDAYHLAGIEMPDFHRGDDWWRNGQNLYLDNLEAT
                     GLYQVALSSAQPGDVLLCCFGSSVPNHAAIYCGDGELLHHIPEQLSKRERYTDKWQRR
                     THSLWRHRAWHASAFTGIYNDLAAASTFE"
     gene            14266..14913
                     /locus_tag="ACHOSC_RS11540"
                     /old_locus_tag="pgaptmp_002308"
     CDS             14266..14913
                     /locus_tag="ACHOSC_RS11540"
                     /old_locus_tag="pgaptmp_002308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_461526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tail assembly protein"
                     /protein_id="HGU4548238.1"
                     /translation="MAATHTLPLASPGMARICLYGDLQRFGRRIDLRVKTGSEAIRAL
                     AMQIPAFRQKLSDGWYQVRIAGRDAGETELSARLNEPLANGAVIHIVPRLAGAKSGGV
                     FQVVLGAALIAVAWWNPVGWLGAAAVSGMYAAGASMILGGVAQMLAPKARTPTAASTD
                     NGKQNTYFSSLDNMVAQGNVLPVLYGEMRVGSRVVSQEISTADEGDGGQVVVIGR"
     gene            14974..>16151
                     /locus_tag="ACHOSC_RS11545"
                     /old_locus_tag="pgaptmp_002309"
     CDS             14974..>16151
                     /locus_tag="ACHOSC_RS11545"
                     /old_locus_tag="pgaptmp_002309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HGU4548239.1"
                     /translation="MGKGSSKGHTPREAKDNLKSTQLLSVIDAISEGPIEGPVDGLKS
                     VLLNSTPVLDTEGNTNISGVTVVFRAGEQEQTPPEGFESSGSETVLGTEVKYDTPITR
                     TITSANIDRLRFTFGVQALVETTSKGDRNPSEVRLLVQIQRNGGWVTEKDITIKGKTT
                     SQYLASVVVDNLPPRPFSIRMRRMTPDSTTDQLQNKTLWSSYTEIIDVKQCYPNTALV
                     GVQVDSEQFGSQQVSRNYHLRGRILQVPSNYNPQTRQYSGIWDGTLKPAYSNNMAWCL
                     WDMLTHPRYGMGKRLGAADVDKWALYVIGQNCDQSVPDGFGGTEPRITCNAYLTTQRK
                     AWDVLSDFCSAMRCMPVWNGQTLTFVQNRPSDKAWTYNRSNVVMPDDGAPFRYSFSAL
                     "
BASE COUNT         3852 a         3805 c         4967 g         3527 t
ORIGIN      
        1 ccagatggag ttgcttatgt caaatgtgag cgggatcggt gatgcttatt actggagtgt
       61 ttttaaaatc gctgaggcct ttgggcttca ccgggacaca gtaaaaaaac ggctcctcgc
      121 ggctaacact cctgtggctg cgactgtcag ggggaacccc gtttacgccc tgcagcatgt
      181 cgggccagcc ctgtttagtg tgaagcatga ggcagcagac tctgttcatg atccatcccg
      241 tatggagccg aaagagagaa aggactggta ccaatctgaa aatgaaagga tcaagctgga
      301 aaaggagcag cgaaaactca tccccgttga tgaagtagtc atcgtctatt cgtccatgag
      361 aaaggctgtc gtccaggttc tggagacaat tccggatgtt cttgaacgcg attgcgccct
      421 gactcctcag gccgtcggcg ttgtacagca ggccattgat gacctgcgat acactcttca
      481 ggaaaaatcc tacgaggctt gtgctgctga attaattcct gatgaggaag gagagagtct
      541 ctaggaggaa taatgggttt ttcatcagcc cgaaatttgg gaagggacat atcggcagga
      601 ttttccccac cacgtcgcat gccgatttcg gaggctgtta aaaaattcat gcgtgttccc
      661 aagggggctg gtaactcggt gccatgggat cctgaactga caccctacat cattgagccc
      721 atgaactgcc tggcatcgcg tgaatacgat gcggtgattt ttgttggtcc tgcgcgaaca
      781 gggaagacca ttggtctgat cgatggatgg attgtctata ccatcgtttg cgatccttcg
      841 gacatgctcg ttgtgcagat gaccgaagat aaggcccgcg agcattctaa aaagcgcctc
      901 gacagaacgt tcagaagcag tgcggcggta aagaaaagaa tgagtccacg tcgtaacgac
      961 aataatgtcc atgataagac gttcagggat ggctcgttcc ttaaaattgg ttggccctcg
     1021 gtcaacatta tgtcgtcgtc ggattaccgg tttgtcgcct taaccgatta cgaccgtttt
     1081 ccggagaata tcgacagcga gggtgatggt ttctccctgg cctcaaaacg taccaccaca
     1141 tttatgtccg ctgggatgac tctggtggag agctcgccgg gacgtgacat ctgcgacagc
     1201 aaatggcgac gtaagtcgcc tcatgaagcg ccaccgacga ctggtattct ttccctttac
     1261 aatcgtggtg accgccgccg ctggtactgg tcatgtccgc actgcggtga atattttcag
     1321 ccagccatgg atgccatgac cggctaccgt aatgaaccgg atccctttaa agccagtgag
     1381 gcggcgtatc tactttgccc gcactgcagc ggcattatca ctgcggagaa aaagcgtgag
     1441 ctcaatagtg caggagtctg gttgcgtgaa ggtcaggtca ttgatcgtaa cggcaacgtt
     1501 tccggtgaac cgcgccgctc ccgtatcgcc agtttctgga tggaagggcc agctgctgcg
     1561 tatcagacct gggcgcaact ggtttacaaa ttactgactg cagaacagga gtatgaagcg
     1621 acaggaagcg aagaaacact cagggcggtt atcaacaccg actggggatt gccttatctt
     1681 cctcgtgcca gcatggagca acgaaaaagt gaactgcttg agcagcgggc agagccagtt
     1741 ccttcccgca gtgtgccgga tggcgttaat ttccttgtgg cgacagtgga tgtgcaggcg
     1801 ggacgtcatc gccgttttgt ggttcaggta acgggctatg gcagtcgtgg cgaacgctgg
     1861 attattgatc gttacaacat cacgcagtca ttgcgcagtg actgcgacgg ggagagccag
     1921 cgaattgatc cggccagcta tccggaagac tgggatgtcc tgctgacgga tgtttttcat
     1981 aaaagctggc cgctggcctc cgatccttct caacaaatgc gactgatggc aatggcggtg
     2041 gactccggcg gtgaagacgg ggtcactgat aatgcctata aattctggcg tcgttgccgt
     2101 cgtgatggcc ttggtaaacg tatttacctg tttaagggtg acagcatccg gcgcgcaaaa
     2161 ctgatcagcc gtacattccc tgataacacc ggacgaacgg gccgccgggc gcaggccgca
     2221 ggtgatgtgc cgctctggct tcttcagacg gatgccctga aagaccgggt gaataacgcg
     2281 ttatggcgtg actcgccagg tcctggctat gtgcatttcc ctgactggct ggggagctgg
     2341 ttttacgacg aactgacgta tgaagagcgg agcagtgacg ggaaatggag taagccgggt
     2401 cgcggtgcca acgaagcttt tgacctgatg gtgtatgccg aggctctggt cattctgcat
     2461 ggatacgaaa agatccgctg gccggatgca ccggagtggg cgagccggga gacctggctg
     2521 gagtgtgtcc aggacagtac cgaaccgtca tcctcaccgg aaccggtatc cacgcctgtt
     2581 aaaaaacaaa aacggaagaa aacagtaact gacgatgtta acccctggct gacttccgga
     2641 ggatggttat gaaccagaat gatatcgaag ccatgattca gcgttatacg gaagctgaaa
     2701 tggcggtgct ggacggaaaa tccgtcacct ttaatggtca gcagatgacc atggaaaact
     2761 tatctgagat ccggcaggga cggcaggagt gggagcgccg ccttgcggct ctgattacac
     2821 gacgacgggg gcatcccggg taccggctgg cgaggttctg atggcaattc ttgatgatgt
     2881 gattggcgtt ttttcaccag gatggaaagc ggcaaggctg cgttcccgtg cggtgatcca
     2941 ggcttatgag gccgtaaaaa cgacgcggac acacaaagcc cgacgggaga accgaactgc
     3001 cgaccagtta agccagtacg gggccgtgtc gttacgtgag caggcccgtt accttgataa
     3061 caaccacgat ctggttattg gtgtatttga caagctggaa gaacgggtgg tggggaaaaa
     3121 cgggattatt gtcgagccac atccggtatt acgcaatggg gccattgccc gtgacctggc
     3181 tgcggagatt cgcacccgat ggagtgaatg gtctgtcagc ccggaagtca ccgggcagtt
     3241 tacccgtccg atgctggaac gtctgatgct tcgtacctgg ctgcgcgatg gtgaggtgtt
     3301 tgcccagatg gtttccgggc gcataaacag cctggcgcct tctgccggtg ttcatttctg
     3361 gctggaggcg ctcgagccgg actttattcc catgactagt gatgagagca acaggctgaa
     3421 tcagggcgtg tttgttgatg actgggggcg tcccgaaaaa tatctggtgt ataaaagccg
     3481 tcccgtatcc ggacggcaga tggaaaccaa agaagtggat gcagagcgaa tgctgcatct
     3541 taaatttgtt cgccgtctgc accagatgcg cgggacgtct ttgttgtccg gtgtgctgat
     3601 ccgcctcagt gccctgaaag aatatgaaga ttctgagctg actgcagcaa ggatcgccgc
     3661 tgctctgggg atgtacatcc ggaaaggcga cgggcagagc tatgaaccgg atggtaatgg
     3721 cagaaaggag aatgaacgtg agcttaccat tcagccgggc attatttacg acgatctgaa
     3781 acccggcgaa gaaatcggaa tggtgaagtc ggatcgcccc aatcctaacc ttgaaacttt
     3841 tcgtaatggt cagttgcgtg ccgtggcggc gggcagtcgt ctgagttttt ccagtacagc
     3901 gcgcaactat aacggcactt acagcgccca gcgtcaggag ctggttgaat ccactgatgg
     3961 ctacctgatc ctgcaggact ggtttattgg tgccgtcacc cgcccgatgt atcgtgcatg
     4021 gctgaaacag gctgtggcat ccggtgttat caggctaccc cgcgatcttg accgttcttc
     4081 actgtatacc gcggtgtatt ccggaccagt gatgccgtgg attgaccctg ttaaggaggc
     4141 tgaggcctgg aaaatccaga ttcgtggtgg agcggcgaca gaatcagact gggtacgtgc
     4201 tggtggtcgt aatccggatg atgtcaaacg tcggcgcaag gccgaaattg atgaaaaccg
     4261 caagctggat ctggtatttg ataccgatcc ggccagtgat aaaggaggca gtagtgccgc
     4321 aacgaaacga caggagccgc agcacaccga cgaccagtcc gaagaataat tcctggttca
     4381 ggatgcaggc tggtcaccag agtgacgcgg atatttatat ttatgacgag attggtttct
     4441 ggggtgttac agcgaagcag tttatcagtg atctgaatgc actgggcgat atcacccaca
     4501 ttaatctcca tattaattca ccgggtggcg atgtctttga aggcatcgcc atttttaatg
     4561 cgctgaaaac acatggtgcg tccattaccg tttatgtcga cggtgtggcg gcgtcaatgg
     4621 cgtcggtcat tgcgatggta ggaaacccgg tcattatgcc ggaaaactcc ttcatgatga
     4681 ttcataaacc atttggcttt acgggcggtg atgcggagga catgcgcacc tatgccgacc
     4741 tgctcgataa agttgaggcg gttctgttac ccgcttatgc acagaaaacc gggaaaacca
     4801 ccgatgaaat tgctgccatg ctggcggatg agacctggat gtccggtgcc gaatgtctgg
     4861 cacagggatt tgctgatcag gtgacaccag ccgttaaggc aatggcatgt attcagtcaa
     4921 aacgtacaga ggaatttaaa aagatgccgg aatccattcg aaatatgatt actccgccac
     4981 gcaacagtgc tccacgcgta caggatgatg gacctgcagc ctcccggacg ccagtgcagg
     5041 cagcagcacc cgtggtggat gaaaacagca tccgtgcgca ggtcctggca gagcaaaaag
     5101 cgcgtgtaaa cggtattaat gatctgtttg ccatgtttgg cgggcgttat cagtcgttgc
     5161 aggctcagtg tcttgccgat cctgaatgtt cgctggagca ggcccgcgaa aagctgttga
     5221 acgagatggg gcgcgagtcc acgccatcca ataaaaatac cccggctcat atttatgccg
     5281 gtaacggtaa ttttgtgggg gacgggatcc gccaggcgct gatggcgcgc gccgggtttg
     5341 aaaaaactga acgtgataat gtctacaacg ggatgaccct gcgtgaatat gcccgtatgt
     5401 cactgactga acgtggtatt ggtgtttcca gttataaccc gatgcagatg gtcggtgcgg
     5461 cgttcacaca cagtacgtct gacttcggta atattctgct ggatgttgcg aacaaagcca
     5521 ttctgcaggg ctgggaagat gcccctgaaa cctatgaaca gtggacgcgg aaaggtcagt
     5581 tgtctgattt taaaattgcc catcgtgtgg gtatgggggg cttcagtgct ctgcgtcagg
     5641 tgcgtgaagg ggcagaatat aaatacgtca ccaccggaga taaacaggcc actattgcac
     5701 tggcgaccta tggcgagctg ttcagtatca cccgtcaggc cattatcaat gatgatctga
     5761 atatgctgac cgatgtcccg atgaagctgg gccgtgcggc gaaatccact attgccgatc
     5821 tggtttatgc cattctgacg tctaacccga aaatctccac agataatgta agtctgttcg
     5881 ataaagcgaa acatgcaaac gtactggaga gcgcggcaat ggacgtggca tcgctggata
     5941 aagcccgcca gttgatgcgc gttcagaaag agggtgagcg tcatctgaat attcgtcctg
     6001 cgttcgtact ggtaccgacg gcgatggagt ctgttgctaa ccaggtcatt cgctcctcaa
     6061 gtgtcaaggg ggctgacatt aacgccggta ttattaaccc ggtgaaagat tttgcgaccg
     6121 ttattgcaga gcctcgtctt gatgataaca gccagaccac cttctacctg gctgcgtcca
     6181 aaggctccga tacgattgaa gtggcttatc tcaacggtgt ggatacgcca tatattgatc
     6241 agatggaggg cttcagtgtg gatggcgtga caacgaaagt gcgtattgac gctggtgttg
     6301 cgccagttga tcaccgcggc ctggtgaaat gtacggcgta aacgtcgcag acaacaactc
     6361 tgatggcccg taagggcttt ttttgtacct gaaatcagcc cctgaacggg gctgtgcgga
     6421 gacagttatg gcaaagaatt ttgtagaaga aggaaaaacg gtggcgattg ttgccagtgc
     6481 agccatcagc agcggagatc tggtgcaggt gggggatgtt tttgcggtgg cgctgaccga
     6541 tattccacag ggagaaacag gcgacggcat aaccgaaggt gtgtttatgc tgcctaagct
     6601 gaaaacggat gacatgaaaa cgggtaagaa ggtttatctg aagtcaggaa aagttcagct
     6661 gactaacagc ggctctgagc cgctggtcgg ggttgtctgg gcagatgccg gaaccagtgc
     6721 agaagaagtg ccggtaaaac tcaatgtctg atcccttttc ccggctggca gcgcgtatgg
     6781 atgcgatcac ggtcagaaag atgggaaaga cagcctcgat taatgatgtc gatatgattg
     6841 tgatcccggg agaaacactg gcagagctga atgctctgtc cggacctgcg gtctctctgg
     6901 tggtgttttc ttcgggatac cgcccacggc gcggggatcg cgttgtttat gacggacaac
     6961 aatggacggt cacacggcat gaacgtttta acggtaagcc aatgatcttt attgagtaaa
     7021 gaggtgtggg atgaaggggc ttgagaatgc catccgcaat ctgaacagcc ttgatacccg
     7081 tatggtgcca caggccagcg catgggcgat aaaccgtgtg gcacagaaag cggtctcggt
     7141 tgccacccgg caggttgccg ggaataccgt tgcgggagat aaccaggtga aagggatccc
     7201 cctgaaactg gtacgtcagc gtgtccgggt gtttaaagcc agtccgtcag gaaaaatgac
     7261 ggtcaggatc cgcgttaacc ggggcaatct gcccgccatt aagctgggga cagcccgggt
     7321 cagactggcc cggcgtggtg gaaaactgca gtaccgtggc agtgtgctga aggtgggtaa
     7381 atatcttttc cgggatgcgt ttattcagca actggcgaat ggtcgctggc atgtgatgcg
     7441 acgtattgat ggcaaaaatc gttaccccat tgatgtggtg aaaatcccgc tgtccggatc
     7501 gctgacacag gcatttgaag atgcccgcga ccacatcatt gctgcggaaa tgccgaaaca
     7561 gctggggtat gcactaaaac aacaactgag gttatggctg acccgatgaa ccgacataca
     7621 caaatccgcc aggccgtact ggcacgcctt cgggaacagt gtggagacag cgccacgttt
     7681 tttgacgggc ttccggcatt tattgatgcg caggaactgc ctgccgtggc ggtgtggctg
     7741 agtgatgctc agtacaccgg aaaaatgacg gatgaagatg actggcaggc tgttctgcat
     7801 attgccgtct tcatccgggc acaggcaccg gattcagagc tggatatgtg gatggagagc
     7861 accattttcc ctgccctgaa tgatataccg gcactttccg gactcatcga caccctgatc
     7921 ccactcggtt ttaactatca acgtgataat gagatggcca cctgggcgat ggcggaaatc
     7981 acgtaccaga tcacgtacac gaattaagga ggtggtaatg accacaccaa atccactggc
     8041 aaagacgaaa ggtgcgggga cgacgttctg gatgtatacc ggcaacggcg atgcgtttgc
     8101 gaaccctttg tcggacactg actggctgcg tcttgcgatg gtgaaggatc tgcaacctgg
     8161 cgaaatgacc gctgatgcag aagatgacac ttatctcgat gatgaagatg cagactggaa
     8221 aacgacaacc caggggcaga aatccgttgg tgatacttcg gcgacgctgg cctggcgtcc
     8281 gggtgacagc gggcagaaaa aactggttca gttgttcgac tccggtgaag tctgcgcgtt
     8341 tcgtatcaaa tatcccaacg gcactgttga tgttttccgt ggctggctga gctcactggg
     8401 taaaaccatt gcctcaaaag acgtgatgac ccgcactgtg aaaatcagcg gtgtggggcg
     8461 tccgtatctg gcagaggaag gcactgaaac cgtgagcgtt accgggctga cagtatcacc
     8521 ggcgtctgcc agtgtaaaag tgggagcaac caccacgctg acctttacag taaaacctga
     8581 cggagccagt gacaaagcga tcagtgtgca ttcgtcagat ccacagactg ccacggttac
     8641 cctgaacgga cttgtggcca cggtaaaagg cgtgaagcag ggcagtgtca gcattgtggg
     8701 catgaccgct gacgggaatt ttgtggctgt gactgcggtg actgtcagcg cagcaggtta
     8761 acaggacgat actcatcatt tgccccggtt attcggggct tttttgcagg tggagaacat
     8821 gatgtttctg aaacaggaca cgtttaatta tgaaaaacag tccgtggtgc tcagtgagct
     8881 gtccgggctg cagagaattg aatatctgac gtttgttcag cagcgaacgg caaagtttga
     8941 tgccggggag ggagaactgc cggaggctga acgacagatt gcttttctgc ggatggggat
     9001 ggatatcaat gcctggctgg tttcccgctc actgtggaat gcggatcagt ctaaggatgt
     9061 agagacgctt tgcgcatccg ttattacaac atggtcgtat gatgccctgg gtgcgggggc
     9121 ggagatggtt ctgtcgctga gcggtatggg ggccattgat aatgccgtgg atgatgagca
     9181 tgaggcgctg acgccggaaa agtcctgacg cgggaaatgc agtttgtcat gcggcttgcc
     9241 cgggagttcc ggcgggcaga ctggcggcgg atgctgtcgg aaatgtcggc cactgagctt
     9301 ggtgagtggg gcgattattt ccggatgcag agcttcagtg atgtgtggat ggatgcgcag
     9361 tttgcctcgc tgaaggcatt gatcgtgaga atggtgtccg gcagcagtga tgctgcggtg
     9421 gctgatttca gccttttacc ggaagagaac gggataccgg agcgaacgga cgaagaactg
     9481 atgcatcttg gggaaggtat ttccggaggt gtgcgttatg gaccagatag ccaacctggt
     9541 cattgatttg gggattgatg cggcagagtt taaaaatgaa attccccgta tcaaaaacct
     9601 tctgaatggt gcagccagcg atgcagaacg gtcttctgcc cgtatgcagc gttttatgga
     9661 gcgtcagact caggccgccc ggcagacaac gcaggcagct tcttcggctg caacagccgc
     9721 atccgtccat gcgcagacgg tggagaagaa cgcacaggct catgaacgca tggcccgcga
     9781 ggtggagaaa acccgccagc gcatggaggc actgagccag aaaatgcgcg aggaacaggc
     9841 gcaggccatg gctctggcgg aggctcagga taaagcggct gctgcgtttt atcgtcagat
     9901 tgacagtgtg aaacaggcca gtgcggggct gcaggaatta cagcgtattc agcagcagat
     9961 ccgacaggcc agaaacagtg gcgggattgg tcagcaggat tatctggcgc tgatttctga
    10021 ggttactgcg aaaacccgtg ttcttacaca ggctgaggaa gaggctaccc gacagaaagt
    10081 ggcgtttatc cgtcagctta aagagcaggc aacccgcctg aatctttcat cttctgagtt
    10141 gcttcgtgct aaggcagccc agctgggggt aagcagtgct gcagaagtgt atatccgcaa
    10201 aatggagcag gcaggaaaag ccacgcattc gctgggtctg aaaagtgcag cagcccgcca
    10261 ggagataggc gttctgatag gtgaactggc ccgcggcaat ttaggggcgc tgaggggatc
    10321 cgggataacg ctggctaacc gtgccggatg gatagacaca ctgatgtcac cgaaaggcat
    10381 gatgcttggc ggggttattg gcggtattgc cgcggccgtc tatggtctgg gtaaagcctg
    10441 gtatgacggt cagaaggagg gggaagaatt taaccgccag ttgtcgctga cggggcatta
    10501 tgccggagtc actgccgggc agctgtggac gctcagtcgt gctatttccg ggaatggtat
    10561 cacgcaacat gctgcagccg gtgcgctggc tcaggtggtg gggagtggtg catttcgtgg
    10621 aaacgatatc ggtatggtgg cgagagttgc cgcacagatg gagcgatcgg ttggccagtc
    10681 ggtcagcgat accataaatc agtttaagcg gctgaaggat gatcctgtaa atgccgcgaa
    10741 ggctctggac aatgagctgc attttcttac tgccactcag cttgagcaga tacgcgtcct
    10801 tggggagcag gggcggtcca gtgatgctgc acggatagcc atgtctgcac tggcagagga
    10861 aaccggtcgg cgtactgcgg atattgataa taacctcaat gcgcttggca gtacgctgaa
    10921 gtatctgtct gatttgtgga gtcgtttctg ggatgcggcc atgaatattg gtcgtgaaga
    10981 ctcgctggat gaacagattg ccgctttaca ggagaaagtg tcgcgggcga aaagactccc
    11041 ctggacggca tcatcttctc aggttgaata cgatcagcag cgtcttaacg atcttcagga
    11101 gaaaaaacgc cagaaggatt tgcaggatgc aaaagagcag gcagagcgga attatcagga
    11161 gcaacagaaa cgccgtaatg ctgaaaatgc tgcactgaac cggatgaatg aaacggaagc
    11221 agcacgacat cagcgtgaaa ttgcgcgtat taatgccatg cagtacgccg atcaggctgt
    11281 cagggatgcg gcgatacaac gtgaaaatga acgttacgag aaagccctgg catccggtaa
    11341 gaaaaaaaca cgcgaaaccc gtaatgatga ggccacccgg ttattgctgc agtacagtca
    11401 gcaacaggca caggtggaag gacagattgc tgctgccaga cagtcagcag gcattgccac
    11461 ggaaaggatg acagaagcgc ataaacagct tctggctctg cagcagcgca tcagcgacct
    11521 ggacgggaaa aaactgacgg cagatgaaaa gagtgtgctg gcccgtaaag atgaactgat
    11581 tcaggcactg acgctgctgg atgtaaaaca gcaggagctt cagaaacaga cggcactcaa
    11641 cgagctgaag aaaaaaacaa ttcagctgac cagtcaactg gctgaagaag agcgcgctca
    11701 gcgtcagcaa catgacctgg atatcgccac ggtgggtatg ggtgatcagc agcgacagcg
    11761 atatcaggta caactgagtc ttcgccagaa ataccagcaa cagctggagc agttgaggcg
    11821 ggatagtgag cagaaaggga catataacac ggatgactac agaaaggccg agcaggcgct
    11881 gacggagagc ctgaaccgac aactgaatga gaatcgccgt tactggcaac agcttgaagt
    11941 tgtgcagggt aactggaaaa acggagtcct gcgtgcattt caggatttta ccgtggatgc
    12001 agataatacg gcagaaacag cagaacaggt gttctcgtca gccttcagca acatgggaaa
    12061 tggcctggca acttttgtca ctaccggcaa actcaatttc aaatccttca cctcttctgt
    12121 gctgtcagat atggcgaaaa tcctggcgca ggcaaccatg atgaaatcga taaaagggat
    12181 tggcagtgta ctgggatttg atctcagcag cctttccctg aatgccaatg gggggattta
    12241 tcagtctgct gatttgagtc gttacagtgg cacggtggtt aaccgtccga cgttttttgc
    12301 ttttgcaaaa ggcgcgggtg tgatggggga agcgggacct gaagccattc tgccattgcg
    12361 tcgtggtgct gacggtaagc tgggggttgt ggcggatatt ggggggtcag gtatggcgat
    12421 gttttccccg cagtacaaca tcgagatcaa taacgatggc acgaacgggc agataggtcc
    12481 ggctgccctg aaggcggttt atgacctcgg gaaaaaagcg gcagcggact ttatgcaaca
    12541 gcaggcccgt gatggtggtc ggttaagtgg agcatatcgg taatggagac gtttcactgg
    12601 aaagtgcgcc cggatatgaa tgtggtatca gagccgaaag tggtgacagt gaagctgggc
    12661 gatggttatg aacagcgtcg tgcggcggga ctgaataacc agttgtcgac ttacagcgtg
    12721 acgatacgtg ttcgtaaatg tgaccaccca tctttaaaag cctttctgga acggcacggt
    12781 ggcgtccgtg catttcagtg gacgccacct tatgactgga aaccgatcag ggtggtttgt
    12841 cgtaaatggt cggcaagcgt gggggcgctg tgggtaacca taacggcaga ttttgaacag
    12901 gtcgtggcat aggaggctct gatgcaggat attccacagg aaacacatca tgagacgaca
    12961 cgcctcactc agtcagccca ggtggtgctc tgggaaatcg atctgacaga ggtcggtggt
    13021 gaacgttatt ttttctgtaa tgagcagaac gaaaaaggtg agccggttat ctggcagggg
    13081 cggcagtatc aggtataccc cattcagggg acgggatttg aactgaacgg caagggcagt
    13141 gctgcccgtc cgacactgac ggtttctaac ctgtacggta tggtcaccgg gatggcggaa
    13201 gatctgcaga gtctggtcgg cggaacggtg gtccggcgta aggtttacgc ccgttttctg
    13261 gatgcggtga acttcgtcaa cggaaacagt gacgccgatc cggagcagga ggtgatcagc
    13321 cgctggcgca ttgagcagtg cagcgaactg agcgcggtga gtgcctcttt tgtactgtcc
    13381 acgccgacgg aaacggacgg cgctgttttt ccgggacgta tcatgctggc caacacctgc
    13441 acctggacct atcgcggtga tgagtgcggt tatcacggtc cggcagtcgc ggatgaatat
    13501 gaccagccga cgtccgatat cacgaaggat aaatgcagca aatgcctgag cggctgtaag
    13561 tttcgcaata acgtcggcaa ctttggcggc ttcctttcca ttaacaaact ttcgcagtaa
    13621 tcccatgaca gagacagaat cagcgattct ggcgcacgcc cggcgatgtg cgccagcgga
    13681 gtcgtgcggc ttcgtggtga gagcgccgga gggggaaaga tattttccct gcgtgaatat
    13741 ttccggtgag ccggaggatt atttccggat ggctccggag gactggctgc aggcagagat
    13801 gcagggtgag attgtggcac tggtccacag tcatcccggt ggtctgccct ggctgagtga
    13861 ggctgaccgg cggctgcagg tgcagagtga tttgccgtgg tggctggtct gccggggggc
    13921 gattcacaag ttccgctgtg tgccgcatct caccgggcgg cgctttgagc acggggtgac
    13981 ggactgttac acgctgttcc gggatgctta tcatctggcg gggattgaga tgccggattt
    14041 tcatcgcggg gatgactggt ggcgtaacgg tcagaatctc tatctggata atctggaggc
    14101 cacagggctg tatcaggtgg cgttgtcatc agcacaaccg ggcgatgtgc tgctgtgctg
    14161 ttttggttca tcggtgccga atcatgccgc catttactgc ggcgacggtg agctgctgca
    14221 ccatattcct gaacaactga gtaaacgaga gaggtatacc gacaaatggc agcgacgcac
    14281 acactccctc tggcgtcacc gggcatggca cgcatctgcc tttacgggga tttacaacga
    14341 tttggccgcc gcatcgacct tcgagtgaaa acggggtctg aagccatccg ggcgctggcc
    14401 atgcagatcc cggcgtttcg tcagaaactg agcgacggct ggtaccaggt acgcattgcc
    14461 gggcgtgatg caggtgaaac cgaattgtct gcccgtctta atgagccgct ggcaaatggt
    14521 gccgtgatcc acatcgtgcc gcgtctggcg ggagctaaaa gtggcggtgt gtttcaggtg
    14581 gtgctggggg cggcgctgat tgcggtggca tggtggaacc ctgtgggctg gctgggtgcc
    14641 gcggctgtat cgggcatgta tgcggcaggg gccagtatga tcctgggcgg agtggcgcag
    14701 atgctggcac cgaaagccag gacgcccacg gcagcaagta cagataacgg caaacagaac
    14761 acctatttct cctcactgga taacatggtt gcccagggca atgttctgcc cgttctgtac
    14821 ggtgaaatgc gcgtggggtc gcgggtggtc tctcaggaga tcagcacggc agacgaaggg
    14881 gatggtggtc aggttgtagt gattggtcgc tgatgaaaaa cgttttatgt gaaaccgcct
    14941 ccgggcggtt ttgtcgttta tggagcgtta ggaatgggta aaggcagcag taaggggcat
    15001 actccgcgcg aagcgaagga caacctgaag tccacgcagt tgctgagtgt gatcgatgcc
    15061 atcagcgaag ggccgattga aggtccggtg gatggattaa aaagcgtgct gctgaacagt
    15121 acgccagtgc tggacactga ggggaatacc aacatctccg gcgtcacggt ggtgttccgg
    15181 gccggtgagc aggagcagac accgccggag ggatttgaat cctccggatc cgagacggtg
    15241 ctggggacgg aagtgaaata cgacacgccg atcacccgga ccatcacgtc ggcaaacatc
    15301 gaccgtctgc gctttacctt cggtgtgcag gcactggtgg aaaccacctc aaagggggac
    15361 aggaatccat cggaagtccg cctgctggtt cagatacaac gtaacggtgg ctgggtgacg
    15421 gaaaaagaca tcaccattaa aggcaaaacc acctcgcagt acttggcctc tgtggtggtg
    15481 gataacctgc cgccgcgccc gttcagtatc cggatgcgca ggatgacgcc ggacagcacc
    15541 acagaccagc tgcagaacaa aacgctctgg tcgtcataca ccgaaatcat cgatgtgaaa
    15601 cagtgctacc cgaacacggc actggtcggc gtacaggtgg attcggagca gttcggcagc
    15661 cagcaggtga gccgtaatta tcatctgcgc gggcgtattc tgcaggtgcc gtcgaattat
    15721 aacccgcaga cgcgacaata cagcggtatc tgggacggaa cgttaaaacc ggcatacagc
    15781 aacaacatgg cctggtgtct gtgggatatg cttacccatc cgcgctacgg catggggaaa
    15841 cgtcttggtg cggcggatgt ggataaatgg gcgctgtatg tcatcggcca gaattgcgac
    15901 cagtcagtgc cggacggctt tggcggcacg gagccgcgca tcacctgtaa tgcgtacctg
    15961 accacacagc gcaaggcgtg ggacgttctc agtgatttct gctcggcgat gcgctgtatg
    16021 ccggtatgga acgggcagac gctgacgttc gtgcagaacc gaccgtcgga taaggcgtgg
    16081 acctataacc gcagtaatgt ggtgatgccg gatgatggcg cgccgttccg ctacagcttc
    16141 agcgccctga a
//
LOCUS       DAZKHF020000086        16146 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2201S31241
            SAMN43267800-rid25106413.denovo.087, whole genome shotgun sequence.
ACCESSION   DAZKHF020000086 DAZKHF020000000
VERSION     DAZKHF020000086.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267800
            Sequence Read Archive: SRR30307263
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 16146)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16146)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 16146)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350581.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350581.2
            Genome Coverage       :: 151x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:21:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,652
            CDSs (total)                      :: 5,557
            Genes (coding)                    :: 5,320
            CDSs (with protein)               :: 5,320
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 237
            CDSs (without protein)            :: 237
            Pseudo Genes (ambiguous residues) :: 0 of 237
            Pseudo Genes (frameshifted)       :: 102 of 237
            Pseudo Genes (incomplete)         :: 130 of 237
            Pseudo Genes (internal stop)      :: 52 of 237
            Pseudo Genes (multiple problems)  :: 41 of 237
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16146
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267800-rid25106413.denovo.087"
                     /serotype="O 26:H11"
                     /isolate="2201S31241"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-01"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            61..1116
                     /gene="phoE"
                     /locus_tag="ACHOSC_RS11550"
                     /old_locus_tag="pgaptmp_002310"
     CDS             61..1116
                     /gene="phoE"
                     /locus_tag="ACHOSC_RS11550"
                     /old_locus_tag="pgaptmp_002310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_process: GO:0034220 - monoatomic ion transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoporin PhoE"
                     /protein_id="HGU4548240.1"
                     /translation="MKKSTLALVVMGIVASASVQAAEIYNKDGNKLDVYGKVKAMHYM
                     SDNASKDGDQSYIRFGFKGETQINDQLTGYGRWEAEFAGNKAESDTAQQKTRLAFAGL
                     KYKDLGSFDYGRNLGALYDVEAWTDMFPEFGGDSSAQTDNFMTKRASGLATYRNTDFF
                     GVIDGLNLTLQYQGKNENRDVKKQNGDGFGTSLTYDFGGSDFAISGAYTNSDRTNEQN
                     LQSRGTGKRAEAWATGLKYDANNFYLATFYSETRKMTPITGGFANKTQNFEAVAQYQF
                     DFGLRPSLGYVLSKGKDIEGIGDEDLVNYIDVGATYYFNKNMSAFVDYKINQLDSDNK
                     LNINNDDIVAVGMTYQF"
     gene            complement(1155..1556)
                     /gene="crl"
                     /locus_tag="ACHOSC_RS11555"
                     /old_locus_tag="pgaptmp_002311"
     CDS             complement(1155..1556)
                     /gene="crl"
                     /locus_tag="ACHOSC_RS11555"
                     /old_locus_tag="pgaptmp_002311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0045893 - positive regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma factor-binding protein Crl"
                     /protein_id="HGU4548241.1"
                     /translation="MTLPSGHPKSRLIKKFTALGPYIREGKCEDNRFFFDCLAVCVNV
                     KPAPEVREFWGWWMELEAQESRFTYSYQFGLFDKAGDWKSVPVKDTEVVERLEHTLRE
                     FHEKLRELLTTLNLKLEPADDFRDEPVKLTA"
     gene            complement(1614..2858)
                     /gene="frsA"
                     /locus_tag="ACHOSC_RS11560"
                     /old_locus_tag="pgaptmp_002312"
     CDS             complement(1614..2858)
                     /gene="frsA"
                     /locus_tag="ACHOSC_RS11560"
                     /old_locus_tag="pgaptmp_002312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="esterase FrsA"
                     /protein_id="HGU4548242.1"
                     /translation="MTQANLSETLFKPRFKHPETSTLVRRFNHGAQPPVQSALDGKTI
                     PHWYRMINRLMWIWRGIDPREILDVQARIVMSDAERTDDDLYDTVIGYRGGNWIYEWA
                     TQAMVWQQKACAEEDPQLSGRHWLHAATLYNIAAYPHLKGDDLAEQAQALSNRAYEEA
                     AQRLPGTMRQMEFTVPGGAPITGFLHMPKGDGPFPTVLMCGGLDAMQTDYYSLYERYF
                     APRGIAMLTIDMPSVGFSSKWKLTQDSSLLHQHVLKALPNVPWVDHTRVAAFGFRFGA
                     NVAVRLAYLESPRLKAVACLGPVVHTLLSDFKCQQQVPEMYLDVLASRLGMHDASDDA
                     LRVELNRYSLKVQGLLGRRCPTPMLSGYWKNDPFSPEEDSRLITSSSADGKLLEIPFN
                     PVYRNFDKGLQEITGWIEKRLC"
     gene            complement(2950..3408)
                     /gene="gpt"
                     /locus_tag="ACHOSC_RS11565"
                     /old_locus_tag="pgaptmp_002313"
     CDS             complement(2950..3408)
                     /gene="gpt"
                     /locus_tag="ACHOSC_RS11565"
                     /old_locus_tag="pgaptmp_002313"
                     /EC_number="2.4.2.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000310 - xanthine
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine phosphoribosyltransferase"
                     /protein_id="HGU4548243.1"
                     /translation="MSEKYIVTWDMLQIHARKLASRLMPSEQWKGIIAVSRGGLVPGA
                     LLARELGIRHVDTVCISSYDHDNQRELKVLKRAEGDGEGFIVIDDLVDTGGTAVAIRE
                     MYPKAHFVTIFAKPAGRPLVDDYVVDIPQDTWIEQPWDMGVVFVPPISGR"
     gene            3669..5126
                     /gene="pepD"
                     /locus_tag="ACHOSC_RS11570"
                     /old_locus_tag="pgaptmp_002314"
     CDS             3669..5126
                     /gene="pepD"
                     /locus_tag="ACHOSC_RS11570"
                     /old_locus_tag="pgaptmp_002314"
                     /EC_number="3.4.13.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytosol nonspecific dipeptidase"
                     /protein_id="HGU4548244.1"
                     /translation="MSELSQLSPQPLWDIFAKICSIPHPSYHEEQLAEYIVGWAKEKG
                     FHVERDQVGNILIRKPATAGMENRKPVVLQAHLDMVPQKNNDTVHDFTKDPIQPYIDG
                     EWVKARGTTLGADNGIGMASALAVLADENVVHGPLEVLLTMTEEAGMDGAFGLQSNWL
                     QADILINTDSEEEGEIYMGCAGGIDFTSNLHLDREAVPAGFETFKLTLKGLKGGHSGG
                     EIHVGLGNANKLLVRFLAGHAEELDLRLIDFNGGTLRNAIPREAFATIAVAADKVDVL
                     KSLVNTYQEILKNELAEKEKNLALLLDSVANDKAALIAKSRDTFIRLLNATPNGVIRN
                     SDVAKGVVETSLNVGVVTMTDNNVEIHCLIRSLIDSGKDYVVSMLDSLGKLAGAKTEA
                     KGAYPGWQPDANSPVMHLVRETYQRLFNKTPNIQIIHAGLECGLFKKPYPEMDMVSIG
                     PTITGPHSPDEQVHIESVGHYWTLLTELLKEIPAK"
     gene            complement(5183..5797)
                     /gene="prfH"
                     /locus_tag="ACHOSC_RS11575"
                     /old_locus_tag="pgaptmp_002315"
     CDS             complement(5183..5797)
                     /gene="prfH"
                     /locus_tag="ACHOSC_RS11575"
                     /old_locus_tag="pgaptmp_002315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000602105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003747 - translation release factor
                     activity [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor H"
                     /protein_id="HGU4548245.1"
                     /translation="MILLQLSSAQGPEECCLAVKKALDRLIKEAARQDVAVTVLETET
                     GHYSDTLRSALISLDGDNAWALSESWCGTIQWICPSPYRPHHGRKNWFLGIGRFTADE
                     QEQSDAIRYETLRSSGPGGQHVNKTDSAVRATHLASGISVKVQSERSQHANKRLARLL
                     IAWKLEQQQQENSAVLKSLRRMFHHQIERGNPRRTFTGMAFIEG"
     gene            complement(5794..6933)
                     /locus_tag="ACHOSC_RS11580"
                     /old_locus_tag="pgaptmp_002316"
     CDS             complement(5794..6933)
                     /locus_tag="ACHOSC_RS11580"
                     /old_locus_tag="pgaptmp_002316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000521550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008452 - RNA ligase activity [Evidence
                     IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA ligase RtcB family protein"
                     /protein_id="HGU4548246.1"
                     /translation="MGNYIRPLSDAVFTIASDDLWIESLAIQQLHTTANLPNMQRVVG
                     MPDLHPGRGYPIGAAFFSVGRFYPALVGNDIGCGMALWQTDILARKYNADKFEKRLSD
                     LDDVAEESWLEENLPSAFAQHPWRSSLGSIGGGNHFAELQQVDQIINAELFALAGLDA
                     QHLQLLVHSGSRGVGQSILQRHIASFSHHGLPQGSDDALRYIAEHDDALAFACINRQM
                     IALRIMQQVKATGSPVLDVAHNFVSACQIGDQQGWLHRKGATPDDNGLVIIPGSRGDY
                     SWIVQPVANEKTLHSLAHGAGRKWGRTECKGRLAAKYTATQLSRTELGSRVICRDKQL
                     IFEEAPQAYKSAESVVQCLVQAGLIIPVARLRPVLTLKNSGGKKG"
     gene            complement(7179..7631)
                     /locus_tag="ACHOSC_RS11585"
                     /old_locus_tag="pgaptmp_002317"
     CDS             complement(7179..7631)
                     /locus_tag="ACHOSC_RS11585"
                     /old_locus_tag="pgaptmp_002317"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HGU4548247.1"
                     /translation="MNNIQIRDYQPGDFQQLCAIFIRAVTMTASQHYSPQQIAAWAQI
                     DESRWKEKLAKSQVRVAVINAKLVGFITCVEHYIDMLFVDPEYTRRGVASALLKPLIK
                     SESELTVDASITAKPFFERYGFQTVKQQRVECRGEWFINFYMRYKQQH"
     gene            complement(7628..8683)
                     /gene="dinB"
                     /locus_tag="ACHOSC_RS11590"
                     /old_locus_tag="pgaptmp_002318"
     CDS             complement(7628..8683)
                     /gene="dinB"
                     /locus_tag="ACHOSC_RS11590"
                     /old_locus_tag="pgaptmp_002318"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003684 - damaged DNA binding [Evidence
                     IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase IV"
                     /protein_id="HGU4548248.1"
                     /translation="MRKIIHVDMDCFFAAVEMRDNPALRDIPIAIGGSRERRGVISTA
                     NYPARKFGVRSAMPTGMALKLCPHLTLLPGRFDAYKEASNHIREIFSRYTSRIEPLSL
                     DEAYLDVTDSVHCHGSATLIAQEIRQTIFSELQLTASAGVAPVKFLAKIASDMNKPNG
                     QFVITPAEVPAFLQTLPLAKIPGVGKVSAAKLEAMGLRTCGDVQKCDLVMLLKRFGKF
                     GRILWERSQGIDERDVNSERLRKSVGVERTMAEDIHHWSECEAIIERLYPELERRLAK
                     VKPDLLIARQGVKLKFDDFQQTTQEHVWPRLNKADLIATARKTWDERRGGRGVRLVGL
                     HVTLLDPQMERQLVLGL"
     gene            complement(8754..9677)
                     /gene="lafU"
                     /locus_tag="ACHOSC_RS11595"
                     /old_locus_tag="pgaptmp_002319"
     CDS             complement(8754..9677)
                     /gene="lafU"
                     /locus_tag="ACHOSC_RS11595"
                     /old_locus_tag="pgaptmp_002319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001521902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative lateral flagellar export/assembly
                     protein LafU"
                     /protein_id="HGU4548249.1"
                     /translation="MRKTGNRRERGAKTTIVRRQIKKNHAGHHGGAWKVAFADFTLAM
                     MALFMTLWIVNSVSKSERESIIAALHGQSIFNGGGLSPLNKISPSHPPKPATVAVPEE
                     TEKKARDVNEKTALLKKKSATELGELATSINTIARDAHMEANLEMEIVPQGLRVLIKD
                     DQNRNMFERGSAQIMPFFKTLLVELAPVFDSLDNKIIITGHTDAMAYKNNIYNNWNLS
                     GDRALSARRVLEEAGMPEDKVMQVSAMADQMLLDSKNPQSAGNRRIEIMVLTKSASDT
                     LYQYFGQHGDKVVQPQVQKLDKQQVLSQRTR"
     gene            complement(9680..10543)
                     /gene="motA"
                     /locus_tag="ACHOSC_RS11600"
                     /old_locus_tag="pgaptmp_002320"
     CDS             complement(9680..10543)
                     /gene="motA"
                     /locus_tag="ACHOSC_RS11600"
                     /old_locus_tag="pgaptmp_002320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001169515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor stator protein MotA"
                     /protein_id="HGU4548250.1"
                     /translation="MQKILGLLVVLGCVFGGYFEAGGQLVAIWQPAEMIIILGAGFGA
                     MIIGNPKHVLKEIAHQIKGVISKKQLGPEFQRQLLMCLYELLEMVQNGGLRMLDQHIE
                     QPEESTIFQKYPLVLTQKRLVTFIADNFRLMAMGKIDAHELEGILDQELDTAEESLLT
                     PSRSLQRTAEAMPGFGICAAVLGIIITMQSIDGSIALIGLKVAAALVGTFLGVFICYC
                     LMDPLANAMEQQARAEHSLLECVRTVLVAQAGGKPTLLAVDAGRKLLHLASKPTFANL
                     DAWVNAMLEQE"
     gene            complement(10556..11272)
                     /locus_tag="ACHOSC_RS11605"
                     /old_locus_tag="pgaptmp_002321"
     CDS             complement(10556..11272)
                     /locus_tag="ACHOSC_RS11605"
                     /old_locus_tag="pgaptmp_002321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000938732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FliA/WhiG family RNA polymerase sigma factor"
                     /protein_id="HGU4548251.1"
                     /translation="MLQDVFESEEFAAAPVLTPQQESHYLQAYLPLVRKVVRQLAPQC
                     NCIIDRQDMEQTALMGLLNAIRRYGLPDEGFAGYAVHRIRGAILDELRALDWRPRQLR
                     QKYHQMKDLIRETRKKLGHEPEWSELAVEGISHEDYLEYQQLEGAETLASLDELLNGE
                     GPGIPLVGRELEDQFVTQQMLQQALSQLSEKEQLILSMYYQHEMNLKEIALVLGLTEA
                     RICQLNKQIAQKIRDFVYPN"
     gene            complement(11292..11759)
                     /locus_tag="ACHOSC_RS11610"
                     /old_locus_tag="pgaptmp_002322"
     CDS             complement(11292..11759)
                     /locus_tag="ACHOSC_RS11610"
                     /old_locus_tag="pgaptmp_002322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001521900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009425 - bacterial-type flagellum basal
                     body [Evidence IEA];
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body-associated FliL family
                     protein"
                     /protein_id="HGU4548252.1"
                     /translation="MKKIVVAGVVSAVLALVVGAGAGWGVWHHYAGKGKPTAAAQTET
                     VETLDESKSVFVTLPETIVTLHDNNGADHYLSAELVMVVASDKEAEKIKHQEPLYQSI
                     AVECLTEMKFEDLRGMKISAIRKLISDALKKDLQRRKMSAPYKDLLVKKVVFQ"
     gene            complement(11767..12828)
                     /locus_tag="ACHOSC_RS11615"
                     /old_locus_tag="pgaptmp_002323"
     CDS             complement(11767..12828)
                     /locus_tag="ACHOSC_RS11615"
                     /old_locus_tag="pgaptmp_002323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001067462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-length control protein FliK"
                     /protein_id="HGU4548253.1"
                     /translation="MNPTLLATLGTLAETASLKADILPPVSGENAPAFTLPKMAVAAV
                     AERVHSAKTSQQQATRPQENDPVAMQALMALLLPQPAAPHQDTPQPRNVATSPVIQQL
                     TKAVVQNAPQRQTQQQELTPLPPQLQELISQLPQAKPEQQARLATYASEDLHAITPTQ
                     PRVSTQPARPKSELTRVTARPQVERKTEKVPDSEPVIARAVLQVKTPELLSDHQEIVA
                     KPATLSMDELGEKLTTLLKDQIHFQLNKQQQISTIRLDPPSLGKLEIAVQLDNGKLMV
                     HIGANQSEVCRALQQFSDDLRQHLTAQNFMEVNVQVSSEGQSQQQQQSGHQQEEVSAA
                     LQLDDAPQFQQNESVLIKV"
     gene            complement(12825..13136)
                     /locus_tag="ACHOSC_RS11620"
                     /old_locus_tag="pgaptmp_002324"
     CDS             complement(12825..13136)
                     /locus_tag="ACHOSC_RS11620"
                     /old_locus_tag="pgaptmp_002324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000414995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar protein FliT"
                     /protein_id="HGU4548254.1"
                     /translation="MEKQRRKLFALVEAMNEALDKQRWRRLPALHQQLIRQFHDYAAA
                     ETDAAQLDAVKARLYDAFNALIKRREQRAEVLKARMEQHQRHQEGVLAYSIVNLLSEK
                     S"
     gene            complement(13141..13533)
                     /gene="fliS"
                     /locus_tag="ACHOSC_RS11625"
                     /old_locus_tag="pgaptmp_002325"
     CDS             complement(13141..13533)
                     /gene="fliS"
                     /locus_tag="ACHOSC_RS11625"
                     /old_locus_tag="pgaptmp_002325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000285320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0044780 - bacterial-type flagellum assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar export chaperone FliS"
                     /protein_id="HGU4548255.1"
                     /translation="MYQVQDGYSQYKEIDLAARTAAASPLELVLVLFSGLMDELERAK
                     SHIEGRRFEKKAQSINKCIDILNALTSSLEFETGGELVVNLSRLYDHCVYRLYEASGE
                     LSAEKIDEVMLILSNLREGWEGLSGKLG"
     gene            complement(13556..14872)
                     /gene="fliD"
                     /locus_tag="ACHOSC_RS11630"
                     /old_locus_tag="pgaptmp_002326"
     CDS             complement(13556..14872)
                     /gene="fliD"
                     /locus_tag="ACHOSC_RS11630"
                     /old_locus_tag="pgaptmp_002326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000609688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009288 - bacterial-type flagellum
                     [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar filament capping protein FliD"
                     /protein_id="HGU4548256.1"
                     /translation="MINPRTIAQEIAYADVATQAANLQEKQTELDAESSGLDSLSSAL
                     SDFQSAVDALNSDTDGPVTFAATSNNDSASVSANSQAQAGSYSFFVEQLAQGQQTTFS
                     MGDDAFSATGTFELTMGDSTMDIDLSAADQNGDGDGFIDASELVNAINDSDDNPGVSA
                     ALVKTDGTTTIMLTSDSTGAQSAFSVSVTGHDASNDSTSAPVATDVSSAQDAIIHLGS
                     ATGPEITNSSNTFDDVIPGVTMTFTEVSDSDSDLTTFNISEDSSASQEKVQTFVDAYN
                     TLIDTVDSLTTHGDDSTSAGVFAGDAGLSSLANQLDDIAHASYNGVSIVDYGITLDSH
                     GHLQIDSDQFNDEMAKNPDGLTSIFVGDNSMVAQMDDLINTYTDSSNGIITLRQQNID
                     DQMSKIQDEGDQLTDTYNANYDRYLEEYTNTLVEVYTMKASMAAFA"
     gene            complement(15079..15993)
                     /gene="lafA"
                     /locus_tag="ACHOSC_RS11635"
                     /old_locus_tag="pgaptmp_002327"
     CDS             complement(15079..15993)
                     /gene="lafA"
                     /locus_tag="ACHOSC_RS11635"
                     /old_locus_tag="pgaptmp_002327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001570969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lateral flagellin LafA"
                     /protein_id="HGU4548257.1"
                     /translation="MLSINTNNASMAAVNAISKSSSSLSTSMERLATGNRINSSADDA
                     AGKQIANRLTAQSSGMGVALSNINDATAMLQTADSMFDEMSDVLGRMKDLSTQAANGT
                     YSDDDLQAMQDEYDELGQQMSDMLQNTTYGGTNLFGVSGTSNTGTDGLFQSAVTFQVG
                     AESSDTMTVNISSQLNTLVTDLSAISNSFSADQADTTGTAGVSGGTELTASGSANQMI
                     TSISTAMDDVSQIQSKLGASINRLNDTANNLTSMQDNTEVAIGNIMDTDYATEASNMT
                     KQQVLMQTGITMLKQSNSMSSMVSSLLQ"
BASE COUNT         3761 a         4307 c         4035 g         4043 t
ORIGIN      
        1 ctgttttcaa cggggctctc ccgctgaata ttcgcgcgtt aattaaaatc aggaatgaaa
       61 atgaaaaaga gcactctggc attagtggtg atgggcattg tggcatctgc atctgtacag
      121 gctgcagaaa tatataataa agacggtaat aaactggatg tctatggcaa agttaaagcc
      181 atgcattata tgagtgataa cgccagtaaa gatggcgacc agagttatat ccgttttggt
      241 tttaaaggtg aaacacaaat taacgatcaa ctgactggtt atggtcggtg ggaagcggag
      301 tttgccggaa ataaagcgga gagtgatacc gcacagcaaa aaacgcgtct cgcttttgcc
      361 gggttgaaat ataaagattt gggttctttc gattatggtc gtaacctggg cgcgttgtat
      421 gacgtggaag cctggaccga tatgttcccg gaatttggtg gcgattcctc ggcgcagacc
      481 gacaacttta tgaccaaacg cgccagcggt ctggcgacgt atcggaacac cgacttcttc
      541 ggcgttatcg atggcctgaa cttaaccctg caatatcaag ggaaaaacga aaaccgcgac
      601 gttaaaaagc aaaacggcga tggcttcggc acgtcattga catatgactt tggcggcagc
      661 gatttcgcca ttagtggggc ctataccaac tcagatcgca ccaacgagca gaacctgcaa
      721 agccgtggca caggcaagcg tgcagaagca tgggctacag gtctgaaata cgatgccaat
      781 aatttttatc tggcaacttt ttattctgaa acacgcaaaa tgacgccaat aactggcggc
      841 tttgccaata agacacagaa ctttgaagcg gtcgctcaat accagtttga ctttggtctg
      901 cgtccatcgc tgggttatgt cttatcgaaa gggaaagata ttgaaggtat cggtgatgaa
      961 gatctggtca attatatcga tgtcggggct acatattatt tcaacaaaaa tatgtcagcg
     1021 tttgttgatt ataaaatcaa ccaactggat agcgataaca aattgaatat taataatgat
     1081 gatattgtcg cggttggcat gacctatcag ttttaatgat tattgccgga tgtgatgcat
     1141 ccggcacatt tcactcacgc cgttaacttc accggctcgt cacgaaaatc atccgccggt
     1201 tccagcttca gattcagcgt cgtcagcagt tcacgcagct tctcgtgaaa ctcacgcagg
     1261 gtgtgctcca gtcgttcaac cacttcagtg tcttttaccg gaacactctt ccagtcgcct
     1321 gctttatcga acagaccaaa ctggtaactg taggtaaaac gggattcctg cgcttcaagc
     1381 tccatccacc agccccagaa ctcacgcact tccggtgccg gtttcacgtt gacgcataca
     1441 gccagacaat cgaaaaagaa tcgattatct tcgcacttac cttcacgaat atacgggcct
     1501 agtgcggtaa attttttgat caatctgctc ttcgggtgtc cactcggtaa cgtcattgct
     1561 atctcctgtt gtgatgcaac tgttttacca aattggcaaa atttagcaaa tttttaacac
     1621 aagcgttttt cgatccagcc ggtgatttcc tgaagacctt tgtcaaaatt ccgatacacc
     1681 gggttaaatg ggatctctaa taatttaccg tcagcagatg atgaggtgat taagcgtgag
     1741 tcctcttccg ggctgaacgg atcgttcttc cagtagcctg ataacattgg cgttgggcag
     1801 cgacgcccca gcaatccttg cacttttaat gaatagcgat tcagctccac gcgtaacgcg
     1861 tcatcggaag catcatgcat ccccaaacga ctcgccagaa cgtcaagata catttccggc
     1921 acctgttgct ggcacttaaa atcactcaac agggtatgaa ctaccggacc aagacaggca
     1981 accgctttca gacgcggcga ttcaagatat gccagacgca cggcaacgtt agcgccgaaa
     2041 cggaaaccaa aggccgcgac gcgagtgtga tccacccacg gtacgttagg cagcgccttt
     2101 aagacgtgct gatgcaacag gctggagtcc tgggtgagct tccattttga agaaaagccc
     2161 accgatggca tatcaatagt cagcatcgca atgccgcgcg gcgcaaaata acgttcatac
     2221 aggctgtaat agtccgtctg catcgcatcc agaccaccac acattaatac tgtcgggaac
     2281 gggccatcgc ctttcggcat atgcaaaaag ccggtgatgg gcgcaccgcc gggtacggta
     2341 aactccatct gccgcatcgt gcccggcaga cgctgggcgg cctcttcata ggcgcggttt
     2401 gataaagcct gcgcttgctc ggccaggtca tctcctttca gatgaggata ggcggcaatg
     2461 ttgtacaacg tagccgcatg cagccagtgt cgaccactga gttgcggatc ttcttccgca
     2521 caggcttttt gttgccacac catcgcctgg gtggcccact cataaatcca gttgccacca
     2581 cggtagccaa tcacagtatc gtataaatca tcgtcggtac gttcagcatc gctcatcaca
     2641 atacgtgcct ggacgtcgag gatttcacgt gggtcaatac cgcgccagat ccacatcaga
     2701 cggttaatca tgcgatacca gtgagggatg gttttaccat caagagccga ctgcacaggc
     2761 ggttgtgcgc cgtgattaaa gcggcggact agcgtcgagg tttcaggatg tttaaagcgg
     2821 ggtttgaaca gggtttcgct caggtttgcc tgtgtcatgg atgcagcctc cagaatactt
     2881 actggaaact attgtaacct gcctgaagtt aaaaagaaca acgcccggca gtgccaggcg
     2941 ttgaaaagat tagcgaccgg agattggcgg gacgaatacg acgcccatat cccacggctg
     3001 ttcgatccag gtatcttgcg ggatatcaac aacatagtca tcaaccagcg gacgaccagc
     3061 cggttttgcg aagatggtga caaagtgcgc ttttggatac atttcacgaa tcgcaacagc
     3121 agtaccaccg gtatccacca ggtcatcaat aacgatgaag ccttcgccat cgccttctgc
     3181 gcgtttcagc actttaagct cgcgctggtt gtcgtgatcg tagctggaaa tacaaacggt
     3241 atcgacatga cgaataccca gttcacgcgc cagtaacgca cccggtacca gaccgccacg
     3301 gcttacggca ataatgcctt tccattgttc agaaggcatc agtcggcttg cgagtttacg
     3361 tgcatggatc tgcaacatgt cccaggtgac gatgtatttt tcgctcatgt gaagtgtccc
     3421 agcctgttta tctacggctt aaaaagtgtt cgaggggaaa ataggttgcg cgagattata
     3481 gagatctggc gcactaaaaa ccagtatttc acatgagtcc gcgtcttttt acgcactgcc
     3541 tctccctgac gcgggataaa gtggtattct caaacatatc tcgcaagcct gtcttgtgtt
     3601 gacaacattt tctgctaacc ctgtgacctg caatactgtt ttgcgggtga tcgacaagga
     3661 gacttaacgt gtctgaactg tctcaattat ctccacagcc gctgtgggat atttttgcca
     3721 aaatctgttc tattcctcac ccgtcctatc atgaagagca actcgctgaa tacattgttg
     3781 gttgggcaaa agagaaaggt ttccatgtcg aacgcgatca ggtaggtaat atcctgattc
     3841 gtaaacctgc taccgcaggt atggaaaatc gtaaaccggt cgtcttacag gcccacctcg
     3901 atatggtgcc gcagaaaaat aacgacaccg tgcatgactt cacgaaagat cctatccagc
     3961 cttatattga tggcgaatgg gttaaagcgc gcggcaccac gctgggtgca gataacggca
     4021 ttggtatggc gtctgcgctg gcggttctgg ctgacgaaaa cgtggttcac ggcccgctgg
     4081 aagtgctgct gaccatgacc gaagaagccg gtatggacgg tgcgttcggc ttacagagca
     4141 actggttgca ggctgatatt ctgattaaca ccgactccga agaagaaggt gaaatctaca
     4201 tgggttgtgc ggggggtatc gacttcacct ccaacctgca tttagatcgt gaagcggttc
     4261 cagctggttt tgaaaccttc aagttaacct taaaaggtct gaaaggcggt cactccggcg
     4321 gtgaaatcca cgttggcctg ggtaatgcca acaaactact ggtgcgcttc ctggcgggtc
     4381 atgcggaaga actggatctg cgccttatcg atttcaacgg cggcacactg cgtaacgcca
     4441 tcccgcgtga agcctttgcg accattgctg tcgcagctga taaagtcgac gtcctgaaat
     4501 ctctggtgaa tacctatcag gagatcctga aaaacgagct ggcagagaaa gagaaaaatc
     4561 tggccttgtt gctggactct gtagcgaacg ataaagctgc cctgattgcg aaatctcgcg
     4621 atacctttat tcgtctgctg aacgccaccc cgaacggtgt gatccgcaat tccgacgtgg
     4681 caaaaggtgt ggtcgaaacc tccctgaacg tcggtgtggt gaccatgact gacaataacg
     4741 tagaaattca ctgcctgatc cgttcactga tcgacagcgg taaagactac gtggtgagca
     4801 tgctggattc gctgggtaaa ctagctggcg cgaaaaccga agcgaaaggc gcatatcctg
     4861 gctggcagcc ggacgctaat tctccggtga tgcatctggt acgtgaaacc tatcagcgtc
     4921 tgttcaacaa aacaccgaac atccagatta tccacgcggg cctggaatgt ggtctgttca
     4981 aaaaaccgta tccggaaatg gacatggttt ctatcgggcc aactatcacc ggtccacact
     5041 ctccggatga gcaagttcac atcgaaagcg taggtcatta ctggacgctg ctgactgaac
     5101 tgctgaaaga aattccggcg aagtaattat ttgatttgct gccggatggc gtttaatcgc
     5161 cttccggcag tttcatcctt cattatcctt cgataaaagc catccctgta aatgtccgtc
     5221 tcgggttgcc acgttcaatc tggtgatgga acattcgccg cagcgatttc agcaccgcgc
     5281 tattttcctg ttgctgctgc tccagcttcc aggcaatcag caatcgtgcc agccgcttgt
     5341 tagcatgctg actacgctct gactgaacct tcacgctaat accggatgcc aaatgcgtgg
     5401 cgcgtaccgc cgagtcggtt ttattgacat gttgaccgcc cggccccgac gaacgcaacg
     5461 tctcataacg gattgcatcc gattgttcct gctcatcagc ggtaaaacgc ccaatgccca
     5521 gaaaccagtt tttgcgccca tgatgaggcc gatacggact cggacaaatc cactgaatag
     5581 tgccacacca gctttcgctt aatgcccatg cgttatcgcc atccagagaa atcagcgccg
     5641 aacgcagcgt gtcagagtag tggcccgttt ctgtttccag caccgttacc gcgacgtctt
     5701 gtcgggcagc ttctttaatt agcctgtcca gcgccttttt cactgcgaga caacattctt
     5761 ccggcccctg cgctgaggag agttgtagca agatcatcct tttttccctc cactgttttt
     5821 gagcgttagc accggacgca gccgcgcgac aggaataatt aaaccggcct gcaccagaca
     5881 ttgcaccacg ctttcagccg atttataggc ttgtggcgct tcttcaaaga tgagttgttt
     5941 atcgcgacaa attacccgac tgccaagttc agtacgcgag agttgcgtcg ccgtgtattt
     6001 cgctgccaga cgccctttac actcggtgcg cccccattta cgccctgccc catgcgccag
     6061 cgagtggagc gttttttcgt tcgcaacagg ctgtacaatc caggagtaat caccacgtga
     6121 accgggaata atcaccagac cgttgtcatc cggcgtggca cctttacgat gcaaccagcc
     6181 ctgttgatcg ccgatttgac acgcgctaac aaagttatgc gccacatcca gaaccggact
     6241 tcccgtggcc ttaacttgtt gcattatgcg caaagcgatc atctggcgat taatacacgc
     6301 aaacgccagc gcatcgtcat gttccgcaat ataccgtagc gcgtcgtcac tgccttgagg
     6361 caaaccatga tgcgagaacg aggcaatatg ccgctgtaaa atagactgcc ccaccccccg
     6421 cgagccgcta tgaaccaaca gttgcagatg ttgtgcatcc agacctgcca gtgcaaacag
     6481 ttcagcgttg atgatttgat caacttgttg cagttcagcg aaatgattgc cgccaccgat
     6541 agagccaagc gagctgcgcc acggatgctg tgcaaatgct gacggcaggt tttcctccag
     6601 ccagctttct tcagcaacgt catccagatc tgataatcgc ttttcaaact tatcggcgtt
     6661 gtatttgcga gcgagaatat ctgtttgcca tagcgccata ccgcagccga tatcattgcc
     6721 gaccagtgcc ggataaaaac gaccaacaga gaagaacgct gcgccaatcg gatagccgcg
     6781 tccgggatgt aaatctggca tccccactac gcgctgcatg ttgggtaaat ttgccgtggt
     6841 gtgtaattgt tggatcgcta aactctcgat ccacaggtca tcagatgcga tggtaaatac
     6901 cgcatcggat aagggacgaa tataattgcc catataccat tccgtaaata gactcgtcat
     6961 acttcaagtt gcatgtgttg cgacttgaat tattgagagt aacaattaaa aaatcaggaa
     7021 atggcagacg aatccagggc aagaaaaaaa gaattaatcc tggaaaggtc tgcaaagagt
     7081 cattgataaa ggatttgtca tgatttacct ccttgcagtt tgtggaataa gaaaacgcgc
     7141 acatactagg cttgcgttat tttcattgca agctggattt aatgttgctg tttataacgc
     7201 atataaaaat taataaacca ctctccccga cattcaacgc gttgctgctt aactgtctga
     7261 aaaccataac gttcaaaaaa gggttttgcg gttatgcttg cgtccaccgt aagttcggat
     7321 tcagacttaa tcaaaggttt taacaaagcg ctggcaaccc cacggcgggt gtattcaggg
     7381 tcaacaaata acatatcgat atagtgttca acgcaggtaa taaagccgac cagttttgca
     7441 ttaatgactg caacccgcac ttgtgatttc gcgagtttct ccttccagcg agattcgtca
     7501 atctgcgccc aggcggcaat ttgttgtggt gaataatgct gactggcagt catcgtaacc
     7561 gctctaatga aaatagcgca tagttgctga aaatcgccag gctgatagtc tcttatttgt
     7621 atgttattca taatcccagc accagttgtc tttccatttg cgggtcaagc aacgtcacat
     7681 gcagccccac cagtcgcaca ccgcgcccgc cgcggcgttc atcccaggtt ttacgcgcgg
     7741 tggcgattaa gtcagcttta ttcagccgcg gccagacgtg ctcctgagtg gtttgctgaa
     7801 aatcatcaaa ctttaatttc actccctgac gggcgatcag taaatcaggt tttacctttg
     7861 ccagacggcg ttcaagttcc ggatacagcc gctcgataat cgcttcacat tcagaccagt
     7921 gatgaatatc ttccgccatc gtgcgttcca cgccgacgga ttttcgtaac cgttcgctgt
     7981 taacgtcgcg ctcgtcaatc ccctgactac gctcccacaa aatgcggcca aatttgccaa
     8041 agcgtttaag cagcatcacc agatcacact tttgtacatc accgcaggtc cgcagcccca
     8101 tcgcttccag ttttgccgcc gagactttgc cgacgccggg gatttttgcc agcggtaagg
     8161 tttgtaaaaa tgccggaact tctgccggcg taatcacaaa ctgaccgttg ggtttattca
     8221 tgtcggaggc gattttggcg agaaacttta ctggtgccac gcccgcagac gctgtcagtt
     8281 gcagctcgct gaaaatcgtc tggcggattt cctgggcgat gagggtcgca gaaccgtggc
     8341 aatggacgct atcggtgacg tcgagataag cctcatccag tgacaacggt tcaatacgcg
     8401 aggtgtagcg cgagaagatt tcacggatat gatttgaggc ttctttgtag gcgtcaaagc
     8461 gccccggaag caaggtgaga tgcgggcata atttgagcgc catccctgtc ggcatagcgc
     8521 tacgtacgcc aaatttacgc gcgggataat tggcggtgct gatcaccccc cgacgttcgc
     8581 ggctgccgcc aatagcaata gggatatcgc gcagggcggg attgtcgcgc atctccaccg
     8641 ctgcgaaaaa gcagtccata tccacatgaa tgattttacg cattactcac ctctcaacac
     8701 tggtaaagta tacagtgatt tcagggtttg agaaatgcgt aaagattcag catttaacgc
     8761 gttcgctgcg aaagcacctg ctgcttatcc agcttttgca cctgcggctg caccacttta
     8821 tccccatgct gaccaaagta ttgatacagc gtatcggacg cacttttggt cagcaccata
     8881 atctcaatgc gccggttgcc cgcgctttgc ggatttttgg aatccagcag catctggtcc
     8941 gccattgcgc ttacctgcat cactttatct tccggcattc cggcctcttc cagcacccga
     9001 cgagccgaaa gcgcgcggtc acccgaaagg ttccagttgt tgtagatatt gtttttgtaa
     9061 gccatcgcat cggtatgccc ggtaataata attttgttat cgagcgagtc gaaaactggc
     9121 gcgagctcca ccagcagcgt tttaaagaac ggcataatct gcgcgctgcc gcgttcgaac
     9181 atattgcgat tctggtcgtc tttaatcagt acgcgtaatc cctggggaac aatctccatc
     9241 tccagattcg cttccatatg cgcatcacgg gcaatggtgt tgatactggt tgccagctcg
     9301 ccaagctcgg tggcggattt cttcttcagc agcgccgttt tctcattcac atcacgcgct
     9361 tttttctccg tctcttccgg caccgccacg gtggcaggtt ttggcggatg agacgggctg
     9421 attttattta acggcgacag cccgccaccg ttaaaaatcg actgcccgtg cagcgcggcg
     9481 ataatgcttt cacgctcgga tttactgacg ctgttgacaa tccacagcgt cataaacagc
     9541 gccatcatcg ccagggtaaa gtcggcgaac gccaccttcc acgccccgcc atgatgcccg
     9601 gcatgattct ttttgatctg ccgccggacg atggtggttt ttgccccacg ctcgcggcgg
     9661 ttgcccgtct ttctcatgct tattcttgct ccagcatcgc attcacccac gcgtcgaggt
     9721 tggcgaaggt cggtttggac gccagatgca gcagcttacg cccggcatcc accgccagca
     9781 gcgtcggttt gccgcctgcc tgcgccacca gcacagtgcg cacgcattcc agcaaagaat
     9841 gttctgcccg cgcctgttgt tccatggcgt tggcgagcgg gtccatcagg cagtagcaga
     9901 taaagacgcc taaaaacgtc cccaccagcg ctgccgccac tttcaggccg atcagggcaa
     9961 tagagccgtc aatcgactgc atggtgatga tgatccccag cactgctgca caaataccaa
    10021 atcccggcat cgcttcggcg gtacgttgca gcgagcgcga cggcgtgagc agcgactctt
    10081 ccgcagtgtc cagttcctga tcgagaatgc cttccagttc atgggcgtcg attttaccca
    10141 tcgccatcag gcgaaagtta tcggcaataa aggtcaccag ccgcttctgg gttaacacca
    10201 gcgggtattt ctggaaaatg gtgctctctt ccggttgctc aatatgttga tccagcatgc
    10261 gcagaccacc gttttgcacc atttcgagca actcatacag gcacattaat agctgacgct
    10321 gaaactccgg cccgagctgt tttttactga tcacaccttt aatttgatgg gcaatctctt
    10381 tcagcacatg tttcgggtta ccaataatca tcgccccgaa accggcaccc aaaataatga
    10441 tcatctccgc cggttgccag atagccacta attgcccgcc cgcttcaaaa taaccgccaa
    10501 atacgcagcc gagcaccacc agtaaaccta atattttttg catgatttct tcactttaat
    10561 tcggatagac aaaatcacga attttctgcg cgatctgttt attcagttga caaattcggg
    10621 cctcggttag ccctaatacc agcgcaatct ctttgagatt catctcatgc tggtaataca
    10681 tggagaggat cagttgctct ttttcgctta attgtgaaag cgcctgttgc agcatttgct
    10741 gagtgacaaa ctgatcttcc agctcccggc caacaagcgg aatgcctggc ccttcgccgt
    10801 tcagcagttc gtccaggctg gcgagcgttt ccgccccttc cagttgctga tattcgagat
    10861 aatcttcatg tgagatccct tccacggcca gttctgacca ctccggttcg tgccccagtt
    10921 ttttgcgcgt ttcgcgaatc agatctttca tctgatgata tttctgtcgc agctggcgcg
    10981 gtcgccagtc gagagcgcgc agctcatcca ggatcgcgcc gcgaatacgg tgcacggcat
    11041 agcccgcaaa accttcgtcc ggcagcccgt agcggcggat ggcattaagc agccccatta
    11101 acgccgtttg ttccatatcc tgacgatcaa taatgcaatt acactgcggg gcaagttgcc
    11161 gcaccacttt acgcaccaac ggtaaatagg cctgcaagta gtggctctcc tgctgcgggg
    11221 ttaataccgg cgcggcagca aattcttctg actcgaatac atcctgtagc atgtttatat
    11281 ccgcctggtg attactggaa gaccactttt ttcaccagca aatccttata cggtgcgctc
    11341 attttgcgac gctgaagatc tttctttaac gcgtcagaaa tgagtttgcg gatggcagat
    11401 attttcatgc cgcgcaaatc ttcaaatttc atttccgtca ggcattcaac cgcaatactt
    11461 tgatacagcg gctcctgatg ttttattttt tccgcttctt tgtcactggc gaccaccatc
    11521 actaattccg ccgacaaata atggtccgcg ccgttgttgt catgcagcgt gacaatggtt
    11581 tccggcaacg tcacaaacac gcttttgctt tcatccaggg tttccaccgt ttccgtctgc
    11641 gcggcggcag tcggttttcc ttttccggca taatgatgcc agacacccca tccagctccg
    11701 gcccccacca ccaacgccag aacggcgctg accacgcccg ccaccacgat tttcttcata
    11761 ttgcctttac actttgatca aaacggattc gttctgttga aattgaggcg catcatcaag
    11821 ctgtaaggcg gcactcacct cttcctgctg atggcccgac tgttgttgtt gctgcgactg
    11881 cccttcggag gaaacctgta cgttcacctc cataaaattt tgcgccgtca gatgctggcg
    11941 gagatcgtcg ctaaactgct gtaacgcgcg gcaaacttca ctttggttcg cgccgatgtg
    12001 caccatcagt ttgccgttgt cgagttgtac ggcgatctcg agcttgccaa gcgacggtgg
    12061 atcgagacgg atggtggaga tctgctgttg tttgttgagc tgaaagtgga tctgatcttt
    12121 caaaagcgtc gtcagttttt cgcccagttc gtccatcgaa agcgtggctg gtttggcgac
    12181 aatctcctga tgatcgctga gcagctccgg cgtcttaacc tgcaacaccg cacgcgcaat
    12241 aaccggttca ctgtccggca ctttttccgt tttacgctcg acctgcgggc gcgcggtcac
    12301 acgggttagt tcagatttcg ggcgagctgg ctgtgttgag acgcgcggct gcgtcggcgt
    12361 aatggcatgt aaatcttcac tagcgtaagt cgccagtctg gcctgctgtt cgggcttcgc
    12421 ctgcggcaac tgactgatca gttcctgcaa ctgcggcggc aacggcgtga gttcctgctg
    12481 ttgcgtctgg cgttgcggcg cgttttgcac caccgctttc gtcaattgct ggataaccgg
    12541 cgatgttgcg acgtttcgcg gctgcggcgt gtcctgatgc ggcgcggcag gttgcggaag
    12601 taacagcgcc attagcgcct gcatcgccac cggatcgttc tcctgcgggc gcgtcgcctg
    12661 ttgctgactg gtttttgcgc tatgaacgcg ctccgccacc gccgcgaccg ccattttcgg
    12721 cagggtaaac gcgggcgcgt tttcaccgct caccggcggt aaaatatccg ccttcaacga
    12781 agcagtttcc gcaagcgttc cgagcgtggc aagcaatgtc ggattcatga cttctccgaa
    12841 agtaaattaa caattgagta ggccagtacg ccttcctgat gccgctgatg ctgttccata
    12901 cgcgctttca acacttctgc gcgctgctcg cgccgtttaa tcagggcgtt aaacgcgtca
    12961 tacagtcgcg ctttcactgc atccaactgc gccgcatcgg tttccgccgc cgcgtaatcg
    13021 tgaaactggc gtatcaactg ctgatgcagt gccggaaggc gtcgccagcg ttgcttgtcg
    13081 agggcttcat tcatcgcctc gaccagtgcg aaaagtttcc tgcgttgttt ttccatcgtc
    13141 ttaacccagc ttgcccgaaa gcccttccca gccttcgcgc aggttgctta agatcagcat
    13201 cacttcatcg attttctctg ccgacagttc gccgctggcc tcatacaatc ggtaaacgca
    13261 gtggtcatag agtcgcgaaa ggttgaccac cagctcgccg ccagtttcaa actccagcga
    13321 actggtgagc gcattgagaa tgtcgatgca tttattgatg ctctgggctt tcttctcgaa
    13381 acgccgccct tcaatatggc ttttggcgcg ttccagttcg tccatcaggc cagaaaacag
    13441 caccagcacc agttccagag gcgatgccgc cgcagtacgc gccgcgagat cgatctcttt
    13501 atattgcgaa tagccgtcct gtacttggta catctttagg gatattcctt ctgagttacg
    13561 cgaatgccgc catgctggct ttcatggtgt acacctcgac cagcgtgttg gtgtactcct
    13621 cgagataacg gtcgtagttg gcgttatagg tatcagtaag ctgatcgcct tcgtcctgaa
    13681 ttttgctcat ctgatcgtca atgttctgct ggcgcagggt gatgatgccg ttgctggagt
    13741 ccgtgtaggt gttaataagg tcgtccatct gcgccaccat gctgttgtcg ccaacgaaaa
    13801 tagaggtcag accgtcagga tttttcgcca tttcgtcgtt gaactggtcg gagtcaatct
    13861 gtaagtggcc gtgagaatcg agggtgatgc catagtcaac aatcgacacg ccgttgtagc
    13921 tggcatgggc gatgtcatcg agctggttcg ccagtgaact gagtccggcg tcgcctgcaa
    13981 ataccccggc gctggtactg tcatcaccgt gggtggttag cgaatcaacc gtatcaatca
    14041 aggtgttata ggcatcgaca aaggtctgta ctttctcctg gctggcgctg gaatcttcgc
    14101 tgatgttaaa ggtagtgaga tcgctgtcag aatcgctgac ttcggtgaaa gtcatggtga
    14161 caccgggaat cacatcatca aacgtattgc tgctgttggt gatctcaggc cccgtggcgc
    14221 tgccaagatg aataatcgcg tcctgagcgg aggaaacatc cgtggcaacg ggcgcgctgg
    14281 tgctatcgtt gctggcatca tgccccgtta cgctcaccga aaacgcgctc tgcgccccgg
    14341 tgctatccga ggtgagcata atcgttgtgg tgccgtcggt ttttaccagc gccgccgaca
    14401 cgcccggatt atcatcggag tcgttaatcg cattcaccag ttcactggcg tcgataaaac
    14461 cgtcgccgtc accattctga tctgccgccg acagatcgat atccatggtg ctgtcgccca
    14521 tcgtcagttc aaaagtgccg gtggcggaaa aggcgtcgtc gcccatactg aacgtggttt
    14581 gctgcccctg tgccagttgc tcaacgaaaa atgagtagct tcccgcctgc gcctgggaat
    14641 tggcggaaac gctcgccgag tcattattgc tggtggcggc aaacgtcacc gggccgtcgg
    14701 tatcgctgtt cagcgcgtca acggcgctct gaaaatcgct caacgctgaa ctgagcgaat
    14761 ccaggccgct gctttcagca tccagctctg tttgcttctc ctgcaagttt gccgcctgag
    14821 tggcgacatc cgcatacgcg atttcttgcg caatggttct tgggttgatc attgatgact
    14881 ccggataacg atgtcaggtt attggcaaaa ggcgtgccaa tattttttat gtgaataatc
    14941 aatagattaa aaaacaggcg gaaatggcgt ttccggtctg gcggaaaaaa gcctgccgca
    15001 gaaagcacaa caccgcccgc aggcggtgtt gttcgtagtc cgaataaggc gtttacgccg
    15061 catccggcaa gtccgccatt actgcagcag gctggaaacc atgctggaca tgctgttgga
    15121 ctgtttcagc atggtgatgc cagtctgcat cagcacctgc tgtttggtca tattggacgc
    15181 ttccgtcgcg tagtcggtat ccataatgtt accgatagcg acttcggtgt tgtcctgcat
    15241 actggtcagg ttgttggcgg tgtcgttcag acggttgata gacgcaccca gcttggactg
    15301 gatctgcgaa acgtcatcca tcgcggtaga gatgctggtg atcatctggt tagccgagcc
    15361 agacgcggtc agctcagtac cgccagaaac acctgcagta cccgtggtgt ctgcctgatc
    15421 cgcgctaaag gagttgctga tagcagaaag atcggtcacc agcgtgttca gttggctgga
    15481 gatgttcacg gtcatggtgt cggaagattc cgcgcccacc tggaaagtta ccgcgctctg
    15541 gaacagaccg tcagtaccgg tgttgctggt accggaaaca ccgaacaggt tggtgccgcc
    15601 gtaagtggtg ttttgcagca tgtcagacat ctgctgaccc agttcgtcgt attcgtcctg
    15661 catcgcctgc agatcgtcgt cgctgtaggt gccgttcgct gcctgagttg acaggtcttt
    15721 catacgaccc agcacatcag acatttcatc gaacatacta tcggcggttt gcagcatcgc
    15781 ggttgcgtca ttgatgttgc tcagcgccac gcccataccg ctggactgtg ctgtcagacg
    15841 gttagcaatt tgtttgcctg cggcgtcatc ggcagaagag ttaatccggt tacctgtcgc
    15901 caggcgttcc atagatgtag acaaagaaga gctgcttttg ctgattgcat taacggcggc
    15961 cattgaggcg ttattggtgt taatagataa catgatactg ctccttggga ttaatcgttt
    16021 tcgtttcgat accctgtaaa aacgacacct aaggagcaaa cttaaaaccg ccgttaaaaa
    16081 aatttcactg cgatttatct gatgccactt ttagctaaaa aaagcagagt ttaaattcag
    16141 ataaaa
//
LOCUS       DAZKHF020000087        16083 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2201S31241
            SAMN43267800-rid25106413.denovo.088, whole genome shotgun sequence.
ACCESSION   DAZKHF020000087 DAZKHF020000000
VERSION     DAZKHF020000087.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267800
            Sequence Read Archive: SRR30307263
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 16083)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16083)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 16083)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350581.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350581.2
            Genome Coverage       :: 151x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:21:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,652
            CDSs (total)                      :: 5,557
            Genes (coding)                    :: 5,320
            CDSs (with protein)               :: 5,320
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 237
            CDSs (without protein)            :: 237
            Pseudo Genes (ambiguous residues) :: 0 of 237
            Pseudo Genes (frameshifted)       :: 102 of 237
            Pseudo Genes (incomplete)         :: 130 of 237
            Pseudo Genes (internal stop)      :: 52 of 237
            Pseudo Genes (multiple problems)  :: 41 of 237
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16083
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267800-rid25106413.denovo.088"
                     /serotype="O 26:H11"
                     /isolate="2201S31241"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-01"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            212..1111
                     /gene="yegS"
                     /locus_tag="ACHOSC_RS11640"
                     /old_locus_tag="pgaptmp_002328"
     CDS             212..1111
                     /gene="yegS"
                     /locus_tag="ACHOSC_RS11640"
                     /old_locus_tag="pgaptmp_002328"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0001727 - lipid kinase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipid kinase YegS"
                     /protein_id="HGU4548258.1"
                     /translation="MAEFPASLLILNGKSTDNLPLREAIMLLREEGMTIHVRVTWEKG
                     DAARYVEEARKLGVATVIAGGGDGTINEVSTALIQCEGDDIPALGILPLGTANDFATS
                     VGIPEALDKALKLAIAGNAIAIDMAQVNKQTCFINMATGGFGTRITTETPEKLKAALG
                     GVSYIIHGLMRMDTLQPDRCEIRGENFHWQGDALVIGIGNGRQAGGGQQLCPNALIND
                     GLLQLRIFTGDEILPALVSTLKSDEDNPNIIEGASSWFDIQAPHEITFNLDGEPLSGQ
                     NFHIEILPAALRCRLPPDCPLLR"
     gene            complement(1193..1966)
                     /locus_tag="ACHOSC_RS11645"
                     /old_locus_tag="pgaptmp_002329"
     CDS             complement(1193..1966)
                     /locus_tag="ACHOSC_RS11645"
                     /old_locus_tag="pgaptmp_002329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020077849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR family DNA-binding transcription
                     regulator"
                     /protein_id="HGU4548259.1"
                     /translation="MNSFERRNKIIQLVNEQGTVLVQDLAGVFAASEATIRADLRFLE
                     QKGVVTRFHGGAAKIMSGNSETETQEVGFKERFQLASAPKNRIAQAAVKMIHEGMTVI
                     LDSGSTTMLIAEGLMTAKNITVITNSLPAAFALSENKDITLVVCGGTVRHKTRSMHGS
                     IAERSLQDINADLMFVGADGIDAVNGITTFNEGYSISGAMVTAANKVIAVLDSSKFNR
                     RGFNQVLPIEKIDIIITDDAVSEVDKLALQKTRVKLITV"
     gene            complement(2072..3112)
                     /gene="gatD"
                     /locus_tag="ACHOSC_RS11650"
                     /old_locus_tag="pgaptmp_002330"
                     /pseudo
     CDS             complement(2072..3112)
                     /gene="gatD"
                     /locus_tag="ACHOSC_RS11650"
                     /old_locus_tag="pgaptmp_002330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310921.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="galactitol-1-phosphate 5-dehydrogenase"
     gene            complement(3160..4515)
                     /locus_tag="ACHOSC_RS11655"
                     /old_locus_tag="pgaptmp_002331"
     CDS             complement(3160..4515)
                     /locus_tag="ACHOSC_RS11655"
                     /old_locus_tag="pgaptmp_002331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015577 - galactitol transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0015796 - galactitol transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS galactitol transporter subunit IIC"
                     /protein_id="HGU4548260.1"
                     /translation="MFSEVMRYILDLGPTVMLPIVIIIFSKILGMKAGDCFKAGLHIG
                     IGFVGIGLVIGLMLDSIGPAAKAMAENFDLNLHVVDVGWPGSSPMTWASQIALVAIPI
                     AILVNVAMLLTRMTRVVNVDIWNIWHMTFTGALLHLATGSWMIGMAGVVIHAAFVYKL
                     GDWFARDTRNFFELEGIAIPHGTSAYMGPIAVLVDAIIEKIPGVNRIKFSADDIQRKF
                     GPFGEPVTVGFVMGLIIGILAGYDVKGVLQLAVKTAAVMLLMPRVIKPIMDGLTPIAK
                     QARSRLQAKFGGQEFLIGLDPALLLGHTAVVSASLIFIPLTILIAVCVPGNQVLPFGD
                     LATIGFFVAMAVAVHRGNLFRTLISGVIIMSITLWIATQTIGLHTQLAANAGALKAGG
                     MVASMDQGGSPITWLLIQVFSPQNIPGFIIIGAIYLTGIFMTWRRARGFIKQEKVVLA
                     E"
     gene            complement(4519..4803)
                     /gene="gatB"
                     /locus_tag="ACHOSC_RS11660"
                     /old_locus_tag="pgaptmp_002332"
     CDS             complement(4519..4803)
                     /gene="gatB"
                     /locus_tag="ACHOSC_RS11660"
                     /old_locus_tag="pgaptmp_002332"
                     /EC_number="2.7.1.200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS galactitol transporter subunit IIB"
                     /protein_id="HGU4548261.1"
                     /translation="MKRKIIVACGGAVATSTMAAEEIKELCQNHNIPVELIQCRVNEI
                     ETYMDGVHLICTTAKVDRSFGDIPLVHGMPFISGVGIEALQNKILTILQG"
     gene            complement(4834..5286)
                     /gene="gatA"
                     /locus_tag="ACHOSC_RS11665"
                     /old_locus_tag="pgaptmp_002333"
     CDS             complement(4834..5286)
                     /gene="gatA"
                     /locus_tag="ACHOSC_RS11665"
                     /old_locus_tag="pgaptmp_002333"
                     /EC_number="2.7.1.200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS galactitol transporter subunit IIA"
                     /protein_id="HGU4548262.1"
                     /translation="MTNLFVRSGISFVDRSEVLTHIGNEMLAKGVVYDTWPQALIARE
                     AEFPTGIMLEQHAIAIPHCEAIHAKSSAIYLLRPTNKVHFQQADDDNDVEVSLVIALI
                     VENPQQQLKLLRCLFGKLQQPDIVETLITLPETQLKEYFTKYVLDSDE"
     gene            complement(5296..6558)
                     /gene="gatZ"
                     /locus_tag="ACHOSC_RS11670"
                     /old_locus_tag="pgaptmp_002334"
     CDS             complement(5296..6558)
                     /gene="gatZ"
                     /locus_tag="ACHOSC_RS11670"
                     /old_locus_tag="pgaptmp_002334"
                     /EC_number="4.1.2.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagatose-bisphosphate aldolase subunit GatZ"
                     /protein_id="HGU4548263.1"
                     /translation="MKTLIARHKAGEHIGICSVCSAHPLVIEAALAFDRNSTRKVLIE
                     ATSNQVNQFGGYTGMTPADFREFVFTIADKVGFARERIILGGDHLGPNCWQQENADAA
                     MEKSVELVKEYVRAGFSKIHLDASMSCAGDPIPLAPETVAERAAVLCFAAESVATDCQ
                     REQLSYVIGTEVPVPGGEASAIQSVHITHVEDAANTLRTHQKAFIARGLTEALTRVIA
                     IVVQPGVEFDHSNIIHYQPQEAQPLAQWIESTRMVYEAHSTDYQTRTAYWELVRDHFA
                     ILKVGPALTFALREAIFALAQIEQELIAPENRSGCLAVIEEVMLDEPQYWKKYYRTGF
                     NDSLLDIRYSLSDRIRYYWPHSRIKNSVETMMVNLEGVDIPLGMISQYLPKQFERIQS
                     GELSAIPHQLIMDKIYDVLRAYRYGCAE"
     gene            complement(6587..7441)
                     /gene="gatY"
                     /locus_tag="ACHOSC_RS11675"
                     /old_locus_tag="pgaptmp_002335"
     CDS             complement(6587..7441)
                     /gene="gatY"
                     /locus_tag="ACHOSC_RS11675"
                     /old_locus_tag="pgaptmp_002335"
                     /EC_number="4.1.2.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416599.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagatose-bisphosphate aldolase subunit GatY"
                     /protein_id="HGU4548264.1"
                     /translation="MYVVSTKQMLNNAQRGGYAVPAFNIHNLETMQVVVETAANLHAP
                     VIIAGTPGTFTHAGTENLLALVSAMAKQYHHPLAIHLDHHTKFDDIAQKVRSGVRSVM
                     IDASHLPFAQNISRVKEVVDFCHRFDVSVEAELGQLGGQEDDVQVNEADAFYTNPAQA
                     REFAEATGIDSLAVAIGTAHGMYASAPALDFSRLENIRQWVNLPLVLHGASGLSTKDI
                     QQTIKLGICKINVATELKNAFSQALKNYLTEHPEATDPRDYLQSAKSAMRDVVSKVIA
                     DCGCEGRA"
     gene            complement(7751..8803)
                     /gene="fbaB"
                     /locus_tag="ACHOSC_RS11680"
                     /old_locus_tag="pgaptmp_002336"
     CDS             complement(7751..8803)
                     /gene="fbaB"
                     /locus_tag="ACHOSC_RS11680"
                     /old_locus_tag="pgaptmp_002336"
                     /EC_number="4.1.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006820796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004332 - fructose-bisphosphate aldolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I fructose-bisphosphate aldolase"
                     /protein_id="HGU4548265.1"
                     /translation="MTDIAQLLGKDADNLLQHRCMTIPSDQLYLPGHDYVDRVMIDNN
                     RPPAVLRNMQTLYNTGRLAGTGYLSILPVDQGVEHSAGASFAANPLYFDPKNIVELAI
                     EAGCNCVASTYGVLASVSRRYAHRIPFLVKLNHNETLSYPNTYDQTLYASVEQAFNMG
                     AVAVGATIYFGSEESRRQIEEISAAFERAHELGMVTVLWAYLRNSAFKKDGVDYHVSA
                     DLTGQANHLAATIGADIVKQKMAENNGGYKAINYGYTDDRVYSKLTSENPIDLVRYQL
                     ANCYMGRAGLINSGGAAGGETDLSDAVRTAVINKRAGGMGLILGRKAFKKSMADGVKL
                     INAVQDVYLDSKITIA"
     gene            9060..10337
                     /gene="yegT"
                     /locus_tag="ACHOSC_RS11685"
                     /old_locus_tag="pgaptmp_002337"
     CDS             9060..10337
                     /gene="yegT"
                     /locus_tag="ACHOSC_RS11685"
                     /old_locus_tag="pgaptmp_002337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005337 - nucleoside transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015858 - nucleoside transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside permease"
                     /protein_id="HGU4548266.1"
                     /translation="MKTTAKLSFMMFVEWFIWGAWFVPLWLWLSKSGFSAGEIGWSYA
                     CTAIAAILSPILVGSITDRFFSAQKVLAVLMFAGALLMYFAAQQTTFAGFFPLLLAYS
                     LTYMPTIALTNSIAFANVPDVERDFPRIRVMGTIGWIASGLACGFLPQMLGYADISPT
                     NIPLLITAGSSALLGVFAFFLPDTPPKSTGKMDIKVMLGLDALILLRDKNFLVFFFCS
                     FLFAMPLAFYYIFANGYLTEVGMKNATGWMTLGQFSEIFFMLALPFFTKRFGIKKVLL
                     LGLVTAAIRYGFFIYGSADEYFTYALLFLGILLHGVSYDFYYVTAYIYVDKKAPVHMR
                     TAAQGLITLCCQGFGSLLGYRLGGVMMEKMFAYPEPVNGLTFNWSGMWTFGAVMIAII
                     AVLFMIFFRESDNEITAIKVDDRDIALTQGEVK"
     gene            10334..11338
                     /gene="yegU"
                     /locus_tag="ACHOSC_RS11690"
                     /old_locus_tag="pgaptmp_002338"
     CDS             10334..11338
                     /gene="yegU"
                     /locus_tag="ACHOSC_RS11690"
                     /old_locus_tag="pgaptmp_002338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-ribosylglycohydrolase family protein"
                     /protein_id="HGU4548267.1"
                     /translation="MKTERILGALYGQALGDAMGMPSELWPRSRVKAHFGWIDRFLPG
                     PKENNAACYFNRAEFTDDTSMALCLADALLEREGKIDPDLIGRNILDWALRFDAFNKN
                     VLGPTSKIALNAIRDGKPVAELENNGVTNGAAMRVSPLGCLLPARDVDSFIDDVALAS
                     SPTHKSDLAVAGAVVIAWAISRAIDGESWSAIVDSLPSIARHAQQKRITTFSASLAAR
                     LEIALKIVRNADGTESASEQLYQVVGAGTSTIESVPCAIALVELAQTDPNRCAVLCAN
                     LGGDTDTIGAMATAICGALHGVNAIDPALKAELDAVNQLDFNRYATALAKYRQQREAV
                     "
     gene            11335..12300
                     /gene="yegV"
                     /locus_tag="ACHOSC_RS11695"
                     /old_locus_tag="pgaptmp_002339"
     CDS             11335..12300
                     /gene="yegV"
                     /locus_tag="ACHOSC_RS11695"
                     /old_locus_tag="pgaptmp_002339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate kinase family protein"
                     /protein_id="HGU4548268.1"
                     /translation="MSGARLHTLLPELTSRQPVMVVGAAVIDVIADAYALPWRGCDIE
                     LKQQSVNVGGCALNIAVALKRLGIEAGNALPLGQGVWAEIIRNRMAKEGLISLIDNAE
                     GDNGWCLALVEPDGERTFMSFSGVENQWNRQWLARLTVAPGSLLYFSGYQLASPCGEL
                     LVEWLEKLQDVTPFIDFGPRIGDIPDALLARIMACRPLVSLNRQEAEIAAERFALSAE
                     ITTLGKQWQEKFAAPLIVRLDKEGAWYFSNDASGCIPAFPTQVVDTIGAGDSHAGGVL
                     AGLASGLPLADAVLLGNAVASWVVGHRGGDCAPTREELLLAHKNV"
     gene            complement(12274..13020)
                     /gene="yegW"
                     /locus_tag="ACHOSC_RS11700"
                     /old_locus_tag="pgaptmp_002340"
     CDS             complement(12274..13020)
                     /gene="yegW"
                     /locus_tag="ACHOSC_RS11700"
                     /old_locus_tag="pgaptmp_002340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HGU4548269.1"
                     /translation="MEQAHTQLIAQLNERILAADNTPLYIKFAETVKNAVRSGVLEHG
                     NILPGERDLSQLTGVSRITVRKAMQALEEEGVVTRSRGYGTQINNIFEYSLKEARGFS
                     QQVVLRGKKPDTLWVNKRVVKCPEEVAQQLAVEAGSDVFLLKRIRYVDEEAVSIEESW
                     VPAHLIHDVDAIGISLYDYFRSQHIYPQRTRSRVSARMPDAEFQSHIQLDSKIPVLVI
                     KQVALDQQQRPIEYSISHCRSDLYVFVCEE"
     gene            complement(13072..13890)
                     /gene="yegX"
                     /locus_tag="ACHOSC_RS11705"
                     /old_locus_tag="pgaptmp_002341"
     CDS             complement(13072..13890)
                     /gene="yegX"
                     /locus_tag="ACHOSC_RS11705"
                     /old_locus_tag="pgaptmp_002341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416605.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 25 protein"
                     /protein_id="HGU4548270.1"
                     /translation="MQLRITSRKKLTSLLCALGLISIVAIYPRQTVNFFYSTAVQITD
                     YIHFYGYRPVKSFAIRIPASYTIHGIDVSRWQERIDWQRVAKMRDNGIRLQFAFIKAT
                     EGEKLVDPYFSRNWQLSRENGLLRGAYHYFSPSVAAPVQARLFLQTVDFSQGDFPAVL
                     DVEERGKLSAKELRKRVSQWLKMVEKSTGKKPIIYSGAVFYHTNLAGYFNEYPWWVAH
                     YYQRRPDNDGMAWRFWQHSDRGQVDGINGPVDFNVFHGTVEELQAFVDGIKETP"
     gene            complement(13955..14755)
                     /gene="thiD"
                     /locus_tag="ACHOSC_RS11710"
                     /old_locus_tag="pgaptmp_002342"
     CDS             complement(13955..14755)
                     /gene="thiD"
                     /locus_tag="ACHOSC_RS11710"
                     /old_locus_tag="pgaptmp_002342"
                     /EC_number="2.7.1.49"
                     /EC_number="2.7.4.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008902 - hydroxymethylpyrimidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0008972 - phosphomethylpyrimidine kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional hydroxymethylpyrimidine
                     kinase/phosphomethylpyrimidine kinase"
                     /protein_id="HGU4548271.1"
                     /translation="MKRINALTIAGTDPSGGAGIQADLKTFSALGAYGCSVITALVAQ
                     NTRGVQSVYRIEPDFVAAQLDSVFSDVRIDTTKIGMLAETDIVEAVAERLQRYQIQNV
                     VLDTVMLAKSGDPLLSPSAVATLRSRLLPQVSLITPNLPEAAALLDAPHARTEQEMLE
                     QGRSLLAMGCGAVLMKGGHLDDEQSPDWLFTREGEQRFTAPRIMTKNTHGTGCTLSAA
                     LAALRPRHTNWADTVQEAKSWLSSALAQADTLEVGHGIGPVHHFHAWW"
     gene            complement(14752..15540)
                     /gene="thiM"
                     /locus_tag="ACHOSC_RS11715"
                     /old_locus_tag="pgaptmp_002343"
     CDS             complement(14752..15540)
                     /gene="thiM"
                     /locus_tag="ACHOSC_RS11715"
                     /old_locus_tag="pgaptmp_002343"
                     /EC_number="2.7.1.50"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004417 - hydroxyethylthiazole kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyethylthiazole kinase"
                     /protein_id="HGU4548272.1"
                     /translation="MQVDLLSSAQSAHALHLFHQHSPLVHCMTNDVVQTFTANTLLAL
                     GASPAMVIETEEASQFAAIASALLINVGTLTQLRAQSMCAAVEQAKSSQTPWTLDPVA
                     VGALDYRRRFCLELLSHKPTAIRGNASEIMALAGIANGGRGVDTTDAAANAIPAAQTL
                     ARETGAIVVVTGEMDYVTDGHRAVGIHGGDPLMTKVVGTGCALSAVVAACCALPGDTL
                     ENVASACHWMKQAGERAVARSEGPGSFVPHFLDALWQLTQEVQA"
     regulatory      complement(15575..15671)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            complement(15763..16035)
                     /gene="rcnR"
                     /locus_tag="ACHOSC_RS11720"
                     /old_locus_tag="pgaptmp_002344"
     CDS             complement(15763..16035)
                     /gene="rcnR"
                     /locus_tag="ACHOSC_RS11720"
                     /old_locus_tag="pgaptmp_002344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ni(II)/Co(II)-binding transcriptional repressor
                     RcnR"
                     /protein_id="HGU4548273.1"
                     /translation="MSHTIRDKQKLKARASKIQGQVVALKKMLDEPHECAAVLQQIAA
                     IRGAVNGLMREVIKGHLTEHIVHQGDELKREEDLDVVLKVLDSYIK"
BASE COUNT         3938 a         4186 c         3913 g         4046 t
ORIGIN      
        1 ttggttgaat attaggcata tgctcttttt tgaaatttat cggtggcagc cgttagtatt
       61 cgctgtcccc attgcaagct cctggtggta accactgaat cctccatact tgaactgact
      121 ttttatcctc cgactttcat cctgttctga ctccaccttt tgttttctgc tctacactat
      181 ctacagacca atcataaagg cacatacgat catggcagaa tttcccgcca gcttactgat
      241 tcttaatggc aaaagtactg acaatctacc cttgcgcgaa gcaattatgc tgttgcgtga
      301 ggaaggaatg acgatccatg tgcgggtcac ctgggagaaa ggcgatgccg cacgatatgt
      361 agaggaggcc cggaagttgg gcgtcgcaac ggtgattgcc ggtggtggcg atggcaccat
      421 taatgaagtt tctacggcgt tgattcagtg tgagggggat gacatacccg cgctgggaat
      481 tttgccatta ggaaccgcca atgattttgc caccagtgta gggattcctg aggcactgga
      541 taaggcgctg aaactggcaa ttgccggtaa cgccattgcg atagatatgg cgcaggtcaa
      601 caaacaaacc tgttttatta atatggcgac aggcggattt gggacgcgta ttaccacaga
      661 aacgccggaa aaattaaaag ccgcgctggg tggcgtctct tacatcattc atggcttaat
      721 gcgcatggat actctgcaac cggaccgttg tgaaatccgc ggtgaaaact ttcactggca
      781 aggtgacgcc ctggtcattg gtattggtaa cgggcgtcag gccggtggcg gtcagcaatt
      841 gtgtccgaac gcgttaatta acgatggctt gctgcaactg cgcattttta ccggcgatga
      901 aattcttccg gctctcgtat caaccttaaa atctgacgaa gataacccga atattatcga
      961 aggcgcttcg tcgtggtttg atatacaagc cccacacgaa atcactttta atcttgatgg
     1021 cgaaccgttg agcggacaaa actttcatat tgaaatactt ccggcggcgt tgcgttgtcg
     1081 attaccacca gattgtccat tattgcgtta atcaaactat tctctcttga accagggaga
     1141 atccgaaatg ccggatgcgg tgtaaatacc ttatccggca tacagaacaa tactacaccg
     1201 taattaattt tacccgtgtc ttctgcaatg ccagtttatc cacctctgat acagcatcat
     1261 cggtaataat aatgtcaatt ttttcgattg gcagtacctg attaaaaccg cgacggttaa
     1321 atttcgatga atcgagaacg gcaatcactt tattagcggc ggtgaccatc gcaccgctaa
     1381 tcgaataacc ttcattaaag gtcgtaatac cattaaccgc atcaatacca tcagcaccga
     1441 caaacattaa atcggcatta atatcctgca gggaacgttc agcaatagat ccatgcatag
     1501 atcgcgtttt atggcggacc gtgccaccgc agacgaccag agtaatgtct ttattttcgg
     1561 aaagggcaaa cgcagccggg agactgttgg taatcaccgt gatatttttg gcggtcatta
     1621 atccttcggc gataagcatt gtggtacttc cgctgtcgag aataacggtc atcccttcat
     1681 ggatcatttt gactgccgcc tgcgctattc tgtttttcgg cgcgctggcg agctgaaatc
     1741 gctctttaaa cccgacttcc tgggtctcgg tttcactatt accggacatt attttcgccg
     1801 caccgccatg aaagcgcgta accacgcctt tttgttcgag aaagcgcaaa tcggcacgga
     1861 ttgtcgcttc cgaggcagca aatactcccg ccagatcctg aacaagcacg gttccctgtt
     1921 cattcactaa ttggatgatc ttattccttc gctcgaatga attcatcgtc atttacccat
     1981 attcaattgt ggctagtgta aacgaagggg gaccattggt gaatgatctg ttttgcacaa
     2041 taccgggcca gcatcacgct ggcccgcggt ttcagggaat gagcaacact ttgcccggca
     2101 tagcattacg agcgatgtca cgcaccgcct gggcgaagct ttcaaagctt ccacggtgag
     2161 cgattaatgg ctccaggctt aacttacgtt ctgtcagcaa ccggctcgcc gtttcccact
     2221 cctgccccgg ccaagggctg gagtagttca tccaactgcc gataaccgtc agctctttac
     2281 gcaatatttt gccaaacgtt gccgatgtta aatgcagatc ctgatgcaac gtgcccacca
     2341 gcgccagttg ggcatgagga ccggcaatct ctaccgccag ttcgacagtt tgcggtacgc
     2401 cagccgtctc gaggataagc tgattaaagc gcagttagcg taaaacgctc tgcatttgcg
     2461 gcgcgctcat ttcgctactg ttaaatgttt gcatcgcacc gaaagatttt gccagtgcca
     2521 gtttttctga actaatgtcg atcgccgtca cactctttgc tcccagcgcg acagcgcact
     2581 gaatggccag caggccaatg gttccggcac caataataat aacgttttta ttctcacaac
     2641 cttgcgctaa atgaaaagca tgcaggccaa cggtaatcgg ttcaataaaa gccccatcct
     2701 caataggcat atcagtaggt agagcaaaga catttttccg cttaacgaca atatattcgg
     2761 caaatccacc atcacgccgt gagccaataa aatcatattt tgcgcactgg gaataaaacc
     2821 ctttcaaaca ctctggacaa gtaaaacagg gtaataacgg cacacaggca accgcatcgc
     2881 caggatgtaa atcatcaaca ccggatccca ccgcatcaat atagccgcta aattcatggc
     2941 ctaacgttat tggataataa tgtgcaccat ttttaaatat cctgggtaaa tcggaaccac
     3001 ataagcccga gctggcaatt tttacccgca cctcatcctg atgtttaatt tcaggaatga
     3061 cgctttctgc aacgcgcacg ataccatcag tatcattcac cactgatttc ataaaaactc
     3121 ctgattgtta agggggataa cctccccctc aggtaaaaat tattctgcga gaacgacttt
     3181 ctcttgttta ataaagccac gcgctctacg ccaggtcatg aaaataccgg taagataaat
     3241 tgcgccgata ataatgaatc cgggaatatt ttgcggggag aaaacctgaa tcagtaacca
     3301 ggtaatggga gaaccgccct gatccattga agccaccata cccccggctt ttaacgcccc
     3361 agcattagcc gccagttggg tgtgcaaacc aatagtttgc gtcgcgatcc acagagtgat
     3421 gctcatgatg atgacacccg agattaaggt gcggaacaga tttccacgat gcacggcgac
     3481 tgccatcgcc acaaagaagc cgatggtggc aagatcgcca aacggcagca cctgattacc
     3541 cggaacacaa acagcaatta aaatggtgag cgggataaaa atcaggcttg ccgataccac
     3601 cgccgtatgc cccagcagta atgcgggatc caggccaatc aggaactcct gaccgccgaa
     3661 cttcgcctgt aaacgactac gggcctgctt agcgatgggc gttaaaccat ccatgatggg
     3721 tttaatcacc cgtggcatta gcagcatcac tgccgccgtt tttaccgcca gctgcaatac
     3781 acctttgaca tcgtaacccg cgaggatgcc gataatcagc cccatcacaa aacccacggt
     3841 gacaggctcg ccaaatggac caaatttgcg ctgaatatcg tcggcgctaa atttaattcg
     3901 gttaacgcct gggattttct cgatgatagc atcgaccagc accgcaatcg gccccatata
     3961 cgccgacgta ccgtgcggaa tagcaatacc ttccagctca aagaaatttc gggtatcgcg
     4021 ggcaaaccag tcgccgagct tataaacaaa cgccgcgtga attaccacac ctgccatccc
     4081 tatcatccat gaaccggttg ccaggtgcag caacgcgccg gtgaaggtca tatgccagat
     4141 attccagata tcaacattta ccacccgcgt catacgggtc agtaacatcg ccacgttaac
     4201 caatatcgca atcggaatcg ccaccagcgc aatttgcgac gcccaggtca ttggtgaaga
     4261 gcccggccag ccgacatcga ccacatgcag attcaggtcg aaattttccg ccatcgcttt
     4321 cgccgccgga ccaatggaat ccagcattaa gccaatcaca aggccaatgc caacaaagcc
     4381 aatcccgata tgcagacccg ctttaaagca atcgcctgcc ttcatgccta atattttaga
     4441 aaaaataatg atgacaatcg gaagcatcac cgtagggccg aggtcgagaa tataacgcat
     4501 gacttctgaa aacataggtc acccctgtaa gatagtcaga attttatttt gtaatgcttc
     4561 gataccgaca ccagaaataa aaggcatgcc gtgaactaac ggaatatcgc caaaactacg
     4621 atccactttg gcagtggtgc atatcaaatg cacaccatcc atataggttt ctatttcatt
     4681 aacccgacac tggattaatt caacaggaat attatgattc tgacacaact ctttaatttc
     4741 ttccgccgcc atcgtagagg tcgcaaccgc gcctccgcaa gcgacaataa tcttgcgttt
     4801 cataaatagt cctttttatt gttacagagg gatttattcg tctgaatcta aaacatactt
     4861 tgtgaagtat tcctttaact gagtttcagg aagagtaatt agtgtctcga cgatatcggg
     4921 ctgttgtaac ttgccaaata aacagcgtaa aagtttcaat tgctgctgcg gattttccac
     4981 aatcaacgca ataaccaacg acacctccac gtcgttatca tcatccgctt gctgaaaatg
     5041 aactttattt gttggcctta acagataaat ggctgacgac ttagcatgaa tcgcctcaca
     5101 atgcggtata gcaatggcgt gctgctcaag cattatcccg gtagggaatt ctgcttctct
     5161 ggcaattaat gcctgtggcc aggtatcata aaccacacct ttcgcgagca tctcattacc
     5221 gatatgggtt aaaacttcgc tacgatcgac aaaagaaatt ccgctacgaa caaacaggtt
     5281 agtcatatac cgtccttatt ccgcacagcc gtagcgatag gcgcgcaaaa catcataaat
     5341 tttatccata atcagctgat gcggtattgc tgataattcc ccggactgaa tgcgttcaaa
     5401 ttgtttggga agatactgac taatcatgcc cagtgggatg tccacgcctt caagattcac
     5461 catcatcgtt tcgacgctat ttttaatccg actatgcggc cagtaataac gaatacgatc
     5521 cgacaggctg taacgaatat ccagtaatga atcgttaaaa cccgtacgat aatatttttt
     5581 ccagtattgc ggttcgtcga gcatcacttc ttcaattacc gccaggcaac cgctgcgatt
     5641 ttcaggggcg ataagttcct gctcaatttg tgccagtgca aatatcgcct cgcgtaaagc
     5701 aaaggttaat gcgggaccga ctttcaatat tgcaaagtga tcgcggacta attcccaata
     5761 agccgtccgg gtctggtaat cggtagaatg tgcttcataa accattcggg tgctttctat
     5821 ccattgcgcc agcggctgcg cttcctgcgg ctgataatgg ataatattgc tgtgatcaaa
     5881 ttccacgccc ggctgcacca cgatggcaat cacgcgtgtt aacgcctctg tcagcccacg
     5941 ggcaataaag gccttttgat gcgtacgtaa agtattggcg gcatcttcaa catgggtgat
     6001 gtgtactgac tgaatggcgc tggcctcacc gcccggaacc ggaacttcgg tgccaatgac
     6061 atagctcagt tgctcacgct ggcaatctgt cgccacactt tccgcagcaa agcaaagcac
     6121 agcagctcgt tccgcaaccg tttctggtgc taacggtatg ggatcccccg cgcaggacat
     6181 tgacgcatca agatgaattt tactgaaacc ggcacgaaca tattccttta ccagctcgac
     6241 ggatttttcc atcgccgcat ccgcattttc ttgctgccag cagtttggcc ccagatgatc
     6301 gccgccgaga ataatgcgtt cgcgtgcaaa cccaacttta tcggcaatcg taaaaacaaa
     6361 ttcgcgaaag tctgccggtg tcattccggt ataaccgcca aattgattga cctggtttga
     6421 cgttgcttca atcagcactt tgcgcgtgct gttgcgatca aatgccagcg ccgcttcgat
     6481 aaccaacgga tgggcagaac agactgaaca tatgccgata tgttcaccag ctttatgccg
     6541 ggcaattaac gttttcatgt tttttcctta ttaaatggca ggtgcgttat gccctgccct
     6601 cgcagccaca atcggcaatc actttgctca ccacatcgcg cattgcggat ttagccgact
     6661 gcaaataatc ccggggatcg gtcgcttcag ggtgctcggt caggtaattt tttaacgcct
     6721 gcgagaaggc atttttcagc tccgttgcaa cgttgatttt gcatatcccc agtttgatgg
     6781 tttgctgaat atccttagtc gataaccctg acgcgccatg cagcaccagc ggtaagttca
     6841 cccactggcg aatgttctcc agtcgagaaa aatcaagcgc cggtgcgctg gcatacatcc
     6901 catgagccgt gccgatggcg accgccaggg aatcaattcc ggttgcctcg gcaaattcac
     6961 gcgcctgagc ggggttggta taaaaagcat cggcttcatt gacttgcaca tcatcttcct
     7021 ggccgccaag ttgccccagc tccgcttcga cgctgacatc aaagcgatgg caaaaatcca
     7081 ccacctcttt gacccgtgaa atattttgcg caaaaggcaa atgcgaggcg tcaatcatga
     7141 ctgagcgcac gccagaacga accttctgag cgatatcgtc aaatttcgtg tgatggtcga
     7201 gatgaattgc cagtggatgg tgatattgct tcgccatcgc gctgaccagc gccaacagat
     7261 tttctgtacc agcatgagta aatgtgccag gcgttccggc gatgatgacc ggcgcatgca
     7321 ggttggcagc ggtttccacc accacttgca tcgtttcgag attgtgaata ttgaatgccg
     7381 gaaccgcata accgccgcgc tgtgcgttgt tcagcatctg ctttgtcgat accacgtaca
     7441 ttttcatatc ctgtcgtttg ttttcgattt caaaatataa tgaaatgatt tgtttttaaa
     7501 tgtcgagata acgatcacaa aagcgacaat gtgaaaattg ttcgaggagt aaaaggcaaa
     7561 aaaaaacggc ctcccgatag ggaagccgta gcaaagtgcg cgtgttttta tgccggatgc
     7621 ggtgtaaaca tacgtggcag tgctatgtag gcatgataag acgcttccgc gtcgcatcag
     7681 gcataggttg ccggatgcag tgtaaacgcc ttatccgtcc tacggggtgg tgctgttcgt
     7741 atgaggcgta tcaggcaata gtaattttgc tatcgagata aacgtcctgc acggcattaa
     7801 tcagtttcac gccgtcagcc atcgatttct tgaacgcctt acgtccaaga atcagcccca
     7861 ttccgccagc gcgtttgttg ataaccgcag tacgcactgc atcgctgagg tcagtttcac
     7921 cgcccgcagc accgccggag tttatcaacc cagcccgacc catatagcag ttagctaact
     7981 gataacgcac cagatcaatc gggttttcgc tggtcaattt gctgtaaaca cgatcgtcgg
     8041 tgtaaccgta attaattgct ttatagccgc cgttattttc cgccattttt tgtttgacga
     8101 tatctgcacc gatggttgcc gccagatggt ttgcctgacc ggtcaggtcg gcggaaacat
     8161 ggtaatcaac gccatctttc ttaaaggcgg agttacgcaa ataggcccac agcactgtca
     8221 ccatacccag ctcgtgcgca cgttcaaaag ccgcagaaat ttcttcaatc tggcgacgtg
     8281 actcttccga gccaaaatag atagtcgcac caaccgcaac cgcgcccatg ttgaacgcct
     8341 gctccacgct ggcatacagc gtttgatcgt aggtattcgg gtaacttagc gtctcgttgt
     8401 gattaagttt gacgaggaat ggaatgcgat gcgcataacg ccgcgatacc gacgccagca
     8461 cgccgtaagt tgacgccaca cagttacagc ccgcttcgat cgccagttca acaatgtttt
     8521 tcgggtcaaa gtagagcggg ttagcagcaa atgaagctcc ggcagagtgc tcaacgccct
     8581 ggtcaaccgg cagaatagaa agatatcctg tgccagccag acgcccggtg ttgtacaacg
     8641 tctgcatatt acgtaacacc gctggcgggc gattattgtc aatcattacg cggtctacgt
     8701 agtcatgtcc ggggagataa agctggtcag aaggaattgt catacaacgg tgctgtaaaa
     8761 ggttgtcggc gtctttgcca agcaactgcg caatatctgt cattactatg ctcccgtaaa
     8821 ttccgattgg atatcggcta tggattgtcc tggcccgcct tttgcgggca atcataatcc
     8881 tggtcgttac gggcgagttt ttccatcttt tggatgtttt ttcagcgttt tctgttggct
     8941 cgattcatca gaaaaaatgt tagcgcggtc aagttttcac cgcaaaggta tttaaaaggt
     9001 attattaagt ggtattgtca tcgcgtacct tacattacct gtcatgaagg aattaaaaga
     9061 tgaaaacaac agcaaagctg tcgttcatga tgtttgttga atggtttatc tggggcgcgt
     9121 ggtttgtgcc attgtggttg tggttaagta aaagcggttt tagtgccgga gaaattggct
     9181 ggtcgtatgc ctgcacagcc attgcggcga tcctgtcgcc aattctggtt ggctccatca
     9241 ctgaccgctt tttctcggcg caaaaagtgc tggcggtatt gatgttcgca ggcgcgctgc
     9301 tgatgtattt cgctgcgcaa cagaccactt ttgccgggtt cttcccgtta ctgctggcct
     9361 actcgctaac ctatatgccg accattgcgc tgactaacag tatcgctttt gccaacgtgc
     9421 cggatgtgga gcgtgatttc ccgcgcattc gtgtgatggg cactatcggc tggattgcct
     9481 ctggtctggc atgtggtttc ttgccgcaaa tgctggggta tgccgatatc tcaccgacta
     9541 acatcccgct gctgattacc gccggaagtt ctgctctgct cggtgtgttt gcgtttttcc
     9601 tgcccgacac gccgccaaaa agcaccggca aaatggacat taaagtcatg ctcggcctgg
     9661 atgcgctgat cctgctgcgc gataaaaact tcctcgtctt tttcttctgt tcattcctgt
     9721 ttgcgatgcc actggcgttc tattacatct ttgccaacgg ttatctgacc gaagttggca
     9781 tgaaaaacgc caccggctgg atgacgctcg gccagttctc tgaaatcttc tttatgctgg
     9841 cattgccgtt tttcactaaa cgctttggta tcaaaaaggt attattgctt ggtctggtca
     9901 ccgctgcgat ccgctatggc ttctttattt acggtagtgc ggatgaatat ttcacctacg
     9961 cgttactgtt cctcggtatt ttgcttcacg gcgtaagtta cgatttttac tacgttaccg
    10021 cttacatcta tgtcgataaa aaagcccccg tgcatatgcg taccgccgcg caggggctga
    10081 tcacgctctg ctgccagggc ttcggcagtt tgctcggcta tcgtcttggc ggtgtgatga
    10141 tggaaaagat gttcgcttat ccggaaccgg taaacgggct gactttcaac tggtccggga
    10201 tgtggacttt cggcgcggtg atgattgcca ttatcgccgt gctgttcatg atttttttcc
    10261 gcgaatccga caacgaaatt acggctatca aggtcgatga tcgcgatatt gcgttgacac
    10321 aaggggaagt taaatgaaaa cagaacgtat tctcggtgct ctttatgggc aggcgttagg
    10381 ggatgcgatg gggatgccct ccgagctttg gccacgcagc cgcgttaaag cacactttgg
    10441 ctggattgac cgttttctgc ctggaccaaa ggagaataac gcggcctgtt attttaaccg
    10501 cgccgaattc accgacgata cctcgatggc gctgtgtctg gcggatgcgt tactggaacg
    10561 tgaaggcaag atcgatccgg atctgattgg gcgtaatatt ctcgactggg cgctgcgttt
    10621 cgacgccttt aacaaaaacg tactaggtcc gacctcgaag attgcgctta acgccattcg
    10681 cgacggtaaa cccgttgctg aactggaaaa caacggcgtg accaacggcg cagcgatgcg
    10741 cgtctcgcca ttaggttgtt tgcttccggc gcgtgatgtt gattccttta ttgatgatgt
    10801 ggcgctggcg tcgagcccga cccataaatc cgatctggca gttgcaggtg cggtagtcat
    10861 cgcatgggcg atttctcgtg ccattgacgg agaaagctgg tcagcgattg ttgattccct
    10921 gccttcaatt gcgcgacatg cacagcaaaa acgcatcact accttcagcg cctcactggc
    10981 agcacgtctg gagattgcgc tgaaaattgt gcgcaatgcc gacggcaccg aatccgccag
    11041 cgaacagctt taccaggtcg ttggcgcagg taccagcact attgagtccg ttccgtgcgc
    11101 cattgcgctg gttgaactgg cacaaaccga cccgaatcgc tgcgccgtcc tgtgcgctaa
    11161 ccttggcggc gacacagaca ccatcggcgc tatggcgacg gcgatttgcg gcgcgttgca
    11221 tggcgttaac gctatcgatc ctgcattaaa ggcggaactg gatgcggtaa atcagcttga
    11281 tttcaaccgc tatgccacag cgctggcgaa atatcgtcaa caacgggagg cggtatgagc
    11341 ggcgctcgat tacacacgct gctgcctgaa ttaacctcgc gtcagccggt gatggtcgtc
    11401 ggcgcggcgg tcattgatgt gatcgccgac gcttatgccc tcccctggcg tgggtgcgat
    11461 atcgaactga agcagcagag cgttaacgtt ggcggctgcg cactgaatat tgccgtggcg
    11521 ttaaagcgcc tcggcatcga agcgggtaat gccttgccgc tcggtcaggg cgtgtgggcg
    11581 gagattattc gcaaccggat ggcgaaagag gggttaatca gcctgatcga taacgccgaa
    11641 ggcgataacg gctggtgtct ggcgctggtt gagccggatg gcgaacgcac ttttatgtca
    11701 ttcagcggtg tggaaaatca gtggaatcgc cagtggctgg cgcgattaac cgttgcgcct
    11761 ggcagcctgc tctatttttc cggttatcaa ctggcctcgc cctgcggcga attgttagtg
    11821 gaatggctgg aaaagctgca agacgtgacg ccgtttatcg attttggccc acgtatcggc
    11881 gatatcccgg acgcattact ggcgcggatc atggcctgtc gacctttagt atcactcaat
    11941 cgtcaagagg ctgagattgc cgccgaacgt tttgctttat ccgcagagat aacaacactt
    12001 ggcaagcaat ggcaggagaa atttgccgcg ccgttgatcg ttcgtctcga taaagagggt
    12061 gcatggtatt tcagcaacga cgcttctggc tgcattccgg catttccaac gcaagttgta
    12121 gacaccattg gggcgggcga cagtcatgca ggtggcgtac ttgccgggct ggcctctggt
    12181 ctgccactgg cggatgccgt attactgggc aatgcagtgg cgtcgtgggt tgtcgggcat
    12241 cggggcggtg attgtgcgcc aacgcgcgag gaactactcc tcgcacacaa aaacgtatag
    12301 atcgctgcga cagtggctaa tgctgtactc aataggccgt tgctgttggt caagcgccac
    12361 ttgcttgatc accagcaccg gtattttgct atctaactga atatgtgact gaaactcggc
    12421 atccggcatc cgggcgctaa cgcgggaacg cgtacgttgt gggtaaatgt gctggctgcg
    12481 gaaatagtca taaagtgaaa tcccgatggc atcaacatca tgaattaaat gcgccggaac
    12541 ccacgattcc tcaatcgata ccgcttcttc atcgacatag cgaatacgct taagcaaaaa
    12601 gacatcactt cctgcttcga ccgccagctg ctgcgcgact tcttcgggac atttcacgac
    12661 acgcttgtta acccatagcg tatcgggctt tttcccgcgc aataccacct gctgagaaaa
    12721 acctcgcgct tctttcaacg agtattcgaa gatgttgttg atctgcgtgc cgtaaccgcg
    12781 cgaacgcgtc accacaccct cttcttccag cgcctgcatc gccttgcgca ccgtaatgcg
    12841 cgaaacgccg gttaactgac ttaaatcacg ctcaccgggc aaaatattgc catgctccag
    12901 caccccgctg cgcacggcat tttttaccgt ttcggcaaac ttaatataaa gcggcgtgtt
    12961 atccgccgct aaaatacgct cgttcagttg ggcaatcagc tgggtatgcg cttgttccat
    13021 ttgtcttttt cctgccgggg agtctggtgg gagtatatca ctgcttctta tttaaggcgt
    13081 ttctttaatc ccatcaacga atgcctgcaa ctcctccacc gtgccatgaa atacattaaa
    13141 atccaccgga ccattgatgc catctacctg tccacggtcg gaatgctgcc agaagcgcca
    13201 ggccatgccg tcattgtccg gacgacgttg ataatagtga gccacccacc acggatattc
    13261 attgaaatag cccgccagat tggtgtgata aaaaacggct cctgagtaaa taatcggctt
    13321 ttttcccgta cttttttcga ccatttttag ccactgactt actcgtttgc gtaattcttt
    13381 tgccgataat tttccccgtt cttctacgtc cagtaccgca gggaaatcgc cttgtgagaa
    13441 atccaccgtt tgcagaaata atcgtgcctg aaccggcgct gctaccgacg gggagaaata
    13501 atgatacgcc ccgcgcagca ggccattttc gcggcttagt tgccagttac gcgaaaaata
    13561 cgggtccacc agcttttcgc cttccgtcgc cttaataaaa gcaaactgta agcggatacc
    13621 gttgtcgcgc atttttgcca cccgctgcca gtcgatccgc tcctgccagc gagaaacatc
    13681 tattccgtga atggtgtaac tggcaggaat gcgaatagcg aaagatttaa ccgggcgata
    13741 accgtagaaa tggatgtagt ctgtaatctg aactgctgtc gaatagaaaa aattcaccgt
    13801 ctgacgcgga taaattgcaa cgatggaaat tagcccaaga gcgcataata gagaggtgag
    13861 tttttttcga ctggtaattc ttagctgcat tggtttcatc cctgaatgtc agtgccaaag
    13921 gctgacaata accaaagcag ctatagtacg gtgctcacca ccaggcgtgg aagtggtgaa
    13981 ccggaccaat accgtgacca acttccagcg tgtcggcctg ggctaatgct gatgaaagcc
    14041 agctttttgc ctcctgtacg gtgtcagccc agtttgtatg gcgcgggcgt agtgcagcca
    14101 acgccgcaga gagcgtacaa ccagtgccgt gggtgttttt ggtcataatg cgcggtgcgg
    14161 taaaccgttg ttcaccctcg cgggtaaaca gccagtccgg gctttgctca tcatccagat
    14221 gaccaccttt cattagcact gcgccacagc ccatcgccaa cagcgatcgc ccttgttcca
    14281 gcatttcctg ttcggtgcgc gcgtgtggcg cgtcgagcaa ggcggcagct tcgggcaagt
    14341 ttggcgttat taatgaaacc tgtggcaata atcgactgcg cagcgtagca accgccgaag
    14401 gtgaaagcag cgggtcgccg ctttttgcca gcataacggt gtcgagtacc acgttttgga
    14461 tctgataacg ttgcaaccgt tctgccaccg cttcaacaat atcggtttcc gccaacatac
    14521 cgattttagt ggtgtcgatt cgcacatcgc tgaacaccga atcgagctgg gcggcgacaa
    14581 aatcaggctc aatgcgatac accgactgta cgccacgggt attttgcgcc accagtgcag
    14641 taataactga gcaaccataa gcgccaagtg ccgagaaggt tttaagatcg gcctgaatcc
    14701 ccgcaccacc actgggatca gtaccggcaa tcgtcagagc gttaattcgt ttcatgcctg
    14761 cacctcctgc gtcaattgcc agagcgcatc aaggaaatgt ggaacaaaac tgcctggccc
    14821 ctcgcttctg gcgactgcgc gctctccggc ttgtttcatc cagtgacagg cagatgcgac
    14881 attttccagc gtatcgcctg gtaacgcaca gcaggcagcg acaaccgccg ataatgcaca
    14941 gccagttcct accactttgg tcattaacgg atcgccaccg tgaatgccaa cggcgcgatg
    15001 gccatcggta acataatcca tctcgccagt gaccacgacg attgcgccag tttcccgtgc
    15061 cagtgtttgt gcagcgggta tcgcgttagc tgcggcgtca gtggtatcca ctccccgtcc
    15121 accattagca atgccagcta atgccatgat ttccgaagca ttaccacgta tggcggttgg
    15181 cttatgagac agaagttcca gacaaaaacg gcggcgataa tcgagtgcac caaccgctac
    15241 tggatcaagc gtccagggtg tttgagagct ttttgcttgc tcaacggcgg cacacatcga
    15301 ctgtgcgcgt agctgcgtca gcgtgccaac gttaatcagc aacgcactgg caatagccgc
    15361 aaactgactg gcctcttcgg tttcgataac catcgctggc gatgcaccga gcgccagcaa
    15421 ggtattggca gtaaaggttt gcaccacatc gttggtcatg cagtgcacaa gaggggaatg
    15481 ttggtgaaaa aggtgtaacg cgtgcgcaga ttgcgctgaa ctcagcaggt cgacttgcat
    15541 agtttgctcc tgccataacg tgaagaagca atgacctggt ggtccgtgac ttccctacgc
    15601 tggcattatc cagatcaggt gatacgggta tttctcagcc ttcacgcaga agggcacccc
    15661 gagtcgtttg gttgcgatga taaatcgcag aggaggatgg taatgtccag cgcacgcgtt
    15721 gtaaacgagg aaaaacggca gttttacaat cgcgttaata aattatttga tatatgaatc
    15781 cagcaccttc agaacgacat ccagatcttc ttcacgtttt agctcatccc cctggtgaac
    15841 gatgtgttcc gtcagatgac ctttaatcac ttcccgcatc agaccgttta ccgcgccacg
    15901 gatagcagca atctgttgta aaactgcagc gcattcgtgc ggttcgtcga gcattttctt
    15961 gagcgccacg acctggccct gaatcttact ggcacgcgct ttcagtttct gtttatcacg
    16021 gattgtatga gacatggcaa cacctggtta acaagaatat gaaaaatcat agcactatta
    16081 atc
//
LOCUS       DAZKHF020000088        16058 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2201S31241
            SAMN43267800-rid25106413.denovo.089, whole genome shotgun sequence.
ACCESSION   DAZKHF020000088 DAZKHF020000000
VERSION     DAZKHF020000088.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267800
            Sequence Read Archive: SRR30307263
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 16058)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16058)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 16058)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350581.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350581.2
            Genome Coverage       :: 151x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:21:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,652
            CDSs (total)                      :: 5,557
            Genes (coding)                    :: 5,320
            CDSs (with protein)               :: 5,320
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 237
            CDSs (without protein)            :: 237
            Pseudo Genes (ambiguous residues) :: 0 of 237
            Pseudo Genes (frameshifted)       :: 102 of 237
            Pseudo Genes (incomplete)         :: 130 of 237
            Pseudo Genes (internal stop)      :: 52 of 237
            Pseudo Genes (multiple problems)  :: 41 of 237
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16058
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267800-rid25106413.denovo.089"
                     /serotype="O 26:H11"
                     /isolate="2201S31241"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-01"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            complement(<1..113)
                     /gene="yojO"
                     /locus_tag="ACHOSC_RS11725"
                     /old_locus_tag="pgaptmp_002345"
     CDS             complement(<1..113)
                     /gene="yojO"
                     /locus_tag="ACHOSC_RS11725"
                     /old_locus_tag="pgaptmp_002345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YojO"
                     /protein_id="HGU4548274.1"
                     /translation="MHTPIGVKPVAGSKEWREAWQKRAFAHISNGYKHIYIA"
     gene            222..716
                     /gene="napF"
                     /locus_tag="ACHOSC_RS11730"
                     /old_locus_tag="pgaptmp_002346"
     CDS             222..716
                     /gene="napF"
                     /locus_tag="ACHOSC_RS11730"
                     /old_locus_tag="pgaptmp_002346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin-type protein NapF"
                     /protein_id="HGU4548275.1"
                     /translation="MKIDASRRGILTGRWRKASNGIRPPWSGDESHFLTHCTRCDACI
                     NACENNILQRGAGGYPSVNFKNNECSFCYACAQACPESLFSPRHTRAWDLQFTIGDAC
                     LAYQSVECRRCQDSCEPMAIIFRPTLSGIYQPQLNSQLCNGCGACAASCPVSAITAEY
                     LHAH"
     gene            706..969
                     /gene="napD"
                     /locus_tag="ACHOSC_RS11735"
                     /old_locus_tag="pgaptmp_002347"
     CDS             706..969
                     /gene="napD"
                     /locus_tag="ACHOSC_RS11735"
                     /old_locus_tag="pgaptmp_002347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chaperone NapD"
                     /protein_id="HGU4548276.1"
                     /translation="MHTNWQVCSLVVQAKSERISDISTQLNAFPGCEVAVSDAPSGQL
                     IVVVEAEDSETLIQTIESVRNVEGVLAVSLVYHQQEEQGEETP"
     gene            966..3452
                     /gene="napA"
                     /locus_tag="ACHOSC_RS11740"
                     /old_locus_tag="pgaptmp_002348"
     CDS             966..3452
                     /gene="napA"
                     /locus_tag="ACHOSC_RS11740"
                     /old_locus_tag="pgaptmp_002348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008940 - nitrate reductase activity
                     [Evidence IEA];
                     GO_function: GO:0030151 - molybdenum ion binding [Evidence
                     IEA];
                     GO_function: GO:0043546 - molybdopterin cofactor binding
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase catalytic subunit NapA"
                     /protein_id="HGU4548277.1"
                     /translation="MKLSRRSFMKANAVAAAAAAAGLSVPGVARAVVGQQEAIKWDKA
                     PCRFCGTGCGVLVGTQQGRVVACQGDPDAPVNRGLNCIKGYFLPKIMYGKDRLTQPLL
                     RMKNGKYDKEGEFTPITWDQAFDVMEEKFKTALKEKGPESIGMFGSGQWTIWEGYAAS
                     KLFKAGFRSNNIDPNARHCMASAVVGFMRTFGMDEPMGCYDDIEQADAFVLWGANMAE
                     MHPILWSRITNRRLSNQNVTVAVLSTYQHRSFELADNGIIFTPQSDLVILNYIANYII
                     QNNAINQDFFSKHVNLRKGATDIGYGLRPTHPLEKAAKNPGSDASEPMSFEDYKAFVA
                     EYTLEKTAEMTGVPKDQLEQLAQLYADPNKKVISYWTMGFNQHTRGVWANNLVYNLHL
                     LTGKISQPGCGPFSLTGQPSACGTAREVGTFAHRLPADMVVTNEKHRDICEKKWNIPS
                     GTIPAKIGLHAVAQDRALKDGKLNVYWTMCTNNMQAGPNINEERMPGWRDPRNFIIVS
                     DPYPTVSALAADLILPTAMWVEKEGAYGNAERRTQFWRQQVQAPGEAKSDLWQLVQFS
                     RRFKTEEVWPEELLAKKPELRGKTLYEVLYATPEVSKYPVSELAEDQLNDESRELGFY
                     LQKGLFEEYAWFGRGHGHDLAPFDDYHKARGLRWPVVNGKETQWRYSEGNDPYVKAGE
                     GYKFYGKPDGKAVIFALPFEPAAEAPDEEYDLWLSTGRVLEHWHTGSMTRRVPELHRA
                     FPEAVLFIHPLDAKARDLRRGDKVKVVSRRGEVISIVETRGRNRPPQGLVYMPFFDAA
                     QLVNKLTLDATDPLSKETDFKKCAVKLEKV"
     gene            3459..4154
                     /gene="napG"
                     /locus_tag="ACHOSC_RS11745"
                     /old_locus_tag="pgaptmp_002349"
     CDS             3459..4154
                     /gene="napG"
                     /locus_tag="ACHOSC_RS11745"
                     /old_locus_tag="pgaptmp_002349"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin-type protein NapG"
                     /protein_id="HGU4548278.1"
                     /translation="MSRSAKPQNGRRRFLRDVVRTAGGLAAVGVALGLQQQTARASGV
                     RLRPPGAINENAFASACVRCGQCVQACPYDTLKLATLASGLSAGTPYFVARDIPCEMC
                     EDIPCAKVCPSGALDREIESIDDARMGLAVLVDQENCLNFQGLRCDVCYRECPKIDEA
                     ITLELERNTRTGKHARFLPTVHSDACTGCGKCEKVCVLEQPAIKVLPLSLAKGELGHH
                     YRFGWLEGNNGKS"
     gene            4141..5004
                     /gene="napH"
                     /locus_tag="ACHOSC_RS11750"
                     /old_locus_tag="pgaptmp_002350"
     CDS             4141..5004
                     /gene="napH"
                     /locus_tag="ACHOSC_RS11750"
                     /old_locus_tag="pgaptmp_002350"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinol dehydrogenase ferredoxin subunit NapH"
                     /protein_id="HGU4548279.1"
                     /translation="MANRKRDAGREALEKKGWWRSHRWLVLRRLCQFFVLGMFLSGPW
                     FGVWILHGNYSSSLLFDTVPLTDPLMTLQSLASGHLPATVALTGAVIITVLYALAGKR
                     LFCSWVCPLNPITDLANWLRRRFDLNQSATIPRHIRYVLLVVILLGSALTGTLIWEWI
                     NPVSLMGRSLVMGFGSGALLILALFLFDLLVVEHGWCGYICPVGALYGVLGSKGVITV
                     AATDRQKCNRCMDCFHVCPEPHVLRAPVLDEQSPVQVTSRDCMTCGRCVDVCSEDVFT
                     ITTRWSSGAKS"
     gene            5001..5450
                     /gene="napB"
                     /locus_tag="ACHOSC_RS11755"
                     /old_locus_tag="pgaptmp_002351"
     CDS             5001..5450
                     /gene="napB"
                     /locus_tag="ACHOSC_RS11755"
                     /old_locus_tag="pgaptmp_002351"
                     /EC_number="1.9.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311119.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase cytochrome c-type subunit"
                     /protein_id="HGU4548280.1"
                     /translation="MKSHDLKKALCQWTAMLALVVSGAVWAANGVDFSQSPEVSGTQE
                     GAIRMPKEQERMPLNYVNQPPMIPHSVEGYQVTTNTNRCLQCHGVESYRTTGAPRISP
                     THFMDSDGKVGAEVAPRRYFCLQCHVPQADTAPIVGNTFTPSKGYGK"
     gene            5460..6062
                     /gene="napC"
                     /locus_tag="ACHOSC_RS11760"
                     /old_locus_tag="pgaptmp_002352"
     CDS             5460..6062
                     /gene="napC"
                     /locus_tag="ACHOSC_RS11760"
                     /old_locus_tag="pgaptmp_002352"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c-type protein NapC"
                     /protein_id="HGU4548281.1"
                     /translation="MGNSDRKPGLIKRLWKWWRTPSRLALGTLLLIGFVGGIVFWGGF
                     NTGMEKANTEEFCISCHEMRNTVYQEYMDSVHYNNRSGVRATCPDCHVPHEFVPKMIR
                     KLKASKELYGKIFGVIDTPQKFEAHRLTMAQNEWRRMKDNNSQECRNCHNFEYMDTTA
                     QKSVAAKMHDQAVKDGQTCIDCHKGIAHKLPDMREVEPGF"
     gene            6075..6698
                     /gene="ccmA"
                     /locus_tag="ACHOSC_RS11765"
                     /old_locus_tag="pgaptmp_002353"
     CDS             6075..6698
                     /gene="ccmA"
                     /locus_tag="ACHOSC_RS11765"
                     /old_locus_tag="pgaptmp_002353"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416705.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c biogenesis heme-transporting ATPase
                     CcmA"
                     /protein_id="HGU4548282.1"
                     /translation="MGMLEARELLCERDERTLFSGLSFTLNAGEWVQITGSNGAGKTT
                     LLRLLTGLSRPDAGEVLWQGQPLHQVRDSYHQNLLWIGHQPGIKTRLTALENLHFYHR
                     DGDTAQCLEALAQAGLAGFEDIPVNQLSAGQQRRVALARLWLTRATLWILDEPFTAID
                     VNGVDRLTQRMAQHTEQGGIVILTTHQPLNVAESKIRRISLTQTRAA"
     gene            6695..7357
                     /gene="ccmB"
                     /locus_tag="ACHOSC_RS11770"
                     /old_locus_tag="pgaptmp_002354"
     CDS             6695..7357
                     /gene="ccmB"
                     /locus_tag="ACHOSC_RS11770"
                     /old_locus_tag="pgaptmp_002354"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015232 - heme transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015886 - heme transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heme exporter protein CcmB"
                     /protein_id="HGU4548283.1"
                     /translation="MMFWRIFRLELRVAFRHSAEIANPLWFFLIVITLFPLSIGPEPQ
                     LLARIAPGIIWVAALLSSLLALERLFRDDLQDGSLEQLMLLPLPLPAVVLAKVMAHWM
                     VSGLPLLILSPLVAMLLGMDVYGWQVMALTLLLGTPTLGFLGAPGVALTVGLKRGGVL
                     LSILVLPLTIPLLIFATAAMDAASMHLPVDGYLAILGALLAGTATLSPFATAAALRIS
                     IQ"
     gene            7399..8136
                     /gene="ccmC"
                     /locus_tag="ACHOSC_RS11775"
                     /old_locus_tag="pgaptmp_002355"
     CDS             7399..8136
                     /gene="ccmC"
                     /locus_tag="ACHOSC_RS11775"
                     /old_locus_tag="pgaptmp_002355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme exporter protein CcmC"
                     /protein_id="HGU4548284.1"
                     /translation="MWKTLHQLAIPPRLYQICGWFIPWLAIASVVVLTVGWIWGFGFA
                     PADYQQGNSYRIIYLHVPAAIWSMGIYASMAVAAFIGLVWQMKMANLAVAAMAPIGAV
                     FTFIALVTGSAWGKPMWGTWWVWDARLTSELVLLFLYVGVIALWHAFDDRRLAGRAAG
                     ILVLIGVVNLPIIHYSVEWWNTLHQGSTRMQQSIDPAMRSPLRWSIFGFLLLSATLTL
                     MRMRNLILLMEKRRPWVSELILKRGRK"
     gene            8133..8342
                     /gene="ccmD"
                     /locus_tag="ACHOSC_RS11780"
                     /old_locus_tag="pgaptmp_002356"
     CDS             8133..8342
                     /gene="ccmD"
                     /locus_tag="ACHOSC_RS11780"
                     /old_locus_tag="pgaptmp_002356"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0015886 - heme transport [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heme exporter protein CcmD"
                     /protein_id="HGU4548285.1"
                     /translation="MTPAFASWNEFFAMGGYAFFVWLAVVMTVIPLVVLVVHSVMQHR
                     AILRGVAQQRAREARLRAAQQQEAA"
     gene            8339..8818
                     /gene="ccmE"
                     /locus_tag="ACHOSC_RS11785"
                     /old_locus_tag="pgaptmp_002357"
     CDS             8339..8818
                     /gene="ccmE"
                     /locus_tag="ACHOSC_RS11785"
                     /old_locus_tag="pgaptmp_002357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c maturation protein CcmE"
                     /protein_id="HGU4548286.1"
                     /translation="MNIRRKNRLWIACAVLAGLALTIGLVLYALRSNIDLFYTPGEIL
                     YGKRETQQMPEVGQRLRVGGMVMPGSVQRDPNSLKVTFTIYDAEGSVDVSYEGILPDL
                     FREGQGVVVQGELEKGNHILAKEVLAKHDENYTPPEVEKAMEANHRRPASVYKDPAS"
     gene            8815..10758
                     /gene="ccmF"
                     /locus_tag="ACHOSC_RS11790"
                     /old_locus_tag="pgaptmp_002358"
     CDS             8815..10758
                     /gene="ccmF"
                     /locus_tag="ACHOSC_RS11790"
                     /old_locus_tag="pgaptmp_002358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c-type biogenesis heme lyase CcmF"
                     /protein_id="HGU4548287.1"
                     /translation="MMPEIGNGLLCLALGIALLLSVYPLWGVARGDARMMASSRLFAW
                     LLFMSVAGAFLVLVNAFVVNDFTVTYVASNSNTQLPVWYRVAATWGAHEGSLLLWVLL
                     MSGWTFAVAIFSQRIPLDIVARVLAIMGMVSVGFLLFILFTSNPFSRTLPNFPIEGRD
                     LNPLLQDPGLIFHPPLLYMGYVGFSVAFAFAIASLLCGRLDSTYARFTRPWTLAAWIF
                     LTLGIVLGSAWAYYELGWGGWWFWDPVENASFMPWLVGTALMHSLAVTEQRASFKAWT
                     LLLAISAFSLCLLGTFLVRSGVLVSVHAFASDPARGMFILAFMVLVIGGSLLLFAARG
                     HKVRSRVNNALWSRESLLLANNVLLVAAMLVVLLGTLLPLVHKQLGLGSISIGEPFFN
                     TMFTWLMVPFALLLGVGPLVRWGRDRPRKIRNLLIIAFISTLVLSLLLPWLFESKVVA
                     MTVLGLAMACWIAVLAIAEAALRISRGTKTTFSYWGMVAAHLGLAVTIVGIAFSQNYS
                     VERDVRMKSGDSVDIHEYRFTFRDVKEVTGPNWRGGVATIGVTRDGKPETVLYAEKRY
                     YNTAGSMMTEAAIDGGITRDLYAALGEELENGAWAVRLYYKPFVRWIWAGGLMMALGG
                     LLCLFDPRYRKRVSPQKTAPEAV"
     gene            10755..11312
                     /gene="dsbE"
                     /locus_tag="ACHOSC_RS11795"
                     /old_locus_tag="pgaptmp_002359"
     CDS             10755..11312
                     /gene="dsbE"
                     /locus_tag="ACHOSC_RS11795"
                     /old_locus_tag="pgaptmp_002359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiol:disulfide interchange protein DsbE"
                     /protein_id="HGU4548288.1"
                     /translation="MKRKVLLIPLIIFLAIAAALLWQLARNAEGDDPTNLESALIGKP
                     VPKFRLESLDNPGQFYQADVLTQGKPVLLNVWATWCPTCRAEHQYLNQLSAQGIRVVG
                     MNYKDDRQKAISWLKELGNPYALSLFDGDGMLGLDLGVYGAPETFLIDGNGIIRYRHA
                     GDLNPRVWEEEIKPLWEKYSKEAAQ"
     gene            11309..12361
                     /gene="ccmH"
                     /locus_tag="ACHOSC_RS11800"
                     /old_locus_tag="pgaptmp_002360"
     CDS             11309..12361
                     /gene="ccmH"
                     /locus_tag="ACHOSC_RS11800"
                     /old_locus_tag="pgaptmp_002360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c-type biogenesis thiol:disulfide
                     oxidoreductase CcmH"
                     /protein_id="HGU4548289.1"
                     /translation="MRFLLGVLMLMISGSALATIDVLQFKDEAQEQQFRQLTEELRCP
                     KCQNNSIADSNSMIATDLRQKVYELMQEGKSKKEIVDYMVARYGNFVTYDPPLTPLTV
                     LLWVLPVVAIGIGGWVIYARSRRRVRVVPEAFPEQSVPEGKRAGYVAYLPGIVVALIV
                     AGVSYYQTGNYQQVKIWQQATAQAPALLDRALDPKADPLNEEEMSRLALGMRTQLQKN
                     PGDIEGWIMLGRVGMALGNASIATDAYATAYRLDPKNSDAALGYAEALTRSSDPNDNR
                     LGGELLRQLVRSDHSNIRVLSMYAFNAFEQQRFGEAVAAWEMMLKLLPANDTRRAVIE
                     RSIAQAMQHLSPQENK"
     gene            complement(12396..13043)
                     /gene="narP"
                     /locus_tag="ACHOSC_RS11805"
                     /old_locus_tag="pgaptmp_002361"
     CDS             complement(12396..13043)
                     /gene="narP"
                     /locus_tag="ACHOSC_RS11805"
                     /old_locus_tag="pgaptmp_002361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate/nitrite response regulator protein NarP"
                     /protein_id="HGU4548290.1"
                     /translation="MPEATPFQVMIVDDHPLMRRGVRQLLELDPGFEVVAEAGDGASA
                     IDLANRLDIDVILLDLNMKGMSGLDTLNALRRDGVTAQIIILTVSDASSDVFALIDAG
                     ADGYLLKDSDPEVLLEAIRAGAKGSKVFSERVNQYLREREMFGAEEDPFSVLTERELD
                     VLHELAQGLSNKQIASVLNISEQTVKVHIRNLLRKLNVRSRVAATILFLQQRGAQ"
     gene            13363..15954
                     /gene="yejO"
                     /locus_tag="ACHOSC_RS11810"
                     /old_locus_tag="pgaptmp_002362"
                     /pseudo
     CDS             13363..15954
                     /gene="yejO"
                     /locus_tag="ACHOSC_RS11810"
                     /old_locus_tag="pgaptmp_002362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311108.2"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter YejO"
BASE COUNT         3571 a         3942 c         4675 g         3870 t
ORIGIN      
        1 gctatataaa tatgtttata accatttgaa atgtgagcaa aagcccgttt ttgccacgct
       61 tcccgccact cttttgatcc tgctacaggt tttaccccga tcggggtatg catctttgac
      121 acatccttta atatcttagc ggctataaaa atggcttatt aattatgcgg tttatttggt
      181 cgctctcaat tttcagagcg cgttaatgat ggaaggtcaa tgtgaagatt gatgcatccc
      241 gtcggggcat actcactggt cgctggcgca aagccagtaa cggtatccgt ccgccctggt
      301 ccggtgatga atctcatttt ctgacccatt gcacccgctg cgacgcctgt attaatgctt
      361 gtgaaaacaa cattctgcaa cgcggcgcgg gcggctatcc gagcgttaat ttcaaaaata
      421 acgagtgcag cttctgttac gcctgcgcgc aggcttgccc cgaatcgcta ttttctccgc
      481 gccacaccag ggcctgggat ttgcagttca ccattgggga cgcgtgcctg gcgtatcagt
      541 cagttgaatg ccgccgctgt caggatagct gtgagccaat ggcaattatc tttcgcccga
      601 cgctctccgg gatctaccag ccgcaactta atagccaact ttgcaacgga tgtggagcat
      661 gtgcagccag ctgtccggta tcagccataa ctgcggagta tctccatgca cactaactgg
      721 caagtttgca gcctggtcgt gcaggccaaa agcgaacgaa tttcagacat cagcacccaa
      781 ctgaacgcct ttcccggctg tgaagttgct gtcagcgacg cgccgagcgg tcagttgatt
      841 gtggtggtgg aagcagaaga cagcgaaacg ctgatccaaa ccattgagtc agtacgcaac
      901 gtagagggcg tgctggcggt gtcgctggtt tatcaccagc aggaagagca aggtgaggaa
      961 acaccatgaa actcagtcgt cgtagcttta tgaaagctaa cgccgttgcg gccgctgcgg
     1021 cggctgccgg tctcagcgtg ccgggcgttg cccgcgccgt tgttggtcag caggaagcca
     1081 ttaaatggga taaagcgccg tgccgtttct gcggtactgg ttgcggcgtt ctggtcggaa
     1141 cgcagcaggg gcgtgtggtg gcctgtcagg gcgacccgga cgcaccggtt aaccgtggcc
     1201 tgaactgcat taagggctat ttcctgccca agatcatgta cggtaaagac cgtttgacgc
     1261 agccgctgct gcgtatgaaa aacggtaaat atgacaaaga aggcgaattt accccaatca
     1321 cctgggatca ggccttcgat gtgatggaag agaaattcaa aaccgccctg aaagaaaaag
     1381 gaccggaatc gatcggtatg ttcggttctg gtcagtggac tatctgggaa ggttatgccg
     1441 cgtccaagct gttcaaagcg ggcttccgtt cgaacaacat cgacccgaac gcacgtcact
     1501 gtatggcgtc ggcagtagtt ggctttatgc gtacctttgg tatggatgag ccgatgggct
     1561 gctatgacga catcgagcag gctgacgcgt ttgtgctgtg gggcgctaac atggcggaga
     1621 tgcacccgat cctctggtca cgcatcacta accgtcgtct ctctaaccag aacgtcaccg
     1681 tggcggtgct ttctacctac cagcatcgta gcttcgagct ggcggataac ggcatcatct
     1741 ttacgccaca gtctgacctg gtgatcctga actacatcgc caactatatc attcaaaaca
     1801 atgcgataaa tcaggacttc ttcagcaagc acgttaacct gcgcaaaggg gcgacggaca
     1861 tcggctacgg tttacgtccg acccatccgc tggaaaaagc agcgaagaat ccgggttctg
     1921 acgcctccga accgatgagc tttgaagatt acaaagcctt cgttgccgag tatacgctgg
     1981 aaaaaactgc cgaaatgacc ggcgtgccga aagaccagtt agaacaactg gcgcagctgt
     2041 atgccgatcc gaacaagaaa gtcatctcct actggacgat gggcttcaac cagcatactc
     2101 gtggcgtgtg ggccaacaac ctggtctaca acctgcacct gctgaccggc aaaatttccc
     2161 agccgggttg cggtccgttc tccctgaccg ggcagccttc cgcgtgtggt actgcgcgtg
     2221 aagtgggcac ctttgctcac cgtctgcctg cggacatggt ggtgactaac gagaaacacc
     2281 gtgatatttg cgagaagaag tggaatatcc cgagcggcac cattccggcg aaaatcggtc
     2341 tgcacgcggt ggcacaagac cgtgcactga aagacggcaa gctgaatgtt tactggacca
     2401 tgtgtaccaa caacatgcag gccgggccga acattaatga agagcgtatg ccgggctggc
     2461 gcgatccgcg caacttcatc atcgtctccg atccgtatcc gacagtcagt gcgctggcag
     2521 ccgacttgat cctgccgacc gcaatgtggg tagagaaaga gggcgcttac ggtaacgccg
     2581 aacgccgtac tcagttctgg cgtcagcagg tacaggcgcc aggcgaagcg aaatcggatc
     2641 tctggcagtt agtccagttc tcccgccgct tcaaaactga agaagtatgg ccggaagagc
     2701 tgctggcgaa gaaaccggaa ctgcgtggca aaacgctgta cgaagttctg tatgccaccc
     2761 ccgaagtgag caaataccct gtatccgaac tggcggaaga tcagctgaac gatgaatccc
     2821 gcgagctggg cttctatctg caaaaagggc tgttcgaaga gtacgcatgg tttggtcgcg
     2881 gtcacggtca cgatctggca ccgttcgatg actaccacaa agcgcgcggt ctgcgctggc
     2941 cggtggtgaa cggtaaagaa acgcagtggc gttacagcga aggtaacgac ccgtacgtga
     3001 aagcgggcga aggctataag ttctacggta aaccggatgg caaagcggtg atcttcgcac
     3061 tgccgttcga accggcggcg gaagcaccgg atgaagagta cgacctgtgg ctctctaccg
     3121 gacgcgttct ggagcactgg cacaccggca gtatgactcg ccgtgtgccg gaactgcacc
     3181 gcgccttccc ggaagcggtc ttgtttattc acccgctgga tgcgaaagcg cgcgatctgc
     3241 gccgtggcga caaagtgaaa gtggtttctc gccgtggcga agtgatctcg attgttgaaa
     3301 cgcgcggtcg taaccgtccg ccacagggcc tggtgtacat gccgttcttc gacgccgcac
     3361 agctggttaa caaactgacg ctggatgcga ccgatccgct ctcgaaagag acggacttca
     3421 agaagtgcgc agtcaaactg gagaaggtgt aagccattat gtcccggtca gcgaaacctc
     3481 aaaatggtcg ccgccgcttt ctgcgcgatg tcgttcgcac agcaggcggg ctggctgccg
     3541 tgggtgtggc gctggggtta caacagcaaa ccgcacgcgc atctggcgtg cggttgcgcc
     3601 cgcccggagc cataaacgag aacgcctttg ccagtgcctg tgtgcgttgc ggtcagtgtg
     3661 ttcaggcttg cccttacgac accttaaaac tggcgacgct ggcctctggt ctgtcggcgg
     3721 gcacgccata ttttgtcgca cgggatattc cttgcgaaat gtgtgaggac attccgtgcg
     3781 ccaaagtgtg cccaagcggt gcgctggatc gtgagattga atcgatcgac gacgcgcgga
     3841 tggggctggc ggtactggtg gaccaggaaa actgtctcaa ctttcagggg ctgcgctgcg
     3901 atgtttgtta tcgcgaatgc ccgaaaattg atgaggccat caccctggag ctggagcgca
     3961 acacgcgtac cggtaagcac gcccgctttc tgccgacggt tcacagcgac gcctgtaccg
     4021 gttgcggtaa gtgcgaaaaa gtatgcgtgc tggaacaacc ggcaatcaag gtgttaccgc
     4081 tgtcactggc gaaaggggag ttaggtcacc attaccgctt cggttggctg gaggggaaca
     4141 atggcaaatc gtaaacgtga cgccgggcgc gaggcgctgg agaaaaaagg ctggtggcgc
     4201 agtcaccgtt ggctggtgtt acgtcgcctt tgtcagttct tcgtgctggg gatgtttttg
     4261 agcggtccat ggtttggtgt gtggatcttg cacggcaact acagcagtag cctgttattc
     4321 gacaccgttc cgctgaccga tccgctgatg acgctgcaaa gtcttgccag tggtcatctg
     4381 cctgccacgg tggcgttgac cggtgcggtg attatcaccg tgctctacgc cctggcggga
     4441 aagcgattat tttgcagctg ggtctgcccg ctgaacccga ttaccgactt agcgaactgg
     4501 ttacgcaggc gctttgacct caatcagtct gcgacgatcc ctcgccacat tcgctacgtg
     4561 ctgttggtgg tcattctgct gggatcggcc ttaactggca cgctcatctg ggaatggatc
     4621 aaccccgtat ctctgatggg gcgcagcctg gtaatgggct tcggcagcgg cgcgctgctt
     4681 attctcgcgc tgtttttatt tgatctactg gtcgttgaac acggctggtg cgggtacatt
     4741 tgtccggttg gcgcgctgta tggcgtgctg ggtagcaaag gtgtgattac cgttgcggcc
     4801 accgatcggc agaaatgtaa ccgctgtatg gattgttttc atgtttgccc ggaaccgcat
     4861 gtgctacgtg ccccggtgct ggatgaacaa agcccggtgc aggtcaccag ccgcgattgc
     4921 atgacgtgcg gtcgctgcgt ggatgtctgt tctgaggatg tatttacaat aactacacga
     4981 tggagttcgg gagcgaaatc atgaaaagcc atgacctgaa gaaagcgctg tgtcaatgga
     5041 cggcgatgct ggccctggtg gtaagcggcg cggtttgggc ggctaatggc gtcgatttta
     5101 gccagtcgcc ggaagtatcc gggacacagg aaggggccat tcggatgcca aaagagcagg
     5161 aacggatgcc gctgaactat gtgaatcagc cgccaatgat cccgcatagc gttgaaggtt
     5221 atcaggtaac gaccaatacc aaccgctgct tgcagtgcca cggtgtcgaa agttatcgca
     5281 ccactggcgc gccgcgtatc agtcctactc actttatgga cagcgacggc aaagtgggcg
     5341 cggaagtggc tccgcgtcgt tatttctgtc tgcaatgtca cgtaccgcag gccgataccg
     5401 cgccaatcgt ggggaatacc tttaccccat caaaaggtta cgggaaataa gaggttatta
     5461 tgggaaattc tgaccgtaag cctggtctga ttaagcgcct gtggaaatgg tggcgtaccc
     5521 ccagccgtct ggcgctgggg acgctgctgt tgatcggttt tgttggcggc atcgtcttct
     5581 ggggtggctt taacaccggg atggaaaaag ccaataccga agagttctgc attagctgcc
     5641 acgaaatgcg caacacggtg tatcaggaat acatggattc cgtgcactac aacaaccgta
     5701 gcggcgtccg tgcgacctgt ccggattgtc acgttccgca cgagtttgtg ccgaagatga
     5761 tacgcaagct caaagcaagt aaagagctgt atggtaaaat ttttggcgtt attgacacgc
     5821 cgcagaaatt tgaagctcat cgtctgacga tggcacagaa tgagtggcgg cgcatgaagg
     5881 acaataactc gcaggagtgc cgtaactgtc acaacttcga gtatatggat acaaccgccc
     5941 agaaatcggt tgccgcgaag atgcatgacc aggcggtgaa agatgggcaa acctgtattg
     6001 attgccataa agggatagcg cacaagctgc ccgatatgcg tgaagtcgag ccaggttttt
     6061 aacagggtta ttgcgtgggt atgcttgaag ccagagagtt actttgtgag cgggatgaac
     6121 gaaccttatt tagtggcttg tcatttacgc tgaacgcagg agagtgggta caaatcaccg
     6181 gtagcaacgg cgcggggaag acaacgcttc tccgtttgct gacggggttg tctcgccctg
     6241 acgcaggcga ggttctctgg caagggcagc ccttgcatca ggtacgcgac agctaccatc
     6301 aaaacctgtt atggataggc catcagccgg ggatcaaaac ccggctgacg gcgttagaaa
     6361 atctgcactt ttatcatcgc gatggcgata ccgcacaatg tctggaagcc ctggcgcagg
     6421 ccgggcttgc cggattcgaa gatattcctg taaatcagct ctcggccggg caacaacgcc
     6481 gcgtcgcttt agcgcgtctg tggctgaccc gtgccacgtt atggatcctc gacgagcctt
     6541 ttaccgcgat tgacgttaac ggcgtcgatc gtctgaccca gcgtatggcg cagcatacgg
     6601 agcagggggg gattgtgatt ctgactaccc accagccgct caacgttgct gaaagtaaaa
     6661 ttcgccgcat ttcactgacg caaacgaggg ccgcatgatg ttctggcgca ttttccgtct
     6721 tgagctgcgt gtagcgtttc gccatagcgc cgaaatcgcc aacccgctgt ggttcttcct
     6781 gattgtaatt accctttttc cgctcagtat cggtccggag ccgcaactgc tggcgcgtat
     6841 tgcaccgggc attatctggg ttgctgcact gctttcatcc ttgctggcgc tggaacgact
     6901 gttccgtgac gatttgcagg acggcagtct tgaacaattg atgttgttgc cgttaccctt
     6961 gcccgccgtt gtgctggcga aggtgatggc gcactggatg gtaagcggtc tgccgttact
     7021 cattctttcg ccactggtag caatgctact gggaatggat gtttatggct ggcaagtgat
     7081 ggcgctgacg ctgctgctgg gaacgcctac gcttggcttt ctcggtgcac cgggcgtggc
     7141 gctgacagtg ggacttaagc gcggtggtgt gctgctcagc atactggtgt taccgctgac
     7201 tatcccatta ctcatcttcg ccaccgccgc gatggacgcg gcttccatgc atttgcccgt
     7261 tgacgggtat ctggcaattt taggcgcgtt gctggcaggc accgcgacat taagtccttt
     7321 tgcgacggcg gcagcgttac gaatcagcat tcaataacgc tggattactg atttttgttc
     7381 gagtctggta tcgaaactat gtggaaaaca ctgcatcaac tggcgatccc accacggctg
     7441 tatcaaatct gtggctggtt tataccgtgg ctggcaattg ccagtgtggt cgtgcttacc
     7501 gtcggctgga tctggggatt cggctttgct ccggctgatt atcagcaggg aaatagctac
     7561 cgcattatct acctgcatgt gcctgcggcg atctggtcga tgggcattta tgcatcaatg
     7621 gcagtggcag cgtttattgg ccttgtctgg cagatgaaaa tggccaacct ggcggtggcg
     7681 gcgatggccc ccattggtgc cgtgtttacc tttattgccc tggttaccgg ctctgcatgg
     7741 ggaaaaccga tgtggggcac ctggtgggta tgggatgcac gtctgacttc tgaactggtg
     7801 ctgctttttt tgtatgtggg tgtgattgcc ctgtggcacg ccttcgacga ccgccgtctg
     7861 gcgggccgtg cggcaggtat cctggtgctg attggcgtgg tgaatctgcc gattattcat
     7921 tactccgtgg agtggtggaa caccctgcat cagggatcaa cgcggatgca gcaaagtatc
     7981 gatccggcga tgcgttcgcc gctgcgctgg tcgatttttg gcttcctgct cctgtctgcc
     8041 acgctgacgc tgatgcggat gcgtaatttg attttgctga tggaaaaacg ccgtccgtgg
     8101 gtgagtgaac tgatactgaa aagaggccgt aaatgacccc tgcatttgct tcctggaatg
     8161 aatttttcgc aatgggcggt tacgcctttt ttgtctggct ggcggtggtg atgaccgtta
     8221 ttccgctggt ggttttggtc gtgcactcgg tgatgcagca tcgcgcaatt ctgcgtggtg
     8281 tggcgcaaca gcgggcgcgt gaggcgcgtt tacgtgctgc gcaacagcag gaggctgcat
     8341 gaatattcgc cgtaaaaacc gcttgtggat tgcctgtgct gttctggcag ggttggcact
     8401 gactatcggt ctggtgctat atgcgctgcg ctcgaatatc gatctctttt atacgccggg
     8461 ggaaattctc tacggcaagc gtgaaactca gcaaatgccg gaagtcggtc agcgtctgcg
     8521 cgttggcggg atggtgatgc cgggtagcgt gcagcgcgat cccaattcgc taaaagtaac
     8581 tttcaccatt tacgacgctg aaggttcagt agatgtctct tacgaaggca ttttgccgga
     8641 tctgttccgt gaagggcagg gcgttgtggt gcagggcgaa ctggaaaaag gcaatcatat
     8701 cctcgcaaaa gaagtgctgg cgaaacacga cgaaaactac acgccgccag aagttgagaa
     8761 agcgatggaa gccaatcacc gtcgcccggc gagtgtttat aaggacccag catcatgatg
     8821 ccggaaattg gtaacgggct gctgtgtctg gcgctaggaa ttgcgctgct gctgtccgtg
     8881 tatccgctat ggggcgtagc gcgcggagat gcgcgcatga tggcgtcttc ccgcttgttt
     8941 gcctggctgc tgtttatgtc tgtggctggc gcatttctgg tactggtcaa tgccttcgtg
     9001 gtcaacgact tcaccgtcac ctatgttgcc agcaactcca atacccagct tccggtgtgg
     9061 tatcgcgtgg cggctacctg gggcgcgcat gaaggctcgc tcctgctgtg ggtgctgctg
     9121 atgagcggct ggacctttgc ggtagcgatt tttagtcagc gtattccgct ggatattgtg
     9181 gcccgcgtac tggcgataat ggggatggtc agcgtcggct ttttgctgtt cattctcttt
     9241 acctctaacc cgttctcacg cacgttgccg aacttcccga ttgaagggcg cgatcttaac
     9301 ccgctgttac aggatccggg gctgatcttc catccgcctc tgctctatat ggggtacgtg
     9361 gggttctcgg tggcgtttgc ttttgccatt gcttctttgt tgtgcgggcg tctggacagc
     9421 acttatgcgc gttttactcg tccgtggacg ctggcggcgt ggatcttctt gacgctcggc
     9481 attgtgctcg gttccgcatg ggcctattac gaactcggct ggggcggctg gtggttctgg
     9541 gatccggtag aaaacgcctc gtttatgccg tggctggtgg ggactgcgct gatgcactca
     9601 ctggcggtca ctgaacaacg cgccagcttc aaagcgtgga cattactgct ggcaatcagt
     9661 gccttctcgt tgtgtctgct ggggactttc ctggtgcgtt ccggcgtgct ggtatcggta
     9721 cacgcgtttg cctctgatcc ggcacgcggt atgtttatcc tcgcctttat ggtactggtg
     9781 attggtggtt cgctgctgct gtttgccgcg cgtggacaca aagttcgctc acgcgtaaac
     9841 aatgcgctgt ggtcgcggga atctctgctg ttagcgaaca acgttttgct ggtcgccgcg
     9901 atgctggtgg tattactggg gacgctgctg ccgctggtgc acaagcaact gggactgggc
     9961 agtatttcga ttggcgaacc gttcttcaac accatgttta cctggctgat ggtgccgttt
    10021 gcgctgctgc ttggtgtcgg tcctctggtg cgctgggggc gcgatcgccc acgtaaaatc
    10081 cgcaatttat tgattatcgc cttcatctca acgctggtgc tgtcgctgct gctgccgtgg
    10141 ctgttcgaaa gcaaagttgt ggcgatgacg gtgctcggcc tggcaatggc ctgctggatt
    10201 gctgtgctgg caattgcgga agctgcgctg cgtatttcac gtggcacgaa aaccaccttc
    10261 agttattggg gaatggtggc ggctcacctg gggctggcag tgacaattgt tggtattgcc
    10321 tttagccaga actatagcgt tgagcgtgat gtgcgcatga agtccggcga tagcgtcgat
    10381 attcatgaat atcgcttcac cttccgtgat gtcaaagagg tgactggccc gaactggcgt
    10441 ggcggtgtgg cgactatcgg cgtaacgcgc gatggcaagc cggaaacggt gctgtatgcg
    10501 gaaaaacgtt attacaacac tgccgggtcg atgatgaccg aagcggcaat tgacggcggc
    10561 atcacgcgtg acctgtacgc cgcgctcggt gaagagctgg aaaacggcgc gtgggccgtg
    10621 cgtctttact acaaaccatt tgttcgctgg atttgggcgg gcgggctgat gatggcgttg
    10681 ggcggactac tgtgtctgtt tgatcctcgc tatcgtaagc gcgtgagtcc gcaaaaaact
    10741 gcgccggagg ccgtatgaag cgcaaagtat tgttaattcc gttgattatc ttcctggcga
    10801 ttgccgcggc gctgctgtgg cagctggcgc gtaatgccga aggggatgat ccgaccaatc
    10861 tggaatcggc gctcattggc aagcctgtgc cgaagtttcg tctcgaatca ctggacaatc
    10921 cggggcagtt ttatcaggcg gatgtgctga ctcagggcaa accagtactg cttaacgtct
    10981 gggcgacctg gtgtccgacc tgccgtgcgg aacatcaata tctgaatcag ctttcagcgc
    11041 agggcattcg cgtggtcggc atgaactata aagacgatcg ccagaaggca atcagctggc
    11101 tgaaagagct gggcaatcct tacgcgctaa gcctgtttga tggcgacggc atgttagggc
    11161 tggatctcgg tgtctatggc gcgccagaaa cgttccttat tgacggcaac ggcatcattc
    11221 gctatcgcca tgcgggcgat ctcaatcctc gcgtctggga agaagagatc aagccgctgt
    11281 gggagaaata cagtaaggag gccgcacaat gaggttttta ttgggcgtgc tgatgctgat
    11341 gatctccggc tcagcgctgg cgaccatcga cgtgttgcag tttaaagatg aagcacagga
    11401 acaacagttc cgtcagctca ctgaagaact gcgctgcccg aaatgccaga ataacagcat
    11461 tgccgattcc aactcgatga ttgccaccga cctgcgccag aaagtgtatg aactgatgca
    11521 ggaaggtaaa agtaagaaag agattgtcga ttatatggtg gcgcgttacg gcaacttcgt
    11581 cacttacgat ccgccgttaa cgccgctgac cgtgctgctg tgggtgctgc cagtagtggc
    11641 tattggcatt ggcggttggg tcatttacgc ccgctcgcgg cgtcgggtac gcgtggtgcc
    11701 ggaagcgttt cctgaacaaa gcgtgccgga aggtaagcgt gccggatatg tcgcttattt
    11761 gccgggcatt gtggtggcgt taattgtggc tggcgtcagc tactaccaga ccggaaatta
    11821 tcaacaggtg aaaatctggc agcaggccac ggcacaggct ccggcgttgc tggacagggc
    11881 gctggatccg aaagccgatc cgctcaacga agaagagatg tcgcgtcttg cgctggggat
    11941 gcgtactcaa ctgcaaaaaa atccgggaga tatagaaggc tggattatgt tgggccgcgt
    12001 tggcatggcg ctgggtaacg ctagtatcgc caccgatgca tacgctactg cgtatcgcct
    12061 cgatccgaaa aacagcgatg ccgcactggg ttatgctgaa gcgttgacac gttcatctga
    12121 tcccaacgac aaccgcctcg gcggcgaact gctgcgccag ttggtgagaa gcgaccacag
    12181 caatatccgt gtgttaagca tgtatgcgtt taatgccttt gagcagcagc gatttggcga
    12241 agccgttgcc gcgtgggaga tgatgttgaa actcttacct gccaacgata ctcgccgtgc
    12301 ggtgattgaa cgtagtatcg cgcaggcgat gcaacatttg tcgccgcagg agaataaata
    12361 aaaagaggcc cgatggtgat gccatcgggc tatttttatt gtgccccgcg ttgttgcagg
    12421 aacagaatgg tggctgccac gcgtgagcgg acattgagtt tgcgcagcag attgcgaata
    12481 tgtactttta ctgtctgctc tgaaatattc aacaccgagg caatctgttt atttgacagc
    12541 ccctgtgcca gctcgtgcag aacatccagc tcgcgctccg tcagcacgct gaagggatct
    12601 tcttccgcgc caaacatttc acgttcacgt aagtactgat tgacgcgttc gctaaagact
    12661 ttgctgcctt tcgctccagc acgaattgct tccagcaata cttccgggtc gctgtctttc
    12721 aacagataac cgtctgcgcc tgcgtctatc agcgcaaaga catcgctgga ggcatctgat
    12781 acggtcagga taataatttg cgcggtaacg ccatccctgc gcaaggcatt gagggtatcc
    12841 aggccactca tacctttcat attgagatcc agcaagatca cgtcgatatc cagtctattc
    12901 gccagatcga tcgcgctcgc gccgtcgccc gcttcggcga ccacttcaaa gccaggatca
    12961 agctccagta actgacgaac accgcgtcgc ataagtggat gatcatccac aatcatcacc
    13021 tgaaaaggcg ttgcttcagg catagtagtc tcctgaggtt ttattagaat cattatgttt
    13081 gagcgtagcg ttggtaacgc taccaggcgc gtgaatgtta ccccatttta tgtatggaca
    13141 tgtagtgaaa actacaacct tgtcgttcgc tttggcatca ttttcattat aaagacggaa
    13201 ttttgcatca gcttctcatc aagaaaaaca actaaaaaat caatcacaat acttgtgcat
    13261 ttctttaaac agtttttaaa aactgactcc cggtatggag ctatgggtat tttctgtacc
    13321 caatgctttt aacagcaatt aatttcatag gatgaaagct caatgcatca atctggttct
    13381 gtttctcttt gtcgttccgc aatatctgtt ctggtggcta cagcgttata ttcacccata
    13441 gcattggcat caacagttga gtatggtgag acagttgatg gtgttgtcct ggaaaaagat
    13501 atccagctgg tttatgggac cgccaataat acgaaaatca atcctggcgg agaacagcat
    13561 ataaaagaat ttggtgtaag taataatact gaaattaacg gagggtatca gtacattgaa
    13621 atgaatggcg ccgcagaata ctcagtatta aatgacggtt atcaaattgt tcaaatgggt
    13681 ggcgcggcaa accagactac gctcaataat ggtgtgctac aggtttatgg cgcagcgaat
    13741 gataccacga ttaaaggcgg gcgcttaatc gttgaaaaag atgggggggc cgtctttgtc
    13801 gctatcgaaa agggaggact actggaggtt aaagaggggg gattagcgat tgcggtagat
    13861 cagaaagcag gcggtgctat taaaacaacc acgcgggcca tggaggtatt cggaacaaac
    13921 cgtctcggtc agttcgatat caagaatggt attgctaata atatgttgtt ggaaaacggc
    13981 ggaagtttgc gagttgaaga aaatgacttc gcttataata ccactgtaga tagtggcggc
    14041 ttactggagg ttatggatgg cgggactgta actggcgttg ataaaaaagc aggcggaaaa
    14101 ttaattgtct caacgaatgc gctggaagtg agtggtccaa acagtaaagg ccaatttagt
    14161 ataaaagatg gtgtgtcaaa aaattatgaa ctggatgatg gttccgggct cattgttatg
    14221 gaggacacgc aggccattga tactatcctt gatgagcatg ccactatgca atcgctggga
    14281 aaggatactg gtacgaaagt gcaggcaaat gcggtatatg atctcggtcg atcatatcag
    14341 aatggaagta tcacgtattc ctcaaaagcc atctctgaaa atatggttat caacaatggc
    14401 cgcgctaacg tctgggctgg cacaatggtt aacgtttcag tcagagggaa tgatggcatt
    14461 cttgaggtca tgaagccgca aataaattat gcacccgcaa tgttggtggg taaggtagtg
    14521 gtttctgagg gcgcttcttt tagaacgcat agtgccgtgg ataccagcaa agcggacgtt
    14581 tcgctcgaaa atagcgtatg gaccatcatt gccgatatca ctacgacgaa ccaaaacacc
    14641 ctcctcaact tagccaacct tgcgatgtct gacgcaaatg tgattatgat ggatgagcca
    14701 gtgactcgtt catcagtgac ggcaagtgcg gaaaatttca ttacgttgac caccaatacc
    14761 ctgtcgggaa acggcaattt ttatatgcgt accgatatgg ctaatcatca gagcgatcag
    14821 ctcaacgtca ccggtcaggc aacaggtgat ttcaaaatat tcgtgacgga caccggtgcc
    14881 agcccggcag caggagatag ccttacactg gtaacaacgg gcggcggtga tgctgcattt
    14941 acgttgggca atgccggagg cgttgttgat atcggtacgt atgaatatac cttgctggat
    15001 aatggcaacc atagctggag tttggcagag aatcgcgcgc aaattacccc ttcaaccact
    15061 gatgtgctga atatggcggc agcacaaccg ctggtatttg atgcagaact ggacaccgtg
    15121 cgtgagcgtc ttggtagcgt aaaaggcgtt aattacgata cggcgatgtg gagttcggca
    15181 attaacaccc gcaacaacgt gaccactgat gcgggcgctg gctttgagca aacattgacc
    15241 gggctgacgc tcggtatcga tagccgtttc tcccgtgaag aaagcagcac aattcgcggc
    15301 ttgttctttg gttactctca ttctgatatt ggttttgatc gcggcggcaa aggcaatgtc
    15361 gatagctata ccctgggggc ttatgccggt tgggagcatc agaacggtgc ctatgttgat
    15421 ggagtggtga aagttgaccg ttttgccaac accatccatg gcaagatgag taatggggca
    15481 acagcgtttg gcgattacaa tagtaacggc gcgggtgctc atgtcgagag cgggttccgt
    15541 tgggttgacg gattgtggag tgttagaccc tatctggcct ttaccggctt taccacagat
    15601 ggtcaggact acacgttatc aaacggcatg cgcgcggatg tgggaaatac ccggatatta
    15661 cgcgctgaag cgggaacggc ggtaagctat cacatggacc tgcaaaacgg tacgacgctg
    15721 gaaccctgac tgaaagccgc cgtgcgtcag gaatacgccg attctaacca ggtgaaagtt
    15781 aatgacgatg gcaaatttaa taatgatgtg gctggaaccc gtggcgttta tcaggctggg
    15841 ataaggtcat cgtttacccc gacgttaagc ggtcatttgt cagtcagcta tggcaatggc
    15901 gcaggggtag aatcgccgtg gaatactcag gcgggtgtgg tctggacgtt ctgataacaa
    15961 aaaataaaca ggctgtgatg tgtcacggtc tgtttatcga attaattgca gatataaaaa
    16021 aaaccaaccg taaaggttgg ttttttcttg ggattttt
//
LOCUS       DAZKHF020000089        15963 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2201S31241
            SAMN43267800-rid25106413.denovo.090, whole genome shotgun sequence.
ACCESSION   DAZKHF020000089 DAZKHF020000000
VERSION     DAZKHF020000089.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267800
            Sequence Read Archive: SRR30307263
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 15963)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15963)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 15963)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350581.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350581.2
            Genome Coverage       :: 151x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:21:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,652
            CDSs (total)                      :: 5,557
            Genes (coding)                    :: 5,320
            CDSs (with protein)               :: 5,320
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 237
            CDSs (without protein)            :: 237
            Pseudo Genes (ambiguous residues) :: 0 of 237
            Pseudo Genes (frameshifted)       :: 102 of 237
            Pseudo Genes (incomplete)         :: 130 of 237
            Pseudo Genes (internal stop)      :: 52 of 237
            Pseudo Genes (multiple problems)  :: 41 of 237
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15963
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267800-rid25106413.denovo.090"
                     /serotype="O 26:H11"
                     /isolate="2201S31241"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-01"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            <1..188
                     /locus_tag="ACHOSC_RS11815"
                     /old_locus_tag="pgaptmp_002363"
     CDS             <1..188
                     /locus_tag="ACHOSC_RS11815"
                     /old_locus_tag="pgaptmp_002363"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4548291.1"
                     /translation="AKRQLNLFIMQNGIAHNGLILLWRMREGKHLCPGKTTLSDHAVL
                     YLPVSTFVGTGFFIHPL"
     gene            199..3045
                     /locus_tag="ACHOSC_RS11820"
                     /old_locus_tag="pgaptmp_002364"
     CDS             199..3045
                     /locus_tag="ACHOSC_RS11820"
                     /old_locus_tag="pgaptmp_002364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ag43/Cah family autotransporter adhesin"
                     /protein_id="HGU4548292.1"
                     /translation="MKRHLNTSYRLVWNHITGTLVVASELARSRGKRAGVAVALSLAA
                     VTSVPALAADTVVQAGETVNGGTLTNHDNQIVFGTANGMTISTGLEYGPDNEANTGGQ
                     WIQNGGIANNTTVTGGGLQRVNAGGSVSDTVISAGGGQSLQGQAVNTTLNGGEQWVHE
                     GGIATVTVINEKGWQAVKSGAMATDTVVNTGAEGEPDAENGDTGQNVYGDAVRTTINK
                     NGRQIVAAEGTANTTVVYAGGDQTVHGHALDTTLNGGYQYVHNGGTASGTVVNSDGWQ
                     IIKEGGLADFTTVNQKGKLQVNAGGTATHVTLKQGGALVTSTAATVLGSNRLGNFTVE
                     NGKADGVVLESGGRLDVLEGHSAQKTRVDDGGTLAVSAGGKATDVTMTSGGALIADSG
                     ATVEGTNASGKFSIDGISGQASGLLLENGGSFTVNAGGQAGNTTVGHRGTLTLAAGGS
                     LSGRTQLSKGASMVLNGDVVSTGDIVNAGEIHFDNQTTQDAVLSRAVAKGDSPVTFHK
                     LTTTNLTGQGGTINMRVRLDGSNTSDQLVINGGQATGKTWLAFTNVGNSNLGVATTGQ
                     GIRVVDAQNGATTEEGAFALSRPLQAGAFNYTLNRDSDEDWYLRSENAYRAEVPLYAS
                     MLTQAMDYDRILAGSRSHQTGVNGENNSVRLSIQGGHLGHDNNGGIARGATPESSGSY
                     GFVRLEGDLLRTEVAGMSLTTGVYGAAGHSSVDVKDDDGSRAGTVRDDAGSLGGYLNL
                     VHTSSGLWADIVAQGTRHSMKASSDNNDFRARGWGWLGSLETGLPFSITDNLMLEPQL
                     QYTWQGLSLDDGQDNAGYVKFGHGSAQHVRAGFRLGSHNDMNFGKGTSSRDTLRDSAK
                     HSVRELPVNWWVQPSVIRTFSSRGDMSMGTAAAGSNMTFSPSRNGTSLDLQAGLEARV
                     RENITLGVQAGYAHSVSGSSAEGYNGQATLNVTF"
     gene            3373..4191
                     /locus_tag="ACHOSC_RS11825"
                     /old_locus_tag="pgaptmp_002365"
     CDS             3373..4191
                     /locus_tag="ACHOSC_RS11825"
                     /old_locus_tag="pgaptmp_002365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF932 domain-containing protein"
                     /protein_id="HGU4548293.1"
                     /translation="MRLASRFGHVNQIRRDRPLTHEELMHYVPSIFGEDRHTSRSERY
                     AYIPTITVLESLQREGFQPFFAYQIRVRDPGRRGYTKHMLRLRRDGEINGQHVPEIIL
                     LNSHDGTSSYQMLPGYFRFVCQNGCVCGQSLGEVRVPHRGNVVEKVIEGAYEVVGVFD
                     RIEEKRDAMQSLVLPPPSRQALAQAALTYRYGDEHQPVTTADILTPRRREDYGKDLWS
                     AYQTIQENMLKGGISGRSARGKRIHTRTIHSIDTDIKLNRALWVMAETLLESMR"
     gene            4283..4768
                     /locus_tag="ACHOSC_RS11830"
                     /old_locus_tag="pgaptmp_002366"
     CDS             4283..4768
                     /locus_tag="ACHOSC_RS11830"
                     /old_locus_tag="pgaptmp_002366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antirestriction protein"
                     /protein_id="HGU4548294.1"
                     /translation="MTTVTHNSTTPSVSVTAASGNNPPQLVATLVPDEQRISFWPQHF
                     GLIPQWVTLEPRVFGWMDRLCEDYCGGIWNLYTLNNGGAFMAPEPDDDDDETWVLFNA
                     MNGNRAEMSPEAAGIAACLMTYSHHACRMENYAMTVHYYRLRDYALQHPECSAIMRII
                     D"
     gene            4784..5260
                     /locus_tag="ACHOSC_RS11835"
                     /old_locus_tag="pgaptmp_002367"
     CDS             4784..5260
                     /locus_tag="ACHOSC_RS11835"
                     /old_locus_tag="pgaptmp_002367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RadC family protein"
                     /protein_id="HGU4548295.1"
                     /translation="MQQLSFLPGEMTPGERSLIQRALKTLDRHLHEPGVAFTSTRAAR
                     EWLILNMAGLEREEFRVLYLNNQNQLIAGETLFTGTINRTEVHPREVIKRALYHNAAA
                     VVLAHNHPSGEVTPSKADRLITERLVQALGLVDIRVPDHLIVGGNQVFSFAEHGLL"
     gene            5323..5544
                     /locus_tag="ACHOSC_RS11840"
                     /old_locus_tag="pgaptmp_002368"
     CDS             5323..5544
                     /locus_tag="ACHOSC_RS11840"
                     /old_locus_tag="pgaptmp_002368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF987 domain-containing protein"
                     /protein_id="HGU4548296.1"
                     /translation="MKIITRGEAMRIHQQHPTSRLFPFCTGKYRWHGSAEAYTGREVQ
                     DIPGVLAVFAERRKDSFGPYVRLMSVTLN"
     gene            5618..6025
                     /locus_tag="ACHOSC_RS11845"
                     /old_locus_tag="pgaptmp_002369"
     CDS             5618..6025
                     /locus_tag="ACHOSC_RS11845"
                     /old_locus_tag="pgaptmp_002369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001389100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV toxin-antitoxin system YeeU family
                     antitoxin"
                     /protein_id="HGU4548297.1"
                     /translation="MSDTLHGTTLPDDNHDRPWWGLPCTVTPCFGARLVQEGNRLHYL
                     ADRAGIRGRFSDADAYHLDQAFPLLMKQLELMLTSGELNPRHQHTITLYAKGLTCEAD
                     TLGSCSFVYMAVYATRFSNQLPKASVHQLTHYF"
     gene            complement(6566..7813)
                     /locus_tag="ACHOSC_RS11850"
                     /old_locus_tag="pgaptmp_002370"
     CDS             complement(6566..7813)
                     /locus_tag="ACHOSC_RS11850"
                     /old_locus_tag="pgaptmp_002370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005351 - carbohydrate:proton symporter
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharide MFS transporter"
                     /protein_id="HGU4548298.1"
                     /translation="MALNIPFRNAYYRFASSYSFLFFISWSLWWSLYAIWLKGHLGLT
                     GTELGTLYSVNQFTSILFMMFYGIVQDKLGLKKPLIWCMSFILVLTGPFMIYVYEPLL
                     QSNFSVGLILGALFFGLGYLAGCGLLDSFTEKMARNFHFEYGTARAWGSFGYAIGAFF
                     AGIFFSISPHINFWLVSLFGAVFMMINMCFKDKDHQCVAADAGGVKKEDFIAVFKDRN
                     FWVFVIFIVGTWSFYNIFDQQLFPVFYAGLFESHDVGTRLYGYLNSFQVVLEALCMAI
                     IPFFVNRVGPKNALLIGVVIMALRILSCALFVNPWIISLVKLLHAIEVPLCVISVFKY
                     SVANFDKRLSSTIFLIGFQIASSLGIVLLSTPTGILFDHAGYQTVFFAISGIVCLMLL
                     FGIFFLSKKREQIVMETPVPSAI"
     gene            complement(7885..8799)
                     /locus_tag="ACHOSC_RS11855"
                     /old_locus_tag="pgaptmp_002371"
     CDS             complement(7885..8799)
                     /locus_tag="ACHOSC_RS11855"
                     /old_locus_tag="pgaptmp_002371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016301 - kinase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminoimidazole riboside kinase"
                     /protein_id="HGU4548299.1"
                     /translation="MSAKVWVLGDAVVDLLPESDGRLLPCPGGAPANVAVGIARLGGI
                     SGFIGRVGDDPFGALMQRTLLTEGVDITYLKQDEWHRTSTVLVDLNDQGERSFTFMVR
                     PSADLFLETTDLPCWRHGEWLHLCSIALSAEPSRTSAFTAMTAIRHAGGFVSFDPNIR
                     EDLWQDEHLLRLCLRQALQLADVVKLSEEEWRLISGKTQNDQDICALAKEYEIAMLLV
                     TKGAEGVVVCYRGQVHHFAGMSVNCVDSTGAGDAFVAGLLTGLSSTGLSTDEREMRRI
                     IDLAQRCGALAVTAKGAMTALPCRQELE"
     gene            9015..10448
                     /gene="cscA"
                     /locus_tag="ACHOSC_RS11860"
                     /old_locus_tag="pgaptmp_002372"
     CDS             9015..10448
                     /gene="cscA"
                     /locus_tag="ACHOSC_RS11860"
                     /old_locus_tag="pgaptmp_002372"
                     /EC_number="3.2.1.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708234.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sucrose-6-phosphate hydrolase"
                     /protein_id="HGU4548300.1"
                     /translation="MTQSRLHAAQNALAKLHEHRGNTFYPHFHLAPPAGWMNDPNGLI
                     WFNDRYHAFYQHHPMSEHWGPMHWGHATSDDMIHWQHEPIALAPGDDNDKDGCFSGSA
                     VDDNGVLSLIYTGHVWLDGAGNDDAIREVQCLATSRDGIHFEKQGVILTPPEGIMHFR
                     DPKVWREADTWWMVVGAKDPGNTGQILLYRGSSLREWTFDRVLAHADAGESYMWECPD
                     FFSLGDQHYLMFSPQGMNAEGYSYRNRFQSGVIPGMWSPGRLFAQSGHFTELDNGHDF
                     YAPQSFLAKDGRRIVIGWMDMWESPMPSKREGWAGCMTLARELSESNGKLLQRPVHEA
                     ESLRQQHQSVSPRTISNKYVLQENAQAVEIQLQWALKNSDAEHYGLQLGTGMRLYIDN
                     QSERLVLWRYYPHENLDGYRSIPLPQRDTLALRIFIDTSSVEVFINDGEAVMSSRIYP
                     QPEERELSLYASHGVAVLQHGALWLLG"
     gene            complement(10456..11451)
                     /locus_tag="ACHOSC_RS11865"
                     /old_locus_tag="pgaptmp_002373"
     CDS             complement(10456..11451)
                     /locus_tag="ACHOSC_RS11865"
                     /old_locus_tag="pgaptmp_002373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005088955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HGU4548301.1"
                     /translation="MASLKDVARLAGVSMMTVSRVMHNAESVRPATRDRVLQAIQTLN
                     YVPDLSARKMRAQGRKPSTLAVLAQDTATTPFSVDILLAIEQTASEFGWNSFLINIFS
                     EDDAARAARQLLAHRPDGIIYTTMGLRHITLPESLYGENIVLANCVADDPALPSYIPD
                     DYTAQYESTQHLLAAGYRQPLCFWLPESALATGYRRQGFEQAWRDAGRDLAEVKQFHM
                     ATGDDHYTDLASLLNDHFKSGKPDFDVLICGNDRAAFVAYQVLLAKGVRIPQDVAVMG
                     FDNLVGVGHLFLPPLTTIQLPHDIIGREAALHIIEGREGGRVTRIPCPLLIRCST"
     gene            11694..11912
                     /locus_tag="ACHOSC_RS11870"
                     /old_locus_tag="pgaptmp_002374"
                     /pseudo
     CDS             11694..11912
                     /locus_tag="ACHOSC_RS11870"
                     /old_locus_tag="pgaptmp_002374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416866.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="D-serine transporter DsdX"
     gene            11930..13258
                     /gene="dsdA"
                     /locus_tag="ACHOSC_RS11875"
                     /old_locus_tag="pgaptmp_002375"
     CDS             11930..13258
                     /gene="dsdA"
                     /locus_tag="ACHOSC_RS11875"
                     /old_locus_tag="pgaptmp_002375"
                     /EC_number="4.3.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008721 - D-serine ammonia-lyase activity
                     [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0046416 - D-amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-serine ammonia-lyase"
                     /protein_id="HGU4548302.1"
                     /translation="MENAKMNSLIAQYPLVKDLVALKETTWFNPGTTSLAEGLPYVGL
                     TEQDVQDAHARLSRFAPYLAKAFPETAATGGIIESELVAIPAMQKRLEKEYQQPISGQ
                     LLLKKDSHLPISGSIKARGGIYEVLAHAEKLALEAGLLTLDDDYSKLLSPEFKQFFSQ
                     YSIAVGSTGNLGLSIGIMSARIGFKMTVHMSADARAWKKAKLRSHGVTVVEYEQDYGV
                     AVEEGRKAAQSDPNCFFIDDENSRTLFLGYSVAGQRLKAQFAQQGRIVDADNPLFVYL
                     PCGVGGGPGGVAFGLKLAFGDHVHCFFAEPTHSPCMLLGVHTGLHDQISVQDIGIDNL
                     TAADGLAVGRASGFVGRAMERLLDGFYTLSDQTMYDMLGWLAQEEGIRLEPSALAGMA
                     GPQRVCASVSYQQMHGFSAEQLRNATHLVWATGGGMVPEEEMNQYLAKGR"
     gene            complement(13366..14904)
                     /gene="emrY"
                     /locus_tag="ACHOSC_RS11880"
                     /old_locus_tag="pgaptmp_002376"
     CDS             complement(13366..14904)
                     /gene="emrY"
                     /locus_tag="ACHOSC_RS11880"
                     /old_locus_tag="pgaptmp_002376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter permease
                     subunit EmrY"
                     /protein_id="HGU4548303.1"
                     /translation="MAITKSTPAPLTGGTLWCVTIALSLATFMQMLDSTISNVAIPTI
                     SGFLGASTDEGTWVITSFGVANAIAIPVTGRLAQRIGELRLFLLSVTFFSLSSLMCSL
                     STNLDVLIFFRVVQGLMAGPLIPLSQSLLLRNYPPEKRTFALALWSMTVIIAPICGPI
                     LGGYICDNFSWGWIFLINVPMGIIVLTLCLTLLKGRETETSPVKMNLPGLTLLVLGVG
                     GLQIMLDKGRDLDWFNSSTIIILTVVSVISLISLVIWESTSENPILDLSLFKSRNFTI
                     GIVSITCAYLFYSGAIVLMPQLLQETMGYNAIWAGLAYAPIGIMPLLISPLIGRYGNK
                     IDMRLLVTFSFLMYAVCYYWRSVTFMPTIDFTGIILPQFFQGFAVACFFLPLTTISFS
                     GLPDNKFANASSMSNFFRTLSGSVGTSLTMTLWGRRESLHHSQLTATIDQFNPVFNSS
                     SQIMDKYYGSLSGVLNEINNEITQQSLSISANEIFRMAAIAFILLTVLVWFAKPPFTA
                     KGVG"
     gene            complement(14904..>15963)
                     /gene="emrK"
                     /locus_tag="ACHOSC_RS11885"
                     /old_locus_tag="pgaptmp_002377"
     CDS             complement(14904..>15963)
                     /gene="emrK"
                     /locus_tag="ACHOSC_RS11885"
                     /old_locus_tag="pgaptmp_002377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="multidrug efflux MFS transporter periplasmic
                     adaptor subunit EmrK"
                     /protein_id="HGU4548304.1"
                     /translation="SMELEDMISTDDAYVTGNADPISAQVSGSVTVVNHKDTNYVRQG
                     DILVSLDKTDATIALNKAKNNLANIVRQTNKLYLQDKQYSAEVASARIQYQQSLEDYN
                     RRVPLAKQGVISKETLEHTKDTLISSKAALNAAIQAYKANKALVMNTPLNRQPQVVEA
                     ADATKEAWLALKRTDIRSPVTGYIAQRSVQVGETVSPGQSLMAVVPARQMWVNANFKE
                     TQLTDVRIGQSVNIISDLYGENVVFHGRVTGINMGTGNAFSLLPAQNATGNWIKIVQR
                     VPVEVSLDPKELMEHPLRIGLSMTATIDTKNEDIAEMPDLASTVTSMPAYTSKALVID
                     TSPIEKEISNIISHNGQL"
BASE COUNT         4209 a         4057 c         3927 g         3770 t
ORIGIN      
        1 ctgcgaaaag gcagctgaat ctcttcatca tgcagaacgg aattgcacac aacggactga
       61 tacttctctg gcggatgaga gaggggaaac atttatgtcc gggaaaaacc actttatctg
      121 accatgctgt tttgtacctg ccggtatcca cttttgtggg taccggcttt tttattcacc
      181 ctctgtaagg aaaagctgat gaaacgacat ctgaacacca gctacaggct ggtatggaat
      241 cacattacgg gcaccctggt ggtggcctcc gaactggccc gctcacgggg aaaacgcgcc
      301 ggtgtggcgg ttgcgctgtc tcttgctgct gtcacatcag tcccggcact ggctgctgac
      361 acggttgtac aggcgggaga aaccgtgaac ggcggaacac tgacaaatca tgacaaccag
      421 attgtcttcg gtacggccaa cggaatgacc atcagtaccg gtctggagta tgggccggat
      481 aacgaggcca ataccggcgg acaatggata caaaatggcg gtatcgccaa caacactact
      541 gtcaccggtg gtggtcttca gagagtgaat gccggaggaa gcgtttcaga cacggttatc
      601 agtgccggag gcggacagag ccttcagggg caggcagtga acaccactct gaacggcggt
      661 gagcagtggg tacatgaagg cgggattgca acggttaccg tcattaatga gaagggctgg
      721 caggccgtca aatccggcgc aatggcaacc gacacggttg tgaataccgg cgcggaaggg
      781 gaaccggatg cagaaaatgg tgataccggg cagaacgtct acggagatgc cgtacgcacc
      841 accatcaata aaaatggtcg tcagattgtg gctgctgaag gaacggcaaa taccactgtg
      901 gtttatgccg gcggcgacca gactgtacat ggtcacgcac tggataccac gctgaatggg
      961 gggtaccagt atgtgcacaa cggcggtacg gcgtctggca ctgttgtaaa cagtgacggc
     1021 tggcagatta tcaaggaagg tggtctggcg gatttcacca ccgttaacca gaaaggcaaa
     1081 ctgcaggtga acgccggtgg taccgccaca catgtcaccc tgaaacaggg tggagcactg
     1141 gtcaccagta ctgcggcaac ggttcttggc agcaaccgtc tgggcaattt cacggtggaa
     1201 aacggtaagg ctgacggcgt tgttctggag tccggcggtc gcctggatgt actggaaggc
     1261 cattcagcgc agaaaacacg ggtggatgac ggcggtaccc tggcagtgtc tgccggtggt
     1321 aaggcaacag atgtcaccat gacatccggt ggtgccctga tagcagacag tggtgccact
     1381 gttgagggga ccaatgccag cggtaagttc agtattgatg gcatatccgg tcaggccagc
     1441 ggcctgctac tggaaaatgg cggcagcttt acggttaatg ccgggggaca ggcaggcaac
     1501 accactgtcg gacatcgtgg aacactgacg ctggctgccg ggggaagtct gagtggcaga
     1561 acacagctca gtaaaggcgc cagtatggta ctgaatggtg atgtggtcag taccggcgat
     1621 attgttaacg caggagagat tcactttgat aatcagacga cacaggatgc cgtgctgagc
     1681 cgtgctgttg caaaaggcga ctccccggta acgttccata aactgaccac caccaacctc
     1741 actggtcagg gcggcaccat caatatgcgt gttcgccttg atggcagtaa tacctcagac
     1801 cagctggtga ttaatggtgg tcaggcaacc ggcaaaacct ggcttgcgtt cacaaatgtc
     1861 ggaaacagta acctcggggt ggcaaccacc ggacagggta tccgggttgt ggatgcacag
     1921 aatggtgcca ccacagaaga aggtgcgttt gccctgagcc gcccgcttca ggccggcgcc
     1981 tttaactaca ccctgaaccg tgacagcgat gaagactggt acctgcgcag tgaaaatgct
     2041 tatcgtgctg aagtccccct gtatgcctcc atgctgacac aggcaatgga ctatgaccgg
     2101 attctggctg gctcccgcag ccatcagacc ggtgtaaacg gtgaaaataa cagcgtccgt
     2161 ctcagcattc agggcggtca tctcgggcac gataacaacg gcggtattgc ccgtggggcc
     2221 acgccggaaa gcagcggcag ctatggcttc gtccgtctgg agggtgacct gctcagaaca
     2281 gaggttgccg gtatgtctct gacgacaggg gtgtatggtg ctgcaggcca ttcttccgtt
     2341 gatgttaagg atgatgacgg ctcccgcgcc ggcacggtcc gggatgatgc cggcagtctg
     2401 ggcggatacc tgaatctggt acacacatcc tccggcctgt gggctgacat tgtggcacag
     2461 ggaacccgcc acagcatgaa agcatcatcg gacaataacg acttccgcgc ccggggctgg
     2521 ggctggctgg gctcactgga aaccggtctg cccttcagta tcactgacaa tctgatgctg
     2581 gagccacaac tgcagtacac ctggcaggga ctctccctgg atgacggcca ggataacgcc
     2641 ggttatgtga agttcgggca tggcagtgca caacatgtgc gtgccggctt ccgtctgggc
     2701 agccacaacg atatgaactt tggtaaaggc acctcatccc gtgacaccct gcgcgacagt
     2761 gccaaacaca gtgtgcgtga attgccggtg aactggtggg tacagccttc tgttatccgc
     2821 accttcagct cccggggaga catgagcatg ggtacagccg cagccggcag taacatgacg
     2881 ttctcaccgt cccggaatgg cacgtcactg gacctgcagg ccggactgga agcccgtgtc
     2941 cgggaaaata tcaccctggg cgttcaggcc ggttatgccc acagtgtcag cggcagcagc
     3001 gctgaaggtt ataacggtca ggccacactg aacgtgactt tctgataatc aggtcacttc
     3061 ccttcaggcc ccggtcatta tgaccggggc cttttacttt cagggatcaa cagatatgcc
     3121 gggttgtgca tcacgtttat tgcccgaaga cccttttact gccaaatggc atctgaattg
     3181 atcacagtct ggccagttat tacccgatcc cgatggggat attgggtgat ctccttccat
     3241 gaaaatacgc agctcaccag tggagggctt ctcagacaat aaacgtcatt catttcatct
     3301 gtcaggccgc gtcttctccg ggagacgcgg ctttttcatt tataccgcta actcattcat
     3361 aaggagcaaa taatgcgatt agcaagtcgt tttggtcatg taaaccagat acgccgtgac
     3421 cgcccgctga cacatgaaga actgatgcac tatgtgccca gtatttttgg ggaagaccgg
     3481 catacctccc gcagtgaacg ctatgcgtac attcccacca tcaccgtact agaaagcctg
     3541 cagcgggagg gctttcagcc cttcttcgcc taccagatcc gcgtgcgcga cccgggccgc
     3601 cggggataca ccaaacacat gctgcgtctg cgccgtgacg gggagataaa cggacaacat
     3661 gtccctgaaa tcatcctgct gaactcccat gacggtactt ccagctacca gatgctgccg
     3721 ggttacttca ggttcgtctg ccagaacggg tgcgtctgtg gccagtctct gggggaagtg
     3781 cgtgttccac accggggaaa tgtggtggag aaagttatcg aaggggctta cgaggtggtg
     3841 ggcgtgtttg accggataga ggagaagcgt gatgccatgc agtcgctggt cctgccgcca
     3901 ccgtcacgcc aggcgctggc acaggcggca ctgacttacc gttatggtga cgaacatcag
     3961 cccgtcacca ccgccgacat tctgacgcca cgacgccggg aggattacgg taaggacctg
     4021 tggagtgctt atcagaccat ccaggagaat atgctgaaag gcgggatttc cggtcgcagt
     4081 gccagaggaa aacgtatcca tacccgtacc attcacagca tcgacaccga cattaagctc
     4141 aaccgggcgt tgtgggtgat ggcagaaaca ctgctggaga gcatgcgctg atgccttttc
     4201 cctgaaagcg cagtcctgac tcacttccgt ccctgcctcc ggacattctt ccattcattt
     4261 actttttata aggaataatc tcatgacaac cgttacgcat aattccacca caccttctgt
     4321 ttccgtaacc gctgcatcag ggaataaccc acctcagctg gttgctactc tcgtccctga
     4381 tgaacagcgc atcagcttct ggccgcagca ttttggcctc attccacagt gggtgaccct
     4441 ggagccccgt gtcttcggct ggatggaccg tctgtgcgaa gactactgcg gtggtatctg
     4501 gaatctgtac accctgaaca acggcggggc atttatggca cccgaaccgg atgacgatga
     4561 tgacgaaaca tgggtactgt tcaatgccat gaacggtaac cgcgctgaaa tgagcccgga
     4621 agccgccggt attgccgcct gtctgatgac gtacagtcat catgcctgtc gtatggagaa
     4681 ttatgccatg acggtccatt attaccggtt gcgggattac gccctgcagc atccggaatg
     4741 cagcgccatt atgcgcatca tcgactgaat ggagagaagc acaatgcaac aactttcctt
     4801 tctgcccgga gagatgacgc ccggcgagcg cagcctcatt caacgggccc tgaaaaccct
     4861 ggaccgccat cttcatgaac ccggcgtggc cttcacctcc acccgtgcag cacgggaatg
     4921 gctgattctg aacatggcgg gactggagcg tgaagaattc cgggtgctgt atctgaacaa
     4981 ccagaatcag ctgattgccg gtgaaaccct cttcaccggc accatcaacc gcacggaagt
     5041 ccatccccgg gaagtgatta aacgcgccct gtaccacaat gccgctgccg tggtactggc
     5101 acacaatcac ccgtccggtg aagtcacacc cagtaaggca gaccggctta tcaccgaacg
     5161 tctggtacag gcactgggcc tggtggatat ccgggtgccg gaccatctga tagtcggtgg
     5221 caaccaggtt ttctcctttg ccgaacatgg tctgctttaa cccgtcacaa ccacatcaca
     5281 cctgttttca cttttatctt ctgtcttcag aggtatccca ttatgaaaat tatcacccgt
     5341 ggtgaagcca tgcgtattca ccaacaacat ccgacatccc gtctttttcc gttctgtacc
     5401 ggtaaatacc gctggcacgg cagtgctgaa gcgtataccg gtcgtgaagt gcaggatatt
     5461 cccggtgtgc tggccgtgtt tgctgaacgc cgtaaggaca gttttggtcc gtatgtccgg
     5521 ctgatgagcg tcaccctgaa ctgagtggga attctgatga gcagaattat cgccactacc
     5581 ggaccattct tagccgattt tctgtaagga ttttatcgtg tcagacacac tccacgggac
     5641 aacacttccc gacgacaatc acgaccgccc ctggtggggg ctgccctgca ccgtgacgcc
     5701 ctgtttcggg gcacgtctgg tgcaggaagg taaccggttg cattaccttg ccgaccgcgc
     5761 cggtatcaga ggccggttca gcgatgcgga tgcataccat ctggaccagg cctttccact
     5821 gctgatgaaa caactggaac tcatgctcac cagcggtgaa ctgaatcccc gccatcagca
     5881 taccatcacg ctgtatgcaa aagggctgac ctgtgaagct gatactctcg gcagttgtag
     5941 tttcgtttat atggctgttt atgctacccg attctcaaat caattaccga aagcttctgt
     6001 tcatcaacta actcattact tttaatgatg gggccctttc tggggcccaa gaaattaaca
     6061 acgctaacaa acaacataaa taacaataaa ttaaataaaa aatttcattc ctgcagggga
     6121 caccagatac ccttcaaacg aaatctacct tcaccccgta aaagataggt ttggcagcac
     6181 acttgcctta tatctactca tttttactgc aacaggttga aatctcagca ctgtcagaaa
     6241 gcgctgatga ctaaacagcc ctgggccggg cgatgtaacc atcacacaga atcctgatag
     6301 cgaaatatgg cgtgactcga tacttcactc cgcaatgcat tccttgatga attcgcagga
     6361 ccgtgataca cgggacaggt cgctgaatga cgacaatgtc ctggaaatca gcgaaccgcg
     6421 catctgaagt acatttgagc gactgtacca gaacatgaat gaggcgtttg gattaggtga
     6481 ttattagcag ggctaagcac tttagtatta ttattttccg gttgagggat agggagatat
     6541 cgacaacaac cggaaaagtt tacgtctata ttgctgaagg tacaggcgtt tccataacta
     6601 tttgctcgcg ttttttactc aggaagaaaa tgccaaatag caacatcagg cagacaatac
     6661 ccgaaattgc gaagaaaact gtctggtagc ctgcgtggtc aaagagtatc ccagtcggcg
     6721 ttgaaagcag cacaatccca agcgaactgg caatttgaaa accaatcaga aagatcgtcg
     6781 acgacaggcg cttatcaaaa tttgccacgc tgtatttgaa gacggatatg acacaaagtg
     6841 gaacctcaat agcatgtaac agcttcacta atgaaataat ccaggggtta acgaacagcg
     6901 cgcaggaaag gatacgcaac gccataatca caacaccgat aagtaatgca ttttttggcc
     6961 ctacccgatt cacaaagaaa ggaataatcg ccatgcatag cgcttcgagt accacctgga
     7021 atgagttgag ataaccatac aggcgcgttc ctacatcgtg tgattcgaat aaacctgcat
     7081 aaaagacagg aaaaagttgt tgatcaaaaa tgttatagaa agaccacgtc cccacaataa
     7141 atatgacgaa aacccagaag tttcgatcct tgaaaactgc gataaaatcc tcttttttta
     7201 cccctcccgc atccgccgct acgcactggt gatccttatc tttaaaacac atgttgatca
     7261 tcataaatac agcgccaaat agcgagacca accagaagtt gatatgggga ctgatactaa
     7321 aaaatatgcc ggcaaagaac gcgccaatag catagccaaa agatccccag gcgcgcgctg
     7381 ttccatattc gaaatgaaaa tttcgcgcca ttttttcggt gaagctgtca agcaaaccgc
     7441 atcccgccag ataccccagg ccaaaaaaga gcgcccccag aattagacct acagaaaaat
     7501 tgctttgcag taacggttca taaacgtaaa tcataaacgg tccggtcaag accaggatga
     7561 aactcataca ccagatgagc ggtttcttca gaccgagttt atcctgaacg atgccgtaga
     7621 acatcataaa tagaatgctg gtaaactggt tgaccgaata aagtgtacct aattccgtcc
     7681 ctgtcaaccc tagatgtcct ttcagccaaa tagcgtataa cgaccaccac agcgaccagg
     7741 aaataaaaaa gagaaatgag taactggatg caaaacgata gtacgcattt ctgaatggaa
     7801 tattcagtgc cataattacc tacctgtcgt taaaaaattc acgtcctatt tagagataag
     7861 agcgacttcg ccgtttactt ctcactattc cagttcttgt cgacatggca gcgctgtcat
     7921 tgcccctttc gccgttactg caagcgctcc gcaacgttga gcgagatcga taattcgtcg
     7981 catttctctc tcatctgtag ataatcccgt agaggacaga cctgtgagta acccggcaac
     8041 gaacgcatct cccgcccccg tgctatcgac acaattcaca gacattccag caaaatggtg
     8101 aacttgtcct cgataacaga ccaccacccc ttctgcacct ttagtcacca acagcatggc
     8161 gatctcatac tcttttgcca gggcgcatat atcctgatcg ttctgtgttt ttccactgat
     8221 aagtcgccat tcttcttccg agagcttgac gacatccgcc agttgtagcg cctgccgcaa
     8281 acacaagcgg agcaaatgct cgtcttgcca tagatcttca cgaatattag gatcgaagct
     8341 gacaaaacct ccggcatgcc ggatcgccgt catcgcagta aatgcgctgg tacgcgaagg
     8401 ctcggcagac aacgcaattg aacagagatg taaccattcg ccatgtcgcc agcagggcaa
     8461 gtctgtcgtc tctaaaaaaa gatcggcact ggggcggacc ataaacgtaa atgaacgttc
     8521 cccttgatcg ttcagatcga caagcaccgt ggatgtccgg tgccattcat cttgcttcag
     8581 atacgtgata tcgactccct cagttagcag cgttctttgc attaacgccc caaaaggatc
     8641 atcaccgacc cgacctataa atccacttat tccgcctaat ctggcgattc ccaccgcaac
     8701 gttagctggc gcgccgccag gacaaggcag taggcgcccg tctgattctg gcaagagatc
     8761 tacgaccgca tcccctaaaa cccatacttt ggctgacatt tttttccctt aaattcatct
     8821 gagttacgca tagtgataaa cctctttctc gcaaaatcgt catggattta ctaaaacatg
     8881 catattcgat cacaaaacgt catagttaac gttaacattt gtgatattca tcgcatttat
     8941 gaaagtaagg gactatattt ttataaaagt taacgttaac aattcaccaa atttgcttaa
     9001 ccaggatgat taaaatgacg caatctcgat tgcatgcggc gcaaaacgca ctagcaaaac
     9061 ttcatgagca ccggggtaac actttctatc cccattttca cctcgcgcca cctgccggat
     9121 ggatgaacga tccaaacggc ctgatctggt ttaacgatcg ttatcacgcg ttttatcaac
     9181 atcacccgat gagcgaacac tggggaccaa tgcactgggg acatgccacc agcgacgata
     9241 tgatccactg gcagcatgag cctattgcgc tagcgccagg agacgataat gacaaagacg
     9301 ggtgtttttc aggtagtgct gtcgatgaca atggtgtcct ctcacttatc tacaccggac
     9361 acgtctggct cgatggtgca ggtaatgacg atgcaattcg cgaagtacaa tgtctggcta
     9421 ccagtcggga tggtattcat ttcgagaaac agggtgtgat cctcactcca ccagaaggaa
     9481 tcatgcactt ccgcgatcct aaagtgtggc gtgaagccga cacatggtgg atggtagtcg
     9541 gggcgaaaga tccaggcaac acggggcaga tcctgcttta tcgcggcagt tcgttgcgtg
     9601 aatggacctt cgatcgcgta ctggcccacg ctgatgcggg tgaaagctat atgtgggaat
     9661 gtccggactt tttcagcctt ggcgatcagc attatctgat gttttccccg cagggaatga
     9721 atgccgaggg atacagttac cgaaatcgct ttcaaagtgg cgtaataccc ggaatgtggt
     9781 cgccaggacg actttttgca caatccgggc attttactga acttgataac gggcatgact
     9841 tttatgcacc acaaagcttt ttagcgaagg atggtcggcg tattgttatc ggctggatgg
     9901 atatgtggga atcgccaatg ccctcaaaac gtgaaggatg ggcaggctgc atgacgctgg
     9961 cgcgcgagct atcagagagc aatggcaaac ttctacaacg cccggtacac gaagctgagt
    10021 cgttacgcca gcagcatcaa tctgtctctc cccgcacaat cagcaataaa tatgttttgc
    10081 aggaaaacgc gcaagcagtt gagattcagt tgcagtgggc gctgaagaac agtgatgccg
    10141 aacattacgg attacagctc ggcactggaa tgcggctgta tattgataac caatctgagc
    10201 gacttgtttt gtggcggtat tacccacacg agaatttaga cggctaccgt agtattcccc
    10261 tcccgcagcg tgacacgctc gccctaagga tatttatcga tacatcatcc gtggaagtat
    10321 ttattaacga cggggaagcg gtgatgagta gtcgaatcta tccgcagcca gaagaacggg
    10381 aactgtcgct ttatgcctcc cacggagtgg ctgtgctgca acatggagca ctctggctac
    10441 tgggttaaca taatatcagg tggaacaacg gatcaacagc gggcaaggga tccgcgtcac
    10501 tcttccccct tcacgacctt caataatatg caatgcagct tcccgcccga taatgtcatg
    10561 tggaagctga attgtggtca gcggcggtaa aaacagatgc ccgacgccaa ccagattatc
    10621 aaagcccatt acggcgacat cctgcgggat tcgtaccccc ttcgccagaa gaacctgata
    10681 agccacaaag gctgcgcgat cgttaccaca tatcagaaca tcaaaatctg gtttgcccga
    10741 tttgaagtgg tcattgagta aacttgcgag atcggtgtag tgatcatcac ctgttgccat
    10801 gtgaaattgt ttcacctcag ccagatctcg tccagcatca cgccaggcct gctcaaatcc
    10861 ctgccgacga taccctgttg ccaacgcact ttccggtagc cagaagcata acggttgacg
    10921 atagcccgcc gcgagcaaat gctgtgttga ttcatattgt gcagtgtaat catcagggat
    10981 ataactgggt aacgctgggt catccgccac acagttcgcc aatacaatat tttcaccata
    11041 cagagactca ggcagcgtga tatgtcgcag ccccattgta gtatagataa tgccatccgg
    11101 acggtgggca agcagctgac gtgccgcgcg ggcagcgtca tcttcagaaa aaatattgat
    11161 taaaaaacta ttccagccga actcgctggc ggtttgctca atggcaagca gaatatcaac
    11221 agagaaagga gtggtagccg tgtcctgcgc cagcacggcg agagtcgacg gcttacgtcc
    11281 ttgagcgcgc atcttacggg cggaaagatc aggaacataa ttcagggtct ggattgcctg
    11341 caatacgcgg tcacgcgttg caggacgcac agattctgca ttatgcatca cccgggagac
    11401 tgtcatcatc gacactcccg ccaggcgtgc gacatccttt aatgaagcca tacccaagcc
    11461 gtttgccgta aaacgggcac tgtagcagaa acagacgtca ctggcgagat ccaacgatat
    11521 tctcaacgcc ctatcacccg acacggcaat acaataaaaa ataacaataa ttgccggata
    11581 attgtactca attccgcctc tgttctcgcc aacgagtaac catataggca agtaagtaaa
    11641 acatgccaac tggcatcgcc aacctggaga agaaaatata aatatggatc attgtgctgc
    11701 cgctgtatcc cgacatcagc ccggaaatta ttgcgattgc tatcggttca ggtgcaattg
    11761 gctgcacgat cgttacggac tcgcttttct ggctagtgaa gcaatattgc ggcgctacgc
    11821 tcaatgaaac atttaaatac tatacgacag cgacatttat cgcttcagtc gtcgctctgg
    11881 cgggcacatt cctgctgtca tttatcatct aagcgcaaag agacgtacta tggaaaacgc
    11941 taaaatgaac tcgctcatcg cccagtatcc gttggtaaag gatctggttg ctcttaaaga
    12001 aaccacctgg tttaatcctg gcacgacctc attggctgaa ggtttacctt atgttggcct
    12061 gaccgaacag gatgttcagg acgcccatgc gcgcttatcc cgttttgcac cctatctggc
    12121 aaaagcattt cctgaaactg ctgccactgg ggggattatt gaatcagaac tggttgccat
    12181 tccagctatg caaaaacggc tggaaaaaga atatcagcaa ccgatcagcg ggcaactgtt
    12241 actgaaaaaa gatagccatt tgcccatttc cggctccata aaagcacgcg gcgggattta
    12301 tgaagtcctg gcacacgcag aaaaactggc tctggaagcg gggttgctga cgcttgatga
    12361 tgactacagc aaactgcttt ctccggagtt taaacagttc tttagccaat acagcattgc
    12421 tgtgggctca accggaaatc tggggttatc aatcggcatt atgagcgccc gcattggctt
    12481 taagatgaca gttcatatgt ctgctgatgc ccgggcatgg aaaaaagcga aactgcgcag
    12541 ccatggcgtt acggtcgtgg aatatgagca agattatggt gttgccgtcg aggaaggacg
    12601 taaagcagcg cagtctgacc cgaactgttt ctttattgat gacgaaaatt cccgcacgtt
    12661 gttccttggg tattccgtcg ctggccagcg tcttaaagcg caatttgccc agcaaggtcg
    12721 tatcgtcgat gctgataacc ctctgtttgt ctatctgccg tgtggtgttg gcggtggtcc
    12781 tggtggcgtc gcattcggac ttaaactggc gtttggcgat catgttcact gcttttttgc
    12841 cgaaccaacg cactcccctt gtatgttgtt aggcgtccat acaggattac acgatcagat
    12901 ttctgttcag gatattggta tcgacaacct taccgcagcg gatggccttg cagttggtcg
    12961 cgcatcaggc tttgtcgggc gggcaatgga gcgtctgctg gatggcttct atacccttag
    13021 cgatcaaacc atgtatgaca tgcttggctg gctggcgcag gaagaaggta ttcgtcttga
    13081 accttcagca ctggcgggta tggccggacc tcagcgcgtg tgtgcatcag taagttacca
    13141 acagatgcac ggtttcagcg cagaacaatt gcgtaatgcc actcatctgg tgtgggcgac
    13201 gggaggtgga atggtgccgg aagaagagat gaatcaatat ctggcaaaag gccgttaata
    13261 acgtttcaac gcagcatcgc aatcctttcc ctgggtgagc gatgctgccg atggcgcaga
    13321 cttaagatcc ccgctcttac ccgctataac cccctccttt tatcatcacc caacgccttt
    13381 cgctgtaaac ggcggtttcg caaaccaaac caaaaccgta agtaagataa aagcaatagc
    13441 cgccatacgg aaaatctcat ttgcagaaat agaaagtgac tgctgggtta tttcattatt
    13501 aatttcatta agaactcctg aaagcgaacc atagtattta tccataattt gtgacgatga
    13561 attaaacacg gggttaaatt gatcgatggt tgctgtcaac tgactatggt gtaacgattc
    13621 gcgtcgtccc cacagcgtca ttgtcaacga cgtaccaact gatcctgaca aggtacgaaa
    13681 aaaattactc atactcgagg cattggcaaa tttattatct ggcaagcctg aaaacgaaat
    13741 cgttgttaag ggtaaaaaga aacaggcaac ggcgaatccc tggaaaaact gtggcaaaat
    13801 gatgcctgta aaatcaatcg ttggcataaa tgtcacagaa cgccagtaat agcaaaccgc
    13861 atacatcaaa aaactaaatg tcactaacaa ccgcatgtct attttgttgc cataacgtcc
    13921 tatcaaaggt gaaattaata gtggcatgat gccgatgggc gcataagcaa gtccggccca
    13981 tatcgcatta taccccatcg tttcctggag taactgcggc ataaggacga tcgctccaga
    14041 gtaaaataaa tacgcgcatg tgatactcac aataccaatg gtgaagttac gggacttaaa
    14101 caaactgaga tcaagaatcg ggttctctga ggtcgactcc caaatgacta aagagatcag
    14161 agaaataact gatactactg ttaatattat tattgtactc gagttgaacc aatccagatc
    14221 gcgcccttta tcaagcataa tttgcaagcc accaacaccg agcactaaca gggtcagtcc
    14281 tggtagattc attttgaccg gtgaagtttc agtttctctt cctttaagta aggttaagca
    14341 taatgtcagg acgataatcc ccatagggac attgattaaa aatatccaac cccagctaaa
    14401 gttatcgcaa atataaccgc ccaatatcgg cccacatatc ggagcgataa tcacggtcat
    14461 tgaccataat gccagagcaa atgttctttt ttctggtgga taattcctta ataataaact
    14521 ctgtgacagt ggaattaacg gccccgccat taatccctga acgactctaa aaaatatcag
    14581 cacatcaaga ttggtcgata aactacacat taaggaagac agcgaaaaaa aagtgactga
    14641 aagtaaaaat aaccttaatt cgcctattct ttgtgccagc ctgccggtaa cagggatcgc
    14701 aatggcattt gctacgccaa acgaggtgat aacccaggtg ccttcgtctg ttgatgctcc
    14761 cagaaagcca gatattgtcg gtattgcgac gttagaaata gtggaatcca acatttgcat
    14821 aaacgtcgct aatgacaatg caatagtaac gcaccataac gtcccaccgg ttaatggtgc
    14881 cggagtcgat ttagtgattg ccattaaagt tgtccattat gcgaaataat gttgctaatt
    14941 tctttttcta tcggactggt atcgataact aaagccttac tggtataagc cggcatggac
    15001 gtcacggttg aagccagatc aggcatctca gcaatgtctt cgttcttcgt atcaatagtt
    15061 gcagtcatcg ataaaccaat gcgtaagggg tgttccatga gttcttttgg atcaagagaa
    15121 acttcaaccg gtacacgctg aacgattttg atccagttcc ctgtcgcatt ttgtgcaggt
    15181 aataaggaga acgcattgcc ggttcccata ttgatccctg tcacccgacc atgaaacaca
    15241 acattttcac cataaagatc gctgataata ttgactgatt gaccaatccg tacatccgtg
    15301 agttgtgttt ctttaaagtt ggcattaacc cacatttgac gtgccggtac gacagccatt
    15361 aacgattgtc cgggactcac tgtttcgccg acctgaacac tccgctgggc aatatagcca
    15421 gtaaccggac tccttatatc cgtacgttta agcgccaacc aggcttcttt agttgcatcc
    15481 gccgcttcca cgacttgtgg ctgacggttt aatggtgtgt tcattactaa agctttattc
    15541 gctttataag cctggatagc ggcattcagt gccgctttgc tacttattaa cgtatctttg
    15601 gtatgctcca gcgtttcttt tgaaataacc ccctgcttcg ctaacggtac tcgacggtta
    15661 taatcttcta aagattgttg atactgaata cgtgctgaag cgacttcggc actgtattgt
    15721 ttatcctgta agtacagttt attcgtttgc cgaacaatat ttgccagatt atttttagct
    15781 ttattgagtg cgatagtggc atcagtttta tccagtgaaa ctaaaatgtc accttgtcga
    15841 acgtagttcg tatctttatg attaacgaca gtgacactac ctgagacttg tgcagaaatt
    15901 ggatctgcat tccccgtgac ataggcgtca tctgtactaa tcatgtcttc taattccatt
    15961 gac
//
LOCUS       DAZKHF020000090        15810 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2201S31241
            SAMN43267800-rid25106413.denovo.091, whole genome shotgun sequence.
ACCESSION   DAZKHF020000090 DAZKHF020000000
VERSION     DAZKHF020000090.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267800
            Sequence Read Archive: SRR30307263
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 15810)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15810)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 15810)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350581.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350581.2
            Genome Coverage       :: 151x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:21:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,652
            CDSs (total)                      :: 5,557
            Genes (coding)                    :: 5,320
            CDSs (with protein)               :: 5,320
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 237
            CDSs (without protein)            :: 237
            Pseudo Genes (ambiguous residues) :: 0 of 237
            Pseudo Genes (frameshifted)       :: 102 of 237
            Pseudo Genes (incomplete)         :: 130 of 237
            Pseudo Genes (internal stop)      :: 52 of 237
            Pseudo Genes (multiple problems)  :: 41 of 237
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15810
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267800-rid25106413.denovo.091"
                     /serotype="O 26:H11"
                     /isolate="2201S31241"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-01"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            90..593
                     /locus_tag="ACHOSC_RS11890"
                     /old_locus_tag="pgaptmp_002378"
     CDS             90..593
                     /locus_tag="ACHOSC_RS11890"
                     /old_locus_tag="pgaptmp_002378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfbM family protein"
                     /protein_id="HGU4548305.1"
                     /translation="MGMIGYFAEIDSEKINQLLESTEKPLMDNIHDTLSGLRRLDIDK
                     RWDFLHFGLTGTSAFDPAKNDPLSRAVLGEHSLEDGIDGFLGLTWNQELAATIDRLES
                     LDRSELRKQFSIKRLNEMEIYPGVTFSEELEGQLFASIMLDMEKLISAYRRMLRQGNH
                     ALTVIVG"
     gene            complement(660..2117)
                     /gene="nuoN"
                     /locus_tag="ACHOSC_RS11895"
                     /old_locus_tag="pgaptmp_002379"
     CDS             complement(660..2117)
                     /gene="nuoN"
                     /locus_tag="ACHOSC_RS11895"
                     /old_locus_tag="pgaptmp_002379"
                     /EC_number="7.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416779.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoN"
                     /protein_id="HGU4548306.1"
                     /translation="MTITPQNLIALLPLLIVGLTVVVVMLSIAWRRNHFLNATLSVIG
                     LNAALVSLWFVGQAGAMDVTPLMRVDGFAMLYTGLVLLASLATCTFAYPWLEGYNDNK
                     DEFYLLVLIAALGGILLANANHLASLFLGIELISLPLFGLVGYAFRQKRSLEASIKYT
                     ILSAAASSFLLFGMALVYAQSGDLSFVALGKNLGDGMLNEPLLLAGFGLMIVGLGFKL
                     SLVPFHLWTPDVYQGAPAPVSTFLATASKIAIFGVVMRLFLYAPVGDSEAIRVVLAII
                     AFASIIFGNLMALSQTNIKRLLGYSSISHLGYLLVALIALQTGEMSMEAVGVYLAGYL
                     FSSLGAFGVVSLMSSPYRGPDADSLFSYRGLFWHRPILAAVMTVMMLSLAGIPMTLGF
                     IGKFYVLAVGVQAHLWWLVGAVVVGSAIGLYYYLRVAVSLYLHAPEQPGRDAPSNWQY
                     SAGGIVVLISALLVLVLGVWPQPLISIVRLAMPLM"
     gene            complement(2124..3653)
                     /gene="nuoM"
                     /locus_tag="ACHOSC_RS11900"
                     /old_locus_tag="pgaptmp_002380"
     CDS             complement(2124..3653)
                     /gene="nuoM"
                     /locus_tag="ACHOSC_RS11900"
                     /old_locus_tag="pgaptmp_002380"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_process: GO:0042773 - ATP synthesis coupled electron
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit M"
                     /protein_id="HGU4548307.1"
                     /translation="MLLPWLILIPFIGGFLCWQTERFGVKVPRWIALITMGLTLALSL
                     QLWLQGGYSLTQSAGIPQWQSEFDMPWIPRFGISIHLAIDGLSLLMVVLTGLLGVLAV
                     LCSWKEIEKYQGFFHLNLMWILGGVIGVFLAIDMFLFFFFWEMMLVPMYFLIALWGHK
                     ASDGKTRITAATKFFIYTQASGLVMLIAILALVFVHYNATGVWTFNYEELLNTPMSSG
                     VEYLLMLGFFIAFAVKMPVVPLHGWLPDAHSQAPTAGSVDLAGILLKTAAYGLLRFSL
                     PLFPNASAEFAPIAMWLGVIGIFYGAWMAFAQTDIKRLIAYTSVSHMGFVLIAIYTGS
                     QLAYQGAVIQMIAHGLSAAGLFILCGQLYERIHTRDMRMMGGLWSKMKWLPALSLFFA
                     VATLGMPGTGNFVGEFMILFGSFQVVPVITVISTFGLVFASVYSLAMLHRAYFGKAKS
                     QIASQELPGMSLRELFMILLLVVLLVLLGFYPQPILDTSRSAIGNIQQWFVNSVTTTR
                     P"
     gene            complement(3817..5658)
                     /gene="nuoL"
                     /locus_tag="ACHOSC_RS11905"
                     /old_locus_tag="pgaptmp_002381"
     CDS             complement(3817..5658)
                     /gene="nuoL"
                     /locus_tag="ACHOSC_RS11905"
                     /old_locus_tag="pgaptmp_002381"
                     /EC_number="7.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_process: GO:0042773 - ATP synthesis coupled electron
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit L"
                     /protein_id="HGU4548308.1"
                     /translation="MNMLALTIILPLIGFVLLAFSRGRWSENVSAIVGVGSVGLAALV
                     TAFIGVDFFANGEQSYSQPLWTWMSVGDFNIGFNLVLDGLSLTMLSVVTGVGFLIHMY
                     ASWYMRGEEGYSRFFAYTNLFIASMVVLVLADNLLLMYLGWEGVGLCSYLLIGFYYTD
                     PKNGAAAMKAFVVTRVGDVFLAFALFILYNELGTLNFREMVELAPAHFADGNNMLMWA
                     TLMLLGGAVGKSAQLPLQTWLADAMAGPTPVSALIHAATMVTAGVYLIARTHGLFLVT
                     PEVLHLVGIVGAVTLLLAGFAALVQTDIKRVLAYSTMSQIGYMFLALGVQAWDAAIFH
                     LMTHAFFKALLFLASGSVILACHHEQNIFKMGGLRKSIPLVYLCFLVGGAALSALPLV
                     TAGFFSKDEILAGAMANGHINLMVAGLVGAFMTSLYTFRMIFIVFHGKEQIHAHAVKG
                     VTHSLPLIVLLILSTFVGALIVPPLQGVLPQTTELAHGSMLTLEITSGVVAVVGILLA
                     AWLWLGKRTLVTSIANSAPGRLLGTWWYNAWGFDWLYDKVFVKPFLGIAWLLKRDPLN
                     SMMNIPAVLSRFAGKGLLLSENGYLRWYVASMSIGAVVVLALLMVLR"
     gene            complement(5655..5957)
                     /gene="nuoK"
                     /locus_tag="ACHOSC_RS11910"
                     /old_locus_tag="pgaptmp_002382"
     CDS             complement(5655..5957)
                     /gene="nuoK"
                     /locus_tag="ACHOSC_RS11910"
                     /old_locus_tag="pgaptmp_002382"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006683663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA];
                     GO_process: GO:0042773 - ATP synthesis coupled electron
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoK"
                     /protein_id="HGU4548309.1"
                     /translation="MIPLQHGLILAAILFVLGLTGLVIRRNLLFMLIGLEIMINASAL
                     AFVVAGSYWGQTDGQVMYILAISLAAAEASIGLALLLQLHRRRQNLNIDSVSEMRG"
     gene            complement(5954..6508)
                     /gene="nuoJ"
                     /locus_tag="ACHOSC_RS11915"
                     /old_locus_tag="pgaptmp_002383"
     CDS             complement(5954..6508)
                     /gene="nuoJ"
                     /locus_tag="ACHOSC_RS11915"
                     /old_locus_tag="pgaptmp_002383"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001774950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit J"
                     /protein_id="HGU4548310.1"
                     /translation="MEFAFYICGLIAILATLRVITHTNPVHALLYLIISLLAISGVFF
                     SLGAYFAGALEIIVYAGAIMVLFVFVVMMLNLGGSEIEQERQWLKPQVWIGPAILSAI
                     MLVVIVYAILGVNDQGIDGTPISAKAVGITLFGPYVLAVELASMLLLAGLVVAFHVGR
                     EERAGEVLSNRKDDSAKRKTEEHA"
     gene            complement(6520..7062)
                     /gene="nuoI"
                     /locus_tag="ACHOSC_RS11920"
                     /old_locus_tag="pgaptmp_002384"
     CDS             complement(6520..7062)
                     /gene="nuoI"
                     /locus_tag="ACHOSC_RS11920"
                     /old_locus_tag="pgaptmp_002384"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015959823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoI"
                     /protein_id="HGU4548311.1"
                     /translation="MTLKELLVGFGTQVRSIWMIGLHAFAKRETRMYPEEPVYLPPRY
                     RGRIVLTRDPDGEERCVACNLCAVACPVGCISLQKAETKDGRWYPEFFRINFSRCIFC
                     GLCEEACPTTAIQLTPDFEMGEYKRQDLVYEKEDLLISGPGKYPEYNFYRMAGMAIDG
                     KDKGEAENEAKPIDVKSLLP"
     gene            complement(7077..8054)
                     /gene="nuoH"
                     /locus_tag="ACHOSC_RS11925"
                     /old_locus_tag="pgaptmp_002385"
     CDS             complement(7077..8054)
                     /gene="nuoH"
                     /locus_tag="ACHOSC_RS11925"
                     /old_locus_tag="pgaptmp_002385"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015875065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoH"
                     /protein_id="HGU4548312.1"
                     /translation="MSWISPELIEILLTILKAVVILLVVVTCGAFMSFGERRLLGLFQ
                     NRYGPNRVGWGGSLQLVADMIKMFFKEDWIPKFSDRVIFTLAPMIAFTSLLLAFAIVP
                     VSPGWVVADLNIGILFFLMMAGLAVYAVLFAGWSSNNKYSLLGAMRASAQTLSYEVFL
                     GLSLMGVVAQAGSFNMTDIVNSQAHVWNVIPQFFGFITFAIAGVAVCHRHPFDQPEAE
                     QELADGYHIEYSGMKFGLFFVGEYIGIVTISALMVTLFFGGWQGPLLPPFIWFALKTA
                     FFMMMFILIRASLPRPRYDQVMSFGWKICLPLTLINLLVTAAVILWQAQ"
     gene            complement(8051..10777)
                     /gene="nuoG"
                     /locus_tag="ACHOSC_RS11930"
                     /old_locus_tag="pgaptmp_002386"
     CDS             complement(8051..10777)
                     /gene="nuoG"
                     /locus_tag="ACHOSC_RS11930"
                     /old_locus_tag="pgaptmp_002386"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311194.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050136 - NADH:ubiquinone reductase
                     (non-electrogenic) activity [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006120 - mitochondrial electron transport,
                     NADH to ubiquinone [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoG"
                     /protein_id="HGU4548313.1"
                     /translation="MATIHVDGKEYEVNGADNLLEACLSLGLDIPYFCWHPALGSVGA
                     CRQCAVKQYQNAEDTRGRLVMSCMTPASDGTFISIDDEEAKQFRESVVEWLMTNHPHD
                     CPVCEEGGNCHLQDMTVMTGHSFRRYRFTKRTHRNQDLGPFISHEMNRCIACYRCVRY
                     YKDYADGTDLGVYGAHDNVYFGRPEDGTLESEFSGNLVEICPTGVFTDKTHSERYNRK
                     WDMQFAPSICQQCSIGCNISPGERYGELRRIENRYNGTVNHYFLCDRGRFGYGYVNLK
                     DRPRQPVQRRGDDFITLNAEQAMQGAADILRQSKKVIGIGSPRASVESNFALRELVGE
                     ENFYTGIAHGEQERLQLALKVLREGGIYTPALREIESYDAVLVLGEDVTQTGARVALA
                     VRQAVKGKAREMAAAQKVADWQIAAILNIGQRAKHPLFVTNVDDTRLDDIAAWTYRAP
                     VEDQARLGFAIAHALDNSAPAVDGIEPELQSKIDVIVQALAGAKKPLIISGTNAGSIE
                     VIQAAANVAKALKGRGADVGITMIARSVNSMGLGIMGGGSLEEALTELETGRADAVVV
                     LENDLHRHASATRVNAALAKAPLVMVVDHQRTAIMENAHLVLSAASFAESDGTVINNE
                     GRAQRFFQVYDPAYYDSKTVMLESWRWLHSLHSTLLSREVDWTQLDHVIDAVVAKIPE
                     LAGIKDAAPDATFRIRGQKLAREPHRYSGRTAMRANISVHEPRQPQDIDTMFTFSMEG
                     NNQPTAHRSQVPFAWAPGWNSPQAWNKFQDEVGGKLRFGDPGVRLFETSENGLDYFTS
                     VPARFQPQDGKWRIAPYYHLFGSDELSQRAPVFQSRMPQPYIKLNPADAAKLGVNAGA
                     RVSFSYDGNTVTLPVEIAEGLTAGQVGLPMGMSGIAPVLAGAHLEDLKEAQQ"
     gene            complement(10830..12167)
                     /gene="nuoF"
                     /locus_tag="ACHOSC_RS11935"
                     /old_locus_tag="pgaptmp_002387"
     CDS             complement(10830..12167)
                     /gene="nuoF"
                     /locus_tag="ACHOSC_RS11935"
                     /old_locus_tag="pgaptmp_002387"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoF"
                     /protein_id="HGU4548314.1"
                     /translation="MKNIIRTPETHPLTWRLRDDKQPVWLDEYRSKNGYEGARKALTG
                     LSPDEIVNQVKDAGLKGRGGAGFSTGLKWSLMPKDESMNIRYLLCNADEMEPGTYKDR
                     LLMEQLPHLLVEGMLISAFALKAYRGYIFLRGEYIEAAVNLRRAIAEATEAGLLGKNI
                     MGTGFDFELFVHTGAGRYICGEETALINSLEGRRANPRSKPPFPATSGAWGKPTCVNN
                     VETLCNVPAILANGVEWYQNISKSKDAGTKLMGFSGRVKNPGLWELPFGTTAREILED
                     YAGGMRDGLKFKAWQPGGAGTDFLTEAHLDLPMEFESIGKAGSRLGTALAMAVDHEIN
                     MVSLVRNLEEFFARESCGWCTPCRDGLPWSVKILRALERGEGQPGDIETLEQLCRFLG
                     PGKTFCAHAPGAVEPLQSAIKYFREEFEAGIKQPFSNTHLINGIQPNLLKERW"
     gene            complement(12164..12664)
                     /gene="nuoE"
                     /locus_tag="ACHOSC_RS11940"
                     /old_locus_tag="pgaptmp_002388"
     CDS             complement(12164..12664)
                     /gene="nuoE"
                     /locus_tag="ACHOSC_RS11940"
                     /old_locus_tag="pgaptmp_002388"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoE"
                     /protein_id="HGU4548315.1"
                     /translation="MHENQQPQTEAFELSAAEREAIEHEMHHYEDPRAASIEALKIVQ
                     KQRGWVPDGAIHAIADVLGIPASDVEGVATFYSQIFRQPVGRHVIRYCDSVVCHINGY
                     QGIQAALEKKLNIKPGQTTFDGRFTLLPTCCLGNCDKGPNMMIDEDTHAHLTPEAIPE
                     LLERYK"
     gene            complement(12667..14469)
                     /gene="nuoC"
                     /locus_tag="ACHOSC_RS11945"
                     /old_locus_tag="pgaptmp_002389"
     CDS             complement(12667..14469)
                     /gene="nuoC"
                     /locus_tag="ACHOSC_RS11945"
                     /old_locus_tag="pgaptmp_002389"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030964 - NADH dehydrogenase complex
                     [Evidence IEA];
                     GO_function: GO:0048038 - quinone binding [Evidence IEA];
                     GO_function: GO:0050136 - NADH:ubiquinone reductase
                     (non-electrogenic) activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit C/D"
                     /protein_id="HGU4548316.1"
                     /translation="MVNNMTDLTAQEPAWQTRDHLDDPVIGELRNRFGPDAFTVQATR
                     TGVPVVWIKREQLLEVGDFLKKLPKPYVMLFDLHGMDERLRTHREGLPAADFSVFYHL
                     ISIDRNRDIMLKVALAENDLHVPTFTKLFPNANWYERETWDLFGITFDGHPNLRRIMM
                     PQTWKGHPLRKDYPARATEFSPFELTKAKQDLEMEALTFKPEEWGMKRGTENEDFMFL
                     NLGPNHPSAHGAFRIVLQLDGEEIVDCVPDIGYHHRGAEKMGERQSWHSYIPYTDRIE
                     YLGGCVNEMPYVLAVEKLAGITVPDRVNVIRVMLSELFRINSHLLYISTFIQDVGAMT
                     PVFFAFTDRQKIYDLVEAITGFRMHPAWFRIGGVAHDLPRGWDRLLREFLDWMPKRLA
                     SYEKAALQNTILKGRSQGVAAYGAKEALEWGTTGAGLRATGIDFDVRKARPYSGYENF
                     DFEIPVGGGVSDCYTRVMLKVEELRQSLRILEQCLNNMPEGPFKADHPLTTPPPKERT
                     LQHIETLITHFLQVSWGPVMPANESFQMIEATKGINSYYLTSDGSTMSYRTRIRTPSY
                     AHLQQIPAAIRGSLVSDLIVYLGSIDFVMSDVDR"
     gene            complement(14563..15225)
                     /gene="nuoB"
                     /locus_tag="ACHOSC_RS11950"
                     /old_locus_tag="pgaptmp_002390"
     CDS             complement(14563..15225)
                     /gene="nuoB"
                     /locus_tag="ACHOSC_RS11950"
                     /old_locus_tag="pgaptmp_002390"
                     /EC_number="7.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoB"
                     /protein_id="HGU4548317.1"
                     /translation="MDYTLTRIDPNGENDRYPLQKQEIVTDPLEQEVNKNVFMGKLND
                     MVNWGRKNSIWPYNFGLSCCYVEMVTSFTAVHDVARFGAEVLRASPRQADLMVVAGTC
                     FTKMAPVIQRLYDQMLEPKWVISMGACANSGGMYDIYSVVQGVDKFIPVDVYIPGCPP
                     RPEAYMQALMLLQESIGKERRPLSWVVGDQGVYRANMQSERERKRGERIAVTNLRTPD
                     EI"
     gene            complement(15241..15684)
                     /gene="nuoA"
                     /locus_tag="ACHOSC_RS11955"
                     /old_locus_tag="pgaptmp_002391"
     CDS             complement(15241..15684)
                     /gene="nuoA"
                     /locus_tag="ACHOSC_RS11955"
                     /old_locus_tag="pgaptmp_002391"
                     /EC_number="7.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoA"
                     /protein_id="HGU4548318.1"
                     /translation="MSMSTSTEVIAHHWAFAIFLIVAIGLCCLMLVGGWFLGGRARAR
                     SKNVPFESGIDSVGSARLRLSAKFYLVAMFFVIFDVEALYLFAWSTSIRESGWVGFVE
                     AAIFIFVLLAGLVYLVRIGALDWTPARSRRERMNPETNSIANRQR"
BASE COUNT         3892 a         4507 c         4188 g         3223 t
ORIGIN      
        1 aggcgggtcc agaaaaagcg acatgcacat tttattttcc ctcgtctgtt ttaagatgcg
       61 aaacatgaaa ttcaatggag gaaaaataaa tgggaatgat tggttatttt gcagaaattg
      121 actcagaaaa gattaaccaa ctgcttgagt ctactgaaaa acctttgatg gataatatac
      181 atgacacgct ttcaggatta cgccgactgg atattgataa acgctgggat tttttacact
      241 ttgggctgac aggcacctct gcttttgatc ccgcgaagaa tgatccgctg agtcgtgccg
      301 tgcttggtga acacagcctc gaagatggca tagatggctt cctgggattg acctggaatc
      361 aggagttagc cgcgacaatc gaccgactgg aatcactgga tcgaagtgag ctgcgtaaac
      421 agttttccat taagcgactc aatgaaatgg agatttatcc gggagtgacc ttttcagaag
      481 agttagaggg gcaactcttt gcttcgatca tgcttgatat ggaaaagctg attagcgcgt
      541 atcgacgtat gctgcgacaa gggaatcatg cgctgacggt tattgttggg tgatggtgtc
      601 agggcaccaa aaaaaaccgc cgaacatgtc ggcggctttc tgacttacaa actaacagat
      661 tacatcagcg gcattgctaa ccgcacaatg ctaatcagcg gttgtggcca tacacccagc
      721 accagtacca acagtgcaga aatcagcacc acgataccgc ccgcgctgta ctgccagttt
      781 gatggtgcat cgcgacccgg ttgttccggg gcgtgaagat acaggctcac cgccacgcgc
      841 aggtagtagt agaggccgat tgccgaaccg acaaccacgg cacccaccag ccaccacaag
      901 tgtgcctgga caccgaccgc cagcacgtag aacttaccga taaagcccag cgtcatcggg
      961 ataccggcca gagacagcat catcaccgtc atcactgccg cgaggatcgg acgatgccag
     1021 aacagaccgc ggtaagagaa cagggaatca gcatccgggc cacgatacgg gctggacatc
     1081 aggctgacca cgccgaacgc gccgaggctg ctgaacagat aaccggccag gtaaacccct
     1141 accgcttcca tcgacatctc accagtttgc agcgcaatca gcgccaccag cagatagccg
     1201 aggtgagaga tagatgagta accgagcaga cgtttgatgt tggtctggct cagcgccatc
     1261 aggttaccga agatgatgga ggcaaaggcg ataatcgcca gtactacgcg aatcgcttcg
     1321 ctgtcaccca ccggtgcgta gaggaacaga cgcatcacca caccgaagat agcgattttg
     1381 ctcgccgtcg ccaggaaagt ggaaaccggc gcaggcgcgc cctggtatac gtctggcgtc
     1441 cacaggtgga acggcaccag cgagagtttg aagccgaggc caacaatcat cagtccgaaa
     1501 cctgccagca acagcggctc gttgagcata ccgtcgccga ggtttttacc cagcgcgaca
     1561 aacgacaggt cgccagactg tgcatacacc agcgccatac caaacagcag gaaagaagac
     1621 gctgcggcag aaaggatggt gtatttgata ctggcttcca gcgaacgttt ctggcggaaa
     1681 gcgtaaccga ccaggccaaa cagcggcaaa gagatcagtt caataccgag gaacaaagac
     1741 gccagatggt tggcattcgc cagcaggatc ccgcccagcg cggcaattaa caccaacagg
     1801 tagaactcat ccttgttgtc gttatagcct tcaagccacg ggtaggcgaa agtacaggtg
     1861 gcgaggctcg ccaacaatac cagcccggtg taaagcatgg cgaaaccatc aacgcgcatc
     1921 agcggcgtaa cgtccatagc gcccgcctgg ccgacaaacc agagcgaaac cagcgccgcg
     1981 ttaagcccaa taaccgagag cgtagcgttg aggaaatgat tgcgtcgcca cgcaatggag
     2041 agcatcacaa ccaccaccgt caagccgacg atcagcaacg gtagcagtgc gatcaggttt
     2101 tgtggagtta ttgtcatggc gatttacggc cttgtagtag taacggaatt aacaaaccac
     2161 tgctggatat tgccaatcgc ggagcgcgag gtatccagaa tcggctgcgg atagaagccc
     2221 agcagtacca gcagcaccac cagcaacagg atcataaaca gctcacgcag cgacatccct
     2281 ggcagttcct ggctggcaat ctggcttttc gctttaccga agtaagcgcg atgcaacatc
     2341 gccagcgaat aaacagatgc aaagaccagc ccaaaggtag agataacggt aatcaccggg
     2401 acaacctgga agctgccgaa cagaatcata aattcgccga cgaagttacc ggtgccaggc
     2461 atcccaagcg ttgccaccgc aaagaacagc gacagtgctg gcagccattt catcttgctc
     2521 cacagaccgc ccatcatgcg catgtcgcgg gtatggatac gttcataaag ctgaccacaa
     2581 agaataaaca gacccgccgc cgacaagccg tgcgcaatca tctggattac cgcgccctgg
     2641 taggccaact ggctgccggt gtagatagca atcagcacga agcccatgtg ggaaaccgag
     2701 gtgtaggcga tcagacgttt gatatcggtc tgggcgaagg ccatccacgc accgtagaag
     2761 atgccgataa cacccagcca catagcaatt ggcgcgaact ctgccgacgc gttcgggaac
     2821 agcggcaggg agaaacgcag caaaccgtaa gcggcagttt tcagcaagat tcccgcgagg
     2881 tcaacggaac cggcggtcgg agcctgggag tgcgcatccg gcagccagcc atgcagcgga
     2941 accaccggca ttttgactgc gaaggcgatg aagaagccca gcattaacag gtattccaca
     3001 ccactggaca ttggcgtatt cagcagctct tcatagttga aggtccagac gccggtcgca
     3061 ttgtagtgaa caaaaaccag cgccaggatg gcaatcaaca tcaccaggcc actcgcctgg
     3121 gtgtaaatga agaacttggt tgccgccgtg atacgcgttt taccgtcaga ggctttatgc
     3181 ccccacagtg cgatcaggaa gtacatcggc accagcatca tttcccagaa gaagaagaac
     3241 aggaacatgt cgatggcaag gaacacgccg ataacgccgc ccaggatcca catcaggttg
     3301 aggtggaaga agccctgata tttttcgatc tctttccacg aacatagtac cgccagcaca
     3361 ccgagcagac cggtcagcac gaccatcagc agcgacagcc cgtcaatggc gagatgaata
     3421 gagataccaa aacgcgggat ccacggcatg tcgaattcag actgccactg cggaattccg
     3481 gcggattgcg tcagtgaata accgccctgc aaccacagtt gcagcgacag cgccagcgtc
     3541 aatcccatgg tgatcagcgc gatccagcgt ggcaccttga cgccaaagcg ttcggtctgc
     3601 cagcacagga agccgccaat aaagggaatt aatattagcc agggtagtaa catggcgatc
     3661 tttattcctt gtaaaagtcc cgtcaggacc ggattttcaa cgaattctca cgacagaaaa
     3721 ttctcttcgg actgggggta gtgtcggatg caccgcgaag gcggcttatc cgacctacgt
     3781 tcttatatgc ccaggggcaa tcccacaatc cttaactcaa cgcagtacca tcaacagtgc
     3841 cagcaccacg accgcaccga tgctcatgga tgccacatac cagcgcagat agccgttctc
     3901 acttaacagc agacctttac ctgcaaagcg ggaaaggaca gccgggatgt tcatcattga
     3961 gttcagcgga tcgcgtttca gcaaccaggc aatacccagg aacggcttga cgaacacttt
     4021 gtcatacagc cagtcaaatc cccaggcgtt gtaccaccag gtgcccagca gacggcccgg
     4081 cgcactgttg gcgatggagg tcaccagagt acgtttaccc agccacagcc aggctgccag
     4141 cagaatgccg accaccgcga ccacgccaga ggtaatttcc agggtcaaca tgctgccgtg
     4201 cgccagttcc gtcgtttgcg gaagcacgcc ctgcagcggc ggtacaatca gtgcgccaac
     4261 gaaggtggaa aggatcagca gcacaatcag cggcaggctg tgagttaccc ctttcacggc
     4321 gtgagcgtga atttgttctt ttccgtggaa gacgatgaaa atcatacgga aggtgtagag
     4381 cgaggtcata aacgcaccga ccagacctgc caccatcaga ttgatatgac cattcgccat
     4441 cgcacccgcg aggatctcat ccttactgaa gaagcccgca gtgaccagcg gtagtgccga
     4501 cagtgctgcg ccgcccacca ggaagcagag ataaaccagc ggaatagatt tacgcagacc
     4561 gcccatcttg aagatgttct gttcgtgatg gcaggccaga atgacggaac cggatgccag
     4621 gaacagcagc gctttaaaga acgcgtgagt catcaagtgg aaaatcgccg catcccatgc
     4681 ctgcacgcca agcgcgagga acatgtagcc aatctggctc atggtagagt aagcgagaac
     4741 acgtttgatg tcggtctgta ccagcgcggc aaaaccggcc agcagcagcg taaccgcccc
     4801 gacaataccc accagatgca gaacttccgg cgtcaccagg aacaggccgt gggtacgggc
     4861 gatcaggtag acacccgcgg ttaccatcgt tgcggcgtgg atcagcgcgg agacaggcgt
     4921 cgggcccgcc atcgcgtcag caagccatgt ctgcaacggc aactgcgcag atttaccgac
     4981 cgcaccgccc agcagcatca gcgtcgccca catcagcatg ttattgccgt cagcaaagtg
     5041 cgctggtgcc agttccacca tttcgcggaa gttcagggtg cccagttcgt tgtaaagaat
     5101 gaacagtgcg aaagcgagga aaacgtcacc cacacgggtc acgacgaacg ctttcattgc
     5161 cgctgcgcca ttcttcggat cggtgtaata gaaaccgatc agcagatagg agcacaggcc
     5221 cacgccttcc cagccgaggt acatcagcag caggttatcg gcgagaacca gaactaccat
     5281 gctggcgatg aacaggttgg tgtaggcgaa gaagcgagag tagccctctt caccgcgcat
     5341 gtaccaggag gcgtacatgt gaataaggaa acccacacca gtgaccaccg agagcatggt
     5401 cagcgacagg ccgtccagca ccaggttaaa accgatgtta aagtcgccta ccgacatcca
     5461 cgtccacagc ggctggctgt atgactgctc gccgttagcg aagaaatcaa cgccgataaa
     5521 ggcggttacc agcgccgcca ggcccacaga gccaacgccg acgatcgccg aaacgttttc
     5581 agaccagcgc ccacgggaga acgccagcag gacgaagcca atcaatggca aaataatggt
     5641 taaggcaagc atgttcatcc gcgcatctca cttactgaat cgatgttcag gttctggcga
     5701 cgacggtgaa gttgcagcag cagcgcaagg ccgatactcg cttctgccgc cgcgaggctg
     5761 atggcgagaa tgtacatcac ctgaccgtcg gtctgacccc agtagcttcc ggcgaccaca
     5821 aaggccagcg cggaggcgtt aatcatgatt tccagaccaa tcagcataaa cagcagattg
     5881 cgacggataa ccagaccggt taagccaaga acgaataaga ttgccgcgag gatcagtcca
     5941 tgttgtaagg ggatcatgcg tgctcctccg tttttctttt cgcgctgtcg tctttacgat
     6001 tgctcagcac ttcacccgca cgctcttcac gaccgacgtg gaaggccaca accagacccg
     6061 cgagcagcag catagaagcc agttccaccg ccagtacgta aggcccgaac agcgtaatac
     6121 ccactgcttt agcactgatt ggcgtaccgt cgataccctg atcattaaca ccaaggatgg
     6181 cgtaaacaat caccaccagc atgatggccg acaaaattgc cggaccaatc cacacctgcg
     6241 gtttcagcca ctggcgttcc tgttcgattt ctgaaccgcc caggttgagc atcatcacca
     6301 cgaacacgaa cagcaccata atggcacccg cgtagacgat aatttccagc gcaccggcga
     6361 agtaagcgcc cagtgagaag aacaccccgg aaatcgccag cagcgaaata atcaggtaca
     6421 gcagtgcgtg taccggattg gtatgggtga tcactcgcaa ggtcgcaagt atggctatca
     6481 ggccacagat ataaaaagcg aactccattg cccctctcct tacggtaaca ggctcttgac
     6541 gtcgataggc ttggcttcgt tctctgcttc gcccttatct ttgccgtcga ttgccatacc
     6601 tgccatccgg tagaagttat attccgggta tttgcccgga ccggagatca gcagatcctc
     6661 tttttcgtaa accagatcct ggcgcttgta ttcccccatt tcgaaatccg gggttaactg
     6721 aatcgccgtg gtcggacagg cttcttcgca cagaccacag aaaatgcagc gtgagaagtt
     6781 gatgcggaaa aattccgggt accagcgacc gtctttggtt tctgcttttt gcagcgagat
     6841 acagccgacc gggcaggcta ccgcgcagag gttacaggct acgcaacgct cttcgccgtc
     6901 cgggtcgcgg gtcagaacga tacgaccacg ataacggggc ggcagataga ccggctcttc
     6961 cgggtacatt cgcgtttcgc gtttggcgaa cgcgtgcagg ccgatcatcc agatactacg
     7021 aacctgggtg ccgaaaccta ctaacaattc ttttaaggtc atggtcttat tgccccttat
     7081 tgcgcctgcc agagaatgac agccgccgtt accagcaagt tgatcagcgt cagcggcagg
     7141 cagattttcc agccgaagga cattacctgg tcataacgcg gacgcggtaa cgacgcacga
     7201 atcaaaatga acatcatcat aaagaacgcg gttttcagcg cgaaccagat gaatggcggt
     7261 aacaacgggc cttgccagcc accgaagaac agcgtcacca tcaatgcaga gatggtcaca
     7321 atcccgatgt actcacccac gaagaacaga ccgaacttca taccggaata ttcaatgtgg
     7381 taaccatccg ccagttcctg ctcggcttcc ggctggtcaa acgggtgacg gtgacatacc
     7441 gccacgcccg cgatggcaaa ggtaataaaa ccaaagaatt gcgggataac gttccacaca
     7501 tgcgcctggc tgttgacgat gtcggtcatg ttgaatgaac cggcctgcgc caccacgccc
     7561 atcagggaaa gcccgaggaa cacttcgtag ctcagggtct gcgcagaagc acgcatcgca
     7621 cccagcaacg agtatttgtt gttacttgac cagcccgcaa acagcaccgc gtaaaccgcc
     7681 agacctgcca tcatcaggaa gaacaaaatc ccgatgttca ggtcggcaac cacccaaccc
     7741 ggactgactg gcacaatcgc aaaggccagc agcagcgagg taaaggcaat catcggtgcc
     7801 agggtaaaga tgacgcgatc cgagaatttc gggatccagt cttctttaaa gaacattttg
     7861 atcatgtccg caaccagctg gagcgaaccg ccccagccaa cacggttagg tccgtaacgg
     7921 ttctggaaca gacccagcag gcgacgttcg ccaaagctca tgaatgcccc gcaggtgaca
     7981 accaccagca ggatcaccac cgctttgagg atggtcagca ggatctcaat cagttccggt
     8041 gatatccaac tcattgttgt gcctccttga gatcctcaag atgcgcgcca gccagcaccg
     8101 gagcaatgcc ggacataccc atcggcaaac ccacctgccc tgccgtcagc ccttcagcga
     8161 tttcaaccgg cagcgtgacc gtgttgccat cgtaactaaa ggagacgcga gcgcctgcgt
     8221 tcacgcccaa cttcgcggca tccgctgggt tgagtttgat gtacggctgc ggcatacggc
     8281 tctggaagac cggagcacgc tgtgacaatt catcgctgcc aaacaggtgg taatacggcg
     8341 cgatacgcca tttcccgtcc tgcggctgga agcgtgccgg tacgctggtg aagtaatcca
     8401 gaccattttc gctggtttca aacagacgca cgcccggatc gccaaagcgc agtttgccgc
     8461 ccacttcgtc ctggaatttg ttccacgcct gcggggagtt ccagcccggc gcccaggcaa
     8521 acggcacttg cgaacggtgc gcagtcggct ggttgttacc ttccatcgag aaggtgaaca
     8581 tggtgtcaat atcctgcggc tgacgcggct catgaacgct gatattggcg cgcatggcgg
     8641 tacgaccgct gtaacggtgc ggttcacggg ccagtttctg cccacgaata cggaatgtcg
     8701 catccggcgc agcatctttg atacctgcca gttccgggat tttcgccaca acagcgtcaa
     8761 tcacatggtc gagctgcgtc cagtccactt cacggctcag cagggtgctg tgcagcgagt
     8821 gtaaccagcg ccagctttcc agcatgacag ttttgctgtc gtaataagca ggatcgtaaa
     8881 cctggaagaa acgttgggcg cggccttcgt tgttgatcac cgtaccgtcg ctttcagcaa
     8941 agctggcagc agaaagtacc agatgggcgt tttccataat cgctgtgcgt tgatgatcaa
     9001 ccaccatcac cagcggcgct ttagccagcg cagcattcac gcgggtagcg gaagcgtgac
     9061 gatgcagatc gttttccagc accaccaccg cgtcggcgcg tccggtttcc agttcggtca
     9121 acgcttcttc aagcgaaccg ccacccataa tgcccagccc catgctgttg acggaacggg
     9181 caatcatggt gataccaacg tcagcgccgc gacctttcag ggctttcgcg acgttagccg
     9241 cagcctgaat cacctctatg ctaccggcgt tcgtcccgga gataatcaac ggtttcttcg
     9301 cacctgccag tgcctgcacg atgacgtcga ttttgctttg cagctcaggt tcgataccgt
     9361 caaccgctgg cgcagagtta tccagcgcat gggcgatggc aaaacctaaa cgcgcctgat
     9421 cttcaaccgg tgcgcggtaa gtccacgccg cgatatcatc cagacgggtg tcatcaacgt
     9481 tggtaacaaa cagcggatgc ttcgcacgtt gaccgatgtt gaggattgcc gcaatctgcc
     9541 agtcagccac tttctgtgct gccgccattt cgcgcgcttt acctttcaca gcctgacgca
     9601 ctgccagcgc gacgcgcgcg ccggtctggg taacgtcttc gcccagcacc agtaccgcat
     9661 cgtaagattc gatttcgcgc agagccggag tataaatgcc gccttcacgc agcactttca
     9721 gcgccagttg cagacgttcc tgctcaccgt gagcgatacc ggtgtagaag ttttcttcgc
     9781 ccaccagttc acgcagcgca aagttgcttt ccacgctggc acgcggagaa ccaataccga
     9841 tcactttctt cgactgacgc agaatatctg ccgcgccctg cattgcctgt tcggcgttga
     9901 gggtaatgaa atcatcgcca cgacgctgta ctggctgacg cggacgatcc ttcaggttga
     9961 cgtaaccgta accgaaacga ccacggtcgc agaggaagta gtggtttacc gtaccgttgt
    10021 aacggttttc gatacgacgc agttcgccgt aacgttcacc ggggctgatg ttacagccga
    10081 tggaacattg ctggcagatg ctcggcgcaa actgcatatc ccatttacgg ttgtaacgct
    10141 cggagtgcgt tttgtcggta aatacgccgg tcgggcaaat ttcgaccagg ttaccggaaa
    10201 attcgctttc cagcgtgccg tcttccgggc gaccgaagta gacgttgtcg tgcgcaccgt
    10261 aaacgcccag atctgtaccg tcagcgtaat ctttgtagta acgcacacag cggtagcagg
    10321 cgatgcagcg gttcatttcg tgagagatga atggccccaa atcctgatta cggtgggtac
    10381 gtttggtgaa acggtagcga cggaagctgt gtccggtcat cacggtcata tcctgaagat
    10441 ggcagttacc gccttcttca cataccggac agtcgtgcgg gtggttggtc attaaccact
    10501 cgaccacact ttcacggaac tgtttcgctt cttcgtcgtc aatggaaata aaggtgccat
    10561 cggaagccgg tgtcatacag gacatcacca ggcgaccacg cgtgtcttcc gcgttttggt
    10621 attgcttcac cgcacactgg cggcaagcac cgacgcttcc cagcgccgga tgccagcaaa
    10681 agtaaggaat atcaaggccc agagacagac aagcttccag caggttgtcc gctccgttga
    10741 cctcgtattc tttgccgtct acatgaattg tagccattag catgcttcca gttttctcag
    10801 tcagagactg agcgttaatc gaaattcggt taccagcgct ctttcagcag gttcggctga
    10861 atcccattaa tcaaatgggt attgctgaac ggctgtttga ttcccgcctc aaattcttcg
    10921 cggaaatatt tgatggcgct ctgtaacggc tccactgcac caggtgcgtg ggcacagaaa
    10981 gttttacccg ggcctaagaa tcgacacagt tgctcaagtg tttcgatatc gcccggctga
    11041 ccttcaccac gctccagcgc acgcagaatt ttcacgctcc acggcagacc gtcgcggcac
    11101 ggcgtacacc agccgcagga ttcacgggcg aaaaactctt ccaggttacg caccagcgac
    11161 accatgttga tctcatggtc aaccgccatc gccagcgccg tacccagacg gcttcctgct
    11221 ttaccgatac tttcgaattc catcggcagg tcaagatgcg cttcggtcag gaagtcggtg
    11281 cccgcgccgc ctggctgcca ggctttgaat ttcagaccgt cacgcatacc accggcgtaa
    11341 tcttcgagga tctcgcgcgc ggtagtacca aacggcagtt cccacaggcc cggatttttc
    11401 acccgaccgg agaagcccat cagcttggtg ccagcatctt tacttttcga gatgttctga
    11461 taccactcca cgccgttagc gaggatcgcc ggaacgttac acagggtttc gacgttgttg
    11521 acacaggtcg gtttacccca tgcgccggag gtcgccggga atggaggctt cgagcgtggg
    11581 ttagcacgac gtccttccag ggagttgatt aacgctgttt cttccccgca gatgtagcgc
    11641 cctgccccgg tatggacgaa cagttcgaag tcaaaacctg ttcccataat atttttgcca
    11701 agcagacctg cttcggtggc ttcggcaatg gcacggcgta gattcactgc cgcttcgata
    11761 tattcgccac gcaggaagat gtagccacgg taagctttca gcgcaaacgc ggagatgagc
    11821 ataccttcca ccagcaggtg cggcagttgc tccatcaaca ggcggtcttt ataggtgccc
    11881 ggctccattt catcggcgtt acacagcagg taacggatgt tcatggattc gtctttcggc
    11941 atcaggctcc acttcaggcc agtcgaaaag cccgcgccgc cgcgcccttt cagaccagcg
    12001 tcttttacct gattaacgat ttcgtccgga gacagcccgg tcagcgcctt acgcgcccct
    12061 tcgtaaccat ttttgctgcg gtattcgtcc agccacactg gctgtttgtc atcgcgcaga
    12121 cgccaggtca gcggatgcgt ttcgggagta cggataatgt ttttcattta taccgctcca
    12181 gcagttcagg gatcgcttcc ggggtcaaat gcgcgtgagt gtcctcatcg atcatcatat
    12241 ttggcccttt atcacagttc cccaggcagc aggttggcag cagcgtaaag cgaccgtcaa
    12301 acgtcgtttg ccctggtttg atgttcagtt ttttctccag cgccgcctga ataccctggt
    12361 aaccgttgat gtgacagacc acgctgtcgc aataacggat cacatggcga ccaaccggtt
    12421 ggcggaagat ctggctatag aacgttgcca cgccttcgac atcgctcgcc ggaataccca
    12481 gcacatcggc gatcgcgtgg atcgcgccgt ccggcaccca gccacgctgc ttctgaacga
    12541 ttttcagcgc ttcaatggac gccgcacgcg ggtcttcgta gtggtgcatc tcgtgctcaa
    12601 tcgcttcacg ctctgccgca ctcagctcaa aagcctcggt ttgtggttgt tgattctcgt
    12661 gcataattag cggtccacat ctgacataac aaaatcgata ctgcccagat aaacaatcag
    12721 gtcagacacc aggctgccgc ggatcgccgc cggaatttgc tgcaaatgcg cataactcgg
    12781 cgtgcggata cgagtgcgat aactcatggt gctaccgtcg ctggtcaggt agtaactgtt
    12841 gatccccttg gtcgcctcaa tcatctggaa agattcattg gcaggcatca ccggccccca
    12901 cgacacttgc aggaagtggg tgatcagggt ttcgatatgt tgcagcgtgc gctctttcgg
    12961 cggcggcgtg gtcagcgggt gatccgcttt gaacgggcct tccggcatgt tgttgaggca
    13021 ctgctcaaga atgcgcagac tctggcgcag ctcttccact ttcagcatta cgcgggtgta
    13081 gcagtcagaa acaccaccac caaccgggat ttcaaagtcg aagttttcat agccagaata
    13141 aggacgcgcc ttacgcacgt cgaagtcgat cccggtagca cgcaggcccg cgccagtggt
    13201 gccccactcc agcgcctctt tcgcgccata ggcggcaacg ccctgggaac gacctttcag
    13261 aatggtattt tgcagcgccg ctttctcgta agacgccaga cgtttcggca tccagtcgag
    13321 gaactcacgc agcaggcgat cccagccgcg cggcaggtcg tgcgctacgc cgccaatacg
    13381 gaaccacgcc gggtgcatac ggaaacccgt gatcgcttcc accagatcgt aaattttctg
    13441 acgatcggta aaggcgaaga acacgggcgt cattgcgccg acgtcctgaa taaaggtcga
    13501 gatgtacagc aggtggctgt tgatacggaa cagttcggag agcataacgc gaatgacgtt
    13561 aacgcgatcc ggcacggtga tcccggccag tttctctacc gccagcacgt aaggcatttc
    13621 gttaacgcag ccgccgaggt attcaatacg gtcggtatac ggaatgtagc tgtgccagga
    13681 ctggcgctcg cccattttct ccgcaccacg gtggtggtag ccgatgtctg gtacgcagtc
    13741 gacaatctct tcgccgtcga gttgtaaaac gatacggaaa gccccgtgcg ccgacgggtg
    13801 gttcggaccg aggttgagga acatgaagtc ctcgttttcg gtgccgcgct tcatccccca
    13861 ctcttccggt ttgaaggtca gggcttccat ctccagatcc tgtttggctt tggtcagctc
    13921 aaacggcgag aattcggtag cgcgcgccgg gtaatcttta cgcagcgggt gacctttcca
    13981 ggtttgcggc atcatgatgc ggcgcaggtt cgggtgaccg tcgaaagtaa tgccaaacag
    14041 atcccaggtt tcacgctcat accagttagc gttcgggaac agtttggtga aggtcggtac
    14101 gtgcaggtcg ttttctgcca gcgccacctt cagcatgatg tcgcggttac gatcgataga
    14161 aatcagatgg tagaaaacgg aaaaatccgc ggcaggtaac ccttcgcggt gtgtgcgcag
    14221 acgttcgtcc atgccgtgta agtcaaacag catgacgtaa ggtttcggca gtttctttaa
    14281 gaaatcgcca acttccagta attgttcacg cttgatccac acaacgggaa ccccggtgcg
    14341 agtcgcctga acagtaaagg catccggccc aaaacggttg cgcagttcgc caattaccgg
    14401 atcatcaaga tgatcgcggg tctgccaggc gggttcttgc gcggttaagt cggtcatatt
    14461 gttcaccatt gcaaatggtc cgtggtgact gtcggcgcag taagcttcgc gaaatagatt
    14521 taggaataag cgaagaaaaa tccctttgcc gacaggcgca aattaaatct cgtcaggtgt
    14581 acgcaggttg gttacggcaa tacgttcacc gcgcttgcgt tcgcgctctg attgcatatt
    14641 ggcgcgataa acgccctgat cgccaaccac ccaggagagc ggacgacgtt ctttgccgat
    14701 agattcctgc aacagcatca gtgcctgcat gtacgcttca ggacgcggcg ggcagcccgg
    14761 gatatacaca tcaaccggga tgaatttatc gacgccctgc acaacggaat aaatatcgta
    14821 cataccacca gagttggcac aggcacccat tgagataacc cattttggtt ccagcatctg
    14881 gtcatacaga cgctgaataa ccggtgccat tttggtaaag caggttcctg caaccaccat
    14941 caggtcagcc tgacgcggcg aagcacgcaa tacttctgcg ccaaaacgcg ccacgtcatg
    15001 caccgcggta aacgaagtca ccatctcaac gtaacagcag gaaagaccga agttatacgg
    15061 ccaaattgag tttttacgac cccagttaac catgtcattg agcttgccca taaacacgtt
    15121 tttgttaact tcttgctcca gagggtcggt tacgatctcc tgcttttgca gggggtaacg
    15181 gtcgttctca ccgttgggat ctatgcgggt gagcgtataa tccatcttaa tgcctcgcgg
    15241 ttagcgttga cgattagcga tactgttcgt ttccgggttc atacgctcgc ggcgtgaacg
    15301 cgcgggcgtc cagtccagcg cgccaatacg caccagataa accagacctg ccagtaacac
    15361 aaaaataaaa attgcagctt ccacaaagcc tacccagccg ctttcgcgga tagaggttga
    15421 ccatgcgaac agatacagcg cttcaacgtc gaagataacg aagaacatgg ccaccagata
    15481 aaacttggcg gacaggcgta agcgggcgga gccgaccgag tcgataccgg attcaaacgg
    15541 cacgtttttc gacctcgcgc gtgcgcgacc gcctaaaaac caaccgccta ccagcatcag
    15601 gcaacacagg ccaatggcaa cgataagaaa gatagcgaat gcccagtgat gagcgatgac
    15661 ttcagtggat gttgacatac tcattgctta ctcatcaaaa gtagcgccag attcactgct
    15721 cttcacggca gatggacgcc acatcgattc atggggagga ataaaaaaaa ccttacaatc
    15781 actgtagaaa ttcttttata cagctaattg
//
LOCUS       DAZKHF020000091        15752 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2201S31241
            SAMN43267800-rid25106413.denovo.092, whole genome shotgun sequence.
ACCESSION   DAZKHF020000091 DAZKHF020000000
VERSION     DAZKHF020000091.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267800
            Sequence Read Archive: SRR30307263
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 15752)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15752)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 15752)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350581.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350581.2
            Genome Coverage       :: 151x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:21:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,652
            CDSs (total)                      :: 5,557
            Genes (coding)                    :: 5,320
            CDSs (with protein)               :: 5,320
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 237
            CDSs (without protein)            :: 237
            Pseudo Genes (ambiguous residues) :: 0 of 237
            Pseudo Genes (frameshifted)       :: 102 of 237
            Pseudo Genes (incomplete)         :: 130 of 237
            Pseudo Genes (internal stop)      :: 52 of 237
            Pseudo Genes (multiple problems)  :: 41 of 237
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15752
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267800-rid25106413.denovo.092"
                     /serotype="O 26:H11"
                     /isolate="2201S31241"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-01"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            complement(100..1836)
                     /locus_tag="ACHOSC_RS11960"
                     /old_locus_tag="pgaptmp_002392"
     CDS             complement(100..1836)
                     /locus_tag="ACHOSC_RS11960"
                     /old_locus_tag="pgaptmp_002392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309713.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0051139 - metal cation:proton antiporter
                     activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA];
                     GO_process: GO:0006884 - cell volume homeostasis [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="potassium/proton antiporter"
                     /protein_id="HGU4548319.1"
                     /translation="MDATTIISLFILGSILVTSSILLSSFSSRLGIPILVIFLAIGML
                     AGVDGVGGIPFDNYPFAYMVSNLALAIILLDGGMRTQASSFRVALGPALSLATLGVLI
                     TSGLTGMMAAWLFNLDLIEGLLIGAIVGSTDAAAVFSLLGGKGLNERVGSTLEIESGS
                     NDPMAVFLTITLIAMIQQHESSVSWMFVVDILQQFGLGIVIGLGGGYLLLQMINRIAL
                     PAGLYPLLALSGGILIFALTTALEGSGILAVYLCGFLLGNRPIRNRYGILQNFDGLAW
                     LAQIAMFLVLGLLVNPSDLLPIAIPALILSAWMIFFARPLSVFAGLLPFRGFNLRERV
                     FISWVGLRGAVPIILAVFPMMAGLENARLFFNVAFFVVLVSLLLQGTSLSWAAKKAKV
                     VVPPVGRPVSRVGLDIHPENPWEQFVYQLSADKWCVGAALRDLHMPKETRIAALFRDN
                     QLLHPTGSTRLREGDVLCVIGRERDLPALGKLFSQSPPVALDQRFFGDFILEASAKYA
                     DVALIYGLEDGREYRDKQQTLGEIVQQLLGAAPVVGDQVEFAGMIWTVAEKEDNEVLK
                     IGVRVAEEEAES"
     gene            complement(1931..2845)
                     /gene="ldcA"
                     /locus_tag="ACHOSC_RS11965"
                     /old_locus_tag="pgaptmp_002393"
     CDS             complement(1931..2845)
                     /gene="ldcA"
                     /locus_tag="ACHOSC_RS11965"
                     /old_locus_tag="pgaptmp_002393"
                     /EC_number="3.4.17.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="muramoyltetrapeptide carboxypeptidase"
                     /protein_id="HGU4548320.1"
                     /translation="MSLFHLIAPSGYCIKQHAALRGIQRLTDEGHQVNNVEVIARRCE
                     RFAGTETERLEDLNSLARLTTPNTIVLAVRGGYGASRLLADIDWQALVARQQYDPLLI
                     CGHSDFTAIQCGLLAQGNVITFSGPMLVANFGADELNAFTEHHFWLALRNETFTIEWQ
                     GEGPTCQTEGTLWGGNLAMLISLIGTPWMPKIENGILVLEDINEHPFRVERMLLQLYH
                     AGILPRQKAIILGSFSGSTPNDYDAGYNLESVYAFLRSRLSIPLITGLDFGHEQRTVT
                     LPLGAHAILNNTREGTQLTISGHPVLKM"
     gene            2945..3556
                     /gene="emtA"
                     /locus_tag="ACHOSC_RS11970"
                     /old_locus_tag="pgaptmp_002394"
     CDS             2945..3556
                     /gene="emtA"
                     /locus_tag="ACHOSC_RS11970"
                     /old_locus_tag="pgaptmp_002394"
                     /EC_number="4.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309714.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane-bound lytic murein transglycosylase
                     EmtA"
                     /protein_id="HGU4548321.1"
                     /translation="MKLRWFAFLIVLLAGCSSKHDYTNPPWNAKVPVQRAMQWMPISQ
                     KAGAAWGVDPQLITAIIAIESGGNPNAVSKSNAIGLMQLKASTSGRDVYRRMGWSGEP
                     TTSELKNPERNISMGAAYLNILETGPLAGIEDPKVLQYALVVSYANGAGALLRTFSSD
                     RKKAISKINDLDADEFLDHVARNHPAPQAPRYIYKLEQALDAM"
     gene            complement(3558..4292)
                     /locus_tag="ACHOSC_RS11975"
                     /old_locus_tag="pgaptmp_002395"
     CDS             complement(3558..4292)
                     /locus_tag="ACHOSC_RS11975"
                     /old_locus_tag="pgaptmp_002395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar brake protein"
                     /protein_id="HGU4548322.1"
                     /translation="MSHYHEQFLKQNPLAVLGVLRDLHKAAIPLRLSWNGGQLISKIL
                     AITPDKLVLDFGSQAEDNIAVLKAQHITITAETQGAKVEFTVEQLQQSEYLQLPAFIT
                     VPPPTLWFVQRRRYFRISAPLHPPYFCQTKLADNSTLRFRLYDLSLGGMGALLETAKP
                     AELQEGMRFAQIEVNMGQWGVFHFDAQLISISERKVIDGKNETITTPRLSFRFLNVSP
                     TVERQLQRIIFSLEREAREKADKVRD"
     gene            4493..4747
                     /locus_tag="ACHOSC_RS11980"
                     /old_locus_tag="pgaptmp_002396"
     CDS             4493..4747
                     /locus_tag="ACHOSC_RS11980"
                     /old_locus_tag="pgaptmp_002396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HGU4548323.1"
                     /translation="MGIIAWIIFGLIAGIIAKLIMPGRDGGGFFLTCILGIVGAVVGG
                     WLATMFGIGGSISGFNLHSFLVAVVGAILVLGVFRLLRRE"
     gene            4925..5365
                     /gene="ycgY"
                     /locus_tag="ACHOSC_RS11985"
                     /old_locus_tag="pgaptmp_002397"
     CDS             4925..5365
                     /gene="ycgY"
                     /locus_tag="ACHOSC_RS11985"
                     /old_locus_tag="pgaptmp_002397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YcgY"
                     /protein_id="HGU4548324.1"
                     /translation="MIQKINILDSSQSGVKNGADSEAVLAALIEHLELINPSGRLSQN
                     TRSAMLQLREEEWSEFFFWLLNSLECLDYVIINLAPESKKTLMSEHRNNIQVAIDALY
                     RQRRCKSPGDESETLTRRNDAIFGNHVWQTFAQYFPPGLEKPSV"
     gene            complement(5444..7141)
                     /gene="treA"
                     /locus_tag="ACHOSC_RS11990"
                     /old_locus_tag="pgaptmp_002398"
     CDS             complement(5444..7141)
                     /gene="treA"
                     /locus_tag="ACHOSC_RS11990"
                     /old_locus_tag="pgaptmp_002398"
                     /EC_number="3.2.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha,alpha-trehalase"
                     /protein_id="HGU4548325.1"
                     /translation="MKSPAPSRPQKMALIPACIFLCFAALSVQAEETPVTPQPPDILL
                     GPLFNDVQNAKLFPDQKTFADAVPNSDPLMILADYRMQQNQSGFDLRHFVNVNFTLPK
                     EGEKYVPPEGQSLREHIDGLWPVLTRSTENTEKWDSLLPLPEPYVVPGGRFREVYYWD
                     SYFTMLGLAESGHWDKVADMVANFAHEIDTYGHIPNGNRSYYLSRSQPPFFALMVELL
                     AQHEGDAALKQYLPQMQKEYAYWMDGVENLQAGQQEKRVVKLQDGTLLNRYWDDRDTP
                     RPESWVEDIATAKSNPNRPATEIYRDLRSAAASGWDFSSRWMDNPHQLNTLRTTSIVP
                     VDLNSLMFKMEKILARASKAAGDNAMANQYETLANARQKGIEKYMWNDQQGWYADYDL
                     KSHKVRNQLTAAALFPLYVNAAAKDRANKMATATKTHLLQPGGLNTTSVKSGQQWDAP
                     NGWAPLQWVATEGLQNYGQKEVAMDISWHFLTNVQHTYDREKKLVEKYDVSTTGTGGG
                     GGEYPLQDGFGWTNGVTLKMLDLICPKEQPCDNVPATRPTVKSATTQPSTKEAQPTP"
     gene            complement(7461..8879)
                     /gene="dhaM"
                     /locus_tag="ACHOSC_RS11995"
                     /old_locus_tag="pgaptmp_002399"
     CDS             complement(7461..8879)
                     /gene="dhaM"
                     /locus_tag="ACHOSC_RS11995"
                     /old_locus_tag="pgaptmp_002399"
                     /EC_number="2.7.1.121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415716.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0047324 - phosphoenolpyruvate-glycerone
                     phosphotransferase activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA];
                     GO_process: GO:0019563 - glycerol catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxyacetone kinase phosphoryl donor subunit
                     DhaM"
                     /protein_id="HGU4548326.1"
                     /translation="MVNLVIVSHSSRLGEGVGELARQMLMSDSCKIAIAAGIDDPQNP
                     IGTDAVKVMEAIESVADADHVLVMMDMGSALLSAETALELLAPKIAAKVRLCAAPLVE
                     GTLAATVSAASGADIDKVIFDAMHALEAKREQLGLPSSDTEISDTCPAYDEEARSLAV
                     VIKNRNGLHVRPASRLVYTLSTFNADMLLEKNGKCVTPESINQIALLQVRYNDTLRLI
                     AKGPEAEEALIAFRQLAEDNFGETEEVAPPTLRPVPPVSGKAFYYQPVLCTVQAKSTL
                     TVEEEQDRLRQAIDFTLLDLMTLTAKAEASGLDDIAAIFSGHHTLLDDPELLAAASEL
                     LQHEHCTAEYAWQQVLKELSQQYQQLDDEYLQARYIDVDDLLHRTLVHLTQTKEELPQ
                     FNSPTILLAENIYPSTVLQLDPAVVKGICLSAGSPVSHSALIARELGIGWICQQGEKL
                     YAIQPEETLTLDVKTQRFNRQG"
     gene            complement(8890..9522)
                     /gene="dhaL"
                     /locus_tag="ACHOSC_RS12000"
                     /old_locus_tag="pgaptmp_002400"
     CDS             complement(8890..9522)
                     /gene="dhaL"
                     /locus_tag="ACHOSC_RS12000"
                     /old_locus_tag="pgaptmp_002400"
                     /EC_number="2.7.1.121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047324 - phosphoenolpyruvate-glycerone
                     phosphotransferase activity [Evidence IEA];
                     GO_process: GO:0006071 - glycerol metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxyacetone kinase subunit DhaL"
                     /protein_id="HGU4548327.1"
                     /translation="MSLSRTQIVNWLTRCGDIFSTESEYLTGLDREIGDADHGLNMNR
                     GFSKVVEKLPAIADKDIGFILKNTGMTLLSSVGGASGPLFGTFFIRAAQATQARQSLT
                     LEELYQMFRDGADGVISRGKAEPGDKTMCDVWVPVVESLRQSSEQNLSVPVALEAASR
                     IAESAAQSTITMQARKGRASYLGERSIGHQDPGATSVMFMMQMLALAAKE"
     gene            complement(9533..10603)
                     /gene="dhaK"
                     /locus_tag="ACHOSC_RS12005"
                     /old_locus_tag="pgaptmp_002401"
     CDS             complement(9533..10603)
                     /gene="dhaK"
                     /locus_tag="ACHOSC_RS12005"
                     /old_locus_tag="pgaptmp_002401"
                     /EC_number="2.7.1.121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415718.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004371 - glycerone kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006071 - glycerol metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxyacetone kinase subunit DhaK"
                     /protein_id="HGU4548328.1"
                     /translation="MKKLINDVQDVLDEQLAGLAKAHPSLTLHQDPVYVTRADAPVAG
                     KVALLSGGGSGHEPMHCGYIGQGMLSGACPGEIFTSPTPDKIFECSMQIDGGEGVLLI
                     IKNYTGDILNFETATELLHDSGVKVTTVVIDDDVAVKDSLYTAGRRGVANTVLIEKLV
                     GAAAERGDSLDACAELGRKLNNQGHSIGIALGACTVPAAGKPSFTLADNEMEFGVGIH
                     GEPGIDRRSFSSLDQTVDEMFDTLLENGSYHRTLRFWDYQQGSWQEEQQTKQPLQSGD
                     RVIALVNNLGATPLSELYGVYNRLTTRCQQAGLTIERNLIGAYCTSLDMTGFSITLLK
                     VDDETLALWDAPVHTPALNWGK"
     gene            10831..12750
                     /gene="dhaR"
                     /locus_tag="ACHOSC_RS12010"
                     /old_locus_tag="pgaptmp_002402"
     CDS             10831..12750
                     /gene="dhaR"
                     /locus_tag="ACHOSC_RS12010"
                     /old_locus_tag="pgaptmp_002402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415719.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxyacetone kinase operon transcriptional
                     regulator DhaR"
                     /protein_id="HGU4548329.1"
                     /translation="MSGAFNNDGRGISPLIATSWERCNKLMKRETWNVPHQAQGVTFA
                     SIYRRKKAMLTLGQAALEDAWEYMAPRECALFILDETACILSRNGDPQTLQQLSALGF
                     NDGTYCAEGIIGTCALSLAAISGQAVKTMADQHFKQALWNWAFCATPLFDSKGRLTGT
                     IALACPVEQTTAADLPLTLAIAREVGNLLLTDSLLAETNRHLNQLNALLESMDDGVIS
                     WDEQGNLQFINAQAARVLRLDATASQGRAITELLTLPAVLQQAIKQAHPLKHVEATFE
                     SQHQFIDAVITLKPIIETQGTSFILLLHPVEQMRQLMTSQLGKVSHTFAHMPQDDPQT
                     RRLIHFGRQAARSSFPVLLCGEEGVGKALLSQAIHNESERAAGPYIAVNCELYGDAAL
                     AEEFIGGDRTDNENGRLSRLELAHGGTLFLEKIEYLAVELQSALLQVIKQGVITRLDA
                     RRLIPVDVKVIATTTADLAMLVEQNRFSRQLYYALHAFEITIPPLRMRRGSIPALVNN
                     KLRSLEKRFSTRLKIDDDALARLVSCAWPGNDFELYSVIENLALSSDNGRIRVSDLPE
                     HLFTEQATDDVSATRLSTSLSFAEVEKEAIINAAQVTGGRIQEMSALLGIGRTTLWRK
                     MKQHGIDAGQFKRRV"
     gene            complement(12850..15735)
                     /locus_tag="ACHOSC_RS12015"
                     /old_locus_tag="pgaptmp_002403"
     CDS             complement(12850..15735)
                     /locus_tag="ACHOSC_RS12015"
                     /old_locus_tag="pgaptmp_002403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter outer membrane beta-barrel
                     domain-containing protein"
                     /protein_id="HGU4548330.1"
                     /translation="MGIKQHNGNTKADRLAELKIRSPSIQLIKFGAIGLNAIIFSPLL
                     IAADTGSQYGTNITINEGDRITGDTADPSGNLYGVMTPAGNTPGNINLGNDVTVNVND
                     ASGYAKGIIIQGKNSSLTANRLTVDVVGQTSAIGINLIGDYTHADLGTGSTIKSNDDG
                     IIIGHSSTLTATQFTIENSNGIGLTINDYGTSVDLGSGSKITTDGSTGVYIGGLNGNN
                     ANGAARFTATDLTIDVQGYSAMGINVQKNSVVDLGTNSSIKTNGDNAHGLWSFGQVSA
                     NALTVDVTGAAANGVEVRGGTTTIGADSHISSAQGGGLVTSGSDATINFSGTAAQRNS
                     IFSGGSYGASAQTATAVVNMQNTDITVDRNGSLALGLWALSGGRITGDSLAITGAAGA
                     RGIYAMTNSQIDLTSDLVIDMSTPDQMAIATQHDDGYAASRINASGRMLINGSVLSKG
                     GLINLDMHPGSVWTGSSLSDNVNGGKLDVAMNNSVWNVTSNSNLDTLALSHSTVDFAS
                     HGSTAGTFATLNVENLSGNSTFIMRADVVGEGNGVNNKGDLLNISGSSAGNHVLAIRN
                     QGSEATTGNEVLTVVKTTDGAASFSASSQVELGGYLYDVRKNGTNWELYASGTVPEPT
                     PNPEPTPAPAQPPIVNPDPTPEPDPTPNPTPTPKPTTTADAGGNYLNVGYLLNYVENR
                     TLMQRMGDLRNQSKDGNIWLRSYGGSLDSFASGKLSGFDMGYSGIQFGGDKRLSDVMP
                     LYVGLYIGSTHASPDYSGGDGTARSDYMGMYASYMAHNGFYSDLVVKASRQKNSFHVL
                     DSQNNGVNANGTANGLSISLEAGQRFNLTPTGYGFYIEPQTQLTYSHQNEMAMKASNG
                     LNIHLNHYESLLGRASMILGYDITAGNSQLNMYVKTGAIREFSGDTDYLLNNSREKYS
                     FKGNGWNNGVGVSAQYNKQHTFYLEADYTQSNLFDQKQVNGGYRFSF"
BASE COUNT         3856 a         3946 c         3988 g         3962 t
ORIGIN      
        1 ccaacatcaa atgaaggtta aattatggac gacaaacaat tgcaggctca ggctgcgttc
       61 agcaaagcat cgcaaccggc gatagatgct tcattaaatt taagattcag cttcctcttc
      121 agccacccgt acgccaatct tcaacacttc attatcttct ttctcggcca ccgtccagat
      181 catcccggca aactctacct ggtcaccgac aaccggtgcc gcgcctaaca actgctggac
      241 aatttcaccc agcgtttgct gcttatcacg atattctcgc ccgtcttcca ggccatatat
      301 cagcgcaaca tcagcatatt tggcgctggc ttcgagaatg aagtcaccaa agaagcgttg
      361 atccagcgcg accggcggcg actggctgaa cagtttaccg agcgccggaa gatcgcgttc
      421 ccgaccaatt acacacaaca catcgccttc gcgcagtcgg gtgctgccgg taggatgaag
      481 caactggtta tcacgaaaca gtgccgcaat acgcgtctct tttggcatat gcaaatcgcg
      541 cagtgccgcg cccacgcacc atttatcggc actcaattga taaacaaact gctcccacgg
      601 attttccgga tgaatatcca ggccaacgcg tgacaccgga cgtcccactg gcggaaccac
      661 cactttggct tttttagccg cccacgagag tgatgttccc tgcaagagca gtgaaaccag
      721 aaccacaaag aaggcgacat taaagaacag acgtgcattc tccagccccg ccatcatcgg
      781 gaacacagcc aggatgattg gcaccgcgcc gcgtaatcct acccagctga taaacacgcg
      841 ctcacgcaga ttgaaaccgc ggaaggggag caatccagca aataccgaaa ggggacgggc
      901 gaagaatatc atccatgccg acaaaatgag cgccggaatg gcaataggca gcagatcgct
      961 tgggttaacc aacagcccca gcaccaggaa catggcgatt tgcgccagcc aggcgaggcc
     1021 gtcgaaattt tgcaggatgc cgtagcggtt gcgaatcggc cgattaccca gcagaaaacc
     1081 gcacagataa acagccagaa taccgctgcc ttccagcgcc gtggttaaag caaaaattaa
     1141 aataccgccg cttaatgcca gcaatggata taatccggcg ggcagggcga tgcgattaat
     1201 catttgcagc agtaaataac caccaccaag tccgatgaca atcccgaggc caaattgctg
     1261 cagaatatcg accacgaaca tccaactgac actgctttca tgttgctgga tcatcgcaat
     1321 cagggtaatc gtcagaaaga ccgccattgg atcattactg ccggattcaa tttccagcgt
     1381 cgagccaaca cgttcgttaa gccccttacc acccagcaga gaaaagaccg ctgcagcatc
     1441 ggtagagccg acgatagcgc cgattaataa gccttcaatc aaatcaagat taaacagcca
     1501 cgccgccatc atgccggtta aaccagaggt gataagcacg cccagcgtcg ccagcgacag
     1561 tgccggtcct aacgccacac gaaaggaact ggcctgagtg cgcatcccgc cgtcgagcaa
     1621 aataatcgcc agtgccaggt tacttaccat gtaggcgaag gggtaattat caaacgggat
     1681 accgccgacg ccatcgactc ctgccagcat gccgatcgcc agaaaaataa ccagaatagg
     1741 aatgccaaga cgggaagaaa atgaactaag taatatactg ctggtgacaa ggatggatcc
     1801 taagatgaaa aggctaatta ttgttgtggc atccaacggt cggttactcc tgattacgct
     1861 gtctcttata taaaccctac catattagcg gcagagacag cgttttactt agccctgaag
     1921 cgtgattttt ttacatttta agaacaggat gaccgctaat cgtcaactga gtgccttccc
     1981 gggtgttatt cagaatggca tgtgcgccca acggcagcgt aaccgtgcgt tgttcatgac
     2041 caaaatcgag gccggtaatg agtggaatcg acaggcggga acgcaaaaac gcgtacactg
     2101 actccaggtt gtaacctgcg tcataatcat tgggcgtgct gccgctaaag ctaccgagaa
     2161 taatcgcctt ctgacgtggc aaaattcccg catgatagag ctgcaacagc atacgttcga
     2221 cgcggaaagg gtgctcgtta atatcttcca gcaccagaat accgttctca atttttggca
     2281 tccacggtgt accaatcagt gaaatcagca tcgcaagatt gcctccccac aacgtgcctt
     2341 cggtctgaca tgtcgggcct tccccttgcc attcaatggt gaaggtttca ttgcgtaacg
     2401 ccagccagaa atggtgctcg gtaaaggcgt tcagttcatc cgcgccaaag tttgctacca
     2461 gcatggggcc gctaaaggtg atgacattgc cttgcgccag aagaccgcac tgaatggcgg
     2521 taaaatcgct atgcccgcat atgagcaacg ggtcatattg ttggcgagca accagtgcct
     2581 gccagtcaat atctgccagt aaacgactgg caccgtaacc gccgcgtaca gccagcacga
     2641 tggtgttggg ggtggtcagt ctggcaaggg aattgagatc ttccaggcgc tccgtttccg
     2701 tacccgcaaa acgctcacaa cgacgggcaa taacctcgac gttattgacc tgatgcccct
     2761 cgtcggtaag gcgctggata ccacgcagcg cggcgtgctg tttaatgcag taacccgatg
     2821 gggcaattaa gtgaaacaga gacatggcaa ttccttgctg acaacagaaa cgaaatgtat
     2881 atcatgccgc ttaggtgtgc cgttgtcacc tcaacggcga ttccaggcta taaggataga
     2941 agaagtgaaa ttgagatggt ttgccttttt gattgtgtta ttagcgggtt gttcatcaaa
     3001 gcatgactat acgaacccgc cgtggaacgc gaaagttccg gtgcaacgtg cgatgcagtg
     3061 gatgccaata agccagaaag ccggtgcagc ctggggcgtc gatccacaat tgatcacggc
     3121 gattatcgct atcgaatcgg gtggtaatcc gaatgcggtg agtaaatcga atgccattgg
     3181 tttgatgcag ttaaaagctt caacctccgg acgtgatgtt tatcgccgta tgggctggag
     3241 tggtgagccg acgaccagcg agctgaaaaa tccagagcgt aatatttcaa tgggggcggc
     3301 ttacctgaat attctggaaa ccggcccgct ggcaggcatt gaagatccga aggtactgca
     3361 atatgcgctg gtggtgtcat acgctaacgg ggcaggtgcg ctgctacgga ctttctcgtc
     3421 agatcggaaa aaggcgatca gcaaaatcaa cgatttggat gctgacgagt tcctcgatca
     3481 cgtagcgcga aatcatcctg cgccgcaggc tccgcgctat atctacaaac ttgagcaggc
     3541 actggacgcg atgtaaatca gtcgcgcact ttgtccgctt tttcccgggc ttctcgctcg
     3601 agagagaaaa taatccgctg taattgccgc tccaccgtcg ggctgacgtt aagaaaacgg
     3661 aagctcagac ggggagtggt gatggtttca ttcttgccat caatcacttt gcgctcgctg
     3721 atggagatta actgggcgtc aaagtgaaaa acaccccatt gccccatgtt gacttcaatc
     3781 tgagcgaagc gcatgccttc ttgtaattcg gcaggctttg ctgtttccag taatgcgccc
     3841 atgccgccta acgacaaatc atacaggcgg aaacgtaacg tactgttatc cgccagtttg
     3901 gtctggcaaa aataaggcgg atggagtggg gcggagatgc ggaaatatcg gcgtcgttgt
     3961 acaaaccata aggtgggagg cggtacggtg ataaatgccg gaagctgcaa gtattcactc
     4021 tgctgtagtt gttcaacagt aaactcgact ttcgcaccct gagtttcggc ggtaatggta
     4081 atgtgctgtg cctttagcac ggcgatgttg tcttcggctt gactgccgaa atccagcacc
     4141 agtttatccg gggttattgc cagtattttg ctgatcagct gcccgccatt ccaactgaga
     4201 cgcaaaggaa ttgcggcttt gtgcaaatcg cgtaacacgc ccaggacggc taacggattt
     4261 tgtttcagga actgctcatg gtaatgactc acgcggaaaa actcctgact gacaaactgt
     4321 ctttggttta tcggtcacag ttaacaaaac ttaatacaaa tgcgtgaata ttttttacat
     4381 gttgttctta aatcagccga catacgccca gcatcgatcc cgcccttgcc tatacttaga
     4441 gcgttgacgt aagcatttct tgcgtccgat tcatcgaaac gagggcctga acatgggaat
     4501 tattgcctgg attatttttg gcctgatagc cggcattatc gccaagctaa tcatgccggg
     4561 gcgtgatggt ggtggatttt tcctgacctg tattctcggg atagtcggtg cggtggtcgg
     4621 cggctggctg gcgaccatgt ttggcattgg cggctccatc agtggtttta atctgcacag
     4681 cttcctggtg gcggtggtgg gagctattct cgttctgggc gtattccgcc tcctgcgaag
     4741 agaataagat tttcataagg cggatagcga tacagatgcc gctatccgct ttcacatcag
     4801 aacgtatact cgacacctgc tttacgggtg gaacaaatca atatagcact tagatgcccg
     4861 ataaagtcgg ttcccggtaa caacaacaga atcctttttt aattattgtt tcgttgtttt
     4921 tggtgtgatt cagaaaataa atattttaga cagtagccag tcaggcgtta aaaatggggc
     4981 ggatagtgag gcggttttgg ctgcgcttat tgaacattta gaattgatca atccttctgg
     5041 cagattaagc caaaataccc gttcggcaat gcttcagtta cgcgaagagg aatggtctga
     5101 attctttttt tggttgctaa attctctgga gtgtctggat tatgtgatta ttaatttagc
     5161 tccagaaagc aaaaaaacgt tgatgagtga acaccgtaac aatattcagg tagcaatcga
     5221 tgctttatat aggcaacgtc ggtgtaaatc accgggtgat gaaagtgaaa cacttacccg
     5281 tagaaatgac gctatcttcg gcaaccacgt atggcaaaca tttgcgcaat acttcccgcc
     5341 aggacttgaa aaaccgtctg tgtagaaaac accatagcta tggtgttatc tgccgacaac
     5401 gccatccgga gaagccgaat gacgttgtcg gagtaagcgc tggttaaggt gtgggttgtg
     5461 cctctttggt tgagggttgc gtcgttgctg acttaacggt cggacgcgtc gccggaacat
     5521 tgtcacacgg ttgctctttc gggcagatca aatccagcat tttcagcgtc acgccattgg
     5581 tccagccaaa gccatcctgt aatggatatt cgccaccgcc gccccccgtt ccggtggtgc
     5641 tgacatcata tttttccacc agctttttct cccggtcata ggtgtgctga acattggtca
     5701 ggaagtgcca gctaatgtcc atcgccacct ctttttgccc gtagttttgt aatccttctg
     5761 tcgcgaccca ctgtaacggt gcccagccat ttggcgcatc ccattgttgc ccacttttca
     5821 ccgacgtggt gttcaggccg ccgggttgca gcagatgtgt tttcgtcgcc gtcgccattt
     5881 tgttggcgcg atctttcgct gccgcattga cgtacagcgg gaacagggcg gccgcggtta
     5941 actgattgcg cactttatga cttttcaggt cgtaatcggc ataccagcct tgttgatcgt
     6001 tccacatgta tttttcgatc cctttttgac gggcatttgc cagcgtttcg tactggtttg
     6061 ccatcgcgtt atctccggca gctttgctgg cgcgggcgag gattttttcc attttaaaca
     6121 tcaggctgtt cagatcgacc ggtacgatgc tggtggtgcg taaggtattt agttgatgcg
     6181 ggttgtccat ccagcgcgag ctgaaatccc agccagacgc agcggcagag cgcaggtcgc
     6241 ggtaaatttc agtggcaggt cgattcggat tgcttttggc ggtggcaata tcttccaccc
     6301 atgactctgg tcgtggcgta tcgcgatcgt cccagtagcg gttgagaagg gtaccatcct
     6361 gaagttttac aacgcgtttt tcctgttgtc cggcttgcag gttttcaaca ccgtccatcc
     6421 agtaagcata ttctttttgc atttgcggca ggtattgctt caacgcggca tcgccttcat
     6481 gctgcgccag taactctacc atcagggcaa agaagggcgg ttgcgagcgg cttaaatagt
     6541 aactgcggtt gccgttggga atatgaccgt aagtgtctat ttcatgagca aaattggcca
     6601 ccatatccgc gactttatcc cagtgaccgc tttcggcaag tcctaacatg gtgaagtaac
     6661 tgtcccagta atatacctcg cgaaaacgtc cgcccggcac gacataaggt tccggcagcg
     6721 gtaacagaga atcccatttt tcggtgtttt cggtagaacg cgttaatacc ggccaaagtc
     6781 cgtcaatatg ttcgcgcagt gactgcccct ctggcggaac atatttctcg ccctctttcg
     6841 gcagggtgaa attgacgtta acgaaatggc gcagatcaaa tccgctctgg ttttgctgca
     6901 tccgataatc agcaaggatc atcagcggat cgctgttcgg cacggcatcg gcaaaggttt
     6961 tttggtccgg aaaaagtttg gcgttttgca catcattaaa cagcggccct aataaaatat
     7021 caggcggctg tggtgttacc ggtgtttctt ctgcctgcac cgatagcgca gcgaaacaca
     7081 aaaagataca ggctggaatt aacgccattt tttgcgggcg agaaggtgcg ggggatttca
     7141 tcaatcattc tcctttggcg aaaccgaata aaacggtatt caagtatcag aaaaccttag
     7201 ttcaggatcg cactagctgc atgatcgaaa tctcattctg cgaactgtca ggcattttag
     7261 aaaagggaat taaagggata aataacggtg aaatagagtg atcaaataac gtctgtttat
     7321 tagccagtca gcgacgttgg gttcgcgatt agcgaagaaa aatcatcata aattggtaaa
     7381 aaatataaca ggtagttaaa aaacatcagt gctgggtaaa ttgccggatg acatcagaac
     7441 gatgccatcc gaacggtggc ttaaccctga cggttgaaac gttgcgtttt aacgtccagc
     7501 gttagcgttt cttctggttg tatcgcatac agtttctcac cctgctggca aatccagcca
     7561 atccccagtt cacgggcgat tagggcgctg tgggataccg gacttccggc gctaaggcag
     7621 atacctttta caaccgccgg atccagttgc agtactgtgg aaggataaat gttctccgcc
     7681 agtagaatag ttggcgagtt aaactgcggg agttcttctt tcgtttgggt caggtggacc
     7741 agggtgcgat gcagaagatc gtccacatca atatagcgag cttgtagata ttcatcatcc
     7801 agttgctggt attgctggct aagttcttta agaacttgct gccaggcata ttctgccgtg
     7861 caatgttcat gctgaaggag ttcgcttgcc gccgccagca gttccggatc atctaacagt
     7921 gtatggtgac cagaaaagat tgcggcaata tcgtcaagcc cgctggcttc tgctttcgct
     7981 gttaacgtca tcagatctaa taacgtgaag tcaatagcct ggcgtaatcg atcttgttct
     8041 tcttccacgg tcagggttga ttttgcctgt accgtacata aaactggttg ataataaaag
     8101 gctttacccg aaacaggcgg aacgggacgc agagtaggtg gagcgacttc ctccgtttca
     8161 ccaaagttat cttcagccag ctgacggaaa gcgatcagtg cctcttcagc ttctggccct
     8221 ttcgcaatca ggcgcagcgt atcgttatag cgaacttgta gtaacgcaat ctggttaata
     8281 ctctctggtg tgacgcattt gccgtttttt tccagcaaca tatcggcatt aaatgtcgat
     8341 aaggtataaa ccagccggga ggccggacgt acatgcaggc cgttacggtt ttttatgacc
     8401 accgccagag aacgggcttc ttcatcgtac gcaggacatg tgtcagagat ttcagtgtcg
     8461 gaggacggta aacccagttg ttcacgtttg gcttccagcg catgcatagc gtcaaagata
     8521 actttgtcga tatccgctcc cgaggccgcg ctgaccgttg ctgccagtgt accttcgacc
     8581 aacggcgcag cacacaaacg tacttttgcg gcgatcttgg gagccagcaa ttccagcgca
     8641 gtttcagcac tcaataatgc gctacccata tccatcatga ccagcacatg gtcggcatca
     8701 gcaacagatt cgatggcctc catcactttg acggcatcgg taccaatagg attttgtgga
     8761 tcgtcaattc ccgcggcaat ggcgatttta caactatcac tcattaacat ctgacgggct
     8821 aattcaccga caccttctcc cagtcggctg ctatgtgaaa ctatgaccag gtttaccatc
     8881 accaattcct tactcttttg cggctaacgc caacatttgc atcataaaca tcaccgaggt
     8941 cgcgccggga tcctggtgac caatactgcg ttcaccgaga taactggcgc ggcctttgcg
     9001 ggcttgcatc gtaatcgtac tttgtgcagc ggattcggcg attctactgg cagcttcgag
     9061 cgccaccgga acagagagat tttgctcgct ggactgacgt aacgattcca ccaccggcac
     9121 ccacacatca cacatggttt tatcgccagg ttcggctttc ccgcgactga ttacgccgtc
     9181 cgcgccatcg cggaacatct gataaagctc ttccagtgtc aggctttgcc gtgcctgggt
     9241 cgcctgtgcg gcgcggataa agaaggtacc gaacagcgga ccactggcac caccgacgct
     9301 ggaaagcagc gtcataccgg tattcttgag aatgaaaccg atatctttat ctgcgatagc
     9361 agggagtttt tccaccactt tgctaaagcc tcggttcata tttagcccgt ggtcagcgtc
     9421 gccaatttcg cgatccagtc cggtaagata ctcgctctcg gtgctgaaaa tatcgccaca
     9481 acgagtgagc cagttaacaa tttgagttct gctcagtgac attgctttct ccttatttac
     9541 cccagttaag ggccggggtg tggaccgggg cgtcccagag tgccagcgtt tcgtcatcaa
     9601 ctttcagtaa ggtgattgag aaaccggtca tatccagtga ggtgcagtac gcgccaatta
     9661 aattacgttc gatagtcaat cccgcttgct ggcaacgtgt ggtcaggcgg ttatagacgc
     9721 cgtacagctc agaaagcgga gttgcgccaa gattgttaac cagcgcaatc acccgatcgc
     9781 cagactggag cggttgtttg gtttgttgtt cttcctgcca actgccttgt tgataatccc
     9841 agaaacgcaa agtgcgatgg tatgagccat tttccagcag ggtgtcgaac atttcatcga
     9901 cggtttgatc aagggaagag aaggagcggc ggtcaatacc cggctcacca tgaatgccga
     9961 cgccaaactc catctcatta tccgccaggg taaaagaagg tttgcccgcg gcaggaacgg
    10021 tacaggcacc gagagcgata cctattgagt ggccttgatt attcaactta cgccccagtt
    10081 ccgcacaggc gtccagtgag tcgccacgct ccgccgctgc gcctacgagt ttttcaatta
    10141 atacggtgtt ggcaacgccg cgtcgcccgg cggtataaag actgtctttt accgcaacgt
    10201 cgtcatcaat gaccacagtg gtcactttta cgccgctatc gtgcagtaac tcggtcgctg
    10261 tttcaaagtt aagaatatcg ccggtgtaat ttttgataat caacagtaca ccttcgccgc
    10321 catcaatttg catggagcat tcaaagattt tatcgggcgt cggtgaggtg aaaatttcgc
    10381 ccggacaggc ccccgaaagc atcccctgac cgatataacc acagtgcatc ggctcgtgtc
    10441 cgctgccgcc acccgacagc agggcgactt ttcctgcaac aggggcatca gctcgggtga
    10501 catacaccgg atcctgatgc agtgtcagcg atggatgcgc tttcgccagt cctgccagtt
    10561 gttcgtccag tacgtcttgc acatcattga tcaatttttt cattattttg ctccagcaat
    10621 tacggtaggg catggatgat gttcaacgac acggcgacca ctgactgccg atgaatccat
    10681 tgtgcatcag gcaagggaaa agaaaattag cccaaattat gtttcatagt gaaacatatg
    10741 ctttaatgaa tgttccatat tgaaactttt acgtgtatta atacttaaaa ttgcgagccg
    10801 gaacaccttt tgtcataagg gatgcgggat atgagtggcg cttttaacaa cgatggtcgg
    10861 ggcatatctc ccttaattgc aacctcctgg gagcgatgca ataagctgat gaaacgggag
    10921 acatggaacg taccacatca ggcccagggc gtgacatttg cttctattta tcggcgtaag
    10981 aaagcgatgc tgacgctcgg gcaggctgcg ctggaagatg cctgggaata tatggcaccg
    11041 cgagagtgtg cgctgtttat cctcgatgaa accgcctgca ttctcagccg taatggcgat
    11101 ccgcaaacct tgcagcagct aagtgcactg ggattcaatg acggcacgta ttgcgccgag
    11161 ggaattattg gtacttgtgc gctatcgtta gcggctatct ctggtcaggc cgtgaaaacg
    11221 atggccgatc aacatttcaa acaggcactc tggaactggg ccttttgtgc aacgccgttg
    11281 tttgacagca agggccgatt gacgggaaca atagcgctgg cgtgtccggt tgagcaaact
    11341 accgcagctg atttgccgtt gacgttggca atcgcccgcg aggtcggaaa tttactgctg
    11401 acggacagtt tgctcgctga aactaaccgt catttaaatc aacttaatgc cctgttagaa
    11461 agtatggatg atggcgtgat tagctgggac gagcagggta atttgcaatt tattaatgcc
    11521 caggcggcgc gggtcttgcg ccttgacgcg acggcaagtc agggaagggc aatcactgaa
    11581 ctcttaacgt tacccgccgt attgcaacaa gcaataaaac aggcacatcc gctcaaacac
    11641 gtagaagcaa cctttgaaag tcagcatcag tttattgatg cggtgataac ccttaaaccg
    11701 ataatagaaa cgcagggaac cagctttatt ttgttgctcc atcctgtgga acagatgcgg
    11761 cagttgatga ccagtcaatt aggaaaagtc agccatacct tcgctcatat gccacaggac
    11821 gatccgcaaa cccgccgctt gattcatttt ggtcgccagg cggcgcgcag tagctttcct
    11881 gtcctgcttt gtggagaaga gggcgtggga aaggcactgc taagtcaggc aattcataat
    11941 gaaagcgagc gtgctgcagg tccttatatc gccgtcaatt gtgagttata tggtgatgcg
    12001 gcgctggcgg aagaatttat tggtggcgat cgcacggaca atgaaaatgg ccgtctgagt
    12061 cggctggaac tggcacacgg cggcacgctg tttcttgaaa agattgaata tctggcggtg
    12121 gagttacagt ctgctttgct tcaggttatc aagcagggtg ttatcacgcg actggatgcg
    12181 cggcgtttaa taccagttga tgtcaaagtg attgccacaa cgaccgcgga cctcgcaatg
    12241 ctggtggaac aaaatcgttt tagtcgccag ctgtattacg cgttgcatgc atttgaaatt
    12301 accatcccgc ctctgcgtat gcggcgtggc agcattccgg cgctggtgaa taacaaatta
    12361 cgcagtcttg aaaaacgctt ctctacgcgg ctgaaaattg atgacgatgc cctcgctcgc
    12421 ctggtttcct gtgcatggcc tggcaacgat tttgaacttt acagcgtcat cgagaatctt
    12481 gctctgagta gtgataatgg gcgcattcgc gtcagtgatt tgccggaaca tctgtttacc
    12541 gagcaggcaa cagatgatgt cagcgccaca cgcctttcca ccagtctgtc atttgcggaa
    12601 gttgaaaaag aggcaattat taacgcagcc caggtcacag gcggtcgcat tcaggaaatg
    12661 tcggctttac ttgggatcgg ccgcactacg ctgtggcgga aaatgaagca acatggcatt
    12721 gatgcagggc agtttaagcg ccgggtatga aagacagaga cgatttctga tacatcagag
    12781 tgatctatat ttcattccgg cgcacgctaa catttttcag catcgtttaa gggctggtct
    12841 atcccgcact taaaagctga agcgatatcc tccgttgact tgcttctgat caaagaggtt
    12901 actctgcgtg taatccgctt cgaggtagaa tgtgtgctgt ttgttatact gtgcactgac
    12961 tcccacgccg ttattccagc cattaccttt gaaactgtac ttctcccggg agttgttcaa
    13021 cagatagtct gtatcccctg aaaactcgcg gatagctcca gtcttcacat acatattcag
    13081 ctggctgttg cctgcggtga tgtcatatcc cagtatcatg ctggcacgcc ccagcagcga
    13141 ttcgtagtga ttcagatgta tattgagacc attactcgcc ttcatagcca tctcattctg
    13201 gtggctgtat gtgagctggg tttgcggctc tatatagaac ccataaccag taggggtcag
    13261 gttgaacctc tgcccggctt ccagggagat gctcaatcca ttcgcagtgc cgttggcgtt
    13321 aacgccgttg ttctgactgt ccagtacgtg aaaactattt ttctggcgcg atgcttttac
    13381 aacgagatcg ctgtaaaaac cgttgtgtgc catgtaactg gcatacattc ccatgtagtc
    13441 tgaacgtgcg gtaccgtcgc ctccgctata gtccggcgat gcatgtgttg agccaatata
    13501 cagaccgaca tacaacggca ttacatcaga gagacgttta tccccaccaa actggatacc
    13561 gctgtaaccc atgtcaaagc cgctcagttt gccactggcg aaggagtcca ggcttccccc
    13621 ataactgcgc agccagatat taccgtcttt actctgattt cgcaggtcac ccattcgttg
    13681 catcagcgta cggttttcaa catagtttaa taagtaaccg acattgagat aattgccgcc
    13741 agcatctgcg gtcgtggtgg gtttaggtgt gggagtgggg ttaggcgtag gatcgggttc
    13801 aggcgtagga tcggggttga ctatgggagg ctgagcggga gccggtgttg gttcaggatt
    13861 aggagtcggt tccggaactg tcccggaagc gtaaagctcc cagttagtgc catttttacg
    13921 cacatcgtac agatatcccc ccaactcaac ctgagaagac gcgctgaacg aggccgcacc
    13981 gtcagtggtt tttaccactg tcagaacttc atttcccgta gtggcctcgc tgccctggtt
    14041 gcggatagcc aatacgtgat taccagcact acttccgctg atattcaata aatccccttt
    14101 attattaacg ccattaccct cgccaacaac atcagcacgc ataataaagg tactgttacc
    14161 gctcaggttc tctacgttta atgtggcaaa tgtgccggca gttgacccgt ggctggcaaa
    14221 atcgacagtt gaatggctca gcgccagcgt gtcgagatta gaattacttg ttacgttcca
    14281 gacgctgtta ttcattgcaa cgtccagttt cccgccattg acattatcgc tgagggagga
    14341 acctgtccaa accgaccctg ggtgcatatc tagattgatt agcccacctt tggaaagaac
    14401 gctaccgttg ataagcatac gacccgaggc gttgatgcgg ctggcggcat aaccatcgtc
    14461 atgttgcgtt gcaatggcca tctggtcggg tgtactcata tcaatgacca gatcgctcgt
    14521 gaggtcgatc tggctgttgg tcatggcata aattcccctg gctcctgccg cgccggtgat
    14581 agccaaactg tctccggtta ttctaccgcc gctgagcgcc cacaaaccca gcgccagact
    14641 gccattacga tcaactgtaa tatcggtatt ttgcatgttg acaacagccg ttgccgtctg
    14701 ggccgaggca ccataagaac cgccggaaaa gatgctgttt cgttgcgctg ccgtgccaga
    14761 aaaattgatt gtcgcgtctg aaccactggt gacgaggcca ccgccctgcg cggaagaaat
    14821 atggctatct gcaccgatag tggttgtacc accacgaact tcgacgccat tggccgcggc
    14881 tccagttaca tcaacagtga gtgcattcgc gctaacctgg ccaaagctcc agaggccgtg
    14941 tgcattatcg ccattggttt taatggaact gtttgttccg agatcgacaa cagagttttt
    15001 ctgtacgttt atccccatgg cgctgtagcc ctgaacatcg attgtcaggt ctgtagccgt
    15061 aaaacgcgca gcaccattgg cgttattgcc gttgagacca ccgatataaa cacctgtact
    15121 tccatcggtc gtgattttac ttccgcttcc aagatcgaca ctggtgccat agtcattgat
    15181 ggttaggcct ataccgttcg agttttcaat ggtgaattga gtggctgtta gtgttgagct
    15241 atgcccaata atgatgccgt catcgttact cttaatggtg ctgcctgtgc ctaagtcagc
    15301 atgggtatag tcaccaatta agttaatgcc gatggcagag gtttgaccaa caacatctac
    15361 tgtgagtcgg ttagctgtca gggagctgtt tttgccctga ataatgattc cttttgcata
    15421 tccagaggcg tcgttgacat tgacggtgac atcattaccc aggttgatat tgccaggcgt
    15481 gtttcctgct ggggtcatta caccatagag gtttcctgat ggatcggcgg tatctcctgt
    15541 aattctgtca ccttcattaa tagtaatatt ggtgccatat tgacttcctg tatcagcagc
    15601 tatcagcagg ggggaaaaga taattgcatt caaaccaata gcgccaaatt ttatcagttg
    15661 aattgagggc gaacggattt ttaattcagc gagacgatct gctttggtat tcccattgtg
    15721 ttgtttgatg cccataccac tcctatatag ta
//
LOCUS       DAZKHF020000092        15746 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2201S31241
            SAMN43267800-rid25106413.denovo.093, whole genome shotgun sequence.
ACCESSION   DAZKHF020000092 DAZKHF020000000
VERSION     DAZKHF020000092.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267800
            Sequence Read Archive: SRR30307263
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 15746)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15746)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 15746)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350581.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350581.2
            Genome Coverage       :: 151x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:21:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,652
            CDSs (total)                      :: 5,557
            Genes (coding)                    :: 5,320
            CDSs (with protein)               :: 5,320
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 237
            CDSs (without protein)            :: 237
            Pseudo Genes (ambiguous residues) :: 0 of 237
            Pseudo Genes (frameshifted)       :: 102 of 237
            Pseudo Genes (incomplete)         :: 130 of 237
            Pseudo Genes (internal stop)      :: 52 of 237
            Pseudo Genes (multiple problems)  :: 41 of 237
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15746
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267800-rid25106413.denovo.093"
                     /serotype="O 26:H11"
                     /isolate="2201S31241"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-01"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            complement(<1..1336)
                     /gene="uidB"
                     /locus_tag="ACHOSC_RS12020"
                     /old_locus_tag="pgaptmp_002404"
     CDS             complement(<1..1336)
                     /gene="uidB"
                     /locus_tag="ACHOSC_RS12020"
                     /old_locus_tag="pgaptmp_002404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucuronide transporter"
                     /protein_id="HGU4548331.1"
                     /translation="MNQQLSWRTIVGYSLGDVANNFAFAMGALFLLSYYTDVAGVGAA
                     AAGTMLLLVRVFDAFADVFAGRVVDSVNTRWGKFRPFLLFGTAPLMIFSVLVFWVPTD
                     WSHGSKVVYAYLTYMGLGLCYSLVNIPYGSLATAMTQQPQSRARLGAARGIAASLTFV
                     CLAFLIGPSIKNSSPEEMVSVYHFWTIVLAIAGMVLYFICFKSTRENVVRIVAQPSLK
                     ISLQTLKRNRPLFMLCIGALCVLISTFAVSASSLFYVRYVLNDTGLFTVLVLVQNLVG
                     TVASAPLVPGMVARIGKKNTFLIGALLGTCGYLLFFWVSVWSLPVALVALAIASIGQG
                     VTMTVMWALEADTVEYGEYLTGVRIEGLTYSLFSFTRKCGQAIGGSIPAFILGLSGYI
                     ANQVQTPEVIMGIRTSIALVPCGFMLLAFVIIWFYPLTDKKFKEIVVEIDNRK"
     gene            complement(1333..3144)
                     /gene="uidA"
                     /locus_tag="ACHOSC_RS12025"
                     /old_locus_tag="pgaptmp_002405"
     CDS             complement(1333..3144)
                     /gene="uidA"
                     /locus_tag="ACHOSC_RS12025"
                     /old_locus_tag="pgaptmp_002405"
                     /EC_number="3.2.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-glucuronidase"
                     /protein_id="HGU4548332.1"
                     /translation="MLRPVETPTREIKKLDGLWAFSLDRENCGIDQRWWESALQESRA
                     IAVPGSFNDQFADADIRNYAGNVWYQREVFIPKGWAGQRIVLRFDAVTHYGKVWVNNQ
                     EVMEHQGGYTPFEADVTPYVIAGKSVRITVCVNNELNWQTIPPGMVITDENGKKKQSY
                     FHDFFNYAGIHRSVMLYTTPNTWVDDITVVTHVAQDCNHASVDWQVVANGDVSVELRD
                     AEQQVVATGQGTSGTLQVVNPHLWQPGEGYLYELCVTAKSQTECDIYPLRVGIRSVAV
                     KGEQFLINHKPFYFTGFGRHEDADLRGKGFDNVLMVHDHALMDWIGANSYRTSHYPYA
                     EEMLDWADEHGIVVIDETAAVGFNLSLGIGFEAGNKPKELYSEEAVNGETQQAHLQAI
                     KELIARDKNHPSVVMWSIANEPDTRPQGAREYFAPLAEATRKLDPTRPITCVNVMFCD
                     AHTDTISDLFDVLCLNRYYGWYVQSGDLETAEKVLEKELLAWQEKLHQPIIITEYGVD
                     TLAGLHSMYTDMWSEEYQCAWLDMYHRVFDRVSAVVGEQVWNFADFATSQGILRVGGN
                     KKGIFTRDRKPKSAAFLLQKRWTGMNFGEKPQQGGKQ"
     gene            complement(3533..4123)
                     /gene="uidR"
                     /locus_tag="ACHOSC_RS12030"
                     /old_locus_tag="pgaptmp_002406"
     CDS             complement(3533..4123)
                     /gene="uidR"
                     /locus_tag="ACHOSC_RS12030"
                     /old_locus_tag="pgaptmp_002406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator UidR"
                     /protein_id="HGU4548333.1"
                     /translation="MMDNMQTEAQPTRTRILNAAREIFSENGFHSASMKAICKSCAIS
                     PGTLYHHFISKEALIQAIILQDQERALARFREPIEGIHFVDYMVESIVSLTHEAFGQR
                     ALVVEIMAEGMRNPQVAAMLKNKHMTITEFVAERMRDAQQKGEISPDINTSMTSRLLL
                     DLTYGVLADIEAEDLAREASFAQGLRAMIGGILTAS"
     gene            complement(4344..5111)
                     /gene="hdhA"
                     /locus_tag="ACHOSC_RS12035"
                     /old_locus_tag="pgaptmp_002407"
     CDS             complement(4344..5111)
                     /gene="hdhA"
                     /locus_tag="ACHOSC_RS12035"
                     /old_locus_tag="pgaptmp_002407"
                     /EC_number="1.1.1.159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="7-alpha-hydroxysteroid dehydrogenase"
                     /protein_id="HGU4548334.1"
                     /translation="MFNSDNLRLDGKCAIITGAGAGIGKEIAITFATAGASVVVSDIN
                     ADAANHVVDEIQQLGGQAFACRCDITSEQELSALADFAISKLGKVDILVNNAGGGGPK
                     PFDMPMADFRRAYELNVFSFFHLSQLVAPEMEKNGGGVILTITSMAAENKNINMTSYA
                     SSKAAASHLVRNMAFDLGEKNIRVNGIAPGAILTDALKSVITPEIEQKMLQHTPIRRL
                     GQPQDIANAALFLCSPAASWVSGQILTVSGGGVQELN"
     gene            complement(5223..6251)
                     /gene="malI"
                     /locus_tag="ACHOSC_RS12040"
                     /old_locus_tag="pgaptmp_002408"
     CDS             complement(5223..6251)
                     /gene="malI"
                     /locus_tag="ACHOSC_RS12040"
                     /old_locus_tag="pgaptmp_002408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mal regulon transcriptional regulator MalI"
                     /protein_id="HGU4548335.1"
                     /translation="MATAKKITIHDVALAAGVSVSTVSLVLSGKGRISTATGERVNAA
                     IEELGFVRNRQASALRGGQSGVIGLIVRDLSAPFYAELTAGLTEALEAQGRMVFLLHG
                     GKDGEQLAQRFSLLLNQGVDGVVIAGAAGSSDDLRRMAEEKAIPVIFASRASYLDDVD
                     TVRPDNMQAAQLLTEHLIRNGHQRIAWLGGQSSSLTRAERVGGYCATLLKFGLPFHSD
                     WVLECTSSQKQAAEAITALLRHNPTISAVVCYNETIAMGAWFGLLKAGRQSGESGVDR
                     YFEQQVSLAAFTDATPTTLDDIPVTWASTPARELGITLADRMMQKITHEETHSRNLII
                     PARLIAAK"
     gene            6426..8018
                     /gene="malX"
                     /locus_tag="ACHOSC_RS12045"
                     /old_locus_tag="pgaptmp_002409"
     CDS             6426..8018
                     /gene="malX"
                     /locus_tag="ACHOSC_RS12045"
                     /old_locus_tag="pgaptmp_002409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS maltose transporter subunit IICB"
                     /protein_id="HGU4548336.1"
                     /translation="MTAKTAPKVTLWEFFQQLGKTFMLPVALLSFCGIMLGIGSSLSS
                     HDVITLIPVLGNPVLQAIFTWMSKIGSFAFSFLPVMFCIAIPLGLARENKGVAAFAGF
                     VGYAVMNLAVNFWLTNKGILPTTDAAVLKANNIQSILGIQSIDTGILGAVIAGIIVWM
                     LHERFHNIRLPDALAFFGGTRFVPIISSLVMGLVGLVIPLVWPIFAMGISGLGHMINS
                     AGDFGPMLFGTGERLLLPFGLHHILVALIRFTDAGGTQEVCGQTVSGALTIFQAQLSC
                     PTTHGFSESATRFLSQGKMPAFLGGLPGAALAMYHCARPENRHKIKGLLISGLIACVV
                     GGTTEPLEFLFLFVAPVLYVIHALLTGLGFTVMSVLGVTIGNTDGNIIDFVVFGILHG
                     LSTKWYMVPVVAAIWFVVYYVIFRFAITRFNLKTPGRDSEVASSIEKAVAGAPGKSGY
                     NVPAILEALGGADNIVSLDNCITRLRLSVKDMSLVNVLALKDNRAIGVVQLNQHNLQV
                     VIGPQVQSVKDEMAGLMHTVQA"
     gene            8028..9200
                     /gene="malY"
                     /locus_tag="ACHOSC_RS12050"
                     /old_locus_tag="pgaptmp_002410"
     CDS             8028..9200
                     /gene="malY"
                     /locus_tag="ACHOSC_RS12050"
                     /old_locus_tag="pgaptmp_002410"
                     /EC_number="4.4.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional maltose regulon transcriptional
                     repressor/cystathionine beta-lyase MalY"
                     /protein_id="HGU4548337.1"
                     /translation="MFDFSKVVDRHGTWCTQWDYVADRFGTADLLPFTISDMDFATAP
                     CIIEALNQRLMHGVFGYSRWKNDEFLAAIAHWFSTQHYTAIDSQTVVYGPSVIYMVSE
                     LIRQWSETGEGVVIHTPAYDAFYKAIEGNQRTVMPVALEKQADGWFCDMGKLEAVLAK
                     PECKIMLLCSPQNPTGKVWTCDELEIMADLCERHGVRVISDEIHMDMVWGEQPHIPWS
                     NVARGDWALLTSGSKSFNIPALTGAYGIIENSSSRDAYLSALKGRDGLSSPSVLALTA
                     HIAAYQQGAPWLDALRVYLKDNLTYISDKMNAAFPELNWQIPQSTYLAWLDLRLLNID
                     DNALQKALIEQEKVAIMPGYTYGEEGRGFVRLNAGCPRSKLEKGVAGLINAIRAVR"
     gene            9304..10305
                     /gene="add"
                     /locus_tag="ACHOSC_RS12055"
                     /old_locus_tag="pgaptmp_002411"
     CDS             9304..10305
                     /gene="add"
                     /locus_tag="ACHOSC_RS12055"
                     /old_locus_tag="pgaptmp_002411"
                     /EC_number="3.5.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004000 - adenosine deaminase activity
                     [Evidence IEA];
                     GO_process: GO:0009168 - purine ribonucleoside
                     monophosphate biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosine deaminase"
                     /protein_id="HGU4548338.1"
                     /translation="MIDTTLPLTDIHRHLDGNIRPQTILELGRQYNISLPAQSLETLI
                     PHVQVIANEPDLVSFLTKLDWGVKVLASLDACRRVAFENIEDAARHGLHYVELRFSPG
                     YMAMAHQLPVAGVVEAVIDGVREGCRTFGVQAKLIGIMSRTFGEAACQQELEAFLAHR
                     DQITALDLAGDELGFPGSLFLSHFNRARDAGWHITVHAGEAAGPESIWQAIRELGAER
                     IGHGVKAIEDRALMDFLAEQQIGIESCLTSNIQTSTVAELAAHPLKTFLEHGIRASIN
                     TDDPGVQGVDIIHEYTVAAPAAGLSREQIRQAQINGLEMAFLSAEEKRALREKVAAK"
     gene            complement(10340..11380)
                     /gene="ydgJ"
                     /locus_tag="ACHOSC_RS12060"
                     /old_locus_tag="pgaptmp_002412"
     CDS             complement(10340..11380)
                     /gene="ydgJ"
                     /locus_tag="ACHOSC_RS12060"
                     /old_locus_tag="pgaptmp_002412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310359.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxidoreductase"
                     /protein_id="HGU4548339.1"
                     /translation="MSDNIRVGLIGYGYASKTFHAPLIAGTPGLELAVISSSDETKVK
                     ADWPTVTVVSEPKHLFNDPNIDLIVIPTPNDTHFPLAKAALEAGKHVVVDKPFTVTLS
                     QARELDALAKSLGRVLSVFHNRRWDSDFLTLKGLLAEGVLGEVAYFESHFDRFRPQVR
                     DRWREQGGPGSGIWYDLAPHLLDQAITLFGLPVSMTVDLAQLRPGAQSTDYFHAILSY
                     PQRRVILHGTMLAAAESARYIVHGSRGSYVKYGLDPQEERLKNGERLPQEDWGYDMRD
                     GVLTRVEGEERVEETLLTVPGNYPAYYAAIRDALNGDGENPVPASQAIQVMELIELGI
                     ESAKHRATLCLA"
     gene            11623..11748
                     /gene="blr"
                     /locus_tag="ACHOSC_RS12065"
                     /old_locus_tag="pgaptmp_002413"
     CDS             11623..11748
                     /gene="blr"
                     /locus_tag="ACHOSC_RS12065"
                     /old_locus_tag="pgaptmp_002413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_025303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="division septum protein Blr"
                     /protein_id="HGU4548340.1"
                     /translation="MNRLIELTGWIVLVVSVILLGVASHIDNYQPPEQSASVQHK"
     gene            12021..12236
                     /gene="ydgT"
                     /locus_tag="ACHOSC_RS12070"
                     /old_locus_tag="pgaptmp_002414"
     CDS             12021..12236
                     /gene="ydgT"
                     /locus_tag="ACHOSC_RS12070"
                     /old_locus_tag="pgaptmp_002414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription modulator YdgT"
                     /protein_id="HGU4548341.1"
                     /translation="MTVQDYLLKFRKISSLESLEKLYDHLNYTLTDDQELINMYRAAD
                     HRRAELVSGGRLFDLGQVPKSVWHYVQ"
     gene            12322..12762
                     /gene="ydgK"
                     /locus_tag="ACHOSC_RS12075"
                     /old_locus_tag="pgaptmp_002415"
     CDS             12322..12762
                     /gene="ydgK"
                     /locus_tag="ACHOSC_RS12075"
                     /old_locus_tag="pgaptmp_002415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707526.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2569 domain-containing protein"
                     /protein_id="HGU4548342.1"
                     /translation="MTTTTPQRIGGWLLGPLAWLLVALLSTTLALLLYTAALSSPQTF
                     QTLGGQALTTQILWGVSFITAIAMWYYTLWLTIAFFKRRRCVPKHYIIWLLISVLLAV
                     KAFAFSPVEDGIAVRQLLFTLLATALIVPYFKRSSRVKATFVNP"
     gene            12839..13420
                     /gene="rsxA"
                     /locus_tag="ACHOSC_RS12080"
                     /old_locus_tag="pgaptmp_002416"
     CDS             12839..13420
                     /gene="rsxA"
                     /locus_tag="ACHOSC_RS12080"
                     /old_locus_tag="pgaptmp_002416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017456621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxA"
                     /protein_id="HGU4548343.1"
                     /translation="MTDYLLLFVGTVLVNNFVLVKFLGLCPFMGVSKKLETAMGMGLA
                     TTFVMTLASICAWLIDTWILIPLNLIYLRTLAFILVIAVVVQFTEMVVRKTSPVLYRL
                     LGIFLPLITTNCAVLGVALLNINLGHNFLQSALYGFSAAIGFSLVMVLFAAIRERLAV
                     ADVPAPFRGHAIALITAGLMSLAFMGFSGLVKL"
     gene            13420..13998
                     /gene="rsxB"
                     /locus_tag="ACHOSC_RS12085"
                     /old_locus_tag="pgaptmp_002417"
     CDS             13420..13998
                     /gene="rsxB"
                     /locus_tag="ACHOSC_RS12085"
                     /old_locus_tag="pgaptmp_002417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxB"
                     /protein_id="HGU4548344.1"
                     /translation="MNAIWIAVAAVSLLGLAFGAILGYASRRFAVEDDPVVEKIDEIL
                     PQSQCGQCGYPGCRPYAEAISCNGEKINRCAPGGEAVMLKIAELLNVEPQPLDGEAQE
                     ITPARMVAVIDENNCIGCTKCIQACPVDAIVGATRAMHTVMSDLCTGCNLCVDPCPTH
                     CISLQPVAETPDSWKWDLNTIPVRIIPVEHHA"
     gene            13991..>15746
                     /gene="rsxC"
                     /locus_tag="ACHOSC_RS12090"
                     /old_locus_tag="pgaptmp_002418"
     CDS             13991..>15746
                     /gene="rsxC"
                     /locus_tag="ACHOSC_RS12090"
                     /old_locus_tag="pgaptmp_002418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxC"
                     /protein_id="HGU4548345.1"
                     /translation="MLKLFSAFRKNKIWDFNGGIHPPEMKTQSNGTPLRQVPLAQRFV
                     IPLKQHIGAEGELCVSVGDKVLRGQPLTRGRGKMLPVHAPTSGTVTAIAPHSTAHPSA
                     LAELSVIIDADGEDCWIPRDGWADYRTRSREELIERIHQFGVAGLGGAGFPTGVKLQG
                     GGDKIETLIINAAECEPYITADDRLMQDCAAQVVEGIRILAHILQPREILIGIEDNKP
                     QAISMLRAVLADSNDISLRVIPTKYPSGGAKQLTYILTGKQVPHGGRSSDIGVLMQNV
                     GTAYAVKRAVIDGEPITERVVTLTGEAIARPGNVWARLGTPVRHLLNDAGFCPSADQM
                     VIMGGPLMGFTLPWLDVPVVKITNCLLAPSANELGEPQEEQSCIRCSACADACPADLL
                     PQQLYWFSKGQQHDKATTHNIADCIECGACAWVCPSNIPLVQYFRQEKAEIAAIRQEE
                     KRAAEAKARFEARQARLEREKAARLERHKSAAVQPAAKDKDAIAAALARVKEKQAQAT
                     QPIVIKAGERPDNSAIIAAREARKAQARAKQAELQQTNDAATVADPRKTAVEAAIARA
                     KARKLEQQQANAEPEEQVD"
BASE COUNT         3752 a         4093 c         3921 g         3980 t
ORIGIN      
        1 ttttacgatt atcaatttca accacgattt ctttgaattt tttatccgtg agcggataaa
       61 accagataat aacgaatgcc agtagcataa atccgcaagg tactaaggca attgatgtgc
      121 ggatgcccat aataacttcc ggcgtttgca cctgattggc gatatatccg cttaatccca
      181 aaataaaggc aggaattgaa cctccgattg cctgaccgca tttacgggta aatgagaata
      241 gtgaataggt gagcccttca attcgcacgc cggtcaggta ttcaccgtat tctacggtat
      301 cagcttccag tgcccacatc acagtcatag taacgccctg accaattgaa gcgatggcca
      361 acgcaaccaa cgccaccggc agtgaccaga cggaaaccca gaagaatagc agataaccgc
      421 aggttcccag caaagcgcca atcaggaagg tattcttttt accgatcctc gcgaccatcc
      481 ccggcaccag cggtgccgat gccacagtac caaccaggtt ttgcaccagt accagcacag
      541 tgaacagccc ggtatcattt aacacatagc gcacgtagaa caacgacgag gcgctgaccg
      601 cgaaggtcga aatcagcaca cacagcgcac cgatgcacaa cataaacagc gggcgattcc
      661 gtttcagggt ttgcagactg atcttcaatg acggctgcgc cacgatacgt accacattct
      721 cacgcgtcga tttgaagcag atgaagtaaa gcaccattcc ggcaatcgcc agcacaatcg
      781 tccagaaatg gtataccgac accatctctt ccgggctgga gttcttaatg ctcggtccta
      841 tcagaaatgc caggcagaca aaggttaatg aagcggcaat cccgcgagcc gcgcccagac
      901 gggcgcggga ttgtggttgt tgggtcatcg cggtagcaag tgaaccataa ggaatattca
      961 ccaggctgta gcaaagcccg aggcccatgt aggtcaaata tgcatacacc actttgctac
     1021 catggctcca gtcggtcggc acccagaata ccagcacgct gaagatcatt aacggcgcag
     1081 taccgaagag taaaaacggg cggaattttc cccagcgggt attcacactg tccaccactc
     1141 gtccggcaaa gacgtcggcg aaggcatcga atacccgcac cagtaacagc atggtgcccg
     1201 ccgcagcggc accgacgcca gcgacgtcgg tgtagtaact caataggaag agcgccccca
     1261 ttgcgaaggc gaagttattg gcgacgtcac cgaggctgta gccgacgatg gtgcgccagg
     1321 agagttgttg attcattgtt tgcctccctg ctgcggtttt tcaccgaagt tcatgccagt
     1381 ccagcgtttt tgcagcagaa aagccgccga cttcggtttg cggtcgcgag tgaagatccc
     1441 tttcttgtta ccgccaacgc gcaatatgcc ttgcgaggtc gcaaaatcgg cgaaattcca
     1501 tacctgttca ccgacgacgg cgctgacgcg atcaaagacg cggtgataca tatccagcca
     1561 tgcacactga tactcttcac tccacatgtc ggtgtacatt gagtgcagcc cggctaacgt
     1621 atccacgccg tattcggtga tgataatcgg ctgatgcagt ttctcctgcc aggccagaag
     1681 ttctttttcc agtaccttct ctgccgtttc caaatcgccg ctttggacat accatccgta
     1741 ataacggttc aggcacagca catcaaagag atcgctgatg gtatcggtgt gagcgtcgca
     1801 gaacattaca ttgacgcagg tgatcggacg cgtcgggtcg agtttacgcg ttgcttccgc
     1861 cagtggcgcg aaatattccc gtgcaccttg cggacgggta tccggttcgt tggcaatact
     1921 ccacatcacc acgcttgggt ggtttttgtc acgcgctatc agctctttaa tcgcctgtaa
     1981 gtgcgcttgc tgagtttccc cgttgactgc ctcttcgctg tacagttctt tcggcttgtt
     2041 gcccgcttcg aaaccaatgc ctaaagagag gttaaagccg acagcagcag tttcatcaat
     2101 caccacgatg ccatgttcat ctgcccagtc gagcatctct tcagcgtaag ggtaatgcga
     2161 ggtacggtag gagttggccc caatccagtc cattaatgcg tggtcgtgca ccatcagcac
     2221 gttatcgaat cctttgccac gcaagtccgc atcttcatga cgaccaaagc cagtaaagta
     2281 gaacggtttg tggttaatca ggaactgttc gcccttcact gccactgacc ggatgccgac
     2341 gcgaagcggg tagatatcac actctgtctg gcttttggct gtgacgcaca gttcatagag
     2401 ataaccttca cccggttgcc agaggtgcgg attcaccact tgcaaagtcc cgctagtgcc
     2461 ttgtccagtt gcaaccacct gttgctccgc atcacgcagt tcaacgctga catcaccatt
     2521 ggccaccacc tgccagtcaa cagacgcgtg gttacagtct tgcgcgacat gcgtcaccac
     2581 ggtgatatcg tccacccagg tgttcggcgt ggtgtagagc attacgctgc gatggatccc
     2641 ggcatagtta aagaaatcat ggaagtaaga ctgctttttc ttgccgtttt cgtcggtaat
     2701 caccattccc ggcgggatag tctgccagtt cagttcgttg ttcacacaaa cggtgatacg
     2761 tacacttttc ccggcaataa catacggcgt gacatcggct tcaaatggcg tatagccgcc
     2821 ctgatgctcc atcacttcct gattattgac ccacactttg ccgtaatgag tgaccgcatc
     2881 gaaacgcagc acgatacgct ggcctgccca acctttcggt ataaagactt cgcgctgata
     2941 ccagacgttg cccgcataat tacgaatatc tgcatcggcg aactgatcgt taaaactgcc
     3001 tggcacagca attgcccggc tttcttgtaa cgcgctttcc caccaacgct gatcaattcc
     3061 acagttttcg cgatccagac tgaatgccca caggccgtcg agttttttga tttcacgggt
     3121 tggggtttct acaggacgta acataaggga ctcctcatta agataaaaat actggtcaac
     3181 ctttaatctg attagttaac caattctgtg ttgcacacag ttatctgtga gaaacccatg
     3241 gaagagaaat ggaaataaaa agaactgaca ccgcgatcaa aaaaccaaaa actgcaatac
     3301 ccacagcgtg atattgatcg cattaatctt taaaacggcg ttgacagaag ccataaaata
     3361 agcaaccgaa cgttcgttcg agcatattgg gaacgataaa ctctcatgac atatggctac
     3421 ggtgaatatt tttggggggg aattctccga tcccggctag attgtatttt gtacagggaa
     3481 attaaagtat gaaacgcggg cacagttatc accagcccgc gaaatgagag gatcaggatg
     3541 cggttaagat accgccaatc atcgcgcgta atccctgagc aaacgacgct tcacgcgcca
     3601 gatcttccgc ttcgatatcg gccagtacac cgtaggtcag atccagcagt aaacgtgaag
     3661 tcattgacgt gttgatgtct gggcttatct cgcctttttg ctgggcatca cgcatccgct
     3721 cggcaacaaa ttcagtgatc gtcatatgct tatttttaag catggcggcg acctgtgggt
     3781 tacgcatccc ttccgccata atttcgacca ccagcgcccg ttgcccaaag gcttcatggg
     3841 tgagagagac aatagactcg accatatagt caacaaaatg aatcccttca atcggttccc
     3901 ggaaacgggc cagcgccctc tcctggtcct gtaagataat cgcctgaatc aaggcttctt
     3961 tggagatgaa atggtgatag agcgtcccgg gactaatggc gcaggattta cagatggctt
     4021 tcatcgaggc actgtgaaat ccattttctg aaaaaatctc tctggcagca ttgaggatcc
     4081 gggtccgtgt cggttgtgct tcagtctgca tgttatccat cataaaacca ttatagttag
     4141 cgtgaataac tagtttaccg cgttgtcagg tgagcgccta tcattgctgt tgaatagtgc
     4201 gaaggcacac tctattcgta tacttttcac tctgaaattg gttaaccaat agccgtgatt
     4261 tggctgccag ttatttagca gagacgttga tgagtttatc ccggtaaaca acacgcacgg
     4321 ttgtttaccg gtccgttagt gtattaattg agctcctgta ccccaccacc ggagacggtg
     4381 agaatttgtc cgcttaccca gctcgcagca ggcgagcaaa ggaacagcgc tgcgttagca
     4441 atatcttgcg gttggcccag acgtctgatc ggcgtgtgct gtaacatttt ttgttcaatt
     4501 tctggtgtaa taacggattt cagggcatcg gttaatattg cccccggcgc aatgccattt
     4561 acccgaatat ttttttcacc caggtcaaac gccatatttc tgaccagatg actggctgca
     4621 gctttagatg atgcatagga agtcatgttt atatttttat tttctgccgc catagaagtg
     4681 atggtcagaa taacgccacc gccatttttt tccatttctg gcgcaacaag ttgtgacaga
     4741 tggaaaaaag aaaacacatt cagttcataa gcacggcgaa aatccgccat tggcatatca
     4801 aacggtttag gtccaccgcc accggcgttg ttaaccagaa tatcaacttt acccagctta
     4861 ctgatagcaa agtctgccag tgcagagagt tcctgttcgg aagtaatatc acaacggcag
     4921 gcaaatgcct gaccacccag ttgttgaatt tcgtctacaa catggttagc tgcgtcggcg
     4981 ttaatatcac tgaccaccac agatgcgcca gctgtcgcga atgtaatggc gatttcttta
     5041 ccaatacctg cacccgcacc tgtgatgatg gcgcattttc cgtcgagtct caggttgtca
     5101 gaattaaaca cataaacctc ctgtgaactt cagtaaaaat aaaaggcacc gcctgtgtgc
     5161 cacatttttt atcattaacc ttgaggaaag gatactacca ggtatagcag ataaagagag
     5221 gattatttcg ccgcaatgag ccgggcggga ataataagat tgcgtgaatg cgtctcttca
     5281 tgggtgattt tttgcatcat gcgatccgca agtgtgatac caagttcccg cgctggcgtg
     5341 ctggcccagg taacaggtat atcatcaagt gtggttggtg tcgcatcggt aaatgccgcc
     5401 agcgaaacct gttgctcaaa gtaacgatcg actccgcttt ccccgctttg ccgccctgct
     5461 ttgagcaaac caaaccatgc ccccatcgca atagtttcgt tatagcaaac cacggcactg
     5521 atggtcgggt tatgacgtaa aagcgccgtg atagcttccg cggcttgctt ctggctggaa
     5581 gtgcactcca acacccaatc gctatgaaac ggcaggccaa attttagtag agttgcacaa
     5641 tagcccccca cccgttctgc acgggttaat gaggaacttt gccctcccag ccaggcgatc
     5701 cgctgatgcc cattgcgaat gagatgctcc gtcaacaact gtgcagcctg catgttgtcc
     5761 gggcgaaccg tatcaacatc atcaagataa ctggcacggg aagcgaaaat caccgggata
     5821 gctttttctt ctgccatccg tcgcaggtca tcgctgcttc ctgcagcccc ggcaattacc
     5881 acaccatcca caccctgatt cagtaacagt gaaaaccgct gtgccagctg ctcgccgtct
     5941 ttaccgccgt gaagcaaaaa aaccatccgt ccctgcgctt ccagagcttc cgtcaatccg
     6001 gccgtcaatt cggcgtaaaa cggcgcagat aaatcacgga cgatcaaacc aatgacgccg
     6061 ctttgcccgc cgcgcagcgc cgacgcctgg cgattgcgca caaatcccag ctcttcaatg
     6121 gcggcgttaa cgcgttctcc tgtggcggta gagattcgcc ctttgccact aagcaccagc
     6181 gaaacggtac ttaccgacac gcccgcagcc agcgcaacat catgaatggt tatttttttg
     6241 gcggtagcca tgcagatgac ctactccctg attatgtgac agataaaacg ttttaccttt
     6301 tatttatctt atactcgcta ttatcgttgc gtaatgtgat ttatgcctca ctaaaatttg
     6361 ataaaacgtt ttatcttctc gcgcaattta ctgaatccag attgttctct acgaggagtc
     6421 gttttatgac ggcgaaaaca gcaccgaaag tcacgctgtg ggagttcttc cagcagttag
     6481 gcaaaacctt catgttaccc gtggcattat tgtcgttctg cggcattatg ctcggcattg
     6541 gtagttctct tagtagccac gatgtcatca ccctgatccc ggtcctgggc aaccccgtgt
     6601 tgcaggctat ctttacctgg atgagtaaga ttggctcgtt tgcttttagt ttcctgccag
     6661 tcatgttctg tatcgccatc ccgctgggtc tggcacgcga aaacaaaggc gtagcggcat
     6721 tcgctggctt cgtcggttat gcggtaatga acctcgcggt aaacttctgg ttgaccaata
     6781 aaggcattct gccaaccacg gacgccgcgg ttctgaaagc caataacatc cagagcattc
     6841 ttgggatcca gtcgatcgac accggtatcc tcggtgcggt gatcgccggt attatcgtct
     6901 ggatgctgca tgagcgattc cataatatcc gcctgccgga tgcgcttgca ttcttcggcg
     6961 gtacgcgctt cgtaccaatc atctcctcgc tggtgatggg ccttgtcggc ctggtgattc
     7021 cattagtctg gccgattttc gcgatgggta ttagcggctt gggccatatg ataaacagcg
     7081 cgggtgattt cggaccgatg ctgtttggta ccggtgaacg tctgctgttg ccgtttggtc
     7141 tgcatcacat tctggtggca ttaattcgct ttaccgacgc aggcggcacg caggaagtct
     7201 gcggtcaaac cgtcagcggc gcattgacca tcttccaggc gcaattgagt tgcccgacca
     7261 ctcacggttt ttctgaaagc gccacgcgtt tcctttcgca aggtaaaatg cctgcgtttc
     7321 tcggcggtct gccaggtgca gcgttagcta tgtatcactg cgcgcgcccg gaaaatcgcc
     7381 ataaaattaa aggtctgctg atttctggcc tgatcgcctg cgtcgttggc ggcactaccg
     7441 aaccgctgga attcctgttc ctgttcgtag cgccagttct gtatgtcatc cacgcgctgt
     7501 taaccggcct cggcttcacc gtcatgtctg tgctcggcgt caccatcggt aataccgacg
     7561 gcaatatcat cgacttcgtg gtgttcggta ttttgcatgg tctgtcaacc aagtggtaca
     7621 tggtgccagt ggtggcggca atctggtttg tcgtttacta cgtcatcttc cgtttcgcta
     7681 tcacccgctt caatctgaaa accccggggc gcgatagcga agttgccagc tcaatcgaaa
     7741 aagccgttgc cggtgcgccg ggtaaatcag gttacaacgt tcctgcaatc ctcgaagcat
     7801 taggcggtgc cgacaatatt gtcagcctcg ataactgcat tacccgtctg cgtttgtctg
     7861 tgaaagatat gtcgcttgtt aatgtgctgg cactgaagga caatcgggca attggcgtag
     7921 tacaacttaa tcaacataac ctgcaggttg ttatcgggcc acaagttcag tcagtaaaag
     7981 atgaaatggc cggtctgatg catactgtcc aggcataagg ataagatatg ttcgattttt
     8041 caaaggtcgt ggatcgtcat ggcacatggt gtacacagtg ggattatgtc gctgaccgtt
     8101 tcggcactgc tgacctgtta ccgttcacga tttcagacat ggattttgcc actgccccct
     8161 gcattatcga ggcgctgaat cagcgcctga tgcacggcgt atttggctac agccgctgga
     8221 aaaacgatga gtttctcgcg gctattgccc actggttttc cacccagcat tacaccgcca
     8281 tcgattctca gacggtggtg tatggccctt ctgtcatcta tatggtttca gaactgattc
     8341 gtcagtggtc tgaaacaggt gaaggcgtgg tgatccacac acccgcctat gacgcatttt
     8401 acaaggccat tgaaggtaac cagcgcacag taatgcccgt tgctttagag aagcaggctg
     8461 atggttggtt ttgcgatatg ggcaagttgg aagccgtgtt ggcgaaacca gaatgtaaaa
     8521 ttatgctcct gtgtagccca cagaatccta ccgggaaagt gtggacgtgc gatgagctgg
     8581 agatcatggc tgacctatgc gagcgtcatg gtgtgcgggt tatttccgat gaaatccata
     8641 tggatatggt ctggggcgag cagccgcata ttccctggag taatgtggcg cggggagact
     8701 gggcgttgct gacgtcgggc tcgaaaagtt tcaatattcc cgcgctgacc ggtgcttacg
     8761 ggattataga aaatagcagc agccgtgatg cctatttatc ggcactgaaa ggacgtgatg
     8821 ggctttcttc cccttcggta ctggcgttaa ctgcccatat cgccgcctat cagcaaggtg
     8881 caccgtggct ggatgcctta cgcgtctatc tgaaagataa cctgacgtat atctcagata
     8941 aaatgaacgc cgcgtttcct gaactcaact ggcagatccc gcaatccact tatctggcct
     9001 ggcttgatct acgtctgttg aatattgacg acaacgcgtt gcaaaaagcg cttatcgagc
     9061 aagaaaaagt cgcgatcatg ccggggtata cctatggtga agaaggtcgt ggttttgtcc
     9121 gtctcaatgc cggctgccca cgttcgaaac tggaaaaagg tgtggctgga ttaataaacg
     9181 ccatccgcgc tgttcgctaa ccccaattgc gcaacgtaaa aaatcgttgc gcaatcgtgg
     9241 atttttaccc tgctttgttt ttataatggt gcgcactttt atattcagaa aaagagtgcg
     9301 accatgattg ataccaccct gccattaact gatatccatc gccaccttga tggcaacatt
     9361 cgtccccaga ccattcttga acttggccgc cagtataata tctcgcttcc tgcacaatcc
     9421 ctggaaacac tgattcccca cgttcaggtc attgccaacg aacccgatct ggtgagcttt
     9481 ctgaccaaac ttgactgggg cgttaaagtt ctcgcctctc ttgatgcctg tcgccgcgtg
     9541 gcatttgaaa acattgaaga tgcagcccgt cacggcctgc actatgtcga gctgcgtttt
     9601 tcaccaggct acatggcaat ggcacatcag ctgcctgtag cgggtgttgt cgaagcggtg
     9661 atcgatggcg tacgtgaagg ttgccgcacc tttggtgtgc aggcgaagct tatcggcatt
     9721 atgagccgga ccttcggcga agccgcctgt cagcaagagc tggaggcctt tttagcccac
     9781 cgtgaccaga ttaccgcact tgatttagcc ggtgatgaac ttggtttccc gggaagtctg
     9841 ttcctttctc acttcaaccg cgcgcgtgat gcgggctggc atattaccgt ccatgcaggc
     9901 gaagctgccg ggccggaaag catctggcag gcgattcgtg aactgggtgc ggagcgtatt
     9961 ggacatggcg taaaagccat tgaagatcgg gcgctgatgg attttctcgc cgagcaacaa
    10021 attggtattg aatcctgtct gacctccaat attcagacca gcaccgtagc agagctggct
    10081 gcacatccgc tgaaaacgtt ccttgagcat ggcattcgtg ccagcattaa cactgacgat
    10141 cccggcgtac agggagtgga tatcattcac gaatataccg ttgccgcgcc agctgctggg
    10201 ttatcccgcg agcaaatccg ccaggcacag attaatggtc tggaaatggc tttcctcagc
    10261 gctgaggaaa aacgcgcact gcgagaaaaa gtcgccgcga agtaacaaaa tggatggtgc
    10321 aaattgcacc atccattttt catgcaaggc acaaagtcgc gcgatgtttg gcggattcga
    10381 tgcccagctc aatcaactcc attacctgga ttgcctggct tgccggaacc ggattttcac
    10441 catcgccatt taacgcatca cgaatagccg catagtaagc cggataattc ccaggtaccg
    10501 tcaacagcgt ttcttcgaca cgttcctcac cttccacgcg ggtaagtacg ccatcacgca
    10561 tatcgtagcc ccagtcttcc tgcggcagac gctcgccatt tttcagacgt tcttcctgtg
    10621 gatcgaggcc atatttcaca taactgcctc gggatccatg cacgatatac cgtgctgact
    10681 cagcggctgc cagcatggta ccgtgtaaaa tgactcgccg ctgtggatag gacaagatgg
    10741 cgtggaaata atcggtcgac tgcgctccgg gccgtaactg tgccaaatct accgtcatgc
    10801 tgaccggtaa accaaaaagc gtaatggcct gatcaagaag atgtggtgct aaatcgtacc
    10861 agataccgct gcctgggccg ccctgttcac gccaacgatc gcgcacctgc ggacggaagc
    10921 ggtcaaaatg agactcaaag taagcaactt cacccagcac gccttccgcg agtaaacctt
    10981 ttagcgtcaa gaaatcgcta tcccagcgac ggttatggaa tacagacagc acacgcccca
    11041 ggctttttgc cagcgcatcc agctctcgcg cttgtgacag tgtcacggta aagggtttat
    11101 caacgaccac atgtttaccc gcctcaagcg ccgctttggc taacgggaaa tgggtatcgt
    11161 tgggtgtagg aatgacaatc aggtctatgt tgggatcgtt aaacagatgc ttcggctcag
    11221 agacaaccgt aaccgttggc cagtcggctt ttacttttgt ttcatcactg ctggagatta
    11281 ccgccagttc cagcccgggc gtgcccgcaa tcaggggcgc atggaaggtt ttgctcgcat
    11341 aaccataccc aatcaaccca acacggatgt tgtcgctcat gtcattgcct ctcattgaag
    11401 tatgatggct atttgacact atcctttacc cacgctcaac agtttaataa cctgccagca
    11461 ataagggatg ttgtttaact taagtcaaaa aaatagcgaa ttttccaacg acaaaagcta
    11521 aatatcgcaa aaacctcagt aaaaatcttg ctggagctat tattgctaag taacatttac
    11581 cccctgaagt taatggatca atcaagagag atgtgggctg taatgaatcg tcttattgaa
    11641 ttaacaggtt ggatcgttct tgtcgtttca gtcattcttc ttggcgtggc gagtcacatt
    11701 gacaactatc agccacctga acagagtgct tcggtacaac acaagtaagc tctgcacttg
    11761 tggagcgaca tgctgcccgt ccgggtgcat gttttcactt gtcggatatt aaaccaggaa
    11821 tttattatct tgttcgatgt tgttggtgat tgtcagggat agtaaagcgc gttactcttt
    11881 tcagagcagt ttttcatccc tgaatatttg ccatcagcgc attcttatta ttgtaataac
    11941 acaagcgttc acttttggtg aaattttatc atcactaata atacctaccc ctttggtatt
    12001 aattatttta tggacctttt atgactgttc aggactactt attaaaattt cgcaaaatca
    12061 gttcactcga aagtctggaa aaactctacg accatcttaa ttacaccctg acggacgatc
    12121 aggaactgat caatatgtat cgtgctgccg atcaccgtcg cgcagagctg gtttctggcg
    12181 ggcgtttgtt tgacctcggc caggtaccga agtccgtctg gcactatgtc caataaagaa
    12241 agtagcgatc ggttataaaa gccttataat aacgccccta aaattagatt tgcctgtttg
    12301 cgtcgctatt ctggagaacc tatgaccacc acaacgccac aacgtattgg aggctggttg
    12361 cttggcccgt tggcctggct tttagtcgct ctgttgagta cgacgctggc gttgttgctg
    12421 tataccgctg cgttatcttc tccgcaaaca tttcaaacgc tcggcggaca agcactgacg
    12481 acacaaatct tatggggcgt ttcttttatt accgctatcg ccatgtggta ttacacactg
    12541 tggttgacca tcgctttctt taaacgtcgg cgttgtgttc ctaaacacta tattatctgg
    12601 ctgcttattt ccgtattgct ggcagtaaaa gcattcgctt tttcacccgt ggaagatggc
    12661 atagccgttc gccagttgct gtttactttg ttggcaaccg cattgatcgt cccctatttc
    12721 aaacgctcgt cgcgggtaaa agcgacgttt gtgaatccgt aataacctta cagttaacct
    12781 gttgtcgcct gctctggatt aacggataat aggcggcttt tttatttcag gccgaaaaat
    12841 gactgactac ctgttactgt ttgtcggaac tgtactggtc aataactttg tactggtcaa
    12901 gtttctcggt ctctgtccgt ttatgggggt ttccaaaaaa ctggaaaccg cgatgggcat
    12961 ggggctggca acaacgtttg tgatgacgct ggcgtctatt tgcgcctggc ttatcgatac
    13021 gtggattttg atcccactta atctgattta cctgcgcacc ctggcattta ttctggtgat
    13081 tgctgtggtc gtgcagttca ccgagatggt ggtgcgcaaa accagcccgg tgctttaccg
    13141 tctgctgggg atttttttgc cgcttatcac caccaactgt gcagtgctcg gcgtggcgtt
    13201 gctgaatatc aatctcgggc acaatttctt gcagtcggcg ctgtacggtt tttccgccgc
    13261 tatcggtttc tcgctggtga tggtgctctt cgccgccatc cgcgaacgcc ttgctgtggc
    13321 tgatgtcccg gcaccttttc gcggtcatgc cattgcgtta attaccgcag gtcttatgtc
    13381 tctggccttt atgggcttta gtggtttggt gaagttgtaa tgaatgctat ctggattgcc
    13441 gttgccgccg tgagcctgct gggcctggcg tttggcgcca ttctgggtta tgcctcccgc
    13501 cgttttgcgg tggaagacga tccggtcgtt gagaaaattg acgaaatctt accgcagagc
    13561 cagtgtggtc agtgcggtta tcccggctgt cgcccctacg cggaagccat cagctgtaac
    13621 ggtgaaaaaa tcaaccgttg cgccccaggt ggcgaagctg tgatgctaaa aattgccgag
    13681 ttgcttaatg tcgagccgca gccgctggat ggcgaagcgc aagagataac gcctgcgcgg
    13741 atggtggcgg ttattgatga aaataactgt attggctgca ctaaatgtat tcaggcgtgt
    13801 ccggtagacg ccatcgttgg cgctacccgt gccatgcata cggtaatgag tgatctctgt
    13861 acgggctgca atttatgtgt tgatccgtgc ccgacgcact gcatctcgtt gcaaccggtc
    13921 gcagaaacac ctgactcctg gaaatgggat ctgaacacca ttcccgtgcg tatcattccc
    13981 gtggaacacc atgcttaagt tattctctgc attcagaaaa aataaaatct gggatttcaa
    14041 cggcggcatc catccaccgg agatgaaaac ccagtccaac ggtacacccc tgcgccaggt
    14101 accactggcg cagcgttttg ttattccact gaaacagcat attggcgctg aaggtgagtt
    14161 gtgcgttagc gtcggcgata aagtattgcg cggccagccg cttacccgtg gtcgcggcaa
    14221 aatgctgcct gttcacgcgc ccacctcggg taccgttacg gctattgcgc cccactctac
    14281 ggctcatcct tcagctttag ctgaattaag cgtgattatt gatgccgatg gtgaagactg
    14341 ctggatcccg cgcgacggct gggccgatta tcgcactcgc agtcgcgaag agttaatcga
    14401 gcgcatacat cagtttggtg ttgccgggct gggcggtgca ggattcccga caggcgttaa
    14461 attgcagggt ggcggagata agattgaaac gttgattatc aacgcggctg agtgcgagcc
    14521 gtacattacc gccgatgacc gtttgatgca ggattgcgcg gctcaggtcg tagagggtat
    14581 tcgcattctt gcgcatattc tgcagccacg cgaaattctt atcggcattg aagataacaa
    14641 accgcaggcg atttccatgc tgcgcgcggt gctggcggac tctaacgata tttctctgcg
    14701 ggtgattcca accaaatatc cttctggcgg tgctaaacaa ttaacctaca ttctgaccgg
    14761 gaagcaggtt ccacatggcg ggcgttcatc cgatatcggc gtattaatgc aaaacgtcgg
    14821 cactgcttat gcagtgaaac gtgccgttat tgatggcgag ccgattaccg agcgtgttgt
    14881 aaccctgact ggcgaagcaa tcgctcgccc gggcaacgtc tgggcacggc tggggacgcc
    14941 agtgcgtcat ttattgaatg atgccggatt ctgcccctct gccgatcaaa tggtgattat
    15001 gggtggcccg ctaatgggct ttaccttgcc atggctggat gtcccggtcg taaagattac
    15061 caactgtctg ttggctccct ctgccaatga acttggcgaa ccacaggaag aacaaagctg
    15121 catccggtgt agcgcctgtg ctgacgcctg ccctgcggat cttttgccgc aacagttgta
    15181 ctggttcagc aaaggtcagc aacacgataa agctaccacg cataacattg ctgattgcat
    15241 tgaatgtggg gcttgcgcgt gggtttgccc gagcaatatt cccctggtgc aatatttccg
    15301 tcaggaaaaa gctgaaattg cggctattcg tcaggaagaa aagcgcgccg cagaagccaa
    15361 agcgcgtttc gaagcgcgcc aggctcgtct ggagcgcgaa aaagcggctc gccttgaacg
    15421 acataagagc gcagccgttc aacctgcagc caaagataaa gatgcgattg ctgccgctct
    15481 ggcgcgggtg aaagagaaac aggcccaggc tacacagcct attgtgatta aagcgggcga
    15541 acgcccggat aacagtgcaa ttattgcagc acgggaagcc cgtaaagcgc aagccagagc
    15601 gaaacaggca gaactgcagc aaactaacga cgcagcaacc gttgctgatc cacgtaaaac
    15661 tgccgttgaa gcagctatcg cccgcgccaa agcgcgcaag ctggaacagc aacaggctaa
    15721 tgcggaacca gaagaacagg tcgatc
//
LOCUS       DAZKHF020000093        15712 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2201S31241
            SAMN43267800-rid25106413.denovo.094, whole genome shotgun sequence.
ACCESSION   DAZKHF020000093 DAZKHF020000000
VERSION     DAZKHF020000093.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267800
            Sequence Read Archive: SRR30307263
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 15712)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15712)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 15712)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350581.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350581.2
            Genome Coverage       :: 151x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:21:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,652
            CDSs (total)                      :: 5,557
            Genes (coding)                    :: 5,320
            CDSs (with protein)               :: 5,320
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 237
            CDSs (without protein)            :: 237
            Pseudo Genes (ambiguous residues) :: 0 of 237
            Pseudo Genes (frameshifted)       :: 102 of 237
            Pseudo Genes (incomplete)         :: 130 of 237
            Pseudo Genes (internal stop)      :: 52 of 237
            Pseudo Genes (multiple problems)  :: 41 of 237
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15712
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267800-rid25106413.denovo.094"
                     /serotype="O 26:H11"
                     /isolate="2201S31241"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-01"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            262..666
                     /gene="sdhC"
                     /locus_tag="ACHOSC_RS12095"
                     /old_locus_tag="pgaptmp_002419"
                     /gene_synonym="cybA"
                     /gene_synonym="dhsC"
     CDS             262..666
                     /gene="sdhC"
                     /locus_tag="ACHOSC_RS12095"
                     /old_locus_tag="pgaptmp_002419"
                     /gene_synonym="cybA"
                     /gene_synonym="dhsC"
                     /EC_number="1.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000263334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0045273 - respiratory chain complex II
                     (succinate dehydrogenase) [Evidence IEA];
                     GO_function: GO:0000104 - succinate dehydrogenase activity
                     [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase cytochrome b556 subunit"
                     /protein_id="HGU4548346.1"
                     /translation="MWALFMIRNVKKQRPVNLDLQTIRFPITAIASILHRVSGVITFI
                     AVGILLWLLGTSLSSPEGFEQASAIMGSFFVKFIMWGILTALAYHVVVGIRHMMMDFG
                     YLEETFEAGKRSAKISFVITVVLSLLAGVLVW"
     gene            660..1007
                     /gene="sdhD"
                     /locus_tag="ACHOSC_RS12100"
                     /old_locus_tag="pgaptmp_002420"
                     /gene_synonym="cybS"
                     /gene_synonym="dhsD"
     CDS             660..1007
                     /gene="sdhD"
                     /locus_tag="ACHOSC_RS12100"
                     /old_locus_tag="pgaptmp_002420"
                     /gene_synonym="cybS"
                     /gene_synonym="dhsD"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase membrane anchor subunit"
                     /protein_id="HGU4548347.1"
                     /translation="MVSNASALGRNGVHDFILVRATAIVLTLYIIYMVGFFATSGELT
                     YEVWIGFFASAFTKVFTLLALFSILIHAWIGMWQVLTDYVKPLALRLMLQLVIVVALV
                     VYVIYGFVVVWGV"
     gene            1007..2773
                     /gene="sdhA"
                     /locus_tag="ACHOSC_RS12105"
                     /old_locus_tag="pgaptmp_002421"
     CDS             1007..2773
                     /gene="sdhA"
                     /locus_tag="ACHOSC_RS12105"
                     /old_locus_tag="pgaptmp_002421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005122549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase flavoprotein subunit"
                     /protein_id="HGU4548348.1"
                     /translation="MKLPVREFDAVVIGAGGAGMRAALQISQSGQTCALLSKVFPTRS
                     HTVSAQGGITVALGNTHEDNWEWHMYDTVKGSDYIGDQDAIEYMCKTGPEAILELEHM
                     GLPFSRLDDGRIYQRPFGGQSKNFGGEQAARTAAAADRTGHALLHTLYQQNLKNHTTI
                     FSEWYALDLVKNQDGAVVGCTALCIETGEVVYFKARATVLATGGAGRIYQSTTNAHIN
                     TGDGVGMAIRAGVPVQDMEMWQFHPTGIAGAGVLVTEGCRGEGGYLLNKHGERFMERY
                     APNAKDLAGRDVVARSIMIEIREGRGCDGPWGPHAKLKLDHLGKEVLESRLPGILELS
                     RTFAHVDPVKEPIPVIPTCHYMMGGIPTKVTGQALTVNEKGEDVVVPGLFAVGEIACV
                     SVHGANRLGGNSLLDLVVFGRAAGLHLQESIAEQGALRDASESDVEVSLDRLNRWNNN
                     RNGEDPVAIRKALQECMQHNFSVFREGDAMAKGLEQLKVIRERLKNARLDDTSSEFNT
                     QRVECLELDNLMETAYATAVSANFRTESRGAHSRFDFPDRDDENWLCHSLYLPESESM
                     TRRSVNMEPKLRPAFPPKIRTY"
     gene            2789..3505
                     /gene="sdhB"
                     /locus_tag="ACHOSC_RS12110"
                     /old_locus_tag="pgaptmp_002422"
     CDS             2789..3505
                     /gene="sdhB"
                     /locus_tag="ACHOSC_RS12110"
                     /old_locus_tag="pgaptmp_002422"
                     /EC_number="1.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012133276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase iron-sulfur subunit
                     SdhB"
                     /protein_id="HGU4548349.1"
                     /translation="MRLEFSIYRYNPDVDDAPRMQDYTLEAEEGRDMMLLDALIQLKE
                     KDPSLSFRRSCREGVCGSDGLNMNGKNGLACITPISALNQPGKKIVIRPLPGLPVIRD
                     LVVDMGQFYAQYDKIKPYLLNNGQNPPAREHLQMPEQREKLDGLYECILCACCSTSCP
                     SFWWNPDKFIGPAGLLAAYRFLIDSRDTETDSRLDGLSDAFSVFRCHSIMNCVSVCPK
                     GLNPTRAIGHIKSMLLQRNA"
     gene            3806..6607
                     /gene="sucA"
                     /locus_tag="ACHOSC_RS12115"
                     /old_locus_tag="pgaptmp_002423"
     CDS             3806..6607
                     /gene="sucA"
                     /locus_tag="ACHOSC_RS12115"
                     /old_locus_tag="pgaptmp_002423"
                     /EC_number="1.2.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045252 - oxoglutarate dehydrogenase
                     complex [Evidence IEA];
                     GO_function: GO:0004591 - oxoglutarate dehydrogenase
                     (succinyl-transferring) activity [Evidence IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoglutarate dehydrogenase E1 component"
                     /protein_id="HGU4548350.1"
                     /translation="MQNSALKAWLDSSYLSGANQSWIEQLYEDFLTDPDSVDANWRST
                     FQQLPGTGVKPDQFHSQTREYFRRLAKDASRYSSTISDPDTNVKQVKVLQLINAYRFR
                     GHQHANLDPLGLWQQDKVADLDPSFHDLTEADFQETFNVGSFASGKETMKLGELLEAL
                     KQTYCGPIGAEYMHITSTEEKRWIQQRIESGRATFNSEEKKRFLSELTAAEGLERYLG
                     AKFPGAKRFSLEGGDALIPMLKEMIRHAGNSGTREVVLGMAHRGRLNVLVNVLGKKPQ
                     DLFDEFAGKHKEHLGTGDVKYHMGFSSDFQTDGGLVHLALAFNPSHLEIVSPVVIGSV
                     RARLDRLDEPSSNKVLPITIHGDAAVTGQGVVQETLNMSKARGYEVGGTVRIVINNQV
                     GFTTSNPLDARSTPYCTDIGKMVQAPIFHVNADDPEAVAFVTRLALDFRNTFKRDVFI
                     DLVCYRRHGHNEADEPSATQPLMYQKIKKHPTPRKIYADKLEQEKVATLEDATEMVNL
                     YRDALDAGDCVVAEWRPMNMHSFTWSPYLNHEWDEEYPNKVEMKRLQELAKRISTVPE
                     AVEMQSRVAKIYGDRQAMAAGEKLFDWGGAENLAYATLVDEGIPVRLSGEDSGRGTFF
                     HRHAVIHNQSNGSTYTPLQHIHNGQGAFRVWDSVLSEEAVLAFEYGYATAEPRTLTIW
                     EAQFGDFANGAQVVIDQFISSGEQKWGRMCGLVMLLPHGYEGQGPEHSSARLERYLQL
                     CAEQNMQVCVPSTPAQVYHMLRRQALRGMRRPLVVMSPKSLLRHPLAVSSLEELANGT
                     FLPAIGEIDELDPKGVKRVVMCSGKVYYDLLEQRRKNNQHDVAIVRIEQLYPFPHKAM
                     QEVLQQFAHVKDFVWCQEEPLNQGAWYCSQHHFREVIPFGASLRYAGRPASASPAVGY
                     MSVHQKQQQDLVNDALNVE"
     gene            6622..7839
                     /gene="odhB"
                     /locus_tag="ACHOSC_RS12120"
                     /old_locus_tag="pgaptmp_002424"
     CDS             6622..7839
                     /gene="odhB"
                     /locus_tag="ACHOSC_RS12120"
                     /old_locus_tag="pgaptmp_002424"
                     /EC_number="2.3.1.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045252 - oxoglutarate dehydrogenase
                     complex [Evidence IEA];
                     GO_function: GO:0004149 - dihydrolipoyllysine-residue
                     succinyltransferase activity [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoglutarate dehydrogenase complex
                     dihydrolipoyllysine-residue succinyltransferase"
                     /protein_id="HGU4548351.1"
                     /translation="MSSVDILVPDLPESVADATVATWHKKPGDAVVRDEVLVEIETDK
                     VVLEVPASADGILDAVLEDEGTTVTSRQILGRLREGNSAGKETSAKSEEKASTPAQRQ
                     QASLEEQNNDALSPAIRRLLAEHNLDASAIKGTGVGGRLTREDVEKHLAKAPAKESAP
                     AAAAPAAQPALAARSEKRVPMTRLRKRVAERLLEAKNSTAMLTTFNEVNMKPIMDLRK
                     QYGEAFEKRHGIRLGFMSFYVKAVVEALKRYPEVNASIDGDDVVYHNYFDVSMAVSTP
                     RGLVTPVLRDVDTLGMADIEKKIKELAVKGRDGKLTVEDLTGGNFTITNGGVFGSLMS
                     TPIINPPQSAILGMHAIKDRPMAVNGQVEILPMMYLALSYDHRLIDGRESVGFLVTIK
                     ELLEDPTRLLLDV"
     gene            7933..9099
                     /gene="sucC"
                     /locus_tag="ACHOSC_RS12125"
                     /old_locus_tag="pgaptmp_002425"
     CDS             7933..9099
                     /gene="sucC"
                     /locus_tag="ACHOSC_RS12125"
                     /old_locus_tag="pgaptmp_002425"
                     /EC_number="6.2.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012133271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-forming succinate--CoA ligase subunit beta"
                     /protein_id="HGU4548352.1"
                     /translation="MNLHEYQAKQLFARYGLPAPVGYACTTPREAEEAASKIGAGPWV
                     VKCQVHAGGRGKAGGVKVVNSKEDIRAFAENWLGKRLVTYQTDANGQPVNQILVEAAT
                     DIAKELYLGAVVDRSSRRVVFMASTEGGVEIEKVAEETPHLIHKVALDPLTGPMPYQG
                     RELAFKLGLEGKLVQQFTKIFMGLATIFLERDLALIEINPLVITKQGDLICLDGKLGA
                     DGNALFRQPDLREMRDQSQEDPREAQAAQWELNYVALDGNIGCMVNGAGLAMGTMDIV
                     KLHGGEPANFLDVGGGATKERVTEAFKIILSDDKVKAVLVNIFGGIVRCDLIADGIIG
                     AVAEVGVNVPVVVRLEGNNAELGAKKLADSGLNIIAAKGLTDAAQQVVAAVEGK"
     gene            9099..9968
                     /gene="sucD"
                     /locus_tag="ACHOSC_RS12130"
                     /old_locus_tag="pgaptmp_002426"
     CDS             9099..9968
                     /gene="sucD"
                     /locus_tag="ACHOSC_RS12130"
                     /old_locus_tag="pgaptmp_002426"
                     /EC_number="6.2.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinate--CoA ligase subunit alpha"
                     /protein_id="HGU4548353.1"
                     /translation="MSILIDKNTKVICQGFTGSQGTFHSEQAIAYGTKMVGGVTPGKG
                     GTTHLGLPVFNTVREAVAATGATASVIYVPAPFCKDSILEAIDAGIKLIITITEGIPT
                     LDMLTVKVKLDEAGVRMIGPNCPGVITPGECKIGIQPGHIHKPGKVGIVSRSGTLTYE
                     AVKQTTDYGFGQSTCVGIGGDPLPGSNFIDILEMFEKDPQTEAIVMIGEIGGSAEEEA
                     AAYIKEHVTKPVVGYIAGVTAPKGKRMGHAGAIIAGGKGTADEKFAALEAAGVKTVRS
                     LADIGEALKTVLK"
     gene            complement(10072..10794)
                     /gene="mngR"
                     /locus_tag="ACHOSC_RS12135"
                     /old_locus_tag="pgaptmp_002427"
     CDS             complement(10072..10794)
                     /gene="mngR"
                     /locus_tag="ACHOSC_RS12135"
                     /old_locus_tag="pgaptmp_002427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannosyl-D-glycerate transport/metabolism system
                     transcriptional repressor MngR"
                     /protein_id="HGU4548354.1"
                     /translation="MGHKPLYRQIADRIREQIARGELKPGDALPTESALQTEFGVSRV
                     TVRQALRQLVEQQILESIQGSGTYVKEERVNYDIFQLTSFDEKLSDRHVDTHSEVLIF
                     EVIPADDFLQQQLQITAQDRVWHVKRVRYRKQKPMALEETWMPLALFPDLTWQVMENS
                     KYHFIEEVKKMVIDRSEQEIIPLMPTEEMSRLLNISQTKPILEKVSRGYLVDGRVFEY
                     SRNAFNTDDYKFTLIAQRKSSR"
     gene            10975..12891
                     /gene="mngA"
                     /locus_tag="ACHOSC_RS12140"
                     /old_locus_tag="pgaptmp_002428"
     CDS             10975..12891
                     /gene="mngA"
                     /locus_tag="ACHOSC_RS12140"
                     /old_locus_tag="pgaptmp_002428"
                     /EC_number="2.7.1.195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001393949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005351 - carbohydrate:proton symporter
                     activity [Evidence IEA];
                     GO_function: GO:0022877 -
                     protein-N(PI)-phosphohistidine-fructose phosphotransferase
                     system transporter activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS 2-O-a-mannosyl-D-glycerate transporter
                     subunit IIABC"
                     /protein_id="HGU4548355.1"
                     /translation="MNLTTLTHRDALCLNARFTSREEAIHALTQRLAALGKISSTEQF
                     LEEVYRRESLGPTALGEGLAVPHGKTAAVKEAAFAVATLSEPLQWEGVDGPEAVDLVV
                     LLAIPPNEAGTTHMQLLTALTTRLADDEIRARIQSATTPDELLSALDDKGGTQPSASF
                     SNAPTIVCVTACPAGIAHTYMAAEYLEKAGRKLGVNVYVEKQGANGIEGRLTADQLNS
                     ATACIFAAEVAIKESERFNGIPALSVPVAEPIRHAEALIQQALTLKRSDETRTVQQDT
                     QPVKSVKTELKQALLSGISFAVPLIVAGGTVLAVAVLLSQIFGLQDLFNEENSWLWMY
                     RKLGGGLLGILMVPVLAAYTAYSLADKPALAPGFAAGLAANMIGSGFLGAVVGGLIAG
                     YLMRWVKNHLRLGSKFNGFLTFYLYPVLGTLGAGSLMLFVVGEPVAWINNSLTAWLNG
                     LSGSNALLLGAILGFMCSFDLGGPVNKAAYAFCLGAMANGVYGPYAIFASVKMVSAFT
                     VTASTMLAPRLFKEFEIETGKSTWLLGLAGITEGAIPMAIEDPLRVIGSFVLGSMVTG
                     AIVGAMNIGLSTPGAGIFSLFLLHDNGAGGVMAAIGWFGAALVGAAISTAILLMWRRH
                     AVKHGNYLTDGVMP"
     gene            12909..15542
                     /gene="mngB"
                     /locus_tag="ACHOSC_RS12145"
                     /old_locus_tag="pgaptmp_002429"
     CDS             12909..15542
                     /gene="mngB"
                     /locus_tag="ACHOSC_RS12145"
                     /old_locus_tag="pgaptmp_002429"
                     /EC_number="3.2.1.170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004559 - alpha-mannosidase activity
                     [Evidence IEA];
                     GO_process: GO:0006013 - mannose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannosylglycerate hydrolase"
                     /protein_id="HGU4548356.1"
                     /translation="MKAVSRVHITPHMHWDREWYFTTEESRILLVNNMEEILCRLEQD
                     NEYKYYVLDGQTAILEDYFAVKPENKDRVKKQVEAGKLIIGPWYTQTDTTIVSAESIV
                     RNLMYGMRDCLAFGEPMKIGYLPDSFGMSGQLPHIYNGFGITRTMFWRGCSERHGTDK
                     TEFLWQSSDGSEVTAQVLPLGYAIGKYLPADEDGLRKRLDSYFDVLEKASVTKEILLP
                     NGHDQMPLQQNIFEVMDKLREIYPQRKFVMSRFEEVFEKIEAQRDNLATLKGEFIDGK
                     YMRVHRTIGSTRMDIKIAHARIENKIVNLLEPLATLAWTLGFEYHHGLLEKMWKEILK
                     NHAHDSIGCCCSDKVHREIVARFELAEDMADNLIRFYMRKIADNMPQSDADKLVLFNL
                     MPWPREEVINTTVRLRASQFNLRDDRGQPVPYFIRHAREIDPGLIDRQIVHYGNYDPF
                     MEFDIQINQIVPSMGYRTLYIEANQPGNVIAAKSDAEGILENAFWQIALNEDGSLQLV
                     DKDSGVRYDRVLQIEESSDDGDEYDYSPAKEEWVITAANAKPQCDIIHEAWQSRAVIR
                     YEMAVPRNLQERSARQSTGRVGVEMVVTLSHNSRRIDVDINLDNQADDHRLRVLIPTP
                     FNTDSVLADTQFGSLTRPVNDSAMNNWQQEGWKEAPVPVWNMLNYVALQEGRNGMAVF
                     SEGLREFEVIGEEKKTFAITLLRGVGLLGKEDLLLRPGRPSGIKMPVPDSQLRGLLSC
                     RLSLLSYTGTPTAAGVAQQARAWLTPVQCYNKIPWDAMKLNKAGFNVPESYSLLKMPP
                     VGCLISALKKAEDRQEVILRLFNPAESATCDATVAFSREVISCSETMMDEHITTEENQ
                     GSNLSGPFLPGQSRTFSYRLA"
BASE COUNT         3757 a         3957 c         4339 g         3659 t
ORIGIN      
        1 caaactatat gtaggttaat tgtaatgatt ttgtgaacag cctatactgc cgccaggtct
       61 ccggaacacc ctgcaatccc gagccaccca gcgttgtaac gtgtcgtttt cgcatctgga
      121 agcagtgttt tgcatgacgc gcagttatag aaaggacgct gtctgacccg caagcagacc
      181 ggaggaagga aatcccgacg tctccaggta acagaaagtt aacctctgtg cccgtagtcc
      241 ccagggaata ataagaacag catgtgggcg ttattcatga taagaaatgt gaaaaaacaa
      301 agacctgtta atctggacct acagaccatc cggttcccca tcacggcgat agcgtccatt
      361 ctccatcgcg tttccggtgt gatcaccttt attgcagtgg gtatcctgct gtggcttctg
      421 ggtaccagcc tctcttcccc tgaaggtttc gagcaagctt ccgcgattat gggcagcttc
      481 ttcgtcaaat ttatcatgtg gggcatcctt accgctctgg cgtatcacgt cgtcgtaggt
      541 attcgccaca tgatgatgga ttttggctat ctggaagaaa cattcgaagc gggtaaacgc
      601 tccgccaaaa tctcctttgt tattactgtc gtgctttcac ttctcgcagg agtcctcgta
      661 tggtaagcaa cgcctccgca ttaggacgca atggcgtaca tgatttcatc ctcgttcgcg
      721 ctaccgctat cgtcctgacg ctctacatca tttatatggt cggttttttc gccaccagtg
      781 gcgaactgac atatgaagtc tggatcggtt tcttcgcctc tgcgttcacc aaagtgttca
      841 ccctgctggc gctgttttct atcttgattc atgcctggat cggcatgtgg caggtgttga
      901 ccgactacgt taaaccgctg gcattgcgcc tgatgctgca actggtgatt gtcgttgcac
      961 tggtggttta cgtgatttat ggattcgttg tggtgtgggg tgtgtgatga aattgccagt
     1021 cagagaattt gatgcagttg tgattggtgc cggtggcgca ggtatgcgcg cggcgctgca
     1081 aatttcccag agcggccaga cctgtgcgct gctctctaaa gtcttcccga cccgttccca
     1141 taccgtttct gcgcaaggcg gcattaccgt tgcgctgggt aatacccatg aagataactg
     1201 ggaatggcat atgtacgaca ccgtaaaagg gtcggactat atcggtgacc aggacgcgat
     1261 tgaatatatg tgtaaaaccg ggccggaagc gattctggaa ctcgaacaca tgggcctgcc
     1321 gttctcgcgt cttgatgatg gtcgtatcta tcaacgtccg tttggcggtc agtcgaaaaa
     1381 cttcggcggc gagcaggcgg cacgcactgc ggcggcggct gaccgtaccg gtcacgcact
     1441 gttgcacacg ctttatcagc agaacctgaa aaaccacacc accattttct ccgagtggta
     1501 tgcgctggat ctggtgaaaa accaggatgg cgcggtggtg ggttgtaccg cactgtgcat
     1561 cgaaaccggt gaagtggttt atttcaaagc ccgcgctacc gtgctggcga ctggcggggc
     1621 agggcgtatt tatcagtcca ccactaacgc ccacattaac actggcgacg gtgtcggcat
     1681 ggctatccgc gcaggcgtac cggtgcagga tatggaaatg tggcaattcc acccgaccgg
     1741 cattgccggt gcgggcgtac tggtcaccga aggttgccgt ggtgaaggcg gttatctgct
     1801 gaacaaacat ggcgaacgct ttatggagcg ttatgcgccg aacgccaaag acctggcggg
     1861 ccgtgatgtg gtggcgcgtt ccatcatgat tgaaatccgt gaaggtcgcg gctgtgatgg
     1921 tccgtggggg ccacacgcga aactgaaact cgatcacctg ggtaaagaag ttctcgaatc
     1981 ccgtctgccg ggtatcctgg agctttcccg taccttcgct cacgtcgatc cggtgaaaga
     2041 gccgattccg gttatcccaa cctgtcacta catgatgggc ggtattccga ccaaagttac
     2101 cggtcaggcg ctgactgtga atgagaaagg cgaagatgtg gttgttccgg gactgtttgc
     2161 cgttggtgaa atcgcttgtg tatcggtaca cggcgctaac cgtctgggcg gcaactcgct
     2221 gctggacctg gtggtctttg gtcgcgcggc aggtctgcat ctgcaagagt ctatcgccga
     2281 gcagggcgca ctgcgcgatg ccagcgagtc tgatgttgaa gtgtctctgg atcgcctgaa
     2341 ccgctggaac aataatcgta acggtgaaga tccggtggcg attcgtaaag cgctgcaaga
     2401 atgtatgcag cataacttct cggtcttccg tgaaggtgat gcgatggcga aagggcttga
     2461 gcagttgaaa gtgatccgcg agcgtctgaa aaatgcccgt ctggatgaca cttccagcga
     2521 gttcaacacc cagcgcgttg agtgcctgga actggataac ctgatggaaa cggcgtatgc
     2581 aacggctgtt tctgccaact tccgtaccga aagccgtggc gcacatagcc gcttcgactt
     2641 cccggatcgt gatgatgaaa actggctgtg ccactccctg tatctgccag agtcggaatc
     2701 catgacgcgc cgaagcgtca acatggaacc gaaactgcgc ccggcattcc cgccgaagat
     2761 tcgtacttac taatgcggag acaggaaaat gagactcgag ttttcaattt atcgctataa
     2821 cccggatgtt gatgatgctc cgcgtatgca ggattacacc ctggaagcgg aagaaggtcg
     2881 cgacatgatg ctgctggatg cgcttatcca gctgaaagag aaagatccca gcctgtcgtt
     2941 ccgccgctcc tgccgtgaag gtgtgtgcgg ttccgacggt ctgaacatga acggcaagaa
     3001 tggtctggcc tgtattaccc cgatttcggc actcaaccag ccgggcaaga agattgtgat
     3061 tcgcccgctg ccaggtttac cggtgatccg cgatttggtg gtagacatgg gacaattcta
     3121 tgcgcaatat gacaaaatta agccttacct gttgaataat ggacaaaatc cgccagctcg
     3181 cgagcattta cagatgccag agcagcgcga aaaactcgac gggctgtatg aatgtattct
     3241 ctgcgcatgt tgttcaacct cttgtccgtc tttctggtgg aatcccgata agtttatcgg
     3301 cccggcaggc ttgttagcgg catatcgttt cctgatcgat agccgtgata ccgagactga
     3361 cagccgcctc gacggtttga gcgatgcatt cagcgtattc cgctgtcaca gcatcatgaa
     3421 ctgcgtcagt gtatgtccga aggggctgaa cccgacgcgc gccatcggcc atattaagtc
     3481 gatgttgttg caacgtaatg cgtaaaccgt aggcctgata agacgcgcaa gcgtcgcatc
     3541 aggcaaccag tgccggatgc ggcgtgaacg ccttatccgg cctacaagtc attacccgta
     3601 ggcctgataa gcgcagcgca tcaggcgtaa caaagaaatg caggaaatct ttaaaaactg
     3661 cccctgacac taagacagtt tttaaaggtt ccttcgcgag ccactacata gacaagagct
     3721 cgcaagtgaa ccccggcacg cacatcactg tgcgtggtag tatccacggc gaagtaagca
     3781 taaaaaagat gcttaaggga tcacgatgca gaacagcgct ttgaaagcct ggttggactc
     3841 ttcttacctc tctggcgcaa accagagctg gatagaacag ctctatgaag acttcttaac
     3901 cgatcctgac tcggttgacg ctaactggcg ttcgacgttc cagcagttac ctggtacggg
     3961 agtcaaaccg gatcaattcc actctcaaac gcgtgaatat ttccgccgcc tggcgaaaga
     4021 cgcttcacgt tactcttcaa cgatctccga ccctgacacc aatgtgaagc aggttaaagt
     4081 cctgcagctc attaacgcat accgcttccg tggtcaccag catgcgaatc tcgatccgct
     4141 gggactgtgg cagcaagata aagtggccga tctggatccg tctttccacg atctgaccga
     4201 agcagacttc caggagacct tcaacgtcgg ttcatttgcc agcggcaaag aaaccatgaa
     4261 actcggcgag ctgctggaag ccctcaagca aacctactgt ggcccgattg gtgccgagta
     4321 tatgcacatt accagcaccg aagaaaaacg ctggatccaa cagcgtatcg agtctggtcg
     4381 cgcgactttc aatagcgaag agaaaaaacg cttcttaagc gaactgaccg ccgctgaagg
     4441 ccttgaacgt tacctcggcg caaaattccc tggcgcaaaa cgcttctcgc tggaaggcgg
     4501 tgacgcgtta atcccgatgc ttaaagagat gatccgccac gctggcaaca gcggcacccg
     4561 cgaagtggtt cttgggatgg cgcaccgtgg tcgtctgaac gtgctggtga acgtgctggg
     4621 taaaaaaccg caagacttgt tcgacgagtt tgccggtaaa cataaagaac acctcggcac
     4681 gggtgacgtg aaataccaca tgggcttctc gtctgacttc cagaccgatg gcggcctggt
     4741 gcacctggcg ctggcgttta acccgtctca ccttgagatt gtaagcccgg tagttatcgg
     4801 ttctgttcgt gcccgtctgg acagacttga tgagccgagc agcaacaaag tgctgccaat
     4861 caccatccac ggtgacgccg cagtgaccgg gcagggcgtg gttcaggaaa ctctgaacat
     4921 gtcgaaagcg cgtggttatg aagttggcgg tacggtacgt atcgttatca acaaccaggt
     4981 tggtttcacc acctctaatc cgctggatgc tcgttctaca ccgtactgta ctgatatcgg
     5041 taagatggtt caggcaccga ttttccacgt taacgcggat gatccggaag ccgttgcctt
     5101 tgtgacccgt ctggcgctcg atttccgtaa cacctttaaa cgtgatgtct tcatcgacct
     5161 ggtgtgctac cgccgtcacg gccacaacga agccgacgag ccgagcgcaa cccagccgct
     5221 gatgtatcag aaaatcaaaa aacatccgac accgcgcaaa atctacgctg acaagctgga
     5281 gcaggaaaaa gtggcgacgc tggaagatgc caccgagatg gttaacctgt accgcgatgc
     5341 gctggatgct ggcgattgcg tagtggcaga gtggcgtccg atgaacatgc actctttcac
     5401 ctggtcgccg tacctcaacc acgaatggga cgaagagtac ccgaacaaag ttgagatgaa
     5461 gcgcctgcag gagctggcga aacgcatcag cacggtgccg gaagcagttg aaatgcagtc
     5521 tcgcgttgcc aagatttatg gcgatcgcca ggcgatggct gccggtgaga aactgttcga
     5581 ctggggcggt gcggaaaacc tcgcttacgc cacgctggtt gacgaaggca ttccggttcg
     5641 cctgtcgggt gaagactccg gtcgcggtac cttcttccac cgccacgcgg tgatccacaa
     5701 ccagtctaac ggttccactt acacgccgtt gcaacacatc cataacgggc agggcgcgtt
     5761 ccgcgtctgg gactccgtac tgtctgaaga agcagtgctg gcgtttgaat acggttatgc
     5821 caccgcagaa ccacgcactc tgaccatctg ggaagcgcag ttcggtgact tcgccaacgg
     5881 tgcgcaggtg gttatcgacc agttcatctc ttctggcgaa cagaaatggg gccggatgtg
     5941 tggtctggtg atgttgctgc cgcacggtta cgaagggcag gggccggagc actcctccgc
     6001 gcgtctggaa cgttatctgc aactttgtgc tgagcaaaac atgcaggtgt gcgtaccgtc
     6061 taccccggca caggtttacc acatgctgcg tcgtcaggcg ctgcgcggga tgcgtcgtcc
     6121 gctggtcgtg atgtcgccga aatccctgct gcgtcatccg ctggcggttt ccagcctcga
     6181 agaactggcg aacggcacct tcctgccagc catcggtgaa atcgacgagc ttgatccgaa
     6241 gggcgtgaag cgcgtagtga tgtgttctgg taaggtttat tacgacctgc tggaacagcg
     6301 tcgtaagaac aatcaacacg atgtcgccat tgtgcgtatc gagcaactct acccgttccc
     6361 gcataaagcg atgcaggaag tgttgcagca gtttgctcac gtcaaggatt ttgtctggtg
     6421 ccaggaagag ccgctcaacc agggcgcatg gtactgcagc cagcatcatt tccgtgaagt
     6481 gattccgttt ggggcttctc tgcgttatgc aggccgcccg gcctccgcct ctccggcggt
     6541 agggtatatg tccgttcacc agaaacagca acaagatctg gttaatgacg cgctgaacgt
     6601 cgaataaata aaggatacac aatgagtagc gtagatattc tggtccctga cctgcctgaa
     6661 tccgtagccg atgccaccgt cgcaacctgg cataaaaaac ccggcgacgc agtcgtacgt
     6721 gatgaagtgc tggtagaaat cgaaactgac aaagtggtac tggaagtacc ggcatcagca
     6781 gacggcattc tggatgcggt tctggaagat gaaggtacaa cggtaacgtc tcgtcagatc
     6841 cttggtcgcc tgcgtgaagg caacagcgcc ggtaaagaaa ccagcgccaa atctgaagag
     6901 aaagcgtcca ctccggcgca acgccagcag gcatctctgg aagagcaaaa caacgatgcg
     6961 ttaagcccgg cgatccgtcg cctgctggct gaacacaatc tcgacgccag cgccattaaa
     7021 ggcaccggtg tgggtggtcg tctgactcgt gaagatgtgg aaaaacatct ggcgaaagcc
     7081 ccggcgaaag agtctgctcc ggcagcggct gctccggcgg cgcaaccggc tctggctgca
     7141 cgtagtgaaa aacgtgtccc gatgactcgc ctgcgtaagc gtgtggcaga gcgtctgctg
     7201 gaagcgaaaa actccaccgc catgctgacc acgttcaacg aagtcaacat gaagccgatt
     7261 atggatctgc gtaagcagta cggtgaagcg tttgaaaaac gccacggcat ccgtctgggc
     7321 tttatgtcct tctacgtgaa agcggtggtt gaagccctga aacgttaccc ggaagtgaac
     7381 gcgtctatcg acggcgatga cgtggtttac cacaactatt tcgacgtcag catggcggtt
     7441 tctacgccgc gcggcctggt gacaccggta ctgcgtgatg tcgataccct cggcatggca
     7501 gacatcgaga agaaaatcaa agagctggca gtcaaaggcc gtgacggcaa gctgaccgtt
     7561 gaagatctga ccggtggtaa cttcaccatc accaacggtg gtgtgttcgg ttccctgatg
     7621 tctacgccga tcatcaaccc gccgcagagc gcaattctgg gtatgcacgc tatcaaagat
     7681 cgtccgatgg cggtgaatgg tcaggttgag atcctgccga tgatgtacct ggcgctgtcc
     7741 tacgatcacc gtctgatcga tggtcgcgaa tccgtgggct tcctggtaac gatcaaagag
     7801 ttgctggaag atccgacgcg tctgctgctg gacgtgtagt agtttaagct tcacctgcac
     7861 tgtagaccga catggatagg gtgtcggtct acggtttaaa agataacgat tactgaagga
     7921 tggacagaac acatgaactt acatgaatat caggcaaaac aactttttgc ccgctatggc
     7981 ttaccagcac cggtgggtta tgcctgtact actccgcgcg aagcagaaga agccgcttca
     8041 aaaatcggtg ccggcccgtg ggtagtgaaa tgtcaggttc acgctggtgg ccgcggtaaa
     8101 gcgggcggtg tgaaagttgt aaacagcaaa gaagacatcc gtgcttttgc agaaaactgg
     8161 ctcggcaagc gtctggtaac gtatcaaaca gatgccaatg gccaaccggt taaccagatt
     8221 ctggttgaag cagcgaccga tatcgctaaa gagctgtatc tcggtgccgt tgtagaccgt
     8281 agttcccgtc gtgtggtctt tatggcctcc accgaaggcg gcgtggaaat cgaaaaagtg
     8341 gcggaagaaa ctccgcacct gatccacaaa gttgcgcttg atccgctgac tggcccgatg
     8401 ccgtatcagg gacgcgagct ggcgttcaaa ctgggtctgg aaggtaaact ggttcagcag
     8461 ttcaccaaaa tcttcatggg cctggcgacc attttcctgg agcgcgacct ggcgttgatc
     8521 gaaatcaacc cgctggtcat caccaaacag ggcgatctga tttgcctcga cggcaaactg
     8581 ggcgctgacg gcaacgcact gttccgccag cctgatctgc gcgaaatgcg tgaccagtcg
     8641 caggaagatc cgcgtgaagc acaggctgca cagtgggaac tgaactacgt tgcgctggac
     8701 ggtaacatcg gttgtatggt taacggcgca ggtctggcga tgggtacgat ggacatcgtt
     8761 aaactgcacg gcggcgaacc ggctaacttc cttgacgttg gcggcggcgc aaccaaagaa
     8821 cgtgtaaccg aagcgttcaa aatcatcctc tctgacgaca aagtgaaagc cgttctggtt
     8881 aacatcttcg gcggtatcgt tcgttgcgac ctgatcgctg acggtatcat cggcgcggta
     8941 gcagaagtgg gtgttaacgt accggtcgtg gtacgtctgg aaggtaacaa cgccgaactc
     9001 ggcgcgaaga aactggctga cagcggcctg aatattattg cagcaaaagg tctgacggat
     9061 gcagctcagc aggttgttgc cgcagtggag gggaaataat gtccatttta attgataaaa
     9121 acaccaaggt tatctgccag ggctttactg gtagccaggg gactttccac tcagaacagg
     9181 ccattgcata cggcactaaa atggttggcg gcgtaacccc aggtaaaggc ggtactaccc
     9241 acctcggcct gccggtgttc aacaccgtgc gtgaagctgt tgctgctact ggcgctaccg
     9301 cttctgttat ctacgtacca gcaccgttct gcaaagactc cattctggaa gctattgatg
     9361 caggcatcaa actgattatc accatcactg aaggcatccc gacgctggat atgctgactg
     9421 tgaaagtgaa actggacgaa gccggcgttc gtatgatcgg cccgaactgc ccgggcgtta
     9481 tcactccggg tgaatgcaaa atcggtatcc agccgggtca cattcacaaa ccgggtaaag
     9541 tgggtatcgt ttcccgttcc ggtacactga cctatgaagc ggttaaacag accacggatt
     9601 acggtttcgg tcagtcgacc tgcgttggta tcggcggtga cccgctccct ggctctaact
     9661 ttatcgacat tctcgaaatg ttcgaaaaag atccgcagac cgaagcgatc gtaatgatcg
     9721 gtgagatcgg cggtagcgct gaagaagaag cggcagcgta catcaaagag catgttacca
     9781 agccagttgt gggttacatc gctggtgtga ctgcgccgaa aggcaaacgt atgggccacg
     9841 cgggtgccat cattgccggt gggaaaggga ctgcggatga gaaattcgct gctctggaag
     9901 ccgcaggcgt gaaaaccgtt cgcagcctgg cggatatcgg tgaagcactg aaaactgttc
     9961 tgaaataaat atctgtaata agaaatagcc ctcgccgctt ccctctacag gaatggcgaa
    10021 gggctgtcgg tttcgacatg gttggccatc gtatgatggc cttttttgtg cttatcgcga
    10081 tgattttcgt tgcgctatca gggtaaattt atagtcatcg gtattaaaag cgttgcggct
    10141 atattcaaac acccgaccat caactaaata tccacgcgat actttttcaa gaatcggctt
    10201 tgtctggctg atattaagca gacggctcat ctcttcggtt ggcatcagag gaatgatttc
    10261 ctgttcgcta cgatcgataa ccattttctt cacttcttca ataaagtgat attttgaatt
    10321 ttccatgacc tgccaggtga gatccgggaa caacgcaagc ggcatccagg tttcttccag
    10381 cgccattggc ttttgcttgc gatagcgcac gcgcttcaca tgccacacac gatcctgcgc
    10441 ggtgatttgt agctgttgct gaagaaaatc gtcagccgga atcacttcaa atatcaggac
    10501 ttcactgtgt gtatcgacgt gacggtccga cagtttttca tcaaaactgg ttaactgaaa
    10561 aatatcgtaa ttgacccgct cttctttgac gtaagtcccg ctgccctgaa tgctttcgag
    10621 gatctgttgc tcgactaact ggcgcaaagc ctgacgcacc gtaacccggc tgacgccaaa
    10681 ctctgtttgt aatgccgatt cggtaggtaa cgcatcgcca ggtttaagct cgccacgcgc
    10741 aatttgttca cgaatgcgat cggcaatctg ccggtataag ggcttgtgtc ccatttttag
    10801 tatctcatta atacgaattt agccattatg cccgataaat tcatcctgta aataatacaa
    10861 atacaaatac aaatacaata caaataattt caatcaagtg aaattgatca cataatggta
    10921 ttgttttatc gggcacactg gcgcgactat aaaaacgatc aagtgaggat catgatgaat
    10981 ctgacgactc tgacccaccg cgatgcgttg tgtctgaatg cgcgctttac cagccgtgaa
    11041 gaggccatcc acgcgttgac tcaacgtctt gctgctctgg ggaaaatttc cagtactgag
    11101 caatttctgg aagaagtgta tcgccgtgaa agccttggcc cgaccgcctt aggtgaaggg
    11161 ttggctgtgc cgcatggcaa aactgctgcg gtaaaagaag cggcgtttgc ggtcgccaca
    11221 ctcagcgagc cgcttcagtg ggaaggcgtt gatggcccgg aagcggttga tttagtggtg
    11281 ctgctggcga ttccccccaa tgaagcgggt actacgcata tgcaactgct gacagcgctg
    11341 accacgcgcc tggcggatga tgagattcgg gcgcgtatac agtcggcgac gacgcctgat
    11401 gagttgctct cggcgctgga tgacaaggga ggcacgcaac cttctgcctc tttttccaac
    11461 gcgccaacta tcgtctgcgt aacggcctgt ccggcgggta ttgctcacac ctatatggct
    11521 gcggaatatc tggaaaaagc cggacgcaaa ctcggcgtaa atgtttacgt tgaaaaacaa
    11581 ggcgctaacg gcattgaagg gcgtttaacg gcggatcaac tcaatagtgc aaccgcctgt
    11641 atttttgcgg ctgaagtcgc catcaaggag agtgagcgtt ttaatggcat tcccgcgctt
    11701 tcagtgcctg ttgccgagcc gattcgccat gcagaagcgt tgatccaaca agcgcttacc
    11761 ctcaagcgta gcgatgagac gcgtaccgta cagcaagata cgcaaccggt gaaaagtgtc
    11821 aaaacggagc tgaaacaggc actgttgagc ggaatctctt ttgccgtacc gttgattgtc
    11881 gcggggggca cggtgctggc ggtcgcggta ttactgtcgc aaatcttcgg gctacaagat
    11941 ctgtttaatg aagaaaactc ctggctgtgg atgtaccgca agctgggcgg cgggctgctc
    12001 ggaattttga tggtaccggt gctcgcggcc tataccgcct attctctggc agataaaccg
    12061 gcgttagcgc caggctttgc ggctggactt gccgccaaca tgatcggctc cgggtttctc
    12121 ggcgcggtcg ttggcggatt gatagccggt tacttgatgc gctgggtgaa aaatcacttg
    12181 cgtcttggca gtaaattcaa tggcttcctg actttttatc tctacccggt gctcggtacg
    12241 ttgggagcgg gcagtctgat gctgtttgtg gtgggagaac ctgtcgcatg gatcaataac
    12301 tcgcttaccg cctggctgaa cggtctgtca ggaagtaacg cgctgttgct gggtgccatt
    12361 ctcggtttta tgtgttcctt tgaccttgga gggccagtga ataaagccgc ttatgcattc
    12421 tgcctgggcg caatggcgaa cggcgtttac ggcccgtatg ccattttcgc ctccgtcaaa
    12481 atggtttcgg catttaccgt aaccgcttcc acgatgctcg caccgcgcct gtttaaagag
    12541 tttgaaattg agaccgggaa atccacctgg ctgttagggc tggcaggtat taccgaaggg
    12601 gcgatcccga tggcgattga agatccgctg cgggttattg gttcgtttgt gctgggctct
    12661 atggtaacgg gcgctattgt cggtgcgatg aatatcggcc tttcgacacc cggtgccggc
    12721 attttctcgc tctttttact tcatgataat ggcgcgggcg gtgttatggc ggcaattggc
    12781 tggtttggtg cggcattggt gggggctgca atctcgactg caattctcct gatgtggcgg
    12841 cgtcacgcgg ttaagcatgg caactatctg actgatggcg taatgccata aacaaaaagg
    12901 aaacgacgat gaaagcggta tctcgcgttc acatcacccc gcatatgcac tgggatcggg
    12961 agtggtattt caccaccgaa gagtcacgca ttctgctggt caataatatg gaagagatcc
    13021 tgtgccgact ggaacaggac aacgaataca aatattacgt actcgacggg caaacggcga
    13081 tcctcgaaga ttatttcgcg gtgaaaccgg aaaacaaaga ccgtgtgaag aaacaggtag
    13141 aagccggcaa gttgattatc ggcccctggt atacccagac cgataccacg attgtttctg
    13201 cggaatccat cgtccgtaat ctgatgtacg gaatgcgtga ctgcctcgcg tttggcgagc
    13261 cgatgaaaat aggttattta ccggattcct ttggcatgtc cgggcagctt ccgcatatct
    13321 acaatggatt tggcattacc cgcaccatgt tctggcgtgg atgttctgag cgccacggta
    13381 ctgataaaac cgagtttttg tggcaaagca gtgacggtag cgaagtgacg gcgcaagtgt
    13441 tgccgctggg ctacgccatc ggtaagtact tacctgccga cgaagacgga ttacgcaagc
    13501 gcctcgacag ttattttgat gtgctggaaa aagcgtcagt aaccaaagaa attttattgc
    13561 cgaatggtca tgaccagatg ccattgcagc aaaatatctt cgaagtgatg gataagctac
    13621 gtgagatcta ccctcaacgt aagtttgtga tgagccgctt tgaagaggta tttgagaaga
    13681 tcgaagcgca gcgagataat ctggcaaccc tgaaagggga atttattgat ggcaaatata
    13741 tgcgcgtgca tcgcaccatc ggttctacgc gtatggatat caaaattgcc cacgcgcgta
    13801 ttgaaaataa gattgttaat ctgctggaac cgctggcaac actggcctgg acgttgggtt
    13861 ttgaatacca ccacggcttg ctggagaaaa tgtggaaaga gatcttaaaa aatcatgccc
    13921 acgacagtat cggttgttgt tgcagtgaca aagttcatcg cgaaatcgtc gcccgcttcg
    13981 aactggctga agacatggcg gataatctga ttcgtttcta catgcgcaaa attgccgaca
    14041 acatgccgca gagcgacgcc gacaaactcg tcctgtttaa cctgatgccc tggccgcgtg
    14101 aagaagtgat caacaccact gtgcgcctgc gcgccagcca gtttaatttg cgggacgatc
    14161 gcggccagcc tgtgccgtat tttattcgcc atgcccgtga gatcgatcca ggcctaatcg
    14221 atcggcaaat tgttcattat ggcaattatg atccctttat ggagttcgac atccagatca
    14281 accagattgt cccttctatg ggctatcgca cgctttatat tgaagcgaat cagcctggca
    14341 acgtgattgc ggcaaaaagt gacgctgaag ggattctgga aaatgctttc tggcagattg
    14401 cgctcaatga ggatggttct ctgcaactgg tagataaaga cagcggtgtg cgctatgacc
    14461 gggtattgca aattgaagaa agctctgatg atggtgatga atatgactat tcacccgcaa
    14521 aagaagagtg ggtaattacc gcggcgaacg cgaaaccgca atgcgatatt attcatgaag
    14581 cctggcagag cagggctgtt atccgttatg aaatggcggt gccgcgtaat ctgcaagaac
    14641 gcagcgcaag acaatccact ggcagagtag gggtggagat ggttgtcact ctgagtcata
    14701 acagcagacg tattgatgtg gatatcaacc ttgataacca ggctgacgat catcgccttc
    14761 gcgtcctgat ccctacacct tttaacaccg acagtgttct ggcagatacg cagtttggtt
    14821 cgctaacgcg ccccgtgaac gacagtgcaa tgaacaactg gcagcaagaa ggctggaaag
    14881 aagcgccggt tccggtatgg aatatgctca actatgttgc cttacaggaa gggcgtaacg
    14941 gcatggctgt ctttagcgaa gggctacgtg aatttgaagt catcggtgaa gagaagaaaa
    15001 cctttgccat tacgttgctg cgtggcgtgg gcttactggg caaagaagat ctgcttttaa
    15061 ggcctgggcg gccttcggga attaaaatgc cagtcccgga ctcacaacta cgtggtctgc
    15121 tttcttgtcg cctaagttta ttgagttata ccggtacgcc aaccgccgct ggtgtagctc
    15181 agcaggcgcg agcatggctg actccagtac agtgttacaa caaaatccca tgggatgcga
    15241 tgaagctcaa caaagccgga ttcaacgtgc cggaaagtta tagtttgttg aaaatgcccc
    15301 cagtgggatg cctgataagc gcacttaaga aagctgaaga ccgacaagaa gtgattttac
    15361 ggctgtttaa tccggctgaa tcagcaacct gtgatgcgac tgttgctttc agtcgcgagg
    15421 tgatttcttg ctcagaaacg atgatggatg aacacattac caccgaggaa aatcaaggtt
    15481 caaatctatc ggggcctttt ttacccggcc agtcacggac gttcagttac cggcttgcct
    15541 gaatagcaat caaaccgaag ccacatatgc gcggccagat tgttgacaaa gggcgctttg
    15601 ttcatgccgg atacggcatg aacgctttat tcggtctaca aaagcaggca aattcaatat
    15661 attgcagaga tgatgtagac actggcaagc gtagcgcatc aggtaatttt gc
//
LOCUS       DAZKHF020000094        15601 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2201S31241
            SAMN43267800-rid25106413.denovo.095, whole genome shotgun sequence.
ACCESSION   DAZKHF020000094 DAZKHF020000000
VERSION     DAZKHF020000094.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267800
            Sequence Read Archive: SRR30307263
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 15601)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15601)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 15601)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350581.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350581.2
            Genome Coverage       :: 151x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:21:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,652
            CDSs (total)                      :: 5,557
            Genes (coding)                    :: 5,320
            CDSs (with protein)               :: 5,320
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 237
            CDSs (without protein)            :: 237
            Pseudo Genes (ambiguous residues) :: 0 of 237
            Pseudo Genes (frameshifted)       :: 102 of 237
            Pseudo Genes (incomplete)         :: 130 of 237
            Pseudo Genes (internal stop)      :: 52 of 237
            Pseudo Genes (multiple problems)  :: 41 of 237
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15601
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267800-rid25106413.denovo.095"
                     /serotype="O 26:H11"
                     /isolate="2201S31241"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-01"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            complement(473..1564)
                     /gene="ychF"
                     /locus_tag="ACHOSC_RS12150"
                     /old_locus_tag="pgaptmp_002430"
     CDS             complement(473..1564)
                     /gene="ychF"
                     /locus_tag="ACHOSC_RS12150"
                     /old_locus_tag="pgaptmp_002430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001538644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="redox-regulated ATPase YchF"
                     /protein_id="HGU4548357.1"
                     /translation="MGFKCGIVGLPNVGKSTLFNALTKAGIEAANFPFCTIEPNTGVV
                     PMPDPRLDQLAEIVKPQRTLPTTMEFVDIAGLVKGASKGEGLGNQFLTNIRETEAIGH
                     VVRCFENDNIIHVSGKVNPADDIEVINTELALADLDTCERAIHRVQKKAKGGDKDAKA
                     ELAVLEKCLPQLENAGMLRALDLSAEEKAAIRYLSFLTLKPTMYIANVNEDGFENNPY
                     LDQVREIAAKEGSVVVPVCAAVEADIAELDDEERDEFMQELGLEEPGLNRVIRAGYKL
                     LNLQTYFTAGVKEVRAWTIPVGATAPQAAGKIHTDFEKGFIRAQTISFEDFITYKGEQ
                     GAKEAGKMRAEGKDYIVKDGDVMNFLFNV"
     gene            complement(1681..2265)
                     /gene="pth"
                     /locus_tag="ACHOSC_RS12155"
                     /old_locus_tag="pgaptmp_002431"
     CDS             complement(1681..2265)
                     /gene="pth"
                     /locus_tag="ACHOSC_RS12155"
                     /old_locus_tag="pgaptmp_002431"
                     /EC_number="3.1.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002910438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004045 - aminoacyl-tRNA hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA hydrolase"
                     /protein_id="HGU4548358.1"
                     /translation="MTIKLIVGLANPGAEYAATRHNAGAWFVDLLAERLRAPLREEAK
                     FFGYTSRVTLGGEDVRLLVPTTFMNLSGKAVAAMASFFRINPDEILVAHDELDLPPGV
                     AKFKLGGGHGGHNGLKDIISKLGNNPNFHRLRIGIGHPGDKNKVVGFVLGKPPVSEQK
                     LIDEAIDEAARCTEMWFTDGLTKATNRLHAFKAQ"
     gene            2543..2821
                     /gene="ychH"
                     /locus_tag="ACHOSC_RS12160"
                     /old_locus_tag="pgaptmp_002432"
     CDS             2543..2821
                     /gene="ychH"
                     /locus_tag="ACHOSC_RS12160"
                     /old_locus_tag="pgaptmp_002432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stress-induced protein YchH"
                     /protein_id="HGU4548359.1"
                     /translation="MKRKNASLLGNVLMGLGLVVMVVGVGYSILNQLPQFNMPQYFAH
                     GAVLSIFVGAILWLAGARVGGHEQVCDRYWWVRHYDKRCRRSDNRRHS"
     gene            complement(2876..4555)
                     /gene="dauA"
                     /locus_tag="ACHOSC_RS12165"
                     /old_locus_tag="pgaptmp_002433"
     CDS             complement(2876..4555)
                     /gene="dauA"
                     /locus_tag="ACHOSC_RS12165"
                     /old_locus_tag="pgaptmp_002433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415724.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C4-dicarboxylic acid transporter DauA"
                     /protein_id="HGU4548360.1"
                     /translation="MNKIFSSHVMPFRALIDACWKEKYTAARFTRDLIAGITVGIIAI
                     PLAMALAIGSGVAPQYGLYTAAVAGIVIALTGGSRFSVSGPTAAFVVILYPVSQQFGL
                     AGLLVATLLSGIFLILMGLARFGRLIEYIPVSVTLGFTSGIGITIGTMQIKDFLGLQM
                     AHVPEHYLQKVGALFMALPTINVGDAAIGIVTLGILVFWPRLGIRLPGHLPALLAGCA
                     VMGIVNLLGGHVATIGSQFHYVLADGSQGNGIPQLLPQLVLPWDLPNSEFTLTWDSIR
                     TLLPAAFSMAMLGAIESLLCAVVLDGMTGTKHKANSELVGQGLGNIIAPFFGGITATA
                     AIARSAANVRAGATSPISAVIHSILVILALLVLAPLLSWLPLSAMAALLLMVAWNMSE
                     AHKVVDLLRHAPKDDIIVMLLCMSLTVLFDMVIAISVGIVLASLLFMRRIARMTRLAP
                     VVVDVPDDVLVLRVIGPLFFAAAEGLFTDLESRLEGKRIVILKWDAVPVLDAGGLDAF
                     QRFVKRLPEGCELRVCNVEFQPLRTMARAGIQPIPGHLAFFPNRRAAMADL"
     gene            complement(4680..5627)
                     /gene="prs"
                     /locus_tag="ACHOSC_RS12170"
                     /old_locus_tag="pgaptmp_002434"
     CDS             complement(4680..5627)
                     /gene="prs"
                     /locus_tag="ACHOSC_RS12170"
                     /old_locus_tag="pgaptmp_002434"
                     /EC_number="2.7.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002910407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate diphosphokinase"
                     /protein_id="HGU4548361.1"
                     /translation="MPDMKLFAGNATPELAQRIANRLYTSLGDAAVGRFSDGEVSVQI
                     NENVRGGDIFIIQSTCAPTNDNLMELVVMVDALRRASAGRITAVIPYFGYARQDRRVR
                     SARVPITAKVVADFLSSVGVDRVLTVDLHAEQIQGFFDVPVDNVFGSPILLEDMLQLN
                     LDNPIVVSPDIGGVVRARAIAKLLNDTDMAIIDKRRPRANVSQVMHIIGDVAGRDCVL
                     VDDMIDTGGTLCKAAEALKERGAKRVFAYATHPIFSGNAANNLRNSVIDEVVVCDTIP
                     LSDEIKSLPNVRTLTLSGMLAEAIRRISNEESISAMFEH"
     gene            complement(5778..6629)
                     /gene="ispE"
                     /locus_tag="ACHOSC_RS12175"
                     /old_locus_tag="pgaptmp_002435"
     CDS             complement(5778..6629)
                     /gene="ispE"
                     /locus_tag="ACHOSC_RS12175"
                     /old_locus_tag="pgaptmp_002435"
                     /EC_number="2.7.1.148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050515 - 4-(cytidine
                     5'-diphospho)-2-C-methyl-D-erythritol kinase activity
                     [Evidence IEA];
                     GO_process: GO:0016114 - terpenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-(cytidine
                     5'-diphospho)-2-C-methyl-D-erythritol kinase"
                     /protein_id="HGU4548362.1"
                     /translation="MRTQWPSPAKLNLFLYITGQRADGYHTLQTLFQFLDYGDTISIE
                     LRDDGDIRLLTPVEGVEHEDNLIVRAARLLMKTAADSGRLPTGSGANISIDKRLPMGG
                     GLGGGSSNAATVLVALNHLWQCGLSMDELAEMGLTLGADVPVFVRGHASFAEGVGEIL
                     TPVDPPEKWYLVAHPGVSIPTPVIFKDPELPRNTPKRSIETLLKCEFSNDCEVIARKR
                     FREVDAVLSWLLEYAPSRLTGTGACVFAEFDTESEARQVLEQAPEWLNGFVAKGVNLS
                     PLHRAML"
     gene            complement(6629..7252)
                     /gene="lolB"
                     /locus_tag="ACHOSC_RS12180"
                     /old_locus_tag="pgaptmp_002436"
     CDS             complement(6629..7252)
                     /gene="lolB"
                     /locus_tag="ACHOSC_RS12180"
                     /old_locus_tag="pgaptmp_002436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_process: GO:0015031 - protein transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein insertase outer membrane protein
                     LolB"
                     /protein_id="HGU4548363.1"
                     /translation="MPLPDFRLIRLLPLAALVLTACSVTTPKGPGKSPDSPQWRQHQQ
                     DVRNLNQYQTRGAFAYISDQQKVYARFFWQQTGQDRYRLLLTNPLGSTELELNAQPGN
                     VQLVDNKGQRYTADDAEEMIGKLTGMPIPLNSLRQWILGLPGDATDYKLDDQYRLSEI
                     TYSQNGKNWKVVYGGYDTKTQPAMPANMELTDGGQRIKLKMDNWIVK"
     gene            7466..8722
                     /gene="hemA"
                     /locus_tag="ACHOSC_RS12185"
                     /old_locus_tag="pgaptmp_002437"
     CDS             7466..8722
                     /gene="hemA"
                     /locus_tag="ACHOSC_RS12185"
                     /old_locus_tag="pgaptmp_002437"
                     /EC_number="1.2.1.70"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_041689438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008883 - glutamyl-tRNA reductase activity
                     [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamyl-tRNA reductase"
                     /protein_id="HGU4548364.1"
                     /translation="MTLLALGINHKTAPVSLRERVSFSPDKLDQALDSLLAQPMVQGG
                     VVLSTCNRTELYLSVEEQDNLQEALIRWLCDYHNLNEEDLRKSLYWHQDNDAVSHLMR
                     VASGLDSLVLGEPQILGQVKKAFADSQKGHMKASELERMFQKSFSVAKRVRTETDIGA
                     SAVSVAFAACTLARQIFESLSTVTVLLVGAGETIELVARHLREHKVQKMIIANRTRER
                     AQILADEVGAEVIALSEIDERLREADIIISSTASPLPIIGKGMVERALKSRRNQPMLL
                     VDIAVPRDVEPEVGKLANAYLYSVDDLQSIISHNLAQRKAAAVEAETIVAQETSEFMA
                     WLRAQSASETIREYRSQAEHVRDELTAKALAALEQGGDAQAIMQDLAWKLTNRLIHAP
                     TKSLQQAARDGDNERLNILRDSLGLE"
     gene            8764..9846
                     /gene="prfA"
                     /locus_tag="ACHOSC_RS12190"
                     /old_locus_tag="pgaptmp_002438"
     CDS             8764..9846
                     /gene="prfA"
                     /locus_tag="ACHOSC_RS12190"
                     /old_locus_tag="pgaptmp_002438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012132158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003747 - translation release factor
                     activity [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 1"
                     /protein_id="HGU4548365.1"
                     /translation="MKPSIVAKLEALHERHEEVQALLGDAQTIADQERFRALSREYAQ
                     LSDVSRCFTDWQQVQEDIETAQMMLDDPEMREMAQDELREAKEKSEQLEQQLQVLLLP
                     KDPDDERNAFLEVRAGTGGDEAALFAGDLFRMYSRYAEARRWRVEIMSASEGEHGGYK
                     EIIAKISGDGVYGRLKFESGGHRVQRVPATESQGRIHTSACTVAVMPELPDAELPDIN
                     PADLRIDTFRSSGAGGQHVNTTDSAIRITHLPTGIVVECQDERSQHKNKAKALSVLGA
                     RIHAAEMAKRQQAEASTRRNLLGSGDRSDRNRTYNFPQGRVTDHRINLTLYRLDEVME
                     GKLDMLIEPIIQEHQADQLAALSEQE"
     gene            9846..10679
                     /gene="prmC"
                     /locus_tag="ACHOSC_RS12195"
                     /old_locus_tag="pgaptmp_002439"
     CDS             9846..10679
                     /gene="prmC"
                     /locus_tag="ACHOSC_RS12195"
                     /old_locus_tag="pgaptmp_002439"
                     /EC_number="2.1.1.297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0018364 - peptidyl-glutamine methylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor N(5)-glutamine
                     methyltransferase"
                     /protein_id="HGU4548366.1"
                     /translation="MEYQHWLREAISQLQASESPRRDAEILLEHVTGRGRTFILAFGE
                     TQLTDEQCQQLDALLTRRRDGEPIAHLTGVREFWSLPLFVSPATLIPRPDTECLVEQA
                     LARLPEQPCRILDLGTGTGAIALALASERPDCEIIAVDRMPDAVSLAQRNAQHLAIKN
                     IHILQSDWFSALAGQQFAMIVSNPPYIDEQDPHLQQGDVRFEPLTALVAADSGMADIV
                     HIIEQSRNALVSGGFLLLEHGWQQGEAVRQAFILAGYHDVETCRDYGDNERVTLGRYY
                     Q"
     gene            10676..11068
                     /gene="sirB2"
                     /locus_tag="ACHOSC_RS12200"
                     /old_locus_tag="pgaptmp_002440"
     CDS             10676..11068
                     /gene="sirB2"
                     /locus_tag="ACHOSC_RS12200"
                     /old_locus_tag="pgaptmp_002440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="invasion regulator SirB2"
                     /protein_id="HGU4548367.1"
                     /translation="MTSFSTLLSVHLISIALSVGLLTLRFWLRYQKHPQAFARWTRIV
                     PPVVDTVLLLSGIALMAKAHILPFSGQAQWLTEKLFGVIIYIVLGFIALDYRRMHSQQ
                     ARIIAFPLALVVLYIIIKLATTKVPLLG"
     gene            11072..11881
                     /gene="sirB1"
                     /locus_tag="ACHOSC_RS12205"
                     /old_locus_tag="pgaptmp_002441"
     CDS             11072..11881
                     /gene="sirB1"
                     /locus_tag="ACHOSC_RS12205"
                     /old_locus_tag="pgaptmp_002441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="invasion regulator SirB1"
                     /protein_id="HGU4548368.1"
                     /translation="MRSLADFEFNKAPLCEGMILACEAIRRDFPSQDVYDELERLVSL
                     AKEEISQLLPLEEQLEKLIALFYGDWGFKASRGVYRLSDALWLDQVLKNRQGSAVSLG
                     AVLLWVANRLDLPLLPVIFPTQLILRIECPDGEIWLINPFNGESLSEHMLDVWLKGNI
                     SPSAELFYEDLDEADNIEVIRKLLDTLKASLMEENQMELALRTSEALLQFNPEDPYEI
                     RDRGLIYVQLDCEHVALNDLSYFVEQCPEDPISEMIRAQINNIAHKHIVLH"
     gene            11917..12771
                     /gene="kdsA"
                     /locus_tag="ACHOSC_RS12210"
                     /old_locus_tag="pgaptmp_002442"
     CDS             11917..12771
                     /gene="kdsA"
                     /locus_tag="ACHOSC_RS12210"
                     /old_locus_tag="pgaptmp_002442"
                     /EC_number="2.5.1.55"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008676 - 3-deoxy-8-phosphooctulonate
                     synthase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-8-phosphooctulonate synthase"
                     /protein_id="HGU4548369.1"
                     /translation="MKQKVVSIGDINVANDLPFVLFGGMNVLESRDLAMRICEHYVTV
                     TQKLGIPYVFKASFDKANRSSIHSYRGPGLEEGMKIFQELKQTFGVKIITDVHEPSQA
                     QPVADVVDVIQLPAFLARQTDLVEAMAKTGAVINVKKPQFVSPGQMGNIVDKFKEGGN
                     EKVILCDRGANFGYDNLVVDMLGFSIMKKVSGNSPVIFDVTHALQCRDPFGAASGGRR
                     AQVAELARAGMAVGLAGLFIEAHPDPEHAKCDGPSALPLAKLEPFLKQMKAIDDLVKG
                     FEELDTSK"
     gene            complement(12920..13027)
                     /locus_tag="ACHOSC_RS12215"
                     /old_locus_tag="pgaptmp_002443"
     CDS             complement(12920..13027)
                     /locus_tag="ACHOSC_RS12215"
                     /old_locus_tag="pgaptmp_002443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin Ldr family
                     protein"
                     /protein_id="HGU4548370.1"
                     /translation="MTLAQFAMIFWHDLAAPILAGIITAAIVGWWRNRK"
     gene            complement(13455..13562)
                     /locus_tag="ACHOSC_RS12220"
                     /old_locus_tag="pgaptmp_002444"
     CDS             complement(13455..13562)
                     /locus_tag="ACHOSC_RS12220"
                     /old_locus_tag="pgaptmp_002444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin Ldr family
                     protein"
                     /protein_id="HGU4548371.1"
                     /translation="MTLAQFAMIFWHDLAASILAGIITAAIVGWWRNRK"
     gene            complement(13991..14098)
                     /locus_tag="ACHOSC_RS12225"
                     /old_locus_tag="pgaptmp_002445"
     CDS             complement(13991..14098)
                     /locus_tag="ACHOSC_RS12225"
                     /old_locus_tag="pgaptmp_002445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_025297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin Ldr family
                     protein"
                     /protein_id="HGU4548372.1"
                     /translation="MTLAQFAMIFWHDLAAPILAGIITAAIVSWWRNRK"
     gene            complement(14503..>15601)
                     /gene="chaA"
                     /locus_tag="ACHOSC_RS12230"
                     /old_locus_tag="pgaptmp_002446"
     CDS             complement(14503..>15601)
                     /gene="chaA"
                     /locus_tag="ACHOSC_RS12230"
                     /old_locus_tag="pgaptmp_002446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="sodium-potassium/proton antiporter ChaA"
                     /protein_id="HGU4548373.1"
                     /translation="SNAQEAVKTRHKETSLIFPVLALVVLFLWGSSQTLPVVIAINLL
                     ALIGILSSAFSVVRHADVLAHRLGEPYGSLILSLSVVILEVSLISALMATGDAAPTLM
                     RDTLYSIIMIVTGGLVGFSLLLGGRKFATQYMNLFGIKQYLIALFPLAIIVLVFPMAL
                     PAANFSTGQALLVALISAAMYGVFLLIQTKTHQSLFVYEHEDDSDDDDPHHGKPSAHS
                     SVWHAIWLIIHLIAVIAVTKMNASPLETLLDSMNAPVAFTGFLVALLILSPEGLGALK
                     AVLNNQVQRAMNLFFGSVLATISLTVPVVTLIAFMTGNELQFALGAPEMVVMVASLVL
                     CHISFSTGRTNVLNGAAHLALFAAYLMTIFA"
BASE COUNT         3909 a         3951 c         3990 g         3751 t
ORIGIN      
        1 atgcgccatt tttgcattac taataagaaa aagcaaaaaa catcgatatt catttttggt
       61 caatgggttt tctggcctat cgttgttatt gtgctactgg ttatcatttt catcttgcca
      121 ggatcgcacg taatcacgtt attcggggac tttatctgcg ggatattacc cttcaggtaa
      181 gtccttttct cgccagcata ttggagcgtg atcaatattg atcagctgtg aagggggtga
      241 tgtgtagtgg accaaaactt acgttatatc actactaatg agatatccgt catctgacac
      301 tgatgtcttt aattattctc gtggttcact gtaggcaata agcacaaaag tgtaggatgt
      361 tacaagaatg attaggactc ggtgaaatga aaaatccacg caattgcgtg gattatatat
      421 acttttgcgc tcttcatgag atttagcgaa acctcatgag acaacaaatt aattagacgt
      481 tgaaaaggaa gttcatcaca tcgccatctt taacgatgta atctttacct tctgcacgca
      541 ttttgcctgc ttctttcgcg ccttgttcac ctttgtaagt gatgaaatct tcaaacgaga
      601 tggtttgtgc acggataaag cctttttcaa aatcagtatg gattttgccc gctgcctgcg
      661 gcgcggttgc tccaaccgga atggtccatg cacgcacttc tttcacccca gcggtgaagt
      721 aagtttgcag gttcagcagc ttataaccgg cacggatcac acggttcagg cccggctctt
      781 ccagcccaag ctcctgcata aactcgtcac gttcttcgtc gtccagttcg gcaatgtctg
      841 cttcaacagc agcacaaacc ggaaccacaa cagaaccttc tttcgccgcg atttcacgca
      901 cctggtcaag atatgggttg ttttcaaaac cgtcttcgtt gacgttggcg atgtacattg
      961 ttggtttcag cgtcaggaag ctcaggtaac gaatagccgc tttctcttca gcgcttaaat
     1021 ccagcgcgcg cagcatacct gcattttcca actggggcag gcatttttcc aggaccgcca
     1081 gctcagcttt cgcgtcttta tcgccacctt tggctttctt ctgtacgcga tgaatcgcac
     1141 gttcgcaggt gtcgaggtct gccagcgcca gttcggtgtt gataacttca atatcgtcag
     1201 ccgggttaac tttgccggaa acgtgaatga tgttgtcatt ttcaaagcag cgaacaacgt
     1261 gaccgatcgc ttcggtttca cggatgttgg tcaggaactg gttacccaga ccttcgcctt
     1321 tcgatgcgcc ttttaccaga ccggcgatat cgacaaattc catggtcgtg ggaagcgtac
     1381 gttgcggttt tacgatttca gccagttgat ccaggcgagg gtcaggcatt ggtacgacgc
     1441 ctgtgttcgg ctcaatggtg cagaatggaa agttggccgc ttcaataccg gctttggtca
     1501 gcgcgttgaa cagggtagat ttcccgacgt tgggcaaacc gacgataccg catttgaatc
     1561 ccatgattta aatcacctta atatcttaat aatcaacctg ttattgctaa cagattgcag
     1621 aaatggaaat aactttgcct attatacacg gcactcggca aaaatgccgc agacaacgac
     1681 ttattgcgct ttaaaggcgt gcaatcggtt cgttgctttg gtcaagccat ctgtaaacca
     1741 catttcagta caacgcgccg cttcgtcgat ggcttcatca attaacttct gttcactaac
     1801 aggcggtttg cctaacacaa aaccgacaac tttattttta tcgcccggat gaccgattcc
     1861 gatgcgtaaa cggtgaaagt tagggttatt acccaattta ctgatgatgt ctttcagtcc
     1921 attgtgacca ccatggccac cgcccaattt aaatttggcg acgccaggag gcagatccag
     1981 ttcgtcgtgg gccaccagaa tttcgtccgg attaatgcgg aaaaaactgg ccatcgccgc
     2041 aacggctttg ccgctgagat tcataaatgt agtcgggact aacaggcgga catcttcgcc
     2101 tccaagagtg actcgcgaag tataaccaaa gaatttagcc tcttcgcgca gcggagcgcg
     2161 caaacgctct gccagtaagt caacgaacca ggcaccagca ttatgtcgcg ttgcggcgta
     2221 ttcagcaccg gggttcgcca ggccgacaat caatttaatc gtcacgtttt tttgtcctga
     2281 gtgtgtacat aactggcgcg tagtttactg gttgcggccc cgcttgacaa aaaactgcgt
     2341 atcaaatgca gataacgtaa taattgcatg agtggactat tagaaagtca aggtgttcag
     2401 gcgtttattt gtaaagtttt gttgaaataa gggttgtaat tgtgatcacg cccgcacata
     2461 acccacaggg tgttgtctat actttacaca taaggaagag gggtattccc tgttacaacc
     2521 cagaaagttc cggaggtgac atatgaaacg caaaaacgct tcgttactcg gtaacgtgct
     2581 catggggttg ggtctggtgg taatggtggt cggcgtgggg tattcaatcc tcaaccagtt
     2641 accacagttt aatatgcccc agtatttcgc acatggtgca gtgctaagta ttttcgtcgg
     2701 tgccattctc tggctggcgg gtgcccgtgt tggcgggcat gaacaggtgt gcgaccgtta
     2761 ctggtgggtt cgccactatg acaaacgttg ccgccgtagc gataatcgcc gtcatagcta
     2821 acaaataatg cagtttgctg accagtcagt ttgcgctgac tggtcaatct cgtacttata
     2881 aatccgccat cgccgcgcga cgattcggga agaacgccag gtgtcccggg atcggttgaa
     2941 tgccagcgcg agccatagtg cgcagtggct ggaattccac gttgcacacg cgcagttcac
     3001 atccctcggg cagacgcttc acaaaacgct ggaacgcatc aagaccacca gcatcaagta
     3061 ccggaaccgc atcccacttc agaatcacaa tccgtttgcc ttcaagacgt gactccaggt
     3121 ccgtgaacaa gccttcagca gcagcaaaaa acagcgggcc aataacgcgc agaaccagga
     3181 catcgtctgg aacatctacg actaccggtg ccaggcgagt catacgtgcg atacgacgca
     3241 taaacagcag cgatgccagc acgatcccca cgctgatggc aataaccata tcaaacagca
     3301 cggtcagcga catgcacagc agcatgacaa tgatgtcatc tttcggcgca tgacgcagca
     3361 agtcgaccac tttgtgcgct tcactcatgt tccacgccac catcaacagc agggcagcca
     3421 tggcggaaag cggcagccag gagagcagcg gtgccagtac cagcagggca agaataacca
     3481 gaatagagtg gatcaccgcc gagatagggg aagttgcccc ggcacggacg ttagcggcag
     3541 aacgcgcgat ggcagctgta gcggtaatgc caccaaagaa cggagcgatg atattcccca
     3601 gtccctgtcc aaccagttcg ctattcgcct tgtgtttcgt cccggtcata ccatccagca
     3661 ccacggcgca gagcagagat tcgattgcgc cgagcattgc cattgagaat gccgcaggca
     3721 gcagtgtgcg aatagaatcc caggttagcg tgaattctga attaggcaga tcccacggca
     3781 gcaccagttg cggcagcagt tgcggaatac cgttaccctg agaaccatcg gccaggacgt
     3841 agtggaattg cgaaccgatg gtagcaacat gtccgccgag caggttaaca atccccatca
     3901 ccgcgcaacc agccagcaag gccggaaggt gaccgggtaa acgaatgccc agacgcggcc
     3961 agaaaacaag aatacctagc gtcacaatgc caatggcagc atcacccaca ttaatggtcg
     4021 gcagcgccat aaataatgcg ccgacttttt gtagataatg ttccgggaca tgggccattt
     4081 gcagaccgag aaaatcttta atctgcatgg taccgatggt gatcccgata cccgaggtga
     4141 aacctaaggt gacggaaacc ggaatatact caatcaggcg accaaagcgt gccagaccca
     4201 tcagaatcaa aaaaatcccc gacagcaagg tcgcaaccag cagtcctgcc agtccaaact
     4261 gttgcgacac gggatagaga attaccacaa atgccgcagt cggaccggaa acgctaaagc
     4321 gtgacccacc cgtcagagca atgacaatcc ccgcaacagc tgcggtatat aaaccgtact
     4381 ggggtgccac accactacca atagccaacg ccatcgccag cgggatagca ataatcccga
     4441 cggttatccc ggcaatcagg tcacgggtaa accgtgcggc agtatatttt tctttccagc
     4501 aagcgtcgat cagagcgcgg aaaggcatca catgtgagga aaatattttg ttcacaataa
     4561 tgtttcatcc gtgagcgcat catctgtcaa ctaaatggca ggtgaaggag gcataggtca
     4621 tacaaatgga tattacagac aaaaaaaccc gccgcagcgg gtctttgagc cgggttcgat
     4681 tagtgttcga acatggcaga gatcgattct tcgttgctga tacgacgaat cgcttcggcc
     4741 agcatacctg acagggtcag agtacgcacg ttcggcagtg atttgatttc atcgctcagc
     4801 ggaatggtat cgcagacaac gacttcatca attacagagt tacgcaggtt attcgccgcg
     4861 ttgccagaga agatcgggtg agtcgcgtac gcaaatacac gtttagcacc acgttctttc
     4921 agagcttcag cagctttaca cagcgtaccg ccagtgtcga tcatatcatc gaccagtacg
     4981 cagtcacgac ctgcaacgtc accgatgata tgcatcacct gggaaacgtt cgcacgcgga
     5041 cgacgtttgt cgatgattgc catatcggta tcgttcagca gcttagcgat agcgcgggca
     5101 cgcacaacgc cgccgatgtc cggagaaacc acaattgggt tatccagatt cagctgcagc
     5161 atgtcttcca gcaggatcgg gctaccaaat acgttatcaa ccggaacgtc gaagaaaccc
     5221 tgaatctgtt cagcgtgcag atccactgtc agcacacggt caacaccgac gctggagagg
     5281 aagtctgcaa ccactttcgc agtgattggt acacgagcgg aacggacgcg acggtcctgg
     5341 cgcgcatagc caaagtaggg gataacagcg gtgatacgac ctgcggaagc acgacgcagg
     5401 gcatcaacca taacgactaa ttccatcagg ttgtcgttag taggggcaca agtggactgg
     5461 atgatgaaaa tatcaccacc gcgtacattt tcgttgattt gtacactgac ttcgccgtcg
     5521 ctaaagcgac ctacagcggc gtcgccgagt gaagtgtaca ggcggttggc aatacgttgt
     5581 gctagttccg gggtggcgtt accagcaaaa agcttcatat caggcacgag aagaacctca
     5641 ggcatgcgtc cattggtgga aagaatctgc cgaaaactgt gcgggccagg catgatcctt
     5701 tccaggcggt gtattaaaga gcgcgatgca acgtctggaa caaggtgacg ttgtcaccga
     5761 aactcagctt gcccggctta aagcatggct ctgtgcaatg gggaaagatt aacgcctttc
     5821 gccacaaagc cattgagcca ttccggggct tgctctagca cctggcgggc ttcagactct
     5881 gtatcaaatt cagcaaagac acaggcccct gtcccagtca ggcgcgacgg ggcgtattct
     5941 aacagccagg aaagcaccgc atcaacctcg cgaaaacgtt ttcttgcgat aacctcgcaa
     6001 tcattgctga attcacattt tagcaacgtt tctattgacc tttttggcgt attgcgcggg
     6061 agttcaggat ctttaaaaat caccggagtc ggaatactta caccagggtg cgccaccaga
     6121 taccacttct ctggcggatc caccggcgtt agtatttcac caacgccttc ggcaaacgag
     6181 gcatgccccc gaacaaagac aggaacatct gcgcccagcg tcagccccat ttccgccagc
     6241 tcatccatgc ttagcccgca ttgccagaga tgatttaatg ccaccaggac cgtcgcggca
     6301 ttggatgaac caccgccgag accgccgccc atcggcaaac gcttgtcaat gctgatattc
     6361 gcaccgcttc ccgtcggaag acgcccgctg tctgccgcag ttttcatcaa caatcgcgct
     6421 gcgcgaacga tcaggttatc ttcatgttcc acgccttcaa cgggcgttaa cagacgaata
     6481 tccccatcgt cacgaagctc aatgctgatg gtgtcgccgt aatcaagaaa ctgaaacagc
     6541 gtttgcagcg tgtggtaacc atccgcacgc tgaccggtaa tgtataaaaa cagattaagt
     6601 tttgccggag agggccactg tgtccgcatt atttcactat ccagttatcc atttttaact
     6661 tgatgcgttg accaccgtcg gtgagttcca tattggctgg catcgcaggt tgcgttttgg
     6721 tgtcataacc accataaaca accttccagt ttttgccatt ctggctgtag gtaatttcgc
     6781 tcaggcggta ctggtcgtcc agtttgtagt cggttgcatc acccggtaaa cctaaaatcc
     6841 actggcgcaa gctgttgagc ggaattggca ttccggtcaa tttgccaatc atctcttcgg
     6901 cgtcatcggc ggtataacgc tgacctttat tgtcgactaa ctgcacgtta cccggttgag
     6961 cattcagctc cagttccgtg ctgcccaatg ggttagtgag cagcagacgg tagcgatcct
     7021 ggccggtttg ctgccagaaa aagcgggcgt acactttttg ttggtcagaa atataagcga
     7081 acgcgccgcg agtctgatac tgattaagat tgcgcacgtc ttgctgatgc tgacgccatt
     7141 gtggcgaatc cgggcttttg ccaggacctt tgggcgtggt aacggaacag gcagtgagca
     7201 caagagcagc cagcggtagc aggcggataa gacgaaaatc gggcaggggc atagtgatga
     7261 caagtccttg agatacgttg cagttataac ccttaatgct agcgttaccg tccgctatcg
     7321 tctatgttca agttgtctta attgccagaa tctaacggct ttcggcaatt actccaaaag
     7381 ggggcgctct cttttattga tcttacgtat cctgtatgat gcaagcagac taaccctatc
     7441 aacgttggta ttatttcccg cagacatgac ccttttagca ctcggtatca accataaaac
     7501 ggcacctgta tcgctgcgag aacgtgtatc gttttcgccg gataagctcg atcaggcgct
     7561 tgacagcctg cttgcgcagc cgatggtgca gggcggcgtg gtgctgtcga cgtgcaaccg
     7621 cacggaactt tatcttagcg ttgaagagca ggataacctg caagaggcgt taatccgctg
     7681 gctttgcgat tatcacaatc ttaatgaaga agatctgcgt aaaagcctct actggcatca
     7741 ggataacgac gcggttagcc atttaatgcg tgttgccagc ggcctagatt cactggttct
     7801 tggggagccg cagatcctcg gtcaggttaa aaaagcgttt gccgattcgc aaaaaggtca
     7861 tatgaaggcc agcgaactgg aacgcatgtt ccagaaatct ttctctgtag cgaaacgcgt
     7921 tcgcactgaa acagatatcg gtgccagtgc tgtgtctgtc gcttttgcgg cttgtacgct
     7981 ggcgcggcag atctttgaat cgctctctac ggtcacagtg ttgctggtag gcgcgggcga
     8041 aaccatcgag ctggtagcgc gtcatctgcg cgaacataaa gtacagaaga tgattatcgc
     8101 caaccgcact cgcgaacgtg cccaaattct ggcagatgaa gttggcgcgg aagtgattgc
     8161 cctgagtgag atcgacgaac gtctgcgcga agccgatatc atcatcagtt ccaccgccag
     8221 cccgttaccg attatcggga aaggcatggt ggagcgcgca ttaaaaagcc gtcgcaacca
     8281 accaatgctg ttggtggata ttgccgttcc gcgcgatgtt gagccggaag ttggcaaact
     8341 ggcgaatgct tatctttata gtgtggacga tctgcaaagc atcatttcgc acaacctggc
     8401 gcagcgtaaa gccgcagcgg ttgaggcgga aactattgtc gctcaggaaa ccagcgaatt
     8461 tatggcgtgg ctgcgagcac aaagcgccag cgaaaccatt cgcgagtatc gcagccaggc
     8521 agagcacgtt cgcgatgagt taaccgccaa agcgttagcg gcccttgagc agggcggcga
     8581 cgcgcaagcc attatgcagg atctggcatg gaaactgact aaccgcttga tccatgcgcc
     8641 aacgaaatca cttcaacagg ccgcccgtga cggggataac gaacgcctga atattctgcg
     8701 cgacagcctc gggctggagt agcagtacat cattttcttt ttttacaggg tgcatttacg
     8761 cctatgaagc cttctatcgt tgccaaactg gaagccctgc atgaacgcca tgaagaagtt
     8821 caggcgttgc tgggtgacgc gcaaactatc gccgaccagg aacgttttcg cgcattatca
     8881 cgcgaatatg cgcagttaag tgatgtttcg cgctgtttta ccgactggca acaggttcag
     8941 gaagatatcg aaaccgcaca gatgatgctc gatgatcctg aaatgcgtga gatggcgcag
     9001 gatgaactgc gcgaagctaa agaaaaaagc gagcaactgg aacagcaatt acaggttctg
     9061 ttactgccaa aagatcctga tgacgaacgt aacgccttcc tcgaagtccg agccggaacc
     9121 ggcggcgacg aagcggcgct gttcgcaggc gatctgttcc gtatgtacag ccgttacgcc
     9181 gaagcccgcc gctggcgggt agaaatcatg agcgccagcg agggtgaaca tggtggttat
     9241 aaagagatca tcgccaaaat tagcggtgat ggtgtgtatg gtcgtctgaa atttgaatct
     9301 ggcggtcatc gcgtgcagcg tgttcctgct acggaatcgc agggtcgtat tcatacttct
     9361 gcttgtaccg ttgcggtaat gccagaactg cctgacgcag aactgccgga catcaaccca
     9421 gcagatttac gcattgatac tttccgctcg tcaggggcgg gtggtcagca cgttaacacc
     9481 accgattcgg caattcgtat tactcacttg ccgaccggga ttgttgttga atgtcaggat
     9541 gaacgttcac aacataaaaa caaagcgaag gcgctttcag tgctcggtgc tcgcatccac
     9601 gctgctgaaa tggcaaaacg ccaacaggcc gaagcgtcta cccgtcgtaa cctgctgggg
     9661 agtggcgatc gcagcgaccg taaccgtact tacaatttcc cgcaggggcg cgttaccgat
     9721 caccgcatca acctgacgct ctaccgcctg gatgaagtga tggaaggtaa gctggatatg
     9781 ctgattgaac cgattatcca ggaacatcag gccgaccaac tggcggcgtt gtccgagcag
     9841 gaataatgga atatcaacac tggttacgtg aagcaataag ccaacttcag gcgagcgaaa
     9901 gcccgcggcg tgatgctgaa atcctgctgg agcatgttac cggcagaggg cgtactttta
     9961 ttctcgcctt tggtgaaacg cagctgactg acgaacaatg tcagcaactt gatgcgctac
    10021 tgacacgtcg tcgcgatggt gaacccattg ctcatttaac cggggtgcga gaattctggt
    10081 cgttgccgtt atttgtttcg ccagcgacct taattccgcg cccggatacg gagtgtctgg
    10141 tggagcaggc actggcgcgg ttgcctgaac aaccttgccg tattcttgat ctcgggacgg
    10201 gtaccggggc gattgcgctt gcgctggcta gcgagcgccc ggactgcgaa attatcgctg
    10261 tagatcgtat gcctgatgct gtctccctgg cacaacgtaa tgcccagcat ctggcgatca
    10321 aaaatatcca cattctgcaa agcgactggt ttagcgcgct agccgggcag cagtttgcga
    10381 tgattgtcag caatccgccg tatattgacg agcaggaccc acatcttcaa caaggcgatg
    10441 tccgctttga gccgctcact gcgctggttg cggcagacag tggaatggca gacatcgtgc
    10501 atatcatcga acagtcgcgt aacgcgctgg tatccggcgg ctttctgctt ctggaacatg
    10561 gctggcagca gggcgaagcg gtgcgacaag catttatcct cgcggggtat catgacgtcg
    10621 aaacctgccg tgactatggt gataacgagc gcgtaacgct cggccgctat tatcaatgac
    10681 aagtttttct acactgctta gtgttcatct tattagtatc gcgctttctg ttgggctatt
    10741 aaccttacgt ttctggctac gttatcagaa gcatccacag gcatttgctc gctggacgcg
    10801 cattgtgccg ccggttgtcg atacggtgtt attgttaagc ggcattgcgt tgatggctaa
    10861 agcgcacatc ctgccatttt ccgggcaggc acagtggctg actgaaaagc tgtttggagt
    10921 tatcatttat atcgttttgg gttttattgc actcgattat cgtcgtatgc acagtcagca
    10981 ggcgcgcatt attgccttcc cgctggcgtt ggtggtgctg tacatcatca ttaaactcgc
    11041 caccacaaaa gtaccgttac tggggtaagt catgagatcg ttagctgatt tcgaatttaa
    11101 taaagcgcca ttgtgcgaag gcatgatcct ggcttgcgaa gcaatccgcc gcgattttcc
    11161 ctcgcaagat gtttacgacg aactggagcg tctcgttagt ctggcgaagg aagaaatcag
    11221 ccagcttctg cctttagaag agcagctgga aaaactgatc gcgctgtttt acggcgactg
    11281 gggatttaaa gcctcacgcg gcgtttatcg tctttccgat gcattatggc tggaccaggt
    11341 gttaaagaat cgacagggca gtgcggtatc attaggtgcg gttttattat gggtcgcgaa
    11401 tcgtctcgat ttgccgctgc tgccggtgat tttccctacg cagctgatat tgcgcattga
    11461 atgtccggat ggcgaaattt ggctgattaa tccttttaac ggtgaatcgt taagcgaaca
    11521 tatgctggac gtatggttaa agggaaatat cagcccgtcg gcggaactgt tttatgaaga
    11581 ccttgatgaa gctgataaca ttgaggtaat ccgcaaattg ctggatacac tcaaagcctc
    11641 gttgatggaa gaaaatcaga tggagctggc gttacgcacc agcgaagctt tattacaatt
    11701 caaccctgaa gatccctatg aaattcgcga tcgcgggttg atttatgtgc aactggattg
    11761 cgaacacgtt gcgttgaacg atttaagtta tttcgttgaa cagtgtccgg aagacccgat
    11821 cagcgaaatg atccgtgcgc aaataaataa catcgcgcat aaacatattg tgctgcatta
    11881 attaatcgac attttactca agattaaggc gatcctatga aacaaaaagt ggttagcatt
    11941 ggcgacatca acgtagcaaa tgacctgccg ttcgtactgt ttggcggtat gaacgtgctg
    12001 gaatctcgcg atctggcgat gcgcatttgc gagcactacg taactgtgac ccagaaactg
    12061 ggtatccctt acgtgttcaa agcctctttt gacaaagcca accgctcctc catccactct
    12121 tatcgtggac cgggcctgga agaagggatg aaaatcttcc aggagttgaa gcagactttt
    12181 ggcgtgaaaa ttatcaccga cgttcacgaa ccaagtcagg cacagcccgt tgctgatgtc
    12241 gtggatgtga ttcagttgcc ggcgtttctt gctcgccaga ctgacctggt tgaagccatg
    12301 gcgaaaaccg gtgcggtaat taacgtcaag aaaccacagt ttgtcagccc gggacagatg
    12361 ggtaatatcg ttgataaatt caaagaaggc ggcaacgaaa aagtgattct ttgcgatcgt
    12421 ggtgctaact tcggctatga caacctggtt gtcgatatgc tgggcttcag cattatgaag
    12481 aaagtgtctg gtaactcgcc ggtgattttc gacgtgaccc acgcactgca atgccgcgat
    12541 ccgtttggcg cagcttccgg tggtcgtcgt gctcaggtgg ctgagctggc acgagccggt
    12601 atggcggtag gtctggcggg gctgtttatt gaagcgcatc cggatccgga acatgcgaaa
    12661 tgtgatggtc catccgcgct gccgctggct aaactggaac cgttcctcaa gcagatgaaa
    12721 gcgattgatg atctggtgaa aggtttcgaa gaactggata ccagcaagta atcttttttg
    12781 cttgaaaaat aaagtcttag cgttctgcgt taagactttt ttcatgggtg ccagatacaa
    12841 aaaaggccgc aggctgttac ccctgcggcc ggtttcgggc gcatattgcc atcacggcag
    12901 cctgacgccc gttttcacct tacttccggt tacgccacca gccgacaatc gctgcggtaa
    12961 taattcccgc caggatcggt gctgccaggt cgtgccagaa aatcatggca aactgcgcga
    13021 gcgtcatata gccgccttgt tgtaatgaca acattttgcg gctattcttg aattgttctg
    13081 gttcaagatt agcccccgtt ctgttgtcag gttaaacctc tcaacgtgcg ggggttttct
    13141 ctttccagca accaatgcca ccagggataa agcccccgca acattgcgcc tcaccggata
    13201 acgccggctt gatgtggata ctacgtctca attcatcttc acttcatccc tgaaatgttt
    13261 gcaatgaaga gtgcattccg gtttttcaac agctgttaca gtcgtttcat gagggctctg
    13321 gatgatgctt ccagctcggg ttgccaatgt ttactggcgg aagagataaa gacaaaaaag
    13381 gccgcaggct gttacccctg cggccggttt cgggcgcata ttgccatcac ggcagcctga
    13441 cgcccgtttt cgccttactt ccggttacgc caccagccga caatcgctgc ggtaataatt
    13501 cccgccagga tcgatgctgc caggtcgtgc cagaaaatca tggcaaactg cgcgagcgtc
    13561 atatagccgc cttgttgtaa tgacaacatt ttgcggctat tcttgaattg ttgatggttc
    13621 aagattagcc cccgttctgt tgtcaggttt tacctctcaa cgtgcggggg ttttctcttt
    13681 ccagcaacca atgccaccag ggataaagcc cccgcaacat tgcgcctcac cggataacgc
    13741 cggcttggtg tggatactac gactcaattc atcttcactt catccctgaa atgtttgcaa
    13801 tgaagagtgc attccggttt ttcaacagct gttacagtcg tttcatgagt gctctggatg
    13861 atgcttccag ctcgggttgc caatatttac ttgtggaaga gataaagaca aaaatggccg
    13921 caggctgtta cccctgcggc cggtttcggg cgcatattgc catcacggca gcctgacgcc
    13981 cgttttcacc ttacttccgg ttacgccacc agctgacaat cgctgcggta ataattcccg
    14041 ccaggatcgg tgctgccagg tcgtgccaga aaatcatggc aaactgcgcg agcgtcatat
    14101 agccgccttg ttgtaatgac aacattttgc ggctattctt gaagtgtctg gtttcaagat
    14161 tagcccccgt tttgttgtca ggttttacct ctcaacgtgc gggggttttc tctttccagc
    14221 aaccaatgcc accagggata aagcccccgc aacattgcgc ctcaccggat aacgccggct
    14281 tggtgtggat actacgtcgc aattcatctt cacttcatcc ctgaaatgtt tgcaataaag
    14341 agtacattcc ggcttttcaa cagctgttgc agtggtttcg tgcaggttgt ggtacaggct
    14401 cgcaattctg attaacgagc tgtctaggta gtatgaagaa atacaataag caggaattta
    14461 tcttatctcc cccgatttcc gtcatcggag gagatataac tgtcaggcaa atatcgtcat
    14521 caaataggcg gcaaacagtg ccagatgcgc tgcgccattg agcacgttag tacgtccggt
    14581 ggagaaggag atatggcaca gcactaaaga ggccaccatc accaccattt ctggcgcacc
    14641 aagtgcaaac tgtaattcgt tacccgtcat aaaggcaatt agcgtgacga caggtacggt
    14701 aagcgaaatg gttgctaaca ctgaaccaaa gaacagattc atcgcgcgct ggacctggtt
    14761 gttcaacact gcttttaacg cacctaaacc ttccggcgac agaatcaaca gagccaccag
    14821 gaagccagta aaggcgacag gggcattcat gctgtcgagc aatgtctcca gcgggctggc
    14881 attcattttg gtgactgcaa taacggcaat cagatggata atcaaccaga tagcatgcca
    14941 cacgctgcta tgggcagacg gtttaccgtg atgcgggtca tcgtcatcac tgtcatcttc
    15001 gtgctcgtag acaaacaaac tttgatgcgt tttggtctgg atcagcaaaa atacgccata
    15061 cattgccgca gaaattaatg ctaccagtaa cgcctgaccg gttgaaaaat tcgccgcagg
    15121 cagagccatt ggaaatacca gtacgattat cgccaggggg aacagcgcaa ttaaatactg
    15181 cttgatacca aacagattca tatattgggt ggcaaactta cgaccgccca acaataatga
    15241 aaagccaacc agcccaccgg taacaatcat aatgattgaa tagagcgtat cacgcattag
    15301 cgttggcgcg gcgtcgccgg ttgccattaa agctgaaatc aaactgactt caagaataac
    15361 cactgaaagg ctaagaataa gcgaaccgta aggttctccc aggcgatggg ctaatacgtc
    15421 cgcatgacgg acaacactaa aggcgctact taaaatacca ataagcgcaa gaagattgat
    15481 ggcaatgacc actggtagtg tctggctgct tccccacagg aacagcacta ccagcgccag
    15541 aaccgggaaa ataagcgaag tctccttgtg gcgggttttt accgcctctt gagcatttga
    15601 c
//
LOCUS       DAZKHF020000095        15324 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2201S31241
            SAMN43267800-rid25106413.denovo.096, whole genome shotgun sequence.
ACCESSION   DAZKHF020000095 DAZKHF020000000
VERSION     DAZKHF020000095.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267800
            Sequence Read Archive: SRR30307263
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 15324)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15324)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 15324)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350581.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350581.2
            Genome Coverage       :: 151x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:21:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,652
            CDSs (total)                      :: 5,557
            Genes (coding)                    :: 5,320
            CDSs (with protein)               :: 5,320
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 237
            CDSs (without protein)            :: 237
            Pseudo Genes (ambiguous residues) :: 0 of 237
            Pseudo Genes (frameshifted)       :: 102 of 237
            Pseudo Genes (incomplete)         :: 130 of 237
            Pseudo Genes (internal stop)      :: 52 of 237
            Pseudo Genes (multiple problems)  :: 41 of 237
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15324
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267800-rid25106413.denovo.096"
                     /serotype="O 26:H11"
                     /isolate="2201S31241"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-01"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            complement(158..964)
                     /gene="trpA"
                     /locus_tag="ACHOSC_RS12235"
                     /old_locus_tag="pgaptmp_002447"
     CDS             complement(158..964)
                     /gene="trpA"
                     /locus_tag="ACHOSC_RS12235"
                     /old_locus_tag="pgaptmp_002447"
                     /EC_number="4.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004834 - tryptophan synthase activity
                     [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan synthase subunit alpha"
                     /protein_id="HGU4548374.1"
                     /translation="MERYESLFAQLKERKEGAFVPFVTLGDPGIEQSLKIIDTLIEAG
                     ADALELGIPFSDPLADGPTIQNATLRAFAAGVTPAQCFEMLALIRQKHPTIPIGLLMY
                     ANLVFNKGIDEFYAQCEKVGVDSVLVADVPVEESAPFRQAALRHNVAPIFICPPNADD
                     DLLRQIASYGRGYTYLLSRAGVTGAENRAALPLNHLVAKLKEYNAAPPLQGFGISAPD
                     QVKAAIDAGAAGAISGSAIVKIIEQHINEPEKMLAALKVFVQPMKAATRS"
     gene            complement(964..2157)
                     /gene="trpB"
                     /locus_tag="ACHOSC_RS12240"
                     /old_locus_tag="pgaptmp_002448"
     CDS             complement(964..2157)
                     /gene="trpB"
                     /locus_tag="ACHOSC_RS12240"
                     /old_locus_tag="pgaptmp_002448"
                     /EC_number="4.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004834 - tryptophan synthase activity
                     [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan synthase subunit beta"
                     /protein_id="HGU4548375.1"
                     /translation="MTTLLNPYFGEFGGMYVPQILMPALRQLEEAFVSAQKDPEFQAQ
                     FNDLLKNYAGRPTALTKCQNITAGTNTTLYLKREDLLHGGAHKTNQVLGQALLAKRMG
                     KTEIIAETGAGQHGVASALASALLGLKCRIYMGAKDVERQSPNVFRMRLMGAEVIPVH
                     SGSATLKDACNEALRDWSGSYETAHYMLGTAAGPHPYPTIVREFQRMIGEETKAQILE
                     REGRLPDAVIACVGGGSNAIGMFADFINETNVGLIGVEPGGHGIETGEHGAPLKHGRV
                     GIYFGMKAPMMQTEDGQIEESYSISAGLDFPSVGPQHAYLNSTGRADYVSITDDEALE
                     AFKTLCLHEGIIPALESSHALAHALKMMRENPDKEQLLVVNLSGRGDKDIFTVHDILK
                     ARGEI"
     gene            complement(2169..3530)
                     /gene="trpCF"
                     /locus_tag="ACHOSC_RS12245"
                     /old_locus_tag="pgaptmp_002449"
     CDS             complement(2169..3530)
                     /gene="trpCF"
                     /locus_tag="ACHOSC_RS12245"
                     /old_locus_tag="pgaptmp_002449"
                     /EC_number="4.1.1.48"
                     /EC_number="5.3.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004425 - indole-3-glycerol-phosphate
                     synthase activity [Evidence IEA];
                     GO_function: GO:0004640 - phosphoribosylanthranilate
                     isomerase activity [Evidence IEA];
                     GO_process: GO:0006568 - tryptophan metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional indole-3-glycerol-phosphate
                     synthase TrpC/phosphoribosylanthranilate isomerase TrpF"
                     /protein_id="HGU4548376.1"
                     /translation="MMQTVLAKIVADKAIWVETRKQQQPLASFQNEVQPSTRHFYDAL
                     QGARTAFILECKKSSPSKGVIRDDFDPARIAAIYKHYASAISVLTDEKYFQGSFDFLP
                     IVSQIAPQPILCKDFIIDPYQIYLARYYQADACLLMLSVLDDEQYRQLAAVAHSLEMG
                     VLTEVSNEEELERAIALGAKVVGINNRDLRDLSIDLNRTRELAPKLGHNVTVISESGI
                     NTYAQVRELSHFANGFLIGSALMAHDDLHAAVRRVLLGENKVCGLTRGQDAKAAYDAG
                     AIYGGLIFVATSPRCVNVEQAQEVMAAAPLQYVGVFRNHDIADVVDKAKVLSLAAVQL
                     HGNEEQLYIDTLREALPAHVAIWKALSVGETLPAREFQHVDKYVLDNGQGGSGQRFDW
                     SLLNGQSLGNVLLAGGLGADNCVEAAQTGCAGLDFNSAVESQPGIKDARLLASVFQTL
                     RAY"
     gene            complement(3531..5126)
                     /gene="trpD"
                     /locus_tag="ACHOSC_RS12250"
                     /old_locus_tag="pgaptmp_002450"
     CDS             complement(3531..5126)
                     /gene="trpD"
                     /locus_tag="ACHOSC_RS12250"
                     /old_locus_tag="pgaptmp_002450"
                     /EC_number="2.4.2.18"
                     /EC_number="4.1.3.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008784940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004048 - anthranilate
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional anthranilate synthase glutamate
                     amidotransferase component TrpG/anthranilate
                     phosphoribosyltransferase TrpD"
                     /protein_id="HGU4548377.1"
                     /translation="MADILLLDNIDSFTYNLADQLRSNGHNVVIYRNHIPAQTLIERL
                     ATMSNPVLMLSPGPGVPSEAGCMPELLTRLRGKLPIIGICLGHQAIVEAYGGYVGQAG
                     EILHGKASSIEHDGQAMFAGLTNPLPVARYHSLVGSNIPAGLTINAHFNGMVMAVRHD
                     ADRVCGFQFHPESILTTQGARLLEQTLAWAQQKLEPANTLQPILEKLYQAQTLSQQES
                     HQLFSAVVRGELKPEQLAAALVSMKIRGEHPNEIAGAATALLENAAPFPRPDYLFADI
                     VGTGGDGSNSINISTASAFVAAACGLKVAKHGNRSVSSKSGSSDLLAAFGINLDMNAD
                     KSRQALDELGVCFLFAPKYHTGFRHAMPVRQQLKTRTLFNVLGPLINPAHPPLALIGV
                     YSPELVLPIAETLRVLGYQRAAVVHSGGMDEVSLHAPTIVAELHDGEIKSYQLTAEDF
                     GLTPYHQEQLAGGTPEENRDILTRLLQGKGDAAHEAAVAANVAMLMRLHGHEDLQANA
                     QTVLEVLRSGSAYDRVTALAARG"
     gene            complement(5126..6688)
                     /gene="trpE"
                     /locus_tag="ACHOSC_RS12255"
                     /old_locus_tag="pgaptmp_002451"
     CDS             complement(5126..6688)
                     /gene="trpE"
                     /locus_tag="ACHOSC_RS12255"
                     /old_locus_tag="pgaptmp_002451"
                     /EC_number="4.1.3.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004049 - anthranilate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anthranilate synthase component I"
                     /protein_id="HGU4548378.1"
                     /translation="MQTQKPTLELLTCEGAYRDNPTALFHQLCGDRPATLLLESADID
                     SKDDLKSLLLVDSALRITALGDTVTIQALSGNGEALLTLLDNALPAGMENEQLPNCRV
                     LRFPPVSPLLDEDARLCSLSVFDAFRLLQNMLNVPKEEREAMFFGGLFSYDLVAGFED
                     LPQLSAENNCPDFCFYLAETLMVIDHQKKSTRIQASLFAPNEEEKQRLTARLNDLRQQ
                     LTETAPPLPVVSVPHMRCECNQSDEEFGGVVRLLQKAIRAGEIFQVVPSRRFSLPCPS
                     PLAAYYVLKKSNPSPYMFFMQDNDFTLFGASPESSLKYDATSRQIEIYPIAGTRPRGR
                     RADGSLDRDLDSRIELEMRTDHKELSEHLMLVDLARNDLARICTPGSRYVADLTKVDR
                     YSYVMHLVSRVVGELRHDLDALHAYRACMNMGTLSGAPKVRAMQLIAEAEGRRRGSYG
                     GAVGYFTAHGDLDTCIVIRSALVENGIATVQAGAGVVLDSVPQSEADETRNKARAVLR
                     AIATAHHAQETF"
     misc_feature    complement(6710..6803)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00513"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Trp leader region; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /db_xref="RFAM:RF00513"
     gene            complement(6780..6824)
                     /gene="trpL"
                     /locus_tag="ACHOSC_RS12260"
                     /old_locus_tag="pgaptmp_002452"
     CDS             complement(6780..6824)
                     /gene="trpL"
                     /locus_tag="ACHOSC_RS12260"
                     /old_locus_tag="pgaptmp_002452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trp operon leader peptide"
                     /protein_id="HGU4548379.1"
                     /translation="MKAIFVLKGWWRTS"
     gene            6962..7843
                     /gene="rnm"
                     /locus_tag="ACHOSC_RS12265"
                     /old_locus_tag="pgaptmp_002453"
                     /gene_synonym="trpH"
                     /gene_synonym="yciV"
     CDS             6962..7843
                     /gene="rnm"
                     /locus_tag="ACHOSC_RS12265"
                     /old_locus_tag="pgaptmp_002453"
                     /gene_synonym="trpH"
                     /gene_synonym="yciV"
                     /EC_number="3.1.13.-"
                     /EC_number="3.1.3.97"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNase AM"
                     /protein_id="HGU4548380.1"
                     /translation="MSDTNYAVIYDLHSHTTASDGCLTPEALVHRAVEMRVGTLAITD
                     HDTTAAIAPAREEISRSGLALNLIPGVEISTVWENHEIHIVGLNIDITHPLMCEFLAQ
                     QTERRNQRAQLIAERLEKAQIPGALEGAQRLAQGGAVTRGHFARFLVECGKASSMADV
                     FKKYLARGKTGYVPPQWCTIEQAIDVIHHSGGKAVLAHPGRYNLSAKWLKRLVAHFAE
                     HHGDAMEVAQCQQSPNERTQLAALARQHHLWASQGSDFHQPCPWIELGRKLWLPAGVE
                     GVWQLWEQPQNTTEREL"
     gene            7840..8460
                     /gene="yciO"
                     /locus_tag="ACHOSC_RS12270"
                     /old_locus_tag="pgaptmp_002454"
     CDS             7840..8460
                     /gene="yciO"
                     /locus_tag="ACHOSC_RS12270"
                     /old_locus_tag="pgaptmp_002454"
                     /EC_number="2.7.7.87"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003725 - double-stranded RNA binding
                     [Evidence IEA];
                     GO_function: GO:0061710 - L-threonylcarbamoyladenylate
                     synthase [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonylcarbamoyladenylate synthase"
                     /protein_id="HGU4548381.1"
                     /translation="MSQFFYIHPDNPQQRLINQAVEIVRKGGVIVYPTDSGYALGCKI
                     EDKNAMERICRIRQLPDGHNFTLMCRDLSELSTYSFVDNVAFRLMKNNTPGNYTFILK
                     GTKEVPRRLLQEKRKTIGMRVPSNPIAQALLEALGEPMLSTSLMLPGSEFTESDPEEI
                     KDRLEKQVDLIIHGGYLGQKPTTVIDLTDDKPVVVREGVGDVKPFL"
     gene            8561..9433
                     /gene="rluB"
                     /locus_tag="ACHOSC_RS12275"
                     /old_locus_tag="pgaptmp_002455"
     CDS             8561..9433
                     /gene="rluB"
                     /locus_tag="ACHOSC_RS12275"
                     /old_locus_tag="pgaptmp_002455"
                     /EC_number="5.4.99.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA pseudouridine(2605) synthase RluB"
                     /protein_id="HGU4548382.1"
                     /translation="MSEKLQKVLARAGHGSRREIESIIEAGRVSVDGKIAKLGDRVEV
                     TPGLKIRIDGHLISVRESAEQICRVLAYYKPEGELCTRNDPEGRPTVFDRLPKLRGAR
                     WIAVGRLDVNTCGLLLFTTDGELANRLMHPSREVEREYAVRVFGQVDDAKLRDLSRCV
                     QLEDGPAAFKTIKFSGGEGINQWYNVTLTEGRNREVRRLWEAVGVQVSRLIRVRYGDI
                     PLPKGLPRGGWTELDLAQTNYLRELVELPPETSSKVAVEKDRRRMKANQIRRAVKRHS
                     QVSGGRRSGGRNNG"
     gene            complement(9473..10063)
                     /gene="cobO"
                     /locus_tag="ACHOSC_RS12280"
                     /old_locus_tag="pgaptmp_002456"
     CDS             complement(9473..10063)
                     /gene="cobO"
                     /locus_tag="ACHOSC_RS12280"
                     /old_locus_tag="pgaptmp_002456"
                     /EC_number="2.5.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008817 - corrinoid adenosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cob(I)yrinic acid a,c-diamide
                     adenosyltransferase"
                     /protein_id="HGU4548383.1"
                     /translation="MSDERYQQRQQRVKEKVDARVAQAQDERGIIIVFTGNGKGKTTA
                     AFGTATRAVGHGKKVGVVQFIKGTWPNGERNLLEPHGVEFQVMATGFTWDTQNRESDT
                     AACREVWQHAKRMLADSSLDMVLLDELTYMVAYDYLPLEEVVQALNERPHQQTVIITG
                     RGCHRDILELADTVSELRPVKHAFDAGVKAQIGIDY"
     gene            complement(10060..10818)
                     /gene="yciK"
                     /locus_tag="ACHOSC_RS12285"
                     /old_locus_tag="pgaptmp_002457"
     CDS             complement(10060..10818)
                     /gene="yciK"
                     /locus_tag="ACHOSC_RS12285"
                     /old_locus_tag="pgaptmp_002457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YciK family oxidoreductase"
                     /protein_id="HGU4548384.1"
                     /translation="MHYQPKQDLLNDRIILVTGASDGIGREAAMTYARYGATVILLGR
                     NEEKLRQVASHINEETGRQPQWFILDLLTCTSEDCQQLAQRITVNYPRLDGVLHNAGL
                     LGDVCPMSEQNPQVWQDVMQVNVNATFLLTQALLPLLLKSDAGSLVFTSSSVGRQGRA
                     NWGAYAASKFATEGMMQVLADEYQQRLRVNCINPGGTRTAMRASAFPTEDPQKLKTPA
                     DIMPLYLWLMGDDSRRKTGMTFDAQPGRKPGISQ"
     gene            11038..12087
                     /gene="sohB"
                     /locus_tag="ACHOSC_RS12290"
                     /old_locus_tag="pgaptmp_002458"
     CDS             11038..12087
                     /gene="sohB"
                     /locus_tag="ACHOSC_RS12290"
                     /old_locus_tag="pgaptmp_002458"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protease SohB"
                     /protein_id="HGU4548385.1"
                     /translation="MELLSEYGLFLAKIVTVVLAIAAIAAIIVNVAQRNKRQRGELRV
                     NNLSEQYKEMKEELAAALMDSHQQKQWHKAQKKKHKQEAKAAKAKAKLGEVATDSKPR
                     VWVLDFKGSMDAHEVNSLREEITAVLAAFKPQDQVVLRLESPGGMVHGYGLAASQLQR
                     LRDKNIPLTVTVDKVAASGGYMMACVADKIVSAPFAIVGSIGVVAQMPNFNRFLKSKD
                     IDIELHTAGQYKRTLTLLGENTEEGREKFREELNETHQLFKDFVKRMRPSLDIEQVAT
                     GEHWYGQQAVEKGLVDEINTSDEVILSLMEGREVVNVRYMQRKRLIDRFTGSAAESAD
                     RLLLRWWQRGQKPLM"
     gene            complement(12123..12374)
                     /gene="yciN"
                     /locus_tag="ACHOSC_RS12295"
                     /old_locus_tag="pgaptmp_002459"
     CDS             complement(12123..12374)
                     /gene="yciN"
                     /locus_tag="ACHOSC_RS12295"
                     /old_locus_tag="pgaptmp_002459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YciN family protein"
                     /protein_id="HGU4548386.1"
                     /translation="MNKETQPIDRETLLKEANKIIREHEDTLAGIEATGVTQRNGVLV
                     FTGDYFLDEQGLPTAKSTAVFNMFKHLAHVLSEKYHLVD"
     gene            12754..>15324
                     /gene="topA"
                     /locus_tag="ACHOSC_RS12300"
                     /old_locus_tag="pgaptmp_002460"
     CDS             12754..>15324
                     /gene="topA"
                     /locus_tag="ACHOSC_RS12300"
                     /old_locus_tag="pgaptmp_002460"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000513601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003917 - DNA topoisomerase type I (single
                     strand cut, ATP-independent) activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I DNA topoisomerase"
                     /protein_id="HGU4548387.1"
                     /translation="MGKALVIVESPAKAKTINKYLGSDYVVKSSVGHIRDLPTSGSAA
                     KKSADSTSTKTAKKPKKDERGALVNRMGVDPWHNWEAHYEVLPGKEKVVSELKQLAEK
                     ADHIYLATDLDREGEAIAWHLREVIGGDDARYSRVVFNEITKNAIRQAFNKPGELNID
                     RVNAQQARRFMDRVVGYMVSPLLWKKIARGLSAGRVQSVAVRLVVEREREIKAFVPEE
                     FWEVDASTTTPSGEALALQVTHQNDKPFRPVNKEQTQAAVSLLEKARYSVLEREDKPT
                     TSKPGAPFITSTLQQAASTRLGFGVKKTMMMAQRLYEAGYITYMRTDSTNLSQDAVNM
                     VRGYISDNFGKKYLPESPNQYASKENSQEAHEAIRPSDVNVMAESLKDMEADAQKLYQ
                     LIWHQFVACQMTPAKYDSTTLTVGAGDFRLKARGRILRFDGWTKVMPALRKGDEDRIL
                     PAVNKGDALTLVELTPAQHFTKPPARFSEASLVKELEKRGIGRPSTYASIISTIQDRG
                     YVRVENRRFYAEKMGEIVTDRLEENFRELMNYDFTAQMENSLDQVANHEAEWKAVLDH
                     FFSDFTQQLDKAEKDPEEGGMRPNQMVLTSIDCPTCGRKMGIRTASTGVFLGCSGYAL
                     PPKERCKTTINLVPENEVLNVLEGEDAETNALRAKRRCPKCGTAMDSYLIDPKRKLHV
                     CGNNPTCDGYEIEEGEFRIKGYDGPIVECEKCGSEMHLKMGRFGKYMACTNEECKNTR
                     KILRNGEVAPPKEDPVPLPELPCEKSDAYFVLRDGAAGVFLAANTFPKSRETRAPLVE
                     ELYRFRDRLPEKLRYLADAPQQDPEGNKTMVRFSRKTKQQYVSSEKDGKATGWSAFYV
                     D"
BASE COUNT         3719 a         3909 c         4112 g         3584 t
ORIGIN      
        1 gtttgattat attactgttg ggcggaaaaa tgacgtaagt tgacgctcga ccggggtaag
       61 cgaaacggta aaaagataaa tattaaatga atttaggatt tttccggctt cattaaagaa
      121 agttaaaatg ccgccagcgg aactggcggc tgtgggatta actgcgcgtc gccgctttca
      181 tcggttgtac aaaaactttc agtgccgcca gcattttctc tggctcatta atatgttgct
      241 cgatgatttt aacaatggcc gaaccagaaa tcgcgcccgc agctcctgca tcaatcgctg
      301 cttttacctg atccggggcg gaaataccaa atccctgcaa tggaggtgca gcgttgtact
      361 ctttcagctt cgcaaccaga tgattgaggg gtaacgcggc gcggttttct gcgccggtca
      421 cgcctgctcg tgacagcaaa taggtgtaac cacgaccgta agaggctatc tggcgcagca
      481 ggtcgtcatc ggcatttggc gggcagatga agataggtgc gacattatga cgcaacgcgg
      541 cctggcggaa gggcgcggac tcttcaactg gcacatcggc aaccagcacc gaatcgacgc
      601 cgactttttc gcactgggca taaaactcat caatgccttt gttaaacacc agattggcat
      661 acatcaacag gccaatggga atggtcgggt gtttctggcg aatcagtgcc agcatttcaa
      721 aacattgtgc cggagtcaca cctgccgcaa aggcgcgcag agtggcgttt tgaatcgtcg
      781 ggccatccgc cagtgggtcg gagaagggga tacctaactc cagcgcgtca gcaccggctt
      841 caattagcgt atcgataatt ttcaatgact gctcaatgcc cggatcaccg agcgtgacga
      901 aaggaacgaa tgcgccttct ttgcgctcct tcaactgggc aaacagagat tcgtagcgtt
      961 ccatcagatt tcccctcgtg ctttcaaaat atcgtgaacg gtgaagatgt ctttatcgcc
     1021 gcgaccggaa aggttaacca ccagtagctg ctctttatcc gggttttcgc gcatcatttt
     1081 caacgcatgg gccagggcgt gggaggattc cagcgccggg atgatccctt cgtgcaggca
     1141 cagcgttttg aaggcttcaa gggcttcatc atcggtaata gacacgtaat cagcgcgtcc
     1201 agtgctgtta agatacgcgt gttgtgggcc gacagacggg aaatccagtc cggcggagat
     1261 ggagtaagat tcttcaatct gcccgtcttc ggtttgcatc atcggcgctt tcataccgaa
     1321 atagataccc acgcgaccat gttttagcgg tgcgccgtgc tcgccagttt cgataccgtg
     1381 accacctggc tccacaccaa tcaggccgac gttggtttca ttgatgaaat cagcaaacat
     1441 gccgatggca ttcgaaccgc cgccaacaca ggcgataacg gcatccggca ggcgaccttc
     1501 tctttccaga atctgcgctt tggtttcttc gccaatcatc cgctgaaact cacgcacaat
     1561 ggtcggataa ggatgcgggc cagctgcggt gcccagcata tagtgcgcgg tttcgtaact
     1621 accggaccag tcgcgcagcg cctcgttaca ggcatctttc agcgtcgcgg aaccgctatg
     1681 caccgggatc acttccgcac ccattaagcg catacgaaaa acgttaggcg actggcgttc
     1741 aacgtctttg gcacccatat aaatacggca tttcaggccg agcagggcgc tggcaagggc
     1801 cgacgccacg ccatgctgac cggcaccggt ttcggcgatg atttcggttt tacccatccg
     1861 cttcgccagc aacgcctgcc ccagcacctg gttagtttta tgcgcgccgc cgtgcagcaa
     1921 atcttcacgc ttgagataca gcgtagtgtt cgtcccggct gtaatgttct ggcatttggt
     1981 cagcgcggtt ggacgcccgg catagttttt cagcaggtcg ttgaactgag cctgaaattc
     2041 aggatctttt tgcgcactga caaaagcttc ttccagctgg cgcagagcag gcatcaggat
     2101 ttgtggcacg tacatgccgc caaactcacc aaaatagggg ttaagtaatg ttgtcattgt
     2161 tcctttcctt aatatgcgcg cagcgtctgg aaaaccgagg ccaaaagacg tgcgtctttg
     2221 atgcccggtt gcgactctac agcagaatta aaatcaagtc cggcgcagcc ggtttgtgcc
     2281 gcttccacgc agttatctgc gcctaagccc cccgccagca gaacgttgcc aagcgattga
     2341 ccatttaata gtgaccagtc aaaacgttgc ccgcttccac cctggccgtt gtctaaaaca
     2401 tatttatcaa cgtgctgaaa ctcgcgggcg ggcagggttt caccgacgct taatgctttc
     2461 cagatggcaa catgtgctgg cagagcttca cgcagcgtat cgatatacag ctgttcttca
     2521 ttaccatgca gttgcactgc cgccagcgat aacaccttag ctttgtccac cacatcggca
     2581 atatcgtgat tgcggaacac gccaacatac tgcaacggtg ccgcagccat cacttcctgc
     2641 gcctgttcaa cgttgacgca acgcggtgat gtcgcaacaa aaatcaaccc accgtaaatc
     2701 gcgcccgcgt cataagctgc tttagcatct tgcccacgcg tcaggccaca tactttattc
     2761 tcacccagca acacccggcg cacggcggcg tgcaaatcgt catgggccat caacgccgaa
     2821 ccaatcagaa aaccgttagc gaagtggctt aactcgcgca cctgagcgta agtattgatg
     2881 ccggattcgc tgattaccgt cacgttgtgc cccagtttcg gcgcaagctc gcgggtacgg
     2941 ttgagatcaa tcgacaaatc acgcagatcg cggttgttga tgccaacgac ctttgccccc
     3001 aatgcaatgg cacgctccag ttcctcttca ttactgactt cggtcagcac acccatctcc
     3061 agactgtggg cgacggcggc aagctggcga tattgttcgt catccagtac tgaaagcatt
     3121 aataagcagg catcagcctg gtaatagcgc gccagataga tctggtaagg atcgataatg
     3181 aagtctttac ataaaatcgg ctgcggggcg atttggctga cgatggggag gaaatcaaag
     3241 ctcccctgaa aatatttctc atcagtcagc accgaaattg ccgaagcgta atgtttataa
     3301 atggcggcaa tgcgtgctgg atcgaaatca tcacggatca cgccttttga cggcgacgat
     3361 ttcttgcact ccagaataaa cgccgtgcgc gcaccctgta gcgcatcata aaaatgtcgc
     3421 gtgctcggct gaacctcatt ctgaaaactg gccagcggtt gctgctgttt gcgggtttct
     3481 acccaaatcg ccttgtctgc gacgattttc gctaaaacgg tttgcatcat ttaccctcgt
     3541 gccgccagtg cggtaactct gtcgtaagcg gaaccactgc gcagtacctc aagaacggtt
     3601 tgcgcattgg cttgcagatc ttcatggcca tgcaggcgca ttaacatggc gacgttcgca
     3661 gcgacggctg cttcatgggc ggcgtcgcct ttaccttgta acaagcgtgt taaaatgtca
     3721 cggttttctt ccggtgttcc gcctgccagt tgctcctggt ggtagggagt caggccaaaa
     3781 tcttcagcgg tcaattgata gctcttaatt tcgccgtcat gcagctcggc aacgattgtc
     3841 ggcgcgtgta atgaaacttc atccatcccg ccgctgtgca ccaccgccgc gcgttgatac
     3901 cccagcacgc gcaaggtttc ggcaatcggc agcaccagtt ccggactata aacaccaatt
     3961 aacgccagcg gcggatgcgc cgggttaatc aatggcccca gcacattgaa cagggtgcgg
     4021 gttttcagtt gctggcgaac cggcatcgcg tggcggaatc cggtgtgata cttcggcgca
     4081 aagaggaaac atacacctaa ctcatccagc gcctggcgcg atttatcggc gttcatatca
     4141 agattaatac cgaacgccgc cagcagatcg gacgaaccag atttactgga gacgctacgg
     4201 ttgccgtgtt tcgccacttt cagcccacag gccgcggcga caaacgcact ggcggtagaa
     4261 atattgatac tgttgctgcc gtcaccgcca gtaccgacga tatcagcaaa cagataatcc
     4321 gggcgcggga acggcgcggc gttttccagt agcgcggttg ctgctccggc gatctcgttc
     4381 gggtgctcac cgcgaatttt catgctcacc agcgccgccg ccagttgttc cggcttcagc
     4441 tcgccacgca ccaccgctga aaacagctgg tggctttctt gttggctaag cgtctgcgcc
     4501 tgatacagtt tttccagaat cggttgcagc gtgttggctg gctctagttt ctgctgcgcc
     4561 caggccagcg tttgttccag caggcgagcg ccctgggtgg tgagaatgga ttccggatgg
     4621 aactggaatc cacaaacgcg atccgcatcg tgacgtactg ccatcaccat gccattaaaa
     4681 tgggcgttga tggttaaacc ggccggaatg ttactgccaa ccagcgagtg ataacgcgcc
     4741 accggcagcg ggtttgttaa tccggcaaac atcgcctgac cgtcatgttc aatgctcgac
     4801 gctttaccgt gaagaatttc gcccgcctga ccgacatagc ccccgtaagc ttcgacaatt
     4861 gcctgatgtc cgaggcaaat gccaataatg ggcagcttgc cacgcaagcg ggtgaggagt
     4921 tccggcatac aaccggcttc gctcggcaca ccggggccag gagaaagcat cagtaccgga
     4981 ttgctcatcg tcgccaggcg ttcaattaag gtctgcgccg gaatatggtt gcggtaaatc
     5041 accacattat gcccattgct gcgcaactga tctgccaggt tgtacgtaaa agagtcgata
     5101 ttatcgagca gcagaatgtc agccatcaga aagtctcctg tgcatgatgc gcggtggcaa
     5161 tagcgcgcag tacagcgcgg gctttattac gggtttcgtc ggcttccgac tgcggaacag
     5221 aatcaaggac tacgccagca ccggcttgca cggtggcgat accgttttcc accagcgccg
     5281 agcggatcac aatgcaggtg tcgagatcgc catgcgcggt gaaataacct accgcgccgc
     5341 cgtagctgcc gcggcgacga ccttccgcct cggcaattaa ctgcatagcg cgtactttcg
     5401 gcgcaccact taacgttccc atattcatac aggcgcggta agcgtgcagg gcgtcgagat
     5461 cgtggcgcag ctcaccgacc acgcgggaga ccaagtgcat cacgtaagag taacggtcaa
     5521 ctttggtgag gtcggcgacg tagcggctgc cgggggtgca aatgcgtgcc agatcattac
     5581 gggcgagatc caccagcatc agatgttcag aaagctcttt atgatcggta cgcatttcca
     5641 gttcgatgcg gctgtcgagg tctctgtcca gtgaaccatc ggcacgacga ccgcgcggtc
     5701 gtgttccggc aatcgggtag atctcaatct ggcggctggt ggcgtcatac ttgagcgaac
     5761 tttccggcga cgcgccaaac agggtgaaat cattatcctg cataaaaaac atgtacgggc
     5821 tgggattact ctttttcagc acgtaatagg ccgccagcgg tgacgggcag ggcagagaga
     5881 aacggcgaga tggcaccacc tggaaaattt ctccggcgcg aatcgctttt tgcaacaaac
     5941 gcactacgcc accgaactct tcatcgctct ggttacattc acaacgcata tgcggcacgg
     6001 aaaccaccgg cagcggtggc gcggtttcgg tcagttgctg gcgaagatcg ttcaggcgag
     6061 cagtgagacg ttgtttttct tcttcattcg gagcaaacag gctggcctga atgcgggtgc
     6121 tttttttctg atggtcaatc accatcagcg tttcagcgag ataaaaacag aaatcaggac
     6181 agttattttc cgctgacaat tgcggtaaat cttcaaaccc cgccacaagg tcataagaga
     6241 acaggccgcc gaagaacatt gcttctcgtt cttccttcgg tacattcagc atattctgca
     6301 ataagcggaa agcgtcaaaa accgaaaggg agcataagcg agcgtcttca tccagcagtg
     6361 gactgacagg ggggaagcgc agcacacggc agtttggtaa ttgttcattt tccatacccg
     6421 caggcagggc gttatccagt agtgtcagca gggcttcgcc gttgccggaa agtgcctgga
     6481 ttgtgacagt gtcacctaaa gctgtaatgc gcagcgcact gtctaccagc agcaggcttt
     6541 ttaaatcatc tttgctgtcg atatctgcgg attccagcag cagcgttgcc ggacgatccc
     6601 cacacaactg gtgaaaaagc gcggtgggat tgtcgcgata agcgccttcg caggttagca
     6661 gttcgagagt cggtttttgt gtttgcattg ttattctcta attttgttca aaaaaaagcc
     6721 cgctcattag gcgggctggg tatctgattg ctttacgcat ggtgaataca ctgcccgttt
     6781 caggaagtgc gccaccaacc tttcaggacg aaaattgctt tcattgtcga tacccttttt
     6841 acgtgaactt gcgtactagt taactagttc gatgattaat tgtcaatagc tcatttcaga
     6901 atatttgcca gaaccgttat gatgtcggcg caaaaaacat tatccagaac gggagtgcgc
     6961 cttgagcgac acgaattatg cagtgattta cgacctgcac agccatacca cagcttccga
     7021 tggctgcctg acgccagaag cattggtgca ccgtgcagtc gagatgcgcg tcggcaccct
     7081 ggcgatcacc gaccatgaca ccacagctgc aatcgcgcca gcaagagagg aaatttcacg
     7141 ttcgggactg gcgttgaatc ttattcccgg cgtggaaatt tccacggtct gggaaaatca
     7201 tgaaattcat attgtcgggc tgaatattga tattactcat ccgctaatgt gtgagttcct
     7261 cgcgcagcag acagaacggc gcaatcagcg ggcgcagctg attgccgaaa gacttgaaaa
     7321 agcgcaaata cctggggcgc tggaaggcgc acaacgactg gcgcagggcg gcgcagtgac
     7381 gcgcggtcat tttgcgcgtt tccttgttga gtgcggcaaa gccagttcaa tggcggatgt
     7441 ctttaaaaag tatctggcgc gcgggaaaac cggatacgtt ccgccacagt ggtgtacaat
     7501 agaacaagct attgatgtca ttcatcattc tggcggtaag gcggtgctag ctcatcctgg
     7561 gcggtacaat cttagtgcta aatggctgaa aagattggtt gcgcattttg ccgaacacca
     7621 cggtgacgcg atggaagtcg cgcagtgcca gcaatcgccc aatgaacgta cccagctggc
     7681 ggcccttgcg cgtcagcatc atttatgggc atcacaagga tctgattttc atcagccatg
     7741 cccgtggatc gaactgggtc gtaaactctg gctgcccgca ggcgttgaag gcgtctggca
     7801 gctatgggaa cagccgcaga acaccacaga gagggaatta tgagccagtt tttttatatt
     7861 catcctgata acccacagca acgtctgatc aaccaggcgg tggagatcgt gcgtaaaggc
     7921 ggggtgattg tttatccaac tgattccggc tatgcgctcg gctgtaaaat tgaagataaa
     7981 aacgcgatgg agcgtatttg tcgtattcgc cagctgccgg acggtcacaa ctttaccctg
     8041 atgtgtcgcg atctttctga actctcgacc tattcatttg ttgataacgt tgcgtttcgt
     8101 ttaatgaaaa acaacacgcc gggcaactat accttcatct tgaaggggac gaaggaagtg
     8161 ccacgccgtc tgttgcagga aaaacgcaaa accatcggta tgcgtgtgcc ttctaacccg
     8221 attgcgcagg cgttgcttga agcactgggc gaaccgatgc tttccacttc gctaatgctg
     8281 ccaggcagcg aatttaccga atccgatccc gaagaaatta aagaccgtct ggaaaaacag
     8341 gtagatttga ttattcacgg cggctatctc gggcagaaac cgacaacggt tattgatctt
     8401 accgatgata agccggtcgt ggtgcgtgaa ggcgtaggtg atgtgaagcc tttcttataa
     8461 gtgcgtaact ctgtatacta cgcggcctta aaatggcgct atacggcgcc attgattctc
     8521 ctgttcgacg cctgtgaagg cgacactaaa aggaagctct atgagcgaaa aattacagaa
     8581 agtgctggcc cgcgctggcc acggctcccg ccgtgaaatc gaatctatta ttgaagccgg
     8641 tcgtgtgagt gttgatggca aaattgctaa actcggcgat cgcgttgaag ttacccctgg
     8701 gctgaaaatt cgtatcgatg gtcatctgat ttcggtacgt gagtccgctg aacaaatttg
     8761 tcgcgttctg gcctattaca aaccggaagg tgagttgtgt acccgtaacg atccggaagg
     8821 gcgtccgacg gtgtttgacc gtctgccaaa gctgcgtggc gcacgctgga ttgccgtagg
     8881 tcgtctggac gttaacacct gtggtctgct gctgttcacc accgatggtg aactggcaaa
     8941 ccgtttaatg catccaagcc gtgaagttga acgtgaatac gccgtgcgtg tatttggtca
     9001 ggttgacgac gcgaaactgc gtgatttgag tcgttgcgtg caactggaag atggtccggc
     9061 agcttttaaa accatcaagt tcagcggcgg cgaagggatc aaccagtggt acaacgtgac
     9121 tctgaccgaa ggccgtaacc gtgaagttcg tcgtctgtgg gaagcggttg gtgtgcaggt
     9181 gagccgcctg atccgtgttc gttacggtga tatcccactg ccaaaaggtc tgccacgcgg
     9241 tggctggacg gagctggacc tcgcccagac taactatctg cgcgaactgg tggagctacc
     9301 gccagaaacc agctctaaag tcgctgtaga aaaagaccgt cgtcgcatga aggcgaatca
     9361 gattcgtcgt gcggtgaaac gtcacagtca ggtgagcggc ggtcgtcgtt ctggcggacg
     9421 taataacggt taatcaaagt atgccggacg tcatatccgg catttttaca gattagtaat
     9481 caatccctat ctgcgctttt acaccggcat caaacgcatg tttgacgggg cgtaattcac
     9541 ttaccgtatc tgccagttca agaatatccc gatgacaacc acgacccgtg ataatcaccg
     9601 tctgttgatg tggacgttca tttaacgcct gcaccacttc ttccagtggc aaatagtcat
     9661 acgccaccat atacgtcagt tcatcaagca aaaccatatc cagtgaggaa tcagcaagca
     9721 tccgctttgc atgttgccag acttcgcggc aggcggcggt atcagactcg cggttttgtg
     9781 tatcccaggt aaagcccgtt gccatcacct gaaactcaac gccatgtggc tccagcagat
     9841 tgcgttcgcc attaggccag gtgcctttaa taaactgcac gacacctact ttttttccgt
     9901 gaccaactgc gcgtgttgcc gtaccaaatg ccgcggtggt tttgcctttt ccattgccgg
     9961 taaagacgat gataataccg cgttcatcct gggcctgggc cacacgagca tctacttttt
    10021 ctttcactcg ctgctgacgc tgttggtagc gttcatcact cattgggaaa ttcctggttt
    10081 acggcccggt tgggcgtcaa aggtcatgcc ggttttacgg cggctgtcat cgcccatcag
    10141 ccagaggtag agcggcatga tatcagcggg tgttttaagt ttctgtggat cttcggtcgg
    10201 gaaggcgctg gcacgcattg cggtgcgcgt accgcctggg ttaatgcagt tgacgcgcag
    10261 gcgctgctga tattcatcgg ccagtacctg catcatccct tcggtggcaa atttcgacgc
    10321 tgcatatgca ccccagttag ctcgtccctg acgtccaacg cttgatgaag taaagaccag
    10381 tgaaccggcg tccgatttga gtaataaagg aagcagcgcc tgggtgagca aaaaggtggc
    10441 attaacgttg acctgcatga cgtcctgcca gacctgcgga ttttgttcgc tcattgggca
    10501 aacatcgccg agcaatccgg cattatgcaa aacaccatcc agacgcggat aattaacggt
    10561 aatgcgctgt gccagttgtt ggcaatcttc ggacgtgcag gtcagcaaat cgagaataaa
    10621 ccactgtggc tgacgtccag tttcttcgtt tatgtggctg gctacctgac gtaatttttc
    10681 ttcattacgg cccaacagaa tcactgtcgc accatagcgt gcatacgtca tcgcggcttc
    10741 acggccaatc ccatcgctgg ctcccgtcac caggataatg cgatcattga gtaaatcttg
    10801 ttttggctgg taatgcatgg ctactcctca acgacgttgt ctgtcgcgct gccagtgtac
    10861 tttatgactt tggggcttta tgcctgaaac agaagggcat ttcaatcagc ctggggcgat
    10921 aacaccgcaa aatggatact ttgtcatact ttcgctgcaa taacatctct gcgagacggc
    10981 ttaacatgcc tgttgtaaac tgtgagccaa agcgttgttt aaccaaggtg gggactcgtg
    11041 gaattgttgt ctgaatatgg tttgtttttg gcgaaaatcg ttaccgttgt gctagcgatt
    11101 gcggcgattg ccgccattat tgtcaatgtt gctcaacgta ataaacgcca gcgtggcgag
    11161 ttacgggtca acaatctcag cgaacagtat aaggagatga aagaagaact ggccgcggcg
    11221 ctgatggact cacatcagca aaaacagtgg cacaaagcgc agaagaaaaa gcacaagcaa
    11281 gaagcgaaag cagcaaaagc gaaagccaaa ctgggcgagg tggcaactga cagtaaacct
    11341 cgcgtctggg tgctggattt taaaggcagc atggacgccc atgaagtgaa ctcgctacgt
    11401 gaagagataa cggcggtact cgcagcattc aaaccgcagg atcaggttgt gctccgtctg
    11461 gaaagccctg gtggcatggt gcatggttac gggttggcgg cttcgcagct gcagcgtctg
    11521 cgtgataaaa acattccttt aactgttacg gtagacaaag tcgctgccag cggcggttac
    11581 atgatggcct gtgtggcgga caaaattgtt tccgcaccgt ttgctattgt gggttccatt
    11641 ggggtggtgg cgcaaatgcc caactttaac cgcttcctga aaagcaaaga tattgatatc
    11701 gaactgcaca ctgccgggca gtataagcgt actctgacct tgctgggtga aaataccgaa
    11761 gaagggcggg agaaattccg cgaagagttg aacgaaacgc atcagttgtt taaagatttt
    11821 gtgaagcgta tgcgtccgtc tctggatatt gaacaggtgg caacgggtga acactggtac
    11881 ggacaacagg cggtagagaa aggcctggtt gatgaaatca acaccagtga tgaagttatt
    11941 cttagcctga tggaaggccg tgaagtggtt aatgtacgct atatgcagcg taaacgactc
    12001 attgaccgat tcaccggcag cgcggcagag agcgccgatc gattgttgct acgctggtgg
    12061 cagcggggtc aaaagccatt gatgtaaaag acaaacgcga ggctaagacc tcgcgttttg
    12121 ctttaatcaa ccagatgata tttttctgaa agcacatggg ccaggtgttt gaacatatta
    12181 aacaccgcgg tgcttttggc tgttggcaat ccttgttcat ctaaaaagta gtcgccggta
    12241 aataccagca cgccattacg ctgcgtgacg ccggtggctt caatccctgc gagcgtatcc
    12301 tcatgctcac gaatgatttt gttggcctct ttcaacagcg tttcgcggtc gatgggttgt
    12361 gtctctttgt tcattattta ctccttaaac aaggacatta gtctacgcca ggcatggctt
    12421 gcagacaaat ataccacgct ggtggcaaga gcgccttact ggtaactttg gattttgcat
    12481 gctaataaag ttgcgtatcg gattttatca ggtacagtgt gacgctttcg tcaatctggc
    12541 aatagatttg cttgacattc gaccaaaatt ccgtcgtgct atagcgcctg taggccaaga
    12601 cctgttaact cagtcacctg aattttcgtg aacagagtca cgacaagggg ttgatatccg
    12661 cagagaacga gtccatatcg gtaactcgtt gccagtggaa ggtttatcaa cgtgcgacgc
    12721 attcctggaa gaatcaaatt aggtaaggtg aatatgggta aagctcttgt catcgttgag
    12781 tccccggcaa aagccaaaac gatcaacaag tatctgggta gtgactacgt ggtgaaatcc
    12841 agcgtcggtc acatccgcga tttgccgacc agtggctcag ctgccaaaaa gagtgccgac
    12901 tctacctcca ccaagacggc taaaaagcct aaaaaggatg aacgtggcgc tctcgtcaac
    12961 cgtatggggg ttgacccgtg gcacaattgg gaggcgcact atgaagtgtt gcctggtaaa
    13021 gagaaggtcg tctctgaact gaaacaactg gctgaaaaag ccgaccacat ctatctcgca
    13081 accgaccttg accgcgaagg ggaagccatt gcatggcacc tgcgggaagt gattgggggt
    13141 gatgatgcgc gctatagccg agtggtgttt aacgaaatta ctaaaaacgc gatccgccag
    13201 gcatttaata aaccgggtga gctgaatatt gatcgtgtta atgcccagca ggcgcgtcgc
    13261 tttatggacc gcgtggtggg gtatatggtt tcgccgctgc tatggaaaaa gatcgctcgt
    13321 ggtctgtctg ccggtcgtgt gcagtcggtg gcggttcgcc tggtggtcga gcgtgagcgt
    13381 gaaattaaag cgttcgtgcc ggaagagttc tgggaagtcg atgccagcac gaccacgcca
    13441 tctggtgaag cgttggcgtt acaggtgact catcagaacg acaaaccgtt ccgtccggtc
    13501 aacaaagaac aaactcaggc tgcggtaagt ctgctggaaa aagcgcgcta cagcgtgctg
    13561 gaacgtgaag acaaaccgac aaccagtaaa cctggcgctc cttttattac ctctacgctg
    13621 caacaagctg ccagcacccg tcttggattt ggcgtgaaaa aaaccatgat gatggcgcag
    13681 cgtttgtatg aagcaggcta tatcacttac atgcgtaccg actccactaa cctgagtcag
    13741 gacgcggtaa atatggttcg cggttatatc agcgataatt ttggtaagaa atatctgccg
    13801 gaaagtccga atcagtacgc cagcaaagaa aactcacagg aagcgcacga agcgattcgt
    13861 ccttctgacg tcaatgtgat ggcggaatcg ctgaaggata tggaagcaga tgcgcagaaa
    13921 ctgtaccagt taatctggca tcagttcgta gcctgccaga tgaccccagc gaaatatgac
    13981 tccacgacgc tgaccgttgg tgcgggcgat ttccgcctga aagcacgcgg tcgtattttg
    14041 cgttttgatg gctggacaaa agtgatgcct gcgttgcgta aaggcgatga agatcgcatc
    14101 ttaccagcag ttaataaagg cgatgctctg acgctcgttg aacttacacc agcccagcac
    14161 tttaccaagc cgccagcccg tttcagtgaa gcatcgctgg ttaaagagct ggaaaaacgt
    14221 ggtatcggtc gtccgtctac ttatgcgtcg atcatttcga ccattcagga tcgtggctac
    14281 gtgcgagtag aaaatcgtcg tttctatgcg gaaaaaatgg gcgaaatcgt caccgatcgc
    14341 cttgaagaaa atttccgcga gttaatgaac tacgacttta ccgcgcagat ggaaaacagc
    14401 ctcgaccagg tggcaaatca cgaagcagag tggaaagctg tactggatca cttcttctcg
    14461 gatttcaccc agcagttaga taaagctgaa aaagatccgg aagagggtgg tatgcgcccg
    14521 aaccagatgg ttctgaccag cattgactgc ccgacttgtg gtcgcaaaat ggggattcgc
    14581 acagcgagca ccggggtatt ccttggctgt tctggctatg cgctgccgcc gaaagagcgt
    14641 tgcaaaacca ccattaacct ggtgccggaa aacgaagtgc tgaacgtgct ggaaggcgaa
    14701 gatgctgaaa ccaacgcgct gcgcgcaaaa cgtcgttgcc cgaaatgcgg cacggcgatg
    14761 gacagctatc tcatcgatcc gaaacgtaag ttgcatgtct gtggtaataa cccaacctgc
    14821 gacggttacg agatcgaaga gggcgaattc cgcattaaag gttatgacgg cccgatcgtt
    14881 gagtgtgaaa aatgtggctc tgaaatgcac ctgaaaatgg ggcgtttcgg taaatacatg
    14941 gcctgcacca acgaagagtg taaaaacaca cgtaagattt tacgtaacgg cgaagtggca
    15001 ccaccgaaag aagatccggt gccattacct gagctgccgt gcgaaaaatc agatgcttat
    15061 ttcgtgctgc gtgacggtgc tgccggtgtg ttcctggctg ccaacacttt cccgaaatcg
    15121 cgtgaaacgc gtgcgccact ggtggaagag ctttatcgct tccgcgaccg tctgccggaa
    15181 aaactgcgtt atctggccga tgcgccacag caggatccgg aaggtaataa gaccatggtt
    15241 cgctttagcc gtaaaaccaa acagcaatat gtctcttcgg aaaaagacgg aaaggcgact
    15301 ggctggtcag cattttatgt tgat
//
LOCUS       DAZKHF020000096        15305 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2201S31241
            SAMN43267800-rid25106413.denovo.097, whole genome shotgun sequence.
ACCESSION   DAZKHF020000096 DAZKHF020000000
VERSION     DAZKHF020000096.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267800
            Sequence Read Archive: SRR30307263
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 15305)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15305)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 15305)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350581.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350581.2
            Genome Coverage       :: 151x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:21:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,652
            CDSs (total)                      :: 5,557
            Genes (coding)                    :: 5,320
            CDSs (with protein)               :: 5,320
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 237
            CDSs (without protein)            :: 237
            Pseudo Genes (ambiguous residues) :: 0 of 237
            Pseudo Genes (frameshifted)       :: 102 of 237
            Pseudo Genes (incomplete)         :: 130 of 237
            Pseudo Genes (internal stop)      :: 52 of 237
            Pseudo Genes (multiple problems)  :: 41 of 237
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15305
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267800-rid25106413.denovo.097"
                     /serotype="O 26:H11"
                     /isolate="2201S31241"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-01"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            complement(157..1068)
                     /gene="gsiD"
                     /locus_tag="ACHOSC_RS12305"
                     /old_locus_tag="pgaptmp_002461"
     CDS             complement(157..1068)
                     /gene="gsiD"
                     /locus_tag="ACHOSC_RS12305"
                     /old_locus_tag="pgaptmp_002461"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione ABC transporter permease GsiD"
                     /protein_id="HGU4548388.1"
                     /translation="MRLFNWRRQAVLNAMPLVKPDQVRTPWHEFWRRFRRQHMAMTAA
                     LFVILLIVVAIFARWIAPYDAENYFDYDNLNNGPSLQHWFGVDSLGRDIFSRVLVGAQ
                     ISLAAGVFAVFIGAAIGTLLGLLAGYYEGWWDRLIMRICDVLFAFPGILLAIAVVAVL
                     GSGIANVIIAVAIFSIPAFARLVRGNTLVLKQQTFIESARSIGASDMTILLRHILPGT
                     VSSIVVFFTMRIGTSIISAASLSFLGLGAQPPTPEWGAMLNEARADMVIAPHVAVFPA
                     LAIFLTVLAFNLLGDGLRDALDPKIKG"
     gene            complement(1071..1991)
                     /gene="gsiC"
                     /locus_tag="ACHOSC_RS12310"
                     /old_locus_tag="pgaptmp_002462"
     CDS             complement(1071..1991)
                     /gene="gsiC"
                     /locus_tag="ACHOSC_RS12310"
                     /old_locus_tag="pgaptmp_002462"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione ABC transporter permease GsiC"
                     /protein_id="HGU4548389.1"
                     /translation="MLNYVIKRLLGLIPTLFIVSVLVFLFVHMLPGDPARLIAGPEAD
                     AQVIELVRQQLGLDQPLYHQFWHYISNAVQGDFGLSMVSRRPVADEIASRFMPTLWLT
                     ITSMVWAVIFGMAAGIIAAVWRNRWPDRLSMTIAVSGISFPAFALGMLLIQVFSVELG
                     WLPTVGADSWQHYILPSLTLGAAVAAVMARFTRASFVDVLSEDYMRTARAKGVSETWV
                     VLKHGLRNAMIPVVTMMGLQFGFLLGGSIVVEKVFNWPGLGRLLVDSVEMRDYPVIQA
                     EILLFSLEFILINLVVDVLYAAINPAIRYK"
     gene            complement(2009..3547)
                     /gene="gsiB"
                     /locus_tag="ACHOSC_RS12315"
                     /old_locus_tag="pgaptmp_002463"
     CDS             complement(2009..3547)
                     /gene="gsiB"
                     /locus_tag="ACHOSC_RS12315"
                     /old_locus_tag="pgaptmp_002463"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706707.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0034634 - glutathione transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0034775 - glutathione transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione ABC transporter substrate-binding
                     protein GsiB"
                     /protein_id="HGU4548390.1"
                     /translation="MARAVHRSGLVALGIATALMASCAFAAKDVVVAVGSNFTTLDPY
                     DANDTLSQAVAKSFYQGLFGLDKEMKLKNVLAESYTVSDDGITYIVKLREGIKFQDGT
                     DFNAVAVKANLDRASDPANHLKRYNLYKNIAKTEAIDPTTVKITLKQPFSAFINILAH
                     PATAMISPAALEKYGKEIGFHPVGTGPYELDTWNQTDFVKVKKFAGYWQPGLPKLDSI
                     TWRPVADNNTRAAMLQTGEAQFAFPIPYEQATLLEKNKNIELMASPSIMQRYISMNVT
                     QKPFDNPKVREALNYAINRPALVKVAFAGYATPATGVVPPSIAYAQSYKPWPYDPVKA
                     RELLKEAGYPNGFSTTLWSSHNHSTAQKVLQFTQQQLAQVGIKAQVTAMDAGQRAAEV
                     EGKGQKESGVRMFYTGWSASTGEADWALSPLFASQNWPPTLFNTAFYSNKQVDDFLAQ
                     ALKTNDPAEKTRLYKAAQDIIWQESPWIPLVVEKLVSAHSKNLTGFWIMPDTGFSFED
                     ADLQ"
     gene            complement(3567..5438)
                     /gene="gsiA"
                     /locus_tag="ACHOSC_RS12320"
                     /old_locus_tag="pgaptmp_002464"
     CDS             complement(3567..5438)
                     /gene="gsiA"
                     /locus_tag="ACHOSC_RS12320"
                     /old_locus_tag="pgaptmp_002464"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415350.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione ABC transporter ATP-binding protein
                     GsiA"
                     /protein_id="HGU4548391.1"
                     /translation="MPHSDELDAGNVLAVENLNIAFMQDQQKIAAVRNLSFSLQRGET
                     LAIVGESGSGKSVTALALMRLLEQAGGLVQCDKMLLQRRSREVIELSEQSAAQMRHVR
                     GADMAMIFQEPMTSLNPVFTVGEQIAESIRLHQNASREEAMVEAKRMLDQVRIPEAQT
                     ILSRYPHQLSGGMRQRVMIAMALSCRPAVLIADEPTTALDVTIQAQILQLIKVLQKEM
                     SMGVIFITHDMGVVAEIADRVLVMYQGEAVETGTVEQIFHAPQHPYTRALLAAVPQLG
                     AMKGLDYPRRFPLISLEHPAKQAPPIEQKTVVDGEPVLRVRNLVTRFPLRSGLLNRVT
                     REVHAVEKVSFDLWPGETLSLVGESGSGKSTTGRALLRLVESQGGEIIFNGQRIDTLS
                     PGKLQALRRDIQFIFQDPYASLDPRQTIGDSIIEPLRVHGLLPGKDAAARVAWLLERV
                     GLLPEHAWRYPHEFSGGQRQRICIARALALNPKVIIADEAVSALDVSIRGQIINLLLD
                     LQRDFGIAYLFISHDMAVVERISHRVAVMYLGQIVEIGPRRAVFENPQHPYTRKLLAA
                     VPVAEPSRQRPQRVLLSDDLPSNIHLRGEEVAAVSLQCVGPGHYVAQPQSEYAFMRR"
     gene            complement(5425..6390)
                     /gene="iaaA"
                     /locus_tag="ACHOSC_RS12325"
                     /old_locus_tag="pgaptmp_002465"
     CDS             complement(5425..6390)
                     /gene="iaaA"
                     /locus_tag="ACHOSC_RS12325"
                     /old_locus_tag="pgaptmp_002465"
                     /EC_number="3.4.19.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-aspartyl-peptidase"
                     /protein_id="HGU4548392.1"
                     /translation="MGKAVIAIHGGAGAISRAQMSLQQELRYIEALSAIVETGQKMLE
                     AGESALDVVTEAVRLLEECPLFNAGIGAVFTRDETHELDACVMDGNTLKAGAVAGVSH
                     LRNPVLAARLVMEQSPHVMMIGEGAENFAFARGMERVSPEIFSTPLRYEQLLAARKEG
                     ATVLDHSGAPLDEKQKMGTVGAVALDLDGNLAAATSTGGMTNKLPGRVGDSPLVGAGC
                     YANNASVAVSCTGTGEVFIRALAAYDIAALMDYGGLSLAEACERVVMEKLPALGGSGG
                     LIAIDHEGNVALPFNTEGMYRAWGYAGDTPTTGIYREKGDTVATQ"
     gene            6594..7829
                     /gene="moeA"
                     /locus_tag="ACHOSC_RS12330"
                     /old_locus_tag="pgaptmp_002466"
     CDS             6594..7829
                     /gene="moeA"
                     /locus_tag="ACHOSC_RS12330"
                     /old_locus_tag="pgaptmp_002466"
                     /EC_number="2.10.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0032324 - molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin molybdotransferase MoeA"
                     /protein_id="HGU4548393.1"
                     /translation="MEFTTGLMSLDTALNEMLSRVTPLTAQETLPLVQCFGRILASDV
                     VSPLDVPGFDNSAMDGYAVRLADIASRQPLPVAGKSFAGQPYHGEWPAGTCIRIMTGA
                     PVPEGCEAVVMQEQTEQTDNGVRFTAEVRSGQNIRRRGEDISAGAVVFPAGTRLTTAE
                     LPVIASLGIAEVPVIRKVRVALFSTGDELQLPGQPLGDGQIYDTNRLAVHLMLEQLGC
                     EVINLGIIRDDPHALRAAFIEADSQADVVISSGGVSVGEADYTKTILEELGEIAFWKL
                     AIKPGKPFAFGKLSNSWFCGLPGNPVSATLTFYQLVQPLLAKLSGNTASGLPARQRVR
                     TASRLKKTPGRLDFQRGVLQRNADGELEVTTTGHQGSHIFSSFSLGNCFIVLERDRGN
                     VEVGEWVEVEPFNALFGGL"
     gene            7829..8578
                     /gene="moeB"
                     /locus_tag="ACHOSC_RS12335"
                     /old_locus_tag="pgaptmp_002467"
     CDS             7829..8578
                     /gene="moeB"
                     /locus_tag="ACHOSC_RS12335"
                     /old_locus_tag="pgaptmp_002467"
                     /EC_number="2.7.7.80"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008641 - ubiquitin-like modifier
                     activating enzyme activity [Evidence IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-synthase adenylyltransferase MoeB"
                     /protein_id="HGU4548394.1"
                     /translation="MTELSDQEMLRYNRQIILRGFDFDGQEALKDSRVLVVGLGGLGC
                     AASQYLASAGVGNLTLLDFDSVSLSNLQRQTLHSDATVGQPKVESARDAMTRINPHIA
                     ITPVNALLDDAELAAMIAKHDLVLDCTDNVAVRNQLNAGCFAAKVPLVSGAAIRMEGQ
                     ITVFTYQDGEPCYRCLSRLFGENALTCVEAGVMAPLIGVIGSLQAMEAIKLLAGYGKP
                     ASGKIVMYDAMTCQFREMKLMRNPGCEVCGQ"
     gene            complement(8654..9316)
                     /gene="fsa"
                     /locus_tag="ACHOSC_RS12340"
                     /old_locus_tag="pgaptmp_002468"
     CDS             complement(8654..9316)
                     /gene="fsa"
                     /locus_tag="ACHOSC_RS12340"
                     /old_locus_tag="pgaptmp_002468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415346.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016832 - aldehyde-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-6-phosphate aldolase"
                     /protein_id="HGU4548395.1"
                     /translation="MELYLDTSDVVAVKALSRIFPLAGVTTNPSIIAAGNKPLDVVLP
                     QLHEAMGGQGRLFAQVMATTAEGMVNDARKLRSIIADIVVKVPVTAEGMAAIKMLKAE
                     GIPTLGTAVYGAAQGLLSALAGAEYVAPYVNRIDAQGGSGIQTVTDLHQLLKMHAPQA
                     KVLAASFKTPRQALDCLLAGCESITLPLDVAQQMISYPAVEAAVAKFEQDWLGAFGRT
                     SI"
     gene            9447..10346
                     /gene="ybiY"
                     /locus_tag="ACHOSC_RS12345"
                     /old_locus_tag="pgaptmp_002469"
     CDS             9447..10346
                     /gene="ybiY"
                     /locus_tag="ACHOSC_RS12345"
                     /old_locus_tag="pgaptmp_002469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415345.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycyl-radical enzyme activating protein"
                     /protein_id="HGU4548396.1"
                     /translation="MIFNIQRYSTHDGPGIRTVVFLKGCSLGCRWCQNPESRARTQDL
                     LYDARLCLEGCELCAKAAPEVIERALNGLLIHREKLTPEHLTALTDCCPTQALTVCGE
                     VKSVEEIMTTVLRDKPFYDRSGGGLTLSGGEPFMQPEMAMALLQASHEAGIHTAVETC
                     LHVPWKYIAPSLPYIDLFLADLKHVADAPFKQWTDGNAARVLDNLKKLAAAGKKIIIR
                     VPLIQGFNADETSVKAITDFAADELHVGEIHFLPYHTLGINKYHLLNLPYDAPEKPLD
                     APELLDFAQQYACQKGLTATLRG"
     gene            10352..12784
                     /gene="ybiW"
                     /locus_tag="ACHOSC_RS12350"
                     /old_locus_tag="pgaptmp_002470"
     CDS             10352..12784
                     /gene="ybiW"
                     /locus_tag="ACHOSC_RS12350"
                     /old_locus_tag="pgaptmp_002470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycyl radical protein"
                     /protein_id="HGU4548397.1"
                     /translation="MTTLKLDTLSDRIKAHKNALVHIVKPPVCTERAQHYTEMYQQHL
                     DKPIPVRRALALAHHLANRTIWIKHDELIIGNQASEVRAAPIFPEYTVSWIEKEIDDL
                     ADRPGAGFAVSEENKRVLHEVCPWWRGQTVQDRCYGMFTDEQKGLLATGIIKAEGNMT
                     SGDAHLAVNFPLLLEKGLDGLREKVAERRSRINLTVLEDLHGEQFLKAIDIVLVAVSE
                     HIERFAALAREMAATETRESRRDELLTMAENCDLIAHQPPQTFWQALQLCYFIQLILQ
                     IESNGHSVSFGRMDQYLYPYYRRDVELNQTLDREHAIEMLHSCWLKLLEVNKIRSGSH
                     SKASAGSPLYQNVTIGGQNLVDGQPMDAVNPLSYAILESCGRLRSTQPNLSVRYHAGM
                     SNDFLDACVQVIRCGFGMPAFNNDEIVIPEFIKLGIEPQDAYDYAAIGCIETAVGGKW
                     GYRCTGMSFINFARVMLAALEGGRDATSGKVFLPQEKALSAGNFNNFDEVMDAWDTQI
                     RYYTRKSIEIEYVVDTMLEENVHDILCSALVDDCIERAKSIKQGGAKYDWVSGLQVGI
                     ANLGNSLAAVKKLVFEQGAIGQQQLAAALADDFDGLTHEQLRQRLINGAPKYGNDDDT
                     VDTLLARAYQTYIDELKQYHNPRYGRGPVGGNYYAGTSSISANVPFGAQTMATPDGRK
                     AHTPLAEGASPASGTDHLGPTAVIGSVGKLPTAAILGGVLLNQKLNPATLENESDKQK
                     LMILLRTFFEVHKGWHIQYNIVSRETLLEAKKHPDQYRDLVVRVAGYSAFFTALSPDA
                     QDDIIARTEHML"
     gene            12930..13745
                     /gene="ybiV"
                     /locus_tag="ACHOSC_RS12355"
                     /old_locus_tag="pgaptmp_002471"
     CDS             12930..13745
                     /gene="ybiV"
                     /locus_tag="ACHOSC_RS12355"
                     /old_locus_tag="pgaptmp_002471"
                     /EC_number="3.1.3.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar-phosphatase YbiV"
                     /protein_id="HGU4548398.1"
                     /translation="MSVKVIVTDMDGTFLNDAKTYNQPRFMAQYQELKKRGIEFVVAS
                     GNQYYQLISFFPELKDEISFVAENGALVYEHGKQLFHGELTRHESRIVIGELLKDKQL
                     NFVACGLQSAYVSENAPEAFVALMAKHYHRLKAVKDYQEIDDVLFKFSLNLPDEQIPL
                     VIDKLHIALDGIMKPVTSGFGFIDLIIPGLHKANGISRLLKRWDLSPQNVVAIGDSGN
                     DAEMLKMARYSFAMGNAAENIKQIARYATDDNNHEGALNVIQAVLDNTSPFNS"
     gene            13897..15162
                     /gene="ybiU"
                     /locus_tag="ACHOSC_RS12360"
                     /old_locus_tag="pgaptmp_002472"
     CDS             13897..15162
                     /gene="ybiU"
                     /locus_tag="ACHOSC_RS12360"
                     /old_locus_tag="pgaptmp_002472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1479 domain-containing protein"
                     /protein_id="HGU4548399.1"
                     /translation="MASTFTSDTLPADHKAAIRQMKHALRAQLGDVQQIFNQLSDDIA
                     TRVAEINALKAQGDAVWPVLSYADIKAGHVTAEQREQIKRRGCAVIKGHFPREQALGW
                     DQSMLDYLDRNRFDEVYKGPGDNFFGTLSASRPEIYPIYWSQAQMQARQSEEMANAQS
                     FLNRLWTFESDGKQWFNPDVSVIYPDRIRRRPPGTTSKGLGAHTDSGALERWLLPAYQ
                     RVFANVFNGNLAQYDPWHAAHRTEVEEYTVDNTTKCSVFRTFQGWTALSDMLPGQGLL
                     HVVPIPEAMAYVLLRPLLDDVPEDELCGVAPGRVLPVSEQWHPLLIEALTSIPKLEAG
                     DSVWWHCDVIHSVAPVENQQGWGNVMYIPAAPMCEKNLAYAHKVKAALEKGASPGDFP
                     REDYETNWEGRFTLADLNIHGKRALGMDV"
BASE COUNT         3691 a         4237 c         3911 g         3466 t
ORIGIN      
        1 actatctgaa cgctgtgccg ctgcaaaaat agtcccttgc tgcggctttt taaccaaatt
       61 acccgattct acaaaatcag cagaaagtta caacacttgc ttagggcaat aatttcctgt
      121 gaggtgatta ccctttcaag caatattcaa acgtaactat cctttaattt tcggatccag
      181 cgcatcgcgt aaaccatcgc ccaacaaatt gaacgccagt acggtcagaa aaatagccag
      241 ggccggaaaa acagcgacat gcggcgcgat aaccatatcc gctcgtgcct cattgagcat
      301 tgctccccac tctggtgtcg gcggctgcgc accgaggccg agaaatgaca ggctggcggc
      361 agagataatc gaggtgccaa tgcgcatggt gaaaaacacc acgatagacg agacggtccc
      421 aggcaagata tgacgcaata aaatggtcat atcgctggca ccaatactgc gtgctgactc
      481 aataaaggtt tgctgtttca acaccagcgt gttgccgcgc accagtcggg caaacgcggg
      541 gatggaaaaa atggcgactg cgataatcac gttagcaatg ccgcttccca acaccgcaac
      601 aacagcgatc gccagtaaaa tacccgggaa ggcaaacagc acatcgcaaa tgcgcatgat
      661 caaccgatcc caccagcctt cataatatcc agccaacaag cccagcaacg tcccgatcgc
      721 cgcaccgata aacacggcaa acacgcccgc cgccagcgag atttgcgcac caaccaggac
      781 acggctgaaa atgtcgcgcc ccagtgaatc gacgccaaac cagtgctgca aagaaggtcc
      841 gttattcaga ttgtcataat caaaataatt ttcggcgtca tagggagcga tccagcgtgc
      901 aaaaatggcc accacaatca ataaaataac gaataatgcg gcggtcatcg ccatatgctg
      961 acggcgaaat cgtcgccaga attcatgcca cggtgtacgc acctggtcag gtttgaccag
     1021 tggcatggcg tttaacaccg cctgacgtcg ccagttaaat agtcgcatcc ttacttgtac
     1081 ctgatagccg ggttaatggc ggcgtaaagc acatccacca ctaagttgat aagaataaat
     1141 tccagcgaga aaagcagaat ttccgcctga atcaccgggt aatcgcgcat ttctacggag
     1201 tcaacgagta agcgtccaag tccgggccag ttgaaaactt tctccaccac gatagaacca
     1261 ccgagcaaaa agccaaactg taagcccatc atggtcacta ccgggatcat cgcgttacgt
     1321 agcccgtgtt tgaggacaac ccaggtttcg ctcacccctt tcgccctcgc ggtacgcata
     1381 taatcttcgc ttaaaacatc gacaaacgac gcgcgggtaa agcgcgccat cacggctgcc
     1441 actgccgcgc cgagcgtcag ggagggtaaa atgtagtgct gccaactgtc tgctcccacg
     1501 gtaggcagcc agcccagttc aacggagaat acctgaatta aaagcatccc cagcgcaaat
     1561 gccggaaacg agatccccga caccgcaatg gtcatactca atcgatccgg ccaacgatta
     1621 cgccagacgg cggcgataat tcccgccgcc ataccaaata tcaccgccca gaccatactg
     1681 gttatggtca gccacagcgt tggcataaag cggctggcaa tctcatcggc aaccggacga
     1741 cgcgacacca tcgacaggcc aaaatccccc tgcacagcat tgctgatata gtgccagaac
     1801 tggtgataca gcggctgatc caaccccagc tgctgacgca ccagttctat aacctgcgca
     1861 tcagcttcgg gcccggcaat caatcgcgcc ggatcgccgg gcagcatatg cacaaataaa
     1921 aacaccagca ccgagacgat aaacagcgtc ggaatcaacc ccagtaagcg tttgataacg
     1981 taattaagca ttccactccc tgcgttgctt attgcaaatc cgcgtcttca aagctgaagc
     2041 cggtgtctgg catgatccaa aaaccggtca ggtttttact gtgtgccgac accagttttt
     2101 ctaccaccag cgggatccac ggcgactctt gccagatgat atcctgcgcc gccttataca
     2161 agcgggtctt ttccgccgga tcattagttt tcagtgcctg agccaggaag tcgtccacct
     2221 gtttattgct gtaaaatgcg gtattaaaca gcgtcggtgg ccagttttgc gaggcgaaca
     2281 gtggcgatag cgcccagtcc gcttcgccgg ttgaagccga ccagccagtg tagaacatcc
     2341 gcacgccgct ctctttttgc cctttacctt caacttctgc cgcccgctgt ccggcatcca
     2401 tcgcggtcac ctgggcttta atcccgacct gcgctaactg ctgctgggta aattgcagca
     2461 ctttctgcgc ggtgctgtgg ttatgtgacg accacagcgt ggtactgaaa ccgttgggat
     2521 atcccgcctc tttcagtaat tcgcgcgctt tcactggatc gtaaggccac ggtttataac
     2581 tttgcgcgta ggcgatactt ggcggtacca caccagtagc tggcgttgca tagcccgcaa
     2641 aggcaacttt caccagcgcc ggacggttaa tggcgtaatt cagcgcctca cggaccttcg
     2701 ggttatcgaa cggcttttgc gtcacgttca tactgatata acgctgcata attgacggac
     2761 tggccatcaa ctcgatattt ttgtttttct ccagcagtgt ggcctgctcg tagggaatgg
     2821 ggaaagcaaa ctgcgcttca ccggtttgca gcattgccgc gcgggtgttg ttatccgcca
     2881 ccggacgcca ggttatgctg tccagtttgg gcaatcctgg ctgccagtaa cccgcgaatt
     2941 ttttcacctt cacaaaatcg gtctgattcc aggtatccag ttcatacggt ccggttccca
     3001 ccggatgaaa accaatctcc ttgccatatt tttccagcgc tgccggtgaa atcatcgcgg
     3061 tcgccggatg ggcaagaata ttaataaacg ctgagaacgg ctgtttgagg gtaatcttta
     3121 ccgttgtcgg atcgatcgcc tccgttttgg caatattctt atacaggtta tagcgtttaa
     3181 gatgattcgc cggatcgctg gcccggtcca gattcgcttt taccgccact gcgttgaaat
     3241 cggtgccatc ctggaattta attccttccc gcaatttcac gatgtaagta atgccgtcat
     3301 cggaaacggt ataactctcc gccagcacgt ttttcagttt catctcttta tccagaccga
     3361 acagcccctg gtaaaacgat ttcgctacgg cctgagataa cgtgtcattt gcgtcatacg
     3421 gatcgagcgt ggtgaaattc gatcctaccg ccaccaccac atctttggca gcgaatgcac
     3481 aagatgccat caacgctgtc gcaatgccca gcgccactaa cccactacgg tgtacagctc
     3541 ttgccatttt attctccgcc tgaatgttat ctacgcatga atgcgtattc tgattgtggt
     3601 tgtgcgacgt aatgccccgg cccgacgcat tgcaacgaga cggctgccac ctcttcgcca
     3661 cgcagatgaa tattgctggg aagatcgtcc gacagcagta cacgctgcgg tcgttgtcgg
     3721 gacggttcag cgaccggaac tgccgccagt aatttacgcg tataaggatg ctgcgggttt
     3781 tcgaagaccg cgcgccgtgg accaatttca acaatttgcc cgagatacat caccgccaca
     3841 cgatgactaa tccgctctac cacggccata tcgtgggaga taaacagata cgcaatgccg
     3901 aaatcacgct ggagatcgag caacaagttg ataatctgcc cgcgaataga aacatccagc
     3961 gccgaaacgg cttcgtcggc aatgatcact tttggattca atgccaacgc gcgagcaatg
     4021 cagatgcgct ggcgctgacc gccggaaaac tcatgcgggt aacgccaggc atgttcaggt
     4081 aacaggccca cgcgctccag caaccacgca acgcgtgcag ccgcgtcttt acctggcaat
     4141 aaaccgtgta cacgcagcgg ttcgataatc gaatcaccga tggtctgacg tgggtccagc
     4201 gaagcgtaag ggtcctgaaa aataaactga atatcccggc gcaatgcctg aagtttgccg
     4261 ggtgacaagg tatcgattct ctgaccgtta aagataattt cgccgccctg cgattcgacc
     4321 aggcgcagca acgcccgccc ggtagtggat ttaccgctgc cagactcgcc caccagcgat
     4381 agcgtttcgc caggccagag atcaaaacta actttctcaa cggcatgcac ttcccgcgtt
     4441 acgcgattca acaaaccgct gcgcaaaggg aaacgggtga caagattacg cactcgtaaa
     4501 acaggttcgc catccaccac cgttttctgc tcgatggggg gggcctgttt cgctggatgt
     4561 tcaagcgata tcaacgggaa acgtcgggga taatctaacc ctttcatcgc accaagttgc
     4621 ggaacagcag ctaacagcgc acgggtgtaa ggatgttgcg gtgcatgaaa aatctgttcg
     4681 acggtacccg tttccaccgc ctcgccctga tacatcacca gtacccgatc ggcaatctct
     4741 gccaccacgc ccatatcgtg agtgataaag ataacgccca tcgacatctc tttttgcaat
     4801 actttgatta attgcaggat ctgcgcctga atagtgacat ccagcgcggt ggttggctca
     4861 tcggcaatca gcaccgccgg gcggcatgac agcgccatcg caatcatcac tcgctggcgc
     4921 atcccgccag agagttgatg cggataacgt gaaagaatgg tttgtgcctc aggaatgcgt
     4981 acctgatcca gcatccgctt cgcctcgacc atcgcttctt cacgactggc gttctgatgc
     5041 agacgaattg attcggcaat ctgttcaccc acagtaaata ccgggttcag cgatgtcatc
     5101 ggctcctgaa atatcatcgc catatccgca ccgcgaacat ggcgcatttg tgcagcgctc
     5161 tgctcgctaa gttcaatcac ttcgcgactg cgccgctgca acagcatttt atcgcactgt
     5221 actaaaccgc ccgcctgttc caacaggcgc atcaatgcca acgcagtcac tgacttaccg
     5281 gagccggatt cgccaacaat tgccagcgtc tcaccgcgtt gcagactaaa agagagattg
     5341 cggaccgcag ctattttctg ctggtcctgc ataaaggcaa tattcagatt ttcaaccgcc
     5401 agcacattac cggcatcaag ttcatcactg tgtggcaacg gtgtcccctt tttcacggta
     5461 gataccggtg gttggcgtat cgcctgcgta gccccaggcg cgatacattc cttcggtgtt
     5521 aaacggtagc gcgacattcc cttcatggtc gatagcgatt aagccaccgc taccgccaag
     5581 cgcagggagt ttttccatta ctacccgctc gcaggcttcc gcgagactta atccgccgta
     5641 atccattaac gcggcgatgt catatgccgc cagcgcgcgg atgaagactt cgcccgtgcc
     5701 ggtacaagaa accgccacac tggcgttatt ggcgtagcat ccggcaccca ctaagggact
     5761 atcgccaact cgtccgggta atttattggt cattccgcct gtggacgtgg ctgccgccaa
     5821 attgccgtct aaatccaacg ccacggcccc cacggtgccc attttctgtt tttcatccag
     5881 tggcgcaccg ctatggtcga ggacggttgc cccttccttg cgcgctgcca gtagttgttc
     5941 ataacgcaaa ggcgtggaga aaatctccgg cgagacgcgc tccatgccac gagcaaacgc
     6001 aaaattttct gccccttcgc caatcatcat cacatgcggg ctttgctcca tcaccagccg
     6061 ggcggcaaga accggattac gcagatgact aacgcccgcc accgcaccgg ctttcagggt
     6121 gttaccatcc atcacacagg cgtccagttc atgggtttca tcacgcgtaa agacagcgcc
     6181 aattccggcg ttaaacagtg gacactcttc cagcagacgc accgcttccg tcaccacatc
     6241 cagcgcactt tcgcccgctt ccaacatttt ctgcccggtt tcaacaatgg cagacaacgc
     6301 ctcaatgtag cgtaattcct gttgcagact catctgcgcg cggctaattg cacctgcgcc
     6361 accatgaatt gcaatgactg ctttgcccat agtattcgtc cattaagggt ggctcgccac
     6421 ttttgctata aatatcagaa tttttatgaa ttactcattc aatttttgaa tatagaaaga
     6481 tggatatggc atgtaaaggc aggagccaac gctacgcccg aggtggcata cagtctgtgg
     6541 cgtttttctg acataatagg caaattcgat tttgcctccg caggagtgtt ttcatggaat
     6601 ttaccaccgg attgatgtcg ctcgacaccg cgcttaatga gatgctttct cgcgtcaccc
     6661 cactgaccgc ccaggaaacg ctgccactgg tacagtgttt tggtcgtatt ctggcgagcg
     6721 atgtcgtttc gccgcttgat gttccggggt ttgataactc cgcaatggac ggctacgcgg
     6781 tgcgtttagc cgatattgcc tccaggcaac cgctgcccgt tgccggtaaa tcctttgccg
     6841 gtcagccgta tcatggtgaa tggcctgcgg gcacctgcat tcgtattatg accggtgcgc
     6901 cggtgccgga aggctgcgaa gcggtggtga tgcaggagca gactgaacaa acggacaatg
     6961 gcgtgcgttt tactgctgaa gtgcgtagcg ggcaaaatat tcgccgtcgc ggtgaagata
     7021 tctctgcagg tgcggttgtt ttcccggcgg gaactcgcct gactaccgca gagctgccag
     7081 taattgcttc actggggatt gccgaagttc cggtgattcg taaagtgcgt gtagcgcttt
     7141 tttctaccgg tgatgaactc cagttgcccg gtcagccgct gggcgacggc caaatctacg
     7201 acaccaaccg tctcgccgta cacctgatgt tagaacagct gggttgcgag gtaatcaact
     7261 tagggattat ccgcgacgat ccccatgccc tgcgcgccgc atttattgaa gccgacagcc
     7321 aggcggatgt agtgatcagt tccggcggtg tttcagtggg tgaagcggat tacaccaaaa
     7381 cgattcttga agagctgggg gagatcgcct tctggaagct ggcgattaaa ccaggtaaac
     7441 cgttcgcgtt cggtaaactc agcaatagct ggttctgcgg cctgccgggc aacccggttt
     7501 cagcgacgct gaccttctat caactggtac agcctttgct ggcaaaacta agcggcaaca
     7561 ccgccagcgg cctgcccgcg cgccagcgcg tacgcacagc gtcccgcctg aaaaaaacgc
     7621 caggacgtct tgatttccag cgcggcgtgc tgcaacgcaa cgccgacggt gaacttgaag
     7681 tgacgaccac cggacatcag ggttcacata tatttagctc ctttagtctc ggcaactgct
     7741 ttatcgtgct ggaacgcgat cgcggcaatg tggaagtggg cgaatgggtg gaagtagaac
     7801 cgtttaacgc gttgttcgga ggcctgtaat gacggaactc agcgatcagg agatgctgcg
     7861 ctacaaccgg caaatcattc tgcgcggttt tgattttgac ggtcaggagg cgctaaaaga
     7921 ttctcgtgtg ctggtagtcg gcctcggtgg actcggctgc gcagcttcgc agtatctggc
     7981 aagcgccggt gtcggcaacc tgacgctgct cgacttcgac tcggtttcgc tctcgaatct
     8041 gcaacgccag acgctgcaca gtgatgctac ggtcgggcaa ccgaaggtag aatccgcccg
     8101 tgacgccatg acgcggatca acccacatat cgcgattacg ccagtcaatg cactgcttga
     8161 tgacgctgaa cttgctgcga tgattgcaaa acacgatctg gtactcgact gtacggataa
     8221 cgttgcggtg cgtaatcaac tgaacgctgg ctgttttgcc gcgaaggtgc cgctggtttc
     8281 cggcgcggca attcgtatgg aagggcaaat cacggtcttc acttatcagg acggcgaacc
     8341 gtgctatcgc tgccttagcc gtttgtttgg cgagaacgca ttaacctgcg tagaagcagg
     8401 cgtgatggca ccgttgatcg gcgtaattgg ttcgttgcag gcaatggaag cgatcaaact
     8461 gctggcaggt tatggcaaac cggccagcgg gaaaatcgtc atgtacgatg caatgacctg
     8521 tcagtttcgc gaaatgaaac tgatgcgcaa tccggggtgt gaggtgtgcg ggcagtaatt
     8581 gccacctcca ggccagataa gacgcggcac tccgccacgt catcagcaat ttatgaggat
     8641 gcagaacgtc cggttaaatc gacgttctgc caaacgctcc cagccagtcc tgttcaaact
     8701 tcgccacagc ggcttccacc gccggatagc taatcatctg ttgtgccaca tccagtggca
     8761 gcgtaattga ttcacatcct gccagtaagc agtccagcgc ctgacgcggg gttttgaaac
     8821 tcgctgccag cactttcgcc tgcggcgcat gcattttcaa taactggtgt aagtcggtca
     8881 cagtctgaat gccgctaccg ccctgagcat caatacgatt aacgtaaggc gcaacatatt
     8941 ccgcacctgc cagcgccgac agcagccctt gtgctgcgcc atataccgcg gttcccagcg
     9001 ttggaatccc ttccgctttt aacatcttaa tagctgccat cccctcggcg gtcaccggaa
     9061 ctttcaccac gatatccgca ataatagaac gcaatttacg tgcgtcattc accatccctt
     9121 cagcagtggt agccattacc tgggcaaaca gacgcccctg accgcccatc gcttcatgaa
     9181 gttgcggaag cacaacatcc agcggtttgt tacccgcggc gataatgctt gggttagtgg
     9241 tcacacccgc cagcggaaaa atacgtgaca gcgccttcac cgcaacaacg tctgaagtat
     9301 ccagatacag ttccataacc atcctcaaaa tgtacttttt gtctgaatgt agactatcac
     9361 gggaaaactc tctgaacagt tgacgcacat caatctaact ttcattcgaa agcattttaa
     9421 tgtttgaacg aaacagagag gcaactatga ttttcaatat tcagcgctac tcgacccatg
     9481 atggccccgg tatccgcacg gtcgtatttc ttaaaggctg ttcgctgggc tgccgctggt
     9541 gtcagaaccc ggaaagccgc gcccgcacgc aggatctgct gtatgacgca cgactgtgtc
     9601 tggaaggctg cgagctgtgc gctaaggccg cgccggaagt gattgagcgc gcgctgaatg
     9661 gtttgcttat tcatcgggaa aagttaaccc cggagcatct gacggcgtta accgactgct
     9721 gtccgacaca ggcattaacc gtgtgtggtg aagtgaaaag cgttgaggag atcatgacga
     9781 ccgttctgcg cgataaaccg ttttatgatc gcagcggcgg cggtttaacg ctttcgggtg
     9841 gtgagccctt tatgcagccg gaaatggcga tggcgctact gcaagccagc cacgaggcag
     9901 gcattcatac tgcggtagaa acctgtctgc atgtgccgtg gaaatatatc gccccttctc
     9961 tgccctatat cgatctgttt cttgccgatt taaaacacgt tgccgacgcg ccgtttaaac
    10021 agtggaccga cggtaacgcc gccagagtgc tggataacct gaaaaaactc gccgcagcgg
    10081 gcaaaaaaat cattatccgc gtgccgctga ttcagggctt taatgccgac gaaacctctg
    10141 taaaagccat taccgatttt gccgccgacg agctgcacgt tggcgaaatt cattttctgc
    10201 cctaccacac gctgggcatc aacaaatatc acttacttaa tctgccctat gacgccccgg
    10261 aaaaaccgct tgatgcgcca gaactgctcg actttgccca gcagtatgcc tgccagaaag
    10321 ggttaaccgc gaccttacga ggataacaac catgaccaca ctgaaactgg acacgctcag
    10381 cgaccgcatt aaagcgcaca aaaatgcgct ggtgcatatt gtgaaaccgc cagtctgtac
    10441 cgagcgcgcg cagcactata ccgagatgta tcaacaacat ctcgataagc cgatcccggt
    10501 acgtcgcgcg ctggcactgg cgcatcacct ggcgaatcgc accatctgga tcaaacacga
    10561 tgagttgatc attggcaacc aggcaagcga agttcgcgcc gcgccgatct tcccggaata
    10621 tactgtctcg tggatcgaaa aagagattga tgatctggca gatcgtcccg gtgctggctt
    10681 tgcggtgagc gaagagaaca aacgcgttct gcatgaagtg tgcccgtggt ggcgcggtca
    10741 gaccgtacag gatcgctgct acggcatgtt taccgatgag caaaaaggtc tgctggcgac
    10801 cggaatcatt aaagcggaag gcaatatgac ctccggcgat gcgcacctgg cggtgaattt
    10861 cccgctgctg ctggaaaaag gtcttgatgg tctgcgtgag aaagtagcgg aacgtcgctc
    10921 gcgcatcaat ctgacggtgc tggaagattt acatggcgaa caattcctga aagcgattga
    10981 tatcgtgctg gtggcagtca gtgaacacat tgaacgtttc gctgccctgg cgcgtgaaat
    11041 ggctgctacc gaaactcgcg aaagccgtcg cgatgaactg ctgacgatgg cagaaaactg
    11101 cgatcttatc gcccaccagc cgccgcagac tttctggcag gcgctgcaac tgtgttactt
    11161 catccaattg attttgcaga tcgaatctaa cggtcactcg gtatcgtttg gtcgtatgga
    11221 ccagtatctc tacccgtact atcgccgcga cgttgaactc aaccagacgc tggatcgcga
    11281 acacgccatc gagatgctgc atagctgctg gctgaaactg ctggaagtga acaagatccg
    11341 ctccggctca cactcaaaag cctctgcggg aagtccgctg tatcagaacg ttaccattgg
    11401 cggacaaaat ctggttgatg gtcaaccaat ggatgcagtg aatcccctct cttacgcgat
    11461 cctcgaatcc tgcggtcgcc tgcgttccac tcagcctaac ctcagcgtgc gttaccatgc
    11521 aggaatgagt aatgatttcc tcgacgcctg cgtacaggtg atccgttgcg gcttcgggat
    11581 gccggcgttc aacaacgacg aaatcgtgat cccggaattt attaaactcg gtattgaacc
    11641 gcaggacgct tacgactacg cagcgattgg ttgtatcgaa accgccgtcg gtggcaaatg
    11701 gggctatcgc tgtaccggca tgagctttat caacttcgcc cgcgtgatgc tggcggcgct
    11761 ggaaggcggg cgtgatgcca ccagcggcaa agtgttcctg ccacaagaaa aagcgttgtc
    11821 ggcaggcaac tttaacaatt tcgatgaagt aatggacgcg tgggatacgc aaatccgtta
    11881 ttacacgcgt aaatcaatcg aaatcgaata tgtcgtcgac accatgctgg aagagaacgt
    11941 gcacgatatt ctctgctcgg cgctggtgga tgactgtatt gagcgagcga aaagtatcaa
    12001 gcaaggcggc gcgaaatatg actgggtttc tggcctgcag gttggcattg ccaacctcgg
    12061 caacagcctg gcggcagtga agaaactggt atttgaacag ggcgcgattg gtcagcaaca
    12121 gctggctgcc gcactggcgg atgacttcga tggcctgact catgagcaac tgcgtcagcg
    12181 gctgattaac ggtgcgccga agtacggcaa cgacgatgat actgtcgata cgctgctggc
    12241 tcgcgcttat cagacctata tcgacgaact gaaacagtac cataatccgc gctacggtcg
    12301 tggtccggtt ggcggcaact attacgcggg tacgtcatct atctccgcta acgtaccgtt
    12361 tggcgcgcaa actatggcaa cgccggacgg gcgtaaagcc cacaccccgc tggcagaagg
    12421 cgctagtccg gcctctggta ctgaccatct cggccctact gcggtcattg gctcggtggg
    12481 taaactgcct acggcagcga ttctcggcgg cgtgttgctc aaccagaaac tgaatccggc
    12541 aacgctggag aacgaatctg acaagcagaa gctgatgatc ctgctgcgca ccttcttcga
    12601 ggtgcataaa ggctggcata ttcagtacaa catcgtttcc cgcgaaacgc tgctggaggc
    12661 gaaaaaacat ccggatcagt accgcgatct ggtagtgcgt gtcgcgggtt attccgcctt
    12721 cttcaccgcg ctctctccag acgctcagga cgatatcatc gctcgtactg aacatatgct
    12781 gtaatctctt cggcccggct gcggcgtcgc cgggctaaat cattcacatc atcaatttca
    12841 tccttacttt cattcgaaat ataatttgtg ctctgcgtca cattgttatt acactgttac
    12901 ggtttcattt tcgagccagg agcacaccta tgagcgtaaa agttatcgtc acagacatgg
    12961 acggtacttt tcttaacgac gccaaaacgt acaaccaacc acgttttatg gcgcaatatc
    13021 aggaactgaa aaagcgcggc attgagttcg ttgtcgccag cggtaatcag tattaccagc
    13081 ttatttcgtt ctttcctgag ctaaaggatg agatctcttt tgtcgcggaa aacggcgcac
    13141 tggtttacga acatggcaag caactgttcc acggcgaact gacccgacat gaatcgcgga
    13201 ttgttattgg cgagttgcta aaagataagc aactcaattt tgtcgcctgc ggtctgcaaa
    13261 gtgcatatgt cagcgaaaat gcccccgaag catttgtcgc actgatggca aaacactacc
    13321 atcgcctgaa agctgtaaaa gattatcagg agattgacga cgtactgttc aagttttcgc
    13381 tcaacctgcc agatgaacaa atcccgttag tgatcgacaa actgcacata gcgctcgatg
    13441 gcattatgaa acccgtcacc agtggttttg gctttatcga cctgattatt cccggtctac
    13501 ataaagcaaa cggtatttcg cggttactga aacgctggga tctgtcaccg caaaatgtgg
    13561 tagcgattgg cgacagcggt aacgatgcgg agatgctgaa aatggcgcgt tattcctttg
    13621 cgatgggcaa tgctgcggaa aacattaaac aaatcgcccg ttacgctacc gatgataata
    13681 atcatgaagg cgcgctgaat gtgattcagg cggtgctgga taacacatcc ccttttaaca
    13741 gctgacctct ctaccggagc cgtgcgctcc ggttttccct tctttttaca tcctgtacat
    13801 cacatcaaat cattaaactt gcattaactt atttataact tatattgtca catgcaaatc
    13861 agaatggttt cgaatgaaag ttaatgagag gtggttatgg cttctacttt taccagcgac
    13921 acattgcctg ccgatcacaa agcagctatc cgtcagatga agcacgcgct gcgggcgcag
    13981 cttggcgacg tccagcagat ctttaatcag ctaagcgatg acattgccac gcgagtggct
    14041 gaaatcaacg cactcaaagc acagggcgat gccgtctggc cggtgctgtc ttatgccgat
    14101 atcaaagcag gtcatgttac tgcagagcag cgcgaacaga ttaaacgtcg cggttgtgcg
    14161 gtgataaaag gccatttccc ccgcgaacaa gcgctaggct gggatcagtc gatgctggac
    14221 tatctggacc gcaaccgctt tgacgaggtc tacaaaggcc ccggcgataa tttcttcggg
    14281 acgctcagcg cttcacgtcc cgagatttac cccatctact ggtcgcaggc gcaaatgcag
    14341 gcccgccaga gtgaagaaat ggcgaatgcg cagtcgtttc tcaatcgtct gtggacattt
    14401 gaaagtgatg gaaagcaatg gtttaacccg gatgtgagcg tcatctaccc tgaccgtatc
    14461 cgccgccgtc cgcccggaac gacctccaaa ggtcttggag cgcataccga ctccggggcg
    14521 ctggaacgct ggctgcttcc agcgtatcag cgcgttttcg ccaacgtctt taatggcaat
    14581 ctggcgcaat atgatccctg gcatgcggca catcgtacgg aagttgaaga gtacacggtg
    14641 gacaacacca ccaaatgttc cgtgtttcgg acattccagg gctggacagc gctctctgat
    14701 atgctgcctg gtcaggggct gctgcacgtt gtgcccattc ctgaagccat ggcgtacgta
    14761 ctgttacgtc cgctgcttga tgatgtgccg gaggatgaac tgtgcggcgt agcgcccgga
    14821 agagtgttgc cggtatcaga gcaatggcat ccactgttaa ttgaggcgtt aaccagcatt
    14881 ccaaaactcg aggccggaga ctccgtctgg tggcactgcg acgtcatcca ttccgttgcc
    14941 cccgttgaaa atcaacaggg ttggggcaac gtgatgtaca ttcctgcggc accgatgtgc
    15001 gagaaaaatc ttgcctacgc gcacaaggtg aaggccgcac tggaaaaagg cgcatcgccg
    15061 ggcgacttcc cgcgcgagga ctatgaaaca aactgggaag gacgctttac gcttgccgac
    15121 ctcaacattc acggtaagcg agcgttgggc atggatgttt gagagttaat ggaagtgaat
    15181 tgatgtggga ttaagaaaat cgattcgacg ttactcagat tgctgacaag tgcgctttct
    15241 taatgcctga tgcgctacgc ttatctggcc tacaaagtcc tgcagattca atatattaca
    15301 ggtac
//
LOCUS       DAZKHF020000097        15251 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2201S31241
            SAMN43267800-rid25106413.denovo.098, whole genome shotgun sequence.
ACCESSION   DAZKHF020000097 DAZKHF020000000
VERSION     DAZKHF020000097.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267800
            Sequence Read Archive: SRR30307263
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 15251)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15251)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 15251)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350581.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350581.2
            Genome Coverage       :: 151x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:21:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,652
            CDSs (total)                      :: 5,557
            Genes (coding)                    :: 5,320
            CDSs (with protein)               :: 5,320
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 237
            CDSs (without protein)            :: 237
            Pseudo Genes (ambiguous residues) :: 0 of 237
            Pseudo Genes (frameshifted)       :: 102 of 237
            Pseudo Genes (incomplete)         :: 130 of 237
            Pseudo Genes (internal stop)      :: 52 of 237
            Pseudo Genes (multiple problems)  :: 41 of 237
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15251
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267800-rid25106413.denovo.098"
                     /serotype="O 26:H11"
                     /isolate="2201S31241"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-01"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            81..647
                     /gene="yceJ"
                     /locus_tag="ACHOSC_RS12365"
                     /old_locus_tag="pgaptmp_002473"
     CDS             81..647
                     /gene="yceJ"
                     /locus_tag="ACHOSC_RS12365"
                     /old_locus_tag="pgaptmp_002473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0022904 - respiratory electron transport
                     chain [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome b"
                     /protein_id="HGU4548400.1"
                     /translation="MSFTNTPERYGVISAAFHWLSAIIVYGMFALGLWMVTLSYYDGW
                     YHKAPELHKSIGILLMMGLVIRVLWRVISPPPGPLPSYSPMTRLAAKAGHLALYLLLF
                     AIGISGYLISTADGKPISVFGWFDVPATLADAGAQADFAGALHFWLAWSVVVLSVMHG
                     FMALKHHFIDKDDTLKRMLGKSSSDYGV"
     gene            651..1227
                     /gene="yceI"
                     /locus_tag="ACHOSC_RS12370"
                     /old_locus_tag="pgaptmp_002474"
                     /pseudo
     CDS             651..1227
                     /gene="yceI"
                     /locus_tag="ACHOSC_RS12370"
                     /old_locus_tag="pgaptmp_002474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309461.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="YceI family protein"
     gene            complement(1269..2321)
                     /gene="trhO"
                     /locus_tag="ACHOSC_RS12375"
                     /old_locus_tag="pgaptmp_002475"
     CDS             complement(1269..2321)
                     /gene="trhO"
                     /locus_tag="ACHOSC_RS12375"
                     /old_locus_tag="pgaptmp_002475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706977.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-related sulfurtransferase"
                     /protein_id="HGU4548401.1"
                     /translation="MPVLHNRISNDALKAKMLAESEPRTTISFYKYFHIADPKATRDA
                     LYQLFTALNVFGRVYLAHEGINAQISVPASNVETFRAQLYAFDPALEGLRLNIALDDD
                     GKSFWVLRMKVRDRIVADGIDDPHFDASNVGEYLQAAEVNAMLDDPDALFIDMRNHYE
                     YEVGHFENALEIPADTFREQLPKAVEMMQAHKDKKIVMYCTGGIRCEKASAWMKHNGF
                     NKVWHIEGGIIEYARKAREQGLPVRFIGKNFVFDERMGERISDEIIAHCHQCGAPCDS
                     HTNCKNDGCHLLFIQCPVCAEKYKGCCSEICCEESALPPEEQRRRRAGRENGNKIFNK
                     SRGRLNTTLGIPDPTE"
     gene            2546..3466
                     /gene="lpxL"
                     /locus_tag="ACHOSC_RS12380"
                     /old_locus_tag="pgaptmp_002476"
     CDS             2546..3466
                     /gene="lpxL"
                     /locus_tag="ACHOSC_RS12380"
                     /old_locus_tag="pgaptmp_002476"
                     /EC_number="2.3.1.241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="kdo(2)-lipid IV(A) lauroyltransferase"
                     /protein_id="HGU4548402.1"
                     /translation="MTNLPKFSTALLHPRYWLTWLGIGVLWLVVQLPYPVIYRLGCGL
                     GKLALRFMKRRAKIVHRNLELCFPEMSEQERRKMVVKNFESVGMGLMETGMAWFWPDR
                     RIARWTEVIGMEHIRDVQAQKRGILLVGIHFLTLELGARQFGMQEPGIGVYRPNDNPL
                     IDWLQTWGRLRSNKSMLDRKDLKGMIKALKKGEVVWYAPDHDYGPRSSVFVPLFAVEQ
                     AATTTGTWMLARMSGACLVPFVPRRKPDGKGYQLIMLPPECSPPLDDAETTAAWMNKV
                     VEKCIMMAPEQYMWLHRRFKTRPEGVPSRY"
     gene            3638..4864
                     /gene="mdtG"
                     /locus_tag="ACHOSC_RS12385"
                     /old_locus_tag="pgaptmp_002477"
     CDS             3638..4864
                     /gene="mdtG"
                     /locus_tag="ACHOSC_RS12385"
                     /old_locus_tag="pgaptmp_002477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter MdtG"
                     /protein_id="HGU4548403.1"
                     /translation="MSPCENDTPINWKRNLIVAWLGCFLTGAAFSLVMPFLPLYVEQL
                     GVTGHSALNMWSGIVFSITFLFSAIASPFWGGLADRKGRKLMLLRSALGMGIVMVLMG
                     LAQNIWQFLILRALLGLLGGFVPNANALIATQVPRNKSGWALGTLSTGGVSGALLGPM
                     AGGLLADSYGLRPVFFITASVLILCFFVTLFCIREKFQPVSKKEMLHMREVVTSLKNP
                     KLVLSLFVTTLIIQVATGSIAPILTLYVRELAGNVSNVAFISGMIASVPGVAALLSAP
                     RLGKLGDRIGPEKILITALIFSVLLLIPMSYVQTPLQLGILRFLLGAADGALLPAVQT
                     LLVYNSSNQIAGRIFSYNQSFRDIGNVTGPLMGAAISANYGFRAVFLVTAGVVLFNAV
                     YSWNSLRRRRIPQVSN"
     gene            4947..5321
                     /gene="msyB"
                     /locus_tag="ACHOSC_RS12390"
                     /old_locus_tag="pgaptmp_002478"
     CDS             4947..5321
                     /gene="msyB"
                     /locus_tag="ACHOSC_RS12390"
                     /old_locus_tag="pgaptmp_002478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309456.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MysB family protein"
                     /protein_id="HGU4548404.1"
                     /translation="MTMYATLEEAIDAAREEFLADNPGIDTEDANVQQFNAQKYVLQD
                     GDIMWQVEFFADEGEEGECLPMLSGEAAQSVFDGDYDEIEIRQEWQEENTLHEWDEGE
                     FQLEPPLDTEEGRAAADEWDER"
     gene            complement(5322..5549)
                     /gene="yceK"
                     /locus_tag="ACHOSC_RS12395"
                     /old_locus_tag="pgaptmp_002479"
     CDS             complement(5322..5549)
                     /gene="yceK"
                     /locus_tag="ACHOSC_RS12395"
                     /old_locus_tag="pgaptmp_002479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YceK/YidQ family lipoprotein"
                     /protein_id="HGU4548405.1"
                     /translation="MRLIVVSIMVTLLSGCGSIISRTIPGQGHGNQYYPGVQWDVRDS
                     AWRYVTILDLPFSLVFDTLLLPIDIHHGPYE"
     gene            complement(5722..8265)
                     /gene="mdoH"
                     /locus_tag="ACHOSC_RS12400"
                     /old_locus_tag="pgaptmp_002480"
     CDS             complement(5722..8265)
                     /gene="mdoH"
                     /locus_tag="ACHOSC_RS12400"
                     /old_locus_tag="pgaptmp_002480"
                     /EC_number="2.4.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016758 - hexosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucans biosynthesis glucosyltransferase MdoH"
                     /protein_id="HGU4548406.1"
                     /translation="MNKTTEYIDAMPIAASEKAALPKTDIRAVHQALDAEHRTWARED
                     DSPQGSVKARLEQAWPDSLADGQLIKDDEGRDQLKAMPEAKRSSMFPDPWRTNPVGRF
                     WDRLRGRDVTPRYLARLTKEEQESEQKWRTVGTIRRYILLILTLAQTVVATWYMKTIL
                     PYQGWALINPMDMVGQDLWVSFMQLLPYMLQTGILILFAVLFCWVSAGFWTALMGFLQ
                     LLIGRDKYSISASTVGDEPLNPEHRTALIMPICNEDVNRVFAGLRATWESVKATGNAK
                     HFDVYILSDSYNPDICVAEQKAWMELIAEVGGEGQIFYRRRRRRVKRKSGNIDDFCRR
                     WGSQYSYMVVLDADSVMTGDCLCGLVRLMEANPNAGIIQSSPKASGMDTLYARCQQFA
                     TRVYGPLFTAGLHFWQLGESHYWGHNAIIRVKPFIEHCALAPLPGEGSFAGSILSHDF
                     VEAALMRRAGWGVWIAYDLPGSYEELPPNLLDELKRDRRWCHGNLMNFRLFLVKGMHP
                     VHRAVFLTGVMSYLSAPLWFMFLALSTALQVVHALTEPQYFLQPRQLFPVWPQWRPEL
                     AIALFASTMVLLFLPKLLSILLIWCKGTKEYGGFWRVTLSLLLEVLFSVLLAPVRMLF
                     HTVFVVSAFLGWEVVWNSPQRDDDSTSWGEAFKRHGSQLLLGLVWAVGMAWLDLRFLF
                     WLAPIVFSLILSPFVSVISSRATVGLRTKRWKLFLIPEEYSPPQVLVDTDRFLEMNRQ
                     RSLDDGFMHAVFNPSFNALATAMATARHRASKVLEIARDRHVEQALNETPEKLNRDRR
                     LVLLSDPVTMARLHFRVWNSPERYSSWVSYYEGIKLNPLALRKPDAASQ"
     gene            complement(8258..9793)
                     /gene="mdoG"
                     /locus_tag="ACHOSC_RS12405"
                     /old_locus_tag="pgaptmp_002481"
     CDS             complement(8258..9793)
                     /gene="mdoG"
                     /locus_tag="ACHOSC_RS12405"
                     /old_locus_tag="pgaptmp_002481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucans biosynthesis protein MdoG"
                     /protein_id="HGU4548407.1"
                     /translation="MMKMRWLSAAVMLTLYTSSSWAFSIDDVAKQAQSLAGKGYEAPK
                     SNLPSVFRDMKYADYQQIQFNHDKAYWNNLKTPFKLEFYHQGMYFDTPVKINEVTATA
                     VKRIKYSPDYFTFGDVQHDKDTVKDLGFAGFKVLYPINSKDKNDEIVSMLGASYFRVI
                     GAGQVYGLSARGLAIDTALPSGEEFPRFKEFWIERPKPTDKRLTIYALLDSPRATGAY
                     KFVVMPGRDTVVDVQSKIYLRDKVGKLGVAPLTSMFLFGPNQPSPANNYRPELHDSNG
                     LSIHAGNGEWIWRPLNNPKHLAVSSFSMENPQGFGLLQRGRDFSRFEDLDDRYDLRPS
                     AWVTPKGEWGKGSVELVEIPTNDETNDNIVAYWTPDQLPEPGKEMNFKYTITFSRDED
                     KLHAPDNAWVQQTRRSTGDVKQSNLIRQPDGTIAFVVDFTGAEMKKLPEDTPVTAQTS
                     IGDNGEIVESTVRYNPVTKGWRLVMRVKVKDAKKTTEMRAALVNADQTLSETWSYQLP
                     ANE"
     gene            10187..11344
                     /gene="mdoC"
                     /locus_tag="ACHOSC_RS12410"
                     /old_locus_tag="pgaptmp_002482"
     CDS             10187..11344
                     /gene="mdoC"
                     /locus_tag="ACHOSC_RS12410"
                     /old_locus_tag="pgaptmp_002482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706958.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucans biosynthesis protein MdoC"
                     /protein_id="HGU4548408.1"
                     /translation="MNPVPAQREYFLDSIRAWLMLLGIPFHISLIYSSHTWHVNSAEP
                     SLWLTLFNDFIHSFRMQVFFVISGYFSYMLFLRYPLKKWWKVRVERVGIPMLTAIPLL
                     TLPQFIMLQYVKGKAESWPGLSLYDKYNTLAWELISHLWFLLVLVVMTTLCVWIFKRI
                     RNNLENSDKTNKKFSMVKLSVIFLCLGIGYAVIRRTIFIVYPPILSNGMFNFIVMQTL
                     FYLPFFILGALAFIFPHLKALFTTPSRGCTLAAALAFVAYLLNQRYGSGDAWMYETES
                     VITMVLGLWMVNVVFSFGHRLLNFQSARVTYFVNASLFIYLVHHPLTLFFGAYITPHI
                     TSNWLGFLCGLIFVVGIAIILYEIHLRIPLLKFLFSGKPVVKRENDKAPAR"
     gene            complement(11352..12833)
                     /gene="clsC"
                     /locus_tag="ACHOSC_RS12415"
                     /old_locus_tag="pgaptmp_002483"
     CDS             complement(11352..12833)
                     /gene="clsC"
                     /locus_tag="ACHOSC_RS12415"
                     /old_locus_tag="pgaptmp_002483"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706957.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cardiolipin synthase ClsC"
                     /protein_id="HGU4548409.1"
                     /translation="MMKKTPTSTKDSLPNKEMNDLPRLASAVLPLCSQHPGQCGLFPL
                     EKSLDAFAARYRLAEMAEHTLDVQYYIWQDDMSGRLLFSALLAAAKRGVRVRLLLDDN
                     NTPGLDDILRLLDSHPRIEVRLFNPFSFRLLRPLGYITDFSRLNRRMHNKSFTVDGVV
                     TLVGGRNIGDAYFGAGEEPLFSDLDVMAIGPVVEDVADDFARYWYCKSVSPLQQVLDV
                     PEGEMADRIELPASWHNDAMTHRYLRKMESSPFINHLVDGTLPLIWAKTRLLSDDPAK
                     GEGKAKRHSLLPQRLFDIMGSPSERIDIISSYFVPTRAGVAQLLRMVRKGVKIAILTN
                     SLAANDVAVVHAGYARWRKKLLRYGVELYELKPTREQSSTLHDRGITGNSGASLHAKT
                     FSIDGKTVFIGSFNFDPRSTLLNTEMGFVIESETLAQLIDKRFIQSQYDAAWQLRLDR
                     WGRINWVDRHAKKEIILKKEPATSFWKRVMVRLASILPVEWLL"
     gene            complement(12775..13308)
                     /gene="ymdB"
                     /locus_tag="ACHOSC_RS12420"
                     /old_locus_tag="pgaptmp_002484"
     CDS             complement(12775..13308)
                     /gene="ymdB"
                     /locus_tag="ACHOSC_RS12420"
                     /old_locus_tag="pgaptmp_002484"
                     /EC_number="3.1.1.106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="O-acetyl-ADP-ribose deacetylase"
                     /protein_id="HGU4548410.1"
                     /translation="MKTRIHVVQGDITKLAVDVIVNAANPSLMGGGGVDGAIHRAAGP
                     ALLDACLKVRQQQGDCPTGHAVITLAGALPAKAVVHTVGPVWRGGEQNEDQLLQDAYL
                     NSLRLVAANSYTSVAFPAISTGVYGYPRAAAAEIAVKTVSEFITRHALPEQVYFVCYD
                     EENAHLYERLLTQQGDE"
     gene            complement(13403..13714)
                     /gene="ymdA"
                     /locus_tag="ACHOSC_RS12425"
                     /old_locus_tag="pgaptmp_002485"
     CDS             complement(13403..13714)
                     /gene="ymdA"
                     /locus_tag="ACHOSC_RS12425"
                     /old_locus_tag="pgaptmp_002485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YmdA"
                     /protein_id="HGU4548411.1"
                     /translation="MFRPFLNSLMLGSLLFPFIAIAGSTAQGGVIHFYGQIVEPACDV
                     STQSSPVEMNCPQNGSVPGKTYSSKALMSGNVKNAQIASVKVQYLDKQKKLAVMNIEY
                     N"
     gene            complement(13835..14167)
                     /gene="csgC"
                     /locus_tag="ACHOSC_RS12430"
                     /old_locus_tag="pgaptmp_002486"
     CDS             complement(13835..14167)
                     /gene="csgC"
                     /locus_tag="ACHOSC_RS12430"
                     /old_locus_tag="pgaptmp_002486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="curli assembly chaperone CsgC"
                     /protein_id="HGU4548412.1"
                     /translation="MNALLLLAALSSQITFNTTQQGDVYTIIPEVTLTQSCLCRVQIL
                     SLREGSSGQSQTKQEKTLSLPANQPIALTKLSLNISPEDRVKIVVTVSDGQSLHLSQQ
                     WPPSSEKS"
     gene            complement(14226..14681)
                     /gene="csgA"
                     /locus_tag="ACHOSC_RS12435"
                     /old_locus_tag="pgaptmp_002487"
     CDS             complement(14226..14681)
                     /gene="csgA"
                     /locus_tag="ACHOSC_RS12435"
                     /old_locus_tag="pgaptmp_002487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="curli major subunit CsgA"
                     /protein_id="HGU4548413.1"
                     /translation="MKLLKVAAIAAIVFSGSALAGVVPQYGGGGNHGGGGNNSGPNSE
                     LNIYQYGGGNSALALQTDARNSDLTITQHGGGNGADVGQGSDDSSIDLTQRGFGNSAT
                     LDQWNGKNSEMTVKQFGGGNGAAVDQTASNSSVNVTQVGFGNNATAHQY"
     gene            complement(14722..15177)
                     /gene="csgB"
                     /locus_tag="ACHOSC_RS12440"
                     /old_locus_tag="pgaptmp_002488"
     CDS             complement(14722..15177)
                     /gene="csgB"
                     /locus_tag="ACHOSC_RS12440"
                     /old_locus_tag="pgaptmp_002488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="curli minor subunit CsgB"
                     /protein_id="HGU4548414.1"
                     /translation="MKNKLLFMMLTILGAPGIAAAAGYDLANSEYNFAVNELSKSSFN
                     QAAIIGQAGTNNSAQLRQGGSKLLAVVAQEGSSNRAKIDQTGDYNLAYIDQAGSANDA
                     SISQGAYGNTAMIIQKGSGNKANITQYGTQKTAIVVQRQSQMAIRVTQR"
BASE COUNT         3779 a         3929 c         3644 g         3899 t
ORIGIN      
        1 tcaatttgtt atttttgtgt tgcaattcat tctccgacac gccattttct ttctctcgca
       61 aattcgtatt ggatgcactt atgtcattca caaatacccc tgaacgctat ggtgttatat
      121 ctgcggcctt ccattggcta agcgcaatta ttgtctacgg catgtttgct ttaggtctgt
      181 ggatggtcac gctcagttat tacgatggct ggtatcacaa agcacccgaa ctgcataaaa
      241 gcatcggtat tttgttaatg atggggctgg ttattcgcgt tctgtggcgt gtcatatctc
      301 caccgccggg tccgctgccg agttattcgc ccatgactcg tcttgccgca aaggctggac
      361 atctcgcatt gtacctattg ctgttcgcca tcggcatcag cggctatctg atctcaactg
      421 ccgatggtaa accgatcagc gtttttggct ggtttgacgt ccccgcgacc cttgccgacg
      481 ctggcgcaca ggcagacttt gccggtgccc tgcatttttg gctcgcgtgg agcgtcgtag
      541 tactgtccgt tatgcacgga tttatggccc tgaagcatca tttcatcgat aaagacgaca
      601 ctctgaagcg catgctggga aagtcgtcat ctgactatgg agtataaaaa atgaaaaaaa
      661 gcctgcttgg tttaaccttc gcgtccctga tgttctctgc cggttcagcg gttgccgccg
      721 attacaaaat tggacaaaga aggtcagcac gcctttgtta acttccgcat ccagcatctt
      781 ggctatagct ggttatatgg cacctttaaa gatttcgacg gtacttttac ctttgacgaa
      841 aaaaatccgg ctgccgataa agtgaatgtg acaattaaca ccaccagcgt cgatactaat
      901 cacgccgaac gcgataaaca tcttcgcagt gcagatttcc tcaataccgc aaaatatcca
      961 caggcaacat tcacctccac cagcgtgaag aaagacggtg acgaactgga tattaccggc
     1021 gatctgacgc tgaatggcgt aaccaaaccc gtcacgctgg aagcgaaatt aattggtcag
     1081 ggcgacgacc catggggtgg taaacgtgca ggcttcgagg ccgaaggcaa aattaagctc
     1141 aaggacttca atatcaagac agatttaggt ccagcttctc aggaagtgga tctgattatt
     1201 tcagtggaag gcgtacagca gaagtaagcg tgaaatgttg ccggatggca cgcatccggc
     1261 aatgatattt attccgttgg atcaggaatg cccagtgttg tattcagacg tccacgagac
     1321 ttattaaaga tcttattgcc attttcacgt cctgcccgac ggcgtcgctg ttcttctggc
     1381 ggtaacgcgc tttcttcgca gcaaatctca ctacaacaac ctttgtattt ttccgcacat
     1441 actggacact gaataaacag caggtggcag ccatcatttt tacagttggt atggctgtcg
     1501 cacggcgcgc cgcactggtg gcaatgcgcg ataatctcat ccgatatacg ttcgcccatc
     1561 cgctcgtcaa aaacaaaatt tttgccaata aaacgcaccg gcaagccctg ctcgcgcgcc
     1621 ttacgggcgt attcaataat tccgccctcg atatgccaga ctttgttgaa tccgttatgt
     1681 ttcatccagg cactggcctt ttcacaacga atgccgccgg tgcagtacat gacgattttt
     1741 ttatctttat gtgcctgcat catctcgact gcttttggca gctgctcacg gaaggtatct
     1801 gccggaattt cgagcgcgtt ttcaaagtgc cccacttcat actcatagtg gttacgcatg
     1861 tcgataaata gtgcatcggg atcgtcaagc atggcgttca cttccgccgc ttgcagatac
     1921 tcgccaacat tgctggcatc aaagtgagga tcgtcaatac cgtcggcaac aatgcgatcg
     1981 cgtaccttca tgcgcagtac ccagaaagat ttcccgtcat catccaacgc gatattcagg
     2041 cgtaaaccct ctaaagccgg gtcgaaggca tagagctgcg cgcgaaatgt ttcaacattg
     2101 ctcgcaggta cgctgatttg cgcgttaatg ccctcatgcg ccagatacac tcgcccaaaa
     2161 acattcagcg cggtaaacag ctgatataaa gcgtcacggg tcgccttagg atcggcgatg
     2221 tggaaatact tgtaaaacga aatggtggtt cgcggttcgc tctcagccaa cattttggct
     2281 tttagcgcgt cgttggaaat gcggttgtgt aacactggca tggtgtacgg ttcctgcgag
     2341 atgggaaagt aaaaatccgc ggcatgatat agcaattatc gataattaac atccacacat
     2401 tttacgctac atttgcgcat taaaaattat ttgttattta caagtgcggc aatttcgccc
     2461 agtcttcagc cacaaatttt ggttgcgggc gaaaaaatgc gacaatacat acaattgccc
     2521 gaataggttg aaaaacagga ttgatatgac gaatctaccc aagttctcca ccgcactgct
     2581 tcatccgcgt tattggttaa cctggttggg tattggcgta ctttggttag tcgtgcaatt
     2641 gccctacccg gttatctacc gcctcggttg tggattagga aaactggcgt tacgttttat
     2701 gaaacgacgc gcaaaaattg tgcatcgcaa cctggaactg tgcttcccgg aaatgagcga
     2761 acaagaacgc cgtaaaatgg tggtgaagaa tttcgaatcc gttggcatgg gcctgatgga
     2821 aaccggcatg gcgtggttct ggccggaccg ccgaatcgcc cgctggacgg aagtgatcgg
     2881 catggaacac attcgtgacg tgcaggcgca aaaacgcggc atcctgttag ttggcatcca
     2941 ttttctgaca ctggagctgg gtgcgcggca gtttggtatg caggaaccgg ggattggcgt
     3001 ttaccgcccg aacgataatc cactgattga ctggctacaa acctggggcc gtttgcgctc
     3061 aaataaatcg atgctcgacc gcaaagattt aaaaggcatg attaaagccc tgaaaaaagg
     3121 cgaagtggtc tggtacgcac cggatcatga ttacggcccg cgctcaagcg ttttcgtccc
     3181 gttatttgcc gttgagcagg ctgcgaccac gaccggaacc tggatgctgg cacggatgtc
     3241 cggcgcatgt ctggtgccct tcgttccacg ccgtaagcca gatggcaaag ggtatcaatt
     3301 gattatgctg ccgccagagt gttctccgcc actggatgat gccgaaacta ccgccgcgtg
     3361 gatgaacaaa gtggtcgaaa aatgcatcat gatggcacca gagcagtata tgtggttaca
     3421 ccgtcgcttt aaaacacgcc cggaaggcgt tccttcacgc tattaaatct cccctgccgg
     3481 atgcttcaga atggcatccg gcattaccac agcaaatccc cctgatttag cgataaaagc
     3541 tctctggatt gcgccccctg gaagtcgagc gcataattag tgtgcttatc ttttcttctt
     3601 atgttcaccg cgcctggcgc accaacagcg gattgctatg tcaccctgtg aaaatgacac
     3661 ccctataaac tggaaacgaa acctgatcgt cgcctggcta ggctgttttc ttaccggtgc
     3721 cgccttcagt ctggtaatgc ccttcttacc cctctacgtt gagcagcttg gcgttaccgg
     3781 tcactccgcc ctgaatatgt ggtccggtat tgtcttcagc attacatttt tattttcggc
     3841 catcgcctca ccgttttggg gtggactcgc cgaccgtaaa ggccgaaaac tcatgctatt
     3901 acgctctgcc ctcggcatgg gcatcgtgat ggtgttgatg ggactggcac aaaatatctg
     3961 gcagtttttg atcctacggg cgcttcttgg gttacttggc ggatttgtcc ccaacgctaa
     4021 tgctcttatc gccacacaag taccgcgtaa taaaagcggc tgggcgctgg gtacgctctc
     4081 cacaggcggc gttagtggtg cgttgctcgg cccaatggct ggcggcctgc tcgccgatag
     4141 ctacggctta cgtccggtat tctttattac cgccagtgtg ctcatactct gctttttcgt
     4201 caccctgttc tgcatcagag aaaaattcca gccggtcagc aaaaaagaga tgctgcacat
     4261 gcgggaagtg gtgacatcac ttaaaaaccc gaaactggta ctcagcctgt ttgtcaccac
     4321 gttaatcatc caggtggcga caggctcaat tgcccccatt ctgacgctgt atgtccgcga
     4381 actggcgggt aacgtcagta acgtcgcctt tatcagtggc atgatcgcct cggtgccagg
     4441 cgtggcggct ctgctaagcg caccacgact cggcaaactt ggcgatcgaa tcggacccga
     4501 aaagatcctg attacagcgc tgatcttttc tgtactgctg ttgatcccaa tgtcttacgt
     4561 tcagacgcca ttgcaacttg ggattttacg ttttttgctc ggtgccgccg atggtgcact
     4621 actccccgcc gtacagacac tgttggttta caactcgagc aaccagatcg ccgggcgtat
     4681 cttcagctat aaccaatcgt ttcgtgatat tggcaacgtt accggaccat tgatgggagc
     4741 agcgatttca gcgaactacg gtttcagagc ggtatttctc gtcaccgctg gcgtagtgtt
     4801 attcaacgca gtctattcat ggaacagtct acgtcgtcgt cgaatacccc aggtatcgaa
     4861 ctgatttttc gcctttcata cttgcaaaag cggagaatca gctatccttt tccctgaaac
     4921 ctcatcaact caaagggaga atcgtgatga ccatgtacgc aacgcttgaa gaagccattg
     4981 acgctgcacg cgaagaattt cttgcagaca accccggcat cgacaccgaa gatgcgaatg
     5041 tgcaacagtt caatgcccaa aaatacgttt tgcaggacgg cgacatcatg tggcaagttg
     5101 agttttttgc cgacgaaggg gaagaaggtg aatgtttacc tatgcttagc ggtgaagccg
     5161 cgcaaagtgt ttttgatggc gactatgatg agatagagat acgccaggag tggcaggaag
     5221 agaatacatt acatgaatgg gacgaggggg aatttcagct tgagccaccg ctggataccg
     5281 aggaaggacg cgcagcagct gatgagtggg atgaacgtta atcactcata cgggccatga
     5341 tgaatgtcga tcggcagcag taaagtatca aaaaccagag agaatggcag atcaaggatc
     5401 gtgacataac gccaggcgga gtcacgcaca tcccattgca caccgggata atattggttg
     5461 ccatgcccct gccccggtat agtgcgacta ataatgctgc cacagccact cagcaaggtc
     5521 accatgatgc tcaccacaat taaacgcatt tgatacctct ttcgtaaaaa aataccggca
     5581 taacgccggt atttttattg gctcgttgtt tttgccggat gcggcgtgaa cgccttatcc
     5641 gacccacaaa atcttgcaaa atcaataaat tgcaggaacg atgtaggcct gataagcgta
     5701 gcgcatcagg caactacgtt tttattgcga agccgcatcc ggtttacgca atgccagtgg
     5761 attgagcttt atcccttcgt aataactcac ccatgaagaa tatctctccg gggaattcca
     5821 gacgcggaaa tgcagacggg ccatcgtcac cggatcgctt agcagcacca ggcgacgatc
     5881 gcgattcagc ttctctggcg tctcgttcag cgcctgttca acgtggcggt cacgggcgat
     5941 ttccagcacc ttactggcgc ggtgacgcgc ggtcgccatt gcggttgcca gagcgttaaa
     6001 tgacgggtta aacactgcgt gcataaagcc atcatcaagg gagcgttgac gattcatctc
     6061 aaggaaccga tcggtatcaa ccagcacctg cggcggcgaa tactcttccg ggatcaggaa
     6121 cagtttccag cgtttggtgc gcagaccaac ggtggcacgg ctggaaatca ccgaaacaaa
     6181 cggtgacagg atcaacgaga agacaatcgg tgccagccag aacaggaaac gcagatccaa
     6241 ccacgccata ccaacagccc acactaaccc taacagcagc tgtgagccgt ggcgtttgaa
     6301 cgcttcaccc caggaagtgg agtcatcatc acgctgcggt gaattccaca ccacttccca
     6361 gccaaggaac gcgctgacga cgaagaccgt atggaacagc atgcgtaccg gagccagcag
     6421 cacggaaaac agcacttcca gcagcaacga taatgtaacg cgccagaagc cgccgtattc
     6481 tttcgttcct ttgcaccaga taagcaaaat gctcaataac ttcggcagga acaacagcac
     6541 catggtcgaa gcaaaaagtg caatcgccag ctcaggacgc cactgcggcc acaccgggaa
     6601 caactgccgt ggttgcagga agtattgcgg ttcggtcaac gcatgtacta cctgcaatgc
     6661 agtagagagc gcgaggaaca taaaccacag cggagcggag agataagaca tcacgcccgt
     6721 caggaatacc gcacggtgaa ccgggtgcat acccttcacc aggaacagac ggaagttcat
     6781 caggttaccg tggcaccagc ggcggtcacg ttttagctca tcaagcaagt taggcggcaa
     6841 ttcttcataa gaacccggga gatcgtaagc aatccagacc ccccaacctg cacggcgcat
     6901 caacgccgct tccacgaagt catgtgacag gattgaaccg gcaaaggaac cttcgcccgg
     6961 cagcggagcc agtgcgcagt gctcgataaa cggtttcacg cggataatcg cgttatgtcc
     7021 ccagtagtgc gactcgccaa gttgccagaa gtgcaaaccg gctgtaaaca gtggcccata
     7081 cacgcgggtc gcgaactgct gacagcgcgc atacagcgta tccataccgg acgctttcgg
     7141 cgacgactga atgatcccgg cgttcgggtt ggcttccatc aggcgcacca ggccgcataa
     7201 acaatcaccg gtcattaccg agtcagcatc cagcaccacc atgtagctgt actggctgcc
     7261 ccagcgacgg cagaagtcat cgatattacc gcttttacgc ttcacgcggc gacggcggcg
     7321 gcgatagaaa atctgacctt cgccaccgac ttcagcgata agctccatcc aggctttttg
     7381 ctctgcgacg cagatatccg ggttataact gtcactaaga atgtagacat caaagtgttt
     7441 ggcattcccg gtggctttta ctgattccca cgttgcacgc aggccagcaa aaacacggtt
     7501 cacgtcttcg ttacagatag gcatgatcaa cgccgtgcga tgctccgggt ttaatggttc
     7561 atcgccaact gttgacgcag atatactgta tttatcgcga ccaataagca gttgcaggaa
     7621 gcccattaac gccgtccaga atccggcgga cacccaacag aacagtaccg caaagaggat
     7681 caggataccg gtttgcagca tataaggcag aagctgcata aaggaaaccc acaaatcctg
     7741 accaaccata tccataggat taatcagcgc ccaaccctga taaggaagaa tggtcttcat
     7801 ataccaggtc gcgacgacag tttgcgcgag cgtcaggatc aacagaatgt aacggcggat
     7861 ggtaccgacg gtacgccact tttgctcgct ctcctgctct tctttggtca aacgagccag
     7921 atagcgcggc gtgacatcgc gtccacgcag gcgatcccag aaacggccta ccgggttggt
     7981 acgccacggg tcgggaaaca tcgaggagcg ttttgcttct ggcatcgcct tcagctgatc
     8041 gcgcccttcg tcgtctttaa ttaactgtcc atcagcaagt gaatctggcc aggcttgttc
     8101 cagacgcgcc tttaccgagc cttgcgggga atcatcctcc cgcgcccagg tgcggtgttc
     8161 ggcatccagc gcctgatgaa cggcgcggat atcagtcttc ggcaatgccg ctttctcgct
     8221 tgcggcgatg ggcattgcgt caatgtactc agttgtctta ttcattggca ggtaactggt
     8281 agctccaggt ttcactcaac gtctgatcgg cattcaccag cgcagcacgc atttcagtgg
     8341 ttttcttggc atctttcact ttcacacgca tcaccagacg ccagcctttg gtaaccgggt
     8401 tatagcgcac cgtgctttca actatctcac cattatcacc aatgctggtt tgcgctgtga
     8461 ccggggtatc ctctggcagt tttttcatct ctgcgccggt aaaatcgacc acaaaggcga
     8521 tagtaccgtc aggctggcga atcaggttcg actgcttcac atcccccgtt gaacgacgcg
     8581 tttgttgcac ccatgcgtta tctggcgcat gcagtttgtc ttcatcacgg ctgaaggtga
     8641 tggtgtattt aaagttcatc tctttacccg gctccggcag ctgatccggc gtccagtaag
     8701 cgacgatgtt atcgttggtt tcatcgttgg ttggaatttc caccagctca acgctgcctt
     8761 taccccactc ccctttcgga gtcacccatg cgcttggacg aagatcgtaa cgatcatcga
     8821 gatcttcaaa gcgggagaaa tcacgaccgc gctgcaacag accgaagcct tgcgggttct
     8881 ccatcgagaa gctgctgacc gctaaatgtt tcgggttatt caacggacgc cagatccatt
     8941 cgccattacc ggcatggata gacagaccgt tagagtcgtg caactccgga cgatagttat
     9001 ttgcaggcga cggttggttc ggcccaaaca ggaacatact ggttaacggt gcaaccccca
     9061 gtttgccgac tttatcgcgc agatagattt tcgactgcac atccacaacc gtgtcacgcc
     9121 ctggcataac cacgaatttg taagcacctg tcgcgcgcgg cgagtcaagc aatgcataaa
     9181 tggttaaacg tttatcagtc ggttttggac gctcgatcca gaactctttg aagcgtggaa
     9241 attcttcacc cgatggcaag gcggtatcaa ttgccaggcc gcgggcagaa aggccataaa
     9301 cctgacctgc accaatcacg cggaaatagc tggccccgag catgctgacg atttcatcgt
     9361 ttttatcttt gctgttgatc gggtaaagca ctttaaaacc ggcaaaacca aggtctttta
     9421 ccgtgtcttt gtcatgctga acatcgccga aagtgaaata atccgggctg tatttgattc
     9481 gtttgactgc ggtggcagtc acttcattta ttttgaccgg ggtatcgaag tacataccct
     9541 gatggtagaa ctcgagtttg aatggggtct tcagattgtt ccagtacgct ttgtcatgat
     9601 taaactggat ctgctgatag tccgcgtatt tcatatcgcg gaaaacggag ggcaagttgc
     9661 ttttgggcgc ctcatagcct ttcccggcta aggattgagc ttgctttgcg acatcatcaa
     9721 tactgaaagc ccagcttgaa gatgtataca gggttaacat tactgcagca ctcaaccaac
     9781 gcattttcat catttgtagt ttatgtttca taattagtaa gcacttcccc ctttgtgtgc
     9841 ttatatcgat ccgatctatt ttaatggata accagccatt ccgacaacag aatcccggta
     9901 tcgttcagca cgctggctca tggattaagc acctgattgc aaccgttttc actttccgtg
     9961 cttctcctaa agacagaagc atcgacttcg tgtagggttt gccagcaatt ttatttatca
    10021 accgtcttac agacgcttcc attctggtta gtggtagcga tgatgctatg cataaaaacc
    10081 ttgaagcctg cgcctggcgc ggtcaggtcg gtgtcgctat ggcaacaagc ctaaaggcat
    10141 aacccgacag ggataagacg aaaaatccga gattagttaa ccatatatga atccagtacc
    10201 cgcgcaacgt gaatatttcc tcgactccat ccgcgcctgg ctgatgttgt tagggatccc
    10261 ttttcatatt tctttaatct attcgagcca tacatggcat gtgaatagcg ccgaaccatc
    10321 attgtggctg acccttttta atgacttcat ccactcgttc cgcatgcagg tatttttcgt
    10381 tatatccggc tacttttcct acatgctttt tttacgctat cccttgaaaa aatggtggaa
    10441 agtacgtgtc gaacgtgtag gtatcccgat gttaacagcc atccccctac tgacattacc
    10501 gcaatttatt atgctgcaat atgtcaaagg aaaagcggaa agttggcctg ggctgtcatt
    10561 gtatgacaaa tataatacgt tggcctggga attaatatca cacctgtggt ttttactggt
    10621 gttagtagtc atgacgacgc tgtgcgtatg gatatttaag cgcatcagaa ataatttaga
    10681 aaattctgat aaaacgaata aaaaattctc gatggtaaaa ctatcggtga tttttttatg
    10741 cctcggcatc ggttatgcgg taataagaag aacgattttt attgtgtatc cgcccattct
    10801 gagtaatggc atgttcaatt ttattgtcat gcaaacgctg ttttatttgc cgttctttat
    10861 cctcggcgca ctggctttca ttttccctca tcttaaagcc ttgtttacca cgccgtctcg
    10921 tggctgtacc cttgcagcag cattggcgtt tgtcgcttat ttactcaacc agcgctatgg
    10981 cagtggcgat gcctggatgt acgaaaccga gtcggtgatc accatggtcc tcggtctgtg
    11041 gatggtgaat gtggtcttct cctttggcca ccgtttgctt aacttccagt cagcgcgggt
    11101 gacttatttt gttaacgcat cgctgtttat ctatctggtt caccacccgt taacgctgtt
    11161 tttcggcgca tacattacac cgcacatcac ctccaactgg cttggttttc tctgtggcct
    11221 gatatttgta gtagggattg cgataattct gtatgaaatt catttgcgca tcccgttact
    11281 gaagtttttg ttctctggta aaccggttgt taagcgtgag aacgataaag caccagcccg
    11341 ttaagccaca tttacaataa ccattccacg ggcaatatcg acgccagtct gaccataacc
    11401 cgcttccaga aactggtggc gggttctttt ttgagaataa tctctttctt tgcatgacga
    11461 tcaacccagt tgatccgtcc ccacctgtcc agacggagct gccaggccgc atcatactgg
    11521 ctctgaataa agcgtttatc aattaactgt gccagcgttt cgctctctat cacgaagccc
    11581 atttcagtat tgagcaatgt tgaacgcgga tcgaaattga aagagccgat aaacaccgtt
    11641 ttaccatcga tgctaaaggt tttagcatgc aggctggctc cggaattacc ggttatgccg
    11701 cgatcgtgta acgtactact ttgttcacgc gtcggcttga gttcatataa ttccacgcca
    11761 tagcggagca attttttgcg ccagcgcgcg tatccggcat ggacgacagc aacatcgtta
    11821 gcggcaagag aattggttag gatcgcaatc tttacccctt ttctcaccat ccgtaagagt
    11881 tgcgccacac ctgcgcgtgt cggtacaaaa taggaagaga taatatcgat gcgttcactg
    11941 ggtgagccca tgatatcgaa caggcgctgc ggtaacagtg aatgccgttt tgccttgccc
    12001 tcccctttcg ccggatcatc acttaataaa cgtgttttcg cccagataag cggcaacgtt
    12061 ccatcaacca gatgatttat aaacggactg gattccattt tgcgtaagta acgatgcgtc
    12121 atagcatcgt tatgccagga ggcgggtaac tcgatgcgat ccgccatttc accttccggg
    12181 acatccagca cttgctgtaa gggtgaaacc gatttgcaat accagtagcg ggcgaaatca
    12241 tcggcaacgt cctctaccac gggccctatt gccataacat ctaaatccga aaaaagtggc
    12301 tcttcccctg ccccaaaata ggcatcacca atatttcgcc cccctaccag cgtcaccacg
    12361 ccatcgacag tgaaactttt attgtgcata cggcgattga gacgggaaaa gtcggtgata
    12421 taaccaagcg gacgcagcaa gcgaaacgag aaaggattaa aaagccggac ttcaatgcgg
    12481 ggatggctgt caagcaagcg taaaatgtcg tcaagtccgg gcgtattgtt gtcgtccagc
    12541 aacaaacgga cgcgaacgcc acgctttgct gcggctaaca gggcggaaaa cagtaaccga
    12601 cccgacatat cgtcctgcca gatgtaatac tgaacatcca gcgtatgctc tgccatttcg
    12661 gccagacgat accgggcggc aaacgcatcc agacttttct ccagagggaa aagaccacac
    12721 tgaccgggat gttgcgaaca cagtggcagc accgcgctcg ccagccgggg caaatcattc
    12781 atctccttgt tgggtaagga gtctttcgta gaggtgggcg ttttcttcat cataacagac
    12841 aaagtatacc tgttcgggta aagcgtgacg ggtaataaat tctgaaacgg ttttaactgc
    12901 gatttcagcc gctgccgcac gagggtaacc ataaacccca gtactgattg caggaaaagc
    12961 cactgacgta tagctgtttg ccgccaccag tcgtaggcta ttgagatagg catcctgcaa
    13021 aagctggtct tcgttttgtt caccaccacg ccagactggc ccgacggtgt gcactacggc
    13081 tttagcggga agagcgcctg caagcgtaat aacggcatgc cccgtagggc aatcgccctg
    13141 ctgttgcctg acttttaaac aggcatccag cagggccgga cccgctgcgc gatgaatggc
    13201 cccatcgacg ccgccgcctc ccattaatga cggattagcc gcattcacaa tcacatcaac
    13261 ggccagtttg gtaatatcac cctgcacaac atgaatacgc gttttcataa cgcctcctta
    13321 caccttcttg tttcttaagt gtacagcagg ctttttggtt aaatcgcatt tttctgctca
    13381 gcaacgtgcg ttgttcagaa acttagttat attcgatgtt cattacagcc agctttttct
    13441 gtttatcaag atactgcact tttactgagg ctatttgcgc gtttttaaca ttaccgctca
    13501 ttaacgcttt gctggaatag gttttgcccg gaacagaacc attctgtggg cagttcattt
    13561 ctacgggtga tgactgggtg ctgacgtcac atgccggttc cacaatttgg ccataaaaat
    13621 ggatcacccc cccttgcgcg gtgcttcctg cgatggcaat aaaaggaaaa agcaaactgc
    13681 cgagcataag agagtttaaa aaaggtctga acatttattc tatttcctca atgattagtc
    13741 atccttgagg gttgtgttat ccatactttc aggttggctg attcgcagca gaccattctc
    13801 tccagattca tcttatgctc gatatttcaa caaattaaga cttttctgaa gagggcggcc
    13861 attgttgtga taaatgaagt gactgtccat cagaaacagt aacaactatt ttcacccgat
    13921 cttccgggga aatatttaaa ctcaacttcg tcaaagcaat aggttgatta gcaggcaatg
    13981 agagggtctt ttcttgcttc gtctgacttt gccctgaact gccttcgcgc agggacaata
    14041 tttgtactct gcacagacaa gattgagtaa gagtgacttc aggaataatg gtatacacat
    14101 ccccttgctg ggtcgtatta aaggttatct ggctggaaag tgccgcaagg agtaataacg
    14161 cattcatatt cttctcccga aaaaaaacag ggcttgcgcc ctgtttctgt aatacaaatg
    14221 atgtattagt actgatgagc ggtcgcgttg ttaccaaagc caacctgagt cacgttgacg
    14281 gaggagttag atgcagtctg gtcaactgca gcaccgttgc caccaccgaa ctgtttaaca
    14341 gtcatttcag aatttttgcc gttccactga tcaagagtgg cgctgttacc gaagccacgt
    14401 tgggtcagat cgattgagct gtcatctgag ccctgaccta catctgcacc attaccaccg
    14461 ccatgctggg taatagtcaa gtcagagtta cgggcatcag tttgcagagc aagtgcagag
    14521 ttaccgccac cgtactggta aatgttcagc tctgaatttg ggccgctatt attaccgcca
    14581 ccaccgtggt taccgccgcc gccgtactga ggaacaacac ctgccagagc gctaccggag
    14641 aatacgattg ctgcaattgc tgctactttt aaaagtttca tgtaaaaccc ccatcggatt
    14701 gatttaaaag tcgaatggaa attaacgttg tgtcacgcga atagccattt gcgactgtct
    14761 ctgcactaca attgccgttt tttgagtacc atactgtgta atatttgctt tattaccaga
    14821 acctttctgg ataatcatcg cagtattacc ataagcacct tgcgaaatac tggcatcgtt
    14881 ggcactgccc gcctgatcaa tatatgcaag gttataatct cctgtctggt caatctttgc
    14941 ccggttgcta ctaccttctt gcgcaacaac cgccaaaagt tttgagcctc cctgccgtaa
    15001 ctgagcacta ttattcgtcc cagcttgacc aattatggct gcctgattaa atgaagactt
    15061 actcaattca tttaccgcga agttatattc tgaattagct aaatcataac ctgctgcggc
    15121 tgcaatccca ggcgcaccca gtattgttaa catcataaat aacaatttgt ttttcatgtt
    15181 gtcaccctgg acctggtcgt acatttaaga aattaaatca tttcaacttg gttgttaacg
    15241 caacctgtat t
//
LOCUS       DAZKHF020000098        15212 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2201S31241
            SAMN43267800-rid25106413.denovo.099, whole genome shotgun sequence.
ACCESSION   DAZKHF020000098 DAZKHF020000000
VERSION     DAZKHF020000098.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267800
            Sequence Read Archive: SRR30307263
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 15212)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15212)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 15212)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350581.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350581.2
            Genome Coverage       :: 151x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:21:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,652
            CDSs (total)                      :: 5,557
            Genes (coding)                    :: 5,320
            CDSs (with protein)               :: 5,320
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 237
            CDSs (without protein)            :: 237
            Pseudo Genes (ambiguous residues) :: 0 of 237
            Pseudo Genes (frameshifted)       :: 102 of 237
            Pseudo Genes (incomplete)         :: 130 of 237
            Pseudo Genes (internal stop)      :: 52 of 237
            Pseudo Genes (multiple problems)  :: 41 of 237
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15212
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267800-rid25106413.denovo.099"
                     /serotype="O 26:H11"
                     /isolate="2201S31241"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-01"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            complement(219..1043)
                     /gene="iclR"
                     /locus_tag="ACHOSC_RS12445"
                     /old_locus_tag="pgaptmp_002489"
     CDS             complement(219..1043)
                     /gene="iclR"
                     /locus_tag="ACHOSC_RS12445"
                     /old_locus_tag="pgaptmp_002489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418442.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glyoxylate bypass operon transcriptional
                     repressor IclR"
                     /protein_id="HGU4548415.1"
                     /translation="MVAPIPAKRGRKPAVATAPATGQVQSLTRGLKLLEWIAESNGSV
                     ALTELAQQAGLPNSTTHRLLTTMQQQGFVRQVGELGHWAIGAHAFMVGSSFLQSRNLL
                     AIVHPILRNLMEESGETVNMAVLDQSDHEAIIIDQVQCTHLMRMSAPIGGKLPMHASG
                     AGKAFLAQLSEEQVTKLLHRKGLHAYTHATLVSPVHLKEDLAQTRKRGYSFDDEEHAL
                     GLRCLAACIFDEHREPFAAISISGPISRITDDRVTEFGAMVIKAAKEVTLAYGGMR"
     gene            1243..4926
                     /gene="metH"
                     /locus_tag="ACHOSC_RS12450"
                     /old_locus_tag="pgaptmp_002490"
     CDS             1243..4926
                     /gene="metH"
                     /locus_tag="ACHOSC_RS12450"
                     /old_locus_tag="pgaptmp_002490"
                     /EC_number="2.1.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0008705 - methionine synthase activity
                     [Evidence IEA];
                     GO_function: GO:0031419 - cobalamin binding [Evidence
                     IEA];
                     GO_process: GO:0009086 - methionine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine synthase"
                     /protein_id="HGU4548416.1"
                     /translation="MSSKVEQLRAQLNERILVLDGGMGTMIQSYRLNEADFRGERFAD
                     WPCDLKGNNDLLVLSKPEVIAAIHNAYFEAGADIIETNTFNSTTIAMADYQMESLSAE
                     INFAAAKLARACADEWTARTPEKPRYVAGVLGPTNRTTSISPDVNDPAFRNITFDQLV
                     AAYRESTKALVEGGADLILIETVFDTLNAKAAVFAVKTEFEALGVELPIMISGTITDA
                     SGRTLSGQTTEAFYNSLRHAEALTFGLNCALGPDELRQYVQELSRIAECYVTAHPNAG
                     LPNAFGEYDLDADTMAKQIREWAQAGFLNIVGGCCGTTPQHIAAMSRAVEGLAPRKLP
                     EIPVACRLSGLEPLNIGEDSLFVNVGERTNVTGSAKFKRLIKEEKYSEALDVARQQVE
                     NGAQIIDINMDEGMLDAEAAMVRFLNLIAGEPDIARVPIMIDSSKWDVIEKGLKCIQG
                     KGIVNSISMKEGVDAFIHHAKLLRRYGAAVVVMAFDEQGQADTRARKIEICRRAYKIL
                     TEEVGFPPEDIIFDPNIFAVATGIEEHNNYAQDFIGACEDIKRELPHALISGGVSNVS
                     FSFRGNDPVREAIHAVFLYYAIRNGMDMGIVNAGQLAIYDDLPAELRDAVEDVILNRR
                     DDGTERLLELAEKYRGSKTDDTANAQQAEWRSWEVNKRLEYSLVKGITEFIEQDTEEA
                     RQQATRPIEVIEGPLMDGMNVVGDLFGEGKMFLPQVVKSARVMKQAVAYLEPFIEASK
                     EQGKTNGKMVIATVKGDVHDIGKNIVGVVLQCNNYEIVDLGVMVPAEKILRTAKEVNA
                     DLIGLSGLITPSLDEMVNVAKEMERQGFTIPLLIGGATTSKAHTAVKIEQNYSGPTVY
                     VQNASRTVGVVAALLSDTQRDDFVARTRKEYETVRIQHGRKKPRTPPVTLEAARDNDF
                     AFDWQAYTPPVAHRLGVQEVEASIETLRNYIDWTPFFMTWSLAGKYPRILEDEVVGVE
                     AQRLFKDANDMLDKLSAEKTLNPRGVVGLFPANRVGDDIEIYRDETRTHVINVSHHLR
                     QQTEKTGFANYCLADFVAPKLSGKADYIGAFAVTGGLEEDALADAFEAQHDDYNKIMV
                     KALADRLAEAFAEYLHERVRKVYWGYAPNENLSNEELIRENYQGIRPAPGYPACPEHT
                     EKATIWELLEVEKHTGMKLTESFAMWPGASVSGWYFSHPDSKYYAVAQIQRDQVEDYA
                     RRKGMSVSDVERWLAPNLGYDAD"
     gene            5146..6777
                     /locus_tag="ACHOSC_RS12455"
                     /old_locus_tag="pgaptmp_002491"
     CDS             5146..6777
                     /locus_tag="ACHOSC_RS12455"
                     /old_locus_tag="pgaptmp_002491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005436 - sodium:phosphate symporter
                     activity [Evidence IEA];
                     GO_process: GO:0044341 - sodium-dependent phosphate
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Na/Pi cotransporter family protein"
                     /protein_id="HGU4548417.1"
                     /translation="MLTLLHLLSAVALLVWGTHIVRTGVMRVFGARLRTVLSRSVEKK
                     PLAFCAGIGVTALVQSSNATTMLVTSFVAQDLVALAPALVIVLGADVGTALMARILTF
                     DLSWLSPLLIFIGVIFFLGRKQSRAGQLGRVGIGLGLILLALELIVQAVTPITQANGV
                     QVIFASLTGDILLDALIGAMFAIISYSSLAAVLLTATLTAAGIISFPVALCLVIGANL
                     GSGLLAMLNNSAANAAARRVALGSLLFKLVGSLIILPFVHLLAETMGKLSLPKAELVI
                     YFHVFYNLVRCLVMLPFVDPMARFCKTIIRDEPELDTQLRPKHLDVSALDTPTLALAN
                     AARETLRIGDAMEQMMEGLNKVMHGEPRQEKELRKLADDINVLYTAIKLYLARMPKEE
                     LAEEESRRWAEIIEMSLNLEQASDIVERMGSEIADKSLAARRAFSLDGLKELDALYEQ
                     LLSNLKLAMSVFFSGDVTSARRLRRSKHRFRILNRRYSHAHVDRLHQQNVQSIETSSL
                     HLGLLGDMQRLNSLFCSVAYSVLEQPDEDEGRDEY"
     gene            complement(6868..7557)
                     /gene="pepE"
                     /locus_tag="ACHOSC_RS12460"
                     /old_locus_tag="pgaptmp_002492"
     CDS             complement(6868..7557)
                     /gene="pepE"
                     /locus_tag="ACHOSC_RS12460"
                     /old_locus_tag="pgaptmp_002492"
                     /EC_number="3.4.13.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptidase PepE"
                     /protein_id="HGU4548418.1"
                     /translation="MELLLLSNSTLPGKAWLEHALPLIAEQLQGRRSAVFIPFAGVTQ
                     TWDDYTAKTAAVLAPLGVSVTGIHSVVDPVAAIENAEIVIVGGGNTFQLLKQCRERGL
                     LAPITDVVKRGALYIGWSAGANLACPTIRTTNDMPIVDPQGFDALNLFPLQINPHFTN
                     ALPEGHKGETREQRIRELLVVAPELTIIGLPEGNWITVSKGHATLGGPNTTYVFKAGE
                     EAVPLEAGHRF"
     gene            complement(7940..9169)
                     /gene="sorE"
                     /locus_tag="ACHOSC_RS12465"
                     /old_locus_tag="pgaptmp_002493"
     CDS             complement(7940..9169)
                     /gene="sorE"
                     /locus_tag="ACHOSC_RS12465"
                     /old_locus_tag="pgaptmp_002493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-sorbose 1-phosphate reductase"
                     /protein_id="HGU4548419.1"
                     /translation="MKTTALRLYGKRDLRLETFDLPEMQEDEILATVVTDSLCLSSWK
                     EANLGENHKKVPDDVATNPIIIGHEFCGDILAVGKKWQHKFQPGQRYVIQANLQLPDR
                     PDCPGYSFPWVGGEATHVVIPNEVMEQDCLLAYDGETYFEGSLVEPLSCVIGAFNANY
                     HLQEGSYNHTMGIRPQGRMLILGGTGPMGLLAIDYALHGPVNPSLLVITDTDNDKLSY
                     ARKHYPSEPQTLIHYLNAADAAFDTLMALSGGHGFDDIFVFVPNEGLVTLASSLLATD
                     GCLNFFAGPQDKHFSAPINFYDVHYAFTHYVGTSGGNTDDMRAAVKLIEEKKVQAAKV
                     VTHILGLNAAGETTLELPAVGGGKKLVYTGKYLPLTSLTQIQDQALAAILARHQGIWS
                     GEAEQYLLTHAEAISHD"
     gene            complement(9221..10044)
                     /locus_tag="ACHOSC_RS12470"
                     /old_locus_tag="pgaptmp_002494"
                     /pseudo
     CDS             complement(9221..10044)
                     /locus_tag="ACHOSC_RS12470"
                     /old_locus_tag="pgaptmp_002494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709807.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
     gene            complement(10055..10852)
                     /locus_tag="ACHOSC_RS12475"
                     /old_locus_tag="pgaptmp_002495"
     CDS             complement(10055..10852)
                     /locus_tag="ACHOSC_RS12475"
                     /old_locus_tag="pgaptmp_002495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005224538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0034219 - carbohydrate transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose/fructose/sorbose transporter subunit
                     IIC"
                     /protein_id="HGU4548420.1"
                     /translation="MEISTLQIIAIFLFSCIAGMGSVLDEFQTHRPLIACTVIGLILG
                     DLKTGIILGGTLELIALGWMNVGAAQSPDSALASIISAILVIVGQQSIATGIAIALPV
                     AAAGQVLTVFARTITVVFQHAADKAAEEARFRTLDILHVSALGVQALRVAIPALIVSL
                     FVSADMVSNMLSAIPEFVTRGLQIAGGFIVVVGYAMVLRMMGVKYLMPFFFLGFLAGG
                     YLDLSLLAFGGVGVIMALVYIQLNPQWRKAEPQPQATTSTALDQLDD"
     gene            complement(10918..11412)
                     /locus_tag="ACHOSC_RS12480"
                     /old_locus_tag="pgaptmp_002496"
     CDS             complement(10918..11412)
                     /locus_tag="ACHOSC_RS12480"
                     /old_locus_tag="pgaptmp_002496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system
                     mannose/fructose/N-acetylgalactosamine-transporter subunit
                     IIB"
                     /protein_id="HGU4548421.1"
                     /translation="MNITLARIDDRLIHGQVTTVWSKVANAQRIIICNDEVYNDEVRR
                     TLLRQAAPPGMKVNVVNIEKAVAVYHNPQYQDETVFYLFTRPQDALAMVRQGVKIDTL
                     NIGGMAWRPGKKQLTKAVSLDDDDINAFHELNNLGVILDLRVVASDPSINIIDKINEQ
                     LIAN"
     gene            complement(11412..11819)
                     /locus_tag="ACHOSC_RS12485"
                     /old_locus_tag="pgaptmp_002497"
     CDS             complement(11412..11819)
                     /locus_tag="ACHOSC_RS12485"
                     /old_locus_tag="pgaptmp_002497"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0016773 - phosphotransferase activity,
                     alcohol group as acceptor [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannose/fructose/sorbose PTS transporter subunit
                     IIA"
                     /protein_id="HGU4548422.1"
                     /translation="MVNAIFCAHGKLACAMLESVQMVYGNANVEAVEFVPGENAGDIV
                     AKLEKLVSIHNQDEWLIAVDLQCGSPWNAAAMLAMRNPRLRVISGLSLPLALELVDNQ
                     DSMNVDELCEHLTQIAKQTCVVWKQLATAEEDF"
     gene            complement(11829..12635)
                     /locus_tag="ACHOSC_RS12490"
                     /old_locus_tag="pgaptmp_002498"
     CDS             complement(11829..12635)
                     /locus_tag="ACHOSC_RS12490"
                     /old_locus_tag="pgaptmp_002498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HGU4548423.1"
                     /translation="MQTWLNLQDKIIIVTGGASGIGLAIVEELLAQGANVQMVDIHGG
                     DGQYEGHKGYQFWPTDISSAKEVNHTVAEIIQRFGRIDGLVNNAGVNFPRLLVDEKAP
                     AGQYELNEAAFEKMVNINQKGVFLMSQAVARQMVKQHDGVIVNVSSESGLEGSEGQSC
                     YAATKAALNSFTRSWSKELGKHGIRVVGIAPGILEKTGLRTPEYEEALAWTRNITVEQ
                     LREGYTKNAIPIGRAGRLAEVADFVCYLLSERASYITGVTTNIAGGKTRG"
     gene            complement(12705..13652)
                     /locus_tag="ACHOSC_RS12495"
                     /old_locus_tag="pgaptmp_002499"
     CDS             complement(12705..13652)
                     /locus_tag="ACHOSC_RS12495"
                     /old_locus_tag="pgaptmp_002499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709812.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0030246 - carbohydrate binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar-binding transcriptional regulator"
                     /protein_id="HGU4548424.1"
                     /translation="MENSDDIRLIVKIAQLYYEQDMTQAQIARELRIYRTTISRLLKR
                     GRDQGIVTIAINYDYNENLWLEQQLKQKFGLKDVVVVSGNDEDEETQLAMMGLHGAQL
                     LDRLLEPGDIVGFSWGRAVSALVENLPQAGQSRQLICVPIIGGPSGKLESRYHVNTLT
                     YSAAAKLKGESHLADFPALLDNPLIRNGIMQSQHFKTISAYWDNLDVALVGIGSPAIR
                     DGANWHAFYGGEESDDLNARQVAGDICSRFFDIHGAMVETNMSEKTLSIEMNKLKQAR
                     YSIGIAMSEEKYSGIIGALRGKYINCLVTNSSTAELLLK"
     gene            14000..14872
                     /gene="rluF"
                     /locus_tag="ACHOSC_RS12500"
                     /old_locus_tag="pgaptmp_002500"
     CDS             14000..14872
                     /gene="rluF"
                     /locus_tag="ACHOSC_RS12500"
                     /old_locus_tag="pgaptmp_002500"
                     /EC_number="5.4.99.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA pseudouridine(2604) synthase RluF"
                     /protein_id="HGU4548425.1"
                     /translation="MLPDSSVRLNKYISESGICSRREADRYIEQGNVFLNGKRATIGD
                     QVKPGDIVKVNGQLIEPREAEDLVLIALNKPVGIVSTTEDGERDNIVDFVNHSKRVFP
                     IGRLDKDSQGLIFLTNHGDLVNKILRAGNDHEKEYLVTVDKPITDEFIRGMGAGVPIL
                     GTVTKKCKVKKEAPFVFRITLVQGLNRQIRRMCEHFGYEVKKLERTRIMNVSLSGIPL
                     GEWRDLTDDELIDLFKLIENSSSEVKPKAKAKPKTAGIKRPVVKMEKTAEKGGRPASN
                     GKRFTSPGRKKKGR"
     gene            complement(14873..15145)
                     /locus_tag="ACHOSC_RS12505"
                     /old_locus_tag="pgaptmp_002501"
     CDS             complement(14873..15145)
                     /locus_tag="ACHOSC_RS12505"
                     /old_locus_tag="pgaptmp_002501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3811 domain-containing protein"
                     /protein_id="HGU4548426.1"
                     /translation="MALPRITQKEMTEREQRELKTLLDRARIAHGRVLTNSETNSIKK
                     EYIDKLMVEREAEAKKARQLKKKQAYKPDPEASFSWSANTSTRGRR"
BASE COUNT         3607 a         3986 c         4009 g         3610 t
ORIGIN      
        1 aatctcataa tgcagccgta aaagtttcgg tggaatgaga tcttgcgatt ttgttaatga
       61 ttaagtagtt tgatgtttaa aaataatgct gagttaataa aagtgtgtga agtgtatgac
      121 attaagttat tttggcgtca atgcgattaa cagacaccct tattctattg ccactcaggt
      181 atgatgggca gaatattgcc tctgcccgcc agaaaaagtc agcgcattcc accgtacgcc
      241 agcgtcactt ccttcgccgc tttaatcacc atcgcgccaa actcggtcac gcggtcatcg
      301 gtaatacgtg aaatcggtcc ggaaattgaa attgcggcaa acggttcgcg gtgctcatcg
      361 aaaatacacg ctgcaaggca acgtagcccc agtgcatgtt cctcatcgtc aaatgaataa
      421 ccccgtttgc gcgtttgggc gagatcttct tttaaatgca caggagacac cagcgttgcg
      481 tgggtatagg catgtaaccc tttgcggtgc agcagcttcg tcacctgttc ttcgctcagt
      541 tgggctaaaa aggctttacc cgcaccggaa gcgtgcatcg gcaatttacc gccgataggc
      601 gcggacattc gcatcagatg cgtacactgt acctggtcga taataatcgc ttcgtgatcg
      661 ctttgatcaa gcaccgccat attgaccgtt tcgccagact cttccattaa attgcgcagg
      721 atagggtgaa caatcgctaa caaattacgg ctctggagaa agctgctgcc gaccataaag
      781 gcatgtgcgc cgattgccca atgtcccagt tcgccgacct gacgcacgaa accctgctgt
      841 tgcatcgtgg ttagcaggcg gtgggtcgtg gaattgggta acccggcttg ttgcgccagc
      901 tctgtgagtg ccacactgcc attggattcg gcaatccact ccagtaattt caggccacgc
      961 gttaaagact gaacctgtcc agtcgctggt gcggtggcaa cggcgggttt tctgccgcgt
     1021 ttcgcgggaa tgggtgcgac catgacagcc tcctttttct gtatcgtgga aatcattttc
     1081 atttttattg ttagctaatg caatagttgc tgaactgatc cgatgagtta atgttgaaca
     1141 aatctcatgt tgcgtggtgg tcgcttttac cacagatgcg tttatgccag tatggtttgt
     1201 tgaattttta ttaaatctgg gttgagcgtg tcgggagcaa gtgtgagcag caaagtggaa
     1261 caactgcgtg cgcagttaaa tgaacgtatt ctggtgctgg acggcggtat gggcaccatg
     1321 atccagagct atcgactgaa cgaagccgat tttcgtggtg aacgctttgc cgactggcca
     1381 tgcgatctca aaggcaacaa cgacctgctg gtgctcagca aaccggaagt gatcgccgct
     1441 atccacaacg cctactttga agcgggcgcg gatatcatcg aaaccaacac cttcaactcc
     1501 acgaccattg cgatggcgga ttaccagatg gaatccctgt cggcggaaat caactttgcg
     1561 gcggcgaaac tggcgcgcgc ctgtgctgac gagtggaccg cgcgcacgcc ggagaaaccg
     1621 cgctacgttg ccggtgttct cggcccaacc aaccgcacga cgtctatttc tccggacgtc
     1681 aacgatccgg cattccgcaa catcactttt gaccagctgg tggcggccta tcgcgaatcc
     1741 accaaagcgc tggtggaagg tggtgcggat ctgatcctga ttgaaaccgt tttcgacacc
     1801 cttaacgcca aagcggcggt ttttgcggtg aaaacggagt ttgaagcgct gggcgttgag
     1861 ctgccgatta tgatctccgg caccatcacc gacgcctccg ggcgcacgct ctccgggcag
     1921 accaccgaag cattttacaa ctcattgcgc cacgccgaag ctctgacctt tggcctgaac
     1981 tgtgcgctgg ggcccgatga actgcgccag tacgtgcagg agctgtcacg gattgcggaa
     2041 tgctacgtca ccgcgcaccc gaacgccggg ctacccaacg cctttggtga gtacgatctc
     2101 gacgctgaca cgatggcaaa acagatacgt gaatgggcgc aagcgggttt tctcaatatc
     2161 gtcggcggct gctgtggcac cacgccacaa catattgcag cgatgagtcg tgcagtagaa
     2221 ggattagcgc cgcgcaaact gccggaaatc cccgtagcct gccgtttgtc cggcctggag
     2281 ccgctgaaca ttggcgaaga tagcctgttt gtgaacgtgg gtgaacgcac caacgtcacc
     2341 ggttccgcta agttcaagcg cctgatcaaa gaagagaaat acagcgaggc actggatgtg
     2401 gcccgccagc aggtggaaaa cggtgcgcag attatcgata tcaacatgga tgaagggatg
     2461 ctcgacgccg aagcggcgat ggtgcgtttt ctcaatctga ttgccggtga accggatatc
     2521 gctcgcgtgc cgattatgat cgactcctca aaatgggatg tcattgaaaa aggtctgaag
     2581 tgtatccagg gcaaaggcat tgttaactct atctcgatga aagagggcgt cgacgccttt
     2641 atccatcacg cgaagctctt gcgtcgttac ggtgcggcag tggtggtaat ggcctttgac
     2701 gaacagggac aggccgatac ccgcgcacgg aaaatcgaga tttgccgtcg ggcgtacaaa
     2761 atcctcaccg aagaggttgg cttcccgcca gaagatatca tcttcgaccc gaacatcttc
     2821 gcggttgcca ccggcattga agagcacaac aactacgcgc aggactttat cggcgcgtgt
     2881 gaagacatca aacgcgaact gccgcacgcg ctaatttccg gcggcgtatc taacgtttct
     2941 ttctcgttcc gtggcaacga tccggtgcgc gaagccattc acgcagtgtt cctctactac
     3001 gctattcgca atggcatgga tatggggatc gtcaatgccg ggcagctggc gatttacgac
     3061 gacctgcccg ctgaactgcg cgacgcggtg gaagatgtga ttcttaatcg tcgcgatgat
     3121 ggcactgagc gtttactgga gcttgccgag aaatatcgcg gcagcaaaac cgacgacacc
     3181 gctaacgccc agcaggcgga gtggcgctcg tgggaagtga ataaacgtct ggaatactcg
     3241 ctggtcaaag gcattaccga gtttatcgag caggataccg aagaagcccg ccagcaggct
     3301 acgcgcccga ttgaagtgat tgaaggcccg ttgatggacg gcatgaacgt ggtcggcgat
     3361 ctgtttggcg aagggaaaat gttcctgcca caggtggtca aatcggcgcg cgtcatgaaa
     3421 caggcggtgg cgtatctcga accgtttatt gaagccagca aagagcaggg caaaaccaac
     3481 ggcaagatgg tgatcgccac cgtgaagggc gacgtccacg acatcggtaa aaatatcgtt
     3541 ggtgtggtgc tgcaatgtaa caactacgaa attgtcgatc tcggcgttat ggtgcctgcg
     3601 gaaaaaattc tccgtaccgc taaagaagtg aatgctgatc tgattggact ttcggggctt
     3661 atcacgccgt cgctggacga gatggttaac gtggcgaaag agatggagcg tcagggcttc
     3721 accatcccgc tattgattgg aggcgcgacg acctcaaaag cgcacacggc ggtgaaaatt
     3781 gagcagaact acagcggccc gacggtgtat gtacagaacg catcgcgcac cgttggtgtg
     3841 gtggcggcgc tgctttccga tactcagcgt gatgattttg tcgctcgtac ccgcaaggag
     3901 tacgaaaccg tacgtattca gcacgggcgt aagaaaccgc gcacaccacc ggtcacgctg
     3961 gaagcggcgc gcgataacga tttcgctttt gactggcagg cttacacgcc gccggtggcg
     4021 caccgtctcg gcgtgcagga agttgaagcc agcatcgaaa ctctgcgtaa ttacatcgac
     4081 tggacaccgt tctttatgac ctggtcgctg gccgggaagt atccgcgcat tctggaagat
     4141 gaagtggtgg gcgttgaggc gcagcggctg tttaaagacg ccaacgacat gctggataaa
     4201 ttaagcgccg agaaaacgct gaatccgcgt ggcgtggtgg gcctgttccc ggcaaaccgt
     4261 gtgggcgatg acattgaaat ctaccgtgac gaaacgcgca cccatgtgat caacgtcagc
     4321 caccatctgc gtcaacagac cgaaaaaacg ggattcgcta actactgtct cgctgacttc
     4381 gttgcgccga agctttctgg taaagcagat tacatcggcg catttgccgt gactggcggg
     4441 ctggaagagg acgcactggc tgatgccttt gaagcgcagc acgatgatta caacaaaatc
     4501 atggtgaaag cgcttgccga ccgtttagcc gaagcctttg cggagtatct ccatgagcgt
     4561 gtgcgtaaag tctactgggg ctatgcgccg aacgagaacc tcagcaacga agaactgatc
     4621 cgcgaaaact accagggcat ccgtccggca ccgggctacc cggcctgccc ggaacatacg
     4681 gaaaaagcca ccatctggga gctgctggaa gtggaaaaac ataccggcat gaaactcaca
     4741 gaatctttcg ccatgtggcc cggtgcatcg gtttcgggtt ggtatttcag ccacccggac
     4801 agcaagtact acgcggtggc gcaaattcag cgcgatcagg ttgaagacta tgcccgccgt
     4861 aaaggtatga gcgtctccga tgttgagcgc tggctggcac cgaatctggg gtatgacgcg
     4921 gactgattca caaatctgtc acttttcctt acaacaaaca gggcgctcaa tgagtgccct
     4981 gtctctttat taatatgaaa cacttatact ggaaacaggc tggaataatc ttagccggaa
     5041 aaacgaatgt caaaaggcac agtaaagatg ccagtcagcg tgtttccggt cctttggtca
     5101 ttcaattgct tcaggaacat ataacgataa ggagaacctg aactcgtgtt aacgctgctt
     5161 cacctgcttt ctgccgtcgc cctgctggtc tgggggactc atattgttcg aaccggcgta
     5221 atgcgcgtct tcggcgcgcg tttgcgtact gtccttagcc ggagcgtcga aaaaaagccg
     5281 ctcgcctttt gcgcggggat cggcgttacc gcactggtgc agagcagtaa tgccaccacc
     5341 atgctggtga cctcgtttgt cgctcaggat ctggtagccc tcgcaccggc tctggtcatt
     5401 gtgctggggg cagatgtcgg gacggcgcta atggcgcgta ttctcacctt cgacttatcc
     5461 tggctgtcac cgttacttat ttttatcggc gtgatttttt tcctcggacg caaacagtca
     5521 cgcgccgggc aactgggccg cgtcggtatt ggtcttgggc tgattttact ggcgctggag
     5581 ttgattgtgc aggccgtaac gccgatcacc caggcaaacg gcgttcaggt gatctttgcc
     5641 tcgctgaccg gcgatattct gctggatgcg ctgattggcg cgatgttcgc cattatcagc
     5701 tactccagcc ttgctgctgt actgctgact gcgactctga ccgccgcagg cattatctcc
     5761 ttccccgtgg cgctctgtct ggtgattggt gctaacctcg gttccggcct gctggcgatg
     5821 ctcaacaaca gtgccgccaa tgccgcagcc cgccgtgtcg cgctgggtag tctgctgttt
     5881 aagctggtgg gtagcctgat tatcctgccg tttgtccatt tgctggcaga gacaatgggg
     5941 aagctgtcat tgccaaaagc ggaactggtg atctatttcc acgtcttcta caaccttgta
     6001 cgttgcctgg tcatgctgcc atttgttgac ccgatggcac ggttttgcaa aacgattatt
     6061 cgcgatgaac cggaactgga tacccagcta cggcctaaac atctggatgt cagcgcgctg
     6121 gatacgccca cgcttgctct ggcgaacgcc gcgcgcgaaa ccctgcgcat tggcgacgcc
     6181 atggaacaga tgatggaagg gctgaataaa gtgatgcacg gcgagccacg gcaggagaaa
     6241 gagctgcgta agctggcaga tgatatcaac gttctctata ccgccattaa gctgtatctg
     6301 gcgcggatgc caaaagagga actggcagaa gaagagtcgc gtcgctgggc ggagattatt
     6361 gaaatgtcgc tcaaccttga acaggcttcc gatatcgtcg agcgcatggg cagcgaaatt
     6421 gccgataaat cgctggcagc acggcgggca ttttcgcttg atgggttgaa ggaactggat
     6481 gcgctctatg agcaattgct cagtaattta aagctggcaa tgtcggtgtt cttctctggc
     6541 gatgtcacca gcgctcgtcg tttgcgtcgc agcaaacatc gttttcgcat tcttaatcgc
     6601 cgctattccc acgcccacgt cgatcgcctg catcagcaaa acgtgcagag cattgaaacc
     6661 agttcgctac atttaggctt actgggagat atgcagcgcc tgaactcgct gttttgttcg
     6721 gtggcttaca gtgtgctgga acagccggat gaagatgaag gacgggacga gtattaatat
     6781 tgtgaatcct gtaggcctga taagacgcgt caagcgtcgc atcaggcact atgaacaaga
     6841 tgccggatca tgcatccggc aatttactta aaaacggtga cctgcttcta gcggcaccgc
     6901 ttcttcacca gccttaaaca cataagtggt gttcgggcca cccagcgtag cgtgaccttt
     6961 actcactgtg atccagttac cttccggtag accaataatc gtcagttctg gcgcgacgac
     7021 cagcagttcg cgaatacgct gctcacgggt ttcaccttta tggccttccg gcagcgcgtt
     7081 ggtgaagtgc gggttgattt gcagcgggaa cagatttagc gcatcgaaac cttgcggatc
     7141 gacaatcggc atatcgttgg tggtacgaat ggttgggcaa gcaaggttag cgcctgcgct
     7201 ccagccaata tacagcgcac cacgtttaac cacgtcagta attggtgcca gcagcccgcg
     7261 ctcgcggcac tgtttcagca actggaaagt attcccgccc ccgacaatca cgatctcagc
     7321 attttcaatc gcggcaacgg gatcgacaac gctatgaata ccggtgacag atacacccag
     7381 cggagcgaga accgcagccg ttttcgctgt gtaatcatcc caggtctgcg ttacgccagc
     7441 gaaaggaata aataccgctg aacggcgacc ctgcaactgt tcagcaatca gcggcagcgc
     7501 atgttccagc caggctttac ccggcagcgt cgagttactc aataaaagca gttccattac
     7561 ttctccagtt agtaagaaat cacgggaggc atgctaccac tgcgaattta accacttact
     7621 gacatggctc acgatgtcac gtattgcacc gggcgctgag actgatgaca aacacagcat
     7681 tgcctgatgc gctacgctta ccaggcctac cagataactg caatatattg aattttcgag
     7741 attatgtagg ccgaataagg ctttattccg catccggcat aaacaatgtg tactttgcca
     7801 ccaatttgag cgccccttaa ggcgctgatg tttttttatc cttctcgtgc acattgggcc
     7861 gcttcagcaa tcagccacac atcaggtcgc gaagttgccg cggcgaccag ccagcgacat
     7921 gcagagttaa gtagcgcgat taatcatggg aaattgcctc tgcatgagtg agcagatatt
     7981 gctccgcctc accggaccag atcccctgat gacgcgccag aatcgccgcc agtgcttgat
     8041 cctgaatctg cgtgagtgac gtcagcggca ggtatttccc ggtatacacc agctttttgc
     8101 cgccgccgac ggcaggcaat tcaagcgtgg tttcgcccgc ggcattcagc ccaagaatat
     8161 gtgttaccac ttttgcggcc tgcacttttt tctcttcaat cagtttgacc gccgcgcgca
     8221 tgtcgtcggt attgccgcct gacgtgccca cgtagtgggt aaatgcataa tgcacatcgt
     8281 agaaattaat tggcgcgctg aaatgtttat cctgcggtcc ggcgaagaaa ttcaggcaac
     8341 catctgtcgc cagcaaggaa gaggcgagag tcaccagtcc ttcattaggc acaaagacga
     8401 aaatatcatc gaagccgtga ccgccactca gcgccattag cgtatcaaat gctgcatcgg
     8461 cggcattgag ataatgaatc agtgtttgcg gttctgatgg atagtgcttg cgcgcataac
     8521 tcaatttatc gttgtcggta tcggtaatga cgagcagcga cgggttaacg ggtccatgta
     8581 gcgcataatc aatcgccaac agtcccattg gtccggtgcc gccgaggatc agcatgcgcc
     8641 cttgcgggcg aatccccatc gtgtggttat aactaccttc ctgaagatga tagttggcgt
     8701 tgaacgcgcc aatcacgcag gaaagcggtt caaccagtga gccttcaaaa taggtttcgc
     8761 cgtcgtatgc cagcaggcaa tcttgttcca tgacctcgtt gggaataacc acatgcgtgg
     8821 cttcgccgcc cacccacggg aaggagtagc cggggcagtc cgggcggtcg gggagttgca
     8881 ggttggcctg aatcacataa cgctgacccg gctggaattt gtgctgccac tttttaccca
     8941 cggccagaat atcgccgcaa aactcgtggc cgatgatgat ggggttggtc gccacatcgt
     9001 cgggtacttt tttatggttt tcacccagat tggcctcttt ccaggaagag aggcacaggc
     9061 tgtcagtgac caccgtcgcg aggatttcat cctcctgcat ttcaggaagg tcaaaggttt
     9121 ccaggcgtaa atcacgttta ccataaagac gcagagctgt tgttttcata aaccacctca
     9181 tgttgagcga tggccccggc agtcgtgccg gggcgaagaa tcaggacagg aagcccagcg
     9241 cattaccgat aatccctaag ccaaacaggg cgaaaatcag ccataccggg ttaatttttt
     9301 tgttgagcag acgaaccata agcagcgtca gaccgagcgc cagcaaacca gggcaaagtt
     9361 ggtcgagaat gttctgcacg gtcatggtga cggtggagcc atcggcggca tgcgtttgtg
     9421 aaaccaccaa cggtacgttg attgacgtcc atttggtgac cagcacgccc atcacaaaca
     9481 gtccgagaat cgacgcgcct tcggtgagtt tttgcagcac attcccgccc atatcgctga
     9541 cgatattcac ccctttgcga aagccgagct gtaggccata ccacttcatc gccagacgca
     9601 ccgcattgaa aataaagaaa aaaaaagcgg gccgaggatg ttgccagaaa gtgccagaga
     9661 tgcgccgagc gcagcggtaa tcgggcgcag cgttccccaa accagtggat cgccaacccc
     9721 tgccaatggt cccatcagac cgactttgat gccgttaatg gtaccgtcat caatttccgc
     9781 gccgttggcc cgcgcctctt ccatggcggc agtgacgccg atgaccgggc cacatacggc
     9841 tggcgtggta ttgaagaaca ccaggtgtcg cctgagcgcc gcaacctgat cctcttttaa
     9901 tgggtaaagt cgcttgatgg cggggatcat gtcgtagcaa aagcccagcc cgtgaatacg
     9961 ttcaaagtta aaggacgcct gttgcaggtt ggagcgcaga aacatgctca ccagatcgct
    10021 gcgtgtaatt tttctctgtt ccatgatggc tccgttaatc gtcaagttgg tcaagggcgg
    10081 tggaggtggt ggcctggggt tgaggttcag ctttacgcca ctgtggattt agctggatgt
    10141 agaccagggc catgatcacg ccgacgccac cgaacgccag cagactgaga tcgaggtagc
    10201 cacctgcgag gaaaccgagg aaaaagaaag gcatcaaata tttcacgccc atcatacgta
    10261 gcaccatggc gtaaccgacc accacgataa aaccgccagc aatttgcaga ccgcgcgtca
    10321 cgaattccgg tatcgcgctc agcatattgc tcaccatatc ggcgctgacg aacagtgaga
    10381 caatcagtgc cggaatagca acgcgcagcg cctgcacgcc aagcgcggag acatgcagaa
    10441 tatcgagagt gcgaaaccgc gcttcttccg ctgctttatc cgccgcgtgc tggaacacta
    10501 cggtgatggt acgggcaaac acagtcagca cctggcctgc cgcagccaca ggcaacgcga
    10561 tggcgattcc ggtggcgatg ctttgctgac caacgataac cagaatggcg gagattatgc
    10621 tggcgagtgc agaatccgga gattgcgccg cgccgacgtt catccagccg agagctatca
    10681 attccagcgt accaccgagt attattccgg tttttaaatc accgagaatt aaaccaatca
    10741 ccgtacaggc aattaacggg cgatgagtct gaaattcatc cagcacgctg cccattccgg
    10801 caatacagga aaaaagaaat atagcaatga tttgtagggt acttatttcc atgatttttc
    10861 accttttaag tgtcgtcatc cctgcctgtt aaagcatatg tacacaggcg ctattattta
    10921 atttgcgatt aactgttcgt ttattttgtc gataatattg attgagggat ctgacgctac
    10981 gacgcgtaaa tccagtatga cgccaagatt atttaactca tgaaatgcgt taatatcatc
    11041 atcatctaat gaaaccgctt tggttaactg ttttttaccg ggccgccagg ccatgccgcc
    11101 aatattcagg gtgtctattt ttaccccctg gcgaaccatc gctaaggcat cctgtgggcg
    11161 agtgaaaaga taaaaaacgg tttcgtcctg atattgcggg ttgtgataaa cagcgacggc
    11221 tttttcaata ttgaccacgt taaccttcat tcccggtggc gcagcctgac gcaataatgt
    11281 ccggcgaact tcatcgttat aaacttcgtc attgcagata ataatgcgct gggcgttagc
    11341 cactttcgac cagacggtag tgacctgacc gtggatcagg cggtcatcaa tgcgggcgag
    11401 cgtaatgttc atcagaaatc ctcctctgcc gttgccagtt gtttccagac aacgcaggtt
    11461 tgctttgcga tttgcgtcag gtgttcacac agttcatcaa cattcatgct gtcctggtta
    11521 tcaaccagtt cgagcgccag cggcagagaa aggccgctga ttacccgcag acgaggattg
    11581 cgcatcgcaa gcatcgctgc cgcattccac gggctgccgc actgtaaatc cacggcaatc
    11641 agccactcat cttgattgtg aatgcttact aacttttcca gctttgctac gatgtctccg
    11701 gcgttctcgc cgggcacaaa ttcaacggct tcaacattgg cgttgccgta aaccatctga
    11761 accgattcca gcatggcgca agctaatttg ccgtgggcac aaaaaatggc gttgaccata
    11821 aagcctcctt agccgcgcgt tttgccgccc gcaatgttag tggttactcc ggtgatgtag
    11881 ctggcgcgtt cagacaacag ataacaaaca aaatcagcga cttctgctaa tcttccggcg
    11941 cgcccaatag gaatggcgtt tttggtatag ccttcacgca gctgctcgac ggtgatattg
    12001 cgcgtccacg ccagcgcctc ttcatattcc ggcgtgcgta gcccggtttt ttccagaatc
    12061 ccgggcgcga taccgaccac acggataccg tgtttaccca gctctttcga ccaggagcgc
    12121 gtgaagctat taagcgcggc tttggtagcg gcgtaacagc tttggccttc tgagccttcc
    12181 agtccacttt ctgaggaaac attcacaatc acgccatcat gttgtttgac catctgtcgc
    12241 gccaccgcct gcgacatcag aaaaacgcct ttctggttga tattgaccat tttttcgaat
    12301 gcagcttcgt tgagttcata ctgcccggca ggcgctttct catcgaccag cagacgcggg
    12361 aaattgaccc cggcgttatt gaccagaccg tcgatgcgac caaaacgctg gataatttcc
    12421 gctaccgtat gatttacctc tttggcgctg gaaatatcgg tcggccaaaa ctgatagcct
    12481 ttatgacctt catattgacc atcgccaccg tgaatatcta ccatctgtac gtttgcgcct
    12541 tgtgctaata attcctctac aatcgccaga ccaataccgg atgcgccgcc ggtgacgata
    12601 atgattttat cctgcaaatt taaccacgtt tgcataacag actcctgatt ttagataata
    12661 gggacccgcc gtaattttta ttacggtaaa catcgggaag atttttattt cagtaatagt
    12721 tcagctgtgc tgctattcgt taccagacaa ttaatatatt ttccacgcag tgcaccaata
    12781 attccgctgt atttttcttc actcatggca atgccaatgg aatatcgcgc ctgctttaat
    12841 ttattcattt cgatagagag cgttttttcg ctcatattcg tttcaaccat tgcgccgtga
    12901 atatcaaaaa agcgcgagca aatatcgcca gcaacctggc gggcattcag gtcgtcactc
    12961 tcttcaccac cataaaacgc atgccagtta gcgccgtcgc gaatggccgg tgagccaatt
    13021 cccaccaggg cgacatccag attatcccag taggcagaga tggttttaaa gtgctgagac
    13081 tgcatgatcc cattacgaat taatgggtta tccagcagag ccggaaaatc cgcgagatgc
    13141 gattcccctt tcagcttcgc tgccgcgctg taggttaatg tgtttacatg atagcggctt
    13201 tcgagtttac cggacggtcc gccaataatt ggtacgcaga ttaactgccg cgattgcccc
    13261 gcctgcggca agttttcaac cagtgcgctc accgcgcgac cccaggaaaa accgacaata
    13321 tcgccaggct ccagcaagcg atccagcagt tgcgcgccgt gtaaccccat catcgccagt
    13381 tgagtctctt catcctcatc atttcccgac accaccacaa cgtctttcag gccaaacttt
    13441 tgcttcagtt gctgctccag ccagagattt tcgttgtagt catagttgat ggcgatggtg
    13501 acaattccct gatcgcggcc tcgtttaagc aagcggctga tggtggtgcg gtaaatacgc
    13561 agttcgcgcg cgatttgcgc ctgcgtcata tcctgttcgt aatagagttg ggcaatcttc
    13621 acaatcaaac ggatatcgtc actgttttcc attgccgcct cctggttctg cacatttgtg
    13681 caaatacgct ttctcaccgc ttgatctgat ataaccccat catgagggcc tttcaaagcg
    13741 atttttcact ctttgcatgg ggagaaatag cgctttctct tgcacatttg tttctctgcg
    13801 tagtagtcat cagcacaggt gtgcagcgat tcgtaatatg ttgattatcc ttattttgcc
    13861 aggcaaattt gcatcgtact tccggagaca cttttgtgat aaaaggcgtg gcagatcaca
    13921 tttaatttcg acgttgccgc ataaccctgt ttttccccgc taagaggtac ggtatattcc
    13981 gcgttttaca ggagaattta tgctgcccga ctcatcagtc cgtttaaata aatacatcag
    14041 cgaaagcgga atttgctcac gccgcgaagc ggatcgctat atcgagcaag gcaatgtgtt
    14101 ccttaatggc aagcgagcca ccattggcga tcaggtgaaa cccggcgaca ttgtgaaagt
    14161 aaacggtcag ttgattgagc ctcgggaagc cgaagatttg gtacttatcg ccctgaacaa
    14221 gcccgtcggt attgtcagca ccaccgaaga tggcgagcgc gataacattg tcgatttcgt
    14281 taaccacagc aaacgcgtgt tcccgattgg ccgcctggat aaagactccc aggggctgat
    14341 tttcctcacc aatcacggcg atctggtgaa taagatcctc cgtgcgggta acgatcatga
    14401 gaaagagtat ctggtgacgg tcgataaacc gattaccgat gaatttattc gtggtatggg
    14461 ggcgggggtg ccgatcctcg ggacggtgac gaaaaagtgc aaagtcaaaa aagaagcgcc
    14521 gtttgtcttc cgcattaccc tggtgcaggg gctgaaccgt cagatccggc gcatgtgcga
    14581 gcatttcggc tatgaagtga aaaagctgga acgtacgcgc attatgaacg tcagtttaag
    14641 cggcattccg ctgggtgagt ggcgcgattt aactgacgat gagttaatcg acctgtttaa
    14701 actcattgaa aattcctctt ccgaggtaaa acctaaagcg aaggctaaac cgaaaacagc
    14761 gggcatcaaa cgtccagtcg ttaagatgga aaaaacggcg gaaaaaggcg gtcgcccggc
    14821 gtccaacggt aagcgtttta cctcgccggg gcgtaaaaag aagggacgct aatcagcgcc
    14881 tgccgcgcgt cgacgtattt gctgaccagc taaatgacgc ttcaggatcc ggtttataag
    14941 cctgcttttt cttcaactgg cgggcttttt tcgcttcagc ctcacgctcg accattaatt
    15001 tatcgatgta ctctttctta atgctgttgg tttcggagtt ggtcagtacg cgaccatgcg
    15061 caatccgcgc ccgatccagc agtgttttaa gttcacgctg ctcgcgttca gtcatctctt
    15121 tttgggtaat acgggggagt gccatgatgg tgccctcagt gagccagaga gttcagtgta
    15181 cgggaattcg tttgcgagct gaactgataa ta
//
LOCUS       DAZKHF020000099        15043 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2201S31241
            SAMN43267800-rid25106413.denovo.100, whole genome shotgun sequence.
ACCESSION   DAZKHF020000099 DAZKHF020000000
VERSION     DAZKHF020000099.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267800
            Sequence Read Archive: SRR30307263
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 15043)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15043)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 15043)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350581.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350581.2
            Genome Coverage       :: 151x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:21:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,652
            CDSs (total)                      :: 5,557
            Genes (coding)                    :: 5,320
            CDSs (with protein)               :: 5,320
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 237
            CDSs (without protein)            :: 237
            Pseudo Genes (ambiguous residues) :: 0 of 237
            Pseudo Genes (frameshifted)       :: 102 of 237
            Pseudo Genes (incomplete)         :: 130 of 237
            Pseudo Genes (internal stop)      :: 52 of 237
            Pseudo Genes (multiple problems)  :: 41 of 237
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15043
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267800-rid25106413.denovo.100"
                     /serotype="O 26:H11"
                     /isolate="2201S31241"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-01"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            4..981
                     /gene="ybeQ"
                     /locus_tag="ACHOSC_RS12510"
                     /old_locus_tag="pgaptmp_002502"
     CDS             4..981
                     /gene="ybeQ"
                     /locus_tag="ACHOSC_RS12510"
                     /old_locus_tag="pgaptmp_002502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415177.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Sel1 family TPR-like repeat protein YbeQ"
                     /protein_id="HGU4548427.1"
                     /translation="MIFTSSCCDNLSIDEIIERAEKGDCEAQYIVGFYYNRDSAIDSP
                     DDEKAFYWLKLAAEQGHCEAQYSLGQKYTEDKSRHKDNEQAIFWLKKAALQGHTFASN
                     ALGWTLDRGEAPNYKEAVVWYQIAAESGMSYAQNNLGWMYRNGNGVAKDYALAFFWYK
                     QAALQGHSDAQNNLADLYEDGKGVAQNKTLAAFWYLKSAQQGNRHAQFQIAWDYNAGE
                     GVDQDYKQAMYWYLKAAAQESVGAYVNIGYMYKHGQGVEKDYQAAFEWFTKAAECNDA
                     TAWYNLAIMYHYGEGRPVDLRQALDLYRKVQSSGTRDVSQEIRETEDLL"
     gene            complement(1051..1533)
                     /gene="ybeL"
                     /locus_tag="ACHOSC_RS12515"
                     /old_locus_tag="pgaptmp_002503"
     CDS             complement(1051..1533)
                     /gene="ybeL"
                     /locus_tag="ACHOSC_RS12515"
                     /old_locus_tag="pgaptmp_002503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon-containing protein"
                     /protein_id="HGU4548428.1"
                     /translation="MNKVAQYYRELVASLSERLRNGERDIDALVEQARERVIKTGELT
                     RTEVDELTRAVRRDLEEFAMSYEESLKEESDSVFMRVIKESLWQELADITDKTQLEWR
                     EVFQDLNHHGVYHSGEVVGLGNLVCEKCHFHLPIYTPEVLTLCPKCGHDQFQRRPFEP
                     "
     gene            1768..4350
                     /gene="leuS"
                     /locus_tag="ACHOSC_RS12520"
                     /old_locus_tag="pgaptmp_002504"
     CDS             1768..4350
                     /gene="leuS"
                     /locus_tag="ACHOSC_RS12520"
                     /old_locus_tag="pgaptmp_002504"
                     /EC_number="6.1.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008456064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004823 - leucine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006429 - leucyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="leucine--tRNA ligase"
                     /protein_id="HGU4548429.1"
                     /translation="MQEQYRPEEIESKVQLHWDEKRTFEVTEDESKEKYYCLSMLPYP
                     SGRLHMGHVRNYTIGDVIARYQRMLGKNVLQPIGWDAFGLPAEGAAVKNNTAPAPWTY
                     DNIAYMKNQLKMLGFGYDWSRELATCTPEYYRWEQKFFTELYKKGLVYKKTSAVNWCP
                     NDQTVLANEQVIDGCCWRCDTKVERKEIPQWFIKITAYADELLNDLDKLDHWPDTVKT
                     MQRNWIGRSEGVEITFNVNDYDNTLTVYTTRPDTFMGCTYLAVAAGHPLAQKAAENNP
                     ELAAFIDECRNTKVAEAEMATMEKKGVDTGFKAVHPLTGEEIPVWAANFVLMEYGTGA
                     VMAVPGHDQRDYEFASKYGLNIKPVILAADGSEPDLSQQALTEKGVLFNSGEFNGLDH
                     EAAFNAIADKLTAMGVGERKVNYRLRDWGVSRQRYWGAPIPMVTLEDGTVMPTPDDQL
                     PVILPEDVVMDGITSPIKADPEWAKTTVNGMPALRETDTFDTFMESSWYYARYTCPEY
                     KEGMLDSEAANYWLPVDIYIGGIEHAIMHLLYFRFFHKLMRDAGMVNSDEPAKQLLCQ
                     GMVLADAFYYVGENGERNWVSPVDAIVERDEKGRIVKAKDAAGHELVYTGMSKMSKSK
                     NNGIDPQVMVERYGADTVRLFMMFASPADMTLEWQESGVEGANRFLKRVWKLVYEHTA
                     KGDVAALNVDALTEDQKALRRDVHKTIAKVTDDIGRRQTFNTAIAAIMELMNKLAKAP
                     TDGEQDRALMQEALLAVVRMLNPFTPHICFTLWQELKGEGDIDNAPWPVADEKAMVED
                     STLVVVQVNGKVRAKITVPVDATEEQVRERAGQEHLVAKYLDGVTVRKVIYVPGKLLN
                     LVVG"
     gene            4365..4946
                     /gene="lptE"
                     /locus_tag="ACHOSC_RS12525"
                     /old_locus_tag="pgaptmp_002505"
     CDS             4365..4946
                     /gene="lptE"
                     /locus_tag="ACHOSC_RS12525"
                     /old_locus_tag="pgaptmp_002505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA];
                     GO_process: GO:0043165 - Gram-negative-bacterium-type cell
                     outer membrane assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS assembly lipoprotein LptE"
                     /protein_id="HGU4548430.1"
                     /translation="MRYLATLLLSLAVLITAGCGWHLRDTTQVPSTMKVMILDSGDPN
                     GPLSRAVRNQLRLNGVELLDKETTRKDVPSLRLGKVSIAKDTASVFRNGQTAEYQMIM
                     TVNATVLIPGRDIYPISAKVFRSFFDNPQMALAKDNEQDMIVKEMYDRAAEQLIRKLP
                     SIRAADIRSDEEQTSTTTDTPATPARVSTTLGN"
     gene            4946..5977
                     /gene="holA"
                     /locus_tag="ACHOSC_RS12530"
                     /old_locus_tag="pgaptmp_002506"
     CDS             4946..5977
                     /gene="holA"
                     /locus_tag="ACHOSC_RS12530"
                     /old_locus_tag="pgaptmp_002506"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta"
                     /protein_id="HGU4548431.1"
                     /translation="MIRLYPEQLRAQLNEGLRAAYLLLGNDPLLLQESQDAVRQVAAA
                     QGFEEHHTFSIDPNTDWNAIFSLCQAMSLFASRQTLLLLLPENGPNAAINEQLLTLTG
                     LLHDDLLLIVRGNKLSKAQENAAWFTALANRSVQVTCQTPEQAQLPRWVAARAKQLNL
                     ELDDAANQVLCYCYEGNLLALAQALERLSLLWPDGKLTLPRVEQAVNDAAHFTPFHWV
                     DALLMGKSKRALHILQQLRLEGSEPVILLRTLQRELLLLVNLKRQSAHTPLRALFDKH
                     RVWQNRRGMMGEALNRLSQTQLRQAVQLLTRTELTLKQDYGQSVWAELEGLSLLLCHK
                     PLADVFIDG"
     gene            5979..6620
                     /gene="nadD"
                     /locus_tag="ACHOSC_RS12535"
                     /old_locus_tag="pgaptmp_002507"
     CDS             5979..6620
                     /gene="nadD"
                     /locus_tag="ACHOSC_RS12535"
                     /old_locus_tag="pgaptmp_002507"
                     /EC_number="2.7.7.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate-nucleotide adenylyltransferase"
                     /protein_id="HGU4548432.1"
                     /translation="MKSLQALFGGTFDPVHYGHLKPVETLANLIGLTRVTIIPNNVPP
                     HRPQPEANSVQRKHMLELAIADKPLFTLDERELKRNAPSYTAQTLKEWRQEQGPDVPL
                     AFIIGQDSLLTFPTWYEYETILDNAHLIVCRRPGYPLEMAQPQYQQWLEDHLTHNPED
                     LHLQPAGKIYLAETPWFNISATIIRERLQNGESCEDLLPEPVLTYINQQGLYR"
     gene            6644..7255
                     /gene="cobC"
                     /locus_tag="ACHOSC_RS12540"
                     /old_locus_tag="pgaptmp_002508"
     CDS             6644..7255
                     /gene="cobC"
                     /locus_tag="ACHOSC_RS12540"
                     /old_locus_tag="pgaptmp_002508"
                     /EC_number="3.1.3.73"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043755 - alpha-ribazole phosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylcobalamin/alpha-ribazole phosphatase"
                     /protein_id="HGU4548433.1"
                     /translation="MRLWLIRHGETQANIDGLYSGHAPTPLTARGIEQAQNLHTLLDD
                     VSFDLVLCSELERAQHTARRVLSDRQLPVQIIPELNEMFFGDWEMRHHRDLMQEDAEN
                     YSAWCNDWQHAIPTNGEGFQAFSQRVERFIARLSEYQHYQNILIVSHQGVLSLLIARL
                     IGMPAESMWHFRVDQGCWSAIDINQKFATLRVLNSRAIGVENA"
     gene            7515..7832
                     /gene="rsfS"
                     /locus_tag="ACHOSC_RS12545"
                     /old_locus_tag="pgaptmp_002509"
     CDS             7515..7832
                     /gene="rsfS"
                     /locus_tag="ACHOSC_RS12545"
                     /old_locus_tag="pgaptmp_002509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007373852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0017148 - negative regulation of
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome silencing factor"
                     /protein_id="HGU4548434.1"
                     /translation="MQGKALQDFVIDKIDDLKGQDIIALDVQGKSSITDCMIICTGTS
                     SRHVMSIADHVVQESRAAGLLPLGVEGENSADWIVVDLGDVIVHVMQEESRRLYELEK
                     LWS"
     gene            7836..8303
                     /gene="rlmH"
                     /locus_tag="ACHOSC_RS12550"
                     /old_locus_tag="pgaptmp_002510"
     CDS             7836..8303
                     /gene="rlmH"
                     /locus_tag="ACHOSC_RS12550"
                     /old_locus_tag="pgaptmp_002510"
                     /EC_number="2.1.1.177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016245807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (pseudouridine(1915)-N(3))-methyltransferase RlmH"
                     /protein_id="HGU4548435.1"
                     /translation="MKLQLVAVGTKMPDWVQTGFTEYLRRFPKDMPFELIEIPAGKRG
                     KNADIKRILDKEGEQMLAAAGKNRIVTLDIPGKPWDTPQLAAELERWKLDGRDVSLLI
                     GGPEGLSPACKAAAEQSWSLSALTLPHPLVRVLVAESLYRAWSITTNHPYHRE"
     gene            8334..10235
                     /gene="mrdA"
                     /locus_tag="ACHOSC_RS12555"
                     /old_locus_tag="pgaptmp_002511"
     CDS             8334..10235
                     /gene="mrdA"
                     /locus_tag="ACHOSC_RS12555"
                     /old_locus_tag="pgaptmp_002511"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA];
                     GO_function: GO:0009002 - serine-type D-Ala-D-Ala
                     carboxypeptidase activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-transpeptidase MrdA"
                     /protein_id="HGU4548436.1"
                     /translation="MKLQNSFRDYTAESALFVRRALVAFLGILLLTGVLIANLYNLQI
                     VRFTDYQTRSNENRIKLVPIAPSRGIIYDRNGIPLALNRTIYQIEMMPEKVDNVQQTL
                     DALRSVVDLTDDDIAAFRKERARSHRFTSIPVKTNLTEVQVARFAVNQYRFPGVEVKG
                     YKRRYYPYGSALTHVIGYVSKINDKDVERLNNDGKLANYAATHDIGKLGIERYYEDVL
                     HGQTGYEEVEVNNRGRVIRQLKEVPPQAGHDIYLTLDLKLQQYIETLLAGSRAAVVVT
                     DPRTGGVLALVSTPSYDPNLFVDGISSKDYSALLNDPNTPLVNRATQGVYPPASTVKP
                     YVAVSALSAGVITRNTTLFDPGWWQLPGSEKRYRDWKKWGHGRLNVTRSLEESADTFF
                     YQVAYDMGIDRLSEWMGKFGYGHYTGIDLAEERSGNMPTREWKQKRFKKPWYQGDTIP
                     VGIGQGYWTATPIQMSKALMILINDGIVKVPHLLMSTAEDGKQVPWVQPHEPPVGDIH
                     SGYWELAKDGMYGVANRPNGTAHKYFASAPYKIAAKSGTAQVFGLKANETYNAHKIAE
                     RLRDHKLMTAFAPYNNPQVAVAMILENGGAGPAVGTLMRQILDHIMLGDNNTDLPAEN
                     PAVAAAEDH"
     gene            10238..11350
                     /gene="mrdB"
                     /locus_tag="ACHOSC_RS12560"
                     /old_locus_tag="pgaptmp_002512"
                     /gene_synonym="rodA"
     CDS             10238..11350
                     /gene="mrdB"
                     /locus_tag="ACHOSC_RS12560"
                     /old_locus_tag="pgaptmp_002512"
                     /gene_synonym="rodA"
                     /EC_number="2.4.99.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004885876.1"
                     /note="rod shape-determining protein RodA; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan glycosyltransferase MrdB"
                     /protein_id="HGU4548437.1"
                     /translation="MTDNPNKKTFWDKVHLDPTMLLILLALLVYSALVIWSASGQDIG
                     MMERKIGQIAMGLVIMVVMAQIPPRVYEGWAPYLYIICIILLVAVDAFGAISKGAQRW
                     LDLGIVRFQPSEIAKIAVPLMVARFINRDVCPPSLKNTGIALVLIFMPTLLVAAQPDL
                     GTSILVALSGLFVLFLSGLSWRLIGVAVVLVAAFIPILWFFLMHDYQRQRVMMLLDPE
                     SDPLGAGYHIIQSKIAIGSGGLRGKGWLHGTQSQLEFLPERHTDFIFAVLAEELGLVG
                     ILILLALYILLIMRGLWIAARAQTTFGRVMAGGLMLILFVYVFVNIGMVSGILPVVGV
                     PLPLVSYGGSALIVLMAGFGIVMSIHTHRKMLSKSV"
     gene            11361..12449
                     /gene="rlpA"
                     /locus_tag="ACHOSC_RS12565"
                     /old_locus_tag="pgaptmp_002513"
     CDS             11361..12449
                     /gene="rlpA"
                     /locus_tag="ACHOSC_RS12565"
                     /old_locus_tag="pgaptmp_002513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endolytic peptidoglycan transglycosylase RlpA"
                     /protein_id="HGU4548438.1"
                     /translation="MRKQWLGICIAAGMLAACTSDDGQQQTVSVPQPAVCNGPIVEIS
                     GADPRFEPLNATANQDYQRDGKSYKIVQDPSRFSQAGLAAIYDAEPGSNLTASGEAFD
                     PTQLTAAHPTLPIPSYARITNQANGRMIVVRINDRGPYGNDRVISLSRAAADRLNTSN
                     NTKVRIDPIIVAQDGSLSGPGMACTTVAKQTYALPAPPDLSGGAGASSVSGPQGDILP
                     VSNSTLKSEDPTGAPVTSSGFLGAPTTLAPGVLESSEPTPAPQPVVTAPSTTPATSPA
                     MVTPQAASQSASGNFMVQVGAVSDQARAQQYQQQLGQKFGVPGRVTQNGAVWRIQLGP
                     FASKAEASTLQQRLQTEAQLQSFITTAQ"
     gene            12589..13800
                     /gene="dacA"
                     /locus_tag="ACHOSC_RS12570"
                     /old_locus_tag="pgaptmp_002514"
     CDS             12589..13800
                     /gene="dacA"
                     /locus_tag="ACHOSC_RS12570"
                     /old_locus_tag="pgaptmp_002514"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009002 - serine-type D-Ala-D-Ala
                     carboxypeptidase activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine carboxypeptidase DacA"
                     /protein_id="HGU4548439.1"
                     /translation="MNTIFSARIMKRLALTTALCTAFISAAHADDLNIKTMIPGVPQI
                     DAESYILIDYNSGKVLAEQNADVRRDPASLTKMMTSYVIGQAMKAGKFKETDLVTIGN
                     DAWATGNPVFKGSSLMFLKPGMQVPVSQLIRGINLQSGNDACVAMADFAAGSQDAFVG
                     LMNSYVNALGLKNTHFQTVHGLDADGQYSSARDMALIGQALIRDVPNEYSIYKEKEFT
                     FNGIRQLNRNGLLWDNSLNVDGIKTGHTDKAGYNLVASATEGQMRLISAVMGGRTFKG
                     REAESKKLLTWGFRFFETVNPLKVGKEFASEPVWFGDSDRASLGVDKDVYLTIPRGRM
                     KDLKASYVLNSSELHAPLQKNQVVGTINFQLDGKTIEQRPLVVLQEIPEGNFFGKIID
                     YIKLMFHHWFG"
     gene            13911..14174
                     /gene="ybeD"
                     /locus_tag="ACHOSC_RS12575"
                     /old_locus_tag="pgaptmp_002515"
     CDS             13911..14174
                     /gene="ybeD"
                     /locus_tag="ACHOSC_RS12575"
                     /old_locus_tag="pgaptmp_002515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF493 family protein YbeD"
                     /protein_id="HGU4548440.1"
                     /translation="MKTKLNELLEFPTPFTYKVMGQALPELVDQVVEVVQRHAPGDYT
                     PTVKPSSKGNYHSVSITINATHIEQVETLYEELGKIDIVRMVL"
     gene            14275..14916
                     /gene="lipB"
                     /locus_tag="ACHOSC_RS12580"
                     /old_locus_tag="pgaptmp_002516"
     CDS             14275..14916
                     /gene="lipB"
                     /locus_tag="ACHOSC_RS12580"
                     /old_locus_tag="pgaptmp_002516"
                     /EC_number="2.3.1.181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415163.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033819 - lipoyl(octanoyl) transferase
                     activity [Evidence IEA];
                     GO_process: GO:0009249 - protein lipoylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoyl(octanoyl) transferase LipB"
                     /protein_id="HGU4548441.1"
                     /translation="MYQDKILVRQLGLQPYEPISQAMHEFTDTRDDSTLDEIWLVEHY
                     PVFTQGQAGKAEHILMPGDIPVIQSDRGGQVTYHGPGQQVMYVLLNLKRRKLGVRELV
                     TLLEQTVVNTLAELGIEAHPRADAPGVYVGEKKICSLGLRIRRGCSFHGLALNVNMDL
                     SPFLRINPCGYAGMEMAKISQWKPEATTNNIAPRLLENILALLNNPDFEYITA"
BASE COUNT         3711 a         3827 c         3932 g         3573 t
ORIGIN      
        1 ataatgattt tcacgtcaag ttgctgcgat aatttatcaa tagatgagat tatcgaacgt
       61 gctgaaaaag gcgattgcga ggctcaatat attgttgggt tttattataa tcgcgatagc
      121 gcaattgatt ctccggacga cgaaaaagcc ttttactggc tgaagctggc cgctgagcaa
      181 ggtcattgtg aagcacagta ttccttaggg caaaagtata ccgaggataa aagccgtcat
      241 aaagataatg agcaagccat cttctggctg aaaaaagctg ccctacaagg ccatactttc
      301 gcttccaacg cccttggctg gacactggat cgtggagaag cccccaatta taaagaagcg
      361 gttgtctggt atcagatagc cgcggagagc ggaatgtctt atgcgcaaaa taatcttggg
      421 tggatgtaca gaaatggcaa cggagtcgca aaagactatg cgctggcatt tttctggtac
      481 aaacaagctg cattacaagg ccatagtgac gcgcaaaaca atctggccga tctttatgaa
      541 gacggaaaag gcgttgctca aaacaagaca ctcgccgcat tctggtattt gaaaagcgca
      601 cagcagggta atcggcacgc ccagtttcaa attgcatggg attataacgc tggcgaaggg
      661 gtggaccagg actataagca agcgatgtac tggtatctga aggctgccgc tcaggaaagc
      721 gtcggcgctt acgtcaacat cggttatatg tataaacacg gacaaggcgt tgagaaagat
      781 tatcaggctg cctttgaatg gtttacgaaa gccgctgaat gcaatgacgc cactgcctgg
      841 tataacctgg ccattatgta tcactacgga gaaggaagac ctgtcgatct ccgacaggct
      901 ctcgacctgt atcgtaaagt tcagtcatcc ggaaccagag atgtcagtca ggaaattcgt
      961 gagactgaag atttactgta gagataaaaa caagggccgg ggaatttcat tcggcccata
     1021 ttctctttat tgctgtgcga aatcagacta ttacggctca aacgggcgtc tctggaactg
     1081 gtcatgacca catttcgggc atagcgtcag cacttccggt gtgtagatcg ggagatggaa
     1141 gtgacatttc tcgcagacca gatttcccag cccgaccact tctccgctgt gataaacccc
     1201 atgatgattg aggtcctgga aaacttcgcg ccattcaagc tgcgttttat cggtgatatc
     1261 tgccagctcc tgccataagc tttctttaat cacccgcata aagacgctgt cagattcttc
     1321 tttcaggctc tcttcatagc tcatggcgaa ctcttccagg tcacgtctta cagctcgcgt
     1381 cagctcatcg acctcggttc gcgttaactc ccctgttttt attacgcgct cgcgcgcctg
     1441 ttccaccagt gcgtcgatat cacgttcgcc attgcgcagg cgttcgctca gtgacgcgac
     1501 cagttcacgg taatattgag caaccttgtt catcgtttca tctcccgggt cgttaactga
     1561 ctataataat agacgttctt tgttccttgc tttgctaata cgcccacaaa ctctgtaaat
     1621 taatgtatga ataatccggg catcatttca acgtcgagtg cgcgaaaggc tgttttgacg
     1681 cggacgtttg ggctatgcta tgcggatctg aaaaaccacc tcaacgctac atttgtagcc
     1741 gtattgaaaa caggaccact ggctgccatg caagagcaat accgcccgga agagatagaa
     1801 tccaaagtac agcttcattg ggatgagaag cgcacatttg aagtaaccga agacgagagc
     1861 aaagagaagt attactgcct gtctatgctt ccctatcctt ctggtcgact acacatgggc
     1921 cacgtacgta actacaccat cggtgacgtg atcgcccgct accagcgtat gctgggcaaa
     1981 aacgtcctgc agccgatcgg ctgggacgcg tttggtctgc ctgcggaagg cgcggcggtg
     2041 aaaaacaaca ccgctccggc accgtggacg tacgacaaca tcgcgtatat gaaaaaccag
     2101 ctcaaaatgc tgggctttgg ttatgactgg agccgcgagc tggcaacctg tacgccggaa
     2161 tactaccgtt gggaacagaa attcttcacc gagctgtata aaaaaggcct ggtatataag
     2221 aagacttctg cggtcaactg gtgcccgaac gaccagaccg tactggcgaa cgaacaagtt
     2281 atcgacggct gctgctggcg ctgcgatacc aaagttgaac gtaaagagat cccgcagtgg
     2341 tttatcaaaa tcactgctta cgctgacgag ctgctcaacg atctggataa actggatcac
     2401 tggccagaca ccgttaaaac catgcagcgt aactggatcg gtcgttccga aggcgtggag
     2461 atcaccttca acgttaacga ctatgacaac acgctgaccg tttacactac ccgcccggac
     2521 acctttatgg gttgtaccta cctggcggta gctgcgggtc atccgctggc gcagaaagcg
     2581 gcggaaaata atcctgaact ggcggccttt attgacgaat gccgtaacac caaagttgcc
     2641 gaagctgaaa tggcgacgat ggagaaaaaa ggcgtcgata ctggctttaa agctgttcac
     2701 ccattaacgg gcgaagaaat tcccgtttgg gcagcaaact tcgtattgat ggagtacggc
     2761 acgggcgcag ttatggcggt tccgggtcac gaccagcgcg actacgagtt tgcctctaaa
     2821 tacggcctga acatcaaacc agttatcctg gctgctgacg gctctgagcc agatctttct
     2881 cagcaagccc tgactgaaaa aggcgtgctg ttcaactctg gcgaattcaa cggtcttgac
     2941 catgaagcgg ccttcaacgc catcgccgat aaactgactg cgatgggcgt tggcgagcgt
     3001 aaagtgaact accgcctgcg cgactggggt gtttctcgtc agcgttactg gggcgcgccg
     3061 attccgatgg tgacgctgga agacggtacc gtaatgccga ccccggacga ccagctgccg
     3121 gtgatcctgc cggaagatgt ggtgatggac ggcattacca gcccgattaa agcagatccg
     3181 gagtgggcaa aaactaccgt taacggtatg ccagcgctgc gtgaaaccga cactttcgac
     3241 acctttatgg agtcctcctg gtactatgcg cgctacactt gcccagagta caaagaaggt
     3301 atgctggatt ccgaagcggc taactactgg ctgccggtgg atatctacat tggtggtatc
     3361 gaacacgcca ttatgcacct gctctacttc cgcttcttcc acaaactgat gcgtgatgca
     3421 ggcatggtga actctgacga accagcgaaa cagttgctgt gtcagggtat ggtgctggca
     3481 gatgccttct actatgttgg cgaaaacggc gaacgtaact gggtttcccc ggttgatgct
     3541 atcgttgaac gtgacgagaa aggccgtatc gtgaaagcga aagatgcggc aggccatgaa
     3601 ctggtttata ccggcatgag caaaatgtcc aagtcgaaga acaacggtat cgacccgcag
     3661 gtgatggttg aacgttacgg cgcggacacc gttcgtctgt ttatgatgtt tgcttctccg
     3721 gctgatatga ctctcgaatg gcaggaatcc ggtgtggaag gggctaaccg cttcctgaaa
     3781 cgtgtctgga aactggttta cgagcacaca gcaaaaggtg atgttgcggc actgaacgtt
     3841 gatgcgctga ctgaagatca gaaagcgctg cgtcgcgatg tgcataaaac gatcgctaaa
     3901 gtgaccgatg atatcggccg tcgtcagacc ttcaacaccg caattgcggc gattatggag
     3961 ctgatgaaca aactggcgaa agcaccaacc gatggcgagc aggatcgcgc tctgatgcag
     4021 gaagcgctgc tggccgttgt ccgtatgctt aacccgttca ccccgcacat ctgcttcacg
     4081 ctgtggcagg aactgaaagg cgaaggcgat atcgacaacg cgccgtggcc ggttgctgac
     4141 gaaaaagcga tggtggaaga ctccacgctg gtcgtggtgc aggttaacgg taaagtccgt
     4201 gccaaaatca ccgttccggt ggacgcaacg gaagaacagg ttcgcgaacg tgctggccag
     4261 gaacatctgg tagcaaaata tcttgatggc gttaccgtgc gtaaagtgat ttacgtacca
     4321 ggtaaactcc tcaatctggt cgttggctaa gcgcgggagg aagcgtgcga tatctggcaa
     4381 cattgttgtt atctctggct gtgttaatca ccgccgggtg tggctggcat ctgcgtgata
     4441 ccacgcaggt tccttccact atgaaggtca tgatcctgga ctcaggcgat ccgaacgggc
     4501 cattaagccg tgcggtgcgt aaccagttac gtctgaatgg tgtcgagttg cttgataaag
     4561 aaaccacgcg taaggacgtt ccatccctgc gtttgggtaa agtgagcatc gcgaaagata
     4621 ccgcatcggt attccgtaac ggtcaaacag cagagtatca gatgatcatg acggtgaatg
     4681 cgaccgtgtt gatccccggc cgtgatatct acccgattag tgccaaagtc ttccgttcgt
     4741 tctttgataa cccgcaaatg gcgttagcga aagataacga acaagacatg atcgtaaaag
     4801 agatgtacga ccgtgctgcc gaacagctga ttcgtaagct gccaagcatc cgtgctgcgg
     4861 atattcgttc cgacgaagaa cagacgtcga ccacaacgga tactccggca acgcctgcac
     4921 gcgtctccac cacgctgggt aactgatgat tcggttgtac ccggaacaac tccgcgcgca
     4981 gctcaatgaa gggctgcgcg cggcgtatct tttacttggt aacgatcctc tgttattgca
     5041 ggaaagccag gacgctgttc gtcaggtagc tgcggcacaa ggattcgaag aacaccacac
     5101 tttttccatt gatcccaaca ctgactggaa tgcgatcttt tcgttatgcc aggctatgag
     5161 tctgtttgcc agtcgacaaa cgctattgct gttgttacca gaaaacggac cgaatgcggc
     5221 gatcaatgaa caacttctca cactcaccgg acttctgcat gacgacctgc tgttgatcgt
     5281 ccgcggtaat aaattaagca aagcgcaaga aaatgccgcc tggtttactg ctcttgcgaa
     5341 tcgcagcgtg caggtgacct gtcagacacc ggagcaggct cagcttcccc gctgggttgc
     5401 tgcgcgcgca aaacagctca acttagaact ggatgacgct gcaaatcagg tgctctgcta
     5461 ctgttatgaa ggtaacctgc tggcgctggc tcaggcactg gagcgtttat cgctgctctg
     5521 gccagacggc aaattgacat taccgcgcgt tgaacaggcg gtgaatgatg ccgcgcattt
     5581 cacccctttt cattgggttg atgctttgtt gatgggaaaa agtaagcgcg cgttgcatat
     5641 tcttcagcaa ctgcgtctgg aaggcagcga accggttatt ttgttgcgca cattacaacg
     5701 tgaactgttg ttactggtta acctgaaacg ccagtctgcc catacgccac tgcgtgcgtt
     5761 gtttgataag catcgggtat ggcagaaccg ccggggcatg atgggcgagg cgttaaatcg
     5821 cttaagtcag acgcagttac gtcaggccgt gcaactcctg acacgaacgg aactcaccct
     5881 caaacaagat tacggtcagt cagtgtgggc agagctggaa gggttatctc ttctgttgtg
     5941 ccataaaccc ctggcggacg tatttatcga cggttgatat gaaatcttta caggctctgt
     6001 ttggcggcac ctttgatccg gtgcactatg gtcatcttaa acccgtggaa acgctggcga
     6061 atttgattgg tctgacgcgg gtcacaatca tccctaataa tgttcctccg catcgtcccc
     6121 agccggaagc gaacagcgtg cagcgtaaac acatgcttga actggcgatt gccgacaagc
     6181 cattatttac tcttgatgaa cgcgagctaa agcgcaatgc cccctcttac actgcgcaaa
     6241 cactgaaaga gtggcggcag gaacaagggc cggacgtgcc gctggcgttt attattggtc
     6301 aggattcatt gctgaccttt ccgacctggt acgaatacga aacgatactc gacaatgcac
     6361 atttgatcgt ctgtcggcgt ccaggttacc cacttgaaat ggcgcaaccg caataccagc
     6421 aatggctgga agatcatttg acacataacc cggaagatct tcaccttcag cctgccggta
     6481 aaatttatct ggctgaaacg ccgtggttta acatctcggc gaccatcatc cgcgaacgtt
     6541 tgcaaaacgg tgaatcgtgt gaggatttat tgccggaacc ggtactgact tacattaacc
     6601 aacaaggctt gtatcgctga tacctgtcgt tacggagggc atgatgcgac tgtggttaat
     6661 tcgtcatggt gaaacgcaag cgaatatcga tggtctttac agcggtcatg cgcccacccc
     6721 cctgaccgcg cgcggtattg agcaagcgca aaatctgcat acactgctag atgatgtttc
     6781 ctttgacctg gttttatgca gtgaactgga acgggcacag cataccgcgc gacgggttct
     6841 cagtgaccgc cagctccccg tgcaaatcat ccctgaactc aacgaaatgt tttttggcga
     6901 ctgggagatg cgacatcatc gcgacctcat gcaagaagat gccgaaaact atagcgcgtg
     6961 gtgcaatgac tggcagcatg ctatccccac gaacggtgaa ggatttcagg cattttcgca
     7021 acgtgtggaa agatttatcg caaggcttag tgaatatcag cactatcaga atattttaat
     7081 cgtcagccat cagggcgtac tgagtctgtt aattgctcgt ctaattggca tgcctgccga
     7141 atctatgtgg cattttcgcg ttgaccaggg gtgttggagc gccattgata tcaaccaaaa
     7201 attcgcgacg ctacgcgtcc tcaatagccg tgccatcggg gtcgagaatg catgactttt
     7261 ctgttttttt acgggtaagc cgcaacgacc attgacagcc ccgggcaggc tgatattctc
     7321 cgcagccaga ctttttccgc cagacacgac tttgtagaaa ttgttttaca aaaatggcga
     7381 tgcaatctgc ggcgcggggt gggatgatag cccactttcg aaagccgatt cggcgacaat
     7441 tgtcccgaaa tcgcctctgg ttcaggtata ctgacagacc atttttatct atttgattca
     7501 cccaggggga aaacttgcag ggtaaagcac tccaggattt tgttatcgac aaaattgatg
     7561 acctcaaagg tcaggacatc atcgccttag acgttcaggg caaatccagc atcaccgact
     7621 gcatgatcat ctgtacgggt acgtccagcc gtcatgttat gtccattgct gaccacgttg
     7681 tgcaggagtc tcgcgcagcg ggcctgttac cgctcggcgt agaaggtgaa aacagcgccg
     7741 actggattgt cgtggatttg ggcgatgtga ttgtccatgt catgcaggaa gagagccgtc
     7801 gcctgtatga actggaaaaa ctctggagtt aatgcgtgaa gctgcaactt gtcgccgtgg
     7861 gaacgaaaat gccggactgg gtacaaaccg gttttaccga gtacctgcgt cgttttccga
     7921 aagatatgcc cttcgagctg attgaaattc cggccggaaa acgcggcaag aatgcggaca
     7981 tcaagcgcat actcgacaaa gagggtgagc agatgttggc ggccgcaggc aaaaaccgca
     8041 ttgtcaccct cgatattcca ggcaagccct gggatacgcc gcagttagcc gctgagctgg
     8101 aacgctggaa gctggatggt cgcgacgtca gtctactgat tggcgggcct gaagggttgt
     8161 cgcctgcctg taaagcggcg gcggagcaga gctggtcgct gtcggcgctt accctccccc
     8221 atccgctggt tcgcgtgctg gtcgcagaga gtctgtaccg ggcgtggagc atcaccacca
     8281 accatcctta tcaccgtgag tgataaagga gctttgagta gaaaacgcag cggatgaaac
     8341 tacagaactc ttttcgcgac tatacggcag agtccgcgct gtttgtgcgc cgggcgctgg
     8401 tcgccttttt ggggattttg ctgctgaccg gcgtgcttat cgccaacctg tataatctgc
     8461 aaattgttcg ctttaccgat taccagaccc gctctaatga aaaccgcatt aagctggtgc
     8521 ctatcgcgcc cagccgcggc attatctacg accgtaacgg tatccctctg gcactcaacc
     8581 gcactatcta ccagatagaa atgatgccgg agaaagtcga taacgtgcag caaacgctgg
     8641 acgctttgcg cagcgtggtt gatctgaccg atgacgatat tgctgcattc cgaaaagagc
     8701 gcgcacgttc acaccgtttc acctctattc ccgtgaaaac taacctgacc gaagtacaag
     8761 tagctcgctt tgccgtcaat cagtaccgtt ttccgggtgt cgaagttaaa ggctataaac
     8821 gtcgttacta tccttacggt tcggcgttga cccacgtcat cggctatgtg tcgaaaatca
     8881 acgataaaga cgtcgaacgc ctgaataatg acggcaaact ggccaactat gcggcaacgc
     8941 atgatatcgg taagctgggc attgagcgtt actatgaaga cgtgctgcac ggtcagaccg
     9001 gttatgaaga ggttgaagtt aacaaccgtg ggcgtgttat ccgccagtta aaagaagtac
     9061 caccgcaagc cggacacgat atttacctga cgctggatct caaactccag caatatattg
     9121 aaacgctgct ggcgggtagc cgcgcagctg tggtagtcac cgatccgcgt acaggtgggg
     9181 tgctggcact ggtttccacg cctagttatg acccaaacct gtttgttgac ggtatctcca
     9241 gcaaagatta ttccgccttg ctgaacgacc cgaatacacc gctggtgaac cgcgccacac
     9301 agggggttta tcctcctgcg tctacagtta aaccctatgt ggcggtttcg gcattaagcg
     9361 ccggggtgat cacgcgcaat acgacgctgt ttgacccagg ctggtggcaa ctaccagggt
     9421 cggaaaaacg ttatcgtgac tggaaaaaat ggggccacgg gcgtctgaat gtcacaagat
     9481 cgctggaaga atctgcggat accttcttct atcaggtggc ctacgatatg gggatcgatc
     9541 gcctctccga atggatgggt aaattcggtt atggtcatta caccggtatc gacctggcgg
     9601 aagaacgttc cggcaacatg cctacccgcg aatggaaaca gaaacgcttt aaaaaaccgt
     9661 ggtatcaggg tgacaccatt ccggttggta tcggccaggg ttactggaca gcgaccccaa
     9721 tccagatgag taaggcactg atgatcctga ttaatgacgg tatcgtgaag gttccgcatt
     9781 tgctgatgag caccgccgaa gacggcaaac aggtgccatg ggtacaaccg catgaaccgc
     9841 ccgtcggcga tattcattcc ggttactggg agctggcgaa agacggtatg tacggtgttg
     9901 ctaaccgccc taacggtacg gcgcataaat actttgccag cgcaccgtac aaaattgcgg
     9961 cgaaatctgg taccgctcag gtcttcggtc tgaaagcgaa cgaaacctat aatgcgcaca
    10021 aaattgccga gcgtttacgt gaccacaaac tgatgaccgc ctttgcgcca tacaacaatc
    10081 cgcaagtggc tgtcgccatg attctggaga acggcggcgc gggtccggcg gttggtacgc
    10141 tgatgcgcca gatcctcgac cacattatgc tgggtgataa caataccgat ctgcctgcgg
    10201 aaaatcctgc ggttgccgca gcggaggacc attaatcatg acggataatc cgaataaaaa
    10261 aacattctgg gataaagtcc atctcgatcc cacaatgctg ctgatcttac tggcattgct
    10321 ggtttacagc gccctggtta tctggagcgc cagcggtcag gatattggca tgatggagcg
    10381 taaaataggc caaatcgcga tgggtctggt catcatggtg gtgatggcgc aaattcctcc
    10441 acgcgtttat gaaggctggg ccccctatct ctatatcatc tgtattattt tgctggtggc
    10501 ggtagatgct ttcggtgcca tctctaaagg cgctcaacgc tggctggacc tcggtattgt
    10561 tcgttttcag ccgtcggaaa ttgccaaaat agccgtacca ctgatggttg cgcgctttat
    10621 caaccgcgac gtttgcccgc catcgttgaa gaacactggc atcgcgctgg tgctgatatt
    10681 tatgcccacg ctgctggtgg ctgcacagcc tgacctggga acatcaatcc tcgttgcgct
    10741 ttccggtctg tttgtactgt tcctctctgg ccttagctgg cgtctgattg gcgtcgcagt
    10801 agtgctggtg gcggcgttca ttcctattct gtggttcttc ctgatgcatg attaccagcg
    10861 ccagcgcgta atgatgctcc tggacccgga atcagaccca ctcggcgcgg gctatcacat
    10921 tattcagtct aaaattgcta ttggctccgg cggattacgc ggcaaaggct ggctgcatgg
    10981 cactcagtca caacttgaat ttctacccga acgccatacc gactttatct tcgcggtact
    11041 ggcggaagag ctgggattag tgggcattct gattctgctc gctctctaca ttctgctgat
    11101 catgcgcggg ctgtggatag ccgccagagc gcaaaccacc tttggtcgcg tcatggctgg
    11161 cggcttaatg ctgatattat tcgtttatgt cttcgtaaat attggtatgg taagcggtat
    11221 tctgccggtt gtaggggttc cgctcccact ggtcagttat ggaggatcag cgctaattgt
    11281 gctgatggct gggttcggga ttgtaatgtc aatccacacc cacaggaaaa tgttgtcgaa
    11341 aagcgtgtaa gaggtgcgca atgcgtaagc agtggctcgg gatctgcatc gcggcaggaa
    11401 tgctcgcggc atgtacaagc gatgatggtc agcaacagac ggtaagtgta ccgcagcctg
    11461 cggtatgtaa cggccccata gttgaaatta gcggggcgga cccgcgtttc gaaccactga
    11521 acgcgacggc aaatcaggat taccagcgcg acggtaaaag ctacaaaatc gtgcaggatc
    11581 cgtctcgatt tagccaggcg ggactggcgg caatctatga tgccgaacca ggcagtaacc
    11641 tgacggcctc tggcgaagct ttcgatccga cacagttgac ggcggcccat ccaacgcttc
    11701 cgatccccag ctacgccaga atcactaacc aggctaacgg gcgaatgatc gtggtgcgca
    11761 ttaatgatcg cggcccctac ggcaacgacc gcgtaatttc gctttctcgc gcagcagctg
    11821 accgtcttaa cacgtcaaac aacaccaaag ttcgtatcga tccgattatt gtcgcccagg
    11881 atggttcgct ttctggtcct ggtatggctt gtaccacagt cgccaaacag acttacgccc
    11941 tgcctgcacc tcccgattta agcggtggcg cgggggcaag ttcagtgtct ggcccgcagg
    12001 gtgacattct tccggtcagt aactcgacgc taaaaagcga agatccgacc ggcgcgccgg
    12061 taaccagtag cggtttcctc ggcgctccaa cgaccttagc gcctggtgta ctggaaagca
    12121 gcgaaccgac acctgcacca cagcccgttg ttacagctcc gtcgacaacg cctgcaacct
    12181 cgcctgcaat ggtgacaccg caagccgcct cgcaaagcgc cagcggcaac tttatggtgc
    12241 aagtcggggc cgttagcgat caggctcgtg cgcaacagta ccaacagcaa ctgggacaga
    12301 agttcggcgt ccccggtcgc gtaactcaaa atggcgcggt ctggcgtatc cagcttggcc
    12361 cattcgccag taaagccgaa gccagtacct tgcagcaacg tttgcaaacc gaagcccaat
    12421 tacagtcatt tattaccacc gcgcagtagc gtaaagcagg catctgatgt gtcaatttct
    12481 gtaaatgacg ttaacaaagt cacgcggaaa gtcagatgcc tgccggtagt gcatttgcta
    12541 tagtagggca ctttttttaa ttccatcacg gatgtcgtag ttcagaccat gaataccatt
    12601 ttttccgctc gtatcatgaa gcgcctggcg ctcaccacgg ctctttgcac agcctttatc
    12661 tctgctgcac atgccgatga cctgaatatc aaaactatga tcccgggtgt accgcagatc
    12721 gatgcggagt cctacatcct gattgactat aactccggca aagtgctcgc cgaacagaac
    12781 gcagatgtcc gccgcgatcc tgctagcctg accaaaatga tgaccagtta cgttatcggc
    12841 caggcaatga aagccggtaa atttaaagaa actgatttag tcactatcgg caacgacgca
    12901 tgggccaccg gtaacccggt gtttaaaggt tcttcgctga tgttcctcaa accgggcatg
    12961 caggttccgg tttctcagct gatccgcggt attaacctgc aatcgggtaa cgatgcttgc
    13021 gtcgccatgg ccgattttgc cgctggtagc caggacgctt ttgttggctt gatgaacagc
    13081 tacgttaacg ccctgggcct gaaaaacacc cacttccaga cggtacatgg tctggatgct
    13141 gatggtcagt acagctccgc gcgagatatg gcgctgatcg gccaggcatt gatccgtgac
    13201 gtaccgaatg aatactcgat ctataaagaa aaagaattta cgtttaacgg tattcgccag
    13261 ctgaaccgta acggcctgtt atgggataac agcctgaatg tcgacggcat caaaaccgga
    13321 cacactgaca aagcaggtta caaccttgtt gcttctgcga ctgaaggcca gatgcgcttg
    13381 atttctgcgg tgatgggcgg acgtactttt aaaggccgtg aagccgaaag taaaaaactg
    13441 ctaacctggg gcttccgttt cttcgaaacc gttaacccac tgaaagtagg taaagagttc
    13501 gcctctgaac cggtttggtt tggtgattct gatcgcgctt cgttaggggt tgataaagac
    13561 gtgtacctga ccattccacg tggtcgcatg aaagatctga aagccagcta tgtgctgaac
    13621 agcagtgaat tgcatgcgcc gctgcaaaag aatcaggtcg tcggaactat caacttccag
    13681 cttgatggca aaacgatcga gcaacgcccg ctggttgtgt tgcaagaaat cccggaaggt
    13741 aacttcttcg gcaaaatcat tgattacatt aaattaatgt tccatcactg gtttggttaa
    13801 aaattgaaca cttgaaagtg taatttccgt ccccatatac taagcatcag taaaaaaact
    13861 cccgccttct ggcgggagtt gctatttaat tacgttacgc cggagctgac atgaaaacca
    13921 aacttaacga actgcttgaa ttccctactc cttttactta caaagttatg gggcaggcgt
    13981 tacctgagct ggttgatcag gtggttgaag tggtacagcg ccatgcgcca ggtgactaca
    14041 ccccaacggt aaaaccaagc agcaaaggca actaccactc ggtatctatc actatcaacg
    14101 ccactcatat cgagcaggtt gaaacactgt atgaagaact gggcaaaatc gatattgtcc
    14161 gcatggttct gtaactcgct tctccgttac ccggctcctg gtcgggtaac tcccctctcc
    14221 tcgctgtgat atacttttcc cccactttta ctcattctcc acggagatgc cgttttgtat
    14281 caggataaaa ttcttgtccg ccagctcggt cttcagcctt acgagccaat ctcccaggct
    14341 atgcatgaat tcaccgatac ccgcgatgat agtacccttg atgaaatctg gctggtcgag
    14401 cactatccgg tattcaccca aggtcaggca ggaaaagcgg agcacatttt aatgccgggt
    14461 gatattccgg tgatccagag cgatcgcggt gggcaggtga cttatcacgg gccgggacaa
    14521 caggtgatgt atgtgttgct taacctgaaa cgccgtaaac tcggtgtgcg tgaactggtg
    14581 accttgcttg agcaaacagt ggtgaatacc ctggctgaac tgggtataga agcgcatcct
    14641 cgggctgacg cgcctggtgt ctatgtcggg gaaaagaaaa tttgctcact gggtttacga
    14701 attcgacgcg gttgttcatt ccacggtctg gcattaaacg tcaatatgga tctttcacca
    14761 tttttacgta ttaatccttg tgggtatgcc ggaatggaaa tggctaaaat atcacaatgg
    14821 aaacccgaag cgacgactaa taatattgct ccacgtttac tggaaaatat tttagcgcta
    14881 ctaaacaatc cggacttcga atatattacc gcttaattcc atacatcaat ggcccaattt
    14941 acactgggtc attactcata atacctgcta atgctttaca tctccttcgc gtttgtatat
    15001 tttctttatc acttgcagaa atgcggtgcc acgccttgct ccg
//
LOCUS       DAZKHF020000100        14940 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2201S31241
            SAMN43267800-rid25106413.denovo.101, whole genome shotgun sequence.
ACCESSION   DAZKHF020000100 DAZKHF020000000
VERSION     DAZKHF020000100.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267800
            Sequence Read Archive: SRR30307263
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 14940)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14940)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 14940)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350581.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350581.2
            Genome Coverage       :: 151x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:21:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,652
            CDSs (total)                      :: 5,557
            Genes (coding)                    :: 5,320
            CDSs (with protein)               :: 5,320
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 237
            CDSs (without protein)            :: 237
            Pseudo Genes (ambiguous residues) :: 0 of 237
            Pseudo Genes (frameshifted)       :: 102 of 237
            Pseudo Genes (incomplete)         :: 130 of 237
            Pseudo Genes (internal stop)      :: 52 of 237
            Pseudo Genes (multiple problems)  :: 41 of 237
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14940
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267800-rid25106413.denovo.101"
                     /serotype="O 26:H11"
                     /isolate="2201S31241"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-01"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            99..341
                     /gene="yjbE"
                     /locus_tag="ACHOSC_RS12585"
                     /old_locus_tag="pgaptmp_002517"
     CDS             99..341
                     /gene="yjbE"
                     /locus_tag="ACHOSC_RS12585"
                     /old_locus_tag="pgaptmp_002517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exopolysaccharide production protein YjbE"
                     /protein_id="HGU4548442.1"
                     /translation="MKKVLYGIFAISALAATSAWAAPVQVGEAAGSAATSVSAGSSSA
                     TSVSTVSSAVGVALAATGGGDGSNTGTTTTTTTSTQ"
     gene            455..1093
                     /locus_tag="ACHOSC_RS12590"
                     /old_locus_tag="pgaptmp_002518"
     CDS             455..1093
                     /locus_tag="ACHOSC_RS12590"
                     /old_locus_tag="pgaptmp_002518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418451.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjbF family lipoprotein"
                     /protein_id="HGU4548443.1"
                     /translation="MKRPALILICLLLQACSATTKELGNSLWDSLFGTPGVQLTDDDI
                     QNMPYASQYMQLNGGPQLFVVLAFAEDGQQKWVTQDQATLVTQHGRLVKTLLGGDNLI
                     EVNNLAADPLIKPAQIVDGATWTRTMGWTEYQQVRYATARSVFKWDGTDTVKVGSDET
                     LVRVLDEEVSTDQARWHNRYWIDSEGQIRQSEQYLGADYFPVKTTLIKAAKQ"
     gene            1090..1827
                     /locus_tag="ACHOSC_RS12595"
                     /old_locus_tag="pgaptmp_002519"
     CDS             1090..1827
                     /locus_tag="ACHOSC_RS12595"
                     /old_locus_tag="pgaptmp_002519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="capsule biosynthesis GfcC D2 domain-containing
                     protein"
                     /protein_id="HGU4548444.1"
                     /translation="MIKQTFVALLLSVGASSVFAAGTVKVFSNGSSEAKTLTGAEHLI
                     DLVGQPRLANSWWPGAVISEELATAAALRQQQALLTRLAEQGADSSTDDAAAINALRQ
                     QIQALKVTGRQKINLDPDIVRVAERGNPPLQGNYRLWVGPPPSTVTLFGLISHPGNQP
                     FTPGRDVASYLSGQNLLSGADRSYAWVVYPDGRTQKAPVAYWNKRHVEPMPGSIIYVG
                     LADSVWSETPDALNADILQTLTQRIPQ"
     gene            1827..3923
                     /locus_tag="ACHOSC_RS12600"
                     /old_locus_tag="pgaptmp_002520"
     CDS             1827..3923
                     /locus_tag="ACHOSC_RS12600"
                     /old_locus_tag="pgaptmp_002520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjbH domain-containing protein"
                     /protein_id="HGU4548445.1"
                     /translation="MKKRHLLSLLALGISMACYGETYPAPIGPSQSDFGGVGLLQTPT
                     ARMAREGELSLNYRDNDQYRYYSASVQLFPWLETTLRYTDVRTRQYSSVEAFSGDQTY
                     KDKAFDLKLRLWEESYWLPQVAVGARDIGGTGLFDAEYLVASKAWGPFDFTLGLGWGY
                     LGTSGNVKNPLCSASDKYCYRDNSYKQAGSIDGSQMFHGPASLFGGVEYQTPWQPLRL
                     KLEYEGNNYQQDFAGKLEQKSKFNVGAIYRVTDWADVNLSYERGNTFMFGVTLRTNFN
                     DLRPSYNDNARPQYQPQPQDAILQHSVVANQLTLLKYNAGLADPQIQAKGDTLYVTGE
                     QVKYRDSREGIIRANRIVMNDLPDGIKTIRITENRLNMPQVTTETDVASLKNHLAGEP
                     LGHETKVVQKRVEPVVPKSTEQGWYIDKSRFDFHIDPVLNQSVGGPENFYMYQLGVMG
                     TADLWLTDHLLTTGSLFANLANNYDKFNYTNPPQDSHLPRVRTHVREYVQNDVYVNNL
                     QANYFQHLGNGFYGQVYGGYLETMFGGAGAEVLYRPLDSNWAFGLDANYVKQRDWRSA
                     KDMMKFTDYSVKTGHLTAYWTPSFAQDVLVKASVGQYLAGDKGGTLEIAKRFDSGVVV
                     GGYATITNVSKEEYGEGDFTKGVYVSVPLDLFSSGPTRSRAAIGWTPLTRDGGQQLGR
                     KFQLYDMTSDRSVNFR"
     gene            complement(3970..4248)
                     /locus_tag="ACHOSC_RS12605"
                     /old_locus_tag="pgaptmp_002521"
     CDS             complement(3970..4248)
                     /locus_tag="ACHOSC_RS12605"
                     /old_locus_tag="pgaptmp_002521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4548446.1"
                     /translation="MKRNLIKVVKMKPYFAALMLSVSVLPAYAGPLGTADKADLPQSN
                     VSSPMMAQSLRQPDLQPISTDRKTECFRLYTPDRKPGVNCVPDGSTGH"
     gene            4249..4392
                     /locus_tag="ACHOSC_RS12610"
                     /old_locus_tag="pgaptmp_002522"
     CDS             4249..4392
                     /locus_tag="ACHOSC_RS12610"
                     /old_locus_tag="pgaptmp_002522"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4548447.1"
                     /translation="MFQRNIVDFSRIAGGWESPEWVIFELPALVRFVLPDAPPGAAYP
                     AYG"
     gene            4518..4928
                     /gene="psiE"
                     /locus_tag="ACHOSC_RS12615"
                     /old_locus_tag="pgaptmp_002523"
     CDS             4518..4928
                     /gene="psiE"
                     /locus_tag="ACHOSC_RS12615"
                     /old_locus_tag="pgaptmp_002523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate-starvation-inducible protein PsiE"
                     /protein_id="HGU4548448.1"
                     /translation="MTSLSRPRVEFISTILQTVLNLGLLCLGLILVVFLGKETVHLAD
                     VLFAPEQTSKYELVEGLVVYFLYFEFIALIVKYFQSGFHFPLRYFVYIGITAIVRLII
                     VDHKSPLDVLIYSAAILLLVITLWLCNSKRLKRE"
     gene            complement(4972..6447)
                     /gene="xylE"
                     /locus_tag="ACHOSC_RS12620"
                     /old_locus_tag="pgaptmp_002524"
     CDS             complement(4972..6447)
                     /gene="xylE"
                     /locus_tag="ACHOSC_RS12620"
                     /old_locus_tag="pgaptmp_002524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-xylose transporter XylE"
                     /protein_id="HGU4548449.1"
                     /translation="MNTQYNSSYIFSITLVATLGGLLFGYDTAVISGTVESLHTVFVA
                     PQNLSESAANSLLGFCVASALIGCIIGGALGGYCSNRFGRRDSLKIAAVLFFISGVGS
                     AWPELGFTSINPDNTVPVYLAGYVPEFVIYRIIGGIGVGLASMLSPMYIAELAPAHIR
                     GKLVSFNQFAIIFGQLLVYCVNYFIARSGDATWLNTDGWRYMFASECIPALLFLMLLY
                     TVPESPRWLMSRGKQEQAEGILRKIMGNALAAQAVQEIKHSLDHGRKTGGRLLMFGVG
                     VIVIGVMLSIFQQFVGINVVLYYAPEVFKTLGASTDIALLQTIIVGVINLTFTVLAIM
                     TVDKFGRKPLQIIGALGMAIGMFSLGTAFYTQAPGIVALLSMLFYVAAFAMSWGPVCW
                     VLLSEIFPNAIRGKALAIAVAAQWLANYFVSWTFPMMDKNSWLVTHFHNGFSYWIYGC
                     MGVLAALFMWKFVPETKGKTLEELEALWEPETKKTQQTATL"
     gene            complement(6819..7709)
                     /gene="malG"
                     /locus_tag="ACHOSC_RS12625"
                     /old_locus_tag="pgaptmp_002525"
     CDS             complement(6819..7709)
                     /gene="malG"
                     /locus_tag="ACHOSC_RS12625"
                     /old_locus_tag="pgaptmp_002525"
                     /EC_number="7.5.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001252048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="maltose ABC transporter permease MalG"
                     /protein_id="HGU4548450.1"
                     /translation="MAMVQPKSQKARLFITHLLLLLFIAAIMFPLLMVVAISLRQGNF
                     ATGSLIPEQISWDHWKLALGFSVEQADGRITPPPFPVLLWLWNSVKVAGISAIGIVAL
                     STTCAYAFARMRFPGKATLLKGMLIFQMFPAVLSLVALYALFDRLGEYIPFIGLNTHG
                     GVIFAYLGGIALHVWTIKGYFETIDSSLEEAAALDGATPWQAFRLVLLPLSVPILAVV
                     FILSFIAAITEVPVASLLLRDVNSYTLAVGMQQYLNPQNYLWGDFAAAAVMSALPITI
                     VFLLAQRWLVNGLTAGGVKG"
     gene            complement(7724..9268)
                     /gene="malF"
                     /locus_tag="ACHOSC_RS12630"
                     /old_locus_tag="pgaptmp_002526"
     CDS             complement(7724..9268)
                     /gene="malF"
                     /locus_tag="ACHOSC_RS12630"
                     /old_locus_tag="pgaptmp_002526"
                     /EC_number="7.5.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="maltose ABC transporter permease MalF"
                     /protein_id="HGU4548451.1"
                     /translation="MDVIKKKHWWQSDALKWSVLGLLGLLVGYLVVLMYAQGEYLFAI
                     TTLILSSAGLYIFANRKAYAWRYVYPGMAGMGLFVLFPLVCTIAIAFTNYSSTNQLTF
                     ERAQEVLLDRSWQAGKTYNFGLYPAGDEWQLALSDGETGKNYLSDAFKFGGEQKLQLK
                     ETTTQPEGERANLRVITQNRQALSDITAILPDGNKVMMSSLRQFSGTQPLYTLDGDGT
                     LTNNQSGVKYRPNNQIGFYQSITADGNWGDEKLSPGYTVTTGWKNFTRVFTDEGIQKP
                     FLAIFVWTVVFSLITVFLTVAVGMVLACLVQWEALRGKAVYRVLLILPYAVPSFISIL
                     IFKGLFNQSFGEINMMLSALFGVKPAWFSDPTTARTMLIIVNTWLGYPYMMILCMGLL
                     KAIPDDLYEASAMDGAGPFQNFFKITLPLLIKPLTPLMIASFAFNFNNFVLIQLLTNG
                     GPDRLGTTTPAGYTDLLVNYTYRIAFEGGGGQDFGLAAAIATLIFLLVGALAIVNLKA
                     TRMKFD"
     gene            complement(9422..10612)
                     /gene="malE"
                     /locus_tag="ACHOSC_RS12635"
                     /old_locus_tag="pgaptmp_002527"
     CDS             complement(9422..10612)
                     /gene="malE"
                     /locus_tag="ACHOSC_RS12635"
                     /old_locus_tag="pgaptmp_002527"
                     /EC_number="7.5.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000695396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015144 - carbohydrate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="maltose/maltodextrin ABC transporter
                     substrate-binding protein MalE"
                     /protein_id="HGU4548452.1"
                     /translation="MKIKTGARILALSALTTMMFSASALAKIEEGKLVIWINGDKGYN
                     GLAEVGKKFEKDTGIKVTVEHPDKLEEKFPQVAATGDGPDIIFWAHDRFGGYAQSGLL
                     AEITPDKAFQDKLYPFTWDAVRYNGKLIAYPIAVEALSLIYNKDLLPNPPKTWEEIPA
                     LDKELKAKGKSALMFNLQEPYFTWPLIAADGGYAFKYENGKYDIKDVGVDNAGAKAGL
                     TFLVDLIKNKHMNADTDYSIAEAAFNKGETAMTINGPWAWSNIDTSKVNYGVTVLPTF
                     KGQPSKPFVGVLSAGINAASPNKELAKEFLENYLLTDEGLEAVNKDKPLGAVALKSYE
                     EELAKDPRIAATMENAQKGEIMPNIPQMSAFWYAVRTAVINAASGRQTVDEALKDAQT
                     RITK"
     gene            10977..12092
                     /gene="malK"
                     /locus_tag="ACHOSC_RS12640"
                     /old_locus_tag="pgaptmp_002528"
     CDS             10977..12092
                     /gene="malK"
                     /locus_tag="ACHOSC_RS12640"
                     /old_locus_tag="pgaptmp_002528"
                     /EC_number="7.5.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011915502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="maltose/maltodextrin ABC transporter ATP-binding
                     protein MalK"
                     /protein_id="HGU4548453.1"
                     /translation="MASVQLQNVTKAWGEVVVSKDINLDIHEGEFVVFVGPSGCGKST
                     LLRMIAGLETITSGDLFIGEKRMNDTPPAERGVGMVFQSYALYPHLSVAENMSFGLKL
                     AGAKKEVINQRVNQVAEVLQLAHLLDRKPKALSGGQRQRVAIGRTLVAEPSVFLLDEP
                     LSNLDAALRVQMRIEISRLHKRLGRTMIYVTHDQVEAMTLADKIVVLDAGRVAQVGKP
                     LELYHYPADRFVAGFIGSPKMNFLPVKVTATAIDQVQVELPMPNRQQVWLPVESRDVQ
                     VGANMSLGIRPEHLLPSDIADVILEGEVQVVEQLGNETQIHIQIPSIRQNLVYRQNDV
                     VLVEEGATFAIGLPPERCHLFREDGTACRRLHKEPGV"
     gene            12164..13504
                     /gene="lamB"
                     /locus_tag="ACHOSC_RS12645"
                     /old_locus_tag="pgaptmp_002529"
     CDS             12164..13504
                     /gene="lamB"
                     /locus_tag="ACHOSC_RS12645"
                     /old_locus_tag="pgaptmp_002529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maltoporin LamB"
                     /protein_id="HGU4548454.1"
                     /translation="MMITLRKLPLAVAVAAGVMSAQAMAVDFHGYARSGIGWTGSGGE
                     QQCFQTTGAQSKYRLGNECETYAELKLGQEVWKEGDKSFYFDTNVAYSVAQQNDWEAT
                     DPAFREANVQGKNLIEWLPGSTIWAGKRFYQRHDVHMIDFYYWDISGPGAGLENIDVG
                     FGKLSLAATRSSEAGGSSSFASNNIYDYTNETANDVFDVRLAQMEINPGGTLELGVDY
                     GRANLRDNYRLVDGASKDGWLFTAEHTQSVLKGFNKFVVQYATDSMTSQGKGLSQGSG
                     VAFDNEKFAYNINNNGHMLRILDHGAISMGDNWDMMYVGMYQDINWDNDNGTKWWTVG
                     IRPMYKWTPIMSTVMEIGYDNVESQRTGDKNNQYKITLAQQWQAGDSIWSRPAIRVFA
                     TYAKWDEKWGYDYNGDSKVNPNYGKAVPADFNGGSFGRGDSDEWTFGAQMEIWW"
     gene            13747..14667
                     /gene="malM"
                     /locus_tag="ACHOSC_RS12650"
                     /old_locus_tag="pgaptmp_002530"
     CDS             13747..14667
                     /gene="malM"
                     /locus_tag="ACHOSC_RS12650"
                     /old_locus_tag="pgaptmp_002530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="maltose operon protein MalM"
                     /protein_id="HGU4548455.1"
                     /translation="MKMNKSLIALCLSAGLLASAPGISLADVNYVPQNTSDAPAIPSA
                     ALQQLTWTPVDQSKTQTTQLATGGQQLNVPGISGPVAAYSVPANIGELTLTLTSEVNK
                     QTSVFAPNVLILDQNMTPSAFFPSSYFTYQEPGVMSADRLEGVMRLTPALGQQKLYVL
                     VFTTEKDLQQTTQLLDPAKAYAKGVGNSIPDIPDPVARHTTDGLLKLKVKTNSSSSVL
                     VGPLFGSSAPAPVTVGNTAAPAVAAPAPAPVKKSEPMLNDTESYFNTAIKNAVAKGDV
                     DKALKLLDEAERLGSTSARSTFISSVKGKG"
BASE COUNT         3694 a         3850 c         3937 g         3459 t
ORIGIN      
        1 tccccgcgcc gtaattcgca tgctttagtt gtgtatactc gatcccgccc gaaatgtttt
       61 tgggtaaatc tccattcatt caatgaaggg aaattgttat gaaaaaagtt ctgtatggca
      121 tttttgccat atctgcgctt gcggcgactt ctgcgtgggc tgcacctgta caggtgggcg
      181 aagcggcagg gtcggcagca acgtcggttt cggcggggag ttcctccgct accagcgtca
      241 gcaccgtaag ctcggcggtg ggtgtcgcgc tcgcggcaac cggtggcggt gatggttcta
      301 ataccgggac caccacaacc acgaccacca gtacccagta ataaagtatg tatccccaaa
      361 ataatttgag tcattgcatc tgtggcttga agtatgaagg gatttaccat aaccacactc
      421 cggtgtggtt attctgcccc tctggagaag agtcgtgaag cgacctgcac tcattcttat
      481 ctgcctgcta ttacaggcct gttcagccac gactaaagag ctgggcaatt cactgtggga
      541 cagtctgttc ggcacgccag gcgtacagct gacggacgat gatattcaaa atatgcccta
      601 cgccagccag tacatgcagc ttaatggcgg gccgcaattg tttgtggtgc tggccttcgc
      661 tgaagacgga caacaaaaat gggtcactca ggatcaagcc actcttgtca cacaacatgg
      721 tcgtctggtg aagactttgc ttggcggcga caacctgatt gaagtgaata accttgccgc
      781 cgacccgctg attaaacccg cacaaattgt tgatggtgca acatggacgc gcacgatggg
      841 ctggaccgag taccagcagg tacgctacgc caccgcacgc tcagtcttca aatgggatgg
      901 caccgatacc gtcaaagtcg gcagcgatga aaccctggtt cgcgtgctgg acgaagaagt
      961 ctccaccgac caggcgcgct ggcataaccg ctactggatc gacagcgaag ggcaaattcg
     1021 ccagtcggaa cagtatctcg gcgcggatta tttcccggtg aaaaccactc tcatcaaggc
     1081 ggcaaaacaa tgattaaaca aacttttgtc gcgttgcttt tgagtgtggg agcgtcatcg
     1141 gtcttcgcgg caggaaccgt caaggtgttc agcaatggca gcagtgaggc caaaacacta
     1201 acaggcgcag agcatttaat cgatctggta ggccaaccgc ggctggcaaa cagctggtgg
     1261 cccggtgcgg tgattagcga agagctggca acggcggcag cattgcgtca gcagcaggcg
     1321 ttgctgacac ggctggcaga acagggcgca gattccagca ccgacgatgc cgccgccatt
     1381 aacgccttac gccagcaaat ccaggcattg aaagtgacag gcaggcaaaa aatcaatctt
     1441 gatcccgata tcgtccgcgt tgccgaacgc ggtaacccgc cgttgcaggg caactacagg
     1501 ctgtgggtcg gaccaccgcc gtccacggtc acgttgttcg ggcttatcag ccatcctggc
     1561 aatcagccat tcactccagg tcgcgacgtg gcgagctatc tctccgggca gaacctgctt
     1621 agcggtgcgg atcgcagcta cgcgtgggtc gtttacccgg acggacgcac gcaaaaagcg
     1681 ccggtggctt actggaacaa gcgtcacgta gagccgatgc ccggcagcat tatttatgtt
     1741 ggcctcgcgg actccgtctg gagtgagacg cctgatgccc ttaacgccga cattcttcag
     1801 actctgacgc agcggatacc ccaataatga aaaaaagaca tctgcttagc ttactggcgc
     1861 tgggcattag catggcttgc tacggcgaaa catatcctgc gcccattggc ccgtcgcagt
     1921 cggatttcgg tggcgtagga ttattacaaa ctcccaccgc gcgtatggca cgggaagggg
     1981 agttgagtct gaactatcgc gataacgatc agtaccgtta ttactcagct tcagtgcaac
     2041 tcttcccgtg gctggaaaca acgctgcgct acaccgacgt gcgcacccgg cagtacagca
     2101 gcgtcgaagc gttctctggc gatcaaacgt ataaagataa agccttcgat ctcaaactgc
     2161 gtttgtggga agagagttat tggctgccgc aagtggcggt tggtgcgcgg gatatcggcg
     2221 gtacggggct gtttgatgcg gaatatcttg ttgccagcaa agcctggggg ccgttcgatt
     2281 ttacgctcgg cctgggctgg ggatatctgg gcaccagcgg taatgtgaaa aatccgctct
     2341 gttcagccag tgataaatat tgctatcgcg ataacagcta caaacaggcg ggatctatcg
     2401 acggtagcca gatgttccac ggtcctgcct cactgtttgg cggcgtggaa taccagacgc
     2461 cctggcaacc gctgcgcctg aaactggagt atgaaggcaa taattatcag caggattttg
     2521 ccgggaagct ggagcaaaaa agtaagttta acgtcggtgc gatttatcgc gttaccgatt
     2581 gggccgacgt taaccttagc tatgaacgtg gcaacacctt tatgtttggc gttacgttgc
     2641 gcaccaactt taacgatctg cgcccgtctt acaacgataa cgcccgcccg caatatcaac
     2701 cgcagccgca ggatgccatt ttgcagcatt cggtggtggc gaatcagtta acgttgctga
     2761 aatacaatgc cggacttgcc gatccgcaga tccaggcgaa aggcgatacg ctgtatgtta
     2821 ccggcgagca ggtgaaatat cgtgattcgc gcgaagggat catccgtgcg aatcggatcg
     2881 tgatgaacga tctgccggat gggatcaaaa cgatccgcat tacagaaaat cgccttaaca
     2941 tgccgcaggt gacgacggaa accgatgtcg ccagcttgaa aaatcatctc gccggggagc
     3001 cgctgggcca tgaaacgaag gtggtgcaaa aacgcgtcga gccagtggtt ccgaagtcca
     3061 ccgagcaggg atggtatatc gacaaatcac gctttgattt ccatatcgat ccggtgctga
     3121 accagtcggt cggtggcccg gaaaactttt acatgtatca gctgggcgtg atgggaacgg
     3181 cagatttgtg gctgacggac catctgctga ccaccggcag cctgtttgca aatcttgcca
     3241 acaactacga caagtttaac tacaccaatc cgccgcagga ctcgcactta ccgcgcgttc
     3301 gtacccatgt gcgcgagtat gtgcagaacg atgtctatgt gaataacctg caagccaact
     3361 acttccagca tctgggcaac ggcttctacg gtcaggtcta tggaggttat ctcgaaacca
     3421 tgtttggcgg tgccggggca gaagtgttgt atcgtccgct ggacagcaac tgggcgtttg
     3481 gtctggatgc caactacgtt aaacagcgcg actggcgtag tgcaaaagat atgatgaaat
     3541 tcaccgacta cagcgtgaaa accggacatc tgaccgccta ctggacgcca tctttcgccc
     3601 aggacgtgtt ggtgaaagcg agcgtcgggc agtatctggc aggggataaa ggcggcacgc
     3661 tggagatcgc caaacgcttt gatagcggcg tggtggttgg cggctatgcc acgatcacga
     3721 atgtttcgaa agaggagtac ggcgaagggg acttcaccaa aggcgtgtat gtctctgtgc
     3781 cgttggatct cttctcgtct ggcccgacac gcagccgtgc ggcgattggc tggacgccgc
     3841 tgacgcgtga cggtggtcag caacttgggc gtaagttcca gttgtatgac atgaccagcg
     3901 accgtagcgt caatttccgc taagtcatgg gaaaggtgcc agttttcgca atcactggca
     3961 ccctcattct taatggcctg ttgagccatc aggaacgcag ttcacccccg gtttgcgatc
     4021 tggtgtgtaa aggcgaaaac attctgtctt cctgtccgtc gatatcggtt gcagatctgg
     4081 ttgcctgagc gactgggcca tcattgggct ggagacattg ctttgcggta ggtcggcttt
     4141 atcagcagta ccgagcggac cagcataagc aggaagaaca gagactgata acatcaaagc
     4201 agcaaaataa ggcttcattt ttaccacctt tatcaggtta cgtttcattt gttccagagg
     4261 aacattgtcg atttttcgcg cattgctggt ggctgggaat cacctgaatg ggtgattttt
     4321 gaattaccgg ctttggtgcg gtttgtcttg ccggatgcgc cgccaggcgc ggcttatccg
     4381 gcctacgggt aggtatatcc ggctttggtg gaggcgcgct ccaaatccag gttgaacaaa
     4441 acatacacaa aaaatataga tctccgtcac atttttgcgt tatacaggaa gctcgccact
     4501 gtgaaggagg tactgctatg acgtcactct ctcgtccgcg cgtggagttt atctccacca
     4561 ttttgcagac cgtactcaat cttggcctgt tgtgcctcgg cctgattttg gttgtcttcc
     4621 tcggcaaaga aacggtgcat ctggctgatg tgctgttcgc gccagaacaa accagcaaat
     4681 atgagctggt agaagggctg gtggtttact ttctctattt cgaatttatc gcgctgattg
     4741 tgaagtactt tcagtccggt tttcacttcc cgctacgtta ctttgtctat attgggatca
     4801 ccgccattgt gcgcttgatc atcgtcgatc ataaatcgcc actggatgta ttgatctact
     4861 cggcagcgat cctgttactg gtgatcaccc tgtggctgtg taattcgaaa cggctgaagc
     4921 gggagtaaaa agacagcacg ccgaactggc gcggcgtgct ggacaggaag attacagtgt
     4981 agcagtttgt tgtgttttct tcgtttccgg ttcccagagg gcttccagct cctcaagggt
     5041 tttacctttg gtttccggga caaatttcca cataaatagt gctgccagaa cgcccataca
     5101 accgtaaatc cagtaggaga aaccgttgtg gaaatgggtc accagccagg agtttttgtc
     5161 catcatcggg aaggtccagg agacgaagta gttcgccagc cactgggccg ccaccgcgat
     5221 tgccagcgct ttaccacgaa tagcattcgg gaagatttcc gacagcagta cccagcatac
     5281 cggaccccag gacatggcaa aggcagcaac atagaacagc atcgacagta gcgccacaat
     5341 acccggtgcc tgagtgtaaa acgcggtacc gaggctaaac ataccgattg ccattccgag
     5401 cgcgccgata atttgcagtg gcttacgacc aaatttatcc accgtcataa ttgccagaac
     5461 ggtgaaggtg aggttgataa ctccgacaat aatggtctgc aacagcgcga tatccgtgct
     5521 ggcccccagc gttttgaaca cttccggcgc gtagtacaac accacattga tgccgacaaa
     5581 ttgctggaag atggagagca taacgccgat aacaatcacg cccacgccga acatcagcag
     5641 acgaccaccg gttttgcgtc catgatccag ggagtgttta atttcctgca ccgcctgggc
     5701 tgccaaagcg ttgcccataa ttttgcgcag gatgccttcc gcttgttctt gcttaccgcg
     5761 tgacatcagc cagcgagggc tttccggcac ggtataaagc agcattaaga acagcagcgc
     5821 agggatacat tccgaagcaa acatataacg ccagccgtca gtattcagcc aggtggcatc
     5881 gccggaacgg gcaataaaat agtttacgca gtaaactaaa agttggccga aaataatcgc
     5941 aaactggtta aaagagacca gcttcccacg aatatgtgca ggagccagtt ccgcaatata
     6001 cattggtgag agcattgagg ctaaaccaac accaataccg ccaataatgc gataaataac
     6061 aaattccggg acataacctg ccagataaac aggcactgtg ttgtccgggt ttatagaggt
     6121 aaaaccaagt tctggccagg cagaacctac accagaaata aaaaacagga cagcagcaat
     6181 cttaagtgaa tcacgacgac cgaagcggtt actgcaataa ccaccgaggg caccgccgat
     6241 gatgcaacca atcagagcgc tggctacgca aaaccctaac agggagttgg cagcggattc
     6301 acttaagttt tgtggagcaa caaagacggt atggagtgac tcaacagtac cggaaataac
     6361 ggcggtgtcg tagccaaata ataaaccacc taatgtagcg actaaggtaa tcgaaaatat
     6421 ataactggaa ttatactggg tattcattca gacctgcctt agaccattct gatcttttat
     6481 cagtgcattg atgttggaat tgtcaggcag ttaaggcaat tatcattttt tacaatgcgt
     6541 ttacgtaatg tcttttctgt gatcttaatt gtgataatta tctaaaattg atgaaaacaa
     6601 tactattgcc gtgactcaga gcacgaaaga gaattatcgt aagtgggaaa acaaataacg
     6661 taaaaataat aagctctatg atgggaatat taaccggcga acgattcaga ttgcagacga
     6721 aagaaaaaaa ggcgctccgt ggagcgccga ataacagtca caagttggga taacgtaagt
     6781 tgagggtgca gcggcataac attggcagaa caacatcttt aacctttcac accacctgcc
     6841 gtcaggccgt tgaccagcca gcgttgagcc agcaagaaga cgatggtgat cggtaatgca
     6901 gacatcacgg cagcggcggc aaagtcaccc cacaggtagt tttgcgggtt gaggtattgc
     6961 tgcatcccca cggccagggt gtagctgttt acgtcacgca gtaacagcga cgcgaccgga
     7021 acttcagtaa tggcagcgat aaacgacaag atgaatacca ccgccagaat cggtactgac
     7081 agcggcaaca ggacaaggcg gaaggcctgc cacggtgtcg caccatccag cgcagccgct
     7141 tcttccagcg aactatcgat ggtttcgaaa tagcctttga tggtccagac atgcagcgca
     7201 atcccaccca gatacgcgaa aattacgccg ccgtgagtat tcaggccaat gaatggaatg
     7261 tactcaccca gacgatcaaa caacgcatac aacgcgacca gtgaaagtac tgccgggaac
     7321 atctggaaaa tcagcattcc tttcagcagc gtcgctttgc ctggaaagcg catacgggcg
     7381 aaagcgtagg cgcaggtggt agagagcgcc acaatgccaa tcgcggaaat cccggcgacc
     7441 tttaccgagt tccacagcca cagcagtacc gggaatggcg gtggcgtaat gcgaccatca
     7501 gcctgttcaa cgctaaaacc taacgccagt ttccagtgat cccaggagat ttgctccggg
     7561 atcaggctac cggtcgcgaa gtttccctga cgcagcgaga tagcgacgac catcagcagc
     7621 gggaacataa tcgccgcgat aaaaagtagc agtagcaggt gagtaataaa caaacgtgct
     7681 ttttgcgatt tcggttggac cattgccatt tttgttatct cccttaatca aacttcattc
     7741 gcgtggcttt caggttcact atcgccagcg cacccaccag caggaagatc agcgtggcaa
     7801 ttgctgctgc cagaccgaag tcctgacccc cgccgccttc aaaagcgatg cggtaggtgt
     7861 agttaacaag caggtcggta taaccggctg gcgtggtcgt gccaagacga tccgggccgc
     7921 cgttggttaa cagttgaatc agcacgaagt tgttaaagtt aaaggcgaag ctggcgatca
     7981 tcagcggcgt cagcggttta atcagcagcg gcagcgtaat cttaaagaag ttctggaacg
     8041 gacctgcgcc atccattgct gaggcttcat acaaatcgtc cggaatcgct ttcagcaagc
     8101 ccatgcagag gatcatcatg tacggataac ccagccaggt attgacgata atcagcatcg
     8161 tgcgggcggt ggtcggatcg ctgaaccagg cgggcttcac gccaaacagc gcgctcaaca
     8221 tcatgttgat ttcaccgaag ctctggttaa acaacccttt gaaaatcaag attgaaatga
     8281 acgatggcac cgcgtagggc agaatcagca ggacgcgata gaccgctttg ccgcgcaacg
     8341 cttcccactg caccagacac gccagaacca tgccgaccgc caccgttaaa aagacagtga
     8401 tcagcgagaa caccacggtc cagacgaaaa tggcgaggaa cggtttctga atgccttcgt
     8461 cggtgaagac gcgggtaaag tttttccagc cagtagtcac ggtgtaaccg gggcttagct
     8521 tttcatcgcc ccagttgccg tcggcggtaa tggactggta aaagccaatt tggttattcg
     8581 gacgatattt cacgccgctc tgattattcg tcaacgtgcc gtcaccgtcg agtgtgtaga
     8641 gcggctgcgt gccagaaaac tggcgcaggg agctcatcat cactttgttg ccatccggca
     8701 gaatggcggt aatgtcactc agcgcctgac gattctgggt aatcacgcgc agattcgcgc
     8761 gttcgccttc gggctgggtg gtcgtttctt tcagttgcag tttttgctcg ccgccaaatt
     8821 taaaagcgtc ggagaggtaa tttttgccgg tttcgccgtc gctgagggct agttgccact
     8881 catcgcccgc cgggtaaaga ccaaaattat aggttttgcc tgcttgccag gagcgatcta
     8941 gcaacacttc ctgcgcacgt tcaaaagtca gctggttagt gctgctgtag ttggtgaagg
     9001 caatggcgat ggtgcagacc agagggaaga ggacgaataa tcccattcca gccattcccg
     9061 ggtaaacata gcgccaggcg taggctttac gattggcgaa aatatacagc cccgctgaac
     9121 tcaatatcag cgtggtaatg gcgaacaggt attccccttg tgcgtacatt aaaacaacaa
     9181 ggtaacccac cagcaggccg agcagaccta acactgacca tttcagcgcg tcgctttgcc
     9241 accaatgttt ctttttaatg acatccatgg ggttcttcct cattccagga cggataaggc
     9301 tttcacgcct tatccgacaa caactgcctg atgcgacgct gacgcgtctt atcaggccta
     9361 catacgtttc ggttttgtag gccggacaag gcgttcacgc cgcatccggc atttcacagc
     9421 attacttggt gatacgagtc tgcgcgtctt tcagggcttc atcgacagtc tgacgaccgc
     9481 tggcggcgtt gatcaccgca gtacgcacgg cataccagaa agcggacatc tgcgggatgt
     9541 tcggcatgat ttcacctttc tgggcgtttt ccatggtggc tgcaatacgt ggatctttcg
     9601 ccaactcttc ctcgtaagac ttcagcgcta cggcacccag cggtttgtct ttattaaccg
     9661 cttccagacc ttcgtcagtc agcagatagt tttcgaggaa ctctttcgcc agctctttgt
     9721 tcggactggc ggcgttaata ccggcgctca gcacgccaac gaacggttta gatggttgac
     9781 ccttgaaggt cggcagtacc gttacaccat aattcacttt gctggtgtcg atgttggacc
     9841 atgcccacgg gccgttgatg gtcatcgctg tttcgccttt attaaaggca gcttctgcga
     9901 tggagtaatc ggtgtctgca ttcatgtgtt tgtttttaat caggtcaacc aggaaggtca
     9961 gacccgcttt cgcgccagcg ttatccacgc ccacgtcttt aatgtcgtat ttgccatttt
    10021 catacttgaa cgcataaccc ccgtcagcag caatcagcgg ccaggtgaag tacggttctt
    10081 gcaggttgaa catcagcgcg ctcttacctt tcgctttcag ttctttatcc agcgccggga
    10141 tctcttccca ggtttttggt gggttcggca gcagatcttt gttataaatc agcgataacg
    10201 cttcaacagc gatcgggtaa gcaatcagct tgccgttgta acgtacggca tcccaggtaa
    10261 acggatacag cttgtcctgg aacgctttgt ccggggtgat ttcagccaac aggccagatt
    10321 gagcgtagcc accaaaacgg tcgtgcgccc agaagataat gtcagggcca tcgccagttg
    10381 ccgcaacctg agggaatttc tcttccagtt tatccggatg ctcaacggtg actttaattc
    10441 cggtatcttt ctcgaatttc ttaccgactt cagcgagacc gttatagcct ttatcgccgt
    10501 taatccagat taccagttta ccttcttcga ttttggcgag agccgaggcg gaaaacatca
    10561 tcgtcgttaa tgcggataat gcgaggatgc gtgcacctgt ttttattttc ataatctatg
    10621 gtccttgttg gtgaagtgct cgtgaaaaca cctaaacgga ctctagtttc tttatacggc
    10681 aacctctttc catcctcctt gcccctacgc cccaccgttg ctttgtgtga tctctgttac
    10741 agaattggcg gtaatgtgga gatgcgcaca taaaatcgcc acgatttttg caagcaacat
    10801 cacgaaattc cttacatgac ctcggtttag ttcacagaag ccgtgttctc atcctcccgc
    10861 ctcctccccc ataaaaaagc cagggggtgg aggatttaag ccatctcctg atgacgcata
    10921 gtcagcccat catgaatgtt gctgtcgatg acaggttgtt acaaagggag aagggcatgg
    10981 cgagcgtaca gctgcaaaat gtaacgaaag cctggggcga ggtcgtggta tcgaaagata
    11041 tcaatctcga tatccatgaa ggtgaattcg tggtgtttgt cggaccgtct ggctgcggta
    11101 aatcgacttt actgcgcatg attgccgggc ttgagacgat caccagcggc gacctgttca
    11161 tcggtgagaa acggatgaat gacactccgc cagcagaacg cggcgttggt atggtgtttc
    11221 agtcttacgc gctctatccc cacctgtcag tagcagaaaa catgtcattt ggcctgaaac
    11281 tggctggcgc aaaaaaagag gtgattaacc aacgcgttaa ccaggtggcg gaagtgctgc
    11341 aactggcgca tttgctggat cgcaaaccga aagcgctctc tggcggtcag cgtcagcgtg
    11401 tggcgattgg ccgtacgctg gtggccgagc caagcgtatt tttgctcgat gaaccgctct
    11461 ccaacctcga tgctgcactg cgtgtgcaaa tgcgtatcga aatctcccgt ctgcataaac
    11521 gcctgggccg cacaatgatt tacgtcaccc acgatcaggt cgaagcgatg acgctggccg
    11581 acaaaatcgt ggtgctggac gcaggtcgcg tggcgcaggt tgggaaaccg ctggagctgt
    11641 accactatcc ggcagaccgt tttgtcgccg gatttatcgg ttcgccaaag atgaacttcc
    11701 tgccggtaaa agtgaccgcc accgcaatcg atcaagtgca ggtggagctg ccgatgccaa
    11761 atcgtcagca agtctggctg ccagttgaaa gccgtgatgt ccaggttgga gccaatatgt
    11821 cgctgggtat tcgcccggaa catctactgc cgagtgatat cgctgacgtc atccttgagg
    11881 gtgaagttca ggtcgtcgag caactcggca acgaaaccca aatccatatc cagatccctt
    11941 ccattcgtca aaacctggtg taccgccaga acgacgtggt gttggtagaa gaaggtgcca
    12001 cattcgctat cggcctgccg ccagagcgtt gccatctgtt ccgtgaggat ggcactgcat
    12061 gtcgtcgact gcataaggag ccgggcgttt aagcacccca caaaacacac aaagcctgtc
    12121 acaggtgatg tgaaaaaaga aaagcaatga ctcaggagat agaatgatga ttactctgcg
    12181 caaacttcct ctggcggttg ccgtcgcagc gggcgtaatg tctgctcagg caatggctgt
    12241 tgatttccac ggctatgcac gttccggtat tggctggaca ggtagcggcg gtgaacaaca
    12301 gtgtttccag actaccggtg ctcaaagtaa ataccgtctt ggcaacgaat gtgaaactta
    12361 tgctgaatta aaattgggtc aggaagtgtg gaaagagggc gataagagct tctatttcga
    12421 cactaacgtg gcctattccg tcgcgcaaca gaatgactgg gaagctaccg atccggcctt
    12481 ccgtgaagca aacgtgcagg gtaaaaacct gatcgaatgg ctgccaggtt ccaccatctg
    12541 ggcaggtaag cgcttctacc aacgtcatga cgttcatatg atcgacttct actactggga
    12601 tatttctggt cctggtgccg gtctggaaaa catcgatgtt ggcttcggta aactctctct
    12661 ggcagcaacc cgctcctctg aagctggtgg ttcttcctct tttgccagca acaatattta
    12721 tgactatacc aacgaaaccg cgaacgacgt tttcgatgtg cgtttagcgc agatggaaat
    12781 caacccgggc ggcacattag aactgggtgt cgactacggt cgtgccaacc tgcgtgataa
    12841 ctatcgtctg gttgatggcg catcgaaaga cggctggtta ttcactgctg aacatactca
    12901 gagtgtcctg aagggcttta acaagtttgt tgttcagtac gctactgact cgatgacctc
    12961 gcagggtaaa ggtctgtcgc agggttctgg cgtcgcgttt gataacgaaa aatttgccta
    13021 caatatcaac aacaacggtc acatgctgcg tatcctcgac cacggtgcga tctccatggg
    13081 cgacaactgg gacatgatgt acgtgggtat gtaccaggat atcaactggg ataacgacaa
    13141 cggcaccaag tggtggaccg ttggtattcg cccgatgtac aagtggacgc caatcatgag
    13201 caccgtgatg gaaatcggct acgacaacgt cgaatcccag cgcaccggcg acaagaacaa
    13261 tcagtacaaa attaccctcg cacaacaatg gcaggctggc gacagcatct ggtcacgccc
    13321 ggctattcgt gtcttcgcaa cctacgccaa gtgggatgag aaatggggtt atgactacaa
    13381 cggcgatagc aaggttaacc cgaactacgg caaagccgtt cctgctgatt tcaacggcgg
    13441 cagcttcggt cgtggcgaca gcgacgagtg gaccttcggt gcccagatgg aaatctggtg
    13501 gtaatagcaa aacctgggcc ggataaggcg tttacgccgc attcggcaac caacgcctga
    13561 tgcgacgctt gcgcgtctta tcaggccgac aacggctgtc aaatgtaggc cggataaggc
    13621 gtttacgccg catccggcat aaaaacaggt tgtcattatc tgaaaggggc gaaagcccct
    13681 ctgattatcg ggtttagcgc gctattgcct ggctaccgct gagctccaga ttttgaggtg
    13741 aaaacaatga aaatgaataa aagtctcatc gccctctgtt tatcagcagg gttactggca
    13801 agcgcgcctg gaatcagcct tgccgatgtt aactacgtac cgcaaaacac cagcgacgcg
    13861 ccagccattc catctgctgc gctgcaacaa ctcacctgga caccggtcga tcaatctaaa
    13921 acccagacca cccaactggc gaccggcggc caacaactga acgttcccgg catcagtggt
    13981 ccggttgctg cgtacagcgt cccggcaaac attggcgaac tgaccctgac gctgaccagc
    14041 gaagtgaaca aacaaaccag cgtttttgcg ccgaacgtgc tgattcttga tcagaacatg
    14101 accccatcag ccttcttccc cagcagttat ttcacctacc aggaaccagg cgtgatgagt
    14161 gcagatcggc tggaaggcgt tatgcgcctg acaccggcgt tggggcagca aaaactttat
    14221 gttctggtct ttaccacgga aaaagatctc cagcagacga cccaactgct cgacccggct
    14281 aaagcctatg ccaagggcgt cggtaactcg atcccggata tccccgatcc ggttgctcgt
    14341 cataccaccg atggcttact gaaactgaaa gtgaaaacga actccagctc cagcgtgttg
    14401 gtaggacctt tatttggttc ttccgctcca gctccggtta cggtaggtaa cacggcggca
    14461 ccagctgtgg ctgcacccgc tccggcaccg gtgaagaaaa gcgagccgat gctcaacgac
    14521 acggaaagtt attttaatac cgcgatcaaa aacgctgtcg cgaaaggtga tgttgataag
    14581 gcgttaaaac tgcttgatga agctgaacgc ctgggatcga catctgcccg ttccaccttt
    14641 atcagcagtg taaaaggcaa ggggtaatta cgccccacag tgctgatttt gcaacaactg
    14701 gtgcgtctcc tggcgcacct ttttttatgc ttccttcctg ggatatgagc gattttttat
    14761 agtaactcac ttcttcttca ctaagaatat ccattatctc aatgccttat cagagattct
    14821 tttcctttcg ccggtagtgt ctggacattc aggctacttt tccaggttat tttatttctg
    14881 ttatgcagag gttttatgat aagtcatatc ctaaattctg gcggcaataa ctctttgatg
//
LOCUS       DAZKHF020000101        14927 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2201S31241
            SAMN43267800-rid25106413.denovo.102, whole genome shotgun sequence.
ACCESSION   DAZKHF020000101 DAZKHF020000000
VERSION     DAZKHF020000101.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267800
            Sequence Read Archive: SRR30307263
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 14927)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14927)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 14927)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350581.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350581.2
            Genome Coverage       :: 151x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:21:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,652
            CDSs (total)                      :: 5,557
            Genes (coding)                    :: 5,320
            CDSs (with protein)               :: 5,320
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 237
            CDSs (without protein)            :: 237
            Pseudo Genes (ambiguous residues) :: 0 of 237
            Pseudo Genes (frameshifted)       :: 102 of 237
            Pseudo Genes (incomplete)         :: 130 of 237
            Pseudo Genes (internal stop)      :: 52 of 237
            Pseudo Genes (multiple problems)  :: 41 of 237
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14927
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267800-rid25106413.denovo.102"
                     /serotype="O 26:H11"
                     /isolate="2201S31241"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-01"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            complement(71..146)
                     /locus_tag="ACHOSC_RS12655"
                     /old_locus_tag="pgaptmp_002531"
     tRNA            complement(71..146)
                     /locus_tag="ACHOSC_RS12655"
                     /old_locus_tag="pgaptmp_002531"
                     /product="tRNA-Phe"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(111..113),aa:Phe,seq:gaa)
     gene            complement(252..959)
                     /gene="yqgA"
                     /locus_tag="ACHOSC_RS12660"
                     /old_locus_tag="pgaptmp_002532"
     CDS             complement(252..959)
                     /gene="yqgA"
                     /locus_tag="ACHOSC_RS12660"
                     /old_locus_tag="pgaptmp_002532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF554 domain-containing protein"
                     /protein_id="HGU4548456.1"
                     /translation="MVIGPFINASAVLLGGVLGALLSQRLPERIRVSMTSIFGLASLG
                     IGILLVVKCANLPAMVLATLLGALIGEICLLEKGVNTAVAKAQNLFRHSRKKPAHESF
                     IQNYVAIIVLFCASGTGIFGAMNEGMTGDPSILIAKSFLDFFTAMIFACSLGIAVSVI
                     SIPLLIIQLTLAWAAALILPLTTPSMMADFSAVGGLLLLATGLRICGIKMFPVVNMLP
                     ALLLAMPLSAAWTAWFA"
     gene            1112..1195
                     /gene="yqgH"
                     /locus_tag="ACHOSC_RS12665"
                     /old_locus_tag="pgaptmp_002533"
     CDS             1112..1195
                     /gene="yqgH"
                     /locus_tag="ACHOSC_RS12665"
                     /old_locus_tag="pgaptmp_002533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YqgH"
                     /protein_id="HGU4548457.1"
                     /translation="MHAISLHSLAYRRFARNVSLSMLLLMR"
     gene            1357..3492
                     /gene="speC"
                     /locus_tag="ACHOSC_RS12670"
                     /old_locus_tag="pgaptmp_002534"
     CDS             1357..3492
                     /gene="speC"
                     /locus_tag="ACHOSC_RS12670"
                     /old_locus_tag="pgaptmp_002534"
                     /EC_number="4.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311868.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine decarboxylase"
                     /protein_id="HGU4548458.1"
                     /translation="MKSMNIAASSELVSRLSSHRRVVALGDTDFTDVAAVVITAADSR
                     SGILALLKRTGFHLPVFLYSEHAVELPAGVTAVINGNEQQWLELESAACQYEENLLPP
                     FYDTLTQYVEMGNSTFACPGHQHGAFFKKHPAGRHFYDFFGENVFRADMCNADVKLGD
                     LLIHEGSAKDAQKFAAKVFHADKTYFVLNGTSAANKVVTNALLTRGDLVLFDRNNHKS
                     NHHGALIQAGATPVYLEASRNPFGFIGGIDAHCFNEEYLRQQIRDVAPEKAELPRPFR
                     LAIIQLGTYDGTVYNARQVIDTVGHLCDYILFDSAWVGYEQFIPMMADSSPLLLELNE
                     NDPGIFVTQSVHKQQAGFSQTSQIHKKDNHIRGQARFCPHKRLNNAFMLHASTSPFYP
                     LFAALDVNAKIHEGESGRRLWAECVELGIESRKAILARCKLFRPFIPPVVDGKLWQDY
                     PTSVLASDRRFFSFEPGAKWHGFEGYAADQYFVDPCKLLLTTPGINAETGEYSDFGVP
                     ATILAHYLRENGIVPEKCDLNSILFLLTPAESHEKLAQLVAMLAQFEQHIEDDSPLAE
                     VLPSVYNKYPVRYRDYTLRQLCQEMHDLYVSFDVKDLQKAMFRQQSFPSVVMNPQDAH
                     SAYIRGEVELVRIRDAEGRIAAEGALPYPPGVLCVVPGEVWGGAVQRYFLALEEGGNL
                     LPGFSPELQGVYSETDADGMKRLYGYVLK"
     gene            complement(3542..4798)
                     /gene="nupG"
                     /locus_tag="ACHOSC_RS12675"
                     /old_locus_tag="pgaptmp_002535"
     CDS             complement(3542..4798)
                     /gene="nupG"
                     /locus_tag="ACHOSC_RS12675"
                     /old_locus_tag="pgaptmp_002535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311867.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside permease NupG"
                     /protein_id="HGU4548459.1"
                     /translation="MNLKLQLKILSFLQFCLWGSWLTTLGSYMFVTLKFDGASIGAVY
                     SSLGIAAVFMPALLGIVADKWLSAKWVYAICHTIGAITLFMAAQVTTPEAMFLVILIN
                     SFAYMPTLGLINTISYYRLQNAGMDIVTDFPPIRIWGTIGFIMAMWVVSLSGFELSHM
                     QLYIGAALSAILVLFTLTLPHIPVAKQQANQSWTTLLGLDAFALFKNKRMAIFFIFSM
                     LLGAELQITNMFGNTFLHSFDKDPMFASSFIVQHASIIMSISQISETLFILTIPFFLS
                     RYGIKNVMMISIVAWILRFALFAYGDPTPFGTVLLVLSMIVYGCAFDFFNISGSVFVE
                     KEVSPAIRASAQGMFLMMTNGFGCILGGIVSGKVVEMYTQNGITDWQTVWLIFAGYSV
                     VLAFAFMAMFKYKHVRVPTGTQTVSH"
     gene            complement(5000..6079)
                     /gene="mltC"
                     /locus_tag="ACHOSC_RS12680"
                     /old_locus_tag="pgaptmp_002536"
     CDS             complement(5000..6079)
                     /gene="mltC"
                     /locus_tag="ACHOSC_RS12680"
                     /old_locus_tag="pgaptmp_002536"
                     /EC_number="4.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417438.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008933 - lytic transglycosylase activity
                     [Evidence IEA];
                     GO_process: GO:0000270 - peptidoglycan metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="membrane-bound lytic murein transglycosylase
                     MltC"
                     /protein_id="HGU4548460.1"
                     /translation="MKKYLALALIAPLLISCSTTKKGDTYNEAWVKDTNGFDILMGQF
                     AHNIENIWGFKEVVIAGPKDYVKYTDQYQTRSHINFDDGTITIETIAGTEPAAHLRRA
                     IIKTLLMGDDPSSVDLYSDVDDITISKEPFLYGQVVDNTGQPIRWEGRASNFADYLLK
                     NRLKSRSNGLRIIYSVTINMVPNHLDKRAHKYLGMVRQASRKYGVDESLILAIMQTES
                     SFNPYAVSRSDALGLMQVVQHTAGKDVFRSQGKSGTPSRSFLFDPASNIDTGTAYLAM
                     LNNVYLGGIDNPTSRRYAVITAYNGGAGSVLRVFSNDKIQAANIINTMTPGDVYQTLT
                     TRHPSAESRRYLYKVNTAQKSYRRR"
     gene            complement(6144..6419)
                     /gene="yggX"
                     /locus_tag="ACHOSC_RS12685"
                     /old_locus_tag="pgaptmp_002537"
     CDS             complement(6144..6419)
                     /gene="yggX"
                     /locus_tag="ACHOSC_RS12685"
                     /old_locus_tag="pgaptmp_002537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oxidative damage protection protein"
                     /protein_id="HGU4548461.1"
                     /translation="MSRTIFCTFLQREAEGQDFQLYPGELGKRIYNEISKEAWAQWQH
                     KQTMLINEKKLNMMNAEHRKLLEQEMVNFLFEGKEVHIEGYTPEDKK"
     gene            complement(6447..7499)
                     /gene="mutY"
                     /locus_tag="ACHOSC_RS12690"
                     /old_locus_tag="pgaptmp_002538"
     CDS             complement(6447..7499)
                     /gene="mutY"
                     /locus_tag="ACHOSC_RS12690"
                     /old_locus_tag="pgaptmp_002538"
                     /EC_number="3.2.2.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019104 - DNA N-glycosylase activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="A/G-specific adenine glycosylase"
                     /protein_id="HGU4548462.1"
                     /translation="MQASQFSAQVLDWYDKYGRKTLPWQIDKTPYKVWLSEVMLQQTQ
                     VATVIPYFERFMARFPTVTDLANAPLDEVLHLWTGLGYYARARNLHKAAQQVATLHGG
                     KFPETFEEVAALPGVGRSTAGAILSLSLGKHFPILDGNVKRVLARCYAVSGWPGKKEV
                     ENKLWSLSEQVTPAVGVERFNQAMMDLGAMICTRSKPKCSLCPLQNGCIAAANNSWAL
                     YPGKKPKQTLPERTGYFLLLQHEDEVLLAQRPPSGLWGGLYCFPQFADEESLRQWLAQ
                     RQIAADNLTQLTAFRHTFSHFHLDIVPMWLPVSSFTGCMDEGNALWYNLAQPPSVGLA
                     APVERLLQQLRTGAPV"
     gene            7660..8379
                     /gene="trmB"
                     /locus_tag="ACHOSC_RS12695"
                     /old_locus_tag="pgaptmp_002539"
     CDS             7660..8379
                     /gene="trmB"
                     /locus_tag="ACHOSC_RS12695"
                     /old_locus_tag="pgaptmp_002539"
                     /EC_number="2.1.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008176 - tRNA
                     (guanine(46)-N7)-methyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(46)-N7)-methyltransferase TrmB"
                     /protein_id="HGU4548463.1"
                     /translation="MKNDVISPEFDENGRPLRRIRSFVRRQGRLTKGQEHALENYWPV
                     MGVEFSEDMLDFPALFGREAPVTLEIGFGMGASLVAMAKDRPEQDFLGIEVHSPGVGA
                     CLASAHEEGLSNLRVMCHDAVEVLHKMIPDNSLRMVQLFFPDPWHKARHNKRRIVQVP
                     FAELVKSKLQLGGVFHMATDWEPYAEHMLEVMSSIDGYKNLSESNDYVPRPASRPVTK
                     FEQRGHRLGHGVWDLMFERVK"
     gene            8379..8705
                     /gene="yggL"
                     /locus_tag="ACHOSC_RS12700"
                     /old_locus_tag="pgaptmp_002540"
     CDS             8379..8705
                     /gene="yggL"
                     /locus_tag="ACHOSC_RS12700"
                     /old_locus_tag="pgaptmp_002540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417434.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YggL family protein"
                     /protein_id="HGU4548464.1"
                     /translation="MAKNRSRRLRKKMHIDEFQELGFSVAWRFPEGTSEEQIDKTVDD
                     FINEVIEPNKLAFDGSGYLAWEGLICMQEIGKCTEEHQAIVRKWLEERKLDEVRTSEL
                     FDVWWD"
     gene            8889..9608
                     /gene="yggN"
                     /locus_tag="ACHOSC_RS12705"
                     /old_locus_tag="pgaptmp_002541"
     CDS             8889..9608
                     /gene="yggN"
                     /locus_tag="ACHOSC_RS12705"
                     /old_locus_tag="pgaptmp_002541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2884 domain-containing protein"
                     /protein_id="HGU4548465.1"
                     /translation="MMRKMLLAAALSVTAMTAHADYQCSVTPRDDVIVSPQTVQVKGE
                     NGNLVITPDGNVMYNGKQYSLNAAQREQAKDYQAELRSTLPWIDEGAKSRVEKARIAL
                     DKIIVQEMGESSKMRSRLTKLDAQLKEQMNRIIETRSDGLTFHYKAIDQVRAEGQQLV
                     NQAMGGILQDSINEMGAKAVLKSGGNPLQNVLGSLGGLQSSIQTEWKKQEKDFQQFGK
                     DVCSRVVTLEDSRKALVGNLK"
     gene            9784..10830
                     /gene="ansB"
                     /locus_tag="ACHOSC_RS12710"
                     /old_locus_tag="pgaptmp_002542"
     CDS             9784..10830
                     /gene="ansB"
                     /locus_tag="ACHOSC_RS12710"
                     /old_locus_tag="pgaptmp_002542"
                     /EC_number="3.5.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004067 - asparaginase activity [Evidence
                     IEA];
                     GO_process: GO:0006528 - asparagine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-asparaginase 2"
                     /protein_id="HGU4548466.1"
                     /translation="MEFFKKTALAALVMGFSGAALALPNITILATGGTIAGGGDSATK
                     SNYTAGKVGVENLVNAVPQLKDIANVKGEQVVNIGSQDMNDNVWLTLAKKINTDCDKT
                     DGFVITHGTDTMEETAYFLDLTVKCDKPVVMVGAMRPSTSMSADGPFNLYNAVVAAAD
                     KASANRGVLVVMNDTVLDGRDVTKTNTTDVATFKSVNYGPLGYIHNGKIDYQRTPARK
                     HTSDTPFDVSKLNELPKVGIVYNYANASDLPAKALVDAGYDGIVSAGVGNGNLYKSVF
                     DTLATAAKNGTAVVRSSRVPTGATTQDAEVDDAKYGFVASGTLNPQKARVLLQLALTQ
                     TKDPQQIQQIFNQY"
     gene            10947..11954
                     /gene="yggM"
                     /locus_tag="ACHOSC_RS12715"
                     /old_locus_tag="pgaptmp_002543"
     CDS             10947..11954
                     /gene="yggM"
                     /locus_tag="ACHOSC_RS12715"
                     /old_locus_tag="pgaptmp_002543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1202 family protein"
                     /protein_id="HGU4548467.1"
                     /translation="MKKQWIVGTALLMLMTGNAWADGEPPTENILKDQFKKQYHGILK
                     LDAITLKNLDAKGNQATWSAEGDVSSSDDLYTWVGQLADYELLEQTWTKDKPVKFSAM
                     LTSKGTPASGWSVNFYSFQAAASDRGRVVDDIKTNNKYLIVNSEDFNYRFSQLESALN
                     TQKNSIPALEKEVKALDKQMVAAQKAADAYWGKDANGKQMTREEAFKKIHQQRDEFNK
                     QNDSEAFAVKYDKEVYQPAIAACHKQSEECYEVPIQQKRDFDINEQRRQTFLQSQKLS
                     RKLQDDWVTLEKGQYPLTMKVSEINSKKVAILMKIDDINQANERWKKDTEQLRRNGVI
                     K"
     gene            complement(12010..13311)
                     /locus_tag="ACHOSC_RS12720"
                     /old_locus_tag="pgaptmp_002544"
     CDS             complement(12010..13311)
                     /locus_tag="ACHOSC_RS12720"
                     /old_locus_tag="pgaptmp_002544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708726.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0031317 - tripartite ATP-independent
                     periplasmic transporter complex [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter large permease"
                     /protein_id="HGU4548468.1"
                     /translation="MDSYIAITLFGCFFVLVFIGVPISFSIGIATVASMLLMFPWDIA
                     AITVSQRLANGLDNFALLAIPFFIFAGTLMNSGGIAIRLINLAQVMVGRVPGSLGHVN
                     VLANMMFGSISGSAVAAAAAVGGTLNPIQTKQGYDPAFSTAVNVSSCITGLLIPPSNV
                     LIVFSLTAGGVSVASLFMAGYLPGILMGLAVMIVCGIIAKRRGYPLSERATFAQACKA
                     FLDALPSLLLVFIVMGGILGGIFTATEASAIAVVYTFILSVLVYREVKWRHLPKLILE
                     SVVTTSIVLLLIGFSVGMSWAMTNADIPYMISDALMGISDNPLIILLLINIVLLIVGI
                     FMDMTPAVLIFTPIFLPIAQELGMDPVHFGIMMVANLCIGLLTPPVGSALFVGCSISG
                     VKIQHLIKPLLPFYAALLVALMMITYIPQISLFIPQLLGLM"
     gene            complement(13311..13814)
                     /locus_tag="ACHOSC_RS12725"
                     /old_locus_tag="pgaptmp_002545"
     CDS             complement(13311..13814)
                     /locus_tag="ACHOSC_RS12725"
                     /old_locus_tag="pgaptmp_002545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708723.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter small permease"
                     /protein_id="HGU4548469.1"
                     /translation="MIFNRLKLAVDRVIAAFSVAVMLALVVCVVWQVFSRYVLNQPST
                     LTDELARFLMIWVGLLGAAYTVGAQRHLSIDLFALALNKRKQLLLSIVINVLILGFAG
                     SVIVTGGLKLIDKTLATSQVSAAMQIPMGYVYIILPLSGLVMMFYALCFINQSIQQLK
                     QHAQEAS"
     gene            complement(13859..14845)
                     /locus_tag="ACHOSC_RS12730"
                     /old_locus_tag="pgaptmp_002546"
     CDS             complement(13859..14845)
                     /locus_tag="ACHOSC_RS12730"
                     /old_locus_tag="pgaptmp_002546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016231167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter substrate-binding protein"
                     /protein_id="HGU4548470.1"
                     /translation="MKMRSFTRSLVCASLLALVSTGVNAAEKVTLKLAHNLERSHVVH
                     QSFEELAKEVKQLSKGNMVIRIYPSSQMGNARETMELLQNGALDMTKGSASDLESFDN
                     IYAIYNLPFLFKDQAHFNKVVFGEVGKEIMDSTKDKGFFALSAYVAGTRSFYAKKPIT
                     KPEDLKGLKIRVQPSPTTIKMIELMGGSPTPISFGEVYTAMQQGVVDGAENNVPSWVQ
                     TRHIEIAKVFSEDEHASIPDFLVISTKTWNKLTPEQQQILETAAKKSEAYQQKLWEKI
                     DADTRAQAKAMGGEIVKVDKAPFRAAVQPLFDDFKKDPKQAALLEKFDNAAQ"
BASE COUNT         3956 a         3579 c         3776 g         3616 t
ORIGIN      
        1 ggcaggacgc ttaaatcgtg gcgtcctgaa acgaaaaacg gacctccgtg gaggtccgtt
       61 tatatgaatt tggtgcccgg actcggaatc gaaccaagga cacggggatt ttcaatcccc
      121 tgctctaccg actgagctat ccgggcaacg gggcgcatta aacctgattc gcctcgtctc
      181 gtcaatcaaa tttcttcaat ttactgcaga ctgcacaatc tatcatcact ttgccgctat
      241 tgcacgcatt gtcaggcaaa ccaggcggtc caggcggcgg aaagcggcat tgccagaagg
      301 agtgctggaa gcatgttgac caccgggaac attttaatgc cacagatgcg taatccggtt
      361 gccagcagca ataaaccgcc tacggcgctg aagtctgcca tcatcgacgg tgtggtcagc
      421 ggtaatatca gcgcggcagc ccacgccagc gttaactgga tgatcagtaa tgggatacta
      481 attaccgaca ccgcaatacc cagcgagcag gcgaagatca tcgccgtaaa gaaatcaaga
      541 aatgacttgg cgattaaaat actcggatcg ccggtcatcc cttcgttcat cgccccgaag
      601 atcccggtgc cgctggcgca aaacaggaca ataatcgcga cataattctg aataaaagat
      661 tcatgcgctg gcttcttacg tgagtgacga aacagatttt gtgctttggc gactgctgta
      721 ttgacacctt tctccagcaa acaaatttcg ccaattagag cgccaagtag cgtagctaaa
      781 accatcgctg gaaggttggc gcatttcaca accagtaaaa taccaatccc cagcgatgcc
      841 agaccaaaaa tagatgtcat ggagactcgg atacgttccg gtaagcgttg gctgagcagt
      901 gcgccgagaa cgccacccag taagactgca ctagcgttga taaaagggcc gatgaccaca
      961 agagttcctg ttagctgtat tgtttgattt tcttattatg aactttttgc cttgttgatg
     1021 gcttgtctgg tcgcaaagtc ctggcttgca cgctttagcg aaaggtgcca tgattgcgcg
     1081 aattttctcc tctctgtacg gagtttgccc gatgcacgcc atctccttac attctctcgc
     1141 ttatcgccgt ttcgcgcgaa acgtttccct ttctatgtta ctgctcatgc ggtgaagtta
     1201 acgcacgctc actgcaggac aacagtaaaa tcagagcgtt tctgctttta ctgatgtctg
     1261 gcggtcggag ctggtgacca gtttgaccca tatctcatgg ggcagggttt tccaccttgt
     1321 ccggtattct tacttccccg aaacgggttt gcgcttatga aatcaatgaa tattgccgcc
     1381 agtagtgaac tggtatcccg actttcttct catcgtcgcg tggtggcgtt gggagatact
     1441 gattttacgg acgtcgcggc agtcgtcatt accgctgcgg atagtcgcag tggcattctt
     1501 gcgttgctta agcgcaccgg ttttcatcta ccggtgtttt tgtattccga acatgctgtt
     1561 gaattacctg cgggcgttac ggcggtaatc aacggcaacg agcagcagtg gctggagctg
     1621 gaatccgcag cctgtcagta tgaagagaat ttgctgccac cgttttatga cacgctgacg
     1681 cagtacgttg agatgggcaa cagcaccttt gcttgccctg gacatcaaca tggtgcgttt
     1741 tttaaaaagc atcctgccgg acgccatttt tacgatttct ttggtgagaa cgtctttcgc
     1801 gccgatatgt gtaacgctga cgtaaaattg ggcgatctgc ttattcatga aggatcggcg
     1861 aaagatgcgc agaaattcgc agccaaagtc tttcatgccg ataaaaccta ttttgtgctg
     1921 aacggcacat cggcagcgaa taaagtggtg acgaatgcgc tgttaacgcg tggcgatctg
     1981 gtgctcttcg accgtaacaa ccataagtcg aatcatcacg gcgcgctgat tcaggcgggg
     2041 gcgacgccgg tctatctgga agcttcacgc aacccgtttg gtttcattgg cggtattgat
     2101 gcgcactgtt ttaatgaaga gtatctgcgc cagcaaattc gcgacgttgc gccagaaaaa
     2161 gccgagctgc cgcgcccgtt tcgcctggcg attattcagc tgggaaccta tgacggaact
     2221 gtctataacg cccgtcaggt gatcgatacc gttgggcatc tgtgtgatta cattctgttt
     2281 gattccgcgt gggtcggtta cgaacaattt atcccgatga tggcggatag ctcgccgctg
     2341 ctgttagaac ttaacgaaaa cgatccgggg atttttgtga cccagtcggt gcacaaacag
     2401 caggctggat tctcacagac gtcgcagatc cataaaaaag ataaccatat ccgcggacag
     2461 gcgcgttttt gcccgcataa gcggttgaat aacgccttta tgctccatgc ttctaccagc
     2521 cctttctatc cgctgtttgc tgcactggat gttaacgcca aaattcatga aggggagagt
     2581 gggcgtcggc tgtgggctga gtgcgttgag ttgggtattg agtcgcgcaa ggctattctt
     2641 gcgcgctgta agctgttccg cccgtttatc ccgcccgttg ttgatggcaa attgtggcag
     2701 gattatccga catctgtgtt agccagcgac cgccgttttt tcagttttga gccgggggcg
     2761 aagtggcacg gctttgaagg atatgccgcg gatcagtatt ttgttgatcc gtgcaagctg
     2821 ttactcacta caccaggtat caatgccgaa actggcgaat atagcgactt tggcgttccg
     2881 gcgacgattc tggcgcacta tctgcgtgag aacggcattg tgccggagaa gtgcgatctc
     2941 aactccattc tgttcttatt aactccggcg gaaagccacg agaagctggc acaactggtg
     3001 gcgatgctgg cgcaatttga acagcatatt gaggatgact cgccgctggc tgaggtgttg
     3061 ccgagcgttt ataacaaata tccggtgcgc tatcgcgact acaccctgcg ccagttgtgt
     3121 caggagatgc acgatctgta tgtcagtttc gacgtcaaag acctacaaaa agcgatgttc
     3181 cgccagcaga gtttcccgtc agtggtgatg aacccccagg atgcgcatag cgcttatatt
     3241 cgcggtgaag tggagttggt gcggattcgt gatgccgaag ggcgaattgc ggcagaaggg
     3301 gcgttgcctt atccacctgg cgtgctttgc gtggtacccg gggaagtctg gggtggggcg
     3361 gttcaacgtt atttccttgc actggaagaa ggggggaatt tgttgccggg attttcgccg
     3421 gagctgcaag gtgtttatag cgaaaccgat gcggatggca tgaaacggtt gtacggttat
     3481 gtgttgaagt aagaataaaa aaaacgggtc accttctggc gacccgtttt tctttgcgta
     3541 attagtggct aaccgtctgt gtgcctgtcg ggacacgaac gtgtttatat ttgaacatcg
     3601 ccatgaacgc gaaggccaga accacggagt aaccagcgaa aatcaaccat acggtctgcc
     3661 agtcggtaat gccgttttgg gtgtacatct caacaacttt accgctcacg atgccgccga
     3721 ggatacagcc gaagccgtta gtcatcatca ggaacatccc ttgtgcactg gcgcgaattg
     3781 ccgggctaac ttctttttcg acaaacaccg aaccagagat gttgaagaag tcgaatgcgc
     3841 aaccgtaaac gatcatcgac agtaccagca gtacagtacc gaacggagtc gggtcgccgt
     3901 aagcaaacag cgcaaaacgc aggatccacg ccacaatact gatcatcatt acgttcttaa
     3961 taccgtagcg gcttaagaag aacgggatgg tcagaatgaa cagggtttca gagatctgcg
     4021 aaatcgacat gatgattgac gcatgctgca caataaagct gctggcaaac atcggatctt
     4081 tgtcgaagct gtgcaggaag gtattaccga acatattggt aatctgcagt tccgcgccca
     4141 gcagcattga gaagataaag aagattgcca tacgcttgtt tttaaacagc gcgaatgcat
     4201 cgaggcccag cagggttgtc cagctctgat tcgcttgctg tttagcaacc ggaatatgcg
     4261 gcagagtcag ggtaaacaga accagaatgg cggaaagtgc tgcgccaata tacagctgca
     4321 tgtggcttaa ttcgaagcca gacaggctca ccacccacat tgccatgata aagccgatgg
     4381 tgccccagat acggattggc gggaagtcag taacgatatc catcccggca ttttgcaggc
     4441 gatagtaaga gatggtgttg attaacccaa gcgttggcat ataagcaaac gagttaatca
     4501 atatcacaag gaacatcgcc tccggtgtcg tgacctgtgc cgccatgaac agcgtgatag
     4561 cgccaatggt gtggcaaatg gcatataccc atttcgcact taaccatttg tcggccacaa
     4621 tccccagcag cgcaggcata aagaccgcgg cgatacccag tgagctataa actgcgccaa
     4681 tagaagcacc gtcaaacttc agggtaacaa acatatagga gccgagggtc gtcagccaac
     4741 ttccccacag acagaactgc agaaaagaga ggattttcag ctgcagctta agattcatgt
     4801 taatttcctc acatcgtgat gcggatgagt gtttgaaaaa gcacatttga ccgtgcgaga
     4861 tgatgggatt ctatcaactt cgcggactgg ttttttgtta cctgtggcaa ataattgcaa
     4921 acattcccaa tttgcaaaac gaaattgtga tgtggataac atttttgccc ctgagcatcg
     4981 tcaggggcgg ttaatggaat tatcggcggc ggtaggattt ttgcgcggta ttcactttat
     5041 aaagataacg gcgagattcc gcagagggat ggcgggtcgt cagcgtctga taaacatcgc
     5101 ccggcgtcat ggtgttaata atattggcag cctgaatctt atcattcgaa aagactcgca
     5161 gcacgctgcc tgcgccgccg ttataggcgg tgatgacggc ataacgccgc gatgttgggt
     5221 tatcaattcc gccgagataa acattgttca gcatcgccag atacgcggtg ccggtatcaa
     5281 tattgctggc aggatcaaac aagaaactgc ggctcggcgt gccggatttc ccctgcgagc
     5341 ggaacacatc tttcccggca gtatgttgta ccacctgcat taatcccagc gcatcggaac
     5401 ggctgaccgc atatgggtta aaggaagatt cggtctgcat aatcgccaga atcagcgact
     5461 catcaacgcc atatttccgt gacgcctggc ggaccatgcc gagatatttg tgcgcacgtt
     5521 tatcaaggtg gttcggcacc atgttaatgg taacgctgta gatgatacgc agcccgttgc
     5581 tgcggctctt cagacggttt ttcagcagat aatccgcgaa gttgctcgcg cgaccttccc
     5641 agcgaatcgg ctgcccggtg ttgtccacca cctgaccgta aaggaaaggt tctttcgaaa
     5701 tcgtaatatc atcaacgtcg gaatagagat cgaccgaact cggatcgtca cccatcagca
     5761 acgttttgat aattgcccgg cgcaaatgcg cggcaggttc tgtcccggcg atagtttcga
     5821 tagtaatcgt accgtcatcg aagttgatgt ggctgcgggt ctgatattga tcggtgtatt
     5881 tcacgtagtc cttaggaccg gcgatcacca cctctttgaa tccccagatg ttctcaatat
     5941 tgtgggcaaa ttgccccatc agaatatcaa aaccgttggt atctttgacc caggcttcgt
     6001 tataggtatc gccttttttg gtcgtcgaac aggagatgag caacggcgca atcaaagcca
     6061 gcgcgagata ttttttcatc attccgggtg cgtgttgtgt ttgtttttat gcaagttgcc
     6121 ggaggcgtgc tccggcactg tttttatttt ttatcttccg gcgtatagcc ctcgatatgc
     6181 acctctttac cctcgaacag gaagttgacc atctcctgct ccagcagctt gcggtgctcg
     6241 gcattcatca tgttgagttt cttttcatta atcagcatgg tttgtttgtg ctgccactgc
     6301 gcccaggctt ctttggagat ctcgttatag atgcgttttc ccagctcgcc ggggtacagc
     6361 tgaaaatcct gaccttctgc ttcacgttgc aggaaagtac aaaaaatcgt tctgctcata
     6421 aatcatcctc tttatcgact cacgcgctaa accggcgcgc cagtgcgtaa ctgctgtaac
     6481 aaacgctcca cgggagccgc caggccaact gacggcggtt gcgctaagtt ataccagagc
     6541 gcattgcctt catccatgca gccggtgaat gacgacacgg gaagccacat aggcacaata
     6601 tctaagtgga aatggctgaa ggtatgccga aacgcggtca gctgcgtcag gttatcggca
     6661 gcaatctgcc gttgcgccag ccactgccgc aaactttctt cgtcggcaaa ctgcgggaaa
     6721 cagtataaac cgccccacaa tccgctcggc ggacgctgcg ccagcaatac ttcatcttcg
     6781 tgctgtaata gcaaaaagta gccggtgcgc tccggcagcg tctgtttcgg ttttttgccc
     6841 ggataaagcg cccagctatt gttggcggcg gcaatacatc cgttttgtag cggacagagc
     6901 gaacatttcg gtttcgagcg cgtacaaatc atcgcaccca aatccatcat cgcctgatta
     6961 aaccgttcca cgccaaccgc gggcgtcacc tgctcgctca aactccataa tttattctcg
     7021 acctcttttt tccctggcca gccgcttaca gcatagcagc gcgccagcac ccgtttgacg
     7081 ttaccgtcga gaatcggaaa gtgcttaccc agagaaagcg agagaatcgc acctgcggtg
     7141 gaacgcccga cgcccggtaa cgccgcgact tcttcaaagg tttccgggaa tttaccgccg
     7201 tgtaaggtcg ccacttgttg tgccgcttta tgcaggttgc gcgcgcgggc gtaatagcca
     7261 agcccggtcc acaagtggag aacttcgtcg agcggcgcat tggcgagatc ggtcaccgtc
     7321 gggaagcgcg ccataaagcg ttcaaaatag gggataacgg tcgcaacctg agtttgttgc
     7381 aacatcactt ctgagagcca tactttgtag ggcgtcttgt caatttgcca gggcagcgtt
     7441 ttccgcccgt atttatcgta ccagtccaga acctgggctg aaaattgcga cgcttgcatg
     7501 gtcaccgaat tcactgttgt tgggggcaag attgcagcac agcgacggca gggtgtaaac
     7561 cggaactttc cgcagccacg gccttatcat gcttgcatcc ggcaactaac tttggataat
     7621 gcccgttttc agaacacttt cacaagcgac taaatcttta tgaaaaacga cgtcatttca
     7681 ccggaatttg atgaaaacgg ccgcccactg cgccgtatcc gtagttttgt gcgccgccag
     7741 gggcgactga ccaaaggcca ggaacatgcg ctggaaaact actggccggt gatgggcgtt
     7801 gagttcagcg aagatatgct ggatttcccc gcgctttttg gccgtgaagc gccggtgacg
     7861 cttgagattg gttttggcat gggggcgtcg ctggtggcaa tggctaaaga tcgccctgag
     7921 caggacttcc tcggcattga agtgcattca ccgggcgttg gtgcgtgcct ggcttctgcg
     7981 catgaagaag gtttaagcaa cctgcgcgtg atgtgtcacg atgcggttga agtgctgcat
     8041 aaaatgattc ctgacaattc attgcgcatg gtgcagctct ttttccctga cccgtggcac
     8101 aaagcgcgcc ataataaacg ccgtatcgta caggtgccgt ttgccgaact ggtaaaaagc
     8161 aaactgcagc tggggggcgt attccatatg gcgaccgact gggaacctta tgcggaacat
     8221 atgcttgaag tgatgtcttc tattgacggt tataaaaacc tgtcagagag caatgattac
     8281 gtaccgcgtc cggcatcacg tccggtgacg aaatttgaac aacgtggtca tcgtcttggt
     8341 cacggagtat gggacttaat gttcgagagg gtgaaataat ggcaaagaac cgtagccgtc
     8401 gtctgcgtaa aaaaatgcac atcgacgaat tccaggaatt aggattttcg gtggcatggc
     8461 gattcccgga aggtacatcg gaagaacaga ttgataaaac cgttgatgat tttattaacg
     8521 aggttatcga accgaacaaa ctggcctttg acggcagcgg ttatctggcc tgggaaggtc
     8581 tgatctgcat gcaggaaatc ggcaaatgca ccgaagaaca tcaggcgatt gtgcgtaagt
     8641 ggctggaaga gcgcaaactg gatgaggtac gcaccagcga acttttcgac gtttggtggg
     8701 actaagaaag catacgggcg atgacaaatg caaaactgcc tgatgcgcta cgcttatcag
     8761 gcctggaaag atgcacgatc gagtaggcgg gataaggtgt ttacgccgca tccggcatgg
     8821 aaaacgcgta ctttgttatc aatctggggc cagcaaatgc tggcctgatt ttttcttgag
     8881 ggaagactat gatgcgcaaa atgctgctgg cggcagcact ttcagtgacg gcaatgaccg
     8941 ctcacgccga ctaccagtgc agcgtcacgc cgcgtgacga tgtgattgtc agcccgcaaa
     9001 ccgtgcaggt gaagggcgaa aacggcaatc tggtgatcac gccagacggc aacgtgatgt
     9061 ataacggtaa gcaatattcc ctgaatgccg cccagcgcga gcaggcgaag gattatcagg
     9121 ctgaactacg cagcacgctg ccgtggattg atgaaggcgc gaaaagccgc gtcgaaaaag
     9181 cccgtattgc gctggataaa attatcgttc aggagatggg cgaaagcagc aaaatgcgca
     9241 gccgtctgac caaacttgat gcgcagctga aagagcagat gaaccgcatt atcgaaacgc
     9301 gcagcgatgg cctgacgttt cactataaag ccattgatca ggttcgcgcc gaaggccagc
     9361 aattagtgaa tcaggcaatg ggtggaattt tacaggacag cattaatgaa atgggcgcga
     9421 aagcggtgct gaaaagcggc ggtaacccat tacagaatgt gctgggaagc ctgggggggc
     9481 tgcaatcctc aatccaaacc gagtggaaaa agcaggaaaa agatttccag cagtttggca
     9541 aagatgtttg tagccgcgtt gtgactctgg aagatagccg caaagccctg gtcgggaatt
     9601 taaaataatc ctctatttta agacggcata atactttttt atgccgttta attcttcgtt
     9661 ttgttacctg cctctaactt tgtagatctc caaaatatat tcacgttgta aattgtttaa
     9721 cgtcaaattt cccatacaga gctaagggat aatgcgtagc gttcacgtaa ctggaggaat
     9781 gaaatggagt ttttcaaaaa gacggcactt gccgcactgg ttatgggttt tagtggtgca
     9841 gcattggcat tacccaatat caccatttta gcaaccggcg ggaccattgc cggtggtggt
     9901 gactccgcaa ccaaatctaa ctacacagcg ggtaaagttg gcgtagaaaa tctggttaat
     9961 gcggtgccgc aactaaaaga cattgcgaac gttaaaggcg agcaggtagt gaatatcggc
    10021 tcccaggaca tgaacgataa tgtctggctg acactggcga aaaaaattaa caccgactgc
    10081 gataaaaccg acggcttcgt cattacccac ggtaccgaca cgatggaaga aaccgcttac
    10141 ttcctcgacc tgacggtgaa atgcgacaaa ccggtggtga tggtcggcgc aatgcgcccg
    10201 tccacgtcca tgagcgcaga cggtccattc aacctgtata acgcggtagt ggccgcagct
    10261 gataaagcct ccgctaatcg tggcgtgctg gtggtgatga acgacaccgt actggacggt
    10321 cgcgatgtca ccaaaaccaa caccaccgac gtagcgacct tcaagtctgt taactacggt
    10381 cctctgggat acattcacaa cggtaagatt gactaccaac gtaccccggc acgtaagcac
    10441 accagcgata cgccattcga tgtctctaag ctgaatgagc tgccgaaagt cggcatcgtt
    10501 tataactacg ctaacgcatc cgatcttccg gctaaagcac tggtagatgc gggctatgat
    10561 ggcatcgtta gcgctggtgt gggtaatggt aacctgtata aatccgtgtt cgacaccctg
    10621 gcaaccgccg cgaaaaacgg cactgcagta gtgcgttctt cccgcgtacc gacgggtgct
    10681 accactcagg atgctgaagt ggatgatgcg aaatacggct tcgtcgcctc tggcacgctg
    10741 aacccgcaaa aagcgcgcgt cctgctgcag ctggctctga cgcaaaccaa agatccgcag
    10801 cagatccagc agatcttcaa tcagtactaa tcacctcgcc ccggtatcgt gccggggctt
    10861 tttcacttca gacttacgtc cattgccaat tttcattacc ctaatgataa tcaccggaat
    10921 aaattattcc gcgtgaggtt tttcgggtga aaaagcaatg gattgttggc acggcactgc
    10981 ttatgttgat gactggtaat gcctgggcag atggcgaacc gccaactgaa aatatcttaa
    11041 aagatcaatt caaaaagcag tatcacggca ttctcaagct tgatgctatc accttaaaaa
    11101 atcttgatgc taagggtaat caggccacct ggtcagcgga aggcgatgtc tcttccagtg
    11161 acgatctcta tacctgggtc ggtcagttgg cagattacga actgctcgaa cagacctgga
    11221 cgaaagataa accggtaaaa ttctcggcga tgttaaccag taaaggaacg cccgcgtctg
    11281 gctggtcggt gaacttttac tcttttcagg cggcagccag cgatcgtggg cgggtggtcg
    11341 acgatatcaa aacgaataat aaatatctga tcgtgaatag cgaagatttc aattatcgct
    11401 ttagtcagct tgaatctgcg ttgaataccc agaagaactc gattcctgcg ctggaaaagg
    11461 aagtgaaagc gcttgataag caaatggtgg cggcgcagaa agcagcggat gcatattggg
    11521 gaaaagatgc aaacggtaag caaatgaccc gcgaagaagc ctttaaaaaa attcaccaac
    11581 agcgtgatga gtttaataaa cagaacgata gcgaggcgtt cgcggttaaa tatgacaaag
    11641 aggtttatca accggcgatt gcggcatgtc ataaacagag tgaagagtgt tatgaagtgc
    11701 cgattcagca gaaacgagat ttcgatatca acgaacaacg gcgacagact tttctgcaat
    11761 cacaaaaact cagccgtaaa ttgcaggatg actgggtaac gcttgaaaaa gggcaatatc
    11821 cgttaacgat gaaagtttca gaaataaaca gtaaaaaagt cgcgattttg atgaaaattg
    11881 acgatatcaa ccaagctaat gaacgctgga aaaaagatac cgaacaactg cggcgcaatg
    11941 gggtgattaa gtaagtttga tatagtgcct ttgccggata atataaaaag ttatccggca
    12001 aattattttt tacattagcc ctagtagctg tggaatgaac aaggatattt gcggaatgta
    12061 agtgatcatc atcaacgcta ctagcaatgc agcataaaat ggcagcaatg gtttgattaa
    12121 atgttgaatc ttcacacccg agatagaaca accaacaaat agcgcactac ccaccggcgg
    12181 tgttaacagc ccaatacaaa ggttggccac catcataata ccgaaatgta ctgggtccat
    12241 tcccagctct tgtgcaatcg gcaagaagat cggtgtaaag attagtacag ccggtgtcat
    12301 gtccataaag atgccgacaa ttaataagac gatattaatc agcagcaata tgatcagtgg
    12361 gttatcagaa atccccatca atgcgtcgct gatcatataa ggaatatcag cattagtcat
    12421 tgcccaggac atgcccacgg aaaaaccgat caacagtaat acaatcgaag tggtaacaac
    12481 cgactccaga atcagtttgg gtaaatggcg ccattttact tcccggtaaa ccagcaccga
    12541 gagaataaat gtgtagacaa cggcaattgc tgacgcttca gttgcggtaa aaatgccgcc
    12601 cagaatgccg cccatcacaa tgaacaccag aagcaaactt ggtaaggcat ccagaaacgc
    12661 tttacaggcc tgagcaaagg ttgctctttc tgaaagcggg tagccacggc gtttagcaat
    12721 aatgccgcag actatcatta ccgcaagacc cattaagatc cctggcaaat atcccgccat
    12781 aaatagcgat gctacagaaa cgccgccagc tgtcagggaa aacacaatta acacgtttga
    12841 gggcggtatc agtaatccag taatacaaga tgagacgttt acagcggtag aaaatgccgg
    12901 atcataacct tgtttggtct gaatgggatt cagtgtgcca ccaacggcag cagctgccgc
    12961 aacggctgaa ccagaaatgg aaccaaacat catattcgcc aggacgttca catgaccaag
    13021 tgaaccagga acccgaccaa ccattacctg ggcgaggtta atcaaacgta tcgcaatacc
    13081 gccgctgttc atcagcgttc cggcaaagat gaaaaagggg attgccaata gggcaaagtt
    13141 atccagtcca ttagccagtc gctgcgaaac agtgattgcc gcgatatccc agggaaacat
    13201 caaaagcatt gaagctacag tggcaatacc gattgagaac gaaatgggca caccaataaa
    13261 gaccaacaca aaaaaacagc caaacagtgt aatcgcgata taactatcca ttaactggcc
    13321 tcctgcgcat gttgtttcag ttgttgaatg ctttgattaa taaaacacaa ggcataaaac
    13381 atcatcacca atccacttaa tggcagaatg atatatacgt atcccatcgg tatttgcatc
    13441 gcggcagata cctgtgatgt tgccagcgtt ttatcaatta atttcaggcc accagtcaca
    13501 atgaccgaac cggcaaaacc taaaataaga acgttaatga ctatactaag aagtaattgc
    13561 ttgcgcttat tcagggcgag agcaaataaa tcgatagaga gatgtctctg cgcgccgacg
    13621 gtatatgcag cacctaataa ccctacccag atcatcaaaa atcgtgccag ctcatcggtt
    13681 aatgtacttg gctggttaag tacgtagcgg ctaaatactt gccagactac gcatacaacc
    13741 agagcaagca tgacggcgac agaaaacgcg gcaatgacgc ggtctaccgc aagtttcagg
    13801 cggttaaata tcattgtaag catcctggaa aatcagggcc agagactggc ccggaatatt
    13861 attgagcagc gttgtcgaac ttctctaaca aagcagcttg ttttggatct tttttgaagt
    13921 cgtcaaacag aggctgaact gcggcacgga atggtgcttt atccactttt acaatttcac
    13981 cccccatagc tttcgcctga gcacgtgtgt cagcgtcaat tttttcccac aatttttgtt
    14041 gataagcttc ggatttcttc gctgctgttt cgaggatttg ttgctgttct ggggttaatt
    14101 tattccatgt tttggtggag ataaccaaaa agtcaggaat tgaggcgtgt tcatcttcag
    14161 aaaaaacttt ggcaatttca atatgtcgag tctgcaccca ggaaggcacg ttattttctg
    14221 cgccatctac tacaccttgc tgcatcgcag tataaacttc gccaaatgaa attggagtgg
    14281 gtgaaccacc catcagttca atcattttga tggtggtcgg acttggctga acacgaattt
    14341 tcagcccttt aagatcttct ggcttagtaa tcggtttttt cgcatagaaa ctgcgtgtac
    14401 ccgctacata agctgataaa gcaaaaaagc ctttgtcttt tgttgagtcc ataatttctt
    14461 taccaacttc accaaaaaca actttgttga agtgagcctg atctttaaat aagaacggca
    14521 aattatagat agcataaata ttatcaaaag actccagatc actggcggaa cctttggtca
    14581 tatccagagc gccattttgc aacaactcca tagtttctcg cgcattaccc atctggctgc
    14641 tgggataaat acggatcacc atattacctt ttgacagctg ttttacctct ttcgccagct
    14701 cttcaaaact ctgatgtaca acatgacttc tttccaggtt gtgtgctaac tttaacgtca
    14761 ctttttctgc ggcgtttacg ccagtgctaa ccagagccag caaggatgcg cagactaatg
    14821 agcgggtaaa tgacctcatt ttcataaaaa aactctctgt tgaaggatgg aaattggttg
    14881 atatctaatt gtgcttatca gtgctttgtg cgacaaattg gttaacc
//
LOCUS       DAZKHF020000102        14894 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2201S31241
            SAMN43267800-rid25106413.denovo.103, whole genome shotgun sequence.
ACCESSION   DAZKHF020000102 DAZKHF020000000
VERSION     DAZKHF020000102.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267800
            Sequence Read Archive: SRR30307263
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 14894)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14894)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 14894)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350581.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350581.2
            Genome Coverage       :: 151x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:21:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,652
            CDSs (total)                      :: 5,557
            Genes (coding)                    :: 5,320
            CDSs (with protein)               :: 5,320
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 237
            CDSs (without protein)            :: 237
            Pseudo Genes (ambiguous residues) :: 0 of 237
            Pseudo Genes (frameshifted)       :: 102 of 237
            Pseudo Genes (incomplete)         :: 130 of 237
            Pseudo Genes (internal stop)      :: 52 of 237
            Pseudo Genes (multiple problems)  :: 41 of 237
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14894
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267800-rid25106413.denovo.103"
                     /serotype="O 26:H11"
                     /isolate="2201S31241"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-01"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            <1..516
                     /locus_tag="ACHOSC_RS12735"
                     /old_locus_tag="pgaptmp_002547"
     CDS             <1..516
                     /locus_tag="ACHOSC_RS12735"
                     /old_locus_tag="pgaptmp_002547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001683249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HGU4548471.1"
                     /translation="PLSEVANLIKILQSCQGKVVFIGVGKSGIIARKLAATFASTGTP
                     SFFVHGTEAVHGDLGMVAKDDVVILISNSGETAEILATLPSLKKMGNYLISFTRSHHS
                     SLAISCDLSVEIPVKSEADNLGLAPSCSSTVVLVVGDAVALALSELKKFTRADFGLYH
                     PGGALGIKANS"
     gene            588..1535
                     /locus_tag="ACHOSC_RS12740"
                     /old_locus_tag="pgaptmp_002548"
     CDS             588..1535
                     /locus_tag="ACHOSC_RS12740"
                     /old_locus_tag="pgaptmp_002548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001361319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate dehydrogenase"
                     /protein_id="HGU4548472.1"
                     /translation="MKNVLVTVPSFSARCVSASKLLRENNFNLIIKNNVEHLLKSEST
                     ALRESICAVIAGKDCYQADTLSLLPGVKIISRFGTGIDNIDLRAAQQSGIVVNNAVGI
                     NSNAVAEFIIGLIFASMRNIPGSYHAMQNGYWGESHGYELQGKRIGMVGYGNIGKTLA
                     KRLSGFDVELLAYDKQPDYQVADKVGVQFVSIEDIFMQSHVIIVLLPFSPELENFISH
                     KYLSMMRNGALIINAARGKLLDEGALLQVIEERNVFAALDVFSSEPLAQFSPLLHAKN
                     IITTPHIAAATVESYQQTGTHVAQSIIDYFAGREIKNVL"
     gene            complement(1615..1691)
                     /locus_tag="ACHOSC_RS12745"
                     /old_locus_tag="pgaptmp_002549"
     tRNA            complement(1615..1691)
                     /locus_tag="ACHOSC_RS12745"
                     /old_locus_tag="pgaptmp_002549"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1655..1657),aa:Met,seq:cat)
     gene            1900..2997
                     /gene="mltA"
                     /locus_tag="ACHOSC_RS12750"
                     /old_locus_tag="pgaptmp_002550"
     CDS             1900..2997
                     /gene="mltA"
                     /locus_tag="ACHOSC_RS12750"
                     /old_locus_tag="pgaptmp_002550"
                     /EC_number="4.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008785702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA];
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0009254 - peptidoglycan turnover [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="murein transglycosylase A"
                     /protein_id="HGU4548473.1"
                     /translation="MKGRWVKYLLMGTVVAMLAACSSKPTDRGQQYKDGKFTQPFSLV
                     NQPDAVGAPINAGDFAEQINHIRNSSPRLYGNQSNVYNAVQEWLRAGGDTRNMRQFGI
                     DAWQMEGADNYGNVQFTGYYTPVIQARHTRQGEFQYPIYRMPPKRGRLPSRAEIYAGA
                     LSDKYILAYSNSLMDNFIMDVQGSGYIDFGDGSPLNFFSYAGKNGHAYRSIGKVLIDR
                     GEVKKEDMSMQAIRHWGETHSEAEVRELLEQNPSFVFFKPQSFAPVKGASAVPLVGRA
                     SVASDRSIIPPGTTLLAEVPLLDNNGKFNGQYELRLMVALDVGGAIKGQHFDIYQGIG
                     PEAGHRAGWYNHYGRVWVLKTAPGAGNVFSG"
     gene            3074..3880
                     /gene="tcdA"
                     /locus_tag="ACHOSC_RS12755"
                     /old_locus_tag="pgaptmp_002551"
     CDS             3074..3880
                     /gene="tcdA"
                     /locus_tag="ACHOSC_RS12755"
                     /old_locus_tag="pgaptmp_002551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008641 - ubiquitin-like modifier
                     activating enzyme activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA cyclic N6-threonylcarbamoyladenosine(37)
                     synthase TcdA"
                     /protein_id="HGU4548474.1"
                     /translation="MSVVISDAWRQRFGGTARLYGEKALQLFADAHICVVGIGGVGSW
                     AAEALARTGIGAITLIDMDDVCVTNTNRQIHALRDNVGLAKAEVMAERIRQINPECRV
                     TVVDDFVTPDNVAQYMSVGYSYVIDAIDSVRPKAALIAYCRRNKIPLVTTGGAGGQID
                     PTQIQVTDLAKTIQDPLAAKLRERLKSDFGVVKNSKGKLGVDCVFSTEALVYPQSDGT
                     VCAMKATAEGPKRMDCASGFGAATMVTATFGFVAVSHALKKMMAKAARQG"
     gene            complement(3931..4374)
                     /gene="csdE"
                     /locus_tag="ACHOSC_RS12760"
                     /old_locus_tag="pgaptmp_002552"
     CDS             complement(3931..4374)
                     /gene="csdE"
                     /locus_tag="ACHOSC_RS12760"
                     /old_locus_tag="pgaptmp_002552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase sulfur acceptor subunit
                     CsdE"
                     /protein_id="HGU4548475.1"
                     /translation="MTNPQFAGHPFGTTVTAETLRNTFAPLTQWEDKYRQLIMLGKQL
                     PALPDELKAQAKEIAGCENRVWLGYTVAENGKMHFFGDSEGRIVRGLLAVLLTAVEGK
                     TAAELQAQSPLALFDELGLRGQLSASRSQGLNALSEAIIAATKQV"
     gene            complement(4374..5579)
                     /gene="csdA"
                     /locus_tag="ACHOSC_RS12765"
                     /old_locus_tag="pgaptmp_002553"
     CDS             complement(4374..5579)
                     /gene="csdA"
                     /locus_tag="ACHOSC_RS12765"
                     /old_locus_tag="pgaptmp_002553"
                     /EC_number="3.13.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_function: GO:0031071 - cysteine desulfurase activity
                     [Evidence IEA];
                     GO_process: GO:0006534 - cysteine metabolic process
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase CsdA"
                     /protein_id="HGU4548476.1"
                     /translation="MNVFNPAQFRAQFPALQDAGVYLDSAATALKPEAVVEATRQFYS
                     LSAGNVHRSQFAEAQRLTARYEAAREKVAQLLNAPDDKTIVWTRGTTESINMVAQCYA
                     RPRLQLGDEIIVSVAEHHANLVPWLMVAQQTGAKVVKLPLNAQRLPDVDLLPELITPR
                     SRILALGQMSNVTGGCPDLARAITFAHSAGMVVMVDGAQGAVHFPADVQQLNIDFYAF
                     SGHKLYGPTGIGVLYGKSELLEAMSPWLGGGKMVHEVSFDGFTTQSAPWKLEAGTPNV
                     AGVIGLSAALEWLADYDINQAENWSRSLATLAEDALAKRPGFRSFRCQDSSLLAFDFA
                     GVHHSDMVTLLAEYGIALRAGQHCAQPLLAELGVTGTLRASFAPYNTKSDVDALVNAV
                     DRALELLVD"
     gene            5771..5998
                     /gene="ygdI"
                     /locus_tag="ACHOSC_RS12770"
                     /old_locus_tag="pgaptmp_002554"
     CDS             5771..5998
                     /gene="ygdI"
                     /locus_tag="ACHOSC_RS12770"
                     /old_locus_tag="pgaptmp_002554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417289.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YgdI/YgdR family lipoprotein"
                     /protein_id="HGU4548477.1"
                     /translation="MKKTAAIISACMLTFALSACSGSNYVMHTNDGRTIVSDGKPQTD
                     NDTGMISYKDANGNKQQINRTDVKEMVELDQ"
     gene            6349..7266
                     /gene="gcvA"
                     /locus_tag="ACHOSC_RS12775"
                     /old_locus_tag="pgaptmp_002555"
     CDS             6349..7266
                     /gene="gcvA"
                     /locus_tag="ACHOSC_RS12775"
                     /old_locus_tag="pgaptmp_002555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005130970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system transcriptional
                     regulator GcvA"
                     /protein_id="HGU4548478.1"
                     /translation="MSKRLPPLNALRVFDAAARHLSFTRAAEELFVTQAAVSHQIKSL
                     EDFLGLKLFRRRNRSLLLTEEGQSYFLDIKEIFSQLTEATRKLQARSAKGALTVSLLP
                     SFAIHWLVPRLSSFNSAYPGIDVRIQAVDRQEDKLADDVDVAIFYGRGNWPGLRVEKL
                     YAEYLLPVCSPLLLTGEKPLKTPEDLAKHTLLHDASRRDWQTYTRQLGLNHINVQQGP
                     IFSHSAMVLQAAIHGQGVALANNVMAQSEIEAGRLVCPFNDVLVSKNAFYLVCHDSQA
                     ELGKIAAFRQWILAKAAAEQEKFRFRYEQ"
     gene            7285..7680
                     /gene="ygdD"
                     /locus_tag="ACHOSC_RS12780"
                     /old_locus_tag="pgaptmp_002556"
     CDS             7285..7680
                     /gene="ygdD"
                     /locus_tag="ACHOSC_RS12780"
                     /old_locus_tag="pgaptmp_002556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF423 domain-containing protein"
                     /protein_id="HGU4548479.1"
                     /translation="MTSRFMLIFAAISGFIFVALGAFGAHVLSKTMGAVEMGWIQTGL
                     EYQAFHTLAILGLAVAMQRRISIWFYWSSVFLALGTVLFSGSLYCLALSHLRLWAFVT
                     PVGGVSFLAGWALMLVGAIRLKRKGVSHE"
     gene            7673..8773
                     /gene="rlmM"
                     /locus_tag="ACHOSC_RS12785"
                     /old_locus_tag="pgaptmp_002557"
     CDS             7673..8773
                     /gene="rlmM"
                     /locus_tag="ACHOSC_RS12785"
                     /old_locus_tag="pgaptmp_002557"
                     /EC_number="2.1.1.186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010435097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (cytidine(2498)-2'-O)-methyltransferase
                     RlmM"
                     /protein_id="HGU4548480.1"
                     /translation="MNKVVLLCRPGFEKECAAEITDKAGQREIFGFARVKENAGYVIY
                     ECYQPDDGDKLIRELPFSSLIFARQWFVVGELLQHLPPEDRITPIVGMLQGVVEKGGE
                     LRVEVADTNESKELLKFCRKFTVPLRAALRDAGVLANYETPKRPVVHVFFIAPGCCYT
                     GYSYSNNNSPFYMGIPRLKFPADAPSRSTLKLEEAFHVFIPADEWDERLANGMWAVDL
                     GACPGGWTYQLVKRNMWVYSVDNGPMAQSLMDTGQVTWLREDGFKFRPTRSNISWMVC
                     DMVEKPAKVAALMAQWLVNGWCRETIFNLKLPMKKRYEEVSHNLAYIQAQLDEHGINA
                     QIQARQLYHDREEVTVHVRRIWAAVGGRRDER"
     gene            complement(8817..9548)
                     /gene="fucR"
                     /locus_tag="ACHOSC_RS12790"
                     /old_locus_tag="pgaptmp_002558"
     CDS             complement(8817..9548)
                     /gene="fucR"
                     /locus_tag="ACHOSC_RS12790"
                     /old_locus_tag="pgaptmp_002558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-fucose operon activator"
                     /protein_id="HGU4548481.1"
                     /translation="MKAARQQAIVDLLLNHTSLTTEALSEQLKVSKETIRRDLNELQT
                     QGKILRNHGRAKYIHRQNQDSGDPFHIRLKSHYAHKADIAREALAWIEEGMVIALDAS
                     STCWYLARQLPDINIQVFTNSHPICHELGKRERIQLISSGGTLERKYGCYVNPSLISQ
                     LKSLEIDLFIFSCEGIDSSGALWDSNAINADYKSMLLKRAAQSLLLIDKSKFNRSGEA
                     RIGHLDEVTHIISDERQVATSLVTA"
     gene            complement(9606..10028)
                     /gene="fucU"
                     /locus_tag="ACHOSC_RS12795"
                     /old_locus_tag="pgaptmp_002559"
     CDS             complement(9606..10028)
                     /gene="fucU"
                     /locus_tag="ACHOSC_RS12795"
                     /old_locus_tag="pgaptmp_002559"
                     /EC_number="5.1.3.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016854 - racemase and epimerase activity
                     [Evidence IEA];
                     GO_function: GO:0042806 - fucose binding [Evidence IEA];
                     GO_process: GO:0042354 - L-fucose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-fucose mutarotase"
                     /protein_id="HGU4548482.1"
                     /translation="MLKTISPLISPELLKVLAEMGHGDEIIFSDAHFPAHSMGPQVIR
                     ADGLLVSDLLQAIIPLFELDSYAPPLVMMAAVEGDTLDPEVERRYRNALSLQAPCPDI
                     IRINRFAFYERAQKAFAIVITGERAKYGNILLKKGVTP"
     gene            complement(10030..11448)
                     /gene="fucK"
                     /locus_tag="ACHOSC_RS12800"
                     /old_locus_tag="pgaptmp_002560"
     CDS             complement(10030..11448)
                     /gene="fucK"
                     /locus_tag="ACHOSC_RS12800"
                     /old_locus_tag="pgaptmp_002560"
                     /EC_number="2.7.1.51"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417283.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008737 - L-fuculokinase activity
                     [Evidence IEA];
                     GO_process: GO:0042355 - L-fucose catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-fuculokinase"
                     /protein_id="HGU4548483.1"
                     /translation="MKQEVILVLDCGATNVRAIAVNRQGKIVARASTPNASDIAMENN
                     TWHQWSLDAILQRFADCCRQINSELTECHIRGIAVTTFGVDGALVDKQGNLLYPIISW
                     KCPRTAAVMDNIEQLISAQRLQAISGVGAFSFNTLYKLVWLKENHPQLLERAHAWLFI
                     SSLINHRLTGEFTTDITMAGTSQMLDIQQRDFSPQILQATGIPRRLFPRLVEAGEQIG
                     TLQNSAAAMLGLPVGIPVISAGHDTQFALFGAGADQNEPVLSSGTWEILMVRSAQVDT
                     SLLSQYAGSTCELDSQAGLYNPGMQWLASGVLEWVRKLFWTAETPWQMLIEEARLIAP
                     GADGVKMQCDLLSCQNAGWQGVTLNTTRGHFYRAALEGLTAQLQRNLQMLEKIGHFKA
                     SELLLVGGGSRNTLWNQIKANMLDIPVKVLDDAETTVAGAALFGWYGVGEFNSPEEAR
                     AQIHYQYRYFYPQTEPEFIEEV"
     gene            complement(11557..13332)
                     /gene="fucI"
                     /locus_tag="ACHOSC_RS12805"
                     /old_locus_tag="pgaptmp_002561"
     CDS             complement(11557..13332)
                     /gene="fucI"
                     /locus_tag="ACHOSC_RS12805"
                     /old_locus_tag="pgaptmp_002561"
                     /EC_number="5.3.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008736 - L-fucose isomerase activity
                     [Evidence IEA];
                     GO_function: GO:0030145 - manganese ion binding [Evidence
                     IEA];
                     GO_process: GO:0019317 - fucose catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-fucose isomerase"
                     /protein_id="HGU4548484.1"
                     /translation="MKKISLPKIGIRPVIDGRRMGVRESLEEQTMNMAKATAALLTEK
                     LRHACGAAVECVISDTCIAGMAEAAACEEKFSSQNVGLTITVTPCWCYGSETIDMDPT
                     RPKAIWGFNGTERPGAVYLAAALAAHSQKGIPAFSIYGHDVQDADDTSIPADVEEKLL
                     RFARAGLAVASMKGKSYLSLGGVSMGIAGSIVDHNFFESWLGMKVQAVDMTELRRRID
                     QKIYDEAELEMALAWADKNFRYGEDENNKQYQRNAEQRRAVLRESLLMAMCIRDMMQG
                     NSKLADIGRVEESLGYNAIAAGFQGQRHWTDQYPNGDTAEAILNSSFDWNGVREPFVV
                     ATENDSLNGVAMLMGHQLTGTAQVFADVRTYWSPEAIERVTGHKLDGLAEHGIIHLIN
                     SGSAALDGSCKQRDSEGNPTMKPHWEISQQEADACLAATEWCPAIHEYFRGGGYSSRF
                     LTEGGVPFTMTRVNIIKGLGPVLQIAEGWSVELPKDVHDILNKRTNSTWPTTWFAPRL
                     TGKGPFTDVYSVMANWGANHGVLTIGHVGADFITLASMLRIPVCMHNVEETKVYRPSA
                     WAAHGMDIEGQDYRACQNYGPLYKR"
     gene            complement(13365..14681)
                     /gene="fucP"
                     /locus_tag="ACHOSC_RS12810"
                     /old_locus_tag="pgaptmp_002562"
     CDS             complement(13365..14681)
                     /gene="fucP"
                     /locus_tag="ACHOSC_RS12810"
                     /old_locus_tag="pgaptmp_002562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015150 - fucose transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015756 - fucose transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-fucose:H+ symporter permease"
                     /protein_id="HGU4548485.1"
                     /translation="MGNTSIQTQSYRAVDKDAGQSRSYIIPFALLCSLFFLWAVANNL
                     NDILLPQFQLAFTLTNFQAGLIQSAFYFGYFIIPIPAGILMKKLSYKAGIITGLFLYA
                     LGAALFWPAAEIMNYTLFLVGLFIIAAGLGCLETAANPFVTVLGPESSGHFRLNLAQT
                     FNSFGAIIAVVFGQSLILSNVPHQSQDVLDKMSPEQLSAYKHSLVLSVQTPYMIIVAI
                     VLLVALLIMLTKFPALQSDNHSDAKQGSFSASLSRLARIRHWRWAVLAQFCYVGAQTA
                     CWSYLIRYAVEEIPGMTAGFAANYLTGTMVCFFIGRFTGTWLISRFAPHKVLAAYALI
                     AMALCLISAFAGGHVGLIALTLCSAFMSIQYPTIFSLGIKNLGQDTKYGSSFIVMTII
                     GGGIVTPVMGFVSDAAGNIPTAELIPALCFAVIFIFARFRSQTATN"
BASE COUNT         3641 a         3618 c         3888 g         3747 t
ORIGIN      
        1 cccctttcgg aagtcgcgaa tttaattaaa atattacaat cttgccaggg aaaagttgtt
       61 tttattggtg ttgggaaatc tggcattatt gccagaaaac ttgctgcaac atttgccagc
      121 accggaactc cctcgttttt tgttcacggt acggaagcgg tacacggcga ccttgggatg
      181 gtggcgaaag acgatgttgt tattcttatt tcaaacagtg gtgagacggc ggaaatcctg
      241 gcgacactgc ctagtttgaa aaaaatgggt aattatttga tctcttttac ccgcagtcat
      301 cactcatcgc ttgcgataag ttgtgattta tctgttgaaa tccccgtcaa aagtgaggcg
      361 gataatttag gtttagcacc gtcatgctcg tctactgttg ttctggttgt tggtgatgcg
      421 gtggcgcttg cactctctga attgaaaaaa tttactcgtg ctgatttcgg cttatatcat
      481 ccaggaggtg cactcggcat taaagcaaat tcataatttc aaagtataaa tacagtaacc
      541 ggtcacgcca tcttttgggg tggcgttcat tgaagtgagt gtcagctatg aaaaatgttc
      601 tggtcaccgt ccccagcttt tcagcacgct gtgtttccgc cagcaagttg ctaagagaga
      661 ataattttaa tcttattatt aagaataatg tggagcatct gcttaaatca gaatccactg
      721 cattacgaga atctatttgc gcggtcatcg ccggaaaaga ttgttatcag gcggacacgc
      781 tatcattgct gcctggtgtg aaaataatat cgagattcgg gacggggata gataatattg
      841 atcttcgtgc agcacaacaa tccggtattg tagtcaataa tgcggttggg attaattcaa
      901 atgctgtcgc ggaatttatc attggactta tcttcgcgag catgagaaat attccaggca
      961 gctatcatgc gatgcaaaat ggctactggg gtgagtcgca tggctatgaa ttacaaggca
     1021 aacggattgg catggttggc tacggcaata taggtaaaac cctggcaaaa aggttatccg
     1081 gttttgatgt tgagctttta gcttatgaca aacaacccga ttatcaggtt gctgacaaag
     1141 ttggagttca gtttgtatca attgaagata tctttatgca gtcgcatgtc atcattgttc
     1201 ttttgccttt ttctcctgag ctggagaact tcattagtca taaatatttg tcgatgatgc
     1261 gcaatggtgc tctaatcatc aatgccgcaa ggggaaagtt gctggatgag ggcgctttac
     1321 tacaggtgat tgaagaacga aatgtgtttg cggcgctgga tgtgtttagc tctgaaccgt
     1381 tggcgcaatt tagcccactg ctacatgcca aaaatatcat tacaacgccg catattgcgg
     1441 ccgccaccgt tgagtcatac cagcaaaccg gtacacacgt tgcccagtcg ataattgatt
     1501 acttcgcagg aagggagata aaaaacgtgt tgtgagttca gcgaacgaag tttttaaata
     1561 caaaaaaaac cgcaatttgt ggaacacatt gcggtttatc agcattctaa gtattggttg
     1621 cgggggccgg atttgaaccg acgaccttcg ggttatgagc ccgacgagct accaggctgc
     1681 tccaccccgc gtccgtggat gcgcactata ctccgctcgc gttttcatgc aacctttttt
     1741 ttaccccgat cacggattta gcatgatttt gaacaaaaac aaaccactaa agcctatttt
     1801 ttgtgcaaaa tttgcgtcag ggagaggctg ctcatttcat taaacgcggc ggtttgttat
     1861 cttcgttgcg ccttattttt taacctgaag aagagaacaa tgaaaggacg ttgggtaaag
     1921 taccttctta tgggcacggt tgtggcaatg cttgccgcct gctcttccaa accaaccgat
     1981 cgcggacagc aatataaaga cgggaaattt acccagcctt tctctctggt gaaccagcca
     2041 gatgccgttg gcgcgccgat taacgccggt gattttgccg agcaaattaa ccatatccgt
     2101 aattcgtcac cgcgtctgta tggcaaccag agtaatgttt ataacgcggt gcaagagtgg
     2161 ctgcgcgcag gcggtgatac ccgcaatatg cgccagttcg gcattgatgc ctggcagatg
     2221 gaaggtgccg acaactatgg taacgtgcag tttacaggct attacacgcc ggtaattcag
     2281 gcgcgccata cccgccaggg cgagttccag tatcctattt accgtatgcc gccaaaacgt
     2341 ggtcgtctgc cgtctcgtgc ggagatctac gcaggggcgc tgagtgataa atatattctc
     2401 gcttacagca actccctgat ggataacttc attatggatg tgcagggtag cgggtatatc
     2461 gactttggtg atggcagtcc gcttaacttt ttcagctatg cagggaaaaa cggtcatgcc
     2521 tatcgcagca ttggtaaggt gctgatcgac cgtggcgaag tgaaaaaaga agatatgtcg
     2581 atgcaggcga ttcgtcattg gggcgaaaca cacagtgaag ccgaggttcg cgagctgctg
     2641 gaacagaacc cgtctttcgt tttctttaaa ccgcaatctt ttgcaccggt gaaaggggca
     2701 agtgcggtgc cgctggttgg ccgtgcttct gttgcctccg atcgttcaat tattccgcca
     2761 ggtactacct tgctggcaga agtgccgctg ctggataata acggcaaatt taacggtcag
     2821 tacgaattgc gtctgatggt ggcgctggat gtcggtggcg caatcaaagg ccaacacttc
     2881 gatatctatc aagggatcgg gccggaagcc ggacatcgcg caggttggta caaccactat
     2941 ggacgtgtct gggtgctgaa aaccgccccg ggcgcaggta acgtctttag cggctgatgt
     3001 gttattctgt ataccgtttt tacggatacg acagggtgag ggataacctc acccttttta
     3061 attctgaggt gctatgtctg tggtaattag tgatgcatgg cgtcagcgtt ttggtggcac
     3121 agcgcgtctg tatggtgaaa aagcgttgca actgtttgct gacgcgcata tttgtgtggt
     3181 tggtatcggt ggtgtcggtt cctgggcggc ggaagcgctg gcgcgcacgg ggattggcgc
     3241 aatcacgctt atcgatatgg atgatgtgtg cgtcaccaat accaatcggc aaattcacgc
     3301 tctgcgcgat aacgtcgggc tggcaaaagc ggaagtcatg gcggagcgta ttcgccagat
     3361 taacccggag tgccgtgtaa cggtggtgga tgatttcgtg acgccggata acgtagcgca
     3421 gtatatgagc gtggggtatt cgtacgtgat tgatgccatt gatagtgtac ggcccaaagc
     3481 ggcgctgatt gcttattgtc ggcgcaataa aatcccgctg gtcacgaccg gtggcgcggg
     3541 tgggcagatt gacccgacgc agattcaggt taccgatctg gcgaaaacga ttcaggatcc
     3601 gctggcggcg aagttgcgcg agcgcctgaa aagcgatttt ggcgtagtga aaaacagtaa
     3661 aggtaagctc ggcgtcgatt gcgtgttttc tactgaagcg ctggtgtacc cgcagtcaga
     3721 cggtactgtg tgtgcgatga aagccacggc agaagggccg aagcggatgg attgtgcatc
     3781 tggatttggc gcggcaacga tggtgaccgc cacctttggg tttgttgcgg tttctcatgc
     3841 gctgaagaag atgatggcga aagcggcgcg tcagggttaa acaatcaggt cggatgcgac
     3901 gcgaacgtct tatccgacct ggttctgtgc ttaaacctgc ttcgtcgcag cgataatcgc
     3961 ctcgcttaac gcattcaacc cctggctgcg tgaggcgcta agctgcccac gcaatcccag
     4021 ctcatcaaac aatgccagcg gcgactgtgc ctgcaattcg gcggcggttt tcccctcaac
     4081 ggcagtcaac agcaccgcca gcaggccgcg cactatacgt ccttcgctgt cgccaaagaa
     4141 gtgcatttta ccgttttcag ccacggtata tcccagccag acgcggtttt cgcatccggc
     4201 aatctcttta gcctgcgctt ttaactcgtc tggcaaagcc ggaagctgtt tccccagcat
     4261 gatcaactgg cgatatttat cttcccattg cgtcaacggt gcgaaggtat tgcgtaacgt
     4321 ttctgcggtt acggttgtgc cgaacggatg tccggcgaat tgcgggttcg tcattaatcc
     4381 accaataatt ccagcgcgcg gtcaacggca ttcaccagcg catccacatc actctttgta
     4441 ttatatggcg caaaagaggc gcgcagtgtg ccggttacgc ctaattctgc cagtagcggc
     4501 tgagcgcaat gttgcccggc ccgcaaggca ataccgtact ccgccagcag cgtcaccata
     4561 tcgctgtgat gaacgccagc aaaatcaaag gccagcaggc tggaatcctg gcagcggaat
     4621 gaacggaaac ctggacgttt cgccagcgca tcttccgcca gtgttgctaa gctacggctc
     4681 cagttttcgg cctggttgat atcgtaatct gccagccatt ccagcgccgc gcttaatcct
     4741 atgacaccag ccacatttgg cgttcctgct tccagtttcc acggcgcaga ttgagtcgtg
     4801 aagccgtcaa aactcacttc gtgaaccatt ttgccgccgc ccagccaggg cgacatcgct
     4861 tccagcagtt ctgatttgcc atacagcacg ccgatacctg tcgggccata cagtttgtga
     4921 cctgaaaaag catagaaatc aatattcagt tgctgaacat ccgcggggaa atgcaccgcc
     4981 ccctgagcac catcaaccat caccaccatc ccggctgaat gagcaaaggt aatcgctcgc
     5041 gccagatccg ggcaaccgcc agtaacgttc gacatctgac ccaacgccag aatccgacta
     5101 cggggagtaa tcagttctgg caacaaatcg acatccggca gtcgctgcgc attaagcggc
     5161 aatttcacca ctttggctcc agtttgttgg gcgaccatca gccaggggac gaggttggcg
     5221 tggtgttctg ccacgctgac aataatctca tcgcccagtt gcagacgcgg acgcgcatag
     5281 cattgtgcaa ccatgttgat ggattcagtg gtgccgcgcg tccagacgat agttttatca
     5341 tccggtgcat tcagtaattg cgccactttc tctcgtgcag cttcataacg cgcggtcagg
     5401 cgttgggctt cggcaaactg gctgcgatgg acgtttccgg cgctcaggct gtaaaactgt
     5461 cgggtggctt caaccacggc ttcaggttta agcgcggtcg cggcgctgtc gagatagacg
     5521 cccgcatcct gtagagcggg aaactgggcg cgaaactgcg cgggattaaa aacgttcatg
     5581 gtactcctcg gcttgatgca ccgatcgtga cgcaaaattc actttgatac aaggagagtg
     5641 ctaaccatca gaattatctg gatatcctgg cgaagccgcg taagtaacat atgctgaata
     5701 gtgttaggcg ttaattttag cctgttaatc aatactatta agatttatta atcgctttaa
     5761 ggagaagatg atgaaaaaga ctgccgcaat tatttctgcc tgtatgctga cttttgccct
     5821 gagcgcctgt tccggttcga actatgtgat gcacaccaat gacggacgta ccatcgtctc
     5881 tgacggcaaa ccacagactg ataacgatac cggtatgatt tcgtataaag acgctaatgg
     5941 caacaaacag cagatcaacc gtactgacgt gaaagagatg gtcgaactgg atcagtaatt
     6001 cacgatcgca aggtaaaaaa aagcaccgca attaggcggt gctacattaa tcactatgga
     6061 cagacagggt aaatgtacag gaagtgaaaa aaggtagctt tgctaccatg gtctgaatcg
     6121 cagaccaatt gcaaacacaa caacacaaca tcacaaccgt aagccaaaag ttcaccagaa
     6181 cacgcattcc gataaaactt ttcgttccgg ctcaggaagt gccgccacta taggtatttg
     6241 ctggtagaag ctcaacggac aatttataat ggctcagatt aaaaaaacta ataggttaca
     6301 cagtgtgatc taattgttaa attcatttaa catcaaagtt taaaagccat gtctaaacga
     6361 ttaccaccgc taaatgcctt acgagttttt gatgccgcag cacgccattt aagtttcact
     6421 cgcgcagcag aagagctttt tgtgacccag gccgcagtaa gtcatcaaat caagtctctt
     6481 gaggattttt tggggctaaa actgttccgc cgccgtaatc gttcactcct gctgaccgag
     6541 gaagggcaaa gctatttcct cgatatcaaa gagatatttt cgcaattaac cgaagcgacg
     6601 cgtaaactcc aggcccgtag cgccaagggg gcgttgacgg tcagtttact ccccagtttc
     6661 gccattcatt ggttggttcc gcgactttcc agctttaatt cagcttatcc gggaattgac
     6721 gttcgaatcc aggcggttga tcgtcaggaa gataagctgg cggatgatgt tgatgtggcg
     6781 atattttatg gtcggggcaa ctggccgggg ctacgggtgg aaaaactgta cgccgaatat
     6841 ttattgccgg tgtgttcgcc gctactgctg acaggcgaaa aacccttgaa gaccccggaa
     6901 gatctggcta aacatacgtt attacatgat gcgtcacgcc gtgactggca gacatatacc
     6961 cgacagttgg ggttaaatca tatcaacgtt cagcaagggc caatttttag tcatagcgcc
     7021 atggtgctgc aagcggctat tcacgggcag ggagtggcgc tggcaaataa cgtgatggcg
     7081 caatctgaaa ttgaagccgg acgtcttgtt tgcccgttta atgatgttct ggtcagtaaa
     7141 aacgcttttt atctggtttg tcatgacagc caggcagaac tgggtaaaat agccgccttt
     7201 cgccaatgga tcctggcgaa agccgctgct gaacaagaaa aattccgctt tcgttatgaa
     7261 caataattta cgtagggtac gaccatgacc agccgtttta tgctgatttt cgccgccatt
     7321 agcggcttca tttttgtggc tctgggcgct tttggcgcgc atgtgttaag taaaaccatg
     7381 ggggccgttg agatgggctg gatccagacc ggcctcgaat accaggcgtt tcatacgctg
     7441 gcgatcttag gtctggcggt ggcaatgcag cgtcgcatca gtatctggtt ttactggagt
     7501 agcgttttcc tcgcgttagg cacggtgctg ttcagcggca gcctttattg cctggcactg
     7561 tcccatctgc gtttgtgggc gtttgtcact ccggttggcg gcgtgagctt ccttgcgggc
     7621 tgggcgttaa tgttagttgg tgctatccgt ttaaagcgca agggcgtaag tcatgaataa
     7681 ggttgtattg ctgtgccgtc cgggctttga aaaagagtgc gccgcagaaa ttaccgataa
     7741 agccggccag cgggaaattt tcggttttgc ccgcgtgaaa gagaatgcgg gttatgtcat
     7801 ttatgaatgt tatcaacctg atgatggcga taagttaatc cgtgagctgc cgttcagttc
     7861 attaattttt gcccgccagt ggtttgtggt gggggaactc ctgcagcatt tgccgccaga
     7921 agatcgtatt acccccattg tcggcatgtt acagggcgta gtagagaagg gcggtgaact
     7981 gcgtgttgaa gttgccgata ccaacgaaag caaagagttg ctgaaattct gccgtaaatt
     8041 caccgtgccg ctacgcgctg ccttgcgcga tgccggggtg ctggcgaact atgaaacgcc
     8101 gaagcgtccg gttgtgcatg tattcttcat tgcaccaggt tgctgctata ccggttactc
     8161 atacagcaac aataattcgc cgttctatat gggcattccg cgcctgaaat ttccggcaga
     8221 tgcgccgagt cgttccacac tcaaactgga agaggcattt catgtgttta ttcctgcgga
     8281 tgagtgggat gaacgcctgg cgaacgggat gtgggcggtg gatttgggcg cttgcccagg
     8341 cggctggacc taccaactgg tgaagcgcaa catgtgggtt tattccgtcg acaacggccc
     8401 gatggcgcaa agtctgatgg ataccgggca ggtgacgtgg ctgcgggaag acggtttcaa
     8461 attccgtccg acacgcagca atatctcctg gatggtatgc gatatggttg aaaaaccggc
     8521 taaagttgcg gcattgatgg cgcagtggct ggttaatggc tggtgccgtg aaaccatttt
     8581 caatctcaaa ctgccgatga aaaaacgcta cgaagaagta tcacacaatc tggcgtatat
     8641 tcaggcacag cttgatgaac atggcataaa tgctcagatt caggcgcggc agttgtatca
     8701 cgatcgcgaa gaagtgacgg tgcacgtccg ccgcatctgg gctgcggtgg gtggtcgtcg
     8761 cgacgagcga taacaggcaa ggccggggcg tcgtctccgg cctgctaccc tttccgtcag
     8821 gctgttacca aagaagttgc aacctggcgc tcatcagaaa taatgtgcgt tacctcatcc
     8881 agatgcccga tgcgggcttc ccctgaacga ttaaatttac ttttatcaat caataacaac
     8941 gattgcgcgg cacgttttaa tagcatcgat ttgtaatcag cgttgatggc attggagtcc
     9001 cacagtgcgc cgctgctatc gatcccttca caagaaaaaa taaacagatc gatttccagc
     9061 gatttaagtt gggaaatcag cgagggattg acgtaacagc catatttgcg ctcaagtgtg
     9121 ccgccggaac tgatcagttg aatgcgttcg cgtttaccga gttcatggca aatcggatgg
     9181 ctattggtga agacctgaat gttgatgtca ggcaactggc gtgccagata ccagcaagtt
     9241 gaactggcgt ctaaggctat caccatccct tcttcaatcc acgcgagcgc ctcgcgcgcg
     9301 atatctgctt tatgcgcata atggcttttc agccggatgt gaaagggatc gccactgtct
     9361 tgattttgac ggtggatata tttagcgcgt ccatgattgc gcagaatttt accctgcgtc
     9421 tgtaattcat tgagatcgcg acgaatggtt tctttactga cctttagctg ttcagagaga
     9481 gcttccgtgg tcaggctggt atggttcagc agcaggtcga ctatcgcttg ctggcgtgcc
     9541 gctttcatat ttttcacccc atcattcgat aaccgcccgc gtcatgcggg cgtaccggta
     9601 tgagattacg gtgttacccc tttttttaaa agaatattcc cgtacttcgc tcgttcgcct
     9661 gtgataacga tcgcaaaggc tttttgcgcc cgttcataaa acgcaaaacg attgatgcgg
     9721 atgatgtcag gacacggggc ttgtagtgaa agcgcattac ggtaacgtcg ttctacttca
     9781 ggatcgagag tgtcaccttc taccgctgcc atcatcacca gcggcggggc ataactgtcc
     9841 agctcaaata acgggataat cgcctggagc aagtcgctca ccaacaggcc atcagcgcgg
     9901 atcacctgcg gtcccatcga atgggcggga aagtgagcat cggaaaaaat aatttcatct
     9961 ccatgtccca tctctgccag cactttcaat agttcgggag aaattaacgg cgaaattgtt
    10021 ttcagcattt cacacttcct ctataaattc aggttcagtt tgcgggtaga aataacggta
    10081 ctgataatga atctgtgcac gggcttcttc cgggctgttg aattccccta cgccatacca
    10141 accgaacagc gcagctcctg cgacggtcgt ttcggcatcg tcgagaactt ttaccggaat
    10201 atcaagcata ttggctttaa tctgattcca caatgtgttg cgacttcctc caccgactaa
    10261 caataattca gaggccttaa agtgcccgat tttttccagc atctgtagat tgcgctgtaa
    10321 ttgcgcagtt aacccttcca gcgccgcgcg atagaaatgc ccccgcgtgg tattaagcgt
    10381 cactccttgc cagccagcgt tctgacacga caataaatca cactgcattt ttacgccatc
    10441 cgcgccaggc gcgatcagac gagcttcttc aatcaacatt tgccagggtg tttcagccgt
    10501 ccagaacagt tttctcaccc attccagcac gccggatgcc agccattgca tacctgggtt
    10561 atacaaccct gcctggctat ccagttcgca ggtggaaccg gcgtactgac ttaacagcga
    10621 agtatcaacc tgggcgctac ggaccattaa aatttcccat gtaccggaag agagcacggg
    10681 ttcattttgg tcggcaccag cgccaaaaag ggcgaactgg gtatcgtgac ctgcggaaat
    10741 caccggtatg ccaacgggta agccgagcat tgctgcggcg ctgttctgta gcgtaccaat
    10801 ctgttcaccc gcttccacca gacgagggaa gagtcggcgt ggaataccgg tggcttgtaa
    10861 aatttgcgga ctgaaatcgc gttgctggat atccagcatc tggctggttc cggccatcgt
    10921 gatatcagta gtgaattcgc cggttaaacg gtggttaatc agcgacgaaa taaagagcca
    10981 ggcgtgcgcg cgttccagca gttgtggatg attttctttc aaccacacca acttatataa
    11041 cgtattgaaa ctaaaggctc cgacgccaga aatagcctgc aaccgctgtg cggagatcaa
    11101 ctgttcaata ttgtccataa ccgctgctgt tcgcggacat ttccagctaa tgatcggata
    11161 gagcagattg ccttgcttat ctaccagagc gccatccaca ccaaaggtgg tgacggcgat
    11221 accgcggatg tggcattcag tcagttcact attgatttgc cgacagcaat cagcaaagcg
    11281 ttgcaaaatg gcgtctaaag accactggtg ccaggtgttg ttttccatcg cgatatcgct
    11341 ggcattaggc gttgaggcgc gggcaacaat ttttccctgc cgattaaccg cgatggccct
    11401 gacattggtc gcgccacagt cgagtaccag gataacttct tgtttcataa tcgctcctgc
    11461 aatatagccg gataacattg cttatccggc taaccactct tgcttgttca ggcccggtag
    11521 ccggagcgac cgggcatcac atcagggagt aatgtattaa cgcttgtaca acgggccgta
    11581 gttctggcaa gcgcggtaat cctggccttc aatatccatg ccgtgcgcag cccaggcaga
    11641 aggacgatac actttggtct cttcaacgtt gtgcatacat accgggatac gcagcatgga
    11701 ggcgagagtg ataaagtctg cgccaacgtg gccgatggtc agaaccccat ggttagcgcc
    11761 ccagttcgcc attaccgagt acacatccgt aaacggccct ttaccggtga ggcgcggtgc
    11821 aaaccaggtg gttggccagg ttgagttggt gcgtttgttg aggatgtcat gcacatcctt
    11881 cggcaattcc acgctccagc cttccgcgat ttgcagtacc ggtcccaggc ctttgatgat
    11941 gttgacacgg gtcatggtga acgggacgcc gccttcggta aggaagcggg aagagtaacc
    12001 gccgccacgg aagtattcgt ggatcgccgg gcaccattcg gtagcggcga ggcaagcgtc
    12061 agcctcttgc tgagagattt cccagtgcgg cttcatcgtc gggttacctt cgctgtcgcg
    12121 ttgtttacag gaaccgtcca gcgcagcaga accggagttg atcaaatgga tgatgccgtg
    12181 ttctgccagt ccatcgagtt tatgccccgt tacacgctca attgcttctg gtgaccagta
    12241 ggtacgcaca tcggcaaata cctgagcggt gccggtgagc tggtgaccca ttagcattgc
    12301 cacgccgtta agactgtcgt tttcggtcgc cacgacaaag ggttcgcgca cgccattcca
    12361 gtcaaatgaa ctgttaagga tcgcttcggc ggtgtcacca ttgggatatt gatcggtcca
    12421 gtgacgttgc ccctggaagc ccgcagcgat ggcgttgtag ccaagtgatt cttccacgcg
    12481 accaatatcg gccagtttgc tgttgccttg catcatgtcg cggatacaca tcgccatcag
    12541 taaactttcg cgcagaactg cgcggcgttg ctcggcattg cgttgatact gtttgttatt
    12601 ttcatcttcg ccatagcgga agtttttatc agcccaggcc agtgccattt ccagttcggc
    12661 ttcgtcgtaa atcttctgat cgatacggcg acgcagttcg gtcatatcca ccgcctggac
    12721 tttcattccc agccaggatt caaagaagtt gtgatcaaca atggaaccgg cgatacccat
    12781 cgaaacgccg cccagcgaca gatagctttt acctttcatg ctggcgacgg ccaaaccggc
    12841 gcgggcaaag cgcagcagtt tttcttcaac atcggcagga atcgatgtgt catcggcatc
    12901 ctgaacgtca tgaccgtaaa tggagaatgc cgggatgcct ttctggctgt gagctgccag
    12961 agccgctgcc aggtaaacag cgccgggacg ttcagtgccg ttaaagcccc aaatggcttt
    13021 cgggcgggtt ggatccatgt cgatggtttc actgccatag caccagcaag gcgttaccgt
    13081 aatggtgagg cctacattct gactgctgaa tttttcttcg caagcagcgg cttcagccat
    13141 acccgcgata caggtatcgg aaatgacaca ctcgacggca gctccgcagg catggcgcag
    13201 tttctcggtc agcagtgcgg ccgtagcttt cgccatattc attgtttgtt cttcaagcga
    13261 ctcacgaaca cccatgcgac gaccgtcaat aaccgggcgg ataccaattt tcggtaagct
    13321 gatttttttc attacagatt cctcacttta ttcggaaaat atgttcagtt agttgccgtt
    13381 tgagagcgga aacgggcaaa gataaagatg accgcgaagc agagtgcggg gatcagttca
    13441 gcagtgggga tgttgcccgc cgcgtcactg acaaaaccca tgaccggagt gacaataccg
    13501 ccgccaatga tggtcataac gatgaaggac gaaccgtatt tggtgtcctg gccgagattc
    13561 ttaatgccca gcgagaagat tgttgggtac tgaatcgaca taaaggcgct gcataaagtc
    13621 agggctatta agcccacatg accgccagcg aaggctgaga tcaggcacag tgccatagcg
    13681 attaatgcgt aggcggccag gactttgtgt ggtgcgaagc gactgatgag ccaggtaccg
    13741 gtgaaacgac caataaagaa gcacaccatg gttccggtta aatagttagc ggcaaagcct
    13801 gcagtcatac ctggaatttc ttctacagcg tagcgaatca aatagctcca gcaggccgtt
    13861 tgtgcgccaa cgtagcagaa ttgcgctaat actgcccagc gccagtggcg aatacgcgcc
    13921 aggcgagaaa gcgatgcgga gaacgatcct tgtttggcgt cactgtgatt atcactctgc
    13981 aatgccggga atttcgtcag catgatcagc agggcgacca gtaacacgat agccacgatg
    14041 atcatataag gtgtctgtac cgataatacc aggctgtgtt tatacgcact caattgctct
    14101 ggagacattt tatcgagaac gtcttgcgat tgatgtggca cgttagacaa aataagactt
    14161 tgcccaaaga caaccgcgat aattgcgcca aacgagttaa atgtttgcgc aagatttaag
    14221 cggaagtgac cactgctttc cggccctaat accgtaacaa aagggtttgc ggcagtttcc
    14281 agacaaccta atccggctgc aataataaat aggccaacta aaaataaggt gtagttcatt
    14341 atttctgcag cgggccagaa taatgcagcg cccaaggcat ataaaaataa tccggtaata
    14401 atccctgctt tataactgag ttttttcatc aatatcccag cagggattgg gataatgaaa
    14461 taaccaaagt aaaaggccga ttggatcagg ccagcctgga aatttgtcag cgtaaaagcc
    14521 agctggaatt gaggtaataa aatgtcgtta aggttattgg ctaccgccca aagaaaaaac
    14581 agtgagcaca gcagcgcgaa tggaataatg taacttctgc tttgccctgc atctttatct
    14641 accgcacggt aactctgcgt ttgtattgat gtgtttccca tagcatcctc ttaggttcag
    14701 aagcttaagc acgtaatcgg ttatgttttc aaaagtgcaa aaccagcgag ttcgggcttc
    14761 atatcagtga atatttgcct gtctgaaatg aactcatgga ttcattttca aataaaaaac
    14821 gctctgtaat atgacggcgg tcacacttat tagctggaag gtgactttat gtgactacca
    14881 tcactttaat aatc
//
LOCUS       DAZKHF020000103        14838 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2201S31241
            SAMN43267800-rid25106413.denovo.104, whole genome shotgun sequence.
ACCESSION   DAZKHF020000103 DAZKHF020000000
VERSION     DAZKHF020000103.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267800
            Sequence Read Archive: SRR30307263
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 14838)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14838)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 14838)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350581.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350581.2
            Genome Coverage       :: 151x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:21:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,652
            CDSs (total)                      :: 5,557
            Genes (coding)                    :: 5,320
            CDSs (with protein)               :: 5,320
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 237
            CDSs (without protein)            :: 237
            Pseudo Genes (ambiguous residues) :: 0 of 237
            Pseudo Genes (frameshifted)       :: 102 of 237
            Pseudo Genes (incomplete)         :: 130 of 237
            Pseudo Genes (internal stop)      :: 52 of 237
            Pseudo Genes (multiple problems)  :: 41 of 237
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14838
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267800-rid25106413.denovo.104"
                     /serotype="O 26:H11"
                     /isolate="2201S31241"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-01"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            67..1356
                     /gene="hpaG"
                     /locus_tag="ACHOSC_RS12815"
                     /old_locus_tag="pgaptmp_002563"
     CDS             67..1356
                     /gene="hpaG"
                     /locus_tag="ACHOSC_RS12815"
                     /old_locus_tag="pgaptmp_002563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710093.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008704 -
                     5-carboxymethyl-2-hydroxymuconate delta-isomerase activity
                     [Evidence IEA];
                     GO_function: GO:0018800 -
                     5-oxopent-3-ene-1,2,5-tricarboxylate decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:1901023 - 4-hydroxyphenylacetate catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxyphenylacetate degradation bifunctional
                     isomerase/decarboxylase"
                     /protein_id="HGU4548486.1"
                     /translation="MKGTIFVVALNHRSQLDAWQEAFQQSPYKAPPKTAVWFIKPRNT
                     VIGCGEPIPFPQGEKVLSGATVALIVGKTATKVREEDAAEYIAGYALANDVSLPEESF
                     YRPAIKAKCRDGFCPIGETVALSNVDNLTIYTEINGRPADHWNTADLQRNAAQLLSAL
                     SEFATLNPGDAILLGTPQARVEIQPGDRVRVLAEGFPPLENPVVDEREVTTRKSFPTQ
                     PHPHGTLFALGLNYADHASELEFKPPEEPLVFLKAPNTLTGDNQTSVRPNNIEYMHYE
                     AELVVVIGKQARNVSEADAMDYVAGYTVCNDYAIRDYLENYYRPNLRVKSRDGLTPML
                     STIVPKEAIPDPHNLTLRTFVNGELRQQGTTADLIFSVPFLIAYLSEFMTLNPGDMIA
                     TGTPKGLSDVVPGDEVVVEVEGVGCLVNRIVSEETAK"
     gene            1353..2819
                     /gene="hpaE"
                     /locus_tag="ACHOSC_RS12820"
                     /old_locus_tag="pgaptmp_002564"
     CDS             1353..2819
                     /gene="hpaE"
                     /locus_tag="ACHOSC_RS12820"
                     /old_locus_tag="pgaptmp_002564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018480 -
                     5-carboxymethyl-2-hydroxymuconic-semialdehyde
                     dehydrogenase activity [Evidence IEA];
                     GO_process: GO:1901023 - 4-hydroxyphenylacetate catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-carboxymethyl-2-hydroxymuconate semialdehyde
                     dehydrogenase"
                     /protein_id="HGU4548487.1"
                     /translation="MKKVNHWINGKNVAGNDYFQTTNPATGEVLADVASGGEAEINQA
                     VAAAKEAFPKWANLPMKERARLMRRLGDLIDQNVPEIAAMETADTGLPIHQTKNVLIP
                     RASHNFEFFAEVCQQMNGKTYPVDDKMLNYTLVQPVGVCALVSPWNVPFMTATWKVAP
                     CLALGNTAVLKMSELSPLTADRLGELALEAGIPAGVLNVVQGYGATAGDALVRHHDVR
                     AVSFTGGTATGRNIMKNAGLKKYSMELGGKSPVLIFEDADIERALDAALFTIFSINGE
                     RCTAGSRIFIQQSIYPEFVKRFAERANRLRVGDPNDPNTQVGALISQQHWEKVSGYIR
                     LGIEEGATLLAGGPDKPSDLPAHLKGGNFLRPTVLADVDNRMRVAQEEIFGPVACLLP
                     FKDEAEGLRLANDVEYGLASYIWTQDVSKVLRLARGIEAGMVFVNTQNVRDLRQPFGG
                     VKASGTGREGGEYSFEVFAEMKNVCISMGDHPIPKWGV"
     gene            2821..3672
                     /gene="hpaD"
                     /locus_tag="ACHOSC_RS12825"
                     /old_locus_tag="pgaptmp_002565"
     CDS             2821..3672
                     /gene="hpaD"
                     /locus_tag="ACHOSC_RS12825"
                     /old_locus_tag="pgaptmp_002565"
                     /EC_number="1.13.11.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008198 - ferrous iron binding [Evidence
                     IEA];
                     GO_function: GO:0008687 - 3,4-dihydroxyphenylacetate
                     2,3-dioxygenase activity [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3,4-dihydroxyphenylacetate 2,3-dioxygenase"
                     /protein_id="HGU4548488.1"
                     /translation="MGKLALAAKITHVPSMYLSELPGKNHGCRQGAIDGHKEISKRCR
                     EMGVDTIIVFDTHWLVNSAYHINCADHFEGVYTSNELPHFIRDMTYNYEGNPELGQLI
                     ADEALKLGVRAKAHNIPSLKLEYGTLVPMRYMNEDKHFKVVSISAFCTVHDFADSRKL
                     GEAILKAIEQYDGTVAVLASGSLSHRFIDDQRAEEGMNSYTREFDRQMDERVVKLWRE
                     GQFKEFCNMLPEYADYCYGEGNMHDTVMLLGMLGWDKYDGKVEFITELFPSSGTGQVN
                     AVFPLPA"
     gene            3682..4062
                     /locus_tag="ACHOSC_RS12830"
                     /old_locus_tag="pgaptmp_002566"
     CDS             3682..4062
                     /locus_tag="ACHOSC_RS12830"
                     /old_locus_tag="pgaptmp_002566"
                     /EC_number="5.3.3.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-carboxymethyl-2-hydroxymuconate
                     Delta-isomerase"
                     /protein_id="HGU4548489.1"
                     /translation="MPHFIVECSDNIREEADLPGLFAKVNPTLAATGIFPLAGIRSRV
                     HWVDTWQMADGQHDYAFVHMTLKIGAGRSLESRQQAGEMLFELIKTHFAALMESRLLA
                     LSFEIEELHPTLNFKQNNVHALFK"
     gene            4173..4976
                     /gene="hpaH"
                     /locus_tag="ACHOSC_RS12835"
                     /old_locus_tag="pgaptmp_002567"
     CDS             4173..4976
                     /gene="hpaH"
                     /locus_tag="ACHOSC_RS12835"
                     /old_locus_tag="pgaptmp_002567"
                     /EC_number="4.2.1.163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710089.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018817 - 2-oxo-hept-3-ene-1,7-dioate
                     hydratase activity [Evidence IEA];
                     GO_process: GO:0009056 - catabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxo-hept-4-ene-1,7-dioate hydratase"
                     /protein_id="HGU4548490.1"
                     /translation="MFDKHTHTLIAQRLDQAEKQREQIRAISLDYPEITIEDAYAVQR
                     EWVRLKIAEGRTLKGHKIGLTSKAMQASSQISEPDYGALLDDMFFHDGSDIPTDRFIV
                     PRIEVELAFVLAKPLRGPNCTLFDVYNATDYVIPALELIDARCHNIDPETQRPRKVFD
                     TISDNAANAGVILGGRPIKPDELDLRWISALMYRNGVIEETGVAAGVLNHPANGVAWL
                     ANKLAPYDVQLEAGQIILGGSFTRPVPARKGDTFHVDYGNMGSISCRFV"
     gene            4987..5775
                     /gene="hpaI"
                     /locus_tag="ACHOSC_RS12840"
                     /old_locus_tag="pgaptmp_002568"
     CDS             4987..5775
                     /gene="hpaI"
                     /locus_tag="ACHOSC_RS12840"
                     /old_locus_tag="pgaptmp_002568"
                     /EC_number="4.1.2.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015966060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018802 -
                     2,4-dihydroxyhept-2-ene-1,7-dioate aldolase activity
                     [Evidence IEA];
                     GO_process: GO:0010124 - phenylacetate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-2-oxoheptanedioate aldolase"
                     /protein_id="HGU4548491.1"
                     /translation="MENSFKAALKAGRPQIGLWLGLSSSYSAELLAGAGFDWLLIDGE
                     HAPNNVQTVLTQLQAIAPYPSQPVVRPSWNDPVQIKQLLDVGTQTLLVPMVQNADEAR
                     EAVRATRYPPAGIRGVGSALARASRWNRIPDYLQKANDQMCVLVQIETREAMKNLPQI
                     LDVEGVDGVFIGPADLSADMGYAGNPQHPEVQAAIEQAIVQIREAGKAPGILIANEQL
                     AKRYLELGALFVAVGVDTTLLARAAEALAARFGAQATAIKPDVY"
     gene            5950..7326
                     /gene="hpaX"
                     /locus_tag="ACHOSC_RS12845"
                     /old_locus_tag="pgaptmp_002569"
     CDS             5950..7326
                     /gene="hpaX"
                     /locus_tag="ACHOSC_RS12845"
                     /old_locus_tag="pgaptmp_002569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_460080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:1901241 - 4-hydroxyphenylacetate
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:1900754 - 4-hydroxyphenylacetate transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxyphenylacetate permease"
                     /protein_id="HGU4548492.1"
                     /translation="MSDTSPAIPESIDPANQHKALTAGQQAVIKKLFRRLIVFLFVLF
                     IFSFLDRINIGFAGLTMGRDLGLSATMFGLATTLFYAAYVIFGIPSNIMLSIVGARRW
                     IATIMVLWGIASTATMFATGPTSLYVLRILVGITEAGFLPGILLYLTFWFPAYFRARA
                     NALFMVAMPVTTALGSIVSGYILSLDGVMALKGWQWLFLLEGFPSVLLGVMVWFWLDD
                     SPDKAKWLTKEDKKCLQEMMDNDRLTLVQPEGAISHHAMQQRSMWREIFTPVVMMYTL
                     AYFCLTNTLSAISIWTPQILQSFNQGSSNITIGLLAAVPQICTILGMIYWSRHSDRRQ
                     ERRHHTALPYLFAAAGWLLASATDHNMIQMLGIIMASTGSFSAMAIFWTTPDQSISLR
                     ARAIGIAVINATGNIGSALSPFMIGWLKDLTGSFNSGLWFVATLLVIGAGIIWAIPMQ
                     SSRPRATP"
     gene            7336..8226
                     /gene="hpaA"
                     /locus_tag="ACHOSC_RS12850"
                     /old_locus_tag="pgaptmp_002570"
     CDS             7336..8226
                     /gene="hpaA"
                     /locus_tag="ACHOSC_RS12850"
                     /old_locus_tag="pgaptmp_002570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020801836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxyphenylacetate catabolism regulatory
                     protein HpaA"
                     /protein_id="HGU4548493.1"
                     /translation="MCDRQIANIDISKEYDESLGTDDVHYQSFARMAAFFGRHMLPHR
                     HEQYFQMHFLNSGQIELQLDDHRYSVEAPLFVLTPPSVPHAFITESDADGHVLTVRED
                     LIWPLLEVLYPGTRETFGLPGICLSLADKPDELAALEHYWQLIERESVEQLPGREHTL
                     TLLAQAVFTLLLRNAKLDDHAASGMRGELKLFQRFHMLIESHFHQHWTVPDYANELHI
                     TESRLTDICRRFANRPPKRLIFDRQLREAKRLLLFSDNAVNNIAWQLGFKDPAYFARF
                     FNRLVGCSPSAYRAKKVPVT"
     gene            8477..10039
                     /gene="hpaB"
                     /locus_tag="ACHOSC_RS12855"
                     /old_locus_tag="pgaptmp_002571"
     CDS             8477..10039
                     /gene="hpaB"
                     /locus_tag="ACHOSC_RS12855"
                     /old_locus_tag="pgaptmp_002571"
                     /EC_number="1.14.14.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000801462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016712 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen, reduced flavin or flavoprotein as one
                     donor, and incorporation of one atom of oxygen [Evidence
                     IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0010124 - phenylacetate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxyphenylacetate 3-monooxygenase,
                     oxygenase component"
                     /protein_id="HGU4548494.1"
                     /translation="MKPEDFRASTQRPFTGEEYLKSLQDGREIYIYGERVKDVTTHPA
                     FRNAAASVAQLYDALHKPEMQDSLCWNTDTGSGGYTHKFFRVAKSADDLRQQRDAIAE
                     WSRLSYGWMGRTPDYKAAFGCALGANPGFYGQFEQNARNWYTRIQETGLYFNHAIVNP
                     PIDRHLPTDKVKDVYIKLEKETDAGIIVSGAKVVATNSALTHYNMIGFGSAQVMGENP
                     DFALMFVAPMDADGVKLISRASYEMVAGATGSPYDYPLSSRFDENDAILVMDNVLIPW
                     ENVLIYRDFDRCRRWTMEGGFARMYPLQACVRLAVKLDFITALLKKSLECTGTLEFRG
                     VQADLGEVVAWRNTFWALSDSMCSEATPWVNGAYLPDHAALQTYRVLAPMAYAKIKNI
                     IERNVTSGLIYLPSSARDLNNPQIDQYLAKYVRGSNGMDHVQRIKILKLMWDAIGSEF
                     GGRHELYEINYSGSQDEIRLQCLRQAQSSGNMDKMMAMVDRCLSEYDQNGWTVPHLHN
                     NDDINMLDKLLK"
     gene            10057..10569
                     /locus_tag="ACHOSC_RS12860"
                     /old_locus_tag="pgaptmp_002572"
     CDS             10057..10569
                     /locus_tag="ACHOSC_RS12860"
                     /old_locus_tag="pgaptmp_002572"
                     /EC_number="1.14.14.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxyphenylacetate 3-monooxygenase reductase
                     subunit"
                     /protein_id="HGU4548495.1"
                     /translation="MQLDEQRLRFRDAMASLSAAVNIITTEGDAGQCGITATAVCSIT
                     DTPPSLMVCINANSAMNPVFQGNGKLCVNVLNHEQELMARHFAGMTGMAMEERFSLSC
                     WQKGPLAQPVLKGSLASLEGEIRDVQAIGTHLVYLVEIKNIILSAEGHGLIYFKRRFH
                     PVMLEMEAAI"
     gene            10962..13112
                     /gene="btsT"
                     /locus_tag="ACHOSC_RS12865"
                     /old_locus_tag="pgaptmp_002573"
     CDS             10962..13112
                     /gene="btsT"
                     /locus_tag="ACHOSC_RS12865"
                     /old_locus_tag="pgaptmp_002573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313340.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate/proton symporter BtsT"
                     /protein_id="HGU4548496.1"
                     /translation="MDTKKLFKHIPWVILGIIGAFCLAVVALRRGEHVSALWIVVASV
                     SVYLVAYRYYSLYIAQKVMKLDPTRATPAVINNDGLNYVPTNRYVLFGHHFAAIAGAG
                     PLVGPVLAAQMGYLPGTLWLLAGVVLAGAVQDFMVLFISSRRNGASLGEMIKEEMGPV
                     PGTIALFGCFLIMIIILAVLALIVVKALAESPWGVFTVCSTVPIALFMGIYMRFIRPG
                     RVGEVSVIGIVLLVASIYFGGVIAHDPYWGPALTFKDTTITFALIGYAFVSALLPVWL
                     ILAPRDYLATFLKIGVIVGLALGIVVLNPELKMPAMTQYIDGTGPLWKGALFPFLFIT
                     IACGAVSGFHALISSGTTPKLLANETDARFIGYGAMLMESFVAIMALVAASIIEPGLY
                     FAMNTPPAGLGITMPNLHEMGGENAPIIMAQLKDVTAHAAATVSSWGFVISPEQILQT
                     AKDIGEPSVLNRAGGAPTLAVGIAHVFHKVLPMADMGFWYHFGILFEALFILTALDAG
                     TRSGRFMLQDLLGNFIPFLKKTDSLVAGIIGTAGCVGLWGYLLYQGVVDPLGGVKSLW
                     PLFGISNQMLAAVALVLGTVVLIKMKRTQYIWVTVVPAVWLLICTTWALGLKLFSTNP
                     QMEGFFYMASQYKEKIANGTDLTAQQIANMNHIVVNNYTNAGLSILFLIVVYSIIFYG
                     FKTWLAVRNSDKRTDKETPYVPIPEGGVKISSHH"
     gene            13243..13446
                     /gene="yjiX"
                     /locus_tag="ACHOSC_RS12870"
                     /old_locus_tag="pgaptmp_002574"
     CDS             13243..13446
                     /gene="yjiX"
                     /locus_tag="ACHOSC_RS12870"
                     /old_locus_tag="pgaptmp_002574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbdD/YjiX family protein"
                     /protein_id="HGU4548497.1"
                     /translation="MFGNLGQAKKYLGQAAKMLIGIPDYDNYVEHMKTNHPDKPYMSY
                     EEFFRERQNARYGGDGKGGMRCC"
     gene            13457..14413
                     /gene="yjiA"
                     /locus_tag="ACHOSC_RS12875"
                     /old_locus_tag="pgaptmp_002575"
     CDS             13457..14413
                     /gene="yjiA"
                     /locus_tag="ACHOSC_RS12875"
                     /old_locus_tag="pgaptmp_002575"
                     /EC_number="3.6.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313338.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase"
                     /protein_id="HGU4548498.1"
                     /translation="MNPIAVTLLTGFLGAGKTTLLRHILNEQHGYKIAVIENEFGEVS
                     VDDQLIGDRATQIKTLTNGCICCSRSNELEDALLDLLDNLDKGNIQFDRLVIECTGMA
                     DPGPIIQTFFSHEILCQRYLLDGVIALVDAVHADEQMNQFTIAQSQVGYADRILLTKT
                     DVAGEAEKLRERLARINARAPVYTVTHGDIDLGLLFNTNGFMLEENVVSTKPRFHFIA
                     DKQNDISSIVVELDYPVDISEVSRVMENLLLESADKLLRYKGMLWIDGEPNRLLFQGV
                     QRLYSADWDRPWGDEKPHSTMVFIGIQLPEEEIRAAFAGLRK"
     gene            complement(14500..14691)
                     /locus_tag="ACHOSC_RS12880"
                     /old_locus_tag="pgaptmp_002576"
     CDS             complement(14500..14691)
                     /locus_tag="ACHOSC_RS12880"
                     /old_locus_tag="pgaptmp_002576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000063145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antitoxin"
                     /protein_id="HGU4548499.1"
                     /translation="MSMVKHKRGTLYTLDVQQEAELMALVNKSDEDIDYSDIPASGSE
                     AWSDAKRGNFLPDPGIHAQ"
     gene            complement(14731..>14838)
                     /locus_tag="ACHOSC_RS12885"
                     /old_locus_tag="pgaptmp_002577"
     CDS             complement(14731..>14838)
                     /locus_tag="ACHOSC_RS12885"
                     /old_locus_tag="pgaptmp_002577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001724322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane domain protein"
                     /protein_id="HGU4548500.1"
                     /translation="GKWGVNELKCKKGSDRRDAYSIENVSEYNVPGWLK"
BASE COUNT         3430 a         4075 c         4072 g         3261 t
ORIGIN      
        1 gcaatcggga ataaaagtaa tcattaacat attaatgatt aagatgatcg aatccgagga
       61 gtggtaatga aaggcactat cttcgtcgta gcgttgaacc atcgcagcca acttgatgca
      121 tggcaggaag cgttccagca atccccctac aaagccccgc ctaaaactgc ggtctggttt
      181 attaaaccgc gcaatacggt gattggttgc ggtgaaccga ttccctttcc acagggtgaa
      241 aaggtactga gcggtgcgac tgttgcgctg attgtgggaa aaacggcgac gaaagtacgt
      301 gaagaagatg cggcagagta catcgccgga tatgcgctgg ctaacgacgt cagcctgccg
      361 gaagagagct tttaccgccc ggcaatcaaa gcaaaatgtc gtgatggatt ctgccccatt
      421 ggcgaaaccg tggctctcag caatgtcgat aatctgacca tctataccga gatcaacggg
      481 cgtcctgccg atcactggaa taccgccgat ttacaacgta acgccgcgca gttgctgagc
      541 gccctgagcg aatttgccac actgaatcca ggcgatgcca ttctgctcgg cacgccacag
      601 gcgcgcgtgg aaatacagcc aggcgatcgc gttcgtgttc tcgcagaagg tttcccgccg
      661 ctggaaaatc cggtagtgga cgaacgtgaa gtgaccacgc gcaagagctt cccaacgcag
      721 ccacacccgc acggcacgct gtttgccctc ggcctgaact acgccgacca cgccagcgaa
      781 ctggaattta agccaccgga agaaccgctg gtgttcctga aagcgccaaa taccctcact
      841 ggcgataacc agacctccgt gcgtccaaac aatattgaat acatgcacta tgaagcggag
      901 ctggtggtag ttattggcaa acaggcgcgt aacgtcagcg aagccgatgc catggattat
      961 gtcgcgggct acaccgtgtg taacgactac gccattcgcg actatctgga aaactactac
     1021 cgccctaacc tgcgggtaaa aagccgcgac ggactgacgc cgatgctttc aaccatcgtg
     1081 ccgaaagagg cgatcccgga cccgcataat ctgacccttc gcaccttcgt caacggcgag
     1141 ttacgccagc aaggcaccac cgccgatctg atcttcagcg tgcccttcct gatcgcctac
     1201 ttaagcgaat ttatgaccct gaatccgggc gacatgatcg ccaccggcac accaaaaggc
     1261 ttatctgacg tggtgcctgg cgatgaagta gtggtggaag tagaaggcgt gggctgcctg
     1321 gtgaaccgaa ttgtgagtga ggaaacagcg aaatgaaaaa agtaaatcat tggatcaacg
     1381 gcaaaaatgt tgcaggtaac gactacttcc agaccaccaa tccggcaacg ggtgaagtgc
     1441 tggcggatgt ggcctctggc ggtgaagcgg agatcaatca ggcggtagcg gcagcgaaag
     1501 aggcgttccc gaaatgggcc aatctgccga tgaaagagcg tgcgcgcctg atgcgccgtc
     1561 tgggcgatct gatcgaccag aacgtgccag agatcgccgc gatggaaacc gcggacaccg
     1621 gcctgccgat ccatcagacc aaaaatgtgt tgatcccacg cgcttcccac aactttgaat
     1681 ttttcgcgga agtctgccag cagatgaacg gcaagaccta tccggttgac gacaagatgc
     1741 tcaactacac gctggtgcag ccggtgggcg tttgtgcgct ggtatcgccg tggaacgtac
     1801 cgtttatgac cgccacatgg aaggtcgcgc cgtgtctggc gctgggcaat actgcggtgc
     1861 tgaagatgtc agaactctcc ccgctgaccg ctgaccgcct gggtgagctg gcgctggaag
     1921 ccggtattcc ggcgggcgtg ctgaacgtgg tacagggcta cggcgcaacc gcaggggatg
     1981 cgctggtccg tcatcatgac gtgcgtgccg tgtcgttcac cggcggtacc gccaccgggc
     2041 gcaacatcat gaaaaacgcc gggctgaaaa aatactccat ggaactgggc ggaaaatcgc
     2101 cagtgctgat ttttgaagat gccgatattg aacgcgcgct ggacgccgcc ctgttcacca
     2161 tcttctcgat caacggcgag cgctgcaccg ccggttcgcg catctttatt cagcaaagca
     2221 tctacccgga attcgttaaa cgctttgccg aacgcgccaa ccgtctgcgc gtgggcgatc
     2281 cgaacgatcc gaatacccag gttggggcac ttatcagcca gcaacactgg gaaaaagtct
     2341 ccggctatat ccgtctcggc attgaagaag gcgcaaccct gctggcgggc ggcccggata
     2401 aaccgtctga cctgcctgcg cacctgaaag gcggcaactt cctgcgccca acggtgctgg
     2461 cagacgtaga taaccgtatg cgcgtcgccc aggaagagat tttcgggccg gtcgcctgcc
     2521 tgctgccgtt taaagacgaa gccgaaggct tacgcctggc aaacgacgtg gagtacggcc
     2581 tcgcgtcgta catctggaca caggatgtca gcaaagtgtt acgcctggcg cgtggcattg
     2641 aagcaggcat ggtgttcgtc aatacccaga acgtccgcga cctgcgccag ccgtttggcg
     2701 gcgtaaaagc ctccggcacc gggcgtgaag gcggtgagta cagcttcgaa gtgttcgcgg
     2761 aaatgaagaa cgtctgcatt tccatgggcg accatccaat tccgaaatgg ggagtctgat
     2821 atgggtaagt tagcgttagc cgccaagatc acgcacgtac cgtcgatgta tctctctgaa
     2881 ctaccaggaa aaaaccacgg ttgtcgccag ggtgcgatcg acgggcataa agagatcagc
     2941 aagcgttgcc gggaaatggg cgtcgatacc attatcgttt tcgacaccca ctggctggtc
     3001 aacagtgctt atcacatcaa ctgtgcagac cattttgaag gcgtctacac cagtaacgaa
     3061 ctgccgcatt ttattcgtga catgacctac aactacgagg gcaacccgga gttggggcag
     3121 ctaattgccg atgaagcctt aaagctcggc gtgcgggcaa aagcgcacaa tattcccagc
     3181 ctgaaactgg aatacggcac gctggtgccg atgcgctaca tgaatgaaga caagcacttc
     3241 aaagtggtct ccatttcggc tttctgcacg gttcacgatt ttgccgacag ccgcaagctg
     3301 ggcgaagcga ttctgaaagc gatcgaacag tacgacggca ccgtggcggt ccttgccagc
     3361 ggttcgttat cgcaccgctt tattgacgat cagcgtgcgg aagaagggat gaacagctac
     3421 acccgcgagt tcgaccgcca gatggacgag cgcgtggtga agctgtggcg cgaaggccag
     3481 ttcaaagagt tttgcaatat gctgccggag tacgccgact actgctacgg cgaaggcaat
     3541 atgcacgaca cggtgatgct gctggggatg ctcggctggg ataaatacga cggcaaggtg
     3601 gagtttatca ccgagctatt cccaagctct ggcaccggtc aggttaacgc tgttttcccg
     3661 cttcccgcgt aaggacgttt tatgccgcac tttatcgttg aatgcagtga taacatccgc
     3721 gaagaagccg acctgccggg gttgttcgcc aaagtgaatc cgacgctggc agccacgggt
     3781 atttttccgc tggcgggtat tcgcagccgc gtgcattggg tcgatacctg gcagatggcc
     3841 gacgggcagc atgattatgc cttcgtgcat atgacgttga aaatcggcgc aggtcgcagc
     3901 ctggaaagcc gccagcaggc gggtgaaatg ctgtttgaac tgattaaaac gcacttcgcc
     3961 gccctgatgg agagccgcct gctggcgttg tcgtttgaga ttgaagagct gcatccgacg
     4021 ctgaatttta aacaaaacaa cgtgcacgca ttgtttaagt agcgcgcaga ttgcccggtg
     4081 gcgctgcgct taccgggcct acaaaacccc aaaccgtaca ccgtaggccg gataaggcgc
     4141 agccgcatcc ggcaatgcca caggatatcg ctatgttcga taaacacacc cacaccctga
     4201 tcgcccagcg tctggatcag gcagaaaaac agcgcgaaca gatccgcgcg atctcgctgg
     4261 attacccgga gatcaccatc gaagacgctt acgcggtgca gcgtgaatgg gttcgcctga
     4321 aaatcgccga aggtcgcacg ctgaaaggcc acaaaatcgg cctgacctcg aaagcgatgc
     4381 aggcaagctc gcagatcagc gaaccggatt acggcgcgct gctggatgac atgttcttcc
     4441 acgatggcag cgatatcccg accgatcgct ttatcgtgcc gcgcattgaa gtggagctgg
     4501 ctttcgtgct ggcaaaaccg ctgcgtgggc caaactgcac gctgttcgac gtctacaacg
     4561 ccacggacta cgtgatcccg gcgctggagc tgatcgacgc tcgctgccac aacatcgatc
     4621 cggaaaccca gcgcccgcgc aaagttttcg acaccatttc tgataacgcc gccaacgccg
     4681 gggtgatcct cggaggtcgt ccgatcaaac cagacgaact ggatctgcgt tggatctccg
     4741 ccctgatgta tcgcaatggc gtgattgaag aaaccggcgt cgccgctggc gtgctgaatc
     4801 atccggcaaa cggcgtggcc tggctggcga acaaactcgc accgtatgac gtacaactgg
     4861 aagccgggca aatcattctc ggtggttcgt tcacccgccc ggttccggcg cgtaagggcg
     4921 acaccttcca cgtcgattac ggcaacatgg gttccattag ctgccgcttt gtttaaggag
     4981 agaacgatgg aaaacagctt taaagcggcg ctgaaagcag gccgtccgca gatcggatta
     5041 tggctggggc tgagtagcag ctacagcgca gagttactgg ccggagcagg attcgactgg
     5101 ttgttgatcg acggtgagca cgcgccgaat aacgtgcaaa ccgtgctcac tcagctacag
     5161 gcgattgcgc cctaccccag ccagccggta gtacgtccgt cgtggaacga tccggtgcaa
     5221 atcaaacaac tgctggacgt cggcacacaa accttgctgg tgccgatggt acaaaacgct
     5281 gacgaagccc gtgaagcggt acgcgccacc cgttatcccc ccgccggtat tcgcggtgtg
     5341 ggcagtgcgc tggcccgagc ctcgcgctgg aatcgcattc ctgattacct gcaaaaagcc
     5401 aacgatcaaa tgtgcgtgct ggtgcagatc gaaacgcgtg aggcaatgaa gaacttaccg
     5461 cagattctgg acgtggaagg ggtcgacggc gtgtttatcg gcccggcgga tctgagcgcc
     5521 gatatgggtt atgccggtaa tcctcagcac ccggaagtac aggccgcgat tgagcaggcg
     5581 atcgtgcaga tccgcgaagc gggcaaagcg ccggggatcc tgatcgccaa tgagcaactg
     5641 gcaaaacgct atctggaact gggcgcgctg tttgtcgccg tcggcgttga caccaccctg
     5701 ctcgcccgcg ccgccgaagc gctggcagca cggtttggtg cgcaggctac agcgattaag
     5761 cccgacgtgt attaatgccg gatgcgctgc gcttatccgg cctacactcg caccaaaccg
     5821 taggccggat aaggcgttta cgccgcatcc ggcaaaaagc cgtaccaaat cgcggaccgg
     5881 ataaggcgtt tgtgcgcatc cgccataaac cttgtcgtac cctacaaaaa tcccattaga
     5941 ggaagtaaaa tgagcgacac ctcacctgcc ataccggaga gtatcgatcc ggcgaatcag
     6001 cataaagcgc tgactgccgg acaacaggcg gttattaaga agctatttcg ccgcctgatc
     6061 gtctttctgt tcgtgctgtt tatcttctcg ttccttgatc gcatcaacat cggctttgcc
     6121 ggactcacga tgggacgcga cctcggtctg agcgccacca tgtttggcct cgctaccacc
     6181 ttgttctacg ccgcttacgt catcttcggc attcccagca acattatgct gagtattgtc
     6241 ggtgcacggc gctggatcgc caccatcatg gtgctttggg gcatcgcctc tactgccacc
     6301 atgtttgcca ctggccccac cagcttatac gtactgcgta tactggttgg cattaccgaa
     6361 gccggctttc tgcctggcat tctgctgtat ttaaccttct ggtttccggc ctatttccgc
     6421 gcccgtgcca acgccttgtt tatggtagca atgccggtaa cgacagcgtt gggatcgatc
     6481 gtttccggct acattttgtc gctggatggc gtaatggcat taaaaggctg gcagtggctg
     6541 tttttgctgg aaggcttccc gtcggtatta ctcggcgtca tggtgtggtt ctggcttgat
     6601 gactcaccgg acaaagctaa gtggctgacg aaagaagaca aaaaatgcct gcaagagatg
     6661 atggataacg atcgtctgac gctggttcag ccagagggag ccatcagcca ccacgccatg
     6721 caacaacgca gtatgtggcg ggagatcttc actccggtgg tgatgatgta taccctggcg
     6781 tatttctgcc tgaccaacac acttagtgcg atcagcatct ggacaccgca gatcctgcaa
     6841 agctttaatc agggcagcag taatatcacc atcggcctgc tggccgccgt accgcagatt
     6901 tgtaccattc tcgggatgat ctactggagc cgtcactcag atcgccgcca ggaacgaagg
     6961 catcacaccg cccttcctta tttgttcgct gccgctggtt ggttactggc ttcggcaact
     7021 gatcacaaca tgatccagat gctggggatc attatggctt cgaccggatc attcagcgca
     7081 atggcgattt tctggacaac accggatcag tccatcagcc tgcgggcacg agcgatcggt
     7141 attgcggtga tcaacgccac tggcaacatt ggttcagcgt taagtccgtt tatgatcggc
     7201 tggttgaaag atctgaccgg cagctttaac agtggattgt ggtttgttgc cacgctgctg
     7261 gtgattggtg cggggattat ctgggcgatt ccaatgcagt cctcccgtcc gcgagcgacc
     7321 ccgtaaggaa cgacgatgtg tgaccgtcag attgccaata ttgatatcag caaagagtac
     7381 gatgaaagcc tgggcacgga cgatgtgcat tatcagtcgt tcgcccgcat ggcggccttt
     7441 tttggccgcc atatgctgcc acatcgccac gaacagtact ttcagatgca tttcctcaat
     7501 agcggacaga ttgagctaca gcttgacgat catcgctact cggtggaagc gcccctgttt
     7561 gtcctgacgc cgccgtcagt acctcatgcg tttattacgg agtctgatgc tgacggtcat
     7621 gtattgacgg tacgggaaga tctgatctgg cccctgctgg aagttcttta tccgggcact
     7681 cgggaaacct tcggcctgcc ggggatttgc ctgtcactgg cagataaacc cgacgaactg
     7741 gcggcgctgg aacactattg gcaactgata gagcgggaat cggtagaaca actgcctggt
     7801 cgggaacaca ccctgacgtt actggcacag gcagtgttca ccctactgct gcgtaacgca
     7861 aaactcgacg accatgccgc cagcggaatg cgcggagaat taaaactgtt ccagcgtttt
     7921 catatgctga ttgaaagcca ttttcatcag cactggacag taccggatta cgctaacgaa
     7981 ttgcatatca ccgaatcacg cctcactgac atctgccgcc gctttgccaa ccgtccgcca
     8041 aaacggttga ttttcgacag gcagctgcga gaagccaagc ggctgctgct gttttctgat
     8101 aacgccgtga acaatattgc ctggcaactc ggttttaagg atccggctta ttttgcgcgc
     8161 ttttttaatc gcttagtcgg ttgctcgccc agtgcttatc gcgccaaaaa agtacctgtg
     8221 acgtgaaaat cccttagcct taacgggaaa ccaggcacca cctgctattc ccctttctcc
     8281 gcctggagca aggggattta gtaacgttaa aaatcgctaa aagcgacctc gatcacaaaa
     8341 ccgtacccga tctgaaaagt accagccatt gtcccaaaag tgtcttcccg taatcgcccc
     8401 tgcaagcttc aattgaataa caaaaacaac acacaaaatt aacaatcgta ttccgattaa
     8461 tactgtagag gtcgacatga aaccagaaga tttccgcgcc agtacccaac gtccgttcac
     8521 cggggaagag tatctgaaaa gcctgcagga tggtcgcgag atctatatct atggcgagcg
     8581 agtgaaagac gtcaccactc atccggcatt tcgtaatgcg gcagcgtctg ttgcccaact
     8641 gtacgacgcg ctacacaaac cggagatgca ggactctctg tgctggaaca ccgacaccgg
     8701 tagcggcggc tatacccata aattcttccg cgtggcgaaa agtgccgacg acctgcgcca
     8761 gcaacgcgac gccatcgctg agtggtcacg cctgagctat ggctggatgg gccgtacccc
     8821 agactacaaa gccgctttcg gttgcgcact gggcgcgaat ccgggctttt acggtcagtt
     8881 cgagcagaac gcccgtaact ggtatacccg tattcaggaa actggcctct actttaacca
     8941 cgcgattgtt aacccaccga tcgatcgtca tttgccgacc gataaagtga aagacgttta
     9001 catcaagctg gaaaaagaga ctgacgccgg gattatcgtc agcggtgcga aagtggttgc
     9061 caccaactcg gcgctgactc actacaacat gattggcttc ggctcggcac aagtgatggg
     9121 cgaaaacccg gacttcgcac tgatgttcgt tgcgccaatg gatgccgatg gcgtgaaatt
     9181 aatctcccgc gcctcttatg agatggtcgc gggtgctacc ggctcgccgt acgactaccc
     9241 gctctccagc cgctttgatg agaatgatgc gattctggtg atggataacg tgctgatccc
     9301 atgggaaaac gtactgatct accgcgattt cgatcgctgc cgtcgctgga cgatggaagg
     9361 cggttttgcc cgtatgtatc cgctgcaagc ctgtgtgcgc ctggcagtga aactagactt
     9421 cattacggca ctgctgaaaa aatcactcga atgtaccggc accctggagt tccgtggtgt
     9481 gcaggccgat ctcggtgaag tggtggcgtg gcgcaacacc ttctgggcat tgagtgactc
     9541 gatgtgttca gaagcgacac catgggtcaa cggtgcttat ttaccggatc atgccgcact
     9601 gcaaacctat cgcgtactgg caccaatggc ctacgcgaag atcaaaaaca ttatcgaacg
     9661 caacgttacc agtggcctga tctatctccc ttccagtgcc cgtgacctga acaatccgca
     9721 gatcgaccag tatctggcga agtatgtgcg cggttcgaac ggtatggatc acgtccagcg
     9781 catcaagatc ctcaaactga tgtgggacgc cattggcagc gagtttggtg gtcgtcacga
     9841 actgtatgaa atcaactact ccggtagcca ggatgagatt cgcctgcagt gtctgcgcca
     9901 ggcacaaagc tccggcaata tggacaagat gatggcgatg gttgatcgct gcctgtcgga
     9961 atacgaccag aacggctgga ctgtgccgca cctgcacaac aacgacgata tcaacatgct
    10021 ggataagctg ctgaaataac gcagcaggag gttaagatgc aattagatga acaacgcctg
    10081 cgctttcgtg acgcaatggc cagcctgtcg gcagcggtaa atattatcac caccgagggc
    10141 gacgccggac aatgcggaat tacggcaacg gccgtctgct cgatcacgga tacaccaccg
    10201 tcgctgatgg tgtgcattaa cgccaacagt gcgatgaacc cggtttttca gggcaacggt
    10261 aagttgtgcg tcaacgtcct caatcatgag caggagctga tggcccgcca cttcgcgggc
    10321 atgacaggca tggcgatgga agagcgtttt agcctctcat gctggcaaaa aggtccgctg
    10381 gcacagccgg tgctgaaagg ttcgctggcc agtcttgaag gtgagatccg cgatgtgcag
    10441 gcaattggca cacatctggt gtatctggtg gagattaaaa acatcatcct cagtgcagaa
    10501 ggtcacggac ttatctactt taaacgccgt ttccatccgg tgatgctgga aatggaagct
    10561 gcgatttaag ctccagcccc ttcacgcatt cgtcgaaggg gcatttttat tgtgatctca
    10621 atcacatatc cacccgatga ataattctta cccaaccgct caccgccatt cctgaccgct
    10681 tacatcccta aaataaccac tcagttattt accttacttt acgcgtgcgt aactctggca
    10741 acatcactgc aggatagcgg tcaatttacc tcctcaaacg caacgcaaac ctagaacggc
    10801 ttcggcctat gattaacaaa ttactctatg gattttggtt tgcaggaagg cagcaagtga
    10861 gtgaatcccc gggagcttat aacagtaagt gacaggggtg aacgaacgca gctgccgcac
    10921 ctgtaagcca aaagacgacg agtaaaatgc caggttttac tatggatacg aaaaaactat
    10981 tcaagcacat accctgggtg attctcggaa tcatcggtgc attctgcctc gcggtagttg
    11041 cattacgtcg gggggagcac gtcagcgccc tgtggatcgt ggtcgcctct gtgtcggtgt
    11101 atctggtggc gtatcgctac tacagtctgt atatcgccca gaaggtgatg aaactcgacc
    11161 ccacgcgcgc gacgcctgcg gttattaaca acgacggtct gaactacgtt ccgaccaacc
    11221 gttacgtgtt gtttggtcac cacttcgccg ctatcgccgg tgctggtccg ctggttggtc
    11281 cggttctcgc cgcgcagatg ggctacctgc ccggcacgct gtggctgctg gcgggggtag
    11341 tgctggccgg tgcggtacag gactttatgg tgctgtttat ctcctctcgc cgtaacggcg
    11401 catctctcgg tgagatgatc aaagaagaga tgggaccagt accggggact atcgcgctgt
    11461 ttggctgttt cttaatcatg atcatcatcc ttgccgtcct ggcgctgatt gtggttaaag
    11521 ccctggcaga aagtccgtgg ggtgtcttca ccgtttgctc aaccgtaccg attgcgctgt
    11581 ttatgggtat ctacatgcgc tttatccgtc cggggcgtgt gggtgaagtc tctgtaatcg
    11641 gtatcgtgct gctggttgcc tctatctact tcggtggcgt gattgctcac gatccgtact
    11701 ggggtccggc actgaccttt aaagacacca ccattacctt cgcgctgatt ggctatgcgt
    11761 ttgtttccgc actgctgccg gtgtggctga tcctcgcacc gcgcgactat ctggcaacct
    11821 tcctgaaaat cggcgttatc gtcggcctgg cgttgggtat cgtggtgctg aacccggaac
    11881 tgaaaatgcc tgcgatgacc cagtacattg acggtactgg cccgctgtgg aaaggcgctc
    11941 tgttcccgtt cctgttcatc accatcgcct gtggtgcggt atctggcttc cacgcgctga
    12001 tctcttccgg tacgacgccg aaactgctgg ctaacgaaac cgacgcgcgt ttcattggct
    12061 acggcgcaat gctgatggag tccttcgtgg cgattatggc gctggttgca gcgtccatca
    12121 tcgaaccggg tctttacttc gcgatgaaca ccccgcctgc gggccttggc atcaccatgc
    12181 ctaacctgca tgaaatgggt ggcgagaacg cgccgatcat catggcgcag ctgaaagacg
    12241 ttaccgcaca cgcggcggcg accgtcagct cctggggctt cgtgatttcg ccagagcaga
    12301 tcctgcaaac cgcgaaagac attggtgagc cttctgtcct gaaccgtgca ggtggtgcgc
    12361 cgacactggc ggtaggtatc gcgcacgtgt tccacaaagt gctgccgatg gctgacatgg
    12421 gcttctggta tcacttcggt attctgttcg aagccctgtt catcctgacc gcgctggatg
    12481 cgggtacccg ttctggccgc tttatgctgc aggacctgct gggtaacttc atcccgttcc
    12541 tgaaaaaaac cgactctctg gttgctggta tcatcggtac tgcgggctgt gtgggtctgt
    12601 ggggctacct gctgtatcag ggcgtggtcg atccgctggg cggcgttaag agcctgtggc
    12661 cgctgttcgg tatctccaac cagatgctgg cagccgtagc gctggtactg ggcaccgttg
    12721 tgctgattaa gatgaagcgc acccaataca tctgggtaac tgttgttccg gctgtatggc
    12781 tgcttatctg caccacctgg gcgctgggcc tgaaactgtt cagcaccaac ccgcagatgg
    12841 aaggcttctt ctacatggca agccagtaca aagagaagat tgctaacggt actgacctga
    12901 cggcacagca gattgccaac atgaaccaca tcgttgtgaa caactacacc aacgcgggcc
    12961 tgagtattct gttcctgatt gtggtgtaca gcatcatctt ctacggtttc aaaacctggc
    13021 ttgcggtgcg taacagcgac aaacgtactg acaaagaaac accgtacgtt ccaatcccgg
    13081 aaggcggcgt gaagatctct tcgcaccact aaccggttgc cggatgcgtt gcgcgtatcc
    13141 ggcctacaaa gtgtctatcg taggccggat aaagcgttta cgccgcatcc ggcaaatccc
    13201 tagcccggac cttccgggct ttacgcatat ctggaaggca aaatgtttgg taacttagga
    13261 caggcaaaaa aatatctcgg ccaggcggcg aagatgttga ttggcattcc agactacgac
    13321 aactacgtcg agcatatgaa gaccaaccat cccgacaagc cgtacatgag ctatgaagaa
    13381 ttcttccgcg aacgccagaa cgcgcgctac ggcggcgatg gtaaaggcgg tatgcgctgt
    13441 tgttaatgga gagagcatga acccgattgc agttacccta ctcaccggtt ttttaggcgc
    13501 gggcaaaacc accctgctgc gccatattct taacgaacaa cacggctaca agattgccgt
    13561 gattgaaaac gaattcggcg aagtctccgt tgatgatcaa ctgattggcg accgcgccac
    13621 gcagatcaaa acgctgacca acggctgcat ctgctgttcg cgctccaacg agctggaaga
    13681 cgcactactc gacctgctgg ataatctcga caagggcaat attcagttcg accgtctggt
    13741 cattgaatgc accggcatgg ccgatcccgg tccgattatt cagacctttt tctcccatga
    13801 gattttatgc cagcgttacc tgctggacgg cgtgattgcg ctggtggatg cggtacatgc
    13861 cgatgagcag atgaaccagt tcaccatcgc ccagtcccag gttggctacg ccgaccgtat
    13921 tctgctgacc aaaaccgacg tcgcaggcga agcagaaaaa ctgcgtgaac gcctggcgcg
    13981 catcaacgcc cgtgcaccgg tctacaccgt cacccacggc gacatcgacc tgggcctgct
    14041 attcaacacc aacggtttta tgctggaaga gaacgtcgtc agcaccaaac cgcgcttcca
    14101 ctttatcgcg gataaacaaa acgatatttc gtcgattgtg gtggaactgg attacccggt
    14161 agatatcagc gaagtttccc gggtgatgga aaacctgctg ctggaatcgg cagataaact
    14221 gctgcgttac aaagggatgc tgtggattga cggcgaacct aaccgcctgc tattccaggg
    14281 cgtccagcgc ctctacagcg ccgactggga ccgcccgtgg ggcgatgaaa aaccgcatag
    14341 cacgatggtg tttatcggga ttcagctgcc ggaggaagag attcgggcgg cgtttgcggg
    14401 gttgaggaag taatcgtgaa aggttgagtg taaagcagct caatgcccca tttagcagtg
    14461 aggggggaaa cagaacgacg tgctcccctc cgctaagttt tattgcgcgt gaatacctgg
    14521 atctggcaaa aaattgccgc gtttggcgtc agaccatgct tcgctaccag aagcaggaat
    14581 gtcgctgtaa tcaatgtctt cgtcggattt attaaccaaa gccatgagtt cagcttcttg
    14641 ctgcacatca agtgtgtaga gtgtgccccg tttatgttta accatgctca tttatgacca
    14701 gaacctttac gatttaatta taaagatgac ttatttcagc catccaggca cgttatattc
    14761 tgagacattc tcaatggaat atgcatctct cctgtcagaa cctttcttac acttcaactc
    14821 attgaccccc catttacc
//
LOCUS       DAZKHF020000104        14835 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2201S31241
            SAMN43267800-rid25106413.denovo.105, whole genome shotgun sequence.
ACCESSION   DAZKHF020000104 DAZKHF020000000
VERSION     DAZKHF020000104.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267800
            Sequence Read Archive: SRR30307263
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 14835)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14835)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 14835)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350581.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350581.2
            Genome Coverage       :: 151x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:21:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,652
            CDSs (total)                      :: 5,557
            Genes (coding)                    :: 5,320
            CDSs (with protein)               :: 5,320
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 237
            CDSs (without protein)            :: 237
            Pseudo Genes (ambiguous residues) :: 0 of 237
            Pseudo Genes (frameshifted)       :: 102 of 237
            Pseudo Genes (incomplete)         :: 130 of 237
            Pseudo Genes (internal stop)      :: 52 of 237
            Pseudo Genes (multiple problems)  :: 41 of 237
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14835
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267800-rid25106413.denovo.105"
                     /serotype="O 26:H11"
                     /isolate="2201S31241"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-01"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            75..593
                     /gene="yfiR"
                     /locus_tag="ACHOSC_RS12890"
                     /old_locus_tag="pgaptmp_002578"
     CDS             75..593
                     /gene="yfiR"
                     /locus_tag="ACHOSC_RS12890"
                     /old_locus_tag="pgaptmp_002578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfiR family protein"
                     /protein_id="HGU4548501.1"
                     /translation="MRFSHRLFLLLILLLTGAPILAQEPSDVAKNVRMMVSGIVSYTR
                     WPALSGPPKLCIFSSSRFSTALQENAATSLPYLPVIIHTQQEAMISGCNGFYFGNESP
                     TFQMELTEQYPSKALLLIAEQNTECIIGSAFCLIIHNNDVRFAVNLDALSRSGVKVNP
                     DVLMLARKKNDG"
     gene            583..1809
                     /gene="dgcN"
                     /locus_tag="ACHOSC_RS12895"
                     /old_locus_tag="pgaptmp_002579"
     CDS             583..1809
                     /gene="dgcN"
                     /locus_tag="ACHOSC_RS12895"
                     /old_locus_tag="pgaptmp_002579"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase DgcN"
                     /protein_id="HGU4548502.1"
                     /translation="MMDNDNSLNKRPTFKRALRNISMTSIFITMMLIWLLLSVTSVLT
                     LKQYAQKNLALTAATMTYSLEAAVVFADGPAATETLAALGQQGQFSTAEVRDKQQNIL
                     ASWHYTRKDPGDTFSNFISHWLFPAPIIQPIRHNGETIGEVRLTARDSSISHFIWFSL
                     AVLTGCILLASGIAITLTRHLHNGLVEALKNITDVVHDVRSNRNFSRRVSEERIAEFH
                     RFALDFNSLLDEMEEWQLRLQAKNAQLLRTALHDPLTGLANRAAFRSGINTLMNNSDA
                     RKTSALLFLDGDNFKYINDTWGHATGDRVLIEIAKRLAEFGGLRHKAYRLGGDEFAMV
                     LYDVQSESEVQQICSALTQIFNLPFDLHNGHQTTMTLSIGYAMTIEHASAKKLQELAD
                     HNMYQAKHQRAEKLVR"
     gene            1825..2307
                     /gene="yfiB"
                     /locus_tag="ACHOSC_RS12900"
                     /old_locus_tag="pgaptmp_002580"
     CDS             1825..2307
                     /gene="yfiB"
                     /locus_tag="ACHOSC_RS12900"
                     /old_locus_tag="pgaptmp_002580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HGU4548503.1"
                     /translation="MIKHLVAPLIFTSLILTGCQSPQGKFTPEQVAAMQSYGFTESAG
                     DWSLGLSDAILFAKNDYKLLPESQQQIQTMAAKLASTGLTHARMDGHTDNYGEDSYNE
                     GLSLKRANVVADAWAMGGQIPRSNLTTQGLGKKYPIASNKTAQGRAENRRVAVVITTP
                     "
     gene            complement(2384..2731)
                     /gene="rplS"
                     /locus_tag="ACHOSC_RS12905"
                     /old_locus_tag="pgaptmp_002581"
     CDS             complement(2384..2731)
                     /gene="rplS"
                     /locus_tag="ACHOSC_RS12905"
                     /old_locus_tag="pgaptmp_002581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012768745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L19"
                     /protein_id="HGU4548504.1"
                     /translation="MSNIIKQLEQEQMKQDVPSFRPGDTVEVKVWVVEGSKKRLQAFE
                     GVVIAIRNRGLHSAFTVRKISNGEGVERVFQTHSPVVDSISVKRRGAVRKAKLYYLRE
                     RTGKAARIKERLN"
     gene            complement(2773..3540)
                     /gene="trmD"
                     /locus_tag="ACHOSC_RS12910"
                     /old_locus_tag="pgaptmp_002582"
     CDS             complement(2773..3540)
                     /gene="trmD"
                     /locus_tag="ACHOSC_RS12910"
                     /old_locus_tag="pgaptmp_002582"
                     /EC_number="2.1.1.228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052906 - tRNA
                     (guanine(37)-N1)-methyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0030488 - tRNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(37)-N1)-methyltransferase TrmD"
                     /protein_id="HGU4548505.1"
                     /translation="MWIGIISLFPEMFRAITDYGVTGRAVKNGLLSIQSWSPRDFTHD
                     RHRTVDDRPYGGGPGMLMMVQPLRDAIHAAKAAAGEGAKVIYLSPQGRKLDQAGVSEL
                     ATNQKLILVCGRYEGIDERVIQTEIDEEWSIGDYVLSGGELPAMTLIDSVSRFIPGVL
                     GHEASATEDSFAEGLLDCPHYTRPEVLEGMEVPPVLLSGNHAEIRRWRLKQSLGRTWL
                     RRPELLENLALTEEQARLLAEFKTEHAQQQHKHDGMA"
     gene            complement(3571..4119)
                     /gene="rimM"
                     /locus_tag="ACHOSC_RS12915"
                     /old_locus_tag="pgaptmp_002583"
     CDS             complement(3571..4119)
                     /gene="rimM"
                     /locus_tag="ACHOSC_RS12915"
                     /old_locus_tag="pgaptmp_002583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000043333.1"
                     /note="Essential for efficient processing of 16S rRNA;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimM"
                     /protein_id="HGU4548506.1"
                     /translation="MSKQLTAQAPVDPIVLGKMGSSYGIRGWLRVFSSTEDAESIFDY
                     QPWFIQKAGQWQQVQLESWKHHNQDMIIKLKGVDDRDAANLLTNCEIVVDSSQLPQLE
                     EGDYYWKDLMGCQVVTTEGYDLGKVVDMMETGSNDVLVIKANLKDAFGIKERLVPFLD
                     GQVIKKVDLTTRSIEVDWDPGF"
     gene            complement(4138..4386)
                     /gene="rpsP"
                     /locus_tag="ACHOSC_RS12920"
                     /old_locus_tag="pgaptmp_002584"
     CDS             complement(4138..4386)
                     /gene="rpsP"
                     /locus_tag="ACHOSC_RS12920"
                     /old_locus_tag="pgaptmp_002584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000256453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S16"
                     /protein_id="HGU4548507.1"
                     /translation="MVTIRLARHGAKKRPFYQVVVADSRNARNGRFIERVGFFNPIAS
                     EKEEGTRLDLDRIAHWVGQGATISDRVAALIKEVNKAA"
     gene            complement(4635..5996)
                     /gene="ffh"
                     /locus_tag="ACHOSC_RS12925"
                     /old_locus_tag="pgaptmp_002585"
     CDS             complement(4635..5996)
                     /gene="ffh"
                     /locus_tag="ACHOSC_RS12925"
                     /old_locus_tag="pgaptmp_002585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005130432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0048501 - signal recognition particle,
                     plasma membrane targeting [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005048 - signal sequence binding
                     [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle protein"
                     /protein_id="HGU4548508.1"
                     /translation="MFDNLTDRLSRTLRNISGRGRLTEDNVKDTLREVRMALLEADVA
                     LPVVREFINRVKEKAVGHEVNKSLTPGQEFVKIVRNELVAAMGEENQTLNLAAQPPAV
                     VLMAGLQGAGKTTSVGKLGKFLREKHKKKVLVVSADVYRPAAIKQLETLAEQVGVDFF
                     PSDVGQKPVDIVNAALKEAKLKFYDVLLVDTAGRLHVDEAMMDEIKQVHASINPVETL
                     FVVDAMTGQDAANTAKAFNEALPLTGVVLTKVDGDARGGAALSIRHITGKPIKFLGVG
                     EKTEALEPFHPDRIASRILGMGDVLSLIEDIESKVDRAQAEKLASKLKKGDGFDLNDF
                     LEQLRQMKNMGGMASLMGKLPGMGQIPDNVKSQMDDKVLVRMEAIINSMTIKERAKPE
                     IIKGSRKRRIAAGCGMQVQDVNRLLKQFDDMQRMMKKMKKGGMAKMMRSMKGMMPPGF
                     PGR"
     gene            6163..6954
                     /gene="ypjD"
                     /locus_tag="ACHOSC_RS12930"
                     /old_locus_tag="pgaptmp_002586"
     CDS             6163..6954
                     /gene="ypjD"
                     /locus_tag="ACHOSC_RS12930"
                     /old_locus_tag="pgaptmp_002586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417102.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="inner membrane protein YpjD"
                     /protein_id="HGU4548509.1"
                     /translation="MPVFALLALVAYSVSLALIVPGLLQKNGGWRRMAIISAVIALVC
                     HAIALEARILPDGDSGQNLSLLNVGSLVSLMICTVMTIVASRNRGWLLLPIVYAFALI
                     NLALATFMPNEYITHLEATPGMLVHIGLSLFSYATLIIAALYALQLAWIDYQLKNKKL
                     AFNQEMPPLMSIERKMFHITQIGVVLLTLTLCTGLFYMHNLFSMENIDKAVLSIVAWF
                     VYIVLLWGHYHEGWRGRRVVWFNVAGAVILTLAYFGSRIVQQLIS"
     gene            6975..8261
                     /gene="yfjD"
                     /locus_tag="ACHOSC_RS12935"
                     /old_locus_tag="pgaptmp_002587"
     CDS             6975..8261
                     /gene="yfjD"
                     /locus_tag="ACHOSC_RS12935"
                     /old_locus_tag="pgaptmp_002587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026171.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyC/CorC family transporter"
                     /protein_id="HGU4548510.1"
                     /translation="MEHISTTTLIIILIIMVVISAYFSGSETGMMTLNRYRLRHMAKQ
                     GNRSAKRVEKLLRKPDRLISLVLIGNNLVNILASALGTIVGMRLYGDAGVAIATGVLT
                     FVVLVFAEVLPKTIAALYPEKVAYPSSFLLAPLQILMMPLVWLLNAITRMLMRMMGIK
                     TDIVVSGSLSKEELRTIVHESRSQISRRNQDMLLSVLDLEKMTVDDIMVPRSEIIGID
                     INDDWKSILRQLSHSPHGRIVLYRDSLDDAISMLRVREAWRLMSEKKEFTKETMLRAA
                     DEIYFVPEGTPLSTQLVKFQRNKKKVGLVVNEYGDIQGLVTVEDILEEIVGDFTTSMS
                     PTLAEEVTPQNDGSVIIDGTANVREINKAFNWHLPEDDARTVNGVILEALEEIPVAGT
                     RVRIGEYDIDILDVQDNMIKQVKVFPVKPLRESVAE"
     gene            complement(8316..8909)
                     /gene="grpE"
                     /locus_tag="ACHOSC_RS12940"
                     /old_locus_tag="pgaptmp_002588"
     CDS             complement(8316..8909)
                     /gene="grpE"
                     /locus_tag="ACHOSC_RS12940"
                     /old_locus_tag="pgaptmp_002588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide exchange factor GrpE"
                     /protein_id="HGU4548511.1"
                     /translation="MSSKEQKTPEGQAPEEIIMDQHEEIEAVEPEASAEQVDPRDEKI
                     ANLEAQLAEAQTRERDGILRVKAEMENLRRRTELDIEKAHKFALEKFINELLPVIDSL
                     DRALEVADKANPDMSAMVEGIELTLKSMLDVVRKFGVEVIAETNVPLDPNVHQAIAMV
                     ESDDVAPGNVLGIMQKGYTLNGRTIRAAMVTVAKAKA"
     gene            9032..9910
                     /gene="nadK"
                     /locus_tag="ACHOSC_RS12945"
                     /old_locus_tag="pgaptmp_002589"
     CDS             9032..9910
                     /gene="nadK"
                     /locus_tag="ACHOSC_RS12945"
                     /old_locus_tag="pgaptmp_002589"
                     /EC_number="2.7.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020239499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003951 - NAD+ kinase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(+) kinase"
                     /protein_id="HGU4548512.1"
                     /translation="MNNHFKCIGIVGHPRHPTALTTHEMLYRWLCTKGYEVIVEQQIA
                     HELQLKNVKTGTLAEIGQLADLAVVVGGDGNMLGAARTLARYDIKVIGINRGNLGFLT
                     DLDPDNAQQQLADVLEGHYISEKRFLLEAQVCQQDCQKRISTAINEVVLHPGKVAHMI
                     EFEVYIDEIFAFSQRSDGLIISTPTGSTAYSLSAGGPILTPSLDAITLVPMFPHTLSA
                     RPLVINSSSTIRLRFSHRRNDLEISCDSQIALPIQEGEDVLIRRCDYHLNLIHPKDYS
                     YFNTLSTKLGWSKKLF"
     gene            9996..11657
                     /gene="recN"
                     /locus_tag="ACHOSC_RS12950"
                     /old_locus_tag="pgaptmp_002590"
     CDS             9996..11657
                     /gene="recN"
                     /locus_tag="ACHOSC_RS12950"
                     /old_locus_tag="pgaptmp_002590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecN"
                     /protein_id="HGU4548513.1"
                     /translation="MLAQLTISNFAIVRELEIDFHSGMTVITGETGAGKSIAIDALGL
                     CLGGRAEADMVRTGAARADLCARFSLKDTPAALRWLEENQLEDGHECLLRRVISSDGR
                     SRGFINGTAVPLSQLRELGQLLIQIHGQHAHQLLTKPEHQKFLLDGYANETSLLQEMT
                     ARYQLWHQSCRDLAHHQQLSQERAARAELLQYQLKELNEFNPQPGEFEQIDEEYKRLA
                     NSGQLLTTSQNALALMADGEDANLQSQLYTAKQLVSELIGMDSKLSGVLDMLEEATIQ
                     IAEASDELRHYCDRLDLDPNRLFELEQRISKQISLARKHHVSPEALPQYYQSLLEEQQ
                     QLDDQADSQETLALAVTKHHQQALEIARALHQQRQQYAEELAQLITDSMHALSMPHGQ
                     FTIDVKFDEHHLGADGADRIEFRVTTNPGQPMQPIAKVASGGELSRIALAIQVITARK
                     METPALIFDEVDVGISGPTAAVVGKLLRQLGESTQVMCVTHLPQVAGCGHQHYFVSKE
                     TDGAMTETHMQSLNKKARLQELARLLGGSEVTRNTLANAKELLAA"
     gene            11806..12147
                     /gene="bamE"
                     /locus_tag="ACHOSC_RS12955"
                     /old_locus_tag="pgaptmp_002591"
     CDS             11806..12147
                     /gene="bamE"
                     /locus_tag="ACHOSC_RS12955"
                     /old_locus_tag="pgaptmp_002591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417107.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamE"
                     /protein_id="HGU4548514.1"
                     /translation="MRCKTLTAAAAVLLMLTAGCSTLERVVYRPDINQGNYLTANDVS
                     KIRVGMTQQQVAYALGTPLMSDPFGTNTWFYVFRQQPGHEGVTQQTLTLTFNSSGVLT
                     NIDNKPALSGN"
     gene            complement(12209..12499)
                     /gene="yfjF"
                     /locus_tag="ACHOSC_RS12960"
                     /old_locus_tag="pgaptmp_002592"
     CDS             complement(12209..12499)
                     /gene="yfjF"
                     /locus_tag="ACHOSC_RS12960"
                     /old_locus_tag="pgaptmp_002592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417108.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RnfH family protein"
                     /protein_id="HGU4548515.1"
                     /translation="MPGKIAVEVAYALPEKQYLQRVTLQEGATVEEAIRASGLLELRT
                     DIDLTKNKVGIYSRPAKLSDSVHDGDRVEIYRPLIADPKELRRQRAEKSANK"
     gene            complement(12489..12926)
                     /gene="ratA"
                     /locus_tag="ACHOSC_RS12965"
                     /old_locus_tag="pgaptmp_002593"
     CDS             complement(12489..12926)
                     /gene="ratA"
                     /locus_tag="ACHOSC_RS12965"
                     /old_locus_tag="pgaptmp_002593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010376629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system toxin RatA"
                     /protein_id="HGU4548516.1"
                     /translation="MPQISRTALVPYSAEQMYQLVNDVQSYPQFLPGCTGSRILESTP
                     GQMTAAVDVSKAGISKTFTTRNQLTSNQSILMNLVDGPFKKLIGGWKFTPLSQEACRI
                     EFHLDFEFTNKLIELAFGRVFKELASNMVQAFTVRAKEVYSAR"
     gene            13097..13579
                     /gene="smpB"
                     /locus_tag="ACHOSC_RS12970"
                     /old_locus_tag="pgaptmp_002594"
     CDS             13097..13579
                     /gene="smpB"
                     /locus_tag="ACHOSC_RS12970"
                     /old_locus_tag="pgaptmp_002594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SsrA-binding protein SmpB"
                     /protein_id="HGU4548517.1"
                     /translation="MTKKKAHKPGSATIALNKRARHEYFIEEEFEAGLALQGWEVKSL
                     RAGKANISDSYVLLRDGEAFLFGANITPMAVASTHVVCDPTRTRKLLLNQRELDSLYG
                     RVNREGYTVVALSLYWKNAWCKVKIGVAKGKKQHDKRSDIKEREWQVDKARIMKNAHR
                     "
     gene            13794..14156
                     /gene="ssrA"
                     /locus_tag="ACHOSC_RS12975"
                     /old_locus_tag="pgaptmp_002595"
     tmRNA           13794..14156
                     /gene="ssrA"
                     /locus_tag="ACHOSC_RS12975"
                     /old_locus_tag="pgaptmp_002595"
                     /product="transfer-messenger RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00023"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00023"
     gene            14427..14675
                     /locus_tag="ACHOSC_RS12980"
                     /old_locus_tag="pgaptmp_002596"
                     /pseudo
     CDS             14427..14675
                     /locus_tag="ACHOSC_RS12980"
                     /old_locus_tag="pgaptmp_002596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310966.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DinI-like family protein"
BASE COUNT         3775 a         3908 c         3491 g         3661 t
ORIGIN      
        1 gaaaaaatga tattttaatg agcattctga aaaaatatcg atgcatttcg agcgaagatg
       61 gtggggatcc ctgaatgcgt ttttctcacc gactgttctt acttctgatt ctcctgctga
      121 cgggagcacc aatactcgct caggagcctt ctgacgttgc gaaaaacgtg cgtatgatgg
      181 tttccggtat tgtcagttac acccgctggc ctgcgctatc agggccacca aagttatgta
      241 tcttttcatc ttcacgtttt agtaccgccc tacaagagaa tgccgcgaca tctttgcctt
      301 atctgcctgt catcattcac actcaacaag aggcgatgat ttcaggctgc aatggttttt
      361 attttggcaa cgagtcaccg acattccaga tggaattaac cgaacaatat ccgtcaaagg
      421 cgttattatt aattgccgaa caaaataccg agtgcattat tggtagcgcc ttttgtctga
      481 tcatccacaa taatgacgtc agatttgccg taaacctgga tgccttatcg cgtagcggtg
      541 taaaggtcaa cccggatgtc ttaatgctcg cacggaagaa aaatgatgga taacgataat
      601 tctcttaata agcgccccac gtttaaaaga gcattacgca acatcagtat gaccagcata
      661 tttatcacta tgatgctgat ctggttgctg ctttccgtga cctcggtgct gaccctgaaa
      721 cagtacgcgc aaaaaaacct ggcactgaca gcagcaacaa tgacttacag tctggaagca
      781 gctgtcgttt ttgccgatgg ccctgcagca actgaaacac tggcagcgct gggccagcaa
      841 gggcaatttt caactgcaga agtacgtgat aagcagcaaa atattctggc gtcctggcat
      901 tacacccgta aggatccagg cgatactttc agcaatttca taagccactg gctcttcccc
      961 gcccccatca ttcagccgat tcgtcacaat ggtgaaacca ttggcgaagt acgcttaacc
     1021 gctcgcgaca gttcaatcag ccattttatc tggttttcgc tcgccgtact gaccggttgt
     1081 attctgctgg catcaggcat cgcaattacc ctcacccgcc atttgcacaa tggcctggta
     1141 gaagcgctga aaaatatcac cgatgtcgta catgatgtgc gttccaaccg caatttttcc
     1201 cgacgagttt cggaagaacg tatcgctgag tttcaccgct tcgctctcga cttcaacagt
     1261 ctgctggatg aaatggaaga gtggcagctt cgtttacagg ccaaaaatgc gcagcttctg
     1321 cgtaccgcgc tacatgaccc attaaccggg ctggctaacc gcgcagcgtt tcgtagcggc
     1381 atcaatacgt tgatgaacaa ttccgatgcc cgaaaaacgt cggcgttact atttcttgat
     1441 ggcgataatt tcaaatatat caatgatacc tggggtcatg cgacgggcga tagagtcttg
     1501 attgaaatcg caaaacggtt agctgaattt ggcgggctgc gacataaagc ataccgcctg
     1561 ggcggcgatg aattcgctat ggtgctctat gatgtacagt cggaatctga agtgcagcag
     1621 atatgctcag cactgacaca aatctttaat ctcccgtttg atcttcataa tggccatcag
     1681 accaccatga cattaagcat tggttacgcg atgaccattg agcacgcctc tgcgaaaaaa
     1741 ttacaagagc ttgccgatca caatatgtat caggccaaac accagcgtgc cgaaaagctg
     1801 gtgagataac aaggatatat cgatatgata aagcacctgg tagcacccct gattttcacc
     1861 tcactaatac tgactggctg ccagtcccct cagggaaagt ttactcctga gcaagtcgcc
     1921 gctatgcaat cttatggatt tactgaatcc gccggcgact ggtcgctggg cttatcagat
     1981 gccattctgt tcgcaaaaaa tgactacaaa ttgctcccgg aaagccagca acagatccaa
     2041 accatggcag ccaaattggc ctcgacaggg ctaacacatg cccgtatgga tggacacacc
     2101 gataactatg gtgaagacag ttacaacgaa ggcttatcat tgaaacgggc gaatgtcgtg
     2161 gccgatgcat gggctatggg tggacaaatt ccgcgcagca atctcaccac acagggttta
     2221 ggaaaaaaat atcccatagc cagtaacaag accgcccagg gccgcgccga gaaccgccgc
     2281 gtcgcagtgg tgattactac cccttaaaac accagcaaac agataaaaaa agggccagcc
     2341 aattggccag cccttcttaa caggatgtcg cttaagcgaa atcttagtta agacgctctt
     2401 tgatacgagc agccttacca gtacgctcac gcaggtagta cagtttagct ttacgaacag
     2461 caccacgacg tttgacagaa atgctgtcaa ctaccggaga gtgagtctgg aagacacgct
     2521 caacgccttc gccgttggaa attttacgaa cagtgaatgc agagtgcaga ccgcggttac
     2581 gaatagcgat aaccacgccc tcgaatgcct gcagacgttt tttggaacct tcaacaaccc
     2641 atactttcac ttccacggta tcacccggac ggaaggaagg tacgtcctgc ttcatctgct
     2701 cttgttcaag ttgcttaata atgttgctca taatttaatc tcttatcctg ggtaaactga
     2761 tatctcgggg gcttacgcca tcccatcatg tttatgttgc tgttgtgcgt gttccgtttt
     2821 gaactccgcc agcaaccttg cttgctcttc agtcagagcc aggttttcca gaagttcagg
     2881 tcttctaagc caggtacggc ccagcgactg tttcaaacgc cagcgacgta tctcggcatg
     2941 gttgcccgac agtaacactg gcggaacttc catcccttct aacacctcag gccgcgtata
     3001 gtgcgggcaa tccagcaatc cttcagcaaa ggaatcttcc gttgccgagg cttcatgtcc
     3061 cagtactccc ggaataaacc gggaaacgga gtcaatcagc gtcattgctg gtaactcacc
     3121 accactgaga acgtaatcgc cgattgacca ttcttcgtca atttcggttt ggatcacgcg
     3181 ctcatctata ccttcgtagc gaccgcacac cagaatcaat ttttgattcg ttgccagttc
     3241 gctgacgccc gcttgatcaa gcttgcgtcc ctgtggtgac agataaatca cctttgcgcc
     3301 ttcacccgcc gcggcttttg ctgcatgaat ggcgtcccgc aagggttgca ccatcattaa
     3361 catccccggt ccgccgccgt aaggacgatc gtccacggta cggtgccggt catgcgtgaa
     3421 gtcgcgagga ctccaactct ggatgctcag caggccattt ttaactgccc ggccagttac
     3481 cccgtaatcg gtaattgcgc ggaacatttc aggaaacagg ctaattatgc caatccacat
     3541 agcgccgtct tttaccgttt atccggtggt ttaaaaacca ggatcccaat ctacttcgat
     3601 tgaacgagta gtgagatcga ctttcttgat aacctgccca tcgaggaacg gtacgagacg
     3661 ttccttgata ccaaacgcat ctttcaggtt tgccttaatg acgagaacgt cattagatcc
     3721 ggtttccatc atatcgacga ctttaccgag atcgtagcct tcagtggtta ctacctggca
     3781 gcccatcagg tctttccagt agtagtcccc ctcttcaagc tgaggcagct gcgatgaatc
     3841 cacgacaatt tcacaattcg tcagcaggtt cgccgcatca cgatcgtcaa cgcctttcag
     3901 cttgatgatc atgtcctgat tgtggtgctt ccagctttcc agctggactt gctgccactg
     3961 acccgccttc tggataaacc agggctgata gtcaaaaatg ctttcggcgt cttcggtgga
     4021 agaaaacact ctgagccacc cacgaatacc gtaagacgaa cccatttttc ccaaaacgat
     4081 gggatcaaca ggtgcttgcg cggtgagttg tttgctcatc atgaccaccg tgacagatta
     4141 agctgctttg tttacttctt tgatcagcgc agcaacgcga tcagaaatag ttgcgccctg
     4201 gccaacccag tgagcgatgc gatccagatc caggcgagtg ccttcttctt tttcgctagc
     4261 gattgggttg aagaaaccaa cgcgctcgat gaagcgaccg ttgcgtgcat tacggctgtc
     4321 agcgacaaca acctggtaga acggacgctt tttagcgccg tgacgtgcta aacgaatagt
     4381 taccataaca tcctcttgtg tgaataaaac aaccggaccc catcgaggaa cggagtccgg
     4441 tgtcatatta aaagcccgaa aattttactc atttttgcgg gaattgcaat caacagttgc
     4501 taactctgct gtaaaaggcc gtcggcggtg cagccagttt ggtgccggag tgcgcgcagt
     4561 caccggagcg tacacgcagt acgtgaggat gacgagcaca tcccggtgcc aaaatggcaa
     4621 acaagccagg ccgattagcg accagggaag cctgggggca tcataccctt catgcttctc
     4681 atcatcttcg ccattccgcc tttcttcatt ttcttcatca tgcgctgcat gtcgtcgaac
     4741 tgcttcagaa ggcggttcac gtcctgcacc tgcataccgc aaccggcagc gatacgacgc
     4801 ttacgcgaac ctttgatgat ttctggctta gcgcgctctt ttatcgtcat cgagttgatg
     4861 atggcttcca tacgcaccag cactttatcg tccatctgcg acttaacatt atccgggatc
     4921 tgccccatgc ccggcagctt gcccatcagg ctagccatgc cgcccatatt tttcatctgg
     4981 cgcagttgct caagaaaatc gttgagatcg aagccgtcgc cttttttcag cttgctggct
     5041 aatttctctg cctgcgcgcg gtcaactttg ctttcgatat cttcgatcag cgacagtacg
     5101 tcgcccatgc cgagaatacg cgacgcgatg cggtccggat ggaacggctc cagcgcctca
     5161 gttttctcgc caacaccgag aaacttgatc ggtttgccag tgatgtgacg aatagagagc
     5221 gccgcaccgc cgcgggcatc gccgtccact ttggtcaaca ctacgccggt aagcggtaac
     5281 gcttcattga atgcttttgc cgtattggcc gcatcctgac cggtcatggc gtcaaccaca
     5341 aacagggttt caaccgggtt aatcgacgca tggacttgtt tgatctcgtc catcatcgct
     5401 tcgtcaacgt gcagacgacc agcggtatcc accagcagca cgtcgtagaa tttcagtttg
     5461 gcttctttca gcgccgcgtt aacgatatct accggcttct gaccaacatc agaagggaag
     5521 aaatcaacgc ccacctgctc tgccagcgtc tcaagctgtt tgattgccgc cgggcgataa
     5581 acgtcggcag aaaccaccag cactttcttc ttgtgcttct cgcgcaggaa cttaccgagt
     5641 ttaccaacgc tggttgtttt accggcacct tgcaggcccg ccatcagtac gaccgcaggc
     5701 ggttgcgcag ccaggttcag ggtctggttc tcttcgccca tcgccgcaac cagttcgtta
     5761 cggactattt tgacgaactc ctgccccggc gtcaggctct tattaacttc atgaccaacc
     5821 gctttctctt ttacgcgatt gataaactca cgcactaccg gcagagcgac gtccgcctcc
     5881 agcagcgcca tgcgcacttc gcgcagcgta tcttttacgt tgtcttcagt gaggcgtcca
     5941 cggccactga tattgcgcag cgtgcgcgac aaacgatcgg ttaaattatc aaacattgtc
     6001 tctcgcctgg ggtggaaacg gttggccgca atcgcgacac atcatcagta ttttgccgca
     6061 gtataacatg aaggcgtctt tgttgttatg caacggttgg agcagcgttc acctgacgct
     6121 atactgcttc tctttcttat tgctcaaact gtcgacatta ctatgcccgt ttttgctctg
     6181 ctcgcgcttg tcgcctactc cgtcagtctt gcgctgattg ttcccggtct gctgcaaaaa
     6241 aacggcggct ggcggcgcat ggctattatt tctgcggtca ttgcgctggt ctgccacgca
     6301 atcgctctgg aagcccgcat cctgcccgac ggtgatagcg gacaaaacct cagcctgctg
     6361 aacgttggtt cattggtcag tttgatgatc tgtacggtaa tgaccattgt ggcttctcgc
     6421 aatcgtggct ggttgctgct acccattgtc tatgcctttg cgcttatcaa cctggcgctg
     6481 gcgaccttca tgcccaatga atacatcacc catctggaag caacgcctgg gatgctggtg
     6541 cacattggct tatcgctctt ttcctatgcc acgctgataa ttgccgccct gtacgcgcta
     6601 caactggcgt ggattgatta ccaactgaag aacaagaagc tggcgtttaa ccaagaaatg
     6661 ccgccattga tgagtatcga gcgtaaaatg ttccacatca cgcagattgg cgtggtgcta
     6721 ctaacgctca cgctttgcac tggcctgttc tacatgcaca acttatttag catggaaaat
     6781 atcgacaagg ccgtgctctc tatcgtggcg tggtttgtct atattgtgct gctgtgggga
     6841 cattatcatg aaggatggcg tggacgccgc gtcgtctggt ttaacgttgc gggtgcagtc
     6901 atcctgacac tggcctactt cggcagccga attgtccagc agttaatcag ctaaacccag
     6961 aaaaggagtt tcccctggaa cacatttcta ctactacgtt gatcattatt ctgatcatca
     7021 tggtggtcat ttcagcctat ttttccgggt ccgaaaccgg aatgatgacc ctcaaccgct
     7081 atcgtctgcg acatatggcg aaacagggta atcgctcggc caaacgcgtc gaaaaattgc
     7141 tgcgtaagcc agaccgcctg ataagcctgg tgttaatcgg caataacctg gtcaatattc
     7201 ttgcctccgc gctcggcact attgttggga tgcgtttgta cggcgatgcg ggcgtggcaa
     7261 ttgcgactgg tgtgctgact tttgtcgtac tggtttttgc tgaggtattg ccgaaaacca
     7321 ttgccgcgct gtacccggaa aaagtcgctt atccgagtag ttttctgctg gctccgctgc
     7381 aaattttgat gatgccgctg gtctggttgc tgaatgctat cacccgtatg ctgatgcgca
     7441 tgatgggtat caaaaccgat atcgtggtta gcggctcttt gagcaaagaa gagttgcgca
     7501 ctatcgtgca cgaatcacgc tcacaaattt cccgtcgcaa tcaggatatg ctactgtctg
     7561 tgctcgatct ggaaaagatg accgttgatg acatcatggt gccgcgcagt gaaattatcg
     7621 gtattgatat caacgatgac tggaaatcga ttctgcgcca actctcccac tcacctcacg
     7681 ggcgcatcgt gctctaccgt gattcgctgg acgacgccat cagtatgctg cgagtacgtg
     7741 aagcctggcg gttgatgtcg gagaaaaaag agttcaccaa agaaaccatg ctgcgcgccg
     7801 cggacgagat ctattttgtg ccggaaggta cgccgctcag cacgcagttg gtaaagtttc
     7861 agcgcaacaa aaagaaagtc ggcctggtcg tcaacgagta tggagacatt caggggctgg
     7921 tgacggttga agatattctg gaagagattg tcggcgattt cactacgtcg atgtcgccaa
     7981 cacttgccga agaggtcacg ccgcaaaacg acggttcggt gattatcgat ggcaccgcca
     8041 acgtgcggga aatcaacaaa gcctttaact ggcatctacc ggaagatgat gcccgcacgg
     8101 ttaatggcgt cattcttgag gcactggaag agatccctgt cgcaggcacc cgcgtgcgta
     8161 ttggcgagta cgatatagat attctcgacg tacaggacaa tatgattaag caggtaaaag
     8221 tttttcctgt gaaaccgctg cgcgagagtg tggcggagta acgaaaacgg ccctgcattc
     8281 gcatgcaggg ccgtgaatta ttacgaaaac agaaattacg cttttgcttt cgctacagtt
     8341 accatcgccg cacgaatcgt acgaccattc agcgtataac ccttctgcat aatgcccagt
     8401 acgttacctg gcgcaacgtc atcagattcc accattgcga tagcctgatg cacattcggg
     8461 tccagcggca cgttagtttc ggcgatcact tcaacaccaa acttacgcac aacatccagc
     8521 atcgacttca gcgtcagctc aatgccttca accatcgcag acatatccgg gttagcttta
     8581 tcagccactt ccagcgcacg atccaggcta tcaatcaccg gcagcaattc gttgatgaat
     8641 ttctccagcg cgaatttgtg ggctttttca atatccagtt cagtacgtcg acgcaggttt
     8701 tccatttcgg cttttacacg caaaatgccg tcacgttcac gggtctgggc ttcagccagc
     8761 tgagcttcga gattcgcaat tttttcatcg cgcggatcca cctgctcagc agaagcttct
     8821 ggctcaactg cctcaatctc ttcgtgctga tccatgataa tttcttccgg ggcttgcccc
     8881 tcaggcgttt tctgttcttt actactcatg aatttctccg cgtttttttc gcattcatct
     8941 cgctaacttc gcttattatg gggatcagtt tcagggtttc aagggaagca ctcacattgt
     9001 catcaatctt cgcaacaagg acctcggaaa aatgaataat catttcaagt gtattggcat
     9061 tgtgggacac ccacggcacc ccactgcact gacaacacat gaaatgctct accgctggct
     9121 gtgcacaaaa ggttacgagg tcatcgttga gcaacaaatc gctcacgaac tgcaactgaa
     9181 gaatgtgaaa actggcacgc tcgcggagat tgggcaacta gctgatctcg cggtagtcgt
     9241 tggtggcgac ggtaatatgc tgggcgcggc acgcacactc gcccgttacg atattaaagt
     9301 tattggaatc aaccgtggca acctgggttt cctgactgac cttgaccccg ataacgccca
     9361 gcaacagtta gccgatgtgc tggaaggcca ctacatcagc gagaaacgtt ttttgctgga
     9421 agcgcaagtc tgtcagcaag attgccagaa acgcatcagc accgcgataa atgaagtggt
     9481 gcttcatcca ggcaaagtgg cgcatatgat tgagttcgaa gtgtatatcg acgagatctt
     9541 tgcgttttct cagcgatctg atggactaat tatttcgacg ccaacaggct ccaccgccta
     9601 ttccctctct gcaggcggtc ctattctgac cccctctctg gatgcgatta ccctggtgcc
     9661 catgttcccg catacgttgt cagcacgacc actggtcata aacagcagca gcacgatccg
     9721 tctgcgtttt tcgcatcgcc gtaacgacct ggaaatcagt tgcgacagcc agatagcact
     9781 gccgattcag gaaggtgaag atgtcctgat tcgtcgctgt gattaccatc tgaatctgat
     9841 tcatccgaaa gattacagtt atttcaacac attaagcacc aagctcggct ggtcaaaaaa
     9901 attattctaa ttttacgcca gcctctttac tgtatataaa accagtttat actgtacaca
     9961 ataacagtaa tggtttttca tacaggaaaa cgactatgtt ggcacaactg accatcagca
    10021 actttgctat cgttcgtgag cttgagattg attttcatag cggcatgacc gtaataactg
    10081 gcgagaccgg cgcgggtaaa tctattgcaa tagatgccct cggtctttgt ctcggtggtc
    10141 gcgctgaagc cgacatggtg cgtaccggcg ctgctcgcgc tgacctgtgc gcccgttttt
    10201 ctctgaaaga tacgccagcg gctctgcgct ggctggaaga aaatcagctt gaagacgggc
    10261 atgaatgttt gcttcgtcga gtgatcagca gcgatggtcg ctcccgtggt ttcatcaacg
    10321 gtacagctgt tcctctgtca caactgcgcg aactgggtca gttgctgatt cagatccatg
    10381 gtcagcacgc tcatcaatta ctcaccaaac ctgagcacca aaaattcctg cttgatggct
    10441 atgccaatga aacctctcta ctgcaggaaa tgaccgcacg ttatcagttg tggcatcaaa
    10501 gctgccgtga cctcgcgcat catcaacagt taagtcagga acgcgccgcc cgtgcggaac
    10561 tgctgcaata ccaattaaaa gaacttaacg aatttaatcc gcagcccgga gagtttgaac
    10621 aaatcgacga agagtacaaa cgtctggcga acagcggtca attgctgacc accagccaga
    10681 atgcattggc attaatggcc gacggtgaag acgcaaacct gcaaagtcag ctttacacgg
    10741 ctaaacaact ggtgagcgaa ttgattggca tggacagcaa actgtccggc gtacttgata
    10801 tgctggaaga agctaccatc cagattgctg aagccagcga tgaactgcgc cactactgcg
    10861 atcgtctgga tctcgatccc aaccgactat ttgaacttga acagcgcatc tcaaaacaga
    10921 tttcgctggc acgtaaacat cacgtcagcc ctgaggcatt gccacagtat taccagtcgc
    10981 tactggaaga acagcagcaa ctggacgatc aggccgactc acaagaaacg cttgcgctgg
    11041 cggtaacgaa acatcatcag caggcactgg aaatcgcgcg cgcattacac caacaacgcc
    11101 agcaatatgc agaagaactt gcacagctga tcaccgacag tatgcatgcg ctctcaatgc
    11161 cgcatgggca gtttacgatc gatgttaaat ttgacgagca tcacctgggc gctgacggtg
    11221 ccgatcgtat tgagtttcgg gtaaccacca acccaggtca gccaatgcag cctattgcca
    11281 aagtcgcatc cggtggtgaa ttgtcccgca tcgcactggc aatccaggtc atcacggcgc
    11341 gtaaaatgga aaccccggca ctgatttttg atgaagtgga tgtagggatt agcggtccaa
    11401 cagcggcagt tgtcggcaaa ctgctgcgtc aactcggcga atcaactcag gtgatgtgtg
    11461 ttacccacct gccacaagtc gcgggatgtg gtcatcaaca ctattttgtc agcaaagaaa
    11521 ccgatggtgc gatgacagaa acgcatatgc aatccctgaa taaaaaagcg cggttacaag
    11581 agctggcgcg cctgcttggt ggcagtgaag tcacacgtaa tacactggcg aatgcgaaag
    11641 aactgcttgc agcgtaaact tttttcctgc ttcacggtca gagtaaacag caaaacgccg
    11701 taagaccgga aagcaaaagg ttttaaagtg atgaaaggtc tattatcatc ggcatattac
    11761 agatgagcca cgtactgctc gggcccgaaa aggaatcaaa tcactatgcg ctgtaaaacg
    11821 ctgactgctg cagcagcagt actattgatg ttgaccgcag gctgttccac tctggagcga
    11881 gtggtttacc gtcctgacat caaccagggg aactatctga ccgctaacga cgtatccaaa
    11941 atacgtgttg gcatgacgca acaacaagtt gcgtacgcat tgggtacacc gctgatgtcc
    12001 gatccatttg gtacgaatac ctggttctat gtcttccgcc agcaaccagg tcatgaaggt
    12061 gtaacgcagc aaacgctgac gctgaccttt aacagtagcg gtgtgttgac caatattgat
    12121 aacaaacctg cgctgagtgg taactaataa taaagttgct ctcaaagacg ttaaaaaagg
    12181 tgctcaatga gcaccttttt tctgtctgtt atttattcgc tgatttttct gctcgttgcc
    12241 tgcgaagctc tttcggatcg gcaatgagag ggcgataaat ctccacccga tcgccatcat
    12301 gcacactatc gcttagtttt gccggacggc tgtaaatgcc gactttgttt ttagttaaat
    12361 cgatatcggt acgcaattcc agcaagccac tggcgcgaat agcttcttca accgtcgcgc
    12421 cctcctgcag cgtcacgcgc tgcaggtact gcttctcagg tagcgcataa gccacctcaa
    12481 cggcaatttt acctggcact gtaaacctct ttcgcacgaa ccgtaaaagc ctggaccata
    12541 ttagacgcca gctctttaaa cacgcgacca aaggcgagtt caatcaactt attggtaaac
    12601 tcaaagtcga gatgaaactc gatacgacac gcctcctggc tcagcggcgt aaacttccat
    12661 ccaccaatca atttcttgaa cggcccatcc accagattca taagaatact ttggttactg
    12721 gtcaactggt tgcgggtagt aaacgttttg ctgatcccag ccttagagac atctaccgcc
    12781 gcagtcatct gcccaggagt ggactccaga atccgacttc cggtacaacc cggcaaaaac
    12841 tgaggataag actgaacgtc attcactaac tgatacattt gctccgcgct gtagggtacc
    12901 agtgcggtcc ggctaatctg aggcataaca atttccatca acaaaaatcc aacaaataat
    12961 atcatttatc ctgctgtaaa aaaaacgcta tcccggcgct gggtaacatc gggttcatgc
    13021 taagatagag ccttgtcccc cgcaggattg atatggggtg ttttcgattt cagattaccg
    13081 atgattcacg acgcttatga cgaagaaaaa agcacataaa cctggttcag cgaccatcgc
    13141 gcttaacaag cgcgcccgtc acgaatactt tatcgaagaa gagttcgaag cgggacttgc
    13201 cctgcaaggc tgggaggtta aatccctgcg cgcaggaaaa gccaatatca gcgacagcta
    13261 cgtccttctg cgtgacggag aggcatttct gtttggcgct aacatcacgc caatggccgt
    13321 ggcctccacg catgtggtgt gcgatcctac ccgtacccgc aagttacttc tcaaccagcg
    13381 cgaactggac tcattgtacg gtcgcgtcaa tcgagaaggc tataccgtag tggcgctctc
    13441 cctgtactgg aaaaatgcct ggtgcaaagt gaaaatcggc gtcgccaaag gtaaaaaaca
    13501 gcacgataaa cgttcagata tcaaagaacg cgaatggcag gtggataaag cacgtatcat
    13561 gaaaaacgcc caccgttaaa cctgcactcc aattattgac cagttcctca ccgcgcctcc
    13621 ctctccggcg gcgcgaatga acatcttatt ggctatcaca tccgacacaa atgttgccat
    13681 cccattgctt aatcgaataa aaatcaggct acatgggtgc taaatcttta acgataacgc
    13741 cattgaggct ggtcatggcg ctcataaatc tggtatactt acctttacac attggggctg
    13801 attctggatt cgacgggatt tgcgaaaccc aaggtgcatg ccgaggggcg gttggcctcg
    13861 taaaaagccg caaaaaatag tcgcaaacga cgaaaactac gctttagcag cttaataacc
    13921 tgcttagagc cctctctccc tagcctccgc tcttaggacg gggatcaaga gaggtcaaac
    13981 ccaaaagaga tcgcgtggaa gccctgcctg gggttgaagc gttaaaactt aatcaggcta
    14041 gtttgttagt ggcgtgtccg tccgcagctg gcaagcgaat gtaaagactg actaagcatg
    14101 tagtaccgag gatgtaggaa tttcggacgc gggttcaact cccgccagct ccaccacttt
    14161 tgataggact gcaatcggac agcggcaata aaaacagcca cttacggact ctgaccagac
    14221 agagcgcaga ccgagaaaag acaaaaaaat gcacgtaaaa tgcacgtgca ctttaaaaga
    14281 accccagatc tcacggtctg gtttttttct gtttgtaact aatggtaaca aaaacacatc
    14341 acctcttggc gttccttctc cttgatactg tttatccata cagttaaaaa taacactgta
    14401 tacaaacaca gtatagagag atttttatgc gtattgaaat ttgcatagcc aaagaaaaaa
    14461 tcagcaaaat gccaaaagga tctgttcctg ccctacagca agaaatgctg tgacgtgcca
    14521 gtaaacgtta tgacgatgta gaagtgatca tcaaatccac cagcaacgat ggcctttcag
    14581 ttactctcac cgccgataaa gattctgcaa aaacttttgt tcaggagacg ctgaaagata
    14641 cctgggagtc tgctgacgag tggtttgttc gctaattaac gagtaaaatc agtaacggct
    14701 ggaaatcatt caatactcac actatctaaa agttcaccag ccaacagcgg taagttcttt
    14761 catacgacgt gccgcggatc ctattattct agtatgaaat ccctaaccga tttcaaattc
    14821 tccagactgc tcaga
//
LOCUS       DAZKHF020000105        14834 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2201S31241
            SAMN43267800-rid25106413.denovo.106, whole genome shotgun sequence.
ACCESSION   DAZKHF020000105 DAZKHF020000000
VERSION     DAZKHF020000105.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267800
            Sequence Read Archive: SRR30307263
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 14834)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14834)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 14834)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350581.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350581.2
            Genome Coverage       :: 151x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:21:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,652
            CDSs (total)                      :: 5,557
            Genes (coding)                    :: 5,320
            CDSs (with protein)               :: 5,320
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 237
            CDSs (without protein)            :: 237
            Pseudo Genes (ambiguous residues) :: 0 of 237
            Pseudo Genes (frameshifted)       :: 102 of 237
            Pseudo Genes (incomplete)         :: 130 of 237
            Pseudo Genes (internal stop)      :: 52 of 237
            Pseudo Genes (multiple problems)  :: 41 of 237
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14834
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267800-rid25106413.denovo.106"
                     /serotype="O 26:H11"
                     /isolate="2201S31241"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-01"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            24..365
                     /locus_tag="ACHOSC_RS12985"
                     /old_locus_tag="pgaptmp_002597"
                     /pseudo
     CDS             24..365
                     /locus_tag="ACHOSC_RS12985"
                     /old_locus_tag="pgaptmp_002597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308469.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2773 domain-containing protein"
     gene            362..445
                     /locus_tag="ACHOSC_RS12990"
                     /old_locus_tag="pgaptmp_002598"
     CDS             362..445
                     /locus_tag="ACHOSC_RS12990"
                     /old_locus_tag="pgaptmp_002598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4548518.1"
                     /translation="MTQRPWSKLQHKTHNIAALKIIARRSE"
     gene            complement(523..1434)
                     /gene="rdgC"
                     /locus_tag="ACHOSC_RS12995"
                     /old_locus_tag="pgaptmp_002599"
     CDS             complement(523..1434)
                     /gene="rdgC"
                     /locus_tag="ACHOSC_RS12995"
                     /old_locus_tag="pgaptmp_002599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005120935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombination-associated protein RdgC"
                     /protein_id="HGU4548519.1"
                     /translation="MLWFKNLMVYRLSREISLRAEEMEKQLASMAFTPCGSQDMAKMG
                     WVPPMGSHSDALTHVANGQIVICARKEEKILPSPVIKQALEAKIAKLEAEQARKLKKT
                     EKDSLKDEVLHSLLPRAFSRFSQTMMWIDTVNGLIMVDCASAKKAEDTLALLRKSLGS
                     LPVVPLSMENPIELTLTEWVRSGSAAQGFQLLDEAELKSLLEDGGVIRAKKQDLTSEE
                     ITNHIEAGKVVTKLALDWQQRIQFVMCDDGSLKRLKFCDELRDQNEDIDREDFAQRFD
                     ADFILMTGELAALIQNLIEGLGGEAQR"
     gene            1559..2467
                     /gene="mak"
                     /locus_tag="ACHOSC_RS13000"
                     /old_locus_tag="pgaptmp_002600"
     CDS             1559..2467
                     /gene="mak"
                     /locus_tag="ACHOSC_RS13000"
                     /old_locus_tag="pgaptmp_002600"
                     /EC_number="2.7.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308471.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructokinase"
                     /protein_id="HGU4548520.1"
                     /translation="MRIGIDLGGTKTEVIALGDAGEQLYRHRLPTPRDDYRQTIETIA
                     TLVDMAEQATGQRGTVGMGIPGSISPYTGVVKNANSTWLNGQPFDKDLSARLQREVRL
                     ANDANCLAVSEAVDGAAAGAQTVFAVIIGTGCGAGVAFNGRAHIGGNGTAGEWGHNPL
                     PWMDEDELRYREEVPCYCGKQGCIETFISGTGFATDYRRLSGHALKGSEIISLVEESD
                     PVAELALRRYELRLAKSLAHVVNILDPDVIVLGGGMSNVDRLYQTVPQLIKQFVFGGE
                     CETPVRKAKHGDSSGVRGAAWLWPQE"
     gene            complement(2610..3794)
                     /gene="araJ"
                     /locus_tag="ACHOSC_RS13005"
                     /old_locus_tag="pgaptmp_002601"
     CDS             complement(2610..3794)
                     /gene="araJ"
                     /locus_tag="ACHOSC_RS13005"
                     /old_locus_tag="pgaptmp_002601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414930.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter AraJ"
                     /protein_id="HGU4548521.1"
                     /translation="MKKVILSLALGTFGLGMAEFGIMGVLTELAHNVGISIPAAGHMI
                     SYYALGVVVGAPIIALFSSRYSLKHILLFLVALCVIGNAMFTLSSSYLMLAIGRLVSG
                     FPHGAFFGVGAIVLSKIIKPGKVTAAVAGMVSGMTVANLLGIPLGTYLSQEFSWRYTF
                     LLIAVFNIAVMASVYFWVPDIRDEAKGKLREQFHFLRSPAPWLIFAATMFGNAGVFAW
                     FSYVKPYMMFISGFSETAMTFIMMLVGLGMVLGNMLSGRISGRYSPLRIAAVTDFIIV
                     LALLMLFFCCGMKTTSLIFAFICCAGLFALSAPLQILLLQNAKGGELLGAAGGQIAFN
                     LGSAVGAYCGGMMLTLGLAYNYVALPAALLSFAAMSSLLLYGRYKRQQAADSPVLAKP
                     LG"
     gene            complement(3920..7063)
                     /gene="sbcC"
                     /locus_tag="ACHOSC_RS13010"
                     /old_locus_tag="pgaptmp_002602"
     CDS             complement(3920..7063)
                     /gene="sbcC"
                     /locus_tag="ACHOSC_RS13010"
                     /old_locus_tag="pgaptmp_002602"
                     /EC_number="3.1.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004527 - exonuclease activity [Evidence
                     IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease subunit SbcC"
                     /protein_id="HGU4548522.1"
                     /translation="MKILSLRLKNLNSLKGEWKIDFTREPFASNGLFAITGPTGAGKT
                     TLLDAICLALYHETPRLSNVSQSQNDLMTRDTAECLAEVEFEVKGEAYRAFWSQNRAR
                     NQPDGNLQVPRVELARCADGKILADKVKDKLELTATLTGLDYGRFTRSMLLSQGQFAA
                     FLNAKPKERAELLEELTGTEIYGQISAMVFEQHKSARTELEKLQAQASGVALLTPEQV
                     QSLTASLQVLTDEEKQLLTAQQQEQQSLNWLTRQDELQQEASRRQQALQQVLAEEEKA
                     QPQLAALSLAQPARNLRPHWERIAEHSAALAHIRQRIEEVNTRLQSTMALRASIRHHA
                     AKQSAELQQQQQSLNTWLQEHDRFRQWNNELAGWRAQFSQQTSDREHLRQWQQQLTHA
                     EQKLNALAAITLTLTADEVATALAQHAEQRPLRQRLVALHGQIVPQQKRLAQLMVTIQ
                     NVTLEQTQRNVALNEMRQRYKEKTQQLADVKTICEQEARIKTLEAQRAQLQAGQPCPL
                     CGSTSHPAVEAYQALEPGVNQSRLLALENEVKKLGEEGAALRGQLDALTKQLQRDENE
                     AQSLRQDEQALTQQWQAVTASLNITLQPQDDIQPWLDAQDEHERQLRLLSQRHELQGQ
                     IAAHNQQIIQYQQQIEQRQQQLLTALAGYALTLPQEDEEESWLATRQQEAQSWQQRQN
                     ELTALQNRIQQLTPILETLPQSDDLPHSEETVALDNWRQVHEQCLALHSQQQTLQQQD
                     VLAAQSLQKAQAQFDTALQASVFDDQQAFLAALMDEQTLTQLEQLKQNLENQRRQAQT
                     LVTQTAETLAQHQQHRPDGLALTVTVEQIQQELAQTHQKLRENTTSQGEIRQQLKQDA
                     DNRQQQQTLMQQIAQMTQQVEDWGYLNSLIGSKEGDKFRKFAQGLTLDNLVHLANQQL
                     TRLHGRYLLQRKASETLEVEVVDTWQADAVRDTRTLSGGESFLVSLALALALSDLVSH
                     KTRIDSLFLDEGFGTLDSETLDTALDALDALNASGKTIGVISHVEAMKERIPVQIKVK
                     KINGLGYSKLESTFAVK"
     gene            complement(7060..8262)
                     /gene="sbcD"
                     /locus_tag="ACHOSC_RS13015"
                     /old_locus_tag="pgaptmp_002603"
     CDS             complement(7060..8262)
                     /gene="sbcD"
                     /locus_tag="ACHOSC_RS13015"
                     /old_locus_tag="pgaptmp_002603"
                     /EC_number="3.1.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004527 - exonuclease activity [Evidence
                     IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease subunit SbcD"
                     /protein_id="HGU4548523.1"
                     /translation="MRILHTSDWHLGQNFYSKSREAEHQAFLDWLLETAQTHQVDAII
                     VAGDVFDTGSPPSYARTLYNRFVVNLQQTGCHLVVLAGNHDSVATLNESRDIMAFLNT
                     TVVASAGHAPQILPRRDGTPGAVLCPIPFLRPRDIITSQAGLNGIEKQQHLLAAITDY
                     YQQHYADACKLRGDQPLPIIATGHLTTVGASKSDAVRDIYIGTLDAFPAQNFPPADYI
                     ALGHIHRAQIIGGMEHVRYCGSPIPLSFDECGKSKYVHLVTFSNGKLESVENLNVPVT
                     QPMAVLKGDLASITAQLEQWRDVSQEPPVWLDIEITTDEYLHDIQRKIQALTESLPVE
                     VLLVRRSREQRERVLASQQRETLSELSVEEVFNRRLALEELDESQQQRLQHLFTTTLH
                     TLAGEHEA"
     gene            8452..9141
                     /gene="phoB"
                     /locus_tag="ACHOSC_RS13020"
                     /old_locus_tag="pgaptmp_002604"
     CDS             8452..9141
                     /gene="phoB"
                     /locus_tag="ACHOSC_RS13020"
                     /old_locus_tag="pgaptmp_002604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000113934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate response regulator transcription
                     factor PhoB"
                     /protein_id="HGU4548524.1"
                     /translation="MTRRILVVEDEAPIREMVCFVLEQNGFQPVEAEDYDSAVNQLNE
                     PWPDLILLDWMLPGGSGIQFIKHLKRESMTRDIPVVMLTARGEEEDRVRGLETGADDY
                     ITKPFSPKELVARIKAVMRRISPMAVEEVIEMQGLSLDPTSHRVMAGEEPLEMGPTEF
                     KLLHFFMTHPERVYSREQLLNHVWGTNVYVEDRTVDVHIRRLRKALEPGGHDRMVQTV
                     RGTGYRFSTRF"
     gene            9199..10494
                     /gene="phoR"
                     /locus_tag="ACHOSC_RS13025"
                     /old_locus_tag="pgaptmp_002605"
     CDS             9199..10494
                     /gene="phoR"
                     /locus_tag="ACHOSC_RS13025"
                     /old_locus_tag="pgaptmp_002605"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004721 - phosphoprotein phosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate regulon sensor histidine kinase PhoR"
                     /protein_id="HGU4548525.1"
                     /translation="MLERLSWKRLVLELLLCCLPAFILGAFFGYLPWFLLASVTGLLI
                     WHFWNLLRLSWWLWVDRSMTPPPGRGSWEPLLYGLHQMQLRNKKRRRELGNLIKRFRS
                     GAESLPDAVVLTTEEGGIFWCNGLAQQILGLRWPEDNGQNILNLLRYPEFTQYLKTRD
                     FSRPLNLVLNTGRHLEIRVMPYTHKQLLMVARDVTQMHQLEGARRNFFANVSHELRTP
                     LTVLQGYLEMMDEQPLEGAVREKALHTMREQTQRMEGLVKQLLTLSKIEAAPTQLLNE
                     KVDVPMMLRVVEREAQTLSQKKQTFTFEIDNGLKVSGNEDQLRSAISNLVYNAVNHTP
                     EGTHITVRWLRVPHGAEFSVEDNGPGIAPEHIPRLTERFYRVDKARSRQTGGSGLGLA
                     IVKHAVNHHESRLNIESTVGKGTRFSFVIPERLIAKNSD"
     gene            10901..12220
                     /gene="brnQ"
                     /locus_tag="ACHOSC_RS13030"
                     /old_locus_tag="pgaptmp_002606"
     CDS             10901..12220
                     /gene="brnQ"
                     /locus_tag="ACHOSC_RS13030"
                     /old_locus_tag="pgaptmp_002606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid transporter carrier
                     protein BrnQ"
                     /protein_id="HGU4548526.1"
                     /translation="MTHQLRSRDIIALGFMTFALFVGAGNIIFPPMVGLQAGEHVWTA
                     AFGFLITAVGLPVLTVVALAKVGGGVDSLSTPIGKVAGVLLATVCYLAVGPLFATPRT
                     ATVSFEVGIAPLTGDSALPLFIYSLVYFAIVILVSLYPGKLLDTVGNFLAPLKIIALV
                     ILSVAAIVWPAGSISTATEAYQNAAFSNGFVNGYLTMDTLGAMVFGIVIVNAARSRGV
                     TEARLLTRYTVWAGLMAGVGLTLLYLALFRLGSDSASLVDQSANGAAILHAYVQHTFG
                     GGGSFLLAALIFIACLVTAVGLTCACAEFFAQYVPLSYRTLVFILGGFSMVVSNLGLS
                     QLIQISVPVLTAIYPPCIALVVLSFTRSWWHNSSRVIAPPMFISLLFGILDGIKASAF
                     SDILPSWAQRLPLAEQGLAWLMPTVVMVVLAIIWDRAAGRQVTSSAH"
     gene            12296..13669
                     /gene="proY"
                     /locus_tag="ACHOSC_RS13035"
                     /old_locus_tag="pgaptmp_002607"
     CDS             12296..13669
                     /gene="proY"
                     /locus_tag="ACHOSC_RS13035"
                     /old_locus_tag="pgaptmp_002607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="proline-specific permease ProY"
                     /protein_id="HGU4548527.1"
                     /translation="MESKNKLKRGLSTRHIRFMALGSAIGTGLFYGSADAIKMAGPSV
                     LLAYIIGGIAAYIIMRALGEMSVHNPAASSFSRYAQENLGPLAGYITGWTYCFEILIV
                     AIADVTAFGIYMGVWFPTVPHWIWVLSVVLIICAVNLMSVKVFGELEFWFSFFKVATI
                     IIMIVAGFGIIIWGIGNGGQPTGIHNLWSNGGFFSNGWLGMVMSLQMVMFAYGGIEII
                     GITAGEAKDPEKSIPRAINSVPMRILVFYVGTLFVIMSIYPWNQVGTAGSPFVLTFQH
                     MGITFAASILNFVVLTASLSAINSDVFGVGRMLHGMAEQGSAPKIFSKTSRRGIPWVT
                     VLVMTTALLFAVYLNYIMPENVFLVIASLATFATVWVWIMILLSQIAFRRRLPPEEVK
                     ALKFKVPGGVATTIGGLIFLLFIIGLIGYHPDTRISLYVGFAWIVVLLIGWMFKRRHD
                     RQLAENQ"
     gene            13825..>14834
                     /gene="malZ"
                     /locus_tag="ACHOSC_RS13040"
                     /old_locus_tag="pgaptmp_002608"
     CDS             13825..>14834
                     /gene="malZ"
                     /locus_tag="ACHOSC_RS13040"
                     /old_locus_tag="pgaptmp_002608"
                     /EC_number="3.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308480.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004558 - alpha-1,4-glucosidase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="maltodextrin glucosidase"
                     /protein_id="HGU4548528.1"
                     /translation="MMLNAWHLPVPPFVKQSKDQLLITLWLTGEDPPQRIMLRTEHDN
                     EEMSVSMHKQRSQQQPGVTAWRAAIDLSSGQPRRRYSFKLLWHDRQRWFTPQGFSRMP
                     PARLEQFAVDVPDIGPQWAADQIFYQIFPDRFARSLPREAEQDHVYYHHAAGQEIILR
                     DWDEPVTAQAGGSTFYGGDLDGISEKLPYLKKLGVTALYLNPVFKAPSVHKYDTEDYR
                     HVDPQFDGDGALLRLRHNTQQLGMRLVLDGVFNHSGDSHAWFDRHNRGTGGACHNPES
                     PWRDWYSFSDDGTALDWLGYASLPKLDYQSESLVNEIYRGEDSIVRHWLKAPWSMDGW
                     RL"
BASE COUNT         3262 a         3794 c         4017 g         3761 t
ORIGIN      
        1 ataaatatca gtgaagatct ccagatattg ttgcggaact ggctacgata aaagataaat
       61 cagatgatga atggtggcgt gcattgctgc aaagtcgcaa tgatcatttg cgccaaacgg
      121 cattacgcaa cgcacatacc ccggcgtcgt tgttaacgac attgaccgag tctcggcatc
      181 ggtcgctagc tatgaataat ccacagctcg ctgccgatgt gaaaacgacg tggttaaaag
      241 aggatccatc attactctta tttgtcgaac aacccgatct ttcgctgtta cgggatttag
      301 tgaaaaccgg ggcaatgcgg aaaattcgca gtgaagcgcg tcactggctt gaggaaaaac
      361 aatgacacaa cgtccatgga gcaagttaca gcataaaacg cataatattg ctgcgctaaa
      421 aattattgcc cgccgtagcg aataattata tgcctggtgt ggcttcgtac gccggataag
      481 acgcggcagg cgtcgcatcc ggcattaaag gaaaatcagc aattaacgtt gtgcttcgcc
      541 acctaatcct tcaatcaggt tttgaattaa cgctgccagt tcaccagtca tcaggatgaa
      601 atcggcatca aaacgctggg cgaaatcttc acggtcgata tcttcgtttt gatcgcgcag
      661 ctcgtcgcag aacttcagac gcttgagcga accatcgtcg cacatcacaa actgaatgcg
      721 ctgctgccag tcgagcgcca gtttagtcac cacttttccg gcttcaatgt gattggtgat
      781 ctcttcgctg gtcagatctt gtttcttcgc gcggatcacg ccgccatctt ccagcaacga
      841 tttcagctcg gcttcatcaa gcagctggaa gccctgtgcc gcactaccgg agcgaaccca
      901 ttcggtcagc gtcagttcaa tcgggttttc catgctcaat ggtacaaccg gtaacgaccc
      961 caggctttta cgcagtaatg ccagcgtatc ttccgctttt ttggcactgg cgcagtccac
     1021 cataatcaaa ccgttaaccg tgtcgatcca catcattgtc tggctaaaac ggctgaaagc
     1081 acgcggcagc agagagtgca gcacttcgtc tttcagcgaa tctttttcgg ttttcttcag
     1141 cttacgcgcc tgttccgctt ccagtttggc gattttcgct tccagcgcct gtttaatcac
     1201 cggagacggg aggatttttt cttctttgcg cgcgcagata acaatttgac cattggcaac
     1261 gtgcgttaac gcatcgctgt gcgatcccat cggaggaacc cagcccatct tcgccatgtc
     1321 ctggctgccg catggggtaa atgccatcga ggctagctgt ttttccatct cttctgcacg
     1381 cagcgaaatc tcgcggctaa gacggtaaac cattaaattt ttgaaccaca gcatgataat
     1441 ttccacggcc ttgtcgttaa atttagcggg catgataacg aattgtcggc ggccttgcat
     1501 tgccaatccg gttgtccgtc tctacgctat tgatattgaa aaaaataagg agagtaccgt
     1561 gcgtataggt atcgatttag gcggcaccaa aactgaagtg attgcactgg gcgatgcagg
     1621 ggagcagttg taccgccatc gtctgcccac gccgcgtgat gattaccggc aaactattga
     1681 aacgatcgcc acgttggttg atatggcgga gcaggcgaca gggcagcgcg gaacggtagg
     1741 tatgggcatt ccaggttcga tttcgcccta caccggcgtg gtgaagaatg ccaattcaac
     1801 ctggctcaac ggtcagccat tcgataaaga cttaagcgcg cggttgcagc gggaagtgcg
     1861 gctggcaaat gacgccaact gtctggcggt ttcagaagcg gtagatggcg cggcagcggg
     1921 agcgcagacg gtatttgccg tgattatcgg cacgggatgc ggcgcgggcg tggcattcaa
     1981 tgggcgggcg catatcggcg gcaatggcac ggcaggtgag tggggacaca atccgctacc
     2041 gtggatggac gaagacgaac tgcgttatcg cgaggaagtc ccttgttatt gcggtaaaca
     2101 aggttgtatt gaaaccttta tttcgggcac tggattcgcg acggattatc gtcgtttgag
     2161 cggacatgcg ctgaaaggca gtgaaattat cagcctggtt gaagaaagcg atccggtagc
     2221 ggaactggca ttgcgtcgct acgagctgcg gctggcaaaa tcgctggcac atgtcgtgaa
     2281 tattctcgat ccggatgtga ttgtcctggg gggcgggatg agcaatgtag accgtttata
     2341 tcaaacggtt ccgcagttga ttaaacaatt tgtcttcggc ggcgaatgtg aaacgccggt
     2401 gcgtaaggcg aagcacggtg attccagcgg cgtacgcggc gctgcgtggt tatggccaca
     2461 agagtaaaaa acgtaggcaa ttggcgcatc atgcctgatg cgacgcttgc cgcgtcttat
     2521 caggcctaca aaatgtgcca gaaccgcgta gggcggataa ggcgttcacg ccgcatccgg
     2581 caataagcaa cgcgcaccgt gacagtaatc taccccagtg gtttcgccag caccggacta
     2641 tccgccgctt gctggcgctt atagcgacca tacagcagca acgatgacat cgcagcaaac
     2701 gaaagcaggg cggcaggcag cgccacgtaa ttatatgcca gccccagcgt cagcatcata
     2761 cctccgcaat atgcgccgac ggcgctaccg aggttaaacg ctatttgccc acctgcggca
     2821 cctaataact ctccgccttt ggcgttttgt agtaacaata tttgcagagg tgctgaaagg
     2881 gcaaataatc ccgcgcaaca aataaaagca aaaataagcg acgttgtttt catgccgcag
     2941 cagaaaaaga gcatcagcag tgccagtaca attataaagt cagtcactgc tgcaatgcgc
     3001 agtggtgaat aacgtcctga aatcctgcca cttagcatat ttcccaacac catccctagc
     3061 ccaactaaca tcataataaa ggtcatcgcc gtttccgaaa aaccggaaat aaacatcatg
     3121 tatggcttta cgtagctgaa ccaggcaaac acacctgcgt tgccaaacat cgtggcggcg
     3181 aaaattaacc acggggccgg gctgcgtaaa aagtgaaatt gttcgcgcag ctttcctttc
     3241 gcctcgtcgc gaatatccgg cacccaaaaa tagaccgatg ccatcaccgc aatattaaaa
     3301 acagcgatca ataaaaaggt gtaacgccag ctaaattcct gacttaaata cgttcccagc
     3361 ggaatgccca gcaaattggc gactgtcatc ccggaaacca tccccgccac ggcggcggtg
     3421 acttttccgg gtttgataat ttttgataac acgatcgcgc cgacgccaaa aaatgcgcca
     3481 tgcggaaagc cggaaaccag gcgaccaatg gcaagcatca ggtaagacga agagagcgtg
     3541 aacatggcgt tgccaatgac gcacaacgcc accagaaaca acaagatatg tttgagtgag
     3601 tagcggctgg aaaagagtgc gatgattggc gcaccgacca ccactcccag cgcataatac
     3661 gagatcatat gcccggcagc aggaatcgaa attcctacgt tatgcgccag ctccgtgagc
     3721 acgcccataa tgccaaattc ggccatcccc aaaccaaacg tgcccagagc caaagataaa
     3781 atgacttttt tcataccacc atcacaacta ccagaacggc taccaatcac cgtagccaat
     3841 aaagtgatag cttgcaggat aacgaccagc aaggccaaga gataattcgc ccctctgtat
     3901 tcattatcct gctgaatagt tatttcactg caaacgtact ttccagtttg ctgtagccta
     3961 ggccgttgat ctttttcact ttgatctgca ccggaatacg ctctttcatc gcttctacgt
     4021 ggctaatcac accgatggtt ttgccactgg cgttcagggc atccagcgca tcaagggcgg
     4081 tatccagcgt ttcgctatcc agcgtgccaa aaccttcatc aaggaacagc gagtcaatac
     4141 gtgttttatg gctgaccaga tccgaaagcg ccagcgccag cgccagacta acgaggaaac
     4201 tttcgccgcc ggaaagggta cgggtatcgc gtaccgcatc tgcctgccag gtatcaacaa
     4261 cctcgacttc cagcgtctcg ctggctttgc gctgtaacag atagcgcccg tgcagccggg
     4321 taagttgctg attagcgaga tggactaaat tatccagcgt cagcccctgg gcaaacttgc
     4381 ggaatttatc gccctctttg gaacctatta gcgaattcag atatccccag tcctcaacct
     4441 gctgcgtcat ttgagcaatt tgctgcatta aggtttgttg ttgctgacgg ttatctgcat
     4501 cctgcttcag ctgctggcga atctcgcctt gactcgtcgt gttttcacgc aacttttggt
     4561 gagtttgcgc taactcttgc tgaatctgct ccaccgtcac agtgagagcc aacccgtcag
     4621 gtcggtgttg ttgatgctgt gccagcgttt ctgctgtctg agtgaccagc gtttgcgcct
     4681 gacggcgctg gttttccaga ttctgcttga gctgttccag ctgcgttagt gtttgttcat
     4741 ccattagcgc cgcaaggaac gcctgctgat cgtcaaagac gctggcctgt agcgcggtgt
     4801 caaactgcgc ctgggctttt tgcagacttt gcgccgccag aacatcctgt tgctgtaatg
     4861 tctgctgctg gctgtgtaat gcgagacatt gttcatgaac ctgccgccag ttatccagcg
     4921 ccacagtttc ttcgctgtgc gggagatcat cactttgcgg caacgtttcc agaatcggcg
     4981 tcagctgctg aatacggttt tgcagcgcgg ttaattcgtt ctggcgttgc tgccagctct
     5041 gcgcttcttg ctgacgtgtc gccaaccagc tctcttcttc atcttcctgt ggcaatgtca
     5101 gtgcataacc cgccaatgcc gttaagagct gctgctggcg ttgttcaatt tgctgttgat
     5161 actggataat ttgctgatta tgcgcggcaa tctgcccttg taattcatgt cgttggctga
     5221 gtaaccgcag ctggcgttcg tgctcatctt gtgcatcaag ccacggttga atatcgtcct
     5281 gtggctgcaa ggtgatattg aggctggccg tgacggcttg ccattgttga gtaagtgctt
     5341 gctcatcttg tcggaggctt tgcgcttcgt tttcatcgcg ctgaagctgc ttcgttaatg
     5401 catccagttg cccgcgcagc gccgcacctt cttcgccgag ctttttaact tcgttttcca
     5461 gcgccagtaa tcgagactga ttaacgccag gctccagcgc ctgatacgcc tcgaccgccg
     5521 gatggctggt ggaaccacaa agtggacaag gctgacccgc ctgtaactgt gcacgctggg
     5581 cttccagcgt tttgatgcgc gcttcctgct cgcaaatggt tttcacatcg gcaagttgct
     5641 gcgtcttttc tttataacgc tggcgcattt cgttaagtgc gacattacgt tgcgtctgtt
     5701 ccagagtgac attctggata gtgaccatta actgcgccag acgtttttgt tggggaacaa
     5761 tctgcccatg cagcgcgacc aggcgctgac gcagtgggcg ttgctcagca tgttgcgcca
     5821 gggcggtagc aacttcatcg gcggttaacg tcaacgtgat cgccgcaagc gcattaagtt
     5881 tttgctcagc atgggttaac tgttgctgcc attgccgcag atgctcgcga tcgctggttt
     5941 gttgggagaa ctgcgcacgc caacccgcca gttcgttgtt ccactgacgg aagcggtcgt
     6001 gttcctgtaa ccaggtattc aggctttgtt gctgctgctg taattctgct gactgcttcg
     6061 ccgcgtggtg gcgaatgctc gcgcgaagcg ccattgtgct ctgtaagcga gtatttactt
     6121 cttcaatccg ctggcgaata tgcgccagcg ccgcgctgtg ttctgcgatg cgttcccagt
     6181 gtggacgaag atttcgtgcc ggttgtgcca gactaagcgc cgccagttga ggttgcgctt
     6241 tttcttcttc ggctaacacc tgttgcaagg cctgctgacg gcggctggct tcttgctgca
     6301 attcgtcctg acgcgttaac cagtttagcg attgttgttc ttgctgctgc gcggtaagta
     6361 actgtttttc ttcgtcagta agtacctgca aactcgctgt cagcgattgc acttgttccg
     6421 gcgtgagcaa cgcgacgccg ctggcctgcg cttgcagctt ctccagctct gtgcgggccg
     6481 atttgtgctg ctcaaaaacc atcgccgaga tttgcccgta gatttcagtg ccggttaact
     6541 cctcgagcaa ttccgcgcgt tctttgggtt tggcattcag gaaggcagca aattgcccct
     6601 gcgaaagcag catcgaacgg gtgaagcgcc cgtaatccag cccggttaac gtcgctgtca
     6661 gttccagctt atctttcact ttgtcggcga gaattttgcc gtcggcgcag cgcgccagct
     6721 ctacgcgtgg cacctgcaaa ttaccgtcgg gttggttacg cgcccgattc tggctccaga
     6781 atgcacggta cgcttcacct ttcacttcaa actccacctc cgccagacat tcggcggtat
     6841 cgcgggtcat gagatcattt tgcgattgtg aaacgttaga gagacgcgga gtttcatgat
     6901 acagcgccag acaaatggcg tccagcaggg tggttttccc cgcacctgtt gggccggtaa
     6961 tagcaaacag cccgttgctg gcgaacggct cgcgggtgaa atcaatcttc cattcgcctt
     7021 ttaatgagtt caggtttttc aggcgcaggc tgagaatttt catgcttcgt gttctccggc
     7081 gagggtatgc aacgtcgtgg tgaaaagatg ctgcagacgt tgctgctgcg attcatccag
     7141 ttcttccagt gccagacggc gattgaacac ctcttcgacg ctgagttcgc tgagggtttc
     7201 acgctgttgg ctggctaaca cacgctcgcg ctgttcacga ctccgacgta ccagcaatac
     7261 ttcgacaggc aatgattcgg ttaatgcctg gattttgcgc tgaatatcat gcagatactc
     7321 atcagtagtg atttcgatat ccagccagac aggtggctcc tgcgatacat cgcgccactg
     7381 ttccagctgt gcggtaatcg acgccagatc gcctttcagc actgccatgg gttgcgttac
     7441 cggtacgttc aggttttcca cgctctctaa tttgccgttt gaaaatgtca ccagatggac
     7501 atatttactc ttaccgcatt catcaaaact cagtggaatg ggggagccgc aatagcgaac
     7561 atgttccatg ccgccaataa tctgtgcgcg gtgaatatgc ccgagcgcga tgtagtcggc
     7621 tggtggaaag ttttgtgccg gaaacgcgtc cagcgtgcca atataaatgt cacgcacggc
     7681 gtcactttta ctggccccca cggtcgttaa atgtcccgtg gcgatgatgg gcagaggctg
     7741 atcgccgcgc agtttgcagg catcggcata gtgttgttgg taataatcgg taatcgctgc
     7801 cagtaaatgc tgctgttttt caataccgtt aagccccgcc tggctggtaa taatgtcacg
     7861 tggacgtaaa aacggaatgg ggcacagcac tgcgcctggc gtcccgtcgc gacgaggcaa
     7921 gatttgcggc gcatgtccgg cgctggcgac cacggtagta ttgaggaacg ccatgatatc
     7981 gcgcgattca ttcagcgtgg cgaccgagtc atggtttcct gccagtacca ccagatgaca
     8041 gccagtttgc tgtaaattga caacaaaacg gttgtataac gtgcgggcgt aactgggcgg
     8101 cgagccggta tcgaaaacat caccggcaac aataatcgca tccacctgat gggtttgtgc
     8161 tgtctccagc agccagtcaa gaaaagcctg atgttcagct tcgcggcttt tactgtagaa
     8221 gttctggccg agatgccagt ctgaggtgtg aaggatgcgc ataacggttc cctggcgaaa
     8281 aagcatgggc gcgattatac ccaaacagat gtgccatttg cttttttctg cgccacggaa
     8341 atcaataacc tgaagatatg tgcgacgagc ttttcataaa tctgtcataa atctgacgca
     8401 taatgacgtc gcattaatga tcgcaaccta tttattacaa cagggcaaat catgacgaga
     8461 cgtattctgg tcgtagaaga tgaagctcca attcgcgaaa tggtctgctt cgtgctcgaa
     8521 caaaatggct ttcagccggt cgaagcggaa gattatgaca gtgctgtgaa tcaactgaat
     8581 gaaccctggc cggatttaat tctccttgac tggatgttac ctggcggctc cggtatccag
     8641 ttcatcaaac acctcaagcg cgagtcgatg acccgggata ttccagtggt gatgttgacc
     8701 gccagagggg aagaagaaga tcgcgtgcgc ggccttgaaa ccggcgcgga tgactatatc
     8761 accaagccgt tttcgccgaa ggagctggtg gcgcgaatca aagcggtaat gcgccgtatt
     8821 tcgccaatgg cggtggaaga ggtgattgag atgcagggat taagtctcga cccgacatct
     8881 caccgagtga tggcgggcga agagccgctg gagatggggc cgacagaatt taaactgctg
     8941 cactttttta tgacgcatcc tgagcgcgtg tacagccgcg agcagctgtt aaaccacgtc
     9001 tggggaacta acgtgtatgt ggaagaccgc acggtcgatg tccacattcg tcgcctgcgt
     9061 aaagcactgg agcccggcgg gcatgaccgc atggtgcaga ccgtgcgcgg tacaggatat
     9121 cgtttttcaa cccgctttta acgccttgct catcggacgc agagcagggc ttatgatttc
     9181 ttaactggag tatcttacgt gctggaacgg ctgtcgtgga aaaggctggt gctggagctg
     9241 ctactttgct gcctcccggc tttcatcctg ggtgcatttt ttggttacct gccctggttt
     9301 ttgctggcat cggtaacggg actgcttatc tggcatttct ggaatttatt gcgcctttca
     9361 tggtggctgt gggtggatcg tagtatgacc ccgccaccgg ggcgtggtag ctgggaaccg
     9421 ctactatacg gcttacacca gatgcagctg cgaaataaaa aacgccgccg tgaactgggc
     9481 aatctgatta aacgctttcg tagcggcgcg gagtcgctgc ccgacgcagt ggtgctgacc
     9541 acggaagagg gcggtatttt ctggtgtaac ggtctggcgc aacaaattct tggtttgcgc
     9601 tggccggaag ataacgggca gaacatcctt aacctgctgc gttacccgga gtttacgcaa
     9661 tatctgaaaa cgcgtgattt ttctcgcccg ctcaatctgg tgctcaacac cgggcggcat
     9721 ctggaaattc gcgtcatgcc ttatacccac aaacagttgc tgatggtggc gcgtgatgtc
     9781 acgcaaatgc atcaactgga aggggcgcgg cgtaactttt ttgccaacgt gagccatgag
     9841 ttacgtacgc cattgaccgt gttacagggt tacctggaga tgatggatga gcagccgctg
     9901 gaaggcgcgg tacgcgaaaa agcgttgcac accatgcgcg agcagaccca gcggatggaa
     9961 gggctggtga agcagttgct aacgctgtcg aaaattgaag ccgcgccgac gcaattgctc
    10021 aatgaaaagg ttgatgtgcc gatgatgctg cgcgttgttg agcgcgaggc tcagactctg
    10081 agtcagaaaa aacagacatt tacctttgag atagataacg gcctcaaggt gtctggcaac
    10141 gaagatcagc tacgcagtgc gatttcgaac ctggtctata acgccgtgaa tcatacgccg
    10201 gaaggcacgc atatcaccgt acgctggctg cgagtgccgc acggtgccga atttagcgtt
    10261 gaagataacg gaccgggcat tgcaccggag catattccgc gcctgaccga gcgtttttat
    10321 cgcgttgata aagcgcgttc ccggcaaacc ggcggtagcg gattagggtt agcgatcgtg
    10381 aaacatgcgg tgaatcatca cgaaagtcgc ctgaatattg agagtacagt aggaaaagga
    10441 acacgtttca gttttgttat cccggaacgt ttaattgcca aaaacagcga ttaatccgcc
    10501 tttgtcatct tttattgcca taagccagcc gatgctggct tattttcttt gcagtcaaaa
    10561 tacgggcgtt agattttaca acggttggtg attttttgtt cgcatgatta gccatgtctt
    10621 tttcacggaa atagtgtttt atactggttg gtgatttctt atcgctatat acctctggtt
    10681 tttagatccc tccttgcttt aaaacgttat aagcgtttaa attgcgcttc aggtgctgtc
    10741 atactgactg cattaacgcg gtaaatcgaa aaactattct tcgccgcgcc tgattgggag
    10801 tatttcccgc taaaattgtt taaatatacc gctgtatcat ccccagggat tggcacaaaa
    10861 atttaacgtt acaacaccac atccacaggc agtatgattt atgacccatc aattaagatc
    10921 gcgcgatatc atcgctctgg gctttatgac atttgcgttg ttcgtcggcg caggtaacat
    10981 tattttccct ccaatggtcg gcttacaggc aggcgaacac gtctggactg cggcattcgg
    11041 cttcctcatt actgccgttg gcctgccggt attaacggta gtggcgctgg caaaagttgg
    11101 cggcggtgtt gacagcctca gcacgccaat cggtaaagtc gctggcgtac tgctggcaac
    11161 ggtttgttac ctggcggtgg ggccgctttt cgctacgccg cgtacagcta ccgtttcctt
    11221 tgaagtgggg attgcgccgc tgacgggtga ttccgcgctg ccgctgttta tctacagcct
    11281 ggtctatttc gctatcgtta ttctggtttc gctctatccg ggcaagctgc tggataccgt
    11341 gggcaacttc cttgcgccgc tgaaaattat cgcgctggtc atcctgtctg ttgccgcgat
    11401 tgtctggccg gcgggttcta tcagcacggc gactgaggct tatcaaaacg ctgcgttttc
    11461 taacggcttc gttaacggct atctgaccat ggatacgctg ggcgcaatgg tgtttggtat
    11521 cgttattgtt aacgcggcgc gttctcgtgg cgttaccgaa gcgcgtctgc tgacccgtta
    11581 taccgtctgg gctggcctga tggcgggtgt tggtctgact ctgctgtacc tggcgctgtt
    11641 ccgtctgggt tcagacagcg cgtcgctggt cgatcagtct gcaaacggcg ctgctattct
    11701 gcatgcttac gttcagcaca cctttggcgg cggcggtagc ttcctgctgg cggcgttaat
    11761 cttcatcgcc tgcctggtaa cggcagttgg cctgacctgt gcttgtgcag aattctttgc
    11821 ccagtacgta ccgctctctt atcgtacgct ggtgtttatc ctcggcggct tctcgatggt
    11881 ggtttctaac ctcggcttaa gccagctgat ccagatctcc gtaccggtgc tgaccgctat
    11941 ttatccgccg tgtatcgcac tggttgtatt aagttttaca cgctcatggt ggcataattc
    12001 gtcccgcgtg attgctccgc cgatgtttat cagcctgctt tttggtattc tcgacgggat
    12061 caaagcatct gcattcagcg atatcttacc gtcctgggcg cagcgtttac cgctggccga
    12121 acaaggtctg gcgtggttaa tgccaacagt ggtgatggtg gttctggcca ttatctggga
    12181 tcgcgcggca ggtcgtcagg tgacctccag cgctcactaa atcactgaac atttgtttta
    12241 accacggggc tgcgatgccc cgtggttttt tattgtgttg atgggttagg aattgatgga
    12301 aagtaagaac aagctaaagc gtgggctaag tacccgccac atacgcttta tggcactggg
    12361 ttcagcaatt ggcaccgggc tgttttacgg ttcggcagac gccatcaaaa tggccggtcc
    12421 gagcgtgttg ttggcctata ttatcggtgg tatcgcggcg tatatcatta tgcgtgcgct
    12481 gggggaaatg tcggtacata acccggcagc cagctctttc tcgcgttatg cgcaggaaaa
    12541 cctcggcccg ctggcaggtt acattaccgg ctggacctat tgctttgaaa tccttattgt
    12601 cgccatcgcc gatgtgaccg cttttggtat ctatatgggt gtctggttcc cgacggtgcc
    12661 gcactggatt tgggtactga gcgtggtgct gatcatttgc gccgtaaacc tgatgagcgt
    12721 gaaggtcttc ggtgagctgg aattctggtt ctcgttcttt aaagtcgcca ccatcatcat
    12781 catgattgtc gccggtttcg gcatcatcat ctgggggatt ggcaacggcg ggcaaccgac
    12841 cggtattcat aacctgtgga gcaacggcgg cttcttcagt aacggctggc ttgggatggt
    12901 gatgtcgttg caaatggtga tgtttgctta cggtgggatc gaaattatcg ggattaccgc
    12961 cggtgaagcg aaagatcctg agaaatcgat accgcgtgcg attaactccg tgccgatgcg
    13021 tattctggtg ttctacgtcg gtacgctgtt cgtcattatg tctatctacc cgtggaatca
    13081 ggttggcact gccggtagcc cgttcgtgct gacgttccag catatgggca ttacctttgc
    13141 cgccagcatt cttaactttg ttgtgctgac tgcttcgctg tcggcaatta acagtgacgt
    13201 atttggcgta ggccgtatgc tccacggtat ggcagagcag ggcagcgcgc cgaaaatttt
    13261 cagcaaaacg tcgcgtcgcg gtattccgtg ggttacggtg ctggtgatga ctaccgcgct
    13321 gctgtttgcg gtgtatctga actacatcat gccggaaaac gtcttcctgg tgatcgcttc
    13381 gctggcaacc ttcgccacgg tgtgggtgtg gattatgatc ctgctgtcgc aaattgcctt
    13441 ccgtcgccgt ttgccgccag aagaagttaa ggcgctgaaa tttaaagtgc cgggtggggt
    13501 agcaacgacc atcggcgggc tgattttcct gctctttatt atcgggttga ttggttatca
    13561 cccggatacg cgtatctcgc tgtatgtcgg tttcgcgtgg attgttgtgc tgttgattgg
    13621 ctggatgttt aaacgccgcc acgatcgtca gctggctgaa aaccaataat acttgcgttc
    13681 tgtaagccgg ttcaggagga aatcctgatc cggctattcc gaaagttatc cgcccccgtc
    13741 ctcctccccc aaatatcctt cagatgatga gtgatcctgc attaggctat ggcaaggtga
    13801 tcagattttc atcacagggg aattatgatg ttaaatgcat ggcacctgcc ggtgccccca
    13861 tttgttaaac aaagcaaaga tcaactgctc attacactgt ggctgacggg cgaagaccca
    13921 ccgcagcgca ttatgctgcg tacagaacac gataacgaag aaatgtcagt atcgatgcat
    13981 aagcagcgca gtcagcagca gcctggcgtc accgcatggc gtgcggcgat tgatctctcc
    14041 agcggacaac cccggcggcg ttacagtttc aaactgctgt ggcacgatcg ccagcgttgg
    14101 tttacaccgc agggcttcag ccgaatgccg ccggcacgac tggagcagtt tgccgtcgat
    14161 gtaccggata tcggcccaca atgggctgcg gatcagattt tttatcagat cttccctgat
    14221 cgttttgcgc gtagtcttcc tcgtgaagct gaacaggatc atgtctatta ccatcatgca
    14281 gccggacaag agatcatctt gcgtgactgg gatgaaccgg tcacggcgca ggcgggcgga
    14341 tcaacgttct atggcggcga tctggacggg ataagcgaaa aactgccgta tctgaaaaag
    14401 cttggcgtga cggcgctgta tctcaatccg gtgtttaaag ctcccagcgt acataaatac
    14461 gataccgagg attatcgcca tgtcgatccg cagtttgacg gtgatggggc gttgctgcgc
    14521 ttacgccaca atacgcagca gctgggaatg cggctggtgc tggatggcgt gtttaaccac
    14581 agtggcgatt cccatgcctg gtttgacagg cacaatcgtg gcacgggggg agcttgtcac
    14641 aaccctgaat cgccctggcg cgactggtac tcgtttagtg atgatggcac ggcgctcgac
    14701 tggcttggct atgccagctt gccgaagctg gattatcagt cggaaagtct ggtgaatgaa
    14761 atttatcgcg gggaagacag tattgtccgc cattggctga aagcgccgtg gagtatggac
    14821 ggctggcggc tgga
//
LOCUS       DAZKHF020000106        14809 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2201S31241
            SAMN43267800-rid25106413.denovo.107, whole genome shotgun sequence.
ACCESSION   DAZKHF020000106 DAZKHF020000000
VERSION     DAZKHF020000106.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267800
            Sequence Read Archive: SRR30307263
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 14809)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14809)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 14809)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350581.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350581.2
            Genome Coverage       :: 151x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:21:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,652
            CDSs (total)                      :: 5,557
            Genes (coding)                    :: 5,320
            CDSs (with protein)               :: 5,320
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 237
            CDSs (without protein)            :: 237
            Pseudo Genes (ambiguous residues) :: 0 of 237
            Pseudo Genes (frameshifted)       :: 102 of 237
            Pseudo Genes (incomplete)         :: 130 of 237
            Pseudo Genes (internal stop)      :: 52 of 237
            Pseudo Genes (multiple problems)  :: 41 of 237
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14809
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267800-rid25106413.denovo.107"
                     /serotype="O 26:H11"
                     /isolate="2201S31241"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-01"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            complement(1..692)
                     /locus_tag="ACHOSC_RS13045"
                     /old_locus_tag="pgaptmp_002609"
                     /pseudo
     CDS             complement(1..692)
                     /locus_tag="ACHOSC_RS13045"
                     /old_locus_tag="pgaptmp_002609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308264.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type IV secretion protein Rhs"
     gene            693..1430
                     /gene="tagH"
                     /locus_tag="ACHOSC_RS13050"
                     /old_locus_tag="pgaptmp_002610"
                     /pseudo
     CDS             693..1430
                     /gene="tagH"
                     /locus_tag="ACHOSC_RS13050"
                     /old_locus_tag="pgaptmp_002610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308254.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system-associated FHA domain
                     protein TagH"
     gene            1427..1951
                     /gene="tssJ"
                     /locus_tag="ACHOSC_RS13055"
                     /old_locus_tag="pgaptmp_002611"
     CDS             1427..1951
                     /gene="tssJ"
                     /locus_tag="ACHOSC_RS13055"
                     /old_locus_tag="pgaptmp_002611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system lipoprotein TssJ"
                     /protein_id="HGU4548529.1"
                     /translation="MNRKAFLACVLMCVILTGCETAKKISEVIKNPDIQVGSLKEQPS
                     EITVTLLTEPDTNTNAEGESAAVDVQLVYLTDDSKLLAADYDQIASTPLPDVLGKKYI
                     DHQDFNLLPDTIKTLPPVKLDEKTQFIGVVAYFSDDQATEWKQIETVEGTGHHYRLLV
                     HVRQSSIEMKKEDE"
     gene            1954..3284
                     /gene="tssK"
                     /locus_tag="ACHOSC_RS13060"
                     /old_locus_tag="pgaptmp_002612"
                     /pseudo
     CDS             1954..3284
                     /gene="tssK"
                     /locus_tag="ACHOSC_RS13060"
                     /old_locus_tag="pgaptmp_002612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308252.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system baseplate subunit TssK"
     gene            3289..4050
                     /gene="icmH"
                     /locus_tag="ACHOSC_RS13065"
                     /old_locus_tag="pgaptmp_002613"
     CDS             3289..4050
                     /gene="icmH"
                     /locus_tag="ACHOSC_RS13065"
                     /old_locus_tag="pgaptmp_002613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IVB secretion system protein IcmH/DotU"
                     /protein_id="HGU4548530.1"
                     /translation="MDEGSLSLPPFTGYDEKSQRDYHLALRGNSLNPMIDAATPLLGM
                     VMRLSTMNSQTMPEHLFAQVVTDVQAVEQLLQEQGYEPGVIISFRYILCTFIDEAALG
                     NGWSNKNEWIKQSLLVHFHNEAWGGEKVFILLERLIREPKRYQDLLEFLWLCFSLGFR
                     GRYKVAAQDQGEFEQIYRRLYHVLHKLRGDAPFPLLHQDKKTQGGRYQLISRLTIKHI
                     FCGGVVVLALFYLFYLLRLDSQTQDILHQLNKLLR"
     gene            4059..6878
                     /gene="tssH"
                     /locus_tag="ACHOSC_RS13070"
                     /old_locus_tag="pgaptmp_002614"
                     /gene_synonym="clpV"
     CDS             4059..6878
                     /gene="tssH"
                     /locus_tag="ACHOSC_RS13070"
                     /old_locus_tag="pgaptmp_002614"
                     /gene_synonym="clpV"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0033104 - type VI protein secretion
                     system complex [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system ATPase TssH"
                     /protein_id="HGU4548531.1"
                     /translation="MIQIDLPTLVKRLNLFSRQALEMAASECMSQQAAEITVSHVLIQ
                     MLAMPRSDLRVITRQGDIGMEELRQALTVENYTTARSADSYPAFSPMLVEWLKEGWLL
                     ASAEMQHSELRGGVLLLALLHSPLRYIPPAAARLLTGINRDRLQQDFVQWTQESAESV
                     VPDADGKGAGTLTDAADTLLARYAKNMTADARNGRLDPVLCRDHEIDLMIDILCRRRK
                     NNPVVVGEAGVGKSALIEGLALRIVAGHVPDKLKNTDIMTLDLGALQAGASVKGEFEK
                     RFKGLMAEVISSPVPVILFIDEAHTLIGAGNQQGGLDISNLLKPALARGELKTIAATT
                     WSEYKKYFEKDAALSRRFQLVKVSEPNAAEATIILRGLSAVYEQSHGVLIDDDALQAA
                     ATLSERYLSGRQLPDKAIDVLDTACARVAINLSSPPKQISALTTLSHQQEAEIRQLER
                     ELRIGLRTDTSRMTEVLVQYDETLTALDELEAAWHQQQTLVREIIALRQQLLGVAEDD
                     AAPLPDADTVEDTQPESESESESESESESESESESESEQDNTGAEPADEAGSEQPEET
                     AETVSPVQRLAQLTAELDALHNDRLLVSPHVDKKQIAAVIAEWTGVPLNRLSQNEMSV
                     ITDLTKWLGDTIKGQDLAIASLHKHLLTARADLRRPGRPLGAFLLAGPSGVGKTETVL
                     QLAELLYGGRQYLTTINMSEFQEKHTVSRLIGSPPGYVGYGEGGVLTEAIRQKPYSVV
                     LLDEVEKAHPDVLNLFYQAFDKGEMADGEGRLIDCKNIVFFLTSNLGYQVIVEHADDP
                     ETMQEVLYPVLADFFKPALLARMEVVPYLPLSKETLATIIAGKLARLDNVLRSRFGAE
                     VIIEPEVTDEIMSRVTRAENGARMLESVIDGNMLPPLSLLLLQKMAANTAVARIRLSA
                     VDGAFTADVEDAQNDESVTKDETVL"
     gene            6875..7618
                     /gene="vasI"
                     /locus_tag="ACHOSC_RS13075"
                     /old_locus_tag="pgaptmp_002615"
     CDS             6875..7618
                     /gene="vasI"
                     /locus_tag="ACHOSC_RS13075"
                     /old_locus_tag="pgaptmp_002615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system-associated protein
                     VasI"
                     /protein_id="HGU4548532.1"
                     /translation="MSRLRVFRQTGWFIAGLMTGLPATAAPAEASSMAGVAVAVATTT
                     PPDATATLQAMQSCRRESAALERLDCYDRLLAPLSPSGFDGALVKAGFVGEAWTRATE
                     QEKRREGNTTELLVTQVPGERPTVVITTPAIGHVPPRPVLMFSCVDNITRMQVALMHP
                     LDVHDIAVTLNADNRALRSHWFVRENGTLLESSRGLSGIDEIKQLFGAKTLTVDTGAD
                     NAAGKLTFNIDGLARAIAPLRDACHWAGE"
     gene            7623..9035
                     /gene="tssA"
                     /locus_tag="ACHOSC_RS13080"
                     /old_locus_tag="pgaptmp_002616"
                     /pseudo
     CDS             7623..9035
                     /gene="tssA"
                     /locus_tag="ACHOSC_RS13080"
                     /old_locus_tag="pgaptmp_002616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001743361.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system protein TssA"
     gene            9144..12578
                     /gene="tssM"
                     /locus_tag="ACHOSC_RS13085"
                     /old_locus_tag="pgaptmp_002617"
     CDS             9144..12578
                     /gene="tssM"
                     /locus_tag="ACHOSC_RS13085"
                     /old_locus_tag="pgaptmp_002617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308245.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system membrane subunit TssM"
                     /protein_id="HGU4548533.1"
                     /translation="MLILAWIFLLVWIWWKGPMWTLYEEQWLKPLANRWLATAAWGII
                     ALVWLTVRVMKRLQQLEKMQKQQREEAVDPLSVELNAQQRYLDRWLLRLQRHLDNRRF
                     LWQLPWYMVIGPAGSGKTTLLREGFPSDIIYAPEGARGAEQRLYLTPHVGKQAVIFDI
                     DGTLCAPADADILHRRLWEHALGWLKEKRARQPLNGIILTLDLPDLLTADKRRREHLL
                     QTLRSRLQDIRQHLHCQLPVYVVLTRLDLLQGFAALFQSLNRQDRDAILGVTFTRRAH
                     ENDDWRTELNAFWQTWVDRMNLALPDLMVAQTHTRASLFSFSRQMQGSREPLVSLLEG
                     LLDGENMNVMLRGVYLTSSLQRGQMDDIFTQSAARQYRLGNNPLASWPLVDTAPYFTR
                     SLFPQALLAEPNLATESRAWLIRSRRRLTVFSATGGVAALLLITGWHHYYNGNYQSGI
                     TVLKQAKAFMDVPPPQGEDDFGNLQLPLLNPVRDATLAYGDWGDRSRLADMGLYQGRR
                     IGPYVEQTYLQLLEQRYLPSLFNGLVKAMNAAPPESEEKLAVLRVMRMLEDKSGRNNE
                     VVKQYMAKRWSEKFHGQRDIQAQLMSHLDYALAHTDWHAERQAGDGDAISRWTPYDKP
                     VVSAQKELSKLPVYQRVYQSLKTRALGVLPADLNLRDQVGPTFDQVFTSADDNKLVVP
                     QFITRYGLQSYFVKQRDELVELTAMDSWVLNLTRNVKYSDADRAEIQHQLTEQYISDY
                     TATWRAGMDNLNIRNFESIGQLTGALEQVISGDQPLQRALTVLRDNTQPGVFSEKLSA
                     KEREEALAEPDYQLLTRLGHEFAPENSTLAVQKDKESTMQAVYQQLTELHRYLLAIQN
                     APVPGKSALKAVQLRLDQNSSDPIFATRQMAKTLPAPLNRWVGRLADQAWHVVMVEAV
                     HYMEVDWRDSVVKPFNEQLANNYPFNPRSAQDASLDAFERFFKPDGILDTFYQQNLKL
                     FIDNDLSLEDGDNNVIIREDIIAQLETAQKIRDIFFSKQNGLGTSFAVETVSLSGNKR
                     RSVLNLDGQLVDYSQGRNYTAHLVWPNNMREGNESKLTLIGTSGNAPRSISFSGPWAQ
                     FRLFGAGQLTGVQDGNFTVRFSVDGGAMTYRVHTDTEDNPFSGGLFSQFGLSDTLY"
     gene            12589..14038
                     /locus_tag="ACHOSC_RS13090"
                     /old_locus_tag="pgaptmp_002618"
                     /pseudo
     CDS             12589..14038
                     /locus_tag="ACHOSC_RS13090"
                     /old_locus_tag="pgaptmp_002618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308244.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="VasL domain-containing protein"
     gene            13965..14447
                     /locus_tag="ACHOSC_RS13095"
                     /old_locus_tag="pgaptmp_002619"
     CDS             13965..14447
                     /locus_tag="ACHOSC_RS13095"
                     /old_locus_tag="pgaptmp_002619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001284197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hcp family type VI secretion system effector"
                     /protein_id="HGU4548534.1"
                     /translation="MANISYLSLSGETQGLISAGCSTLDSVGNKAQPEHKDQIMVYAL
                     MHSISRSQNVNHHELIITKPVDKSSPLLAKALSDNEKMAICEFILYRTSKAGIYQPYY
                     KINLSKARISSIDFVTPHAVLEKELEPQERIAFIYEDISWEHTLAGTNAMSKWQDRVQ
                     "
     gene            14491..>14809
                     /locus_tag="ACHOSC_RS13100"
                     /old_locus_tag="pgaptmp_002620"
     CDS             14491..>14809
                     /locus_tag="ACHOSC_RS13100"
                     /old_locus_tag="pgaptmp_002620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000908053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4548535.1"
                     /translation="MAGLRNNNNTQNAQWADYVGDILRGAQPINQLVPQHPYLNDVPL
                     IDELRHQNTHHVILLTLDVAKKILSPITSFDYIHFITTHPSGIKDTLAWLVNAGKLMT
                     EFDD"
BASE COUNT         3429 a         4129 c         4194 g         3057 t
ORIGIN      
        1 ccgtagtgga ccgacacgcg gtggccttca ctggtatggc tgagggtggt taaccacccg
       61 tgctcatcat actgccactg ctctgccggg tcgccgttca ccgtccggtg cgtgatgcgg
      121 tccgacgcat cgtagtgcca gagggtgata agcccctcgt cttcactctg tgtgagtttt
      181 ctggtcaggt catagtggta acgttgcgtc cggccgtcaa aaccgcgctg ttcagtcagc
      241 ctgtcaaccg ggtcataccg gaacgtggac tggctgccgt tctcgttggt cagcacggtg
      301 atgcgcccgg cagcgtcata ccccatgctg cgcgtcagac cgccttccgt cacactcacc
      361 ggacgcccgc gtttatcata tgcgatggtg ctgcgtttac cgtccggtga taccgttgct
      421 gtcaggtctc ctgcggcgct gtactcataa cgggtttcac gcccctgcgc atccttctga
      481 ctgaccagtt gcccacgcgg gttataactg ctgtaagtgc tgatgccttc ctcccggtga
      541 acggcgattt gttgaccgta ccggtcgtac tcataccgcg tcgtgtaccc cgagcagtcc
      601 gtaaaggcca gcagttgacc gtaacggctc catgccatct gttttgtact gcccgtcgcg
      661 tcctgtatcc ctgtcggcag ctcactcgcc ggccgctttc aggcattgcg gctgtccggc
      721 catcggcacc gctgtccgat ccggcaagta atggggggat caatactccg tttatggatc
      781 tgccgcccat ttatgccagc cccggcgatc gtaatgacga cgtctctgcg gcagaaatgg
      841 cgcaacgcca ccttgcggtc acccccttac tgcgcggtct tggcggctcg cttaccatga
      901 gcaattccga cgatgcggat gattttctgg aggaggccgg acgaacgtta caggccgcaa
      961 taaaaggtct gctcgatttg cagcagcagc gtaacagcct ctcagacaaa catttgcgcc
     1021 cgctggaaga taacccgctg cgcctgaaca tggattacgc caccgcgctc gacgtaatgt
     1081 ttgccgaagg taaaagcccg gtacatctgg cggctcccgc cgccgtcagt gaaagcctgc
     1141 gcaatgtccg ccaccacgaa gaagctaacc gggcagcgat tgtggagtcg cttcgtgtcc
     1201 tgctggatgc tttctcacca caaaatctgc tgcgccgctt tgtgcagtac cgccgcagcc
     1261 atgaactgcg ccagccgctg gatgatgccg gagcatggca aatgtacagc cattattacg
     1321 aagaactggc ctccgatcgc cagcaggggt ttgagatgct gtttaacgag gtctacgccc
     1381 aagtctatga ccgggtgctt cgtgaaaaac agcgggagcc ggaagcatga acaggaaggc
     1441 gtttctggcc tgcgtgctga tgtgcgtcat attaaccggc tgcgaaacgg cgaaaaaaat
     1501 cagtgaggtg atcaaaaacc cggatatcca ggtcgggagc ctgaaggagc aaccttcgga
     1561 aataaccgtc accctgctga ccgaaccgga caccaatacc aatgcggaag gtgaatcagc
     1621 cgccgtggat gtgcaactgg tgtacctgac tgacgactca aaattgctgg cagccgacta
     1681 tgaccagata gccagtacgc cgctaccgga tgtgctgggt aaaaaatata tcgaccacca
     1741 ggatttcaac ctgttgccgg acaccatcaa aaccctgccg ccggtaaagc tggatgagaa
     1801 aacgcagttt attggtgtcg tggcctattt ctccgatgac caggccactg aatggaaaca
     1861 aatcgagacg gtggaaggaa ccggccacca ttaccgcctg ctggtgcatg tacgtcaaag
     1921 cagcatcgaa atgaaaaaag aggatgaata attatggcaa ccacccgcaa caaggtgatg
     1981 tggcaggaag ggatgctgat gcgtccacac catttccagc agcagcagcg ttacaacgac
     2041 tacctggata accagcgttt ccgggccatg aatgatttat cctggggatt taccgaactc
     2101 actctcaaca atgaactgct ggcacagggt aagatcatga ttgacagcgc gtcaggcaca
     2161 ctgcccgacg gcaccgtctt ttctatcccc gaccaggacg cactgcccga tccgctgcac
     2221 ccgcaacatt ttccggacga gagaagccgc aatatctacc tcgctctgcc ggtcgccagt
     2281 gatgtgagaa atgaaatcag cgacgggcgg cgaatcgggc gttaccggct gaattatgcc
     2341 gatgtccggg atttgcattc agaagaaggt gacacgcgaa cactgacgct gggacaactg
     2401 acgccgcgca tatgagcggt gcagaagata tgagcgccta tattacgctc ccgctttgcc
     2461 gtatcagcga tcgccatgcc gatggctccc tgacgttgga tgacgatttt atcccctcct
     2521 gccagaatat tcaggtcagt aagaaactgc gtgtttatct caaagaggta cagggggcca
     2581 ttggcggacg ggcaagcgat ctggcaaacc gtattggctc tccggcgcag agcggcatcg
     2641 cggatgtggc ggaatttatg atgttgcagt tacttaaccg taaccagacc cggtttaccc
     2701 atcgcgcccg ccgatcccag ctccacccgg aagatttcta ccttgatctt gctgggttgc
     2761 tgggtgaact gatgaccttt accgagccgt cgcgcctgcc ctgcccgctt gatgtgtatg
     2821 atcatcatga tctgaccaaa acatttaaaa cactgttacc ggaagtcaaa cgggcgctgc
     2881 ataccgtact gtcgccaaga gcggtcaatc tgccgctgca tctgcgtgac ggtatctggc
     2941 aggccgatat ccatgacaca gaactgctgc aatctgccac ctttgtgctg gccgtggcgg
     3001 caaacgtgcc ggtcgatcag atccagcgtc agtttatcca gcagtcgaaa atttcctcgc
     3061 cggaaaaaat ccgcaatatg gtcagtgtgc agatccccgg cattccattg cgcgcactga
     3121 tggtggctcc ccgccagctt ccttaccatt ccgggttcag ctatttcgaa ctcgacaaga
     3181 gcggacaggc ctggacagaa atggctgccg ccggggccgt tgcactgcat gtatccggca
     3241 gtttcccgga tctgaacatg caactgtggg cgataagagg gtaagacgat ggatgagggg
     3301 tcactgtctc tgccaccatt taccggatac gacgaaaaaa gccagcgcga ctatcatctc
     3361 gcgcttcgtg gcaacagcct gaatccgatg atcgacgcag ccacgccgct cctcggtatg
     3421 gtgatgcgcc tttcaaccat gaacagccag acgatgccgg agcatctttt tgctcaggtg
     3481 gtgacggatg ttcaggccgt ggagcaactg cttcaggagc agggctacga accgggcgtg
     3541 attatctcgt ttcgctacat cctctgcacg tttattgatg aagcggcgct cggtaacggc
     3601 tggtcgaaca agaacgagtg gatcaagcag tcattgctgg tccatttcca caacgaagcc
     3661 tggggtggag agaaagtttt catcctgctg gagcgcctga tccgtgaacc caaacgttac
     3721 caggatctgc tggaatttct ctggctctgt ttttctctgg gatttcgcgg gcgctacaaa
     3781 gtggcagcac aggatcaggg ggaatttgag cagatctacc gccgcctgta ccacgtcctg
     3841 cataaactac gcggcgatgc ccctttcccg ctgttacacc aggacaaaaa aacccagggc
     3901 gggcgttatc agctcatcag tcgcctgacc attaaacata ttttttgtgg tggcgttgtc
     3961 gtgctggcgt tgttttacct gttctatctg ttgcggctgg acagccagac gcaggacatc
     4021 ctgcaccagc taaacaaatt acttcgataa ggacgtttat gatccagatt gatcttccca
     4081 cgctggtaaa acggcttaac ctgttctccc gccaggcgct ggagatggcc gcctctgaat
     4141 gtatgagcca gcaggcagcg gaaattaccg tcagccatgt gcttattcag atgctcgcca
     4201 tgccacgcag tgacctgcgg gttattaccc gccagggcga tattggcatg gaagagttgc
     4261 gccaggcgct gacggtggag aactacacaa ctgcccgttc tgcggacagc tacccggcat
     4321 tttccccgat gctggttgag tggctgaaag agggctggct gctggcgtcg gctgagatgc
     4381 agcacagcga attgcgtggc ggcgtgttgc tgctggccct gctgcattcg ccgctgcgtt
     4441 atataccgcc tgctgccgcc cggctgttga ccggcattaa ccgtgaccgt ctgcaacagg
     4501 actttgtgca atggacacag gagtcggcgg aatcagtcgt gccggatgca gacggtaaag
     4561 gcgcaggcac actgacggac gctgctgaca ccctgcttgc ccgctacgcc aaaaacatga
     4621 ccgcagacgc ccgtaacggc aggcttgacc cggtactgtg ccgcgaccac gaaatcgacc
     4681 tgatgatcga cattctctgc cgccgccgca agaacaaccc ggtggtggtg ggcgaagcag
     4741 gcgtgggtaa aagcgcctta atcgaagggc tggcgctgcg catcgtggca ggccatgtgc
     4801 cggacaagct gaaaaacacc gatatcatga cccttgactt gggcgcattg caggccggag
     4861 cgtcggtgaa gggtgaattc gaaaaacgtt tcaaagggct gatggcagag gtcatttcct
     4921 ccccggtgcc ggtcattctg tttatcgacg aagcacatac cctgattggc gcgggcaacc
     4981 agcagggcgg gctggatatc tccaacctgc tcaaaccggc gctggcgcgc ggcgagctga
     5041 aaaccatcgc cgccaccacc tggagcgagt acaaaaaata cttcgaaaaa gatgccgccc
     5101 tgtcgcgccg cttccagttg gtgaaggtca gcgaacccaa cgctgccgaa gccaccatta
     5161 ttctgcgcgg tctgtcggcg gtctatgaac agtctcacgg cgtgctgatt gatgatgacg
     5221 ccttgcaggc cgctgcgaca ttaagcgagc gttatctctc cgggcgtcag ttaccggaca
     5281 aagcgattga tgtgctggat accgcctgcg cccgtgtggc catcaacctg tcgtcgccgc
     5341 cgaagcaaat ctcggcgctg accactctga gccaccagca ggaggcggaa attcgccagc
     5401 ttgagcgcga gcttcgcatc ggactgcgta ccgacacatc acggatgacc gaggtgctgg
     5461 tgcagtatga tgaaacgctg acggcgctgg atgaactgga agcggcctgg caccagcagc
     5521 agacgctggt ccgggagatt attgcgctgc gccagcagtt actgggcgtg gcagaggacg
     5581 atgcggcgcc gttgccggac gcagataccg tggaggatac gcagccagag tcagagtcag
     5641 agtcagagtc agagtcagag tcagagtcag agtcagagtc agagtcagag tcagagcagg
     5701 ataataccgg tgccgaaccg gctgatgaag ctggcagcga acagccggaa gagaccgctg
     5761 aaacagtttc cccggtacag cggctggccc agctcactgc cgaactggac gccctgcata
     5821 acgaccggtt gctggtctcc ccgcacgtcg ataaaaaaca gattgcggcg gtgattgccg
     5881 aatggaccgg cgtgccgctc aaccgcctat cgcagaatga aatgtcggtc atcaccgacc
     5941 tgacgaaatg gctgggtgac accatcaaag gccaggacct ggcgattgcc agcctgcata
     6001 agcatctact gaccgcacgc gccgacctgc gtcgtccggg acgcccgctc ggtgcgttcc
     6061 tgctggctgg ccccagcggt gtgggtaaaa ccgaaaccgt cctgcaactg gcagagctgc
     6121 tctacggcgg tcgccagtac ctgaccacca tcaatatgtc cgaattccag gagaaacaca
     6181 ccgtctcgcg gctgattggt tcgcctccgg gctacgttgg ctacggtgaa ggcggcgtac
     6241 tgaccgaagc gattcgccag aaaccctact cggtagtact gctcgatgaa gtggaaaaag
     6301 cgcacccgga tgtgctcaac ctgttctacc aggcgttcga taagggcgaa atggcagacg
     6361 gtgaaggccg cctgattgac tgcaaaaata tcgtcttctt cctgacgtcc aacctcggct
     6421 accaggtaat agtcgagcat gccgatgacc cggaaaccat gcaggaagta ctgtatccgg
     6481 tgctggccga cttcttcaaa cctgccctgc tggcgcgtat ggaagtggtg ccgtacctgc
     6541 cgctgtcgaa agagacgctc gccaccatca ttgccgggaa actggcccgc ctggataacg
     6601 tgctgcgcag ccgctttggc gcggaggtga ttatagaacc ggaagtgacg gacgaaatca
     6661 tgagccgcgt cacccgcgcg gaaaacggcg cgaggatgct ggaatcggtc atcgacggca
     6721 atatgctgcc gccgctctcc ctgctgctgt tgcagaaaat ggcggcgaat acggcggttg
     6781 cccggattcg gttgtcggca gtggacggcg catttacggc agacgtggaa gatgctcaga
     6841 acgacgagtc cgtcacaaag gatgaaacgg ttttatgagc aggttgcgcg ttttccggca
     6901 gacaggatgg tttatcgcgg gcctgatgac gggcttaccc gccactgccg cgcccgcaga
     6961 agcatcgtca atggccggtg tggcggtcgc cgtcgccacc acgaccccgc cggatgcgac
     7021 agccacgtta caggccatgc agtcctgtcg gcgggagtcc gcggcgctgg agcggcttga
     7081 ctgctatgac cgcctgctgg caccgctgtc cccgtcaggg tttgacgggg cgctggtcaa
     7141 agccggtttt gtgggcgagg cctggacccg cgccacagag caggaaaaac gccgcgaggg
     7201 caacaccaca gaactgctgg tgacgcaggt gccgggggag cgcccgacgg tggtgataac
     7261 caccccggct atcggtcatg ttccgccacg cccggtgctg atgttcagtt gcgtggacaa
     7321 tatcacccgg atgcaggtgg cgctgatgca ccctctggat gtccatgata tcgccgtcac
     7381 tctgaatgcc gacaaccggg cattgcgcag ccactggttt gtgcgcgaga acggcaccct
     7441 gctggagtcc agccgggggt tatccgggat tgatgaaatt aagcaactgt tcggcgcaaa
     7501 aacgctgacg gtggataccg gcgcagacaa tgccgccgga aagctgactt ttaacattga
     7561 cgggctggca agggcgattg ctcccctgcg cgatgcctgc cactgggcgg gagaataaaa
     7621 atatggcaat ggacttacgc gatccgaatg tctggatatc gcacctgctg gaaaacctgc
     7681 cggaagagaa actggcatcg gcgctgaaag atgacaaccc gaactgggag tatatcgacg
     7741 gcgaaatcgt caagctggga tcgctggccc acagtcaact ggatatcccg gaactccagc
     7801 gccgtgggct ggtcattctg gcttcggaga gtaaggattt tcgtctgctg gcgcatctgc
     7861 tgagaaccct gcaacacgcc ggtgatcctc atctcgcatt gcgcctgctg gcgctgtatg
     7921 tggagcatta ctgggcggtg gctgcgccgc agaatatggc gcataaaaaa cgctttgcca
     7981 gccaggtcat taaacgcttt gaaacaggta ttgaagtttt ttcacagaac gccgccacag
     8041 cgcagcgcga tgcgctgtca ggggaactgg caaaactggc gcaatgctgg cagtcacata
     8101 acgccccgga actggcacag gctactgatg atctttttgc cctgtaccag cgtgcgttta
     8161 atcgtgcggc accggctcct gtccccactc cggcggcctc cggtagttca ccacagacca
     8221 ccgccacgtc tgaaagcggc gtgacgcaac ccagtgctcc ggctccccaa atcgtcatcg
     8281 acagtcacga cgacaaagcc tagcgcgaca cgctgttaaa agtggcggcc attttatgtg
     8341 aacgccagcc ggattcaccg cagggctacc gtctgcgccg ccatgcgctt tggcagaaca
     8401 tcaccagtac gccccaggcg gaaagcgatg gccgtactcc actggctgcg gtctccgccg
     8461 atatggtggc agattaccat gctcagctcg gtagtgccga tatggcgctg tggcagcagg
     8521 tggagaaaag cgtactgctg gctccttact ggctggatgg tcactgcctt tctgcacaga
     8581 cggcgctgcg tctgggttac aaacaggtgg cagacgccat ccgcgatgag gtcatccgct
     8641 tccttgagcg tctgccccag cttaccgggc tgctgtttaa tgaccacaca ccgtttatca
     8701 gtgagcagac gaaacaatgg ctggcagctt cgcccgacgc caaagttgca ccggttgcgc
     8761 aaatcggtga ggaatcgaag gcagccagag cctgttttgc tgagcagggt ctggaggcgg
     8821 cgctgcgata tctggacatg ctacccgaag gcgatccccg cgatcagttt caccgccagt
     8881 accttgccgc acagttgacg gaagaggcgg ggctggtaca gcttgcgcag cagcagtacc
     8941 ggatgttgtt caggatggga ttgcagatga tggtggcaga ctgggagccg tccctgcttg
     9001 agcagctaga acaaaaattc acggcagaac aataaaagag aacaggagtt tttgtgttca
     9061 gattaccaac accccgacta ctcagcggac tcaaatcagc cctgcgaccg gcgatgcccc
     9121 ggtttaaagt ctctgctttc tggctgctga tactggcgtg gatttttctg ctggtgtgga
     9181 tctggtggaa aggcccgatg tggacgctgt atgaagaaca gtggctcaaa ccactggcga
     9241 accgctggct ggcaacggcg gcgtggggga ttattgccct ggtgtggctt accgtccggg
     9301 tgatgaagcg cctgcaacag cttgaaaaaa tgcaaaagca acagcgcgag gaagccgttg
     9361 atccgctcag cgtggaactg aacgcccagc agcgttatct tgaccgctgg ctgctgcgcc
     9421 tgcaacgcca tctcgacaac cgccgtttcc tgtggcagtt gccgtggtat atggtcatcg
     9481 gcccggcggg cagtggcaaa acgacactgc tgcgcgaggg gtttccgtcc gacattattt
     9541 atgccccgga gggcgcacgc ggcgcagaac aacgcctgta cctcacgccc catgtcggta
     9601 aacaggcggt gatctttgat atcgacggca cactctgcgc tcccgctgat gcggatatcc
     9661 tgcatcgccg tctgtgggaa catgccctcg gctggctgaa ggaaaagcgc gcacgccagc
     9721 cgctgaacgg gattattctg acactcgatt tacccgatct gctgaccgca gacaaacgcc
     9781 gccgcgagca tctgttacag acgctgcgca gccgcttaca ggatatacgc cagcatcttc
     9841 actgccagtt accggtttac gtggtactga cccggcttga tttattacag ggttttgccg
     9901 ccctgttcca gtcactcaac agacaggatc gcgatgcgat tctgggcgtc acgttcaccc
     9961 gccgcgccca tgaaaatgat gactggcgaa cagagttgaa tgctttctgg cagacatggg
    10021 tggatcgaat gaatctggcg ttgccggatc tgatggtcgc tcagactcac acccgcgcgt
    10081 ctttattcag tttttcccgc cagatgcagg gaagccgtga accgctggtg tcactgcttg
    10141 agggtctgct tgatggcgaa aatatgaacg tgatgctgcg tggtgtctat ctcacctctt
    10201 cgcttcagcg tgggcagatg gatgatatat tcacccagtc tgccgcccgc cagtaccggc
    10261 tgggcaataa cccactggcg tcctggcccc tggtggacac cgcgccttat ttcacccgca
    10321 gcctgttccc gcaggcatta ctcgcagagc ctaatctggc aacagagagc cgcgcctggc
    10381 tgatacgttc ccgtcgccgc ctgacggttt tctctgccac aggcggcgtg gcagcactgc
    10441 tgctcatcac cggctggcat cactattaca acggtaacta tcagtccggc atcaccgtgc
    10501 ttaagcaggc caaagccttt atggacgtgc cgcctccgca gggggaagat gactttggca
    10561 acctgcaact gccgctcctg aacccggtac gcgatgccac actggcctat ggcgactggg
    10621 gcgaccgcag ccgtctggcc gatatgggac tgtaccaggg acgacgtatc gggccttatg
    10681 tggaacagac ctatctgcaa ctgctggagc aacgttacct gccctcgctg tttaacgggc
    10741 tggtcaaagc gatgaacgcc gcgccgccgg agagtgaaga aaaactcgcg gtgctgcgcg
    10801 taatgcgaat gctggaggac aaaagcggac gtaacaatga ggtggtgaag cagtatatgg
    10861 caaaacgctg gagcgaaaaa ttccacggcc agcgcgatat ccaggcacaa ctgatgtccc
    10921 atcttgacta cgcgctggct catactgact ggcatgcaga gcgtcaggcg ggcgacggtg
    10981 acgccatcag ccgctggacg ccatatgaca agcccgtggt atcagcacag aaagaactga
    11041 gcaaactgcc tgtctaccag cgggtttacc agagcctgaa aacgcgggcg ctgggcgttc
    11101 ttcctgccga cctcaatctg cgtgaccagg tagggccaac ctttgaccag gtgtttacat
    11161 ctgccgatga caacaaactg gttgttccac agtttatcac ccgctacggc ctgcaaagct
    11221 attttgtaaa acagcgcgat gaactggttg aactgacagc gatggattcc tgggtactta
    11281 acctcacccg caatgtgaaa tacagtgacg ccgaccgtgc ggaaatccag caccagttga
    11341 ccgagcagta tatcagcgac tacaccgcca cctggcgggc tgggatggac aatctgaata
    11401 tccgcaattt tgagtccatc ggacaactga ccggggcgct ggagcaggtt atcagcggcg
    11461 accagccttt gcagcgggcg ctgaccgtgc tgcgtgacaa cacacagcca ggcgtctttt
    11521 ctgaaaaact ctctgccaaa gaacgggagg aagccctggc agagccggat taccagttac
    11581 tcacccgcct cgggcatgaa ttcgccccgg aaaacagtac cctggcagta cagaaagaca
    11641 aagaaagcac gatgcaggcc gtgtatcagc aactcaccga gttgcaccgc tacctgctgg
    11701 caatccagaa cgcgcctgta ccagggaaat cggcgctgaa agccgtgcag ttacggcttg
    11761 atcagaacag cagcgatccg atattcgcca cccgccagat ggcaaaaacg ctgcctgctc
    11821 cgctcaaccg ctgggttggc aggctggctg accaggcctg gcatgtggtg atggtggagg
    11881 ctgttcatta tatggaagtg gactggcgcg acagcgtggt gaaaccgttt aacgagcaac
    11941 tggcaaataa ctatccgttt aatccgcgtt ctgcacagga tgcctcactg gatgccttcg
    12001 aacgcttctt taaaccggat ggcatactgg ataccttcta ccagcagaac ctgaagctgt
    12061 ttatcgataa tgacctgagt ctggaggatg gcgataacaa cgtcattatt cgcgaagata
    12121 ttattgcgca actggaaact gcgcagaaaa tccgtgacat cttcttcagc aaacagaacg
    12181 gtctgggaac atcctttgcc gtggaaacgg tatcgctttc aggcaataaa cgccgcagtg
    12241 tactgaacct tgacggtcag ttagtcgatt acagccaggg ccgtaactat accgcccatc
    12301 tggtctggcc taacaacatg cgcgaaggca acgaaagtaa gctgacgctc atcggcacca
    12361 gcggcaacgc gccgcgcagt atcagcttca gcgggccgtg ggcgcagttc cgcctgttcg
    12421 gggccggaca actgaccgga gtacaggatg gcaactttac cgtgcgcttt agcgtggacg
    12481 gtggcgcgat gacctaccgt gtgcataccg acacggaaga taacccgttc agcggtgggt
    12541 tgttcagcca gtttggtctg tcagacacac tgtactgagg aggcagggat gaatagtaac
    12601 gtactgacac aaactatcgt taccggcagt gacccgcgcg ggctgccgga attcagcgcc
    12661 atccgcgagg aaataaacaa agccagccac ccgtcacagc ctgagctgaa ctggaaactg
    12721 gtggagtcgc tggcgctggc gatttttaaa gccaacggtg tggatttaca caccgccacc
    12781 tactatacgc ttgcccggac acggacacag ggactggcgg gattctgcga aggtgcggaa
    12841 ctgctggcgg caatggtaag ccacgactgg gataagttct ggccgcaggg cggcccggcg
    12901 cgtactgaaa tgctggactg gtttaactcc cgaaccggca atattctgcg tcagcaaatc
    12961 tcctttgcgg aatccgacct accactgatc taccgcacag agcgggcatt gcagcttatc
    13021 tgcgataagc tccagcaggt ggaactgaag cgcgttccgc gcgtggagaa tctgctctat
    13081 tttatgcaga acacgcgtaa acggcttgga ccccagctga agagtaacac tgagaacgcc
    13141 gcacagacca cggtcagaac gctgatttat gccccggaaa cacaggcatc ttccacacca
    13201 gaagcggtag tgcctcccct gcccggcctg cctgagatga aagtggaagt tcgcagtctg
    13261 acagagaatc ccccacaggc cagtgtgata aatcaaggca gtacggtaag agggtttatc
    13321 gcagggatcg cctgttcagt ggctgtcgcc tcagcattgt ggtggtggca ggtctatccg
    13381 gtgcagcagc aactgttaca ggttaacgac accgctcagg gcgcagcaac ggtgtggatg
    13441 gcctcacctg aactcgaaaa ctatgagcgc aggctgcaac aacttcttga tacctccccg
    13501 gtacagccgc tggaaaccgg gatgcagatg atgcgtgttg ccgacagtcg ctggccggaa
    13561 agcctgcaac agcaacaggc ctcgacacaa tggaatgagg cactcaaaac ccgcgcacag
    13621 agtagcccgc agttgcgtgg ctggttgcag acccgccagg acttacatgc ttttgcagat
    13681 ctagtgatgc agcgcgagaa agagggacta accctttcct atatcaaaaa tgtcatctgg
    13741 caggcggagc ggggactggg gcaggaaaca cccgttgagt ctctgttgac gcagtaccat
    13801 gatgcccgtg cgcagaagca gaatacagat gcgctggaaa aacaaattaa tgagcgactc
    13861 gaaggcgtgt taagccgctg gctgctgctg aagaataacg tcatgccaga ggcggcaacc
    13921 ggtaccacgg ccgaaaaata attatttaat taaaggagat tatcatggca aatataagtt
    13981 atttatcttt gagtggagag acacaagggc taatttcagc aggttgctca acgcttgatt
    14041 ctgtcggaaa caaagcgcag ccagaacata aagatcagat catggtttat gcgttaatgc
    14101 attccatctc aagaagtcaa aatgtcaatc atcatgaatt aattatcacc aaaccggttg
    14161 ataaatcctc accattgctg gcaaaagcac ttagtgacaa tgaaaaaatg gcaatatgtg
    14221 aatttatact ctaccgaacc agtaaagcgg gtatttacca gccttattat aaaataaatc
    14281 tgtcaaaagc ccgaatatca agtattgatt ttgtcacacc gcatgctgtc ctggaaaaag
    14341 aactggaacc acaggagaga attgccttta tttatgaaga tatttcctgg gagcatacgt
    14401 tagccggtac gaatgcaatg agtaaatggc aggatcgtgt acagtaactt actgtaataa
    14461 cataataaat tactattgcg gagagataaa atggctggtt taaggaataa caacaacaca
    14521 cagaatgcac agtgggctga ttatgtgggt gacattcttc ggggggctca gccgataaac
    14581 cagctagtac ctcagcaccc ttatcttaac gacgtcccac tcattgatga gttaaggcat
    14641 cagaacactc atcatgttat tctcttgaca cttgatgttg caaaaaaaat actcagccca
    14701 ataacatcat ttgattacat tcattttatc actacacatc cttcaggtat aaaagataca
    14761 ctcgcctggt tagttaacgc gggtaaatta atgactgagt ttgatgaca
//
LOCUS       DAZKHF020000107        14783 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2201S31241
            SAMN43267800-rid25106413.denovo.108, whole genome shotgun sequence.
ACCESSION   DAZKHF020000107 DAZKHF020000000
VERSION     DAZKHF020000107.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267800
            Sequence Read Archive: SRR30307263
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 14783)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14783)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 14783)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350581.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350581.2
            Genome Coverage       :: 151x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:21:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,652
            CDSs (total)                      :: 5,557
            Genes (coding)                    :: 5,320
            CDSs (with protein)               :: 5,320
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 237
            CDSs (without protein)            :: 237
            Pseudo Genes (ambiguous residues) :: 0 of 237
            Pseudo Genes (frameshifted)       :: 102 of 237
            Pseudo Genes (incomplete)         :: 130 of 237
            Pseudo Genes (internal stop)      :: 52 of 237
            Pseudo Genes (multiple problems)  :: 41 of 237
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14783
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267800-rid25106413.denovo.108"
                     /serotype="O 26:H11"
                     /isolate="2201S31241"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-01"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            complement(<1..393)
                     /locus_tag="ACHOSC_RS13105"
                     /old_locus_tag="pgaptmp_002621"
     CDS             complement(<1..393)
                     /locus_tag="ACHOSC_RS13105"
                     /old_locus_tag="pgaptmp_002621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ASCH domain-containing protein"
                     /protein_id="HGU4548536.1"
                     /translation="MTIKEKFSQKYPHASFCTFGDSAALADHLATLIATGVKTASCGS
                     LAGCIEDNAFPMIGEYKIVENSRGEPVCVIRVIGLHLLRFSDVTVELARKEGEGDLSL
                     EYWRNEHRRFFQAEGSYSPEMDVIFEEYA"
     gene            complement(692..2074)
                     /locus_tag="ACHOSC_RS13110"
                     /old_locus_tag="pgaptmp_002622"
     CDS             complement(692..2074)
                     /locus_tag="ACHOSC_RS13110"
                     /old_locus_tag="pgaptmp_002622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HGU4548537.1"
                     /translation="MKESNSRREFLSQSGKMVTAAALFGTSVPLAHAAVAGTLNCEAN
                     NTMKITDPHYYLDNVLLETGFDYENGVAVQTRTARQTVEIQDGKIVALRENKQHPDAT
                     LPHYDAGGKLMLPTTRDMHIHLDKTFYGGPWRSLNRPAGTTIQDMIKLEQKMLPELQP
                     YTQERAEKLIDLLQSKGTTIARSHCNIEPVSGLKNLQNLQAVLARRQAGFECEIVAFP
                     QHGLLLSKSEPLMREAMQAGAHYVGGLDPTSVDGAMEKSLDTMFQIALDYDKGVDIHL
                     HETTPSGVAAINYMVETVEKTPQLKGKLTISHAFALATLNEQQVDALANRMAAQQISI
                     ASTVPIGTLHMPLKQLHDKGVKVMTGTDSVIDHWSPYGLGDMLEKANLYAQLYIRPNE
                     QNLSRSLFLATGDVLPLNEKGERVWPKAQDDASFVLVDASCSAEAVARISPRTATFHK
                     GQLVWGSVAG"
     gene            complement(2084..3034)
                     /locus_tag="ACHOSC_RS13115"
                     /old_locus_tag="pgaptmp_002623"
     CDS             complement(2084..3034)
                     /locus_tag="ACHOSC_RS13115"
                     /old_locus_tag="pgaptmp_002623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamate kinase family protein"
                     /protein_id="HGU4548538.1"
                     /translation="MKELVVVAIGGNSIIKDNASQSIEHQAEAVKAVADTVLEMLASD
                     YDIVLTHGNGPQVGLDLRRAEIAHEREGLPLTPLANCVADTQGGIGYLIQQALNNRLA
                     RHGEKKAVTVVTQVEVDKNDPGFAHPTKPIGAFFSESQRDKLQKANPDWCFVEDAGRG
                     YRRVVASPEPKRIVEAPAIKALIQQGFVVIGAGGGGIPVVRTEAGDYQSVDAVIDKDL
                     STALLAREIHADILVITTGVEKVCIHFGKPQQQALDRVDIATMTRYMQEGHFPPGSML
                     PKIIASLTFLEQGGKEVIITTPECLPAALRGETGTHIIKT"
     gene            complement(3177..4595)
                     /locus_tag="ACHOSC_RS13120"
                     /old_locus_tag="pgaptmp_002624"
     CDS             complement(3177..4595)
                     /locus_tag="ACHOSC_RS13120"
                     /old_locus_tag="pgaptmp_002624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1116 domain-containing protein"
                     /protein_id="HGU4548539.1"
                     /translation="MSQSLFSQPLNVINVGIAMFSDDLKKQHVEVTQLDWTPPGQGNM
                     QVVQALDNIADSPLADKIAAANQQALERIIQSHPVLIGFDQAINVVPGMTPKTILHAG
                     PPITWEKMCGAMKGAVTGALVFEGLAKDLDEAAELAASGEITFSPCHEHDCVGSMAGV
                     TSASMFMHIVKNKTYGNIAYTNMSEQMAKILRMGANDQSVIDRLNWMRDVQGPMLRDA
                     MKIIGEIDLRLMLAQALHMGDECHNRNNAGTTLLIQALTPGIIQAGYSVEKQREVFEF
                     VASSDYFSGPTWMAMCKAAMDAAHGIEYSTVVTTMARNGVEFGLRVSGLPGQWFTGPA
                     QQVIGPMFAGYKPEDSGLDIGDSAITETYGIGGFAMATAPAIVALVGGTVEEAIDFSR
                     QMREITLGENPNVTIPLLGFMGVPSAIDITRVGSSGILPVINTAIAHKDAGVGMIGAG
                     IVHPPFACFEKAILGWCERYGV"
     gene            complement(4595..6142)
                     /gene="fdrA"
                     /locus_tag="ACHOSC_RS13125"
                     /old_locus_tag="pgaptmp_002625"
     CDS             complement(4595..6142)
                     /gene="fdrA"
                     /locus_tag="ACHOSC_RS13125"
                     /old_locus_tag="pgaptmp_002625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA synthetase FdrA"
                     /protein_id="HGU4548540.1"
                     /translation="MSVKIVIKPNTYFDSVSLMSISTRANKLDGVEQAFVAMATEMNK
                     GVLKNLGLLTPELEQAKNGDLMIVINGKSGADNEQLLVEIEELFNTKAQSGSHEARYA
                     TIASAKKHIPESNLAVISVNGLFAAREARQALQNDLNVMLFSDNVSVEDELALKQLAH
                     EKGLLMMGPDCGTAIINGAALCFGNAVRRGNIGIVGASGTGSQELSVRIHEFGGGVSQ
                     LIGTGGRDLSEKIGGLMMLDAIGMLENDPQTEIIALISKPPAPAVARKVLERARACRK
                     PVVVCFLGRVETPVDEQGLQFARGSKEAALKAVMLSGVKQENLDLHTLNQPLIADVRA
                     RLQPQQKYIRGLFCGGTLCDETMFAVMEKHGDVYSNIQPDPEFRLQDINRSIKHTFLD
                     FGDDDFTNGKPHPMIDPTNRISRLIEEARDPEVAVIVMDFVLGFGSHEDPVGSTIEAI
                     KEAKAIAAAEGRELIILAYVLGTDLDTPSLEQQSQMLLDAGVILASSSTNTGLLAREF
                     ICKGEEA"
     gene            complement(6132..6995)
                     /locus_tag="ACHOSC_RS13130"
                     /old_locus_tag="pgaptmp_002626"
     CDS             complement(6132..6995)
                     /locus_tag="ACHOSC_RS13130"
                     /old_locus_tag="pgaptmp_002626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2877 domain-containing protein"
                     /protein_id="HGU4548541.1"
                     /translation="MWALTADADFLAQRGQGQVEQVFARAVNIALPARQQLLTLLCEE
                     YDNAPNSCRLALTHFDDLFRHGDKVQFDDQGITVGQHLHIEMSRCRRWLSPTLQMTAV
                     NFHLIAWLQWHDIIHQHLGENETLFNYRGDNPFYQALNKELHIKRRAVIQAVNEKQNI
                     AAAVASMIGLGIGLTPSADDYLTGLALILFIPGHPSEKYKEEFYLGMQRGRNNTTLLS
                     AITLEAALQQRCRENIHRFIHNIIYDIPGNATQAIEKIKHIGSSSGCDMLYGMADGCA
                     LSQTYGGNYVS"
     gene            complement(7035..7640)
                     /locus_tag="ACHOSC_RS13135"
                     /old_locus_tag="pgaptmp_002627"
     CDS             complement(7035..7640)
                     /locus_tag="ACHOSC_RS13135"
                     /old_locus_tag="pgaptmp_002627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ankyrin repeat domain-containing protein"
                     /protein_id="HGU4548542.1"
                     /translation="MSIKNLPADYLLAAQQGDIDKVKTCLALGVDINTCDRQGKTAIT
                     LASLYQQYACVQALIDAGADINKQDHTCLNPFLISCLNDDLTLLRIILPAKPDLNCVT
                     RFGGVGLTPACEKGHLSIVKELLAHTEINVNQTNHVGWTPLLEAIVLNDGGIKQQAIV
                     QLLLEHGASPHLTDKYGKTPLELAREQGFEEIAQLLIAAGA"
     gene            7898..8395
                     /locus_tag="ACHOSC_RS13140"
                     /old_locus_tag="pgaptmp_002628"
     CDS             7898..8395
                     /locus_tag="ACHOSC_RS13140"
                     /old_locus_tag="pgaptmp_002628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1097 domain-containing protein"
                     /protein_id="HGU4548543.1"
                     /translation="MNGLTATGVTVGICAGLWQLVSSHVGLSQGWELLGTIGFVAFCS
                     FYAAGGGKSGFIRSLAVNYSGMVWAFFAALAAGWLASVSGLSAFWASVITTVPFSAVV
                     VWQGRFWLLSFIPGGFLGMTLFFASGMNWTVTLLGFLAGNCVGIISEYGGQKLSEATT
                     KRDGY"
     gene            8487..9419
                     /locus_tag="ACHOSC_RS13145"
                     /old_locus_tag="pgaptmp_002629"
     CDS             8487..9419
                     /locus_tag="ACHOSC_RS13145"
                     /old_locus_tag="pgaptmp_002629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR substrate-binding domain-containing
                     protein"
                     /protein_id="HGU4548544.1"
                     /translation="MNSIFTEENLLAFTTAARFGSFSKAAEELGLTTSAISYTIKRME
                     TGLDVVLFTRSTRSIELTESGRYFFRKATDLLNDFYAIKRSIDTISQGIEARVRICIN
                     QLLYTPKHTARLLQVLKKQFPTCQITVTTEVYNGVWDAIINNQANIAIGAPDTLLDGG
                     GIDYTEIGAIRWAFAIAPDHPLAFVPEPIAESQLRLYPNIMVEDTAHTINKKVGWLLH
                     GQESILVPDFNTKCQCQILGEGIGFLPDYMVREAMTQSLLVTRQIHNPRQDSRMLLAT
                     QHSATGQVTQWIKKQFAPNGILTGIYQDLLHREN"
     gene            complement(9461..10549)
                     /locus_tag="ACHOSC_RS13150"
                     /old_locus_tag="pgaptmp_002630"
     CDS             complement(9461..10549)
                     /locus_tag="ACHOSC_RS13150"
                     /old_locus_tag="pgaptmp_002630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HGU4548545.1"
                     /translation="MASHDLSVFLEEFGATVNLTLPGIVSEKERLLLKLLMEGMSVTE
                     ISQYRNRSAKTISHQKKQLYEKLGIQSDITFWRDIFFQYHPQVISGTGNKNNFYIPDN
                     RCHHIVTPEAISLALENHEFKPWIQPVFCAQTGVLTGCEVLVRWEHPQTGIIPPDQFI
                     PLAESSGLIVIMTRQLMKQTADILMPVKHLLPDNFHIGINVSAGCFLAAGFEKECLNL
                     VKKLGNDKIKLVLELTERNPIPVTPEARAIFDSLHQHNITFALDDFGTGYATYRYLQA
                     FPVDFIKIDKSFVQMASVDEISGHIVDNIVELARKPGLSIVAEGVETQEQADLMIGKG
                     VHFLQGYLYSPPVPGNKFISEWVMKAGG"
     gene            complement(10691..14674)
                     /gene="ehaA"
                     /locus_tag="ACHOSC_RS13155"
                     /old_locus_tag="pgaptmp_002631"
     CDS             complement(10691..14674)
                     /gene="ehaA"
                     /locus_tag="ACHOSC_RS13155"
                     /old_locus_tag="pgaptmp_002631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter adhesin EhaA"
                     /protein_id="HGU4548546.1"
                     /translation="MNKIYRLKWNRSRNCWSVCSELGSRVKGKKSRAVLISAISLYSS
                     LVFADDVIVNQDKTIDFGKENQSIDYRITVTDNANLVINTTDTSRPRLTLASGGGLDI
                     TGGKVTINGPLNFLLKGTGFLNVSNAGSELYADDLYESNSGMRHDQGYFNVSNGGKIH
                     VKGTSRLTYTQGNVSGEGSQVNAGTFFMGVYGGYGGNQFLSVNNGGEVNAREHISLGY
                     YDQRSDTTLVVSDGGKISAPKISLSTNSELALGAQEGSAAKAAGIIDAEKIEFVWAKT
                     SDKKITLNHTDKNATISADIVSGSEGLGYINALNGTTYLTGDNSAFSGKVKIEQNGAL
                     GITKNIGTAEINNRGKLHLKADDSMTFANKISGNGTISIDSGTVALTGNNYAFSGYID
                     VASGAVAVISEDKNIGRADLDVDGKLQINANKDWVFDNDLQGRGIVEINMGNHEFSFD
                     EFAYTDWFQGSLAFQNTTFNLEKNAEFLQRGGITAGQGSQVTVGKGAHSISTLGFSGG
                     TVDFGALTAGAQMTEGTVNVSKTLDLRGEGVIQVSDSDVVRSVSRDIDSALSLTEVDD
                     GNSAIKLVDAQGAEVLGDAGNLQLQDKNGQILSSSAQRDIQQNGQKAAVGTYDYRLTS
                     GVNNDGLYIGYGLTQLDLHATDSDALVLSSNGKSENAADLSAKITGSGDLAFSSQKGQ
                     TVSLSNKDNDYTGVTDLRSGTLLLNNDNVLGNTHELRLAAETELDMNGHSQTVGTLNG
                     SADSLLSLNGGSLTVTNGGTSTGSLTGSGELNIQGGTLDIAGDNSNLTANVNIANSAN
                     VLVSHAQGLGSANVENNGTLALNNSTEKRAAASVNYTLGGNLTNNGTLMTGMSGQQAG
                     NVLVVKGNYHGNNGQLVMNTVLNGDDSVTDKLVVEGDTSGTTAVTVNNAGGTGAKTLN
                     GIELIHVDGKSEGEFVQAGRIVAGAYDYTLARGQGANSGNWYLTSGSDSPELQPEPDP
                     MPNPEPNPNPEPNPNPTPTPGPDLNVDNDLRPEAGSYIANLAAANTMFTTRLHERLGN
                     TYYTDMVTGEQKQTTMWMRHEGGHNKWRDGSGQLKTQSNRYVLQLGGDVAQWSQNGSD
                     SWHVGVMAGYGNSDSKTISSRTGYRAKASVNGYSTGLYATWYADDESRNGAYLDSWAQ
                     YSWFDNTVKGDDLQSESYKSKGFTASLEAGYKHKLAEFNGSQGTRNEWYVQPQAQVTW
                     MGVKADKHRESNGTLVHSNGDGNVQTRLGVKTWLKSHHKMDDGKSREFQPFVEVNWLH
                     NSKDFSTSMDGVSVTQDGARNIAEIKTGVEGQLNANLNVWGNVGVQVADRGYNDTSAM
                     VGIKWQF"
BASE COUNT         3394 a         4031 c         3489 g         3869 t
ORIGIN      
        1 ggcatattct tcaaatataa cgtccatttc tggtgaatag cttccttctg cctggaaaaa
       61 acgacggtgt tcattacgcc aatattcaag actgaggtca ccttcacctt ccttacgggc
      121 aagctccacg gtgacatcag aaaagcgcag taaatgcagg ccaatcactc tgataacaca
      181 tacaggctca ccacggctat tttctacaat cttatactca ccaatcatcg gaaatgcgtt
      241 gtcttcaata catcccgcca gcgaaccaca ggaagctgtt ttaacaccag tcgcaatcaa
      301 cgttgcgagg tgatcggcca acgctgctga atcgccaaaa gtacaaaagg aagcatgggg
      361 atatttttgt gagaattttt cttttatggt catggcaata tttttgcgat tacggaaaaa
      421 caaatcacat cttaattact gttggtaacc atcagcaaga gtatcattgg tttttcgata
      481 ctgaatcttt tttatccgaa gcaggtcaca tttatggcct gataaatacc tttgttaaaa
      541 ctcacttttg tgtatttctc tcaaataatc ccctggaaat tatgccgcga gattataaag
      601 ttcaggatca ttcattagca aaccattccc gccccactcc ctggggctta aatctcttac
      661 aattcaccgt tgcaggcgtt tcaaatgcgc atcaacctgc cacactcccc cacaccagtt
      721 gccctttatg gaacgttgcg gttctcggcg agatacgcgc caccgcctcg gcggaacagg
      781 aggcgtccac cagcacaaag ctggcatcat cctgcgcttt tggccataca cgctcgcctt
      841 tttcattcag cggcaataca tcgccagtgg ctagaaacag cgaacgggag aggttctgtt
      901 cgttaggacg aatatagagc tgcgcgtaga gattggcttt ttccagcatg tcgcccagac
      961 cataaggcga ccagtggtcg ataacgctgt cagtgccagt catcactttt acgcctttgt
     1021 cgtgcaactg tttgagcggc atatgcagcg tgccaatcgg caccgtcgag gcgatagaaa
     1081 tttgctgcgc cgccatccga ttcgccagcg catcgacctg ttgttcgttg agcgtagcca
     1141 gcgcaaaggc gtggctgatg gtcagcttac ctttcagttg cggcgttttc tctaccgttt
     1201 caaccatata attgatggct gccacgcccg acggagtggt ttcgtgcagg tgaatatcga
     1261 cacctttgtc gtagtccagc gcaatctgaa acatggtgtc gagggatttt tccatcgcgc
     1321 catcgacact ggtcgggtcc agcccgccga cgtaatgcgc acccgcctgc atcgcttcgc
     1381 gcattaaggg ttccgatttc gacagcagca aaccgtgctg cgggaaggcg acgatttcac
     1441 actcgaagcc cgcctgacgt cgcgccagca ccgcctgcaa attctgcaga tttttcaggc
     1501 cggaaaccgg ttcgatattg cagtggctgc gggcaatggt ggtgcctttc gactgcaata
     1561 gatcgatcag tttttctgcc cgttcctgcg tgtacggttg cagttccggc agcattttct
     1621 gctcaagctt gatcatgtcc tggatagtgg tgcctgccgg acgattgagc gagcgccacg
     1681 gcccgccgta gaaggttttg tcgagatgaa tatgcatgtc gcgagtggtg ggtagcatca
     1741 acttaccgcc agcgtcatag tgcggcagcg tggcgtccgg atgctgcttg ttctcgcgca
     1801 gggcgacaat tttgccgtcc tgaatctcca cggtctggcg cgccgtgcgg gtctgcaccg
     1861 ccacaccgtt ttcgtagtca aaaccggttt ccagcagcac gttatcgaga tagtaatgcg
     1921 ggtcagtgat tttcatggtg ttgttcgctt cgcagtttag ggtgccagct accgccgcat
     1981 gggcgagcgg cacagaggta ccaaacagcg cggcggcggt gaccatctta ccgctctggc
     2041 tcagaaattc acggcggcta ttgctttctt tcatcctacg tccttacgtt ttaataatat
     2101 gggtgcccgt ttcaccgcgc agcgccgcag gcaggcattc cggcgtggtg ataatcactt
     2161 ctttgccgcc ctgctccagg aacgtcaggc tggcgatgat ttttggcaac atgctgccgg
     2221 gcggaaaatg cccttcctgc atatagcgag tcatggtggc aatatccacc cgatccagcg
     2281 cctgctgctg cggtttacca aagtggatac agactttttc gacgccggtg gtgatcacca
     2341 gaatgtcggc gtgaatttca cgggccagca gcgcggtaga gagatctttg tcaataaccg
     2401 cgtccacgct ttggtaatcc cccgcttcag tacgcactac cggaattcca ccgccgcccg
     2461 cgccaattac gacaaaacct tgttggatca gcgccttaat ggcgggtgct tcgacaatac
     2521 gtttcggttc cggcgaggcg accacgcggc gatagccccg tccggcatct tcaacaaaac
     2581 accagtcagg gtttgccttt tgtagtttgt cacgctggct ttcactgaag aatgcaccaa
     2641 tgggcttggt gggatgggca aaacctggat cgtttttatc cacttccacc tgagttacca
     2701 cggtgacggc ttttttctcg ccgtgacgcg ccagccggtt attcagcgcc tgttggatca
     2761 gatagccgat gccgccctgc gtatctgcca cacagttcgc cagcggcgtt aagggcagcc
     2821 cttcgcgctc gtgggcaatc tccgcacggc gtaaatccag cccgacctgc ggcccgttac
     2881 cgtgggtcag cacaatgtcg taatcggaag ccagcatttc caacaccgta tcggccacgg
     2941 ctttcaccgc ctccgcctga tgctcaatcg actggctggc gttatctttg ataatgctgt
     3001 tgccaccaat ggcaacgacc acaagctctt tcatgttgtt atgtccgcta cagagggtga
     3061 ttaggttatg accaacgcca gactgcctga tgcgctacgc ttatcaggcc tacaattctc
     3121 gggccatccg taggccggat aaggcgttca cgccgcatcc ggcatgaaca tgtaggtcag
     3181 acgccgtaac gttcgcacca gccaagaatg gctttctcga agcaggcaaa aggtggatgc
     3241 acaatgcccg cgccaatcat gccgacgccc gcatctttat gggcgatggc agtgttgatc
     3301 accggcagaa tgccgctgct acccacgcgg gtgatgtcga ttgccgacgg cacgcccata
     3361 aaaccgagca gcggaatggt gacgttgggg ttttcaccga gagtgatttc acgcatctgg
     3421 cgagagaaat cgatagcctc ttccaccgtg ccgcccacca gcgcaacgat tgcaggcgcg
     3481 gtcgccatag caaatccgcc aataccgtag gtttcagtga tggcgctgtc gccgatatcc
     3541 agccccgaat cttccggctt ataaccggca aacatcgggc cgatgacctg ctgcgccgga
     3601 ccggtaaacc attgccccgg taatccgctg acccgcaggc cgaactcgac gccgttacgc
     3661 gccatggtgg tgaccacggt gctgtattcg atgccgtgcg ccgcatccat cgccgcttta
     3721 cacatcgcca tccacgtcgg gccggagaag tagtcgctgc tagcgacaaa ctcaaacact
     3781 tcgcgctgtt tctccacgga ataacctgcc tgaataattc ccggcgtcag cgcctgaatc
     3841 agcaacgtcg tcccggcgtt attgcggtta tggcactcat cgcccatatg cagcgcctgc
     3901 gccagcatta agcgcagatc aatctcgccg ataattttca tcgcgtcgcg cagcattggc
     3961 ccctgcacat cgcgcatcca gttgaggcgg tcgatcacgc tctgatcgtt ggctcccata
     4021 cgcaaaatct tcgccatctg ctcgctcata ttggtgtaag cgatgttgcc gtaggttttg
     4081 ttttttacga tgtgcataaa catcgaggca gaggtaacac ccgccatcga tcccacacag
     4141 tcgtgttcgt ggcacggcga gaaggtgatc tccccggagg ccgccagttc agccgcctcg
     4201 tcgagatctt tcgccagccc ttcgaacacc aacgccccgg tgaccgcgcc tttcatcgcg
     4261 ccgcacattt tttcccaggt gatcggcggc ccggcgtgaa gaatggtttt cggcgtcatg
     4321 cccggcacca cgttaatcgc ctgatcaaaa ccaatcagca ccggatgcga ctggataatg
     4381 cgctccagcg cctgctggtt agcggcggcg attttgtctg ccagcggcga atcggcaatg
     4441 ttatccagcg cctgcaccac ctgcatattg ccctgccccg gcggcgtcca gtcgagttga
     4501 gtcacttcta catgctgctt tttcaggtca tcgctaaaca tggcgatgcc gacgttaata
     4561 acgttcaatg gttggctaaa cagtgactgg ctcatcaggc ttcctcccct ttgcagataa
     4621 attcacgcgc cagcaatccg gtattggtgc tgctgctcgc cagaatcact ccggcatcaa
     4681 gcagcatctg gctttgttgt tctaacgatg gcgtatcaag atcggtaccc agcacatagg
     4741 cgagaatgat caactcgcgt ccttcggcag cggcgatcgc tttcgcttct ttgatcgcct
     4801 cgatggtgga gccgaccgga tcttcatgcg atccaaatcc gagcacaaaa tccatcacga
     4861 tcaccgccac ttctggatcg cgcgcctctt cgatcaagcg actgatgcgg ttggtggggt
     4921 caatcattgg atgcggcttg ccgttcgtga agtcgtcatc gccaaagtcg aggaaggtgt
     4981 gtttgatgct gcggttgata tcttgcaggc ggaattccgg atcgggctga atgttgctgt
     5041 agacatcgcc atgtttttcc atcaccgcga acatggtttc gtcgcacagc gtgccgccgc
     5101 agaacaggcc acgaatgtat ttctgctgcg gttgcagacg cgcacgcaca tcagcaatca
     5161 acggctggtt aagcgtatgc aggtcgagat tttcctgttt cacgccggag agcatcaccg
     5221 cttttagcgc cgcctctttg ctgccgcggg caaactgtag cccctgctca tccaccggcg
     5281 tttcaacgcg accgaggaag cagaccacca ccggcttgcg gcaggcacgt gcgcgttcca
     5341 gcactttgcg ggccaccgca ggcgcgggag gttttgaaat cagtgcgata atttcagttt
     5401 gcggatcgtt ttccagcatc ccgatggcgt cgagcatcat caggccgccg attttctcgc
     5461 tcaggtcgcg cccgccggtg ccaatcagtt gtgaaacacc gccaccaaat tcatgaatgc
     5521 ggacgctcag ctcctggctg ccggtgccgg atgcgccaac aataccgata ttgccgcgac
     5581 gcacggcgtt accaaaacag agcgccgcgc cgttaataat cgccgtgccg cagtccggcc
     5641 ccatcatcag cagccctttt tcgtgggcca actgcttgag cgccagttca tcttcaactg
     5701 agacgttatc ggaaaacagc atcacgttga gatcgttttg cagcgcctga cgcgcttcgc
     5761 gagcggcaaa cagaccgttg accgaaatca ccgccaggtt actttccggg atatgctttt
     5821 tggcgctggc aatagtggcg taacgcgcct cgtgcgagcc gctttgcgct ttggtgttga
     5881 acagttcttc aatctccacc agcaactgct cgttgtccgc acccgattta ccattgatga
     5941 caatcatcag gtcgccgttt ttcgcctgtt ccagctccgg cgtcagcagt cctaaatttt
     6001 tcagtacacc tttgttcatt tcggtcgcca tcgccacaaa tgcctgctcg acgccgtcga
     6061 gtttatttgc acgcgtggag atagacatca gcgagacaga atcaaaatag gtattcggtt
     6121 taatgactat tttaactgac ataattccct ccgtaggttt ggctcagcgc acaaccatcg
     6181 gccatgccat acagcatgtc gcagccggaa ctggagccaa tatgtttaat tttttctatt
     6241 gcctgagttg cgttcccagg gatgtcataa ataatgttgt gaataaaacg atgaatattt
     6301 tcccggcaac gttgttgtaa tgcggcttcc agcgttatag cacttaataa tgtggtgtta
     6361 tttctgccgc gctgcatacc gagataaaat tcctctttgt atttttccga cggatgcccg
     6421 ggaataaata aaataagcgc cagacctgtt aaataatcgt cggctgatgg cgtaaggcca
     6481 atccctaacc caatcatact ggcgaccgct gcggcgatat tttgtttttc gtttactgcc
     6541 tgaataactg cccgtcgttt aatatgtagt tctttattta acgcctgata aaacggatta
     6601 tcgccgcgat aattaaacag ggtttcattt tcccccaggt gctgatgaat aatgtcgtgc
     6661 cactgtagcc aggcgataag gtgaaaattc acagcggtca tttgcaaggt tggggacagc
     6721 caacgccgac aacgactcat ctctatatga agatgttgac caaccgtaat accttgatcg
     6781 tcaaactgaa ccttatcacc atgccggaac agatcatcaa agtgagtgag tgccaaccga
     6841 caactgtttg gcgcattgtc gtactcttca caaagcagcg tcagcaactg ctggcgagcc
     6901 gggagtgcga tatttaccgc tctggcaaag acctgttcaa cctgtccttg cccccgctgc
     6961 gccagaaaat ccgcatccgc agttagcgcc cacacacacc gtctttctgg tgtgttgata
     7021 gcaagcctcc cggtttatgc acctgcggca atcagtaact gcgcaatctc ttcaaagccc
     7081 tgttcccgcg ccagttccag tggcgttttg ccatatttat cggtcagatg cgggctggca
     7141 ccgtgttcca gcaataactg cacaatcgcc tgctgtttaa taccaccatc attaagcaca
     7201 atcgcttcca gtagcggcgt ccagcctaca tggttggtct ggttaacgtt aatctccgta
     7261 tgcgccaaaa gctcttttac aatacttaaa tggccttttt cacaggcagg cgtcaggccg
     7321 acaccgccaa aacgggttac gcaattaaga tcgggtttag ccggtaaaat aattcgtagt
     7381 agcgttaaat catcgttcag acagctaatt aaaaaaggat ttaaacaggt atgatcttgc
     7441 ttattaatat ccgctccggc atcaattaat gcctgaacgc aagcatattg ctgatataaa
     7501 cttgccagcg taattgccgt tttcccctga cgatcgcagg tattaatatc gacacccagc
     7561 gcaaggcagg tttttacttt atcgatatca ccctgttgtg cagccaataa atagtcggca
     7621 ggtagatttt ttatactcat tcacattccc ctggtttttg tggctgtttt cgatgaattg
     7681 agaatagcaa cgataatctg ataaaagaac ccgcttaaat ttaattcgct gtatttcgcc
     7741 tttttgttga atgatgttca acaatctttt cgcagtgtgc ggtagtgcaa tatttgtttc
     7801 tttgacccga taccctttca ctcaatcagg ttttatttct ctatagtctg cacatgtaag
     7861 agtagctttc gtccatgtct aaccagaggg gaacaagatg aacggactca cggcaacggg
     7921 tgtcaccgtc ggtatttgtg cagggctttg gcagttagtg tcttcacatg tcgggctctc
     7981 tcagggatgg gaattattag gaaccattgg cttcgtcgcc ttttgcagtt tttatgccgc
     8041 aggcggcggt aaatcggggt ttatcagaag tctggcggtt aattattccg ggatggtgtg
     8101 ggcatttttc gccgcgctgg ctgccggttg gctggcctct gtaagtggac tgtccgcgtt
     8161 ctgggcgagc gtaattacta ccgtaccctt ctcggcggta gtggtctggc aagggcgttt
     8221 ctggttactg tcctttattc ccggtggctt tctcggcatg acgctgtttt ttgccagcgg
     8281 gatgaactgg acggtgacgt tactgggttt tctggcgggt aactgcgtgg ggataatttc
     8341 agaatatggt ggacaaaaat taagcgaagc gacgactaaa cgcgacgggt attaaaagtt
     8401 attcaaaaat gattatctat ctttaaccga tacctctaca gtcatagctg gagaaattca
     8461 tagtagataa tggcgagaat ggtttaatga actcaatttt taccgaagag aatctgttgg
     8521 catttactac cgctgcgcgt tttggcagct tcagtaaagc cgcagaagag ttgggtttaa
     8581 ccacttccgc cattagctac accatcaagc gtatggagac ggggctggat gtggtgctgt
     8641 tcactcgcag tacccgcagc attgagttaa cggagtccgg gcgttatttt ttccgcaaag
     8701 ctaccgacct gctgaatgat ttttatgcca tcaaacgcag tatcgatact atttcgcagg
     8761 gcatagaagc gcgtgtgcgc atttgtatta atcagctgct ttatacgccc aaacacaccg
     8821 cacgcttgtt acaggtgctg aaaaagcagt ttcctacctg ccagattacc gtcaccaccg
     8881 aagtgtataa cggcgtctgg gatgcgatta ttaataatca ggccaatatc gccatcggcg
     8941 cacccgatac actactggac ggcggcggta ttgattacac tgaaattggc gcgattcgct
     9001 gggcttttgc tatcgccccg gatcacccgt tagcctttgt gccggaaccc atcgccgaaa
     9061 gccaactgcg tctctaccct aatattatgg tggaggacac cgcgcatacg attaataaaa
     9121 aagtgggctg gttgctgcac gggcaggagt caattctggt gccagatttc aacaccaaat
     9181 gtcagtgcca gattttaggt gaaggaattg gttttttgcc ggattacatg gtccgtgagg
     9241 cgatgacgca atccctgctg gttacccgac aaatccataa tccgcgccag gattcgcgca
     9301 tgttactggc gactcagcat tctgctacgg gtcaggtgac gcagtggatt aaaaaacaat
     9361 ttgcgccaaa tggcatatta acggggatct atcaggattt actgcatcgg gagaattaat
     9421 cgctgccaac ctcccgatgc gctgtgaata gcggtttaca tcaaccacct gctttcatta
     9481 cccattcaga gataaattta ttacccggta ctggcggaga gtacaaatag ccctgcaaaa
     9541 agtgaacgcc tttaccgatc attaaatccg cctgctcctg ggtttctacc ccttccgcca
     9601 cgatactcag accaggctta cgcgctagtt cgacaatatt gtccacaata tgaccggaga
     9661 tttcgtcgac actcgccatt tgcacaaatg acttatcgat cttaataaaa tcgaccggga
     9721 acgcctgcaa gtaacgatag gtcgcataac ccgtaccaaa gtcatccagc gcaaaggtaa
     9781 tgttgtgctg atgaaggctg tcaaatatcg ctctggcttc tggcgttacc ggaatagggt
     9841 tacgttccgt tagctcgaga accagcttga ttttatcgtt acctaatttc ttaaccaggt
     9901 tcagacactc tttttcaaat cccgctgcca aaaaacaacc cgccgagacg ttgatgccaa
     9961 tatggaaatt gtccggcagc aaatgtttta ccggcatcag aatatccgca gtctgtttca
    10021 tcagttggcg ggtcattatg acaataagac cggatgactc cgccagagga ataaactgat
    10081 ccggtgggat aattcccgtt tgtggatgtt cccagcggac aagtacctca cagcccgtca
    10141 gtaccccagt ctgcgcgcag aaaaccggtt ggatccacgg tttgaattcg tggttttcca
    10201 acgccagact gatggcttca ggcgtgacga tatggtgaca acggttatca gggatataaa
    10261 aattattttt atttcccgtg ccactgatga cttgcggatg atattgaaaa aagatatccc
    10321 gccagaaggt aatatcactc tggatgccta gtttttcata cagctgcttc ttttgatgtg
    10381 aaattgtctt tgcactgcga tttctgtact gtgagatttc tgtaaccgac attccctcca
    10441 ttagcagctt gagtaaaagc cgttcttttt cggacacgat accaggcaac gtcaaattca
    10501 ccgttgcccc gaactcttcc aaaaaaacac tcaaatcatg cgaagccatt gaacacacct
    10561 tgattaaatc ttgtgcgcca gctaaatctg cgagtcttca ctctgactga taaagctggc
    10621 tgcaagaaaa tgacacagag agccgaatta tctcccgttc ggctctctct ccttttactc
    10681 attattcaga tcagaattgc cacttaatgc caaccattgc agaggtgtca ttatatcccc
    10741 tatcggcaac ctgaacgccc acattccccc agacattcag gttggcattt agctgtcctt
    10801 ccaccccggt ttttatctca gcaatatttc gggctccatc ctgagtgacg gacacgccat
    10861 ccatactggt gctgaaatcc ttactgttat gcagccagtt cacttctaca aacggctgga
    10921 actcgcggga tttaccgtca tccattttat ggtggctctt cagccaggtt tttacgccaa
    10981 gtcgggtttg aacattgcca tcaccgttgc tatgaacgag ggttccgttg ctttcgcggt
    11041 gcttatcggc tttgactccc atccaggtaa cctgtgcttg cggctgaaca taccattcat
    11101 tacgcgttcc ctggctgcca ttaaattcag ctaatttgtg tttgtatcca gcttccagtg
    11161 aagcggtaaa tccttttgat ttataggatt cactttgtaa gtcatcccct ttcactgtgt
    11221 tatcaaacca gctgtactgc gcccaactgt cgagatacgc gccattacgc gactcgtcat
    11281 cggcatacca ggtggcatag aggccggtgc tatatccgtt cacactcgct tttgcacgat
    11341 aaccggttcg cgaggaaatg gttttgctgt cgctgttgcc atatcccgcc atgaccccaa
    11401 catgccagct gtcgctgccg ttttggctcc actgcgcgac atcgcctccc agttgcagaa
    11461 catagcgatt gctttgggtt ttcagctggc cgctgccatc acgccattta ttatgaccac
    11521 cttcatggcg catccacata gtggtttgtt tctgctcacc cgtcaccatg tcggtatagt
    11581 acgtattacc cagacgctca tgcagacgcg tggtgaacat tgtattcgct gctgcaaggt
    11641 tcgcaatgta gctacccgcc tccggtcgca ggtcattatc cacattcaga tccggacccg
    11701 gcgtaggtgt cgggttaggg ttcggctctg gattcgggtt tggctccgga ttcggcatcg
    11761 ggtctggctc cggctgcagt tcaggagaat cactgccgct ggtcagatac cagttaccac
    11821 tatttgcccc ttgtccacgc gcgagagtgt agtcatacgc ccccgcaacg atacgaccag
    11881 cctgaacaaa ttcgccctca gacttaccgt ctacatggat aagttcgata ccgttaaggg
    11941 ttttcgcacc tgtaccgcca gcgttattca ccgtaacggc agtcgtgccg ctagtatcgc
    12001 cctcgacaac caatttatcg gttactgagt catcgccatt cagtaccgta ttcattacta
    12061 gttgaccgtt attaccgtgg tagttcccct tcaccactaa cacattgcca gcttgctgtc
    12121 ctgacattcc ggtcatcagc gtaccgttgt tggtcagatt gccgcccagg gtgtaattca
    12181 cagacgcagc cgctcttttt tcagtgctat tattcaacgc cagggtaccg ttgttctcaa
    12241 cgtttgcgct acccaatccc tgcgcatgac ttaccaggac attagccgaa ttagcaatgt
    12301 tcacattcgc cgtcaggttg ctgttatcgc ccgcgatgtc cagcgtgccg ccctgaatat
    12361 tcagctctcc gctccccgtt aacgaaccgg ttgaagtgcc cccgttggta accgtcagac
    12421 tgccgccatt taagctcagc agtgaatcgg cgctgccatt gagcgtgccc acggtctggc
    12481 tgtgaccatt catatccagt tcagtctctg ccgccagacg cagttcatgg gtattaccca
    12541 acacgttatc gttattcaac aaaagcgtcc cactgcgcag atcggtaaca ccggtatagt
    12601 cgttgtcttt gttagaaagc gatacggtct gacccttctg gctgctgaat gccaggtcac
    12661 cactgccggt aatctttgcg ctgagatcgg cggcattctc gcttttaccg ttagagctca
    12721 gcaccagagc atcgctgtcg gtagcgtgta aatcaagctg ggtcaggccg taaccgatat
    12781 acagaccgtc attgtttacc ccactcgtca gacgatagtc gtaagtgccg acggccgctt
    12841 tttgcccatt ctgctgaata tcacgttggg cgctgctgga gaggatttgc ccatttttat
    12901 cctgcaattg cagattgccc gcatcgccca gaacttccgc accttgcgca tcaaccagct
    12961 taatggcgct gttaccatca tcgacttcag tgagcgataa cgcagagtca atatcacgag
    13021 atactgagcg gacaacgtca ctgtcagaaa cctgaatcac accctcgccg cgcaaatcca
    13081 gcgttttact aacgttgacc gtcccttctg tcatctgtgc acctgctgtc agggcaccaa
    13141 aatcaacggt tccgccggag aatcccaaag tgctaatgga gtgagcgccc ttacccactg
    13201 ttacctggct tccctgaccc gcagtgatcc cgcctctctg cagaaactca gcattctttt
    13261 ccaggttaaa tgtcgtgttc tggaacgcca gtgaaccctg gaaccagtct gtataagcaa
    13321 actcatcgaa ggagaattcg tgattcccca tgtttatttc aacaatacct ctaccttgaa
    13381 gatcgttatc aaatacccaa tctttattgg cattgatttg cagtttgcca tcaacatcca
    13441 ggtccgcacg gccgatgttc ttgtcttcag aaataaccgc gacagcacca gaagcgacat
    13501 caatatatcc gctgaatgca tagttattgc cggtcagtgc cactgtccca ctgtcgatac
    13561 ttattgtacc attgccggaa atcttattgg caaaggtcat gctatcgtca gccttcaggt
    13621 gtaatttccc gcggttgttg atctctgctg tacctatatt tttggtgatc cctaaagcgc
    13681 cattttgctc aattttgact ttaccactaa aggcagagtt atcaccggtt aagtaagtcg
    13741 tgccattgag cgcattgata tagcccaggc cctcgctgcc actgacaata tccgcggaaa
    13801 tagtcgcgtt tttatccgtg tggtttaagg tgattttctt atcggatgtc tttgcccaca
    13861 caaactcaat tttttcggca tcaataatcc ctgcagcctt cgcagcactt ccttcctgtg
    13921 cacctaacgc taactcagag ttggtgctta aactaatttt cggtgcggat attttacctc
    13981 catctgatac gactaatgtt gtatcagaac gttggtcgta ataacccagg ctaatatgtt
    14041 cacgagcatt aacttcacca ccgttattaa ccgacaggaa ctgattaccg ccatatccgc
    14101 cgtaaacgcc cataaagaag gtccctgcat ttacctgact accttcgccg cttacattcc
    14161 cctgcgtata agtcagacga ctggtgccct taacatgaat tttgccgcca ttggagacat
    14221 taaaatagcc ttgatcatgt ctcatgcctg agtttgactc atacaaatca tcagcatata
    14281 actcgctgcc agcattggag acattcagga accccgtacc tttcagcaaa aagttaagcg
    14341 ggccattgat tgttaccttt cctccggtaa tatccaaccc accaccagaa gcgagagtca
    14401 gacgcggacg ggaagtatct gtcgtattga ttaccagatt ggcattgtca gtcaccgtaa
    14461 tacggtaatc gatgctctgg ttctctttgc caaaatcaat agttttatcc tggtttacga
    14521 tgacatcatc ggcgaatacc agagatgaat ataaacttat cgcgctaatt aaaacagccc
    14581 gggacttttt tccttttact ctgctcccca gctcagagca gacgctccaa cagttacggg
    14641 acctgttcca ctttagccga tatattttat tcatcacaat ctccagcaat acatccaaat
    14701 aaaaccaaga ttgcataaac agcaacgcat taaatgccat tctggttact gttcatcgca
    14761 tataaaaatt caaaaatttt gca
//
LOCUS       DAZKHF020000108        14748 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2201S31241
            SAMN43267800-rid25106413.denovo.109, whole genome shotgun sequence.
ACCESSION   DAZKHF020000108 DAZKHF020000000
VERSION     DAZKHF020000108.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267800
            Sequence Read Archive: SRR30307263
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 14748)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14748)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 14748)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350581.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350581.2
            Genome Coverage       :: 151x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:21:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,652
            CDSs (total)                      :: 5,557
            Genes (coding)                    :: 5,320
            CDSs (with protein)               :: 5,320
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 237
            CDSs (without protein)            :: 237
            Pseudo Genes (ambiguous residues) :: 0 of 237
            Pseudo Genes (frameshifted)       :: 102 of 237
            Pseudo Genes (incomplete)         :: 130 of 237
            Pseudo Genes (internal stop)      :: 52 of 237
            Pseudo Genes (multiple problems)  :: 41 of 237
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14748
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267800-rid25106413.denovo.109"
                     /serotype="O 26:H11"
                     /isolate="2201S31241"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-01"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            complement(80..1081)
                     /locus_tag="ACHOSC_RS13160"
                     /old_locus_tag="pgaptmp_002632"
     CDS             complement(80..1081)
                     /locus_tag="ACHOSC_RS13160"
                     /old_locus_tag="pgaptmp_002632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000100290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="permease"
                     /protein_id="HGU4548547.1"
                     /translation="MSSWLAMLQDAAEMFVFLAVELSLLFIVISAGVSLIRQKVPDHK
                     IQQMMGARKGRGYLLAALLGAVTPFCSCSTIPMLRGLLSAKAGFGPTLTFLFVSPLLN
                     PIIVGLMWMTFGWKVTLLYAIIAAGVSVLASIILDSLGFERHIIASKSSSANCCAPAK
                     TSPGTTYTPIKVSCCSPAAKAIENPVVTCCSTKAVVSINPIKLATKDALQQFKDVLPY
                     LLLGVLIGSFIYGFIPSAWIAAHAGADNPFAIPLSAVVGIPLYIRAEAVIPLASVLMT
                     KGMGLGALMALIIGSAGASLTEVILLKSMFRMPMIAAFLTVILGMAILMGYLTQLLF"
     gene            1188..1484
                     /locus_tag="ACHOSC_RS13165"
                     /old_locus_tag="pgaptmp_002633"
     CDS             1188..1484
                     /locus_tag="ACHOSC_RS13165"
                     /old_locus_tag="pgaptmp_002633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001465662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HGU4548548.1"
                     /translation="MQLEEVAKALKELGHPTRLFIFKHLVKAGEQGLPVGELQKQLGI
                     PGSTLSHHISALVSVGLVKQNRESRTLMCVSQYAMLEAIIEFLREECCVNSKIA"
     gene            complement(1618..2043)
                     /gene="arsC"
                     /locus_tag="ACHOSC_RS13170"
                     /old_locus_tag="pgaptmp_002634"
     CDS             complement(1618..2043)
                     /gene="arsC"
                     /locus_tag="ACHOSC_RS13170"
                     /old_locus_tag="pgaptmp_002634"
                     /EC_number="1.20.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008794 - arsenate reductase
                     (glutaredoxin) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin-dependent arsenate reductase"
                     /protein_id="HGU4548549.1"
                     /translation="MSNITIYHNPACGTSRNTLEMIRNSGTEPTIIHYLETPPTRDEL
                     VKLIADMGISVRALLRKNVEPYEELGLAEDKFTDDRLIDFMLQHPILINRPIVVTPLG
                     TRLCRPSEVVLEILPDAQKGAFSKEDGEKVVDEAGKRLK"
     gene            complement(2056..3345)
                     /gene="arsB"
                     /locus_tag="ACHOSC_RS13175"
                     /old_locus_tag="pgaptmp_002635"
     CDS             complement(2056..3345)
                     /gene="arsB"
                     /locus_tag="ACHOSC_RS13175"
                     /old_locus_tag="pgaptmp_002635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arsenite/antimonite:H(+) antiporter ArsB"
                     /protein_id="HGU4548550.1"
                     /translation="MLLAGAIFVLTIVLVIWQPKGLGIGWSATLGAVLALVTGVVHPG
                     DIPVVWNIVWNATAAFIAVIIISLLLDESGFFEWAALHVSRWGNGRGRLLFTWIVLLG
                     AAVAALFANDGAALILTPIVIAMLLALGFSKGTTLAFVMAAGFIADTASLPLIVSNLV
                     NIVSADFFGLGFREYASVMVPVDIAAIVATLVMLHLYFRKDIPQNYDMALLKSPAEAI
                     KDPATFKTGWVVLLLLLVGFFVLEPLGIPVSAIAAVGALILFVVAKRGHAINTGKVLR
                     GAPWQIVIFSLGMYLVVYGLRNAGLTEYLSGVLNVLADNGLWAATLGTGFLTAFLSSI
                     MNNMPTVLVGALSIDGSTASGVIKEAMVYANVIGCDLGPKITPIGSLATLLWLHVLSQ
                     KNMTISWGYYFRTGIIMTLPVLFVTLAALALRLSFTL"
     gene            complement(3399..3752)
                     /gene="arsR"
                     /locus_tag="ACHOSC_RS13180"
                     /old_locus_tag="pgaptmp_002636"
     CDS             complement(3399..3752)
                     /gene="arsR"
                     /locus_tag="ACHOSC_RS13180"
                     /old_locus_tag="pgaptmp_002636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="As(III)-sensing metalloregulatory
                     transcriptional repressor ArsR"
                     /protein_id="HGU4548551.1"
                     /translation="MSFLLPIQLFKILADETRLGIVLLLSELGELCVCDLCTALDQSQ
                     PKISRHLALLRESGLLLDRKQGKWVHYRLSPHIPAWAAKIIDEAWRCEQEKVQAIVRN
                     LARQNCSGDSKNICS"
     gene            4492..4575
                     /gene="dinQ"
                     /locus_tag="ACHOSC_RS13185"
                     /old_locus_tag="pgaptmp_002637"
     CDS             4492..4575
                     /gene="dinQ"
                     /locus_tag="ACHOSC_RS13185"
                     /old_locus_tag="pgaptmp_002637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165328.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="damage-inducible type I toxin DinQ"
                     /protein_id="HGU4548552.1"
                     /translation="MIDKAIIVLGALIALLELIRFLLQLLN"
     gene            complement(4629..5981)
                     /gene="gorA"
                     /locus_tag="ACHOSC_RS13190"
                     /old_locus_tag="pgaptmp_002638"
     CDS             complement(4629..5981)
                     /gene="gorA"
                     /locus_tag="ACHOSC_RS13190"
                     /old_locus_tag="pgaptmp_002638"
                     /EC_number="1.8.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003861215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004362 - glutathione-disulfide reductase
                     (NADPH) activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA];
                     GO_process: GO:0045454 - cell redox homeostasis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-disulfide reductase"
                     /protein_id="HGU4548553.1"
                     /translation="MTKHYDYIAIGGGSGGIASINRAAMYGQKCALIEAKELGGTCVN
                     VGCVPKKVMWHAAQIREAIHMYGPDYGFDTTINKFNWETLIASRTAYIDRIHTSYENV
                     LGKNNVDVIKGFARFVDAKTLEVNGETITADHILIATGGRPSHPDIPGVEYGIDSDGF
                     FALPALPERVAVVGAGYIAVELAGVINGLGAKTHLFVRKHAPLRSFDPMISETLVEVM
                     NAEGPQLHTNAIPKAVVKNADGSLTLELEDGRSETVDCLIWAIGREPANDNINLEAAG
                     VKTNEKGYIVVDKYQNTNIEGIYAVGDNTGAVELTPVAVAAGRRLSERLFNNKPDEHL
                     DYSNIPTVVFSHPPIGTVGLTEPQAREQYGDDQVKVYKSSFTAMYTAVTTHRQPCRMK
                     LVCVGSEEKIVGIHGIGFGMDEMLQGFAVALKMGATKKDFDNTVAIHPTAAEEFVTMR
                     "
     gene            complement(6053..6895)
                     /gene="rlmJ"
                     /locus_tag="ACHOSC_RS13195"
                     /old_locus_tag="pgaptmp_002639"
     CDS             complement(6053..6895)
                     /gene="rlmJ"
                     /locus_tag="ACHOSC_RS13195"
                     /old_locus_tag="pgaptmp_002639"
                     /EC_number="2.1.1.266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2030)-N(6))-methyltransferase"
                     /protein_id="HGU4548554.1"
                     /translation="MLSYRHSFHAGNHADVLKHTVQSLIIESLKEKDKPFLYLDTHAG
                     AGRYQLGSEHAERTGEYLEGIARIWQQDDLPAELEAYINVVKHFNRSGQLRYYPGSPL
                     IARQLLREQDSLQLTELHPSDYPLLRSEFQKDSRARVEKADGFQQLKAKLPPVSRRGL
                     ILIDPPYEMKTDYQAVVSGIAEGYKRFATGTYALWYPVVLRQQIKRMIHDLEATGIRK
                     ILQIELAVLPDSDRRGMTASGMIVINPPWKLEQQMNNVLPWLHSKLVPAGTGHATVSW
                     IVPE"
     gene            7098..9140
                     /gene="prlC"
                     /locus_tag="ACHOSC_RS13200"
                     /old_locus_tag="pgaptmp_002640"
     CDS             7098..9140
                     /gene="prlC"
                     /locus_tag="ACHOSC_RS13200"
                     /old_locus_tag="pgaptmp_002640"
                     /EC_number="3.4.24.70"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020317218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptidase A"
                     /protein_id="HGU4548555.1"
                     /translation="MTNPLLTPFELPPFSKILPEHVVPAVTKALNDCRENVERVVAQG
                     APYTWENLCQPLAEVDDVLGRIFSPVSHLNSVKNSPELREAYEQTLPLLSEYSTWVGQ
                     HEGLYKAYRDLRDGDHYATLNTAQKKAVDNALRDFELSGIGLPIEKQQRYGEIATRLS
                     ELGNQYSNNVLDATMGWTKLVTDEAELAGMPESALAAAKAQAEAKELEGYLLTLDIPS
                     YLPVMTYCDNQALREEMYRAYSTRASDQGPNAGKWDNSKVMEEILALRHELAQLLGFE
                     NYAFKSLATKMAENPQQVLDFLTDLAKRARPQGEKELAQLRAFAKAEFGVDELQPWDI
                     AYYSEKQKQHLYSISDEQLRPYFPENKAVNGLFEVVKRIYGITAKERKDVDVWHPDVR
                     FFELYDENNELRGSFYLDLYARENKRGGAWMDDCVGQMRKADGSLQKPVAYLTCNFNR
                     PVNGKPALFTHEEVITLFHEFGHGLHHMLTRIETAGVSGISGVPWDAVELPSQFMENW
                     CWEPEALAFISGHYETGEPLPKELLDKMLAAKNYQAALFILRQLEFGLFDFRLHAEFR
                     PDQGAKILETLAEIKKLVAVVPSPSWGRFPHAFSHIFAGGYAAGYYSYLWADVLAADA
                     FSRFEEEGIFNRETGQSFLDNILSRGGSEEPMDLFKRFRGREPQLDAMLEHYGIKG"
     gene            9148..9900
                     /gene="rsmJ"
                     /locus_tag="ACHOSC_RS13205"
                     /old_locus_tag="pgaptmp_002641"
     CDS             9148..9900
                     /gene="rsmJ"
                     /locus_tag="ACHOSC_RS13205"
                     /old_locus_tag="pgaptmp_002641"
                     /EC_number="2.1.1.242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709277.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008990 - rRNA
                     (guanine-N2-)-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(1516)-N(2))-methyltransferase
                     RsmJ"
                     /protein_id="HGU4548556.1"
                     /translation="MKICLIDETGAGDGALSVLAARWGLEHDEDNLMALVLTPEHLEL
                     RKRDEPKLGGIFVDFVGGAMAHRRKFGGGRGEAVAKAVGIKGDYLPDVVDATAGLGRD
                     AFVLASVGCRVRMLERNPVVAALLDDGLARGYADAEIGGWLQERLQLIHASSLTALTD
                     ITPRPQVVYLDPMFPHKQKSALVKKEMRVFQSLVGPDLDADGLLEPARLLATKRVVVK
                     RPDYAPPLANVATPNAVVTKGHRFDIYAGTPV"
     gene            complement(9949..11418)
                     /gene="dtpB"
                     /locus_tag="ACHOSC_RS13210"
                     /old_locus_tag="pgaptmp_002642"
     CDS             complement(9949..11418)
                     /gene="dtpB"
                     /locus_tag="ACHOSC_RS13210"
                     /old_locus_tag="pgaptmp_002642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide/tripeptide permease DtpB"
                     /protein_id="HGU4548557.1"
                     /translation="MNTTTPMGMLQQPRPFFMIFFVELWERFGYYGVQGVLAVFFVKQ
                     LGFSQEQAFVTFGAFAALVYGLISIGGYVGDHLLGTKRTIVLGALVLAIGYFMTGMSL
                     LKPDLIFIALGTIAVGNGLFKANPASLLSKCYPPKDPRLDGAFTLFYMSINIGSLIAL
                     SLAPVIADRFGYSVTYNLCGAGLIIALLVYIACRGMVKDIGSEPDFKPMSFSKLLYVL
                     LGSVVMIFVCAWLMHNVEVANLVLIVLSIVVTIIFFRQAFKLDKTGRNKMFVAFVLML
                     EAVVFYILYAQMPTSLNFFAINNVHHEILGFSINPVSFQALNPFWVVLASPILAGIYT
                     HLGNKGKDLSMPMKFTLGMFMCSLGFLTAAAAGMWFADAQGLTSPWFIVLVYLFQSLG
                     ELFISALGLAMIAALVPQHLMGFILGMWFLTQAAAFLLGGYVATFTAVPDNITDPLET
                     LPVYTNVFGKIGLVTLGVAVVMLLMVPWLKRMIATPESH"
     gene            11584..11637
                     /locus_tag="ACHOSC_RS13215"
                     /old_locus_tag="pgaptmp_002643"
     CDS             11584..11637
                     /locus_tag="ACHOSC_RS13215"
                     /old_locus_tag="pgaptmp_002643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4548558.1"
                     /translation="MVLNVVLAGDFDCVRRS"
     gene            complement(11736..12170)
                     /gene="uspA"
                     /locus_tag="ACHOSC_RS13220"
                     /old_locus_tag="pgaptmp_002644"
     CDS             complement(11736..12170)
                     /gene="uspA"
                     /locus_tag="ACHOSC_RS13220"
                     /old_locus_tag="pgaptmp_002644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein UspA"
                     /protein_id="HGU4548559.1"
                     /translation="MAYKHILIAVDLSPESKVLVEKAVSMARPYNAKVSLIHVDVNYS
                     DLYTGLIDVNLGDMQKRISEETHHALTELSTNAGYPITETLSGSGDLGQVLVDAIKKY
                     DMDLVVCGHHQDFWSKLMSSARQLINTVHVDMLIVPLRDEEE"
     gene            12561..12896
                     /gene="uspB"
                     /locus_tag="ACHOSC_RS13225"
                     /old_locus_tag="pgaptmp_002645"
     CDS             12561..12896
                     /gene="uspB"
                     /locus_tag="ACHOSC_RS13225"
                     /old_locus_tag="pgaptmp_002645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein UspB"
                     /protein_id="HGU4548560.1"
                     /translation="MISTVALFWALCVVCIVNMARYFSSLRALLVVLRNCDPLLYQYV
                     DGGGFFTSHGQPNKQVRLVWYIYAQRYRDHHDDEFIRRCERVRRQFILTSALCGLVVV
                     SLIALMIWH"
     gene            complement(13141..14640)
                     /gene="pitA"
                     /locus_tag="ACHOSC_RS13230"
                     /old_locus_tag="pgaptmp_002646"
     CDS             complement(13141..14640)
                     /gene="pitA"
                     /locus_tag="ACHOSC_RS13230"
                     /old_locus_tag="pgaptmp_002646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006817 - phosphate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic phosphate transporter PitA"
                     /protein_id="HGU4548561.1"
                     /translation="MLHLFAGLDLHTGLLLLLALAFVLFYEAINGFHDTANAVATVIY
                     TRAMRSQLAVVMAAVFNFLGVLLGGLSVAYAIVHMLPTDLLLNMGSSHGLAMVFSMLL
                     AAIIWNLGTWYFGLPASSSHTLIGAIIGIGLTNALMTGTSVVDALNIPKVLSIFGSLI
                     VSPIVGLVFAGGLIFLLRRYWSGTKKRARIHLTPAEREKKDGKKKPPFWTRIALILSA
                     IGVAFSHGANDGQKGIGLVMLVLIGVAPAGFVVNMNATGYEITRTRDAINNVEAYFEQ
                     HPALLKQATGADQLVPAPEAGATQPAEFHCHPSNTINALNRLKGMLTTDVESYDKLSL
                     DQRSQMRRIMLCVSDTIDKVVKMPGVSADDQRLLKKLKSDMLSTIEYAPVWIIMAVAL
                     ALGIGTMIGWRRVATTIGEKIGKKGMTYAQGMSAQMTAAVSIGLASYTGMPVSTTHVL
                     SSSVAGTMVVDGGGLQRKTVTSILMAWVFTLPAAVLLSGGLYWLSLQFL"
BASE COUNT         3774 a         3787 c         3816 g         3371 t
ORIGIN      
        1 ggattatcct gtctatgatt ttctacttag aaggatatgg cactaactat gcatcttata
       61 gcttactgct ggcatctatt taaaatagca attgagtcaa atagcccatc aagatagcca
      121 tacctaatat aaccgtcagg aatgcagcta tcatcggcat tctgaacatt gatttaagta
      181 atatcacttc cgtcaggctt gcgccggcac tgccgatgat taatgccatt aatgctccca
      241 gacccattcc ttttgtcatc agaacagatg ccagagggat aactgcctca gcccggatat
      301 acagcggaat accgacaacg gcgcttaatg ggatggcgaa gggattatct gctcctgcat
      361 gagcggcaat ccacgctgag ggaataaagc cataaataaa agatcctatt aatactccta
      421 acaaaagata tggcagtaca tctttaaact gttgcaacgc atctttggtg gctagtttta
      481 tgggattaat acttactaca gctttggtac tgcaacaggt aactacgggg ttctcaatgg
      541 ctttagccgc tggactacaa caactcactt ttatgggcgt atatgtcgtt cccggcgaag
      601 tttttgctgg agcacaacaa tttgctgatg agcttttact ggcaatgata tgacgttcaa
      661 accccagaga atccaggata atactggcaa gaacggagac gccggctgcg ataatcgcgt
      721 acaacaaggt gactttccaa ccaaaggtca tccacattag tccgacgata atgggattaa
      781 gtaatggtga aacaaataaa aaagtgagag tcggaccaaa cccggctttc gctgacaaca
      841 atccacgtaa catgggtatt gtcgagcaac tacagaacgg ggtaacggct cccaacagag
      901 cagccaggag atagcctctc ccttttctgg cccccatcat ctgctggatt ttatggtctg
      961 gcaccttttg tcttatcagg ctgacaccgg cactaatcac tataaacagc aacgaaagct
     1021 cgacggcgag aaacacaaac atctctgcgg catcttgcag catcgcaagc caactactca
     1081 tacgtaacct cattggataa atctatattt ccagaataat cgaaatgaaa ttcagatcaa
     1141 gatatttctg gtattatcga aatatcaacc agacagaggg gtgttatatg cagttggaag
     1201 aagtcgctaa agcgctgaaa gagctaggtc acccgacccg tttgttcata tttaaacatc
     1261 ttgtcaaagc cggggaacaa gggttgccgg tcggtgagtt gcaaaagcag cttgggatac
     1321 ctggctccac actcagccac catatatcag cactggtatc ggtaggactg gtcaaacaaa
     1381 accgtgagag tcggacgctg atgtgtgttt cgcaatacgc aatgctggaa gcgataattg
     1441 aattcttacg cgaggagtgc tgcgtaaata gcaaaattgc ctgatgcgct acgctctaca
     1501 tatttcatgc catatattga atttgcacgg ttttgtaggt cggataagac gcgttagcgt
     1561 cgcatccgac ataaacaaag cgcactttac caactatcgg cagtggatat cgccgcttta
     1621 tttcaggcgc ttacccgctt catcaaccac tttctcgcca tcttccttgg agaatgcgcc
     1681 tttttgcgca tctggcagaa tttccagcac cacttctgaa gggcggcaca ggcgagttcc
     1741 cagcggcgtc accacaatcg ggcgattaat cagaatcggg tgctgaagca taaagtcgat
     1801 taaccgatcg tcagtaaatt tatcttccgc aaggcccagc tcctcatacg gttcgacgtt
     1861 tttacgcagc agcgcgcgta cggaaatccc catatcggca atgagtttga ccagttcatc
     1921 gcgcgttggc ggagtttcca gataatggat aatggtcggt tctgtgccgc tgttgcggat
     1981 catctccagc gtattacgcg acgtgccgca ggccgggttg tgataaatgg taatgttgct
     2041 catatcagta tctcattaca aagtgaaaga gagacgtagc gccagcgcag ccagcgtcac
     2101 aaacagcaca ggcagggtca tgataatccc tgtacggaag taatatcccc agctgatagt
     2161 catattcttc tgcgaaagta cgtgcagcca gagtagcgta gccaggctac caattggggt
     2221 aattttcggt cccaaatcgc agccaatcac attggcataa accatcgctt ctttgataac
     2281 gccagatgcc gtgctgccat caatggacaa cgcgccaacc agtaccgtcg gcatattgtt
     2341 cataatagaa gagaggaagg cggtgaggaa tccggtgccg agcgtcgcgg cccacaggcc
     2401 gttatccgcc agcacgttga gtacgccaga aagatattcc gttaatccgg cattgcgcag
     2461 gccataaacc accagataca tgccgagcga gaagatgaca atctgccagg gggcaccgcg
     2521 caggacttta cccgtattaa tcgcatgacc gcgtttagcg acgacaaata atatcagcgc
     2581 gcccacagct gcaatggcgc tcaccggaat gccgagcggt tccaggacga aaaatcccac
     2641 cagcagaagc agtaaaacaa cccagccagt tttgaacgta gcaggatctt tgatcgcttc
     2701 tgcgggagat ttcagcagcg ccatatcgta gttctgcgga atatctttgc gaaaatagag
     2761 atgtaacatc accagcgtgg caacaatcgc ggcgatatcc accggcacca tcaccgaggc
     2821 gtattcgcga aagccgaggc caaagaaatc agcggaaacg atattcacca ggttggagac
     2881 aataagcggc aggctggcgg tatcggcaat gaatccggcc gccatcacga acgccagcgt
     2941 agtgccttta ctgaacccta aagccagcag catggcgatg acaatcggtg tcaaaataag
     3001 cgccgcgcca tcattggcaa acagggcggc aacggcagca ccgagcagga caatccaggt
     3061 aaacagcaag cgaccacgac cattacccca gcgtgagacg tgcagcgccg cccattcaaa
     3121 aaagccggac tcatccagca gcaggctgat gataatgacg gcgataaacg cagccgtcgc
     3181 gttccagacg atattccaca ccaccggaat atcacccgga tggaccacgc ccgtaactaa
     3241 cgccagtact gcgccgagcg ttgcactcca gccgatgcct aaacctttcg gctgccagat
     3301 aaccaatacg atggtcagga caaagatagc gcctgccagt aacataatgc ctcccggata
     3361 aaacacatct gaaaattcat atgtgtttag ctaaattttt aactgcaaat gttcttactg
     3421 tccccggaac agttttgtcg agccaggttg cggacaatcg cctgaacctt ttcctgttca
     3481 catcgccagg cctcatcaat aattttcgcc gcccatgctg gaatatgcgg tgataagcgg
     3541 taatgaaccc acttaccttg cttgcggtcc agcaatagcc cgctttcacg cagcaatgcc
     3601 aggtggcggg agatcttggg ctgcgactgg tcgagagcag tgcagagatc gcagacgcat
     3661 aactctccca gttcgctgag cagtaaaacg atgcccagac gggtttcatc agcaagaatt
     3721 ttgaacaatt ggatgggtaa cagaaatgac atattgcgct cctgattgtt gcaggtagtg
     3781 tctctcttcg aagcggataa gtcaaaaaca tatatgactt aacgaatgtg taagtgcaga
     3841 ggaaggtaat aggtgtgaat tttgagttgg ctattcattt gaaaggaggt ctgaatcagg
     3901 acgtaaaaaa accgactttg cgtcggtttt ttactttcca gccctgagtt ggtggctctg
     3961 gctggagtga gagagttctt atctaacagc tcaatactat tagccagtgg ctaacattgc
     4021 aagaattagc gatttcttca gctggcggag ggggcacttc ggtatatatc gcaatgacga
     4081 tttatatact ttcactgggt catcgtcata ttaagccttg tccgaatccg gatgtaaaaa
     4141 aaccgacttt gcgtcggttt tttactttcc agccctgagt tggtggcact ggctggagtg
     4201 agaatgccat tatctaacgc atcaatgcta ataccatact gaaactattg caaggacatg
     4261 ctggttttat aacctgaatg tactgtatga ttatccagtt agctctgagg cattttcact
     4321 ctggtaatgc gcataaacgc tttcaaagtc ctggtcagaa gtacgggtgg tgccgttaac
     4381 tgatgctctg gccggagtga gaatgccatt atctaacaat agggcatgcg ccgtgacagg
     4441 cagtggatga gtaagcggat gcattctcac tccatcgcat ggagaaaacg ggtgattgat
     4501 aaagcaatca tcgttcttgg ggcgttaatt gcgctgctgg aactgatccg ctttctgctt
     4561 cagcttctga actgatagcg gaaacgtaat taagggctaa gagcacacta ctcttagccc
     4621 tttaacattt aacgcattgt cacgaactct tctgccgccg ttgggtgaat ggcgacggta
     4681 ttgtcgaagt cttttttggt tgcacccatc ttcagcgcca ccgcgaagcc ctgcaacatt
     4741 tcgtccatac caaagccaat gccgtgaata ccgacaatct tctcttccga tccaacgcac
     4801 accagcttca tgcggcacgg ctggcggtga gtggtgacgg cggtatacat cgcggtgaaa
     4861 gaggatttat acactttcac ctgatcgtcg ccatactgct cgcgcgcctg cggttccgtt
     4921 aaaccaacag taccaatcgg cggatggctg aagaccacgg tcggaatgtt gctgtaatcc
     4981 agatgctcat ccggcttgtt attaaacagg cgttcagaga gacggcgacc cgctgcaact
     5041 gccaccggtg tcagctccac tgcacccgtg ttatcgccca ccgcgtaaat accttcaata
     5101 ttggtgtttt gatatttatc gacgacgata tagccttttt cgttagtttt aacgccagcg
     5161 gcttccaggt tgatgttgtc attggcaggc tcgcgaccaa tcgcccaaat caggcaatcc
     5221 accgtttcac tgcgaccatc ttccagctcc agcgtcaggc taccatcggc atttttcact
     5281 accgctttcg ggatggcgtt ggtgtgcagc tgcgggcctt cggcgttcat cacttcgacc
     5341 agcgtttcgg aaatcatcgg gtcgaagctg cgcagcggcg catgtttacg cacaaacaga
     5401 tgcgttttcg cgccgaggcc gttaatcacg cccgccagct caacggcgat gtaacccgcg
     5461 ccaacaaccg ccacgcgctc tggcaaagca ggaagggcga agaagccatc agaatcaata
     5521 ccgtattcca cgcccggaat atccgggtgg ctcggacgac cgcctgtggc gatcagaata
     5581 tgatcggccg tgatggtttc gccgtttacc tccagcgttt tggcatcaac gaagcgggca
     5641 aagcctttga ttacatcaac gttattttta ccgagcacgt tttcatagga agtatgaata
     5701 cggtcgatat aggcggtacg gctggcgatc aacgtttccc agttgaattt attgatagtg
     5761 gtatcaaaac cataatccgg gccgtacata tggatcgctt cacggatttg cgccgcgtgc
     5821 cacatcactt ttttcggcac acagccaaca tttacgcagg tgccgcccag ctctttggct
     5881 tcaatcagcg cacatttctg gccgtacata gccgcgcggt tgatggaggc gataccgccg
     5941 ctgccgccgc cgatggcgat gtaatcatag tgtttagtca tgacaaagtg tccttatcgt
     6001 tgattaccgc gattgtagcg cgagacgtaa taggtgccag caatggctgc aattactccg
     6061 gcacgatcca gcttacggtg gcgtgcccgg tgcctgccgg aaccagtttg ctgtgcagcc
     6121 acggcagcac gttattcatc tgctgttcca gtttccacgg cgggttaatc acaatcatgc
     6181 cggaagcggt catgccacgg cgatcgctgt ctggcagtac cgccagttca atttgcagaa
     6241 ttttgcgaat accggtcgct tccagatcgt ggatcatgcg cttaatttgc tgacgcagca
     6301 ccaccggata ccacagtgcg taagtaccag tggcgaaacg tttgtaacct tctgctatcc
     6361 cgctgaccac cgcctgatag tcggttttca tttcatacgg cgggtcgata aggattaaac
     6421 cacggcggga aaccggcggc agtttggcct taagctgctg gaaaccatcg gctttttcga
     6481 cgcgcgcacg gctgtctttc tgaaattcag aacgcagcaa cgggtaatcg ctcgggtgca
     6541 gttcggtcag ttgcaggctg tcttgttcgc gcagtagctg gcgagcaatc aatggcgaac
     6601 cggggtagta acgcaactga ccgctacggt tgaagtgttt taccacattg atgtacgcct
     6661 ccagttctgc gggcaaatcg tcctgctgcc agatacgggc gatgccttcg agatattcgc
     6721 cggtacgctc ggcatgttcg ctgcctaact gataacgccc ggcccctgcg tgggtgtcga
     6781 gatagagaaa cggtttatct ttctctttca gcgactcgat gatcaggctc tgaacggtat
     6841 gtttaaggac gtcggcgtgg ttgccagcgt gaaagctgtg gcgataactg agcatgggta
     6901 aaggtgttcc ggtaagtaaa aatcggcccg tattcggggc gcacaaaagc gtatcaggac
     6961 agtataccga aaagaggccg ccgccgcgaa agcgtaacgt ttctcattga aattcactac
     7021 acttaacccc atgctacaca cattatgtaa agcgcctgtt gagcgcttcc ttaacctctt
     7081 taaccaggac tgcgcgaatg acgaatccgt tactgactcc ctttgaattg cctccgtttt
     7141 ctaaaattct cccggaacat gtcgttccag ccgtgactaa ggcgctgaac gactgccgcg
     7201 aaaatgtgga gcgcgtagta gcgcaaggag caccgtacac ctgggaaaat ctctgccagc
     7261 cgttggcgga agtggacgat gtgctggggc gtatcttctc cccggtcagc cacctgaact
     7321 cggtgaaaaa tagcccggaa ctgcgtgaag cgtacgaaca aaccctgccg ctgctgtcgg
     7381 aatacagcac ctgggtaggg caacatgaag ggctgtataa agcgtatcgc gacctgcgcg
     7441 atggcgatca ttacgccacg ctgaacacgg ctcagaaaaa agcggttgat aacgcactgc
     7501 gcgacttcga actctctggc atcggtctgc caatagagaa acaacaacgc tacggtgaga
     7561 ttgccactcg tctttccgag ctgggcaacc agtacagcaa caacgtcctc gatgcgacga
     7621 tgggctggac caaactcgtt accgacgaag cggagctggc ggggatgcct gaaagcgcgc
     7681 tggctgcggc aaaagcccag gccgaagcga aagagctgga aggctacctg ctgacgctgg
     7741 atatcccaag ctacctgccg gtaatgacct actgcgacaa ccaggccttg cgtgaagaga
     7801 tgtatcgcgc ttacagcacc cgcgcctccg atcaaggccc gaacgccggt aaatgggata
     7861 acagcaaggt gatggaagag atcctcgctc tgcgtcacga actggcgcaa ctgctgggct
     7921 ttgaaaacta cgcctttaaa tcccttgcca ctaaaatggc agaaaacccg cagcaggtgc
     7981 tggatttctt aaccgatctg gcaaaacgcg cgcgtccgca aggcgaaaaa gagctggcac
     8041 aactgcgcgc cttcgccaaa gcggaatttg gcgtcgatga gttgcagccg tgggatatcg
     8101 cgtactacag cgaaaaacaa aaacagcacc tctacagcat cagcgacgag cagctgcgtc
     8161 cgtacttccc agaaaacaaa gcggttaacg gcctgtttga agtggtgaaa cgtatttacg
     8221 gcatcaccgc taaagagcgt aaagatgttg atgtctggca tccggatgta cgtttcttcg
     8281 aactgtatga cgaaaataac gaactgcgcg gcagcttcta cctcgatctg tatgcccgtg
     8341 aaaacaagcg cggcggggcg tggatggatg actgcgtagg ccagatgcgt aaagctgatg
     8401 gttcgctgca aaaaccggtc gcgtatttga cttgtaactt caaccgcccg gtaaatggta
     8461 aaccggcgct gttcactcac gaggaagtga tcaccctgtt ccacgagttc ggtcacggcc
     8521 tgcaccatat gctgacccgc atcgaaaccg ctggtgtttc cggtatcagc ggtgtgccgt
     8581 gggatgcggt cgaactgccg agtcagttta tggaaaactg gtgctgggag ccggaggcgc
     8641 tggcgtttat ctccggtcac tatgaaaccg gcgaaccgct gccgaaagag ttgctggata
     8701 aaatgctggc ggcgaagaac taccaggcgg cgctgtttat tctgcgtcag ctggagttcg
     8761 gcctgtttga tttccgcctt catgccgagt tccgcccgga tcagggggca aaaatcctcg
     8821 aaactctggc agaaatcaag aaactggttg ccgtggtgcc atctccgtcc tggggccgtt
     8881 tcccgcacgc tttcagccat attttcgccg gtggttatgc ggcaggttac tacagctacc
     8941 tgtgggctga cgtactggcg gcagatgctt tctcgcgctt tgaggaagag ggcattttca
     9001 accgtgaaac cgggcagtcg ttcctcgaca acattctgag ccgtggcggt tcagaagagc
     9061 cgatggatct gttcaaacgc ttccgtggtc gtgaaccgca gctggatgcg atgctggagc
     9121 attacggcat taagggctga tcattcagtg aaaatctgct taattgatga aacaggcgcc
     9181 ggagacggcg ccttatctgt tctggcggcc cgctgggggc tggagcacga tgaagacaac
     9241 ctgatggcgc tggtgttaac gccggaacat ctggaattgc gcaagcgtga tgagccaaaa
     9301 cttggcggca tatttgttga ttttgttggc ggagcgatgg cgcaccgacg caaattcggc
     9361 ggtggtcgcg gtgaggcggt ggcgaaagcg gtgggcatta aaggcgatta tttgccggat
     9421 gtggtggacg ccaccgcagg gctggggcgc gatgcctttg tgctggcttc agtcggttgc
     9481 cgcgtgcgga tgctggagcg taatccagtc gttgccgcat tgctcgacga cggcctggcg
     9541 cgtggttatg cggatgcgga aatcggcggc tggttgcagg agcgtttgca gttaattcac
     9601 gcctccagcc tgacggcgct aaccgatatt accccacgcc cgcaggtggt ctatctcgac
     9661 ccgatgttcc cgcataagca gaaaagcgcg ctggtgaaaa aagagatgcg tgtctttcag
     9721 tcgctggtgg ggccggatct tgatgccgat ggattgctgg agcctgcacg cttgctggcg
     9781 accaaacgcg tggtagtaaa gcgcccggac tacgcgccac cgctagcgaa tgtcgccacg
     9841 ccaaacgcgg ttgtgactaa agggcatcgc tttgatatct atgcaggtac gcccgtgtaa
     9901 aagtaaagca gggtagcgat aacgctaccc tgctttctgc aagaataatt aatggctttc
     9961 cggcgtcgca atcatgcgtt tcagccacgg caccatcaac agcatcacta ctgcaacgcc
    10021 cagcgtgacc agaccaatct taccaaacac gttggtatag acgggcaacg tctcaagcgg
    10081 atcggtaatg ttgtccggca ccgcggtaaa tgttgccaca tagccgccca gcaagaacgc
    10141 ggcagcctgc gtcaggaacc acatcccgag aataaagccc atcaaatgct gcggcaccag
    10201 ggcagcaatc atcgccaggc caagggcgct aataaacagt tcacctaagc tctggaataa
    10261 gtacaccagc acgataaacc atggcgatgt cagcccttgt gcatccgcaa accacattcc
    10321 cgcagctgcc gccgtcaaaa agcccagtga gcacataaac atgccgagag taaatttcat
    10381 cggcatcgag aggtctttgc ctttgttacc cagatgcgtg taaatgcctg ccagtattgg
    10441 gctggcgagt accacccaga acgggttaag cgcctggaag ctgaccgggt tgatggaaaa
    10501 accgagaatt tcatgatgca cgttgttgat ggcaaagaag ttcagcgatg ttggcatctg
    10561 ggcgtagaga atgtaaaaca ccaccgcttc gagcatcagg acaaaggcga caaacatttt
    10621 attgcgcccg gttttatcca gcttgaatgc ctgacgaaag aagatgatgg tgacgacgat
    10681 ggagagaaca atcagcacca gattggcgac ttctacgttg tgcatcagcc atgcacatac
    10741 gaagatcatc accacgctgc caagtaatac gtacaacagt ttgctgaagc tcatcggctt
    10801 gaagtcgggt tcagaaccaa tgtctttcac cattccacga caggcgatgt aaaccagtaa
    10861 tgcgataatt aaccccgccc cgcacaggtt gtaggtgact gaataaccga atctatcagc
    10921 gatcacaggg gccagcgata acgctatcaa cgagccgata ttgatcgaca tatagaacag
    10981 ggtgaatgcg ccatcaagcc gcggatcttt cggcggatag cacttcgaaa gcaagctggc
    11041 tgggttagct ttaaacaggc cgttaccgac agcgatagtc cccagggcga tgaaaatcag
    11101 gtcaggctta agtagcgaca tgccggtcat gaagtagcca atcgccagca caagtgctcc
    11161 gagaacaatg gtgcgtttgg tccccagcag gtggtcgccg acatagccgc caatggaaat
    11221 gaggccatag accagcgcag caaaagcacc aaaagtgaca aaagcctgct cttgcgagaa
    11281 tccaagctgt ttaacgaaga aaaccgccag tacgccctgc acgccgtagt agccgaatcg
    11341 ctcccataac tcgacaaaaa agatcatgaa aaatgggcga ggttgctgca gcatccccat
    11401 gggtgttgtt gtattcatat ttattaacct tccaatacca tcctgaaaag tatgaacgcg
    11461 taagggataa cgcgcgaaaa agtttacatc tgtcagagat tcttaatatt gatgcggtga
    11521 atagattaat acactattgc aacagaaaat aaccagctga tcacactaat gatggaaaat
    11581 atgatggtgt taaatgttgt attggcagga gattttgatt gcgtacgcag atcgtaggcc
    11641 agataagggg tttacgctgc atcaggtaac aaggcgcgcc aaaaagcaaa acgcccgcat
    11701 atgcgggcgt tcaggaacac gtcgtggagg gatgattatt cttcttcgtc gcgcagcgga
    11761 acaatcagca tatcaacgtg aacggtgttg atcagctgac gtgcggaaga catcagtttg
    11821 ctccagaagt cctggtggtg accacaaacc accagatcca tatcgtattt cttgattgca
    11881 tcgaccagaa cctggcccag gtcgccgctg ccgctcaggg tttcagtgat tgggtagcct
    11941 gcattagtgg aaagctcggt cagcgcatga tgtgtctctt cagagatgcg tttctgcata
    12001 tcacccagat tcacatcaat aagcccggtg tataggtcag agtagtttac atctacgtgg
    12061 atcagagaaa ctttcgcatt gtaggggcga gccatagaga ctgctttctc taccagaact
    12121 ttgctttccg gggagagatc gactgcgatg agaatgtgtt tataagccat agtgttactc
    12181 cttccataaa gttgtcgatg actggccagc tagcgtttct tgtgccgtta cgtcacccgc
    12241 gtcctgcgaa ctacgtgcgc ggggctaacc cagccgtaaa gattcagtgt taagaccatc
    12301 cttaccttat agcgacccgg atgatccgtc aatccgcctt gcttacccgt tagataaaca
    12361 aattttcgac atgaatatat tgatagtggt taaccttctg gaaaaaaaac aacctgatct
    12421 cctacactat ctatagagcc gctcggatgt tggtcaccac aaaagcggtt tctccaggac
    12481 ctggtgttaa ctgattggca gcgtatcggg agcggtagcc ccggggtgga actccgcggg
    12541 caggtcgccg gggaggaggt atgataagca ccgtcgcatt attttgggct ttatgtgtcg
    12601 tttgcattgt taacatggcg cgctatttct catcactacg cgcgttgtta gtggtactgc
    12661 gtaactgcga tccattgctc tatcaatatg ttgatggagg gggctttttt acctcacatg
    12721 gccaacccaa caaacaggtg cgtctcgttt ggtatatcta tgcccaacgt tatcgcgatc
    12781 atcacgatga tgaatttatt cgccgctgtg agcgggtgcg tcggcagttt attctgacca
    12841 gcgcattgtg tggtctggtg gtggtcagcc tgattgcatt gatgatttgg cattaagcac
    12901 ttcagcaata ataacgcata aaaaaagcgg gccagtaaac tgacccgctc gagactgatt
    12961 acaaacgcaa aattgcctga tgcgctacgc ttatcaggat ctacataatc tctgcaatct
    13021 attgaatttg catgtttttg taggccggat aaggcgttca cgccgcatcc ggcatgaaca
    13081 aagcgcacta tgtcacaatc tgaagcgggc cagctgaccc gctcgttttg gtgtgtacga
    13141 ttacaggaac tgcaaggaga gccagtacag tccgccggaa agcagtaccg cagccggaag
    13201 ggtaaacacc caggccatca gaatactggt tacggtttta cgctgtaagc cgccgccatc
    13261 taccaccatc gtccccgcga cagaagagga gagtacgtga gtagtggaaa ccggcatccc
    13321 ggtataactc gccaggccga tagacactgc cgccgtcatc tgggccgaca tcccctgagc
    13381 gtaggtcatg cctttcttac cgattttctc accgatagtc gttgccacac ggcgccagcc
    13441 aatcatcgta ccgataccta acgccagcgc gaccgccatg atgatccaca ccggtgcata
    13501 ctcgatggtg ctaagcatgt cggacttcag tttcttcaac aggcgctgat cgtcagcact
    13561 cacaccaggc atcttcacca ctttgtcgat agtgtcagaa acgcacagca taatgcggcg
    13621 catctggcta cgttgatcaa gcgacagctt gtcgtagctt tccacatcgg tagtcaacat
    13681 gcctttcagg cggttgagcg cgttaatggt attcgacgga tggcaatgga actccgcggg
    13741 ttgcgttgcg ccagcttccg gagccggtac taactgatca gcaccggtag cctgtttgag
    13801 cagcgcagga tgctgctcaa agtaagcttc gacgttgttg atggcatcac gggtacgggt
    13861 gatttcgtag ccagtggcat tcatgttcac cacgaagcct gctggtgcga cgccaatcaa
    13921 taccaacata accagaccaa tgcctttctg accatcgttc gcgccgtgcg aaaacgccac
    13981 gccgatagcg gaaaggatca gcgcaatacg cgtccagaac ggcggctttt tcttgccgtc
    14041 tttcttttca cgctccgctg gggtcaggtg gatacgggcg cgtttcttgg tgccgctcca
    14101 gtagcgacgc agcaagaaaa tcagaccgcc agcaaacacc aggccgacaa taggggaaac
    14161 gatcagagaa ccgaaaatac ttaatacttt cgggatattg agtgcatcca ccactgacgt
    14221 cccggtcatc aacgcattgg ttaaaccaat cccgatgatc gcgccaatca gcgtatgaga
    14281 gctggatgca ggtaaaccaa agtaccaggt acccaggttc cagataatcg ccgccagcaa
    14341 catagagaac accatggcaa ggccatgaga cgatcccata ttaagcagca gatccgtcgg
    14401 cagcatatgc acaatggcat aggcaacact cagaccaccc agcaaaacac ccaaaaagtt
    14461 gaacaccgct gccataacca cggcgagctg agaacgcatc gcgcgggtat agataacggt
    14521 tgccacggcg ttggctgtgt catggaaacc attgatggct tcgtagaaca gcacaaaagc
    14581 cagtgcaagc aataataaca gcccggtatg caaatccagg ccagcaaaca aatgtagcat
    14641 aggacgttac gccattttga ggacatgaac gcggcgcatt atcagtgact ttcgcggcgc
    14701 gggcaaagtg aaatatagac tttttttgat ttgcctcctg tatggatt
//
LOCUS       DAZKHF020000109        14519 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2201S31241
            SAMN43267800-rid25106413.denovo.110, whole genome shotgun sequence.
ACCESSION   DAZKHF020000109 DAZKHF020000000
VERSION     DAZKHF020000109.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267800
            Sequence Read Archive: SRR30307263
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 14519)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14519)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 14519)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350581.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350581.2
            Genome Coverage       :: 151x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:21:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,652
            CDSs (total)                      :: 5,557
            Genes (coding)                    :: 5,320
            CDSs (with protein)               :: 5,320
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 237
            CDSs (without protein)            :: 237
            Pseudo Genes (ambiguous residues) :: 0 of 237
            Pseudo Genes (frameshifted)       :: 102 of 237
            Pseudo Genes (incomplete)         :: 130 of 237
            Pseudo Genes (internal stop)      :: 52 of 237
            Pseudo Genes (multiple problems)  :: 41 of 237
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14519
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267800-rid25106413.denovo.110"
                     /serotype="O 26:H11"
                     /isolate="2201S31241"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-01"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            168..839
                     /locus_tag="ACHOSC_RS13235"
                     /old_locus_tag="pgaptmp_002647"
     CDS             168..839
                     /locus_tag="ACHOSC_RS13235"
                     /old_locus_tag="pgaptmp_002647"
                     /EC_number="3.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016241930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIG-L deacetylase family protein"
                     /protein_id="HGU4548562.1"
                     /translation="MDKVLDSALPSSANKRKGILAIGAHPDDIELGCGASLARLAQKG
                     IYIAAVVMTTGNSGTDGIIDRHEESRNALKILGCHQTIHLNFADTRAHLQLNDMISAL
                     EDIIKNQIPSDVEIMRVYTMHDADRHQDHLAVYQASMVACRTIPQILGYETPSTWLSF
                     MPQVFESVKEEYFTVKLAALKKHKSQERRDYMRHDRLRAVAQFRGQQVNSDLGEGFVI
                     HKMIL"
     gene            849..2045
                     /gene="yaiP"
                     /locus_tag="ACHOSC_RS13240"
                     /old_locus_tag="pgaptmp_002648"
     CDS             849..2045
                     /gene="yaiP"
                     /locus_tag="ACHOSC_RS13240"
                     /old_locus_tag="pgaptmp_002648"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HGU4548563.1"
                     /translation="MKTWIFICMSIAMLLWFLSTLRRKPSQKKGCIDAIIPAYNEGPC
                     LAQSLDNLLRNPYFCRVICVNDGSTDNTEAVMAEVKRKWGDRFVAVTQKNTGKGGALM
                     NGLNYATCDQVFLSDADTYVPPDQDGMGYMLAEIERGADAVGGIPSTALKGAGLLPHI
                     RATVKLPMIVMKRTLQQLLGGAPFIISGACGMFRTDVLRKFGFSDRTKVEDLDLTWTL
                     VANGYRIRQANRCIVYPQECNSPREEWRRWRRWIVGYAVCMRLHKRLLFSRFGIFSIF
                     PMLLVVLYGVGIYLTTWFNEFITTGPHGVVLAMFPLIWIGVVCVIGAFSAWFHRCWLL
                     VPLAPLSVVYVLLAYAIWIIYGLIAFFTGREPQRDKPTRYSALVEASTAYSQPSVTGT
                     EKLSEA"
     gene            2023..2604
                     /locus_tag="ACHOSC_RS13245"
                     /old_locus_tag="pgaptmp_002649"
     CDS             2023..2604
                     /locus_tag="ACHOSC_RS13245"
                     /old_locus_tag="pgaptmp_002649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001308433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyltransferase"
                     /protein_id="HGU4548564.1"
                     /translation="MKNYLKLNVKEKNIQIADQVIIDETAGEVVIGANTRICHGAVIQ
                     GPVVIGANCLIGNYAFIRPGTIISNGVKIGFATEIKNAVIEAEATIGPQCFIADSVVA
                     NQAYLGAQVRTSNHRLDEQPVSVRTPEGIIATGCDKLGCYIGQRSRLGVQVIILPGRI
                     ISPNTQLGPRVIVERNLPAGTYSLRQELIRTGD"
     gene            2606..3379
                     /gene="yaiO"
                     /locus_tag="ACHOSC_RS13250"
                     /old_locus_tag="pgaptmp_002650"
     CDS             2606..3379
                     /gene="yaiO"
                     /locus_tag="ACHOSC_RS13250"
                     /old_locus_tag="pgaptmp_002650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414892.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YaiO family outer membrane beta-barrel protein"
                     /protein_id="HGU4548565.1"
                     /translation="MIKRTLLAAAIFSALPAYAGLTSITAGYDFTDYSGDHGNRNLAY
                     AELVAKVENATLLFNLSQGRRDYETEHFNATRGQGAVWYKWNNWLTTRTGIAFADNTP
                     VFARQNFRQDINLALLPKTLFTTGYRYTKYYDDVEVDAWQGGVSLYTGPVITSYRYTH
                     YDSSDAGGSYSNMISVRLNDPRGTGYTQLWLSRGTGAYTYDWTPETRYGSMKSVSLQR
                     IQPLTEQLNLGLTAGKVWYDTPTDDYNGLQLAAHLTWKF"
     gene            3565..3840
                     /gene="frmR"
                     /locus_tag="ACHOSC_RS13255"
                     /old_locus_tag="pgaptmp_002651"
     CDS             3565..3840
                     /gene="frmR"
                     /locus_tag="ACHOSC_RS13255"
                     /old_locus_tag="pgaptmp_002651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414891.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formaldehyde-responsive transcriptional
                     repressor FrmR"
                     /protein_id="HGU4548566.1"
                     /translation="MPSTPEEKKKVLTRVRRIRGQIDALERSMEGDAECRAILQQIAA
                     VRGAANGLMAEVLESHIRETFDRNDCYSREVSQSVDDTIELVRAYLK"
     gene            3875..4984
                     /gene="frmA"
                     /locus_tag="ACHOSC_RS13260"
                     /old_locus_tag="pgaptmp_002652"
     CDS             3875..4984
                     /gene="frmA"
                     /locus_tag="ACHOSC_RS13260"
                     /old_locus_tag="pgaptmp_002652"
                     /EC_number="1.1.1.284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-(hydroxymethyl)glutathione dehydrogenase"
                     /protein_id="HGU4548567.1"
                     /translation="MKSRAAVAFAPGKPLEIVEIDVAPPKKGEVLIKVTHTGVCHTDA
                     FTLSGDDPEGVFPVVLGHEGAGVVVEVGEGVTSVKPGDHVIPLYTAECGECEFCRSGK
                     TNLCVAVRETQGKGLMPDGTTRFSYNGQPLYHYMGCSTFSEYTVVAEVSLAKINPEAN
                     HEHVCLLGCGVTTGIGAVHNTAKVQPGDSVAVFGLGAIGLAVVQGARQAKAGRIIAID
                     TNPKKFDLARRFGATDCINPNDYDKPIKDVLLDINKWGIDHTFECIGNVNVMRAALES
                     AHRGWGQSVIIGVAGSGQEISTRPFQLVTGRVWKGSAFGGVKGRSQLPGMVEDAMKGD
                     IDLEPFVTHTMSLDEINDAFDLMHEGKSIRTVIRY"
     gene            5077..5910
                     /gene="fghA"
                     /locus_tag="ACHOSC_RS13265"
                     /old_locus_tag="pgaptmp_002653"
     CDS             5077..5910
                     /gene="fghA"
                     /locus_tag="ACHOSC_RS13265"
                     /old_locus_tag="pgaptmp_002653"
                     /EC_number="3.1.2.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018738 - S-formylglutathione hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0046294 - formaldehyde catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S-formylglutathione hydrolase"
                     /protein_id="HGU4548568.1"
                     /translation="MELIEKHASFGGWQNVYRHYSQSLKCEMNVGVYLPPKAANEKLP
                     VLYWLSGLTCNEQNFITKSGMQRYAAEHNIIVVAPDTSPRGSHVADADRYDLGQGAGF
                     YLNATQAPWNEHYKMYDYIRNELPDLVMQHFPATTRKSISGHSMGGLGALVLALRNPD
                     EYVSVSAFSPIVSPSQVPWGQQAFAAYLGENKDAWLNYDPVSLISQGQRVAEIMVDQG
                     LSDDFYAEQLRTPNLEKICQEMNIKTLIRYHEGYDHSYYFVSSFIGEHIAYHANKLNM
                     R"
     gene            complement(6035..6574)
                     /gene="yaiL"
                     /locus_tag="ACHOSC_RS13270"
                     /old_locus_tag="pgaptmp_002654"
     CDS             complement(6035..6574)
                     /gene="yaiL"
                     /locus_tag="ACHOSC_RS13270"
                     /old_locus_tag="pgaptmp_002654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414888.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2058 domain-containing protein"
                     /protein_id="HGU4548569.1"
                     /translation="MAKLTLQEQLLKAGLVTSKKAAKVERTAKKSRVQAREARAAVEE
                     NKKAQLERDKQLSEQQKQAALAKEYKAQVKQLIEMNRITIANGDIGFNFTDGNLIKKI
                     FVDKLTQAQLINGRLAIARLLVDNNSEGEYAIIPASVADKIAQRDASSIVLHSALSAE
                     EQDEDDPYADFKVPDDLMW"
     gene            complement(6676..7887)
                     /gene="mhpT"
                     /locus_tag="ACHOSC_RS13275"
                     /old_locus_tag="pgaptmp_002655"
     CDS             complement(6676..7887)
                     /gene="mhpT"
                     /locus_tag="ACHOSC_RS13275"
                     /old_locus_tag="pgaptmp_002655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308435.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-(3-hydroxy-phenyl)propionate transporter MhpT"
                     /protein_id="HGU4548570.1"
                     /translation="MSTRTPSSSSSRLMLTIGLCFLVALMEGLDLQAAGIAAGGIAQA
                     FALDKMQMGWIFSAGILGLLPGALVGGMLADRYGRKRILIGSVALFGLFSLATAIAWD
                     FPSLVFARLMTGVGLGAALPNLIALTSEAAGPRFRGTAVSLMYCGVPIGAALAATLGF
                     AGANLAWQTVFWVGGVVPLILVPLLMRWLPESAVFAGDKQAAPPLRALFAPETATATL
                     LLWLCYFFTLLVVYMLINWLPLLLVEQGFQPSQAAGVMFALQMGAASGTLMLGALMDK
                     LRPVTMSLLIYSGMLASLLALGTVSSFNGMLLAGFVAGLFATGGQSVLYALAPLFYSS
                     QIRATGVGTAVAVGRLGAMSGPLLAGKMLALGTGTVGVMAASAPGILVAGLAVFILMS
                     RRSRMQPCADA"
     gene            complement(8162..9175)
                     /gene="mhpE"
                     /locus_tag="ACHOSC_RS13280"
                     /old_locus_tag="pgaptmp_002656"
     CDS             complement(8162..9175)
                     /gene="mhpE"
                     /locus_tag="ACHOSC_RS13280"
                     /old_locus_tag="pgaptmp_002656"
                     /EC_number="4.1.3.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-2-oxovalerate aldolase"
                     /protein_id="HGU4548571.1"
                     /translation="MNGKKLYISDVTLRDGMHAIRHQYSLENVRQIAKALDDARVDSI
                     EVAHGDGLQGSSFNYGFGAHSDLEWIEAAADVVKHAKIATLLLPGIGTIHDLKNAWQA
                     GARVVRVATHCTEADVSAQHIQYARELGMDTVGFLMMSHMTTPENLAKQAKLMEGYGA
                     TCIYVVDSGGAMNMSDIRDRFRALKAELKPETQTGMHAHHNLSLGVANSIAAVEEGCD
                     RIDASLAGMGAGAGNAPLEVFIAAADKLGWQHGTDLYALMDAADDLVRPLQDRPVRVD
                     RETLALGYAGVYSSFLRHCETAAARYGLSAVDILVELGKRRMVGGQEDMIVDVALDLR
                     NNK"
     gene            complement(9172..10122)
                     /gene="mhpF"
                     /locus_tag="ACHOSC_RS13285"
                     /old_locus_tag="pgaptmp_002657"
     CDS             complement(9172..10122)
                     /gene="mhpF"
                     /locus_tag="ACHOSC_RS13285"
                     /old_locus_tag="pgaptmp_002657"
                     /EC_number="1.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetaldehyde dehydrogenase"
                     /protein_id="HGU4548572.1"
                     /translation="MSKRKVAIIGSGNIGTDLMIKILRHGQHLEMAVMVGIDPQSDGL
                     ASARRMGVATTHEGVIGLMNMPEFADIDIVFDATSAGAHVKNDAALREAKPDIRLIDL
                     TPAAIGPYCVPVVNLEANVDQLNVNMVTCGGQATIPMVAAVSRVARVHYAEIIASIAS
                     KSAGPGTRANIDEFTETTSRAIEVVGGAAKGKAIIVLNPAEPPLMMRDTVYVLSDEAS
                     QDDIEASINEMAEAVQAYVPGYRLKQRVQFEVIPQDKPVNLPGVGQFSGLKTAVWLEV
                     EGAAHYLPAYAGNLDIMTSSALATAEKMAQSLARKAGEAA"
     gene            complement(10119..10928)
                     /gene="mhpD"
                     /locus_tag="ACHOSC_RS13290"
                     /old_locus_tag="pgaptmp_002658"
     CDS             complement(10119..10928)
                     /gene="mhpD"
                     /locus_tag="ACHOSC_RS13290"
                     /old_locus_tag="pgaptmp_002658"
                     /EC_number="4.2.1.80"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308432.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-keto-4-pentenoate hydratase"
                     /protein_id="HGU4548573.1"
                     /translation="MTKHTLEQLAADLRRAAEQGEAIAPLRDLIGIDNAEAAYAIQHI
                     NVQYDVVQGRRVVGRKVGLTHPKVQQQLGVDQPDFGTLFADMCYGDNEIIPFSRVLQP
                     RIEAEIALVLNRDLPATDITFDELYNAIEWVLPALEVVGSRIRDWSIQFVDTVADNAS
                     CGVYVIGGPAQRPAGLDLKNCAMKMTRNNEEVSSGRGSECLGHPLNAAVWLARKMASL
                     GEPLRAGDIILTGALGPMVAVNAGDRFEAHIEGIGSVAATFSSAAPKGSLS"
     gene            complement(10938..11804)
                     /gene="mhpC"
                     /locus_tag="ACHOSC_RS13295"
                     /old_locus_tag="pgaptmp_002659"
     CDS             complement(10938..11804)
                     /gene="mhpC"
                     /locus_tag="ACHOSC_RS13295"
                     /old_locus_tag="pgaptmp_002659"
                     /EC_number="3.7.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414883.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxy-6-oxonona-2,4-dienedioate hydrolase"
                     /protein_id="HGU4548574.1"
                     /translation="MSYQPQTEAATSRFLNVEEAGKTLRIHFNDCGQGDETVVLLHGS
                     GPGATGWANFSRNIDPLVEAGYRVILLDCPGWGKSDSIVNSGSRSDLNARILKSVVDQ
                     LDIAKIHLLGNSMGGHSSVAFTLNWPERVGKLVLMGGGTGGMSLFTPMPTEGIKRLNQ
                     LYRQPTIENLKLMMDIFVFDTSDLTDALFEARLNNMLSRRDHQENFVKSLEANPKQFP
                     DFGPRLAEIKAQTLIVWGRNDRFVPMDAGLRLLSGIAGSELHIFRDCGHWAQWEHADA
                     FNQLVLNFLARP"
     gene            complement(11822..12766)
                     /gene="mhpB"
                     /locus_tag="ACHOSC_RS13300"
                     /old_locus_tag="pgaptmp_002660"
     CDS             complement(11822..12766)
                     /gene="mhpB"
                     /locus_tag="ACHOSC_RS13300"
                     /old_locus_tag="pgaptmp_002660"
                     /EC_number="1.13.11.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-dihydroxyphenylpropionate/2,
                     3-dihydroxicinnamic acid 1,2-dioxygenase"
                     /protein_id="HGU4548575.1"
                     /translation="MHAYLHCLSHSPLVGYVDPAQEVLDEVNGVIASARERIAAFSPE
                     LVVLFAPDHYNGFFYDVMPPFCLGVGATAIGDFGSAAGELPVPVELAEACAHAVMKSG
                     IDLAVSYCMQVDHGFAQPLEFLLGGLDKVPVLPVFINGVATPLPGFQRTRMLGEAIGR
                     FTSTLNKRVLFLGSGGLSHQPPVPELAKADAHMRDRLLGSGKDLPASERELRQQRVIS
                     AAEKFVEDQRTLHPLNPIWDNQFMTLLEQGRIQELDAVSNEELSAIAGKSTHEIKTWV
                     AAFAAISTFGNWRSEGRYYRPIPEWIAGFGSLSARTEN"
     gene            complement(12768..14432)
                     /gene="mhpA"
                     /locus_tag="ACHOSC_RS13305"
                     /old_locus_tag="pgaptmp_002661"
     CDS             complement(12768..14432)
                     /gene="mhpA"
                     /locus_tag="ACHOSC_RS13305"
                     /old_locus_tag="pgaptmp_002661"
                     /EC_number="1.14.13.127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008688 - 3-(3-hydroxyphenyl)propionate
                     hydroxylase activity [Evidence IEA];
                     GO_process: GO:0019622 - 3-(3-hydroxy)phenylpropionate
                     catabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     3-(3-hydroxy-phenyl)propionate/3-hydroxycinnamic acid
                     hydroxylase"
                     /protein_id="HGU4548576.1"
                     /translation="MAIQHPDIQPAVNHSVQVAIAGAGPVGLMMANYLGQMGIDVLVV
                     EKLDKLIDYPRAIGIDDEALRTMQSVGLVENVLPHTTPWHAMRFLTPKGRCFADIQPM
                     TDEFGWPRRNAFIQPQVDAVMLEGVSRFPNVRCLFSRELEAFSQQNDEVTLHLKTAEG
                     QREIVKAQWLVACDGGASFVRRTLNVPFEGKTAPNQWIVVDIANDPLSTPHIYLCCDP
                     VRPYVSAALPHAVRRFEFMVMPGETEEQLREPQNMRKLLSKVLPNPDNVELIRQRVYT
                     HNARLAQRFRIDRVLLAGDAAHIMPVWQGQGYNSGMRDAFNLAWKLALVIQGKARDVL
                     LDTYQQERRDHAKAMIDLSVTAGNVLAPPKRWQGTLRDGVSWLLNYLPPVKRYFLEMR
                     FKPMPQYYGGALVREGEAKHSPVGKMFIQPKVTLENGDVTLLDNAIGANFAVIGWGCN
                     PLWGMSDEQIQQWRALGTRFIQVVPEVQIHTAQDNHDGVLRVGDTQGRLRSWFAQHNA
                     SLVVMRPDRFVAATAIPQTLGNTLNKLASVMTLTRPDADVSVEKVA"
BASE COUNT         3447 a         4011 c         3647 g         3414 t
ORIGIN      
        1 attgcataaa aacattaccc agtcgaacct ccgggtctga aagcccttgc ttttcagtta
       61 ttagcaacat ttaacattta taaattaaca tctggctgcg ccagagatta cgtctctgag
      121 tgcatcgttg tgcttgtagt ataataaata aaatggagtg tcattaagtg gataaggttt
      181 tagattcagc cctcccttct tcggcaaata aaagaaaggg tatccttgct attggcgcac
      241 atcctgacga tatagaatta ggctgtggcg catcgcttgc tcgtcttgcg caaaaaggaa
      301 tttatatcgc agccgtggtg atgactaccg gcaactctgg tacagatgga ataatcgatc
      361 gccatgaaga atcgcgcaac gccctaaaga tattagggtg ccaccaaact attcatctta
      421 attttgctga cacccgcgct catttacagc tcaatgatat gatttccgct ctggaagaca
      481 tcattaaaaa tcaaattcct tctgatgttg aaatcatgcg ggtatatacc atgcatgatg
      541 ccgaccgcca tcaggatcat ctcgctgttt atcaagcttc aatggttgcc tgccgcacta
      601 ttccacaaat tctcggctac gaaaccccaa gtacctggct ttcatttatg cctcaggttt
      661 ttgaatccgt taaagaagaa tatttcacag tcaaacttgc tgcattaaaa aaacataaaa
      721 gccaggaacg acgtgattat atgcgtcatg atcgcctgcg tgcagttgca caatttcgcg
      781 ggcaacaggt caatagcgat ctgggtgaag gctttgtaat tcataaaatg attctttgag
      841 caaattgcat gaaaacctgg atatttatct gtatgtctat agcaatgttg ctatggtttt
      901 taagtacgct aagacgtaaa cccagtcaaa agaaaggctg tattgacgcc attatacctg
      961 cgtataacga aggcccgtgt ctggcgcagt cactggataa tctactgcgt aacccttatt
     1021 tttgccgggt aatttgcgtt aacgacggct ccacggacaa taccgaagcg gtcatggcgg
     1081 aagtcaaacg caaatggggc gaccgctttg ttgccgtcac gcaaaaaaat accggtaaag
     1141 gtggtgcgct gatgaatggc ctcaattatg ccacctgcga ccaggttttt ttaagtgatg
     1201 ccgacaccta tgttccgccc gatcaagacg gaatgggcta tatgctggca gaaattgagc
     1261 gcggtgctga tgccgtaggc ggcattccct ctactgcgtt gaaaggcgcg gggctgttac
     1321 cgcacatccg cgcgaccgta aagttgccga tgattgttat gaagcgcacg ctacagcagc
     1381 tcctgggcgg cgcaccgttt attatcagcg gtgcctgcgg aatgttccgt actgatgtat
     1441 tgcgtaagtt cggtttctcg gatcgtacta aagtcgaaga ccttgatctc acctggacat
     1501 tagtggcaaa cggctaccgt attcggcagg cgaatcgctg catcgtatac ccacaggaat
     1561 gcaacagccc gcgtgaggag tggcgtcgct ggcggcgttg gattgtggga tacgcggtct
     1621 gtatgcgcct gcataaaaga cttttattta gccgcttcgg tatcttcagt atatttccta
     1681 tgctgttggt tgtgctttat ggcgttggga tttatctcac tacctggttt aatgaattca
     1741 tcaccaccgg gccgcatggt gtggtgttgg caatgtttcc gcttatctgg atcggcgtag
     1801 tttgtgttat tggtgctttt agcgcctggt ttcatcgttg ctggttgttg gtgcctttag
     1861 cgccgctttc cgttgtgtat gtattattag cttatgccat ctggattatt tatggactta
     1921 ttgccttttt tactggacgc gaacctcagc gcgacaaacc cacccgctat tccgcactgg
     1981 tggaagcgtc aaccgcttat tcccaacctt ctgtcacagg aactgaaaaa ctatctgaag
     2041 cttaatgtta aagagaaaaa tattcagatt gcagaccagg taattattga tgaaactgca
     2101 ggtgaagttg ttatcggcgc gaatacccgt atttgtcatg gtgccgttat tcagggtccg
     2161 gtagtgattg gcgcaaactg cctgataggt aattatgcgt ttattcgtcc tggcacaata
     2221 atcagcaatg gtgtaaaaat tggttttgcc accgaaatta aaaatgcggt tattgaagcg
     2281 gaagcaacga ttggtccgca atgttttatt gccgactcgg tagttgcaaa ccaggcatat
     2341 ttgggcgcac aagtacgtac cagtaatcat cgtctggatg aacagcctgt gtctgttcga
     2401 actccagagg gaattatcgc taccggatgc gataaattag gttgttatat cgggcagcgt
     2461 tcacgccttg gcgtacaagt tataattttg cctgggcgaa ttatttcccc gaacacgcaa
     2521 cttggcccgc gcgtgattgt agaacgtaat ttacctgccg gaacttattc actccgacaa
     2581 gaacttatcc gtacaggaga ttaaaatgat aaaacggacg ttattagcgg cggccatttt
     2641 tagcgcattg cccgcttatg ccgggttaac ttccattacc gcgggctacg attttaccga
     2701 ttattctggc gatcatggca accgtaattt agcgtatgct gaactggtgg cgaaagttga
     2761 aaacgcaacg ctgcttttta atctttccca ggggcgtcgt gattatgaaa ctgaacattt
     2821 caatgccact cgcggtcagg gtgcggtctg gtataaatgg aataactggc tgacaacccg
     2881 aacgggtatt gcctttgcgg ataatacgcc ggtctttgcc cgccagaatt ttcgtcagga
     2941 tattaacctg gccctgttgc caaaaacgct tttcacgacc ggttatcgct acactaaata
     3001 ttacgatgat gtcgaagtcg atgcctggca aggcggcgta tcactctata ctggcccggt
     3061 gatcaccagc taccgctata cccattatga ctccagcgat gcaggtggta gttatagcaa
     3121 tatgatttcc gtgcgcctga atgacccgcg cggcactggt tatacgcaac tatggctaag
     3181 ccgcggaaca ggcgcttaca cctatgactg gacgccagaa acacgctacg gcagcatgaa
     3241 gagcgtcagt ctgcaacgta ttcaaccgct gactgagcaa cttaatctcg ggctgacggc
     3301 aggtaaagtg tggtacgaca ccccaaccga tgattataac ggtctgcaac ttgcagccca
     3361 tctgacctgg aaattctgat tccttctgcc gcccgctatc cggggcggcc ttccctgccg
     3421 attagccccc ccctttcctc tttgttttcc gaccacattc accggataaa ttttattctc
     3481 cagtgttata tactataggg gggtatgcat tgacatgtag tatacccccc tatagtatat
     3541 tgcgtgcaga tgatgaggtg cgaaatgccc agtactccgg aagagaagaa aaaggtcctt
     3601 actcgagttc gtcgtattcg agggcagatt gatgctctgg aacggtcgat ggagggtgat
     3661 gccgaatgcc gtgccatact ccaacagatc gctgccgttc ggggcgcggc taacgggctg
     3721 atggcagaag tgcttgaaag ccatatccgg gaaacgtttg accgaaatga ctgctacagc
     3781 cgcgaagtca gccaatccgt tgacgacact attgaactgg ttcgagccta tcttaaatag
     3841 ctgaatctat taccatattg aggaagagcg agagatgaaa tcacgtgctg ccgttgcatt
     3901 tgctcccggt aaaccgctgg aaatcgttga aattgacgtt gcaccaccga aaaaaggtga
     3961 agtgctgatt aaagtcaccc ataccggcgt ttgccatacc gacgcattta ccctctccgg
     4021 ggatgacccg gaaggtgtat tcccggtggt tctcggtcac gaaggggccg gcgttgtggt
     4081 tgaagtcggt gaaggcgtaa ccagcgtcaa acctggcgac catgtgatcc cgctttacac
     4141 cgcggagtgc ggcgagtgtg agttctgtcg ttctggcaaa actaacctct gtgttgcggt
     4201 tcgcgaaacc cagggtaaag gcttgatgcc agacggcacc acccgttttt cttacaacgg
     4261 gcagccgctt tatcactaca tggggtgctc tacattcagt gaatacaccg tggtcgcgga
     4321 agtgtctctg gccaaaatta atccagaagc aaaccatgaa cacgtctgtc tgctgggctg
     4381 tggcgtgacc accggtattg gcgcggtgca caacacagct aaagtccagc caggtgattc
     4441 tgttgccgtg tttggtcttg gcgcgattgg tctggcagtg gttcagggcg cgcgtcaggc
     4501 gaaagcggga cggattatcg ctatcgatac caacccgaag aaattcgatc tggcgcgtcg
     4561 cttcggtgct accgactgca ttaacccgaa tgactacgac aaaccgatta aagatgtcct
     4621 gctggatatc aacaaatggg gtatcgacca tacctttgaa tgcatcggta acgtcaacgt
     4681 gatgcgtgcg gcgctggaaa gtgcgcaccg cggctggggt cagtcggtga tcatcggggt
     4741 ggcaggttct ggtcaggaaa tctccacccg tccattccag ttggtcactg gtcgcgtatg
     4801 gaaaggttcc gcgtttggcg gcgtgaaagg tcgttcccag ttaccgggta tggttgaaga
     4861 tgcgatgaaa ggtgatatcg atctggaacc gtttgtcacg cataccatga gccttgatga
     4921 aattaatgac gccttcgacc tgatgcatga aggcaaatcc attcgaaccg taattcgtta
     4981 ctgatttccc gcaggtatac cccgtccact tcagacgggg ttcttaatac tctccctggg
     5041 cagccgtccg ggggattaac cgtgagataa tgactgatgg aactcattga aaaacatgcc
     5101 agctttggcg gctggcaaaa tgtgtatcgg cattattccc aatcactgaa atgtgaaatg
     5161 aatgtcggcg tctatctccc accaaaagcc gcgaatgaaa aattgccggt gctgtactgg
     5221 ctttcgggcc tgacctgcaa cgagcagaat ttcattacta aatcggggat gcagcgttac
     5281 gcggctgaac acaacattat tgttgttgcg ccggacacca gtccgcgagg cagtcatgtc
     5341 gcagatgccg accgttacga tctcgggcaa ggtgccgggt tttacctgaa cgcaacgcaa
     5401 gcgccgtgga atgaacatta caaaatgtat gactatatcc gcaacgagct gccggattta
     5461 gtgatgcaac attttccggc aacgaccaga aagtctatct ctggtcattc tatgggcggg
     5521 ctgggcgcgc tggtgctggc gttacgtaac ccagatgaat atgtcagcgt ctcggcgttt
     5581 tcgcccattg tctccccatc gcaagtaccg tggggacagc aagcctttgc cgcatatctt
     5641 ggtgaaaata aagatgcctg gttgaattac gatccggtga gtcttatttc acaaggtcaa
     5701 cgcgttgcgg aaatcatggt tgatcagggg ttgagtgatg atttttacgc ggaacagctg
     5761 cggactccaa atcttgaaaa gatctgccag gagatgaata tcaagacgtt aatccgttat
     5821 cacgagggtt acgaccacag ctattatttt gtctccagtt ttattggcga gcatattgcc
     5881 taccacgcca ataaactgaa tatgcgttga taatagtgca cgactgccgg atgcggcgtg
     5941 aacgccttat ccgcctacat ttcgcccgca ggccgtaggc atgataagac gcgcatagcg
     6001 tcgcatcagg catctgcgca cgacttatct atgtttacca catcaaatca tcaggcactt
     6061 tgaagtcggc atatgggtcg tcttcatcct gctcttcggc gctgagcgcg ctgtgcaaca
     6121 ctatactgct ggcatcacgc tgagctattt tatcggcaac gctggcggga ataatggcgt
     6181 attcaccttc gctattgtta tcgaccaaca agcgagcaat cgccagacga ccattaatca
     6241 actgcgcctg tgtgagctta tcgacaaaaa tcttcttaat cagattgccg tcagtgaagt
     6301 taaaaccaat atcgccattg gcaatggtga ttcggttcat ttcaatgagc tgcttcacct
     6361 gagctttata ttctttcgcc aacgcagctt gtttttgttg ctcgctaagc tgtttatcac
     6421 gctcaagctg tgccttttta ttttcttcta ccgccgcccg agcttcacgc gcctgaacgc
     6481 gcgatttttt cgccgttctc tccaccttcg ccgctttttt gctggtgact aatcctgctt
     6541 tgagcaactg ctcttgtaag gtaagttttg ccatctttgt ttccaaaccc gctgaataat
     6601 ttttagcgag tatacctgta aacgccgtgg gtgtattggg ggcaactccc tttcgcccct
     6661 gcttaataag gcgcgtcagg catcggcaca cggctgcatt cttgatctcc gactcatcaa
     6721 aataaacacc gccaacccag caacaagaat acccggtgca gaagcggcca ttacgccgac
     6781 cgtgccagtg cctaatgcca gcattttccc ggccagtaac ggaccgctca tagcccccag
     6841 acgccctacc gccacggctg ttcccacacc tgttgcgcgg atctgcgaac tgtaaaacaa
     6901 cggtgccagg gcatacaaaa cgctttgccc acctgtcgca aacaaccccg cgacaaatcc
     6961 cgccagcaac ataccgttaa atgacgacac cgttccaagt gccagcagcg aagctaacat
     7021 gccgctataa atcagtagcg acatggtcac tggacgcagc ttatccatca atgcgcccaa
     7081 cattaacgtc ccgcttgccg cacccatttg cagggcaaac atcacccctg ccgcctgcga
     7141 tggctggaat ccttgctcca ccaaaagcag cggtagccag ttgatcaaca tgtagaccac
     7201 cagcagagtg aagaaataac acaaccacag cagcagcgtc gcggttgccg tttctggcgc
     7261 aaataaggca cgcagtggtg gcgcagcctg tttatcgcca gcgaaaaccg ccgactccgg
     7321 cagccagcgc attaatagcg gcaccagaat caacggcacc acaccaccta cccaaaacac
     7381 cgtttgccat gctaagtttg cccccgcgaa acccagtgtc gccgccagcg ccgcgccaat
     7441 gggaacaccg caatacatca ggctcactgc tgtcccacga aaacgtggac ccgcggcttc
     7501 agacgtcagg gcgataagat tcggcaacgc cgcccccagc ccgacaccgg tcatcagccg
     7561 cgcaaagacc agtgagggga aatcccaggc aatcgccgtt gccagcgaga acaaaccaaa
     7621 cagcgcaact gagccaatca aaatgcgctt gcgaccataa cggtccgcca gcattccgcc
     7681 aaccaacgcg ccgggtagca aaccgagtat tccggcgctg aatatccagc ccatttgcat
     7741 tttatcgagt gcgaaagcct gggcgatgcc acccgccgca atgccagccg cctgaagatc
     7801 cagcccttcc atcagagcga ccaaaaaaca aagcccgata gtcagcatca ggcgggatga
     7861 agatgatgaa ggggtacgag tcgacataag atgaacctcg ttgcgagtgc aggtgaatcg
     7921 cgactgatac attgccagcc gcgtaatcag aactatttaa tgactgccta cgggcgaaat
     7981 gtaggccgga caagacgttt atcgcatccg gcagtcgtgt gcagatgcct gatgcgacgc
     8041 taacgcgtct tatcaggcct acaaattctc acgccacccg taggccggat aaggtgttta
     8101 cgccgcatcc ggcagtcgtg ctgggatggg catacaaaat gcacgttctc ggcagtcatc
     8161 attatttgtt gttgcgcaga tccagcgcca cgtcaacgat catatcctcc tggccgccaa
     8221 ccatccggcg tttgcccagc tcaacgagaa tatccaccgc acttaagcca taacgcgccg
     8281 ccgccgtttc acagtgacgc aggaagctcg agtaaacacc agcgtatccc agcgccagcg
     8341 tttcgcgatc gactcgtacc ggtcgatcct gcaacggacg caccaggtcg tcagcggcat
     8401 ccattaacgc atagagatcg gtcccatgct gccagcccag tttatccgcg gcggcaataa
     8461 atacttccag cggcgcgtta cctgcgcccg cgcccattcc cgcgaggctg gcgtcgattc
     8521 ggtcgcagcc ctcttccacc gccgcgatag agttcgccac gccaagactc aggttatggt
     8581 gagcgtgcat gccagtttgc gtttctggtt tcagctctgc tttcagggcg cggaaacggt
     8641 cacggatatc gctcatgttc atcgcaccgc cagaatccac cacataaata caggtcgcac
     8701 cgtagccttc catcagcttt gcctgctttg cgagattctc cggcgtggtc atatggctca
     8761 tcatcagaaa accaacggtg tccattccga gctcgcgggc atactgaata tgctgggcgg
     8821 aaacatcagc ttcggtacag tgcgttgcca cacgaaccac ccgcgcgcca gcctgccagg
     8881 catttttcag atcgtgaata gtgccgattc ctggcagcaa caacgtcgcg attttggcgt
     8941 gcttcaccac atccgccgct gcttcaatcc attcaaggtc gctatgtgcg ccgaaaccat
     9001 agttaaagct ggaaccctgc aaaccgtcgc catgggccac ttcaatcgaa tccacgcggg
     9061 catcgtccag tgctttggca atctggcgaa cgttttccag cgaatactga tgacgaatgg
     9121 cgtgcatacc gtcacgcaac gtgacgtccg agatataaag ttttttaccg ttcatgccgc
     9181 ttctcctgcc ttgcgcgcca gtgactgggc cattttttcc gctgtcgcca gcgcactgga
     9241 agtcataatg tcgaggttgc ccgcataggc aggcagataa tgcgctgcgc cttcgacttc
     9301 cagccagacc gctgttttca gtccggagaa ttgccccacg cccggtaaat tgaccggttt
     9361 atcctgcggg ataacttcaa actgcacgcg ctgtttcagg cgataacccg gtacgtaagc
     9421 ctgcaccgcc tcagccattt cattgattga ggcttcgata tcatcttgtg aagcttcgtc
     9481 gctcaataca tacaccgtgt cacgcatcat caacggtggc tctgctgggt taagcacaat
     9541 aatcgccttc ccttttgccg cgccgcccac cacttcaatg gctcgggaag tggtttccgt
     9601 aaattcatcg atattggcac gcgtgccagg tccggcagat ttactggcga tagaagcgat
     9661 aatttcggcg taatgaacac gcgccacgcg tgaaactgcc gccaccattg gaatggtggc
     9721 ctggccgccg caggtgacca tgttgacgtt cagttgatcg acgttcgcct cgaggttaac
     9781 caccggcacg cagtaagggc cgatggcagc aggcgtcagg tcaattaagc gaatatccgg
     9841 tttcgcttcg cgtaaagcgg catcgttttt cacatgcgcg cccgcgctgg tcgcatcaaa
     9901 tacaatgtcg atatcagcaa attcaggcat gttcatcagc ccgatcaccc cttcatgggt
     9961 ggtggcgacg cccatacgtc tggcactcgc cagaccgtcg gactgaggat caatgccgac
    10021 catcaccgcc atctccagat gctgaccgtg acgcaaaatt ttaatcatca gatcggtgcc
    10081 aatgttgcca gaaccgataa tggcgacttt acgcttactc atgacagact tccttttggg
    10141 gctgcgcttg aaaatgtcgc agcaactgaa cctatgcctt caatatgggc ttcaaaacga
    10201 tcgcccgcat tcaccgccac catcgggcct aatgccccgg taagaatgat atctccggcg
    10261 cgcagcggtt cacccagact ggccattttg cgtgccagcc agacggcggc attaagcgga
    10321 tgtcccaggc attcgctgcc gcgcccgctg gaaacctctt cgttattacg cgtcatcttc
    10381 atggcgcagt ttttcaggtc taaccccgcc ggacgttgcg ccggaccgcc gatgacatac
    10441 accccgcagg aagcgttatc tgccacggta tcgacaaact gaatcgacca gtcgcgaatg
    10501 cggctcccca ccacttccag cgccggaagt acccattcaa tggcgttata cagttcgtcg
    10561 aaggtgatat cagttgcggg taaatcgcgg ttcaacacca gtgcgatctc cgcttcaatg
    10621 cggggttgca gaacacggga aaaaggaatg atttcgttat cgccataaca catgtcggca
    10681 aataacgtcc caaaatccgg ttgatcaacg cccagttgtt gttgcacttt cggatgcgtc
    10741 aggcccactt tacgccctac cacgcgacgc ccctgcacaa cgtcatattg cacatttatg
    10801 tgctgaatgg cgtaggccgc ttcagcgtta tcgataccaa tcagatcgcg cagcggcgca
    10861 atcgcttcgc cctgctctgc ggcgcggcgt aaatccgccg ccagttgctc aagagtatgc
    10921 ttcgtcatga ccattcctta agggcgtgcg aggaaattca gcaccaattg attgaaagcg
    10981 tcggcatgtt cccactgcgc ccagtgacca cagtcgcgga agatatgcag ttcagaaccg
    11041 gcaatgccgg acagcagacg cagacccgca tccatcggca caaagcggtc gttgcgcccc
    11101 cagacaatca gggtttgcgc tttgatttcc gccagacgtg ggccaaaatc cgggaactgt
    11161 ttcggattag cttccaggct cttaacgaag ttttcctggt gatcgcggcg cgacagcata
    11221 ttattcaggc gcgcttcaaa cagggcgtcg gtcaaatcgc tggtatcgaa aacgaagata
    11281 tccatcatca gcttcaggtt ttcgatagtc ggctgacgat aaagctgatt cagtcgctta
    11341 ataccttcgg ttggcatcgg cgtaaacaaa ctcatgccgc ccgtaccgcc gcccatcagc
    11401 accagtttgc cgacgcgctc cggccagtta agggtgaacg ccacagaact atggccgccc
    11461 atcgagttgc ccagcaggtg gattttggcg atatccagtt gatccaccac gcttttcagg
    11521 attcgtgcat taagatccga tcgtgaacca ctattaacga tcgaatcgct tttgccccaa
    11581 cccggacaat ccagcaggat tacccgatag cccgcctcta ccagcggatc gatattgcgg
    11641 ctgaagttcg cccagccagt agcacccggg ccggaaccat gcagcaggac aacggtttcg
    11701 tcgccttgtc cgcagtcatt aaaatggatg cgcagcgttt tacccgcttc ttctacattc
    11761 agaaaacggc tggtggcggc ttcggtttgt ggctgataac tcatcatctt ctcctgcata
    11821 ttcagttctc tgttctggcg cttaacgagc caaatccggc aatccactcc gggattgggc
    11881 ggtaataacg cccttcgcta cgccagttgc caaatgtaga aatagcggca aaagcggcga
    11941 cccaggtttt gatttcatgt gtcgactttc cggcaatggc ggaaagctct tcgttactga
    12001 cggcatccag ttcctgtatg cgtccctgct ccagcaaagt catgaactgg ttatcccaaa
    12061 tcgggttgag cggatgcaga gttctctgat cctcaacaaa cttctcagcg gcgctaatca
    12121 cccgttgctg acgcaattcg cgctcactgg cgggtaaatc tttcccgctc cccaacagac
    12181 ggtcgcgcat atgggcatcg gctttcgcca gttcgggcac cggcggctga tgggaaagcc
    12241 caccggaacc caggaacagc acgcgtttat tgagagtgct ggtgaaacgt ccaatggctt
    12301 cacccaacat gcgggtacgc tggaaaccgg gcagcggcgt ggcgacaccg ttgatgaaca
    12361 caggcagaac tggcacctta tccagcccac cgagcaggaa ctccagcggc tgggcgaacc
    12421 cgtggtccac ctgcatacag taagaaacgg caagatcgat cccgctcttc atgacagcat
    12481 gcgcacaggc ctccgccagc tccacaggca cgggcagctc tcctgccgca ctgccgaaat
    12541 caccaattgc cgtcgctcca acgcctaaac agaacggtgg catcacgtca tagaaaaagc
    12601 cgttgtagtg atctggcgca aacagcacca ccagttcagg ggagaaagcc gcaatacgct
    12661 cgcgggcgct ggcaatcacg ccattgacct catcgagtac ctcttgcgcc gggtcgacat
    12721 accccaccaa cggcgagtgg gaaagacagt gaagataagc gtgcatatca ggctaccttt
    12781 tcgacagaaa cgtcggcatc agggcgggtc agcgtcatca ccgacgccag tttattcagc
    12841 gtattgccca gggtttgcgg aatggcggtg gcggcaacaa agcgatccgg gcgcatcacc
    12901 accagcgaag cattgtgttg cgcgaaccag ctacgcagac gaccttgcgt atcgcccacg
    12961 cgtagcacgc cgtcgtggtt atcctgtgcg gtatgaattt gcacttccgg caccacctga
    13021 atgaagcgtg tgcccaacgc gcgccactgc tggatttgct cgtcgctcat cccccacagt
    13081 ggattgcatc cccagccaat taccgcgaag ttcgcgccaa tcgcgttatc gagcagcgtc
    13141 acgtcgccgt tttccagcgt gactttcggc tgaataaaca tcttgccgac cggagagtgc
    13201 ttcgcttcgc cctcacgtac cagcgcaccg ccgtaatatt gcggcatcgg cttgaagcgc
    13261 atttcgagga agtagcgttt tactggcggc agataattca gtagccagga aacgccgtca
    13321 cgtaacgtac cctgccagcg tttcggcgga gccagcacgt tgcccgccgt cacggacagg
    13381 tcaatcatcg ctttggcgtg atcgcgacgt tcttgttgat aggtatcgag cagcacatcg
    13441 cgggctttcc cctggataac caacgccagt ttccaggcga ggttaaaggc gtcgcgcata
    13501 ccactgttat agccctgccc ctgccacacc ggcatgatgt gcgcggcatc tcccgccagc
    13561 agtacgcgat caatacggaa acgttgcgcc agtcgcgcgt tatgggtgta gacacgctgg
    13621 cgaatcaatt caacattgtc cggattaggc agcactttgc ttaacagctt gcgcatattt
    13681 tgcggctcac gcagctgctc ttcggtttct cccggcatca ccataaattc aaagcgacgt
    13741 accgcatgag gcagcgcggc agaaacatac gggcgcaccg gatcgcaaca caaatagata
    13801 tgcggcgtac ttaacggatc gttggcgata tctaccacaa tccactgatt tggcgcagtt
    13861 ttaccttcaa acggcacatt cagagtgcga cggacaaaac ttgctccacc gtcacaggct
    13921 accagccact gggctttgac tatttcccgc tgcccttctg ccgttttcag gtgcaaggtc
    13981 acttcgtcat tttgctggct gaaggcctcc agctcgcggg aaaacaagca gcgcacatta
    14041 ggaaaacgcg acaccccttc cagcatcacc gcatcgacct gcggctgaat aaaggcgtta
    14101 cggcgcggcc agccaaattc atcggtcatt ggctgaatat cagcaaaaca gcggcctttc
    14161 ggggtgagaa aacgcatcgc gtgccacggc gtagtgtgcg gcagaacatt ctcgaccagg
    14221 ccaaccgact gcatggtgcg cagcgcctcg tcatcaatac caatcgcacg cgggtagtcg
    14281 atcaacttat cgagtttctc caccaccagc acgtcaatgc ccatctgacc gagatagttc
    14341 gccatcatca gcccgaccgg accggcacct gcgatcgcca cctgaacgct atggttaaca
    14401 gcaggctgga tgtcagggtg ttgtattgcc atttcagtac ctcacgactc ggacaaaatg
    14461 ccgttgcgcg cacagtacgg cgcaacttat tttgttaaaa acatgtaaat gatttttta
//
LOCUS       DAZKHF020000110        14515 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2201S31241
            SAMN43267800-rid25106413.denovo.111, whole genome shotgun sequence.
ACCESSION   DAZKHF020000110 DAZKHF020000000
VERSION     DAZKHF020000110.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267800
            Sequence Read Archive: SRR30307263
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 14515)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14515)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 14515)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350581.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350581.2
            Genome Coverage       :: 151x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:21:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,652
            CDSs (total)                      :: 5,557
            Genes (coding)                    :: 5,320
            CDSs (with protein)               :: 5,320
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 237
            CDSs (without protein)            :: 237
            Pseudo Genes (ambiguous residues) :: 0 of 237
            Pseudo Genes (frameshifted)       :: 102 of 237
            Pseudo Genes (incomplete)         :: 130 of 237
            Pseudo Genes (internal stop)      :: 52 of 237
            Pseudo Genes (multiple problems)  :: 41 of 237
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14515
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267800-rid25106413.denovo.111"
                     /serotype="O 26:H11"
                     /isolate="2201S31241"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-01"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            <1..363
                     /locus_tag="ACHOSC_RS13310"
                     /old_locus_tag="pgaptmp_002662"
     CDS             <1..363
                     /locus_tag="ACHOSC_RS13310"
                     /old_locus_tag="pgaptmp_002662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate decarboxylase"
                     /protein_id="HGU4548577.1"
                     /translation="YQVAAYLADEIAKLGPYEFICTGRPDEGIPAVCFKLKDGEDPGY
                     TLYDLSERLRLRGWQVPAFTLGGEATDIVVMRIMCRRGFEMDFAELLLEDYKASLKYL
                     SDHPKLQGIAQQNSFKHT"
     gene            519..2054
                     /gene="gadC"
                     /locus_tag="ACHOSC_RS13315"
                     /old_locus_tag="pgaptmp_002663"
     CDS             519..2054
                     /gene="gadC"
                     /locus_tag="ACHOSC_RS13315"
                     /old_locus_tag="pgaptmp_002663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid resistance gamma-aminobutyrate antiporter
                     GadC"
                     /protein_id="HGU4548578.1"
                     /translation="MATSVQTGKAKQLTLLGFFAITASMVMAVYEYPTFATSGFSLVF
                     FLLLGGILWFIPVGLCAAEMATVDGWEEGGVFAWVSNTLGPRWGFAAISFGYLQIAIG
                     FIPMLYFVLGALSYILKWPALNEDPITKTIAALIILWALALTQFGGTKYTARIAKVGF
                     FAGILLPAFILIALAAIYLHSGAPVAIEMDSKTFFPDFSKVGTLVVFVAFILSYMGVE
                     ASATHVNEMSNPGRDYPLAMLLLMVAAICLSSVGGLSIAMVIPGNEINLSAGVMQTFT
                     VLMSHVAPEIEWTVRVISALLLLGVLAEIASWIVGPSRGMYVTAQKNLLPAAFAKMNK
                     NGVPVTLVISQLVITSIALIILTNTGGGNNMSFLIALALTVVIYLCAYFMLFIGYIVL
                     VLKHPDLKRTFNIPGGKGVKLVVAIVGLLTSIMAFIVSFLPPDNIQGDSTDMYVELLV
                     VSFLVVLALPFILYAVHDRKGKANTGVTLEPINSQNAPKGHFFLHPRARSPHYIVMND
                     KKH"
     gene            2185..3504
                     /locus_tag="ACHOSC_RS13320"
                     /old_locus_tag="pgaptmp_002664"
     CDS             2185..3504
                     /locus_tag="ACHOSC_RS13320"
                     /old_locus_tag="pgaptmp_002664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="family 10 glycosylhydrolase"
                     /protein_id="HGU4548579.1"
                     /translation="MDICSRNKKLTIRRPAILVALALLLCSCKSTPPESMVTPPAGSK
                     PPATTQQSSQPMRGIWLATVSRLDWPPVSSVNISNPTSRARVQQQAMIDKLDHLQRLG
                     INTVFFQVKPDGTALWPSKILPWSDLMTGKIGENPGYDPLQFMLDEAHKRGMKVHAWF
                     NPYRVSVNTKPGTIRELNSTLPQQPASVYVQHRDWIRTSGDRFVLDPGIPEVQDWITS
                     IVAEVVSRYPVDGVQFDDYFYTESPGSRLNDNETYRKYGGAFASKADWRRNNTQQLIA
                     KVSHTIKSIKPGVEFGVSPAGVWRNRSHDPLGSDTRGAAAYDESYADTRRWVEQGLLD
                     YIAPQIYWPFSRSAARYDVLAKWWADVVKPTRTRLYIGIAFYKVGEPSKIEPDWMING
                     GVQELKKQLDLNDAVPEISGTILFREDYLNKPQTQQAVSYLQSRWGS"
     gene            3881..5263
                     /gene="dosC"
                     /locus_tag="ACHOSC_RS13325"
                     /old_locus_tag="pgaptmp_002665"
     CDS             3881..5263
                     /gene="dosC"
                     /locus_tag="ACHOSC_RS13325"
                     /old_locus_tag="pgaptmp_002665"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase DosC"
                     /protein_id="HGU4548580.1"
                     /translation="MEMYFKRMKDEWTGLVEQADPLIRAKAAEIAVAHAHYLSIEFYR
                     IVRIDPHAEEFLSNEQVERQLKSAMERWIINVLSAQVDDVERLIQIQHTVAEVHARIG
                     IPVEIVEMGFRVLKKILYPVIFSSDYSAAEKLQVYHFSINSIDIAMEVMTRAFTFSDS
                     SASKEDENYRIFSLLENAEEEKERQIASILSWEIDIIYKILLDSDLGSSLPLSQADFG
                     LWFNHKGRHYFSGIAEVGHISRLIQDFDGIFNQTMRNTRNLNNRSLRVKFLLQIRNTV
                     SQIITLLRELFEEVSRHEVGMDVLTKLLNRRFLPTIFKREIAHANRTGTPLSVLIIDV
                     DKFKEINDTWGHNTGDEILRKVSQAFYDNVRSSDYVFRYGGDEFIIVLTEASENETLR
                     TAERIRSRVEKTKLKAANGEDIALSLSIGAAMFNGHPDYERLIQIADEALYIAKRRGR
                     NRVELWKASL"
     gene            5288..7687
                     /gene="dosP"
                     /locus_tag="ACHOSC_RS13330"
                     /old_locus_tag="pgaptmp_002666"
                     /gene_synonym="pdeO"
     CDS             5288..7687
                     /gene="dosP"
                     /locus_tag="ACHOSC_RS13330"
                     /old_locus_tag="pgaptmp_002666"
                     /gene_synonym="pdeO"
                     /EC_number="3.1.4.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416006.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxygen-sensing cyclic-di-GMP phosphodiesterase
                     DosP"
                     /protein_id="HGU4548581.1"
                     /translation="MKLTDADNAADGIFFPALEQNMMGAVLINENDEVMFFNPAAEKL
                     WGYKREEVIGNNIDMLIPRDLRPAHPEYIRHNREGGKARVEGMSRELQLEKKDGSKIW
                     TRFALSKVSAEGKVYYLALVRDASVEMAQKEQTRQLIIAVDHLDRPVIVLDPERHIVQ
                     CNRAFTEMFGYCISEASGMQPDTLLNIPEFPADNRIRLQQLLWKTARDQDEFLLLTRT
                     GEKIWIKASISPVYDVLAHLQNLVMTFSDITEERQIRQLEGNILAAMCSSPPFHEMGE
                     IICRNIESVLNESHVSLFALRNGMPIHWASSSHGAEVQNAQSWSATIRQRDGAPAGIL
                     QIKTSSGAETSAFIERVADISQHMAALALEQEKSRQHIEQLIQFDPMTGLPNRNNLHN
                     YLDDLVDKAVSPVVYLIGVDHIQDVIDSLGYAWADQALLEVVNRFREKLKPDQYLCRI
                     EGTQFVLVSLENDVSNITQIADELRNVVSKPIMIDDKPFPLTLSIGISYDVGKNRDYL
                     LSTAHNAMDYIRKNGGNGWQFFSPAMNEMVKERLVLGAALKEAISNNQLKLVYQPQIF
                     AETGELYGIEALARWHDPQHGHVPPSRFIPLAEEIGEIENIGRWVIAEACRQLAEWRS
                     QNIHIPALSVNLSALHFRSNQLPNQVSDAMQAWGIDGHQLTVEITESMMMEHDTEIFK
                     RIQILRDMGVGLSVDDFGTGFSGLSRLVSLPVTEIKIDKSFVDRCLTEKRILALLEAI
                     TSIGQSLNLTVVAEGVETKEQFEMLRKIHCRVIQGYFFSRPLPAEEIPGWMSSVLPLK
                     I"
     gene            7945..8526
                     /gene="ddpX"
                     /locus_tag="ACHOSC_RS13335"
                     /old_locus_tag="pgaptmp_002667"
     CDS             7945..8526
                     /gene="ddpX"
                     /locus_tag="ACHOSC_RS13335"
                     /old_locus_tag="pgaptmp_002667"
                     /EC_number="3.4.13.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016805 - dipeptidase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine dipeptidase"
                     /protein_id="HGU4548582.1"
                     /translation="MSDTTELVDLAVIFPDLEIELKYACADNITGKAIYQQARCLLHK
                     DAITALAKSISIAQLSGLQLVIYDAYRPQQAQAMLWQACPDPQYVVDVTVGSNHSRGT
                     AIDLTLRDEHGNILDMGAGFDEMHERSHAYHPSVPPAAQRNRLLLNAIMTGGGFVGIS
                     SEWWHFELPQAASYPLLADQFSCFISPGTQHVS"
     gene            8540..10090
                     /locus_tag="ACHOSC_RS13340"
                     /old_locus_tag="pgaptmp_002668"
     CDS             8540..10090
                     /locus_tag="ACHOSC_RS13340"
                     /old_locus_tag="pgaptmp_002668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HGU4548583.1"
                     /translation="MKRSISFRPTLLALVLATNFPVSHAAVPKDMLVIGKAADPQTLD
                     PAVTIDNNDWTVTYPSYQRLVQYKTDGDKGSTDVEGDLASSWKASDDQKEWTFTLKDN
                     AKFADGTPVTAEAVKLSFERLLKIGQGPAEAFPKDLKIDAPDEHTVKFTLSQPFAPFL
                     YTLANDGASIINPAVLKEHAADDARGFLAQNTAGSGPFMLKSWQKGQQLVLVPNPHYP
                     GNKPNFKRVSVKIIGESASRRLQLSRGDIDIADALPVDQLNALKQENKVNVAEYPSLR
                     VTYLYLNNSKAPLNQADLRRAISWSTDYQGMVNGILSGNGKQMRGPIPEGMWGYDATA
                     MQYNHDETKAKAEWDKVTSKPTSLTFLYSDNDPNWEPIALATQSSLNKLGINVKLEKL
                     ANATMRDRVGKGDYDIAIGNWSPDFADPYMFMNYWFESDKKGLPGNRSFYENSEVDKL
                     LRNALATTDQTQRTRDYQQAQKIVIDDAAYVYLFQKNYQLAMNKEVKGFVFNPMLEQV
                     FNINTMSK"
     gene            10092..11114
                     /locus_tag="ACHOSC_RS13345"
                     /old_locus_tag="pgaptmp_002669"
     CDS             10092..11114
                     /locus_tag="ACHOSC_RS13345"
                     /old_locus_tag="pgaptmp_002669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HGU4548584.1"
                     /translation="MTFWSILRQRCWGLVLVVAGVCVITFIISHLIPGDPARLLAGDR
                     ASDAIVENIRQQLGLDQPLYVQFYRYVSDLFHGDLGTSIRTGRPVLEELRIFFPATLE
                     LAFGALLLALLIGIPLGILSAVWRNRWLDHLVRIMAITGISTPAFWLGLGVIVLFYGH
                     LQILPGGGRLDDWLDPPTHVTGFYLLDALLEGNGEVFFNALQHLILPALTLAFVHLGI
                     VARQIRSAMLEQLSEDYIRTARASGLPGWYIVLCYALPNALIPSITVLGLALGDLLYG
                     AVLTETVFAWPGMGAWVVTSIQALDFPAVMGFAVVVSFAYVLVNLVVDLLYLWIDPRI
                     GRGGGE"
     gene            11111..12007
                     /gene="ddpC"
                     /locus_tag="ACHOSC_RS13350"
                     /old_locus_tag="pgaptmp_002670"
     CDS             11111..12007
                     /gene="ddpC"
                     /locus_tag="ACHOSC_RS13350"
                     /old_locus_tag="pgaptmp_002670"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D,D-dipeptide ABC transporter permease"
                     /protein_id="HGU4548585.1"
                     /translation="MMLSEETSAVRPQKQTRFNGAKLVWMLKGSPLTVTGAVIIVLML
                     LMMIFSPWLATHDPNAIDLTARLLPPSAAHWFGTDEVGRDLFSRVLVGSQQSILAGLV
                     VVAIAGMIGSLLGCLSGVLGGRADAIIMRIMDIMLSIPSLVLTMALAAALGPSLFNAM
                     LALAIVRIPFYVRLARGQALVVRQYTYVQAAKTFGASRWHLISWHILRNSLPPLIVQA
                     SLDIGSAILMAATLGFIGLGAQQPSAEWGAMVANGRNYVLDQWWYCAFPGAAILLTAV
                     GFNLFGDGIRDLLDPKAGGKQS"
     gene            12004..12990
                     /locus_tag="ACHOSC_RS13355"
                     /old_locus_tag="pgaptmp_002671"
     CDS             12004..12990
                     /locus_tag="ACHOSC_RS13355"
                     /old_locus_tag="pgaptmp_002671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HGU4548586.1"
                     /translation="MTQPVLDIQQLHLSFPGFNGDVHALNNVSLQINRGEIVGLVGES
                     GSGKSVTAMLIMRLLQTGSYCVHRGQISLLGDDVLNAREKQLRQWRGARVAMIFQEPM
                     TALNPTLRIGLQMMDVIRHHQPISRREARAKAIALLEEMQIPDAVEVMSRYPFELSGG
                     MRQRVMIALAFSCEPQLIIADEPTTALDVTVQLQVLRLLKHKARASGTAVLFISHDMA
                     VVSQLCDSVYVMYAGSVIESGVTADVIHHPRHPYTIGLLQCAPEHGVPRQLLPAIPGT
                     VPNLTHLPEGCAFRDRCYAAGAQCENVPALTACGDNNQRCACWYPQQEVISV"
     gene            12983..13909
                     /locus_tag="ACHOSC_RS13360"
                     /old_locus_tag="pgaptmp_002672"
     CDS             12983..13909
                     /locus_tag="ACHOSC_RS13360"
                     /old_locus_tag="pgaptmp_002672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015440 - ABC-type peptide transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015833 - peptide transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptide/dipeptide ABC transporter
                     ATP-binding protein"
                     /protein_id="HGU4548587.1"
                     /translation="MSDTLLTLRDVHINFPARKNWLGKTTEHVHAINGIDLQIRRGET
                     LGIVGESGCGKSTLAQLLMGMLQPSHGQYIRSGSQRIMQMVFQDPLSSLNPRLPVWRI
                     ITEPLWIAKRSSEQQRRALAEELAVQVGIRPEYLDRLPHAFSGGQRQRIAIARALSSQ
                     PDVIVLDEPTSALDISVQAQILNLLVTLQENHGLTYVLISHNVSVIRHMSDRVAVMYL
                     GQIVELGEAQQVLTVPAHPYTRLLLDSLPAIDKPLEEEWALRKTDLPGNRTLPQGCFF
                     YERCPLATHGCEVRQSLTIREDGREIRCWRAL"
     gene            complement(13965..14396)
                     /gene="osmC"
                     /locus_tag="ACHOSC_RS13365"
                     /old_locus_tag="pgaptmp_002673"
     CDS             complement(13965..14396)
                     /gene="osmC"
                     /locus_tag="ACHOSC_RS13365"
                     /old_locus_tag="pgaptmp_002673"
                     /EC_number="1.11.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxiredoxin OsmC"
                     /protein_id="HGU4548588.1"
                     /translation="MTIHKKGQAHWEGDIKRGKGTVSTESGVLNQQPYGFNTRFEGEK
                     GTNPEELIGAAHAACFSMALSLMLGEAGFTPTSIDTTADVSLDKVDAGFAITKIALKS
                     EVAVPGIDASTFDGIIQKAKAGCPVSQVLKAEITLDYQLKS"
BASE COUNT         3552 a         3605 c         3692 g         3666 t
ORIGIN      
        1 taccaggttg ccgcttatct ggcggatgaa atcgccaaac tggggccgta tgagttcatc
       61 tgtacgggtc gcccggacga aggcatcccg gcggtttgct tcaaactgaa agatggtgaa
      121 gatccgggat acaccctgta cgacctctct gaacgtctgc gtctgcgcgg ctggcaggtt
      181 ccggccttca ctctcggcgg tgaagccacc gacatcgtgg tgatgcgcat tatgtgtcgt
      241 cgcggcttcg aaatggactt tgctgaactg ttgctggaag actacaaagc ctccctgaaa
      301 tatctcagcg atcacccgaa actgcagggt attgcccagc agaacagctt taaacatacc
      361 tgataacgtt taacggtaac ggtgtcccga aacgaacccg tttcgggaca atttccaaag
      421 tctgttcact ggcattagca acggaaaata ttgttctgaa tacgcttcag aacaaaacag
      481 gtgcggttcc gacaggaata ccgttttagg gggataatat ggctacatca gtacagacag
      541 gtaaagctaa gcagctcaca ttactcggat tctttgccat aacggcatcg atggtaatgg
      601 ctgtttatga ataccctacc ttcgcaacat cgggcttttc attagtcttc ttcctgctat
      661 taggcgggat tttatggttt attcccgtgg gactttgtgc tgcggaaatg gccaccgtcg
      721 acggctggga agaaggtggt gtcttcgcct gggtatcaaa tactctgggg ccgagatggg
      781 gatttgcagc gatctcattt ggctatctgc aaatcgccat tggttttatt ccgatgctct
      841 atttcgtgtt aggggcactc tcctacatcc tgaaatggcc agcgctaaat gaagacccca
      901 ttaccaaaac tattgcagca ctcatcattc tttgggcgct ggcattaacg cagtttggtg
      961 gcacgaaata cacggcgcga attgctaaag ttggcttctt cgccggtatc ctgttacctg
     1021 catttatttt gatcgcatta gcggctattt atctgcactc cggtgccccc gttgctatcg
     1081 aaatggattc gaagaccttc ttccctgact tctctaaagt gggcaccctg gttgtatttg
     1141 ttgccttcat tttgagttat atgggcgtag aagcttccgc aacccacgtc aatgaaatga
     1201 gtaacccagg gcgcgactat ccactggcta tgttactgct gatggtggcg gcaatctgct
     1261 taagctctgt tggtggtttg tctattgcga tggtcattcc gggtaatgaa atcaacctct
     1321 ccgcaggggt aatgcaaacc tttaccgttc tgatgtccca tgtggcaccg gaaattgagt
     1381 ggacggttcg cgtgatctcc gcactgctgt tgctgggtgt tctggcggaa atcgcctcct
     1441 ggattgttgg tccttctcgc gggatgtatg tcacagcgca gaaaaacctg ctgccagcgg
     1501 cattcgctaa aatgaacaaa aatggcgtac cggtaacgct ggtcatttcg cagctggtga
     1561 ttacttctat cgcgttgatc atcctcacca ataccggtgg cggtaacaac atgtccttcc
     1621 tgatcgctct ggcgctgacg gtggtgattt atctgtgtgc ttatttcatg ctgtttattg
     1681 gctacattgt gttggttctt aaacatcctg acttaaaacg cacatttaat atccctggtg
     1741 gtaaaggggt gaaactggtc gtggcaattg tcggtctgct gacttcaatt atggcgttta
     1801 ttgtttcctt cctgccgccg gataacatcc agggtgattc taccgatatg tatgttgaat
     1861 tactggttgt tagtttcctg gtggtacttg ccctgccctt tattctctat gctgttcatg
     1921 atcgtaaagg caaagcgaat accggcgtca ctctggagcc aatcaacagt cagaacgcac
     1981 caaaaggtca cttcttcctg cacccgcgtg cacgttcacc acactatatt gtgatgaatg
     2041 acaagaaaca ctaaccatgt ctgaatggtt ataagacaag ggagcgataa gtcatcgctc
     2101 cctttttcgt gcttgctgtc gtattgactt aaccgggata agtacgagaa tgagcgcaca
     2161 tctgtttacc ggaaaccagc acatatggat atctgctccc gaaacaagaa attaacgatt
     2221 agaagaccag cgatactagt tgcactggca cttttactgt gtagttgtaa aagcacgcct
     2281 ccagagtcca tggtgacacc accagcaggt tcaaagccac cagccacgac gcaacaatcg
     2341 tcacaaccga tgcgtggcat ctggctggcc acggtttctc ggctcgactg gccaccggtt
     2401 tcctcggtta acattagtaa ccccaccagc cgggcccgtg tacaacaaca ggcgatgatc
     2461 gacaaactgg atcatctgca acgtctcggc ataaacacgg tctttttcca ggtcaagccg
     2521 gacggtaccg ccctgtggcc atcgaaaatt ttgccgtggt ccgatcttat gaccggtaag
     2581 attggtgaaa atccgggtta cgatccgctg caatttatgc tcgacgaagc ccacaagcgt
     2641 gggatgaaag tacacgcctg gtttaacccc tatcgcgtat cggttaatac gaagcccggt
     2701 actatcaggg aactgaatag cactctgcct caacaaccgg cgagcgtcta tgtgcaacac
     2761 cgcgactgga tcagaacgtc tggcgatcgc tttgtcctcg acccgggcat ccctgaggtt
     2821 caggactgga tcacatcaat agtcgcagaa gtggtttccc gctatcccgt agatggcgtg
     2881 cagtttgacg actatttcta tacggagtca ccgggttcac ggctaaatga taacgaaacg
     2941 taccgtaaat acggaggcgc atttgcgtca aaagcagact ggcggcgcaa caatactcag
     3001 cagttaattg caaaggtatc gcacaccatt aaaagcatta agccgggagt cgaatttggt
     3061 gttagcccgg caggcgtgtg gcgtaaccga tcacacgatc cgctcggttc cgatacccga
     3121 ggcgcggcag cctatgacga atcctacgct gacacccgtc gatgggtgga acaaggattg
     3181 ctggattaca ttgctcccca aatttactgg ccgttctcac ggagtgccgc gcgttatgac
     3241 gtgttggcaa aatggtgggc ggatgtcgtt aaaccgacca ggacccgcct gtatatcggt
     3301 atcgccttct ataaagtggg tgaaccttca aagatagagc cagactggat gattaacggc
     3361 ggcgtacagg aactgaaaaa gcagctcgat cttaacgatg cggtaccaga aattagcggc
     3421 accatcttgt tccgtgagga ctatctgaat aaaccacaga ctcaacaagc ggtcagctat
     3481 ctgcaaagtc gttggggcag ttaaactccc attcttactt tgtaacaagc cggtgcttta
     3541 cccaccggct tgttaccctc ttgaactatc ccactactaa tcatgcttac taaagtcaaa
     3601 ttaaccacgc aattaaccac acttagttaa acagccataa atcccctaat aaaaatgtat
     3661 gaatatgaaa aaacatacga tattcgctgt caccgaaaaa taattacgat gggctatact
     3721 cttctgaaga acagtagcgt agattaaaac tgtcctaagt cgcttatttc ttttcagtat
     3781 atcttcatat ttcaggagaa tatggtgcag ttagtcgatc tcgcacgttg cgtttcgttt
     3841 tgattccgtc tgatttccca gccttataag ggtgtgaatt atggagatgt attttaaaag
     3901 aatgaaagat gagtggaccg gacttgtcga acaggcagat cctctcattc gtgctaaagc
     3961 cgcagaaatt gccgttgcgc atgctcatta tctgagtatc gagttttatc gaattgtccg
     4021 catcgacccg catgccgaag aattcttgag taatgaacaa gttgagcggc agttgaaaag
     4081 tgcgatggaa cgctggatta ttaacgtgct ttctgcccag gttgacgatg tcgaaaggct
     4141 aatacaaatc cagcataccg tcgcggaagt gcatgcccgc ataggaattc cggtagaaat
     4201 tgtcgagatg gggtttcggg tgctgaaaaa aatcctctat ccggtaatct tctcttcgga
     4261 ttattctgcc gcagaaaaac ttcaggtcta ccatttctcg attaacagta ttgatatcgc
     4321 tatggaagtg atgacccgcg cgtttacctt tagtgacagt agtgcctcaa aggaagatga
     4381 aaactatcgt atcttctcgt tactagaaaa cgccgaagaa gaaaaagaac ggcaaatagc
     4441 ctcaatactt tcatgggaaa tagatattat ctataaaatc ctgctggatt ctgatttagg
     4501 cagtagtttg cctttaagcc aggctgattt tggcctgtgg tttaaccata aaggtcgaca
     4561 ttattttagt ggcattgccg aagtaggcca tatctcccgt ctgattcagg atttcgacgg
     4621 tatattcaat caaaccatgc gtaacaccag gaatttgaat aacagaagtc tgcgggtgaa
     4681 atttttatta cagataagaa atactgtatc gcaaattatt accttgctgc gtgaattgtt
     4741 tgaagaagta tcgcgccacg aagtcggtat ggatgtactg acgaaattac ttaaccgccg
     4801 tttcctgccg actatcttca aacgcgaaat tgcccatgcc aaccggaccg gtacaccact
     4861 gtcagtgctg attattgacg ttgataaatt caaagagatc aacgatacgt ggggccataa
     4921 cactggcgat gaaattctgc gtaaagtctc tcaggccttt tatgacaacg tccgcagtag
     4981 tgattatgtt tttcgctacg ggggcgatga atttatcatt gttttgactg aagcttctga
     5041 aaacgaaacg ttacgtaccg cagaacgtat tcgcagtcgg gtggagaaaa ccaaactgaa
     5101 agccgcaaac ggcgaggata ttgccctctc actttccatc ggtgccgcca tgtttaatgg
     5161 tcatcctgac tatgagcgcc tcattcaaat agccgatgaa gctctgtata tcgccaaaag
     5221 acgaggtaga aaccgtgttg aactctggaa agccagtctt tagatgcgcc aggatgcaga
     5281 ggtaatcatg aagctaaccg atgcggataa tgccgccgat ggcatttttt tccccgccct
     5341 tgagcaaaat atgatgggtg cggtgttaat taacgaaaat gatgaagtga tgtttttcaa
     5401 ccccgccgca gagaagctct gggggtacaa acgtgaagaa gtcattggca ataacattga
     5461 tatgctgatt ccgcgggatt tgcgtcctgc gcatcctgaa tacattcgtc ataaccgtga
     5521 aggcggtaaa gcgcgtgtcg aggggatgag tcgggagctg cagctggaga aaaaagacgg
     5581 cagtaaaatc tggacccgtt ttgcgctatc gaaagtgagc gccgagggga aagtttatta
     5641 cctggcgctg gtacgggatg ccagcgtaga aatggcgcaa aaagaacaga ctcgacaatt
     5701 gattattgcc gttgaccatc tcgaccgacc ggtgattgtc ctcgatccgg aacgccatat
     5761 tgtgcagtgc aatcgcgcat ttaccgaaat gtttggttac tgcattagcg aagccagcgg
     5821 tatgcagccc gatacactcc tgaacattcc tgaattccct gccgataacc gcattcgttt
     5881 acaacagttg ctatggaaaa ccgcccgcga tcaggacgaa tttctgctgt tgacgcgcac
     5941 cggtgaaaaa atctggatta aagcctctat cagcccggtt tatgacgtgc tcgcgcatct
     6001 gcagaacctg gtaatgactt tctcggatat caccgaagaa cggcaaatcc gccagcttga
     6061 aggcaatatt ctcgccgcca tgtgcagcag cccgccattt catgaaatgg gggaaatcat
     6121 ttgtcgtaac atcgaatctg ttctcaacga atcgcatgtt tcgctgttcg cactgcgcaa
     6181 cgggatgccg atacactggg cgtcatcttc gcacggtgca gaagttcaaa atgcgcaaag
     6241 ctggtcagcg accattcgtc agcgtgatgg cgcgcctgcg gggatcctgc aaattaaaac
     6301 ctcgtcagga gcagaaacca gcgcctttat cgaacgcgtg gcagatatca gtcagcatat
     6361 ggccgcgctg gcgctggaac aggaaaaaag ccgccagcat attgaacaac tcatccaatt
     6421 tgatccgatg accggtctgc caaatcgcaa taacctgcac aattacctcg atgacctggt
     6481 cgacaaagcc gtctctccgg tggtgtatct catcggtgtt gaccatattc aggatgtgat
     6541 tgatagcctt ggctatgcgt gggccgatca ggcattgctg gaagtggtca atcgctttcg
     6601 tgaaaaactc aaaccggatc agtatctctg tcgtatcgaa ggtacgcagt ttgtcctcgt
     6661 gagcctggaa aacgacgtca gtaacattac ccagatcgcc gatgagctac ggaatgtggt
     6721 cagcaagccg ataatgattg acgataaacc attcccgctt accttgagta ttggcatcag
     6781 ctacgacgtg ggtaaaaacc gcgattacct actctccact gctcacaatg caatggatta
     6841 tattcgcaag aatggcggta acggctggca gttcttcagc ccggcgatga acgaaatggt
     6901 aaaagagcgt ttggttttag gcgcagcgct gaaagaagcg attagcaata accaactgaa
     6961 actggtttac cagccgcaaa tcttcgcaga aacgggtgaa ctgtacggca tcgaagccct
     7021 tgctcgctgg cacgatcccc agcatggtca tgtaccccct tcacggttta ttcctctcgc
     7081 agaagagatt ggtgaaatcg aaaatattgg gcgctgggtc atcgcggaag cttgccgtca
     7141 gttagcagaa tggcgtagcc agaatattca tatcccggcg ttatccgtga acttgtcggc
     7201 gctgcacttt cgcagtaatc agctgcctaa tcaggtgtct gatgcaatgc aagcctgggg
     7261 tattgacggc caccagctga cggtagaaat cacggaaagc atgatgatgg aacacgatac
     7321 cgaaatcttt aagcgcattc agatcctgcg tgatatgggc gtgggcttat cggtagatga
     7381 ttttggtacg ggcttttccg gattatcccg cttagtcagt cttccggtaa cggaaatcaa
     7441 aattgacaaa agttttgtcg atcgttgtct gaccgaaaaa cgcatccttg ccttacttga
     7501 agccattacc agcattgggc aaagcctcaa tttaaccgtc gtggcggaag gcgtcgaaac
     7561 caaagagcaa tttgagatgc tacgcaagat ccactgtcgc gttattcagg gatatttctt
     7621 ttcccgcccc ctacccgccg aagaaattcc aggctggatg agcagcgtgt taccgctgaa
     7681 aatctgacaa attcctctcg cccgcactcg cgggttttca tttaacgtga cactgtcact
     7741 taaatactgt gatttcagct acgattccgg gagattcctt ccttaacttc ccctcttgca
     7801 ccaaaactgc actacgctgc acaaatgttg atcaaaagtt aaaaaatata actttaagcc
     7861 agcattctgg cgcttatccc ggcatggcat gagatctgca taagcggaaa gcgcagcaat
     7921 ttttgtctta tacaggaacc gtttatgtcg gataccaccg aactggttga tttagccgta
     7981 atcttccctg atctggagat cgaattgaaa tacgcctgcg ctgataacat cacaggtaaa
     8041 gctatttatc agcaagcgcg ttgtctgcta cacaaggatg cgattaccgc gctggcgaaa
     8101 agtatcagta tcgcccagct gtcaggctta caactggtga tttacgatgc gtatcgccca
     8161 caacaagcac aggcgatgtt gtggcaagcc tgcccagacc cgcaatatgt tgttgatgtg
     8221 acggtcggtt ctaatcacag ccgtggcacg gcgatcgacc tgacgcttcg tgatgagcac
     8281 gggaacatcc tcgatatggg cgcagggttc gatgaaatgc acgagcgctc ccatgcctat
     8341 cacccttccg tcccgcccgc cgctcagcgc aatcggctgt tgctgaatgc gataatgact
     8401 ggtggcggtt ttgtcggtat ctccagcgaa tggtggcact tcgaattacc ccaggcagcg
     8461 agttaccctc tgctcgctga tcaattctct tgttttatat cccctggcac acagcacgtc
     8521 agttaatcag gaaactgtca tgaagagatc gatatcgttt cgtcccacat tgctcgcgct
     8581 cgtccttgcc acaaatttcc cggtttcgca cgccgccgta ccgaaagata tgctggtgat
     8641 tggtaaggcc gccgatccac aaaccctcga cccggcggta acaatagata ataacgactg
     8701 gacggtgacc tacccgtctt atcagcggct ggttcagtac aaaacggacg gtgataaagg
     8761 ctcaaccgac gttgaaggcg atctggcaag tagctggaaa gcgtctgacg atcaaaaaga
     8821 gtggacgttc accctgaaag ataatgctaa atttgccgat ggcacacctg tcactgccga
     8881 agcagtaaaa ctttcttttg agcggctact aaaaatcggc caggggccag cagaagcatt
     8941 tcccaaagat ttaaagattg atgctcccga cgaacatacg gtgaagttta cccttagcca
     9001 accattcgca ccgttcctct acacgctggc gaatgacggt gcatccatta tcaatccggc
     9061 ggtcttaaag gaacatgcag cggatgatgc tcgcggcttc ctcgcgcaaa ataccgccgg
     9121 ttccggacca tttatgctga aaagctggca aaaaggtcag caattagttc tggtgccaaa
     9181 tccgcattac cccggcaata aaccgaactt taagcgagta tcggtaaaaa ttatcggtga
     9241 aagtgcctcc cgtcgcctgc agctctcccg tggcgacatt gacattgccg atgcgctgcc
     9301 ggtggatcaa ctcaacgccc tgaagcagga aaataaagtc aatgtggcag agtatccgtc
     9361 actgcgcgtt acctatctgt atctgaataa cagcaaagcg cctcttaatc aggcggatct
     9421 acgtcgggcc atttcctggt ctaccgatta tcagggcatg gttaacggca ttctgagtgg
     9481 taacggaaaa cagatgcgcg gcccgattcc ggaaggcatg tggggctacg atgcgacggc
     9541 aatgcaatac aaccatgacg aaacgaaagc caaagccgaa tgggataaag tgacgagcaa
     9601 acccaccagc ctgacgtttc tctactccga taacgatccg aactgggagc ctattgcttt
     9661 ggcgacacaa tccagtctca acaagctggg tatcaatgtg aagctggaaa agctggcgaa
     9721 cgccaccatg cgcgacagag tgggtaaagg tgattacgac attgcgattg gcaactggag
     9781 tccggatttt gccgacccgt atatgtttat gaattactgg tttgagtcag acaaaaaagg
     9841 tctgccgggt aaccgctcgt tctatgaaaa cagtgaggtc gataagttac tgcgcaatgc
     9901 gcttgcgacc accgaccaga cgcagcgtac ccgggactac cagcaggcac agaaaatcgt
     9961 cattgatgac gctgcttatg tgtacctgtt ccagaaaaac taccaactgg cgatgaacaa
    10021 agaggtgaaa ggctttgtgt tcaatcccat gctggaacag gtcttcaata tcaataccat
    10081 gagtaaataa cgtgaccttc tggagtattt tacgccaacg ctgctggggg ctggtgctcg
    10141 tggtggcggg cgtctgcgtg attacgttta ttatctcgca tctgatccct ggcgatccgg
    10201 cgcggttact ggcgggtgac cgcgccagcg atgctatcgt ggaaaatatt cgccagcaac
    10261 tgggactgga ccagccactg tatgtacagt tttaccgcta cgtcagcgac ctgtttcatg
    10321 gtgacctggg aacatccatt cgtaccgggc gtccggtgct ggaagagttg cgtatatttt
    10381 tcccggcgac gctggagctg gcttttggtg ccctgctgct ggcactcctg attggcatcc
    10441 cgctgggcat actctctgca gtctggcgaa atcgctggct ggatcatctg gtgcgaataa
    10501 tggccattac cggaatctcc acacctgcgt tctggcttgg gctgggcgtc attgtgctgt
    10561 tttatggtca tctgcaaatt cttcccggcg gcggaaggct tgatgactgg ctggatccac
    10621 caacgcacgt taccggcttt tatctgctcg atgcgctgct tgaaggcaac ggtgaagtct
    10681 tcttcaatgc gttgcaacat ctcatcttac cggcattaac gctggcgttc gttcacctgg
    10741 gaattgtcgc tcgccagatc cgctcagcga tgctggaaca attgagtgaa gactacattc
    10801 gtaccgcccg ggccagcggc ttgcccggct ggtatatcgt tttatgttat gcgctaccca
    10861 atgcgttgat cccatcgatt accgtattgg gtttggcgct gggcgatttg ttgtatggcg
    10921 cagtgctcac cgaaaccgtt tttgcctggc ccggaatggg tgcatgggta gtaacatcaa
    10981 tacaggcgct cgacttcccg gcagtgatgg gctttgccgt cgtggtttca tttgcttatg
    11041 tgctggtcaa cctggtggtg gatttgctct atttgtggat tgatccgcgt atcggacgtg
    11101 gaggtggtga atgatgctaa gcgaggaaac gtccgccgta cgcccacaaa aacaaacgcg
    11161 atttaacggt gcaaaactgg tatggatgct gaaaggcagt ccgctcaccg tgaccggcgc
    11221 agtcatcatt gtattaatgc tattgatgat gattttttca ccgtggctgg cgacgcatga
    11281 tcccaacgcc attgatttaa ccgcccgcct tttgccgcct tctgcggcgc actggtttgg
    11341 caccgatgaa gtgggacgcg atctgttcag ccgcgtactg gtcggcagtc agcaatcaat
    11401 tctcgccgga ttagtcgtgg ttgccattgc gggtatgatt ggttcgctac tcggatgtct
    11461 atccggtgtg cttggcggac gcgcagacgc catcatcatg cgcatcatgg acattatgct
    11521 gtcgattcct tcgttggtac tgacaatggc actggctgcc gctctcgggc cgagtttatt
    11581 taacgccatg ctggcgcttg ctattgtgcg aattcccttt tatgtgcgcc tggcgcgggg
    11641 gcaagcatta gttgtacgcc agtataccta tgttcaggcg gcgaaaacct ttggtgcgtc
    11701 gcgttggcat ctgatcagct ggcatatttt acgtaactcc ctgccgccgc tgatcgtgca
    11761 ggcatcgctg gatatcggta gcgcgatttt aatggccgcc acgttgggat ttattggcct
    11821 gggtgctcaa caaccgagtg ctgaatgggg cgcgatggta gcgaatggtc gcaactatgt
    11881 actcgatcag tggtggtatt gcgcatttcc gggggcagcg attttgctta ccgccgttgg
    11941 gtttaatctc tttggtgatg gtattcgcga tctgcttgac ccgaaagcag gaggaaagca
    12001 gtcatgaccc aacccgttct ggacattcaa cagctgcatt tgagtttccc cggttttaac
    12061 ggtgatgttc acgcgctcaa caatgtgtcc ttgcagatta accgcggcga aattgtcggt
    12121 ctggtgggag aatccggctc aggtaaatca gtcaccgcaa tgctgattat gcgtctgcta
    12181 cagacgggca gttattgcgt acatcggggg cagatttcac tgctgggaga cgatgttctt
    12241 aacgctcggg aaaaacagct tcgtcagtgg cgcggcgcac gagtggcgat gatctttcag
    12301 gaaccgatga ccgccctcaa tccgacactt cgaataggtc ttcagatgat ggacgtgatc
    12361 cgccatcatc aaccaataag tcgtcgggaa gccagagcta aagcgattgc cctgctggaa
    12421 gagatgcaaa tcccggatgc cgtggaagtt atgtcgcgct atccgtttga gctttcaggt
    12481 ggtatgcgcc agcgggtaat gattgcgctg gcattctcct gcgagccgca attgattatt
    12541 gccgacgaac cgactacggc gctggacgtc acggtgcagt tgcaggtact gcgtttgctt
    12601 aaacataaag cccgcgccag tggaactgcg gttctgttca tcagccatga tatggccgtg
    12661 gtgtcgcaac tgtgcgatag cgtttacgtg atgtatgccg gaagcgtaat tgaaagcggc
    12721 gtgacggcag atgttatcca tcatccccgg catccgtata ccattggttt gctccaatgc
    12781 gcaccagaac atggagtacc acgccagcta ttacccgcca ttccagggac ggtaccaaac
    12841 ctcacccatt tgcctgaagg ctgcgctttt cgcgatcgtt gctatgcggc aggtgcacag
    12901 tgtgaaaacg tcccggcgct gacagcgtgt ggtgacaaca accagcgctg cgcctgttgg
    12961 tatccgcagc aggaggtcat aagtgtctga cacgttatta acgttacgcg acgtccatat
    13021 caatttcccg gcccgtaaaa actggcttgg taaaactacg gaacatgttc atgccattaa
    13081 tggtattgat ttacagatcc gccgtggtga aaccttaggg atcgtcggcg agtcaggctg
    13141 cggcaaaagc accctcgcac agcttttaat gggcatgctg caaccgagcc acgggcagta
    13201 catccgttct ggctcacaac gcattatgca gatggtgttt caggacccgc tctcttcgct
    13261 caatccgcgc ttaccggtgt ggcgcatcat cacagaaccg ctctggatag ctaagcgtag
    13321 tagtgaacaa cagcggcgag cgttggcaga ggagctggct gtgcaggtgg gtattcgtcc
    13381 ggagtatctc gaccgcctgc ctcatgcgtt ctccggcggg cagcggcaac gcatcgccat
    13441 tgccagagca ctctcttcgc agcctgacgt gattgtgctt gatgagccaa cctcggcgct
    13501 ggatatctcc gtgcaggcgc agatcctcaa tttactggta acgctacagg aaaatcacgg
    13561 gctgacttat gtgctgattt cacacaatgt ctcggtgata cgtcatatga gcgatcgggt
    13621 ggcggtgatg tatctcgggc agattgtgga actaggggaa gcgcaacagg tgttgactgt
    13681 tccggcacat ccatataccc gattattgct ggattccctc cccgccattg ataaaccgct
    13741 ggaggaagaa tgggcattac gtaaaacgga tctgccggga aaccgcacgt tgccgcaagg
    13801 ctgttttttc tacgaacgtt gccctctggc aacccacgga tgtgaagtcc ggcaatcatt
    13861 aacaataagg gaggacggac gtgagatccg gtgctggcgg gcgctgtaga gatgctaaca
    13921 acgcatcagg cattcacgcg cctgacgcgt catccggcaa tgctttacga tttcaactgg
    13981 taatcaagcg taatttccgc tttcagtacc tgagaaaccg ggcatcctgc ttttgctttc
    14041 tggatgatgc cgtcaaaggt agaggcatca atacccggca ccgcaacttc actcttcagt
    14101 gcgattttcg taatcgcaaa accggcatcc actttatcca gcgacacatc ggcggtggta
    14161 tcaatcgatg ttggcgtaaa tcccgcttcc cccagcatta atgaaagcgc cattgagaaa
    14221 catgcggcat gcgctgcgcc aatcagttct tcagggttag ttcctttttc gccttcaaaa
    14281 cgcgtgttaa atccatacgg ctgttggttc agcacaccac tctcggtgga tactgttccc
    14341 ttcccgcgtt tgatatcgcc ttcccagtgt gcctgacctt tcttatggat tgtcattgtt
    14401 gctctcctgt gggcttaaaa aggaaattat agactacgtg agaaacagca cgaggataag
    14461 caggatattc cgaataaaaa tccggcagaa atattacttc tgcacttaaa ttaaa
//
LOCUS       DAZKHF020000111        14427 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2201S31241
            SAMN43267800-rid25106413.denovo.112, whole genome shotgun sequence.
ACCESSION   DAZKHF020000111 DAZKHF020000000
VERSION     DAZKHF020000111.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267800
            Sequence Read Archive: SRR30307263
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 14427)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14427)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 14427)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350581.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350581.2
            Genome Coverage       :: 151x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:21:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,652
            CDSs (total)                      :: 5,557
            Genes (coding)                    :: 5,320
            CDSs (with protein)               :: 5,320
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 237
            CDSs (without protein)            :: 237
            Pseudo Genes (ambiguous residues) :: 0 of 237
            Pseudo Genes (frameshifted)       :: 102 of 237
            Pseudo Genes (incomplete)         :: 130 of 237
            Pseudo Genes (internal stop)      :: 52 of 237
            Pseudo Genes (multiple problems)  :: 41 of 237
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14427
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267800-rid25106413.denovo.112"
                     /serotype="O 26:H11"
                     /isolate="2201S31241"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-01"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            26..592
                     /locus_tag="ACHOSC_RS13370"
                     /old_locus_tag="pgaptmp_002674"
     CDS             26..592
                     /locus_tag="ACHOSC_RS13370"
                     /old_locus_tag="pgaptmp_002674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HGU4548589.1"
                     /translation="MFKGQKTLAALAVSLLFTAPVYAADEGSGEIHFKGEVIEAPCEI
                     HQDDIDKEVELGQVTTSHINQSHHSDAVAVDLRLVNCDLENSSNGSGGKISKVAVTFD
                     SSAKTTGADPILNNTSTGEATGVGVRLMNKDQSNIVLGTATPDIDLAPTSSEQTLNFF
                     AWMEQIDQATPVTPGAVTANATYVLDYK"
     gene            643..3102
                     /locus_tag="ACHOSC_RS13375"
                     /old_locus_tag="pgaptmp_002675"
     CDS             643..3102
                     /locus_tag="ACHOSC_RS13375"
                     /old_locus_tag="pgaptmp_002675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706516.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015473 - fimbrial usher porin activity
                     [Evidence IEA];
                     GO_process: GO:0009297 - pilus assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbria/pilus outer membrane usher protein"
                     /protein_id="HGU4548590.1"
                     /translation="MDTVNIYRLSFVSCLVVAMPCALAVEFNLNVLDKSMRDRIDISL
                     LKEKGVIAPGEYFVSVAVNNNQISNGQKINWHKNDDKTIPCINDLLVDKFGLKPEVRQ
                     SLPLINQCVDFSSRPEMLFNFDQANQQLNITIPQAWLAWHSENWTPPSTWKEGVAGVL
                     MDYNLFASSYRPQDGSSSTNLNAYGTAGINTGAWRLRSDYQLNQTDSDDNHEQSGEIS
                     RTYLFRPLPQLGSKLTLGETDFSSNIFDGFSYTGAALASDDRMLPWELRGYAPQISGI
                     AQTNATVTISQSGRVIYQKKVPPGPFIIDDLNQSVQGTLDVKVTEEDGRVNNFQVSAA
                     STPFLTRQGQVRYKLAAGQPRPSMSHQTENETFFSNDVSWGMLSNTSLYGGLLLSGDD
                     YHSAAIGIGQNMLWLGALSFDVTWASSHFDTQQDERGLSYRFNYSKQVDATNSTISLA
                     AYRFSDRHFHSYANYLDHKYNDSDAQDEKQTISLSVGQPITPLNLNLYANLLHQTWWN
                     ADASTTANITAGFNVDIGDWRDISISTSFNTTHYEDKDRDNQIYLSISLPFGNGGRVG
                     YDMQNSSHSTTHRMSWNDTLDERNSWGMSAGLQSDRPDNGAQVSGNYQHLSSAGEWDI
                     SGTYAANDYSSVSSSWSGSFTATQYGAAFHRRSSTNEPRLMVSTDGVADIPVQGNLDY
                     TNHFGIAVVPLISSYQPSTVAVNMNDLPDGVTVAENVIKETWIEGAIGYKSLASRSGK
                     DVNVIIRNASGQFPPLGADIRQDDSGISVGMVGEEGHAWLSGVAENQKFTVVWGDSQH
                     CSLHLPEHMEDTANRLILPCH"
     gene            3117..3848
                     /locus_tag="ACHOSC_RS13380"
                     /old_locus_tag="pgaptmp_002676"
     CDS             3117..3848
                     /locus_tag="ACHOSC_RS13380"
                     /old_locus_tag="pgaptmp_002676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706517.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0061077 - chaperone-mediated protein
                     folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone"
                     /protein_id="HGU4548591.1"
                     /translation="MTFMKGLPLLLLVASLCSHAALQPDRTRIVFNANDKATSLRVDN
                     RSDKLPYLAYSWLENEKGEKSDDLLVALPPIQRLEPKATTQVRIVKQASTTKLPGDRE
                     TLFFYNMREIPPAPEKNSDHAVLQVAIQSRIKVFWRPAALRKKAGEKVELQLQVSQQG
                     NQLTLKNPTAYYLTIAYLGRNEKGVLPGFKTVMVAPFSTVNTNIGNYSGSQFYLGYMD
                     DYGALRMTTLNYSGQCHLQAVEAKK"
     gene            3845..4906
                     /locus_tag="ACHOSC_RS13385"
                     /old_locus_tag="pgaptmp_002677"
     CDS             3845..4906
                     /locus_tag="ACHOSC_RS13385"
                     /old_locus_tag="pgaptmp_002677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415244.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HGU4548592.1"
                     /translation="MSAGKGVLLVICLLFLPLKSALALNCYFGSSGGSVEKSEAIQPF
                     AVPGNAKLGDKIWESDDIKIPVYCDNNTNGNFESEHVYAWVNPYPGVQDRYYQLGVTY
                     NGVDYDANQGKSRIDTNQCIDSKNIDIYTPEQIIAMGWQNKICSGDPANIHMSRTFLA
                     RMRLYVKIREMPPHDYQSTLSDYIVVQFDGAGSVNEDPTAQNLKYHITGLENIRVLDC
                     SVNFSISPETQVIDFGKFNLLDIRRHTMSKTFSIKTTKSQNDQCTDGFKVSSSFYTEE
                     TLVEEDKALLIGNGLKLRLLDENASPYTFNKYAEYADFTSDMLVYEKTYTAELSSIAG
                     TPIEAGPFDTVVLFKINYN"
     gene            5058..6104
                     /locus_tag="ACHOSC_RS13390"
                     /old_locus_tag="pgaptmp_002678"
     CDS             5058..6104
                     /locus_tag="ACHOSC_RS13390"
                     /old_locus_tag="pgaptmp_002678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415243.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB family transcriptional regulator"
                     /protein_id="HGU4548593.1"
                     /translation="MPVLQWGMLCVLSLLLSIGFLAVHLPAALLLGPMIAGIIFSMRG
                     ITLQLPRSAFLAAQAILGCMIAQNLTGSILTTLAANWPIVLAILLVTLLSSAIVGWLL
                     VRYSSLPGNTGAWGSSPGGAAAMVAMAQDYGADIRLVAFMQYLRVLFVAGAAVLVTRM
                     MLGDNAEAVNQQIVWFPPVSINLLLTILLAVVAGTAGCMLRLPSGTMLIPMLAGAVLQ
                     SGQLITIELPEWLLAMAYMAIGWRIGLGFDKQILLRALRPLPQILLSIFALLAICAGM
                     AWGLTRFMHIDFMTAYLATSPGGLDTVAVIAAGSNADMALIMAMQTLRLFSILLTGPA
                     IARFISTYAPKRSA"
     gene            complement(6101..6892)
                     /gene="nei"
                     /locus_tag="ACHOSC_RS13395"
                     /old_locus_tag="pgaptmp_002679"
     CDS             complement(6101..6892)
                     /gene="nei"
                     /locus_tag="ACHOSC_RS13395"
                     /old_locus_tag="pgaptmp_002679"
                     /EC_number="4.2.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000703 - oxidized pyrimidine nucleobase
                     lesion DNA N-glycosylase activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0140078 - class I DNA-(apurinic or
                     apyrimidinic site) endonuclease activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease VIII"
                     /protein_id="HGU4548594.1"
                     /translation="MPEGPEIRRAADNLEAAIKGKPLTDVWFAFPQLKTYQSQLIGQH
                     VTHVETRGKALLTHFSNELTLYSHNQLYGVWRVVDTGEEPQTTRVLRVKLQTADKTIL
                     LYSASDIEMLTPEQLTTHPFLQRVGPDVLDPNLTPEVVKERVLSPRFRNRQFAGLLLE
                     QAFLAGLGNYLRVEILWQVGLTGNHKAKDLNAAQLDALAHALLEIPRFSYATRGQVDE
                     NKHHGALFRFKVFHRDGEPCERCGSIIEKTTLSSRPFYWCPGCQH"
     gene            complement(6928..7662)
                     /gene="pxpA"
                     /locus_tag="ACHOSC_RS13400"
                     /old_locus_tag="pgaptmp_002680"
     CDS             complement(6928..7662)
                     /gene="pxpA"
                     /locus_tag="ACHOSC_RS13400"
                     /old_locus_tag="pgaptmp_002680"
                     /EC_number="3.5.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-oxoprolinase subunit PxpA"
                     /protein_id="HGU4548595.1"
                     /translation="MKIDLNADLGEGCASDAELLTLVSSANIACGFHAGDAQTMQACV
                     REAIKNGVAIGAHPSFPDRENFGRSAMQLPPETVYAQTLYQIGALATIARAQGGVMRH
                     VKPHGMLYNQAAKEAQLADAIARAVYACDPALILVGLAGSELIRAGKQYGLTTREEVF
                     ADRGYQADGSLVPRSQPGALIENEQQALAQTLEMVQHGRVKSITGEWATVAAQTVCLH
                     GDGEHALAFARRLRATFAKKGIVVAA"
     gene            complement(7652..8584)
                     /gene="pxpC"
                     /locus_tag="ACHOSC_RS13405"
                     /old_locus_tag="pgaptmp_002681"
     CDS             complement(7652..8584)
                     /gene="pxpC"
                     /locus_tag="ACHOSC_RS13405"
                     /old_locus_tag="pgaptmp_002681"
                     /EC_number="3.5.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-oxoprolinase subunit PxpC"
                     /protein_id="HGU4548596.1"
                     /translation="MLKIIRAGMYTTVQDGGRHGFRQSGISHCGALDMPALRIANLLV
                     GNDANAPALEITLGQLTVEFETDGWFALTGAGCEARLDDNAVWTGWRLPMKAGQRLTL
                     KRPQHGMRSYLAVAGGIDVPPVMGSCSTDLKVGSGGLEGRLLKDGDRLPIGKAKRDFM
                     EAQGVKQLLWGNRIRALPGPEYHEFDRASQDAFWRSPWQLSLQSNRMGYRLQGQILKR
                     TTDRELLSHGLLPGVVQVPHNGQPIVLMNDAQTTGGYPRIACIIEADMYHLAQIPLGQ
                     PIHFVQCSLEEALKARQDQQRYFEQLAWRLHNEN"
     gene            complement(8578..9234)
                     /gene="pxpB"
                     /locus_tag="ACHOSC_RS13410"
                     /old_locus_tag="pgaptmp_002682"
     CDS             complement(8578..9234)
                     /gene="pxpB"
                     /locus_tag="ACHOSC_RS13410"
                     /old_locus_tag="pgaptmp_002682"
                     /EC_number="3.5.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0017168 - 5-oxoprolinase (ATP-hydrolyzing)
                     activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-oxoprolinase subunit PxpB"
                     /protein_id="HGU4548597.1"
                     /translation="MQRARCYLIGETAVVLELEPPVTLASQKRIWRLAQRLVDMPNVV
                     EAIPGMNNITVILRNPESLALDAIERLQRWWEESEALEPESRFIEIPVVYGGAGGPDL
                     AVVAAHCGLSEKQVVELHSSVEYVVWFLGFQPGFPYLGSLPEQLHTPRRAEPRLLVPA
                     GSVGIGGPQTGVYPLATPGGWQLIGHTSLSLFDPARDEPILLRPGDSVRFVPQKEGVC
                     "
     gene            complement(9257..10000)
                     /gene="ybgI"
                     /locus_tag="ACHOSC_RS13415"
                     /old_locus_tag="pgaptmp_002683"
     CDS             complement(9257..10000)
                     /gene="ybgI"
                     /locus_tag="ACHOSC_RS13415"
                     /old_locus_tag="pgaptmp_002683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000798886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="radiation resistance protein YbgI"
                     /protein_id="HGU4548598.1"
                     /translation="MKNTELEQLINEKLNSAAISDYAPNGLQVEGKETVQKIVTGVTA
                     SQALLDEAVRLGADAVIVHHGYFWKGESPVIRGMKRNRLKTLLANDINLYGWHLPLDA
                     HPELGNNAQLAALLGITVMGEIEPLVPWGELTMPVPGLELASWIEARLGRKPLWCGDT
                     GPEVVQRVAWCTGGGQSFIDSAARFGVDAFITGEVSEQTIHSAREQGLHFYAAGHHAT
                     ERGGIRALSEWLNENTDLDVTFIDIPNPA"
     gene            10271..11752
                     /gene="dtpD"
                     /locus_tag="ACHOSC_RS13420"
                     /old_locus_tag="pgaptmp_002684"
     CDS             10271..11752
                     /gene="dtpD"
                     /locus_tag="ACHOSC_RS13420"
                     /old_locus_tag="pgaptmp_002684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide permease DtpD"
                     /protein_id="HGU4548599.1"
                     /translation="MNKHASQPRAIYYVVALQIWEYFSFYGMRALLILYLTNQLKYND
                     THAYELFSAYCSLVYVTPILGGFLADKVLGNRMAVMLGALLMAIGHVVLGASEIHPSF
                     LYLSLAIIVCGYGLFKSNVSCLLGELYEPTDPRRDGGFSLMYAAGNVGSIIAPIACGY
                     AQEEYSWAMGFGLAAVGMIAGLVIFLCGNRHFTHTRGVNKKVLRATNFLLPNWGWLLV
                     LLVATPALITVLFWKEWSVYALIVATIIGLGVLAKIYRKAENQKQRKELGLIVTLTFF
                     SMLFWAFAQQGGSSISLYIDRFVNRDMFGYTVPTAMFQSINAFAVMLCGVFLAWVVKE
                     SVAGNRTVRIWGKFALGLGLMSAGFCILTLSARWSAMYGHSSLPLMVLGLAVMGFAEL
                     FIDPVAMSQITRIEIPGVTGVLTGIYMLLSGAIANYLAGVIADQTSQASFDASGAINY
                     SINAYIEVFDQITWGALACVGLVLMIWLYQALKFRNRALALES"
     gene            complement(11794..13212)
                     /gene="phrB"
                     /locus_tag="ACHOSC_RS13425"
                     /old_locus_tag="pgaptmp_002685"
     CDS             complement(11794..13212)
                     /gene="phrB"
                     /locus_tag="ACHOSC_RS13425"
                     /old_locus_tag="pgaptmp_002685"
                     /EC_number="4.1.99.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001740284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribodipyrimidine photo-lyase"
                     /protein_id="HGU4548600.1"
                     /translation="MTTHLVWFRQDLRLHDNLALAAACRNSSARVLALYIATPRQWAT
                     HNMSPRQAELINAQLNGLQIALAEKGIPLLFREVDDFVASVEIVKQVCAENSVTHLFY
                     NYQYEVNERARDVEVERALRNVVCEGFDDSVILPPGAVKTGNHEMYKVFTPFKNAWLK
                     RLREGMPECVAAPKVRSSGSIEPSPSITLNYPRQSFDTAHFPVEEKAAIAQLRQFCQN
                     GAGEYEQQRDFPAVEGTSRLSASLATGGLSPRQCLHRLLAEQPQALDGGAGSVWLSEL
                     IWREFYRHLMTYYPSLCKHCPFIAWTDRVQWQSNPAHLQAWQEGKTGYPIVDAAMRQL
                     NSTGWMHNRLRMISASFLVKDLLIDWREGERYFMSQLIDGDLAANNGGWQWAASTGTD
                     AAPYFRIFNPTTQGEKFDREGEFIRRWLPELRDVPGKAVHEPWKWAQKAGVMLDYPQP
                     IVDHKEARLRTLAAYEEARKGA"
     gene            complement(13209..13718)
                     /locus_tag="ACHOSC_RS13430"
                     /old_locus_tag="pgaptmp_002686"
     CDS             complement(13209..13718)
                     /locus_tag="ACHOSC_RS13430"
                     /old_locus_tag="pgaptmp_002686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbgA family protein"
                     /protein_id="HGU4548601.1"
                     /translation="MNQQRFDDSTLIRIFALHELHRLKEHGLTRGALLDYHSRYKLVF
                     LAHSQPEYRKLGPFVADIHQWQNLDDFYNQYYQRVIVLLSHPANPRDHTNVLMHVQGY
                     FRPHIDSTERQQLAALIDSYRRGEQPLLAPLMRIKHYMALYPDAWLSGQRYFELWPRV
                     INLRHSGVL"
     gene            complement(13869..14427)
                     /locus_tag="ACHOSC_RS13435"
                     /old_locus_tag="pgaptmp_002687"
                     /pseudo
     CDS             complement(13869..14427)
                     /locus_tag="ACHOSC_RS13435"
                     /old_locus_tag="pgaptmp_002687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000420980.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ISAs1 family transposase"
BASE COUNT         3473 a         3855 c         3507 g         3592 t
ORIGIN      
        1 ttcttttctg ttaaaggact ccgttatgtt taaaggacaa aaaacattgg ccgcactggc
       61 cgtatctctg ctgttcacag cacctgttta tgcggctgat gaaggatccg gtgaaattca
      121 ctttaaaggt gaagttattg aagcaccgtg tgaaatacat caggatgata ttgataaaga
      181 ggttgaactc ggtcaggtga ccaccagcca cattaatcag tcacatcaca gcgatgccgt
      241 tgctgtcgac ctgcgcttag tcaactgtga tctggaaaac tccagcaacg gttccggtgg
      301 caagatttct aaagttgcag tcacctttga tagctcagcg aaaaccaccg gcgcagatcc
      361 gattctcaac aacaccagca cgggtgaagc caccggcgtc ggcgtacgtt taatgaataa
      421 agatcaaagc aatatcgttt taggcaccgc tactccagat atcgacctgg ctccgacctc
      481 cagcgaacaa acgctgaatt tctttgcctg gatggaacaa attgatcagg ctacacctgt
      541 aacgccaggc gcagttacgg cgaatgcaac ctacgtgctg gattataaat aagtattact
      601 ttggcggcgc tatcgccgcc tttcataaaa tatttatcag gtatggacac agtgaatatt
      661 tatcgactct cttttgtatc ctgcctggtc gtggcgatgc cttgcgcatt ggcggtcgaa
      721 ttcaacctta atgttctcga taaatcaatg cgcgaccgca ttgatatttc attattaaag
      781 gaaaaaggag tcattgctcc cggtgagtat tttgttagcg ttgcggtaaa taataaccaa
      841 atcagtaacg ggcaaaagat taactggcac aaaaatgacg ataaaaccat tccgtgcatc
      901 aatgatttac tggtcgataa atttggctta aaacctgaag tccgtcagtc gttaccattg
      961 ataaatcagt gcgtcgattt tagctcccga cctgaaatgc tcttcaattt cgatcaagcc
     1021 aatcagcaac taaatatcac cattccgcaa gcctggctgg cgtggcactc agaaaactgg
     1081 accccaccct ccacatggaa agaaggtgtc gccggtgtcc tgatggatta caacttgttt
     1141 gccagcagct accgcccaca ggatggcagc agcagcacta atcttaacgc ctatggaaca
     1201 gccggaatca acacgggggc atggcgctta cgcagtgatt atcagttgaa tcagactgat
     1261 agcgatgata accatgaaca gtcaggcgaa atatcacgta cctatctttt tcgcccgtta
     1321 ccgcaattag gttcaaaatt aaccctcggc gaaacggatt ttagttccaa tattttcgac
     1381 ggtttttctt ataccggcgc ggcactggca agtgacgatc gaatgttgcc atgggaacta
     1441 cgcggctacg ccccacaaat tagcggtatt gcacagacca atgccacggt gacgatcagt
     1501 caatcaggcc gcgtcattta ccagaaaaaa gtcccaccag gcccatttat cattgacgac
     1561 cttaatcagt ctgttcaggg cacactggat gtcaaagtga cggaagaaga tggtcgggtg
     1621 aacaatttcc aggtttcggc agcatcgacg cccttcctga ctcgtcaggg acaggttcgc
     1681 tataaactgg ccgcgggtca gccacggccc tccatgtcac atcaaactga aaatgaaacc
     1741 ttttttagca atgatgtttc ctgggggatg ctgtcaaaca cctcgctgta cggcggcctg
     1801 ctgctttctg gtgatgacta ccattctgcc gcaataggta tcgggcaaaa tatgctgtgg
     1861 cttggcgcgc tgtcgtttga tgtcacgtgg gccagtagtc actttgatac tcagcaggac
     1921 gagcggggct taagctaccg ttttaattac agcaaacaag tggatgccac caacagcacg
     1981 atttccctcg ccgcttatcg tttctccgat cgtcattttc acagctacgc caactatctt
     2041 gatcacaaat acaacgacag cgatgcgcag gacgaaaaac agacgatcag cttatctgtg
     2101 ggccaaccga ttaccccact aaacctcaat ctttacgcca acctgctaca tcaaacctgg
     2161 tggaatgcag acgcctccac gaccgccaac atcacggcag gttttaatgt tgatattggt
     2221 gactggagag atatctctat ttcgacgtca ttcaatacaa cccattacga agataaagat
     2281 cgcgacaacc agatttacct gtcgatttcg ctccccttcg gtaacggtgg tcgggttggc
     2341 tatgacatgc aaaacagtag ccacagcacc acacaccgca tgtcgtggaa cgatacgctg
     2401 gatgaacgta atagctgggg catgtctgcc ggactgcaat ccgaccgtcc ggacaatgga
     2461 gcccaggtga gcggtaacta tcagcatctg agttcagcgg gtgagtggga tatttctggt
     2521 acctatgccg ccaatgatta cagttccgtc agcagcagct ggagcggttc tttcaccgca
     2581 acccaatatg gtgcagcgtt tcatcgccgc agctccacca atgaaccacg cctgatggtc
     2641 agcaccgatg gcgtggcaga tattccggtt cagggcaatc tcgactacac caaccatttt
     2701 ggcattgcgg tggtgccgtt gatttccagt taccagcctt ccaccgtggc ggtgaacatg
     2761 aatgacttac ccgacggcgt aacagttgca gaaaacgtta tcaaagaaac gtggattgaa
     2821 ggcgcgatag gttacaaatc actggcttcc cgttccggta aagacgttaa cgtcatcatt
     2881 cgcaacgcca gcggtcagtt tcctcccctc ggagcggata tccgccagga tgacagcggc
     2941 attagcgtgg ggatggttgg cgaggaagga catgcctggt taagcggagt cgctgaaaat
     3001 caaaagttta ccgtggtctg gggtgatagc cagcattgct cgctccatct tcctgaacat
     3061 atggaagaca ccgcaaatcg cctgatttta ccttgtcatt aattaaggaa atgaaaatga
     3121 catttatgaa aggactaccc cttttgttgt tggttgccag tctgtgcagc cacgctgcac
     3181 tacaacccga tcgcactcgt attgtgttta acgctaatga taaagctacc agtctgcggg
     3241 tggataatcg tagcgataaa ctcccctacc tcgcttattc ctggctcgaa aatgaaaaag
     3301 gggagaaaag cgatgatctt ctggtcgccc tgccgccgat acaacgtctg gaaccgaaag
     3361 caacaacgca ggtacggata gtaaagcaag cctcgaccac aaaattaccg ggcgatcgcg
     3421 aaacgctatt tttctacaat atgcgtgaaa ttcctcccgc cccggaaaaa aacagcgacc
     3481 atgccgttct gcaagtcgcc attcagagtc gtatcaaggt gttctggcga ccggctgcat
     3541 tacgcaagaa agcaggagaa aaggtcgaac tgcagttaca ggtcagccag cagggcaacc
     3601 aactgacact gaaaaatcct accgcgtatt atctgaccat tgcctacctg ggacgaaatg
     3661 aaaaaggcgt tctccccggc ttcaaaaccg tgatggttgc gccttttagt acggtcaaca
     3721 caaacatcgg aaattatagc ggcagccagt tttatctcgg ttatatggat gattacggcg
     3781 cgttacgcat gacgacgctc aactacagcg gacaatgcca tttacaagcc gtggaggcga
     3841 agaaatgagt gctggcaagg gagtgttgct cgtcatctgt ctgttatttc tgccgttgaa
     3901 gtctgcgctt gccctgaact gctactttgg ttcatccggt ggctcggtag aaaaatcaga
     3961 agcgattcaa ccttttgctg taccaggcaa tgccaaactc ggcgataaga tttgggaatc
     4021 tgacgatatt aaaattcccg tctattgtga caacaatacc aacggcaatt ttgaaagtga
     4081 gcatgtttat gcctgggtaa acccctaccc gggggtgcag gatcgctatt atcaactcgg
     4141 cgtaacttac aatggcgttg attatgacgc caaccaggga aaaagccgca tcgacactaa
     4201 ccagtgtatt gacagtaaga acattgacat ttatacccct gagcagatta tcgcgatggg
     4261 atggcagaac aaaatttgct cgggtgatcc cgctaatatt cacatgtcac gtacctttct
     4321 cgctcgcatg cggttatacg tcaaaatacg agaaatgccg ccgcatgatt atcaaagtac
     4381 gcttagcgac tatatcgtcg tgcaatttga cggtgccggt agcgttaacg aagatcctac
     4441 tgcccaaaac ctgaaatatc atattaccgg tctggaaaac attcgcgtgc tggattgcag
     4501 cgtcaatttt tccatttcac cggaaacaca ggtgattgat tttggtaaat ttaatttgct
     4561 ggatattcgt cgccacacaa tgtcgaaaac attcagtatt aagacgacaa aatcacaaaa
     4621 tgaccaatgc accgacggat ttaaagtcag ttcctccttt tataccgaag aaacgttggt
     4681 tgaagaagat aaagcattgt tgattggtaa tggtctgaag ctacgtttgc tggatgaaaa
     4741 cgcctcgcct tataccttca ataaatatgc tgaatacgcc gatttcacca gcgacatgtt
     4801 ggtctacgaa aaaacctata cggctgaact ttcgtccatt gcaggtaccc ccatcgaggc
     4861 tgggcccttc gatacagtgg tgctttttaa aattaactat aactgatttg ccatgatgcc
     4921 ctttgcagcc ggaaagggca tctctttcag aaaattgctg attttcccct tttccttgat
     4981 tgctgttatt ttttcctggc ccggctttct ttatgctgta tcgatacacc aaagagaaaa
     5041 taatgaggga gcgaaggatg ccagttttgc agtggggaat gttatgtgtg ctatcacttc
     5101 tcctttctat tggtttcctc gcggtacatc tcccggcggc gctattactt gggccgatga
     5161 tcgccgggat catcttcagt atgcgcggaa ttactctgca acttccccgc tccgcttttc
     5221 tcgccgcgca ggccattctt ggctgcatga ttgcgcaaaa cctcactggt tcgattctca
     5281 ccacgctggc cgccaactgg ccgatcgtgc ttgctatttt actggtgacg ctgctttcca
     5341 gcgccatcgt gggttggtta ttggtgcgct atagctcact gcctggaaat accggtgcct
     5401 ggggttcttc tccaggcggc gcagcggcaa tggtcgctat ggcgcaagat tacggcgcag
     5461 atattcgcct ggtggcgttt atgcaatatc tgcgagtgct gttcgtcgcg ggggccgctg
     5521 ttctggtcac ccgcatgatg ctgggtgata acgctgaagc ggttaaccag caaatcgtct
     5581 ggttcccgcc agtgagcatt aatctcctgc ttacgatttt gctcgcagtc gttgccggta
     5641 cggcgggatg catgctgcgt cttccttccg gcacgatgct catcccgatg ctggcgggcg
     5701 cagtgctcca gtctggtcag ctcatcacca tcgaactccc ggaatggctg cttgcgatgg
     5761 cgtatatggc aattggctgg cggattggtc ttggtttcga taaacaaatc ttactgcggg
     5821 cactacgccc gctaccgcaa atcctgctgt cgatttttgc tctgttggct atttgtgcgg
     5881 gtatggcgtg ggggctgacc cggtttatgc atattgattt tatgactgcc tacctcgcca
     5941 ccagccccgg cgggcttgat accgtggcgg tcatcgccgc agggagcaat gccgatatgg
     6001 cgcttattat ggcgatgcaa accctgcgcc tgttcagtat tttgctgacg ggacctgcca
     6061 ttgcacggtt tatttcaacc tatgcgccga agcggtcggc ttagtgctgg cagccagggc
     6121 accagtaaaa cgggcgagat gacagcgtgg ttttctcaat gatgctgcca caacgttcgc
     6181 acggttcgcc atctcgatga aaaaccttaa agcgaaacag cgccccatga tgcttattct
     6241 catccacctg cccccgcgta gcgtaagaaa atcgaggaat ctccagtaac gcgtgtgcga
     6301 gtgcatccag ttgcgccgca ttgagatctt tcgctttatg atttccagtc aacccaacct
     6361 gccagaggat ctccacccgc aaataattgc caagtcctgc cagaaacgcc tgctcgagca
     6421 gtaatcctgc aaactgacgg ttacgaaagc gcggcgacaa tacccgttct ttcaccacct
     6481 ccggcgtcag attcggatcc agcacatcgg gaccaacgcg ttgtaaaaac ggatgcgtgg
     6541 tcagttgttc cggggtcaac atctcaatat ccgaggcgct ataaagcaga atggttttgt
     6601 cagccgtttg cagttttacc cgcaatactc gcgtggtctg cggctcttcg ccggtatcaa
     6661 ccacgcgcca gacgccgtaa agctgattat ggctgtagag cgttaattcg ttggaaaaat
     6721 gagttaacaa cgccttacca cgcgtttcca catgggtaac gtgttgaccg ataagttgtg
     6781 attgataagt ttttaactgc gggaaggcaa accagacatc agttagtggt ttgcctttga
     6841 tcgccgcctc caggttatcc gctgcacggc ggatctccgg gccttcaggc atggtgatat
     6901 ccttttttca ttctttatta agcggggtta tgctgcgaca acaatcccct ttttggcaaa
     6961 tgtagctcgt aatcggcggg cgaacgccag agcgtgctcg ccgtcgccat gcaggcagac
     7021 cgtttgcgcc gcgaccgttg cccattcgcc ggtgatactt ttgactctgc cgtgttgcac
     7081 catctccagc gtttgcgcca gcgcctgttg ttcgttttca atcaacgcgc ctggctgact
     7141 tcgcggcacc agcgagccgt cagcctgata accgcgatcg gcaaacactt cctcgcgcgt
     7201 tgtcaggcca tattgcttgc ctgcacgaat cagctcgctt cctgccagcc caacgagaat
     7261 caatgccgga tcgcaagcgt ataccgctct ggcgatggcg tctgccagtt gtgcttcttt
     7321 cgccgcctgg ttgtacaaca tgccgtgcgg tttgacatga cgcattacgc cgccttgcgc
     7381 acgggcaatc gttgccagcg cgccaatttg atacagcgtc tgggcgtaaa cggtttctgg
     7441 cggcaactgc atggcgctgc gaccaaaatt ttccctgtcg ggaaaactcg ggtgagcgcc
     7501 aattgcaaca ccatttttta ttgcttcacg cacgcaagcc tgcatggttt gcgcatcgcc
     7561 tgcatgaaat ccacaggcaa tattggcaga ggaaaccagc gttaatagct ctgcgtcgct
     7621 ggcgcagcct tcgcccagat cggcgttcag gtcaattttc attgtgcagc cgccacgcta
     7681 attgttcaaa ataacgttgc tgatcttgcc gcgctttcag tgcctcttcc agtgaacact
     7741 ggacaaaatg aatcggctga ccgagcggaa tttgcgcaag atggtacata tcagcctcaa
     7801 tgatgcaggc aatacgcggg taaccaccgg tggtctgtgc gtcgttcatc aacacaatcg
     7861 gctgcccgtt atgtggcacc tgcaccacgc ccggtaacaa accgtgagat aacagttcgc
     7921 gatcggtggt gcgttttaaa atttgcccct gtaagcgata gcccatgcgg ttactttgca
     7981 agctaagctg ccagggcgaa cgccagaatg catcctgcga ggcgcgatcg aactcatgat
     8041 attccggccc cggcaaggcg cgaatgcggt tgccccacag cagctgttta acgccctgcg
     8101 cttccataaa atcacgcttc gctttgccaa tcgggagtcg gtcaccatcc ttcagtaaac
     8161 ggccttccag cccgccactc cccactttga gatcggtgct gcatgacccc attaccggcg
     8221 gaacatcaat accacccgcg accgccagat aactgcgcat cccgtgctgc gggcgtttaa
     8281 gcgttaaacg ctggcctgct ttcatcggca atcgccagcc ggtccagacg gcattatcat
     8341 ccagccgcgc ttcgcaaccg gcacccgtca gagcaaacca cccatcagtt tcgaactcaa
     8401 cagttaactg accgagcgtg atctccagcg cgggggcatt ggcgtcatta cccaccagta
     8461 ggttagcaat gcgtaacgcg ggcatatcca gtgcgccgca gtggctgata cccgactggc
     8521 gaaaaccgtg acgaccgcca tcctgcacag tggtatacat gcccgcacga ataatcttca
     8581 gcatactccc tccttctgcg gtacaaagcg cacgctgtct cccggacgta ataagatggg
     8641 ttcgtcacgc gccggatcaa acaggctgag tgaggtatga ccaatcaact gccagccacc
     8701 cggcgttgcc agcggataaa caccagtctg cggcccgccg atcccgacag aacctgccgg
     8761 aacgagtaag cgcggttcag cgcgccgtgg cgtgtgtagt tgttccggca aactcccgag
     8821 atacgggaag cccggttgaa aacctaaaaa ccagaccacg tattccacgg aggagtgcaa
     8881 ttcaacaacc tgtttttcgc tcaacccgca atgcgccgcg accaccgcca aatccggtcc
     8941 gcctgcacca ccgtaaacca ccggaatttc aataaagcga gactcaggtt ccagggcctc
     9001 gctctcctcc caccagcgtt gcaaacgctc tatggcatcc agcgccagcg actcaggatt
     9061 acgcaaaatc accgtaatat tgttcatgcc gggaatggct tcaaccacat tcggcatatc
     9121 caccagacgc tgcgccagtc gccagatccg tttctggcta gccagcgtca ccggcggttc
     9181 cagttccagc actaccgccg tttcacctat cagataacaa cgcgctcgtt gcactttcga
     9241 tccctctgat tattcgttat gcaggattag gaatatcaat aaaggtcaca tcaagatcgg
     9301 tattttcatt cagccactcg ctcaatgcgc gaataccacc acgttcagtg gcatggtgac
     9361 ctgcagcata aaaatgcaat ccctgctcgc gggctgaatg aatggtctgt tcagaaactt
     9421 cgccagtaat aaaagcatcc acgccaaaac gcgcggcgct atcgataaaa ctttgcccgc
     9481 cgcccgtgca ccaggcgacg cgctggacca cctcaggtcc ggtatcgcca caccataacg
     9541 gcttacgtcc cagacgcgct tcaatccagg aggctagttc cagtcccggc acaggcatgg
     9601 tcagctctcc ccacggcacc aacggctcaa tttcgcccat gaccgtgatc cccagtaacg
     9661 ccgccagctg tgcgttattg cccagctcag gatgtgcgtc aagcggcaaa tgccagccat
     9721 acaggttgat atcattcgcc agcaacgttt ttaaacggtt acgcttcatt ccgcgaatga
     9781 ccggagactc tcctttccag aagtagccat gatgcacaat gaccgcatcc gcgcccagac
     9841 gcactgcctc atcgagcaaa gcctggctgg cggtgacacc ggtaacaatt ttttgcaccg
     9901 tctctttgcc ttccacctgc aaaccattcg gcgcgtaatc actaatcgcc gcgctgttca
     9961 gtttttcgtt aatcagttgt tccagttcgg tgtttttcat cttcgcgcct tccgtattct
    10021 gatagctaca ccgttaacat agcgttacct cgcctgttcg tcgatagcca atcttttgac
    10081 atattaagaa acaaacccga cgccaaaaga cgcaaattgt ttatatcatc acgcttcagc
    10141 taatgtatat ttatgcaatc agacagcata ttgcataaat aatcagatct gtatcaaagt
    10201 tttaacttta aggaaacaca agcacgccgc gcaactatta cgtgacgttg ccgtcaattg
    10261 tggaattatt atgaataaac acgcatcaca accgcgcgct atttactatg tcgttgcgct
    10321 gcaaatctgg gaatatttta gcttttacgg catgcgtgcc ctgctgattc tctatctcac
    10381 caatcaacta aaatacaacg atactcacgc ctacgagtta tttagcgcct actgttcgct
    10441 ggtgtatgtc acgccaatcc tcggtggctt tttggcggat aaagttctcg gcaatcgcat
    10501 ggcggtgatg ctgggggcgt tgttgatggc gatcggtcat gtggtgctgg gtgccagtga
    10561 gatccatccg tcattcctct atctgtccct ggcgattatc gtttgtggct atggtctgtt
    10621 taaatccaat gtcagttgcc tgctcggcga gctgtatgag ccaaccgatc cgcgtcgtga
    10681 tggcggtttc tcgctgatgt atgcagcggg taacgtgggg tctattatcg cacctatcgc
    10741 ctgtggttac gcccaggaag agtacagctg ggcgatgggc tttggcctgg cggcggtggg
    10801 tatgatcgcg ggtctggtca ttttcttatg tggcaatcgt catttcacac atacccgcgg
    10861 cgttaataaa aaagtactgc gtgcaacaaa ctttctcctg cctaactggg gatggctgct
    10921 ggttctgctg gtggcaacgc ctgcgctgat taccgtactg ttctggaaag agtggtcggt
    10981 atacgcctta attgtcgcga ctattattgg cctgggtgta ctggcaaaaa tttatcgcaa
    11041 agcagaaaac cagaaacagc gtaaggagct ggggctgatt gtgacgctca ccttcttcag
    11101 tatgttgttc tgggccttcg cacaacaggg cggcagctcg attagccttt atatcgaccg
    11161 cttcgttaac cgcgatatgt ttggttatac cgttccgact gctatgttcc agtcgattaa
    11221 tgccttcgca gttatgctgt gcggtgtgtt cctggcgtgg gtggttaaag aaagcgtcgc
    11281 aggtaatcgt accgtgcgca tctggggaaa atttgctctt ggccttggcc tgatgagcgc
    11341 cggattctgc attctgacct taagcgcccg ctggtccgca atgtatggtc actcttctct
    11401 gccactgatg gtattaggcc tggcggtgat gggctttgcg gaactgttta tcgacccggt
    11461 tgccatgtcg caaattacgc gcattgaaat tccgggcgtg accggcgtat taaccggcat
    11521 ctacatgctg ctttctggcg cgatcgcgaa ctatctggcg ggcgtgattg ccgatcagac
    11581 atcgcaggct tcgtttgatg cttccggggc gatcaactac tccatcaatg catatattga
    11641 agtatttgat caaattacct ggggcgcact ggcgtgtgta ggactggtac tgatgatttg
    11701 gctgtatcag gcgctgaaat tcagaaaccg cgcgctggcg ctggagtcat aataggccag
    11761 caccatacct gataaaacgc tcgctgcgtt ttatcaggct cctttcctcg cttcttcgta
    11821 cgctgccagc gttcgcagcc ttgcctcttt gtgatcaact atcggttgcg gataatccag
    11881 catcacacct gctttctgcg cccacttcca cggctcatgc accgctttcc ctggtacatc
    11941 gcgcagctcc ggtaaccatc ggcggataaa ctcaccttcg cgatcaaatt tctcgccctg
    12001 ggttgtcggg ttgaaaatac gaaaatacgg cgctgcatca gtacccgtag acgccgccca
    12061 ctgccagcca ccgttattgg ctgccaaatc accatcaatc agctgcgaca tgaaatatcg
    12121 ctcgccttcg cgccagtcga tcaacaaatc tttcaccaga aaactggctg aaatcatccg
    12181 tagccggtta tgcatccagc cagtgctgtt aagctgacgc atggcggcat cgacaatcgg
    12241 gtatcccgtt ttgccttcct gccaggcctg taaatgtgcg ggattgctct gccactgcac
    12301 acgatccgtc caggcaataa acggacaatg tttacacaac gaggggtaat acgtcatcag
    12361 atgacggtag aactcgcgcc agatcagctc actaagccag acactaccgg ccccaccgtc
    12421 cagcgcctgc ggctgttccg ccaacaagcg atgcaaacac tggcgaggcg ataacccgcc
    12481 cgttgccagg ctggcggaca aacgactggt gccttccact gccggaaaat ctcgttgttg
    12541 ctcatattct ccggcaccgt tctggcaaaa ctggcgtaat tgcgcaatcg ccgctttttc
    12601 ttccaccggg aaatgcgcag tatcgaaaga ctgacgagga taattcagcg taatggatgg
    12661 cgagggctct atcgatccgc tactacgaac ttttggcgca gcgacgcact ccggcatccc
    12721 ttcccgcagc cgtttcagcc aggcattctt aaaaggcgta aagactttgt acatctcgtg
    12781 attaccggtc ttcaccgcgc caggcggcag gatcacgctg tcatcaaatc cttcacacac
    12841 cacgttacgc agcgctcttt caacttccac atcccgcgcc cgctcattca cttcatactg
    12901 atagttataa aacaggtggg taacgctgtt ttccgcgcac acctgtttaa ctatttcgac
    12961 actggcgaca aagtcatcca cttcacggaa caataaagga ataccttttt ccgcaagcgc
    13021 tatttgtagc ccattcagtt gagcattgat aagttcagcc tgacgcggcg acatgttatg
    13081 cgtcgcccac tggcgtggtg tagcgatata caacgccagc acgcgtgcag acgaattgcg
    13141 gcaggcggca gccagtgcga gattatcgtg cagacgtaaa tcctggcgaa accagaccag
    13201 atgggtagtc ataaaactcc tgaatggcgc aagttaatca cacgcggcca aagttcgaaa
    13261 taacgctgcc ctgaaagcca ggcgtcagga taaagcgcca tatagtgttt gatacgcatc
    13321 agcggcgcaa gaagtggttg ctcgccacgg cgataactgt cgataagcgc cgccaactgc
    13381 tggcgttctg tggaatcaat atgcgggcga aaataaccct gaacgtgcat caaaacgttg
    13441 gtgtgatcgc gcgggttggc gggatgagaa agcaaaacaa ttacgcgttg gtagtactgg
    13501 ttgtaaaaat catccagatt ttgccactgg tgaatatcag ccacgaacgg gccaagtttg
    13561 cggtactccg gctgagaatg cgccagaaag acgagtttat agcggctgtg ataatcgaga
    13621 agcgccccgc gcgttaagcc atgttctttc agtcgatgta actcatgaag ggcgaagata
    13681 cggattaggg tgctgtcatc aaatcgttgc tgattcatta ctgctcctga acggtttaca
    13741 ggagtaagtg tagacgttga gaaatgatat ttgtgaagtt aatcctgttg tagcaggaca
    13801 aatttacgaa agcctgctcc ccgcaaggac tgacgtcagg tagtttctaa ccatagctgc
    13861 cctttctatt caaacgtgaa atcaataagc tcatcaggga catcgcaaac aatatgaaga
    13921 cgggtttctt ctctgccgtg actcttttca ctaattgcgt aactgtcatg cttgggatta
    13981 tttaattctt tcagcggaaa tttttcctcg aaggctttat ttagccgccc ctggtttcct
    14041 tttacagcga ataaataatc acctccctgt ttttgtatct tctctgcaat atctttctgg
    14101 caacccatcg catcggttgt gatgattttt cctttaatat ccagcatgtt aagaagttct
    14161 gggatagctg tgatctcatt ggatttctta tccgtcttga tctgcccgat gaccagactg
    14221 tgcattgttg agaacgcact aatgacatga atcgctcccc tgcggcgact cttgtcataa
    14281 gagtgccgga gcgtttttcc atcaattgca atgacgtctt tatcatttga tgaatggcaa
    14341 tcacgcatcc agttaataaa gcactcgtga aattttgcag gactgataca ggatacaact
    14401 ctggcaatag tatcgtgaac aggaata
//
LOCUS       DAZKHF020000112        14240 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2201S31241
            SAMN43267800-rid25106413.denovo.113, whole genome shotgun sequence.
ACCESSION   DAZKHF020000112 DAZKHF020000000
VERSION     DAZKHF020000112.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267800
            Sequence Read Archive: SRR30307263
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 14240)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14240)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 14240)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350581.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350581.2
            Genome Coverage       :: 151x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:21:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,652
            CDSs (total)                      :: 5,557
            Genes (coding)                    :: 5,320
            CDSs (with protein)               :: 5,320
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 237
            CDSs (without protein)            :: 237
            Pseudo Genes (ambiguous residues) :: 0 of 237
            Pseudo Genes (frameshifted)       :: 102 of 237
            Pseudo Genes (incomplete)         :: 130 of 237
            Pseudo Genes (internal stop)      :: 52 of 237
            Pseudo Genes (multiple problems)  :: 41 of 237
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14240
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267800-rid25106413.denovo.113"
                     /serotype="O 26:H11"
                     /isolate="2201S31241"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-01"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            complement(63..143)
                     /gene="shoB"
                     /locus_tag="ACHOSC_RS13440"
                     /old_locus_tag="pgaptmp_002688"
     CDS             complement(63..143)
                     /gene="shoB"
                     /locus_tag="ACHOSC_RS13440"
                     /old_locus_tag="pgaptmp_002688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin ShoB"
                     /protein_id="HGU4548602.1"
                     /translation="MTDCRYLIKRVIKIIIAVLQLILLFL"
     gene            complement(563..943)
                     /gene="acpS"
                     /locus_tag="ACHOSC_RS13445"
                     /old_locus_tag="pgaptmp_002689"
     CDS             complement(563..943)
                     /gene="acpS"
                     /locus_tag="ACHOSC_RS13445"
                     /old_locus_tag="pgaptmp_002689"
                     /EC_number="2.7.8.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008897 - holo-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="holo-ACP synthase"
                     /protein_id="HGU4548603.1"
                     /translation="MAILGLGTDIVEIARIEAVIARSGERLARRVLSDNEWAIWKTHH
                     QPVRFLAKRFAVKEAAAKAFGTGIRNGLAFNQFEVFNDELGKPRLRLWGEALKLAEKL
                     GVVNMHVTLADERHYACATVIIES"
     gene            complement(943..1674)
                     /gene="pdxJ"
                     /locus_tag="ACHOSC_RS13450"
                     /old_locus_tag="pgaptmp_002690"
     CDS             complement(943..1674)
                     /gene="pdxJ"
                     /locus_tag="ACHOSC_RS13450"
                     /old_locus_tag="pgaptmp_002690"
                     /EC_number="2.6.99.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033856 - pyridoxine 5'-phosphate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxine 5'-phosphate synthase"
                     /protein_id="HGU4548604.1"
                     /translation="MAELLLGVNIDHIATLRNARGTAYPDPVQAAFIAEQAGADGITV
                     HLREDRRHITDRDVRILRQTLDTRMNLEMAVTEEMLAIAVETKPHFCCLVPEKRQEVT
                     TEGGLDVAGQRDKMRDACKRLADAGIQVSLFIDADEEQIKAAAEVGAPFIEIHTGCYA
                     DAKTDAEQAQELARIAKAATFAASLGLKVNAGHGLTYHNVKAIAAIPEMHELNIGHAI
                     IGRAVMTGLKDAVAEMKRLMLEARG"
     gene            complement(1686..2414)
                     /gene="recO"
                     /locus_tag="ACHOSC_RS13455"
                     /old_locus_tag="pgaptmp_002691"
     CDS             complement(1686..2414)
                     /gene="recO"
                     /locus_tag="ACHOSC_RS13455"
                     /old_locus_tag="pgaptmp_002691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecO"
                     /protein_id="HGU4548605.1"
                     /translation="MEGWQRAFVLHSRPWSETSLMLDVFTEESGRVRLVAKGARSKRS
                     TLKGALQPFTPLLLRFGGRGEVKTLRSAEAVSLALPLSGITLYSGLYINELLSRVLEY
                     ETRFSELFFDYLHCIQSLAGATGTPEPALRRFELALLGHLGYGVNFTHCAGSGEPVDD
                     TMTYRYREEKGFIASVVIDNKTFTGRQLKALNAREFPDADTLRAAKRFTRMALKPYLG
                     GKPLKSRELFRQFMPKRTVKTHYE"
     gene            complement(2426..3331)
                     /gene="era"
                     /locus_tag="ACHOSC_RS13460"
                     /old_locus_tag="pgaptmp_002692"
     CDS             complement(2426..3331)
                     /gene="era"
                     /locus_tag="ACHOSC_RS13460"
                     /old_locus_tag="pgaptmp_002692"
                     /EC_number="3.6.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase Era"
                     /protein_id="HGU4548606.1"
                     /translation="MSIDKSYCGFIAIVGRPNVGKSTLLNKLLGQKISITSRKAQTTR
                     HRIVGIHTEGAYQAIYVDTPGLHMEEKRAINRLMNKAASSSIGDVELVIFVVEGTRWT
                     PDDEMVLNKLRDGKAPVILAVNKVDNVQEKADLLPHLQFLASQMNFLDIVPISAETGL
                     NVDTIAAIVRKHLPEATHHFPEDYITDRSQRFMASEIIREKLMRFLGAELPYSVTVEI
                     ERFVSNERGGYDINGLILVEREGQKKMVIGNKGAKIKTIGIEARKDMQEMFEAPVHLE
                     LWVKVKSGWADDERALRSLGYVDDL"
     gene            complement(3328..4008)
                     /gene="rnc"
                     /locus_tag="ACHOSC_RS13465"
                     /old_locus_tag="pgaptmp_002693"
     CDS             complement(3328..4008)
                     /gene="rnc"
                     /locus_tag="ACHOSC_RS13465"
                     /old_locus_tag="pgaptmp_002693"
                     /EC_number="3.1.26.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015960048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004525 - ribonuclease III activity
                     [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease III"
                     /protein_id="HGU4548607.1"
                     /translation="MNPIVINRLQRKLGYTFNHQELLQQALTHRSASSKHNERLEFLG
                     DSILSYVIANALYHRFPRVDEGDMSRMRATLVRGNTLAELAREFELGECLRLGPGELK
                     SGGFRRESILADTVEALIGGVFLDSDIQTVEKLILNWYQTRLDEISPGDKQKDPKTRL
                     QEYLQGRHLPLPTYLVVQVRGEAHDQEFTIHCQVSGLSEPVVGTGSSRRKAEQAAAEQ
                     ALKKLELE"
     gene            complement(4281..5255)
                     /gene="lepB"
                     /locus_tag="ACHOSC_RS13470"
                     /old_locus_tag="pgaptmp_002694"
     CDS             complement(4281..5255)
                     /gene="lepB"
                     /locus_tag="ACHOSC_RS13470"
                     /old_locus_tag="pgaptmp_002694"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HGU4548608.1"
                     /translation="MANMFALILVIATLVTGILWCVDKFFFAPKRRERQAAAQAAAGD
                     SLDKATLKKVAPKPGWLETGASVFPVLAIVLIVRSFIYEPFQIPSGSMMPTLLIGDFI
                     LVEKFAYGIKDPIYQKTLIETGHPKRGDIVVFKYPEDPKLDYIKRAVGLPGDKVTYDP
                     VSKELTIQPGCSSGQACENALPVTYSNVEPSDFVQTFSRRNGGEATSGFFEVPKNETK
                     ENGIRLSERKETLGDVTHRILTVPIAQDQVGMYYQQPGQQLATWIVPPGQYFMMGDNR
                     DNSADSRYWGFVPEANLVGRATAIWMSFDKQEGEWPTGVRLSRIGGIH"
     gene            complement(5271..7070)
                     /gene="lepA"
                     /locus_tag="ACHOSC_RS13475"
                     /old_locus_tag="pgaptmp_002695"
     CDS             complement(5271..7070)
                     /gene="lepA"
                     /locus_tag="ACHOSC_RS13475"
                     /old_locus_tag="pgaptmp_002695"
                     /EC_number="3.6.5.n1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation elongation factor 4"
                     /protein_id="HGU4548609.1"
                     /translation="MKNIRNFSIIAHIDHGKSTLSDRIIQICGGLSDREMEAQVLDSM
                     DLERERGITIKAQSVTLDYKASDGETYQLNFIDTPGHVDFSYEVSRSLAACEGALLVV
                     DAGQGVEAQTLANCYTAMEMDLEVVPVLNKIDLPAADPERVAEEIEDIVGIDATDAVR
                     CSAKTGVGVQDVLERLVRDIPPPEGDPEGPLQALIIDSWFDNYLGVVSLIRIKNGTLR
                     KGDKVKVMSTGQTYNADRLGIFTPKQVDRTELKCGEVGWLVCAIKDIHGAPVGDTLTL
                     ARNPAEKALPGFKKVKPQVYAGLFPVSSDDYEAFRDALGKLSLNDASLFYEPESSSAL
                     GFGFRCGFLGLLHMEIIQERLEREYDLDLITTAPTVVYEVETTSREVIYVDSPSKLPA
                     VNNIYELREPIAECHMLLPQAYLGNVITLCVEKRGVQTNMVYHGNQVALTYEIPMAEV
                     VLDFFDRLKSTSRGYASLDYNFKRFQASDMVRVDVLINGERVDALALITHRDNSQNRG
                     RELVEKMKDLIPRQQFDIAIQAAIGTHIIARSTVKQLRKNVLAKCYGGDISRKKKLLQ
                     KQKEGKKRMKQIGNVELPQEAFLAILHVGKDNK"
     gene            complement(7268..7747)
                     /gene="rseC"
                     /locus_tag="ACHOSC_RS13480"
                     /old_locus_tag="pgaptmp_002696"
     CDS             complement(7268..7747)
                     /gene="rseC"
                     /locus_tag="ACHOSC_RS13480"
                     /old_locus_tag="pgaptmp_002696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SoxR-reducing system protein RseC"
                     /protein_id="HGU4548610.1"
                     /translation="MIKEWATVVSWQNGQALVSCDVKVSCSSCASRAGCGSRVLNKLG
                     PQTTHTIVVPCDEPLVPGQKVELGIAEGSLLSSALLVYMSPLVGLFLIASLFQLLFAS
                     DVAALCGAVLGGIGGFLIARGYSRKFAARAEWQPIILSVALPPGLVRFETSSEDASQ"
     gene            complement(7744..8700)
                     /gene="rseB"
                     /locus_tag="ACHOSC_RS13485"
                     /old_locus_tag="pgaptmp_002697"
     CDS             complement(7744..8700)
                     /gene="rseB"
                     /locus_tag="ACHOSC_RS13485"
                     /old_locus_tag="pgaptmp_002697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-E factor regulatory protein RseB"
                     /protein_id="HGU4548611.1"
                     /translation="MKQLWFAMSLVTGSLLFSANASATPASGALLQQMNLASQSLNYE
                     LSFISINKQGVESLRYRHARLDNRPLAQLLQMDGPRREVVQRGNEISYFEPGLEPFTL
                     NGDYIVDSLPSLIYTDFKRLSPYYDFISVGRTRIADRLCEVIRVVARDGTRYSYIVWM
                     DIESKLPMRVDLLDRDGETLEQFRVIAFNVNQDISSSMQTLAKANLPPLLSVPVGEKA
                     KFSWTPTWLPQGFSEVSSSRRPLPTMDNMPIESRLYSDGLFSFSVNVNRATPSSTDQM
                     LRTGRRTVSTSVRDNAEITIVGELPPQTAKRIAENIKFGAAQ"
     gene            complement(8700..9350)
                     /gene="rseA"
                     /locus_tag="ACHOSC_RS13490"
                     /old_locus_tag="pgaptmp_002698"
     CDS             complement(8700..9350)
                     /gene="rseA"
                     /locus_tag="ACHOSC_RS13490"
                     /old_locus_tag="pgaptmp_002698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016989 - sigma factor antagonist activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anti-sigma-E factor RseA"
                     /protein_id="HGU4548612.1"
                     /translation="MQKEQLSALMDGETLDSELLNELAHNPEMQKTWESYHLIRDSMR
                     GDTPEVLHFDISSRVMAAIEEEPVRQPATLIPEAQPAPHQWQKMPFWQKVRPWAAQLT
                     QMGVAACVSLAVIVGVQHYNGQSETSQQPETPVFNTLPMMGKASPVSLGVPSEATANN
                     GQQQQVQEQRRRINAMLQDYELQRRLHSEQLQFEQAQTQQAAVQVPGIQTLGTQSQ"
     gene            complement(9383..9958)
                     /gene="rpoE"
                     /locus_tag="ACHOSC_RS13495"
                     /old_locus_tag="pgaptmp_002699"
     CDS             complement(9383..9958)
                     /gene="rpoE"
                     /locus_tag="ACHOSC_RS13495"
                     /old_locus_tag="pgaptmp_002699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005070454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoE"
                     /protein_id="HGU4548613.1"
                     /translation="MSEQLTDQVLVERVQKGDQKAFNLLVVRYQHKVASLVSRYVPSG
                     DVPDVVQEAFIKAYRALDSFRGDSAFYTWLYRIAVNTAKNYLVAQGRRPPSSDVDAIE
                     AENFESGGALKEISNPENLMLSEELRQIVFRTIESLPEDLRMAITLRELDGLSYEEIA
                     AIMDCPVGTVRSRIFRAREAIDNKVQPLIRR"
     gene            complement(9955..10110)
                     /gene="rseD"
                     /locus_tag="ACHOSC_RS13500"
                     /old_locus_tag="pgaptmp_002700"
     CDS             complement(9955..10110)
                     /gene="rseD"
                     /locus_tag="ACHOSC_RS13500"
                     /old_locus_tag="pgaptmp_002700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rpoE leader peptide RseD"
                     /protein_id="HGU4548614.1"
                     /translation="MIRLQHDKQKQMRYGTLQKRDTLTLCLLKLQLMEWRFDSAWKFG
                     LGRLYLG"
     gene            10366..11988
                     /gene="nadB"
                     /locus_tag="ACHOSC_RS13505"
                     /old_locus_tag="pgaptmp_002701"
     CDS             10366..11988
                     /gene="nadB"
                     /locus_tag="ACHOSC_RS13505"
                     /old_locus_tag="pgaptmp_002701"
                     /EC_number="1.4.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008734 - L-aspartate oxidase activity
                     [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-aspartate oxidase"
                     /protein_id="HGU4548615.1"
                     /translation="MNTLPEHSCDVLIIGSGAAGLSLALRLADQHQVIVLSKGPVTEG
                     STFYAQGGIAAVFDETDSIDSHVEDTLIAGAGICDRHAVEFVASNARSCVQWLIDQGV
                     LFDTHVQPNGEESYHLTREGGHSHRRILHAADATGREVQSTLVSKAQNHPNIRVLERS
                     NAVDLIVSDKIGLPGTRRVVGAWVWNRNKETVETCHAKAVVLATGGASKVYQYTTNPD
                     ISSGDGIAMAWRAGCRVANLEFNQFHPTALYHPQARNFLLTEALRGEGAYLKRPDGTR
                     FMPDFDERGELAPRDIVARAIDHEMKRLGADCMFLDISHKPADFIRQHFPMIYEKLLG
                     LGIDLTQEPVPIVPAAHYTCGGVMVDDHGRTDVEGLYAIGEVSYTGLHGANRMASNSL
                     LECLVYGWSAAEDITRRMPDAHGVSTLPPWDESRVENPDERVVIQHNWHELRLFMWDY
                     VGIVRTTKRLERALRRITMLQQEIDEYYAHFRVSNNLLELRNLVQVAELIVRCAMMRK
                     ESRGLHFTLDYPELLTHSGPSILSPGNHYINR"
     gene            complement(11973..12710)
                     /gene="trmN"
                     /locus_tag="ACHOSC_RS13510"
                     /old_locus_tag="pgaptmp_002702"
     CDS             complement(11973..12710)
                     /gene="trmN"
                     /locus_tag="ACHOSC_RS13510"
                     /old_locus_tag="pgaptmp_002702"
                     /EC_number="2.1.1.223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417070.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA(1)(Val)
                     (adenine(37)-N(6))-methyltransferase TrmN"
                     /protein_id="HGU4548616.1"
                     /translation="MSQSTSVLRRNGFTFKQFFVAHDRCAMKVGTDGILLGAWAPVAG
                     VKRCLDIGAGSGLLALMLAQRTSDSVIIDAVELESEAAAQAQENINQSPWAERINVHT
                     ADIQQWITQQTVRFDLIISNPPYYQQGVECATPQREQARYTTTLDHPSLLTCAAECIT
                     EEGFFCVVLPEQIGNGFTELALSMGWHLRLRTDVAENEARLPHRVLLAFSPQAGECFS
                     DRLVIRGPDQNYSEAYTALTQAFYLFM"
     gene            12842..14176
                     /gene="srmB"
                     /locus_tag="ACHOSC_RS13515"
                     /old_locus_tag="pgaptmp_002703"
     CDS             12842..14176
                     /gene="srmB"
                     /locus_tag="ACHOSC_RS13515"
                     /old_locus_tag="pgaptmp_002703"
                     /EC_number="3.6.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003724 - RNA helicase activity [Evidence
                     IEA];
                     GO_process: GO:0000027 - ribosomal large subunit assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RNA helicase SrmB"
                     /protein_id="HGU4548617.1"
                     /translation="MTVTTFSELELDESLLEALQDKGFTRPTAIQAAAIPPALDGRDV
                     LGSAPTGTGKTAAYLLPALQHLLDFPRKKSGPPRILILTPTRELAMQVADHARELAKH
                     THLDIATITGGVAYMNHAEVFSENQDIVVATTGRLLQYIKEENFDCRAVETLILDEAD
                     RMLDMGFAQDIEHIAGETRWRKQTLLFSATLEGDAIQDFAERLLEDPVEVSANPSTRE
                     RKKIHQWYYRADDLEHKTALLVHLLKQPEATRSIVFVRKRERVHELANWLREAGINNC
                     YLEGEMVQGKRNEAIKRLTEGRVNVLVATDVAARGIDIPDVSHVFNFDMPRSGDTYLH
                     RIGRTARAGRKGTAISLVEAHDHLLLGKVGRYIEEPIKARVIDELRPKTRAPSEKQTG
                     KPSKKVLAKRAEKKKAKEKEKPRVKKRHRDTKNIGKRRKPSGTGVPPQTTEE"
BASE COUNT         3419 a         3817 c         3556 g         3448 t
ORIGIN      
        1 tatagttatt aaaggcgcgt ccgattggtt caccggacgc accttaagta cgtttccttg
       61 tgttataaga acagaaggat cagctgtaaa acagcaatga tgattttgat gacccgttta
      121 atcaggtatc ggcaatcagt cattcgtttt tccttaaaca aggaatgcag ccgttcagat
      181 tagcccttac atctccccaa aactgaacgt gcgagttatt gagggtgcat gctgcactcc
      241 acaccagagc tttgacgaca ccactcgttt caatggggga attctgtggc atggtgtaaa
      301 gcacagcaaa atcttcaata acgaagccaa ttttaatgta cttacgaatt ggcggtcacg
      361 ttaatctttc catcaatatt gctttcttcg taaaggctcg agtttttatg ctaaagattg
      421 caagttgctt gtaaaagata agtacactga tccataatcg ctgttgttga gggtgcatgc
      481 tgcacaaaat taaagttaaa aagtaaaacc cccgttcctt accagttcgg gggttttact
      541 ttttaaagag aacggtatta ttttaacttt caataattac cgtggcacaa gcatagtggc
      601 gttcatctgc cagcgttaca tgcatattta caacgccgag cttttccgcc agttttaatg
      661 cctcacccca tagccgtagc cgtggtttgc cgagctcatc attgaatact tcaaattgat
      721 taaacgccag accattgcgg atcccggtgc caaacgcttt tgctgcggct tctttcacag
      781 caaaacgctt cgccagaaaa cgcaccggct ggtggtgcgt tttccagatt gcccattcgt
      841 tatcgcttaa tacgcggcgt gccaggcgct caccggatcg ggcgatcact gcttctatgc
      901 gagcgatctc tacgatatcc gtgcctaaac ctaatattgc cattagccac gcgcttccag
      961 catcagacgc ttcatttctg ccaccgcatc tttcagtccg gtcatcactg cacgaccaat
     1021 aatggcatga ccgatattca gttcatgcat ctcagggatg gcggcaatag ctttcacgtt
     1081 gtgataggtc agaccgtgac cagcgttaac tttcagaccg agactagcgg caaaggtcgc
     1141 ggctttggcg atacgcgcca gctcttgcgc ctgttcggcg tcagttttgg catcagcata
     1201 gcaaccggtg tggatctcga taaacggtgc gccaacctct gccgcagcct tgatctgctc
     1261 ttcatcggcg tcgataaaga gagaaacctg gatcccggca tctgccagac gtttgcaggc
     1321 atcgcgcatt ttgtcacgct gccctgcgac gtccaggccg ccttcggttg ttacttcctg
     1381 acgcttttcc ggtaccagac agcaaaaatg tggcttcgtc tcaacggcga tcgccagcat
     1441 ctcttcggtc accgccatct ccagattcat gcgggtatcc agcgtctgac gcaggatgcg
     1501 cacatcacgg tcagtaatgt ggcggcgatc ttcacgtaaa tgcacggtaa tgccgtccgc
     1561 tcccgcctgc tcagcaataa acgccgcctg caccggatcc gggtaagcag taccgcgtgc
     1621 gttacgcagc gttgcaatat ggtcaatgtt gacgcctaac agtaattcag ccatgacaat
     1681 cctcatcatt cataatgtgt tttcaccgtt cgcttaggca taaactgccg gaacagttcc
     1741 ctgctcttta atggtttacc gccaagatac ggcttaagcg ccatgcgggt aaagcgtttc
     1801 gcggcgcgta gtgtgtctgc gtcaggaaat tcccgcgcgt ttaacgcttt taactgcctt
     1861 ccggtgaacg ttttattgtc gataacgacg cttgcgataa accctttttc ttcgcgataa
     1921 cgatacgtca tggtgtcatc taccggctcg ccgctacccg cacaatgggt aaaattgacg
     1981 ccatagccca gatgcccgag cagtgccagt tcaaagcggc gcagcgcggg ttctggcgta
     2041 ccagtggcac ctgcaagaga ctgaatgcag tgcaagtaat cgaaaaagag ttcagagaag
     2101 cgcgtctcgt attccagtac gcgggagaga agttcgttga tgtacagacc gctgtaaagc
     2161 gtgataccgc ttaatggcag cgccagcgag acagcttcag cactgcgcag cgttttgact
     2221 tcgccacgcc cgccgaaacg tagcaagaga ggggtgaaag gctgtaatgc acctttcaga
     2281 gtagagcgtt tagagcgtgc gcctttggca accagacgca cgcgacccga ttcctccgtg
     2341 aagacgtcca gcatcaggct ggtttcgctc cacgggcgac tatgcaggac aaatgcgcgc
     2401 tgccagcctt ccatcggagt gactcttaaa gatcgtcaac gtaaccgaga ctgcgcagtg
     2461 cgcgttcgtc gtcggcccaa ccggatttca cttttaccca cagttcaagg tggacaggcg
     2521 cttcgaacat ttcctgcatg tctttacgcg cttcaatccc gatggttttg atcttggccc
     2581 ctttgttgcc aataaccatc ttcttctgcc cttcacgctc aacgagaatc aaaccattga
     2641 tgtcataacc accgcgttcg ttagagacga aacgttcgat ctccacggtc acggagtacg
     2701 gcagttcagc gccgaggaaa cgcatcagtt tttcgcggat gatttcagac gccataaaac
     2761 gctgtgagcg atcggtgatg taatcttccg ggaagtgatg ggtcgcttca gggagatgct
     2821 tacgcacgat tgctgcgata gtgtcgacat tcagcccggt ttcagcagag atcggcacga
     2881 tatcgaggaa gttcatctga cttgccagga actgtaagtg cggcagcaga tcggcttttt
     2941 cctgcacgtt atccactttg ttcaccgcga ggatcaccgg cgctttgcca tcgcgcagtt
     3001 tgttgagcac catttcgtcg tccggcgtcc agcgggtgcc ttcaacgaca aaaatcacca
     3061 gctcaacgtc gccaatagag ctgctcgccg ctttgttcat caggcggtta atagcgcgtt
     3121 tttcttccat atgcaggcca ggggtatcaa cgtagatcgc ctgatacgcg ccttcagtat
     3181 ggatccccac aatgcggtga cgagttgttt gcgctttgcg ggaggtgatg gagatcttct
     3241 gccccagcag tttgttcaac aatgtggatt tgccaacgtt cggacgtccg acgatggcaa
     3301 taaatccgca gtaactttta tcgatgctca ttccagctcc agttttttca acgcctgttc
     3361 ggcggcagcc tgctcagcct tacgacggct tgaacctgtg ccaaccaccg gttcactcag
     3421 gccgctgacc tggcagtgga tagtaaattc ctgatcgtgc gcttcgccac gtacctggac
     3481 taccagataa gtcggcagcg gcagatggcg accctgcaaa tattcttgca agcgcgtttt
     3541 cggatctttt tgtttatcgc ctgggctaat ttcgtccaaa cgagtttgat accagttgag
     3601 gattaatttc tcgacggttt gaatatcact gtcgaggaat acaccaccaa ttaatgcttc
     3661 gacggtgtcg gcgagaattg actcacgacg aaatccaccg cttttaagtt cacctggccc
     3721 taaacgtaag cactcgccta actcaaattc gcgcgccagt tccgccagcg tattgccacg
     3781 gaccagcgtg gcgcgcatcc ggctcatatc gccttcatcc acacgaggga aacggtgata
     3841 aagcgcattg gcgataacgt agctcagaat agagtcgcct aaaaattcta aacgctcgtt
     3901 atgtttgctg ctggcactac gatgagttaa tgcctgctgc aacagttcct gatgattaaa
     3961 agtgtagccc agcttccgtt gaagccgatt aattacgatg gggttcatgc gataccaata
     4021 aataaatgcg tcaacaattc agcacacgaa acagacctga tatacatggc tctgctaact
     4081 gcttcgctgc agtttcagtt taccggtata tggggaccaa cgctgtttcg tgtgccgtgg
     4141 caacctggag gtgccaacct taaacttcgg gggaatattc tatacacaac gacgggggat
     4201 gtcgttagcc acgggagatt tatctcataa ataattcacg ttgtcgccat aacggcgaca
     4261 acgtgaacga agatgggcta ttaatggatg ccgccaatgc gacttaagcg cacacctgtc
     4321 ggccattcgc cttcttgctt atcgaaactc atccagatag ccgttgcccg accgaccaga
     4381 ttcgcttcag gcacaaaacc ccagtaacgg ctgtccgcgc tgttgtcgcg gttgtcgccc
     4441 atcatgaagt attgtcccgg cggaacaatc caggttgcca gttgttgccc tggctgctgg
     4501 taatacatcc ccacctggtc ctgcgcaatc ggcactgtca gaattcggtg cgtcacatca
     4561 cccagtgtct ctttacgctc ggaaagacga attccatttt ctttggtttc gtttttcggc
     4621 acttcaaaga atccgctggt cgcttcccca ccattacggc gtgagaaggt ctgaacgaaa
     4681 tcgctcggtt ccacgttgga gtaggtgacc ggtagcgcgt tttcacacgc ctggccggaa
     4741 ctgcatcccg gttgaatcgt cagctctttt gagaccggat cgtaagtgac tttatcgccc
     4801 ggtaaaccca ccgcgcgctt gatgtaatca agctttggat cttccggata tttaaagacc
     4861 acgatatcgc cgcgtttcgg atgaccggtt tcgatcagcg ttttctggta gataggatct
     4921 ttaatgccat aagcaaactt ctctaccaga ataaaatcac cgattaacag agtcggcatc
     4981 atcgaacctg acgggatctg gaacggttca taaataaacg aacgtacaat caatacgata
     5041 gccagcaccg gaaaaacaga agcaccggtt tccagccagc caggcttcgg cgcaaccttt
     5101 ttcagggttg ctttatccag tgagtcaccg gcagccgcct gcgccgctgc ctgacgttcc
     5161 cgccgtttag gtgcgaaaaa gaatttatcc acgcaccata aaatgcccgt caccagtgtg
     5221 gcaatcacca gaatcagggc aaacatattc gccatgccaa ctcctaaggg ttatttgttg
     5281 tctttgccaa cgtgcagaat ggcgaggaac gcttcctgcg gcagctcgac gttaccgatc
     5341 tgcttcatgc gtttcttacc ttctttctgc ttctgcagca gctttttctt acggctgata
     5401 tcgccgccat aacatttagc cagtacgttt ttacgtagct gtttcacggt ggatcgcgca
     5461 ataatgtgcg taccaatcgc tgcctgaatg gcgatatcaa actgctggcg tgggatcaga
     5521 tctttcatct tctccaccaa ctcgcgaccg cggttttgcg aattatcacg gtgggtgatc
     5581 agcgccaacg catcaacacg ttcaccgttg attaatacgt ctacacgtac catgtcggac
     5641 gcctggaagc gtttgaagtt gtaatccaga gacgcataac cacgcgaggt agatttcagg
     5701 cgatcgaaga agtcgagcac cacttccgcc atcgggatct cgtacgtcag cgccacctga
     5761 ttaccgtggt aaaccatatt ggtctgcacg ccgcgttttt ctacgcacag cgtaataacg
     5821 ttgccgagat atgcctgcgg cagcagcatg tgacattcgg caatcggctc gcgcagttcg
     5881 tagatgttat ttaccgctgg cagcttggat ggactgtcga cgtaaataac ttctcttgac
     5941 gtggtttcaa cttcatacac tacggtcggc gcagtggtga tcagatccag atcgtattca
     6001 cgttccagac gttcctggat gatctccatg tgcagcaggc cgaggaagcc gcagcggaaa
     6061 ccaaagccca gcgcgctgga gctttccggc tcatagaaca gtgaagcatc gttcaggctg
     6121 agtttaccca gcgcgtcacg gaaggcttca tagtcgtcgg aacttaccgg gaacagaccg
     6181 gcgtatacct gcggtttgac tttcttaaag ccaggcagcg ccttttctgc cggattacgc
     6241 gccagcgtta aggtatcgcc gactggagcg ccgtggatat ctttaatcgc acataccagc
     6301 cagcctactt cgccacattt cagttcagtg cggtcaacct gtttcggcgt gaagatgccc
     6361 agacggtcgg cgttataggt ctgcccggta ctcatgactt tcactttgtc gcccttacgc
     6421 agggtgccgt ttttaatacg gataagtgaa acaacgccca ggtagttgtc gaaccatgag
     6481 tcgataatta gtgcctgcaa cgggccttcc ggatcgcctt ccggcggcgg aatgtcgcgc
     6541 accagacgtt cgagaacgtc ctgcacgcca acgccggttt tcgctgaaca gcgcaccgcg
     6601 tcggtggcgt cgatgccgac gatatcttca atttcttccg ccacgcgttc aggatcggct
     6661 gccggcaggt caatcttgtt cagtaccggc accacttcga gatccatttc catggcggtg
     6721 tagcagtttg ccagggtttg cgcttctacg ccctgcccgg cgtcgaccac cagcaatgca
     6781 ccttcacagg cagccagcga acgggaaact tcataggaga agtcaacgtg gcccggggtg
     6841 tcgataaagt tcagctgata ggtttcgccg tcagacgctt tgtagtccag cgtcacgctt
     6901 tgcgctttga tggttatgcc acgctcacgc tcaagatcca tggaatcgag aacctgcgcc
     6961 tccatttcac ggtcagacag gccaccgcag atctggataa tacggtcaga cagcgtcgat
     7021 ttaccgtggt caatgtgagc tatgatcgaa aagttacgta tattcttcat aaagtatgat
     7081 tattgtgcct taatgcccgg ttaaccaggc ttttagaagt cgctgttctg agcttaacgt
     7141 ctgtattaat agaaacgccg cattctacac tacaacattg aggcgaggaa atgttcatac
     7201 cgtatggatt gtggtatctg gaaacgtcct cgcatttgtt atgcaaaatg caacaaagcc
     7261 agtgaaatca ctggctcgcg tcttccgaag atgtttcaaa tcgcacaagg ccaggcggca
     7321 atgccacgct taagatgatc ggttgccatt ctgcccgggc agcaaactta cgcgagtagc
     7381 cgcgcgcaat caggaacccg ccaataccgc cgaggaccgc accacataat gctgcgacgt
     7441 cagaagcaaa gagtagctga aatagcgaag cgatgaggaa taatcccacc agcggcgaca
     7501 tataaaccag taatgcggag ctaagcaggc tgccttcggc gatccccaat tccacttttt
     7561 gccccggcac taacggctca tcacagggta cgacaatggt atgcgtggtt tgcgggccaa
     7621 gtttatttaa cacgcggcta ccgcaaccgg cgcgtgaagc gcagctgctg catgaaactt
     7681 taacatcaca actgaccagc gcctgcccgt tttgccagga gacgacggta gcccactctt
     7741 tgatcattgc gctgccccga acttaatatt ctcggcaatg cgtttcgccg tttgcggcgg
     7801 cagttcaccg acaatggtga tttcggcgtt atcacgtacg cttgtactga cggttctgcg
     7861 tccggtgcgc aacatctgat cggtgctcga tggcgtagcg cggttaacgt ttaccgagaa
     7921 gctgaataat ccgtcggaat agagacgtga ttcgataggc atgttgtcca tcgtcggtag
     7981 cggacgtcga ctactggaaa cttcgctaaa accctgtggc aaccaggttg gcgtccagct
     8041 gaatttagct ttttcaccta caggaacaga aagcaacggc ggcaaatttg ccttcgccag
     8101 cgtctgcata ctgctactga tatcctgatt gacgttaaaa gcaatcacgc gaaattgttc
     8161 cagcgtttca ccatcgcgat caaggagatc aaccctcatc ggtaatttcg attcgatgtc
     8221 catccacacg atgtagctgt agcgtgtacc atctcgggca accacgcgaa tgacttcgca
     8281 aagacgatca gcaatacgcg tgcgcccgac ggagataaag tcgtagtaag gagaaaggcg
     8341 tttgaaatcg gtatagatga gcgatggcag agaatcaaca atgtaatcgc cattaagcgt
     8401 gaacggttca agtcccggtt caaaatagct gatttcattg ccacgctgta ccacttcccg
     8461 gcgcgggcca tccatttgca acaattgtgc aagaggacgg ttatcgaggc gtgcatgtcg
     8521 ataacgcaga gactcaacac cctgtttatt gatgctgatg aatgacagct cgtaattcag
     8581 tgactgactg gccaggttca tctgctgtaa taacgccccg gacgcgggag tggccgaggc
     8641 gttagcagag aataacaggc tacctgtcac taatgacatg gcaaaccaaa gttgcttcat
     8701 tactgcgatt gcgttcctaa agtttgaatt cctggcacct gtacagcggc ttgctgggtt
     8761 tgcgcctgct caaactgaag ctgttcagag tggagtcggc gttgcagttc gtaatcctgc
     8821 aacattgcat taatgcgacg acgctgctcc tgtacctgct gctgttgacc attgtttgcg
     8881 gtcgcttcag aaggtactcc caggcttacc gggctggctt tacccatcat cggcagtgta
     8941 ttaaataccg gcgtttcggg ctgctgggac gtttcagatt gtccattata gtgctggacg
     9001 ccaacgataa ctgcaagcga tacgcatgct gctacgccca tttgggtaag ctgtgccgcc
     9061 cacggacgta ctttctgcca gaatggcatt ttctgccatt gatgcggcgc aggctgggct
     9121 tccgggatca atgtcgccgg ttgacgtact ggctcttctt caatggcggc catcacgcgt
     9181 gaagagatat cgaaatggag cacctcggga gtatcacccc gcattgagtc acggattaag
     9241 tgatagcttt cccaggtttt ctgcatttct gggttatgag ccagttcgtt aagcagctca
     9301 ctatccagcg tttcgccatc cattaaagcg gaaagttgtt ctttctgcat gcctaatacc
     9361 cttatccagt atcccgctat cgtcaacgcc tgataagcgg ttgaactttg ttatcaatag
     9421 cttccctcgc tcggaagata cgtgaacgca ccgtacctac cggacaatcc atgatagcgg
     9481 ctatctcttc atagctcagg ccatccagct cccgcaaggt tattgccatg cgcaaatctt
     9541 ccgggaggga ctcaatagtt cggaaaacta tctgtctcag ttcttctgac aacattaagt
     9601 tctcagggtt cgaaatttct ttcaacgcgc cgccactttc gaagttttca gcttcaatgg
     9661 catccacatc actggaaggt ggacgacgcc cctgagcaac caggtaattt ttcgctgtat
     9721 ttacagcaat ccgatacagc catgtataaa aagcgctatc tccccggaac gaatccagcg
     9781 cacgataggc tttaataaaa gcttcttgta ccacatcggg aacatcaccc gacggcacat
     9841 agcgggaaac cagactcgcc actttatgct gatagcgcac taccagtagg ttaaaggctt
     9901 tctgatctcc cttctggacc cgttcaacca ggacctggtc cgttaactgc tcgctcatcc
     9961 gaggtaaagt ctccccaaac caaatttcca cgcgctatcg aaacgccact ccattagctg
    10021 caatttgagc aagcaaaggg ttagagtgtc tcgtttttgt aaagttccgt aacgcatctg
    10081 tttttgtttg tcatgctgta gacggatcat tatctatcat tataagtcta cagaatctga
    10141 acatcgcatt atctgtgtag aaatgcccat ttaactgcct gaagagtaac ccaacggcct
    10201 ttttatttca ccacctaatc ctccaccagc cagtaacttc tctttttctc gccgccctgc
    10261 gtcagcgtgt ttagccactg taacaaatat taaaatagca ggtgtttatt cgcacaacat
    10321 gatgctatgc tgaccaaacg gtgtttagta aattaaacaa agaaaatgaa tactctccca
    10381 gaacattcat gtgacgtgtt gattatcggt agcggcgcag ccggactttc actggcgcta
    10441 cgcctggctg accagcatca ggtcatcgtt ctaagtaaag gcccggtaac ggaaggttca
    10501 acattttatg cccagggcgg tattgccgcc gtgtttgatg aaactgacag cattgactcg
    10561 catgtggaag acacattgat tgccggggct ggtatttgcg atcgccatgc agttgaattt
    10621 gtcgccagca atgcacgatc ctgtgtgcaa tggttaattg accaaggggt tttgtttgat
    10681 acccacgttc aaccgaatgg cgaagaaagc taccatctga cccgtgaagg tggacatagt
    10741 caccgtcgta ttcttcatgc cgccgacgcc accggtagag aagtacaatc cacgctggtg
    10801 agcaaggcgc agaaccatcc gaatattcgt gtgctggagc gcagcaacgc agttgatctg
    10861 attgtttctg acaaaattgg cctcccgggc acgcgacggg ttgttggcgc gtgggtatgg
    10921 aaccgaaata aagaaacggt ggaaacctgc cacgcaaaag cagtggtact ggcaaccggc
    10981 ggtgcgtcga aggtttatca gtacaccacc aatccggata tttcttctgg cgatggcatt
    11041 gctatggcgt ggcgcgcagg ctgtcgggtt gccaatctcg aatttaatca gttccaccct
    11101 accgcactgt atcacccaca ggcacgcaat ttcctgttaa cggaagcact gcgcggcgaa
    11161 ggcgcttatc tcaagcgccc ggatggcacg cgttttatgc ccgattttga tgagcgcggc
    11221 gaactggccc cgcgcgatat tgtcgcccgc gccattgacc atgaaatgaa acgcctcggc
    11281 gcagattgta tgttcctcga catcagccat aagcccgccg attttattcg ccagcatttc
    11341 ccgatgattt atgaaaaatt gctcgggctg gggattgatc tcacacaaga accggtaccg
    11401 attgtgcctg ctgcacatta tacctgcggt ggtgtaatgg ttgatgatca tgggcgtacg
    11461 gacgtcgagg gcttgtatgc cattggcgag gtgagttata ccggcttaca cggcgctaac
    11521 cgcatggcct cgaattcatt gctggagtgt ctggtctatg gctggtcggc ggcagaagat
    11581 atcaccagac gtatgcctga tgcccacggc gtcagtacgt taccgccgtg ggatgaaagc
    11641 cgcgttgaga accctgacga acgggtagta attcagcata actggcacga gctacggctg
    11701 tttatgtggg attacgttgg cattgtgcgc acaacgaagc gcctggaacg cgccctgcgg
    11761 cggataacca tgctccaaca agaaatagac gaatattacg cccatttccg cgtctcaaat
    11821 aatttgctgg agctgcgtaa tctggtacag gttgccgagt tgattgttcg ctgcgcgatg
    11881 atgcgcaaag aaagccgggg gctgcatttt acgctggatt acccggaact gctcacgcat
    11941 tccggtccat cgatcctttc ccccggcaat cattacataa acagataaaa agcctgggtc
    12001 agcgccgtat acgcttcgga atagttctgg tctggcccac gaatgactaa gcgatcgcta
    12061 aagcattctc ccgcctgcgg ggagaatgcc agcagcaccc gatgcggcag tcgcgcttcg
    12121 ttttccgcca catccgtccg caaacgtaaa tgccagccca tgcttaatgc cagttcagta
    12181 aaaccattac caatctgctc tggcagcacc acgcagaaaa atccctcttc ggtaatgcac
    12241 tccgccgcac aggtcagcag cgatgggtga tcaagcgtag tggtatagcg agcctgttcc
    12301 cgttgaggtg tcgcgcactc tactccctgc tgatagtaag gtgggttact gatgattaaa
    12361 tcgaagcgta ctgtctgctg tgtgatccac tgctgaatat ccgccgtatg gacgttaatc
    12421 cgctctgccc acggggactg gttgatattt tcctgcgcct gcgcggcagc ttcactttcc
    12481 agctcaacgg catcaattat tacgctgtca ctggttcgct gcgccagcat cagtgccagc
    12541 aacccgctac ccgcgccgat atcaaggcaa cgtttcaccc cagccaccgg tgcccatgcg
    12601 cccagcaaaa tgccatccgt tcccactttc atcgcacagc gatcgtgagc aacaaaaaac
    12661 tgtttaaaag taaatccatt acgacgaagc acggatgtag actgtgacat gaaaataaaa
    12721 ccttgcagga aaaacggcga tagcaccggg tgagaacaat acccgagaag cgatatccat
    12781 acaaacagat gaagattgca gccgtaacgt ctataatcag cgccccacac agaggtagaa
    12841 catgactgta acgacttttt ccgaacttga actcgacgaa agcctgctgg aagccctcca
    12901 ggataaaggt ttcactcgcc cgaccgccat tcaggctgcc gccattccgc ctgcgctcga
    12961 tggccgtgat gtactcggtt ctgcgccgac aggcaccggt aaaacggcgg cgtatctgct
    13021 gccagcgttg cagcacctgc tcgatttccc gcgtaagaaa tccggtccgc cgcgtatttt
    13081 gatcctcacc ccaactcgcg agctggcgat gcaggtggcc gatcatgccc gcgaactggc
    13141 gaaacatacg catctggata tcgccaccat caccggcggc gtagcctata tgaaccacgc
    13201 ggaagtgttc agcgaaaatc aggacatcgt ggtcgccacg accggacgtc tgctgcaata
    13261 cataaaagaa gagaacttcg attgccgcgc ggttgaaacg ctgatcctcg acgaagcaga
    13321 ccgtatgctg gatatgggct tcgctcagga tatcgaacat attgctggcg aaacgcgctg
    13381 gcgtaaacag accctgctct tttcggcaac gctggaaggc gatgcgattc aggactttgc
    13441 cgagcgtctg ctggaagatc cggtggaggt ttctgccaat ccgtccaccc gtgagcgcaa
    13501 aaaaattcat cagtggtatt accgcgccga tgacctggaa cataaaaccg cattgctggt
    13561 gcatctgtta aaacagccgg aagcgacccg ctcaattgtg tttgtgcgta agcgtgaacg
    13621 tgtgcatgag ctggcaaact ggctgcgcga agcgggcatc aacaactgct atctcgaagg
    13681 tgagatggta caaggcaagc gtaacgaagc gatcaagcgt ttgaccgaag gtcgcgtaaa
    13741 cgtactggtt gcaaccgatg ttgccgcgcg cggtatcgac attcctgacg tcagccacgt
    13801 ctttaacttc gatatgccgc gcagtggcga tacttatttg caccgtatcg gacgtaccgc
    13861 gcgcgccggt cgtaaaggca ccgcaatttc gctggtggaa gcccatgacc atttgctgct
    13921 gggcaaggtt ggccgttaca ttgaagagcc gattaaagct cgcgttattg atgagttacg
    13981 cccgaaaacg cgtgcgccaa gcgagaagca gaccggcaag ccatcgaaga aagtactggc
    14041 aaaacgcgcc gagaagaaaa aagctaaaga gaaagagaaa ccgcgtgtga aaaaacgcca
    14101 tcgcgacacc aaaaatattg gtaagcgccg taaaccaagc ggaacgggcg tgccaccgca
    14161 aacgacagaa gagtaatctc aatgccgggt ttaagcctgg tattaaaagt gctggcaaac
    14221 gcaaaactgc ctgatgcgct
//
LOCUS       DAZKHF020000113        14237 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2201S31241
            SAMN43267800-rid25106413.denovo.114, whole genome shotgun sequence.
ACCESSION   DAZKHF020000113 DAZKHF020000000
VERSION     DAZKHF020000113.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267800
            Sequence Read Archive: SRR30307263
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 14237)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14237)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 14237)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350581.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350581.2
            Genome Coverage       :: 151x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:21:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,652
            CDSs (total)                      :: 5,557
            Genes (coding)                    :: 5,320
            CDSs (with protein)               :: 5,320
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 237
            CDSs (without protein)            :: 237
            Pseudo Genes (ambiguous residues) :: 0 of 237
            Pseudo Genes (frameshifted)       :: 102 of 237
            Pseudo Genes (incomplete)         :: 130 of 237
            Pseudo Genes (internal stop)      :: 52 of 237
            Pseudo Genes (multiple problems)  :: 41 of 237
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14237
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267800-rid25106413.denovo.114"
                     /serotype="O 26:H11"
                     /isolate="2201S31241"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-01"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            127..1185
                     /gene="citC"
                     /locus_tag="ACHOSC_RS13520"
                     /old_locus_tag="pgaptmp_002704"
     CDS             127..1185
                     /gene="citC"
                     /locus_tag="ACHOSC_RS13520"
                     /old_locus_tag="pgaptmp_002704"
                     /EC_number="6.2.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415151.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008771 - [citrate (pro-3S)-lyase] ligase
                     activity [Evidence IEA];
                     GO_process: GO:0006101 - citrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="[citrate (pro-3S)-lyase] ligase"
                     /protein_id="HGU4548618.1"
                     /translation="MFGNDIFTRVKRSENKKMAEIAQFLHENDLSVDTTVEVFITVTR
                     DEKLIACGGIAGNIIKCVAISESVRGEGLALTLATELINLAYERHSTHLFIYTKTEYE
                     ALFRQCGFSTLTSVPGVMVLMENSATRLKRYAESLKKFRHPGNKIGCIVMNANPFTNG
                     HRYLIQQAAAQCDWLHLFLVKEDSSRFPYEDRLDLVLKGTADIPRLTVHRGSEYIISR
                     ATFPCYFIKEQSVINHCYTEIDLKIFRQYLAPALGVTHRFVGTEPFCRVTAQYNQDMR
                     YWLETPTISAPPIELVEIERLRYQEMPISASRVRQLLAKNDLTAIAPLVPAVTLHYLQ
                     NLLEHSRQDAAARQKTPA"
     gene            1200..1496
                     /gene="citD"
                     /locus_tag="ACHOSC_RS13525"
                     /old_locus_tag="pgaptmp_002705"
     CDS             1200..1496
                     /gene="citD"
                     /locus_tag="ACHOSC_RS13525"
                     /old_locus_tag="pgaptmp_002705"
                     /EC_number="4.1.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_component: GO:0009346 - ATP-independent citrate lyase
                     complex [Evidence IEA];
                     GO_function: GO:0008815 - citrate (pro-3S)-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0006113 - fermentation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="citrate lyase acyl carrier protein"
                     /protein_id="HGU4548619.1"
                     /translation="MKINQPAVAGTLESGDVMIRIAPLDTQDIDLQINSSVEKQFGDA
                     IRTTILDVLARYNVRGVQLNVDDKGALDCILRARLEALLARASGIPALPWEDCQ"
     gene            1493..2401
                     /gene="citE"
                     /locus_tag="ACHOSC_RS13530"
                     /old_locus_tag="pgaptmp_002706"
     CDS             1493..2401
                     /gene="citE"
                     /locus_tag="ACHOSC_RS13530"
                     /old_locus_tag="pgaptmp_002706"
                     /EC_number="4.1.3.34"
                     /EC_number="4.1.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415149.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_component: GO:0009346 - ATP-independent citrate lyase
                     complex [Evidence IEA];
                     GO_function: GO:0008815 - citrate (pro-3S)-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0006084 - acetyl-CoA metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006113 - fermentation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="citrate (pro-3S)-lyase subunit beta"
                     /protein_id="HGU4548620.1"
                     /translation="MISASLQQRKTRTRRSMLFVPGANAAMVSNSFIYPADALMFDLE
                     DSVALREKDTARRMVYHALQHPLYRDIETIVRVNALDSEWGVNDLEAVVRGGADVVRL
                     PKTDTAQDVLDIEKEILRIEKACGREPGSTGLLAAIESPLGITRAVEIAHASERLIGI
                     ALGAEDYVRNLRTERSPEGTELLFARCSILQAARSAGIQAFDTVYSDANNEAGFLQEA
                     AHIKQLGFDGKSLINPRQIDLLHNLYAPTQKEVDHARRVVEAAEAAAREGLGVVSLNG
                     KMVDGPVIDRARLVLSRAELSGIREE"
     gene            2412..3944
                     /gene="citF"
                     /locus_tag="ACHOSC_RS13535"
                     /old_locus_tag="pgaptmp_002707"
     CDS             2412..3944
                     /gene="citF"
                     /locus_tag="ACHOSC_RS13535"
                     /old_locus_tag="pgaptmp_002707"
                     /EC_number="2.8.3.10"
                     /EC_number="4.1.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009346 - ATP-independent citrate lyase
                     complex [Evidence IEA];
                     GO_function: GO:0008814 - citrate CoA-transferase activity
                     [Evidence IEA];
                     GO_function: GO:0008815 - citrate (pro-3S)-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0006113 - fermentation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="citrate lyase subunit alpha"
                     /protein_id="HGU4548621.1"
                     /translation="MTQKIEQSQRQERVAAWNRRAECDLAAFQNSPKQTYQAEKARDR
                     KLCANLEEAIRRSGLQDGMTVSFHHAFRGGDLTVNMVMDVIAKMGFKNLTLASSSLSD
                     CHAPLVEHIRQGVVTRIYTSGLRGPLAEEISRGLLAEPVQIHSHGGRVHLVQSGELNI
                     DVAFLGVPSCDEFGNANGYTGKACCGSLGYAMVDADNAKQVVMLTEELLPYPHNPASI
                     EQDQVDLIVKVDRVGDAAKIGAGATRMTTNPRELLIARSAADVIVNSGYFKEGFSMQT
                     GTGGASLAVTRFLEDKMRSRDIRADFALGGITATMVDLHEKGLIRKLLDVQSFDSHAA
                     QSLARNPNHIEISANQYANWGSKGASVDRLDVVVLSALEIDTQFNVNVLTGSDGVLRG
                     ASGGHCDTAIASALSIIVAPLVRGRIPTLVDNVLTCITPGSSVDILVTDHGIAVNPAR
                     PELAERLQEAGIKVVSIEWLRERARLLTGEPQPIEFTDRVVAVVRYRDGSVIDVVHQV
                     KE"
     gene            3948..4499
                     /gene="citX"
                     /locus_tag="ACHOSC_RS13540"
                     /old_locus_tag="pgaptmp_002708"
     CDS             3948..4499
                     /gene="citX"
                     /locus_tag="ACHOSC_RS13540"
                     /old_locus_tag="pgaptmp_002708"
                     /EC_number="2.7.7.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050519 - holo-citrate lyase synthase
                     activity [Evidence IEA];
                     GO_process: GO:0051191 - prosthetic group biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="citrate lyase holo-[acyl-carrier protein]
                     synthase"
                     /protein_id="HGU4548622.1"
                     /translation="MHLLPELASHHAVSIPELLVSRDERQARQHVWLKRHPVPLVSFT
                     VVAPGPIKDSEVTRRIFNHGVTALRALAAKQGWQIQEQAALVSASGPEGMLSIAAPAR
                     DLKLATIELEHSHPLGRLWDIDVLTPEGEILSRRDYSLPPRRCLLCEQSAAVCARGKT
                     HQLTDLLNRMEALLNDVDACNVN"
     gene            4474..5352
                     /gene="citG"
                     /locus_tag="ACHOSC_RS13545"
                     /old_locus_tag="pgaptmp_002709"
     CDS             4474..5352
                     /gene="citG"
                     /locus_tag="ACHOSC_RS13545"
                     /old_locus_tag="pgaptmp_002709"
                     /EC_number="2.4.2.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="triphosphoribosyl-dephospho-CoA synthase CitG"
                     /protein_id="HGU4548623.1"
                     /translation="MSMPATSTKTTKLATSLIDEYALLGWRAMLTEVNLSPKPGLVDR
                     INCGAHKDMALEDFHRSALAIQGWLPRFIEFGACSAEMAPEAVLHGLRPIGMACEGDM
                     FRATAGVNTHKGSIFSLGLLCAAIGRLLQLNQPVTPTTVCSTAASFCRGLTDRELRTN
                     NSQLTAGQRLYQQLGLTGARGEAEAGYPLVINHALPHYLTLLDQGLDPELALLDTLLL
                     LMAINGDTNVASRGGEGGLRWLQREAQTLLQKGGIRTPADLDYLRQFDRECIERNLSP
                     GGSADLLILTWFLAQI"
     gene            5403..6866
                     /gene="citT"
                     /locus_tag="ACHOSC_RS13550"
                     /old_locus_tag="pgaptmp_002710"
     CDS             5403..6866
                     /gene="citT"
                     /locus_tag="ACHOSC_RS13550"
                     /old_locus_tag="pgaptmp_002710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="citrate/succinate antiporter CitT"
                     /protein_id="HGU4548624.1"
                     /translation="MSLAKDNIWKLLAPLVVMGVMFLIPVPDGMPPQAWHYFAVFVAM
                     IVGMILEPIPATAISFIAVTICVIGSNYLLFDAKELADPAFNAQKQALKWGLAGFSST
                     TVWLVFGAFIFALGYEVSGLGRRIALFLVKFMGKRTLTLGYAIVIIDILLAPFTPSNT
                     ARTGGTVFPVIKNLPPLFKSFPNDPSARRIGGYLMWMMVISTSLSSSMFVTGAAPNVL
                     GLEFVSKIAGIQISWLQWFLCFLPVGVILLIIAPWLSYVLYKPEITHSEEVATWAGDE
                     LKTMGALTRREWTLIGLVLLSLGLWVFGSEVINATAVGLLAVSLMLALHVVPWKDITR
                     YNSAWNTLVNLATLVVMANGLTRSGFIDWFAGTMSTHLEGFSPNATVIVLVLVFYFAH
                     YLFASLSAHTATMLPVILAVGKGIPGVPMEQLCILLVLSIGIMGCLTPYATGPGVIIY
                     GCGYVKSKDYWRLGAIFGVIYISMLLLVGWPILAMWN"
     gene            6980..7786
                     /gene="rna"
                     /locus_tag="ACHOSC_RS13555"
                     /old_locus_tag="pgaptmp_002711"
     CDS             6980..7786
                     /gene="rna"
                     /locus_tag="ACHOSC_RS13555"
                     /old_locus_tag="pgaptmp_002711"
                     /EC_number="4.6.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease I"
                     /protein_id="HGU4548625.1"
                     /translation="MKAFWRNAALLAVSLLPFSSANALALQAKQYGDFDRYVLALSWQ
                     TGFCQSQHDRNRNERDECRLQTETTNKADFLTVHGLWPGLPKSVAARGVDERRWMRFG
                     CATRPIPNLPEAHASRMCSSPETGLSLETAAKLSEVMPGAGGRSCLERYEYAKHGACF
                     GFDPDAYFGTMVRLNQEIKESEAGKFLADNYGKTVSRRDFDAAFAKSWGKENVKAVKL
                     TCQGNPAYLTEIQISIKADAINAPLSANSFLPQPHPGNCGKTFVIDKVGY"
     gene            8016..8426
                     /gene="rnk"
                     /locus_tag="ACHOSC_RS13560"
                     /old_locus_tag="pgaptmp_002712"
     CDS             8016..8426
                     /gene="rnk"
                     /locus_tag="ACHOSC_RS13560"
                     /old_locus_tag="pgaptmp_002712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0070063 - RNA polymerase binding [Evidence
                     IEA];
                     GO_process: GO:0032784 - regulation of DNA-templated
                     transcription elongation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside diphosphate kinase regulator"
                     /protein_id="HGU4548626.1"
                     /translation="MSRPTIIINDLDAERIDILLEQPAYAGLPIADALNAELDRAQMC
                     SPEEMPHDVVTMNSRVKFRNLSDGEVRVRTLVYPAKMTDSNTQLSVMAPVGAALLGLR
                     VGDSIHWELPGGVATHLEVLELEYQPEAAGDYLL"
     gene            8564..8653
                     /gene="yldA"
                     /locus_tag="ACHOSC_RS13565"
                     /old_locus_tag="pgaptmp_002713"
     CDS             8564..8653
                     /gene="yldA"
                     /locus_tag="ACHOSC_RS13565"
                     /old_locus_tag="pgaptmp_002713"
                     /inference="COORDINATES: protein motif:HMM:NF041476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="small membrane protein YldA"
                     /protein_id="HGU4548627.1"
                     /translation="MAEAFYILIGFLIMAAIIVMAVLYLENHS"
     gene            complement(8657..9895)
                     /gene="ybdR"
                     /locus_tag="ACHOSC_RS13570"
                     /old_locus_tag="pgaptmp_002714"
     CDS             complement(8657..9895)
                     /gene="ybdR"
                     /locus_tag="ACHOSC_RS13570"
                     /old_locus_tag="pgaptmp_002714"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004024 - alcohol dehydrogenase (NAD+)
                     activity, zinc-dependent [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0030554 - adenyl nucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-dependent alcohol dehydrogenase"
                     /protein_id="HGU4548628.1"
                     /translation="MKALTYHGPHHVQVENVPDPGIEQADDIILRITATAICGSDLHL
                     YRGKIPQVKHGDIFGHEFMGEVVETGKDVKNLQKGDRVVIPFVIACGDCFFCRMQQYA
                     ACENTNAGKGAALNKKQIPAPAALFSYSHLYGGVPGGQAEYVRVPKGNVGPFKVPPLL
                     SDDKALFLSDILPTAWQAAKNAQIQQGSSVAVYGAGPVGLLTIACARLLGAEQIFVVD
                     HHPYRLRFAADRYGAIPINFDEDSDPAQSIIEQTAGHRGVDAVIDAVGFEAKGSTTET
                     VLTNLKLEGSSGKALRQCIAAVRRGGIVSVPGVYAGFIHGFLFGDAFDKGLTFKMGQT
                     HVHAWLGELLPLIEKGLLKPEEIVTHYMPFEEAARGYEIFEKREEECRKVILVPGAQS
                     AEAAQKAVSGLVNAMPGGTI"
     gene            10116..10544
                     /gene="uspG"
                     /locus_tag="ACHOSC_RS13575"
                     /old_locus_tag="pgaptmp_002715"
     CDS             10116..10544
                     /gene="uspG"
                     /locus_tag="ACHOSC_RS13575"
                     /old_locus_tag="pgaptmp_002715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein UspG"
                     /protein_id="HGU4548629.1"
                     /translation="MYKTIIMPVDVFEMELSDKAVRHAEFLAQDDGVIHLLHVLPGSA
                     SLSLHRFAADVRRFEEHLQHEAEERLQTMVSHFTIDPSRIKQHVRFGSVRDEVNELAE
                     ELGADVVVIGSRNPSISTHLLGSNASSVIRHANLPVLVVR"
     gene            complement(10665..12230)
                     /gene="ahpF"
                     /locus_tag="ACHOSC_RS13580"
                     /old_locus_tag="pgaptmp_002716"
     CDS             complement(10665..12230)
                     /gene="ahpF"
                     /locus_tag="ACHOSC_RS13580"
                     /old_locus_tag="pgaptmp_002716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017455864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008785 - alkyl hydroperoxide reductase
                     activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0000302 - response to reactive oxygen
                     species [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alkyl hydroperoxide reductase subunit F"
                     /protein_id="HGU4548630.1"
                     /translation="MLDTNMKTQLKAYLEKLTKPVELIATLDDSAKSAEIKELLAEIA
                     ELSDKVTFKEDNSLPVRKPSFLITNPGSNQGPRFAGSPLGHEFTSLVLALLWTGGHPS
                     KEAQSLLEQIRHIDGDFEFETYYSLSCHNCPDVVQALNLMSVLNPRIKHTAIDGGTFQ
                     NEITDRNVMGVPAVFVNGKEFGQGRMTLTEIVAKIDTGAEKRAAEELNKRDAYDVLIV
                     GSGPAGAAAAIYSARKGIRTGLMGERFGGQILDTVDIENYISVPKTEGQKLAGALKVH
                     VDEYDVDVIDSQSASKLIPAAVEGGLHQIETASGAVLKARSIIVATGAKWRNMNVPGE
                     DQYRTKGVTYCPHCDGPLFKGKRVAVIGGGNSGVEAAIDLAGIVEHVTLLEFAPEMKA
                     DQVLQDKLRSLKNVDIILNAQTTEVKGDGSKVVGLEYRDRVSGDIHNIELAGIFVQIG
                     LLPNTNWLEGAVERNRMGEIIIDAKCETNVKGVFAAGDCTTVPYKQIIIATGEGAKAS
                     LSAFDYLIRTKTA"
     gene            complement(12401..12964)
                     /gene="ahpC"
                     /locus_tag="ACHOSC_RS13585"
                     /old_locus_tag="pgaptmp_002717"
     CDS             complement(12401..12964)
                     /gene="ahpC"
                     /locus_tag="ACHOSC_RS13585"
                     /old_locus_tag="pgaptmp_002717"
                     /EC_number="1.11.1.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003858764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0102039 - NADH-dependent peroxiredoxin
                     activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alkyl hydroperoxide reductase subunit C"
                     /protein_id="HGU4548631.1"
                     /translation="MSLINTKIKPFKNQAFKNGEFIEITEKDTEGRWSVFFFYPADFT
                     FVCPTELGDVADHYEELQKLGVDVYAVSTDTHFTHKAWHSSSETIAKIKYAMIGDPTG
                     ALTRNFDNMREDEGLADRATFVVDPQGIIQAIEVTAEGIGRDASDLLRKIKAAQYVAS
                     HPGEVCPAKWKEGEATLAPSLDLVGKI"
     gene            13336..14082
                     /gene="dsbG"
                     /locus_tag="ACHOSC_RS13590"
                     /old_locus_tag="pgaptmp_002718"
     CDS             13336..14082
                     /gene="dsbG"
                     /locus_tag="ACHOSC_RS13590"
                     /old_locus_tag="pgaptmp_002718"
                     /EC_number="1.8.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308670.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiol:disulfide interchange protein DsbG"
                     /protein_id="HGU4548632.1"
                     /translation="MLKKILLLALLPAIAFAEELPAPVKAIEKQGITIIKTFDAPGGM
                     KGYLGKYQDMGVTIYLTPDGKHAISGYMYNEKGENLSNTLIEKEIYAPAGREMWQRME
                     QSHWLLDGKKDAPVIVYVFADPFCPYCKQFWQQARPWVDSGKVQLRTLLVGVIKPESP
                     ATAAAILASKDPAKTWQEYESSGGKLKLNVPANVSTEQMKVLSANEKLMDDLGANVTP
                     AIYYMSKENTLQQAVGLPDQKTLNIIMGNK"
BASE COUNT         3393 a         3759 c         3579 g         3506 t
ORIGIN      
        1 gagaaaaaga tatacctcga tcacccccct ttctcccaag tgaaaataaa aggttatcag
       61 tttgcaacat tgaacaacat tcgttgcaaa tcgataacaa catgcacctt caggatacta
      121 tttattatgt tcggcaatga tattttcacc cgcgtaaaac gttcagaaaa taaaaaaatg
      181 gcggaaatcg cccaattcct gcatgaaaat gatttgagcg ttgacaccac agtcgaagta
      241 tttattaccg taacccgcga tgaaaagctt atcgcgtgcg gtggaattgc cggaaatatt
      301 attaaatgcg ttgctatcag tgaatccgtt cgcggtgaag gactggcgct gacattagcc
      361 actgaactga taaacctcgc ctatgagcgg cacagcacgc atctgtttat ttataccaaa
      421 accgaatacg aggcgctgtt ccgccagtgc ggtttttcca cgctgaccag cgtacccggc
      481 gtgatggtgc tgatggaaaa cagcgccacg cggctgaaac gctatgccga atcgctgaaa
      541 aaatttcgtc atccagggaa caagattggc tgcattgtga tgaacgccaa tcccttcacg
      601 aatggtcacc gttatctgat tcaacaagct gcggcacagt gcgactggtt gcatctgttt
      661 ttagtcaaag aagattcttc acgcttcccc tatgaagacc ggttggatct ggtgttaaaa
      721 ggcaccgccg atattccacg cctgactgtg catcgcggct ccgaatacat catctcccgc
      781 gctacgttcc cttgctactt cattaaagaa cagagcgtca ttaaccattg ttacaccgaa
      841 attgatctga agattttccg tcagtacctc gctcccgcgc tgggtgtaac tcaccgcttt
      901 gtcggtactg aacccttttg tcgcgttacc gcccagtaca accaggatat gcgctactgg
      961 ctggaaacgc cgactatctc cgcaccgccc atcgaactgg ttgaaattga gcggctgcgt
     1021 taccaggaga tgccgatatc cgcttcccgg gtacgtcaac tgctggcgaa aaacgatctc
     1081 acggctatcg cgccgctggt ccctgcagtc acgctgcatt atttgcagaa cctgcttgag
     1141 cactcccgcc aggacgcggc agctcgtcaa aagacccccg catgagaaac aggtgaaaaa
     1201 tgaaaataaa ccagcccgcc gttgcaggca cccttgagtc tggggatgtg atgatacgca
     1261 tcgccccact cgatacgcag gatatcgacc tgcaaatcaa tagcagcgtt gagaaacagt
     1321 ttggcgatgc aattcgcacc accattctgg acgttctcgc ccgctacaac gtgcgcggcg
     1381 tacagctgaa tgtcgatgac aaaggcgcac tggactgcat tttacgtgca cgactggaag
     1441 ccctgctggc acgcgccagc ggtatcccgg ctctgccatg ggaggattgc caatgatttc
     1501 cgcttcgctg caacaacgta aaactcgcac ccgccgcagc atgttgtttg tgcctggtgc
     1561 caatgccgcg atggtcagca actccttcat ctacccggct gatgccctga tgtttgacct
     1621 cgaagactcc gtggcattac gtgaaaaaga caccgcccgc cgcatggttt atcacgcgct
     1681 gcaacatccg ctgtatcgcg atattgaaac cattgtgcgt gtcaacgcgc tggattccga
     1741 atggggtgtt aacgacctgg aagccgtcgt tcgcggtggt gcggacgttg tgcgtctgcc
     1801 gaaaaccgat accgctcagg atgttctgga tattgaaaaa gagatcctgc gtatcgaaaa
     1861 agcctgtggt cgtgaacccg gcagcaccgg cctgctggcg gcgattgaat ctccgctggg
     1921 cattacccgc gcagtggaaa tcgctcacgc ttccgagcgt ttgatcggta tcgccctcgg
     1981 tgcagaagac tatgtgcgca acctgcgtac agaacgctcc ccggaaggaa ctgaactgct
     2041 gttcgcacgc tgttccattt tgcaggccgc gcgctctgcg ggtattcagg cgttcgatac
     2101 cgtctattcc gacgctaaca acgaagccgg attcctgcaa gaagccgccc acatcaaaca
     2161 gctgggcttt gacggcaaat cgctgatcaa cccgcgtcag attgatctgt tgcacaacct
     2221 ctacgcacca acccagaaag aagtggatca cgcccgccgc gtcgtagaag ccgctgaagc
     2281 cgccgctcgc gaaggcctcg gcgtggtttc cctgaacggc aagatggtgg acggtccggt
     2341 tatcgatcgc gcccgtctgg tgctctctcg tgcagaactt tccggcatcc gcgaagaata
     2401 aggcaatcaa aatgacgcag aaaattgaac aatctcaacg acaagaacgg gtagcggcct
     2461 ggaatcgtcg cgctgaatgc gatcttgccg ctttccagaa ctcgccaaag caaacctacc
     2521 aggctgaaaa agcgcgcgat cgcaaactgt gcgccaacct ggaagaagcg attcgtcgct
     2581 ctggtttaca ggacggcatg acggtttcct tccatcacgc tttccgtggc ggtgacctga
     2641 ccgtcaatat ggtgatggac gtcatcgcga agatgggctt taaaaacctg accctggcgt
     2701 ccagctccct gagtgattgc catgcgccgt tggtagaaca cattcgccag ggcgtggtta
     2761 cccgcattta tacctccggc ctgcgcggtc cactggcgga agagatctcc cgtggtctgc
     2821 tggcagaacc ggtgcaaatc cactctcacg gcggtcgcgt gcatctggta cagagcggcg
     2881 aactgaatat cgacgtggct ttcctcggcg tcccgtcctg cgatgaattc ggtaatgcca
     2941 acggctacac cggtaaagcc tgctgcggct ccctcggcta tgcaatggtt gatgccgaca
     3001 acgcaaagca ggtcgtgatg cttaccgaag aactgctgcc ttatccgcat aatccggcaa
     3061 gcattgagca agatcaggtt gatttgatcg tcaaagttga ccgcgttggc gatgctgcaa
     3121 aaatcggcgc tggcgcgacc cgtatgacca ctaacccgcg cgaactgctt attgcccgta
     3181 gcgctgcgga tgtgattgtc aactcaggct acttcaaaga aggtttctcc atgcaaaccg
     3241 gcaccggcgg cgcatcgctg gcggtaaccc gtttcctgga agacaaaatg cgtagccgcg
     3301 atattcgcgc cgacttcgcc cttggcggta ttaccgcgac gatggttgac ctgcacgaaa
     3361 aaggtctgat ccgcaaactg ctggatgtgc agagctttga cagccatgct gcgcaatcgc
     3421 tggcccgtaa ccccaatcac atcgaaatca gcgccaacca gtacgctaac tggggttcga
     3481 aaggcgcatc ggttgatcgt ctcgacgtgg tggtactgag cgcgctggaa attgacaccc
     3541 agttcaacgt taacgtgctg accggctctg acggcgtact gcgtggtgct tccggtggtc
     3601 actgcgatac cgcgattgcc tctgcgcttt ccatcatcgt cgcgccgctg gtacgcggtc
     3661 gtattccgac tctggtggat aacgtgctga cctgcatcac cccgggctcc agtgtcgata
     3721 ttctggtcac agaccacggt atcgcagtta acccggcacg tccggaactg gcagaacgtc
     3781 tgcaggaagc gggcattaaa gtggtttcca ttgagtggct gcgcgaacgt gcgcgtctgc
     3841 tgaccggtga accacagccg attgaattca ccgaccgcgt cgttgccgtt gtgcgttacc
     3901 gcgatggctc ggtgatcgat gttgtgcatc aggtgaagga ataagccatg cacctgcttc
     3961 ctgaactcgc cagccaccat gcggtgtcaa ttcccgagct gctcgtcagc cgggatgaaa
     4021 ggcaagcacg gcaacacgtc tggctcaagc gccatcctgt tccactggtc tcctttaccg
     4081 tggttgcgcc tgggccgatt aaagacagcg aggtcacacg ccgaattttt aatcatggcg
     4141 tgacagcctt gcgtgcctta gccgcaaaac agggctggca aattcaggag caggctgcac
     4201 tggtttccgc cagcgggccg gagggcatgt tgagcattgc cgccccggct cgcgacctca
     4261 agctcgccac cattgagctt gaacatagtc atcctctcgg gcggttatgg gatatcgatg
     4321 tcctgacgcc cgaaggcgaa attctctccc gccgcgacta ttcactgccg cctcgccgct
     4381 gcctgttgtg cgagcaaagc gcagccgtct gcgcgcgtgg aaaaacccat caactgaccg
     4441 atttactcaa ccgcatggag gcactgctga acgatgtcga tgcctgcaac gtcaactaaa
     4501 accacaaagc ttgcgacgtc attaatcgat gagtacgccc tgctgggctg gcgcgccatg
     4561 ctgactgaag tcaatctgtc accgaaacca ggcctcgtgg atcgcattaa ctgcggtgcg
     4621 cacaaagata tggcgctgga agatttccac cgcagcgcgc tggcgattca gggctggtta
     4681 ccccgtttca ttgaatttgg tgcctgtagt gcagaaatgg caccggaagc ggtactccac
     4741 ggattacgcc caattggtat ggcttgcgaa ggtgatatgt tccgcgccac tgcgggcgta
     4801 aacacgcata aaggcagcat tttttcttta gggctgctat gtgcggcaat tggccgtttg
     4861 cttcagctca accagccggt aacgccaaca accgtttgtt ctacggcggc aagtttctgc
     4921 cgtggtctga ccgatcgcga actgcgtacc aataattcac aactgacggc aggtcaacgg
     4981 ttgtaccagc agcttggcct taccggcgca cgcggtgaag ccgaagcggg ttatccactg
     5041 gtgatcaacc acgccttgcc gcattacctc actctgctgg atcaggggtt agatcctgaa
     5101 ctggcattgc tcgatacctt gctcctactg atggcgatca acggcgatac caacgttgct
     5161 tcgcgcggtg gcgagggggg cctgcgctgg ctacagcgcg aggcgcaaac attattgcaa
     5221 aaagggggca ttcgaacccc cgccgatctc gattatctcc ggcagttcga cagggagtgt
     5281 atcgaacgaa atctcagtcc aggcggcagt gccgacctgc tgatccttac ctggttttta
     5341 gcacagattt aattatttaa gcacttgata aatttgaaaa tattaatttt cggagaaccc
     5401 gtatgtcttt agcaaaagat aatatatgga aactattggc cccactggtg gtgatgggtg
     5461 tcatgtttct tatccctgtc cccgacggta tgccaccaca ggcatggcat tacttcgctg
     5521 tgtttgtggc aatgattgtc ggcatgatcc tcgagccaat tccggcaaca gcgatcagtt
     5581 ttattgcggt tactatttgc gttattggca gtaattacct gctctttgat gccaaagaat
     5641 tagctgaccc agcgtttaat gcgcaaaaac aggcgctgaa atggggtctg gctggttttt
     5701 ccagcaccac ggtatggctg gtatttggcg catttatttt tgcattaggg tatgaagttt
     5761 ccgggttagg tcgtcgcatt gcccttttcc tggtgaaatt catgggcaaa cgcacgctga
     5821 cgttgggtta tgcgattgtc attatcgaca ttctgctggc accgtttaca ccgtccaaca
     5881 ccgcgcgtac cgggggtacg gtttttccgg tcattaaaaa cctgccgccg ttgtttaaat
     5941 cattccccaa cgatccgtcc gcgcgtcgta ttggcggcta tttgatgtgg atgatggtca
     6001 ttagtaccag tctgagttcg tccatgtttg tcaccggtgc ggcaccaaac gtgctgggtc
     6061 tggagttcgt cagcaaaatt gccggtatcc agattagctg gttgcagtgg ttcctctgct
     6121 tcctgccggt tggggttatc ttgcttatca ttgcgccgtg gctttcctac gtgctgtaca
     6181 aaccggaaat cacacacagt gaagaagtgg caacctgggc gggtgatgaa ctgaaaacca
     6241 tgggtgcgct gacacgcaga gagtggacac tgattggcct tgtattgctc agcttaggtt
     6301 tgtgggtttt tggcagcgaa gtcattaatg ctactgcagt tggtctgctg gcagtttcgc
     6361 taatgctggc cctgcacgtt gtgccttgga aagatattac ccgctataac agcgcatgga
     6421 acacgctggt caacctggca actctggttg tgatggctaa cggcctgact cgttctggct
     6481 ttattgactg gttcgccggt accatgagta cgcacctgga aggattctca ccaaacgcaa
     6541 cggtgattgt actggttctg gtgttctact ttgcacacta cctgtttgcc agcctgtctg
     6601 cgcacaccgc aaccatgctg ccggttattc tggccgtcgg taaaggtatt ccgggcgtac
     6661 caatggaaca actgtgtatc ctgctggtgc tgtctatcgg tatcatgggc tgtctgacgc
     6721 cgtatgcaac cggtcctggg gtgattattt acggctgtgg ctatgtgaaa tcaaaagatt
     6781 actggcgtct tggcgcaatc ttcggggtga tttacatctc tatgttgctg ttggttggct
     6841 ggccgattct cgccatgtgg aactaatacg ttcgccaaac gctgaataaa tcatcacgcc
     6901 cgccaggtaa gcccacctgg cgggcttttt tatgatttaa tagatagtat tcagtgtatg
     6961 tatgagttcc acacccatta tgaaagcatt ctggcgtaac gccgcgttgc tcgcggtttc
     7021 tctgcttccc ttctcttctg ccaacgcctt agcgttgcag gcaaaacagt atggcgattt
     7081 tgatcgctat gtcctggccc tctcctggca aaccggattt tgccagagtc aacacgatcg
     7141 aaatcgtaac gaacgagatg aatgtcgcct gcaaaccgaa acgaccaaca aagctgattt
     7201 tctgaccgta catggtctgt ggccaggatt gcctaaatcg gttgctgccc gtggtgttga
     7261 tgaacgccgc tggatgcgct tcggttgcgc tactcgccca atcccgaatc taccagaagc
     7321 gcacgccagc cggatgtgtt catcgccgga aaccggatta tcactggaaa cggccgctaa
     7381 actaagtgaa gtcatgccag gagctggcgg acgttcctgc ctggaacgct acgaatatgc
     7441 caaacacggt gcctgctttg gttttgatcc ggacgcatac ttcggtacga tggtacgcct
     7501 gaatcaagaa attaaagaga gcgaagccgg aaaattcctt gcggataatt acggtaaaac
     7561 agtgagccgc cgtgactttg acgccgcctt tgccaaaagc tggggaaaag agaacgtgaa
     7621 agcagttaag ctaacgtgcc agggtaaccc tgcgtatttg actgaaattc agatctcgat
     7681 caaagctgac gcaatcaacg ctccgctttc tgcaaactca ttcttgccac aaccacaccc
     7741 aggcaactgt ggcaaaacct ttgtgattga taaagtgggt tattaatcat ctgatttaca
     7801 aaacacgcta attcgtcttc cataaaccgt gacgattaag aattatacat aatgcgctgc
     7861 attgaggaat tccctgaatg tgacgcaaat cacttcaaag agtgagcaac agtcagtatc
     7921 atcatgtgag ttaaaccctc gccgcctgac ggtgagggtt ttcttttggg tatgtttatc
     7981 tgcgacactc gcagtaccga cgacatggag taaaaatgtc cagaccaact atcatcatta
     8041 acgacctgga tgccgaacgc atcgatattc tgctggagca acccgcctat gctggtttgc
     8101 caatcgccga cgcgttaaac gcagagttgg atcgcgccca aatgtgttcg ccagaagaga
     8161 tgccacacga cgtggtgaca atgaacagcc gggttaaatt ccgcaatctt agcgatggcg
     8221 aagtgcgtgt gcgcacgctg gtgtatccgg caaaaatgac cgatagcaat actcagcttt
     8281 ccgttatggc tccggtaggt gccgcactgc tggggctgcg cgttggcgat tccattcact
     8341 gggaacttcc gggcggcgtt gcaacccacc ttgaagtgct ggaactcgaa taccagccag
     8401 aagctgctgg cgactacctg ctttaatccc atctgcacaa gcgataactc gctttgccca
     8461 gaggatgcac tcgcatcctc tttacgcaaa atcagcaatt tttcctggtt ctcactgttc
     8521 acttttgctg caaaattatt ccgtttcgtt tgtgggaata accatggcag aagcatttta
     8581 catcctgata ggatttctga ttatggcggc gattatcgtc atggctgtcc tttacctcga
     8641 aaaccactcc tgacgatcat attgttcccc ccggcatcgc attcaccaga cctgaaaccg
     8701 ccttctgcgc cgcctctgcg ctttgtgcac cgggtaccag aatcacctta cggcactcct
     8761 cttcacgttt ttcgaaaatc tcatatcccc gggcggcctc ttcaaacggc atatagtggg
     8821 taacaatttc ttctggtttc agtaatcctt tctcaattaa cggtaataat tctcccagcc
     8881 atgcgtgaac gtgggtctgt cccattttaa acgtcaaccc tttatcaaag gcgtcgccaa
     8941 acaggaaacc gtgaataaat ccagcgtaga cgcccggtac gctaacaatg ccgccacgcc
     9001 tgaccgccgc aatacactgg cgcaacgctt taccgctgct gccctccagt ttcaggttag
     9061 tcagcaccgt ttccgtggtg ctgcctttcg cttcaaaacc gacggcgtct attactgcat
     9121 ccacgccccg gtgacctgcc gtttgttcaa taattgactg tgccggatcg ctatcttcat
     9181 cgaaattaat cgggatcgcg ccgtagcggt cagcggcgaa acgcaagcgg tagggatgat
     9241 gatcaacaac aaaaatctgt tccgcaccga gcaaccgtgc acaggcgatt gtcaacaatc
     9301 ccactggacc ggcaccatag actgcaacgc ttgaaccttg ttggatctgc gcattttttg
     9361 ctgcctgcca tgccgttggc agaatatcag aaaggaaaag ggctttatca tctgaaagca
     9421 aaggcggtac tttaaacggc cccacattcc ctttagggac gcggacatat tccgcctgtc
     9481 caccaggaac gccaccatac aggtgactat aactaaacaa tgccgcggga gctggtatct
     9541 gttttttatt gagcgcagcg cctttacccg cattggtatt ttcgcaggcg gcatattgct
     9601 gcattcgaca gaaaaaacag tcgccacaag caatgacaaa cggaattacc acccggtcgc
     9661 ctttttgcaa attttttacg tcctttccgg tttcaacgac ttcccccata aattcatgac
     9721 caaaaatatc gccatgttta acctggggta ttttgcctcg ataaagatgg aggtcagagc
     9781 cacagatcgc cgttgccgta atacgcagaa taatatcatc tgcctgttca atgcccggat
     9841 cgggaacatt ttctacctga acgtgatgtg ggccgtgata agtcaatgct ttcatgcaac
     9901 ctccagagac agttcaaaaa agagtcagtc tttaagggta gctatcacag cgggaaaacg
     9961 ggcggtaccg caggttttgg gagaattcct ggtgttcagt gagaatatat taatcacaga
    10021 aaccgttcgt aaggaaaaag aaccagacaa atatcaggga taaatattat aatcatggcg
    10081 aatgatttgt tcatgattaa cagggagaaa gggttatgta taagacaatc attatgccag
    10141 ttgatgtatt tgaaatggaa ttgagcgaca aagctgttcg ccacgctgaa ttcctcgccc
    10201 aggatgacgg agttattcat ctacttcacg tactacccgg gtcagccagc ctgagcctgc
    10261 accgttttgc cgctgatgtg cgtcgttttg aagagcatct gcaacatgaa gcagaagaac
    10321 gtctgcaaac gatggtcagc cacttcacca tcgatccttc ccgcattaaa caacatgtcc
    10381 gttttggtag cgtgcgggat gaagtcaatg agttggcaga agaactgggg gctgatgttg
    10441 tagttattgg ttctcgcaac ccatcgattt cgacccatct gttaggttct aacgcctcga
    10501 gcgtgatccg ccatgccaat ctgccggtgc tggttgtgcg ttaattgcca gaataaatat
    10561 cccgccctgc ccggagccat cccggcaggg ctttttaatg ccataaaaaa gccgccaggt
    10621 ttgaccccgg cggctaagca attgcaggtg aatcttactt cttcttatgc agttttggtg
    10681 cgaatcaggt agtcaaaagc actcagagag gctttggcac cttcgcctgt ggcgatgata
    10741 atctgcttgt acggaaccgt cgtacagtca cccgctgcga acacgccttt gacgttggtt
    10801 tcgcatttcg catcaatgat aatctcaccc atgcggttac gttcgactgc gccttccagc
    10861 cagttggtgt tcggcagcag accaatctgg acgaaaatac cggccagttc gatgttgtga
    10921 atatcgccgc tgacacgatc tcgatattcc agaccaacga ctttgctgcc gtcgcctttc
    10981 acttccgtgg tttgcgcatt cagaataatg tcgacgtttt tcaggctgcg cagtttgtcc
    11041 tgcagaacct ggtcggcttt catttctggc gcaaattcga gcagcgttac gtgctcaacg
    11101 atacccgcca ggtcaattgc cgcttccacg ccggagttac cgccgccgat aaccgctacg
    11161 cgtttgcctt taaacagcgg gccgtcgcag tgcgggcagt aggtcacgcc tttggtgcga
    11221 tactgatctt cgccaggaac gttcatgttg cgccattttg caccggtcgc cacgataatg
    11281 ctgcgtgctt tcagtaccgc gccagaagct gtttcaatct gatgcagacc accttcaact
    11341 gctgctggga tcagtttgct ggcgctctgg ctgtcgatca catcaacgtc gtattcatca
    11401 acgtgaactt tcagtgcgcc tgccagcttc tgcccttcag tcttcggtac agaaatgtag
    11461 ttttcgatat caacggtatc gaggatctga ccaccaaaac gttcgcccat cagaccggta
    11521 cggatgcctt tacgtgcgga gtaaattgct gcagctgcac ccgccgggcc ggaaccaacg
    11581 attaatacgt cataagcatc acgcttgttc agctcttctg ccgcacgttt ttccgcgccc
    11641 gtatcaattt tggcaacgat ttcagtcaac gtcatgcggc cctgaccaaa ctctttcccg
    11701 tttacgaaca ctgccggaac gcccatcacg ttgcgatcgg tgatttcgtt ctggaaggtg
    11761 ccgccgtcaa ttgcagtgtg cttgatgcgc gggttcagta cgctcatcag gttcagcgcc
    11821 tgcaccacgt ccgggcagtt atggcaagag agcgagtaat aggtttcgaa ttcaaaatca
    11881 ccgtcaatat ggcgaatctg ctccagcaga gactgcgctt ctttcgacgg atgaccaccg
    11941 gtccatagca acgccagtac cagcgaggtg aactcgtggc ccagcgggga gcctgcaaaa
    12001 cgtggcccct ggttggaacc tgggttggtg atcaggaaag acggcttacg caccggcaag
    12061 ctgttatctt ctttaaaggt gactttgtct gacagttctg cgatttcagc caacagttcc
    12121 ttgatttctg ccgatttagc gctgtcatcc agcgtggcaa ttaactcaac aggcttggtc
    12181 aatttctcaa ggtaagcctt gagttgagtt ttcatatttg tgtcgagcat gtttatctcc
    12241 tgggctttaa acatcatcat gcaagcggcc tctgcggcta cctggatgca gcttgcatca
    12301 tggtgcgggg aagaaaatcg ggtgccgcag cacccgaaga attaggtgaa tttccgaagt
    12361 atttcgcgcc ggagttggta gcgcgaaaga cgaaggaaat ttagatttta ccaaccaggt
    12421 ccagagacgg agccagagtt gcttcacctt ctttccattt agccgggcaa acttcacctg
    12481 ggtgagaagc tacgtactgt gctgctttga ttttacgcag caggtcagac gcgtcacggc
    12541 caatgccttc agcggtaact tcgattgcct ggatgatacc ctgcgggtca acaacgaagg
    12601 tcgcacggtc agccagacct tcatcttcac gcatgttgtc gaagttacgg gtcagggcgc
    12661 cagtcgggtc gccgatcatc gcatatttga ttttagcgat ggtttcagag ctgctgtgcc
    12721 atgctttgtg ggtgaagtga gtatcggtag atactgcgta tacgtctacg cccagtttct
    12781 gcagttcttc gtagtggtca gcaacgtcac ccagttcggt cgggcatacg aaagtaaagt
    12841 cagccgggta gaagaagaag acgctccagc ggccttcggt atctttttcg gtgatttcga
    12901 tgaattcgcc gtttttgaat gcctggtttt taaaaggttt aattttggtg ttaatcaagg
    12961 acatctatac ttcctccgtg ttttcgatga gatgtaaggt aaccaatttt tgccgattcg
    13021 gctaatgcgt ttccattatc aaatcgataa gttttacctt acaaccttcg taagacaact
    13081 ttgtgaactt tgcaccgcca gcggcgataa aaagtaaaat ggcccccttt ccaggtgacc
    13141 gtgtaatctg aactaccctt tggtatcttc agtgcctgcc tgcaggctga ggtgttgccc
    13201 ttacaaatgc aacaacgaca tggattacaa caccctcata aacaaagggc aatcacctga
    13261 tctaagctct tacctatgac agtgataggt tatgcctttt actcgacttt tgcactgact
    13321 gaaaaggaca aattaatgtt aaaaaagata cttttactgg ctctgcttcc tgcaatcgcc
    13381 ttcgcagagg aacttcctgc tccagtaaaa gcgattgaaa aacagggcat tacaatcatc
    13441 aaaacattcg atgcccccgg aggaatgaaa ggttatctcg gaaagtatca ggatatgggc
    13501 gtcaccatct acctgactcc agatggtaag cacgctatct ctggttacat gtacaacgag
    13561 aaaggtgaaa acctgagtaa cacacttatc gaaaaagaaa tttacgcacc agccggacgc
    13621 gaaatgtggc aacggatgga acaatcccac tggctcctcg acggtaaaaa agatgcgccg
    13681 gtcattgtct acgtgttcgc cgatccgttc tgcccatatt gtaaacagtt ctggcagcag
    13741 gcgcgcccgt gggtagattc tggcaaagtg caattaagaa cattgttggt tggggttatc
    13801 aagccagaaa gcccggcgac agcagcggca attcttgcct ccaaagatcc cgcaaaaacc
    13861 tggcaagaat atgaatcctc tggtggcaag cttaagctaa acgtgcctgc aaacgtaagt
    13921 acagaacaaa tgaaagtgtt aagcgccaac gagaaactca tggacgatct gggggcaaat
    13981 gtcacaccag cgatctatta catgagtaag gaaaatacgc tgcaacaggc cgtggggttg
    14041 cccgatcaga aaacgcttaa tatcattatg gggaataaat aaaagataat tcatggatgg
    14101 ttgaaataca tccctgcttt tcaaccatcc taaaaaagca tgaattgctc gctctgccct
    14161 aagtggctat gctattttag aaaaacttaa tcctgagtta agcaaaaaaa tataatcaat
    14221 aaaaatatat acgcaaa
//
LOCUS       DAZKHF020000114        14025 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2201S31241
            SAMN43267800-rid25106413.denovo.115, whole genome shotgun sequence.
ACCESSION   DAZKHF020000114 DAZKHF020000000
VERSION     DAZKHF020000114.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267800
            Sequence Read Archive: SRR30307263
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 14025)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14025)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 14025)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350581.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350581.2
            Genome Coverage       :: 151x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:21:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,652
            CDSs (total)                      :: 5,557
            Genes (coding)                    :: 5,320
            CDSs (with protein)               :: 5,320
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 237
            CDSs (without protein)            :: 237
            Pseudo Genes (ambiguous residues) :: 0 of 237
            Pseudo Genes (frameshifted)       :: 102 of 237
            Pseudo Genes (incomplete)         :: 130 of 237
            Pseudo Genes (internal stop)      :: 52 of 237
            Pseudo Genes (multiple problems)  :: 41 of 237
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14025
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267800-rid25106413.denovo.115"
                     /serotype="O 26:H11"
                     /isolate="2201S31241"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-01"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            34..123
                     /locus_tag="ACHOSC_RS13595"
                     /old_locus_tag="pgaptmp_002719"
                     /pseudo
     CDS             34..123
                     /locus_tag="ACHOSC_RS13595"
                     /old_locus_tag="pgaptmp_002719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311139.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1175 family protein"
     gene            147..4661
                     /locus_tag="ACHOSC_RS13600"
                     /old_locus_tag="pgaptmp_002720"
     CDS             147..4661
                     /locus_tag="ACHOSC_RS13600"
                     /old_locus_tag="pgaptmp_002720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000536590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004866 - endopeptidase inhibitor activity
                     [Evidence IEA];
                     GO_process: GO:0010951 - negative regulation of
                     endopeptidase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-2-macroglobulin"
                     /protein_id="HGU4548633.1"
                     /translation="MGTGLANADDSLPSSNYAPPAGGTFFLLADSSFSSSEEAKVRLE
                     APGRDYRRYQMEEYGGVDVRLYRIPDPMAFLRQQKNLHRIVVQPQYLGDGLNNTLTWL
                     WDNWYGKSRRVMQRTFSSQSRQNVTQALPELHLGNAIIKPSRYVQNNQFSPLKKYPLV
                     EQFRYPLWQAKPVEPQQGVKLEGASSNFISPQPGNIYIPLGQQEPGLYLVEAMVGGYR
                     ATTVVFVSDTVALSKVSGNELLVWTAGKKQGEAKPGSEILWTDGLGVMTRGVTDDSGT
                     LQLQHISPERSYILGKDAEGGVFVSENFFYESEIYNTRLYIFTDRPLYRAGDRVDVKV
                     MGREFHDPLHSSPIVSAPAKLSVLDANGSLLQTVNVTLDARNGGQGSFRLPENAVAGG
                     YELRLAYRNQVYSSSFRVANYIKPHFEIGLALDKKEFKTGEAVSGKLQLLYPDGEPVK
                     DARVQLSLRAQQLSMVGNDLRYAGRFPVSLEGSETVSDASGHVALNLPAADKPSRYLL
                     TVSASDGAAYRVTTTKEILIERGLAHYSLSTAAQYSNSGESVVFRYAALESSKQVPVT
                     YEWLRLEDRTSHSGELPSGGKSFTVNFAKPGNYNLTLRDKDGLILAGLSHAVSGKGST
                     AHTGTVDIVADKTLYQPGETAKMLITFPEPIDEALLTLERDRVEQQSLLSHPANWLTL
                     QRLNDTQYEARVPVSNSFAPNITFSVLYTRNGQYSFQNAGIKVAVPQLDIRVKTDKTH
                     YQPGELVNVELTSSLKGKPVSAQLTVGVVDEMIYALQPEIAPNIGKFFYPLGRNNVRT
                     SSSLSFISYDQALSSEPVAPGATNRSERRVKMLERPRREEVDTAAWMPSLTTDKQGKA
                     YFTFLMPDSLTRWRITARGMNGDGLVGQGRAYLRSEKNLYMKWSMPTVYRVGDKPAAG
                     LFIFSQQDNEPVALVTKFAGAEMRQTLTLHKGANYISLTQNIQQSGLLSAELQQNGQV
                     QDSISTKLSFVDNSWPVEQQKNVMLGGGENALMLPEQASNIRLQSSETPQEIFRNNLD
                     ALVDGPWGGVINTGSRLIPLSLAWRSLADHQSAAANDIRQMIQDNRLRLMQLAGPGAH
                     FTWWGEDGNGDAFLTAWAWYADWQASQALGVTQQPEYWQHMLDSYAEQADNMPLLHRA
                     LVLAWAQEMNLPCKTLLKGLDEAIARRGTKDEDFSEEDIRDINDSLILDTPESPLADA
                     VANVLTMTLLKKAQLKSTVMPQVQQYAWDKAANSNQPLAHTVVLLNSGGDATQTAAIL
                     SGLTAEQSTIERALAMNWLAKYMATMPPVVLPAPAGAWAKHKLTGGGEDWRWVGQGVP
                     DILSFGDELSPQNVQVRWREPAKMAQQSNIPVTVERQLYRLIPGEEEMSFILQPVTSN
                     EIDSDALYLDEITLTSEQDAVLRYGQVEVPLPPGADVERTTWGISVNKPNAAKQQGQL
                     LEKARNEMGELAYMVPVKELTGTVTFRHLLRFSQKGQFVLPPARYVRSYAPAQQSVAA
                     GSEWTGMQVK"
     gene            4662..6311
                     /gene="yfaQ"
                     /locus_tag="ACHOSC_RS13605"
                     /old_locus_tag="pgaptmp_002721"
     CDS             4662..6311
                     /gene="yfaQ"
                     /locus_tag="ACHOSC_RS13605"
                     /old_locus_tag="pgaptmp_002721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfaQ family protein"
                     /protein_id="HGU4548634.1"
                     /translation="MNWRRIVWLLALVTLPTLAEETPLQLVLRGAQHDQLYQLSSSGV
                     TKVSALPDSLTTPLGSLWKQYVYAWLEDTHQPEQPYQCRGNSPEEVYCCQAGESITRD
                     TALVRSCGLYFAPQRLHIGADVWGQYWQQRQAPAWLASLTTLKPETSVTVKSLLDSLA
                     TLPAQNKAQEVLLDVVLDEAKIGVASMLGSRVRVKTWSWFADDKQEIRQGGFAGWLTD
                     GTPLWVTGSGTSKTVLTRYATVLNRVLPVPTQVASGQCVEVELFARYPLKKITAEKST
                     TAVKPGVLNGRYRVTFTNGNHITFVSHGETTLLSEKGKLKLQSHLDREEYVARVLDRE
                     AKSTPPEAAKAMTVAIRTFLQQNANREGDCLTIPDSSATQRVSASPATTGARTMTAWT
                     QDLIYAGDPVHYHGSRATEGTLSWRQATAQAGQGERYDQILAFAYPDNSLSRWGAPRS
                     TCQLLPKAKAWLAKKMPQWRRILQGETGYNEPDVFAVCRLVSGFPYTDRQQKRLFIRN
                     FFTLQDRLDLTHEYLHLAFDGYPTGLDENYIETLTRQLLMD"
     gene            6316..7092
                     /gene="yfaP"
                     /locus_tag="ACHOSC_RS13610"
                     /old_locus_tag="pgaptmp_002722"
     CDS             6316..7092
                     /gene="yfaP"
                     /locus_tag="ACHOSC_RS13610"
                     /old_locus_tag="pgaptmp_002722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfaP family protein"
                     /protein_id="HGU4548635.1"
                     /translation="MRKIFLPLLLVALSPVAHSEGVQEVEIDAPLSGWHPAEGEDASF
                     SQSINYPASSVNMADDQNISAQIRGKIKNYAAAGKVQQGRLVVNGASMPQRIESDGSF
                     ARPYIFTEGSNSVQVISPDGQSRQKMQFYSTPGTGTIRARLRLVLSWDTDNTDLDLHV
                     VTPDGEHAWYGNTVLKNSGALDMDVTTGYGPEIFAMPAPVHGRYQVYINYYGGRSETE
                     LTTAQLTLITDEGSVNEKQETFIVPMRNAGELTLVKSFDW"
     gene            7367..10216
                     /gene="rcsC"
                     /locus_tag="ACHOSC_RS13615"
                     /old_locus_tag="pgaptmp_002723"
     CDS             7367..10216
                     /gene="rcsC"
                     /locus_tag="ACHOSC_RS13615"
                     /old_locus_tag="pgaptmp_002723"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416722.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0018106 - peptidyl-histidine
                     phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     RcsC"
                     /protein_id="HGU4548636.1"
                     /translation="MKYLASFRTTLKASRYMFRALALVLWLLIAFSSVFYIVNALHQR
                     ESEIRQEFNLSSDQAQRFIQRTSDVMKELKYIAENRLSAENGVLSPRGRETQADVPAF
                     EPLFADSDCSAMSNTWRGSLESLAWFMRYWRDNFSAAYDLNRVFLIGSDNLCMANFGL
                     RDMPVERDTALKALHERINKYRNAPQDDSGSNLYWISEGPRPGVGYFYALTPVYLANR
                     LQALLGVEQTIRMENFFLPGTLPMGVTILDENGHTLISLTGPESKIKGDPRWMQERSW
                     FGYTEGFRELVLKKNLPPSSLSIVYSVPVDKVLERIRMLILNAILLNVLAGAALFTLA
                     RMYERRIFIPAESDALRLEEHEQFNRKIVASAPVGICILRTADGVNILSNELAHTYLN
                     MLTHEDRQRLTQIICGQQVNFVDVLTSNNTNLQISFVHSRYRNENVAICVLVDVSSRV
                     KMEESLQEMAQAAEQASQSKSMFLATVSHELRTPLYGIIGNLDLLQTKELPKGVDRLV
                     TAMNNSSSLLLKIISDILDFSKIESEQLKIEPREFSPREVMNHITANYLPLVVRKQLG
                     LYCFIEPDVPVALNGDPMRLQQVISNLLSNAIKFTDTGCIVLHVRADGDYLSIRVRDT
                     GVGIPAKEVVRLFDPFFQVGTGVQRNFQGTGLGLAICEKLISMMDGDISVDSEPGMGS
                     QFTVRIPLYGAQYPQKKGVEGLSGKRCWLAVRNASLCQFLETSLQRSGIVVTTYEGQE
                     PTPEDVLITDEVVSKKWQGRAVVTFCRRHIGIPLEKAPGEWVHSVAAPHELPALLARI
                     YLIEMESDDPANALPSTDKAVSDNDDMMILVVDDHPINRRLLADQLGSLGYQCKTAND
                     GVDALNVLSKNHIDIVLSDVNMPNMDGYRLTQRIRQLGLTLPVIGVTANALAEEKQRC
                     LESGMDSCLSKPVTLDVIKQTLTVYAERVRKSRES"
     gene            complement(10302..10952)
                     /gene="rcsB"
                     /locus_tag="ACHOSC_RS13620"
                     /old_locus_tag="pgaptmp_002724"
     CDS             complement(10302..10952)
                     /gene="rcsB"
                     /locus_tag="ACHOSC_RS13620"
                     /old_locus_tag="pgaptmp_002724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor RcsB"
                     /protein_id="HGU4548637.1"
                     /translation="MNNMNVIIADDHPIVLFGIRKSLEQIEWVNVVGEFEDSTALINN
                     LPKLDAHVLITDLSMPGDKYGDGITLIKYIKRHFPSLSIIVLTMNNNPAILSAVLDLD
                     IEGIVLKQGAPTDLPKALAALQKGKKFTPESVSRLLEKISAGGYGDKRLSPKESEVLR
                     LFAEGFLVTEIAKKLNRSIKTISSQKKSAMMKLGVENDIALLNYLSSVTLSPADKD"
     gene            complement(10969..13641)
                     /gene="rcsD"
                     /locus_tag="ACHOSC_RS13625"
                     /old_locus_tag="pgaptmp_002725"
     CDS             complement(10969..13641)
                     /gene="rcsD"
                     /locus_tag="ACHOSC_RS13625"
                     /old_locus_tag="pgaptmp_002725"
                     /EC_number="2.7.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0009927 - histidine phosphotransfer kinase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotransferase RcsD"
                     /protein_id="HGU4548638.1"
                     /translation="MRQKETTATTRFSLLPGSITRFFLLLIIVLLVTMGVMVQSAVNA
                     WLKDKSYQIVDITHAIQKRVDTWRYVTWQIYDNIAATTSPSSGEGLQETRLKQDVYYL
                     EKPRRKTEALIFGSHDNSTLEMTQRMSTYLDTLWGAENVPWSMYYLNGQDNSLVLIST
                     LPLKDLTSGFKESTVSDIVDSRRAEMLQQANALDERESFSNMRRLAWQNGHYFTLRTT
                     FNQPGHLATVVAFDLPINDLIPPGMPLDSFRLEPDATATGNNDNEKEGTDSVSIHFNS
                     TKIEISSALNSTDMRLVWQVPYGTLLLDTLQNILLPLLLNIGLLALALFGYTTFRHFS
                     SRSTESLPSTAVNNELRILRAINEEIVSLLPLGLLVHDQESNRTVISNKIADHLLPHL
                     NLQNITTMAEQHQGIIQATINNELYEIRMFRSQVAPRTQIFIIRDQDREVLVNKKLKQ
                     AQRLYEKNQQGRMTFMKNIGDALKEPAQSLAESAAKLNAPESKQLANQADVLVRLVDE
                     IQLANMLADDSWKSETVLFSVQDLIDEVVPSVLPAIKRKGLQLLINNHLKAHDMRRGD
                     RDALRRILLLLMQYAVTSTQLGKITLEVDQDESSEDRLTFRILDTGEGVSIHEMDNLH
                     FPFINQTQNDRYGKADPLAFWLSDQLARKLGGHLNIKTRDGLGTRYSVHIKMLAADPE
                     VEEEEERLLDDVCVMVDVTSAEIRNIVTRQLENWGATCITPDERLISQDYDIFLTDNP
                     SNLTASGLLLSDDESGVREIGPGQLCVNFNMSNAMQEAVLQLIEVQLAQEEVTESPLG
                     GDENAQLHASGYYALFVDTVPDDVKRLYTEAATSDFAALAQTAHRLKGVFAMLNLVPG
                     KQLCETLEHLIREKDVPGIEKYISDIDSYVKSLL"
BASE COUNT         3390 a         3438 c         3769 g         3428 t
ORIGIN      
        1 atgattttta atgtattttt gtaatttact ctccttctga catggaagga cacccgatgg
       61 atacccaacg attccaatcc caatttcatt ggcatttatc gtttaaattt tctggcgcga
      121 tagcagcgtg cttgtccctg tctcttgtgg gaacagggct tgctaatgct gatgattcgc
      181 ttccttccag taactatgcg ccgcccgccg ggggaacatt ctttttgctt gctgacagca
      241 gttttagcag cagtgaagag gcgaaagtgc gactggaagc gccggggcgt gattatcggc
      301 gctatcagat ggaagagtac ggcggcgtgg acgttcgcct gtatcgtatt cctgacccga
      361 tggcattttt gcgccagcag aaaaacctgc atcgcattgt ggtgcaaccg caatatctgg
      421 gcgacgggct gaacaatacg ctgacctggc tgtgggataa ctggtacggc aaatctcgcc
      481 gcgtaatgca gcgtactttc tcttctcagt cacggcagaa tgtgactcag gcattacccg
      541 aattacatct cggcaatgcc attattaaac cttcccgtta tgtacagaac aaccagtttt
      601 ccccgctgaa aaaatatccc ttggtggaac agttccgtta tccactatgg caggctaaac
      661 cggtcgagcc gcagcaaggg gtaaaactgg aaggcgcatc cagcaatttc atctcgccgc
      721 agccgggtaa catttatatt cctctcggcc aacaagagcc gggactgtac ctcgtcgagg
      781 cgatggttgg tgggtatcgg gcgacgacgg tggtgtttgt ttccgatacc gtggcgctta
      841 gcaaagtgtc aggcaacgag cttctggtgt ggaccgcggg taaaaaacag ggtgaagcga
      901 agcccggctc agagatcttg tggactgacg gtcttggcgt gatgacccgc ggtgtgaccg
      961 atgacagcgg taccttgcag ttacaacata tatcgccaga acgttcatac attctgggta
     1021 aggatgctga aggcggtgtt tttgtctccg agaacttctt ctacgaaagc gaaatctaca
     1081 acacccgctt gtatattttt accgatcgcc cgctatatcg cgcaggcgat cgtgtcgatg
     1141 ttaaagtgat gggccgcgag ttccacgatc cgttgcattc atcccccatc gtcagcgccc
     1201 cggcgaagct ttcggtgctg gacgctaacg gcagtctgtt gcaaaccgtc aatgtcacgc
     1261 tggatgcgcg caatggcggg cagggaagtt tccgcctgcc agaaaatgcc gtagccggag
     1321 gttatgagtt acgtcttgct taccgcaatc aggtctatag cagcagtttt cgcgtggcaa
     1381 actacatcaa gccacatttc gagattggtt tagctctcga caaaaaagag ttcaaaactg
     1441 gcgaagcggt cagcggcaaa ctgcaactgc tttacccgga tggtgagccg gtaaaagatg
     1501 cccgcgtgca gttaagtttg cgcgctcagc aattatcaat ggtcggtaac gatttgcgtt
     1561 atgccggacg ttttcccgtg tcgctggaag gcagcgaaac ggtgtccgac gccagcggtc
     1621 atgtggcgtt aaatctcccc gccgccgata aaccgagccg ctatttgtta accgtctccg
     1681 ccagtgacgg cgcggcgtat cgcgtcacca ccaccaaaga gatcctcatt gaacgcggcc
     1741 tggcgcatta ctctttaagt accgccgcac aatacagtaa tagcggcgag tcggttgtgt
     1801 tccgttatgc cgcgctggaa tcttcaaaac aggttcctgt tacgtatgaa tggttgcgtc
     1861 tcgaagaccg cacgagccat agcggagagc taccgtcagg cggcaaatcc tttaccgtca
     1921 atttcgctaa acctggcaac tacaatctga cattacgcga taaagacggc ttaattctcg
     1981 ctgggttaag tcatgccgtc agcggtaagg gcagcacggc gcatactggt acggtagata
     2041 tcgtggcgga taaaacgctg taccagccag gcgaaactgc gaagatgctg attacctttc
     2101 cggagccaat tgatgaagca ttattgacgc tggaacgcga tcgcgtggaa cagcagtcgc
     2161 tgctttcgca tccggcaaac tggctgacgt tacaacgttt aaacgatacc cagtatgaag
     2221 cccgggttcc agtgagcaat tcctttgcgc ctaacatcac tttttcggtg ctgtataccc
     2281 gtaatggtca gtacagtttt cagaacgccg ggatcaaagt tgccgttcct cagcttgata
     2341 tccgggtgaa aacggacaaa acccattacc agcctggtga actggtcaat gtcgaattaa
     2401 cctcgtcgct gaaaggtaaa cctgtttctg cgcagctaac ggtaggcgtg gtcgatgaaa
     2461 tgatctacgc gctgcaacca gaaatcgcgc cgaatatcgg caaatttttc tatccgctgg
     2521 ggcgtaacaa tgtgcgtacc agctccagtc tgtcgtttat cagctacgac caggcgctct
     2581 ccagcgagcc ggttgcgcct ggcgcaacta accgcagcga gcggcgagta aaaatgcttg
     2641 aacgtccacg gcgtgaagag gtggataccg cggcatggat gccgtcactc acaaccgata
     2701 aacaaggcaa agcgtatttc acgttcctga tgcctgattc gttaacccgc tggcgtatca
     2761 ccgcgcgtgg gatgaacggc gacgggctgg tcgggcaggg gcgtgcttat ctgcgttcgg
     2821 aaaaaaatct ctacatgaag tggagtatgc caacggtgta tcgcgtgggc gacaaaccgg
     2881 cggcaggact gtttatcttc agtcagcagg ataacgaacc ggtggcgctg gtgactaaat
     2941 ttgcaggcgc tgagatgcgc cagacgctga cgctgcacaa aggggcgaat tatatttcgc
     3001 tgacgcagaa cattcagcaa tctggcttgt taagtgcaga actgcaacaa aatgggcaag
     3061 tgcaggacag cattagcaca aaactgtctt ttgtggacaa cagctggccc gttgaacagc
     3121 agaaaaatgt catgctcggc ggtggcgaga acgcgctgat gttgcccgag caggcgagta
     3181 atatccggct acaaagtagt gaaacgccgc aggagatttt ccgcaacaat cttgatgcgt
     3241 tagtcgatgg accatggggt ggcgtgatca acaccggtag ccgtctgatc ccgctcagtc
     3301 tcgcctggcg ttcgcttgcc gatcatcaaa gtgccgccgc taacgacatt cgtcagatga
     3361 ttcaggataa ccgtctgcgg ctgatgcaac tggcggggcc cggagcgcac tttacctggt
     3421 ggggtgaaga tggcaatggt gacgccttcc ttacggcatg ggcatggtac gccgactggc
     3481 aggccagcca ggcgctcggc gtaacgcaac aaccggaata ctggcagcat atgctcgaca
     3541 gttatgccga gcaggcggat aacatgccgt tattgcatcg ggcgctggtg ctggcgtggg
     3601 cacaggagat gaatctgccg tgcaaaacgt tgttgaaagg gttggatgaa gctatcgccc
     3661 ggcgcggaac taaagatgaa gatttctctg aggaagacat ccgcgatatc aatgacagcc
     3721 tgatcctcga tacaccggaa tctccactgg cagatgcggt ggcaaacgtc ttaaccatga
     3781 cgttgctgaa aaaagcgcag ttgaagtcca cggtgatgcc acaggttcag caatatgcgt
     3841 gggataaagc ggcaaacagc aatcagccgc tggcgcacac ggttgtgctg ctcaatagcg
     3901 gtggtgacgc tacccagacg gccgctattt taagtggttt gaccgctgag caatccacta
     3961 ttgagcgcgc gctggccatg aactggctgg cgaaatatat ggcgacaatg cctccagttg
     4021 ttttgcctgc gcctgcgggc gcatgggcta aacataagtt aactggaggg ggcgaagact
     4081 ggcgttgggt tggtcagggc gtgccggaca ttctctcttt tggtgacgaa ttatcgccgc
     4141 aaaatgtgca ggtccgctgg cgtgagccgg caaaaatggc tcaacaaagt aacattccgg
     4201 tgaccgttga acgccagttg tatcggctta tccctggtga agaagagatg agctttattc
     4261 tgcaaccggt gaccagcaat gagattgaca gcgatgcgct gtatctcgat gaaattacgc
     4321 ttaccagcga gcaggatgca gttctgcgct atggtcaggt ggaagtaccg ctcccgccgg
     4381 gagccgacgt tgagcgcaca acatggggca tttcagtcaa taaacccaac gccgcgaaac
     4441 agcaggggca attgctggaa aaagcgcgta atgaaatggg cgaactggcc tatatggtgc
     4501 cggtgaaaga actgacggga acggtcactt tccgccattt gctgcgcttc tcgcaaaaag
     4561 ggcaattcgt tctgcctcct gctcgttatg tgcgttccta tgcacctgcg cagcaaagtg
     4621 ttgcggcagg gagtgaatgg accgggatgc aggtgaaata agtgaactgg cgcagaatcg
     4681 tctggctatt ggcgttggtt actctgccaa cgcttgcgga ggagacgccg ttacaactgg
     4741 tgctgcgcgg tgcgcagcac gatcaactgt atcagctgtc gtcatcaggc gtgaccaaag
     4801 tctcagcatt acctgactcg ctcaccacgc cgttagggag tttgtggaaa cagtatgttt
     4861 acgcctggct ggaggatacc catcaaccgg agcagcccta ccagtgtcgc ggaaactccc
     4921 cggaagaggt ttattgttgt caggctgggg agagcataac gcgcgatacc gcgctggtac
     4981 gttcctgtgg gctctatttt gcgccgcagc ggttgcatat tggcgcagat gtgtgggggc
     5041 aatactggca acagcgtcag gcacctgcgt ggttagcgtc tcttacaacg ctgaagccag
     5101 aaacctcggt aacggttaaa tcgctgctcg attcattagc gacgttacct gcgcaaaaca
     5161 aagctcagga agtcttactg gatgtggtgc tggacgaggc gaaaatcggt gtcgcatcca
     5221 tgctcggtag tcgggtacgg gtgaaaacct ggagctggtt tgccgatgat aaacaggaaa
     5281 tccgccaggg tggttttgct ggctggttaa ccgatggtac accgctgtgg gttactggca
     5341 gtggcaccag caaaacggta ctaacccgtt atgcgacggt gcttaatcgg gttttgccag
     5401 tgccgacgca ggttgccagt gggcaatgtg tagaggtgga gctgtttgcc cgctatccgc
     5461 tgaagaaaat cacagcggaa aagagtacga cggcagtaaa acccggcgta cttaatgggc
     5521 gctatcgtgt gacgtttact aatggtaacc acattacgtt tgtcagccac ggtgagacga
     5581 ccttactgag cgaaaagggc aaactgaaat tgcagtcgca tctcgatcgt gaagagtacg
     5641 ttgccagagt tctggatcgc gaggcgaaaa gtacgccccc tgaggcagcg aaagccatga
     5701 ccgtggcgat tcgcactttt ttacagcaaa acgccaatcg ggaaggcgat tgcctgacca
     5761 tccccgacag cagcgccacg cagcgtgttt ctgcttcgcc agcgaccacg ggggcgcgaa
     5821 cgatgaccgc ctggacgcag gatctcatct atgcaggcga tccggttcat tatcatggca
     5881 gtcgagctac cgaagggaca ctttcctggc ggcaagccac ggcccaggcg gggcagggag
     5941 agcgttacga ccagatactc gcgtttgctt atcccgacaa cagccttagc cgctggggag
     6001 cgccacgctc aacctgccag ttattaccca aagcgaaagc ctggctggcg aaaaaaatgc
     6061 cgcagtggcg gcgtatatta caaggcgaga cggggtacaa cgaaccagac gtgtttgcgg
     6121 tctgtcgttt agtctcaggt ttcccctata ccgatcggca gcagaaacgg ctgtttattc
     6181 gtaacttctt tacgcttcag gatcggctgg atttaaccca tgagtatctg cacctggcct
     6241 ttgatggtta tcccaccggg cttgatgaga actatatcga aacgctgacc cgacaattat
     6301 taatggacta atgctatgcg aaagattttt cttccgttgt tactggtggc actttcgcct
     6361 gtcgctcaca gtgaaggtgt gcaggaagtc gagattgacg caccgctctc tggctggcat
     6421 ccggcagagg gcgaagatgc cagtttttca caatcgatca attacccggc gtcgtccgtc
     6481 aacatggcag atgatcaaaa tatctcagcg cagatccgcg gcaaaataaa gaattatgct
     6541 gcggcgggta aagttcagca gggtcggctg gtggtcaacg gtgccagtat gccgcagaga
     6601 attgaatccg atggttcatt tgcacgccct tatattttca ctgaaggcag caacagcgtg
     6661 caggtcatca gcccggatgg gcaaagccgg caaaaaatgc agttttactc aacgccgggt
     6721 acaggaacga ttcgtgcacg tttacggctg gttctctcgt gggatacgga caataccgat
     6781 ctcgatctcc atgttgtcac gcccgatggc gaacacgcct ggtacggtaa caccgtgctg
     6841 aaaaacagtg gtgcactgga tatggatgtc acgacggggt acggacccga gattttcgcc
     6901 atgccagcac cagttcacgg gcgctatcag gtgtatatca actactatgg tgggcgcagc
     6961 gaaacggaat tgacgaccgc ccaactgacg ctgatcaccg atgaaggatc ggtcaatgag
     7021 aaacaggaaa catttattgt accgatgcgt aatgctggcg agctgacgct ggtgaaaagt
     7081 tttgactggt gatccatccc ttcacacaac ctggctctct cttttttcag ataagactgc
     7141 tgggcgatct gccgacgcca ggatataacc attaacaagc gtcggcagat tggtcatttc
     7201 ttatttttcg tactgagaac tgtccatcta ctccggcttt cctggttaag gcaatggttt
     7261 ctaagcgata cgcattattg caatggtgac agaacattgc ggtaacacgc ttttaccgct
     7321 accttaacca cactccatcg gtcacctgag gcggagcttc gcccctttga aataccttgc
     7381 ttcttttcgt acaaccctga aagcctcgcg ctacatgttc agagcattgg cgttagtgct
     7441 ctggctgttg attgcttttt catccgtttt ttacatcgtt aatgcgttac atcagcgaga
     7501 atcggaaatt cgtcaggaat ttaatctgag ttccgatcag gctcagcgct ttattcaacg
     7561 cacctctgat gtgatgaaag agctgaagta catcgccgaa aatcgcttat cggcagaaaa
     7621 cggtgtgctt tccccgcgtg gacgagaaac gcaggcggat gtgcctgcgt ttgaaccgct
     7681 gtttgccgac tccgattgtt ccgcaatgag taacacctgg cgaggttctc tggagtcatt
     7741 ggcgtggttt atgcgctact ggcgcgataa tttttctgcg gcttacgatc tcaaccgggt
     7801 atttttaatc ggcagtgata acctctgcat ggccaatttc ggtctgcgtg atatgccagt
     7861 ggaacgcgat accgcgttga aagcgttgca tgaacgcatc aataaatatc gaaatgcacc
     7921 acaagatgat agcggcagta acctctactg gatcagcgaa ggtccgcgcc ctggcgtcgg
     7981 gtatttttac gcgttgacgc cagtttatct ggcgaaccgg ttgcaggcgc ttttgggtgt
     8041 cgagcagacc atccggatgg aaaacttttt cttaccgggt acgttgccga tgggggttac
     8101 cattcttgat gaaaatggtc ataccctgat ttcgcttacc ggaccagaaa gtaaaattaa
     8161 gggcgatcct cgctggatgc aggaacgctc ctggtttggc tatacggaag ggttccggga
     8221 gctggtgctg aagaaaaatc tgccaccctc atcgctaagc atcgtgtatt cggtgccggt
     8281 tgataaggtg ctggaacgca ttcgcatgtt gatccttaac gcaattttgc tgaatgtgct
     8341 tgccggagct gcattgttta ctctcgcacg gatgtacgag cgacgtattt tcattccggc
     8401 ggaaagcgac gccctgcgac tggaagaaca tgagcagttc aatcgcaaga ttgtcgcctc
     8461 cgcgccagtg ggtatctgca ttttgcgtac cgctgatggc gtcaatattt taagtaacga
     8521 actggcgcat acctatctca atatgcttac gcatgaggac cgccaacgac tgacgcaaat
     8581 tatctgtggg cagcaggtca attttgttga tgtcctgacc agcaacaata ccaatctgca
     8641 aatcagcttc gtccattcgc gctatcgtaa tgaaaacgtg gccatttgtg tgctggtgga
     8701 tgtttcttcg cgcgtgaaga tggaagagtc gttgcaggag atggcacaag cagcggaaca
     8761 ggcgagccag tcaaaatcga tgttccttgc caccgtcagt catgagctgc gaacgccgct
     8821 gtatggcatt atcggtaacc tggatctgtt gcaaaccaaa gagttaccga aaggcgtcga
     8881 tcggctggtg acggcaatga acaactcttc cagcctgttg ttgaaaatta tcagcgatat
     8941 tctcgatttc tcgaagattg aatcggaaca gttgaagatc gaaccgcgtg agttttcacc
     9001 gcgtgaagtg atgaaccaca tcaccgccaa ctatttaccg ctggtggtac gcaagcagtt
     9061 aggcttgtac tgctttattg aaccggatgt gccagtggcc ttaaatggcg acccgatgcg
     9121 tttacagcag gtcatctcca acctgctgag taacgccata aaattcaccg ataccggctg
     9181 tatagttttg catgttcgcg ctgatggcga ttatctctct atccgtgttc gcgataccgg
     9241 cgtgggcatt ccggcgaaag aagtggtgcg cttgtttgat cccttcttcc aggtcggaac
     9301 gggcgtacag cgtaacttcc aggggaccgg tctgggtctg gcgatttgtg aaaaactgat
     9361 cagcatgatg gacggcgata tctcggtaga ttcagaaccg ggaatgggca gccagtttac
     9421 cgtgcgtatt ccgttgtacg gcgctcagta cccgcagaaa aaaggcgtgg aagggttgag
     9481 tggtaaacgc tgctggctgg cggtccgcaa tgcgtcgctc tgtcagttcc tggaaaccag
     9541 tttgcagcgc agcggcatcg tcgttacaac atacgaaggg caggaaccga ctcccgaaga
     9601 tgtgttaatc actgacgagg tagtgagtaa aaaatggcag ggtagagcgg tagtgacctt
     9661 ctgtcgtcgc catattggta ttccgctgga gaaagcgcca ggggagtggg tacacagtgt
     9721 ggctgctccg catgagctac cggcattgtt ggcgcgtatt tatttgatcg agatggagag
     9781 cgacgatcct gctaacgctc tgccgtcgac ggacaaagcg gtcagcgata acgacgatat
     9841 gatgattctg gtcgtggatg atcatccgat taaccgacgt ctgctggcag atcagttggg
     9901 atcgttgggc tatcaatgta aaaccgcgaa tgatggcgtc gatgcgctta atgtacttag
     9961 caagaatcat attgatatcg tgcttagcga cgtcaacatg ccaaatatgg atggttaccg
    10021 cttgacgcaa cgcattcgtc agttgggact gacgttgccg gtaatcggag taactgctaa
    10081 tgcgttggct gaagagaagc agcggtgtct ggagtccggt atggacagct gcctgtcgaa
    10141 gccggtaacg ctggatgtga taaaacagac gctgacggta tatgccgaga gggtcaggaa
    10201 atcgcgggaa tcgtaggacg gataaggcgt ttacgccgca tccggcattc agtgcaaatg
    10261 ccagataaga cactaacgcg tcttatctgg cctacaggtg attagtcttt atctgccgga
    10321 cttaacgtca cagaagagag ataattcagc agggcgatat cgttctcgac acccagcttc
    10381 atcatcgcag atttcttctg gctactgatg gttttaatac tgcggttcag ctttttagcg
    10441 atctcggtca ccaggaagcc ttccgcaaac aggcgcagaa cttcactctc ttttggcgag
    10501 agacgcttgt caccgtaacc accagcactg attttttcca acaggcgaga aacgctttcc
    10561 ggggtaaatt tcttcccttt ctgcagcgcg gcgagagctt tcggcagatc ggtcggtgca
    10621 ccttgtttca gcacgatccc ttcgatatcc agatccaata ccgcactaag aatcgccggg
    10681 ttgttgttca tggtcagaac aataatcgac aggcttggga aatggcgctt gatgtacttg
    10741 attaaggtaa tgccatcgcc gtacttatcg ccaggcatgg agagatcggt aatcaacaca
    10801 tgcgcatcca gtttcggcag gttgttgatc agtgctgtag agtcttcaaa ttcgccgaca
    10861 acattcaccc actcaatttg ctcaagtgat ttgcgaatac cgaacaagac tatcggatgg
    10921 tcatcggcaa taattacgtt catattgttc atgtaatagg ctaccttgct acagcaagct
    10981 cttgacataa ctgtcaatgt cgctgatgta tttttctatt cctggaacat ccttctcacg
    11041 aatcagatgt tccagcgttt cacataactg cttgccgggt accagattta gcatggcaaa
    11101 tacgccttta agacgatgag ccgtttgggc taacgcagca aagtcactgg ttgctgcttc
    11161 agtatacagc ctcttaacat catccggtac tgtgtctaca aagagcgcat aatagccgct
    11221 ggcatggagt tgcgcatttt catctccgcc cagaggcgat tctgtcacct cttcctgcgc
    11281 cagttgcact tcaattaatt gtaagaccgc ttcctgcata gcgttgctca tattgaagtt
    11341 gacgcacaat tgaccaggcc caatttcccg tacgccagac tcatcatcgc ttaaaagcaa
    11401 gccagaggca gtaagattag acggattatc cgttaaaaag atatcataat cttgactaat
    11461 taatctttca tcgggtgtga tacaggttgc accccaattt tctaactggc gagtgacaat
    11521 attccgaatt tctgccgaag taacatccac cattacgcag acatcatcca gtaaacgctc
    11581 ttcttcctct tcaacttccg ggtcagcggc gagcattttg atatgcacag agtagcgtgt
    11641 accaagccca tcccgcgttt tgatgtttaa atgaccgccc agtttacgcg ccagttgatc
    11701 gctcagccag aatgccagcg ggtccgcctt gccatagcga tcgttttggg tctggttgat
    11761 aaacgggaag tgcaaattat ccatttcatg aatacttacg ccttctccgg tgtccagaat
    11821 gcggaatgtc aggcggtctt cagaggactc atcctgatca acctcaaggg tgatttttcc
    11881 caattgcgtt gaggtcacgg catattgcat cagtagcagc aaaatacgtc gtaaggcatc
    11941 gcgatcgccg cggcgcatat cgtgtgcttt cagatgattg ttaatcagca gttgcagacc
    12001 tttacgcttg atggcaggca acactgaagg cacaacttca tcaattaaat cctgcacgga
    12061 gaacagcacc gtctcacttt tccagctatc atccgcaagc atgttcgcta actgtatttc
    12121 atcaaccagc cgcaccagca catctgcctg attcgccagt tgtttgcttt ccggggcgtt
    12181 gagtttagcc gcgctctccg ccagggactg tgcaggttct ttcagcgcat cgccaatgtt
    12241 tttcataaag gtcatccggc cctgctggtt tttctcatac agacgctgcg cctgcttgag
    12301 tttcttgttt accagcactt cgcgatcctg atcgcgaata atgaaaattt gtgtgcgcgg
    12361 cgcgacctgg ctgcggaaca tgcggatctc atacagctcg ttattgatcg tcgcctgaat
    12421 aatcccctga tgctgttccg ccatggtggt gatgttttgc agattcaaat gcggcagcaa
    12481 atgatcggca attttgttac ttatgacagt gcggttcgat tcctgatcgt gaaccagcag
    12541 gccgagcggc agcagtgaga ctatctcttc attaattgcc cgtaaaatgc gcaattcgtt
    12601 attgaccgcc gtgctgggta gactttctgt actgcggctg gagaaatggc ggaatgtggt
    12661 atagccaaat aacgccagcg ccagcaaacc gatgttcagc agcagtggca gcagaatgtt
    12721 ttgcaacgta tccagcaata aggtgccata aggaacctgc cagaccaggc gcatatcggt
    12781 agagttgagt gccgaggaga tttcaatctt cgtactgtta aagtggatac tgacgctatc
    12841 cgtcccttct ttctcattat cattgtttcc cgttgccgtc gcgtctggct caaggcggaa
    12901 actgtccagc ggcatacccg gtgggatcaa atcattaatc ggcagatcaa aagccacgac
    12961 cgttgccaga tgtcctggct ggttaaaggt agtacgcaag gtaaagtaat gaccgttctg
    13021 ccaggccagg cggcgcatgt tagaaaagct ttcgcgttca tcgagggcgt tggcctgttg
    13081 caacatctct gcacgacgtg aatcaacaat gtcgctgacg gtcgattctt taaatccgga
    13141 ggtgagatct ttaaggggta gggttgagat cagcaccaga ctgttatcct gaccattcag
    13201 gtaatacatc gaccacggta cattttctgc gccccacaat gtatccagat aagtggacat
    13261 ccgctgagtc atctcaagcg ttgagttgtc gtgagagcca aagattaacg cttccgtttt
    13321 gcggcgtggt ttctccagat agtagacatc ctgtttcagg cgcgtctctt gtaaaccttc
    13381 gccggaggag ggggaggtcg tcgcggcaat gttgtcgtag atctgccagg tcacgtaacg
    13441 ccaggtatcg acgcgctttt ggatagcgtg ggtaatgtcg acaatctggt aacttttatc
    13501 tttcagccat gcgttaacgg cgctttgtac cattacaccc atcgtcacca gtaacacaat
    13561 gatcaacagt aaaaagaagc gggtaatgct ccccggtagg agtgaaaagc gggtcgtggc
    13621 cgttgtctct ttctgacgca ttcgtgttta tgacctgtta aaacttcgcg aagtgatagg
    13681 gcagtataaa gggtacagtg tgaaattcca gactcttacc tgattgatga ttctctattc
    13741 tcccgtgata tttatcatcc tgcattgggg gaaatagatg tacaagcgcc attttgctgt
    13801 acaaataacc tacaaaaagc ccaacgaaaa taattgcaaa taaacaaaga ttgctggaaa
    13861 ttatgctgat gttaattatt tgtgaaatag ttaacaagcg ttatagtttt tctgtggtag
    13921 caaagaataa tgaaaagtgt gtaaagaagg gtaaaaaaaa ccgaatgcga ggcatccggt
    13981 tgaaataggg gtaaacagac attcagaaat gaatgacggt aataa
//
LOCUS       DAZKHF020000115        13884 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2201S31241
            SAMN43267800-rid25106413.denovo.116, whole genome shotgun sequence.
ACCESSION   DAZKHF020000115 DAZKHF020000000
VERSION     DAZKHF020000115.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267800
            Sequence Read Archive: SRR30307263
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 13884)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13884)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 13884)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350581.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350581.2
            Genome Coverage       :: 151x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:21:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,652
            CDSs (total)                      :: 5,557
            Genes (coding)                    :: 5,320
            CDSs (with protein)               :: 5,320
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 237
            CDSs (without protein)            :: 237
            Pseudo Genes (ambiguous residues) :: 0 of 237
            Pseudo Genes (frameshifted)       :: 102 of 237
            Pseudo Genes (incomplete)         :: 130 of 237
            Pseudo Genes (internal stop)      :: 52 of 237
            Pseudo Genes (multiple problems)  :: 41 of 237
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13884
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267800-rid25106413.denovo.116"
                     /serotype="O 26:H11"
                     /isolate="2201S31241"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-01"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            complement(<1..1892)
                     /locus_tag="ACHOSC_RS13630"
                     /old_locus_tag="pgaptmp_002726"
     CDS             complement(<1..1892)
                     /locus_tag="ACHOSC_RS13630"
                     /old_locus_tag="pgaptmp_002726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease"
                     /protein_id="HGU4548639.1"
                     /translation="MSKVFICAAIPDELATREEGSVAVATAIEAGDERRARAKFHWQF
                     LEHYPAAQDCAYKFIVCEDKPGIPRPALDSWDAEYMQENRWDEESASFVPVETESDPM
                     NVTFDKLAPEVQNAVMVKFDTCENITVDMVISAQELLQEDMATFDGHIVEALMKMPEV
                     NAMYPELKLHAIGWVKHKCIPGAKWPEIQAEMRIWKKRREGERKETGKYTSVVDLARA
                     RANQQYTENSTGKISPVIAAIHREYKQTWKTLDDELAYALWPGDVDAGNIDGSIHRWA
                     KKEVIDNDREDWKRISASMRKQPDALRYDRQTIFGLVRERPIDIHKDPVALNKYICEY
                     LTTKGVFENEETDLGTVDVLQSSETQTDAVETEVSDIPKNETAPEAEPSVEREGPFYF
                     LFADKDGEKYGRANKLSGLDKALAAGATEITKEEYFARKNGTYTGLPQNVDTAEDSEQ
                     PEPIKVTADEVNKIMQAANISQPDADKLLAASRGEFVEGISDPNDPKWVKGIQTRDSV
                     NQNQHESERNYQKAEQNSPNALQNEPETKQPEPVAQQEVEKACTACGQTGGGNCPDCG
                     AVMGDATYQETFDEEYQVEVQEDDPEEMEGAEHPHKENTGGNQHHNSDNETGETADHP
                     IKVNGH"
     gene            complement(1986..2177)
                     /gene="ydfD"
                     /locus_tag="ACHOSC_RS13635"
                     /old_locus_tag="pgaptmp_002727"
     CDS             complement(1986..2177)
                     /gene="ydfD"
                     /locus_tag="ACHOSC_RS13635"
                     /old_locus_tag="pgaptmp_002727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysis protein YdfD"
                     /protein_id="HGU4548640.1"
                     /translation="MNSAFALVLTVFLVSGVPVDIAVSVHRTMQECMTAATEQKIPGN
                     CYPVDKVIHQDNIEIPAGL"
     gene            complement(2174..2362)
                     /gene="dicB"
                     /locus_tag="ACHOSC_RS13640"
                     /old_locus_tag="pgaptmp_002728"
                     /gene_synonym="ftsT"
     CDS             complement(2174..2362)
                     /gene="dicB"
                     /locus_tag="ACHOSC_RS13640"
                     /old_locus_tag="pgaptmp_002728"
                     /gene_synonym="ftsT"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division inhibition protein DicB"
                     /protein_id="HGU4548641.1"
                     /translation="MKTLLPNVNTSEGCFEIGVTISNPVFTEDAINKRKQERELLNKI
                     CIVSMLARLRLMPKGCAQ"
     gene            complement(3323..3424)
                     /gene="ydfB"
                     /locus_tag="ACHOSC_RS13645"
                     /old_locus_tag="pgaptmp_002729"
                     /pseudo
     CDS             complement(3323..3424)
                     /gene="ydfB"
                     /locus_tag="ACHOSC_RS13645"
                     /old_locus_tag="pgaptmp_002729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000344964.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="protein YdfB"
     gene            complement(3426..3581)
                     /gene="ydfA"
                     /locus_tag="ACHOSC_RS13650"
                     /old_locus_tag="pgaptmp_002730"
     CDS             complement(3426..3581)
                     /gene="ydfA"
                     /locus_tag="ACHOSC_RS13650"
                     /old_locus_tag="pgaptmp_002730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1391 family protein"
                     /protein_id="HGU4548642.1"
                     /translation="MDTIDLGNSESLVCGVFPNQDGTFTAMTYTKSKTFKTENGARRW
                     LERNSGE"
     gene            complement(3774..4181)
                     /locus_tag="ACHOSC_RS13655"
                     /old_locus_tag="pgaptmp_002731"
     CDS             complement(3774..4181)
                     /locus_tag="ACHOSC_RS13655"
                     /old_locus_tag="pgaptmp_002731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001003380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HGU4548643.1"
                     /translation="MNEKTLGQRIRERRKQVGLSQNGLSKAAGVSGSSISLWESDHTA
                     PRGQNLHRLAEVLQCSPTWILFGDEDKTPAPPVSLDSALDLSEDELEMLRLYRALPKS
                     EQQAQLSELRARVENFNRLFTELLEARKRNKHQ"
     gene            4259..4486
                     /locus_tag="ACHOSC_RS13660"
                     /old_locus_tag="pgaptmp_002732"
     CDS             4259..4486
                     /locus_tag="ACHOSC_RS13660"
                     /old_locus_tag="pgaptmp_002732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000476984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cro/CI family transcriptional regulator"
                     /protein_id="HGU4548644.1"
                     /translation="MFKSDVINFYGTKAKVAKAAGVDPSAVSQWQELVPEGRAMRLQE
                     ASGGELLYDPKVYDEYRKTKRAGRLNNENHS"
     gene            4470..4991
                     /locus_tag="ACHOSC_RS13665"
                     /old_locus_tag="pgaptmp_002733"
     CDS             4470..4991
                     /locus_tag="ACHOSC_RS13665"
                     /old_locus_tag="pgaptmp_002733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001578395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxin YdaT family protein"
                     /protein_id="HGU4548645.1"
                     /translation="MKITPEQAREALDAWICRPGMTQEQATILITEAFWALKERPNID
                     VQRVTDEGGAVDQRALGVNRVKIFERWKAIDTRDKREKFTALVPAIMEAIRINDFRLY
                     REISDGKSITYMIAGLNKEYGDVVESGLLFADPAVVDRETDELIEKAIAFKLAYRQQY
                     QQKAGWNYEPSFC"
     gene            4972..5937
                     /locus_tag="ACHOSC_RS13670"
                     /old_locus_tag="pgaptmp_002734"
     CDS             4972..5937
                     /locus_tag="ACHOSC_RS13670"
                     /old_locus_tag="pgaptmp_002734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001373617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4548646.1"
                     /translation="MSLLFAERPLVINTQLAMKIGLNEAIVLQQLHYWLRDTNSGMEC
                     DGVRWIYNTTEQWLEQFPFWSESTLKRAFASLKTLGLLRCEKLNKSKRDMTNFYTINY
                     GSELLDGGKLSESIGLKCAAPSGQNDTMEEVKMKRSIGSKRPNVIGSKWPDDLTENTT
                     EITTENKNTFRPEASQPDQQTAEQDFLTRNPDAVVFSAKKRQWGSREDLACAQWIWGR
                     IVNLYEQAASDDGEIMRPKEPNWTAWANDVRTMRMLDGRSHRQICEMFGRVQRDQFWV
                     KNIMSPSKLREKWDELVIRLGRSPVQRCVNHISEPDTEIPPGFRG"
     gene            5978..6397
                     /locus_tag="ACHOSC_RS13675"
                     /old_locus_tag="pgaptmp_002735"
     CDS             5978..6397
                     /locus_tag="ACHOSC_RS13675"
                     /old_locus_tag="pgaptmp_002735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001151196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF977 family protein"
                     /protein_id="HGU4548647.1"
                     /translation="MAKVFTQEEREKIKGQVVELVRQSGRETLRQLEAKTGATRYLMS
                     VLARELVASGDVYSSGYGLFPSEQARKDWQNARKKLSRAKVKKPAVVDPDLIWSLPDG
                     EIRRYDSRLNIICRECRKARRSRLLLTPSCNVPFEPF"
     gene            6452..7549
                     /locus_tag="ACHOSC_RS13680"
                     /old_locus_tag="pgaptmp_002736"
                     /pseudo
     CDS             6452..7549
                     /locus_tag="ACHOSC_RS13680"
                     /old_locus_tag="pgaptmp_002736"
                     /inference="COORDINATES: protein motif:HMM:NF033594.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS1202-like element ISEsa1 family transposase"
     gene            7622..7771
                     /gene="ydfW"
                     /locus_tag="ACHOSC_RS13685"
                     /old_locus_tag="pgaptmp_002737"
     CDS             7622..7771
                     /gene="ydfW"
                     /locus_tag="ACHOSC_RS13685"
                     /old_locus_tag="pgaptmp_002737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YdfW"
                     /protein_id="HGU4548648.1"
                     /translation="MDNARIDLRSKYYVKSKADHPWLTRRTQSHQQVKPPKLPKKKPD
                     PDKID"
     gene            complement(8208..8540)
                     /gene="flxA"
                     /locus_tag="ACHOSC_RS13690"
                     /old_locus_tag="pgaptmp_002738"
     CDS             complement(8208..8540)
                     /gene="flxA"
                     /locus_tag="ACHOSC_RS13690"
                     /old_locus_tag="pgaptmp_002738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FlxA-like family protein"
                     /protein_id="HGU4548649.1"
                     /translation="MSVTIQGNTSTVISNNSAPEGTSEIAKITRQIQVLTEKLGKISS
                     EEGMTTQQKKEMAALVQKQIESLRAQLEQLLRQQAEKKNKDATVQPDKKEEKKDDTNT
                     AGTIDIYV"
     gene            complement(8743..9048)
                     /gene="ydfV"
                     /locus_tag="ACHOSC_RS13695"
                     /old_locus_tag="pgaptmp_002739"
     CDS             complement(8743..9048)
                     /gene="ydfV"
                     /locus_tag="ACHOSC_RS13695"
                     /old_locus_tag="pgaptmp_002739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YdfV"
                     /protein_id="HGU4548650.1"
                     /translation="MSLQVSHYNMLRASHEGSQKVVVRTVITVRFVPGAAIAKSILYC
                     AGQLVFKESGHHLTGTDTIYFVTVRLHMHNSTYSSIFCFSMSVLLSRRRVSGDLTCR"
     gene            9073..9312
                     /gene="relB"
                     /locus_tag="ACHOSC_RS13700"
                     /old_locus_tag="pgaptmp_002740"
     CDS             9073..9312
                     /gene="relB"
                     /locus_tag="ACHOSC_RS13700"
                     /old_locus_tag="pgaptmp_002740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system antitoxin RelB"
                     /protein_id="HGU4548651.1"
                     /translation="MGSINLRIDDELKARSYAALEKMGVTPSEALRLMLEYIADNERL
                     PFKQTLLSDEDAELVEIVKERLRNPKPVRVTLDEL"
     gene            9312..9599
                     /gene="relE"
                     /locus_tag="ACHOSC_RS13705"
                     /old_locus_tag="pgaptmp_002741"
     CDS             9312..9599
                     /gene="relE"
                     /locus_tag="ACHOSC_RS13705"
                     /old_locus_tag="pgaptmp_002741"
                     /EC_number="3.1.26.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system mRNA interferase
                     RelE"
                     /protein_id="HGU4548652.1"
                     /translation="MAYFLDFDERALKEWRKLGSTVREQLKKKLVEVLESPRIEANKL
                     RGMPDCYKIKLRSSGYRLVYQVIDEKVVVFVISVGKRERSEVYSEAVKRIL"
     gene            9671..9826
                     /gene="hokD"
                     /locus_tag="ACHOSC_RS13710"
                     /old_locus_tag="pgaptmp_002742"
     CDS             9671..9826
                     /gene="hokD"
                     /locus_tag="ACHOSC_RS13710"
                     /old_locus_tag="pgaptmp_002742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin HokD"
                     /protein_id="HGU4548653.1"
                     /translation="MKQQKAMLIALIVICLTVIVTALVTRKDLCEVRIRTGQTEVAVF
                     TAYEPEE"
     gene            10043..10294
                     /gene="rem"
                     /locus_tag="ACHOSC_RS13715"
                     /old_locus_tag="pgaptmp_002743"
     CDS             10043..10294
                     /gene="rem"
                     /locus_tag="ACHOSC_RS13715"
                     /old_locus_tag="pgaptmp_002743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein Rem"
                     /protein_id="HGU4548654.1"
                     /translation="MMNIEELRKIFCEDGLYAVCVENGNLVSHYRIMCLRKNGAALIN
                     FVDARVTDGFILREGEFVTSLQALKEIGIKAGFSAFSGE"
     gene            10361..10639
                     /locus_tag="ACHOSC_RS13720"
                     /old_locus_tag="pgaptmp_002744"
     CDS             10361..10639
                     /locus_tag="ACHOSC_RS13720"
                     /old_locus_tag="pgaptmp_002744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4548655.1"
                     /translation="MARNAKYYNSDNSPVLACTHGRYSHAFKSEWFQHPPCTAEQAEW
                     LIHSYRRRGFEVKKALSLDYRHWIISVRLPYSERPPRASRTFQQRIWR"
     gene            10641..11690
                     /gene="ydfU"
                     /locus_tag="ACHOSC_RS13725"
                     /old_locus_tag="pgaptmp_002745"
     CDS             10641..11690
                     /gene="ydfU"
                     /locus_tag="ACHOSC_RS13725"
                     /old_locus_tag="pgaptmp_002745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416078.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF968 domain-containing protein"
                     /protein_id="HGU4548656.1"
                     /translation="MRVLLRPVLVPELGLVVLKPGRESIQIFHNPRVLVEPEPKSMRN
                     LPSGVVPAVRQPLAEDKTLLPFFSNERVIRAAGGVGALSDWLLRHVTSCQWPNGDYHH
                     TETVIHRYGTGAMVLCWHCDNQLRDQTSESLELLAQQNLTAWVIDVIRHAISGTQERE
                     LSLAELSWWAVCNQVVDALPEAVSRRSLGLPAEKICSVYRESDIVPGEQTATSILKQR
                     TKNLAPLPYAHQQQKSPQEKTVVSITVDPESPESFMKLPKRRRWVKEKYTRWVKTQPC
                     ACCGMPADDPHHLIGHGQGGMGTKAHDLFVLPLCRKHHNELHTDTVAFEDKYGSQLEL
                     IFRFIDRALAIGVLA"
     gene            11704..12456
                     /gene="ydfT"
                     /locus_tag="ACHOSC_RS13730"
                     /old_locus_tag="pgaptmp_002746"
     CDS             11704..12456
                     /gene="ydfT"
                     /locus_tag="ACHOSC_RS13730"
                     /old_locus_tag="pgaptmp_002746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416077.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antitermination protein"
                     /protein_id="HGU4548657.1"
                     /translation="MNLEALPKYYSPKSPKLSDDAPATGTGCLTITDVMAAQGMVQSK
                     APLGLALFLAKVGVQDPQFAIEGLLNYAMALDNPTLNKLSEEIRLQIIPYLVNFAFAD
                     YSRSAASKARCEHCSGTGFYNVLREVVKHYRRGESVIKEEWVKELCQHCHGKGEVSTA
                     CRGCKGKGIVLDEKRTRFHGVPVYKICGRCNGNRFSRLPTTLARRHVQKLVPDLTDYQ
                     WYKGYADVIGKLVTKCWQEEAYAEAQLRKVTR"
     gene            complement(12734..12823)
                     /locus_tag="ACHOSC_RS13735"
                     /old_locus_tag="pgaptmp_002747"
     CDS             complement(12734..12823)
                     /locus_tag="ACHOSC_RS13735"
                     /old_locus_tag="pgaptmp_002747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4548658.1"
                     /translation="MNNPVCLDDWLIGFKSLCCTLAVIALLII"
     gene            complement(12878..13090)
                     /gene="cspF"
                     /locus_tag="ACHOSC_RS13740"
                     /old_locus_tag="pgaptmp_002748"
     CDS             complement(12878..13090)
                     /gene="cspF"
                     /locus_tag="ACHOSC_RS13740"
                     /old_locus_tag="pgaptmp_002748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold shock-like protein CspF"
                     /protein_id="HGU4548659.1"
                     /translation="MSRKMTGIVKTFDGKSGKGLITPSDGRIDVQLHVSALNLRDAEE
                     ITTGLRVEFCRINGLRGPSAANVYLS"
     gene            13391..13606
                     /gene="cspB"
                     /locus_tag="ACHOSC_RS13745"
                     /old_locus_tag="pgaptmp_002749"
     CDS             13391..13606
                     /gene="cspB"
                     /locus_tag="ACHOSC_RS13745"
                     /old_locus_tag="pgaptmp_002749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold shock-like protein CspB"
                     /protein_id="HGU4548660.1"
                     /translation="MSNKMTGLVKWFNADKGFGFISPVDGSKDVFVHFSAIQNDNYRT
                     LFEGQKVTFSIERGAKGPAAANVIITD"
     gene            complement(13596..>13884)
                     /locus_tag="ACHOSC_RS13750"
                     /old_locus_tag="pgaptmp_002750"
     CDS             complement(13596..>13884)
                     /locus_tag="ACHOSC_RS13750"
                     /old_locus_tag="pgaptmp_002750"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4548661.1"
                     /translation="LWCFLWESSTYALCFAMPAVSFCCETIHAANLHFTTMVSILFLI
                     KILPNMLAIVSCIGPPYFLLKRSNIHYSVRISTQASAFFVIVYRRAMNFNQ"
BASE COUNT         3627 a         3027 c         3427 g         3803 t
ORIGIN      
        1 tgatgaccgt tcaccttaat tgggtgatct gccgtctcgc cagtttcatt atcgctattg
       61 tgatgctgat tgccgccagt gttctccttg tgtggatgtt cagcgccttc catttcctcc
      121 ggatcatctt cctgaacttc aacctgatac tcttcatcga atgtttcttg gtatgttgcg
      181 tcgcccatca ccgcgccaca atcagggcag ttgccgccgc cggtctgacc gcaggcggtg
      241 caggcttttt ccacttcctg ttgcgccact ggttcaggct gtttcgtttc tggctcgttt
      301 tgtaacgcat ttggactgtt ttgttccgct ttttggtagt tccgttccga ttcatgctgg
      361 ttctggttca cagaatcgcg ggtctggatc cccttaaccc atttcggatc attcgggtca
      421 ctaatccctt caacaaattc accacgtgat gcagcaagca acttatcggc gtcaggctgg
      481 ctgatattgg ctgcctgcat aattttgttt acttcgtcag cggtaacttt tatcggctct
      541 ggttgttctg aatcttcagc ggtatctaca ttttgcggta agcccgtgta tgtgccattt
      601 tttcgggcaa aatattcttc ttttgtgatt tcagtggcgc cagcagccag tgccttatcc
      661 agaccagaaa gtttgtttgc gcgaccgtat ttttctccgt ccttatctgc gaagaggaaa
      721 tagaacggcc cctcacgctc tacagatggt tcagcttccg gcgcggtttc attttttggg
      781 atatcagata cctcagtttc cactgcatca gtttgtgttt ctgatgactg gagaacatca
      841 acagtgccca ggtctgtttc ttcattctca aacacgccct ttgtcgtcag gtattcgcag
      901 atatatttgt tcagtgctac gggatctttg tgaatgtcga tcggacgctc acggacaagg
      961 ccaaaaatag tttggcggtc gtagcgaagg gcatcaggct gtttgcgcat tgatgccgag
     1021 atacgcttcc agtcttcgcg gtcgttgtcg ataacttctt tttttgccca gcgatggatg
     1081 ctgccgtcaa tgtttccggc atccacatca ccaggccaga gagcgtaggc cagttcgtca
     1141 tccagtgttt tccatgtctg cttgtattcg cgatgaatgg cagcaatgac cgggctgatt
     1201 tttcctgttg aattttcagt gtactgttga ttggctctgg cgcgggcgag atcaacaaca
     1261 gacgtgtatt ttccggtttc cttgcgttca ccttcgcgac gttttttcca gatgcgcatc
     1321 tctgcctgaa tttcgggcca tttagcacca ggaatacatt tatgcttaac ccacccaatg
     1381 gcgtgcaact taagctccgg atacatggcg ttaacttctg gcattttcat caacgcttca
     1441 acgatatgtc cgtcgaatgt tgccatgtct tcctgcaaca attcctgtgc gctaataacc
     1501 atatcaacgg tgatgttttc acatgtgtcg aacttaacca tgacagcgtt ctgtacttca
     1561 ggggccagct tgtcaaaagt gacgttcatc ggatcggatt cagtctcaac cgggacaaaa
     1621 gaagcagact cctcatccca gcggttttcc tgcatatatt cagcatccca tgaatcgagg
     1681 gcagggcggg gtatgccagg tttatcctcg cagacaataa atttataagc gcagtcctga
     1741 gcagccggat aatgttccag gaactgccag tgaaattttg ctcgagcacg gcgttcgtcg
     1801 ccagcttcaa tggctgtggc tacagccaca gagccttctt cccttgttgc cagttcgtca
     1861 ggaatagcgg cgcaaataaa gactttactc attttgtttt aacctcatga cagatttaag
     1921 gatgaacaaa tccctgccat tgctggcata taagaatgaa accggatatt tattacggaa
     1981 ctgttttaaa gacctgccgg gatttcgata ttatcctggt gaataacttt atcgaccggg
     2041 taacagttac cgggaatttt ctgttcggtt gctgcagtca tacactcctg cattgtcctg
     2101 tgaacactga ctgcaatatc aactggcact ccggaaacaa gaaaaactgt cagaacaagc
     2161 gcaaatgctg aattcattgt gcacatcctt ttggcatcag acgtaaacga gccagcattg
     2221 aaacaatgca tattttattt aatagctccc gttcttgttt tctcttgtta atggcatctt
     2281 cagtaaatac agggttactg atagtgacac caatttcaaa acaaccttca gacgtattaa
     2341 cgtttggtaa taacgttttc attatcgcgt cctcaacaat gaattttgtg atgcagtgcc
     2401 tggtgcctcc aggtgacgtt aaccagttaa caattaacgt cggatacaga gaatccaccc
     2461 ataacactgt ttttggtttt aactgttccg cgtgcgctta gccgtattca ccgcatcaca
     2521 aaattcactt taaaaagggc ggcagagcag tcacggagta aaactgatac cgccaaacgt
     2581 caccagaaaa ttgataacag agggcgttgc agcggggttg tcacttaagc gtatggtcaa
     2641 cctgacaacc cggtgtcctc aacggggaag gaataacccc gccatactta ccgccgcgcc
     2701 attttgcggg ttgccacaac cggaagcgca cggtcgaatt aaatttaacg acaccgtaca
     2761 gtgagacgaa cttcgccgtg cgctttcgtg ttgtgtgcct gcttttaacc acgtcaggcg
     2821 aggtggtatc cttaaaatca ccacagtttt aaggattcat taagcaatgt cgcaaccacc
     2881 aataaatccg cttaagaaca tgaaaattga ttactggtat aaagcgctta cagttgttgg
     2941 cgctgcgttg tttgtcttta atggaacgtc tttttttgac agatatcccg ttgttccatt
     3001 gggttttttg tcctccggca tcttttttat tggtttgggg gagtggatta atcaccctct
     3061 catagtgaga tttattggtc ctggagtttg gactcgtgga tataatcgtt cttcgtgcgc
     3121 actcggtatc atcttcgaca tacttggttg tttcctgatt gttacaggag tcgtcaagtt
     3181 cttctgatgt aaaaccgcaa atggggcacg taacgggaat tttgaaaagc gtttctccgg
     3241 gttccagaac aaaattttct gcggtctgat tttgcttctc atatttgtgc tccgcgtcat
     3301 tgtgagagca cattcttatt ctgagtgcct gtttaaactc actgaagctg agagcttctt
     3361 cgccttcggc aaggccttcg aagtattctt cgtaagcctt ttccatgatt gtgttgaaat
     3421 ccatatcact cacctgagtt tctttccagc cagcgacggg caccattttc ggttttaaac
     3481 gttttgcttt tggtatacgt catcgcggtg aacgtgccgt cctggttggg gaacacgcca
     3541 cataccagag attcgctgtt gccaagatcg atagtatcca tgctgacctc atttcccctt
     3601 aacgccgggg tagcggaact gtttgctgag aacaccgtgc ggtgtcttga tggaggtaat
     3661 ttagttttct cattattttt cgtcaaggtt ttttgatgag aaaactcaag tgttggcgca
     3721 agataaagcc aatacattga aatgtaaggc tttaaaattt tgtgaaggga tgattattga
     3781 tgcttgttgc gtttgcgagc ctctaatagc tcggtgaaca ggcgattaaa attctcaacg
     3841 cgggcgcgga gttcgctgag ttgtgcttgt tgctctgatt taggaagtgc gcgatacaaa
     3901 cgcagcatct ctaactcatc ttccgataag tctaaggcac tatcgagtga aacaggggga
     3961 gctggtgttt tgtcctcgtc gccaaacagt atccaagttg gtgaacattg caatacctcg
     4021 gcgaggcgat gcaaattttg cccgcgcggg gctgtatggt cactttccca taatgaaatt
     4081 gatgagccag atacgccagc agctttgctt aaaccgtttt gacttaagcc tacctgctta
     4141 cgtctttctc taatgcgttg acctaaagtt ttctcgttca tatttagata tcttaataac
     4201 ccttgacttg agattccttg agtgattagc attgagaaaa ctcaatattg gaggtgcgat
     4261 gtttaaatca gacgtaatta atttttacgg tacgaaagcc aaagtagcga aagctgctgg
     4321 cgttgaccca tctgctgttt ctcaatggca agagctggtt cctgaaggtc gcgcgatgcg
     4381 tctacaggag gcatctggcg gcgagcttct gtatgatccc aaggtttatg acgaatatcg
     4441 taagacgaag cgggcggggc ggttgaacaa tgaaaatcac tcctgaacag gctcgtgagg
     4501 ctctggatgc ctggatatgt cgaccaggaa tgacacagga gcaggcgacg atattaatca
     4561 ctgaagcatt ctgggctttg aaagagcgcc cgaacatcga tgttcagcgt gtcacagatg
     4621 aaggtggcgc ggttgatcag cgagcacttg gcgttaatcg agtgaagata ttcgaacgct
     4681 ggaaggctat cgacaccagg gataagcgtg aaaagttcac ggcgctagtg cctgcaatta
     4741 tggaggctat tcggattaat gatttcaggt tgtatcgtga aattagtgac ggaaaaagca
     4801 tcacgtacat gatcgccggg ttaaacaaag aatatggcga tgtggtggag tccggactgc
     4861 tttttgctga tcctgccgta gtggatcgtg aaactgacga acttatagaa aaagcaattg
     4921 ctttcaaact tgcgtatcga cagcaatacc aacaaaaagc tggatggaat tatgagcctt
     4981 ctttttgctg aacgcccact ggttataaac acacagcttg cgatgaagat tggcttaaat
     5041 gaagccattg tgttgcagca gttgcattac tggttgagag ataccaattc cggcatggaa
     5101 tgtgatggtg ttcgctggat ttacaacaca acggaacaat ggctggaaca gttcccattc
     5161 tggtcagagt caacgttaaa gcgcgcgttt gcaagtctga aaacgctggg gcttttgcgt
     5221 tgtgaaaagc tcaataaatc aaagcgcgat atgaccaatt tctacacgat caactacggg
     5281 agcgagcttt tagatggtgg caaattgagc gaatccatcg gtttaaaatg cgccgctcca
     5341 tcaggtcaaa atgacacgat ggaagaggtc aaaatgaaac gctccattgg ttcaaaacga
     5401 cccaatgtca tcgggtcaaa atggcctgat gatcttacag agaatacaac agagattact
     5461 acagagaata aaaacacttt tcgtccggaa gcttcgcaac cggaccagca gaccgctgaa
     5521 caggattttt taacccggaa ccccgacgcg gttgtgttta gtgcgaaaaa acgccagtgg
     5581 ggtagcaggg aggatttggc gtgtgcgcag tggatttggg ggcggatcgt gaacctttac
     5641 gaacaggctg ccagcgacga tggagagatc atgcgaccaa aagagcctaa ctggacagcc
     5701 tgggccaatg acgtgcgcac aatgcggatg ctggatggca gaagccacag acaaatttgc
     5761 gaaatgtttg gtcgggtaca gcgagatcaa ttctgggtaa aaaacatcat gagcccgtca
     5821 aaactccgcg aaaaatggga cgaactggtc atccgcctgg gacgttcacc tgtacagcgt
     5881 tgtgtgaatc atatttctga accggataca gaaattccgc ctggtttcag gggataagtg
     5941 ttgattttag ggtataaagt aatgttcagg aggacttgtg gcaaaagttt ttacacaaga
     6001 agagcgggaa aaaattaaag ggcaggtggt ggaactcgta cgccagagtg ggcgcgagac
     6061 gttacgacaa ctggaagcta aaactggggc aacaagatat ctgatgagcg ttctggccag
     6121 agagctggtt gccagtggcg atgtatacag ctctggttac gggttattcc cgtctgaaca
     6181 ggcgcgtaag gactggcaaa acgcccgcaa aaaactctcg agggcaaagg tgaagaaacc
     6241 tgctgtggtt gatccggacc ttatctggtc attacctgac ggagaaatac gtcgctacga
     6301 cagtcgtcta aacataatct gtcgcgagtg ccggaaggca aggagaagca ggctattgtt
     6361 aacaccaagc tgtaatgtcc cctttgaacc attctaaaat gtccccagac aattctctgg
     6421 gggatttttc atgatcaaag agactgttac gatgagtcat aaggaactcc accgacttca
     6481 gattattcag gagtcaatta atcgacatat tactcaggaa caagctgcgg cacgcattgg
     6541 catttctatt cggcaggtta aacgtctggt gcaacggtat agaaatgaag ggccttctgg
     6601 tctggtttcc caccgacgtg gaaagcgtcc taataattcc ttttctactg aattcagagc
     6661 aacagtaatt tcactcctca aaggccgtta cgctgatttt ggacctacgt ttgcgtgcga
     6721 aaaattgcgc gagatacacg gtttatcttt atccgttgaa actctcagaa agtggatgat
     6781 agaagagggg ttatggcgtg aacgccgtcg taaaattgcc cgtatatatc aacgccgcat
     6841 gcgacgacca tcttacggtg aactgatcca gattgatggc tcacctcatg actggtttga
     6901 aaatcgaggc cccagatgta cactgatcgt tttcattgat gatgccacca gtgcgttgat
     6961 ggcgttgcgt tttgtgcctg ctgaaacaac ccgggcttac atggaaaccc tccggggtta
     7021 ccttaatgat catggcgtac cgctcgctct ctactctgat agacacagta tattcagggt
     7081 aaataaccca gagcgggaag gtgagctgac ccagttcact cgtgcgataa agacactggg
     7141 catcgagcca atccatgcca acagcccgca ggcaaaaggg cgggtagagc gcgccaatca
     7201 gacactacag gacaggctgg tcaaagaaat gcggcttcag aatatcagtg atattgaaac
     7261 agcaaatgca tggttgccga cctttattga agcctataac aaccggttcg ctacgtcgcc
     7321 tcgtactact gataatgctc atcttgatgt gcaccattct gaagaggaac tgggttatat
     7381 cttcagccta caggcgaagc gcgttctgtc taaaaatctc actttccagt acaaaagcag
     7441 tgcgtttcag gtacgcagtg agggccgggg atatcgactt aggcattcgg ttgttactgt
     7501 atgtgagaac tttgacggtg aaattaacgt tctgtatgac gggaaagcgc tgggctggga
     7561 aaagtatgtt gatggcccgg agcctatacc actggatgat gaaaagagtg tccatgaacg
     7621 agtggataat gcccgtattg atttacgctc aaaatactat gttaaatcta aagctgacca
     7681 tccctggctt acgcgccgaa cgcaaagtca tcagcaagtt aagcccccga agttacctaa
     7741 aaagaagcct gatcccgata aaatagattg aaaccaagat cgattcggtt gagtgcatat
     7801 ccattcatag ggtagattct taagtcgcgt ttctgatgtt cattttcggg tggtttgtta
     7861 cttgttttac cggggatatg ccagaaacgc gctgagtcag tctgggcggt gcgcgtaatg
     7921 aggcgttatg gtaaatagcc tatgctaatg tccgctaaga gcaagaagcg gaagttggca
     7981 gttttgtgga ctgtccccac aaaagtgact acagaaatag ttgcaattca taattgatca
     8041 tgggttgtca gttaaactcg tggcgattta aatagactaa ttgggagtgc gtccattact
     8101 tatatcttgt aatgttaact atcagaaatg atacaaagat aatatgtctt taaagaaaag
     8161 gctgatggcg aaaagtggcc cgatgagggc cacaatacgg ctgtcactta gacgtaaata
     8221 tcaatggtgc cagcggtatt tgtatcgtct tttttctctt cttttttatc aggctgaact
     8281 gtcgcgtctt tattcttttt ctctgcctgc tgccttaaca actgctccag ttgagccctg
     8341 aggctttcaa tttgcttctg taccaatgca gccatttctt ttttctgctg tgtcgtcatc
     8401 ccctcttccg atgagatttt cccaagcttt tcagtcagca cctgaatttg tcttgtgatt
     8461 ttggctattt ctgatgttcc ttccggggcg gagttgtttg aaataacggt tgaggtattt
     8521 ccctgaattg tgacagacat agatttctcc ttttaaaaaa gcactatcgg catgcacaaa
     8581 aaaatcttta atcgtatttc ttgtgtcatt aattgtttga tgttcagatt gttttcctcg
     8641 cgggctggcg cgcctcagaa agtaaagctt gttgacaggg gtaaacgttc ggcaataatt
     8701 ttctgccgca tgcgggtgtt gcataaaacg tgctacgttc ctttatcgac aggtcaggtc
     8761 accgctcacc cgccgacgag aaagcaacac tgacatgcta aagcaaaaaa tagatgaata
     8821 agttgagttg tgcatatgta gcctgaccgt cacaaagtat atggtgtctg taccagtaag
     8881 atgatggccg gactctttaa aaacgagctg acctgcacaa tacaggatgg acttagcaat
     8941 ggctgctcct ggcacaaagc ggacagtgat caccgttctt acgactactt tctgacttcc
     9001 ttcgtgactt gccctaagca tgttgtagtg cgatacttgt aatgacattt gtaattacaa
     9061 gaggtgtaag acatgggtag cattaacctg cgtattgacg atgaacttaa agcgcgttct
     9121 tacgccgcgc ttgaaaaaat gggtgtaact ccttctgaag cgcttcgtct catgctcgag
     9181 tatatcgctg acaatgaacg cttgccgttc aaacagacac tcctgagtga tgaagatgct
     9241 gaacttgtgg agatagtgaa agaacggctt cgtaatccta agccagtacg tgtgacgctg
     9301 gatgaactct gatggcgtat tttctggatt ttgacgagcg ggcactaaag gaatggcgaa
     9361 agctgggctc gacggtacgt gaacagttga aaaagaagct ggttgaagta cttgagtcac
     9421 cccggattga agcaaacaag ctccgtggta tgcctgattg ttacaagatt aagctccggt
     9481 cttcaggcta tcgccttgta taccaggtta tagacgagaa agttgtcgtt ttcgtgattt
     9541 ctgttgggaa aagagaacgc tcggaagtat atagcgaggc ggtcaaacgc attctctgaa
     9601 ccaaagcatg acatctctgt ttcgcaccga aggtgacact tctgctttgc gttgacagga
     9661 gaagcaggct atgaagcagc aaaaggcgat gttaatcgcc ctgatcgtca tctgtttaac
     9721 cgtcatagtg acggcactgg taacgaggaa agacctctgc gaggtacgaa tccgaaccgg
     9781 ccagacggag gtcgctgtct tcacagctta cgaacctgag gagtaagaga cccggcgggg
     9841 gagaaatccc tcgccacctc tgatgtggca ggcatcctca acgcacccgc acttaacccg
     9901 cttcggcggg tttttgtttt tattttcaac gcgtttgaag ttctggacgg tgccggaata
     9961 gaatcaaaaa tacttaagta gcgcgcaggg ataagaggga tggtccctta aaggggagag
    10021 ctaattatcc ggaaggattc tgatgatgaa catcgaagaa ctgcgtaaaa ttttttgtga
    10081 agatggcctc tatgctgtgt gcgttgaaaa tggaaatctt gttagtcatt accgcattat
    10141 gtgtttgcga aagaatgggg ctgcgttaat taattttgtg gatgctcggg tcacggacgg
    10201 atttatcttg cgcgaaggtg agtttgtcac ttcattacag gcattgaaag agatcggaat
    10261 aaaagctggc ttttctgctt tttcaggaga ataaactcat ctacaatctt gcgcggggct
    10321 gaactcccgc tgagtaacac cgtgccaccg gagaaaaccg atggcacgca acgcaaaata
    10381 ttacaattct gataattcgc ccgttcttgc ctgcacgcac gggcggtatt ctcacgcatt
    10441 caagtctgaa tggttccagc accctccatg cactgcagaa caggccgaat ggctgattca
    10501 ttcttaccgc aggcgcgggt tcgaggttaa gaaagctctc agtctcgact atcggcactg
    10561 gataatctct gtcaggctgc cttattccga acgcccacca cgtgcgtccc gcactttcca
    10621 gcaacggatc tggaggtaac gtgcgggtat tacttagacc tgttctggtg cctgagcttg
    10681 ggctggtggt ccttaagccg ggccgtgaat ccatacagat atttcataat cctcgagtgc
    10741 tggtggaacc ggaaccaaaa agcatgcgta atctgccatc cggagtcgtt cctgccgttc
    10801 gccagccgct ggcggaagac aaaacattgc tgccgttttt tagtaacgaa cgggtgattc
    10861 gtgctgctgg cggcgttggc gcattgtccg actggctatt acgtcatgtt acatcctgcc
    10921 agtggcctaa tggcgattac catcacactg aaacagtcat tcaccgttat ggtaccggcg
    10981 caatggtgtt gtgctggcac tgcgacaacc aactgcgtga ccagacatcg gaatcactgg
    11041 agctgcttgc tcaacaaaat ctgacagcat gggtgattga cgtcatccgt cacgcaataa
    11101 gcggtacgca ggagcgggaa ttatctttgg ctgaattatc ctggtgggcg gtctgcaatc
    11161 aggtggtgga tgcactacct gaggctgtat cgcgtcgttc gctgggatta ccagcggaaa
    11221 aaatctgctc ggtgtaccgc gaaagcgaca tcgtaccggg agagcagacc gccaccagca
    11281 tattgaaaca acgcacaaaa aatcttgcac cgttgcctta cgcccaccag caacaaaaat
    11341 caccacagga aaagacggtg gtaagcatca ccgttgatcc agagtctccg gaatctttca
    11401 tgaagctgcc taaacgtcgc cgctgggtta aggagaaata cacacgttgg gttaagacac
    11461 agccgtgtgc ttgctgcggt atgccagccg acgatccgca tcatctgatt ggtcacgggc
    11521 agggcggaat gggaacaaaa gcacatgatc tctttgtgtt gcctttgtgc agaaagcatc
    11581 acaacgagct gcatacggat acagtggcat ttgaagataa gtatggctcc caactggagc
    11641 tgatatttcg ttttatcgat cgcgcgctgg caattggcgt actggcgtaa gtggagaacg
    11701 agcatgaacc ttgaagcctt accaaaatat tactccccaa aatctccaaa attgagcgat
    11761 gacgctccag cgacaggcac cggttgttta acaattacgg atgtaatggc agcgcagggg
    11821 atggtgcagt cgaaagcacc acttgggttg gccttatttc tggcaaaagt tggtgttcag
    11881 gaccctcagt ttgcgattga aggcctgcta aattacgcga tggcactgga taacccgaca
    11941 ttgaacaaat tgagtgaaga aatccggtta cagattattc cttacctcgt gaattttgcc
    12001 tttgctgatt actccaggtc tgcggcaagt aaggctcgct gtgagcattg ttcaggtacg
    12061 ggattttata atgtattgcg cgaagtggtg aaacactacc gacgcgggga atctgtaatc
    12121 aaggaagaat gggtgaagga actatgtcag cattgccatg gtaagggtga agtcagcaca
    12181 gcgtgcagag ggtgtaaggg taaagggatt gttctggatg aaaaaagaac ccggtttcat
    12241 ggcgtaccgg tatataagat ttgtgggcgt tgtaatggaa accggtttag tcgtttaccg
    12301 accacgctgg cacgacgtca tgtccagaag ctggtaccag acctgaccga ttatcagtgg
    12361 tataaggggt atgcggacgt cattggtaaa ctggtaacaa agtgctggca ggaagaagca
    12421 tacgcggaag cgcaattgag gaaggtgacg agataaatga tttttgctga agatggcgac
    12481 atgatgtttg catttttcaa aaaatatgga taaaattttt tcaacgatgg gctttgtata
    12541 cccgacgtta agaaaaagta gaaaacccgc tgatgagcgg gttttgtgct ttaaatgggg
    12601 caatggtaat gttgaatctc atcccgggac tcatgtctgt taacttatta tttagctggt
    12661 gacttggtta tttgcctgat gtttaaaatg ttttcttcca gtacaatgtc cctaaacaca
    12721 atgagtctgc ttattatatt attagcagag ctattacggc caaagtacag cataagcttt
    12781 taaagccaat caaccagtca tcaagacaga cggggttatt cataaaaact ctccatgtgt
    12841 gatccgatgg ggcctgaaat taaagcttta atatagctca tgaaaggtaa acattggcag
    12901 ctgaagggcc acgcagacca tttatccggc aaaattccac gcgtaatccg gtggtaattt
    12961 cttctgcatc gcggagattg agcgctgaaa catgaagctg gacatcgata cgaccatcgg
    13021 atggggtgat aagacccttg ccgcttttgc cgtcaaaggt tttgacaatt cctgtcattt
    13081 tacgggacaa aaaaattcct taatactgat aacttggcgc actatacaca cgttcctgaa
    13141 gaaagctata gttttttgat ggggttgaag atggctggat gtctaaaata aacattgctt
    13201 catatgttca actatgcgtt aatgattgcg tcggtttgaa gaacagacga tatacgaagt
    13261 agtttactaa agcagttctc atttcaggtg ttattcactt attccttctt tgagtctctc
    13321 caattaagta cgaagtcgtt tctgttatgc aaaccattta tgccgaaagg ctcaagttaa
    13381 ggaatgtaga atgtcaaata aaatgactgg tttagtaaaa tggtttaacg ctgataaagg
    13441 tttcggcttt atttctcctg ttgatggtag taaagatgtg tttgtgcatt tttctgcgat
    13501 tcagaatgat aattatcgaa ccttatttga aggtcaaaag gttaccttct ctatagagag
    13561 aggtgctaaa ggtcctgcag cagcaaatgt catcattact gattaaaatt catcgctcgt
    13621 ctgtatacga taacgaagaa ggctgatgcc tgagtagaga tacggacaga gtagtgaata
    13681 ttggatctct ttaataaaaa gtaaggaggt ccaatacatg aaacaatggc tagcatattt
    13741 ggcaaaatct taatcaggaa aagtatgcta accattgtgg tgaagtgcag gtttgctgca
    13801 tgaatagttt cacagcagaa gctaactgct ggcatcgcaa aacaaagtgc gtaagtggat
    13861 gactcccaca aaaagcacca caat
//
LOCUS       DAZKHF020000116        13524 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2201S31241
            SAMN43267800-rid25106413.denovo.117, whole genome shotgun sequence.
ACCESSION   DAZKHF020000116 DAZKHF020000000
VERSION     DAZKHF020000116.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267800
            Sequence Read Archive: SRR30307263
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 13524)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13524)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 13524)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350581.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350581.2
            Genome Coverage       :: 151x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:21:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,652
            CDSs (total)                      :: 5,557
            Genes (coding)                    :: 5,320
            CDSs (with protein)               :: 5,320
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 237
            CDSs (without protein)            :: 237
            Pseudo Genes (ambiguous residues) :: 0 of 237
            Pseudo Genes (frameshifted)       :: 102 of 237
            Pseudo Genes (incomplete)         :: 130 of 237
            Pseudo Genes (internal stop)      :: 52 of 237
            Pseudo Genes (multiple problems)  :: 41 of 237
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13524
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267800-rid25106413.denovo.117"
                     /serotype="O 26:H11"
                     /isolate="2201S31241"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-01"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            complement(35..187)
                     /locus_tag="ACHOSC_RS13755"
                     /old_locus_tag="pgaptmp_002751"
     CDS             complement(35..187)
                     /locus_tag="ACHOSC_RS13755"
                     /old_locus_tag="pgaptmp_002751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hok/Gef family protein"
                     /protein_id="HGU4548662.1"
                     /translation="MLTKYALVAIIVLCCTVLGFTLMVGDSLCELSIRERGMEFKAVL
                     AYESKK"
     gene            complement(452..1186)
                     /gene="cysH"
                     /locus_tag="ACHOSC_RS13760"
                     /old_locus_tag="pgaptmp_002752"
     CDS             complement(452..1186)
                     /gene="cysH"
                     /locus_tag="ACHOSC_RS13760"
                     /old_locus_tag="pgaptmp_002752"
                     /EC_number="1.8.4.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708560.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004604 - phosphoadenylyl-sulfate
                     reductase (thioredoxin) activity [Evidence IEA];
                     GO_process: GO:0019379 - sulfate assimilation,
                     phosphoadenylyl sulfate reduction by
                     phosphoadenylyl-sulfate reductase (thioredoxin) [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoadenosine phosphosulfate reductase"
                     /protein_id="HGU4548663.1"
                     /translation="MSKLDLNALNELPKVDRILALAETNAELEKLDAEGRVAWALDNL
                     PGEYVLSSSFGIQAAVSLHLVNQIHPDIPVILTDTGYLFPETYRFIDELTDKLKLNLK
                     VYRATESAAWQEARYGKLWEQGVEGIEKYNDINKVEPMNRALKELNAQTWFAGLRREQ
                     SGSRANLPVLAIQRGVFKVLPIIDWDNRTIYQYLQKHGLKYHPLWDEGYLSVGDTHTT
                     RKWEPGMSEEETRFFGLKRECGLHEG"
     gene            complement(1260..2972)
                     /gene="cysI"
                     /locus_tag="ACHOSC_RS13765"
                     /old_locus_tag="pgaptmp_002753"
     CDS             complement(1260..2972)
                     /gene="cysI"
                     /locus_tag="ACHOSC_RS13765"
                     /old_locus_tag="pgaptmp_002753"
                     /EC_number="1.8.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001290736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009337 - sulfite reductase complex
                     (NADPH) [Evidence IEA];
                     GO_function: GO:0004783 - sulfite reductase (NADPH)
                     activity [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0000103 - sulfate assimilation [Evidence
                     IEA];
                     GO_process: GO:0008652 - amino acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="assimilatory sulfite reductase (NADPH)
                     hemoprotein subunit"
                     /protein_id="HGU4548664.1"
                     /translation="MSEKHPGPLVVEGKLTDAERMKLESNYLRGTIAEDLNDGLTGGF
                     KGDNFLLIRFHGMYQQDDRDIRAERAEQKLEPRHAMLLRCRLPGGVITTKQWQAIDKF
                     AGENTIYGSIRLTNRQTFQFHGILKKNVKPVHQMLHSVGLDALATANDMNRNVLCTSN
                     PYESQLHAEAYEWAKKISEHLLPRTRAYAEIWLDHEKVATTDEEPILGQTYLPRKFKT
                     TVVIPPQNDIDLHANDMNFVAIAENGKLVGFNLLVGGGLSIEHGNKKTYARTASEFGY
                     LPLEHTLAVAEAVVTTQRDWGNRTDRKNAKTKYTLERVGVETFKAEVERRAGIKFEPI
                     RPYEFTGRGDRIGWVKGIDDNWHLTLFIENGRILDYPGRPLKTGLLEIAKIHKGDFRI
                     TANQNLIIAGVPESEKAKIEKIAKESGLMNAVTPQRENSMACVSFPTCPLAMAEAERF
                     LPSFIDNIDNLMAKHGVSDEHIVMRVTGCPNGCGRAMLAEVGLVGKAPGRYNLHLGGN
                     RIGTRIPRMYKENITEPEILASLDELIGRWAKEREAGEGFGDFTVRAGIIRPVLDPAR
                     DLWD"
     gene            complement(2972..4771)
                     /gene="cysJ"
                     /locus_tag="ACHOSC_RS13770"
                     /old_locus_tag="pgaptmp_002754"
     CDS             complement(2972..4771)
                     /gene="cysJ"
                     /locus_tag="ACHOSC_RS13770"
                     /old_locus_tag="pgaptmp_002754"
                     /EC_number="1.8.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004783 - sulfite reductase (NADPH)
                     activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0000103 - sulfate assimilation [Evidence
                     IEA];
                     GO_process: GO:0019344 - cysteine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent assimilatory sulfite reductase
                     flavoprotein subunit"
                     /protein_id="HGU4548665.1"
                     /translation="MTTQVPLSALLPLNPEQLARLQAATTDLTPTQLAWVSGYFWGVL
                     NQQPAALAATPAPAAEMPGITIISASQTGNARRVAEALRDDLLAAKLNVKLVNAGDYK
                     FKQIASEKLLIVVTSTQGEGEPPEEAVALHKFLFSKKAPKLENTAFAVFSLGDSSYEF
                     FCQSGKDFDSKLAELGGERLLDRVDADVEYQAAASEWRARVVDALKSRAPVAAPSQSV
                     ATGAVNEIHTSPYSKDAPLVASLSVNQKITGRNSEKDVRHIEIDLGDSGLRYQPGDAL
                     GVWYQNDPALVKELVELLWLKGDEPVTVEGKTLPLNEALQWHFELTVNTANIVENYAT
                     LTRSETLLPLVGDKAKLQHYAATMPIVDMVRFSPAQLDAEALINLLRPLTPRLYSIAS
                     SQAEVENEVHVTVGVVRYDVEGRARAGGASSFLADRVEEEGEVRVFIEHNDNFRLPAN
                     PETPVIMIGPGTGIAPFRAFMQQRAAGEAPGKNWLFFGNPHFTEDFLYQVEWQRYVKD
                     GVLTRIDLAWSRDQKEKVYVQDKLREQGAELWRWINDGAHIYVCGDANRMAKDVEQAL
                     LEVIAEFGGMDTEAADEFLSELRVERRYQRDVY"
     gene            5090..5452
                     /gene="queD"
                     /locus_tag="ACHOSC_RS13775"
                     /old_locus_tag="pgaptmp_002755"
     CDS             5090..5452
                     /gene="queD"
                     /locus_tag="ACHOSC_RS13775"
                     /old_locus_tag="pgaptmp_002755"
                     /EC_number="4.1.2.50"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708563.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0008616 - queuosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-carboxytetrahydropterin synthase QueD"
                     /protein_id="HGU4548666.1"
                     /translation="MSTTLFKDFTFEAAHRLPHVPEGHKCGRLHGHSFMVRLEITGEV
                     DPHTGWIIDFAELKAAFKPTYERLDHHYLNDIPGLENPTSEVLAKWIWDQVKPVVPLL
                     SAVMVKETCTAGCIYRGE"
     gene            5530..6801
                     /gene="ygcN"
                     /locus_tag="ACHOSC_RS13780"
                     /old_locus_tag="pgaptmp_002756"
     CDS             5530..6801
                     /gene="ygcN"
                     /locus_tag="ACHOSC_RS13780"
                     /old_locus_tag="pgaptmp_002756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417246.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HGU4548667.1"
                     /translation="MEDDCDIIIIGAGIAGTACALRCARAGLSVLLLERAEIPGSKNL
                     SGGRLYTHALAELLPQFHLTAPLERRITHESLSLLTPDGATTFSSLQPGGESWSVLRA
                     RFDPWLVAEAEEEGVECIPGATVDALYEENGRVCGVICGDDILRARYVVLAEGANSVL
                     AERHGLVTRPAGEAMALGIKEVLSLEPSAIEERFHLENNEGAALLFSGRICDDLPGGA
                     FLYTNQQTLSLGIVCPLSSLTQSRVPASELLTRFKAHPAVRPLIKNTESLEYGAHLVP
                     EGGLHSMPVQYAGNGWLLVGDALRSCVNTGISVRGMDMALTGAQAAAQTLISACQHRE
                     PQNLFALYHHNVERSLLWDVLQRYQHVPALLQRPGWYRAWPALMQDISRDLWDQGDKP
                     VPPLRQLFWRHLRRHGLWHLAGDVIRSLRCL"
     gene            6792..7052
                     /gene="ygcO"
                     /locus_tag="ACHOSC_RS13785"
                     /old_locus_tag="pgaptmp_002757"
     CDS             6792..7052
                     /gene="ygcO"
                     /locus_tag="ACHOSC_RS13785"
                     /old_locus_tag="pgaptmp_002757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311649.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin family protein"
                     /protein_id="HGU4548668.1"
                     /translation="MSVAHNLWRVADAPHIVPADSVERQMVQRLINACPAGLFSLTPE
                     GDLRVDYHGCLECGTCCLLCDESTLQQWRYPPSGFGITYRFG"
     gene            7069..7644
                     /gene="ygcP"
                     /locus_tag="ACHOSC_RS13790"
                     /old_locus_tag="pgaptmp_002758"
     CDS             7069..7644
                     /gene="ygcP"
                     /locus_tag="ACHOSC_RS13790"
                     /old_locus_tag="pgaptmp_002758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate responsive antiterminator"
                     /protein_id="HGU4548669.1"
                     /translation="MPLLHLLRQNPVIAAVKDNASLQLAIDSECQFISVLYGNICTIS
                     NIVKKIKNAGKYAFIHVDLLEGASNKEVVIQFLKLVTEADGIISTKASMLKAARAEGF
                     FCIHRLFIVDSISFHNIDKQVAQSNPDCIEILPGCMPKVLGWVTEKIRQPLIAGGLVC
                     DEEDARNAINAGVVALSTTNTGVWTLAKKLL"
     gene            complement(7792..8652)
                     /gene="ygcQ"
                     /locus_tag="ACHOSC_RS13795"
                     /old_locus_tag="pgaptmp_002759"
     CDS             complement(7792..8652)
                     /gene="ygcQ"
                     /locus_tag="ACHOSC_RS13795"
                     /old_locus_tag="pgaptmp_002759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417249.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit
                     alpha/FixB family protein"
                     /protein_id="HGU4548670.1"
                     /translation="MNIAIVTINQENAAIASWLAAQDFSGCTLAHWQIEPQPVVAEQV
                     LDALVEQWQRTPADVVLFPPGTFGDELSTRLAWRLHGASICQVTSLDIPTVSVRKSHW
                     GNALTATLQTEKRPLCLSLARQAGAVKNATLPSGMQQLNIVPGALPDWLVSTEDLKNV
                     TRDPLAEARRVLVVGQGGEADNQEIAMLAEKLGAEVGYSRARVMNGGVDAEKVIGISG
                     HLLAPEVCIVVGASGAAALMAGVRNSKFVVAINHDASAAVFSQADVGVVDDWKVVLEA
                     LVTNIHADCQ"
     gene            complement(8649..9428)
                     /gene="ygcR"
                     /locus_tag="ACHOSC_RS13800"
                     /old_locus_tag="pgaptmp_002760"
     CDS             complement(8649..9428)
                     /gene="ygcR"
                     /locus_tag="ACHOSC_RS13800"
                     /old_locus_tag="pgaptmp_002760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417250.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit beta/FixA
                     family protein"
                     /protein_id="HGU4548671.1"
                     /translation="MNILLAFKAEPDAGMLAEKEWQAAAQGKSGPDISLLRSLLGADE
                     QAAAALLLAQRKNGTPMSLTALSMGDERALHWLRYLMALGFEEAVLLETAADLRFAPE
                     FVARHIAEWQHQNPLDLIITGCQSSEGQNGQTPFLLAEMLGWPCFTQVERFTLDALFI
                     TLEQRIEHGLRCCRVRLPAVIAVRQCGEVALPVPGMRQRMAAGKAEIIRKTVAAEMPA
                     MQCLQLARAEQRRGATLIDGQTVAEKAQKLWQDYLRQRMQP"
     gene            complement(9406..10815)
                     /gene="ygcS"
                     /locus_tag="ACHOSC_RS13805"
                     /old_locus_tag="pgaptmp_002761"
     CDS             complement(9406..10815)
                     /gene="ygcS"
                     /locus_tag="ACHOSC_RS13805"
                     /old_locus_tag="pgaptmp_002761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HGU4548672.1"
                     /translation="MTGRCLFGFSGEKPFLLPDNEGVKMNTSPVRMDDLPLNRFHCRI
                     AALTFGAHLTDGYVLGVIGYAIIQLTPAMQLTPFMAGMIGGSALLGLFLGSLVLGWIS
                     DHIGRQKIFTFSFLLITLASFLQFFATTPEHLIGLRILIGIGLGGDYSVGHTLLAEFS
                     PRRHRGILLGAFSVVWTVGYVLASIAGHHFISENPEAWRWLLASAALPALLITLLRWG
                     TPESPRWLLRQGRFAEAHAIVHRYFGPHVLLGDEVVTATHKHIKTLFSSRYWRRTAFN
                     SVFFVCLVIPWFVIYTWLPTIAQTIGLEDALTASLMLNALLIVGALLGLVLTHLLAHR
                     KFLLGSFLLLAATLVVMACLPSGSSLTLLLFVLFSTTISAVSNLVGILPAESFPTDIR
                     SLGVGFATAMSRLGAAVSTGLLPWVLAQWGMQVTLLLLATVLLVGFVVTWLWAPETKA
                     LPLVAAGNVGGANEHSVSV"
     gene            complement(10837..12291)
                     /gene="ygcU"
                     /locus_tag="ACHOSC_RS13810"
                     /old_locus_tag="pgaptmp_002762"
     CDS             complement(10837..12291)
                     /gene="ygcU"
                     /locus_tag="ACHOSC_RS13810"
                     /old_locus_tag="pgaptmp_002762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HGU4548673.1"
                     /translation="MSLSRAAIVDQLKEIVGADRVITDETVLKKNSIDRFRKFPDIHG
                     IYTLPIPAAVVKLGSTEQVSRVLNFMNAHKINGVPRTGASATEGGLETVVENSVVLDG
                     SAMNQIINIDIENMQATAQCGVPQEVLENALREKGYTTGHSPQSKPLAQMGGLVATRS
                     IGQFSTLYGAIEDMVVGLEAVLADGTVTRIKNVPRRAAGTDIRHIIIGNEGALCYITE
                     VTVKIFKFTPENNLFYGYILEDMKTGFNILREVMVEGYRPSIARLYDAEDGTQHFTHF
                     ADGKCVLIFMAEGNPRIAKATGEGIAEIVARYPQCQRVDSKLIETWFNNLNWGPDKVA
                     AERVQILKTGNMGFTTEVSGCWSCIHEIYESVINRIRTEFPHADDITMLGGHSSHSYQ
                     NGTNMYFVYDYNVVDCKPEEEIDKYHNPLNKIICEETIRLGGSMVHHHGIGKHRVHWS
                     KLEHGSAWALLEGLKKQFDPNGIMNTGTIYPIEK"
     gene            complement(12361..13145)
                     /gene="ygcW"
                     /locus_tag="ACHOSC_RS13815"
                     /old_locus_tag="pgaptmp_002763"
                     /pseudo
     CDS             complement(12361..13145)
                     /gene="ygcW"
                     /locus_tag="ACHOSC_RS13815"
                     /old_locus_tag="pgaptmp_002763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417254.4"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
BASE COUNT         3167 a         3670 c         3397 g         3290 t
ORIGIN      
        1 attcagtacg cggggtttta cccccgcgtg gtggctactt cttcgattcg taagcgagaa
       61 ctgccttaaa ctccatacca cgttctctga tactcaactc acacaacgag tcacctacca
      121 tcagcgtgaa tcccagtact gtacaacaca gtacgatgat tgccacaagg gcatattttg
      181 tcagcatgtc ttgcctccct gcaaagaaga gactaacatt cgaattgttt gggttcgaac
      241 gtaagcctca ggttgataga aatatcgcct ggggcttttg tccatctgga acctcgcgaa
      301 tgcttaacgc cagacagcct caagcacccg acgccattct atacctgata attcttccag
      361 cggcgttcac gtttaaaatc aggaatactg ctcgcaaaat aaaaacgccc ccatcatttc
      421 tgacagaggc gtttaattta tccggcaata tttacccttc gtgcaatcca cattcccttt
      481 ttaagccaaa gaaacgtgtt tcttcttccg acatgccagg ttcccattta cgggttgtat
      541 gggtatcgcc caccgataaa tatccttcgt cccataatgg gtgatatttc aggccatgtt
      601 tttgcagata ctgatatatc gttcggttat cccagtcgat aatcggcagc actttaaata
      661 cgccacgctg aattgccagc accggtaaat tggcgcggct gccggattgt tcgcggcgca
      721 ggccagcaaa ccacgtttgc gcattcagtt ctttcagcgc ccggttcatc ggttcgactt
      781 tgttgatgtc attgtacttt tcaatgcctt caacgccctg ttcccacagt ttgccgtagc
      841 gtgcttcctg ccaggcggcg ctttcggtag cgcggtacac tttcaggttg agcttgagtt
      901 tgtccgttaa ctcgtcaata aagcggtagg tttccgggaa cagatagccg gtgtcggtga
      961 ggatcaccgg aatatccggg tgaatctgat tcaccagatg caggctcacc gccgcctgaa
     1021 tgccaaagct ggaagaaagt acatattcac cgggcagatt atccagcgcc caggcaacgc
     1081 ggccttcagc gtccagtttt tccagttcgg cgttagtttc tgccagcgcc agaatgcgat
     1141 ctaccttcgg cagttcgttc agggcgttta gatcgagttt ggacatagat tcctcactgt
     1201 ttgccttgcc tgatgcgacg ctcacgcgtc ttatcaggcc tacaagaccg ggctgatggt
     1261 taatcccaca aatcgcgcgc cggatcgagc accgggcgaa tgatgcccgc acgcaccgta
     1321 aagtcgccga agccttcacc cgcttcgcgc tctttcgccc agcgccctat cagttcatca
     1381 agcgacgcca ggatttccgg ctcggtgatg ttttctttat acatccgtgg gatacgtgtc
     1441 ccaatgcggt tgccgccaag atgcaggttg tagcgacccg gcgctttacc caccaggccc
     1501 acttccgcca gcatcgcgcg accacaaccg ttcgggcagc ctgttacacg catcacgata
     1561 tgctcatcgc tgacaccatg tttcgccatt aaattatcga tgttgtcgat aaaagacggc
     1621 aggaaacgct ctgcttccgc catcgccagc gggcaagtcg ggaacgagac gcaggccatc
     1681 gagttttcac gctgcggcgt gacggcattc attaacccgc tctctttggc gatcttctcg
     1741 atcttcgctt tctcgctttc cggtacaccg gcgatgatca gattctggtt cgccgtaatg
     1801 cggaaatcgc ctttgtggat cttcgcgatc tccagcaggc cggttttcag cggacgcccc
     1861 ggataatcaa ggatgcgacc attttcgata aacagcgtca ggtgccagtt atcatcaatg
     1921 cccttaaccc agccaatacg atcgcctcgt ccggtgaact catatggacg gatcggttca
     1981 aatttgatcc ccgcgcgacg ctccacttcc gctttaaacg tctcaacccc cacgcgctcc
     2041 agcgtgtatt tggttttggc atttttacga tcggttcggt taccccagtc acgctgagtt
     2101 gtcacgacgg cttcggccac cgccagcgta tgctccagcg gcagatagcc aaactcgctc
     2161 gccgtgcggg cgtaggtttt cttgttgccg tgttcgatag aaagcccacc gcccaccagc
     2221 aggttgaagc ctaccagctt gccgttttcg gcgattgcca cgaagttcat gtcgttggca
     2281 tgcagatcga tatcgttctg cggcgggatc actaccgtgg ttttgaactt acgcggcagg
     2341 taggtctgac caaggatcgg ttcttcatca gtagtggcga ctttttcgtg gtcgagccag
     2401 atctccgcat acgcgcgggt acgaggcaac agatgctcag aaatcttctt cgcccactcg
     2461 tacgcttccg cgtgcagctg cgactcgtaa gggttcgagg tgcagagtac gttacggttc
     2521 atgtcgttag ctgtcgccag cgcatcaaga ccgaccgagt gcagcatctg gtgcaccggt
     2581 ttgacgttct ttttcagaat gccgtggaac tgaaacgtct ggcggttggt caggcgaatg
     2641 ctgccataga tggtgttttc accggcaaat ttgtcgatcg cctgccactg tttagtggta
     2701 atcaccccac ccggcagacg acagcgaagc agcatcgcgt ggcgcggctc cagcttctgt
     2761 tcagcacgtt cggcgcggat gtcgcggtca tcctgctgat acatgccgtg gaagcggatc
     2821 agcaggaagt tgtcgccctt aaagccgccg gtcagaccgt cgtttaaatc ttccgcaatg
     2881 gtgccgcgca ggtagttgct ttcaagcttc atgcgctcgg cgtctgtcag ttttccttcg
     2941 accactaaag gccctggatg tttttcgctc attagtagac atctcgctga taacggcgct
     3001 ctacgcgcag ctcacttaaa aattcatccg ccgcttcggt gtccatgcca ccaaattcgg
     3061 caatcacttc cagaagtgcc tgctcaacgt ctttcgccat gcgattagcg tcgccgcaga
     3121 cataaatgtg ggcaccatca ttgatccagc gccacagctc cgcgccctgt tcgcgcagtt
     3181 tgtcttgtac gtaaactttt tctttttggt cgcgcgacca ggcaagatcg atacgtgtca
     3241 gcacgccatc tttgacgtag cgctgccact ccacctgata caggaagtct tccgtaaagt
     3301 gcggattacc aaagaacagc cagtttttac ctggcgcttc gccggcggcg cgttgctgca
     3361 taaaggcgcg gaacggcgca ataccggtgc ctgggccaat cataatcacc ggggtttccg
     3421 ggttagcggg caggcgaaag ttatcgttat gttcgataaa tacgcggact tcgccctctt
     3481 cttccacgcg atccgcgagg aagctggagg caccaccggc acgggctcgg ccttccacgt
     3541 cgtaacgcac cacaccaacg gtgacgtgta cttcgttctc gacttccgcc tgcgaggagg
     3601 cgatggaata caggcgcggg gtcagcgggc gcagcagatt aatcagcgct tcggcatcca
     3661 gttgcgccgg agagaaacgc accatgtcga caatcggcat cgtcgcggcg taatgctgta
     3721 acttcgcttt atcgcccacc agcggcagca gtgtttcgct gcgggtaagc gtggcgtaat
     3781 tctcaacaat gttggcggtg ttgacggtca gttcgaagtg ccactgtagc gcttcgttca
     3841 gaggcaacgt tttgccctcg acggtgacag gttcatcgcc tttcagccac agcagttcga
     3901 caagttcttt caccagtgcc ggatcgttct gataccagac gcccagcgcg tcacccggct
     3961 ggtaacgcag gcccgagtca cctaagtcaa tttcgatatg gcgaacgtct ttttcagagt
     4021 tacgcccggt aattttctgg ttaacagaga ggctagccac cagcggcgcg tctttgctgt
     4081 acgggctggt gtggatttca tttaccgcgc cagtagcgac ggattgcgaa ggtgccgcga
     4141 caggcgcacg cgatttaagc gcatcaacca cgcgggcgcg ccactcgctg gcagcagcct
     4201 ggtattcaac atcggcatcg acacggtcga gcaggcgttc accacccagt tccgccagct
     4261 tgctgtcgaa atctttcccg gactggcaga aaaattcata agagctatcg ccgaggctaa
     4321 acacggcaaa cgcggtgttt tccagctttg gcgctttttt ggagaacagg aacttatgca
     4381 gcgcgacggc ttcttccggc ggttcccctt ccccttgcgt tgacgtcact acgatgagca
     4441 gtttttcgct ggcgatttgt ttgaatttat agtcgcccgc gttcaccagc ttaacgttca
     4501 gttttgctgc taataaatca tcgcgtaatg cttcagcaac ccggcgcgca ttgccggttt
     4561 gcgaggcgga gataatagtt atacccggca tttctgcggc tggcgctggc gtcgctgcaa
     4621 gcgcagcagg ctgctgattg agcacgcccc agaaatagcc agaaacccag gcaagctggg
     4681 tgggagttaa atcggtcgtg gccgcctgaa ggcgtgccag ttgctccggg ttcaacggaa
     4741 gcaacgcgga aagtgggacc tgtgtcgtca tgcgtcgtta tgttccagta agcaaagctg
     4801 tttctgcgcc ctgtcagcgc ccataaaaca gaagagaagg taaggttaac ggggtgaacg
     4861 gtgtggatta aagacgggat agcgataact aataaccaaa tcgactaacc tgttttagca
     4921 atagtcttta acaacaaaat agattaacca acctaatgaa aaacaaatga atttagccaa
     4981 tcattaagat aaatcagcga ttttgcgcaa caaagtcgtt ttagataatg cgaaaaaaca
     5041 gcctttccgg tactctacgg cggttttatc gtcctgtaga gaaattatga tgtccaccac
     5101 gttatttaaa gatttcacct tcgaagccgc tcaccgctta ccacacgtcc cggaagggca
     5161 taaatgtggt cgcctgcacg ggcattcctt tatggtgcga ctggaaatta ccggggaagt
     5221 cgatccgcat acgggctgga ttatcgattt cgctgaacta aaagcggcgt ttaaaccaac
     5281 ctacgagcgc ctcgatcacc attatctcaa tgatattcca ggtctggaaa acccaaccag
     5341 cgaggtttta gcaaaatgga tttgggatca ggttaaaccc gttgtgccgc tgttaagtgc
     5401 ggtgatggta aaagaaacct gtaccgcagg ttgtatctat cgcggcgaat gataagagtg
     5461 tgtcggcggt caatttccct taagtaacgc tatgttaggg tgttgtgttc tggatatctg
     5521 gggcatgaca tggaagacga ctgcgacatt attattattg gtgccggtat tgcaggcacc
     5581 gcttgcgcgt tacgctgcgc gcgagcgggt ttatccgttt tgttactgga acgcgctgaa
     5641 atccccggca gcaaaaatct ttccggcggg cggttatata cccatgcact cgcggaactc
     5701 ctcccgcaat ttcatctgac cgcgcctctt gaacgacgca tcactcacga aagcctttcc
     5761 ctgttaacgc cggatggcgc aacgacgttt tccagcttac agcccggcgg tgaatcctgg
     5821 agtgtattac gtgcacggtt cgatccgtgg ctggttgccg aagccgaaga agaaggtgtc
     5881 gaatgcatcc ccggagcgac ggtggatgca ctgtatgaag aaaacggcag ggtgtgtggt
     5941 gtcatttgtg gtgacgatat tctccgtgcc cgttatgtgg tgctggcaga aggtgccaac
     6001 agcgtcctgg ctgagcgtca cgggttagtg actcgtcctg ctggtgaagc gatggcgttg
     6061 gggatcaaag aagtgctgtc gctggaacca tccgctattg aagaacgttt tcatctggag
     6121 aataacgaag gcgcagcgtt gctgttcagc ggcaggatct gtgatgactt acccggcggc
     6181 gcatttcttt atactaatca acaaacgctc tcgttaggga ttgtttgccc gctctcttcc
     6241 cttacgcaaa gtcgtgttcc ggcaagcgag ctgctgactc gctttaaagc gcatccggca
     6301 gtgcgcccgc ttatcaaaaa cacggaatca ctggagtatg gtgcgcatct ggtgccagaa
     6361 ggtggcttgc acagtatgcc ggtgcaatac gccggtaacg gctggctgct ggtgggcgat
     6421 gcgttgcgca gttgcgtcaa taccggaatt tccgtgcgcg gcatggatat ggcgctgact
     6481 ggcgcgcagg cggcggcaca aacgctgata agcgcctgcc agcaccgcga gccgcaaaat
     6541 ctgtttgcgc tttatcatca caacgtagag cgcagcctgc tatgggatgt tctacaacgt
     6601 tatcagcatg ttccggcgct tttgcaacgc cctggctggt atcgggcgtg gcctgcgtta
     6661 atgcaggata tttcccgcga tttatgggat cagggtgata aacctgttcc accgctgcgc
     6721 cagttattct ggcgtcattt acgtcgtcat ggcctgtggc atctggcggg cgatgttatc
     6781 aggagtctgc gatgtctgta gcccataatc tctggcgcgt tgctgatgcg ccgcacattg
     6841 ttccggctga ctccgttgag cgtcagatgg tacaacggtt gattaacgcc tgtccggcag
     6901 gtcttttttc gctcacaccg gaaggtgact tacgcgttga ttatcacggc tgcctggagt
     6961 gtggcacctg ctgtttgctg tgcgacgaat ccacactaca acagtggcgc tatccgcctt
     7021 ccggattcgg catcacctac cgctttggat aaataataag gataatttat gcccctctta
     7081 cacctgctcc gccagaatcc ggtgattgct gccgttaaag ataatgccag cctgcaactg
     7141 gcaatcgact ctgaatgcca gtttatttcc gtgttgtacg gcaatatctg caccatcagt
     7201 aatatcgtta aaaagattaa gaacgccggg aaatatgctt ttattcatgt tgatttgctg
     7261 gaaggcgcgt caaataaaga agtcgtgatt cagtttttga aactggtgac cgaggcggac
     7321 ggcattatca gtaccaaagc ctcaatgttg aaagcggcaa gagcggaagg ttttttctgt
     7381 attcatcgcc tgtttattgt tgattcaatt tcgtttcaca acattgataa gcaagttgcg
     7441 caatcgaatc cggattgtat tgagatcctg ccaggctgta tgcccaaagt gctgggctgg
     7501 gtgacagaga aaatccgcca accgctgatt gccggtgggc tggtgtgcga tgaagaagat
     7561 gcgcgtaatg cgattaacgc gggtgtcgtg gcgctttcca ccacgaatac cggggtctgg
     7621 acgttagcga aaaaattact ttgacgggat aatcgtaacc aattgaattt ggtttgattt
     7681 gtaggccgca cgccacatcc gacattcagc gcctgatgcg acgcttgacg cgtcttatca
     7741 ggcctacaag tccccaggcc agaaccgtag gcaggatcat ccggcaacac gttattggca
     7801 atctgcgtga atgtttgtca ccagtgcctc cagcaccact ttccagtcat caaccacgcc
     7861 aacatctgcc tgtgaaaata ccgcagcgct ggcgtcatga ttaatcgcca ccacaaactt
     7921 gctattgcgt acgcccgcca ttaacgctgc tgcgccagaa gcgccaacaa caatacacac
     7981 ctcaggtgcc agcagatgtc cggaaatacc aatcactttt tcagcatcaa cgccaccgtt
     8041 catcactcgc gcccgactgt agcccacttc ggctcccagc ttttctgcca gcatagcgat
     8101 ttcctggtta tctgcctcac cgccctgccc caccaccagc acccgtctgg cctcagctaa
     8161 tgggtcacgg gtaacatttt tcagatcctc tgtgctaacc agccagtcgg gaagcgcgcc
     8221 ggggacaatg ttcagttgct gcataccaga gggcaacgtg gcgtttttca ctgctcccgc
     8281 ctgacgcgcc agggaaaggc atagcgggcg tttttctgtt tgcagcgtcg ccgttagcgc
     8341 atttccccag tgggatttgc gcacgcttac ggttggaata tctaacgaag tcacctggca
     8401 gatgcttgca ccatgtaaac gccaggccag ccgggtcgac agttcatcgc caaacgttcc
     8461 cggcggaaag agaacaacgt ccgcaggcgt tcgctgccac tgttccacca acgcatccag
     8521 cacctgttcc gccactacag gctgaggttc aatctgccag tgcgccaggg tacaaccgct
     8581 gaaatcctgc gccgccagcc agcttgctat tgcggcattc tcctgattta ttgtcacaat
     8641 tgcgatattc atggctgcat cctttggcgt aaatagtcct gccatagctt ttgagctttt
     8701 tctgctaccg tttgcccatc aatcagcgtc gctccgcgtc gttgctctgc tcttgccagt
     8761 tgcagacact gcatagctgg catttcggca gcaacagttt tacggataat ttctgctttc
     8821 cccgcagcca ttcgctggcg cattcccggt actggcaagg ccacttcacc gcactgacgc
     8881 acggcaatca ccgcaggcaa gcgaacccgg caacaacgca aaccatgttc aatacgctgt
     8941 tcgagggtga taaacagcgc gtcgagagtg aaacgctcca cctgggtgaa gcagggccag
     9001 cccagcattt ccgccagcaa aaatggcgtt tgcccattct gcccttcgct gctttggcag
     9061 ccggtaatta tcagatccag cggattctga tgctgccatt cggcaatatg gcgagcgaca
     9121 aattccggcg caaagcgcag atctgccgcc gtctccagca atacagcttc ctcaaaccca
     9181 agagccatga gatagcgcag ccagtgcaat gcccgttcat cccccatgct caacgcggtt
     9241 aaagacatcg gcgtaccgtt ttttctctgc gccaacagca gcgcggcagc ggcctgttca
     9301 tcagcaccga gtaaacttcg cagtagcgaa atatccggtc cgcttttacc ctgagccgcc
     9361 gcctgccact ctttttccgc cagcattccg gcatccggtt cggctttaaa cgctaacaga
     9421 atgttcattc gcacctccta catttcccgc cgccaccagc gggagggctt tagtttctgg
     9481 tgcccatagc caggtcacaa caaaaccaac caacaacact gtcgccagga gcaataaggt
     9541 gacttgcatt ccccactgcg ccagcaccca cggcagcagg ccagtactta ccgccgcgcc
     9601 aagtcgactc atggcggtgg caaaaccgac gcccagcgag cgaatgtcag taggaaaact
     9661 ttccgcaggc aaaatgccca ccagattact gactgccgaa atggtggtgc tgaagagaac
     9721 aaaaagcagc agcgttaatg aactgccgga aggcaaacag gccattacta ccagcgttgc
     9781 cgccagcagc aaaaaacttc ccagcaaaaa tttgcgatgt gccagcaggt gcgtcagaac
     9841 taatcccagc agcgcgccca caattaacaa cgcattaagc atcaggctgg cagtcagcgc
     9901 atcttccaga ccaatagtct gggcgatagt tggcagccag gtataaatca caaaccatgg
     9961 gattacgagg cagacaaaga agacgctgtt aaacgccgtg cgccgccagt aacgcgaaga
    10021 gaacaaggtt ttgatgtgtt tatgggtcgc cgttaccact tcatcgccca gtaaaacatg
    10081 gggaccaaaa tagcgatgca cgatagcgtg agcttctgca aaacgcccct ggcgcagtag
    10141 ccagcgtggt gattctggcg ttccccagcg taataacgta atcaacaacg cgggcagagc
    10201 tgccgatgcc agtagccagc gccaggcctc cgggttttcg gaaataaagt gatgtccggc
    10261 aatacttgcc agcacatagc ctacggtcca caccacgctg aatgcgccca gcaaaatacc
    10321 gcgatggcgg cgcggggaaa attcagccag caaggtgtga cctactgaat aatcgcctcc
    10381 cagaccaatg ccaatcaaaa tgcgcagtcc aataagatgc tctggcgtgg tggcaaaaaa
    10441 ttgtaaaaac gaagcaagcg taatcagcaa aaagctgaag gtgaagattt tttgccgacc
    10501 aatatggtcg gagatccacc caagaaccag gctgccaagg aacaaaccaa ggagcgccga
    10561 gccgccgatc attcccgcca taaacggcgt cagttgcatg gcgggcgtaa gctgaataat
    10621 ggcgtaacca atgacgccga gaacataacc gtcggtcagg tgtgcgccga aagtgagcgc
    10681 agcaatgcgg cagtgaaaac ggttaagcgg taaatcatcc attcgcaccg gtgaagtgtt
    10741 catctttacc ccttcattgt ccggtaataa gaacggcttc tcgcctgaga agccgaaaag
    10801 gcaacgcccc gtcatcgcgg aacgctgcct gacacattat ttttcaatcg gatagatagt
    10861 acccgtattc ataatgccat taggatcgaa ctgctttttc agcccttcca gcaacgccca
    10921 cgcgctgccg tgttccagtt tgctccagtg aacgcgatgt ttaccgatac cgtggtggtg
    10981 caccatcgaa ccgccgaggc gaatggtttc ttcacagatg atcttgttga gcggattgtg
    11041 gtacttgtcg atttcctctt ccggcttaca gtcaacgacg ttgtaatcgt agacgaagta
    11101 catgttggtg ccgttctgat agctatgaga ggaatgaccg cccagcatgg tgatgtcgtc
    11161 ggcgtgcggg aactcagtac gaatacggtt aataacgctt tcgtagattt cgtggatgca
    11221 gctccagcag ccggacactt cagtggtaaa gcccatgttg ccggttttga ggatctgcac
    11281 acgttcggca gccactttat ccggtcccca gttcaggttg ttaaaccagg tttcgatcag
    11341 tttgctgtcc acgcgctggc actgcgggta gcgggcaacg atttccgcaa tcccttcgcc
    11401 cgtcgccttc gcaatgcgag ggttaccttc agccataaag atcagcacac attttccgtc
    11461 tgcaaaatgg gtgaagtgtt gggtgccatc ttcagcgtca tacaaacgag cgatcgacgg
    11521 acgataccct tccaccatga cttcacgcag gatgttgaag ccggttttca tgtcttccag
    11581 gatatagccg tagaagaggt tgttttccgg ggtgaattta aagattttca ctgttacttc
    11641 agtgatatag caaagtgcac cttcgttacc gatgatgatg tggcgaatgt ccgtgccagc
    11701 cgcgcggcgt ggcacgtttt taatgcgtgt gacggtgcca tcagccaata ctgcttccag
    11761 accaacgacc atatcttcga ttgcgccgta gagtgtggag aactgcccga tactgcgggt
    11821 tgctaccagg ccgcccatct gcgccagcgg ctttgactgc ggagaatgcc ccgtggtgta
    11881 acctttttca cgcaacgcat tttccagcac ttcctgggga acgccacatt gcgccgtcgc
    11941 ctgcatattc tcaatatcaa tattaatgat ttgattcatg gcggaaccgt cgagcaccac
    12001 cgagttttct acaacagttt ccagcccacc ttcagtggcg gaagcgccgg tacgcggcac
    12061 accgttaatt ttgtgcgcat tcataaaatt cagcacacgg gagacttgtt ctgtggaacc
    12121 gagttttacg accgctgccg gaatcggcaa agtataaata ccatgaatat ccggaaattt
    12181 acgaaaacgg tcaatactgt tcttctttaa tactgtttca tcggtaatta cgcgatctgc
    12241 accaacaatt tcctttagtt ggtcgacaat cgctgcgcga gataaagaca tagtaattcc
    12301 ttcctgataa taaaaatgga ataatcatta ataagagaaa acagaaacca ccgttatgga
    12361 ttagcgcact aaataaccac catcgaccac taataaatgc ccattgacat aatttgaagc
    12421 cggacttgcg aggaatacag ctgcgcccat taaatcctga gtatcgcccc aacggtttgc
    12481 cggaattgat caagaacgcg ctgattggtt tctggattac tgcgtgtcgc cagcgtaata
    12541 tctgttgcat aatagccagg ggcaatacca tttacctgaa tattatattg acctagttca
    12601 tcacaataag ctttggtgaa tccggcaaga gcatgtttag tggcagaata tgcaggtgac
    12661 cattgtccac ctaagtaaga gaacaatgaa cagatattaa taattttgcc gcttttctgc
    12721 gggatcataa tttttgcagc ttcatagctt aactcgaatg cggcggtcag gttcacatca
    12781 atcatcggat cccagtcggc acgaccgaag tccagcacct tattcagctt acaaataccg
    12841 gcattgttta ccagaatatc aactgtaccg aaacgctcac agcaagcagc gataatcttc
    12901 tgcggcgcgc cttctgcggt gatatccacc tgcatgaagt ccacctcaac accctgtttt
    12961 tcaatcattt cctttgtttc gccgttatct ttgacgaaac taggaataaa gatatttgcg
    13021 ccagctttgg ccaacgccat ggcaaatgcc tagcctaaac cgctattccc accggtaaca
    13081 attgcggttt tacctttcag tgagaaaaaa tccattgaga acgcattgag agattcgatt
    13141 gacatagtta gctccaattt ccgtaaggca aaaaaaagaa aggtaacttc ccccgcgaga
    13201 gggaagttac ctttctcatt atctctggta actggagata atttttagca taacggcata
    13261 actgctaatg tgatcgtaat cacagtgtga tattcgtatt gtcaaatagt tttaaaaagc
    13321 agaagcgtga ttactctcac acaacacctc tgctttccat gttatttctt attgcaatta
    13381 ctagagacct gagaaaagta atccacctga aagggcggct tacttttctc ttttttttgt
    13441 ctattacaaa tgggaatacg atatgcaaca caactcatat cgccgttgga taaccctcgc
    13501 gataattagt tttagcggcg gcgt
//
LOCUS       DAZKHF020000117        13346 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2201S31241
            SAMN43267800-rid25106413.denovo.118, whole genome shotgun sequence.
ACCESSION   DAZKHF020000117 DAZKHF020000000
VERSION     DAZKHF020000117.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267800
            Sequence Read Archive: SRR30307263
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 13346)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13346)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 13346)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350581.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350581.2
            Genome Coverage       :: 151x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:21:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,652
            CDSs (total)                      :: 5,557
            Genes (coding)                    :: 5,320
            CDSs (with protein)               :: 5,320
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 237
            CDSs (without protein)            :: 237
            Pseudo Genes (ambiguous residues) :: 0 of 237
            Pseudo Genes (frameshifted)       :: 102 of 237
            Pseudo Genes (incomplete)         :: 130 of 237
            Pseudo Genes (internal stop)      :: 52 of 237
            Pseudo Genes (multiple problems)  :: 41 of 237
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13346
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267800-rid25106413.denovo.118"
                     /serotype="O 26:H11"
                     /isolate="2201S31241"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-01"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            38..394
                     /locus_tag="ACHOSC_RS13820"
                     /old_locus_tag="pgaptmp_002764"
     CDS             38..394
                     /locus_tag="ACHOSC_RS13820"
                     /old_locus_tag="pgaptmp_002764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF805 domain-containing protein"
                     /protein_id="HGU4548674.1"
                     /translation="MQWYLAVLKNYVGFSGRARRKEYWMFTLINAIVGAIINVIQLIL
                     GLEFPFLSLIYLAATIIPVIALCVRRLHDTDRSGAWALLYLVPIIGWLVLFVFACLEG
                     NSGSNRYGNDPKFGSN"
     gene            complement(445..1341)
                     /gene="yhaJ"
                     /locus_tag="ACHOSC_RS13825"
                     /old_locus_tag="pgaptmp_002765"
     CDS             complement(445..1341)
                     /gene="yhaJ"
                     /locus_tag="ACHOSC_RS13825"
                     /old_locus_tag="pgaptmp_002765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator YhaJ"
                     /protein_id="HGU4548675.1"
                     /translation="MAKERALTLEALRVMDAIDRRGSFAAAADELGRVPSALSYTMQK
                     LEEELDVVLFDRSGHRTKFTNVGRMLLERGRVLLEAADKLTTDAEALARGWETHLTIV
                     TEALVPTPAFFPLIDKLAAKANTQLAIITEVLAGAWERLEQGRADIVIAPDMHFRSSS
                     EINSRKLYTLMNVYVAAPDHPIHQEPEPLSEVTRVKYRGIAVADTARERPVLTVQLLD
                     KQPRLTVSTIEDKRQALLAGLGVATMPYPMVEKDIAEGRLRVVSPESTSEIDIIMAWR
                     RDSMGEAKSWCLREIPKLFSGK"
     gene            1446..2147
                     /locus_tag="ACHOSC_RS13830"
                     /old_locus_tag="pgaptmp_002766"
     CDS             1446..2147
                     /locus_tag="ACHOSC_RS13830"
                     /old_locus_tag="pgaptmp_002766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pirin family protein"
                     /protein_id="HGU4548676.1"
                     /translation="MITTRTARQCGQADYGWLQARYTFSFGHYFDPKLLGYASLRVLN
                     QEVLAPDAAFQPRTYPKVDILNVILDGEAEYRDSEGNHVQASAGEALLLSTQPGVSYS
                     EHNLSKDKPLTRMQLWLDACPQRENPLIQKLALNMGKQQLIASPEGTMGSLQLRQQVW
                     LHHIVLDKGESANFQLHGPRAYLQSIHGKFHALTHHEEKAALTCGDGAFIRDEANITL
                     VADSPLRALLIDLPV"
     gene            2170..2334
                     /gene="yhaL"
                     /locus_tag="ACHOSC_RS13835"
                     /old_locus_tag="pgaptmp_002767"
     CDS             2170..2334
                     /gene="yhaL"
                     /locus_tag="ACHOSC_RS13835"
                     /old_locus_tag="pgaptmp_002767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312016.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YhaL"
                     /protein_id="HGU4548677.1"
                     /translation="MSKKSAKKRQPVKPVVAKEPARTAKNFGYEEMLSELEAIVADAE
                     TRLAEDEATA"
     gene            complement(2546..3856)
                     /gene="cyuA"
                     /locus_tag="ACHOSC_RS13840"
                     /old_locus_tag="pgaptmp_002768"
     CDS             complement(2546..3856)
                     /gene="cyuA"
                     /locus_tag="ACHOSC_RS13840"
                     /old_locus_tag="pgaptmp_002768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfidase"
                     /protein_id="HGU4548678.1"
                     /translation="MFDSTLNPLWQRYILAVQEEVKPALGCTEPISLALAAAVAAAEL
                     EGPVERVEAWVSPNLMKNGLGVTVPGTGMVGLPIAAALGALGGNANAGLEVLKDATAQ
                     AIADAKALLAAGKVSVKIQEPCNEILFSRAKVWNGEKWACVTIVGGHTNIVHIETHNG
                     VVFTQQACVAEGEQESPLTVLSRTTLAEILKFVNEVPFAAIRFILDSAKLNCALSQEG
                     LSGKWGLHIGATLEKQCERGLLAKDLSSSIVIRTSAASDARMGGATLPAMSNSGSGNQ
                     GITATMPVVVVAEHFGADDERLARALMLSHLSAIYIHNQLPRLSALCAATTAAMGAAA
                     GMAWLVDGRYETISMAISSMIGDVSGMICDGASNSCAMKVSTSASAAWKAVLMALDDT
                     AVTGNEGIVAHDVEQSIANLCALASHSMQQTDRQIIEIMASKAR"
     gene            complement(3884..5215)
                     /locus_tag="ACHOSC_RS13845"
                     /old_locus_tag="pgaptmp_002769"
     CDS             complement(3884..5215)
                     /locus_tag="ACHOSC_RS13845"
                     /old_locus_tag="pgaptmp_002769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026203.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HGU4548679.1"
                     /translation="MEIASNKGVIADASTPAGRAGMSESEWREAIKFDSTDTGWVIMS
                     IGMAIGAGIVFLPVQVGLMGLWVFLLSSVIGYPAMYLFQRLFINTLAESPECKDYPSV
                     ISGYLGKNWGILLGALYFVMLVIWMFVYSTAITNDSASYLHTFGVTEGLLSDSPFYGL
                     VLICILVAISSRGEKLLFKISTGMVLTKLLVVAALGVSMVGMWHLYNVGSLPPLGLLV
                     KNAIITLPFTLTSILFIQTLSPMVISYRSREKSIEVARHKALRAMNIAFGILFVTVFF
                     YAVSFTLAMGHDEAVKAYEQNISALAIAAQFISGDGAAWVKVVSVILNIFAVMTAFFG
                     VYLGFREATQGIVMNILRRKMPAEKINENLVQRGIMIFAILLAWSAIVLNAPVLSFTS
                     ICSPIFGMVGCLIPAWLVYKVPALHKYKGMSLYLIIVTGLLLCVSPFLAFS"
     gene            complement(5490..6854)
                     /gene="tdcG"
                     /locus_tag="ACHOSC_RS13850"
                     /old_locus_tag="pgaptmp_002770"
     CDS             complement(5490..6854)
                     /gene="tdcG"
                     /locus_tag="ACHOSC_RS13850"
                     /old_locus_tag="pgaptmp_002770"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase"
                     /protein_id="HGU4548680.1"
                     /translation="MISAFDIFKIGIGPSSSHTVGPMNAGKSFIDRLESSGLLTATSH
                     IVVDLYGSLSLTGKGHATDVAIIMGLAGNSPQDVVIDEIPAFIELVTRSGRLPVASGA
                     HIVDFPVAKNIIFHPEMLPRHENGMRITAWKGQEALLSKTYYSVGGGFIVEEEHFGLS
                     HDVETSVPYDFHSAGELLKMCDYNGLSISGLMMHNELALRSKAEIDAGFARIWQVMHD
                     GIERGMNTEGVLPGPLNVPRRAVALRRQLVSSDNISNDPMNVIDWINMYALAVSEENA
                     AGGRVVTAPTNGACGIIPAVLAYYDKFRRPVNERSIARYFLAAGAIGALYKMNASISG
                     AEVGCQGEIGVACSMAAAGLTELLGGSPAQVCNAAEIAMEHNLGLTCDPVAGQVQIPC
                     IERNAINAVKAVNAARMAMRRTSAPRVSLDKVIETMYETGKDMNDKYRETSRGGLAIK
                     VVCG"
     gene            complement(6926..7315)
                     /locus_tag="ACHOSC_RS13855"
                     /old_locus_tag="pgaptmp_002771"
     CDS             complement(6926..7315)
                     /locus_tag="ACHOSC_RS13855"
                     /old_locus_tag="pgaptmp_002771"
                     /EC_number="3.5.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312020.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enamine/imine deaminase"
                     /protein_id="HGU4548681.1"
                     /translation="MKKIIETQRAPGAIGPYVQGVDLGSMVFTSGQIPVCPQTGEIPA
                     DVQDQARLSLENVKAIVVAAGLSVGDIIKMTVFITDLNDFATINEVYKQFFDEHQATY
                     PTRSCVQVARLPKDVKLEIEAIAVRSA"
     gene            complement(7329..9623)
                     /gene="tdcE"
                     /locus_tag="ACHOSC_RS13860"
                     /old_locus_tag="pgaptmp_002772"
     CDS             complement(7329..9623)
                     /gene="tdcE"
                     /locus_tag="ACHOSC_RS13860"
                     /old_locus_tag="pgaptmp_002772"
                     /EC_number="2.3.1.-"
                     /EC_number="2.3.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-ketobutyrate formate-lyase/pyruvate
                     formate-lyase"
                     /protein_id="HGU4548682.1"
                     /translation="MKVDIDTSDKLYADAWLGFKGTDWKNEINVRDFIQHNYTPYEGD
                     ESFLAEATPATTELWEKVMEGIRIENATHAPVDFDTNIATTITAHDAGYINQPLEKIV
                     GLQTDAPLKRALHPFGGINMIKSSFHAYGREMDSEFEYLFTDLRKTHNQGVFDVYSPD
                     MLRCRKSGVLTGLPDGYGRGRIIGDYRRVALYGISYLVRERELQFADLQSRLEKGEDL
                     EATIRLREELAEHRHALLQIQEMAAKYGFDISRPAQNAQEAVQWLYFAYLAAVKSQNG
                     GAMSLGRTASFLDIYIERDFKAGVLNEQQAQELIDHFIMKIRMVRFLRTPEFDSLFSG
                     DPIWATEVIGGMGLDGRTLVTKNSFRYLHTLHTMGPAPEPNLTILWSEELPIAFKKYA
                     AQVSIVTSSLQYENDDLMRTDFNSDDYAIACCVSPMVIGKQMQFFGARANLAKTLLYA
                     INGGVDEKLKIQVGPKTAPLMDDVLDYDKVMDSLDHFMDWLAVQYISALNIIHYMHDK
                     YSYEASLMALHDRDVYRTMACGIAGLSVATDSLSAIKYARVKPIRDENGLAVDFEIDG
                     EYPQYGNNDERVDSIACDLVERFMKKIKALPTYRNAVTTQSILTITSNVVYGQKTGNT
                     PDGRRAGTPFAPGANPMHGRDRKGAVASLTSVAKLPFTYAKDGISYTFSIVPAALGKE
                     DPVRKTNLVGLLDGYFHHEADVEGGQHLNVNVMNREMLLDAIEHPEKYPNLTIRVSGY
                     AVRFNALTREQQQDVISRTFTQAL"
     gene            complement(9657..10865)
                     /gene="tdcD"
                     /locus_tag="ACHOSC_RS13865"
                     /old_locus_tag="pgaptmp_002773"
     CDS             complement(9657..10865)
                     /gene="tdcD"
                     /locus_tag="ACHOSC_RS13865"
                     /old_locus_tag="pgaptmp_002773"
                     /EC_number="2.7.2.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417585.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0006082 - organic acid metabolic process
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="propionate kinase"
                     /protein_id="HGU4548683.1"
                     /translation="MNEFPVVLVINCGSSSIKFSVLDASDCEVLMSGIADGINSENAF
                     LSVNGGEPAPLAHHSYEGALKAIAFELEKRNLNDSVALIGHRIAHGGSIFTESAIITD
                     EVIDNIRRVSPLAPLHNYANLSGIESAQQLFPGVTQVAVFDTSFHQTMAPEAYLYGLP
                     WKYYEELGVRRYGFHGTSHRYVSQRAHSLLNLAEDDSGLVVAHLGNGASICAVRNGQS
                     VDTSMGMTPLEGLMMGTRSGDVDFGAMSWVASQTNQSLGDLERVVNKESGLLGISGLS
                     SDLRVLEKAWHEGHERAQLAIKTFVHRIARHIAGHAASLRRLDGIIFTGGIGENSSLI
                     RRLVMEHLAVLGVEIDTEMNNRSNSCGERIVSSENARVICAVIPTNEEKMIALDAIHL
                     GKVNAPAEFA"
     gene            complement(10891..12222)
                     /gene="tdcC"
                     /locus_tag="ACHOSC_RS13870"
                     /old_locus_tag="pgaptmp_002774"
     CDS             complement(10891..12222)
                     /gene="tdcC"
                     /locus_tag="ACHOSC_RS13870"
                     /old_locus_tag="pgaptmp_002774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine transporter TdcC"
                     /protein_id="HGU4548684.1"
                     /translation="MSTSDSIVSSQTKQSSWRKSDTTWTLGLFGTAIGAGVLFFPIRA
                     GFGGLIPILLMLVLAYPIAFYCHRALARLCLSGSNPSGNITETVEEHFGKTGGVVITF
                     LYFFAICPLLWIYGVTITNTFMTFWENQLGFAPLNRGFVALFLLLLMAFVIWFGKDLM
                     VKVMSYLVWPFIASLVLISLSLIPYWNSAVIDQVDLGSLSLTGHDGILITVWLGISIM
                     VFSFNFSPIVSSFVVSKREEYEKDFGRDFTERKCSQIISRASMLMVAVVMFFAFSCLF
                     TLSPANMAEAKAQNIPVLSYLANHFASMTGTKTTFAITLEYAASIIALVAIFKSFFGH
                     YLGTLEGLNGLILKFGYKGDKTKVSLGKLNTISMIFIMGSTWVVAYANPNILDLIEAM
                     GAPIIASLLCLLPMYAIRKAPSLAKYRGRLDNVFVTVIGLLTILNIVYKLF"
     gene            complement(12244..13233)
                     /gene="tdcB"
                     /locus_tag="ACHOSC_RS13875"
                     /old_locus_tag="pgaptmp_002775"
     CDS             complement(12244..13233)
                     /gene="tdcB"
                     /locus_tag="ACHOSC_RS13875"
                     /old_locus_tag="pgaptmp_002775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004794 - threonine deaminase activity
                     [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0006567 - threonine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional threonine ammonia-lyase/L-serine
                     ammonia-lyase TdcB"
                     /protein_id="HGU4548685.1"
                     /translation="MHITYDLPVAIDDIIEAKQRLAGRIYKTGMPRSNYFSERCKGEI
                     FLKFENMQRTGSFKIRGAFNKLSSLTDAEKRKGVVACSAGNHAQGVSLSCAMLGIDGK
                     VVMPKGAPKSKVAATCDYSAEVVLHGDNFNDTIAKVSEIVEMEGRIFIPPYDDPKVIA
                     GQGTIGLEIMEDLYDVDNVIVPIGGGGLIAGIAVAIKSINPTIRVIGVQSENVHGMAA
                     SFHSGEITTHRTTGTLADGCDVSRPGNLTYEIVRELVDDIVLVSEDEIRNSMIALIQR
                     NKVVTEGAGALACAALLSGKLDQYIQNRKTVSIISGGNIDLSRVSQITGFVDA"
BASE COUNT         3404 a         3625 c         3169 g         3148 t
ORIGIN      
        1 atagactttc gtcactttta attaaaggga tgtttttatg cagtggtatt tagccgtatt
       61 aaaaaattat gttggtttct ctggtcgcgc acgtcgtaaa gagtactgga tgtttactct
      121 gattaacgcc atcgtcggcg ctattatcaa tgtcattcaa ttgattttag gtttagagtt
      181 tccattcctt tctctcattt acctggcagc aacaatcatt ccggtcattg ctctgtgtgt
      241 tcgtcgtctg cacgatactg accgttcagg tgcgtgggcg cttctctatc tggtaccgat
      301 cattggctgg ttagttctgt ttgtatttgc ctgtttagaa ggcaattctg gcagcaatcg
      361 ttacggaaac gatcctaagt ttggttcaaa ttaatcttag aattggggcg acatttcgcc
      421 cctttttatt aataataaca aatattattt tccgctaaaa agtttgggaa tttcccgcag
      481 acaccaggat ttcgcttccc ccatactgtc acgacgccag gccataataa tatcgatctc
      541 gctggtcgat tccgggctga cgacacgcaa ccgcccttcc gcaatatctt tttcgaccat
      601 cggatacggc atcgtcgcca cgccaagccc cgccagtaat gcctgacgtt tatcttcaat
      661 cgtgctcacc gttaagcgcg gctgtttgtc cagcagctgt acggtcaaca ccgggcgctc
      721 acgagcggta tccgctaccg caattccacg atatttcacg cgcgtcactt cagataacgg
      781 ttccggctcc tgatgaatcg ggtgatcagg cgcggcaacg tagacgttca ttaacgtata
      841 gagcttgcgc gagttgatct ccgacgagga acgaaaatgc atatccggcg cgataacaat
      901 atccgcccgc ccctgctcca gtcgttccca cgcccccgcc agcacttcgg tgatgattgc
      961 cagttgggta ttggcttttg ccgccagttt gtcgattaac gggaaaaagg caggtgtcgg
     1021 taccagcgct tcggtcacaa tggtgagatg cgtttcccaa ccgcgcgcga gggcttccgc
     1081 atcggtagtc agtttatctg cggcttccag caaaacgcgc cctcgctcca gcaacatcct
     1141 cccgacattg gtgaatttgg tgcgatggcc cgagcggtca aacagcacca catccagctc
     1201 ttcttccagt ttttgcatgg tgtagctaag tgcagaaggc acgcgtccca gctcatccgc
     1261 cgccgccgca aaactgcccc ggcgatcgat cgcatccata acccgtagtg cttccagcgt
     1321 taatgccctt tctttggcca tttcgttctc attcaggaaa tttgaacata ccgggcagaa
     1381 tatctggcta acaatgtagc gtccagcccc ttaccataaa aggaagtaaa gagaggtcaa
     1441 gaattatgat tactacccga actgccaggc agtgtggaca agcagactac ggatggttgc
     1501 aggcccggta tactttttcc tttggacact acttcgaccc gaaattgtta ggctatgcct
     1561 ccctgcgtgt gcttaaccag gaagtgctgg ccccagatgc cgcctttcag ccgcgaacct
     1621 atcccaaagt cgatatttta aatgtgattc tggatggaga agcagagtat cgcgacagcg
     1681 aaggcaatca tgttcaggcc agcgccggtg aggcgttgct gctctctacc cagccgggtg
     1741 tcagctacag cgaacacaat ctcagcaaag acaaaccgtt aacacgaatg cagctttggc
     1801 tggacgcctg cccgcagcga gagaatccgc tgattcaaaa actggcgctt aatatgggca
     1861 agcagcaatt aatcgcctcg ccagagggta cgatgggaag cctgcaatta cgccagcaag
     1921 tgtggctgca ccatatcgtg ctcgacaaag gcgaaagtgc gaatttccag ttgcatgggc
     1981 cacgcgcgta tttgcaatcg attcacggga aatttcatgc gcttacgcat catgaagaga
     2041 aagcggcgct gacctgcggt gatggggcgt ttattcgtga cgaggctaac attacgctgg
     2101 ttgccgattc cccactgcgc gctttgctga tagatttgcc tgtctagttg tttttacagg
     2161 gagatgatga tgagtaaaaa atcggccaaa aagcgccagc cagtgaagcc cgtggtggcg
     2221 aaagaacctg ctcgcaccgc caaaaatttt ggctatgaag agatgttgag cgagctggaa
     2281 gccatcgtcg cggatgctga aacgcgttta gccgaggatg aagctaccgc gtaaagacag
     2341 gttgccggat gcggcgaaaa cgccttatcc ggcctacaaa atccaacaac attaacgaat
     2401 taaacaaccc gtaggccgga taagatgcgc cagcatcgca tccggcatca ggtacaaaac
     2461 acaacaacat caacaagttg agcaccccgt aggccggata aggtgcgcca gcatcgcatc
     2521 cggcacgata cccaaaacct ggcgtttatc tggccttgct cgccataatc tcgataatct
     2581 gccgatccgt ttgctgcatc gaatggcttg ctaacgcaca caggttggca atcgactgct
     2641 caacatcatg cgccacgatc ccttcattgc cggtcacggc ggtatcatcc agcgccatta
     2701 acaccgcttt ccacgcagcc gaagcactgg tcgaaacctt catcgcgcag ctgttcgacg
     2761 caccatcgca aatcatgccg ctgacatcgc cgatcatact gctgatcgcc atcgagatgg
     2821 tttcataacg cccatccacc agccatgcca tcccggcggc ggcccccatt gctgcggtcg
     2881 ttgcggcaca cagcgcagac aaacgcggta actggttatg gatgtaaatt gcgctcaaat
     2941 gagaaagcat cagcgcacgc gccagccgtt catcatccgc tccgaagtgt tctgctacca
     3001 ccaccacagg cattgttgcg gtgatcccct ggttacccga gccggagtta ctcatagccg
     3061 gaagcgtagc gccgcccata cgcgcatcgg atgccgcgct ggtacgaatc acaatggatg
     3121 aagagagatc tttcgccagc aaaccgcgct cgcactgttt ttccagcgtc gcgccaatat
     3181 gcagccccca cttaccgctc aaaccttcct gcgataacgc acaatttagc ttcgcggaat
     3241 cgagaataaa gcggatcgcc gcaaacggga cttcattgac gaacttcagg atctcagcca
     3301 gcgtcgttct ggaaagcacc gtcagcggag actcttgctc gccctctgcc acacacgcct
     3361 gctgggtaaa caccacacca ttgtgcgtct caatatgcac aatgttggta tgcccgccga
     3421 cgatggtgac acacgcccac ttctcaccgt tccagacttt ggcgcgtgag aagaggattt
     3481 cattgcaagg ttcctggatc ttaacggaga ctttccccgc cgccagcagt gctttggcat
     3541 cggcaattgc ctgcgcagtt gcgtctttca gcacttccag cccggcgttg gcatttccac
     3601 ctaacgcccc cagcgccgcc gcaatcggca gccccaccat tcccgtgccg ggaacggtga
     3661 cgcccagacc gttcttcatc agatttggcg aaacccaggc ttctacacgt tcaaccggac
     3721 cttccagttc tgccgcagca accgccgccg ccagcgccag tgaaatcggt tcagtacatc
     3781 ccagcgccgg ttttacttcc tcctgaacgg cgaggatgta acgctgccat aacggattta
     3841 aagtcgaatc aaacatatta aaaaccttaa aatttcaggt aaatcaagaa aatgccagga
     3901 acggagaaac acaaagcaac aaaccagtga cgataatcag gtacagagac atccctttgt
     3961 atttgtgcaa tgccggtact ttgtaaacca gccacgccgg gatcaggcac cctaccatgc
     4021 cgaaaatcgg gctacagata gaggtgaagc tcaacaccgg tgcgttcagt acgatggcgc
     4081 tccaggccag caaaatggcg aaaatcatga tgccgcgctg aacaagattt tcgttaatct
     4141 tctcggcagg catcttgcga cgcaggatgt tcattacgat cccttgcgtt gcttcgcgaa
     4201 agcctaaata gacgccaaag aatgcagtca tgacagcaaa gatattgaga atgacgctga
     4261 ccactttcac ccacgctgcg ccgtcaccgc taataaactg tgcggcaatc gccagcgcgg
     4321 aaatattctg ctcataggct tttaccgctt cgtcatgtcc catcgccagc gtgaacgaca
     4381 cggcgtagaa aaagacggtg acaaacaaaa tgccaaacgc gatattcatt gcccgcaatg
     4441 ctttatgccg cgccacttca attgattttt cccgcgagcg ataagagatc accatcgggc
     4501 ttaacgtctg gataaacaga atcgacgtca gggtaaacgg cagcgtaata atggcgtttt
     4561 tcaccagcag ccccagcggc ggtagcgaac cgacgttata cagatgccac attcccacca
     4621 tcgacacgcc cagcgccgcg accaccagca gcttggtcag caccatgccg gtcgaaattt
     4681 tgaataacaa tttctcgccg cgtgaagaga tcgccaccag aatgcaaatc agtaccagac
     4741 cataaaaggg actgtctgac agcaaccctt ccgtcacgcc gaaggtatgc agataggaag
     4801 cactatcgtt ggtgatggcg gtggaataga cgaacatcca aatcaccagc attacgaaat
     4861 agagcgcacc taacaggatg ccccagtttt tacctaaata accgctaatg acactcgggt
     4921 aatctttaca ttctggtgat tctgccagcg tattaataaa caaccgctga aacagataca
     4981 ttgccgggta accaatcacc gatgagagca aaaataccca caatcccatc aaaccgacct
     5041 gcaccgggag aaaaacaatc cccgcgccaa tcgccatccc gatactcata atcacccagc
     5101 cggtgtcagt actgtcgaat ttgatcgctt ctcgccactc gctctcactc attccagcac
     5161 gacccgccgg ggtcgaagcg tctgcaatga cgcctttatt cgatgcaatt tccataattt
     5221 ctcgctcaat attttgtagg gcttattatt tttttccgag ccgcatcaag gcgatatgcg
     5281 gtctacgttt ttgcaggcga gttattagaa taaagaaatg atacgcgcga ggtcagagaa
     5341 aatcttcaca atcaagactt tgaaataacg gagatggata agaattttct actaaattaa
     5401 tcgcaagaga atattcattc tctatttgcg gcagatcaca aaaaaaaggt gcacatttgt
     5461 gcacccaagg atgaaagctg acagcaatgt cagccgcaga ccactttaat ggccagtcct
     5521 ccgcgtgatg tttcgcggta tttatcgttc atatctttgc cggtttcata catcgtctcg
     5581 atcactttat cgagtgaaac acgcggtgcc gaggtgcggc gcatcgccat ccgcgcggcg
     5641 tttactgctt tcacggcatt aatggcatta cgttcaatgc acgggatttg tacctgtccg
     5701 gcaaccggat cgcaggtcag cccaaggtta tgctccatcg cgatttccgc cgcattgcat
     5761 acctgcgccg gactgccgcc cagtagttca gttaaccctg ccgccgccat tgaacaggcc
     5821 acgccaatct ccccctgaca gccgacctcc gcgccagaga tggaggcgtt cattttatac
     5881 agcgcgccaa tagcccccgc tgccagaaaa tagcgggcaa ttgaccgctc gtttaccgga
     5941 cgacggaact tatcgtaata agccagcact gccggaataa tgccgcacgc accgttagtc
     6001 ggtgccgtta ccacgcgccc gccagctgcg ttttcttcac taaccgccag cgcgtacatg
     6061 ttgatccagt cgatgacatt catcggatcg ttagagatgt tatcgctgga aaccagctga
     6121 cgacgcagcg ctacggcacg acgcggcaca ttgagcggac caggcagcac gccttcagtg
     6181 ttcatcccgc gttcaatacc gtcatgcatc acttgccaga tacgggcaaa ccctgcgtca
     6241 atttccgctt tgctgcgcag cgccagctcg ttgtgcatca tcaggccaga tatagacagg
     6301 ccgttgtaat cacacatttt cagcagttca cctgctgagt ggaaatcgta aggtacggac
     6361 gtttcgacat cgtgcgacag gccgaagtgt tcttcttcga caataaaccc gccgccgaca
     6421 gagtaatagg ttttacttaa tagcgcttcc tgtcccttcc aggcagtgat ccgcattccg
     6481 ttctcatggc gaggcaacat ttcgggatgg aagataatgt tctttgctac aggaaaatca
     6541 acaatatgcg caccagatgc cactggcagc cgcccgctgc gcgttactaa ctctataaat
     6601 gcagggatct catcaatgac aacatcctgc ggactgtttc ctgccagtcc catgataatg
     6661 gcgacatccg tggcatggcc tttgcccgtc agtgacaacg acccatacag atcgaccaca
     6721 atatggctcg tcgcggttaa taagccgcta ctttccagcc gatcaataaa actttttccg
     6781 gcattcattg gccccacggt atgcgaactg gagggaccaa tcccaatttt gaaaatatcg
     6841 aatgcactaa tcatatccac accctcggat tgccgttcag tgaagtggag cggaacgacc
     6901 ttgcgaccgt cccgctcacg aggctttacg cactacgtac tgcgatagct tcaatttcca
     6961 gcttcacatc tttcggcaaa cgcgcaacct gcacacagct ccgggtcggg taggtcgcct
     7021 gatgctcatc gaagaactgc ttatagactt cattgatggt ggcaaaatca ttcagatcgg
     7081 tgatgaacac ggtcatcttg atgatatcgc ccacgctcag cccggcagca accacgatcg
     7141 ctttgacgtt ttcgaggctt aaacgcgcct gatcttgcac atcagccggg atctcaccgg
     7201 tctgcgggca aaccggaatt tgcccggagg tgaagaccat gctgcctaaa tcaacgccct
     7261 gaacataagg gccgattgcg cctggggcac gttgcgtttc gataatcttt ttcatacatc
     7321 ctccggcgtc agagcgcctg ggtaaaagta cgtgaaataa catcctgttg ctgttcacgg
     7381 gtcagtgcgt tgaagcgcac ggcgtagcca gagacacgga ttgtcaggtt aggatatttt
     7441 tccgggtgct cgatggcatc cagcagcatt tcccgattca ttacgttgac gttgaggtgt
     7501 tgaccgcctt cgacatccgc ttcgtggtgg aaatacccat ccagcaggcc gacaaggttg
     7561 gttttacgta ctggatcttc tttgcccagc gccgcaggaa cgattgagaa ggtgtacgag
     7621 atcccatctt tggcgtaggt gaacggcagt ttcgccaccg acgtcaatga ggccacggca
     7681 cctttgcggt cacgaccatg catcgggtta gcgcccggcg cgaacggtgt tccggcgcga
     7741 cgaccgtccg gcgtattacc ggttttctgg ccgtacacca cgttagaagt gatagtcaga
     7801 atcgactggg tagtgacggc gttgcgatag gttggcagcg ctttaatttt cttcataaag
     7861 cgttcaacca ggtcgcaggc aatgctgtct acgcgctcgt cgttgttgcc gtactgcgga
     7921 tattcaccgt cgatttcaaa gtccaccgcc aggccgtttt cgtcacggat tggtttcacg
     7981 cgggcatatt tgatggcaga cagggagtcc gtcgccaccg acaggcccgc gatgccgcat
     8041 gccatagtgc gatagacatc acgatcgtgc agcgccatca gcgaagcttc gtagctgtac
     8101 ttgtcgtgca tgtagtgaat gatattcagc gcgctgatgt actgcaccgc cagccagtcc
     8161 atgaagtgat cgaggctgtc catcactttg tcgtaatcca gcacgtcgtc catcagcggt
     8221 gctgttttcg gcccgacctg aatcttcagc ttctcgtcca ccccgccgtt aattgcgtag
     8281 agcagcgttt tcgccaggtt agcgcgtgca ccaaagaact gcatttgctt accaatcacc
     8341 attgggctga cgcagcaggc aatcgcgtaa tcgtcgctgt tgaagtcagt acgcatcaga
     8401 tcgtcatttt catactgcaa ggaagaggtg acgatcgaca cctgcgcggc atattttttg
     8461 aaggcaatcg gtaattcttc cgaccaaaga atggtcaggt taggttccgg tgccggcccc
     8521 atagtgtgca gggtgtgcaa atagcggaag gagtttttgg tcaccagcgt acgaccgtcc
     8581 agccccatcc cgccgatcac ttccgtcgcc cagattgggt cgccggagaa cagcgaatca
     8641 aattccggtg tacgcaggaa gcgtaccata cggatcttca tgatgaagtg atcgatcagt
     8701 tcctgtgcct gctgctcatt gagtacgcca gctttaaagt cgcgctcaat gtagatatcg
     8761 aggaacgatg ccgtgcggcc cagcgacatc gcgccgccat tttgcgattt cactgccgcc
     8821 agataagcga agtagagcca ctgcaccgct tcctgcgcat tctgcgccgg gcgagagata
     8881 tcaaagccat atttcgccgc catttcctga atctgcaaca gcgcatgacg atgctctgcc
     8941 agctcctcac gcagacggat ggtggcttcc agatcctcgc ctttttccag acgagactgg
     9001 agatcggcaa attgcagttc gcgttcacgt accagataac tgatgccata cagcgctacg
     9061 cggcgatagt caccgataat gcgcccacgg ccatagccat ctggtaaacc ggtcagcacg
     9121 ccagatttac ggcagcgcag catatccggt gagtaaacat caaatacgcc ctggttatgg
     9181 gttttacgca gatcggtaaa cagatattca aattcactgt ccatttcacg gccataggcg
     9241 tggaatgaac ttttaatcat attaatgcca ccgaacgggt gtagcgcacg tttcaacggc
     9301 gcatccgttt gcaggccaac aattttttcc agcggctggt taatatatcc cgcatcatga
     9361 gcggtaattg tggtggcaat attggtatcg aaatcaaccg gcgcgtgggt tgcgttttcg
     9421 atacggatac cttccattac tttttcccac aattccgtgg tggcaggcgt cgcttcggcg
     9481 aggaaagatt catcgccttc atacggtgta tagttatgtt gaataaaatc gcggacatta
     9541 atttcgtttt tccagtccgt acctttaaag ccaagccatg cgtcggcgta cagcttatcg
     9601 ctggtatcaa tatctacctt catgaaaaat aatctctcta caatacttca actaaattat
     9661 gcaaattctg cgggcgcgtt aactttgcct aaatgaatgg catccaaagc aatcattttt
     9721 tcttcgttag tcggaataac agcgcaaatg acacgcgcat tttcactgga aacaattcgc
     9781 tcaccacagg agttagagcg attattcatt tctgtatcaa tctctacgcc taatacagcc
     9841 aaatgttcca tgaccagacg acgaattaag cttgaattct ctcctattcc gccggtgaat
     9901 ataattccat ccaggcgacg taatgaagct gcgtgtccgg caatatgacg ggcaattcgg
     9961 tgaacaaagg ttttaattgc cagttgcgcg cgttcgtgac cttcatgcca ggctttttcc
    10021 agaacacgta aatccgaaga aagaccggaa atacctaata atcccgactc tttattcact
    10081 acgcgttcca ggtcacccag gctctggttg gtttggctgg cgacccagga catcgcacca
    10141 aagtcgacat cgccactgcg ggtacccatc atcaagcctt ccagcggcgt cattcccatt
    10201 gaagtatcaa cactctgacc gttgcgaacc gcgcagattg acgcgccatt gccaagatgc
    10261 gccacaacca ggccggagtc atcttccgcc agattcagca gcgaatgtgc gcgctgggaa
    10321 acatagcggt gcgacgtgcc gtggaaacca taacggcgta cacctaactc ttcataatat
    10381 ttccacggca ggccgtataa ataagcttcc ggagccatcg tctggtggaa actggtatca
    10441 aataccgcca cctgagttac gcccggaaat aattgctgcg ccgattcaat accacttaaa
    10501 ttggcgtaat tatgcagggg tgccagtgga gaaacgcgac ggatattatc aatgacttca
    10561 tcggtaataa tggcggactc ggtaaaaata ctgccgccgt gagcgatgcg gtggccaatt
    10621 aaggccacac tgtcatttaa attccgtttt tccagttcaa atgcaattgc cttcaatgca
    10681 ccttcgtagc tgtggtgagc cagcggtgct ggctctcccc catttacgga taagaatgca
    10741 ttttccgagt taataccgtc ggcaatacct gacattaata cttcacagtc gctggcatcg
    10801 agtacggaaa acttaatcga agacgaacca cagttaataa ccaaaacaac cggaaattca
    10861 ttcatctctt ttctcatcct gagttacgga ttaaaacagt ttgtatacga tgttcaggat
    10921 ggtcagcaga ccaatcacgg taacaaacac gttatccaga cgaccacggt atttcgccag
    10981 agacggcgct ttacggatgg catacatcgg caacaggcac agcagggatg cgataatcgg
    11041 tgcgcccatg gcttcaataa ggtcgaggat gttcgggttg gcgtaggcaa caacccaggt
    11101 ggagcccatg atgaagatca tgctgatagt gttcagttta cccagcgaca ctttagtttt
    11161 gtcgccttta taaccgaact tcagaatcag accattcaag ccttccagcg tccccagata
    11221 gtgaccgaag aaagatttga agatagccac gagtgcgatg atggaagccg catattccag
    11281 tgtaatcgcg aacgttgttt tggtaccggt catggacgca aagtggttag ccagataaga
    11341 aagcactgga atattctgcg ctttggcttc cgccatgttg gccggagaca gcgtaaacag
    11401 gcagctaaag gcaaagaaca tcaccactgc aaccatcagc atgctggcac gagaaatgat
    11461 ttgggaacat ttacgttcgg tgaagtcgcg accgaagtct ttctcatact cttcacgttt
    11521 agaaaccacg aaggaagaga cgattggcga gaagttaaag gagaaaacca tgatggaaat
    11581 ccccagccag acagtgatca ggataccgtc atgaccggtt aacgacagcg aaccgaggtc
    11641 aacctggtcg ataactgcag agttccagta agggatcagc gacagagaaa tcagcaccag
    11701 gctggcgata aacggccata ccaggtagct catcacttta accatcagat ccttaccaaa
    11761 ccagatgacg aaagccatca gcagcaacag gaacagcgcc acaaagccgc gattcagcgg
    11821 tgcaaatccg agctggtttt cccagaacgt cataaaggta ttggtaatag taacgccata
    11881 aatccacagc agtgggcaaa tcgcgaagaa gtacaggaac gtgataacca cgccgccagt
    11941 tttaccaaaa tgctcttcca ccgtttccgt aatgttgccg gaagggttag agccagaaag
    12001 acacagacgc gccagcgccc ggtggcaata aaatgcgatg gggtatgcca ataccaacat
    12061 cagaagaatc gggatcagtc cgccaaaacc tgcgcggata gggaagaaca gcaccccggc
    12121 gccgattgcc gtaccaaaca agcctaacgt ccatgtggta tctgatttac gccaggacga
    12181 ttgttttgtc tggctggata caatgctatc tgaagtactc atatcctatc ctcaacgaat
    12241 taattaagcg tcaacgaaac cggtgatttg agagacgcga gaaagatcga tattgccgcc
    12301 ggaaataata ctgacggttt ttctgttttg aatatattgg tctaatttac cgcttaataa
    12361 tgcagcacat gccagagcgc ctgcgccttc ggtgacgact ttattgcgct gaattaaggc
    12421 aatcatactg tttctgattt cgtcttcgct gaccagcacg atgtcatcga ctaattcacg
    12481 aacgatttcg taagttaaat tacccgggcg ggagacatca caaccatccg ccagggtgcc
    12541 ggtagttcgg tgcgtggtta tttctccgga gtggaaagaa gccgccatgc cgtgaacgtt
    12601 ttcagactgt acgccaataa cacgaatggt cgggttaata gatttaattg ccaccgcaat
    12661 accagcaatt aaaccgccac caccaattgg cacaatcacg ttatcgacat catagagatc
    12721 ttccataatt tccagaccaa tcgttccctg gccagcaatc actttcggat catcgtaagg
    12781 tgggataaaa atacggcctt ccatttcgac aatttcgctc actttagcga tagtgtcgtt
    12841 gaaattatca ccatgcagaa cgacttctgc ggagtagtcg cacgttgccg ctactttgga
    12901 ttttggcgca ccttttggca tcaccacttt accgtcgata cccagcatcg cgcaggagag
    12961 cgaaacccct tgcgcatggt tgcccgcaga acaggccact acacctttac gtttttccgc
    13021 atcagtcagt gaacttaatt tattaaatgc gccacgaatt ttaaatgaac ccgtacgctg
    13081 catattttca aacttcagga atatttcacc tttgcaacgt tcactaaaat agtttgagcg
    13141 aggcatgcct gttttataaa ttcgcccagc cagtcgttgt ttcgcttcaa taatgtcatc
    13201 aatagcaacc ggcagatcgt atgtaatatg cattataaaa cctcttcgcc tgaattaaat
    13261 atgtaggtaa ccgtaaccga cacctgcaaa acagacaggt ggattattta tgtttaaata
    13321 aaaacaaata actaaccaac ttctat
//
LOCUS       DAZKHF020000118        13309 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2201S31241
            SAMN43267800-rid25106413.denovo.119, whole genome shotgun sequence.
ACCESSION   DAZKHF020000118 DAZKHF020000000
VERSION     DAZKHF020000118.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267800
            Sequence Read Archive: SRR30307263
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 13309)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13309)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 13309)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350581.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350581.2
            Genome Coverage       :: 151x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:21:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,652
            CDSs (total)                      :: 5,557
            Genes (coding)                    :: 5,320
            CDSs (with protein)               :: 5,320
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 237
            CDSs (without protein)            :: 237
            Pseudo Genes (ambiguous residues) :: 0 of 237
            Pseudo Genes (frameshifted)       :: 102 of 237
            Pseudo Genes (incomplete)         :: 130 of 237
            Pseudo Genes (internal stop)      :: 52 of 237
            Pseudo Genes (multiple problems)  :: 41 of 237
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13309
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267800-rid25106413.denovo.119"
                     /serotype="O 26:H11"
                     /isolate="2201S31241"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-01"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            complement(<1..1244)
                     /locus_tag="ACHOSC_RS13880"
                     /old_locus_tag="pgaptmp_002776"
     CDS             complement(<1..1244)
                     /locus_tag="ACHOSC_RS13880"
                     /old_locus_tag="pgaptmp_002776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015572536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HGU4548686.1"
                     /translation="MKLNARQVETAKPKEKTYKMADGGGLYLEVSAKGSKYWRMKYRR
                     PSDKKEDRLAFGVWPMVTLAQARAKRDEAKKLLVQGIDPKAEQKEAQAENSGAYTFET
                     IAREWHASNKRWSEDHRSRILRYLELYIFPHIGSSDIRQLKTSHLLAPIKKVDASGKH
                     DVAQRLQQRVTAIMRYAVQNDYIDSNPASDMAGALSTTKARHYPALPSSRFSEFLARL
                     AAYRGRVMTRIAVELSLLTFVRSSELRFARWDEFDFDKSLWRIPAKREEIKGVRYSYR
                     GMKMKEEHIVPLSRQAMVLLEQLKQISGDKELLFPGDHDAAKVMSENTVNSALRAMGY
                     DTKTEVCGHGFRTMARGALGESGLWSDDAIERQLSHTERNNVRAAYIHTSEHLDERRL
                     MVQWWADFLEANRNNSVTPYEFA"
     gene            complement(1408..1492)
                     /locus_tag="ACHOSC_RS13885"
                     /old_locus_tag="pgaptmp_002777"
     tRNA            complement(1408..1492)
                     /locus_tag="ACHOSC_RS13885"
                     /old_locus_tag="pgaptmp_002777"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1456..1458),aa:Leu,seq:caa)
     gene            1688..2707
                     /gene="ahr"
                     /locus_tag="ACHOSC_RS13890"
                     /old_locus_tag="pgaptmp_002778"
     CDS             1688..2707
                     /gene="ahr"
                     /locus_tag="ACHOSC_RS13890"
                     /old_locus_tag="pgaptmp_002778"
                     /EC_number="1.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418690.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent aldehyde reductase Ahr"
                     /protein_id="HGU4548687.1"
                     /translation="MSMIKSYAAKEAGGELEVYEYDPGELKPQDVEVQVDYCGICHSD
                     LSMIDNEWGFSQYPLVAGHEVIGRVVALGSAAQDKGLQVGQRVGIGWTARSCGHCDTC
                     ISGNQINCEQGAVPTIMNRGGFAEKLRADWQWVIPLPENIDIESAGPLLCGGITVFKP
                     LLMHHITATSRVGVIGIGGLGHIAIKLLHAMGCEVTAFSSNPAKEQEVLAMGTDKVVN
                     SRDPQALKALSGQFDLIINTVNVSLDWQPYFEALTYGGNFHTVGAVLTPLSVPAFTLI
                     AGDRSISGSATGTPYELRKLMRFAARSKVAPTTELFPMSKINDAIQHVRDGKARYRVV
                     LKADF"
     gene            2837..4339
                     /gene="yjgR"
                     /locus_tag="ACHOSC_RS13895"
                     /old_locus_tag="pgaptmp_002779"
     CDS             2837..4339
                     /gene="yjgR"
                     /locus_tag="ACHOSC_RS13895"
                     /old_locus_tag="pgaptmp_002779"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helicase HerA-like C-terminal domain-containing
                     protein"
                     /protein_id="HGU4548688.1"
                     /translation="MSEPLLIARTPDTELFLLPGMANRHGLITGATGTGKTVTLQKLA
                     ESLSEIGVPVFMADVKGDLTGIAQAGTASEKLLARLKNIGVNDWQPHANPVVVWDIFG
                     EKGHPVRATVSDLGPLLLARLLNLNDVQSGVLNIIFRIADDQGLLLLDFKDLRAITQY
                     IGDNAKSFQNQYGNISSASVGAIQRGLLSLEQQGAAHFFGEPMLDIKDLMRTDANGKG
                     VINILSAEKLYQMPKLYAASLLWMISELYEQLPEAGDLEKPKLVFFFDEAHLLFNDAP
                     QVLLDKIEQVIRLIRSKGVGVWFVSQNPSDIPDNVLGQLGNRVQHALRAFTPKDQKAV
                     KAAAQTMRANPAFDTEKAIQELGTGEALISFLDAKGSPSVVERAMVIAPCSRMGPVTE
                     DERNGLINHSPVYGKYEDDVDRESAYEMLQKGVQASIEQQNNPPAKGKEIAVDDGILG
                     GLKDILFGTTGPRGGKKDGVVQTMAKSAARQVTNQIVRGMLGSLLGGRRR"
     gene            complement(4458..5540)
                     /gene="lptG"
                     /locus_tag="ACHOSC_RS13900"
                     /old_locus_tag="pgaptmp_002780"
     CDS             complement(4458..5540)
                     /gene="lptG"
                     /locus_tag="ACHOSC_RS13900"
                     /old_locus_tag="pgaptmp_002780"
                     /EC_number="7.5.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313266.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0015221 - lipopolysaccharide transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter permease LptG"
                     /protein_id="HGU4548689.1"
                     /translation="MQPFGVLDRYIGKTIFTTIMMTLFMLVSLSGIIKFVDQLKKAGQ
                     GSYDALGAGMYTLLSVPKDVQIFFPMAALLGALLGLGMLAQRSELVVMQASGFTRMQV
                     ALSVMKTAIPLVLLTMAIGEWVAPQGEQMARNYRAQAMYGGSLLSTQQGLWAKDGNNF
                     VYIERVKGDEELGGISIYAFNENRRLQSVRYAATAKFDPEHKVWRLSQVDESDLTNPK
                     QITGSQTVSGTWKTNLTPDKLGVVALDPDALSISGLHNYVKYLKSSGQDAGRYQLNMW
                     SKIFQPLSVAVMMLMALSFIFGPLRSVPMGVRVVTGISFGFVFYVLDQIFGPLTLVYG
                     IPPIIGALLPSASFFLISLWLLMRKS"
     gene            complement(5540..6640)
                     /gene="lptF"
                     /locus_tag="ACHOSC_RS13905"
                     /old_locus_tag="pgaptmp_002781"
     CDS             complement(5540..6640)
                     /gene="lptF"
                     /locus_tag="ACHOSC_RS13905"
                     /old_locus_tag="pgaptmp_002781"
                     /EC_number="7.5.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0015221 - lipopolysaccharide transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter permease LptF"
                     /protein_id="HGU4548690.1"
                     /translation="MIIIRYLVRETLKSQLAILFILLLIFFCQKLVRILGAAVDGDIP
                     ANLVLSLLGLGVPEMAQLILPLSLFLGLLMTLGKLYTESEITVMHACGLSKAVLVKAA
                     MILAVFTAIVAAVNVMWAGPWSSRHQDEVLAEAKANPGMAALAQGQFQQATNGSSVLF
                     IESVDGSDFKDVFLAQIRPKGNARPSVVVADSGHLTQLRDGSQVVTLNQGTRFEGTAL
                     LRDFRITDFQDYQAIIGHQAVALDPNDTDQMDMCTLWNTDTDRARAELNWRITLVFTV
                     FMMALMVVPLSVVNPRQGRVLSMLPAMLLYLLFFLIQTSLKSNGGKGKLDPTLWMWTV
                     NLIYLALAIVLNLWDTVPVRRLRASFSRKGAV"
     gene            complement(6703..6753)
                     /gene="ytgA"
                     /locus_tag="ACHOSC_RS13910"
                     /old_locus_tag="pgaptmp_002782"
     CDS             complement(6703..6753)
                     /gene="ytgA"
                     /locus_tag="ACHOSC_RS13910"
                     /old_locus_tag="pgaptmp_002782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YtgA"
                     /protein_id="HGU4548691.1"
                     /translation="MSIIILATISTTQENK"
     gene            6907..8418
                     /gene="pepA"
                     /locus_tag="ACHOSC_RS13915"
                     /old_locus_tag="pgaptmp_002783"
     CDS             6907..8418
                     /gene="pepA"
                     /locus_tag="ACHOSC_RS13915"
                     /old_locus_tag="pgaptmp_002783"
                     /EC_number="3.4.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030145 - manganese ion binding [Evidence
                     IEA];
                     GO_function: GO:0070006 - metalloaminopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0019538 - protein metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="leucyl aminopeptidase"
                     /protein_id="HGU4548692.1"
                     /translation="MEFSVKSGSPEKQRSACIVVGVFEPRRLSPIAEQLDKISDGYIS
                     ALLRRGELEGKPGQTLLLHHVPNVLSERILLIGCGKERELDERQYKQVIQKTINTLND
                     TGSMEAVCFLTELHVKGRNNYWKVRQAVETAKETLYSFDQLKTNKSEPRRPLRKMVFN
                     VPTRRELTSGERAIQHGLAIAAGIKAAKDLGNMPPNICNAAYLASQARQLADSYSKNV
                     ITRVIGEQQMKELGMHSYLAVGQGSQNESLMSVIEYKGNASEDARPIVLVGKGLTFDS
                     GGISIKPSEGMDEMKYDMCGAAAVYGVMRMVAELQLPINVIGVLAGCENMPGGRAYRP
                     GDVLTTMSGQTVEVLNTDAEGRLVLCDVLTYVERFEPEAVIDVATLTGACVIALGHHI
                     TGLMANHNPLAHELIAASEQSGDRAWRLPLGDEYQEQLESNFADMANIGGRPGGAITA
                     GCFLSRFTRKYNWAHLDIAGTAWRSGKAKGATGRPVALLAQFLLNRAGFNGEE"
     gene            8772..9215
                     /gene="holC"
                     /locus_tag="ACHOSC_RS13920"
                     /old_locus_tag="pgaptmp_002784"
     CDS             8772..9215
                     /gene="holC"
                     /locus_tag="ACHOSC_RS13920"
                     /old_locus_tag="pgaptmp_002784"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004098129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit chi"
                     /protein_id="HGU4548693.1"
                     /translation="MKNATFYLLDNDTTVDGLSAVEQLVCEIAAERWRSGKRVLIACE
                     DEKQAYRLDEALWARPAESFVPHNLAGEGPRGGAPVEIAWPQKRSSSPRDILISLRTC
                     FADFATAFTEVVDFVPYEDSLKQLARERYKAYRVAGFNLNTATWK"
     gene            9215..12070
                     /gene="valS"
                     /locus_tag="ACHOSC_RS13925"
                     /old_locus_tag="pgaptmp_002785"
     CDS             9215..12070
                     /gene="valS"
                     /locus_tag="ACHOSC_RS13925"
                     /old_locus_tag="pgaptmp_002785"
                     /EC_number="6.1.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000416368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004832 - valine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006438 - valyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="valine--tRNA ligase"
                     /protein_id="HGU4548694.1"
                     /translation="MEKTYNPQDIEQPLYEHWEKQGYFKPNGDESQESFCIMIPPPNV
                     TGSLHMGHAFQQTIMDTMIRYQRMQGKNTLWQVGTDHAGIATQMVVERKIAAEEGKTR
                     HDYGREAFIDKIWEWKAESGGTITRQMRRLGNSVDWERERFTMDEGLSNAVKEVFVRL
                     YKEDLIYRGKRLVNWDPKLRTAISDLEVENRESKGSMWHIRYPLADGAKTADGKDYLV
                     VATTRPETLLGDTGVAVNPEDPRYKDLIGKYVILPLVNRRIPIVGDEHADMEKGTGCV
                     KITPAHDFNDYEVGKRHALPMINILTFDGDIRESAQVFDTKGNESDVYSSEIPAEFQK
                     LERFAARKAVVAAVDALGLLEEIKPHDLTVPYGDRGGVVIEPMLTDQWYVRADVLAKP
                     AVEAVENGDIQFVPKQYENMYFSWMRDIQDWCISRQLWWGHRIPAWYDEAGNVYVGRN
                     EEEVRKENNLGADVALRQDEDVLDTWFSSALWTFSTLGWPENTDALRQFHPTSVMVSG
                     FDIIFFWIARMIMMTMHFIKDENGKPQVPFHTVYMTGLIRDDEGQKMSKSKGNVIDPL
                     DMVDGISLPELLEKRTGNMMQPQLADKIRKRTEKQFPNGIEPHGTDALRFTLAALAST
                     GRDINWDMKRLEGYRNFCNKLWNASRFVLMNTEGQDCGFNGGEMTLSLADRWILAEFN
                     QTIKAYREALDSFRFDIAAGILYEFTWNQFCDWYLELTKPVMNGGTEAELRGTRHTLV
                     TVLEGLLRLAHPIIPFITETIWQRVKVLCGITADTIMLQPFPQYDASQVDEAALADTE
                     WLKQAIVAVRNIRAEMNIAPGKPLELLLRGCSADAERRVNENRGFLQTLARLESITVL
                     PADDKGPVSVTKIIDGAELLIPMAGLINKEDELARLAKEVAKIEGEISRIENKLANEG
                     FVARAPEAVIAKEREKLEGYAEAKAKLIEQQAVIAAL"
     gene            complement(12124..13308)
                     /gene="yjgN"
                     /locus_tag="ACHOSC_RS13930"
                     /old_locus_tag="pgaptmp_002786"
                     /pseudo
     CDS             complement(12124..13308)
                     /gene="yjgN"
                     /locus_tag="ACHOSC_RS13930"
                     /old_locus_tag="pgaptmp_002786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313261.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="YjgN family protein"
BASE COUNT         3389 a         3428 c         3500 g         2992 t
ORIGIN      
        1 gcaaattcat aaggtgtaac agagttattt cgattggcct caagaaaatc agcccaccat
       61 tgaaccatta atctacgttc gtctaaatgc tcagaggtat gaatatatgc tgcacgcaca
      121 ttgttacgct ctgtatgact caactgccgt tctatcgcgt catcgctcca taagcctgat
      181 tcaccaagcg cgccacgcgc catcgtccta aatccatgcc cacagacctc agttttcgtg
      241 tcatacccca tcgcacgcaa ggcgctattt actgtgttct cactcataac tttggccgcg
      301 tcgtgatccc ctggaaaaag taactcttta tcgccactaa tctgcttgag ttgctctaac
      361 aaaaccatcg cctgtcgact aagaggaacg atatgctcct ctttcatctt catgccacga
      421 tacgagtaac gcacgccttt aatttcttct cgctttgcag gtatacgcca aagagattta
      481 tcgaagtcga actcatccca tcgcgcgaaa cgtaactcac tggaacgtac aaaagttagt
      541 aaggaaagct cgaccgcgat ccttgtcatt acacgaccac gatatgcagc aagacgtgca
      601 agaaactcag agaagcggct agagggtaaa gctggatagt gtcgtgcttt ggttgtagat
      661 aacgcaccgg ccatgtcact ggctggatta gagtcgatgt aatcgttctg tacggcataa
      721 cgcataatgg ccgtgacgcg ctgttgcagg cgctgagcga catcgtgttt gccactggca
      781 tcaacttttt taatcggggc taacaggtgg ctggttttaa gctgccgaat gtcggacgaa
      841 ccgatatgag gaaaaatata aagctcaaga tagcgcagaa tgcgtgatcg atggtcttca
      901 ctccagcgct tattactggc atgccattca cgagcaattg tttcgaaagt gtatgcgccc
      961 gaattctcag cctgagcttc tttctgctcg gcttttgggt caatgccctg cactaagagc
     1021 tttttagcct cgtcacgctt tgctcttgcc tgagcaagtg tcaccatggg ccaaacacca
     1081 aaagcgaggc gatcctcttt tttgtcagag gggcgtctgt atttcatgcg ccagtattta
     1141 gaacccttag ccgaaacctc gagatacaaa ccgccgccat cggccatttt gtaggttttc
     1201 tcttttggct ttgctgtctc gacctgtctg gcgttgagct tcatttgggg gcacatttct
     1261 aatcgaagtt aagatgcccc caattatgcc cccaatagca tccggatttc aacggactag
     1321 ctcggacagc tcaggacaga aaaaccgcta caggcattga ttttaaaggg tttgctggat
     1381 tttctcggat ggtcttggaa gtactaatgg tgccgaaggc cggactcgaa ccggcacgta
     1441 tttctacggt tgattttgaa tcaactgcgt ctaccgattt cgccacttcg gcactgaaga
     1501 ggtatgcgga aaacgttgtg gattatacct gtcgcacgcc accatgcaag cgccagcttg
     1561 cacccacctc gctaagtgct gaaattttca gcattacact cagcgatatg taacctttgt
     1621 cacactccag gcaccccgcc ctgccatgct ctacacttcc caaacaacac cagagaagga
     1681 cccaaaaatg tcgatgataa aaagctatgc cgcaaaagaa gcgggcggcg aactggaagt
     1741 ttatgagtac gatcccggtg agctgaagcc acaagatgtt gaagtgcagg tggattactg
     1801 cgggatttgt cattccgatc tgtcgatgat cgataacgaa tggggatttt cacaatatcc
     1861 gctggttgcc gggcatgagg tgattgggcg cgtggtggca ctcgggagcg ccgcgcagga
     1921 taaaggtttg caggtcggtc agcgtgtcgg gattggctgg acggcgcgta gctgtggtca
     1981 ctgcgacacc tgtattagcg gtaatcagat caactgcgag caaggtgcgg tgccgacgat
     2041 tatgaatcgc ggtggctttg ccgagaagtt gcgtgcggac tggcaatggg tgattccact
     2101 gccagaaaat attgatatcg agtccgccgg gccgctgttg tgcggcggta tcacggtctt
     2161 taaaccactg ttgatgcacc atatcactgc taccagccgc gttggggtaa ttggtattgg
     2221 cgggctgggg catatcgcta taaaacttct gcacgcaatg ggatgcgagg tgacggcctt
     2281 tagttctaat ccggcgaaag agcaggaagt actggcgatg ggtaccgata aagtggtgaa
     2341 tagccgcgat ccgcaggcac tgaaagccct gtcggggcag tttgatctca ttatcaatac
     2401 tgtgaacgtc agcctcgact ggcagcctta ttttgaggcg ctgacctatg gcggtaattt
     2461 ccatacggtc ggtgcggttc tcacgccgct gtctgttccg gcctttacgt taattgcggg
     2521 cgaccgcagc atctctggtt ctgctaccgg cacgccttat gagctgcgta agctgatgcg
     2581 ttttgccgcc cgcagcaagg ttgcgccgac caccgaactg ttcccgatgt cgaaaattaa
     2641 cgacgccatc cagcatgtac gcgatggtaa agcgcgctac cgcgtggtcc tgaaagccga
     2701 cttctgatcc ctttgccggg agcctgtcgc ccggcactta aatctctttg gaaggcgtct
     2761 cgcaatctgc tgaatttagc ggtgaagagt gcacagccgt tgcctatact ccaggcagga
     2821 aactggagga aatctcatga gcgaacccct gttaattgcc cgcacgccgg acacagaact
     2881 gtttttactg ccgggaatgg ctaaccgtca cgggctgatt actggcgcaa cggggacggg
     2941 taaaactgtc acgctgcaaa aactggcgga gtctctatcg gaaatcggcg tgccggtgtt
     3001 tatggccgat gtgaaaggcg atctgaccgg tatcgcgcag gcaggaacgg cgtcggaaaa
     3061 actgctcgca aggcttaaaa atatcggcgt aaatgactgg caaccgcatg ctaatccggt
     3121 ggtggtgtgg gatatctttg gcgagaaagg ccatccggtg cgggcgacgg tttcggatct
     3181 ggggccgctg ttgctggcgc ggctgttgaa tctcaacgat gtgcaatctg gcgtgctgaa
     3241 tatcatcttc cgaattgctg acgatcaggg gctgttactg ctcgacttta aagatctgcg
     3301 ggcgattacc cagtacatcg gcgataacgc caaatctttc cagaatcagt acggaaatat
     3361 cagtagcgca tcggttggtg ccatccagcg cgggctgttg tcgctggaac agcaaggcgc
     3421 agcacacttc tttggtgagc cgatgctgga tatcaaagac ttgatgcgca ccgatgccaa
     3481 cggtaaaggc gttattaata tcctcagcgc cgagaagctt tatcagatgc cgaaactgta
     3541 cgccgccagc ctgctgtgga tgatttcaga gttgtatgaa caattgccgg aagcgggcga
     3601 tctggagaag ccaaaactgg tgtttttctt cgacgaagca catctgctgt ttaacgatgc
     3661 accgcaggta ctgctggata agattgagca ggtgataagg cttattcgct caaaaggcgt
     3721 gggcgtctgg ttcgtttcgc aaaacccgtc tgatattccg gataatgtgc tcggccagct
     3781 cggtaatcgc gttcagcacg ctttgcgggc ttttacgcca aaagatcaga aagcggtaaa
     3841 agctgcggcg caaaccatgc gggccaatcc ggcgtttgat accgaaaagg caatccagga
     3901 actggggacc ggcgaggcgt taatctcgtt tctcgatgca aaaggaagtc cttctgtggt
     3961 ggaacgggcg atggtgattg cgccttgttc gcggatggga ccggtgacgg aagatgagcg
     4021 caatggcttg attaaccact ccccggtgta tggcaagtac gaggatgacg tggaccggga
     4081 atccgcctat gagatgttgc aaaaaggcgt acaggcgagt attgaacagc aaaataatcc
     4141 tccagcgaaa gggaaagaga tagcggtaga tgacggcatt cttggtggat tgaaggatat
     4201 tttgttcggc actaccggac cacgcggcgg gaagaaagat ggtgtggtgc agacaatggc
     4261 caaaagcgcc gctcgccagg tgacgaatca gattgtacgc gggatgttgg ggagtttgct
     4321 gggagggaga agaaggtaag tcaggcgcga tctgtccccc tcaccctaac cctctcccca
     4381 aaggggcgag gggaccgatc gaactcgtat ttgactttat caacaatgtg aaagggagct
     4441 ttcgctccct tgcttggtta cgattttctc attaacagcc acaggctgat taagaagaag
     4501 ctggcgcttg gcaacagtgc gccgataatc ggcgggatgc cgtaaaccaa cgtcagcggg
     4561 ccgaagatct ggtccagtac gtagaagaca aaaccgaagc tgataccggt gaccacacgc
     4621 acgcccatcg gtacgctacg cagtgggcca aagatgaacg acagcgccat cagcatcatc
     4681 accgccacgg atagcggctg gaagattttg ctccacatat tgagctgata acgtccggca
     4741 tcctgaccgc tcgacttcag atacttcacg tagttgtgca aaccgctgat tgagagtgca
     4801 tccgggtcca gcgccaccac gcccagtttg tctggcgtga ggttggtttt ccaggtgccg
     4861 ctcaccgtct gcgaaccggt gatctgtttc ggattggtca gatcagattc atcaacctgc
     4921 gacagacgcc agactttatg ttccgggtca aacttcgcag tagcggcata gcgtacggat
     4981 tgcagacgac gattttcgtt aaaggcataa atgctgatgc cacctaactc ttcgtcacct
     5041 ttaacccgct caatgtagac gaagttgttg ccatctttcg cccataagcc ttgctgggta
     5101 gagagcaacg agccgccgta catcgcctgc gcacggtagt tacgcgccat ctgctcgccc
     5161 tgcggcgcga cccattcgcc aatcgccatc gtcagcaaga ccagcggaat ggcggttttc
     5221 atcaccgaca gcgccacctg catacgggta aaaccagaag cctgcatcac caccagttcg
     5281 ctgcgctgcg ccagcatccc aagaccaagc aacgccccaa gcagagccgc catcgggaag
     5341 aagatttgca catctttcgg cacgctcagc aaggtataca ttcctgcgcc taacgcgtcg
     5401 taactcccct gcccggcttt tttcaactga tcgacaaatt tgataatgcc cgacagcgac
     5461 accagcatga acagcgtcat catgatggtg gtgaaaatag ttttaccgat atagcggtca
     5521 agtacgccaa aaggttgcat cacaccgctc ctttacgcga aaaactggcg cgcaggcggc
     5581 ggaccggcac ggtgtcccaa aggttgagaa caatcgccaa agccagataa atcaggttaa
     5641 cggtccacat ccacagcgtc gggtccagct tacctttacc gccgttcgat ttcagggagg
     5701 tctggatcag gaagaaaagt agatacagca gcatggctgg cagcatcgac agtacgcgtc
     5761 cctgacgtgg gttaaccacg ctcagcggta cgaccataag tgccatcata aacacggtga
     5821 ataccaacgt gatacgccag ttcagttctg cgcgagcacg atcggtgtca gtgttccaca
     5881 atgtgcacat gtccatctgg tcggtatcgt tcgggtcgag cgccaccgcc tggtgaccaa
     5941 tgatcgcctg ataatcctgg aaatccgtaa tgcggaaatc acgtaacagt gcagtgcctt
     6001 cgaagcgcgt tccctggttg agagtgacga cctgggagcc gtcgcgcagc tgggttaaat
     6061 gtccggaatc ggccaccacc acagaaggac gtgcattacc ttttggtcga atttgtgcga
     6121 ggaacacatc tttgaaatcg ctgccgtcaa cgctttcgat gaacagcacc gagctgccat
     6181 tagtcgcttg ctggaattgc ccttgcgcca gcgccgccat gccagggttc gctttcgctt
     6241 ctgctaacac ttcatcctga tgacgcgatg accacggtcc cgcccacatc acgttaaccg
     6301 ccgcgacgat tgccgtgaat accgcaagga tcattgccgc tttcaccaga accgctttgc
     6361 tcaggccgca ggcatgcatt accgtaattt cactttcggt atacagtttg cccagcgtca
     6421 tcagcagccc gaggaacagg cttaatggca ggataagctg cgccatttcc ggcacgccca
     6481 acccgagaag ggagagcacc agattcgccg gaatatcgcc gtcaaccgct gcgccgagga
     6541 tcctcactaa cttttgacag aagaagatca aaagcaagat gaagagtatc gccagctggc
     6601 ttttgagcgt ctcccgcacc agatatctta tgattatcac tttaaatacg cccgtaaaaa
     6661 ctcgtctttt gcaggatttt agcttgtttc atggcttaaa cgtcatttat tctcttgagt
     6721 cgtcgaaatc gtcgctaaga taattatact caacggattc acctctcaga ttttgttctg
     6781 acgtgccaat gccgtaataa cgttaagatt aacacgaagt catcgcaaca gcggacatga
     6841 gttacgaaag cttgcaattc tatctgtagc caccgccgtt gtctttaaga ttcaggagcg
     6901 tagtgcatgg agtttagtgt aaaaagcggt agcccggaga aacagcggag tgcctgcatc
     6961 gtcgtgggcg tcttcgaacc acgtcgcctt tctccgattg cagaacagct cgataaaatc
     7021 agcgatgggt acatcagcgc cctgctacgt cggggcgaac tggaaggaaa accggggcag
     7081 acattgttgc tgcaccatgt tccgaatgta ctttccgagc gaattctcct tattggttgc
     7141 ggcaaagaac gtgagctgga tgagcgtcag tacaagcagg ttattcagaa aaccattaat
     7201 acgctgaatg atactggctc aatggaagcg gtctgctttc tgactgaact gcacgttaaa
     7261 ggccgtaaca actactggaa agtgcgtcag gctgtcgaga cggcaaaaga aacgctctac
     7321 agtttcgatc agctgaaaac gaacaagagc gaaccgcgtc gtccgctgcg taaaatggtg
     7381 ttcaacgtgc cgacccgccg tgaactgacc agcggtgagc gcgcgatcca gcacggtctg
     7441 gcgattgccg ccgggattaa agcagcaaag gatcttggca atatgccgcc gaatatctgt
     7501 aacgccgctt acctcgcttc acaagcgcgc cagctggctg acagctacag caagaatgtt
     7561 atcacccgcg ttatcggcga acagcagatg aaagagctgg ggatgcattc ctatctggcg
     7621 gtcggtcagg gttcgcagaa cgaatcgctg atgtcggtga ttgagtacaa aggcaacgcg
     7681 tcggaagatg cacgcccaat cgtgctggtg ggtaaaggtt taaccttcga ctccggcggt
     7741 atctcgatca agccttcaga aggcatggat gagatgaagt acgatatgtg cggtgcggca
     7801 gcggtttacg gtgtgatgcg tatggtcgcg gagctacaac tgccgattaa cgttatcggc
     7861 gtgttggcag gctgcgaaaa catgcctggc ggacgagcct atcgtccggg cgatgtgtta
     7921 accaccatgt ccggtcaaac cgttgaagtg ctgaatactg acgctgaagg ccgcctggta
     7981 ctgtgcgacg tgttaactta cgttgagcgt tttgagccgg aagcggtgat tgacgtggcg
     8041 acgctgaccg gtgcctgcgt gatcgcgctg ggtcatcaca ttaccggtct gatggcgaac
     8101 cataatccgc tggcccatga actgattgcc gcgtctgaac aatccggtga ccgcgcatgg
     8161 cgcttaccgc tgggtgacga gtatcaggaa cagctggagt ccaattttgc cgatatggcg
     8221 aacattggcg gtcgtcctgg tggggcgatt accgcaggtt gcttcctgtc gcgctttacc
     8281 cgtaagtaca actgggcgca cctggatatc gccggaaccg cctggcgttc tggtaaagca
     8341 aaaggcgcaa ccggtcgtcc ggtagcgttg ctggcacagt tcctgttgaa ccgcgctggg
     8401 tttaacggcg aagagtaatt gcgtcaggca aggctgttat tgccggatgc ggcgtgaacg
     8461 ccttatccga cctacacagc actgaactcg taggcctgat aagacgcgca agcgtcgcat
     8521 caggcaatgc tgttattgtc ggatgcggca tgaacgcctt atccgaccta cacagcaccg
     8581 aactcgtagg cctgataaga cacaacagcg tcgcatcagg caatgctgtt attgtcggat
     8641 gcggcgtgag cgccttatcc gacctacaca cagcactgaa ctcgtaggcc tgataagaca
     8701 caacagcgtc gcatcaggcg ctgcggtgta tacctgatgc gtatttaaat ccaccacaag
     8761 aagccccatt tatgaaaaac gcgacgttct accttctgga caatgacacc accgtcgatg
     8821 gcttaagcgc cgttgagcaa ctggtgtgtg aaattgccgc agaacgttgg cgcagcggta
     8881 agcgcgtgct catcgcctgt gaagatgaaa agcaggctta ccggctggat gaagccctgt
     8941 gggcgcgtcc ggcagaaagc tttgttccgc ataatttagc gggagaagga ccgcgtggcg
     9001 gtgcgccggt ggagatcgcc tggccgcaaa aacgcagcag cagcccgcgc gatatattga
     9061 ttagtctgcg aacatgcttt gcagattttg ccaccgcttt cacagaagtg gtagacttcg
     9121 ttccttatga agattctctg aaacaactgg cgcgcgaacg ctataaagcc taccgcgtgg
     9181 ctggtttcaa cctgaatacg gcaacctgga aataatggaa aagacatata acccacaaga
     9241 tatcgaacag ccgctttacg agcactggga aaagcagggc tactttaagc ctaatggcga
     9301 tgaaagccag gaaagtttct gcatcatgat cccgccgccg aacgtcaccg gcagtttgca
     9361 tatgggtcac gccttccagc aaaccatcat ggataccatg atccgctatc agcgcatgca
     9421 gggcaaaaac accctgtggc aggtcggtac tgaccacgcc gggatcgcta cccagatggt
     9481 cgttgagcgc aagattgccg cagaagaagg taaaacccgt cacgactacg gccgcgaagc
     9541 tttcatcgac aaaatctggg aatggaaagc ggaatccggc ggcacgatta cccgtcagat
     9601 gcgccgtctc ggcaactccg tcgactggga gcgtgaacgc ttcacgatgg acgaaggcct
     9661 gtccaatgcg gtgaaagaag ttttcgttcg tctgtataaa gaagacctga tttaccgtgg
     9721 caaacgcctg gtaaactggg acccgaaact gcgcaccgct atctctgacc tggaagtgga
     9781 aaaccgcgaa tcgaaaggtt cgatgtggca catccgctat ccgctggctg acggtgcgaa
     9841 aaccgcagac ggtaaagatt acctggtggt tgcgactacc cgtccggaaa ccctgctggg
     9901 cgataccggt gtagccgtta acccggaaga tccgcgttac aaagacctga ttggcaaata
     9961 tgtcattcta ccgctggtta accgtcgtat tccgatcgtt ggcgacgaac acgccgacat
    10021 ggaaaaaggc accggctgcg tgaagatcac cccggcgcac gactttaacg actatgaagt
    10081 cggtaaacgt cacgccctgc cgatgatcaa catcctgacc tttgacggcg atatccgtga
    10141 aagcgcccag gtgttcgata ccaaaggtaa cgaatctgac gtttattcca gcgagatccc
    10201 ggcagaattc cagaaactgg agcgttttgc cgcacgtaaa gcagtcgttg ctgcggttga
    10261 cgcactcggc ctgctggaag aaattaaacc gcacgacctg accgttcctt acggcgaccg
    10321 tggcggcgta gttatcgaac caatgctgac cgaccagtgg tacgtgcgtg ccgatgtact
    10381 ggcgaaaccg gcggttgaag cggttgagaa cggcgacatt cagttcgtac cgaagcagta
    10441 cgaaaacatg tatttctcct ggatgcgcga tattcaggac tggtgtatct ctcgtcagct
    10501 gtggtggggt caccgtatcc cggcatggta tgacgaagcg ggtaatgttt atgttggccg
    10561 caacgaagaa gaagtgcgca aggaaaataa cctcggtgcc gacgttgccc tgcgtcagga
    10621 cgaagacgtt ctcgacacct ggttctcttc tgcgctgtgg accttctcta cccttggctg
    10681 gccggaaaac accgacgccc tgcgtcagtt ccacccaacc agcgtgatgg tatccggctt
    10741 cgacatcatc ttcttctgga ttgcccgcat gatcatgatg accatgcact tcatcaaaga
    10801 tgaaaatggc aaaccgcagg tgccgttcca cactgtttac atgaccggtc tgattcgtga
    10861 cgacgaaggc cagaaaatgt ccaaatccaa gggtaacgtt atcgacccgc tggatatggt
    10921 tgacggtatt tcgctgccag aactgctgga aaaacgtacc ggcaatatga tgcagccgca
    10981 gctggcggac aaaatccgta agcgcaccga gaaacagttc ccgaacggca ttgagccgca
    11041 cggcaccgat gccctgcgct tcaccctggc ggcgctggcg tctaccggtc gtgacatcaa
    11101 ctgggatatg aagcgcctgg aaggttaccg taacttctgt aacaagctgt ggaacgccag
    11161 ccgctttgtg ctgatgaaca cagaaggtca ggattgcggc tttaacggcg gcgaaatgac
    11221 actgtcgctg gcggaccgct ggattctggc agagttcaac cagaccatca aagcgtaccg
    11281 cgaagcgctg gacagcttcc gcttcgatat cgctgcaggc attctgtatg agttcacctg
    11341 gaaccagttc tgtgactggt atctggagct gaccaagccg gtaatgaacg gtggcaccga
    11401 agccgaactg cgcggtactc gccatacgct ggtgactgta ctggaaggtc tgctgcgcct
    11461 cgcgcatccg atcattccgt tcatcaccga aaccatctgg cagcgtgtga aagtactttg
    11521 cggtatcact gccgacacca tcatgctgca gccgttcccg cagtacgatg catctcaggt
    11581 tgatgaagcc gcactggccg acaccgaatg gctgaaacag gcgatcgttg cggtacgtaa
    11641 catccgtgca gaaatgaaca tcgcgccggg caaaccgctg gagctgctgc tgcgtggttg
    11701 cagcgcggat gcagaacgtc gcgtaaatga aaaccgtggc ttcctgcaaa ccctggcgcg
    11761 tctggaaagc atcaccgtgc tgcctgccga tgacaaaggt ccggtttccg ttacgaagat
    11821 catcgacggc gcagagctgc tgatcccgat ggctggcctc atcaacaaag aagatgagct
    11881 ggcgcgtctg gcgaaagaag tggcgaagat cgaaggcgaa atcagccgta tcgagaacaa
    11941 gctggcgaat gaaggctttg tcgcccgcgc accggaagcg gtcatcgcga aagagcgtga
    12001 gaagctggaa ggctatgcgg aagcgaaagc gaagctgatt gaacagcagg ctgttatcgc
    12061 cgcgctgtaa tcaacaccgt gatgaaaacg aaggccggag aatgctccgg ccttttttat
    12121 ctcttacaga aatggtaaag aaggcataat tccccgtgaa atactggcta agaagccttt
    12181 atctggttgt tcttctttat tgattaaagg aaggtcatcc aaatcgccca gcaaatacgt
    12241 attttttgcc tgccagtcaa tcatccatat tttcagcagt ggataagcca gaccgcccgt
    12301 aatcccggat atcactacca acgcacacat gcgataaagc ataccgtggt aagttaaagt
    12361 tgagcgaaaa cgaatacgcc catcattcag tgacaggttg ctcataaaat gatttcgcaa
    12421 cgacaccgtt aaataactgg tgctaacagc aatcccaaaa taatagatta actgcgcgat
    12481 tatcatttta cgttgcattt ccataaattg ctgtaaattc tcaatatcat catttgcata
    12541 cacagaacta tcatacgcat ttaatatttg atcgaagata atataaccag caacagcgag
    12601 aaaaggtaat aaagctaaaa atgcgaggat ggcatatttt atacaatacg cagtatctaa
    12661 tttcaccttg aaacgatgta taccgaaact ggtattgctc cagagaaaac tcattaccag
    12721 gctatataaa gtaccattaa aaataccaat ggaaacaata ccaactattg ccatcagaac
    12781 cacagatata ataacactac ttgaactatt ggcatgtagc atctttgtcg agataaagaa
    12841 aacagtcccc atcccaatgg ccattaaaat cggcaagaaa aaggttactc accagaaccc
    12901 tttcatagag caattaaaac taaatcttac gccgttgaga ctagtcatca aggcctgata
    12961 acgcagtcct tttgctgcca taaatataag caaaaccaac agtgacaaag tcaaaacaca
    13021 gccaataagc ggcatatctg ctgacactgt cattaagatt gcgaaataga aaaaaacaaa
    13081 aacaagacaa ctaacaaaaa cattcccacc ggtaatccca taagaaaatc gttgtccgtt
    13141 aacctccata ttagcataga gataacgctt acatttcatt aatgcccatg gtaaatagat
    13201 acctagcgtt ataatcgtta acaacacatt caccacacaa ataagaaaat atctctcacc
    13261 tgtaccatga aaaacaaacg aatgggacgg aacatctatt tcattaata
//
LOCUS       DAZKHF020000119        13155 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2201S31241
            SAMN43267800-rid25106413.denovo.120, whole genome shotgun sequence.
ACCESSION   DAZKHF020000119 DAZKHF020000000
VERSION     DAZKHF020000119.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267800
            Sequence Read Archive: SRR30307263
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 13155)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13155)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 13155)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350581.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350581.2
            Genome Coverage       :: 151x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:21:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,652
            CDSs (total)                      :: 5,557
            Genes (coding)                    :: 5,320
            CDSs (with protein)               :: 5,320
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 237
            CDSs (without protein)            :: 237
            Pseudo Genes (ambiguous residues) :: 0 of 237
            Pseudo Genes (frameshifted)       :: 102 of 237
            Pseudo Genes (incomplete)         :: 130 of 237
            Pseudo Genes (internal stop)      :: 52 of 237
            Pseudo Genes (multiple problems)  :: 41 of 237
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13155
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267800-rid25106413.denovo.120"
                     /serotype="O 26:H11"
                     /isolate="2201S31241"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-01"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            <1..65
                     /locus_tag="ACHOSC_RS13935"
                     /old_locus_tag="pgaptmp_002787"
     CDS             <1..65
                     /locus_tag="ACHOSC_RS13935"
                     /old_locus_tag="pgaptmp_002787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416352.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4548695.1"
                     /translation="LHYIDTGAVFGGELTLVQLQ"
     gene            complement(66..257)
                     /gene="yebW"
                     /locus_tag="ACHOSC_RS13940"
                     /old_locus_tag="pgaptmp_002788"
     CDS             complement(66..257)
                     /gene="yebW"
                     /locus_tag="ACHOSC_RS13940"
                     /old_locus_tag="pgaptmp_002788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416351.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebW family protein"
                     /protein_id="HGU4548696.1"
                     /translation="MFALVLFVCYLDGGCEDIVVDVYNTEQQCLYSMSDQRIRHGGCF
                     PIEDFIDGFWRPAQEYGDF"
     gene            complement(362..598)
                     /gene="yebV"
                     /locus_tag="ACHOSC_RS13945"
                     /old_locus_tag="pgaptmp_002789"
     CDS             complement(362..598)
                     /gene="yebV"
                     /locus_tag="ACHOSC_RS13945"
                     /old_locus_tag="pgaptmp_002789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707284.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebV family protein"
                     /protein_id="HGU4548697.1"
                     /translation="MKTSVRIGAFEIDDGELHGESPGDRTLTIPCKSDPDLCMQLDAW
                     DAETSIPALLNGEHSVLYRTRYDQQSDAWIMRLA"
     gene            complement(716..2155)
                     /gene="rsmF"
                     /locus_tag="ACHOSC_RS13950"
                     /old_locus_tag="pgaptmp_002790"
     CDS             complement(716..2155)
                     /gene="rsmF"
                     /locus_tag="ACHOSC_RS13950"
                     /old_locus_tag="pgaptmp_002790"
                     /EC_number="2.1.1.178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416349.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0008649 - rRNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0001510 - RNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(1407)-C(5))-methyltransferase
                     RsmF"
                     /protein_id="HGU4548698.1"
                     /translation="MAQHTVYFPDAFLTQMREAMPSTLSFDDFLAACQRPLRRSIRVN
                     TLKISVADFLQLTAPYGWTLTPIPWCEEGFWIERDNEDALPLGSTAEHLSGLFYIQEA
                     SSMLPVAALFADDNAPQRVMDVAAAPGSKTTQIAARMNNEGAILANEFSASRVKVLHA
                     NISRCGISNVALTHFDGRVFGAAVPEMFDAILLDAPCSGEGVVRKDPDALKNWSPESN
                     QEIAATQRELIDSAFHALRPGGTLVYSTCTLNQEENEAVCLWLKETYPDAVEFLPLGD
                     LFPGANKALTEEGFLHVFPQIYDCEGFFVARLRKTQAIPALPAPKYKVGNFPFSPVKD
                     REAGQIRQATAGVGLNWDENLRLWQRDKELWLFPVGIEALIGKVRFSRLGIKLAETHN
                     KGYRWQHEAVIALASPDNMNAFELTPQEAEEWYRGRDVYPQAAPVADDVLVTFQHQPI
                     GLAKRIGSRLKNSYPRELVRDGKLFTGNA"
     gene            complement(2235..4868)
                     /gene="yebT"
                     /locus_tag="ACHOSC_RS13955"
                     /old_locus_tag="pgaptmp_002791"
     CDS             complement(2235..4868)
                     /gene="yebT"
                     /locus_tag="ACHOSC_RS13955"
                     /old_locus_tag="pgaptmp_002791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416348.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid-binding membrane homeostasis protein YebT"
                     /protein_id="HGU4548699.1"
                     /translation="MSQETPASTTEAQIKNKRRISPFWLLPFIALMIAGWLIWDSYQD
                     RGNTVTIDFMSADGIVPGRTPVRYQGVEVGTVQDISLSDDLRKIEVKVSIKSDMKDAL
                     REETQFWLVTPKASLAGVSGLDALVGGNYIGMMPGKGKEQDHFVALDTQPKYRLDNGD
                     LMIHLQAPDLGSLNSGSLVYFRKIPVGKVYDYAINPNKQGVVIDVLIERRFTDLVKKG
                     SRFWNVSGVDANVSVSGAKVKLESLAALVNGAIAFDSPEESKPAEAEDTFGLYEDLAH
                     SQRGVIIKLELPSGAGLTADSTPLMYQGLEVGQLTKLDLNPGGKVTGEMTVDPSVVTL
                     LRENTRIELRNPKLSLSDANLSALLTGKTFELVPGDGEPRKEFVVVPGEKALLHEPDV
                     LTLTLTAPESYGIDAGQPLILHGVQVGQVIDRKLTSKGVTFTVAIEPQHRELVKGDSK
                     FVVNSRVDVKVGLDGVEFLGASASEWINGGIRILPGDKGEMKASYPLYANLEKALENS
                     LSDLPTTTVSLSAETLPDVQAGSVVLYRKFEVGEVITVRPRANAFDIDLHIKPEYRNL
                     LTSNSVFWAEGGAKVQLNGSGLTVQASPLSRALKGAISFDNLSGASASQRKGDKRILY
                     ASETAARAVGGQITLHAFDAGKLAVGMPIRYLGIDIGQIQTLDLITARNEVQAKAVLY
                     PEYVQTFARGGTRFSVVTPQISAAGVEHLDTILQPYINVEPGRGNPRRDFELQEATIT
                     DSRYLDGLSIIVEAPEAGSLGIGTPVLFRGLEVGTVTGMTLGTLSDRVMIAMRISKRY
                     QHLVRNNSVFWLASGYSLDFGLTGGVVKTGTFNQFIRGGIAFATPPGTPLAPKAQEGK
                     HFLLQESEPKEWREWGTALPK"
     gene            complement(4837..6120)
                     /gene="yebS"
                     /locus_tag="ACHOSC_RS13960"
                     /old_locus_tag="pgaptmp_002792"
     CDS             complement(4837..6120)
                     /gene="yebS"
                     /locus_tag="ACHOSC_RS13960"
                     /old_locus_tag="pgaptmp_002792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane integrity lipid transport subunit YebS"
                     /protein_id="HGU4548700.1"
                     /translation="MALNTPQITPTKKITVRAIGEELPRGDYQRCPQCDMLFSLPEIN
                     SHQSAYCPRCQAKIRDGRDWSLTRLAAMAFTMLLLMPFAWGEPLLHIWLLGIRIDANV
                     MQGIWQMTKQGDTITGAMVFFCVIGAPLILVSSIAYLWFGNRLGMNLRPVLLMLERLK
                     EWVMLDIYLVGIGVASIKVQDYAHIQAGVGLFSFVALVILTTVTLSHLNVEELWERYY
                     PQRPATRRDEKLRVCLGCHFTGYPDQRGRCPRCHIPLRLRRRHSLQKCWAALLASIVL
                     LLPANLLPISIIYLNGGRQEDTILSGIMSLASSNIAVAGIVFIASILVPFTKVIVMFT
                     LLLSIHFKCQQGLRTRILLLRMVTWIGRWSMLDLFVISLTMSLINRDQILAFTMGPAA
                     FYFGAAVILTILAVEWLDSRLLWDAHESGNARFDD"
     gene            6250..6747
                     /gene="msrC"
                     /locus_tag="ACHOSC_RS13965"
                     /old_locus_tag="pgaptmp_002793"
     CDS             6250..6747
                     /gene="msrC"
                     /locus_tag="ACHOSC_RS13965"
                     /old_locus_tag="pgaptmp_002793"
                     /EC_number="1.8.4.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-methionine (R)-S-oxide reductase"
                     /protein_id="HGU4548701.1"
                     /translation="MNKTEFYADLNRDFNALMAGETSFLATLANTSALLYERLTDVNW
                     AGFYLLEDDTLVLGPFQGKIACVRIPVGRGVCGTAVARNQVQRIEDVHAFDGHIACDA
                     ASNSEIVLPLVVKNQIIGVLDIDSTVFGRFTDEDEQGLRQLVAQLEKVLATTDYKKFF
                     ASVAG"
     gene            6844..7542
                     /gene="proQ"
                     /locus_tag="ACHOSC_RS13970"
                     /old_locus_tag="pgaptmp_002794"
     CDS             6844..7542
                     /gene="proQ"
                     /locus_tag="ACHOSC_RS13970"
                     /old_locus_tag="pgaptmp_002794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707288.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0033592 - RNA strand annealing activity
                     [Evidence IEA];
                     GO_function: GO:0034057 - RNA strand-exchange activity
                     [Evidence IEA];
                     GO_process: GO:0010608 - post-transcriptional regulation
                     of gene expression [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA chaperone ProQ"
                     /protein_id="HGU4548702.1"
                     /translation="MENQPKLNSSKEVIAFLAERFPHCFSAEGEARPLKIGIFQDLVD
                     RVAGEMNLSKTQLRSALRLYTSSWRYLYGVKPGATRVDLDGNPCGELDEQHVEHARKQ
                     LEEAKARVQAQRAEQQAKKREAAAAAGEKEDAPRRERKPRPTTPRRKEGAERKPRAQK
                     PVEKAPKTVKAPREEQHTPVSDISALTVGQALKVKAGQNAMDATVLEITKDGVRVQLN
                     SGMSLIVRAEHLVF"
     gene            7562..9610
                     /gene="prc"
                     /locus_tag="ACHOSC_RS13975"
                     /old_locus_tag="pgaptmp_002795"
     CDS             7562..9610
                     /gene="prc"
                     /locus_tag="ACHOSC_RS13975"
                     /old_locus_tag="pgaptmp_002795"
                     /EC_number="3.4.21.102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxy terminal-processing peptidase"
                     /protein_id="HGU4548703.1"
                     /translation="MNMFFRLTALAGLLAIAGQTFAVEDITRADQIPVLKEETQHATV
                     SERVTSRFTRSHYRQFDLDQAFSAKIFDRYLNLLDYSHNVLLASDVEQFAKKKTELGD
                     ELRSGKLDVFYDLYNLAQKRRFERYQYALSVLEKPMDFTGNDTYNLDRSKAPWPKNEA
                     ELNALWDSKVKFDELSLKLAGKTDKEIRETLTRRYKFAIRRLAQTNSEDVFSLAMTAF
                     AREIDPHTNYLSPRNTEQFNTEMSLSLEGIGAVLQMDDDYTVINSMVAGGPAAKSKAI
                     SVGDKIVGVGQTGKPMVDVIGWRLDDVVALIKGPKGSKVRLEILPAGKGTKTRTVTLT
                     RERIRLEDRAVKMSVKTVGKEKVGVLDIPGFYVGLTDDVKVQLQKLEKQNVSSVIIDL
                     RSNGGGALTEAVSLSGLFIPAGPIVQVRDNNGKVREDSDTDGQVFYKGPLVVLVDRFS
                     ASASEIFAAAMQDYGRALVVGEPTFGKGTVQQYRSLNRIYDQMLRPEWPALGSVQYTI
                     QKFYRVNGGSTQRKGVTPDIIMPTGNEETETGEKFEDNALPWDSIDAATYVKSGDLTA
                     FGPELLKEHNARIAKDPEFQNIMKDIARFNAMKDKRNIVSLNYAVREKENNEDDATRL
                     ARLNERFKREGKPELKKLDDLPKDYQEPDPYLDETVNIALDLAKLEKARPAEQPDPVK
                     "
     gene            9802..10683
                     /gene="htpX"
                     /locus_tag="ACHOSC_RS13980"
                     /old_locus_tag="pgaptmp_002796"
     CDS             9802..10683
                     /gene="htpX"
                     /locus_tag="ACHOSC_RS13980"
                     /old_locus_tag="pgaptmp_002796"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014229823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protease HtpX"
                     /protein_id="HGU4548704.1"
                     /translation="MMRIALFLLTNLAVMVVFGLVLSLTGIQSSSVQGLMIMALLFGF
                     GGSFVSLLMSKWMALRSVGGEVIEQPRNERERWLVNTVATQARQAGIAMPQVAIYHAP
                     DINAFATGARRDASLVAVSTGLLQNMSPDEAEAVIAHEISHIANGDMVTMTLIQGVVN
                     TFVIFISRILAQLAAGFMGGNRDEGEESNGNPLIYFAVATVLELVFGILASIITMWFS
                     RHREFHADAGSAKLVGREKMIAALQRLKTSYEPQEATSMMAFCINGKSKSLSELFMTH
                     PPLDKRIEALRTGEYLK"
     gene            complement(10729..12102)
                     /gene="yebQ"
                     /locus_tag="ACHOSC_RS13985"
                     /old_locus_tag="pgaptmp_002797"
     CDS             complement(10729..12102)
                     /gene="yebQ"
                     /locus_tag="ACHOSC_RS13985"
                     /old_locus_tag="pgaptmp_002797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416342.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HGU4548705.1"
                     /translation="MPKVQADGLPLPQRYGAILTIVIGISMAVLDGAIANVALPTIAT
                     DLHATPARSIWVVNAYQIAIVISLLSFSFLGDMFGYRRIYKCGLVVFLLSSLFCALSD
                     SLQMLTLARVIQGFGGAALMSVNTALIRLIYPQRFLGRGMGINSFIVAVSSAAGPTIA
                     AAILSIASWKWLFLINVPLGIIALLLAMRFLPPNGSRASKPRFDLPSAVMNALTFGLL
                     ITALSGFAQGQSLTLIAAELVVMVVVGIFFIRRQLSLPVPLLPVDLLRIPLFSLSICT
                     SVCSFCAQMLAMVSLPFYLQTVLGRSEVETGLLLTPWPLATMVMAPLAGYLIERVHAG
                     LLGALGLFIMAAGLFSLVLLPASPADINIIWPMILCGAGFGLFQSPNNHTIITSAPRE
                     RSGGASGMLGTARLLGQSSGAALVALMLNQFGDNGTHVSLMAAAILAVIAACVSGLRI
                     TQPRSRA"
     gene            12279..13070
                     /gene="kdgR"
                     /locus_tag="ACHOSC_RS13990"
                     /old_locus_tag="pgaptmp_002798"
     CDS             12279..13070
                     /gene="kdgR"
                     /locus_tag="ACHOSC_RS13990"
                     /old_locus_tag="pgaptmp_002798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator KdgR"
                     /protein_id="HGU4548706.1"
                     /translation="MANADLDKQPDSVSSVLKVFGILQALGEEREIGITELSQRVMMS
                     KSTVYRFLQTMKTLGYVAQEGESEKYSLTLKLFELGARALQNVDLIRSADIQMREISR
                     LTKETIHLGALDEDSIVYIHKIDSMYNLRMYSRIGRRNPLYSTAIGKVLLAWRDLDEV
                     KQILEGVEYKRSTERTITSTEALLPVLDQVREQGYGEDNEEQEEGLRCIAVPVFDRFG
                     VVIAGLSISFPTLRFSEERLQEYVAMLHTAARKISAQMGYHDYPF"
BASE COUNT         3446 a         3283 c         3414 g         3012 t
ORIGIN      
        1 acctgcatta tattgatacc ggtgctgtct ttgggggcga actgactctt gtgcaattgc
       61 aataattaaa aatcaccata ctcctgtgct ggtcgccaga aaccatctat aaaatcctca
      121 atcggaaaac aaccgccatg gcggatccgt tgatcgctca tagaataaag acactgctgt
      181 tccgtgttgt agacatccac aacaatatct tcacaaccgc catccaggta gcaaacaaaa
      241 agtaccagcg cgaacatttc atccccgaag tgtggtgccg taccgttaag tttaggagag
      301 attttacaac gggggaataa ccaggacaaa taacccgcca tgccgacggg ttctttttgg
      361 atcaggcaag acgcataatc caggcatcag attgttgatc gtaacgggta cggtaaagga
      421 cagagtgttc gccatttagc agggcaggga tactggtttc agcatcccag gcatcgagtt
      481 gcatacataa atcggggtca gatttacaag gaatggttaa cgttcgatca cccggcgatt
      541 caccgtgtaa ttcgccgtcg tcgatttcaa aagcgcctat gcgcacactg gttttcatct
      601 tcgtgctctc catttagctg ccgattgtgg tacgaccagt ccgatcgcac catttttcag
      661 cctagttgag gcagccgaat gtgccagtca aaaagtgcgc tttttttgtg agctgtcagg
      721 cgttaccggt aaaaagtttc ccatcgcgca ccagttcacg cggatagctg tttttcaatc
      781 gcgaaccaat ccgtttggct aaaccaatcg gttgatgctg gaaagtaacc aatacatcat
      841 ccgccactgg cgcggcttgc gggtaaacat cgcgcccgcg ataccactcc tccgcttcct
      901 gcggtgtcag ctcgaatgca ttcatattat cgggtgaggc aagagcaata accgcttcat
      961 gctgccagcg ataacctttg ttgtgcgtct cggcaagttt aatccccaac cgagaaaatc
     1021 ggactttacc gatcagggct tcaatgccca ccgggaacaa ccacagttct ttgtcacgct
     1081 gccagaggcg caggttttca tcccagttta agccaacacc tgcagtcgcc tgacgaattt
     1141 gtccagcttc gcgatctttc accgggctga acggaaaatt accgactttg tatttggggg
     1201 cgggtaaggc gggaatcgcc tgagttttac gcagacgagc aacgaagaag ccttcgcagt
     1261 cgtaaatttg tgggaaaaca tgcaaaaagc cttcttcggt cagcgctttg tttgcaccag
     1321 ggaagagatc gccaagtggt aaaaactcta ctgcgtcggg gtaagtctct ttcagccaca
     1381 ggcaaacggc ttcgttttct tcctggttta aggtacaggt cgagtaaacc agcgtaccac
     1441 caggacgtaa tgcatgaaag gcgctgtcga taagctcccg ttgtgtagct gcgatttcct
     1501 gattgctttc tggtgaccag ttttttagcg catcgggatc tttacgcacc acgccttcgc
     1561 cagagcaggg agcgtccagc aaaatggcat cgaacatttc tggcactgcc gcaccaaaca
     1621 cgcggccatc aaaatgtgtg agcgcaacat tactgatgcc acagcggctg atattggcat
     1681 gtaacacttt tacccgactg gcggaaaact cattggcaag gattgcccct tcgttattca
     1741 tccgcgcggc aatttgcgtc gttttggagc ctggcgcagc tgcgacatcc atcacccgct
     1801 gtggtgcatt atcgtcagca aacaaggcgg caacgggcaa cattgaactg gcttcctgaa
     1861 tataaaacag gccgcttaaa tgctcggcgg tactacccaa tggcaatgca tcttcattgt
     1921 cgcgttcaat ccagaaacct tcttcacacc acggaattgg cgtaagcgtc cagccataag
     1981 gagcggttaa ttgcaggaaa tcagcaacgg agattttcag cgtattaacg cgaatgctgc
     2041 ggcgcaacgg gcgctgacag gcggcaagaa aatcatcaaa tgagagcgtc gaaggcatcg
     2101 cttcgcgcat ttgtgtcaga aaggcgtccg ggaaataaac ggtgtgttgg gccacgagca
     2161 tgtaccactg gcaaaaaaac aggcgcgcag tttagcataa acgctccggc gcaggcacgc
     2221 cggagcagtg ggcattattt gggaagcgca gttccccatt cacgccactc tttcggttca
     2281 ctttcctgca acaggaagtg tttgccttcc tgggctttcg gtgccagtgg cgtacccgga
     2341 ggcgtggcga aggcgatgcc gccacggata aactggttaa aggtgccggt tttcactacg
     2401 ccgcccgtca gaccaaagtc cagactgtaa cccgatgcca accagaagac ggaattgtta
     2461 cgcaccaggt gttgatagcg tttactgatg cgcatcgcaa tcatcacgcg atctgacaat
     2521 gtccccagcg tcattcctgt aaccgtaccg acttccagac cacggaacag cacaggcgta
     2581 ccaataccta acgaaccggc ttccggcgct tcaacaataa tgcttaagcc atccaggtaa
     2641 cgcgaatcag taatggtggc ctcttgtaat tcaaagtcgc ggcgaggatt gccccggcct
     2701 ggttcgacgt tgatatacgg ctggaggata gtatctagat gctcaacgcc cgcggctgaa
     2761 atttgcggtg tgaccaccga gaagcgcgta ccgccgcggg cgaaggtctg gacatactcc
     2821 ggatagagca ccgcctttgc ctgtacttca ttgcgcgcgg taatcagatc cagcgtctgg
     2881 atttgcccga tatcaatacc gagatagcga attggcatcc cgaccgccag ttttccggca
     2941 tcgaaagcgt gaagcgtaat ctgcccacca accgcacggg ccgctgtttc ggaagcatac
     3001 agaattcgtt tgtcgccttt acgctgactg gcgctggcac cgctgaggtt atcgaagcta
     3061 atggctccct ttaatgctct ggagagcggg gatgcctgta cggtcaggcc actaccattc
     3121 agctgaactt tcgccccgcc ttctgcccag aacacgctat tgctggtcag aaggttgcga
     3181 tactccggct taatatgcag atcgatatca aacgcgttag ctcgcggacg gacggtaata
     3241 acttcaccaa cttcaaattt acggtagagc actaccgatc ctgcctgcac atccggcagc
     3301 gtctctgcac tcaaactcac ggttgtggtg ggtaaatcgc taaggctgtt ctccagcgct
     3361 ttttccagat tggcatacag tggatagctg gctttcatct cgcctttatc gcccggcaga
     3421 atacgtatcc cgccgttaat ccattctgag gcgctggcac cgagaaactc aacgccatcc
     3481 agccccacct tcacgtcgac acggctgttg acgacaaatt tgctatcgcc ttttaccagt
     3541 tctcgatgct gaggctcgat ggcgacggta aaggtgacgc ctttgctggt gagtttacga
     3601 tcgataacct ggcctacctg cacgccgtga agaatgagcg gctgaccagc atcaataccg
     3661 taactttccg gcgcggtcag ggtcagcgtc agaacatcag gttcatgcag cagtgctttt
     3721 tcgcctggca caacaacgaa ctctttgcgt ggctcgccat cgccgggtac caactcgaag
     3781 gttttgccag tcagcagggc gctgagattg gcatcgctaa gggataattt cggattacgt
     3841 aattcgatgc gggtattttc acgaagcagg gtaacgacgc tgggatcaac tgtcatttcc
     3901 ccagtgactt taccaccagg atttaaatcc agtttagtta gctgtccgac ttccagcccc
     3961 tgatacatta acggcgtcga gtcggcggtc aatccggccc cactcggcag ttccagtttt
     4021 attattacgc cacgctggct gtgggccaga tcttcataca gaccaaaggt atcttccgcc
     4081 tcggcaggtt tcgactcttc aggtgaatcg aaggcaatcg caccgttaac cagtgccgcc
     4141 agactttcca gtttcacctt cgcgccactg acactgacgt tggcatcaac gccggaaacg
     4201 ttccagaaac ggctaccttt tttcaccaga tcggtaaaac gccgctcgat caggacatca
     4261 atcaccacgc cttgcttgtt gggattgatg gcatagtcgt agacttttcc caccgggatc
     4321 ttgcggaaat agaccaatga accgctgttc agcgaaccga gatcgggagc ttgcaggtgg
     4381 atcatcagat cgccgttatc cagccgatac ttcggttggg tatcgagtgc gacaaagtga
     4441 tcctgctctt tacctttacc cggcatcatg ccgatatagt tcccaccgac gagggcgtcc
     4501 agcccggaga cacctgccaa cgatgctttt ggcgtcacca gccagaattg agtctcttcg
     4561 cgcagcgcat ctttcatatc ggacttgatg ctgaccttga cttcaatctt acgaagatcg
     4621 tcgctgaggc tgatatcctg cactgttccg acttcaacgc cctgataacg aacaggcgta
     4681 cggcccggaa caataccatc cgccgacata aagtcgatgg tgacggtatt accccggtcc
     4741 tgataactgt cccaaatcag ccaaccggca atcattagcg cgatgaaagg cagcagccag
     4801 aaaggtgaga tacggcgttt atttttaatc tgcgcttcag tcgtcgaagc gggcgtttcc
     4861 tgactcatgt gcatcccaaa gtaagcggct gtccagccat tccacagcaa gaatagtcaa
     4921 aattacggct gcgccaaaat aaaacgcagc cggtcccata gtaaaagcga ggatctgatc
     4981 gcgattaatc agcgacatgg ttaaagatat gacaaacagg tcgagcatcg accagcgtcc
     5041 aatccaggtc accatccgca gtaacagaat gcgtgtgcgt aaaccttgct ggcatttaaa
     5101 atgaatgctc aacagtaaag tgaacatgac gatcacttta gtaaacggta ccagaatact
     5161 ggcgataaac acgattcccg caaccgcaat gttgctactt gccagcgaca taattccgga
     5221 aagaattgta tcttcctgcc gtcctccgtt cagataaatg atagaaatag gcaataggtt
     5281 ggctggtaac aacaaaacga ttgacgctaa cagcgccgcc cagcattttt gcagactatg
     5341 acggcgacgc aggcgtagcg ggatatggca acgcgggcag cgaccacgct gatcaggata
     5401 gccggtaaaa tggcacccaa gacagacacg aagtttctcg tccctacgcg tagcggggcg
     5461 ctgcggataa tatcgctccc acagctcctc aacattaaga tgtgacaacg tcaccgtcgt
     5521 taaaatcacc aacgccacaa aagagaacaa gccgacacca gcctggatat gggcataatc
     5581 ctgtaccttt atagaagcaa cgccaatgcc gaccaggtag atatccagca ttacccactc
     5641 tttaagacgc tcaagcatca gcagtaccgg acgtaaattc attcccagtc ggttaccaaa
     5701 ccataaatat gctatggagg acaccagaat gaggggggca ccaataacgc agaaaaagac
     5761 catcgccccc gttatcgtat cgccctgttt ggtcatttgc cagatgcctt gcatgacgtt
     5821 ggcgtcgata cgaataccta acagccagat atgcaacagc ggttcgcccc aggcaaacgg
     5881 catcagcaac agcatggtga aagccattgc cgccaggcgc gttagcgacc agtcgcgccc
     5941 gtcgcgaatt tttgcctgac agcgcggaca ataggcactc tgatgagaat ttatctcggg
     6001 caggctaaac agcatgtcac attgcgggca acgttggtaa tcaccacgcg gcagttcctc
     6061 gccgattgcc ctcactgtta tcttttttgt cggcgtaatt tgtggtgtgt taagagccat
     6121 gacgtgccaa atgtaataag aattaacatt aatattaacc catgattgga atgatcttga
     6181 gcattaacgc atttaatgct tattttaata ggctggcagt tccaggtaag acaaccaagt
     6241 gattatataa tgaacaaaac agaattttac gcggatttaa atcgcgactt taacgcgctg
     6301 atggcgggag aaaccagttt tctggcaacg cttgcgaaca ccagtgcgtt gttatacgag
     6361 cgtctcactg acgtaaactg ggcaggtttt tatttgcttg aggacgatac actggtacta
     6421 ggaccatttc agggcaaaat tgcctgtgtc cggatacccg tcgggcgcgg ggtgtgcggc
     6481 actgcggttg cccgcaatca agtgcagcgt atcgaggatg ttcacgcgtt tgacgggcat
     6541 attgcctgtg atgcggcgag taattctgaa attgttctgc cgctggtggt gaaaaatcag
     6601 attattggtg ttcttgacat cgatagtacc gtcttcggtc gctttacaga cgaggacgag
     6661 caaggcttac gtcagcttgt ggcacagctt gaaaaagtgc ttgcaacgac ggattacaaa
     6721 aaattctttg cgagcgtcgc aggataatca acggataacg tagcatttac tggtagcgtc
     6781 attataatga cgcctgttca tgcctgcgct tgttggctac gtccgttgta atcaggaaat
     6841 ttcatggaaa atcaacctaa gttgaatagc agtaaagaag taatcgcgtt tctggccgaa
     6901 cgttttcccc actgtttcag tgcggaaggt gaagcgcgtc cgctgaaaat cggtattttt
     6961 caggatttgg tcgatcgtgt tgccggggaa atgaacctga gcaaaacgca attacgatcc
     7021 gctttacgtc tctacacttc gagctggcgt tatctttacg gtgttaaacc cggcgcaacg
     7081 cgtgtcgatc ttgacggcaa cccatgcggt gagctggacg agcaacatgt agagcatgct
     7141 cgcaagcagc ttgaagaagc gaaagcgcgt gttcaggcac agcgtgctga acagcaagcg
     7201 aaaaaacgcg aagctgccgc agctgctggt gagaaagaag acgcaccgcg ccgcgaacgc
     7261 aagccacgtc cgaccacgcc acgccgcaaa gaaggcgctg aacgtaaacc tcgtgcgcaa
     7321 aagccggtag agaaagcgcc aaaaacagta aaagcacctc gcgaagaaca gcacacccca
     7381 gtttctgaca tttcagctct gactgtcgga caagccctga aggtgaaagc gggtcaaaac
     7441 gcgatggatg ccaccgtatt agaaatcacc aaagacggcg tccgcgtcca gctgaattcg
     7501 ggtatgtctt tgattgtgcg cgcagaacac ctggtgttct gaaacggagg ccgggccagg
     7561 catgaacatg ttttttaggc ttaccgcgtt agctggcctg cttgcaatag caggccagac
     7621 cttcgctgta gaagatatca cgcgtgctga tcaaattccg gtattaaagg aagagacgca
     7681 gcatgcgacg gtaagtgagc gcgtaacgtc gcgcttcacc cgttctcatt atcgccagtt
     7741 cgacctcgat caggcatttt cggccaaaat ctttgaccgc tacctgaatc tgctcgatta
     7801 cagccacaac gtgctgctgg caagcgatgt tgaacagttc gcgaaaaaga aaaccgagtt
     7861 aggcgatgaa ctgcgttcag gcaaactcga cgttttctac gatctctaca atctggcgca
     7921 aaagcgccgt tttgagcgtt accagtacgc tttgtcggta ctggaaaagc cgatggattt
     7981 caccggcaac gacacttata accttgaccg cagcaaagcg ccctggccga aaaacgaggc
     8041 tgagttgaac gcgctgtggg acagtaaagt caaattcgac gagttaagcc tgaagctggc
     8101 aggaaaaacg gataaagaaa ttcgtgaaac cctgacacgc cgctacaaat ttgccattcg
     8161 tcgtctggcg caaaccaaca gcgaagatgt tttctcgctg gcaatgacgg cgtttgcgcg
     8221 tgaaatcgac ccgcatacca actatctttc cccgcgtaat accgaacagt tcaacactga
     8281 aatgagtttg tcgctggaag gtattggcgc agtgctgcaa atggatgatg actacaccgt
     8341 tatcaattcg atggtggcag gtggtccggc agcgaagagt aaagctatca gcgttggtga
     8401 caaaattgtc ggtgttggtc aaacaggcaa gccgatggtt gacgtgattg gctggcgtct
     8461 tgatgatgtg gttgccttaa ttaaagggcc gaagggcagt aaagttcgtc tggaaatttt
     8521 acctgctggt aaagggacca agacccgcac tgtaacattg acccgtgaac gtattcgtct
     8581 cgaagaccgc gcggttaaaa tgtcggtgaa gaccgtcggt aaagagaaag tcggcgtgct
     8641 ggatattccg ggcttctatg tgggtttgac agacgatgtc aaagttcaac tgcagaaact
     8701 ggaaaaacag aatgtcagca gtgtgatcat cgacctgcgt agcaatggcg gtggggcgtt
     8761 gaccgaagcg gtatcgctct caggtctgtt tattcctgcg ggtcccattg ttcaggtccg
     8821 cgataacaac ggcaaggttc gtgaagacag cgataccgac gggcaggtgt tctataaagg
     8881 cccgctggtg gtactggttg accgtttcag tgcttcggct tcagaaatct ttgccgcggc
     8941 aatgcaggat tacggtcgtg cgctggttgt gggtgaaccg acgtttggta aaggcaccgt
     9001 tcagcaatac cgttcattga accgtattta cgatcagatg ttacgtcctg aatggccagc
     9061 gctgggttct gtgcagtaca cgatccagaa attctatcgc gttaacggcg gcagtacgca
     9121 acgtaaaggc gtaacgccag acatcatcat gccgacgggt aatgaagaaa cggaaacggg
     9181 tgagaaattc gaagataacg cgctgccgtg ggatagcatt gatgccgcga cttatgtgaa
     9241 atcaggcgat ttaacggcct ttgggccgga gctgctgaag gaacataatg cgcgtatcgc
     9301 gaaagatcct gagttccaga acattatgaa ggatatcgcg cgcttcaacg ctatgaagga
     9361 caagcgcaat atcgtttctc tgaactacgc tgtgcgtgag aaagagaata atgaagatga
     9421 tgcgacgcgt ctggcgcgtt tgaacgaacg ctttaaacgc gaaggtaaac cggagttgaa
     9481 gaagctggat gatctaccga aagattacca ggagccggat ccttatctgg atgagacggt
     9541 gaatatcgca ctcgatctgg cgaagcttga aaaagccaga cccgcggaac aacccgatcc
     9601 cgtcaagtaa tatcaatcag gcacaagaaa ttgtgcctga ttttttaaca gcggcaagat
     9661 gccgtaaatc agatgctaca aaatgtaaag ttgtgtcttt ctggtgactt acgcactatc
     9721 cagacttgaa aatagtcgcg taacccatac gatgtgggta tcgcatattg cgttttgtta
     9781 aactgaggta aaaagaaaat tatgatgcga atcgcgctct tcctgctaac gaacctggcc
     9841 gtaatggtcg ttttcgggct ggtactgagc ctgacaggga tacagtcgag cagcgttcag
     9901 gggctgatga tcatggcctt gctgttcggt tttggtggtt ccttcgtttc gcttctgatg
     9961 tccaaatgga tggcattacg atctgttggc ggggaagtga tcgagcaacc gcgtaacgaa
    10021 agggaacgtt ggctggtcaa tactgtagca acccaggctc gtcaggcagg gatcgctatg
    10081 ccgcaagtgg ctatctacca tgcgccggac atcaacgctt ttgcaaccgg tgcgcgccgt
    10141 gatgcctctc tggttgctgt cagcaccggt ttgctgcaga acatgagccc ggatgaagcc
    10201 gaggcggtaa ttgctcacga aatcagtcac atcgccaatg gtgatatggt taccatgacg
    10261 ctgattcagg gcgtggtgaa caccttcgtt atctttattt cccgtattct ggcgcagctt
    10321 gccgcgggtt ttatgggcgg aaatcgtgat gaaggtgaag agagcaacgg caacccgctg
    10381 atctactttg cggttgcaac ggttctggaa ctggtgtttg gtattctggc gagcattatc
    10441 accatgtggt tctcgcgtca tcgtgaattc catgctgatg ccggttcggc aaaactggtt
    10501 ggtcgcgaga aaatgattgc cgcgctgcag cgcctgaaaa ccagctatga accgcaagaa
    10561 gcaaccagca tgatggcttt ctgcattaac ggtaagtcga aatcgctcag tgagttgttc
    10621 atgactcacc cgccgctgga taaacgaatt gaagctctgc gtacgggtga atacctgaag
    10681 taatatgaag taaatactaa aaggcgcgtc ctgatcgacg cgcttttttt atgccctgga
    10741 tcgtggctga gtgatacgta aaccactcac acaggcagca atcactgcca gaatagccgc
    10801 agccatcagc gaaacgtgcg taccattatc accgaactga tttagcatca gcgccaccag
    10861 cgccgcgcca ctactctgac ccagtagacg agccgttcct aacatgccac tggccccacc
    10921 gctacgttcg cgcggagcgg aggtaataat ggtgtggtta ttgggtgact ggaataagcc
    10981 aaatccagcg ccacataaga tcatcggcca gataatattg atatccgcag gtgacgcggg
    11041 cagcagaacc agggaaaaaa gccccgcagc catgatgaac aaccctaaag cacccagcaa
    11101 tcctgcatgt acgcgttcaa tcaaatagcc tgccagcgga gccatcacca tcgttgctaa
    11161 cggccacggt gtcagaagta aacctgtttc gacttcacta cgcccgagca cggtttgcag
    11221 gtaaaagggc agggaaacca ttgccagcat ttgtgcgcag aaagagcaaa cagatgtgca
    11281 aatagaaagt gaaaacagcg ggatacgcag taaatccacc ggtagcagcg gtacgggaag
    11341 agaaagctgg cggcgaataa agaaaatacc aacaacaacc attaccacca gttccgcagc
    11401 aattaacgtc agcgattgcc cctgagcgaa accactcaac gcagtgataa gcaggccgaa
    11461 ggttaacgcg ttcatcacgg cgctgggcag gtcgaaacgg ggtttactgg cgcgagaacc
    11521 attgggtggc agaaaacgca tcgccagaag cagggcgata atacctaacg gtacgttgat
    11581 taaaaataac catttccagg atgcgatgga gaggattgct gcagcaattg tcggcccggc
    11641 agcagaagaa acggcaacaa taaacgagtt tatgcccatc cctctcccca gaaaacgttg
    11701 tggatagatc aggcggataa gtgcggtatt aacgctcatc aacgctgcac cgccgaaacc
    11761 ttgtatgaca cgcgcaaggg tgagcatttg cagcgaatca gaaagggcgc agaacagtga
    11821 agacaacaga aaaacgacca gaccgcattt ataaatacgt cgatagccaa acatatcgcc
    11881 cagaaacgaa aacgagagca gggagatgac aatggcgatt tgataggcgt tcactaccca
    11941 gatggatctg gctggcgtgg catgaaggtc cgtggcgatt gttggcaggg cgacgttggc
    12001 gattgcgccg tcaaggacag ccatcgaaat gccaatcaca atggttaata tcgcaccgta
    12061 tcgctggggc aatggcaggc cgtcggcctg aacttttggc ataggaattt tatatctttg
    12121 gtgaataatc aacaggggta tgattctagc actgatattc acggtggatg tcgcagaatt
    12181 gtaactaacc ggcaaaagat ggattgcgac caccctggtg cgaaattata ataaaaaaca
    12241 gttctgattt ttataaaaca ctcgcaatga ggtgataaat ggctaacgca gatctggata
    12301 aacagcctga ttctgtatct tccgtgctaa aagtttttgg cattttgcag gcgctgggtg
    12361 aagagcgcga aatagggata accgagctgt cgcagcgcgt catgatgtca aaaagcaccg
    12421 tttatcgctt tttacagacc atgaaaacct taggttatgt ggcgcaggaa ggggagtcgg
    12481 agaaatattc cctgaccctg aaattgtttg aactgggcgc tcgcgcgtta caaaacgtcg
    12541 atttaattcg tagcgcagat atccagatgc gtgagatctc ccgcctgacc aaagaaacta
    12601 tccacctcgg cgcactggac gaagacagta ttgtttacat tcacaaaatt gactctatgt
    12661 acaatttgcg catgtattca cggattgggc gtcgtaatcc gctgtacagc accgcgattg
    12721 gtaaggtact gctggcatgg cgcgatctcg atgaagtgaa gcaaattctt gagggcgtgg
    12781 agtataaacg cagtaccgag cggaccatca ccagtacaga agcgttatta cccgttctgg
    12841 accaggtgcg cgagcagggg tatggcgaag ataacgaaga gcaggaagaa gggctgcgat
    12901 gcattgcggt accggtattt gatcgctttg gcgtggtcat tgccggtttg agcatctcct
    12961 tcccgacgtt gcgtttctct gaagagcgtt tacaggaata tgtcgcaatg ttgcataccg
    13021 cagcgcgcaa aatttctgcc caaatgggtt atcacgacta tccgttctga tgagagtaag
    13081 aacctgtcgg aatatcaaac agacaggttc tttatttagc atgagaaaaa taaagttgaa
    13141 ggtgcgttat attaa
//
LOCUS       DAZKHF020000120        13139 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2201S31241
            SAMN43267800-rid25106413.denovo.121, whole genome shotgun sequence.
ACCESSION   DAZKHF020000120 DAZKHF020000000
VERSION     DAZKHF020000120.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267800
            Sequence Read Archive: SRR30307263
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 13139)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13139)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 13139)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350581.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350581.2
            Genome Coverage       :: 151x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:21:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,652
            CDSs (total)                      :: 5,557
            Genes (coding)                    :: 5,320
            CDSs (with protein)               :: 5,320
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 237
            CDSs (without protein)            :: 237
            Pseudo Genes (ambiguous residues) :: 0 of 237
            Pseudo Genes (frameshifted)       :: 102 of 237
            Pseudo Genes (incomplete)         :: 130 of 237
            Pseudo Genes (internal stop)      :: 52 of 237
            Pseudo Genes (multiple problems)  :: 41 of 237
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13139
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267800-rid25106413.denovo.121"
                     /serotype="O 26:H11"
                     /isolate="2201S31241"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-01"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            49..1107
                     /locus_tag="ACHOSC_RS13995"
                     /old_locus_tag="pgaptmp_002799"
     CDS             49..1107
                     /locus_tag="ACHOSC_RS13995"
                     /old_locus_tag="pgaptmp_002799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-grasp domain-containing protein"
                     /protein_id="HGU4548707.1"
                     /translation="MNKKIWFMEGLSSQRDIIQGVKSFAQKNNFAITVFASHRNERNE
                     ILSVADYSLTEPEDPQKRLQFIQETIQTYGIHHIHTGRNSQWFEEHRSAIEPTGATLT
                     TGATGVDWLTLADEKVTFAQFMEQKGLPVVPSWRVNTLAELKTHLAAPPFTDSPVCVK
                     PVTGIYGMGFWRFDDSASPMAVFNHPEHRLVSPQQYIAAASAAESFKPLVLMPYLPGP
                     EFSVDILADKGEILAAVGRRKEGAIQYLVNEGSAWELACDCARVMKADGLVNVQTRND
                     VNGNPVLLETNMRPSGGVGYTLHSGVNLPGLFAAFKLGLMSEDMVRQSAKNTFSPVAV
                     RSITDVIAYPESLSNLLN"
     gene            1117..2259
                     /locus_tag="ACHOSC_RS14000"
                     /old_locus_tag="pgaptmp_002800"
     CDS             1117..2259
                     /locus_tag="ACHOSC_RS14000"
                     /old_locus_tag="pgaptmp_002800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosyltransferase domain-containing
                     protein"
                     /protein_id="HGU4548708.1"
                     /translation="MSDILEDMIYRRTLSCGTIQVTRDQGEVSLDDLFDIAERRNPKR
                     AFLFVSKVLGRHIPVPPSVMRQAYRQLASQFPSTLTGPVLFIGMAETAVGLGAGVFDE
                     VRHQHRESVYLTSTRHPVDGTLLCEFKEEHSHATDHLIYLPDDEEKRRRVTNARTLVL
                     IDDEATTGNTFINLLSALRNTGKLQHIEQVIAVTLTDWSGNALSERTPLPVTSVSLVS
                     GQWGWTPLPDAPVPDMPKVNVTSRGEWDIQGKQSWGRLGMLAPAADLGHEVSVHKGER
                     ILVLGTGEFVWEPFLLAERLEAAGAQAFYGSTTRSPVAVGYAIESAISFTDNYGLGIP
                     NFVYNVAHQQFDRILVCTETPAESIDTQLLKALAEVAPVVEIVTYE"
     gene            2252..3025
                     /locus_tag="ACHOSC_RS14005"
                     /old_locus_tag="pgaptmp_002801"
     CDS             2252..3025
                     /locus_tag="ACHOSC_RS14005"
                     /old_locus_tag="pgaptmp_002801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4548709.1"
                     /translation="MNKPVVLSDLDDTLFQTRRKMVDELALEPFRTGAVDRTLNPRSF
                     MTEEQSMLVDWLLEQAELIPVTARGTEEISRVRIPFHSWAITTHGAVILTPEGKPDEE
                     WKAHMLGQLAPYQEKLTSMQRLITEMMDAKGINAWARLNFEYGETAVYMVMKHRDSTR
                     LDELNAIADEIETVFPTEGFYIHRNSNNVAWLPTPVEKGLAVSWLLEKLRAERGVFPV
                     IGLGDSLSDHRFMKLCSWFGIPRQSQFADAISQRIFGEN"
     gene            3027..4106
                     /locus_tag="ACHOSC_RS14010"
                     /old_locus_tag="pgaptmp_002802"
     CDS             3027..4106
                     /locus_tag="ACHOSC_RS14010"
                     /old_locus_tag="pgaptmp_002802"
                     /EC_number="3.4.22.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309373.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine protease StiP family protein"
                     /protein_id="HGU4548710.1"
                     /translation="MQPHETFTGSYQPGDVEFLLKPVVIEMTPVEKKEELIQSGKKHY
                     SDMLSQEPAPTQWHLDLFHRALDRGAERLAKEVTQLAIALAERFGDEPIVLASLVRAG
                     VPLGVMLHQALRDMGKTSWHYGISIIRDRGIDGAALDVIEERHGTSGIVFVDGWTGKG
                     AITGELVRALKDRPGYPEQPRLVVLADPCGCSWLAASDDDWLIPFGIMGAPVSGLISR
                     SVWSSEGLHGCMVCEHLSEFECSRMLADTVAHFRKKLTPSSLAPLSWNTESARVLWQT
                     SRDVIAFLADEFKVDSVNRIKPGIAEATRAVLRRVPDHVFVRSIDDPDVALLVGLARE
                     KGIVVTEMGGTLGQYRAVTIIKKVL"
     gene            4106..5062
                     /locus_tag="ACHOSC_RS14015"
                     /old_locus_tag="pgaptmp_002803"
     CDS             4106..5062
                     /locus_tag="ACHOSC_RS14015"
                     /old_locus_tag="pgaptmp_002803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HpcH/HpaI aldolase/citrate lyase family protein"
                     /protein_id="HGU4548711.1"
                     /translation="MKNRLSPWNLGATLYMPATREDIADAVLHGKIPGLRSLVICLED
                     AVSEADIPVALKNLEHLLHELSNSMSSLGKNDWPLVFIRPRHAEMGRWLKAHYDLSAV
                     DGFVLPKFTLSSLAEWWDIMVGTHLCMMPTLETEDVFDVVQMRELATRLEEHPCHDRI
                     IALRIGGNDLMNVVSLRRPRDLTLYDSPMGYVIKMLVSVFGPRDFALTAPVCEHIDDH
                     AVMARELALDMAHGLVGKTAIHPGQIEVIQNALMVTQGEHSDALRILNSTQAVFKSQG
                     AMCEPATHRRWAAGILDRARFYGLQNEQSADGIRLLTVTQHH"
     gene            5073..6281
                     /locus_tag="ACHOSC_RS14020"
                     /old_locus_tag="pgaptmp_002804"
     CDS             5073..6281
                     /locus_tag="ACHOSC_RS14020"
                     /old_locus_tag="pgaptmp_002804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4236 domain-containing protein"
                     /protein_id="HGU4548712.1"
                     /translation="MGFRFRKSINIIPGVRLNLSNGAPSLSVGPRGASVSFGSRGTYA
                     NLGLPGTGLSYRTRLDRAARSRGENRTATDPGLRQALEQKAAELMSAVTAIRNIHELT
                     PDPKTGISWAELEAVYLHNRTSPFQVPAPVRPEKPDYLVLPEKPAESEGISFLGKWFE
                     SESAKAERHAENLRRWQQELIDVERENTLRQHRYQQQRTAWAEQYANWKFEAEEHEKR
                     LATAQADARQQFRTDAAFFESYLAGVLAETEWPRETLVAFEVKPELSAVLLDVDLAEI
                     EDFPDKIYGVNARGTELTEKAMTQKAVRENYAHHVHGCLFRLVGIVLHTLPFDNVIVS
                     GFTQRVSKRTGYLEDEYILSCKCTRSQMSSVNFAGIKHIDPVEALGDQPVIRKMSSTF
                     IFQPIEPLTL"
     gene            6299..6766
                     /gene="terW"
                     /locus_tag="ACHOSC_RS14025"
                     /old_locus_tag="pgaptmp_002805"
     CDS             6299..6766
                     /gene="terW"
                     /locus_tag="ACHOSC_RS14025"
                     /old_locus_tag="pgaptmp_002805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tellurium resistance protein TerW"
                     /protein_id="HGU4548713.1"
                     /translation="MQLNTRQARIFKLANLLGTGKPVSAADIITSLECSEPTLTRALK
                     ELRESYSAEIKYSKAGHSYHLVNPGQLDKKTLRRMNEALAQNAELKTGESTGKVVLDK
                     DKKTAVSLSLRMRILRKIDRLAALSDSTRSEAVEKLALHSVDELIKEYSAKKS"
     gene            7027..7356
                     /locus_tag="ACHOSC_RS14030"
                     /old_locus_tag="pgaptmp_002806"
     CDS             7027..7356
                     /locus_tag="ACHOSC_RS14030"
                     /old_locus_tag="pgaptmp_002806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family insertion sequence element accessory
                     protein TnpB"
                     /protein_id="HGU4548714.1"
                     /translation="MSKPRWTLDQKKHHVAAWRASGLTREQYCELYDIPFKSLRQWPQ
                     DVAKAEKRARAPEIIPVSVSGSSGMTDGRPLSDEPVTLFLPGGIRMCCQPSQLTDVFR
                     ALRHADA"
     gene            7343..7723
                     /gene="tnpB"
                     /locus_tag="ACHOSC_RS14035"
                     /old_locus_tag="pgaptmp_002807"
     CDS             7343..7723
                     /gene="tnpB"
                     /locus_tag="ACHOSC_RS14035"
                     /old_locus_tag="pgaptmp_002807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000950651.1"
                     /note="TnpB, as the term is used for proteins encoded by
                     IS66 family insertion elements, is considered an accessory
                     protein, since TnpC, encoded by a neighboring gene, is a
                     DDE family transposase; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family insertion sequence element accessory
                     protein TnpB"
                     /protein_id="HGU4548715.1"
                     /translation="MLTPDNVFLVVKPVDMRRGIDTLTQYVQNELNAAWHDGAAFVFT
                     NKVRSRIKVLRWDKHGVWLCTRRLHRGSFRWPRKGDATWHLTQDEFHWLVFGVEWQQV
                     KGHDLAKWVYQEEPEDAQSNAITQ"
     gene            7766..8596
                     /locus_tag="ACHOSC_RS14040"
                     /old_locus_tag="pgaptmp_002808"
                     /pseudo
     CDS             7766..8596
                     /locus_tag="ACHOSC_RS14040"
                     /old_locus_tag="pgaptmp_002808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001546148.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family transposase"
     gene            complement(8627..10240)
                     /locus_tag="ACHOSC_RS14045"
                     /old_locus_tag="pgaptmp_002809"
     CDS             complement(8627..10240)
                     /locus_tag="ACHOSC_RS14045"
                     /old_locus_tag="pgaptmp_002809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000080195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS66-like element IS682 family transposase"
                     /protein_id="HGU4548716.1"
                     /translation="MSRKYLIRITELERLLSEQAEALRQKDQQLSLVEETEAFLRSAL
                     ARAEEKIEEEEREIEHLRAQIEKLRRMLFGTRSEKLQREVEQAEAQLKQREQESDRYS
                     GREDDPQVPRQLRQSRHRRPLPAHLPREIHRLEPEESCCPECGSELDYLGEVSAEQLE
                     LVSSALKVIRTVRVKKACTKCDCIVEAPAPSRPIERGIAGSGLLARVLTGKYCEHLPL
                     YRQSEIFARQGIELSRALLSNWVDACCQLMTLLNDTLYRYVMNTRKVHTDDTPVKVLA
                     PGRKKAKTGRIWTYVRDDRNAGSSEPPAVWFAYSPDRQGKHPVQHLRPFRGILQADAF
                     SGYDRLFSAEREGGALTEVACWAHARRKIHDVYISSKSATAEEALKRISELYAIEDEI
                     RGLPESERLAVRQQRSKVLLTSLHEWMVEKNGTLSKKSRLGEACSYVLNQWDALCYYS
                     DDGLAEADNNAAERALRAVCLGKKNFMFFGSDHGGERGALLYGLIGTCRLNGIDPEAY
                     LRHILSVLPEWPSNRVDELLPWNVVLTNK"
     gene            complement(10271..10621)
                     /gene="tnpB"
                     /locus_tag="ACHOSC_RS14050"
                     /old_locus_tag="pgaptmp_002810"
     CDS             complement(10271..10621)
                     /gene="tnpB"
                     /locus_tag="ACHOSC_RS14050"
                     /old_locus_tag="pgaptmp_002810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309365.1"
                     /note="TnpB, as the term is used for proteins encoded by
                     IS66 family insertion elements, is considered an accessory
                     protein, since TnpC, encoded by a neighboring gene, is a
                     DDE family transposase; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family insertion sequence element accessory
                     protein TnpB"
                     /protein_id="HGU4548717.1"
                     /translation="MISLPSGTRIWLVAGVTDMRKSFNGLGEQVQHVLDENPFSGHLF
                     IFRGRRGDTIKILWADADGLCLFTKRLEEGQFIWPAVRDGKVSITRSQLAMLLDKLDW
                     RQPKTSRLNALTML"
     gene            complement(10618..11043)
                     /locus_tag="ACHOSC_RS14055"
                     /old_locus_tag="pgaptmp_002811"
     CDS             complement(10618..11043)
                     /locus_tag="ACHOSC_RS14055"
                     /old_locus_tag="pgaptmp_002811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001373091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HGU4548718.1"
                     /translation="MEQKILSSEPRRSFSNEFKLQMVKLASQPGASVARIAREHDIND
                     NLLFKWLRLWQNERRISRRLPVTTSSGAGVELLPVEITPDEQKEPMAALTPLLSTPSQ
                     STVSASSCKVEFRHGNMTLENPSPELLTVLIRELTGRGR"
     gene            11097..11848
                     /locus_tag="ACHOSC_RS14060"
                     /old_locus_tag="pgaptmp_002812"
                     /pseudo
     CDS             11097..11848
                     /locus_tag="ACHOSC_RS14060"
                     /old_locus_tag="pgaptmp_002812"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001546148.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family transposase"
BASE COUNT         3138 a         3463 c         3449 g         3089 t
ORIGIN      
        1 tcacttctca atcataaatt tgcatggcgt tccggataat taaaaaaaat gaacaagaaa
       61 atttggttta tggaaggttt atcctcccag cgagatatta ttcagggggt aaaatcattt
      121 gcacaaaaaa ataattttgc cattaccgtt tttgcctccc accgtaacga aagaaatgaa
      181 atcctttccg ttgccgatta ttctttgact gaacctgaag atcctcaaaa acgtcttcag
      241 tttatccagg aaaccattca gacctacggc atccaccata ttcatactgg ccgtaacagc
      301 cagtggtttg aagaacaccg ttcagccatt gaaccgaccg gtgccaccct tactaccggt
      361 gcaacgggcg tcgactggtt aactctggct gacgaaaaag ttacttttgc tcagtttatg
      421 gagcaaaagg gtctcccggt cgtaccatcc tggcgggtga atacgctggc agaattaaag
      481 acacacctcg cggccccgcc gttcactgac agcccggtat gcgtgaagcc ggtgacgggt
      541 atctatggca tgggattctg gcgctttgat gacagtgctt cgcctatggc cgtctttaat
      601 catcccgaac atcgtctggt cagtccgcaa cagtatattg cagcagcatc agctgctgag
      661 tcgtttaaac cccttgtttt gatgccgtac ctgccaggcc cggaattttc cgtcgatatc
      721 ctcgcggata agggcgaaat actcgcagcc gtgggacgcc gtaaggaagg ggctatccag
      781 tatctggtaa acgaaggaag cgcctgggaa ctggcgtgtg actgcgcccg tgttatgaag
      841 gccgacgggc tggtgaatgt tcagacgcga aacgatgtga atggcaaccc ggtgctgctt
      901 gaaaccaaca tgcgtccgtc agggggggtg ggttataccc ttcacagcgg tgtgaacctt
      961 cctgggttat ttgctgcctt taagctcggt ctgatgtctg aagatatggt acgccagagc
     1021 gctaaaaaca ccttttctcc ggttgcggtg agatccatta cggatgtaat tgcatacccg
     1081 gaatcactct ctaaccttct gaattaaggc gtatttatgt ctgatatcct cgaagatatg
     1141 atttatcgcc gtaccctctc atgcggtacg attcaggtaa cccgtgacca gggtgaagtt
     1201 tcgctcgatg acctgtttga catcgctgaa agacgtaacc cgaaacgcgc ctttctgttt
     1261 gtcagcaaag tactcggcag gcacattccc gtgccaccct cagtcatgcg acaggcctac
     1321 agacagcttg ccagccagtt cccctcgaca ctgacaggac ccgtactgtt tatcggtatg
     1381 gctgaaaccg ccgttgggct tggggccggt gtatttgatg aagtgcgcca ccagcatcgt
     1441 gaatctgtct atctgacctc tacccgtcac ccggttgatg gcacgttgct ttgcgagttc
     1501 aaggaagaac acagccacgc caccgatcat ctgatctatc tgccagatga tgaagagaaa
     1561 agacgtcgtg ttaccaacgc acgaacgctg gttttgattg atgacgaggc aaccaccggt
     1621 aataccttta ttaacctgct ttctgccctg cgtaataccg gcaagcttca acatattgaa
     1681 caggttatcg ccgtaacgct gaccgactgg agtggcaatg ccctgtcgga gcgcaccccc
     1741 ttgcccgtca cttcggtttc gcttgtaagc ggtcagtggg gatggacccc attacctgat
     1801 gcacctgtcc cggacatgcc gaaagtcaac gtaacttcac gaggagaatg ggacatccag
     1861 gggaaacagt catggggccg actggggatg ctcgcacctg cggccgatct cggccatgag
     1921 gtctccgtcc acaaggggga acgtattctg gttctcggga ccggggaatt cgtctgggag
     1981 ccgttcctgc ttgctgaacg gctcgaagct gccggagcac aggcatttta tggatcgacc
     2041 acccgctccc ctgtcgccgt tggttatgcc attgagtccg ccatttcctt tacggataac
     2101 tacgggctgg gcatccccaa ttttgtctat aacgtcgccc accagcagtt tgaccgcatt
     2161 cttgtgtgta ctgagacacc cgcagaaagt attgacacgc agcttcttaa agcgctggct
     2221 gaggttgcgc ccgtcgtgga gattgttacc tatgaataaa cccgttgttc tgagcgacct
     2281 tgacgacacg ctctttcaga cccgtcgtaa aatggtggac gagctggccc tggagccatt
     2341 ccgtaccggt gccgttgatc gcaccctgaa tccacgaagc tttatgacgg aagaacagtc
     2401 catgttggtg gactggctcc tggagcaggc tgaactcata cccgttaccg cacggggaac
     2461 tgaagaaatc agccgcgtac ggatcccttt ccactcctgg gcaatcacaa cgcacggggc
     2521 cgtcattctc acgcctgaag gcaaacccga cgaggagtgg aaggcccata tgctcggcca
     2581 gctggctccc taccaggaaa agctgacatc gatgcagcgt ctgatcactg aaatgatgga
     2641 cgcaaaagga atcaatgcct gggcaaggct gaacttcgaa tacggtgaaa cggcggttta
     2701 tatggtgatg aaacaccgcg acagcacccg ccttgacgag ctcaatgcca ttgcagatga
     2761 gatagaaacg gtgtttccga ccgagggctt ctacatccac cgcaacagta ataacgtggc
     2821 ctggcttccc accccggttg agaaagggct ggcagtcagc tggctgcttg aaaaacttcg
     2881 ggctgaacga ggagttttcc ccgtaattgg tttgggcgac agcctgagcg atcatcgttt
     2941 tatgaaactg tgcagctggt ttggcatccc gcgtcagagt cagtttgcag atgccatttc
     3001 acagcgaatt tttggagaaa attaaaatgc agccacatga aacatttacc ggctcatacc
     3061 agcccggtga cgtggaattt ctgctaaagc cggttgtcat tgagatgacg ccggttgaga
     3121 aaaaagaaga actgattcag tccgggaaga aacattattc ggacatgctc agccaggagc
     3181 cagcgcctac acaatggcat cttgatttgt ttcaccgggc gctggatcgg ggcgcagaga
     3241 ggcttgcaaa agaagtcaca cagctcgcta ttgctctggc cgaacgcttc ggtgatgagc
     3301 ccattgtact ggccagtctt gtcagggctg gcgttccgct cggcgttatg ctgcaccagg
     3361 ccctgcgtga catggggaaa acctcatggc attacggtat cagcattatc cgggatcgtg
     3421 gaattgacgg tgcagccctc gacgtcattg aagagcggca tggtaccagc ggtattgtct
     3481 ttgttgacgg atggacaggt aaaggcgcaa ttaccggaga gcttgtacgc gcactgaaag
     3541 atcgcccggg ctatcctgag cagccgcgac tggttgtcct ggccgatccc tgtggctgct
     3601 cctggcttgc agccagtgat gatgactggc tcattccttt tggcatcatg ggggcgccgg
     3661 tatcaggcct gatctcccgg tcagtatggt cctctgaagg attacacggg tgcatggttt
     3721 gcgagcatct cagtgaattc gaatgcagcc ggatgcttgc cgataccgtc gctcatttcc
     3781 gtaagaagtt aacaccgtca tccctcgctc ccctgagctg gaatacggaa tcagcccggg
     3841 tcttatggca gacaagtcgc gacgttatcg cgttcctggc cgatgaattt aaagtggaca
     3901 gcgtcaatcg tattaaaccc ggtattgctg aagcaacccg ggctgtatta cgtcgggtac
     3961 cggaccatgt atttgtgcgt tctattgacg acccggacgt tgccttgctt gtagggcttg
     4021 ctcgtgaaaa gggaatagtt gttacagaaa tggggggaac cctcggccag tatcgggctg
     4081 taaccattat caagaaggta ctctgatgaa aaatcgtctt tctccctgga atctgggagc
     4141 cacgctatac atgcctgcaa cacgggaaga tattgctgat gccgtcctgc acgggaaaat
     4201 cccgggcttg cgctctctgg tgatttgcct ggaggatgct gtcagtgaag cagacatccc
     4261 cgtcgccctc aaaaatctgg agcacctgct gcacgagctc agtaacagca tgagtagcct
     4321 gggtaaaaat gactggcctc tggtgttcat tcgcccccgg catgccgaaa tgggcagatg
     4381 gttaaaagcg cattatgatc tttccgctgt cgacgggttt gtgctgccta aatttaccct
     4441 cagttcgtta gctgaatggt gggatatcat ggtcgggact cacctgtgca tgatgccaac
     4501 gctggaaaca gaagatgtct ttgacgtggt tcagatgcgc gagctggcta cccgtctgga
     4561 ggaacatccc tgccacgacc gcattattgc gcttcgaatc ggcggcaacg atcttatgaa
     4621 cgttgtgtcg cttcgccgtc cccgggacct gacgctgtat gacagcccga tgggctacgt
     4681 catcaaaatg ctcgtttcgg tcttcggccc gcgtgatttt gccctgaccg cgcctgtatg
     4741 tgagcatatt gacgatcatg ccgttatggc cagagagctg gctcttgata tggcacacgg
     4801 gcttgttggg aaaacggcca ttcacccggg tcagatagag gtcattcaaa acgcgctgat
     4861 ggtcactcag ggtgagcatt ctgacgctct gcggatcctg aactctaccc aggccgtgtt
     4921 taagtcgcag ggagcaatgt gtgaacccgc cacacatcgc cgctgggcgg ctggcattct
     4981 ggacagggct cgtttttatg ggttacagaa cgagcaaagc gctgatggaa tcagattact
     5041 taccgtgacc cagcatcatt aaggaaatca taatggggtt caggttcaga aagtcgatca
     5101 acattattcc tggcgttcgc ctcaacctga gtaacggtgc accgagcctg agtgtcgggc
     5161 cgagaggtgc ttccgtttct tttggtagcc gggggaccta tgccaatctg ggcttgcccg
     5221 gtaccgggct gagttaccgt acccggcttg accgggccgc gcgttccaga ggtgaaaacc
     5281 ggacggcaac cgacccgggg ctcagacagg cgcttgagca gaaagccgct gaactcatgt
     5341 cagcggtaac cgcaatccgt aatattcacg agctgacgcc ggatccaaaa acaggcatca
     5401 gctgggcaga gctggaagca gtatacctgc ataacagaac gtcgcctttt caggttccgg
     5461 caccggtgcg tccagaaaag ccagactacc ttgtattgcc ggaaaagcct gccgagagcg
     5521 aaggcattag ttttttgggt aaatggtttg aatcggaatc agctaaagct gagcgccacg
     5581 ccgaaaatct tcgccggtgg cagcaggagc tgattgatgt ggagcgtgag aatacccttc
     5641 gacagcaccg gtaccagcaa caacggacgg cctgggccga acagtatgca aactggaagt
     5701 ttgaagctga agaacatgaa aaacggctcg ccacggctca ggcagatgcc cggcagcagt
     5761 tccggacaga cgccgcgttt ttcgaatcat acctggcggg tgtgctggca gaaactgaat
     5821 ggccgcgtga aacgcttgtt gcatttgaag taaagccgga gctatcagca gtcctgctgg
     5881 acgttgattt agctgagatt gaagatttcc ctgataagat ttacggcgtt aatgcccggg
     5941 gaacggagct gacggaaaaa gccatgacgc aaaaagccgt acgcgaaaac tatgcccacc
     6001 acgtccatgg ctgcttgttc cgcctggtcg gtatcgtttt acatacgcta cctttcgaca
     6061 acgtgattgt gtcaggcttt acgcaacggg tcagtaagcg gaccggctat ctggaggatg
     6121 agtatatcct gtcctgcaaa tgcactcgca gccagatgtc gtcagtaaat tttgcaggca
     6181 taaaacacat tgatccggtt gaagcgttag gcgatcaacc ggttattcga aagatgagca
     6241 gtaccttcat ttttcagcct attgaaccac taacccttta agcaggctaa atcttacgat
     6301 gcaattaaat accagacagg cccggatttt taagcttgcc aacttactgg gcaccggcaa
     6361 gcccgtttcc gctgccgaca ttataaccag tctggaatgc tctgagccga cattaacccg
     6421 ggccctcaaa gagctacgcg agtcatattc tgcggagatc aaatacagca aggcaggtca
     6481 ttcctatcat ctcgttaatc cgggccagtt ggataaaaag accctccgcc ggatgaatga
     6541 agcgctcgca cagaatgctg aactgaaaac cggtgagtcc acagggaagg tcgtactcga
     6601 taaggataaa aaaacagccg tgtcattatc gctacgcatg cggattctaa gaaaaattga
     6661 ccgtctggct gcgctgagtg actcgacccg aagtgaagcg gtagagaagc tggctctgca
     6721 ctccgttgat gaactgataa aagaatacag cgctaaaaag tcctgacgct aaatctcaga
     6781 ccggggtgaa aactccggtt ttgtcatatt tcacctctgt attggcgtga tgcaaaatgg
     6841 catctgaatc gatcatggtt tggcatccat ttccgatcac agtttggcat cacaatcgat
     6901 cacgatttgg catgcttccg ctcattgatt ggcatcctgg tgtcgttacg gggaagtcat
     6961 cttcacaggg atcttcagtc agttagcatt accgacatcc attcaccaac ttaccggaga
     7021 taatcaatgt caaaaccccg atggacgctt gatcagaaaa aacatcacgt tgccgcatgg
     7081 cgcgccagcg gccttacccg tgaacagtac tgtgaacttt acgacattcc gtttaaatcc
     7141 ctgcgccagt ggccgcagga tgtcgcaaaa gccgaaaaac gagccagagc accagaaatt
     7201 attcctgttt cagtatctgg ctcttccgga atgactgatg gcagaccatt gtcggatgag
     7261 cctgtcactc tcttcctgcc cggtggtatc cggatgtgct gccagccttc tcagttaacc
     7321 gatgttttcc gggctcttcg ccatgctgac gcctgataac gtattcctgg tcgtaaaacc
     7381 cgtcgatatg cgccggggta tcgacacact gacacagtac gtccagaacg aacttaacgc
     7441 tgcatggcat gacggtgctg cttttgtctt caccaataaa gtccgctccc gcataaaagt
     7501 tctccggtgg gacaaacatg gcgtatggct gtgtacccga cgactgcacc ggggaagctt
     7561 ccgctggccc cggaaaggtg atgccacatg gcatctcaca caggatgagt ttcactggct
     7621 ggtctttggg gttgagtggc aacaggtgaa agggcatgac ctggcaaaat gggtatacca
     7681 ggaagaaccc gaagatgctc agagcaacgc aataactcaa tgaaatataa tgaatatcca
     7741 tgcgtgatgt catataatcc accgcatgga tatctcgctt ctcagtacca ccagtgaccc
     7801 tgaacagctt cgcgctctgg caattgccat ggtgcaaaaa gtcatggctg agaatgcaga
     7861 acttcagaac cgtatccgca tcctggaaga gcagatgaag ctcgcacgcc agcagcgttt
     7921 cggtaaaaaa tgcgaatcgc tggccgggat gcagcgttcc ctgttcgaag aggatgttga
     7981 cgcggatatc gcagaaatct cggcgcatct ggacaaactg cttcctcaga ccggggacga
     8041 agaaaaaaca acaacacgtc ctgttcgtaa gcccttgcct tcacctcttc cccgtgctga
     8101 aaaggttatc ccgccagctg aggaacgttg tcctgactgt gatgcccccc tgcacttcat
     8161 tcgcgatgaa gtcagcgaga agctggaata tattccggca caggtcgtgg tcaaccgcta
     8221 tatccgcccg cagtacagct gtccctgctg tgagaaagtg ttcagcggaa aaatgccggc
     8281 acatatcctt ccgaaaagtg ctgttgagcc atcggtcatc gcacaggttg tcatcagcaa
     8341 atataccgac catctgcccc tgtatcgcca gcagcacatt ttctcccgaa tgggggtgga
     8401 gctccccgtc agtacaatgg cggatatggt cggtgttgct ggcgctgcac tggccccgct
     8461 ggcaaaattg ttacgccacg agcttctgac acgggatgtt attcatgcag atgaaacgtc
     8521 cctgaggctc cttgataccc ggaaaggcgg taaatcctgc tccggctggc tgtgggctta
     8581 cgtcagcgga gaaagagtaa gcgtcaacgg agcaccgtat tgacgcttat ttattggtga
     8641 gtactacgtt ccatggcagg agttcatcaa ctcggttgga aggccattct ggcagtacgc
     8701 tcaggatatg gcgcagatac gcttccggat cgataccgtt cagacggcag gtgccgatca
     8761 gcccgtacaa cagtgctcca cgctcgccgc cgtgatcgct gccaaagaac ataaagtttt
     8821 tctttccgag acagactgca cgaagcgctc tttccgcagc attattatcc gcctccgcca
     8881 gaccgtcatc actgtaataa cagagggcat cccactgatt cagtacatag ctgcacgctt
     8941 cgcccagtct ggattttttc gacagcgtac cattcttctc caccatccat tcatgcagtg
     9001 acgtcagtaa cactttgctt cgctgctgcc tgacggcaag acgctctgac tccggtaatc
     9061 cccgtatttc atcctcgatg gcgtacagtt cactgattcg cttcagggct tcttctgccg
     9121 tcgcactttt gctgctgatg tatacatcgt ggatttttcg ccgggcatgg gcccagcacg
     9181 caacttctgt cagtgcacca ccttcacgtt ctgcactgaa cagccgatcg taaccgctga
     9241 acgcatccgc ctgcaggata ccccggaagg gacgaaggtg ttgtaccgga tgttttccct
     9301 gcctgtctgg tgagtaggcg aaccagaccg ccggtggctc tgatgagccc gcattccggt
     9361 catcccggac atacgtccag atgcgtcctg tttttgcctt ttttctgccc ggtgccagca
     9421 cttttactgg tgtgtcgtca gtgtgaacct tgcgggtgtt catcacgtaa cggtacaggg
     9481 tatcattcag cagagtcatt aactgacaac atgcgtcaac ccagttggag agtaatgccc
     9541 ggctcagttc gatgccctgg cgggcaaaga tttcactctg acgatacagt ggcaggtgtt
     9601 cgcagtattt tcccgttaac acgcgggcaa gtaatccgga gcccgcgatg ccgcgctcta
     9661 tcgggcggga cggcgctggc gcttcaacta tacagtcaca ttttgtacag gcttttttta
     9721 cccgaacagt gcggatcact ttcagggcgc tactcaccag ttccagctgc tcagcactga
     9781 cttcccccag ataatccagc tcactgccac actccgggca acaactttct tcaggctcca
     9841 gacggtgtat ttcacgggga agatgtgccg gtaacggacg acgatgacgt gactgtcgca
     9901 actggcgggg aacctgcggg tcatcctcac gcccactgta acgatcgctt tcctgttcgc
     9961 gttgtttcag ttgggcctca gcctgttcaa cctcacgctg cagtttttca gaacgggtac
    10021 cgaacaacat ccggcgcagt ttttctatct gggccctcag atgttctatt tcccgctcct
    10081 cctcttcgat cttttcctcg gcacgtgcca gtgcagagcg caggaaggcc tccgtctctt
    10141 caaccagact cagttgctga tctttctgac ggagggcttc agcctgctca gagagcagcc
    10201 tttccagctc agtgatacga atgaggtatt tccgactcat gaccgttttt ataatccggc
    10261 catgacattt ttacaacatt gtcagtgcat taaggcggga tgttttgggc tgacgccagt
    10321 ccagtttatc gaggagcatt gccagctgcg agcgggtaat ggatacctta ccgtcacgca
    10381 ccgcaggcca gataaactgg ccttcctcca gacgtttggt gaacaggcac agaccatcag
    10441 catcagccca cagaatttta atcgtgtcac cccgtcggcc gcgaaagata aacaggtgac
    10501 cggagaaggg gttctcatcc agcacatgtt gtacctgttc tcccagaccg ttgaaggatt
    10561 tacgcatatc agtaacgccg gcaaccagcc agattcgagt gcctgatggg agcgagatca
    10621 tcgtcccctc ccggtcagtt cacggatcaa caccgtgagc agctctggtg aaggattttc
    10681 cagcgtcatg ttaccgtggc ggaactcaac tttacaggaa ctggcactga ctgtgctttg
    10741 tgaaggagtg gataaaagcg gagtaagagc cgccataggc tctttctgct catcaggcgt
    10801 tatctcaaca ggtaataatt caacgccagc gccagaagag gttgttaccg gaagacgccg
    10861 cgatatacgc ctttcgttct gccagagcct gagccatttg aacaggaggt tatcattgat
    10921 atcgtgttcc cgggcaatac gggcaacaga ggctcctggt tgtgaagcca gtttaaccat
    10981 ttgaagttta aactcatttg aaaatgatct gcggggttct gaggataata ttttctgttc
    11041 cataacaggt gtccactagt tgaaaaggtg ggcacctacg ttaccaatac tggcttaatg
    11101 gctacatacg gcggtcagtt tacgcttacg agaaagaagc ggtccgccgg tcgtctgttt
    11161 tgactcgcag accggacggg ccctcagata tcctgaggcc tggctgcagg gctggtgtgg
    11221 cggtacgctg gtgagtgacg gatacagcgt gtacaaatct ctggcggata atgaccccgg
    11281 tattacatct gcctgttgct ggagccacgc aaggcgcggt ttcgcaaatc tgtataaggc
    11341 cagcagggag ccccgtgcag ccatggcgct gagaaaaatc gcgggtcttt atcgcattga
    11401 gaagttcatc cgtgaacgtc ctgtggaaaa aatacggcaa tggcgacaac ggtattcccg
    11461 gccgatagtg aatgacctgt ttgcctggcc tgaagagcag gagccgtgct gtccgcccga
    11521 cggaccacta aacaaagcga ttaattatat cctgaaccgg cgcgatgaac tgagctgctt
    11581 cctgagtgac ggagccgtgc ccctggacaa caatatctgc gagcgtgcta tccgcccggt
    11641 ggtcatgggg cggaaagcat ggttattcgc cggttcactg atggccggga accgcgcggc
    11701 acagataatg agccttctgg aaacggcaaa acgtaatggt ctggagccgc atgcctggct
    11761 gacagacgtt ctgacgcgtc taccggagtg gccggaagac cgactggaag agttgctgcc
    11821 ccttgaggga tttaccttct ccgggtgagt gatacctgcc atcaggtgtt cgtgcagcgg
    11881 gccataaccc gcagtcggga gttgaacgcc tgacgacagg aaatgagcca gagcagcgac
    11941 cacgcgggct gacagtgagt cagaacctga atcagcccgg agccattccc ggggctgtac
    12001 ttcatcacct gccatcagta cccgtgtcag tacttcgcga cctgacgcaa ttacccgact
    12061 cagttcttca gaacccgcct ccgataccag cctggcggtc gccccggcgc aacagaataa
    12121 atcaaccatc gtcagactgc gatcattcgt atgcatcgct gatgatgcgc ccgaaaaacg
    12181 cgtcaatatg agttcgccgg aatattactg acgacacggt ttgtagttac gttccttcgg
    12241 tgagtgagta cctgtgcgtc agggtaacgc aatcagttca ggtttagcgg ggtgtcaagt
    12301 cggtttggct aaccgaccgc agaattaact gtacctttaa aggtcgcccc cgatatatct
    12361 gcctgtcaga catatcgccg attcggtatc tcactacata aaactcctta aaaaacaatg
    12421 cgattctgtt ggtacgaagc tttcagtaag tcagtccgga gaagcgccag tacgatacag
    12481 aatgaggttg aaaaaaacgt tcacgtctgg tcacttcttc aaatgttaaa aaacatctcg
    12541 taaagagcca gtttatgcgg gttcgttgag tagacgtgcg agcgtcactt tgtcagcttt
    12601 aacgctgacg aaagtgacgc ccgcacagct tcaaactcca catgcacacg caagcgacca
    12661 aatttcaaat ggtcttatgc cgtgcgcaca gtcataagaa gcgcatgcgg aaagtcagaa
    12721 aatatcttaa gacgcagatt ttaaaggcgt ttttatgcca gtctgccggg aatacacagt
    12781 agtccggttg tccggaagca ccacggtaat ccgaaggaaa aatcaacgta cctcaatcac
    12841 cgtagtgata tccgttccca gaggctaaac ggatgtacca ccataaaggc atcctctttc
    12901 aggctacgag gatattgcaa tcacccgaag gcgtttctct cagattgctg aaacattggc
    12961 tggcagtcac ccgaaggcgt ccctctcaga ctgctggaac attggctggt cgcaaaagcg
    13021 acagttaatg caaatacagg ttaacaaaaa gggaaatctg ccgtcaaccc aaccatatga
    13081 acatgttgat ttcattatga atgtgatgtt ctaatataaa ttaatcagta acttcataa
//
LOCUS       DAZKHF020000121        13079 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2201S31241
            SAMN43267800-rid25106413.denovo.122, whole genome shotgun sequence.
ACCESSION   DAZKHF020000121 DAZKHF020000000
VERSION     DAZKHF020000121.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267800
            Sequence Read Archive: SRR30307263
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 13079)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13079)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 13079)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350581.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350581.2
            Genome Coverage       :: 151x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:21:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,652
            CDSs (total)                      :: 5,557
            Genes (coding)                    :: 5,320
            CDSs (with protein)               :: 5,320
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 237
            CDSs (without protein)            :: 237
            Pseudo Genes (ambiguous residues) :: 0 of 237
            Pseudo Genes (frameshifted)       :: 102 of 237
            Pseudo Genes (incomplete)         :: 130 of 237
            Pseudo Genes (internal stop)      :: 52 of 237
            Pseudo Genes (multiple problems)  :: 41 of 237
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13079
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267800-rid25106413.denovo.122"
                     /serotype="O 26:H11"
                     /isolate="2201S31241"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-01"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            complement(178..1833)
                     /gene="treC"
                     /locus_tag="ACHOSC_RS14065"
                     /old_locus_tag="pgaptmp_002813"
     CDS             complement(178..1833)
                     /gene="treC"
                     /locus_tag="ACHOSC_RS14065"
                     /old_locus_tag="pgaptmp_002813"
                     /EC_number="3.2.1.93"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709962.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008788 - alpha,alpha-phosphotrehalase
                     activity [Evidence IEA];
                     GO_process: GO:0005991 - trehalose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha,alpha-phosphotrehalase"
                     /protein_id="HGU4548719.1"
                     /translation="MTNLPHWWQNGVIYQIYPKSFQDTTGSGTGDLRGVIQRLDYLHK
                     LGVDAIWLTPFYISPQVDNGYDVANYTAIDPIYGTLDDFDELVTQAKSRGIRIILDMV
                     FNHTSTQHAWFREALNKESPYRQFYIWRDGEPETPPNNWRSKFGGSAWRWHAESEQYY
                     LHLFAPEQADLNWENPAVRAELKKVCEFWADRGVDGLRLDVVNLISKDPRFPEDLDGD
                     GRRFYTDGPRAHEFLHEMNRDVFTPRGLMTVGEMSSTSLEHCQRYAALTGSELSMTFN
                     FHHLKVDYPGGEKWTLAKPDFVALKTLFRHWQQGMHNVAWNALFWCNHDQPRIVSRFG
                     DEGEYRVPAAKMLAMVLHGMQGTPYIYQGEEIGMTNPHFTRITDYRDVESLNMFAELR
                     NDGRDADELLAILASKSRDNSRTPMQWSNGDNAGFTAGEPWIGLGDNYQQINVEAALA
                     DDSSVFYTYQKLIALRKQEAILTWGNYQDLLPNSPVLWCYRREWKGQTLLVIANLSRE
                     IQPWQPGQMRGNWQLVMHNYEEASPQPCAMNLRPFEAVWWLQK"
     gene            complement(1883..3304)
                     /gene="treB"
                     /locus_tag="ACHOSC_RS14070"
                     /old_locus_tag="pgaptmp_002814"
     CDS             complement(1883..3304)
                     /gene="treB"
                     /locus_tag="ACHOSC_RS14070"
                     /old_locus_tag="pgaptmp_002814"
                     /EC_number="2.7.1.201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_function: GO:0015574 - trehalose transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0015771 - trehalose transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS trehalose transporter subunit IIBC"
                     /protein_id="HGU4548720.1"
                     /translation="MMSKINQTDIDRLIELVGGRGNIATVSHCITRLRFVLNQPANAR
                     PKEIEQLPMVKGCFTNAGQFQVVIGTNVGDYYQALIASTGQAQVDKEQVKKAARQNMK
                     WHEQLISHFAEIFFPLLPALISGGLILGFRNVIGDLPMSNGQTLAQMYPSLQTIYDFL
                     WLIGEAIFFYLPVGICWSAVKKMGGTPILGIVLGVTLVSPQLMNAYLLGQQLPEVWDF
                     GMFSIAKVGYQAQVIPALLAGLALGVIETRLKRIVPDYLYLVVVPVCSLILAVFLAHA
                     LIGPFGRMIGDGVAFAVRHLMTGSFAPIGAALFGFLYAPLVITGVHQTTLAIDLQMIQ
                     SMGGTPVWPLIALSNIAQGSAVIGIIISSRKHNEREISVPAAISAWLGVTEPAMYGIN
                     LKYRFPMLCAMIGSGLAGLLCGLNGVMANGIGVGGLPGILSIQPSYWQVFALAMVIAI
                     IIPIVLTSFIYQRKYRLGTLDIV"
     gene            complement(3423..4373)
                     /gene="treR"
                     /locus_tag="ACHOSC_RS14075"
                     /old_locus_tag="pgaptmp_002815"
                     /pseudo
     CDS             complement(3423..4373)
                     /gene="treR"
                     /locus_tag="ACHOSC_RS14075"
                     /old_locus_tag="pgaptmp_002815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418662.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0005991 - trehalose metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="trehalose operon repressor TreR"
     gene            4555..4608
                     /gene="mgtL"
                     /locus_tag="ACHOSC_RS14080"
                     /old_locus_tag="pgaptmp_002816"
     CDS             4555..4608
                     /gene="mgtL"
                     /locus_tag="ACHOSC_RS14080"
                     /old_locus_tag="pgaptmp_002816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_003933616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mgtA regulatory leader peptide MgtL"
                     /protein_id="HGU4548721.1"
                     /translation="MEPDPTPLPRRRLKLFR"
     gene            4749..7445
                     /gene="mgtA"
                     /locus_tag="ACHOSC_RS14085"
                     /old_locus_tag="pgaptmp_002817"
     CDS             4749..7445
                     /gene="mgtA"
                     /locus_tag="ACHOSC_RS14085"
                     /old_locus_tag="pgaptmp_002817"
                     /EC_number="7.2.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015444 - P-type magnesium transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015693 - magnesium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium-translocating P-type ATPase"
                     /protein_id="HGU4548722.1"
                     /translation="MFKEIFTRLIRHLPSRLVHRDPLPGAQQTVNTVVPPSLSAHCLK
                     MAVMPEEELWKTFDTHPEGLNQAEVESAREQHGENKLPAQQPSPWWVHLWVCYRNPFN
                     ILLTILGAISYATEDLFAAGVIALMVAISTLLNFIQEARSTKAADALKAMVSNTATVL
                     RVINDKGENGWLEIPIDQLVPGDIIKLAAGDMIPADLRILQARDLFVAQASLTGESLP
                     VEKAATTRQPEHSNPLECDTLCFMGTTVVSGTAQAMVIATGANTWFGQLAGRVSEQES
                     EPNAFQQGISRVSMLLIRFMLVMAPVVLLINGYTKGDWWEAALFALSVAVGLTPEMLP
                     MIVTSTLARGAVKLSKQKVIVKHLDAIQNFGAMDILCTDKTGTLTQDKIVLENHTDIS
                     GKTSERVLHSAWLNSHYQTGLKNLLDTAVLEGTDEESARSLASRWQKIDEIPFDFERR
                     RMSVVVAENTEHHQLVCKGALQEILNVCSQVRHNGEIVPLDDIMLRKIKRVTDTLNRQ
                     GLRVVAVATKYLPAREGDYQRADESDLILEGYIAFLDPPKETTAPALKALKASGITVK
                     ILTGDSELVAAKVCHEVGLDAGEVVIGSDIETLSDDELANLAQRTTLFARLTPMHKER
                     IVTLLKREGHVVGFMGDGINDAPALRAADIGISVDGAVDIAREAADIILLEKSLMVLE
                     EGVIEGRRTFANMLKYIKMTASSNFGNVFSVLVASAFLPFLPMLPLHLLIQNLLYDVS
                     QVAIPFDNVDDEQIQKPQRWNPADLGRFMIFFGPISSIFDILTFCLMWWVFHANTPET
                     QTLFQSGWFVVGLLSQTLIVHMIRTRRVPFIQSCASWPLMIMTVIVMIVGIALPFSPL
                     ASYLQLQALPLSYFPWLVAILAGYMTLTQLVKGFYSRRYGWQ"
     gene            complement(7652..8038)
                     /gene="ridA"
                     /locus_tag="ACHOSC_RS14090"
                     /old_locus_tag="pgaptmp_002818"
     CDS             complement(7652..8038)
                     /gene="ridA"
                     /locus_tag="ACHOSC_RS14090"
                     /old_locus_tag="pgaptmp_002818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418664.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-iminobutanoate/2-iminopropanoate deaminase"
                     /protein_id="HGU4548723.1"
                     /translation="MSKTIATENAPAAIGPYVQGVDLGNMIITSGQIPVNPKTGEVPA
                     DVAAQARQSLDNVKAIVEAAGLKVGDIVKTTVFVKDLNDFATVNATYEAFFTEHNATF
                     PARSCVEVARLPKDVKIEIEAIAVRR"
     gene            complement(8111..8572)
                     /gene="pyrI"
                     /locus_tag="ACHOSC_RS14095"
                     /old_locus_tag="pgaptmp_002819"
     CDS             complement(8111..8572)
                     /gene="pyrI"
                     /locus_tag="ACHOSC_RS14095"
                     /old_locus_tag="pgaptmp_002819"
                     /EC_number="2.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000148566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009347 - aspartate carbamoyltransferase
                     complex [Evidence IEA];
                     GO_process: GO:0006207 - 'de novo' pyrimidine nucleobase
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate carbamoyltransferase regulatory
                     subunit"
                     /protein_id="HGU4548724.1"
                     /translation="MTHDNKLQVEAIKRGTVIDHIPAQIGFKLLSLFKLTETDQRITI
                     GLNLPSGEMGRKDLIKIENTFLSEDQVDQLALYAPQATVNRIDNYEVVGKSRPSLPER
                     IDNVLVCPNSNCISHAEPVSSSFAVRKRANDIALKCKYCEKEFSHNVVLAN"
     gene            complement(8585..9520)
                     /gene="pyrB"
                     /locus_tag="ACHOSC_RS14100"
                     /old_locus_tag="pgaptmp_002820"
     CDS             complement(8585..9520)
                     /gene="pyrB"
                     /locus_tag="ACHOSC_RS14100"
                     /old_locus_tag="pgaptmp_002820"
                     /EC_number="2.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004070 - aspartate carbamoyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006207 - 'de novo' pyrimidine nucleobase
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate carbamoyltransferase"
                     /protein_id="HGU4548725.1"
                     /translation="MANPLYQKHIISINDLSRDDLNLVLATAAKLKANPQPELLKHKV
                     IASCFFEASTRTRLSFETSMHRLGASVVGFSDSANTSLGKKGETLADTISVISTYVDA
                     IVMRHPQEGAARLATEFSGNVPVLNAGDGSNQHPTQTLLDLFTIQETQGRLDNLHVAM
                     VGDLKYGRTVHSLTQALAKFDGNRFYFIAPDALAMPQYILDMLDEKGIAWSLHSSIEE
                     VMAEVDILYMTRVQKERLDPSEYANVKAQFVLRASDLHNAKANMKVLHPLPRVDEIAT
                     DVDKTPHAWYFQQAGNGIFARQALLALVLNRDLVL"
     gene            complement(9524..9658)
                     /gene="pyrL"
                     /locus_tag="ACHOSC_RS14105"
                     /old_locus_tag="pgaptmp_002821"
     CDS             complement(9524..9658)
                     /gene="pyrL"
                     /locus_tag="ACHOSC_RS14105"
                     /old_locus_tag="pgaptmp_002821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyr operon leader peptide"
                     /protein_id="HGU4548726.1"
                     /translation="MVQCVRHFVLPRLKKDAGLPFFFPLITHSQPLNRGAFFCPGVRR
                     "
     gene            complement(9939..10334)
                     /gene="yjgH"
                     /locus_tag="ACHOSC_RS14110"
                     /old_locus_tag="pgaptmp_002822"
     CDS             complement(9939..10334)
                     /gene="yjgH"
                     /locus_tag="ACHOSC_RS14110"
                     /old_locus_tag="pgaptmp_002822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HGU4548727.1"
                     /translation="MVERTAVFPAGRHSLYAEHRYSAAIRSGDLLFVSGQVGSREDGT
                     PEPDFQQQVRLAFDNLHATLAAAGCTFDDIIDVTSFHTDPEKQFEDIMTVKNEIFSAP
                     PYPNWTAVGVTWLAGFDFEIKVIARIPEL"
     gene            complement(10465..11178)
                     /gene="bdcA"
                     /locus_tag="ACHOSC_RS14115"
                     /old_locus_tag="pgaptmp_002823"
     CDS             complement(10465..11178)
                     /gene="bdcA"
                     /locus_tag="ACHOSC_RS14115"
                     /old_locus_tag="pgaptmp_002823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HGU4548728.1"
                     /translation="MGAFTGKTVLILGGSRGIGAAIVRRFVTDGANVRFTYAGSKDAA
                     EHLAQETGATAVFTDSADRDAVIDVVRKSGALDILVVNAGIGVFGDALELNADDIDRL
                     FKINIHAPYHASVEAARQMPEGGRILIIGSVNGDRMPVAGMAAYAASKSALQGMARGL
                     ARDFGPRGITINVVQPGPIDTDANPANGPMRDMLHSLMAIKRHGQPEEVAGMVAWLAG
                     PEASFVTGAMHTIDGAFGA"
     gene            11249..11842
                     /gene="bdcR"
                     /locus_tag="ACHOSC_RS14120"
                     /old_locus_tag="pgaptmp_002824"
     CDS             11249..11842
                     /gene="bdcR"
                     /locus_tag="ACHOSC_RS14120"
                     /old_locus_tag="pgaptmp_002824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709951.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HGU4548729.1"
                     /translation="MVTKKQSRVPGRPRRFAPEQAVSAAKVLFHQKGFDAVSVAEVTD
                     YLGINPPSLYAAFGSKVGLFSRVLNEYVGTEAIPLADILRDDRPVGECLVEVLKEAAR
                     RYSQNGGCAGCMVLEGIHSHDPQARDIAVQYYHAAETTIYDYIARRHPQSAQCVTDFM
                     STVMSGLSAKAREGHSIEQLCATAALAGEAIKTILKE"
     gene            11987..12439
                     /gene="tabA"
                     /locus_tag="ACHOSC_RS14125"
                     /old_locus_tag="pgaptmp_002825"
     CDS             11987..12439
                     /gene="tabA"
                     /locus_tag="ACHOSC_RS14125"
                     /old_locus_tag="pgaptmp_002825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313256.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxin-antitoxin biofilm protein TabA"
                     /protein_id="HGU4548730.1"
                     /translation="MIIGNIHNLQPWLPQELRQAIEHIKAHVTAETPKGKHDIEGNRL
                     FYLISEDMTEPYEARRAEYHARYLDIQIVLKGQEGMTFSTQPAGTPDTDWLADKDIAF
                     LPEGVDEKTVILNEGDFVVFYPGEVHKPLCAVGAPAQVRKAVVKMLMA"
     gene            12562..>13079
                     /gene="yjgL"
                     /locus_tag="ACHOSC_RS14130"
                     /old_locus_tag="pgaptmp_002826"
     CDS             12562..>13079
                     /gene="yjgL"
                     /locus_tag="ACHOSC_RS14130"
                     /old_locus_tag="pgaptmp_002826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YjgL"
                     /protein_id="HGU4548731.1"
                     /translation="MAKISDLNCYQHMASADNVKPKNEVLNTWCVGMNDFARNAEGQD
                     SRRNILSPGAFLEFLAKIFTLGYVDFSKRSNEAGRNMMAHIESSSYIKNNDGSEIMNF
                     VMNNPEGERADSSKVIIEISLSTTTTMGTRQGHTAIIFPQSDFLSFRYEGKSFERKDD
                     SSLHLITNKVLA"
BASE COUNT         3186 a         3208 c         3411 g         3274 t
ORIGIN      
        1 aaggatgttt taactttttt tgatgtagct caaagtaaaa agcagagcgt acggatgacg
       61 ggcgctacag cgttatgtaa attttttaat gaatttgctg gttgaaaaat caacaaaaac
      121 aacatgctga cagacaaaac cccggaatga ccggcattac cggggcttag ggaagattta
      181 cttctgtaac caccagacag cctcaaaagg ccgtaaattc atggcacagg gttgtggtga
      241 ggcttcttcg tagttatgca tcacaagctg ccagttgccg cgcatttgcc ctggctgcca
      301 gggttggatc tcacggctaa ggttggcaat gaccagcaag gtttgcccct tccattcacg
      361 gcgatagcac cacaatacag ggctgtttgg cagcagatcc tggtaattgc cccatgtcag
      421 gatggcttct tgcttacgca gtgcgattaa cttttggtag gtgtaaaaca ccgaggagtc
      481 atcggccagc gcggcttcta cgttgatttg ttgatagtta tcgcccaggc caatccacgg
      541 ttcgccagcc gtaaacccgg cattatcgcc gttgctccat tgcatgggcg tgcggctgtt
      601 gtcacgggat ttactggcga ggattgccaa taactcgtcg gcatcacgac catcgttgcg
      661 cagctcggca aacatattga ggctctccac gtcgcgatag tcagtaatgc gcgtgaaatg
      721 cgggttggtc atgccaatct cttcgccttg gtagatatac ggcgttccct gcatgccatg
      781 cagcaccatc gccagcattt ttgccgcagg cacgcggtat tcaccttcat caccaaagcg
      841 agaaacaatg cgcggctgat cgtggttaca ccagaacaag gcattccatg ccacgttgtg
      901 cattccttgt tgccagtggc ggaacaatgt tttcaacgcc acaaagtcag gtttagccag
      961 cgtccatttt tcaccaccgg gataatcgac cttcaggtga tgaaaattaa aggtcatcga
     1021 caattcactg cctgtcagtg ccgcgtatcg ctggcaatgc tcaaggctgg tggaggacat
     1081 ttcacctacg gtcattaacc cgcgtggcgt aaacacatcg cggttcatct cgtgcaaaaa
     1141 ctcgtgtgct cgtggcccgt cggtgtagaa gcgacgcccg tcgccatcca ggtcttcagg
     1201 gaaacgcggg tctttggaga tcagattcac cacatccagg cgcaacccgt cgaccccacg
     1261 atcggcccag aattcacaga cttttttcag ctctgcgcgt accgctggat tctcccagtt
     1321 gagatccgcc tgttctggtg caaagagatg caaatagtac tgttcgcttt ccgcatgcca
     1381 gcgccacgca ctaccgccaa attttgaacg ccagttgttc ggtggcgttt ctggttcgcc
     1441 atcgcgccag atataaaact ggcggtaagg gctttctttg ttcagcgcct cgcgaaacca
     1501 ggcatgttgg gtagaggtat ggttaaacac catatcgaga atgatacgaa tcccgcgcga
     1561 ttttgcctgc gtcaccagtt cgtcaaaatc gtccagcgtg ccgtagatgg gatcaatcgc
     1621 cgtatagttc gctacgtcgt aaccgttatc gacctgggga gagatataaa agggggttag
     1681 ccagatggca tcaacgccca gtttatgcag atagtccagg cgttggataa cgccacgtaa
     1741 atcgccggtg ccgctacccg tggtgtcctg aaaactcttt ggataaatct ggtagataac
     1801 gccgttttgc caccagtggg gaagattagt cattacgtta ttcctgcaaa tgcgaggggg
     1861 cgcaattgcg ccccgaagaa aattaaacaa tatccagcgt gcccaggcgg tatttccgct
     1921 gatagataaa cgaggtgagt acaatcggga tgatgatggc gataaccatt gccagcgcaa
     1981 atacctgcca gtagctcggt tgaatcgaga gaattcccgg caggccgcct acgccgatac
     2041 cattcgccat aacgccgttc aggccgcata gtaatcctgc cagaccagaa ccaatcatcg
     2101 cgcacagcat cgggaagcga tatttcaggt tgatgccgta cattgcaggc tcagtgaccc
     2161 caagccaggc ggagatagcg gcaggcacgg agatctcgcg ttcattgtgc ttgcggctgg
     2221 aaatgataat gcctatcacg gcggagccct gagcgatatt cgacagcgca atcagcggcc
     2281 acactggcgt gccacccatg ctttgaatca tctgcaaatc aatagcaagg gtggtctggt
     2341 gtacaccggt gatcaccagc ggggcgtaca ggaagccaaa caatgctgcg ccaatcggag
     2401 caaagctgcc ggtcatcagg tgacgtaccg caaaggcaac gccatcgcca atcatgcgac
     2461 caaacggacc aatcagcgca tgggcgagga acaccgcgag gatcagcgaa cagacgggca
     2521 ccaccaccag atagaggtaa tccggcacga tgcgtttaag gcgagtttca ataacgccca
     2581 gcgccagccc ggctaacagt gccgggatca cctgcgcctg atagcctact ttggcgatgc
     2641 tgaacatgcc aaagtcccac acttccggca gctgctgccc gagcagataa gcgttcatca
     2701 gctggggaga aaccagtgtc acgccaagca cgataccaag gatcggcgtg ccgcccattt
     2761 ttttcaccgc tgaccagcaa ataccgaccg gcaggtagaa gaagatcgct tcaccgatca
     2821 accacagaaa atcgtagatc gtttgcaggg aagggtacat ttgcgccagc gtctgaccgt
     2881 tgctcatggg caaatcgccg atcacattgc gaaaaccgag gatcaaaccg ccgctaatca
     2941 acgcgggcag cagcgggaag aagatctccg cgaaatgaga gatcaactgc tcatgccatt
     3001 tcatattctg ccgggcggct ttttttacct gctctttatc aacctgcgcc tgtccggttg
     3061 acgcaatcag tgcttgatag taatcaccca cattggtgcc aatcaccacc tgaaattgcc
     3121 cggcattggt gaaacagcct ttcaccatgg ggagttgctc aatttctttc ggtctggcat
     3181 tggccggttg gttgaggaca aagcgcaggc gagtaataca gtggctcacc gtcgcaatat
     3241 tgccgcgccc gccgaccagt tcaatcaacc gatcgatatc cgtttggttt attttgctca
     3301 tcataaagcc ccatggcaga tgacattttt ggttggctgc agaatatatt gcgcggaaaa
     3361 atttaaaaac gggaacgttc ccgaaacgca gcgaagatca caatttatcg ttcaggaaac
     3421 gatcaggaca gggtggcggg gatgatgatt tgttgcggtt cgctgcgccc ggttacctgt
     3481 gcgatcaatt ggcaggccgc ctggcgtcca gcttcggcgt aaccaggatc tacggtgacg
     3541 atctccggat gaaggaattt cattaacggc gtattaccga cgctcgccag ttgcaaggtg
     3601 tcgatgcgtt gctcttgcag gtatttactt gcgccaagtg ccagcgtgtc ggttgcgcac
     3661 agtaaggcgg tagtttcagg cgtaatcact tttgctacgt tctcatagcc ttgcttcata
     3721 gcaagccctg gcagagcggc aacgggatgc agtttatgcg ctttgcagaa cgccaggtag
     3781 gcttcgtgac gtcgcttacc ggtcgtcacg tcactgtgcg gcacgccgag ataactgata
     3841 ttacgatgcc cctggtcata gagccgttgc atcaggatct tgattgcccc ttcgtcgtca
     3901 taacagaccg aagcaaagcc ttttgcgtca cgcgccagca gaaccagcga tgactgccag
     3961 tgggctaaca tttcttctgt tatgccggta aaaccgaaca gcactacgcc gtcgatatta
     4021 cgccgtttta ggatccccaa atgttcggca actaattgcg gggaaaactg actttccatc
     4081 atgattgggt cgtaaccttg ttcatagaac gctggcagca tggtttgaac ggcgagattt
     4141 tctgacaacg aatccaggcg ggtaacaatg atggcgacca ctttatcgct ttgcccacgc
     4201 atagcgcgcg cagagcggga aggggaaaat ccatgctgat tcatcactgc ttccacgcgc
     4261 tcgcgcgtgc gctggctcac gccgctttcg ttattcagca cccgggaaac cgtagatttc
     4321 cccacgccgc ttaagcgcgc gatgtctttg atggtcagcc gattttgcat cctgttgtcc
     4381 cgtaacgtgt tgtttaatta tttgaggcta actttacccg tgcattcagc aatgggtaaa
     4441 gtctggttta tcgttggttt agttgccagc aggtattata tcgccataaa tgctacgaat
     4501 attattggat tctccttatt atttgcggcg cttttttcac ttaccggagg ttatatggaa
     4561 cctgatccca cgcctctccc tcgacggaga ttaaaacttt ttcggtaagc ccgtcttttc
     4621 acggcgttac cggatgcgta aggccgtgac gttttaacgt ccctgctcag ctttattacc
     4681 ttcaggtaag gcttcgccac gcctgaagac atttctgtac tgtttcagac agtgcggagg
     4741 gactccttat gtttaaagaa atttttaccc ggctcattcg ccatttacct tcccgtctgg
     4801 ttcatcgtga tccattgcct ggcgcgcagc agacagtgaa tacggtggtc ccgccgtcct
     4861 taagtgcgca ttgcctgaaa atggcggtga tgcccgaaga agaattgtgg aaaacgttcg
     4921 acacccatcc ggaagggtta aatcaggcgg aagtggaatc tgcccgcgaa caacatggtg
     4981 aaaataaatt acccgcacaa caaccgtcgc cgtggtgggt acatttatgg gtctgctatc
     5041 gcaacccctt taatatttta ctcaccattc tcggcgctat ttcttacgcc acggaagatt
     5101 tatttgccgc gggcgttatc gcgctaatgg tcgccatttc tacgttgctg aactttattc
     5161 aggaagcacg ttccactaaa gcggcagatg ccctgaaagc gatggtcagc aatactgcga
     5221 cggtgctgcg cgtaattaac gacaaaggcg aaaatggctg gctggagatc ccgatcgacc
     5281 agctggtgcc cggcgatatt ataaaactgg cggcggggga tatgatcccg gcagatttac
     5341 gtatcttgca ggcgcgggat ctgttcgtcg ctcaggcgtc gttaaccggt gagtctctgc
     5401 ccgtagaaaa agcagctacc actcgccagc cggagcacag caatccgctg gagtgcgaca
     5461 ccctgtgttt tatgggcacc accgtggtga gcggcacggc acaagcaatg gtgattgcta
     5521 caggtgccaa tacctggttt ggtcaactgg cggggcgtgt tagtgagcag gaaagcgagc
     5581 cgaatgcctt tcagcaaggg atcagccgcg tcagtatgct gctgattcgc tttatgctgg
     5641 tgatggctcc ggtggtgctg ttaatcaatg gttacaccaa aggcgactgg tgggaagcgg
     5701 cactgtttgc gctttcggtc gcggtaggct taacgccgga aatgttgccg atgattgtta
     5761 cctcgacgct ggcgcgcggc gcagtaaagc tgtcgaaaca gaaagtgatc gtcaaacatc
     5821 tggatgctat tcagaacttt ggcgcaatgg atattctgtg cactgataaa actggcaccc
     5881 tgacacagga caaaattgtg ctggagaatc ataccgatat ctccggtaaa accagcgaac
     5941 gcgtgctgca tagcgcgtgg ttgaacagtc attatcagac cggacttaaa aacctgctcg
     6001 atacagcggt gctcgaaggt acggatgaag agtcagcgcg ctcgctggcc agtcgttggc
     6061 aaaaaatcga tgagattccg tttgatttcg agcgtcgccg gatgtcggtg gtagtggcag
     6121 aaaataccga gcaccatcag ctggtttgca aaggtgcatt gcaggaaatc ctcaatgtgt
     6181 gttcgcaggt gcgtcacaat ggcgagattg tgccgctcga tgacatcatg ctgcgtaaga
     6241 ttaagcgggt tactgatacg ctgaatcgtc aggggctgcg cgtggttgcg gtggcgacga
     6301 aatacctgcc agcgcgtgaa ggagattacc agcgggcgga tgaatccgac ctgatcctcg
     6361 aaggatatat tgctttcctt gatccgccaa aagagacaac tgctccggca ctgaaggcat
     6421 taaaagcgag tggaattacc gtaaaaatcc ttactggcga cagtgagtta gttgcagcga
     6481 aagtgtgcca tgaagtgggg ctggacgcgg gagaggtggt cattggtagt gatattgaaa
     6541 cgctatctga cgacgaactg gcaaatctcg cacagcgcac cacgctgttt gctcgtctga
     6601 cgccgatgca taaagaacgc attgtgacct tactgaagcg cgaagggcat gtggttggct
     6661 ttatgggcga tggtattaat gatgcgcccg ccttacgcgc tgcggatatc ggcatttctg
     6721 tggacggcgc ggtagatatt gcccgtgaag cggctgatat catcctgctg gaaaaaagcc
     6781 tgatggtgct ggaagagggg gttattgagg gacgtcgcac tttcgccaac atgctgaaat
     6841 acatcaaaat gacggcgagc tctaacttcg gtaatgtgtt cagcgtgctg gtggcgagtg
     6901 ctttcttgcc cttcctgccg atgttgccgt tacacttgct tattcagaac ctgctgtacg
     6961 atgtgtcaca ggtggcgatc ccgtttgata acgtcgacga cgagcaaatt caaaagccgc
     7021 agcgttggaa tccggcggat ctggggcgct ttatgatctt cttcggaccg atcagctcga
     7081 tcttcgatat tctgacgttt tgcctgatgt ggtgggtatt ccatgccaac acgccggaaa
     7141 cgcaaacgct gttccagtcg ggatggtttg tggtgggctt actgtcgcaa acgctgattg
     7201 tgcatatgat ccgtacccgc cgtgtgccgt ttattcagag ctgtgcatcg tggccgttaa
     7261 tgatcatgac cgtgatcgtg atgattgtcg ggatcgcatt gccgttttca ccgctggcca
     7321 gttatctgca gctgcaggcg ctgccgttaa gctatttccc gtggctggtt gcgattctgg
     7381 cagggtatat gacgttaacc cagttggtga aagggttcta tagccgtcgt tacggctggc
     7441 agtaaagaat aaactgggca cgatagcccc gattcagatt gcagacaaag tgagcgttgt
     7501 tcatgccgga tgcggcgtaa atgccttatc cggcctacaa attcaatcaa ttgcaggaat
     7561 cacgtagata ctgataagcg tagcgcatca ggcaattttg cgtttgtcat cagatttaat
     7621 ccgggcatga tagcccggat ttccatcaag attagcgacg aacagcgatc gcttcgatct
     7681 caatttttac gtctttcggc agacgggcaa cttcaacgca agaacgtgcc gggaaggtgg
     7741 cattgtgttc ggtgaagaag gcttcgtagg tagcattaac ggttgcgaag tcgttcagat
     7801 ctttcacaaa cacggtggtt ttaacgatgt cgcccacttt caggcctgcg gcttcgacga
     7861 tcgcttttac gttatccagc gactgacgtg cctgtgcagc gacgtctgcc ggtacttcgc
     7921 ccgttttcgg atttaccggg atctggccgg aggtgatgat catattgccc agatcaacgc
     7981 cctgtacgta aggaccgata gctgccggtg cattttccgt cgcgatagtt ttgctcatga
     8041 tttctccttt attacagcgg taaaaagtct ggctcattat agggagccag acttttatta
     8101 ccaaccgcaa ttaattggcc agcaccacat tatgggaaaa ctctttttca cagtatttgc
     8161 atttgagcgc gatgtcattg gcgcgttttc gcacggcaaa gctggaagaa accggttcgg
     8221 catggctgat acagttgctg ttcgggcaga ccagcacatt gtcgatgcgc tccggcagac
     8281 tggggcgcga tttacccacc acttcatagt tgtcgatacg gttaaccgtg gcttgcggcg
     8341 catacaatgc cagttgatct acctgatctt cactcaaaaa ggtattttcg attttgatca
     8401 gatctttgcg gcccatctcg ccagaaggca ggttcagacc aatggtgatg cgctgatccg
     8461 tttcggtcag cttgaacaga ctcaacagct taaaaccgat ctgggcgggg atatggtcaa
     8521 ttaccgtgcc gcgtttaata gcttcaacct gcaatttatt atcgtgtgtc atctctattt
     8581 ccccttacag taccagatcg cgattcagaa ccagtgccag taacgcctgg cgagcgaaaa
     8641 tcccgttgcc tgcctgctgg aagtaccagg cgtgtggcgt tttatcaaca tccgtcgcaa
     8701 tctcatcaac acgcggcagc ggatgcagca ctttcatatt ggctttggcg ttgtggagat
     8761 cgctggcgcg aagaacaaac tgcgctttca cgttggcgta ctcggacggg tccagacgct
     8821 ctttttgcac gcgggtcatg tacaggatgt ctacttccgc catcacttct tcaatagagc
     8881 tgtgcagact ccatgcgatc cctttttcat cgagcatatc cagaatgtat tgcggcattg
     8941 ccagcgcgtc cggcgcgatg aagtaaaaac ggttgccatc gaacttcgct aacgcctgag
     9001 tcagggagtg aacggtgcgg ccatatttca ggtcaccaac cattgcgacg tggagattgt
     9061 ccagacgccc ctgggtttcc tgaatagtga ataagtccag caaggtttgc gtcggatgtt
     9121 ggttggagcc atcaccggca ttcagtaccg gtacattgcc ggaaaactcg gtggccaggc
     9181 gcgccgcacc ttcctgcgga tgacgcatca ctatcgcatc gacgtaagtg ctgataaccg
     9241 aaatggtatc ggccagcgtt tcgccctttt tacccagtga tgtattggcg ctgtcggaga
     9301 agcccaccac gctggcaccc aggcggtgca tagatgtttc gaaagagagg cgggtacggg
     9361 tagaggcttc gaagaaacag ctggcaatga ctttgtgctt caacagctct ggttgcgggt
     9421 ttgctttcag tttcgccgct gtcgccagca ccagattaag gtcatcgcga ctaaggtcgt
     9481 ttatggaaat gatatgtttc tgatatagcg gattagccat cttttatctc ctgacgcctg
     9541 ggcaaaaaaa agcccctcga ttgaggggct gggaatgggt gatcaacggg aagaaaaacg
     9601 gcaggccagc gtcttttttc agacgcggta agacaaaatg tcgaacacac tgaaccatac
     9661 atcctcccgg caaattgtcc ggcattatac tcatagccag aagcggtgca agcatttgat
     9721 gcaatatttt gtcagcgcaa acggtttatt tgaattaaaa gtcaaaatgt atgcattttt
     9781 atttgctttg attctggagg agaactgtcg ttcggtatca ataattgcag acatttcctg
     9841 cttttttctt tttcactttc acgcaatcag atatgcattt tattccattc atccgactta
     9901 tagggcggag tgtgaaaacg aacggctaac actattgctt acagctcagg gatgcgcgct
     9961 atcactttaa tttcaaaatc aaagcctgcc agccaggtaa cgcccaccgc cgtccagttt
    10021 ggataaggag gggcgctaaa tatttcattt ttgaccgtca tgatgtcttc aaattgtttt
    10081 tccggatcgg tatgaaagct cgtaacatca atgatatcgt caaaagtgca tcccgcagca
    10141 gccagggtcg catgcaaatt atcaaatgcc agtctgactt gttgctgaaa atcgggttct
    10201 ggtgttccgt cctctcgact tcctacttgc ccggaaacaa acagcaaatc gccggaacga
    10261 atagccgcag aataacgatg ctcagcatac agtgaatgtc ggccagcagg gaaaacagcg
    10321 gttctttcta ccatttggtt atcctcaaga tttacgacat gaacagaaga tttctcttta
    10381 ccgggagccg cttttagcgg acgacgtgag taaacaaaac ccagacatca tggataatgg
    10441 ctgggcttaa ttgagcgtag tcggttatgc gccaaacgcg ccatcaatgg tatgcatcgc
    10501 gccggtaaca aaactggctt ctggccctgc taaccatgcg accataccag cgacctcttc
    10561 cggttgccca tgtcttttga tagccatcaa actatgcaac atatcgcgca ttggcccgtt
    10621 ggcgggatta gcgtcggtat caattggccc tggctggacg acgttaatgg tgatcccacg
    10681 cggtccaaaa tcacgggcca gcccgcgcgc catgccttgc agggcagatt tgctggcggc
    10741 ataagcagcc atgcctgcaa caggcatacg atcgccattc acggagccga tgattaagat
    10801 gcgcccgcct tcgggcatct gccgggcggc ttcaacagag gcatgataag gagcatgaat
    10861 attgattttg aaaaggcgat caatatcgtc ggcatttaat tccagggcat cgccaaagac
    10921 gccaatacct gcatttacca ccaggatatc caatgcgccg ctcttacgaa cgacatcaat
    10981 gacagcgtct ctgtcagcac tatctgtaaa tactgctgtc gctccagtct cttgtgccag
    11041 atgttcagcg gcatctttcg accccgcata ggtgaatcgt acattggccc catcagtgac
    11101 gaaacgacgt acgatagcgg caccgattcc acgactgcca ccgaggatga gaactgtctt
    11161 acctgtaaaa gcgcccataa ggactccttg atttattatg taacatgcat tacaaaactg
    11221 ttttaacttt ctgtcaacaa gttttgtaat ggtcactaaa aaacaatctc gcgttccagg
    11281 tcgtcccaga cgtttcgctc ctgagcaggc agtctctgcg gcaaaagtgc tttttcacca
    11341 aaaaggtttc gatgctgtca gtgttgctga agttactgat tatcttggta ttaacccccc
    11401 gagcctctac gcagcttttg gcagtaaagt tgggttattt agccgtgtac tcaatgaata
    11461 tgtcggtacg gaagctattc cgcttgccga tattcttcgt gatgatcgtc cggtaggcga
    11521 gtgcctggtt gaggtattaa aagaagcggc gcgcagatat agccaaaacg gcggctgcgc
    11581 tggctgtatg gttcttgaag gtattcatag tcatgatcca caagcgcgtg atattgccgt
    11641 tcaatattat cacgccgcag aaacgaccat ttatgactat atcgccaggc ggcatccaca
    11701 aagcgcacaa tgtgtgactg attttatgag taccgtgatg tcagggcttt ctgcgaaggc
    11761 acgggagggg cactccatcg aacaactctg tgcaacagct gcactggcgg gggaagcgat
    11821 aaaaactatt ctcaaggagt gatgctggcc ttgacccgaa aggcgggaac gcgcctgccg
    11881 ataagttgtg ataagacaat aattcacgcg ttaaggctag cggaattgat catcttttcg
    11941 tataacggtg gaaatgaaac attgttttaa ttaaggagtg gaaaagatga tcatcggaaa
    12001 tattcataat cttcagccgt ggctacccca ggagttacgc caggcgattg agcatatcaa
    12061 agcgcacgtt acggcagaaa cgccaaaggg taagcacgat atcgaaggca atcgtctgtt
    12121 ttatcttatc tcggaagata tgaccgagcc gtacgaagct cgccgtgcgg agtatcatgc
    12181 ccgctatctc gacattcaga ttgtgttaaa aggtcaggaa ggcatgacct tcagcacgca
    12241 acctgcaggc acgccggata ccgactggtt agctgataaa gacatcgcat ttttgccgga
    12301 aggcgttgat gagaaaacag ttatccttaa tgaaggtgat tttgttgtgt tttatccggg
    12361 ggaagtgcat aaaccgctgt gcgcagtggg cgcgccagcc caggttcgca aagcagtagt
    12421 gaagatgctg atggcgtgat gacttttcgc cgtaaataac tccaggttta cggcgagttt
    12481 gtgaaaagag cgttttttga tatttttttg tgagtagaat ttgtaatgct tagacgttct
    12541 tattaactca aggagtccgt catggcaaaa atatcagatt tgaattgtta tcaacacatg
    12601 gcatcagccg acaatgttaa accaaaaaat gaagtcttaa atacctggtg tgtgggaatg
    12661 aatgattttg ctcgtaatgc cgaggggcag gatagcagaa ggaatattct atctcctgga
    12721 gcatttttgg agtttttggc aaaaatattt accctgggtt atgtggattt tagcaaacgc
    12781 tccaacgaag cgggtagaaa tatgatggct catattgagt cctcatctta tatcaaaaat
    12841 aatgatggca gtgaaataat gaattttgtt atgaataatc ctgaaggtga gcgtgcggat
    12901 tcatctaagg tgattataga aatttcactt tctactacca ctactatggg gactcgtcaa
    12961 ggacatacag ccattatatt tccacaatct gattttttga gttttcgtta tgaaggtaag
    13021 tcttttgaaa gaaaagatga tagttcatta catctgatta ctaacaaggt tctggcgtg
//
LOCUS       DAZKHF020000122        13001 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2201S31241
            SAMN43267800-rid25106413.denovo.123, whole genome shotgun sequence.
ACCESSION   DAZKHF020000122 DAZKHF020000000
VERSION     DAZKHF020000122.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267800
            Sequence Read Archive: SRR30307263
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 13001)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13001)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 13001)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350581.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350581.2
            Genome Coverage       :: 151x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:21:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,652
            CDSs (total)                      :: 5,557
            Genes (coding)                    :: 5,320
            CDSs (with protein)               :: 5,320
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 237
            CDSs (without protein)            :: 237
            Pseudo Genes (ambiguous residues) :: 0 of 237
            Pseudo Genes (frameshifted)       :: 102 of 237
            Pseudo Genes (incomplete)         :: 130 of 237
            Pseudo Genes (internal stop)      :: 52 of 237
            Pseudo Genes (multiple problems)  :: 41 of 237
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13001
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267800-rid25106413.denovo.123"
                     /serotype="O 26:H11"
                     /isolate="2201S31241"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-01"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            253..4713
                     /gene="gltB"
                     /locus_tag="ACHOSC_RS14135"
                     /old_locus_tag="pgaptmp_002827"
     CDS             253..4713
                     /gene="gltB"
                     /locus_tag="ACHOSC_RS14135"
                     /old_locus_tag="pgaptmp_002827"
                     /EC_number="1.4.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001742510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0015930 - glutamate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0006537 - glutamate biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate synthase large subunit"
                     /protein_id="HGU4548732.1"
                     /translation="MLYDKSLERDNCGFGLIAHIEGEPSHKVVRTAIHALARMQHRGA
                     ILADGKTGDGCGLLLQKPDRFFRIVAQERGWRLAKNYAVGMLFLNKDPELAAAARRIV
                     EEELQRETLSIVGWRDVPTNEGVLGEIALSSLPRIEQIFVNAPAGWRPRDMERRLFIA
                     RRRIEKRLEADKDFYVCSLSNLVNIYKGLCMPADLPRFYLDLADLRLESAICLFHQRF
                     STNTVPRWPLAQPFRYLAHNGEINTITGNRQWARARTYKFQTPLIPDLHDAAPFVNET
                     GSDSSSMDNMLELLLAGGMDIIRAMRLLVPPAWQNNPDMDPELRAFFDFNSMHMEPWD
                     GPAGIVMSDGRFAACNLDRNGLRPARYVITKDKLITCASEVGIWDYQPDEVVEKGRVG
                     PGELMVIDTRSGRILHSAETDDDLKSRHPYKEWMEKNVRRLVPFEDLPDEEVGSRELD
                     DDTLASYQKQFNYSAEELDSVIRVLGENGQEAVGSMGDDTPFAVLSSQPRIIYDYFRQ
                     QFAQVTNPPIDPLREAHVMSLATSIGREMNVFCEAEGQAHRLSFKSPILLYSDFKQLT
                     TMKEEHYRADTLDITFDVTKTTLEATVKELCDKAEKMVRSGTVLLVLSDRNIAKDRLP
                     VPAPMAVGAIQTRLVDQSLRCDANIIVETASARDPHHFAVLLGFGATAIYPYLAYETL
                     GRLVDTHAIAKDYRTVMLNYRNGINKGLYKIMSKMGISTIASYRCSKLFEAVGLHDDV
                     VGLCFQGAVSRIGGASFEDFQQDLLNLSKRAWLARKPISQGGLLKYVHGGEYHAYNPD
                     VVRTLQQAVQSGEYSDYQEYAKLVNERPATTLRDLLAITPGENAVNIADVEPASELFK
                     RFDTAAMSIGALSPEAHEALAEAMNSIGGNSNSGEGGEDPARYGTNKVSRIKQVASGR
                     FGVTPAYLVNADVIQIKVAQGAKPGEGGQLPGDKVTPYIAKLRYSVPGVTLISPPPHH
                     DIYSIEDLAQLIFDLKQVNPKAMISVKLVSEPGVGTIATGVAKAYADLITIAGYDGGT
                     GASPLSSVKYAGCPWELGLVETQQALVANGLRHKIRLQVDGGLKTGVDIIKAAILGAE
                     SFGFGTGPMVALGCKYLRICHLNNCATGVATQDDKLRKNHYHGLPFKVTNYFEFIARE
                     TRELMAQLGVTRLVDLIGRTDLLKELDGFTAKQQKLALSKLLETAEPHPGKALYCTEN
                     NPPFDNGLLNAQLLQQAKPFVDERQSKTFWFDIRNTDRSVGASLSGYIAQTHGDQGLA
                     ADPIKAYFNGTAGQSFGVWNAGGVELYLTGDANDYVGKGMAGGLIAIRPPVGSAFRSH
                     EASIIGNTCLYGATGGRLYAAGRAGERFGVRNSGAITVVEGIGDNGCEYMTGGIVCIL
                     GKTGVNFGAGMTGGFAYVLDESGDFRKRVNPELVEVLSVDDLAIHEEHLRGLITEHVQ
                     HTGSQRGEEILANWSTFATKFALVKPKSSDVKALLGHRSRSAAELRVQAQ"
     gene            4726..6144
                     /gene="gltD"
                     /locus_tag="ACHOSC_RS14140"
                     /old_locus_tag="pgaptmp_002828"
     CDS             4726..6144
                     /gene="gltD"
                     /locus_tag="ACHOSC_RS14140"
                     /old_locus_tag="pgaptmp_002828"
                     /EC_number="1.4.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001540975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate synthase subunit GltD"
                     /protein_id="HGU4548733.1"
                     /translation="MSQNVYQFIDLQRVDPPKKPLKIRKIEFVEIYEPFSEGQAKAQA
                     DRCLSCGNPYCEWKCPVHNYIPNWLKLANEGRIFEAAELSHQTNPLPEVCGRVCPQDR
                     LCEGSCTLNDEFGAVTIGNIERYINDKAFEMGWRPDMSGVKQTGKKVAIIGAGPAGLA
                     CADVLTRNGVKAVVFDRHPEIGGLLTFGIPAFKLEKEVMTRRREIFTGMGIEFKLNTE
                     VGRDVQLDDLLSDYDAVFLGVGTYQSMRGGLENEDADGVYAALPFLIANTKQLMGFGE
                     TRDEPFVSMEGKRVVVLGGGDTAMDCVRTSVRQGAKHVTCAYRRDEENMPGSRREVKN
                     AREEGVEFKFNVQPLGIEVNGNGKVSGVKMVRTEMGEPDAKGRRRAEIVAGSEHIVPA
                     DAVIMAFGFRPHNMEWLAKHSVELDSQGRIIAPEGSDNAFQTSNPKIFAGGDIVRGSD
                     LVVTAIAEGRKAADGIMNWLEV"
     gene            6328..7375
                     /gene="yhcG"
                     /locus_tag="ACHOSC_RS14145"
                     /old_locus_tag="pgaptmp_002829"
                     /pseudo
     CDS             6328..7375
                     /gene="yhcG"
                     /locus_tag="ACHOSC_RS14145"
                     /old_locus_tag="pgaptmp_002829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312120.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="YhcG family protein"
     gene            complement(7435..7899)
                     /gene="nanQ"
                     /locus_tag="ACHOSC_RS14150"
                     /old_locus_tag="pgaptmp_002830"
     CDS             complement(7435..7899)
                     /gene="nanQ"
                     /locus_tag="ACHOSC_RS14150"
                     /old_locus_tag="pgaptmp_002830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006974 - DNA damage response [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylneuraminate anomerase"
                     /protein_id="HGU4548734.1"
                     /translation="MMMGEVQSLPSAGLHPALQDALTLALAARPQEKAPGRYELQGDN
                     IFMNVMTFNTQSPVEKKAELHEQYIDIQLLLNGEERILFGMAGTARQCEEFHHEDDYQ
                     LCSTIDNEQAIILKPGMFAVFMPGEPHKPGCVVGEPGEIKKVVVKVKADLMA"
     gene            complement(7896..8771)
                     /gene="nanK"
                     /locus_tag="ACHOSC_RS14155"
                     /old_locus_tag="pgaptmp_002831"
     CDS             complement(7896..8771)
                     /gene="nanK"
                     /locus_tag="ACHOSC_RS14155"
                     /old_locus_tag="pgaptmp_002831"
                     /EC_number="2.7.1.60"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417689.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmannosamine kinase"
                     /protein_id="HGU4548735.1"
                     /translation="MTTLAIDIGGTKLAAALIGADGQIRDRRELPTPASQTPEALRDA
                     LSALVSPLQAHAQRVAIASTGIIRDGSLLALNPHNLGGLLHFPLVKTLEQLTNLPTIA
                     INDAQAAAWAEYQALEGDITDMVFITVSTGVGGGVVSGGKLLTGPGGLAGHIGHTLAD
                     PHGPVCGCGRTGCVEAIASGRGIAAAAQGELAGADARTIFTRAGQGDEQAQQLIHRSA
                     RTLARLIADIKATTDCQCVVVGGSVGLAEGYLALVETYLAQEPAAFHVDLLAAHYRHD
                     AGLLGAALLAQGEKL"
     gene            complement(8768..9457)
                     /gene="nanE"
                     /locus_tag="ACHOSC_RS14160"
                     /old_locus_tag="pgaptmp_002832"
     CDS             complement(8768..9457)
                     /gene="nanE"
                     /locus_tag="ACHOSC_RS14160"
                     /old_locus_tag="pgaptmp_002832"
                     /EC_number="5.1.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009385 - N-acylmannosamine-6-phosphate
                     2-epimerase activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006051 - N-acetylmannosamine metabolic
                     process [Evidence IEA];
                     GO_process: GO:0019262 - N-acetylneuraminate catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmannosamine-6-phosphate 2-epimerase"
                     /protein_id="HGU4548736.1"
                     /translation="MSLLAQLDQKIAANGGLIVSCQPVPDSPLDKPEIVAAMALAAEQ
                     AGAVAIRIEGVANLQATRAVVSVPIIGIVKRDLEDSPVRITAYIEDVDALAQAGADII
                     AIDGTDRPRPVPVETLLARIHHHGLLAMTDCSTPEDGLACQKLGAEIIGTTLSGYTTP
                     ETPEEPDLALVKTLSDAGCRVIAEGRYNTPAQAADAMRHGAWAVTVGSAITRLEHICQ
                     WYNTAMKKAVL"
     gene            complement(9505..10995)
                     /gene="nanT"
                     /locus_tag="ACHOSC_RS14165"
                     /old_locus_tag="pgaptmp_002833"
     CDS             complement(9505..10995)
                     /gene="nanT"
                     /locus_tag="ACHOSC_RS14165"
                     /old_locus_tag="pgaptmp_002833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312124.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sialic acid transporter NanT"
                     /protein_id="HGU4548737.1"
                     /translation="MSTTTQNIPWYRHLNRAQWRAFSAAWLGYLLDGFDFVLIALVLT
                     EVQGEFGLTTVQAASLISAAFISRWFGGLMLGAMGDRYGRRLAMVTSIVLFSAGTLAC
                     GFAPGYITMFIARLVIGMGMAGEYGSSATYVIESWPKHLRNKASGFLISGFSVGAVVA
                     AQVYSLVVPVWGWRALFFIGILPIIFALWLRKNIPEAEDWKEKHAGKAPVRTMVDILY
                     RGEHRIANIVMTLAAATALWFCFAGNLQNAAIVAVLGLLCAAIFISFMVQSTGKRWPT
                     GVMLMVVVLFAFLYSWPIQALLPTYLKTDLAYNPHTVANVLFFSGFGAAVGCCVGGFL
                     GDWLGTRKAYVCSLLASQLLIIPVFAIGGANVWVLGLLLFFQQMLGQGIAGILPKLIG
                     GYFDTDQRAAGLGFTYNVGALGGALAPIIGALIAQRLDLGTALASLSFSLTFVVILLI
                     GLDMPSRVQRWLRPEALRTHDAIDGKPFSGAVPFGSAKNDLVKTKS"
     gene            complement(11105..11998)
                     /gene="nanA"
                     /locus_tag="ACHOSC_RS14170"
                     /old_locus_tag="pgaptmp_002834"
     CDS             complement(11105..11998)
                     /gene="nanA"
                     /locus_tag="ACHOSC_RS14170"
                     /old_locus_tag="pgaptmp_002834"
                     /EC_number="4.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008747 - N-acetylneuraminate lyase
                     activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylneuraminate lyase"
                     /protein_id="HGU4548738.1"
                     /translation="MATNLRGVMAALLTPFDQQQALDKASLRRLVQFNIQQGIDGLYV
                     GGSTGEAFVQSLSEREQVLEIVAEEAKGKIKLIAHVGCVSTAESQQLAASAKRYGFDA
                     VSAVTPFYYPFSFEEHCDHYRAIIDSADGLPMVVYNIPALSGVKLTLDQINTLVTLPG
                     VGALKQTSGDLYQMEQIRREHPDLVLYNGYDEIFASGLLAGADGGIGSTYNIMGWRYQ
                     GIVKALKEGDIQTAQKLQTECNKVIDLLIKTGVFRGLKTVLHYMDVVSVPLCRKPFGP
                     VDEKYLPELKALAQQLMQERG"
     gene            complement(12120..12911)
                     /gene="nanR"
                     /locus_tag="ACHOSC_RS14175"
                     /old_locus_tag="pgaptmp_002835"
     CDS             complement(12120..12911)
                     /gene="nanR"
                     /locus_tag="ACHOSC_RS14175"
                     /old_locus_tag="pgaptmp_002835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417693.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator NanR"
                     /protein_id="HGU4548739.1"
                     /translation="MGLMNAFDSQTEDSSPAIGRNLRSRPLARKKLSEMVEEELEQMI
                     RRREFGEGEQLPSERELMAFFNVGRPSVREALAALKRKGLVQINNGERARVSRPSADT
                     IIGELSGMAKDFLSHPGGIAHFEQLRLFFESSLVRYAAEHATDEQIDLLAKALEINSQ
                     SLDNNAAFIRSDVDFHRVLAEIPGNPIFMAIHVALLDWLIAARPTVTDQALHEHNNVS
                     YQQHIAIVDAIRRHDPDEADRALQSHLNSVSATWHAFGQTTNKKK"
BASE COUNT         3084 a         3586 c         3514 g         2817 t
ORIGIN      
        1 catttgacct gtatcagctt tcccgataag ttggaaatcc gctggaagct ttctggatga
       61 gcagcctgct catcatattt atgcagtaat tgagatcccc tcttcaccgt attaaccgat
      121 gcgaaaagga caacaagggg gcgaatgcga ggcgcgcgta tgacacgcaa accccgtcgc
      181 cacgctcttt ctgtgcccgt gcgcagcggt tcggaagtgg ggttcccgca gagcctgggg
      241 gaggttcacg atatgttgta cgataaatcc cttgagaggg ataactgtgg tttcggcctg
      301 atcgcccaca tagaaggcga acctagccac aaggtagtgc gtactgcaat acacgcactg
      361 gcccgcatgc agcaccgtgg cgcgattctc gccgatggta aaaccggcga cggttgcggc
      421 ttgctgttac aaaaaccgga tcgctttttt cgcatcgttg cgcaggagcg cggctggcgt
      481 ttagcaaaaa actacgccgt cgggatgcta ttcctgaata aagatcctga actcgccgct
      541 gccgcacgcc gcatcgttga agaagaactg caacgcgaaa ccttgtcgat tgtgggctgg
      601 cgtgatgtcc ccactaacga aggcgtgctg ggtgaaatcg ccctctcctc tctgccacgc
      661 attgagcaaa tttttgtgaa cgccccggca ggctggcgtc cgcgcgatat ggagcgccgt
      721 ctgtttatcg cccgccgccg cattgaaaag cgtctcgaag ccgacaaaga cttctacgtc
      781 tgtagcctgt cgaatctggt gaacatctat aaaggtctgt gtatgccggc ggatctgccg
      841 cgcttttatc tggatcttgc ggacctgcgt ctggaatcgg ccatctgcct gttccaccag
      901 cgcttctcca ctaacaccgt accgcgctgg ccgctggcgc agccgttccg ctacctggcg
      961 cataacggtg aaatcaacac catcaccggt aaccgccaat gggcgcgtgc gcgtacctat
     1021 aaattccaga cgccgcttat ccctgatctg cacgacgccg caccgttcgt caacgaaacc
     1081 ggctctgact ccagttcgat ggataacatg ctggaactgc tgctggcagg cgggatggat
     1141 atcatccgcg ccatgcgtct attagtacca cccgcctggc agaacaaccc ggatatggac
     1201 ccggaactgc gtgccttctt tgactttaac tccatgcata tggagccgtg ggatggcccg
     1261 gcgggcatcg tgatgtccga tggtcgcttc gctgcctgta acctcgaccg taacggtctg
     1321 cgtccggcac gctacgtcat taccaaagac aagctcatca cctgcgcctc tgaagtcggt
     1381 atctgggatt atcagcctga cgaagtggtc gaaaaaggcc gcgtcgggcc aggcgaactg
     1441 atggtgatcg acacccgcag tgggcgtatt ctgcactcgg cagaaaccga tgacgatctg
     1501 aaaagccgcc atccatataa agagtggatg gagaaaaacg tccgccgact ggtaccgttt
     1561 gaagatctgc ccgatgaaga agtgggtagc cgcgaactgg acgacgacac gcttgccagc
     1621 taccagaaac agtttaacta cagcgcggaa gagctggact ccgtaattcg cgtactgggc
     1681 gaaaacggtc aggaagcggt cggttcgatg ggcgatgata ccccattcgc cgtgctctcc
     1741 agtcagccgc gcattattta cgactacttc cgccagcagt ttgcccaggt gactaacccg
     1801 ccaatcgacc cgctgcgtga agcgcatgtt atgtcgctcg ccaccagtat cggtcgtgaa
     1861 atgaacgtct tttgcgaagc agagggccag gcgcaccgtt taagctttaa atcgccgatt
     1921 ctgctctact ccgatttcaa acagctcacg acgatgaaag aggagcacta ccgcgcagat
     1981 acgctggata tcacctttga cgtcactaaa accacgctcg aagcgacagt caaagagctg
     2041 tgcgacaaag ccgaaaaaat ggtacgtagc ggcaccgtgc tgctggtgct ctccgaccgg
     2101 aatatcgcta aagatcgcct gccggttcca gccccgatgg cggttggcgc gatccagacc
     2161 cgtctggtcg atcaaagcct gcgttgcgat gccaacatca tcgtcgaaac cgccagcgcc
     2221 cgcgatccgc accacttcgc cgtgttgctg ggcttcggcg cgacggctat ttatccatac
     2281 cttgcctatg aaacgctggg ccgcctggta gacacccatg cgattgccaa agattatcgt
     2341 accgtgatgc tcaactaccg taacggcatc aacaaaggct tgtacaaaat catgtccaaa
     2401 atgggcatct ccaccatcgc ctcttaccgc tgctcgaaac tgtttgaagc ggtcggtctg
     2461 cacgatgatg tagtgggcct gtgcttccag ggggcggtca gccgcattgg tggggcgagc
     2521 tttgaagact tccagcagga tctgctgaac ctgtcgaaac gtgcctggct ggcgcgtaag
     2581 cccatcagcc agggcggcct gctgaaatac gtccacggcg gcgaatacca cgcttacaac
     2641 ccggacgtgg tgcgcacgct gcaacaggcg gtacaaagcg gcgagtacag cgactatcag
     2701 gaatacgcga agctggttaa tgagcgtccg gcaaccacgc tgcgcgatct gctggcaatt
     2761 acgccgggtg aaaacgcggt caacattgct gatgttgaac cggcaagcga actgtttaaa
     2821 cgctttgata ccgccgcgat gtctatcggc gcgttaagcc cggaagccca cgaggcgctg
     2881 gcggaagcga tgaatagcat cggcggtaac tcgaactccg gtgaaggcgg cgaagacccg
     2941 gcgcgctatg gcactaacaa agtgtcgcgc atcaagcagg tggcttctgg tcgcttcggc
     3001 gttactccgg cgtatctggt caatgccgac gtcattcaga ttaaagtcgc ccagggcgcg
     3061 aagccgggcg aaggcggtca gttgccgggc gacaaagtca ctccttacat cgccaaactg
     3121 cgctattcgg tgcccggagt gacgctgatc tccccgccgc cgcaccacga tatctactct
     3181 atcgaggact tagcgcagct cattttcgac ctcaagcagg ttaacccgaa agcgatgatc
     3241 tccgtgaagc tggtttccga accgggcgta ggcaccatcg cgactggcgt ggcaaaagct
     3301 tatgccgatt taatcaccat cgcaggctat gacggtggca ccggcgcaag cccgctgtcg
     3361 tcagtgaaat acgcaggttg cccgtgggag ctggggcttg ttgaaaccca gcaggcgctg
     3421 gttgccaatg gtctgcgtca taagatccgt ttgcaggtcg atggcggcct gaaaaccggt
     3481 gttgatatca tcaaagcggc gattctcggc gcagaaagct tcggcttcgg cactggcccg
     3541 atggtggcgc tcggctgtaa atatctacgt atttgccatc tgaacaactg cgcaacgggt
     3601 gtagcaactc aggatgacaa actgcgtaag aaccactatc acggcctgcc gttcaaggtt
     3661 accaattact ttgaatttat tgcccgtgaa acccgcgagc tgatggcaca gctgggcgta
     3721 acgcgtctgg tggatctgat tggtcgcacc gacctgctga aagaactgga cggtttcacc
     3781 gccaaacagc aaaaactggc gctgtcgaag ctgctggaga ccgccgaacc gcatccaggt
     3841 aaggcactct actgcaccga aaacaaccca ccgtttgata acggcctgct gaacgcgcag
     3901 ttgctgcaac aggcgaaacc gtttgtcgat gaacgccaga gcaaaacctt ctggttcgat
     3961 attcgcaaca ccgaccgttc tgtcggcgcg tcgctttcag gctatatcgc ccagacgcac
     4021 ggcgatcagg ggctggcagc cgatcctatc aaagcgtact tcaacggcac cgcaggccag
     4081 agtttcggtg tgtggaacgc gggcggcgtg gaactgtacc tgaccgggga tgccaacgac
     4141 tatgtcggta aaggcatggc gggcggctta atcgccattc gtcctccggt tggttccgcc
     4201 ttccgcagcc atgaagcaag tattatcggc aacacctgcc tgtatggcgc gaccggtggt
     4261 cgtctgtatg ccgcaggccg cgcgggtgaa cgtttcggcg tgcggaactc cggtgctatc
     4321 accgtggtag aaggcattgg cgacaacggc tgtgaatata tgacgggcgg tattgtctgt
     4381 attctgggta aaaccggcgt taacttcggt gcgggcatga ccggcggctt cgcttacgtt
     4441 ctcgatgaaa gcggtgattt ccgcaaacgc gttaacccgg aactggtcga ggtcttaagc
     4501 gttgacgatc tggcgatcca tgaagagcat ctgcgcggtc ttatcaccga gcatgttcag
     4561 catactggct ctcagcgcgg tgaagagatt ctggcgaact ggtcaacctt cgccactaaa
     4621 tttgcgctgg ttaaaccgaa gtccagtgat gtaaaagcac tgctgggtca tcgtagtcgt
     4681 agcgcagcag agttgcgcgt gcaggcgcag taaggggtag caacaatgag tcagaatgtt
     4741 tatcaattta tcgacctgca gcgcgttgat ccgccaaaga aaccgctgaa gatccgcaaa
     4801 attgagtttg ttgaaattta cgagccgttt tccgaaggcc aggccaaagc gcaggctgac
     4861 cgctgcctgt cgtgcggcaa cccatactgc gagtggaaat gtccggtaca caactacatc
     4921 ccgaactggc tgaagctcgc caacgagggg cgtatttttg aagcggcgga actgtcgcac
     4981 cagaccaacc ccctgccgga agtttgcgga cgagtctgcc cgcaagaccg tctgtgcgaa
     5041 ggttcctgca ctctgaacga tgagtttggc gcggtgacca tcggcaacat tgagcgttat
     5101 atcaatgata aagcgttcga aatgggctgg cgtccggata tgtccggcgt gaagcaaacc
     5161 ggtaaaaaag tggcgattat cggcgcaggc ccggcaggtc tggcgtgtgc ggatgtcctg
     5221 acgcgtaacg gcgtaaaagc cgttgtcttc gaccgtcatc cagaaattgg cgggctgctg
     5281 accttcggta ttccggcctt caagctggaa aaagaggtaa tgacgcgtcg ccgtgaaatc
     5341 ttcaccggca tgggtattga attcaaactc aataccgaag tgggccgcga cgtgcagctg
     5401 gacgatctgc tgagtgatta cgatgccgtc ttccttggcg tcgggactta tcagtcaatg
     5461 cgcggcgggc tggaaaacga agacgccgat ggcgtgtacg cagcgctgcc gttccttatc
     5521 gccaacacca aacagttaat gggctttggt gaaacccgcg acgaaccgtt cgtcagcatg
     5581 gaaggcaaac gcgtggtggt ccttggcggt ggcgacactg cgatggactg cgtgcgtacg
     5641 tccgtgcgcc agggagcgaa gcacgttacc tgtgcctatc gtcgtgatga agagaacatg
     5701 ccgggttccc gccgcgaagt gaaaaacgcg cgggaagaag gcgtagagtt caaattcaac
     5761 gtccagccac tggggattga agtgaacggt aacggtaaag tcagcggcgt aaaaatggta
     5821 cgtaccgaaa tgggcgaacc ggatgccaaa ggccgtcgcc gcgcggagat cgttgcaggt
     5881 tccgaacata tcgttccggc agatgcagtg atcatggcgt ttggtttccg tccacacaac
     5941 atggaatggc tggcaaaaca cagcgtcgag ctggattcac aaggccgcat catcgccccg
     6001 gaaggcagcg acaacgcctt ccagaccagc aacccgaaaa tctttgctgg cggcgatatc
     6061 gtccgtggtt ccgatctggt ggtaaccgct attgccgaag gtcgtaaggc ggcagacggt
     6121 attatgaact ggctggaagt ttaagctctg tcccagcgcc gcctcctcag aaaagagtcg
     6181 gcgctgaaat tctcctttct ttctggaaag cgcctcgcat aatctgaaga aaaccaatgt
     6241 aatacgctca tctggattgt taccggtaga tgcccggcgt atagctgatt ccattattca
     6301 acgcagcgcc atataaaggg acagataatg gaatcgctct cagagagaac ctcaacaggc
     6361 taccagcaaa tccacgacgg tattattcat ctggtcgata gcgcccggac ggaaacggta
     6421 cgtagcgtta acgcgctgat gaccgcgaca tactgggaaa ttggccgacg aattgtcgaa
     6481 tttgaacaag gtggcgaggc cagggctgcg tatggtgcgc agctaatcaa gcgactatca
     6541 aaggatttaa gtctaaggta taagcgtggg ttctctgcaa aaaacttacg ccaaatgagg
     6601 cttttttacc tcttttttca acatgttgaa attcgccaga cagtgtctgg cgaattaaca
     6661 cctgccctgg tccacctacg tccgtttact ttccgttaaa aacgctgacg cccgcagctt
     6721 ttatgaaaaa gagacgctcc gctgtggctg gtctgttcgc cagctagaac ggcaaattgc
     6781 gacccaattt tatgagcgaa cactactgtc acatgacaaa tcagccatgc tgcaacaaca
     6841 cgctcctacc gagacgcata ttcttccgca acaggcgata cgcgatccct ttgtgctcga
     6901 atttctggaa ttgaaagatg aatattcaga atctgatttc gaggaggcgc tgatcaacca
     6961 cctgatggat ttcatgctgg aacttgggga tgattttgcc tttgttggtc ggcagcgaag
     7021 gttacgcatt gatgacaact ggtttcgtgt cgatctgctg tttttccacc gccgtttacg
     7081 ctgcctacta atcgtcgatc taaaagtggg caaattcagc tatagcgatg ccggacagat
     7141 gaatatgtat ctcaactacg ccaaagagca ctggacgcta ccggatgaaa atccgcccat
     7201 cggtctggtt ctctgtgcag agaaaggagc cggagaagca cattatgctc tggcaggttt
     7261 gcctaacacc gttctggcaa gcgaatataa gatgcaacta cctgatgaga aacgactcgc
     7321 agatgaactc gttcgaacac aggcggtgct agaggaaggc tatagacgcc gttaatttca
     7381 gcggtttcga attctgcaga cagtgtctgc agaatcttta aaacgaacta agttttaagc
     7441 cattaaatca gccttaacct tcaccacaac ctttttaatc tctccaggct cgccgacaac
     7501 gcatcctggt ttatgcggtt cacctggcat aaacacggcg aacattcccg gttttaagat
     7561 gatggcttgc tcgttatcaa tggtgctgca aagctgataa tcatcctcat ggtggaactc
     7621 ttcacactgg cgcgcagtgc ctgccatgcc aaacagaatc cgttcctcac cgtttaataa
     7681 cagttggata tcaatgtatt gctcgtgcaa ttccgctttt ttctcgacgg gcgattgagt
     7741 gttaaacgtc atgacattca taaagatatt gtcgccctgt aattcgtaac gacccggcgc
     7801 tttttcttgc ggtctggcag ctaatgccag cgttaacgcg tcctgtaacg caggatgtaa
     7861 cccagcagac ggtaatgact gtacttcacc catcatcata atttttctcc ctgggccaac
     7921 agcgcagccc caagtaaacc tgcatcgtgg cggtagtgcg ccgccagtaa atcaacatga
     7981 aatgccgctg gctcctgcgc cagatacgtt tccaccagcg ccagataccc ttctgccagc
     8041 ccaacgctgc caccgaccac cacgcactgg caatcagttg tggctttaat atcagcgatc
     8101 agccttgcaa gcgtacgtgc ggagcggtga atcagctgct gcgcctgctc gtcgccctgc
     8161 ccggcgcgtg tgaaaatagt tctcgcatcc gcgcccgcca gttcaccctg cgctgccgcc
     8221 gcaatgccgc gaccagaagc aatcgcctcc acgcaacccg tgcgtccaca gccacagacc
     8281 gggccgtgtg gatcggcaag cgtatgcccg atgtgccccg ccagaccgcc agggccggta
     8341 agcagtttgc cgccgctcac tacaccgccg ccgacgccag tggaaacggt gataaagacc
     8401 atatcggtta tatcgccttc cagcgcctga tactctgccc atgctgcggc ctgcgcgtcg
     8461 ttaatggcaa tggtcggcaa attggtaagt tgttccagcg ttttgactaa cggaaagtgt
     8521 agcaatccac cgagattatg cggattcagc gccagcaaac tgccgtcgcg aataattccg
     8581 gttgaagcga tggcaacccg ctgcgcatga gcttgcaacg gagagactaa tgcggataag
     8641 gcatcacgca aggcttctgg tgtctggctg gctggcgtag gaagttcacg acgatcgcgg
     8701 atctgcccgt cagcgccaat cagcgcggcg gcaagtttag taccgccgat atcaatcgcc
     8761 agcgtggtca tagcaccgcc tttttcatcg ctgtgttgta ccactgacaa atgtgttcaa
     8821 gacgcgtaat ggcagaaccg accgtcaccg cccacgcgcc gtggcgcatc gcatctgccg
     8881 cctgagcagg cgtgttgtaa cgcccttcgg caatcaccct acatccggcg tcgctcaacg
     8941 ttttcaccag cgccagatcc ggctcttctg gcgtttcagg cgtggtatag ccagaaagcg
     9001 tagtgccaat aatttcggct cccagctttt ggcatgccag gccgtcttcc ggcgttgagc
     9061 agtcggtcat cgccagtaaa ccgtgatggt gaatacgtgc cagcagcgtt tcaacaggca
     9121 ccggacgcgg gcggtcggtg ccgtcaatgg cgataatgtc cgcgcccgcc tgcgccagcg
     9181 catcaacatc ttcaatatag gccgtgatgc gtaccggaga atcctccaga tcgcgtttca
     9241 caattccaat aatcggcacg ctcaccaccg cacgcgtggc ttgcagattt gccacacctt
     9301 caatgcgaat ggcaaccgcg cccgcctgtt ctgccgctaa tgccatggcg gcgacgattt
     9361 cgggtttatc gagcgggctg tccggaaccg gctggcagga gacaatcagg ccaccgttag
     9421 cagcgatttt ttgatccagt tgtgcaagta acgacataca tcttccctta gcgaaaggcc
     9481 cggtacatag accgggcaac aggattaact tttggttttg actaaatcgt ttttggcgct
     9541 gccaaatggc acggcaccgc taaatggttt gccgtcgata gcgtcatgag tacgcaatgc
     9601 ttccgggcgc agccaacgct gaacgcggga aggcatatcc agaccaatca gcaggatcac
     9661 cacaaatgtc agactgaacg atagcgatgc cagcgcagta cccagatcca gacgttgagc
     9721 gatcaacgcg ccgatgattg gggccagtgc gccgcccaat gcaccaacgt tgtaggtaaa
     9781 gcccaggccc gctgcacgct ggtcggtatc gaaataaccg ccaatcagtt tcggtaagat
     9841 cccggcgatc ccttgtccaa gcatttgctg gaagaacagt aacagaccga gcacccagac
     9901 gtttgcgccg ccaatcgcaa ataccggaat aatcaacagc tgcgaggcca acaggctaca
     9961 aacgtacgct ttgcgggttc ccagccagtc accgaggaag ccgccgacac agcaccccac
    10021 cgcagcccca aagccgctaa agaacagcac attggcgaca gtatgcgggt tataagccag
    10081 atcggttttc agatacgttg gcagcagcgc ctgaatcggc catgagtaga ggaaagcaaa
    10141 caacacgacc accatcagca ttacgcccgt tggccagcgt ttacccgtac tttgcaccat
    10201 aaagctgata aagattgcgg cgcataacag cccaagaaca gcgacgatcg cggcattttg
    10261 caggttaccg gcgaagcaaa accacagcgc agtagccgcc gccagtgtca ttacgatatt
    10321 ggcaatgcga tgttcaccac ggtagagaat atctaccatc gtgcgtactg gtgctttacc
    10381 tgcatgtttc tctttccagt cttccgcttc cgggatgttt ttacgcagcc agagagcaaa
    10441 gatgataggc aaaatgccga taaagaacag cgcacgccag ccccagaccg gaaccaccag
    10501 gctatagacc tgagcggcaa cgacggcccc cacagagaag cctgaaatca gaaaaccact
    10561 ggctttgtta cgcagatgtt ttggccagct ttcaatgaca taggtggcgc tggaaccgta
    10621 ttcacccgcc atccccatgc cgatgaccag acgcgcgata aacatggtga tgtagcctgg
    10681 cgcaaagccg caggccagcg tcccggccga gaagagaacg atgctggtga ccattgccag
    10741 acgacgcccg tagcggtcac ccatagcgcc gagcatcagg ccgccgaacc agcgagagat
    10801 aaaggctgcg gagatcagac ttgccgcctg caccgtcgtc agcccgaatt caccttgtac
    10861 ttcggtgagt accagggcga ttaaaacgaa atcaaaaccg tcaagcagat atcccaacca
    10921 ggcagcggaa aatgcccgcc attgtgcacg attgagatgg cgataccacg ggatattctg
    10981 ggttgtagta ctcattgtga gtctcccgcg gtgggcgatg cccacacgct ttggtatgaa
    11041 aattgtaggg tacagatgcg tttatttccc ctcacccggt aggggcgagc gaggggaaaa
    11101 caactcaccc gcgctcttgc atcaactgct gggccagcgc cttcagttct ggcagatatt
    11161 tttcatctac cggtccaaac ggtttgcggc acagcggcac agaaacgaca tccatataat
    11221 ggaggacagt tttcaggccg cggaatacgc ccgttttgat cagtaaatca atgactttat
    11281 tgcattcagt ttgcagtttc tgcgcggtct ggatatcgcc ttctttcagc gccttaacga
    11341 tcccctgata gcgccagccc atgatgttgt aggtactacc gataccacca tcagcgcccg
    11401 ccagcagacc agaggcgaag atttcgtcgt aaccgttata gagcacaaga tcaggatgtt
    11461 cacgacggat ctgctccatc tgatagagat cgccagaagt ctgtttcagc gcaccgacgc
    11521 caggcaacgt aaccagggtg ttgatctgat ccagggtcag ttttacccca ctcagggctg
    11581 gaatgttgta caccaccatc ggcaaaccat ccgctgaatc aataattgcc cgatagtgat
    11641 cgcagtgttc ttcaaagctg aaaggatagt agaacggcgt gacggcggag acggcatcga
    11701 agccataacg tttagccgat gccgcaagtt gttggctttc ggcggtgctg acgcaaccga
    11761 cgtgggcgat gagtttaatc ttacctttcg cctcttcggc gacgatttcc agtacctgtt
    11821 cacgctcgga aaggctttgt acaaaggcct cgccggtcga accacccacg tataaaccgt
    11881 cgatgccctg ctgaatattg aactgaacca ggcgacgcag actcgcttta tccagtgctt
    11941 gttgttgatc aaaaggagtc aggagtgcag ccattacgcc acgtaaattc gttgccataa
    12001 atacctctga agtgatgctt gtctgataaa cgatatacct ttatacctgt tataccagat
    12061 caattaagca acaccccata cagaaagctt ataatgcgat ctgcttcact aaagtggcat
    12121 tatttctttt tgttggtggt ctgaccgaaa gcgtgccagg tagcagagac gctgttgaga
    12181 tgcgattgca acgcacgatc ggcttcgtca ggatcatgac ggcggatcgc atcaacgatc
    12241 gcaatatgct gttgataact aacgttgtta tgttcgtgca gtgcctgatc ggtaaccgtt
    12301 gggcgtgcgg caataagcca gtcgagcagg gcaacgtgga tcgccatgaa gattgggtta
    12361 ccggggatct ccgccagcac gcggtggaaa tcaacgtctg aacgaatgaa tgccgcgttg
    12421 ttatccagcg actgactgtt aatttccagt gcttttgcca gcaaatcgat ttgctcatcg
    12481 gtggcatgtt cagccgcata gcgcaccagg ctggattcaa agaacagacg taattgttcg
    12541 aaatgggcaa tcccaccagg atgagaaagg aaatctttcg ccatgccgga aagctcgccg
    12601 atgatggtgt ccgcagaagg acgtgagacg cgggcacgtt cgccgttgtt gatttgcacc
    12661 agacctttgc gttttaacgc tgccagcgct tcgcgcacag aaggacgccc gacgttaaag
    12721 aacgccatca gttcgcgttc agacggtaat tgttcacctt cgccaaattc acgacggcgg
    12781 atcatctgtt ccagctcttc ttccaccatt tcggagagtt ttttacgcgc cagcgggcgg
    12841 ctacgcaagt tgcgaccaat tgcaggtgaa gaatcttcgg tttgcgaatc aaatgcgttc
    12901 ataaggccca ttctgtaagg tcagtgtgat taacatcatc agtgacatcc tatcacagga
    12961 ttgaaagtag gggaaaatgg caggtttttc tctttgtgcc t
//
LOCUS       DAZKHF020000123        12914 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2201S31241
            SAMN43267800-rid25106413.denovo.124, whole genome shotgun sequence.
ACCESSION   DAZKHF020000123 DAZKHF020000000
VERSION     DAZKHF020000123.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267800
            Sequence Read Archive: SRR30307263
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 12914)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12914)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 12914)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350581.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350581.2
            Genome Coverage       :: 151x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:21:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,652
            CDSs (total)                      :: 5,557
            Genes (coding)                    :: 5,320
            CDSs (with protein)               :: 5,320
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 237
            CDSs (without protein)            :: 237
            Pseudo Genes (ambiguous residues) :: 0 of 237
            Pseudo Genes (frameshifted)       :: 102 of 237
            Pseudo Genes (incomplete)         :: 130 of 237
            Pseudo Genes (internal stop)      :: 52 of 237
            Pseudo Genes (multiple problems)  :: 41 of 237
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12914
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267800-rid25106413.denovo.124"
                     /serotype="O 26:H11"
                     /isolate="2201S31241"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-01"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            145..480
                     /gene="yjdM"
                     /locus_tag="ACHOSC_RS14180"
                     /old_locus_tag="pgaptmp_002836"
     CDS             145..480
                     /gene="yjdM"
                     /locus_tag="ACHOSC_RS14180"
                     /old_locus_tag="pgaptmp_002836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein YjdM"
                     /protein_id="HGU4548740.1"
                     /translation="MSLPHCPKCNSEYTYEDNGMYICPECAYEWNDAEPAQESDELIV
                     KDANGNLLADGDSVTIIKDLKVKGSSSMLKIGTKVKNIRLVEGDHNIDCKIDGFGPMK
                     LKSEFVKKN"
     gene            640..1083
                     /gene="yjdN"
                     /locus_tag="ACHOSC_RS14185"
                     /old_locus_tag="pgaptmp_002837"
     CDS             640..1083
                     /gene="yjdN"
                     /locus_tag="ACHOSC_RS14185"
                     /old_locus_tag="pgaptmp_002837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family metalloprotein YjdN"
                     /protein_id="HGU4548741.1"
                     /translation="MSLSPYLSFAGNCADAIAYYQRTLGAELLYKISFGEMPKSAQDS
                     AENCPSGMQFPDTAIAHANVRIAGSDIMMSDAIPSGKASYSGFTLVLDSQQVEEGKRW
                     FDNLAANGKIEMAWQETFWAHGFGKVTDKFGVPWMINVVKQQPTQ"
     gene            1216..2004
                     /gene="phnC"
                     /locus_tag="ACHOSC_RS14190"
                     /old_locus_tag="pgaptmp_002838"
     CDS             1216..2004
                     /gene="phnC"
                     /locus_tag="ACHOSC_RS14190"
                     /old_locus_tag="pgaptmp_002838"
                     /EC_number="7.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709835.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate ABC transporter ATP-binding protein"
                     /protein_id="HGU4548742.1"
                     /translation="MQTIIRVEKLAKTFNQHQALHAVDLNIHHGEMVALLGPSGSGKS
                     TLLRHLSGLITGDKSAGSHIELLGRTVQREGRLARDIRKSRANTGYIFQQFNLVNRLS
                     VLENVLIGALGSTPFWRTCFSWFTGEQKQRALQALTRVGMVHFAHQRVSTLSGGQQQR
                     VAIARALMQQAKVILADEPIASLDPESARIVMDTLRDINQNDGITVVVTLHQVDYALR
                     YCERIVALRQGHVFYDGSSQQFDNERFDHLYRSINRIEENAKAA"
     gene            2029..3045
                     /gene="phnD"
                     /locus_tag="ACHOSC_RS14195"
                     /old_locus_tag="pgaptmp_002839"
     CDS             2029..3045
                     /gene="phnD"
                     /locus_tag="ACHOSC_RS14195"
                     /old_locus_tag="pgaptmp_002839"
                     /EC_number="7.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_process: GO:0015716 - organic phosphonate transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate ABC transporter substrate-binding
                     protein"
                     /protein_id="HGU4548743.1"
                     /translation="MNAKIIASLAFTSMFSLSTLLSPAHAEEQEKALNFGIISTESQQ
                     NLKPQWTPFLQDMEKKLGVKVNAFFAPDYAGIIQGMRFNKVDIAWYGNLSAMEAVDRA
                     NGQVFAQTVAADGSPGYWSVLIVNKDSPINNLNDLLAKRKDLTFGNGDPNSTSGFLVP
                     GYYVFAKNNISASDFKRTVNAGHETNALAVANKQVDVATNNTENLDKLKTSAPEKLKE
                     LKVIWKSPLIPGDPIVWRKNLSETTKDKIYDFFMNYGKTPEEKAVLERLGWAPFRASS
                     DLQLVPIRQLALFKEMQGVKSNKGLNEQDKLAKTTEIQAQLDDLDRLNNALSAMSSVS
                     KAVQ"
     gene            3151..3930
                     /gene="phnE"
                     /locus_tag="ACHOSC_RS14200"
                     /old_locus_tag="pgaptmp_002840"
     CDS             3151..3930
                     /gene="phnE"
                     /locus_tag="ACHOSC_RS14200"
                     /old_locus_tag="pgaptmp_002840"
                     /EC_number="7.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313113.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015416 - ABC-type phosphonate transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015716 - organic phosphonate transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate ABC transporter, permease protein
                     PhnE"
                     /protein_id="HGU4548744.1"
                     /translation="MQTITIAPPKRSWFSLLSWAVVLAVLVVSWQGAEMAPLTLIKDG
                     GNMATFAADFFPPDFSQWQDYLTEMAVTLQIAVWGTALAVVLSIPFGLMSAENLVPWW
                     VYQPVRRLMDACRVINEMVFAMLFVVAVGLGPFAGVLALFIHTTGVLSKLLSEAVEAI
                     EPGPVEGIRATGANKLEEILYGVLPQVMPLLISYSLYRFESNVRSATVVGMVGAGGIG
                     VTLWEAIRGFQFQQTCALMVLIIVTVSLLDFLSQRLRKHFI"
     gene            3951..4676
                     /gene="phnF"
                     /locus_tag="ACHOSC_RS14205"
                     /old_locus_tag="pgaptmp_002841"
     CDS             3951..4676
                     /gene="phnF"
                     /locus_tag="ACHOSC_RS14205"
                     /old_locus_tag="pgaptmp_002841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate metabolism transcriptional regulator
                     PhnF"
                     /protein_id="HGU4548745.1"
                     /translation="MHLSTHPTSYPTRYQEIAAKLEQELRQHYRCGDYLPAEQQLAAR
                     FEVNRHTLRRAIDQLVEKGWVQRRQGVGVLVLMRPFDYPLNAQARFSQNLLDQGSHPT
                     SEKLLSVLRPASGHVADALGITEGENVIHLRTLRRVNGVALCLIDHYFADLTLWPTLQ
                     RFDSGSLHDFLREQTGIALRRSQTRISARRAQAKECQRLEIPNMSPLLCVRTLNHRDG
                     ESSPAEYSVSLTRADMIEFTMEH"
     gene            4677..5129
                     /gene="phnG"
                     /locus_tag="ACHOSC_RS14210"
                     /old_locus_tag="pgaptmp_002842"
     CDS             4677..5129
                     /gene="phnG"
                     /locus_tag="ACHOSC_RS14210"
                     /old_locus_tag="pgaptmp_002842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015716 - organic phosphonate transport
                     [Evidence IEA];
                     GO_process: GO:0019634 - organic phosphonate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate C-P lyase system protein PhnG"
                     /protein_id="HGU4548746.1"
                     /translation="MHADTATRQHWMSVLAHSQPAELAARLKALNITADYEVIRAAET
                     GLVQIQARMGGTGERFFAGDATLTRAAVRLTDGTLGYSWVLGRDKQHAERCALIDALM
                     QQSRYFQNLSETLIAPLDADRMARIAARQAEVNASRVDFFTMVRGDNA"
     gene            5126..5710
                     /gene="phnH"
                     /locus_tag="ACHOSC_RS14215"
                     /old_locus_tag="pgaptmp_002843"
     CDS             5126..5710
                     /gene="phnH"
                     /locus_tag="ACHOSC_RS14215"
                     /old_locus_tag="pgaptmp_002843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0019634 - organic phosphonate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate C-P lyase system protein PhnH"
                     /protein_id="HGU4548747.1"
                     /translation="MTLETAFMLPVQDAQHSFRRLLKAMSEPGVIVALHQLKRGWQPL
                     NIATTSVLLTLADNDTPVWLAAPLSNDIVSQSLRFHTNAPLVSQPEQATFAVTDEAIS
                     SEQLNALSTGTAVAPEAGATLILQVASLSGGRMLRLTGAGIAEERMIAPQLPECILHE
                     LTERPHPFPLGIDLILTCGERLLAIPRTTHVEVC"
     gene            5710..6774
                     /gene="phnI"
                     /locus_tag="ACHOSC_RS14220"
                     /old_locus_tag="pgaptmp_002844"
     CDS             5710..6774
                     /gene="phnI"
                     /locus_tag="ACHOSC_RS14220"
                     /old_locus_tag="pgaptmp_002844"
                     /EC_number="2.7.8.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-D-ribose 1-methylphosphonate
                     5-triphosphate synthase subunit PhnI"
                     /protein_id="HGU4548748.1"
                     /translation="MYVAVKGGEKAIDAAHALQESRRRGDTDLPELSVAQIEQQLNLA
                     VDRVMTEGGIADRELAALALKQASGDNVEAIFLLRAYRTTLAKLAVSEPLDTTEMRLE
                     RRISAVYKDIPGGQLLGPTYDYTHRLLDFTLLANGEAPTLTTADSEQQPSPHVFSLLA
                     RQGLVKFEEDSGAQPDDITRTPPVYPCSRSSRLQQLMRGDEGYLLALAYSTQRGYGRN
                     HPFAGEIRSGYIDVSIVPEELGFAVNVGELLMTECEMVNGFIDPPDEPPHFTRGYGLV
                     FGMSERKAMAMALVDRALQAPEYGEHATGPAQDEEFVLAHADNVEAAGFVSHLKLPHY
                     VDFQAELELLKRLQQEKNHG"
     gene            6767..7612
                     /gene="phnJ"
                     /locus_tag="ACHOSC_RS14225"
                     /old_locus_tag="pgaptmp_002845"
     CDS             6767..7612
                     /gene="phnJ"
                     /locus_tag="ACHOSC_RS14225"
                     /old_locus_tag="pgaptmp_002845"
                     /EC_number="4.7.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-D-ribose 1-methylphosphonate 5-phosphate
                     C-P-lyase PhnJ"
                     /protein_id="HGU4548749.1"
                     /translation="MANLSGYNFAYLDEQTKRMIRRAILKAVAIPGYQVPFGGREMPM
                     PYGWGTGGIQLTASVIGESDVLKVIDQGADDTTNAVSIRNFFKRVTGVNTTERTDDAT
                     LIQTRHRIPETPLTEDQIIIFQVPIPEPLRFIEPRETETRTMHALEEYGVMQVKLYED
                     IARFGHIATTYAYPVKVNGRYVMDPSPIPKFDNPKMDMMPALQLFGAGREKRIYAVPP
                     FTRVESLDFDDHPFTVQQWDEPCAICGSTHSYLDEVVLDDAGNRMFVCSDTDYCRQQS
                     EAKNQ"
     gene            7609..8367
                     /gene="phnK"
                     /locus_tag="ACHOSC_RS14230"
                     /old_locus_tag="pgaptmp_002846"
     CDS             7609..8367
                     /gene="phnK"
                     /locus_tag="ACHOSC_RS14230"
                     /old_locus_tag="pgaptmp_002846"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018835 - carbon phosphorus lyase activity
                     [Evidence IEA];
                     GO_process: GO:0019634 - organic phosphonate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate C-P lyase system protein PhnK"
                     /protein_id="HGU4548750.1"
                     /translation="MNQPLLSVNNLTHLYAPGKGFSDVSFDLWPGEVLGIVGESGSGK
                     TTLLKSISARLTPQQGEIHYESRSLYAMSEADRRRLLRTEWGVVHQHPLDGLRRQVSA
                     GGNIGERLMATGARHYGDIRATAQKWLEEVEIPANRIDDLPTTFSGGMQQRLQIARNL
                     VTHPKLVFMDEPTGGLDVSVQARLLDLLRGLVVELNLAVVIVTHDLGVARLLADRLLV
                     MKQGQVVESGLTDRVLDDPHHPYTQLLVSSVLQN"
     gene            8478..9158
                     /gene="phnL"
                     /locus_tag="ACHOSC_RS14235"
                     /old_locus_tag="pgaptmp_002847"
     CDS             8478..9158
                     /gene="phnL"
                     /locus_tag="ACHOSC_RS14235"
                     /old_locus_tag="pgaptmp_002847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018835 - carbon phosphorus lyase activity
                     [Evidence IEA];
                     GO_process: GO:0019634 - organic phosphonate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate C-P lyase system protein PhnL"
                     /protein_id="HGU4548751.1"
                     /translation="MINVQNVSKTFILHQQNGVRLPVLQRASLTVNAGECVVLHGHSG
                     SGKSTLLRSLYANYLPDEGQIQIKHGDEWVDLVTAPARKVVEIRKTTVGWVSQFLRVI
                     PRISALEVVMQPLLDTGVPREACAAKAARLLTRLNVPERLWHLAPSTFSGGEQQRVNI
                     ARGFIVDYPILLLDEPTASLDAKNSAAVVELIREAKTRGAAIVGIFHDEAVRNDVADR
                     LHPMGASA"
     gene            9155..10291
                     /gene="phnM"
                     /locus_tag="ACHOSC_RS14240"
                     /old_locus_tag="pgaptmp_002848"
     CDS             9155..10291
                     /gene="phnM"
                     /locus_tag="ACHOSC_RS14240"
                     /old_locus_tag="pgaptmp_002848"
                     /EC_number="3.6.1.63"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_process: GO:0019700 - organic phosphonate catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-D-ribose 1-methylphosphonate
                     5-triphosphate diphosphatase"
                     /protein_id="HGU4548752.1"
                     /translation="MIINNVKLVLENEVVHGSLEVQDGEIRAFAESQSRLPEAMDGEG
                     GWLLPGLIELHTDNLDKFFTPRPKVDWPAHSAMSSHDALMVASGITTVLDAVAIGDVR
                     DGGDRLENLEKMINAIEETQKRGVNRAEHRLHLRCELPHHTTLPLFEKLVQREPVTLV
                     SLMDHSPGQRQFANREKYREYYQGKYSLTDAQMQQYEEEQLALAARWSQPNRESIAAL
                     CHARQIALASHDDATHAHVAESHQLGSVIAEFPTTFEAAEASRKHGMNVLMGAPNIVR
                     GGSHSGNVAASELAQLGLLDILSSDYYPASLLDAAFRVADDESNRFTLPQAVRLVTKN
                     PAQALNLQDRGGIGEGKRADLVLAHRQGNHIHIDHVWRQGKRVF"
     gene            10291..10848
                     /gene="phnN"
                     /locus_tag="ACHOSC_RS14245"
                     /old_locus_tag="pgaptmp_002849"
     CDS             10291..10848
                     /gene="phnN"
                     /locus_tag="ACHOSC_RS14245"
                     /old_locus_tag="pgaptmp_002849"
                     /EC_number="2.7.4.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033863 - ribose 1,5-bisphosphate
                     phosphokinase activity [Evidence IEA];
                     GO_process: GO:0006015 - 5-phosphoribose 1-diphosphate
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose 1,5-bisphosphokinase"
                     /protein_id="HGU4548753.1"
                     /translation="MMGKLIWLMGPSGSGKDSLLAELRLREQTQLLVAHRYITRDASA
                     GSENHIALSEREFFTRAGQNLLALSWHANGLYYGVGIEIDLWLHAGFDVVVNGSRAHL
                     PQARARYQSALLPVCLQVSPEILRQRLENRGRENASEINARLARAARYTPQDCHTLNN
                     DGSLRQSVDTLLTLIHQKEKHHACL"
     gene            10835..11269
                     /gene="phnO"
                     /locus_tag="ACHOSC_RS14250"
                     /old_locus_tag="pgaptmp_002850"
     CDS             10835..11269
                     /gene="phnO"
                     /locus_tag="ACHOSC_RS14250"
                     /old_locus_tag="pgaptmp_002850"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoalkylphosphonate N-acetyltransferase"
                     /protein_id="HGU4548754.1"
                     /translation="MPACELRPATQYDTDAVYALICELKQAEFDHHAFRVGFNANLRD
                     PNMRYHLALLDGEVVGMIGLHLQFHLHHVNWIGEIQELVVMPQARGLNVGSKLLAWAE
                     EEARQAGAEMTELSTNVKRHDAHRFYLREGYEQSHFRFTKAL"
     gene            11271..12029
                     /gene="phnP"
                     /locus_tag="ACHOSC_RS14255"
                     /old_locus_tag="pgaptmp_002851"
     CDS             11271..12029
                     /gene="phnP"
                     /locus_tag="ACHOSC_RS14255"
                     /old_locus_tag="pgaptmp_002851"
                     /EC_number="3.1.4.55"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008081 - phosphoric diester hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0019700 - organic phosphonate catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate metabolism protein PhnP"
                     /protein_id="HGU4548755.1"
                     /translation="MSLTLTLTGTGGAQGVPAWGCECAACARARRSPQYRRQPCSGVV
                     KFNDAITLIDAGRHDLTDRWSPGSFQQFLLTHYHMDHVQGLFPLRWGVGDVIPVYGPP
                     DEQGCDDLFKHPGLLDFSHTVEPFVVFDLQGLQVTPLPLNHSKLTFGYLLETAHSRVA
                     WLSDTAGLPEKTLKFLLNNHPQVMVIDCSHPPRADAPRNHCDLNTVLALNQVIRSPQV
                     ILTHISHQFDAWLMENALPSGFEVGFDGMEIGVA"
     gene            complement(12176..12505)
                     /gene="yjdP"
                     /locus_tag="ACHOSC_RS14260"
                     /old_locus_tag="pgaptmp_002852"
     CDS             complement(12176..12505)
                     /gene="yjdP"
                     /locus_tag="ACHOSC_RS14260"
                     /old_locus_tag="pgaptmp_002852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DDRRRQL repeat protein YjdP"
                     /protein_id="HGU4548756.1"
                     /translation="MKRFPLFLLFTLLTLSTVPAQADIIDDTIGNIQQAINDAYNPDR
                     GRDYEDSRDDGWQREVSDDRRRQYDDRRRQFEDRRRQLDDRQRQLDQERRQLEDEERR
                     MEDEYGR"
     gene            complement(12574..12891)
                     /locus_tag="ACHOSC_RS14265"
                     /old_locus_tag="pgaptmp_002853"
     CDS             complement(12574..12891)
                     /locus_tag="ACHOSC_RS14265"
                     /old_locus_tag="pgaptmp_002853"
                     /EC_number="5.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001183281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RpiB/LacA/LacB family sugar-phosphate isomerase"
                     /protein_id="HGU4548757.1"
                     /translation="MQPVFLFKKRTGAMGGHVAGTAGFCVAVPAFYSWSMSEVIDKDT
                     RSSERTDYPHYASEVALAFGSRVVGLELAKMIVDAWLGAQYEGGRHQQRVEAITAIEQ
                     RRN"
BASE COUNT         2811 a         3912 c         3643 g         2548 t
ORIGIN      
        1 cgtttccttt caaatgaaaa cgatcgtcgt ctaaaatcag cagtaccccc gacaaactca
       61 gggattttgt gtataattgc ggcctttttc ggcaatctgc cgttttttgg cgcttttgcc
      121 ctgctgactt ttgaggaaat ccacatgtca ttaccacact gcccaaaatg caactccgaa
      181 tacacttacg aagataacgg catgtacatc tgcccggaat gtgcctacga atggaacgac
      241 gcagaacctg cacaggaaag cgacgagctg atcgttaaag atgctaacgg caatctgctg
      301 gctgacggcg acagcgttac catcattaaa gatctgaagg tgaaaggtag ctcttcgatg
      361 ctgaaaattg gcaccaaagt gaaaaacatc cgcctggttg aaggcgacca taacatcgat
      421 tgcaaaatcg acggttttgg tccgatgaaa ctgaaatctg agtttgtgaa aaagaactga
      481 ttgtattgtg atcggtaagc cggataaggc gctcgcgccg catccggcaa cgatgccgac
      541 tgcctgatgc gacgctgacc gcgtcttatc aggcttccct ctcatctgta taaatttcga
      601 actacactta actggcttct cttaactgag gtcaccatca tgtcgttaag tccctacctc
      661 tcttttgccg gtaactgtgc cgacgcaatt gcttattatc aacgtacgtt gggcgctgaa
      721 ctgctctata aaatcagctt cggcgaaatg ccaaaatcag cgcaggacag cgccgagaac
      781 tgcccttccg gaatgcaatt tcccgatacc gccatcgccc acgccaacgt gcgcattgcc
      841 ggaagcgaca tcatgatgag cgatgctata ccgtcaggaa aagccagcta ctccggcttt
      901 acgctggtgc tcgattcgca acaggtcgaa gaaggaaaac gctggtttga caatcttgcc
      961 gctaacggaa aaatcgaaat ggcctggcag gaaactttct gggcgcatgg ctttggcaaa
     1021 gtcaccgata aatttggcgt accgtggatg attaatgtcg tcaaacaaca accaacgcaa
     1081 taacccgccg ggaggcccgc cctcccgcac tgtcatcgaa ttcccgttaa ctcttcatct
     1141 gttagtcact tttaattaac caaatcgtca caataatccg ccacgatgga gccacttttt
     1201 tagggaggct gcatcatgca aacgattatc cgtgtcgaga agctcgccaa aaccttcaat
     1261 cagcatcagg cgctgcatgc ggttgatctg aacattcatc acggtgaaat ggtggctctg
     1321 cttgggccgt cgggttccgg caaatccacc cttttacgtc acttaagcgg tttgattacc
     1381 ggcgataaat ccgccggcag ccatatcgag ctgctgggcc gcacagtcca gcgcgaaggc
     1441 cgtctggcgc gcgatatccg caaaagccgc gccaacaccg gctacatctt ccaacaattc
     1501 aacctggtga accgcctgag cgtactggag aacgtgctga ttggcgcgct cggcagcacg
     1561 ccgttctggc gcacctgttt tagctggttc accggcgagc agaaacagcg cgcgttacag
     1621 gcgctgaccc gcgttggcat ggtgcatttt gcccatcagc gcgtttccac cctctccggc
     1681 ggacagcagc agcgtgtggc gattgcccgc gcgctgatgc agcaggcgaa ggtgattctg
     1741 gccgatgaac ccatcgcctc gctggacccg gaatccgccc gcatcgtgat ggacaccctg
     1801 cgcgacatca atcagaacga cggcatcacc gtggtcgtca cgctgcatca ggtggattac
     1861 gccctgcgct actgcgaacg catcgtcgcc ctgcgccagg ggcacgtttt ctacgacggc
     1921 agcagccaac agtttgataa cgaacgtttt gaccatctct accgcagcat taatcgcatc
     1981 gaagagaacg cgaaagctgc ctgacatccc catcattgag gaaaacgaat gaacgctaag
     2041 ataattgcct cgctggcctt caccagcatg ttcagcctca gcaccctgtt aagcccggca
     2101 cacgccgaag agcaggaaaa ggcgctgaat ttcggcatta tttcaacgga atcacagcaa
     2161 aacctgaaac cgcaatggac gccattctta caggatatgg agaagaagct gggcgtgaag
     2221 gtgaacgcct tctttgcccc agactacgca ggcattatcc agggaatgcg cttcaataaa
     2281 gtggatatcg cctggtacgg caacctgtcg gcaatggaag cggtggatcg cgccaacggc
     2341 caggtcttcg cccagacggt cgcggcggat ggatcgccag gttactggag cgtgttgatc
     2401 gtcaacaaag atagtccgat caacaacctg aacgatctgc tggcgaagcg gaaagatctc
     2461 accttcggca atggcgatcc taactccacc tctggcttcc tcgtccccgg ttactacgtc
     2521 tttgccaaaa acaatatctc cgccagcgac tttaagcgca ccgtcaacgc tgggcatgaa
     2581 accaacgcgc tggccgtcgc caacaagcag gtggatgttg ccaccaacaa caccgaaaac
     2641 ctcgacaagc tgaaaacctc cgcgccagag aagctgaaag aactgaaggt gatctggaag
     2701 tcgccgctga tcccaggcga tccgatcgtc tggcgtaaaa acctttccga aaccaccaaa
     2761 gacaagatct acgacttctt tatgaactac ggcaaaacgc cggaagaaaa agcggtgctg
     2821 gaacgcctgg gctgggcgcc gttccgcgct tccagcgacc tgcaactggt gccgattcgc
     2881 cagctcgcgc tgtttaaaga gatgcagggc gtgaaaagca ataaaggact gaatgagcag
     2941 gacaagctgg caaaaaccac cgagattcag gcgcagctgg atgacctgga ccgcctgaac
     3001 aacgcgttaa gcgcgatgag ttcggtgagt aaagcggtgc agtaaatcgt aggtcggata
     3061 agacgccccg gcgtcgcatc cgacaatgtg caggcgttga tgccggatgc ggtgcaagca
     3121 ccttatccgg cctacagacc ggagccaaac atgcaaacca tcaccatcgc cccacccaag
     3181 cgcagctggt tctcgcttct gagctgggcc gttgttctcg ccgtgttggt cgtctcgtgg
     3241 cagggcgcgg aaatggcccc gctcacgctg attaaagacg gcggcaacat ggcgacgttc
     3301 gccgccgact tcttcccgcc cgatttcagc cagtggcagg attacctcac cgaaatggcc
     3361 gtcacgctgc aaatcgccgt ctggggcacg gcgctggcgg tggttctctc catccccttc
     3421 ggcctgatga gcgccgaaaa cctggtgccg tggtgggttt accagcccgt tcgccgcctg
     3481 atggacgcct gccgcgtcat taacgaaatg gtcttcgcca tgctgttcgt ggtcgccgtc
     3541 ggcctcggcc cgttcgctgg cgtgctggcg ctgtttatcc acaccaccgg cgtgctctcc
     3601 aaactgcttt ccgaagcggt ggaagcgatt gaacccggcc cggtggaagg cattcgcgcc
     3661 accggcgcca acaagctcga agagatcctc tacggcgtgc tgccgcaggt gatgccgctg
     3721 ctgatctcct actccctcta tcgcttcgaa tccaacgtcc gctcggcgac cgtcgtcggc
     3781 atggtcggtg caggcgggat cggcgtcacc ctgtgggaag cgattcgcgg tttccagttc
     3841 caacaaacct gcgccctgat ggtgcttatc atcgtcacgg tcagcctgct ggatttcctc
     3901 tctcaacggt tgcgtaagca ctttatctga taagcgaggc attgatatct atgcacttgt
     3961 ctacacatcc gaccagctac ccaacacgct atcaggagat agccgcaaaa cttgagcagg
     4021 agcttcgtca acactaccgc tgcggcgact atcttcccgc cgagcagcaa ctggcggcgc
     4081 gctttgaggt gaaccgccac accctgcgcc gcgccatcga ccaactggtg gaaaaaggct
     4141 gggtacagcg ccgtcagggc gtcggcgtgc tggtgctgat gcgcccgttc gattacccgc
     4201 tcaacgccca ggcgcgtttt agccagaacc tgctggacca gggtagccat cccaccagcg
     4261 aaaaactgct gtcggtattg cgccccgcgt ccggtcacgt cgctgacgcg ctggggatta
     4321 ccgagggcga gaacgttatc catttgcgca ccctgcgtcg cgtcaacggc gtcgcgctct
     4381 gtttaatcga ccactacttt gcggacctca ccctttggcc gacgctgcaa cgcttcgaca
     4441 gcggttcgct gcacgatttt ctgcgcgagc aaaccggaat tgcgctgcgc cgcagccaga
     4501 cgcggatcag cgcccgtcgc gcccaggcca aagagtgcca gcgtcttgaa attccgaata
     4561 tgtcgccgct gctgtgcgtg cgcaccctta accaccgtga cggtgaaagc agcccggcgg
     4621 agtactccgt cagcctgacg cgcgccgaca tgattgaatt cactatggag cactgaatgc
     4681 acgcagatac cgcgacccgc cagcactgga tgtccgtact ggcgcacagc caaccggctg
     4741 aactggcagc ccgcctgaaa gcactgaaca tcaccgccga ctatgaggtt atccgcgccg
     4801 ccgaaaccgg cctggtgcag attcaggcgc ggatgggcgg caccggcgaa cgcttttttg
     4861 ccggcgacgc cacgctcacc cgcgccgccg tacgcctgac tgacggcacg ctcggctaca
     4921 gctgggtgct ggggcgcgac aaacagcacg ccgaacgctg cgcgctgatt gacgcgctga
     4981 tgcagcaatc tcgctatttt caaaacttat cagaaaccct tattgccccg ctggacgctg
     5041 accgtatggc acgcattgcc gcacgccagg ccgaagtgaa cgccagccgg gtcgacttct
     5101 ttacgatggt tcgcggagac aacgcatgac cctggaaacc gcttttatgc ttcccgtgca
     5161 ggatgcccag cacagttttc gtcgcctgtt aaaggccatg agcgagccgg gcgtgattgt
     5221 cgccctgcat cagctcaagc gcggctggca gccgctgaat atcgccacca ccagcgtgct
     5281 gctgacgctg gccgataacg acacgccggt gtggcttgcc gcgccattga gtaacgatat
     5341 cgtcagccag agcctgcgtt ttcataccaa cgcgccgctg gtcagccagc cggaacaggc
     5401 gaccttcgcg gtgacggatg aggcgatttc cagcgaacag cttaacgccc tttccaccgg
     5461 caccgccgtt gcgccggaag cgggtgcaac gctgatttta caggtcgcca gcctgagcgg
     5521 cgggcgcatg ttacgtctca ccggcgcggg cattgccgaa gaacgaatga tcgctccgca
     5581 gctgccggag tgcattctgc acgaactcac cgagcgcccg cacccgttcc cgctcggcat
     5641 cgacctgatc ctgacctgcg gcgagcgcct gctggctatt ccgcgaacca ctcatgtgga
     5701 ggtgtgctga tgtacgttgc cgtgaaaggg ggcgagaagg cgatcgacgc cgctcacgcc
     5761 ctgcaagaga gccgacgccg gggcgatacc gatttgcctg aactgagcgt cgcccagatt
     5821 gaacagcagc ttaatcttgc ggtggatcgc gtgatgaccg aaggcggcat tgccgaccgc
     5881 gaactggcgg cgctggcgct gaaacaggcc agcggcgata acgtcgaagc aattttcctg
     5941 ctgcgcgcct accgcaccac gctggcgaag ctcgcggtaa gcgagccgct cgacaccaca
     6001 gagatgcgtc tcgaacggcg tatttcggcg gtttataaag acattcccgg cggccagctg
     6061 cttggcccaa cctacgacta cacccatcgc ctgctcgatt tcaccctgct ggcaaacggt
     6121 gaagcgccga cgctgaccac cgccgacagc gaacaacagc cgtcgccgca cgttttcagc
     6181 ctgctggcgc gtcaggggct ggtgaagttt gaagaagata gcggcgcaca gccggatgac
     6241 atcacccgca cgccgccggt ttacccctgc tcacgctcct cccgcctgca acaattaatg
     6301 cgcggggacg aaggctattt gctggcgctg gcctactcca cccagcgtgg ttacgggcgc
     6361 aatcacccgt tcgcgggcga gatccgcagt ggttacatcg acgtgtcgat tgtgccggaa
     6421 gagctgggat ttgcggtaaa cgtcggcgaa ctactgatga ccgagtgtga aatggtcaac
     6481 ggttttatcg acccgccgga tgagccgccg cacttcacgc gcggctacgg gctggtattc
     6541 ggcatgagcg agcgcaaagc gatggcaatg gcgctggtcg atcgtgcgtt gcaggctccg
     6601 gaatacggcg agcacgcgac aggcccggcg caggatgaag agtttgtgct ggcacatgcc
     6661 gacaacgtcg aagccgcagg ctttgtctcg cacctcaaac tcccccacta cgtcgatttc
     6721 caggccgaac tggagctact caaacgtctg caacaggaga agaaccatgg ctaatctgag
     6781 cggctacaac tttgcctacc tcgacgagca gaccaaacgc atgatccgcc gcgctatctt
     6841 aaaagcggtg gcgatccccg gttatcaggt gccgtttggc gggcgcgaga tgccgatgcc
     6901 atacggctgg ggaaccggcg gcatacagct caccgccagc gtgattggcg aaagcgacgt
     6961 gctgaaggtg attgaccagg gtgcggatga caccaccaac gccgtgtcga ttcgcaactt
     7021 ctttaagcgc gtgaccgggg taaacaccac tgaacgtacg gacgatgcga cgcttatcca
     7081 gacgcgtcac cgcatccccg aaacgccgct gaccgaagat cagatcatta tcttccaggt
     7141 gccaatcccg gaaccgctgc gctttatcga gccgcgcgaa acggaaaccc gcaccatgca
     7201 cgcgctggaa gagtacggcg tgatgcaggt gaaactgtat gaagatatcg cccgcttcgg
     7261 tcatatcgcc actacctatg cctatccggt gaaggtgaac gggcgctacg taatggaccc
     7321 gtcgccgatc ccgaaattcg ataacccaaa aatggacatg atgcccgccc tgcaactgtt
     7381 cggcgcgggg cgcgagaagc gcatctatgc ggtgccgccg tttacccgcg tggaaagtct
     7441 cgatttcgac gatcacccgt tcaccgttca gcagtgggat gagccatgcg ccatctgcgg
     7501 atcgacccac agctatcttg atgaagtggt gctggatgac gccggaaacc gcatgtttgt
     7561 ctgctccgat accgattatt gccgccaaca gagcgaggca aaaaaccaat gaatcaaccg
     7621 ttactttcgg tcaataacct gacccacctt tacgcgccgg gcaaaggctt tagcgatgtc
     7681 tcttttgatt tatggccggg ggaagtgctg ggcattgtcg gggagtccgg ctccgggaag
     7741 accacgctgc tgaaatcaat ctccgcgcgc ctgacgccgc agcaggggga gattcactat
     7801 gagagccgtt cgctgtacgc aatgagcgag gccgaccgcc gtcgcctgct gcgcaccgaa
     7861 tggggcgtag tgcatcagca tccactcgac ggcctgcgcc gtcaggtctc ggcaggcggc
     7921 aatatcggcg agcggctgat ggcgaccggg gcacgtcatt acggcgatat tcgtgccacc
     7981 gcgcagaagt ggctggaaga ggtggaaatt cccgccaacc ggatcgacga cctgccgacc
     8041 accttttccg gcggcatgca gcaacgtttg cagatcgccc gcaacctggt gacgcatccg
     8101 aagctggtgt ttatggacga gccgaccggc ggactggatg tgtcggtaca ggcacgcctg
     8161 ctcgacctgc tgcgcggcct ggtggtggag ctgaacctcg cggtagtgat tgtcacccac
     8221 gatttaggcg tcgcccgcct gctggcggac cgtttgctgg tgatgaagca ggggcaagtg
     8281 gtggagagtg ggttaaccga ccgcgtgctc gacgacccgc accatccgta tacacaactg
     8341 ctggtgtcat cggttttgca gaattgagcc ggtgccggat gcggcgtaaa cgccttatcc
     8401 ggcctacaaa tgcgctcccc gtaggtcgga taagacgcgt cagcgtcgca tccgacaccc
     8461 gaaccacgag gcgaaaaatg attaacgtac aaaacgtcag taaaaccttc atcctgcacc
     8521 agcaaaacgg cgtgcgcctg cccgtcttac aacgtgcctc gctcaccgtc aacgcgggcg
     8581 aatgcgtggt gctccacggc cattccggca gcggcaaatc aaccttgctg cgctcgctgt
     8641 acgccaacta tctgcccgac gaagggcaaa tccagatcaa acacggtgac gaatgggtcg
     8701 atctggtcac cgcgccagcg cgcaaagtgg tggagatccg caaaaccacc gtcggctggg
     8761 tgagccagtt tctgcgcgtc atcccgcgta tttcagcact ggaagtggtg atgcagccgc
     8821 tgctcgatac cggcgttccg cgtgaagcct gcgccgctaa agccgcgcgt ctgctcaccc
     8881 gcctgaacgt gccggaacgc ctgtggcacc tggcgccatc aaccttttcc ggtggcgaac
     8941 agcagcgcgt caacatcgcc cgcggcttta tcgtcgacta ccccattctg ctgctcgacg
     9001 aacctaccgc ctcgctggac gccaaaaaca gcgccgccgt agtcgagctg attcgcgaag
     9061 ccaaaacccg tggcgcggcc atcgtcggca tcttccatga cgaagccgta cgtaatgacg
     9121 tcgccgaccg cttgcaccca atgggagcct ctgcatgatt atcaataacg tcaaactggt
     9181 gctggaaaac gaagtggtac acggttcgct ggaggtgcag gatggcgaaa tccgcgcctt
     9241 tgccgaaagc cagagccgcc tgccagaagc gatggacggc gaaggtggct ggctactacc
     9301 cggcctgatt gagctgcata ccgacaacct cgataaattc ttcaccccgc gcccgaaagt
     9361 cgactggccc gcccattcgg cgatgagcag ccacgacgcg ctgatggtgg caagcggcat
     9421 caccaccgta ctggacgccg tggcgattgg cgacgtgcgc gacggcggcg atcggctgga
     9481 gaatctggag aagatgatca acgccatcga agagacgcaa aaacgcggcg tcaaccgcgc
     9541 cgagcaccgc ctgcacctgc gctgcgaact gccgcatcac accacactgc cgctgtttga
     9601 aaaactggtt cagcgcgaac cggtgacgct ggtgtcgctg atggaccact caccgggcca
     9661 gcgccagttc gctaaccgcg agaagtatcg cgaatattat cagggcaaat actccctcac
     9721 cgatgcgcag atgcagcagt acgaagaaga gcaattggcg ctcgccgcac gctggtcgca
     9781 gccgaatcgc gaatccatcg ccgccctgtg ccacgcgcga caaattgcgc tcgccagcca
     9841 cgatgacgcc acgcacgccc acgttgccga atcccaccaa cttggcagcg tgatcgccga
     9901 atttcccacc acgttcgaag cggcggaagc ctcgcgtaag catggcatga acgtgctgat
     9961 gggtgcgcca aatatcgtgc gtggcggctc gcactccggc aatgtggcgg ccagtgaact
    10021 ggcgcagctt ggtctgctgg atatcctctc ttccgactac taccccgcca gcctgctgga
    10081 tgcggcgttc cgcgtcgccg atgacgagag caaccgcttt acgctaccgc aggcggtgag
    10141 gctggtgacc aaaaatccgg cgcaggcgct gaatcttcag gatcgcgggg ggattggcga
    10201 gggtaaacgc gctgacctgg tgctggcgca tcgccagggc aatcacattc atatcgacca
    10261 cgtctggcgt cagggtaaaa gggtgttctg atgatgggaa aactgatttg gttaatgggg
    10321 ccgtccggct ccgggaaaga cagcctgctg gcggaactcc gcctgcggga acaaacgcag
    10381 ttactggtgg cgcatcgcta catcacgcgc gacgccagcg ccggaagtga aaaccatatc
    10441 gccctgagtg agcgggagtt ttttacccgc gcggggcaaa acctgctggc cttaagctgg
    10501 cacgctaacg gtctgtatta tggcgtcggc atcgagattg acctctggct gcacgctgga
    10561 ttcgacgtag tcgtcaacgg ttcacgcgcg catttgccgc aggcacgggc gcgctatcaa
    10621 tcggcgctgc tgcccgtctg tttacaggtt tcgccggaga tcctgcgcca gcgcctggaa
    10681 aaccgtggcc gtgaaaacgc cagtgaaatt aacgcccggc tggcgcgcgc cgcccgctat
    10741 actccacagg attgccatac gctcaacaat gacggcagcc tgcgccagtc ggtcgacaca
    10801 ctgctgacgc tgattcatca gaaggagaaa caccatgcct gcttgtgagc ttcgcccggc
    10861 cacgcagtac gataccgacg cggtttacgc gctgatttgt gagctaaaac aggcggagtt
    10921 tgaccaccac gcgtttcgcg tgggctttaa cgccaacctg cgcgacccga acatgcgcta
    10981 ccatctggcg ctgctggatg gcgaagttgt cggcatgatc ggcctgcatt tgcagtttca
    11041 tctgcatcat gtcaactgga tcggcgaaat tcaggaactg gtggtgatgc cgcaggcgcg
    11101 cggcctgaac gtcggtagca agttgctggc gtgggcagaa gaagaagccc gccaggccgg
    11161 ggccgaaatg accgagcttt cgaccaacgt gaagcgccac gacgcgcacc gtttctatct
    11221 gcgagaaggc tacgagcaga gccacttccg cttcaccaag gcgctgtaac atgagcctga
    11281 ccctcacgct caccggcact ggcggcgcac agggcgttcc ggcatggggc tgcgagtgcg
    11341 cggcctgcgc cagagcgcgg cgctcgccgc agtatcgccg ccagccatgc agcggcgtag
    11401 tgaagtttaa cgacgcgatc accctgatcg acgccgggcg gcacgatctc accgatcgct
    11461 ggtcgcctgg atcgttccag cagtttttgc tgacgcacta ccatatggat cacgtccagg
    11521 ggctatttcc gctacgctgg ggcgtgggcg atgtgatccc ggtctacggc ccgccggatg
    11581 aacagggctg cgacgatctg tttaaacatc cgggcctgct tgatttcagc cacacagtgg
    11641 aaccgtttgt ggtgtttgat ttgcaggggt tgcaggtcac gccgctgccg cttaaccact
    11701 caaaactgac cttcggttat ctgctggaaa cggcacatag ccgggtggcg tggctgtctg
    11761 acaccgctgg cctgccggaa aaaacgctga aatttttact caataatcat ccgcaggtga
    11821 tggtgattga ttgcagtcac ccgccgcgcg cggatgcacc gcgtaatcac tgtgatttaa
    11881 ataccgtgct tgcgctgaat caggttatcc gctcgccaca ggtgattctg acccatatca
    11941 gccaccagtt tgatgcgtgg ctgatggaaa acgcactacc gtcagggttt gaggtggggt
    12001 ttgatgggat ggagattggg gtggcgtgat gagagggaat gtgcgcgctg gccccctcac
    12061 cctaaccctc tccccagagg ggcgagggga ccgattgtgc tcaatattga atattgcgct
    12121 cgttttctcc ctctccccat tggggtgagg ggcgatgcct gctccatacc caacctcatc
    12181 gcccatactc atcttccatt ctccgctctt catcctccag ttgccgacgc tcctgatcaa
    12241 gctggcgctg gcgatcgtcc agctgcctgc ggcgatcttc aaactggcgg cggcggtcgt
    12301 catattgtct gcgccgatcg tcgctcactt cacgctgcca gccgtcgtcg cgcgaatctt
    12361 catagtctcg cccacggtca gggttataag cgtcattaat cgcctgctga atattaccaa
    12421 tagtatcgtc gataatatca gcctgggccg gaacggtgga cagcgtgagc agggtgaata
    12481 aaagaaatag cggaaagcgt ttcattagcc aacctcaaaa agaaactcta tccacattag
    12541 tcattactca tccatgcaag tagtggatga atctcaattt ctccgctgct ctattgccgt
    12601 aatcgcctcc acgcgttgtt gatgacgacc gccttcgtac tgtgcgccca gccacgcatc
    12661 cacaatcatt tttgccagtt cgaggccaac cactcgtgaa ccaaaagcca gtgcaacttc
    12721 actggcataa tgcggataat cagtgcgctc tgacgacctg gtgtctttat caatcacctc
    12781 actcattgac catgaataaa aggctggcac agcaacacag aatcctgcgg tgccagcgac
    12841 atgaccgccc attgctccgg tacgtttttt aaaaagaaac accggttgca ttgataaatt
    12901 gtgctcagta tttg
//
LOCUS       DAZKHF020000124        12884 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2201S31241
            SAMN43267800-rid25106413.denovo.125, whole genome shotgun sequence.
ACCESSION   DAZKHF020000124 DAZKHF020000000
VERSION     DAZKHF020000124.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267800
            Sequence Read Archive: SRR30307263
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 12884)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12884)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 12884)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350581.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350581.2
            Genome Coverage       :: 151x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:21:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,652
            CDSs (total)                      :: 5,557
            Genes (coding)                    :: 5,320
            CDSs (with protein)               :: 5,320
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 237
            CDSs (without protein)            :: 237
            Pseudo Genes (ambiguous residues) :: 0 of 237
            Pseudo Genes (frameshifted)       :: 102 of 237
            Pseudo Genes (incomplete)         :: 130 of 237
            Pseudo Genes (internal stop)      :: 52 of 237
            Pseudo Genes (multiple problems)  :: 41 of 237
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12884
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267800-rid25106413.denovo.125"
                     /serotype="O 26:H11"
                     /isolate="2201S31241"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-01"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            233..814
                     /gene="mdaB"
                     /locus_tag="ACHOSC_RS14270"
                     /old_locus_tag="pgaptmp_002854"
     CDS             233..814
                     /gene="mdaB"
                     /locus_tag="ACHOSC_RS14270"
                     /old_locus_tag="pgaptmp_002854"
                     /EC_number="1.6.5.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH:quinone oxidoreductase MdaB"
                     /protein_id="HGU4548758.1"
                     /translation="MSNILIINGAKKFAHSNGQLNDTLTEVADGTLRDLGHDVRIVRA
                     DSDYDVKAEVQNFLWADVVIWQMPGWWMGAPWTVKKYIDDVFTEGHGTLYASDGRTRK
                     DPSKKYGSGGLVQGKKYMLSLTWNAPMEAFTEKDQFFHGVGVDGVYLPFHKANQFLGM
                     EPLPTFIANDVIKMPDVPRYTEEYRKHLVEIFG"
     gene            845..1159
                     /locus_tag="ACHOSC_RS14275"
                     /old_locus_tag="pgaptmp_002855"
     CDS             845..1159
                     /locus_tag="ACHOSC_RS14275"
                     /old_locus_tag="pgaptmp_002855"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004497 - monooxygenase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative quinol monooxygenase"
                     /protein_id="HGU4548759.1"
                     /translation="MLTVIAEIRTRPGQHHRQAVLDQFAKIVPTVLKEEGCHGYAPMV
                     DCAAGVSFQSMAPDSIVMIEQWESIAHLEAHLQTPHMKAYSEAVKGDVLEMNIRILQP
                     GI"
     gene            complement(1207..3099)
                     /gene="parE"
                     /locus_tag="ACHOSC_RS14280"
                     /old_locus_tag="pgaptmp_002856"
     CDS             complement(1207..3099)
                     /gene="parE"
                     /locus_tag="ACHOSC_RS14280"
                     /old_locus_tag="pgaptmp_002856"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005694 - chromosome [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IV subunit B"
                     /protein_id="HGU4548760.1"
                     /translation="MTQTYNADAIEVLTGLEPVRRRPGMYTDTTRPNHLGQEVIDNSV
                     DEALAGHAKRVDVILHADQSLEVIDDGRGMPVDIHPEEGVPAVELILCRLHAGGKFSN
                     KNYQFSGGLHGVGISVVNALSKRVEVNVRRDGQVYNIAFENGEKVQDLQVVGTCGKRN
                     TGTSVHFWPDETFFDSPRFSVSRLTHVLKAKAVLCPGVEITFKDEINNTEQRWCYQDG
                     LNDYLAEAVNGLPTLPEKPFIGNFAGDTEAVDWALLWLPEGGELLTESYVNLIPTMQG
                     GTHVNGLRQGLLDAMREFCEYRNILPRGVKLSAEDIWDRCAYVLSVKMQDPQFAGQTK
                     ERLSSRQCAAFVSGVVKDAFILWLNQNVQAAELLAEMAISSAQRRMRAAKKVVRKKLT
                     SGPALPGKLADCTAQDLNRTELFLVEGDSAGGSAKQARDREYQAIMPLKGKILNTWEV
                     SSDEVLASQEVHDISVAIGIDPDSDDLSQLRYGKICILADADSDGLHIATLLCALFVK
                     HFRALVKHGHVYVALPPLYRIDLGKEVYYALTEEEKEGVLEQLKRKKGKPNVQRFKGL
                     GEMNPMQLRETTLDPNTRRLVQLTIDDEDDQRTDAMMDMLLAKKRSEDRRNWLQEKGD
                     MAEIEV"
     gene            complement(3128..3709)
                     /gene="yqiA"
                     /locus_tag="ACHOSC_RS14285"
                     /old_locus_tag="pgaptmp_002857"
     CDS             complement(3128..3709)
                     /gene="yqiA"
                     /locus_tag="ACHOSC_RS14285"
                     /old_locus_tag="pgaptmp_002857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase YqiA"
                     /protein_id="HGU4548761.1"
                     /translation="MSTLLYLHGFNSSPRSAKASLLKNWLAEHHPDVEMIIPQLPPYP
                     SDAAELLESIVLEHGGDSLGIVGSSLGGYYATWLSQCFMLPAVVVNPAVRPFELLTDY
                     LGQNENPYTGQQYVLESRHIYDLKVMQIDPLEAPDLIWLLQQTGDEVLDYRQAVAYYA
                     SCRQTVIEGGNHAFTGFEDYFNPIVDFLGLHHL"
     gene            complement(3709..4536)
                     /gene="cpdA"
                     /locus_tag="ACHOSC_RS14290"
                     /old_locus_tag="pgaptmp_002858"
     CDS             complement(3709..4536)
                     /gene="cpdA"
                     /locus_tag="ACHOSC_RS14290"
                     /old_locus_tag="pgaptmp_002858"
                     /EC_number="3.1.4.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004115 - 3',5'-cyclic-AMP
                     phosphodiesterase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3',5'-cyclic-AMP phosphodiesterase"
                     /protein_id="HGU4548762.1"
                     /translation="MESLLTLPLAGEARVRILQITDTHLFAQKHEALLGVNTWESYQA
                     VLEAIRPHQHEFDLIVATGDLAQDQSSAAYRHFAEGIASFRAPCVWLPGNHDFQPAMY
                     SALQDAGISPAKRVFIGEQWQILLLDSQVFGVPHGELSEFQLEWLERKLADAPERHTL
                     LLLHHHPLPAGCSWLDQHSLRNAGELDTVLAKFPHVKYLLCGHIHQELDLDWNGRRLL
                     ATPSTCVQFKPHCSNFTLDTIAPGWRTLELHADGTLTTEVHRLADTRFQPDTASEGY"
     gene            complement(4561..4983)
                     /locus_tag="ACHOSC_RS14295"
                     /old_locus_tag="pgaptmp_002859"
     CDS             complement(4561..4983)
                     /locus_tag="ACHOSC_RS14295"
                     /old_locus_tag="pgaptmp_002859"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1249 family protein"
                     /protein_id="HGU4548763.1"
                     /translation="MKRYTPDFPEMMRLCEMNFSQLRRLLPRNDAPGETVSYQVANAQ
                     YRLTIVESTRYTTLVTIEQTAPAISYWSLPSMTVRLYHDAMVAEVCSSQQIFRFKARY
                     DYPNKKLHQRDEKHQINQFLADWLRYCLAHGAMAIPVY"
     gene            complement(4984..5613)
                     /gene="nudF"
                     /locus_tag="ACHOSC_RS14300"
                     /old_locus_tag="pgaptmp_002860"
     CDS             complement(4984..5613)
                     /gene="nudF"
                     /locus_tag="ACHOSC_RS14300"
                     /old_locus_tag="pgaptmp_002860"
                     /EC_number="3.6.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-ribose diphosphatase"
                     /protein_id="HGU4548764.1"
                     /translation="MLKPDNLPVTFGKNDVEIIARETLYRGFFSLDLYRFRHRLFNGQ
                     MSHEVRREIFERGHAAVLLPFDPVRDEVVLIEQIRIAAYDTSETPWLLEMVAGMIEEG
                     ESVEDVARREAIEEAGLIVKRTKPVLSFLASPGGTSERSSIMVGEVDATTASGIHGLA
                     DENEDIRVHVVSREQAYQWVEEGKIDNAASVIALQWLQLHHQALKNEWA"
     gene            5818..7299
                     /gene="tolC"
                     /locus_tag="ACHOSC_RS14305"
                     /old_locus_tag="pgaptmp_002861"
     CDS             5818..7299
                     /gene="tolC"
                     /locus_tag="ACHOSC_RS14305"
                     /old_locus_tag="pgaptmp_002861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417507.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane channel protein TolC"
                     /protein_id="HGU4548765.1"
                     /translation="MKKLLPILIGLSLSGFSSLSQAENLMQVYQQARLSNPELRKSAA
                     DRDAAFEKINEARSPLLPQLGLGADYTYSNGYRDANGINSNATSASLQLTQSIFDMSK
                     WRALTLQEKAAGIQDVTYQTDQQTLILNTATAYFNVLNAIDVLSYTQAQKEAIYRQLD
                     QTTQRFNVGLVAITDVQNARAQYDTVLANEVTARNNLDNAVEQLRQITGNYYPELAAL
                     NVENFKTDKPQPVNALLKEAEKRNLSLLQARLSQDLAREQIRQAQDGHLPTLDLTAST
                     GISDTSYSGSKTRGAAGTQYDDSNMGQNKVGLSFSLPIYQGGMVNSQVKQAQYNFVGA
                     SEQLESAHRSVVQTVRSSFNNINASISSINAYKQAVVSAQSSLDAMEAGYSVGTRTIV
                     DVLDATTTLYNAKQELANARYNYLINQLNIKSALGTLNEQDLLALNNALSKPVSTNPE
                     NVAPQTPEQNAIADGYAPDSPAPVVQQTSARTTTSNGHNPFRN"
     gene            7447..8118
                     /locus_tag="ACHOSC_RS14310"
                     /old_locus_tag="pgaptmp_002862"
     CDS             7447..8118
                     /locus_tag="ACHOSC_RS14310"
                     /old_locus_tag="pgaptmp_002862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417509.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1190 family protein"
                     /protein_id="HGU4548766.1"
                     /translation="MKRTKSIRHASFRKNWSARHLTPVALAVATVFMLAGCEKSDETV
                     SLYQNADDCSAANPGKSAECTTAYNNALKEAERTAPKYATREDCVAEFGEGQCQQAPA
                     QAGMAPENQAQAQQSSGSFWMPLMAGYMMGRLMGGGAGFAQQPLFSSKNPASPAYGKY
                     TDATGKNYGAAQPGRTMTVPKTAMAPKPATTTTVTRGGFGESVAKQSTMQRSATGTSS
                     RSMGG"
     gene            8124..9284
                     /locus_tag="ACHOSC_RS14315"
                     /old_locus_tag="pgaptmp_002863"
     CDS             8124..9284
                     /locus_tag="ACHOSC_RS14315"
                     /old_locus_tag="pgaptmp_002863"
                     /EC_number="6.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathionylspermidine synthase family protein"
                     /protein_id="HGU4548767.1"
                     /translation="MERVSITERPDWREKAHEYGFNFHTMYGEPYWCEDAYYKLTLAQ
                     VEKLEEVTAELHQMCLKVVEKVIASDELMTKFRIPKHTWSFVRQSWLTHQPSLYSRLD
                     LAWDGTGEPKLLENNADTPTSLYEAAFFQWIWLEDQLNAGNLPEGSDQFNSLQEKLID
                     RFVELREQYGFQLLHLTCCRDTVEDRGTIQYLQDCATEAEIATEFLYIDDIGLGEKGQ
                     FTDLQDQVISNLFKLYPWEFMLREMFSTKLEDAGVRWLEPAWKSIISNKALLPLLWEM
                     FPNHPNLLPAYFAEDDHPQMEKYVVKPIFSREGANVSIIENGKTIEAAEGPYGEEGMI
                     VQQFHPLPKFGDSYMLIGSWLVNDQPAGIGIREDRALITQDMSRFYPHIFVE"
     gene            complement(9322..10110)
                     /gene="ygiD"
                     /locus_tag="ACHOSC_RS14320"
                     /old_locus_tag="pgaptmp_002864"
     CDS             complement(9322..10110)
                     /gene="ygiD"
                     /locus_tag="ACHOSC_RS14320"
                     /old_locus_tag="pgaptmp_002864"
                     /EC_number="1.13.11.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001356237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4,5-DOPA dioxygenase extradiol"
                     /protein_id="HGU4548768.1"
                     /translation="MSSTRMPALFLGHGSPMNVLEDNLYTRSWQKLGMTLPRPQAIVV
                     VSAHWFTRGTGVTAMETPPTIHDFGGFPQALYDTHYPAPGSPVLAQHLVELLAPVPVT
                     LDKEAWGFDHGSWGVLIKMYPDADIPMVQLSIDSSKPAAWHFEMGRKLAALRDEGIML
                     VASGNVVHNLRTVKWHGDSSPYPWAMSFNEYVKANLTWQGAVEQHPLVNYLDHEGGAL
                     SNPTPEHYLPLLYVLGAWDGQEPITIPVDGIEMGSLSMLSVQIG"
     gene            10253..11026
                     /gene="zupT"
                     /locus_tag="ACHOSC_RS14325"
                     /old_locus_tag="pgaptmp_002865"
     CDS             10253..11026
                     /gene="zupT"
                     /locus_tag="ACHOSC_RS14325"
                     /old_locus_tag="pgaptmp_002865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc transporter ZupT"
                     /protein_id="HGU4548769.1"
                     /translation="MSVPLILTILAGAATFIGAFLGVLGQKPSNRLLAFSLGFAAGIM
                     LLISLMEMLPAALAAEGMSPVLGYGMFIFGLLGYFGLDRMLPHAHPQDLMQKSVQPLP
                     KSIKRTAILLTLGISLHNFPEGIATFVTASSNLELGFGIALAVALHNIPEGLAVAGPV
                     YAATGSKRTAILWAGISGLAEILGGVLAWLILGSMISPVVMAAIMAAVAGIMVALSVD
                     ELMPLAKEIDPNNNPSYGVLCGMSVMGFSLVLLQTAGIG"
     gene            complement(11084..11254)
                     /gene="yqiD"
                     /locus_tag="ACHOSC_RS14330"
                     /old_locus_tag="pgaptmp_002866"
     CDS             complement(11084..11254)
                     /gene="yqiD"
                     /locus_tag="ACHOSC_RS14330"
                     /old_locus_tag="pgaptmp_002866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YqiD"
                     /protein_id="HGU4548770.1"
                     /translation="MFIAWYWIVLIALVVVGYFLHLKRYCRAFRQDRDALLEARNKYL
                     NSTREETAEKVE"
     gene            complement(11515..12168)
                     /gene="ribB"
                     /locus_tag="ACHOSC_RS14335"
                     /old_locus_tag="pgaptmp_002867"
     CDS             complement(11515..12168)
                     /gene="ribB"
                     /locus_tag="ACHOSC_RS14335"
                     /old_locus_tag="pgaptmp_002867"
                     /EC_number="4.1.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008686 -
                     3,4-dihydroxy-2-butanone-4-phosphate synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3,4-dihydroxy-2-butanone-4-phosphate synthase"
                     /protein_id="HGU4548771.1"
                     /translation="MNQTLLSSFGTPFERVENALAALREGRGVMVLDDEDRENEGDMI
                     FPAETMTVEQMALTIRHGSGIVCLCITEDRRKQLDLPMMVENNTSAYGTGFTVTIEAA
                     EGVTTGVSAADRITTVRAAIADGAKPSDLNRPGHVFPLRAQAGGVLTRGGHTEATIDL
                     MTLAGFKPAGVLCELTNDDGTMARAPECIEFANKHNMALVTIEDLVAYRQAHERKAS"
     regulatory      complement(12272..12420)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00050"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="FMN riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="flavin mononucleotide"
                     /db_xref="RFAM:RF00050"
     gene            12542..12832
                     /gene="ubiK"
                     /locus_tag="ACHOSC_RS14340"
                     /old_locus_tag="pgaptmp_002868"
     CDS             12542..12832
                     /gene="ubiK"
                     /locus_tag="ACHOSC_RS14340"
                     /old_locus_tag="pgaptmp_002868"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417514.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ubiquinone biosynthesis accessory factor UbiK"
                     /protein_id="HGU4548772.1"
                     /translation="MIDPKKIEQIARQVHESMPKGIREFGEDVEKKIRQTLQAQLTRL
                     DLVSREEFDVQTQVLLRTREKLALLEQRMSELENRSTEIKKQPDPETLPPTL"
BASE COUNT         3252 a         3449 c         3163 g         3020 t
ORIGIN      
        1 tcaatcccaa aaaaaaccga accaaacgtg taaattattc cggtgcgagt aagaattatg
       61 aggaatggct atcagtattg tcattttcag aaaatattta tcctgcatcg gtgagtcaga
      121 gcaagatcag acttttgcta aattcgcaaa agactttgca cattttgcta atttcaccgt
      181 accgctctgt gacgtactat agtcggcaaa cgtctcacct tgaggttaaa aaatgagcaa
      241 catcctgatt atcaacggcg cgaaaaaatt cgcccactcc aatggtcaac tgaacgacac
      301 cctgaccgaa gtcgcggatg gcacactgcg cgacctcggg catgatgtcc gcatcgttcg
      361 cgccgacagc gactacgatg tcaaagcgga agtacaaaac tttctctggg ctgatgtggt
      421 gatctggcag atgccaggct ggtggatggg cgcgccgtgg acagtgaaaa aatatattga
      481 tgatgtattc accgaaggtc atggaacgct gtatgccagc gatggccgta cccgcaaaga
      541 tccgtcaaaa aaatacggtt ccggcggcct ggtacagggc aaaaaatata tgctttctct
      601 gacctggaac gcaccaatgg aagccttcac cgaaaaagat caattcttcc acggcgttgg
      661 cgtcgacggt gtgtatctgc cgttccataa agcaaaccaa ttccttggca tggaaccgct
      721 gccgacattt atcgctaatg acgtgataaa aatgcctgat gttccccgct atactgaaga
      781 atatcgcaag catcttgtgg aaatttttgg ttaactagag ctcaggcttt agaaggagtt
      841 aaccatgctt accgtaatcg cagaaatccg tactcgtcct ggtcaacatc accgtcaggc
      901 ggtattggat cagtttgcta aaatcgttcc aaccgtactg aaagaagaag gttgccacgg
      961 ctatgcgcca atggtggatt gcgctgctgg cgtgagtttc cagtctatgg caccggattc
     1021 tatcgtgatg attgagcagt gggaaagcat cgcgcatctt gaagcgcatc tgcaaacccc
     1081 gcacatgaag gcgtacagcg aagccgtaaa aggcgacgtg ctggagatga atatccgcat
     1141 cctgcaacct gggatttaaa cctgccttgt ttgcccggcc atcctgaccg ggcaatgttc
     1201 tttcctttaa acctcaatct ccgccatgtc gcctttctct tgcaaccagt tgcggcgatc
     1261 ttccgagcgt ttcttcgcca gcagcatatc catcatcgcg tcagtacgct gatcgtcttc
     1321 atcatcgata gtcaactgca ccagacggcg agtgttcgga tcaagcgtgg tttcacgcaa
     1381 ttgcatcggg ttcatttccc ccagaccttt aaaacgctgg acgttcggct tgcctttctt
     1441 gcgttttaat tgctcaagta cgccctcttt ctcttcttcc gtcagcgcgt aataaacctc
     1501 tttcccgaga tcaatacggt agagcggtgg cagtgcgacg taaacgtgac cgtgtttcac
     1561 caacgcgcgg aagtgtttta cgaacaaagc gcagagcagc gtggcaatgt gcagaccatc
     1621 agagtccgca tccgcgagga tacagatttt gccataacga agctggctca gatcgtcgct
     1681 gtcaggatcg ataccgatcg ctaccgaaat atcatgcact tcctgcgagg ccagcacttc
     1741 gtcggaagaa acttcccagg tgttaaggat cttacctttc agtggcatga tcgcctgata
     1801 ttcgcgatcg cgcgcctgct tggcagatcc gcctgcggag tcaccttcca caaggaacag
     1861 ttcggtacgg ttaagatcct gcgcggtaca gtcagccagt ttgccaggca gtgctgggcc
     1921 gctggtcagc tttttgcgca ccactttttt agccgcacgc atacggcgct gggcgctgga
     1981 aatcgccatc tccgccagca gctccgccgc ctgaacgttc tggttcagcc acaggataaa
     2041 ggcatctttc accacgcctg aaacgaatgc cgcgcattga cgcgaagaga gacgctcttt
     2101 ggtctgcccg gcaaactgcg gatcctgcat tttcaccgac agaacatagg cgcagcgatc
     2161 ccagatatct tccgccgaca gcttcacgcc gcgcggcaga atgttgcggt attcacagaa
     2221 ctcacgcatc gcgtccaaca ggccctgacg cagaccatta acatgggtac cgccctgcat
     2281 cgttgggata aggttgacgt agctttcggt cagcagttca ccgccttccg gcagccacag
     2341 tagcgcccag tccacagctt cagtatcgcc agcgaaatta ccgataaacg gtttttctgg
     2401 cagcgtcggt aaaccgttta ccgcttccgc caggtaatca ttcagaccgt cctgatagca
     2461 ccagcgctgt tcggtgttgt tgatctcatc tttaaaggta atctcaacgc cggggcacag
     2521 tactgctttg gccttcagca catgcgtcag gcgtgaaaca gaaaaacgcg ggctgtcaaa
     2581 gaaggtttca tccggccaga agtgcacgct ggtaccggta ttgcgtttac cgcaagtgcc
     2641 gacaacctgt aaatcctgca ccttttcgcc attttcaaag gcgatgttat aaacctgacc
     2701 atcgcggcgc acgttaactt ctacgcgctt cgacagggcg ttaaccaccg aaatccccac
     2761 gccatgcagg ccgccagaga actggtaatt tttgttagag aatttaccgc ctgcatgcag
     2821 acggcaaaga atcagttcaa ccgccggtac accctcttcc gggtgaatat ccaccggcat
     2881 cccgcgccca tcgtcaataa cttctaacga ctggtcagca tgtaagatta catccacgcg
     2941 ttttgcgtga cccgccagtg cttcatcgac actgttatca ataacttctt gccccaaatg
     3001 gttagggcga gtggtatcgg tatacatccc cggacggcgg cgaaccggct caagcccggt
     3061 gagtacctca atggcatcag cgttataagt ttgcgtcatg gtttaagtta gcaattcgag
     3121 ttgatcgtca gagatggtgc agaccaagaa aatcgacgat cgggttgaaa taatcttcga
     3181 agcccgtgaa tgcgtggttg ccgccttcta tgacagtctg gcggcaggaa gcgtagtacg
     3241 ccaccgcctg gcggtaatcc agcacttcat ctcccgtctg ttgcagcagc cagatcaaat
     3301 ccggcgcttc cagcgggtca atctgcatga ctttgagatc gtaaatatgg cgtgactcta
     3361 gcacatattg ctgcccggtg taggggttct cgttctgacc gagatagtcc gtcagcagtt
     3421 caaacgggcg caccgccggg tttaccacca ctgcgggcag cataaaacat tgtgacaacc
     3481 aggtggcgta atatcccccc agtgacgaac cgacaatacc cagcgaatcg ccgccatgtt
     3541 ccaggacaat ggattccagc agctctgccg cgtcggaagg atacggcggc aactgcggaa
     3601 tgatcatctc aacgtcaggg tgatgttccg ccagccagtt ttttaacaag ctcgcttttg
     3661 cagagcgcgg cgagctgttg aaaccgtgta aataaagaag cgtagacatc agtagccttc
     3721 tgaagcggta tcaggttgga aacgtgtgtc cgccaggcga tgcacctcgg tggtcagcgt
     3781 gccatcagca tgtaactcga gagtacgcca gccgggcgcg atggtgtcca gcgtaaagtt
     3841 ggaacagtgc ggcttaaact gcacacaggt cgacggcgtt gccagcaggc ggcgaccgtt
     3901 ccagtcgaga tccagctcct gatgaatatg accgcacagc aagtatttga cgtgcggaaa
     3961 cttcgccagc acggtatcca gttcgcccgc gttacgcagg ctgtgttgat caagccaact
     4021 acaacccgca ggtagcggat gatgatgcag cagcagcaac gtatggcgtt ctggcgcatc
     4081 ggccagttta cgttccagcc actcaagctg aaactcgctc agctcaccgt gcggcacgcc
     4141 aaacacctgg ctatccagca acaggatttg ccattgctca ccaataaaca cccgcttcgc
     4201 cggggagata cccgcatcct gtaacgcgct gtacatcgcg ggctggaaat cgtggttgcc
     4261 aggcagccag acgcagggcg cacgaaaact tgcgatgcct tcagcgaaat gccgataggc
     4321 cgcagaggat tgatcctgcg ctaaatcacc tgtcgcgaca atcaggtcga attcgtgctg
     4381 gtgtggacga atcgcctcca gcaccgcctg gtagctttcc caggtattta cccctaacag
     4441 ggcttcgtgc ttttgtgcaa acaggtgagt atcggtaatt tgtaaaatcc tgactctggc
     4501 ctcaccagcc agaggaaggg ttaacaggct ttccaaatgg tgtccttagg tttcacgacg
     4561 ctagtaaacc ggaatcgcca tcgctccatg tgctaaacag tatcgcaacc agtccgctaa
     4621 aaactggtta atttgatgct tttcgtcgcg ttgatgcaac tttttattag gataatcata
     4681 ccgcgctttg aagcgaaaaa tctgctggct tgaacacact tcagccacca tcgcgtcatg
     4741 atacagacgc accgtcattg acggaaggct ccagtaactg atcgcgggcg cagtctgttc
     4801 tattgtcacc agggtagtgt atcgggtcga ttccacaatc gtcagccgat attgtgcgtt
     4861 tgccacctga tagcttacag tttcgccggg tgcgtcattg cgcggtaaca aacggcgcaa
     4921 ttgtgaaaag ttcatctcgc acaggcgcat catttcagga aagtcaggtg tgtaacgctt
     4981 catttatgcc cactcatttt ttaacgcttg atgatgcagc tgcagccatt gcaaagcgat
     5041 gaccgacgct gcgttgtcga ttttcccctc ttctacccac tggtatgcct gttcccggct
     5101 taccacatga acgcgaatat cttcgttttc atcagccaga ccatgaatac cgcttgcggt
     5161 cgtggcgtcc acttcgccca ccataattga cgaacgctca ctggtgcccc ccgggcttgc
     5221 caggaaactt aacaccggtt tggtccgttt gactatcagt cccgcctctt caatcgcttc
     5281 gcgacgggca acatcttcca cactttcacc ctcttctatc atcccggcaa ccatctccag
     5341 tagccagggg gtttcgctgg tgtcgtacgc ggcaatccga atctgctcaa tcagcacaac
     5401 ttcatcacgc actgggtcaa agggtagcaa gactgcggcg tgaccgcgct caaaaatttc
     5461 ccgccgtacc tcatgactca tttgcccgtt gaatagacga tgacgaaatc tataaagatc
     5521 taatgaaaaa aagccgcgat aaagtgtttc tcgtgcaata atttctacat cgtttttgcc
     5581 aaatgtaacg ggcaggttgt ctggcttaag cattgttaat gtcctggcac taatagtgaa
     5641 ttaaatgtga atttcagcga cgtttgactg ccgtttgagc agtcatgtgt taaattgagg
     5701 cacattaacg ccctatggca cgtaacgcca accttttgcg gtagcggatt ctgctagaat
     5761 ccgcaataat tttacagttt gatcgcgcta aatactgctt caccacaagg aatgcaaatg
     5821 aagaaattgc tccccattct tatcggcctg agcctttctg ggttcagttc gttgagccag
     5881 gccgagaacc tgatgcaagt ttatcagcaa gcacgcctta gtaacccgga attgcgtaag
     5941 tctgccgccg atcgtgatgc tgcctttgaa aaaattaatg aagcgcgcag tccattactg
     6001 ccacagctag gtttaggtgc agattacacc tatagcaacg gctaccgcga cgcgaacggc
     6061 atcaactcta acgcgaccag tgcgtccctg cagttaactc aatccatttt tgatatgtcg
     6121 aaatggcgtg cgttaacgct gcaggaaaaa gcagcaggga ttcaggacgt cacatatcag
     6181 accgatcagc aaaccttgat cctcaacacc gcgaccgctt atttcaacgt gttgaatgct
     6241 attgacgttc tttcctatac acaggcacaa aaagaagcga tctaccgtca attagatcaa
     6301 accacccaac gttttaacgt gggcctggta gcgataaccg acgtgcagaa cgcccgcgcg
     6361 cagtacgata ccgtgctggc gaacgaagtg accgcacgca ataaccttga taacgcggta
     6421 gagcagctgc gccagatcac cggtaactac tatccagaac tggcagcgct gaatgtcgaa
     6481 aactttaaaa ccgacaaacc acagccggtt aacgcgctgc tgaaagaagc cgaaaaacgc
     6541 aacctgtcgc tgttacaggc acgcttgagc caggacctgg cgcgcgagca aattcgccag
     6601 gcgcaggatg gtcacttacc gacgctggat ttaacggctt ctaccgggat ttctgacacc
     6661 tcttatagcg gttcgaaaac ccgtggtgcc gctggtaccc agtatgacga cagcaatatg
     6721 ggccagaaca aagtgggcct gagcttctcg ctgccgattt atcagggcgg aatggttaac
     6781 tcgcaggtga aacaggcaca gtacaacttt gtcggtgcca gcgagcaact ggaaagcgcg
     6841 catcgtagcg tcgtgcaaac cgtacgttcc tccttcaaca acattaatgc atctatcagt
     6901 agcattaacg cctacaaaca agccgtagtt tccgctcaaa gctcattaga cgcgatggaa
     6961 gcgggctact cggtcggtac gcgtaccatt gttgatgtgt tggatgcaac caccacgctg
     7021 tacaacgcta agcaagagct ggcaaatgcg cgttataact acctgattaa tcagctgaat
     7081 attaagtcag ccctgggtac gttgaacgag caggatctgc tggcactgaa caatgcgctg
     7141 agcaaaccgg tttccactaa tccggaaaac gttgccccgc aaacgccgga acagaatgct
     7201 attgctgatg gttatgcgcc tgatagcccg gcacccgtcg ttcagcaaac atccgcacgc
     7261 actaccacca gtaacggtca taaccctttc cgtaactgat gacgacgacg gggcttcggc
     7321 cccgtctgaa cgtaaggcaa cgtaaagata cgggttatct gccgcattct tcccccttct
     7381 cgcttcaatt tcgaccagcc atcctctatt ctgatgggta tttaccactg gtcccggaag
     7441 acaaaaatga aacggacaaa atccatacgc cacgcatcgt tccgcaaaaa ctggagcgcg
     7501 cgccatctga caccagtcgc tctcgcggtt gccactgttt ttatgctggc tggctgtgaa
     7561 aagagtgatg aaacagtgtc tctctatcaa aatgctgacg actgttcagc tgcaaaccca
     7621 ggcaaaagcg ccgaatgtac caccgcgtac aacaatgcgc tgaaagaagc cgaacgtact
     7681 gcgccgaaat acgccacccg tgaagactgt gttgctgaat ttggtgaagg tcagtgccag
     7741 caggcaccag cccaggctgg catggcacca gaaaaccagg cgcaggccca gcaatccagc
     7801 gggagtttct ggatgccgct gatggccggt tacatgatgg ggcgtctgat gggcggcggc
     7861 gcgggatttg cacagcagcc gctgttctcc tcgaaaaacc cagccagccc ggcttacggt
     7921 aaatataccg acgcgacggg taaaaactat ggcgcagccc agccaggccg caccatgacc
     7981 gtaccgaaga cagccatggc accaaaaccg gcgaccacca ctaccgttac ccgtggcggt
     8041 tttggtgaat ctgttgccaa acaaagcact atgcagcgta gcgcaactgg cacctcttct
     8101 cgttcaatgg gtggctgata ccgatggaaa gagtcagtat taccgagcgc ccggactggc
     8161 gtgagaaagc ccacgaatac ggtttcaatt ttcacaccat gtacggcgag ccgtactggt
     8221 gtgaagatgc ttactacaag ttgaccctcg cccaggttga aaagctggaa gaagtcaccg
     8281 ccgaactgca ccagatgtgc ctgaaagtgg tggaaaaagt gatcgccagc gatgagctga
     8341 tgaccaaatt ccgcattcca aaacacacct ggagttttgt gcgccagtca tggctgacgc
     8401 accagccatc gctttattcg cgtcttgatc tggcgtggga tggcactggc gaacctaaac
     8461 ttctggaaaa taacgccgat acgccaacgt cactatacga ggcggcgttc tttcagtgga
     8521 tctggctgga agatcagctt aacgccggta acttgccgga gggcagcgac cagtttaaca
     8581 gtctgcaaga aaaactgatc gatcgcttcg ttgagctgcg tgaacagtat ggcttccagt
     8641 tgctgcatct cacctgctgt cgcgacacgg tggaagatcg cggaaccatt cagtatttgc
     8701 aggactgcgc aacggaagct gaaattgcta ctgagttcct ctacatcgat gatatcgggt
     8761 taggtgaaaa aggtcagttc acggatttac aggatcaggt aatttccaac ctgttcaagc
     8821 tgtatccgtg ggaatttatg ttgcgtgaga tgttttccac caagctggag gatgcaggcg
     8881 tacgctggct ggaaccggcg tggaagagca ttatctccaa caaggcactt ctaccgctac
     8941 tgtgggagat gttcccgaat cacccgaacc tgctgcccgc ttattttgcg gaagatgatc
     9001 atccgcaaat ggaaaaatat gtggttaaac cgatcttctc ccgtgaaggc gcaaacgtgt
     9061 cgatcattga aaacggcaaa accattgaag cagcggaagg tccgtatggc gaagaaggga
     9121 tgattgttca gcaattccac ccgttaccga aattcggcga cagctatatg ctgattggta
     9181 gctggctggt gaacgatcaa cccgccggaa ttggcattcg tgaagaccgt gcattgatca
     9241 cccaggatat gtctcggttt tatccacata tttttgttga gtaatcaatg atgccggatg
     9301 gcattcgcca tccggtaatt gttaacctat ctgcaccgac agcatactca ggctgcccat
     9361 ttctatacca tcgaccggaa tggtaattgg ctcctgccca tcccacgcac ctaagacata
     9421 caacaacggc agatagtgct ccggcgttgg gttcgataac gcgccacctt catggtcgag
     9481 gtaattcacc agaggatgct gctctaccgc cccttgccac gtcagattcg ctttcacata
     9541 ctcattaaac gacatcgccc aaggatatgg tgaactatca ccgtgccact tcactgtacg
     9601 caggttatgc accacgttgc cactggcgac caacattatt ccttcatctc gcagcgctgc
     9661 cagtttgcgc cccatttcga aatgccaggc ggcaggtttg ctactgtcga tactcaactg
     9721 caccatcggg atatcggcgt caggatacat cttaatcagc acgccccagg aaccgtggtc
     9781 aaagccccag gcttctttat ccagcgtcac cgggaccggt gctaacagct caaccagatg
     9841 ctgtgccagc acaggcgaac ccggagcagg ataatgcgta tcgtacagcg cctgcgggaa
     9901 gccaccaaag tcatgaatcg tgggcggcgt ctccatcgcg gtcactcctg ttccacgggt
     9961 aaaccagtga gccgaaacca ccacaatcgc ttgcgggcgt ggcaatgtca tccccaactt
    10021 ctgccagctg cgggtataca aattatcttc cagcacgttc atcggactac cgtgacctaa
    10081 aaacaatgct ggcatacgtg ttgaagacat gatgatatcc ttaactaaag gtgtcatttt
    10141 gatatcctca caatacgctt gttcggcgga gtaagaaccc ggataacaat gatgatgatc
    10201 atcagttatt ttgacgatct gcctgaaggt gaagatttat aaggagttgt cgatgtcagt
    10261 acctctcatt ctgaccatac tggcgggggc agccacgttt attggcgcgt ttctcggcgt
    10321 tctcgggcaa aaaccctcga accgcttact ggcgttttcg ctaggatttg cggcggggat
    10381 catgttgctc atctcattaa tggaaatgct tcctgccgca ctagcagctg aaggaatgtc
    10441 gcctgtgttg ggttatggaa tgtttatctt cggtctgctt ggctattttg gcctggaccg
    10501 catgttgcca catgcgcatc cgcaggattt aatgcaaaaa tcggtgcagc cgttgccaaa
    10561 atcgatcaag cgcacagcca ttctgctcac tctcggcatc agtctgcata acttcccgga
    10621 agggattgcc acctttgtca cggcgagcag caacctggag ctgggatttg gcatcgcact
    10681 ggccgtcgcg ttgcacaata ttcctgaagg tctggcggtt gcaggcccgg tttatgcagc
    10741 aacgggttct aaacgcacgg caattctgtg ggcggggatt tctggattgg cagaaattct
    10801 tggtggtgtg ctggcgtggt taatcctcgg tagcatgatt tcccccgtgg tcatggcggc
    10861 aatcatggcg gcggttgcag gaattatggt ggcgctctcg gttgatgaat taatgccgct
    10921 cgccaaagag attgacccta ataataaccc cagctatggc gtactatgtg gaatgtcagt
    10981 gatgggattc agtttagtgc tgctacaaac ggcgggaatt ggttaaaagc aacatcagaa
    11041 gttggccagc gcatggacaa cttctgctag cttcactaaa gccttattct accttttcgg
    11101 ctgtctcttc tctcgtactg tttaagtatt tgttccgcgc ttcaagcagt gcgtctctgt
    11161 cctggcgaaa cgcccgacaa taacgtttca aatgcaggaa ataacccacc acaacaagag
    11221 caatcaatac aatccagtac caggcgataa acatcctttt acccttaata tttaacaata
    11281 ctaaacattt agcgcataaa tttcacatat cctttttcgg atatattcat atggtcgcat
    11341 aataacggaa caattatatg attttgatca taatgaaaaa gaatgcctga tattaatgaa
    11401 attttctcat agaaagagaa gcggaattaa catcttgcat agcagggcaa aataaaaacc
    11461 gaagccagct acaacttcgg tttcttgatt aaggcagtaa ttaagcagcg gttttcagct
    11521 ggctttacgc tcatgtgcct gacggtatgc caccaggtct tcaatagtca cgagcgccat
    11581 attgtgttta ttggcaaact caatgcactc tggtgcacgc gccatcgtgc catcgtcatt
    11641 agtcaactca cacagcacac cagccggttt aaagcctgcc agcgtcatca aatcaatagt
    11701 cgcttcagta tgaccgccac gcgtcagtac accacctgcc tgcgcgcgaa gtgggaaaac
    11761 gtggcccggg cgattcagat cagaaggttt tgcgccatcg gcaatcgctg cgcgaacggt
    11821 cgtaattcga tcagcggcag aaacaccggt agtcacacct tcagccgctt caatggtcac
    11881 ggtaaaaccg gtgccatagg cgctggtgtt attttctacc atcattggca gatcaagttg
    11941 tttacggcga tcttcagtga tgcacaggca aacaataccg ctaccgtggc gaatggtcag
    12001 cgccatctgc tcaacggtca tggtttctgc cgggaagatc atatcgcctt cgttttcacg
    12061 gtcttcatca tcaagcacca ttacaccgcg tccttcacgc agcgcagcca gtgcattttc
    12121 aacacgttcg aaaggcgtac caaaagagga aagtagcgtc tgattcatgg taaaaaaacc
    12181 tcactaaaat tatggttacc agaatcaggg cagtcttagg agtggcggca tatagccaaa
    12241 ataacgtgag cgggtccatg cccgacagaa tcgttactct ctcccatccg gactctaacc
    12301 gtcggccccg gaattacacc ggatctgctg tcctttgagt tcgcacccaa agcgctcgcg
    12361 ggctttcaac tgagttgatt taccgccggt ggggaatttc gccccgccct gagaataagc
    12421 ggattcacta taacgctaat gattagcggc agcaacgcat agcttcacat aattctggtt
    12481 tatgacttac ccttatcgca ctacaatggc actcaacacc tatcagtaca gggaaactac
    12541 aatgattgac ccgaaaaaaa ttgagcaaat cgctcgccag gttcacgaat caatgcctaa
    12601 aggaatcagg gagttcgggg aagatgtgga gaaaaaaatc cgccaaaccc tacaagctca
    12661 gctgacgcgc ctcgaccttg taagccgtga agagttcgac gtccaaacgc aagtcctgtt
    12721 acggactcgt gagaaactgg cgctgcttga acaaagaatg agcgaactgg aaaatcgcag
    12781 tactgagatt aaaaagcagc cagatccaga gactctcccc ccaacgctgt aaacatttgt
    12841 ctttaatgca ctgtaaatta tgaattaatc ctctaattta cagt
//
LOCUS       DAZKHF020000125        12883 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2201S31241
            SAMN43267800-rid25106413.denovo.126, whole genome shotgun sequence.
ACCESSION   DAZKHF020000125 DAZKHF020000000
VERSION     DAZKHF020000125.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267800
            Sequence Read Archive: SRR30307263
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 12883)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12883)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 12883)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350581.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350581.2
            Genome Coverage       :: 151x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:21:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,652
            CDSs (total)                      :: 5,557
            Genes (coding)                    :: 5,320
            CDSs (with protein)               :: 5,320
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 237
            CDSs (without protein)            :: 237
            Pseudo Genes (ambiguous residues) :: 0 of 237
            Pseudo Genes (frameshifted)       :: 102 of 237
            Pseudo Genes (incomplete)         :: 130 of 237
            Pseudo Genes (internal stop)      :: 52 of 237
            Pseudo Genes (multiple problems)  :: 41 of 237
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12883
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267800-rid25106413.denovo.126"
                     /serotype="O 26:H11"
                     /isolate="2201S31241"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-01"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            105..1214
                     /gene="livK"
                     /locus_tag="ACHOSC_RS14345"
                     /old_locus_tag="pgaptmp_002869"
     CDS             105..1214
                     /gene="livK"
                     /locus_tag="ACHOSC_RS14345"
                     /old_locus_tag="pgaptmp_002869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="high-affinity branched-chain amino acid ABC
                     transporter substrate-binding protein LivK"
                     /protein_id="HGU4548773.1"
                     /translation="MKRNAKTIIAGMIALAISHTAMADDIKVAVVGAMSGPIAQWGDM
                     EFNGARQAIKDINAKGGIKGDKLVGVEYDDACDPKQAVAVANKIVNDGIKYVIGHLCS
                     SSTQPASDIYEDEGILMISPGATNPELTQRGYQHIMRTAGLDSSQGPTAAKYILETVK
                     PQRIAIIHDKQQYGEGLARSVQDGLKAANANVVFFDGITAGEKDFSALIARLKKENID
                     FVYYGGYYPEMGQMLRQARSVGLKTQFMGPEGVGNASLSNIAGDAAEGMLVTMPKRYD
                     QDPANQGIVDALKADKKDPSGPYVWITYAAVQSLATALERTGSDEPLALVKDLKANGA
                     NTVIGPLNWDEKGDLKGFDFGVFQWHADGSSTAAK"
     gene            1262..2188
                     /gene="livH"
                     /locus_tag="ACHOSC_RS14350"
                     /old_locus_tag="pgaptmp_002870"
     CDS             1262..2188
                     /gene="livH"
                     /locus_tag="ACHOSC_RS14350"
                     /old_locus_tag="pgaptmp_002870"
                     /EC_number="7.4.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010436488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="high-affinity branched-chain amino acid ABC
                     transporter permease LivH"
                     /protein_id="HGU4548774.1"
                     /translation="MSEQFLYFLQQMFNGVTLGSTYALIAIGYTMVYGIIGMINFAHG
                     EVYMIGSYVSFMIIAALMMMGIDTGWLLVAAGFVGAIVIASAYGWSIERVAYRPVRNS
                     KRLIALISAIGMSIFLQNYVSLTEGSRDVALPSLFNGQWVVGHSENFSASITTMQAVI
                     WIVTFLAMLALTIFIRYSRMGRACRACAEDLKMASLLGINTDRVIALTFVIGAAMAAV
                     AGVLLGQFYGVINPYIGFMAGMKAFTAAVLGGIGSIPGAMIGGLILGIAEALSSAYLS
                     TEYKDVVSFALLILVLLVMPTGILGRPEVEKV"
     gene            2185..3462
                     /gene="livM"
                     /locus_tag="ACHOSC_RS14355"
                     /old_locus_tag="pgaptmp_002871"
     CDS             2185..3462
                     /gene="livM"
                     /locus_tag="ACHOSC_RS14355"
                     /old_locus_tag="pgaptmp_002871"
                     /EC_number="7.4.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched chain amino acid ABC transporter
                     permease LivM"
                     /protein_id="HGU4548775.1"
                     /translation="MKPMHIAMALLSAAMFFVLAGVFMGVQLELDGTKLVVDTASDIR
                     WQWVFIGTAVVFFFQLLRPAFQKGLKSVSGPKFILPAIDGSTVKQKLFLVALLVLAVA
                     WPFMVSRGTVDIATLTMIYIILGLGLNVVVGLSGLLVLGYGGFYAIGAYTFALLNHYY
                     GLGFWTCLPIAGLMAAAAGFLLGFPVLRLRGDYLAIVTLGFGEIVRILLLNNTEITGG
                     PNGISQIPKPTLFGLEFSRTAREGGWDTFSNFFGLKYDPSDRVIFLYLVALLLVVLSL
                     FVINRLLRMPLGRAWEALREDEIACRSLGLSPRRIKLTAFTISAAFAGFAGTLFAARQ
                     GFVSPESFTFAESAFVLAIVVLGGMGSQFAVILAAILLVVSRELMRDFNEYSMLMLGG
                     LMVLMMIWRPQGLLPMTRPQLKLKNGAAKGEQA"
     gene            3459..4226
                     /gene="livG"
                     /locus_tag="ACHOSC_RS14360"
                     /old_locus_tag="pgaptmp_002872"
     CDS             3459..4226
                     /gene="livG"
                     /locus_tag="ACHOSC_RS14360"
                     /old_locus_tag="pgaptmp_002872"
                     /EC_number="7.4.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000084170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="high-affinity branched-chain amino acid ABC
                     transporter ATP-binding protein LivG"
                     /protein_id="HGU4548776.1"
                     /translation="MSQPLLSVNGLMMRFGGLLAVNNVNLELYPQEIVSLIGPNGAGK
                     TTVFNCLTGFYKPTGGTILLRDQHLEGLPGQQIARMGVVRTFQHVRLFREMTVIENLL
                     VAQHQQLKTGLFSGLLKTPSFRRAQSEALDRAATWLERIGLLEHANRQASNLAYGDQR
                     RLEIARCMVTQPEILMLDEPAAGLNPKETKELDELIAELRNHHNTTILLIEHDMKLVM
                     GISDRIYVVNQGTPLANGTPEQIRNNPDVIRAYLGEA"
     gene            4228..4941
                     /gene="livF"
                     /locus_tag="ACHOSC_RS14365"
                     /old_locus_tag="pgaptmp_002873"
     CDS             4228..4941
                     /gene="livF"
                     /locus_tag="ACHOSC_RS14365"
                     /old_locus_tag="pgaptmp_002873"
                     /EC_number="7.4.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312328.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="high-affinity branched-chain amino acid ABC
                     transporter ATP-binding protein LivF"
                     /protein_id="HGU4548777.1"
                     /translation="MEKVMLSFDKVSAHYGKIQALHEVSLHINQGEIVTLIGANGAGK
                     TTLLGTLCGDPRATSGRIVFDDKDITDWQTAKIMREAVAIVPEGRRVFSRMTVEENLA
                     MGGFFAERDQFQERIKWVYELFPRLHERRVQRAGTMSGGEQQMLAIGRALMSNPRLLL
                     LDEPSLGLAPIIIQQIFDTIEQLREQGMTIFLVEQNANQALKLADRGYVLENGHVVLS
                     DTGDALLANEAVRSAYLGG"
     gene            5337..6653
                     /gene="ugpB"
                     /locus_tag="ACHOSC_RS14370"
                     /old_locus_tag="pgaptmp_002874"
     CDS             5337..6653
                     /gene="ugpB"
                     /locus_tag="ACHOSC_RS14370"
                     /old_locus_tag="pgaptmp_002874"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sn-glycerol-3-phosphate ABC transporter
                     substrate-binding protein UgpB"
                     /protein_id="HGU4548778.1"
                     /translation="MKPLHYTASALALGLALMGNAQAVTTIPFWHSMEGELGKEVDSL
                     AQRFNAENPDYKIVPTYKGNYEQNLSAGIAAFRTGNAPAILQVYEVGTATMMASKAIK
                     PVYDVFKEAGIQFDESQFVPTVSGYYSDSKTGHLLSQPFNSSTPVLYYNKDAFKKAGL
                     DPEQPPKTWQDLADYAAKLKASGMKCGYASGWQGWIQLENFSAWNGLPFASKNNGFDG
                     TDAVLEFNKPEQVKHIAMLEEMNKKGDFSYVGRKDESTEKFYNGDCAMTTASSGSLAN
                     IREYAKFNYGVGMMPYDADAKDAPQNAIIGGASLWVMQGKDKETYTGVAKFLDFLAKP
                     ENAAEWHQKTGYLPITKAAYDLTREQGFYEKNPGADTATRQMLNKPPLPFTKGLRLGN
                     MPQIRVIVDEELESVWTGKKTPQQALDTAVERGNQLLRRFEKSTKS"
     gene            6751..7638
                     /gene="ugpA"
                     /locus_tag="ACHOSC_RS14375"
                     /old_locus_tag="pgaptmp_002875"
     CDS             6751..7638
                     /gene="ugpA"
                     /locus_tag="ACHOSC_RS14375"
                     /old_locus_tag="pgaptmp_002875"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000099274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sn-glycerol-3-phosphate ABC transporter permease
                     UgpA"
                     /protein_id="HGU4548779.1"
                     /translation="MSSSRPVFRSRWLPYLLVAPQLIITVIFFIWPAGEALWYSLQSV
                     DPFGFSSQFVGLDNFVALFHDSYYLDSFWTTIKFSTFVTVSGLLVSLFFAALVEYIVR
                     GSRFYQTLMLLPYAVAPAVAAVLWIFLFNPGRGLITHFLAEFGYDWNHAQNSGQAMFL
                     VVFASVWKQISYNFLFFYAALQSIPRSLIEAAAIDGAGPIRRFFKIALPLIAPVSFFL
                     LVVNLVYAFFDTFPVIDAATSGGPVQATTTLIYKIYREGFTGLDLASSAAQSVVLMFL
                     VIVLTVVQFRYVESKVRYQ"
     gene            7635..8480
                     /gene="ugpE"
                     /locus_tag="ACHOSC_RS14380"
                     /old_locus_tag="pgaptmp_002876"
     CDS             7635..8480
                     /gene="ugpE"
                     /locus_tag="ACHOSC_RS14380"
                     /old_locus_tag="pgaptmp_002876"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000572177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_process: GO:0015794 - glycerol-3-phosphate
                     transmembrane transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sn-glycerol-3-phosphate ABC transporter permease
                     UgpE"
                     /protein_id="HGU4548780.1"
                     /translation="MIENRPWLTIFSHTMLILGIAVILFPLYVAFVAATLDKQAVYAA
                     PMTLIPGTHLLENIHNIWVNGVGTNSAPFWRMLLNSFVMAFSITLGKITVSMLSAFAI
                     VWFRFPLRNLFFWMIFITLMLPVEVRIFPTVEVIANLKMLDSYAGLTLPLMASATATF
                     LFRQFFMTLPDELVEAARIDGASPMRFFCDIVFPLSKTNLAALFVITFIYGWNQYLWP
                     LLIITDVDLGTTVAGIKGMIATGEGTTEWNSVMAAMLLTLIPPVVIVLVMQRAFVRGL
                     VDSEK"
     gene            8482..9552
                     /gene="ugpC"
                     /locus_tag="ACHOSC_RS14385"
                     /old_locus_tag="pgaptmp_002877"
     CDS             8482..9552
                     /gene="ugpC"
                     /locus_tag="ACHOSC_RS14385"
                     /old_locus_tag="pgaptmp_002877"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417907.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sn-glycerol 3-phosphate ABC transporter ATP
                     binding protein UgpC"
                     /protein_id="HGU4548781.1"
                     /translation="MAGLKLQAVTKSWDGKTQVIKPLTLDVADGEFIVMVGPSGCGKS
                     TLLRMVAGLERVTEGDIWINDQRVTEMEPKDRGIAMVFQNYALYPHMSVEENMAWGLK
                     IRGMGKQQIAERVKEAARILELDGLLKRRPRELSGGQRQRVAMGRAIVRDPAVFLFDE
                     PLSNLDAKLRVQMRLELQQLHRRLKTTSLYVTHDQVEAMTLAQRVMVMNGGVAEQIGT
                     PVEVYEKPASLFVASFIGSPAMNLLTGRVNNEGTHFELDGGIELPLNGGYRQYAGRKM
                     TLGIRPEHIALSSQAEGGVPLVMDTLEILGADNLAHGRWGEQKLVVRLAHQERPTAGS
                     TLWLHLAENQLHLFDGETGQRV"
     gene            9549..10292
                     /gene="ugpQ"
                     /locus_tag="ACHOSC_RS14390"
                     /old_locus_tag="pgaptmp_002878"
     CDS             9549..10292
                     /gene="ugpQ"
                     /locus_tag="ACHOSC_RS14390"
                     /old_locus_tag="pgaptmp_002878"
                     /EC_number="3.1.4.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008081 - phosphoric diester hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase"
                     /protein_id="HGU4548782.1"
                     /translation="MSNWPYPRIVAHRGGGKLAPENTLAAIDVGAKYGHKMIEFDAKL
                     SKDGEIFLLHDDNLERTSNGWGVAGELNWQDLLRVDAGSWYSKAFKGEPLPLLSQVAE
                     RCREHGMMANIEIKPTTGTGPLTGKMVALAARQLWAGMTPPLLSSFEIDALEAAQLAA
                     PELPRGLLLDEWRDDWRELTARLGCVSIHLNHKLLDKVRVMQLKDAGLWILVYTVNKP
                     QRAAELLRWGVDCICTDAIDVIGPNFTAQ"
     gene            complement(10279..10719)
                     /locus_tag="ACHOSC_RS14395"
                     /old_locus_tag="pgaptmp_002879"
     CDS             complement(10279..10719)
                     /locus_tag="ACHOSC_RS14395"
                     /old_locus_tag="pgaptmp_002879"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2756 family protein"
                     /protein_id="HGU4548783.1"
                     /translation="MKRLLILTALLPFVGFAQPINTLNNPNQPGYQIPSQQRMQTQMQ
                     TQQIQQKGMLNQQLKTQTQLQQQHLENQINNNSQRVLQSQPGERNPARQQMLPNTNGG
                     MLNSNRNPDSSLNQQHMLPERRNGDMLNQPSTPQPDIPLKTIGP"
     gene            10839..12581
                     /gene="ggt"
                     /locus_tag="ACHOSC_RS14400"
                     /old_locus_tag="pgaptmp_002880"
     CDS             10839..12581
                     /gene="ggt"
                     /locus_tag="ACHOSC_RS14400"
                     /old_locus_tag="pgaptmp_002880"
                     /EC_number="2.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0036374 - glutathione hydrolase activity
                     [Evidence IEA];
                     GO_process: GO:0006751 - glutathione catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyltransferase"
                     /protein_id="HGU4548784.1"
                     /translation="MIKPTFLRRVAIAALLSGSCFSAAAAPPAPPVSYGVEEDVFHPV
                     RAKQGMVASVDATATQVGVDILKEGGNAVDAAVAVGYALAVTHPQAGNLGGGGFMLIR
                     SKNGNTTAIDFREMAPAKATRDMFLDDQGNPDSKKSLTSHLASGTPGTVAGFSLALDK
                     YGTMPLNKVVQPAFKLARDGFIVNDALADDLKTYGSEVLPNHENSKAIFWKEGEPLKK
                     GDTLVQANLAKSLEMIAENGPDEFYKGTIAEQIAQEMQKNGGLITKEDLAAYKAVERT
                     PISGDYRGYQVYSMPPPSSGGIHIVQILNILENFDMKKYGFGSADAMQIMAEAEKYAY
                     ADRSEYLGDPDFVKVPWQALTNKAYAKSIADQIDINKAKPSSEIRPGKLAPYESNQTT
                     HYSVVDKDGNAVAVTYTLNTTFGTGIVAGESGILLNNQMDDFSAKPGVPNVYGLVGGD
                     ANAVGPNKRPLSSMSPTIVVKDGKTWLVTGSPGGSRIITTVLQMVVNSIDYGMNVAEA
                     TNAPRFHHQWLPDELRVEKGFSPDTLKLLEAKGQKVALKEAMGSTQSIMVGPDGELYG
                     ASDPRSVDDLTAGY"
     gene            complement(12619..>12883)
                     /locus_tag="ACHOSC_RS14405"
                     /old_locus_tag="pgaptmp_002881"
     CDS             complement(12619..>12883)
                     /locus_tag="ACHOSC_RS14405"
                     /old_locus_tag="pgaptmp_002881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="YrhB family protein"
                     /protein_id="HGU4548785.1"
                     /translation="FAKANHYLDDADLPVVITLHGRFSQGWYFCFEAREFLETGDEAA
                     RLAGNAPFIIDKDSGEIHSLGTAKPLEEYLQDYEIKKATFGLP"
BASE COUNT         2834 a         3307 c         3719 g         3023 t
ORIGIN      
        1 cttaatcaga caggcaaaaa cagtgcagta taaaaaaaga acagtctgat ttgttaacac
       61 ataaaaacaa agcaacacaa catcacgaat ggggattttt gactatgaaa cggaatgcga
      121 aaactatcat cgcagggatg attgcactgg caatttcaca caccgctatg gctgacgata
      181 ttaaagtcgc cgttgtcggc gcgatgtccg gcccgattgc ccagtggggc gatatggaat
      241 ttaacggcgc gcgtcaggcg attaaagaca ttaatgccaa agggggaatt aagggcgaca
      301 agctggttgg cgtggaatat gacgacgcct gcgacccgaa acaagccgtt gcggtcgcca
      361 acaaaatcgt taatgacggc attaaatacg ttattggtca tctgtgttct tcttctaccc
      421 agcctgcgtc agatatctat gaagacgaag gtattctgat gatctcgccg ggagcgacca
      481 acccggagct gacccaacgc ggttatcaac acattatgcg tactgccggg ctggactctt
      541 cccaggggcc aacggcggca aaatacattc ttgagacggt gaagccccag cgcatcgcca
      601 tcattcacga caaacaacaa tatggcgaag ggctggcacg ttcggtgcag gacgggctga
      661 aagctgctaa cgccaatgtt gttttcttcg acggcattac cgctggcgag aaagatttct
      721 ccgcgctgat cgcccgcctg aaaaaagaaa acatcgactt cgtttactac ggcggttact
      781 acccggaaat ggggcagatg ctgcgccagg cccgttccgt tggcctgaaa acccagttta
      841 tggggccgga aggtgtgggt aatgcgtcgc tgtcgaatat tgctggcgat gctgccgaag
      901 gcatgttggt caccatgcca aaacgctatg accaggatcc ggcaaaccag ggcatcgttg
      961 atgcgctgaa agcagacaag aaagatccgt ccgggcctta tgtctggatc acttacgcgg
     1021 cggtgcaatc tctggcaaca gcccttgagc gtaccggcag cgatgagccg ctggcgctgg
     1081 tgaaagattt aaaagctaac ggtgcaaaca ccgtgattgg gccgctgaac tgggatgaaa
     1141 aaggcgatct taagggattt gattttggtg tcttccagtg gcacgccgac ggttcatcca
     1201 cggcagccaa gtgatcatcc caccgcccgt aaaatgcggg cgggtttaga aaggttacct
     1261 tatgtctgag cagtttttgt atttcttgca gcagatgttt aacggcgtca cgctgggcag
     1321 tacctacgcg ctgatagcca tcggctacac catggtttac ggcattatcg gcatgataaa
     1381 cttcgcccac ggcgaggttt atatgattgg cagctacgtc tcatttatga tcatcgccgc
     1441 gctgatgatg atgggcattg ataccggctg gctgctggtg gcggcgggat tcgtcggcgc
     1501 aatcgtcatt gccagcgcct acggctggag tatcgaacgg gtggcctatc gcccggtgcg
     1561 taactctaag cgcctgattg cgctcatctc ggcaatcggt atgtccatct tcctgcaaaa
     1621 ctacgtcagc ctgaccgaag gttcgcgtga cgtggcgctg ccgagcctgt ttaacggtca
     1681 gtgggtggtg ggccatagcg aaaacttctc tgcctctatt actaccatgc aggcggtgat
     1741 ctggattgtc accttcctcg ccatgctggc gctgacgatt ttcattcgct attcccgtat
     1801 ggggcgcgcg tgtcgtgcct gcgcggaaga tctgaaaatg gcgagtctgc ttggcattaa
     1861 caccgaccgg gtgattgcgc tgacctttgt gattggcgcg gcgatggcgg cggtggcggg
     1921 tgtgctgctc ggtcagttct acggcgtaat taacccctac atcggcttta tggccgggat
     1981 gaaagccttt accgctgcgg tgctcggtgg gattggcagc attccagggg cgatgattgg
     2041 tgggctgatc ctggggattg cggaggcgct ctcttctgcc tatctgagta cggaatataa
     2101 ggatgtggtt tcatttgccc tgctgattct ggtgctgctg gtgatgccga ccggtattct
     2161 gggtcgcccg gaggtagaga aagtatgaaa ccgatgcata ttgcaatggc gctgctctct
     2221 gccgcgatgt tttttgtcct ggcgggcgtc tttatgggcg tgcaactgga gctggatggc
     2281 accaaactgg tggtcgacac ggcttcagac atccgttggc agtgggtgtt tatcggcacg
     2341 gcggtggtct ttttcttcca gcttttgcga ccggctttcc agaaagggct aaaaagcgtt
     2401 tccggaccga agtttattct gcccgcgatt gatggctcca cggtgaagca gaaactattc
     2461 ctcgtggcgc tgctggtgct tgcggtggcg tggccgttta tggtgtcgcg tgggacggtg
     2521 gatatcgcca ccctgaccat gatctacatt atcctcggcc tcgggctgaa cgtggttgtt
     2581 ggtctttctg gtctgctggt gctggggtac ggcggctttt acgccatcgg cgcttacact
     2641 tttgcgctgc tcaatcacta ttacggcctg ggcttctgga cctgtctgcc gattgccggg
     2701 ttaatggcag cggcggccgg cttcctgctc ggcttcccgg tgcttcgctt gcgcggcgac
     2761 tatctggcga tcgttaccct cggtttcggc gaaattgtgc gcatattgct gctcaataac
     2821 accgaaatta ccggcggccc gaacggaatc agtcagatcc cgaaaccgac actcttcgga
     2881 ctcgagttca gccgtaccgc tcgtgaaggc ggctgggaca cgttcagtaa tttctttggc
     2941 ctgaaatacg atccctccga tcgtgtcatc ttcctctacc tggtggcgtt gctgctggtg
     3001 gtgctaagcc tgtttgtcat taaccgcctg ctgcggatgc cgctggggcg tgcgtgggaa
     3061 gcgttgcgtg aagacgagat cgcctgccgt tcgctgggct taagcccgcg tcgtatcaag
     3121 ctgactgcct ttaccataag tgccgcgttt gccggttttg ccggaacgct gtttgctgcg
     3181 cgccagggct ttgtcagccc ggaatccttc acctttgctg aatcggcgtt tgtactggcg
     3241 atagtggtgc tcggcggtat gggatcgcag tttgcggtga ttctggcggc aattttgctg
     3301 gtggtatcgc gcgaattgat gcgtgatttc aacgaataca gcatgttaat gctcggtggt
     3361 ttgatggtgc tgatgatgat ctggcgtccg cagggcttgc tgcccatgac gcgcccgcaa
     3421 ctgaagctga aaaacggcgc agcgaaagga gagcaggcat gagtcagcca ttattatctg
     3481 ttaacggtct gatgatgcgc ttcggcggct tgctggcggt gaacaacgtc aatcttgaac
     3541 tgtacccgca ggagatcgtc tcgttaatcg gccctaacgg tgccggaaaa accacggttt
     3601 ttaactgcct gaccggattc tacaaaccca ccggcggcac tattttactg cgtgatcagc
     3661 atctggaagg tttaccgggg cagcaaattg cccgcatggg cgtggtgcgt accttccagc
     3721 atgtgcgtct gttccgtgaa atgacggtga ttgaaaacct gctggtggcg cagcatcaac
     3781 aactgaaaac cgggctgttc tctggcctgt tgaaaacgcc ttctttccgt cgcgcccaga
     3841 gcgaagcgct cgaccgcgcc gccacctggc ttgagcgcat tggtttgctg gaacacgcca
     3901 accgtcaggc gagtaacctg gcctatggtg accaacgccg tctggagatt gcccgctgca
     3961 tggtgacgca gccggagatt ttaatgctcg acgaaccggc ggcaggtctt aacccaaaag
     4021 aaaccaaaga gctggatgag ctgattgccg aactgcgcaa tcatcacaac accactatct
     4081 tgttgattga acacgatatg aagctggtga tggggatttc agaccgaatt tatgtggtca
     4141 atcaggggac gccactggca aacggtacgc cggagcaaat ccgtaataac ccggacgtga
     4201 tccgtgccta tttaggtgag gcataagatg gaaaaagtca tgttgtcctt tgacaaagtc
     4261 agcgcccact acggcaaaat ccaggcgctg catgaggtga gcctgcatat caatcagggc
     4321 gagattgtca ccctcattgg cgcgaacggg gcggggaaaa ccaccttgct cggcacgtta
     4381 tgcggcgatc cgcgcgctac cagcgggcga attgtgtttg atgataaaga cattaccgac
     4441 tggcagacgg cgaaaatcat gcgcgaagcg gtagcgattg tcccggaagg gcgtcgcgtc
     4501 ttctcgcgga tgacggtgga agagaacctg gcgatgggcg gcttttttgc cgaacgcgac
     4561 cagttccagg agcgcataaa gtgggtgtat gaactgtttc cacgtctgca tgagcgccgt
     4621 gttcagcggg caggcaccat gtccggcggt gagcaacaaa tgctggcgat tggtcgcgcg
     4681 ctgatgagca acccgcgttt gctgctactt gatgagccat cgctcggtct tgcgccgatt
     4741 atcatccagc aaattttcga caccatcgag cagctgcgcg agcaggggat gactatcttc
     4801 ctcgtcgagc agaacgccaa ccaggcgcta aagctggcgg atcgcggcta cgtgctggaa
     4861 aacggccatg tggtgctctc cgataccggc gacgcgctgc tggcgaacga agcagtgaga
     4921 agtgcgtatt taggcgggta ataacacgtt gattgataag gagtcaaaag actcctttga
     4981 gacaggtgac aaatgtaaaa ttgcctgatg cgctgcgctt atcaggccta ctgggtgagt
     5041 tgcaatatat tgaatttgca cgatcttgta ggccggataa ggcgtttacg ccgcatccgg
     5101 catgaaacga tgggtaatct gtagagtttg attcagacct tctattttcc gcttatccgt
     5161 gccccatctc ccattttccc tcacccacgc cgtcaccgcc ttgtcatctt tctgacacct
     5221 tactatctta caaatgtaac aaaaaagtta tttttctgta attcgagcat gtcatgttac
     5281 cccgcgagca taaaacgcgt gaattcgcgc attcggtaca acaagagaga taaacgatga
     5341 aaccgttaca ttatacagct tcagcactgg cgctcggact ggcgttaatg gggaatgcac
     5401 aggcagtgac gaccattccg ttctggcatt ctatggaagg ggaactgggt aaagaggtgg
     5461 attctctggc ccaacgtttt aacgccgaaa atccggatta caaaattgta ccgacctata
     5521 aaggcaacta cgaacagaat ttaagcgcgg ggattgccgc atttcgtacc ggcaacgctc
     5581 cggctatttt gcaggtttat gaagttggca ccgccaccat gatggcatcg aaagccatta
     5641 aaccggtata tgacgtgttt aaagaggcgg ggattcaatt cgatgagtcg cagtttgtgc
     5701 cgacggtttc cggttactac tccgacagca aaacgggcca cttactctcc cagccattca
     5761 acagctcgac ccccgttctc tattacaaca aagacgcctt caagaaagca ggattagacc
     5821 cggaacaacc gccgaaaacc tggcaggatc tggcggacta tgccgcgaaa ctgaaagcct
     5881 ccggtatgaa gtgcggctac gccagcggct ggcagggctg gatccaactg gaaaacttta
     5941 gcgcctggaa cggtctgccg tttgccagca aaaacaacgg ctttgacggc acggacgcgg
     6001 tgctggagtt caacaagccg gagcaggtga aacatatcgc catgctcgag gagatgaaca
     6061 agaagggcga cttcagctac gtcggtcgta aggatgaatc caccgagaag ttctataacg
     6121 gtgattgcgc gatgaccacc gcctcttccg gttctcttgc caacattcgc gagtacgcca
     6181 aatttaatta cggcgtaggc atgatgcctt acgacgccga tgcgaaagat gcgccacaaa
     6241 acgccattat cggcggagcc agcctgtggg tgatgcaggg taaagataaa gaaacgtata
     6301 ccggtgtggc gaagttcctc gatttcctcg cgaagccaga aaacgctgcc gagtggcatc
     6361 agaaaaccgg ttatctgcca attaccaaag cggcgtatga cctgacccgt gagcagggct
     6421 tttacgagaa aaacccaggt gcggataccg cgacgcgtca gatgctgaat aagccgccgt
     6481 tgccgttcac caaagggctg cgtctgggca acatgccgca gatccgcgtg attgtggatg
     6541 aagagctgga gagcgtgtgg accggtaaga agacaccaca gcaggcactg gataccgccg
     6601 ttgagcgtgg aaatcagttg ctgcgccgct ttgagaaatc gacgaagtct taatcagtgt
     6661 aatgtcggat gcgtttcgct tatctgactt ggcatcgcgt gtaggccgga taagcgaagc
     6721 gcatccggca cagttcagga attaaccgta atgtcatcat cccgtccggt gttccgctcg
     6781 cgctggctgc cttatctgct ggtcgcgccg cagctcatca tcaccgttat cttttttatc
     6841 tggcctgcgg gcgaagcgtt gtggtactcg ctacaaagcg tcgatccgtt tggtttctcc
     6901 agccagtttg tcggcctgga taacttcgtc gcgctgtttc atgacagcta ctatctcgac
     6961 tccttctgga cgacgataaa attcagcacc tttgtcaccg tcagcggttt gctggtgtcg
     7021 ctgttcttcg cggcgctggt ggagtacatc gtgcgcggca gccgtttcta tcaaacctta
     7081 atgctgctgc cttacgccgt ggctcccgcc gttgccgccg tattgtggat cttcctgttt
     7141 aaccccggtc gcgggctgat cacccatttt ctcgcggagt tcggctacga ctggaaccac
     7201 gcgcaaaaca gcggtcaggc aatgtttctg gtggtgtttg cctcagtatg gaagcaaatc
     7261 agctacaact tcctgttctt ctatgccgcg ctgcaatcca ttccccgttc gttgatcgaa
     7321 gccgcagcca tcgacggtgc aggcccaatt cgccgcttct ttaagattgc gctgccgctt
     7381 atcgctccgg tgagtttctt cctgctggtg gtgaacctgg tgtatgcctt cttcgacacc
     7441 ttcccggtga tcgacgccgc cacgtccggc gggccggttc aggccaccac gacgctgatt
     7501 tataagatct accgtgaagg ctttaccgga ctggatctgg cttcgtctgc cgcgcagtcg
     7561 gtggtgttga tgttcctcgt catcgtgctg acggtggtgc agttccgcta tgttgaaagc
     7621 aaggtgcgtt accaatgatt gagaaccgtc cgtggctgac gatattcagc cataccatgc
     7681 tgatcctcgg gatcgcggtg atcctcttcc cgctgtacgt ggcgtttgtc gcggcgacgc
     7741 tggataaaca ggccgtctat gccgcgccga tgacgctcat ccccggcaca catctgctgg
     7801 aaaatatcca caacatctgg gttaacgggg taggcacgaa tagcgcgccg ttctggcgga
     7861 tgttgcttaa cagctttgtg atggcgttca gcattacgct cggcaaaatt accgtctcga
     7921 tgctctcggc atttgccatt gtctggtttc gttttccgct acgtaacctc ttcttctgga
     7981 tgatttttat caccctgatg ctgccggttg aagtacgtat cttcccgacg gtggaagtca
     8041 tcgccaacct gaagatgctc gacagctacg ccggtttaac gctgccgctg atggcctcgg
     8101 cgaccgccac gtttctgttt cgccagttct ttatgacgct gccggatgaa ctggtggaag
     8161 cggcgcggat cgacggcgcg tcaccaatgc gtttcttttg cgacatcgtt tttccgctct
     8221 ccaaaaccaa tctggcggcg ctgtttgtga tcacctttat ttacggctgg aaccagtatt
     8281 tgtggccgtt gttgattatt accgatgtgg atctcggcac caccgtggca gggatcaaag
     8341 ggatgatcgc tacaggcgaa ggcaccactg aatggaactc agtgatggcg gcgatgttgt
     8401 taacgcttat ccctccggtg gtgattgttt tagtgatgca gcgtgccttc gtgcgcggcc
     8461 tggtcgatag tgagaaataa gatggcagga ctgaaattac aggcagtaac caaaagctgg
     8521 gatggtaaaa cccaggtcat taaaccgctg acccttgatg tggcggatgg cgaatttatc
     8581 gtgatggtcg ggccgtctgg ctgcgggaaa tcgacgctgc tgcgcatggt tgccgggctg
     8641 gagcgggtga cagaaggcga tatctggatc aacgaccagc gcgtgactga aatggagcca
     8701 aaagatcgcg ggattgcgat ggtgttccag aactacgcgc tttatccgca tatgagtgtc
     8761 gaagaaaaca tggcgtgggg gctgaaaatt cgcggcatgg gcaagcagca aattgccgag
     8821 cgcgttaaag aagcggcgcg cattctggag ctggacggtc tgctcaaacg tcgcccgcgc
     8881 gagctttccg gcggtcagcg ccagcgtgtg gcgatgggcc gcgcgattgt gcgcgatccg
     8941 gcggtgttcc tgtttgatga gccgctctct aacctcgatg ccaagctgcg cgtgcagatg
     9001 cgtcttgaac tgcaacagtt gcaccgtcgc ctgaaaacga cttcactcta cgttactcac
     9061 gatcaggttg aagcgatgac gctcgcccag cgagtaatgg tgatgaacgg cggcgttgcc
     9121 gaacagattg gcacaccagt tgaagtctac gaaaagcccg ccagcctgtt tgtagcgagt
     9181 tttatcggca gtccggcgat gaacctgctg acaggccgcg tgaataacga aggcacgcat
     9241 ttcgaactgg acggcggtat tgagctgccg ctaaacggtg gctaccgtca gtatgccggg
     9301 cgtaaaatga ctctcggcat tcgcccggaa catattgcgc taagctcgca ggcagaaggc
     9361 ggcgtaccgc tggtgatgga cacgctggag atcctcggcg cagataacct ggcgcacgga
     9421 cgctggggcg agcagaagct ggtggtgcga ctggcgcatc aggagcgccc gacggcaggc
     9481 agcacgctgt ggctgcatct ggcggaaaat cagctgcatc tttttgatgg tgaaacagga
     9541 caacgagtat gagtaactgg ccttatcccc gcatcgtcgc tcatcgtggc ggcggtaagc
     9601 tggccccgga aaacaccctg gcggcaatcg acgtcggggc aaaatacggt cataagatga
     9661 tcgaatttga cgcgaagtta tcgaaagatg gcgagatctt cctgctccat gacgacaatc
     9721 tcgaacgcac cagcaacggc tggggcgtcg cgggtgaact gaactggcag gatttactgc
     9781 gcgtggatgc gggcagttgg tacagcaaag cgtttaaagg tgagccgctg ccgttgcttt
     9841 cgcaggtggc ggaacgctgt cgcgaacacg ggatgatggc gaatatcgaa atcaaaccca
     9901 ccactggcac tggaccatta acgggcaaaa tggtggcgct ggcggcacgc caactgtggg
     9961 ccggtatgac gccgccgctg ctgtcatcgt ttgagattga tgctttagaa gctgcacagc
    10021 tggcggcacc ggaactgccg cgtggtttat tgcttgatga gtggcgcgac gactggcgcg
    10081 aactgaccgc gcggctgggc tgcgtctcta ttcatctcaa tcataagtta ctcgataaag
    10141 tgcgagtgat gcagttgaaa gacgccggac tgtggattct ggtttatacc gtcaataaac
    10201 cccagcgcgc ggcagagttg ctgcgctggg gcgtggattg catctgtacc gatgcgattg
    10261 acgtgattgg tccgaacttt acggcccaat agttttcaac ggaatgtcag gctgcggcgt
    10321 gctgggctga ttcagcatgt cgccgtttct cctctccggc agcatatgct gctgattcaa
    10381 cgaactatcc ggattacggt tgctgtttaa catcccgccg ttggtgttgg gcagcatttg
    10441 ctgccgggcg ggatttcgct cccccggctg tgactgcaac acccgctgag aattattgtt
    10501 tatctggttt tctaaatgct gctgttgcaa ctgagtttgc gttttcagtt gctgattcag
    10561 catccctttt tgctggattt gctgagtctg catctgggtt tgcatccgct gctggctggg
    10621 tatctgatac cccggctggt tagggttgtt cagagtatta atgggctgtg caaagccgac
    10681 aaacggcagg agtgccgtaa gaatcagaag ccgtttcatc gcgtatcctc ctctgaagat
    10741 atcctttaag tttactcgct tcccgacaaa acgatgatta attcagagtt atataccagg
    10801 cttagctggg gttgcccctt aatctctgga gaataacgat gataaaaccg acgtttttac
    10861 gccgggtggc cattgctgct ctgctctcag gaagttgttt tagcgccgcc gccgcgcctc
    10921 ctgcgccgcc cgtctcgtat ggtgtggagg aagatgtctt ccacccggta cgcgcgaaac
    10981 agggaatggt agcgtctgtg gacgccactg ccactcaggt gggggtggat attctcaagg
    11041 agggcgggaa tgccgttgat gccgccgtgg cggtgggcta cgcgctggcg gtaacgcatc
    11101 cgcaggcagg gaatctgggc ggtggtggtt ttatgctgat ccgctcgaaa aatggcaata
    11161 ccaccgctat cgatttccgc gaaatggcac ccgctaaagc gacccgcgat atgttccttg
    11221 atgaccaggg caacccggac agcaaaaaat cactcacttc gcatctggct tccggcacac
    11281 caggtacggt cgctggtttc tcgctggcgt tggataagta cggcactatg ccgttgaaca
    11341 aagtggtgca gcccgcgttc aaactggcac gcgatggttt tatcgttaac gatgctttgg
    11401 cagacgatct caaaacctac ggtagcgaag tgttgccgaa tcacgaaaac agcaaagcta
    11461 tcttctggaa agagggcgaa ccgctgaaaa agggcgacac gctggtgcag gcgaacctgg
    11521 caaagagcct ggagatgatt gctgaaaacg gcccggacga attctataaa ggcacgattg
    11581 cggaacagat cgcccaggag atgcagaaaa acggtggctt gatcactaaa gaagatttag
    11641 cggcctataa agcggtcgaa cgcactccga taagcggcga ttatcgcggg tatcaggttt
    11701 actccatgcc accgccatcc tccggcggga tccatatcgt acaaatcctc aatattctgg
    11761 aaaacttcga tatgaagaaa tacggctttg gcagcgccga tgcgatgcaa atcatggcag
    11821 aagcggagaa atacgcctac gccgaccgct cggaatatct tggcgacccg gattttgtca
    11881 aagtcccgtg gcaggcactg accaataaag cctatgccaa atccattgcc gatcaaattg
    11941 atatcaacaa agcgaagccg tccagtgaga ttcgccctgg caagcttgcg ccttatgaga
    12001 gtaatcaaac tacccattac tctgtagtgg ataaagacgg taacgcggtg gcggtgacct
    12061 atacgctgaa caccaccttc ggtacgggta ttgtcgcggg cgagagcggt attctgctta
    12121 ataaccagat ggatgatttc tccgccaaac cgggcgtacc gaacgtttac gggctggtgg
    12181 gcggtgatgc caacgccgtc gggccgaaca aacgcccgct gtcgtcgatg tcgccgacca
    12241 ttgtggtgaa agacggtaaa acctggctgg ttaccggtag cccaggcggt agccggatca
    12301 tcactacagt gctgcaaatg gtggtgaata gcatcgatta tggcatgaac gtcgccgaag
    12361 cgaccaatgc gccgcgtttc caccatcagt ggttgccgga cgagctgcgt gtcgaaaaag
    12421 ggtttagccc ggatacgctc aagctgctgg aagcaaaagg tcagaaagtg gcgctgaaag
    12481 aggcgatggg cagtacacaa agcattatgg ttgggccgga cggtgagttg tacggcgcat
    12541 ccgacccgcg ctcggtggat gatttaacgg cggggtacta aggttagcgg ccctcttcgt
    12601 gggaagaggg ctattttgtc agggcaagcc gaaggtagcc ttttttattt cgtaatcctg
    12661 tagatattct tccagcggct ttgccgttcc cagagaatga atttcaccac tgtctttatc
    12721 aataataaaa ggtgcgttac cagctaagcg cgcggcctca tctccagttt cgagaaattc
    12781 tcgtgcttcg aaacagaaat accagccctg gctaaagcgt ccatgtagag taatgacgac
    12841 cgggagatct gcatcatcaa ggtaatggtt cgctttcgcg aat
//
LOCUS       DAZKHF020000126        12772 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2201S31241
            SAMN43267800-rid25106413.denovo.127, whole genome shotgun sequence.
ACCESSION   DAZKHF020000126 DAZKHF020000000
VERSION     DAZKHF020000126.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267800
            Sequence Read Archive: SRR30307263
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 12772)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12772)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 12772)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350581.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350581.2
            Genome Coverage       :: 151x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:21:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,652
            CDSs (total)                      :: 5,557
            Genes (coding)                    :: 5,320
            CDSs (with protein)               :: 5,320
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 237
            CDSs (without protein)            :: 237
            Pseudo Genes (ambiguous residues) :: 0 of 237
            Pseudo Genes (frameshifted)       :: 102 of 237
            Pseudo Genes (incomplete)         :: 130 of 237
            Pseudo Genes (internal stop)      :: 52 of 237
            Pseudo Genes (multiple problems)  :: 41 of 237
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12772
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267800-rid25106413.denovo.127"
                     /serotype="O 26:H11"
                     /isolate="2201S31241"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-01"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            144..710
                     /gene="yqaB"
                     /locus_tag="ACHOSC_RS14410"
                     /old_locus_tag="pgaptmp_002882"
     CDS             144..710
                     /gene="yqaB"
                     /locus_tag="ACHOSC_RS14410"
                     /old_locus_tag="pgaptmp_002882"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-1-phosphate/6-phosphogluconate
                     phosphatase"
                     /protein_id="HGU4548786.1"
                     /translation="MYERYAGLIFDMDGTILDTEPTHRKAWREVLGHYGLQYDVQAMI
                     ALNGSPTWRIAQAIIELNQADLDPHALAREKTEAVRSMLLDSVEPLPLVEVVKSWHGR
                     RPMAVGTGSESAIAEALLAHLGLRRYFDAVVAADHVKYHKPAPDTFLLCAQRMGVQPT
                     QCVVFEDADFGIQAARAAGMDAVDVRLL"
     gene            707..1135
                     /locus_tag="ACHOSC_RS14415"
                     /old_locus_tag="pgaptmp_002883"
     CDS             707..1135
                     /locus_tag="ACHOSC_RS14415"
                     /old_locus_tag="pgaptmp_002883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YqaA family protein"
                     /protein_id="HGU4548787.1"
                     /translation="MSEALSLFSLFASSFLSATLLPGNSEVVLVAMLLSGISYPWVLV
                     LTATMGNSLGGLTNVILGRFFPLRKTSRWQEKATGWLKRYGAVTLLLSWMPVVGDLLC
                     LLAGWMRISWGPVIFFLCLGKALRYVAVAAATVQGMMWWH"
     gene            1208..2764
                     /gene="gshA"
                     /locus_tag="ACHOSC_RS14420"
                     /old_locus_tag="pgaptmp_002884"
     CDS             1208..2764
                     /gene="gshA"
                     /locus_tag="ACHOSC_RS14420"
                     /old_locus_tag="pgaptmp_002884"
                     /EC_number="6.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004357 - glutamate-cysteine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0006750 - glutathione biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--cysteine ligase"
                     /protein_id="HGU4548788.1"
                     /translation="MIPDVSQALAWLEKHPQALKGIQRGLERETLRVNADGTLATTGH
                     PEALGSALTHKWITTDFAEALLEFITPVDGDIEHMLTFMRDLHRYTARNMGDERMWPL
                     SMPCYIAEGQDIELAQYGTSNTGRFKTLYREGLKNRYGALMQTISGVHYNFSLPMAFW
                     QAKCGDISGADAKEKISAGYFRVIRNYYRFGWVIPYLFGASPAICSSFLQGKPTSLPF
                     EKTECGMYYLPYATSLRLSDLGYTNKSQSNLGITFNDLYEYVAGLKQAIKTPSEEYAK
                     IGIEKDGKRLQINSNVLQIENELYAPIRPKRVTRSGESPSDALLRGGIEYIEVRSLDI
                     NPFSPIGVDEQQVRFLDLFMVWCALADAPEMSSSELACTRVNWNRVILEGRKPGLTLG
                     IGCETAQFPLLQVGKDLFRDLKRVAQTLDSINGGEAYQKVCDELVACFDNPDLTFSAR
                     ILRSMIDTGIGGTGKAFAEAYRNLLREEPLEILREEDFVAEREASERRQQEMETADTE
                     PFAVWLEKHA"
     gene            2914..3429
                     /gene="luxS"
                     /locus_tag="ACHOSC_RS14425"
                     /old_locus_tag="pgaptmp_002885"
     CDS             2914..3429
                     /gene="luxS"
                     /locus_tag="ACHOSC_RS14425"
                     /old_locus_tag="pgaptmp_002885"
                     /EC_number="4.4.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008458247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-ribosylhomocysteine lyase"
                     /protein_id="HGU4548789.1"
                     /translation="MPLLDSFTVDHTRMEAPAVRVAKTMNTPHGDAITVFDLRFCVPN
                     KEVMPERGIHTLEHLFAGFMRNHLNGKGVEIIDISPMGCRTGFYMSLIGTPDEQRVAD
                     AWKAAMEDVLKVQDQNQIPELNVYQCGTYQMHSLQEAQDIARSILERDVRINSNEELA
                     LPKEKLQELHI"
     gene            complement(3493..5031)
                     /gene="emrB"
                     /locus_tag="ACHOSC_RS14430"
                     /old_locus_tag="pgaptmp_002886"
     CDS             complement(3493..5031)
                     /gene="emrB"
                     /locus_tag="ACHOSC_RS14430"
                     /old_locus_tag="pgaptmp_002886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter permease
                     subunit EmrB"
                     /protein_id="HGU4548790.1"
                     /translation="MQQQKPLEGAQLVIMTIALSLATFMQVLDSTIANVAIPTIAGNL
                     GSSLSQGTWVITSFGVANAISIPLTGWLAKRVGEVKLFLWSTIAFAIASWACGVSSSL
                     NMLIFFRVIQGIVAGPLIPLSQSLLLNNYPPAKRSIALALWSMTVIVAPICGPILGGY
                     ISDNYHWGWIFFINVPIGVAVVLMTLQTLRGRETRTERRRIDAVGLALLVIGIGSLQI
                     MLDRGKELDWFSSQEIIILTVVAVVAICFLIVWELTDDNPIVDLSLFKSRNFTIGCLC
                     ISLAYMLYFGAIVLLPQLLQEVYGYTATWAGLASAPVGIIPVILSPIIGRFAHKLDMR
                     RLVTFSFIMYAVCFYWRAYTFEPGMDFGASAWPQFIQGFAVACFFMPLTTITLSGLPP
                     ERLAAASSLSNFTRTLAGSIGTSITTTMWTNRESMHHAQLTESVNPFNPNAQAMYSQL
                     EGLGMTQQQASGWIAQQITNQGLIISANEIFWMSAGIFLVLLGLVWFAKPPFGAGGGG
                     GGAH"
     gene            complement(5048..6220)
                     /gene="emrA"
                     /locus_tag="ACHOSC_RS14435"
                     /old_locus_tag="pgaptmp_002887"
     CDS             complement(5048..6220)
                     /gene="emrA"
                     /locus_tag="ACHOSC_RS14435"
                     /old_locus_tag="pgaptmp_002887"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter periplasmic
                     adaptor subunit EmrA"
                     /protein_id="HGU4548791.1"
                     /translation="MSANAETQTPQQPVKKSGKRKRLLLLLTLLFIIIAVAIGIYWFL
                     VLRHFEETDDAYVAGNQIQIMSQVSGSVTKVWADNTDFVKEGDVLVTLDPTDARQAFE
                     KAKTALASSVRQTHQLMINSKQLQANIEVQKIALAKAQSDYNRRVPLGNANLIGREEL
                     QHARDAVTSAQAQLDVAIQQYNANQAMILGTKLEDQPAVQQAATEVRNAWLALERTRI
                     VSPMTGYVSRRAVQPGAQISPTTPLMAVVPATNMWVDANFKETQIANMRIGQPVTITT
                     DIYGDDVKYTGKVVGLDMGTGSAFSLLPAQNATGNWIKVVQRLPVRIELDQKQLEQYP
                     LRIGLSTLVSVNTTNRDGQVLANKVRSTPVAVSTAREISLAPVNKLIDDIVKANAG"
     gene            complement(6347..6877)
                     /gene="emrR"
                     /locus_tag="ACHOSC_RS14440"
                     /old_locus_tag="pgaptmp_002888"
     CDS             complement(6347..6877)
                     /gene="emrR"
                     /locus_tag="ACHOSC_RS14440"
                     /old_locus_tag="pgaptmp_002888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux transporter EmrAB
                     transcriptional repressor EmrR"
                     /protein_id="HGU4548792.1"
                     /translation="MDSSFTPIEQMLKFRASRHEDFPYQEILLTRLCMHMQSKLLENR
                     NKMLKAQGINETLFMALITLESQENHSIQPSELSCALGSSRTNATRIADELEKRGWIE
                     RRESDNDRRCLHLQLTEKGHEFLREVLPPQHNCLHQLWSALSTTEKDQLEQITRKLLS
                     RLDQMEQDGVVLEAMS"
     gene            complement(6968..7303)
                     /gene="ygaH"
                     /locus_tag="ACHOSC_RS14445"
                     /old_locus_tag="pgaptmp_002889"
     CDS             complement(6968..7303)
                     /gene="ygaH"
                     /locus_tag="ACHOSC_RS14445"
                     /old_locus_tag="pgaptmp_002889"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-valine transporter subunit YgaH"
                     /protein_id="HGU4548793.1"
                     /translation="MSYEVLLLGLLVGVANYCFRYLPLRLRVGNARPTKRGAVGILLD
                     TIGIASICALLVVSTAPEVMHDTRRFVPTLVGFAVLGASFYKTRSIIIPTLLSALAYG
                     LAWKVMAII"
     gene            complement(7293..8030)
                     /gene="ygaZ"
                     /locus_tag="ACHOSC_RS14450"
                     /old_locus_tag="pgaptmp_002890"
     CDS             complement(7293..8030)
                     /gene="ygaZ"
                     /locus_tag="ACHOSC_RS14450"
                     /old_locus_tag="pgaptmp_002890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-valine exporter subunit YgaZ"
                     /protein_id="HGU4548794.1"
                     /translation="MESPTPQPAPGSATFMEGCKDSLPIVISYIPVAFAFGLNATRLG
                     FSPLESVFFSCIIYAGASQFVITAMLAAGSSLWVAALTVMAMDVRHVLYGPSLRSRII
                     QRLQKSKTALWAFGLTDEVFAAATAKLVRNNRRWSENWMIGIAFSSWSSWVFGTVIGA
                     FSGSGLLQGYPAVEAALGFMLPALFMSFLLASFQRKQSLCVTAALVGALAGVTLFSIP
                     VAILAGIVCGCLTALIQAFWQGAPDEL"
     gene            complement(8154..9338)
                     /locus_tag="ACHOSC_RS14455"
                     /old_locus_tag="pgaptmp_002891"
     CDS             complement(8154..9338)
                     /locus_tag="ACHOSC_RS14455"
                     /old_locus_tag="pgaptmp_002891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HGU4548795.1"
                     /translation="MTKPNHELSPALIVLMSIATGLAVASNYYAQPLLDTIARNFSLS
                     ASSAGFIVTAAQLGYAAGLLFLVPLGDMFERRRLIVSMTLLAAGGMLITASSQSLAMM
                     LLGTALTGLFSVVAQILVPLAATLASPDKRGKVVGTIMSGLLLGILLARTVAGLLANL
                     GGWRTVFWVASVLMALMALALWRGLPQMKSETHLNYPQLLGSVFSMFINDKILRTRAL
                     LGCLTFANFSILWTSMAFLLAAPPFNYSDGVIGLFGLAGAAGALGARPAGGFADKGKS
                     HHTTTFGLLLLLLSWLAIWFGHTSVLALIIGILVLDLTVQGVHITNQTVIYRIHPDAR
                     NRLTAGYMTSYFIGGAAGSLISASAWQHGGWAGVCLAGATIALVNLLVWWRGFHRQEA
                     AN"
     gene            complement(9530..10522)
                     /gene="proX"
                     /locus_tag="ACHOSC_RS14460"
                     /old_locus_tag="pgaptmp_002892"
     CDS             complement(9530..10522)
                     /gene="proX"
                     /locus_tag="ACHOSC_RS14460"
                     /old_locus_tag="pgaptmp_002892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine/L-proline ABC transporter
                     substrate-binding protein ProX"
                     /protein_id="HGU4548796.1"
                     /translation="MRHSVLFATAFATLISTQTFAADLPGKGITVNPVQSTITEETFQ
                     TLLVSRALEKLGYTVNKPSEVDYNVGYTSLASGDATFTAVNWTPLHDNMYEAAGGDKK
                     FYREGVFVNGAAQGYLIDKKTADQYKITNIAQLKDPKIAKLFDTNGDGKADLTGCNPG
                     WGCEGAINHQLAAYELTHTVTHNQGNYAAMMADTISRYKEGKPVFYYTWTPYWVSNEL
                     KPGKDVVWLQVPFSALPGDKNADTKLPNGANYGFPVSTMHIVANKAWAEKNPAAAKLF
                     AIMQLPVADINAQNAIMHDGKASEGDIQGHVDGWIKAHQQQFDGWVNEALAAQK"
     gene            complement(10580..11644)
                     /gene="proW"
                     /locus_tag="ACHOSC_RS14465"
                     /old_locus_tag="pgaptmp_002893"
     CDS             complement(10580..11644)
                     /gene="proW"
                     /locus_tag="ACHOSC_RS14465"
                     /old_locus_tag="pgaptmp_002893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine/L-proline ABC transporter
                     permease ProW"
                     /protein_id="HGU4548797.1"
                     /translation="MADQNNPWDTTPAADSAVQSADAWGTPATAPTDGGGADWLTSTP
                     APNVEHFNILDPFHKTLIPLDSWVTEGIDWVVTHFRPVFQGVRVPVDYILNGFQQLLL
                     GMPAPVAIIVFALIAWQISGVGMGVATLVSLIAIGAIGAWSQAMVTLALVLTALLFCI
                     VIGLPLGIWLARSPRAAKIIRPLLDAMQTTPAFVYLVPIVMLFGIGNVPGVVVTIIFA
                     LPPIIRLTILGINQVPADLIEASRSFGASPRQMLFKVQLPLAMPTIMAGVNQTLMLAL
                     SMVVIASMIAVGGLGQMVLRGIGRLDMGLATVGGVGIVILAIILDRLTQAVGRDSRSR
                     GNRRWYTTGPVGLLTRPFIK"
     gene            complement(11637..>12772)
                     /gene="proV"
                     /locus_tag="ACHOSC_RS14470"
                     /old_locus_tag="pgaptmp_002894"
     CDS             complement(11637..>12772)
                     /gene="proV"
                     /locus_tag="ACHOSC_RS14470"
                     /old_locus_tag="pgaptmp_002894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0031460 - glycine betaine transport
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="glycine betaine/L-proline ABC transporter
                     ATP-binding protein ProV"
                     /protein_id="HGU4548798.1"
                     /translation="YIEQGLSKEQILEKTGLSLGVKDASLAIEEGEIFVIMGLSGSGK
                     STMVRLLNRLIEPTRGQVLIDGVDIAKISDAELREVRRKKIAMVFQSFALMPHMTVLD
                     NTAFGMELAGINAEERREKALDALRQVGLENYAHSYPDELSGGMRQRVGLARALAINP
                     DILLMDEAFSALDPLIRTEMQDELVKLQAKHQRTIVFISHDLDEAMRIGDRIAIMQNG
                     EVVQVGTPDEILNNPANDYVRTFFRGVDISQVFSAKDIARRTPNGLIRKTPGFGPRSA
                     LKLLQDEDREYGYVIERGNKFVGAVSIDSLKTALTQQQGLDAALIDAPLAVDAQTPLS
                     ELLSHVGQAPCAVPVVDEDQQYVGIISKGMLLRALDREGVNNG"
BASE COUNT         3168 a         3297 c         3374 g         2933 t
ORIGIN      
        1 ctcccaaaaa tttccttcaa aaatatctca tttctaaata actactttat ccatttcctg
       61 aattttcttc tccttcgcca tcagcgacaa aatcaagcaa ttacgctaaa gtagcgtcat
      121 atttttcgac ttgcgagaag accatgtacg agcgttatgc aggtttaatt tttgatatgg
      181 atggcacaat cctggatacg gagcctacgc accgtaaagc gtggcgcgaa gtattagggc
      241 actacggtct tcagtacgat gttcaggcaa tgattgcgct taatggatcg cccacctggc
      301 gtattgctca ggcaattatt gagctgaatc aggccgatct cgacccgcat gcgttagcgc
      361 gtgaaaaaac agaagcagta agaagtatgc tgctggatag tgtcgagccg cttcctcttg
      421 ttgaagtggt gaaaagttgg catggtcgtc gcccaatggc tgtaggaacg gggagtgaaa
      481 gcgccatcgc tgaggcattg ctggcgcacc tgggattacg ccgttatttt gacgccgtcg
      541 tcgctgctga tcacgtcaaa taccataaac ccgcgccaga cacatttttg ttgtgcgcgc
      601 agcgtatggg cgtgcaaccg acgcagtgtg tggtctttga agatgccgat ttcggtattc
      661 aggcggcccg tgcagcaggc atggacgccg tggatgttcg cttgctgtga gtgaagcgtt
      721 atcgcttttc tcgttgtttg ccagtagttt tctcagcgct acactattac ccggcaactc
      781 cgaagtcgtg ctggtggcaa tgttgctttc cgggatcagt tatccctggg ttttagtttt
      841 aacagcaaca atgggtaata gccttggagg gttaactaac gttatccttg ggcgtttctt
      901 tccattgcgt aaaacatcgc gctggcaaga gaaagctacc ggctggctga aacgctatgg
      961 tgcagtcacg ctattattaa gctggatgcc cgtggttggc gatttactgt gtctgttagc
     1021 gggatggatg cgcatctcct gggggccagt aatctttttt ttgtgccttg gtaaagcgtt
     1081 acgctatgtt gcagttgcag cagcgaccgt tcagggcatg atgtggtggc actaattgta
     1141 ggcctgcaca tatggtcacc attacagtta tgctaattaa aacgattttg acaggcggga
     1201 ggtcaatttg atcccggacg tatcacaggc gctggcctgg ctggaaaaac atcctcaggc
     1261 gttaaagggg atacagcgtg ggctggagcg cgaaactttg cgtgttaatg ctgatggcac
     1321 actggcaaca acaggtcatc ctgaagcatt aggttccgca ctgacgcaca aatggattac
     1381 taccgatttt gcggaagcat tgctggaatt cattacacca gtggatggtg atattgaaca
     1441 tatgttgacc tttatgcgcg atctgcatcg ttatacggcg cgcaatatgg gcgatgagcg
     1501 gatgtggccg ttaagtatgc catgctacat cgcagaaggt caggacatcg aactggcaca
     1561 gtacggcact tctaacaccg gacgctttaa aacgctgtat cgtgaagggc tgaaaaatcg
     1621 ctacggcgcg ctgatgcaaa ccatttccgg cgtgcactac aatttctctt tgccaatggc
     1681 attctggcaa gcgaagtgcg gtgatatctc gggcgctgat gccaaagaga aaatttctgc
     1741 gggctatttc cgcgttatcc gcaattacta tcgtttcggt tgggtcattc cttatctgtt
     1801 tggtgcatct ccggcgattt gttcttcttt cctgcaagga aaaccaacgt cgctgccgtt
     1861 tgagaaaacc gagtgcggta tgtattacct gccgtatgcg acctctcttc gtttgagcga
     1921 tctcggctat accaataaat cgcaaagcaa tcttggtatt accttcaacg atctttacga
     1981 gtacgtagcg ggccttaaac aggcaatcaa aacgccatcg gaagagtacg cgaagattgg
     2041 tattgagaaa gacggtaaga ggctgcaaat caatagcaac gtgctgcaga ttgaaaacga
     2101 actgtacgcg ccgattcgtc caaaacgcgt tacccgcagc ggcgagtcac cttctgatgc
     2161 gctgttacgt ggcggcattg aatatattga agtgcgttcg ctggacatca acccgttctc
     2221 gccgattggt gtagatgaac agcaggtgcg attcctcgac ctgtttatgg tctggtgtgc
     2281 gctggctgat gcaccggaaa tgagcagtag cgaacttgcc tgtacgcgcg ttaactggaa
     2341 ccgggtgatc cttgaaggtc gcaaaccggg cctgacgctg ggtatcggct gcgaaaccgc
     2401 gcagttcccg ttactgcagg tgggtaaaga tctgttccgc gatctgaaac gcgtcgcgca
     2461 aacgctggat agcattaacg gcggcgaagc gtatcagaaa gtgtgtgatg aactggttgc
     2521 ctgcttcgat aatcccgatc tgactttctc tgcccgtatc ttaaggtcta tgattgatac
     2581 tggtattggc ggaacaggca aagcgtttgc agaagcctac cgtaatctgc tgcgtgaaga
     2641 gccgctggaa attctgcgcg aagaggattt tgtagccgag cgcgaggcat ctgaacgccg
     2701 tcagcaggaa atggaaaccg ctgataccga accgtttgcg gtgtggctgg aaaaacacgc
     2761 ctgacagaaa agaaaaaggc cactcgtgag tggccaaaat ttcatctctg aattcaggga
     2821 tgatgataac aaatgcgcgt ctttcatata ctcagactcg cctgggaaga aagagttcag
     2881 aaaattttaa aaaaaattac cggaggtggc taaatgccgt tgttagatag cttcacagtc
     2941 gatcataccc ggatggaagc gcctgcagtt cgggtggcga aaacaatgaa caccccgcat
     3001 ggcgacgcaa tcaccgtgtt cgatctgcgc ttctgcgtgc cgaacaaaga agtgatgcca
     3061 gaaagaggga tccataccct ggagcacctg tttgctggtt ttatgcgtaa ccatcttaac
     3121 ggtaagggtg tagagattat cgatatctcg ccaatgggct gccgcaccgg tttttatatg
     3181 agtctgattg gtacgccaga tgagcagcgt gttgctgatg cctggaaagc ggcaatggaa
     3241 gacgtgctga aagtgcagga tcagaatcag atcccggaac tgaacgtcta ccagtgtggc
     3301 acttaccaga tgcactcgtt gcaggaagcg caggatattg cgcgtagcat tctggaacgt
     3361 gacgtacgca tcaacagcaa cgaagaactg gcactgccga aagagaagtt gcaggaactg
     3421 cacatctagt cagtaaacta tcttcacaat taattgaaaa aagccagttc aaatgaactg
     3481 gcttagttgt acttagtgcg caccgcctcc gccgccacct gcgccaaatg gcggtttagc
     3541 aaaccacacc agccccagca gtacgaggaa tattccggct gacatccaga agatctcatt
     3601 ggcggaaata atcagcccct gattggtgat ctgctgggca atccagcctg atgcctgctg
     3661 ttgcgtcatc ccaagccctt ccagttgact gtacatcgcc tgggcattcg ggttgaacgg
     3721 gtttaccgac tcagtcaact gcgcatggtg catcgactcg cggttggtcc acatggtcgt
     3781 ggttatcgac gtgccgatag accccgccag cgttcgcgta aagttagaga ggctcgatgc
     3841 cgccgccagt cgttccggtg gcaaaccaga cagcgtaatg gtggtcagcg gcataaagaa
     3901 gcaggccacc gcaaacccct ggataaactg cggccaggcc gacgcgccaa aatccatacc
     3961 tggttcaaag gtataggcac gccagtagaa gcagacggca tacataataa agctgaaggt
     4021 taccagccgc cgcatatcca gtttatgcgc gaagcggccg ataatcggcg acaggatcac
     4081 cggaataatc cctaccggcg cagaggccaa acctgcccag gtcgccgtgt aaccgtagac
     4141 ctcctgcaac aactgcggca gcagaacaat agcgccgaag tagagcatat acgcgaggct
     4201 gatacacaag cagccgatgg tgaagttgcg cgacttaaac aacgacagat cgactatcgg
     4261 gttatcgtcg gtcagctccc agacaatcag gaagcagata gccaccaccg ccaccacggt
     4321 aaggatgata atttcctgtg atgaaaacca atccagctct ttaccgcggt caagcataat
     4381 ctgcaggctg ccgataccaa taaccagcag tgccagcccc acggcatcaa tccgccgccg
     4441 ttcggtgcgg gtttcacgtc cgcgcagagt ttgcagtgtc atcaacacca ccgccacgcc
     4501 aatcggcacg ttgatgaaga atatccagcc ccagtggtaa ttatcgctga tataaccgcc
     4561 gaggatcggg ccgcaaattg gcgcgacaat caccgtcatc gaccacaacg ccagcgcgat
     4621 cgagcgtttg gctggcgggt agttattcag caatagactt tgcgaaagcg ggatcaacgg
     4681 cccggcgaca atcccctgaa tcacgcggaa gaagatcagc atattcaggc tgctggagac
     4741 accacacgcc cacgacgcaa tagcaaaggc gatggtggac caaaggaaca gtttcacttc
     4801 cccgacgcgc tttgccagcc agccggtaag cgggatcgag atggcattcg ccaccccgaa
     4861 agaagtgatt acccacgttc cctggctgag cgatgagccc agattcccgg cgatagtggg
     4921 gatcgccacg ttagcaatgg tggagtccag cacctgcatg aatgtcgcca gtgacagcgc
     4981 aatcgtcata atgaccagtt gcgcgccttc cagcggtttt tgctgttgca tcacacgcac
     5041 ctctggatta gccagcgtta gcttttacga tatcgtcgat cagtttattg acaggtgcca
     5101 ggctgatttc acgcgcggta cttaccgcga ccggtgttga acgtacttta tttgccagta
     5161 cctgaccgtc acggttagtg gtattgacgc tcaccagcgt ggacaaacca atgcgcagcg
     5221 ggtattgctc cagctgtttc tggtccagtt cgatacgcac aggtagacgc tgaacgactt
     5281 tgatccagtt accggtcgca ttttgcgccg gaagcagtga gaatgcgcta cctgtgccca
     5341 tatccagacc aaccacttta ccggtgtatt tcacatcatc gccgtaaata tccgtggtga
     5401 tagtgaccgg ctgaccgata cgcatattgg caatctgcgt ctctttaaag ttggcatcca
     5461 cccacatatt ggtggctgga acgaccgcca tcagcggcgt cgttgggcta atttgcgccc
     5521 caggctgtac cgcgcggcgg gagacataac cggtcatcgg actgacaata cgggtacgct
     5581 ccagcgccag ccaggcgtta cgtacttcgg tggcagcctg ttgcacggca ggctgatctt
     5641 ccagtttagt ccccagaatc atcgcctgat tggcattgta ttgttgaata gcgacgtcca
     5701 gttgcgcctg ggcactggtg acggcgtcgc gggcgtgttg cagctcttcg cgaccaatca
     5761 ggttggcatt gcccagcggc acacggcggt tgtagtcgct ttgtgctttc gcgagggcga
     5821 ttttttgcac ctcaatattc gcctgcaact gtttgctgtt aatcatcagc tggtgggttt
     5881 ggcgaacgct ggaagccagt gcagttttgg ctttttcaaa cgcctggcga gcatctgtcg
     5941 ggtcgagagt gaccagaaca tcgccttctt ttacaaaatc ggtgttatcg gcccagactt
     6001 tcgtcacgct gccagacacc tgagacataa tttgaatctg attccctgcc acgtatgcgt
     6061 catcggtttc ttcgaaatga cgcagtacca aaaaccaata aatccctatc gctacggcaa
     6121 taattataaa gagcaaggtg agaaggagga gcagacgctt acgtttgccg ctcttcttta
     6181 ccggttgctg cggggtttga gtctccgcat ttgcgctcat attgttctcc acgatcttct
     6241 taactcatcg gctgagccga gctgcttgcc aaaaaggcca gcatgttgcg atgctggccg
     6301 gtcatttttt cttttataaa tctggatttt tgagcgagat gacgcgttag ctcatcgctt
     6361 cgagaaccac accgtcttgt tccatctggt cgagacggga gagcaatttg cgggtgattt
     6421 gctcgagctg atctttttct gttgtgctga gcgcggacca gagttgatgc aggcagttat
     6481 gctgcggtgg taaaacctcg cgcaaaaact cgtgaccttt ttccgttaat tgcagatgca
     6541 ggcagcggcg atcgttatcg ctttcacgac gttcgatcca accgcgtttt tccagttcat
     6601 cggcaatacg cgtcgcgttg gtacgggatg atccaagagc acaacttaat tcagaaggct
     6661 gaatactgtg gttttcctga gactccagcg taatcaacgc cataaacaac gtctcgttaa
     6721 tcccctgagc cttcagcatt ttattgcggt tctccagcag cttgctttgc atgtgcatgc
     6781 aaagacgagt cagaaggatc tcctgataag gaaaatcttc gtggcggctg gcgcgaaatt
     6841 ttagcatttg ttcaatgggc gtaaacgaac tatccatttg ggtatgacct cattaatttc
     6901 agccgatata gtaacgacag tgacaaataa agtaaatgta ttgttttaga aaaatgattc
     6961 ttgtgggtta tataatcgcc atcactttcc aggcgagccc ataggccagc gcactaagca
     7021 gtgttgggat gataatgctg cgtgttttat agaaactggc acccagtacc gcgaagccga
     7081 ccagcgtggg cacgaaacgg cgtgtatcgt gcatcacttc tggtgcggta gagacaacca
     7141 gcagagcgca tatcgaggcg atgccaatgg tgtcgagcaa aatacctacc gcgccacgtt
     7201 tggttgggcg ggcattaccc acacgcaggc gcagcggcaa atagcggaag caataattcg
     7261 ccacgccaac cagtaaccca agcagcagaa cctcatagct catcgggcgc tccttgccag
     7321 aatgcctgga ttaacgcagt gaggcagcca cagacaatgc ctgccagaat ggcgacggga
     7381 atagaaaata gcgttacgcc tgcaagggca ccaactaacg ctgcggtaac gcaaagagat
     7441 tgtttgcgct ggaaagaggc gagcaggaaa ctcataaaga gtgccggaag cataaaacct
     7501 aatgcagctt caacggcggg ataaccttgc agcaagccgc tgccggagaa tgcccctatt
     7561 accgtaccaa atacccacga tgaccatgaa ctgaaggcaa tgccgatcat ccagttctcg
     7621 ctccagcggc gattattgcg taccagtttt gcggttgcgg cggcaaaaac ctcatccgtc
     7681 aggccaaatg cccacagggc ggttttcgat ttttgcagac gctgaataat acggctacgc
     7741 agtgacgggc catacaacac atggcgaaca tccattgcca tgacggtcag tgcagcaacc
     7801 cacaaactac tcccggctgc cagcatcgcg gtaatgacga actggctcgc gcctgcataa
     7861 atgatgcagg agaaaaaaac gctttcgaga ggagagaatc ccagacgggt cgcattcaga
     7921 ccgaacgcaa aggccaccgg aatataacta ataacaatcg gtaaactgtc tttgcatcct
     7981 tccatgaagg tcgccgaacc aggagcaggc tgtggagtag ggctttccat agagtgtacg
     8041 cttaacgatt gttaatatta taaatagact gaatgaatat cttaacctta tcagactgat
     8101 gggcttctta acacccttat aagtgtaaag ccacgaaaac ggttgctgat tgcttaattt
     8161 gcggcttcct gacgatgaaa acctcgccac cagaccagta agttaaccag ggcaatcgtc
     8221 gcgccagcca gacaaacgcc agcccaaccg ccatgttgcc aggctgaggc tgaaattagc
     8281 gaaccggcgg caccgccaat aaagtagctg gtcatgtaac ctgcggtcag gcgattacgc
     8341 gcatcagggt gtattcgata aattaccgtc tggttagtga tatgcacgcc ctgtacggtg
     8401 agatccagca ccaggattcc gataatcaac gccagtacgg aagtgtgacc aaaccagatc
     8461 gccagccatg aaagtaatag cagcagcaga ccgaaagttg tggtgtggtg cgatttaccc
     8521 ttatcggcaa aaccgcccgc cggacgagcg cccaacgcgc cggcagctcc cgcaagtcca
     8581 aacagaccaa tgacaccatc gctgtagtta aaaggtggag cggcaagcaa aaaggccatt
     8641 gaggtccaga gaatgctgaa gttggcaaag gtcaggcagc caagcaatgc gcgggtgcgc
     8701 aggatcttat cgttgataaa catgctgaaa acggaaccca acaactgtgg gtagttgagg
     8761 tgggtttctg atttcatttg tggcagacca cgccataatg ctagcgccat cagagccatt
     8821 aacaccgagg caacccaaaa gacggtgcgc cagccgccga ggtttgccag taacccggca
     8881 acggttcgtg ccagcaatat ccccaacagc agaccgctca taatggtgcc aaccactttg
     8941 ccgcgtttat ccggtgaagc cagcgtcgct gccagcggaa ccagaatttg tgccacgaca
     9001 gagaataaac cggttaatgc cgtaccgagg agcatcatcg ccagcgactg actgctggcg
     9061 gtaatcaaca taccgcccgc tgccagtaag gtcatcgaga caatcaggcg gcggcgttca
     9121 aacatatcac cgaggggaac aagaaacagc agacctgctg catagcccaa ttgcgcggcg
     9181 gtaacaataa agcctgccga actggcggaa agggaaaagt tacgcgcgat ggtgtcgagt
     9241 agcggctggg cgtaatagtt actggcgacc gccagaccgg tggcgataga catcagcacg
     9301 atcagcgccg ggctaagctc atgattaggt ttagtcatta tttttgttca tcaggtgatg
     9361 attaagctgg aaatcaataa tagcggatgt tgatgaatat gggatgaaag atagcttgtt
     9421 gaaatgtgcg atgtggtttg taggcatgat aagacgcgtc agcgtcgcat caggcattgt
     9481 gtacagagcg gcggatgagg caaaaacgcc ttatccgccc gaataaaaat tacttctgcg
     9541 ctgccagcgc ctcattcacc cagccatcga actgctgctg gtgggctttg atccagccat
     9601 caacatggcc ctgaatatcg ccttctgagg ctttgccgtc atgcataatg gcgttctggg
     9661 cgttaatatc tgccactggc aactgcataa tggcaaacag tttcgctgct gccgggtttt
     9721 tctcggccca ggctttgttg gcaacgatat gcatggtgct gaccgggaag ccataattcg
     9781 caccattcgg cagtttggta tcggcgtttt tatcgcccgg cagtgcggag aacggcacct
     9841 gcaaccagac cacatctttc cctggcttca gctcattact cacccagtac ggcgtccagg
     9901 tgtagtaaaa caccggtttg ccctctttgt agcgactgat ggtgtcggcc atcatcgccg
     9961 cgtagttccc ctgattatgc gtcacggtat gggtcagttc atacgcggca agctggtggt
    10021 tgatcgcacc ttcgcagccc cagccagggt tacaaccggt taaatccgct tttccgtcgc
    10081 cgttggtatc gaacagtttg gcgatcttcg gatctttcag ttgtgcgatg ttggtgattt
    10141 tgtactggtc ggcggttttc ttatcgatca ggtaaccctg tgccgcgccg ttaacaaata
    10201 ccccttcacg ataaaatttc ttatcgccac cggcagcttc gtacatgttg tcatgcagtg
    10261 gcgtccagtt cacggcggtg aaggttgcat cgccggaagc aagcgaggtg tagccaacgt
    10321 tgtaatccac ttcgctgggt ttgttgacgg tataacctaa tttctccagc gcacgactga
    10381 ccagcagcgt ctggaaggtt tcttcagtga tggtgctctg aactggatta acagtaatgc
    10441 ctttgcccgg cagatcggca gcaaaagttt gtgtagagat aagcgtggca aacgctgtcg
    10501 caaaaagtac gctatgtcgc attgttattc ctttttttgg cagggtgata atgcccggcg
    10561 tcaccgggca agtgcagagt tacttaatga atgggcgggt cagcagacca acagggccag
    10621 tggtgtacca gcgacggttg ccgcgactgc gtgagtcgcg cccaacggcc tgcgtcaggc
    10681 gatcgaggat aatggcgagg atcacaatcc cgacgccgcc aacggtggca agccccatat
    10741 ccagacgacc gataccgcga agtaccatct gacccagccc gccgacggca atcatcgagg
    10801 caatgaccac catagaaagg gccagcatca gcgtctggtt aacgcccgcc ataatggtcg
    10861 gcatcgccag cggtaactga actttgaaca gcatctggcg cgggctggca ccgaatgagc
    10921 gcgaggcttc aatcagatcc gccggaacct ggttaatccc cagaatggtc agacggataa
    10981 tcggcggcag agcaaagatg atcgtcacca ccacgcccgg cacgttaccg ataccaaata
    11041 gcatgacgat tggcaccaga taaacaaacg ctggcgtggt ctgcatggca tcaagcagtg
    11101 gacgaataat tttcgccgct cgcggacttc tcgccagcca gatccccagc ggcaaaccaa
    11161 tgacgataca gaacagcagg gcggttaaca ccagcgccag ggtaaccatg gcctgcgacc
    11221 aggcaccgat tgcgccgatg gcaatcagcg aaaccagcgt cgccacgccc attccgaccc
    11281 cggaaatctg ccaggcgatg agagcgaaaa cgataatcgc caccggtgcg ggcataccca
    11341 gcagcaattg ctggaaaccg ttgagaatgt aatcaaccgg aacgcgcacg ccctggaaga
    11401 cgggacggaa atgggtaaca acccagtcga tcccttcagt gacccaactg tcgagcggga
    11461 tcagcgtttt atggaacgga tcgagaatat taaaatgctc gacgtttggc gcaggcgtac
    11521 tggtcagcca gtcagcacca ccgccgtcag tcggtgcagt cgccggtgta ccccaggcgt
    11581 ctgcggattg cacggcactg tccgccgctg gcgtggtatc ccacggatta ttttgatcag
    11641 ccattattta ccccctcacg atctaaagcg cgcagcagca ttcctttcga aatgatgccg
    11701 acatactgtt ggtcctcgtc gaccacgggc accgcacagg gtgcctgtcc gacatgagag
    11761 agcaactcgc taagaggcgt ttgtgcatcg actgctaacg gcgcatcaat cagcgccgca
    11821 tcaagacctt gctgctgcgt taacgcggtt ttaagcgaat cgatggaaac tgcgccgaca
    11881 aacttattac cgcgttcgat aacgtagcca tattcgcgat cttcatcctg caataatttc
    11941 agtgccgaac gtgggccgaa gccaggggtt ttacgaatta agccattcgg tgtccggcgg
    12001 gcaatatctt tcgcactgaa tacctgacta atatcaacgc cacggaagaa ggtacggaca
    12061 taatcattcg ccggattatt gagaatttca tccggtgtgc cgacctgtac cacttcacca
    12121 ttttgcataa tggcaattcg gtcgccaata cgcatagcct catcgagatc gtgggaaata
    12181 aagacaatgg tgcgctgatg tttcgcctgt aattttacca gctcatcctg catctcggtg
    12241 cgaattaatg gatcgagcgc cgagaaggct tcgtccatta ataatatatc cggattaatc
    12301 gctaacgcgc gggctaatcc cacacgttga cgcatcccgc cagagagttc atccgggtag
    12361 ctgtgggcat aattttccag cccgacctga cgcagtgcat caagggcttt ttcccggcgt
    12421 tcttcggcat taattccggc caattccata ccgaacgcag tattgtccag cacggtcata
    12481 tgcggcatta aggcaaagga ctggaagacc atcgcaatct tttttctgcg cacctcacgg
    12541 agttcggcat cggatatttt ggcaatatcc acaccatcaa tcagcacttg cccgcgggtg
    12601 ggttcaatca ggcgattgag aaggcgtacc attgtggatt tacccgagcc ggataatccc
    12661 atgatgacaa atatctcgcc ttcttcaatg gccagactgg cgtcttttac gccaagcgat
    12721 agcccagttt tttccagaat ttgttctttc gaaagtcctt gttcgatata tt
//
LOCUS       DAZKHF020000127        12653 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2201S31241
            SAMN43267800-rid25106413.denovo.128, whole genome shotgun sequence.
ACCESSION   DAZKHF020000127 DAZKHF020000000
VERSION     DAZKHF020000127.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267800
            Sequence Read Archive: SRR30307263
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 12653)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12653)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 12653)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350581.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350581.2
            Genome Coverage       :: 151x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:21:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,652
            CDSs (total)                      :: 5,557
            Genes (coding)                    :: 5,320
            CDSs (with protein)               :: 5,320
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 237
            CDSs (without protein)            :: 237
            Pseudo Genes (ambiguous residues) :: 0 of 237
            Pseudo Genes (frameshifted)       :: 102 of 237
            Pseudo Genes (incomplete)         :: 130 of 237
            Pseudo Genes (internal stop)      :: 52 of 237
            Pseudo Genes (multiple problems)  :: 41 of 237
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12653
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267800-rid25106413.denovo.128"
                     /serotype="O 26:H11"
                     /isolate="2201S31241"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-01"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            complement(15..1373)
                     /gene="plaP"
                     /locus_tag="ACHOSC_RS14475"
                     /old_locus_tag="pgaptmp_002895"
     CDS             complement(15..1373)
                     /gene="plaP"
                     /locus_tag="ACHOSC_RS14475"
                     /old_locus_tag="pgaptmp_002895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416518.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putrescine/proton symporter PlaP"
                     /protein_id="HGU4548799.1"
                     /translation="MSHNVTPNTSRVELRKTLTLVPVVMMGLAYMQPMTLFDTFGIVS
                     GLTDGHVPTAYAFALIAILFTALSYGKLVRRYPSAGSAYTYAQKSISPTVGFMVGWSS
                     LLDYLFAPMINILLAKIYFEALVPSIPSWMFVVALVAFMTAFNLRSLKSVANFNTVIV
                     VLQVVLIAVILGMVVYGVFEGEGAGTLASTRPFWSGDAHVIPMITGATILCFSFTGFD
                     GISNLSEETKDAERVIPRAIFLTALIGGMIFIFATYFLQLYFPDISRFKDPDASQPEI
                     MLYVAGKAFQVGALIFSTITVLASGMAAHAGVARLMYVMGRDGVFPKSFFGYVHPKWR
                     TPAMNIILVGAIALLAINFDLVMATALINFGALVAFTFVNLSVISQFWIREKRNKTLK
                     DHFQYLFLPMCGALTVGALWVNLEESSMVLGLIWAAIGLIYLACVTKSFRNPVPQYED
                     VA"
     gene            complement(1363..1425)
                     /gene="yoeI"
                     /locus_tag="ACHOSC_RS14480"
                     /old_locus_tag="pgaptmp_002896"
     CDS             complement(1363..1425)
                     /gene="yoeI"
                     /locus_tag="ACHOSC_RS14480"
                     /old_locus_tag="pgaptmp_002896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein YoeI"
                     /protein_id="HGU4548800.1"
                     /translation="MGQFFAYATVITVKENDHVA"
     gene            complement(1640..2569)
                     /gene="yeeY"
                     /locus_tag="ACHOSC_RS14485"
                     /old_locus_tag="pgaptmp_002897"
     CDS             complement(1640..2569)
                     /gene="yeeY"
                     /locus_tag="ACHOSC_RS14485"
                     /old_locus_tag="pgaptmp_002897"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416519.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR substrate-binding domain-containing
                     protein"
                     /protein_id="HGU4548801.1"
                     /translation="MKPLLDVLMILDALEKEGSFAAASAKLYKTPSALSYTVHKLESD
                     LNIQLLDRSGHRAKFTRTGKMLLEKGREVLHTVRELEKQAIKLHEGWENELVIGVDDT
                     FPFSLLAPLIEAFYQHHSVTRLKFINGVLGGSWDALTQGRADIIVGAMHEPPSSSEFG
                     FSRLGDLEQVFAVAPHHPLALEEEPLNRRIIKRYRAIVVGDTAQAGASTASQLLDEQE
                     AITVFDFKTKLELQISGLGCGYLPRYLAQRFLDSGALIEKKVVAQTLFEPVWIGWNEQ
                     TAGLASGWWRDEILANSAIAGVYAKSDDGKSAI"
     gene            complement(2615..3439)
                     /gene="yeeZ"
                     /locus_tag="ACHOSC_RS14490"
                     /old_locus_tag="pgaptmp_002898"
     CDS             complement(2615..3439)
                     /gene="yeeZ"
                     /locus_tag="ACHOSC_RS14490"
                     /old_locus_tag="pgaptmp_002898"
                     /EC_number="1.1.1.290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HGU4548802.1"
                     /translation="MKKVAIVGLGWLGMPLAMSLSARGWQVTGSKTTQDGVEAARMSG
                     IDSYLLRMEPELVCDSDDLDALMDADALVITLPARRSGPGDEFYLQAVQELVDSALAH
                     RIPRIIFTSSTSVYGDAQGTVKETTPRNPVTNSGRVLEELEDWLHNLPGTSVDILRLA
                     GLVGPGRHPGRFFAGKTAPDGEHGVNLVHLEDVIGAITLLLQAPKGGHIYNICAPAHP
                     ARNVFYPQMARLLGLEPPQFRNSLDSGKGKIIDGSRICNELGFEYQYPDPLVMPLE"
     gene            complement(3522..3776)
                     /gene="yoeB"
                     /locus_tag="ACHOSC_RS14495"
                     /old_locus_tag="pgaptmp_002899"
     CDS             complement(3522..3776)
                     /gene="yoeB"
                     /locus_tag="ACHOSC_RS14495"
                     /old_locus_tag="pgaptmp_002899"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system mRNA interferase
                     toxin YoeB"
                     /protein_id="HGU4548803.1"
                     /translation="MKLIWSEESWDDYLYWQETDKRIVKKINELIKDTRRTPFEGKGK
                     PEPLKHNLSGFWSRRITEEHRLVYAVTDDSLLIAACRYHY"
     gene            complement(3773..4024)
                     /gene="yefM"
                     /locus_tag="ACHOSC_RS14500"
                     /old_locus_tag="pgaptmp_002900"
     CDS             complement(3773..4024)
                     /gene="yefM"
                     /locus_tag="ACHOSC_RS14500"
                     /old_locus_tag="pgaptmp_002900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416521.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YoeB-YefM toxin-antitoxin system antitoxin YefM"
                     /protein_id="HGU4548804.1"
                     /translation="MRTISYSEARQNLSATMMKAVEDHAPILITRQNGEACVLMSLEE
                     YNSLEETAYLLRSPANARRLMDSIDSLKSGKGTEKDIIE"
     gene            4307..4354
                     /gene="hisL"
                     /locus_tag="ACHOSC_RS14505"
                     /old_locus_tag="pgaptmp_002901"
     CDS             4307..4354
                     /gene="hisL"
                     /locus_tag="ACHOSC_RS14505"
                     /old_locus_tag="pgaptmp_002901"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000105 - L-histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="his operon leader peptide"
                     /protein_id="HGU4548805.1"
                     /translation="MTRVQFKHHHHHHPD"
     misc_feature    4330..4453
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00514"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="His leader region; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /db_xref="RFAM:RF00514"
     gene            4500..5399
                     /gene="hisG"
                     /locus_tag="ACHOSC_RS14510"
                     /old_locus_tag="pgaptmp_002902"
     CDS             4500..5399
                     /gene="hisG"
                     /locus_tag="ACHOSC_RS14510"
                     /old_locus_tag="pgaptmp_002902"
                     /EC_number="2.4.2.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005128078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003879 - ATP phosphoribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0000105 - L-histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP phosphoribosyltransferase"
                     /protein_id="HGU4548806.1"
                     /translation="MTDNTRLRIAMQKSGRLSDDSRELLARCGIKINLHTQRLIAMAE
                     NMPIDILRVRDDDIPGLVMDGVVDLGIIGENVLEEELLNRRAQGEDPRYFTLRRLDFG
                     GCRLSLATPVDEAWDGPLSLNGKRIATSYPHLLKRYLDQKGISFKSCLLNGSVEVAPR
                     AGLADAICDLVSTGATLEANGLREVEVIYRSKACLIQRDGEMEESKQQLIDKLLTRIQ
                     GVIQARESKYIMMHAPTERLDEVIALLPGAERPTILPLAGDQQRVAMHMVSSETLFWE
                     TMEKLKALGASSILVLPIEKMME"
     gene            5405..6709
                     /gene="hisD"
                     /locus_tag="ACHOSC_RS14515"
                     /old_locus_tag="pgaptmp_002903"
     CDS             5405..6709
                     /gene="hisD"
                     /locus_tag="ACHOSC_RS14515"
                     /old_locus_tag="pgaptmp_002903"
                     /EC_number="1.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004399 - histidinol dehydrogenase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0000105 - L-histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol dehydrogenase"
                     /protein_id="HGU4548807.1"
                     /translation="MSFNTIIDWNSCTAEQQRQLLMRPAISASESITRTVNDILDNVK
                     ARGDDALREYSAKFDKTTVTALKVSAEEIAAASERLSDELKQAMAVAVKNIETFHTAQ
                     KLPPVDVETQPGVRCQQVTRPVASVGLYIPGGSAPLFSTVLMLATPARIAGCKKVVLC
                     SPPPIADEILYAAQLCGVQDVFNVGGAQAIAALAFGTESVPKVDKIFGPGNAFVTEAK
                     RQVSQRQDGAAIDMPAGPSEVLVIADSGATPDFVASDLLSQAEHGPDSQVILLTPAAD
                     MARRVAEAVERQLAELPRAETARQALNASRLIVTNDLAQCVEISNQYGPEHLIIQTRN
                     ARDLVDSITSAGSVFLGDWSPESAGDYASGTNHVLPTYGYTATCSSLGLADFQKRMTV
                     QELSKEGFSALASTIETLAAAERLTAHKNAVTLRVNALKEQA"
     gene            6706..7776
                     /gene="hisC"
                     /locus_tag="ACHOSC_RS14520"
                     /old_locus_tag="pgaptmp_002904"
     CDS             6706..7776
                     /gene="hisC"
                     /locus_tag="ACHOSC_RS14520"
                     /old_locus_tag="pgaptmp_002904"
                     /EC_number="2.6.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004400 - histidinol-phosphate
                     transaminase activity [Evidence IEA];
                     GO_process: GO:0000105 - L-histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol-phosphate transaminase"
                     /protein_id="HGU4548808.1"
                     /translation="MSTVTITDLARENVRNLTPYQSARRLGGNGDVWLNANEYPAAVE
                     FQLTQQTLNRYPECQPKAVIENYAQYAGVKPEQVLVSRGADEGIELLIRAFCEPGKDA
                     ILYCPPTYGMYSVSAETIGVECRTVPTQDNWQLDLQGISDKLDGVKVVYVCSPNNPTG
                     QLINPQDFRTLLELTRGKAIVVADEAYIEFCPQASLAGWLAEYPHLAILRTLSKAFAL
                     AGLRCGFTLANEEVINLLMKVIAPYPLSTPVADIAAQALSPQGIVAMRERVAQIIAER
                     EYLIAALKEIPCVEQVFDSETNYILARFKASSAVFKSLWDQGIILRDQNKQPSLSGCL
                     RITVGTREESQRVIDALRAEQV"
     gene            7776..8843
                     /gene="hisB"
                     /locus_tag="ACHOSC_RS14525"
                     /old_locus_tag="pgaptmp_002905"
     CDS             7776..8843
                     /gene="hisB"
                     /locus_tag="ACHOSC_RS14525"
                     /old_locus_tag="pgaptmp_002905"
                     /EC_number="3.1.3.15"
                     /EC_number="4.2.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707917.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004401 - histidinol-phosphatase activity
                     [Evidence IEA];
                     GO_function: GO:0004424 - imidazoleglycerol-phosphate
                     dehydratase activity [Evidence IEA];
                     GO_process: GO:0000105 - L-histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     histidinol-phosphatase/imidazoleglycerol-phosphate
                     dehydratase HisB"
                     /protein_id="HGU4548809.1"
                     /translation="MSQKYLFIDRDGTLISEPPSDFQVDRFDKLAFEPGVIPELLKLQ
                     KAGYKLVMITNQDGLGTQSFPQADFDGPHNLMMQIFTSQGVQFDEVLICPHLPADECD
                     CRKPKVKLVERYLAEQAMDRANSYVIGDRATDIQLAENMGINGLRYDRETLNWPMIGE
                     QLTKRDRYAHVVRNTKETQIDVQVWLDREGDSKINTGVGFFDHMLDQIATHGGFRMEI
                     NVKGDLYIDDHHTVEDTGLALGEALKIALGDKRGICRFGFVLPMDECLARCALDISGR
                     PHLEYKAEFTYQRVGDLSTEMIEHFFRSLSYTMGVTLHLKTKGKNDHHRVESLFKAFG
                     RTLRQAIRVEGDTLPSSKGVL"
     gene            8843..9433
                     /gene="hisH"
                     /locus_tag="ACHOSC_RS14530"
                     /old_locus_tag="pgaptmp_002906"
     CDS             8843..9433
                     /gene="hisH"
                     /locus_tag="ACHOSC_RS14530"
                     /old_locus_tag="pgaptmp_002906"
                     /EC_number="4.3.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005128083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000107 - imidazoleglycerol-phosphate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0000105 - L-histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="imidazole glycerol phosphate synthase subunit
                     HisH"
                     /protein_id="HGU4548810.1"
                     /translation="MNVVILDTGCANLNSVKSAIARHGYEPKVSRDPDVVLLADKLFL
                     PGVGTAQAAMDQVRERELFDLIKACTQPVLGICLGMQLLGRRSEESNGVDLLGIIDED
                     VPKMTDFALPLPHMGWNRVYPQAGNRLFQGIEDGAYFYFVHSYAMPVNPWTIAQCNYG
                     EPFTAAVQKDNFYGVQFHPERSGAAGAKLLKNFLEM"
     gene            9433..10170
                     /gene="hisA"
                     /locus_tag="ACHOSC_RS14535"
                     /old_locus_tag="pgaptmp_002907"
     CDS             9433..10170
                     /gene="hisA"
                     /locus_tag="ACHOSC_RS14535"
                     /old_locus_tag="pgaptmp_002907"
                     /EC_number="5.3.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416528.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003949 -
                     1-(5-phosphoribosyl)-5-[(5-
                     phosphoribosylamino)methylideneamino]imidazole-4-
                     carboxamide isomerase activity [Evidence IEA];
                     GO_process: GO:0000105 - L-histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-(5-phosphoribosyl)-5-[(5-
                     phosphoribosylamino)methylideneamino]imidazole-4-
                     carboxamide isomerase"
                     /protein_id="HGU4548811.1"
                     /translation="MIIPALDLIDGTVVRLHQGDYGKQRDYGNDPLPRLQDYAAQGAE
                     LLHLVDLTGAKDPAKRQIPLIKTLVAGVNVPVQVGGGVRSEEDVAALLEAGVARVVVG
                     STAVKSPEMVKGWFERFGADALVLALDVRIDEQGNKQVAVSGWQENSGVSLEQLVESY
                     LPVGLKHVLCTDISRDGTLAGSNVSLYEEVCARYPQVAFQSSGGIGDIDDVAALRGTG
                     VRGVIVGRALLEGKFTVKEAIACWQNA"
     gene            10152..10928
                     /gene="hisF"
                     /locus_tag="ACHOSC_RS14540"
                     /old_locus_tag="pgaptmp_002908"
     CDS             10152..10928
                     /gene="hisF"
                     /locus_tag="ACHOSC_RS14540"
                     /old_locus_tag="pgaptmp_002908"
                     /EC_number="4.3.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000107 - imidazoleglycerol-phosphate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0000105 - L-histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="imidazole glycerol phosphate synthase subunit
                     HisF"
                     /protein_id="HGU4548812.1"
                     /translation="MLAKRIIPCLDVRDGQVVKGVQFRNHEIIGDIVPLAKRYAEEGA
                     DELVFYDITASSDGRVVDKSWVSRVAEVIDIPFCVAGGIKSLEDAAKILSFGADKISI
                     NSPALADPTLITRLADRFGVQCIVVGIDTWYDAETGKYHVNQYTGDESRTRVTQWETL
                     DWVQEVQKRGAGEIVLNMMNQDGVRNGYDLEQLKKVREVCRVPLIASGGAGTMEHFLE
                     AFRDADVDGALAASVFHKQIINIGELKAYLATQGVEIRIC"
     gene            10922..11533
                     /gene="hisIE"
                     /locus_tag="ACHOSC_RS14545"
                     /old_locus_tag="pgaptmp_002909"
     CDS             10922..11533
                     /gene="hisIE"
                     /locus_tag="ACHOSC_RS14545"
                     /old_locus_tag="pgaptmp_002909"
                     /EC_number="3.5.4.19"
                     /EC_number="3.6.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004635 - phosphoribosyl-AMP
                     cyclohydrolase activity [Evidence IEA];
                     GO_function: GO:0004636 - phosphoribosyl-ATP diphosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0000105 - L-histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional phosphoribosyl-AMP
                     cyclohydrolase/phosphoribosyl-ATP diphosphatase HisIE"
                     /protein_id="HGU4548813.1"
                     /translation="MLTEQQRRELDWEKTDGLMPVIVQHAVSGEVLMLGYMNPEALDK
                     TIESGKVTFFSRTKQRLWTKGETSGNFLNVVNIAPDCDNDTLLVLANPIGPTCHKGTS
                     SCFGDTAHQWLFLYQLEQLLAERKSADPETSYTAKLYASGTKRIAQKVGEEGVETALA
                     ATVHDRFELTNEASDLMYHLLVLLQDQDLDLTTVIENLRKRHQ"
     gene            complement(11630..12607)
                     /gene="wzzB"
                     /locus_tag="ACHOSC_RS14550"
                     /old_locus_tag="pgaptmp_002910"
     CDS             complement(11630..12607)
                     /gene="wzzB"
                     /locus_tag="ACHOSC_RS14550"
                     /old_locus_tag="pgaptmp_002910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310855.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS O-antigen chain length determinant protein
                     WzzB"
                     /protein_id="HGU4548814.1"
                     /translation="MRVENNNVSGQNHDPEQIDLIDLLVQLWRGKMTIIISVIVAIAL
                     AIGYLAVAKEKWTSTAIVTQPDVGQIAGYTNAMNVIYGSAVPKVSDIQASLIGRYSTA
                     FSALAETLDNQEEAEKLTIEPTVKNQSLPLAVSYVGETPEGAQKQLAKYIQQVDDQVN
                     EELGKDLKDNIALRMKNLQDSLKTQEVVAQEQKDLRIRQIQEALQYANQAQVTKPQVQ
                     QTEDVTQDTLFLLGSEALESMIKHEATRPLVFSPNYYQTRQNLLDIESLKVDDLDIHA
                     YRYVMKPTLPIRRDSPKKAITLILAVLLGGMVGAGIVLGRNALRNYNAK"
BASE COUNT         3138 a         3331 c         3273 g         2911 t
ORIGIN      
        1 attagaaaag atgattatgc tacgtcttcg tactgcggaa ccggattacg gaaactcttg
       61 gttacgcaag ccaggtaaat cagaccgata gcagcccaga tcagacccag aaccattgag
      121 ctttcttcca ggttaaccca cagcgcacca acggtcagcg caccacacat cggcaggaac
      181 agatactgga agtgatcttt cagcgtcttg ttacgcttct cacggatcca gaactgcgaa
      241 ataaccgaca ggttaacgaa ggtgaacgcc accagcgcac caaagttaat cagcgccgta
      301 gccattacca ggtcgaagtt gattgccagc aaggcaatcg cgccaaccag gatgatgttc
      361 attgccggag tacgccattt cgggtgtaca taaccgaaga agcttttcgg gaacacaccg
      421 tcacgcccca ttacgtacat cagacgcgct acgcctgcat gcgccgccat accggacgcc
      481 agtacggtaa tggtggagaa gatcagcgcg ccaacctgga acgctttacc cgcaacgtac
      541 agcatgattt caggctgtga cgcatccgga tctttaaagc gagagatatc cgggaagtac
      601 agctgcagga agtaagttgc aaagatgaag atcatgccgc caatcagtgc ggtcaggaaa
      661 atcgcacgcg ggatcacgcg ctctgcatct ttggtttctt ccgacaggtt gctgatgccg
      721 tcaaagccgg taaaggagaa gcacaggatt gtcgccccgg taatcatcgg gataacatgc
      781 gcatcgccag accagaacgg acgagtgctc gccagcgtac ccgcgccttc accttcaaat
      841 acgccataaa caaccatgcc cagaatcacc gcgatcagca ctacctgcaa cacgacgatt
      901 acagtgttga agttcgctac ggatttcaga ctacgcaggt taaaggcggt cataaaggcc
      961 accagcgcca ccacaaacat ccacgatggg atggaaggca ccagagcttc aaaatagatt
     1021 ttcgccagca gaatgttgat catcggcgcg aacagatagt cgagcagaga agaccaaccc
     1081 accataaagc cgacagtcgg gctaatggat ttctgggcgt aagtgtatgc agagccagca
     1141 gaaggatagc ggcgaaccag cttcccgtag ctcagagccg taaacaggat cgcaatcaat
     1201 gcgaacgcat aggctgtcgg cacatgacca tccgtgaggc ctgaaacgat accaaatgta
     1261 tcaaacagcg tcatcggctg catataggca agacccatca ttacaaccgg aactaacgta
     1321 agcgttttac gcaattccac gcgagaggtg tttggagtaa cgttatgcga catggtcatt
     1381 ctcctttacg gtgataaccg tcgcgtaagc aaaaaattgc cccatttttt tggattcctc
     1441 agcgacaaca actgtcgatt tttagtaaat atctatccgg tacgaagccc ggcctcttgg
     1501 tatgaatgat tggtttgaag caaaaaataa ccgacgctaa ttaaacgtcg gtttttagtc
     1561 attttttgac agcggcgcat tgtgcctaaa tgggggggaa atgacaagag aatgagaggc
     1621 ttgtcataat aatttttctt taaatggctg attttccgtc atcagatttt gcataaacac
     1681 cggcgatcgc actatttgct aaaatttcgt cccgccacca accactggca agccccgcgg
     1741 tctgttcatt ccagccaatc cagacgggtt caaagagggt ttgggcgacc actttcttct
     1801 cgattaacgc accactatcg agaaaacgtt gcgccagata acggggtaaa tagccgcagc
     1861 cgaggccgct aatttgcagc tccagcttgg ttttaaaatc gaaaacggta atagcttctt
     1921 gttcgtcgag aagttgtgag gccgtagaag cgccggcctg tgcagtgtcc cccaccacaa
     1981 tcgcccgata tcgtttaatg atacgacggt ttaacggttc ttcttccagg gctaacggat
     2041 gatgaggcgc gaccgcaaag acttgttcca gatcgcccaa ccgagagaaa ccaaactcac
     2101 tggaagatgg tggctcatgc atcgcgccaa caataatatc cgctcgcccc tgagtcaggg
     2161 catcccatga accaccgagt acaccattga taaacttcag gcgcgtcacg ctatgatgtt
     2221 gataaaaagc ctcaatgagt ggcgcaagca gagaaaaagg gaaggtgtcg tccacgccaa
     2281 tcaccagctc gttttcccag ccttcatgca gtttgatcgc ctgcttttcc agttctcgca
     2341 cggtatgcag aacttcccgc cctttctcta ataacatttt tccggtgcgg gtgaatttag
     2401 cgcggtgtcc gctgcgatca agcaattgaa tattaaggtc gctttccagc ttgtgaacgg
     2461 tgtaactcag agcggacggc gtcttgtaga gtttggccga cgccgccgca aaactgcctt
     2521 ctttttctaa ggcatcgagg atcatcaaca catccagcag tggtttcata cacgccccct
     2581 tgcagtgtat gcggcgtcct gctggcgtga tcgtttactc cagcggcatt accagcggat
     2641 cgggatactg gtattcaaat cccagttcat tacaaatccg actgccatca ataatcttac
     2701 ctttgccgct gtccagacta tttctgaact gcggaggttc cagccccaat aaacgggcca
     2761 tctgcggata gaaaacatta cgcgcagggt gagctggcgc acatatatta tagatgtgtc
     2821 cgcctttagg tgcctgtaac aacagagtga tagcgccaat aacatcttct aaatggacta
     2881 aattaacacc atgttcacca tcaggggcgg tttttccggc aaagaagcgt ccgggatgac
     2941 gtcccggtcc gaccaggccc gcaagacgca gaatatcgac cgaagtaccg ggtaaattgt
     3001 gcagccagtc ttcgagttct tctaatactc gtccactgtt ggtcaccgga ttacgcgggg
     3061 tggtttcttt caccgtgcct tgcgcgtcgc catagacaga tgtcgagctg gtaaaaataa
     3121 tgcggggaat acgatgggcc agcgcgctat ccactaactc ttgtaccgct tgtaaataga
     3181 actcatcgcc ggggccgcta cgacgtgccg gaagcgtaat gaccagcgca tcggcatcca
     3241 tcagggcatc cagatcgtca gaatcgcaaa ctaactcagg ctccatgcga agcagatagc
     3301 tatcaatgcc actcattcgg gccgcttcga caccatcttg tgtggtttta ctcccggtga
     3361 cttgccagcc tcgcgctgaa agtgacatcg ccagcggcat gcctaaccac cctaacccga
     3421 caattgcgac ctttttcatc cgttatctcc tgatacacct tagatctata aggctacgct
     3481 agcgtatcaa aactgacaat tcattctatg aatgaatctg ttcaataatg ataacgacat
     3541 gctgcaatga gcagtgaatc atcagtaacc gcgtatacca gacggtgctc ctctgtaatg
     3601 cgtcgggacc agaaacctga caaattatgt ttcaggggtt ctggtttccc cttaccttca
     3661 aatggtgttc tgcgggtatc tttgataagt tcattgatct ttttaacaat tcgcttatct
     3721 gtttcctgcc agtacagata atcatcccat gattcctcag accagattag tttcactcaa
     3781 taatgtcctt ttccgttcct ttgcctgatt tcaggctatc gattgagtcc atcaatctcc
     3841 gggcgttagc gggggaacgc agtagataag ccgtctcttc cagcgagttg tattcttcga
     3901 gtgacatcag aacacaagcc tctccattct gacgagtaat gaggatcggg gcatgatctt
     3961 caacggcttt catcattgtt gccgacaaat tctgacgcgc ttcgctgtag ctaattgtac
     4021 gcatgtaaat ctcctctttt gtacagttca ttgtacaatg atgggtgtta attcactatt
     4081 tattaattag tttgtagatc aagacattgc cagcgagacg aaaaatcaga ctttgctctt
     4141 tttggtgctg tcaagttaga ggacagtcct cttagccccc tccttttccc gctcattcat
     4201 taaacaaatc catcgccata aaatatataa aaaagccctt gctttctaac gtgaaagtgg
     4261 tttaggttaa aagacatcag ttgaataaac attcacagag acttttatga cacgcgttca
     4321 atttaaacac catcatcacc atcatcctga ctagtctttc aggcgatgtg tgctggaaga
     4381 cattcagatc ttccagtggt gcatgaacgc atgagaaagc ccccggaaga tcaccttccg
     4441 ggggcttttt tattgcgcgg ttgttaacgg ttcagacagg tttaaagagg aataagaaaa
     4501 tgacagacaa cactcgttta cgcatagcta tgcagaaatc cggccgttta agtgatgact
     4561 cacgcgaatt actggcgcgc tgtggcatta aaatcaatct tcacacccag cgcctgatcg
     4621 cgatggcaga aaacatgccg attgatattc tgcgcgtgcg tgacgatgac attcccggtc
     4681 tggtaatgga cggcgtggta gaccttggga ttatcggcga aaacgtgctg gaagaagagc
     4741 tgcttaaccg ccgcgcccag ggtgaagatc cgcgctactt taccttgcgt cgtctggatt
     4801 tcggcggctg ccgcctttcg ctggcaacgc cggttgatga agcctgggac ggtccgctct
     4861 ccttaaatgg taaacgtatc gccacctctt atcctcacct gctcaagcgt tatctcgacc
     4921 agaaaggtat ctcttttaaa tcctgcttac tgaacggctc tgtcgaagtt gcgccgcgtg
     4981 ccggactggc ggatgcgatt tgcgatctgg tttccaccgg tgccacgctg gaagctaacg
     5041 gcctgcgcga agtcgaagtt atctaccgct cgaaagcctg cctgatccag cgcgatggcg
     5101 aaatggaaga atccaaacag caactgatcg acaaactgct gacccgtatt cagggtgtga
     5161 tccaggcgcg cgaatcaaaa tacatcatga tgcacgcacc gaccgaacgt ctggatgaag
     5221 tcatcgccct gctgccaggt gccgaacgcc caaccattct gccgctggcg ggtgaccaac
     5281 aacgcgtagc gatgcacatg gtcagtagcg aaaccctgtt ctgggaaacc atggaaaaac
     5341 tgaaagcgct gggtgccagt tcaatcctgg tcctgccgat tgagaagatg atggagtgat
     5401 cgccatgagc tttaacacaa tcattgactg gaatagctgt actgcggagc aacaacgcca
     5461 gctgttaatg cgcccggcga tctccgcttc tgaaagcatt acccgcaccg ttaacgatat
     5521 tctcgataac gtgaaagcac gcggcgatga cgccctgcgg gaatacagcg cgaagtttga
     5581 taaaaccacg gttaccgcgc tgaaggtgtc tgctgaggag atcgccgccg ccagcgaacg
     5641 ccttagcgac gagctaaaac aggcgatggc ggtggcagta aaaaatattg aaaccttcca
     5701 cactgcgcaa aaactgccgc cggtagatgt tgaaacacag ccaggcgtgc gttgccagca
     5761 agtcacgcgc ccggtagctt cagttgggtt gtatattcct ggcggctccg ccccactctt
     5821 ctcaacggta ttaatgctgg caactcctgc gcgtattgcg ggctgtaaaa aagtggtgtt
     5881 gtgctcaccg ccgccgattg ccgatgagat cctttatgcg gcgcagctgt gcggtgtgca
     5941 ggacgtgttt aacgtcggcg gcgcacaggc cattgccgct ctggcgtttg gtacggaatc
     6001 tgtgccgaaa gtggacaaaa tcttcgggcc gggtaacgcc tttgtcaccg aagcgaaacg
     6061 tcaggtgagc cagcgtcagg acggtgcggc gatcgatatg cccgcaggcc cgtcggaagt
     6121 gctggtgatt gctgacagcg gcgctacgcc agatttcgtg gcttctgatt tgctttctca
     6181 ggctgaacac ggcccggact cacaggtgat tttactgacg cccgctgctg atatggcgcg
     6241 tcgcgttgcc gaggccgtcg aacgccagtt ggcagaactg ccgcgtgccg aaaccgcccg
     6301 ccaggcactg aacgccagcc gcctaatcgt gaccaacgat ttagcacagt gcgtggagat
     6361 ctccaaccag tacggcccgg agcacctgat cattcagacc cgcaacgctc gcgatctggt
     6421 cgatagcatc accagcgccg gttcggtatt tcttggtgac tggtcaccgg aatcggcagg
     6481 tgattacgcc tccggcacca accacgttct gccgacttac ggttacaccg ccacctgttc
     6541 cagcctcggg ctggcagatt tccagaagcg catgaccgtg caggaactgt cgaaagaagg
     6601 cttttctgcg ctggcttcaa ccatagaaac actggccgcc gccgagcgcc tgaccgccca
     6661 taaaaatgcc gttactttgc gtgttaacgc ccttaaggag caagcatgag caccgtgact
     6721 attaccgatt tagcgcgtga aaacgtccgc aacctgacgc cgtatcagtc ggcgcgtcgt
     6781 ctgggcggta acggcgacgt ctggctgaac gccaacgaat atcccgcagc cgtggagttt
     6841 cagcttactc aacaaacgct caaccgctac ccggaatgcc agccgaaagc ggtgattgaa
     6901 aattacgcgc aatatgcagg cgtaaaaccg gagcaggtgc tggtcagccg tggcgcggac
     6961 gaaggtattg aactgctgat tcgcgctttt tgcgaaccgg gtaaagacgc catcctctac
     7021 tgcccgccaa cgtacggcat gtacagcgtc agcgccgaaa cgattggcgt cgagtgtcgc
     7081 acagtgccga cgcaggacaa ctggcaactg gacttacagg gcatttccga caagctggac
     7141 ggcgtaaaag tggtctatgt ttgcagcccc aacaacccga ccgggcaact gatcaatccg
     7201 caggattttc gcactctgct ggagctaacc cgcggaaaag ccattgtggt tgccgatgaa
     7261 gcctatatag agttttgtcc acaggcatcg ctggctggct ggctggcgga atatccgcac
     7321 ctggctattt tgcgcacact atcgaaagct tttgctctgg cggggcttcg ttgcggattt
     7381 acgctggcaa acgaagaagt catcaacctg ctgatgaaag tgatcgcccc ctacccgctc
     7441 tcgacgccgg ttgccgacat tgcggcccag gcgttaagcc cgcaggggat cgtcgccatg
     7501 cgcgaacggg tggcgcaaat tattgcagaa cgcgaatacc tgattgccgc actgaaagag
     7561 atcccctgcg tggagcaggt ttttgactct gaaaccaact acattctggc gcgctttaaa
     7621 gcctccagtg cagtgtttaa atctttgtgg gatcagggca ttatcttacg tgatcagaat
     7681 aaacaaccct ctttaagcgg ctgcctgcga attaccgtcg gaacccgtga agaaagccag
     7741 cgcgtcattg acgccttacg tgcggagcaa gtttaatgag tcagaagtat ctttttatcg
     7801 atcgcgatgg aaccctgatt agcgaaccgc cgagtgattt tcaggtggac cgttttgaca
     7861 aactcgcctt tgaaccgggc gtgatcccgg agctgctgaa gttgcaaaaa gcgggctaca
     7921 aactggtgat gatcactaat caggatggtc tgggaacaca aagtttcccg caggcggatt
     7981 tcgatggccc gcacaacctg atgatgcaga tcttcacctc gcaaggcgta cagtttgatg
     8041 aagtgctgat ttgtccgcac ctgcccgccg atgagtgcga ctgccgtaag ccgaaagtaa
     8101 aactggtgga gcgttatctc gctgagcaag cgatggatcg tgccaatagt tatgtgattg
     8161 gcgatcgcgc gaccgacatt caactggcgg aaaacatggg tattaatggt ttacgctacg
     8221 accgcgaaac cctgaactgg ccaatgattg gcgagcaact cactaaacga gaccgttacg
     8281 cccatgtagt gcgtaacacc aaagagacgc aaattgacgt ccaggtgtgg ctggatcgcg
     8341 aaggtgacag caagattaac accggcgttg gcttctttga tcacatgctg gatcagatcg
     8401 ccacccacgg cggtttccgt atggaaatca acgtcaaagg cgacctctat atcgacgatc
     8461 accacacagt cgaagatacc ggcctggcgc tgggtgaagc gttaaaaatt gcccttggcg
     8521 acaaacgcgg tatttgccgc tttggttttg tgctaccgat ggacgaatgc cttgcccgct
     8581 gcgcgctgga tatctctggt cgcccgcacc tggaatataa agccgagttt acctaccagc
     8641 gcgtgggcga tctcagcacc gaaatgatcg agcacttctt ccgttcgctc tcttacacca
     8701 tgggcgtgac gctacacctg aaaaccaaag gtaaaaacga tcatcaccgt gtagagagcc
     8761 tgttcaaagc ctttggtcgc accctgcgcc aggccatccg cgtggaaggc gacaccctgc
     8821 cctcgtcaaa aggagtgctg taatgaacgt ggtgatcctt gataccggct gcgccaatct
     8881 gaactcggtg aagtctgcca ttgcgcgcca cggttatgaa cccaaagtca gccgtgaccc
     8941 ggacgtcgtg ttgctggccg ataaactgtt tttacccggc gtaggcaccg cacaagcagc
     9001 gatggatcag gtgcgtgagc gcgaactgtt tgatctcatc aaagcctgta cccaaccggt
     9061 gctgggaatc tgcttaggga tgcaactgct ggggcggcgc agcgaagaga gcaacggcgt
     9121 cgacttgctg ggcatcattg acgaagacgt gccgaaaatg accgactttg ctctgccact
     9181 gccacatatg ggctggaacc gcgtttatcc gcaggcaggc aaccgcctgt ttcagggaat
     9241 tgaagacggc gcgtactttt actttgttca cagttacgca atgccggtca atccgtggac
     9301 catcgcccag tgtaattacg gcgaaccgtt caccgcggcg gtacaaaaag ataacttcta
     9361 cggcgtgcag ttccacccgg agcgttctgg tgccgctggc gctaagttgc tgaaaaactt
     9421 cctggagatg tgatgattat tccggcatta gatttaatcg acggcactgt ggtgcgtctc
     9481 catcagggcg attacggcaa acagcgcgat tatggtaacg acccgctgcc gcgattgcag
     9541 gattacgccg cgcagggtgc agaattgttg catctggtgg atctgaccgg ggcaaaagat
     9601 ccggctaaac gtcaaatccc gctgattaaa accctggtgg cgggcgttaa cgtgccggtg
     9661 caggttggcg gtggcgtgcg tagcgaagaa gacgtggcgg cgttactgga agcaggcgtt
     9721 gcgcgcgtgg tggttggctc caccgcggtg aaatcaccag agatggtgaa aggctggttt
     9781 gaacgtttcg gtgccgatgc cttagtgctg gcgctggatg tccgtattga cgagcaaggc
     9841 aacaagcagg tggcagtcag cggctggcaa gagaactcgg gcgtttcact ggaacaactg
     9901 gtggaaagct atctgcccgt cggcctgaaa catgtgctgt gtaccgatat ctcgcgcgac
     9961 ggcacgctgg caggttctaa cgtctcttta tatgaagaag tgtgcgccag atatccgcag
    10021 gtggcgtttc agtcctccgg cggtattggc gacattgatg atgtggcggc cctgcgtggt
    10081 actggcgtgc gcggcgtaat agttggtcgt gcattactgg aaggtaaatt caccgtgaag
    10141 gaggccatcg catgctggca aaacgcataa taccgtgtct cgacgtccgt gatggtcagg
    10201 tggtgaaagg cgtacagttt cgcaaccatg aaatcattgg cgatatcgtg ccgctggcaa
    10261 aacgctacgc tgaagaaggt gcagacgaac tggtgttcta cgatatcacc gcttccagcg
    10321 atggccgtgt ggtagataaa agctgggtat ctcgcgtggc ggaagtgatc gacattccgt
    10381 tctgcgtggc gggtgggatt aagtctctgg aagatgccgc gaaaattctt tcctttggcg
    10441 cggataaaat ttccatcaac tcccctgcgc tggcagaccc aacattaatt actcgcctgg
    10501 cggatcgttt tggcgtgcag tgtattgtgg tgggtattga tacctggtac gacgctgaaa
    10561 ccggtaaata tcatgtgaat caatataccg gcgatgaaag ccgcacccgc gtcactcagt
    10621 gggaaacact cgactgggta caggaagtgc aaaaacgcgg tgccggagaa atcgtcctca
    10681 acatgatgaa tcaggacggc gtgcgtaacg gttacgacct cgagcagctg aaaaaagtgc
    10741 gagaagtttg ccgcgtcccg ctgattgcct ccggtggcgc gggcaccatg gaacacttcc
    10801 tcgaagcctt ccgtgatgcc gacgttgacg gcgcgctggc ggcttccgta ttccacaaac
    10861 aaataatcaa tattggtgaa ttaaaagcgt acctggcaac acagggcgtg gagatcagga
    10921 tatgttaaca gaacaacaac gtcgcgaact ggactgggaa aaaaccgacg gactgatgcc
    10981 ggtgattgtg caacacgcgg tatctggcga agtgctaatg ctgggctata tgaacccgga
    11041 agccttagac aaaaccatcg aaagcggcaa agtcactttc ttctcgcgca ctaaacagcg
    11101 actgtggacc aaaggcgaaa cgtcgggcaa tttcctcaac gtagtaaata ttgcgccgga
    11161 ctgcgacaac gacacgctac tggtactggc gaatcccatc gggccaacct gccacaaagg
    11221 caccagcagc tgcttcggcg acaccgctca ccagtggctg ttcctgtatc aactggaaca
    11281 attgctcgcc gaacgcaaat ctgccgatcc ggaaacctct tacacagcca aactgtatgc
    11341 cagcggcacc aaacgcattg cacagaaagt gggcgaagag ggcgtggaaa ccgcactagc
    11401 agcaacggta catgaccgct ttgaactcac caacgaggcg tctgacttga tgtatcacct
    11461 gttggtgttg ttgcaggatc aggatctgga tttaacaacg gtgattgaga acctgcgtaa
    11521 acggcatcag tgagttgcgg ggtaagcgga tgcgatgttg ttgtcgcatc cggcaaaaaa
    11581 aacgggcaag gtgtcaccac cctgcccgtt ttctttaaaa ccaaaaagat tacttcgcgt
    11641 tgtaattacg cagagcgtta cgccccagca caatccccgc gccaaccatg ccgcccagca
    11701 gcaccgccag aatcaaagtg attgcctttt tcgggctatc gcgacgaata ggtaacgtcg
    11761 gtttcatcac atagcggtaa gcatgaatat caagatcatc aacttttaag ctttcgatat
    11821 ccagcaggtt ttgacgtgtc tgatagtagt ttggtgagaa caccaacgga cgagtcgctt
    11881 catgcttaat catcgactcc agcgcttcgc tccccaaaag gaacaacgta tcttgtgtca
    11941 catcttcagt ctgctgaacc tgtggctttg tcacctgcgc ctgattcgca tactgcaacg
    12001 cttcctgaat ctgacggata cgcagatctt tctgctcctg cgcaactact tcctgagttt
    12061 tcagagaatc ctgcaagttt ttcatacgca gcgcgatgtt gtctttcagg tcttttccca
    12121 gttcttcatt tacctggtcg tcaacttgct ggatatactt cgccagctgc ttctgagcac
    12181 cttcaggtgt ttcaccaaca taagacaccg ctaaaggtaa tgactgattt ttgacggtag
    12241 gctcaatggt cagcttttct gcttcttcct ggttatccag cgtttccgct aatgctgaga
    12301 atgcagtgct gtaacgtccg ataagtgaag cctgaatgtc ggagactttc ggtacagccg
    12361 atccgtagat cacattcatc gcgttggtat aaccagcgat ttgccccaca tcaggttgag
    12421 taacgatagc tgtggaagtc cacttttctt tggcaacggc cagataccct atagccaggg
    12481 caatagcaac aatgacagag atgataattg tcatcttgcc acgccacaac tgcaccagta
    12541 aatcaatcaa atcaatctgt tccgggtcat ggttttgccc agaaacatta ttattttcta
    12601 ctctcatcat taccctaacg gacgaaataa ttttaagtcg ccatagttta gcg
//
LOCUS       DAZKHF020000128        12547 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2201S31241
            SAMN43267800-rid25106413.denovo.129, whole genome shotgun sequence.
ACCESSION   DAZKHF020000128 DAZKHF020000000
VERSION     DAZKHF020000128.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267800
            Sequence Read Archive: SRR30307263
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 12547)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12547)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 12547)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350581.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350581.2
            Genome Coverage       :: 151x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:21:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,652
            CDSs (total)                      :: 5,557
            Genes (coding)                    :: 5,320
            CDSs (with protein)               :: 5,320
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 237
            CDSs (without protein)            :: 237
            Pseudo Genes (ambiguous residues) :: 0 of 237
            Pseudo Genes (frameshifted)       :: 102 of 237
            Pseudo Genes (incomplete)         :: 130 of 237
            Pseudo Genes (internal stop)      :: 52 of 237
            Pseudo Genes (multiple problems)  :: 41 of 237
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12547
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267800-rid25106413.denovo.129"
                     /serotype="O 26:H11"
                     /isolate="2201S31241"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-01"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            117..986
                     /gene="robA"
                     /locus_tag="ACHOSC_RS14555"
                     /old_locus_tag="pgaptmp_002911"
     CDS             117..986
                     /gene="robA"
                     /locus_tag="ACHOSC_RS14555"
                     /old_locus_tag="pgaptmp_002911"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MDR efflux pump AcrAB transcriptional activator
                     RobA"
                     /protein_id="HGU4548815.1"
                     /translation="MDQAGIIRDLLIWLEGHLDQPLSLDNVAAKAGYSKWHLQRMFKD
                     VTGHAIGAYIRARRLSKSAVALRLTARPILDIALQYRFDSQQTFTRAFKKQFAQTPAL
                     YRRSPEWSAFGIRPPLRLGEFTMPEHKFVTLEDTPLIGVTQSYSCSLEQISDFRHEMR
                     YQFWHDFLGNAPTIPPVLYGLNETRPSQDKDDEQEVFYTTALAQDQADGYVLTGHPVM
                     LQGGEYVMFTYEGLGTGVQEFILTVYGTCMPMLNLTRRKGQDIERYYPAEDAKAGDRP
                     INLRCELLIPIRR"
     gene            complement(983..1630)
                     /gene="gpmB"
                     /locus_tag="ACHOSC_RS14560"
                     /old_locus_tag="pgaptmp_002912"
     CDS             complement(983..1630)
                     /gene="gpmB"
                     /locus_tag="ACHOSC_RS14560"
                     /old_locus_tag="pgaptmp_002912"
                     /EC_number="5.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004619 - phosphoglycerate mutase activity
                     [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-diphosphoglycerate-dependent
                     phosphoglycerate mutase GpmB"
                     /protein_id="HGU4548816.1"
                     /translation="MLQVYLVRHGETQWNAERRIQGQSDSPLTAKGEQQAMQVATRAK
                     ELGITHIISSDLGRTRRTAEIIAQACGCDIIFDSRLRELNMGVLEKRHIDSLTEEEEN
                     WRRQLVNGTVDGRIPEGESMQELSDRVNAALESCRDLPQGSRPLLVSHGIALGCLVST
                     ILGLPAWAERRLRLRNCSISRVDYQESLWLASGWVVETAGDISHLDAPALDELQR"
     gene            1682..2203
                     /gene="yjjX"
                     /locus_tag="ACHOSC_RS14565"
                     /old_locus_tag="pgaptmp_002913"
     CDS             1682..2203
                     /gene="yjjX"
                     /locus_tag="ACHOSC_RS14565"
                     /old_locus_tag="pgaptmp_002913"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001649724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inosine/xanthosine triphosphatase"
                     /protein_id="HGU4548817.1"
                     /translation="MHQVVCATTNPAKIQAILQAFHEIFGEGSCHIASVAVESGVPEQ
                     PFGSEETRAGARNRVANARRLLPEADFWVAIEAGIDGDSTFSWVVIENASQRGEARSA
                     TLPLPAVILEKVREGEALGPVMSRYTGIDEIGRKEGAIGVFTAGKLTRASVYHQAVIL
                     ALSPFHNAVYQAL"
     gene            complement(2288..2614)
                     /gene="trpR"
                     /locus_tag="ACHOSC_RS14570"
                     /old_locus_tag="pgaptmp_002914"
     CDS             complement(2288..2614)
                     /gene="trpR"
                     /locus_tag="ACHOSC_RS14570"
                     /old_locus_tag="pgaptmp_002914"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trp operon repressor"
                     /protein_id="HGU4548818.1"
                     /translation="MAQQSPYSAAMAEQRHQEWLRFVDLLKNAYQNDLHLPLLNLMLT
                     PDEREALGTRVRIVEELLRGEMSQRELKNELGAGIATITRGSNSLKAAPVELRQWLEE
                     VLLKSD"
     gene            complement(2704..4641)
                     /gene="sltY"
                     /locus_tag="ACHOSC_RS14575"
                     /old_locus_tag="pgaptmp_002915"
     CDS             complement(2704..4641)
                     /gene="sltY"
                     /locus_tag="ACHOSC_RS14575"
                     /old_locus_tag="pgaptmp_002915"
                     /EC_number="4.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418809.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA];
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_function: GO:0008933 - lytic transglycosylase activity
                     [Evidence IEA];
                     GO_process: GO:0000270 - peptidoglycan metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="murein transglycosylase"
                     /protein_id="HGU4548819.1"
                     /translation="MEKAKQVTWRLLAAGVCLLTVSSVARADSLDEQRSRYAQIKQAW
                     DNRQMDVVEQMMPGLKDYPLYPYLEYRQITDDLMNQPAVTVTNFVRANPTLPPARTLQ
                     SRFVNELARREDWRGLLAFSPEKPGTTEAQCNYYYAKWNTGQSEEAWQGAKELWLTGK
                     SQPNACDKLFSVWRASGKQDPLAYLERIRLAMKAGNTGLVTVLAGQMPADYQTIASAI
                     ISLANNPNTVLTFARTTGATDFTRQMAAVAFASVARQDAENARLMIPSLAQAQQLNED
                     HIQELRDIVAWRLMGNDVTDEQAKWRDDAIMRSQSTSLIERRVRMALGTGDRRGLNTW
                     LARLPMEAKEKDEWRYWQADLLLERGREAEAKEILHQLMQQRGFYPMVAAQRIGEEYE
                     LKIDKAPQNVDSALTQGPEMARVRELMYWNLDNTARSEWANLVKSKSKTEQAQLARYA
                     FNNQWWDLSVQATIAGKLWDHLEERFPLAYNDLFKRYTSGKEIPQSYAMAIARQESAW
                     NPKVKSPVGASGLMQIMPGTATHTVKMFSIPGYSSPGQLLDPETNINIGTSYLQYVYQ
                     QFGNNRIFSSAAYNAGPGRVRTWLGNSAGRIDAVAFVESIPFSETRGYVKNVLAYDAY
                     YRYFMGDKPTLMSTTEWGRRY"
     gene            4852..6519
                     /gene="ettA"
                     /locus_tag="ACHOSC_RS14580"
                     /old_locus_tag="pgaptmp_002916"
     CDS             4852..6519
                     /gene="ettA"
                     /locus_tag="ACHOSC_RS14580"
                     /old_locus_tag="pgaptmp_002916"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017145039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0045900 - negative regulation of
                     translational elongation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy-dependent translational throttle protein
                     EttA"
                     /protein_id="HGU4548820.1"
                     /translation="MAQFVYTMHRVGKVVPPKRHILKNISLSFFPGAKIGVLGLNGAG
                     KSTLLRIMAGIDKDIEGEARPQPDIKIGYLPQEPQLNPEHTVRESIEEAVSEVVNALK
                     RLDEVYALYADPDADFDKLAAEQGRLEEIIQAHDGHNLNVQLERAADALRLPDWDAKI
                     ANLSGGERRRVALCRLLLEKPDMLLLDEPTNHLDAESVAWLERFLHDFEGTVVAITHD
                     RYFLDNVAGWILELDRGEGIPWEGNYSSWLEQKDQRLAQEASQEAARRKSIEKELEWV
                     RQGTKGRQSKGKARLARFEELNSTEYQKRNETNELFIPPGPRLGDKVLEVSNLRKSYG
                     DRLLIDDLSFSIPKGAIVGIIGPNGAGKSTLFRMISGQEQPDSGTITLGETVKLASVD
                     QFRDSMDNSKTVWEEVSGGLDIMKIGNTEMPSRAYVGRFNFKGVDQGKRVGELSGGER
                     GRLHLAKLLQVGGNMLLLDEPTNDLDIETLRALENALLEFPGCAMVISHDRWFLDRIA
                     THILDYQDEGKVEFFEGNFTEYEEYKKRTLGADALEPKRIKYKRIAK"
     gene            complement(6826..8058)
                     /gene="nadR"
                     /locus_tag="ACHOSC_RS14585"
                     /old_locus_tag="pgaptmp_002917"
     CDS             complement(6826..8058)
                     /gene="nadR"
                     /locus_tag="ACHOSC_RS14585"
                     /old_locus_tag="pgaptmp_002917"
                     /EC_number="2.7.1.22"
                     /EC_number="2.7.7.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418807.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000309 - nicotinamide-nucleotide
                     adenylyltransferase activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0050262 - ribosylnicotinamide kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multifunctional transcriptional
                     regulator/nicotinamide-nucleotide
                     adenylyltransferase/ribosylnicotinamide kinase NadR"
                     /protein_id="HGU4548821.1"
                     /translation="MSSFDYLKTAIKQQGCTLQQVADASGMTKGYLSQLLNAKIKSPS
                     AQKLEALHRFLGLEFPRQKKTIGVVFGKFYPLHTGHIYLIQRACSQVDELHIIMGFDD
                     TRDRALFEDSAMSQQPTVPDRLRWLLQTFKYQKNIRIHAFNEEGMEPYPHGWDVWSNG
                     IKKFMAEKGIQPDLIYTSEEADAPQYMEHLGIETVLVDPKRTFMSISGAQIRENPFRY
                     WEYIPTEVKPFFVRTVAILGGESSGKSTLVNKLANIFNTTSAWEYGRDYVFSHLGGDE
                     IALQYSDYDKIALGHAQYIDFAVKYANKVAFIDTDFVTTQAFCKKYEGREHPFVQALI
                     DEYRFDLVILLENNTPWVADGLRSLGSSVDRKEFQNLLVEMLEENNIEFVRVEEDDYD
                     SRFLRCVELVREMMGEQR"
     gene            complement(8079..9461)
                     /gene="radA"
                     /locus_tag="ACHOSC_RS14590"
                     /old_locus_tag="pgaptmp_002918"
     CDS             complement(8079..9461)
                     /gene="radA"
                     /locus_tag="ACHOSC_RS14590"
                     /old_locus_tag="pgaptmp_002918"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001399683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003684 - damaged DNA binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadA"
                     /protein_id="HGU4548822.1"
                     /translation="MAKAPKRAFVCNECGADYPRWQGQCSACHAWNTITEVRLAASPT
                     VARNERLSGYAGSAGVAKVQKLSDISLEELPRFSTGFKEFDRVLGGGVVPGSAILIGG
                     NPGAGKSTLLLQTLCKLAQQMKTLYVTGEESLQQVAMRAHRLGLPTDNLNMLSETSIE
                     QICLIAEEEQPKLMVIDSIQVMHMADVQSSPGSVAQVRETAAYLTRFAKTRGVAIVMV
                     GHVTKDGSLAGPKVLEHCIDCSVLLDGDADSRFRTLRSHKNRFGAVNELGVFAMTEQG
                     LREVSNPSAIFLSRGDEVTSGSSVMVVWEGTRPLLVEIQALVDHSMMANPRRVAVGLE
                     QNRLAILLAVLHRHGGLQMADQDVFVNVVGGVKVTETSADLALLLAMVSSLRDRPLPQ
                     DLVVFGEVGLAGEIRPVPSGQERISEAAKHGFRRAIVPAANVPKKAPEGMQIFGVKKL
                     SDALSVFDDL"
     gene            complement(9510..10478)
                     /gene="serB"
                     /locus_tag="ACHOSC_RS14595"
                     /old_locus_tag="pgaptmp_002919"
     CDS             complement(9510..10478)
                     /gene="serB"
                     /locus_tag="ACHOSC_RS14595"
                     /old_locus_tag="pgaptmp_002919"
                     /EC_number="3.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0036424 - L-phosphoserine phosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0006564 - L-serine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoserine phosphatase"
                     /protein_id="HGU4548823.1"
                     /translation="MPNITWCDLPEDVSLWPGLPLSLSGDEVMPLDYHAGRSGWLLYG
                     RGLDKQRLTQYQSKLGAAMVIVAAWCVEDYQVIRLAGSLTARATRLAHEAQLDVAPLG
                     KIPHLRTPGLLVMDMDSTAIQIECIDEIAKLAGTGEMVAEVTERAMRGELDFTASLRS
                     RVATLKGADANILQQVRENLPLMPGLTQLVLKLETLGWKVAIASGGFTFFAEYLRDKL
                     RLTAVVANELEIMDGKFTGNVIGDIVDAQYKAKTLTRLAQEYEIPLAQTVAIGDGAND
                     LPMIKAAGLGIAYHAKPKVNEKTEVTIRHADLMGVFCILSGSLNQK"
     gene            10584..11228
                     /locus_tag="ACHOSC_RS14600"
                     /old_locus_tag="pgaptmp_002920"
     CDS             10584..11228
                     /locus_tag="ACHOSC_RS14600"
                     /old_locus_tag="pgaptmp_002920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtjB family periplasmic protein"
                     /protein_id="HGU4548824.1"
                     /translation="MARTKLKFRLHRAVIVLFCLALLVALMQGASWFSQNHQRQRNPQ
                     LEELARTLARQVTLNVAPLMRTDSPDEKRIQAILDQLTDESRILDAGVYDEQGDLIAR
                     SGESVEVRDRLALDGKKAGGYFNQQIVEPIAGKNGPLGYLRLTLDTHTLATEAQQVDN
                     TTNILRLMLLLSLAIGVVLTRTLLQGKRTRWQQSPFLLTASKPVPEEEESEKKE"
     gene            11256..12272
                     /gene="lplA"
                     /locus_tag="ACHOSC_RS14605"
                     /old_locus_tag="pgaptmp_002921"
     CDS             11256..12272
                     /gene="lplA"
                     /locus_tag="ACHOSC_RS14605"
                     /old_locus_tag="pgaptmp_002921"
                     /EC_number="6.3.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoate--protein ligase LplA"
                     /protein_id="HGU4548825.1"
                     /translation="MSTLRLLISDSYDPWFNLAVEECIFRQMPATQRVLFLWRNADTV
                     VIGRAQNPWKECNTRRMEEDNVRLARRSSGGGAVFHDLGNTCFTFMAGKPEYDKTIST
                     SIVLNALNALGVSAEASGRNDLVVKTVEGDRKVSGSAYRETKDRGFHHGTLLLNADLS
                     RLANYLNPDKKKLAAKGITSVRSRVTNLTELLPGITHEQVCEVITKAFFAHYGERVEA
                     EIISPDKTPDLPNFAETFARQSSWEWNFGQAPAFSHLLDERFSWGGVELHFDVEKGHI
                     TRAQVFTDSLNPAPLEALAGRLQGCLYRADMLQQECEALLVDFPDQEKELRELSTWIA
                     GAVR"
     gene            12304..>12547
                     /locus_tag="ACHOSC_RS14610"
                     /old_locus_tag="pgaptmp_002922"
     CDS             12304..>12547
                     /locus_tag="ACHOSC_RS14610"
                     /old_locus_tag="pgaptmp_002922"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HGU4548826.1"
                     /translation="MIDLENQEREIINLMLSQRISWLAAVRIRHKLSLAEVSKMLGIS
                     INSLKQIEKTERLSSNIKSKMAEIYGCPPELLICPSW"
BASE COUNT         2891 a         3534 c         3263 g         2859 t
ORIGIN      
        1 caagccctaa aacatactct actaagaaaa aaacactgaa tgctaaaaca gcaaaaaatg
       61 ctattatcca attacctgat gtcaggtgct cgttgttgaa aggatgagga tattttatgg
      121 atcaggccgg cattattcgc gaccttttaa tctggctgga aggtcatctg gatcagcccc
      181 tgtcgctcga caatgtagcg gcgaaagcag gttattccaa gtggcactta cagagaatgt
      241 ttaaagatgt cactggccat gctattggcg cgtatattcg tgctcgtcgt ttgtcgaaat
      301 cggcggtcgc actacgcctg actgcgcgtc cgattctgga catcgcgctg caataccgct
      361 tcgactctca acagacattt acccgcgcat tcaagaagca gtttgcccag actcctgcac
      421 tttaccgccg ttctcctgaa tggagcgcct ttggtattcg cccgccgcta cgtctgggtg
      481 aattcactat gccagagcac aaatttgtca ccctggaaga tacgccgctg attggtgtta
      541 cccagagcta ctcctgttcg ctggagcaaa tctctgattt ccgccatgaa atgcgttatc
      601 agttctggca cgattttctc ggcaacgcgc cgaccattcc gccggtgctc tacggcctga
      661 atgaaacgcg tccgagtcag gataaagacg acgaacaaga agtattctat accaccgcgt
      721 tagcccagga tcaggcagat ggctatgtac tgacggggca tccggtgatg ctgcagggcg
      781 gcgaatatgt gatgtttacc tatgaaggtc tgggaaccgg cgtgcaggag tttatcctga
      841 cggtatatgg aacatgcatg ccaatgctta acctgacgcg ccgtaaaggt caggatattg
      901 agcgatacta cccggcagaa gatgccaaag caggagatcg cccaattaat ctgcgctgtg
      961 aactgctgat tccgatccgt cgttaacgct gcaactcatc taatgcaggg gcgtccagat
     1021 gcgagatgtc ccctgcggtt tcaacaaccc agccagacgc cagccacagg ctttcctgat
     1081 aatccacgcg cgaaatagaa cagttacgca gacgtaagcg gcgctctgcc catgctggta
     1141 atccgagaat cgtactcacc aggcatccca gtgcaatacc atgactcacc agcagcggtc
     1201 ggcttccctg cggtaagtcc cggcaagatt ccagtgccgc attgacgcga tcgctgagct
     1261 cctgcattga ctcgccttca ggaatacgcc cgtcaacggt gccattgacc agctgccgac
     1321 gccagttctc ttcttcttcg gtcagagaat cgatatgtct tttttccagc acacccatgt
     1381 ttaattcacg caggcgagaa tcaaagatga tgtcgcagcc gcaggcctgg gcgatgattt
     1441 ccgccgtacg ccgggtgcgt cctaaatcgc tactgatgat atgcgtaatg ccaagctctt
     1501 tggcacgggt tgccacctgc atcgcctgtt gctcaccttt ggcggtcagc gggctgtcag
     1561 actggccctg aatacgtcgc tcggcgttcc actgcgtttc accgtggcgg actaggtata
     1621 cctgtaacat gctttttttc cgtatactgc tgtaacgtta attttctgag taatgctgat
     1681 tatgcaccaa gttgtctgtg cgaccaccaa tcccgctaaa attcaggcca ttctgcaggc
     1741 atttcacgag atcttcggcg aaggatcctg ccatattgca tccgttgccg tcgagagcgg
     1801 tgtaccggaa caaccctttg gcagtgagga aacgcgcgct ggcgcacgaa atcgggtagc
     1861 aaatgcccgc cgtttacttc cagaggctga tttttgggtg gcgattgaag ctggcatcga
     1921 tggtgacagc actttcagtt gggtggtgat tgaaaacgcc agccagcgcg gcgaggcgcg
     1981 ttcggctact ttgccgttgc ctgcggtgat tctggaaaaa gtgcgtgaag gcgaggcgct
     2041 cggccccgtg atgtcgcgtt ataccggtat tgatgagatt ggtcgtaagg aaggggcgat
     2101 cggcgtgttt accgccggga aactcacccg cgccagcgtc tatcaccagg cggtgatcct
     2161 ggcgctgagt ccgtttcata atgccgtgta tcaggcattg taggacggat aaggcgttca
     2221 cgccgcatcc ggcattcggt gcacgatgcc tgatgcgacg ctgccgcgtc ttatcaggcc
     2281 tacaaaatca atcgcttttc agcaacacct cttccagcca ctggcgcagc tcaacgggcg
     2341 cggctttcag gctgttagat ccacgcgtaa tcgtcgcgat gcccgcgccg agttcatttt
     2401 ttaactcacg ctggctcatt tcgccgcgca acagctcttc gacaatacgc acgcgagtcc
     2461 ccaacgcttc gcgctcatct ggcgtcagca tcaggtttaa caacggtaaa tggagatcgt
     2521 tttggtaggc attcttaagc aggtcgacaa aacgtaacca ctcctggtga cgctgttctg
     2581 ccatcgctgc tgaatagggt gattgttggg ccataatatg tcgccattgt tagcggggga
     2641 agcaaaatgc ctcccccggt tgtactcgct aaagagtacg atagcatatc ataaacgtgc
     2701 ggatcagtaa cgacgtcccc attccgtggt gctcatcaac gtcggtttat cccccatgaa
     2761 atagcggtag taagcgtcat aagccagcac gttcttcaca taaccgcgcg tctcggagaa
     2821 cggaatactc tcgacaaatg ccacagcgtc gatacgcccg gcgctgttgc caagccaggt
     2881 tcgcacccgt ccaggtccgg cgttataagc tgctgaggag aaaatacggt tattgccaaa
     2941 ctgctgataa acatattgca ggtaactggt gccaatgttg atattcgttt ccggatccag
     3001 caattgccca gggctgctat aaccaggaat agagaacatc ttcaccgtat gggtcgctgt
     3061 accaggcata atctgcatca ggccgctggc ccctaccggt gatttcactt tcggattcca
     3121 ggcgctctcc tgacgggcaa tcgccatcgc atagctttgc gggatctctt tcccactggt
     3181 gtaacgtttg aaaagatcgt tgtaagccag cgggaatcgc tcttccagat gatcccaaag
     3241 cttcccggcg atcgttgcct gaacgctaag atcccaccat tggttattga aagcataccg
     3301 agccagttga gcctgctctg tttttgactt gctcttcacc agattggccc actcgctacg
     3361 cgcggtgtta tcgagattcc agtacatcaa ctcgcgcacg cgcgccatct ccggcccctg
     3421 agtcagggcg ctgtcaacat tctgcggcgc tttatcaatc ttcagctcat actcttctcc
     3481 gatgcgttgt gctgcaacca tcgggtagaa accacgctgt tgcatgagtt gatgcaaaat
     3541 ctctttcgct tcagcttcac gtccgcgttc cagcaataaa tccgcctgcc agtaacgcca
     3601 ttcatctttc tcttttgctt ccatcggcag acgcgccagc caggtattca ggccgcgacg
     3661 atcgccggtg ccaagtgcca ttcgcacacg gcgttcaatc aacgacgtgg attgcgagcg
     3721 cataatggcg tcatcgcgcc atttcgcctg ctcgtcggtg acatcgttgc ccatcaaacg
     3781 ccaggcgacg atatcgcgca gctcctgaat atgatcttca ttaagctgct gcgcctgggc
     3841 aagcgacggg atcatcagcc gtgcattttc agcatcctgc cgcgccacac tggcaaacgc
     3901 caccgccgcc atttgacggg taaaatcggt cgctccggtt gtacgcgcga aggtcagtac
     3961 cgtattaggg ttgttcgcca gtgaaatgat tgccgaggcg atagtctggt aatcggcagg
     4021 catctgccct gccagcactg ttaccaggcc agtgttaccc gctttcatcg ccagacggat
     4081 acgctctaaa taagccagcg gatcttgttt acctgacgca cgccagacgc taaataactt
     4141 gtcacaggcg ttaggctggc tcttgccggt tagccacagc tctttcgccc cttgccaggc
     4201 ttcttcactc tgcccggtgt tccatttcgc atagtagtaa ttacattgcg cttcggtagt
     4261 tccgggcttt tccgggctaa aggctaacaa gccacgccag tcttcacgcc gcgccagttc
     4321 attgacgaaa cgagattgca gcgtgcgagc gggaggaagc gtggggttag cgcgaacaaa
     4381 gttagtgacc gtcaccgccg gttgattcat cagatcatcg gtgatctggc ggtattccag
     4441 gtagggataa agcggataat ccttcagtcc aggcatcatt tgttcgacca catccatttg
     4501 tcgattatcc caggcctgct tgatttgcgc gtaacggcta cgctgctcat ccagtgagtc
     4561 ggctcgcgcc acgctgctga ccgtcagcag acagacaccg gcagccaaca gccgccaggt
     4621 aacttgtttg gctttttcca caagcgcatc ctctaagtgt aaatacatca atgcagcgtc
     4681 atgccgcgta atagatttat gctaaccagt cattgccgtt tacgccacgt tacggacact
     4741 tttttacttt tactgcgaag gtgatcgacc aactgggatt aggcggggaa aaaggctaca
     4801 cttcgccttt gaaaactcat catcacgata aacatagagg cgaagtccaa cgtggctcaa
     4861 ttcgtttata ccatgcatcg tgtcggcaaa gttgttccgc cgaaacgtca tattttgaaa
     4921 aacatctctc tgagtttctt ccctggggca aaaattggtg tcctgggtct gaacggcgcg
     4981 ggtaagtcta ccctgctgcg cattatggcg ggcattgata aagacatcga aggtgaagcg
     5041 cgtccgcagc cagacatcaa gattggttac ctgccgcagg aaccgcagct gaacccggaa
     5101 cacaccgtgc gtgagtccat tgaagaagcg gtttctgaag tggttaacgc cctgaaacgc
     5161 ctggatgaag tgtatgcgct gtacgccgat ccggatgccg attttgacaa gctggccgct
     5221 gaacaaggcc gtctggaaga gatcattcag gctcacgacg gtcataacct gaacgtacag
     5281 ctggagcgtg cggcggatgc gctacgtctg ccggactggg acgcgaaaat cgctaacctc
     5341 tccggtggtg agcgtcgtcg cgtagcgttg tgccgcctgc tgctggaaaa accagacatg
     5401 ctgctgctcg acgaaccgac caaccacctg gatgccgaat ccgtggcctg gctggaacgc
     5461 ttcctgcacg acttcgaggg caccgtggtg gcgattaccc acgaccgtta cttcctcgat
     5521 aacgttgcag gctggatcct cgaacttgac cgcggtgaag gtattccgtg ggaaggcaac
     5581 tactcctcct ggctggagca gaaagatcag cgcctggcgc aggaagcttc acaagaagcg
     5641 gcgcgtcgta agtcgatcga gaaagagctg gagtgggtac gtcagggaac taaaggccgc
     5701 cagtcgaaag gtaaagcacg tctggcacgc tttgaagagc tgaacagcac cgaatatcag
     5761 aaacgtaacg aaaccaacga actgtttatt ccacctggac cgcgtctggg cgataaagtg
     5821 ctggaagtca gcaacctgcg taaatcctac ggtgatcgcc tgctgattga tgacctgagc
     5881 ttctcgatcc cgaaaggggc aatcgtcggg atcatcggtc cgaacggcgc gggtaaatcg
     5941 accctgttcc gtatgatctc tggtcaggaa cagccggaca gcggcaccat cactttaggt
     6001 gaaacggtga aactggcatc ggttgatcag ttccgtgact caatggataa cagcaaaacc
     6061 gtttgggaag aagtttccgg cgggctggat attatgaaga tcggcaacac cgagatgcca
     6121 agccgcgcct acgttggccg ctttaacttt aaaggggttg atcagggtaa acgcgttggt
     6181 gaactttccg gtggtgagcg cggtcgtctg catctggcga agctgctgca ggttggcggc
     6241 aacatgctgc tgctcgacga accaaccaac gacctggata tcgaaaccct gcgcgcgctg
     6301 gaaaacgccc tgctggagtt cccgggctgt gcgatggtta tctcgcacga ccgttggttc
     6361 ctcgaccgta tcgccacgca catcctggac taccaggatg aaggtaaagt tgagttcttc
     6421 gaaggtaact ttactgagta cgaagagtac aagaaacgca cgctgggcgc agacgcactg
     6481 gagccgaagc gtatcaagta caagcgtatt gcgaagtaat gcgtaaaatg ccggatgcgg
     6541 cgtgaacgcc ttatccggcc tacaaaccaa gaaatttcaa tacgttgtga atctccaggc
     6601 ctgataagac gcggtaagcg tcgcatcagg cattgagcac cgaatgccgg atacggcgtg
     6661 aacgcctttt ccggcctaca acagccttag tatccgcaat ttcgtagcgg gtcacccgtg
     6721 caggtttttt caaaccttgt ttaatctcga cagcctcttc cagaaaggtt ttcaactcat
     6781 caaaaaactt attttatacc tcgcctttga gccgtttcat cgcggttatc tctgctcccc
     6841 catcatctcc cgcaccagct ccacgcagcg caggaaacgg ctgtcataat cgtcctcttc
     6901 aacccgcacg aattcgatat tgttctcttc cagcatctcc accagcaagt tctggaactc
     6961 tttgcgatcc accgaactgc cgaggctgcg taaaccatcc gccacccacg gcgtgttgtt
     7021 ctccagcagg atcaccagat cgaaacggta ttcatcaatc aacgcctgca cgaacggatg
     7081 ctcacgccct tcgtactttt tgcagaacgc ctgggtagtg acaaaatcag tgtcaataaa
     7141 cgccacttta ttggcgtatt tcactgcaaa atcaatgtat tgtgcgtggc ccagcgcgat
     7201 tttatcgtag tcggaatact gcaatgcgat ctcatcaccg ccgaggtgtg aaaagacata
     7261 atcgcgacca tattcccacg cactggtggt attgaagata ttggcaagtt tgtttaccag
     7321 ggtggattta ccgctcgact cgccgccaag gatcgccacg gtacgcacaa agaacggctt
     7381 cacttccgta ggaatatatt cccagtagcg gaacgggttt tcgcggatct gcgcaccgct
     7441 gatactcata aaggtacgtt tcggatcgac cagtaccgtc tcgatcccca gatgttccat
     7501 atactgtggc gcatcggctt cttccgaggt gtagatcagg tccggctgga tccccttttc
     7561 agccataaac tttttgatgc cgttgctcca cacatcccag ccatgcggat acggctccat
     7621 gccctcttcg ttgaaagcat gaatgcgaat atttttctga tatttaaaag tttgcaataa
     7681 ccagcgcaga cgatccggca ccgtgggctg ctgcgacatg gcgctgtctt caaacaacgc
     7741 gcggtcgcgg gtatcgtcaa aacccataat gatatgcagc tcgtcaacct ggctacaggc
     7801 gcgctggata aggtagatat gtccggtatg cagcgggtag aacttaccga atacgacacc
     7861 aatggttttc ttctgccggg gaaattcaag ccccaaaaaa cggtgcaacg cctccagctt
     7921 ttgcgcgctg gggcttttaa ttttggcatt cagtaactgg cttaaatacc ctttggtcat
     7981 accgctggca tcagctacct gctgtaacgt gcagccctgt tgcttgatgg cagttttcag
     8041 gtaatcaaat gacgacatat ctccctccgt atctctcatt ataagtcgtc gaacacgcta
     8101 agcgcgtcgg agagtttttt aacgccaaaa atctgcatcc cttccggcgc ttttttcggc
     8161 acgttagccg ccggaacaat cgcccggcga aaaccgtgtt tcgccgcttc agagattcgc
     8221 tcctgaccgc tgggcaccgg gcggatctcc cctgccagcc cgacttcacc aaacaccacc
     8281 agatcctgcg gtagcggtct gtcacgcagg ctggaaacca tcgccagcag taacgctaag
     8341 tcggcactgg tttcggttac cttcacacca ccgaccacgt tcacaaatac atcctgatcg
     8401 gccatttgca gaccaccgtg acggtgcaag acagccagca ggattgccag acggttttgc
     8461 tccagaccta ccgccacgcg gcgcggattc gccatcatcg agtgatcgac cagcgcctga
     8521 atctccacca gcaacggacg cgttccttcc cacaccacca tcacggagct gccggaggtc
     8581 acttcatctc cgcgacttaa gaaaattgcc gaagggttgc tgacttcacg cagcccctgt
     8641 tcggtcatcg cgaagacgcc cagctcattc accgcgccga agcggttttt atggctgcgc
     8701 aaggtgcgaa aacgggagtc ggcatcgcca tccaaaagca ccgaacagtc gatacagtgt
     8761 tccagcactt tagggcccgc cagcgagcca tctttggtta cgtgtccaac catgacaatc
     8821 gccacaccgc gcgttttggc gaagcgtgtc aaataagccg ccgtttcacg cacctgcgcc
     8881 acgctaccag gcgatgactg tacatccgcc atatgcatca cctggatgga gtcaattacc
     8941 atcagcttcg gttgctcttc ttcagcaatc aggcagatct gctcgatgct ggtttccgac
     9001 aacatattga gattgtcagt cggcaggcca aggcgatgag cgcgcattgc cacctgttgc
     9061 agcgactctt cgccggtgac atacagcgtt ttcatctgct gggccagttt gcacagcgtt
     9121 tgcagcaaca gcgtggattt ccccgcgcct gggttaccgc caatcagaat ggcacttccc
     9181 ggtaccacgc cgccgcctaa tacgcggtcg aactctttaa atccggtgga aaaacgcggc
     9241 agctcttcaa ggctgatgtc ggagagtttc tggacttttg ccaccccggc gctaccggca
     9301 tagccactga gacgctcgtt acgcgccacc gttggcgacg cagcaagacg cacctcggtg
     9361 atggtgttcc aggcatgaca ggcactgcac tgcccctgcc agcgcggata atcggccccg
     9421 cattcattac aaacaaaggc gcgttttgga gcttttgcca cggtgtacct cgttaatgct
     9481 gtgccgcccg caggatgacg ggcaggcaat tacttctgat tcaggctgcc agagaggatg
     9541 cagaataccc ccatcaggtc agcgtgacgg atggtgactt ccgtcttttc attcactttt
     9601 ggcttggcat ggtaggcaat ccccagccct gccgctttga tcatcggcag gtcattggct
     9661 ccatcgccaa tcgccacggt ctgcgccagc gggatttcat actcctgcgc gaggcgggtc
     9721 agagttttcg ctttgtactg cgcgtcaacg atgtcgccga tcacattgcc ggtaaattta
     9781 ccgtccatga tctccagttc attggctacc acggcggtca ggcgcagctt gtcgcgcagg
     9841 tattcagcaa agaaagtaaa gccgccggag gcaatcgcca ctttccagcc cagcgtttcc
     9901 agcttgagca ccagctgcgt taagcctggc atcagcggca gattttcacg cacctgttgc
     9961 agaatattgg catcagcgcc tttcagcgtc gccacgcggc tgcgcaggct ggcggtaaaa
    10021 tcgagttcgc cgcgcatcgc ccgttcggtt acctccgcca ccatctcgcc cgttccggcc
    10081 agtttggcaa tttcatcaat acattcaatc tggatggcgg tggaatccat gtccatcacc
    10141 agcaaacccg gcgtgcgcag gtgcgggatt ttccccagcg gcgcgacatc cagttgcgct
    10201 tcatgggcca ggcgcgtagc gcgcgcggtg agtgaacctg ccagacgaat cacctgataa
    10261 tcttccacgc accaggcggc aacaatcacc atcgccgcgc ccagtttgct ctggtattgg
    10321 gtcaggcgtt gtttatccag cccacgacca tacagcagcc agccgctacg acctgcgtgg
    10381 taatccagtg gcatcacttc atcaccactt aatgaaagag gcagacccgg ccataaagag
    10441 acatcttcag gcaggtcgca ccaggtaatg ttaggcatta aggctcctgt aaaatcgttc
    10501 gaagcaggga aaataacgca tgaggctacc ttgtatccat tgcttctggc aacattaagt
    10561 ctcaaatttt caaagggtgg aagatggctc gcacaaaact gaaattccgg ctgcatcggg
    10621 cagtgattgt cctgttctgt cttgccttgt tagtggcact gatgcagggg gcgtcatggt
    10681 ttagtcaaaa ccaccagcga cagcgtaatc cacagctgga agaactggcc cgcaccctgg
    10741 cgcgtcaggt gacgctgaac gttgcaccgc tgatgcgtac cgactcacca gatgaaaaac
    10801 gcattcaggc gatcctcgat cagttaacgg atgaaagccg catcctcgac gcgggtgtgt
    10861 atgacgaaca aggcgatctt atcgcccgtt caggcgaaag cgtcgaagtg cgcgaccggc
    10921 tggcgctcga cggtaaaaaa gcaggcggct attttaacca gcagattgtc gagccaattg
    10981 cgggtaaaaa cggaccgctc ggctatctgc gcctgacact cgacacccat acgctcgcca
    11041 ccgaagccca acaggtggat aacaccacta acattttacg cctgatgttg ctgctctcac
    11101 tggcaatcgg cgtagtgctg acccgcacgc tgctacaggg taaacgcacc cgctggcagc
    11161 aatcgccctt cctgttaacc gccagcaaac cggtgccgga agaggaagaa agcgagaaaa
    11221 aagagtgacc cattactaca agaaaggaaa tcgttatgtc cacattacgc ctgctcatct
    11281 ctgactctta cgacccgtgg tttaacctgg cggtggaaga gtgtattttt cgccaaatgc
    11341 ccgccacgca gcgcgttctg tttctctggc gcaatgccga cacggtagtg attggtcgcg
    11401 cgcagaaccc gtggaaagag tgtaataccc ggcggatgga agaagataac gtccgcctgg
    11461 cgcggcgcag tagcggtggc ggcgcggtgt tccacgatct cggcaatacc tgctttacct
    11521 ttatggctgg caagccggag tacgataaaa ccatctctac gtcgattgtg ctcaatgcgc
    11581 tgaacgcgct cggcgtcagc gccgaagcgt ccgggcgtaa cgatctggtg gtgaaaacag
    11641 tcgaaggcga ccgcaaagtc tcaggctcgg cctatcgcga aaccaaagat cgtggcttcc
    11701 accacggcac tttgctgctc aatgccgacc ttagccgcct ggcaaactat ctcaatccgg
    11761 ataaaaagaa actggcggcg aaaggcatta cctcagtgcg ttcccgcgtg accaacctca
    11821 ccgagctgct gccggggatc acccatgagc aggtttgcga ggtcataacc aaggcctttt
    11881 tcgcccatta tggcgagcgt gtagaagcgg aaatcatctc cccggacaaa acgccagact
    11941 tgccaaactt cgccgaaacc tttgcccgtc agagtagctg ggaatggaac ttcggtcagg
    12001 ctccggcatt ctcgcatctg ctggatgaac gctttagctg gggcggcgtg gaactgcatt
    12061 tcgacgttga aaaaggccat atcacccgcg cccaggtgtt taccgacagc ctcaaccccg
    12121 cgccgctgga agccctcgcc gggcgactgc aaggctgcct gtaccgcgcg gatatgctgc
    12181 aacaagagtg cgaagcgctg ttggttgact tcccggacca ggaaaaagag ctacgggagt
    12241 tgtcgacgtg gatagcgggg gcggtaaggt aactcgctga ttaaaatacc aaaggaaggg
    12301 aatatgatcg atctggaaaa ccaggagcgg gagattatca atttaatgtt aagccagcgt
    12361 atttcctggc tagccgccgt gcgtatcagg cataaattat cgcttgctga agtaagtaaa
    12421 atgttaggta tctcgattaa ttctctaaaa cagatcgaaa agacggaacg gcttagctca
    12481 aacatcaaaa gcaaaatggc agaaatttat ggttgtccac cagagttgct gatatgccct
    12541 tcctgga
//
LOCUS       DAZKHF020000129        12450 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2201S31241
            SAMN43267800-rid25106413.denovo.130, whole genome shotgun sequence.
ACCESSION   DAZKHF020000129 DAZKHF020000000
VERSION     DAZKHF020000129.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267800
            Sequence Read Archive: SRR30307263
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 12450)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12450)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 12450)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350581.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350581.2
            Genome Coverage       :: 151x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:21:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,652
            CDSs (total)                      :: 5,557
            Genes (coding)                    :: 5,320
            CDSs (with protein)               :: 5,320
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 237
            CDSs (without protein)            :: 237
            Pseudo Genes (ambiguous residues) :: 0 of 237
            Pseudo Genes (frameshifted)       :: 102 of 237
            Pseudo Genes (incomplete)         :: 130 of 237
            Pseudo Genes (internal stop)      :: 52 of 237
            Pseudo Genes (multiple problems)  :: 41 of 237
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12450
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267800-rid25106413.denovo.130"
                     /serotype="O 26:H11"
                     /isolate="2201S31241"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-01"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            complement(138..1562)
                     /gene="patD"
                     /locus_tag="ACHOSC_RS14615"
                     /old_locus_tag="pgaptmp_002923"
     CDS             complement(138..1562)
                     /gene="patD"
                     /locus_tag="ACHOSC_RS14615"
                     /old_locus_tag="pgaptmp_002923"
                     /EC_number="1.2.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415961.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019145 - aminobutyraldehyde dehydrogenase
                     (NAD+) activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0009447 - putrescine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminobutyraldehyde dehydrogenase"
                     /protein_id="HGU4548827.1"
                     /translation="MQHKLLINGELVSGEGEKQPVYNPATGDVLLEIAEASAEQVDAA
                     VRAADAAFAEWGQTTPKVRAECLLKLADVIEENGQVFAELESRNCGKPLHSAFNDEIP
                     AIVDVFRFFAGAARCLNGLAAGEYLEGHTSMIRRDPLGVVASIAPWNYPLMMAAWKLA
                     PALAAGNCVVLKPSEITPLTALKLAELAKDIFPAGVINVLFGRGKTVGDPLTGHPKVR
                     MVSLTGSIATGEHIISHTASSIKRTHMELGGKAPVIVFDDADIEAVVEGVRTFGYYNA
                     GQDCTAACRIYAQKGIYDTLVEKLGAAVATLKSGAPDDESTELGPLSSLAHLERVSKA
                     VEEAKATGHIKVITGGEKRKGNGYYYAPTLLAGALQDDAIVQKEVFGPVVSVTPFDNE
                     EQVVNWANDSQYGLASSVWTKDVGRAHRVSARLQYGCTWVNTHFMLVSEMPHGGQKLS
                     GYGKDMSLYGLEDYTVVRHVMVKH"
     gene            complement(1584..2378)
                     /gene="ydcV"
                     /locus_tag="ACHOSC_RS14620"
                     /old_locus_tag="pgaptmp_002924"
     CDS             complement(1584..2378)
                     /gene="ydcV"
                     /locus_tag="ACHOSC_RS14620"
                     /old_locus_tag="pgaptmp_002924"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HGU4548828.1"
                     /translation="MHSERAPFFLKLAAWGGVVFLHFPILIIAAYAFNTEDAAFSFPP
                     QGLTLRWFSVAAQRSDILDAVTLSLKVAALATLMALVLGTLAAAALWRRDFFGKNAIS
                     LLLLLPIALPGIVTGLALLTAFKTINLEPGFFTIVVGHATFCVVVVFNNVIARFRRTS
                     WSLVEASMDLGANGWQTFRYVVLPNLSSALLAGGMLAFALSFDEIIVTTFTAGHERTL
                     PLWLLNQLGRPRDVPVTNVVALLVMLVTTLPILGAWWLTREGDNGQ"
     gene            complement(2368..3309)
                     /gene="ydcU"
                     /locus_tag="ACHOSC_RS14625"
                     /old_locus_tag="pgaptmp_002925"
     CDS             complement(2368..3309)
                     /gene="ydcU"
                     /locus_tag="ACHOSC_RS14625"
                     /old_locus_tag="pgaptmp_002925"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HGU4548829.1"
                     /translation="MAMNVLQSPSRPGLGKVSGFFWRNPRLGLFLLLLGPLMWFGIVY
                     FGSLLTLLWQGFYTFDDFTMSVTPELTLANIRALFNPANYDIILRTLTMAVAVTIASA
                     ILAFPMAWYMARYTSGKMKAFFYIAVMLPMWASYIVKAYAWTLLLAKDGVAQWFLQHL
                     GLEPLLTAFLTLPAVGGNTLSTSGLGRFLVFLYIWLPFMILPVQAALERLPPSLLQAS
                     ADLGARPRQTFRYVVLPLAIPGIAAGSIFTFSLTLGDFIVPQLVGPPGYFIGNMVYSQ
                     QGAIGNMPMAAAFTLVPIVLIALYLAFVKRLGAFDAL"
     gene            complement(3310..4323)
                     /gene="ydcT"
                     /locus_tag="ACHOSC_RS14630"
                     /old_locus_tag="pgaptmp_002926"
     CDS             complement(3310..4323)
                     /gene="ydcT"
                     /locus_tag="ACHOSC_RS14630"
                     /old_locus_tag="pgaptmp_002926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HGU4548830.1"
                     /translation="MTYAVEFDNVSRLYGDVRAVDGVSIAIKDGEFFSMLGPSGSGKT
                     TCLRLIAGFEQLSGGAISIFGKPASNLPPWERDVNTVFQDYALFPHMSILDNVAYGLM
                     VKGVNKKQRHAMAQEALEKVALGFVHQRKPSQLSGGQRQRVAIARALVNEPRVLLLDE
                     PLGALDLKLREQMQLELKKLQQSLGITFIFVTHDQGEALSMSDRVAVFNNGRIEQVDS
                     PRDLYMRPRTPFVAGFVGTSNVFDGLMAEKLCGMTGSFALRPEHIRLNTPGELQANGT
                     IQAVQYQGAATRFELKLNGGEKLLVSQANMTGEELPATLTPGQQVMVSWSRDVMVPLV
                     EER"
     gene            complement(4341..5486)
                     /gene="ydcS"
                     /locus_tag="ACHOSC_RS14635"
                     /old_locus_tag="pgaptmp_002927"
     CDS             complement(4341..5486)
                     /gene="ydcS"
                     /locus_tag="ACHOSC_RS14635"
                     /old_locus_tag="pgaptmp_002927"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative ABC transporter substrate-binding
                     protein YdcS"
                     /protein_id="HGU4548831.1"
                     /translation="MSKTFARSSLCALTMTIMTAHAAEPPTNLDKPEGRLDIIAWPGY
                     IERGQTDKQYDWVTQFEKETGCAVNVKTAATSDEMVSLMTKGGYDLVTASGDASLRLI
                     MGKRVQPINTALIPNWKTLDPRVVKGDWFNVGGKVYGTPYQWGPNLLMYNTKTFPTPP
                     DSWQVVFVEQNLPDGKSNKGRVQAYDGPIYIADAALFVKATQPQLGISDPYQLTEEQY
                     QAVLKVLRAQHSLIHRYWHDTTVQMSDFKNEGVVASSAWPYQANALKAEGQPVATVFP
                     KEGVTGWADTTMLHSEAKHPVCAYKWMNWSLTPKVQGDVAAWFGSLPVVPEGCKASPL
                     LGEKGCETNGFNYFDKIAFWKTPIAEGGKFVPYSRWTQDYIAIMGGR"
     gene            complement(5731..7136)
                     /gene="ydcR"
                     /locus_tag="ACHOSC_RS14640"
                     /old_locus_tag="pgaptmp_002928"
                     /pseudo
     CDS             complement(5731..7136)
                     /gene="ydcR"
                     /locus_tag="ACHOSC_RS14640"
                     /old_locus_tag="pgaptmp_002928"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415956.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
     gene            complement(7215..7631)
                     /gene="hicB"
                     /locus_tag="ACHOSC_RS14645"
                     /old_locus_tag="pgaptmp_002929"
     CDS             complement(7215..7631)
                     /gene="hicB"
                     /locus_tag="ACHOSC_RS14645"
                     /old_locus_tag="pgaptmp_002929"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415955.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system antitoxin HicB"
                     /protein_id="HGU4548832.1"
                     /translation="MRYPVTLTPAPEGGYMVSFVDIPEALTQGETVAEAMEAAKDALL
                     TAFDFYFEDNELIPLPSPLNSHDHFIEVPLSVASKVLLLNAFLQSEITQQELARRIGK
                     PKQEITRLFNLHHATKIDAVQLAAKALGKELSLVMV"
     gene            complement(7677..7853)
                     /gene="hicA"
                     /locus_tag="ACHOSC_RS14650"
                     /old_locus_tag="pgaptmp_002930"
     CDS             complement(7677..7853)
                     /gene="hicA"
                     /locus_tag="ACHOSC_RS14650"
                     /old_locus_tag="pgaptmp_002930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415954.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system mRNA interferase
                     toxin HicA"
                     /protein_id="HGU4548833.1"
                     /translation="MKQSEFRRWLESQGVDVANGSNHLKLRFHGRRSVMPRHPCDEIK
                     EPLRKAILKQLGLS"
     gene            8075..8305
                     /gene="yncJ"
                     /locus_tag="ACHOSC_RS14655"
                     /old_locus_tag="pgaptmp_002931"
     CDS             8075..8305
                     /gene="yncJ"
                     /locus_tag="ACHOSC_RS14655"
                     /old_locus_tag="pgaptmp_002931"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YncJ family protein"
                     /protein_id="HGU4548834.1"
                     /translation="MFTKALSVVLLTCALFSGQLMAGHKGHEFVWVKNVDHQLRHEAD
                     SDELRAVAEESAEGLREHFYWQKSRKPEAGQR"
     gene            complement(8397..10358)
                     /gene="rlhA"
                     /locus_tag="ACHOSC_RS14660"
                     /old_locus_tag="pgaptmp_002932"
     CDS             complement(8397..10358)
                     /gene="rlhA"
                     /locus_tag="ACHOSC_RS14660"
                     /old_locus_tag="pgaptmp_002932"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415952.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA 5-hydroxycytidine C2501 synthase"
                     /protein_id="HGU4548835.1"
                     /translation="MTVSSHRLELLSPARDAAIAREAILHGADAVYIGGPGFGARHNA
                     SNSLKDIAELVPFAHRYGAKIFVTLNTILHDDELEPAQRLITDLYQTGVDALIVQDMG
                     ILELDIPPIELHASTQCDIRTVEKAKFLSDVGFTQIVLARELNLEQIRAIHQATDATI
                     EFFIHGALCVAYSGQCYISHAQTGRSANRGDCSQACRLPYTLKDDQGRVVSYEKHLLS
                     MKDNDQTANLGALIDAGVRSFKIEGRYKDMSYVKNITAHYRQMLDAIIEERGDLARAS
                     SGRTEHFFVPSTEKTFHRGSTDYFVNARKGDIGAFDSPKFIGLPVGEVLKVAKDHLDV
                     AVTEPLANGDGLNVMIKREVVGFRANTVEKTGENQYRVWPNEMPADLHKIRPHHPLNR
                     NLDHNWQQALTKTSSERRVAVDIELGGWQEQLILTLTSEEGVSITHTLDGQFDEANNA
                     EKAMNNLKDGLAKLGQTLYYARDVQINLPGALFVPNSLLNQFRREAADMLDAARLASY
                     QRGSRKPVADPAPVYPQTHLSFFANVYNQKAREFYHRYGVQLIDAAYEAHEEKGEVPV
                     MITKHCLRFAFNLCPKQAKGNIKSWKATPMQLVNGDEVLTLKFDCRPCEMHVIGKIKN
                     HILKMPLPGSVVASVSPDELLKTLPKRKG"
     gene            complement(10431..10967)
                     /gene="sutR"
                     /locus_tag="ACHOSC_RS14665"
                     /old_locus_tag="pgaptmp_002933"
     CDS             complement(10431..10967)
                     /gene="sutR"
                     /locus_tag="ACHOSC_RS14665"
                     /old_locus_tag="pgaptmp_002933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator SutR"
                     /protein_id="HGU4548836.1"
                     /translation="MENLARFLSTTLKQLRQQRGWSLSRLAEATGVSKAMLGQIERNE
                     SSPTVATLWKIATGLNVPFSTFISPPQSATPSVYDPQQQAMVITSLFPYDPQLCFEHF
                     SIQMASGAISESTPHEKGVIEHVVVIDGQLDLCVDGEWQTLNCGEGVRFAADVTHIYR
                     NGGEQTVHFHSLIHYPRS"
     gene            11020..12234
                     /gene="ydcO"
                     /locus_tag="ACHOSC_RS14670"
                     /old_locus_tag="pgaptmp_002934"
     CDS             11020..12234
                     /gene="ydcO"
                     /locus_tag="ACHOSC_RS14670"
                     /old_locus_tag="pgaptmp_002934"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001748359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BenE family transporter YdcO"
                     /protein_id="HGU4548837.1"
                     /translation="MLRCLYAITHEVTMRLFSIPPPTLLAGFLAVLIGYASSAAIIWQ
                     AAIVAGATTAQISGWMTALGLAMGVSTLTLTLWYRVPVLTAWSTPGAALLVTGLQGLT
                     LNEAIGVFIVTNALIVLCGITGLFARLMRIIPHSLAAAMLAGILLRFGLQAFASLDGQ
                     FTLCGSMLLVWLATKAVAPRYAVIAAMIIGIVIVIAQGDVVTTDVVFKPVLPTYITPD
                     FSFAHSLSVALPLFLVTMASQNAPGIAAMKAAGYSAPVSPLIVFTGLLALVFSPFGVY
                     SVGIAAITAAICQSPEAHPDKDQRWLAAAVAGIFYLIAGLFGSAITGMMAALPVSWIQ
                     MLAGLALLSTISGSLYQALHNERERDAAVVAFLVTASGLTLVGIGSAFWGLIAGGVCY
                     VVLNLIADRNRY"
     gene            complement(12274..>12450)
                     /locus_tag="ACHOSC_RS14675"
                     /old_locus_tag="pgaptmp_002935"
     CDS             complement(12274..>12450)
                     /locus_tag="ACHOSC_RS14675"
                     /old_locus_tag="pgaptmp_002935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415949.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HGU4548838.1"
                     /translation="RLSQSKFRCQVCGYTANADVNGARNILAAGHAVLACGEMVQSGR
                     PLKQEPTEMIQATA"
BASE COUNT         3045 a         3343 c         3053 g         3009 t
ORIGIN      
        1 aggatgaaaa atcgccatca aacatttagc ctaattttta cgctagttat taatgtaatc
       61 attctaatac ttaaccgcca ttaaactcag aacctaaaac gatagtggcc gtcctataac
      121 agccactaac atctaactta atgtttaacc atgacgtggc ggacgacggt gtaatcctcc
      181 agcccataaa gtgacatatc cttgccgtaa ccagaaagtt tctgcccacc gtgcggcatt
      241 tcacttacca gcatgaaatg ggtattgacc caggtacaac catattgcag ccgtgcgctg
      301 acgcgatgcg ccctgcccac atctttcgtc cataccgaag atgcaagtcc gtactggctg
      361 tcattcgccc agttcaccac ctgttcttcg ttgtcgaagg gcgtaacact cactactgga
      421 ccaaatacct ctttttgcac gatggcatcg tcctgtaatg cgccagccag cagcgtcggc
      481 gcatagtaat agccattacc cttgcgcttt tcaccgccag tgatcacttt gatgtgccct
      541 gtcgctttcg cctcttctac tgccttgctg acgcgttcga gatgcgccag cgagcttaaa
      601 ggtccaagct ccgtagactc gtcatccggc gcaccagatt ttaacgttgc caccgcagca
      661 cccagttttt ccaccagcgt atcgtaaatg cctttttgcg cgtagatccg acaagccgca
      721 gtacaatcct gtccagcatt gtaatagcca aatgtacgta caccttcgac cactgcttca
      781 atatccgcat catcaaaaac aatcactggc gctttgccac caagttccat atgagtacgc
      841 ttaatggacg acgcggtatg gctgatgatg tgctcgccgg tggcgataga gcccgtcagc
      901 gacaccatcc gcactttggg atgaccggtc agcggatcac ccaccgtttt gcctctgcca
      961 aacagtacgt taatcacgcc tgccgggaag atatctttcg ccagctctgc caacttcaac
     1021 gcggtcagcg gggtaatttc tgatggttta agcactacgc agttccctgc cgccagcgcc
     1081 ggagccagtt tccacgctgc catcatcagc ggataattcc acggtgcgat agaagccacg
     1141 acccccaacg gatcgcgacg gatcatcgaa gtatgacctt caagatattc acctgccgcc
     1201 agaccattca gacagcgcgc cgcacccgcg aaaaagcgaa aaacatcgac aatcgccggg
     1261 atttcatcat tgaacgcact atgcagcggt ttgccacaat tacgggactc cagttcggca
     1321 aaaacctgac cattttcttc gataacatca gccagtttca gcagacattc cgcacgcact
     1381 ttcggcgtgg tttgccccca ttcggcaaat gctgcatctg ccgcgcgcac agcagcatcg
     1441 acctgctctg cggatgcctc ggcaatctcc agtaaaacgt cccccgttgc cggattatag
     1501 acaggctgtt tttccccttc gccgctaacc agttctccgt taatcagtaa cttatgttgc
     1561 atagcatatt cctgtatcag tggttattga ccattgtcgc cttcgcgggt tagccaccag
     1621 gcccccagga tcggcaaggt tgttaccaac ataaccagca gtgccaccac gttagttacc
     1681 ggtacatcac gcggtcgccc aagctgattg agcaaccaca acggtaacgt tcgttcatga
     1741 cctgccgtaa aagtcgtaac gatgatttca tcgaacgaca aggcaaacgc cagcattcct
     1801 cctgccagta gcgccgaact gagattcggc aacactacgt agcggaaggt ttgccagcca
     1861 ttggccccaa gatccattga cgcctcaacc agactccacg aggtgcggcg aaaacgggcg
     1921 atgacattgt taaacaccac cactacgcaa aaagtcgcat gaccgaccac gatggtgaaa
     1981 aatcccggct ccagattgat ggttttaaaa gcggttaata gcgccagacc agtgacaatg
     2041 cccggcagcg caatgggcag cagcagtaac agcgaaatgg cgtttttgcc gaaaaagtct
     2101 cgtcgccaca gcgcggcagc tgccagcgtc cctaacacca gcgccattaa tgtcgccagc
     2161 gccgccactt taagtgacag tgtcacggca tcaagaatat cactacgctg tgctgccacg
     2221 ctaaaccagc gcagcgtcag gccctgcggt ggaaaactaa acgccgcatc ttcagtgtta
     2281 aacgcataag cggcgataat caggatggga aaatgtagga aaaccacgcc gccccaggcc
     2341 gccagtttga ggaaaaacgg tgcgcgttca gagtgcatcg aacgctccca gacgtttcac
     2401 gaacgccagg tacagtgcga tgagaacaat cggcaccagg gtgaatgccg ccgccatcgg
     2461 catattgcca atcgccccct gctgggaata aaccatattg ccgataaaat atcctggagg
     2521 accaaccagc tgcgggacga taaaatcgcc cagtgtgagt gagaaggtaa agatagagcc
     2581 agcggcaata cccgggattg ccagcggcag caccacatag cgaaaggttt gtcgtggacg
     2641 tgcgccgaga tcagccgacg cctgcaacaa tgacggcggc aaacgctcaa gcgccgcctg
     2701 aacgggcagg atcatgaacg gcaaccagat atagagaaac accagaaagc gccccagccc
     2761 ggaagttgac agcgtatttc cgcccaccgc aggtaatgta aggaacgcag tcagcagtgg
     2821 ttccagccca agatgttgta aaaaccactg agccacgcca tcttttgcca gcaataacgt
     2881 ccaggcatag gctttaacaa tgtagctcgc ccacatcggc aacattaccg caatataaaa
     2941 aaaagctttc attttcccgc tggtatagcg cgccatatac cacgccattg gaaaagccag
     3001 aatggcgctg gcgatagtga ctgccacagc catagtcagc gtgcggagaa tgatgtcgta
     3061 attcgccgga ttaaacagcg cacggatatt cgccagcgtc agttccggcg ttaccgacat
     3121 ggtgaaatcg tcaaaagtat aaaatccctg ccataacagt gtcagcagcg agccgaaata
     3181 gacaatgcca aaccacatta gcgggccaag cagcagtaaa aacagcccca gcctcggatt
     3241 acgccagaag aaaccggaca ccttacccag acctggacgt gaaggtgatt gcaatacatt
     3301 catcgccatt cacctctcct caaccagcgg caccatcaca tcacgcgacc aggaaaccat
     3361 cacctgttgt ccgggcgtga gcgtggcagg cagttcttcg cctgtcatat tggcctgact
     3421 cacaagcagt ttttcaccgc cgttcaattt cagttcaaaa cgagttgccg cgccctgata
     3481 ttgcaccgcc tggatcgtgc cattggcctg cagttcacca ggggtgttga ggcggatatg
     3541 ttccggtcgc agggcgaagc ttcccgtcat gccacaaagt ttctctgcca tcagtccatc
     3601 aaaaacgttg gatgtcccga cgaatccggc aacaaacggc gtgcgcgggc gcatatagag
     3661 atcgcgcggg gaatcgacct gctcaatgcg tccattattg aaaaccgcca cacgatcgga
     3721 catcgataac gcttcgccct gatcgtgggt aacgaagata aaagtgatac cgagagactg
     3781 ttgcagtttt ttcagttcca gctgcatctg ctcacgcaat ttgagatcca gtgcgccgag
     3841 cggttcatcc aacagcaata cgcgcggttc attcaccaat gctctggcga tagcaacccg
     3901 ctggcgctga ccaccagaaa gttgtgacgg tttacgttga tgtacaaacc ccaacgccac
     3961 tttctccagc gcctcttgcg ccattgcgtg ccgctgcttt ttattcacgc ctttgaccat
     4021 cagcccatag gcgacattgt caagaatcga catatgcgga aatagcgcgt agtcctgaaa
     4081 gacagtattc acgtcccgct cccacggtgg cagattgctg gcgggtttac caaagataga
     4141 gatagccccg ccggaaagct gttcgaagcc agcaatcagg cgcaggcagg tggttttgcc
     4201 ggagccggac ggccccagca tagagaagaa ctcaccatct tttatcgcaa tactgacgcc
     4261 atctactgcg cgcacgtcac cgtacaaccg cgagacgttg tcaaactcca ctgcgtacgt
     4321 cataaagcac cccagcgaag ttagcgaccg cccataatgg caatgtaatc ctgcgtccag
     4381 cgactgtagg gaacaaactt gcccccttct gctataggcg ttttccagaa ggcgattttg
     4441 tcgaaatagt taaaaccgtt ggtttcacaa cctttttcgc ctaataacgg actggcttta
     4501 cacccttccg gcactaccgg taacgagcca aaccaggccg ccacatcgcc ctgcactttt
     4561 ggcgttaatg accagttcat ccatttgtag gcgcaaaccg gatgtttcgc ttcgctatgc
     4621 agcatggtgg tatcagccca accggtaaca ccctccttcg ggaaaacggt agcaacaggc
     4681 tggccttcgg ctttcagggc gttggcctga tagggccagg cactggaagc aaccacaccc
     4741 tcgtttttga aatcgctcat ttgcacggta gtgtcatgcc agtagcgatg gatcaaactg
     4801 tgttgagcgc gcagcacttt cagcaccgcc tggtactgtt cttcggttag ttgatacgga
     4861 tcgctgatgc ccaactgcgg ctgagtggct ttaacgaaca acgcagcgtc cgcaatgtag
     4921 atagggccat cataagcctg aacgcggcct ttattgctct tgccgtccgg cagattttgc
     4981 tcaacaaaaa ccacttgcca gctatccggc ggcgtcggga aggttttagt gttgtacatc
     5041 agcaggttcg gcccccattg gtaaggtgtg ccgtaaactt tgccgccaac attaaaccag
     5101 tcgcctttaa ccacgcgcgg atcgagcgtt ttccagttgg gaatcaaggc ggtattaatc
     5161 ggctgcacgc gtttgcccat aatcaaacgc agcgaggcat cgccggatgc cgtaaccaga
     5221 tcgtaacccc ctttggtcat cagactgacc atttcatcgg aagtcgcggc ggttttcaca
     5281 ttcaccgcgc agcctgtctc tttttcaaat tgcgttaccc agtcgtattg tttatcagtt
     5341 tgtccgcgtt cgatgtatcc cggccaggcg ataatatcca gtcgcccttc cggtttatct
     5401 aaattggtag gtggttcggc ggcgtgagcg gtcattattg tcatggtgag cgcacacagg
     5461 ctgctgcggg caaatgtctt gctcataagg tcttactcct gtcgtaatta attgttcagc
     5521 cgccgtgccg taaaaatatc tccttgttta accatagacg gcggcttgca ggcacacctt
     5581 cgcccctgag aaaatttaat caggttgtga gctaccgcgc attatgccgt ctgtaaggtt
     5641 atcttctgga gtatagacaa ggggttagga aatatgactg ctattcgaaa ttcatattac
     5701 ccaatctaag aggaattaaa ttaaacgcta ttacagccgt tcttgaataa gtttgcctaa
     5761 ttgttttacc gcctgttctt cacgttctcc ccactgccac gcggtattaa aacgaaaaaa
     5821 acgtgaccag ttttcaccgg tagaaaacat tttacccggc gcaatactga tatgatgcgt
     5881 cagtgccgcc aggcttaatt cgccggcatc taacggctcg gggagctcca gccagagaaa
     5941 ataaccgctg tcactatgat gaattttaac ttccgcaggc agataacgca gcagtgcctg
     6001 ccaggcacgt tgtttacgtt ccgcaagctg gcgacgcagg cgacgaagat gggcgtcgta
     6061 tcggcgcgtg gaaaggtaat ccaccagcgc aagctgcatc ggtgagctgg tggaaagcgt
     6121 actcatcaac tgcaagcgtt gaattttacg tgcatgtttt ccggcggcga cccaaccaat
     6181 acgaaaacca ggcaccagac atttcgaaaa cgaagagcaa tgcaaaacgc catcgtggcg
     6241 atcccacgct ttcgcaggca gcggtttttc ccgtccaaaa taaagttcgc tgtaaacgtc
     6301 atcttcaatc agcgttacgt tgtactgatt gagtaacgcc accagttgtg ctttttttgc
     6361 ggcgttaagg taaatccgag tggattctgg ctattagtca tcagccagca cgctttcacc
     6421 ggatactcct gcaacgccag ttccaacgcc tgaagatcta tcccttcttt aacatccgtc
     6481 gccaccgata acgctttcag ccgtagccgt tccagcgcct gcaacgcgcc atagaaacaa
     6541 ggattctcta ctatcaccca atcgcccggt tcagttaccg cttgcaaact gaggtttaat
     6601 gcctctaacg ccccggcagt aatgacaatt tcatcaggag aaatggtgat gccctgtaag
     6661 gcatagcgac gagcaatagc ctgacgcagt tctgcgtttc ctggcggtaa gttttcaatc
     6721 acgctcatcg ccgtggcggt tttgcttacc tgcgccagcg agcggtttag ttgttggagg
     6781 gggaaaagtc gcgggtcggg aaaggccgag gcaaacggaa cgaccgacgg atcgcgactg
     6841 gcctgcaaca tatcaaaaat ataagtgttg atatcgactg cttcatctcg agtgactgga
     6901 atgactggcg ctttcggcat ttttattgcc tgtggcgcaa cgtaataacc cgactgcggt
     6961 cgtgcgataa tatatccctg actttcgagc aactgatagg catggctgac agtcataaag
     7021 ctcatgcctg aaagcgccac ctggtcacgc aacgaaggca aacgatcgcc gggttgccag
     7081 atacccgacg caatctgctc gcgtaattgt tctgcaagct gctggtattt tttcattatc
     7141 gaatcgtaat tatgtgcaga tgattcggca gtctatatca gttgttaaaa atgacaactt
     7201 ttcgttaact gtaattaaac catcaccagc gataactctt tgccaagcgc ctttgccgca
     7261 agctggacgg cgtcgatttt tgtcgcatgg tgcaagttaa ataggcgagt aatctcctgt
     7321 ttaggtttgc caattcgcct ggctaactct tgctgagtga tttctgactg taaaaaagca
     7381 tttaacagca atactttaga ggcgacgctc aaaggtactt caataaagtg atcatgacta
     7441 tttaatggcg aaggtaacgg gataagctcg ttatcttcaa aataaaaatc aaatgcggtc
     7501 agtaaagcat cttttgccgc ttccatcgct tcagcgacag tttcgccctg cgtcaacgct
     7561 tcagggatat ccacaaaaga aaccatataa ccgccttccg gcgcgggtgt aagagtgacg
     7621 ggataacgca taatttcgac tgtccctcgc atgtcattaa cctttttaat tggcgattaa
     7681 ctcaaaccga gttgtttcag gattgcttta cgcaatggtt ctttaatctc atcgcagggg
     7741 tgacgcggca tgacactgcg cctcccatga aacctgagtt tcaaatggtt gctgccattc
     7801 gctacatcga cgccctgaga ttcgagccaa cgtctgaact cgctttgttt cacatcgcct
     7861 ccctgccgct gagtatatca acaaagtaaa cattagtgtt tacctccgtg tgtaatttgc
     7921 tcgaaaatag cactaaatcg ccgcaacggc agaatgccat gcaaaatcag gaagaaagac
     7981 tccagaattt ctttgcgcta atttgcaaaa ctgaataata acctcaagaa acgatcggca
     8041 tcatagcggc aaaaggtaag aaaaaggtgg caggatgttt acgaaggcgt tatcggttgt
     8101 cttattaacg tgtgcgctgt tctcaggaca actcatggca gggcacaaag gacatgaatt
     8161 tgtgtgggta aagaatgtgg atcatcagct gcgtcatgaa gcggacagcg atgaattgcg
     8221 tgctgtggcg gaagagtcgg cggaaggttt gcgcgagcat ttttactggc aaaaatcgcg
     8281 caaaccagaa gcgggacaac gttgagtcat ttgttgccgg atgcggcgag acgccttacc
     8341 cggcaggcaa tatcatgcag attcaatgag ttgtgagtaa ccagaaaact ggcgttttac
     8401 ccttttcgct taggcaatgt tttcagcagc tcatccggac ttacggatgc cactacgctt
     8461 cccggtaacg gcattttcag tatgtgattt ttgattttgc caatgacgtg catctcgcat
     8521 gggcggcaat caaactttag cgttaatact tcatcgccgt taaccagttg cattggcgtc
     8581 gccttccagc ttttgatatt gcctttcgcc tgtttcgggc acagattaaa ggcaaagcgc
     8641 agacaatgct tggtgatcat caccgggact tcgcccttct cttcatgtgc ttcatacgcc
     8701 gcgtcaatca gctgcacacc atagcgatga taaaattcac gcgctttctg gttgtatacg
     8761 ttcgcgaaga aactcagatg cgtttgcgga taaaccggcg caggatcagc aaccggttta
     8821 cggctgccgc gctggtaact ggcaagacgc gcagcatcca gcatgtcagc agcttcacgg
     8881 cggaactggt ttaacagact gtttggtaca aacagcgccc ccggcaaatt aatttgcaca
     8941 tcgcgggcgt aatagagggt ttgccccagt tttgccagac catccttcag attgttcatt
     9001 gctttttcgg cgttattggc ttcgtcgaac tgcccgtcca gcgtatgcgt gatgctgaca
     9061 ccctcttcac tggtgagggt cagaatcagt tgttcctgcc agccgcccag ttcaatgtct
     9121 accgccaccc gacgttcgct ggaggttttt gtcagtgcct gctgccagtt atgatcaaga
     9181 ttacggttta gtgggtgatg tggacgaatt ttgtgcaaat ctgctggcat ttcattgggc
     9241 cagacgcggt actgattttc tccggttttc tcgaccgtat tggcacgaaa accgacgact
     9301 tcacgtttaa tcatcacgtt caggccatca ccatttgcca gtggctcggt gacggcaaca
     9361 tcgagatgat ctttcgccac tttcaatact tcgcctaccg gcaggccgat aaatttcggc
     9421 gaatcgaacg cgccaatatc gcctttacgg gcattcacaa aataatctgt gctaccacgg
     9481 tggaaagtct tttccgtcga tggaacaaag aaatgttcag tacgacctga tgaagcgcgc
     9541 gccagatcgc cacgttcttc aataatggca tcaagcatct ggcgataatg ggcggtgata
     9601 ttcttcacgt agctcatatc tttgtaacgc ccttcaatct tgaaggagcg tacgccagca
     9661 tcaatcagcg cgccgaggtt ggcagtctga tcgttatctt tcatcgacag cagatgtttt
     9721 tcataggaaa ccacccgccc ctgatcgtct ttcaatgtgt atggcaaacg gcacgcctgc
     9781 gagcaatcac cacggttggc gctacgccct gtttgcgcat gagaaatgta gcactgaccc
     9841 gaataggcca cgcacagtgc cccatgaata aagaattcaa tggtcgcgtc cgtggcctgg
     9901 tgaatcgcgc ggatctgctc aagattcagc tctcgcgcca gcacaatctg cgtgaagcca
     9961 acatcagaga ggaacttcgc tttttctact gtacgaatgt cgcactgcgt actggcgtgc
    10021 agttcaatcg gcggaatatc aagttccaga atccccatat cctgaacaat cagcgcatcg
    10081 acaccggtct ggtagaggtc ggtaatcagc cgttgcgcgg gttccagctc atcatcatgc
    10141 aaaatggtgt taagcgtgac gaaaattttt gcaccataac gatgggcaaa cggcaccagc
    10201 tcggcaatat ctttcaggct attactggca ttatgacggg caccaaaacc agggccgccg
    10261 atataaacag catcggcacc gtgcaaaata gcttcgcggg caatggcggc atcgcgtgcc
    10321 gggcttaaca gttcaagtcg atgagaagat acggtcacat tcttatccag tagctgaaaa
    10381 atggcggcta ttttagccac aagcgcgacg ttgcgaaata gttttcccgc ttaactgcgc
    10441 gggtaatgga tgagggaatg aaaatgtacg gtttgctccc caccattacg atagatatgc
    10501 gtgacgtctg cagcaaatcg aacgccttcc ccacaattaa gggtctgcca ttccccatca
    10561 acgcacagat caagttgtcc atcaatgacg acgacatgtt cgattacccc cttctcatgg
    10621 ggcgtcgatt cgcttattgc accagatgcc atctgaatcg agaagtgttc gaagcagagt
    10681 tgtggatcat aaggaaacag cgaagtgatc accatcgcct gctgttgtgg atcataaact
    10741 gaaggcgtag cggactgcgg cggagaaata aatgttgaaa atggtacgtt caacccggtg
    10801 gcaattttcc ataatgtcgc gaccgtcgga ctggactcat tacgctcaat ctgccctaac
    10861 attgctttag aaacgcccgt cgcttctgcc agtcgcgaaa gactccagcc gcgctgctgg
    10921 cgtagttgtt taagtgtggt ggataaaaag cgagcgagat tttccatgcg actcctccgg
    10981 caaaacggaa gtttatcact tgtgcgttat aacggacaaa tgctacggtg cctgtacgct
    11041 ataacgcacg aggtgactat gcgtctgttt tctattcctc cacccacgct actggcgggg
    11101 tttctggcgg tattaattgg ctacgccagt tcagcggcaa taatctggca agcagcgatt
    11161 gtcgccggag ccaccactgc acaaatctct ggctggatga cggcgctggg gctggcaatg
    11221 ggcgtcagta cgctgactct gacattatgg tatcgcgtac ctgttctcac cgcatggtca
    11281 acgcctggcg cggctttgtt ggtcaccgga ttgcagggac taacacttaa cgaagccatc
    11341 ggcgttttta ttgtcaccaa cgcgttaata gtcctctgcg gcataacggg actctttgct
    11401 cgtctgatgc gcattattcc gcactcgctt gcggcggcaa tgcttgccgg gattttatta
    11461 cgctttggtt tacaggcgtt tgccagtctg gacggtcaat ttacgttgtg tggaagtatg
    11521 ttgctggtat ggctggcaac caaggccgtt gcgccgcgct atgcggtaat tgccgcgatg
    11581 attattggga tcgtgatcgt tatcgcgcaa ggtgacgttg tcacaactga tgttgtcttt
    11641 aaacccgttc tccccactta tattacccct gatttttcgt ttgctcacag cctgagcgtt
    11701 gcactccccc tttttctggt gacgatggca tcgcaaaacg caccgggtat cgcagcaatg
    11761 aaagcagccg ggtattcggc tcctgtttcg ccattaattg tatttactgg attgctggca
    11821 ctggtttttt cccctttcgg cgtttattcc gtcggtattg cggcaatcac cgcggctatt
    11881 tgccaaagcc cggaagcgca tccggataaa gatcaacgtt ggctggcagc tgccgttgca
    11941 ggcatttttt atttgatcgc aggtctgttt ggtagtgcta ttaccgggat gatggctgcc
    12001 ctgcccgtaa gttggatcca gatgctggca ggtctggcgc tgttaagtac cattagcggc
    12061 agtttgtatc aggcgctgca taatgagcgt gagcgcgacg cggcggtggt ggcatttctg
    12121 gtaacggcaa gtggattgac gctggtcggg attggttctg cgttctgggg attaattgcc
    12181 ggaggcgttt gttacgtggt gttgaattta atcgctgaca gaaaccgata ttgacatcct
    12241 ccacgccctg aaggacgagg atccctgcta cgttcaggct gtcgcctgaa tcatttcggt
    12301 gggttcctgc ttcaacgggc ggcctgactg caccatctct ccacaggcaa gaacggcgtg
    12361 ccccgccgct aaaatgttac gagcgccatt tacatcggcg ttcgctgtat atccacatac
    12421 ctggcatctg aatttacttt gtgacaggcg
//
LOCUS       DAZKHF020000130        12314 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2201S31241
            SAMN43267800-rid25106413.denovo.131, whole genome shotgun sequence.
ACCESSION   DAZKHF020000130 DAZKHF020000000
VERSION     DAZKHF020000130.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267800
            Sequence Read Archive: SRR30307263
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 12314)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12314)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 12314)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350581.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350581.2
            Genome Coverage       :: 151x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:21:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,652
            CDSs (total)                      :: 5,557
            Genes (coding)                    :: 5,320
            CDSs (with protein)               :: 5,320
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 237
            CDSs (without protein)            :: 237
            Pseudo Genes (ambiguous residues) :: 0 of 237
            Pseudo Genes (frameshifted)       :: 102 of 237
            Pseudo Genes (incomplete)         :: 130 of 237
            Pseudo Genes (internal stop)      :: 52 of 237
            Pseudo Genes (multiple problems)  :: 41 of 237
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12314
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267800-rid25106413.denovo.131"
                     /serotype="O 26:H11"
                     /isolate="2201S31241"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-01"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            3..1517
                     /gene="caiT"
                     /locus_tag="ACHOSC_RS14680"
                     /old_locus_tag="pgaptmp_002936"
     CDS             3..1517
                     /gene="caiT"
                     /locus_tag="ACHOSC_RS14680"
                     /old_locus_tag="pgaptmp_002936"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-carnitine/gamma-butyrobetaine antiporter"
                     /protein_id="HGU4548839.1"
                     /translation="MKNEKRKTGIEPKVFFPPLIIVGILCWLTVRDLDAANVVINAVF
                     SYVTNVWGWAFEWYMVVMLFGWFWLVFGPYAKKRLGNEPPEFSTASWIFMMFASCTSA
                     AVLFWGSIEIYYYISTPPFGLEPNSTGAKELGLAYSLFHWGPLPWATYSFLSVAFAYF
                     FFVRKMEVIRPSSTLVPLVGEKHAKGLFGTIVDNFYLVALIFAMGTSLGLATPLVTEC
                     MQWLFGIPHTLQLDAIIITCWIILNAICVACGLQKGVRIASDVRSYLSFLMLGWVFIV
                     SGASFIMNYFTDSVGMLLMYLPRMLFYTDPIAKGGFPQGWTVFYWAWWVIYAIQMSIF
                     LARISRGRTVRELCFGMVLGLTASTWILWTVLGSNTLLLIDKNIINIPNLIEQYGVAR
                     AIIETWAALPLSTATMWGFFILCFIATVTLVNACSYTLAMSTCREVRDGEEPPLLVRI
                     GWSILVGIIGIVLLALGGLKPIQTAIIAGGCPLFFVNIMVTLSFIKDAKQNWKD"
     gene            1548..2690
                     /gene="caiA"
                     /locus_tag="ACHOSC_RS14685"
                     /old_locus_tag="pgaptmp_002937"
     CDS             1548..2690
                     /gene="caiA"
                     /locus_tag="ACHOSC_RS14685"
                     /old_locus_tag="pgaptmp_002937"
                     /EC_number="1.3.8.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003995 - acyl-CoA dehydrogenase activity
                     [Evidence IEA];
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA];
                     GO_process: GO:0009437 - carnitine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="crotonobetainyl-CoA dehydrogenase"
                     /protein_id="HGU4548840.1"
                     /translation="MDFNLNDEQELFVAGIRELMASENWEAYFAECDRDSVYPERFVK
                     ALADMGIDSLLIPEEHGGLDAGFVTLAAVWMELGRLGAPTYVLYQLPGGFNTFLREGT
                     QEQIDKIMAFRGTGKQMWNSAITEPGAGSDVGSLKTTYTRRNGKIYLNGSKCFITSSA
                     YTPYIVVMARDGASPDKPVYTEWFVDMSKPGIKVTKLEKLGLRMDSCCEITFDDVELD
                     EKDMFGREGNGFNRVKEEFDHERFLVALTNYGTAMCAFEDAARYANQRVQFGEAIGRF
                     QLIQEKFAHMAIKLNSMKNMLYEAAWKADNGTITSGDAAMCKYFCANAAFEVVDSAMQ
                     VLGGVGIAGNHRISRFWRDLRVDRVSGGSDEMQILTLGRAVLKQYR"
     gene            2819..4036
                     /gene="caiB"
                     /locus_tag="ACHOSC_RS14690"
                     /old_locus_tag="pgaptmp_002938"
     CDS             2819..4036
                     /gene="caiB"
                     /locus_tag="ACHOSC_RS14690"
                     /old_locus_tag="pgaptmp_002938"
                     /EC_number="2.8.3.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_705994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016782 - transferase activity,
                     transferring sulphur-containing groups [Evidence IEA];
                     GO_process: GO:0009437 - carnitine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-carnitine CoA-transferase"
                     /protein_id="HGU4548841.1"
                     /translation="MDHLPMPKFGPLAGLRVVFSGIEIAGPFAGQMFAEWGAEVIWIE
                     NVAWADTIRVQPNYPQLSRRNLHALSLNIFKDEGREAFLKLMETTDIFIEASKGPAFA
                     RRGITDEVLWQHNPKLVIAHLSGFGQYGTEEYTNLPAYNTIAQAFSGYLIQNGDVDQP
                     MPAFPYTADYFSGLTATTAALAALHKVRETGKGESIDIAMYEVMLRMGQYFMMDYFNG
                     GEMCPRMSKGKDPYYAGCGLYKCADGYIVMELVGITQIEECFKDIGLAHLLGTPEIPE
                     GTQLIHRIECPYGPLVEEKLDAWLAAHTIAEVKERFAELNIACAKVLTVPELESNPQY
                     VARESITQWQTMDGRTCKGPNIMPKFKNNPGQIWRGMPSHGMDTAAILKNIGYSENDI
                     QELVSKGLAKVED"
     gene            4110..5663
                     /gene="caiC"
                     /locus_tag="ACHOSC_RS14695"
                     /old_locus_tag="pgaptmp_002939"
     CDS             4110..5663
                     /gene="caiC"
                     /locus_tag="ACHOSC_RS14695"
                     /old_locus_tag="pgaptmp_002939"
                     /EC_number="6.2.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308067.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016405 - CoA-ligase activity [Evidence
                     IEA];
                     GO_process: GO:0009437 - carnitine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="crotonobetaine/carnitine-CoA ligase"
                     /protein_id="HGU4548842.1"
                     /translation="MDIIGGQHLRQMWDDLADVYGHKTALICESSGGVVNRYSYLELN
                     QEINRTANLFYTLGIRKGDKVALHLDNCPEFIFCWFGLAKIGAIMVPINARLLREESA
                     WILQNSQACLLVTSAQFYPMYQQIQQEDATQLRHICLTDVALPADDGVSSFTQLKNQQ
                     PATLCYAPPLLTDDTAEILFTSGTTSRPKGVVITHYNLRFAGYYSAWQCALRDDDVYL
                     TVMPAFHIDCQCTAAMAAFSAGATFVLVEKYSARAFWGQVQKYRATITECIPMMIRTL
                     MVQPPSANDRQHRLREVMFYLNLSEQEKDAFCERFGVRLLTSYGMTETIVGIIGDRPG
                     DKRRWPSIGRAGFCYEAEIRDDHNRPLPAGEIGEICIKGVPGKTIFKEYFLNPKATAK
                     VLEADGWLHTGDTGYCDEEGFFYFVDRRCNMIKRGGENVSCVELENIIATHPKIQDIV
                     VVGIKDSIRDEAIKAFVVLNEGETLSEEEFFRFCEQNMAKFKVPSYLEIRKDLPRNCS
                     GKIIRKNLK"
     gene            5772..6557
                     /gene="caiD"
                     /locus_tag="ACHOSC_RS14700"
                     /old_locus_tag="pgaptmp_002940"
     CDS             5772..6557
                     /gene="caiD"
                     /locus_tag="ACHOSC_RS14700"
                     /old_locus_tag="pgaptmp_002940"
                     /EC_number="4.2.1.149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414578.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016836 - hydro-lyase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="crotonobetainyl-CoA hydratase"
                     /protein_id="HGU4548843.1"
                     /translation="MSESLHLTRNGSILEITLDRPKANAIDAKTSFEMGEVFLNFRDD
                     PQLRVAIITGAGEKFFSAGWDLKAAAEGEAPDADFGPGGFAGLTEIFNLDKPVIAAVN
                     GYAFGGGFELALAADFIVCADNASFALPEAKLGIVPDSGGVLRLPKILPPAIVNEMVM
                     TGRRMGAEEALRWGIVNRVVSQAELMDNARELAQQLVNSAPLAIAALKEIFRTTSEMP
                     VEEAYRYIRSGVLKHYPSVLHSEDAIEGPLAFAEKRDPVWKGR"
     gene            6563..7153
                     /gene="caiE"
                     /locus_tag="ACHOSC_RS14705"
                     /old_locus_tag="pgaptmp_002941"
     CDS             6563..7153
                     /gene="caiE"
                     /locus_tag="ACHOSC_RS14705"
                     /old_locus_tag="pgaptmp_002941"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308065.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carnitine operon protein CaiE"
                     /protein_id="HGU4548844.1"
                     /translation="MSYYAFEGLIPVVHPTAFVHPSAVLIGDVIVGAGVYIGPLASLR
                     GDYGRLIVQAGANIQDGCIMHGYCDTDTIVGENGHIGHGAILHGCVIGRDALVGMNSV
                     IMDGAVIGEESIVAAMSFVKAGFHGEKRQLLMGTPARAVRSVSDDELHWKRLNTKEYQ
                     DLVGRCHASLHETQPLRQMEENRPRLQGTTDVTPKR"
     gene            complement(7239..7634)
                     /gene="caiF"
                     /locus_tag="ACHOSC_RS14710"
                     /old_locus_tag="pgaptmp_002942"
     CDS             complement(7239..7634)
                     /gene="caiF"
                     /locus_tag="ACHOSC_RS14710"
                     /old_locus_tag="pgaptmp_002942"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414576.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carnitine metabolism transcriptional regulator
                     CaiF"
                     /protein_id="HGU4548845.1"
                     /translation="MCEGYVEKPLYLLIAEWMMAENRWVIAREISIHFDIEHSKAVNT
                     LTYILSEVAEISCEVKMIPNKLEGRGCQCQRLVKVVDIDEQIYARLRNNSRDKLVGVR
                     KTPRIPAVPLTELNREQKWQMMLSKSMRR"
     gene            complement(7895..11116)
                     /gene="carB"
                     /locus_tag="ACHOSC_RS14715"
                     /old_locus_tag="pgaptmp_002943"
     CDS             complement(7895..11116)
                     /gene="carB"
                     /locus_tag="ACHOSC_RS14715"
                     /old_locus_tag="pgaptmp_002943"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyl-phosphate synthase large subunit"
                     /protein_id="HGU4548846.1"
                     /translation="MPKRTDIKSILILGAGPIVIGQACEFDYSGAQACKALREEGYRV
                     ILVNSNPATIMTDPEMADATYIEPIHWEVVRKIIEKERPDAVLPTMGGQTALNCALEL
                     ERQGVLEEFGVTMIGATADAIDKAEDRRRFDVAMKKIGLETARSGIAHTMEEALAVAA
                     DVGFPCIIRPSFTMGGSGGGIAYNREEFEEICARGLDLSPTKELLIDESLIGWKEYEM
                     EVVRDKNDNCIIVCSIENFDAMGIHTGDSITVAPAQTLTDKEYQIMRNASMAVLREIG
                     VETGGSNVQFAVNPKNGRLIVIEMNPRVSRSSALASKATGFPIAKVAAKLAVGYTLDE
                     LMNDITGGRTPASFEPSIDYVVTKIPRFNFEKFAGANDRLTTQMKSVGEVMAIGRTQQ
                     ESLQKALRGLEVGATGFDPKVSLDDPEALTKIRRELKDAGAERIWYIADAFRAGLSVD
                     GVFNLTNIDRWFLVQIEELVRLEEKVAEVGITGLNAEFLRQLKRKGFADARLAKLAGV
                     REAEIRKLRDQYDLHPVYKRVDTCAAEFATDTAYMYSTYEEECEANPSTDREKIMVLG
                     GGPNRIGQGIEFDYCCVHASLALREDGYETIMVNCNPETVSTDYDTSDRLYFEPVTLE
                     DVLEIVRIEKPKGVIVQYGGQTPLKLARALEAAGVPVIGTSPDAIDRAEDRERFQHAV
                     ERLKLKQPANATVTAIEMAVEKAKEIGYPLVVRPSYVLGGRAMEIVYDEADLRRYFQT
                     AVSVSNDAPVLLDHFLDDAVEVDVDAICDGEMVLIGGIMEHIEQAGVHSGDSACSLPA
                     YTLSQEIQDVMRQQVQKLAFELQVRGLMNVQFAVKNNEVYLIEVNPRAARTVPFVSKA
                     TGVPLAKVAARVMAGKSLAEQGVTKEVIPPYYSVKEVVLPFNKFPGVDPLLGPEMRST
                     GEVMGVGRTFAEAFAKAQLGSNSTMKKHGRALLSVREGDKERVVDLAAKLLKQGFELD
                     ATHGTAIVLGEAGINPRLVNKVHEGRPHIQDRIKNGEYTYIINTTSGRRAIEDSRVIR
                     RSALQYKVHYDTTLNGGFATAMALNADATEKVISVQEMHAQIK"
     gene            complement(11134..12282)
                     /gene="carA"
                     /locus_tag="ACHOSC_RS14720"
                     /old_locus_tag="pgaptmp_002944"
     CDS             complement(11134..12282)
                     /gene="carA"
                     /locus_tag="ACHOSC_RS14720"
                     /old_locus_tag="pgaptmp_002944"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004088 - carbamoyl-phosphate synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006207 - 'de novo' pyrimidine nucleobase
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0006541 - glutamine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing carbamoyl-phosphate
                     synthase small subunit"
                     /protein_id="HGU4548847.1"
                     /translation="MIKSALLVLEDGTQFHGRAIGATGSAVGEVVFNTSMTGYQEILT
                     DPSYSRQIVTLTYPHIGNVGTNDADEESSQVHAQGLVIRDLPLIASNFRNTEDLSSYL
                     KRHNIVAIADIDTRKLTRLLREKGAQNGCIIAGDNPDAALALEKARAFPGLNGMDLAK
                     EVTTAEAYSWTQGSWTLTGGLPEAKKEDELPFHVVAYDFGAKRNILRMLVDRGCRLTI
                     VPAQTSAEDVLKMNPDGIFLSNGPGDPAPCDYAITAIQKFLETDIPVFGICLGHQLLA
                     LASGAKTVKMKFGHHGGNHPVKDVEKNVVMITAQNHGFAVDEATLPANLRVTHKSLFD
                     GTLQGIHRTDKPAFSFQGHPEASPGPHDAAPLFDHFIELIEQYRKTAK"
BASE COUNT         2828 a         3238 c         3294 g         2954 t
ORIGIN      
        1 tcatgaagaa tgaaaagaga aaaacgggaa tagaaccgaa ggttttcttt ccgccgttaa
       61 taatcgtcgg catactttgt tggcttacag tcagagatct ggatgcagcg aatgtcgtta
      121 ttaatgctgt attcagttac gtcaccaatg tatggggatg ggcatttgaa tggtatatgg
      181 tggtgatgct tttcggttgg ttctggctgg tgtttggccc gtatgccaaa aagcgtttag
      241 gtaacgaacc acctgaattt agtaccgcca gttggatctt tatgatgttc gcctcctgta
      301 cgtctgctgc cgtactgttc tggggatcga ttgagatcta ctactacatc tccaccccgc
      361 cgtttggctt agaaccgaac tcgacagggg cgaaagagtt ggggctggct tacagcttgt
      421 tccactgggg acctctgccg tgggccactt acagcttcct ttcagtcgcc ttcgcttact
      481 tcttctttgt ccgcaaaatg gaagtgattc gccccagctc gacactggtg ccgctggtag
      541 gtgaaaaaca cgccaaaggg ttgttcggca ctatcgtcga caacttctat ctcgtcgcct
      601 tgatcttcgc gatgggtacc agtctgggcc ttgccacgcc gctggtgacc gagtgtatgc
      661 aatggttgtt tggcattccg cataccctgc aactggacgc tatcatcatt acctgctgga
      721 ttatcctcaa cgccatttgc gtcgcttgcg gtctgcaaaa aggggtacgt atcgccagtg
      781 acgtgcgtag ttacctgagc ttcctgatgc tgggttgggt gttcattgtc agcggtgcca
      841 gcttcatcat gaactacttc accgattcgg tggggatgtt gctgatgtat ctgccgcgca
      901 tgttgttcta taccgatccc atcgctaaag gcggcttccc gcagggctgg accgtgttct
      961 actgggcatg gtgggtgatt tatgctatcc agatgagtat cttcctcgcc cgcatctccc
     1021 gtggtcgtac tgtgcgtgaa ctgtgcttcg gcatggtgct ggggctgaca gcgtcaacct
     1081 ggatcctgtg gactgtactc ggtagtaaca ctctgctgtt gatagataaa aacatcatca
     1141 acattccaaa tctgatcgaa cagtacggtg tggcgcgcgc catcattgaa acctgggccg
     1201 ctctgccact cagcaccgcc accatgtggg gcttcttcat cctctgcttt attgccaccg
     1261 ttacgctggt taacgcctgc tcttataccc tggcgatgtc cacttgccgc gaagtacgcg
     1321 atggtgaaga accacctctg ctggtgcgta tcggttggtc aattctggtt ggcattatcg
     1381 gtattgttct gctggcgctc ggcggcctga aaccgattca aaccgccatt atcgccggag
     1441 gatgcccgct gttcttcgtc aacattatgg tgacgctctc ctttattaaa gacgcgaaac
     1501 agaactggaa agattaatta acccccaaaa tatcaagagg ttgaaagatg gattttaatt
     1561 taaatgatga gcaggaactg tttgtcgccg gtatccgcga actgatggcc agcgaaaact
     1621 gggaggccta ttttgccgag tgcgaccgtg acagcgtcta cccggaacgt tttgtcaaag
     1681 cactggcgga tatgggtatc gacagtctgc tgatccctga agagcacggt ggtctggacg
     1741 cggggtttgt tactctcgcc gccgtgtgga tggagctggg acgtctgggg gcaccaacct
     1801 atgtgctgta ccagttgccg ggcgggttca acaccttcct gcgcgaaggc acacaagagc
     1861 agatcgacaa gattatggct ttccgcggca ccggtaagca gatgtggaac tcagcgatta
     1921 ccgaaccggg cgcgggctcc gacgtgggta gcctgaaaac gacttatacc cgtagaaatg
     1981 gtaagattta tcttaatggt agtaagtgtt ttattaccag cagcgcctac accccgtaca
     2041 tcgtggtgat ggcgcgcgac ggggcttctc cggacaaacc tgtctacacc gaatggtttg
     2101 ttgatatgag caaaccgggc atcaaagtga ccaaacttga aaagctcggt ctgcgtatgg
     2161 atagctgctg tgaaatcacc tttgacgacg tggaactgga cgagaaagac atgttcggtc
     2221 gggaaggtaa cggctttaac cgcgtcaaag aagagttcga ccatgaacgt ttcctggtag
     2281 ccctcaccaa ctacggtacg gcgatgtgcg cctttgaaga tgcggcgcgc tacgccaatc
     2341 agcgcgtgca gtttggcgag gctattggtc gtttccagtt gattcaggaa aaattcgccc
     2401 acatggcgat caaattaaac tccatgaaaa acatgctgta tgaagcagcg tggaaagcag
     2461 acaacggcac catcacctct ggcgatgcag cgatgtgcaa atacttctgc gccaatgcgg
     2521 catttgaagt tgtggatagc gcaatgcagg tgctgggcgg tgtcgggatt gcgggcaacc
     2581 accgcatcag ccgcttctgg cgtgatctgc gtgtagaccg cgtctccggg ggatctgacg
     2641 aaatgcagat cctgacgctg ggtcgcgcgg tgctgaagca ataccgctaa gtccgtgcgc
     2701 tgcctgatgc gacgctaatg cgtcttatca ggcctacggg agcacgaatg taggccggat
     2761 aaagcgttta cgccgcatcc ggcagtcatg cgctacataa atttttcagg agctaataat
     2821 ggatcatcta cccatgccga aattcgggcc gttggccgga ttgcgcgttg tcttctccgg
     2881 tatcgaaatc gccgggccgt tcgccgggca aatgttcgca gaatggggtg cggaagttat
     2941 ctggatcgag aacgtcgcct gggccgacac cattcgcgtt caaccgaact acccgcagct
     3001 ctcccgccgc aatttgcacg cgctgtcgtt aaatattttc aaagatgaag gccgcgaagc
     3061 gtttctgaaa ttaatggaaa ccaccgatat cttcatcgaa gccagtaaag gtccggcctt
     3121 tgcccgtcgt ggcattaccg atgaagtact gtggcagcac aacccgaaac tggttatcgc
     3181 tcacctgtcc ggttttggtc agtacggcac cgaggagtac accaatcttc cggcctataa
     3241 caccatcgcc caggccttca gtggttacct gattcagaac ggtgatgttg accagccaat
     3301 gcctgccttc ccgtataccg ccgattactt ttctggcctg accgccacca cggcggcgct
     3361 ggcagcactg cataaagtgc gtgaaaccgg taaaggcgaa agtatcgaca tcgccatgta
     3421 tgaagtgatg ctgcgtatgg gccagtactt catgatggat tacttcaacg gcggcgaaat
     3481 gtgcccgcgc atgagcaaag gtaaagatcc ctactacgcc ggttgcggtc tgtataaatg
     3541 cgccgacggc tacatcgtga tggaactggt gggcattacc caaattgaag agtgctttaa
     3601 agatattggc ctcgcacatc tgctcggtac gccggaaatc ccggaaggca ctcagcttat
     3661 ccaccgtatc gaatgccctt acggcccact ggtagaagag aaactcgatg cctggctggc
     3721 ggcacatacc atcgcagaag ttaaagaacg ctttgccgaa ctgaatatcg cctgtgccaa
     3781 agtactgacc gtaccggaac tggaaagcaa tccacagtat gttgcccgtg aatcaatcac
     3841 tcagtggcaa acgatggatg gtcgcacctg caaagggccg aacatcatgc cgaaattcaa
     3901 aaataacccc ggacaaatct ggcgcggaat gccctcacat ggcatggaca cggctgccat
     3961 tttgaaaaat atcggctaca gcgaaaacga cattcaggag ttggtcagca aaggtctggc
     4021 caaagttgag gactaaacgc gtggcgtcag tcggcttgcc tgactagcgc ccgcccaata
     4081 acaaacgaac aaaaatggat agaggtgcaa tggatatcat tggcggacaa catctacgtc
     4141 aaatgtggga cgatctggcg gacgtttacg gtcataaaac ggcgctgatt tgtgaatcca
     4201 gcggcggagt cgttaaccgg tatagttatc ttgagttaaa tcaggagatt aaccgcacgg
     4261 caaacctgtt ttatacgctg gggattcgca aaggcgacaa ggttgcacta catctcgaca
     4321 actgcccgga atttatcttt tgctggttcg ggctggcaaa aattggcgcg attatggtgc
     4381 cgattaacgc ccgcctgtta cgcgaggaaa gcgcgtggat cctgcaaaat agccaggcgt
     4441 gcctgctggt gaccagtgcg caattctatc ctatgtatca acagattcag caggaagatg
     4501 ccactcaatt gcggcacatt tgcctgacag atgtggcact tcccgctgat gatggcgtga
     4561 gttcgtttac tcaactgaaa aatcaacaac ctgccacctt gtgctatgca ccgccgctat
     4621 tgactgacga tacggcggaa attctcttca cctccggcac cacctcccga ccgaaaggtg
     4681 tggtgattac ccattacaac ctgcgcttcg ctggatatta ctccgcctgg cagtgtgcac
     4741 tgcgtgacga tgacgtctac ctgacggtaa tgcctgcgtt tcatatcgat tgccagtgta
     4801 ctgcggcgat ggcggcgttt tctgccgggg ccacctttgt gctggtcgag aaatacagcg
     4861 cccgcgcctt ctggggacag gtgcagaagt accgcgccac cattaccgaa tgtattccga
     4921 tgatgatccg tacgttgatg gtgcagccgc cttcagcgaa cgatcggcaa caccgcctgc
     4981 gggaagtgat gttttatctc aacttgtcgg agcaggaaaa agatgcgttt tgtgaacgct
     5041 tcggcgttcg cttgctgacg tcttatggga tgacggaaac cattgtgggc attatcggcg
     5101 atcgccctgg cgataaacga cgctggccgt cgattggtcg ggcggggttt tgctacgaag
     5161 cggagatccg cgacgatcac aatcgcccgc tcccggcagg tgagatcggt gaaatctgta
     5221 ttaaaggcgt accagggaaa accatcttca aagagtattt tctcaacccg aaagccactg
     5281 cgaaagtgct ggaagccgac ggctggctac ataccggcga taccggatac tgcgacgaag
     5341 agggcttttt ttatttcgtc gatcgccgct gcaatatgat caaacgcggt ggcgagaatg
     5401 tctcctgcgt ggagctggaa aatatcatcg ccacccatcc aaaaattcag gatatcgtgg
     5461 ttgtgggtat taaagattcg attcgcgatg aagccatcaa agcatttgtg gtgctgaatg
     5521 aaggtgaaac attgagcgaa gaggaatttt tccgcttctg cgaacaaaat atggcgaaat
     5581 ttaaagtgcc ctcttatctg gagatcagaa aagatctgcc acgtaattgc tcggggaaaa
     5641 taattagaaa gaatctgaaa taaatgaaac agcagggaac taccctgcca gctaataatc
     5701 acgctataaa acaatatgca ttttttgcag ggatgttgtc gtccctgaaa aagcaaaaat
     5761 ggagaaaagg aatgagtgaa tcattacatc tgacccgcaa tggatcaatt ctggaaatta
     5821 cccttgatcg tccaaaagcg aatgctattg atgcaaaaac cagctttgaa atgggcgaag
     5881 tatttctaaa tttccgtgac gatccgcaat tacgtgtcgc cattattacc ggtgccggag
     5941 agaagttctt ttccgcgggc tgggatttaa aagcggcagc agaaggcgaa gcaccggatg
     6001 ctgactttgg cccgggtgga tttgcgggat taaccgaaat tttcaatctc gacaaaccgg
     6061 ttatcgcagc tgtgaacggc tatgcctttg gcggcggttt cgaactggcg ctggcggcag
     6121 attttattgt ttgtgccgat aacgccagct tcgccctgcc ggaagccaag ctgggcatcg
     6181 ttcctgacag cggcggcgtg ctgcgtctac cgaagatcct gccgcctgcc atcgtcaatg
     6241 aaatggtgat gaccggcaga cgaatgggcg cagaagaggc gctgcgttgg gggatagtca
     6301 accgcgtggt tagccaggcg gaactgatgg ataacgcccg cgaactggcg cagcagctgg
     6361 ttaacagcgc cccgctggcg attgcggcgc tgaaagagat cttccgcacc accagcgaaa
     6421 tgccggtaga agaagcgtat cgctatattc gcagcggcgt gttgaaacac tatccgtcgg
     6481 ttctgcattc ggaggatgcc attgaagggc cgctggcgtt tgccgagaag cgcgatccgg
     6541 tgtggaaagg acgttaacaa ccgtgagcta ttacgccttt gaggggttaa ttccggtggt
     6601 tcacccgacg gcgtttgtcc atcccagtgc cgtcttgatt ggcgatgtga ttgtgggagc
     6661 cggtgtctac atcggcccac tcgcctcact gcgtggtgac tacgggcggt tgatcgtgca
     6721 agcgggagcc aatattcagg atggctgcat tatgcatggc tactgcgaca ctgacaccat
     6781 cgttggggaa aacggccata tcgggcacgg agcgatcctg catggttgtg tgattggtcg
     6841 cgatgcattg gtcgggatga acagcgtgat tatggatggc gcggtcattg gcgaagagag
     6901 cattgttgcc gccatgagct ttgtcaaagc aggctttcac ggcgagaaac gccagttgtt
     6961 gatgggtacg ccagcccgcg ctgtacgcag tgttagtgac gacgagttac actggaaacg
     7021 gttgaatacc aaagagtatc aggatcttgt cgggcgctgt catgcatcgt tacatgaaac
     7081 acagccactg aggcaaatgg aggaaaatcg cccccgtttg caggggacga cggatgtgac
     7141 gcctaaacgg tgatcttgcc ggatgcgacc tggttgctgg ataagatgct acaacatcgc
     7201 atctggcaag caggacgccc ggtatcaacg agataaaatt aacgacgcat actctttgac
     7261 aacatcatct gccacttctg ctcgcggtta agttccgtga gcggaacggc aggaatacgc
     7321 ggcgtctttc ttacgccgac taatttatcc cggctgttat tgcgcaggcg cgcgtaaatt
     7381 tgctcatcga tatcgaccac ttttaccagt cgctggcact ggcatccccg tccttccagc
     7441 ttattaggga tcatcttaac ttcgcagctt atttctgcga cttccgacag aatataagtc
     7501 agggtattaa ccgccttgct gtgttcaata tcgaaatgaa tagagatctc tcttgctatc
     7561 acccaccgat tttcagccat catccattcg gcgattaaca agtagagtgg tttttcaaca
     7621 tatccttcac acatacgaac tccagtttca caaattaaaa gtctggctat gactcgtttt
     7681 gaagcaatta aacaaggcca tattaaaatt gtggatatat cgatagtatg caacttcacc
     7741 gtcgataagt gtgagctaaa tcctgcttat gtcatcaaaa atatcgattc cccaaaagat
     7801 ttacttaatg ggcaaaaaaa acggtcctca tcagagaacc gtattactta ttcgatcaca
     7861 ttagcggatg aaaaatatcc gccatgacac gctattattt gatctgcgcg tgcatttcct
     7921 gcaccgaaat tactttttcg gtcgcgtcgg cattcagcgc catcgcggta gcgaaaccac
     7981 cgttcagggt ggtgtcataa tgcactttat attgcagcgc actgcgacgg atcacgcggg
     8041 agtcttcaat cgcgcgacgg cctgaagtgg tgttgatgat gtaggtatat tcgccattct
     8101 tgatgcggtc ctgaatgtgc ggacggcctt catgcacctt gttcaccaga cgcggattaa
     8161 tgcccgcttc gcccagcaca atcgccgtgc cgtgggtcgc atccagctcg aagccctgtt
     8221 tcagcagttt tgccgccagg tccaccacgc gttctttatc gccttcgcgc acggaaagca
     8281 acgcacgacc gtgtttcttc atggtggagt tgctgcccag ctgcgctttg gcaaacgctt
     8341 cagcgaaggt gcggcccacg cccatcactt ccccggtgga gcgcatttct ggccctaaca
     8401 gcgggtcaac acccgggaat ttattgaacg gcagcaccac ttctttcacc gagtagtacg
     8461 gcgggataac ttctttggtt acgccctgct cagccagcga tttgccagcc atcacacgcg
     8521 ccgccacttt tgccagcggt acgccggtgg ctttggagac gaacggaacg gtacgcgccg
     8581 cacgcgggtt aacttcaatc aggtagactt cgttgttttt caccgcaaac tgcacgttca
     8641 tcagaccgcg cacctgcaat tcgaaggcca gtttctgcac ctgctggcgc atcacatcct
     8701 gaatttcctg acttaaggtg taggctggca gagaacatgc ggagtcaccg gagtgcacgc
     8761 ccgcctgctc gatgtgctcc atgatgccgc caatcagcac catttcgccg tcgcagatgg
     8821 catccacgtc aacttctacc gcgtcatcaa ggaagtgatc cagcaacact ggcgcatcgt
     8881 tagacacgct gaccgccgtc tggaagtagc gacgcaggtc agcttcgtca tagacgattt
     8941 ccatcgcccg accgccgaga acgtaagacg gacgtaccac cagcgggtag ccaatctctt
     9001 tcgccttctc taccgccatt tcaatagcgg taacggtggc gttcgccggt tgtttcagtt
     9061 tcagacgctc aaccgcatgc tggaagcgtt cacggtcttc tgcacggtcg atagcatccg
     9121 ggctggtgcc gataaccggt acgccagcag cttccagcgc gcgcgccagt ttcagcgggg
     9181 tctgaccgcc gtactgaacg ataacgcctt tcggcttctc aatacgcacg atttctagca
     9241 catcttccag tgttaccggc tcgaagtaga ggcgatcgga agtgtcgtag tcggtggaga
     9301 cggtttccgg gttacagtta accataatgg tttcgtaacc gtcttcgcgc agtgccagcg
     9361 aggcgtgtac acagcagtag tcgaattcga taccctgacc gatacggttc gggccgccgc
     9421 cgagaaccat gattttttca cggtcggtag acggattcgc ttcgcactct tcttcataag
     9481 tggagtacat gtaagcggtg tcggtggcga actctgccgc acaggtatcc acgcgcttat
     9541 aaaccgggtg caggtcatac tggtcgcgca gcttgcggat ttccgcttcg cgcacgcccg
     9601 ccagttttgc cagacgcgca tcggcaaagc ctttgcgttt cagctggcgc aggaattcag
     9661 cgttcaggcc agtgatgccc acttccgcta ctttctcttc cagacgcacc agttcttcaa
     9721 tctgtaccag gaaccagcgg tcaatattgg tcaggttgaa cacgccgtcc acagacaggc
     9781 ccgcacggaa ggcatcggcg atgtaccaga tacgctctgc gccagcatct ttcagttcgc
     9841 gacggatttt ggttaacgct tccgggtcat cgaggctcac tttcgggtcg aatccagtcg
     9901 caccgacttc caggccgcgc agcgcttttt gcagggattc ctgctgcgtg cgaccaatcg
     9961 ccatcacttc gccaaccgat ttcatctgag tggtcagacg gtcgttagca ccagcgaatt
    10021 tttcgaagtt gaagcgagga attttagtaa ccacatagtc gatggacggc tcgaaggagg
    10081 ccggagtacg tccgccagtg atgtcgttca tcagttcgtc gagggtgtaa cccaccgcca
    10141 gtttcgccgc cactttagca atcgggaaac cggtcgcttt cgacgccagc gccgaagaac
    10201 gggacacgcg cgggttcatt tcgataacaa tcaggcgacc gtttttcggg ttcaccgcaa
    10261 actggacatt ggaaccaccg gtttcaacgc cgatttcacg cagcaccgcc atcgaggcgt
    10321 tacgcatgat ttgatattct ttgtcggtca gcgtttgggc tggcgcgaca gtgatggagt
    10381 caccggtatg gatgcccatc gcatcaaagt tttcgataga gcagacgatg atgcagttgt
    10441 cgtttttatc acgcaccact tccatctcgt actctttcca gccaatcagc gactcatcaa
    10501 tcagcaactc tttggtcgga gagagatcca gaccgcgggc gcaaatttct tcaaactctt
    10561 cgcggttata agcgataccg ccgccgctac cgcccatggt aaaggatggg cgaataatgc
    10621 acgggaagcc cacgtcagcg gcaaccgcca gcgcttcttc catcgtgtgt gcgataccgg
    10681 aacgcgcggt ttccagacca attttcttca tcgctacgtc gaaacgacgg cggtcttctg
    10741 ctttatcaat cgcatcggcg gtcgcaccaa tcatagtcac gccgaactct tccaacacgc
    10801 cctgacgctc cagctccagc gcgcagttca gcgccgtctg accgcccatc gttggcagca
    10861 ccgcgtccgg gcgctctttt tcaataatct tgcgtaccac ttcccagtga atcggctcga
    10921 tgtaggtcgc atcggccatt tccgggtcgg tcatgatggt cgccgggttg gagttcacca
    10981 gaatgacccg gtaaccctct tcgcgcaggg ctttacatgc ttgcgcgcca gagtagtcaa
    11041 actcacacgc ctgaccgata acaatcgggc ccgcacccag aatcaggata ctttttatat
    11101 ctgtacgttt tggcatggct cttttactcc tgattactta gcggttttac ggtactgctc
    11161 aattaactcg ataaagtggt cgaacaacgg cgcggcgtcg tgtggaccag ggctggcttc
    11221 agggtgcccc tggaagctga acgccggttt atcggtgcga tgaatgccct gtaacgtacc
    11281 gtcgaacagg gatttatgcg tgacacgcag gtttgctggt aatgttgctt cgtccaccgc
    11341 aaaaccgtgg ttctgggcgg tgatcatcac cacgtttttc tcaacatctt taaccggatg
    11401 gttgccgccg tggtgaccaa atttcatttt gacagtcttc gcaccgctcg ccagcgccag
    11461 cagctgatga ccgaggcaga tgccgaagac cggaatatcg gtttcgagga atttctggat
    11521 ggcggtaatg gcgtaatcgc acggggccgg gtcgccagga ccgttggaga ggaagatacc
    11581 gtctggattc attttcagca catcttccgc agaagtttgc gccggaacga tggtcaggcg
    11641 acagcctctg tccaccaaca tgcgcaggat gttgcgcttg gcaccaaaat cataagccac
    11701 gacgtggaac ggcagctcgt cttctttttt cgcttctggc aggccgccgg tcaacgtcca
    11761 gctcccttgt gtccagctat aggcttctgc ggtggtcact tcttttgcca gatccatgcc
    11821 attcagacct gggaacgcgc gggctttttc taacgccaac gccgcatccg ggttatcgcc
    11881 tgcgataatg cagccattct gtgcgccttt ctcgcgcagt aaacgcgtca gcttacgggt
    11941 atcgatatcg gcaatcgcca cgatgttatg gcgcttcagg taagaagaga ggtcttcggt
    12001 attacggaag ttgctggcaa tcagcggcag gtcgcgaatc accagacctt gtgcatgtac
    12061 ctgagaagat tcttcatcgg cgtcattagt gccgacattg ccaatatggg gataagtaag
    12121 agtaacgatt tgacgagaat aggaaggatc agtgaggatt tcttgataac cggtcattga
    12181 agtattgaaa acgacttccc caaccgccga acctgttgcc cctatggccc gaccgtgaaa
    12241 ctgggttccg tcttccagaa ccaatagcgc tgacttaatc aaaacaccct ccagagaata
    12301 ttcactcact ttat
//
LOCUS       DAZKHF020000131        11896 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2201S31241
            SAMN43267800-rid25106413.denovo.132, whole genome shotgun sequence.
ACCESSION   DAZKHF020000131 DAZKHF020000000
VERSION     DAZKHF020000131.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267800
            Sequence Read Archive: SRR30307263
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 11896)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11896)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 11896)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350581.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350581.2
            Genome Coverage       :: 151x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:21:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,652
            CDSs (total)                      :: 5,557
            Genes (coding)                    :: 5,320
            CDSs (with protein)               :: 5,320
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 237
            CDSs (without protein)            :: 237
            Pseudo Genes (ambiguous residues) :: 0 of 237
            Pseudo Genes (frameshifted)       :: 102 of 237
            Pseudo Genes (incomplete)         :: 130 of 237
            Pseudo Genes (internal stop)      :: 52 of 237
            Pseudo Genes (multiple problems)  :: 41 of 237
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11896
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267800-rid25106413.denovo.132"
                     /serotype="O 26:H11"
                     /isolate="2201S31241"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-01"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            complement(208..855)
                     /gene="ychE"
                     /locus_tag="ACHOSC_RS14725"
                     /old_locus_tag="pgaptmp_002945"
     CDS             complement(208..855)
                     /gene="ychE"
                     /locus_tag="ACHOSC_RS14725"
                     /old_locus_tag="pgaptmp_002945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAAT family transporter YchE"
                     /protein_id="HGU4548848.1"
                     /translation="MIQTFFDFPVYFKFFIGLFALVNPVGIIPVFISMTSYQTAAARN
                     KTNLTANLSVAIILWISLFLGDTILQLFGISIDSFRIAGGILVVTIAMSMISGKLGED
                     KQNKQEKSETAVRESIGVVPLALPLMAGPGAISSTIVWGTRYHSISYLFGFFVAIALF
                     ALCCWGLFRMAPWLVRVLRQTGINVITRIMGLLLMALGIEFIVTGIKGIFPGLLN"
     gene            complement(886..939)
                     /gene="ychE"
                     /locus_tag="ACHOSC_RS14730"
                     /old_locus_tag="pgaptmp_002946"
     CDS             complement(886..939)
                     /gene="ychE"
                     /locus_tag="ACHOSC_RS14730"
                     /old_locus_tag="pgaptmp_002946"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YchT"
                     /protein_id="HGU4548849.1"
                     /translation="MLSKGVLSARLFNLIYG"
     gene            1332..4007
                     /gene="adhE"
                     /locus_tag="ACHOSC_RS14735"
                     /old_locus_tag="pgaptmp_002947"
                     /gene_synonym="adhC"
     CDS             1332..4007
                     /gene="adhE"
                     /locus_tag="ACHOSC_RS14735"
                     /old_locus_tag="pgaptmp_002947"
                     /gene_synonym="adhC"
                     /EC_number="1.1.1.1"
                     /EC_number="1.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004022 - alcohol dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006066 - alcohol metabolic process
                     [Evidence IEA];
                     GO_process: GO:0015976 - carbon utilization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional acetaldehyde-CoA/alcohol
                     dehydrogenase"
                     /protein_id="HGU4548850.1"
                     /translation="MAVTNVAELNALVERVKKAQREYASFTQEQVDKIFRAAALAAAD
                     ARIPLAKMAVAESGMGIVEDKVIKNHFASEYIYNAYKDEKTCGVLSEDDTFGTITIAE
                     PIGIICGIVPTTNPTSTAIFKSLISLKTRNAIIFSPHPRAKDATNKAADIVLQAAIAA
                     GAPKDLIGWIDQPSVELSNALMHHPDINLILATGGPGMVKAAYSSGKPAIGVGAGNTP
                     VVIDETADIKRAVASVLMSKTFDNGVICASEQSVVVVDSVYDAVRERFATHGGYLLQG
                     KELKAVQDVILKNGALNAAIVGQPAYKIAELAGFSVPENTKILIGEVTVVDESEPFAH
                     EKLSPTLAMYRAKDFEDAVEKAEKLVAMGGIGHTSCLYTDQDNQPARVSYFGQKMKTA
                     RILINTPASQGGIGDLYNFKLAPSLTLGCGSWGGNSISENVGPKHLINKKTVAKRAEN
                     MLWHKLPKSIYFRRGSLPIALDEVITDGHKRALIVTDRFLFNNGYADQITSVLKAAGV
                     ETEVFFEVEADPTLSIVRKGAELANSFKPDVIIALGGGSPMDAAKIMWVMYEHPETHF
                     EELALRFMDIRKRIYKFPKMGVKAKMIAVTTTSGTGSEVTPFAVVTDDATGQKYPLAD
                     YALTPDMAIVDANLVMDMPKSLCAFGGLDAVTHAMEAYVSVLASEFSDGQALQALKLL
                     KEYLPASYHEGSKNPVARERVHSAATIAGIAFANAFLGVCHSMAHKLGSQFHIPHGLA
                     NALLICNVIRYNANDNPTKQTAFSQYDRPQARRRYAEIADHLGLSAPGDRTAAKIEKL
                     LAWLETLKAELGIPKSIREAGVQEADFLANVDKLSEDAFDDQCTGANPRYPLISELKQ
                     ILLDTYYGRDYVEGETAAKKEAAPAKAEKKAKKSA"
     gene            4131..5027
                     /locus_tag="ACHOSC_RS14740"
                     /old_locus_tag="pgaptmp_002948"
                     /pseudo
     CDS             4131..5027
                     /locus_tag="ACHOSC_RS14740"
                     /old_locus_tag="pgaptmp_002948"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012904641.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS4 family transposase"
     gene            complement(5309..5926)
                     /gene="tdk"
                     /locus_tag="ACHOSC_RS14745"
                     /old_locus_tag="pgaptmp_002949"
     CDS             complement(5309..5926)
                     /gene="tdk"
                     /locus_tag="ACHOSC_RS14745"
                     /old_locus_tag="pgaptmp_002949"
                     /EC_number="2.7.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidine kinase"
                     /protein_id="HGU4548851.1"
                     /translation="MAQLYFYYSAMNAGKSTALLQSSYNYQERGMRTVVYTAEIDDRF
                     GAGKVSSRIGLSSPAKLFNQNSSLFDEIRAEHEQQAIHCVLVDECQFLTRQQVYELSE
                     VVDQLDIPVLCYGLRTDFRGELFIGSQYLLAWSDKLVELKTICFCGRKASMVLRLDQA
                     GRPYNEGEQVVIGGNERYVSVCRKHYKEALQVGSLTAIQERHRHD"
     gene            6531..6944
                     /gene="hns"
                     /locus_tag="ACHOSC_RS14750"
                     /old_locus_tag="pgaptmp_002950"
     CDS             6531..6944
                     /gene="hns"
                     /locus_tag="ACHOSC_RS14750"
                     /old_locus_tag="pgaptmp_002950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0071824 - protein-DNA complex organization
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histone-like nucleoid-structuring protein H-NS"
                     /protein_id="HGU4548852.1"
                     /translation="MSEALKILNNIRTLRAQARECTLETLEEMLEKLEVVVNERREEE
                     SAAAAEVEERTRKLQQYREMLIADGIDPNELLNSLASVKSGTKAKRAQRPAKYSYVDE
                     NGETKTWTGQGRTPAVIKKAMDEQGKSLDDFLIKQ"
     gene            complement(7088..7996)
                     /gene="galU"
                     /locus_tag="ACHOSC_RS14755"
                     /old_locus_tag="pgaptmp_002951"
     CDS             complement(7088..7996)
                     /gene="galU"
                     /locus_tag="ACHOSC_RS14755"
                     /old_locus_tag="pgaptmp_002951"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UTP--glucose-1-phosphate uridylyltransferase
                     GalU"
                     /protein_id="HGU4548853.1"
                     /translation="MAAINTKVKKAVIPVAGLGTRMLPATKAIPKEMLPLVDKPLIQY
                     VVNECIAAGITEIVLVTHSSKNSIENHFDTSFELEAMLEKRVKRQLLDEVQSICPPHV
                     TIMQVRQGLAKGLGHAVLCAHPVVGDEPVAVILPDVILDEYESDLSQDNLAEMIRRFD
                     ETGHSQIMVEPVADVTAYGVVDCKGVELAPGESVPMVGVVEKPKADVAPSNLAIVGRY
                     VLSADIWPLLAKTPPGAGDEIQLTDAIDMLIEKETVEAYHMKGKSHDCGNKLGYMQAF
                     VEYGIRHNTLGTEFKAWLEEEMGIKK"
     gene            complement(8198..9211)
                     /gene="rssB"
                     /locus_tag="ACHOSC_RS14760"
                     /old_locus_tag="pgaptmp_002952"
     CDS             complement(8198..9211)
                     /gene="rssB"
                     /locus_tag="ACHOSC_RS14760"
                     /old_locus_tag="pgaptmp_002952"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator RssB"
                     /protein_id="HGU4548854.1"
                     /translation="MTQPLVGKQILIVEDEPVFRSLLDSWFSSLGATTVLAADGVDAL
                     ELLGGFTPDLMICDIAMPRMNGLKLLEHIRNRGDQTPVLVISATENMADIAKALRLGV
                     EDVLLKPVKDLNRLREMVFACLYPSMFNSRVEEEERLFRDWDAMVDNPAAAAKLLQEL
                     QPPVQQVISHCRVNYRQLVAADKPGLVLDIAALSENDLAFYCLDVTRAGHNGVLAALL
                     LRALFNGLLQEQLAHQNQRLPELGALLKQVNHLLRQANLPGQFPLLVGYYHRELKNLI
                     LVSAGLNATLNTGEHQVQISNGVPLGTLGNAYLNQLSQRCDAWQCQIWGTGGRLRLML
                     SAE"
     gene            complement(9303..10208)
                     /gene="rssA"
                     /locus_tag="ACHOSC_RS14765"
                     /old_locus_tag="pgaptmp_002953"
     CDS             complement(9303..10208)
                     /gene="rssA"
                     /locus_tag="ACHOSC_RS14765"
                     /old_locus_tag="pgaptmp_002953"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707140.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004622 - lysophospholipase activity
                     [Evidence IEA];
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase RssA"
                     /protein_id="HGU4548855.1"
                     /translation="MRKIKIGLALGSGAARGWSHIGVINALKKVGIEIDIVAGCSIGS
                     LVGAAYACDRLSALEDWVTSFSYWDVLRLMDLSWQRGGLLRGERVFNQYREIMPETEI
                     ENCSRRFAAVATNLSTGRELWFTEGDLHLAIRASCSIPGLMAPVAHNGYWLVDGAVVN
                     PIPISLTRALGADIVIAVDLQHDAHLMQQDLLSFNVSEENSENGDSLPWHARLKERLG
                     SITTRRAVTAPTATEIMTTSIQVLENRLKRNRMAGDPPDILIQPVCPQISTLDFHRAH
                     AAIAAGQLAVEKKMDELLPLVRTNI"
     gene            10321..10779
                     /gene="ychJ"
                     /locus_tag="ACHOSC_RS14770"
                     /old_locus_tag="pgaptmp_002954"
     CDS             10321..10779
                     /gene="ychJ"
                     /locus_tag="ACHOSC_RS14770"
                     /old_locus_tag="pgaptmp_002954"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YchJ family protein"
                     /protein_id="HGU4548856.1"
                     /translation="MSQLCPCGSAVEYSLCCHPYVSGEKVAPDPEHLMRSRYCAFVMQ
                     DADYLIKTWHPSCGAAALRAELMAGFAHTEWLGLTVFEHCWQDADNIGFVSFVARFTE
                     GGKTGAIIERSRFLKENGQWYYIDGTRPQFGRNDPCPCGSGKKFKKCCGQ"
     gene            10829..11671
                     /gene="purU"
                     /locus_tag="ACHOSC_RS14775"
                     /old_locus_tag="pgaptmp_002955"
     CDS             10829..11671
                     /gene="purU"
                     /locus_tag="ACHOSC_RS14775"
                     /old_locus_tag="pgaptmp_002955"
                     /EC_number="3.5.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008864 - formyltetrahydrofolate
                     deformylase activity [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formyltetrahydrofolate deformylase"
                     /protein_id="HGU4548857.1"
                     /translation="MHSLQRKVLRTICPDQKGLIARITNICYKHELNIVQNNEFVDHR
                     TGRFFMRTELEGIFNDSTLLADLDSALPEGSVRELNPAGRRRIVILVTKEAHCLGDLL
                     MKANYGGLDVEIAAVIGNHDTLRSLVERFDIPFELVSHEGLSRNEHDQKMADAIDAYQ
                     SDYVVLAKYMRVLTPEFVARFPNKIINIHHSFLPAFIGARPYHQAYERGVKIIGATAH
                     YVNDNLDEGPIIMQDVIHVDHTYTAEDMMRAGRDVEKNVLSRALYKVLAQRVFVYGNR
                     TIIL"
BASE COUNT         3217 a         2950 c         2705 g         3024 t
ORIGIN      
        1 caattattct tcagcaagcc caggattatg atgtggcgaa aataacatga tggtgaggag
       61 aagcagttaa ggaaatcctt ataactaact gtaatttgag aagaatattt ttacctattc
      121 cttttgcttt tgataactgc gcgggaacaa agtaaaaata atgacgaagt aaacggagca
      181 tggcagctcc gtttcatttg aaaggaatta attaagcagg ccggggaaaa tccccttaat
      241 accagtaacg ataaattcaa tccccaatgc catcagcaat agccccataa tacgcgtaat
      301 cacgttgatg ccggtctggc gtaaaacccg taccagccac ggtgccatgc ggaacaatcc
      361 ccagcaacat aaagcgaaca atgcaatagc cacaaagaaa ccaaacagat agctaatgct
      421 gtgataacgc gtaccccaga cgatggtaga actgatcgcc cctggccccg ccatcaacgg
      481 caacgccaat ggcaccacac caatgctttc acgtaccgcg gtttctgatt tttcttgctt
      541 gttctgttta tcctcgccaa gcttgccgct gatcatcgac atcgctatcg tcaccaccag
      601 gatgcccccg gcgatacgga acgaatcaat tgatatacca aaaagttgca gaatcgtgtc
      661 gccgagaaaa agcgagatcc acaagataat ggccacagac aggttggctg taaggttagt
      721 tttgtttcgc gctgccgctg tctgataact ggtcatgctg ataaagacgg gaataatccc
      781 taccgggttg accagcgcaa ataacccgat gaaaaatttg aagtaaacgg gaaaatcaaa
      841 aaaggtctga atcacggtta gctccgaagc aaaagccgga taatgttagc cataaataag
      901 gttgaaaaga cgcgctgaca atacgccttt tgacagcatt tttcacctcc taactactta
      961 aaattgctat cattcgttat tgttatctag ttgtgcaaaa catgctaatg tagccaccaa
     1021 atcatactac aatttattaa ctgttagcta taatggcgaa aagcgatgct gaaaggtgtc
     1081 agctttgcaa aaatttgatt tggatcacgt aatcggtacc cagaagtgag taatcttgct
     1141 tacgccacct ggaagtgacg cattagagat aataactcta atgtttaaac tcttttagta
     1201 aatcacagtg agtgtgagcg cgagtaagct tttgattttc ataggttaag caaatcatca
     1261 ccgcactgac tatactctcg tattcgagca gatgatttac taaaaaagtt taacattatc
     1321 aggagagcat tatggctgtt actaatgtcg ctgaacttaa cgcactcgta gagcgtgtaa
     1381 aaaaagccca gcgtgaatat gccagtttca ctcaagagca agtagacaaa atcttccgcg
     1441 ccgccgctct ggctgctgca gatgctcgaa tcccactcgc gaaaatggcc gttgccgaat
     1501 ccggcatggg tatcgtcgaa gataaagtga tcaaaaacca ctttgcttct gaatatatct
     1561 acaacgccta taaagatgaa aaaacctgtg gtgttctgtc tgaagacgac acttttggta
     1621 ccatcactat cgctgaacca atcggtatta tttgcggtat cgttccgacc actaacccga
     1681 cttcaactgc tatcttcaaa tcgctgatca gtctgaagac ccgtaacgcc attatcttct
     1741 ccccgcaccc gcgtgcaaaa gatgccacca acaaagcggc tgatatcgtt ctgcaggctg
     1801 ctatcgctgc cggtgctccg aaagatctga tcggctggat cgatcaacct tctgttgaac
     1861 tgtctaacgc actgatgcac cacccagaca tcaacctgat cctcgcgact ggtggtccgg
     1921 gcatggttaa agccgcatac agctccggta aaccagctat cggtgtaggc gcgggcaaca
     1981 ctccagttgt tatcgatgaa actgctgata tcaaacgtgc agttgcatct gtactgatgt
     2041 ccaaaacctt cgacaacggc gtaatctgtg cttctgaaca gtctgttgtt gttgttgact
     2101 ctgtttatga cgctgtacgt gaacgttttg caacccacgg cggctatctg ttgcagggta
     2161 aagagctgaa agctgttcag gatgttatcc tgaaaaacgg tgcgctgaac gcggctatcg
     2221 ttggtcagcc agcctataaa attgctgaac tggcaggctt ctctgtacca gaaaacacca
     2281 agattctgat cggtgaagtg accgttgttg atgaaagcga accgttcgca catgaaaaac
     2341 tgtccccgac tctggcaatg taccgcgcta aagatttcga agacgcggta gaaaaagcag
     2401 agaaactggt tgctatgggc ggtatcggtc atacctcttg cctgtacact gaccaggata
     2461 accaaccggc tcgcgtttct tacttcggtc agaaaatgaa aacggctcgt atcctgatta
     2521 acaccccagc gtctcagggt ggtatcggtg acctgtataa cttcaaactc gcaccttccc
     2581 tgactctggg ttgtggttct tggggtggta actccatctc tgaaaacgtt ggtccgaaac
     2641 acctgatcaa caagaaaacc gttgctaagc gagctgaaaa catgttgtgg cacaaacttc
     2701 cgaaatctat ctacttccgc cgtggctccc tgccaatcgc gctggatgaa gtgattactg
     2761 atggccacaa acgtgcgctc atcgtgactg accgcttcct gttcaacaat ggttatgctg
     2821 atcagatcac ttccgtactg aaagcagcag gcgttgaaac tgaagtcttc ttcgaagtag
     2881 aagcggaccc gaccctgagc atcgttcgta aaggtgcaga actggcaaac tccttcaaac
     2941 cagacgtgat tatcgcgctg ggtggtggtt ccccgatgga cgccgcgaag atcatgtggg
     3001 ttatgtacga acatccggaa actcacttcg aagagctggc gctgcgcttt atggatatcc
     3061 gtaaacgtat ctacaagttc ccgaaaatgg gcgtgaaagc gaaaatgatc gctgtcacca
     3121 ccacttctgg tacaggttct gaagtcactc cgtttgcggt tgtaactgac gacgctactg
     3181 gtcagaaata tccgctggca gactatgcgc tgactccgga tatggcgatt gtcgacgcca
     3241 acctggttat ggacatgccg aagtccctgt gtgctttcgg tggtctggac gcagtaactc
     3301 acgccatgga agcttatgtt tctgtactgg catctgagtt ctctgatggt caggctctgc
     3361 aggcactgaa actgctgaaa gaatatctgc cagcgtccta ccacgaaggg tctaaaaatc
     3421 cggtagcgcg tgaacgtgtt cacagtgcag cgactatcgc gggtatcgcg tttgcgaacg
     3481 ccttcctggg tgtatgtcac tcaatggcgc acaaactggg ttcccagttc catattccgc
     3541 acggtctggc aaacgccctg ctgatttgta acgttattcg ctacaatgcg aacgacaacc
     3601 cgaccaagca gactgcattc agccagtatg accgtccgca ggctcgccgt cgttatgctg
     3661 aaattgccga ccacttgggt ctgagcgcac cgggcgaccg tactgctgct aagatcgaga
     3721 aactgctggc atggctggaa acgctgaagg ctgaactggg tattccgaaa tctatccgtg
     3781 aagctggcgt tcaggaagca gacttcctgg cgaacgtgga taaactgtct gaagatgcat
     3841 tcgatgacca gtgcaccggc gctaacccgc gttacccgct gatctccgag ctgaaacaga
     3901 ttctgctgga tacctactac ggtcgtgatt atgtagaagg tgaaactgca gcgaagaaag
     3961 aagctgctcc ggctaaagct gagaaaaaag cgaaaaaatc cgcttaatca gtagcgctgt
     4021 ctggcaacat aaacggcccc ttctgggcaa tgccgatcag ttaaggatta gttgaccgat
     4081 ccttaaactg aggcactata acggcttcca caacagggag ccgttttctt atgccacttc
     4141 ttaatgatct gctcgatttc agtgaccatc cgcttatgcc tccgccctct gcacaactat
     4201 ttgcagaaca ccttcccacc gagtggatac aacactgcct gacgctttct gctcatgcga
     4261 ccgttcgccg ccgtcgttta ccgggggaca tggttatctg gatggtggtg caatgagcca
     4321 attaccgatg ttgttcgccg tctgaacctg agcgcggatg gcgaagcggg gatgaacctg
     4381 ctggcccgca gcgctgtcac ccaggcgcgt cagcgcgtgg gggccgcccc agtggaatgg
     4441 ctcttccgcc agaccgcaca gacaggggcg cggaacgtta cctgaaggat gactggcacg
     4501 gcctgcaact ttttgccatt gatggcgcac agttcaggac acctgataaa cccgagctgc
     4561 gtgaatatta tgggtctgcc aacacatcca ctaagcggca gaacgcctac ccggttatgc
     4621 gtctggtggc cttaatgaac ctgggaagtc acattctgct gaatgccgtg accgcacctt
     4681 accggcagag cgaaactgtg ctggcccact ccatgctcgc caccatcccg gataactcta
     4741 ttacgttgtt tgataaactt ttttacagcg aagacctgct gctgacgctg aaccagaagg
     4801 gttgtaaccg ccactggcta ctgcctgcgt ggaagaatat cgcatcagaa atgatagagc
     4861 tgggaaacac ggcgtcaaca gggaccatcc cgaaacgact ggagcatctt cggggtgctc
     4921 tggaagtggt gttcataaca aaacgccccc gaccatcaag gccgaggtcg gttaagatat
     4981 caaaaacccg ttatcctgtg aaacatagtg ccgctcctct taagtgaacg gcattgccct
     5041 tctggggccg tattttttgt ttacccaaag caacttttcc ataaaccgac agcattagcc
     5101 ttcatcatat ttgcgacgat gtataacgcc taaacacagg gatattgtac tttacaggtc
     5161 acaagtcaac gtcggtgctt aagagccctg tgaggcgtat agcggcgtta aaaaactgcc
     5221 gagaagggta tatagcccgg aagaagtgcg taaaacgaac tgacaggata aaagtgccct
     5281 gctcaccctg tcagtaaaga aattcttatt aatcgtggcg atgcctttcc tgaatagccg
     5341 ttaatgagcc gacttgtaac gcctctttat agtgtttacg gcatacagaa acgtatcgtt
     5401 cattaccacc aattaccacc tgctcacctt cgttataagg tctgcctgct tgatcaagac
     5461 gcagcaccat gcttgcttta cggccacaaa aacagatggt ttttaattca accagtttgt
     5521 cggaccatgc cagtaagtat tggctgccaa taaataattc acctcgaaaa tcggtacgta
     5581 aaccataaca aagtacgggt atatcgagtt gatcgacaac ctccgataat tcatatactt
     5641 gttgtctggt taaaaactgg cattcatcaa ccagtacgca atgaattgcc tgctgttcat
     5701 gttccgcacg aatctcatca aataatgatg aattttggtt aaataatttt gcaggcgatg
     5761 acaaacctat acgcgaactg actttcccgg caccaaagcg atcatcaatt tctgccgtat
     5821 atacgacagt gcgcatgccg cgttcctggt aattgtatga agattgcaac aatgctgtag
     5881 acttacccgc attcattgcg gaatagtaga aatatagctg tgccatcagc cacaggccct
     5941 caatgatatg aaataaagtt ggctggagtt tatcataatt cgtcagtttt tcagctcaac
     6001 agtatgcaca gaagtgggta tatgcgttct cccttacgaa gccttgcata atccttctga
     6061 gctatcatta caactgcctc gcttgttata agcgggtaaa tgactgctgg taactattca
     6121 caatctttaa cctgttgcgc aagtaatagc cctctgttga cctccaggag atagtgcaat
     6181 actaagtcca tgctcttatt gcgactgttc tacttttcat cattcgctta atagggaatt
     6241 ctcgtaaaca caactaatac agaagactga aaggtcgtca gcctacgata atctccccat
     6301 aaaatgtgac atgaatcagg aagttttaac ctcacgtgct gcgaaatcat cggtgtaaat
     6361 agggctatat gccgcgtctt ttctggctaa ttttatgaaa agatatttat tggcggcaca
     6421 aaataaagaa caattttgaa ttccttacat tcctggctat tgcacaactg aatttaaggc
     6481 tctattatta cctcaacaaa ccaccccaat ataagtttga gattactaca atgagcgaag
     6541 cacttaaaat tctgaacaac atccgtactc ttcgtgcgca ggcaagagaa tgtacacttg
     6601 aaacgctgga agaaatgctg gaaaaattag aagttgttgt taacgaacgt cgcgaagaag
     6661 aaagcgcggc tgctgctgaa gttgaagagc gcactcgtaa actgcagcaa tatcgcgaaa
     6721 tgctgatcgc tgacggtatt gacccgaacg aactgctgaa tagccttgct tccgttaaat
     6781 ctggcaccaa agctaaacgt gctcagcgtc cggcaaaata tagctacgtt gacgaaaacg
     6841 gcgaaactaa aacctggact ggccaaggcc gtactccagc tgtaatcaaa aaagcaatgg
     6901 atgagcaagg taaatccctc gacgatttcc tgatcaagca ataatctttt gtagattgca
     6961 cttgcttaaa atcccgccag cggcgggatt ttttattgtc cggtttaaga caatttaata
     7021 agaataagct ataaaaaaac ggcatcgatt gctcaacgcc gtttcgtgga taacaccgat
     7081 acggatgtta cttcttaatg cccatctctt cttcaagcca ggctttaaat tccgtgccaa
     7141 gggtgttatg acgaataccg tattcaacga aggcctgcat gtaacctaat ttattaccgc
     7201 agtcatggct cttccctttc atatgatagg cttccaccgt ttctttttca atcagcatat
     7261 caattgcgtc ggtgagctga atttcatcac cagctcccgg aggggttttt gccagcaacg
     7321 gccaaatatc cgcgctaagt acgtaacgac ccacaatagc gagattagac ggcgcaacat
     7381 ccgctttcgg tttttctacc acaccaacca tcggtacgct ttcacccggc gctaattcaa
     7441 cgcctttgca atccacaacg ccatatgcgg tcacatcagc aaccggttca accatgatct
     7501 ggctatgacc cgtttcatca aagcggcgga tcatctctgc caggttatcc tgtgacaaat
     7561 cggattcata ttcatccaga ataacatcag gcaaaataac agctaccggt tcatcaccca
     7621 ctaccgggtg agcacacaat accgcgtgtc ccaggccttt cgccagaccc tgacgaactt
     7681 gcataatagt cacgtgcggt ggacaaatag actgcacttc atcaagcagt tgacgtttta
     7741 cacgtttttc cagcattgct tccagttcaa aactggtatc aaagtggttt tcaatagagt
     7801 ttttagatga gtgtgtaacc agcacaattt cagtaatgcc agccgcaata cattcattca
     7861 cgacgtattg aattaatggc ttatcgacaa gtggcagcat ctctttcggg atggctttcg
     7921 tcgccggcaa catcctggtt cctaatcccg caacggggat aacggctttt ttgactttcg
     7981 tattaatggc agccatttaa attctcctgg actgttcgtg ttttgaacgt gttcatattt
     8041 ctgtatcgca tcccagtata tcagcacccc gaaggggcta agttctgaaa aggaggcgag
     8101 ttagcataat taagacaatt acttatacga atggcgacga tagtaccacc agccgacatt
     8161 agcaggtaat gcaaatttag cccgcgttat cgtttgctca ttctgcagac aacatcaagc
     8221 gcagtcgacc accggttccc catatttggc attgccaggc atcgcatcgc tggctcaatt
     8281 gattcaaata agcgttacct aaagtgccta acggaacacc attactgatt tgcacctggt
     8341 gttcgccggt atttaacgtc gcattcagac ccgcagaaac cagaatgaga tttttcagtt
     8401 cgcgatgata atagccaact aatagcggaa actgccccgg cagattggcc tgacgaagta
     8461 aatggtttac ctgcttcaat aacgcgccca actctggcaa ccgttgattt tggtgtgcaa
     8521 gctgttcctg taataatccg ttaaacaatg cgcgtaataa caaggcagca agtacgccat
     8581 tatgtccagc tcgggtgaca tcaaggcaat aaaatgccag atcgttttcc gaaagtgcgg
     8641 caatatcaag caccaggccg ggtttgtccg cggcaaccaa ttgacgataa ttaacccggc
     8701 aatgggaaat cacctgctga accggcggtt gtagttcctg taataatttc gccgctgcgg
     8761 cagggttatc aaccattgca tcccagtcgc gaaaaagcct ttcctcttcc tcaacgcgcg
     8821 aattaaacat gctgggatag agacaggcaa aaaccatctc gcgcaagcga ttcaaatctt
     8881 taactggttt cagcaaaaca tcttcaacgc ccagacgtaa cgctttggca atatctgcca
     8941 tattttcggt ggcagatatc accagaaccg gtgtctggtc acctctgttg cgtatatgtt
     9001 ccagcagttt aagcccgttc attcgtggca tcgcgatatc acatatcatc aggtctggag
     9061 tgaaacctcc cagcaactca agggcatcca ccccatcagc cgccagtacc gttgtcgctc
     9121 ccaatgagga aaaccacgaa tccagaagcg agcgaaatac cggctcatct tcaacaatga
     9181 gaatctgttt tccgaccaat ggctgcgtca tgttctctcc cctgactggc tttactcaat
     9241 agtggcatgc tattgccacc tgcgcctgtc agaattaact taagtgtaga taaaaattct
     9301 ggtcaaatgt tggtgcgtac caacggcaaa agttcgtcca ttttcttttc cactgccagc
     9361 tgtccggccg caatggcagc gtgcgcgcga tggaaatcaa gcgtagatat ttgcgggcaa
     9421 acaggttgaa tcagaatatc gggcggatca cctgccatgc ggttcctttt aaggcggttc
     9481 tccagcacct ggatagaagt ggtcataatc tctgttgccg ttggcgctgt caccgcccga
     9541 cgtgtcgtta tgctgcctaa cctttctttc agacgcgcat gccacggcag agaatcacca
     9601 ttctcgctat tttcttcact gacattaaag gagagcaaat cttgttgcat caaatgagca
     9661 tcgtgctgca ggtcaaccgc tatcacaata tcagccccca atgcacgcgt gagggaaata
     9721 ggaattgggt taacgactgc tccatcaacc agccagtagc cgttatgtgc aacaggtgcc
     9781 atgagtcctg gaatactgca tgatgcgcga atagcaagat ggagatcgcc ttcagtaaac
     9841 cataattcac gtcccgtact taaattggtg gcaacagccg caaagcgacg ggaacaattt
     9901 tcgatctctg tttccggcat tatttcgcgg tattgattga agacacgctc gccgcgcagt
     9961 aacccaccgc gctgccagga gagatccatc aggcgtaaaa catcccaata actgaaagag
    10021 gtcacccaat cttccagcgc agataatcga tcgcatgcat aggcagcgcc caccagcgaa
    10081 ccaattgaac atcctgcaac gatatcaatt tcaataccca ctttttttag cgcattaata
    10141 acgccaatat gcgaccaacc tctcgccgcg ccagatccca gcgccagccc tatttttatc
    10201 tttctcatta ttcccgcaag attcccctgg aatgcaatcg tagccacatc gctactgctc
    10261 ggttaacata gtgctactca ggcggttaat acgccgttat tgtttccagg gagattattt
    10321 gtgtctcagc tttgtccctg tggtagtgct gtcgagtata gcctatgttg ccacccttat
    10381 gtgtctggtg aaaaggttgc acctgatcca gaacatctca tgcgttcgcg ttactgcgct
    10441 tttgtgatgc aagacgcaga ttatttaata aagacctggc atccctcttg tggagcagcg
    10501 gcgttacgtg ccgaattgat ggccggattt gcgcatactg aatggttggg attgaccgta
    10561 tttgaacatt gttggcagga cgccgacaat atcggatttg tcagttttgt tgctcgattt
    10621 accgaaggtg gtaaaacagg tgcaataatt gaacgttcgc gatttttgaa agaaaacggt
    10681 caatggtact atattgacgg tacacgtccg cagtttggtc gcaacgatcc ctgcccttgt
    10741 ggttcaggta aaaaatttaa aaagtgctgc ggccaataat ggttgacggt acggtttagc
    10801 aaacactctc aacaaggttt ttccagcaat gcattcactc caacgtaaag ttctgcgtac
    10861 tatttgtccg gaccaaaaag gtctgatcgc acgtattacc aatatttgct acaagcacga
    10921 gttaaatatc gtacagaaca atgaatttgt tgatcaccgt accgggcgct tttttatgcg
    10981 cacggaactg gaagggattt ttaatgattc caccctgctg gcggatctcg atagcgcatt
    11041 gccagaaggc tccgtgcgtg agctgaatcc tgccggtcgt cgccggatag tgattctggt
    11101 cactaaagaa gcgcattgcc ttggcgattt gttgatgaaa gccaattacg gcggcctgga
    11161 tgtcgaaatc gcggcagtta ttggtaacca cgatacttta cgttctctgg ttgagcgttt
    11221 tgatattccg tttgagctgg taagccatga agggttaagc cgcaacgagc acgatcaaaa
    11281 gatggcggac gccattgatg cttatcaatc tgactatgtg gtgctggcga agtatatgcg
    11341 ggtattaacg ccggaatttg tggcacgctt cccgaataaa atcatcaata ttcaccattc
    11401 attcctgcct gcgtttatcg gcgcacgtcc ttatcaccag gcctatgaac gtggtgtgaa
    11461 gattattggc gcaaccgctc actatgtgaa tgacaatctg gacgaaggcc caatcatcat
    11521 gcaggacgtt attcatgtcg atcataccta cacagctgaa gatatgatgc gcgcaggtcg
    11581 tgacgtcgag aaaaacgtct taagtcgtgc gctatacaaa gtactggcac agcgcgtctt
    11641 tgtttacggt aatcggacga ttattcttta atcgccagca aaaataactg gttaccttta
    11701 atccgttacg gatgaaaatt acgcaaccag ttcatttttc tcaacgtaac actttacagc
    11761 ggcgcgtcat ttgatatgat gcgccccgct tcccgataag ggagcaggcc agtaaaaagc
    11821 attaccccgt ggtggggttc ccgagcggcc aaagggagca gactgtaaat ctgccgtcat
    11881 cgacttcgaa ggttcg
//
LOCUS       DAZKHF020000132        11780 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2201S31241
            SAMN43267800-rid25106413.denovo.133, whole genome shotgun sequence.
ACCESSION   DAZKHF020000132 DAZKHF020000000
VERSION     DAZKHF020000132.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267800
            Sequence Read Archive: SRR30307263
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 11780)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11780)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 11780)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350581.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350581.2
            Genome Coverage       :: 151x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:21:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,652
            CDSs (total)                      :: 5,557
            Genes (coding)                    :: 5,320
            CDSs (with protein)               :: 5,320
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 237
            CDSs (without protein)            :: 237
            Pseudo Genes (ambiguous residues) :: 0 of 237
            Pseudo Genes (frameshifted)       :: 102 of 237
            Pseudo Genes (incomplete)         :: 130 of 237
            Pseudo Genes (internal stop)      :: 52 of 237
            Pseudo Genes (multiple problems)  :: 41 of 237
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11780
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267800-rid25106413.denovo.133"
                     /serotype="O 26:H11"
                     /isolate="2201S31241"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-01"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            complement(<1..677)
                     /locus_tag="ACHOSC_RS14780"
                     /old_locus_tag="pgaptmp_002956"
     CDS             complement(<1..677)
                     /locus_tag="ACHOSC_RS14780"
                     /old_locus_tag="pgaptmp_002956"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415949.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-guided endonuclease InsQ/TnpB family
                     protein"
                     /protein_id="HGU4548858.1"
                     /translation="MKRLQAFKFQLRPGGQQECEMRRFAGACRFVFNRALARQNENHE
                     AGNKYIPYGKMASWLVEWKNATETQWLKDSPSQPLQQSLKDLERAYKNFFRKRAAFPR
                     FKKRGQNDAFRYPQGVKLDQENSRIFLPKLGWMRYRNSRQVTGVVKNVTVSQSCGKWY
                     ISIQTESEVSTPVHPSASMIGLDAGVAKLATLSDGTVFGPVNSFQKNQKTLARLQRQL
                     SRKVKFS"
     gene            685..1116
                     /gene="tnpA"
                     /locus_tag="ACHOSC_RS14785"
                     /old_locus_tag="pgaptmp_002957"
     CDS             685..1116
                     /gene="tnpA"
                     /locus_tag="ACHOSC_RS14785"
                     /old_locus_tag="pgaptmp_002957"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605 family transposase"
                     /protein_id="HGU4548859.1"
                     /translation="MILWSVKKETDIRRGRHCVFLMHVHLVFVTRYRLQIFDHDATEK
                     LRTYFSNVCADFEAELVEMDGEPDHVHLLINYPPKLAISSLVNSLKGVSGRLLRRDRP
                     DIAVRYYYKGVLWSPGYFASSCGGAPISVIRQYIEQQQTPG"
     gene            complement(1132..1320)
                     /locus_tag="ACHOSC_RS14790"
                     /old_locus_tag="pgaptmp_002958"
     CDS             complement(1132..1320)
                     /locus_tag="ACHOSC_RS14790"
                     /old_locus_tag="pgaptmp_002958"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001569941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1869 domain-containing protein"
                     /protein_id="HGU4548860.1"
                     /translation="MGKATYTVTVTNNSNGVSVDYETETPMTLLVPEVAAEVIKDLVN
                     TVRSYPVRRKTPSFRAGI"
     gene            complement(1324..1683)
                     /gene="yeaR"
                     /locus_tag="ACHOSC_RS14795"
                     /old_locus_tag="pgaptmp_002959"
     CDS             complement(1324..1683)
                     /gene="yeaR"
                     /locus_tag="ACHOSC_RS14795"
                     /old_locus_tag="pgaptmp_002959"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1971 domain-containing protein YeaR"
                     /protein_id="HGU4548861.1"
                     /translation="MLQIPQNYIHTRSTPFWNKQTAPAGIFERHLDKGTRPGVYPRLS
                     VMHGAVKYLGYADEHSAEPDQVILIEAGQFAVFPPEKWHNIEAMTDDTYFNIDFFVAP
                     EVLMEGAQQRKVIHNGK"
     gene            complement(1856..2494)
                     /gene="leuE"
                     /locus_tag="ACHOSC_RS14800"
                     /old_locus_tag="pgaptmp_002960"
     CDS             complement(1856..2494)
                     /gene="leuE"
                     /locus_tag="ACHOSC_RS14800"
                     /old_locus_tag="pgaptmp_002960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="leucine efflux protein LeuE"
                     /protein_id="HGU4548862.1"
                     /translation="MFAEYGVLNYWTYLVGAIFIVLVPGPNTLFVLKNSVSSGMKGGY
                     LAACGVFIGDAVLMFLAWAGVATLIKTTPILFNIVRYLGAFYLLYLGSKILYATLKGK
                     NNEAKSDEPQYGAIFKRALILSLTNPKAILFYVSFFVQFIDVNAPHTGISFFILATTL
                     ELVSFCYLSFLIISGAFVTQYIRTKKKLAKVGNSLIGLMFVGFAARLATLQS"
     gene            complement(2621..3544)
                     /gene="dmlR"
                     /locus_tag="ACHOSC_RS14805"
                     /old_locus_tag="pgaptmp_002961"
     CDS             complement(2621..3544)
                     /gene="dmlR"
                     /locus_tag="ACHOSC_RS14805"
                     /old_locus_tag="pgaptmp_002961"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310535.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator DmlR"
                     /protein_id="HGU4548863.1"
                     /translation="MNNLPLLNDLRVFMLVARRAGFAAVAEELGVSPAFVSKRIALLE
                     QTLNVVLLHRTTRRVTITEEGERIYEWAQRILQDVGQMMDELSDVRQVPQGMLRIISS
                     FGFGRQVVAPALSALAKAYPQLELRFDVEDRLVDLVNEGVDLDIRIGDDIAPNLIARK
                     LATNYRILCASPEFIAQHGAPKHLTDLSALPCLVIKERDHPFGVWQLRNKEGHHAIKV
                     TGPLSSNHGEIVHQWCLDGQGIALRSWWDVSENIASGHLVQVLPEYYQPANVWAVYVS
                     RLATSAKVRITVEFLRQYFAEHYPNFSLEHA"
     gene            3647..4732
                     /gene="dmlA"
                     /locus_tag="ACHOSC_RS14810"
                     /old_locus_tag="pgaptmp_002962"
     CDS             3647..4732
                     /gene="dmlA"
                     /locus_tag="ACHOSC_RS14810"
                     /old_locus_tag="pgaptmp_002962"
                     /EC_number="1.1.1.83"
                     /EC_number="1.1.1.85"
                     /EC_number="1.1.1.93"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multifunctional
                     D-malate/3-isopropylmalate/tartarate dehydrogenase"
                     /protein_id="HGU4548864.1"
                     /translation="MMKTMRIAAIPGDGIGKEVLPEGIRVLQAAAERWGFALSFEQME
                     WASCEYYSHHGKMMPDDWHEQLSRFDAIYFGAVGWPDTVPDHISLWGSLLKFRREFDQ
                     YVNLRPVRLFPGVPCPLAGKQPGDIDFYVVRENTEGEYSSLGGRVNEGTEHEVVIQES
                     VFTRRGVDRILRYAFELAQSRPRKTLTSATKSNGLAISMPYWDERVEAMAENYPEIRW
                     DKQHIDILCARFVMQPERFDVVVASNLFGDILSDLGPACTGTIGIAPSANLNPERTFP
                     SLFEPVHGSAPDIYGKNIANPIATIWAGAMMLDFLGNGDERFQQAHNGILAAIEEVIA
                     HGPKTPDMKGNATTPQVADAICKIILR"
     gene            4983..6593
                     /gene="yeaV"
                     /locus_tag="ACHOSC_RS14815"
                     /old_locus_tag="pgaptmp_002963"
     CDS             4983..6593
                     /gene="yeaV"
                     /locus_tag="ACHOSC_RS14815"
                     /old_locus_tag="pgaptmp_002963"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310537.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BCCT family transporter YeaV"
                     /protein_id="HGU4548865.1"
                     /translation="MMSNVKKKDVPLISISLVAILFIAAALSLFPQQSADAANAIYTF
                     VTRTLGSAVQVLVLLAMGLVIYLATSKYGNIRLGEGKPEYSTLSWLFMFICAGLGSST
                     LYWGVAEWAYYYQTPGLNIAPRSQQALEFSVPYSFFHWGISAWATYTLASLIMAYHFH
                     VRKNKGLSLSGIIAAITGVRPQGPWGKLVDLMFLIATVGALTISLVVTAATFTRGLSA
                     LTGLPDNFTVQAFVILLSGGIFCLSSWIGINNGLQRLSKMVGWGAFLLPLLVLIVGPT
                     EFITNSIINAIGLTTQNFLQMSLFTDPLGDGSFTRNWTVFYWLWWISYTPGVAMFVTR
                     VSRGRKIKEVIWGLILGSTVGCWFFFGVMESYAIHQFINGVINVPQVLETLGGETAVQ
                     QVLMSLPAGKLFLAAYLGVMIIFLASHMDAVAYTMAATSTRNLQEGDDPDRGLRLFWC
                     VVITLIPLSILFTGASLETMKTTVVLTALPFLVILLVKVGGFIRWLKQDYADIPAHQV
                     EHYLPQTPVEALEKTPVLPAGTVFKGDN"
     gene            6625..7749
                     /gene="yeaW"
                     /locus_tag="ACHOSC_RS14820"
                     /old_locus_tag="pgaptmp_002964"
     CDS             6625..7749
                     /gene="yeaW"
                     /locus_tag="ACHOSC_RS14820"
                     /old_locus_tag="pgaptmp_002964"
                     /EC_number="1.14.13.239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carnitine monooxygenase, oxygenase subunit YeaW"
                     /protein_id="HGU4548866.1"
                     /translation="MSNLSPDFVLPENFCANPQEAWTIPARFYTDQNAFEHEKENVFA
                     KSWICVAHSIELANANDYVTREIIGESIVLVRGRDKVLRAFYNVCPHRGHQLLSGEGK
                     AKNVITCPYHAWAFKLDGNLAHARNCENVANFDSDKAQLVPVRLEEYAGFVFINMDPN
                     ATSVEDQLPGLGAKVLEACPEVHDLKLAARFTTRTPANWKNIVDNYLECYHCGPAHPG
                     FSDSVQVDRYWHTMHGNWTLQYGFAKPSEQSFKFEEGTDAAFHGFWLWPCTMLNVTPI
                     KGMMTVIYEFPVDSETTLQNYDIYFTNEELTDEQKSLIEWYRDVFRPEDLRLVESVQK
                     GLKSRGYRGQGRIMADSSGSGISEHGIAHFHNLLAQVFKD"
     gene            7805..8770
                     /gene="yeaX"
                     /locus_tag="ACHOSC_RS14825"
                     /old_locus_tag="pgaptmp_002965"
     CDS             7805..8770
                     /gene="yeaX"
                     /locus_tag="ACHOSC_RS14825"
                     /old_locus_tag="pgaptmp_002965"
                     /EC_number="1.14.13.239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carnitine monooxygenase reductase subunit YeaX"
                     /protein_id="HGU4548867.1"
                     /translation="MSDYQMFEVQVSQVEPLTEQVKRFTLVATDGKPLPAFTGGSHII
                     VQMSDGDNQYSNAYSLLSSPHDTSCYQIAVRLEENSRGGSRFLHQQVKVGDRLTISTP
                     NNLFALIPSARKHLFIAGGIGITPFLSHMAELQHSDVDWQLHYCSRNPESCAFRDELV
                     QHPQAEKVHLHHSSTGTRLELARLLADIEPGTHVYTCGPEALNEAVRSEAARLDIVAD
                     TLHFEQFAIEDKTGDAFTLVLARSGKEFVVPEEMTILQVIENNKAAKVECLCREGVCG
                     TCETAILEGEADHRDQYFSDEERASQQSMLICCSRAKGKRLVLDL"
     gene            complement(8824..9939)
                     /gene="rnd"
                     /locus_tag="ACHOSC_RS14830"
                     /old_locus_tag="pgaptmp_002966"
     CDS             complement(8824..9939)
                     /gene="rnd"
                     /locus_tag="ACHOSC_RS14830"
                     /old_locus_tag="pgaptmp_002966"
                     /EC_number="3.1.13.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310540.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_function: GO:0033890 - ribonuclease D activity
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease D"
                     /protein_id="HGU4548868.1"
                     /translation="MITTDDALASLCEAVRAFPAIALDTEFVRTRTYYPQLGLIQLFD
                     GEHLALIDPLGITDWSPLKAILRDPSITKFLHAGSEDLEVFLNVFGELPQPLIDTQIL
                     AAFCGRPMSWGFASMVEEYSGVTLDKSESRTDWLARPLTERQCEYAAADVWYLLPITA
                     KLMVETEASGWLPAALDECRLMQMRRQEVVAPEDAWRDITNAWQLRTRQLACLQLLAD
                     WRLRKARERDLAVNFVVREEHLWSVARYMPGSLGELDSLGLSGSEIRFHGKTLLALVE
                     KAQTLPEEALPQPMLNLMDMPGYRKVFKAIKSLITDVSETHKISAELLASRRQINQLL
                     NWHWKLKPQNNLPALISGWRGELMAEALHNLLQEYPQ"
     gene            complement(10021..11706)
                     /gene="fadD"
                     /locus_tag="ACHOSC_RS14835"
                     /old_locus_tag="pgaptmp_002967"
     CDS             complement(10021..11706)
                     /gene="fadD"
                     /locus_tag="ACHOSC_RS14835"
                     /old_locus_tag="pgaptmp_002967"
                     /EC_number="6.2.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long-chain-fatty-acid--CoA ligase FadD"
                     /protein_id="HGU4548869.1"
                     /translation="MKKVWLNRYPADVPTEINPDRYQSLVDMFEQSVARYADQPAFVN
                     MGEVMTFRKLEERSRAFAAYLQQGLGLKKGDRVALMMPNLLQYPVALFGILRAGMIVV
                     NVNPLYTPRELEHQLNDSGASAIVIVSNFAHTLEKVVDKTAVQHVILTRMGDQLSTAK
                     GTVVNFVVKYIKRLVPKYHLPDAISFRSALHNGYRMQYVKPELVPEDLAFLQYTGGTT
                     GVAKGAMLTHRNMLANLEQVNATYGPLLHPGKELVVTALPLYHIFALTINCLLFIELG
                     GQNLLITNPRDIPGLVKELAKYPFTAITGVNTLFNALLNNKEFQQLDFSSLHLSAGGG
                     MPVQQVVAERWVKLTGQYLLEGYGLTECAPLVSVNPYDIDYHSGSIGLPVPSTEAKLV
                     DDDDNEVPPGQPGELCVKGPQVMLGYWQRPDATDEIIKNGWLHTGDIAVMDEEGFLRI
                     VDRKKDMILVSGFNVYPNEIEDVVMQHPGVQEVAAVGVPSGSSGEAVKIFVVKKDPSL
                     TEESLVTFCRRQLTGYKVPKLVEFRDELPKSNVGKILRRELRDEARGKVDNKA"
BASE COUNT         2895 a         3053 c         2874 g         2958 t
ORIGIN      
        1 ttgctgaatt tgaccttgcg gcttaactgt cgctgaagtc tcgccagcgt cttctggttt
       61 ttctgaaaac tgtttacagg cccaaagact gtgccatctg acagcgtggc gagtttagcc
      121 acgccagcat ccagcccgat cattgatgct gaagggtgaa ccggagttga tacttcactt
      181 tctgtctgaa tactgatgta ccacttaccg caggactggc tgaccgtgac atttttcaca
      241 acacccgtga cctgacggct attccggtag cgcatccagc ccagtttcgg cagaaaaata
      301 cggctgtttt cctgatcgag ctttacgccc tgcgggtagc ggaatgcatc attctgtccc
      361 cgctttttga atcggggaaa agcagcccgc ttccggaaga agtttttgta agcccgctca
      421 aggtctttca gtgactgttg caatggctgt gagggcgaat ctttaagcca ttgcgtttca
      481 gtggcgtttt tccactcaac cagccaggaa gccattttcc cgtaagggat gtatttatta
      541 ccggcctcat gattctcatt ctgacgtgcc agagcacgat tgaaaacgaa acgacatgcg
      601 cctgcgaagc gcctcatttc acactcctgt tgaccacctg gtcttaactg gaatttaaat
      661 gcctgtaatc gtttcacagg tcaaatgata ctttggtctg tgaaaaaaga aaccgatatc
      721 cggcgtggaa ggcattgtgt tttcctgatg catgttcacc tggtctttgt caccagatac
      781 cgactccaga tttttgacca tgacgcgaca gaaaaactac gcacttactt ttcaaatgta
      841 tgtgctgatt ttgaagctga acttgttgaa atggatggcg aaccagatca cgtccatttg
      901 ttaattaact atcctcccaa actggcgata tccagtctgg taaacagcct caaaggcgta
      961 tcgggtaggt tactgcgacg agatcgacca gatattgcag tcaggtatta ctacaaaggc
     1021 gttttgtgga gtcctggcta ttttgccagt agctgcggag gtgcgccaat atccgtcatc
     1081 cgccaataca ttgaacaaca gcaaacacct ggttagatgg aaaaccgcgc cttatatccc
     1141 cgccctgaag gacggggttt tacggcgcac cggataagaa cgcacggtat tcaccagatc
     1201 ttttatcact tcagccgcca cttctggcac cagcaaagtc atcggcgtct ctgtttcata
     1261 atcgacagaa acgccattgc tgttattggt gacggtcacg gtatacgttg ctttgcccat
     1321 gattcatttc ccgttatgaa tgactttccg ttgttgcgca ccttccatca ggacttcagg
     1381 agccacgaag aagtcaatgt tgaaataagt atcgtcagtc atggcttcaa tgttgtgcca
     1441 cttttctgga gggaacaccg caaactgccc cgcttcgata aggatcacct gatcaggctc
     1501 tgcactgtgt tcatcagcgt agccgagata tttgaccgcc ccatgcataa cggaaaggcg
     1561 tgggtaaacc cccgggcgcg ttcctttatc aagatgacgt tcgaatattc cggcaggcgc
     1621 agtttgttta ttccagaaag gcgttgagcg cgtatgaata taattctgtg ggatttgcag
     1681 catcctattc cctccttcgg ggaatgcgct gaaaacggtt tattccagcc gtttcagggt
     1741 acgcctgata atttgcattt taaataccat ttattggtta ctttttagca ccatatcaga
     1801 gaaaaatcag ggaggattac aagtggggag cccgcgacaa cgcgggctga atgtatcagg
     1861 attgcagcgt cgccagtcgg gcggcgaaac ccacgaacat caaaccaatc agtgagttgc
     1921 caactttagc cagtttcttt ttggtacgta tgtactgcgt gacaaaagca ccagaaataa
     1981 tcaggaagct caaatagcag aaactcacca gttccagcgt cgtcgccaga ataaagaatg
     2041 aaattcccgt atgtggggca ttaacatcga taaactgtac gaaaaacgac acatagaaca
     2101 aaatggcttt cggattagtc aggctcaaaa ttaacgcgcg tttaaaaatg gccccgtatt
     2161 ggggctcatc ggatttggcc tcgttatttt tacccttcag ggtcgcgtag agaattttac
     2221 tccccagata gagcaaataa aacgcaccaa gataacgtac aatgttgaat aatatcgggg
     2281 tggtcttaat taatgtcgcc actccagccc atgccagaaa catcaatact gcatcgccaa
     2341 taaatacacc gcaggccgca agataaccgc ctttcatacc gctactgacg ctatttttga
     2401 gtacaaacag ggtatttggc cctggcacca acacaataaa aatggcccca accagatagg
     2461 tccagtaatt cagaaccccg tattcagcga acacattaac ctctttaatt atctttcgat
     2521 catgcgcgat taaaggtgaa tatgctaacc aatctgtagc ggcttagaaa ggagaaaatc
     2581 aggttttaac ccgatatcaa cccgataatt gaatcattaa tcaggcatgc tccagtgaaa
     2641 aattcgggta gtgctcggca aaatactggc gtaaaaattc taccgttatc cgcactttcg
     2701 ctgacgtcgc cagccttgaa acataaacgg cccagacgtt cgctggctgg taatattccg
     2761 gtagcacttg cactaaatga ccactggcaa tgttttcgct aacatcccac caggagcgca
     2821 gagcaatccc ctgcccatcg agacaccact gatggacaat ttctccatga ttagacgaca
     2881 gtgggccggt gactttaatc gcatggtggc cttctttatt gcgtaattgc cagacaccaa
     2941 aaggatgatc gcgctcttta atgaccaggc acggcaatgc ggaaagatcg gttaaatgtt
     3001 tcggcgcgcc atgttgcgca ataaattccg gcgacgcgca gagaatgcgg taattagtcg
     3061 ccagtttgcg ggcgattaaa ttaggggcga tgtcatcgcc aatgcgaata tcgagatcaa
     3121 caccttcatt gaccaaatcg accaaccggt cttccacatc aaaacgtaat tccagttgcg
     3181 gatacgcctt agccagcgct gataacgccg gagccaccac ctgtcgtcca aaaccaaagc
     3241 tgctgataat acgcagcatc ccctgcggca cctgacgcac gtcagaaagt tcgtccatca
     3301 tctgaccgac atcctgcaaa atccgctgcg cccattcata aatccgctct ccttcttcgg
     3361 taatggtgac gcggcgggtg gtgcggtgta acaacaccac gtttagcgtt tgctccagca
     3421 aggcgatgcg cttgctgacg aacgccggtg aaacgcccag ttcttccgcc acggcggcaa
     3481 aaccagcccg acgagccacc agcataaaga cgcgcaaatc attcagcagc ggtaaattat
     3541 tcatgattcg tgttttatgt ttcaccagtt acggggatta attccttttc agtcaattat
     3601 aggatggtga tgttgtcaat tttgatggtc aggaagtgag aacccaatga tgaaaacgat
     3661 gcgtattgct gcgatcccgg gagacgggat tggcaaagaa gtccttcctg aagggattcg
     3721 cgtgttacag gctgccgctg agcgctgggg cttcgccttg agttttgagc aaatggagtg
     3781 ggcgagctgc gagtattaca gccatcacgg taaaatgatg ccggacgact ggcatgagca
     3841 acttagccgt ttcgacgcca tctattttgg tgccgtcggc tggccggaca ccgttcctga
     3901 ccatatttcg ttgtggggtt cgctgctgaa atttcgtcgt gaattcgacc agtacgtcaa
     3961 cctgcgcccg gttcgtctct ttcctggcgt tccctgcccg ctggcgggaa aacagcctgg
     4021 cgacatcgat ttttacgtgg tcagggaaaa caccgaaggc gaatattcct cgctcggcgg
     4081 tagagtgaat gaaggtacag agcatgaagt cgtcattcag gaatcggtat ttacgcgtcg
     4141 tggtgtcgat cgcattttgc gttatgcctt cgaacttgcg caaagccgcc cacgtaagac
     4201 gctaacttct gccactaaat caaacggttt agccatcagc atgccgtact gggatgagcg
     4261 agtggaagca atggccgaga attacccgga gatccgctgg gacaagcagc atattgatat
     4321 tctctgcgcg cgttttgtga tgcagccgga acgcttcgat gtggtggtgg cgtccaattt
     4381 atttggcgat atcctttccg atcttggccc ggcctgcacc ggcaccattg gcattgcccc
     4441 atccgccaac ctgaatccgg aacgcacttt cccgtcgctc ttcgagccag tccacggttc
     4501 cgcgccggat atctacggga aaaacattgc taaccctatc gccacgattt gggccggtgc
     4561 aatgatgctc gattttctcg gcaatggcga tgagcgtttc cagcaagcgc ataacggtat
     4621 tctggcagca attgaagaag tgattgctca cgggccgaaa acacctgata tgaaaggcaa
     4681 tgccaccacg ccacaggttg ccgacgcgat ttgcaaaatt attttgcgtt aaggtcaaac
     4741 cagtttattt gaaccgcgtc actgacgcgg tttttttatt cgttctttgc agtaaataac
     4801 ctgcgtcatt tcacctttta ttgtttccgt ttcgtgtttt atggctttcc gtattcttaa
     4861 ttgtttaatt tatgtaacat gaaaataatc aatttgttaa acacaacata caactcatta
     4921 ccctgcaaat aattacaact ggcaaaagcc agagacgggc ttttatgccc aggagatgac
     4981 tcatgatgag caatgttaag aaaaaagatg tgccgctgat aagcatcagc ctggtggcca
     5041 ttcttttcat cgcagctgca ttaagccttt tcccacaaca atcggccgac gcggccaacg
     5101 caatatacac ttttgttacg cgtacgttag gttccgccgt acaggtattg gttttgctgg
     5161 caatgggact ggtgatttat ttagccacca gtaaatacgg caatattcgt cttggcgaag
     5221 gaaaaccgga atacagcacg ctctcctggc tgtttatgtt tatttgtgcc ggtttaggtt
     5281 cttctacgct ttattggggg gttgctgaat gggcctatta ttatcaaacg cctggattaa
     5341 atatcgcacc gcgttcacaa caggcactcg aatttagcgt cccttactcc ttcttccact
     5401 ggggcatcag cgcctgggca acttatacgc tggcctcatt aatcatggct tatcactttc
     5461 atgtgcgtaa aaacaaaggt ctgagtcttt ccggcattat tgccgcgatt accggcgttc
     5521 gcccgcaagg cccatgggga aaactggttg atttgatgtt cctgatcgcc actgtcggcg
     5581 cactgactat ttcccttgtt gtcaccgcag caacctttac tcgtggactt tccgcgctga
     5641 ccggtttacc cgataacttc accgtgcagg catttgtgat cctgctttcc ggcggcattt
     5701 tttgcctaag ctcgtggatt ggtatcaaca acggtttgca acgtctgagc aaaatggttg
     5761 gctggggcgc gttcctgctg ccattactgg tgctgattgt cggcccaacc gaatttatta
     5821 ccaacagcat catcaatgcc atcggtctga ccacgcaaaa cttcctgcaa atgagcttat
     5881 tcaccgatcc gcttggcgat ggttcattta cccgcaactg gaccgttttc tactggctgt
     5941 ggtggatctc atacacccct ggcgtagcaa tgtttgtcac ccgcgtttcc cgcggtcgta
     6001 agattaaaga agttatctgg ggactgatcc tcggcagcac cgtcggttgc tggttcttct
     6061 tcggcgtaat ggaaagctat gccattcatc agtttatcaa tggcgtaatc aacgtcccac
     6121 aggtgctgga aacactgggc ggcgaaacag ctgtacagca agttctgatg tcgttgccag
     6181 ccggtaaatt gttcctcgcc gcatacctgg gcgtgatgat tattttcctt gcctcgcata
     6241 tggatgcggt ggcctacacc atggctgcga ccagtacgcg taatctccag gaaggtgacg
     6301 atcctgaccg tgggctgcgt cttttctggt gcgtggtgat cactctgatc ccgctttcca
     6361 tcttgtttac cggtgcttcg ctggaaacga tgaaaaccac cgtcgtgctc acagcccttc
     6421 ccttcctcgt cattttactg gtgaaagtcg gcggatttat tcgctggctg aaacaggatt
     6481 acgccgacat tccggctcat caagttgaac attatctccc gcagacaccg gttgaagccc
     6541 tggaaaaaac gccagtgctc cctgcgggaa ccgtattcaa aggcgacaac tgagcgccat
     6601 catcctaacg ataaaggtat ccctatgagc aatctgagcc ctgactttgt actacccgaa
     6661 aatttttgcg ctaacccgca agaggcgtgg accattcctg cccgttttta taccgatcag
     6721 aacgcgtttg aacacgaaaa agagaacgtc ttcgccaaaa gctggatttg cgtcgctcac
     6781 agcattgaac tggcgaatgc caatgattat gtgacgcgtg agatcattgg cgaaagcatc
     6841 gtgctggtac gcggtcgtga taaggttttg cgcgcgttct ataacgtgtg tccgcaccgt
     6901 ggtcatcagt tgttgagcgg tgaaggaaaa gcaaaaaatg tgattacctg cccgtatcac
     6961 gcatgggcat tcaaactcga tggcaatctg gcccatgcac gtaactgcga aaacgtcgcc
     7021 aatttcgata gcgacaaagc gcaactggtt ccggtgcgtc tggaagaata tgccggattc
     7081 gtcttcatca acatggaccc caacgccacc agcgtagaag atcagttacc cggtctgggc
     7141 gcgaaagtgc tggaagcctg cccggaagtc cacgatctga aactggcggc ccgctttacc
     7201 acccgcacgc ctgccaactg gaagaacatt gtcgataact atctcgagtg ctatcactgt
     7261 ggtccggcgc atccaggttt ctccgactcc gtacaggttg atcgttactg gcacaccatg
     7321 cacggtaact ggacgctgca atacggtttc gccaaaccgt ccgaacagtc gtttaaattt
     7381 gaagagggta cggatgcggc attccacggt ttctggctgt ggccgtgcac gatgctgaac
     7441 gtcaccccga tcaaagggat gatgacggtc atttatgaat tcccggtgga ttctgaaact
     7501 accctgcaaa actacgatat ttacttcacc aatgaagagt taaccgacga gcaaaaatcg
     7561 ctgattgagt ggtatcgcga tgtgttccgt ccggaagatt tacgtctggt tgaaagcgta
     7621 cagaaagggc tgaaatcgcg tggctatcgt ggtcaggggc gcatcatggc cgacagtagc
     7681 ggtagtggca tttccgaaca tggtatcgcc catttccata atctgctggc gcaggtgttt
     7741 aaggactaat gacatcggcg gcggtatttt ccgccgctgg gctgattttt gatggagtac
     7801 agcaatgtca gactatcaaa tgtttgaagt acaggtgagt caggtggaac cgcttaccga
     7861 acaggtgaaa cgcttcacgc tggtggcaac cgatggcaaa ccgctgcctg cgttcaccgg
     7921 aggaagtcac atcattgtgc agatgagcga tggtgataac cagtacagca atgcgtattc
     7981 actactgagt tcgccgcatg acacctcttg ttatcagatt gccgttcggc tggaggaaaa
     8041 ctcgcgcggc ggttcccgct ttttgcatca gcaggtaaaa gtgggcgatc ggttaacgat
     8101 ttcaacgcct aataacctgt ttgcgctaat tccctcagcc agaaagcatc tgtttatcgc
     8161 gggcggtatt ggtatcaccc ctttcctgtc gcacatggca gagctgcaac acagcgacgt
     8221 cgactggcag ctacattact gctcgcgaaa tccagaaagt tgcgcatttc gtgatgagct
     8281 agtccagcat ccgcaggctg agaaagttca cttgcatcac tcatcaaccg gaacacgact
     8341 ggaattagcg cgattattgg cggatatcga acctggcaca cacgtttata cctgtggtcc
     8401 cgaggcgcta aatgaagcgg taagaagtga agctgcgcgt ctggacatcg tcgccgatac
     8461 gctgcacttt gagcaatttg ctatcgaaga caaaaccggc gatgccttta ccctggtgct
     8521 tgcccgttcc ggaaaagagt ttgtggtgcc ggaagagatg actattttgc aggttattga
     8581 aaataataaa gccgcgaaag tggaatgttt atgtcgtgaa ggggtatgcg gaacctgcga
     8641 aacagcaata ctggaaggtg aagctgacca tcgggatcag tattttagcg atgaagagcg
     8701 tgccagccag caaagtatgt tgatctgttg ttcgcgtgcg aagggtaaac gcctggtgtt
     8761 ggatttgtag tttgccaaag ccggatgtgg cgctgagcgc gcccagtccg gcttcggaag
     8821 attttactgc ggatattcct gcaataaatt gtgtaatgct tccgccatca gctcaccacg
     8881 ccagccggaa atcagcgccg gcaaattgtt ctgcggtttc agtttccagt gccagttcag
     8941 cagttggttg atttgccgac gcgatgccag caattcggcg ctgatcttat gcgtttcgct
     9001 cacgtcagta atcagcgact taatcgcttt aaacacttta cgataaccag gcatgtccat
     9061 caggttaagc atcggctgcg gtaaggcctc ttccggcaat gtctgcgctt tttccaccag
     9121 cgccagcagc gttttaccgt gaaagcggat ttcgctaccg gataaaccca ggctgtccag
     9181 ttcgcctaaa cttcccggca tataacgcgc gaccgaccac aaatgctctt cacgcacgac
     9241 aaagttcacc gccagatcac gctctcgcgc cttgcgcagt cgccagtcgg ctaacagttg
     9301 cagacaggcc agttggcgtg tgcgtaactg ccaggcattg gtgatatcac gccaggcatc
     9361 ttccggcgca acgacttcct gacgacgcat ttgcatcagg cggcattcat ccagcgccgc
     9421 aggtagccag ccggaggcct ccgtttctac cataagcttg gcggtgatcg gtaacagata
     9481 ccagacatcc gccgctgcgt attcacactg acgttcggtc agcggtctgg ccagccagtc
     9541 ggtgcgcgat tcactcttgt ccagcgtaac gccggaatac tcttccacca tggaagcgaa
     9601 accccatgac atcgggcgtc cgcagaaggc agcaaggatt tgcgtgtcaa tcaagggttg
     9661 tggtaattcg ccaaagacat tgaggaacac ttccagatct tcactgcctg catggagaaa
     9721 ttttgtgatg gacggatcgc gcaggatcgc tttcagcggt gaccagtcgg tgatcccgag
     9781 aggatcaatt agcgccagat gctcgccatc gaaaagttga atcaacccca gctgcgggta
     9841 ataagtgcgc gtacgaacaa attcagtatc cagggctatc gccggaaagg cacggacggc
     9901 ttcacacaaa gaagccagcg catcgtccgt ggtaatcatt tggtaattca aattgttttc
     9961 tctttagtgg gcgtcaaaaa aaacgccgga ttaaccggcg tctgacgact gacttaacac
    10021 tcaggcttta ttgtccactt tgccgcgcgc ttcgtcacgt aattctcgtc gcaaaatttt
    10081 tccgacgtta gatttcggta actcatcacg aaactccacc agcttcggta ctttgtagcc
    10141 cgtgagctga cggcggcaaa aggtcaccag tgactcttcg gtaagcgatg gatctttttt
    10201 cactacgaag attttcaccg cttcaccact ggagccggaa ggtacgccaa cagccgcgac
    10261 ttcctgtacg ccaggatgct gcatgacgac atcttcaatc tcgttgggat agacgttaaa
    10321 accggaaacc agaatcatgt cttttttacg atcgacaatg cgcaggaacc cttcttcatc
    10381 catcaccgcg atgtcgccgg tgtgtaacca gccatttttg atgatctcat ctgtagcatc
    10441 cggacgctgc cagtaaccca gcatcacctg cggtcctttg acacaaagct cacccggttg
    10501 acccggtggt acttcattat catcatcatc caccagtttg gcttccgtcg acggcaccgg
    10561 caaaccgatg ctaccactat gataatcaat atcatatggg ttaacgctga ccagcggcgc
    10621 acactcggta aggccatagc cttccagcag atactgtcct gtcagtttca cccaacgctc
    10681 tgccaccact tgctgcactg gcatccctcc gcctgcggaa agatgcagac tggagaaatc
    10741 cagctgctgg aactctttat tgttcagcaa cgcattgaac aaggtgttaa cgcccgtgat
    10801 agcggtaaac ggatatttcg ctaactcttt taccaaccct ggaatatcgc gcgggttagt
    10861 gataagcagg ttctgcccac ccagttcgat aaacagcagg cagttaatgg tcagggcaaa
    10921 aatgtgatac agcggcagcg ccgtcaccac cagctctttg cccggatgca acagcggacc
    10981 ataggtcgcg ttaacctgtt ccaggttcgc cagcatattg cggtgagtca gcatcgcgcc
    11041 tttcgccaca ccagtggtgc cgccggtgta ttgcagaaaa gctaaatctt ccggcaccag
    11101 ttcgggtttg acgtactgca tccggtagcc gttatgcagt gcgctacgaa atgaaatggc
    11161 atctggcaga tggtatttcg gcaccaaacg cttgatgtat ttaacaacga aattgactac
    11221 cgtgcctttt gccgtagata gctgatcgcc catacgggtc agaattacgt gctgaacggc
    11281 ggttttatca accacttttt ccagtgtgtg agcaaagtta gacacgataa caatcgccga
    11341 tgcgccgcta tcgttaagct gatgctcaag ctcacgcggg gtatacaacg ggttaacgtt
    11401 tacgacgatc atcccggcac gcaaaatgcc aaacagcgcc accggatatt gcaataaatt
    11461 aggcatcatc aacgcaacgc gatcgccttt cttcagcccc aacccttgtt gcaaataagc
    11521 ggcaaacgcg cgactgcgtt cttccagctt gcggaaggtc attacctccc ccatattcac
    11581 aaacgcaggt tgatcggcgt agcgcgcgac cgactgctca aacatatcta ccagagattg
    11641 ataacggtca gggttgatct ccgtcggaac gtccgcggga taacggttaa gccaaacctt
    11701 cttcaattct tcacctctaa aatgcgtgtt cgtcgtcatc gcaaccccaa atgatataca
    11761 tgccgttaac ataatattaa
//
LOCUS       DAZKHF020000133        11758 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2201S31241
            SAMN43267800-rid25106413.denovo.134, whole genome shotgun sequence.
ACCESSION   DAZKHF020000133 DAZKHF020000000
VERSION     DAZKHF020000133.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267800
            Sequence Read Archive: SRR30307263
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 11758)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11758)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 11758)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350581.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350581.2
            Genome Coverage       :: 151x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:21:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,652
            CDSs (total)                      :: 5,557
            Genes (coding)                    :: 5,320
            CDSs (with protein)               :: 5,320
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 237
            CDSs (without protein)            :: 237
            Pseudo Genes (ambiguous residues) :: 0 of 237
            Pseudo Genes (frameshifted)       :: 102 of 237
            Pseudo Genes (incomplete)         :: 130 of 237
            Pseudo Genes (internal stop)      :: 52 of 237
            Pseudo Genes (multiple problems)  :: 41 of 237
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11758
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267800-rid25106413.denovo.134"
                     /serotype="O 26:H11"
                     /isolate="2201S31241"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-01"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            complement(65..346)
                     /locus_tag="ACHOSC_RS14840"
                     /old_locus_tag="pgaptmp_002968"
     CDS             complement(65..346)
                     /locus_tag="ACHOSC_RS14840"
                     /old_locus_tag="pgaptmp_002968"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YehE family protein"
                     /protein_id="HGU4548870.1"
                     /translation="MNKYWLSGIIFLAYGLASPAFSSETATLAINGRISPPTCSMAMV
                     NGQPQQHCGQLTYNVDTRHLFSSPVKGVTTEVVVAGSDSKRRIVLNRYD"
     gene            complement(609..1718)
                     /gene="apbC"
                     /locus_tag="ACHOSC_RS14845"
                     /old_locus_tag="pgaptmp_002969"
     CDS             complement(609..1718)
                     /gene="apbC"
                     /locus_tag="ACHOSC_RS14845"
                     /old_locus_tag="pgaptmp_002969"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310946.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster carrier protein ApbC"
                     /protein_id="HGU4548871.1"
                     /translation="MNEQSQAKSPEALRAMVAGTLANFQHPTLKHNLTTLKALHHVAW
                     MDDTLHVELVMPFVWHSAFEELKEQCSAELLRITGAKAIDWKLSHNIATLKRVKNQPG
                     INGVKNIIAVSSGKGGVGKSSTAVNLALALAAEGAKVGILDADIYGPSIPTMLGAENQ
                     RPTSPDGTHMAPIMSHGLATNSIGYLVTDDNAMVWRGPMASKALMQMLQETLWPDLDY
                     LVLDMPPGTGDIQLTLAQNIPVTGAVVVTTPQDIALIDAKKGIVMFEKVEVPVLGIVE
                     NMSVHICSNCGHHEPIFGTGGAEKLAEKYHTQLLGQMPLHISLREDLDKGTPTVISRP
                     ESEFTAIYRQLADRVAAQLYWQGEVIPGEISFRAV"
     gene            1850..3883
                     /gene="metG"
                     /locus_tag="ACHOSC_RS14850"
                     /old_locus_tag="pgaptmp_002970"
     CDS             1850..3883
                     /gene="metG"
                     /locus_tag="ACHOSC_RS14850"
                     /old_locus_tag="pgaptmp_002970"
                     /EC_number="6.1.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004825 - methionine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006431 - methionyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine--tRNA ligase"
                     /protein_id="HGU4548872.1"
                     /translation="MTQVAKKILVTCALPYANGSIHLGHMLEHIQADVWVRYQRMRGH
                     EVNFICADDAHGTPIMLKAQQLGITPEQMIGEMSQEHQTDFAGFNISYDNYHSTHSEE
                     NRQLSELIYSRLKENGFIKNRTISQLYDPEKGMFLPDRFVKGTCPKCKSPDQYGDNCE
                     VCGATYSPTELIEPKSVVSGATPVMRDSEHFFFDLPSFSEMLQAWTRSGALQEQVANK
                     MQEWFESGLQQWDISRDAPYFGFEIPNAPGKYFYVWLDAPIGYMGSFKNLCDKRGDSV
                     SFDEYWKKDSTAELYHFIGKDIVYFHSLFWPAMLEGSNFRKPTNLFVHGYVTVNGAKM
                     SKSRGTFIKASTWLNHFDADSLRYYYTAKLSSRIDDIDLNLEDFVQRVNADIVNKVVN
                     LASRNAGFINKRFDGVLASELADPQLYKTFTDAAEVIGEAWESREFGKAVREIMALAD
                     LANRYVDEQAPWVVAKQEGRDADLQAICSMGINLFRVLMTYLKPVLPKLTERAEAFLN
                     TELTWDGIQQPLLGHKVNPFKALYNRIDMKQVEALVEASKEEVKAAAAPVTGPLADDP
                     IQETITFDDFAKVDLRVALIENAEFVEGSDKLLRLTLDLGGEKRNVFSGIRSAYPDPQ
                     ALIGRHTIMVANLAPRKMRFGISEGMVMAAGPGGKDIFLLSPDAGAKPGHQVK"
     gene            4024..7818
                     /locus_tag="ACHOSC_RS14855"
                     /old_locus_tag="pgaptmp_002971"
                     /pseudo
     CDS             4024..7818
                     /locus_tag="ACHOSC_RS14855"
                     /old_locus_tag="pgaptmp_002971"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001215568.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4132 domain-containing protein"
     gene            7828..11460
                     /gene="yehI"
                     /locus_tag="ACHOSC_RS14860"
                     /old_locus_tag="pgaptmp_002972"
     CDS             7828..11460
                     /gene="yehI"
                     /locus_tag="ACHOSC_RS14860"
                     /old_locus_tag="pgaptmp_002972"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4132 domain-containing protein YehI"
                     /protein_id="HGU4548873.1"
                     /translation="MDKELPWLADNAQLELKYKKGKTPLSHRRWPGEPVSVITGSLIQ
                     TLGDELLQQAGQKENITWNYDKCSLEWQSAIQQAINLTGEHKPSIPALTMAALICIAQ
                     NDSQQLLDEIVQQEGLEYATDVVIARQCIARRYESDSLVVTLQYQDEDYGYGYGSATY
                     NDFDLRLRKHLSLAEESCWQRCADKLIAALPGIPKIRRPFIALILPEKPEIANELASL
                     ESSRSSLHSKEWLKVVATDNTAVKKLERYWGLDVFSDREASYMSQENRFGYAACASLL
                     REQGLAAVPRLAMYAHKEDCGSLLVQINHPQVIRTLLLVADKNKPSLQRVAKYSKNFP
                     HATLAALAELLALKEPPARPGYPIIEDKKLPAQQKARDEYWRTLLQTLMASQPQLAAE
                     VMPWLSTQARAVLNSYLSAPPKPVIDSTDNSSLPEMLVSPPWRSKKKMTAPRLDLAPL
                     ELTPQIYWQPGEQERLAATESARYFSTESLAERMEQKSGRVVLQELGFGDDVWLFLNY
                     ILPGKLDAARNSLIVQWHYYQGRVEEILNGWNSPQAQLAEQALRSGHIEALINIWEND
                     NFSRYRPEKSVWNLYLLAQLPREMALTFWLRIIEKKHLFAGEDYFLSILGLDALPGLL
                     LAFSHRPKETFPLILNFGATELALPVARVWRRFAAQRDLARQWILQWPEHTASALIPL
                     VFTKPSDNSEAALLALRLLYEQGHGELLQTVANRWQRTDVWSALEQLLKQGPMDIYPA
                     RIPKAPDFWHPAMWSRPRLITNNQPVTGDALEIIGEMLRFTQGGRFYSGLEQLKTFCQ
                     PQTLAAFAWDLFTAWQQAGAPAKDNWAFLALSLFGDESTARDLTTQILAWPQEGKSAR
                     AVSGLNILTLMNNDMALIQLHHISQRAKSRPLRDNAAEFLQVVAENRGLSQEELADRL
                     VPTLGLDDPQALSFDFGPRQFTVRFDENLNPVIFNQQNVRQKSVPRLRADDDQLKAPE
                     ALARLKGLKKDATQVSKNLLPRLEAALRTTRRWSLADFHSLFVNHPFTRLVTQRLIWG
                     VYPANEPRCLLKAFRVAAEGEFCNAQDEPIDLPADALIGIAHPLEMTVEMRSEFAQLF
                     ADYEIMPPFRQLSRRTVLLTPDESTSNSLTRWEGKSATVGQLMGMRYKGWESGYEDAF
                     VYDLGEYRLVLKFSPGFNHYNVDSKALMSFRSLRVHRDNKSVTFAELDVFDLSEALSA
                     PDVIFH"
     gene            11522..>11758
                     /gene="yehK"
                     /locus_tag="ACHOSC_RS14865"
                     /old_locus_tag="pgaptmp_002973"
     CDS             11522..>11758
                     /gene="yehK"
                     /locus_tag="ACHOSC_RS14865"
                     /old_locus_tag="pgaptmp_002973"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YehK"
                     /protein_id="HGU4548874.1"
                     /translation="MIVQKELVAIYDYEIPVPENPFSFRLEIHKCSELFTGSVYRLER
                     FRLRPTFHQRDREDTDPLINDALIYIRDECIDERK"
BASE COUNT         3045 a         3007 c         2919 g         2787 t
ORIGIN      
        1 aagccgaaat tatttatata aatgatttcg gctttctaaa tgcaattaaa atcaggttgc
       61 caaattaatc gtagcgattt aacacgattc gacgtttgct atcgctacct gcaacaacca
      121 cttctgtcgt tacacctttg acgggcgaag aaaacagatg acgggtgtca acattatacg
      181 ttaactgacc acaatgctgt tgaggttgac cattaaccat agccatactg catgttgggg
      241 gactgatcct gccattaatc gctaatgtgg cagtctctga agagaatgcg ggagaagcca
      301 atccataggc aagaaaaata attcctgaca gccaatactt attcattgaa cgttatccct
      361 gtagtaaagg ttatgcctgg atagaatgag tgcataacaa actatagctg tacatccact
      421 acacagccac gaaggatgat aatgaagcat tgcctgtatg atcaatcgac tttgtagagt
      481 ttcggacgaa ggtccgcaga atattcgcag tattaaataa gtcttcatta atgcggcaaa
      541 aactaataca ccgcatcaat gtaacatctc tgcggtgtat gaagaataac cagaatggtt
      601 aataggcgtt agaccgcgcg gaaggaaatc tcgcctggaa tgacttcacc ctgccagtag
      661 agctgagctg caacgcggtc agccagctgg cggtagatag cggtaaattc gctctccgga
      721 cggctgataa ccgttggcgt gcctttatcg agatcctcac gtaaggagat gtgtaacggc
      781 atctgcccca gcagctgtgt gtggtatttt tcagccagtt tctcagcacc accggtaccg
      841 aaaatcggtt catggtgacc acagttactg caaatatgca cactcatgtt ctcgacaata
      901 ccaagcaccg gaacctcgac tttctcgaac atcacaatgc cttttttcgc atcgatcaac
      961 gcgatgtcct gcggggtggt gaccacgact gcaccagtta ctggaatgtt ttgcgccagc
     1021 gtcaattgaa tgtcaccggt gcctggcggc atatcaagca ccagatagtc gagatccggc
     1081 cacaaggttt cctgcaacat ctgcattaac gccttgctcg ccatcggtcc acgccacacc
     1141 atcgcattgt cgtcagtgac cagataacca attgaattgg ttgccaggcc atgagacata
     1201 atcggtgcca tgtgggtacc gtccggcgac gttggacgct gattttctgc gcccagcatg
     1261 gtgggaattg atgggccgta gatatcggcg tccagaatgc cgactttcgc cccttccgcc
     1321 gccagtgcca gcgccaggtt taccgccgtg gaggatttac ccacgccgcc tttaccggaa
     1381 ctgaccgcaa tgatgttctt cacaccatta atacccggct gattcttcac gcgttttaag
     1441 gtggcgatat tgtgcgacag tttccagtcg atagctttcg ccccggtgat acgcagcagt
     1501 tcggcgctac attgctcttt taactcttca aacgcgctat gccagacgaa tggcataacc
     1561 agttccacat gcaacgtgtc gtccatccag gcgacatggt gcaacgcttt cagcgtggtg
     1621 aggttatgct tcagggttgg gtgctgaaaa ttggccagtg tcccggcgac cattgcgcgc
     1681 aaggcttctg gtgatttggc ctgggattgt tcgttcatcc cgactccttt tttgtataga
     1741 taaaccatca gctgatagtt tacctgaaga atatagagaa gtacttactt aacattttcc
     1801 catttggtac tatctaaccc cttttcacta ttaagaagta atgcctacta tgactcaagt
     1861 cgcgaagaaa attctggtga cgtgcgcact gccgtacgct aacggctcaa tccacctcgg
     1921 ccatatgctg gagcacatcc aggctgatgt ctgggtccgt taccagcgaa tgcgcggcca
     1981 cgaggtcaac ttcatctgcg ccgacgatgc ccacggtaca ccgatcatgc tgaaagctca
     2041 gcagctcggt atcaccccgg agcagatgat tggcgaaatg agtcaggagc atcagactga
     2101 tttcgcaggc tttaacatca gctatgacaa ctatcactcg acgcacagcg aagagaaccg
     2161 ccagttgtca gaacttatct actctcgcct gaaagaaaac ggttttatta aaaaccgcac
     2221 catctctcag ctgtacgatc cggaaaaagg catgttcctg ccggaccgtt ttgtgaaagg
     2281 cacctgcccg aaatgtaaat ccccggatca atacggcgat aactgcgaag tctgcggcgc
     2341 gacctacagc ccgactgaat tgatcgagcc gaaatcggtg gtttctggcg ctacgccggt
     2401 aatgcgtgat tctgaacact tcttctttga tctgccctct ttcagcgaaa tgttgcaggc
     2461 atggacccgc agcggtgcgt tgcaggagca ggtggcaaat aaaatgcagg agtggtttga
     2521 atccggcctg caacagtggg atatctcccg cgacgcgcca tactttggtt ttgaaattcc
     2581 gaacgcgccg ggcaaatatt tctacgtctg gctggacgca ccgattggct acatgggttc
     2641 tttcaagaat ctgtgcgaca agcgcggcga cagcgtaagc ttcgatgaat actggaagaa
     2701 agactccacc gccgagctgt accacttcat cggtaaagat attgtttact tccacagcct
     2761 gttctggcct gccatgctgg aaggcagcaa cttccgcaag ccgaccaacc tgtttgttca
     2821 tggctatgtg acggtgaacg gcgcaaagat gtccaagtct cgcggcacct ttattaaagc
     2881 cagcacctgg ctgaatcatt ttgacgctga cagcctgcgt tactactaca ctgcgaaact
     2941 ctcttcgcgc attgatgata tcgatctcaa cctggaggat ttcgttcagc gtgtgaatgc
     3001 cgatatcgtg aacaaagtgg ttaacctggc ctctcgtaat gcgggcttta tcaacaagcg
     3061 ttttgacggc gtgctggcaa gcgaactggc tgacccgcag ttgtacaaaa ccttcactga
     3121 tgccgctgaa gtgattggtg aagcgtggga aagccgcgaa tttggtaaag cagtgcgcga
     3181 aatcatggcg ctggctgatc tggctaaccg ctatgtcgac gagcaggctc cgtgggtggt
     3241 ggcaaaacag gaaggccgcg atgccgacct gcaggcaatt tgctcaatgg gcataaacct
     3301 gttccgcgtg ctgatgactt acctgaagcc ggtactgccg aaactgaccg agcgcgcaga
     3361 agcattcctc aatacagaac tgacctggga tggtatccag caaccgctgc tgggccacaa
     3421 agtgaatccg ttcaaagcgc tgtataaccg catcgatatg aagcaggttg aagcactggt
     3481 ggaagcgtct aaagaagaag tgaaagcagc tgccgcgcca gttactggcc cactggcaga
     3541 tgatccgatt caggaaacca tcacctttga cgacttcgca aaagttgacc tgcgcgtggc
     3601 gctgatcgaa aacgcagagt ttgttgaagg ttctgacaaa ctgctgcgcc tgacactgga
     3661 tctcggcggt gaaaaacgca atgtcttctc cggtattcgt tctgcctacc cggatccgca
     3721 ggcactgatc gggcgtcaca ccattatggt ggccaacctg gcaccgcgta aaatgcgctt
     3781 tggtatctcc gaaggcatgg taatggctgc tggtcctggc gggaaagata ttttcctgct
     3841 aagcccggat gccggtgcta aaccgggtca tcaggtgaaa taatccccct tcaaggcgct
     3901 gcatcgacag cgccttttct ttataaattc ctaaagttgt tttcttgcga ttttgtctct
     3961 ctctaacccg cataaatact ggtagcatca gcattcaact ggataaaatt acagggatgc
     4021 agaatgaaac actttatcta tcaggacgaa aaatcacata aattctgggc agttgagcaa
     4081 cagggaaacg agttgcatat cagttgggga aaagttggca ccaaagggca aagccagata
     4141 aaaagttttt cagatgctgc ggcagcggca aaagcggagc ttaagctgat tgcggagaag
     4201 gtgaagaagg ggtatgtgga gcaagcgaag gataattctt tgcaaccttc ccaaacggta
     4261 acgggctctc tcaaggtagc ggacttatcc accattattc aggaacaacc ctctttcgta
     4321 gcagaaaccc gtgcgcctga caaaaataca gatgctgttt taccgtggct ggcgaaagat
     4381 attgctgtcg tttttccgcc cgaagttgta cacaccacgt taagtcatcg ccgctttccc
     4441 ggagttcctg ttcagcaagc agacaaattg acccaattac gtcgcttagc ctgtagtgtg
     4501 tcgcaacggg ataataaaac agccacattt gacttcagcg cctgttcttt agaatggcaa
     4561 aacaccgtcg cccaggcgat cagtcagatc gacggcctga aaacaacaca gctaccatca
     4621 ccagtaatgg ctgtactcac ggcacttgaa atgaaatgca caagatataa agtgcgtgag
     4681 gatgttatgg atcagatcgt ccaggaaggc ggtctggaat atgctactga tgtaataata
     4741 caccttcaac agattgatat taaatgggat tatgcgaata atgtcattat tattctgccg
     4801 tctggcattg cacctgacta cttggagcaa tattccagat ttgaattacg cctacgtaaa
     4861 catttatcac tggcggaaga gtctctctgg caaaaatgtg cacaaaaact tattgccgca
     4921 attccacata ttccagaatg acggcaacca ttaattgctt tgttattacc tgaaaaacca
     4981 gaaattgcac atgaaattgc ccagcgtcta ctggggcaaa aaaaattacc ctcgcttgag
     5041 tggttaaaaa tagtggcgac tgatgagcac attcttgcct cattagaaaa atatcacgag
     5101 ccatatgcca tttttgatga ttactattgt ggtgcgatat ggtcagccac cgtattacag
     5161 gagcaaggtg ttgcagccct gccccgattt gctccctatg ccgcaagtga ctactgcgcc
     5221 gatgtgttgc gtcatatcaa tcatccgttc gcattaacac tgcttatacg tgtagccggg
     5281 catactaaac gctgtcacga tcggatgacg aaagccattg ctgcgttccc acatgcagca
     5341 atggcggcac tgacggaact tcttgggcaa aaagaagaga acagttggcg cattatgcta
     5401 atgacaatgc ttatctcaca accagcactg gcagaacagg tcattccctg gctctcgaca
     5461 cccgcagttg ccgtactgaa atcatgccag caacaactga cacagccctc aaaccatgcc
     5521 agcgccgatc tactgccagc cgtagtagtc tcccctccct ggctttcgaa aaagaaaaaa
     5581 tcgccgattc cggtgctgga tttagcgcca ttaggcattg agccaatctg ttatctgaca
     5641 gaagaaatca gtaatcaact tttggcgaaa tatatctggt attcaaaaca catcacggtt
     5701 agccatgaag aaagtactac caacctgttg gcaaggatgg gttttcaacg acggatcgct
     5761 ggtacatata ttaaagctcc cgaagcggta gttgaggcat ggctaaatga agattattca
     5821 accttactaa gtgaatttaa ggtgtttcat tcacctaccg ggcattattg gcagttgggg
     5881 attttgacaa cattgccgct ggagaaagca gtaaaagcat ggaatgccct taccctatct
     5941 ccacataccg ataccgaata cgccatgtta cattttggac tcaaagggtt acctgggtta
     6001 gtaaactcac ttgcacgcta tccacaagaa gccttgccca tcacgaatta cttcgcagcg
     6061 agtgagctgg ctcctgccgt cgcccgtgcc ttcaacaaac tgaaaacgct acgcgaaaac
     6121 gcccgtagct ggctgttgaa atacccggaa catgccctta ccggcctgct gcctgcggcg
     6181 ctcggcaaag ccggtgaagc acaggataac gcccgcgctg ccttgcgtat gcttaccgaa
     6241 aacggtcatc agccattact gcaagaaatc gcccgacgtt ataaccagcc ggaagtaacc
     6301 gatgcggtga acgctctgct tgcgctcgat cccttagata atcacccgac aaaaatcccc
     6361 actcttccgg ccttttatca gccatcgctc tggacgcgcc cggtattaaa agcaaatgcc
     6421 caatcactgc cagatagcgc cctcctccac ctcggtgaaa tgctccgctt ccctcaggaa
     6481 gaggctctgt atccgggatt attgcaggtg aaagacgtct gttccgccga ctcactggcg
     6541 ggatttgcct gggatctgtt taccgcctgg cagaccgctg gcgcgccgtc gaaagagagt
     6601 tgggcgttca ctgcgttagg cgttctcggt aacgatgaca ccgcccgcaa actgacgcca
     6661 ttaatacgcg cctggcctgg tgaatcccag cataaacgcg ccaccgttgg gttggatatt
     6721 ctcgctgcta tcggtagtga tatcgccctt atgcagctta acggcatcgc ccagaaactg
     6781 aaattcaaag cattacagga gcgggcaaaa gaaaaaattg ccgacattgc cgagagccgc
     6841 gaactcacgg tggcggagct tgaagatcgg ttagcaccgg atctcggtct ggatgataac
     6901 ggttcgctgc tgctggattt tggcccacgg cagttcaccg tcagctttga tgaaacctta
     6961 aaaccgtttg tgcgtgatgt ttccggcagc cgcctgaaag acctgcccaa accgaacaaa
     7021 agcgatgatg aatcgcggtc gaacgatgcg gttaaccgct acaaattgct gaaaaaagat
     7081 gcgcgtaccg tcgccgccca gcaggtagca aggctggaat ccgccatgtg cctgcgccgc
     7141 cgctggtcgc cggaaaactt ccagctcttc ctggttgagc atccgctggt tcgccactta
     7201 acccgccgtc tgatttgggg cgtttatagc gccgaaaacc agctactggc ttgctttcgc
     7261 gtagcagaag ataacagcta cagcaccgct gacgatgatc ttttcaccct gccggaaggc
     7321 gatatctcta tcggcattcc tcacgttctg gagatatcac cgacggatgc cgccgccttt
     7381 gggcagcttt ttgccgacta tgaactgtta ccgccgttcc gccagctcga ccgtaacagt
     7441 tacgccctga cagaaaccga gcgcaatgcc agcgaattga cccgctgggc aggcagaaaa
     7501 tgcccgagcg gtcgggtcat gggactggcg aataaaggtt gggtgcgtgg tgaaccgcag
     7561 gatggtggct ggatcggctg gatgatcaaa cctctgggcc gctggtcgtt aatcatggaa
     7621 atcgatgaag gctttgcagt tggtatgtcg ccagccgaac ttagcgccga gcaactctta
     7681 agcaagctgt ggctatggga aggcaaagca gaaagctatg gctgggggag taattcaaca
     7741 caggaagcgc agttctccgt actcgatgcc atcaccgcca gcgagctaat taacgatatt
     7801 gaagccctgt ttgaataagg aaagagcatg gacaaggaat taccgtggct ggcggataac
     7861 gcccaactgg aactgaaata taaaaaaggc aaaacgccgc tcagtcatcg tcgctggccg
     7921 ggcgaaccag tgtccgttat cactggaagt ctcatccaga cattgggaga tgaattacta
     7981 caacaagcag gccagaagga aaatatcacc tggaattatg ataaatgctc actggagtgg
     8041 cagtccgcca tccagcaggc aatcaatttg actggcgagc ataaaccgtc aatcccagcc
     8101 cttaccatgg cggctctgat ctgtatcgcg caaaatgaca gccagcagtt acttgatgaa
     8161 atcgtgcaac aagaggggct ggaatatgcg acggatgtag tgatagcgcg tcagtgcatt
     8221 gcgcgacgtt atgagagtga ctcactggtc gtcactttgc agtatcagga cgaggactat
     8281 ggctacggtt atggctccgc gacctataac gatttcgatc tccgtttgcg taagcatctg
     8341 tcgctggcag aggaaagttg ctggcagcgt tgtgccgata aactcattgc cgcactgcca
     8401 ggaataccca aaattcgccg tccttttatt gcgctgatcc tcccggaaaa acctgaaata
     8461 gccaatgaac tcgccagtct tgaaagctct cggtcaagtc tccattcaaa ggagtggtta
     8521 aaggtcgttg ctactgataa tacggcggta aaaaaactcg aacgatactg gggcctggac
     8581 gtatttagcg atcgcgaagc cagctacatg tcacaagaaa accgcttcgg ctacgcggcc
     8641 tgcgcctccc ttttgcgcga acaaggactg gcagccgttc cgcgcctggc aatgtatgcc
     8701 cataaagaag attgcggcag tctgctggta caaattaacc atccgcaagt catccgcacc
     8761 ttgctactgg tggctgataa aaacaaaccc agcctgcaac gtgtggctaa atacagtaaa
     8821 aacttccccc acgcgacgct cgccgcactg gcagaactgc tggcgttaaa agaaccacca
     8881 gcccgcccag gttatccaat catcgaagac aaaaagctgc ctgcacagca aaaagcacgc
     8941 gatgaatact ggcgtacgct gttacaaacg ctgatggcat cgcagccaca actggcagca
     9001 gaagtgatgc cgtggttaag tactcaagcc agggcagtgc tgaatagtta tttatcggca
     9061 ccgccaaaac cggttattga tagcaccgat aacagcagtc tgcctgaaat gctcgtttca
     9121 ccaccgtggc gtagtaagaa aaaaatgaca gctccacgtc ttgatttggc accgctcgaa
     9181 ttaactccgc aaatttactg gcaaccaggc gaacaagaga ggcttgccgc cactgagtct
     9241 gcccgttatt tcagcacgga atctcttgcg gaacgcatgg aacaaaaaag tggacgagtt
     9301 gtattacagg aactgggttt tggggatgat gtatggctgt ttctgaatta tatactcccc
     9361 ggaaaactgg atgctgcacg caattcactc attgttcagt ggcattacta ccaggggcgg
     9421 gttgaagaga tcctgaatgg ctggaactcc ccgcaagcac aattagcaga acaggcgctc
     9481 cgcagcggtc acatagaagc gttaattaac atatgggaaa atgacaactt ctcacgttat
     9541 cgtccggaaa agagtgtctg gaacctgtat ttattggcac agttgccgcg tgagatggct
     9601 ttgaccttct ggctgcgtat cattgagaaa aagcatctgt tcgcgggtga ggactatttt
     9661 ctcagtatcc tcggattgga tgcgctacca ggtctgctgt tggctttttc acatcgtcca
     9721 aaagaaacat ttccgttaat tttaaatttt ggcgcaacag aactggcgct gcctgttgcc
     9781 cgcgtctggc gacgtttcgc ggcgcagcgt gatctggctc gccagtggat tttacaatgg
     9841 ccggaacata cggctagtgc acttatccct cttgtcttta ccaaacccag cgataatagc
     9901 gaagccgcat tacttgccct gcgtttactg tacgaacagg gacatggcga attgctacaa
     9961 accgtggcaa accgctggca gcgtacagat gtatggtctg ccctggagca gttgcttaaa
    10021 cagggtccaa tggacattta cccggcacgc attccaaaag cccctgattt ctggcatccg
    10081 gcaatgtggt ccaggcctcg acttatcact aataatcagc ctgttaccgg tgacgctctg
    10141 gaaattatcg gcgaaatgct gcgctttacc caggggggac gtttttatag cgggctggaa
    10201 caactgaaaa cgttctgcca gccacaaacg ctggcagctt ttgcctggga tctcttcact
    10261 gcgtggcaac aagctggtgc ccccgcaaaa gacaactggg catttctggc gttaagtctc
    10321 tttggtgacg aaagcacggc acgggatctg acgacacaga tcctcgcctg gccacaagaa
    10381 ggcaaatctg cccgtgctgt cagcggcctg aacatcctta ccctgatgaa taatgatatg
    10441 gcgctgatac agctgcatca tatatcgcaa cgggctaaat cccgcccctt acgtgataac
    10501 gcggcggaat ttcttcaggt agtcgcagaa aatcgcgggc taagccagga agagctagcg
    10561 gacagattag tcccaaccct gggccttgat gatccgcagg cgttgagttt tgattttggt
    10621 ccccggcagt ttaccgttcg cttcgatgaa aacctcaacc cggttatctt taatcagcaa
    10681 aacgttcgcc agaaaagcgt tccccggttg cgcgccgatg acgatcaact gaaagcgccc
    10741 gaggcactgg cccgactaaa agggctaaaa aaagatgcta ctcaggtgag caaaaacctg
    10801 ctcccgcgtc ttgaagctgc cctacgtacc acccgacgct ggtcgctggc agattttcat
    10861 tctctgtttg ttaatcatcc ctttacccgt ctggttaccc agcgattaat atggggggtt
    10921 tatccggcaa atgaaccgcg ttgtttactc aaagcctttc gtgtggccgc agagggggag
    10981 ttctgcaatg cgcaagatga gccaattgac ctgcctgcgg acgctctgat tggcattgcc
    11041 cacccgttag aaatgacagt agaaatgcgc agtgaatttg cacagctttt tgccgattac
    11101 gaaattatgc cgccttttcg ccagttgtcg cgccgcacgg tgctgctcac acctgacgag
    11161 tcaaccagta acagcctgac tcgctgggaa ggtaaatccg ctaccgttgg gcagcttatg
    11221 ggaatgcgat acaaaggctg ggagtcaggt tatgaggacg catttgtcta tgacctgggt
    11281 gagtaccggc tggtccttaa gttttcaccc ggttttaacc actacaatgt tgatagcaaa
    11341 gcgctaatga gcttccgttc tcttcgagtg caccgtgaca ataaatccgt cacttttgcc
    11401 gaacttgatg tgtttgattt gagtgaggcg ttaagcgcac ctgacgtcat tttccattaa
    11461 aaacacagcg ggcagtgatg caactgcccg ttatcaacac gacttaccag cggggaaagc
    11521 gatgatcgtg caaaaagagc tggttgctat ttacgattat gagatccctg tacctgaaaa
    11581 tccgttttcc ttcagacttg agatccataa atgctctgaa ttatttacag gttccgtcta
    11641 tcgactggag cgattccggc tacgtccaac atttcatcaa cgtgatcgag aagatactga
    11701 cccgctaata aatgatgcgt tgatttatat aagagatgag tgtattgatg agcggaaa
//
LOCUS       DAZKHF020000134        11729 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2201S31241
            SAMN43267800-rid25106413.denovo.135, whole genome shotgun sequence.
ACCESSION   DAZKHF020000134 DAZKHF020000000
VERSION     DAZKHF020000134.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267800
            Sequence Read Archive: SRR30307263
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 11729)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11729)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 11729)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350581.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350581.2
            Genome Coverage       :: 151x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:21:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,652
            CDSs (total)                      :: 5,557
            Genes (coding)                    :: 5,320
            CDSs (with protein)               :: 5,320
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 237
            CDSs (without protein)            :: 237
            Pseudo Genes (ambiguous residues) :: 0 of 237
            Pseudo Genes (frameshifted)       :: 102 of 237
            Pseudo Genes (incomplete)         :: 130 of 237
            Pseudo Genes (internal stop)      :: 52 of 237
            Pseudo Genes (multiple problems)  :: 41 of 237
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11729
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267800-rid25106413.denovo.135"
                     /serotype="O 26:H11"
                     /isolate="2201S31241"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-01"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            299..1597
                     /gene="kgtP"
                     /locus_tag="ACHOSC_RS14870"
                     /old_locus_tag="pgaptmp_002974"
     CDS             299..1597
                     /gene="kgtP"
                     /locus_tag="ACHOSC_RS14870"
                     /old_locus_tag="pgaptmp_002974"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-ketoglutarate permease"
                     /protein_id="HGU4548875.1"
                     /translation="MAESTVTADSKLTSSDTRRRIWAIVGASSGNLVEWFDFYVYSFC
                     SLYFAHIFFPSGNTTTQLLQTAGVFAAGFLMRPIGGWLFGRIADKHGRKKSMLLSVCM
                     MCFGSLVIACLPGYETIGTWAPALLLLARLFQGLSVGGEYGTSATYMSEVAVEGRKGF
                     YASFQYVTLIGGQLLALLVVVVLQHTMEDAALREWGWRIPFALGAVLAVVALWLRRQL
                     DETSQQETRALKEAGSLKGLWRNRRAFIMVLGFTAAGSLCFYTFTTYMQKYLVNTAGM
                     HANVASGIMTAALFVFMLIQPLIGALSDKIGRRTSMLCFGSLAAIFTVPILSALQNVS
                     SPYAAFGLVMCALLIVSFYTSISGILKAEMFPAQVRALGVGLSYAVANAIFGGSAEYV
                     ALSLKSIGMETAFFWYVTLMAVVAFLVSLMLHRKGKGMRL"
     gene            complement(1594..1917)
                     /gene="yfiM"
                     /locus_tag="ACHOSC_RS14875"
                     /old_locus_tag="pgaptmp_002975"
     CDS             complement(1594..1917)
                     /gene="yfiM"
                     /locus_tag="ACHOSC_RS14875"
                     /old_locus_tag="pgaptmp_002975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417081.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfiM family lipoprotein"
                     /protein_id="HGU4548876.1"
                     /translation="MRILFVCSLLLLSGCSHMANDSWSGQDKAQHFIASAMLSAAGNE
                     YSQHQGMSRDRSAMFGLMFSVSLGASKELWDSRPEGSGWSWKDLAWDVAGASTGYTVW
                     QLTRH"
     gene            complement(1963..3318)
                     /gene="pssA"
                     /locus_tag="ACHOSC_RS14880"
                     /old_locus_tag="pgaptmp_002976"
     CDS             complement(1963..3318)
                     /gene="pssA"
                     /locus_tag="ACHOSC_RS14880"
                     /old_locus_tag="pgaptmp_002976"
                     /EC_number="2.7.8.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008444 -
                     CDP-diacylglycerol-glycerol-3-phosphate
                     3-phosphatidyltransferase activity [Evidence IEA];
                     GO_process: GO:0032049 - cardiolipin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol--serine
                     O-phosphatidyltransferase"
                     /protein_id="HGU4548877.1"
                     /translation="MLSKFKRNKHQQHLAQLPKISQSVDDVDFFYAPADFRETLLEKI
                     ASAKQRICIVALYLEQDDGGKGILNALYEAKRQRPELDVRVLVDWHRAQRGRIGAAAS
                     NTNADWYCRMAQENPGVDVPVYGVPINTREALGVLHFKGFIIDDSVLYSGASLNDVYL
                     HQHDKYRYDRYHLIRNRKMSDIMFEWVTQNIMNGRGVNRLDDVNRPKSPEIKNDIRLF
                     RQELRDAAYHFQGDADNDQLSVTPLVGLGKSSLLNKTIFHLMPCAEQKLTICTPYFNL
                     PAILVRNIIQLLREGKKVEIIVGDKTANDFYIPEDEPFKIIGALPYLYEINLRRFLSR
                     LQYYVNTDQLVVRLWKDDDNTYHLKGMWVDDKWMLITGNNLNPRAWRLDLENAILIHD
                     PQLELAPQREKELELIREHTTIVKHYRDLQSIADYPVKVRKLIRRLRRIRIDRLISRI
                     L"
     gene            complement(3432..6092)
                     /gene="pat"
                     /locus_tag="ACHOSC_RS14885"
                     /old_locus_tag="pgaptmp_002977"
     CDS             complement(3432..6092)
                     /gene="pat"
                     /locus_tag="ACHOSC_RS14885"
                     /old_locus_tag="pgaptmp_002977"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein lysine acetyltransferase"
                     /protein_id="HGU4548878.1"
                     /translation="MSQRGLEALLRPKSIAVIGASMKPNRAGYLMMRNLLAGGFNGPV
                     LPVTPAWKAVLGVLAWPDIASLPFTPDLAVLCTNASRNLALLEELGEKGCKTCIILSA
                     PASQHEDLRACALRHNMRLLGPNSLGLLAPWQGLNASFSPVPIKRGKLAFISQSAAVS
                     NTILDWAQQRKMGFSYFIALGDSLDIDVDELLDYLARDSKTSAILLYLEQLSDARRFV
                     SAARSASRNKPILVIKSGRSPAAQRLLNTTAGMDPAWDAAIQRAGLLRVQDTHELFSA
                     VETLSHMRPLRGDRLMIISNGAAPAALALDALWSRNGKLATLSEETCQKLRDALPGHV
                     AISNPLDLRDDASSEHYIKTLDILLHSQDFDALMVIHSPSAAAPATESAQVLIEAVKH
                     HPRSKYVSLLTNWCGEHSSQEARRLFSEAGLPTYRTPEGTITAFMHMVEYRRNQKQLR
                     ETPALPSNLTSNTAEAHLLLQQAIAEGATSLDTHEVQPILQAYGMNTLPTWIASDSTE
                     AVHIAEQIGYPVALKLRSPDIPHKSEVQGVMLYLRTANEVQQAANAIFDRVKMAWPQA
                     RVHGLLVQSMANRAGAQELRVVVEHDPVFGPLIMLGEGGVEWRPEDQAVVALPPLNMN
                     LARYLVIQGIKSKKIRARSALRPLDVAGLSQLLVQVSNLIVDCPEIQRLDIHPLLASG
                     SEFTALDVTLDISPFEGDNESRLAVRPYPHQLEEWVELKNGERCLFRPILPEDEPQLQ
                     QFISRVTKEDLYYRYFSEINEFTHEDLANMTQIDYDREMAFVAVRRIDQTEEILGVTR
                     AISDPDNIDAEFAVLVRSDLKGLGLGRRLMEKLITYTRDHGLQRLNGITMPNNRGMVA
                     LARKLGFNVDIQLEEGIVGLTLNLAQREES"
     gene            complement(6124..6822)
                     /gene="tapT"
                     /locus_tag="ACHOSC_RS14890"
                     /old_locus_tag="pgaptmp_002978"
                     /gene_synonym="tuaA"
                     /gene_synonym="yfiP"
     CDS             complement(6124..6822)
                     /gene="tapT"
                     /locus_tag="ACHOSC_RS14890"
                     /old_locus_tag="pgaptmp_002978"
                     /gene_synonym="tuaA"
                     /gene_synonym="yfiP"
                     /EC_number="2.5.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417078.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA-uridine aminocarboxypropyltransferase"
                     /protein_id="HGU4548879.1"
                     /translation="MTENGVLQLRAERIARATRPFLARGNRVRRCQRCLLPEKLCLCS
                     TITPAQAKSRFCLLMFDTEPMKPSNTGRLIADILPDTVAFQWSRTEPSQDLLELVQNP
                     DYQPMVVFPASYADEQREVIFTPPAGKPPLFIMLDGTWPEARKMFRKSPYLDNLPVIS
                     VDLSRLSAYRLREAQAAGQYCTAEVAIALLDMASDTGAAAGLGEHFTRFKTRYLAGKT
                     QHLGSITAEQLESV"
     gene            complement(6891..7310)
                     /gene="trxC"
                     /locus_tag="ACHOSC_RS14895"
                     /old_locus_tag="pgaptmp_002979"
     CDS             complement(6891..7310)
                     /gene="trxC"
                     /locus_tag="ACHOSC_RS14895"
                     /old_locus_tag="pgaptmp_002979"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015035 - protein-disulfide reductase
                     activity [Evidence IEA];
                     GO_process: GO:0006662 - glycerol ether metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin TrxC"
                     /protein_id="HGU4548880.1"
                     /translation="MNTVCTHCQAINRIPDDRIEDAAKCGRCGHDLFDGEVINATGET
                     LDKLLKDDLPVVIDFWAPWCGPCRNFAPIFEDVAQERSGKVRFVKVNTEAERELSSRF
                     GIRSIPTIMIFKNGQVVDMLNGAVPKAPFDSWLNESL"
     gene            7517..8554
                     /gene="yfiF"
                     /locus_tag="ACHOSC_RS14900"
                     /old_locus_tag="pgaptmp_002980"
     CDS             7517..8554
                     /gene="yfiF"
                     /locus_tag="ACHOSC_RS14900"
                     /old_locus_tag="pgaptmp_002980"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA/rRNA methyltransferase"
                     /protein_id="HGU4548881.1"
                     /translation="MNDEMKGKSGKVKVMYVRSDDDSDKRTHNPRTGKGGGRPGKSRA
                     DGGRRPARDDKQSQPRDRKWEDSPWRTVSRAPGDETPEKADHGGISGKSFIDPEVLRR
                     QRAEETRVYGENACQALFQSRPEAIVRAWFIQSVTPRFKEALRWMAANRKAYHVVDEA
                     ELTKASGTEHHGGVCFLIKKRNGTTVQQWVSQAGAQDCVLALENESNPHNLGGMMRSC
                     AHFGVKGVVVQDAALLESGAAIRTAEGGAEHVQPITGDNIVNVLDDFRQAGYTVVTTS
                     SEQGKPLFKTSLPAKMVLVLGQEYEGLPDAARDPNDLRVKIDGTGNVAGLNISVATGV
                     LLGEWWRQNKA"
     gene            complement(8602..9291)
                     /gene="ung"
                     /locus_tag="ACHOSC_RS14905"
                     /old_locus_tag="pgaptmp_002981"
     CDS             complement(8602..9291)
                     /gene="ung"
                     /locus_tag="ACHOSC_RS14905"
                     /old_locus_tag="pgaptmp_002981"
                     /EC_number="3.2.2.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004844 - uracil DNA N-glycosylase
                     activity [Evidence IEA];
                     GO_function: GO:0016799 - hydrolase activity, hydrolyzing
                     N-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HGU4548882.1"
                     /translation="MANELTWHDVLAEEKQQPYFLNTLQTVASERQSGVTIYPPQKDV
                     FNAFRFTELGDVKVVILGQDPYHGPGQAHGLAFSVRPGIAIPPSLLNMYKELENTIPG
                     FTRPNHGYLESWARQGVLLLNTVLTVRAGQAHSHASLGWETFTDKVISLINQHREGVV
                     FLLWGSHAQKKGAIIDKQRHHVLKAPHPSPLSAHRGFFGCNHFVLANQWLEQRGETPI
                     DWMPVLPAESE"
     gene            9596..9979
                     /gene="grcA"
                     /locus_tag="ACHOSC_RS14910"
                     /old_locus_tag="pgaptmp_002982"
     CDS             9596..9979
                     /gene="grcA"
                     /locus_tag="ACHOSC_RS14910"
                     /old_locus_tag="pgaptmp_002982"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007728348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="autonomous glycyl radical cofactor GrcA"
                     /protein_id="HGU4548883.1"
                     /translation="MITGIQITKAANDDLLNSFWLLDSEKGEARCIVAKAGYAEDEVV
                     AVSKLGDIEYREVPVEVKPEVRVEGGQHLNVNVLRRETLEDAVKHPEKYPQLTIRVSG
                     YAVRFNSLTPEQQRDVIARTFTESL"
     gene            complement(10035..10622)
                     /gene="eamB"
                     /locus_tag="ACHOSC_RS14915"
                     /old_locus_tag="pgaptmp_002983"
     CDS             complement(10035..10622)
                     /gene="eamB"
                     /locus_tag="ACHOSC_RS14915"
                     /old_locus_tag="pgaptmp_002983"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine/O-acetylserine transporter"
                     /protein_id="HGU4548884.1"
                     /translation="MTPTLLSAFWTYTLITAMTPGPNNILALSSATSHGFRQSTRVLA
                     GMSLGFLIVMLLCAGISFSLAVIDPAAVHLLSWAGAAYIVWLAWKIATSPTKEDGLQA
                     KPISFWASFALQFVNVKIILYGVTALSTFVLPQTQALSWVVGVSVLLAMIGTFGNVCW
                     ALAGHLFQRLFRQYGRQLNIVLALLLVYCAVRIFY"
     gene            10725..11606
                     /gene="yfiE"
                     /locus_tag="ACHOSC_RS14920"
                     /old_locus_tag="pgaptmp_002984"
     CDS             10725..11606
                     /gene="yfiE"
                     /locus_tag="ACHOSC_RS14920"
                     /old_locus_tag="pgaptmp_002984"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417072.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HGU4548885.1"
                     /translation="MDLRRFITLKTVVEEGSFLRASQKLCCTQSTVTFHIQQLEQEFS
                     VQLFEKIGRRMCLTREGKKLLPHIYELTRVMDTLREAAKKESDPDGELRVVSGETLLS
                     YRMPQVLQRFRQRAPKVRLSLQSLNCYVIRDALLNDEADVGVFYRVGNDDALNRRELG
                     EQSLVLVASPQIADVDFTEPGRHNASSFIINEPQCVFRQIFESTLRQRRITVENTIEL
                     LSIESIKRCVAANIGVSYLPRFAVEKELESGELIELPFGEQSQTITAMCAHHAGKAVS
                     PAMHTFIQCVEESFVAG"
BASE COUNT         2917 a         2928 c         3034 g         2850 t
ORIGIN      
        1 gtcaggatgg cctttttgcg ttggtgaaaa aaatgcctga tgcgacgctg cgcgtcttat
       61 actcccacat atgccagatt cagcagcgaa tacggcttcc ccaacttgcc cacctccata
      121 cgtgttctcc tcaccagaaa tttatcctta agctcctcaa taaccatttt cctgctaact
      181 aaattcatgg ttaaggttgc ataatgatac gcaacaaatg tataacattt tctttaccaa
      241 aagaaataaa caaaagcgac cgacaaaagc atcggattac ggcaggagac ataatggcat
      301 ggctgaaagt actgtaacgg cagacagcaa actgacaagt agtgatacac gtcgccgcat
      361 ttgggcgatt gtgggggcct cttcaggtaa tctggtcgag tggttcgatt tctatgtcta
      421 ctcgttctgt tcactctact tcgcccacat tttcttccct tccgggaaca cgacgactca
      481 actactacaa acagcaggtg tttttgctgc gggattcctg atgcgcccaa taggcggttg
      541 gctatttggc cgcatagccg ataaacatgg tcgcaaaaaa tcgatgctgt tatcggtgtg
      601 tatgatgtgt ttcggatcgc tggttatcgc ctgcctccca ggttatgaaa ctataggtac
      661 gtgggctccg gcattattgc ttctcgctcg tttatttcag ggattatccg ttggcggaga
      721 atatggcacc agcgccacct atatgagtga agttgccgtt gaagggcgca aaggttttta
      781 cgcatcattt cagtatgtga cgttgatcgg cggacaactg ctagccctac tggttgtcgt
      841 ggttttacaa cacaccatgg aagacgctgc actcagagag tggggatggc gtattccttt
      901 cgcgttagga gctgtgttag ctgttgtggc gttgtggtta cgtcgtcaat tagatgaaac
      961 ttcgcaacaa gaaacgcgcg ctttaaaaga agctggatct ctgaaaggat tatggcgcaa
     1021 tcgccgtgca ttcatcatgg ttctcggttt taccgctgcg ggctcccttt gtttctatac
     1081 cttcaccact tatatgcaga agtatctggt aaatactgcg ggaatgcatg ccaacgtggc
     1141 gagtggcatt atgactgccg cattgtttgt attcatgctt attcaaccac tcattggcgc
     1201 gctgtcggat aagattggtc gccgtacctc aatgttatgt ttcggttcgc tggcagccat
     1261 ttttaccgtt cctattctct cagcattgca aaacgtttcc tcgccttatg ccgcttttgg
     1321 tctggtgatg tgtgccctgc tgatagtgag tttttataca tcaatcagtg gaatactgaa
     1381 ggctgagatg ttcccggcac aggttcgcgc attaggcgtt ggcctgtcat atgcggtcgc
     1441 taatgctata tttggtggtt cggcggagta cgtagcgttg tcgctgaaat caataggaat
     1501 ggaaacagcc ttcttctggt atgtgacctt gatggccgtg gtggcgtttc tggtttcttt
     1561 gatgctacat cgcaaaggga aggggatgcg tctttagtga cgggtcagtt gccagacggt
     1621 atagccggtg cttgcaccgg cgacatccca ggccaaatcc ttccagctcc agccgctccc
     1681 ttcggggcgg ctatcccaaa gctcttttga cgcccccaaa ctgacagaga acatcaatcc
     1741 aaacatggca ctgcgatccc ggctcatccc ctgatgctgt gaatattcat ttccggcggc
     1801 ggaaagcatc gccgaggcga taaagtgttg agctttatcc tgcccgctcc agctatcgtt
     1861 agccatatgg ctgcatccag aaagtaacaa tagcgaacag acaaaaagaa tacgcaaaac
     1921 gtggcctcca aaaaacaaac cccgtacagg acggggttgt gattacagga tgcggctaat
     1981 taatcggtcg atgcggatac ggcgcaaacg gcggatgagt ttacgaacct tcaccggata
     2041 atcggcaata ctttgcagat cgcgatagtg cttaacgatg gtggtatgct cgcggatcag
     2101 ctccagttct ttctctcgct gcggcgcaag ctccagttgt ggatcgtgga tcaaaatggc
     2161 gttttccaga tccagacgcc aggcgcgcgg gttcaggtta ttaccggtga tcaacatcca
     2221 cttatcatca acccacatcc cttttaggtg ataggtgttg tcgtcatctt tccataaccg
     2281 aaccactagc tggtcagtat tgacgtaata ctgcaaacgg ctcaggaaac gacgcagatt
     2341 gatctcatag agataaggca atgcgccaat tatcttgaaa ggttcatctt ccggaatgta
     2401 gaagtcgttc gcggttttat caccaacaat aatttcgacc tttttccctt cgcgcagcaa
     2461 ctggataata ttgcgcacaa ggattgctgg caggttgaag tatggcgtac agatggttag
     2521 tttctgctcg gcacaaggca taagatggaa aatggtcttg ttcaacagac tcgatttccc
     2581 cagccccact agcggcgtta cggaaagctg atcgttgtcg gcatcgccct ggaaatgata
     2641 agcggcatca cgcagctcct ggcggaacag acgaatatcg ttcttgattt ccgggctttt
     2701 tggccgatta acatcatcca gacgattaac gccgcggcca ttcataatat tctgtgtaac
     2761 ccattcaaac ataatgtctg acatcttacg gttacggata agatgataac ggtcgtagcg
     2821 atatttatcg tgctgatgca ggtaaacatc gttcaggctg gcaccgctat aaagtacgct
     2881 atcgtcgatg ataaagcctt taaagtgcag aacaccaagg gcttcacggg tattgattgg
     2941 aacgccataa accggaacat ctacgcccgg attttcctgc gccatgcggc agtaccagtc
     3001 agcgttagtg ttagatgccg cagcgccaat gcgtccacgt tgtgcacgat gccagtcgac
     3061 cagcacccgc acatccagtt ccggacgctg ccttttagct tcatacaacg cgttcagaat
     3121 gcctttgcca ccgtcatcct gttcgagata cagggcgaca atgcaaatgc gctgcttcgc
     3181 gctggctatt ttttccagca gcgtctcccg gaagtcggcg ggagcgtaaa agaaatcgac
     3241 atcatcaact gattgagaaa tcttgggtag ttgggcaagg tgttgttgat gtttattacg
     3301 cttaaatttt gacaacatca cagtgcattt cttctctgtt cattgaaggg tcctctgtgc
     3361 aatgcagacg acataagcgg gcaataataa caccagtccc gattaagtgg tcaacatttc
     3421 cagtacctta ctcatgattc ctcgcgttgg gcaagattta gcgtaagccc aacgatcccc
     3481 tcttcgagct ggatatcaac gttaaacccg agcttgcggg ctagcgccac catgccacga
     3541 ttgtttggca tcgtaatacc attcagacgt tgtagtccgt gatctcgcgt ataggtaatc
     3601 aacttttcca ttaagcgtcg acctaagcct aaccctttga gatccgagcg aaccagcaca
     3661 gcaaattcgg catcgatgtt atcaggatcg gaaatcgcac gcgtgacgcc gaggatctct
     3721 tccgtttgat caatacgtcg taccgctaca aacgccattt cccgatcgta gtcgatctgt
     3781 gtcatgttgg ctaaatcttc atgggtaaat tcgttgatct cgctaaagta gcggtaataa
     3841 agatcttctt tggtgactcg cgaaatgaat tgctgaagtt gtggctcatc ttctggcaaa
     3901 atcgggcgga acaagcagcg ttcaccgttt ttcaattcta cccattcttc cagctgatgc
     3961 ggataagggc gcactgccag ccgactctcg ttatcgcctt caaacggcga gatatccagc
     4021 gtgacatcca gcgcggtaaa ttcactgcca gaagccagca aaggatgaat atccagacgc
     4081 tgaatttccg ggcaatcgac aatcaagttg gaaacctgca ccagaagctg gctcaagcct
     4141 gcaacatcca atgggcgtag cgcactgcgt gcacgaatct ttttactttt gatcccctga
     4201 ataaccagat agcgggccag gttcatgttc agcggcggta acgcgacgac ggcttgatct
     4261 tcaggacgcc actccacacc gccttcgccc agcatgatca acggcccgaa aaccggatcg
     4321 tgctcaacca caacccgcaa ctcctgagca ccagcacggt tagccatact ttgcaccaac
     4381 aggccgtgga cccgtgcctg cggccaggcc atttttacgc gatcgaaaat ggcgtttgcc
     4441 gcttgctgga cttcattggc tgtacgcagg tagagcatga cgccctgaac ttccgattta
     4501 tgtggaatat ccggcgaacg caatttcagc gccaccggat aaccaatctg ttcggcaata
     4561 tgcaccgctt cggtgctatc gctggcaatc caggtgggga gcgtgttcat gccatatgct
     4621 tgcaggatag gctgaacttc atgggtatcg agtgacgttg ctccttcggc aatcgcctgt
     4681 tgcaacagaa gatgcgcttc tgcggtattg gaggtcagat tgctgggcaa cgccggcgtt
     4741 tcgcgtagtt gcttctgatt acgccggtac tccaccatat gcataaaagc agtgatggtt
     4801 ccttccgggg tacggtaggt cggcagcccg gcttcgctga ataatcgtcg tgcctcttgc
     4861 gaggaatgtt cgccacacca gttcgtcagc agagaaacat acttgctgcg gggatgatgc
     4921 tttaccgctt caattaatac ttgcgcgctt tctgttgcgg gagcagcggc gctgggcgaa
     4981 tgaataacca tcagcgcatc aaaatcctgg ctgtggagca gaatgtccag cgttttaata
     5041 tagtgctcac tgctggcgtc atcgcgcaga tcgagcgggt tagagattgc cacatgtcct
     5101 ggcagtgcat cgcgcagttt ctggcaggtt tcttcgctta gtgttgccag cttgccattg
     5161 cgtgaccata aggcatcaag cgccagcgcg gcaggcgcag caccgttgct gataatcatc
     5221 aaccggtcgc cacgcagcgg gcgcatatgg ctaagggttt ccaccgccga aaacagttcg
     5281 tgggtgtcct gtacccgcaa caaaccggca cgctgaatag ccgcatccca cgccggatcc
     5341 attcctgccg tggtgttcaa cagacgttga gccgccgggc tacgtccgct tttaatcacc
     5401 agaatcggtt tgttgcgaga ggcactacgg gccgccgaaa caaagcgtcg cgcgtcgctt
     5461 aactgttcga gatagagcag gatggcgctg gttttactgt cgcgtgccag atagtcaagc
     5521 aattcatcaa cgtcgatatc caggctgtcg ccgagcgcaa taaagtagga aaagcccatc
     5581 ttacgctgtt gcgcccagtc gaggatggtg ttggagacgg cagccgattg cgaaataaac
     5641 gccagcttgc cgcgtttaat cggcacaggc gaaaagctgg cattcagacc ttgccaggga
     5701 gccagtaaac ccagactgtt tggtccaagc aggcgcatgt tatggcgcag ggcgcaggcg
     5761 cggagatctt cgtgttgcga tgccggggcg gaaagaataa tgcaggtttt acagcctttc
     5821 tcgccgagct cttccagaag agcaagatta cggctggcat tggtacataa aaccgcaagg
     5881 tcgggtgtaa agggcaagct ggcaatatcc ggccaggcca acacacccaa caccgctttc
     5941 caggctggcg tcaccgggag taccggtccg ttaaagcctc ccgccagcag gttacgcatc
     6001 atcaggtaac ctgcgcgatt gggtttcatc gacgcgccaa ttaccgctat cgattttggt
     6061 cgcagtagtg cttccagtcc tcgctgactc ataccggtct cccgcagaag tgaccgaatg
     6121 attttaaacg ctttctaact gttctgctgt gatgctaccc agatgttgcg tttttcctgc
     6181 cagatagcgt gttttaaagc gggtaaaatg ctcgcctaac cctgctgccg ccccggtatc
     6241 gctggccata tctaacagtg cgatggctac ctcggcagta caatattggc ctgcagcctg
     6301 ggcttcacgc aggcgatagg cagaaagccg ggaaagatcg acggaaatga cgggaagatt
     6361 atccagatac ggacttttac gaaacatctt gcgagcttcc ggccaggtac catcgagcat
     6421 gataaacagc ggtggcttac cggcaggtgg tgtgaagatc acttcccgtt gctcatcagc
     6481 atacgaagcg ggaaagacca ccattggctg atagtccggg ttttgcacca actccagcaa
     6541 atcctgcgag ggttcggtac gcgaccattg aaacgcaacg gtatcaggca aaatatcagc
     6601 aatcagacgc ccggtattac tgggcttcat tggctcggtg tcgaacatca gcaaacagaa
     6661 gcgacttttt gcttgtgctg gggtaattgt cgaacagaga cataatttct ctggcaaaag
     6721 acagcgttgg cagcgacgaa cgcgattacc gcgggcaaga aaaggacgtg ttgcgcgcgc
     6781 aatacgctcg gcgcgtaact ggagaacacc gttttcggtc ataagagagc gtcgaaaaaa
     6841 cgccattgtc gcagaggaga aaacggggca caagatgcgc cccggtaaga ttaaagagat
     6901 tcgttcagcc agctatcgaa cggcgctttc ggtactgcgc cattaagcat gtcgacaacc
     6961 tgaccgtttt tgaaaatcat gatcgtcggt atactacgaa ttccaaagcg actgctcaat
     7021 tcacgttcag cttcggtatt cactttcaca aagcgcactt taccgctacg ctcttgcgcg
     7081 acatcttcaa aaattggtgc gaaattacgg caggggccgc accacggtgc ccagaagtcg
     7141 atcaccacag gtagatcatc cttcagcaat ttgtcgagtg tttcaccggt cgcattaatc
     7201 acctctccgt caaacaagtc gtgaccgcag cgtccgcatt ttgccgcatc ttcgatccga
     7261 tcgtcgggaa tgcgattgat ggcctgacaa tgggtacaaa cggtattcat aactaacctc
     7321 gggatgagta aggagactta tcggcaacga cgccggtatg tttctaatat gttacatatt
     7381 atcggggaca ttgttccaga caacaatctg ttttattcaa taggtatgat agtcgcaggc
     7441 tttggtacga aatcatggcg aaccgcgcgg acttcgggta atctgcgcgc ttcgcgcagc
     7501 gctggtggag aaaagcatga acgatgaaat gaaaggtaaa agcggcaagg tcaaagtgat
     7561 gtatgtccgc agtgatgatg attctgataa acgtacccac aacccgcgta ccgggaaagg
     7621 gggcgggcgt ccaggaaaat ctcgtgctga cggtggccgt cgccccgccc gcgatgacaa
     7681 acagagtcag ccccgtgacc gcaagtggga agattcgccg tggcgcacgg tttcccgcgc
     7741 gccgggtgat gagacgccgg aaaaggccga tcacggtggc atcagtggta aaagttttat
     7801 tgatccggaa gtgttgcgtc gtcagcgtgc ggaagaaacc cgcgtctacg gcgaaaatgc
     7861 atgtcaggca cttttccaga gccgtccgga agcgattgtt cgcgcctggt ttatccagag
     7921 tgtaacgccg cgttttaaag aagccttgcg ctggatggca gcaaaccgca aagcgtacca
     7981 tgtggtggat gaagcggaat tgacaaaagc gtcaggcacg gaacatcacg gcggcgtttg
     8041 cttcttgatc aaaaagcgta acggtacaac cgtgcagcag tgggtaagcc aggcaggcgc
     8101 gcaggattgc gttctggcac tggaaaacga atctaacccg cataacctgg gtggcatgat
     8161 gcgcagctgc gcgcacttcg gcgtgaaagg tgtcgtggtg caggatgcgg cactgctgga
     8221 gtcgggggcg gctatccgta ccgcagaagg cggcgcagag cacgttcagc cgattactgg
     8281 cgacaacatt gttaacgtgc tggatgattt ccgtcaggca ggttacaccg tagtgaccac
     8341 ttccagcgag cagggtaaac cgctgttcaa aactagtctg ccagcgaaaa tggtactggt
     8401 gctgggtcag gaatatgaag ggttaccgga tgccgcacgc gatccgaacg atctgcgcgt
     8461 gaagattgac ggtacgggca acgttgccgg gctgaatatc tctgtcgcaa ccggcgtttt
     8521 gcttggtgaa tggtggcgtc agaataaagc ctgataaatc agccgggtgg caactctgcc
     8581 atccggcatt tccccgcaaa tttactcact ctctgccggt aatactggca tccagtcaat
     8641 cggcgtctcg ccacgttgtt ccagccactg atttgccagc acaaaatggt tgcagccaaa
     8701 gaatccacga tgcgccgaaa gcggcgacgg atgcggtgct ttcagtacat gatggcgttg
     8761 cttatctata atcgcccctt tcttttgcgc atgtgatccc cacaacaaaa acaccacgcc
     8821 ttcgcggtgc tggttaatca ggctgatgac tttatcagta aacgtctccc aaccgaggct
     8881 ggcgtgggaa tgcgcctgac ctgcgcgaac cgtcaacaca gtattgagca gcagaacgcc
     8941 ctgacgcgcc cagctttcga gatagccatg attagggcgg gtgaagcccg gaatagtatt
     9001 ttccagctct ttatacatat tcaataatga cggaggaatg gcaatgccgg gacgaacgga
     9061 aaatgccaga ccatgcgcct gtcccggtcc gtgataagga tcctggccga gaatcaccac
     9121 tttaacgtca cccaactcgg taaagcggaa cgcgttaaag acatcttttt gtggtggata
     9181 gatagtgacg ccggactgcc gctcgctggc gacggtctga agggtattaa gaaaataagg
     9241 ttgttgcttc tcttcagcca gcacgtcatg ccaggttaat tcgttagcca tctcgctctc
     9301 ctgcgaatct tcaatccgcc tagcttaact gcttctttct acagaacaaa atcatgctca
     9361 ttttttagac ttaaacgagt gcaaaaaaat gtaaattggt aaaatttcac acccgcataa
     9421 gttgatgtaa aacaataaaa tccatttacg accacaatcg atgtgtggtt tttattgatt
     9481 taaatcaaag aatcaagggt ggttgaggag tatatataca ctcaagcaac aatggtttta
     9541 ccaattggcc gcgacaggct gaacaaatca aataattttg ccggggaggc atcacatgat
     9601 tacaggtatc cagattacta aagccgctaa cgacgatctg ctgaactctt tctggctgct
     9661 ggacagcgaa aaaggcgaag cacgttgcat cgttgcaaaa gcaggttatg cagaagatga
     9721 agtggttgca gtaagcaaac tgggtgacat tgaataccgt gaagttccag tagaagtgaa
     9781 accagaagtt cgcgttgaag gtggtcaaca cctgaacgtt aacgttctgc gtcgtgaaac
     9841 tctggaagat gcagttaagc atccggaaaa atatccgcag ctgaccatcc gtgtatccgg
     9901 ttatgcagtt cgctttaact ctctgactcc ggaacagcag cgcgacgtta tcgctcgtac
     9961 ctttactgaa agcctgtaag gcttaagtag caagttacta agcggaagag ggcgacctct
    10021 tccgcttttt ttcgttaata gaaaatgcgt accgcgcaat agaccagcaa cagggcaagc
    10081 acgatattta actggcgacc atactggcga aacaatcgct gaaacagatg ccccgccagc
    10141 gcccagcaca cattgccaaa cgtcccaatc atcgccagca aaacgctgac gccaactacc
    10201 cagcttagcg cctgtgtttg cggcagaaca aacgtcgaca gtgccgtaac accgtacaaa
    10261 atgattttga cgttcacaaa ctgcaaagca aagctggccc aaaagctgat tggttttgcc
    10321 tgaagtccgt cttcctttgt tgggctggtg gcgattttcc acgccagcca gacaatatat
    10381 gccgcccccg cccaactcaa aaggtgtacc gctgccgggt caatcactgc cagtgaaaat
    10441 gaaatgcccg cacacagtaa catcacaatc aaaaatccca gactcatccc tgccagcacg
    10501 cgggtacttt gacgaaatcc atgcgacgta gcagagctaa gggcgagaat attgttcggt
    10561 cctggcgtca tagcggtaat cagggtgtaa gtccaaaaag cacttaaaag ggtcggtgtc
    10621 acttgttatc tcctctgttt tggtagacag tacccgacac ggaacattta aaaaaatgaa
    10681 tgtttttata ttctagattc gatattttcg ataggtttgg ggttatggat ctgcgccgtt
    10741 ttattacgct taaaaccgtg gtggaagagg gttccttttt gcgagcttcg caaaaattgt
    10801 gctgtacaca atcgacggtg acttttcata ttcagcagct tgagcaggag ttttcagtcc
    10861 agttatttga gaaaattggc cgacggatgt gccttacccg cgaaggaaaa aagttgctgc
    10921 cgcacattta tgaacttacc cgagtgatgg atacgcttcg tgaggctgcc aaaaaggagt
    10981 cagatccgga cggggaacta cgcgttgttt cgggcgaaac gttgctctct taccgaatgc
    11041 cgcaggtgtt gcagcgtttt cggcaacgcg caccaaaagt gcgtttatcg ttgcagtcgc
    11101 tgaactgtta cgtgatccgt gatgcgctac tgaatgatga ggccgatgtc ggcgtctttt
    11161 atcgcgtagg gaatgatgat gccctgaacc gacgagagtt gggtgaacaa tcgctggtgc
    11221 tggtggcttc accgcaaatt gcagatgtcg attttactga gccgggaaga cataacgcca
    11281 gtagctttat tatcaacgaa ccgcaatgtg tcttccggca gatatttgag agcacgctgc
    11341 gtcagcggcg gatcacggtg gaaaacacca ttgagctgtt aagcattgaa agtatcaaac
    11401 gttgtgtcgc ggcgaatatc ggtgtcagtt acttgccccg ctttgcggtg gaaaaagagc
    11461 ttgagagcgg agaattaatt gaattgcctt ttggcgaaca gtcgcagacc attacggcca
    11521 tgtgcgcaca ccatgctgga aaagcggtta gcccggcgat gcacactttt attcagtgtg
    11581 ttgaagagag ttttgtggct ggataaaaat aatgtcggga ttaaacccgg catttaactt
    11641 gtcgacaaag tgcgctttat tcatgccgga tgcggcgtga acgccttatc cggcctacaa
    11701 gatcgtgcaa attcaatata ttgcatgag
//
LOCUS       DAZKHF020000135        11696 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2201S31241
            SAMN43267800-rid25106413.denovo.136, whole genome shotgun sequence.
ACCESSION   DAZKHF020000135 DAZKHF020000000
VERSION     DAZKHF020000135.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267800
            Sequence Read Archive: SRR30307263
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 11696)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11696)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 11696)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350581.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350581.2
            Genome Coverage       :: 151x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:21:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,652
            CDSs (total)                      :: 5,557
            Genes (coding)                    :: 5,320
            CDSs (with protein)               :: 5,320
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 237
            CDSs (without protein)            :: 237
            Pseudo Genes (ambiguous residues) :: 0 of 237
            Pseudo Genes (frameshifted)       :: 102 of 237
            Pseudo Genes (incomplete)         :: 130 of 237
            Pseudo Genes (internal stop)      :: 52 of 237
            Pseudo Genes (multiple problems)  :: 41 of 237
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11696
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267800-rid25106413.denovo.136"
                     /serotype="O 26:H11"
                     /isolate="2201S31241"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-01"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            complement(56..352)
                     /gene="yaaX"
                     /locus_tag="ACHOSC_RS14925"
                     /old_locus_tag="pgaptmp_002985"
     CDS             complement(56..352)
                     /gene="yaaX"
                     /locus_tag="ACHOSC_RS14925"
                     /old_locus_tag="pgaptmp_002985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2502 domain-containing protein"
                     /protein_id="HGU4548886.1"
                     /translation="MKKMQSIVLALSLVLVAPMAAQAAEITLVPSVKLQIGDRDNRGY
                     YWDGGHWRDHGWWKQHYEWRGNRWHPHGPPPPPRHHKKAHHDHHGGHGPGKHHR"
     gene            complement(566..1852)
                     /gene="thrC"
                     /locus_tag="ACHOSC_RS14930"
                     /old_locus_tag="pgaptmp_002986"
     CDS             complement(566..1852)
                     /gene="thrC"
                     /locus_tag="ACHOSC_RS14930"
                     /old_locus_tag="pgaptmp_002986"
                     /EC_number="4.2.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004795 - threonine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009088 - threonine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine synthase"
                     /protein_id="HGU4548887.1"
                     /translation="MKLYNLKDHNEQVSFAQAVTQGLGKNQGLFFPHDLPEFSLTEID
                     EMLKLDFVTRSAKILSAFIGDEIPQEILEERVRAAFAFPAPVANVESDVGCLELFHGP
                     TLAFKDFGGRFMAQMLTHIAGDKPVTILTATSGDTGAAVAHAFYGLPNVKVVILYPRG
                     KISPLQEKLFCTLGGNIETVAIDGDFDACQALVKQAFDDEELKVALGLNSANSINISR
                     LLAQICYYFEAVAQLPQEARNQLVVSVPSGNFGDLTAGLLAKSLGLPVKRFIAATNVN
                     DTVPRFLHDGQWSPKATQATLSNAMDVSQPNNWPRVEELFRRKIWQLKELGYAAVDDE
                     TTQQTMRELKELGYTSEPHAAVAYRALRDQLNPGEYGLFLGTAHPAKFKESVEAILGE
                     TLDLPKELAERADLPLLSHNLPADFAALRKLMMNHQ"
     gene            complement(1853..2785)
                     /gene="thrB"
                     /locus_tag="ACHOSC_RS14935"
                     /old_locus_tag="pgaptmp_002987"
     CDS             complement(1853..2785)
                     /gene="thrB"
                     /locus_tag="ACHOSC_RS14935"
                     /old_locus_tag="pgaptmp_002987"
                     /EC_number="2.7.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004413 - homoserine kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0009088 - threonine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine kinase"
                     /protein_id="HGU4548888.1"
                     /translation="MVKVYAPASSANMSVGFDVLGAAVTPVDGALLGDVVTVEAAEIF
                     SLNNLGRFADKLPSEPRENIVYQCWERFCQELGKQIPVAMTLEKNMPIGSGLGSSACS
                     VVAALMAMNEHCGKPLNDTRLLALMGELEGRISGSIHYDNVAPCFLGGMQLMIEENDI
                     ISQQVPGFDEWLWVLAYPGIKVSTAEARAILPAQYRRQDCIAHGRHLAGFIHACYSRQ
                     PELAAKLMKDVIAEPYRERLLPGFRQARQAVAEIGAVASGISGSGPTLFALCDKPDTA
                     QRVADWLGKNYLQNQEGFVHICRLDTAGARVLEN"
     gene            complement(2787..5249)
                     /gene="thrA"
                     /locus_tag="ACHOSC_RS14940"
                     /old_locus_tag="pgaptmp_002988"
     CDS             complement(2787..5249)
                     /gene="thrA"
                     /locus_tag="ACHOSC_RS14940"
                     /old_locus_tag="pgaptmp_002988"
                     /EC_number="1.1.1.3"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004072 - aspartate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0004412 - homoserine dehydrogenase
                     activity [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0008652 - amino acid biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009067 - aspartate family amino acid
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional aspartate kinase/homoserine
                     dehydrogenase I"
                     /protein_id="HGU4548889.1"
                     /translation="MRVLKFGGTSVANAERFLRVADILESNARQGQVATVLSAPAKIT
                     NHLVAMIEKTISGQDALPNISDAERIFAELLTGLAAAQPGFPLAQLKTFVDQEFAQIK
                     HVLHGISLLGQCPDSINAALICRGEKMSIAIMAGVLEARGHNVTVIDPVEKLLAVGHY
                     LESTVDIAESTRRIAASRIPADHMVLMAGFTAGNEKGELVVLGRNGSDYSAAVLAACL
                     RADCCEIWTDVDGVYTCDPRQVPDARLLKSMSYQEAMELSYFGAKVLHPRTITPIAQF
                     QIPCLIKNTGNPQAPGTLIGASRDEDELPVKGISNLNNMAMFSVSGPGMKGMVGMAAR
                     VFAAMSRARISVVLITQSSSEYSISFCVPQSDCVRAERAMQEEFYLELKEGLLEPLAV
                     TERLAIISVVGDGMRTLRGISAKFFAALARANINIVAIAQGSSERSISVVVNNDDATT
                     GVRVTHQMLFNTDQVIEVFVIGVGGVGGALLEQLKRQQSWLKNKHIDLRVCGVANSKA
                     LLTNVHGLNLENWQEELAQAKEPFNLGRLIRLVKEYHLLNPVIVDCTSSQAVADQYAD
                     FLREGFHVVTPNKKANTSSMDYYHLLRHAAEKSRRKFLYDTNVGAGLPVIENLQNLLN
                     AGDELMKFSGILSGSLSYIFGKLDEGMSFSEATTLAREMGYTEPDPRDDLSGMDVARK
                     LLILARETGRELELADIEIEPVLPAEFNAEGDVAAFMANLSQLDDRFAARVAKARDEG
                     KVLRYVGNIDEDGVCRVKIAEVDGNDPLFKVKNGENALAFYSHYYQPLPLVLRGYGAG
                     NDVTAAGVFADLLRTLSWKLGV"
     misc_feature    complement(5276..5395)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00506"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Thr leader region; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /db_xref="RFAM:RF00506"
     gene            complement(5331..5396)
                     /gene="thrL"
                     /locus_tag="ACHOSC_RS14945"
                     /old_locus_tag="pgaptmp_002989"
     CDS             complement(5331..5396)
                     /gene="thrL"
                     /locus_tag="ACHOSC_RS14945"
                     /old_locus_tag="pgaptmp_002989"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thr operon leader peptide"
                     /protein_id="HGU4548890.1"
                     /translation="MKRISTTIITTITITTGNGAG"
     gene            complement(5610..6296)
                     /gene="yjtD"
                     /locus_tag="ACHOSC_RS14950"
                     /old_locus_tag="pgaptmp_002990"
     CDS             complement(5610..6296)
                     /gene="yjtD"
                     /locus_tag="ACHOSC_RS14950"
                     /old_locus_tag="pgaptmp_002990"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710140.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA/rRNA methyltransferase"
                     /protein_id="HGU4548891.1"
                     /translation="MRITIILVAPARAENIGAAARAMKTMGFSELRIVDSQAHLEPAT
                     RWVAHGSGDIIDNIKVFPTLVESLHDVDFTVATTARSRAKYHYYATPVELVPLLEEKS
                     SWMSHAALVFGREDSGLTNEELALADVLTGVPMVADYPSLNLGQAVMVYCYQLATLIQ
                     QPTKSDTTADQHQLQALRERVMALLTTLAVADDIKLVDWLQQRLGLLEQRDTAMLHRL
                     LHDIEKNITK"
     gene            complement(6696..6836)
                     /gene="yjjY"
                     /locus_tag="ACHOSC_RS14955"
                     /old_locus_tag="pgaptmp_002991"
     CDS             complement(6696..6836)
                     /gene="yjjY"
                     /locus_tag="ACHOSC_RS14955"
                     /old_locus_tag="pgaptmp_002991"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YjjY"
                     /protein_id="HGU4548892.1"
                     /translation="MTKVRNCVLDALSINVNNIISLVVGTFPQDPTVSKTAVILTILT
                     AT"
     gene            6932..7648
                     /gene="arcA"
                     /locus_tag="ACHOSC_RS14960"
                     /old_locus_tag="pgaptmp_002992"
     CDS             6932..7648
                     /gene="arcA"
                     /locus_tag="ACHOSC_RS14960"
                     /old_locus_tag="pgaptmp_002992"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003621071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator ArcA"
                     /protein_id="HGU4548893.1"
                     /translation="MQTPHILIVEDELVTRNTLKSIFEAEGYDVFEATDGAEMHQILS
                     EYDINLVIMDINLPGKNGLLLARELREQANVALMFLTGRDNEVDKILGLEIGADDYIT
                     KPFNPRELTIRARNLLSRTMNLGTVSEERRSVESYKFNGWELDINSRSLIGPDGEQYK
                     LPRSEFRAMLHFCENPGKIQSRAELLKKMTGRELKPHDRTVDVTIRRIRKHFESTPDT
                     PEIIATIHGEGYRFCGDLED"
     gene            complement(7708..9060)
                     /gene="creD"
                     /locus_tag="ACHOSC_RS14965"
                     /old_locus_tag="pgaptmp_002993"
     CDS             complement(7708..9060)
                     /gene="creD"
                     /locus_tag="ACHOSC_RS14965"
                     /old_locus_tag="pgaptmp_002993"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell envelope integrity protein CreD"
                     /protein_id="HGU4548894.1"
                     /translation="MLKSPLFWKMTTLFGAVLLLLIPIMLIRQVIVERADYRSDVEDA
                     IRQSTSGPQKLVGPLIAIPVTELYTVQEEDKTVERKRSFIHFWLPESLMVDGNQNVEE
                     RKIGIYTGQVWHSDLTLKADFDVSRLSELNAPNIILGKPFIVISVGDARGIGVVKAPE
                     VNGTALTIEPGTGLEQGGQGVHIPLPEGDWRKQNLKLNMALNLSGTGDLSVVPAGRNS
                     EMTLTSNWPHPSFLGDFLPAKREVSESGFLAQWQSSWFANNLGERFASGNDTGWENFP
                     AFSVAVTTPADQYQLTDRATKYAILLIALTFMAFFVFETLTAQRLHPMQYLLVGLSLV
                     MFYLLLLALSEHIGFTVAWIIASLIGALMNGIYLQAVLKGWRNSMLFTLALLLLDGVM
                     WGLLNSADSALLLGTSVLVVALAGMMFVTRNIDWYAFSLPKMKASKEVTMDDQLRIWK
                     "
     gene            complement(9118..10542)
                     /gene="creC"
                     /locus_tag="ACHOSC_RS14970"
                     /old_locus_tag="pgaptmp_002994"
     CDS             complement(9118..10542)
                     /gene="creC"
                     /locus_tag="ACHOSC_RS14970"
                     /old_locus_tag="pgaptmp_002994"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     CreC"
                     /protein_id="HGU4548895.1"
                     /translation="MRIGMRLLLGYFLLVAVAAWFVLAIFVKEVKPGVRRATEGTLID
                     TATLLAELARPDLLSGDPTHGQLAQAFNQLQHRPFRANIGGINKVRNEYHVYMTDSQG
                     KVLFDSANKAVGQDYSRWNDVWLTLRGQYGARSTLQNPADPESSVMYVAAPIMDGSRL
                     IGVLSVGKPNAAMAPVIKRSERRILWASAILLGIALVIGAGMVWWINRSIARLTRYAD
                     SVTDNKPVPLPELGSSELRKLAQALESMRVKLEGKNYIEQYVYALTHELKSPLAAIRG
                     AAEILREGPPPEVVARFTDNILTQNARMQALVETLLRQARLENRQEVVLTVVDVAALF
                     RRVSEARTVQLAEKNITLHVMPTEVNVAAEPALLEQALGNLLDNAIDFTPESGRITLS
                     AEVDQEHVALKVLDTGSGIPDYALSRIFERFYSLPRANGQKSSGLGLAFVSEVARLFN
                     GEVTLRNVQEGGVLASLRLHRHFT"
     gene            complement(10542..11231)
                     /gene="creB"
                     /locus_tag="ACHOSC_RS14975"
                     /old_locus_tag="pgaptmp_002995"
     CDS             complement(10542..11231)
                     /gene="creB"
                     /locus_tag="ACHOSC_RS14975"
                     /old_locus_tag="pgaptmp_002995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator CreB"
                     /protein_id="HGU4548896.1"
                     /translation="MQRETVWLVEDEQGIADTLVYMLQQEGFAVEVFERGLPVLDKAR
                     QQAPDVMILDVGLPDISGFELCRQLLALHPALPVLFLTARSEEVDRLLGLEIGADDYV
                     AKPFSPREVCARVRTLLRRVKKFSSPSPVIRIGHFELNEPAAQISWFDTPLTLTRYEF
                     LLLKTLLKSPGRVWSRQQLMDSVWEDAQDTYDRTIDTHIKTLRAKLRAINPDLSPINT
                     HRGMGYSLRGL"
     gene            complement(11244..>11696)
                     /gene="creA"
                     /locus_tag="ACHOSC_RS14980"
                     /old_locus_tag="pgaptmp_002996"
     CDS             complement(11244..>11696)
                     /gene="creA"
                     /locus_tag="ACHOSC_RS14980"
                     /old_locus_tag="pgaptmp_002996"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein CreA"
                     /protein_id="HGU4548897.1"
                     /translation="LSLSLIMLGPLAHAEEIGSVDTVFKMIGPDHKIVVEAFDDPDVK
                     NVTCYVSRAKTGGIKGGLGLAEDTSDAAISCQQVGPIELSDRIKNGKAQGEVVFKKRT
                     SLVFKSLQVVRFYDAKRNALAYLAYSDKVVEGSPKNAISAVPVMPWRQ"
BASE COUNT         2893 a         3220 c         2795 g         2788 t
ORIGIN      
        1 cgcgccagcg tcgcatcagg cgctgaatgc cggattgtta cccggcattt gtcatttagc
       61 ggtgatgttt gcctggacca tgaccgccgt gatgatcatg atgagctttc ttatggtgac
      121 gcggcggtgg cggcggtccg tgtgggtgcc agcgattgcc tcgccattca taatgttgtt
      181 tccaccagcc gtggtcgcgc cagtgaccgc catcccagta ataaccgcga ttatcacgat
      241 cgcctatctg taattttact gacgggacta acgtaatttc cgcagcctgc gctgccatgg
      301 gagcgaccag aaccagggaa agtgcgagta cgatagattg catctttttc acaggttact
      361 ccttttatca ttgcccgtta tgggtcgata tccggagatt acgcgatgag aacggcggtt
      421 gttattctcc gcaatcctaa tctctaagtt accgcattta ttgagaattt ctcctttttc
      481 cctgtcgttt ctctccatat tttcttcaca aaaaagccgg gctgcataaa agcaaacccg
      541 gcctgattga gataatgaat agatgttact gatgattcat catcaattta cgcaacgcag
      601 caaaatcggc gggcagatta tgtgaaagca agggtaaatc agcacgttct gccagctctt
      661 ttggcagatc caacgtttca ccgagaatcg cttccacgct ctctttaaat ttcgccggat
      721 gcgcggtgcc gaggaacaag ccatattcgc ctggattcaa ctggtcacgc agcgcacgat
      781 acgctacggc agcgtgcggc tccgaggtgt agcccagttc ttttaactca cgcattgtct
      841 gttgcgtggt ttcatcatcc acggctgcat aacccagctc tttcagttgc cagattttgc
      901 ggcggaacaa ctcttccaca cgcggccagt tgttcggctg actcacgtcc atcgcgttgg
      961 ataacgtcgc ctgagtcgct ttgggggacc actgaccgtc gtgcaggaaa cgtggcacgg
     1021 tatcgttcac gttggtcgca gcaataaaac gtttcaccgg cagaccgagt gacttcgcca
     1081 gcagacccgc cgtcaaatcg ccgaagtttc cgcttggtac cgaaacaacc agctggttgc
     1141 gcgcctcctg cggcagctgc gcaacagctt caaagtagta gcaaatctgc gccagcaaac
     1201 ggctgatgtt aatcgagtta gctgagttta accccagcgc cactttcagt tcttcatcat
     1261 caaacgcctg cttcaccagc gcctgacagg catcgaaatc gccgtcgatg gcaacagttt
     1321 cgatattgcc gcccaatgta cagaacagtt tttcttgcag tggactgatt ttgcctcgtg
     1381 gatagaggat aaccactttc acattcggca aaccgtagaa agcatgagcc actgccgctc
     1441 cggtatcacc ggaggtcgcg gtcagaatgg tcactggctt atcacccgca atatgggtca
     1501 gcatttgtgc cataaagcga ccgccgaaat ctttaaatgc cagcgttggc ccgtggaaca
     1561 attccagaca accgacatcg ctttcaacat tggcgaccgg agccgggaag gcaaacgccg
     1621 cgcgcacgcg ctcttccagg atttcctgcg ggatttcatc accaataaac gccgagagga
     1681 tcttcgcact gcgggtgaca aaatccagct tcagcatctc atcaatttca gtcaggctga
     1741 attccggcag gtcatgcgga aaaaacagcc cctgattttt gcccaacccc tgggttacgg
     1801 cttgcgcaaa gctgacctgc tcgttgtgat ctttcagatt gtagagtttc atttagtttt
     1861 ccagtactcg tgcgcccgcc gtatccagcc ggcaaatatg aacaaaacct tcctgatttt
     1921 gcaggtagtt cttacccaac cagtcggcaa cgcgctgggc ggtatccggc ttgtcacaca
     1981 gagcgaacaa ggtcgggccg gagccggaga taccgctcgc taccgcgccg atttccgcga
     2041 ccgcctgccg cgcctgccgg aagcctggca gtaatcgttc acggtagggt tcagcgataa
     2101 catctttcat cagcttcgcg gcaagctcag gctgacggga atagcaggcg tgaatgaagc
     2161 ctgccagatg tcgcccgtgc gcaatgcaat cctggcggcg atactgcgcc ggtaaaatag
     2221 cccgggcttc tgccgtcgag actttaatcc ccggatacgc cagcacccac agccactcat
     2281 caaacccagg cacttgctgg ctgatgatgt cgttttcttc gatcatcaac tgcataccac
     2341 ctagaaaaca cggtgccacg ttgtcgtaat gaatgctgcc ggagatacgt ccttccagct
     2401 cgcccatcaa agccagcaaa cgagtgtcat taagcggctt gccgcagtgt tcattcatcg
     2461 ccatcagcgc cgcgaccacc gaacaggcgc tggagcctaa gcccgaaccg atcggcatat
     2521 tcttttccag agtcatcgcc actggaattt gcttgccaag ctcctggcaa aaacgctccc
     2581 agcactgata aacgatattt tcccgtggtt ctgacggcag cttatcggca aagcgcccga
     2641 ggttgttgag actgaatatc tctgccgcct caaccgtaac tacatctccg agcaatgcac
     2701 catcaacggg tgtcaccgcc gccccgagca catcaaaccc gacgctcata ttggcactgg
     2761 aagccggggc ataaacttta accatgtcag actcctaact tccatgagag ggtacgcagc
     2821 agatcggcaa agacaccagc agcggtaacg tcattgcccg caccatatcc gcgcagcacc
     2881 aacggcagcg gctgataata gtggctatag aaggccaggg cgttttcgcc atttttcact
     2941 ttgaacagcg gatcattacc atccacttcg gcaatcttca cgcgacagac gccatcttca
     3001 tcaatattgc caacatagcg caaaactttt ccttcatcac gggccttcgc cacgcgcgcg
     3061 gcaaagcgat cgtcgagctg tgacagattc gccataaaag cggcaacatc accctcggcg
     3121 ttaaactctg cgggcagcac aggttcaatt tcaatatccg ccagctccag ttcacgtccc
     3181 gtttcacggg cgagaatcaa tagtttacgc gccacatcca taccagaaag atcatctcgc
     3241 ggatccggtt cggtataacc catttcccgt gccagcgtgg tcgcctcgga gaaactcatg
     3301 ccttcgtcta acttgccgaa gatataagaa agcgatcctg aaagaatgcc ggagaacttc
     3361 atcaattcat caccagcatt gagcagattt tgcaggtttt caataaccgg taatccagcc
     3421 ccaacgttgg tgtcatagag gaatttccgc cgcgattttt ccgccgcatg acgcaacaga
     3481 tggtagtaat ccatcgacga ggtgttggcc tttttgttcg gcgtgacaac gtggaaacct
     3541 tcgcgcagga agtcggcata ttgatccgcc actgcctggc tggaggtgca gtcaacaatg
     3601 accgggttca gcagatgata ttctttcacg aggcgaatta agcgcccgag attaaacggc
     3661 tctttggctt gcgccagttc ttcctgccag ttttccagat ttaggccatg cacattggtg
     3721 agcagtgcct tcgagttggc aacaccgcag acacgtaagt cgatatgttt attcttcagc
     3781 cagctttgtt gacgcttcag ttgctccagc agcgcaccgc caacgccacc gacgccaatc
     3841 acaaacactt cgataacctg atcggtattg aacagcatct gatgagtaac gcgcacgcca
     3901 gtggtcgcat catcgttatt taccacgaca gagattgagc gttcagaaga tccctgagca
     3961 atagcgacaa tgttgatatt ggcgcgggcc agcgcggcaa agaatttcgc cgagatccca
     4021 cgcaaggtac gcataccatc acctaccacc gagataatgg ccagccgttc cgtcaccgcc
     4081 agcggctcca gtaagccttc tttcagttcc aggtagaact cttcctgcat tgcccgttca
     4141 gcccgcacac agtcgctttg cggaacgcag aaactgatac tgtattcgga agatgattgc
     4201 gtaatcagca ccacggaaat acgggcgcgt gacatcgcgg caaagacgcg cgccgccatg
     4261 ccgaccattc ctttcatccc cgggccggaa acgctgaaca ttgccatgtt attcagattg
     4321 gaaatgccct tgaccggtaa ttcgtcttca tcacgactgg caccaatgag cgtacctggt
     4381 gcttgaggat ttccggtatt tttaatcagg caagggatct ggaactgggc gatgggggta
     4441 atggtgcggg ggtgaagaac tttagcgccg aagtaggaaa gctccatcgc ttcctggtag
     4501 gacatcgact tcaacaacct cgcatcgggc acctgacgcg ggtcgcaggt atagaccccg
     4561 tcaacgtccg tccaaatctc gcaacaatcg gcgcgtaaac aggcagccag caccgccgcg
     4621 gagtagtcgg aaccgttgcg tccaagtacc accagttcgc ctttttcatt accggcggtg
     4681 aagcctgcca tcagcaccat gtgatcagcc ggaatacgac ttgccgcaat acggcgggtg
     4741 gactcagcaa tatcgacggt agattcgagg taatgcccca ctgccagtag tttttcgacc
     4801 ggatcgataa cggtaacgtt gtgaccacgc gcttctaaca cgccggccat aatggcgatc
     4861 gacattttct cgccacggca aatcagcgca gcgttaatgc tatccgggca ctgccctaac
     4921 aaactaatgc catgcaggac atgttttatt tgggcaaatt cctggtcgac gaaagttttc
     4981 aattgcgcca gcgggaatcc cggctgggcg gcggcgagtc ccgtcaaaag ttcggcaaaa
     5041 atacgttcgg catcgctgat attgggtaaa gcatcctggc cgctaatggt tttttcaatc
     5101 atcgccacca ggtggttggt gattttggcg ggggcagaga ggacggtggc cacctgcccc
     5161 tgcctggcat tgctttccag aatatcggca acccgcagaa aacgttctgc atttgccact
     5221 gatgtaccgc cgaacttcaa cactcgcatg gttgttacct cgttaccttt ggtcgaaaaa
     5281 aaaagcccgc actgtcaggt gcgggctttt ttctgtgttt cctgtacgcg tcagcccgca
     5341 ccgttacctg tggtaatggt gatggtggtg ataatggtgg tgctaatgcg tttcatggat
     5401 gttgtgtact ctgtaatttt tatctgtctg tgcgctatgc ctatattggt taaagtattt
     5461 agtgacctaa gtcaataaaa ttttaattta ctcacggcag gtaaccagtt cagaagctgc
     5521 tgtcagagac tcttttttta atccacacag agacatattg cccattgcag tcagaatgaa
     5581 aagctgaaaa atacttacta aggcgttttt tatttggtga tatttttttc aatatcatgc
     5641 agcaaacggt gcaacattgc cgtgtctcgt tgctctaaaa gccccaggcg ttgctgtaac
     5701 cagtcgacca gttttatgtc atctgccact gccagagtcg tcagcaatgc catgactcgt
     5761 tcgcgtaaag cttgcagttg atgttggtct gccgttgtgt cacttttcgt cggttgttgt
     5821 attaatgttg ctaattgata gcaatagacc atcactgcct gccccagatt gagcgaagga
     5881 taatccgcca ccatcggcac accagtaaga acgtcagcca acgctaactc ttcgttagtc
     5941 aacccggaat cttcgcgacc aaacaccagc gcggcatggc tcatccatga agatttttcc
     6001 tctaacagcg gcaccagttc aactggcgtg gcgtagtaat gatatttcgc ccgactgcgc
     6061 gcagtggtgg cgacagtgaa atcgacatcg tgtaacgatt caaccagtgt cgggaaaact
     6121 ttaatattat caataatatc accagatcca tgtgcgaccc agcgagtggc tggctccaga
     6181 tgtgcctgac tatcgacaat ccgcagttcg ctaaacccca tcgttttcat tgcccgcgcc
     6241 gctgccccaa tattttctgc tctggcgggt gcgaccagaa taatcgttat acgcatattg
     6301 ccactcttct tgaccaaatg accgcgaaca gggtgatcac tgtcaactta ttacgcggtg
     6361 cgaatttaca aatccttaac gtaagtcgca gaaaaagccc tttacttagc ttaaaaaagg
     6421 ctaaactatt tcctgactgt actaacggtt gaattgttaa aaaatgctac atatccttct
     6481 gtttacttag gataatttta taaaaaataa atctcgacaa ttggattcac cacgtttatt
     6541 agttgtatga tgcaactagt tggattatta aaataatgtg acgaaagcta gcatttagat
     6601 acgatgattt catcaaactg ttaacgtgct acaattgaac ttgatatatg tcaacgaagc
     6661 gtagttttat tgggtgtccg gcccctctta gcctgttatg ttgctgttaa aatggttagg
     6721 atgacagccg tttttgacac tgtcgggtcc tgagggaaag tacccacgac caagctaatg
     6781 atgttgttga cgttgatgga aagtgcatca agaacgcaat tacgtacttt agtcatgtta
     6841 cgccgatcat gttaatttgc agcatgcatc aggcaggtca gggacttttg tacttcctgt
     6901 ttcgatttag ttggcaattt aggtagcaaa catgcagacc ccgcacattc ttatcgttga
     6961 agacgagttg gtaacacgca acacgttgaa aagtattttc gaagcggaag gctatgatgt
     7021 tttcgaagcg acagatggcg cggaaatgca tcagatcctc tctgaatatg acatcaacct
     7081 ggtgatcatg gatatcaatc tgccgggtaa gaacggtctt ctgttagcgc gtgaactgcg
     7141 cgagcaggcg aatgttgcgt tgatgttcct gactggccgt gacaacgaag tcgataaaat
     7201 tctcggcctc gaaatcggtg cagatgacta catcaccaaa ccgttcaacc cgcgtgaact
     7261 gacgattcgt gcacgcaacc tgctgtcccg taccatgaat ctgggtactg tcagcgaaga
     7321 acgtcgtagc gttgaaagct acaagttcaa tggttgggaa ctggacatca acagccgttc
     7381 gttgatcggc cctgatggcg agcagtacaa gctgccgcgc agcgagttcc gcgccatgct
     7441 tcacttctgt gaaaacccag gcaaaattca gtcccgtgct gaactgctga agaaaatgac
     7501 cggccgtgag ctgaaaccac acgaccgtac tgtagacgtg acgatccgcc gtattcgtaa
     7561 acatttcgaa tctacgccgg atacgccgga aatcatcgcc accatccacg gtgaaggtta
     7621 tcgcttctgc ggtgatctgg aagattaatc ggcgttacca ccgtcaaaaa aaatggcgct
     7681 ttttagcgcc gtttttattt ttcaacctta tttccagata cgtaattgat cgtccattgt
     7741 aacttcttta ctggctttca ttttcggcag tgaaaacgca taccagtcga tattacgagt
     7801 cacaaacatc atgccggcca gcgccaccac cagcacactg gttcccaaca acagcgcgct
     7861 atcggcagag ttgagcagtc cccacatcac accatccagc aacaacagcg cgagggtaaa
     7921 caacatgctg ttgcgccagc ctttcaatac cgcctgcaaa taaataccgt tcattaacgc
     7981 cccaatcaga ctggcgatta tccatgccac ggtaaaaccg atatgttcag aaagcgccag
     8041 caagagcaaa taaaacatca ccaatgaaag ccccaccagc aaatattgca ttgggtgtaa
     8101 acgttgcgcg gtgagcgttt caaaaacaaa gaacgccata aaagtcagtg caatcagcag
     8161 aatggcgtac ttagtcgccc ggtccgttaa ttggtattga tcggctggcg tcgtcactgc
     8221 gacgctaaac gcggggaagt tttcccagcc ggtatcattg cctgaagcaa aacgctcacc
     8281 gagattatta gcaaaccagc tgctttgcca ttgcgccaga aaacctgact cgctaacttc
     8341 ccgtttggct ggcaaaaaat cacctaaaaa actgggatgc ggccagttgc tggttaaggt
     8401 catttcgcta ttacgcccgg caggcaccac agaaagatcg ccggtaccgc ttaaattcag
     8461 ggccatattc agcttcaggt tctgcttccg ccagtcccct tcgggtaaag ggatatgcac
     8521 gccctgcccg ccttgctcta acccggtgcc gggttcaatg gtcagcgccg ttccgttaac
     8581 ttcaggcgct ttcaccacac caataccacg cgcatccccg acgctaatca caataaaggg
     8641 cttgcctaag atgatatttg gcgcgttgag ctcgctaaga cgcgaaacat cgaaatcggc
     8701 ttttagcgtt aaatcactgt gccagacctg accggtataa atccctatct tgcgttcttc
     8761 cacgttctga ttgccatcaa ccatcaatga ctcaggtaac caaaaatgga taaaacttcg
     8821 tttccgctcc acggttttat cctcttcctg caccgtataa agctcggtca caggaatagc
     8881 gataagcggc ccaacgagtt tttgcggccc gctggtactt tggcgaatcg catcttccac
     8941 atcgctacgg taatcggcac gttcgacaat cacttgccga atcagcatta tcggaatcaa
     9001 caacagcaat actgcaccaa acaaggtggt cattttccag aacagggggg atttcaacat
     9061 agtcttctcc tttgcaatgg cagcaggata cgaaggctat gtgggaagaa tttgaagcta
     9121 tgtgaagtga cggtgaagtc gaagcgaggc cagcacgcca ccttcctgca cgttgcgcag
     9181 cgtgacttcg ccgttaaaca aacgggcgac ctcactgacg aacgccaacc ccagaccgct
     9241 gcttttttgc ccatttgcac gcggcaaaga gtaaaagcgt tcaaaaatac gtgacagcgc
     9301 gtagtcagga ataccactac cggtatccag caccttaagg gcgacgtgtt cctgatccac
     9361 ttcggcgctt agcgttatgc gaccgctctc gggggtaaaa tcgatggcgt tatccagtaa
     9421 attccccagc gcctgctcca gtaacgccgg ttcagcagca acattaacct cagtgggcat
     9481 aacatgtaga gtgatgtttt tttctgccaa ctgcacggtg cgcgcttcgc tgacgcggcg
     9541 aaataatgcc gccacatcaa caacagtcag aacgacttcc tgacgattct ccagtcttgc
     9601 ctggcgtagt aacgtttcta ccaatgcctg catacgcgca ttttgcgtca gaatgttgtc
     9661 ggtaaaacga gccaccactt ccggtggcgg accttcgcgt aaaatttccg ccgcgccacg
     9721 aatcgccgcc agtgggcttt ttagctcatg ggttaacgca taaacatact gctcaatata
     9781 gtttttccct tccagcttca cgcgcatact ttccagcgcc tgcgcaagct tacgcaactc
     9841 gctactgccg agttcaggaa gaggaacggg cttattgtca gtgacggaat cggcatagcg
     9901 agtgagccgg gcaatggagc ggttgatcca ccaaaccatg cctgcgccaa tcaccagtgc
     9961 aatccccagc aaaatggcgc tggcccataa aattcgccgc tcgctacgct taatgaccgg
    10021 agccatcgcc gcgttcggtt tgcctacgct caaaacgcca ataagccgcg agccgtccat
    10081 aatcggcgcg gcaacataca tcaccgaact ttcgggatcg gcaggatttt gcaacgtgct
    10141 gcgcgcacca tactgaccac gcaacgttag ccagacgtca ttccagcgcg aataatcctg
    10201 cccaacggct ttatttgccg aatcgaacaa tactttaccc tgcgaatcgg tcatatagac
    10261 atgatattca ttgcgcactt tgttaatgcc accgatattg gcgcgaaacg ggcgatgttg
    10321 tagctgatta aacgcctgcg ccagttgccc atgcgttggg tccccagaga gcaaatcggg
    10381 acgcgccagc tccgccagca acgttgcggt atcgattaac gtcccctccg ttgctcttcg
    10441 cacgcccggt ttaacttctt tgacaaaaat agccagtacg aaccaggccg ccaccgccac
    10501 cagtaaaaaa tagcccagca gcaaccgcat gccgatacgc attacaggcc cctcaggcta
    10561 tatcccatgc cgcgatgagt attaatcggt gaaagatcgg ggttgatggc gcgcagcttg
    10621 gcacgcaacg ttttaatgtg ggtatcaatg gtgcgatcgt aggtgtcctg cgcatcttcc
    10681 cacacgctat ccattagttg ctggcgggac cagacgcggc ctggcgactt gagtaacgtc
    10741 ttcagcaata aaaactcata ccgcgtcagc gttaatggcg tgtcaaacca gctgatctgc
    10801 gccgcgggtt cattcaattc aaaatgacca atacggatga cgggagacgg cgacgagaac
    10861 ttcttcaccc gacgcagtaa ggtgcgcacc cttgcgcaca cttcgcgggg tgaaaacggt
    10921 ttagccacat agtcgtcagc gccaatttcc agcccaagca ggcgatcgac ctcttcactt
    10981 cgggccgtca ggaacagtac aggtaacgcc ggatggagcg ccagtaactg gcggcataat
    11041 tcaaagccgc tgatatcagg caaaccaaca tcgagaatca tgacgtcggg agcctgctgg
    11101 cgagctttat ccagcaccgg caagcctcgc tcaaagacct cgacggcaaa accttcctgc
    11161 tgcaacatgt agaccagcgt gtcggctatc ccttgctcat cttccactaa ccagaccgtt
    11221 tcccgttgca taaatcgcct ctgttattgc cgccacggca tgacaggaac cgcgctaatc
    11281 gcgtttttcg gcgaaccttc tacaactttg tcggagtaag ccagataagc gagcgcgttg
    11341 cgtttggcgt cataaaagcg cacgacctgt aacgacttaa agaccaggga cgtgcgtttt
    11401 ttgaatacta cctcgccctg agctttgccg tttttaatac gatccgacag ttcaatcggc
    11461 ccgacttgct gacaagaaat agccgcatcg gaggtatctt ccgccagacc caatccccct
    11521 ttaatacccc cggttttcgc ccggctcaca taacaggtga catttttcac atcgggatcg
    11581 tcaaaggctt ccacaacaat tttgtgatcc gggccgatca ttttaaatac ggtgtcgacc
    11641 gaaccaatct cctctgcatg agccaatggc cccagcatta tcaggcttaa agacag
//
LOCUS       DAZKHF020000136        11539 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2201S31241
            SAMN43267800-rid25106413.denovo.137, whole genome shotgun sequence.
ACCESSION   DAZKHF020000136 DAZKHF020000000
VERSION     DAZKHF020000136.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267800
            Sequence Read Archive: SRR30307263
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 11539)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11539)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 11539)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350581.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350581.2
            Genome Coverage       :: 151x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:21:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,652
            CDSs (total)                      :: 5,557
            Genes (coding)                    :: 5,320
            CDSs (with protein)               :: 5,320
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 237
            CDSs (without protein)            :: 237
            Pseudo Genes (ambiguous residues) :: 0 of 237
            Pseudo Genes (frameshifted)       :: 102 of 237
            Pseudo Genes (incomplete)         :: 130 of 237
            Pseudo Genes (internal stop)      :: 52 of 237
            Pseudo Genes (multiple problems)  :: 41 of 237
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11539
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267800-rid25106413.denovo.137"
                     /serotype="O 26:H11"
                     /isolate="2201S31241"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-01"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            9..2434
                     /gene="ynfE"
                     /locus_tag="ACHOSC_RS14985"
                     /old_locus_tag="pgaptmp_002997"
                     /pseudo
     CDS             9..2434
                     /gene="ynfE"
                     /locus_tag="ACHOSC_RS14985"
                     /old_locus_tag="pgaptmp_002997"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416104.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="selenate/tellurate reductase subunit YnfE"
     gene            2495..4918
                     /gene="ynfF"
                     /locus_tag="ACHOSC_RS14990"
                     /old_locus_tag="pgaptmp_002998"
     CDS             2495..4918
                     /gene="ynfF"
                     /locus_tag="ACHOSC_RS14990"
                     /old_locus_tag="pgaptmp_002998"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416105.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="selenate/tellurate reductase subunit YnfF"
                     /protein_id="HGU4548898.1"
                     /translation="MKIHTTEALMKAEISRRSLMKTSALGSLALASSAFTLPFSQMVR
                     AAEAPVEEKAVWSSCTVNCGSRCLLRLHVKDDTVYWVESDTTGDDVYGNHQVRACLRG
                     RSIRRRMNHPDRLKYPMKRVGKRGEGKFERISWDEALDTISDNLRRILKDYGNEAVHV
                     LYGTGVDGGNITNSNVPYRLMNSCGGFLSRYGSYSTAQISAAMSYMFGANDGNSPDDI
                     ANTKLVVMFGNNPAETRMSGGGVTYYVEQARERSNARMIVIDPRYNDTAAGREDEWLP
                     IRPGTDGALACAIAWVLITENMVDQPFLDKYCVGYDEKTLPANAPRNAHYKAYILGEG
                     PDGIAKTPEWAAKITSIPAEKIIQLAREIGSAKPAYICQGWGPQRHSNGEQTSRAIAM
                     LSVLTGNVGINGGNSGVREGSWDLGVEWFPMLENPVKTQISVFTWTDAIDHGTEMTAT
                     RDGVRGKEKLDVPIKFLWCYASNTLINQHGDINHTHEVLQDDSKCEMIVGIDHFMTAS
                     AKYCDILLPDLMPTEQEDLISHESAGNMGYVILAQPATSAKFERKPIYWMLSEVAKRL
                     GPDVYQTFTEGRSQHEWVKYLHAKTKERNPEMPDYEEMKTTGIFKKKCPEEHYVAFRA
                     FREDPQANPLKTPSGKIEIYSERLAKIADTWELKKDEIIHPLPAYTPGFDGWDDPLRK
                     TYPLQLTGFHYKARTHSSYGNIDVLQQACPQEVWINPIDAQARGIRHGDTVRVFNNNG
                     EMLIAAKVTPRILPGVTAIGQGAWLKADMFGDRVDHGGSINILTSHRPSPLAKGNPSH
                     SNLVQIEKV"
     gene            4929..5546
                     /gene="ynfG"
                     /locus_tag="ACHOSC_RS14995"
                     /old_locus_tag="pgaptmp_002999"
     CDS             4929..5546
                     /gene="ynfG"
                     /locus_tag="ACHOSC_RS14995"
                     /old_locus_tag="pgaptmp_002999"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000213046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DMSO reductase like molybdoenzyme iron-sulfur
                     subunit YnfG"
                     /protein_id="HGU4548899.1"
                     /translation="MTTQYGFFIDSSRCTGCKTCELACKDFKDLGPEVSFRRIYEYAG
                     GDWQEDNGVWHQNVFAYYLSISCNHCDDPACTKVCPSGAMHKREDGFVVVDEDVCIGC
                     RYCHMACPYGAPQYNAEKGHMTKCDGCYSRVAEGKQPICVESCPLRALEFGPIEELRQ
                     KHGTLAAVAPLPRAHFTKPNIVIKPNANSRPTGDTTGYLANPEEV"
     gene            5548..6402
                     /gene="ynfH"
                     /locus_tag="ACHOSC_RS15000"
                     /old_locus_tag="pgaptmp_003000"
     CDS             5548..6402
                     /gene="ynfH"
                     /locus_tag="ACHOSC_RS15000"
                     /old_locus_tag="pgaptmp_003000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="selenate reductase membrane anchor subunit YnfH"
                     /protein_id="HGU4548900.1"
                     /translation="MGNGWHEWPLVIFTVLGQCVVGALIVSGIGWFAAKNDADRQRIV
                     RGMFFLWLLMGIGFIASVMHLGSPLRAFNSLNRIGASGLSNEIAAGSIFFAVGGLWWL
                     VAVIGKMPQALGKLWLLVSMALGVIFVWMMTCVYQIDTVPTWHNGYTTLAFFLTVLLS
                     GPILAAAILRAARVTFNTTPFAIISVLALIACAGVIVLQGLSLASIHSSVQQASALVP
                     DYASLQVWRVVLLCAGLGCWLCPLIRRREPHVAGLILGLILILGGEMIGRVLFYGLHM
                     TVGMAIAG"
     gene            6445..7059
                     /gene="dmsD"
                     /locus_tag="ACHOSC_RS15005"
                     /old_locus_tag="pgaptmp_003001"
     CDS             6445..7059
                     /gene="dmsD"
                     /locus_tag="ACHOSC_RS15005"
                     /old_locus_tag="pgaptmp_003001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707489.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Tat proofreading chaperone DmsD"
                     /protein_id="HGU4548901.1"
                     /translation="MTHFSQQDNFSVAARVLGALFYYAPESAEAAPLVAVLTSDGWET
                     QWPLPEASLAPLVTAFQTQCEETHAQAWQRLFVGPWALPSPPWGSVWLDRESVLFGDS
                     TLALRQWMREKGIQFEMKQNEPEDHFGSLLLMAAWLAENGRQTECEELLAWHLFPWST
                     RFLDVFIEKAEHPFYRALGELARLTLAQWQSQLLIPVAVKPLFR"
     gene            7218..8510
                     /gene="clcB"
                     /locus_tag="ACHOSC_RS15010"
                     /old_locus_tag="pgaptmp_003002"
     CDS             7218..8510
                     /gene="clcB"
                     /locus_tag="ACHOSC_RS15010"
                     /old_locus_tag="pgaptmp_003002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001296088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005247 - voltage-gated chloride channel
                     activity [Evidence IEA];
                     GO_process: GO:0006821 - chloride transport [Evidence
                     IEA];
                     GO_process: GO:0010447 - response to acidic pH [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="voltage-gated ClC-type chloride channel ClcB"
                     /protein_id="HGU4548902.1"
                     /translation="MHHLHIYPDLRTMFRRLLIATVVGILAAFAVAGFRHAMLLLEWL
                     FLNNDSGSLVNAATNLSPWRRLLTPALGGLAAGLLLMGWQKFTQQRPHAPTDYMEALQ
                     TDGQFDYAASLVKSLASLLVVTSGSAIGREGAMILLAALAASCFAQRFTPRQEWKLWI
                     ACGAAAGMAAAYRAPLAGSLFIAEVLFGTMMLASLGPVIISAVVALLVSNLINHSDAL
                     LYNVQLSVTVQARDYALIISTGVLAGLCGPLLLTLMNACHRGFVSLKLAPPWQLALGG
                     LIVGLLSLFTPAVWGNGYSTVQSFLTAPPLLMIIAGIFLCKLCAVLASSGSGAPGGVF
                     TPTLFIGLAIGMLYGRSLGLWFPDGEEITLLLGLTGMATLLAATTHAPIMSTLMICEM
                     TGEYQLLPGLLIACVIASVISRTLHRDSIYRQHTAQHS"
     gene            complement(8463..9158)
                     /gene="bioD"
                     /locus_tag="ACHOSC_RS15015"
                     /old_locus_tag="pgaptmp_003003"
     CDS             complement(8463..9158)
                     /gene="bioD"
                     /locus_tag="ACHOSC_RS15015"
                     /old_locus_tag="pgaptmp_003003"
                     /EC_number="6.3.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310326.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004141 - dethiobiotin synthase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0009102 - biotin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dethiobiotin synthase"
                     /protein_id="HGU4548903.1"
                     /translation="MLKRFFITGTDTSVGKTVVSRALLQALASQGKTVAGYKPVAKGS
                     KETPEGLRNKDALVLQSVSTIELPYEAVNPIALSEEESSVAHSCPINYTLISNGLANL
                     TDKVDHVVVEGTGGWRSLMNDLRPLSEWVVQEQLPVLMVVGIQEGCINHALLTAQAIA
                     NDGLPLIGWVANRINPGLAHYAEIIDVLGKKLPAPLIGELPYLPRAEQRELGQYIRLA
                     MLRSVLAVDRVTV"
     gene            complement(9283..10503)
                     /gene="mlc"
                     /locus_tag="ACHOSC_RS15020"
                     /old_locus_tag="pgaptmp_003004"
                     /gene_synonym="dgsA"
     CDS             complement(9283..10503)
                     /gene="mlc"
                     /locus_tag="ACHOSC_RS15020"
                     /old_locus_tag="pgaptmp_003004"
                     /gene_synonym="dgsA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar metabolism global transcriptional
                     regulator Mlc"
                     /protein_id="HGU4548904.1"
                     /translation="MVAENQPGHIDQIKQTNAGAVYRLIDQLGPVSRIDLSRLAQLAP
                     ASITKIVREMLEAHLVQELEIKEAGNRGRPAVGLVVETEAWHYLSLRISRGEIFLALR
                     DLSSKLVVEESQELALKDDSPLLDRIISHIDQFFIRHQKKLERLTSIAITLPGIIDTE
                     NGIVHRMPFYEDVKEMPLGEALEQHTGVPVYIQHDISAWTMAEALFGASRGARDVIQV
                     VIDHNVGAGVITDGHLLHAGSSSLVEIGHTQVDPYGKRCYCGNHGCLETIASVDSILE
                     LAQLRLNQSMSSMLHGQPLTVDSLCQAALRGDLLAKDIITGVGAHVGRILAIMVNLFN
                     PQKILIGSPLSKAADILFPVISDSIRQQALPAYSQHISVESTQFSNQGTMAGAALVKD
                     AMYNGSLLIRLLQG"
     gene            complement(10638..11531)
                     /gene="ynfL"
                     /locus_tag="ACHOSC_RS15025"
                     /old_locus_tag="pgaptmp_003005"
     CDS             complement(10638..11531)
                     /gene="ynfL"
                     /locus_tag="ACHOSC_RS15025"
                     /old_locus_tag="pgaptmp_003005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HGU4548905.1"
                     /translation="MNIELRHLRYFVAVAEELHFGRAAARLNISQPPLSQQIQALEQQ
                     IGARLLARTNRSVLLTAAGKQFLADSRQILSMVDDAAARAERLHQGEAGELRIGFTSS
                     APFIRAVSDTLSLFRRDYPDVHLQTREMNTREQIAPLIEGTLDMGLLRYTALPETLEH
                     AVIVHEPLMAMIPHDHPLANNPNVTLAELAKEPFVFFDPHVGTGLYDDILGLMRRYHL
                     TPVITQEVGEAMTIIGLVSAGLGVSILPASFKRVQLNEMRWVPIAEEDAVSEMWLVWP
                     KHHEQSPAARNFRIHLLNALR"
BASE COUNT         2804 a         2994 c         2953 g         2788 t
ORIGIN      
        1 agtgagttat gtccaaaaat gaccgaatgg tgggcatcag ccgcagaaca ctcgttaaat
       61 caaccgcgat aggttctctg gcgctggctg caggcggttt ttctttaccg ttcaccctgc
      121 gcagtgcagc ggcaacggta caacaggcca gcgaaaaagt gatctggggt gcctgttccg
      181 tcaactgtgg tagccgctgt gcacttcgtc tacatgttaa agataatgaa gtgacctggg
      241 tggaaactga caataccggc agcgatgagt acggcaacca tcaggtacgc gcctgtttgc
      301 gcggtcgctc catccgccgg cgtattaatc atcccgatcg cttgaattac ccaatgaaac
      361 gcgtgggtac acgcggtgaa ggcaaattcg aacggattag ctgggatgaa gccctggata
      421 ctatcgccag tagcctgaaa aaaaccgtcg aacaatatgg caatgaggct gtatatattc
      481 agtactcttc ggggattgtt ggcggcaata tgacccgctc ttcgccatca gcctcggcgg
      541 tcaaacgcct gatgaactgc tacggcggtt cactcaatca gtatggctcc tacagcaccg
      601 cgcaaatttc ctgtgccatg ccctacacct acggcagtaa tgatggcaac agcaccacgg
      661 atattgaaaa cagcaagctg gtcgtgatgt ttggtaacaa cccggcagaa acccgcatga
      721 gcggcggtgg catcacttat cttcttgaaa aagcgcgcga gaaatcgaac gccaaaatga
      781 ttgttatcga tccgcgatat accgatacgg ctgctggtcg tgaagatgaa tggctcccta
      841 ttcgcccggg caccgatgcc gcgctggttg cgggtattgc ctgggtattg attaacgaaa
      901 atctcgttga tcaacctttt ctcgataaat actgcgtcgg ctatgacgaa aaaaccttac
      961 ccgcagatgc acccaaaaat ggtcactata aagcctatat tcttggtgaa ggtgatgata
     1021 acacagcgaa aacgccgcag tgggcttcgc aaattaccgg tatcccggta gaccgtatca
     1081 tcaaactggc gcgtgaaatt ggcacagcaa aacccgctta tatctgccag ggctgggggc
     1141 cgcaacgcca ggcaaacggc gaactgactg cacgcgctat tgctatgtta cctattttga
     1201 cgggcaatgt cggcatcagc ggcggaaata gtggcgcgcg tgaatcgacc tataccatta
     1261 ccatagaacg cctgccggtg ctggataatc cggtcaaaac gtcaatctcc tgcttcagct
     1321 ggacagatgc tatcgatcat ggtccgcaaa tgacggcaat ccgcgacggc gtccgcggca
     1381 aagataaact ggatgtgccc attaagttca tctggaacta cgcgggaaat accctcgtta
     1441 atcagcattc tgacatcaac aaaacgcatg aaattctgca ggacgaatcg aaatgcgaaa
     1501 tgattgtgat catcgaaaac tttatgacct catcggcaaa atatgccgac attctgctgc
     1561 cagacctgat gaccgttgag caggaagata ttattcctaa cgactacgcc ggtaacatgg
     1621 gatatctcat tttcctccag cctgtcacca gcgaaaaatt cgaacgcaaa ccgatttact
     1681 ggatcctgag tgaagtcgcg aaacgtcttg gacctgacgt ctatcaaaag ttcacagaag
     1741 gtcgcacgca ggaacaatgg ttacaacatc tgtacgccaa aatgcttgcc aaagatccgg
     1801 cgttaccgtc ttacgacgaa ctgaaaaaat gggtatctat aagcgtaaag atcccaatgg
     1861 ccattttgtc gcctacaaag catttcgtga cgaccccgag gcaaatccac ttaaaacgcc
     1921 ttccggtaag attgaaattt attccagcag gctggcggaa attgcccgta cctgggaact
     1981 ggaaaaagat gaagtgataa gcccattgcc agtttatgcc tcaacctttg aaggctggaa
     2041 ctcccctgaa cgtagaacct tcccactgca actgtttggt ttccattaca aatcccgtac
     2101 tcactcgacc tacggcaata ttgatctcct gaaggctgcc tgccgtcagg aggtgtggat
     2161 caaccctata gatgcgcaga aacgtgggat tgccaacggc gatatggtgc gggtgtttaa
     2221 ccatcgtggc gaagttcggc taccagccaa agtaacacca cgtattctcc ctggagttag
     2281 cgctatgggc cagggagcct ggcacgaggc caatatgtct ggcgataaaa tcgaccatgg
     2341 cggctgtgtg aatacgctaa ccactctgcg cccttcacca ctggcgaagg gaaacccgca
     2401 gcacactaat ctggtcgaga tcgaaaaaat ataacccacg acaaccataa attctggcat
     2461 gacattttgt ttgaaaagca ataagtgagt aatgatgaaa atccatacca cagaggcgct
     2521 catgaaggct gaaatcagcc gtagaagtct gatgaaaacc tccgcacttg gcagtcttgc
     2581 gctggcaagc agtgctttca ctctgccatt ttcccaaatg gtccgggcgg cagaggctcc
     2641 ggtagaagag aaagcggtct ggagttcctg caccgttaac tgcgggagcc gctgtctgtt
     2701 acgtttgcat gtgaaagatg acaccgtgta ctgggtggag tctgatacga caggtgacga
     2761 cgtctacggt aaccatcagg ttcgagcgtg tttacgcggg cgctctatcc gccgacggat
     2821 gaatcatcct gataggttga aatatcccat gaagcgcgtc ggcaagcgcg gtgaaggtaa
     2881 atttgaacgg ataagttggg acgaagccct ggataccatc agtgataatc ttcggcggat
     2941 cctgaaagat tacggcaatg aggctgtaca tgtcctgtac ggaacaggcg tagatggcgg
     3001 aaacatcacc aactcaaacg tcccgtaccg tctgatgaac tcttgcggtg gttttctcag
     3061 tcgctatggc agctacagta ccgcacagat cagtgccgca atgagttata tgttcggtgc
     3121 caatgatggc aacagcccgg atgatatcgc caatacgaaa ctggtcgtta tgttcggaaa
     3181 taacccggca gaaacgcgga tgagcggcgg tggtgtcact tactacgtcg agcaagcccg
     3241 cgaacgttca aacgcacgca tgatcgtcat cgatccacgt tataacgaca ctgctgccgg
     3301 gcgtgaagat gaatggctgc ccattcgccc tggcaccgat ggcgcactgg cctgtgcgat
     3361 tgcctgggta ctgattactg aaaacatggt cgatcagcca tttctcgaca aatattgtgt
     3421 tggttacgat gaaaaaacgc tgcccgccaa cgcaccacgt aacgcgcatt ataaagccta
     3481 tattctgggc gaagggcctg acggcatagc taaaacgccg gaatgggcag caaaaatcac
     3541 cagcatcccg gcagaaaaaa ttatccagtt ggcacgagag atcggttcag caaaacctgc
     3601 ttatatttgt cagggttggg ggccacaacg acattccaac ggcgagcaaa cgtcccgcgc
     3661 tattgccatg ctttccgttc tcaccggtaa cgtcggcata aacggcggca actcaggtgt
     3721 acgcgaaggt agctgggatc tgggggtaga atggttcccg atgcttgaga atcctgttaa
     3781 aacgcagatt tccgtcttta catggacaga tgccatcgac catggtacgg aaatgaccgc
     3841 gacccgtgat ggtgttcgtg gaaaagaaaa actggatgtc cccatcaagt ttttatggtg
     3901 ctacgccagt aacacattga tcaatcaaca tggcgacatc aatcacaccc atgaggtgct
     3961 tcaggatgac agcaagtgcg agatgattgt tggcattgac cacttcatga cggcctcggc
     4021 taagtattgc gatatcctgt tgcccgacct gatgccaaca gagcaagaag accttatctc
     4081 tcatgaatct gcagggaata tgggctatgt gatcctcgcc caacccgcaa cctcagcaaa
     4141 atttgaacgc aaacccatct actggatgct gagtgaagtc gccaaacgct taggaccaga
     4201 cgtttatcaa acctttactg aaggtcgcag tcagcatgaa tgggtcaaat atctccatgc
     4261 gaaaacgaag gaacgtaacc ctgagatgcc cgactacgag gagatgaaaa cgaccgggat
     4321 ctttaagaaa aaatgcccgg aagaacacta cgtcgctttc cgcgcattcc gtgaagatcc
     4381 acaggcaaac ccgttgaaaa caccttcggg gaaaatcgaa atttattctg aacgactggc
     4441 gaagattgca gatacctggg aattgaaaaa agatgaaatt attcatcccc ttcctgcgta
     4501 taccccaggt tttgatggct gggacgatcc cctgcggaaa acctatccac tgcagttaac
     4561 gggcttccat tacaaagcgc gtacccactc cagctacggc aatattgatg tgttacagca
     4621 ggcctgccca caagaggtgt ggatcaaccc cattgatgct caggcacgcg gtatccgtca
     4681 tggcgatacc gtgcgggtat ttaacaataa tggagaaatg ctgattgccg caaaagtgac
     4741 tccgcgtatt ctgcctggcg ttaccgccat cgggcaaggt gcgtggctta aagcggatat
     4801 gtttggtgac cgggtcgatc acggcggcag tatcaatatt ctgacctctc accgcccttc
     4861 accgctggca aagggaaacc cgtcgcacag caatcttgtc cagatcgaaa aggtttaagg
     4921 agtagcccat gaccacacaa tatggatttt ttatcgattc cagccgctgt actggctgta
     4981 aaacctgcga actggcgtgc aaagatttta aagatcttgg cccggaagtc agtttccgcc
     5041 gtatttatga atacgctggc ggcgactggc aggaagataa tggcgtctgg caccagaacg
     5101 tgttcgctta ttatctctcc atctcctgta accattgcga tgaccccgca tgtacaaaag
     5161 tctgccccag cggcgcaatg cataagcgtg aagatggctt tgtggtggtt gatgaagatg
     5221 tttgtatcgg ctgccgctac tgccacatgg cttgtccgta cggcgcacca cagtacaatg
     5281 ctgaaaaagg gcacatgacg aagtgcgatg gttgttattc gcgcgtcgcc gaggggaaac
     5341 aacccatatg cgtcgaatcc tgcccgctgc gtgcacttga gttcggtcca attgaagagc
     5401 tgcgccagaa gcacggcact ctggctgccg tcgcgccact gccgcgcgcg catttcacaa
     5461 aacccaatat cgttatcaaa cctaacgcca acagccgccc gaccggcgat accactggtt
     5521 atctggctaa tccggaggaa gtgtaaaatg ggaaatggat ggcatgaatg gccactggtt
     5581 atctttaccg tattgggtca atgcgtagta ggcgcgctga ttgttagcgg aattggctgg
     5641 ttcgccgcca aaaatgacgc agaccgtcag cgtattgtcc gtggcatgtt tttcctctgg
     5701 ctgttgatgg gcatcgggtt tattgcctct gtcatgcacc tgggatcgcc actgcgggcg
     5761 tttaactcgc tgaatcgaat cggagcttct ggtctgagta acgagattgc tgccggttcc
     5821 atattttttg ccgttggcgg actatggtgg ctggtggcgg tcatcggtaa aatgccgcaa
     5881 gcgttgggga aactctggtt acttgtcagc atggcgctcg gcgtcatttt cgtctggatg
     5941 atgacctgcg tgtatcaaat cgacactgtg ccaacctggc ataacggtta cactacgctg
     6001 gctttcttcc tgacggtatt gttgagcggt ccaatacttg ctgccgcaat tctgcgggca
     6061 gcccgcgtta catttaatac cacaccattt gccatcatta gcgttctggc gctgatcgct
     6121 tgtgccgggg tgattgtgct gcaaggtttg tctctggcgt ctattcactc atccgtgcaa
     6181 caagccagcg cactggtacc agattacgcc tccttacagg tctggcgtgt ggtattgtta
     6241 tgcgcaggtc ttggatgctg gctttgcccg ctcattcgtc gccgtgaacc tcacgttgcc
     6301 ggacttattc tggggctaat cctcatactt ggcggtgaga tgatcgggcg tgtgctcttt
     6361 tatggcctgc acatgacagt aggaatggct attgcaggtt aacaaattgg tgcggggcaa
     6421 cccgcacatt tcaggatgtt aggaatgacc catttttcac agcaagataa tttttctgtc
     6481 gcggcgcgcg tgttgggcgc gctgttttat tacgctccag agagtgcgga agccgcacct
     6541 ctggttgcgg tactcaccag tgacggctgg gaaactcagt ggcctctacc agaagcgtca
     6601 ttagcgccgc tggtgacggc ttttcagaca cagtgtgaag agactcacgc ccaggcctgg
     6661 cagcgtttgt ttgtcggccc gtgggcactg ccgtctccgc cgtgggggtc ggtctggctg
     6721 gatcgcgaat ctgtgctgtt tggcgattca acattggcac ttcgtcagtg gatgcgcgag
     6781 aaaggcattc agtttgaaat gaagcaaaac gaaccggaag atcattttgg ttcactgctg
     6841 ttgatggctg cgtggttggc ggagaatggt cgccagacag aatgcgaaga attgctggca
     6901 tggcaccttt ttccgtggtc aacacgtttt cttgatgttt ttatcgaaaa agcagaacac
     6961 cctttctacc gtgcactggg tgaactggct cgcttaacgc tggcgcaatg gcaatcacaa
     7021 ctgttaattc ctgtcgcggt taaaccgctg tttcgatagg atcaccgtaa tattgccgga
     7081 tggtgacgat tacgcgcaat ccggcaataa taggttacag tgtcacgttt ttttatctct
     7141 taaagcacgc actgcttttg cggctggcct cttttgccgc aaaatagtcg cccgtgtttc
     7201 attgcccatt tctgctcatg catcatctac acatctatcc ggatctgcgc actatgttcc
     7261 gccgtctgct tatcgcaaca gtcgtcggta ttctcgcggc ctttgccgtt gccgggtttc
     7321 gtcatgcgat gctgctactg gagtggttgt tcctcaataa tgactccggc agtctggtca
     7381 atgcagcgac aaacctttcc ccctggcgac ggttgctaac tccggcgctc ggcggactgg
     7441 cggcgggttt gttgctgatg ggctggcaga aatttaccca acaacgccct catgcgccga
     7501 ccgattacat ggaagcgttg caaaccgatg gacagttcga ttacgcagca agcctggtta
     7561 aatcgcttgc ctctctgctg gtagtaacca gcggcagtgc aattggtcgc gaaggtgcga
     7621 tgattctttt agctgccctt gccgcctcct gttttgccca acgttttacg ccacgccagg
     7681 agtggaaatt atggatcgcc tgtggggccg cggcgggaat ggctgcggcc tatcgtgccc
     7741 cgcttgctgg cagtttattt atagccgaag tgctgtttgg cactatgatg ttggcctctc
     7801 tcggcccggt gattatttcc gccgtcgtgg cattgctggt tagcaatctg attaatcata
     7861 gcgacgcgtt actctacaac gtacaactct cagtgacggt tcaggctcgt gactatgcgc
     7921 tgattatcag tacaggtgtg ctggcaggtc tgtgcggacc actgttgtta acgttaatga
     7981 acgcctgtca tcgtggattt gtgagtctca aacttgcgcc gccctggcaa ctggcactag
     8041 gcgggttgat cgtgggtctg ctttccctgt tcacacctgc agtgtggggc aacggctata
     8101 gcaccgtaca atccttttta accgccccac cactgttaat gatcattgcc gggatcttcc
     8161 tttgtaaact gtgtgccgtg ctggcgagta gcggttccgg cgcacccggt ggtgtcttta
     8221 caccgacgct atttatcggt cttgccattg gcatgttgta tggtcgtagc ctgggattat
     8281 ggttccctga tggcgaagaa attacgcttt tactcggatt gaccgggatg gcgacactgt
     8341 tggcggcaac cacgcacgcg ccgattatgt cgacgttgat gatatgtgaa atgaccgggg
     8401 agtatcagct actccccggt ttattgattg cctgcgtcat tgcgtcggta atttcgcgga
     8461 cgttacaccg tgactctatc taccgccagc acactgcgca gcatagctaa gcggatgtat
     8521 tgccccagtt ctcgctgttc ggcgcgcggc agataaggca gttcaccaat gagcggtgcc
     8581 ggaagttttt tacccagcac atcaatgatt tccgcataat gcgccagtcc tgggttgatt
     8641 cggttagcca cccagccaat gagcggcagc ccgtcgttgg caatcgcctg agctgttagc
     8701 agtgcatggt taatgcaacc ttcctgaata ccgacaacca tcaacaccgg caactgttcc
     8761 tgcaccaccc attcagagag tggacgcaaa tcattcatca gactgcgcca gccgccagtc
     8821 ccttctacca cgacatgatc gactttatcg gtcaggtttg ccaggccgtt tgaaatgagg
     8881 gtgtaattga ttgggcaact gtgcgccacg ctactttctt cttcgcttaa cgcgatagga
     8941 ttaactgctt cataaggcag ttcgatggtt gaaacactct gcaacaccag ggcatcttta
     9001 ttacgcagcc cttcgggtgt ctctttgctc cccttcgcta cgggtttata tcccgcaacc
     9061 gtttttccct gggaggctaa cgcttgtagc aatgcgcggg aaaccaccgt tttccctaca
     9121 gaagtgtctg taccggtaat aaagaaacgc ttcagcatca ctaactccac cgttatgctt
     9181 cacaaatata aaccaggaaa ataattaacc ttgaaagtct aagttatgct ttcctggccc
     9241 aaattgagat agcgcaaatt ttggtagaac agttaaaaaa tgttaaccct gcaacagacg
     9301 aatcaacaaa gaaccgttat acatcgcgtc ttttaccagt gcagcgcctg ccatcgtgcc
     9361 ctggttagaa aactgagtac tctcaacgct gatgtgctga ctatacgcag gaagggcctg
     9421 ctgacggatg ctgtctgaga tgaccgggaa gaggatatct gccgctttac ttaacggtga
     9481 gccaatcagt attttttgtg ggttaaataa attcaccatg atggcaagaa tgcgccctac
     9541 atgcgcgccc accccggtaa tgatgtcttt tgccagcaga tcgccgcgca atgccgcctg
     9601 acacaatgag tccacggtta acggttgtcc atgtaacatc gagctcatgg attgattaag
     9661 acgcagctgt gccagctcaa gaatactgtc cacactggcg atggtttcga ggcagccgtg
     9721 attcccgcaa taacagcgtt tcccatacgg gtcgacctgt gtgtggccta tttccacgag
     9781 actactgctg cctgcgtgta gcagatgacc atcggtaatg acgcccgccc ccacgttgtg
     9841 atcgataacc acctgaatca catcgcgcgc cccgcgtgag gcaccaaaca aggcctctgc
     9901 catcgtccat gcgctgatat catgctgaat ataaaccggt acgccggtat gctgctccag
     9961 cgcctcgccg agcggcatct cttttacatc ctcgtagaac ggcatgcgat gtacaatacc
    10021 attttccgta tcaataattc ccggcaaggt tatggcaatc gaagttagac gctcaagttt
    10081 tttctggtgg cgaataaaaa actgatcgat atgggaaatg atacgatcca gcaatggcga
    10141 gtcatctttt aacgccagtt cctgcgactc ttccaccacc agtttgctgc tcagatcgcg
    10201 cagagcaagg aaaatctccc cgcgactaat gcgcagagaa agatagtgcc aggcttcagt
    10261 ttcaaccacc agccccaccg ccggacggcc acggttcccc gcttctttga tttccagctc
    10321 ttgcaccagg tgtgcttcga gcatctcacg gacaatttta gtgatactgg caggagccag
    10381 ctgcgccaga cgggaaagat cgatacgcga gactggacca agctgatcaa tcaggcgata
    10441 aaccgcgccc gcgttggtct gctttatttg atcaatgtgc ccaggctggt tttcagcaac
    10501 caccgcatac tccctatatt ttcgcgctcc gaaataatct gtaggctatg gtgaagcact
    10561 tcaatacgtg tcgtcaaatt tttacttagg catgtgatta acagcacatt tttcgggctt
    10621 tttcgctgaa atttccctca cctgagagca ttcagcagat gaatacgaaa gttacgcgca
    10681 gccggacttt gttcatgatg tttcggccag accaaccaca tttcagaaac cgcatcctct
    10741 tcagcaatcg gcacccagcg catttcgttg agctgaacac gtttaaatga agcaggcaaa
    10801 attgaaacac ccagaccggc ggaaaccaga ccgatgatgg tcattgcctc gcccacctcc
    10861 tgagtgatga cgggcgtcaa atggtaacgt cgcatcagcc cgagaatatc gtcatacagc
    10921 cctgtcccga cgtgcggatc aaaaaagacg aagggttctt tcgccagttc agccagcgtt
    10981 acattcgggt tatttgccag gggatgatcg tgcgggatca tcgccataag cggttcatgg
    11041 acgatgactg cgtgttcaag cgtctccggt aacgctgtgt aacgcagcaa tcccatatcc
    11101 agcgttcctt caatgagcgg agcgatttgc tcacgagtgt tcatttcgcg ggtttgtaaa
    11161 tggacatcgg gataatcacg gcgaaacagc gataacgtat cggacaccgc ccgaataaaa
    11221 ggagcggacg aagtaaaacc aatgcgcaac tcccccgctt ccccctgatg cagtctttca
    11281 gcgcgagcgg cagcgtcatc taccatagac aggatttgcc gactatctgc aagaaactgt
    11341 tttcctgctg ccgtcagcaa tacactgcga ttagttcgtg ccagcagtcg ggcaccaatt
    11401 tgttgctcca gtgcctgaat ctgctgactt agcggtggtt gcgaaatatt caggcgggca
    11461 gcggcgcgcc cgaaatgcag ctcttccgca acagcaacaa agtaacgcag atgacgaagt
    11521 tcaatattca tatttaaaa
//
LOCUS       DAZKHF020000137        11508 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2201S31241
            SAMN43267800-rid25106413.denovo.138, whole genome shotgun sequence.
ACCESSION   DAZKHF020000137 DAZKHF020000000
VERSION     DAZKHF020000137.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267800
            Sequence Read Archive: SRR30307263
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 11508)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11508)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 11508)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350581.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350581.2
            Genome Coverage       :: 151x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:21:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,652
            CDSs (total)                      :: 5,557
            Genes (coding)                    :: 5,320
            CDSs (with protein)               :: 5,320
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 237
            CDSs (without protein)            :: 237
            Pseudo Genes (ambiguous residues) :: 0 of 237
            Pseudo Genes (frameshifted)       :: 102 of 237
            Pseudo Genes (incomplete)         :: 130 of 237
            Pseudo Genes (internal stop)      :: 52 of 237
            Pseudo Genes (multiple problems)  :: 41 of 237
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11508
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267800-rid25106413.denovo.138"
                     /serotype="O 26:H11"
                     /isolate="2201S31241"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-01"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            complement(135..908)
                     /gene="uxuR"
                     /locus_tag="ACHOSC_RS15030"
                     /old_locus_tag="pgaptmp_003006"
     CDS             complement(135..908)
                     /gene="uxuR"
                     /locus_tag="ACHOSC_RS15030"
                     /old_locus_tag="pgaptmp_003006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000833692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Uxu operon transcriptional regulator"
                     /protein_id="HGU4548906.1"
                     /translation="MKSATSAQRPYQEVGAMIRDLIIKTPYNPGERLPPEREIAEMLD
                     VTRTVVREALIMLEIKGLVEVRRGAGIYVLDNSGSQNTDSPDANVCNDAGPFELLQAR
                     QLLESNIAEFAALQATREDIVKMRQALQLEERELASSAPGSSESGDMQFHLAIAEATH
                     NSMLVELFRQSWQWRENNPMWIQLHSHLDDSLYRKEWLGDHKQILAALIKKDARAAKL
                     AMWQHLENVKQRLLEFSNVDDIYFDGYLFDSWPLDKVDV"
     gene            complement(1123..2583)
                     /gene="uxuB"
                     /locus_tag="ACHOSC_RS15035"
                     /old_locus_tag="pgaptmp_003007"
     CDS             complement(1123..2583)
                     /gene="uxuB"
                     /locus_tag="ACHOSC_RS15035"
                     /old_locus_tag="pgaptmp_003007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructuronate reductase"
                     /protein_id="HGU4548907.1"
                     /translation="MTTIVDSNLPVARPSWEHSRLESRIVHLGCGAFHRAHQALYTHH
                     LLESTDSDWGICEVNLMPGNDRVLIENLKKQQLLYTVAEKGAESTELKIIGSMKEALH
                     PEIDGCEGILNAMARPQTAIVSLTVTEKGYCADAASGQLDLNNPLIKHDLENPTAPKS
                     AIGYIVEALRLRREKGLKAFTVMSCDNVRENGHVAKVAVLGLAQARDPQLAAWIEENV
                     TFPCTMVDRIVPAATPETLQEIADQLGVYDPCAIACEPFRQWVIEDNFVNGRPDWDKV
                     GAQFVADVVPFEMMKLRMLNGSHSFLAYLGYLGGYETIADTMTNPDYRKAAFSLMMQE
                     QAPTLSMPEGTDLNAYATLLIERFSNPSLRHRTWQIAMDGSQKLPQRLLDPVRLHLQN
                     GGSWRHLALGVAGWMRYTQGVDEQGNAIDVVDPMLAEFQKVNAQYQGADRVKALLGLS
                     GIFADDLPQNADFVGAVTAAYQQLCERGARECVAAL"
     gene            complement(2664..3848)
                     /gene="uxuA"
                     /locus_tag="ACHOSC_RS15040"
                     /old_locus_tag="pgaptmp_003008"
     CDS             complement(2664..3848)
                     /gene="uxuA"
                     /locus_tag="ACHOSC_RS15040"
                     /old_locus_tag="pgaptmp_003008"
                     /EC_number="4.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709912.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008927 - mannonate dehydratase activity
                     [Evidence IEA];
                     GO_process: GO:0006064 - glucuronate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannonate dehydratase"
                     /protein_id="HGU4548908.1"
                     /translation="MEQTWRWYGPNDPVSLADVRQAGATGVVTALHHIPNGEVWSVEE
                     ILKRKAIVEDAGLVWSVVESVPIHEDIKTHTGNYEQWIANYQQTLRNLAQCGIRTVCY
                     NFMPVLDWTRTDLEYVLPDGSKALRFDQIEFAAFEMHILKRPGAEADYTEEEIAQAAV
                     RFATMSDEDKARLTRNIIAGLPGAEEGYTLDQFRKHLELYKDIDKAKLRENFAVFLKA
                     IIPVAEEVGVRMAVHPDDPPRPILGLPRIVSTIEDMQWMVDTVNSMANGFTMCTGSYG
                     VRADNDLVDMIKQFGPRIYFTHLRSTMREDNPKTFHEAAHLNGDVDMYEVVKAIVEEE
                     HRRKAEGKEDLIPMRPDHGHQMLDDLKKKTNPGYSAIGRLKGLAEVRGVELAIQRAFF
                     SR"
     gene            4300..4392
                     /locus_tag="ACHOSC_RS15045"
                     /old_locus_tag="pgaptmp_003009"
                     /pseudo
     CDS             4300..4392
                     /locus_tag="ACHOSC_RS15045"
                     /old_locus_tag="pgaptmp_003009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313307.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(4635..5537)
                     /gene="fimH"
                     /locus_tag="ACHOSC_RS15050"
                     /old_locus_tag="pgaptmp_003010"
     CDS             complement(4635..5537)
                     /gene="fimH"
                     /locus_tag="ACHOSC_RS15050"
                     /old_locus_tag="pgaptmp_003010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbria D-mannose specific adhesin FimH"
                     /protein_id="HGU4548909.1"
                     /translation="MKRVITLFAVLLMGWSVNAWSFACKTANGTAIPIGGGSANVYVN
                     LAPAVNVGQNLVVDLSTQIFCHNDYPETITDYVTLQRGSAYGSVLSNFSGTVKYSGSS
                     DPFPTTSETPRVVYNSRTDKPWPVALYLTPVSSAGGVAIKAGSLIAVLILRQTNNYNS
                     DDFQFVWNIYANNDVVVPTGGCDVSARDVTVTLPDYPGSVPIPLTVYCAKSQNLGYYL
                     SGTTVDAGNSIFTNTASFSPAQGVGVQLTRNGTIIPANNTVSLGAVGTSAVSLGLTAN
                     YARTGGQVTAGNVQSIIGVTFVYQ"
     gene            complement(5557..6060)
                     /gene="fimG"
                     /locus_tag="ACHOSC_RS15055"
                     /old_locus_tag="pgaptmp_003011"
     CDS             complement(5557..6060)
                     /gene="fimG"
                     /locus_tag="ACHOSC_RS15055"
                     /old_locus_tag="pgaptmp_003011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbria minor subunit FimG"
                     /protein_id="HGU4548910.1"
                     /translation="MKWRKRGYLLAAILALASATIQAADVTITVNGKVVAKPCTVSTT
                     NATVDLGDLYSFSLMSAGAASAWHDVALELTNCPVGTSRVTASFSGAADSTGYYKNQG
                     TAQNIQLELQDDSGNTLNTGATKTVQVDDSSQSAHFPLQVRALTVNGGATQGTIQAVI
                     SITYTYS"
     gene            complement(6073..6603)
                     /gene="fimF"
                     /locus_tag="ACHOSC_RS15060"
                     /old_locus_tag="pgaptmp_003012"
     CDS             complement(6073..6603)
                     /gene="fimF"
                     /locus_tag="ACHOSC_RS15060"
                     /old_locus_tag="pgaptmp_003012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbria minor subunit FimF"
                     /protein_id="HGU4548911.1"
                     /translation="MRNKPFYLLCAFLWLAVSHALAADSTITIRGYVRDNGCSVAAES
                     TNFTVDLMENAAKQFNNIGATTPVVPFRILLSPCGNAVSAVKVGFTGVADSHNANLLA
                     LENTVSAASGLGIQLLNEQQNQIPLNAPSSAISWTTLTPGKPNTLNFYARLMATQVPV
                     TAGHINATATFTLEYQ"
     gene            complement(6613..9249)
                     /gene="fimD"
                     /locus_tag="ACHOSC_RS15065"
                     /old_locus_tag="pgaptmp_003013"
     CDS             complement(6613..9249)
                     /gene="fimD"
                     /locus_tag="ACHOSC_RS15065"
                     /old_locus_tag="pgaptmp_003013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial usher FimD"
                     /protein_id="HGU4548912.1"
                     /translation="MSYLNLRLYQRNTQCLHIRKHRLAGFFVRLFVACAFAAQAPLSS
                     AELYFNPRFLADDPQAVADLSRFENGQELPPGTYRVDIYLNNGYMATRDVTFNTGDSE
                     QGIVPCLTRAQLASMGLNTASVAGMNLLADDACVPLTTMVQDATAHLDVGQQRLNLTI
                     PQAFMSNRARGYIPPELWDPGINAGLLNYNFSGNSVQNRIGGNSHYAYLNLQSGLNIG
                     AWRLRDNTTWSYNSSDRSSGSKNKWQHINTWLERDIIPLRSRLTLGDGYTQGDIFDGI
                     NFRGAQLASDDNMLPDSQRGFAPVIHGIARGTAQVTIKQNGYDIYNSTVPPGPFTIND
                     IYAAGNSGDLQVTIKEADGSTQIFTVPYSSVPLLQREGHTRYSITAGEYRSGNAQQEK
                     PRFFQSTLLHGLPAGWTIYGGTQLADRYRAFNFGIGKNMGALGALSVDMTQANSTLPD
                     DSQHDGQSVRFLYNKSLNESGTNIQLVGYRYSTSGYFNFADTTYSRMNGYNIETQDGV
                     IQVKPKFTDYYNLAYNKRGKLQLTVTQQLGRTSTLYLSGSHQTYWGTSNVDEQFQAGL
                     NTAFEDINWTLSYSLTKNAWQKGRDQMLALNVNIPFSHWLRSDSKSQWRHASASYSMS
                     HDLNGRMTNLAGVYGTLLEDNNLSYSVQTGYAGGGDGNSGSTGYATLNYRGGYGNANI
                     GYSHSDDIKQLYYGVSGGVLAHANGVTLGQPLNDTVVLVKAPGAKDAKVENQTGVRTD
                     WRGYAVLPYATEYRENRVALDTNTLADNVDLDNAVANVVPTRGAIVRAEFKARVGIKL
                     LMTLTHNNKPLPFGAMVTSESSQSSGIVADNGQVYLSGMPLAGKVQVKWGEEENAHCV
                     ANYQLPPESQQQLLTQLSAECR"
     gene            complement(9316..10041)
                     /gene="fimC"
                     /locus_tag="ACHOSC_RS15070"
                     /old_locus_tag="pgaptmp_003014"
     CDS             complement(9316..10041)
                     /gene="fimC"
                     /locus_tag="ACHOSC_RS15070"
                     /old_locus_tag="pgaptmp_003014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418736.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbria chaperone FimC"
                     /protein_id="HGU4548913.1"
                     /translation="MSNKNVNVRKSQEITFCLLAGILMFMAMMVAGRAEAGVALGATR
                     VIYPAGQKQVQLAVTNNDENSTYLIQSWVENADGVKDGRFIVTPPLFAMKGKKENTLR
                     ILDATNNQLPQDRESLFWMNVKAIPSMDKSKLTENTLQLAIISRIKLYYRPAKLALPP
                     DQAAEKLRFRRSANSLTLINPTPYYLTVTELNAGTRVLENALVPPMGESTVKLPSDAG
                     SNITYRTINDYGALTPKMTGVME"
     gene            complement(10078..10617)
                     /gene="fimI"
                     /locus_tag="ACHOSC_RS15075"
                     /old_locus_tag="pgaptmp_003015"
     CDS             complement(10078..10617)
                     /gene="fimI"
                     /locus_tag="ACHOSC_RS15075"
                     /old_locus_tag="pgaptmp_003015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418735.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HGU4548914.1"
                     /translation="MKRKRLFLLASLLPMFALAGNKWNTTLPGGNMQFQGVIIAETCR
                     IEAGDKQMTVNMGQISSNRFHAAGEDSAPVPFVIHLRECSTVVSERVGVAFHGVADGK
                     NPDVLSVGEGPGIATNIGVALFDDEGNLVPINRPPANWKRLYSGSTSLHFIAKYRATG
                     RRVTGGIANAQAWFSLTYQ"
     gene            complement(10682..11230)
                     /gene="fimA"
                     /locus_tag="ACHOSC_RS15080"
                     /old_locus_tag="pgaptmp_003016"
     CDS             complement(10682..11230)
                     /gene="fimA"
                     /locus_tag="ACHOSC_RS15080"
                     /old_locus_tag="pgaptmp_003016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbrial major subunit FimA"
                     /protein_id="HGU4548915.1"
                     /translation="MKIKTLAIVVLSALSLSSAAALADTTTVNGGTVHFKGEVVNAAC
                     AVDAGSVDQTVQLGQVRTASLKQAGATSSAVGFNIQLNDCDTTVATKAAVAFLGTAID
                     ATRTDVLALQSSAAGSATNVGVQILDRTGNALTLDGATFSAQTTLNNGTNTIPFQARY
                     YAIGEATPGAANADATFKVQYQ"
BASE COUNT         2781 a         3040 c         2770 g         2917 t
ORIGIN      
        1 agaagaatga gtactaatat tcaggttata cagatattta aacaataccc tgcaatttac
       61 aaagcaatac tcattgcgac ttcttaattt aagaaagcgc aaggaacgtt tacccttgcg
      121 cttattataa taagtcagac gtcgacttta tccagcggcc atgaatcaaa cagatagccg
      181 tcaaaataaa tatcgtcaac gttcgagaat tccagcagac gttgcttaac gttttccaga
      241 tgctgccaca ttgccagctt cgccgctcgg gcatcttttt tgattaacgc ggcgaggatc
      301 tgtttgtgat cgcccaacca ctctttgcga tacaggctgt catccagatg gctgtgcaac
      361 tggatccaca ttggattgtt ttcccgccac tgccaggact gacggaataa ctccaccagc
      421 atgctgttat gcgttgcttc ggcaatagcg agatggaact gcatgtcacc gctttcgctg
      481 ctgcccggcg cactggaagc cagttcacgc tcttccagtt gcaatgcctg acgcattttg
      541 acgatatctt cgcgggtagc ctgcaaagcg gcaaactcgg cgatgttgct ctccaataac
      601 tgccgcgcct gtaacagctc aaaaggaccg gcatcgttgc agacgttggc atccggactg
      661 tctgtgttct ggctgcctga gttatcaaga acatagatac cggcaccccg gcgtacttcc
      721 accagccctt tgatctccag catgatcagc gcttcacgta ccaccgtccg cgtgacatca
      781 agcatttctg caatttcacg ctccggcggc agccgttcgc caggattgta cggcgtcttt
      841 atgatcagat cgcggatcat cgccccgact tcctggtaag gtctttgcgc agaggtggca
      901 gatttcatgg tgttgtgtcc gttaggtgcg gttaatcaaa tttttgccgg atgcggcgtg
      961 aacgccttat ccggcctaca acgggcaatt ttacggcccc cgttaatctc aaactatacc
     1021 attagcactg gaagttggca cgtacaggtg ggcaaaaaaa cgaaaactgg tcaaccaaat
     1081 atcagtttga ttgaccagta agtctgtagg gtaatcagtt agttacagcg cagccacaca
     1141 ctcgcgcgca ccgcgttcgc acagctgctg atatgccgcc gtcactgcgc caacaaagtc
     1201 ggcattctgc ggcagatcat cggcaaaaat accgctcagg cccagcagcg ctttcacgcg
     1261 gtctgcgccc tgatactgcg cgttgacctt ctggaactcc gccagcatcg ggtcgaccac
     1321 gtcaatggca ttaccctgct catccacgcc ctgggtgtaa cgcatccagc cagccacgcc
     1381 cagcgccagg tgacgccagc tgccgccgtt ttgcaggtgc agacgcaccg ggtccagcag
     1441 acgctgcggt aacttctggc tgccgtccat cgcaatctgc caggtacggt gacgcagaga
     1501 cgggttgctg aaacgctcga tcagcagcgt cgcataggcg ttcaggtccg taccttccgg
     1561 catcgacagc gttggcgctt gttcctgcat catcaaggag aaggccgctt tgcgataatc
     1621 cgggttagtc atggtgtcgg caatggtttc atagccgccg aggtaaccga ggtatgccag
     1681 gaaagagtgg ctgccgttca gcatacgcag cttcatcatt tcgaacggca caacgtctgc
     1741 aacgaactgt gcgcccactt tatcccagtc cgggcgaccg ttaacgaagt tatcttcaat
     1801 cacccactga cggaacggtt cgcaggcaat ggcgcacggg tcgtaaacac ccagctggtc
     1861 agcaatttcc tgcaaggttt ctggcgtcgc cgccggaacg atgcggtcaa ccatggtgca
     1921 cgggaaggtg acgttttctt caatccatgc cgccagctgc gggtcacgcg cctgagccag
     1981 tcccagtacc gcgaccttcg ccacatggcc gttttcacgc acgttatcgc aggacatcac
     2041 cgtaaacgct ttcaaccctt tttcacgacg cagccgcagg gcttcgacga tgtaaccaat
     2101 cgcggacttc ggcgcagtcg ggttttccag atcgtgcttg atcagtgggt tattgagatc
     2161 cagctgaccg cttgccgcat cagcgcagta gcctttttcc gtgactgtca gagagacaat
     2221 cgccgtctgc ggacgcgcca tcgcattgag aataccttcg cagccgtcga tttccggatg
     2281 cagcgcttct ttcatcgaac cgataatttt cagctcggtg ctttccgcgc ctttttccgc
     2341 cacggtgtac agcagttgct gttttttcag gttttcgatc agcacgcggt cgttgcctgg
     2401 catcaggtta acttcgcaga taccccagtc gctgtcggtg ctttccagca ggtgatgggt
     2461 atacagcgcc tggtgcgcgc ggtgaaacgc cccgcaaccg agatgcacaa tgcgtgattc
     2521 cagacgagaa tgctcccatg acgggcgggc aaccggcaga ttgctgtcaa caatagtagt
     2581 catcgtaaac tccagaatcc gtcttcagag agtgacggca aggaagaagg aaccggcacg
     2641 gcgcgcagcc atgccggtgg gtattaacgg ctaaagaaag cgcgctggat cgccagttcg
     2701 acaccgcgaa cttcggccag gcctttcaga cgaccaattg cggagtaacc tgggttggtt
     2761 ttcttcttca ggtcgtccag catctgatga ccgtggtccg gacgcatcgg gatcaggtct
     2821 tctttgcctt ccgctttacg acggtgttct tcttcaacaa tcgctttcac cacttcgtac
     2881 atatcaacgt caccgttcag gtgcgccgct tcgtggaagg ttttcgggtt atcttcacgc
     2941 atggtggagc gcagatgggt gaagtaaata cgcggaccga actgcttgat catatcaacc
     3001 agatcgttgt cagcacgcac gccgtaggaa ccggtgcaca tggtgaaacc gttcgccatg
     3061 ctgtttacgg tatcaaccat ccactgcata tcttcaatgg tggaaacaat gcgcggcagg
     3121 ccaaggatcg ggcgcggcgg atcgtccggg tgaaccgcca tacgcacgcc aacttcttca
     3181 gcaactggaa taatcgcttt caggaagacg gcaaagtttt cgcgcagctt cgctttatcg
     3241 atatctttgt acagctccag gtgcttacgg aactggtcga gggtataccc ttcttccgcg
     3301 cccggcagac cggcaatgat gttacgcgtc agacgcgctt tgtcttcgtc gctcatagtg
     3361 gcgaagcgaa cagcagcctg agcaatttct tcttcggtgt aatccgcttc cgcgcctgga
     3421 cgtttcagga tatgcatttc gaatgcagcg aattcgatct ggtcgaagcg cagagctttg
     3481 gagccgtctg gcagcacgta ttcgaggtca gtacgggtcc agtcgagcac cggcatgaag
     3541 ttgtagcaca cggtgcgaat gccgcactgc gccaggttgc gcagagtctg ctgatagtta
     3601 gcgatccact gctcatagtt gccagtgtgg gttttgatat cttcgtgaat tggtacgctt
     3661 tctacgacag accacaccag gcctgcgtct tcaacgatcg ccttgcgttt gaggatctct
     3721 tctacggacc atacttcgcc gttcgggata tggtgcagcg cggtaaccac gccagttgcg
     3781 cccgcctgac ggacatcagc taaagaaacc ggatcgtttg ggccgtacca gcgccaggtc
     3841 tgttccatga tttttcctct ttcacatagt gggttccaca gcaaagagac gcggaaccct
     3901 ggagttgttt aggtatccgg atgaacaacc agaccgcagc cgtggcgttg catcggatga
     3961 gatggcgtat aagttctacc ttttggtaag acatgtcaac ccgaattctg aaattggtta
     4021 accacatcac aagaatttca ctctcatggt tgttgctgca aattgcagca aagggtaatc
     4081 gatggttatt gatcagagga tgtgacattc atcgcaacaa tggttgacca atttacataa
     4141 catgtcgccc aaaataacaa cggttcaacc aggtaaggta aaacgttatg catgtgctta
     4201 acattctctg ggtggtattc ggcattggcc tgatgctggt actgacaaag accgcaggtt
     4261 cagacacatt cgctgcatta gcaatgcctc ttagataatt ttgacctgtc agtaaaagac
     4321 acgttgaaaa cctggggtct gctggagctg gtcaactccg tggttgggct gattattagc
     4381 aaggtagcgt aaaagaacac cagagcctgc caatggcagg ctctgactga tgacaaagtc
     4441 taaaaatgtg cccggacagt cccctcgccc ctccggggag agggttaggg tgaggggaac
     4501 agaccagcaa agtgcgcttt gtttatgcca gatgcgacgc tgacgcgtct tatctggcct
     4561 acaaagggct aacgtgcagg tttttagctt caggtaatat tgcgtaactg cattagcaat
     4621 gccctgtgat ttctttattg ataaacaaaa gtcacgccaa taatcgattg cacattccct
     4681 gcagtcacct gccctccggt acgtgcgtaa tttgccgtta atcccagact taccgccgaa
     4741 gtccctactg ctcctaacga taccgtgtta ttcgctggaa taatcgtacc gttgcgcgtc
     4801 aactgtacgc cgacgccctg cgctggtgaa aacgacgcgg tattggtgaa aatcgagttg
     4861 cccgcatcta cggttgtgcc ggagaggtaa taccccagat tttggctttt cgcacaataa
     4921 acggtaagag gaattggcac tgaaccaggg tagtccggca gagtaacggt gacatcacga
     4981 gcagaaacat cgcagccgcc agtaggcacc accacatcat tattggcgta aatattccac
     5041 acaaactgaa aatcatcgct gttatagttg ttggtctgtc gcaaaataag cacggcaatt
     5101 aatgagccag ctttaatcgc caccccgccc gcactgctca caggcgtcaa ataaagcgcc
     5161 accggccacg gcttatccgt tctcgaatta taaacaacgc gcggcgtttc gctggtggta
     5221 ggaaatggat cgctactgcc actatatttt acggtcccgg aaaaattaga taacacgctg
     5281 ccataagccg agcctcgttg cagtgtgaca tagtctgtaa tggtttccgg ataatcgtta
     5341 tggcaaaaga tttgcgtcga aagatccacg accaggtttt gccccacatt cacggcgggc
     5401 gcaaggttta cataaacatt ggcgctgcca ccgccaatag ggatagcggt accattggcg
     5461 gttttacagg cgaatgacca ggcatttacc gaccagccca tcagcagtac agcaaacagg
     5521 gtaataactc gtttcattac aatcatctct tcgggttcaa ctgtaggtat aggtgatgct
     5581 aatcactgcc tgaatggttc cctgagtggc tccgccattt accgtcaatg ctctgacctg
     5641 taacgggaag tgcgctgatt gtgaggaatc atccacctga actgttttgg ttgcgccagt
     5701 attcaatgtg ttgccactgt catcctgtag ctctaactgg atgttttgcg cggtcccctg
     5761 gtttttataa tatccggtac tgtcggctgc cccgctgaag ctggcggtga ccctcgacgt
     5821 tcccaccgga caattagtca actcaagcgc aacatcatgc caggccgatg ccgccccggc
     5881 agacatcaga ctgaaagaat aaagatcgcc gagatcaacc gtggcattgg tggtggaaac
     5941 cgtacacggt ttggcgacga ccttaccgtt caccgtgatg gtgacatcgg ctgcctgtat
     6001 cgtcgcactt gcgagcgcca atattgccgc caataaatac ccacgtttgc gccatttcat
     6061 gagcatctcc agttactgat attcaagagt gaaggtagcc gtggcattga tatgccccgc
     6121 agtgacaggc acctgtgtcg ccattagccg ggcgtaaaaa ttcaacgtat ttggtttacc
     6181 cggcgtcagg gtcgtccacg aaatcgcgga cgatggagca ttaaggggta tttgattttg
     6241 ctgctcattc agaagctgta ttcccagtcc cgaagccgct gacaccgtat tttcaagtgc
     6301 aagcagattg gcattgtggc tatctgcaac gccggtaaac ccaactttta cggcagaaac
     6361 ggcattacca cagggtgaca gcaaaatacg aaatggaaca acaggagtcg tcgcgccaat
     6421 gttgttaaat tgcttcgccg cgttttccat cagatcaaca gtaaaattgg ttgattcagc
     6481 ggccacacta cagccgttat ctctgacata gccgcggata gtaatcgtgc tatccgcagc
     6541 caaagcgtga ctcaccgcca gccacaaaaa agcgcacaga agataaaaag gtttgtttct
     6601 catcacgccc ccttaacgac attcagctga tagctgggtt aataactgct gctgactctc
     6661 tggtggcagt tgataattgg cgacacagtg agcattttcc tcttctcccc atttcacctg
     6721 aacttttccc gctaaaggca ttccgctgag gtaaacctga ccattatccg caacaatgcc
     6781 gctactctgg ctactctctg atgtcaccat cgccccaaac ggcagcggct tattattgtg
     6841 ggttagcgtc atgagcagtt ttatcccaac gcgcgcttta aactctgctc gcacgatcgc
     6901 cccacgagtg ggaacaacgt tagcgaccgc gttatctaaa tcgacgttat cagccagggt
     6961 attggtatcc agcgccactc tgttttccct atattcagtg gcataaggca gcacggcata
     7021 accgcgccag tcggtacgca cccccgtctg gttttcgact tttgcatctt ttgcgccagg
     7081 cgctttaaca agcaccaccg tatcgtttaa cggctgcccc agcgttacgc cattggcatg
     7141 agccaatacc ccaccgctga ctccgtaata gagctgctta atatcatcgc tatggctgta
     7201 accgatattg gcattgccgt aaccaccgcg ataattcagc gtggcgtagc ctgtgcttcc
     7261 gctattacca tcgcctcccc cggcatagcc ggtttgcacg ctatagctga ggttgttgtc
     7321 ttccagcaac gtaccgtata caccagccag attggtcatc cgaccgttga gatcgtgtga
     7381 catgctgtag ctggcactgg catgtcgcca ctgagattta ctgtcagaac gcagccagtg
     7441 gctgaaagga atattgacgt taagcgctaa catctgatca cgtccttttt gccaggcgtt
     7501 tttcgtcagg ctatagctga gcgtccagtt gatatcttcg aacgcagtat ttaatccagc
     7561 ctggaattgc tcatcgacat tactcgttcc ccaataagtt tgatggctac cactcaaata
     7621 cagtgttgat gtgcgcccga gttgctgagt aacggtgagc tgtaatttcc cgcgtttgtt
     7681 ataagcgagg ttgtaatagt cggtgaattt cggcttaacc tgaataactc catcctgtgt
     7741 ttcgatgttg tagccattca ttcgactgta tgttgtatca gcgaaattaa aatatccgct
     7801 ggtcgaataa cggtaaccca ctaactgaat attcgtgcct gattcattga gcgatttgtt
     7861 atagagaaaa cgcaccgatt gtccgtcatg ctgactgtca tcgggaagtg tggaattagc
     7921 ctgcgtcata tccacagaca gagcgcccag tgcccccatg tttttcccga taccgaaatt
     7981 aaaagcacga taacgatccg ccagttgcgt tccaccatat attgtccagc cagccggaag
     8041 gccgtggagt aatgtactct ggaaaaagcg gggtttttcc tgctgcgcat ttccactacg
     8101 gtattctcct gccgtaatgg aataacgagt atgcccttca cgttgcaaaa gcgggactga
     8161 cgaatagggt acggtaaaaa tctgcgtgct gccgtcagcc tctttgatcg ttacctgcaa
     8221 gtcaccacta ttacctgcgg catagatatc gttgatggta aaaggccccg gtggcaccgt
     8281 actattataa atgtcatacc cattttgttt aatagtgacc tgtgcagtac cacgagcaat
     8341 accgtggatc accggggcaa atcctctttg gctatcgggt aacatattgt catctgaggc
     8401 caattgtgcg ccgcgaaagt taataccatc gaaaatatcg ccctgagtat aaccatcacc
     8461 cagcgtcagc cgggaacgta acggtattat gtctcgctca agccaggtat tgatatgctg
     8521 ccatttattt ttgctacctg atgatctgtc gctactgtta taactccagg tggtattgtc
     8581 gcgtaaacgc cacgcaccaa tatttaaccc actctgtagg tttaaatatg cataatggct
     8641 gttaccccca atccgattct gtacactatt tccgctgaaa ttataattga gcaatccggc
     8701 attaataccg ggatcccata actcaggagg aatataacca cgcgcgcgat tactcataaa
     8761 tgcctgaggg atcgtcaggt tcagtcgctg ctgaccaaca tccagatgcg cagtagcgtc
     8821 ctggaccatt gtggttaatg gcacacaggc atcatccgcc agcagattca taccggcgac
     8881 agaagccgta ttcagcccca tactggcgag ttgcgcgcgt gtcaggcagg gaacaatccc
     8941 ttgttcactg tcgcccgtat taaatgtgac atcacgcgtt gccatataac cattattcaa
     9001 atagatatcg acgcgatacg tccctggcgg taattcttgc ccattttcaa aacgcgataa
     9061 atcggccaca gcctggggat catccgctaa aaaacgcggg ttaaaataga gttcggcaga
     9121 tgacaaaggt gcctgtgcgg caaaagcaca ggcaacaaag agccggacaa aaaaaccagc
     9181 caaacgatgc ttacgaatat gcaagcattg tgtgtttcgc tggtaaagtc ttaaattcag
     9241 atatgacatt attcctccgg tttaaagtca cccggcagtc aattcttttt attcaggcga
     9301 aaaattcccc ctgcgttatt ccattacgcc cgtcattttg ggggtaagtg caccataatc
     9361 atttattgtt cggtaagtaa tattgcttcc cgcatcagaa ggcaatttaa ccgtgctttc
     9421 gcccattgga ggcaccaagg cattttcaag aacccgggtt ccggcattca actctgttac
     9481 cgtcaggtaa tagggtgtcg ggttaatcag cgtcagagaa ttcgcgctac gacgaaatct
     9541 taatttttct gcggcctgat cgggtggcaa cgctaattta gccgggcgat agtacagttt
     9601 aatgcggctg ataattgcga gctgtagcgt attctcagtc aattttgatt tatccattga
     9661 cggaatcgct ttaacattca tccagaataa actttcccga tcctgtggca attggttatt
     9721 tgttgcatca agaatacgca aggtattctc tttttttccc ttcatcgcaa acagaggagg
     9781 cgtcacgata aaacgaccat cctttacacc atcggcattt tccacccatg attgaattaa
     9841 ataggtacta ttttcatcat tatttgtcac ggcaagttgc acttgttttt gccctgccgg
     9901 ataaattacg cgagtcgcac ctaaggccac tcccgcttca gcgcgtccgg caaccatcat
     9961 tgccataaac atcaggatac ctgccagcaa gcagaatgtt atttcctgcg atttccttac
    10021 attgacgttt ttattactca ctgtcctgtt cctgttatca cattatctgc tgaacaatta
    10081 ctgataggtt aaagagaacc aggcctgggc attggcgatg ccgccagtaa cccgacgccc
    10141 ggtagcacga tatttggcga tgaaatgtag cgaagtagag cctgagtaaa gccgtttcca
    10201 gtttgctgga ggacgattaa tcggtacgag gtttccttca tcatcaaaca acgctacgcc
    10261 aatattggtg gctatccctg gcccctctcc cacggaaagc acatccggat ttttaccatc
    10321 cgcgacaccg tgaaacgcca cccccacacg ttcactcacc accgtgctac attcccgtaa
    10381 atgaataaca aaaggcaccg gtgcgctatc ttcccctgcc gcatgaaacc ggttactgct
    10441 gatttgcccc atattgaccg tcatttgttt atcaccggct tcaatccggc aagtttccgc
    10501 aataatgacg ccctgaaatt gcatatttcc gccgggcagc gtggtattcc atttatttcc
    10561 ggccagagca aacattggca gcaacgacgc taacagaaat aatctcttcc ttttcatcgt
    10621 tatttttatc gcacaagggt gggcatccct gcccgtaatg acgtccttga acctgggtag
    10681 gttattgata ctgaaccttg aaggttgcat ccgcattagc tgcacccggg gttgcctcgc
    10741 cgattgcata ataacgcgcc tggaacggaa tggtgttggt accgttattc agggttgttt
    10801 gtgcactaaa tgtcgcaccg tccagcgtca gagcattgcc tgttctgtcc aggatctgca
    10861 cgccaacgtt tgttgcacta cctgcagcag aactctgcag agccagtaca tcagtacgcg
    10921 tagcatcaat tgccgtacct aagaaggcaa cagcggcttt tgtggcaaca gtggtatcgc
    10981 aatcattcaa ctgaatgtta aaaccaacgg cagagctggt tgctccagcc tgcttcaggc
    11041 tagcggtacg aacctgtccc aactgaacgg tttgatcaac agagcctgca tcaactgcgc
    11101 aagcggcgtt aacaacttcc cctttaaagt gaacggtccc accatttacc gtcgtagtat
    11161 cggccagagc cgctgcggaa ctgagggaca gagccgacag aacaacgatt gccagagttt
    11221 taattttcat gctgctttcc tttaaaaaaa ctatttctaa atcgacatgg gcagtcgttc
    11281 tgtacacttt gttttgtcaa cgagtttgac tgccaacact gcacagtttt cccccaaaag
    11341 atgaaacatt tggggccatt ttgactcata gaggaaagca tcgcgcacaa actttttcag
    11401 tttatttgtt ggcttaatat tctattgtta tctttattta tagattttta tattgcatga
    11461 ggtggttttt ggagagaaga atgaggaaga tgcgtcgagc cacagaaa
//
LOCUS       DAZKHF020000138        11395 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2201S31241
            SAMN43267800-rid25106413.denovo.139, whole genome shotgun sequence.
ACCESSION   DAZKHF020000138 DAZKHF020000000
VERSION     DAZKHF020000138.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267800
            Sequence Read Archive: SRR30307263
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 11395)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11395)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 11395)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350581.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350581.2
            Genome Coverage       :: 151x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:21:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,652
            CDSs (total)                      :: 5,557
            Genes (coding)                    :: 5,320
            CDSs (with protein)               :: 5,320
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 237
            CDSs (without protein)            :: 237
            Pseudo Genes (ambiguous residues) :: 0 of 237
            Pseudo Genes (frameshifted)       :: 102 of 237
            Pseudo Genes (incomplete)         :: 130 of 237
            Pseudo Genes (internal stop)      :: 52 of 237
            Pseudo Genes (multiple problems)  :: 41 of 237
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11395
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267800-rid25106413.denovo.139"
                     /serotype="O 26:H11"
                     /isolate="2201S31241"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-01"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            276..1664
                     /gene="narU"
                     /locus_tag="ACHOSC_RS15085"
                     /old_locus_tag="pgaptmp_003017"
     CDS             276..1664
                     /gene="narU"
                     /locus_tag="ACHOSC_RS15085"
                     /old_locus_tag="pgaptmp_003017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate/nitrite transporter NarU"
                     /protein_id="HGU4548916.1"
                     /translation="MALQNEKNSRYLLRDWKPENPAFWENKGKHIARRNLWISVSCLL
                     LAFCVWMLFSAVTVNLNKIGFNFTTDQLFLLTALPSVSGALLRVPYSFMVPIFGGRRW
                     TVFSTAILIIPCVWLGIAVQNPNTPFGIFIVIALLCGFAGANFASSMGNISFFFPKAK
                     QGSALGINGGLGNLGVSVMQLVAPLVIFVPVFAFLGVNGVPQADGSVMSLANAAWIWV
                     PLLAIATIAAWSGMNDIASSRASIADQLPVLQRLHLWLLSLLYLATFGSFIGFSAGFA
                     MLAKTQFPDVNILRLAFFGPFIGAIARSVGGAISDKFGGVRVTLINFIFMAIFSALLF
                     LTLPGTGSGNFIAFYAVFMGLFLTAGLGSGSTFQMIAVIFRQITIYRVKMKGGSDEQA
                     QKEAVTETAAALGFISAIGAVGGFFIPQAFGMSLNMTGSPVGAMKVFLIFYIVCVLLT
                     WLVYGRRKFSQK"
     gene            1746..5486
                     /gene="narZ"
                     /locus_tag="ACHOSC_RS15090"
                     /old_locus_tag="pgaptmp_003018"
     CDS             1746..5486
                     /gene="narZ"
                     /locus_tag="ACHOSC_RS15090"
                     /old_locus_tag="pgaptmp_003018"
                     /EC_number="1.7.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase Z subunit alpha"
                     /protein_id="HGU4548917.1"
                     /translation="MSKLLDRFRYFKQKGETFADGHGQVMHSNRDWEDSYRQRWQFDK
                     IVRSTHGVNCTGSCSWKIYVKNGLVTWEIQQTDYPRTRPDLPNHEPRGCPRGASYSWY
                     LYSANRLKYPLIRKRLIELWREALKQHSDPVLAWASIMNDPQKCLSYKQVRGRGGFIR
                     SNWQELNQLIAAANVWTIKTYGPDRVAGFSPIPAMSMVSYAAGTRYLSLLGGTCLSFY
                     DWYCDLPPASPMTWGEQTDVPESADWYNSSYIIAWGSNVPQTRTPDAHFFTEVRYKGT
                     KTIAITPDYSEVAKLCDQWLAPKQGTDSALAMAMGHVILKEFHLDNPSDYFINYCRRY
                     SDMPMLVMLEPRDDGSYVPGRMIRASDLVDGLGESNNPQWKTVAVNTAGELVVPNGSI
                     GFRWGEKGKWNLESIAAGTETELSLTLLGQHDAVAGVAFPYFGGIENPHFRSVKHNPV
                     LVRQLPVKNLTLVDGNTCPVVSVYDLVLANYGLDRGLEDENSAKDYAEIKPYTPAWGE
                     QITGVPRQYIETIAREFADTAHKTHGRSMIILGAGVNHWYHMDMNYRGMINMLIFCGC
                     VGQSGGGWAHYVGQEKLRPQTGWLPLAFALDWNRPPRQMNSTSFFYNHSSQWRYEKVS
                     AQELLSPLADASKYSGHLIDFNVRAERMGWLPSAPQLGRNPLGIKAEADKAGLSPTEF
                     TAQALKSGDLRMACEQPDSGSNHPRNLFVWRSNLLGSSGKGHEYMQKYLLGTESGIQG
                     EELGASDGIKPEEVEWQTAAIEGKLDLLVTLDFRMSSTCLFSDIVLPTATWYEKDDMN
                     TSDMHPFIHPLSAAVDPAWESRSDWEIYKGIAKAFSQVCVGHLGKETDVVLQPLLHDS
                     PAELSQPCEVLDWRKGECDLIPGKTAPNIVAVERDYPATYERFTSLGPLMDKLGNGGK
                     GISWNTQDEIDFLGKLNYTKRDGPAQGRPLIDTAIDASEVILALAPETNGHVAVKAWQ
                     ALGEITGREHTHLALHKEDEKIRFRDIQAQPRKIISSPTWSGLESDHVSYNAGYTNVH
                     ELIPWRTLSGRQQLYQDHPWMRAFGESLVAYRPPIDTRSVSEMRQIPPNGFPEKALNF
                     LTPHQKWGIHSTYSENLLMLTLSRGGPIVWISETDARELTIVDNDWVEVFNANGALTA
                     RAVVSQRVPPGMTMMYHAQERIMNIPGSEVTGMRGGIHNSVTRVCPKPTHMIGGYAQL
                     AWGFNYYGTVGSNRDEFIMIRKMKNVNWLDDEGRDQVQEAKK"
     gene            5483..7027
                     /gene="narH"
                     /locus_tag="ACHOSC_RS15095"
                     /old_locus_tag="pgaptmp_003019"
     CDS             5483..7027
                     /gene="narH"
                     /locus_tag="ACHOSC_RS15095"
                     /old_locus_tag="pgaptmp_003019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009325 - nitrate reductase complex
                     [Evidence IEA];
                     GO_function: GO:0008940 - nitrate reductase activity
                     [Evidence IEA];
                     GO_process: GO:0042126 - nitrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase subunit beta"
                     /protein_id="HGU4548918.1"
                     /translation="MKIRSQVGMVLNLDKCIGCHTCSVTCKNVWTGREGMEYAWFNNV
                     ETKPGIGYPKNWEDQEEWQGGWVRDVNGKIRPRLGSKMGVITKIFANPVVPQIDDYYE
                     PFTFDYEHLHSAPEGKHIPTARPRSLIDGKRMDKVIWGPNWEELLGGEFEKRARDRNF
                     EAMQKEMYGQFENTFMMYLPRLCEHCLNPSCVATCPSGAIYKREEDGIVLIDQDKCRG
                     WRLCISGCPYKKIYFNWKSGKSEKCIFCYPRIESGQPTVCSETCVGRIRYLGVLLYDA
                     DRIEEAASTEREVDLYERQCEVFLDPHDPSVIEEALKQGIPQNVIEAAQRSPVYKMAM
                     DWKLALPLHPEYRTLPMVWYVPPLSPIQSYADAGGLPKSEGVLPAIESLRIPVQYLAN
                     MLSAGDTGPVLRALKRMMAMRHYMRSQTVEGVTDTRAIDEVGLSVAQVEEMYRYLAIA
                     NYEDRFVIPTSHREMAGDAFAERNGCGFTFGDGCHGSDSKFNLFNSSRIDAINITEVR
                     DKAEGE"
     gene            7027..7722
                     /gene="narW"
                     /locus_tag="ACHOSC_RS15100"
                     /old_locus_tag="pgaptmp_003020"
     CDS             7027..7722
                     /gene="narW"
                     /locus_tag="ACHOSC_RS15100"
                     /old_locus_tag="pgaptmp_003020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0051131 - chaperone-mediated protein
                     complex assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase molybdenum cofactor assembly
                     chaperone"
                     /protein_id="HGU4548919.1"
                     /translation="MQILKVIGLLMEYPDELLWECKEDALALIRSDAPMLTDFTHNLL
                     NAPLLDKQAEWCEVFDRGRTTSLLLFEHVHAESRDRGQAMVDLLAEYEKVGLQLDCRE
                     LPDYLPLYLEYLSVLPDDQAKEGLLNVAPILALLGGRLKQREAPWYALFDALLQLAGS
                     SLSSDSVTKQVNSEERDDTRQALDAVWEEEQVKFIEDNATACDSSPLNQYQRRFSQDV
                     APQYVDISAGGGK"
     gene            7719..8399
                     /gene="narI"
                     /locus_tag="ACHOSC_RS15105"
                     /old_locus_tag="pgaptmp_003021"
     CDS             7719..8399
                     /gene="narI"
                     /locus_tag="ACHOSC_RS15105"
                     /old_locus_tag="pgaptmp_003021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009325 - nitrate reductase complex
                     [Evidence IEA];
                     GO_function: GO:0008940 - nitrate reductase activity
                     [Evidence IEA];
                     GO_process: GO:0019645 - anaerobic electron transport
                     chain [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="respiratory nitrate reductase subunit gamma"
                     /protein_id="HGU4548920.1"
                     /translation="MIQYLNVFFYDIYPYICATVFFLGSWLRYDYGQYTWRASSSQML
                     DKREMVIWSNLFHIGILGIFFGHLFGMLTPHWMYAWFLPVAAKQLMAMVLGGICGVLT
                     LIGGAGLLWRRLTNQRVRATSTTPDIIIMSILLIQCLLGLSTIPFSAQYPDGSEMMKL
                     VGWAQSIVTFRGGSSEMLNGVAFVFRLHLVLGMTIFLLFPFTRLVHVWSAPFEYFTRR
                     YQIVRSRR"
     gene            8478..9371
                     /locus_tag="ACHOSC_RS15110"
                     /old_locus_tag="pgaptmp_003022"
     CDS             8478..9371
                     /locus_tag="ACHOSC_RS15110"
                     /old_locus_tag="pgaptmp_003022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PhzF family isomerase"
                     /protein_id="HGU4548921.1"
                     /translation="MKPQVYHVDAFTSQPFRGNSAGVVFPADNLSEAQMQLIARELGH
                     SETAFLLHSDDSDVRIRYFTPTVEVPICGHATVAAHYVRAKVLGLGNCTIWQTSLAGK
                     HRVTIEKHNDDYRISLEQGTPGFEPPLEGETREAIINALHLTEDDILPGLPIQVATTG
                     HSKVMIPLKPEVDIDALSPDLAALSAISKQIGCNGFFPFQIRPGKNETDGRMFSPAIG
                     IVEDPVTGNANGPMGAWLVHHNVLPHDGNVLCVKGHQGRALGRDGMIEVTVTIRDNQP
                     EKVTISGTAVILFHAEWAIEL"
     gene            complement(9467..10312)
                     /gene="nhoA"
                     /locus_tag="ACHOSC_RS15115"
                     /old_locus_tag="pgaptmp_003023"
     CDS             complement(9467..10312)
                     /gene="nhoA"
                     /locus_tag="ACHOSC_RS15115"
                     /old_locus_tag="pgaptmp_003023"
                     /EC_number="2.3.1.118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016407 - acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-hydroxyarylamine O-acetyltransferase"
                     /protein_id="HGU4548922.1"
                     /translation="MTPILNHYFARINWSGAAAVNIDTLRALHLKHNCTIPFENLDVL
                     LPREIQLDDQSLEEKLVIARRGGYCFEQNGVFERVLRELEFNVRSLLGRVVLSNPPAL
                     PPRTHRLLLVELEEEKWIADVGFGGQTLTAPIRLVSDLVQTTPHGDYRLLQEGDDWVL
                     QFNHHQHWQSMYRFDLCEQQQSDYVMGNFWSAHWPQSHFRHHLLMCRHLPDGGKLTLT
                     NFHFTHYENGHAVEQRNLPDVASLYAVMQEQFGLGVDDAKHGFTVDELALVMAAFDTH
                     PEAGK"
     gene            10485..11054
                     /locus_tag="ACHOSC_RS15120"
                     /old_locus_tag="pgaptmp_003024"
     CDS             10485..11054
                     /locus_tag="ACHOSC_RS15120"
                     /old_locus_tag="pgaptmp_003024"
                     /EC_number="1.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415979.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016646 - oxidoreductase activity, acting
                     on the CH-NH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavin reductase family protein"
                     /protein_id="HGU4548923.1"
                     /translation="MSRFIPIELHHASRLLNHGPTVMITSFDEQSQRRNIMAAAWSMP
                     VEFEPPRVAIVVDKSTWTRELIERNGKFGIVIPGVAATNWTWAVGSVSGREEDKFNCY
                     GIPVVKGPVLGLPLVEEKCLAWMECRLLPATSAQQKYDTLFGEVVSAAADARVFVEGR
                     WQFDDDKLNTLHHLGAGTFVTSGKRVTAG"
     gene            complement(11058..11285)
                     /gene="pptA"
                     /locus_tag="ACHOSC_RS15125"
                     /old_locus_tag="pgaptmp_003025"
                     /pseudo
     CDS             complement(11058..11285)
                     /gene="pptA"
                     /locus_tag="ACHOSC_RS15125"
                     /old_locus_tag="pgaptmp_003025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310091.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tautomerase PptA"
BASE COUNT         2685 a         2962 c         3014 g         2734 t
ORIGIN      
        1 tagttaatat gtaacccttt agattcacga tctgaagggt tgaaacatga catacgttca
       61 aagattaacc cccgtttgaa ggttagcttg ttagttacat ttagtaacac atatctggct
      121 acttaatccc cattctgccc ccgcggttac gctttcttat ctattctttc aatcagcact
      181 tcacccaatt catccttaat tctaataact ggctattgcg ccttacctcc tgttttgtct
      241 tttttcatta ccaatgtgtg catgtgagga acaatatggc actgcaaaat gagaaaaata
      301 gtcgttatct tttacgcgac tggaaaccag aaaatccggc cttctgggaa aataaaggaa
      361 aacatattgc tcgaagaaac ctctggatat cagtcagttg tctacttctt gccttctgtg
      421 tctggatgct atttagcgca gttaccgtta atctcaataa aatcggtttt aattttacta
      481 ccgatcaact ctttttatta accgcattac cctccgtttc tggcgcatta ttgcgtgttc
      541 cctactcctt tatggtgcct atattcggtg gacgccgatg gacggttttt agtactgcaa
      601 tcctgattat tccttgcgtc tggctcggaa ttgccgtgca aaatccgaat actccttttg
      661 ggatatttat cgttatcgct ttgctatgcg gttttgcagg tgcaaacttt gcttcgagca
      721 tgggcaatat cagtttcttc tttccaaaag ccaaacaagg gagcgctctt gggattaatg
      781 gcggattagg aaacttaggt gtaagtgtaa tgcagctggt tgcaccgctg gtcatttttg
      841 tacctgtatt tgcctttctc ggcgtcaatg gcgtaccgca ggccgacggt tcggtgatgt
      901 cgctggcgaa tgccgcatgg atttgggtac cgctactggc gattgccacg atcgccgcat
      961 ggtcagggat gaatgatatc gccagttcac gcgcctcaat tgccgaccag ctccctgtct
     1021 tacaacgcct gcatctctgg ctgctgagcc tgctttacct tgccaccttc ggttcgttta
     1081 tcggtttttc tgcgggtttt gccatgctgg caaaaaccca gttcccggat gtgaatattc
     1141 tgcgcctggc gttctttggc ccatttatcg gtgccatcgc gcggtcggtt ggtggtgcta
     1201 tttccgataa gttcggcggc gtgcgggtga cgttgatcaa ctttattttt atggcgattt
     1261 tcagtgccct gctgttcctt accttaccgg gcacaggctc cggtaatttc atcgcctttt
     1321 acgccgtatt tatggggctg tttctgactg cgggtctggg aagtggttct actttccaga
     1381 tgatcgccgt catctttcgc cagataacca tttatcgggt aaagatgaaa ggcggtagtg
     1441 atgagcaagc tcagaaagaa gccgtcaccg aaacggcggc ggctctgggc tttatctcag
     1501 ccattggcgc agtgggcggc ttttttattc cgcaggcgtt tggcatgtcg ctcaatatga
     1561 ccggctctcc ggtcggcgcg atgaaagtgt ttttaatctt ctacatcgtt tgtgtgctgc
     1621 tgacctggct ggtttatggt cggcggaagt tcagccaaaa ataagagatc gatgcctgat
     1681 gtacaaccgt gtcgcatccg acaaggcatc agatcccaat tcattcctgg agcaggagtc
     1741 atgtcatgag taaacttttg gatcgctttc gctacttcaa acaaaagggc gaaacctttg
     1801 ccgatggtca cggacaggtg atgcatagca accgcgactg ggaggacagc tatcgccagc
     1861 gttggcagtt cgacaaaatc gtgcgttcca cccacggtgt taactgtaca ggctcctgta
     1921 gctggaaaat ctacgttaaa aatggtctgg tgacctggga aatccaacag accgactacc
     1981 cgcgcactcg ccctgacctg cccaatcatg aacctcgcgg ctgcccgcgt ggcgcaagtt
     2041 actcctggta tctttacagc gctaaccgcc tgaaataccc gctcattcgt aaacgactga
     2101 ttgaactgtg gcgcgaagcc ctcaagcaac acagcgatcc ggtactggcg tgggcatcga
     2161 ttatgaacga tccgcaaaag tgcctgagct acaaacaagt gcgtgggcgc ggcgggttta
     2221 tccgctccaa ctggcaggaa ctaaaccagc tgattgccgc cgctaacgtc tggaccatca
     2281 aaacctacgg cccggatcgc gttgccggtt tctcgccgat cccggcgatg tcgatggttt
     2341 cttacgccgc cggaacgcgt tatctgtcgc tgcttggcgg cacctgttta agtttctacg
     2401 actggtattg cgacctgccg cccgcctcgc cgatgacctg gggcgagcaa accgacgtac
     2461 cggaatctgc cgactggtat aactccagct acatcatcgc ctgggggtct aacgtaccgc
     2521 agacacgtac gccggacgcc cacttcttta ccgaagtacg ctacaaaggc actaaaacca
     2581 tcgccattac ccctgactac tctgaagtgg ccaaattgtg cgaccagtgg ctggcaccga
     2641 aacaaggcac tgatagcgcc ctggcgatgg caatgggcca tgtgatttta aaagagtttc
     2701 atctcgataa tcccagcgac tactttatca actactgccg ccgctacagc gacatgccga
     2761 tgctggtaat gctggagcct cgcgacgatg gtagctacgt tcccgggcgc atgatccgcg
     2821 catctgacct ggtggatgga ctgggcgaaa gcaacaatcc gcagtggaaa accgtagcag
     2881 ttaataccgc aggtgaattg gtagtgccga acggttcgat tggtttccgc tggggagaaa
     2941 aaggcaaatg gaatctggaa tccattgccg ccggtacgga aaccgaattg tcgttaaccc
     3001 tgctcggtca acatgacgct gttgcaggcg tggccttccc ctactttggc ggcattgaaa
     3061 atccgcattt tcgcagcgta aaacacaatc cggtgctggt gcgccaattg cccgttaaaa
     3121 acctgacgtt agtcgatggc aacacctgtc cggtggtcag cgtttatgat ttggtactgg
     3181 cgaattacgg cctcgatcgc gggctggaag atgaaaacag tgcgaaagat tacgctgaaa
     3241 tcaaaccgta caccccagcc tggggtgagc aaattaccgg cgtgccgcgc cagtatattg
     3301 aaaccatcgc ccgtgaattt gccgataccg cccataaaac gcatgggcgc tcgatgatta
     3361 tcctcggcgc aggtgttaac cactggtatc acatggacat gaactaccgc gggatgatca
     3421 atatgctgat cttctgcggt tgtgtcgggc aaagcggtgg cggctgggca cactatgtcg
     3481 gccaggaaaa actgcgccca caaaccggct ggttgccgct ggcctttgcg ctcgactgga
     3541 accgaccacc acgccagatg aacagtacct cgtttttcta caatcattcc agccaatggc
     3601 gctatgaaaa agtctctgct caggagttac tttcaccgct cgccgatgcc agtaagtaca
     3661 gcggtcatct gattgatttc aacgttcgcg ccgaacgtat gggctggcta ccttctgcgc
     3721 cgcagctggg gcgtaacccg ctcgggatta aagctgaagc cgacaaggcc ggattatccc
     3781 ccacagaatt taccgcccag gcgctgaaat cgggcgattt acgtatggcc tgcgaacaac
     3841 cagatagcgg cagcaatcat ccgcgtaatt tgtttgtctg gcgctctaac ctgcttggct
     3901 cctccggcaa aggccacgag tatatgcaga agtatctgct ggggaccgaa agcgggattc
     3961 agggcgagga actcggtgcc agcgacggga tcaaaccgga agaagtcgag tggcaaactg
     4021 cggcgattga aggcaagctc gacctgctgg tgacgctcga cttccgcatg tccagtacct
     4081 gcctgttctc cgatatcgtt ctgcccaccg ccacctggta cgaaaaagac gatatgaaca
     4141 cctcggatat gcatccgttt attcatccgc tttctgcggc ggtcgatccg gcgtgggaat
     4201 cacgcagcga ctgggaaatc tacaaaggta tcgccaaagc attttcgcaa gtgtgcgtgg
     4261 gtcatcttgg caaagaaacc gacgtggtat tacaacccct actgcatgac tctccggcag
     4321 agctctcaca gccgtgtgaa gtgctcgact ggcgcaaagg cgaatgcgat ctgattccag
     4381 gcaaaaccgc gccgaatatt gtggcggtgg agcgcgacta ccctgctacg tatgaacgct
     4441 ttacctcgct cgggccattg atggacaaac ttggcaatgg cggtaaaggg atttcgtgga
     4501 atacgcagga tgaaatcgat ttcctcggca aactcaatta caccaagcgt gatggcccgg
     4561 cacaggggcg tccgctgatt gacaccgcca ttgacgcttc agaagtgatt ctggcactgg
     4621 caccggaaac caacggtcat gttgcagtca aagcgtggca ggcgctgggc gagatcaccg
     4681 ggcgcgaaca tacccatctg gcgctgcaca aagaggacga gaagattcgc tttcgcgata
     4741 ttcaggcgca gccgcgtaaa attatctcca gccccacctg gtccggtctg gaaagcgatc
     4801 acgtatccta taacgcggga tacaccaacg ttcatgagtt aattccgtgg cgcacgctgt
     4861 cgggacgcca gcagctctat caggatcatc cgtggatgcg tgcttttggt gaaagcctgg
     4921 tggcttatcg cccgcctatc gacacccgta gcgtcagtga gatgcgccag ataccgccaa
     4981 acggcttccc ggaaaaagca cttaacttcc tgacgccgca ccagaaatgg ggcattcact
     5041 caacctacag tgaaaacctg ctaatgctga cgctctctcg cggtggaccg attgtctgga
     5101 tcagcgaaac agatgcccga gaactgacca ttgtcgataa cgactgggtg gaagtattca
     5161 acgccaacgg cgcgctgact gcccgcgcgg tggtcagcca acgtgtaccg ccgggcatga
     5221 ccatgatgta tcacgcccag gaacgcatta tgaatattcc tggttcggaa gtaactggca
     5281 tgcgcggcgg cattcataac tcggtcaccc gcgtttgccc gaaaccaacg catatgattg
     5341 gcggttacgc gcagctggcc tggggcttta actactacgg caccgtcgga tcgaaccgcg
     5401 atgagttcat catgatccgc aagatgaaga acgttaactg gctggatgat gaaggtcgcg
     5461 atcaggtaca ggaggcgaaa aaatgaaaat ccgttcacaa gtcggcatgg tgcttaacct
     5521 cgacaaatgt atcggctgcc atacctgttc ggtgacctgt aaaaacgtct ggaccgggcg
     5581 cgaaggcatg gagtacgcat ggtttaacaa cgtcgaaacc aaaccgggca ttggttatcc
     5641 gaaaaactgg gaagatcagg aagagtggca aggcggctgg gtccgcgatg tgaatggcaa
     5701 gatccgtccg cgtctgggca gcaagatggg cgtaataacc aaaatcttcg ccaacccggt
     5761 ggtgccgcag attgatgatt actacgaacc tttcaccttc gactacgaac atttgcatag
     5821 cgcaccggaa ggcaaacata tccctactgc tcgcccgcgt tcactgattg acggcaaacg
     5881 gatggacaaa gtgatctggg gcccaaactg ggaagaactg ctgggcggcg agtttgaaaa
     5941 acgtgcccgc gaccgcaact tcgaggccat gcaaaaggag atgtacgggc agtttgaaaa
     6001 caccttcatg atgtacctgc cgcgcctgtg cgaacactgc ctcaatccca gttgcgtggc
     6061 gacctgccca agcggcgcta tctacaaacg cgaagaagac ggcattgtgc tgattgatca
     6121 ggataaatgc cgtggctggc gtttgtgcat tagcggttgt ccgtacaaaa aaatctactt
     6181 caactggaaa agcggcaagt cagaaaaatg tatcttctgt tatccgcgaa ttgagtcagg
     6241 ccaacccacc gtgtgctcag aaacctgcgt gggtcgcatc cggtatctgg gcgtgctgct
     6301 ttacgacgcc gaccgcattg aggaagcggc gagcaccgag cgcgaagttg acctctatga
     6361 acgtcagtgc gaagtattcc tcgatccaca cgatccctca gtgatcgagg aagccctgaa
     6421 acaaggtatt ccacaaaacg tgattgaagc tgcccagcgt tcgcctgtct acaaaatggc
     6481 gatggactgg aaactggcgc taccgctgca ccctgaatat cgcaccctgc caatggtctg
     6541 gtacgttcct ccgctgtcgc cgattcagtc ctacgcagat gcgggcggtt tgccgaaaag
     6601 cgaaggcgtg ctgcccgcca tcgaaagcct gcgtattccg gtgcaatatc tcgccaatat
     6661 gttgagtgcc ggcgataccg gtccggtact gcgggcgctg aaacggatga tggcgatgcg
     6721 ccactatatg cgttcacaaa ccgtggaagg cgttactgat actcgtgcca tcgacgaagt
     6781 aggcctgagc gtcgcccagg tcgaagagat gtatcgttac ctcgccattg ccaactatga
     6841 agatcgtttt gtcatcccga cgagccatcg ggaaatggcg ggcgatgcct tcgcagaacg
     6901 caacggctgc ggttttacct ttggcgacgg ttgccacggt tcggacagta aattcaacct
     6961 gttcaacagt agccgtatcg atgccatcaa catcaccgaa gtgcgcgaca aagcggaggg
     7021 cgaataatgc agatcctcaa agtgatcggc ctgttgatgg agtatccgga cgagctgttg
     7081 tgggaatgca aggaggacgc gctggcgttg atccgcagcg acgcgccgat gcttacggat
     7141 ttcacccaca acctgcttaa cgcgccgctg ctggataaac aggccgaatg gtgcgaagtg
     7201 tttgaccgtg gacgtaccac atcgctgctg ctgttcgaac atgttcatgc cgagtcccgc
     7261 gatcgcggcc aggcaatggt ggacctgctg gcggagtatg aaaaggtcgg cctgcagctg
     7321 gattgtcggg aactgcccga ttatctaccg ctgtatctgg agtatttaag cgtactgcct
     7381 gacgatcagg cgaaagaagg attgctcaat gttgcgccga tcctcgccct gcttggcggt
     7441 cgcttaaaac aacgcgaggc accgtggtac gcgttgtttg atgctctgct gcaactggcg
     7501 ggaagctccc tttcaagtga cagtgtcaca aaacaagtaa acagcgaaga gcgcgatgac
     7561 acccgccagg cgcttgatgc agtgtgggaa gaggaacagg tgaagtttat tgaagataac
     7621 gccacggcgt gtgacagctc gccgcttaat caatatcagc gacgctttag ccaggatgtc
     7681 gcgccgcaat atgtcgacat cagtgcggga ggtgggaaat gattcagtat ctgaacgtct
     7741 ttttttacga tatctacccg tacatttgtg cgacggtgtt tttcctcggc agctggctgc
     7801 gctacgacta cgggcagtac acctggcgcg cctcctcaag tcaaatgctc gataaacgcg
     7861 agatggtgat atggtcgaat ctgttccata tcggcatttt ggggattttc ttcgggcact
     7921 tgttcggcat gttaacgccg cactggatgt acgcgtggtt tttgccagtg gcagcgaaac
     7981 aactaatggc gatggtgctc ggcggtattt gcggtgtgtt gacattgatt ggcggcgcag
     8041 gactgctgtg gcgcaggctg acaaatcagc gcgtgcgtgc aacttccact acgccggata
     8101 ttatcatcat gagcattttg ctgatccagt gtctgctggg gctaagtacc ataccgtttt
     8161 cagcacagta tcctgatggt agcgaaatga tgaagctggt gggttgggcg caaagcattg
     8221 tcactttccg tggtggatcg tcagaaatgc ttaacggcgt agcgttcgtt ttccgcctgc
     8281 atctggtatt gggaatgacc atcttcctgc tcttcccgtt caccagattg gtgcatgtgt
     8341 ggagcgcgcc gtttgagtac tttactcgtc ggtatcagat tgtgcgttcg cggcgataat
     8401 ttcaccctgg ccggatagcc cttcgctctc cggccacttg caggctatga tagttctcca
     8461 gtcacaggag aacgttaatg aaaccgcagg tgtaccacgt cgatgctttt acctctcaac
     8521 catttcgcgg caattctgcc ggtgttgttt tccccgccga taatcttagc gaagcgcaaa
     8581 tgcagcttat cgcccgcgag ttaggccatt cggaaactgc ttttctgctg cacagcgacg
     8641 acagcgatgt gcgcatccgc tactttacgc caacggttga agtgccgatt tgcggtcacg
     8701 cgacagtagc tgcgcactat gtacgtgcga aggtgttagg tttaggaaat tgcacgatct
     8761 ggcaaacatc gctggcagga aaacatcgcg tgactatcga aaagcacaac gatgattatc
     8821 gtatttcgct ggaacaaggt acgccgggct ttgagccacc gctggaaggt gaaacacgtg
     8881 aggcgattat caacgcgctt catctcactg aagacgatat tttgccgggc ttgccaattc
     8941 aggtcgcaac aacaggacac tcaaaagtga tgatcccgtt gaaaccggaa gtggatatcg
     9001 acgccctttc gcctgatctt gctgcgctga gcgctatcag caaacagatt ggctgcaatg
     9061 gtttcttccc gttccagatc cgcccaggca aaaacgaaac cgatggtcgt atgttctcgc
     9121 ctgcgattgg tattgtggaa gatccggtga ctggcaatgc caatggcccg atgggtgcat
     9181 ggttggtaca tcacaacgta ttgccccacg atggcaacgt gttatgtgtt aaaggccatc
     9241 aggggcgcgc attggggcgt gacggcatga ttgaagtgac ggtgacaatt cgtgataacc
     9301 aaccggaaaa agtcaccatt tcaggcacag cggtgatttt gtttcatgcc gaatgggcaa
     9361 ttgaactctg aaaccgttgc ctgatgcgct tcgcctgtca ggtctacaac cgagctaatg
     9421 tatgccggat aaggcgttta cgccgcatcc ggcaacctga cataaattat tttcccgcct
     9481 ccgggtgcgt atcaaacgcc gccatcacca gcgctaactc atccacggta aagccatgtt
     9541 tcgcatcatc cacgcccaga ccaaactgtt cttgcatcac agcatataat gacgccacat
     9601 ccggtagatt tcgctgctcc accgcgtgcc cattttcata atgggtaaaa tgaaaattgg
     9661 tcagcgttag cttgccaccg tccggcaaat ggcggcacat cagcaaatga tggcgaaaat
     9721 gcgactgcgg ccagtgcgcc gaccagaaat tgcccatcac ataatcgctt tgttgctgct
     9781 cgcagagatc aaaacggtac atcgactgcc agtgctgatg atgattaaac tgcaacaccc
     9841 aatcatcacc ctcctgcaac aaccgataat ctccgtgtgg cgtggtctgc acgagatcgg
     9901 aaactaaacg aatcggcgcg gttagcgtct gcccaccgaa accgacatca gcaatccatt
     9961 tttcctcttc cagttccacc aacagcaaac gatgggtgcg cggcggtaat gctggcggat
    10021 ttgataacac tacgcgccct aacaagctgc gaacgttaaa ctccagctcg cgtaacaccc
    10081 gctcaaacac gccattctgc tcaaaacagt aaccgccacg acgggctatc accagtttct
    10141 cttccagcga ttgatcatca agctgtattt ccctcggcag caaaacgtcg aggttttcaa
    10201 acggaatagt gcaattgtgt ttcaggtgca atgcacgaag cgtatcaata ttgaccgcag
    10261 cagctcccga ccagttaata cgggcaaaat agtgattcag aatgggcgtc atcaggcgtt
    10321 tcccttagtg gttttctcaa cttataacct ggaatagacg ccgcaccggc tattttgtgc
    10381 gatcgcggac atgcatcacg tttgcaaatc gccaatcccg ttaatttcgt tacttattgc
    10441 gttacggact acgcttaaaa aaaccttctt cgcgaggcga taccatgagc cgattcatcc
    10501 ccatagaatt acaccatgcc agccgtctgt taaatcatgg cccaactgtc atgatcacca
    10561 gtttcgatga gcaatcccag cggcgtaaca ttatggcagc agcctggtca atgccggtgg
    10621 agtttgaacc gccacgcgtg gcgattgtgg tagataaatc gacatggacc agggagttga
    10681 ttgaacgtaa cggtaaattt ggcatcgtta tcccgggcgt tgcagcaact aactggacgt
    10741 gggcagtggg aagcgtgtcg ggtcgtgaag aagataaatt taattgctat ggcattccgg
    10801 tggtgaaagg tccggtgctt ggtttgccac ttgttgaaga gaaatgcctg gcgtggatgg
    10861 agtgccgcct gctacctgcg acttctgcgc aacaaaaata cgacacgctg tttggtgagg
    10921 tggtatcagc agcggcagac gcacgggtat ttgtcgaagg ccgctggcag tttgatgatg
    10981 ataagctcaa tacgttgcat catttaggtg ctggcacgtt tgttaccagc ggcaagcgag
    11041 ttacggcggg ttaagcatta catgctgtaa ccgggtttct ttatcaaagc ctccatttgg
    11101 ggcgcgattt cggcatccca gatagcttgc caagattctg gttgaatctg ctgtagagca
    11161 atgcttatcg agctatcttt actgttcaga tggcgaataa taacgtcggt aatatctgca
    11221 gcaagtgctg ctttttattg ttcgtccagt tcacgcggaa aacatttaat gtcgatgtgc
    11281 ggcatgattg tgctcctttt gaaagatgtc agtaaaatta acacacattg tgaatgtcgt
    11341 gtgtccggta atgcgttttt acgacaataa tgccagggca agattacgaa agcct
//
LOCUS       DAZKHF020000139        11393 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2201S31241
            SAMN43267800-rid25106413.denovo.140, whole genome shotgun sequence.
ACCESSION   DAZKHF020000139 DAZKHF020000000
VERSION     DAZKHF020000139.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267800
            Sequence Read Archive: SRR30307263
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 11393)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11393)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 11393)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350581.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350581.2
            Genome Coverage       :: 151x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:21:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,652
            CDSs (total)                      :: 5,557
            Genes (coding)                    :: 5,320
            CDSs (with protein)               :: 5,320
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 237
            CDSs (without protein)            :: 237
            Pseudo Genes (ambiguous residues) :: 0 of 237
            Pseudo Genes (frameshifted)       :: 102 of 237
            Pseudo Genes (incomplete)         :: 130 of 237
            Pseudo Genes (internal stop)      :: 52 of 237
            Pseudo Genes (multiple problems)  :: 41 of 237
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11393
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267800-rid25106413.denovo.140"
                     /serotype="O 26:H11"
                     /isolate="2201S31241"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-01"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            303..1169
                     /gene="folD"
                     /locus_tag="ACHOSC_RS15130"
                     /old_locus_tag="pgaptmp_003026"
     CDS             303..1169
                     /gene="folD"
                     /locus_tag="ACHOSC_RS15130"
                     /old_locus_tag="pgaptmp_003026"
                     /EC_number="1.5.1.5"
                     /EC_number="3.5.4.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004488 - methylenetetrahydrofolate
                     dehydrogenase (NADP+) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional methylenetetrahydrofolate
                     dehydrogenase/methenyltetrahydrofolate cyclohydrolase
                     FolD"
                     /protein_id="HGU4548924.1"
                     /translation="MAAKIIDGKTIAQQVRSEVAQKVQARIAAGLRAPGLAVVLVGSN
                     PASQIYVASKRKACEEVGFVSRSYDLPETTSEAELLELIDALNADNTIDGILVQLPLP
                     AGIDNVKVLERIHPDKDVDGFHPYNVGRLCQRAPRLRPCTPRGIVTLLERYNIDTFGL
                     NAVVIGASNIVGRPMSMELLLAGCTTTVTHRFTKNLRHHVENADLLIVAVGKPGFIPG
                     DWIKEGAIVIDVGINRLENGKVVGDVVFEDAAKRASYITPVPGGVGPMTVATLIENTL
                     QACVEYHDPQDE"
     gene            1171..1383
                     /gene="ybcJ"
                     /locus_tag="ACHOSC_RS15135"
                     /old_locus_tag="pgaptmp_003027"
     CDS             1171..1383
                     /gene="ybcJ"
                     /locus_tag="ACHOSC_RS15135"
                     /old_locus_tag="pgaptmp_003027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308617.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated protein YbcJ"
                     /protein_id="HGU4548925.1"
                     /translation="MATFSLGKHPHVELCDLLKLEGWSESGAQAKIAIAEGQVKVDGA
                     VETRKRCKIVAGQTVSFAGHSVQVVA"
     gene            1491..2012
                     /locus_tag="ACHOSC_RS15140"
                     /old_locus_tag="pgaptmp_003028"
     CDS             1491..2012
                     /locus_tag="ACHOSC_RS15140"
                     /old_locus_tag="pgaptmp_003028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HGU4548926.1"
                     /translation="MPTVITHAAVPLCIGLGLGSKVIPPRLLFAGIILAMLPDADVLS
                     FKFGVAYGNVFGHREFTHSLVFAFVVPLLCVLIGRRWFRAGLIRCWLFLTVSLLSHSL
                     LDSVTTGGKGVGWLWPWSDERFFAPWQVIKVAPFALSRYTTPYGHQVIISELMWVWLP
                     GMVLMGMLWWRRR"
     gene            complement(2048..3433)
                     /gene="cysS"
                     /locus_tag="ACHOSC_RS15145"
                     /old_locus_tag="pgaptmp_003029"
     CDS             complement(2048..3433)
                     /gene="cysS"
                     /locus_tag="ACHOSC_RS15145"
                     /old_locus_tag="pgaptmp_003029"
                     /EC_number="6.1.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005125243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004817 - cysteine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006423 - cysteinyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine--tRNA ligase"
                     /protein_id="HGU4548927.1"
                     /translation="MLKIFNTLTRQKEEFKPIHAGEVGMYVCGITVYDLCHIGHGRTF
                     VAFDVVARYLRFLGYKLKYVRNITDIDDKIIKRANENGESFVALVDRMIAEMHKDFDA
                     LNILRPDMEPRATHHIAEIIELTEQLIAKGHAYVADNGDVMFDVPTDPTYGVLSRQDL
                     DQLQAGARVDVVDDKRNPMDFVLWKMSKEGEPSWPSPWGAGRPGWHIECSAMNCKQLG
                     NHFDIHGGGSDLMFPHHENEIAQSTCAHDGQYVNYWMHSGMVMVDREKMSKSLGNFFT
                     VRDVLKYYDAETVRYFLMSGHYRSQLNYSEENLKQARAALERLYIALRGTDKTVAPAG
                     GEAFEARFIEAMDDDFNTPEAYSVLFDMAREVNRLKAEDMAAANAMASHLRKLSAVLG
                     LLEQEPEAFLQSGAQADDSEVAEIEALIQQRLDARKAKDWAAADAARDRLNEMGIVLE
                     DGPQGTTWRRK"
     gene            3607..4101
                     /gene="ppiB"
                     /locus_tag="ACHOSC_RS15150"
                     /old_locus_tag="pgaptmp_003030"
     CDS             3607..4101
                     /gene="ppiB"
                     /locus_tag="ACHOSC_RS15150"
                     /old_locus_tag="pgaptmp_003030"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017465360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase B"
                     /protein_id="HGU4548928.1"
                     /translation="MVTFHTNHGDIVIKTFDDKAPETVKNFLDYCREGFYNNTIFHRV
                     INGFMIQGGGFEPGMKQKATKEPIKNEANNGLKNTRGTLAMARTQAPHSATAQFFINV
                     VDNDFLNFSGESLQGWGYCVFAEVVEGMDVVDKIKGVATGRSGMHQDVPKEDVIIESV
                     TVSE"
     gene            4104..4826
                     /gene="lpxH"
                     /locus_tag="ACHOSC_RS15155"
                     /old_locus_tag="pgaptmp_003031"
     CDS             4104..4826
                     /gene="lpxH"
                     /locus_tag="ACHOSC_RS15155"
                     /old_locus_tag="pgaptmp_003031"
                     /EC_number="3.6.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008758 - UDP-2,3-diacylglucosamine
                     hydrolase activity [Evidence IEA];
                     GO_function: GO:0016462 - pyrophosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-2,3-diacylglucosamine diphosphatase"
                     /protein_id="HGU4548929.1"
                     /translation="MATLFIADLHLCVEEPAITAGFLRFLAGEARKADALYILGDLFE
                     AWIGDDDPNPLHRQMAAAIKAVSDSGVPCYFIHGNRDFLLGKRFARESGMTLLPEEKV
                     LELYGRRVLIMHGDTLCTDDAGYQAFRAKVHKPWLQMLFLALPLFVRKRIAARMRANS
                     KEANSSKSLAIMDVNQNAVVSAMEKHQVQWLIHGHTHRPAVHELIANQQPAFRVVLGA
                     WHTEGSMVKVTADDVELIHFPF"
     gene            4944..5453
                     /gene="purE"
                     /locus_tag="ACHOSC_RS15160"
                     /old_locus_tag="pgaptmp_003032"
     CDS             4944..5453
                     /gene="purE"
                     /locus_tag="ACHOSC_RS15160"
                     /old_locus_tag="pgaptmp_003032"
                     /EC_number="4.1.1.21"
                     /EC_number="5.4.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009320 - phosphoribosylaminoimidazole
                     carboxylase complex [Evidence IEA];
                     GO_function: GO:0004638 - phosphoribosylaminoimidazole
                     carboxylase activity [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide mutase"
                     /protein_id="HGU4548930.1"
                     /translation="MSSRNNPARVAIVMGSKSDWATMQFAAEIFEILNVPHHVEVVSA
                     HRTPDKLFSFAESAEENGYQVIIAGAGGAAHLPGMIAAKTLVPVLGVPVQSAALSGVD
                     SLYSIVQMPRGIPVGTLAIGKAGAANAALLAAQILATHDKELHQRLNDWRKAQTDEVL
                     ENPDPRGAA"
     gene            5450..6517
                     /gene="purK"
                     /locus_tag="ACHOSC_RS15165"
                     /old_locus_tag="pgaptmp_003033"
     CDS             5450..6517
                     /gene="purK"
                     /locus_tag="ACHOSC_RS15165"
                     /old_locus_tag="pgaptmp_003033"
                     /EC_number="6.3.4.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706400.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004638 - phosphoribosylaminoimidazole
                     carboxylase activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide
                     synthase"
                     /protein_id="HGU4548931.1"
                     /translation="MKQVCVLGNGQLGRMLRQAGEPLGIAVWPVGLDAEPAAVPFQQS
                     VITAEIERWPETALTRELARHPAFVNRDVFPIIADRLTQKQLFDKLHLPTAPWQLLAD
                     RSEWPAVFERLGELAIVKRRTGGYDGRGQWRLRANETEQLPAECYGECIVEQGINFSG
                     EVSLVGARGFDGSTVFYPLTHNLHQDGILRTSVAFPQANAQQQAQAEEMLSAIMQELG
                     YVGVMAMECFVTPQGLLINELAPRVHNSGHWTQNGASISQFELHLRAITDLPLPQPVV
                     NSPSVMINLIGSDVNYDWLKLPLVHLHWYDKEVRPGRKVGHLNLTDSDTSRLTATLEA
                     LIPLLPPEYASGVMWAQSKFS"
     gene            complement(6654..7547)
                     /locus_tag="ACHOSC_RS15170"
                     /old_locus_tag="pgaptmp_003034"
     CDS             complement(6654..7547)
                     /locus_tag="ACHOSC_RS15170"
                     /old_locus_tag="pgaptmp_003034"
                     /EC_number="2.7.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008804 - carbamate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006525 - arginine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbamate kinase"
                     /protein_id="HGU4548932.1"
                     /translation="MKTLVVALGGNALLQRGEALTAENQYRNIASAVPALARLARSYR
                     LAIVHGNGPQVGLLALQNLAWKEVDPYPLDVLVAESQGMIGYMLAQSLSAQPQMPPVT
                     TVLTRIEVSPDDPAFLQPEKFIGPVYQPEEQKALEAAYGWQMKRDGKYLRRVVASPQP
                     RKILDSEAIELLLKEGHVVICSGGGGVPVTEDGAGSEAVIDKDLAAALLAEQINADGL
                     VILTDADAVYENWGTPQQRAIRHATPDELAPFTKADGSMGPKVTAVSGYVRSRGKPAW
                     IGALSRIEETLAGEAGTCISL"
     gene            complement(7544..8359)
                     /locus_tag="ACHOSC_RS15175"
                     /old_locus_tag="pgaptmp_003035"
     CDS             complement(7544..8359)
                     /locus_tag="ACHOSC_RS15175"
                     /old_locus_tag="pgaptmp_003035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2877 domain-containing protein"
                     /protein_id="HGU4548933.1"
                     /translation="MTIIHPLLASRSAPNYRQSWRLAGVWRRAINLMTESGELLTLHR
                     QGSGFGPGGWVLRRAQFDALCGGLCGNERPQVVAQGIRLGRFTVKQPQRYCLLRITPP
                     SHPQPLAAAWMQRAEETGLFGPLAMAASDPLPAELRQFRHCFQAALNGVKTDWRHWLG
                     KGPGLTPSHDDTLSGMLLAAWYYGALDARAGRPFFACSDNLQLVTTAVSVSYLRYAAQ
                     GYFASPLLHFVHALSCPKRTAVAIDSLLALGHTSGADTLLGFWLGQQLLQGKP"
     gene            complement(8370..9629)
                     /locus_tag="ACHOSC_RS15180"
                     /old_locus_tag="pgaptmp_003036"
     CDS             complement(8370..9629)
                     /locus_tag="ACHOSC_RS15180"
                     /old_locus_tag="pgaptmp_003036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009029994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1116 domain-containing protein"
                     /protein_id="HGU4548934.1"
                     /translation="MFTSVAQANAAVIEQIRRARPHWLDVQPASSLISELNEGKTLLH
                     AGPPMRWQEMTGPMKGACVGACLFEGWAKDEAQALAILEQGEVNFIPCHHVNAVGPMG
                     GITSASMPMLVVENVTDGNRAYCNLNEGIGKVMRFGAYGEDVLTRHRWMRDVLMPVLS
                     AALGRMERGIDLTAMMAQGITMGDEFHQRNIASSALLMRALAPQIARLDHDKQHIAEV
                     MDFLSVTDQFFLNLAMAYCKAAMDAGAMIRAGSIVTAMTRNGNMFGIRVSGLGERWFT
                     APVNTPQGLFFTGFSQEQANPDMGDSAITETFGIGGAAMIAAPGVTRFVGAGGMEAAR
                     AVSEEMAEIYLERNMQLQIPGWDFQGACLGLDIRRVVETGITPLINTGIAHKEAGIGQ
                     IGAGTVRAPLACFEQALEALAESMGIG"
     gene            complement(9639..11306)
                     /locus_tag="ACHOSC_RS15185"
                     /old_locus_tag="pgaptmp_003037"
     CDS             complement(9639..11306)
                     /locus_tag="ACHOSC_RS15185"
                     /old_locus_tag="pgaptmp_003037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA synthetase FdrA"
                     /protein_id="HGU4548935.1"
                     /translation="MIHAFIKKGCFQDSVSLMIISRKLSESENVDDVSVMMGTPANKA
                     LLDTTGFWHDDFNNATPNDICVAIRSEAADAGIAQAIMQQLEEALKQLAQGSGSSQAL
                     TQVRRWDSACQKLPDANLALISVAGEYAAELTNQALDRNLNVMMFSDNVTLEDEIQLK
                     TRAREKGLLVMGPDCGTSMIAGTPLAFANVMPEGNIGVIGASGTGIQELCSQIALAGE
                     GITHAIGLGGRDLSREVGGISALTALEMLSADEKSEVLAFVSKPPAEAVRLKIVNAMK
                     ATGKPTVALFLGYTPAVARDENVWFASSLDEAARLACLLSRVTARRNAIAPVSSGFIC
                     GLYTGGTLAAEAAGLLAGHLGVEADDTHHHGMMLDADGHQIIDLGDDFYTVGRPHPMI
                     DPALRNQLIADLGAKPQVRVLLLDVVIGFGATADPAASLVSAWQKACAARSDSQPLYA
                     IATVTGTERDPQCRSQQIATLEDAGIAVVSSLPEATLLAAALIHPLSSATQQHTPSLL
                     ENVAVINIGLRSFALELQSASKPVVHYQWSPVAGGNKKLARLLERLQ"
BASE COUNT         2530 a         3248 c         3018 g         2597 t
ORIGIN      
        1 ttcaacacaa atatagacct gaagcggtaa attaccaggc tgaaaattct ttttatattg
       61 tcaggtattt cttaaattat cttaatcctt aaacaaggaa ataaatcagt tccagattta
      121 caacgccatc atggacgaaa aatgaagctt tcaatctcag cgacctcagc gacggtgcgc
      181 ctcaccttcg caagaggtcg cttcacgcga taaatctgaa acgaaacctg acagcgcgcc
      241 ccgcttctga caaaataggc gcatcccctt cgacctacgt aacagatgga atcctctctc
      301 tgatggcagc aaagattatt gacggtaaaa cgattgcgca gcaggtgcgc tctgaagttg
      361 ctcaaaaagt tcaggcgcgt attgcagccg gactgcgggc accaggactg gccgttgtgc
      421 tggtgggtag taaccctgca tcgcaaattt atgtcgcaag caaacgcaag gcttgtgaag
      481 aagtcgggtt cgtctcccgc tcttatgacc tcccggaaac caccagcgaa gcggagctgt
      541 tggagcttat cgatgcgctg aatgccgaca acaccatcga tggcattctg gttcaactgc
      601 cgttaccggc gggtattgat aacgtcaaag tgctggaacg tattcatccg gacaaagacg
      661 tggacggttt ccatccttat aacgtcggtc gtctgtgcca gcgcgcgccg cgtctgcgtc
      721 cctgcacccc gcgcggtatc gtcacgctgc ttgagcgtta caacattgat accttcggcc
      781 tcaacgccgt ggtgattggc gcatcgaata tcgtcggtcg tccaatgagc atggaactgt
      841 tgctcgcagg ttgcaccact accgtgactc accgtttcac taaaaatctg cgtcaccacg
      901 tagaaaatgc cgatctgttg atcgttgccg tcggcaagcc gggctttatt cctggtgact
      961 ggatcaaaga aggcgcaatt gtgattgatg tcggcatcaa ccgtctggaa aatggcaaag
     1021 ttgtgggcga cgtcgtgttt gaagacgcgg ctaaacgcgc ctcatacatt acgcctgttc
     1081 cgggcggcgt tggcccgatg acggttgcca cgctgattga aaacacgcta caggcgtgcg
     1141 ttgaatatca tgatccacag gatgagtaac atggcgacat tttctttagg taaacatccg
     1201 cacgttgagc tgtgcgactt gctgaaactg gaaggctgga gcgaaagcgg cgcgcaggcg
     1261 aaaatcgcga ttgccgaagg ccaggtgaaa gtcgacggtg cggttgaaac gcgcaaacgc
     1321 tgcaaaatcg tcgccggtca gacagtgagt tttgcaggtc acagcgtaca ggttgttgcc
     1381 tgatcccttt cccggcctgt atcaacaggc cggttctctt ttcagacttt tgccctctac
     1441 gccccgttca tccatgctga tagcatcgac attttgccga agcgaccatc atgccaaccg
     1501 ttattacgca cgccgccgtt cccctatgca ttggtttagg gttgggttcg aaagtcatcc
     1561 ctccgcgtct gttatttgcc ggaatcatcc tggcgatgct gcccgacgcc gacgtattgt
     1621 cgtttaaatt tggcgttgct tacggcaatg tttttggtca tcgcgagttt acccattcgc
     1681 tggtgtttgc gtttgttgtc ccgctgttat gtgtgctgat tggacgccga tggttcaggg
     1741 ctgggctgat tcgctgctgg ctgtttttaa ccgtctcgtt gctatcgcac agcttgctgg
     1801 attcggtaac cactggcggt aagggcgttg gctggctgtg gccgtggtca gatgaacgct
     1861 ttttcgctcc ctggcaggtg attaaagtcg cgccgtttgc attgtctcgt tacaccacgc
     1921 cgtacgggca tcaggtgatt atttctgaat tgatgtgggt gtggttgccg gggatggtgc
     1981 ttatgggaat gttgtggtgg cgcaggcgat aaccggatgc gaaaactcgc atccggcaac
     2041 agcgcaatta cttacgacgc caggtggtcc cttgcgggcc atcttccagc acgatcccca
     2101 tctcgttaag acggtcacgc gccgcatctg ccgccgccca gtctttcgct ttacgggcat
     2161 ccagacgctg ttgaattaac gcttcaatct cagccacttc gctgtcgtct gcctgcgcac
     2221 cgctttgcag gaacgcttcc ggttcttgct ccagcaggcc caatacagcg gaaagtttac
     2281 gcaggtgaga tgccattgca ttcgccgctg ccatatcttc tgctttcaga cggttcactt
     2341 cacgcgccat atcaaacagc acggaatagg cttccggggt gttgaaatcg tcgtccatcg
     2401 cttcaataaa gcgcgcttca aacgcttcgc caccggcagg cgcaacggtt ttatctgtgc
     2461 cgcgcagcgc aatgtagagg cgctccagcg ccgcacgcgc ctgcttcagg ttctcttcgc
     2521 tgtagttcag ttggctgcga tagtggcccg acatcaggaa gtaacgcacg gtttccgcgt
     2581 cgtagtattt cagcacatcg cgcacggtaa agaagttacc cagcgatttg gacatcttct
     2641 cgcggtcaac catcaccatc cccgagtgca tccagtagtt cacatactgg ccatcatggg
     2701 cacaggtgga ctgcgcgatt tcgttctcgt ggtgcgggaa catcaggtct gaaccgccgc
     2761 cgtggatatc aaagtggtta cccagctgct tgcagttcat tgccgaacat tcaatgtgcc
     2821 agcctggacg gcccgcgccc cacggagacg gccagctcgg ttcgccctct ttcgacatct
     2881 tccacagaac gaagtccatc gggttgcgtt tgtcgtcgac cacgtcaacg cgcgcacctg
     2941 cctgcagctg gtcgagatcc tgacgcgaca gcacgccata tgtcggatcg gtcgggacgt
     3001 cgaacatcac gtcgccgttg tccgccacat aagcgtgacc tttggcgatc agttgttcag
     3061 tgagttcaat aatttctgcg atatggtgcg tcgcgcgcgg ctccatatcc gggcgcagaa
     3121 tgttcaaagc atcaaaatct ttgtgcattt cggcgatcat gcgatccacc agcgccacaa
     3181 agctttcgcc attttcattg gcgcgtttga tgattttgtc gtcgatatcg gtaatgttgc
     3241 gcacatactt cagtttatag ccgaggaaac gcagatagcg cgcaaccacg tcaaaagcaa
     3301 caaaggtacg cccgtgaccg atatgacaga gatcgtaaac ggtgattcca cacacataca
     3361 tgccgacttc cccggcgtga ataggcttaa attcctcttt ttggcgtgtc agagtattga
     3421 agatttttag catcgaagat tccgtttaga catgtgtggg taattgagtt gcgtataata
     3481 cccatatttc ccgcgcgaat cagcatacat tgcgtgatga tccgatcgtg cggttatgct
     3541 ataacaccac cctatatatg acccgaactg ggttgaagca ccaatcaaac ggaacaggat
     3601 gcaaaaatgg ttactttcca caccaatcac ggcgatattg tcatcaaaac ttttgacgat
     3661 aaagcacctg aaacagttaa aaacttcctg gactactgcc gcgaaggttt ttacaacaac
     3721 accattttcc accgtgttat caacggcttt atgattcagg gcggcggttt tgaaccgggc
     3781 atgaaacaaa aagccaccaa agaaccgatc aaaaacgaag ccaacaacgg tctgaaaaat
     3841 acccgtggta cgctggcaat ggcccgtact caggctccgc actctgcaac tgcacagttc
     3901 ttcatcaacg tggttgataa cgacttcctg aacttctctg gcgaaagcct gcaaggttgg
     3961 ggctactgcg tgtttgctga agtggttgaa ggcatggacg tggtagacaa aatcaaaggt
     4021 gttgcaaccg gtcgtagcgg tatgcaccag gacgtgccaa aagaagacgt tatcattgaa
     4081 agcgtgaccg ttagcgagta atcgtggcga cactctttat tgcagatctt catctctgcg
     4141 tggaagaacc ggcgatcacc gccggttttc tgcgtttttt agcgggggaa gcccgcaagg
     4201 ccgacgcgct gtatattctt ggcgatctgt ttgaagcatg gattggcgac gacgatccca
     4261 acccactcca tcgccagatg gcggcagcga tcaaagcggt gtccgattcc ggcgttccct
     4321 gttatttcat tcatggcaac cgtgattttc tgctcggcaa acgctttgcc cgtgaaagcg
     4381 gcatgacatt attgccggaa gaaaaggtgc tcgaacttta tggtcgccgg gtgttgatta
     4441 tgcatggcga cacgctgtgc accgatgacg cgggttatca ggcttttcgc gccaaagtcc
     4501 acaaaccctg gctgcaaatg ctattcctcg ccctgccgtt gtttgtgcgc aaacgcattg
     4561 ccgcgcgaat gcgagcgaac agtaaagaag ccaacagcag taaatcgctg gcgatcatgg
     4621 acgttaacca aaacgcggta gtcagtgcga tggaaaaaca tcaggtgcaa tggctgatcc
     4681 acggacatac ccatcgcccg gcggtacatg aacttatcgc caaccagcaa cctgcttttc
     4741 gcgtggtact cggtgcctgg catacggaag gttcgatggt gaaagtcacg gcggatgacg
     4801 ttgagctgat tcattttccg ttttaaaaaa cccgcaactt tgctgatttc acagccacgc
     4861 aaccgttttc cttgctctct ttccgtgcta ttctctgtgc cctctaaagc cgagagttgt
     4921 gcaccacagg agttttaaga cgcatgtctt cccgcaataa tccggcgcgt gtcgccatcg
     4981 tgatggggtc caaaagcgac tgggctacca tgcagttcgc cgccgaaatc ttcgaaatcc
     5041 tgaatgtccc gcaccacgtt gaagtggttt ctgctcaccg cacccccgat aagctgttca
     5101 gcttcgccga aagcgccgaa gagaacggtt atcaggtgat tattgcgggc gcaggcggcg
     5161 cagcgcacct gcctggtatg attgccgcca aaacgctggt gccagtgctg ggcgtgccag
     5221 tacagagcgc cgcactgagc ggtgtcgata gcctctactc catcgtacaa atgccgcgcg
     5281 gcattccggt gggtacgctg gcgattggta aagctggcgc ggcaaacgcg gcgttactgg
     5341 cagcacaaat tcttgcgact catgataaag aactgcacca gcgtctgaat gactggcgca
     5401 aagcccagac cgacgaagtg ctggaaaacc cggacccgcg aggtgcggca tgaaacaggt
     5461 ttgcgtcctc ggtaacgggc agttaggccg tatgctgcgt caggcaggtg aaccgttagg
     5521 cattgctgtc tggccggtcg ggctggacgc tgaaccggcg gcggtgcctt ttcaacaaag
     5581 cgtgattacc gctgagatcg aacgctggcc ggaaaccgca ttaacccgcg agctggcgcg
     5641 tcatccggcc tttgtgaacc gcgatgtgtt cccgattatt gccgaccgtc tgactcagaa
     5701 gcagcttttc gataagctcc acctgccgac cgcaccgtgg cagttacttg ccgatcgcag
     5761 cgagtggcct gcggtgtttg agcgtttagg tgaactggcg attgttaagc gtcgcactgg
     5821 tggctatgac ggtcgcggtc aatggcgttt acgtgccaat gaaaccgaac agttaccggc
     5881 agagtgttac ggcgaatgta ttgtcgagca gggcattaac ttctctggtg aagtgtcgct
     5941 ggttggcgca cgcggatttg atggcagcac cgtgttttat ccgctgacgc ataacctgca
     6001 tcaggacggt attttgcgca ccagcgtcgc ttttccgcag gccaatgcgc agcagcaagc
     6061 gcaagccgaa gagatgctgt cggcgattat gcaggagctg ggctatgtgg gcgtgatggc
     6121 gatggagtgt tttgtcaccc cgcaaggtct gctgatcaac gaactggcac cgcgtgtgca
     6181 taacagcggt cactggacac aaaacggtgc cagcatcagc cagtttgagc tgcatctgcg
     6241 ggcgattacc gatctgccgt taccgcaacc ggtagtgaat agtccgtcgg tgatgatcaa
     6301 cctgattggt agcgatgtga attatgactg gctgaaactg ccgctggtgc atctgcactg
     6361 gtacgacaaa gaagtccgtc cggggcgtaa agtggggcat ctgaatttga ccgacagcga
     6421 cacatcgcgt ctgaccgcga cgctggaagc cttgatcccg ctgctgccgc cggagtatgc
     6481 cagcggcgtg atgtgggcgc agagtaagtt cagttaagca ggtgctcatt tgattgccgg
     6541 atgcggcgta aacgccttat ccggcttacc gctccggtac ccattgtagg cctgataaga
     6601 cgcgtcaagc gtcgcatcag gcatggtgca ccgattgccg gatgctctca tcactacagc
     6661 gaaatacagg tccccgcttc gcccgccagc gtctcttcaa ttcgcgataa cgccccaatc
     6721 cacgcgggtt taccacggct tctgacataa ccacttaccg ccgttacctt cggccccatc
     6781 gaaccatcgg ctttggtaaa tggcgctaac tcatccggtg tggcatggcg aatggcgcgt
     6841 tgctgcggcg ttccccagtt ttcataaacc gcgtcagcat cggtgaggat caccagtcca
     6901 tcggcattaa tctgctcggc gagcaacgca gcggcgagat ctttatcaat caccgcttca
     6961 ctccctgccc catcttccgt cacaggcaca ccgccgccgc cactgcaaat caccacatgc
     7021 ccctctttga gcaacaactc gatggcttcg ctgtcgagaa ttttacgcgg ttgcggagac
     7081 gccaccaccc ggcgcaaata tttaccatca cgtttcatct gccagccgta agccgcttcc
     7141 agtgcctttt gttcttctgg ctgataaacc ggaccaataa atttctctgg ctgcaaaaac
     7201 gccggatcat caggcgaaac ctcaatgcgc gtcagcaccg tcgtgacagg cggcatctgc
     7261 ggctgtgcgc tcaaactctg cgccagcata tagccaatca tcccctggct ttccgccacc
     7321 agcacatcca gcggatacgg atctacctct ttccacgcca gattctgcaa tgccagcagc
     7381 cccacctgcg gcccattgcc gtgaacaatc gccagccgat aagaacgggc caggcgcgcc
     7441 agcgcgggta cagcactggc gatattgcga tattgatttt ctgccgtcag cgcctcaccg
     7501 cgctggagta aggcgttgcc cccaagagct acaaccagtg ttttcatggt tttccttgta
     7561 ataattgttg gccaagccag aaccccagca gcgtatctgc ccctgacgta tgccccagcg
     7621 ccagcagcga atcaatcgca acagcggtac gcttcgggca actcagagca tggacaaagt
     7681 gcaggagtgg cgaggcgaaa tatccttgcg cggcataacg taaataactg acgctcaccg
     7741 ctgtggtaac gagttgaaga ttgtcggaac aggcaaaaaa cgggcgaccg gcgcgcgcat
     7801 ctaaagcgcc ataataccag gccgccagca gcattccgct caacgtgtca tcatgacttg
     7861 gcgttaatcc ggggccttta cccagccagt gccgccagtc ggtcttaacg ccattgagcg
     7921 cggcctgaaa acagtgacga aactggcgta attcagcggg cagcggatcg cttgccgcca
     7981 tcgccagtgg tccgaaaagt ccggtttcct ccgcgcgttg catccatgca gctgcaagcg
     8041 gttgaggatg cgaaggcggc gtaatacgca gcaaacaata acgctgtggc tgtttaaccg
     8101 tgaaacgccc gaggcgaatc ccttgagcca caacctgtgg tcgttcattg ccgcataatc
     8161 caccgcataa cgcatcgaat tgcgcgcggc gaagcaccca tcctccgggg ccgaaaccac
     8221 taccctgacg atgcagcgtt aacagttcgc cgctttccgt catcaggtta atcgcccgtc
     8281 gccacactcc cgctaaccgc caggactggc gataattcgg tgcactacgg ctggcaagca
     8341 gaggatggat gatcgtcatt gcgcgttcct caaccaatcc ccatgctttc agccagtgct
     8401 tccagcgcct gttcaaaaca cgccagcggt gcccgcacag tgcctgcgcc aatctgcccg
     8461 atccccgcct ctttatgggc gataccggta ttgatgagtg gcgtaatgcc ggtttctacc
     8521 acgcgacgaa tatccagccc caggcacgcg ccctggaaat cccagccggg gatctgcaac
     8581 tgcatattgc gctcaaggta aatttccgcc atctcttcag ataccgctct ggccgcttcc
     8641 atgccacccg caccgacaaa gcgcgttacg ccaggcgctg cgatcattgc cgcacctccg
     8701 ataccaaagg tttcggtaat cgcgctatcg cccatatccg ggttcgcctg ctcctgcgag
     8761 aagccggtga aaaacagacc ttgcggagtg tttacaggcg cagtaaacca gcgttcgccc
     8821 agcccgctta cccgaatccc gaacatattg ccgttgcggg tcattgccgt gacgatgctg
     8881 cctgcgcgga tcatcgcgcc agcatccatc gccgccttgc agtaagccat cgcgaggttg
     8941 aggaagaact gatcggtcac gctgaggaaa tccatcactt cggcgatgtg ctgtttatca
     9001 tgatcgaggc gagcaatttg tggggccagc gcacgcatta acagtgcaga ggaagcaata
     9061 ttgcgttgat ggaactcatc gcccatcgta atgccctgcg ccatcatcgc cgtgagatcg
     9121 ataccgcgct ccatgcgccc cagcgccgcg cttaatactg gcattaacac atcgcgcatc
     9181 cagcgatggc gagtcaggac atcttcgccg taagcgccaa aacgcatcac tttgccgata
     9241 ccttcgttga ggttgcagta cgcccggttg ccgtcggtca cgttctcgac caccagcatc
     9301 ggcatactgg cagaagtaat accgcccatt ggaccgacgg cattcacatg gtgacaagga
     9361 atgaagttca cttccccctg ctccagtatt gccagcgcct gcgcttcatc tttcgcccag
     9421 ccttcgaaca gacatgcgcc cacgcacgcc cctttcatgg gtccggtcat ctcctgccag
     9481 cgcattggcg gcccggcgtg aagcagtgtt ttgccctcgt ttagttcgct gataagtgaa
     9541 gaagccggtt gcacatccag ccagtgtgga cgagcacgac gaatttgttc gataaccgca
     9601 gcattggctt gcgccactga tgtaaacatg ggaacccctt attgcaaacg ttctaataaa
     9661 cgagccagtt ttttattgcc gccggcgact ggcgaccatt ggtaatgcac aaccggtttg
     9721 ctggcgcttt gtagctccag cgcaaagctg cgtaatccga tgttaatcac ggcgacgttt
     9781 tccagtaatg acggtgtgtg ttgctgtgtg gcagacgaga gcggatgaat taacgctgcc
     9841 gccagcaagg tggcttccgg tagcgaactc actaccgcga tccccgcatc ttccagcgtg
     9901 gcgatttgct gcgagcggca ttgcgggtca cgttcggtac ctgtcaccgt ggcaatggcg
     9961 tacagcggtt ggctgtctga acgcgcggca caggcttttt gccaggcgct caccagcgag
    10021 gcagcaggat cggcggtcgc accgaagcca atcacgacat caagcagcaa cacgcgcact
    10081 tgcggtttag cgccgagatc ggcaattaac tggttgcgta aggctgggtc gatcatcgga
    10141 tggggacgcc cgacggtgta gaaatcatcg cccagatcaa tgatctggtg accatcggcg
    10201 tccagcatca tgccgtgatg atgggtatcg tcggcttcca cgccaaggtg cccggcaagt
    10261 aatcccgccg cttcggcagc aagcgtaccg ccggtataca aaccgcaaat aaatccgctg
    10321 ctgacaggcg ctattgcgtt acgtcgcgcc gtgacgcgtg aaagcaggca agccaggcgt
    10381 gcggcctcat ccagcgagga ggcaaaccag acattctcgt cgcgggccac cgccggggta
    10441 taacctaaaa acagcgccac cgtcggtttg ccggttgctt tcatggcatt aacaattttc
    10501 agacgcacag cttcggcagg tggttttgaa acaaatgcca gcacttcgct tttctcgtct
    10561 gcactgagca tttccagcgc tgttagcgca ctgatgccgc ccacttcacg gctgaggtcg
    10621 cgcccgccaa ggccaatcgc gtgagtaatt ccctcccctg ccagcgcaat ctgcgaacac
    10681 agctcctgaa tcccggtacc ggaagcgcca atgacgccaa tattgccttc tggcatcacg
    10741 ttagcaaaag ccagcggtgt gccggcaatc atcgacgtac cgcagtccgg ccccatcacc
    10801 agcaagcctt tttcccgcgc gcgggtttta agttggattt catcttccag cgtgacgtta
    10861 tcggagaaca tcatcacgtt gaggttgcga tccagcgcct ggtttgtcag ctccgccgca
    10921 tactcgccag ccactgaaat cagcgccaga ttggcatcgg gtaatttctg acaggcgctg
    10981 tcccagcgac gcacctgcgt caacgcctgg ctgctgcctg acccctgcgc cagttgtttt
    11041 agcgcctctt caagctgctg cataatcgcc tgcgcgatcc ccgcatccgc cgcttcgcta
    11101 cgaattgcca cgcaaatatc gttcggcgtg gcgttattaa aatcgtcatg ccagaaacct
    11161 gtggtatcta ataacgcttt attggcgggc gtacccatca ttacggaaac atcatcaaca
    11221 ttttctgatt cgctgagttt tcgtgaaata atcattaaac tgaccgaatc ctgaaaacac
    11281 ccttttttaa taaaggcgtg gatcataaca actccttaaa aattgcaccc atggcaattg
    11341 cacgagcagt gttaatgtgg cgattacggt atcgatgaca acgagtaaat aag
//
LOCUS       DAZKHF020000140        11389 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2201S31241
            SAMN43267800-rid25106413.denovo.141, whole genome shotgun sequence.
ACCESSION   DAZKHF020000140 DAZKHF020000000
VERSION     DAZKHF020000140.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267800
            Sequence Read Archive: SRR30307263
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 11389)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11389)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 11389)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350581.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350581.2
            Genome Coverage       :: 151x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:21:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,652
            CDSs (total)                      :: 5,557
            Genes (coding)                    :: 5,320
            CDSs (with protein)               :: 5,320
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 237
            CDSs (without protein)            :: 237
            Pseudo Genes (ambiguous residues) :: 0 of 237
            Pseudo Genes (frameshifted)       :: 102 of 237
            Pseudo Genes (incomplete)         :: 130 of 237
            Pseudo Genes (internal stop)      :: 52 of 237
            Pseudo Genes (multiple problems)  :: 41 of 237
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11389
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267800-rid25106413.denovo.141"
                     /serotype="O 26:H11"
                     /isolate="2201S31241"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-01"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            156..773
                     /locus_tag="ACHOSC_RS15190"
                     /old_locus_tag="pgaptmp_003038"
     CDS             156..773
                     /locus_tag="ACHOSC_RS15190"
                     /old_locus_tag="pgaptmp_003038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1287 domain-containing protein"
                     /protein_id="HGU4548936.1"
                     /translation="MKASLALLSLLTAFTSHSLKSPAVPPTVVQIQANTNLAIADGAR
                     QQIGSTLFYDPAYMQLTYPGGDVPQERGVCSDVVIRALRSQKVDLQKLVHEDMAKNFA
                     EYPQKWQLKRPDSNIDHRRVPNLETWFTRHDKTRPISKNPSDYQAGDIVSWRLDNGLA
                     HIGVVSDGFARDGTPLVIHNIGAGAQEEDVLFSWRMVGHYRYFVK"
     gene            complement(800..1705)
                     /gene="yijE"
                     /locus_tag="ACHOSC_RS15195"
                     /old_locus_tag="pgaptmp_003039"
     CDS             complement(800..1705)
                     /gene="yijE"
                     /locus_tag="ACHOSC_RS15195"
                     /old_locus_tag="pgaptmp_003039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418378.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cystine transporter YijE"
                     /protein_id="HGU4548937.1"
                     /translation="MSVAGKSNPLAISGLVVLTLIWSYSWIFMKQVTSYIGAFDFTAL
                     RCIFGALVLFIVLLLRGRGMRPTPFKYTLAIALLQTCGMVGLAQWALVSGGAGKVAIL
                     SYTMPFWVVIFAALFLGERLRRGQYFAILIAAFGLFLVLQPWQLDFSSMKSAMLAILS
                     GVSWGASAIVAKRLYARHPRVDLLSLTSWQMLYAALVMSVVALLVPQREIDWQPTVFW
                     ALAYSAILATALAWSLWLFVLKNLPASIASLSTLAVPVCGVLFSWWLLGENPGAVEGS
                     GIVLIVLALALVSRKKKEAVSVKSI"
     gene            complement(1799..3979)
                     /gene="katG"
                     /locus_tag="ACHOSC_RS15200"
                     /old_locus_tag="pgaptmp_003040"
     CDS             complement(1799..3979)
                     /gene="katG"
                     /locus_tag="ACHOSC_RS15200"
                     /old_locus_tag="pgaptmp_003040"
                     /EC_number="1.11.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004096 - catalase activity [Evidence
                     IEA];
                     GO_function: GO:0004601 - peroxidase activity [Evidence
                     IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="catalase/peroxidase HPI"
                     /protein_id="HGU4548938.1"
                     /translation="MSTSDDIHNTTATGKCPFHQGGHDQSAGAGTTTRDWWPNQLRVD
                     LLNQHSNRSNPLGEDFDYRKEFSKLDYYGLKKDLKALLTESQPWWPADWGSYAGLFIR
                     MAWHGAGTYRSIDGRGGAGRGQQRFAPLNSWPDNVSLDKARRLLWPIKQKYGQKISWA
                     DLFILAGNVALENSGFRTFGFGAGREDVWEPDLDVNWGDEKAWLTHRHPEALAKAPLG
                     ATEMGLIYVNPEGPDHSGEPLSAAAAIRATFGNMGMNDEETVALIAGGHTLGKTHGAG
                     PTSNVGPDPEAAPIEEQGLGWASTYGSGVGADAITSGLEVVWTQTPTQWSNYFFENLF
                     KYEWVQTRSPAGAIQFEAVDAPEIIPDPFDPSKKRKPTMLVTDLTLRFDPEFEKISRR
                     FLNDPQAFNEAFARAWFKLTHRDMGPKSRYIGPEVPKEDLIWQDPLPQPIYNPTEQDI
                     IDLKFAIADSGLSVSELVSVAWASASTFRGGDKRGGANGARLALMPQRDWDVNAAAVR
                     ALPVLEKIQKESGKASLADIIVLAGVVGVEKAASAAGLSIHVPFAPGRVDARQDQTDI
                     EMFELLEPIADGFRNYRARLDVSTTESLLIDKAQQLTLTAPEMTALVGGMRVLGANFD
                     GSKNGVFTDRVGVLSNDFFVNLLDMRYEWKATDESKELFEGRDRETGEVKYTASRADL
                     VFGSNSVLRAVAEVYASSDAHEKFVKDFVAAWVKVMNLDRFDLL"
     gene            complement(4308..5198)
                     /gene="metF"
                     /locus_tag="ACHOSC_RS15205"
                     /old_locus_tag="pgaptmp_003041"
     CDS             complement(4308..5198)
                     /gene="metF"
                     /locus_tag="ACHOSC_RS15205"
                     /old_locus_tag="pgaptmp_003041"
                     /EC_number="1.5.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004489 - methylenetetrahydrofolate
                     reductase (NAD(P)H) activity [Evidence IEA];
                     GO_process: GO:0009086 - methionine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylenetetrahydrofolate reductase"
                     /protein_id="HGU4548939.1"
                     /translation="MSFFHASQRDALNQSLAEVQGQINVSFEFFPPRTSEMEQTLWNS
                     IDRLSSLKPKFVSVTYGANSGERDRTHSIIKGIKDRTGLEAAPHLTCIDATPDELRTI
                     ARDYWNNGIRHIVALRGDLPPGSGKPEMYASDLVTLLKEVADFDISVAAYPEVHPEAK
                     SAQADLLNLKRKVDAGANRAITQFFFDVESYLRFRDRCVSAGIDVEIIPGILPVSNFK
                     QAKKFADMTNVRIPAWMAQMFDGLDDDAETRKLVGANIAMDMVKILSREGVKDFHFYT
                     LNRAEMSYAICHTLGVRPGL"
     gene            5274..5408
                     /locus_tag="ACHOSC_RS15210"
                     /old_locus_tag="pgaptmp_003042"
     CDS             5274..5408
                     /locus_tag="ACHOSC_RS15210"
                     /old_locus_tag="pgaptmp_003042"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4548940.1"
                     /translation="MKEKPKGVNRKMTNDGEEKSGKMKKLQDGKKKKIRYAKQTGLQI
                     "
     gene            complement(5547..7979)
                     /gene="metL"
                     /locus_tag="ACHOSC_RS15215"
                     /old_locus_tag="pgaptmp_003043"
     CDS             complement(5547..7979)
                     /gene="metL"
                     /locus_tag="ACHOSC_RS15215"
                     /old_locus_tag="pgaptmp_003043"
                     /EC_number="1.1.1.3"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000110794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional aspartate kinase/homoserine
                     dehydrogenase II"
                     /protein_id="HGU4548941.1"
                     /translation="MSVIAQAGAKGRQLHKFGGSSLADVKCYLRVAGIMAEYSQPDDM
                     MVVSAAGSTTNQLINWLKLSQTDRLSAHQVQQTLRRYQCDLISGLLPAEEADSLISAF
                     VSDLERLAALLDSGINDAVYAEVVGHGEVWSARLMSAVLNQQGLPAAWLDAREFLRAE
                     RAAQPQVDEGLSYPLLQQLLVQHPGKRLVVTGFISRNNAGETVLLGRNGSDYSATQIG
                     ALAGVSRVTIWSDVAGVYSADPRKVKDACLLPLLRLDEASELARLAAPVLHARTLQPV
                     SGSEIDLQLRCSYTPDQGSTRIERVLASGTGARIVTSHDDVCLIEFQVPVSQDFKLAH
                     KEIDQILKRAQVRPLAVGVHNDRQLLQFCYTSEVADSALKILDEAGLPGELRLRQGLA
                     LVAMVGAGVTRNPLHCHRFWQQLKGQPVEFTWQSDDGISLVAVLRTGPTESLIQGLHQ
                     SVFRAEKRIGLVLFGKGNIGSRWLELFAREQSTLSARTGFEFVLAGVVDSRRSLLSYD
                     GLDASRALAFFNDEAVEQDEESLFLWMRAHPYDDLVVLDVTASQQLADQYLDFASHGF
                     HVISANKLAGASDSNKYRQIHDAFEKTGRHWLYNATVGAGLPINHTVRDLIDSGDTIL
                     SISGIFSGTLSWLFLQFDGSVPFTELVDQAWQQGLTEPDPRDDLSGKDVMRKLVILAR
                     EAGYNIEPDQVRVESLVPAHCEGGSIDHFFENGDELNEQMVQRLEAAREMGLVLRYVA
                     RFDANGKARVGVEAVREDHPLASLLPCDNVFAIESRWYRDNPLVIRGPGAGRDVTAGA
                     IQSDINRLAQLL"
     gene            complement(7982..9142)
                     /gene="metB"
                     /locus_tag="ACHOSC_RS15220"
                     /old_locus_tag="pgaptmp_003044"
     CDS             complement(7982..9142)
                     /gene="metB"
                     /locus_tag="ACHOSC_RS15220"
                     /old_locus_tag="pgaptmp_003044"
                     /EC_number="2.5.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0019346 - transsulfuration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cystathionine gamma-synthase"
                     /protein_id="HGU4548942.1"
                     /translation="MTRKQATIAVRSGLNDDEQYGCVVPPIHLSSTYNFTGFNEPRAH
                     DYSRRGNPTRDVVQRALAELEGGAGAVLTNTGMSAIHLVMTVFLKPGDLLVAPHDCYG
                     GSYRLFDSLAKRGCYRVLFVDQGDEQALRAALAEKPKLVLVESPSNPLLRVVDIAKIC
                     HLAREVGAVSVVDNTFLSPALQNPLALGADLVLHSCTKYLNGHSDVVAGVVIAKDPDV
                     VTELAWWANNIGVTGGAFDSYLLLRGLRTLVPRMELAQRNAQAIVKYLQTQPLVKKLY
                     HPSLPENQGHEIAARQQKGFGAMLSFELDGDEQTLRRFLGGLSLFTLAESLGGVESLI
                     SHAATMTHAGMAPEARAAAGISETLLRISTGIEDGEDLIADLENGFRAANKG"
     gene            9419..9736
                     /gene="metJ"
                     /locus_tag="ACHOSC_RS15225"
                     /old_locus_tag="pgaptmp_003045"
     CDS             9419..9736
                     /gene="metJ"
                     /locus_tag="ACHOSC_RS15225"
                     /old_locus_tag="pgaptmp_003045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015855437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="met regulon transcriptional regulator MetJ"
                     /protein_id="HGU4548943.1"
                     /translation="MAEWSGEYISPYAEHGKKSEQVKKITVSIPLKVLKILTDERTRR
                     QVNNLRHATNSELLCEAFLHAFTGQPLPDDADLRKERSDEIPEAAKEIMREMGINPET
                     WEY"
     gene            9920..10528
                     /locus_tag="ACHOSC_RS15230"
                     /old_locus_tag="pgaptmp_003046"
     CDS             9920..10528
                     /locus_tag="ACHOSC_RS15230"
                     /old_locus_tag="pgaptmp_003046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YiiX family permuted papain-like enzyme"
                     /protein_id="HGU4548944.1"
                     /translation="MKNRLLILSLLVSVPAFAWQPQTGDIIFQISRSSQSKAIQLATH
                     SDYSHTGMLVMRNKKPYIFEAVGPVKYTPLKQWIAHGEKGKYVVRRVEGGLSVEQQQK
                     LAQTAKRYLGKPYDFSFSWSDDRQYCSEVVWKVYQNALGMRVGEQQKLKEFDLSNPLV
                     QAKLKERYGKNIPLEETVVSPQAVFDAPQLTTVAKEWPLFSW"
     gene            10673..11389
                     /locus_tag="ACHOSC_RS15235"
                     /old_locus_tag="pgaptmp_003047"
                     /pseudo
     CDS             10673..11389
                     /locus_tag="ACHOSC_RS15235"
                     /old_locus_tag="pgaptmp_003047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_086558263.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS1 family transposase"
BASE COUNT         2787 a         3181 c         2876 g         2545 t
ORIGIN      
        1 taaggcgttc acgccgcatc cggcactctg tgccagtaat ctgaaactcc tacgttgttc
       61 aggagttttt tatccgcttc tggacatttt ctctacagaa caatcgttag cccggaaggt
      121 cgaatctgtg actcttattt tcacggacga accagatgaa agcttcactg gcgctgctca
      181 gcctgctaac ggcatttacc agccactcct taaaatctcc cgccgttcca ccaaccgtgg
      241 tacaaatcca ggccaatacc aaccttgcca tcgcagacgg tgccagacag caaattggca
      301 gcacgctatt ttacgatccg gcgtatatgc agcttaccta tcctggcggt gatgtcccgc
      361 aagaacgcgg ggtatgttcc gatgtggtga tccgtgcatt gcgcagccag aaggtcgatt
      421 tgcagaaact ggtgcacgaa gatatggcga agaattttgc cgagtacccg caaaaatggc
      481 agttaaagcg cccggacagc aacatcgatc accgtcgggt gcctaatctg gaaacctggt
      541 ttacccgcca cgataaaaca cgccccatca gcaagaaccc cagtgactat caagcgggcg
      601 atattgtctc ctggcgactg gataacggac tggcacatat tggggtggtg tcagatggct
      661 tcgcccgcga cggtacgccg ctggtgatac ataacattgg cgcaggcgcg caggaagaag
      721 atgtgctgtt tagctggcgg atggtggggc attatcgtta ttttgttaaa taagcgatcg
      781 ccccacatga agaaaaaatt cagatgcttt ttacactgac agcttctttt ttcttacggc
      841 tcaccagcgc cagtgccagc acaatcagca caataccgct accttcaacg gcccccggat
      901 tctcaccgag cagccaccag gaaaagagta cgccgcaaac gggaacggcc agcgtgctta
      961 agctggcaat actggcaggc aagtttttca atacaaacaa ccataagctc cacgccagcg
     1021 ccgtcgccag aatcgcactg taggccagcg cccagaacac ggtgggctgc cagtcaattt
     1081 cacgttgcgg caccagtaaa gcgaccacac tcatcaccag cgccgcatag agcatctgcc
     1141 aggatgttaa cgacaataaa tccacgcgcg gatgacgggc atacagacgt ttagcaacaa
     1201 tcgcgctcgc cccccaactg acgccggaga ggattgccag catggcactt ttcatcgaag
     1261 agaaatcgag ttgccacggc tgcaacacca aaaataagcc gaaagcggca atcagtatcg
     1321 cgaaatattg cccacgtcgc aggcgttcac cgagaaacaa cgcggcgaaa atcaccaccc
     1381 agaacggcat ggtatagctt aggatcgcca ccttccccgc tccgccgctg accagcgccc
     1441 actgcgccag gccaaccatc ccgcaggttt gtaacagggc aatggctaag gtgtatttaa
     1501 acggtgtcgg gcgcattccg cgaccacgta ataaaaggac gatgaataaa acgagagcgc
     1561 cgaaaatgca gcgtaaggcg gtaaagtcga aggcaccgat gtagctggtg acttgcttca
     1621 tgaaaatcca gctataactc cagataagtg tgagcacaac caggccactg attgccagtg
     1681 ggttgctctt tcctgcgaca gacatgagag atccagtgtg tagttttcct gtggacacta
     1741 tacgccggta aagaaacttc agcgactgcc tgaaaggcag tcgctgaaga aggtcagatt
     1801 acagcaggtc gaaacggtcg aggttcatca ctttcaccca tgccgccacg aagtctttaa
     1861 caaacttctc gtgagcatcg ctgctggcgt aaacttcagc caccgcacgc aggacagagt
     1921 tagaaccaaa taccagatcc gcacggctgg cagtgtattt cacttcgccg gtttcgcggt
     1981 cacggccttc aaacagctct ttcgattcgt cggtcgcttt ccactcgtaa cgcatatcca
     2041 gcaagttcac gaagaagtca ttgctcaata cgccaacgcg gtcagtgaag acgccgtttt
     2101 tgctgccatc gaagttggca cccagtacac gcatcccgcc caccagcgcg gtcatttccg
     2161 gcgcggtcag ggtcagttgc tgggctttgt cgatcagcag tgactcggtg gtggaaacat
     2221 ccagacgagc gcgatagtta cggaaaccgt cagcaattgg ctccagcagc tcgaacatct
     2281 caatgtcagt ctgatcctga cgcgcatcaa cgcggcccgg cgcaaacggt acatgaatgc
     2341 tcaaacctgc ggcgcttgcg gctttctcaa caccaaccac accagccagc acgatgatat
     2401 ccgccagcga ggctttacca gactctttct ggattttctc caaaacaggc agagcacgaa
     2461 cggctgcggc gttcacatcc cagtcgcgct gcggcattaa tgccagacgc gcaccgttgg
     2521 caccaccgcg tttgtcgcca ccacggaagg tagaagcaga agcccaggcc accgatacca
     2581 gctcactaac agacagacca gaatccgcaa tcgcgaattt cagatcgata atgtcctgct
     2641 cggtcgggtt gtagatcggc tgcggcagcg gatcttgcca gatcagatct tctttcggca
     2701 cttccggccc gatgtagcga gatttcggcc ccatatccct gtgcgtcagt ttgaaccagg
     2761 cacgggcaaa ggcttcgttg aacgcctgcg gatcgttgag gaaacgacga gagatcttct
     2821 cgaactcagg atcaaaacgc agcgtcaggt cggtcaccag catcgtcggt ttacgtttct
     2881 tcgacggatc aaacggatcc gggataattt ccggtgcgtc taccgcttcg aactggattg
     2941 cgccagccgg gctgcgggtc tgtacccact catacttgaa caggttctcg aagaaatagt
     3001 tgctccactg ggtcggcgtc tgggtccaga ctacttccag accagaggta atggcatctg
     3061 cgccaacgcc gctgccgtaa gtgctcgccc aacctaaacc ttgttcttca atcggtgcag
     3121 cttctggatc aggacctaca tttgatgtcg gaccggcacc gtgggtttta cccagcgtat
     3181 gaccacccgc aataagcgcc acggtttctt cgtcgttcat gcccatgttg ccgaaggtcg
     3241 cacggatagc tgctgccgca gaaagcggtt cgccgctgtg atccgggcct tccgggttaa
     3301 cgtaaatcag gcccatctcg gttgcaccca gcggtgcttt cgccagcgct tccggatgac
     3361 ggtgggtcag ccaggctttt tcatcacccc agttaacatc cagatccggt tcccagacgt
     3421 cttcacgacc ggcaccaaaa ccgaaggtac ggaagccgga gttttccagc gccacgttac
     3481 ccgcgaggat aaacaggtcg gcccaggaga ttttctgacc atatttctgt ttgattggcc
     3541 acaacaggcg acgcgcttta tcgaggctta cgttatccgg ccaggagttc agcggtgcaa
     3601 aacgttgctg accacgaccc gcgccgccgc gtccatcgat tgaacggtaa gtccccgcgc
     3661 cgtgccaggc catacgaata aacagaccgg cgtagctgcc ccagtcggca ggccaccacg
     3721 gctgagattc tgtcagcagg gctttcagat cttttttcag gccgtagtaa tctaatttgc
     3781 tgaattcttt gcggtagtca aagtcctcac ccagcgggtt agaacgatta gaatgttggt
     3841 ttaacaggtc aacacggagt tgatttggcc accagtcgcg agtggttgtg cccgcccccg
     3901 cgctctggtc gtgaccgccc tgatggaacg ggcatttgcc agtggctgtc gtgttatgaa
     3961 tatcgtctga cgtgctcatc aatgtgctcc cctctacagt gttaccgtta cgatacacag
     4021 cgttagagag aagttataat tgaattaatc cacggatgcg atagttgaga tcttacatat
     4081 tgttggttaa agagatgtag atcaaattga tcgtaattaa gcttttttga ttatgtccga
     4141 attcggacaa ctgctttata aaaaagggtt ttagattcct attcttcaag gaaggagggc
     4201 gaaaacaaag ctgacaacct tatttttacg ccctcatttc gaggcagcat tttgtgctct
     4261 gtttaaaatt tgtgatcaca gtgtgatttt cacaaaagcc acactattta taaaccaggt
     4321 cgaacaccca gcgtatggca aatcgcgtaa ctcatttcag cgcggttaag cgtatagaag
     4381 tggaaatctt tcactccttc acggcttaaa atcttcacca tatccatggc aatattcgcg
     4441 cccaccagct tgcgcgtttc agcatcgtca tccagcccgt caaacatttg tgccatccac
     4501 gccggaatac gcacgttggt catatcggca aatttcttcg cctgtttaaa gttagatacc
     4561 ggcaaaatcc ccggaataat ttccacatca atgcccgccg atacgcagcg gtcacgaaaa
     4621 cgcaggtagc tttcgacatc aaagaagaac tgagtaatcg cgcggttggc tccggcatcg
     4681 actttgcgtt tcaggttgag caaatccgcc tgagcgcttt ttgcttccgg gtgaacttcc
     4741 ggatacgccg ccacggagat atcgaaatct gccacttctt ttaacagcgt caccaggtca
     4801 gaagcataca tttccggctt accacttccc ggcggcagat cgccacgcag cgccacgata
     4861 tgacgaatac cgttattcca gtagtcgcgt gcaatggtgc gcagctcgtc gggcgtcgca
     4921 tcaatgcaag taagatgcgg tgccgcttcc agaccagtgc gatctttaat gcctttaata
     4981 atgctgtgcg tacggtcgcg ctcgccggag ttcgcgccat aggtcaccga tacaaacttc
     5041 ggtttcaggc tgctaaggcg atcgatggag ttccacaggg tctgctccat ttcactggta
     5101 cgcggcggga aaaactcgaa cgaaacgtta atctgcccct ggacttctgc caggctctga
     5161 ttcagggcat cccgctggct ggcgtgaaaa aagctcatac cttacctcat caatcgcttg
     5221 tagttatatg ttgtgttgtg cacatctatc catttagacg tccagatgta aagatgaagg
     5281 aaaagccgaa gggcgtcaac cgaaaaatga ccaatgatgg tgaggaaaaa tcagggaaga
     5341 tgaaaaaact tcaggatggt aagaaaaaga aaatccggta tgcaaagcaa accggactgc
     5401 aaatataaac taaccatgta cttattgctt cttgccggat tcgtcgtaaa cgcttcatcc
     5461 ggcctaatcc tatgataatt caatatattt cattagaaac catagacccg aaaacatgag
     5521 taccgggcat tattaaattt ctgaaattac aacaactgtg ccagccggtt gatatccgac
     5581 tgaatcgccc cggcggtgac gtcgcgccca gcgccaggcc cacggatcac cagcggatta
     5641 tcgcggtacc agcggctttc gatggcaaag acgttatcgc atggcagcag tgatgccagc
     5701 ggatggtctt cacgcaccgc ttccacgccc acacgcgctt tgccattggc atcgaaacgc
     5761 gccacgtagc gcagcaccag ccccatttcg cgggccgctt ccagccgttg caccatctgc
     5821 tcgttaagtt catcgccatt ttcaaagaaa tggtcgatgc tgccgccttc gcaatgagca
     5881 ggcaccagcg attccacacg tacctggtcc ggttcaatgt tgtaacccgc ttcacgcgcc
     5941 agaattacca gcttgcgcat tacgtctttg ccggagaggt cgtcacgcgg atcaggctcg
     6001 gttaagccct gctgccacgc ctgatccacc agctcggtaa acggcacgct accgtcgaat
     6061 tgcaggaaca gccaggagag cgtgccggag aagatcccgc tgatcgacaa aatagtatcg
     6121 ccgctgtcga tcagatcgcg cacggtgtgg ttgatcggca agcccgcacc gacggtggca
     6181 ttgtacagcc agtgacgccc ggttttttcg aaggcgtcgt ggatctggcg atatttattg
     6241 ctgtcgctgg ctcccgccag tttgttggcg ctgataacgt ggaaaccgtg gctggcgaaa
     6301 tcaagatact gatcagcaag ctgctggctg gcggtaacgt ccagcaccac taaatcatca
     6361 tacggatggg cgcgcatcca caggaacaac gactcttcat cctgctcaac cgcttcatcg
     6421 ttgaagaagg ctaacgcgcg gctggcgtcc agcccgtcat agctcaacag gctgcggcgg
     6481 ctgtccacca cacctgccag cacaaactca aagccggtac gtgccgaaag cgtgctctgc
     6541 tcacgggcga acagttccag ccaacgggaa ccgatattgc ccttaccgaa caataccagg
     6601 ccgatgcgtt tttctgcgcg gaagacggac tgatgcagcc cctgaatcag gctttcggtc
     6661 gggccggtgc gcagtactgc cactaggctg atgccgtcat cggactgcca ggtaaattcg
     6721 accggctggc ctttcagttg ctgccagaag cggtggcaat gcagcgggtt acgggtgacg
     6781 cctgcaccga ccatcgccac cagcgccagc ccctgacgca ggcgcagttc gccaggtaat
     6841 cccgcttcgt cgaggatttt cagcgcactg tcggccactt ctgaggtgta gcaaaattgc
     6901 agcaactggc gatcgttatg tacgccaacc gccagcgggc gtacctgcgc gcgtttcagg
     6961 atttggtcga tctctttatg cgccagtttg aaatcctgac tgacgggcac ctgaaactca
     7021 atcaaacaga catcatcgtg gctggtgaca atacgcgcac cagtaccgga ggccagcacg
     7081 cgttcaatgc gcgtggaacc ttgatccggc gtgtagctac agcgcagttg caggtcgatt
     7141 tcgctgccag aaaccggctg taaagtacgg gcgtgaagaa cgggagccgc caggcgcgcc
     7201 agttcgctgg cctcatccag acgcagcaac ggcagcaggc aggcatcttt cactttacgc
     7261 gggtcggcac tgtatacccc ggcgacgtcg ctccagatgg ttacgcgaga aacacccgcc
     7321 agcgcaccga tttgtgtcgc ggaatagtcg gaaccgttac gccccagcag caccgtttca
     7381 ccggcgttgt tgcggctgat aaatccggtc accaccagac gtttgcccgg atgttgcacc
     7441 agcagctgtt gcagcaacgg gtaagaaagc ccttcatcaa cctgcggttg tgcggcgcgt
     7501 tcagcgcgta aaaactcgcg ggcatcaagc caggccgctg gcagcccttg ttgattaagt
     7561 accgcagaca tcaggcgtgc cgaccatact tccccgtggc ccaccacttc cgcatacact
     7621 gcgtcgttaa taccgctgtc gagcagcgcc gccaggcgct caaggtcgct gacaaaagcg
     7681 ctaatgaggc tatcggcttc ttcagcgggt agcagaccgc taatcagatc gcactgatag
     7741 cgacgcagcg tttgttgaac ctgatgcgca gagagacgat cggtctggct tagtttcaac
     7801 cagttaatca actggttagt ggtgctaccg gcggcggaaa ccaccatcat atcgtcaggc
     7861 tgagagtact ccgccataat gcccgcgaca cgcaaataac acttcacatc agccagacta
     7921 ctgccaccaa atttatgcag ctgacgacct ttcgcccctg cctgcgcaat cacactcatt
     7981 tttacccctt gtttgcagcc cggaagccat tttccaagtc ggcaattaaa tcttcgccat
     8041 cttcaatacc ggtggagata cgcagcagcg tctcggaaat cccggcggca gcacgcgctt
     8101 ctggtgccat gcctgcatgt gtcatggttg cggcgtgaga gattaaactt tccactcccc
     8161 ctaatgattc cgccagcgta aacaacgaca gcccgcccag gaaacgacgc agcgtctgct
     8221 catcgccatc cagttcaaaa ctcaacattg cgccaaagcc tttttgctga cgcgcggcaa
     8281 tttcatgccc ctgattttcc ggcagcgacg ggtgatacag ttttttcacc aacggctggg
     8341 tttgcagata tttcacaatc gcctgcgcgt tgcgctgcgc cagctccata cgcggcacca
     8401 gcgttcgcaa cccgcgtagc agcagatagc tgtcaaacgc gccgcccgtc acgccaatat
     8461 tgtttgccca ccaggcaagt tcagtgacaa cgtccgggtc tttagcaatc accacgccag
     8521 ccaccacgtc agagtgaccg ttcagatatt tcgtacatga atgcaacacc agatcggcac
     8581 ccaatgccag cggattttgt aatgccgggc ttaagaaggt gttatccacc acgctcaccg
     8641 ccccgacttc ccttgccaga tggcagattt tcgcaatatc cacgacgcgt aacaatggat
     8701 tacttgggct ttctaccagt accagtttgg gtttttctgc cagtgctgcc cgtaatgcct
     8761 gttcatcgcc ttgatcaaca aacaacacgc gatagcaacc gcgtttcgcc aggctgtcga
     8821 acaggcgata gctaccgccg tagcagtcgt gcggcgcaac cagcagatcg ccaggtttca
     8881 aaaagacggt cattaccagg tgaatcgcgg acatgccggt attagtaagt actgcaccag
     8941 caccgccttc cagttctgcc agcgcacgct gaaccacatc gcgcgttggg ttgccgcgac
     9001 gcgagtaatc atgcgcgcgc ggttcattaa atccggtaaa gttatatgtg ctggaaagat
     9061 ggatcggtgg gacaacgcaa ccatactgtt cgtcgtcatt taacccgcta cgcactgcga
     9121 tggtggcctg tttacgcgtc atgtgatgaa gttccctggg ctttgtcggt gaaatgtcag
     9181 gcaccagagt aaacattgtg ttaatggacg tcaatacatc tggacatcta aacttctttg
     9241 cgtatagatt gagcaaatcc caaatagccg ttaaaattat atgcattatc acgccgacag
     9301 gtgcattaca cgatgtcacg gtaacgcctg tacggtaaac tatgcgggtt tacggtcagt
     9361 acccacataa attgtgtggt ctggtctcaa tttattgacg aagaggatta agtatctcat
     9421 ggctgaatgg agcggcgaat atatcagccc atacgctgag cacggcaaga agagtgaaca
     9481 agtcaaaaag attacggttt ccattcctct taaggtgtta aaaatcctca ccgatgaacg
     9541 cacgcgtcgt caggtgaaca acctgcgtca cgctaccaac agcgagctgc tgtgcgaagc
     9601 gtttctgcat gcctttaccg ggcaaccttt gccggatgat gccgatctgc gtaaagagcg
     9661 cagcgacgaa atcccggaag cggcaaaaga gatcatgcgt gagatgggga ttaacccgga
     9721 gacgtgggaa tactaaagcg gaaatgagcg gcgcggagtg gaatcgcctg atgcgctacg
     9781 cttatcaggc ctacgtcata ttgcaattta ttgaatttgc acgaacttgt aggccggata
     9841 aggcgttcac gccgcatccg gcataaacaa cgagcacgtt gtctgcgacc caccgctttt
     9901 tatacatgga cgtttaacta tgaaaaacag gctgctgatc ctcagcctgc tggtttctgt
     9961 acctgccttt gcctggcagc cgcaaactgg cgacatcatc tttcaaatct ctcgctcatc
    10021 gcaaagtaaa gcgatccaac tggcaaccca ttccgattac agccacaccg gtatgctggt
    10081 gatgcgtaac aaaaagccct acatttttga agcggtcggc ccggtgaaat acaccccgct
    10141 caagcagtgg attgcccatg gtgaaaaggg caaatacgtc gttcgccgcg ttgaaggcgg
    10201 actgagtgtt gaacaacagc aaaaactggc gcaaacggca aaacgttatc tcggtaaacc
    10261 gtatgatttc agcttctcat ggagcgacga tcgccagtac tgttcggaag tggtgtggaa
    10321 ggtttaccag aacgcgctgg gaatgcgcgt gggcgaacag cagaagttga aagagtttga
    10381 tctcagcaac ccgctggtcc aggcgaagct caaagaacgc tacggtaaaa atattccgct
    10441 tgaggaaacg gtagtttcac cacaagccgt tttcgacgcg ccacaactca ccacggttgc
    10501 caaagagtgg ccgctgtttt cgtggtaatg gatgatgaaa aaaataattc ccgccgtagt
    10561 ctgtttgttg ctttgtagtt gcacgacctt tccgcaagct gtcgctccgg taaacggtga
    10621 tgcctccaat tagttgaatt aaggatataa tactcgcttt taaggttgtt tcatggtcac
    10681 agttaacctt cattgccccc gttgccagtc cgttcaggtt taccgtcacg ggcagaaccc
    10741 aaaaagacat gatcgattcc gcggccgtga ctgtcaccgg gtatttcagc tcagttactg
    10801 ttacgagccc cgtaaaccgg gagttaaaga ccaaatcgct gaaatgactt tcaacggtgc
    10861 cggggttcgc gatactgccc gtacgctgaa aatcggcatt aatacagtca tccgaacttt
    10921 aaaaaactct cgccacgaag aataacgtca tccccggtcg ctcatgctga tgtggcactc
    10981 atctgcaaac ttagtcagca atggagcttt gtcggcagta aagcgcgaca gcactggctc
    11041 tggtacgtgt acaacacgaa aactggtggt gttctggcct acacttttgg cccccggact
    11101 gacgaaacct gtcgtgaatt gcgggcgctg cttacccttt taccatcggc atgttgacca
    11161 ctgacgaatg gggtagttac gctagagagt taccgaagga gaagcatctg actggtaaga
    11221 tttttactca gcacattgag cgtaacaatc tgaccctgag aacccgaatc aaacgtctgg
    11281 cccgcagaac catctgtttc tcgcgttctg tagaacttca tgaaaaagtc atcggtgctt
    11341 ttatcgaaaa tatatgttct actaattgga gtcatcaccc ttctgataa
//
LOCUS       DAZKHF020000141        11383 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2201S31241
            SAMN43267800-rid25106413.denovo.142, whole genome shotgun sequence.
ACCESSION   DAZKHF020000141 DAZKHF020000000
VERSION     DAZKHF020000141.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267800
            Sequence Read Archive: SRR30307263
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 11383)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11383)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 11383)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350581.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350581.2
            Genome Coverage       :: 151x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:21:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,652
            CDSs (total)                      :: 5,557
            Genes (coding)                    :: 5,320
            CDSs (with protein)               :: 5,320
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 237
            CDSs (without protein)            :: 237
            Pseudo Genes (ambiguous residues) :: 0 of 237
            Pseudo Genes (frameshifted)       :: 102 of 237
            Pseudo Genes (incomplete)         :: 130 of 237
            Pseudo Genes (internal stop)      :: 52 of 237
            Pseudo Genes (multiple problems)  :: 41 of 237
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11383
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267800-rid25106413.denovo.142"
                     /serotype="O 26:H11"
                     /isolate="2201S31241"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-01"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            317..1888
                     /gene="garD"
                     /locus_tag="ACHOSC_RS15240"
                     /old_locus_tag="pgaptmp_003048"
     CDS             317..1888
                     /gene="garD"
                     /locus_tag="ACHOSC_RS15240"
                     /old_locus_tag="pgaptmp_003048"
                     /EC_number="4.2.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008867 - galactarate dehydratase activity
                     [Evidence IEA];
                     GO_process: GO:0046392 - galactarate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="galactarate dehydratase"
                     /protein_id="HGU4548945.1"
                     /translation="MANIEIRQETPTAFYIKVHDTDNVAIIVNDNGLKAGTRFPDGLE
                     LIEHIPQGHKVALLDIPANGEIIRYGEVIGYAVRAIPRGSWLDESMVVLPEAPPLHTL
                     PLATKVPAPLPPLEGYTFEGYRNADGSVGTKNLLGITTSVHCVAGVVDYVVKIIERDL
                     LPKYPNVDGVVGLNHLYGCGVAINAPAAVVPIRTIHNISLNPNFGGEVMVIGLGCEKL
                     QPERLLVGTDDVQAIPVESASIVSLQDEKHVGFQSMVEDILQVAERHLQKLNQRQRET
                     CPASELVVGMQCGGSDAFSGVTANPAVGYASDLLVRCGATVMFSEVTEVRDAIHLLTP
                     RAVNEEVGKRLLEEMEWYDNYLNMGKTDRSANPSPGNKKGGLANVVEKALGSIAKSGK
                     SAIVEVLSPGQRPTKRGLIYAATPASDFVCGTQQVASGITVQVFTTGRGTPYGLMAVP
                     VIKMATRTELANRWFDLMDINAGTIATGEETIEEVGWKLFHFILDIASGKKKTFSDQW
                     GLHNQLAVFNPAPVT"
     gene            2037..2372
                     /gene="prlF"
                     /locus_tag="ACHOSC_RS15245"
                     /old_locus_tag="pgaptmp_003049"
     CDS             2037..2372
                     /gene="prlF"
                     /locus_tag="ACHOSC_RS15245"
                     /old_locus_tag="pgaptmp_003049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system antitoxin PrlF"
                     /protein_id="HGU4548946.1"
                     /translation="MPANARSHAVLTTESKVTIRGQTTIPAPVREALKLKPGQDSIHY
                     EILPGGQVFMCRLGDEQEDHTMNAFLRFLDADIQNNPQKTRPFNIQQGKKLVAGMDVN
                     IDDEIGDDE"
     gene            2372..2836
                     /gene="yhaV"
                     /locus_tag="ACHOSC_RS15250"
                     /old_locus_tag="pgaptmp_003050"
     CDS             2372..2836
                     /gene="yhaV"
                     /locus_tag="ACHOSC_RS15250"
                     /old_locus_tag="pgaptmp_003050"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system ribonuclease
                     toxin YhaV"
                     /protein_id="HGU4548947.1"
                     /translation="MDFPQRVNGWALYAHPCFQETYDALVAEVETLKGKDPENYQRKA
                     ATKLLAVVHKVIEEHITVNPSSPAFRHGKSLGSGKNKDWSRVKFGAGRYRLFFRYSEK
                     EKVIILGWMNDENTLRTYGKKTDAYTVFSKMLKRGHPPADWETLTRETEETH"
     gene            complement(2891..3700)
                     /gene="agaR"
                     /locus_tag="ACHOSC_RS15255"
                     /old_locus_tag="pgaptmp_003051"
     CDS             complement(2891..3700)
                     /gene="agaR"
                     /locus_tag="ACHOSC_RS15255"
                     /old_locus_tag="pgaptmp_003051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aga operon transcriptional regulator AgaR"
                     /protein_id="HGU4548948.1"
                     /translation="MSNTDASGEKRVTGTSERREQIIQRLRQQGSVQVNDLSALYGVS
                     TVTIRNDLAFLEKQGIAVRAYGGALICDSTTPSVEPSVEDKSALNTAMKRSVAKAAVE
                     LIQPGHRVILDSGTTTFEIARLMRKHTDVIAMTNGMNVANALLEAEGVELLMTGGHLR
                     RQSQSFYGDQAEQSLQNYHFDMLFLGVDAIDLERGVSTHNEDEARLNRRMCEVVERII
                     VVTDSSKFNRSSLHKIIDTQRIDMIIVDEGIPADSLEGLRKAGVEVILVGE"
     gene            3949..5229
                     /gene="kbaZ"
                     /locus_tag="ACHOSC_RS15260"
                     /old_locus_tag="pgaptmp_003052"
     CDS             3949..5229
                     /gene="kbaZ"
                     /locus_tag="ACHOSC_RS15260"
                     /old_locus_tag="pgaptmp_003052"
                     /EC_number="4.1.2.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagatose-bisphosphate aldolase subunit KbaZ"
                     /protein_id="HGU4548949.1"
                     /translation="MKHLTEMVRQHKAGKTNGIYAVCSAHPLVLEAAIRYASANQTPL
                     LIEATSNQVDQFGGYTGMTPADFRGFVCQLADSLNFPQDALILGGDHLGPNRWQNLPA
                     AQAMANADDLIKSYVAAGFKKIHLDCSMSCHDDPIPLTDDIVAERAARLAKVAEETCL
                     EHFGEADLEYVIGTEVPVPGGAHETLSELAVTTPDAARATLEAHRHAFEKQGLNAIWP
                     RIIALVVQPGVEFDHTNVIDYQPAKASALSQMVENYETLIFEAHSTDYQTPQSLRQLV
                     IDHFAILKVGPALTFALREALFSLAAIEEELVPAKACSGLRQVLEDVMLDRPEYWQSH
                     YHGDGNARRLARGYSYSDRVRYYWPDSQIDDAFAHLVRNLADSPIPLPLISQYLPLQY
                     VKVRSGELQPTPRELIINHIQDILAQYHTACEGQ"
     gene            5252..5725
                     /gene="agaV"
                     /locus_tag="ACHOSC_RS15265"
                     /old_locus_tag="pgaptmp_003053"
     CDS             5252..5725
                     /gene="agaV"
                     /locus_tag="ACHOSC_RS15265"
                     /old_locus_tag="pgaptmp_003053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312038.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS N-acetylgalactosamine transporter subunit
                     IIB"
                     /protein_id="HGU4548950.1"
                     /translation="MPNIVLSRIDERLIHGQVGVQWVGFAGANLVLVANDEVAEDPVQ
                     QNLMEMVLAEGIAVRFWTLQKVIDNIHRAADRQKILLVCKTPADFLTLVKGGVPVNRI
                     NVGNMHYANGKQQIAKTVSVDAGDIAAFNDLKAAGVECFVQGVPTEPAVDLFKLL"
     gene            5736..6515
                     /gene="agaW"
                     /locus_tag="ACHOSC_RS15270"
                     /old_locus_tag="pgaptmp_003054"
     CDS             5736..6515
                     /gene="agaW"
                     /locus_tag="ACHOSC_RS15270"
                     /old_locus_tag="pgaptmp_003054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS N-acetylgalactosamine transporter subunit
                     IIC"
                     /protein_id="HGU4548951.1"
                     /translation="MEISLLQAFALGIIAFIAGLDMFNGLTHMHRPVVLGPLVGLVLG
                     DLHTGILTGGTLELVWMGLAPLAGAQPPNVIIGTIVGTAFAITTGVKPDVAVGVAVPF
                     AVAVQMGITFLFSVMSGVMSRCDRMAENADTRGIERVNYLALLALGTFYFLCAFLPIY
                     FGAEHAKTIIDVLPQRLIDGLGVAGGIMPAIGFAVLLKIMMKNVYIPYFILGFVAAAW
                     LKLPVLAIAAAALAMALIDLLRKSPEPTQPAAQKEEFEDGI"
     gene            6505..7383
                     /gene="agaE"
                     /locus_tag="ACHOSC_RS15275"
                     /old_locus_tag="pgaptmp_003055"
     CDS             6505..7383
                     /gene="agaE"
                     /locus_tag="ACHOSC_RS15275"
                     /old_locus_tag="pgaptmp_003055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019079236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS N-acetylgalactosamine transporter subunit
                     IID"
                     /protein_id="HGU4548952.1"
                     /translation="MASNQTTLPNVSENEETLLTGVNENVYEDQSIGAELTKKDINRV
                     AWRSMLLQASFNYERMQASGWLYGLLPALKKIHTNKRDLARAMKGHMGFFNTHPFLVT
                     FVIGIILAMERSKQDVNSIQSTKIAVGAPLGGIGDAMFWLTLLPICGGIGASLALQGS
                     ILGAVVFILLFNVVHLGLRFGLAHYAYRMGVAAIPLIKANTKKVGHAASIVGMTVIGA
                     LVATYVRLSTTLEITAGDAVVKLQADVIDKLMPAFLPLVYTLTMFWLVRRGWSPLRLI
                     AVTVVLGIVGKFCHFL"
     gene            7401..7835
                     /gene="agaF"
                     /locus_tag="ACHOSC_RS15280"
                     /old_locus_tag="pgaptmp_003056"
     CDS             7401..7835
                     /gene="agaF"
                     /locus_tag="ACHOSC_RS15280"
                     /old_locus_tag="pgaptmp_003056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS galactosamine/N-acetylgalactosamine
                     transporter subunit IIA"
                     /protein_id="HGU4548953.1"
                     /translation="MLSIILTGHGGFASGMEKAMKQILGEQSQFIAIDFPETSSTALL
                     TSQLEEAIAQLDCEDGIVFLTDLLGGTPFRVASTLALQKPGCEVITGTNLQLLLEMVL
                     EREGLSGEEFRVQALECGHRGLTSLVDELGRCHEECPVEEGI"
     gene            7832..8965
                     /gene="nagA"
                     /locus_tag="ACHOSC_RS15285"
                     /old_locus_tag="pgaptmp_003057"
     CDS             7832..8965
                     /gene="nagA"
                     /locus_tag="ACHOSC_RS15285"
                     /old_locus_tag="pgaptmp_003057"
                     /EC_number="3.5.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312042.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008448 - N-acetylglucosamine-6-phosphate
                     deacetylase activity [Evidence IEA];
                     GO_process: GO:0006044 - N-acetylglucosamine metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylglucosamine-6-phosphate deacetylase"
                     /protein_id="HGU4548954.1"
                     /translation="MTHVLRARRLLTEEGWLDDHQLRIADGVIAAIEPIPVGVTERDA
                     ELLCPAYIDTHVHGGAGVDVMDDAPDVLDKLAMHKAREGVGSWLPTTVTAPLNTIHAA
                     LKRIAQRCQRGGPGAQVLGSYLEGPYFTPQNKGAHPPELFRELEIAELDQLIAVSQHT
                     LRVVALAPEKEGALQAIRHLKQQNVRVMLGHSAATWQQTRAAFDAGADGLVHCYNGMT
                     GLHHREPGMVGAGLTDKRAWLELIADGHHVHPAAMSLCCCCAKERIVLITDAMQAAGM
                     PDGRYTLCGEEVQMHGGVVRTASGGLAGSTLSVDAAVRNMVELTGVTPAEAIHMASLH
                     PARMLGVDGVLGSLKPGKRASIVALDSGLHVQQIWIQSQLASF"
     gene            9316..10470
                     /locus_tag="ACHOSC_RS15290"
                     /old_locus_tag="pgaptmp_003058"
     CDS             9316..10470
                     /locus_tag="ACHOSC_RS15290"
                     /old_locus_tag="pgaptmp_003058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AgaS family sugar isomerase"
                     /protein_id="HGU4548955.1"
                     /translation="MPENYTPAAAATGTWTEEEIRHQPRAWIRSLTNIDALRSALNNF
                     LEPLLRKENLRIILTGAGTSAFIGDIIAPWLASHTGKNFSAVPTTDLVTNPMDYLNPA
                     HPLLLISFGRSGNSPESVAAVELANQFVPECYHLPITCNEAGALYQNAINSDNAFALL
                     MPAETHDRGFAMTSSITTMMASCLAVFAPETINSQTFRDVADRCQAILTSLGDFSEGV
                     FGYAPWKRIVYLGSGGLQGAARESALKVLELTAGKLAAFYDSPTGFRHGPKSLVDNET
                     LVVVFVSSHPYTRQYDLDLLAELRRDNQAMRVIAIAAESTDIVAAGPHIILPPSRHFI
                     DVEQAFCFLMYAQTFALMQSLHMGNTPDTPSASGTVNRVVQGVIIHPWQA"
     gene            10483..11343
                     /gene="kbaY"
                     /locus_tag="ACHOSC_RS15295"
                     /old_locus_tag="pgaptmp_003059"
     CDS             10483..11343
                     /gene="kbaY"
                     /locus_tag="ACHOSC_RS15295"
                     /old_locus_tag="pgaptmp_003059"
                     /EC_number="4.1.2.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagatose-bisphosphate aldolase subunit KbaY"
                     /protein_id="HGU4548956.1"
                     /translation="MSIISTKYLLQDAQANGYAVPAFNIHNAETIQAILEVCSEMRSP
                     VILAGTPGTFKHIALEEIYALCSAYSTTYNMPLALHLDHHESLDDIRRKVHAGVRSAM
                     IDGSHFPFAENVKLVKSVVDFCHSQDCSVEAELGRLGGVEDDMSVDAESAFLTDPQEA
                     KRFVELTGVDSLAVAIGTAHGLYSKTPKIDFQRLAEIREVVDVPLVLHGASDVPDEFV
                     RRTIELGVTKVNVATELKIAFAGAVKAWFAENPQGNDPRYYMRVGMDAMKEVVRNKIN
                     VCGSANRISA"
BASE COUNT         2732 a         2942 c         2997 g         2712 t
ORIGIN      
        1 gagtttcatt tatgccgtaa cggcaatgaa ctcgggaatt agtataagca gcgcgagaat
       61 aataatcatt gtgcaaatgc taatttaatt aatgctattt aaatattatt ttgagcatat
      121 gcacataagg ttgcgcgcta aagcacagat ttgcgcttta ccttgccggg cggcactgca
      181 atccctgaaa tgattgacat tgatcacatt tctgcgttta aactcctgac attcttattt
      241 cacccaatga agtcatttat ttttaaatga gaccaggtcc tcattttaat aacccctggc
      301 tggagaatat agtacaatgg ccaacatcga aatcagacaa gaaacgccaa ctgcgtttta
      361 tataaaagtt cacgacacag ataatgtggc aattattgtt aatgataatg gcctgaaagc
      421 aggaacgcgt tttccggatg gactggaatt aattgaacat attccccagg ggcataaagt
      481 cgcattgctg gacattccgg ctaatggtga aattattcgt tatggtgaag tgattggtta
      541 cgccgtgcgt gcaatcccac gcggaagctg gctcgacgaa tcaatggttg tactaccgga
      601 agcgccgccg ttacacacgc tgccactggc aaccaaagtc ccggccccct taccgccgct
      661 ggaaggatac acctttgagg gctatcgcaa tgccgatggc agcgtgggca ccaaaaacct
      721 gctcggcatc accaccagcg tccactgtgt ggcaggcgtg gtggactatg tagtaaaaat
      781 cattgaacgc gatctgctac cgaaataccc gaacgtcgat ggcgtggtgg ggctgaatca
      841 tttgtacggc tgtggcgtgg cgattaacgc accggcggca gttgtgccta ttcgtaccat
      901 ccacaatatt tcgctgaacc ctaactttgg cggcgaagta atggtgattg gtctgggttg
      961 tgaaaagttg cagcctgagc gcctgctggt cggcacggat gatgtgcaag ctattccagt
     1021 agaaagcgcc agcattgtca gtttgcagga tgaaaagcat gtcggttttc agtccatggt
     1081 cgaggatatt ttgcaggtcg ccgaacgcca tctacaaaaa ctgaatcaac gtcagcgaga
     1141 aacctgtccg gcttcagaac tggttgtcgg catgcagtgc ggtggcagcg atgccttttc
     1201 tggcgtaacg gcaaacccgg cggttggcta tgcgtctgat ctactggtgc gctgcggcgc
     1261 aacggtgatg ttctcagaag tcacggaagt gcgtgacgcg atccatctgc tgacaccacg
     1321 cgcagtgaac gaagaggtcg gcaaacggct gctggaggag atggagtggt acgataacta
     1381 tctcaatatg ggaaaaaccg accgcagcgc caacccttcg ccgggcaaca agaaaggcgg
     1441 tctggcaaac gtggtagaga aggcactcgg ctccattgct aaatcgggta aaagcgcaat
     1501 tgttgaagtg ctgtcgcccg gtcaacgccc gactaaacgc ggattaattt acgccgcgac
     1561 gccagccagc gattttgtct gtggcacgca acaggtggct tcgggtatca ccgtgcaagt
     1621 gtttacgacc ggtcgtggta cgccgtacgg cctgatggcg gtacccgtca ttaaaatggc
     1681 gacccgcacc gagctggcga accgctggtt tgatttaatg gatattaacg cgggcactat
     1741 cgccaccggc gaagaaacca ttgaagaggt gggctggaag ttgttccact ttattctcga
     1801 catcgccagc gggaagaaga aaaccttctc ggatcaatgg gggctgcata accagctggc
     1861 agtgtttaac ccggcaccgg tgacctgatt tcttttggaa cgtccctcgc aaaacatggc
     1921 cttagtgcca tgtttttatt gtttaaagcc cccacgtcca ttaataatgc atttgcatta
     1981 ccttagttca agcttataat ttgagcagaa aacaggctgt aaaaggacag tgaatcatgc
     2041 ccgctaatgc tcgctctcac gctgtactga ccactgaatc aaaggtcacg atacgcggac
     2101 aaacaactat ccccgcacca gtgcgtgagg ccttaaaact gaagccaggc caggacagca
     2161 ttcattacga aattctgcct ggtgggcaag tctttatgtg ccgactggga gatgaacagg
     2221 aggatcatac tatgaatgca tttttgcgtt ttctggatgc agatatccag aacaacccgc
     2281 aaaaaactcg tccattcaac attcaacaag gaaagaaact tgtcgctggc atggacgtca
     2341 acattgatga tgagattggc gacgacgaat aatggatttt ccacaaaggg ttaatggttg
     2401 ggcgctatat gctcatccct gttttcagga aacctacgac gctttagttg ccgaagtcga
     2461 gacattaaag ggaaaagatc ctgaaaatta tcagagaaaa gccgccacaa agttattggc
     2521 ggtagtccat aaagtgattg aggagcatat cacggtcaat ccatcatcac cggcattccg
     2581 tcatggcaag tcgttaggct ctgggaaaaa taaagactgg tcacgggtaa aatttggtgc
     2641 tggtcgttat cgtctcttct ttcgttatag tgaaaaagag aaagtcatca ttctgggatg
     2701 gatgaacgat gaaaacactc tgcgcaccta cggtaaaaaa acagatgcct ataccgtatt
     2761 cagcaaaatg ttaaaaagag gacatcctcc tgccgactgg gaaaccctca cccgagaaac
     2821 agaagaaacc cattgatggt gttcacaatg cccgccgaac gtattcgtgt tgaccagcgg
     2881 gcaccctttc tcactccccg accagaatca cttcaacccc agcctttcgc agtccttcca
     2941 gactatccgc aggaatgcct tcatcaacaa tgatcatgtc gatacgttga gtatcaatga
     3001 tcttatgtaa actggaacga ttgaatttac tggaatcggt gaccacgatg atccgttcca
     3061 caacttcgca catccgacgg tttaaacggg cttcatcttc attatgtgtg ctgacgccgc
     3121 gctccagatc gatcgcatct acaccaagaa acagcatatc gaagtggtaa ttttgcagcg
     3181 attgctcagc ctgatcgccg taaaaagatt gcgactgacg gcgcaaatgc ccgccggtca
     3241 tcagcagctc aacgccttcc gcttccagca acgcattagc cacgttcata ccgttggtca
     3301 tcgcaattac gtcagtgtgc ttacgcatca gacgagcaat ctcaaaagtg gtagtcccgg
     3361 aatcgaggat cacccgatga ccaggctgaa tcaactcaac ggcagctttc gcaacgctgc
     3421 gtttcatcgc ggtgttcagc gcgcttttat cttccactga tggctcgact gacggcgtcg
     3481 tgctatcgca gatcaacgcg ccaccatagg cacgcacagc gatcccctgc ttttccagaa
     3541 acgccagatc attgcggatc gtcacagtag atacgccata caatgccgac agatcgttaa
     3601 cctgcacact cccttgctgt cgcagacgct gaatgatctg ttctcgtcgc tcgctggtgc
     3661 cagtcactcg cttctcacct gaagcgtcgg tattactcat agtaagtcct ttcgtaaaac
     3721 tttcgtttca tttcgttttg cctattaacg cctttctatt aagcaaatgc aagcccacct
     3781 tgcccattga cgcaagctac tctcgtttca gtgactttca ttatgtttct tttgtgaatc
     3841 agatcagaaa actattatct ttcgttttat ttttatctca ccatgacgca gtatcaactg
     3901 aaacaaaacg aaagattaat atcgcagtaa tctgaactgg agaggaaagt gaaacatctg
     3961 acagaaatgg tgagacagca caaagcgggc aaaacaaatg gaatttatgc cgtttgttcc
     4021 gcacatccgc tggtgctgga ggctgcaatc cgctacgcca gtgcaaacca aacgccgtta
     4081 ctgattgaag caacctccaa tcaggtagac cagttcggcg gttataccgg aatgacgccc
     4141 gccgattttc gcggctttgt ttgtcagctc gccgactcgt tgaatttccc gcaggatgcg
     4201 ttgattctgg gtggtgacca tctggggcca aaccgctggc aaaacctgcc ggccgctcag
     4261 gcaatggcca atgccgatga tttgattaaa agctacgttg cggcaggatt caaaaaaatc
     4321 caccttgatt gcagcatgtc ctgtcatgac gatccgattc ccttaactga tgacatcgtg
     4381 gctgaacgcg ccgcccgtct ggcgaaagtg gcggaagaaa cctgtcttga acactttggc
     4441 gaagccgatc tggagtatgt cattggtacc gaagtgccgg tacctggcgg cgcgcatgaa
     4501 accttaagcg agctggcggt caccacgccg gatgccgccc gcgccacgct ggaagcccat
     4561 cgtcacgcct ttgaaaagca aggtttgaat gccatctggc cacgcatcat tgccctggtg
     4621 gttcaacccg gcgtcgaatt cgatcacacc aacgttattg attatcagcc cgccaaagcg
     4681 agcgccttaa gccagatggt cgaaaactac gaaacgctga ttttcgaagc gcactctacc
     4741 gattatcaaa cgccgcaatc gctgcgccag ctggtgattg accactttgc cattctgaaa
     4801 gttggcccag cgctgacctt cgccctgcgt gaagctctgt tctctctggc ggcgattgaa
     4861 gaagaactgg tgccagcgaa agcctgttct ggtctgcgtc aggtgctgga agacgtgatg
     4921 ctcgaccgcc cggaatactg gcaaagccac taccacggtg acggcaacgc gcgtcgtctg
     4981 gcgcgtggtt atagctactc ggatcgcgtg cgctattact ggccggacag ccagattgat
     5041 gacgctttcg ctcatctggt tcgtaatctg gcggattcac caattccgct accgctcatc
     5101 agccagtatc tgccgctgca atacgtgaaa gttcgctccg gcgagctgca accgacgcca
     5161 cgggaactca ttatcaacca tattcaggac atcctggcgc agtaccacac agcctgtgaa
     5221 ggccaataag caaaacaaag aggaacacgc tatgccaaat attgttttaa gccggattga
     5281 tgaacgcttg attcacggtc aggtcggcgt tcagtgggtc ggatttgcgg gggcaaatct
     5341 ggtgctggta gccaacgatg aggttgccga agatccggta caacaaaacc tgatggaaat
     5401 ggtactggca gaagggatcg ccgtacgttt ctggacgctg caaaaagtta tcgacaacat
     5461 tcatcgcgcc gccgatcgac agaaaatcct gctggtttgt aaaacacccg ccgatttcct
     5521 gacgctggtg aaaggtggcg ttccggtgaa tcgcattaac gttggcaata tgcactacgc
     5581 caatggcaaa caacaaatcg ccaaaacggt ttctgtggat gcgggcgata tcgcagcatt
     5641 taacgacctg aaagccgctg gggtggaatg cttcgttcag ggcgtcccga cagagcctgc
     5701 tgtagacctc tttaaattac tttgagggat tcatcatgga aatcagcctg ttgcaggcat
     5761 ttgcgttggg cattatcgcc tttatcgctg gcctggatat gtttaacggc ctaacacata
     5821 tgcaccgccc ggtggtcctc ggcccgttgg tcgggctggt acttggcgat ctgcataccg
     5881 gaattttaac cggcggtacg ctggaactgg tgtggatggg gctggccccg ctggcgggcg
     5941 cacagccgcc taacgtgatt atcggtacta tcgtcggcac ggcgtttgcc attactactg
     6001 gcgtgaaacc cgatgtcgcg gtcggtgtcg ccgtaccttt cgctgtcgca gtacagatgg
     6061 ggattacctt cctgttctcg gtgatgtctg gcgtgatgtc tcgctgcgac cggatggcgg
     6121 agaacgccga tactcgcggc attgaacgcg tgaactatct ggcactgctg gcactcggca
     6181 ctttctattt tctctgcgct ttcctgccta tctacttcgg cgcggaacat gcaaaaacca
     6241 tcattgatgt cctgccgcaa cgattaatcg acggcctcgg cgtcgcgggc ggcatcatgc
     6301 cagcaatcgg ttttgccgtg ctgctgaaaa tcatgatgaa aaacgtctac atcccctact
     6361 tcatcctggg ctttgttgcc gcagcctggc tcaagttacc ggtgctggct attgctgccg
     6421 ctgcgctggc gatggcgctg atcgacctgc tgcgtaaatc tcctgaaccg actcaacctg
     6481 cggcacagaa agaggaattt gaagatggca tctaatcaaa ccaccctgcc gaacgtcagt
     6541 gaaaacgaag aaacattgct gactggcgtc aatgaaaacg tgtatgaaga tcagagcatt
     6601 ggcgcggagc tgacgaaaaa agatatcaac cgcgtcgcct ggcgttccat gctgttacaa
     6661 gcgtctttta actacgaacg tatgcaggct tctggctggt tgtacggtct gctgcctgct
     6721 ctgaaaaaga tccacactaa taaacgcgac ctggcgcgcg ccatgaaggg gcatatgggt
     6781 ttcttcaata cccatccgtt tctggtgaca tttgttatcg gcattatcct tgcgatggag
     6841 cgttctaagc aggacgttaa cagtattcag agcaccaaaa ttgccgtcgg tgcgccgctc
     6901 ggcgggattg gcgatgcaat gttctggcta acgctactgc cgatttgtgg cgggattgga
     6961 gccagtctgg cattgcaagg ctccattctt ggcgcagtcg tctttattct gctgttcaac
     7021 gtggtacacc tggggctgcg ttttggtctg gcgcattatg cttaccgcat gggcgtggcg
     7081 gcgattccac tcattaaagc gaataccaaa aaagtcggcc atgctgcgtc tatcgttggg
     7141 atgacggtaa tcggtgcgct ggtggcaacc tatgttcgtt taagcaccac gctggaaatc
     7201 acagcgggcg acgcagtggt taagttacag gctgatgtta tcgacaaact gatgcctgcc
     7261 ttcttaccgt tggtctacac cctgaccatg ttttggttgg tacgccgcgg ctggagtccg
     7321 ctgcgtctga ttgcagttac cgtggttctc ggcatcgtcg gtaaattctg ccatttcctt
     7381 taaatacaat gaggtattag atgttaagta ttattttgac agggcatggc gggtttgcca
     7441 gcggcatgga aaaagcaatg aagcaaattc tcggtgagca atcgcagttt atcgccatcg
     7501 attttccgga aacctcaagc accgcgctgc ttacatcgca gcttgaagag gccatcgccc
     7561 aactggattg tgaagacggc attgtttttc tgacggattt gctgggcggc acgccgtttc
     7621 gcgtagcttc aacgctggcg ctgcaaaaac cgggctgtga agtgatcacc ggcaccaatt
     7681 tgcaactgct actggagatg gtgctggagc gcgaagggtt aagcggtgaa gaattccgtg
     7741 tacaggcgct ggagtgtgga catcgtgggc tgaccagtct ggtggacgag ttaggccgct
     7801 gccatgaaga atgtccggtc gaggaaggga tatgacacac gttctgcgcg ccagaaggct
     7861 gctgactgaa gagggatggc tcgatgacca tcagttgcgt attgctgacg gtgtcatcgc
     7921 agcaatcgaa ccgattccag tgggcgtgac tgaacgcgat gcggaactgc tctgccccgc
     7981 ttacatcgac acccatgtac acggtggtgc gggcgttgat gtaatggatg acgcgccgga
     8041 tgtactcgac aagctggcaa tgcacaaggc acgcgaaggt gtcggcagtt ggttgccgac
     8101 taccgtaacc gcgccgctta acaccattca tgcggcgctg aaacgtattg ctcaacgttg
     8161 ccaacgcggc ggacctggtg cgcaagtgct ggggagttat ctcgaaggac cgtacttcac
     8221 gccgcagaat aaaggcgcgc atccgccgga attgtttcgc gagcttgaaa ttgccgagct
     8281 ggatcaattg attgccgttt ctcagcacac cttacgcgtg gtagcgctgg caccggaaaa
     8341 agagggggca ttgcaggcca tccgccatct taaacagcaa aatgtacgag tgatgctggg
     8401 gcatagcgcg gcgacctggc aacaaactcg cgccgcgttt gatgctggtg ccgacggcct
     8461 ggtgcattgc tataacggga tgacaggttt acatcaccgc gaaccgggaa tggttggcgc
     8521 gggattaacg gacaagcgcg cctggctgga actgatagcc gatggtcatc atgtgcatcc
     8581 ggcggcgatg tcgctgtgtt gttgctgtgc aaaagagaga atcgtgctga tcaccgacgc
     8641 gatgcaggca gccgggatgc cggatggtcg ctatacgtta tgtggcgaag aagtgcagat
     8701 gcacggtggc gttgtccgta ccgcgtccgg tgggctggcg ggcagtacgc tgtctgttga
     8761 tgcggcagtg cgcaacatgg tcgagttgac gggcgtaacg cctgcggaag ccattcatat
     8821 ggcatcgctg catccggcgc gaatgctggg tgttgatggt gttctgggat cgcttaaacc
     8881 gggcaaacgc gccagcatcg ttgcgctgga tagcgggctg catgtgcaac aaatctggat
     8941 tcagagtcaa ttagcttcgt tttgatattt tgctccttta ttgggccttc acttcccccg
     9001 taaggccttt ctttttcttt cgttttgatc tgtgcagcgg tgtcggatgc gacgctaacg
     9061 cgtcttatgc gacctacggt acgccgcatc cagcaatcgg tgaacatagc gacgtcatta
     9121 cgcaattctt taatctcccc ttgtcttttc tttcaatgat cacagatttc gttttatttc
     9181 ctttctctcc attgaacttt cggtttcttt tctatagatt ttaatcaacg aaagacatca
     9241 ccaagtgaaa tgaaacgaaa ggcaagtgaa agcgacaacg cccgacgtca agttcatcag
     9301 actaaggatt gagttatgcc agaaaattac acccctgctg ccgccgcaac cggtacatgg
     9361 actgaagaag agatccgcca tcagcctcgc gcatggatcc gttcactcac caacatcgac
     9421 gcgctacgtt ctgcgctcaa taacttcctt gaaccgttac tgcgcaaaga gaatctgcgg
     9481 atcatcctga ccggagccgg aacctcggca tttatcggtg acatcatcgc gccgtggctc
     9541 gccagccata ccggtaaaaa cttcagcgcc gtaccgacca ccgatctggt caccaatccg
     9601 atggactacc tgaacccagc tcatccgctg ctgttgatct ccttcggtcg atccggcaac
     9661 agcccggaaa gcgtcgctgc cgtggaactg gcaaatcaat ttgtaccaga atgctatcac
     9721 ctgccgatca cctgcaacga agcgggcgct ctttaccaaa acgcgatcaa cagcgataac
     9781 gcgtttgccc tgctgatgcc cgcagaaacg cacgatcgcg gctttgcgat gaccagcagc
     9841 attaccacca tgatggccag ctgcctcgcg gttttcgcac ctgagacgat caacagccaa
     9901 accttccgcg acgtggcgga tcgttgccag gcgatcctga cctcactggg cgatttcagc
     9961 gaaggtgtgt ttggttacgc accgtggaaa cggatcgttt atctcggcag cggtggctta
    10021 cagggcgcag cacgcgagtc ggcgctgaaa gtgctggaac tgactgcggg taaactggcg
    10081 gccttctatg attccccgac cggattccgt catggcccga aatcactggt cgataacgaa
    10141 acgctggtgg tggtatttgt ctcaagccac ccttacaccc gtcagtatga tcttgatctg
    10201 ctggctgaac tccgccgtga caaccaggca atgcgcgtaa tcgccatcgc cgcggaaagc
    10261 accgacatcg tcgctgccgg tccacatatt atcctgccgc cgtcacgtca ctttatcgac
    10321 gttgagcagg cattttgctt cctgatgtac gcccagacgt ttgcactgat gcagtcgctg
    10381 cacatgggca atacgccgga taccccatca gccagcggca ccgttaaccg cgtggtgcaa
    10441 ggcgtaatca ttcatccgtg gcaggcataa gaggatcgca ttatgagcat tatctccact
    10501 aaatatctgt tacaggacgc ccaggccaat ggctacgcgg tgcctgcttt taacattcat
    10561 aacgccgaga cgatccaggc gatcctcgaa gtgtgcagtg aaatgcgatc gccggtgatc
    10621 ctcgccggaa cgccggggac ctttaaacat atcgcgctgg aagagatcta cgccctgtgc
    10681 agcgcctatt ccacaaccta caacatgcca ctggcgctgc atctcgacca ccacgaatcg
    10741 ctggatgata ttcgccgtaa agtccacgca ggtgtgcgca gtgcaatgat cgacggcagc
    10801 cacttcccgt ttgccgagaa cgtgaagctg gtgaaatcgg ttgttgactt ctgccactca
    10861 caggattgca gcgtggaagc agaactgggc cgcctgggcg gtgttgaaga tgacatgagc
    10921 gttgacgccg aaagtgcatt cctgaccgat ccacaagaag ctaaacgctt tgtcgaactg
    10981 actggcgtcg acagcctggc ggtagcgatt ggtacggcgc acggcttata cagcaaaacg
    11041 ccgaagattg atttccagcg gctggcggaa attcgtgaag tggtggatgt tcctctggtg
    11101 ctacatggtg ccagcgatgt tccggatgaa tttgtccgtc gcactattga acttggcgtc
    11161 acaaaagtga acgttgccac agaattaaaa atagccttcg ctggcgcggt taaagcctgg
    11221 tttgcggaaa atccgcaggg taatgatcct cgttattata tgcgcgtcgg aatggatgcg
    11281 atgaaagaag ttgtcagaaa taaaattaat gtctgtggtt cagcgaatcg aatttcagca
    11341 taatcattag ttttttatga tttatcccaa tgtacttccc gat
//
LOCUS       DAZKHF020000142        11379 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2201S31241
            SAMN43267800-rid25106413.denovo.143, whole genome shotgun sequence.
ACCESSION   DAZKHF020000142 DAZKHF020000000
VERSION     DAZKHF020000142.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267800
            Sequence Read Archive: SRR30307263
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 11379)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11379)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 11379)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350581.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350581.2
            Genome Coverage       :: 151x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:21:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,652
            CDSs (total)                      :: 5,557
            Genes (coding)                    :: 5,320
            CDSs (with protein)               :: 5,320
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 237
            CDSs (without protein)            :: 237
            Pseudo Genes (ambiguous residues) :: 0 of 237
            Pseudo Genes (frameshifted)       :: 102 of 237
            Pseudo Genes (incomplete)         :: 130 of 237
            Pseudo Genes (internal stop)      :: 52 of 237
            Pseudo Genes (multiple problems)  :: 41 of 237
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11379
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267800-rid25106413.denovo.143"
                     /serotype="O 26:H11"
                     /isolate="2201S31241"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-01"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            242..541
                     /gene="yoaC"
                     /locus_tag="ACHOSC_RS15300"
                     /old_locus_tag="pgaptmp_003060"
     CDS             242..541
                     /gene="yoaC"
                     /locus_tag="ACHOSC_RS15300"
                     /old_locus_tag="pgaptmp_003060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416324.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1889 family protein"
                     /protein_id="HGU4548957.1"
                     /translation="MPAVIDKALDFIGAMDVSAPTPSSMNESTAKGIFKYLKELGVPA
                     SAADITARADQEGWNPGFTEKMVGWAKKMETGERSVIKNPEYFSTYMQEELKALV"
     gene            complement(661..840)
                     /gene="yoaH"
                     /locus_tag="ACHOSC_RS15305"
                     /old_locus_tag="pgaptmp_003061"
     CDS             complement(661..840)
                     /gene="yoaH"
                     /locus_tag="ACHOSC_RS15305"
                     /old_locus_tag="pgaptmp_003061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YoaH family protein"
                     /protein_id="HGU4548958.1"
                     /translation="MFAGLPSLTHEQQQKAVERIQELMAQGMSSGQAIALVAEELRAN
                     HSGERIVARFEDEDE"
     gene            914..2275
                     /gene="pabB"
                     /locus_tag="ACHOSC_RS15310"
                     /old_locus_tag="pgaptmp_003062"
     CDS             914..2275
                     /gene="pabB"
                     /locus_tag="ACHOSC_RS15310"
                     /old_locus_tag="pgaptmp_003062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009396 - folic acid-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminodeoxychorismate synthase component 1"
                     /protein_id="HGU4548959.1"
                     /translation="MKTLSPAVITLPWRQDAAEFYFSRLSHLPWAMLLHSGYADHPYS
                     RFDIVVADPICTLTTFGKETVVSESEKRTTTTDDPLQVLQQVLDRADIRPTHNEDLPF
                     QGGALGLFGYDLGRRFESLPEIAEQDIVLPDMAVGIYDWALIVDHQRHTVSLLSHNDV
                     NARRAWLESQQFSPQEDFTLTSDWQSNMTREQYGEKFRQVQEYLHSGDCYQVNLAQRF
                     HATYSGDEWQAFLQLNQANRAPFSAFLRLEQGAILSLSPERFILCDNSEIQTRPIKGT
                     LPRLPDPQEDSKQAEKLANSAKDRAENLMIVDLMRNDIGRVAVAGSVKVPELFVVEPF
                     PAVHHLVSTITARLPEQLHASDLLRAAFPGGSITGAPKVRAMEIIDELEPQRRNAWCG
                     SIGYLSFCGNMDTSITIRTLTAINGQIYCSAGGGIVADSQEEAEYQETFDKVNKILRQ
                     LEK"
     gene            2279..2857
                     /gene="nudL"
                     /locus_tag="ACHOSC_RS15315"
                     /old_locus_tag="pgaptmp_003063"
     CDS             2279..2857
                     /gene="nudL"
                     /locus_tag="ACHOSC_RS15315"
                     /old_locus_tag="pgaptmp_003063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0010945 - coenzyme A diphosphatase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA pyrophosphatase"
                     /protein_id="HGU4548960.1"
                     /translation="MEYRSLTLDDFLSRFQLLRPQINRETLNHRQAAVLIPIVRRPQP
                     GLLLTQRSIHLRKHAGQVAFPGGAVDDTDASVIAAALREAEEEVAIPPSAVEVIGVLP
                     PVDSVTGYQVTPVVGIIPPDLPYRASEDEVSAVFEMPLAQALHLGRYHPLDIYRRGDS
                     HRVWLSWYEQYFVWGMTAGIIRELALQIGVKP"
     gene            3041..4405
                     /gene="sdaA"
                     /locus_tag="ACHOSC_RS15320"
                     /old_locus_tag="pgaptmp_003064"
     CDS             3041..4405
                     /gene="sdaA"
                     /locus_tag="ACHOSC_RS15320"
                     /old_locus_tag="pgaptmp_003064"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase"
                     /protein_id="HGU4548961.1"
                     /translation="MISLFDMFKVGIGPSSSHTVGPMKAGKQFVDDLVEKGLLDSVTR
                     VAVDVYGSLSLTGKGHHTDIAIIMGLAGNEPATVDIDSIPGFIRDVEERERLLLAQGR
                     HEVDFPRDNGMRFHNGNLPLHENGMQIHAYNGDEVVYSKTYYSIGGGFIVDEEHFGQD
                     AANEVSVPYPFKSATELLAYCNETGYSLSGLAMQNELALHSKKEIDEYFAHVWQTMQA
                     CIDRGMNTEGVLPGPLRVPRRASALRRMLVSSDKLSNDPMNVIDWVNMFALAVNEENA
                     AGGRVVTAPTNGACGIVPAVLAYYDHFIESVSPDIYTRYFMAAGAIGALYKMNASISG
                     AEVGCQGEVGVACSMAAAGLAELLGGSPEQVCVAAEIGMEHNLGLTCDPVAGQVQVPC
                     IERNAIASVKAINAARMALRRTSAPRVSLDKVIETMYETGKDMNAKYRETSRGGLAIK
                     VQCD"
     gene            4536..6134
                     /gene="pdeD"
                     /locus_tag="ACHOSC_RS15325"
                     /old_locus_tag="pgaptmp_003065"
     CDS             4536..6134
                     /gene="pdeD"
                     /locus_tag="ACHOSC_RS15325"
                     /old_locus_tag="pgaptmp_003065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416329.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HGU4548962.1"
                     /translation="MQKAQRIIKTYRRNRMIVCTICALVTLASTLSVRFISQRNLNQQ
                     RVVQFANHAVEELDKVLLPLQAGSEVLLPLIGLPCSVAHLPLRKQAAKLQTVRSIGLV
                     QDGTLYCSSIFGYRNVPVVDILAELPAPQPLLRLTIDRALIKGSPVLIQWTPAAGSSN
                     AGVMEMINIDLLTAMLLEPQLPQISSASLTVDKRHLLYGNGLVDSLPQPEDNENYQVS
                     SQRFPFTINVNGPGATALAWHYLPTQLPLAVLLSLLVGYIAWLATAYRMSFSREINLG
                     LAQHEFELFCQPLLNARSQQCIGVEILLRWNNPRQGWISPDVFIPIAEEHHLIVPLTR
                     YVMAETIRQRHVFPMSSQFHVGINVAPSHFRRGVLIKDLNQYWFSAHPIQQLILEITE
                     RDALLDVDYRIARELHRKNVKLAIDDFGTGNSSFSWLETLRPDVLKIDKSFTAAIGSD
                     AVNSTVTDIIIALGQRLNIELVAEGVETQEQAKYLRRHGVHILQGYLYAQPMPLRDFP
                     KWLAGSQPPPARHNGHITPIMPLR"
     gene            complement(6138..7694)
                     /gene="yoaE"
                     /locus_tag="ACHOSC_RS15330"
                     /old_locus_tag="pgaptmp_003066"
     CDS             complement(6138..7694)
                     /gene="yoaE"
                     /locus_tag="ACHOSC_RS15330"
                     /old_locus_tag="pgaptmp_003066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CNNM family cation transport protein YoaE"
                     /protein_id="HGU4548963.1"
                     /translation="MEFLMDPSIWAGLLTLVVLEIVLGIDNLVFIAILADKLPPKQRD
                     KARLLGLSLALIMRLGLLSLISWMVTLTKPLFTVMDFSFSGRDLIMLFGGIFLLFKAT
                     TELHERLENRDHDSGHGKGYASFWVVVTQIVILDAVFSLDAVITAVGMVNHLPVMMAA
                     VVIAMAVMLLASKPLTRFVNQHPTVVVLCLSFLLMIGLSLVAEGFGFHIPKGYLYAAI
                     GFSIIIEVFNQIARRNFIRHQSTLPLRARTADAILRLMGGKRQANVQHDADNPMPMPI
                     PEGAFAEEERYMINGVLTLASRSLRGIMTPRGEISWVDANLGVDEIREQLLSSPHSLF
                     PVCRGELDEIIGIVRAKELLVALEEGVDVAAIASASPAIIVPETLDPINLLGVLRRAR
                     GSFVIVTNEFGVVQGLVTPLDVLEAIAGEFPDADETPEIITDGDGWLVKGGTDLHALQ
                     QALDVEHLADDDDIATVAGLVISANGHIPRVGDVIDVGPLHITIIEANDYRVDLVRIV
                     KEQPAHDEDE"
     gene            complement(7682..7840)
                     /locus_tag="ACHOSC_RS15335"
                     /old_locus_tag="pgaptmp_003067"
     CDS             complement(7682..7840)
                     /locus_tag="ACHOSC_RS15335"
                     /old_locus_tag="pgaptmp_003067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518795.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4548964.1"
                     /translation="MDRHRCHFSIRPFNAYLSGSLCRTFRLHFVVTPALFLASNSYSL
                     SRSLSWNS"
     gene            8157..9128
                     /gene="manX"
                     /locus_tag="ACHOSC_RS15340"
                     /old_locus_tag="pgaptmp_003068"
     CDS             8157..9128
                     /gene="manX"
                     /locus_tag="ACHOSC_RS15340"
                     /old_locus_tag="pgaptmp_003068"
                     /EC_number="2.7.1.191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose transporter subunit IIAB"
                     /protein_id="HGU4548965.1"
                     /translation="MTIAIVIGTHGWAAEQLLKTAEMLLGEQENVGWIDFVPGENAET
                     LIEKYNAQLAKLDTTKGVLFLVDTWGGSPFNAASRIVVDKEHYEVIAGVNIPMLVETL
                     MARDDDPSFDELVALAVETGREGVKALKAKPVEKAAQAPAAAAPKAAPTPAKPMGPND
                     YMVIGLARIDDRLIHGQVATRWTKETNVSRIIVVSDEVAADTVRKTLLTQVAPPGVTA
                     HVVDVAKMIRVYNNPKYAGERVMLLFTNPTDVERLVEGGVKITSVNVGGMAFRQGKTQ
                     VNNAVSVDEKDIEAFKKLNARGIELEVRKVSTDPKLKMMDLISKIDK"
     gene            9191..9991
                     /gene="manY"
                     /locus_tag="ACHOSC_RS15345"
                     /old_locus_tag="pgaptmp_003069"
     CDS             9191..9991
                     /gene="manY"
                     /locus_tag="ACHOSC_RS15345"
                     /old_locus_tag="pgaptmp_003069"
                     /EC_number="2.7.1.199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002910918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose transporter subunit IIC"
                     /protein_id="HGU4548966.1"
                     /translation="MEITTLQIVLVFIVACIAGMGSILDEFQFHRPLIACTLVGIVLG
                     DMKTGIIIGGTLEMIALGWMNIGAAVAPDAALASIISTILVIAGHQSIGAGIALAIPL
                     AAAGQVLTIIVRTITVAFQHAADKAADNGNLTAISWIHVSSLFLQAMRVAIPAVIVAL
                     SVGTSEVQNMLNAIPEVVTNGLNIAGGMIVVVGYAMVINMMRAGYLMPFFYLGFVTAA
                     FTNFNLVALGVIGTVMAVLYIQLSPKYNRVAGAPAQAAGNNDLDNELD"
     gene            10004..10855
                     /gene="manZ"
                     /locus_tag="ACHOSC_RS15350"
                     /old_locus_tag="pgaptmp_003070"
     CDS             10004..10855
                     /gene="manZ"
                     /locus_tag="ACHOSC_RS15350"
                     /old_locus_tag="pgaptmp_003070"
                     /EC_number="2.7.1.199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020804907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose transporter subunit IID"
                     /protein_id="HGU4548967.1"
                     /translation="MVDTTQTTTEKKLTQSDIRGVFLRSNLFQGSWNFERMQALGFCF
                     SMVPAIRRLYPENNEARKQAIRRHLEFFNTQPFVAAPILGVTLALEEQRANGAEIDDG
                     AINGIKVGLMGPLAGVGDPIFWGTVRPVFAALGAGIAMSGSLLGPLLFFILFNLVRLA
                     TRYYGVAYGYSKGIDIVKDMGGGFLQKLTEGASILGLFVMGALVNKWTHVNIPLVVSR
                     ITDQTGKEHVTTVQTILDQLMPGLVPLLLTFACMWLLRKKVNPLWIIVGFFVIGIAGY
                     ACGLLGL"
     gene            10910..11368
                     /gene="yobD"
                     /locus_tag="ACHOSC_RS15355"
                     /old_locus_tag="pgaptmp_003071"
     CDS             10910..11368
                     /gene="yobD"
                     /locus_tag="ACHOSC_RS15355"
                     /old_locus_tag="pgaptmp_003071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF986 family protein"
                     /protein_id="HGU4548968.1"
                     /translation="MTITDLVLILFIAALLAFAIYDQFIMPRRNGPTLLAIPLLRRGR
                     IDSVIFVGLIVILIYNNVTNHGALITTWLLSALALMGFYIFWIRVPKIIFKQKGFFFA
                     NVWIEYSRIKAMNLSEDGVLVMQLEQRRLLIRVRNIDDLEKIYKLLVSTQ"
BASE COUNT         2787 a         2871 c         2865 g         2856 t
ORIGIN      
        1 ttcgccctct gactagcaac tctggttttc tttcttagct gaataatctg gttatagcgt
       61 gaggactgac cgttaaattt ctccatctta ctggataaca caccatgagc tgctaccttt
      121 aaaacacctg aatgtcaaca ggttaactcg tgccatatcg ttataatccc tttgatatta
      181 gatgcaaatt aaggtcatat atagtcttat cgataacatg gttaatttta aggataagaa
      241 tatgcctgct gtaatagata aagccctgga tttcattggt gccatggatg tatcagcgcc
      301 aacaccaagt tcgatgaatg aaagcacggc gaagggaatc tttaaatatt taaaagaact
      361 gggagtaccc gcgagtgccg ctgatattac ggcgcgagct gaccaggaag gctggaaccc
      421 tgggttcacg gaaaaaatgg ttggatgggc aaaaaaaatg gagacaggtg aacgctctgt
      481 gattaaaaat cctgaatact tttcaacata tatgcaggaa gaactaaaag cactggtctg
      541 agttaaattt atatcagcat aaatgggtca ggacgctttt aatcacatat aaaaagcgtc
      601 ctttttctca caatcaaatg atcatcggct tctataacga aatcgatacg gattagtgtg
      661 ttactcgtct tcatcctcaa aacgcgccac gattcgctcg ccggagtggt tggcacgcag
      721 ctcttctgcc accagcgcga ttgcctggcc gctgctcatc ccttgtgcca tcagttcctg
      781 gatccgctcg acagcttttt gctgctgttc atgggtgagt gaaggtaaac ctgcaaacat
      841 tgttaactcc tgctaaattg ttggcgctaa ttatttcatg ctacccggca catagccagt
      901 agagtcagga ctgatgaaga cgttatctcc cgctgtgatt actttaccct ggcgtcagga
      961 cgccgctgaa ttttatttct cccgcttaag ccacctgccg tgggcgatgc ttttacactc
     1021 cggctatgcc gatcatccgt atagccgctt tgatattgtg gtcgccgatc cgatttgcac
     1081 tttaaccact ttcggtaaag aaaccgttgt tagtgaaagc gaaaaacgca caacgaccac
     1141 tgatgacccg ctacaggtgc tccagcaggt gctggatcgc gcagacattc gcccaacgca
     1201 taacgaagat ttgccatttc agggcggcgc actggggttg tttggctacg atctgggccg
     1261 ccgttttgag tcactgccag aaattgcgga acaagatatc gttctgccgg atatggcagt
     1321 gggtatctac gattgggcgc tcattgtcga ccaccagcgt catacagttt ctttgctgag
     1381 tcataatgat gtcaatgccc gtcgggcctg gctggaaagc cagcaattct cgccgcagga
     1441 agatttcacg ctcacttccg actggcaatc caatatgacc cgcgagcagt acggcgaaaa
     1501 atttcgccag gtacaggaat atctgcacag cggtgattgc tatcaggtga atctcgccca
     1561 gcgttttcat gcgacctatt ctggcgatga atggcaggca ttccttcagc ttaatcaggc
     1621 caaccgcgcg ccatttagcg cttttttacg tcttgaacag ggtgcaattt taagcctttc
     1681 gccagagcgg tttattcttt gtgataatag tgaaatccag acccgcccga ttaaaggcac
     1741 gctaccacgc ctgcccgatc ctcaggaaga tagcaaacaa gcagaaaaac tggcgaactc
     1801 agcgaaagat cgtgccgaaa atctgatgat tgtcgattta atgcgtaatg atatcggtcg
     1861 tgttgccgta gccggttcgg taaaagtacc agagctcttc gtggtggaac ccttccctgc
     1921 cgtgcatcat ctggtcagca ctataacggc gcgactacca gaacagttac acgccagcga
     1981 tctgctgcgc gcagcttttc ctggtggctc aataaccggg gctccgaaag tacgggctat
     2041 ggaaattatc gacgaactgg aaccgcagcg acgtaatgcc tggtgcggca gcattggcta
     2101 tttgagcttt tgcggcaaca tggataccag cattactatc cgcacgctga ctgccattaa
     2161 cggacaaata tactgctctg cgggcggtgg aattgtcgcc gatagccagg aagaagcgga
     2221 atatcaggaa acttttgata aagttaataa gatattacgc caactggaga agtaagacgt
     2281 ggaataccgt agcctgacgc ttgatgattt tttatctcgc tttcaacttt tgcgcccgca
     2341 aattaaccgg gaaaccctaa atcatcgtca ggctgccgtg ttaatcccca tcgtccgtcg
     2401 accgcaaccg gggttgttgc tgactcagcg ttcaattcat ctgcgtaaac acgctggaca
     2461 agtggcattc cctggaggtg cagtcgatga cacggacgca tcagttatcg ccgccgcgct
     2521 gcgcgaagct gaagaagagg tcgctatacc gccttccgcc gttgaagtta tcggcgtgct
     2581 gccgcccgtc gatagcgtaa ctggctatca ggtaacccca gtggtcggca ttatcccgcc
     2641 cgatctgccg tatcgcgcca gtgaagatga agtctcggcg gtgtttgaaa tgccgctcgc
     2701 ccaggcatta catctgggtc gttatcaccc tttagatatc taccgccgtg gcgattcaca
     2761 tcgggtatgg ctgtcctggt acgaacagta ttttgtatgg ggaatgaccg caggcataat
     2821 tcgtgagctg gcgctgcaaa ttggtgtgaa accctgacta tacttatctt tacatctaca
     2881 aaacactact tgagacaatc atcgcaatat tagttaaatc gcggtttttg attagtttaa
     2941 ttcatgtgaa tagttaagcc agtcgccgcg ttccctctta cactatgcgc tgttattagt
     3001 tcgttactgg aagtccagtc accttgtcag gagtattatc gtgattagtc tattcgacat
     3061 gtttaaggtg gggattggtc cctcatcttc ccataccgta gggcctatga aggcgggtaa
     3121 acagttcgtc gatgatctgg tcgaaaaagg cttactggat agcgttactc gcgttgccgt
     3181 ggacgtttat ggttcactgt cgctgacggg taaaggccac cacaccgata tcgccattat
     3241 tatgggtctt gcaggtaacg aacctgccac cgtggatatc gacagtattc ccggttttat
     3301 tcgcgacgta gaagagcgcg aacgtctgct gctggcacag ggacggcatg aagtggattt
     3361 cccgcgcgac aacgggatgc gttttcataa cggcaacctg ccgctgcatg aaaacggtat
     3421 gcaaatccac gcctataacg gcgatgaagt cgtctacagc aaaacttatt attccatcgg
     3481 cggcggtttt atcgtcgatg aagaacactt tggtcaggat gctgccaacg aagtgagcgt
     3541 gccgtatccg ttcaaatctg ccaccgaact gctcgcgtac tgtaatgaaa ccggctattc
     3601 gctgtctggt ctcgctatgc agaacgaact ggcgctgcac agcaagaaag agatcgacga
     3661 gtatttcgcg catgtctggc aaaccatgca ggcatgtatc gatcgcggga tgaacaccga
     3721 aggtgtactg ccaggcccgc tgcgcgtgcc acgtcgtgcg tctgccctgc gccggatgtt
     3781 agtttccagc gataaactgt ctaacgatcc gatgaatgtc attgactggg taaacatgtt
     3841 tgcgctggca gttaacgaag aaaacgccgc cggtggtcgt gtggtaactg cgccaaccaa
     3901 cggtgcctgc ggtatcgttc cggcagtgct ggcttactat gaccacttta ttgaatcggt
     3961 cagcccggac atctataccc gttactttat ggcagcgggc gcgattggtg cattgtataa
     4021 aatgaacgcc tctatttccg gtgcggaagt tggttgccag ggcgaagtgg gtgttgcctg
     4081 ttcaatggct gctgcgggtc ttgcagaact gctgggcggt agcccggaac aggtttgcgt
     4141 ggcggcggaa attggcatgg aacacaacct tggtttaacc tgcgacccgg ttgcagggca
     4201 ggttcaggtg ccgtgcattg agcgtaatgc cattgcctct gtgaaggcga ttaacgccgc
     4261 acggatggct ctgcgccgca ccagtgcacc gcgcgtctcg ctggataagg tcatcgaaac
     4321 gatgtacgaa accggtaagg acatgaacgc caaataccgc gaaacctcac gcggtggtct
     4381 ggcaatcaaa gtccagtgtg actaatactt cttactcgcc catctgcaac ggatgggcga
     4441 atttataccc gctttctcgt ctgctgtaat attccccact acacttccac tgttgcgtca
     4501 ggcgtttgtc gccatacgct tacagggtgg cccgcatgca aaaagcacaa cggatcatta
     4561 aaacctatcg ccgtaatcga atgattgttt gtacgatttg cgcactcgtt acgctcgctt
     4621 cgaccctgag cgtgcgattt atttcacagc gtaacttaaa tcaacaacgg gtagtacaat
     4681 tcgccaatca cgctgtagag gaattagata aagtactgct tcccctacag gcaggtagcg
     4741 aagtcttgct tccgctgatt ggtctgccct gctctgtcgc ccatttgcca ttacgtaaac
     4801 aggcggcaaa actccaaact gtgcgatcca ttggcctggt gcaagacggc acactctatt
     4861 gctccagcat ttttggttat cgcaatgtgc ccgtcgtgga cattctggct gaacttcctg
     4921 caccgcaacc acttttacgc ctgacgatcg accgtgccct gattaaaggc agtccggttt
     4981 tgattcaatg gacgcctgca gcgggcagta gcaatgctgg ggtcatggag atgattaaca
     5041 tcgacttact gacggcaatg ctccttgagc cacaactgcc gcaaatcagt agcgccagcc
     5101 tgacggtgga caaacggcat ttgctctatg gtaatgggct ggtagattcc cttccgcaac
     5161 ctgaagacaa tgaaaactat caggtttctt cgcaacgctt tccttttacc attaacgtta
     5221 atggtccggg ggctacggcg ctggcatggc actatcttcc aacacaatta ccgctggcgg
     5281 tgctgctaag tttactggtg ggctacatcg cctggctggc gaccgcttac cggatgagct
     5341 tttcccgcga aatcaatctg ggcctggcgc aacatgagtt cgaattgttc tgtcagcctt
     5401 tgcttaatgc gcgcagccag caatgtattg gtgtagagat tttgctgcgc tggaataatc
     5461 cgcgtcaggg ctggatttca ccggatgtgt ttattcctat cgcggaagaa catcatttaa
     5521 ttgtgccact gacccgctat gtgatggcag aaaccattcg tcagcgccat gttttcccga
     5581 tgagtagtca gtttcatgtt ggcattaacg tcgcacccag ccattttcgc cgtggtgtgc
     5641 tgataaaaga tctcaatcag tactggttta gcgctcaccc gattcagcaa ctgatcctcg
     5701 aaatcaccga acgcgatgcc ttactggatg ttgattatcg gattgcccgc gagctacatc
     5761 gtaaaaacgt caaactggcg attgatgact tcggcaccgg caacagttca ttttcctggc
     5821 ttgaaacatt acgtcctgac gtgctgaaaa ttgataagtc atttaccgca gctataggtt
     5881 ctgacgcggt taactcgacg gtgaccgata tcatcatcgc gctggggcaa agactgaata
     5941 ttgaactggt ggcggagggc gtggaaacgc aagaacaggc gaagtatttg cgccgtcatg
     6001 gcgtgcatat tttgcaaggg tatttgtacg cacagccgat gccgctacgt gattttccca
     6061 aatggctggc gggcagccaa ccgccgcccg cccggcataa tggacatatc acgcccatta
     6121 tgccgttacg ttaatgctta ctcatcttca tcgtgcgccg gttgctcttt aacaatgcga
     6181 accagatcaa cacgataatc attggcttca atgatggtga tatgcagtgg ccctacatca
     6241 atcacatcgc ccacacgggg aatgtgacca tttgccgaga tcacgaggcc cgcgaccgtc
     6301 gcgatatcat cgtcatcggc aaggtgctca acatcaagcg cctgctgcaa ggcatgcaaa
     6361 tctgtaccgc cttttaccag ccagccgtca ccatcagtaa tgatttccgg cgtttcgtca
     6421 gcgtccggga attcacccgc aatggcttcc agcacatcca gcggcgtgac cagaccttgt
     6481 accacaccaa actcgttggt cacgataaca aagctcccgc gagcacgacg cagcacgccc
     6541 aacaggttga tcggatcgag ggtttccggg acgataatcg ccggagacgc cgaagcaatc
     6601 gccgccacat caacgccctc ttccagcgcc accagcagtt ctttagcacg tacaataccg
     6661 atgatttcat ccagttcacc gcgacatacc gggaacagac tgtgcggtga agagagcagt
     6721 tgctcgcgga tttcatcgac cccgagatta gcgtcaaccc agcttatttc accgcgcggc
     6781 gtcatgatcc cgcgcagaga acgcgacgcc agcgtcagta cgccgttaat catgtaacgt
     6841 tcttcttcgg caaatgcacc ttccgggatc ggcatcggca tcgggttatc ggcatcgtgc
     6901 tgaacattgg cctgacgttt cccgcccatc aaacgcagga tggcatcggc agtacgcgct
     6961 cgcagcggca aagtcgactg gtggcgaata aagttgcgac gcgcaatctg gttaaacact
     7021 tcgatgatga tcgagaagcc aatcgcggca tacaggtaac ctttcggaat gtggaaaccg
     7081 aaaccttctg ccaccagact cagaccaatc attaacagga agctcagaca gagcaccacc
     7141 accgtggggt gctggttaac gaatcgcgtc agcggtttgg atgccagcaa cataaccgcc
     7201 atcgcaatca ctaccgccgc catcatcacc ggcagatggt taaccatccc tactgcagta
     7261 attaccgcat ccaacgagaa gacggcgtca aggatgacaa tctgtgtgac gaccacccag
     7321 aaactggcgt agcctttacc gtggccggaa tcatgatcgc ggttttccag ccgttcatgc
     7381 agttcggttg ttgctttgaa cagcaagaat atccccccga acaacataat caggtcgcgt
     7441 ccggagaagg agaaatccat gacggtaaat agcggtttgg tcagcgtgac catccatgaa
     7501 atcagcgaca gcagccccag acgcataatc agcgccagtg ataaccccag caaacgcgct
     7561 ttatcgcgtt gttttggcgg cagtttgtca gcaagaatgg cgatgaagac caggttatcg
     7621 atacccagca caatttcgag aacaacaagc gtgagtagcc ccgcccaaat tgaggggtcc
     7681 attaagaatt ccatgacaag ctcctgctta aggaatagct attcgacgcc agaaataatg
     7741 caggcgtaac gacaaaatgc aaacgaaagg tgcggcatag agagccagaa aggtaggcgt
     7801 taaaaggcct gatgctgaaa tgacatcggt gacgatccat actgcgggct actgccctat
     7861 actccatggt tgttaaacgg gagttaaaca tatcagagac gcctctgatt tggcaaagat
     7921 ttaccttcct ttgcaaacga atgtgacaag gatattttac ctttcgaaat ttctgctaat
     7981 cgaaagttaa attacggatc ttcatcacat aaaataattt tttcgatatc taaaataaat
     8041 cgcgaaacgc aggggttttt ggttgtagcc cttatctgaa tcgattcgat tgtggacgac
     8101 gattcaaaaa tacatctggc acgttgaggt gttaacgata ataaaggagg tagcaagtga
     8161 ccattgctat tgttataggc acacatggtt gggctgcaga gcagttgctt aaaacggcag
     8221 aaatgctgtt aggcgagcag gaaaacgtcg gctggatcga tttcgttcca ggtgaaaatg
     8281 ccgaaacgct gattgaaaag tacaacgctc agttggcaaa actcgacacc actaaaggcg
     8341 tgctgtttct cgttgataca tggggaggca gcccgttcaa tgctgccagc cgcattgtcg
     8401 tcgacaaaga gcattatgaa gtcattgcag gcgttaacat tccaatgctc gtggaaacgt
     8461 taatggcccg tgatgatgac ccaagctttg atgaactggt ggcactggca gtagaaacag
     8521 gccgtgaagg cgtgaaagct ctgaaagcca aaccggttga aaaagccgcg caagcacccg
     8581 ctgccgcagc accaaaagcg gctccaactc cggcaaaacc aatggggcca aacgactaca
     8641 tggttattgg ccttgcgcgt atcgacgacc gtctgattca cggtcaggtc gccacccgct
     8701 ggaccaaaga aaccaatgtc tcccgtatta ttgttgttag tgatgaagtg gctgcggata
     8761 ccgttcgtaa gacactgctc acccaggttg cacctccggg cgtaacagca cacgtagttg
     8821 atgttgccaa aatgattcgc gtctacaaca acccgaaata tgctggcgaa cgcgtaatgc
     8881 tgttatttac caacccaaca gatgtagagc gtctcgttga aggcggcgtg aaaatcacct
     8941 ctgttaacgt cggtggtatg gcattccgtc agggtaaaac ccaggtgaat aacgcggttt
     9001 cggttgatga aaaagatatc gaggcgttca agaaactgaa tgcgcgcggt attgagctgg
     9061 aagtccgtaa ggtttccacc gatccgaaac tgaaaatgat ggatctgatc agcaaaatcg
     9121 ataagtaacg tattgtgttg attatcactc agttttcaca cttaagtctt acgtaaacag
     9181 gagaagtaca atggagatta ccactcttca aattgtgctg gtatttatcg tagcctgtat
     9241 cgcaggtatg ggatcaatcc tcgatgaatt tcagtttcac cgtccgctaa tcgcgtgtac
     9301 cctggtgggt atcgttcttg gggatatgaa aaccggtatt attatcggtg gtacgctgga
     9361 aatgatcgcg ctgggctgga tgaacatcgg tgctgcagtt gcgcctgacg ccgctctggc
     9421 ttctatcatt tctaccattc tggttatcgc aggtcatcag agcattggtg caggtatcgc
     9481 actggcaatc cctctggccg ctgcgggcca ggtactgacc atcatcgttc gtactattac
     9541 cgttgctttc cagcacgctg cggataaggc tgctgataac ggcaacctga cagcgatttc
     9601 ctggatccac gtttcttctc tgttcctgca agcaatgcgt gtggctattc cggccgtcat
     9661 cgttgcgctg tctgttggta ccagcgaagt acagaacatg ctgaatgcga ttccggaagt
     9721 ggtgaccaat ggtctgaata tcgccggtgg catgatcgtg gtggttggtt atgcgatggt
     9781 tatcaacatg atgcgtgctg gctacctgat gccgttcttc tacctcggct tcgtaaccgc
     9841 agcattcacc aactttaacc tggttgctct gggtgtgatt ggtactgtta tggcagtgct
     9901 ctacatccaa cttagcccga aatacaaccg cgtagccggt gcgcctgctc aggcagctgg
     9961 taacaacgat ctcgataacg aactggacta acaggtgagc gaaatggttg atacaactca
    10021 aactaccacc gagaaaaaac tcactcaaag tgatattcgt ggcgtcttcc tgcgttctaa
    10081 cctcttccag ggttcatgga acttcgaacg tatgcaggca ctgggtttct gcttctctat
    10141 ggtaccggca attcgtcgcc tctaccctga gaacaacgaa gctcgtaaac aagctattcg
    10201 ccgtcacctg gagttcttta acacccagcc gttcgtggct gcgccgattc tcggcgtaac
    10261 cctggcgctg gaagaacagc gtgctaatgg cgcagagatc gacgacggtg ctatcaacgg
    10321 tatcaaagtc ggtttgatgg ggccgctggc tggtgtaggc gacccgatct tctggggaac
    10381 cgtacgtccg gtatttgcag cactgggtgc cggtatcgcg atgagcggca gcctgttagg
    10441 tccgctgctg ttcttcatcc tgtttaacct ggtgcgtctg gcaacccgtt actacggcgt
    10501 agcgtatggt tactccaaag gtatcgatat cgttaaagat atgggtggtg gcttcctgca
    10561 aaaactgacg gaaggggcgt ctatcctcgg cctgtttgtc atgggggcat tggttaacaa
    10621 gtggacacat gtcaacatcc cgctggttgt ctctcgcatt actgaccaga cgggcaaaga
    10681 acacgttact actgtccaga ctattctgga ccagttaatg ccaggcctgg taccactgct
    10741 gctgaccttt gcttgtatgt ggctactgcg caaaaaagtt aacccgctgt ggatcatcgt
    10801 tggcttcttc gtcatcggta tcgctggtta cgcttgcggc ctgctgggac tgtaagactg
    10861 ttgtacacta ccggggcctt ttggccccgt ttttttatct ggaggattaa tgacaatcac
    10921 ggacctggta ctgattcttt tcatcgccgc actcctggcc ttcgcgatct acgatcagtt
    10981 catcatgccc cgccgtaacg gccccaccct gctggcaatt cctttgctcc ggcgtggtcg
    11041 catcgatagc gttatcttcg tcggattgat tgtcattctt atctataaca acgtcacgaa
    11101 tcatggtgcg ttaataacga catggttatt aagcgcactg gctctgatgg gtttttatat
    11161 attctggatc cgcgttccga agatcatctt taaacaaaaa ggttttttct tcgccaatgt
    11221 ctggattgaa tatagccgaa tcaaagcgat gaacttgtcg gaagatggcg tgttggtgat
    11281 gcaattagaa cagcgtcggc tgttaatccg cgttcgaaat atcgacgatc tggaaaaaat
    11341 ttataagctt ctcgtttcaa ctcaataagt tatgaattt
//
LOCUS       DAZKHF020000143        11369 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2201S31241
            SAMN43267800-rid25106413.denovo.144, whole genome shotgun sequence.
ACCESSION   DAZKHF020000143 DAZKHF020000000
VERSION     DAZKHF020000143.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267800
            Sequence Read Archive: SRR30307263
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 11369)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11369)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 11369)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350581.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350581.2
            Genome Coverage       :: 151x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:21:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,652
            CDSs (total)                      :: 5,557
            Genes (coding)                    :: 5,320
            CDSs (with protein)               :: 5,320
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 237
            CDSs (without protein)            :: 237
            Pseudo Genes (ambiguous residues) :: 0 of 237
            Pseudo Genes (frameshifted)       :: 102 of 237
            Pseudo Genes (incomplete)         :: 130 of 237
            Pseudo Genes (internal stop)      :: 52 of 237
            Pseudo Genes (multiple problems)  :: 41 of 237
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11369
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267800-rid25106413.denovo.144"
                     /serotype="O 26:H11"
                     /isolate="2201S31241"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-01"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            <1..424
                     /locus_tag="ACHOSC_RS15360"
                     /old_locus_tag="pgaptmp_003072"
     CDS             <1..424
                     /locus_tag="ACHOSC_RS15360"
                     /old_locus_tag="pgaptmp_003072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708149.1"
                     /note="catalyzes the formation of (1 R,6
                     R)-2-succinyl-6-hydroxy-2,4-cyclohexadiene-1-carboxylate
                     in menaquinone biosynthesis; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="2-succinyl-6-hydroxy-2,
                     4-cyclohexadiene-1-carboxylate synthase"
                     /protein_id="HGU4548969.1"
                     /translation="PGLQNAEQRAERQRSDRQWAQRFCTEPLTAVFADWYQQPVFASL
                     NDDQRRELVALRSNNNGATLAAMLEATSLAVQPDLRANLSARTFAFYYLCGERDSKFR
                     ALAAELAADCHVIPRAGHNAHRENPAGVIASLAQILRF"
     gene            439..1296
                     /gene="menB"
                     /locus_tag="ACHOSC_RS15365"
                     /old_locus_tag="pgaptmp_003073"
     CDS             439..1296
                     /gene="menB"
                     /locus_tag="ACHOSC_RS15365"
                     /old_locus_tag="pgaptmp_003073"
                     /EC_number="4.1.3.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001641973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008935 - 1,4-dihydroxy-2-naphthoyl-CoA
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009234 - menaquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-dihydroxy-2-naphthoyl-CoA synthase"
                     /protein_id="HGU4548970.1"
                     /translation="MIYPDEAMLYAPVEWHDCSEGFEDIRYEKSTDGIAKITINRPQV
                     RNAFRPLTVKEMIQALADARYDDNIGVIILTGAGDKAFCSGGDQKVRGDYGGYKDDSG
                     VHHLNVLDFQRQIRTCPKPVVAMVAGYSIGGGHVLHMMCDLTIAADNAIFGQTGPKVG
                     SFDGGWGASYMARIVGQKKAREIWFLCRQYDAKQALDMGLVNTVVPLADLEKETVRWC
                     REMLQNSPMALRCLKAALNADCDGQAGLQELAGNATMLFYMTEEGQEGRNAFNQKRQP
                     DFSKFKRNP"
     gene            1296..2258
                     /gene="menC"
                     /locus_tag="ACHOSC_RS15370"
                     /old_locus_tag="pgaptmp_003074"
     CDS             1296..2258
                     /gene="menC"
                     /locus_tag="ACHOSC_RS15370"
                     /old_locus_tag="pgaptmp_003074"
                     /EC_number="4.2.1.113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0016836 - hydro-lyase activity [Evidence
                     IEA];
                     GO_process: GO:0009234 - menaquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="o-succinylbenzoate synthase"
                     /protein_id="HGU4548971.1"
                     /translation="MRSAQVYRWQIPMDAGVVLRDRRLKTRDGLYVCLREGEREGWGE
                     ISPLPGFSQETWEEAQSVLLAWVNNWLAGDCELPQMPSVAFGVSCALAELADTLPQAA
                     NYRAAPLCNGDPDDLILKLADMPGEKVAKVKVGLYEAVRDGMVVNLLLEAIPDLHLRL
                     DANRAWTPLKGQQFAKYVNPDYRHRIAFLEEPCKTRDDSRAFARETGIAIAWDESLRE
                     PDFAFVAEEGVRAVVIKPTLTGSLEKVREQVQAAHALGLTAVISSSIESSLGLTQLAR
                     IAAWLTPDTIPGLDTLDLMQAQQVRRWPGSTLPVVEVDALERLL"
     gene            2255..3610
                     /gene="menE"
                     /locus_tag="ACHOSC_RS15375"
                     /old_locus_tag="pgaptmp_003075"
     CDS             2255..3610
                     /gene="menE"
                     /locus_tag="ACHOSC_RS15375"
                     /old_locus_tag="pgaptmp_003075"
                     /EC_number="6.2.1.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008756 - o-succinylbenzoate-CoA ligase
                     activity [Evidence IEA];
                     GO_process: GO:0009234 - menaquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="o-succinylbenzoate--CoA ligase"
                     /protein_id="HGU4548972.1"
                     /translation="MIFSDWPWRHWRQVRGEAIALRLNDEQLNWRELCARVDELASGF
                     AAQGVVEGSGVMLRAWNTPQTLLAWLALLQCGARVLPVNPQLPQPLLEELLPNLTLQF
                     ALVPDGENTFPALTSLHIQRVEGAHAATWQPTRLCSMTLTSGSTGLPKAAVHTYQAHL
                     ASAEGVLSLIPFGDHDDWLLSLPLFHVSGQGIMWRWLYAGARMTVRDKQPLEQMLAGC
                     THASLVPTQLWRLLVNRSSVSLKAVLLGGAAIPVELTEQAREQGIRCFCGYGLTEFAS
                     TVCAKEADGLADVGSPLPGREVKIVNNEVWLRAASMAEGYWRNGQLVSLVNDEGWYAT
                     RDRGEMHNGKLTIVGRLDNLFFSGGEGIQPEEVERVIAAHPAVLQVFIVPVADKEFGH
                     RPVAVVEYDQQTVDLGEWVKDKLARFQQPVRWLTLPPELKNGGIKISRQALKEWVQRQ
                     Q"
     gene            3720..3986
                     /gene="pmrD"
                     /locus_tag="ACHOSC_RS15380"
                     /old_locus_tag="pgaptmp_003076"
     CDS             3720..3986
                     /gene="pmrD"
                     /locus_tag="ACHOSC_RS15380"
                     /old_locus_tag="pgaptmp_003076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416762.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal transduction protein PmrD"
                     /protein_id="HGU4548973.1"
                     /translation="MEWLVKKSCCNKQDNRHVLMLCDAGGAIKMIAEVKSDFAVKVGD
                     LLSPLQNALYCINREKLHTVKVLSASSYSPDEWERQCTAAGKTQ"
     gene            complement(3980..4366)
                     /gene="arnF"
                     /locus_tag="ACHOSC_RS15385"
                     /old_locus_tag="pgaptmp_003077"
     CDS             complement(3980..4366)
                     /gene="arnF"
                     /locus_tag="ACHOSC_RS15385"
                     /old_locus_tag="pgaptmp_003077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311173.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-amino-4-deoxy-L-arabinose-phosphoundecaprenol
                     flippase subunit ArnF"
                     /protein_id="HGU4548974.1"
                     /translation="MGLIWGLFSVIIASVAQLSLGFAASHLPPMTHLWDFIAALLAFG
                     LDARILLLGLLGYLLSVFCWYKTLHKLALSKAYALLSMSYVLVWIASMVLPGWEGTFS
                     LKALLGVACIMSGLMLIFLPTTKQRY"
     gene            complement(4366..4701)
                     /gene="arnE"
                     /locus_tag="ACHOSC_RS15390"
                     /old_locus_tag="pgaptmp_003078"
     CDS             complement(4366..4701)
                     /gene="arnE"
                     /locus_tag="ACHOSC_RS15390"
                     /old_locus_tag="pgaptmp_003078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-amino-4-deoxy-L-arabinose-phosphoundecaprenol
                     flippase subunit ArnE"
                     /protein_id="HGU4548975.1"
                     /translation="MIWLTLVFASLLSVAGQLCQKQATCFVAINKRRKHIVLWLGLAL
                     ACLGLAMVLWLLVLQNVPVGIAYPMLSLNFVWVTLAAVKLWHEPVSPRHWCGVAFIIG
                     GIVILGSTV"
     gene            complement(4698..6350)
                     /gene="arnT"
                     /locus_tag="ACHOSC_RS15395"
                     /old_locus_tag="pgaptmp_003079"
     CDS             complement(4698..6350)
                     /gene="arnT"
                     /locus_tag="ACHOSC_RS15395"
                     /old_locus_tag="pgaptmp_003079"
                     /EC_number="2.4.2.43"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000030 - mannosyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016763 - pentosyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006493 - protein O-linked glycosylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipid IV(A)
                     4-amino-4-deoxy-L-arabinosyltransferase"
                     /protein_id="HGU4548976.1"
                     /translation="MKSVRYLIGLFAFIACYYLLPISTRLLWQPDETRYAEISREMLA
                     SGDWIVPHLLGLRYFEKPIAGYWINSIGQWLFGANNFGVRAGVIFATLLTAALVTWFT
                     LRLWRDKRLALLATVIYLSLFIVYAIGTYAVLDPFIAFWLVAGMCSFWLAMQAQTWKG
                     KSAGFLLLGITCGMGVMTKGFLALAVPVLSVLPWVATQKRWKDLFIYGWLAVISCVLT
                     VLPWGLAIAQREPDFWHYFFWVEHIQRFALDDAQHRAPFWYYVPVIIAGSLPWLGLLP
                     GALYTGWKNRKHSATVYLLSWTIMPLLFFSVAKGKLPTYILSCFASLAMLMAHYALLA
                     AKNNPLALRINGWINIAFGVTGIIATFVVSPWGPMNTPVWQTFESYKVFCAWSIFSLW
                     AFFGWYTLTNVEKTWPFAALCPLGLALLVGFSIPDRVMEGKHPQFFVEMTQESLQPSR
                     YILTDSVGVAAGLAWSLQRDDIIMYRQTGELKYGLNYPDAKGRFVSGDEFANWLNQHR
                     QEGIITLVLSVDRDEDINSLAIPPADAIDRQERLVLIQYRPK"
     gene            complement(6350..7240)
                     /gene="arnD"
                     /locus_tag="ACHOSC_RS15400"
                     /old_locus_tag="pgaptmp_003080"
     CDS             complement(6350..7240)
                     /gene="arnD"
                     /locus_tag="ACHOSC_RS15400"
                     /old_locus_tag="pgaptmp_003080"
                     /EC_number="3.5.1.n3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-deoxy-4-formamido-L-arabinose-
                     phosphoundecaprenol deformylase"
                     /protein_id="HGU4548977.1"
                     /translation="MTKVGLRIDVDTFRGTREGVPRLLEILSKHNIQASIFFSVGPDN
                     MGRHLWRLVKPQFLWKMLRSNAASLYGWDILLAGTAWPGKEIGHANADIIREAAKHHE
                     VGLHAWDHHAWQARSGNWDRQTMIDDIARGLRTLEEIIGQPVTCSAAAGWRADQKVIE
                     AKEAFHLRYNSDCRGAMPFRPLLESGNPGTAQIPVTLPTWDEVIGRDVKAEDFNGWLL
                     NRILRDKGTPVYTIHAEVEGCAYQHNFVDLLKRAAQEGVTFCPLSELLSETLPLGQVV
                     RGNIAGREGWLGCQQIAGSR"
     gene            complement(7237..9219)
                     /gene="arnA"
                     /locus_tag="ACHOSC_RS15405"
                     /old_locus_tag="pgaptmp_003081"
     CDS             complement(7237..9219)
                     /gene="arnA"
                     /locus_tag="ACHOSC_RS15405"
                     /old_locus_tag="pgaptmp_003081"
                     /EC_number="1.1.1.305"
                     /EC_number="2.1.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016742 - hydroxymethyl-, formyl- and
                     related transferase activity [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-4-amino-4-deoxy-L-arabinose
                     formyltransferase/UDP-glucuronic acid oxidase ArnA"
                     /protein_id="HGU4548978.1"
                     /translation="MKTVVFAYHDMGCLGIEALLAAGYEISAIFTHTDNPGEKAFYGS
                     VARLAAERGIPVYAPDDVNHPLWVERIAQLSPDVIFSFYYRHLICDEILQLAPAGAFN
                     LHGSLLPKYRGRAPLNWVLVNGETETGVTLHRMVKRADAGAIVAQLRIAIAPDDIAIT
                     LHHKLCHAARQLLEQTLPAIKHGNILEIAQRENEATCFGRRTPDDSFLEWHKPASVLH
                     NMVRAVADPWPGAFSYVGNQKFTVWSSRVHPHASKAQPGSVISVAPLLIACGDGALEI
                     VTGQAGDGITMQGSQLAQTLGLVQGSRLNSQPACTARRRTRVLILGVNGFIGNHLTER
                     LLREDHYEVYGLDIGSDAISRFLNHPHFHFVEGDISIHSEWIEYHVKKCDVVLPLVAI
                     ATPIEYTRNPLRVFELDFEENLRIIRYCVKYRKRIIFPSTSEVYGMCSDKYFDEDHSN
                     LIVGPVNKPRWIYSVSKQLLDRVIWAYGEKEGLQFTLFRPFNWMGPRLDNLNAARIGS
                     SRAITQLILNLVEGSPIKLIDGGKQKRCFTDIRDGIEALYRIIENAGNRCDGEIINIG
                     NPENEASIEELGEMLLASFEKHPLRHHFPPFAGFRVVESSSYYGKGYQDVEHRKPSIR
                     NAHRCLDWEPKIDMQETIDETLDFFLRTVDLTDKPS"
     gene            complement(9219..10187)
                     /gene="arnC"
                     /locus_tag="ACHOSC_RS15410"
                     /old_locus_tag="pgaptmp_003082"
     CDS             complement(9219..10187)
                     /gene="arnC"
                     /locus_tag="ACHOSC_RS15410"
                     /old_locus_tag="pgaptmp_003082"
                     /EC_number="2.4.2.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016780 - phosphotransferase activity, for
                     other substituted phosphate groups [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-phosphate
                     4-deoxy-4-formamido-L-arabinose transferase"
                     /protein_id="HGU4548979.1"
                     /translation="MFEIHPVKKVSVVIPVYNEQESLPELIRRTTTACESLGKEYEIL
                     LIDDGSSDNSAHMLVEASQAENSHIVSILLNRNYGQHSAIMAGFSHVTGDLIITLDAD
                     LQNPPEEIPRLVAKADEGYDVVGTVRQNRQDSWFRKTASKMINRLIQRTTGKAMGDYG
                     CMLRAYRRHIVDAMLHCHERSTFIPILANIFARRAIEIPVHHSEREFGESKYSFMRLI
                     NLMYDLVTCLTTTPLRMLSLLGSIIAIGGFSIAVLLVILRLTFGPQWAAEGVFMLFAV
                     LFTFIGAQFIGMGLLGEYIGRIYTDVRARPRYFVQQVIRPSSKENE"
     gene            complement(10191..11330)
                     /gene="arnB"
                     /locus_tag="ACHOSC_RS15415"
                     /old_locus_tag="pgaptmp_003083"
     CDS             complement(10191..11330)
                     /gene="arnB"
                     /locus_tag="ACHOSC_RS15415"
                     /old_locus_tag="pgaptmp_003083"
                     /EC_number="2.6.1.87"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_024188371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-4-amino-4-deoxy-L-arabinose
                     aminotransferase"
                     /protein_id="HGU4548980.1"
                     /translation="MSEFLPFSRPAMGVEELAAVKEVLESGWITTGPKNQALEQAFCQ
                     LTGNQHAIAVSSATAGMHITLMALEIGKGDEVITPSLTWVSTLNMISLLGATPVMVDV
                     DRDTPMVTPEAIESAITPRTKAIIPVHYAGAPADIDAIRAIGERYGIAVIEDAAHAVG
                     TYYKGRHIGAKGTTIFSFHAIKNITCAEGGLIVTDNENLARQLRMLKFHGLGVDAYDR
                     QTWGRAPQAEVLTPGYKYNLTDINAAIALTQLAKLEHLNTRRREIAQQYQQALAALPF
                     QPLSLPAWPHVHAWHLFIIRVDEQRCGISRDALMEALKERGIGTGLHFRAAHTQKYYR
                     ERFPTLSLPNTEWNSERICSLPLFPDMTTADADRVITALQQLAGQ"
BASE COUNT         2770 a         2956 c         3046 g         2597 t
ORIGIN      
        1 tccggggctg caaaatgctg aacaacgtgc ggaacgtcag cgttccgatc gccaatgggc
       61 gcagcgtttt tgcacagaac cgttaacggc ggtatttgcc gactggtatc aacagcctgt
      121 ttttgcctcg ctcaatgacg atcaacgccg ggagctggtg gcgctgcgca gcaacaataa
      181 tggcgcaacc cttgccgcca tgctggaggc gacttctctc gccgtccagc ctgatttacg
      241 tgctaacctt agcgcccgca catttgcgtt ttattattta tgtggtgaac gtgacagcaa
      301 attccgcgcc ctggcggcgg aactggctgc cgactgccat gtcattcctc gcgccggaca
      361 taacgcgcat cgggaaaatc ccgctggcgt aatcgcaagt ctggcgcaga tcttgcgttt
      421 ctgactaaag gacacaatat gatttatcct gatgaagcaa tgctttacgc accggttgaa
      481 tggcacgact gctccgaagg tttcgaggac attcgttatg aaaaatccac cgacggtatc
      541 gcaaaaatca ccattaatcg tccgcaggtg cgcaatgcct tccgtcctct gacggtaaaa
      601 gagatgatcc aggcactggc agatgcgcgt tatgacgaca atatcggcgt gatcattctg
      661 actggcgcag gcgataaagc gttctgctcc ggtggtgacc agaaagtgcg tggtgattac
      721 ggcggctata aagatgattc cggcgtacat cacttgaacg tgctggactt ccagcgtcag
      781 atccgtacct gtccgaaacc ggttgtcgcg atggtggctg gctactccat cggcggtggt
      841 cacgttctgc acatgatgtg cgacctgact atcgcagcag ataatgccat cttcggtcag
      901 actggcccga aagtcggttc cttcgacggc ggctggggcg cttcctacat ggctcgtatc
      961 gtcgggcaga aaaaagcgcg agaaatctgg ttcctgtgcc gtcagtacga cgcaaaacag
     1021 gcgctggata tgggccttgt caacaccgtg gtaccgctgg cggatctgga aaaagaaacc
     1081 gtccgttggt gccgcgaaat gctgcaaaac agcccgatgg cgctgcgctg cctgaaagct
     1141 gcgctgaacg ccgactgtga cgggcaggcg gggctgcagg agctggcggg caacgccacc
     1201 atgctgttct acatgacgga agaaggtcag gaaggtcgca acgctttcaa ccagaaacgt
     1261 cagcctgatt tcagcaaatt taaacggaat ccgtaatgcg tagcgcgcag gtataccgct
     1321 ggcagatccc catggacgcg ggggtggttc tgcgcgacag gcggttaaaa acccgcgacg
     1381 ggctgtacgt ttgcctgcgt gagggcgagc gcgaagggtg gggggagatc tccccactgc
     1441 cgggcttcag ccaggaaacc tgggaagagg cgcaaagcgt gctgcttgcc tgggtaaata
     1501 actggctggc aggcgattgc gagctaccgc agatgccttc cgttgccttt ggcgtaagct
     1561 gtgcattggc ggagctggca gatacgctcc cgcaggcggc caattaccgt gcggcaccac
     1621 tgtgtaatgg cgatcctgac gatctgatcc tcaaacttgc tgatatgcca ggcgagaaag
     1681 tggcgaaggt caaagtggga ttgtacgaag ccgtgcgcga cggcatggtg gttaatctgt
     1741 tgctggaggc aattccggat ctgcatttgc gtcttgacgc aaatcgcgcc tggacaccgc
     1801 tgaaaggcca gcagtttgcc aaatatgtta acccggatta tcgccaccgc atcgcgtttc
     1861 tcgaagagcc gtgcaaaacc cgcgatgatt cgcgagcgtt tgcccgtgaa accggcattg
     1921 ccattgcctg ggatgaaagc ttgcgcgagc cggattttgc ctttgtggct gaagagggcg
     1981 tgcgcgcggt agttatcaaa cccacgctca cgggcagtct ggagaaagta cgcgagcagg
     2041 tacaggcggc gcacgcgctg gggctgacgg cggtgatcag ttcttccatt gaatcgagct
     2101 taggcttaac gcaactggcg cggattgccg cctggttaac gccggacacc attccagggc
     2161 tggacacgct ggatctgatg caggcgcagc aggtacgtcg ctggccgggt agcacgctgc
     2221 ctgtcgtgga agttgatgca ctggagcggt tgttatgatc ttctctgact ggccgtggcg
     2281 tcactggcgg caagtgcggg gagaagccat cgccttacgt cttaatgacg agcaacttaa
     2341 ctggcgcgag ctttgtgctc gcgtcgatga attagcctca ggctttgcgg cgcagggcgt
     2401 tgttgagggc agcggcgtga tgttgcgggc gtggaatacg ccgcaaacgc tgctcgcctg
     2461 gctggcgtta ctgcaatgcg gggcgcgggt gttgcccgtg aaccctcagc tgccgcaacc
     2521 gttgcttgaa gaattgctgc ccaatctgac gctgcaattt gcgctggtgc cggatgggga
     2581 aaacacgttt ccggcgttaa cgtcgctgca cattcagcgg gttgaaggcg cacatgccgc
     2641 tacgtggcag ccgacgcgtc tgtgctcaat gacgttgacc tcgggttcta ccgggttgcc
     2701 gaaagccgct gtccatactt atcaggccca tcttgccagt gccgaagggg tgttatcgtt
     2761 gattccgttt ggcgatcacg atgactggtt gctctcttta ccgctgtttc acgtctccgg
     2821 tcagggaatt atgtggcgct ggttatacgc tggtgcgcgg atgacggtgc gtgataaaca
     2881 gccattggag caaatgctgg caggctgtac tcatgcttca ctggtgccaa cacaactctg
     2941 gcgtttgctg gttaaccgca gttccgtttc cctgaaagcg gtgttacttg gcggcgcggc
     3001 tatcccggtc gagttgacgg aacaggcgcg cgagcagggg attcgttgct tttgcggcta
     3061 tggtctgacc gagtttgcct ccacggtgtg tgcgaaagaa gccgacggcc tggcagacgt
     3121 tggttcgccg ctgccgggtc gggaagtgaa aatcgttaat aatgaagtgt ggctgcgggc
     3181 tgccagtatg gcagaaggtt actggcgtaa cgggcaactg gtttcactgg ttaatgacga
     3241 aggctggtac gctacgcgcg atcgcggtga gatgcataat ggcaagctga ccattgtcgg
     3301 acgtttagac aatctattct tcagtggcgg agagggtatt cagccggaag aagtcgagcg
     3361 tgttattgct gcacatcctg cggttttgca ggtgtttatc gttcccgttg ccgacaagga
     3421 gtttggtcat cggccggtag cggtggtgga gtatgaccag caaaccgttg atcttggcga
     3481 atgggtgaaa gataagctgg ctcgttttca acaaccggtg cgctggctga ctttaccgcc
     3541 ggagctgaaa aacggcggta ttaaaatttc acgtcaggcg ctaaaagagt gggtgcaacg
     3601 tcagcaataa ttaaccctcc gtttcacttc tcatttctgc cattccattg ccgttgataa
     3661 agagtttatc tgttaatctg taaattgatg tgaaaactct tagcaaacag gataatgcaa
     3721 tggaatggct ggtcaaaaaa tcgtgctgca ataaacaaga taacagacat gtacttatgc
     3781 tctgcgatgc tggcggcgcg ataaaaatga tcgccgaagt gaagagtgac ttcgccgtga
     3841 aagtggggga tttactctcg cctttgcaga atgcgcttta ttgtattaat cgtgaaaagc
     3901 tgcatacggt gaaagtcctc tcggcaagca gttatagccc cgatgagtgg gaacggcagt
     3961 gtacagcggc agggaaaact cagtaacgtt gttttgtcgt gggcaggaaa atcagcatca
     4021 acccgctcat aatacaggct actcccagta gtgctttcag cgaaaaagtc ccttcccagc
     4081 cgggaagtac cattgaagca atccatacca gcacataact catactgagc aaggcatagg
     4141 ctttgcttag cgcaagttta tgtagcgttt tataccaaca aaaaacggaa agcagataac
     4201 ccagcagccc gagtaacagt atccgggcat ccaggccaaa agctaacaga gccgcgataa
     4261 aatcccataa gtgcgtcatc ggcggcagat gacttgccgc aaaacccagg cttaattgcg
     4321 caactgaggc aataatgacg ctgaataatc cccatatcag gcccattaca ccgtactccc
     4381 gaggatcaca atgccaccaa taatgaacgc caccccacac cagtgacgcg gcgataccgg
     4441 ttcgtgccac agttttactg cagccagcgt cacccagaca aaattcagac ttaacatcgg
     4501 gtaggcaatg cctaccggta cgttctgcaa gaccagcagc cagagcacca tggcaagacc
     4561 aagacaagcc agcgccagtc ccagccacag cacgatatgt ttgcgccgtt tgtttatcgc
     4621 cacaaagcag gttgcctgtt tctgacacaa ctgcccggca acgctaagca agctggcaaa
     4681 gactaatgtt agccagatca tttgggacga tactgaatca gcaccagacg ctcctgacga
     4741 tcgatggcat ctgcgggcgg aatggcgaga ctgttgatat cttcatcgcg gtcaaccgaa
     4801 agcacgagag taataatccc ctcctgacga tgttgattaa gccagtttgc gaactcatca
     4861 ccgctgacaa atctcccttt cgcatccgga taattaaggc cgtatttcaa ctcacctgtc
     4921 tggcgataca tgatgatgtc atcgcgttgc aggctccatg ccagacctgc ggcaacaccg
     4981 acgctatcag taagaatata gcggcttggc tgaagtgatt cttgtgtcat ctcgacaaaa
     5041 aattgcggat gttttccttc cataactctg tcaggaattg aaaatcctac cagcaacgcc
     5101 agccccagcg ggcaaagcgc ggcaaaaggc caggtctttt cgacgtttgt taaggtgtac
     5161 cagccgaaaa atgcccatag cgaaaaaatc gaccaggcac aaaagacttt atagctctcg
     5221 aaggtttgcc acaccggcgt gttcattggt ccccacgggg agaccacaaa tgtggcaata
     5281 atgccagtga cgccaaaagc gatgttaatc cagccattaa tccgcagcgc cagaggatta
     5341 ttttttgctg ccagcaaagc gtaatgcgcc atcagcattg ccagagatgc aaagcaggaa
     5401 agaatatagg tgggcaattt acctttagcg acggagaaaa acagcagcgg cattatcgtc
     5461 cagctcaaca aatagacggt tgcggaatgc ttgcggtttt tccagcctgt gtacagtgca
     5521 ccggggagta atcccagcca cggcaggcta ccggcaatga tgaccggcac gtagtaccag
     5581 aacggagctc tatgttgggc atcatccagt gcaaagcgtt gaatatgctc aacccagaaa
     5641 aaatagtgcc agaagtcagg ctcccgctgc gctatcgcca gtccccaagg gagaaccgtc
     5701 agtacgcaac tgataaccgc cagccagccg taaataaaga gatctttcca gcgtttttgc
     5761 gtcgctaccc atggcagcac gcttaatacc ggcacggcaa gggcgagaaa acctttggtc
     5821 atcaccccca tgccgcaggt gattcccagc agtaaaaatc ctgcgctttt gcctttccac
     5881 gtctgtgcct gcattgccag ccagaagctg cacattcccg ccaccagcca gaatgcaata
     5941 aacggatcga gcacggcata agtgccgatg gcatagacaa taaacaatga gagataaatt
     6001 actgtggcga gtagagccag acgtttatcg cgccataagc gcagagtaaa ccaggtcacc
     6061 agcgcggcag ttaacagggt cgcaaagata acgcctgccc gcacaccaaa gttattcgcg
     6121 ccaaatagcc attgcccaat gctgttaatc cagtatccgg caatgggttt ttcgaaataa
     6181 cgtagcccta acagatgggg cacaatccag tcgccggatg ccagcatttc tcgactgatt
     6241 tccgcataac gcgtttcatc gggttgccag agcagacgcg tgctgatcgg taacaggtaa
     6301 tagcaggcaa taaacgcgaa gaggccgata aggtaacgta ccgatttcat cagcgactac
     6361 ccgcaatttg ttggcaaccc agccagcctt cacgtccggc aatatttccg cgaacaactt
     6421 gtccgagcgg caacgtctct gataacagtt cgcttaaagg gcaaaatgtc actccttcct
     6481 gagctgcgcg tttgaggaga tccacaaaat tatgctgata agcgcagcct tcaacttctg
     6541 catgaatggt ataaaccggc gtgcctttat ctcgcaggat gcggttgagt aaccaaccgt
     6601 taaaatcttc tgctttcaca tcccgaccaa tcacttcatc ccaggtgggt aaggtcaccg
     6661 gaatttgcgc agtgccaggg tttcctgatt cgagcaatgg acggaacggc atggccccac
     6721 gacaatcgct gttgtagcgc aaatggaacg cttcttttgc ttcgatcacc ttctggtcgg
     6781 cacgccagcc cgcagcggca gaacaggtta ccggttgacc gataatctct tccagagtgc
     6841 gaagaccgcg tgcaatatcg tcgatcattg tttgccgatc ccagttaccg ctacgggctt
     6901 gccaggcatg gtgatcccag gcgtgcaggc cgacttcgtg atgtttagcc gcttcacgaa
     6961 tgatatcggc attggcatga ccaatctctt tacctggcca ggccgtacct gccagtaaaa
     7021 tatcccagcc ataaagcgat gccgcatttg agcgcagcat cttccacaaa aactgtggct
     7081 tcaccagtcg ccagagatgg cggcccatat tgtccgggcc gacgctgaaa aaaatgctgg
     7141 cctgaatatt atgcttactc aagatttcca gcagacgcgg cacgccttca cgggtgccac
     7201 gaaaggtatc gacatcaatg cgtaagccta ctttggtcat gatggtttat ccgtaagatc
     7261 aacggtgcgc aggaagaaat ccagcgtttc gtcgatggtt tcctgcatat caattttcgg
     7321 ctcccagtcc aggcagcggt gggcattgcg gatgctcggt ttacgatgct ctacgtcctg
     7381 atatcctttg ccgtagtagc tgctactttc gacaacgcga aagcccgcaa acggtgggaa
     7441 atgatggcgc agcggatgtt tttcgaagct cgccagcagc atctcgccca gttcctcaat
     7501 gctcgcttcg ttctcaggat tgccaatgtt gataatttca ccgtcgcagc gatttcccgc
     7561 attttcgata atgcggtata acgcctcgat accatcgcga atatcagtaa agcagcgttt
     7621 ttgttttccg ccatcaatca gcttaatcgg tgaaccttct accagattga gaatgagttg
     7681 cgtaatagcg cgggagctgc cgattcgtgc cgcattaagg ttatccagtc gcggtcccat
     7741 ccagttaaac gggcggaaga gggtgaactg taaaccctct ttttcgccat aggcccagat
     7801 cacccgatca agtaattgtt ttgataccga ataaatccag cgtggtttat tcaccgggcc
     7861 gacgattaaa ttagaatggt cctcatcgaa gtatttatcg ctacacatcc cataaacttc
     7921 tgaagttgac gggaagatga ttcgcttacg gtacttcacg cagtagcgga taatgcgcag
     7981 attctcttca aaatcgagtt caaatacgcg cagcgggttg cgggtatatt caatcggcgt
     8041 ggctatcgcc accagcggca agacgacatc acattttttg acgtgatact caatccattc
     8101 ggaatgaata ctgatatcgc cttcaacaaa gtgaaaatgc ggatgattca gaaaacggct
     8161 tatcgcatca ctgccaatat ccagaccgta aacttcataa tgatcttcgc gcagcaggcg
     8221 ttctgtcaga tggttgccaa taaagccatt caccccgagg atgagtaccc gggtacggcg
     8281 tcgggcggtg caggcaggct ggctattcaa gcgtgaacct tgcaccaggc ccagcgtctg
     8341 cgctaattgc gagccctgca tagtaatgcc gtcgcccgcc tgtccggtga cgatttccag
     8401 cgcgccatcg ccacaggcaa tcagcagtgg cgcaacagaa atcacgctcc ccggctgtgc
     8461 tttgctggca tgaggatgaa cacgcgacga ccagacggtg aatttctgat tgccaacata
     8521 gctgaaggca cccggccacg gatcggcaac ggcacgtacc atgttgtgca gtacggatgc
     8581 cggtttatgc cattcaagga aactgtcatc cggcgttctg cgaccaaaac aggtggcttc
     8641 gttttcgcgc tgggcgattt ccagaatatt accgtgttta atggcgggta atgtctgttc
     8701 cagtagctgg cgcgcggcat ggcacaattt atgatgcagc gtaatagcga tatcgtctgg
     8761 cgcaatggca atgcgcagtt gcgccacaat ggccccggca tcggcacgtt tcaccattcg
     8821 gtgcaatgta acgccagttt ccgtttcacc gttgaccagc acccagttca gcggcgcgcg
     8881 accacgatat tttggtaaca gcgaaccatg cagattaaat gcacctgcgg gagcgagctg
     8941 caaaatttcg tcgcaaataa gatggcgata ataaaaagag aaaatcacat ctggcgacag
     9001 ttgggcaatg cgttccaccc acagcggatg attaacgtca tccggcgcat aaaccggaat
     9061 gcctctttcc gctgccagac gagccaccga accataaaag gctttttcac cgggattatc
     9121 ggtatgggta aaaatggcgc taatttcgta accggcagcc agcagagctt caataccgag
     9181 gcatcccata tcgtggtagg caaaaacgac ggttttcatt attcattttc cttgctggat
     9241 ggacggataa cttgctgaac aaaatagcgg gggcgggcgc ggacatcggt gtagatcctg
     9301 ccgatatatt caccgagtaa tcccatgccg ataaactgag cgccaataaa agtaaacagc
     9361 acggcaaata gcataaagac gccttctgcc gcccattgtg gtccgaaggt caggcgtaaa
     9421 atcaccagca gcaccgcaat gctaaaacct ccaatcgcaa taatgctgcc gagcagactc
     9481 agcatacgta gcggcgttgt ggtaaggcag gtcaccaggt cgtacatcaa attaatcagg
     9541 cgcataaaac tgtatttgga ttcaccaaac tcacgctcgg aatgatgtac tggaatttca
     9601 atggcacggc gggcgaagat attcgccaga atcgggataa aggtgctacg ttcatggcag
     9661 tgcaacatcg catcgacaat atgacggcga taggcgcgca gcatacaacc gtagtcaccc
     9721 atcgctttgc cggtggtgcg ctgaataagc cggttaatca tcttcgaagc tgttttacga
     9781 aaccagctgt cctggcggtt ctggcgtaca gtccctacca cgtcgtaacc ttcatcggct
     9841 tttgccacca ggcgggggat ttcttctggc ggattctgga gatcggcatc aagggtaata
     9901 attaagtcgc cggtaacgtg actgaatccc gccataatcg ctgaatgttg cccgtaattg
     9961 cggttaagca aaatagacac aatatggctg ttctccgctt gtgaggcttc gaccagcata
    10021 tgcgcggaat tatcgctact gccgtcatca atcagcagga tctcatactc tttccccaac
    10081 gattcacagg ctgtggtggt gcgcctgatt aattccggta agctttcctg ctcgttataa
    10141 acgggaataa ccaccgagac tttcttaaca gggtggattt caaacatggc ttattgtcct
    10201 gcgagttgct gaagggctgt gatgacacgg tcggcatcgg cggtagtcat atccgggaac
    10261 agcggcaaag aacagatgcg ttcgctattc cattcggtat tcggtaacga cagcgtggga
    10321 aaacgctcgc gataatattt ttgtgtgtga gcggcgcgga aatgtaaccc ggtaccaatg
    10381 cctctttctt ttaacgcttc catcaacgca tcgcgactga taccacaacg ttgttcatcg
    10441 acacgaataa taaacagatg ccaggcgtga acgtgcggcc aggcgggaag gcttaatggc
    10501 tgaaagggga gagctgccag tgcttgctga tattgctggg caatttcgcg ccgacgggtg
    10561 ttgaggtgct ctaattttgc taactgtgtc agggcaattg cggcgttaat atcggtcaga
    10621 ttgtacttat agcccggtgt taagacttca gcctgcggtg cacggcccca ggtttgtctg
    10681 tcataggcat cgacacccag accgtgaaat ttcagcatcc gtagctggcg ggcaaggttt
    10741 tcattatcag ttacaatcag gccaccttcc gcacaggtaa tatttttaat ggcatgaaat
    10801 gaaaaaatag tggtaccttt tgcgccaata tgtcgccctt tgtaatacgt accgacggca
    10861 tgggcagcat cttcgataac tgcgatgccg taacgttcgc caatggcgcg aatggcgtca
    10921 atatctgctg gcgcaccggc ataatgcacc ggaatgatgg ctttagtgcg tggcgttatg
    10981 gctgactcga tcgcttcagg cgtgaccatc ggcgtatcgc ggtcgacatc caccattacc
    11041 ggcgttgcac ccaacaagga aatcatattg agggttgaaa cccaggtcag ggaaggcgta
    11101 atcacttcat cgcccttgcc aatttccaac gccattagcg tgatatgcat tccggcggtg
    11161 gctgaactga ccgcgatggc atgctgattt cccgtcaact ggcaaaaggc ttgctcaagc
    11221 gcctgattct tcggaccggt tgtgatccaa ccggattcga gaacctcttt aactgcagcg
    11281 agttcctcca cgcccattgc tggtcgcgaa aaaggcaaaa attctgacat tgcttttcct
    11341 tccgccattg aatacctgtc cacttatat
//
LOCUS       DAZKHF020000144        11314 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2201S31241
            SAMN43267800-rid25106413.denovo.145, whole genome shotgun sequence.
ACCESSION   DAZKHF020000144 DAZKHF020000000
VERSION     DAZKHF020000144.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267800
            Sequence Read Archive: SRR30307263
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 11314)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11314)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 11314)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350581.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350581.2
            Genome Coverage       :: 151x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:21:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,652
            CDSs (total)                      :: 5,557
            Genes (coding)                    :: 5,320
            CDSs (with protein)               :: 5,320
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 237
            CDSs (without protein)            :: 237
            Pseudo Genes (ambiguous residues) :: 0 of 237
            Pseudo Genes (frameshifted)       :: 102 of 237
            Pseudo Genes (incomplete)         :: 130 of 237
            Pseudo Genes (internal stop)      :: 52 of 237
            Pseudo Genes (multiple problems)  :: 41 of 237
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11314
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267800-rid25106413.denovo.145"
                     /serotype="O 26:H11"
                     /isolate="2201S31241"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-01"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            476..2404
                     /gene="thrS"
                     /locus_tag="ACHOSC_RS15420"
                     /old_locus_tag="pgaptmp_003084"
     CDS             476..2404
                     /gene="thrS"
                     /locus_tag="ACHOSC_RS15420"
                     /old_locus_tag="pgaptmp_003084"
                     /EC_number="6.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006894721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004829 - threonine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006435 - threonyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine--tRNA ligase"
                     /protein_id="HGU4548981.1"
                     /translation="MPVITLPDGSQRHYDHAVSPMDVALDIGPGLAKACIAGRVNGEL
                     VDACDLIENDAQLSIITAKDEDGLEIIRHSCAHLLGHAIKQLWPHTKMAIGPVIDNGF
                     YYDVDLDRTLTQEDVEALEKRMHELAEKNYDVIKKKVSWHEARETFANRGESYKVSIL
                     DENIAHDDKPGLYFHEEYVDMCRGPHVPNMRFCHHFKLMKTAGAYWRGDSNNKMLQRI
                     YGTAWADKKALNAYLQRLEEAAKRDHRKIGKQLDLYHMQEEAPGMVFWHNDGWTIFRE
                     LEVFVRSKLKEYQYQEVKGPFMMDRVLWEKTGHWDNYKDAMFTTSSENREYCIKPMNC
                     PGHVQIFNQGLKSYRDLPLRMAEFGSCHRNEPSGSLHGLMRVRGFTQDDAHIFCTEEQ
                     IRDEVNGCIRLVYDMYSTFGFEKIVVKLSTRPEKRIGSDEMWDRAEADLAVALEENNI
                     PFEYQLGEGAFYGPKIEFTLYDCLDRAWQCGTVQLDFSLPSRLSASYVGEDNERKVPV
                     MIHRAILGSMERFIGILTEEFAGFFPTWLAPVQVVIMNITDSQSEYVNELTQKLSNAG
                     IRVKADLRNEKIGFKIREHTLRRVPYMLVCGDKEVESGKVAVRTRRGKDLGSMDVNEV
                     IEKLQQEIRSRSLKQLEE"
     gene            2408..2950
                     /gene="infC"
                     /locus_tag="ACHOSC_RS15425"
                     /old_locus_tag="pgaptmp_003085"
     CDS             2408..2950
                     /gene="infC"
                     /locus_tag="ACHOSC_RS15425"
                     /old_locus_tag="pgaptmp_003085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-3"
                     /protein_id="HGU4548982.1"
                     /translation="MKGGKRVQTARPNRINGEIRAQEVRLTGLEGEQLGIVSLREALE
                     KAEEAGVDLVEISPNAEPPVCRIMDYGKFLYEKSKSSKEQKKKQKVIQVKEIKFRPGT
                     DEGDYQVKLRSLIRFLEEGDKAKITLRFRGREMAHQQIGMEVLNRVKDDLQELAVVES
                     FPTKIEGRQMIMVLAPKKKQ"
     gene            3047..3244
                     /gene="rpmI"
                     /locus_tag="ACHOSC_RS15430"
                     /old_locus_tag="pgaptmp_003086"
     CDS             3047..3244
                     /gene="rpmI"
                     /locus_tag="ACHOSC_RS15430"
                     /old_locus_tag="pgaptmp_003086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006708231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L35"
                     /protein_id="HGU4548983.1"
                     /translation="MPKIKTVRGAAKRFKKTGKGGFKHKHANLRHILTKKATKRKRHL
                     RPKAMVSKGDLGLVIACLPYA"
     gene            3297..3653
                     /gene="rplT"
                     /locus_tag="ACHOSC_RS15435"
                     /old_locus_tag="pgaptmp_003087"
     CDS             3297..3653
                     /gene="rplT"
                     /locus_tag="ACHOSC_RS15435"
                     /old_locus_tag="pgaptmp_003087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L20"
                     /protein_id="HGU4548984.1"
                     /translation="MARVKRGVIARARHKKILKQAKGYYGARSRVYRVAFQAVIKAGQ
                     YAYRDRRQRKRQFRQLWIARINAAARQNGISYSKFINGLKKASVEIDRKILADIAVFD
                     KVAFTALVEKAKAALA"
     misc_feature    3770..3894
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01859"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Phe leader region; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /db_xref="RFAM:RF01859"
     gene            3776..3820
                     /gene="pheM"
                     /locus_tag="ACHOSC_RS15440"
                     /old_locus_tag="pgaptmp_003088"
     CDS             3776..3820
                     /gene="pheM"
                     /locus_tag="ACHOSC_RS15440"
                     /old_locus_tag="pgaptmp_003088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0031556 - transcriptional attenuation by
                     ribosome [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pheST operon leader peptide PheM"
                     /protein_id="HGU4548985.1"
                     /translation="MNAAIFRFFFYFST"
     gene            4104..5087
                     /gene="pheS"
                     /locus_tag="ACHOSC_RS15445"
                     /old_locus_tag="pgaptmp_003089"
     CDS             4104..5087
                     /gene="pheS"
                     /locus_tag="ACHOSC_RS15445"
                     /old_locus_tag="pgaptmp_003089"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004851795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004826 - phenylalanine-tRNA ligase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006432 - phenylalanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit alpha"
                     /protein_id="HGU4548986.1"
                     /translation="MSHLAELVASAKAAISQASDVAALDNVRVEYLGKKGHLTLQMTT
                     LRELPPEERPAAGAVINEAKEQVQQALNARKAELESAALNARLAAETIDVSLPGRRIE
                     NGGLHPVTRTIDRIESFFGELGFTVATGPEIEDDYHNFDALNIPGHHPARADHDTFWF
                     DTTRLLRTQTSGVQIRTMKAQQPPIRIIAPGRVYRNDYDQTHTPMFHQMEGLIVDTNI
                     SFTNLKGTLHDFLRNFFEEDLQIRFRPSYFPFTEPSAEVDVMGKNGKWLEVLGCGMVH
                     PNVLRNVGIDPEVYSGFAFGMGMERLTMLRYGVTDLRSFFENDLRFLKQFK"
     gene            5102..7489
                     /gene="pheT"
                     /locus_tag="ACHOSC_RS15450"
                     /old_locus_tag="pgaptmp_003090"
     CDS             5102..7489
                     /gene="pheT"
                     /locus_tag="ACHOSC_RS15450"
                     /old_locus_tag="pgaptmp_003090"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004826 - phenylalanine-tRNA ligase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006432 - phenylalanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit beta"
                     /protein_id="HGU4548987.1"
                     /translation="MKFSELWLREWVNPAIDSDALANQITMAGLEVDGVEPVAGSFHG
                     VVVGEVVECAQHPNADKLRVTKVNVGGDRLLDIVCGAPNCRQGLRVAVATIGAVLPGD
                     FKIKAAKLRGEPSEGMLCSFSELGISDDHSGIIELPADAPIGTDIREYLKLDDNTIEI
                     SVTPNRADCLGIIGVARDVAVLNQLPLVEPEIVPVGATIDDTLPITVEAPEACPRYLG
                     RVVKGINVKAPTPLWMKEKLRRCGIRSIDAVVDVTNYVLLELGQPMHAFDKDRIEGGI
                     VVRMAKEGETLVLLDGTEAKLNADTLVIADHNKALAMGGIFGGEHSGVNDETQNVLLE
                     CAFFSPLSITGRARRHGLHTDASHRYERGVDPALQHKVMERATRLLIDICGGEAGPVI
                     DITNEATLPKRATITLRRSKLDRLIGHHIADEQVTDILRRLGCEVTEGKDEWQAVAPS
                     WRFDMEIEEDLVEEVARVYGYNNIPDEPVQASLIMGTHREADLSLKRVKTLLNDKGYQ
                     EVITYSFVDPKVQQMIHPGVEALLLPSPISVEMSAMRLSLWTGLLATVVYNQNRQQNR
                     VRIFESGLRFVPDTQAPLGIRQDLMLAGVICGNRYEEHWNLAKETVDFYDLKGDLESV
                     LDLTGKLNEVEFRAEANPALHPGQSAAIYLKGERIGFVGVVHPELERKLDLNGRTLVF
                     ELEWNKLADRVVPQAREISRFPANRRDIAVVVAENVPAADILSECKKVGVNQVVGVNL
                     FDVYRGKGVAEGYKSLAISLILQDTSRTLEEEEIAATVAKCVEALKERFQASLRD"
     gene            7494..7793
                     /gene="ihfA"
                     /locus_tag="ACHOSC_RS15455"
                     /old_locus_tag="pgaptmp_003091"
     CDS             7494..7793
                     /gene="ihfA"
                     /locus_tag="ACHOSC_RS15455"
                     /old_locus_tag="pgaptmp_003091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="integration host factor subunit alpha"
                     /protein_id="HGU4548988.1"
                     /translation="MALTKAEMSEYLFDKLGLSKRDAKELVELFFEEIRRALENGEQV
                     KLSGFGNFDLRDKNQRPGRNPKTGEDIPITARRVVTFRPGQKLKSRVENASPKDE"
     gene            7894..8874
                     /gene="btuC"
                     /locus_tag="ACHOSC_RS15460"
                     /old_locus_tag="pgaptmp_003092"
     CDS             7894..8874
                     /gene="btuC"
                     /locus_tag="ACHOSC_RS15460"
                     /old_locus_tag="pgaptmp_003092"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="vitamin B12 ABC transporter permease BtuC"
                     /protein_id="HGU4548989.1"
                     /translation="MLTLARQQQRQNIRWLLCLSVLMLLALLLSLCAGEQWISPGDWF
                     TPRGELFVWQIRLPRTLAVLLVGAALAISGAVMQALFENPLAEPGLLGVSNGAGVGLI
                     AAVLLGQGQLPNWALGLCAIAGALIITLILLRFARRHLSTSRLLLAGVALGIICSALM
                     TWAIYFSTSVDLRQLMYWMMGGFGGVDWRQSWLMLALIPVLLWICCQSRPMNMLALGE
                     ISARQLGLPLWFWRNVLVAATGWMVGVSVALAGAIGFIGLVIPHILRLCGLTDHRVLL
                     PGCALAGASALLLADIVARLALAAAELPIGVVTATLGAPVFIWLLLKARR"
     gene            8937..9488
                     /gene="btuE"
                     /locus_tag="ACHOSC_RS15465"
                     /old_locus_tag="pgaptmp_003093"
     CDS             8937..9488
                     /gene="btuE"
                     /locus_tag="ACHOSC_RS15465"
                     /old_locus_tag="pgaptmp_003093"
                     /EC_number="1.11.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional thioredoxin/glutathione peroxidase"
                     /protein_id="HGU4548990.1"
                     /translation="MQDSILTTVVKDIDGEVTTLEKYAGNVLLIVNVASKCGLTPQYE
                     QLENIQKAWADRGFVVLGFPCNQFLEQEPGSDEEIKTYCTTTWGVTFPMFSKIEVNGE
                     GRHPLYQKLIAAAPTAVAPEESGFYARMVSKGRAPLYPDDILWNFEKFLVGRDGLVIQ
                     RFSPDMTPEDPIVMESIKLALAK"
     gene            9488..10237
                     /gene="btuD"
                     /locus_tag="ACHOSC_RS15470"
                     /old_locus_tag="pgaptmp_003094"
     CDS             9488..10237
                     /gene="btuD"
                     /locus_tag="ACHOSC_RS15470"
                     /old_locus_tag="pgaptmp_003094"
                     /EC_number="7.6.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0015889 - cobalamin transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="vitamin B12 ABC transporter ATP-binding protein
                     BtuD"
                     /protein_id="HGU4548991.1"
                     /translation="MSIVMQLQDVAETTRLGPLSGEVRAGEILHLVGPNGAGKSTLLA
                     RMAGMTSGKGSIQFAGQPLEAWSATKLALHRAYLSQQQTPPFAMPVWHYLTLHQHDKT
                     RTELLNDVAGALALDDKLGRSTNQLSGGEWQRVRLAAVVLQITPQANPAGQLLLLDEP
                     MNSLDVAQQSALDKILSALCQQGLAIVMSSHDLNHTLRHAHRAWLLKGGKMLASGRRE
                     EVLTPPNLAQAYGMNFRRLDIEGHRMLISTI"
     gene            10315..10779
                     /gene="nlpC"
                     /locus_tag="ACHOSC_RS15475"
                     /old_locus_tag="pgaptmp_003095"
     CDS             10315..10779
                     /gene="nlpC"
                     /locus_tag="ACHOSC_RS15475"
                     /old_locus_tag="pgaptmp_003095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NlpC/P60 family protein"
                     /protein_id="HGU4548992.1"
                     /translation="MRFCLILITALFLAGCSHHKAPPPNARLSDSITVIAGLNDQLQS
                     WHGTPYRYGGMTRRGVDCSGFVVVTMRDRFDLQLPRETKEQASIGTQIDKDELLPGDL
                     VFFKTGSGQNGLHVGIYDTNNQFIHASTSKGVMRSSLDNVYWQKNFWQARRI"
     gene            11026..>11314
                     /gene="ydiV"
                     /locus_tag="ACHOSC_RS15480"
                     /old_locus_tag="pgaptmp_003096"
     CDS             11026..>11314
                     /gene="ydiV"
                     /locus_tag="ACHOSC_RS15480"
                     /old_locus_tag="pgaptmp_003096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anti-FlhDC factor"
                     /protein_id="HGU4548993.1"
                     /translation="MKIFLENLYHSDCYFLPIRDNQQDLVGVELITHFSSEDGTVRIP
                     TSRVIAQLTEEQHWQLFSEQLELLKSCQHFFIQHKLFAWLNLTPQVATLLLE"
BASE COUNT         2858 a         2645 c         3023 g         2788 t
ORIGIN      
        1 ttattatcgc cagaacgaaa tgtgcacgaa aaattgttat aagaattgtg tcacttttat
       61 cattgtgata tgaccacttc gtcattaatc tcatgagcgt ttaaaatact accgccaaat
      121 cacgcaacga actttgctgg attcagttta tgctgctgta aatccgctcg cagtaaacct
      181 ttcagacgca ccgtgatgtt atcagtggtt ctttaagcgt tttgctggtg tactcactac
      241 aaacgaattg cgaatcgaat caatgtgaaa cggaaaggta caatctccct ttttttagtg
      301 ttaacatcgc tcaaccgggt ggaaggtgag aggttaatgt ctttcatttg tgtacctgat
      361 tgcgaaccaa tttagcattt gttggctaga tggtttcgca atgaactgtt aataaacaaa
      421 tttttctttg tatgtgatct ttcgtgtggg tcaccactgc aaataaggat ataaaatgcc
      481 tgttataact cttcctgatg gcagccaacg ccattacgac cacgctgtaa gccccatgga
      541 tgttgcgctg gacattggtc caggtctggc gaaagcctgt atcgcagggc gcgttaatgg
      601 cgaactggtt gatgcttgcg atctgattga aaacgacgca caactgtcca tcattaccgc
      661 caaagacgaa gacggtctgg agatcattcg tcactcctgt gcgcacctgt tagggcacgc
      721 gattaaacaa ctttggccgc ataccaaaat ggcaatcggc ccggttattg acaacggttt
      781 ttattacgac gttgatcttg accgcacgtt aacccaggaa gatgtcgaag cactcgagaa
      841 gcggatgcat gagcttgctg agaaaaacta cgacgtcatt aagaagaaag tcagctggca
      901 cgaagcgcgt gaaactttcg ccaaccgtgg ggagagctac aaagtctcca ttcttgacga
      961 aaacatcgcc catgatgaca agccaggtct gtacttccat gaagaatatg tcgatatgtg
     1021 ccgcggtccg cacgtaccga acatgcgttt ctgccatcat ttcaaactaa tgaaaacggc
     1081 aggggcttac tggcgtggcg acagcaacaa caaaatgttg caacgtattt acggtacggc
     1141 gtgggcagac aaaaaagcgc ttaacgctta cctgcagcgc ctggaagaag ccgcgaaacg
     1201 cgaccaccgt aaaatcggta aacagctcga cctgtaccat atgcaggaag aagcgccggg
     1261 tatggtattc tggcacaacg acggctggac catcttccgt gaactagaag tgtttgttcg
     1321 ttctaaactg aaagagtacc agtatcagga agttaaaggt ccgttcatga tggaccgtgt
     1381 cctgtgggaa aaaaccggtc actgggacaa ctacaaagat gcaatgttca ccacgtcttc
     1441 tgagaaccgt gaatactgca ttaagccgat gaactgcccg ggtcacgtac aaattttcaa
     1501 ccaggggctg aagtcttatc gcgatctgcc gctgcgtatg gccgagtttg gtagctgcca
     1561 ccgtaatgag ccgtcaggtt cgctgcatgg cctgatgcgc gtgcgtggat ttacccagga
     1621 tgacgcgcat atcttctgta ctgaagaaca aattcgcgat gaagttaacg gatgtatccg
     1681 tttagtctat gatatgtaca gcacttttgg cttcgagaag atcgtcgtca aactctccac
     1741 tcgtcctgaa aaacgtattg gcagtgatga aatgtgggat cgtgctgagg cggacctggc
     1801 ggttgcgctg gaagaaaaca acatcccgtt tgaatatcaa ctgggtgaag gcgctttcta
     1861 cggtccgaaa attgaattta ccctgtatga ctgcctcgat cgtgcatggc agtgcggtac
     1921 agtacagctg gacttctctt tgccttctcg tctgagcgct tcttatgtag gcgaagacaa
     1981 tgaacgtaaa gtaccggtaa tgattcaccg cgcaattctg ggatcgatgg aacgtttcat
     2041 cggtatcctg accgaagaat tcgctggttt cttcccgacc tggcttgcgc cagttcaggt
     2101 tgttatcatg aatattaccg attcacagtc tgaatacgtt aacgaattga cgcaaaaact
     2161 atcaaatgcg ggcattcgtg ttaaagcaga cttgagaaat gagaagattg gctttaaaat
     2221 ccgcgagcac actttgcgtc gcgtcccata tatgctggtc tgtggtgata aagaggtgga
     2281 atcaggcaaa gttgccgttc gcacccgccg tggtaaagac ctgggaagca tggacgtaaa
     2341 tgaagtgatc gagaagctgc aacaagagat tcgcagccgc agtcttaaac aattggagga
     2401 ataaggtatt aaaggcggaa aacgagttca aacggcgcgc cctaaccgta tcaatggcga
     2461 aattcgcgcc caggaagttc gcttaacagg tctggaaggc gagcagcttg gtattgtgag
     2521 tctgagagaa gctctggaga aagcagaaga agccggagta gacttagtcg agatcagccc
     2581 taacgccgag ccgccggttt gtcgtataat ggattacggc aaattcctct atgaaaagag
     2641 caagtcttct aaggaacaga agaaaaagca aaaagttatc caggttaagg aaattaaatt
     2701 ccgtcctggt acagatgaag gcgactatca ggtaaaactc cgcagcctga ttcgctttct
     2761 cgaagagggt gataaagcca aaatcacgct gcgtttccgc ggtcgtgaga tggcgcacca
     2821 gcaaatcggt atggaagtgc ttaatcgcgt gaaagacgat ttgcaagaac tggcagtggt
     2881 cgaatccttc ccaacgaaga tcgaaggccg tcagatgatc atggtgctcg ctcctaagaa
     2941 gaaacagtaa ggccttcaag tagcactgtc tgtggagcct tcgggcttca caggctttgt
     3001 tcgcctttgt ttcgtttatt aacaatgcga agtggaagtt attaaaatgc caaaaattaa
     3061 gaccgtacgc ggtgctgcta agcgcttcaa aaaaaccggt aaaggtggtt ttaagcacaa
     3121 gcacgctaac ctgcgtcaca ttctgaccaa aaaagcgacc aaacgtaaac gtcacctgcg
     3181 tccgaaagcc atggtttcca aaggcgatct gggcctggta atcgcgtgcc tgccgtacgc
     3241 ataagccgtt aacgttttta actttttaat tagaatatag atacaggaga gcacatatgg
     3301 ctcgcgtaaa acgtggtgtt attgcacgcg cacgtcacaa gaaaattttg aaacaagcta
     3361 aaggctacta cggtgcgcgt tctcgcgtat accgcgttgc cttccaggct gttatcaaag
     3421 ctggtcagta tgcttaccgt gaccgtcgtc aacgtaagcg tcagttccgt caactgtgga
     3481 ttgcgcgtat caacgcagca gcacgtcaga acggtatttc ttacagcaaa ttcatcaatg
     3541 gcctgaaaaa agcctctgtt gaaatcgacc gtaagatcct ggctgatatc gcagtattcg
     3601 acaaagtagc gttcaccgct ctggttgaaa aagcgaaagc agctctggca taagccagtt
     3661 gaaagaggga gctagtctcc ctcttttcgt ttcaacgcca tcaaaacatt gacttttatc
     3721 gccgtagcct tttcaataaa ggtcttttga agagtaccaa aaggtaacgc aagcaatgaa
     3781 tgctgctatt ttccgcttct ttttttactt tagcacctga atccaggagg ctagcgcgtg
     3841 agaagagaaa cggaaaacag cgcctgaaag cctcccagtg gaggcttttt ttgtatgcgc
     3901 gttttgaaaa ataaaaagcc tgtctcatca ggtgtaattg tcgcagccag tctggacacg
     3961 gccagcgcgg agaaaccgga gcgtatttaa gtacgtgaga atttcgagca ctgcccggga
     4021 ccaaaatggc aagtaaaata gcctgatggg ataggctcta agtccaacga accagtgtca
     4081 ccactgacac aatgaggaaa accatgtcac atctcgcaga actggttgcc agtgcgaagg
     4141 cggccattag ccaggcgtca gatgttgccg cgttagataa tgtgcgcgtc gaatatttgg
     4201 gtaaaaaagg gcacttaacc cttcagatga cgaccctgcg tgagctgccg ccagaagagc
     4261 gtccggcagc tggtgcggtt atcaacgaag cgaaagagca ggttcagcag gcgctgaatg
     4321 cgcgtaaagc ggaactggaa agcgctgcac tgaatgcgcg tctggcggcg gaaacgattg
     4381 atgtctctct gccaggtcgt cgcattgaaa acggcggtct gcatccggtt acccgtacta
     4441 tcgaccgtat cgaaagtttc ttcggtgagc ttggctttac cgtggcaacc gggccggaaa
     4501 tcgaagacga ttatcataac ttcgatgctc tgaacattcc tggtcaccac ccggcgcgcg
     4561 ctgaccacga cactttctgg tttgacacta cccgcctgct gcgtacccag acctctggcg
     4621 tacagatccg caccatgaaa gcccagcagc caccgattcg tatcattgcg cctggccgcg
     4681 tttatcgtaa cgactacgac cagactcaca cgccgatgtt ccatcagatg gaaggtctga
     4741 ttgttgatac caacatcagc tttaccaacc tgaaaggcac tctgcacgat ttcctgcgta
     4801 acttctttga ggaagatttg cagattcgct tccgtccttc ctacttcccg ttcaccgagc
     4861 cttctgcaga agttgacgtc atgggtaaaa acggtaaatg gctggaagtg ctgggttgcg
     4921 ggatggtgca tccgaacgtg ctgcgtaacg ttggcatcga cccggaagtt tactctggtt
     4981 tcgccttcgg gatggggatg gagcgtctga ctatgttgcg ttacggcgtc accgacctgc
     5041 gttcattctt cgaaaatgat ctgcgtttcc tcaaacagtt taaataaggc aggaatagat
     5101 tatgaaattc agtgaactgt ggttacgcga atgggtgaac ccggcgattg atagcgatgc
     5161 gctggcgaat caaatcacta tggcgggcct ggaagttgac ggtgtagaac cggttgccgg
     5221 tagcttccac ggcgtggtcg ttggtgaagt ggttgagtgt gcgcagcatc cgaacgctga
     5281 caaactgcgt gtgacaaaag tgaatgttgg cggcgatcgc ctgctggaca tcgtctgcgg
     5341 tgcgccaaac tgccgtcagg gcctgcgtgt ggcggtagct accattggtg ctgttctgcc
     5401 gggtgatttc aaaattaaag cggcgaaact gcgtggtgaa ccgtctgaag ggatgctgtg
     5461 ctccttttct gagctgggaa tttctgacga tcatagcggc attatcgagc tgcctgcgga
     5521 tgcgccgatt ggcaccgaca tccgtgaata cctgaaactt gatgacaaca ccatcgaaat
     5581 cagcgtgacg ccaaaccgtg ccgactgttt aggtatcatt ggtgttgcgc gtgacgttgc
     5641 cgtgctgaat cagctgccgc tggttgaacc ggaaatcgtt ccggttggtg cgaccatcga
     5701 cgacacgctg ccgattacag tcgaagcgcc ggaagcctgc ccgcgttatc ttggccgtgt
     5761 ggtaaaaggc attaacgtta aagcgccaac tccgctgtgg atgaaagaaa aactgcgtcg
     5821 ttgcgggatc cgttctatcg atgcagttgt tgacgtcacc aactatgtac tgctcgaact
     5881 gggccagccg atgcacgctt tcgataaaga tcgcattgaa ggcggcattg tggtgcggat
     5941 ggcgaaagag ggcgaaacgt tggtactgct tgatggcact gaagcgaagc tgaatgctga
     6001 cactctggtc atcgccgacc acaacaaggc gctggcgatg ggcggcatct ttggtggcga
     6061 acactctggc gtgaatgacg aaacgcaaaa cgtcttgctg gaatgcgctt tcttcagccc
     6121 gctgtctatc accggtcgtg ctcgtcgtca tggcctgcat actgatgcgt ctcaccgtta
     6181 tgagcgtggc gttgatccgg cactgcagca caaagtgatg gaacgtgcga cccgtctgct
     6241 gatcgacatc tgcggtggtg aggctggtcc ggtaattgat atcaccaacg aagcaacgct
     6301 gccgaagcgt gcaaccatca ctttacgtcg tagcaaactg gatcgcctga tcggccatca
     6361 tattgcggat gagcaggtaa ctgacattct gcgtcgtctc ggctgcgaag tgaccgaagg
     6421 caaagacgag tggcaggcag ttgcgccgag ctggcgtttc gatatggaga ttgaagaaga
     6481 tctggtcgaa gaagtcgcgc gtgtttacgg ctacaacaac atcccggatg agccggtaca
     6541 ggcaagcctg attatgggta ctcaccgtga agctgacctg tcgctcaagc gcgtgaaaac
     6601 gctgctcaac gacaaaggct atcaggaagt gatcacctac agcttcgttg atccgaaagt
     6661 gcagcagatg atccatccag gcgttgaagc cttactgctg ccaagcccga tctctgttga
     6721 aatgtcagca atgcgtcttt ctctgtggac cggcctgctg gcaaccgtgg tgtacaacca
     6781 gaaccgtcag cagaaccgtg tgcgcatttt cgaaagcggt ctgcgtttcg taccagatac
     6841 tcaggcaccg ttgggcattc gtcaggatct gatgttagcc ggtgtgattt gcggtaaccg
     6901 ttacgaagag cactggaacc tggcaaaaga gaccgttgat ttctatgatt tgaaaggcga
     6961 tcttgaatcc gttctcgacc tgaccggtaa actgaatgag gttgagttcc gtgcagaagc
     7021 gaatccggca ctgcatccgg ggcaatccgc agcgatttat ctgaaaggtg aacgtattgg
     7081 ttttgttggg gttgttcatc ctgaactgga acgtaaactg gatcttaacg gtcgcactct
     7141 ggtgttcgaa ctggagtgga acaagctcgc agaccgcgtg gtgcctcagg cgcgcgagat
     7201 ttctcgcttc ccggcgaacc gtcgtgacat cgcggtggtg gtcgcagaaa acgttcccgc
     7261 agcggatatt ttatccgaat gtaagaaagt tggcgtaaat caggtagttg gcgtaaactt
     7321 atttgacgtg taccgcggta agggtgttgc ggaggggtat aagagcctcg ccataagcct
     7381 gatcctgcaa gataccagcc gtacactcga agaagaggag attgccgcta ccgtcgccaa
     7441 atgtgtagag gcattaaaag agcgattcca ggcatcattg agggattgaa cctatggcgc
     7501 ttacaaaagc tgaaatgtca gaatatctgt ttgataagct tgggcttagc aagcgggatg
     7561 ccaaagaact ggttgaactg tttttcgaag agatccgtcg cgctctggaa aacggcgaac
     7621 aggtgaaact ctctggtttt ggtaacttcg atctgcgtga taagaatcaa cgcccgggac
     7681 gtaacccgaa aacgggcgag gatattccca ttacagcacg gcgcgtggtg accttcagac
     7741 ccgggcagaa gttaaaaagc cgggtcgaaa acgcttcgcc caaagacgag taatctgatc
     7801 taactaaaaa ggccgctctg cggccttttt tcttttcact gtcgaagagt caccgtaaaa
     7861 tcaacgccat gacacttcag cagaacggat accatgctga cacttgcccg ccaacaacag
     7921 cgacaaaata ttcgctggtt attatgcctg tcagttttga tgctgctggc gcttctctta
     7981 agcctttgcg ccggtgaaca atggatctcg ccaggtgact ggtttactcc tcgtggcgaa
     8041 ctgttcgtct ggcaaattcg cctgccacgt acgctggctg tattgctggt tggtgcggcg
     8101 ctggctatat ccggcgctgt aatgcaggcg ttgtttgaaa atcctctggc agaacctgga
     8161 ctacttggcg tctctaacgg cgcaggcgtg gggcttatcg ccgcggtatt gcttgggcaa
     8221 gggcaactcc ccaactgggc gctagggctg tgtgcgattg ctggcgcgct tatcatcact
     8281 ttaatactct tacgtttcgc ccgtcgtcat ctttcgacca gtcggttatt gctggctggc
     8341 gttgcattag ggattatctg tagcgcacta atgacgtggg ctatctactt ttccacctct
     8401 gttgatttac gtcagctgat gtactggatg atgggcggtt ttggcggcgt agactggcgg
     8461 caaagctggc tgatgctggc attgatcccc gtgttgttgt ggatctgttg tcagtccagg
     8521 ccgatgaata tgttagcact tggcgagatc tcggcgcggc aactgggttt acccctgtgg
     8581 ttctggcgca atgtgctggt ggccgcgacc ggctggatgg ttggcgtcag tgtggcgctg
     8641 gcgggtgcta tcggctttat tggtctggtg atcccacata ttcttcggtt gtgtggttta
     8701 accgatcatc gcgtattact tcccggctgc gcgctggcag gggcgagcgc attgctgctg
     8761 gccgatattg tagcgcgcct ggcattagct gccgcagagc tgcctattgg cgtggtcacc
     8821 gcaacgttgg gtgcgccggt gtttatctgg ttattgttaa aagcaagacg ttagccgcaa
     8881 aaagacggtc tatgattaaa agctaagatt tttactgacc acacccagga gaaacgatgc
     8941 aagattccat tctgacgacc gtagtgaaag atatcgacgg tgaagtgacc acgctggaga
     9001 agtacgctgg taatgtgctg ttgattgtca atgtcgcctc aaagtgtggc ttaacgccgc
     9061 aatatgagca gttggagaat attcagaaag cctgggccga tcgaggtttt gtggtgctgg
     9121 gattcccgtg caaccagttt ctggaacaag aaccgggcag cgatgaagag attaaaactt
     9181 actgtaccac cacatggggg gtgacgttcc cgatgttcag taagattgaa gttaatggcg
     9241 aaggacgcca tccgctgtat caaaaattga ttgccgcagc gccgaccgca gtcgcgccgg
     9301 aagagagcgg attctatgcc cgtatggtca gcaaaggtcg tgcaccgctg taccctgatg
     9361 atattttatg gaattttgag aaatttttag ttggcaggga cgggttggtc atccagcgtt
     9421 tttccccgga tatgacgcct gaagatccca ttgtgatgga aagcattaaa ctggcgttgg
     9481 caaaataatg tctattgtga tgcagttaca ggatgttgcg gaaactaccc gcctggggcc
     9541 gctttctggc gaggttcggg ctggggagat cctgcacctg gtggggccga atggcgcggg
     9601 taagagtacc ttgcttgcac gaatggccgg aatgaccagc ggtaagggaa gcattcagtt
     9661 cgcggggcaa ccactggaag catggtccgc aacaaaactc gcgctgcatc gcgcctacct
     9721 ttcacaacag cagacgccgc cgtttgcaat gccggtctgg cactacctga cactgcatca
     9781 gcacgataaa acgcgtaccg aactactgaa tgatgtcgca ggggcgctgg ctcttgatga
     9841 caaactcgga cgtagcacca atcaactttc cggcggtgaa tggcaacgcg tacgtcttgc
     9901 tgcggtggtg ctgcaaatca caccacaagc caatcccgca ggtcaattgc tgcttcttga
     9961 tgagccgatg aacagtcttg atgttgcgca acaaagtgcg ttagacaaaa ttctgagcgc
    10021 tctgtgtcag caaggactgg cgattgtgat gagcagtcac gatctcaacc acacattgcg
    10081 tcatgcgcat cgggcgtggt tgctaaaagg tggaaaaatg ctggccagtg gacgcagaga
    10141 agaggtgctc acgccgccaa atctggcgca ggcctatggg atgaattttc gccgtctgga
    10201 tatcgaaggt cacagaatgc tgatttcgac catctgattt cattagtatc ttgaaaatcg
    10261 ggtaattacg acgctaaatt aatgcaagaa taaaaaacag aggaaccgta cggaatgcgt
    10321 ttctgcctta ttttgatcac agcactgttt ctggccgggt gtagccacca taaagcaccg
    10381 ccgccaaatg ccagactttc tgattcgatt accgttattg ccggtttgaa cgaccagcta
    10441 caaagctggc atggcacgcc gtatcgttat ggtggcatga cgcggcgcgg tgtggactgt
    10501 tcgggatttg tggttgtaac gatgcgcgat cgtttcgatt tgcagctgcc ccgagaaacc
    10561 aaagaacaag cctctatcgg cacgcaaatt gataaagacg agttgctgcc tggtgacctg
    10621 gtctttttca aaacgggttc cggacaaaat ggtttgcatg taggtattta cgataccaac
    10681 aaccaattta tccacgcctc taccagcaag ggagtgatgc gttcctcact tgataatgtc
    10741 tattggcaga aaaatttctg gcaggcgaga cgaatttagt atgtcgaaaa atggacggcg
    10801 atgatacatc gccgtccatt tactcctgaa tacagtagta ttttgcagac aaaaattccg
    10861 taaatggatt attcgtttat aatcaggata atctaaaaaa aggacccctg aaaaagtggt
    10921 taacatggaa caaatgaaag ttactcctta tgtaattgtt atgtcttatg gcctcatccc
    10981 ttttagccgg atactgaaaa acatccttcg agagggacgg ttaccatgaa gatttttttg
    11041 gagaatcttt atcattctga ttgctatttc ctgccgataa gagataacca gcaagacctt
    11101 gtcggcgttg agttaattac ccatttctcc agtgaagatg gtacggttcg tatcccgacg
    11161 agtcgggtta tagcgcaact taccgaggag caacattggc aattattttc agagcagttg
    11221 gaattactaa aatcatgcca acattttttt attcaacata aactctttgc ctggcttaat
    11281 ttaacgccac aagtggctac attattatta gaga
//
LOCUS       DAZKHF020000145        11276 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2201S31241
            SAMN43267800-rid25106413.denovo.146, whole genome shotgun sequence.
ACCESSION   DAZKHF020000145 DAZKHF020000000
VERSION     DAZKHF020000145.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267800
            Sequence Read Archive: SRR30307263
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 11276)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11276)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 11276)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350581.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350581.2
            Genome Coverage       :: 151x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:21:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,652
            CDSs (total)                      :: 5,557
            Genes (coding)                    :: 5,320
            CDSs (with protein)               :: 5,320
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 237
            CDSs (without protein)            :: 237
            Pseudo Genes (ambiguous residues) :: 0 of 237
            Pseudo Genes (frameshifted)       :: 102 of 237
            Pseudo Genes (incomplete)         :: 130 of 237
            Pseudo Genes (internal stop)      :: 52 of 237
            Pseudo Genes (multiple problems)  :: 41 of 237
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11276
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267800-rid25106413.denovo.146"
                     /serotype="O 26:H11"
                     /isolate="2201S31241"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-01"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            <1..903
                     /locus_tag="ACHOSC_RS15485"
                     /old_locus_tag="pgaptmp_003097"
     CDS             <1..903
                     /locus_tag="ACHOSC_RS15485"
                     /old_locus_tag="pgaptmp_003097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000420980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ISAs1-like element ISEc1 family transposase"
                     /protein_id="HGU4548994.1"
                     /translation="SCISPAKFHECFINWMRDCHSSNDKDVIAIDGKTLRHSYDKSRR
                     RGAIHVISAFSTMHSLVIGQIKTDEKSNEITAIPELLNMLDIKGKIITTDAMGCQKDI
                     AEKIQKQGGDYLFAVKGNQGRLNKAFEGKFPLKELNNPEHDSYAISEKSHGREEIRLH
                     IVCDVPDELIDFTFEWKGLKKLCVAVSFRSIIAEQKKEPEMTVRYYISSADLTAEKFA
                     TAIRNHWHVENKLHWRLDVVMNEDDCKIRRGNAAELFSGIRHIAINILTNDKVFKAGL
                     RRKMRKAAMDRNYLASVLAGSGLS"
     gene            1172..3409
                     /gene="nrfB"
                     /locus_tag="ACHOSC_RS15490"
                     /old_locus_tag="pgaptmp_003098"
     CDS             1172..3409
                     /gene="nrfB"
                     /locus_tag="ACHOSC_RS15490"
                     /old_locus_tag="pgaptmp_003098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic di-3',5'-guanylate-activated
                     glycosyltransferase NrfB"
                     /protein_id="HGU4548995.1"
                     /translation="MDWLLDVFATWLYGLKVIAITLAVIMFISGLDDFFIDVVYWVRR
                     IKRKLSVYRRYPRMSYRELYKPDEKPLAIMVPAWNETGVIGNMAELAATTLDYENYHI
                     FVGTYPNDPDTQRDVDEVCARFPNVHKVVCARPGPTSKADCLNNVLDAITQFERSANF
                     AFAGFILHDAEDVISPMELRLFNYLVERKDLIQIPVYPFEREWTHFTSMTYIDEFSEL
                     HGKDVPVREALAGQVPSAGVGTCFSRRAVTALLADGDGIAFDVQSLTEDYDIGFRLKE
                     KGMTEIFVRFPVVDEAKEREQRKFLQHARTSNMICVREYFPDTFSTAVRQKSRWIIGI
                     VFQGFKTHKWTSSLTLNYFLWRDRKGAISNFVSFLAMLVMLQLLLLLAYESLWPDAWH
                     FLSIFSGSAWLMTLLWLNFGLMFNRIVQRVIFVTGYYGLTQGLLSVLRLFWGNLINFM
                     ANWRALKQVLQHGDPRRVAWDKTTHDFPSVTGDTRSLRPLGQILLENQVITEEQLDTA
                     LRNRVEGLRLGGSMLMQGLISAEQLAQALAEQNGVAWESIDAWQIPSSLIAEMPASVA
                     LHYAVLPLRLENDELIVGSEDGIDPVSLAALTRKVGRKVRYVIVLRGQIVTGLRHWYA
                     RRRGHDPRAMLYNAVQHQWLTEQQTGEIWRQYVPHQFLFAEILTTLGHINRSAINVLL
                     LRHERSSLPLGKFLVTEGVISQETLDRVLTIQRELQVSMQSLLLKAGLNTEQVAQLES
                     ENEGE"
     gene            3396..6368
                     /gene="nfrA"
                     /locus_tag="ACHOSC_RS15495"
                     /old_locus_tag="pgaptmp_003099"
     CDS             3396..6368
                     /gene="nfrA"
                     /locus_tag="ACHOSC_RS15495"
                     /old_locus_tag="pgaptmp_003099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriophage adsorption protein NfrA"
                     /protein_id="HGU4548996.1"
                     /translation="MKENNLNRVIGWSGLLLTSLLSTSALADNIGTSAEELGLSDYRH
                     FVIYPRLDKALKAQKNNDEATAIREFEYIHQQVPDNIPLTLYLAEAYRHFGHDDRARL
                     LLEDQLKRHPGDARLERSLAAIPVEVKSVTTVEELLAQQKACDAAPTLRCRSEVGQNA
                     LRLAQLPVARAQLNDATFAASPEGKTLRTDLLQRAIYLKQWSQADTLYNEARQQNTLS
                     AAERRQWFDVLLAGQLDDRILALQSQGIFTDPQSYITYATALAYRGEKARLQHYLIEN
                     KPLFTTDAQEKSWLYLLSKYSANPVQALANYTVQFADNRQYVVGATLPVLLKEGQYDA
                     AQKLLATLPANEMLEERYAVSVATRNKAESLRLARLLYQQEPANLTRLDQLTWQLMQN
                     EQSRKAADLLLQRYPFQGDARVSQTLMARLASLLESHPYLATPAKVAILSKPLPLAAQ
                     RQWQSQLPGIADNCPAIVRLLGDMSPSYDAAAWNRLAKCYRDTLPGVALYAWLQAEQR
                     QPNAWQHRAVAYQAYQVEDYATALAAWQKISLHDMSNEDLLAAANTAQAAGNGAARDR
                     WLQQAEQRGLGNNALYWWLHAQRYIPGQPELALSDLTRSINIAPSANAYVARATIYRQ
                     RHNVPAAVSDLRAALELEPNNSNIQAALGYALWDSGDIAQSREMLEQAHKGLPDDPAL
                     IRQLAYVNQRLDDMPATQHYARLVIDDIDNQALITPLTPEQNQQRFNFRRLHEEVGRR
                     WTFSFDSSIGLRSGAMSTANNNVGGAAPGKSYRSYGQLEAEYRIGRNMLLEGDLLSVY
                     SRVFADTGENGVMMPVKNPMSGTGLRWKPLRDQIFFLAVEQQLPLNGQNGASDTMLRA
                     SASFFNGGKYSDEWHPNGSGWFAQNLYLDAAQYIRQDIQAWTADYRVSWHQKVANGQT
                     IEPYAHVQDNGYRDKGTQGAQLGGVGVRWNIWTGETHYDAWPHKVSLGVEYQHTFKAI
                     NQRNGERNNAFLTIGVHW"
     gene            6369..7259
                     /locus_tag="ACHOSC_RS15500"
                     /old_locus_tag="pgaptmp_003100"
     CDS             6369..7259
                     /locus_tag="ACHOSC_RS15500"
                     /old_locus_tag="pgaptmp_003100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4434 family protein"
                     /protein_id="HGU4548997.1"
                     /translation="MRKFIFVLLTLLLVSPFSFAMKGIIWQPQNRDSQVTDTQWQGLM
                     SQLRLQGFDTLVLQWTRYGDAFTQPEQRALLFKRAAAAQQAGLKLIVGLNADPEFFMH
                     QKQSSAALESYLNRLLAADLQQARLWSAAPGVTPDGWYISAEIDDLNWRSEAARQPLL
                     TWLNNAQRLISDVSAKPVYISSFFAGNMSPDGYRQLLEQVKATGVNVWVQDGSGVDKL
                     TAEQRERYLQASADCQSPAPASGIVYELFVAGKGKTFTAKPKPDAEIASLLAKRSSCG
                     KDTLYFSLRYLPVAHGILEY"
     gene            7442..8203
                     /gene="envY"
                     /locus_tag="ACHOSC_RS15505"
                     /old_locus_tag="pgaptmp_003101"
     CDS             7442..8203
                     /gene="envY"
                     /locus_tag="ACHOSC_RS15505"
                     /old_locus_tag="pgaptmp_003101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator EnvY"
                     /protein_id="HGU4548998.1"
                     /translation="MQLSSSEPCVVILTEKEVEVSVNNHATFTLPKNYLAAFACNNNV
                     IELSTLNHVLIPHINRNIINDYLLFLNKNLTCVKPWSRLATPVIACHSRTPEVFRLAA
                     NHSKQQPSKPCEAELTRALLFTVLSNFLEQSRFIALLMYILRSSVRDSVCRIIQSDIQ
                     HYWNLRIVASSLCLSPSLLKKKLKNENTSYSQIVTECRMRYAVQMLLMDNKNITQVAQ
                     LCGYSSTSYFISVFKAFYGLTPLNYLAKQRQKVMW"
     gene            8716..9669
                     /gene="ompT"
                     /locus_tag="ACHOSC_RS15510"
                     /old_locus_tag="pgaptmp_003102"
     CDS             8716..9669
                     /gene="ompT"
                     /locus_tag="ACHOSC_RS15510"
                     /old_locus_tag="pgaptmp_003102"
                     /EC_number="3.4.23.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="omptin family outer membrane protease OmpT"
                     /protein_id="HGU4548999.1"
                     /translation="MRAKLLGIVLTTPIAISSFASTETISFTPDNINADISLGTLSGK
                     TKERVYLAEEGGRKVSQLDWKFNNAAIIKGAINWDLMPQISIGAAGWTTLGSRGGNMV
                     DQDWMDSSNPGTWTDESRHPDTQLNYANEFDLNIKGWLLNEPNYRLGLMAGYQESRYS
                     FTARGGSYIYSSEEGFRDDIGSFPNGERAIGYKQRFKMPYIGLTGSYRYEDFELGGTF
                     KYSGWVEASDNDEHYDPGKRITYRSKVKDQNYYSVAVNAGYYVTPNAKVYVEGAWNRV
                     TNKKGNTSLYDHNDNTSDYSKNGAGIENYNFITTAGLKYTF"
     gene            9856..>11276
                     /locus_tag="ACHOSC_RS15515"
                     /old_locus_tag="pgaptmp_003103"
     CDS             9856..>11276
                     /locus_tag="ACHOSC_RS15515"
                     /old_locus_tag="pgaptmp_003103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HGU4549000.1"
                     /translation="MRKIITHFKVVLTLLLPVTVSAQQIQWQSCMASQFNHWFGEEKP
                     SPDLLCGYLSVPLKYTDTGGDASYEKKSQVKLALTKLPAKSKHKGSILIISGGPGLPG
                     INPYINFDWPVTNLRESWDIIGFDPRGVGQSTPTINCRQSDTETQENITEKQQVLNKI
                     NACIHNTGAEVIRHIGSNEAVYDIDRIRQALGDKQLTAVAYSYGTQIAALYAERFPYN
                     VRSIVLDGVVDIDDLEDNFTWQLKQAQSYQETFDRFASWCARTKSCPLSSDRDKAITQ
                     FHELLSKLHHKPLLDSKRENISSDELISLTTDLLLWRSSWPTLATAIRQFSQGIVSNE
                     IETALSAPIASEESSDASGVILCVDQGDEQLTPEERKSRKDALANAFPAINFDNGRSD
                     SPDFCELWPIHSDLNKTRLKNTVLPSGLLFIAHKYDPTTPWINARKMAEKFSSPLLTI
                     NGDGHTLALTGVNLCVDKAVVHHL"
BASE COUNT         3067 a         2769 c         2728 g         2712 t
ORIGIN      
        1 tcctgtatca gtcctgcaaa atttcacgag tgctttatta actggatgcg tgactgccat
       61 tcatcaaatg ataaagacgt cattgcaatt gatggaaaaa cgctccggca ctcttatgac
      121 aagagtcgcc gcaggggagc gattcatgtc attagtgcgt tctcaacaat gcacagtctg
      181 gtcatcggac agatcaagac ggatgagaaa tctaatgaga ttacagctat cccagaactt
      241 cttaacatgc tggatattaa aggaaaaatc atcacaactg atgcgatggg ttgccagaaa
      301 gatattgcag agaagataca aaaacaggga ggtgattatt tattcgcggt aaaaggaaac
      361 caggggcggc taaataaagc ctttgaggga aaatttccgc tgaaagaatt aaataatcca
      421 gagcatgaca gttacgcaat cagtgaaaag agtcacggca gagaagaaat ccgtcttcat
      481 attgtttgcg atgtccctga tgaacttatt gatttcacgt ttgaatggaa aggtctgaag
      541 aaattatgcg tggcagtctc ctttcggtca ataatagcag aacaaaagaa agagcccgaa
      601 atgacggtca gatattatat cagttctgct gatttaaccg cagagaagtt cgccacagca
      661 atccgaaacc actggcacgt ggagaataag ctgcactggc gtctggacgt ggtaatgaat
      721 gaagacgact gcaaaataag aagaggaaac gccgcagaat tattttcagg gatacggcac
      781 atcgctatta atattttaac gaatgataag gtattcaagg cagggttaag acgtaagatg
      841 cgaaaagcag ccatggacag aaactatctg gcgtcagtcc ttgcggggag cgggctttcg
      901 taatcttgcc ctgcctgctc acctcctcca ggcacttcca ctacattatg acctgctcgg
      961 tgccaccgct ccttttcact atgacgttaa cctcccctcc ttgacaccat gcttttgtct
     1021 acattctacg caaacggcaa cttttataac cagtgtaaaa ataacgtgcc gcaatagtca
     1081 tacctattaa tggtaaaaag ctgtcacaat tcataaaaaa ccttaatata cgccacccta
     1141 aacataacca gcgttaatgt aaggtttttg tgtggactgg cttcttgatg tttttgcaac
     1201 ctggctctac ggcttaaaag taatcgcgat aacgttagcg gtcatcatgt tcatcagcgg
     1261 gctggacgat ttttttattg atgtcgtcta ctgggtacgc cgcattaaac gcaagttgag
     1321 tgtttatcgc cgctacccgc gaatgagtta ccgcgaactg tataaaccag atgaaaaacc
     1381 gttagcgatt atggttccgg cgtggaatga aacgggcgtc atcggcaata tggccgagct
     1441 ggcggcgacc acgctcgact acgaaaacta tcatatcttt gttggcacct accccaacga
     1501 ccccgatact caacgtgatg ttgacgaagt gtgcgctcgc tttccgaacg tgcataaggt
     1561 agtctgcgcg cgtcctggtc ccaccagcaa agccgactgt ctgaacaacg tgctggacgc
     1621 catcacccag tttgaacgta gcgccaattt cgcttttgct ggttttattc tgcatgacgc
     1681 cgaagatgtg atttcaccga tggaattgcg tctgttcaac tatctggtcg agcgtaaaga
     1741 tctgattcag atcccggtgt atccgttcga acgcgaatgg acgcacttca ccagcatgac
     1801 ttacattgat gagttttcag agctgcatgg caaagatgtt ccggtgcgtg aagccctcgc
     1861 cggacaagtg cccagcgcag gcgtcggcac ctgtttcagc cgccgcgccg tgaccgctct
     1921 gttagctgac ggtgacggta ttgctttcga cgtgcagagt ctgactgaag attacgacat
     1981 tggtttccgc ctgaaagaga aaggtatgac ggaaattttt gtccgttttc cggtggtgga
     2041 cgaagccaaa gaacgcgagc agcgtaaatt tttacagcac gcacggacgt caaacatgat
     2101 ctgcgtgcgc gaatatttcc ccgatacctt ttcgactgca gttcgacaaa aatctcgctg
     2161 gatcatcggc attgttttcc agggctttaa aacccacaaa tggacctcca gcctgacgct
     2221 gaactacttt ctctggcgcg accgcaaagg ggcaatcagt aactttgtca gcttcctcgc
     2281 gatgctggtg atgctccagc ttttgctgtt gctggcgtat gaaagtttgt ggcccgatgc
     2341 ctggcatttc ctttctattt ttagcggcag cgcatggtta atgaccctgc tgtggctaaa
     2401 ctttggcttg atgtttaacc gcatcgtgca gcgggtgatt ttcgtcactg gctactacgg
     2461 tctgacgcag gggctgctat ctgtcctgcg tcttttctgg ggcaacctga ttaacttcat
     2521 ggccaactgg cgcgcgctaa aacaggtact tcaacacggc gatccacgtc gcgtcgcgtg
     2581 ggataaaaca acgcatgact tccccagcgt gactggcgat acccgctcgt tgcgcccgtt
     2641 aggccaaatt ctgctggaaa atcaggtcat cactgaagaa caactcgata cagcactgcg
     2701 taatcgcgtc gaaggtctac gcctgggcgg ttcaatgctg atgcaggggc tgattagcgc
     2761 cgagcagctg gcacaggcgc tggcagagca aaacggcgtg gcgtgggaat ccatcgatgc
     2821 ctggcagatc ccgtcctcgc tgattgccga aatgccagcc tccgtagcgc tgcattatgc
     2881 agtactgccg ctgcgtctgg aaaatgatga gttaattgtc ggcagtgaag atggtattga
     2941 ccctgtttcg ctggcggccc tgacgcgtaa agtcggacgc aaagtgcgtt acgtcattgt
     3001 tctgcgggga caaattgtca cggggttacg tcactggtat gcacgccgac gcggtcacga
     3061 tccgcgggca atgttgtaca atgcggttca gcatcagtgg ctcacggaac agcagaccgg
     3121 tgaaatctgg cggcaatatg tgccgcatca gttcctgttc gccgaaatac tgaccacgct
     3181 cggtcatatt aatcgttcag caattaacgt gttgttattg cgccatgaac gcagttctct
     3241 gccgctcggc aagtttttgg tcaccgaagg cgttatcagc caggaaacgt tggatcgcgt
     3301 cctgacaatt caacgcgaat tacaagtttc gatgcaatca ctattactca aagcaggttt
     3361 aaacacagaa caggttgcgc aactggagtc cgaaaatgaa ggagaataac cttaatcgcg
     3421 tcatcggatg gtctggttta ctgctgacgt ctttattgag taccagcgca ctcgcagaca
     3481 atatcggcac cagcgcagaa gagctggggc tgagcgatta tcgccatttt gttatttatc
     3541 cccgtctcga taaggcgctg aaggcacaga aaaataacga cgaagcaacc gccatccgcg
     3601 aatttgaata tatacaccag caggtgccgg ataatattcc gctgacttta taccttgcgg
     3661 aagcctatcg ccattttggt catgatgacc gggcgcggct gttgctggag gatcaactga
     3721 aacgtcaccc aggagatgcc cgacttgagc gcagtctggc ggctattcca gttgaagtga
     3781 aaagcgttac gactgttgaa gaactgcttg cccagcaaaa agcgtgcgat gctgcgccga
     3841 ccctgcgttg tcgcagtgaa gtcgggcaga atgccctgcg gctggcacag ttacctgtcg
     3901 ccagagcgca actgaacgat gcgacgtttg ctgcatcgcc ggaaggaaaa acgctgcgaa
     3961 ccgatctgct gcaacgggca atctacctga aacaatggtc ccaggcagat acgctataca
     4021 atgaagcacg ccagcagaac acattaagcg cggcagaacg ccgtcagtgg tttgacgtgc
     4081 ttcttgccgg gcagctggac gatcggatcc tggcactgca atcacagggg atcttcaccg
     4141 atcctcagtc atatattact tacgcgaccg cgctggctta tcgtggcgaa aaagcacgcc
     4201 tccagcatta tctcattgaa aataagccgc tgtttaccac ggacgcacaa gagaaaagtt
     4261 ggctctatct gttatctaaa tacagcgcca accccgttca ggcattggcg aattatacgg
     4321 tgcagtttgc cgataaccgc cagtatgttg ttggcgcgac gctaccggtg ctgttaaaag
     4381 aaggtcagta cgacgcagcg caaaaactgc tcgccaccct ccccgccaat gaaatgcttg
     4441 aggagcgtta tgctgtcagc gtggcgaccc gtaacaaggc tgaatctctg cgtctggcac
     4501 gattgctgta tcagcaagaa ccggcaaatc ttacccgcct agatcaactg acctggcaac
     4561 tgatgcagaa cgagcagtca cgcaaagctg ccgatttatt gctgcaacgc tatcctttcc
     4621 agggcgatgc gcgtgtcagc cagactttaa tggcgcgact ggcgtctctg ctggaaagtc
     4681 atccttacct ggcaactcca gcgaaggtgg cgattttatc aaaacccttg ccgctggcgg
     4741 cgcaacgtca gtggcaaagc cagttgccgg gtattgcaga taattgcccg gcaatagttc
     4801 gcttgctggg cgatatgtcg ccttcctacg atgccgccgc ctggaaccgt ctggcaaagt
     4861 gttatcggga cacgctaccc ggtgtggcct tgtatgcatg gcttcaggcc gaacaacgac
     4921 aaccgaacgc ctggcaacat cgtgcggtag cctatcaggc gtatcaggtt gaggactacg
     4981 ccaccgcact ggcggcctgg cagaaaatca gtcttcacga catgagcaat gaggatctgc
     5041 ttgctgctgc caataccgcc caggcggcag gaaatggtgc agctcgcgat cgctggctac
     5101 aacaggcaga acaacgtgga ctgggaaaca atgccctcta ctggtggctg catgcgcaac
     5161 gttacattcc tggtcagccg gaactcgcac tgagcgatct cacgcgctca atcaatattg
     5221 cgccttctgc caacgcttac gttgcgcggg cgacaattta tcgccaacgt cataatgtcc
     5281 cggcggcggt gagtgatttg cgcgccgcgc tggaactgga accgaataat agcaacatcc
     5341 aggcagcgct tggttacgcc ttgtgggata gcggtgatat cgcacagtcg cgggaaatgc
     5401 tcgaacaggc gcataaaggg cttccggacg atccggcact gatccgacaa ctggcctacg
     5461 tgaaccagcg tctggatgac atgcctgcga cgcagcacta cgcccggctg gtgattgatg
     5521 acattgataa tcaggcgctg ataaccccac tgaccccaga gcaaaatcag caacgcttca
     5581 atttccgccg tttgcatgag gaggtcggtc gccgctggac gttcagtttc gattcttcta
     5641 tcggcttgcg ttccggggca atgagcaccg ctaacaataa tgtcggcggc gcagcgccag
     5701 ggaaaagcta tcgtagctac gggcaactgg aagccgagta ccgcatcgga cgcaatatgc
     5761 tgctggaagg cgacctgctc tcagtttata gccgcgtctt tgccgatacc ggagaaaacg
     5821 gggtgatgat gccggtgaaa aatccgatgt ccggcaccgg tctgcgctgg aagccgctgc
     5881 gcgatcagat ctttttcctc gccgtcgaac agcagttgcc gctgaacggc caaaatggcg
     5941 catccgatac catgctgcgc gccagcgcct cattctttaa tggcggcaaa tacagcgacg
     6001 aatggcaccc gaacggttca ggctggtttg cccaaaacct gtacctcgat gcggcgcaat
     6061 atatccgcca ggatattcag gcgtggacgg cagattatcg cgtcagctgg catcagaagg
     6121 tagctaacgg acagactatt gagccttacg ctcacgttca ggacaacggc tatcgtgata
     6181 aaggcactca gggcgcgcag cttggcggag tcggggtccg ctggaatatc tggaccggcg
     6241 agacgcacta cgacgcctgg ccgcacaaag tcagtctcgg cgtcgagtat caacatacct
     6301 ttaaggcgat taatcaacgt aacggagagc gcaacaacgc gtttctcacc attggagtgc
     6361 actggtaaat gcgtaagttc attttcgtat tgctgacact gcttttggtc agcccttttt
     6421 cctttgcgat gaaaggtatt atctggcaac cacaaaaccg agatagtcag gttaccgata
     6481 cccagtggca ggggctgatg agtcagttac gtttgcaagg cttcgatacc ctggttttgc
     6541 aatggacccg ttacggcgat gcattcaccc agccagaaca gcgcgcgtta ttgtttaagc
     6601 gtgccgcagc tgcgcaacag gctggcctga agctgattgt cgggctgaac gccgatccgg
     6661 aattttttat gcaccagaaa cagtcgtccg cagcgctgga aagctatctt aatcgcctgc
     6721 tggctgccga tctccagcaa gccagattat ggagcgccgc gcctggcgta acgccggatg
     6781 gctggtacat cagcgcggaa attgacgacc tgaactggcg cagcgaagct gcccgtcagc
     6841 ctttgctaac atggttaaac aacgcgcagc ggctgattag cgatgtttca gcaaaaccgg
     6901 tttatatcag tagttttttc gccggaaaca tgtcgcccga tggctatcgc caactgctgg
     6961 aacaagttaa agcaaccggc gttaatgtct gggtacagga tggcagcggc gtggataaac
     7021 ttaccgctga acagcgtgaa cgttatctac aggccagcgc cgattgccaa agtcccgccc
     7081 ctgccagcgg cattgtttat gaactgtttg tcgccggcaa aggcaaaacc tttacagcga
     7141 aaccgaaacc ggatgcagag atcgcctcgc tgttagcgaa acgttcctct tgcggtaaag
     7201 acactctcta tttctctctg cgctatttgc ccgtcgcgca cggcattctc gagtattaaa
     7261 tctcctccag gtaagtcggg tacgacctgg cttacctctt tcgctctcgt cagaattcca
     7321 tcaagattaa ttgctaaaaa gcgtgctaaa gaaagaagat cgttatcggc gaacgggccg
     7381 atcactcatg caggaagcat agatcctttg agcggagttt ctgatatctg aggagtgcga
     7441 aatgcaattg agcagcagtg aaccttgcgt ggtgatcctg accgaaaaag aggtagaggt
     7501 aagcgtcaat aaccatgcta cgtttaccct tccgaaaaac tacctggccg ccttcgcgtg
     7561 caacaataac gtcattgaac tctcaacgtt aaatcacgta ttaatccccc acatcaaccg
     7621 taacatcatc aacgattatc tgttgttttt aaataagaac ttaacctgtg taaagccctg
     7681 gtcgcggctg gcaaccccgg ttatcgcttg tcatagccgt acaccggaag tgttccggct
     7741 agccgccaac cacagcaagc agcaacccag caaaccctgc gaagcggagt tgacgcgcgc
     7801 attacttttt accgtattgt ctaactttct tgagcaatcg cggtttatag ccctactgat
     7861 gtatatctta cgcagcagcg tccgcgacag cgtctgccgc attattcaaa gcgatattca
     7921 gcattactgg aatctgcgaa ttgtcgccag ttcgctatgt ttaagcccca gcctactcaa
     7981 aaagaaatta aaaaacgaaa ataccagcta tagccagatt gtcacagagt gtcgtatgcg
     8041 ttacgccgta cagatgttat tgatggataa caaaaatatc actcaggtgg cgcaattatg
     8101 tggctatagc agcacgtcgt actttatctc tgtttttaag gcgttttacg gcctgacacc
     8161 gttgaattat ctcgccaaac agcgacaaaa agtgatgtgg tgaagggcaa agcggaaacg
     8221 gataagacgg gcataaatga agaagaaatg gcgcgccctg caggattcga acctgcggcc
     8281 cacgacttag aagttcctag aacgacattt taagtcaaca aattaccgcg ccatctctgc
     8341 gctcacacgt cccactacct caaaacatgt aaagccttgc aagccattgt gaggccttat
     8401 gtgtctcagt tttgtccctc ttttttgtac taaaaaacat agcaattgag gataaacctc
     8461 atgctatttt cgcttatatg cctttaaagg catggcgctt aaatagataa aaacaccaca
     8521 aaagcataaa aaaccacaca gtaaaaccgc aacataaaac aataacagat aattaaacca
     8581 aaaacagata gcgcattgtg ataatcattc aatactaaac aaaatataaa cagtggagca
     8641 atatgtgatt gactcattaa gttagatata aaaaatacat attcaatcat taaaacgatt
     8701 gaatggagaa cttttatgcg ggcgaaactt ctgggaatag ttctgacaac ccctattgcg
     8761 atcagctctt ttgcttctac cgagactata tcgtttactc ctgacaacat aaatgcggac
     8821 attagtcttg gaactctgag cggaaaaaca aaagagcgcg tttatctagc tgaagaagga
     8881 ggccgaaaag tcagtcaact cgactggaaa ttcaataacg ctgcaattat taaaggtgca
     8941 attaattggg atttgatgcc ccagatatct attggggctg cgggctggac tactctcggc
     9001 agccgaggtg gcaatatggt cgatcaggac tggatggatt ccagtaaccc cggaacctgg
     9061 acagatgaaa gtaggcaccc tgatacacaa ctcaattatg ccaacgaatt tgatctgaat
     9121 atcaaaggct ggctcctcaa cgaacccaat taccgcctgg gactcatggc cggatatcag
     9181 gaaagccgtt atagctttac agccagaggt ggttcctata tctacagttc tgaggaggga
     9241 ttcagagatg atatcggctc cttcccgaat ggagaaagag caatcggcta caaacaacgt
     9301 tttaaaatgc cctacattgg cttgactgga agttatcgtt atgaagattt tgagctaggt
     9361 ggcacattta aatacagcgg ctgggtggaa gcatctgata acgatgagca ctatgaccca
     9421 ggaaaaagaa tcacttatcg cagtaaagtc aaagaccaaa attactattc tgttgcagtc
     9481 aatgcaggtt attacgtaac acctaacgca aaagtttatg ttgaaggcgc atggaatcgg
     9541 gttacgaata aaaaaggtaa tacttcactt tatgatcaca atgataacac ttcagactac
     9601 agcaaaaatg gtgcaggcat agaaaactat aacttcatca ctactgctgg tcttaagtac
     9661 accttttaac gaagttaacc agattttctc cccggtggta ttgtataacc ccggggaatt
     9721 tgtttacagt tacaagtagg caatatccac taattttgta agcatttaac ctatgtcata
     9781 acgtgtaatc tcttaccgca acacaagacc tcaatatatt caaatcttat ctttattccg
     9841 atattcagag acacaatgcg aaaaataatt actcatttca aagttgtttt aacgttactt
     9901 ctaccagtaa ccgtatctgc ccagcagata cagtggcaat cctgtatggc cagtcaattc
     9961 aaccactggt ttggtgagga aaaaccgtct cctgacttac tatgtggtta tttgtctgtt
    10021 ccattaaaat atacagacac aggcggagat gcttcttatg aaaaaaaatc acaagtcaaa
    10081 ctagcgttga caaaattgcc ggcaaaaagc aagcataaag gaagtatcct gataataagt
    10141 ggtggtcccg ggttaccagg cataaatcct tatattaact ttgactggcc agtcacaaat
    10201 cttcgtgagt catgggatat tattggattt gatcctcgag gcgtcggaca gtccactccg
    10261 acaataaact gccggcaatc agatacagag actcaggaaa acataaccga aaagcaacaa
    10321 gtattaaata aaattaatgc ctgtatccat aataccggag ccgaagtcat tcgccatatc
    10381 ggctctaacg aggctgtata cgatattgat cgtattaggc aagccttggg ggataaacaa
    10441 ctgacagccg tggcgtattc gtatggaact caaattgcag ccttatatgc agaacgtttt
    10501 ccctacaacg taagatctat cgttcttgat ggagtcgtcg atatcgatga cctggaggac
    10561 aacttcacat ggcaactcaa acaggcacag agttatcagg aaacgtttga tcgctttgca
    10621 tcctggtgtg cgcgtacaaa aagttgcccg ctttcttcag acagagataa ggcaataact
    10681 cagttccatg agctattatc aaaattacat cacaaacctt tattagacag taagagagaa
    10741 aatatatctt cagatgaact catatcatta acaacagacc ttctgctatg gcgttcatca
    10801 tggccaaccc ttgcaactgc catacgccag ttctctcagg ggattgtcag taatgaaatt
    10861 gaaactgcgc tcagtgctcc gatagcctca gaagagtcaa gcgatgcttc gggggtaatc
    10921 ctctgtgtag atcaggggga tgagcaatta acaccagaag agcgaaaatc ccgaaaagac
    10981 gctcttgcga atgccttccc ggctattaac tttgacaatg gacgttccga ttcacctgat
    11041 ttttgtgaat tatggccaat acatagcgac ctgaacaaaa ctcgcctgaa aaatactgtt
    11101 ctgccctctg gtttactgtt tatagcacac aaatacgacc caacaacgcc ctggattaat
    11161 gcccgtaaga tggcagagaa attttccagc ccgttactaa caataaatgg tgatgggcat
    11221 acattagctc tcaccggagt taatttatgt gtagataaag cagttgtaca tcacct
//
LOCUS       DAZKHF020000146        11202 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2201S31241
            SAMN43267800-rid25106413.denovo.147, whole genome shotgun sequence.
ACCESSION   DAZKHF020000146 DAZKHF020000000
VERSION     DAZKHF020000146.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267800
            Sequence Read Archive: SRR30307263
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 11202)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11202)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 11202)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350581.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350581.2
            Genome Coverage       :: 151x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:21:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,652
            CDSs (total)                      :: 5,557
            Genes (coding)                    :: 5,320
            CDSs (with protein)               :: 5,320
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 237
            CDSs (without protein)            :: 237
            Pseudo Genes (ambiguous residues) :: 0 of 237
            Pseudo Genes (frameshifted)       :: 102 of 237
            Pseudo Genes (incomplete)         :: 130 of 237
            Pseudo Genes (internal stop)      :: 52 of 237
            Pseudo Genes (multiple problems)  :: 41 of 237
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11202
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267800-rid25106413.denovo.147"
                     /serotype="O 26:H11"
                     /isolate="2201S31241"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-01"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            <1..110
                     /locus_tag="ACHOSC_RS15520"
                     /old_locus_tag="pgaptmp_003104"
     CDS             <1..110
                     /locus_tag="ACHOSC_RS15520"
                     /old_locus_tag="pgaptmp_003104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001417766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HGU4549001.1"
                     /translation="FLLIFFTDYSHFMDRITLPSTDTARGKGKWKIITA"
     gene            89..193
                     /gene="speFL"
                     /locus_tag="ACHOSC_RS15525"
                     /old_locus_tag="pgaptmp_003105"
     CDS             89..193
                     /gene="speFL"
                     /locus_tag="ACHOSC_RS15525"
                     /old_locus_tag="pgaptmp_003105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leader peptide SpeFL"
                     /protein_id="HGU4549002.1"
                     /translation="MENNNRLMPHIRRTTHIMKFAHRNSFDFHFFNAR"
     gene            504..2702
                     /gene="speF"
                     /locus_tag="ACHOSC_RS15530"
                     /old_locus_tag="pgaptmp_003106"
     CDS             504..2702
                     /gene="speF"
                     /locus_tag="ACHOSC_RS15530"
                     /old_locus_tag="pgaptmp_003106"
                     /EC_number="4.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004586 - ornithine decarboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine decarboxylase SpeF"
                     /protein_id="HGU4549003.1"
                     /translation="MSELKIAVSRSCPDCFSTHRACVNIDESNYIDVAAIILSVSDVE
                     RGKLDEIDATGYDIPVFIATENEERVPAEYLPRISGVFEHCESRKEFYGRQLETAASH
                     YETQLRPPFFRALVDYVNQGNSAFDCPGHQGGEFFRRHPAGNQFVEYFGEMLFRSDLC
                     NADVAMGDLLIHEGAPCIAQQHAAKVFNADKTYFVLNGTSSSNKVVLNALLTPGDLVL
                     FDRNNHKSNHHGALLQAGATPVYLETARNPYGFIGGIDAHCFEESYLRELIAEVAPQR
                     AKEARPFRLAVIQLGTYDGTIYNARQVVDKIGHLCDYILFDSAWVGYEQFIPMMADCS
                     PLLLDLNENDPGILVTQSVHKQQAGFSQTSQIHKKDSHIKGQQRYVPHKRMNNAFMMH
                     ASTSPFYPLFAALDINAKMHEGVSGRNMWMDCVVNGINARKLILDNCQHIRPFVPELV
                     DGKPWQSYETAQIAVDLRFFQFVPGEHWHSFEGYAENQYFVDPCKLLLTTPGIDARNG
                     EYEAFGVPATILANFLRENGVVPEKCDLNSILFLLTPAEDMAKLQQLVALLVRFEKLL
                     ESDAPLAEVLPSIYKQHEERYAGYTLRQLCQEMHDLYARHNVKQLQKEMFRKEHFPRV
                     SMNPQEANYAYLRGEVELVRLPDAEGRIAAEGALPYPPGVLCVVPGEIWGGAVLRYFS
                     ALEEGINLLPGFAPELQGVYIEEHDGRKQVWCYVIKPRDAQSTLLKGEKL"
     gene            2699..4018
                     /gene="potE"
                     /locus_tag="ACHOSC_RS15535"
                     /old_locus_tag="pgaptmp_003107"
     CDS             2699..4018
                     /gene="potE"
                     /locus_tag="ACHOSC_RS15535"
                     /old_locus_tag="pgaptmp_003107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015496 - putrescine:ornithine antiporter
                     activity [Evidence IEA];
                     GO_process: GO:0015822 - ornithine transport [Evidence
                     IEA];
                     GO_process: GO:0015847 - putrescine transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putrescine-ornithine antiporter"
                     /protein_id="HGU4549004.1"
                     /translation="MSQAKSNKMGVVQLTILTMVNMMGSGIIMLPTKLAEVGTISIIS
                     WLVTAVGSMALAWAFAKCGMFSRKSGGMGGYAEYAFGKSGNFMANYTYGVSLLIANVA
                     IAISAVGYGTELLGASLSPVQIGLATIGVLWICTVANFGGARITGQISSITVWGVIIP
                     VVGLCIIGWFWFSPTLYVDSWNPHHAPFFSAVGSSIAMTLWAFLGLESACANTDVVEN
                     PERNVPIAVLGGTLGAAVIYIVSTNVIAGIVPNMELANSTAPFGLAFAQMFTPEVGKV
                     IMALMVMSCCGSLLGWQFTIAQVFKSSSDEGYFPKIFSRVTKVDAPVQGMLTIVIIQS
                     GLALMTISPSLNSQFNVLVNLAVVTNIIPYILSMAALVIIQKVANVPPSKAKVANFVA
                     FVGAMYSFYALYSSGEEAMLYGSIVTFLGWTLYGLVSPRFELKNKHG"
     gene            complement(4074..5714)
                     /gene="pgm"
                     /locus_tag="ACHOSC_RS15540"
                     /old_locus_tag="pgaptmp_003108"
     CDS             complement(4074..5714)
                     /gene="pgm"
                     /locus_tag="ACHOSC_RS15540"
                     /old_locus_tag="pgaptmp_003108"
                     /EC_number="5.4.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004614 - phosphoglucomutase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglucomutase
                     (alpha-D-glucose-1,6-bisphosphate-dependent)"
                     /protein_id="HGU4549005.1"
                     /translation="MAIHNRAGQPAQQSDLINVAQLTAQYYVLKPEAGNAEHAVKFGT
                     SGHRGSAARHSFNEPHILAIAQAIAEERAKNGITGPCYVGKDTHALSEPAFISVLEVL
                     AANGVDVIVQENNGFTPTPAVSNAILVHNKKGGPLADGIVITPSHNPPEDGGIKYNPP
                     NGGPADTNVTKVVEDRANALMADGLKGVKRISLDEAMASGHVKEQDLVQPFVEGLADI
                     VDMAAIQKAGLTLGVDPLGGSGIEYWKRIGEYYNLNLTIVNDQVDQTFRFMHLDKDGT
                     IRMDCSSECAMAGLLALRDKFDLAFANDPDYDRHGIVTPAGLMNPNHYLAVAINYLFQ
                     HRPQWGKDVAVGKTLVSSAMIDRVVNALGRKLVEVPVGFKWFVDGLFDGSFGFGGEES
                     AGASFLRFDGTPWSTDKDGIIMCLLAAEITAVTGKNPQEHYNELAKRFGAPSYNRLQA
                     AATSAQKAALSKLSPEMVSASTLAGDPITARLTAAPGNGASIGGLKVMTDNGWFAARP
                     SGTEDAYKIYCESFLGEEHRKQIEKEAVEIVSEVLKNA"
     gene            complement(5740..6285)
                     /gene="seqA"
                     /locus_tag="ACHOSC_RS15545"
                     /old_locus_tag="pgaptmp_003109"
     CDS             complement(5740..6285)
                     /gene="seqA"
                     /locus_tag="ACHOSC_RS15545"
                     /old_locus_tag="pgaptmp_003109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0032297 - negative regulation of
                     DNA-templated DNA replication initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation negative regulator SeqA"
                     /protein_id="HGU4549006.1"
                     /translation="MKTIEVDDELYSYIASHTKHIGESASDILRRMLKFSASSQPAAP
                     VTKEVRVASPAIVEAKPVKTIKDKVRAMRELLLSDEYAEQKRAVNRFMLLLSTLYSLD
                     AQAFAEATESLHGRTRVYFAADEQTLLKNGNQTKPKHVPGTPYWVITNTNTGRKCSMI
                     EHIMQSMQFPAELIEKVRGTI"
     gene            6470..7234
                     /gene="ybfF"
                     /locus_tag="ACHOSC_RS15550"
                     /old_locus_tag="pgaptmp_003110"
     CDS             6470..7234
                     /gene="ybfF"
                     /locus_tag="ACHOSC_RS15550"
                     /old_locus_tag="pgaptmp_003110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase"
                     /protein_id="HGU4549007.1"
                     /translation="MKLNIRAQTAQNQHNNSPIVLVHGLFGSLDNLGVLARDLVNDHN
                     IIQVDMRNHGLSPRDPVMNYPAMAQDLVDTLDAQQIDKATFIGHSMGGKAVMALTALA
                     PDRIDKLVAIDIAPVDYHVRRHDEIFAAINAVSESDAQTRQQAAAIMRQHLNEEGVIQ
                     FLLKSFVDGEWRFNVPVLWDQYPHIVGWEKIPAWDHPALFIPGGNSPYVSEQYRDDLL
                     AQFPQARAHVIAGAGHWVHAEKPDAVLRAIRRYLND"
     gene            7374..7667
                     /gene="ybfE"
                     /locus_tag="ACHOSC_RS15555"
                     /old_locus_tag="pgaptmp_003111"
     CDS             7374..7667
                     /gene="ybfE"
                     /locus_tag="ACHOSC_RS15555"
                     /old_locus_tag="pgaptmp_003111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415211.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LexA regulated protein"
                     /protein_id="HGU4549008.1"
                     /translation="MAKEQTDRTTLDLFAHERRPGRPKTNPLSRDEQLRINKRNQLKR
                     DKVRGLKRVELKLNAEAVEALNELAESRNMSRSELIEEMLMQQLAALRSQGIV"
     gene            7807..8337
                     /gene="fldA"
                     /locus_tag="ACHOSC_RS15560"
                     /old_locus_tag="pgaptmp_003112"
     CDS             7807..8337
                     /gene="fldA"
                     /locus_tag="ACHOSC_RS15560"
                     /old_locus_tag="pgaptmp_003112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin FldA"
                     /protein_id="HGU4549009.1"
                     /translation="MAITGIFFGSDTGNTENIAKMIQKQLGKDVADVHDIAKSSKEDL
                     EAYDILLLGIPTWYYGEAQCDWDDFFPTLEEIDFNGKLVALFGCGDQEDYAEYFCDAL
                     GTIRDIIEPRGATIVGHWPTAGYHFEASKGLADDDHFVGLAIDEDRQPELTAERVEKW
                     VKQISEELHLDEILNA"
     gene            8547..8633
                     /gene="uof"
                     /locus_tag="ACHOSC_RS15565"
                     /old_locus_tag="pgaptmp_003113"
     CDS             8547..8633
                     /gene="uof"
                     /locus_tag="ACHOSC_RS15565"
                     /old_locus_tag="pgaptmp_003113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001491546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fur leader peptide"
                     /protein_id="HGU4549010.1"
                     /translation="MIRIISRANSVTSSNEVNRLVTGQIPHD"
     gene            8626..9072
                     /gene="fur"
                     /locus_tag="ACHOSC_RS15570"
                     /old_locus_tag="pgaptmp_003114"
     CDS             8626..9072
                     /gene="fur"
                     /locus_tag="ACHOSC_RS15570"
                     /old_locus_tag="pgaptmp_003114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferric iron uptake transcriptional regulator"
                     /protein_id="HGU4549011.1"
                     /translation="MTDNNTALKKAGLKVTLPRLKILEVLQEPDNHHVSAEDLYKRLI
                     DMGEEIGLATVYRVLNQFDDAGIVTRHNFEGGKSVFELTQQHHHDHLICLDCGKVIEF
                     SDDSIEARQREIAAKHGIRLTNHSLYLYGHCAEGDCREDEHAHEGK"
     gene            complement(9156..9482)
                     /gene="chiQ"
                     /locus_tag="ACHOSC_RS15575"
                     /old_locus_tag="pgaptmp_003115"
     CDS             complement(9156..9482)
                     /gene="chiQ"
                     /locus_tag="ACHOSC_RS15575"
                     /old_locus_tag="pgaptmp_003115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ChiQ/YbfN family lipoprotein"
                     /protein_id="HGU4549012.1"
                     /translation="MKKLILIAIMASGLVACAQSTAPQEDSRLKEAYSACINTAQGSP
                     EKIEACQSVLNVLKKEKQHQQFADQESVRVLDYQQCLRATQTGNDQAVKADCDKVWQE
                     IRSNNK"
     gene            complement(9532..10938)
                     /gene="chiP"
                     /locus_tag="ACHOSC_RS15580"
                     /old_locus_tag="pgaptmp_003116"
     CDS             complement(9532..10938)
                     /gene="chiP"
                     /locus_tag="ACHOSC_RS15580"
                     /old_locus_tag="pgaptmp_003116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chitoporin"
                     /protein_id="HGU4549013.1"
                     /translation="MRTFSGKRSTLALAIAGVTAMSGFMAMPEARAEGFIDDSTLTGG
                     IYYWQRERDRKDVTDGAKYKTNLSHSTWNANLDFQSGYAADMFGLDIAAFTAIEMAEN
                     GDSSHPNEIAFSKSNKAYDEDWSGDKSGISLYKAAAKFKYGPVWARAGYIQPTGQTLL
                     APHWSFMPGTYQGAEAGANFDYGDAGALSFSYMWTNEYKAPWHLEMDEFYQNDKTTKV
                     DYLHSIGAKYDFKNNFVLEAAFGQAEGYIDQYFAKASYKFDIAGSPLTTSYQFYGTRD
                     KVDDRSVNDLYDGTAWLQALTFGYRAADVVDLRLEGTWVKADGQQGYFLQRMTPTYAS
                     SNGRLDIWWDNRSDFNANGEKAVFFGAMYDLKNWNLPGFAIGASYVYAWDAKPATWQS
                     NPDAYYDKNRTIEESAYSLDAVYTIQDGRAKGTMFKLHFTEYDNHSDIPSWGGGYGNI
                     FQDERDVKFMVIAPFTIF"
BASE COUNT         2714 a         2761 c         2802 g         2925 t
ORIGIN      
        1 gttttttgtt gatcttcttc acagattata gccatttcat ggatagaata actctacctt
       61 caactgacac agcaagaggt aaaggtaaat ggaaaataat aaccgcttaa tgcctcatat
      121 aaggcggaca acccatatca tgaagtttgc ccatcgtaat agttttgact ttcatttctt
      181 taatgcccgc tagtcttctg actaaagggc accccaacgt acaggtctcc ctgactttaa
      241 gcattacagg ttaatacctg tattcctcgg tgctcatata ctgctaaccc ttttaaactc
      301 taaataattc gagtcgcagc acttgcaact tgaggtatga cgagtatagc cagttaccgg
      361 gctggtctgg gttattgcat ctgcaaaaag caaactactg atttatttat cagcggtgga
      421 gctttgcttt ttttccggcg tgatcgattt ctcctttgag aaattgagga cctgctatta
      481 cctgaaataa agagatgaac aaaatgtcag aattaaaaat tgccgttagt cgttcttgcc
      541 cggattgttt ttccactcat cgtgcatgcg tgaatataga cgaaagtaat tatattgacg
      601 ttgccgccat tattttatca gtcagtgatg ttgaacgtgg aaaactcgat gaaatagacg
      661 ctactggcta tgacattcct gtttttattg caacggaaaa tgaagaacgt gtacccgcag
      721 aatatctgcc acgtatttct ggtgtcttcg aacattgcga atcacgtaaa gagttttatg
      781 gacgtcagtt agaaaccgct gccagccatt atgaaacgca actgcgcccg cccttcttcc
      841 gcgcactggt cgattatgtt aatcagggca atagtgcttt tgactgccct ggtcatcagg
      901 ggggtgagtt tttccgtcgc catccggcag gcaatcagtt cgtggaatac tttggcgaaa
      961 tgctcttccg ctctgacttg tgtaatgctg acgtagcaat gggcgatctg ctgattcatg
     1021 aaggtgcgcc atgtattgca caacagcatg cggctaaagt ctttaatgcc gataaaacct
     1081 atttcgtttt aaacggtact tcatcttcta ataaagtggt cttaaacgca ttgctgacgc
     1141 cgggcgatct ggtgttgttt gaccgcaata accataaatc taaccaccac ggtgcgttgc
     1201 tacaggctgg cgcgacaccg gtttatctgg aaaccgcacg taacccgtat ggctttatcg
     1261 gtggcattga tgcgcactgt tttgaagaaa gttacctgcg tgagctgatc gcggaagtcg
     1321 caccgcagcg ggcaaaagag gctcgtcctt tccgcctcgc tgtgattcag ttaggcacct
     1381 acgacggtac gatttataac gcccgccagg tggtggataa aattggtcat ctgtgtgact
     1441 acattctgtt tgactccgca tgggttggct atgagcaatt tatcccgatg atggctgact
     1501 gttcgccgct gttgctggat cttaatgaga acgatccggg tattctggtt acgcaatccg
     1561 tacataaaca gcaggctggt ttttctcaaa cttcacaaat ccataaaaaa gacagccaca
     1621 tcaaagggca acagcgttat gtaccgcaca aacgcatgaa caacgccttt atgatgcatg
     1681 cctccaccag cccgttctat ccgctgtttg ccgcactgga tatcaacgcc aaaatgcatg
     1741 aaggtgtcag cggtcgtaat atgtggatgg attgtgtggt aaatggcatt aatgcccgca
     1801 agctgatcct cgataactgt cagcatattc gtccgttcgt acctgaactg gtggatggta
     1861 aaccctggca gtcgtatgaa acagcgcaaa ttgcggttga tctgcgcttc ttccagtttg
     1921 taccaggtga gcactggcac tcttttgaag gctatgcaga gaatcaatac tttgtcgatc
     1981 catgcaaact gttgctgaca accccaggta ttgatgcacg taacggcgaa tatgaagcgt
     2041 tcggtgtacc cgcgacgatt cttgctaact tcctgcgcga aaatggcgta gtgccggaaa
     2101 aatgcgatct taactccatc ctcttcctgc tgactccggc agaagatatg gccaagcttc
     2161 agcaacttgt tgccctgctg gtacgcttcg aaaaactgct tgagtccgac gcgccattag
     2221 cagaagtgct gccttccatc tacaaacagc atgaagagcg ctacgccggt tataccctgc
     2281 gtcagttgtg tcaagaaatg catgatttgt atgcccgtca caacgtgaaa caactgcaaa
     2341 aagagatgtt ccgtaaggaa cacttcccac gcgtaagcat gaatccgcaa gaagccaact
     2401 acgcctattt acgcggtgaa gtggagctgg ttcgtctgcc ggatgcagaa ggccgtatcg
     2461 ctgccgaagg tgcgcttcct tatcctccgg gtgtgctgtg tgttgttccg ggtgaaatct
     2521 ggggtggtgc tgttttgcgt tacttcagcg ctctggaaga agggatcaac ctgctgccag
     2581 gttttgcacc ggagctgcag ggtgtctata tcgaagaaca tgatggtcgt aagcaagttt
     2641 ggtgctatgt catcaagcct cgtgatgcgc aaagcaccct gttgaaaggg gaaaaattat
     2701 gagtcaggct aaatcgaaca aaatgggcgt cgttcagtta accatactga cgatggtcaa
     2761 catgatgggc tccggtatca tcatgctgcc gacaaagctt gccgaagttg ggacaatttc
     2821 aattatctcc tggctggtga cagccgtagg ctcaatggca ctggcatggg cattcgcaaa
     2881 atgcggtatg ttcagccgta aatcaggcgg tatgggcggt tatgcggagt atgctttcgg
     2941 taaatccggt aactttatgg cgaactatac ctacggcgtc tcgctgctga ttgctaacgt
     3001 cgcgattgct atttcggcgg ttggttacgg caccgaactg ctcggcgcga gtttgtcgcc
     3061 agtgcagatt ggtcttgcga ccatcggggt gctgtggatt tgtaccgtgg ctaactttgg
     3121 tggtgcgcgc attaccgggc aaatcagtag cattaccgtg tggggggtca ttattccggt
     3181 ggtaggcctg tgcattatcg gctggttctg gtttagcccg acgctttacg ttgattcctg
     3241 gaatccgcat catgcaccgt tcttcagtgc agtaggttct tccatcgcta tgacgctgtg
     3301 ggcttttctc ggtctggagt ctgcgtgtgc gaatactgat gtagtggaaa acccggaacg
     3361 taatgtgcca atcgcggtac tcggcggtac gttaggtgcg gcggtgattt atatcgtctc
     3421 caccaacgtg attgcaggga ttgtgcctaa tatggagctg gcaaattcaa cggcaccatt
     3481 tggcctggcc ttcgcacaga tgttcacacc agaagtgggt aaagtcatta tggcgctgat
     3541 ggtgatgtcc tgctgcggtt cgctgcttgg ctggcagttc accattgccc aggtgtttaa
     3601 atcttcatct gatgaaggct acttccctaa aattttctcc cgtgtaacca aagtggatgc
     3661 accggtgcag ggaatgttga ccattgtgat tatccagagc gggttggcgc tgatgacgat
     3721 tagcccgtcg ctaaacagtc agttcaacgt gctggttaac ctggccgtgg tgaccaatat
     3781 cattccgtat attctgtcaa tggcggcatt agtcattatt cagaaggtcg ctaatgtgcc
     3841 cccctcaaaa gcgaaagttg caaactttgt tgcttttgtt ggcgcgatgt atagctttta
     3901 tgcgctgtac tcatccgggg aagaagccat gctgtacggt tccatcgtga ccttccttgg
     3961 ttggacactg tatggtctgg tatcaccacg ctttgaactg aaaaataaac acggttaaaa
     4021 aagcaaagta aaaaagggcg atcttgcgac cgcccttttt ttattaaatg tgtttacgcg
     4081 tttttcagaa cttcgctaac aatctcaacc gcttctttct caatctgctt gcgatgttct
     4141 tcaccgagga agctttcgca gtagatctta tatgcgtctt ccgtgcctga cggacgcgcg
     4201 gcgaaccagc cgttgtcagt catcactttc agaccgccga tagaagcacc attacccggt
     4261 gccgccgtca gacgtgcggt gatcgggtca cctgccaggg tgctggcgct caccatttcc
     4321 ggagacagct tagacaacgc cgctttttgt gcggaagtcg cagcggcctg caaacggttg
     4381 tagctcggcg caccaaagcg ttttgccagt tcgttgtagt gttcctgcgg gttcttaccg
     4441 gtgacagcgg tgatttccgc cgccagcaga cacataatga tgccgtcttt atcggtggac
     4501 cacggcgtgc cgtcgaaacg caggaaggaa gccccggcgc tctcttcgcc gccaaagccg
     4561 aaactgccgt cgaacagacc atcaacaaac catttgaatc ctaccgggac ttctaccagt
     4621 ttacggccca aggcgttgac cacacggtcg atcatcgcag aagaaaccag cgttttaccg
     4681 acagcaacat ctttgcccca ctgcggacga tgctggaaca ggtagttgat cgccaccgcc
     4741 aggtagtggt tcggattcat caaacctgcc ggagtgacga taccgtgacg gtcataatcc
     4801 gggtcgttag caaacgccag atcgaactta tcacgcagtg ccagcaggcc cgccatcgca
     4861 cactcggagg agcagtccat acggatcgtg ccgtctttat caaggtgcat aaagcggaag
     4921 gtttgatcga cctgatcgtt aacgatggtc aggttgaggt tgtaatactc gccaatacgc
     4981 ttccagtatt cgataccgga accgcccagc ggatcaacgc caagcgtcag gcccgctttc
     5041 tgaatcgccg ccatatcaac gatatcggcc agaccttcca caaacggctg caccagatcc
     5101 tgctctttca catgaccgga tgccatcgct tcgtcgagag agatacgctt cacgcctttc
     5161 aggccatcgg ccatcagtgc gttggccctg tcttccacca ctttggtgac gttggtatca
     5221 gccgggccac catttggcgg gttgtacttg ataccaccat cttccggcgg gttatgggac
     5281 ggtgtaatca cgataccgtc tgccagcggg ccaccttttt tattgtgaac caggatggca
     5341 ttggaaacgg caggcgtcgg agtgaatcca ttgttttcct gcacaatgac atcaacgccg
     5401 ttcgctgcca gcacttccag tacggaaatg aacgcaggct cggacagggc gtgagtatct
     5461 ttacccacat agcaagggcc agtgatgcca tttttcgcac gttcttcagc aattgcctga
     5521 gcgatcgcca gaatgtgcgg ctcgttaaag ctgtggcgcg ctgcactgcc acggtgaccg
     5581 gaagtaccga atttcaccgc gtgctccgca ttccctgctt ctggtttcag tacataatat
     5641 tgcgccgtca gttgggcgac gttaatcaaa tcactctgtt gtgcaggttg gcctgcacga
     5701 ttgtggattg ccattgcttt gtcctttgtc tgcaacgttt tagatagttc cgcgaacctt
     5761 ctcaatcaat tccgccggga attgcatcga ctgcatgatg tgttcgatca tgctgcattt
     5821 acggccggtg ttggtgttgg tgatcaccca atacggcgtg cctggcacat gtttcggctt
     5881 ggtctgatta ccatttttca gcagcgtttg ttcatctgcc gcaaagtaaa cgcgtgtacg
     5941 accgtgcaac gattccgttg cttcggcaaa cgcctgggcg tcaagagaat atagtgtaga
     6001 caacagcagc ataaagcgat tgaccgctcg cttttgctct gcgtattcgt ccgaaagcag
     6061 aagttcacgc attgcgcgaa ccttgtcttt aatcgttttg accggcttcg cttcgacgat
     6121 agcaggtgac gcaacgcgaa cctctttcgt caccggagca gcaggctgtg atgaggcgga
     6181 aaatttcaac atacgccgta aaatgtcgga tgcgctctcg ccgatatgct tagtgtggct
     6241 ggcaatatag ctgtagagtt catcatcaac ttcaatcgtt ttcatcttaa tccagtgcag
     6301 tgtcttagct gaatacaagt cgccaggagt atatgaataa atcccggcag cggatagcgt
     6361 caagcctggc ttgcagaaaa actcggaata aaccttattt gttgcagccc tgtggcacaa
     6421 tggtcaacat aataccctaa cccgacaacg cgaaaaaaag aactttgcca tgaaattgaa
     6481 tatccgcgcg caaactgcac aaaaccagca caataattct cccatcgttc ttgtccatgg
     6541 tctgtttggt agcctcgaca accttggcgt actggctcgc gatctggtaa acgatcacaa
     6601 tatcatccag gttgatatgc gtaaccacgg tctttcaccg agagatccgg taatgaatta
     6661 cccggcgatg gcgcaggatc ttgttgatac tctggatgca cagcagatcg acaaagcaac
     6721 atttatcggt cactccatgg gcggtaaagc ggtaatggca cttactgcac tggcccccga
     6781 tcgcatcgat aaactggtgg cgatcgatat cgcaccggtc gactatcacg tacgccgtca
     6841 tgatgagatt ttcgcggcta tcaacgcggt cagtgaatcg gacgcacaaa ctcgccagca
     6901 agcagcagca ataatgcgcc agcatcttaa tgaagaaggg gtgattcagt ttctgttgaa
     6961 atcttttgtt gacggggagt ggcgctttaa cgtgccggta ttgtgggatc agtatccgca
     7021 tattgtgggt tgggagaaaa tcccggcatg ggatcaccct gccctgttta tccctggcgg
     7081 caattctccg tatgttagcg agcagtaccg tgatgattta ctggcacaat ttccacaggc
     7141 acgggcgcat gtgattgcag gcgcgggtca ctgggtccat gctgaaaaac cggatgcggt
     7201 attacgcgct atccgtcgct atctcaatga ttaacgcaac tgattaaaaa cccagcgtcc
     7261 gcgtcgcttt ccgcgcgtgg gcgttggcgt gactcatcgc gctgatgtat tatggcgcgc
     7321 tatccatccg ggcagaggcc tggctgattg tttcccccga agtcaccaag atcatggcca
     7381 aagaacaaac ggaccgtacg acattagatc tgttcgcgca cgagcgtcga ccgggacgac
     7441 cgaaaactaa tccgctttcg cgcgatgaac agctgcgtat taataaacgc aaccagctaa
     7501 aacgcgacaa agtacgtggc cttaagcgtg tcgaactgaa gctgaacgcg gaagctgtcg
     7561 aggcgctgaa cgagctggcg gagtcgcgca atatgagccg tagcgaactg atcgaagaga
     7621 tgttaatgca gcaactggcg gcgctgcgta gtcagggcat cgtttaaatt tccactttca
     7681 tgtagcacag tgtgcagtcc tgctcgtttg ctgattccgc aagactgcct gttctgctat
     7741 gattgccttt atccgtgggc aattttccac ccccatttca ataagtttca agaggttatt
     7801 tcactcatgg ctatcactgg catctttttc ggcagcgaca ccggtaatac cgaaaatatc
     7861 gcaaaaatga ttcaaaaaca gcttggtaaa gacgttgccg atgtccatga cattgcaaaa
     7921 agcagcaaag aagatctgga agcttatgac attctgctgc tgggcatccc aacctggtat
     7981 tacggcgaag cgcagtgtga ctgggatgac ttcttcccga ctctcgaaga gattgatttc
     8041 aacggcaaac tggttgcgct gtttggttgt ggtgaccagg aagattacgc cgaatatttc
     8101 tgcgacgcat tgggcaccat ccgcgacatc attgaaccgc gcggtgcaac catcgttggt
     8161 cactggccaa ctgcgggcta tcatttcgaa gcatcaaaag gtctggcaga tgacgaccac
     8221 tttgtcggtc tggctatcga cgaagaccgt cagccggaac tgaccgctga acgtgtagaa
     8281 aaatgggtta aacagatttc tgaagagttg catctcgacg aaattcttaa tgcctgatgt
     8341 gatgcggcgt agactcatgt ctacgccgta ttaatagata atgccaatca aaataattgc
     8401 tacaaatttg taacttttgc tgttgtacct gtacaatgtc ccggtgttca agtggccttg
     8461 ccgttgtaaa tgtaagctgt gccacgtttt tattaacaat atttgccagg gacttgtggt
     8521 tttcatttag gcgtggcaat tctataatga tacgcattat ctcaagagca aattctgtca
     8581 cttcttctaa tgaagtgaac cgcttagtaa caggacagat tccgcatgac tgataacaat
     8641 accgccctaa agaaagctgg cctgaaagta acgcttccac gtttaaaaat cctggaagtt
     8701 cttcaggagc cggacaacca tcacgtcagt gcggaagatt tatacaaacg tctgatcgat
     8761 atgggtgaag aaattggtct ggctacggta tatcgcgtac tgaaccagtt tgacgacgct
     8821 ggtatcgtca cccgccacaa ttttgaaggc ggtaaatccg tatttgaact gacacagcaa
     8881 catcaccacg atcacctgat ttgcctcgac tgcggcaagg ttatcgaatt tagtgatgat
     8941 tccatcgaag cgcgtcagcg tgaaattgcc gcaaaacatg gcattcgcct gactaaccac
     9001 agtctctatc tttacggtca ctgtgccgaa ggcgattgcc gcgaagatga gcacgcgcac
     9061 gaaggcaaat aagccagcct gaacgagaaa agccaacctg cgggttggct tttttataca
     9121 agggaaataa gaaaagcgag agttacagct ctcacttatt tgttattact gcgtatttcc
     9181 tgccagacct tatcacaatc ggctttcacc gcctgatcat ttccggtttg cgttgcacgc
     9241 agacactgct gataatccag tacgcgtaca ctttcctggt cggcaaactg ctgatgttgt
     9301 ttctctttct tcagcacgtt taacacgctc tggcaggctt caattttttc tggcgaacct
     9361 tgtgcggtgt tgatacaggc gctgtatgcc tctttcagac gactgtcttc ctgtggcgcg
     9421 gttgattgcg cacaagctac cagccccgat gccattatgg cgatgagaat cagttttttc
     9481 atagcaaatt cctcaaacgt gcggcaggca caaaacctgc cgcgtcgggc atcagaagat
     9541 ggtgaatggt gcgattacca taaatttcac gtcacgctca tcctggaaga tgttaccgta
     9601 accgccgccc cagcttggga tgtcagagtg gttgtcgtat tcggtgaagt gcagtttgaa
     9661 catcgtgcct ttggcgcgac cgtcctgaat ggtgtagacc gcatccaggc tgtaggcaga
     9721 ctcttcaata gtccggtttt tgtcgtagta cgcatccgga ttgctctgcc aggtcgcagg
     9781 tttagcatcc catgcgtaaa cgtaggaagc gccgatggcg aagcctggaa gattccagtt
     9841 tttcaggtca tacatcgcac cgaagaagac cgctttttcg ccgttggcgt tgaagtcgga
     9901 acggttatcc caccagatat ccaggcggcc gttggaggaa gcgtaggttg gagtcatacg
     9961 ttgcaggaag tatccctgct gaccgtcagc cttaacccag gtgccttcga ggcgcaaatc
    10021 cactacgtca gccgcccggt aaccaaaggt caacgcttgc agccaggcgg tgccgtcata
    10081 aaggtcgttg acgctgcgat cgtcaacttt atcgcgcgta ccatagaact ggtagctggt
    10141 ggttaacggg ctaccggcga tatcaaattt gtagctggct ttggcaaaat attgatcgat
    10201 atacccttcc gcctgaccaa atgccgcttc cagtacgaag ttatttttga agtcgtattt
    10261 cgcaccgatg gagtgcaggt aatcaacttt ggtggtttta tcgttctgat aaaactcatc
    10321 catttccaga tgccacggcg ctttgtattc gttggtccac atgtaggaga aactcaaggc
    10381 accagcatcg ccgtaatcaa aattcgcccc ggcttccgcc ccctgataag tacctggcat
    10441 aaagctccag tgcggcgcta acagcgtttg accagttggc tgaatgtaac ctgccctcgc
    10501 ccaaaccgga ccgtatttaa atttggccgc agctttatac aggcttatac cgcttttgtc
    10561 gccggaccag tcttcgtcat aggctttatt actttttgaa aacgcgattt cgttcgggtg
    10621 ggagctgtcg ccgttttccg ccatttcaat cgccgtaaac gcagcaatat caaggccgaa
    10681 catatcagca gcataaccgg actgaaaatc gaggttggca ttccaggtgg agtgagaaag
    10741 gttggttttg tatttggcgc cgtcggtaac atctttacgg tcgcgttcac gctgccagta
    10801 atatataccg ccggttaagg ttgaatcgtc gatgaatcct tcggcgcgag cctccggcat
    10861 tgccataaag cccgacattg ctgtaacacc ggcgatagcc agcgccagcg tactacgttt
    10921 gccactaaac gtacgcatgg gttaatcctc tttgacgtat aaattgctgc accaaaggtg
    10981 aacagcgaaa aaataaaaat taaaaaagta gcgttgagaa aagtctcgct gactacagga
    11041 acagactatt tttcgcgacg cgaattatca atctctttct gtaaccagaa tgtaagaaca
    11101 tgagcaaccg cacatatttt gatgattttt gttacaaggc ttaagtttgc ggttgaaagg
    11161 cttgcagaag aataaaagcg tgaatttcca gcaagatgaa ag
//
LOCUS       DAZKHF020000147        11112 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2201S31241
            SAMN43267800-rid25106413.denovo.148, whole genome shotgun sequence.
ACCESSION   DAZKHF020000147 DAZKHF020000000
VERSION     DAZKHF020000147.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267800
            Sequence Read Archive: SRR30307263
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 11112)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11112)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 11112)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350581.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350581.2
            Genome Coverage       :: 151x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:21:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,652
            CDSs (total)                      :: 5,557
            Genes (coding)                    :: 5,320
            CDSs (with protein)               :: 5,320
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 237
            CDSs (without protein)            :: 237
            Pseudo Genes (ambiguous residues) :: 0 of 237
            Pseudo Genes (frameshifted)       :: 102 of 237
            Pseudo Genes (incomplete)         :: 130 of 237
            Pseudo Genes (internal stop)      :: 52 of 237
            Pseudo Genes (multiple problems)  :: 41 of 237
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11112
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267800-rid25106413.denovo.148"
                     /serotype="O 26:H11"
                     /isolate="2201S31241"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-01"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            complement(<1..871)
                     /gene="yihR"
                     /locus_tag="ACHOSC_RS15585"
                     /old_locus_tag="pgaptmp_003117"
     CDS             complement(<1..871)
                     /gene="yihR"
                     /locus_tag="ACHOSC_RS15585"
                     /old_locus_tag="pgaptmp_003117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709679.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldose-1-epimerase"
                     /protein_id="HGU4549014.1"
                     /translation="MQITNMHCSGQTVSLAAGDYHATIVTVGAGLAELTFQGCHLVIP
                     HKPEEMPLAHLGKVLIPWPNRIANGCYRYQGQEYQLPINEHSSKAAIHGLLAWRDWQI
                     SELTATSVTLTAFLPPSYGYPFMLASQVVYSLNAHTGLSVEIASQNIGTVAAPYGVGI
                     HTYLTCNLTSVDEYLFQLPANQVYAVDEHVNPTTLHHVDELDLNFTQAKTIAATKIDH
                     TFKTANDLWEITITHPQQALSVSLCCDQPWVQIYSGEKLQRQGLAVEPMSCPPNAFNS
                     GIDLLLLEPGKPHR"
     gene            complement(1009..2250)
                     /gene="yihS"
                     /locus_tag="ACHOSC_RS15590"
                     /old_locus_tag="pgaptmp_003118"
     CDS             complement(1009..2250)
                     /gene="yihS"
                     /locus_tag="ACHOSC_RS15590"
                     /old_locus_tag="pgaptmp_003118"
                     /EC_number="5.3.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418316.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfoquinovose isomerase"
                     /protein_id="HGU4549015.1"
                     /translation="MKWFNTLSHNRWLEQETDRIFDFGKNSVVPTGFGWLGNKGQIKE
                     EMGTHLWITARMLHVYSVAAAMGRPGAYSLVDHGIKAMNGALRDKKYGGWYACVNDEG
                     VVDASKQGYQHFFALLGAASAVTTGHPEARKLLDYTIEIIEKYFWSEEEQMCLESWDE
                     AFSKTEEYRGGNANMHAVEAFLIVYDVTHDKKWLDRAIRVASVIIHDVARNNHYRVNE
                     HFDTQWNPLPDYNKDNPAHRFRAFGGTPGHWIEWGRLMLHIHAALEARCEQPPAWLLE
                     DAKGLFNATVRDAWAPDGADGIVYTVDWEGKPVVRERVRWPIVEAMGTAYALYTVTGD
                     RQYETWYQTWWEYCIKYLMDYENGSWWQELDADNKVTTKVWDGKQDIYHLLHCLVIPR
                     IPLAPGMAPAVAAGLLDINAK"
     gene            complement(2267..3145)
                     /gene="yihT"
                     /locus_tag="ACHOSC_RS15595"
                     /old_locus_tag="pgaptmp_003119"
     CDS             complement(2267..3145)
                     /gene="yihT"
                     /locus_tag="ACHOSC_RS15595"
                     /old_locus_tag="pgaptmp_003119"
                     /EC_number="4.1.2.57"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfofructosephosphate aldolase"
                     /protein_id="HGU4549016.1"
                     /translation="MNKYTINDITRASGGFAMLAVDQREAMRMMFAAAGAPAPVADSV
                     LTDFKVNAAKALSPYASAILVDQQFCYRQVVEQNAIAKSCAMIVAADEFIPGNGIPVD
                     SVVIDRKINPLQIKQDGGKALKLLVLWRSDEDAQQRLDMVKEFNELCHSHGLVSIIEP
                     VVRPPRRGDKFDREQAIIDAAKELGDSGADLYKVEMPLYGKGPQQELLCASQRLNDHI
                     NMPWVILSSGVDEKLFPRAVRVAMTAGASGFLAGRAVWASVVGLPDNELMLRDVCAPK
                     LQQLGDIVDEMMAKRR"
     gene            complement(3169..4065)
                     /gene="yihU"
                     /locus_tag="ACHOSC_RS15600"
                     /old_locus_tag="pgaptmp_003120"
     CDS             complement(3169..4065)
                     /gene="yihU"
                     /locus_tag="ACHOSC_RS15600"
                     /old_locus_tag="pgaptmp_003120"
                     /EC_number="1.1.1.373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfolactaldehyde 3-reductase"
                     /protein_id="HGU4549017.1"
                     /translation="MAAIAFIGLGQMGSPMASNLLQQGHQLRVFDVNAEAVRHLVDKG
                     ATPAANPAQAAKDAEFIITMLPNGDLVRNVLFGENGVCEGLSTDALVIDMSTIHPLQT
                     DKLIADMQAKGFSMMDVPVGRTSANAITGTLLLLAGGTAEQVERATPILMAMGSELIN
                     AGGPGMGIRVKLINNYMSIALNALSAEAAVLCEALNLPFDVAVKVMSGTAAGKGHFTT
                     SWPNKVLSGDLSPAFMIDLAHKDLGIALDVANQLHVPMPLGAASREVYSQARAAGRGR
                     QDWSAILEQVRVSAGMTAKVKM"
     gene            4233..5129
                     /gene="yihV"
                     /locus_tag="ACHOSC_RS15605"
                     /old_locus_tag="pgaptmp_003121"
     CDS             4233..5129
                     /gene="yihV"
                     /locus_tag="ACHOSC_RS15605"
                     /old_locus_tag="pgaptmp_003121"
                     /EC_number="2.7.1.184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418319.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfofructose kinase"
                     /protein_id="HGU4549018.1"
                     /translation="MIRVACVGITVMDRIYYVEGLPTESGKYVARNYTEVGGGPAATA
                     AVAAARLGAQVDFIGRVGDDDTGNSLLAELESWGVNTRYTKRYNQAKSSQSAIMVDTK
                     GERIIINYPSPDLLPDAEWLEEIDFSQWDVVLADVRWHDGAKKAFTLARQAGVMTVLD
                     GDITPQDISELVALSDHAAFSEPGLARLTGVKEMASALKQAQTLTNGHVYVTQGSAGC
                     DWLENGGRQHQPAFKVDVVDTTGAGDVFHGALAVALATSGDLAESVRFASGVAALKCT
                     RPGGRAGIPDCDQTRSFLSLFV"
     gene            5163..5948
                     /gene="csqR"
                     /locus_tag="ACHOSC_RS15610"
                     /old_locus_tag="pgaptmp_003122"
     CDS             5163..5948
                     /gene="csqR"
                     /locus_tag="ACHOSC_RS15610"
                     /old_locus_tag="pgaptmp_003122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418320.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR family DNA-binding transcription
                     regulator"
                     /protein_id="HGU4549019.1"
                     /translation="MSLTELTGNPRHDQLLMLIAERGYMNIDELANLLDVSTQTVRRD
                     IRKLSEQGLITRHHGGAGRASSVVNTAFEQREVSQTEEKKAIAEAVADYIPDGSTIFI
                     TIGTTVEHVARALLNHNHLRIITNSLRVAHILYHNPRFEVMVPGGTLRSHNSGIIGPS
                     AASFVADFRADYLVTSVGAIESDGALMEFDVNEANVVKTMMAHARNILLVADHTKYHA
                     SAAVEIGNVAQVTALFTDELPPAALKSRLQDSQIEIILPQEDA"
     gene            6047..6646
                     /gene="yihX"
                     /locus_tag="ACHOSC_RS15615"
                     /old_locus_tag="pgaptmp_003123"
     CDS             6047..6646
                     /gene="yihX"
                     /locus_tag="ACHOSC_RS15615"
                     /old_locus_tag="pgaptmp_003123"
                     /EC_number="3.1.3.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312835.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphatase"
                     /protein_id="HGU4549020.1"
                     /translation="MLYIFDLGNVIVDIDFNRVLGAWSDLTRVPLATLKKSFHMGEAF
                     HQHERGEISDEAFAEALCHEMALPLSYEQFSHGWQAVFVALRPEVIAIMHKLREQGHR
                     VVVLSNTNRLHTTFWPEEYPEIRDAADHIYLSQDLGMRKPEARIYQHVLQAEGFSPSD
                     TVFFDDNADNIEGANQLGITSILVKDKTTIPDYFAKVLC"
     gene            6640..7512
                     /gene="yihY"
                     /locus_tag="ACHOSC_RS15620"
                     /old_locus_tag="pgaptmp_003124"
     CDS             6640..7512
                     /gene="yihY"
                     /locus_tag="ACHOSC_RS15620"
                     /old_locus_tag="pgaptmp_003124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="virulence factor BrkB family protein"
                     /protein_id="HGU4549021.1"
                     /translation="MLKTIQDKARHRTRPLWAWLKLLWQRIDEDNMTTLAGNLAYVSL
                     LSLVPLVAVVFALFAAFPMFSDVSIQLRHFIFANFLPATGDVIQRYIEQFVANSNKMT
                     AVGACGLIVTALLLMYSIDSALNTIWRSKRARPKIYSFAVYWMILTLGPLLAGASLAI
                     SSYLLSLRWASDLNTVIDNVLRIFPLLLSWISFWLLYSIVPTIRVPNRDAIVGAFVAA
                     LLFEAGKKGFALYITMFPSYQLIYGVLAVIPILFVWVYWTWCIVLLGAEITVTLGEYR
                     KLKQAAEQEEDDEP"
     gene            7509..7946
                     /gene="dtd"
                     /locus_tag="ACHOSC_RS15625"
                     /old_locus_tag="pgaptmp_003125"
     CDS             7509..7946
                     /gene="dtd"
                     /locus_tag="ACHOSC_RS15625"
                     /old_locus_tag="pgaptmp_003125"
                     /EC_number="3.1.1.96"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000560980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004045 - aminoacyl-tRNA hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-aminoacyl-tRNA deacylase"
                     /protein_id="HGU4549022.1"
                     /translation="MIALIQRVTRASVTVEGEVTGEIGAGLLVLLGVEKDDDEQKANR
                     LCERVLGYRIFSDAEGKMNLNVQQAGGSVLVVSQFTLAADTERGMRPSFSKGASPDRA
                     EALYDYFVERCRQQEMNTQTGRFAADMQVSLVNDGPVTFWLQV"
     gene            7991..8932
                     /gene="fabY"
                     /locus_tag="ACHOSC_RS15630"
                     /old_locus_tag="pgaptmp_003126"
                     /gene_synonym="yiiD"
     CDS             7991..8932
                     /gene="fabY"
                     /locus_tag="ACHOSC_RS15630"
                     /old_locus_tag="pgaptmp_003126"
                     /gene_synonym="yiiD"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001666856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acid biosynthesis protein FabY"
                     /protein_id="HGU4549023.1"
                     /translation="MYHLRVPQTEEELERYYQFRWEMLRKPLHQPKGSERDAWDAMAH
                     HQMVVDEQGNLVAVGRLYINADNEASIRFMAVHPDVQDKGLGTLMAMTLESVARQEGV
                     KRVTCSAREDAVEFFAKLGFVNQGEITTPTTTPIRHFLMIKPVATLDDILHRGDWCAQ
                     LQQAWYEHIPLSEKMGVRIQQYTGQKFITTMPETGNQNPHHTLFAGSLFSLATLTGWG
                     LIWLMLRERHLGGTIILADAHIRYSKPISGKPHAVADLGALSGDLDRLARGRKARVQM
                     QVEIFGDETPGAVFEGTYIVLPAKPFGPYEEGGNEEE"
     gene            complement(8996..9904)
                     /locus_tag="ACHOSC_RS15635"
                     /old_locus_tag="pgaptmp_003127"
     CDS             complement(8996..9904)
                     /locus_tag="ACHOSC_RS15635"
                     /old_locus_tag="pgaptmp_003127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001296611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HGU4549024.1"
                     /translation="MALEKGIAELVEKFIAAGRPSSRQQSIAQRREGYIASAVLAGET
                     ETRVDIQTIELEGMTLRIVSPLNAPTLLPTIIYYYGGCFVSGGFATHDNQLRQLAYYG
                     QCRVIAVQYRLAPEHTFPAAHDDAQRGAEIVWQHAERLGVDKQRITLAGDSAGGHLAL
                     VTALRLKRAGEWQPAQLILIYPMLDATAHFESYIRNGHDYIITRDTLLSGFEMYLPGI
                     ERRHPEASPLWRDDFNGLPPVHIITAEYDPLCDEGEALYHRMTGQGVQCTCQRYLGVI
                     HGFFQLGGISEAARSALRDVAWRAGR"
     gene            10133..10444
                     /locus_tag="ACHOSC_RS15640"
                     /old_locus_tag="pgaptmp_003128"
     CDS             10133..10444
                     /locus_tag="ACHOSC_RS15640"
                     /old_locus_tag="pgaptmp_003128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000897300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HGU4549025.1"
                     /translation="MLFIETEIFTEDVQKLLTDDEFSRFQFFLALNPDYGEVIPETGG
                     LRKVRWVSGGKGKRAGVRVIYFHQVKHYEIRLLLIYRKGIKDDLSPQEKAMLRLLNTR
                     W"
     gene            10445..10735
                     /gene="nadS"
                     /locus_tag="ACHOSC_RS15645"
                     /old_locus_tag="pgaptmp_003129"
     CDS             10445..10735
                     /gene="nadS"
                     /locus_tag="ACHOSC_RS15645"
                     /old_locus_tag="pgaptmp_003129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000356400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NadS family protein"
                     /protein_id="HGU4549026.1"
                     /translation="MDKALFERLTHSMAQMNEIIEGTRQPSRTFEVDAMKIKEIRRAS
                     GLSQSKFADLISVSVDTLRNWEQGRRSPTGPAKALLRAIANDPQHVLQALNR"
BASE COUNT         2635 a         2766 c         2976 g         2735 t
ORIGIN      
        1 ggcggtgcgg ttttcctggt tccagtaata ataagtcgat acctgaatta aaggcgttag
       61 gcgggcaact cataggttca actgccagtc cctgacgctg taatttttca ccgctataaa
      121 tttgtaccca gggttgatcg caacaaaggc taaccgaaag cgcctgttgt ggatgggtaa
      181 tggttatttc ccacagatca ttagcggttt taaaggtgtg gtcgattttt gtcgcggcta
      241 ttgttttcgc ctgcgtgaaa tttaaatcca gctcatcaac gtggtgaagc gttgtcggat
      301 ttacgtgttc atcaacggca taaacctggt ttgccggaag ctggaacaga tattcatcaa
      361 cagatgttaa gttgcaggtt aaataagtat gaatacccac gccataaggc gctgcgacag
      421 tgccgatatt ctggcttgcg atctcaacgg aaagacccgt gtgtgcgttg agcgaataaa
      481 caacttgtga agccagcata aacggatagc cgtaactggg cggtagaaaa gctgtcaggg
      541 taacgctggt ggcggttaat tcactaattt gccagtctcg ccaggcaagc agcccgtgaa
      601 tagcggcttt tgaactatgt tcattaatgg gtaactgata ttcctgcccc tgatagcggt
      661 aacagccgtt ggcgatccgg ttcggccacg gaataagcac tttacccaga tgcgccagcg
      721 gcatctcttc gggtttgtgc gggatgacta aatgacaacc ctgaaaggtt aattcagcca
      781 gaccagcacc gacggtcaca atggtcgcgt gataatcccc cgcggcaagt gaaacggtct
      841 gtccactgca atgcatattg gtgatttgca tacaaggcac cttaattaac gacatacagc
      901 ctcccgtgag gctgatgtca aacccaaaaa ctgcaccgga cggtcccctc gcccctttgg
      961 ggagagggtt agggtgaggg gaaaacccgc accctatttt cagatcagtt atttcgcatt
     1021 aatatccagc agacccgctg caaccgctgg agccatgccg ggcgctaacg ggatacgcgg
     1081 gatcaccagg caatgcagca ggtgataaat atcctgtttg ccgtcccaga ctttggtggt
     1141 gaccttattg tccgcatcca gctcctgcca ccaggaacca ttttcatagt ccatcaggta
     1201 cttaatgcag tactcccacc atgtttgata ccaggtttca tactggcgat cgccagtgac
     1261 ggtgtagagc gcgtaggccg tacccattgc ttcgacgata ggccaacgta cacgttcgcg
     1321 gaccaccggt tttccttccc agtcaacggt ataaacaatc ccgtccgcgc catcgggtgc
     1381 ccaggcatcg cgcacggtgg cgttaaacag acctttggca tcttctagca gccatgctgg
     1441 tggttgttcg caacgggctt ccagggcagc atggatgtgc agcattaaac ggccccattc
     1501 gatccagtgg cctggtgtac ctccgaacgc gcggaagcga tgcgccgggt tatctttgtt
     1561 gtaatccggc agcggattcc actgggtatc gaagtgttcg ttaacgcgat aatgattatt
     1621 tctggcgacg tcgtggataa tcacggaagc cacgcgaatc gcgcgatcca gccatttttt
     1681 gtcgtgagtg acgtcataaa caatcaagaa agcttccacc gcgtgcatat tggcattgcc
     1741 gccgcggtac tcttcggttt tgctgaaggc ttcgtcccag gattccaggc acatctgctc
     1801 ttcttcgctc cagaaatatt tctcgataat ttcaatggtg taatcgagca gcttgcgcgc
     1861 ttccgggtga cccgttgtga cggcgctggc ggcacccagc agagcaaaga agtgttgata
     1921 gccctgtttg gaggcatcca ccacgccctc gtcatttacg caggcatacc agccgccata
     1981 ttttttatca cgcagtgcgc cgttcatggc tttgataccg tgatcaacca acgagtaagc
     2041 gccaggtcga cccatcgccg cagcaacgga ataaacgtgt aacatacgag cggtgatcca
     2101 cagatgggtg cccatctctt ctttgatttg ccctttattg cctaaccagc caaaaccagt
     2161 cggcactact gaatttttac caaaatcaaa gatgcggtcg gtttcctgtt ccagccaacg
     2221 gttgtggctt agggtgttaa accatttcat tccttttatc ctcatcttag cggcgtttag
     2281 ccatcatttc gtcgacgata tcgccaagtt gttgtaattt cggtgcgcaa acgtcacgca
     2341 gcatcagctc gttgtctggt aaaccgacga ccgatgccca gactgcacgg cctgccagga
     2401 atcccgatgc gcccgccgtc attgccacgc gtacggcacg cgggaacagt ttttcgtcga
     2461 caccggaaga gaggatcacc catggcatat tgatatggtc attcagacgt tgtgaagcac
     2521 agagaagctc ttgttgcgga cctttgccat aaagcggcat ttcaactttg tagaggtcag
     2581 cgccactgtc gcccagctct ttggcggcat cgatgatcgc ttgttcgcga tcgaatttat
     2641 cgccacgacg cggtgggcgg acgactggct caatgatgct taccagaccg tgtgagtggc
     2701 acagttcgtt gaactctttc accatatcca gacgttgctg cgcatcttca tcactacgcc
     2761 acagcaccag cagttttaag gctttgccac cgtcttgttt gatctgtagc ggattgattt
     2821 tgcggtcaat aaccacgcta tcgaccggaa taccgttgcc aggaatgaac tcatcggcgg
     2881 cgacaatcat ggcgcaactt ttggcaatcg cgttttgctc aaccacctgg cggtagcaga
     2941 attgttgatc taccagaatc gccgaggcat aaggcgagag ggcctttgca gcgttaactt
     3001 tgaaatcagt taaaacgcta tcggctaccg gagcgggtgc cccagccgcg gcaaacatca
     3061 tgcgcatggc ttcgcgctga tcgaccgcca gcatggcaaa accgcccgat gcgcgcgtaa
     3121 tgtcgttgat ggtgtactta ttcattcatt tattccttta tccagtcgtt acatttttac
     3181 tttggcagtc atcccggcac tgacacggac ctgttccaga atggcggacc agtcctggcg
     3241 accgcgaccc gctgcgcgcg cctggctata aacctcccgt gaggcggccc ccagcggcat
     3301 tggcacatgc agctggttgg cgacatcaag ggcgatgcca agatccttat gggcaagatc
     3361 gatcatgaag gcgggagaaa gatctccgct gaggactttg tttggccagg aagtggtgaa
     3421 gtgaccttta ccggcggcgg taccgctcat cactttgacg gcaacatcga agggaagatt
     3481 cagggcttcg cacaaaacgg cggcttctgc cgaaagcgca ttgagcgcga tgctcatgta
     3541 gttgttgatg agcttaacgc ggatccccat gcccggaccg ccagcgttga tcaactcact
     3601 gcccatcgcc atcaggatcg gtgtggcacg ttcaacttgt tcagcggtgc cgccagccag
     3661 cagtaacaga gtaccggtaa tggcatttgc agaagtacgg cctaccggaa catccatcat
     3721 gctgaagcct ttggcttgca tatcggcaat caatttatcg gtttgcagcg gatggatggt
     3781 ggacatatca atgaccagcg catcggtaga taagccttcg caaacgccgt tttcaccgaa
     3841 caacacgttg cgcaccagat cgccattcgg caacatggta atgataaatt cggcatcttt
     3901 agcggcctgc gccgggttgg cggcgggagt cgcacctttg tctaccagat gccgcacagc
     3961 ttcggcgttc acatcaaaga cgcgaagttg atgcccttgc tgcaataaat tgctcgccat
     4021 tggcgaaccc atttgtccta aaccgataaa cgcgattgct gccataaccc tctcctgaat
     4081 acagtgatgt cactttttgt catttatgac atgctttgct tgtctgtttt tgatcgtatt
     4141 tgtaatttat cgtcaaaaaa ttgacagccg tcacttttta aacaattggt gaaattaaaa
     4201 tgaacgcatc ccaaaatgtt taaggaatga ccatgattcg tgttgcttgt gtaggtataa
     4261 ccgtgatgga tcgcatctat tacgtggaag ggttaccgac ggagagcggt aaatacgtgg
     4321 cgagaaatta tacggaagtt ggtggcgggc cagcggcgac ggcagcggtt gcggcggcaa
     4381 ggctgggggc gcaggtcgat tttattggtc gcgtaggtga tgacgacacg ggcaacagcc
     4441 tgctggcaga gctggaatcc tggggcgtta acacccgtta caccaaacgg tataaccagg
     4501 cgaaatcttc gcaatccgcc atcatggtgg ataccaaagg cgagcggata atcattaact
     4561 accccagccc ggatctgctg cctgacgcag agtggttgga ggaaattgat ttctctcagt
     4621 gggatgttgt gctggcagat gtacgctggc acgacggcgc taaaaaagcc ttcaccctgg
     4681 cccgtcaggc gggtgtgatg accgttctgg acggggacat tacgccgcag gatatcagtg
     4741 agctggtggc attaagcgat cacgcggcct tttcagaacc gggtctggcg cgcttaacgg
     4801 gcgtgaaaga gatggccagt gcgctaaaac aggcacaaac gctcacaaat ggtcatgtct
     4861 atgtgaccca gggtagcgca ggctgcgact ggctggaaaa tggtgggcgt cagcatcaac
     4921 cggccttcaa agttgatgtg gtagatacca caggtgcggg tgatgttttt cacggcgctt
     4981 tggcggtggc gctggcaaca agtggggatt tagcggagtc agtccgcttc gccagcggtg
     5041 tagcggcgtt aaaatgcaca cgtcccggtg gacgagccgg gatccctgac tgtgatcaaa
     5101 cccgatcttt tttgtcactt tttgtataaa atgccagggt gatggttttt cgaggaattt
     5161 tcatgagcct taccgaacta accggtaacc cgcggcacga ccaactcctc atgctgatcg
     5221 ccgagcgtgg gtatatgaat attgatgagc tggcaaatct gctggatgtc tccacgcaga
     5281 cggtccgccg ggatattcgt aaattaagcg agcaaggcct gattacgcgc catcacggtg
     5341 gcgcgggtcg ggcttccagc gtcgttaata cggcgttcga gcagcgtgag gtttcgcaaa
     5401 ccgaggaaaa aaaagcgatt gccgaagcgg tggcagacta tattcctgat ggttcaacaa
     5461 tatttatcac cattggtacg actgttgagc atgttgcccg ggcgttactt aaccataatc
     5521 atttgcggat aatcaccaac agcctgcgtg tggcgcatat tctttaccac aacccgcgct
     5581 ttgaagtgat ggtgcccggc ggtacgttgc gctctcataa tagcgggatc attggccctt
     5641 cagcggcgtc ctttgtggct gattttcgtg ctgattatct ggtaacaagc gttggggcga
     5701 ttgagagcga tggcgcgttg atggagtttg atgtaaacga agctaacgtg gtgaaaacga
     5761 tgatggcgca cgcgagaaat attctgctgg tcgccgatca cactaagtat catgcttcgg
     5821 cggcggttga aattggtaac gtggcacagg tcactgcgct ctttaccgac gagctgccgc
     5881 ccgctgcgct aaaatcacgc ttacaagaca gccaaattga aatcattctt ccccaggaag
     5941 acgcgtagat ttttgtgacc gttaacctgg cttcatacct tgccacatag ccaaacccat
     6001 cctttcccgc tacagttaat ttcttgtggc gcgaaaggag gcaaaaatgc tctatatctt
     6061 tgatttaggt aatgtgattg tcgatatcga ctttaaccgt gtgctgggag cctggagcga
     6121 tttaacgcgt gttccactgg ctacgcttaa gaagagtttc cacatggggg aggcgtttca
     6181 tcagcatgag cgtggggaaa ttagcgacga agcgttcgca gaggcgctgt gtcatgagat
     6241 ggccctgccg ctaagctacg agcagttctc tcacggctgg caggcggtgt ttgttgcgct
     6301 gcgcccggaa gtgatcgcca tcatgcataa actgcgtgag caggggcatc gcgtggtggt
     6361 gctttccaat actaaccgcc tgcataccac cttctggccg gaagaatacc cggaaattcg
     6421 tgatgctgct gaccatatct atctgtcgca agatctgggg atgcgcaaac ctgaagcacg
     6481 aatttaccag catgttttgc aggcggaagg tttttcaccc agcgatacgg tctttttcga
     6541 cgataacgcc gataatatag aaggagccaa tcagctgggc attaccagta ttctggtgaa
     6601 agataaaacc accatcccgg actatttcgc gaaggtgtta tgctaaaaac cattcaggac
     6661 aaagccaggc accgtacccg tccactatgg gcctggctaa aactactctg gcaacgtatt
     6721 gatgaggaca acatgacaac cctggcaggt aaccttgcct atgtgtcgtt gctctcatta
     6781 gtgccgctgg ttgccgttgt ttttgcgctt ttcgccgctt ttcccatgtt ttccgacgtc
     6841 agcattcagt tacgtcactt tatttttgcc aactttctgc ctgctactgg cgatgttatt
     6901 cagcggtata tcgaacaatt tgttgccaat tccaacaaga tgaccgccgt tggggcgtgc
     6961 gggctgatcg tcaccgcgtt attgctgatg tactccatcg atagcgcgtt gaataccatc
     7021 tggcgcagta aacgagcgcg accaaaaatt tactcgttcg ccgtgtactg gatgatttta
     7081 acgctggggc cgctgctggc aggggccagt ctggcgatca gttcctattt gctctccctg
     7141 cgctgggcga gcgatctcaa tactgtcatc gataacgtgc tgcgtatttt tccgctgctg
     7201 ttgtcgtgga tctccttctg gttgctgtac agcattgttc ctaccatccg cgtacctaac
     7261 cgcgacgcga ttgtcggcgc gtttgtcgcc gcgctcctgt tcgaagcagg aaagaaaggt
     7321 ttcgcgcttt atatcaccat gttcccgtca tatcagctca tttacggcgt gttggcggtg
     7381 atccccattc tctttgtttg ggtctactgg acatggtgta tcgtcttgct tggcgcggaa
     7441 attactgtca ctctcgggga ataccgcaaa ctaaaacaag cagctgaaca agaagaagac
     7501 gacgaaccat gattgcatta attcaacgcg taacccgtgc cagcgtcacc gtggagggag
     7561 aagtgacggg cgaaattggc gcgggacttt tggtgttatt gggtgtcgaa aaggatgacg
     7621 acgaacagaa agcaaaccgt ctgtgcgagc gtgtgctcgg ctaccgcatc tttagcgatg
     7681 ccgaaggcaa gatgaatctc aacgtgcaac aggcgggcgg cagtgtactg gtggtttccc
     7741 agtttaccct cgccgcagat accgaacggg ggatgcgccc aagtttctct aaaggcgcat
     7801 caccggatcg cgcagaggcg ttatatgatt atttcgtcga acgctgccgc cagcaagaga
     7861 tgaacacgca aacaggacgc ttcgctgccg atatgcaggt atcgctggtc aatgatggcc
     7921 ccgtgacatt ctggttgcag gtatgagcca gcttccaggg ttgtcacggg aaacaagaga
     7981 gagtatcgct atgtatcacc ttcgggttcc acaaacagaa gaagagttag agcgttacta
     8041 tcagtttcgc tgggaaatgt tgcgtaagcc cctgcatcaa ccaaaaggtt cggaacgcga
     8101 cgcgtgggat gcgatggcgc atcaccagat ggtcgtcgac gagcagggta atctggtggc
     8161 ggtaggccga ttgtatatta atgccgacaa tgaagcgtcc attcgcttta tggccgttca
     8221 cccggacgtg caggacaaag ggttaggaac gctgatggcg atgaccctgg agtcggtggc
     8281 gcgtcaggaa ggcgttaagc gcgtgacctg tagcgcccgt gaagacgcgg tggagttttt
     8341 cgccaagctg gggtttgtta atcagggaga aatcaccacg ccaaccacca cgccgattcg
     8401 ccattttttg atgattaagc ccgtcgccac tctggatgat attttgcatc gcggcgactg
     8461 gtgcgcgcag ctgcaacagg cgtggtacga acatatcccg cttagtgaaa aaatgggcgt
     8521 gcgtattcaa caatataccg ggcaaaaatt tatcaccacc atgccggaaa ccggtaatca
     8581 gaatccgcac catacgctgt ttgccgggag tttattctca ctggcaacgc tcaccggttg
     8641 ggggcttatc tggctgatgc tgcgcgaacg ccatctcggc ggaacgatta ttctggcgga
     8701 tgcgcatatc cgctacagca agccgattag cggtaaacct catgcggtag ccgaccttgg
     8761 tgccttaagc ggcgatctcg accgtctggc gcgcggacga aaagcacggg tgcagatgca
     8821 agttgaaatc tttggcgacg agacgccggg tgcagtgttt gaaggcacgt atatcgttct
     8881 gcccgcgaag ccatttggcc cgtatgaaga gggcgggaac gaagaagagt aggggcgttt
     8941 tacttgactg aaaaggtttt caatctgaaa accttttcag ggcccaggtc cgaagttacc
     9001 ttcccgctct ccaggcaaca tcccgtagtg ccgatcgcgc ggcctcactg ataccaccta
     9061 actgaaaaaa tccgtggata acgccaaggt agcgttggca ggtgcattgc actccttgcc
     9121 ctgtcatgcg gtgatacaac gcttcacctt catcgcaaag tgggtcgtat tcagcggtaa
     9181 tgatatgtac aggtggcaag ccattgaaat catcgcgcca aagagggctg gcttcaggat
     9241 gcctgcgttc aatgcctggc agatacatct caaagccgct cagtaaagtg tcgcgtgtga
     9301 tgatgtaatc gtgaccattg cgtatgtagc tttcgaaatg cgcagtcgca tcaagcatag
     9361 gataaattaa gatgagttgt gccggttgcc actcgccagc acgctttaat cgcaaagcag
     9421 ttaccagcgc cagatgtccg cccgcactgt caccggcaag agttatacgt tgtttatcaa
     9481 cgccgagtcg ttcggcgtgt tgccagacta tttctgcacc tcgttgcgca tcatcatggg
     9541 cagcggggaa agtatgttcc ggtgccagcc gatactggac ggcaataacc cgacattgcc
     9601 cgtaataggc taactggcgc agctggttgt cgtgagttgc gaacccgccg ctaacaaaac
     9661 agccaccgta gtaataaatt atcgtcggga gtaatgtcgg ggcattcagc ggcgacacta
     9721 ttcgtaaggt catcccttca agttcgatag tctgaatatc tacccgcgtt tctgtctcac
     9781 ccgcaaggac agcgctggcg atatagccct ccctgcgctg cgcaatactt tgttgacgcg
     9841 atgaggggcg acccgcagcg ataaactttt cgactaactc tgcaattcct ttttccagtg
     9901 ccatagtaga acttattttg ctgtatgaat gtacagtctt atacacccgt ttttgctgga
     9961 tggatacagg gaaattcacg ggcgtgaaaa atctggaaag cgcctcgcaa atctaaagac
    10021 aaacccggca gagacgtggt tttgctggag cactatcagt aagctagtgg tttcaagggt
    10081 acgtcaatga cgtacaaaaa ggattgttac tctaagccaa tggcgtataa ttatgctctt
    10141 catcgaaacg gaaattttta ctgaagatgt tcaaaaactg ctgaccgacg atgaattcag
    10201 tcgttttcag tttttcctgg ccttgaatcc tgactatggt gaggtaatac ctgaaactgg
    10261 tgggttaaga aaggttcgat gggtgtcagg aggaaagggg aaacgtgctg gtgtcagggt
    10321 tatttacttt catcaggtta aacattatga gatcagatta ctgcttattt atcgtaaggg
    10381 tattaaagat gatctgtctc cccaggaaaa agcaatgctc cggttgttaa atacgaggtg
    10441 gtaaatggat aaggcacttt ttgagcggtt gactcacagc atggcccaga tgaatgaaat
    10501 tattgaagga acgcgacagc cttcgcggac ttttgaagtt gatgctatga aaatcaaaga
    10561 aatacgccgc gcttcagggt tgtcacaatc taaatttgcc gatcttattt ctgtaagcgt
    10621 agacacgttg cgtaactggg agcaggggcg acgttcaccg accgggcctg caaaggcttt
    10681 gctgcgagcg attgccaatg atccgcaaca tgtgctgcaa gcacttaacc gttagaggca
    10741 tggttatgcc atacagaaag cactagcatt cagaactcct gtgaaagcgg catagtggtc
    10801 gcctgcccag gagttttgct atgacccgta atcgtgctga acgtcgccac cctatgatgc
    10861 gattaaaaaa actcatcgtc gcgctgatga ttgacgtcaa tgctatcgga tgataccggg
    10921 gggttccgga aggaattcct tgtttcccga actgttacca acaaaattat aacgatgaac
    10981 aagtaaagac cggttggtgg cagtactgac cggtctgtga tggatgatta atttcacctt
    11041 aaatccgggt gctgttagga tgctgacatt tttcgggtac accattcaga ttatgcaaat
    11101 aaagatccaa tc
//
LOCUS       DAZKHF020000148        10993 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2201S31241
            SAMN43267800-rid25106413.denovo.149, whole genome shotgun sequence.
ACCESSION   DAZKHF020000148 DAZKHF020000000
VERSION     DAZKHF020000148.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267800
            Sequence Read Archive: SRR30307263
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 10993)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10993)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 10993)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350581.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350581.2
            Genome Coverage       :: 151x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:21:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,652
            CDSs (total)                      :: 5,557
            Genes (coding)                    :: 5,320
            CDSs (with protein)               :: 5,320
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 237
            CDSs (without protein)            :: 237
            Pseudo Genes (ambiguous residues) :: 0 of 237
            Pseudo Genes (frameshifted)       :: 102 of 237
            Pseudo Genes (incomplete)         :: 130 of 237
            Pseudo Genes (internal stop)      :: 52 of 237
            Pseudo Genes (multiple problems)  :: 41 of 237
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10993
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267800-rid25106413.denovo.149"
                     /serotype="O 26:H11"
                     /isolate="2201S31241"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-01"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            complement(23..1852)
                     /gene="glmS"
                     /locus_tag="ACHOSC_RS15650"
                     /old_locus_tag="pgaptmp_003130"
     CDS             complement(23..1852)
                     /gene="glmS"
                     /locus_tag="ACHOSC_RS15650"
                     /old_locus_tag="pgaptmp_003130"
                     /EC_number="2.6.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004360 - glutamine-fructose-6-phosphate
                     transaminase (isomerizing) activity [Evidence IEA];
                     GO_process: GO:1901137 - carbohydrate derivative
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--fructose-6-phosphate transaminase
                     (isomerizing)"
                     /protein_id="HGU4549027.1"
                     /translation="MCGIVGAIAQRDVAEILLEGLRRLEYRGYDSAGLAVVDAEGHMT
                     RLRRLGKVQMLAQAAEEHPLHGGTGIAHTRWATHGEPSEVNAHPHVSEHIVVVHNGII
                     ENHEPLREELKARGYTFVSETDTEVIAHLVNWELKQGGTLREAVLRAIPQLRGAYGTV
                     IMDSRHPDTLLAARSGSPLVIGLGMGENFIASDQLALLPVTRRFIFLEEGDIAEITRR
                     SVNIFDKTGAEVKRQDIESNLQYDAGDKGIYRHYMQKEIYEQPNAIKNTLTGRISHGQ
                     VDLSELGPNADELLSKVEHIQILACGTSYNSGMVSRYWFESLAGIPCDVEIASEFRYR
                     KSAVRRNSLMITLSQSGETADTLAGLRLSKELGYLGSLAICNVPGSSLVRESDLALMT
                     NAGTEIGVASTKAFTTQLTVLLMLVAKLSRLKGLDASIEHDIVHGLQALPSRIEQMLS
                     QDKRIEALAEDFSDKHHALFLGRGDQYPIALEGALKLKEISYIHAEAYAAGELKHGPL
                     ALIDADMPVIVVAPNNELLEKLKSNIEEVRARGGQLYVFADQDAGFVSSDNMHIIEMP
                     HVEEVIAPIFYTVPLQLLAYHVALIKGTDVDQPRNLAKSVTVE"
     gene            complement(2014..3384)
                     /gene="glmU"
                     /locus_tag="ACHOSC_RS15655"
                     /old_locus_tag="pgaptmp_003131"
     CDS             complement(2014..3384)
                     /gene="glmU"
                     /locus_tag="ACHOSC_RS15655"
                     /old_locus_tag="pgaptmp_003131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0003977 - UDP-N-acetylglucosamine
                     diphosphorylase activity [Evidence IEA];
                     GO_function: GO:0019134 - glucosamine-1-phosphate
                     N-acetyltransferase activity [Evidence IEA];
                     GO_process: GO:0000902 - cell morphogenesis [Evidence
                     IEA];
                     GO_process: GO:0006048 - UDP-N-acetylglucosamine
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-N-acetylglucosamine
                     diphosphorylase/glucosamine-1-phosphate
                     N-acetyltransferase GlmU"
                     /protein_id="HGU4549028.1"
                     /translation="MLNNAMSVVILAAGKGTRMYSDLPKVLHTLAGKAMVQHVIDAAN
                     ELGAAHVHLVYGHGGDLLKQALKDDNLNWVLQAEQLGTGHAMQQAAPFFADDEDILML
                     YGDVPLISVETLQRLRDAKPQGGIGLLTVKLDDPTGYGRITRENGKVTGIVEHKDATD
                     EQRQIQEINTGILIANGADMKRWLAKLTNNNAQGEYYITDIIALAYQEGREIVAVHPQ
                     RLSEVEGVNNRLQLSRLERVYQSEQAEKLLLAGVMLRDPARFDLRGTLTHGRDVEIDT
                     NVIIEGNVTLGHRVKIGTGCVIKNSVIGDDCEISPYTVVEDANLAAACTIGPFARLRP
                     GAELLEGAHVGNFVEMKKARLGKGSKAGHLTYLGDAEIGDNVNIGAGTITCNYDGANK
                     FKTIIGDDVFVGSDTQLVAPVTVGKGATIAAGTTVTRNVGENALAISRVPQTQKEGWR
                     RPVKKK"
     gene            complement(3735..4154)
                     /gene="atpC"
                     /locus_tag="ACHOSC_RS15660"
                     /old_locus_tag="pgaptmp_003132"
     CDS             complement(3735..4154)
                     /gene="atpC"
                     /locus_tag="ACHOSC_RS15660"
                     /old_locus_tag="pgaptmp_003132"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001251972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit epsilon"
                     /protein_id="HGU4549029.1"
                     /translation="MAMTYHLDVVSAEQQMFSGLVEKIQVTGSEGELGIYPGHAPLLT
                     AIKPGMIRIVKQHGHEEFIYLSGGILEVQPGNVTVLADTAIRGQDLDEARAMEAKRKA
                     EEHISSSHGDVDYAQASAELAKAIAQLRVIELTKKAM"
     gene            complement(4175..5557)
                     /gene="atpD"
                     /locus_tag="ACHOSC_RS15665"
                     /old_locus_tag="pgaptmp_003133"
     CDS             complement(4175..5557)
                     /gene="atpD"
                     /locus_tag="ACHOSC_RS15665"
                     /old_locus_tag="pgaptmp_003133"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017800291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit beta"
                     /protein_id="HGU4549030.1"
                     /translation="MATGKIVQVIGAVVDVEFPQDAVPRVYDALEVQNGNERLVLEVQ
                     QQLGGGIVRTIAMGSSDGLRRGLDVKDLEHPIEVPVGKATLGRIMNVLGEPVDMKGEI
                     GEEERWAIHRAAPSYEELSNSQELLETGIKVIDLMCPFAKGGKVGLFGGAGVGKTVNM
                     MELIRNIAIEHSGYSVFAGVGERTREGNDFYHEMTDSNVIDKVSLVYGQMNEPPGNRL
                     RVALTGLTMAEKFRDEGRDVLLFVDNIYRYTLAGTEVSALLGRMPSAVGYQPTLAEEM
                     GVLQERITSTKTGSITSVQAVYVPADDLTDPSPATTFAHLDATVVLSRQIASLGIYPA
                     VDPLDSTSRQLDPLVVGQEHYDTARGVQSILQRYQELKDIIAILGMDELSEEDKLVVA
                     RARKIQRFLSQPFFVAEVFTGSPGKYVSLKDTIRGFKGIMEGEYDHLPEQAFYMVGSI
                     EEAVEKAKKL"
     gene            complement(5584..6447)
                     /gene="atpG"
                     /locus_tag="ACHOSC_RS15670"
                     /old_locus_tag="pgaptmp_003134"
     CDS             complement(5584..6447)
                     /gene="atpG"
                     /locus_tag="ACHOSC_RS15670"
                     /old_locus_tag="pgaptmp_003134"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020882764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit gamma"
                     /protein_id="HGU4549031.1"
                     /translation="MAGAKEIRSKIASVQNTQKITKAMEMVAASKMRKSQDRMAASRP
                     YADTMRKVIGHLASGNLEYKHPYLEERDVKRVGYLVVSTDRGLCGGLNINLFKKLLAE
                     MKTWTDKGVQCDLAMIGSKGVSFFNSVGGNVVAQVTGMGDNPSLSELIGPVKVMLQAY
                     DEGRLDKLYIVSNKFINTMSQVPTISQLLPLPASDDDDLKHKSWDYLYEPDPKALLDT
                     LLRRYVESQVYQGVVENLASEQAARMVAMKAATDNGGSLIKELQLVYNKARQASITQE
                     LTEIVSGAAAV"
     gene            complement(6498..8039)
                     /gene="atpA"
                     /locus_tag="ACHOSC_RS15675"
                     /old_locus_tag="pgaptmp_003135"
     CDS             complement(6498..8039)
                     /gene="atpA"
                     /locus_tag="ACHOSC_RS15675"
                     /old_locus_tag="pgaptmp_003135"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001176751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit alpha"
                     /protein_id="HGU4549032.1"
                     /translation="MQLNSTEISELIKQRIAQFNVVSEAHNEGTIVSVSDGVIRIHGL
                     ADCMQGEMISLPGNRYAIALNLERDSVGAVVMGPYADLAEGMKVKCTGRILEVPVGRG
                     LLGRVVNTLGAPIDGKGPLDHDGFSAVEAIAPGVIERQSVDQPVQTGYKAVDSMIPIG
                     RGQRELIIGDRQTGKTALAIDAIINQRDSGIKCIYVAIGQKASTISNVVRKLEEHGAL
                     ANTIVVVATASESAALQYLAPYAGCAMGEYFRDRGEDALIIYDDLSKQAVAYRQISLL
                     LRRPPGREAFPGDVFYLHSRLLERAARVNAEYVEAFTKGEVKGKTGSLTALPIIETQA
                     GDVSAFVPTNVISITDGQIFLETNLFNAGIRPAVNPGISVSRVGGAAQTKIMKKLSGG
                     IRTALAQYRELAAFSQFASDLDDATRKQLDHGQKVTELLKQKQYAPMSVAQQSLVLFA
                     AERGYLADVELSKIGSFEAALLAYVDRDHAPLMQEINQTGGYNDEIEGKLKGILDSFK
                     ATQSW"
     gene            complement(8052..8585)
                     /gene="atpH"
                     /locus_tag="ACHOSC_RS15680"
                     /old_locus_tag="pgaptmp_003136"
     CDS             complement(8052..8585)
                     /gene="atpH"
                     /locus_tag="ACHOSC_RS15680"
                     /old_locus_tag="pgaptmp_003136"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015966593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit delta"
                     /protein_id="HGU4549033.1"
                     /translation="MSEFITVARPYAKAAFDFAVEHQSVERWQDMLAFAAEVTKNEQM
                     AELLSGALAPETLAESFIAVCGEQLDENGQNLIRVMAENGRLNALPDVLEQFIHLRAV
                     SEATAEVDVISAAALSEQQLAKISAAMEKRLSRKVKLNCKIDKSVMAGVIIRAGDMVI
                     DGSVRGRLERLADVLQS"
     gene            complement(8600..9070)
                     /gene="atpF"
                     /locus_tag="ACHOSC_RS15685"
                     /old_locus_tag="pgaptmp_003137"
     CDS             complement(8600..9070)
                     /gene="atpF"
                     /locus_tag="ACHOSC_RS15685"
                     /old_locus_tag="pgaptmp_003137"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001052215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015078 - proton transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit B"
                     /protein_id="HGU4549034.1"
                     /translation="MNLNATILGQAIAFVLFVLFCMKYVWPPLMAAIEKRQKEIADGL
                     ASAERAHKDLDLAKASATDQLKKAKAEAQVIIEQANKRRSQILDEAKAEAEQERTKIV
                     AQAQAEIEAERKRAREELRKQVAILAVAGAEKIIERSVDEAANSDIVDKLVAEL"
     gene            complement(9132..9371)
                     /gene="atpE"
                     /locus_tag="ACHOSC_RS15690"
                     /old_locus_tag="pgaptmp_003138"
     CDS             complement(9132..9371)
                     /gene="atpE"
                     /locus_tag="ACHOSC_RS15690"
                     /old_locus_tag="pgaptmp_003138"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004246596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015078 - proton transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit C"
                     /protein_id="HGU4549035.1"
                     /translation="MENLNMDLLYMAAAVMMGLAAIGAAIGIGILGGKFLEGAARQPD
                     LIPLLRTQFFIVMGLVDAIPMIAVGLGLYVMFAVA"
     gene            complement(9418..10233)
                     /gene="atpB"
                     /locus_tag="ACHOSC_RS15695"
                     /old_locus_tag="pgaptmp_003139"
     CDS             complement(9418..10233)
                     /gene="atpB"
                     /locus_tag="ACHOSC_RS15695"
                     /old_locus_tag="pgaptmp_003139"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045263 - proton-transporting ATP
                     synthase complex, coupling factor F(o) [Evidence IEA];
                     GO_function: GO:0015078 - proton transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit A"
                     /protein_id="HGU4549036.1"
                     /translation="MASENMTPQDYIGHHLNNLQLDLRTFSLVDPQNPPATFWTINID
                     SMFFSVVLGLLFLVLFRSVAKKATSGVPGKFQTAIELVIGFVNGSVKDMYHGKSKLIA
                     PLALTIFVWVFLMNLMDLLPIDLLPYIAEHVLGLPALRVVPSADVNVTLSMALGVFIL
                     ILFYSIKMKGIGGFTKELTLQPFNHWAFIPVNLILEGVSLLSKPVSLGLRLFGNMYAG
                     ELIFILIAGLLPWWSQWILNVPWAIFHILIITLQAFIFMVLTIVYLSMASEEH"
     gene            complement(10242..10622)
                     /gene="atpI"
                     /locus_tag="ACHOSC_RS15700"
                     /old_locus_tag="pgaptmp_003140"
     CDS             complement(10242..10622)
                     /gene="atpI"
                     /locus_tag="ACHOSC_RS15700"
                     /old_locus_tag="pgaptmp_003140"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418195.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit I"
                     /protein_id="HGU4549037.1"
                     /translation="MSVSLVSRNVARKLLLVQLLVVIASGLLFSLKDPFWGVSAISGG
                     LAVFLPNVLFMIFAWRHQAHTPAKGRVAWTFAFGEAFKVLAMLVLLVVALAVLKAVFL
                     PLIVTWVLVLVVQILAPAVINNKG"
BASE COUNT         2621 a         3006 c         2720 g         2646 t
ORIGIN      
        1 ccttttttat ctatgatagt gattactcaa ccgtaaccga ttttgccagg ttacgcggct
       61 ggtcaacgtc ggtgcctttg atcagcgcga catggtaagc cagcagctgc agcggaacgg
      121 tgtagaagat cggtgcaatc acctcttcca catgcggcat ctcgatgatg tgcatgttat
      181 cgctacttac aaaacccgca tcctgatcgg cgaagacata caactgaccg ccacgcgcgc
      241 gaacttcttc aatgttggat ttcagttttt ccaacagttc gttgttcggt gcaacgacga
      301 taaccggcat atccgcgtca atcagcgcca gcgggccgtg tttcagctcg cctgcagcgt
      361 aggcttcagc gtgaatgtaa gagatctctt tcagcttcaa tgcgccttcc agcgcgattg
      421 ggtactgatc gccacggccc aggaacagcg cgtgatgttt gtcagagaaa tcttctgcca
      481 gagcttcaat gcgtttgtcc tgagacagca tctgctcaat acggctcggc agcgcctgca
      541 gaccatgcac aatgtcatgt tcaatggagg catccagacc tttcaggcga gacagcttcg
      601 ccaccagcat caacagcaca gttaactgag tggtgaatgc tttagtggat gccacgccga
      661 tttccgtacc cgcgttggtc atcagcgcca gatcggattc gcgcaccaga gaagaacccg
      721 gaacgttaca gattgccagc gaaccaaggt aacccagctc tttcgacaga cgcaggcccg
      781 ccagggtatc cgcggtttcg ccagactgtg acaaggtgat catcaggctg ttacgacgca
      841 cggcagattt gcgatagcgg aattcagagg cgatttcgac gtcgcacgga atacctgcca
      901 gcgattcaaa ccagtaacgg gaaaccatac cggagttata agaagtacca caggcgagga
      961 tctgaatatg ctcaaccttc gacagcagtt cgtcggcgtt cggtcccagc tcgcttaaat
     1021 caacctgacc gtggctgatg cgcccggtaa gggtgttttt gatcgcgttc ggctgttcgt
     1081 agatctcttt ctgcatgtag tgacggtaaa tacctttatc gcccgcgtca tattgcagat
     1141 tggattcgat atcctgacgt tttacttccg cgccagtttt atcgaagatg tttaccgaac
     1201 ggcgagtaat ttccgcaata tcgccctctt caaggaagat aaagcgacgg gtcaccggca
     1261 gcagcgccag ctggtcagaa gcgataaagt tttcgcccat acccagacca atcaccagcg
     1321 gactaccaga acgtgccgcc agcagggtat ccgggtgacg ggagtccatg atcactgtac
     1381 cgtacgcacc acgcagttgc gggatagcac gcagaacagc ctcacgcaga gtaccgccct
     1441 gtttcagctc ccagttcacc agatgggcaa ttacttcggt gtctgtttca gaaacgaagg
     1501 tatagccacg cgcttttaac tcttcgcgca gcggttcatg gttttcgatg atgccgttat
     1561 gcaccaccac aatgtgttca gaaacatgcg gatgcgcatt cacttctgaa ggttcaccgt
     1621 gggtcgccca gcgagtatga gcaatacccg taccgccatg cagaggatgt tcttccgctg
     1681 cctgagccag catctggact ttaccgaggc gacgcaggcg ggtcatatga ccttccgcat
     1741 caacaacggc cagaccggca gagtcatatc cgcggtattc cagacgacgt aaaccttcaa
     1801 gaaggatttc tgctacatca cgttgcgcga tcgcgccaac aattccacac atagtttttg
     1861 attccgattt atatcgttgt cggtcaacct gtatgcccgt tttttcgggc gccccgagcc
     1921 ttgtagagag tggggttatt tttatagtta ctgcttgtgg gagggagatt atgttatctc
     1981 ctcatcccat gtgaccggat tacccggcca gaatcacttt ttctttaccg gacgacgcca
     2041 gccttctttc tgagtctgcg gcacacggct gatagctaat gcattttcgc cgacattacg
     2101 cgtcacagtt gtacccgcag caatggtcgc gcctttgcct actgttaccg gggccaccag
     2161 ctgagtgtcg gaaccgacaa acacatcgtc gccgataatg gtcttaaatt tattcgcacc
     2221 atcgtagttg caggtaatgg ttcccgcgcc gatgttaacg ttatcgccaa tttccgcatc
     2281 gcccaggtaa gtcagatgac cagctttcga gcctttaccc agacgcgctt ttttcatctc
     2341 aacaaagtta ccgacgtgtg caccttccag caactcagca ccaggacgca gtcgggcaaa
     2401 cgggccaata gtacaggccg ctgccagatt cgcgtcttcc acaacggtat acggactgat
     2461 ttcgcaatca tcaccaatca cgctgttttt aatcacgcaa ccggtgccga ttttcacgcg
     2521 atgaccgaga gtcacgttgc cctcgatgat aacgttagta tcaatttcaa catcgcgccc
     2581 gtgagtaagc gtaccgcgca aatcaaaacg cgccggatcg cgcagcataa cgcctgctaa
     2641 cagcagtttt tcagcctgtt cggactggta aacgcgctcc agacgggaga gttgcaggcg
     2701 gttattcaca ccttctactt cgcttaaacg ttgcggatga acggcgacga tttcacgccc
     2761 ttcctgatac gccagcgcaa taatgtcggt gatgtagtat tcgccctgag cattattgtt
     2821 ggtcagcttc gccagccagc gtttcatatc tgcgccgtta gcgatcagaa tgccggtgtt
     2881 gatctcctga atctgacgct gctcgtcggt ggcatctttg tgctcaacaa tgccggtaac
     2941 tttgccattt tcacgggtga tacgtccata accggtcgga tcatccagtt tcaccgtcag
     3001 cagaccaatg ccaccctgcg gtttagcatc acgcaggcgc tggagtgttt cgacagagat
     3061 cagcggcacg tcgccgtaga gcattaaaat gtcttcatca tcggcaaaga aaggtgcggc
     3121 ctgctgcatt gcatgacccg tacccagctg ctctgcctga agcacccagt taaggttgtc
     3181 gtctttcagc gcctgtttaa gcagatcgcc gccgtgaccg tacaccaggt gaacgtgcgc
     3241 tgcgcctaat tcattcgcag catcaatgac atgctgaacc atcgctttcc cggcaagggt
     3301 atgcagcact ttcggaagat cggaatacat gcgcgtgcct ttgcctgcgg caaggatcac
     3361 tacgctcata gcattattca acatacgcgt cctgactgta atttgagaac gaatttaaac
     3421 cgcttcacct tgaaaaaact acattttttt catcgtgaaa tggacagagg ataaattgtt
     3481 caataacgga ttatccccgc tgacgaaagt gccattttcg accatcgtca cgccttttgt
     3541 ctgcagaaaa ttaaggcagt accttcattt aagcgtcagg tggatgtttt tgctcttatt
     3601 tcgatcaatg aataaacggg aaaacagggg tgttttatat cagcaggatc tatgtgaacg
     3661 ctcttcagga cggatcacgc gtaaaaaaaa gccagcctgt ttccagactg gcttttgtgc
     3721 ttttcaagcc ggtgttacat cgcttttttg gtcaactcga taacgcgcag ctgcgcgatc
     3781 gctttggcca gttccgcaga cgcctgagcg taatctacgt cgccgtgaga gctgctaatg
     3841 tgctcttcag ccttacgttt cgcttccatg gctcgcgctt cgtcgagatc ctggccgcga
     3901 attgcggtgt cggccagaac ggtcacgttg ccaggctgca cttcaagaat gccgccagac
     3961 agatagataa actcttcgtg accgtgctgt ttcacgatgc gaatcatacc aggcttaatg
     4021 gcggtgagca gcggtgcgtg gccagggtag atccccagtt caccttcgct acccgttacc
     4081 tggattttct cgaccagacc agagaacatt tgttgctctg cgctgacgac gtccaggtgg
     4141 taagtcattg ccatatcacc ctccgattaa ggcgttaaag ttttttggct ttttccacag
     4201 cttcttcgat ggaaccgacc atgtagaacg cctgctccgg caggtgatcg tattcgcctt
     4261 ccatgatgcc tttaaagcca cggatggtgt ctttcaggga gacgtattta cccggagaac
     4321 cggtgaatac ttctgccacg aagaacggct gggacaggaa gcgctggatc ttacgagcac
     4381 gcgctaccac cagtttgtct tcttcagaca gttcatccat acccaggatg gcgatgatgt
     4441 ctttcagttc ctgataacgt tgcaggatgg actgaacgcc acgcgcggtg tcgtagtgtt
     4501 cctgaccaac caccagcggg tccagctgac ggctggtgga gtccagcggg tcaacggccg
     4561 ggtagatacc cagagacgcg atctgacggc tcagtaccac ggttgcgtca aggtgcgcaa
     4621 aggtggttgc cggagacgga tcagtcaagt catccgcagg tacgtatacc gcctgtacgg
     4681 aggtgataga accagttttg gtggaggtga tacgttcctg cagaacgccc atctcttccg
     4741 ccagggtcgg ctgataacct accgctgaag gcatacggcc cagcagtgcg gatacttccg
     4801 taccggccag ggtgtaacga tagatgttgt caacgaacag cagaacgtca cgaccttcgt
     4861 cacggaattt ctcagccatg gtcagaccgg tcagagcaac gcgcagacgg tttcccggcg
     4921 gctcgttcat ctggccatac accagggata ctttgtcgat aacgttggag tcggtcattt
     4981 cgtggtagaa gtcgttaccc tcacgagtac gttcacctac gcccgcaaac acagagtaac
     5041 cggagtgctc gatcgcgatg ttacgaatga gctccatcat gtttacggtt ttacctacac
     5101 ccgcaccacc gaacagacca actttaccgc ccttagcgaa cggacacatc aggtcgataa
     5161 ctttgatacc ggtttccagc agttcctgag agtttgacag ctcttcgtag gaaggtgctg
     5221 cgcggtgaat cgcccaacgc tcttcttcac cgatctcgcc tttcatgtcg accggttcac
     5281 ccagtacgtt catgatacgg cccagagtcg ctttacctac cgggacttcg atcgggtgtt
     5341 cgaggtcttt tacatccaga ccgcgacgca gaccgtcgga ggaacccatt gcgatggtac
     5401 gcacgatacc gccgccgagc tgctgctgaa cttccagcac cagacgctca ttaccatttt
     5461 gcacctcaag agcatcgtac acgcgcggta cggcatcctg agggaattcg acgtcaacta
     5521 cggcgccgat tacctggaca atctttccag tagccatctt aaatcctcta cgaaataacc
     5581 tgtttaaacc gcggcggccc ccgagacgat ctcggtgagt tcctgagtaa tgctggcctg
     5641 acgagctttg ttgtatacca actgcagctc tttaatcagg ctgccgccat tgtcggtcgc
     5701 ggctttcatc gccaccatac gggcggcctg ctcgctggcc aggttttcaa ccacgccctg
     5761 ataaacctga gattcgacat aacgacgcag cagggtatcc agcaacgcct tcggatcggg
     5821 ttcgtacagg taatcccagg atttatgttt cagatcatca tcatctgatg ccggtaacgg
     5881 cagcagctgg ctgatggtcg gaacctgaga catggtgtta ataaatttgt tgctgacaat
     5941 gtaaagcttg tccagacggc cttcgtcgta ggcctgcaac atcactttta ccggaccgat
     6001 cagttcggac agggaagggt tatcccccat gccggtgacc tgggcaacaa cattgccgcc
     6061 cacggagttg aagaacgaca cgcctttcga gccgatcatt gcgaggtcgc attgaacgcc
     6121 tttgtcggtc caggtcttca tttccgccag cagttttttg aacaggttaa tgttcaaacc
     6181 accgcacaaa ccacggtcgg tcgacaccac caggtagccc acgcgtttaa cgtcgcgttc
     6241 ttccaggtat gggtgcttat attccagatt accgctcgca aggtgaccaa tcactttgcg
     6301 catggtatct gcataaggac ggctggccgc catgcgatcc tgcgatttac gcattttgga
     6361 agcggcgacc atctccatcg ctttagtgat cttttgcgtg ttctggacgc ttgcgatctt
     6421 actacgtatc tcttttgcgc cggccatgag cttctcctca atgccttgcg gcctgcccta
     6481 aggcaagccg ccagacgtta ccaggattgg gttgctttga aggaatcgag gatgcctttc
     6541 agcttgcctt cgatttcgtc gttgtagcca ccggtctggt tgatctcttg catcaacgga
     6601 gcgtgatcac ggtcgacgta agccagcaga gcggcttcga agctgccgat tttcgacagt
     6661 tcaacatccg ccaggtaacc acgttctgct gcgaacagaa ccagagactg ttgcgctacg
     6721 gacatcggcg catactgttt ctgtttcagc agttcggtca ctttctgacc gtggtcaagc
     6781 tgcttacgtg ttgcatcgtc aaggtcggat gcaaactgag agaacgctgc cagttcacga
     6841 tactgtgcca gagcggtacg gataccaccg gacagttttt tcatgatctt ggtctgtgct
     6901 gcaccaccaa cacgggatac ggaaataccc gggttaaccg caggacgaat accggcgttg
     6961 aacaggttgg tttccaggaa gatctgacca tcggtaatgg agattacgtt ggtcggaacg
     7021 aacgcagaaa cgtcacccgc ctgagtttca ataatcggca gtgcggtcag agaaccggtt
     7081 ttccctttca cttcaccttt ggtgaaggct tcaacgtatt cggcgttaac acgcgcagca
     7141 cgctccagca gacgagagtg gaggtagaat acgtcgcccg ggaatgcttc acgtcctggc
     7201 ggacgacgga gcagcaggga gatctgacgg taagcaacag cctgtttaga caggtcatcg
     7261 taaatgatca gcgcatcttc accgcggtca cggaagtatt cgcccattgc gcaaccggca
     7321 tacggagcca gatattgcag tgcagcggat tcagacgcgg ttgctaccac aacgatggtg
     7381 ttagccagtg cgccgtgctc ttccagttta cgtaccacgt tagaaatggt ggacgctttc
     7441 tggccgatag cgacatagat acatttgata ccggaatcgc gctggttgat gatggcatcg
     7501 atagccagtg cggttttacc ggtctgacgg tcaccgatga tcaattcacg ctgaccacga
     7561 ccaattggga tcatggagtc aacggcttta taaccggtct gtaccggctg atctacggac
     7621 tgacgttcga taacgcccgg agcgattgct tctacagcag agaagccgtc gtgatccagc
     7681 ggacctttac cgtcgattgg tgcacccaga gtgttaacca cacggcccag caggccacgg
     7741 ccaaccggaa cttccaggat acggccagta cacttaactt tcatgccttc ggcaaggtca
     7801 gcgtacggac ccataacaac cgcacctaca gagtcgcgct cgaggttcag tgcgatagcg
     7861 taacggttac ccggcaggga gatcatttca ccctgcatac aatcggccag gccgtgaatg
     7921 cggataacac cgtcacttac agaaacaata gtaccttcgt tgtgagcttc actcacaaca
     7981 ttgaactgag caatgcgctg cttgatcagt tcgctgattt cggtggaatt cagttgcatg
     8041 ctccagtccc cttaagactg caagacgtct gcaaggcgct caagacgacc gcgtacgctg
     8101 ccatcaatga ccatatcacc cgctcggatg ataacgcctg ccattacaga cttatcgatt
     8161 ttgcaattca gcttaacttt gcgtgacaga cgtttttcca tcgcagcaga aattttcgcg
     8221 agctgttgtt cactcagtgc ggcagcggaa atgacgtcta cctcagcggt agcctcactc
     8281 acggcacgaa ggtgaataaa ctgctccaga acatccggga gcgcgttaag acgaccattt
     8341 tcagccatta cccgaatcag gttctgaccg ttttcgtcca gttgctcacc acaaactgcg
     8401 ataaacgact cggcgagcgt ttctggcgca agcgcgccag agagaagctc tgccatttgt
     8461 tcgtttttgg ttacctcggc ggcaaacgcc agcatgtcct gccagcgttc tacactttgg
     8521 tgttcgacgg caaagtcaaa agctgctttg gcgtaggggc gagctaccgt aataaattca
     8581 gacatcagcc cctccctcct tacagttcag cgacaagttt atccacgatg tcgctgttag
     8641 cagcttcatc cacggaacgt tcgatgatct tctcggcgcc agcaacagcc aggatagcaa
     8701 cttgcttacg cagctcttca cgggcacgtt tacgctcggc ttcaatttct gcctgcgcct
     8761 gggccacgat tttagtacgt tcctgttctg cctcagcttt cgcttcgtcc agaatctgcg
     8821 agcggcgttt gttcgcctgc tcgatgatta cctgggcttc cgctttcgct tttttcagct
     8881 ggtcggtcgc gctggccttt gcaaggtcaa ggtccttatg tgctcgttct gcggaagcaa
     8941 ggccgtcagc aatttctttt tgacgttttt cgatggctgc cattaatggc ggccatacgt
     9001 acttcatgca gaacagaacg aacaggacaa acgcgatggc ctggccgagg attgttgcgt
     9061 taagattcac agcacaatgc ctctatttag ttaacgttct gatattgctc tttaaataaa
     9121 agcaacgctt actacgcgac agcgaacatc acgtacagac ccagacctac agcgatcatc
     9181 gggatagcat ccaccagacc cataacgata aagaactgag tacgcagcag aggaatcaga
     9241 tcaggttgac gcgctgcgcc ttccaggaat ttacccccga ggatgccgat accgatcgca
     9301 gcaccgattg ccgccagacc catcatcaca gcggcagcca tgtacagcag atccatattc
     9361 aggttttcca tgacagtctc cagtttgttt cagttaaaac gtagtagtgt tggtaaatta
     9421 atgttcttca gacgccatcg acagatagac gatcgtcaga accatgaaga tgaaggcttg
     9481 cagcgtaatg atcaggatgt ggaaaatggc ccacggcaca ttcaggatcc actgtgacca
     9541 ccacggcaac agaccagcaa tcagaatgaa aatcagctca ccggcataca tgttaccgaa
     9601 cagtcgcaaa ccaagtgaaa ctggtttgga cagcaggctt accccttcaa ggattaagtt
     9661 gacaggaatg aacgcccagt gattgaacgg ctgcagcgtc aactctttcg tgaagccgcc
     9721 gatgcctttc attttgatgc tgtagaacag aatcaggata aatacgccca atgccataga
     9781 cagcgttacg ttcacgtccg cagacggaac cacacgcagt gcaggcagac ccagtacatg
     9841 ttcagcaatg tacggcagca ggtcgatagg cagtaaatcc atcaggttca tcaggaatac
     9901 ccagacgaag atcgtcaggg ccagcggagc aatcagcttg cttttgccat ggtacatgtc
     9961 tttcacgcta ccattaacaa agccgatcac cagctcaatc gcggtctgaa acttacctgg
    10021 cacaccgctg gtcgcctttt tggctacgct acggaataaa accaggaaca acagacccag
    10081 caccaccgag aagaacatgg agtcaatatt gattgtccag aaggtggctg gggggttttg
    10141 tggatccacc agcgagaatg tacgcaggtc cagctgaagg ttattcaggt ggtgtcctat
    10201 gtaatcctgc ggcgtcatat tttctgaagc catgatgcct tttacccttt gttgttaatt
    10261 acagccggtg ccagtatctg aaccaccagc accaaaaccc acgtaacgat cagcggcaag
    10321 aataccgcct ttaaaaccgc caacgccacc accagtaaca ccaacatcgc cagaactttg
    10381 aaagcttcgc caaatgcgaa tgtccaggcc acccggcctt tcgctggtgt atgcgcctgg
    10441 tgacgccagg caaatatcat aaacaaaacg ttaggcagaa agactgccag gcccccgctt
    10501 attgcagaga cgccccagaa ggggtctttg aggctgaaca gcaatccact tgctatcacc
    10561 accagtaact gaacgagcag aagcttccga gcaacgtttc gactcacgag cgacacagac
    10621 atcacgtttt tcactcctgc tcccttcgag gtatgccgcg tgtcgtataa aactttcttt
    10681 aaggcttaga gtcaagcatc aaaaagcggt caaattatac ggtgcgcccc cgtgatttca
    10741 aacaataagt agccaaaagg tgaataaatg tttaaatatt tttccagtgc atacaattgc
    10801 gacttttctg ctaaccctgt tcgatcatga aaaactgtaa ataacgcgta aacactggtg
    10861 ataaagcgtg cttcagatca catattgcgc atgttcgcgc acagcatatt tatttacttg
    10921 gcaaatgatg cctttgcaag tttatgatat ttcagtctaa aaacagatac tgttttaata
    10981 aatgacattt aca
//
LOCUS       DAZKHF020000149        10935 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2201S31241
            SAMN43267800-rid25106413.denovo.150, whole genome shotgun sequence.
ACCESSION   DAZKHF020000149 DAZKHF020000000
VERSION     DAZKHF020000149.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267800
            Sequence Read Archive: SRR30307263
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 10935)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10935)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 10935)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350581.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350581.2
            Genome Coverage       :: 151x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:21:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,652
            CDSs (total)                      :: 5,557
            Genes (coding)                    :: 5,320
            CDSs (with protein)               :: 5,320
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 237
            CDSs (without protein)            :: 237
            Pseudo Genes (ambiguous residues) :: 0 of 237
            Pseudo Genes (frameshifted)       :: 102 of 237
            Pseudo Genes (incomplete)         :: 130 of 237
            Pseudo Genes (internal stop)      :: 52 of 237
            Pseudo Genes (multiple problems)  :: 41 of 237
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10935
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267800-rid25106413.denovo.150"
                     /serotype="O 26:H11"
                     /isolate="2201S31241"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-01"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            complement(11..607)
                     /gene="nrfG"
                     /locus_tag="ACHOSC_RS15705"
                     /old_locus_tag="pgaptmp_003141"
     CDS             complement(11..607)
                     /gene="nrfG"
                     /locus_tag="ACHOSC_RS15705"
                     /old_locus_tag="pgaptmp_003141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme lyase NrfEFG subunit NrfG"
                     /protein_id="HGU4549038.1"
                     /translation="MKEPKIPVKMLTTLTILMVFLCVGSYLLSPKWQAVHAEYQRQRD
                     PLHQFASQQTPEAQLQALQDKIRANPQNSEQWALLGEYYLWQNDYSNSLLAYRQALQL
                     RGENAELYAALATVLYYQASQHMTAQTRAMIDKALALDSNEITALMLLASDAFMQANY
                     AQAIELWQKVMDLNSPRINRTQLVESINMAKLLQRRSD"
     gene            complement(604..987)
                     /gene="nrfF"
                     /locus_tag="ACHOSC_RS15710"
                     /old_locus_tag="pgaptmp_003142"
     CDS             complement(604..987)
                     /gene="nrfF"
                     /locus_tag="ACHOSC_RS15710"
                     /old_locus_tag="pgaptmp_003142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme lyase NrfEFG subunit NrfF"
                     /protein_id="HGU4549039.1"
                     /translation="MNKGLLTLLLLFTCFARAQVVDTWQFANPQQQQQALNIASQLRC
                     PQCQNQNLLESNAPVAVSMRHQVYSMVAEGKNEVEIIGWMTERYGDFVRYNPPLTGQT
                     LVLWALPVVLLRLMALILWRVRAKR"
     gene            complement(980..2698)
                     /gene="nrfE"
                     /locus_tag="ACHOSC_RS15715"
                     /old_locus_tag="pgaptmp_003143"
     CDS             complement(980..2698)
                     /gene="nrfE"
                     /locus_tag="ACHOSC_RS15715"
                     /old_locus_tag="pgaptmp_003143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014640253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme lyase CcmF/NrfE family subunit"
                     /protein_id="HGU4549040.1"
                     /translation="MDVFLPEVGFLALLLSLGVNVLTPLTAFAGVRLRWPAMMRLTCI
                     GILAQFALLLLAFGVLTYCFLISDFSVIYVAQHSYSLLSWELKLAAVWGGHEGSLLLW
                     VLLLSAWSALFAWHYRQQTDPLFPLTLAVLSLMLAALLLFVVLWSDPFVRIFPPAIEG
                     RDLNPMLQHPGLIFHPPLLYLGYGGLMVAASVALASLLRGEFDGACARICWRWALPGW
                     SALTAGIILGSWWAYCELGWGGWWFWDPVENASLLPWLSATALLHSLSLTRQREIFRH
                     WSLLLAIVTLMLSLLGTLIVRSGILVSVHAFALDNVRAVPLFSLFALISLASLALYGW
                     RARDGGAVVRFSGLSREMLILATLLLFCAVLLIVLVGTLYPMIYGLLGWGRLSVGAPY
                     FNRATLPFGLLMLVVIVLATFVSGKRVQLPALVAHAGVLLFAAGIVVSSVSRQEISLN
                     LQPGQQVTLAGYTFRFERLDLQAKGNYTSEKAIVALFDHQQRIGELMPERRFYEARRQ
                     QMMEPSIRWNGIHDWYAVMGEKTGADRYAFRLYVQSGVRWIWGGGLLMIAGALLSGWR
                     GRKRDE"
     gene            complement(2718..3674)
                     /gene="nrfD"
                     /locus_tag="ACHOSC_RS15720"
                     /old_locus_tag="pgaptmp_003144"
     CDS             complement(2718..3674)
                     /gene="nrfD"
                     /locus_tag="ACHOSC_RS15720"
                     /old_locus_tag="pgaptmp_003144"
                     /EC_number="1.7.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c nitrite reductase subunit NrfD"
                     /protein_id="HGU4549041.1"
                     /translation="MTQTSAFHFESLVWDWPIAIYLFLIGISAGLVTLAVLLRRFYPQ
                     AGGADSTLLRTTLIVGPGAVILGLLILVFHLTRPWTFWKLMFHYSFTSVMSMGVMLFQ
                     LYMVVLVLWLAKIFEHDLLALQQRWLPKLGIVQKVLSLLTPVHRGLETLMLVLAVLLG
                     AYTGFLLSALKSYPFLNNPILPVLFLFSGISSGAAVALIAMAIRQRSNPHSTEAQFVH
                     RMEIPVVWGEIFLLVAFFVGLALGDDGKVRALVAALGGGFWTWWFWLGVAGLGLIVPM
                     LLKPWVNRSSGIPAVLAACGASLVGVLMLRFFILYAGQLTVA"
     gene            complement(3671..4342)
                     /gene="nrfC"
                     /locus_tag="ACHOSC_RS15725"
                     /old_locus_tag="pgaptmp_003145"
     CDS             complement(3671..4342)
                     /gene="nrfC"
                     /locus_tag="ACHOSC_RS15725"
                     /old_locus_tag="pgaptmp_003145"
                     /EC_number="1.7.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c nitrite reductase Fe-S protein"
                     /protein_id="HGU4549042.1"
                     /translation="MTWSRRQFLTGVGVLAAVSGTAGRVVAKTLNINGVRYGMVHDES
                     LCIGCTACMDACREVNKVPEGVSRLTIIRSEPQGEFPDVKYRFFRKSCQHCDHAPCVD
                     VCPTGASFRDAASGIVDVNPDLCVGCQYCIAACPYRVRFIHPVTKTADKCDFCRKTNL
                     QAGKLPACVEACPTKALTFGNLDDPNSEISQLLRQKPTYRYKLALGTKPKLYRVPFKY
                     GEVSQ"
     gene            complement(4339..4905)
                     /gene="nrfB"
                     /locus_tag="ACHOSC_RS15730"
                     /old_locus_tag="pgaptmp_003146"
     CDS             complement(4339..4905)
                     /gene="nrfB"
                     /locus_tag="ACHOSC_RS15730"
                     /old_locus_tag="pgaptmp_003146"
                     /EC_number="1.7.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418495.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c nitrite reductase pentaheme
                     subunit"
                     /protein_id="HGU4549043.1"
                     /translation="MSVLRSLLTAGVLASGLLWSLNGITATPAAQASDDRYEVTQQRN
                     PDAACLDCHKPDTEGMHGKHASVINPNNKLPVTCTNCHGQPSPQHREGVKDVMRFNEP
                     MYKVGEQNSVCMSCHLPEQLQKAFWPHDVHVTKVACASCHSLHPQQDTMQTLSDKGRI
                     KICVDCHSDQRTNPNFNPASVPLLKEQP"
     gene            complement(4950..6386)
                     /gene="nrfA"
                     /locus_tag="ACHOSC_RS15735"
                     /old_locus_tag="pgaptmp_003147"
     CDS             complement(4950..6386)
                     /gene="nrfA"
                     /locus_tag="ACHOSC_RS15735"
                     /old_locus_tag="pgaptmp_003147"
                     /EC_number="1.7.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA];
                     GO_function: GO:0005509 - calcium ion binding [Evidence
                     IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_function: GO:0042279 - nitrite reductase (cytochrome,
                     ammonia-forming) activity [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ammonia-forming nitrite reductase cytochrome
                     c552 subunit"
                     /protein_id="HGU4549044.1"
                     /translation="MTRIKINARRIFSLLIPFFFFTSVHAEQTAAPAKPVTVEAKNET
                     FAPQHPDQYLSWKATSEQSERVDALAEDPRLVILWAGYPFSRDYNKPRGHAFAVTDVR
                     ETLRTGAPKNAEDGPLPMACWSCKSPDVARLIQKDGEDGYFHGKWARGGPEIVNNLGC
                     ADCHNTASPEFAKGKPELTLSRPYAARAMEAIGKPFEKAGRFDQQSMVCGQCHVEYYF
                     DGKNKAVKFPWDDGMKVENMEQYYDKIAFSDWTNSLSKTPMLKAQHPEYETWTAGIHG
                     KNNVTCIDCHMPKVQNAEGKLYTDHKIGNPFDNFAQTCANCHTQDKAALQKVVAERKQ
                     SINDLKIKVEDQLVHAHFEAKAALDAGATEAEMKPIQDDIRHAQWRWDLAIASHGIHM
                     HAPEEGLRMLGTAMDKAADARTKLARLLATKGITHEIQIPDISTKEKAQQAIGLNMEQ
                     IKAEKQDFIKTVIPQWEEQARKNGLLSQ"
     gene            6778..8736
                     /gene="acs"
                     /locus_tag="ACHOSC_RS15740"
                     /old_locus_tag="pgaptmp_003148"
     CDS             6778..8736
                     /gene="acs"
                     /locus_tag="ACHOSC_RS15740"
                     /old_locus_tag="pgaptmp_003148"
                     /EC_number="6.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003987 - acetate-CoA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0016208 - AMP binding [Evidence IEA];
                     GO_process: GO:0019427 - acetyl-CoA biosynthetic process
                     from acetate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetate--CoA ligase"
                     /protein_id="HGU4549045.1"
                     /translation="MSQIHKHTIPANIADRCLINPQQYEAMYQQSINVPDTFWGEQGK
                     ILDWIKPYQKVKNTSFAPGNVSIKWYEDGTLNLAANCLDRHLQENGDRTAIIWEGDDA
                     SQSKHISYKELHRDVCRFANTLLELGIKKGDVVAIYMPMVPEAAVAMLACARIGAVHS
                     VIFGGFSPEAVAGRIIDSNSRLVITSDEGVRAGRSIPLKKNVDDALKNPNVTSVEHVV
                     VLKRTGGKIDWQEGRDLWWHDLVEQASDQHQAEEMNAEDPLFILYTSGSTGKPKGVLH
                     TTGGYLVYAALTFKYVFDYHPGDIYWCTADVGWVTGHSYLLYGPLACGATTLMFEGVP
                     NWPTPARMAQVVDKHQVNILYTAPTAIRALMAEGDKAIEGTDRSSLRILGSVGEPINP
                     EAWEWYWKKIGNEKCPVVDTWWQTETGGFMITPLPGATELKAGSATRPFFGVQPALVD
                     NEGNPLEGATEGSLVITDSWPGQARTLFGDHERFEQTYFSTFKNMYFSGDGARRDEDG
                     YYWITGRVDDVLNVSGHRLGTAEIESALVAHPKIAEAAVVGIPHNIKGQAIYAYVTLN
                     HGEEPSPELYAEVRNWVRKEIGPLATPDVLHWTDSLPKTRSGKIMRRILRKIAAGDTS
                     NLGDTSTLADPGVVEKLLEEKQAIAMPS"
     gene            8937..9251
                     /gene="yjcH"
                     /locus_tag="ACHOSC_RS15745"
                     /old_locus_tag="pgaptmp_003149"
     CDS             8937..9251
                     /gene="yjcH"
                     /locus_tag="ACHOSC_RS15745"
                     /old_locus_tag="pgaptmp_003149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF485 domain-containing protein"
                     /protein_id="HGU4549046.1"
                     /translation="MNGTIYQRIEDNAHFRELVEKRQRFATILSIIMLAVYIGFILLI
                     AFAPGWLGTPLNPNTSVTRGIPIGVGVIVISFVLTGIYIWRANGEFDRLNNEVLHEVQ
                     AS"
     gene            9248..10897
                     /gene="actP"
                     /locus_tag="ACHOSC_RS15750"
                     /old_locus_tag="pgaptmp_003150"
     CDS             9248..10897
                     /gene="actP"
                     /locus_tag="ACHOSC_RS15750"
                     /old_locus_tag="pgaptmp_003150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015123 - acetate transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0015370 - solute:sodium symporter activity
                     [Evidence IEA];
                     GO_function: GO:0031402 - sodium ion binding [Evidence
                     IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation/acetate symporter ActP"
                     /protein_id="HGU4549047.1"
                     /translation="MKRVLTALAATLPFAANAADAISGAVERQPTNWQAIIMFLIFVV
                     FTLGITYWASKRVRSRSDYYTAGGNITGFQNGLAIAGDYMSAASFLGISALVFTSGYD
                     GLIYSLGFLVGWPIILFLIAERLRNLGRYTFADVASYRLKQGPIRILSACGSLVVVAL
                     YLIAQMVGAGKLIELLFGLNYHIAVVLVGVLMMMYVLFGGMLATTWVQIIKAVLLLFG
                     ASFMAFMVMKHVGFSFNNLFSEAMAVHPKGVDIMKPGGLVKDPISALSLGLGLMFGTA
                     GLPHILMRFFTVSDAREARKSVFYATGFMGYFYILTFIIGFGAIMLVGANPEYKDAAG
                     HLIGGNNMAAVHLANAVGGNLFLGFISAVAFATILAVVAGLTLAGASAVSHDLYANVF
                     KKGATEREELRVSKITVLILGVIAIILGVLFENQNIAFMVGLAFAIAASCNFPIILLS
                     MYWSKLTTRGAMMGGWLGLITAVVLMILGPTIWVQILGHEKAIFPYEYPALFSISVAF
                     LGIWFFSATDNSAEGARERELFRAQFIRSQTGFGVEQGRAH"
BASE COUNT         2515 a         3073 c         2911 g         2436 t
ORIGIN      
        1 caccagatga ttaatctgat ctccgctgca acaatttcgc catattaatc gactcgacca
       61 gctgtgtccg gttgatccgg ggtgagttga gatccatcac tttttgccat agttcgatgg
      121 cttgcgcgta attcgcctgc ataaacgcat cggaagccag cagcatcaga gcggtgattt
      181 cgttagagtc cagcgcgagg gctttgtcga tcattgcgcg agtctgggcg gtcatatgct
      241 ggctggcctg gtaataaagc accgtcgcca gcgccgcata cagttcagcg ttctcaccac
      301 gtagttgcaa cgcctgacgg tacgccagca gcgaattgct gtaatcgttt tgccacagat
      361 agtactcgcc cagtaacgcc cattgttcgc tgttttgtgg attagcgcgg attttatcct
      421 gcaatgcctg aagctgcgct tccggggttt gctggctggc aaactgatgt agcggatcgc
      481 gctgacgctg atattccgca tgtaccgcct gccattttgg acttaacaga taactgccga
      541 cacagagaaa taccatcaaa atcgtgagtg ttgtcagcat tttcaccggt attttgggct
      601 ctttcatcgc ttcgccctca ctcgccagag gatcagtgcc atcagccgta acaacaccac
      661 tggcagcgcc cacaacacca gcgtctgacc cgttaacggt gggttatagc gaacaaaatc
      721 tccgtagcgt tcggtcatcc agccaatgat ttcgacttcg ttcttcccct ccgccaccat
      781 gctgtaaacc tgatggcgca tactgactgc caccggcgcg ttggattcca gtaagttttg
      841 attctggcac tgcggacaac gtaactggct ggcaatattt aacgcctgtt gctgttgttg
      901 cggattggcg aattgccagg tgtctacgac ctgagcgcgc gcaaaacagg taaataacag
      961 cagtagcgtg agaagtcctt tattcatcgc gcttcctccc ccgccatccg cttaacaatg
     1021 cgcccgcaat catcaacaat cctccccccc agatccagcg cacaccgctt tgtacataca
     1081 agcgaaaagc gtaacgatcc gctccggttt tttcacccat gaccgcatac cagtcatgga
     1141 tgccgttcca gcgaattgac ggttccatca tttgctgacg acgtgcttcg taaaaacgcc
     1201 gctccggcat cagttcacca atgcgttgct gatggtcaaa cagtgccact atcgcttttt
     1261 cgctggtgta attgcctttg gcttgcagat cgaggcgctc aaaacggaag gtgtatcctg
     1321 ccagcgtcac ctgctgaccc ggctgtaaat tcaggctgat ctcctgacgg ctgacgctgg
     1381 aaaccacgat ccccgcggca aataacagca cgcccgcatg agctaccagc gccggaagct
     1441 gcacgcgttt gccagagaca aacgtcgcca gcacaatcac caccagcatc aacaggccaa
     1501 acggtaacgt cgcgcggtta aaatacggcg cgccaacgga gaggcgtccc cagcccagca
     1561 ggccgtaaat catcggataa agcgttccca ccagcacgat cagtagcact gcgcaaaaca
     1621 gcaacagcgt agcgaggatt aacatttccc gcgataaccc cgaaaaacgc accaccgcac
     1681 caccgtcccg cgctcgccag ccatacagag ccagagacgc aaggctaatc agtgcaaaca
     1741 ggctgaacaa cggcacggcg cggacgttat ccagtgcgaa cgcatgaacc gaaaccagaa
     1801 tgccagaacg gacaattaag gtacccagta gcgacagcat cagagttact atcgccagca
     1861 acagcgacca gtggcggaaa atctcccgct ggcgtgtcag ggacaaactg tgcagcagcg
     1921 cagtggcaga aagccagggt aataaagagg cgttttccac cggatcccag aaccaccagc
     1981 cgccccagcc cagttcgcaa taggcccacc aggaaccgag gatgatcccc gccgttaatg
     2041 cgctccagcc aggtagtgcc cagcgccaac aaattctggc gcaggcacca tcaaactcgc
     2101 cgcgcagtaa actcgccagc gccacgctcg ccgctaccat caaaccgcca tagccaaggt
     2161 aaagcagcgg tggatgaaag ataagaccgg gatgttgcag catcggattg agatcgcggc
     2221 cttcgattgc tggtggaaat atccgcacga agggatcgga ccacagcacc acaaacagta
     2281 gcagtgcggc gagcatgaga gataaaacgg ctaacgtcag cggaaatagc ggatcggttt
     2341 gctgccgata atgccaggca aacagcgcgc tccaggcgga aagcagcagc acccaaagca
     2401 gcagcgaacc ttcatggccg ccccacaccg ctgccagctt gagttcccac gacagcaggc
     2461 tatagctatg ttgcgcgaca taaatgaccg agaaatcgct gatgagaaaa caatacgtca
     2521 gtacgccaaa ggcgagcagc aggagcgcga actgcgccag aatgccgatg caagtgagtc
     2581 gcatcatggc aggccagcgc aaccgcactc ccgcaaaggc cgtcaacggg gtcaacacgt
     2641 tgaccccgag acttaacaac aacgccagaa aaccgacttc aggaaggaat acgtccagaa
     2701 accacctctt ttctggctta cgccaccgtc aactgtccgg cgtagagaat gaaaaagcgc
     2761 agcatcaaca cgccgaccag actggcccca cacgccgcca gcacggcagg aatgccggaa
     2821 ctgcgattga cccacggttt gagcaacatt ggcacaatca gccccagccc ggcgacacca
     2881 agccagaacc accacgtcca gaaaccgcca cctaatgccg ccaccagcgc acgcacttta
     2941 ccgtcatcgc ccagcgccag accgacaaaa aatgccacca gcaggaagat ttcaccccat
     3001 accacgggga tttccatacg gtgtacaaac tgcgcttccg tggaatgcgg gttactgcgt
     3061 tggcgtatcg ccatggcgat cagcgccacc gccgcaccgg acgagatgcc ggagaagagg
     3121 aacagcaccg gcaggatcgg gttattgagg aacggatacg atttcagcgc cgacagcaga
     3181 aagccggtat aagcccccaa caacaccgcc aacaccagca tcaatgtttc cagtccgcga
     3241 tgaacgggcg tcagcaggct cagaaccttt tgcacgatcc ccagcttcgg caaccagcgt
     3301 tgttgcaggg caagcaaatc atgttcaaag attttcgcca gccacagcac cagcaccacc
     3361 atgtagagct gaaacagcat cacccccatc gacatcaccg aggtaaaact gtagtggaac
     3421 atcagcttcc agaaggtcca cggtcttgtc aggtggaaga cgaggatcaa cagaccgagg
     3481 atcaccgcgc ccggcccgac aatcagcgtg gtgcgcagca acgtactgtc tgcaccgccc
     3541 gcctgcgggt agaagcgacg taacagcacg gccagcgtca ccagaccggc agaaatacca
     3601 atcaaaaaca ggtagatggc aatcggccag tcccacacca gcgattcaaa atgaaatgcg
     3661 gaagtctgcg tcattggctc acctccccgt atttaaacgg tacgcggtac agcttcggtt
     3721 tggttcccag cgccagcttg tagcggtaag taggcttctg gcgcagcagt tgcgaaatct
     3781 cactgttagg atcgtccaga ttgccaaacg tcagcgcctt cgtcgggcaa gcctcaacgc
     3841 acgcgggcag cttaccggcc tgcaaattgg ttttacggca gaaatcgcat ttgtccgccg
     3901 tcttcgtgac cggatggata aagcgcacgc ggtacggaca ggcggcgatg cagtactgac
     3961 aaccgacgca gagatcagga ttaacatcga caatcccgct agcggcatca cgaaaagacg
     4021 caccggtcgg gcagacgtca acgcacggcg catgatcgca gtgctggcaa gacttacgga
     4081 agaagcgata tttcacatca ggaaattcgc cctgcggctc gctacgaata atcgtcaggc
     4141 gcgagacgcc ttccggcacc ttgttcactt cccgacaagc atccatacag gcggtacagc
     4201 cgatgcataa cgactcgtca tgcaccatgc cgtaacgcac cccattgata ttcaacgttt
     4261 tcgccaccac gcgccctgcg gtgccgctga cggctgccag cacgccgacg ccggtgagaa
     4321 actggcgacg agaccaggtc atggctgctc cttaagcaac ggaacagacg ccgggttaaa
     4381 gttcggattg gtgcgctgat cgctgtggca atcgacgcaa atcttaatcc gtcctttgtc
     4441 acttaacgtc tgcatcgtat cttgctgtgg atgcagggaa tggcagctgg cgcacgccac
     4501 tttggttacg tggacatcgt gcggccagaa cgctttttgc aactgttccg gcagatgaca
     4561 ggacatacag acgctgttct gctctccaac cttgtacatc ggctcgttaa agcgcatcac
     4621 atctttcacc ccttcgcggt gttgcggtga tggctggccg tgacagttgg tgcaggtgac
     4681 cggcagtttg ttattcgggt tgataacgga agcatgtttt ccatgcatac cttcggtatc
     4741 tggtttatga cagtccagac aggcggcatc cgggttacgc tgctgggtaa cttcgtagcg
     4801 atcatcagat gcctgcgccg caggggtagc ggtaattccg ttcaggctcc acaacaagcc
     4861 cgacgccagc accccggcag ttaataacga acgtaatacg ctcatattca ctccattgtg
     4921 ttttccgccc cttgcgaggc ggaacggggt tattggctta acagaccatt tttacgtgcc
     4981 tgttcttccc actgcgggat caccgttttg atgaagtcct gcttctcggc cttgatttgt
     5041 tccatgttca ggccaatggc ctgctgggct ttctctttgg tcgagatatc cgggatctgg
     5101 atttcatggg tgatgccttt ggtcgccagc aggcgcgcca gtttggtgcg tgcatccgcc
     5161 gctttatcca tcgccgtacc gagcatccgt agaccttctt ccggtgcgtg catatgaata
     5221 ccgtgggaag cgatcgccag atcccaacgc cactgggcat gacggatatc gtcctgaatc
     5281 ggcttcattt cggcttccgt cgcgcctgca tccagcgctg ctttcgcttc gaagtgagcg
     5341 tgaaccagtt gatcttcaac cttgattttc aggtcgttaa tcgactgctt acgttccgcg
     5401 accacttttt gcaaagcagc tttgtcctgg gtatggcagt tcgcacaagt ctgggcgaag
     5461 ttatcaaacg gattaccaat tttatggtcg gtgtagagtt tgccttcggc gttctgcact
     5521 tttggcatat ggcagtcgat acaggtcacg ttgtttttac cgtgaatgcc cgctgtccag
     5581 gtttcatatt ccgggtgctg cgctttcagc attggcgttt tcgacaggga gttagtccag
     5641 tcagagaagg caattttgtc gtaatactgc tccatatttt cgactttcat gccgtcatcc
     5701 cacgggaatt taaccgcttt gtttttgccg tcgaagtaat actccacatg gcactgaccg
     5761 caaaccatcg attgctggtc gaaacgtccg gctttctcaa aaggtttacc aatggcttcc
     5821 atcgcgcgag ccgcatacgg acgggaaagg gttaactccg gtttgccttt ggcgaactct
     5881 ggtgaggcgg tgttatggca gtcggcacaa cctaagttgt tgacgatttc cgggccgccg
     5941 cgcgcccact taccgtggaa gtagccatct tcgccgtctt tctggatcag acgcgccaca
     6001 tccgggcttt tacaactcca gcacgccatc ggtagcggac catcttcagc gtttttcggc
     6061 gcaccggtac gcagggtttc acgcacatcg gtcacagcaa aggcatgtcc acgcggcttg
     6121 ttgtaatcgc gcgagaaggg ataccccgcc cacaggatca ccagccgtgg atcttccgcc
     6181 agggcgtcaa cacgctctga ctgttccgag gtggctttcc aggagagata ttgatcggga
     6241 tgctgcgggg caaaggtttc attcttcgct tccacagtta caggttttgc gggagctgcc
     6301 gtttgttcag cgtgaacaga agtgaaaaag aaaaaaggaa tcaataagct gaagatacgg
     6361 cgtgcgttta tttttatcct tgtcataggg gcttcatccg aattgcgcca ttgttgcaat
     6421 ggcggttttt attgttttca cgacagtaac cgcacctaca ctgtcatgac attgctcgcc
     6481 cctatgtgta acatataacc acactgtgaa tgttgtcttt aatcaattgt aagtgcatgt
     6541 aaaataccac tttagagtta gtcagtatct tcctcttttt caacagcatg cataactgca
     6601 tgttcctcaa agaattaatc aacttttgtt ggtgaccttc aaaaattacc ctgccgttta
     6661 tttgcacaat tctacttttg cgtgatctgt cgcccaaata ctaaacaaaa ctgccaatac
     6721 ccctacattt aacgcttatg ccacatatta ttaacatcct acaaggagaa caaaagcatg
     6781 agccaaattc acaaacacac cattcctgcc aacatcgcag accgttgcct gataaaccct
     6841 cagcagtacg aggcgatgta tcaacaatct attaacgtac ctgatacctt ctggggcgaa
     6901 cagggaaaaa ttctcgactg gatcaaaccg taccagaagg tgaaaaatac ctcctttgcc
     6961 cccggtaatg tgtccattaa atggtacgag gacggcacgc tgaatctggc ggcaaactgc
     7021 cttgaccgcc atctgcaaga aaacggcgat cgcaccgcca tcatctggga aggcgacgac
     7081 gccagccaga gcaaacatat cagctataaa gagctgcacc gcgacgtgtg ccgcttcgcc
     7141 aataccctgc tcgagctggg cattaaaaaa ggtgatgtgg tggcgattta tatgccgatg
     7201 gtgccggaag ccgcggttgc gatgctggcc tgcgcccgca ttggcgcggt gcattcggtg
     7261 attttcggcg gcttctcgcc ggaagccgtt gccgggcgca ttattgattc caactcacga
     7321 ctggtgatca cttccgacga aggtgtgcgt gccgggcgca gtattccgct gaagaaaaac
     7381 gttgatgacg cgctgaaaaa cccgaacgtc accagcgtag agcatgtggt ggtactgaag
     7441 cgtactggcg ggaaaattga ctggcaggaa gggcgcgacc tgtggtggca cgacctggtt
     7501 gagcaagcga gcgatcagca ccaggcggaa gagatgaacg ccgaagatcc gctgtttatt
     7561 ctctacacct ccggttctac cggtaagcca aaaggtgtgc tgcatactac cggcggttat
     7621 ctggtgtacg cggcgctgac ctttaaatat gtctttgatt atcatccggg tgatatctac
     7681 tggtgcaccg ccgatgtggg ctgggtgacc ggacacagtt acttgctgta cggcccgctg
     7741 gcctgcggtg cgaccacgct gatgtttgaa ggcgtaccca actggccgac gcctgcccgt
     7801 atggcgcagg tggtggacaa gcatcaggtc aatattctct ataccgcacc cacggcgatc
     7861 cgcgcgctga tggcggaagg cgataaagcg atcgagggta ccgaccgttc gtcgctgcgc
     7921 attctcggtt ccgtgggcga gccaattaac ccggaagcgt gggagtggta ctggaaaaaa
     7981 atcggcaacg agaaatgtcc ggtggtcgat acctggtggc agaccgaaac tggcggtttc
     8041 atgatcacgc cgctgcctgg cgctaccgag ctgaaagccg gttcggcaac acgtccgttc
     8101 ttcggcgtgc aaccggcgct ggtcgataac gaaggtaacc cgctggaagg cgctaccgaa
     8161 ggcagcctgg tgatcaccga ctcctggccg ggtcaggcgc gtacgctgtt tggcgatcac
     8221 gaacgttttg aacagaccta cttctccacc ttcaaaaata tgtatttcag cggcgacggc
     8281 gcgcgtcgcg atgaagatgg ctattactgg atcaccgggc gtgtggacga cgtgctgaac
     8341 gtctccggtc accgtctggg gacggcagag attgagtcgg cgctggtggc gcatccgaag
     8401 attgccgaag ccgccgtagt aggtattccg cacaatatta aaggtcaggc gatctacgcc
     8461 tacgtcacgc ttaatcacgg ggaggaaccg tcaccagaac tgtacgcaga agtccgcaac
     8521 tgggtgcgta aagagattgg cccgctggcg acgccagacg tgctgcactg gaccgactcc
     8581 ctgcctaaaa cccgctccgg caaaattatg cgccgtattc tgcgcaaaat tgcggcgggc
     8641 gataccagca acctgggcga tacctcgacg cttgccgatc ctggcgtagt cgagaagctg
     8701 cttgaagaga agcaggctat cgcgatgcca tcgtaaccca caattgccgg atgcgagtcg
     8761 gtaacggttt gtaggcctga taagacgcga cagcgtcgca tcaggcattg attgccggat
     8821 gcggcgtata acgccttatc cggcctacat tcggcaaggg ttacccgagc gttaaccttc
     8881 tcccataagg gagcgggaat taaaacaatc cctacattac ctctggagaa tctgtgatga
     8941 atggtacgat ttatcagcgg atagaagaca atgcgcattt cagggagtta gtcgaaaaac
     9001 ggcaacggtt tgccaccatc ctgtcgatta ttatgctggc agtttatatc ggctttattt
     9061 tactgatcgc cttcgcgccc ggctggctgg gcacgccgct gaatccgaac accagcgtca
     9121 cacgcggtat tccaattggt gttggagtga ttgtgatctc ctttgttctc accggtatct
     9181 acatctggcg ggcgaacggc gaattcgacc gtcttaataa tgaagtcctg catgaggtac
     9241 aagcatcatg aaaagagttc tgacggcgct tgccgccaca ctccctttcg cagctaacgc
     9301 cgcggatgct attagcgggg ccgtagagcg ccagccaacg aactggcagg cgattattat
     9361 gttcctgatt ttcgtcgtgt ttacgctcgg cattacctac tgggcatcaa aacgcgtacg
     9421 ttctcgtagc gactactaca ccgcaggcgg caatatcact ggcttccaga acgggctggc
     9481 gattgccggg gactatatgt ccgccgcctc attcttgggg atctccgcgc tggtgtttac
     9541 ctccggctat gacggcttaa tttactcgct gggcttcctg gtgggctggc cgatcatttt
     9601 gttcctgatt gccgaacgtc tgcgtaacct ggggcgctac acctttgccg atgtggcctc
     9661 ttaccgtctg aaacaagggc cgattcgtat tctttcggcc tgtggttctc tggtggtggt
     9721 ggcgctttac cttatcgccc agatggtggg cgcaggtaaa ctgatcgagc tgctgtttgg
     9781 ccttaactat cacattgcgg tggtgctggt cggcgtgctg atgatgatgt acgtcctgtt
     9841 cggcggcatg ctggcgacca cttgggtaca gattatcaaa gccgtgctgt tgctgttcgg
     9901 tgccagcttt atggccttta tggtgatgaa acacgtcggc tttagcttca acaatctgtt
     9961 cagtgaagcg atggcggtac acccgaaagg tgtcgacatc atgaagccgg gcgggctggt
    10021 gaaagatccg atctccgcgc tctctctggg tctgggactg atgtttggta cggcgggctt
    10081 gccgcacatt ctgatgcgct tctttacagt cagcgatgcc cgcgaagcac gtaagagcgt
    10141 gttctacgcc accgggttta tgggctactt ctatattctg acctttatta tcggcttcgg
    10201 cgcgatcatg ctggttggtg cgaatccgga atataaagac gcggcgggcc atctgattgg
    10261 tggtaacaac atggcggccg ttcacctggc gaatgcagtg ggcggcaacc tgttcctcgg
    10321 ttttatttca gcggttgctt ttgccaccat tcttgcggtg gttgctggtc tgacgctggc
    10381 gggcgcatca gcggtttcgc atgacctgta cgctaacgtc ttcaaaaaag gcgcgaccga
    10441 acgtgaagaa ctgcgggtat cgaaaatcac cgtgctgatt ttaggtgtga ttgccattat
    10501 cctcggcgtg ctgtttgaga atcagaacat cgccttcatg gtaggcctgg cgtttgccat
    10561 cgcagcgagc tgtaacttcc cgatcattct gctttctatg tactggtcga aactgaccac
    10621 tcgtggcgcg atgatgggtg gctggctggg gctgattacc gcagtggtac tgatgatcct
    10681 cggcccgacg atttgggtac agatccttgg tcacgaaaaa gccatcttcc cgtatgaata
    10741 cccggcgctg ttctctatca gcgtggcatt cctcggcatc tggttcttct cggcaaccga
    10801 taactcagcg gaaggtgcgc gcgagcgtga actgttccgt gcacagttta tccgctccca
    10861 gaccggcttt ggcgttgagc aaggccgcgc acattaatct ctccccttcc ccggtcgcct
    10921 gaccggggaa tactc
//
LOCUS       DAZKHF020000150        10908 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2201S31241
            SAMN43267800-rid25106413.denovo.151, whole genome shotgun sequence.
ACCESSION   DAZKHF020000150 DAZKHF020000000
VERSION     DAZKHF020000150.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267800
            Sequence Read Archive: SRR30307263
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 10908)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10908)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 10908)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350581.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350581.2
            Genome Coverage       :: 151x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:21:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,652
            CDSs (total)                      :: 5,557
            Genes (coding)                    :: 5,320
            CDSs (with protein)               :: 5,320
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 237
            CDSs (without protein)            :: 237
            Pseudo Genes (ambiguous residues) :: 0 of 237
            Pseudo Genes (frameshifted)       :: 102 of 237
            Pseudo Genes (incomplete)         :: 130 of 237
            Pseudo Genes (internal stop)      :: 52 of 237
            Pseudo Genes (multiple problems)  :: 41 of 237
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10908
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267800-rid25106413.denovo.151"
                     /serotype="O 26:H11"
                     /isolate="2201S31241"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-01"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            122..625
                     /gene="dps"
                     /locus_tag="ACHOSC_RS15755"
                     /old_locus_tag="pgaptmp_003151"
                     /gene_synonym="pexB"
     CDS             122..625
                     /gene="dps"
                     /locus_tag="ACHOSC_RS15755"
                     /old_locus_tag="pgaptmp_003151"
                     /gene_synonym="pexB"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001730644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016722 - oxidoreductase activity, acting
                     on metal ions [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA starvation/stationary phase protection
                     protein Dps"
                     /protein_id="HGU4549048.1"
                     /translation="MSTAKLVKSKATNLLYTRNDVSDSEKKATVELLNRQVIQFIDLS
                     LITKQAHWNMRGANFIAVHEMLDGFRTALIDHLDTMAERAVQLGGVALGTTQVINSKT
                     PLKSYPLDIHNVQDHLKELADRYAIVANDVRKAIGEAKDDDTADILTAASRDLDKFLW
                     FIESNIE"
     gene            1029..1775
                     /gene="glnH"
                     /locus_tag="ACHOSC_RS15760"
                     /old_locus_tag="pgaptmp_003152"
     CDS             1029..1775
                     /gene="glnH"
                     /locus_tag="ACHOSC_RS15760"
                     /old_locus_tag="pgaptmp_003152"
                     /EC_number="7.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0015599 - ATPase-coupled L-glutamine
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:1903803 - L-glutamine import across plasma
                     membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine ABC transporter substrate-binding
                     protein GlnH"
                     /protein_id="HGU4549049.1"
                     /translation="MKSVLKVSLAALTLAFAVSSHAADKKLVVATDTAFVPFEFKQGD
                     KYVGFDVDLWAAIAKELKLDYELKPMDFSGIIPALQTKNVDLALAGITITDERKKAID
                     FSDGYYKSGLLVMVKANNNDVKSVKDLDGKVVAVKSGTGSVDYAKANIKTKDLRQFPN
                     IDNAYMELGTNRADAVLHDTPNILYFIKTAGNGQFKAVGDSLEAQQYGIAFPKGSDEL
                     RDKVNGALKTLRENGTYNEIYKKWFGTEPK"
     gene            1914..2573
                     /gene="glnP"
                     /locus_tag="ACHOSC_RS15765"
                     /old_locus_tag="pgaptmp_003153"
     CDS             1914..2573
                     /gene="glnP"
                     /locus_tag="ACHOSC_RS15765"
                     /old_locus_tag="pgaptmp_003153"
                     /EC_number="7.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015367581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine ABC transporter permease GlnP"
                     /protein_id="HGU4549050.1"
                     /translation="MQFDWSAIWPAIPLLIEGAKMTLWISVLGLAGGLVIGLLAGFAR
                     TFGGWIANHVALVFIEVIRGTPIVVQVMFIYFALPMAFNDLRIDPFTAAVVTIMINSG
                     AYIAEITRGAVLSIHKGFREAGLALGLSRWETIRYVILPLALRRMLPPLGNQWIISIK
                     DTSLFIVIGVAELTRQGQEIIAGNFRALEIWSAVAVLYLIITLVLSFILRRLERRMKI
                     L"
     gene            2570..3292
                     /gene="glnQ"
                     /locus_tag="ACHOSC_RS15770"
                     /old_locus_tag="pgaptmp_003154"
     CDS             2570..3292
                     /gene="glnQ"
                     /locus_tag="ACHOSC_RS15770"
                     /old_locus_tag="pgaptmp_003154"
                     /EC_number="7.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000569103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015424 - ABC-type amino acid transporter
                     activity [Evidence IEA];
                     GO_process: GO:0003333 - amino acid transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine ABC transporter ATP-binding protein
                     GlnQ"
                     /protein_id="HGU4549051.1"
                     /translation="MIEFKNVSKHFGPTQVLHNIDLNIAQGEVVVIIGPSGSGKSTLL
                     RCINKLEEITSGDLIVDGLKVNDPKVDERLIRQEAGMVFQQFYLFPHLTALENVMFGP
                     LRVRGANKEEAEKLARELLAKVGLAERAHHYPSELSGGQQQRVAIARALAVKPKMMLF
                     DEPTSALDPELRHEVLKVMQDLAEEGMTMVIVTHEIGFAEKVASRLIFIDKGRIAEDG
                     DPQVLIKNPPSQRLQEFLQHVS"
     gene            3409..5634
                     /gene="ybiO"
                     /locus_tag="ACHOSC_RS15775"
                     /old_locus_tag="pgaptmp_003155"
     CDS             3409..5634
                     /gene="ybiO"
                     /locus_tag="ACHOSC_RS15775"
                     /old_locus_tag="pgaptmp_003155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415329.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive channel protein"
                     /protein_id="HGU4549052.1"
                     /translation="MRWILFILFCLLGAPAHAVSIPGVTTTTTTDSTTEPAPEPDIEQ
                     KKAAYGALADVLDNDTSRKELIDQLRTVAATPPAEPVPKIVPPTLVEEQTVLQKVTEV
                     SRHYGEALSARFGQLYRNITGSPHKPFNPQTFSNALSHFSMLAVLVFGFYWLIRLCAL
                     PLYRKMGQWARQKNRERSNWLQLPAMIIGAFIIDLLLLALTLFVGQVLSDNLNAGSRT
                     IAFQQSLFLNAFALIEFFKAVLRLIFCPNVAELRPFTIQDESARYWSRRLSWLSSLIG
                     YGLIVAVPIISNQVNVQIGALANVIIMLCMTVWALYLIFRNKKEITQHLLNFAEHSLA
                     FFSLFIRAFALVWHWLASAYFIVLFFFSLFDPGNSLKFMMGATVRSLAIIGIAAFVSG
                     MFSRWLAKTITLSPHTQRNYPELQKRLNGWLSAALKTARILTVCVAVMLLLSAWGLFD
                     FWNWLQNGAGQKTVDILIRIALILFFSAVGWTVLASLIENRLASDIHGRPLPSARTRT
                     LLTLFRNALAVIISTITIMIVLSEIGVNIAPLLAGAGALGLAISFGSQTLVKDIITGV
                     FIQFENGMNTGDLVTIGPLTGTVERMSIRSVGVRQDTGAYHIIPWSSITTFANFVRGI
                     GSVVANYDVDRHEDADKANQALKDAVAELMENEEIRGLIIGEPNFAGIVGLSNTAFTL
                     RVSFTTLPLKQWTVRFALDSQVKKHFDLAGVRAPVQTYQVLPAPGATPAEPPPPGEPT
                     L"
     gene            complement(5631..6557)
                     /gene="rlmF"
                     /locus_tag="ACHOSC_RS15780"
                     /old_locus_tag="pgaptmp_003156"
     CDS             complement(5631..6557)
                     /gene="rlmF"
                     /locus_tag="ACHOSC_RS15780"
                     /old_locus_tag="pgaptmp_003156"
                     /EC_number="2.1.1.181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415328.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008988 - rRNA
                     (adenine-N6-)-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(1618)-N(6))-methyltransferase
                     RlmF"
                     /protein_id="HGU4549053.1"
                     /translation="MSAQKPGLHPRNRHHSRYDLATLCQVNPELRQFLTLTPAGEQSV
                     DFANPLAVKALNKALLAHFYAVANWDIPDGFLCPPVPGRADYIHHLADLLAEASGTIP
                     ANASILDIGVGANCIYPLIGVHEYGWRFTGSETSSQALSSAQAIISANPGLNRAIRLR
                     RQKESGAIFNGIIHKNEQYDATLCNPPFHDSAAAARAGSERKRRNLGLNKDDALNFGG
                     QQQELWCEGGEVAFIKKMIEESKGFAKQVMWFTSLVSRGENLPPLYRALTDVGAVKVV
                     KKEMAQGQKQSRFIAWTFMNDEQRRRFVNRQR"
     gene            6833..7093
                     /gene="mcbA"
                     /locus_tag="ACHOSC_RS15785"
                     /old_locus_tag="pgaptmp_003157"
     CDS             6833..7093
                     /gene="mcbA"
                     /locus_tag="ACHOSC_RS15785"
                     /old_locus_tag="pgaptmp_003157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415327.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1471 family periplasmic protein McbA"
                     /protein_id="HGU4549054.1"
                     /translation="MKKCLTLLIATVLSGISLTAYAAQPMSNLDSGQLRPAGTVSATG
                     ASNLSDLEDKLAEKAREQGAKGYVINSAGGNDQMFGTATIYK"
     gene            7357..9639
                     /locus_tag="ACHOSC_RS15790"
                     /old_locus_tag="pgaptmp_003158"
     CDS             7357..9639
                     /locus_tag="ACHOSC_RS15790"
                     /old_locus_tag="pgaptmp_003158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015344 - siderophore uptake transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015891 - siderophore transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="catecholate siderophore receptor Fiu"
                     /protein_id="HGU4549055.1"
                     /translation="MENNRNFPARQFHSLTFFAGLCIGITPVAQALAAEGQTNADDTL
                     VVEASTPSLYAPQQSADPKFSRPVADTTRTMTVISEQVIKDQGATNLTDALKNVPGVG
                     AFFAGENGNSTTGDAIYMRGADTSNSIYIDGIRDIGSVSRDTFNTEQVEVIKGPSGTD
                     YGRSAPTGSINMISKQPRNDSGIDASASIGSAWFRRGTLDVNQVIGDTTAVRLNVMGE
                     KTHDAGRDKVKNERYGVAPSIAFGLGTANRLYLNYLHVTQHNTPDGGIPTIGLPGYSA
                     PSAGTAALNHSGKVDTHNFYGTDSDYDDSTTDTATMRFEHDINDNTTIRNTTRWSRVK
                     QDYLMTAIMGGASNITQPTSDVNSWTWSRTANTKDVSNKILTNQTNLTSTFYTGSIGH
                     DVSTGVEFTRETQTNYGVNPVTLPAVNIYHPDSSIHPGGLTRNGANANGQTDTFAIYA
                     FDTLQITRDFELNGGIRLDNYHTEYDSATACGGSGRGAITCPTGVAKGSPVTTVDTAK
                     SGNLVNWKAGALYHLTENGNAYINYAVSQQPPGGNNFALAQSGSGNSANRTDFKPQKA
                     NTSEIGTKWQVLDKRLLLTAALFRTDIENEVEQNDDGTYSQYGKKRVEGYEISVAGNI
                     TPAWQVIGGYTQQKATIKNGKDVAQDGSSSLPYTPEHAFTLWSQYQATDDISVGAGAR
                     YIGSMHKGSDGAVGTPAFTEGYWVADAKLGYRVNRNLDFQLNVYNLFDTDYVASINKS
                     GYRYHPGEPRTFLLTANMHF"
     gene            9681..10358
                     /gene="ybiX"
                     /locus_tag="ACHOSC_RS15795"
                     /old_locus_tag="pgaptmp_003159"
     CDS             9681..10358
                     /gene="ybiX"
                     /locus_tag="ACHOSC_RS15795"
                     /old_locus_tag="pgaptmp_003159"
                     /EC_number="1.14.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415325.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PKHD-type hydroxylase YbiX"
                     /protein_id="HGU4549056.1"
                     /translation="MMYHIPGVLSPQDVARFREQLEQAEWVDGRVTTGAQGAQVKNNQ
                     QVDTRSTLYAALQNEVLNAVNQHALFFAAALPRTLSTPLFNRYQNNETYGFHVDGAVR
                     SHPQNGWMRTDLSATLFLSDPQSYDGGELVVNDTFGQHRVKLPAGDLVLYPSSSLHCV
                     TPVTRGVRVASFMWIQSMIRDDKKRAMLFELDNNIQSLKSRYGESEEILSLLNLYHNL
                     LREWSEI"
     gene            10432..10698
                     /gene="ybiI"
                     /locus_tag="ACHOSC_RS15800"
                     /old_locus_tag="pgaptmp_003160"
     CDS             10432..10698
                     /gene="ybiI"
                     /locus_tag="ACHOSC_RS15800"
                     /old_locus_tag="pgaptmp_003160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C4-type zinc finger protein YbiI"
                     /protein_id="HGU4549057.1"
                     /translation="MASGWANDDAVNEQINSTIEDAIARARGEIPRGESLDECEECGA
                     PIPQARREAIPGVRLCIHCQQEKGLQKPAYTGYNRRGSKDSQLR"
BASE COUNT         2674 a         2843 c         2763 g         2628 t
ORIGIN      
        1 gtttttcacg cttgttacca ctattagtgt gataggaaca gccagaatag cggaacacat
       61 agccggtgct atacttagtc tcgttaatta ctgggacata acatcaagag gatatgaaat
      121 tatgagtacc gctaaattag ttaaatcaaa agcgaccaat ctgctttata cccgcaacga
      181 tgtctccgac agcgagaaaa aagcaacagt agagttgctg aatcgccagg ttatccagtt
      241 tattgatctt tctttgatta ccaaacaagc gcactggaac atgcgcggcg ctaacttcat
      301 tgccgtacat gaaatgctgg atggcttccg caccgcactg atcgatcatc tggataccat
      361 ggcagaacgt gcagtgcagc tgggcggtgt agctctgggg accactcaag ttatcaacag
      421 caaaaccccg ctgaaaagtt acccgctgga catccacaac gttcaggatc acctgaaaga
      481 actggctgac cgttacgcaa tcgtcgctaa tgacgtacgc aaagcgattg gcgaagcgaa
      541 agatgacgac accgcagata tcctgaccgc cgcgtctcgc gacctggata aattcctgtg
      601 gtttatcgag tctaacatcg aataaatcca tcgctgatgg tgcagaactt tagtacccga
      661 taaaagcggc ttcctgacag gaggccgttt tgttttgcag cccacctcaa cgcacttatt
      721 tagtgcatcc atctgctatc tccagctgat taagtaaatt ttttgtatcc acatcatcac
      781 acaatcgtta cataaagatt gttttttcat caggttttac gctaaataat cactgtgttg
      841 agtgcacaat tttagcgcac cagattggtg ccccagaatg gtgcatcttc agggtattgc
      901 cctataaatc gtgcatcacg tttttgccgc atctcgaaaa atcaatgagt tgcaaaactg
      961 gcacgatttt ttcatatatg tgaatgtcac gcaggggatc gtcccgtgga tagaaaaaag
     1021 gaaatgctat gaagtctgta ttaaaagttt cactggctgc actgaccctg gcttttgcgg
     1081 tttcttctca tgccgcggat aaaaaattag ttgtcgcgac ggataccgcc ttcgttccgt
     1141 ttgaatttaa acagggcgat aaatatgtgg gctttgacgt tgatctgtgg gctgccatcg
     1201 ctaaagagct gaagctggat tacgaactga agccgatgga tttcagtggg atcattccgg
     1261 cactgcaaac caaaaacgtc gatctggcgc tggcgggcat taccatcacc gacgagcgta
     1321 aaaaagcgat cgatttctct gacggctact acaaaagcgg cctgttagtg atggtgaaag
     1381 ctaacaataa cgatgtgaaa agcgtgaaag atctcgacgg gaaagtggtt gctgtgaaga
     1441 gcggtactgg ctccgttgat tacgcgaaag caaacatcaa aactaaagat ctgcgtcagt
     1501 tcccgaacat cgataacgcc tatatggaac tgggcaccaa ccgcgcagac gccgttctgc
     1561 acgatacgcc aaacattctg tacttcatca aaaccgccgg taacggtcag ttcaaagcgg
     1621 taggtgactc tctggaagcg cagcaatacg gtattgcgtt cccgaaaggt agcgacgagc
     1681 tgcgtgacaa agtcaacggc gcgttgaaaa ccctgcgcga gaacggaact tacaacgaaa
     1741 tctacaaaaa atggttcggt actgaaccga aataataacg ctacacctgt aaaacgcact
     1801 ggcagttccc tctcccctct ggggagagga ttagggtgag gggcgcaaac ccgctccggg
     1861 gccattaatt accctgaatt tgattattta caccacggta acaggaacaa catatgcagt
     1921 ttgactggag tgccatctgg cctgccattc cgcttctgat tgaaggtgcc aaaatgaccc
     1981 tgtggatttc ggtcctcggt ctggcaggcg gtctggtaat cggattgctg gcaggttttg
     2041 cacgcacctt cggtggttgg atagccaacc acgtcgcgct ggtctttatt gaagtgatcc
     2101 gcggcacacc tatcgtcgtc caggtgatgt ttatctattt cgccctgccg atggcgttta
     2161 acgacttacg catcgaccca tttactgcgg cggtggtcac catcatgatc aactccggcg
     2221 cgtacattgc ggaaatcacg cgtggtgcgg tgctgtctat ccacaaaggt tttcgtgaag
     2281 caggactggc gctcggtctt tcacgttggg aaaccattcg ctacgtcatt ttaccgctgg
     2341 cactgcgtcg tatgctgccg ccgctgggta accagtggat catcagcatt aaagacacct
     2401 cgctgtttat tgtgatcggc gtggcggaac tgacccgtca ggggcaagaa attattgccg
     2461 gtaacttccg cgcccttgag atctggagcg ccgtggcggt gctctatctg attattaccc
     2521 tggtgctgag ctttattctg cgtcgtctgg aaagaaggat gaaaatcctg tgattgaatt
     2581 taaaaacgtc tccaagcact ttggcccaac ccaggtgctg cataatatcg atttgaacat
     2641 tgcccagggc gaagtcgtgg tgattatcgg gccgtccggt tccggtaaat cgaccctgct
     2701 gcgctgcatc aacaaactgg aagaaatcac ctccggcgat ctgattgtcg atggcctgaa
     2761 ggttaacgat ccgaaagttg acgagcgcct gattcgccag gaagcaggta tggtgttcca
     2821 gcagttttac ctcttcccgc atctgacggc gctggaaaac gtcatgtttg gtccgctacg
     2881 cgtgcgtggc gcgaacaaag aagaggcgga aaaactggcg cgtgagctgc tggcgaaagt
     2941 cggtctggca gaacgtgcac atcactaccc ttccgaactt tctggtggtc aacagcagcg
     3001 tgtggcgatt gcccgcgcgc tggcggtgaa gccgaaaatg atgctgtttg atgaaccgac
     3061 ttccgctctt gacccggaac tgcgccatga agtgctgaag gttatgcagg atctggctga
     3121 agaagggatg acgatggtga tcgtgaccca cgaaatcggt tttgccgaga aagtagcttc
     3181 gcggctgatc tttatcgaca aaggccggat tgcggaagat ggcgatccgc aggtattgat
     3241 caagaacccg ccgagccagc gcttgcagga atttttgcag cacgtctctt aacttctctg
     3301 cctccctttc acccgaaagg gaggcacacc agattcctct catttaaaat cgcccctccc
     3361 ccagcatcta tacttatctt tttgctctgt tttctcactg gaggagtcat gcggtggatc
     3421 ctgttcatcc tcttctgcct gctgggcgca cctgcccacg cggtatccat acccggcgtt
     3481 acaaccacaa cgacaacgga ctcaacgact gaaccggccc cggaaccgga tatcgaacaa
     3541 aaaaaagcgg cctatggcgc actggcggat gtgctggata acgacacctc gcgtaaagaa
     3601 ttgatcgacc agttgcgtac tgtcgccgcg acgccccctg cggaaccggt cccgaagatc
     3661 gtgccgccga cgctggttga agagcaaacc gtgctgcaaa aggtcaccga agtcagccgc
     3721 cattatggtg aagccctttc cgcccgcttc gggcaacttt atcgcaatat caccggctcc
     3781 ccgcataagc catttaatcc acaaaccttc agcaatgcgc tgagccattt ttcaatgtta
     3841 gcggtattag tgtttggttt ttactggctg attcgcctgt gcgcactgcc gctgtatcgc
     3901 aaaatgggtc agtgggcgag gcaaaaaaat cgtgagcgca gcaactggtt gcagcttccg
     3961 gcgatgatta tcggggcgtt tattatcgac ctgctgttac tggccctgac attatttgtc
     4021 ggccaggtat taagcgacaa cctgaatgca ggcagtcgca ccatcgcttt ccaacaaagt
     4081 ttgtttctca atgcctttgc cctgattgaa tttttcaaag ccgtactacg cctgattttt
     4141 tgcccaaacg tggcggagct gcgtccgttc acgattcagg acgagagcgc ccgttactgg
     4201 agtcgtcgcc tgagctggtt aagcagtttg ataggttatg gcctgattgt ggccgtgccg
     4261 attatctcta atcaggtgaa tgtacagata ggtgcgctgg cgaacgtcat cattatgctg
     4321 tgcatgaccg tctgggcgtt gtacctgatc tttcgtaata aaaaagagat tacccagcat
     4381 ttgctcaact ttgcggagca ttcgctggcc tttttcagcc tgtttatccg cgcctttgcg
     4441 ctggtgtggc actggctggc aagcgcctat tttatcgtgc tgtttttctt ttcgttgttc
     4501 gatccgggca acagcctgaa atttatgatg ggtgcaacgg tgcgcagcct ggcgattatt
     4561 ggtatcgcag cgtttgtttc cggtatgttt tcccgctggc tggcgaaaac catcaccctc
     4621 tcgccacata ctcagcgtaa ctatccggag ctacaaaaac ggctgaatgg ctggctttcg
     4681 gcggcgctga aaacggcgcg tattctgacc gtctgcgtgg cagtaatgtt gctgttgagc
     4741 gcatggggat tgttcgattt ctggaactgg ctgcaaaacg gcgcggggca gaaaaccgta
     4801 gatatcctga tccgcatcgc actcattctt ttcttctcgg cggttggctg gacggtgctc
     4861 gccagtttga tcgaaaaccg gctggcttcg gatattcatg gccgcccgct acccagcgcc
     4921 cgtacgcgta ccctgctgac gctgtttcgt aacgcgctgg cggtgattat cagtaccatc
     4981 accatcatga ttgtgttgtc ggaaatcggc gtcaatatcg cgccattgct ggcaggtgcc
     5041 ggggcattag gtctggctat ctcgtttggt tcgcaaacgc tggtgaaaga tatcattacc
     5101 ggggtgttta ttcagtttga aaacggcatg aacactggag atttggtgac tatcgggccg
     5161 ttgaccggca cagtggaacg gatgtcgatt cgctccgtgg gcgtgcgcca ggataccggg
     5221 gcgtatcaca tcattccgtg gtcttcgata accacctttg ctaacttcgt ccgcggtatt
     5281 ggttcggtag tggcaaacta cgatgttgat cgccatgaag atgctgataa agccaatcag
     5341 gcactgaaag atgcggtagc ggaattgatg gaaaacgaag aaattcgcgg gctgattatt
     5401 ggtgaaccga attttgccgg gattgtcggc ttaagcaata ccgcgtttac actgcgtgtt
     5461 tcgttcacca cgctgccact caaacagtgg acggtacgct ttgccctcga cagccaggtg
     5521 aaaaaacatt tcgacctggc gggcgttcgc gcgccagtgc agacttatca ggtgctgcct
     5581 gctccgggcg cgaccccggc tgaaccgccg ccgccggggg agccaacgct ttaacgttgg
     5641 cgattaacaa aacggcggcg ctgctcgtcg ttcataaagg tccaggcaat aaagcgactc
     5701 tgcttttgcc cctgggccat ctcttttttt accaccttca ccgcgcccac gtcggtcaga
     5761 gcacgataca acggcggtaa gttttcacca cgagatacca gcgaggtaaa ccacatcacc
     5821 tgcttcgcga agcctttgct ctcttcaatc atctttttga taaaggcgac ttcaccacct
     5881 tcacaccaca actcctgttg ctgaccacca aagttcagtg catcgtcttt gttcagcccc
     5941 aggttacggc gtttacgctc actacctgcc cgcgctgcag cggcggaatc gtggaatggc
     6001 gggttacaca aggtcgcgtc gtattgctcg tttttatgga tgatgccgtt aaaaattgcc
     6061 ccactctctt tttgccgacg cagacgaatg gcgcggttaa gccccggatt agcactgata
     6121 atcgcctgcg cactgcttaa cgcctggctg ctggtttcac taccggtaaa acgccagcca
     6181 tattcatgta cgccaatcag cggataaata cagttcgcac caacgccgat atccagaatg
     6241 ctggcattcg ctggaattgt tccgcttgct tcagccagta aatcggcaag gtgatgaata
     6301 taatccgccc ggcccggtac tggtgggcag agaaaaccgt cggggatatc ccagttcgct
     6361 acggcgtaaa aatgggccag caacgcctta ttgagcgcct tcaccgccag cggattggca
     6421 aagtctacgc tttgctcccc ggcgggtgta agcgtgagga attgcctgag ttcaggattg
     6481 acctgacaaa gcgtggcgag atcgtagcgg ctgtgatgac ggttgcgcgg atgcaacccc
     6541 ggtttctggg cggacatagc attctccttt aagcagcggc gtaagatacc cgcataacgc
     6601 gacagggtaa atatctggct gcgtttatca ttccccaaaa ccttaacatg gctatggttt
     6661 atggcaattc atcataaaaa tcagcattat gcatgtcatt gatttgtctt attttgtaaa
     6721 aatatatcgt tcaaaaaata agcacatctc tgtcaatttt ttgcgtcatt ttcatcgccc
     6781 aggcgaccta cacttatcag gcactacccg ttgtatttat gaggatgtca ttatgaaaaa
     6841 gtgcctcaca ctactgattg ccaccgtcct gagcggaatc tctctcacgg cttatgccgc
     6901 gcaaccgatg agtaacctgg acagcggtca gctacggccc gccggtaccg tttcggcaac
     6961 tggcgcatca aacctaagcg atctggagga taaactggca gaaaaagcgc gcgagcaagg
     7021 cgcgaaaggt tatgtcatta attccgcagg cggaaatgac cagatgttcg gtactgcaac
     7081 catctacaaa taaccccacc aacgtaactg cattcgcctc tggatgcagt tttcgcctgt
     7141 tttgtaatca aaaaacatca aatatgaacg caatgtaaat aaatgtattt ctttttcgcg
     7201 caatgggtga tagaaaatcg cgccaagtga taatgcttat caaaattatt atcacttgca
     7261 cgaacactat cacgagatta acagtggcat cgcatccgca gagaggcttt ctcgtggcag
     7321 tgaaaatttc aacatataaa aaaagtcacc tgcaaaatgg aaaacaatcg caatttccct
     7381 gccagacaat ttcattcgct cacgttcttt gccggtcttt gtattggcat cacgcctgtg
     7441 gctcaggcac tcgccgccga agggcaaact aacgcggatg acacgctggt tgtcgaagca
     7501 tcaacgcctt cgctttatgc gccacaacaa tctgccgatc cgaaattctc gcgtccggta
     7561 gcggatacta cccgcacgat gacggtgatt tctgaacaag tgattaaaga tcagggcgca
     7621 accaacctta ccgacgcgct caaaaacgtc cccggcgtgg gtgcgttttt tgcgggtgag
     7681 aacggtaact ccaccactgg cgacgccatt tatatgcgcg gtgctgatac ctctaacagt
     7741 atttatattg atggcattcg cgatatcggc agcgtctcgc gcgacacctt caatactgag
     7801 caggtcgaag tgattaaagg gccttccggc accgactacg ggcgcagcgc gccgacgggc
     7861 tcgatcaata tgatcagcaa gcagccgcgc aatgattccg gcattgacgc ctccgccagt
     7921 attggcagcg cctggttccg ccgcggcacg ctggacgtca atcaggtcat tggtgatacc
     7981 actgcggtgc gcctgaatgt gatgggcgaa aaaacgcacg atgccggacg cgacaaagtc
     8041 aaaaatgagc gttacggcgt cgccccttct atcgcttttg gccttggtac agcgaatcgt
     8101 ttgtatctta attatctgca tgtcacccag cacaacacgc cagacggcgg cattccgacc
     8161 atcggtttgc cgggttattc tgccccatct gcgggaacag cggctctgaa tcattccgga
     8221 aaagttgata ctcataactt ttacggcacg gattccgatt acgacgattc gaccaccgac
     8281 accgccacca tgcgttttga gcacgacatc aacgataaca ccaccattcg caatactacc
     8341 cgttggtcgc gcgtaaagca ggattacctg atgacggcga ttatgggcgg ggcgtcgaat
     8401 attactcagc ccaccagcga tgtgaatagc tggacctggt cacgcacggc gaataccaaa
     8461 gatgtgagta ataaaattct caccaaccag accaacctga cctcgacgtt ctataccggt
     8521 tctatcggtc atgatgtcag taccggcgtg gaatttaccc gtgaaacgca gactaactac
     8581 ggcgttaatc cggtgacgtt acccgcggta aatatttatc atcctgacag cagcattcat
     8641 cccggcggcc tgacgcgcaa cggcgcaaac gccaatggtc agacggatac cttcgcaatt
     8701 tacgccttcg atacgctgca aatcacccgt gattttgagc tgaacggcgg gatccgtctg
     8761 gataattatc atactgaata tgacagtgcc accgcctgcg gcggcagcgg acgcggtgcc
     8821 atcacctgcc caactggtgt ggcaaaaggt tctccggtca ccaccgtcga caccgccaag
     8881 tcgggcaatc tggtgaactg gaaagccggg gcgctgtatc acctgacgga aaacggtaat
     8941 gcctatatta actatgccgt ttcccagcag cctccgggcg gcaacaactt cgcccttgcg
     9001 cagtctggca gcggtaacag tgccaaccgc accgatttta aaccacaaaa agccaacacc
     9061 agcgagattg gcaccaaatg gcaggttctg gataaacgcc tgttgctcac cgccgcgcta
     9121 ttccgtactg atatcgaaaa tgaagttgag caaaatgatg acggaactta ctcgcaatac
     9181 ggtaagaaac gtgtcgaagg ctatgagata tccgtggccg ggaatatcac gcccgcgtgg
     9241 caggtgattg gcggctatac ccagcaaaaa gcaaccatca aaaacggcaa agatgttgcc
     9301 caggatggtt cctcatcgct gccgtatacc ccggagcacg ccttcacctt atggagccag
     9361 tatcaggcaa cagatgatat ctctgttggc gcgggcgcac gctatatcgg cagtatgcat
     9421 aaaggttcag acggcgcggt gggaacgcca gcgtttaccg aaggttactg ggtcgccgat
     9481 gccaaactgg ggtatcgggt taatcgcaat ctcgacttcc agctaaacgt ttacaacctg
     9541 tttgataccg attacgtcgc ctcaatcaac aagagcggct accgttatca cccgggcgag
     9601 ccaagaacct tcttgctcac agccaatatg catttctgat tcagatgtgg ggcgcaggcc
     9661 ccactttttg gagaacttgt atgatgtacc acattcccgg cgtgttatcg ccacaggacg
     9721 tcgcccggtt tcgcgaacaa ctggaacaag ccgaatgggt ggatggacgc gtcaccaccg
     9781 gcgcacaagg tgcgcaagtt aagaacaatc aacaggtcga cacccgcagc acgttatacg
     9841 ccgccctgca aaatgaggtg ctgaacgcgg ttaaccaaca tgctttattc tttgccgcgg
     9901 ccttgccgcg taccctttcc acgccgctgt ttaatcgcta tcagaacaat gaaacctatg
     9961 gtttccatgt ggatggcgca gtacgcagcc atccacaaaa cggctggatg cgtactgacc
    10021 tttctgccac gctgttttta agcgatccac aaagctacga cggcggcgaa ctggtcgtta
    10081 atgacacctt cggacaacat cgggtaaaac tcccggcagg cgatctcgtg ttgtatccct
    10141 ccagcagcct gcattgcgtg acacccgtaa cccgcggcgt acgagtggca tcatttatgt
    10201 ggatccagtc gatgatccgt gatgataaaa agcgcgccat gctgtttgaa ctggacaaca
    10261 atattcagtc gctgaaaagc cgctacggcg aaagtgaaga gatcctgtcg ctgcttaatc
    10321 tttatcataa tctgctgcgg gaatggtcgg agatctgatg cttgaactgc ccaggttcgt
    10381 ccctggatta gctacactta actgtacaag cattgatatg gggaggacga tatggcatcc
    10441 ggttgggcta acgatgacgc cgtcaacgaa cagatcaaca gtacaattga agatgcgatt
    10501 gcccgcgctc ggggtgaaat tccgcgtggc gaaagcctgg atgaatgtga agagtgcggt
    10561 gcccccatcc cgcaagcccg tcgggaagcc attcctggcg tgcgcttatg cattcattgt
    10621 cagcaggaga aaggtttaca aaaaccagcg tatacaggat ataatcgcag aggttcgaaa
    10681 gacagccagt tacgttaact acaactggcg agatgcatag cgagtataat ttgtattttg
    10741 cgtggcattc cgtgccttta acgctgctgt gactgccacg cggcataaaa caagtccata
    10801 agtacaacaa ataaatggtt tatcagtatg ttagatatca atcaatttat ttgaacaagg
    10861 cggtcaattc tcttcgattt tatctctcgt aaaaaaacgt gatactca
//
LOCUS       DAZKHF020000151        10881 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2201S31241
            SAMN43267800-rid25106413.denovo.152, whole genome shotgun sequence.
ACCESSION   DAZKHF020000151 DAZKHF020000000
VERSION     DAZKHF020000151.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267800
            Sequence Read Archive: SRR30307263
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 10881)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10881)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 10881)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350581.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350581.2
            Genome Coverage       :: 151x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:21:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,652
            CDSs (total)                      :: 5,557
            Genes (coding)                    :: 5,320
            CDSs (with protein)               :: 5,320
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 237
            CDSs (without protein)            :: 237
            Pseudo Genes (ambiguous residues) :: 0 of 237
            Pseudo Genes (frameshifted)       :: 102 of 237
            Pseudo Genes (incomplete)         :: 130 of 237
            Pseudo Genes (internal stop)      :: 52 of 237
            Pseudo Genes (multiple problems)  :: 41 of 237
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10881
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267800-rid25106413.denovo.152"
                     /serotype="O 26:H11"
                     /isolate="2201S31241"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-01"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            280..1620
                     /gene="dcuB"
                     /locus_tag="ACHOSC_RS15805"
                     /old_locus_tag="pgaptmp_003161"
     CDS             280..1620
                     /gene="dcuB"
                     /locus_tag="ACHOSC_RS15805"
                     /old_locus_tag="pgaptmp_003161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic C4-dicarboxylate transporter DcuB"
                     /protein_id="HGU4549058.1"
                     /translation="MLFTIQLIIILICLFYGARKGGIALGLLGGIGLVILVFVFHLQP
                     GKPPVDVMLVIIAVVAASATLQASGGLDVMLQIAEKLLRRNPKYVSIVAPFVTCTLTI
                     LCGTGHVVYTILPIIYDVAIKNNIRPERPMAASSIGAQMGIIASPVSVAVVSLVAMLG
                     NVTFDGRHLEFLDLLAITIPSTLIGILAIGIFSWFRGKDLDKDEEFQKFISVPENREY
                     VYGDTATLLDKKLPKSNWLAMWIFLGAIAVVALLGADSDLRPSFGGKPLSMVLVIQMF
                     MLLTGALIIILTKTNPASISKNEVFRSGMIAIVAVYGIAWMAETMFGAHMSEIQGVLG
                     EMVKEYPWAYAIVLLLVSKFVNSQAAALAAIVPVALAIGVDPAYIVASAPACYGYYIL
                     PTYPSDLAAIQFDRSGTTHIGRFVINHSFILPGLIGVSVSCVFGWIFAAMYGFL"
     gene            1698..3344
                     /gene="fumB"
                     /locus_tag="ACHOSC_RS15810"
                     /old_locus_tag="pgaptmp_003162"
     CDS             1698..3344
                     /gene="fumB"
                     /locus_tag="ACHOSC_RS15810"
                     /old_locus_tag="pgaptmp_003162"
                     /EC_number="4.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I fumarate hydratase"
                     /protein_id="HGU4549059.1"
                     /translation="MSNKPFIYQAPFPMGKDNTEYYLLTSDYVSVADFDGETILKVEP
                     EALTLLAQQAFHDASFMLRPAHQKQVAAILHDPEASENDKYVALQFLRNSEIAAKGVL
                     PTCQDTGTAIIVGKKGQRVWTGGGDEEALSKGVYNTYIEDNLRYSQNAALDMYKEVNT
                     GTNLPAQIDLYAVDGDEYKFLCVAKGGGSANKTYLYQETKALLTPGKLKNFLVEKMRT
                     LGTAACPPYHIAFVIGGTSAETNLKTVKLASAHYYDELPTEGNEHGQAFRDVQLEQEL
                     LEEAQKLGLGAQFGGKYFAHDIRVIRLPRHGASCPVGMGVSCSADRNIKAKINREGIW
                     IEKLEHNPGQYIPQELRQAGEGEAVKVDLNRPMKEILAQLSQYPVSTRLSLTGTIIVG
                     RDIAHAKLKELIDAGKELPQYIKDHPIYYAGPAKTPAGYPSGSLGPTTAGRMDSYVDL
                     LQSHGGSMIMLAKGNRSQQVTDACHKHGGFYLGSIGGPAAVLAQQSIKHLECVAYPEL
                     GMEAIWKIEVEDFPAFILVDDKGNDFFQQIVNKQCANCTK"
     gene            3467..4096
                     /gene="yjdF"
                     /locus_tag="ACHOSC_RS15815"
                     /old_locus_tag="pgaptmp_003163"
     CDS             3467..4096
                     /gene="yjdF"
                     /locus_tag="ACHOSC_RS15815"
                     /old_locus_tag="pgaptmp_003163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2238 domain-containing protein"
                     /protein_id="HGU4549060.1"
                     /translation="MTRTLKPLILNTGALALTLILIYTGISAHDKLTWLMEVTPVIIV
                     VPLLLATAKRYPLTPLLYTLIFFHAIILMVGGQYTYAKVPIGFEVQEWLGLSRNPYDK
                     LGHFFQGLVPALVAREILVRGMYVRGRKMVAFLVCCVALAISAMYELIEWWAALAMGQ
                     GADDFLGTQGDQWDTQSDMFCALLGALTTVIFLARFHCRQLRRFGLITG"
     gene            complement(4235..5656)
                     /gene="melB"
                     /locus_tag="ACHOSC_RS15820"
                     /old_locus_tag="pgaptmp_003164"
     CDS             complement(4235..5656)
                     /gene="melB"
                     /locus_tag="ACHOSC_RS15820"
                     /old_locus_tag="pgaptmp_003164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418544.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015293 - symporter activity [Evidence
                     IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="melibiose:sodium transporter MelB"
                     /protein_id="HGU4549061.1"
                     /translation="MSISMTTKLSYGFGAFGKDFAIGIVYMYLMYYYTDVVGLSVGLV
                     GTLFLVARIWDAINDPIMGWIVNATRSRWGKFKPWILIGTLANSVILFLLFSAHLFEG
                     TTQIVFVCVTYILWGMTYTIMDIPFWSLVPTITLDKREREQLVPYPRFFASLAGFVTA
                     GVTLPFVNYVGGGDRGFGFQMFTLVLIAFFIVSTIITLRNVHEVFSSDNQPSAEGSHL
                     TLKAIVALIYKNDQLSCLLGMALAYNVASNIITGFAIYYFSYVIGDADLFPYYLSYAG
                     AANLVTLVFFPRLVKSLSRRILWAGASILPVLSCGVLLLMALMSYHNVVLIVIAGILL
                     NVGTALFWVLQVIMVADTVDYGEYKLHVRCESIAYSVQTMVVKGGSAFAAFFIAVVLG
                     MIGYVPNVEQSTQALLGMQFIMIALPTLFFMVTLILYFRFYRLNGDTLRRIQIHLLDK
                     YRKVPPEPVHADIPVGAVSDVKA"
     gene            complement(5759..7114)
                     /gene="melA"
                     /locus_tag="ACHOSC_RS15825"
                     /old_locus_tag="pgaptmp_003165"
     CDS             complement(5759..7114)
                     /gene="melA"
                     /locus_tag="ACHOSC_RS15825"
                     /old_locus_tag="pgaptmp_003165"
                     /EC_number="3.2.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-galactosidase"
                     /protein_id="HGU4549062.1"
                     /translation="MMTAPKITFIGAGSTIFVKNILGDVFHREALKTAHIALMDIDPT
                     RLEESHIVVRKLMDSAGASGKITCHTQQKEALHDADFVVVAFQIGGYEPCTVTDFEVC
                     KRHGLEQTIADTLGPGGIMRALRTIPHLWQICEDMTEVCPDATMLNYVNPMAMNTWAM
                     YARYPHIKQVGLCHSVQGTAEELARDLNIDPATLRYRCAGINHMAFYLELERKTADGS
                     YVNLYPELLAAYEAGQAPKPNIHGNTRCQNIVRYEMFKKLGYFVTESSEHFAEYTPWF
                     IKPGREDLIERYKVPLDEYPKRCVEQLANWHKELEEYKNASRIDIKPSREYASTIMNA
                     IWTGEPSVIYGNVRNDGLIDNLPQGCCVEVACLVDANGIQPTKVGTLPSHLAALMQTN
                     INVQTLLTEAILTENRDRVYHAAMMDPHTAAVLGIDEIYALVDDLIAAHGDWLPGWLH
                     R"
     gene            7397..8305
                     /gene="melR"
                     /locus_tag="ACHOSC_RS15830"
                     /old_locus_tag="pgaptmp_003166"
     CDS             7397..8305
                     /gene="melR"
                     /locus_tag="ACHOSC_RS15830"
                     /old_locus_tag="pgaptmp_003166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator MelR"
                     /protein_id="HGU4549063.1"
                     /translation="MNTDTFMCSSDEKQTRSPLSLYSEYQRMEIEFRAPHIMPTSHWH
                     GQVEVNVPFDGDVEYLINNEKVNINQGHITLFWACTPHQLTDTGTCKSMAIFNLPMHL
                     FLSWPLDKDLINHVTHGMVIKSLATQQLSPFEVRRWQQELNSPNEQIRQLAIDEIGLM
                     LKRFSLSGWEPILVNKTSRTHKNSVSRHAQFYVSQMLGFIAENYDQALTINDVAEHVK
                     LNANYAMGIFQRVMQLTMKQYITAMRINHVRALLSDTDKSILDIALTAGFRSSSRFYS
                     TFGKYVGMSPQQYRKLRQQRRQTFPG"
     gene            8504..10771
                     /gene="adiA"
                     /locus_tag="ACHOSC_RS15835"
                     /old_locus_tag="pgaptmp_003167"
     CDS             8504..10771
                     /gene="adiA"
                     /locus_tag="ACHOSC_RS15835"
                     /old_locus_tag="pgaptmp_003167"
                     /EC_number="4.1.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418541.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016831 - carboxy-lyase activity [Evidence
                     IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine decarboxylase"
                     /protein_id="HGU4549064.1"
                     /translation="MKVLIVESEFLHQDTWVGNAVERLADALSQQNVTVIKSTSFDDG
                     FAILSSNEAIDCLMFSYQMEHPDEHQNVRQLIGKLHERQQNVPVFLLGDREKALAAMD
                     RDLLELVDEFAWILEDTADFIAGRAVAAMTRYRQQLLPPLFSALMKYSDIHEYSWAAP
                     GHQGGVGFTKTPAGRFYHDYYGENLFRTDMGIERTSLGSLLDHTGAFGESEKYAARVF
                     GADRSWSVVVGTSGSNRTIMQACMTDNDVVVVDRNCHKSIEQGLMLTGAKPVYMVPSR
                     NRYGIIGPIYPQEMQPETLQKKISESPLTKDKAGQKPSYCVVTNCTYDGVCYNAKEAQ
                     DLLEKTSDRLHFDEAWYGYARFNPIYADHYAMRGEPGDHNGPTVFATHSTHKLLNALS
                     QASYIHVREGRGAINFSRFNQAYMMHATTSPLYAICASNDVAVSMMDGNSGLSLTQEV
                     IDEAVDFRQAMARLYKEFTADGSWFFKPWNKEVVTDPQTGKTYDFADAPTKLLTTVQD
                     CWVMHPGESWHGFKDIPDNWSMLDPIKVSILAPGMGEDGELEETGVPAALVTAWLGRH
                     GIVPTRTTDFQIMFLFSMGVTRGKWGTLVNTLCSFKRHYDANTPLAQVMPELVEQYPD
                     TYANMGIHDLGDTMFAWLKENNPGARLNEAYSGLPMAEITPREAYNAIVDNNVELVSI
                     ENLPGRIAANSVIPYPPGIPMLLSGENFGDKNSPQVSYLRSLQSWDHHFPGFEHETEG
                     TEIIDGIYHVMCVKA"
BASE COUNT         2791 a         2879 c         2666 g         2545 t
ORIGIN      
        1 taaatatcaa taagataaca gcaaacaaaa cattaacatc tgcgcagtac aaactataaa
       61 cccatcgcca gagagttttt ctctctgaaa aagccgctta tcacagtgca taaatttgcc
      121 gctgctttaa tcagccaata tttactgtga ggtatttgct aaagccggta acgaccaaac
      181 ggatatttag tcaggctctg aaaacagttc atacaaaaca gaacgtgact gtgatctatt
      241 cagcaaaaat ttaaatagga ttatcgcgag ggttcacaca tgttatttac tatccaactt
      301 atcataatac tgatatgtct gttttatggt gccagaaagg gtggtatcgc gctgggttta
      361 ttaggcggta tcggtctggt cattctggtc ttcgtcttcc accttcagcc aggtaaacca
      421 ccggttgatg tcatgctggt tatcattgcg gtggtggcgg catcggcgac cttgcaagct
      481 tcgggcggtc ttgatgtcat gctgcaaatt gccgagaagc tgctgcgccg caacccgaaa
      541 tatgtctcaa ttgtcgcgcc gtttgtgacc tgtacactga ccattctttg cggtacgggt
      601 catgtggttt acaccattct gccgatcatc tacgacgtcg ccattaagaa caacatccgt
      661 ccggaacgtc cgatggcggc aagttctatc ggtgcacaga tggggattat cgccagtccg
      721 gtgtcggttg cggtcgtgtc tctggttgca atgctgggta atgtcacctt tgatggtcgc
      781 catcttgagt tcctcgacct gctggcaatc accattccat cgacgttaat cggtatcctg
      841 gcgatcggta tcttcagctg gttccgcggt aaagatctgg ataaagacga agagttccag
      901 aaattcatct ccgtaccgga aaaccgtgag tatgtttacg gtgataccgc gacgctgctc
      961 gataaaaaac tgccgaaaag caactggctg gcaatgtgga ttttcctcgg tgcaatcgct
     1021 gtagtcgccc ttcttggtgc tgattcggac ctgcgtccat ccttcggcgg caaaccgctg
     1081 tcgatggtac tggttattca gatgtttatg ctgctgaccg gggcgctgat tattatcctg
     1141 accaaaacca atcccgcgtc tatctcaaaa aacgaagtct tccgttccgg tatgatcgcc
     1201 atcgtggcgg tgtacggtat cgcatggatg gcagaaacca tgttcggtgc gcatatgtct
     1261 gaaattcagg gcgtactggg tgaaatggtg aaagagtatc cgtgggcgta tgccattgtt
     1321 ctgctgctgg tttccaagtt tgtaaactct caggctgcgg cgctggcggc gattgttccg
     1381 gtcgcgctgg cgatcggcgt tgatccggca tacatcgtgg cttcagcacc ggcttgctac
     1441 ggttattaca tcctgccgac ttatccgagc gatctggcag cgattcagtt tgaccgttcc
     1501 ggcaccaccc acatcggtcg cttcgtcatc aaccacagtt ttattctgcc ggggttgatt
     1561 ggtgtgagcg tatcgtgcgt cttcggctgg atcttcgccg cgatgtacgg gttcttataa
     1621 atgcactttg cgtgccgccc gtgactacgc ggcacgccat tttcgaataa caaatacaga
     1681 gttacaggct ggaagctatg tcaaacaaac cctttatcta ccaggcacct ttcccgatgg
     1741 ggaaagacaa taccgaatac tatctactca cttccgatta cgttagcgtt gccgacttcg
     1801 acggcgaaac catcctgaaa gtggaaccag aagccctgac cctactggcg cagcaagcct
     1861 ttcacgacgc ttctttcatg ctccgtccgg cacaccagaa acaggttgcg gctattcttc
     1921 acgatccaga agccagcgaa aacgacaagt acgtggcgct gcaattctta agaaactccg
     1981 aaatcgccgc caaaggcgtg ctgccgacct gccaggatac cggcaccgcg atcatcgtcg
     2041 gtaaaaaagg ccagcgcgtg tggaccggtg gcggtgatga agaagcgctg tcgaaaggcg
     2101 tctataacac ctatatcgaa gataacctgc gctattcaca gaatgcggcg ctggacatgt
     2161 acaaagaggt caacaccggc actaacctgc ctgcgcaaat cgacctgtac gcggtagatg
     2221 gcgatgagta caaattcctt tgcgttgcga aaggcggcgg ctctgccaac aaaacgtatc
     2281 tctaccagga aaccaaagcc ctgctgactc ccggcaaact gaaaaacttc ctcgtcgaga
     2341 aaatgcgtac cctcggtact gcagcctgcc cgccgtacca tatcgcgttt gtgattggcg
     2401 gtacgtctgc ggaaaccaac ctgaaaaccg tcaagttagc aagcgctcac tattacgatg
     2461 aactgccgac ggaagggaac gaacatggtc aggcgttccg cgatgtccag ctggaacagg
     2521 aactgctgga agaggcccag aaactcggtc ttggcgcgca gtttggcggt aaatacttcg
     2581 cgcacgacat tcgcgttatc cgtctgccac gtcacggcgc atcctgcccg gtcggcatgg
     2641 gcgtctcctg ctccgctgac cgtaacatta aagcgaaaat caaccgcgaa ggtatctgga
     2701 tcgaaaaact ggaacacaac ccaggccagt acattccaca agaactgcgc caggccggtg
     2761 aaggcgaagc ggtgaaagtt gaccttaacc gcccgatgaa agagatcctc gcccagcttt
     2821 cgcaataccc ggtatccact cgtttgtcgc tcaccggcac cattatcgtg ggccgagata
     2881 ttgcacacgc caagctgaaa gagctgattg acgccggtaa agaacttccg cagtacatca
     2941 aagatcaccc gatctactac gcgggtccgg cgaaaacccc tgccggttat ccatcaggtt
     3001 cacttggccc aaccaccgca ggccgtatgg actcctacgt ggatctgctg caatcccacg
     3061 gcggcagcat gatcatgctg gcgaaaggta accgcagtca gcaggttacc gacgcgtgtc
     3121 ataaacacgg cggcttctac ctcggtagca tcggcggtcc ggcggcggta ctggcgcagc
     3181 agagcatcaa gcatctggag tgcgtcgctt atccggagct gggtatggaa gctatctgga
     3241 aaatcgaagt agaagatttc ccggcgttta tcctggtcga tgacaaaggt aacgacttct
     3301 tccagcaaat cgtcaacaaa cagtgcgcga actgcactaa gtaacctctt cggcccagcg
     3361 cctggcagca tgctgccagg tgatccccct gggccacctc ttttgcgatt gtaatttcac
     3421 gcttgctggt gaatagtcag tattttccct gacttgcgaa ctcaccatga cccgtacact
     3481 caagccgtta attcttaaca ccggcgcact ggcgctaacg ttaatcctga tttataccgg
     3541 catttcggcc catgacaaac tcacctggct gatggaagtg acaccggtga ttattgtcgt
     3601 gccgctactg cttgccaccg ctaaacgtta tccgttaacg ccgctgctct atacgctcat
     3661 tttctttcac gccatcatcc tgatggtcgg cgggcaatac acctacgcga aagtccccat
     3721 cggttttgag gtgcaggaat ggctggggtt gagccgtaat ccgtatgaca agctggggca
     3781 ttttttccag gggctggtgc ctgcactggt ggcacgagaa attctcgtgc gcgggatgta
     3841 cgtgcgcgga cgtaaaatgg tggcgttcct ggtctgctgc gtggcgctgg cgataagcgc
     3901 gatgtatgaa ttaatcgagt ggtgggcggc gctggcaatg ggccagggag cggatgattt
     3961 tctcggcacc cagggcgacc agtgggacac gcaatccgat atgttctgcg cgctgcttgg
     4021 cgcattaacg acggtgatat tcctcgctcg ctttcattgc cgccagctac ggcgctttgg
     4081 cttgataacc ggataacaac gtgcgctttg ttgatgccgg atgcggcgtg aattccttat
     4141 ccggtctaca aaatttgcaa atccaaagca ttacattaag acgtggcctg ataaacatat
     4201 cgcatcaggc cattttcgtt agctattgtc cacgtcaggc tttcacatca ctcactgcac
     4261 cgaccggaat atcagcatga acaggctcgg gcggtacttt gcgatattta tccagcagat
     4321 gaatctggat cctgcgcagc gtgtcgccat tgaggcgata gaaacggaag tagagaatca
     4381 gcgttaccat gaaaaacaga gttggtagag caatcataat aaactgcata cctaataggg
     4441 cttgcgtaga ctgttcaaca ttcggtacat agccaatcat ccctaacaca accgcaatga
     4501 aaaaagccgc aaaggctgaa ccgcccttca ccaccatagt ctgcacggag taagcgatac
     4561 tttcacagcg tacgtgcagt ttatattcac cgtaatcaac ggtatctgcc accatgatga
     4621 cctgtaatac ccagaaaagc gccgttccca cattcagcaa aatacccgca atcacaatga
     4681 ggacgacgtt gtgatagctc attaatgcca ttaacaggag aacaccacag cttaacaccg
     4741 gaagaataga tgctccggcc cataaaatgc gtcgggataa tgatttaact aagcgtggga
     4801 agaatactaa cgtcaccagg ttagcagctc ccgcatacga cagataatag gggaacaaat
     4861 ccgcatcacc gataacatat gagaaataat agatagcaaa tccggtaata atgttgctgg
     4921 ctacattata agcaagagcc atacccaaga ggcatgaaag ctgatcgttt ttataaatta
     4981 gcgcaacgat ggctttaagt gtcagatggc ttccttcagc agacggttga ttgtctgacg
     5041 aaaagacttc atgcacattg cgcagagtga tgatggttga aacaataaaa aaggcgatta
     5101 gtaccagagt gaacatctga aagccaaatc cccgatcgcc accgccgaca taattaacaa
     5161 atggtagcgt cacacctgcc gtaacaaagc ctgccagact ggcaaaaaaa cgcggataag
     5221 gaaccagttg ttcgcgctca cgtttatcga gcgtgatggt tggaaccagc gaccagaagg
     5281 gaatatccat aatggtgtaa gtcatgcccc agaggatgta ggtcacgcaa acaaagacaa
     5341 tctgagtagt accttcaaac agatgcgcac taaagaggag aaataagatt acagagtttg
     5401 ccaacgtacc gatcaggatc cagggtttga acttacccca tcgcgatcgc gtagcattta
     5461 caatccatcc cataatcgga tcgttaatag catcccagat cctcgccacc agaaacaaag
     5521 taccgaccaa acccacagac agcccaacga catcggtgta gtaatacatg aggtacatat
     5581 acacaatgcc gatcgcaaaa tccttcccga acgctccaaa tccataactg agttttgtag
     5641 tcattgaaat gctcataggg tatcgggtcg ctgttaccaa cgcccgcctt ctgttattgg
     5701 tatcgtcaga ggtgtgcgcc ggaataaatc ccccggcgca gtagcgttta gtcgcgtttt
     5761 aacggtgcaa ccagcctggc agccagtcgc cgtgggcggc aatcaggtcg tcaacaagag
     5821 catatatttc atcaatgccc agcacggcag cagtatgcgg gtccatcatc gcggcgtggt
     5881 aaacacggtc gcgattttcc gtaagaatag cttcggtcag cagcgtctgt acgttgatgt
     5941 tggtttgcat cagggcggcc agatgcgaag gtagcgtacc gactttggtc ggctgaatgc
     6001 cattagcatc aaccagacag gctacttcca cgcaacatcc ttgtggcagg ttatcaatca
     6061 aaccatcgtt acggacgttg ccgtaaatca cactcggctc gccagtccag atagcgttca
     6121 tgattgtgct ggcatattcc cgtgacggtt taatatcaat ccgggaggcg tttttatact
     6181 cctccagctc tttatgccag ttcgccagct gctcgacgca gcgtttcggg tactcatcca
     6241 gcggtacttt ataacgctca atcaaatcct cacgacctgg cttaataaac cacggtgtgt
     6301 actcagcaaa atgttctgac gattccgtga cgaagtagcc cagctttttg aacatttcgt
     6361 agcgcacaat attctggcag cgagtattgc catgaatatt cggcttcggt gcctgccctg
     6421 cttcataagc cgccagcagt tccgggtaga gattcacata actgccgtcg gcggttttgc
     6481 gctccagctc caggtaaaac gccatatggt tgatacctgc gcaacggtaa cgcagcgtag
     6541 ctgggtcgat attgaggtca cgcgccagct cttccgccgt tccctgcacc gaatggcaca
     6601 gcccgacctg tttgatatgc ggatagcggg catacatcgc ccaggtattc atcgccattg
     6661 ggttaacata gttgagcatg gtggcatcgg ggcagacttc cgtcatgtcc tcgcaaattt
     6721 gccacagatg cggaatggta cgtagcgcgc gcataatacc gcccggcccc aacgtatcgg
     6781 caatggtttg ttccagacca tgccgcttac agacctcgaa atcagtcacc gtgcaaggtt
     6841 cataaccgcc aatctgaaat gccaccacga caaaatcggc atcatgtaag gcttctttct
     6901 gttgggtgtg gcaggtgatt ttgccgctgg cccctgctga atccataagc ttacgcacca
     6961 caatatgcga ctcttccagg cgggtgggat caatgtccat cagggcaata tgcgccgttt
     7021 tcagcgcctc gcgatggaac acatcaccaa gaatattttt aacgaaaatc gtcgaaccag
     7081 cgccgataaa tgtaattttg ggtgcagtca tcatgcagat ctcctggctt gcttgaataa
     7141 cttcatcatg gcaggcgaat ctgcagaaaa cctccggctt ccaggttgag cattcccata
     7201 aactcagatc ctgcgtgaag cagcagtaaa tctgagttta tggtaataag ttacctgaaa
     7261 agcagagttt tcggcctcat gatgctcgga cgctcccctc gcctctctgg gaagagggtg
     7321 aaaaccgtgc tcccactcgc agtcatcctc cctcactcct gccataattc tgatattcca
     7381 ggaaagagag ccatccatga atacagatac gtttatgtgc agcagcgacg aaaaacagac
     7441 ccgcagcccg ctgtcgctgt attcggaata ccagcggatg gagattgagt ttcgcgcacc
     7501 gcatatcatg cccaccagcc actggcatgg tcaggtcgaa gtgaatgtgc ctttcgatgg
     7561 cgatgtggaa tacctgatca acaatgaaaa agtgaatatc aatcagggtc atatcacgct
     7621 gttctgggcc tgtacaccgc accaactaac agataccgga acctgtaaga gcatggcgat
     7681 ttttaatctg ccgatgcatc tgtttctctc ctggccgctg gataaagacc tgattaacca
     7741 cgtcactcac ggcatggtga tcaaatcact ggccacacag caacttagcc cgtttgaagt
     7801 gcgccgctgg cagcaggaat taaacagtcc gaacgagcaa attcgccagc tcgccattga
     7861 tgaaattggc ctgatgctca agcgatttag cctctctggc tgggaaccga ttctggtcaa
     7921 taaaacctcg cgcacacaca aaaacagcgt ctcgcgccat gcgcaatttt atgttagcca
     7981 gatgctgggc tttattgccg aaaactatga tcaggcgctg accatcaacg atgtggctga
     8041 gcacgtcaaa cttaacgcca actatgcaat ggggatattc cagcgggtca tgcaattgac
     8101 gatgaaacag tacattaccg cgatgcgcat caaccacgtt cgcgcgttac tgagcgatac
     8161 cgataaaagt attctcgata ttgccctgac ggcaggcttt cgttcaagta gtcgttttta
     8221 cagcacgttc ggcaaatatg tcggcatgtc gccgcaacaa taccgcaaac ttcgccaaca
     8281 gcgccgccag acgtttcccg gctaaagacg atatcagtat cagccaaaaa aatagtttag
     8341 ccgtcgataa taagcagtga aaataattta aaataatcac ataacgtatt gtttataaaa
     8401 ctaaatattt caaaaatgtt tgtttttcac gcgctttaca gcccgaaaag gccggaagat
     8461 acttgcccgc aacgaagatt ccttcataac cgggtaagca atgatgaaag tattaattgt
     8521 tgaaagcgag tttctccatc aagacacctg ggtcggtaac gccgttgagc gtctggcaga
     8581 tgctttaagc cagcaaaatg ttaccgtgat taaatccacc tcctttgatg atggttttgc
     8641 cattctctct tcaaacgaag ccattgactg cctgatgttc agctatcaaa tggaacatcc
     8701 ggacgaacat caaaacgtca gacaattgat cggtaagctt catgagcgcc aacaaaacgt
     8761 gccggtcttc ctgttgggcg atcgggaaaa agccctcgcc gcaatggacc gggatctgct
     8821 ggagcttgtc gatgaattcg cctggattct ggaagatacc gccgacttta tcgccggacg
     8881 cgccgttgcc gcgatgaccc gctaccgcca gcagctgttg ccgccactgt tcagcgcgct
     8941 gatgaaatat agtgacatcc atgaatattc ctgggcagcg ccaggccacc agggcggcgt
     9001 tggttttacc aaaacacccg ccggacgttt ctaccatgac tactatggtg aaaatctgtt
     9061 ccgcaccgac atgggcatcg aacgaacttc cctcggttct ttgcttgacc atactggcgc
     9121 atttggcgaa agcgaaaaat atgccgcacg cgtatttggt gccgatcgct cctggtcggt
     9181 agtcgtcggt acttccggct ctaaccgcac catcatgcag gcttgcatga ccgataacga
     9241 tgtcgtggtc gttgaccgta attgccataa atccatcgaa caaggcttga tgctgacagg
     9301 cgcgaaaccg gtctatatgg tgccaagccg caaccgctac ggcattatcg ggccaatcta
     9361 tccgcaggaa atgcaacctg aaaccttgca gaagaaaatc agtgaaagcc cgctgaccaa
     9421 agacaaagcc gggcaaaaac cgtcttactg cgtggtgacc aactgcacct atgacggcgt
     9481 gtgttataac gctaaagaag cgcaggatct gctggaaaaa acctccgatc gtctgcactt
     9541 tgacgaagcc tggtacggct atgcacgttt caacccgatc tatgccgatc actatgccat
     9601 gcgcggcgaa cctggcgatc acaacggtcc taccgttttc gccacccact ccacccacaa
     9661 actgctgaat gcgctgtcac aggcttctta tattcatgta cgtgaaggtc gtggggcgat
     9721 taacttctcc cgcttcaacc aggcctacat gatgcatgcc accacctccc cgctgtatgc
     9781 catctgcgca tccaacgacg tggcggtgtc gatgatggac ggcaacagcg gcctgtcact
     9841 gacacaggaa gtgattgacg aagcggttga tttccgtcag gcgatggcgc ggctatataa
     9901 agagttcacc gctgacggta gctggttctt caaaccgtgg aacaaagaag tcgtcaccga
     9961 cccacaaacc ggcaaaacct atgactttgc tgacgcacca accaaactgc tgaccaccgt
    10021 tcaggactgc tgggtaatgc atccgggcga aagctggcac ggcttcaaag atattccgga
    10081 taactggagt atgctcgacc caattaaagt cagcatcctt gctccgggaa tgggtgaaga
    10141 tggtgaactg gaagaaaccg gtgttccggc ggcgctggtc actgcctggc ttggtcgcca
    10201 cggcattgtg cctacccgca ccactgactt ccaaattatg ttcctgttct ctatgggcgt
    10261 aacccgtggg aaatggggga ctctggttaa caccctttgt tccttcaaac gccactatga
    10321 cgccaacaca ccgctggcgc aggtgatgcc ggaacttgtt gaacaatatc ctgacactta
    10381 cgcgaacatg gggattcacg atctgggtga caccatgttt gcctggctga aagaaaacaa
    10441 ccctggcgca cggttgaacg aagcctattc cggcctgccg atggcggaaa tcaccccgcg
    10501 tgaagcgtac aacgcgattg tcgacaacaa tgtcgaactg gtatccattg aaaatctgcc
    10561 aggacgcatc gcggcaaact cagttatccc gtatccgcca ggaatcccga tgctgctgtc
    10621 tggtgaaaac ttcggcgata aaaacagtcc gcaagtaagt tatttacgct cgctgcaatc
    10681 ctgggaccac catttccctg gatttgaaca cgaaactgaa gggactgaaa ttattgacgg
    10741 tatttaccac gttatgtgcg tgaaagcgta accactattc cgctgaaggc gtaattgttt
    10801 aaataacatt acgccgcctg gccttaggcc ttttgagtat ggcaacgttt tcataaaaat
    10861 tgctgcaaac aaaaatgtca t
//
LOCUS       DAZKHF020000152        10865 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2201S31241
            SAMN43267800-rid25106413.denovo.153, whole genome shotgun sequence.
ACCESSION   DAZKHF020000152 DAZKHF020000000
VERSION     DAZKHF020000152.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267800
            Sequence Read Archive: SRR30307263
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 10865)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10865)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 10865)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350581.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350581.2
            Genome Coverage       :: 151x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:21:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,652
            CDSs (total)                      :: 5,557
            Genes (coding)                    :: 5,320
            CDSs (with protein)               :: 5,320
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 237
            CDSs (without protein)            :: 237
            Pseudo Genes (ambiguous residues) :: 0 of 237
            Pseudo Genes (frameshifted)       :: 102 of 237
            Pseudo Genes (incomplete)         :: 130 of 237
            Pseudo Genes (internal stop)      :: 52 of 237
            Pseudo Genes (multiple problems)  :: 41 of 237
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10865
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267800-rid25106413.denovo.153"
                     /serotype="O 26:H11"
                     /isolate="2201S31241"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-01"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            92..853
                     /gene="adiY"
                     /locus_tag="ACHOSC_RS15840"
                     /old_locus_tag="pgaptmp_003168"
     CDS             92..853
                     /gene="adiY"
                     /locus_tag="ACHOSC_RS15840"
                     /old_locus_tag="pgaptmp_003168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional activator AdiY"
                     /protein_id="HGU4549065.1"
                     /translation="MRICSDQPCIVLLTEKDVWIRVNGKEPISLKANHMALLNCENNI
                     IDVSSLNNTLVAHISHDIIKDYLRFLNKDLSQIPVWQRSATPILTLPCLTPDVFRVAA
                     QHSMMPAETESEKERTRALLFTVLSRFLDSKKFLSLMMYMLRNCVSDSVYQIIESDIH
                     KDWNLSMVASCLCLSPSLLKKKLKSENTSYSQIITTCRMRYAVNELMMDGKNISQVSQ
                     SCGYNSTSYFISVFKDFYGMTPLHYVSQHRERTVA"
     gene            990..2327
                     /gene="adiC"
                     /locus_tag="ACHOSC_RS15845"
                     /old_locus_tag="pgaptmp_003169"
     CDS             990..2327
                     /gene="adiC"
                     /locus_tag="ACHOSC_RS15845"
                     /old_locus_tag="pgaptmp_003169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine/agmatine antiporter"
                     /protein_id="HGU4549066.1"
                     /translation="MSSDADAHKVGLIPVTLMVSGNIMGSGVFLLPANLASTGGIAIY
                     GWLVTIIGALGLSMVYAKMSFLDPSPGGSYAYARRCFGPFLGYQTNVLYWLACWIGNI
                     AMVVIGVGYLSYFFPILKDPLVLTITCVVVLWIFVLLNIVGPKMITRVQAVATVLALI
                     PIVGIAVFGWFWFRGETYMAAWNVSGLGTFGAIQSTLNVTLWSFIGVESASVAAGVVK
                     NPKRNVPIATIGGVLIAAVCYVLSTTAIMGMIPNAALRVSASPFGDAARMALGDTAGA
                     IVSFCAAAGCLGSLGGWTLLAGQTAKAAADDGLFPPIFARVNKAGTPVAGLIIVGILM
                     TIFQLSSISPNATKEFGLVSSVSVIFTLVPYLYTCAALLLLGHGHFGKARPAYLAVTT
                     IAFLYCIWAVVGSGAKEVMWSFVTLMVITAMYALNYNRLHKNPYPLDVPISKD"
     gene            2431..4074
                     /gene="eptA"
                     /locus_tag="ACHOSC_RS15850"
                     /old_locus_tag="pgaptmp_003170"
     CDS             2431..4074
                     /gene="eptA"
                     /locus_tag="ACHOSC_RS15850"
                     /old_locus_tag="pgaptmp_003170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418538.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016772 - transferase activity,
                     transferring phosphorus-containing groups [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoethanolamine transferase EptA"
                     /protein_id="HGU4549067.1"
                     /translation="MLKRLLKRPSLNLLAWLLLAAFYISICLNIAFFKQVLQALPLDS
                     LHNVLVFLSMPVVAFSVINIVLTLSSFLWLNRPLACLFILVGAAAQYFIMTYGIVIDR
                     SMIANIIDTTPAESYALMTPQMLLTLGFSGVLAALIACWIKIKPATSRLRSVLFRGAN
                     ILVSVLLILLVAALFYKDYASLFRNNKELVKSLSPSNSIVASWSWYSHQRLANLPLVR
                     IGEDAHRNPLMQNEKRKNLTILIVGETSRAENFSLNGYPRETNPRLAKDNVVYFPNTA
                     SCGTATAVSVPCMFSDMPREHYKEELAQHQEGVLDIIQRAGINVLWNDNDGGCKGACD
                     RVPHQNVTALNLPGQCINGECYDEVLFHGLEEYINNLQGDGVIVLHTIGSHGPTYYNR
                     YPPQFRKFTPTCDTNEIQTCSKEQLVNTYDNTLVYVDYIVDKAINLLKEHQDKFTTSL
                     VYLSDHGESLGENGIYLHGLPYAIAPDSQKQVPMLLWLSEDYQKRYQVDQNCLQKQAQ
                     TQHYSQDNLFSTLLGLTGVETKYYQAADDILQTCRRVSE"
     gene            4071..4739
                     /gene="basR"
                     /locus_tag="ACHOSC_RS15855"
                     /old_locus_tag="pgaptmp_003171"
     CDS             4071..4739
                     /gene="basR"
                     /locus_tag="ACHOSC_RS15855"
                     /old_locus_tag="pgaptmp_003171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator BasR"
                     /protein_id="HGU4549068.1"
                     /translation="MKILIVEDDTLLLQGLILAAQTEGYACDGVTTARMAEQSLEAGH
                     YSLVVLDLGLPDEDGLHFLARIRQKKYTLPVLILTARDTLTDKIAGLDVGADDYLVKP
                     FALEELHARIRALLRRHNNQGESELIVGNLTLNMGRRQVWMGGEELILTPKEYALLSR
                     LMLKAGSPVHREILYNDIYNWDNEPSTNTLEVHIHNLRDKVGKARIRTVRGFGYMLVA
                     NEEN"
     gene            4740..5840
                     /gene="pmrB"
                     /locus_tag="ACHOSC_RS15860"
                     /old_locus_tag="pgaptmp_003172"
     CDS             4740..5840
                     /gene="pmrB"
                     /locus_tag="ACHOSC_RS15860"
                     /old_locus_tag="pgaptmp_003172"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     PmrB"
                     /protein_id="HGU4549069.1"
                     /translation="MNLMHFLRRPISLRQRLILTIGAILLVFELISVFWLWHESTEQI
                     QLFEQALRDNRNNDRHIMREIREAVASLIVPGVFMVSLTLFICYQAVRRITRPLAELQ
                     KELEARTADNLTPIAIHSATLEIEAVVSALNDLVSRLTSTLDNERLFTADVAHELRTP
                     LAGVRLHLELLAKTHHIDVAPLVARLDQMMESVSQLLQLARAGQSFSSGNYQHVKLLE
                     DVILPSYDELSTMLDQRQQTLLLPESAADITVQGDATLLRMLLRNLVENAHRYSPQGS
                     NIMIKLQEDGGAVMAVEDEGPGIDESKCGELSKAFVRMDSRYGGIGLGLSIVSRITQL
                     HHGQFFLQNRQETSGTRAWVRLKKDQNVANQI"
     gene            complement(5816..5905)
                     /gene="pmrR"
                     /locus_tag="ACHOSC_RS15865"
                     /old_locus_tag="pgaptmp_003173"
     CDS             complement(5816..5905)
                     /gene="pmrR"
                     /locus_tag="ACHOSC_RS15865"
                     /old_locus_tag="pgaptmp_003173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000797656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LpxT activity modulator PmrR"
                     /protein_id="HGU4549070.1"
                     /translation="MKNRVYESLTTVFSVLVVSSFLYIWFATF"
     gene            complement(6017..7519)
                     /gene="proP"
                     /locus_tag="ACHOSC_RS15870"
                     /old_locus_tag="pgaptmp_003174"
     CDS             complement(6017..7519)
                     /gene="proP"
                     /locus_tag="ACHOSC_RS15870"
                     /old_locus_tag="pgaptmp_003174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709830.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine/L-proline transporter ProP"
                     /protein_id="HGU4549071.1"
                     /translation="MLKRKKVKPITLRDVTIIDDGKLRKAITAASLGNAMEWFDFGVY
                     GFVAYALGKVFFPGADPSVQMVAALATFSVPFLIRPLGGLFFGMLGDKYGRQKILAIT
                     IVIMSISTFCIGLIPSYDTIGIWAPILLLICKMAQGFSVGGEYTGASIFVAEYSPDRK
                     RGFMGSWLDFGSIAGFVLGAGVVVLISTIVGEANFLDWGWRIPFFIALPLGIIGLYLR
                     HALEETPAFQQHVDKLEQGDREGLQDGPKVSFKEIATKHWRSLLTCIGLVIATNVTYY
                     MLLTYMPSYLSHNLHYSEDHGVLIIIAIMIGMLFVQPVMGLLSDRFGRRPFVLLGSVA
                     LFVLAIPAFILINSNVIGLIFAGLLMLAVILNCFTGVMASTLPAMFPTHIRYSALAAA
                     FNISVLVAGLTPTLAAWLVESSQNLMMPAYYLMVVAVIGLITGVTMKETANRPLKGAT
                     PAASDIQEAKEILVEHYDNIEQKIDNIDHEIADLQAKRTRLVQQHPRIDE"
     gene            complement(7783..8661)
                     /gene="rdcB"
                     /locus_tag="ACHOSC_RS15875"
                     /old_locus_tag="pgaptmp_003175"
     CDS             complement(7783..8661)
                     /gene="rdcB"
                     /locus_tag="ACHOSC_RS15875"
                     /old_locus_tag="pgaptmp_003175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418534.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjcZ-like family protein"
                     /protein_id="HGU4549072.1"
                     /translation="MTKTLLDGPGRVLESVYPRFLVDLAQGDDARLPQAHQQQFRERL
                     MQELLARVQLQTWTNGGMLNAPLSLRLTLVEKLASMLDPGHLALTQIAQHLALLQKMD
                     HRQHSAFPELPQQIAALYEWFSARCRWKEKALTQRGLLVQAGDQSEQIFTRWRAGAYN
                     AWSLPGRCFIVLEELRWGAFGDACRLGSPQAVALLLGDLRVKATQHLAESINAAPTTR
                     HYYHQWFASSTVPTGGDHADFLSWLGKWTTADKQPVCWSVTQRWQTVALGMPRLCSAQ
                     RLAGAMLEEIFSVNLA"
     gene            complement(8658..>10865)
                     /gene="crfC"
                     /locus_tag="ACHOSC_RS15880"
                     /old_locus_tag="pgaptmp_003176"
     CDS             complement(8658..>10865)
                     /gene="crfC"
                     /locus_tag="ACHOSC_RS15880"
                     /old_locus_tag="pgaptmp_003176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="clamp-binding protein CrfC"
                     /protein_id="HGU4549073.1"
                     /translation="ELSQEAERLLQLSRQQLQLLEKMPLSVPGDDAPQLALPWSQPNI
                     AERHAMLNNELRKISRLEMVLAIVGTMKAGKSTTINAIVGTEVLPNRNRPMTALPTLI
                     RHTPGQKEPVLHFSHVAPIDRLIQKLQQRLRDCDIKHLTDVLEIDKDMRALMQRIENG
                     VAFEKYYLGAQPIFHCLKSLNDLVRLAKALDVDFPFSAYAAIEHIPVIEVEFVHLAGL
                     ESYPGQLTLLDTPGPNEAGQPHLQKMLNQQLARASAVLAVLDYTQLKSISDEEVREAI
                     LAVGQSVPLYVLVNKFDQQDRNSDDADQVRALISGTLMKGCITPQQIFPVSSMWGYLA
                     NRARHELANNGKLPPPEQQRWVEDFAHAALGRRWRHADLADLEHIRHAADQLWEDSLF
                     AQPIQALLHAAYANASLYALRSAAHKLLNYAQQAREYLDFRAHGLNVACEQLRQNIHQ
                     VEESLQLLQLNQAQVSGEIKHEIELALASANHFLRQQQDAVNAQLAALFQDDSEPLSE
                     IRTRCETLLQTAQNTISRDFTLRFAELESTLCRVLTDVIRPIEQQVKMELSESGFRPG
                     FHFPVFHGAVPHFNTRQLFSEVISRQEATDEQSTRLGVVRETFSRWLNQPDWGRGNEK
                     SPTETVDYSVLQRALSAEVDLYCQQMAKVLAEQVDESVTAGMNTFFAEFASCLTELQT
                     RLRESLALRQQNESVVRLMQQQLQRTVMTHGWIYTDAQLLRDDIQTLFTAERY"
BASE COUNT         2712 a         2841 c         2686 g         2626 t
ORIGIN      
        1 tttatattca tcatacaatt atataaccat ttcccggtat cgctttgctt tagcgagaac
       61 tggtctttta tttgcactca ggagtacatg tatgaggatt tgcagcgacc aaccttgtat
      121 tgttttattg actgaaaaag atgtctggat aagggtgaat gggaaagaac ctattagcct
      181 taaagctaac catatggcgt tattaaattg tgaaaataat attatcgacg tctcctctct
      241 taacaacact ttggttgctc atattagtca cgacatcatc aaagattacc tccggtttct
      301 gaataaagat ctctcgcaaa taccagtatg gcaacgtagc gctacgccca tactcaccct
      361 gccatgcctg acgccagacg tctttcgcgt tgccgcgcaa cacagcatga tgcccgcaga
      421 aactgagtca gaaaaggaac gaacacgcgc attattattc acggtgctat cccgttttct
      481 cgacagtaaa aaattccttt cattaatgat gtatatgtta cgtaattgtg taagtgacag
      541 cgtttatcaa attattgaaa gcgatattca caaagactgg aatcttagta tggtagccag
      601 ttgtttatgt cttagcccaa gtctgttaaa gaaaaagttg aaaagcgaaa acaccagtta
      661 tagccaaata atcaccacct gccgcatgcg ttatgccgta aatgaattaa tgatggacgg
      721 taaaaatatc tctcaggtat cacagtcctg cggctacaac agtacgtcgt actttatttc
      781 tgtctttaaa gacttctacg gtatgacgcc gctgcattat gttagtcagc acagagaacg
      841 cactgtcgcc tgatttttaa ccttaacgaa gagctatatt aataacggca tcagcgataa
      901 cccggtcgat aataattcaa ctatcgaatg caggcgtatg atatgacgta atttattgtc
      961 acgaagctcg ccttcgcagg agtttaatta tgtcttcgga tgctgatgct cacaaagtgg
     1021 gcttaatccc cgtcaccctg atggtgtcgg ggaatattat ggggtcaggt gtttttctgt
     1081 tacctgcaaa cctggcctct actggcggga ttgccattta tggatggttg gtgacgatta
     1141 tcggtgcgct ggggctctcg atggtatacg ccaaaatgtc gttcctcgat ccaagtcctg
     1201 gtggttctta cgcgtacgcc cgccgctgct ttggcccgtt tctcggttat caaaccaacg
     1261 tcctctactg gctggcctgc tggatcggca atatcgccat ggtggtcatt ggcgtaggat
     1321 atttaagtta cttcttcccg attctgaaag atccattggt attaaccatc acctgcgtcg
     1381 tggtgctgtg gatcttcgtc ctgctgaaca ttgtcggtcc gaaaatgatc acccgtgtgc
     1441 aggccgttgc caccgtgctg gcgctgatcc ccatcgtcgg gattgccgta tttggctggt
     1501 tctggttccg tggtgaaacc tatatggcgg catggaacgt cagcggcctg ggcaccttcg
     1561 gtgcaattca aagtaccctt aacgttacgc tgtggtcgtt catcggtgtg gaaagtgcct
     1621 ccgttgccgc aggtgtggtg aaaaacccga aacgcaatgt ccctatcgcc accattggtg
     1681 gggtattgat tgccgccgtt tgctatgtac tttctaccac cgcgattatg gggatgatcc
     1741 ctaatgccgc actgcgcgtt tctgcttcgc cattcggtga tgccgcacgg atggcgttgg
     1801 gtgacaccgc cggggccatt gtttccttct gcgcagctgc gggttgctta ggttcactgg
     1861 gcggctggac gttgctggcg ggtcaaacgg cgaaagccgc tgccgatgac ggactgttcc
     1921 caccgatttt tgcccgtgta aataaagcgg gcacgccagt ggcggggctg attatcgtcg
     1981 gtattttgat gaccatcttc cagctcagca gcatttcacc aaacgcgacc aaagagttcg
     2041 gtctggtttc ttccgtctcg gtcatcttta cactggtgcc atatctttac acctgtgcgg
     2101 cgttactgct gctcggtcac ggtcactttg gtaaagcacg cccggcatat ctggctgtta
     2161 ctaccattgc cttcctctac tgcatttggg ccgtggtggg gtccggggcg aaagaggtta
     2221 tgtggtcatt tgtcaccctg atggtaatca ccgccatgta tgcgctgaat tacaaccggc
     2281 tgcataaaaa cccgtatccc ttagatgtac caataagcaa agattaattc cccttaatcc
     2341 agcaaacata aaagccaacc ttaagaactt aaggttggct taattttgct ttgcgagcat
     2401 atgcgcactt tgttcgatgg aaacaccgtg atgttgaagc gcctactaaa aagaccctct
     2461 ttgaatttac tcgcctggct attgttggcc gctttttata tctctatctg cctgaatatt
     2521 gcctttttta aacaggtgtt gcaggcgctg ccgctggatt cgctgcataa cgtactggtt
     2581 ttcttgtcga tgccggtcgt cgctttcagc gtgattaata ttgtcctgac actaagctct
     2641 ttcttatggc ttaatcgacc actggcctgc ctgtttattc tggttggcgc ggctgcacaa
     2701 tatttcataa tgacttacgg catcgtcatc gaccgctcga tgattgccaa tattattgat
     2761 accactccgg cagaaagtta tgcgctgatg acaccgcaaa tgttattaac gctgggattc
     2821 agcggcgtgc ttgctgcgct gattgcctgc tggataaaaa tcaaacctgc cacctcgcgt
     2881 ctgcgcagtg ttcttttccg tggagccaat attctggttt ctgtactact gattttgctg
     2941 gtcgccgcac tgttttataa agactacgcc tcgttgttcc gcaataacaa agagctggtg
     3001 aaatccttaa gcccctctaa cagcattgtt gccagctggt catggtactc ccatcagcga
     3061 ctggcaaatc taccgctggt gcgaattggt gaagacgccc accgcaaccc gttaatgcag
     3121 aacgaaaaac gtaaaaattt gaccatcctg attgtcggcg aaacctcgcg ggcggagaac
     3181 ttctccctca acggctaccc gcgtgaaact aacccgcggc tggcgaaaga taacgtggtc
     3241 tatttcccta ataccgcatc ttgcggcacg gcaacggcag tttcagtacc gtgcatgttc
     3301 tcggatatgc cgcgtgagca ttacaaagaa gagctggcac agcaccagga aggcgtgctg
     3361 gatatcattc agcgagcggg catcaacgtg ctgtggaatg acaacgatgg cggctgtaaa
     3421 ggtgcctgcg accgcgtgcc tcaccagaac gtcaccgcgc tgaatctgcc tggtcagtgc
     3481 atcaacggcg aatgctatga cgaagtgctg ttccacgggc tggaagagta catcaataac
     3541 ctgcagggtg atggcgtgat tgtcttacac accatcggca gccacggtcc gacctattac
     3601 aaccgctatc cgccgcagtt caggaaattt accccaacct gcgacaccaa tgagatccag
     3661 acctgtagca aagagcaact ggtgaacact tacgacaaca cgctggttta cgtcgactat
     3721 attgttgata aagcgattaa tctgctgaaa gaacatcagg ataaatttac caccagcctg
     3781 gtttatcttt ctgaccacgg tgaatcgtta ggtgaaaatg gcatctatct gcacggtctg
     3841 ccttatgcca tcgccccgga tagccaaaaa caggtgccga tgctgctgtg gctgtcggag
     3901 gattatcaaa aacggtatca ggttgaccag aactgcctgc aaaaacaggc gcaaacgcaa
     3961 cactattcac aagacaattt attctccacg ctattgggat taactggcgt tgagacgaag
     4021 tattaccagg ctgcggatga tattctgcaa acttgcagga gagtgagtga atgaaaattc
     4081 tgattgttga agacgatacg ctgttattgc agggattgat tctggcggcg caaactgaag
     4141 gctacgcgtg cgatggcgtg acaaccgcgc ggatggcgga acaaagcctt gaggccggtc
     4201 attacagcct ggtggtactg gatttagggt tacccgacga agatggactg cattttctcg
     4261 cccgtatccg gcagaaaaaa tacaccctgc cagtactgat cctcaccgct cgcgatacgc
     4321 tgaccgacaa aatcgctggg ctggatgtcg gtgccgatga ctatctggtg aagccttttg
     4381 cgctggaaga gttacacgcc cgtatccgcg ccctgctacg acgccataat aatcagggcg
     4441 aaagtgagct gattgttggc aatctgacgc tgaatatggg tcgccgtcag gtatggatgg
     4501 gcggtgaaga gttgattctg acgcccaaag aatatgctct gctgtcacgg ttaatgctca
     4561 aagcaggcag tccggtgcat cgggaaattc tctacaacga catctataac tgggacaatg
     4621 aaccctcgac caacaccctg gaagtgcata tccacaatct gcgcgacaaa gtgggcaaag
     4681 cccgtatccg caccgtgcgc ggctttggct atatgctggt cgcgaatgag gaaaactaat
     4741 tgaatctgat gcattttctg cgccgaccaa tatcgctgcg ccaacggctg atattgacca
     4801 tcggggccat tttgttggtg tttgagctga tcagcgtctt ctggctatgg catgaaagta
     4861 ccgagcagat tcagctgttt gagcaggcgc tacgcgacaa tcgcaacaac gatcgccaca
     4921 tcatgcgtga gatccgcgag gcggtcgcca gcctgattgt ccccggcgtc tttatggtca
     4981 gcctgacgct atttatctgc tatcaggcgg tgcgccgcat cacccgcccg ctggcggagc
     5041 tgcaaaaaga gctggaagcg cgcactgccg acaacttaac gcccattgcc attcacagcg
     5101 ccaccctcga aatcgaagcg gtggtttcgg cgttaaacga tctggtcagt cgcctgacca
     5161 gcacgctgga taacgaaagg ttgtttaccg ctgacgtcgc gcacgaactg cgaacgccac
     5221 tggcgggggt gcgtttgcat ctggaactgc tggcgaaaac gcatcacatt gatgtagcac
     5281 cgttagtggc acgacttgat cagatgatgg agagcgtctc ccagctgctg caactggcgc
     5341 gtgccggaca gtcattttct tccggtaatt atcaacatgt aaaactgctg gaagatgtca
     5401 ttctcccctc gtatgacgaa ctcagtacca tgctcgacca gcgacagcaa accctgctat
     5461 tgccagagag cgccgccgac atcaccgttc agggtgatgc aaccctgctg cggatgttat
     5521 tgcgaaacct ggtagaaaac gcccatcgtt acagcccgca aggcagcaac attatgatta
     5581 agctgcaaga agatggcgga gcggtcatgg cagttgaaga tgaaggacca ggtattgatg
     5641 agagtaaatg cggggagttg agtaaagcgt ttgtacgtat ggacagccgt tatggcggga
     5701 ttggtctggg gttaagtatt gtcagccgca ttacacagtt gcatcacggg cagtttttcc
     5761 tgcaaaaccg gcaagagact tccggcacgc gggcctgggt acggctgaag aaagatcaga
     5821 acgtggcaaa ccagatataa agaaagctgc tgaccaccag cacgctgaac acggtagtta
     5881 aactttcata aacacggttt ttcattgcac tctcctccgt taacctggag gagagtatgc
     5941 gcgtcagaga ttaagtgaac cttaagagtt caattggcct gataagacag cgtcacatca
     6001 ggccatccgt ttcagcttat tcatcaattc gcggatgttg ctgcaccagg cgggtacgtt
     6061 tcgcctgcaa atcggcaatc tcgtggtcaa tattatcgat tttctgctcg atattatcgt
     6121 aatgctcgac gagaatttcc ttcgcttcct gtatatctga cgccgccggt gtcgcacctt
     6181 tcaacggacg atttgccgtc tctttcatgg ttacgccggt gattaaacca atcaccgcca
     6241 ctaccatcag gtaataggca ggcatcatca gattctgcga gctttcgacc agccaggccg
     6301 ccagtgttgg cgtcagaccg gcaaccagca ccgaaatatt aaatgccgcc gccagcgcgc
     6361 tgtaacggat atgcgtcggg aacatcgctg gcaaggtaga agccataacg cccgtaaagc
     6421 agttaaggat caccgccagc atcagtaacc cggcaaaaat caggccgatg acgttactgt
     6481 taatcagaat aaacgccggg atcgccaaca caaacagggc aacactacca agtagcacaa
     6541 acggacgacg gccaaaacgg tcactcagca agcccatcac cggctggaca aacagcatac
     6601 cgatcataat ggcgataata atcagcaccc cgtggtcttc ggagtaatgc aggttatgcg
     6661 acaaataact cggcatatag gtcagcaaca tgtagtaagt cacgttggtg gcaattacca
     6721 gaccaataca tgtcaacagg ctgcgccagt gtttggtggc aatctcttta aacgagactt
     6781 tcgggccatc ctgcaaacct tcgcggtcgc cctgttccag tttatcgaca tgctgctgga
     6841 acgccggagt ttcttccagc gcatggcgca ggtaaagccc gataatccct aacggcagag
     6901 caataaagaa cggaatacgc cagccccagt cgaggaagtt cgcttcgccg acaatggtcg
     6961 aaattaacac cactacgcca gcacccagca caaacccggc aatagaaccg aagtccagcc
     7021 agctgcccat aaagccacgt ttacggtcag gtgagtattc cgcaacaaat atcgacgccc
     7081 cggtatattc accgccgacc gagaaacctt gtgccatctt acagatcaac agcagaatcg
     7141 gtgcccaaat accaatcgtg tcgtaggacg gtattaagcc aatacagaac gtactgatcg
     7201 acataatcac aatagtgata gcgaggatct tctggcgacc atatttatcg cccaacatac
     7261 caaagaagag cccgccaagc ggtcgaatca gaaagggaac ggagaaagtg gcaagtgcag
     7321 caaccatctg cacgctgggg tcagcccccg ggaaaaaaac tttacctaat gcgtaagcaa
     7381 caaaaccata aacaccaaaa tcaaaccatt ccattgcatt acccagtgat gctgcggtaa
     7441 tggctttacg cagtttaccg tcatcaataa tggtgacatc acgaagggta attggtttta
     7501 cttttttcct tttcagcata gctttcctcg cagagttggc ccatttccgc aaacgccaga
     7561 aaaatcagcg cgacggccgc aaaaggggag cgggaattgc aggatgcaat ctctgtaaca
     7621 agcgtagcgg gtttgcttac accctccggt taaggaatgt acaatcaaac cagttgacgc
     7681 ctgaattggg cagttaatga cctttttacc ctaccagtgt ttaaatttgt gatcaacttc
     7741 acacatggat ttcgtttagg actcattgat gtaactgatt atttacgcca aatttacaga
     7801 gaagatttcc tcgagcattg cccccgccag acgctgcgct gaacagagtc gtggcatccc
     7861 cagcgcgaca gtttgccagc gttgggtcac tgaccagcaa acgggttgtt tatctgccgt
     7921 ggtccacttt cccagccaac ttaaaaaatc agcatgatcc ccgcccgtcg gaacggtcga
     7981 agaggcaaac cactgatggt aatagtgacg cgtggtcggg gccgcattga tactctctgc
     8041 cagatgttgt gtcgctttca cgcgcaaatc acccagcaac aacgccaccg cttgcgggct
     8101 tcccagacga caggcatcgc caaatgcccc ccaacgcaac tcttcgagaa cgataaaaca
     8161 gcgcccaggc aacgaccagg cgttatacgc cccagcacgc cagcgggtaa aaatttgctc
     8221 gctctgatca cctgcctgca ccagtaggcc tcgttgcgtt aacgcctttt ccttccagcg
     8281 gcaacgggct gaaaaccact catacaaggc ggcaatttgc tgggggagct ccgggaaagc
     8341 agagtgctgg cggtgatcca ttttttgcag cagcgccaga tgctgcgcga tctgcgtcag
     8401 tgccagatga ccgggatcca gcatcgacgc cagtttttcc accaatgtca gacgcaggct
     8461 aagcggcgca tttaacatgc cgccgttcgt ccatgtctga agctgcacgc gcgcaagaag
     8521 ttcctgcatc agtcgttcac gaaactgctg ctgatgggct tgtggaaggc gggcatcatc
     8581 gccctgcgcc agatccacta aaaagcgggg ataaaccgac tccagcacgc gaccggggcc
     8641 gtcaagtaac gtcttggtca atatcgttct gccgtgaaaa gtgtttgaat atcatcgcgt
     8701 aacagctggg cgtcggtgta aatccagccg tgagtcatca cagtccgctg caattgctgc
     8761 tgcatcagcc tgaccaccga ttcattttgt tgacgcagag ccagactttc gcgtaaacgc
     8821 gtctgtaatt ccgtcaaaca tgaagcgaac tcagcgaaaa aagtattcat gcctgccgta
     8881 acagattcat cgacctgctc tgccagaact ttagccattt gttggcaata aagatcgact
     8941 tctgcgctta aagctcgttg caacacactg taatcaaccg tctctgtcgg ggatttctca
     9001 tttccccgtc cccagtcggg ctgattcaac cagcgcgaaa aagtctcacg cacaacgcct
     9061 aaacgcgtgc tctgctcgtc cgttgcttcc tggcgcgaaa tgacttcact gaacagctgg
     9121 cgagtgttga agtggggaac tgcgccgtga aaaacaggaa aatgaaaccc aggacgaaac
     9181 cctgactcgc tcaattccat tttgacttgt tgctcaatgg ggcgaataac atcggttaac
     9241 acccggcaaa gggtggattc cagctcggca aaacgcagcg taaagtcgcg actgatggtg
     9301 ttctgcgccg tctgtaacag tgtctcacag cgggtacgaa tctcgcttaa cggctccgaa
     9361 tcatcctgaa acaacgcggc taactgtgca ttcaccgcat cttgctgttg acgcagaaag
     9421 tggttggcgg aggccaaggc cagctcgatt tcatgtttaa tctcgccgct cacctgcgcc
     9481 tgattgagtt gcaatagctg caaactttct tcgacctgat ggatattttg ccgcaattgt
     9541 tcacaagcga cgtttaaccc gtgcgcacga aaatccaggt attcccgcgc ctgctgcgcg
     9601 taattcaaca gtttatgcgc ggcagatcgc agagcataca acgaggcgtt agcgtaagcg
     9661 gcatgaagca acgcctgaat tggctgggcg aacagcgaat cttcccacaa ctgatcggca
     9721 gcatgacgaa tatgttcgag gtccgccaga tcggcatgac gccagcgcct gccgagcgcg
     9781 gcatgggcaa aatcttccac ccagcgttgt tgctctggcg gtggtaactt accgttgttg
     9841 gctaactcat ggcgcgcccg attcgccagg tagccccaca tcgacgacac cggaaatatc
     9901 tgctgtggcg taatacagcc tttcatcagc gtcccggaaa tcagtgcccg cacctggtcg
     9961 gcgtcgtcac tgttacgatc ctgttgatcg aacttattga ccagcacata cagcggcacc
    10021 gattgcccca ccgccaaaat cgcctcacgg acctcttcat cggagatcga tttcagttgc
    10081 gtataatcca gcaccgccag taccgccgag gcgcgtgcca gctgctggtt aagcattttt
    10141 tgcagatgcg gttgcccggc ttcatttggc ccgggggtat ccagtaacgt caactgaccg
    10201 ggataacttt ccagccccgc cagatggaca aactccactt caatcacggg aatatgctca
    10261 atggcggcgt aagcagaaaa aggaaaatcg acgtccagcg ccttcgccag tcgcactaaa
    10321 tcattcaaac ttttcagaca atgaaaaata ggctgggcac ccagataata tttttcgaaa
    10381 gcgacgccat tttcgatccg ctgcataagc gcacgcatat ctttatctat ttccagcaca
    10441 tcggtcagat gcttaatatc gcaatcacgc aggcgctgtt gtaatttttg aattaaacgg
    10501 tcgattggcg cgacatgtga aaaatgcagt accggttctt tttgcccggg cgtatggcga
    10561 ataagcgtcg gcagcgcagt cattgggcga ttacgattag gcaaaacctc cgtaccaaca
    10621 atggcattaa tggtggttga tttccctgct ttcatggtac cgacaatcgc cagcaccatt
    10681 tccagtcggg aaattttacg caactcatta ttcagcatcg cgtgacgttc ggcgatatta
    10741 ggctgactcc agggtaaagc cagttgtggc gcatcgtctc cgggtacaga gagaggcatt
    10801 ttttccagta actgcaactg ttggcgagaa agctgtaaca ggcgttcagc ctcctgactt
    10861 aactc
//
LOCUS       DAZKHF020000153        10728 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2201S31241
            SAMN43267800-rid25106413.denovo.154, whole genome shotgun sequence.
ACCESSION   DAZKHF020000153 DAZKHF020000000
VERSION     DAZKHF020000153.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267800
            Sequence Read Archive: SRR30307263
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 10728)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10728)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 10728)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350581.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350581.2
            Genome Coverage       :: 151x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:21:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,652
            CDSs (total)                      :: 5,557
            Genes (coding)                    :: 5,320
            CDSs (with protein)               :: 5,320
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 237
            CDSs (without protein)            :: 237
            Pseudo Genes (ambiguous residues) :: 0 of 237
            Pseudo Genes (frameshifted)       :: 102 of 237
            Pseudo Genes (incomplete)         :: 130 of 237
            Pseudo Genes (internal stop)      :: 52 of 237
            Pseudo Genes (multiple problems)  :: 41 of 237
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10728
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267800-rid25106413.denovo.154"
                     /serotype="O 26:H11"
                     /isolate="2201S31241"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-01"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            complement(47..400)
                     /locus_tag="ACHOSC_RS15885"
                     /old_locus_tag="pgaptmp_003177"
     CDS             complement(47..400)
                     /locus_tag="ACHOSC_RS15885"
                     /old_locus_tag="pgaptmp_003177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418568.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4156 domain-containing protein"
                     /protein_id="HGU4549074.1"
                     /translation="MHVKYLAGIVGAALLMAGCSSSNELSAAGQSVRIVDEQPGAECQ
                     LIGTATGKQSNWLSGQHGEEGGSMRGAANDLRNQAAAMGGNVIYGISSPSQGMLSSFV
                     PTDSQIIGQVYKCPN"
     gene            complement(538..2184)
                     /gene="groL"
                     /locus_tag="ACHOSC_RS15890"
                     /old_locus_tag="pgaptmp_003178"
     CDS             complement(538..2184)
                     /gene="groL"
                     /locus_tag="ACHOSC_RS15890"
                     /old_locus_tag="pgaptmp_003178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005120975.1"
                     /note="60 kDa chaperone family; promotes refolding of
                     misfolded polypeptides especially under stressful
                     conditions; forms two stacked rings of heptamers to form a
                     barrel-shaped 14mer; ends can be capped by GroES;
                     misfolded proteins enter the barrel where they are
                     refolded when GroES binds; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0140662 - ATP-dependent protein folding
                     chaperone [Evidence IEA];
                     GO_process: GO:0042026 - protein refolding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chaperonin GroEL"
                     /protein_id="HGU4549075.1"
                     /translation="MAAKDVKFGNDARVKMLRGVNVLADAVKVTLGPKGRNVVLDKSF
                     GAPTITKDGVSVAREIELEDKFENMGAQMVKEVASKANDAAGDGTTTATVLAQAIITE
                     GLKAVAAGMNPMDLKRGIDKAVTAAVEELKALSVPCSDSKAIAQVGTISANSDETVGK
                     LIAEAMDKVGKEGVITVEDGTGLQDELDVVEGMQFDRGYLSPYFINKPETGAVELESP
                     FILLADKKISNIREMLPVLEAVAKAGKPLLIIAEDVEGEALATLVVNTMRGIVKVAAV
                     KAPGFGDRRKAMLQDIATLTGGTVISEEIGMELEKATLEDLGQAKRVVINKDTTTIID
                     GVGEEAAIQGRVAQIRQQIEEATSDYDREKLQERVAKLAGGVAVIKVGAATEVEMKEK
                     KARVEDALHATRAAVEEGVVAGGGVALIRVASKLADLRGQNEDQNVGIKVALRAMEAP
                     LRQIVLNCGEEPSVVANTVKGGDGNYGYNAATEEYGNMIDMGILDPTKVTRSALQYAA
                     SVAGLMITTECMVTDLPKNDAADLGAAGGMGGMGGMGGMM"
     gene            complement(2228..2521)
                     /locus_tag="ACHOSC_RS15895"
                     /old_locus_tag="pgaptmp_003179"
     CDS             complement(2228..2521)
                     /locus_tag="ACHOSC_RS15895"
                     /old_locus_tag="pgaptmp_003179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000027827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0140662 - ATP-dependent protein folding
                     chaperone [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone GroES"
                     /protein_id="HGU4549076.1"
                     /translation="MNIRPLHDRVIVKRKEVETKSAGGIVLTGSAAAKSTRGEVLAVG
                     NGRILENGEVKPLDVKVGDIVIFNDGYGVKSEKIDNEEVLIMSESDILAIVEA"
     gene            2797..4053
                     /gene="yjeH"
                     /locus_tag="ACHOSC_RS15900"
                     /old_locus_tag="pgaptmp_003180"
     CDS             2797..4053
                     /gene="yjeH"
                     /locus_tag="ACHOSC_RS15900"
                     /old_locus_tag="pgaptmp_003180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-methionine/branched-chain amino acid
                     transporter"
                     /protein_id="HGU4549077.1"
                     /translation="MSGLKQELGLAQGIGLLSTSLLGTGVFAVPALAALVAGNNSLWA
                     WPVLIILVFPIAIVFAILGRHYPSAGGVAHFVGMAFGSRLERVTGWLFLSVIPVGLPA
                     ALQIAAGFGQAMFGWHSWQLLLAELGTLALVWYIGTRGASSSANLQTVIAGLIVALIV
                     AIWWAGDIKPANIPFPAPGNIELTGLFAALSVMFWCFVGLEAFAHLASEFKNPERDFP
                     RALMIGLLLAGLVYWGCTVVVLHFDAYGEKMAAAASLPKIVVQLFGVGALWIACVIGY
                     LACFASLNIYIQSFARLVWSQAQHNPDHYLARLSSRHIPNNALNAVLGCCVVSTLVIH
                     ALEINLDALIIYANGIFIMIYLLCMLAGCKLLQGRYRLLAVVGGLLCVLLLAMVGWKS
                     LYALIMLAGLWLLLPKRKTPENGITT"
     gene            complement(4069..4545)
                     /locus_tag="ACHOSC_RS15905"
                     /old_locus_tag="pgaptmp_003181"
     CDS             complement(4069..4545)
                     /locus_tag="ACHOSC_RS15905"
                     /old_locus_tag="pgaptmp_003181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418564.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FxsA family protein"
                     /protein_id="HGU4549078.1"
                     /translation="MRWLPFIAIFLYVYIEISIFIQVAHVLGVLLTLVLVIFTSVIGM
                     SLVRNQGFKNFVLMQQKMAAGENPAAEMIKSVSLIIAGLLLLLPGFFTDFLGLLLLLP
                     PVQKHLTVKLMPHLRFSRMPGGGFSAGTGGGNTFDGEYQRKDDERDRLDHKDDRQD"
     gene            4882..6318
                     /gene="aspA"
                     /locus_tag="ACHOSC_RS15910"
                     /old_locus_tag="pgaptmp_003182"
     CDS             4882..6318
                     /gene="aspA"
                     /locus_tag="ACHOSC_RS15910"
                     /old_locus_tag="pgaptmp_003182"
                     /EC_number="4.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313147.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008797 - aspartate ammonia-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate ammonia-lyase"
                     /protein_id="HGU4549079.1"
                     /translation="MSNNIRIEEDLLGTREVPADAYYGVHTLRAIENFYISNNKISDI
                     PEFVRGMVMVKKAAAMANKELQTIPKSVANAIIAACDEVLNNGKCMDQFPVDVYQGGA
                     GTSVNMNTNEVLANIGLELMGHQKGEYQYLNPNDHVNKCQSTNDAYPTGFRIAVYSSL
                     IKLVDAINQLREGFERKAVEFQDILKMGRTQLQDAVPMTLGQEFRAFSILLKEEVKNI
                     QRTAELLLEVNLGATAIGTGLNTPKEYSPLAVKKLAEVTGFPCVPAEDLIEATSDCGA
                     YVMVHGALKRLAVKMSKICNDLRLLSSGPRAGLNEINLPELQAGSSIMPAKVNPVVPE
                     VVNQVCFKVIGNDTTVTMAAEAGQLQLNVMEPVIGQAMFESVHILTNACYNLLEKCIN
                     GITANKEVCEGYVYNSIGIVTYLNPFIGHHNGDIVGKICAETGKSVREVVLERGLLTE
                     AELDDIFSVQNLMHPAYKAKRYTDESEQ"
     gene            6436..7737
                     /gene="dcuA"
                     /locus_tag="ACHOSC_RS15915"
                     /old_locus_tag="pgaptmp_003183"
     CDS             6436..7737
                     /gene="dcuA"
                     /locus_tag="ACHOSC_RS15915"
                     /old_locus_tag="pgaptmp_003183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic C4-dicarboxylate transporter DcuA"
                     /protein_id="HGU4549080.1"
                     /translation="MLVVELIIVLLAIFLGARLGGIGIGFAGGLGVLVLAAIGVKPGN
                     IPFDVISIIMAVIAAISAMQVAGGLDYLVHQTEKLLRRNPKYITILAPIVTYFLTIFA
                     GTGNISLATLPVIAEVAKEQGVKPCRPLSTAVVSAQIAITASPISAAVVYMSSVMEGH
                     GISYLHLLSVVIPSTLLAVLVMSFLVTMLFNSKLSDDPIYRKRLEEGLVELRGEKQIE
                     IKSGAKTSVWLFLLGVVGVVIYAIINSPSMGLVEKPLMNTTNAILIIMLSVATLTTVI
                     CKVDTDNILNSSTFKAGMSACICILGVAWLGDTFVSNNIDWIKDTAGEVIQGHPWLLA
                     VIFFFASALLYSQAATAKALMPMALALNVSPLTAVASFAAVSGLFILPTYPTLVAAVQ
                     MDDTGTTRIGKFVFNHPFFIPGTLGVALAVCFGFVLGSFML"
     gene            7853..8191
                     /gene="cutA"
                     /locus_tag="ACHOSC_RS15920"
                     /old_locus_tag="pgaptmp_003184"
     CDS             7853..8191
                     /gene="cutA"
                     /locus_tag="ACHOSC_RS15920"
                     /old_locus_tag="pgaptmp_003184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="divalent cation tolerance protein CutA"
                     /protein_id="HGU4549081.1"
                     /translation="MLDEESSNTASVVVLCTAPDEATAQDLAAKVLAEKLAACATLIP
                     GATSLYYWEGKLEQEYEVQMILKTTVSHQQALLECLKSHHPYQTPELLVLPVTHGDTD
                     YLSWLNASLR"
     gene            8167..9864
                     /gene="dsbD"
                     /locus_tag="ACHOSC_RS15925"
                     /old_locus_tag="pgaptmp_003185"
     CDS             8167..9864
                     /gene="dsbD"
                     /locus_tag="ACHOSC_RS15925"
                     /old_locus_tag="pgaptmp_003185"
                     /EC_number="1.8.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-disulfide reductase DsbD"
                     /protein_id="HGU4549082.1"
                     /translation="MAQRIFTLILLLCSTSVFAGLFDAPGRSHFVPADQAFAFDFQQN
                     QHDLNLTWQIKDGYYLYRKQIRITPEHAKIADVQLPQGVWHEDEFYGKSEIYRDRLTL
                     PVTINQASAGATLTVTYQGCADAGFCYPPETKTVPLSEVVANNAASQPVSVPQQEQHT
                     AQLPFSALWALLIGIGIAFTPCVLPMYPLISGIVLGGKQRLSTARALLLTFIYVQGMA
                     LTYTALGLVVAAAGLQFQAALQHPYVLIGLAIVFTLLAMSMFGLFTLQLPSSLQTRLT
                     LMSNRQQGGSPGGVFVMGAIAGLICSPCTTAPLSAILLYIAQSGNMWLGGGTLYLYAL
                     GMGLPLMLITVFGNRLLPKSGPWMEQVKTAFGFVILALPVFLLERVIGDVWGLRLWSA
                     LGVAFFGWAFITSLQAKRGWMRVVQIILLAAALVSVRPLQDWAFGATHTAQTQTHLNF
                     TQIKTVDELNQALVEVKGKPVMLDLYADWCVACKEFEKYTFSDPQVQKALADTVLLQA
                     NVTANDAQDVALLKHLNVLGLPTILFFDGQGQEHPQARVTGFMDAETFSAHLRDRQP"
     gene            9901..10476
                     /locus_tag="ACHOSC_RS15930"
                     /old_locus_tag="pgaptmp_003186"
     CDS             9901..10476
                     /locus_tag="ACHOSC_RS15930"
                     /old_locus_tag="pgaptmp_003186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HGU4549083.1"
                     /translation="MQREDVLGEALKLLELQGIANTTLEMVAERVDYPLDELRRFWPD
                     KEAILYDALRYLSQQIDVWRRQLMLDETQTAEQKLLARYQALSECVKNNRYPGCLFIA
                     ACTFYPDPGHPIHQLADQQKSAAYDFTHELLTTLEVDDPAMVAKQMELVLEGCLSRML
                     VNRSQADVDTAHRLAEDILRFARCRQGGALT"
     gene            10583..10658
                     /locus_tag="ACHOSC_RS15935"
                     /old_locus_tag="pgaptmp_003187"
     tRNA            10583..10658
                     /locus_tag="ACHOSC_RS15935"
                     /old_locus_tag="pgaptmp_003187"
                     /product="tRNA-Phe"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:10616..10618,aa:Phe,seq:gaa)
BASE COUNT         2496 a         2819 c         2680 g         2733 t
ORIGIN      
        1 agcgcatcag gcaatgatac gtttgtcacc cagcctctgg atcacatcag ttcgggcact
       61 tataaacctg accgataatc tggctatccg tcgggacaaa actggacaac attccctgcg
      121 acgggctgct gatgccataa atcacgttac cgcccattgc agccgcctgg ttgcgcagat
      181 cgtttgctgc gccgcgcata gaaccgccct cttctccgtg ttgcccggaa agccagttgc
      241 tttgcttacc tgtcgcagta ccaatcagct ggcactctgc gcctggctgc tcgtccacaa
      301 tgcgtacact ctgaccggca gcactcaatt cgttgctgga gctacaaccc gccatcagta
      361 gcgcggcacc gacaatccct gctaagtatt ttacgtgcat gttattcccc ataatcaatg
      421 agctggacgc cactcgcgtc gtccgtgtct gaatcttata ctagaaagat gaccaaaaga
      481 aaaaccccca gacatttctg cccgggggtt tgtttatttc tgcgaggtgc agggcaatta
      541 catcatgccg cccatgccac ccatgccgcc cataccgcca gcagcgccta agtcagctgc
      601 atcatttttc ggcaggtcgg taaccatgca ctcggtggtg atcatcaggc cagccacaga
      661 agccgcgtac tgcagagcag aacgggttac tttggttggg tccaggatac ccatgtcgat
      721 catgttgccg tattcttcgg ttgctgcgtt gtaaccgtag ttgccgtcgc cgcctttaac
      781 ggtgttagca acaacagacg gttcttcgcc gcagttcagg acgatctgac gcagcggagc
      841 ttccattgca cgcagtgcaa ctttgatacc cacgttctgg tcttcgttct gaccacgcag
      901 gtcagccagt ttagacgcta cgcggatcag cgcaacacca ccaccagcaa ccacgccttc
      961 ttctaccgca gcacgggtcg cgtgcagggc gtcttcaacg cgtgcttttt tctctttcat
     1021 ttcaacttcg gtagcagcac ctactttgat aactgcaacg ccgcctgcca gtttcgctac
     1081 gcgttcctgc agtttttcac ggtcgtagtc agaagttgct tcttcaatct gctgacggat
     1141 ctgagcaaca cggccctgga ttgcagcttc ttcacccacg ccatcgatga tagtggtggt
     1201 gtctttgttg atcacaacac gtttagcctg acccaggtct tccagggttg ctttttccag
     1261 ctccataccg atctcttcag agattacggt accgccagtc agggttgcga tatcctgcag
     1321 catagcttta cgacgatcgc cgaagcccgg agctttaacc gcagcaactt tcacgatgcc
     1381 acgcatggtg ttaacaacca gagttgccag cgcttcgcct tcaacatctt cagcgatgat
     1441 cagcagcggt ttgcctgctt tggcaacagc ttccagaacc ggcagcattt cgcggatgtt
     1501 agagattttc ttgtcagcca gcaggatgaa cgggctttcc agttctactg cgccagtttc
     1561 cggcttgttg atgaagtaag gagacaggta gccacggtcg aactgcatac cttcaaccac
     1621 gtccagttcg tcctgcagac cggtaccgtc ttcaacggtg ataacgcctt ctttaccgac
     1681 tttgtccatc gcttcagcga tcagtttacc tacggtttcg tcggagttag cggagatggt
     1741 accaacctga gcaatcgctt tagagtcaga gcacggtacg gacagcgctt tcagttcttc
     1801 aactgcagcg gtaacggctt tgtcgatacc acgtttcagg tccatcgggt tcatgcccgc
     1861 agcaacagct ttcagaccct cagtgatgat agcctgagcc agtacagttg cagtggtggt
     1921 accgtcgcct gcagcgtcgt tcgctttaga ggcaacttct ttcaccatct gcgcacccat
     1981 attttcgaac ttgtcttcca gttcgatttc acgagcaacg gaaacaccat ctttggtgat
     2041 ggtcggtgca ccgaaagatt tatccagaac tacgttacgg cctttcggac cgagggtaac
     2101 tttcactgca tctgccagta cgtttacgcc gcgcagcatt ttcacacgag cgtcgttacc
     2161 gaattttacg tctttagctg ccattatctt tattccttaa attcgtatgt tcagtgtcgt
     2221 gcgaggatta cgcttcaaca attgccagaa tgtcgctttc ggacatgatc aacacttctt
     2281 cattgtcgat cttctcagat ttcacaccgt agccatcgtt gaaaataacg atgtcgccaa
     2341 ctttcacatc cagcggcttc acttcgccat tttcaaggat acggccattg ccgacagcca
     2401 gcacttcgcc gcgggtggat ttagccgctg cagagccggt cagaacgatg ccgccagcag
     2461 atttagtttc aacttcttta cgcttgacga tcacgcgatc atgcaatgga cgaatattca
     2521 ttgataactc tcctttgaga aagtccgtat ctgttatggg tgacgccgga gcttacgtgg
     2581 tttcccggct ggtgaccaga gaaatgggga tgaggattcg ccccttcaag ggggaaaaag
     2641 aaaaaaaatt ctgatcagca caaaatcggg tgaaaaccct gattcacctc acatttcatc
     2701 gcaatttctt catcgccgtc ataagcgaat ctgattgtgc taccatcgaa aatctacgca
     2761 gttgcccaaa atttgggcgc aatcggatat caacccatga gtggactcaa acaagaactg
     2821 gggctggccc aaggcattgg cctgctatcg acgtcattat taggcactgg cgtgtttgcc
     2881 gttcctgcgt tagctgcgct ggtagcgggc aataacagcc tgtgggcgtg gcccgttttg
     2941 attatcttag tgttcccgat tgcgattgtg tttgcgattc tgggtcgcca ctatcccagc
     3001 gcaggcggtg tcgcgcactt cgtcggtatg gcgtttggtt cgcgacttga gcgagtcacc
     3061 ggctggttgt ttttatcggt cattcccgtg ggtttgcctg ccgcgctaca aattgccgcc
     3121 gggttcggcc aggcaatgtt tggctggcat agctggcaac tgttgttggc agaactcggt
     3181 acgctggcgc tggtgtggta tatcggtact cgcggtgcca gttccagtgc taatctacaa
     3241 accgttattg ccggacttat cgtcgcgctg attgtcgcta tctggtgggc gggcgatatc
     3301 aaacctgcga atatcccctt cccggcacct ggtaatatcg aacttaccgg gttatttgct
     3361 gcgttatcag tgatgttctg gtgttttgtc ggtctggaag catttgccca tcttgcctcg
     3421 gaatttaaaa atccagagcg tgattttcct cgcgcattga tgattggtct gctgctggca
     3481 ggattagtct actggggctg tacggtagtc gtcttacact tcgacgccta tggtgaaaaa
     3541 atggcggcgg cagcatcgct tccaaaaatt gtagtgcagt tgttcggtgt aggagcgtta
     3601 tggattgcct gcgtgattgg ctatctggcc tgctttgcca gtctcaacat ttatatacag
     3661 agcttcgccc gcctggtctg gtcgcaggcg caacataatc ctgaccacta cctggcacgc
     3721 ctctcttctc gccatatccc gaataatgcc ctcaatgcgg tgctcggctg ctgtgtggtg
     3781 agcacgttgg tgattcatgc tttagagatc aatctggacg ctcttattat ttatgccaat
     3841 ggcatcttta ttatgattta tctgttatgc atgctggcag gctgtaaatt attgcaagga
     3901 cgttatcgac tactggcggt ggttggcggg ctgttatgcg ttctgttact ggcaatggtc
     3961 ggctggaaaa gtctctatgc gctgatcatg ctggcggggt tatggctgtt gctgccaaaa
     4021 cgaaaaacgc cggaaaatgg cataaccaca taatccggcg tttcgacatt aatcctggcg
     4081 atcgtcttta tgatcaaggc ggtcgcgctc atcatccttt cgctggtact caccatcaaa
     4141 agtattaccg ccaccggtcc cggcgctaaa accgccgcct ggcatacgag aaaagcgcaa
     4201 atgcggcatc aacttcactg tcagatgctt ttgcaccggc ggtaataaaa gcagaagacc
     4261 gaggaagtcg gtgaaaaagc ccggtaataa aagcagcaaa ccggcaatga tcagcgaaac
     4321 acttttaatc atctccgccg ctgggttttc acccgccgcc attttttgct gcatcagcac
     4381 gaaattctta aagccctggt tacgtaccag tgacatcccg ataactgacg tgaatataac
     4441 cagcacgagg gtcagcaata cccccaatac atgggcaacc tgaataaaga ttgaaatctc
     4501 aatatagaca taaaggaaaa tggcaataaa aggtaaccag cgcaaaggtt tctcctgtaa
     4561 tagcagccgg taaaacccgg ctacctgaat gggttgcgaa tcgcgttcag cttatattgt
     4621 ggtcattagc aaaatttcaa gatgtttgcg caactatttt tggtagtaat cccaaagcgg
     4681 tgatctattt cacaaattaa taattaaggg gtaaaaaccg acacttaaag tgatccagat
     4741 tacggtagaa atcctcaagc agcatatgat ctcgggtatt cggtcgatgc aggggataat
     4801 cgtcggtcga aaaacattcg aaaccacata tattctgtgt gtttaaagca aatcattggc
     4861 agcttgaaaa agaaggttca catgtcaaac aacattcgta tcgaagaaga tctgttgggt
     4921 accagggaag ttccagctga tgcctactat ggtgttcaca ctctgagagc gattgaaaac
     4981 ttctatatca gcaacaacaa aatcagtgat attcctgaat ttgttcgcgg tatggtaatg
     5041 gttaaaaaag ccgcagctat ggcaaacaaa gagctgcaaa ccattcctaa aagtgtagcg
     5101 aatgccatca ttgccgcatg tgatgaagtc ctgaacaacg gaaaatgcat ggatcagttc
     5161 ccggtagacg tctaccaggg cggcgcaggt acttccgtaa acatgaacac caacgaagtg
     5221 ctggccaata tcggtctgga actgatgggt caccagaaag gtgaatatca gtacctgaac
     5281 ccgaacgacc atgttaacaa atgtcagtcc actaacgacg cctacccgac cggtttccgt
     5341 atcgcagttt actcttccct gattaagctg gtagatgcga ttaaccaact gcgtgaaggc
     5401 tttgaacgta aagctgtcga attccaggac atcctgaaaa tgggtcgtac ccagctgcag
     5461 gacgcagtac cgatgaccct cggtcaggaa ttccgcgctt tcagcatcct gctgaaagaa
     5521 gaagtgaaaa atatccaacg taccgctgaa ctgctgctgg aagttaacct tggcgcaaca
     5581 gcaatcggta ctggtctgaa cacgccgaaa gagtactctc cgctggcagt gaaaaaactg
     5641 gctgaagtca ctggcttccc atgcgtaccg gctgaagacc tgatcgaagc gacctctgac
     5701 tgcggcgctt atgttatggt tcacggcgcg ctgaaacgcc tggctgtgaa gatgtccaaa
     5761 atctgtaacg acctgcgctt gctctcttct ggtccacgtg ccggcctgaa cgagatcaac
     5821 ctgccggaac tgcaggcggg ctcttccatc atgccagcta aagtaaaccc ggttgttccg
     5881 gaagtggtta accaggtatg cttcaaagtc atcggtaacg acaccactgt taccatggca
     5941 gcagaagcag gtcagctgca gttgaacgtt atggagccgg tcattggcca ggccatgttc
     6001 gaatccgttc acattctgac caacgcttgc tacaacctgc tggaaaaatg cattaacggc
     6061 atcactgcta acaaagaagt gtgcgaaggt tacgtttaca actctatcgg tatcgttact
     6121 tacctgaacc cgttcatcgg tcaccacaac ggtgacatcg tgggtaaaat ctgtgccgaa
     6181 accggtaaga gtgtacgtga agtcgttctg gaacgcggtc tgttgactga agcggaactt
     6241 gacgatattt tctccgtaca gaatctgatg cacccggctt acaaagcaaa acgctatact
     6301 gatgaaagcg aacagtaatc gtacagggta gtacaaataa agaaggcacg tcagatgacg
     6361 tgcctttttt cttgtgagca gtaacttaaa aataacaacc taatatcaac ttgttaaaaa
     6421 acaaggaagg ctaatatgct agttgtagaa ctcatcatag ttttgctggc gatcttcttg
     6481 ggcgccagat tggggggaat aggtattggt tttgcaggcg gattgggggt gctggttctt
     6541 gccgctattg gcgttaaacc cggtaacatc ccgttcgatg tcatttccat tatcatggcg
     6601 gttatcgccg ctatttctgc catgcaggtt gctggcggtc tggactatct ggttcatcag
     6661 acagaaaagc tgctgcgccg taacccgaaa tacatcacga tcctcgcacc gatcgtgacc
     6721 tatttcctga ctatctttgc tggtactggc aacatatctc tggcgacact gccagttatc
     6781 gctgaagttg cgaaggaaca aggcgtcaaa ccttgccgtc cgctgtctac tgcagtggta
     6841 tccgcgcaga ttgcgatcac cgcatcgcca atctcagcgg cagtggttta catgtcttcc
     6901 gtgatggaag gtcatggcat cagctacctc catctgctct ccgtggtcat cccgtccacc
     6961 ctgctggcgg ttctggtgat gtcctttctg gtcactatgc tgttcaactc caaactctct
     7021 gacgatccga tttatcgcaa gcgtctggaa gagggcctgg ttgaactgcg cggtgaaaag
     7081 cagattgaaa tcaaatccgg tgcaaaaacg tccgtctggc tgttcctgct gggcgtagtt
     7141 ggcgtggtta tctatgcaat catcaacagc ccaagcatgg gtctggttga aaaaccgctg
     7201 atgaacacca ccaacgcaat cctgatcatc atgctcagcg ttgcaactct gaccaccgtt
     7261 atctgtaaag tcgataccga caacatcctc aactccagca ccttcaaagc aggtatgagc
     7321 gcctgtattt gtatcctggg tgttgcgtgg ctgggcgata ctttcgtttc caacaacatc
     7381 gactggatca aagataccgc tggtgaagtg attcagggtc atccgtggct gctggccgtc
     7441 atcttcttct ttgcttctgc tctgctgtac tctcaggctg caaccgcaaa agcactgatg
     7501 ccgatggccc tggcactgaa cgtttcaccg ctgaccgctg ttgcttcttt cgctgcggtg
     7561 tctggtctgt tcattctgcc gacctacccg acgctggttg ctgcggtaca gatggatgac
     7621 acgggtacaa cccgtatcgg taaattcgtc ttcaaccatc cgttcttcat cccgggtact
     7681 ctgggtgttg ccctggccgt ttgcttcggc ttcgtgctgg gtagcttcat gctgtaatga
     7741 ccaatcgcgg ggcgttcacg ccccgctttc tttcccgccg actaacatcc ttcccccgtc
     7801 cgttgtatag tgacctctct cttgcggttc catctgttct tgcgaggtgt ttatgcttga
     7861 tgaagaaagt tcgaataccg cgtctgtcgt ggtgctatgt accgcaccgg atgaagcgac
     7921 agcccaggat ttagccgcca aagtgctggc ggaaaaactg gcggcctgcg cgaccttgat
     7981 ccccggcgct acctctctct attactggga aggtaagctg gagcaagaat acgaagtgca
     8041 gatgatttta aaaactaccg tatctcacca gcaggcactg ctggaatgcc tgaagtctca
     8101 tcatccatat caaaccccgg aacttctggt tttacctgtt acacacggag acacagatta
     8161 cctctcatgg ctcaacgcat ctttacgctg atcctgctac tttgcagcac ttccgttttt
     8221 gccggattat tcgacgcgcc gggacgttca cattttgtcc ccgcggatca agcctttgct
     8281 tttgattttc agcaaaacca acatgacctg aatctgacct ggcagatcaa agacggttac
     8341 tacctctacc gtaaacagat ccgcattacg ccggaacacg cgaaaattgc cgacgtgcag
     8401 ctgccgcaag gcgtctggca tgaagatgag ttttacggca aaagcgagat ttaccgcgat
     8461 cggctgacgc ttcccgtaac catcaaccag gcgagtgcgg gagcgacgtt aactgtcacc
     8521 taccagggct gtgctgatgc cggtttctgt tatccgccag aaaccaaaac cgttccgtta
     8581 agcgaagtgg tagccaacaa cgcagcgtca cagcctgtgt ctgttccgca gcaagagcag
     8641 cacaccgcgc aattgccctt ttccgcgctc tgggcgttgt tgatcggtat tggtatcgcc
     8701 tttacgccat gcgtgctgcc aatgtaccca ctgatttctg gcatcgtgct gggtggtaaa
     8761 cagcggctct ccactgccag agcattgttg ctgaccttta tttatgtgca ggggatggca
     8821 ctgacttaca cggcgctggg tctggtagtt gccgccgcag ggttacagtt ccaggcggcg
     8881 ctacagcacc catacgtgct cattggcctc gccatcgtct ttaccttgct ggcgatgtca
     8941 atgtttggct tgtttactct acaactcccc tcttcgctgc aaacacgtct cacgttgatg
     9001 agcaatcgcc aacagggcgg ctcacctggc ggtgtgtttg ttatgggggc gattgccgga
     9061 ctgatctgtt caccatgcac caccgcaccg cttagcgcga ttctgctgta tatcgcccaa
     9121 agcgggaaca tgtggctggg cggcggcacg ctttatctct atgcgttggg catgggcctg
     9181 ccgctgatgc taattaccgt ctttggtaac cgcttgctgc cgaaaagcgg cccgtggatg
     9241 gaacaagtca aaaccgcgtt tggttttgtg atcctcgcac tgccggtctt cctgctggag
     9301 cgagtgattg gtgatgtatg gggattacgc ttgtggtcgg cgctgggtgt cgcattcttt
     9361 ggctgggcct ttatcaccag cctacaggct aaacgcggct ggatgcgcgt ggtgcaaata
     9421 atcctgctgg cagcggcatt ggttagcgtg cgcccacttc aggattgggc atttggtgcg
     9481 acgcataccg cgcaaactca gacgcatctc aactttacac aaatcaaaac ggtagatgag
     9541 ttaaatcagg cgctcgttga agtcaaaggc aaaccggtga tgttagatct ttatgccgac
     9601 tggtgcgtcg cctgtaaaga gtttgagaaa tacaccttca gcgacccgca ggtgcaaaaa
     9661 gcgttagcag acacggtctt acttcaggcc aacgtcacgg ccaacgacgc acaagatgtg
     9721 gcactgttaa agcatcttaa tgtccttggc ctaccgacaa ttctcttttt tgacggacaa
     9781 ggccaggagc atccacaagc acgcgtcacg ggctttatgg atgctgaaac cttcagcgca
     9841 catttgcgcg atcgccaacc gtgaacgaca cttgcagtgg gaaagacgga ggaaaatagc
     9901 gtgcaacgtg aagatgtact gggagaagcc ctgaaattat tagaattaca agggattgcc
     9961 aacaccacgc tggagatggt tgctgaacgt gtggattatc cactggacga gctacgccgc
    10021 ttctggccag acaaagaggc aatcctctac gatgcgctgc gttatcttag ccaacagata
    10081 gatgtctggc gtcgtcagct gatgctggac gaaacgcaaa ccgccgaaca aaagctgctg
    10141 gcacgttatc aggcgttatc ggagtgcgtt aaaaacaacc gctatccggg ctgtctattt
    10201 atcgctgcct gtacgtttta tcccgatcct ggccacccta ttcatcaact ggccgatcag
    10261 caaaaaagcg cggcctacga tttcacccac gaactgttaa ccacgctgga agttgacgat
    10321 ccggcgatgg tagcaaagca gatggaactg gtgctggaag gctgtttaag ccgaatgctg
    10381 gtgaatcgta gccaggcgga tgtcgacact gcacatcggc tggcggaaga tatcctgcgc
    10441 ttcgcccgct gccgccaggg tggtgcattg acctgacaga aacacagaaa agaagcgatt
    10501 tgccgcaatc ttaagcagtt gaatcgcttt tactgaaatt aggttgacga gatgtgcaga
    10561 ttacggttta atgcgccccg ttgcccggat agctcagtcg gtagagcagg ggattgaaaa
    10621 tccccgtgtc cttggttcga ttccgagtcc gggcaccaaa ttcatatcaa cggacctcca
    10681 cggaggtccg tttttcgttt cagaacacca tgatttaagt gttcttcc
//
LOCUS       DAZKHF020000154        10652 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2201S31241
            SAMN43267800-rid25106413.denovo.155, whole genome shotgun sequence.
ACCESSION   DAZKHF020000154 DAZKHF020000000
VERSION     DAZKHF020000154.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267800
            Sequence Read Archive: SRR30307263
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 10652)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10652)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 10652)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350581.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350581.2
            Genome Coverage       :: 151x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:21:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,652
            CDSs (total)                      :: 5,557
            Genes (coding)                    :: 5,320
            CDSs (with protein)               :: 5,320
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 237
            CDSs (without protein)            :: 237
            Pseudo Genes (ambiguous residues) :: 0 of 237
            Pseudo Genes (frameshifted)       :: 102 of 237
            Pseudo Genes (incomplete)         :: 130 of 237
            Pseudo Genes (internal stop)      :: 52 of 237
            Pseudo Genes (multiple problems)  :: 41 of 237
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10652
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267800-rid25106413.denovo.155"
                     /serotype="O 26:H11"
                     /isolate="2201S31241"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-01"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            17..730
                     /gene="yghS"
                     /locus_tag="ACHOSC_RS15940"
                     /old_locus_tag="pgaptmp_003188"
     CDS             17..730
                     /gene="yghS"
                     /locus_tag="ACHOSC_RS15940"
                     /old_locus_tag="pgaptmp_003188"
                     /EC_number="2.7.4.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004798 - thymidylate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006233 - dTDP biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0006235 - dTTP biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTMP kinase"
                     /protein_id="HGU4549084.1"
                     /translation="MNQRNMSIINSTPVRVIAIVGCDGSGKSTLTASLVNELAARMPT
                     ERIYLGQSSGRIGEWISQLPVIGAPFGRYLRSKAAHVHEKPSTPPGNITALVIYLLSC
                     WRAYKFRKMLCKSQQGFLLITDRYPQVEVPGFRFDGPQLAKTTGGNGWIKMLRQRELK
                     LYQWMASYLPVLLIRLGIDEQTAFARKPDHQLAALQEKIAVTPQLTFNGAKILELDGR
                     HPADEILQASLRAIHAALS"
     gene            762..1520
                     /locus_tag="ACHOSC_RS15945"
                     /old_locus_tag="pgaptmp_003189"
     CDS             762..1520
                     /locus_tag="ACHOSC_RS15945"
                     /old_locus_tag="pgaptmp_003189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4549085.1"
                     /translation="MDALQTQTVNSTTAPQPNYIPGLIAVVGCDGTGKSTLTTDLVKS
                     LQQHWQTERRYLGLLSGEDGDKIKRLPLVGVWLERRLAAKSSKTQSMKTKSPALWAAV
                     IMYCFSLRRMANLRKVQRLAQSGVLVVSDRFPQAEISGFYYDGPGIGVERATGKISMF
                     LAQRERRLYQQMAQYRPELIIRLGIDIETAISRKPDHDYAELQDKIGVMSTIGYNGTK
                     ILEIDSRAPYSEVLEQAQKAVSLVAIVSDRRSLT"
     gene            1566..2915
                     /locus_tag="ACHOSC_RS15950"
                     /old_locus_tag="pgaptmp_003190"
     CDS             1566..2915
                     /locus_tag="ACHOSC_RS15950"
                     /old_locus_tag="pgaptmp_003190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide biosynthesis protein"
                     /protein_id="HGU4549086.1"
                     /translation="MAGFNIKHWFADGAFRTIIRNSAWLGSSNVVSALLGLLALSCAG
                     KGMTPAMFGVLVIVQSYAKSISDFIKFQTWQLVVQYGTPALTNNNPQQFRNVVSFSFS
                     LDIVSGAVAIVGGIALLPFLSHSLGLDDQSFWLAALYCTLIPSMASSTPTGILRAVDR
                     FDLIAVQQATKPFLRAAGSVVAWYFDFGFAGFVIAWYVSNLVGGTMYWWFAARELRRR
                     NIHNAFKLNLFESARHIKGAWSFVWSTNIAHSIWSARNSCSTVLVGIVLGPAAAGLFK
                     IAMTFFDAAGTPAGLLGKSFYPEVMRLDPRTTRPWLLGVKSGLLAGGIGILVALAVFI
                     VGKPLISLVFGVKYLEAYDLIQVMLGAIVISMLGFPQESLLLMAGKQRAFLVAQTIAS
                     IGYIVLLFMFCHLFGVLGAAFAYFGGQCLDVALSLIPTLKAFFQRHSLLYNAAGEKS"
     gene            2915..3739
                     /locus_tag="ACHOSC_RS15955"
                     /old_locus_tag="pgaptmp_003191"
     CDS             2915..3739
                     /locus_tag="ACHOSC_RS15955"
                     /old_locus_tag="pgaptmp_003191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4549087.1"
                     /translation="MMRKYFPLEASERLFVAIEEDDVVDAQVSLPPTIALSCTTEIIH
                     DNYALCLQFWLNGVDRQELLRLVRKQAKGDELTADERKQFKYMRARYKHLRFAQRLYL
                     KKHQAGFLFGKTTVFLGRFQDGFRNGKKNIVSYYGNLLRIYLSSPVWSLVNYSLRHSQ
                     LESVSSFIAYRQKQMHTLKEIIAKPRLTGREFHDVRKIISQQVSYYDTLRSLDPENKE
                     ALQISRFLAAINGLMGDKHDDMVADDMENRQSYDAPLALDSDIRQRLELLISRFPL"
     gene            3751..4311
                     /locus_tag="ACHOSC_RS15960"
                     /old_locus_tag="pgaptmp_003192"
     CDS             3751..4311
                     /locus_tag="ACHOSC_RS15960"
                     /old_locus_tag="pgaptmp_003192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708799.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtfJ family protein"
                     /protein_id="HGU4549088.1"
                     /translation="MSSRLIIALIIMLLAPGVQAHNFVTGKTVTPVYIQEGGELLLNS
                     DDEIHYQKWNSTQLAGKVRIIQYIAGRKSAKKKNSLLIKAVEAANFPQDRFQPTTIVN
                     TDDAIFGTGYFVVGKIEKNKRRYPWAQFIIDGNGQGRVAWRLPEQSSTILVLNKAGQI
                     QWAKDGSLTPEEVDHVIALAQKLINE"
     gene            4342..5412
                     /gene="lptF"
                     /locus_tag="ACHOSC_RS15965"
                     /old_locus_tag="pgaptmp_003193"
     CDS             4342..5412
                     /gene="lptF"
                     /locus_tag="ACHOSC_RS15965"
                     /old_locus_tag="pgaptmp_003193"
                     /EC_number="7.5.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0015221 - lipopolysaccharide transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter permease LptF"
                     /protein_id="HGU4549089.1"
                     /translation="MKLVEHYIMRGTRRLVLIIVGFLIFIFASYSAQRYLTEAANGTL
                     ALDVVLDIVFYKVLIALEMLLPVGLYVSVGVTLGQMYTDSEITAISAAGGSPGRLYKA
                     VLYLAIPLSIFVTLLSMYGRPWAYTQIYQLEQQSQSELDVRQLRAKKFNTNDNGRMIL
                     SQTVDQDNNRLTDALIYTSTANRTRIFRARSVDVVDPSPEKPTVMLHNGTAYLLDHQG
                     RDDNEQIYRNLQLHLNPLDQSPNVKRKAKSVTELARSAFPADHAELQWRQSRGLTALL
                     MALLAISLSRVKPRQGRFSTLLPLTLLFIAIFYGGDVCRTLVANGAIPLIPGLWLVPG
                     LMLMGLLMLVARDFSLLQKFSR"
     gene            5409..6488
                     /gene="lptG"
                     /locus_tag="ACHOSC_RS15970"
                     /old_locus_tag="pgaptmp_003194"
     CDS             5409..6488
                     /gene="lptG"
                     /locus_tag="ACHOSC_RS15970"
                     /old_locus_tag="pgaptmp_003194"
                     /EC_number="7.5.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0015221 - lipopolysaccharide transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter permease LptG"
                     /protein_id="HGU4549090.1"
                     /translation="MNVFSRYLIRHLFLGFAAAAGLLLPLFTTFNLINELDDVSPGGY
                     RWTQAVLVVLMTLPRTLVELSPFIALLGGIVGLGQLSKNSELTAIRSTGFSIFRIALV
                     VLVAGILWTVSLGAIDEWVASPLQQQALQIKSTATALGEDDDITGNMLWARRGNEFVT
                     VKSLNEQGQPVGVEIFHYRDDLSLESYIYARSATIKDDKTWVLHGVNHKKWLNGKETL
                     ETLDNLAWQSAFTSMNLEELSMPGNTFSVRQLNHYIHYLQETGQPSSEYRLALWEKLG
                     QPILTLAMILLAVPFTFTAPRSPGMGSRLAVGVIVGLLTWISYQIMVNLGLLFALSAP
                     VTALGLPVAFVLVALSLVYWYDRQH"
     gene            complement(6524..7696)
                     /locus_tag="ACHOSC_RS15975"
                     /old_locus_tag="pgaptmp_003195"
     CDS             complement(6524..7696)
                     /locus_tag="ACHOSC_RS15975"
                     /old_locus_tag="pgaptmp_003195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020239167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HGU4549091.1"
                     /translation="MGLYDKYARLAGERLQFSDNGLTPFGTCIDEVYSATEGRIGNKK
                     VILAGTNNYLGLTFNHDAISEGQAALAAQGTGTTGSRMANGSYAPHLALEKEIAEFFN
                     RPTAIVFSTGYTANLGVISALADHNAVVLLDADSHASIYDACSLGGAEIIRFRHNDAK
                     DLERRMVRLGERAKEAIIIVEGIYSMLGDVAPLAEIVDIKRRLGGYLIVDEAHSFGVL
                     GATGRGLAEAVGVEDDVDIIVGTFSKSLASIGGFAVGSEAMEVLRYSSRPYIFTASPS
                     PSCIATVRSSLRTIATQPELRQKLMDNANHLYDGLQKLGYELSSHISPVVPVIIGSKE
                     EGLRIWRELISLGVYVNLILPPAAPAGITLLRCSVNAAHSREQIDAIIQAFATLKQ"
     gene            complement(7696..7944)
                     /locus_tag="ACHOSC_RS15980"
                     /old_locus_tag="pgaptmp_003196"
     CDS             complement(7696..7944)
                     /locus_tag="ACHOSC_RS15980"
                     /old_locus_tag="pgaptmp_003196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000036 - acyl carrier activity [Evidence
                     IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HGU4549092.1"
                     /translation="MVNREIVMDYILSCLQDLVENGVEIKPDSDLVNDLGLESIKVMD
                     LLMMLEDRFDISIPINILLDVKTPAQLMETLLPWLENK"
     gene            complement(7976..8890)
                     /locus_tag="ACHOSC_RS15985"
                     /old_locus_tag="pgaptmp_003197"
     CDS             complement(7976..8890)
                     /locus_tag="ACHOSC_RS15985"
                     /old_locus_tag="pgaptmp_003197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001481799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HGU4549093.1"
                     /translation="MNQTVAVTGATGFIGKYIIDNLLARGFHVRALTRTARAHVNDNL
                     TWVRGSLEDTHSLSKLVAGASAVVHCAGQVRGHKEEIFTRCNVDGSLRLMQAAKESCF
                     CQRFLFISSLAARHPELSWYANSKHIAEQRLTAMADEITLGVFRPTAVYGPGDKELKP
                     LFDWMLRGLLPRLGAPDTQLSFLHVTDFAQAVGQWLSAETVQTQTYELCDSVAGGYDW
                     QRIQQLAANVRCGSVRMVGIPLPVLTCLADISTALSRLAGKEPMLTRSKIRELTHADW
                     SASNNRISEDINWFPGISLEHALRNGLF"
     gene            complement(8887..10575)
                     /locus_tag="ACHOSC_RS15990"
                     /old_locus_tag="pgaptmp_003198"
     CDS             complement(8887..10575)
                     /locus_tag="ACHOSC_RS15990"
                     /old_locus_tag="pgaptmp_003198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708792.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acyl-AMP ligase"
                     /protein_id="HGU4549094.1"
                     /translation="MRYADFPTLVDALDYAARSSAGMNFYDRRCQLEDQLEYQTLKTR
                     AEAGAKRLLSLNLKKGERVALIAETSSGFVEAFFACQYAGLVAVPLAIPMGVGQRDSW
                     SAKLQGLLASCQPAAIITGDEWLPLVNAATHNNPELHVLSHAWFKALPEADVALQRPV
                     PNDIAYLQYTSGSTRFPRGVIITHREVMANLRAISHDGIKLRPGDRCVSWLPFYHDMG
                     LVGFLLPPVATQLSVDYLRTQDFAMRPLQWLKLISKNRGTVSVAPPFGYELCQRRVNE
                     KDLAELDLSCWRVAGIGAEPISAEQLHQFAECFRQVNFDNKTFMPCYGLAENALAVSF
                     SDEASGVVVNEVDRDILEYQGKAVAPGAETRAVSTFVNCGKALPEHGIEIRNEAGMPV
                     AERVVGHICISGPSLMSGYFGDQVSQDEIAATGWLDTGDLGYLLDGYLYVTGRIKDLI
                     IIRGRNIWPQDIEYIAEQEPEIHSGDAIAFVTAQEKIILQIQCRISDEERRGQLIHAL
                     AARIQSEFGVTAAIDLLPPHSIPRTSSGKPARAEAKKRYQKAYAASLNVQESLA"
BASE COUNT         2554 a         2660 c         2796 g         2642 t
ORIGIN      
        1 tactgccggg tttttcgtga atcaacgtaa tatgtcaata attaattcca cgccagtgcg
       61 tgttattgcc attgtaggat gtgatggttc aggtaaatcg accctcacgg caagcctggt
      121 aaatgaactg gcagcaagaa tgccaacaga acgcatttat ctcgggcaat cgtccgggcg
      181 aattggcgaa tggatttcac agctccctgt tattggcgca ccttttgggc gttatctgcg
      241 aagtaaagcg gcacatgtgc acgaaaagcc ctcaacaccg cctggcaata ttactgcgct
      301 ggttatctat ctgctttcct gctggcgggc gtacaagttt cgcaaaatgt tgtgtaaaag
      361 ccagcaaggc tttctgctca tcaccgaccg ctacccgcag gttgaagtgc cgggttttcg
      421 ctttgatggc ccgcaattgg caaaaaccac gggcggtaac ggttggataa aaatgttaag
      481 gcagcgcgag ctgaagctgt accaatggat ggcatcttat ttgcccgtat tgttgattcg
      541 tcttggcatt gatgaacaaa ccgcgtttgc gcgtaaacct gaccaccaac tggcagcgtt
      601 acaggaaaaa atcgccgtta cgccgcaact gacattcaat ggcgcaaaga tccttgaact
      661 ggatgggcga caccccgccg atgaaattct gcaagcgtca ctacgcgcaa ttcacgccgc
      721 gctttcctga tcaccaaaat ggatagggga tgatttaacg aatggatgca ctacaaactc
      781 aaactgttaa tagtacaacc gcgccgcagc ccaactacat tccggggctg attgcggtgg
      841 tcgggtgtga tggcaccggt aaatccacac tgaccaccga cctggtgaaa tcgctgcaac
      901 aacactggca aaccgagcgg cgctatctgg ggctgctctc cggcgaagac ggcgacaaaa
      961 tcaaacgatt gccgttggtt ggcgtctggc tggaacggcg actggcggcc aaatcctcga
     1021 aaacccaaag catgaaaacc aaatctccgg cgctatgggc ggcggtgatt atgtactgct
     1081 tctcgctgcg aagaatggcg aatctacgca aggttcagcg actggcgcaa agtggcgttc
     1141 tggtggtcag cgatcgcttc ccgcaggctg aaatttcggg cttttattat gatggaccgg
     1201 ggattggcgt cgaacgtgcg accgggaaaa tcagcatgtt tctggcgcag cgcgaacggc
     1261 gtttatacca acaaatggcg caatatcgcc cggaattaat tattcgcctg ggcattgata
     1321 ttgagactgc catctcccgc aagcctgacc atgactatgc cgagctgcag gacaaaatcg
     1381 gcgtcatgtc gacgattggc tataacggca caaaaattct tgagattgat tccagagcgc
     1441 cctacagcga agtgctggaa caggcacaga aggcggtttc tctggttgcc atcgtttctg
     1501 accgccgaag tttaacttaa ggctggcggg atacttttta ctatcaacga taaggtcagg
     1561 ctgaattggc gggttttaac atcaaacatt ggtttgcaga tggcgcgttt cgcaccatta
     1621 ttcgcaatag cgcctggtta ggctccagta atgtcgtgag cgccttgctg ggtctgttgg
     1681 cgctctcgtg tgccggtaaa gggatgacgc ccgccatgtt tggcgtactg gtgattgtgc
     1741 aatcgtacgc caagtcgatc agcgatttta ttaagtttca gacatggcaa ctggtggttc
     1801 agtacggaac gccagccttg accaacaata atccgcagca atttcgcaat gtcgtctcat
     1861 tttccttctc gctggatatc gtcagcggcg cggtggcgat tgtcggcggc attgccttac
     1921 tgccattcct ttcccattca ttaggtctgg atgaccaaag cttttggctg gcggcgctgt
     1981 attgcacgct cattccttca atggcttcct ccacgccgac cggcattctg cgtgcggtag
     2041 atcgcttcga tttaattgct gtacagcagg cgacgaaacc ttttctgcgc gcagcgggga
     2101 gcgtcgtagc ctggtatttt gactttggtt ttgcgggttt tgttattgcc tggtacgtgt
     2161 cgaatctggt tggcggcacc atgtactggt ggtttgcagc gcgcgaatta cgccgcagaa
     2221 atatccataa tgccttcaaa ttgaatctgt ttgagtctgc ccgacacatc aaaggcgcgt
     2281 ggagttttgt ctggtcaacc aacattgccc actccatctg gtcggcgcgt aactcgtgca
     2341 gcacagtgct ggtggggatc gtgttaggac ccgctgccgc cgggttattt aaaatcgcca
     2401 tgacattctt cgacgccgcc ggaacgccag cgggtttgct gggtaaaagt ttttacccag
     2461 aggtgatgcg tttagatccg cgcaccacca gaccgtggtt gttaggtgtg aagtccggtt
     2521 tactggcggg cggaatcggt attctggtgg cgctcgcggt gtttatcgtc ggcaagccgc
     2581 tcatttcgct ggtgtttggc gttaagtatc tcgaagcgta tgacctgatt caggtgatgc
     2641 tgggcgcaat tgtgatttcg atgctgggct tccctcagga gtcattgcta ttgatggcgg
     2701 gaaaacagcg cgcttttctc gtggcgcaaa ccatcgcctc aatcggctac atcgtattgc
     2761 tgttcatgtt ctgtcatctg tttggtgtgc tgggagcagc gtttgcttac ttcggtgggc
     2821 aatgtctgga cgtggcgctc tcgttaattc caaccttaaa ggcatttttt cagcgccatt
     2881 ccttgcttta taacgcagcc ggagagaaat cctgatgatg agaaaatatt tcccgcttga
     2941 ggcgagcgag cgtctatttg ttgcgattga agaagatgat gtcgttgatg cacaggttag
     3001 tttgccgccg actattgcac taagttgtac caccgaaatc attcatgata attacgcgtt
     3061 atgcctgcaa ttctggctca atggcgttga tcgtcaggaa ttactgcgtt tagttcgcaa
     3121 acaagccaaa ggcgatgagc tgactgctga tgagcgcaaa caatttaaat acatgcgagc
     3181 gcgctacaag cacctgcgtt ttgcgcaacg gctgtattta aagaagcatc aggccggatt
     3241 tttattcggc aaaacgaccg tttttctggg gcgttttcag gacggctttc gtaacggtaa
     3301 gaaaaatatt gtgtcgtatt acggcaacct gttacggatt tatttaagct ctccggtctg
     3361 gtcgttggtg aactattcat tacgccatag ccagttagaa agcgtaagca gttttattgc
     3421 ctatcggcaa aagcaaatgc ataccctgaa agagattatt gccaaaccgc gactaaccgg
     3481 cagggaattt catgatgtgc gaaaaatcat cagccaacag gtttcttatt acgacacctt
     3541 aagatcgctt gatcctgaaa ataaagaggc gttacagatt tcccgtttcc tcgccgccat
     3601 taatggcctg atgggagata aacacgacga catggtggcg gatgatatgg aaaaccgcca
     3661 gtcgtatgat gcaccgttgg cgctggatag cgatattcgt cagcgcttag agctgctgat
     3721 ttcgcgtttt ccgctatagc cggagcaatg atgtcatctc gtttgattat tgcgttgatt
     3781 atcatgctct tagccccggg tgtgcaggca cacaatttcg tcactggcaa aacggttacc
     3841 cctgtttata tccaggaagg cggcgagctg cttctgaata gcgacgacga aattcactac
     3901 caaaagtgga acagcacaca attagcagga aaagtccgca ttattcagta tatcgccggg
     3961 cgtaagtcgg cgaagaaaaa gaactcactg ttaattaaag cggttgaagc ggcaaacttt
     4021 ccgcaggatc gttttcagcc caccaccatt gtgaacaccg atgatgccat ctttggtact
     4081 ggctattttg ttgttggcaa aatcgaaaaa aataaacgcc gctatccgtg ggcgcaattc
     4141 attattgacg gcaatggaca gggacgcgtt gcctggcgct tgccggagca gagttcaacc
     4201 attctggtgc tgaataaagc cgggcaaatt cagtgggcaa aggacggcag cctgacgccg
     4261 gaagaggtag atcatgtcat tgcgctggcg cagaaactga tcaatgaata aaaaattcaa
     4321 aggattgagg gcgttagtga gatgaagctg gttgaacact acatcatgcg tggtacgcgc
     4381 cgtctggtgc tgattatcgt cggttttctg atctttattt tcgccagcta ctccgcacag
     4441 cgttatctga ccgaagcggc aaatgggaca ttagctcttg atgttgtgct ggatatcgtt
     4501 ttctacaaag tgctgattgc actggagatg ttgttacctg ttggtctgta tgtgtcagtt
     4561 ggcgtgacgc tagggcagat gtataccgac tcggaaatta ccgctatctc tgcggcgggg
     4621 ggcagtccgg gacgcttgta caaagccgtt ctttatctgg cgataccgct aagtattttt
     4681 gtcacccttc tgtcgatgta tggtcgtccg tgggcttata cgcagattta tcaactggag
     4741 cagcagtcgc agtcggagct ggatgttcgc cagttgcggg caaagaaatt taacactaac
     4801 gataacggac gaatgatcct ttcgcagacg gttgatcagg ataataatcg cctgactgac
     4861 gcgctgattt atacttctac tgccaatcga acccgcattt tccgcgcccg ttcggttgat
     4921 gtggttgacc catcacccga gaaaccgacc gttatgttgc acaacggcac tgcctatctt
     4981 ctcgatcatc aggggcgtga cgacaacgaa cagatctacc gtaatctgca attacatctg
     5041 aatccgctgg atcaaagccc taacgtcaaa cgcaaagcaa aatcggtcac ggagctggcg
     5101 cgctccgcct ttcctgccga tcatgccgaa ctgcaatggc gacaaagccg tggcctgaca
     5161 gcattgttga tggcgctgct ggccatttca ttaagtcggg taaaaccgcg gcaagggcga
     5221 ttttcaacat tattgccact gacgttgctg tttattgcca ttttttatgg cggcgacgtc
     5281 tgccgtacgc tggtggctaa cggcgcgatt cccctcattc ctggtttgtg gttagtaccc
     5341 ggactcatgc taatgggcct gctgatgctg gtcgcacgcg acttctcttt gctgcagaaa
     5401 ttttcccgat gaatgttttc agtcgctatt taatccgtca tctctttctc ggttttgccg
     5461 ccgccgcagg gctattgctg ccgcttttta ccaccttcaa cctgattaac gaactggatg
     5521 atgtcagccc gggcggttat cgctggactc aggcggtgct ggtggtgcta atgaccttac
     5581 cgcgcacact ggtcgaactt tcgccgttta tcgcgttatt gggtggtatt gttggactgg
     5641 ggcagttatc gaaaaacagt gagcttaccg ccattcgcag cacggggttt tctatcttcc
     5701 gtattgcact ggtggtgctg gttgccggga tattgtggac tgtttcgtta ggcgcgatag
     5761 atgagtgggt ggcgtcgcca ttgcagcaac aggcgttgca aatcaaatcg accgccaccg
     5821 cgttggggga ggacgatgac attaccggca atatgctgtg ggccaggcgt ggtaatgaat
     5881 ttgtgacggt gaaatcgctg aacgagcagg gccagccggt gggcgtggag atatttcatt
     5941 atcgcgacga tctctcgctt gaatcctaca tttatgcacg cagtgccacc attaaagacg
     6001 acaaaacgtg ggtcctgcat ggtgtgaatc ataaaaaatg gctgaatgga aaagaaacgc
     6061 tggaaacatt agataatctt gcctggcaat cggccttcac cagtatgaat cttgaagagt
     6121 tatcaatgcc ggggaatact ttttctgtcc gtcagcttaa tcattacatc cattatttgc
     6181 aggaaaccgg gcaacccagc agcgaatacc gccttgcact gtgggaaaaa ctggggcaac
     6241 ccatcctgac cctggcgatg attttgctgg ctgtgccgtt tacctttact gccccgcgtt
     6301 cgcctgggat gggtagccgt ctcgctgtag gcgtcatcgt gggcttactc acctggatca
     6361 gctatcaaat catggttaat ctgggattgc tatttgcgtt aagcgcacct gttaccgcgc
     6421 tcggtttacc ggtagcgttt gtgttggtgg cgttgagcct ggtgtattgg tatgacagac
     6481 aacattaaac ccgccgtggc gcaaacacca cggcgggctc acattactgc ttcagagtag
     6541 caaatgcctg aatgatggca tcgatttgct cacgactgtg ggcagcattt acgctacagc
     6601 gcagcagcgt gatgcctgca ggcgcggcag gcggcaggat aagattcaca tacacgccca
     6661 gcgagataag ctcgcgccag atgcgtaaac cttcctcttt cgaaccgatt attactggca
     6721 ctaccgggct aatatgtgaa cttaactcat aacccagttt ttgcagaccg tcatacagat
     6781 ggttggcgtt atccatcagc ttttgccgca gctctggttg tgtggcaata gttcttaacg
     6841 acgagcgcac ggtcgcaatg caggacggag aaggagaggc ggtaaaaata tacgggcggc
     6901 tgctgtagcg cagcacttcc atcgcttccg aaccgacagc aaaaccgcca atcgaggcca
     6961 gacttttact gaaagtacca acgataatat ccacatcatc ttcaacgccg acagcttcag
     7021 ccagtccgcg ccccgttgcg cctaacacgc caaacgaatg cgcttcatcg acaatcaaat
     7081 aaccaccgag acgacgtttg atatcaacaa tttccgccag cggcgcaacg tcaccgagca
     7141 tgctgtagat gccctcaacg atgataatcg cctctttcgc ccgttcaccg aggcgaacca
     7201 tgcgccgttc cagatcttta gcatcgttat ggcggaaacg aataatttcc gcgccaccga
     7261 gagaacaggc gtcataaata ctggcgtggc tgtcggcatc cagtaacacc acggcgttat
     7321 ggtcggcaag tgcgctgatg acgcctaaat tagcggtata accggttgaa aaaacgatag
     7381 cggtagggcg attgaaaaac tcggcaatct ctttctctaa cgccaggtgc ggcgcataac
     7441 tgccgtttgc catacgagaa ccggtggtgc cggtgccttg ggccgctaac gctgcctgac
     7501 cttcgctgat ggcatcatga ttaaaggtta aaccgagata attattggtc ccggccagaa
     7561 tcactttttt attgccgatc cggccttcgg tggcggaata cacttcatca atacaggtgc
     7621 caaacggtgt taacccgttg tcactaaatt gcagtcgctc gccagcaagg cgtgcatatt
     7681 tatcgtatag ccccattatt tattctccag ccatggaagt agagtttcca ttaattgcgc
     7741 aggggttttg acatccagca gaatattaat aggaatggaa atatcgaatc tgtcttccag
     7801 catcattaag agatccatca ctttgatcga ctccaggcca agatcgttga cgagatcgct
     7861 gtcgggctta atttccacac cgttttcgac caaatcctgt aaacaggaaa ggatgtaatc
     7921 cattactatt tcacgattta ccatagaaac gttaacacac ctttaaacaa gacaatcaaa
     7981 atagcccgtt gcgtaatgcg tgttccaggc taatcccggg aaaccaatta atatcttcag
     8041 aaatacggtt attacttgcc gaccagtcgg cgtgggttaa ttcacgaatt ttcgagcggg
     8101 tcagcatagg ttctttacct gccaggcgac tcaacgcggt actgatatcc gcaaggcagg
     8161 tgagtaccgg cagaggaata ccaaccattc gcacggaacc gcaacggacg ttggcggcaa
     8221 gttgctgtat gcgttgccag tcatagccgc cagcgacgct atcgcataat tcataggttt
     8281 gcgtctgtac agtttcggcg cttaaccact gacccactgc ttgcgcaaaa tcggtgacgt
     8341 gcaggaaaga gagctgtgta tctggtgcac caagtcgtgg cagcaggccg cgcagcatcc
     8401 agtcaaacag cggttttaac tctttatcgc cgggaccata cacggctgtc gggcgaaaaa
     8461 cgcccagcgt aatttcgtca gccattgcag tcagccgttg ttcggcgatg tgtttggaat
     8521 ttgcgtacca ggagagctcg ggatggcgcg ccgccagcga agagataaac agaaaacgtt
     8581 ggcaaaagca gctctctttt gctgcttgca tcaggcgcag gctgccgtca acgttacagc
     8641 gggtgaaaat ctcttctttg tgcccgcgca cttgcccggc gcaatggacc actgcgctgg
     8701 ctccggcaac cagcttgcta agtgaatgag tatcttccag cgaaccgcgc acccaggtga
     8761 gattatcatt gacgtgagcg cgggcagtac gcgtcaatgc gcgaacgtga aagccgcggg
     8821 cgagcaggtt atcgataata tatttaccga taaacccggt agcgcccgtc accgcgacag
     8881 tttgattcat gccagggatt cctgcacatt aagactggca gcataagcct tctgataacg
     8941 ttttttcgct tccgcacggg caggcttgcc ggaggacgtt cggggaatac tgtggggcgg
     9001 caacagatcg atagccgcgg tcacgccaaa ttcgctttgg atccgtgccg ccagcgcgtg
     9061 gataagctgc ccgcgacgtt cttcgtcgct gatccgacac tggatctgca aaatgatttt
     9121 ttcctgggcg gtaacaaaag caatcgcatc gccagaatga atttccggtt cttgttccgc
     9181 aatatattca atatcctgcg gccagatatt acggccacga ataataatca gatctttaat
     9241 gcgtccggtg acatacagat aaccgtccag cagataaccg aggtcgccgg tgtctaacca
     9301 gcccgtcgcg gcaatctcgt cttgcgaaac ctggtcgcca aagtaaccgc tcatcaggct
     9361 ggggccggag atgcaaatat ggcctaccac acgttccgcg accggcatac ctgcttcatt
     9421 gcggatttca atgccatgtt ccggcaacgc tttgccgcag ttgacgaaag tcgatacggc
     9481 gcgtgtctct gcacccggcg cgacggcttt accctgatat tcgaggatgt cgcgatccac
     9541 ttcgttaacc acaaccccgg aggcttcatc agagaagctg acagccagcg cattttctgc
     9601 cagtccgtag cacggcatga aagttttatt gtcaaagtta acctgacgga aacattcagc
     9661 gaattgatgg agttgttctg cggagatggg ttctgcacca ataccagcga cgcgccagca
     9721 ggaaagatcc agttcagcga gatctttttc attcacgcgg cgctggcaca attcatagcc
     9781 aaacggcggc gcaacggaaa cggtgccgcg atttttactg atcaatttaa gccattgcag
     9841 aggacgcatg gcaaaatcct gagtgcgcaa ataatctact gaaagctgcg tggcgacggg
     9901 gggcaggaga aagccgacca gtcccatatc atggtagaaa ggcagccagg agacgcagcg
     9961 gtcgccaggg cgtaatttaa tgccgtcgtg gcttatagca cgtagattag ccatcacttc
    10021 gcgatgggtg ataatgacgc cacggggaaa acgggtgctg ccggaggtgt actggaggta
    10081 ggcgatatcg ttcggaactg gacgctggag cgcaacatcg gcttccggta atgccttaaa
    10141 ccaggcgtgg cttaaaacat gtaattcggg gttgttatgc gtcgcggcat tgaccagtgg
    10201 caaccactca tcaccagtga taatggctgc gggctggcaa cttgccagta aaccctggag
    10261 tttggcgctc caggaatccc gctgaccgac gcccattgga atcgccaacg ggacggcgac
    10321 taagccagca tactgacagg caaaaaaagc ctctacaaac ccgctactcg tttcggcaat
    10381 cagcgccacg cgttcgcctt ttttcaggtt cagcgataac aaccgcttcg caccagcttc
    10441 ggcacgcgtt tttaatgtct gatattccag ttgatcttca agttggcaac gtctgtcata
    10501 aaaattcatt ccggcgctac tccgagcggc gtagtccaaa gcatcaacca gcgttggaaa
    10561 atcggcatag cgcataggta gggaatgcgt aaagatttta ttagacatat acaccacgaa
    10621 aaaattggat atcaaaaata aaatgacaat aa
//
LOCUS       DAZKHF020000155        10553 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2201S31241
            SAMN43267800-rid25106413.denovo.156, whole genome shotgun sequence.
ACCESSION   DAZKHF020000155 DAZKHF020000000
VERSION     DAZKHF020000155.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267800
            Sequence Read Archive: SRR30307263
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 10553)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10553)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 10553)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350581.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350581.2
            Genome Coverage       :: 151x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:21:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,652
            CDSs (total)                      :: 5,557
            Genes (coding)                    :: 5,320
            CDSs (with protein)               :: 5,320
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 237
            CDSs (without protein)            :: 237
            Pseudo Genes (ambiguous residues) :: 0 of 237
            Pseudo Genes (frameshifted)       :: 102 of 237
            Pseudo Genes (incomplete)         :: 130 of 237
            Pseudo Genes (internal stop)      :: 52 of 237
            Pseudo Genes (multiple problems)  :: 41 of 237
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10553
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267800-rid25106413.denovo.156"
                     /serotype="O 26:H11"
                     /isolate="2201S31241"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-01"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            complement(100..2091)
                     /gene="tkt"
                     /locus_tag="ACHOSC_RS15995"
                     /old_locus_tag="pgaptmp_003199"
     CDS             complement(100..2091)
                     /gene="tkt"
                     /locus_tag="ACHOSC_RS15995"
                     /old_locus_tag="pgaptmp_003199"
                     /EC_number="2.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004802 - transketolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HGU4549095.1"
                     /translation="MSSRKELANAIRALSMDAVQKAKSGHPGAPMGMADIAEVLWRDF
                     LKHNPQNPSWADRDRFVLSNGHGSMLIYSLLHLTGYDLPMEELKNFRQLHSKTPGHPE
                     VGYTAGVETTTGPLGQGIANAVGMAIAEKTLAAQFNRPGHDIVDHYTYAFMGDGCMME
                     GISHEVCSLAGTLKLGKLIAFYDDNGISIDGHVEGWFTDDTAMRFEAYGWHVIRDIDG
                     HDAASIKRAVEEARAVTDKPSLLMCKTIIGFGSPNKAGTHDSHGAPLGDAEIALTREQ
                     LGWKYAPFEIPSEIYAQWDAKEAGQAKESAWNEKFAAYAKAYPQEAAEFTRRMKGEMP
                     SDFDAKAKEFIAKLQANPAKIASRKASQNAIEAFGPLLPEFLGGSADLAPSNLTLWSG
                     SKAINEDAAGNYIHYGVREFGMTAIANGISLHGGFLPYTSTFLMFVEYARNAVRMAAL
                     MKQRQVMVYTHDSIGLGEDGPTHQPVEQVASLRVTPNMSTWRPCDQVESAVAWKYGVE
                     RQDGPTALILSRQNLAQQERTEEQLANIARGGYVLKDCAGQPELIFIATGSEVELAVA
                     AYEKLTAEGVKARVVSMPSTDAFDKQDAAYRESVLPKAVTARVAVEAGIADYWYKYVG
                     LNGAIVGMTTFGESAPAELLFEEFGFTVDNVVAKAKELL"
     gene            2369..3127
                     /gene="loiP"
                     /locus_tag="ACHOSC_RS16000"
                     /old_locus_tag="pgaptmp_003200"
                     /gene_synonym="yggG"
     CDS             2369..3127
                     /gene="loiP"
                     /locus_tag="ACHOSC_RS16000"
                     /old_locus_tag="pgaptmp_003200"
                     /gene_synonym="yggG"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311838.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metalloprotease LoiP"
                     /protein_id="HGU4549096.1"
                     /translation="MKIRALLVAMSVATVLTGCQNMDSNGLLSSGAEAFQAYSLSDAQ
                     VKTLSDQACQEMDSKATIAPANSEYAKRLTTIANALGNNINGQPVNYKVYMAKDVNAF
                     AMANGCIRVYSGLMDMMTDNEVEAVIGHEMGHVALGHVKKGMQVALGTNAVRVAAASA
                     GGIVGSLSQSQLGDLGEKLVNSQFSQRQEAEADDYSYDLLRQRGISPAGLATSFEKLA
                     KLEEGRQSSMFDDHPASAERAQHIRDRMSADGVK"
     gene            complement(3183..3926)
                     /locus_tag="ACHOSC_RS16005"
                     /old_locus_tag="pgaptmp_003201"
     CDS             complement(3183..3926)
                     /locus_tag="ACHOSC_RS16005"
                     /old_locus_tag="pgaptmp_003201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_462666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydro-3-deoxy-phosphogluconate aldolase"
                     /protein_id="HGU4549097.1"
                     /translation="MQKINFYRNRVAINVLAKDIANAREIFDAAEGHAVIGVLSAQFS
                     SVDEGIQEVKRWMAEIPSISVGLGAGDPAQYYKAAMIAAQIHPAHVNQTFTGCGFAAG
                     ALAATGGEQTHVNALVSPTGTPGEVLISTGVSSSQGTPARVSCDTAVRMMLDMGAHAA
                     KFFPMGGERSLPELYALATTAARNGMTLIEPTGGIDLDNFSVILKTCLEAGVPRIMPH
                     VYSSIIDPDTGYTRPDDVAVLLNKVKSLL"
     gene            complement(3913..5022)
                     /gene="dgaE"
                     /locus_tag="ACHOSC_RS16010"
                     /old_locus_tag="pgaptmp_003202"
     CDS             complement(3913..5022)
                     /gene="dgaE"
                     /locus_tag="ACHOSC_RS16010"
                     /old_locus_tag="pgaptmp_003202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_462667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-glucosaminate-6-phosphate ammonia lyase"
                     /protein_id="HGU4549098.1"
                     /translation="MTQNIYQQLGLKQVINACGKMTILGVSSVAPEVMQATARAASSF
                     VEIDQLVDKTGELVSRFTGAEDSYVTSCASAGIAIAVAAAITRGDKARVALMPDSSGM
                     ANEVVMLRGHNVDYGASILSAIRLGGGRVVEVGSSNLAALWQLESAITDATAALLYVK
                     SHHCVQKGMLNIEDFAHVAQMYNLPLIVDAAAEEDLRAWVVSGADMVVYSGAKAFNAP
                     TSGVITGKKIWIAACKAQHHGIARAMKIGKENMVGLVYALENYHQGQAVITAEQLQPV
                     VEAISAIHGLTADIEQDEAGRAIWRIRIRVNAQELGVDARVVEAQLRGGDIAIYARRY
                     NLHQGVFSLDPRTVAEGEMALIVARLKEIADHAKD"
     gene            complement(5026..5886)
                     /locus_tag="ACHOSC_RS16015"
                     /old_locus_tag="pgaptmp_003203"
     CDS             complement(5026..5886)
                     /locus_tag="ACHOSC_RS16015"
                     /old_locus_tag="pgaptmp_003203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_462668.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HGU4549099.1"
                     /translation="MMSDDVMQHELVERARESGALTKADITKAWFIYWLGAEVSSSYE
                     RLQSLIFCASMTPIIKKLYPQKEEQVEALKRHLNFFNSEQTFGAVIQGISIAMEEQKT
                     RGEAINDASITGIKTGLMGPLAGMGDSIIWAAVMPLLIAIFIPFAANGSAMGGILPLI
                     LYPAITLAISYGMVHKGYTLGRDSIISLLQGGRIKELIYGANVLGLIMMGALSASFVK
                     ITSPLKISALKGSEVVVQQILDSIAPGLLPLAAVFAIYFYLVKKGPRYTTILLSIVAL
                     SVVCSLLGVL"
     gene            complement(5883..6632)
                     /locus_tag="ACHOSC_RS16020"
                     /old_locus_tag="pgaptmp_003204"
     CDS             complement(5883..6632)
                     /locus_tag="ACHOSC_RS16020"
                     /old_locus_tag="pgaptmp_003204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_462669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS
                     mannose/fructose/sorbose/N-acetylgalactosamine transporter
                     subunit IIC"
                     /protein_id="HGU4549100.1"
                     /translation="MDTLLFASLMGLYYWFARLRLGYTFSAMLLQPVVVAVFVGLLLG
                     NMPAAMIIGAGMQLVYLGVTSTPGGNVPSDPALAACIAIPIAVKANMDPNLAIALAIP
                     FGVIGVFLDQLRRTLNAAWVHMADKHAETANMSAIMRCAFLYPALLGLVLRFPVVFAA
                     NYFGQNVVESFLKLMPHWLTHSFEIMGGILPALGFAITIMVIGKKSLLPWFIGGFFAV
                     LYLKVDIMAMAIFGTCVAFLIKGLAKNEGAA"
     gene            complement(6658..7143)
                     /locus_tag="ACHOSC_RS16025"
                     /old_locus_tag="pgaptmp_003205"
     CDS             complement(6658..7143)
                     /locus_tag="ACHOSC_RS16025"
                     /old_locus_tag="pgaptmp_003205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_462670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system
                     mannose/fructose/N-acetylgalactosamine-transporter subunit
                     IIB"
                     /protein_id="HGU4549101.1"
                     /translation="MANIVLCRIDSRLIHGQVVTKWVGQSQANRIAVVSDELDADPFM
                     KNIYLMAAPPNIKVDCFSNQSFAAAWKENQLGDGNVLVLFPSLAAIQEAVQQGFDVAD
                     IQVGGLGGGPNRKAVFQNITLDEKDVGILNDLKSRGIKAVFQTIPEDKPQSLDDILKK
                     F"
     gene            complement(7154..7582)
                     /locus_tag="ACHOSC_RS16030"
                     /old_locus_tag="pgaptmp_003206"
     CDS             complement(7154..7582)
                     /locus_tag="ACHOSC_RS16030"
                     /old_locus_tag="pgaptmp_003206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000214201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HGU4549102.1"
                     /translation="MTTTVPIHPLPEILLLTHGGWGLQLCNSLRMVMGEIEGVTEIAL
                     MPVDTLGEFYQRVEAVVKTMPEGSLIVTDFIGGTTSNVAARLSADYPVAVISGLNASL
                     LLEALDKREHGPLTACVADLVEAGRNSCLDVVSHIRQLQQ"
     gene            complement(7701..10499)
                     /gene="dagR"
                     /locus_tag="ACHOSC_RS16035"
                     /old_locus_tag="pgaptmp_003207"
     CDS             complement(7701..10499)
                     /gene="dagR"
                     /locus_tag="ACHOSC_RS16035"
                     /old_locus_tag="pgaptmp_003207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_462672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator DagR"
                     /protein_id="HGU4549103.1"
                     /translation="MRRIDVIHKELERLTYGLELSDLAKEKAFTAEAIGFNLGLARNS
                     VSKDLNQLWNEGLVVKSQGRPVFFLHRHALELLINRKLDDCECIVHSVASLLPKKEKY
                     TDDDPFSGLIGYDRSLRDAVEKGRAAVLYPHGLHVLLTGASGVGKTFFAELMHCFACK
                     HTIGSPPPLVYFNCAEYAHNPELLSSHLFGHHKGAFTGASENKTGLVEQADGGYLLLD
                     EVHRLPYEGQEKLFSILDKGEYRPLGSSGPTYSISVRLICATTESVNSALLRTFQRRI
                     QVCIDLPGIRQRSVEEQLELIVSFFQRESRKIERTISLDKTLLHWLLSKPLEGNIGQL
                     KSDIQFLCAQAWASGMTERNDTLELDKRLAEMSFNTTPEQRLLVDALFGGKDRLNVDA
                     RTLPALKNSLATSAEIEESDLFYSFLTREYVNLRNSNVPPAETLAILKNKLSSIFEYG
                     LYSRHSAAHPPRYGDQIEERVTLLIGYVEQVLGFTLPENLANPLRKHFLALIGYVRRG
                     LIPQLYSSSLILDRCKDEYDNATLLCRKINELLHIQCPATEVVWLCLFLKECRHYRQR
                     IDTSPDCGVILIAHGATTATSQAQYVNRVLERDLFYAIDMPFEQSVHDTLETLTRMIQ
                     DRCWQRLILMVDIGSLVHFGSTISKLFQIDVLLLPNITLTSLLEVGLDLSYETGDLSQ
                     LAVLMQSKNIPCRLCTPQQESGGKVLVISCITGMGTAEKIKKVLEESFGELMSQDTRM
                     VILDYNEVRSLERIQQALNASERLAGIVGTFQPGLPDIPFISLEELFSEQGPELVLSL
                     LTPDLSSSERRLEMERSAMRFISALTMESIINHISVLNPQRILKEIEDVLNYLTNTLS
                     LKPSRQVTLRFLIHCCCMVERIVINRKPLQMALENRLDLDARAFSVIKSSFLPIEEAY
                     AIRLSDAEYFYIYELLYS"
BASE COUNT         2492 a         2999 c         2696 g         2366 t
ORIGIN      
        1 tgtgatctac aacacgcttt atctattgct tgtccgcaaa cggacatatc aaggtaataa
       61 aaaaggtcgc cgaagcgacc tttttacccg aaatgctaat tacagcagtt cttttgcttt
      121 cgcaacaacg ttatcaacag tgaagccgaa ctcttcaaac agcagctctg ccggagcaga
      181 ttcaccgaag gtggtcatac cgacgatagc accgttcagg ccaacatact tgtaccagta
      241 gtcagcaata cccgcttcta cagcaacgcg tgcagtaacc gctttcggca gtacggattc
      301 acggtaagca gcatcctgct tgtcaaatgc gtcggtagac ggcatggaca ccacgcgcgc
      361 tttcacgcct tcggcagtca gtttttcgta ggcagcaaca gccagttcaa cttctgaacc
      421 ggtagcgatg aaaatcagtt ccggctgacc ggcgcagtct ttcagcacat aaccaccgcg
      481 cgcgatgttt gccagttgct cttcagttcg ttcctgctgc gccaggttct gacgggagag
      541 gatcagtgcg gtcgggccgt cctgacgctc aacaccgtat ttccacgcga ccgcggattc
      601 aacctggtca cacggacgcc atgtagacat gttcggggtt acgcgcagag aagcgacctg
      661 ctctaccggc tggtgagtcg ggccatcttc gcccagaccg atggagtcgt gggtgtaaac
      721 catcacctga cgctgtttca tcagcgcagc catacgtacg gcgttacgtg cgtattccac
      781 gaacatcagg aaggtggagg tgtacggcag gaaaccaccg tgcagggaga taccgttagc
      841 aatcgcggtc ataccgaact cgcgaacacc gtagtggatg tagttacccg cagcatcttc
      901 gttgattgct ttagaaccag accacagggt caggttagac ggtgccaggt cagcagaacc
      961 gccgaggaat tccggcaaca gcggaccgaa agcttcgata gcattctgag acgctttacg
     1021 gctggcgatt ttcgccgggt tagcctgcag tttagcaatg aactcttttg ctttcgcgtc
     1081 gaagtcagac ggcatttcgc ctttcatacg gcgggtaaat tcagcggctt cctgcggata
     1141 agctttcgcg taagcagcga atttctcgtt ccatgcggat tctttcgcct ggcctgcttc
     1201 tttcgcatcc cactgagcat agatttcaga cgggatttcg aacggtgcat atttccagcc
     1261 cagctgttcg cgggtcaggg caatttcagc gtcgcccagc ggcgcaccgt gggagtcgtg
     1321 agtaccggct ttgttcgggg aaccgaaacc gatgatggtt ttgcacatca gcagggacgg
     1381 tttgtcagtc actgcgcgcg cttcttctac tgcgcgtttg atggatgccg cgtcatgacc
     1441 gtcgatgtcg cgaataacgt gccagccgta agcttcgaaa cgcattgcgg tgtcgtcggt
     1501 gaaccagcct tcaacgtgac catcaataga gataccgttg tcatcgtaga acgcaatcag
     1561 tttacccagc ttcagcgtac ccgccagaga gcaaacttcg tgggagatgc cttccatcat
     1621 gcagccgtcg cccatgaagg cgtaggtgta gtggtcgaca atgtcgtggc ccggacggtt
     1681 aaactgcgcc gccagcgttt tttctgcaat cgccatacct actgcgttgg caataccctg
     1741 ccccagcgga ccggtggtgg tttccacacc agcggtgtaa cccacttccg ggtggcccgg
     1801 agttttagag tgcagctgac ggaagttttt cagttcttcc atcggcagat cgtaaccggt
     1861 gaggtgcagc aggctgtaga tcagcatgga gccgtggccg ttggacagca cgaagcggtc
     1921 acggtcagcc caggacggat tctgcgggtt gtgtttcagg aaatcacgcc acaggacttc
     1981 ggcaatgtca gccataccca taggggcacc cgggtgaccg gatttggctt tctgtactgc
     2041 gtccatgctc agcgcacgaa tagcattggc aagctcttta cgtgaggaca ttttgactcc
     2101 agatcggatg atgaagggca cgcccttaac gacttgacga cagcgcgttt tgggctacgc
     2161 cggaaaattt gccaacaatt taccgcaagc cgcgcgtcat gtacatggaa catccttttg
     2221 ccgcttcaga aatctctgga tcatgctcgc atgttgcgca atctactcgc ccgtccgctg
     2281 cgcttttcct tatactgaga ctgagcgtcg attcacctgc aaacggcgca tttttagaat
     2341 aatcctgacc ttgtgcggaa gagaaaacat gaaaattcgc gccttattgg tagcaatgag
     2401 cgtggcaacg gtactgactg gttgccagaa tatggactcc aacggactgc tctcatcagg
     2461 agcggaagct tttcaggctt acagtttgag tgatgcgcag gtgaaaaccc tgagcgatca
     2521 ggcatgtcag gagatggaca gcaaggcgac gattgcgcca gccaatagcg aatacgctaa
     2581 acgtctgaca actattgcca atgcgctagg caacaatatc aacggtcagc cggtgaatta
     2641 caaagtgtat atggcgaagg atgtgaacgc ctttgcaatg gctaacggct gtatccgcgt
     2701 ctatagcggg ctgatggata tgatgacgga taacgaagtc gaagcggtga tcggtcacga
     2761 aatggggcac gtggcgttag gccatgtgaa aaaaggaatg caggtggcac ttggtacaaa
     2821 tgccgtgcga gtagctgcgg cctctgcggg cgggattgtc ggaagtttat ctcaatcaca
     2881 acttggtgat ctgggcgaga aattagtcaa ttcgcaattc tcccagcgcc aggaagcaga
     2941 agccgatgat tattcttacg atcttctgcg ccaacgcggc atcagcccgg caggtcttgc
     3001 caccagcttt gaaaaactgg caaaactgga agaaggtcgc caaagctcaa tgtttgacga
     3061 ccatcctgca tccgccgaac gcgcccagca tattcgcgat cgtatgagcg cggatggggt
     3121 taagtaagtc agacataccg atctacaaca taattgtagg tcggtaacgc ctgaatcccc
     3181 gatcagagta gtgatttcac tttatttagc aaaacggcta catcatccgg tcgggtatat
     3241 cccgtatcag ggtctataat cgagctgtaa acatgtggca taatgcgcgg cacccccgcc
     3301 tccagacaag ttttcagaat cacactgaag ttatccaaat caatcccgcc agtcggctca
     3361 atcagcgtca taccattgcg cgccgccgtg gtcgccagcg cgtatagctc cggaagcgac
     3421 ctctcgccgc ccatcggaaa gaacttcgcc gcgtgtgcgc ccatatctag catcattcgc
     3481 accgccgtat cgcaggagac gcgggcaggc gtaccctggc tgctgctaac gccggtggaa
     3541 atcagcactt cgcccggcgt ccccgtcggg ctaaccagcg cgttgacatg ggtctgctca
     3601 ccgcccgtag ccgcaagcgc gcctgccgcg aaaccacaac cggtaaaggt ctggttaacg
     3661 tgtgctggat gaatctgcgc cgcaatcatc gcagctttgt aatactgcgc cgggtcaccc
     3721 gcccccagac ctactgaaat ggacggtatt tccgccatcc aacgtttaac ttcctggatc
     3781 ccctcatcca ccgaggagaa ctgcgcggaa agcacgccaa tcactgcgtg gccttcggcg
     3841 gcgtcaaaga tttcacgggc attggcgata tctttcgcca gcacattaat tgccacgcga
     3901 ttacgataga aattaatctt ttgcatgatc tgcaatctcc ttcagtcgcg ccacgataag
     3961 tgccatttcc ccttccgcca ccgtacgcgg atcaaggcta aaaacgccct ggtggaggtt
     4021 ataccgccgt gcataaatcg cgatatcgcc accacgcagt tgcgcttcga cgacgcgagc
     4081 gtcaactccc aattcctgcg catttactcg aatacgaata cgccagattg cacgcccggc
     4141 ttcatcctgc tcaatatcag ccgttaaccc gtgaatcgca gaaatcgctt caaccactgg
     4201 ctggagctgt tccgcggtga tgactgcctg cccctggtga taattctcca gcgcgtaaac
     4261 cagcccaacc atgttctcct tgccgatttt catcgccctg gcaatgccgt gatgctgcgc
     4321 tttgcaggct gcaatccaga tttttttacc agtgataacc ccggaggtcg gcgcgttaaa
     4381 ggcttttgcg ccgctgtaga ccaccatatc cgctccgctc accacccagg cacgtaaatc
     4441 ctcttcggcg gcggcatcga caatcagcgg caggttgtac atctgcgcga catgtgcgaa
     4501 gtcctcgatg ttcaacattc ccttctgcac gcagtgatgc gacttcacat acagcagcgc
     4561 ggcggtcgcg tcggtaatgg cactctccag ctgccagaga gccgccaggt tacttgaacc
     4621 cacctccaca acgcgcccgc cacctaagcg gatcgcgctg aggatcgatg caccgtaatc
     4681 gacgttatgg ccgcgcagca tcaccacttc attggccatg ccggagctat ccggcatgag
     4741 cgccacccgg gccttgtcgc cgcgtgtaat ggcggcggcg acagcgatgg cgatccctgc
     4801 tgaggcacag gaggtaacgt agctatcctc cgccccggta aagcgagaga ccagctcgcc
     4861 ggttttgtcc accagctggt caatctccac gaaagaggat gccgcgcgcg cggtggcctg
     4921 catcacctcc ggcgcgacgc tggagacgcc gagaatcgtc atcttgccgc aggcgttaat
     4981 cacctgtttc agtcccagtt gttgatagat attctgcgtc atggcttaca gcactcccag
     5041 cagcgagcag accacgctca acgccacaat cgacagcaga atcgtggtgt aacgcggacc
     5101 ctttttcacc aaatagaagt agatggcgaa cactgccgcc agcggcagca ggccgggcgc
     5161 gatggagtcc agaatctgct gtaccaccac ttcagagcct ttcagtgcgc taatttttaa
     5221 aggtgaggtt atcttgacaa agctcgcgga tagcgcgccc atcatgataa gccccagtac
     5281 gttagcgccg tagataagct ccttgatgcg ccctccctgc agcagactga taatcgaatc
     5341 gcgccccagc gtatagccct tgtgcaccat gccgtagctg attgccagcg taatcgccgg
     5401 gtagagaatc agcggcaaaa taccacccat cgcgctgcca ttggcggcga acgggataaa
     5461 aatagcaatc aacagcggca tcaccgccgc ccagatgatg gagtcgccca tcccggccag
     5521 cggccccatc aggccggttt taatcccggt gatagaggca tcgttaatcg cctcgccgcg
     5581 ggtcttttgc tcttccatgg cgatggagat cccctggatc accgcgccaa acgtctgctc
     5641 tgagttgaag aagttcagat ggcgttttaa cgcctccacc tgctcctcct tttgcggata
     5701 gagctttttg ataatcggcg tcatcgaggc gcagaaaatc aggctctgta aacgctcata
     5761 ggagctggaa acttccgcgc ccagccagta gataaaccac gctttggtga tatccgcttt
     5821 ggtcagcgcc ccggactccc gcgcgcgctc aaccagttca tgctgcatta cgtcgtcgct
     5881 cattatgccg ctccttcatt tttcgccagg cctttaatca ggaaggccac gcaggtgccg
     5941 aaaatcgcca tcgccatgat gtccaccttg aggtacagca ccgcgaagaa tccgccgata
     6001 aaccacggca gcaggctctt tttaccaatc accataatgg tgatggcgaa gcccagcgcc
     6061 gggagaatcc cgcccatgat ttcaaacgag tgggtcagcc agtgcggcat cagcttgagg
     6121 aaactttcca ctacgttctg gccgaagtag ttggcggcga acaccactgg gaaacgtagt
     6181 accagcccca gcagcgccgg gtagagaaag gcgcagcgca taatggctga catattggcc
     6241 gtttccgcgt gtttatcggc catatgcacc caggccgcat ttagcgtgcg acgcaactgg
     6301 tcgaggaata cgccgataac gccaaatgga atcgccagtg caatcgccag gttcgggtcc
     6361 atattggcct taacggcgat aggaatggcg atacaggccg ccagcgccgg gtctgacggc
     6421 acattgccgc ctggcgttga ggtcacaccc agatagacca actgcatccc cgcgccgata
     6481 atcatcgccg caggcatatt gccgagcagc aggccgacaa agaccgccac caccactggc
     6541 tgtaacagca tggcggagaa ggtatagcca aggcgcaacc gggcaaacca gtaatacagc
     6601 cccatcaggc ttgcaaaaag taaggtatcc ataatttgtt atcccatgtt cagagggtca
     6661 gaattttttc aggatatcgt ccagcgactg cggtttatct tccgggatgg tctggaaaac
     6721 agccttaatg ccgcgacttt tcaaatcgtt aagaatgcca acgtcttttt catccagcgt
     6781 tatgttctgg aacaccgctt tacggtttgg cccgccgcct aaaccgccaa cctgaatatc
     6841 agccacgtca aagccctgct gcaccgcttc ctgaatcgcc gccagcgacg ggaacagcac
     6901 cagcacgttg ccgtcgccca gctggttctc tttccacgcg gccgcgaagc tctggttgct
     6961 aaagcagtcc actttgatat tcggcggcgc ggccatcagg tagatatttt tcataaacgg
     7021 atcggcgtcc agctcgtcgc tgaccaccgc aatacggttg gcctgagact gccctaccca
     7081 cttcgtcacc acctgtccgt ggataagacg gctgtcaatg cgacataaca cgatgtttgc
     7141 catacgattt tccttactgt tgtaattgac gaatatggga gacgacgtcc aggcaactgt
     7201 tgcgcccagc ttccaccaga tcggccacgc aggcggtcag tgggccatgt tcgcgcttgt
     7261 ccagcgcctc cagcagcagc gaggcattaa gcccggagat caccgccacc ggataatcgg
     7321 cgctcagccg cgccgccacg ttagaggtgg tgccgccgat aaaatcggtc acgataagcg
     7381 aaccttccgg catggttttc accacggctt cgacgcgctg gtaaaactcg cccagcgtat
     7441 cgacgggcat cagcgcgatt tccgtcacgc cttcgatttc gcccatcacc atgcgcagac
     7501 tgttgcagag ctgcagcccc cagccgccgt gagttagcag cagaatttca ggtaacggat
     7561 gaataggtac agttgtggtc aaaatagcct cctttgctcc agtgtttaca tttcatcagg
     7621 agcaaaggac gtgccagaat ttgtgtcacg cacagaggaa atgagattgg atggggcgtt
     7681 aagccccgta aaccagggga tcagctgtag agcagttcgt agatataaaa atattctgcg
     7741 tccgataaac ggatagcgta agcctcttca atcggcaaaa aggaggattt gatgacgcta
     7801 aaggcacgag cgtccagatc aagccggttt tctagcgcca tctgtaacgg tttgcggtta
     7861 atcacgatac gctcgaccat gcagcagcag tggatcagaa aacgtagtgt cacctggcgg
     7921 ctcggtttca gggagagcgt gttggttaag tagttcagaa catcctcgat ctctttcaga
     7981 attcgctgcg ggttgagcac cgaaatatgg ttgatgatgc tctccatggt cagcgcgctg
     8041 ataaagcgca tggcgctgcg ctccatctcc agtcggcgct cgctgcttga gagatccggc
     8101 gtcagcaggc tgagcaccag ctccggccct tgctcggaaa acagttcctc cagcgagata
     8161 aacggtatat ccggcagtcc cggctggaag gtgccgacga tccctgccag ccgttcactg
     8221 gcattaagcg cctgctgaat acgctccagg ctgcgcacct cgttatagtc gagaatcacc
     8281 atacgcgtgt cctgcgacat cagctcgcca aagctctcct ccagcacctt tttgatcttt
     8341 tccgccgtcc ccattccggt tatacatgag ataaccagca ctttgccacc gctctcttgc
     8401 tgcggcgtgc agagccgaca agggatattt ttactctgca taagcacagc caattgcgat
     8461 agatcgccag tttcgtagct taaatccagc ccaacctcca gcaggctggt cagagtgata
     8521 tttggcaaca acagaacgtc aatctggaac agcttgctga tggtgctgcc aaaatggacc
     8581 agcgatccga tatccaccat cagtatcagc cgctgccagc accggtcctg aatcatccgg
     8641 gtcagggttt ccagggtgtc atgcaccgac tgctcaaatg gcatatcaat tgcgtagaac
     8701 agatcgcgct ccagcacccg gttcacatat tgcgcctggc tcgttgcggt ggtcgcaccg
     8761 tgagcgatta gaatcacgcc gcaatccggg ctggtgtcga tgcgctggcg gtagtggcga
     8821 cactctttca ggaacaggca cagccagacc acctccgtcg ccgggcactg aatatgcaac
     8881 aactcattga ttttgcggca cagaagcgtg gcattgtcat actcatcttt acagcgatcc
     8941 agaatcaggc tggatgaata gagttgaggt ataagccctc gccgcacata gccaatcagc
     9001 gcaagaaagt gtttacgtaa cgggttcgcc aggttttctg gcaatgtaaa tcctaatacc
     9061 tgctccacgt aaccgatcag cagcgtcaca cgctcctcaa tttgatcgcc atagcgcggt
     9121 ggatgcgccg cactatggcg gctataaaga ccatattcaa aaatcgaact gagtttgttt
     9181 ttcaggatag ccagcgtttc tgcgggcggc acgttactgt tgcgcagatt gacatattca
     9241 cgagtcagga aactgtaaaa caggtcgctc tcttctattt ccgcgctggt cgccaacgaa
     9301 ttttttaacg cgggcaacgt acgcgcatcc acgttcagac gatctttacc gccaaacaac
     9361 gcatcgacca gcagacgctg ctctggcgtg gtattaaacg acatctccgc cagtcgttta
     9421 tctaactcta gcgtatcatt acgctcggtc attcccgacg cccacgcctg cgcgcacaag
     9481 aactgaatat cacttttaag ctggccgata ttgccttcca ggggtttact taacaaccaa
     9541 tgcaagagcg ttttatcaag gcttatggta cgttcaattt tacgactttc acgctgaaag
     9601 aagcttacaa tcaattcgag ctgctcctcg accgaacgct ggcgaatacc cggtaagtca
     9661 atacatacct ggatacgccg ctggaaggta cgcaatagtg ccgagttgac cgactctgta
     9721 gtggcgcaaa taaggcgcac cgaaattgaa tatgttggcc cacttgaacc aagtgggcgg
     9781 tattcacctt tatcgagaat agaaaagagc ttctcttgtc cttcgtacgg gaggcgatgc
     9841 acttcatcca gaagtaaata acctccgtca gcttgctcca ccaatcccgt tttattctca
     9901 cttgccccgg taaatgcccc cttatggtga ccaaacagat gtgaggaaag caactccgga
     9961 ttatgagcat attcggcaca gttaaagtaa accaacggag gtggcgaacc tatggtgtgc
    10021 ttgcaagcga agcaatgcat taactcagca aagaatgttt tgccaacacc agatgcaccg
    10081 gttaacagca catgtagtcc gtggggataa agcaccgctg cgcggccttt ttctaccgca
    10141 tcacggagac tacggtcgta tccgataagt ccggaaaacg gatcatcatc tgtatatttt
    10201 tctttctttg gcaataaact agccactgaa tgaactatgc attcgcaatc atccagtttt
    10261 cgattaatga gcaattctag tgcgtgacga tgtagaaaaa aaacaggacg tccctgactt
    10321 tttaccacca ggccttcatt ccataattga ttaagatctt tactaaccga gttgcgtgcc
    10381 agcccaaggt taaaaccaat cgcctcagca gtaaatgctt tctccttcgc taaatcagac
    10441 agttccagcc catacgtcag ccgctccagc tccttgtgaa taacatcaat ccgtctcatt
    10501 tctttaccgc ttaaaaaaca atagttgttc agaacataca ctatttgtaa acg
//
LOCUS       DAZKHF020000156        10528 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2201S31241
            SAMN43267800-rid25106413.denovo.157, whole genome shotgun sequence.
ACCESSION   DAZKHF020000156 DAZKHF020000000
VERSION     DAZKHF020000156.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267800
            Sequence Read Archive: SRR30307263
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 10528)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10528)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 10528)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350581.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350581.2
            Genome Coverage       :: 151x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:21:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,652
            CDSs (total)                      :: 5,557
            Genes (coding)                    :: 5,320
            CDSs (with protein)               :: 5,320
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 237
            CDSs (without protein)            :: 237
            Pseudo Genes (ambiguous residues) :: 0 of 237
            Pseudo Genes (frameshifted)       :: 102 of 237
            Pseudo Genes (incomplete)         :: 130 of 237
            Pseudo Genes (internal stop)      :: 52 of 237
            Pseudo Genes (multiple problems)  :: 41 of 237
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10528
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267800-rid25106413.denovo.157"
                     /serotype="O 26:H11"
                     /isolate="2201S31241"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-01"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            complement(10..582)
                     /locus_tag="ACHOSC_RS16040"
                     /old_locus_tag="pgaptmp_003208"
     CDS             complement(10..582)
                     /locus_tag="ACHOSC_RS16040"
                     /old_locus_tag="pgaptmp_003208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000227281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4549104.1"
                     /translation="MVDASAQPHIVVTLEPNPWAAFYFDINIANTGNAPAYNVEVVFD
                     PPLVNAEHREKSEIPFSKVSVLKNGQSLTSNLCKYEQIKDQIYNINISWASKPKSNDR
                     ETNEYVYDMATFEGISYLGARSPLTQIAEQIKGIREDWKPIAQGAKKVKADVYTSSDR
                     NEERTYLQEQHDLAIKRRDEKREKRLESGE"
     gene            683..1853
                     /locus_tag="ACHOSC_RS16045"
                     /old_locus_tag="pgaptmp_003209"
                     /pseudo
     CDS             683..1853
                     /locus_tag="ACHOSC_RS16045"
                     /old_locus_tag="pgaptmp_003209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611482.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
     gene            1936..2193
                     /locus_tag="ACHOSC_RS16050"
                     /old_locus_tag="pgaptmp_003210"
     CDS             1936..2193
                     /locus_tag="ACHOSC_RS16050"
                     /old_locus_tag="pgaptmp_003210"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4549105.1"
                     /translation="MTLVNDTGFDPVFSGSIAESWRQQPCTPSYCCDWEAATMLRAFP
                     LAKKGEGRARLPSLYASFGKLGETPTHKDIIDNNRSINWPV"
     gene            complement(2751..3518)
                     /gene="fecE"
                     /locus_tag="ACHOSC_RS16055"
                     /old_locus_tag="pgaptmp_003211"
     CDS             complement(2751..3518)
                     /gene="fecE"
                     /locus_tag="ACHOSC_RS16055"
                     /old_locus_tag="pgaptmp_003211"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe(3+) dicitrate ABC transporter ATP-binding
                     protein FecE"
                     /protein_id="HGU4549106.1"
                     /translation="MTLRTENLTVSYGTDKVLNDVSLSLPTGKITALIGPNGCGKSTL
                     LNCFSRLLMPQSGTVFLGDNPINMLSSRQLARRLSLLPQHHLTPEGITVQELVSYGRN
                     PWLSLWGRLSAEDNARVNVAMNQTRINHLAVRRLTELSGGQRQRAFLAMVLAQNTPVV
                     LLDEPTTYLDINHQVDLMRLMGELRTQGKTVVAVLHDLNQASRYCDQLVVMANGHVMA
                     QGTPEEVMTPGLLRTVFSVEAEIHPEPVSGRPMCLMR"
     gene            complement(3519..4475)
                     /gene="fecD"
                     /locus_tag="ACHOSC_RS16060"
                     /old_locus_tag="pgaptmp_003212"
     CDS             complement(3519..4475)
                     /gene="fecD"
                     /locus_tag="ACHOSC_RS16060"
                     /old_locus_tag="pgaptmp_003212"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe(3+) dicitrate ABC transporter permease
                     subunit FecD"
                     /protein_id="HGU4549107.1"
                     /translation="MKIALVIFITLALAGCALLSLHMGVIPVPWRALLTDWQAGREHY
                     YVLMEYRLPRLLLALFVGAALAVAGVLIQGIVRNPLASPDILGVNHAASLASVGALLL
                     MPSLPVMVLPLLAFAGGMAGLILLKMLAKTHQPMKLALTGVALSACWASLTDYLMLSR
                     PQDVNNALLWLTGSLWGRDWSFVKIAIPLMILFLPLSLSFCRDLDLLALGDARATTLG
                     VSVPHTRFWALLLAVAMTSTGVAACGPISFIGLVVPHMMRSITGGRHRRLLPVSALTG
                     ALLLVVADLLARIIHPPLELPVGVLTAIIGAPWFVWLLVRMR"
     gene            complement(4472..5470)
                     /gene="fecC"
                     /locus_tag="ACHOSC_RS16065"
                     /old_locus_tag="pgaptmp_003213"
     CDS             complement(4472..5470)
                     /gene="fecC"
                     /locus_tag="ACHOSC_RS16065"
                     /old_locus_tag="pgaptmp_003213"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-dicitrate ABC transporter permease FecC"
                     /protein_id="HGU4549108.1"
                     /translation="MTAIKHPVLLWGLPVAALIIIFWLSLFCYSAIPVSGADATRALL
                     PGHTPTLPEALVQNLRLPRSLVAVLIGASLALAGTLLQTLTHNPMASPSLLGINSGAA
                     LAMALTSALSPTPIAGYSLSFIAACGGGVSWLLAMTAGGGFRHTHDRNKLILASIALS
                     AFCMGLTRIPLLLAEDHAYGIFYWLAGGVSHARWQDVWQLLPVVVTAVPVVLLLANQL
                     NLLNLSDSTAHTLGVNLTRLRLVINMLVLLLVGACVSVAGPVAFIGLLVPHLARFWAG
                     FDQRNVLPVSMLLGATLMLLADVLARALAFPGDLPAGAVLALIGSPCFVWLVRRRG"
     gene            complement(5467..6369)
                     /gene="fecB"
                     /locus_tag="ACHOSC_RS16070"
                     /old_locus_tag="pgaptmp_003214"
     CDS             complement(5467..6369)
                     /gene="fecB"
                     /locus_tag="ACHOSC_RS16070"
                     /old_locus_tag="pgaptmp_003214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418710.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe(3+) dicitrate ABC transporter
                     substrate-binding protein FecB"
                     /protein_id="HGU4549109.1"
                     /translation="MLAFIRFVFAGLLLVISHAFAATVQDEHGTFTLEKTPQRIVVLE
                     LSFADALAAVDVSPIGIADDNDAKRILPEVRAHLKPWQSVGTRAQPSLEAIAALKPDL
                     IIADSSRHAGVYIALQQIAPVLLLKSRNETYAENLQSAAIIGEMVGKKREMQARLEQH
                     KERMAQWASQLPKGTRVAFGTSREQQFNLHTQETWTGSVLASLGLNVPAAMAGASMPS
                     IGLEQLLAVNPAWLLVAHYREESIVKRWQQDPLWQMLTAAQKQQVASVDSNTWARMRG
                     IFAAERIAADTVKIFHHQPLTVVK"
     gene            complement(6414..8738)
                     /gene="fecA"
                     /locus_tag="ACHOSC_RS16075"
                     /old_locus_tag="pgaptmp_003215"
     CDS             complement(6414..8738)
                     /gene="fecA"
                     /locus_tag="ACHOSC_RS16075"
                     /old_locus_tag="pgaptmp_003215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent Fe(3+) dicitrate receptor FecA"
                     /protein_id="HGU4549110.1"
                     /translation="MTPLRVFRKTTPLVNAIRLSLLPLAGLSFSAFAAQVNIAPGSLD
                     KALNQYAAHSGFTLSVDASLTRGKQSNGLHGDYDVESGLQQLLDGSGLQVKPLGNNSW
                     TLEPAPAPKEDALTVVGDWLGDARENDVFEHAGARDVIRREDFAKTGATTMREVLNRI
                     PGVSAPENNGTGSHDLAMNFGIRGLSPRLASRSTVLMDGIPVPFAPYGQPQLSLAPVS
                     LGNMDAIDVVRGGGAVRYGPQSVGGVVNFVTRAIPQDFGIEAGVEGQLSPTSSQNNPK
                     ETHNLMVGGTADNGFGTALLYSGTRGSDWREHSATRIDDLMLKSKYAPDEVHTFNSLL
                     QYYDGEADMPGGLSRADYDADRWQSTRPYDRFWGRRKLASLGYQFQPDSQHKFNIQGF
                     YTQTLRSGYLEQGKRITLSPRNYWVRGIEPRYSQIFMIGPSAHEVGVGYRYLNESTHE
                     MRYYTATSSGQLPSGSSPYDRDTRSGTEAHAWYLDDKIDIGNWTITPGMRFEHIESYQ
                     NNAITGTHEEVSYNAPLPALNVLYHLTDSWNLYANTEGSFGTVQYSQIGKAVQSGNVE
                     PEKARTWELGTRYDDGALTAEMGLFLINFNNQYDSNQTNDTVTARGKTRHTGLETQAR
                     YDLGTLTPTLDNVSIYASYAYVNAEIREKGDTYGNLVPFSPKHKGTLGVDYKPGNWTF
                     NLNSDFQSSQFADNANTVKESADGSTGRIPGFMLWGARVAYDFGPQMADLNLAFGVKN
                     IFDQDYFIRSYDDNNKGIYAGQPRTLYMQGSLKF"
     gene            complement(8825..9778)
                     /gene="fecR"
                     /locus_tag="ACHOSC_RS16080"
                     /old_locus_tag="pgaptmp_003216"
     CDS             complement(8825..9778)
                     /gene="fecR"
                     /locus_tag="ACHOSC_RS16080"
                     /old_locus_tag="pgaptmp_003216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferric citrate uptake sigma factor regulator
                     FecR"
                     /protein_id="HGU4549111.1"
                     /translation="MNPLLTDSRRQALRSASHWYAVLSGERVSPQQEARWQQWYEQDQ
                     DNQWAWQQVENLRNQLGGVPGDVASRALHDTRLTRRHVMKGLLLLLGAGGGWQLWQSE
                     TGEGLRADYRTAKGTVSRQQLEDGSLLTLNTQSAADVRFDAHQRTVRLWYGEIAITTA
                     KDALQRPFRVLTRQGQLTALGTEFTVRQQDNFTQLDVQQHAVEVLLASAPAQKRIVNA
                     GESLQFSASEFGAVKPLDDESTSWTKDILSFSDKPLGEVIATLTRYRNGVLRCDPAVA
                     GLRLSGTFPLKNTDAILNVIAQTLPVKIQSITRYWINISPL"
     gene            complement(9775..10296)
                     /gene="fecI"
                     /locus_tag="ACHOSC_RS16085"
                     /old_locus_tag="pgaptmp_003217"
     CDS             complement(9775..10296)
                     /gene="fecI"
                     /locus_tag="ACHOSC_RS16085"
                     /old_locus_tag="pgaptmp_003217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor FecI"
                     /protein_id="HGU4549112.1"
                     /translation="MSDRATTTASLTFESLYGTHHGWLKSWLTRKLQSAFDADDIAQD
                     TFLRVMVSETLSTIRDPRSFLCTIAKRVMVDLFRRNALEKAYLEMLALMPEGGAPSPE
                     ERESQLETLQLLDSMLDGLNGKTREAFLLSQLDGLTYSEIAHKLGVSISSVKKYVAKA
                     VEHCLLFRLEYGL"
     gene            10433..10501
                     /gene="ykiE"
                     /locus_tag="ACHOSC_RS16090"
                     /old_locus_tag="pgaptmp_003218"
     CDS             10433..10501
                     /gene="ykiE"
                     /locus_tag="ACHOSC_RS16090"
                     /old_locus_tag="pgaptmp_003218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YkiE"
                     /protein_id="HGU4549113.1"
                     /translation="MGWSLCFWHVSVRMTHDVVCYY"
BASE COUNT         2385 a         2851 c         2917 g         2375 t
ORIGIN      
        1 cctttaaaat tattcaccag actctaatct tttttctctt ttctcatctc tcctttttat
       61 tgccaaatcg tgttgctctt gcagatacgt gcgttcttcg tttctatcgc ttgaagtata
      121 tacgtctgct tttacttttt tagctccttg tgcaataggt ttccaatcct ctcttatacc
      181 tttaatttgt tctgcaattt gcgtcaatgg gcttctcgct cctagataac ttattccttc
      241 aaatgtcgcc atgtcataca catattcatt tgtttctcta tcgtttgatt taggtttgct
      301 tgcccagctt atattaatat tataaatttg atctttgatt tgttcatact tacagagatt
      361 gctggtaagt gattgcccat tttttaacac gcttacctta ctaaacggaa tctcactttt
      421 ttctctatgc tccgcattta ctagtggagg atcaaacaca acctcaacat tataggcagg
      481 tgcgttccct gtattagcaa tgttaatatc aaagtaaaaa gcggcccaag ggtttggctc
      541 aagtgttaca actatatggg gttgagctga tgcgtcaacc attctttttg tctcgtctgc
      601 tctgaagtgg tgaagtggtt tactgaattt ggccacctga acagaggtga tatgctcacc
      661 tcagaacaac acaggtgctc caatgaaaaa aagaaatttt agcgcagagt ttaaacgcga
      721 atccgctcaa ctggttgttg accagacata cacggtggca gatgccgcca aagctatgga
      781 tgttggcctt tccacaatga caagatgggt caaacaactg cgtgatgagc gtcagggcaa
      841 aacaccaaaa gcctctccga taacaccaga acaaatcgaa atacgtaagc tgaggaaaaa
      901 gctacaacgc attgaaatgg agaatgaaat attaaaaaag gctacaaccg cgctcttgat
      961 gtcagactcc ctgaacagtt ctcgataatc aggaaactca gagcgcatta tcctgtggtc
     1021 acactctgcc atgtgttcgg ggttcatcgc agcagctaca gatactggaa aaaccgtcct
     1081 gaaaaaccag acggcagacg ggctgtatta cgcagccagg tacttgaact gcatggcatc
     1141 agccacggct ctgccggagc aagaagcatc gccacaatgg caacccggag aggctaccag
     1201 atggggcact ggcttgctgg cagactcatg aaagagctgg ggctggtcag ttgccagcag
     1261 ccgactcacc ggtataagcg tggcggtcat gagcacgttg ctatcccgaa tcatcttgag
     1321 cgacagttcg ccgtaacgga accaaatcag gtgtggtgcg gtgatgtgac ctatatctgg
     1381 acgggtaagc gctgggcgta cctcgccgtt gttctcgacc tgttcgcaag aaaaccagtg
     1441 ggctgggcca tgtcgttctc gccggacagc aggcttacca tgaaagcact ggaaatggca
     1501 tgggaaaccc gtggtaagcc cgtcggggtg atgttccagg cgatcaaggc agtcattata
     1561 cgagcaggca gttccggcag ttactgtggc gataccggat caggcagagt atgagtcggc
     1621 gtggaaactg ctgggataac agcccaatgg agcgcttctt caggagtctg aagaacgaat
     1681 gggtgccagc gacgggctat gtaagcttca gcgatgcagc tcacgcaata acggactata
     1741 tcgttggata ttacagcgca ctaagaccgc acgaatataa tggtgggtta ccaccaaacg
     1801 aatcagaaaa ccgatactgg aaaaactcta acgcggaggc cagttttagt tgaccacaac
     1861 agactacctg aagggagccg cggtcgccta gcagttgcag tagcaggaga tcatccagcc
     1921 gcagtacagg tcacgatgac tctggttaat gataccggct ttgaccccgt attttccggc
     1981 tctatcgctg aatcatggcg tcagcagccg tgcacaccat cctattgttg tgactgggag
     2041 gctgccacca tgcttcgcgc tttccctctg gcgaaaaagg gagaaggacg ggcccgtctg
     2101 ccttcacttt atgccagctt cggtaagctg ggtgagacac cgactcataa agatatcatt
     2161 gataacaatc gatccatcaa ctggcctgta taacgtggct gccggtgatt aagaaagctg
     2221 cacctaccta agtagtagca aacgcacact ttttagaaaa atcgatggtc agaaactgga
     2281 ttagcaattc cgttccaggg ttgcttttga tttacgttgg cgtctgatca ttgatttatc
     2341 ctcaaaagcc caacctcatt ggtaatgaac cagctccgtg aatgtccgct ctggcacaga
     2401 gcgaaatttt ttgatctccc cccctgaaat ctaaacttag tcatgtcacg tttttgggtt
     2461 tctaaaattt taacttcgcg tttttcgttg ccgtaagggt tatacagaaa tgtccgttaa
     2521 gcagagttca aaattgattg ccgtgatcac gactggtttg aaagccgcgc ccaagcctgt
     2581 acagctctgg tttgcgttga ttatgaacct gtcagcctaa agcaagcgga tggacgatga
     2641 gtattggtaa tctttcagag tccggaaaag ttcagcccca gtctgaacag gcttgctggc
     2701 gccagtccag tttcattcag tcgtggtttg gttcttacgg cctgtgcaat ctacctcatt
     2761 aggcacatcg gcctgccaga taccggctcg gggtgtattt ccgcttccac gctgaatact
     2821 gttctcagca atcctggggt catcacctct tctggtgtgc cttgcgccat aacatgtccg
     2881 tttgccatta ccaccagttg atcgcagtac cggctagcct gattaaggtc gtgcagcaca
     2941 gcgaccaccg ttttcccctg agtccggagt tcgcccatca accgcatcag gtccacctgg
     3001 tgattgatat caagataggt ggttggctca tcaagtaata caacgggcgt attctgggcc
     3061 aggaccatcg ccagaaatgc gcgctggcgc tgaccgccgg aaagctcggt taaccgacga
     3121 acggcaagat gattgatccg ggtctggttc atggcgacat taactcgtgc attgtcttca
     3181 gcggagagac gcccccagag tgacagccag ggattacgac catacgaaac cagctcctgg
     3241 actgtgatcc cctctggcgt taaatggtgc tgaggcagca gcgaaagcct gcgggccaac
     3301 tggcgcgatg agagcatatt tatgggatta tcgccgagaa atacggtgcc agactgcggc
     3361 attaaaagcc gcgaaaaaca gtttaacagc gtcgatttcc cgcaaccgtt aggaccgatc
     3421 agggcggtga tcttccccgt tggcagtgag agtgaaacgt cgttaagtac cttgtctgtc
     3481 ccgtaactga ccgtcagatt ttcagttcgt aaagtcattt atcgcattct cacaagcaac
     3541 cagacaaacc acggcgcacc gataatggcg gtcagcacgc caaccgggag ctccagtggg
     3601 ggatgaataa ttctcgccag cagatcggca accaccaaca gcaacgcacc tgtcagggct
     3661 gaaacaggca gcagtctgcg gtgacgtcca ccggtgatgc tacgcatcat atgcggcacc
     3721 acgagaccaa taaagctaat cgggccgcag gcggccacgc cggtagatgt catggcgaca
     3781 gctagtaaca aagcccagaa tcgggtatgg ggcaccgaca caccgagcgt ggtggcgcgc
     3841 gcatcgccga gtgcaaggag gtcgagatcg cggcaaaaac tcaggctcag cggcagaaat
     3901 aaaatcatca gcgggatggc aatcttcaca aagctccagt cacggcccca taagctgccg
     3961 gtcagccaca gcagggcgtt gttcacatcc tgtgggcgcg agagcatcag ataatccgtc
     4021 aggctggccc agcatgcaga aagcgccacg ccggtgagcg ccagcttcat cggctggtgg
     4081 gtctttgcca gcatcttcag taatatcaac cccgccatgc cgcccgcaaa ggccagcagc
     4141 ggcagcacca tcacgggcag tgacggcata agaagtagag cccccacaga ggccaggctg
     4201 gcggcatggt taacgccgag aatatccggt gatgccagag ggttgcgcac aatcccctgt
     4261 atcagcacgc ccgccacggc gagggctgca ccgacaaaca gtgccagcag caagcgcggc
     4321 agtcggtact ccatcaatac ataataatgc tcgcgtccgg cctgccagtc ggtcagcagc
     4381 gcgcgccacg gcacggggat cactcccata tggagtgata acagcgcaca gcccgccagg
     4441 gcaagggtga tgaaaataac cagcgcaatt ttcatcctcg cctcctcaca agccagacaa
     4501 agcaagggct gccaatcagc gccagcactg cgcctgcggg cagatctccg gggaaggcca
     4561 gcgcgcgtgc gagtacatct gccagcagca tcagcgtggc ccccagcagc atgctcactg
     4621 gcagtacgtt gcgctgatcg aagcctgccc agaagcgcgc cagatgtggc accagcagac
     4681 cgataaacgc caccggacct gccacgctga cgcacgcacc aaccagaagc agcactaaca
     4741 tattgatgac caaacgtagc ctcgtcaggt tcactcccag cgtatgggcg gtgctgtcgc
     4801 tgaggttgag caggttcagt tgattcgcca gcagcaacac gacagggact gcagtgacca
     4861 ccaccggcaa gagctgccag acatcctgcc agcgggcgtg ggacactccg cctgccagcc
     4921 agtaaaagat gccgtaagca tgatcttcgg ccagcagcag ggggatgcgg gtaaggccca
     4981 tacaaaaggc cgacagcgcg atactcgcga ggatcagttt gtttctgtca tgggtatgac
     5041 gaaatccgcc tcctgcggtc atggccagca gccagctcac gccgcccccg catgccgcga
     5101 tgaacgacag agaatagcct gcaatcggcg tcggactcag cgcgctggta agcgccatag
     5161 ccagcgccgc gccgctgtta atgccgagca gtgaaggaga ggccattggg ttgtgggtca
     5221 gggtttgcag cagcgtgccc gcgagcgcca ggcttgcgcc gatcagaacg gcgaccaggc
     5281 ttcgtggcaa acgaaggttt tgcaccagcg cttctggtag cgttggcgtg tgtccaggca
     5341 gcagggcgcg ggttgcatct gctccggaaa caggaatggc cgagtagcaa aacagactca
     5401 gccagaaaat aataataagt gctgcaacgg gaagccccca cagcagcacc gggtgtttta
     5461 tcgcggtcat ttcacaacgg taagcggctg atggtggaag atttttaccg tgtcagcggc
     5521 aatacgctct gcagcaaaaa taccgcgcat ccgcgcccag gtgttactgt cgaccgaagc
     5581 aacctgctgc ttctgcgcgg cggttaacat ctgccagagc ggatcttgtt gccagcgttt
     5641 aacaatgctc tcttcgcgat agtgggcaac cagcagccag gcaggattga ccgccagcag
     5701 ttgctccagg ccgatggacg gcatggacgc acccgccatc gcagcgggaa cgttcagccc
     5761 cagagaggcc agcacgctgc cggtccaggt ctcctgagta tgcaggttga attgctgttc
     5821 gcgtgatgtg ccaaaggcca cgcgtgtccc tttgggaagc tggctggccc actgcgccat
     5881 cctctcttta tgttgttcca ggcgtgcctg catctctcgc tttttaccca ccatttcgcc
     5941 gatgatagcc gcagattgca aattttcagc gtaggtttcg ttgcgggact taagcagcag
     6001 taccggcgcg atttgctgca aggcgatgta aacccccgca tggcgactgc tgtcggcaat
     6061 gatcaggtct ggtttcagag cggcaatggc ttccaggctc ggctgcgcgc gcgttccgac
     6121 ggactgccac ggtttcaggt gcgcacgcac ttcgggcagg atgcgttttg catcgttatc
     6181 gtcggcaata ccgatcgggc tgacgtccac ggcggccagc gcatcggcga acgagagttc
     6241 cagcaccaca atccgttgtg gcgttttttc gagtgtaaac gtgccgtgtt cgtcctgaac
     6301 cgtggcggca aaggcgtggc tgatcaccag cagcaggcct gcaaaaacaa aacggataaa
     6361 tgccaacata atcacattcc agctaaaagc ccggcaagcc gggcgttaac acatcagaac
     6421 ttcaacgacc cctgcatata cagcgtgcgc ggctggcctg catagatgcc tttgttgttg
     6481 tcgtcataag agcggatgaa gtagtcctgg tcgaagatgt ttttcacacc gaatgccagg
     6541 ttcagatctg ccatctgcgg gccaaaatca tacgccacgc gtgcgcccca gagcatgaag
     6601 ccgggaatgc ggccggtact gccgtcggcg ctctctttca ccgtattggc gttatccgca
     6661 aactggctgg actggaaatc gctgttcaga ttgaacgtcc agtttcctgg cttgtagtcc
     6721 acgcccagcg tgcctttatg tttcggggag aatggtacca gattgccgta ggtgtcgcct
     6781 ttctcgcgga tttccgcgtt cacatacgca tagctggcgt agatggaaac gttatcaagc
     6841 gttggcgtta gcgtacccag atcgtaacgt gcctgcgttt ccagcccggt atggcgcgtt
     6901 ttgccacgtg cagtgacggt gtcgttggtc tggttggagt cgtactgatt gttaaagtta
     6961 atcaggaaca gccccatttc cgccgtcagc gcgccgtcgt cgtagcgggt accgagttcc
     7021 caggttcgcg ctttttccgg ttccacattg ccgctttgca cagccttgcc aatctggctg
     7081 tactgtacgg tgccgaacga gccttcagtg tttgcataaa gattccagct gtcagtcagg
     7141 tgatagagca cgttcaacgc cggaagcggt gcgttatagc tcacttcttc gtgcgtgcct
     7201 gtgatggcgt tgttctggta tgactcgata tgttcgaaac gcatacccgg cgtgatggtc
     7261 cagttgccga tgtcgatttt gtcatccaga taccaggcgt gcgcctcggt gccggaacgc
     7321 gtatcgcggt cgtaagggct tgagccggac ggcaactgcc cgctgctggt ggcggtgtag
     7381 taacgcattt catgcgttga ttcattcaaa tagcgatagc ccacgcccac ttcgtgcgcg
     7441 gaagggccga tcataaagat ctggctgtag cgtggctcaa taccgcgcac ccagtagtta
     7501 cgcggcgaga gggtgatgcg tttgccttgc tccaggtagc cgctgcgcag ggtttgggtg
     7561 tagaacccct gaatgttgaa tttatgctgg ctgtctggct ggaactggta gcccaggctc
     7621 gccagcttgc gacgacccca gaagcggtca tacgggcggg tggattgcca gcgatcggcg
     7681 tcgtaatccg cgcgagacag gccaccgggc atgtcggctt caccatcgta atattgcagc
     7741 aggctgttga aggtgtgcac ctcatccggc gcatatttgc ttttcagcat caggtcgtcg
     7801 atgcgggtgg cgctgtgctc gcgccagtca ctgccgcgcg tgccggagta gagcagcgcg
     7861 gtgccaaaac cgttgtccgc tgtgccgccc accatcaggt tgtgcgtctc tttcgggttg
     7921 ttttgtgaag aggttgggct gagctgacct tccacgcccg cctcgatacc aaagtcctgc
     7981 ggaatggcac gggtaacaaa gttcaccacg ccgcccacgc tctgcggtcc gtaacgcacc
     8041 gcaccaccac cgcgtaccac gtcaatggca tccatgttgc cgagcgaaac gggagccagt
     8101 gaaagctgcg gctgaccgta aggggcgaag gggacgggga tgccgtccat caggacggtc
     8161 gagcggctgg cgaggcgcgg gctcaggccc cggatgccaa agttcatcgc caggtcgtgg
     8221 ctgccggtgc cgttgttttc cggcgcgctg acgccaggga tgcggttaag tacctcacgc
     8281 atggtggttg cgccggtttt ggcgaaatcc tcacggcgga tcacgtcacg cgcgccagca
     8341 tgttcaaata cgtcgttttc acgcgcatca cccagccagt cgccgaccac ggtcagggca
     8401 tcttcttttg gtgcgggcgc gggctccagc gtccagctgt tatttcccag cggttttacc
     8461 tgcagtccgc tgccgtccag cagttgttgc aggccgctct cgacgtcgta atcgccgtgc
     8521 aggccgttgc tctgcttgcc gcgcgtcagg ctggcgtcaa ccgagagggt aaatccgctg
     8581 tgtgcggcat actgattgag cgctttatcg agcgatcccg gtgcgatatt aacctgtgca
     8641 gcaaaagcgg aaaacgagag accggccagc ggcagcaggc tcaggcgaat ggcgttaacc
     8701 aaaggtgttg ttttacgaaa aacgcgtaac ggcgtcatac cttccccatc atcatttttg
     8761 ttgtgttcag ctatgagtcg aacgagaagg gtaaaaagga caatcgaaat aagaattatt
     8821 ttccttacag tggtgaaatg tttatccagt accgcgtaat agactgaatt ttaacgggaa
     8881 gcgtttgcgc gataacgttc aggatcgcat cggtattttt cagcgggaac gtcccgctca
     8941 ggcgcagccc ggcaacggcg ggatcgcagc gcagcacgcc gttgcggtaa cgggttagcg
     9001 tggctatcac ctcacccagc ggtttatcgc tgaagctcag gatgtccttc gtccagcttg
     9061 tactctcgtc atccagcggt ttcactgcgc caaactcaga ggcgctgaac tgcaggcttt
     9121 caccagcgtt cacgatgcgt ttttgcgcgg gggcactggc gagaagcact tccacagcgt
     9181 gctgctgcac gtcaagctgc gtgaaattat cctgctggcg gacggtaaat tctgtcccta
     9241 aagcagtgag ctggccctga cgggtcagga cacggaaggg gcgttgcagg gcatctttcg
     9301 cggtagtaat ggcgatttca ccgtaccaga gccggacggt gcgctgatgc gcatcaaaac
     9361 gcacatccgc cgcgctttgg gtattcagcg tgagcaggga gccatcttcc agttgctgac
     9421 ggctgactgt gcctttggcg gtgcggtaat ctgcccgcag accttcgccg gtttccgact
     9481 gccagagctg ccagcctccg ccagcgccga gcaacagcag taatcctttc atcacgtgac
     9541 ggcgggtgag gcgggtatcg tgcaacgccc tgctggctac gtcgccaggc acaccgccaa
     9601 gctggttgcg caggttttca acctgctgcc aggcccactg gttatcctga tcctgttcat
     9661 accactgttg ccagcgcgct tcctgttgtg ggctgacgcg ctcgccgctt agcacggcat
     9721 accagtggga agctgaacgc agcgcctgac ggcgggaatc ggttaacaaa ggattcataa
     9781 cccatactcc agacggaaca gcaggcagtg ctcgacggct ttcgccacgt attttttcac
     9841 ggagctgatg gaaacaccga gtttgtgcgc aatctcgctg tatgtcagac catccagttg
     9901 cgaaagcaga aacgcttcac gtgttttgcc gtttagcccg tccagcatgc tgtcgaggag
     9961 ttgtagggtc tcgagttggc tttcgcgttc ctcaggtgaa ggcgctcccc cctccggcat
    10021 aagcgccagc atctccagat acgctttttc cagcgcgttt cggcgaaaca ggtccaccat
    10081 cacgcgtttg gcgatagtgc agaggaagga gcgaggatcg cggatcgtcg agagcgtttc
    10141 gctgaccatt acccgcaaaa aagtgtcctg ggcaatgtca tctgcatcaa aagcagactg
    10201 gagtttgcgc gtcagccagc ttttcaacca gccgtgatgt gtgccataaa gcgactcgaa
    10261 cgttaaggaa gctgtggtag tggcgcggtc agacatgcgg agtgcatcaa aagttaatta
    10321 tcacgtagtc atattaatat gagaatggtt atcattacaa ttggaaataa aattgtttcc
    10381 aatagacatt tttaacatgt tgtttttcta agtgttataa ggtaggtata aaatgggatg
    10441 gagcctctgc ttctggcatg tgtcggtcag aatgactcat gatgtggtct gctattattg
    10501 acatcctcac tgccctaaag gatgggga
//
LOCUS       DAZKHF020000157        10488 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2201S31241
            SAMN43267800-rid25106413.denovo.158, whole genome shotgun sequence.
ACCESSION   DAZKHF020000157 DAZKHF020000000
VERSION     DAZKHF020000157.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267800
            Sequence Read Archive: SRR30307263
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 10488)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10488)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 10488)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350581.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350581.2
            Genome Coverage       :: 151x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:21:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,652
            CDSs (total)                      :: 5,557
            Genes (coding)                    :: 5,320
            CDSs (with protein)               :: 5,320
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 237
            CDSs (without protein)            :: 237
            Pseudo Genes (ambiguous residues) :: 0 of 237
            Pseudo Genes (frameshifted)       :: 102 of 237
            Pseudo Genes (incomplete)         :: 130 of 237
            Pseudo Genes (internal stop)      :: 52 of 237
            Pseudo Genes (multiple problems)  :: 41 of 237
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10488
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267800-rid25106413.denovo.158"
                     /serotype="O 26:H11"
                     /isolate="2201S31241"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-01"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            69..1436
                     /gene="dcuC"
                     /locus_tag="ACHOSC_RS16095"
                     /old_locus_tag="pgaptmp_003219"
     CDS             69..1436
                     /gene="dcuC"
                     /locus_tag="ACHOSC_RS16095"
                     /old_locus_tag="pgaptmp_003219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic C4-dicarboxylate transporter DcuC"
                     /protein_id="HGU4549114.1"
                     /translation="MFGIIISVIVLITMGYLILKNYKPQVVLAAAGIFLMMCGVWLGF
                     GGVLDPAKSSGYLIVDIYNEILRMLSNRIAGLGLSIMAVGGYARYMERTGASRAMVSL
                     LSRPLKLIRSPYIILSATYVIGQIMAQFITSASGLGMLLMVTLFPTLVSLGVSRLSAV
                     AVIATTMSIEWGILETNSIFAAQVAGMKIATYFFHYQLPVASCVIISVAISHFFVQRA
                     FDKKDKNINHEQAELKALDNVPPLYYAILPVMPLILMLGSLFLAHIGLMQSELHLVAV
                     MLLSLTVTMFVEFFRKHNLRETMDDVQAFFDGMGTQFANVVTLVVAGEIFAKGLTTIG
                     TVDAVIRGAEHSGLGGIGVMIIMALVIAICAIVMGSGNAPFMSFASLIPNIAAGLHVP
                     AVVMIMPMHFATTLARAVSPITAVVVVTSGIAGVSPFAVVKRTAIPMAVGFVVNMIAT
                     ITLFY"
     gene            complement(1479..1976)
                     /gene="sspB"
                     /locus_tag="ACHOSC_RS16100"
                     /old_locus_tag="pgaptmp_003220"
     CDS             complement(1479..1976)
                     /gene="sspB"
                     /locus_tag="ACHOSC_RS16100"
                     /old_locus_tag="pgaptmp_003220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ClpXP protease specificity-enhancing factor"
                     /protein_id="HGU4549115.1"
                     /translation="MDLSQLTPRRPYLLRAFYEWLLDNQLTPHLVVDVTLPGVQVPME
                     YARDGQIVLNIAPRAVGNLELANDEVRFNARFGGIPRQVSVPLAAVLAIYARENGAGT
                     MFEPEAAYDEDTSIMNDEEASADNETVMSVIDGDKPDHDDDTHPDDEPPQPPRGGRPA
                     LRVVK"
     gene            complement(1982..2620)
                     /gene="sspA"
                     /locus_tag="ACHOSC_RS16105"
                     /old_locus_tag="pgaptmp_003221"
     CDS             complement(1982..2620)
                     /gene="sspA"
                     /locus_tag="ACHOSC_RS16105"
                     /old_locus_tag="pgaptmp_003221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="stringent starvation protein SspA"
                     /protein_id="HGU4549116.1"
                     /translation="MAVAANKRSVMTLFSGPTDIYSHQVRIVLAEKGVSFEIEHVEKD
                     NPPQDLIDLNPNQSVPTLVDRELTLWESRIIMEYLDERFPHPPLMPVYPVARGESRLY
                     MHRIEKDWYTLMNTIINGSASEADAARKQLREELLAIAPVFGQKPYFLSDEFSLVDCY
                     LAPLLWRLPQLGIEFSGPGAKELKGYMTRVFERDSFLASLTEAEREMRLGRS"
     gene            complement(3015..3407)
                     /gene="rpsI"
                     /locus_tag="ACHOSC_RS16110"
                     /old_locus_tag="pgaptmp_003222"
     CDS             complement(3015..3407)
                     /gene="rpsI"
                     /locus_tag="ACHOSC_RS16110"
                     /old_locus_tag="pgaptmp_003222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000829824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S9"
                     /protein_id="HGU4549117.1"
                     /translation="MAENQYYGTGRRKSSAARVFIKPGNGKIVINQRSLEQYFGRETA
                     RMVVRQPLELVDMVEKLDLYITVKGGGISGQAGAIRHGITRALMEYDESLRSELRKAG
                     FVTRDARQVERKKVGLRKARRRPQFSKR"
     gene            complement(3423..3851)
                     /gene="rplM"
                     /locus_tag="ACHOSC_RS16115"
                     /old_locus_tag="pgaptmp_003223"
     CDS             complement(3423..3851)
                     /gene="rplM"
                     /locus_tag="ACHOSC_RS16115"
                     /old_locus_tag="pgaptmp_003223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010847760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L13"
                     /protein_id="HGU4549118.1"
                     /translation="MKTFTAKPETVKRDWYVVDATGKTLGRLATELARRLRGKHKAEY
                     TPHVDTGDYIIVLNADKVAVTGNKRTDKVYYHHTGHIGGIKQATFEEMIARRPERVIE
                     IAVKGMLPKGPLGRAMFRKLKVYAGNEHNHAAQQPQVLDI"
     gene            complement(4070..5197)
                     /gene="zapE"
                     /locus_tag="ACHOSC_RS16120"
                     /old_locus_tag="pgaptmp_003224"
     CDS             complement(4070..5197)
                     /gene="zapE"
                     /locus_tag="ACHOSC_RS16120"
                     /old_locus_tag="pgaptmp_003224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapE"
                     /protein_id="HGU4549119.1"
                     /translation="MQSVTPTSQYLKALNEGSHQPDDVQKEAVSRLEIIYQELINSRP
                     PAPRTSGLMARVGKLWGKREDTKHTPVRGLYMWGGVGRGKTWLMDLFYQSLPGERKQR
                     LHFHRFMLRVHEELTALQGQTDPLEIIADRFKAETDVLCFDEFFVSDITDAMLLGGLM
                     KALFARGITLVATSNIPPDELYRNGLQRARFLPAIDAIKQHCDVMNVDAGVDYRLRTL
                     TQAHLWLSPLNDETRAQMDKLWLALAGAKRENSPTLEINHRPLATMGVENQTLAVSFT
                     TLCVDARSQHDYIALSRLFHTVMLFDVPVMTRLMESEARRFIALVDEFYERHVKLVVS
                     AEVPLYEIYQGDRLKFEFQRCLSRLQEMQSEEYLKREHLAG"
     gene            5391..5789
                     /gene="zapG"
                     /locus_tag="ACHOSC_RS16125"
                     /old_locus_tag="pgaptmp_003225"
     CDS             5391..5789
                     /gene="zapG"
                     /locus_tag="ACHOSC_RS16125"
                     /old_locus_tag="pgaptmp_003225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Z-ring associated protein ZapG"
                     /protein_id="HGU4549120.1"
                     /translation="MTWEYALIGLVVGIIIGAVAMRFGNRKLRQQQALQYELEKNKAE
                     LDEYREELVSHFARSAELLDTMAHDYRQLYQHMAKSSSSLLPELSAEANPFRNRLAES
                     EASNDQAPVQMPRDYSEGASGLLRTGAKRD"
     gene            5943..7310
                     /gene="degQ"
                     /locus_tag="ACHOSC_RS16130"
                     /old_locus_tag="pgaptmp_003226"
     CDS             5943..7310
                     /gene="degQ"
                     /locus_tag="ACHOSC_RS16130"
                     /old_locus_tag="pgaptmp_003226"
                     /EC_number="3.4.21.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine endoprotease DegQ"
                     /protein_id="HGU4549121.1"
                     /translation="MKKQTQLLSALALSVGLTLSASFQAVASIPGQVADQAPLPSLAP
                     MLEKVLPAVVSVRVEGTASQGQKIPEEFKKFFGDDLPDQPAQPFEGLGSGVIINASKG
                     YVLTNNHVINQAQKISIQLNDGREFDAKLIGSDDQSDIALLQIQNPSKLTQIAIADSD
                     KLRVGDFAVAVGNPFGLGQTATSGIVSALGRSGLNLEGLENFIQTDASINRGNSGGAL
                     LNLNGELIGINTAILAPGGGSVGIGFAIPSNMARTLAQQLIDFGEIKRGLLGIKGTEM
                     SADIAKAFNLDVQRGAFVSEVLPGSGSAKAGVKAGDIITSLNGKPLNSFAELRSRIAT
                     TEPGTKVKLGLLRNGKPLEVEVTLDTSTSSSASAEMITPALEGATLSDGQLKDGGKGI
                     KIDEVVKGSPAAQAGLQKDDVIIGVNRDRVNSIAEMRKVLAAKPAIIALQIVRGNESI
                     YLLMR"
     gene            7400..8467
                     /gene="degS"
                     /locus_tag="ACHOSC_RS16135"
                     /old_locus_tag="pgaptmp_003227"
     CDS             7400..8467
                     /gene="degS"
                     /locus_tag="ACHOSC_RS16135"
                     /old_locus_tag="pgaptmp_003227"
                     /EC_number="3.4.21.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane-stress sensor serine
                     endopeptidase DegS"
                     /protein_id="HGU4549122.1"
                     /translation="MFVKLLRSVAIGLIVGAILLVAMPSLRSLNPLSTPQFDSTDETP
                     ASYNLAVRRAAPAVVNVYNRGLNTNSHNQLEIRTLGSGVIMDQRGYIITNKHVINDAD
                     QIIVALQDGRVFEALLVGSDSLTDLAVLKINATGGLPTIPINARRVPHIGDVVLAIGN
                     PYNLGQTITQGIISATGRIGLNPTGRQNFLQTDASINHGNSGGALVNSLGELMGINTL
                     SFDKSNDGETPEGIGFAIPFQLATKIMDKLIRDGRVIRGYIGIGGREIAPLHAQGGGI
                     DQLQGIVVNEVSPDGPAANAGIQVNDLIISVDNKPAISALETMDQVAEIRPGSVIPVV
                     VMRDDKQLTLQVTIQEYPATN"
     gene            complement(8530..9468)
                     /gene="mdh"
                     /locus_tag="ACHOSC_RS16140"
                     /old_locus_tag="pgaptmp_003228"
     CDS             complement(8530..9468)
                     /gene="mdh"
                     /locus_tag="ACHOSC_RS16140"
                     /old_locus_tag="pgaptmp_003228"
                     /EC_number="1.1.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000861594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030060 - L-malate dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malate dehydrogenase"
                     /protein_id="HGU4549123.1"
                     /translation="MKVAVLGAAGGIGQALALLLKTQLPSGSELSLYDIAPVTPGVAV
                     DLSHIPTAVKIKGFSGEDATPALEGADVVLISAGVARKPGMDRSDLFNVNAGIVKNLV
                     QQVAKTCPKACIGIITNPVNTTVAIAAEVLKKAGVYDKNKLFGVTTLDIIRSNTFVAE
                     LKGKQPGEVEVPVIGGHSGVTILPLLSQVPGVSFTEQEVADLTKRIQNAGTEVVEAKA
                     GGGSATLSMGQAAARFGLSLVRALQGEQGVVECAYVEGDGQYARFFSQPLLLGKNGVE
                     ERKSIGTLSAFEQSALEGMLDTLKKDIALGEEFVNK"
     gene            9903..10373
                     /gene="argR"
                     /locus_tag="ACHOSC_RS16145"
                     /old_locus_tag="pgaptmp_003229"
     CDS             9903..10373
                     /gene="argR"
                     /locus_tag="ACHOSC_RS16145"
                     /old_locus_tag="pgaptmp_003229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001257852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator ArgR"
                     /protein_id="HGU4549124.1"
                     /translation="MRSSAKQEELVKAFKALLKEEKFSSQGEIVAALQEQGFDNINQS
                     KVSRMLTKFGAVRTRNAKMEMVYCLPAELGVPTTSSPLKNLVLDIDYNDAVVVIHTSP
                     GAAQLIARLLDSLGKAEGILGTIAGDDTIFTTPANGFTVKDLYEAILELFDQEL"
BASE COUNT         2558 a         2669 c         2624 g         2637 t
ORIGIN      
        1 tataacatca ttgttttcaa tctgccgttt atgggattga ccgttttctt ttgacacgga
       61 gttcaacaat gttcggcata attatatctg tcatcgtatt aattacgatg ggctatttga
      121 tcctgaaaaa ctacaaacct caggtggtgc tggctgccgc aggtatcttc ctgatgatgt
      181 gcggtgtctg gttagggttc ggtggtgtac tcgatcccgc caaaagcagc ggctacttga
      241 tcgtcgatat ttataatgaa atcctgcgca tgctgtccaa ccgcattgcc ggattggggc
      301 tgtcgattat ggcagtgggc ggttatgccc gctacatgga gcgcacagga gccagtcgcg
      361 cgatggtgag cttgctaagc cgcccgttaa aactcattcg ctcgccgtat attattctgt
      421 cggcaactta cgtcatcggc caaatcatgg cgcagtttat taccagcgcc tccggcctgg
      481 gtatgttgct gatggtcacc ttatttccga cgctggtgag tctgggagta agtcgcctct
      541 ctgcggtggc ggttatcgca accacgatgt ccattgagtg ggggattctg gaaacgaact
      601 ccatttttgc agcccaggtc gcgggaatga aaattgccac ttacttcttc cactaccagc
      661 ttccggtcgc ctcttgcgtc attatctcgg tggcgatctc ccactttttc gtgcaacgcg
      721 cttttgacaa aaaagataaa aatatcaatc acgaacaggc agagctaaaa gctctcgata
      781 atgtcccgcc gctctattac gccattttac ctgtgatgcc gttaatcttg atgctcggct
      841 cgctgttcct cgcccacatc gggctgatgc agtcagaact gcatctggtg gcggtgatgt
      901 tactgagttt gacggtgacg atgtttgttg agttcttccg caagcataac ttgcgcgaaa
      961 caatggacga tgtgcaggcg ttttttgacg gcatgggtac gcagtttgcc aacgtggtaa
     1021 cgctggtggt cgcgggtgaa atatttgcga aaggcttaac gacgattggc actgttgatg
     1081 cggttatcag gggtgcggag cattctggtc tgggcggtat tggcgtgatg attattatgg
     1141 cgctagtcat tgccatttgt gccattgtga tgggctctgg caatgcgccg tttatgtcat
     1201 ttgccagtct tattccgaat atcgcagccg gactacatgt accagcggtt gtaatgatta
     1261 tgccgatgca ttttgccacg acgctagcgc gcgcggtttc gccgattact gcggtggtgg
     1321 tcgttacgtc aggaattgca ggcgtttcgc cttttgcggt ggtgaagcga acagcgatcc
     1381 ccatggcagt cggtttcgtg gtgaatatga ttgccacaat cacgctattt tattaagcca
     1441 ttaaaagaca aaacaggcca ccagggcctg tttttgtatt acttcacaac gcgtaatgcc
     1501 ggtcgaccac cgcgtggtgg ctgcggaggt tcatcgtcag gatgagtgtc atcatcgtga
     1561 tctggcttgt cgccatcaat aaccgacata acggtttcgt tgtctgccga tgcctcttca
     1621 tcattcatga tgctggtatc ttcatcgtag gcagcttcag gctcaaacat cgtgcctgcg
     1681 ccattttcac gggcgtagat agccagcacg gcagccagcg gcacagaaac ctgacgcgga
     1741 atgccgccaa agcgcgcgtt aaagcgtacc tcatcattcg ccagttccag attgccgaca
     1801 gcacgcggcg caatgttgag tacgatttgc ccgtcacgcg catattccat aggaacctgc
     1861 acgccaggga gcgtcacatc caccaccaag tgcggcgtga gctggttatc cagcaaccac
     1921 tcatagaatg cacgcagcag atagggacga cgtggtgtta gctgtgacaa atccatacag
     1981 attaactccg gcccagacgc atttcacgtt ctgcttcagt taaagaagca aggaaagagt
     2041 cacgctcaaa gacgcgggtc atatagcctt tcagctcttt cgcacccggg ccgctgaact
     2101 cgatgcccag ttgcggcaga cgccacagca gcggagcaag atagcaatcg accaggctga
     2161 actcatcgct caggaagtac ggcttctgac cgaagaccgg cgcaatcgcc agcagttctt
     2221 cgcgcagttg cttacgtgcg gcatctgctt cagaagctga accgttgatg atggtgttca
     2281 tcagcgtgta ccagtctttt tcgatgcgat gcatgtacag acggctttca ccgcgagcta
     2341 ccgggtaaac aggcatcagt ggcggatgcg ggaaacgctc atccagatat tccataatga
     2401 tgcgagattc ccacagggtc agctcacgat ccaccagggt cggaacgctc tgattcgggt
     2461 tgaggtcaat cagatcctga ggcggattgt ccttttccac gtgttcgatc tcgaaactta
     2521 cacctttctc agccagcaca atgcggacct gatggctata gatgtcagta ggaccggaaa
     2581 acagcgtcat taccgaacgt ttgttggcag cgacagccat gaaaacctcc aggtatagtc
     2641 agaattttta ctgctaccag ccaccaggtg gccagtcaga agttgtgtta cccagtaagg
     2701 aacgactctc tttgttcgaa aatcaaacaa aaaatgagca atacccgaca tttgggcaga
     2761 aaattggatg atagtttacc agattttgcg acctttgtgg tgagtcgatg ccggaaatgg
     2821 ggaaaaagag atgcgcttta gtctgaaata gttgacttag tcccttattg gcgatgtggt
     2881 ttttgttttg cctgtctgtc agacggtagc aaaaagcaac tttccaattt ttacgctgat
     2941 tcagaattta accataaaaa aacccgccga agcgggtttt ttcgcaaatt gttttctgcc
     3001 ggagcagaag ccaattaacg tttggagaac tgcggacgac gacgtgcttt acgcagaccg
     3061 actttcttac gttcaacctg acgagcgtca cgagtaacga agccagcttt acgcagttca
     3121 gaacgcaggg actcgtcgta ttccatcaga gcgcgggtga taccgtgacg gatcgcacca
     3181 gcctgaccag agataccacc acctttaacg gtgatgtaca ggtccagttt ctcaaccatg
     3241 tcgaccagtt ccagcggctg acgaactacc atgcgagcag tttcacgacc gaagtactgt
     3301 tccagagaac gttggttgat tacgatttta ccgttgcccg gtttgatgaa aacgcgagct
     3361 gcggaacttt tgcggcgacc agtgccgtag tattgatttt cagccattgc ctataatccc
     3421 gattagatgt caagaacttg cggttgctgt gccgcgtggt tgtgctcgtt acccgcgtaa
     3481 actttcagtt tacggaacat agcacgaccc agcgggcctt ttggcaacat gcctttaacc
     3541 gcgatttcaa tcacacgctc aggacggcga gcaatcatct cttcaaaggt cgcttgtttg
     3601 ataccaccga tgtggccggt gtggtgatag tacactttgt cagtacgctt gttgccggtt
     3661 acagcaactt tgtcagcgtt cagaacgatg atgtaatcac cggtatctac gtgcggagtg
     3721 tattccgctt tgtgcttacc gcgcaggcga cgagccagtt cagtagccag acggcccaga
     3781 gttttaccgg tcgcgtcaac aacataccag tcgcgtttta cggtttctgg tttagctgta
     3841 aaagttttca ttaaaagctt acccaataaa tagttacacg ttggtgaaca cccaaacgtc
     3901 ttcaattgtt gaggttcaca cgacaaagtc cggcaaacct accccttcga atagcctatg
     3961 ccagcacaca aaaagttttg ggaaaaaaac tttcttgtaa cgtggggtcg caggattata
     4021 gagaagtcgg ggtcaaagat cgaccccttt ttgtgatttg tgacaggttt taacccgcca
     4081 aatgctcgcg cttcagatac tcttcgcttt gcatctcttg cagacgtgac aggcaacgct
     4141 ggaactcaaa cttcagccga tcgccctgat aaatttcata cagcggcact tctgcactca
     4201 ccactaattt gacatggcgc tcgtaaaact catccaccag cgcaataaag cgccgcgctt
     4261 cgctctccat caaccgcgtc ataactggta catcaaacaa catgaccgta tgaaagagac
     4321 gtgagagcgc aatatagtca tgctgactgc gggcgtcgac gcacagcgta gtaaaagaga
     4381 ccgccagcgt ctggttctcg acgcccattg tcgccaatgg ccgatggttg atttctaacg
     4441 tcggtgaatt ttctcgtttc gcccccgcca gcgccaacca tagtttatcc atctgcgccc
     4501 gggtttcatc gttgagtggc gaaagccaca gatgcgcctg agtgagtgta cgcagtcgat
     4561 aatcaacacc agcgtccacg ttcattacat cacaatgctg tttaatggca tcgattgcag
     4621 gcagaaaacg cgcacgttgc aggccatttc gataaagttc atccggcgga atatttgacg
     4681 tcgctaccag ggtaatgccg cgggcgaaca gggctttcat cagaccgcca agtagcatgg
     4741 cgtcggtaat atcagaaaca aaaaattcgt caaaacagag cacgtcagtt tcggctttaa
     4801 agcgatcggc aataatttcc agcggatcgg tctgcccctg taaggcagtt agctcttcgt
     4861 gcacccgcag cataaaacgg tgaaagtgca ggcgctgttt ccgctctccc ggcaggcttt
     4921 gatagaaaag gtccatcagc caggttttcc cgcgtcctac accgccccac atatataagc
     4981 cacgcactgg cgtatgcttt gtgtcttcgc gtttacccca cagcttaccg acccgcgcca
     5041 ttagcccact cgtcctgggg gctggtggcc tgctattgat gagttcttga taaataattt
     5101 ccaggcggct gacggcctct ttttgaacgt cgtcgggttg atggctgcct tcattgagcg
     5161 ccttcaggta ttgcgatgtt ggggtaacgc tttgcatgat cttattgtta ttccttgaat
     5221 aatcggtgcg ccgttgttca cggttgacga aaaaaaggcc gttctacact acgcgatatg
     5281 cagtcgggat tccacttctg tggaattaac ggttatagtg gcataatcag ccgcaggcat
     5341 ggagcctgaa gccaacaccc tacggaaaca aaagacaacg ggagatgttc atgacctggg
     5401 aatatgcgct aattgggtta gtcgtcggca tcattattgg tgctgtggcc atgcgttttg
     5461 gtaatcgtaa actacgccag caacaggcgt tgcagtacga actggaaaag aataaagctg
     5521 aactggacga gtatcgcgaa gagctggtta gccactttgc ccgcagcgcg gaattactgg
     5581 ataccatggc gcacgactat cgccagctgt atcagcacat ggcgaaaagc tccagcagcc
     5641 tgctgccgga actgtctgct gaagcaaacc cgttccgtaa tcgtctggca gagtctgaag
     5701 ccagcaacga tcaggcaccg gtgcagatgc ctcgcgacta ttctgaaggc gcatccggcc
     5761 tgctgcgtac tggcgcgaag cgcgactaat ttatttttcg ggcgcagcca ttgcgccctc
     5821 ctcttctctc cctccccgac tatcatttaa tctggtgtct cattgttagc cgtctgaaaa
     5881 ttcaataaca tcaaactgtt ttgaatctct tttcttatca ttcaggtacg agagcaggaa
     5941 taatgaaaaa acaaacccag ctgttgagtg cattagcgtt aagtgtcggg ttaactctct
     6001 cggcgtcatt tcaggccgtc gcgtcgattc caggccaggt tgccgatcag gcccctctcc
     6061 ccagtctggc tccaatgctg gaaaaagtgc ttccggcagt ggtgagcgta cgggtggaag
     6121 gaacggccag tcagggacag aaaatcccgg aagaattcaa aaagtttttt ggtgatgatt
     6181 taccggatca acctgcacaa cccttcgaag gtttaggttc cggtgtcatc atcaacgcca
     6241 gtaaaggcta tgtgctgacc aacaaccatg tgattaatca ggcacagaaa atcagtattc
     6301 agctcaatga tgggcgcgag tttgatgcaa aactgattgg tagcgatgac cagagcgata
     6361 tcgccctgtt acaaattcaa aacccgagca aattaacgca aatcgctatt gccgactccg
     6421 ataaattgcg cgtcggtgat tttgccgtag cggtcggtaa cccatttggc cttgggcaaa
     6481 ccgccacctc tggcattgtt tccgcattag gccgcagcgg gttgaatctt gaaggtctgg
     6541 aaaactttat ccagacagat gcttccatta accgcggtaa ctccggcggt gcactattaa
     6601 accttaacgg tgagttaatt ggcatcaaca ctgcaatcct tgcgcctggc ggcgggagcg
     6661 tcgggattgg atttgccatc cccagtaata tggcgcgaac actggcgcag cagcttatcg
     6721 actttggtga aatcaaacgc ggtttgttag gcatcaaagg caccgagatg agtgccgata
     6781 tcgccaaagc cttcaacctt gacgtgcagc gtggcgcgtt tgtcagcgaa gtgttgccag
     6841 gttctggctc ggcaaaagcg ggcgtcaaag cgggcgatat tattaccagc ctcaacggca
     6901 aaccgctgaa tagctttgct gagttgcgct ctcgtatcgc gactacagag ccgggcacga
     6961 aagtgaagct tggcctgctg cgtaacggca aaccactgga agtagaagtg acgcttgata
     7021 ccagcacctc ttcgtcggcc agcgctgaaa tgatcacgcc agcgctggaa ggtgcaacgt
     7081 tgagcgatgg tcagctaaaa gatggcggca aaggtattaa gatcgatgaa gttgtcaaag
     7141 gaagcccagc tgctcaggct ggcttgcaaa aagacgatgt gatcattggc gtcaaccgcg
     7201 atcgggtgaa ctcgattgct gaaatgcgta aagtgctggc ggcaaaaccg gccatcatcg
     7261 ccctgcaaat tgtacgcggt aatgaaagca tctatctgct gatgcgttaa tgtcgtaaac
     7321 cgggcatcag gcttacgtgt gatgtccggt taactcgtgg tatgctgctg ccgttccctt
     7381 ttttaatgac gcctccatca tgtttgtgaa gctcttacgt tccgttgcga ttggattaat
     7441 tgtcggcgct attctgctgg ttgccatgcc ttcgctgcgc agccttaacc cgctttccac
     7501 tccgcaattt gacagtaccg atgagacgcc tgccagttat aatctggcgg ttcgccgcgc
     7561 cgcgccagcg gtggttaacg tttacaaccg tggtttgaac accaactctc acaaccagct
     7621 tgagatccgc accctgggat ccggtgtaat catggatcaa cgcggttata tcatcaccaa
     7681 taaacacgtc atcaacgacg ccgatcagat catcgtcgcc ttacaggatg gacgtgtatt
     7741 tgaagcattg ctggtgggat ctgactctct aaccgatctg gcggtactta aaattaatgc
     7801 cactggcggt ttacctacca ttccgatcaa tgcacgtcgc gtaccgcaca ttggcgacgt
     7861 ggtactggcg atcggtaacc cgtacaacct cgggcagacc attacccagg ggattattag
     7921 tgctacgggt cgaatcggtc tgaacccgac cgggcggcaa aacttcctgc aaaccgatgc
     7981 ttccattaac cacggtaact ctggcggcgc gctggtgaac tcgctgggag aactgatggg
     8041 cattaacacg ctgtcgtttg ataagagtaa cgatggcgaa acgccggaag gtatcggctt
     8101 tgcgattcct ttccagttag caaccaaaat tatggataag ctgatccgcg atggtcgcgt
     8161 gatccgcggc tacattggta tcggcggacg cgagatcgca ccactgcacg cgcagggcgg
     8221 tggtatagat caactgcaag ggatcgtggt taatgaagtg tcacctgacg gcccggcggc
     8281 gaatgcgggt attcaggtca acgatctgat tatttcggtg gataacaaac cggccatctc
     8341 tgctctggag acgatggatc aggtggcaga aattcgccct ggttcggtga tcccggttgt
     8401 agtgatgcgt gatgataagc agttaacgct gcaggtcacc attcaggaat atccggcaac
     8461 caattaagtc gagtgctcaa aacaaaaaac cggagtctgt gctccggttt tttattattc
     8521 gctaattaat tacttattaa cgaactcttc gcccagggcg atatctttct tcagcgtatc
     8581 cagcatacct tccagtgcgc tctgttcaaa tgcgctcagg gtaccgatag atttacgctc
     8641 ttccacgccg tttttaccca gcagcagcgg ttgagagaag aaacgggcgt actgaccgtc
     8701 gccttcaacg taggcacatt cgacaacgcc ttgttcgccc tgcagtgcac gaaccagaga
     8761 cagaccaaaa cgtgcagctg cctggcccat agacagggtt gcagacccac caccggcttt
     8821 cgcttcaacc acttcagtac ctgcgttctg gatacgtttg gtcagatcag ccacttcctg
     8881 ctcggtaaaa ctaacgccag gaacctgtga cagcagcggc agaatggtaa caccagagtg
     8941 accgccaata accggcactt caacttcgcc tggctgtttg cctttcagtt ccgcaacaaa
     9001 agtgttggaa cgaatgatat ccagcgtggt aacgccgaac agtttgtttt tgtcataaac
     9061 accggctttt ttcagcactt cagcagcaat cgcaactgtg gtgttaaccg ggttagtgat
     9121 aataccaatg cacgctttcg ggcaggtttt cgcaacttgc tgtaccaggt ttttcacgat
     9181 gccggcgtta acgttaaaca ggtcggaacg atccataccc ggtttacgcg ctacacctgc
     9241 agagataaga acgacatctg cgccttccag cgccggagtc gcatcttcac cagaaaaacc
     9301 tttgattttc acagcagtag ggatatggct cagatcgaca gccacaccgg gagtcactgg
     9361 agcgatatca tacagagaga gttctgaacc tgaaggcagt tgggttttta acagtagtgc
     9421 aagcgcctgg ccaataccgc cagcagcgcc gaggactgcg actttcatcc taaactcctt
     9481 attatattga taaactaaga tatgttgctc cgctgccgcg accttaatcc acaaaattgc
     9541 cgtttacaat gaccacatct caagaatgtg tagtcacgca agtttagcgt ttatgcattt
     9601 aattgccgta atcaggaacc taacttacgt aattaacagt cgcttatgca ttagcgcaac
     9661 attccaacag gtggtgacaa tataccctac cgttcagcca aaacaacatc aatttgataa
     9721 caattaattt acttttaaac agaatttgca tgccgtgacg caggcatgtt tctcaataac
     9781 gaaatttgat aaaatcccgc tctttcataa cattatttca gccttcttca gggctgactg
     9841 tttgcataaa aattcatctg tatgcacaat aatgttgtat caaccaccat atcgggtgac
     9901 ttatgcgaag ctcggctaag caagaagaac tagttaaagc atttaaagca ttacttaaag
     9961 aagagaaatt tagctcccag ggcgaaatcg tcgccgcgtt gcaggagcaa ggctttgaca
    10021 atattaatca gtctaaagtc tcgcggatgt tgaccaagtt tggtgctgta cgtacacgca
    10081 atgccaaaat ggaaatggtt tactgcctgc cagctgaact gggtgtacca actacctcca
    10141 gtccattgaa gaatctggtg ctggatatcg actacaacga tgcagttgtc gtgattcata
    10201 ccagccctgg cgcggcgcag ttaattgctc gcctgctgga ctcactgggc aaagcagaag
    10261 gtattctggg caccatcgct ggcgatgaca ccatctttac cacccctgct aacggtttca
    10321 ccgtcaaaga cctgtacgaa gcaattttag agctgttcga ccaggagctt taatctctgc
    10381 cccgtcgctt ctggcgtcgg ggaaaatgtt gcttatccct ctcattcccc ttctttctcc
    10441 tgcgatacat ttaacgaata gtgcgtttta ccgcgacaca tcattcac
//
LOCUS       DAZKHF020000158        10487 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2201S31241
            SAMN43267800-rid25106413.denovo.159, whole genome shotgun sequence.
ACCESSION   DAZKHF020000158 DAZKHF020000000
VERSION     DAZKHF020000158.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267800
            Sequence Read Archive: SRR30307263
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 10487)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10487)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 10487)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350581.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350581.2
            Genome Coverage       :: 151x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:21:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,652
            CDSs (total)                      :: 5,557
            Genes (coding)                    :: 5,320
            CDSs (with protein)               :: 5,320
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 237
            CDSs (without protein)            :: 237
            Pseudo Genes (ambiguous residues) :: 0 of 237
            Pseudo Genes (frameshifted)       :: 102 of 237
            Pseudo Genes (incomplete)         :: 130 of 237
            Pseudo Genes (internal stop)      :: 52 of 237
            Pseudo Genes (multiple problems)  :: 41 of 237
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10487
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267800-rid25106413.denovo.159"
                     /serotype="O 26:H11"
                     /isolate="2201S31241"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-01"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            complement(185..397)
                     /gene="cspH"
                     /locus_tag="ACHOSC_RS16150"
                     /old_locus_tag="pgaptmp_003230"
     CDS             complement(185..397)
                     /gene="cspH"
                     /locus_tag="ACHOSC_RS16150"
                     /old_locus_tag="pgaptmp_003230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold shock-like protein CspH"
                     /protein_id="HGU4549125.1"
                     /translation="MSRKMTGIVKTFDRKSGKGFIIPSDGRKEVQVHISAFTPRDAEV
                     LIPGLRVEFCRVNGLRGPTAANVYLS"
     gene            683..895
                     /gene="cspG"
                     /locus_tag="ACHOSC_RS16155"
                     /old_locus_tag="pgaptmp_003231"
     CDS             683..895
                     /gene="cspG"
                     /locus_tag="ACHOSC_RS16155"
                     /old_locus_tag="pgaptmp_003231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold shock protein CspG"
                     /protein_id="HGU4549126.1"
                     /translation="MSNKMTGLVKWFNADKGFGFITPDDGSKDVFVHFTAIQSNEFRT
                     LNENQKVEFSIEQGQRGPAAANVVTL"
     gene            906..1094
                     /gene="ymcF"
                     /locus_tag="ACHOSC_RS16160"
                     /old_locus_tag="pgaptmp_003232"
     CDS             906..1094
                     /gene="ymcF"
                     /locus_tag="ACHOSC_RS16160"
                     /old_locus_tag="pgaptmp_003232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HGU4549127.1"
                     /translation="MTQHLHFRCPCCHGSQYRTSAFDVTERNPLGAKCIFCKSTMITF
                     DNVALQIRTDHAPLDFTK"
     gene            1069..1299
                     /gene="ymcE"
                     /locus_tag="ACHOSC_RS16165"
                     /old_locus_tag="pgaptmp_003233"
     CDS             1069..1299
                     /gene="ymcE"
                     /locus_tag="ACHOSC_RS16165"
                     /old_locus_tag="pgaptmp_003233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YmcE"
                     /protein_id="HGU4549128.1"
                     /translation="MRRWISQNNIRLPRGAFFISALFFFNAVCIVSDNLLIIESFGEM
                     AYNISYLTRVPGTNTLLACCCLSRPEEVNSEY"
     gene            1289..1462
                     /gene="gnsA"
                     /locus_tag="ACHOSC_RS16170"
                     /old_locus_tag="pgaptmp_003234"
     CDS             1289..1462
                     /gene="gnsA"
                     /locus_tag="ACHOSC_RS16170"
                     /old_locus_tag="pgaptmp_003234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="addiction module toxin, GnsA/GnsB family"
                     /protein_id="HGU4549129.1"
                     /translation="MNIEELKKQAETEIADFIAQKIAELNKNTGKEVSEIRFTAREKM
                     TGLESYDVKIKIM"
     gene            complement(1510..2583)
                     /gene="yccM"
                     /locus_tag="ACHOSC_RS16175"
                     /old_locus_tag="pgaptmp_003235"
     CDS             complement(1510..2583)
                     /gene="yccM"
                     /locus_tag="ACHOSC_RS16175"
                     /old_locus_tag="pgaptmp_003235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HGU4549130.1"
                     /translation="MAEKKRTRWQRRPGTTGGKLPWNDWRNATTWRKATQLLLLAMNI
                     YIAITFWYWVRYYETASSTTFVARPGGIEGWLPIAGLMNLKYSLTTGQLPSVHAAAML
                     LLVAFIVISLLLKKAFCSWLCPVGTLSELIGDLGNKLFGRQCVLPRWLDIPLRGVKYL
                     LLSFFLYIALLMPAQAIHYFMLSPYSVVMDVKMLDFFRHMGTATLISVTVLLIASLFI
                     RHAWCRYLCPYGALMGVVSLLSPFKIRRNAESCIDCGKCAKNCPSRIPVDKLIQVRTV
                     ECTGCMTCVESCPVASTLTFSLQKPAANKKAFALSGWLMTLLVLGIMFAVIGYAMYAG
                     VWQSPVPDELYRRLIPQAPMIGH"
     gene            complement(2655..5399)
                     /gene="torS"
                     /locus_tag="ACHOSC_RS16180"
                     /old_locus_tag="pgaptmp_003236"
     CDS             complement(2655..5399)
                     /gene="torS"
                     /locus_tag="ACHOSC_RS16180"
                     /old_locus_tag="pgaptmp_003236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415513.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TMAO reductase system sensor histidine
                     kinase/response regulator TorS"
                     /protein_id="HGU4549131.1"
                     /translation="MNLTLTRRLWMGFALMALLTLTSTLVGWYNLRFISQVEKDNTQA
                     LIPTMNMARQLSEASAWELFAAQNLTSADNEKMWQAQGRMLTAQSLKINALLQALREQ
                     GFDTTAIEQQEQEISRSLRQQGELVGRRLQLRQQQRQLSQQIVAAADEIARLAQGQAN
                     NATTSAGATQAGIYDLIEQDQRQAAESALDRLIDIDLEYVNQMNELRLSALRVQQMVM
                     NLGLEQIQKNAPTLEKQLNNAVKILQRRQIRIEDPGVRAQVATTLTTVSQYSDLLALY
                     QQDSEISTHLQTLAQNNIAQFAQFSSEVSQLVDTIELRNQHGLAHLEKASARGQYSLL
                     LLGIVSLCALILILWRVVYRSVTRPLAEQTQALQRLLDGDIDSPFPETAGVRELDTIG
                     RLMDAFRSNVHALNRHREQLAAQVKARTAELQELVIEHRQARAEAEKASQAKSAFLAA
                     MSHEIRTPLYGILGTAQLLADNPALNAQRDDLRAITDSGESLLTILNDILDYSAIEAG
                     GKNVSVSDEPFEPRPLLESTLQLMSGRVKGRPIRLATAIADDMPCALMGDPRRIRQVI
                     TNLLSNALRFTDEGYIILRSRTDGEQWLVEVEDSGCGIDPAKLAEIFQPFVQVSGKRG
                     GTGLGLTISSRLAQAMGGELSATSTPEVGSCFCLRLPLRVATAPVPKTVNQAVRLDGL
                     RLLLIEDNPLTQRITVEMLNTSGAQVVAVGNAAQALETLQNSEPFAAALVDFDLPDVD
                     GITLARQLAQQYPSLVLIGFSAHVIDETLRQRTSSLFRGIIPKPVPREVLGQLLAHYL
                     QLQVNNDQSLDVSQLNEDAQLMGTEKIHEWLVLFTQHALPLLDEIDIARASQDSEKIK
                     RAAHQLKSSCSSLGMRIASQLCAQLEQQPLSAPLPHEEITRSVADLEAWLNKKDLNAI
                     "
     gene            complement(5494..6567)
                     /locus_tag="ACHOSC_RS16185"
                     /old_locus_tag="pgaptmp_003237"
     CDS             complement(5494..6567)
                     /locus_tag="ACHOSC_RS16185"
                     /old_locus_tag="pgaptmp_003237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000533661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HGU4549132.1"
                     /translation="MGRRRSHERRDLPPNLYIRNNGYYCYRDPRTGKEFGLGRDRRIA
                     ITEAIQANIELLSDSGRKSLIDRIKGGDAITLHVWLDRYERILTERGIRPKTLLDYAS
                     KIRAIRRKLPDKPLTDISTKEVAAMLNTYVAEGKAASAKLIRSTLVDVFREAIAEGHV
                     ATNPVTATRTAKSEVRRSRLTANEYVEIYHAAEPLPIWLRLAMDLAVVTGQRVGDLCR
                     MKWSDINDNHLHIEQSKTGAKLAIPLTLTIDALNISLADTLQKCREASSSETIIASKH
                     HDPLSPKTVSKYFTKARNASGLSFDGNPPTFHELRSLSARLYRNQIGDKFAQRLLGHK
                     SDLMAARYRDSRGREWDKIEIDK"
     gene            complement(6545..6763)
                     /locus_tag="ACHOSC_RS16190"
                     /old_locus_tag="pgaptmp_003238"
     CDS             complement(6545..6763)
                     /locus_tag="ACHOSC_RS16190"
                     /old_locus_tag="pgaptmp_003238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001325331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excisionase"
                     /protein_id="HGU4549133.1"
                     /translation="MYLTLQEWNARQRRPRSLETVRRWVRECRIFPPPVKDGREYLFH
                     ESAVKVDLNRPVTGSLLKRIRNGKKAKS"
     gene            complement(6803..6970)
                     /locus_tag="ACHOSC_RS16195"
                     /old_locus_tag="pgaptmp_003239"
     CDS             complement(6803..6970)
                     /locus_tag="ACHOSC_RS16195"
                     /old_locus_tag="pgaptmp_003239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4549134.1"
                     /translation="MHFRVTGELNGEPFNRVIEAEDINDCYDHWMIWAQIAHADVTNI
                     RIEELKEHQAA"
     gene            complement(7006..7305)
                     /locus_tag="ACHOSC_RS16200"
                     /old_locus_tag="pgaptmp_003240"
     CDS             complement(7006..7305)
                     /locus_tag="ACHOSC_RS16200"
                     /old_locus_tag="pgaptmp_003240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309189.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4549135.1"
                     /translation="MACSTFNPLTLQKYQPDPEDLCSLCGGNHGKAAMIECKDKIHIC
                     LNCVDVLVDIKNEREDKKRSEAIRALDSWMRDGYSAAQIYDLAISKGEIPGVRIE"
     gene            complement(7463..7741)
                     /locus_tag="ACHOSC_RS16205"
                     /old_locus_tag="pgaptmp_003241"
     CDS             complement(7463..7741)
                     /locus_tag="ACHOSC_RS16205"
                     /old_locus_tag="pgaptmp_003241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016063072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4752 family protein"
                     /protein_id="HGU4549136.1"
                     /translation="MDSFAKYTIIDWIAFLQVLLIWFYMAYRSGQWIVSVACSKGWRW
                     WNRKNKKALALDSFYEAFNLNSLQPGYVIVVTTQSGMTIQIHKPKEEK"
     gene            complement(7741..8328)
                     /locus_tag="ACHOSC_RS16210"
                     /old_locus_tag="pgaptmp_003242"
     CDS             complement(7741..8328)
                     /locus_tag="ACHOSC_RS16210"
                     /old_locus_tag="pgaptmp_003242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001429285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF551 domain-containing protein"
                     /protein_id="HGU4549137.1"
                     /translation="MTISTKKPLYAEVHNVPDDYEFTDEELNRIIAGDMFTPRQAAIM
                     AREIQKLRAAMLHGAEPVSQTYKSQHTQFEQVADLYEMQFDDGRTCAFHTDAQKAAQW
                     LQACDGNRVQEYVKLERLRNALSGNSPVTPDGWICCSERMPDKLIPVMVMYEDGEMWS
                     AMWNGNRWDDGTEYPDPHSVTHWREMPAAPQQEVK"
     gene            complement(8325..8942)
                     /locus_tag="ACHOSC_RS16215"
                     /old_locus_tag="pgaptmp_003243"
     CDS             complement(8325..8942)
                     /locus_tag="ACHOSC_RS16215"
                     /old_locus_tag="pgaptmp_003243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001375782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4549138.1"
                     /translation="MTTITKERLLTIKQWRETYGPGSNVVLPAEEAEELARIALASLE
                     AEPIGEVSEKRLGLVMDGTVDLGGKSTYRIIKGEKAMKLLPLGTKFYTAPPAPIALEA
                     IENAIEYIRSIAFHIDEDDYHGKHIAYFMRQALAWLEGHSCSDDSQGKSDNSPLPRYQ
                     VIELTMLVKQLVSQLKKAKPDCKLPDRAMDYLLRNGLVSAEDVLR"
     gene            complement(8946..9134)
                     /locus_tag="ACHOSC_RS16220"
                     /old_locus_tag="pgaptmp_003244"
     CDS             complement(8946..9134)
                     /locus_tag="ACHOSC_RS16220"
                     /old_locus_tag="pgaptmp_003244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000060377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4549139.1"
                     /translation="MSLTVRQLISKLRKMPPEAVVVWQGYDQSEGEYNDFVGHVADVT
                     DENAPSFDPEVRVVALRG"
     gene            complement(9136..9804)
                     /locus_tag="ACHOSC_RS16225"
                     /old_locus_tag="pgaptmp_003245"
     CDS             complement(9136..9804)
                     /locus_tag="ACHOSC_RS16225"
                     /old_locus_tag="pgaptmp_003245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000034208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ead/Ea22-like family protein"
                     /protein_id="HGU4549140.1"
                     /translation="MSLIDYQALRAKAEKATCGVWSLEYGESRFDCDDALIHREVVGY
                     LPICRIEGAHPESGFDEDFQMEQQANAEFIAAANPATVLALLDELERNQQYIKRRDQE
                     NEDIALTVGKLRVELEGKDKLIAELGKQCAEWERKALSNFEECAAMAERIEEMSKQSC
                     EARERDLFESWVMHSICISKSTLEGLRTETGYRNATLSGTDFNRMWKQWKSIRATGIR
                     IKGE"
     gene            complement(10281..10388)
                     /locus_tag="ACHOSC_RS16230"
                     /old_locus_tag="pgaptmp_003246"
                     /pseudo
     CDS             complement(10281..10388)
                     /locus_tag="ACHOSC_RS16230"
                     /old_locus_tag="pgaptmp_003246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416863.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(10385..10487)
                     /locus_tag="ACHOSC_RS16235"
                     /old_locus_tag="pgaptmp_003247"
                     /pseudo
     CDS             complement(10385..10487)
                     /locus_tag="ACHOSC_RS16235"
                     /old_locus_tag="pgaptmp_003247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015980168.1"
                     /note="internal stop; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="DUF2737 family protein"
BASE COUNT         2531 a         2684 c         2359 g         2913 t
ORIGIN      
        1 tggtaatata gtttcaagtg gtactatagc tggctgttaa atggcattta taactattag
       61 gtgcagagat attcgcttaa atggattagt ttctttgtgg aaaagtaact ggtgttataa
      121 tgtatgatgt tgactattgc actattccaa taaaaccagt cagctttaaa caagcagcgt
      181 catattaaga gagataaaca tttgccgctg ttggtcctcg caggccattt acgcggcaaa
      241 attccacacg taatcctggt ataagcactt ctgcgtcgcg gggagtgaat gcggaaatat
      301 ggacctgaac ttctttacga ccgtcggagg ggataatgaa tcctttgccg cttttgcgat
      361 caaaggtttt gacaattcct gtcattttac gggacaaaca aattccttac tgaaaatact
      421 gcgctgcact atacggggtt aataaaataa agccagcgat atttaagacc gccggacggc
      481 taaaataaaa tttgcttaat ctcaattatc atgcgttaat agctgcgtcg gtttgaaaga
      541 cagacagcat acaaagtagt ttactaaagc agttctcatt atcaggcatt atccccttct
      601 tttgagtctc tctcctgaac actaagtagt ttctgtatta aagccctgtt tgccgaaagg
      661 cccaaaatga aggaagtaaa atatgtctaa taaaatgact ggtttagtaa aatggtttaa
      721 cgcagataaa ggttttggct ttatcactcc tgatgatggc agcaaagacg ttttcgtcca
      781 tttcactgcc atccagagca atgaattccg cacgctgaac gaaaatcaga aagttgaatt
      841 ttctattgag caggggcaac gtggccccgc ggcagcgaac gttgttacgc tctaaggttg
      901 ccgttattac tcaacatctc catttccgct gtccatgttg tcatggttca cagtaccgta
      961 catcggcatt cgatgtgacg gagcgaaacc ctttgggcgc taagtgtatt ttttgtaaat
     1021 cgacgatgat cacctttgat aacgtcgcgc tgcaaatacg cacggaccat gcgccgctgg
     1081 atttcacaaa ataatatcag gctccctcgt ggagcctttt ttatatctgc cttatttttc
     1141 ttcaacgctg tatgtatagt aagcgataac ctgttgatta ttgaatcttt cggggagatg
     1201 gcttataaca tttcttacct gaccagggta ccgggaacca acaccttact ggcgtgttgc
     1261 tgtctttcaa gaccagaaga ggttaacagt gaatattgaa gagttaaaaa aacaagccga
     1321 aacggaaatc gccgacttta tcgcgcaaaa aatcgccgag ctgaacaaga atacagggaa
     1381 agaagtctct gaaattcgct tcaccgcacg agaaaaaatg accgggcttg aaagttatga
     1441 tgtcaaaatc aaaataatgt gattttgtga acatcacccg tgcgaggtga tgttccgctt
     1501 gttgctaatt tagtgaccaa tcattggcgc ttgtggaatt aagcgtcggt acaattcatc
     1561 cggtaccggg ctttgccata ctcccgcata cattgcgtaa ccaatcaccg caaacataat
     1621 ccccagaacc agtagcgtca ttaaccagcc agacaacgca aaggcttttt tatttgccgc
     1681 aggtttttgc agtgaaaagg tcaatgttga ggctaccgga catgactcta cgcaagtcat
     1741 acagccggta cattccactg ttcgtacctg aattaattta tcgaccggga tccgtgatgg
     1801 gcaatttttt gcgcatttgc cacagtcgat acaactttcg gcattgcgac gaatcttaaa
     1861 cggcgacaat agcgaaacca cgcccatcag cgcgccatat gggcaaagat aacgacacca
     1921 ggcatggcga ataaacaggc tggcaatcag caaaacggtc acgctgatta atgtcgcggt
     1981 ccccatatga cgaaagaaat cgagcatttt aacgtccatc accacgctgt agggcgacaa
     2041 cataaaatag tgaatcgcct gagcgggcat caataatgcg atatagagaa aaaaactcaa
     2101 cagcaaatac ttcacgccgc gcagaggaat atccagccag cggggaagga cacattgccg
     2161 accaaacagt ttgttaccga gatcgccgat taattcagaa agcgtaccaa ccgggcataa
     2221 ccatgagcaa aaggcctttt tgagtaatag actgatgacg ataaaagcga ccaataacag
     2281 catcgcggcg gcgtggacgg acggtaactg acctgttgta aggctatatt tcagattcat
     2341 cagaccggca atcggtagcc agccttcgat acctcccggt ctggcgacaa atgtcgtgct
     2401 acttgccgtt tcgtaatagc gcacccaata ccagaacgtg atggcaatat aaatattcat
     2461 tgccaacagt aataattgcg tcgctttacg ccaggtcgtg gcattgcgcc agtcattcca
     2521 cggtaatttg ccgcccgtcg tgcctggccg ccgctgccag cgggttcttt ttttctctgc
     2581 catgattttg ccagtccgtt aagttgcata ccaaatgcca ctattctagt tgttcttaac
     2641 tggctgatat tgattcaaat cgcgttcagg tctttcttat tcaaccatgc ttccagatcg
     2701 gcaacactgc gtgtaatttc ttcgtgtgga aggggggcag ataatggctg ctgctccagt
     2761 tgtgcgcata gctggctggc gatacgcatt cccaaactcg agcaactgct ttttagctga
     2821 tgcgcggcac gctttatttt ctcgctatcc tgactggcgc gggcaatgtc gatttcatcg
     2881 agaagcggca gggcatgttg tgtaaataat accagccatt cgtggatctt ctccgtcccc
     2941 attaactgag catcttcatt gagttgcgat acatccagcg attgatcgtt attgacttgc
     3001 agttggagat agtgcgccag taactgaccg agcacttcac gcggcaccgg tttagggata
     3061 atcccgcgga ataatgaact ggtacgctgg cgcagcgttt cgtcaatgac atgggcgcta
     3121 aagccaatca aaaccagcga cggatattgc tgtgccagtt gtcgggcaag cgtaatgccg
     3181 tcgacatccg gcagatcaaa atccaccagt gcggcagcaa acggttcgct attttgcagt
     3241 gtctctaaag cctgcgcggc attgccaaca gcaacaacct gcgcaccact ggtgttcagc
     3301 atctcaacgg taattcgctg ggttagcggg ttatcttcaa ttaacagcaa acgtaaaccg
     3361 tcaagacgca ccgcctgatt gactgttttt ggcacgggtg ccgtggcaac acgtaacggc
     3421 aagcgtaaac aaaaacagct tccaacctcc ggcgtgctgg tggcgctcag ttcgccgccc
     3481 atcgcctggg ccagacggct actgatagtc agccccagcc cggtgccgcc gcgtttgccg
     3541 cttacctgca caaatggctg gaagatttct gccagtttcg cgggatcaat accgcagccg
     3601 ctgtcttcca cttcgaccag ccattgctcg ccatcagtgc gactacgcag gataatgtac
     3661 ccttcgtcag taaaacgcag ggcgttgctc aacaggttgg ttataacctg acgaatacgt
     3721 cgtggatcgc ccattaacgc gcacggcata tcatcggcaa ttgccgttgc caggcgaatc
     3781 gggcgacctt tcacccgtcc gctcattaat tgcagggtac tttccagcag cgggcgcggt
     3841 tcaaagggct catcgctgac cgaaacattc ttgccacctg cttcgatagc ggaataatcg
     3901 agaatatcgt tgaggatggt cagcaacgat tcaccagagt cagtaattgc ccgcaaatca
     3961 tcacgctggg cgttaagtgc ggggttatct gccagcagtt gagcagtgcc gagaataccg
     4021 tacagcggtg tgcggatctc atggctcatc gccgccagaa acgccgattt tgcctggctg
     4081 gctttttctg cttccgcccg tgcctgtcgg tgttctatca ccagttcctg caattcagct
     4141 gtacgcgctt tgacctgcgc cgccagctgt tcgcggtggc gattcagtgc atgaacattg
     4201 ctgcgaaacg catccatcag ccgcccgatg gtatccagct cccgtacgcc agcggtttcc
     4261 gggaaagggg agtcaatatc accgtccagc agccgttgca gcgcctgcgt ttgttcggca
     4321 agtggacgcg tgactgagcg ataaaccacg cgccagagga tcagaatcag tgcgcaaaga
     4381 gaaactattc ccagcaataa caggctgtat tgcccgcgtg cactcgcttt ttccagatgc
     4441 gccagtccgt gctgattacg cagctcaatg gtatcgacca gctgactgac ttcgctacta
     4501 aactgcgcga actgggcgat gttattttgg gcgagagttt gcaggtgagt gctgatttca
     4561 ctgtcctgct gatacagcgc cagtagatcg ctatattggc taacggtagt taacgttgtt
     4621 gcgacctgcg cacgaactcc cggatcttca atgcgtattt gccgacgttg cagaattttc
     4681 accgcattat tgagctgctt ttccagcgtt ggtgcatttt tctggatctg ctccagcccc
     4741 agattcatca ccatttgctg cacccgcaga gcgctaaggc gcagttcatt catctggtta
     4801 acatactcaa gatcgatatc aatcagccga tcgagtgcac tttcagcagc ctgacgctga
     4861 tcttgttcga tcaaatcgta aatcccggcc tgggtcgctc cagcggaagt tgtcgcatta
     4921 ttcgcctgac cttgcgccag gcgtgcgatc tcatcggcgg cagcgactat ctgctgactg
     4981 agttgccgtt gttgctggcg tagttgcaga cgccgcccca ccagttcccc ttgctgacgt
     5041 aatgaacggg agatctcctg ctcctgttgt tcaatagcgg tggtatcaaa accttgttcc
     5101 cgtaacgctt gcagcaacgc attaatcttc aggctttgtg cggtgagcat tcgcccctgc
     5161 gcctgccaca tcttttcgtt atcggcactg gtcaggttct gcgcggcgaa aagttcccag
     5221 gcgctggctt cgctcaactg gcgcgccata ttcatggtag gaatcaatgc ctgagtgttg
     5281 tctttttcca cctggctgat aaagcgcagg ttgtaccatc ccaccagggt actggtcagg
     5341 gttaacagcg ccatcagggc aaagcccatc cagagtcttc gggtcagggt taaattcacg
     5401 gtcggtgcac ttaaggtgaa taagttgtat atttaaaaac tcttttatta tcaatgaatt
     5461 aatgtaagtg ggtcattatt agtcaaaata aaatcatttg tcgatttcaa ttttgtccca
     5521 ttcccgtcca cggctgtccc tataccgcgc cgccattaaa tctgatttat gcccgagaag
     5581 acgttgagca aacttatcgc caatctggtt ccggtatagc ctcgctgaca ggctacgcag
     5641 ttcatggaat gttggcgggt ttccatcaaa tgagagtcca gatgcatttc tcgcctttgt
     5701 aaaatacttt gatactgttt tcggggaaag cggatcgtga tgctttgatg cgattatagt
     5761 ttcactgctg ctggcctccc tgcatttctg tagtgtatca gccaatgaga tattgagcgc
     5821 gtcaatcgtt agcgttagcg gaatggcgag tttagcccct gttttactct gttcaatgtg
     5881 aagatggttg tcgtttatgt ctgaccattt cattctgcac aaatcgccga ctctctgccc
     5941 tgtaacgacg gccaaatcca tcgccagcct tagccagata gggagaggtt cggctgcatg
     6001 gtaaatctcg acatactcat tagctgtcag ccttgagcgc cttacttctg actttgctgt
     6061 acgggttgct gttaccggat tcgttgcaac atgcccctcg gctattgctt cacgaaaaac
     6121 gtcaacaagg gttgacctga ttaattttgc ggaagctgct ttaccttctg ccacgtaggt
     6181 gtttagcatt gctgccactt ctttcgttga tatgtcagtg agcggtttgt ccggcaattt
     6241 tcttcggatt gccctgattt tgctggcgta gtcgagtaga gttttcggcc tgatccctct
     6301 ttcggtgagg attctttcat atcggtcaag ccacacatga agagtgattg cgtcaccgcc
     6361 tttaattctg tctatcagtg atttgcgtcc gctgtctgag agtaactcaa tattggcctg
     6421 tattgcttca gtgattgcta tcctcctgtc tcggcctaat ccaaactctt tacccgtcct
     6481 tgggtccctg tagcagtaat atccattgtt tcttatataa aggttagggg gtaaatcccg
     6541 gcgctcatga cttcgccttc ttcccatttc tgatcctctt caaaaggcta cctgttactg
     6601 gtcgatttaa gtcaaccttt accgctgatt cgtggaacag atactctctg ccatccttca
     6661 ccggaggagg gaatatcctg cattcgcgca cccatcgacg aactgtttca aggcttcttg
     6721 ggcgtcgctg gcgagcgttc cactcctgaa gtgtcaagta catcgcaaag tctccgcaat
     6781 tacacgcaat aaaaaaccgc catcaggcgg cttggtgttc tttcagttct tcaattcgaa
     6841 tattggttac gtctgcatgt gctatctgcg cccatatcat ccagtggtca tagcagtcgt
     6901 tgatgtcctc tgcttcgata actctgttga atggttctcc attcaattca cctgtgactc
     6961 ggaagtgcat ttgtgatttc cccaaaagat gcttgagtgc gcttcttatt cgattcgcac
     7021 acctggtatt tcgccttttg aaatggctaa gtcataaatt tgcgcagcac tatacccatc
     7081 tcgcatccat gaatctaagg cgcgaatagc ctcgctacgc tttttatctt ctctttcatt
     7141 tttgatatca acgaggacat caacgcaatt aaggcatatg tggattttgt ccttacattc
     7201 gatcatggca gctttaccat gatttccgcc acacagtgag cataaatctt cagggtctgg
     7261 ctggtatttc tgtaacgtta aagggttgaa tgttgaacag gccataatca tctccataaa
     7321 acaaaactcg ccgtagcgag ttcagataaa agaaatcccc gtcagtgcga ggattgttat
     7381 tgtcttttct tcgtgcattt gtcgcacttt cggcaccatc cagataggca catccgtccg
     7441 caattaacac atataggcca catcattttt cctcttttgg tttatgaatc tgaatggtca
     7501 tgccgctttg agtggtgact acaatgacat aaccaggctg aagactgtta agattgaatg
     7561 cttcgtaaaa cgaatccaag gccagcgctt ttttattctt tcggttccac caacgccatc
     7621 ccttgctaca ggctacactg acaatccact gcccactcct gtaagccata taaaaccaga
     7681 tgagcaaaac ctgaaggaat gctatccagt caataatcgt atatttcgcg aaggagtcca
     7741 tcacttcacc tcctgctgcg gtgctgctgg catttcacgc cagtgcgtaa ctgagtgcgg
     7801 atccggatat tcggtgccat catcccagcg attgccattc cacattgcag accacatctc
     7861 accgtcttca tacatgacca ttaccggaat taacttatcc ggcattcgct cactacagca
     7921 tatccaacca tccggagtta ccggagagtt gcccgatagc gcattacgca atcgctccag
     7981 cttcacgtat tcctgaaccc tgtttccgtc gcatgcctga agccattgcg cagccttttg
     8041 cgcatcagtg tgaaaggcac aagtgcgacc gtcatcaaat tgcatttcgt agaggtcagc
     8101 aacttgttca aactgcgtat gttgtgactt gtaagtttgg cttacaggtt cggcaccatg
     8161 aagcatggcg gcgcggagtt tctgtatctc ccgtgccatt attgcggcct gacgaggagt
     8221 gaacatatca cctgcaataa ttctgtttag ttcctcgtca gtgaactcat aatcatcagg
     8281 aacattgtga acctcagcat aaagaggctt ttttgtagaa atggtcatcg taaaacatcc
     8341 tccgcactta ccagtccgtt tcgcaaaaga taatccatcg ccctatcagg taatttgcaa
     8401 tcaggttttg cttttttcag ttggctgacc aattgtttaa ccagcattgt taattcgatc
     8461 acctggtagc gcggcaatgg tgaattatcg gatttgccct gactgtcatc actgcatgaa
     8521 tgcccttcca gccaggccaa tgcttgtcgc atgaaatacg caatatgttt gccgtggtaa
     8581 tcgtcttcat cgatgtgaaa agcgatactg cggatgtatt ctattgcatt ttcaatggcc
     8641 tccaacgcta tcggcgctgg cggtgcggta taaaacttcg tccccagcgg caacaacttc
     8701 atcgcttttt ctcccttaat gatgcgataa gttgatttcc cgccaaggtc taccgttcca
     8761 tccataacaa ggccgagtcg cttctctgaa acctcaccaa taggctctgc ttccagcgat
     8821 gccagagcaa ttcgtgccag ttcttccgct tcttctgctg gcagtacaac gttgctacca
     8881 ggtccgtatg tttcgcgcca ctgcttgatt gtcagtagtc gctctttggt aatagtggtc
     8941 atgggttatc ctcttagcgc cacaacgcgc acttctgggt caaaagatgg tgcattctca
     9001 tctgtaacat cagcaacatg acctacaaag tcgttatact caccctctga ttggtcatag
     9061 ccctgccaga ccacgacagc ctcaggaggc atttttctga gcttgctgat taactgccta
     9121 accgtcagcg acatctcact ctcctttgat gcgaatgcca gtagcgcgga tagatttcca
     9181 ttgtttccac attcggttga agtctgtgcc tgataaggtt gcgttacggt atccagtttc
     9241 ggtacgcaat ccttcaagcg tcgatttgga gatacaaatt gaatgcatta cccatgattc
     9301 aaacaaatca cgctcccggg cttcgcaact ttgcttactc atctcttcga tacgttcagc
     9361 catcgcagca cactcttcaa agttgcttaa tgcttttcgc tcccattcgg cgcattgttt
     9421 tccaagctct gcaatcagct tgtctttgcc ttccagctca acacgcagtt tccctaccgt
     9481 tagcgcaata tcctcgttct cctgatcgcg gcgtttgatg tattgctggt ttctttccag
     9541 ttcatccagt aatgccagca cagtagccgg attggctgcg gcgatgaatt cagcattggc
     9601 ctgctgttcc atttggaaat cttcatcgaa accgctttct ggatgtgctc cttcaattct
     9661 gcaaatggga agatatccaa caacttcacg atgaattaac gcatcatcac aatcaaatcg
     9721 gctctctcca tattcgagcg accatacacc acacgttgct ttttctgcct tggcacgcag
     9781 tgcctgatag tcaatcaggc tcactggttg cctcctttgc gaatctgttc cgcccattct
     9841 tcaagggatt tctccgcata ttcaccagac aggccatcaa tcggatgcgg ttcattagcc
     9901 aactcttctt tcgctgacag aatcatgcgc gtaacatcga aaacttcacg taaagactta
     9961 ttgataaatc cgtggttgaa agcagcagca agacggcttg cggcatagtt aataccctcg
    10021 ttgcgtgctt ccgcacgtac ttcagccagg aaagcatcgg tggctggggt tttgatttcg
    10081 ttaagcgcat cactgaataa accacgctcc atacctagct ctgcttcgta atcggcatcg
    10141 aatgcagcgt ctttgcagaa cttcttcatc cccgcactct ccgctgccag cgccgcgcac
    10201 ttggcctcaa ggttatcaat cgtgattcca gcagaacgac actcccgcaa cgccgtttcc
    10261 agttttgatt caagttcacc gaacttacgg acaagatatt cagcgtttgt ttcgttaacc
    10321 tttagatctc gtgggatgca tttacctttc agaaaaccat ccatctcaat tagtgacatt
    10381 tgtttcattt tttcccactc cgccacatag cattcagata tttgttgtca ttaacagaac
    10441 cgaaactctt tctcttaagc aattactctc tcgatggcat tggcttt
//
LOCUS       DAZKHF020000159        10321 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2201S31241
            SAMN43267800-rid25106413.denovo.160, whole genome shotgun sequence.
ACCESSION   DAZKHF020000159 DAZKHF020000000
VERSION     DAZKHF020000159.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267800
            Sequence Read Archive: SRR30307263
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 10321)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10321)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 10321)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350581.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350581.2
            Genome Coverage       :: 151x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:21:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,652
            CDSs (total)                      :: 5,557
            Genes (coding)                    :: 5,320
            CDSs (with protein)               :: 5,320
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 237
            CDSs (without protein)            :: 237
            Pseudo Genes (ambiguous residues) :: 0 of 237
            Pseudo Genes (frameshifted)       :: 102 of 237
            Pseudo Genes (incomplete)         :: 130 of 237
            Pseudo Genes (internal stop)      :: 52 of 237
            Pseudo Genes (multiple problems)  :: 41 of 237
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10321
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267800-rid25106413.denovo.160"
                     /serotype="O 26:H11"
                     /isolate="2201S31241"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-01"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            complement(228..2801)
                     /gene="clpB"
                     /locus_tag="ACHOSC_RS16240"
                     /old_locus_tag="pgaptmp_003248"
     CDS             complement(228..2801)
                     /gene="clpB"
                     /locus_tag="ACHOSC_RS16240"
                     /old_locus_tag="pgaptmp_003248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002461515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0009408 - response to heat [Evidence IEA];
                     GO_process: GO:0042026 - protein refolding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent chaperone ClpB"
                     /protein_id="HGU4549141.1"
                     /translation="MRLDRLTNKFQLALADAQSLALGHDNQFIEPLHLMSALLNQEGG
                     SVSPLLTSAGINAGQLRTDINQALNRLPQVEGTGGDVQPSQDLVRVLNLCDKLAQKRG
                     DNFISSELFVLAALESRGTLADILKAAGATTANITQAIEQMRGGESVNDQGAEDQRQA
                     LKKYTIDLTERAEQGKLDPVIGRDEEIRRTIQVLQRRTKNNPVLIGEPGVGKTAIVEG
                     LAQRIINGEVPEGLKGRRVLALDMGALVAGAKYRGEFEERLKGVLNDLAKQEGNVILF
                     IDELHTMVGAGKADGAMDAGNMLKPALARGELHCVGATTLDEYRQYIEKDAALERRFQ
                     KVFVAEPSVEDTIAILRGLKERYELHHHVQITDPAIVAAATLSHRYIADRQLPDKAID
                     LIDEAASSIRMQIDSKPEELDRLDRRIIQLKLEQQALMKESDEASKKRLDMLNEELSD
                     KERQYSELEEEWKAEKASLSGTQTIKAELEQAKIAIEQARRVGDLARMSELQYGKIPE
                     LEKQLEAATQLEGKTMRLLRNKVTDAEIAEVLARWTGIPVSRMMESEREKLLRMEQEL
                     HHRVIGQNEAVDAVSNAIRRSRAGLADPNRPIGSFLFLGPTGVGKTELCKALANFMFD
                     SDEAMVRIDMSEFMEKHSVSRLVGAPPGYVGYEEGGYLTEAVRRRPYSVILLDEVEKA
                     HPDVFNILLQVLDDGRLTDGQGRTVDFRNTVVIMTSNLGSDLIQERFGELDYAHMKEL
                     VLGVVSHNFRPEFINRIDEVVVFHPLGEQHIASIAQIQLKRLYKRLEERGYEIHISDE
                     ALKLLSENGYDPVYGARPLKRAIQQQIENPLAQQILSGELVPGKVIRLEVNEDRIVAV
                     Q"
     gene            complement(2931..3662)
                     /gene="yfiH"
                     /locus_tag="ACHOSC_RS16245"
                     /old_locus_tag="pgaptmp_003249"
     CDS             complement(2931..3662)
                     /gene="yfiH"
                     /locus_tag="ACHOSC_RS16245"
                     /old_locus_tag="pgaptmp_003249"
                     /EC_number="2.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004000 - adenosine deaminase activity
                     [Evidence IEA];
                     GO_function: GO:0004731 - purine-nucleoside phosphorylase
                     activity [Evidence IEA];
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="purine nucleoside phosphorylase YfiH"
                     /protein_id="HGU4549142.1"
                     /translation="MSKLIVPQWPLPKGVAACSSSRIGGVSLPPYDSLNLGAHCGDNP
                     DHVEENRKRLFAAGNLPSKPVWLEQVHGKDVLKLTGEPYASKRADASYSNTPGTVCAV
                     MTADCLPVLFCNRAGTEVAAAHAGWRGLCAGVLEETVSCFADNPENILAWLGPAIGPR
                     AFEVGVEVREAFMAVDAKASAAFIQHGDKYLADIYQLARQRLANVGVEQIFGGDRCTY
                     TENETFFSYRRDKTTGRMASFIWLI"
     gene            complement(3659..4639)
                     /gene="rluD"
                     /locus_tag="ACHOSC_RS16250"
                     /old_locus_tag="pgaptmp_003250"
     CDS             complement(3659..4639)
                     /gene="rluD"
                     /locus_tag="ACHOSC_RS16250"
                     /old_locus_tag="pgaptmp_003250"
                     /EC_number="5.4.99.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017383977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA pseudouridine(1911/1915/1917) synthase
                     RluD"
                     /protein_id="HGU4549143.1"
                     /translation="MAQRVQLTATVSENQLGQRLDQALAEMFPDYSRSRIKEWILDQR
                     VLVNGKVCDKPKEKVLGGELVAINAEIEEEARFEPQDIPLDIVYEDEDIIVINKPRDL
                     VVHPGAGNPDGTVLNALLHYYPPIADVPRAGIVHRLDKDTTGLMVVAKTVPAQTRLVE
                     SLQRREITREYEAVAIGHMTAGGTVDEPISRHPTKRTHMAVHPMGKPAVTHYRIMEHF
                     RVHTRLRLRLETGRTHQIRVHMAHITHPLVGDPVYGGRPRPPKGASEAFISMLRKFDR
                     QALHATMLRLYHPISGIEMEWHAPIPQDMVELIEVMRADFEEHKDEVDWL"
     gene            4774..5511
                     /gene="bamD"
                     /locus_tag="ACHOSC_RS16255"
                     /old_locus_tag="pgaptmp_003251"
     CDS             4774..5511
                     /gene="bamD"
                     /locus_tag="ACHOSC_RS16255"
                     /old_locus_tag="pgaptmp_003251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamD"
                     /protein_id="HGU4549144.1"
                     /translation="MTRMKYLVAAATLSLFLAGCSGSKEEVPDNPPNEIYATAQQKLQ
                     DGNWRQAITQLEALDNRYPFGPYSQQVQLDLIYAYYKNADLPLAQAAIDRFIRLNPTH
                     PNIDYVMYMRGLTNMALDDSALQGFFGVDRSDRDPQHARAAFSDFSKLVRGYPNSQYT
                     TDATKRLVFLKDRLAKYEYSVAEYYTERGAWVAVVNRVEGMLRDYPDTQATRDALPLM
                     ENAYRQMQMNAQAEKVAKIIAANSSNT"
     gene            5782..6123
                     /gene="raiA"
                     /locus_tag="ACHOSC_RS16260"
                     /old_locus_tag="pgaptmp_003252"
     CDS             5782..6123
                     /gene="raiA"
                     /locus_tag="ACHOSC_RS16260"
                     /old_locus_tag="pgaptmp_003252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated translation inhibitor RaiA"
                     /protein_id="HGU4549145.1"
                     /translation="MTMNITSKQMEITPAIRQHVADRLAKLEKWQTHLINPHIILSKE
                     PQGFVADATINTPNGVLVASGKHEDMYTAINELINKLERQLNKLQHKGEARRAATSVK
                     DANFVEEVEEE"
     misc_feature    6226..6351
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01859"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Phe leader region; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /db_xref="RFAM:RF01859"
     gene            6227..6274
                     /gene="pheL"
                     /locus_tag="ACHOSC_RS16265"
                     /old_locus_tag="pgaptmp_003253"
     CDS             6227..6274
                     /gene="pheL"
                     /locus_tag="ACHOSC_RS16265"
                     /old_locus_tag="pgaptmp_003253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0031556 - transcriptional attenuation by
                     ribosome [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pheA operon leader peptide PheL"
                     /protein_id="HGU4549146.1"
                     /translation="MKHTPFFFAFFFTFP"
     gene            6373..7533
                     /gene="pheA"
                     /locus_tag="ACHOSC_RS16270"
                     /old_locus_tag="pgaptmp_003254"
     CDS             6373..7533
                     /gene="pheA"
                     /locus_tag="ACHOSC_RS16270"
                     /old_locus_tag="pgaptmp_003254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004106 - chorismate mutase activity
                     [Evidence IEA];
                     GO_function: GO:0004664 - prephenate dehydratase activity
                     [Evidence IEA];
                     GO_process: GO:0009094 - L-phenylalanine biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0046417 - chorismate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional chorismate mutase/prephenate
                     dehydratase"
                     /protein_id="HGU4549147.1"
                     /translation="MTSENPLLALREKISALDEKLLALLAERRELAVEVGKAKLLSHR
                     PVRDIDRERDLLERLITLGKAHHLDAHYITRLFQLIIEDSVLTQQALLQQHLNKINPH
                     SARIAFLGPKGSYSHLAARQYAARHFEQFIESGCAKFADIFNQVETGQADYAVVPIEN
                     TSSGAINDVYDLLQHTSLSIVGEMTLTIDHCLLVSGTTDLSTINTVYSHPQPFQQCSK
                     FLNRYPHWKIEYTESTSAAMEKVAQAKSPHVAALGSEAGGTLYGLQVLERIEANQQQN
                     FTRFVVLARKAINVSDQVPAKTTLLMATGQQAGALVEALLVLRNHNLIMTRLESRPIH
                     GNPWEEMFYLDIQANLESAEMQKALKELGEITRSMKVLGCYPSENVVPVDPT"
     gene            complement(7576..8697)
                     /gene="tyrA"
                     /locus_tag="ACHOSC_RS16275"
                     /old_locus_tag="pgaptmp_003255"
     CDS             complement(7576..8697)
                     /gene="tyrA"
                     /locus_tag="ACHOSC_RS16275"
                     /old_locus_tag="pgaptmp_003255"
                     /EC_number="1.3.1.12"
                     /EC_number="5.4.99.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004106 - chorismate mutase activity
                     [Evidence IEA];
                     GO_function: GO:0004665 - prephenate dehydrogenase (NADP+)
                     activity [Evidence IEA];
                     GO_function: GO:0008977 - prephenate dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_process: GO:0006571 - tyrosine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0046417 - chorismate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional chorismate mutase/prephenate
                     dehydrogenase"
                     /protein_id="HGU4549148.1"
                     /translation="MVAELTALRDQIDEVDKALLNLLAKRLELVAEVGEVKSRFGLPI
                     YVPEREASMLASRRAEAEALGVPPDLIEDVLRRVMRESYSSENDKGFKTLCPSLRPVV
                     IVGGGGQMGRLFEKMLTLSGYQVRILEQHDWDRAADIVADAGMVIVSVPIHVTEQVIG
                     KLPPLPKDCILVDLASVKNGPLQAMLAAHDGPVLGLHPMFGPDSGSLAKQVVVWCDGR
                     KPEAYQWFLEQIQVWGARLHRISAVEHDQNMAFIQALRHFATFAYGLHLAEENVQLEQ
                     LLALSSPIYRLELAMVGRLFAQDPQLYADIIMSSERNLALIKRYYKRFGEAIELLEQG
                     DKQAFIDSFRKVEHWFGDYAQRFQSESRVLLRQANDNRQ"
     gene            complement(8708..9778)
                     /gene="aroF"
                     /locus_tag="ACHOSC_RS16280"
                     /old_locus_tag="pgaptmp_003256"
     CDS             complement(8708..9778)
                     /gene="aroF"
                     /locus_tag="ACHOSC_RS16280"
                     /old_locus_tag="pgaptmp_003256"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase AroF"
                     /protein_id="HGU4549149.1"
                     /translation="MQKDALNNVHITDEQVLMTPEQLKAAFPLSLQQEAQIADSRRTI
                     SDIIAGRDPRLLVVCGPCSIHDPETALEYARRFKALAAEVSDSLYLVMRVYFEKPRTT
                     VGWKGLINDPHMDGSFDVEAGLQIARKLLLELVNMGLPLATEALDPNSPQYLGDLFSW
                     SAIGARTTESQTHREMASGLSMPVGFKNGTDGSLATAINAMRAAAQPHRFVGINQAGQ
                     VALLQTQGNPDGHVILRGGKAPNYSPADVAQCEKEMEQAGLRPSLMVDCSHGNSNKDY
                     RRQPAVAESVVAQIKDGNRSIIGLMIESNIHEGNQSSEQPRSEMKYGVSVTDACISWE
                     MTDALLREIHQDLNGQLTARVA"
     gene            9988..>10321
                     /gene="yfiL"
                     /locus_tag="ACHOSC_RS16285"
                     /old_locus_tag="pgaptmp_003257"
     CDS             9988..>10321
                     /gene="yfiL"
                     /locus_tag="ACHOSC_RS16285"
                     /old_locus_tag="pgaptmp_003257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417093.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2799 domain-containing protein"
                     /protein_id="HGU4549150.1"
                     /translation="MMKKFIAPLLALLVSGCQIDPYTHAPTLTSTDWYDVGMEDAISG
                     SAIKDDDAFSDSQADRGLYLKGYAEGQKKTCQTDFTYARGLSGKSFPASCNNVENASQ
                     LHEVWQKGA"
BASE COUNT         2634 a         2732 c         2426 g         2529 t
ORIGIN      
        1 tttttgcttt ctctgccgga attctcagga gaaccccgct gacccggcgg cgtgtttgcc
       61 gttgttccgt gtcagtggtg gcgcattata gggagttatt ccggcctgac aagcgaaaaa
      121 tataaaaact ttatcgttcg ctcatttttc aggcaaagca tcttaaatat agtcttttcc
      181 gtctaactta tagacaaaaa cgagccccga agggctcgtt ttatcattta ctggacggcg
      241 acaatccggt cttcattaac ttccaggcga atcactttac ccggaaccaa ttcaccagac
      301 agtatttgct gtgccagcgg gttttcgatc tgctgctgga tagcacgttt caaaggacgt
      361 gcaccataga ctggatcgta accgttctcg ctcagcagtt tcagcgcctc gtcagaaatg
      421 tggatttcat aaccacgttc ttccagacgt ttgtacagac gtttcaactg aatctgcgca
      481 atcgaggcaa tgtgctgttc acccagcgga tggaagacca ccacttcatc aatacggtta
      541 atgaattccg gacggaagtt atggcttacc acaccgagca ccagctcttt catgtgcgca
      601 taatccagtt caccgaagcg ttcctgaatc aaatcggaac cgaggttaga ggtcataatg
      661 acaaccgtat tacggaagtc gaccgttctc ccctgcccgt cagtcagacg accatcatcc
      721 agtacctgca acagaatgtt gaagacatcc ggatgcgctt tttccacttc atccagcagg
      781 atgacggaat acggacgacg acgcacagct tcggtcaggt agccaccttc ttcataaccg
      841 acatatcccg gaggcgcacc aaccagacga gacaccgagt gtttctccat aaactcggac
      901 atatcgatac ggaccatcgc ctcgtcgcta tcaaacataa agttcgccag cgccttacaa
      961 agctcagttt tccccacacc cgttgggccg aggaacagga atgaaccaat cgggcgattt
     1021 ggatccgcca accccgcacg gctacggcga atagcgttag ataccgcatc aaccgcttcg
     1081 ttctgaccaa tgacgcgatg gtgcagttct tgctccatac gcagcagttt ttcgcgctcg
     1141 ctttccatca tgcgagaaac cggaatcccc gtccaacgcg ccagcacttc agcaatttcg
     1201 gcgtcggtca ctttattacg caacagacgc atagttttgc cttcgagctg cgttgcggct
     1261 tccagttgct tttccagttc cgggattttg ccgtattgca gttcagacat ccgcgccagg
     1321 tcccccacac ggcgagcctg ttcaatagcg attttcgcct gttccagttc cgctttaatg
     1381 gtctgcgtac cagaaagcga tgccttctct gctttccact cttcttctaa ctcggagtac
     1441 tgacgttctt tgtcgctcag ttcttcgttg agcatatcca ggcgtttttt actggcttca
     1501 tcagactctt tcattaacgc ctgttgttcc agtttgagct ggatgatacg acgatcgagt
     1561 cggtcgagtt cttctggttt tgagtcaatc tgcatacgaa tgctggatgc tgcttcatcg
     1621 atcaggtcga tggctttatc cggcagctga cggtcagcaa tgtagcgatg agacaacgtc
     1681 gccgctgcaa caattgccgg gtcagtaatt tgcacatggt ggtgcaattc gtaacgttct
     1741 ttcaggccac gcagaatcgc aatggtatct tcaacagaag gctcggcaac aaacactttc
     1801 tggaaacgac gttccagcgc agcatctttt tcgatgtact ggcgatattc gtcaagcgtc
     1861 gtggcaccta cgcagtgcaa ttcaccacgc gccagcgccg gtttcagcat gtttccggcg
     1921 tccattgcgc catcggcttt acccgcgccg accatggtat gtaattcgtc gataaacagg
     1981 atgacgttgc cttcctgttt ggcaagatcg ttaagcacgc cttttaaacg ttcttcaaac
     2041 tcaccgcgat atttcgcccc agccaccagc gcgcccatat ccagcgccag tacgcggcgg
     2101 cctttcaatc cttccggcac ttcgccgttg ataatacgct gcgccagacc ttcaacgatg
     2161 gcagttttac cgacgccggg ttcaccaatc agtaccgggt tatttttagt acgacgttgc
     2221 agcacctgaa tggtacggcg aatttcttca tcacgaccaa tcaccggatc gagtttgccc
     2281 tgttcggctc gttcggtaag gtcgatggta tattttttca aagcctgacg ttggtcttca
     2341 gcaccttgat cgttcacgct ttcacctcca cgcatttgtt caatcgcttg agtaatgttg
     2401 gcggtggtcg cccctgctgc tttcaggatg tcggccagcg tgccgcgaga ctcaagtgcc
     2461 gccagaacga acagttctga cgagataaag ttatcaccac gtttttgcgc cagcttgtcg
     2521 caaagattaa gaacgcgcac cagatcctgt gatggctgga catcaccacc agtaccttca
     2581 acctgcggta aacgatttaa tgcctgattg atatctgtgc gcaactggcc agcatttatg
     2641 ccagcggatg ttaataaagg actaaccgaa cccccttcct gattcagcag ggcgctcatt
     2701 aaatgaagtg gttcgataaa ttggttgtcg tgcccgagtg caagtgattg ggcatcggca
     2761 agagcaagct ggaatttatt agtaagacga tccagacgca taactcctcc cataacggat
     2821 caaagttgct actggagatt aaatgaggtc atccctcaat tattcaaggt tattgaccag
     2881 attaatgtga aaagaaaatc acgcgtaccg gatcgtcttg attctttagg ttatatcagc
     2941 caaatgaaac ttgccatacg accggtggtc ttgtcgcgac gataagagaa gaaagtctca
     3001 ttttccgtat atgtacaacg gtcgccgccg aaaatttgct caacacccac gttcgccaga
     3061 cgctgccggg caagctgata aatatccgcc agatacttat caccatgctg aatgaaagct
     3121 gcacttgctt tagcgtctac tgccataaac gcctcgcgaa cctccacccc cacttcgaac
     3181 gcgcgtggac caattgccgg ccctaaccag gcgagaatat tttccggatt atcagcaaaa
     3241 caggaaaccg tctcttccag cacgcctgcg cacagtccac gccagccagc atgagcggcg
     3301 gcgacttccg ttcccgctcg attgcaaaac agcacaggga ggcagtcggc agtcatcact
     3361 gcgcaaaccg tgccgggcgt attgctataa gaggcatctg ctcgttttga ggcataaggt
     3421 tcgccagtaa gcttaagcac atctttgccg tgtacctgct caagccagac cggtttagaa
     3481 ggcaaattgc ccgcagcaaa aagtcgcttg cgattctcct caacgtgatc cgggttatcg
     3541 ccacaatggg caccgaggtt gagcgagtca tacgggggca agctcacgcc gccgatacga
     3601 gaagagctac aggccgcaac accttttggt agcggccact gcgggacaat cagcttactc
     3661 ataaccagtc cacttcatcc ttatgctctt cgaaatcggc gcgcatcacc tcaatcagct
     3721 ccaccatatc ttgtggaata ggcgcatgcc attccatttc gatgccggag atcgggtgat
     3781 aaagacgcag catggttgca tgaagcgcct ggcggtcaaa cttacgcagc atggagataa
     3841 atgcttccga agcacctttt ggcggacgcg gacggccacc ataaaccgga tcgcccacca
     3901 gcggatgagt gatatgggcc atatgcacgc ggatctggtg cgtacgtcca gtttccagac
     3961 gcaaccgcag acgcgtgtgc acacggaagt gttccatgat gcgatagtga gtcaccgccg
     4021 gtttgcccat cggatgcacc gccatgtgag tacgtttggt cgggtggcgg ctgatgggtt
     4081 catctactgt accgccagcg gtcatatgac caatcgccac cgcttcatac tcacgagtaa
     4141 tttcacgccg ttgcaaagat tcgactaaac gcgtctgagc cggaacggtt tttgccacaa
     4201 ccatcaggcc agtggtgtct ttatccagac gatggacgat gcccgcacgt ggtacatcgg
     4261 caatgggcgg atagtaatga agcaacgcat tcagtaccgt gccatccggg ttacccgcgc
     4321 caggatgtac caccaggtcg cgcggtttat taatgacgat gatatcttca tcttcataga
     4381 cgatatccag cgggatatcc tgcggttcaa aacgcgcttc ttcttcaatc tcagcattga
     4441 tggcaaccag ctcgccaccc aatacttttt ctttcggctt atcacaaact ttgccgttta
     4501 ccagcacgcg ctggtcgagg atccattctt ttattcgcga acgtgaataa tccgggaaca
     4561 tttcggccaa agcctgatct aagcgttgac cgagttggtt ttcggacacc gttgcagtga
     4621 gctgtactcg ttgtgccata tatactgctt cttcgtttat cgttggggtt ttacggcttt
     4681 gccgtttaat atagtgtgct attgtagctg gtcttaaccg ggagcaggaa cagagaatct
     4741 cccgtattac attttgagga aagtcaaaac gtcatgacgc gcatgaaata tctggtggca
     4801 gccgccacac taagcctgtt tttggcgggt tgctcggggt caaaggaaga agtacctgat
     4861 aatccgccaa atgaaattta cgcgactgca caacaaaagc tgcaggacgg taactggaga
     4921 caggcaataa cgcaactgga agcgttagat aatcgctatc cgtttggtcc gtattcgcag
     4981 caggtgcagc tggatctcat ctacgcctac tataaaaacg ccgatttgcc gttagcgcag
     5041 gctgccatcg atcgttttat tcgccttaac ccgacccatc cgaatatcga ttatgtcatg
     5101 tacatgcgtg gcctgaccaa tatggcgctg gatgacagtg cgctgcaagg gttctttggc
     5161 gttgaccgta gcgatcgcga tcctcaacat gcacgagctg cgtttagtga cttttccaaa
     5221 ctggtgcgcg gctatccaaa cagtcagtac accaccgatg ccaccaaacg tctggtattc
     5281 ctgaaagatc gtctggcgaa atatgaatac tccgtggccg agtactatac agaacgtggc
     5341 gcatgggttg ccgtcgttaa ccgcgtagaa ggaatgttgc gcgactaccc ggatacccag
     5401 gctacgcgtg atgcgctgcc gctgatggaa aatgcatacc gtcagatgca gatgaatgcg
     5461 caagctgaaa aagtagcgaa aatcatcgcc gcaaacagca gcaatacata acagaaacct
     5521 gaaatacaaa acggcagccc ttgagctgcc gtttttttat tctgttagtt gtgaaactga
     5581 agcgatttag tcactatcga tctcatcaaa tatggctcgc tttgagatat tcctcaagta
     5641 aaaaaacacc tcttcctgcg atttctcaca aaaaagattc gttgacaaaa agtgacaaaa
     5701 ttatgagatt cccatcacac attttgacat caggagcggt atgctgaatt caccaagacg
     5761 ggaagacaag aggtaaaatt tatgacaatg aacattacca gcaaacaaat ggaaattact
     5821 ccggccatcc gccaacatgt cgcagaccgt ctcgccaaac tggaaaaatg gcaaacacat
     5881 ctgattaatc cacatatcat tctgtccaaa gagccacaag ggtttgttgc tgacgccaca
     5941 atcaatacac ctaacggcgt tctggttgcc agtggtaaac atgaagatat gtacactgca
     6001 attaacgaat tgatcaacaa gctggaacgg cagctcaata aactgcagca caaaggcgaa
     6061 gcacgtcgtg ccgcaacatc ggtgaaagac gccaacttcg tcgaagaagt tgaagaagag
     6121 tagtccttta tattgagtgt atcgccaacg cgccttcggg cgcgtttttt gttgacagcg
     6181 tgaaaacagt acgggtactg tactaaagtc acttaaggaa acaaacatga aacacacacc
     6241 gtttttcttc gcattctttt ttaccttccc ctgaatggga ggcgtttcgt cgtgtgaaac
     6301 agaatgcgaa gacgaacaat aaggcctccc aaatcggggg gcctttttta ttgataacaa
     6361 aaaggcaaca ctatgacatc ggaaaacccg ttactggcgc tgcgagagaa aatcagcgcg
     6421 ctggatgaaa aattattagc attactggca gagcggcgcg aattggccgt cgaggtagga
     6481 aaagccaaac tgctctcgca tcgcccggta cgagatattg atcgtgaacg cgatttactg
     6541 gaaagattaa ttacgctcgg taaagcgcac catctggacg cccattacat tactcgcctg
     6601 ttccagctca tcattgaaga ttccgtatta actcagcagg ctttgctcca gcaacatctc
     6661 aataaaatta atccgcactc agcacgcatc gcttttctcg gccccaaagg ttcttattcc
     6721 catcttgcgg cgcgccagta tgctgcccgt cactttgagc aattcattga aagtggctgc
     6781 gccaaatttg ccgatatttt taatcaggtg gaaaccggcc aggccgacta tgccgtcgta
     6841 ccgattgaaa ataccagctc cggtgccata aacgacgttt acgatctgct gcaacatacc
     6901 agcttgtcga ttgttggcga gatgacgtta actatcgacc attgtttgtt ggtctccggc
     6961 acgactgatt tatccactat taatacggtc tacagccatc cgcagccatt ccagcaatgc
     7021 agcaaattcc ttaatcgtta tcctcactgg aagattgaat ataccgaaag tacgtctgcg
     7081 gcaatggaaa aggttgcaca ggcaaaatca ccgcatgttg ctgcgttggg aagcgaagct
     7141 ggcggcactt tgtacggttt gcaggtactg gagcgtattg aagcgaatca gcaacaaaac
     7201 ttcacccgat ttgtggtgtt ggcacgtaaa gccattaacg tgtctgacca ggttccggcg
     7261 aaaacgacgt tgttaatggc gaccgggcaa caagccggtg cgctggttga agcgttgctg
     7321 gtgctgcgca accacaatct gattatgacc cgtctggaat cacgcccgat tcacggtaat
     7381 ccatgggaag agatgtttta tctggatatt caggccaatc ttgaatcagc ggaaatgcaa
     7441 aaagcattga aagagttagg ggaaattacc cgttcaatga aggtattggg ctgttaccca
     7501 agtgagaacg tagtgcctgt tgatccaacc tgatgaaaag gtgccggatg atgtgaatca
     7561 tccggcactg gattattact ggcggttgtc attcgcctga cgcaataaca cgcggctttc
     7621 actctgaaaa cgctgtgcgt aatcgccgaa ccagtgctcc accttgcgga aactgtcaat
     7681 aaacgcctgc ttatcgccct gctccagcaa ctcaatcgcc tcgccgaaac gcttatagta
     7741 acgtttgatt aacgccagat tacgctctga cgacataata atgtcggcat aaagctgcgg
     7801 atcctgagca aacagtcgcc cgaccatcgc cagctcaagg cggtaaatcg gcgaagagag
     7861 cgccagaagt tgctcaagct gaacattttc ttctgccaga tgcagcccat aagcaaaagt
     7921 agcaaagtgg cgcagagcct gaataaacgc catattctga tcgtgctcga cagcgctaat
     7981 acgatgcagc cgagcgcccc agacctgaat ttgctccaga aaccattggt atgcttccgg
     8041 cttacgtcca tcacaccaga ccacaacttg ctttgccagg ctaccgctgt ccgggccgaa
     8101 catcgggtgt aaccccagta ccgggccatc gtgcgccgcc agcatggcct gtaatggtcc
     8161 atttttcact gatgccagat caaccagaat acaatctttc ggtaaaggcg gtaatttgcc
     8221 gataacttgc tcagtaacgt ggattggcac actaacaatc accattccgg catcggcaac
     8281 aatatcagcc gctcgatccc agtcatgttg ctccagaatc cgcacctgat aacccgatag
     8341 tgtcagcatc ttctcgaaca ggcgtcccat ctgaccgcca ccgccgacga taaccaccgg
     8401 acgcagtgac ggacaaagtg ttttaaatcc tttgtcgttt tcactggagt aagattcacg
     8461 catcacccga cgcaaaacat cctcaatcag atctggcggt acacccagag cttccgcctc
     8521 tgcacgacgc gaggccaaca tagatgcctc gcgctccgga acataaatag gcagtccaaa
     8581 gcggcttttc acctcgccca cttcagcaac cagttccaga cgcttcgcta ataaattcag
     8641 cagcgcttta tcgacttcat caatttgatc gcgtaatgcg gtcaattcag caaccataat
     8701 aaacctctta agccacgcga gccgtcagct gcccgttcag atcctgatga atttcacgca
     8761 gcaaggcatc ggtcatttcc cagctaatgc aggcatcggt tacggataca ccgtatttca
     8821 tttcactgcg cggttgctca gaagactgat tgccctcgtg gatattactt tcgatcatca
     8881 gaccaataat tgagcgattg ccatctttga tttgagcaac cacggattct gccaccgcag
     8941 gctgacggcg ataatcttta ttggaattac cgtggctgca atctaccatc agagacgggc
     9001 gcagtcccgc ctgttccatc tctttttcac attgcgcaac atccgcaggg ctatagttcg
     9061 gcgctttacc accgcgcagg atgacatgcc cgtccggatt cccctgagtt tgtagcaacg
     9121 caacctgccc tgcctggtta atgccaacaa aacggtgcgg ctgggcggcg gcgcgcatag
     9181 cgttaattgc tgttgccaga ctgccgtcgg tgccgttttt aaaaccaacc ggcatggaaa
     9241 gcccggaggc catttcacgg tgagtttgcg attccgttgt acgagcacca attgctgacc
     9301 agctaaacag atcgcccagg tattgcgggc tattcggatc taacgcttcc gtcgccagtg
     9361 gcagtcccat attcaccagc tcaagcagca atttacgcgc gatctgcagc ccggcttcta
     9421 catcaaaaga gccatccata tggggatcgt taattaaccc tttccagccg acagtggtac
     9481 ggggtttttc aaaatagacg cgcattacca gatagaggct atcgctgacc tctgcggcaa
     9541 gggctttaaa tcgacgagca tattccagag cagtttccgg atcatgaatg gaacaaggac
     9601 cacatactac cagcagacga ggatcgcgcc cggcgataat atctgaaatg gttctacgcg
     9661 agtcagcaat ctgggcttct tgttgcaggc tcaatggaaa agcggccttc agttgttccg
     9721 gagtcattaa aacctgttcg tcggtaatat gtacgttatt cagcgcgtct ttttgcatga
     9781 tggcgatcct gtttatgctc gtttgcgata gttgatcctc agcgaggatg acgtaacgat
     9841 aacacataaa gtaaagtttt caatccatat ttcgtacatt tttatttaca caggcaattt
     9901 agtcgcgatt tcaaccctta cttctgtata gataaattta cactcccttt gaaaacaatc
     9961 cgctatgctt tgaaaaagga gaaagaaatg atgaaaaagt ttatcgcccc cttgttggct
    10021 ttactggtta gcggatgtca gattgatcct tatactcacg cgccaacctt gaccagcacc
    10081 gactggtatg atgttggtat ggaagatgcg atatcgggca gcgccataaa agatgacgat
    10141 gcatttagcg attcacaggc ggatcgcggt ctatacctta aaggatatgc cgaaggacaa
    10201 aagaaaactt gccagaccga ttttacttat gcccggggac tttccggtaa aagctttcct
    10261 gccagctgca acaatgttga aaatgccagc caactgcatg aagtatggca aaaaggggct
    10321 g
//
LOCUS       DAZKHF020000160        10285 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2201S31241
            SAMN43267800-rid25106413.denovo.161, whole genome shotgun sequence.
ACCESSION   DAZKHF020000160 DAZKHF020000000
VERSION     DAZKHF020000160.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267800
            Sequence Read Archive: SRR30307263
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 10285)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10285)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 10285)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350581.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350581.2
            Genome Coverage       :: 151x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:21:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,652
            CDSs (total)                      :: 5,557
            Genes (coding)                    :: 5,320
            CDSs (with protein)               :: 5,320
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 237
            CDSs (without protein)            :: 237
            Pseudo Genes (ambiguous residues) :: 0 of 237
            Pseudo Genes (frameshifted)       :: 102 of 237
            Pseudo Genes (incomplete)         :: 130 of 237
            Pseudo Genes (internal stop)      :: 52 of 237
            Pseudo Genes (multiple problems)  :: 41 of 237
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10285
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267800-rid25106413.denovo.161"
                     /serotype="O 26:H11"
                     /isolate="2201S31241"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-01"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            21..596
                     /gene="ssuE"
                     /locus_tag="ACHOSC_RS16290"
                     /old_locus_tag="pgaptmp_003258"
     CDS             21..596
                     /gene="ssuE"
                     /locus_tag="ACHOSC_RS16290"
                     /old_locus_tag="pgaptmp_003258"
                     /EC_number="1.5.1.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008752 - FMN reductase (NAD(P)H) activity
                     [Evidence IEA];
                     GO_process: GO:0046306 - alkanesulfonate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent FMN reductase"
                     /protein_id="HGU4549151.1"
                     /translation="MRVITLAGSPRFPSRSSSLLEYAREKLNCLDVEVYHWNLQNFAP
                     EDLLYARFDSPALKTFTEQLQQADGLIVATPVYKAAYSGALKTLLDLLPERALQGKVV
                     LPLATGGTVAHLLAVDYALKPVLSAMKAQEILHGVFADDSQVIDYHHKPQFTPNLQTR
                     LDTALETFWQALHRRDVQVPDLLSLRGNVHA"
     gene            589..1548
                     /gene="ssuA"
                     /locus_tag="ACHOSC_RS16295"
                     /old_locus_tag="pgaptmp_003259"
     CDS             589..1548
                     /gene="ssuA"
                     /locus_tag="ACHOSC_RS16295"
                     /old_locus_tag="pgaptmp_003259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415456.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aliphatic sulfonate ABC transporter
                     substrate-binding protein SsuA"
                     /protein_id="HGU4549152.1"
                     /translation="MRNIIKLALAGLLSVSTFAVAAESSPEALRIGYQKGSIGMVLAK
                     SHQLLEKRYPQSKISWVEFPAGPQMLEALNVGSIDLGSTGDIPPIFAQAAGADLVYVG
                     VEPPKPKAEVILVAENSPIKTVADLKGHKVAFQKGSSSHNLLLRALRQAGLKFTDIQP
                     TYLTPADARAAFQQGNVDAWAIWDPYYSAALLQGAVRVLKDGTDLNQTGSFYLAARPY
                     AEKNGAFIQGVLATFSEADALTRSQREQSIALLAKTMGLPAPVIASYLDHRPPTTIKP
                     VNAKVAALQQQTADLFYENRLVPKKVDIRQRIWQPTQLEGKQL"
     gene            1545..2690
                     /gene="ssuD"
                     /locus_tag="ACHOSC_RS16300"
                     /old_locus_tag="pgaptmp_003260"
     CDS             1545..2690
                     /gene="ssuD"
                     /locus_tag="ACHOSC_RS16300"
                     /old_locus_tag="pgaptmp_003260"
                     /EC_number="1.14.14.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008726 - alkanesulfonate monooxygenase
                     activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_process: GO:0006790 - sulfur compound metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FMNH2-dependent alkanesulfonate monooxygenase"
                     /protein_id="HGU4549153.1"
                     /translation="MSLNMFWFLPTHGDGHYLGTEEGSRPVDHGYLQQIAQAADRLGY
                     TGVLIPTGRSCEDAWLVAASMIPVTQRLKFLVALRPSVTSPTVAARQAATLDRLSNGR
                     ALFNLVTGSDPQELAGDGVFLDHSERYEASAEFTQVWRRLLLGETVNFNGKHIHVRGA
                     KLLFPPIQQPYPPLYFGGSSDVAQELAAEQVDLYLTWGEPPELVKEKIEQVRAKAAAH
                     GRKIRFGIRLHVIVRETNDEAWQAAERLISHLDDETIAKAQAAFARTDSVGQQRMAAL
                     HNGKRDNLEISPNLWAGVGLVRGGAGTALVGDGPTVAARINEYAALGIDSFVLSGYPH
                     LEEAYRVGELLFPHLDVAIPEIPQPQPLNPQGEAVANDFIPRKVAQS"
     gene            2701..3492
                     /gene="ssuC"
                     /locus_tag="ACHOSC_RS16305"
                     /old_locus_tag="pgaptmp_003261"
     CDS             2701..3492
                     /gene="ssuC"
                     /locus_tag="ACHOSC_RS16305"
                     /old_locus_tag="pgaptmp_003261"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415454.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aliphatic sulfonate ABC transporter permease
                     SsuC"
                     /protein_id="HGU4549154.1"
                     /translation="MATPVKKWLLRVAPWFLPVGIVAVWQLASSVGWLSTRILPSPEG
                     VVTAFWTLSASGELWQHLAISSWRALIGFSIGGSLGLILGLISGLSRWGERLLDTSIQ
                     MLRNVPHLALIPLVILWFGIDESAKIFLVALGTLFPIYINTWHGIRNIDRGLVEMARS
                     YGLSGIPLFIHVILPGALPSIMVGVRFALGLMWLTLIVAETISANSGIGYLAMNAREF
                     LQTDVVVVAIILYALLGKLADVSAQLLERLWLRWNPAYHLKEATV"
     gene            3489..4256
                     /gene="ssuB"
                     /locus_tag="ACHOSC_RS16310"
                     /old_locus_tag="pgaptmp_003262"
     CDS             3489..4256
                     /gene="ssuB"
                     /locus_tag="ACHOSC_RS16310"
                     /old_locus_tag="pgaptmp_003262"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aliphatic sulfonates ABC transporter ATP-binding
                     protein"
                     /protein_id="HGU4549155.1"
                     /translation="MNTARLNQGTPLLLNAVSKHYAENIVLNQLDLHIPAGQFVAVVG
                     RSGGGKSTLLRLLAGLETPTAGDVLAGTTPLAEIQEDTRMMFQDARLLPWKSVIDNVG
                     LGLKGQWRDAARRALAAVGLENRAGEWPAALSGGQKQRVALARALIHRPGLLLLDEPL
                     GALDALTRLEMQDLIVSLWQQHGFTVLLVTHDVSEAVAMADRVLLIEEGKIGLDLTVD
                     IPRPRRLGSVRLAELEAEVLQRVMRRGHSEQLIRRHG"
     gene            complement(4463..7075)
                     /gene="pepN"
                     /locus_tag="ACHOSC_RS16315"
                     /old_locus_tag="pgaptmp_003263"
     CDS             complement(4463..7075)
                     /gene="pepN"
                     /locus_tag="ACHOSC_RS16315"
                     /old_locus_tag="pgaptmp_003263"
                     /EC_number="3.4.11.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000193787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004177 - aminopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase N"
                     /protein_id="HGU4549156.1"
                     /translation="MTQQPQAKYRHDYRAPDYQITDIDLTFDLDAQKTVVTAVSQAVR
                     HGASDAPLRLNGEDLKLVSVHINDEPWTAWKEEEGALVISNLPERFTLKIINEISPAA
                     NTALEGLYQSGDALCTQCEAEGFRHITYYLDRPDVLARFTTKIIADKTKYPFLLSNGN
                     RVAQGELENGRHWVQWQDPFPKPCYLFALVAGDFDVLRDTFTTRSGREVALELYVDRG
                     NLDRAPWAMTSLKNSMKWDEERFGLEYDLDIYMIVAVDFFNMGAMENKGLNIFNSKYV
                     LARTDTATDKDYLDIERVIGHEYFHNWTGNRVTCRDWFQLSLKEGLTVFRDQEFSSDL
                     GSRAVNRINNVRTMRGLQFAEDASPMAHPIRPDMVIEMNNFYTLTVYEKGAEVIRMIH
                     TLLGEENFQKGMQLYFERHDGSAATCDDFVQAMEDASNVDLSHFRRWYSQSGTPIVTV
                     KDDYNPETEQYTLTISQRTPATPDQAEKQPLHIPFAIELYDNEGKVIPLQKGGHPVNS
                     VLNVTQAEQTFVFDNVYFQPVPALLCEFSAPVKLEYKWSDQQLTFLMRHARNDFSRWD
                     AAQSLLATYIKLNVARHQQGQPLSLPVHVADAFRAVLLDEKIDPALAAEILTLPSVNE
                     MAELFDIIDPIAIAEVREALTRTLATELADELLAIYNANYQSEYRVEHEDIAKRTLRN
                     ACLRFLAFGETHLADVLVSKQYHEANNMTDALAALSAAVAAQLPCRDALMQEYDDKWH
                     QDGLVMDKWFILQATSPAANVLETVRGLLQHRSFTMSNPNRIRSLIGAFAGSNPAAFH
                     AEDGSGYQFLVEMLTDLNSRNPQVASRLIEPLIRLKRYDAKRQEKMRAALEQLKGLEN
                     LSGDLYEKITKALA"
     gene            7341..8543
                     /gene="pncB"
                     /locus_tag="ACHOSC_RS16320"
                     /old_locus_tag="pgaptmp_003264"
     CDS             7341..8543
                     /gene="pncB"
                     /locus_tag="ACHOSC_RS16320"
                     /old_locus_tag="pgaptmp_003264"
                     /EC_number="6.3.4.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706850.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004516 - nicotinate
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate phosphoribosyltransferase"
                     /protein_id="HGU4549157.1"
                     /translation="MTQFASPVLHSLLDTDAYKLHMQQAVFHHYYDVHVAAEFRCRGD
                     DLLGIYADAIREQIQAMQHLRLQDDEYQWLSALPFFKADYLNWLREFRFNPEQVTVSN
                     DNGKLDIRLSGPWREVILWEVPLLAVISEMVHRYRSPQADVAQALDTLESKLVDFSAL
                     TAGLDMSRFHLMDFGTRRRFSREVQETIVKRLQQESWFVGTSNYDLARRLSLTPMGTQ
                     AHEWFQAHQQISPDLANSQRAALAAWLEEYPDQLGIALTDCITMDAFLRDFGVEFASR
                     YQGLRHDSGDPVEWGEKAVAHYEKLGIDPQSKTLVFSDNLDLRKAVELYRHFSSRVQL
                     SFGIGTRLTCDIPQVKPLNIVIKLVECNGKPVAKLSDSPGKTICHDKAFVRALRKAFD
                     LPHIKKAS"
     gene            8712..10112
                     /gene="asnS"
                     /locus_tag="ACHOSC_RS16325"
                     /old_locus_tag="pgaptmp_003265"
     CDS             8712..10112
                     /gene="asnS"
                     /locus_tag="ACHOSC_RS16325"
                     /old_locus_tag="pgaptmp_003265"
                     /EC_number="6.1.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004816 - asparagine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006421 - asparaginyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine--tRNA ligase"
                     /protein_id="HGU4549158.1"
                     /translation="MSVVPVADVLQGRVAVDSEVTVRGWVRTRRDSKAGISFLAVYDG
                     SCFDPVQAVINNSLPNYNEDVLRLTTGCSVIVTGKVVASPGQGQQFEIQASKVEVAGW
                     VEDPDTYPMAAKRHSIEYLREVAHLRPRTNLIGAVARVRHTLAQALHRFFNEQGFFWV
                     STPLITASDTEGAGEMFRVSTLDLENLPRNDQGKVDFDKDFFGKESFLTVSGQLNGET
                     YACALSKIYTFGPTFRAENSNTSRHLAEFWMLEPEVAFANLNDIAGLAEAMLKYVFKA
                     VLEERADDMKFFAERVDKDAVSRLERFIEADFAQVDYTDAVTILENCGRKFENPVYWG
                     VDLSSEHERYLAEEHFKAPVVVKNYPKDIKAFYMRLNEDGKTVAAMDVLAPGIGEIIG
                     GSQREERLDVLDERMLEMGLNKEDYWWYRDLRRYGTVPHSGFGLGFERLIAYVTGVQN
                     VRDVIPFPRTPRNASF"
BASE COUNT         2308 a         2670 c         2781 g         2526 t
ORIGIN      
        1 attggttata aggagagagt atgcgtgtca tcaccctggc gggtagtcct cgctttcctt
       61 ctcgctccag ctccttgctg gaatatgcgc gggaaaaact aaattgcctg gatgtagagg
      121 tttatcactg gaatctgcaa aacttcgccc cggaagatct gctttacgct cgtttcgata
      181 gtccggcact caagaccttc accgaacagc tgcaacaggc tgatggactg atagtcgcta
      241 cacctgtgta taaagccgcc tattccggcg cgctgaaaac cctgctcgac ctgctgccag
      301 agcgcgcttt gcaaggcaaa gtggtgctac cgctggcgac gggcggtacg gtggctcatc
      361 tgctggcggt cgattatgcc cttaagccag ttttaagtgc gatgaaagcc caggagatcc
      421 tgcacggcgt gtttgccgat gactcacaag ttattgatta ccatcacaaa ccccagttca
      481 cgccaaatct gcaaacccgt cttgataccg cgctagaaac tttctggcag gcattgcacc
      541 gccgcgatgt tcaggttcct gaccttctgt ctctgcgagg taatgtccat gcgtaacatc
      601 attaaactgg cgctggcggg attgcttagc gtctctactt ttgcggttgc tgcagaatcc
      661 tcgcctgaag cgttacgtat aggctatcag aaaggcagta ttggtatggt attggcaaaa
      721 agccaccagt tactggaaaa acgctatccg caatcaaaaa tctcctgggt ggagttcccc
      781 gcgggtccgc aaatgttgga agcgttaaac gttggcagta ttgatctcgg cagtaccggg
      841 gatattccgc caatctttgc ccaggctgcc ggggctgatt tggtatatgt gggcgtcgag
      901 ccaccgaagc ccaaagccga agtgattctg gtggcagaaa acagcccgat caaaaccgta
      961 gccgatctta aaggtcacaa agttgccttt cagaaaggtt ccagttcaca caacctttta
     1021 ctgcgtgcac tgcgtcaggc cggacttaag tttaccgata tccaacccac ttacctgacg
     1081 cccgctgatg cccgcgccgc gttccagcaa ggtaacgttg acgcctgggc tatctgggat
     1141 ccctactact ctgctgcatt attacagggc gccgtgcggg tgctgaaaga cggcaccgat
     1201 ctcaatcaaa ctggatcgtt ttatctggca gctcgtccct atgcagaaaa aaacggcgct
     1261 tttattcagg gcgtactcgc aacctttagt gaggccgatg cgttaacccg cagccagcgc
     1321 gagcaaagca tcgctttact ggcaaaaacg atgggcttac cggcaccggt gattgcctct
     1381 tacttagatc atcgccctcc taccaccatc aaaccggtta acgccaaggt tgccgcctta
     1441 cagcagcaaa cggcagatct gttttatgaa aatcgtctgg tgccgaaaaa agtcgatatt
     1501 cgccagcgca tctggcagcc cactcaactg gaaggaaaac aattatgagt ctgaatatgt
     1561 tctggttttt accgacccat ggtgacgggc attatcttgg aacggaagaa ggttcacgcc
     1621 cggttgatca cggctatctg caacaaattg cgcaagcggc ggatcgtctg ggctataccg
     1681 gtgtgctgat cccaacgggg cgatcgtgtg aagatgcgtg gctggtggcg gcgtcgatga
     1741 tcccggtgac gcagcggctg aagtttcttg tcgccctgcg ccccagcgta acctcaccta
     1801 ccgttgccgc tcgccaggcc gccacgcttg accgtctctc aaatggtcgt gcgttgttta
     1861 atctggtcac aggcagcgat ccacaagagc tggcaggcga cggcgtgttc cttgatcata
     1921 gcgagcgcta cgaagcctcg gcggaattta cccaggtctg gcggcgctta ttgctggggg
     1981 aaaccgtcaa tttcaacggt aaacatattc atgtgcgcgg agcaaaactg ctcttcccgc
     2041 cgattcaaca gccttatcct ccgctttact ttggcggatc gtcagacgtt gcccaggagc
     2101 tggcggcaga acaagttgat ctctacctca cctggggcga accgccagaa ctagtaaaag
     2161 agaagatcga acaagtgcgg gcgaaagctg ccgcgcatgg acgcaaaatt cgtttcggta
     2221 ttcgtctgca tgtgattgtt cgtgaaacta acgacgaagc gtggcaggcc gccgagcggt
     2281 taatctcgca tcttgatgat gaaactatcg ccaaagcaca ggccgcattc gcccggacgg
     2341 attccgtagg acaacagcga atggcggcgt tacataacgg caagcgcgac aatctggaga
     2401 tcagccccaa tttatgggcg ggcgttggct tagtgcgcgg cggtgccgga acggcgctgg
     2461 tgggcgatgg tcctacggtc gctgcgcgaa tcaacgaata cgccgcgctt ggcatcgaca
     2521 gctttgtgct ttcgggctat ccgcatctgg aagaagcgta tcgggtcggc gagttgctgt
     2581 tcccacatct ggatgtcgcc attccggaaa ttccccagcc gcagccgctg aatccgcaag
     2641 gcgaagcggt ggcgaatgat tttatccccc gtaaagtcgc gcaaagctaa ggaagaaata
     2701 atggcaacgc cagtgaagaa gtggttattg cgcgttgccc cctggttttt accggtgggc
     2761 atcgtggcgg tgtggcaact ggcctcctca gttggctggc tgtcgacgcg tattttgccc
     2821 tcaccggaag gggtggtgac ggcgttctgg acgctctccg ccagcggcga actgtggcag
     2881 catctggcga tcagttcctg gcgggcgctg attggctttt caattggcgg atcgctgggg
     2941 ttgattttgg gactgattag cgggctatct cgctggggag aacggctgct ggatacctca
     3001 attcagatgt tgcgcaacgt accgcatctg gcgctgatcc cgctggtgat tttgtggttt
     3061 ggcattgatg aatccgcaaa aatctttctg gtggcgctcg gcacgctatt ccccatttat
     3121 atcaacacct ggcatggcat ccgtaatatc gatcgcggtc tggtggaaat ggcgcgtagc
     3181 tatggattat ccggcatacc gctgtttatc catgtgatcc tgcctggtgc cctgccctca
     3241 attatggtcg gcgtgcgttt tgcgttgggc ctgatgtggc tgacgctgat tgttgccgaa
     3301 accatttctg ccaattcagg cattggttat ctggcgatga atgcgcggga gtttttgcaa
     3361 acggacgtgg tggtagtcgc tattattctt tacgccctgc tcggcaaact tgctgacgtc
     3421 agcgcgcagc tgttagagcg cctctggttg cgctggaacc cggcttatca tttgaaggag
     3481 gccacggtat gaatactgct cgtctgaacc agggcacacc attgttgctc aatgcagtaa
     3541 gcaaacatta cgcggaaaat atcgtcctga accaactgga tttacatatt ccggcaggtc
     3601 agtttgtggc ggtggtgggc cgcagcggtg gtggcaaaag taccctgctg cgcctgctgg
     3661 caggtctgga aacgccaacc gcaggcgatg tgttagcggg caccacaccg ctggctgaga
     3721 ttcaagaaga cacgcgaatg atgtttcagg atgcgcgtct gctgccgtgg aaatcggtga
     3781 ttgataacgt tgggttaggc cttaaaggcc agtggcgcga tgccgcgcgt cgggcgttgg
     3841 ctgcagtagg actggagaat cgcgccgggg aatggcctgc cgcactttct ggcgggcaga
     3901 aacagcgtgt ggcgctggca agagcgttga ttcatcgacc gggtttattg ttgcttgatg
     3961 aaccgctcgg ggcgctggac gccttaacgc gactcgagat gcaggatttg attgtgtcac
     4021 tttggcagca gcacggcttt accgtactgt tggtgacgca tgatgtgagc gaagcggtag
     4081 caatggctga ccgggtgttg ttaattgaag agggaaaaat tggtctggat ttgacagtgg
     4141 atatcccccg tccgcgtcga ttagggtcgg tgcgattagc ggagctggaa gcggaagtat
     4201 tgcaacgggt aatgcggcgt gggcattctg aacaactgat tcggcgtcat ggttaatatg
     4261 ccggatgtgg cgcacaaaac cagcattttc aacggactag taggcctgat aagacacgtc
     4321 aagcatcgca tcaggcagtt gggcacggct gccggatgcg gcgtgaacgc cttatccagc
     4381 ctacaaaacc agcaatttca acggagttgt aggcctgata agacgcgtca agcgtcgcat
     4441 caggcatttt ggctcaaaag tatcaagcca gtgctttggt gatcttctcg tacagatcgc
     4501 cagagagatt ttccagccct ttcaactgtt ccagcgccgc gcgcattttc tcctgacgtt
     4561 tggcatcata acgtttcagg cgaatcagcg gttcaatcag acgtgaagct acctgcgggt
     4621 tacggctgtt gaggtcggta agcatttcca ccaggaactg gtaaccgctg ccatcttcgg
     4681 catggaacgc tgccgggttg ctgcccgcaa acgcaccaat caacgaacga atacggttcg
     4741 ggttgctcat ggtaaatgag cgatgctgca acagaccacg caccgtctcc agcacattcg
     4801 ccgccgggct ggtggcttgc aggataaacc atttatccat caccagaccg tcctgatgcc
     4861 acttatcgtc gtactcctgc atcagcgcgt cacggcaagg cagctgtgca gcaaccgccg
     4921 cagaaagcgc tgccagcgca tcggtcatat tgtttgcttc atggtattgc ttgctcacca
     4981 gcacgtcagc cagatgcgtt tcaccaaaag caaggaagcg caggcaggca ttacgcagag
     5041 tgcgttttgc aatatcttca tgctcaacac ggtactcgct ctggtaattc gcgttgtaaa
     5101 tagccagcag ctcatccgcc agttcagtcg ccagagtacg agtgagtgct tcgcgtactt
     5161 cggcaatagc aatcgggtcg atgatatcga acagttcagc catttcattg acagaaggca
     5221 gcgtcaggat ttctgccgcc agcgccggat caatcttctc atcgagcagc accgcgcgga
     5281 aagcgtcagc cacatgtacc ggcagagaca gcggctgccc ttgctgatga cgggcgacgt
     5341 tcagcttgat gtaggttgcc agcaaacttt gcgccgcatc ccaacgggag aaatcattac
     5401 gcgcatgacg catcaggaag gtcagttgct gatcgctcca cttatattcc agtttcactg
     5461 gcgcagagaa ttcgcacagc agcgcaggca ccggctggaa gtagacatta tcaaaaacaa
     5521 aggtctgttc cgcctgggtg acgttcagca cggaattcac cggatgaccg cctttctgca
     5581 acgggatcac tttgccttcg ttgtcataca attcgatggc aaatggaata tgcagcggct
     5641 gtttttctgc ctgatccggc gtggctggcg tgcgctggct gatggtcagg gtgtactgct
     5701 cggtttccgg attgtagtcg tctttgacgg tcacaatcgg cgtgccggac tggctgtacc
     5761 aacggcggaa atgggagaga tcgacattcg acgcatcttc catcgcctgc acaaagtcgt
     5821 cgcaggttgc cgcgctgcca tcatggcgct cgaaatagag ttgcatccct ttctggaagt
     5881 tttcttcgcc cagcagggtg tggatcatgc gaatcacttc cgcgcccttc tcgtaaacgg
     5941 tcagggtgta gaagttgttc atctcaatga ccatatccgg gcggatcggg tgcgccatcg
     6001 ggctggcgtc ttccgcaaac tgcaatccgc gcatggtgcg cacattattg atacggttta
     6061 ccgcgcggga accaaggtca gagctgaatt cctgatcgcg gaagacggtt aaaccttctt
     6121 tcaggctgag ctggaaccag tcgcggcagg tcactcggtt accggtccag ttatggaaat
     6181 attcatggcc gataacgcgt tcaatatcga gataatcttt gtcggtggcg gtgtcggtgc
     6241 gggccaacac atatttggag ttaaagatat tcagaccctt attctccatt gcgcccatat
     6301 tgaagaaatc caccgcgacg atcatataga tgtcgaggtc atactccagg ccaaagcgtt
     6361 cttcatccca tttcatggag tttttcagcg aggtcatcgc ccacggcgcg cgatcaaggt
     6421 tgccgcgatc gacgtacagc tccagtgcta cttcgcgacc agaacgcgtg gtaaaggtgt
     6481 cgcgcagtac atcaaagtcg cctgccacca gcgcaaacag gtagcacggt ttcgggaacg
     6541 ggtcctgcca ctgtacccaa tggcgtccgt tttccagttc accttgcgca acgcggttac
     6601 cgttggaaag caggaaggga tatttggttt tatcggcaat aattttggtg gtaaaacgcg
     6661 ccagcacgtc cgggcggtcg agataatacg taatatggcg gaaaccttcg gcttcacact
     6721 gggtgcaaag cgcatcgcct gactgataaa gcccttccag cgccgtattc gccgccgggc
     6781 ttatttcatt aatgatctta agcgtaaaac gctccggcaa attactgatg accagtgcgc
     6841 cctcttcttc tttccaggcg gtccacggct catcattaat atgaacagaa accagtttga
     6901 ggtcttcgcc gttgagacga agcggagcat ctgatgcacc atgacggaca gcctggctga
     6961 ccgcggtaac gaccgtcttt tgcgcgtcga ggtcaaaggt caagtcaata tcagtaatct
     7021 ggtaatccgg cgcacgataa tcgtgacggt atttggcttg tggctgttga gtcataaata
     7081 acctttagca tcttttatag agtctggtgt tcagtctatt cctgttgcgt aaatcgcgct
     7141 atgcagaatc ttcatctttt caggtacaaa cgcctttatt gctacatttt tataacatac
     7201 accgcgtaat gccatcgacc agaaaggtgg catatggtgt gatcggggtt caataaattg
     7261 cgaaacaagg tatactccag cagttcctga agatgtttat tgtactaaac gctcctgtac
     7321 gaggacgcta ctgcgcacct atgacacaat tcgcttctcc tgttctgcac tcgttgctgg
     7381 atacagatgc ttataagttg catatgcagc aagccgtgtt tcatcactat tacgatgtgc
     7441 atgtcgcggc ggagtttcgt tgccgaggtg acgatctgct gggtatttat gccgatgcta
     7501 ttcgtgaaca gattcaggcg atgcagcacc tgcgcctgca ggatgatgaa tatcagtggc
     7561 tctctgccct gcctttcttt aaggccgact atcttaactg gttacgcgag ttccgcttta
     7621 acccggaaca agtcaccgta tccaacgata atggcaagct ggatattcgt ttaagcggcc
     7681 cgtggcgtga agtcatcctc tgggaagttc ctttgctggc ggttatcagt gaaatggtac
     7741 atcgctatcg ctcaccgcag gccgacgttg cgcaagccct cgacacgctg gaaagcaaat
     7801 tagtcgactt ctcggcgtta accgccggcc ttgatatgtc gcgcttccat cttatggatt
     7861 ttggcacccg tcgccgtttt tctcgcgaag tacaagaaac catcgttaag cgtctgcaac
     7921 aggaatcctg gttcgtgggc accagcaact acgatctggc gcgtcggctt tccctcacgc
     7981 cgatgggaac acaggcacac gaatggttcc aggcacatca gcaaatcagc ccggatctag
     8041 ccaacagcca gcgagctgca cttgctgcct ggctggaaga atatcccgac caacttggca
     8101 ttgcattaac cgactgcatc actatggatg ctttcctgcg tgatttcggt gtcgagttcg
     8161 ccagtcgcta tcagggcctg cgtcatgact ctggcgaccc ggttgaatgg ggtgaaaaag
     8221 ccgttgcaca ttatgaaaag ctgggaattg atccacagag taaaactctg gttttctctg
     8281 acaatctgga tttacgcaaa gcggttgagc tataccgcca cttctcttcc cgcgtgcaat
     8341 taagttttgg tattgggact cgcctgacct gcgatatccc ccaggtaaaa cccctgaata
     8401 ttgtgattaa gttggttgag tgtaacggta aaccggtggc gaaactttct gacagccctg
     8461 gcaaaactat ctgccacgat aaagcgtttg ttcgggcgct gcgcaaagcg ttcgaccttc
     8521 cgcatattaa aaaagccagt taatatcatc agggagctaa tcggctccct ttttttacct
     8581 ttaattccga aatctttcgc tgcatttgcg aattctgctt gtctgattgc agatgccagg
     8641 taacataggt atccccccat ttacgggtgg acaagtgttt attttttccg actattaaca
     8701 gagagaatat tatgagcgtt gtgcctgtag ccgacgtact ccagggccgc gtagccgttg
     8761 acagcgaagt caccgtacgc ggatgggtac gtacccgccg agattcaaaa gctggcatct
     8821 ccttcctcgc cgtttatgac ggttcctgct ttgatcctgt acaggctgtc atcaataatt
     8881 ctctgcccaa ttacaatgaa gacgtcctgc gtctgaccac cggctgctcg gtcattgtga
     8941 cgggtaaagt cgtggcgtcg ccgggccagg ggcaacaatt tgaaattcag gccagcaagg
     9001 ttgaagttgc tggttgggtt gaagatccag acacttaccc gatggcggca aaacgccaca
     9061 gcattgagta tctgcgtgaa gtcgctcacc tgcgtccgcg cacaaacctg attggtgccg
     9121 tcgcgcgcgt tcgtcatacg ctggcgcagg cgctgcatcg cttctttaac gagcagggat
     9181 tcttctgggt ttcaacgcca ctgattaccg catctgatac cgaaggtgca ggcgaaatgt
     9241 tccgcgtttc tacgctggat ctggaaaacc tgccgcgtaa cgatcagggc aaagtggatt
     9301 tcgacaaaga cttctttggt aaagagtctt tcctgaccgt atctggccag ttgaacggcg
     9361 aaacctacgc ttgcgcatta tccaaaattt ataccttcgg cccgactttc cgtgctgaaa
     9421 actccaacac cagccgtcac ctggcggaat tctggatgct ggagccggaa gtggcgtttg
     9481 ctaacctgaa cgatatcgcg ggtctggctg aagccatgct gaaatatgtc ttcaaagcgg
     9541 ttctcgaaga acgcgcagac gacatgaaat tcttcgctga acgcgtagat aaagatgccg
     9601 tttcacgtct ggaacgcttc attgaggccg atttcgcgca ggtggattac accgacgcag
     9661 tgaccattct cgaaaactgc ggcaggaagt ttgaaaaccc ggtttactgg ggcgtcgatc
     9721 tctcttctga gcatgagcgt tatctggcgg aagaacactt taaagcaccg gtagtggtta
     9781 aaaactatcc gaaagatatt aaagcgttct atatgcgcct caacgaagac ggtaaaaccg
     9841 ttgcggctat ggacgttctg gctccgggca tcggtgagat cattggtggc tcccagcgtg
     9901 aagaacgtct ggacgttctg gacgagcgta tgctggaaat gggcctgaat aaagaagatt
     9961 actggtggta tcgcgatctg cgtcgctacg gtactgttcc gcattcaggt ttcggtcttg
    10021 gttttgaacg cctgattgct tacgtaactg gcgtgcaaaa cgtgcgtgat gtgattccgt
    10081 tcccacgtac tccgcgcaac gccagcttct gattttcttc ctcaaagagc cagcgcctgc
    10141 tggctctttt ttatcctttc tgtcaagtta tctgtttgtt aagtcaagca atctatttgc
    10201 aaccccgcca taacgatcat cctgttacgg aatattacat tgcaacattt acgtacaaaa
    10261 aataacccgc attcttattg cggat
//
LOCUS       DAZKHF020000161        10277 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2201S31241
            SAMN43267800-rid25106413.denovo.162, whole genome shotgun sequence.
ACCESSION   DAZKHF020000161 DAZKHF020000000
VERSION     DAZKHF020000161.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267800
            Sequence Read Archive: SRR30307263
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 10277)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10277)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 10277)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350581.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350581.2
            Genome Coverage       :: 151x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:21:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,652
            CDSs (total)                      :: 5,557
            Genes (coding)                    :: 5,320
            CDSs (with protein)               :: 5,320
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 237
            CDSs (without protein)            :: 237
            Pseudo Genes (ambiguous residues) :: 0 of 237
            Pseudo Genes (frameshifted)       :: 102 of 237
            Pseudo Genes (incomplete)         :: 130 of 237
            Pseudo Genes (internal stop)      :: 52 of 237
            Pseudo Genes (multiple problems)  :: 41 of 237
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10277
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267800-rid25106413.denovo.162"
                     /serotype="O 26:H11"
                     /isolate="2201S31241"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-01"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            161..1555
                     /gene="galP"
                     /locus_tag="ACHOSC_RS16330"
                     /old_locus_tag="pgaptmp_003266"
     CDS             161..1555
                     /gene="galP"
                     /locus_tag="ACHOSC_RS16330"
                     /old_locus_tag="pgaptmp_003266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose/proton symporter"
                     /protein_id="HGU4549159.1"
                     /translation="MPDAKKQGRSNKAMTFFVCFLAALAGLLFGLDIGVIAGALPFIA
                     DEFQITSHTQEWVVSSMMFGAAVGAVGSGWLSFKLGRKKSLMIGAILFVAGSLFSAAA
                     PNVEVLILSRVLLGLAVGVASYTAPLYLSEIAPEKIRGSMISMYQLMITIGILGAYLS
                     DTAFSYTGAWRWMLGVIIIPAILLLIGVFFLPDSPRWFAAKRRFVDAERVLLRLRDTS
                     AEAKRELDEIRESLQVKQSGWALFKENSNFRRAVFLGVLLQVMQQFTGMNVIMYYAPK
                     IFELAGYTNTTEQMWGTVIVGLTNVLATFIAIGLVDRWGRKPTLTLGFLVMAAGMGVL
                     GTMMHIGIHSPSAQYFAIAMLLMFIVGFAMSAGPLIWVLCSEIQPLKGRDFGITCSTA
                     TNWIANMIVGATFLTMLNTLGNANTFWVYAALNVLFILLTLWLVPETKHVSLEHIERN
                     LMKGRKLREIGAHD"
     gene            1632..2129
                     /locus_tag="ACHOSC_RS16335"
                     /old_locus_tag="pgaptmp_003267"
     CDS             1632..2129
                     /locus_tag="ACHOSC_RS16335"
                     /old_locus_tag="pgaptmp_003267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SprT family zinc-dependent metalloprotease"
                     /protein_id="HGU4549160.1"
                     /translation="MKTSRLPIAIQQAVMRRLREKLAQANLKLGRNYPEPKLSYTQRG
                     TSAGTAWLESYEIRLNPVLLLENSEAFIEEVVPHELAHLLVWKHFGRVAPHGKEWKWM
                     MESVLGVPARRTHQFELQSVRRNTFPYRCKCQEHQLTVRRHNRVVRGEAVYRCVHCGE
                     QLVAK"
     gene            2224..2931
                     /gene="endA"
                     /locus_tag="ACHOSC_RS16340"
                     /old_locus_tag="pgaptmp_003268"
     CDS             2224..2931
                     /gene="endA"
                     /locus_tag="ACHOSC_RS16340"
                     /old_locus_tag="pgaptmp_003268"
                     /EC_number="3.1.21.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004518 - nuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribonuclease I"
                     /protein_id="HGU4549161.1"
                     /translation="MYRYLSIAAVVLSAAFSGPALAEGINSFSQAKAAAVKVHADAPG
                     TFYCGCKINWQGKKGVVDLQSCGYQVRKNENRASRVEWEHVVPAWQFGHQRQCWQDGG
                     RKNCAKDPVYRKMESDMHNLQPSVGEVNGDRGNFMYSQWNGGEGQYGQCAMKVDFKEK
                     AAEPPARARGAIARTYFYMRDQYNLTLSRQQTQLFNAWNKMYPVTDWECERDERIAKV
                     QGNHNPYVQRACQARKS"
     gene            3011..3742
                     /gene="rsmE"
                     /locus_tag="ACHOSC_RS16345"
                     /old_locus_tag="pgaptmp_003269"
     CDS             3011..3742
                     /gene="rsmE"
                     /locus_tag="ACHOSC_RS16345"
                     /old_locus_tag="pgaptmp_003269"
                     /EC_number="2.1.1.193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311849.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (uracil(1498)-N(3))-methyltransferase"
                     /protein_id="HGU4549162.1"
                     /translation="MRIPRIYHPEPLTSHSHIALCEDAANHIGRVLRMGPGQALQLFD
                     GSNQVFDAEITSASKKSVEVKVLEGKIDDRESPLHIHLGQVMSRGEKMEFTIQKSIEL
                     GVSLITPLFSERCGVKLDSERLNKKLQQWQKIAIAACEQCGRNRVPEIRPAMDLEAWC
                     AEQDEGLKLNLHPRASNSINTLPLPVERVRLLIGPEGGLSADEIAMTARYQFTDILLG
                     PRVLRTETTALTAITALQVRFGDLG"
     gene            3755..4705
                     /gene="gshB"
                     /locus_tag="ACHOSC_RS16350"
                     /old_locus_tag="pgaptmp_003270"
     CDS             3755..4705
                     /gene="gshB"
                     /locus_tag="ACHOSC_RS16350"
                     /old_locus_tag="pgaptmp_003270"
                     /EC_number="6.3.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004363 - glutathione synthase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006750 - glutathione biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione synthase"
                     /protein_id="HGU4549163.1"
                     /translation="MIKLGIVMDPIANINIKKDSSFAMLLEAQRRGYELHYMEMGDLY
                     LINGEARAHTRTLNVKQNYEEWFSFVGEQDLPLADLDVILMRKDPPFDTEFIYATYIL
                     ERAEEKGTLIVNKPQSLRDCNEKLFTAWFSDLTPETLVTRNKAQLKAFWEKHSDIILK
                     PLDGMGGASIFRVKEGDPNLGVIAETLTEHGTRYCMAQNYLPAIKDGDKRVLVVDGEP
                     VPYCLARIPQGGETRGNLAAGGRGEPRPLTESDWKIARQIGPTLKEKGLIFVGLDIIG
                     DRLTEINVTSPTCIREIEAEFPVSITGMLMDAIEARLQQQ"
     gene            4814..5377
                     /locus_tag="ACHOSC_RS16355"
                     /old_locus_tag="pgaptmp_003271"
     CDS             4814..5377
                     /locus_tag="ACHOSC_RS16355"
                     /old_locus_tag="pgaptmp_003271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417423.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqgE/AlgH family protein"
                     /protein_id="HGU4549164.1"
                     /translation="MNLQHHFLIAMPALQDPIFRRSVVYICEHNTNGAMGIIVNKPLE
                     NLKIEGILEKLKITPEPRDESIRLDKPVMLGGPLAEDRGFILHTPPSNFASSIRISDN
                     TVMTTSRDVLETLGTDKQPSDVLVALGYASWEKGQLEQEILDNAWLTAPADLNILFKT
                     PIADRWREAAKLIGVDILTMPGVAGHA"
     gene            5377..5793
                     /gene="ruvX"
                     /locus_tag="ACHOSC_RS16360"
                     /old_locus_tag="pgaptmp_003272"
     CDS             5377..5793
                     /gene="ruvX"
                     /locus_tag="ACHOSC_RS16360"
                     /old_locus_tag="pgaptmp_003272"
                     /EC_number="3.1.21.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction resolvase RuvX"
                     /protein_id="HGU4549165.1"
                     /translation="MSGTLLAFDFGTKSIGVAVGQRITGTARPLPAIKAQDGTPDWNL
                     IERLLKEWQPDEIIVGLPLNMDGTEQPLTARARKFANRIHGRFGVEVKLHDERLSTVE
                     ARSGLFEQGGYRALNKGKVDSASAVIILESYFEQGY"
     gene            complement(5969..6949)
                     /locus_tag="ACHOSC_RS16365"
                     /old_locus_tag="pgaptmp_003273"
     CDS             complement(5969..6949)
                     /locus_tag="ACHOSC_RS16365"
                     /old_locus_tag="pgaptmp_003273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417425.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV pilus twitching motility protein PilT"
                     /protein_id="HGU4549166.1"
                     /translation="MNMEEIVALSVKHNVSDLHLCSAWSARWRIRGLMEAAPFDAPDV
                     EELLREWLDDDQRAILLENGQLDFAVSLAENQRLRGSAFAQRQGISLALRLLPSHCPQ
                     LEQLGAPTVLPELLKSENGLILVTGATGSGKSTTLAAMVGYLYQHADAHILTLEDPVE
                     YIYASQRCLIQQREIGLHCMTFASGLRAALREDPDVILLGELRDSETIRLALTAAETG
                     HLVLATLHTRGAAQAVERLVDSFPAQEKDPVRNQLAGSLRAVLSQKLEVDKQEGRVAL
                     FELLINTPAVGNLIREGKTHQLPHVIQTGQQVGMLTFQQSYQQRVGEGRL"
     gene            6967..7671
                     /gene="yggS"
                     /locus_tag="ACHOSC_RS16370"
                     /old_locus_tag="pgaptmp_003274"
     CDS             6967..7671
                     /gene="yggS"
                     /locus_tag="ACHOSC_RS16370"
                     /old_locus_tag="pgaptmp_003274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate homeostasis protein"
                     /protein_id="HGU4549167.1"
                     /translation="MNDIAHNLAQVRDKISAAATRCGRSPEEITLLAVSKTKPASAIA
                     EAIDAGQRQFGENYVQEGVDKIRHFQELGVTGLEWHFIGPLQSNKSRLVAEHFDWCHT
                     IDRLRIATRLNDQRPAELPPLNVLIQINISDENSKSGIQLAELDELAAAVAELPRLRL
                     RGLMAIPAPESEYVRQFEVARQMAVAFAGLKTRYPHIDTLSLGMSDDMEAAIAAGSTM
                     VRIGTAIFGARDYSKK"
     gene            7689..8255
                     /gene="yggT"
                     /locus_tag="ACHOSC_RS16375"
                     /old_locus_tag="pgaptmp_003275"
     CDS             7689..8255
                     /gene="yggT"
                     /locus_tag="ACHOSC_RS16375"
                     /old_locus_tag="pgaptmp_003275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="osmotic shock tolerance protein YggT"
                     /protein_id="HGU4549168.1"
                     /translation="MNTLTFLLSTVIELYTMVLLLRIWMQWAHCDFYNPFSQFVVKVT
                     QPIIGPLRRVIPAMGPIDSASLLVAYILSFIKAIVLFKVVTFLPIIWIAGLLILLKTI
                     GLLIFWVLLVMAIMSWVSQGRSPIEYVLIQLADPLLRPIRRLLPAMGGIDFSPMILVL
                     LLYVINMGVAEVLQATGNMLLPGLWMAL"
     gene            8252..8542
                     /gene="yggU"
                     /locus_tag="ACHOSC_RS16380"
                     /old_locus_tag="pgaptmp_003276"
     CDS             8252..8542
                     /gene="yggU"
                     /locus_tag="ACHOSC_RS16380"
                     /old_locus_tag="pgaptmp_003276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417428.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF167 family protein YggU"
                     /protein_id="HGU4549169.1"
                     /translation="MSAVTVNDDGLVLRLYIQPKASRDSIVGLHGDEVKVAITAPPVD
                     GQANSHLVKFLGKQFRVAKSQVVIEKGELGRHKQIKIINPQQIPPEIAALIN"
     gene            8550..9143
                     /locus_tag="ACHOSC_RS16385"
                     /old_locus_tag="pgaptmp_003277"
     CDS             8550..9143
                     /locus_tag="ACHOSC_RS16385"
                     /old_locus_tag="pgaptmp_003277"
                     /EC_number="3.6.1.66"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047429 - nucleoside triphosphate
                     diphosphatase activity [Evidence IEA];
                     GO_process: GO:0009143 - nucleoside triphosphate catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="XTP/dITP diphosphatase"
                     /protein_id="HGU4549170.1"
                     /translation="MQKVVLATGNAGKVRELASLLSDFGLDIVAQTDLGVDSAEETGL
                     TFIENAILKARHAAKVTGLPAIADDSGLAVDVLGGAPGIYSARYSGEDATDLKNLQKL
                     LETLKDVPDDQRQARFHCVLVYLRHAEDPTPLVCHGSWPGVITREPAGTGGFGYDPIF
                     FVPSEGKTAAELTHEEKSAISHRGQALKLLLDALRNG"
     gene            9136..10272
                     /gene="hemW"
                     /locus_tag="ACHOSC_RS16390"
                     /old_locus_tag="pgaptmp_003278"
     CDS             9136..10272
                     /gene="hemW"
                     /locus_tag="ACHOSC_RS16390"
                     /old_locus_tag="pgaptmp_003278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0051989 - coproporphyrinogen dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM family heme chaperone HemW"
                     /protein_id="HGU4549171.1"
                     /translation="MVKLPPLSLYIHIPWCVQKCPYCDFNSHALKGEVPHDDYVQHLL
                     CDLDNDVAYAQGREVKTIFIGGGTPSLLSGPAMQTLLDGVRARLPLAADAEITMEANP
                     GTVEADRFVDYQRAGVNRISIGVQSFSEDKLKRLGRIHGPQEAKRAAKLASGLGLRSF
                     NLDLMHGLPDQSLEEALGDLRQAIELNPPHLSWYQLTIEPNTLFGSRPPVLPDDDALW
                     DIFEQGHQLLTAAGYQQYETSAYAKSSYQCQHNLNYWRFGDYIGIGCGAHGKVTFPDG
                     RILRTTKTRHPRGFMQGRYLESQRDVEAADKPFEFFMNRFRLLEAAPRVEFIAYTGLC
                     EDVIRPQLDEAIAQGYLTECADYWQITEHGKLFLNSLLELFLAE"
BASE COUNT         2416 a         2714 c         2715 g         2432 t
ORIGIN      
        1 aatctatgat gatataactc aattattttc atgcacttaa accataacta agataaatgt
       61 tagtgtaagc gattacactg atgtgatttg cttcacatct ttttacgtcg tactcaccta
      121 tcttaattca caataaaaaa taaccatatt ggagggcatc atgcctgacg ctaaaaaaca
      181 ggggcggtca aacaaggcaa tgactttttt cgtctgcttc cttgccgctc tggcgggatt
      241 actctttggc ctggatatcg gtgtaattgc tggcgcactg ccgtttattg cagatgaatt
      301 ccagattact tcgcacacgc aagaatgggt cgtaagctcc atgatgttcg gtgcggcagt
      361 cggtgcggtg ggcagcggct ggctctcctt taaactcggg cgcaaaaaga gcctgatgat
      421 cggcgcaatt ttgtttgttg ccggttcgct gttctctgcg gctgcgccaa acgttgaagt
      481 actgattctt tcccgcgttc tgctggggct ggcggtgggt gtggcctctt ataccgcacc
      541 actgtacctg tcagaaattg cgccggaaaa aattcgcggc agtatgatct cgatgtatca
      601 gttgatgatc actatcggga tcctcggtgc ttatctttct gataccgcct tcagctacac
      661 cggtgcatgg cgctggatgc tgggtgtgat tatcatcccg gcaattttgc tgctgattgg
      721 tgtcttcttc ctgccagaca gcccacgttg gtttgccgcc aagcgccgtt ttgttgatgc
      781 cgaacgcgtg ctgctacgcc tgcgtgacac cagcgcggaa gcgaaacgcg aactggatga
      841 aatccgtgaa agtttgcagg ttaaacagag tggctgggcg ctgtttaaag agaacagcaa
      901 cttccgccgc gcggtgttcc ttggcgtact gttgcaggta atgcagcaat tcaccgggat
      961 gaacgtcatc atgtattacg cgccgaaaat cttcgaactg gcgggttata ccaacactac
     1021 cgagcaaatg tgggggaccg tgattgtcgg cctgaccaac gtacttgcca cctttatcgc
     1081 aatcggcctt gttgaccgct ggggacgtaa accaacgcta acgctgggct tcctggtgat
     1141 ggctgctggc atgggcgtac tcggtacaat gatgcatatc ggtattcact ctccgtcagc
     1201 gcagtatttc gccatcgcca tgctgctgat gtttattgtc ggttttgcca tgagtgccgg
     1261 tccgctgatt tgggtactgt gctccgaaat tcagccgctg aaaggccgcg attttggcat
     1321 cacctgctcc accgccacca actggattgc caacatgatc gttggcgcaa cgttcctgac
     1381 catgctcaac acgctgggta acgccaacac cttctgggtg tatgcggctc tgaacgtact
     1441 gtttatcctg ctgacattgt ggctggtacc ggaaaccaaa cacgtttcgc tggaacatat
     1501 tgaacgtaat ctgatgaaag gtcgtaaact gcgcgaaatc ggcgctcacg attaatctcc
     1561 tcaggcttcc tcccatcgcg gaggaagcca cctcttgcag tcatcttttc ttcgctctat
     1621 cctctgccgc tatgaaaaca tcccgtctcc ctatcgccat ccagcaggcc gttatgcgcc
     1681 gcctgcggga aaaactcgcc caggccaacc tgaagctagg gcgtaactac ccggagccaa
     1741 aactctctta cacccagcgc ggaacctccg ccggaacagc ctggctggaa agctatgaaa
     1801 tccgcctcaa tcccgttttg ctgttggaaa acagtgaagc ttttattgaa gaagtggtac
     1861 cgcacgaact ggcacatttg ctggtatgga aacatttcgg ccgcgtagcg ccacatggca
     1921 aagagtggaa gtggatgatg gaaagcgtgc tgggcgttcc cgcccgtcgt acgcatcagt
     1981 tcgaactgca atccgtgcgt cgcaacacct tcccctaccg ctgcaagtgc caggagcatc
     2041 agcttaccgt acgccgccat aatcgcgtag ttcgtggcga ggccgtctat cgctgtgttc
     2101 actgcggtga acagctggtt gcgaaataac catctgaact atcaggaact ttcctgatct
     2161 ggctgattgc ataccaaaac aactttcgct acgttgctgg ctcgttttaa cacggagtaa
     2221 gtgatgtacc gttatttgtc tattgctgcg gtggtactga gcgcagcatt ttccggcccg
     2281 gcgttggccg aaggtatcaa tagtttttct caggcgaaag ccgcggcggt aaaagtccac
     2341 gctgacgcgc ccggtacgtt ttattgcgga tgtaaaatta actggcaggg caaaaaaggc
     2401 gttgttgatc tgcaatcgtg cggctatcag gtgcgcaaaa atgaaaaccg agccagccgc
     2461 gtagagtggg aacacgtcgt tcccgcctgg cagttcggtc accagcgcca gtgctggcag
     2521 gacggtggac gtaaaaactg cgctaaagat ccggtctatc gcaagatgga aagcgatatg
     2581 cataacctgc aaccgtcagt cggcgaggtg aatggcgatc gcggcaactt tatgtacagc
     2641 cagtggaatg gcggcgaagg ccagtacggt caatgcgcca tgaaggtcga tttcaaagaa
     2701 aaagctgccg aaccaccagc gcgtgcacgc ggtgccattg cacgcaccta cttctatatg
     2761 cgcgaccaat acaacctgac actctctcgc cagcaaacgc agctgttcaa cgcatggaac
     2821 aagatgtatc cggttaccga ctgggagtgc gagcgcgatg aacgcatcgc gaaggtgcaa
     2881 ggcaatcata atccgtatgt gcaacgcgct tgccaggcgc gaaagagcta acctacacta
     2941 gcgggactct ttttgttaac ccctacccca cgcgtacaac cgcgtgggga gacgacgcgg
     3001 atttttaact atgcgtatcc cccgcattta tcatcctgaa ccactgacca gccattctca
     3061 catcgcgctt tgcgaagatg ccgccaacca tatcgggcgc gtactgcgca tggggccggg
     3121 gcaggcgttg caattgtttg acggtagcaa ccaggtcttt gacgccgaaa ttaccagcgc
     3181 cagcaaaaaa agcgtggaag tgaaggtgct ggaaggcaag atcgacgatc gcgaatctcc
     3241 gctgcatatt cacctcggtc aggtgatgtc gcgtggtgaa aaaatggaat ttacgatcca
     3301 gaaatcgatc gaactcggtg taagcctcat tacgccactt ttttctgagc gctgcggcgt
     3361 taaactggat agtgaacgtc tgaacaagaa gctccagcag tggcagaaga ttgcaattgc
     3421 tgcctgtgag cagtgtggtc gtaaccgggt gccggaaatc cgcccagcga tggatctgga
     3481 agcctggtgt gcagagcagg atgaaggact gaaactgaat cttcacccgc gcgccagtaa
     3541 cagcatcaat acgttgccgt taccggttga acgcgtccgc ctgctgattg gcccggaagg
     3601 cggtttatcg gcagatgaaa ttgccatgac tgcccgctat caatttactg atatcctgtt
     3661 gggacctcgc gttttgcgta cagagacaac tgcgctcacc gccattaccg cgctacaagt
     3721 acgatttggc gatttgggct aacggagaag aataatgatc aagctcggca tcgtgatgga
     3781 ccccatcgca aacatcaaca tcaagaaaga ttccagtttc gctatgttgc tggaagcaca
     3841 gcgtcgtggt tacgaacttc actatatgga gatgggcgat ctgtatctga tcaatggcga
     3901 agcccgcgcc catacccgca cgctgaacgt gaagcagaac tacgaagagt ggttttcgtt
     3961 cgtcggtgaa caggatctgc cgctggccga tctcgatgtg atcctgatgc gtaaagaccc
     4021 gccgtttgat accgagttta tctacgcgac ctatattctg gaacgtgccg aagagaaagg
     4081 gacgctgatc gttaacaagc cgcagagcct gcgcgactgt aacgagaaac tgtttaccgc
     4141 ctggttctct gacttaacgc cagaaacgct ggttacgcgc aataaagcgc agctaaaagc
     4201 gttctgggag aaacacagcg acatcattct taagccgctg gacggtatgg gcggcgcgtc
     4261 gattttccgc gtgaaagaag gcgatccaaa cctcggcgtg attgccgaaa ccctgactga
     4321 gcatggcact cgctactgca tggcgcaaaa ttacctgcca gccattaaag atggcgacaa
     4381 acgcgtgctg gtggtggatg gcgagccggt accgtactgc ctggcgcgta ttccgcaggg
     4441 gggcgaaacc cgtggcaatc tggctgccgg tggtcgcggt gaacctcgtc cgctgacgga
     4501 aagtgactgg aaaatcgccc gtcagatcgg gccgacgttg aaagaaaaag ggctgatttt
     4561 tgttggtctg gatatcatcg gcgaccgtct gactgaaatt aacgtcacca gcccaacctg
     4621 tattcgtgag attgaagcag agtttccggt gtcgatcacc ggaatgttaa tggatgccat
     4681 cgaagcacgt ttacagcagc agtaacccac cttagcgaga aggatctcgt tgagactctg
     4741 agtgacagcg cccttctttc cacgcatact gggcgctgtt gcttttttga accaggaaac
     4801 agaacctctg acaatgaatt tacagcatca ctttcttatt gccatgcctg ctctccagga
     4861 tccgattttc cgtcgttccg tggtctacat ttgcgaacat aataccaatg gtgcaatggg
     4921 gatcatcgtc aacaagccgc tggaaaatct caaaattgaa gggattctgg aaaagctgaa
     4981 gatcacgccg gagccgcgtg atgaatcaat ccgtctggat aaaccggtta tgctcggcgg
     5041 tccgctggct gaagatcgcg ggtttatttt gcatactccg ccctccaatt ttgcttccag
     5101 cattcgcatt tcagacaaca cagtgatgac cacttcccgc gatgtgctgg aaacgctcgg
     5161 caccgataaa caaccgtctg acgtattggt ggctctgggt tatgcctcct gggaaaaagg
     5221 tcaactggaa caagaaattc tcgataacgc gtggctaacg gccccggcgg atctgaatat
     5281 tctgttcaaa acgccgattg ccgaccgctg gcgcgaggcg gcaaaactga ttggtgtgga
     5341 tattctcacc atgcctggtg tggcagggca cgcctgatga gtggaacctt actcgccttc
     5401 gacttcggca ccaaaagcat tggcgtagcg gtcggccaac gcattaccgg cactgctcgc
     5461 cctttgcctg caattaaagc acaggacggt acgccggact ggaacctcat cgaacgttta
     5521 ctgaaagagt ggcagccgga cgaaatcatc gtcggtttgc cgctgaatat ggacggcacc
     5581 gagcaaccat tgactgccag agcgcgtaaa tttgccaacc gtattcatgg tcgtttcggt
     5641 gttgaagtaa agctccatga cgaacgtctt agcaccgtgg aagcccgttc cggtctgttt
     5701 gaacagggcg gctatcgggc gctcaacaaa ggcaaagtcg actctgcctc tgcggttatt
     5761 attctcgaaa gctatttcga gcagggatat taaggcgatc taaaagcctg gcggatagtc
     5821 gttaccgcca tcttgccgga tgcggcgtga acgccttatc cagcacacat ctggcagcgg
     5881 ctgtaggtct gataagacgc gttagcgtcg catcagacat ttattgcctt tataggccct
     5941 ataaacttgc gcatcgggca tggcaacgtc acaaacgccc ttcccccacc cgctgctgat
     6001 aactctgctg aaacgttaac atccccacct gctgcccggt ttgaataaca tgcggtaact
     6061 ggtgggtttt cccttcgcga atcaaattcc ccaccgcggg agtgttaatc aataattcaa
     6121 acagcgccac gcgtccttcc tgtttatcca cttccagctt ttgtgacaac acggcccgta
     6181 aactacctgc cagttgatta cgtacgggat ctttttcctg cgccggaaat gaatccacca
     6241 gtcgctcaac tgcctgcgcg gcaccacgcg tatgtaatgt tgccagcacc aaatgcccgg
     6301 tttctgccgc cgtcagtgcc agacggattg tctcgctgtc acgcagctct ccgagcaaaa
     6361 tcacatcagg atcttcccgc aatgcggccc gcaatcccga tgcgaacgtc atacagtgca
     6421 aaccaatttc ccgctgctgg atcaaacatc gctggctggc atagatatat tcaacaggat
     6481 cttccagcgt cagaatatgc gcatcggcat gttgatagag atagccaacc atcgccgcta
     6541 gcgtggtaga tttgccgctc cccgtcgccc ccgtcaccag aatcaggcca ttctcgctct
     6601 tgagtaattc cggcaatacc gttggtgcgc caagctgttc gagctgtgga cagtgcgaag
     6661 gtaacaaccg taatgccaga gaaatacctt gccgttgcgc gaacgcactg ccacgcaacc
     6721 gctggttttc cgccagcgac acggcaaaat ccagctggcc attttccagc aatattgccc
     6781 gctgatcgtc atccagccac tcccgcagta gctcttcgac gtccggcgca tcaaacggcg
     6841 cagcttccat tagcccgcga atgcgccatc gtgcggacca ggcgctgcac aggtgtagat
     6901 ccgagacgtt atgctttaca ctaagggcca caatttcttc catattcata ctaagatcct
     6961 cggaaaatga acgatattgc gcataacctg gcacaggtcc gggacaaaat ctcagcggct
     7021 gcaacgcgtt gcggccgttc tccagaagaa attacgctgc ttgcagtcag taaaacaaaa
     7081 cctgcgagcg ccatcgcaga agccattgat gccgggcagc gtcaatttgg tgaaaactac
     7141 gttcaggaag gggtagataa aattcgccac tttcaggaac tgggcgtaac aggattagaa
     7201 tggcatttta ttggcccgtt gcagtctaat aaaagccgcc tggtggcaga gcatttcgac
     7261 tggtgtcata ccatcgaccg tttgcgcatc gctacccgcc tcaacgatca gcgcccggca
     7321 gaacttcccc ctcttaacgt actgattcaa attaatatta gtgatgaaaa cagtaagtcc
     7381 gggattcaac tggcagaact ggacgagctg gcagctgcgg tcgctgaact accgcgttta
     7441 cgtctgcgcg ggctgatggc aatccctgca cctgagtcag aatatgtaag gcagtttgaa
     7501 gttgcacgcc aaatggctgt agcatttgcc ggactgaaaa cgcgctaccc gcatatcgac
     7561 acgctctctc tgggaatgtc ggacgatatg gaagccgcca ttgcggcagg tagcacgatg
     7621 gttcgtatcg gcactgcaat ttttggtgcg cgtgattact ctaaaaaata aggaattaaa
     7681 ggaacgccat gaatacgttg actttcctgc tttcaacggt cattgagctg tataccatgg
     7741 tgctgttatt acgcatctgg atgcagtggg ctcattgtga tttttacaac cccttctcac
     7801 agtttgtagt gaaggtaacg cagccaatta tcgggccact gcgccgcgtt attccggcaa
     7861 tgggccccat tgacagcgcc tcgctgctgg ttgcctatat tctcagtttt atcaaagcca
     7921 tcgtgctgtt taaagtggtg accttcctgc caatcatctg gattgccggt ttactgattc
     7981 tgctgaaaac catcggcctg ctgattttct gggtcctgct ggtgatggcg attatgagct
     8041 gggtaagcca gggtcgtagc ccgattgaat acgtgctgat tcagctggcc gatccgctgc
     8101 tgcgcccgat tcgccgcctg ctaccggcaa tgggtgggat tgatttctcg ccgatgatcc
     8161 tcgttctgct gctgtatgtc atcaatatgg gtgtcgcaga agtattacag gcaaccggaa
     8221 acatgctgct gccggggctg tggatggcgt tatgagtgcc gtaacagtta atgatgacgg
     8281 tctggtttta cggctctata ttcagccgaa agccagccgt gattctattg tcggtttaca
     8341 tggcgacgaa gttaaagtcg ccattaccgc gccgccggtt gacggtcagg ccaacagtca
     8401 tctggtgaag tttctcggta agcaattccg ggttgccaaa agccaggtgg tgattgaaaa
     8461 aggcgaactt ggccgccaca aacaaattaa aatcattaat ccgcaacaaa tcccgccaga
     8521 aatcgcggcg ttaattaatt aggtatccta tgcaaaaagt tgtcctcgca accggcaatg
     8581 ccggtaaagt gcgtgagctg gcgtcgctgc ttagcgactt cggtcttgat atcgtggccc
     8641 aaacagacct cggcgtagat tccgctgaag aaaccggcct gacctttatc gaaaacgcga
     8701 ttctgaaagc gcgccatgcg gcaaaagtga ccggtttacc ggcaattgcc gacgactctg
     8761 gtctggcggt agatgtgctt ggcggcgcgc cgggcattta ctccgcgcgt tattccggtg
     8821 aagacgcgac cgatctaaag aatctgcaaa aactgctgga aacactgaaa gacgtaccgg
     8881 atgaccaacg tcaggcgcgt ttccactgcg tgctggtata tctgcgtcac gcggaagatc
     8941 cgactccgct ggtatgccac ggtagctggc cgggcgtgat tactcgtgaa ccagcgggca
     9001 ctggtggctt tggttatgat ccaatcttct tcgtaccttc cgaagggaaa accgccgccg
     9061 aactgaccca cgaagaaaag agcgccattt cccaccgtgg tcaggcattg aaactgctgc
     9121 tggacgcttt acgtaatggt taaattaccg ccgctgagtc tctacattca catcccgtgg
     9181 tgcgtgcaga aatgcccgta ctgcgatttc aactctcacg cgttgaaagg agaagtgccg
     9241 cacgacgatt acgttcagca tctgctttgc gatctggaca acgatgtggc ttacgctcag
     9301 ggccgtgaag taaagacaat ctttattggc ggtggtacgc cgagcctgct ttccggcccg
     9361 gcgatgcaaa cgctgctgga cggcgtgcgt gcgcgtttgc cgctggcagc ggatgcagaa
     9421 attactatgg aagcgaaccc tggtacggta gaagccgatc gctttgtcga ttatcagcgt
     9481 gctggtgtga accgcatctc tattggtgtg cagagtttta gcgaagacaa gctgaaacga
     9541 cttgggcgta ttcatggccc gcaagaagcg aaacgcgcgg caaagctggc gagcgggctg
     9601 gggctgcgta gctttaacct cgatttgatg catggactac cggatcaatc actggaagag
     9661 gcgcttggcg atctacgcca ggccattgaa ctgaatccgc cgcatctttc ctggtatcaa
     9721 ctgaccatcg aacctaatac gctgtttggt tcacgcccgc cggtactgcc ggacgacgac
     9781 gcgctatggg atatattcga acaggggcat cagttattaa ccgcagcggg ttatcagcaa
     9841 tatgaaactt ccgcttacgc caaatccagt tatcagtgcc agcacaatct caactactgg
     9901 cgctttggcg actacatcgg tattggctgc ggcgcgcacg gcaaagtgac cttcccggat
     9961 gggcgcattc tgcgtaccac taaaacgcgt catccgcgtg gttttatgca aggaaggtat
    10021 ctggaaagcc agcgtgatgt cgaagccgca gataagccgt ttgagttctt tatgaatcgc
    10081 ttccgtctgc tggaggccgc gccgcgcgtg gagtttattg cgtataccgg gctttgcgaa
    10141 gatgtgattc gcccacagtt agacgaggcg attgcccagg gttatctcac cgaatgtgcg
    10201 gattactggc agataacgga acatggtaaa ttattcttaa actcacttct tgaattattc
    10261 ctcgctgagt aatcatt
//
LOCUS       DAZKHF020000162        10272 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2201S31241
            SAMN43267800-rid25106413.denovo.163, whole genome shotgun sequence.
ACCESSION   DAZKHF020000162 DAZKHF020000000
VERSION     DAZKHF020000162.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267800
            Sequence Read Archive: SRR30307263
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 10272)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10272)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 10272)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350581.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350581.2
            Genome Coverage       :: 151x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:21:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,652
            CDSs (total)                      :: 5,557
            Genes (coding)                    :: 5,320
            CDSs (with protein)               :: 5,320
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 237
            CDSs (without protein)            :: 237
            Pseudo Genes (ambiguous residues) :: 0 of 237
            Pseudo Genes (frameshifted)       :: 102 of 237
            Pseudo Genes (incomplete)         :: 130 of 237
            Pseudo Genes (internal stop)      :: 52 of 237
            Pseudo Genes (multiple problems)  :: 41 of 237
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10272
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267800-rid25106413.denovo.163"
                     /serotype="O 26:H11"
                     /isolate="2201S31241"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-01"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            1..1629
                     /gene="yqiK"
                     /locus_tag="ACHOSC_RS16395"
                     /old_locus_tag="pgaptmp_003279"
                     /pseudo
     CDS             1..1629
                     /gene="yqiK"
                     /locus_tag="ACHOSC_RS16395"
                     /old_locus_tag="pgaptmp_003279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417523.1"
                     /note="internal stop; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="flotillin family protein"
     gene            1868..1927
                     /locus_tag="ACHOSC_RS16400"
                     /old_locus_tag="pgaptmp_003280"
     CDS             1868..1927
                     /locus_tag="ACHOSC_RS16400"
                     /old_locus_tag="pgaptmp_003280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Ibs family toxin"
                     /protein_id="HGU4549172.1"
                     /translation="MMKLVIILIVLLIVSFAAY"
     gene            2244..2303
                     /gene="ibsE"
                     /locus_tag="ACHOSC_RS16405"
                     /old_locus_tag="pgaptmp_003281"
     CDS             2244..2303
                     /gene="ibsE"
                     /locus_tag="ACHOSC_RS16405"
                     /old_locus_tag="pgaptmp_003281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin IbsE"
                     /protein_id="HGU4549173.1"
                     /translation="MMKFVIILVVLLLLSFPTY"
     gene            complement(2423..3856)
                     /gene="hldE"
                     /locus_tag="ACHOSC_RS16410"
                     /old_locus_tag="pgaptmp_003282"
     CDS             complement(2423..3856)
                     /gene="hldE"
                     /locus_tag="ACHOSC_RS16410"
                     /old_locus_tag="pgaptmp_003282"
                     /EC_number="2.7.1.167"
                     /EC_number="2.7.7.70"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     D-glycero-beta-D-manno-heptose-7-phosphate
                     kinase/D-glycero-beta-D-manno-heptose 1-phosphate
                     adenylyltransferase HldE"
                     /protein_id="HGU4549174.1"
                     /translation="MKVTLPEFERAGVMVVGDVMLDRYWYGPTSRISPEAPVPVVKVN
                     TIEERPGGAANVAMNITSLGANARLVGLTGIDDAARALSKSLADVNVKCDFVSVPTHP
                     TITKLRVLSRNQQLIRLDFEEGFEGVDPQPLHERINQALSSIGALVLSDYAKGALASV
                     QQMIQLARKAGVPVLIDPKGTDFERYRGATLLTPNLSEFEAVVGKCKTEEEIVERGMK
                     LIADYELSALLVTRSEQGMSLLQPGKAPLHMPTQAQEVYDVTGAGDTVIGVLAATLAA
                     GNSLEEACFFANAAAGVVVGKLGTSTVSPIELENAVRGRADTGFGVMTEEELKLAVAA
                     ARKRGEKVVMTNGVFDILHAGHVSYLANARKLGDRLIVAVNSDASTKRLKGDSRPVNP
                     LEQRMIVLGALEAVDWVVSFEEDTPQRLIAGILPDLLVKGGDYKPEEIAGSKEVWANG
                     GEVLVLNFEDGCSTTNIIKKIQQDKKG"
     gene            complement(3904..6744)
                     /gene="glnE"
                     /locus_tag="ACHOSC_RS16415"
                     /old_locus_tag="pgaptmp_003283"
     CDS             complement(3904..6744)
                     /gene="glnE"
                     /locus_tag="ACHOSC_RS16415"
                     /old_locus_tag="pgaptmp_003283"
                     /EC_number="2.7.7.42"
                     /EC_number="2.7.7.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008882 - [glutamate-ammonia-ligase]
                     adenylyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional [glutamate--ammonia
                     ligase]-adenylyl-L-tyrosine
                     phosphorylase/[glutamate--ammonia-ligase]
                     adenylyltransferase"
                     /protein_id="HGU4549175.1"
                     /translation="MKPLSSPLQQYWQTVVERLPEPLAEESLSAQAKSVLTFSDFVQD
                     SVIVHPEWLTELESQPPQADEWQHYAAWLQEALSNVSDEAGLMRELRLFRRRIMVRIA
                     WAQTLALVTEESILQQLSHLAETLIVAARDWLYDACCREWGTPCNAQGEAQPLLILGM
                     GKLGGGELNFSSDIDLIFAWPEHGCTQGGRRELDNAQFFTRMGQRLIKVLDQPTQDGF
                     VYRVDMRLRPFGESGPLVLSFSALEDYYQEQGRDWERYAMVKARIMGDSEGVYANELR
                     AMLRPFVFRRYIDFSVIQSLRNMKGMIAREVRRRGLTDNIKLGAGGIREIEFIVQVFQ
                     LIRGGREPSLQSRSLLPTLSVIAALHLLSENDAEQLRVAYLFLRRLENLLQSINDEQT
                     QTLPSDELNRARLAWAMDFADWPQLTGALTAHMTNVRRVFNELIGDDESETQEESLSE
                     QWRELWQDALQEDDTTPVLAHLSEDDRKQVLTLIADFRKELDKRTIGPRGRQVLDHLM
                     PHLLSDVCAREDAAVTLSRITALLVGIVTRTTYLELLSEFPAALKHLISLCAASPMIA
                     SQLARYPLLLDELLDPNTLYQPTATDAYRDELRQYLLRVPEDDEEQQLEALRQFKQAQ
                     LLRIAAADIAGTLPVMKVSDHLTWLAEAMIDAVVQQAWVQMVARYGKPNHLNDREGRG
                     FAVVGYGKLGGWELGYSSDLDLIFLHDCPMDAMTDGEREIDGRQFYLRLAQRIMHLFS
                     TRTSSGILYEVDARLRPSGAAGMLVTSAEAFADYQKNEAWTWEHQALVRARVVYGDPQ
                     LTAHFDAVRREIMTLPREGKTLQTEVREMREKMRAHLGNKHRDRFDIKADEGGITDIE
                     FITQYLVLRYAHEKPKLTRWSDNVRILELLAQNDIMEEQEAMALTRAYTTLRDELHHL
                     ALQELPGHVPEDCFTAERELVRASWQKWLVEE"
     gene            complement(6767..8068)
                     /gene="ygiF"
                     /locus_tag="ACHOSC_RS16420"
                     /old_locus_tag="pgaptmp_003284"
     CDS             complement(6767..8068)
                     /gene="ygiF"
                     /locus_tag="ACHOSC_RS16420"
                     /old_locus_tag="pgaptmp_003284"
                     /EC_number="3.6.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic triphosphatase"
                     /protein_id="HGU4549176.1"
                     /translation="MAQEIELKFIVNHSAVEALRDHLNTLGGEHHDPVQLLNIYYETP
                     DNWLRGHDMGLRIRGENGRYEMTMKVAGRVTGGLHQRPEYNVALSEPTLDLAQLPTEV
                     WPNGELPADLASRVQPLFSTDFYREKWLVAVDDSRIEIALDQGEVKAGEFAEPICELE
                     LELLSGDTRAVLKLANQLVSQTGLRQGSLSKAARGYHLAQGNPAREIKPTTILHVAAK
                     ADVEQGLEAAFELALAQWQYHEELWVRGNDAAKEQVLAAIGLVRHALMLFGGIVPRKA
                     STHLRDLLTQCEATIASAVSAVTAVYSTETAMAKLALTEWLVSKAWQPFLDAKAQGKI
                     SDSFKRFADIHLSRHAAELKSVFCQPLGDRYHDQLPRLTRDIDSILLLAGYYDPVVAQ
                     AWLENWQGLRHAIATGQRIEIEHFRNEANNQEPFWLHSGKR"
     gene            8310..8930
                     /gene="ygiM"
                     /locus_tag="ACHOSC_RS16425"
                     /old_locus_tag="pgaptmp_003285"
     CDS             8310..8930
                     /gene="ygiM"
                     /locus_tag="ACHOSC_RS16425"
                     /old_locus_tag="pgaptmp_003285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR04211 family SH3 domain-containing protein"
                     /protein_id="HGU4549177.1"
                     /translation="MPKLRLIGLTLLALSATAVSHAEETRYVSDELNTWVRSGPGDHY
                     RLVGTVNAGEEVTLLQTDANTNYAQVKDSSGRTAWIPLKQLSTEPSLRSRVPDLENQV
                     KTLTDKLTNIDNTWNQRTAEMQQKVAQSDSVINGLKEENQKLKNELIVAQKKVDAASV
                     QLDDKQRTIIMQWFMYGGGVLGLGLLLGLVLPHLIPTRKRKDRWMN"
     gene            8994..10232
                     /gene="cca"
                     /locus_tag="ACHOSC_RS16430"
                     /old_locus_tag="pgaptmp_003286"
     CDS             8994..10232
                     /gene="cca"
                     /locus_tag="ACHOSC_RS16430"
                     /old_locus_tag="pgaptmp_003286"
                     /EC_number="2.7.7.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fused tRNA nucleotidyltransferase/2',3'-cyclic
                     phosphodiesterase/2' nucleotidase/phosphatase Cca"
                     /protein_id="HGU4549178.1"
                     /translation="MKIYLVGGAVRDALLGLPVKDRDWVVVGSTPQEMLDAGYQQVGR
                     DFPVFLHPLTHEEYALARTERKSGSGYTGFTCYAAPDVTLEDDLKRRDLTINALAQDD
                     NGEIIDPYNGLGDLQNRLLRHVSPAFGEDPLRVLRVARFAARYAHLGFRIADETLTLM
                     REMTHAGELEHLTPERVWKETESALTTRNPQVFFQVLRDCGALRVLFPEIDALFGVPA
                     PARWHPEIDTGIHTLMTLSMAAMLSPQVDVRFATLCHDLGKGLTPPELWPRHHGHGPA
                     GVKLVEQLCQRLRVPNEIRDLARLVAEFHDLIHTFPMLNPKTIVKLFDSIDAWRKPQR
                     VEQLALTSEADVRGRTGFESADYPQGRWLREAWEVAQSVPTKAVVEAGFKGVEIREEL
                     TRRRIAAVASWKEQRCPKPD"
BASE COUNT         2439 a         2868 c         2658 g         2307 t
ORIGIN      
        1 atgtttaccg cgattattgc cgtatgcatt ctgtttatta ttggaattat tttcgccagg
       61 ctctatcgtc gcgcttcggc agagcaagct tttgttcgta ctggtttagg tgggcaaaaa
      121 gtggtgatga gcggtggcgc aatcgtgatg ccgatctttc atgaaataat ccccatcaat
      181 atgaatactc tgaagctgga agtcagccgc tcaaccattg atagcctgat tacgaaagat
      241 cgtatgcgtg tcgatgtagt agtcgctttc tttgtacggg taaaaccgtc tgtagaaggc
      301 atcgccactg cggcgcagac gttggggcaa cgcacgctat caccagaaga cttacgtatg
      361 ttggttgaag ataaatttgt cgatgccctc cgtgcaacag ctgcacaaat gaccatgcat
      421 gagttacagg atacccgcga gaacttcgtg cagggagtac aaaatactgt cgcagaagac
      481 ctgtcgaaaa acggtcttga gctggaaagc gtttcactta ccaactttaa ccagacctcg
      541 aaagaacatt tcaatccgaa caatgccttt gacgccgaag gtttaaccaa actgactcag
      601 gagacggagc gccgtcgtcg cgaacgtaac gaagttgaac aggatgtaga agttgcggtg
      661 cgtgagaaaa accgtgatgc actttcgcgc aagttggaga ttgaacaaca agaagcgttt
      721 atgacgcttg agcaggagca gcaggttaaa acccgtactg ccgaacagaa tgcacgtatt
      781 gcggcttttg aagctgaacg tcgtcgtgaa gcagagtaga cgcgaattct ggctgaacga
      841 caaattcagg aaacggagat cgagcgcgag caggccgtcc gctcaagaaa ggttgaggct
      901 gaacgtgaag ttcgtattaa agagatcgaa caacagcagg tcaccgaaat cgccaaccag
      961 acgaaatcga tcgctattgc cgccaaatcg gaacaacagt cccaggcaga agcgcgtgct
     1021 aaccttgcac tcgcagaagc agtaagtgcg caacaaaacg tagaaaccac tcgccagacc
     1081 gcagaagccg atcgtgctaa acaagttgcc ctaatcgctg ccgcgcagga tgcagaaacc
     1141 aaagcggttg aactgaccgt gcgggcgaaa gcagaaaaag aagccgcaga gatgcaggcg
     1201 gcggctatcg ttgagttagc cgaagctaca cgtaaaaagg gtctggcgga agcagaagca
     1261 caacgtgcgc tgaacgatgc tatcaacgta ctttctgatg aacaaaccag ccttaaattc
     1321 aaactggcct tgttgcaggc gctacctgcg gtaatagaaa aatccgttga gccgatgaaa
     1381 tctattgacg gtatcaagat tattcaggtc gatggtctga atcgtggcag cgctgcgggt
     1441 gatgcaaaca cgggtaatgt ggggggcgga aacctggcgg aacaagcatt atcagccgct
     1501 ctctcttacc gcacacaggc accgctgatt gactccttgc tcaatgaaat tggcgtttca
     1561 ggcggctcac tggcggcatt gacttcaccc ttaacctcaa caactcccgt cgccgaaaac
     1621 gtagaataaa ttttcagcta tgaacgtccc tgtcacaaaa agccggggat tttttatatc
     1681 tgcgttccgc taaaaactgc aaatgatcag gtcgttgcaa cgttttgcgt gaccgctcgg
     1741 ggaaggcaaa actgcctctg ggaaagcgtt gcgcgaggtc cggcgctcat caacaatcgg
     1801 ggagtcagca aagggctgaa acgggaaagc ccctccctag gaaggggcct tacagaagga
     1861 aagggttatg atgaagctcg tcatcatact gattgtgttg ttaatcgtaa gtttcgcagc
     1921 ttattaacag ccaatcagag gggggagaaa tcctccctca cccttgttcc tttactttag
     1981 gttgaaaaaa aatcagcgtc aagaaggttt cattaacaca tatccctggc ccaaaaagcc
     2041 ggggattttt tatatctgcg ttccgctaaa aggtgcaaat gctcaggacg ttgcagcgtt
     2101 ttgcgtgacc gctcggggaa ggcaaaactg cttcagggaa agcgttgcgc ggggtccggc
     2161 gctcatcaac aatcggggag tcagcaaggg gctgaaacgg gaaagcccct cccgaggaag
     2221 gggccttata gaaggaaagg gttatgatga agttcgtcat catactggtt gtgttgttac
     2281 tgttaagttt cccgacttac taacaactga tcagaggggg gagaaatcct cccttaccct
     2341 tgttccttta ctttaggttg aaaaaacaac agcgtcaata ggcctgcctg ctacgaagcg
     2401 agatctgtga accgctttcc ggttagcctt ttttatcctg ttggatcttc ttgatgatgt
     2461 tagtggtcga gcaaccgtct tcgaagttga gcaccagcac ttcgccgccg ttggcccaga
     2521 cttctttact cccggcgatc tcttctggtt tatagtcgcc gcccttcacc agtaaatctg
     2581 gcaggatccc ggcgatcaag cgctgcggcg tgtcttcttc aaacgacacc acccagtcga
     2641 ccgcttccag tgcgcccagc acaatcatac gctgttcgag cgggttaacg gggcgggaat
     2701 cccctttcag ccgtttggtg gaggcatcgc tgttcacagc gacaatcaga cggtcaccca
     2761 gtttgcgagc atttgccaga taggagacgt gcccggcgtg caggatgtca aagacaccgt
     2821 tggtcattac caccttttcg ccgcgtttac gcgctgccgc tacagcaagc ttcagttcct
     2881 cttcggtcat cacaccaaag ccggtatctg cgcgcccacg cactgcattt tccagctcga
     2941 tcggcgaaac cgtggaggtt cccagtttgc cgaccaccac gccagccgcc gcattggcaa
     3001 agaagcaggc ttcttccagc gaattacccg ctgccagcgt tgccgccagg acgccaatca
     3061 ccgtgtcgcc cgcaccggta acgtcataca cttcctgcgc ctgggttggc atatgcagcg
     3121 gcgctttacc cggttgcagc agcgacatac cctgttcgga acgggtcact aacagagccg
     3181 agagttcgta atcggcaatc agtttcatgc cgcgctcaac aatctcttct tcggtcttac
     3241 atttaccgac aacagcttca aattccgaga gattcggtgt cagcagcgta gcgccgcggt
     3301 agcgctcaaa atcggtacct tttggatcaa tcagcaccgg aacgcccgct ttacgcgcca
     3361 gttggatcat ctgctgtacg cttgccagcg cacctttggc gtagtcagaa agcaccagcg
     3421 cgccaatcga actcagcgcc tgattaatcc gctcgtgcag cggctgcgga tcaacacctt
     3481 cgaaaccttc ttcaaaatcc aggcggatca gctgttggtt gcgggaaagt acccgtaatt
     3541 tggtgatagt cggatgcgtc ggtacagaaa cgaagtcgca tttgacgttg acgtcggcca
     3601 gagatttact cagcgcacgc gccgcatcgt caatgcccgt cagcccgacc aggcgagcat
     3661 tggcaccgag agaagtgatg ttcattgcca cgttagccgc gccgcccgga cgttcttcga
     3721 tggtattgac tttaaccacg ggcaccggcg cttccggcga gatacggctg gtgggaccgt
     3781 accagtaacg atccagcatc acatcaccaa ccaccatcac tcctgcacgt tcaaactctg
     3841 gcagcgttac tttcattcct gtctcctgag agattcaaaa tttgcgcgcg ataataccat
     3901 acttcattct tccaccagcc acttctgcca gcttgcccgc accagttcac gctctgcggt
     3961 gaagcaatcc tccggcacat ggcccggcaa ttcctgtaat gccagatgat gaagttcatc
     4021 gcgcagcgta gtatacgcac gggtcagcgc catcgcttcc tgctcttcca taatgtcgtt
     4081 ttgcgccagt agttccagaa tgcgcacgtt gtctgaccag cgcgttaact tcggtttttc
     4141 atgagcgtag cgcaacacca gatattgggt aataaattcg atatcggtaa ttccgccttc
     4201 atcagctttg atatcaaagc gatcgcgatg tttattgccg agatgagcgc gcattttctc
     4261 gcgcatttcc cgcacttccg tttgcagagt tttaccttca cgcggcagcg tcataatctc
     4321 gcgacgcacc gcgtcaaagt gcgcggtgag ctgcggatcg ccgtacacta cccgcgcacg
     4381 caccagcgcc tgatgttccc acgtccaggc ctcgtttttc tgataatcgg caaatgcttc
     4441 tgcggatgtc accagcattc ccgcggcccc ggacgggcgt agccgtgcat ccacttcata
     4501 caaaatgccg gaagaggtac gcgtactgaa cagatgcata atgcgttgcg ccagacgcag
     4561 ataaaactgt cgcccgtcga tttcccgctc gccgtcagtc atcgcatcca ttgggcaatc
     4621 atggaggaag atcaggtcaa gatcggaact gtagcctaac tcccagccgc ccagcttgcc
     4681 gtagccgacc accgcaaaac cacggccttc gcgatcgttg aggtgatttg gcttaccgta
     4741 gcgggccacc atttgaaccc acgcctgctg aacgacggca tctatcatag cctctgccag
     4801 ccaggttaag tgatcgctca ctttcatcac cggtagcgta ccagcgatat ccgctgcggc
     4861 gatgcgtaac agctgtgcct gtttgaactg acgcagcgcc tcaagctgtt gctcttcgtc
     4921 atcttccggc acgcgcagca aatactggcg caactcatcg cggtaggcat cggtcgccgt
     4981 cggctggtaa agggtgtttg gatcgagcaa ttcatccagc aataatggat aacgcgccag
     5041 ttggctggca atcatcggcg acgcggcaca cagagaaatc aaatgtttaa gcgccgcggg
     5101 gaattcactg agcaattcta aataggtggt gcgggtaaca atccccacca gcaaggcggt
     5161 aatgcgcgac agcgtaacgg cagcgtcttc acgcgcacag acatcactta gcagatgcgg
     5221 catcagatgg tcgagcacct gacgtcctcg cggcccgatg gtgcgcttat ccagctcttt
     5281 gcggaaatcg gcaatcagcg ttagcacctg tttgcgatca tcctcgctaa gatgcgccag
     5341 cactggcgta gtgtcatctt cctgcaacgc atcctgccac agctcacgcc actgttccga
     5401 cagcgactct tcctgagttt cactttcgtc gtcgccaatc aattcattaa acacccggcg
     5461 cacattggtc atatgtgcgg tcagcgcccc ggtcagttgc ggccagtcag caaagtccat
     5521 cgcccacgcc agccgcgcac ggttaagctc atcagaagga agcgtctggg tttgttcgtc
     5581 gttaatgctt tgcagcaggt tttccagacg ccgcaggaac agatacgcca ctcgcaattg
     5641 ttcagcatcg ttttcagaaa gcagatgcag cgcggcaatg acgctgagcg ttggcagtaa
     5701 agagcgcgac tgcagcgacg gttcgcgccc accgcgaatg agctggaaca cctgaacgat
     5761 aaattcaatt tcgcgaatgc cgcctgcgcc gagtttgata ttgtcggtca aaccacgtcg
     5821 acgcacttca cgggcaatca tccctttcat gttgcgcagc gactgaatca cgctgaaatc
     5881 gatgtaacga cggaaaacaa acgggcgcag catcgcacgc aactcgttag catagacgcc
     5941 ttcgctatcg cccataatcc gcgccttgac catcgcgtaa cgctcccagt cgcgcccctg
     6001 ctcctggtaa taatcctcca acgcggaaaa gctcagcacc agcgggccac tttcgccaaa
     6061 cggacgcagc cgcatatcca cgcgatagac gaagccatcc tgcgttggtt gatccaacac
     6121 tttaatcagc cgctgcccca tgcgggtaaa aaactgcgcg ttatccagtt cccggcgtcc
     6181 gccctgcgta caaccatgtt ccggccaggc aaaaatcaga tcgatatctg aggagaaatt
     6241 cagttcccca ccgcccagct tacccatgcc taaaatcagc agcggttgcg cttcgccctg
     6301 cgcattgcac ggcgttcccc actcgcggca gcaagcgtca tacagccagt cacgcgcggc
     6361 gacaatcagc gtctccgcca gatggctgag ctgctgcaat atgctctctt cagtaaccaa
     6421 agccagcgtt tgcgcccagg cgatgcgcac cataatgcgc cgccggaata gccgcagctc
     6481 gcgcattaac ccggcttcgt cactcacatt actgagcgcc tcctgcaacc atgccgcgta
     6541 atgctgccat tcgtcggcct gcggcggttg gctttccagt tccgtcagcc actctggatg
     6601 cacaatcacg ctgtcctgca caaaatcact aaaagtaagt actgacttcg cctgtgcgct
     6661 aagtgattcc tcggctaaag gctctggcag ccgctcaaca acggtctgcc agtactgctg
     6721 taacggtgaa gagagcggct tcataagcga ttttatcctt gcctgattaa cgttttccgc
     6781 tgtgcaacca gaacggttcc tgattgtttg cctcattacg gaaatgttca atttcaatgc
     6841 gttgcccggt cgcaatagcg tgacgcagcc cctgccagtt ctccagccag gcttgcgcga
     6901 cgacaggatc atagtaacct gccagcagca gtattgagtc aatatcacgc gtcaggcgtg
     6961 gcaactgatc atggtagcga tcgcctaacg gctggcagaa aacgcttttc agttcagcgg
     7021 catggcggga aagatggata tcggcaaagc gtttgaagga atcgctgatt ttaccctgcg
     7081 ctttggcatc taaaaacggc tgccaggctt tgcttaccaa ccattcggtc aacgccagct
     7141 ttgccattgc cgtttcggta gagtagaccg ccgtcacggc agaaaccgca gaagcgatgg
     7201 tcgcttcgca ttgagtcagc aaatcacgta agtgagtgct cgctttacgc ggcacaatgc
     7261 caccgaacag catcagtgca tggcgaacaa ggccaatggc tgccagcacc tgctctttcg
     7321 ccgcatcgtt gccgcgtacc cacagttctt catgatactg ccattgcgct aacgccagct
     7381 cgaacgccgc ttccagcccc tgttccacat cggcttttgc cgcaacatgc aaaatggtgg
     7441 tcggtttgat ttcacgcgcc ggattgccct gcgccagatg atagccacgc gccgctttgc
     7501 tcaggctgcc ctggcgtaat ccggtttgcg ataccagttg gttcgccagt ttcagcaccg
     7561 cgcgcgtgtc gccgctaagc agttccagtt ccagctcaca gataggttca gcaaattccc
     7621 ccgctttcac ctccccctga tcgagagcga tttcaattcg gctgtcatcg accgccacca
     7681 gccatttttc gcgataaaaa tcggtgctga acagcggttg tacgcgggaa gcgagatcgg
     7741 cgggcaattc gccgttcggc cagacttccg tcggtaactg cgccaggtcg agcgtcggtt
     7801 cgctcaacgc cacgttatat tccgggcgct ggtgtaagcc gcctgtcact cttcctgcaa
     7861 ctttcatggt catctcatag cgaccgtttt cgccacgaat acgtaagccc atatcgtgcc
     7921 cacgcagcca gttatccggc gtttcgtagt aaatattcag caactgcacg gggtcatggt
     7981 gctcgccgcc cagcgtattg agatggtcac gcaacgcctc aacggcactg tgattaacaa
     8041 taaactttaa ttcgatttcc tgagccatgg cctttactta tcggttatgt cacataagtg
     8101 acgatgaacg gcgaacttaa tgcgatcttt tttgtcagta gatagtattt tgcgccaaat
     8161 tgccatgcaa cgggcaattt gacgggcgta aaagtttgaa gcagtggcaa gacctgacac
     8221 agaggattcc gattgatgtc gaatgctttg cgtagcgaca ctgatgccac tactatcgtt
     8281 ccactttcca tgacaataac gacagcctga tgccaaaatt acgcctgatc ggattaactt
     8341 tactcgcact tagcgccact gccgtctcac acgctgaaga aacgcgctat gtttctgatg
     8401 aactgaatac ctgggtccgt agcggtccgg gagatcatta tcgcctcgtg ggcacggtta
     8461 acgccggcga ggaagtgacc ttattacaaa ctgacgccaa caccaattat gcccaggtga
     8521 aagacagctc tggccgtacc gcctggatcc cgttgaaaca acttagcact gagccaagcc
     8581 tgcgctcccg tgtgccagat ctggaaaatc aggtcaaaac cctgaccgat aaactcacca
     8641 atatcgataa cacctggaat cagcgcaccg cagaaatgca gcaaaaagtg gcgcagagcg
     8701 acagcgtgat caacggatta aaagaagaaa atcagaaatt gaaaaacgag ctgattgtcg
     8761 cgcagaaaaa ggtcgatgcc gccagcgtac agttggatga caaacagcgc accatcatca
     8821 tgcaatggtt tatgtatggc ggtggcgtgc tggggcttgg cttgctgctc ggtctggtac
     8881 tgccacacct gatcccaacg cgcaaacgca aagatcgctg gatgaactaa atcgccttct
     8941 tcgttgcact gtcttattat taggaattgt gtatttgacg aagggatgat ggcgtgaaga
     9001 tttatctggt cggtggtgct gttcgggatg cattgttagg gctaccggtc aaagacagag
     9061 attgggtggt ggtcggcagt acgccacagg agatgctcga cgcgggctac cagcaggtag
     9121 gccgcgattt tcctgtgttt ctgcatccgc taacgcatga agagtatgcg ctggcacgta
     9181 ccgaacggaa atccggttcc ggttacaccg gttttacttg ctatgccgca ccggatgtca
     9241 cgctggaaga tgatcttaag cgtcgcgatc tgaccattaa tgcgctggct caggacgata
     9301 acggcgagat tatcgacccg tacaacggtc tgggcgatct gcaaaatcgt ctgttgcgcc
     9361 atgtttcccc cgcttttggc gaagatccgt tacgcgttct gcgcgtggcg cgttttgctg
     9421 cgcgttatgc ccacctcggt tttcgtattg ccgatgaaac tctgacgctg atgcgcgaga
     9481 tgacccatgc gggtgaactg gaacacctga cgcctgaacg ggtatggaaa gagacggaaa
     9541 gcgccctcac cacccgcaat ccccaggtgt tcttccaggt actgcgcgat tgcggtgcac
     9601 ttcgcgtttt attcccggaa attgacgccc tgtttggcgt tccggcccct gccaggtggc
     9661 atccggaaat cgacacgggt attcatacct taatgacgct ctcaatggcg gcgatgctga
     9721 gtccgcaggt tgatgtccgt ttcgcaactt tatgccacga tctcggtaaa ggactgacgc
     9781 cgccagaact ttggccgcgt catcatgggc atggcccggc gggtgttaag ttagtggaac
     9841 aactatgcca gcgtctgcgc gtgccaaatg aaattcgcga tttagccaga ctggtggctg
     9901 agtttcacga tctcatccac accttcccaa tgctgaaccc aaaaaccatc gtcaaattat
     9961 ttgattccat cgatgcctgg cgtaaaccgc agcgtgtcga gcaactggcg ctgaccagcg
    10021 aggctgacgt acgcggcaga accggttttg aatcagcgga ctacccgcaa ggccgttggt
    10081 tgcgcgaagc ctgggaagtg gcgcagtcag tgccgacaaa agcggtcgtt gaagcgggat
    10141 ttaaaggtgt ggagattcgc gaggagctga cccgacggcg gattgcggcg gtagccagtt
    10201 ggaaggaaca acgttgccca aagcctgact gagtataaaa ctaaacgtta acacgaacca
    10261 tcccctcgcc cc
//
LOCUS       DAZKHF020000163        10268 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2201S31241
            SAMN43267800-rid25106413.denovo.164, whole genome shotgun sequence.
ACCESSION   DAZKHF020000163 DAZKHF020000000
VERSION     DAZKHF020000163.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267800
            Sequence Read Archive: SRR30307263
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 10268)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10268)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 10268)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350581.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350581.2
            Genome Coverage       :: 151x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:21:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,652
            CDSs (total)                      :: 5,557
            Genes (coding)                    :: 5,320
            CDSs (with protein)               :: 5,320
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 237
            CDSs (without protein)            :: 237
            Pseudo Genes (ambiguous residues) :: 0 of 237
            Pseudo Genes (frameshifted)       :: 102 of 237
            Pseudo Genes (incomplete)         :: 130 of 237
            Pseudo Genes (internal stop)      :: 52 of 237
            Pseudo Genes (multiple problems)  :: 41 of 237
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10268
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267800-rid25106413.denovo.164"
                     /serotype="O 26:H11"
                     /isolate="2201S31241"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-01"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            complement(<1..475)
                     /locus_tag="ACHOSC_RS16435"
                     /old_locus_tag="pgaptmp_003287"
     CDS             complement(<1..475)
                     /locus_tag="ACHOSC_RS16435"
                     /old_locus_tag="pgaptmp_003287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NTP transferase domain-containing protein"
                     /protein_id="HGU4549179.1"
                     /translation="MSAIDCIITAAGLSSRMGQWKMMLPWQQGTILDTSIKNALQFCS
                     RIILVTGYRGNELHERYANQSNITIIHNPDYAQGLLTSVKAAVPAVQTEHCFLTHGDM
                     PTLTIDIFRKIWSLRNDGAILPLHNGIPGHPILVSKPCLMQAIQRPNVTNMRQALL"
     gene            578..1348
                     /gene="yqeC"
                     /locus_tag="ACHOSC_RS16440"
                     /old_locus_tag="pgaptmp_003288"
     CDS             578..1348
                     /gene="yqeC"
                     /locus_tag="ACHOSC_RS16440"
                     /old_locus_tag="pgaptmp_003288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417352.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="selenium cofactor biosynthesis protein YqeC"
                     /protein_id="HGU4549180.1"
                     /translation="MKSIVDPSALVIDLGAQKRPTVISVVGAGGKTSLLFWLAELLQA
                     SGRRVLITTTTHMFMPTSHWPVVFCRDPAMLPHASLTSPISFCFHSWKANQGKVQGFT
                     PEAIDALVQRPECDVILIEADGSRGMPLKAPDEHEPCIPKSSCCVIAVMGGHTLGAKV
                     STENVHRWSQFADITGLTPDATLQLSDLVALVRHPQGAFKNVPQGCRRVWFINRFSQC
                     ENAIAQSELLQPLQQHDVEAIWLGDIQEHPAIARRFVN"
     gene            1396..3021
                     /gene="yqeB"
                     /locus_tag="ACHOSC_RS16445"
                     /old_locus_tag="pgaptmp_003289"
     CDS             1396..3021
                     /gene="yqeB"
                     /locus_tag="ACHOSC_RS16445"
                     /old_locus_tag="pgaptmp_003289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="selenium-dependent molybdenum cofactor
                     biosynthesis protein YqeB"
                     /protein_id="HGU4549181.1"
                     /translation="MNIFTEAAKLEEQNCPFAMAQIVDSRGSTPRHSAQMLVRADGSI
                     VGTIGGGMVERKVIEESLQALQERKPRLFHGRMARNGADAVGSDCGGAMSVFISVHGM
                     RPRLVLIGAGHVNRAIAQSAALLGFDIAVADIYRESLNPELFPPSTTLLHAESFGAAV
                     EALDIRPDNFVLIATNNQDREALDKLIEQPIAWLGLLASRRKVQLFLRQLREKGVAEE
                     HIARLHAPVGYNIGAETPQEIAISVLAEILQVKNNAPGGLMMKPSHPSGHQLVVIRGA
                     GDIASGVALRLYHAGFKVIMLEVEKPTVIRCTVAFAQAVFDGEMTVEGVTARLATSTA
                     EAMKLTERGFIPVMADPACSLLDELKPLCVVDAILAKQNLGTRADMAPVTIALGPGFT
                     AGKDCHAVIETNRGHWLGQVIYSGCAQENTGVPGNIMGHTTRRVIRAPAAGIMRSNVK
                     LGDLVKEGDVIAWIGEHEIKAPLTGMVRGLLNDGLAVVGGFKIGDIDPRGETADFTSV
                     SDKARAIGGGVLEALMMLMHQGVKATKEVLEVA"
     gene            complement(3062..3994)
                     /gene="arcC"
                     /locus_tag="ACHOSC_RS16450"
                     /old_locus_tag="pgaptmp_003290"
     CDS             complement(3062..3994)
                     /gene="arcC"
                     /locus_tag="ACHOSC_RS16450"
                     /old_locus_tag="pgaptmp_003290"
                     /EC_number="2.7.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008804 - carbamate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbamate kinase"
                     /protein_id="HGU4549182.1"
                     /translation="MSKKIVLALGGNALGDDLAGQMKAVKITSQAIVDLIAQGHEVIV
                     THGNGPQVGMINQAFEAAAKTEAHSPMLPMSVCVALSQGYIGYDLQNALREELLSRGI
                     NKPVATLVTQVEVDANDPAFLNPTKPIGSFFTEQEAEQLTKQGYTLKEDAGRGYRRVV
                     ASPKPVDIIEKETVKALVDAGQVVITVGGGGIPVIREGNHLRGASAVIDKDWASARLA
                     EMIDADMLIILTAVEKVAINFGKENEQWLDRLSLSDAERFIEEGHFAKGSMLPKVEAA
                     ASFARSRAGREALITVLSKAKEGIEGKTGTVICQ"
     gene            complement(4042..5427)
                     /gene="hyuA"
                     /locus_tag="ACHOSC_RS16455"
                     /old_locus_tag="pgaptmp_003291"
     CDS             complement(4042..5427)
                     /gene="hyuA"
                     /locus_tag="ACHOSC_RS16455"
                     /old_locus_tag="pgaptmp_003291"
                     /EC_number="3.5.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311773.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-phenylhydantoinase"
                     /protein_id="HGU4549183.1"
                     /translation="MRVLIKNGTVVNADGQAKQDLLIESGIVRQLGNNISPQLPYEEI
                     DATGCYVFPGGVDVHTHFNIDVGIARSCDDFFTGTRAAACGGTTTIIDHMGFGPNGCR
                     LRHQLEVYRGYAAHKAVIDYSFHGVIQHINHAILDEIPMMVEEGLSSFKLYLTYQYKL
                     NDDEVLQALRRLHESGALTTVHPENDAAIASKRAEFIAAGLTAPRYHALSRPLECEAE
                     AIARMINLAQIAGNAPLYIVHLSNGLGLDYLRLARANHQPVWVETCPQYLLLDERSYD
                     TEDGMKFILSPPLRNVREQDKLWCGISDGAIDVVATDHCTFSMAQRLQISKGDFSRCP
                     NGLPGVENRMQLLFSSGVMTGRITPERFVELTSAMPARLFGLWPQKGLLAPGSDGDVV
                     IIDPRQSQQIQHRHLHDNADYSPWEGFTCQGAIVRTLSRGETIFCDGTFTGKAGRGRF
                     LRRKPFVPPVL"
     gene            complement(5480..6691)
                     /gene="ygeY"
                     /locus_tag="ACHOSC_RS16460"
                     /old_locus_tag="pgaptmp_003292"
     CDS             complement(5480..6691)
                     /gene="ygeY"
                     /locus_tag="ACHOSC_RS16460"
                     /old_locus_tag="pgaptmp_003292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YgeY family selenium metabolism-linked
                     hydrolase"
                     /protein_id="HGU4549184.1"
                     /translation="MAKNIPFKLILEKAKDYQADMTRFLRDMVAIPSESCDEKRVVHR
                     IKEEMEKVGFDKVEIDPMGNVLGYIGHGPRLVAMDAHIDTVGIGNIKNWDFDPYEGME
                     TDELIGGRGTSDQEGGMASMVYAGKIIKDLGLEDEYTLLVTGTVQEEDCDGLCWQYII
                     EQSGIRPEFVVSTEPTDCQIYRGQRGRMEIRIDVQGVSCHGSAPERGDNAIFKMGPIL
                     GELQELSQRLGYDEFLGKGTLTVSEIFFTSPSRCAVADSCAVSIDRRLTWGETWEGAL
                     DEIRALPAVQKANAVVSMYNYDRPSWTGLVYPTECYFPTWKVEEDHFTVKALVNAYEG
                     LFGKAPVVDKWTFSTNGVSIMGRHGIPVIGFGPGKEPEAHAPNEKTWKSHLVTCAAMY
                     AAIPLSWLATE"
     gene            complement(6749..7945)
                     /gene="dpaL"
                     /locus_tag="ACHOSC_RS16465"
                     /old_locus_tag="pgaptmp_003293"
     CDS             complement(6749..7945)
                     /gene="dpaL"
                     /locus_tag="ACHOSC_RS16465"
                     /old_locus_tag="pgaptmp_003293"
                     /EC_number="4.3.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008838 - diaminopropionate ammonia-lyase
                     activity [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopropionate ammonia-lyase"
                     /protein_id="HGU4549185.1"
                     /translation="MSVFSLKIDIADNKFFNGETSPLFSQSQAKLARQFHQKIAGYRP
                     TPLCALDDLANLFGVKKILVKDESKRFGLNAFKMLGGAYAIAQLLCEKYHLDIETLSF
                     EHLKNAIGEKMTFATTTDGNHGRGVAWAAQQLGQNAVIYMPKGSAQERVDAILNLGAE
                     CIVTDMNYDDTVRLTMQHAQQHGWEVVQDTAWEGYTKIPTWIMQGYATLADEAVEQMR
                     EMGVTPTHVLLQAGVGAMAGGVLGYLVDVYSPQNLHSIIVEPDKADCIYRSGVKGDIV
                     NVGGDMATIMAGLACGEPNPLGWEILRNCATQFISCQDSVAALGMRVLGNPYGNDPRI
                     ISGESGAVGLGVLAAVHYHPQRQSLMEKLALNKDAVVLVISTEGDTDVKHYREVVWEG
                     KHAVAP"
     gene            complement(8003..9190)
                     /gene="ygeW"
                     /locus_tag="ACHOSC_RS16470"
                     /old_locus_tag="pgaptmp_003294"
     CDS             complement(8003..9190)
                     /gene="ygeW"
                     /locus_tag="ACHOSC_RS16470"
                     /old_locus_tag="pgaptmp_003294"
                     /EC_number="2.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311770.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="knotted carbamoyltransferase YgeW"
                     /protein_id="HGU4549186.1"
                     /translation="MKTVNELIKDINSLTSHLHEKDFLLTWEQTPDELKQVLDVAAAL
                     KALRAENISTKVFNSGLGISVFRDNSTRTRFSYASALNLLGLAQQDLDEGKSQIAHGE
                     TVRETANMISFCADAIGIRDDMYLGAGNAYMREVGAALDDGYKQGVLPQRPALVNLQC
                     DIDHPTQSMADLAWLREHFGSLENLKGKKIAMTWAYSPSYGKPLSVPQGIIGLMTRFG
                     MDVTLAHPEGYDLIPDVVEVAKNNAKASGGSFRQVTSMEEAFKDADIVYPKSWAPYKV
                     MEQRTELLRANDHEGLKALEKQCLAQNAQHKDWHCTEEMMELTRDGEALYMHCLPADI
                     SGVSCKEGEVTEGVFEKYRIATYKEASWKPYIIAAMILSRKYAKPGALLEQLLKEAQE
                     RVK"
     gene            9669..>10268
                     /locus_tag="ACHOSC_RS16475"
                     /old_locus_tag="pgaptmp_003295"
     CDS             9669..>10268
                     /locus_tag="ACHOSC_RS16475"
                     /old_locus_tag="pgaptmp_003295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain-containing protein"
                     /protein_id="HGU4549187.1"
                     /translation="MELATTQSVLMQIQPTIQRFARMLASVLQLEVEIVDENLCRVAG
                     TGAYGKFLGRQLSGNSRLLRHVLETKTEKVVTQSRFDPLCEGCDSKENCREKAFLGTP
                     VILQDRCVGVISLIAVTHEQQEHISDNLREFSDYVRHISTIFVSKLLEDQGPGDNISK
                     IFATMIDNMDQGVLVVDDESRVQFVNQTALKTLGVVQNNI"
BASE COUNT         2426 a         2465 c         2675 g         2702 t
ORIGIN      
        1 taaggagtgc ctgacgcata ttggtgacat tgggtcgttg gattgcctgc atcaagcatg
       61 gttttgacac taaaatcgga tggccgggga tgccattatg gagtggcagt attgcgccat
      121 catttcgtaa cgaccagatt tttctaaaaa tatcgatggt gagggttggc atgtcaccgt
      181 gggtgagaaa acaatgttct gtttgcactg cgggtactgc ggcctttact gatgttagta
      241 aaccctgcgc ataatctggg ttgtggataa tagtgatatt gctctggttc gcataacgtt
      301 cgtgcagttc attaccacga tagccggtga ctaaaataat tcggctacaa aactgcaacg
      361 catttttgat acttgtatca agaattgtcc cctgttgcca gggtaacatc attttccatt
      421 gccccattct tgatgataat ccggcagcgg taattataca gtcgatggct gacatagtgg
      481 ttccattatt tataggggct aataacgcct gataattgcg ttacttcctg atggattaag
      541 ttggggataa tttatcccag agaggtcata aagactcgtg aaaagtatag ttgacccatc
      601 ggcattagtc attgatttag gtgcgcagaa acgtcccact gtgatttctg ttgttggtgc
      661 tggggggaaa accagcttgc ttttttggct ggcagaactg ctccaggcta gcggcaggcg
      721 tgtgttaatc acgacgacga cacatatgtt tatgccaacg tctcactggc ccgtggtttt
      781 ctgtcgtgat cccgccatgc ttcctcatgc gtctcttaca tcccccattt cattttgttt
      841 tcacagctgg aaagcgaacc agggaaaagt gcagggattt acgccagaag cgattgatgc
      901 actggtgcaa cgaccagagt gtgacgtaat tctcattgaa gcagatggct cgcgtggaat
      961 gccgttaaaa gcgcctgatg agcacgaacc ttgcataccc aaaagcagtt gctgcgtgat
     1021 tgctgtgatg ggaggacata ctttgggcgc gaaagtgagc acagaaaatg tccatcgctg
     1081 gtcgcagttt gctgatatta ccgggttaac acctgatgca accttgcaac tgagcgatct
     1141 cgttgcgctg gttcgccacc ctcagggggc gtttaaaaac gtaccgcaag gttgtcggcg
     1201 agtctggttc attaaccgtt tttctcaatg tgagaatgcg attgcacaaa gcgagctcct
     1261 tcaaccgctg caacaacacg acgtagaggc aatctggctg ggcgatatac aagaacatcc
     1321 tgcaatcgcg cgcagatttg tgaattagcg gcccgggtat tccgggttac tgaaaggata
     1381 tccattgagg ttggtatgaa tattttcaca gaggctgcaa aactcgaaga gcaaaattgt
     1441 ccgtttgcga tggcgcaaat tgttgatagc cgaggctcga ctccccgcca ttctgcacaa
     1501 atgttagtgc gcgccgatgg ttctatcgtc ggtacaattg gtggcggaat ggttgagcgg
     1561 aaggtgattg aagagtcact tcaggcattg caggaacgta agccgcgatt attccatgga
     1621 cgtatggctc gtaacggtgc ggatgctgtc gggtcagatt gtggaggtgc aatgtcagta
     1681 tttatcagcg tccatggtat gcgtccacgt ctggtgttga ttggcgcggg gcatgtcaac
     1741 cgggcgatag cccagagtgc ggcgctatta ggatttgata ttgccgttgc cgatatttat
     1801 cgcgaaagcc tcaatcctga actattccct ccatcaacca cgcttctcca tgctgagtcg
     1861 tttggtgcag cagtggaagc actggatatt cgccctgata attttgtcct gattgccacg
     1921 aataatcagg atcgtgaagc cctcgacaaa ctcattgaac agcccattgc atggttgggg
     1981 ttgctggcaa gtcgccgcaa ggttcagctt ttcctgcgtc aattgcgtga gaaaggcgtg
     2041 gctgaagaac atattgcccg tttacatgcg cccgttggtt acaacatagg tgcggaaacg
     2101 ccgcaggaga tcgccatcag cgtgctggca gaaatattac aggtgaaaaa taacgcgccg
     2161 ggtgggctga tgatgaaacc ttctcatcct tccggacacc agctggtggt gattcgcggt
     2221 gcgggagata tcgccagtgg tgtggcgcta cgtctgtatc atgcgggttt taaagtgatc
     2281 atgctggaag tggaaaaacc gacagtgatt cgttgtaccg tggcgtttgc ccaggccgtg
     2341 ttcgatggcg aaatgacggt cgaaggcgtt actgctcgcc tggcaaccag tactgcggaa
     2401 gcgatgaaac ttaccgaacg cggattcatc cctgtgatgg cagatcccgc ctgttcattg
     2461 cttgatgaac tgaaaccgct ttgcgtggtg gacgctattc tggcgaaaca gaatttggga
     2521 acgcgggcag atatggcacc agtaacaatc gcgcttgggc cgggctttac tgcagggaag
     2581 gattgtcatg cggtaattga aacaaatcgc gggcactggc tcggtcaggt gatttactct
     2641 ggttgtgcgc aggagaatac cggtgttcct ggcaatatta tggggcatac cacccgacgg
     2701 gtgatccgtg ctcctgctgc aggcattatg cgatccaacg tgaaattagg cgatctggtg
     2761 aaagagggcg atgtgattgc ctggattggt gagcatgaaa ttaaagcacc gttgacgggg
     2821 atggtgcgtg gcttgttgaa cgacggcctg gcagtggtcg gtggttttaa aattggtgat
     2881 atcgatcctc gtggtgaaac ggctgatttc accagcgttt ctgataaagc ccgggcgatt
     2941 ggcggcggcg tacttgaggc gttaatgatg ttgatgcatc agggcgtgaa agcgacaaaa
     3001 gaagtgctgg aagtggctta aatgaaaatg gccggatggt attacccacc cggccttaaa
     3061 attactgaca aatcaccgtt ccggttttgc cttcaatccc ctctttcgct ttgctcaata
     3121 cggtaatcag tgcttcacga cctgcgcgag agcgagcaaa cgaggcggca gcttccactt
     3181 taggcagcat agaacctttc gcaaaatgac cttcttcgat aaagcgttca gcatcactta
     3241 atgacaggcg atcgagccac tgttcattct ctttaccgaa gttaatggcc actttttcta
     3301 ccgcagtcag aatgatcagc atatcggcat caatcatttc tgctaaacgg gcgctggccc
     3361 agtctttatc gataaccgcg ctggcaccac gcagatggtt accttcacga ataacaggaa
     3421 taccaccacc gccaacggtg atcaccacct ggcctgcatc taccagagct ttaaccgttt
     3481 ctttttcaat aatatcaacc ggttttggcg aggcaacaac acggcgatag ccgcgaccag
     3541 catcttcttt cagagtgtaa ccctgttttg tcagttgctc tgcttcttgc tcggtaaaga
     3601 acgaaccgat cggcttggtc gggttgagga atgccggatc gttagcgtcg acttcgacct
     3661 gagtaaccag cgttgctacc ggtttattaa tgccccgaga aagcagttct tctcttaagg
     3721 cgttttgtag atcgtaacca atataaccct ggcttaaggc aacgcagaca gacatcggca
     3781 gcattggcga gtgcgcttca gtttttgcag cagcttcaaa ggcctggtta atcatgccca
     3841 cctgcggccc gttaccatga gtgacgataa cttcatgtcc ttgggcaatt aaatcaacaa
     3901 ttgcctgaga agtaattttt accgctttca tttgtccggc caggtcatcg cccagcgcat
     3961 tcccgcccag ggcgagaaca attttcttac tcatttttcc tcactcatca ttcttgattg
     4021 tgtatagatc cctgacagtg gttagagcac gggagggaca aacggtttgc gtcgcaggaa
     4081 acgacctcgc ccggctttgc ctgtaaaggt gccgtcacag aaaatcgttt caccacggga
     4141 taaggttctg acaatcgcgc cctgacaggt aaaaccctcc catggcgagt agtcggcgtt
     4201 gtcgtggaga tggcgatgct gaatttgttg gctctgacgt gggtcgataa tcaccacgtc
     4261 gccatcggaa ccgggcgcta ataatccttt ttgcggccac aggccaaaca gcctggcggg
     4321 cattgcgctg gttaattcaa caaagcgttc cggtgttata cgtcccgtca tcacgccact
     4381 ggaaaacagt aactgcatgc ggttttccac accgggtaag ccatttgggc agcgactgaa
     4441 atcgccttta gaaatttgca ggcgttgagc catcgagaag gtgcagtgat cggtcgccac
     4501 cacgtcaatc gcaccatcgc tgatgccaca ccacagtttg tcctgctcgc gtacgttacg
     4561 cagcggtggg ctaagaatga acttcatgcc gtcttctgta tcgtaactgc gttcgtccaa
     4621 caggagatat tgtgggcagg tttcaaccca gactggctgg tgattcgcac gggcaagacg
     4681 cagataatcc agacctaagc cgttagacag gtgcacgata tagagcgggg cgttaccggc
     4741 aatttgtgcc aggttaatca tgcgggcgat ggcttccgct tcgcattcca gagggcgact
     4801 caaggcatga tagcgcggcg cggttaaccc tgcggcgata aattccgccc gcttgctggc
     4861 gatagccgca tcattttccg ggtgcacggt ggtcagcgcg ccggattcat gcagacggcg
     4921 taatgcctgc aaaacctcgt catcgttgag tttgtattga taggttaaat agagtttaaa
     4981 actgctcagt ccttcctcga ccatcatcgg gatttcgtcg aggattgcgt gattaatgtg
     5041 ctggatcaca ccgtgaaagc tgtaatcgat gaccgcttta tgggcggcat aaccacgata
     5101 aacctccagt tgatggcgta accgacagcc gtttgggcca aatcccatat ggtcaataat
     5161 ggttgttgta ccgccacacg cagctgcgcg ggtaccggta aaaaaatcat cacaactgcg
     5221 cgcgatgccg acatcaatat tgaaatgcgt atggacatcc acgccgccag ggaaaacgta
     5281 acagccagtg gcatcaattt cttcatacgg gagctgcggc gaaatattgt tgcccaactg
     5341 gcgaacaatc ccgctttcaa tcagcaaatc ctgtttggct tgtccatctg cgttaacgac
     5401 agtgccgttt ttgatcaata cgcgcatagc aaactccaaa aaccctccgg cgaaccggag
     5461 gggaataagt gaagagtaat tactctgttg ccagccagct taacgggatt gcagcgtaca
     5521 tcgcggcaca ggtcaccagg tgagatttcc aggttttttc gttaggtgca tgcgcttcag
     5581 gttctttacc cgggccaaag ccgatcaccg gaatgccgtg acggcccatg atagatacgc
     5641 cgttagttga gaaggtccac ttatcaacaa ccggcgcttt gccaaacagc ccttcgtagg
     5701 cattcaccag tgctttaacg gtgaagtgat cttcttccac tttccaggtc gggaagtagc
     5761 attcggttgg gtaaaccagg ccagtccagg acggacggtc gtagttgtac atagaaacaa
     5821 ccgcgttagc tttctgtact gcaggcaggg cgcggatttc gtccagcgcg ccttcccagg
     5881 tttcgcccca ggtcagacgg cggtcaatag agacggcgca gctatctgct acagcgcaac
     5941 ggcttgggga tgtgaagaag atttcagaaa cggtgagggt gcctttgccg aggaattcgt
     6001 cataacccag acgttgggag agttcttgta attcgccaag aatcggaccc attttgaaaa
     6061 tggcgttgtc accgcgttct ggcgcagaac cgtggcagct aacaccctga acatcaatac
     6121 gaatttccat acgaccgcgc tgaccacggt atatctggca gtcggttggt tcggtactga
     6181 ccacaaattc cgggcgaatg ccggattgtt caataatgta ctgccagcac agaccgtcgc
     6241 agtcttcttc ctgcacagta ccggtaacca gcagggtata ttcatcttcc agaccgaggt
     6301 ctttaatgat tttaccggca taaaccatag atgccatgcc gccttcctgg tcggaagtac
     6361 cgcgaccacc gattagctca tcagtttcca tgccttcgta cggatcgaag tcccagtttt
     6421 tgatgttgcc aatgccgacg gtatcgatat gagcgtccat tgccaccaga cgcgggccgt
     6481 ggccgatata accgagaacg ttgcccatcg ggtcgatttc aactttatcg aagccgactt
     6541 tttccatctc ttctttaata cgatgtacta cgcgtttttc gtcgcagctt tcactgggaa
     6601 tagcaaccat gtcgcgcagg aagcgagtca tgtccgcctg gtaatctttt gctttttcaa
     6661 gaatcagttt gaatggaata ttcttagcca ttatttttct ccagattttt tactccccgt
     6721 tttgcaggga gtagttccaa tagagtgatt aaggtgctac agcgtgtttg ccttcccaga
     6781 caacttcgcg gtagtgcttc acgtcggtgt cgccttcagt gctgataacc agcaccacgg
     6841 catctttgtt cagcgccagt ttttccatca ggctttgacg ttgcgggtga taatgaaccg
     6901 ctgcgagaac gcccaaaccg acagcgccgg attcaccgga gatgatgcgc gggtcgttgc
     6961 cgtacggatt acccagcacg cgcataccta atgcggcaac gctgtcctgg caggagatga
     7021 attgggtggc gcagttacgt aggatttccc agcccagcgg gttaggttcg ccacaggcca
     7081 ggcctgccat gatggtggcc atatcaccgc caacgttgac gatgtcgcct ttgacgccgg
     7141 agcgataaat acagtcagct ttgtcaggtt caacaataat gctgtgcaga ttttgcgggc
     7201 tatagacgtc gaccagataa cccagcacac caccggccat tgctccgaca ccggcttgca
     7261 gcagaacgtg cgtcggggtt acgcccattt cacgcatttg ctcgacggct tcatctgcca
     7321 gggttgcgta gccttgcatg atccaggttg ggattttggt gtaaccttcc catgccgtgt
     7381 cctgtaccac ttcccagccg tgctgctgcg cgtgttgcat ggtcaggcga acggtatcgt
     7441 catagttcat atctgtgacg atgcactcgg caccgaggtt cagaatggcg tcaacgcgtt
     7501 cctgagcaga acctttcggc atgtaaatca ccgcattctg tccgagttgc tgcgctgccc
     7561 acgccacacc gcgcccgtgg ttgccgtcgg tggtggtcgc gaaagtcatt ttttcgccga
     7621 tggcattttt caggtgctca aatgacagcg tttcgatatc aagatgatat ttttcacaca
     7681 ataattgagc gatagcgtac gcaccgccga gcattttgaa ggcgttcaga ccgaatcgtt
     7741 ttgattcgtc tttgacaaga attttcttca ccccaaaaag gtttgcgaga tcgtccagcg
     7801 cacaaagcgg tgttgggcga taaccagcta ttttctggtg gaactggcgc gccagtttgg
     7861 cctggctttg cgaaaagagc ggtgatgttt cgccgttgaa aaatttgtta tcggcgatat
     7921 caatcttcaa tgagaaaacg gacatatcct atccttttgg cctctggaaa tagagggccg
     7981 accagctgat cggcccgagg ggttatttca cgcgttcttg cgcttctttc agcagttgct
     8041 cgagcagtgc acctggtttg gcgtatttac gggacaggat catcgcggcg atgatataag
     8101 gcttccagct ggcttctttg taggtagcga tacggtattt ttcgaatacg ccttcagtca
     8161 cttccccttc tttgcaggat acgccgctga tatcagctgg caggcagtgc atgtacaggg
     8221 cttcgccatc acgggtcagt tccatcatct cttcagtaca atgccagtct ttgtgttgcg
     8281 cgttctgtgc cagacactgt ttttccagtg cttttaagcc ttcgtgatcg ttagcgcgca
     8341 gcaattcggt acgctgttcc atcactttgt aaggtgccca tgacttcgga taaacgatgt
     8401 ctgcatcttt gaacgcttct tccatgctgg tgacctgacg gaagctaccg ccagatgctt
     8461 tagcattgtt tttcgccact tcaaccacat ccgggatcag gtcgtagcct tccggatggg
     8521 ccagggtgac atccataccg aagcgagtca tcagaccgat gatgccttgt ggtacagaga
     8581 gcggtttgcc atagcttgga gagtaggccc aggtcatggc gattttttta cctttcaggt
     8641 tttccagtga accaaagtgt tcacgtaacc acgcgaggtc agccattgac tgagtcgggt
     8701 ggtcaatatc gcattgcagg ttcactaaag ccggacgctg tggcagtaca ccctgcttgt
     8761 aaccgtcatc aagtgcagcg ccaacttcac gcatataggc gttgcctgcg cccagataca
     8821 tatcgtcgcg aataccaata gcgtcggcgc agaaggagat catattggcg gtttcacgca
     8881 cggtttcgcc gtgagcgatt tgtgatttgc cttcgtcgag atcttgttgt gcaaggccga
     8941 gcaggttaag cgcggaagca taagagaagc gggtacgggt ggagttgtcg cggaatacgg
     9001 aaatacctaa tccactatta aagactttgg ttgagatgtt ttcagcacgc agtgctttta
     9061 atgctgcggc aacgtccagt acttgtttca gttcatctgg cgtctgttcc cacgttaaca
     9121 aaaaatcttt ctcgtgaagg tgagaggtca gcgaattgat atccttaatc agctcattaa
     9181 cagttttcat cataaagtcc cggtagtgga atgacaggca aagtggttta tttcactgtg
     9241 acttgccaaa gtgattggtg ttttcacagc tatacctggg actaacaata agtgtgccag
     9301 aattatccac ggggctgcaa aagactaatt tgacggataa tgtgtggggt tgatcacaaa
     9361 ttgatgccag cttccggttg tttgtaaaat acgaaaacgt ttggcttttt ttatcgcgtt
     9421 atttatttgc tgctaagtat cagaaataag ataaagggac aggctatctc tcttcagttt
     9481 gataaactca ccgccgtaat tctcatattg atatagaaat gtgatatctc ttccataatt
     9541 tcattagaaa atgataaaaa tcttatgttt ttatcaaagc caatttcgcc tgagttaata
     9601 tcatcatgat ctgtgaacca tcaacgtctt caatgatcga cttttaagta agtctggaag
     9661 gtaaagctat ggagcttgct actacgcagt cagtattgat gcaaattcaa ccgacaattc
     9721 agcgttttgc cagaatgctt gccagcgttt tgcagcttga ggttgagatc gttgatgaaa
     9781 acttgtgtcg cgtcgccgga acgggcgcgt atgggaagtt tcttggtcgc cagttgagcg
     9841 gcaactcacg cctgctccgc cacgtcctgg aaacgaaaac tgaaaaagtt gtgacacagt
     9901 ctcgcttcga tcccctttgc gaaggttgcg atagtaaaga aaattgccgt gaaaaagcat
     9961 ttctgggtac gcctgtcatt ttacaggatc gttgtgttgg ggtgataagt ttgattgccg
    10021 ttacccacga gcaacaagag catatcagtg ataatttacg cgaattttct gattatgttc
    10081 gccatatatc caccattttt gtttcgaaac ttctggagga tcaggggcca ggagataaca
    10141 tcagtaaaat attcgcgacc atgatcgata atatggatca gggcgtatta gttgttgatg
    10201 atgaaagtcg ggttcagttt gttaatcaga ctgccttaaa aacacttggt gttgtacaaa
    10261 ataatatt
//
LOCUS       DAZKHF020000164        10202 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2201S31241
            SAMN43267800-rid25106413.denovo.165, whole genome shotgun sequence.
ACCESSION   DAZKHF020000164 DAZKHF020000000
VERSION     DAZKHF020000164.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267800
            Sequence Read Archive: SRR30307263
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 10202)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10202)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 10202)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350581.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350581.2
            Genome Coverage       :: 151x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:21:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,652
            CDSs (total)                      :: 5,557
            Genes (coding)                    :: 5,320
            CDSs (with protein)               :: 5,320
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 237
            CDSs (without protein)            :: 237
            Pseudo Genes (ambiguous residues) :: 0 of 237
            Pseudo Genes (frameshifted)       :: 102 of 237
            Pseudo Genes (incomplete)         :: 130 of 237
            Pseudo Genes (internal stop)      :: 52 of 237
            Pseudo Genes (multiple problems)  :: 41 of 237
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10202
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267800-rid25106413.denovo.165"
                     /serotype="O 26:H11"
                     /isolate="2201S31241"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-01"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            <1..1189
                     /locus_tag="ACHOSC_RS16480"
                     /old_locus_tag="pgaptmp_003296"
     CDS             <1..1189
                     /locus_tag="ACHOSC_RS16480"
                     /old_locus_tag="pgaptmp_003296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="APC family permease"
                     /protein_id="HGU4549188.1"
                     /translation="GQAALLMEASTWVAFAFGGIVAMFSGYAYARLGASYPSNGGIID
                     FFRRGLGNGVFSLALSLLYLLTLAVSIAMVARAFGAYAVQFLHEGSQEEHLILLYALG
                     IIAVMTLFNSLSNHAVGRLEVILVGIKMMILLLLIIAGVWSLQPAHISVSAPPSSGAF
                     FSCIGITFLAYAGFGMMANAADKVKDPQVIMPRAFLVAIGVTTLLYISLALVLLSDVS
                     ALELEKYADTAVAQAASPLLGHVGYVIVVIGALLATASAINANLFAVFNIMDNMGSER
                     ELPKLMNKSLWRQSTWGNIIVVVLIMLMTAALNLGSLASVASATFLICYLAVFVVAIR
                     LRHDIHASLPILIVGTLVMLLVIVGFIYSLWSQGSRALIWIIGSLLLSLIVAMVMKRN
                     KTV"
     gene            1220..1317
                     /locus_tag="ACHOSC_RS16485"
                     /old_locus_tag="pgaptmp_003297"
                     /pseudo
     CDS             1220..1317
                     /locus_tag="ACHOSC_RS16485"
                     /old_locus_tag="pgaptmp_003297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308571.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            1314..1721
                     /gene="cueR"
                     /locus_tag="ACHOSC_RS16490"
                     /old_locus_tag="pgaptmp_003298"
     CDS             1314..1721
                     /gene="cueR"
                     /locus_tag="ACHOSC_RS16490"
                     /old_locus_tag="pgaptmp_003298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cu(I)-responsive transcriptional regulator"
                     /protein_id="HGU4549189.1"
                     /translation="MNISDVAKITGLTSKAIRFYEEKGLVTPPMRSENGYRTYTQQHL
                     NELTLLRQARQVGFNLEESGELVNLFNDPQRHSADVKRRTLEKVAEIERHIEELQSMR
                     DQLLALANACPGDDSADCPIIENLSGCCHHRAG"
     gene            complement(1722..2180)
                     /gene="ybbJ"
                     /locus_tag="ACHOSC_RS16495"
                     /old_locus_tag="pgaptmp_003299"
     CDS             complement(1722..2180)
                     /gene="ybbJ"
                     /locus_tag="ACHOSC_RS16495"
                     /old_locus_tag="pgaptmp_003299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415021.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NfeD family protein"
                     /protein_id="HGU4549190.1"
                     /translation="MMELMVVHPHIFWLSLGGLLLAAEMLGGNGYLLWSGVAAVITSL
                     VVWLVPLGWEWQGVMFAILTLLAAWLWWKWLSRRVREQKHSDSHLNQRGQQLIGRRFV
                     LESPLVNGRGHMRVGDSSWPVSASEDLGAGTHVEVIAIEGITLHIRAVSS"
     gene            complement(2177..3094)
                     /gene="qmcA"
                     /locus_tag="ACHOSC_RS16500"
                     /old_locus_tag="pgaptmp_003300"
     CDS             complement(2177..3094)
                     /gene="qmcA"
                     /locus_tag="ACHOSC_RS16500"
                     /old_locus_tag="pgaptmp_003300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH domain-containing protein"
                     /protein_id="HGU4549191.1"
                     /translation="MLIFIPILIFVALVIVGAGVKIVPQGYQWTVERFGRYTKTLQPG
                     LSLVVPFMDRIGRKINMMEQVLDIPSQEVISKDNANVTIDAVCFIQVIDAPRAAYEVS
                     NLELAIINLTMTNIRTVLGSMELDEMLSQRDSINSRLLRIVDEATNPWGIKVTRIEIR
                     DVRPPAELISSMNAQMKAERTKRAYILEAEGIRQAEILKAEGEKQSQILKAEGERQSA
                     FLQAEARERSAEAEARATKMVSEAIASGDIQAVNYFVAQKYTEALQQIGSSSNSKVVM
                     MPLEASSLMGSIAGIAELVKDSANKRTQP"
     gene            3240..3917
                     /gene="fetA"
                     /locus_tag="ACHOSC_RS16505"
                     /old_locus_tag="pgaptmp_003301"
     CDS             3240..3917
                     /gene="fetA"
                     /locus_tag="ACHOSC_RS16505"
                     /old_locus_tag="pgaptmp_003301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter ATP-binding protein FetA"
                     /protein_id="HGU4549192.1"
                     /translation="MQENSPLLQLQNVGYLAGDAKILNNINFSLRAGEFKLITGPSGC
                     GKSTLLKIVASLISPTNGTLLFEGEDVSTLKPEIYRQQVSYCAQTPTLFGDTVYDNLI
                     FPWQIRNQQPDPAIFLDFLERFALPDSILTKNIAELSGGEKQRISLIRNLQFMPKVLL
                     LDEITSALDESNKHNVNEMIHRYVREQNIAVLWVTHDKDEINHADKVITLQPHAGEMQ
                     EARYELA"
     gene            3904..4683
                     /gene="fetB"
                     /locus_tag="ACHOSC_RS16510"
                     /old_locus_tag="pgaptmp_003302"
     CDS             3904..4683
                     /gene="fetB"
                     /locus_tag="ACHOSC_RS16510"
                     /old_locus_tag="pgaptmp_003302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308581.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron export ABC transporter permease subunit
                     FetB"
                     /protein_id="HGU4549193.1"
                     /translation="MNSHNITNESLALALMLVVVAILISHKEKLALEKDIFWSVGRAI
                     IQLIIVGYVLKYIFSVDDASLTLLMVLFICFNAAWNAQKRSKYIAKAFISSFIAITVG
                     AGITLAVLILSGSIEFIPMQVIPIAGMIAGNAMVAVGLCYNNLGQRVISEQQQIQEKL
                     SLGATPKQASAILIRDSIRAALIPTVDSAKTVGLVSLPGMMSGLIFAGIDPVKAIKYQ
                     IMVTFMLLSTASLSTIIACYLTYRKFYNSRHQLVVTQLKKK"
     gene            complement(4746..5600)
                     /gene="cnoX"
                     /locus_tag="ACHOSC_RS16515"
                     /old_locus_tag="pgaptmp_003303"
     CDS             complement(4746..5600)
                     /gene="cnoX"
                     /locus_tag="ACHOSC_RS16515"
                     /old_locus_tag="pgaptmp_003303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308582.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chaperedoxin"
                     /protein_id="HGU4549194.1"
                     /translation="MSVENIVNINESNLQQVLEQSMTTPVLFYFWSERSQHCLQLTPI
                     LESLAAQYNGQFILAKLDCDAEQMIAAQFGLRAIPTVYLFQNGQPVDGFQGPQPEEAI
                     RALLDKVLPREEELKAQQAMQLMQEGNYTDALPLLKDAWQLSNQNGEIGLLLAETLIA
                     LNRSEDAETVLKTIPLQDQDTRYQGLVAQIELLKQAADTPEIQQLQQQVAENPEDAAL
                     ATQLALQLHQVGRNEEALELLFGHLRKDLTAADGQTRKTFQEILAALGTGDALASKYR
                     RQLYALLY"
     gene            complement(5661..6470)
                     /gene="ybbO"
                     /locus_tag="ACHOSC_RS16520"
                     /old_locus_tag="pgaptmp_003304"
     CDS             complement(5661..6470)
                     /gene="ybbO"
                     /locus_tag="ACHOSC_RS16520"
                     /old_locus_tag="pgaptmp_003304"
                     /EC_number="1.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP(+)-dependent aldehyde reductase"
                     /protein_id="HGU4549195.1"
                     /translation="MTHKATEILTGKVMQKSVLITGCSSGIGLESALELKRQGFHVLA
                     GCRKPDDVERMNNMGFTGVLIDLDSPESVDRAADEVIALTDNCLYGIFNNAGFGMYGP
                     LSTISRAQMEQQFSANFFGAHQLTMRLLPAMLPHGEGRIVMTSSVMGLISTPGRGAYA
                     ASKYALEAWSDALRMELRHSGIKVSLIEPGPIRTRFTDNVNQTQSDKPVENPGIAARF
                     TLGPEAVVDKVRHAFISEKPKMRYPVTLVTWAVMVLKRLLPGRVMDKILQG"
     gene            complement(6460..7083)
                     /gene="tesA"
                     /locus_tag="ACHOSC_RS16525"
                     /old_locus_tag="pgaptmp_003305"
     CDS             complement(6460..7083)
                     /gene="tesA"
                     /locus_tag="ACHOSC_RS16525"
                     /old_locus_tag="pgaptmp_003305"
                     /EC_number="3.1.1.5"
                     /EC_number="3.1.2.14"
                     /EC_number="3.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005225520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016298 - lipase activity [Evidence IEA];
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multifunctional acyl-CoA thioesterase I/protease
                     I/lysophospholipase L1"
                     /protein_id="HGU4549196.1"
                     /translation="MNFNNVFRWHLPFLFLVLLTFRAAAADTLLILGDSLSAGYRMSA
                     SAAWPALLNDKWQSKTSVVNASISGDTSQQGLARLPALLKQHQPRWVLVELGGNDGLR
                     GFQPQQTEQTLRQILQDVKAANAEPLLMQIRLPANYGRRYNEAFSAIYPKLAKEFDVP
                     LLPFFMEEVYLKPQWMQDDGIHPNRDAQPFIADWMAKQLQPLVNHDS"
     gene            7054..7740
                     /gene="ybbA"
                     /locus_tag="ACHOSC_RS16530"
                     /old_locus_tag="pgaptmp_003306"
     CDS             7054..7740
                     /gene="ybbA"
                     /locus_tag="ACHOSC_RS16530"
                     /old_locus_tag="pgaptmp_003306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative ABC transporter ATP-binding protein
                     YbbA"
                     /protein_id="HGU4549197.1"
                     /translation="MPAENIVEVHHLKKSVGQGEHELSILTGVELVVKRGETIALVGE
                     SGSGKSTLLAILAGLDDGSSGEVSLVGQPLHNMDEEARAKLRAKHVGFVFQSFMLIPT
                     LNALENVELPALLRGESSAESRNGAKALLEQLGLGKRLDHLPAQLSGGEQQRVALARA
                     FNGRPDVLFADEPTGNLDRQTGDKIADLLFSLNREHGTTLIMVTHDLQLAARCDRCLR
                     LVNGQLQEEA"
     gene            7737..10151
                     /gene="ybbP"
                     /locus_tag="ACHOSC_RS16535"
                     /old_locus_tag="pgaptmp_003307"
     CDS             7737..10151
                     /gene="ybbP"
                     /locus_tag="ACHOSC_RS16535"
                     /old_locus_tag="pgaptmp_003307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative ABC transporter permease subunit YbbP"
                     /protein_id="HGU4549198.1"
                     /translation="MIARWFWREWRSPSLLIVWLALSLAVACVLALGNISDRMEKGLS
                     QQSREFMAGDRALRSSREVPQAWLEEAQKRGLKVGKQLTFATMTFAGDTPQLANVKAV
                     DDIYPMYGDLQTNPPGLKPQAGSVLLAPRLMALLNLKTGDTIDVGDATLRIAGEVIQE
                     PDSGFNPFQMAPRLMMNLADVDKTGAVQPGSRVTWRYKFGGNENQLDGYEKWLLPQLK
                     PEQRWYGLEQDEGALGRSMERSQQFLLLSALLTLLLAVAAVAVAMNHYCRSRYDLVAI
                     LKTLGAGRAQLRKLIVGQWLMVLALSAVTGGAIGLLFENVLMVLLKPVLPAALPPASL
                     WPWLWALGTMTVISLLVGLRPYRLLLATQPLRVLRNDVVANVWPLKFYLPIVSVVVVL
                     LLAGLMGGSMLLWAVLAGAVVLALLCGVLGWMLLNVLRRMTLKSLPLRLAVSRLLRQP
                     WSTLSQLSAFSLSFMLLALLLVLRGDLLDRWQQQLPPESPNYFLINIATEQVEPLKAF
                     LAEHQIVPESFYPVVRARLTAINDKPTEGNEDEALNRELNLTWQNTRPDHNPIVAGNW
                     PPKADEVSMEEGLAKRLNVALGDTVTFMGDTQEFRAKVTSLRKVDWESLRPNFYFIFP
                     EGALDGQPQSWLTSFRWENGNGMLTQLNRQFPTISLLDIGAILKQVGQVLEQVSRALE
                     VMVVLVTACGMLLLLAQVQVGMRQRHQELVVWRTLGAGKKLLRTTLWCEFAMLGFVSG
                     LVAAIGAETALAVLQAKVFDFPWEPDWRLWIVLPCSGALLLSLFGGWLGARLVKGKAL
                     FRQFAG"
BASE COUNT         2235 a         2631 c         2856 g         2480 t
ORIGIN      
        1 ggggcaggct gcattgctaa tggaagcctc gacctgggtc gcctttgctt ttggcggtat
       61 tgtggcgatg ttttccggtt atgcctatgc gcgtctgggg gcgagctatc ccagcaatgg
      121 cggcattatc gacttctttc gtcgcggatt aggcaacggc gtcttttcgc tggcgctctc
      181 gttactgtac ctgttgacgc tggcggtgag catcgccatg gtcgcccgtg cttttggcgc
      241 ttatgccgtg cagtttttgc atgaaggcag ccaggaggag caccttattt tgctctacgc
      301 gttggggatc attgcggtga tgacgctttt caactcctta agcaaccatg cggtagggcg
      361 gctggaagtg atcctcgtcg gcattaaaat gatgatcctg ttattgctga ttattgccgg
      421 tgtctggtcg ctgcaaccgg cgcatatttc cgtctctgcg ccccccagct ccggtgcgtt
      481 cttctcctgt atcgggataa ccttccttgc ctatgcgggc tttggcatga tggcgaacgc
      541 ggcggataaa gtgaaagatc cgcaggtcat tatgccgcgg gcgtttctgg tggcgattgg
      601 cgttaccacg ttgctttata tctcgctggc actggttttg cttagcgatg tatcggcatt
      661 agagttagaa aaatatgccg ataccgccgt agcgcaggct gcttctccgc tgctcggaca
      721 tgtgggttat gtgatcgtcg tcatcggcgc tttactggcg acggcttcag ccattaacgc
      781 gaacctgttc gccgtgttta acatcatgga caacatgggc agcgaacgcg aactgccgaa
      841 gctaatgaat aaatccctgt ggcggcagag tacctggggc aacatcattg tcgtggtgtt
      901 gattatgctg atgacggcgg cactaaattt aggctcactc gccagcgttg ccagcgccac
      961 ctttttgatt tgctatctgg cggtgtttgt ggtggcgatc cgcctgcgtc atgatattca
     1021 cgcctcgttg ccgattctta tcgttggtac gttggtgatg ttgttggtga tcgttggctt
     1081 tatctacagt ctgtggtccc agggtagccg tgcgttgata tggattattg gctcactctt
     1141 actcagcctt attgtggcaa tggtcatgaa gcgcaataaa accgtataac atctctctgt
     1201 gcgcagtact tcctgtatta ttgtggtggc ggtcgatatt cgcactggca aaaaaacgtg
     1261 cttgaatatc tgttgaaacc ctttaacaaa gcacaggagg cgttgcgcga acgatgaaca
     1321 tcagcgatgt agcaaaaatt accggcctga ccagcaaagc cattcgcttc tatgaagaga
     1381 aggggctggt gacgccgccg atgcgcagcg aaaacggtta tcgcacctac acgcagcagc
     1441 atctcaacga actgacctta ctgcgccagg cacggcaggt gggctttaac ctggaagaga
     1501 gcggcgagct ggtgaatctg tttaacgacc cgcagcggca cagcgccgac gtcaaacggc
     1561 gcacgctgga gaaggtggcg gagatcgaac gacacattga ggagctgcaa tccatgcgcg
     1621 accagctgct ggcactggcg aatgcctgcc ctggcgatga cagcgccgac tgcccgatta
     1681 tcgaaaatct ctccggctgc tgtcatcatc gggcagggtg attaagacga gacggctcgg
     1741 atatgtaggg ttatcccttc tatcgcaatg acttcaacat gcgtacctgc accgagatcc
     1801 tcgctggcgc tgacaggcca tgaactgtca ccgacgcgca tatgaccgcg cccgttgacc
     1861 agcggagatt ccagcacaaa acgtcggcca atcagctgct gcccgcgctg gtttaaatga
     1921 ctatcgctgt gcttttgttc gcgcacccgc cgcgacaacc atttccacca cagccaggcg
     1981 gcgagcagcg tcaggatggc aaacattacc ccttgccact cccaacccag cggcaccagc
     2041 cagaccacca ggctggtaat caccgctgcc acgccgctcc acaacaaata accatttccg
     2101 cccagcatct cggctgccag cagcaaaccg ccgagactga gccagaaaat atgtggatga
     2161 acgaccatta actccatcat ggctgagtcc gtttgttggc gctgtctttc accagctcgg
     2221 caatcccggc aatcgacccc atcaggctgc tggcctctaa tggcatcatc actactttgc
     2281 tgttactgga ggaaccgatc tgctgtaacg cttcggtgta tttctgcgct acgaagtagt
     2341 tcaccgcctg aatatcaccg gaggcgatag cttcagacac cattttggtg gcgcgggctt
     2401 ctgcttcggc ggaacgttca cgcgcttcag cctgtaaaaa cgccgactga cgttcgcctt
     2461 ccgctttcag gatttgcgac tgtttttcac cttcggcttt gaggatttcc gcctgacgga
     2521 tcccttccgc ttcaagaatg taagcgcgtt tggtacgttc cgctttcatc tgcgcgttca
     2581 ttgaagagat aagctctgcc ggtgggcgca cgtcgcgaat ttcaatacgg gtgactttaa
     2641 tcccccacgg gttggtggct tcatcgacaa tacgcagcag gcgtgagttg atgctgtcgc
     2701 gctgagagag catttcgtca agttccattg aacccaacac ggtacggatg ttagtcatgg
     2761 tcaggttgat gatcgccagc tccagattgc tgacttcata agccgcgcgc ggcgcgtcaa
     2821 tcacctgaat aaaacagact gcgtcgatgg taacgttggc gttatctttc gagataactt
     2881 cctgggaagg gatatcgagc acttgctcca tcatattgat cttgcgacca atgcgatcca
     2941 taaacggcac caccagactg agccccggct gtaacgtttt ggtatagcga ccaaaacgtt
     3001 ctaccgtcca ttgatagccc tgcggcacga ttttgacgcc cgcgccgaca atgaccagcg
     3061 cgacaaaaat gagaatcggg ataaagataa gcatcggaaa aacctcctgt tgtaccgtcc
     3121 ataatcagca aaattgctgc ttgattaaac aaattatacc tgattactga aagagagttc
     3181 ccccttattc ctgcgaagga taaactgttt ttagtaaaaa tcagaaaaag ggaacagtga
     3241 tgcaggaaaa tagtccttta cttcagctac aaaacgtagg atatctggcg ggtgatgcga
     3301 agattcttaa taacatcaat ttttcgctgc gtgctggcga atttaagtta attaccggtc
     3361 cttccggttg tggcaaaagt acgctgctaa aaatagttgc ttcattgatc agcccaacca
     3421 acggaacgtt actgtttgaa ggtgaggatg tcagcacact aaagccagaa atctaccgcc
     3481 agcaagtctc ttactgcgcc cagacgccga cgctgtttgg cgatacggta tacgataatc
     3541 tgatctttcc ctggcagatc cgtaaccagc agcctgaccc ggccattttt ctcgattttc
     3601 tcgaacgctt cgctttgccg gacagcattt tgacgaaaaa tatcgccgag ctatctggtg
     3661 gtgaaaaaca acgcatctca ctgattcgta acctgcaatt tatgccgaag gttttattgc
     3721 tggatgaaat caccagtgcg ctggatgaaa gtaataaaca taacgttaat gagatgatcc
     3781 atcgttatgt gcgcgagcaa aatattgccg tgctgtgggt gacacacgat aaagacgaaa
     3841 ttaatcatgc ggataaagtg attacactgc aaccgcatgc cggagaaatg caggaagcac
     3901 gctatgaact cgcataatat tactaacgaa tcattggcac tggcattaat gctggtggtg
     3961 gtggcaatct taattagcca taaagaaaaa ctggcgctgg agaaagatat tttctggagc
     4021 gtcgggcgag cgataattca gctgattatt gtcggctatg tgctgaagta tattttcagc
     4081 gtggatgatg ccagcctgac attattgatg gtgttattta tctgctttaa tgcggcgtgg
     4141 aacgcgcaaa aacgcagtaa atatattgct aaagctttta tctcatcgtt tattgccatt
     4201 acggtcgggg cgggaattac cctggcggtg ctgattctct ctgggtcgat tgaatttatc
     4261 ccgatgcagg tgatccctat cgccgggatg attgccggta acgccatggt agcggtgggg
     4321 ttgtgttaca acaatttagg gcaacgggtc attagcgaac agcaacagat tcaggagaaa
     4381 ctgagtcttg gtgcgacgcc gaagcaggct tcagcgatat tgattcgcga cagtattcgc
     4441 gcggctttaa ttccgacggt cgattcagca aaaacggttg gcttagtgag tttacctgga
     4501 atgatgtccg ggctgatatt tgccgggatt gatccggtga aggcgattaa atatcagatt
     4561 atggtgacct ttatgctgct ctcaaccgcc agcttgtcga ccattattgc ctgctattta
     4621 acctatcgta agttttataa ttcgcgccac cagttggtgg tgacgcaatt gaagaagaaa
     4681 tgatgcgatg ccggatgcgc agcatcgcat ccggcgttgt ggttcatgtg ccgatcaacg
     4741 gaatatcaat acaacaatgc atacagctgg cggcgatact tcgacgccag tgcatcaccc
     4801 gtacccagcg cagcgaggat ctcctgaaac gttttacgcg tctgaccgtc tgcggcggtg
     4861 agatctttac gcagatgccc gaacagcaac tccagcgcct cttcattgcg cccaacctgg
     4921 tgcagttgca gagccagttg cgtcgccagt gcggcatctt ctggattctc cgccacctgc
     4981 tgttgcaact gttgaatttc cggcgtatca gccgcctgct tcagcagttc gatttgcgcc
     5041 accagcccct ggtagcgggt gtcctgatcc tgcaacggaa tggttttcag caccgtttcc
     5101 gcatcttcag aacggttcag cgcaatcagc gtttctgcca gcagcaggcc gatctccccg
     5161 ttctgattcg acaactgcca ggcgtctttc agcaatggca gggcatcggt gtaattgcct
     5221 tcctgcatca gttgcatcgc ctgctgcgct ttcagctctt cttcgcgcgg cagcacttta
     5281 tccagcaggg cgcggatcgc ctcttccggt tgcggcccct ggaagccatc taccggttgc
     5341 ccgttctgga acagatacac ggtcggaatc gcgcgcagac caaactgggc ggcaatcatc
     5401 tgctccgcgt cgcagtccag cttcgccaga ataaattgcc cgttgtactg cgccgcgagg
     5461 ctttccagga tcggggttaa ctgcaaacag tgctggctac gttcagacca aaaatagaac
     5521 agcaccggag tggtcatcga ctgttcaaga acctgttgca ggttagattc gttaatgttg
     5581 acaatatttt ctacggacat ggagtcgctc tctgttgtcg atattttctt tgacatgggg
     5641 gcttaagcgc gcgcttcaac tcacccctgc aatattttgt ccatcacgcg ccccggcagc
     5701 aggcgcttga gcaccattac tgcccaggtc accagcgtta ccggatagcg catcttcggc
     5761 ttctcgctaa taaaagcatg gcgtactttg tccaccaccg cttccggtcc caacgtaaag
     5821 cgggcggcga tgccgggatt ttcgactggt ttatcacttt gcgtctggtt gacgttgtcg
     5881 gtgaagcgag tacgaatggg accgggttcg atcaggctga ctttaattcc gctgtggcgc
     5941 agctccatgc gcagtgcgtc tgaccacgcc tccagcgcat atttactggc cgcgtaagcg
     6001 ccacgacccg gcgtggagat taatcccatc accgatgatg tcatcacaat acgcccttca
     6061 ccgtgcggta acatcgcggg taacaggcgc atggtgagct ggtgtgcgcc gaaaaagttg
     6121 gcggaaaact gctgttccat ctgcgcacgg ctgatggtgg aaagggggcc atacatgccg
     6181 aatccggcat tgttaaagat cccatacaga caattatcgg tcagggcgat cacctcgtct
     6241 gctgcgcgat caacactttc tggtgaatcc agatcgatca acacgccggt aaatcccatg
     6301 ttgttcatac gctcaacatc atccggtttt cggcaacctg ccagcacatg aaaaccctgg
     6361 cgttttaatt cgagcgcgct ttccaggcca attccactgg aacatccggt aattaagacc
     6421 gatttttgca taactttacc tgtcaggatc tccgttgctt tatgagtcat gatttactaa
     6481 aggctgcaac tgcttcgcca tccagtcggc aataaacggc tgggcgtcgc ggttgggatg
     6541 aataccgtca tcctgcatcc attgtggctt gaggtagacc tcttccataa aaaagggcag
     6601 cagcggaaca tcaaactctt tggcgagttt ggggtaaatg gcgctaaagg cttcattata
     6661 acggcgacca tagtttgcag gcagacgtat ttgcattaac aatggttcag cgttggcggc
     6721 tttgacatcc tgcaaaatct ggcgcagcgt ttgctcggtt tgctgtggct gaaaaccacg
     6781 caaaccgtca ttgccgccca gttcaaccag cacccagcgc ggctgatgct gtttcagcag
     6841 agccggaagg cgcgccagtc cttgttgcga ggtgtcgccg ctgatgctgg cattaactac
     6901 cgacgtttta ctctgccact tatcattcaa caaggcaggc caggccgcgc tggcagacat
     6961 tcgatacccg gcgctcaggc tatcacccag aatcaataac gtgtccgctg cggcggcacg
     7021 gaaggttaac aggaccagga acaggaaggg caaatgccag cggaaaacat tgttgaagtt
     7081 catcatctta agaagtccgt cggtcagggg gagcatgaac tctccatcct caccggagtt
     7141 gagctggttg tcaaacgtgg cgagaccatc gcactggtgg gcgaatcggg atcgggtaag
     7201 tcaaccttgc tggcgatcct cgccgggctt gatgacggca gcagtggcga agtgagtctg
     7261 gtgggacaac cgctacataa tatggacgaa gaagcgcggg caaagttgcg cgcgaagcac
     7321 gtcggctttg tttttcagtc atttatgtta attcctaccc ttaacgcgct ggaaaatgtc
     7381 gagcttccgg ctctgctgcg cggtgagagt agcgcggaaa gtcgtaacgg ggcgaaagcg
     7441 ttgctcgaac agttagggct gggtaaacgt ctggatcatc ttccggcaca gctttccggc
     7501 ggtgaacagc aacgagtggc gctggcacga gcctttaatg gtcgacctga tgtgctgttt
     7561 gccgacgaac ccaccggcaa ccttgaccgc cagacgggcg ataaaattgc cgacctgctg
     7621 ttttccctca accgtgaaca tggcaccacg ttgattatgg tgactcacga cctgcaactg
     7681 gcggcacgct gcgaccgctg cttacggctg gtgaacgggc agttgcagga ggaagcatga
     7741 ttgcacgttg gttctggcgc gaatggcgct cgccgtcgct attaattgtc tggctggcgc
     7801 taagcctggc ggtggcctgc gtgctggcgc tgggcaatat cagcgatcgc atggagaagg
     7861 gcttaagcca gcaaagccgt gagtttatgg cgggcgatcg ggcgttgcgc agttcgcgcg
     7921 aagtgccgca agcatggctg gaggaagcgc aaaagcgcgg cctgaaagtc ggcaagcagc
     7981 tgactttcgc caccatgaca tttgcaggcg acacaccgca gctggcgaac gtcaaagcgg
     8041 tggatgatat ctacccgatg tatggcgatc tgcaaactaa tccccctggc ctgaaaccgc
     8101 aggcgggcag cgtattgctg gccccacgcc tgatggcact gcttaacctg aaaacgggcg
     8161 acaccattga cgtgggcgat gccaccttgc ggattgccgg agaagtgatt caggaaccgg
     8221 attccggttt taaccccttc cagatggctc cgcgtctgat gatgaatctg gcggatgtcg
     8281 ataaaaccgg agccgtgcag ccggggagtc gggtcacctg gcgttataaa ttcggcggca
     8341 acgagaacca gctcgacggc tatgagaaat ggttgttacc tcagcttaaa cccgaacaac
     8401 gctggtacgg tctggaacag gacgaaggcg cgctggggcg atcgatggaa cgctcgcaac
     8461 agttcctgct gctttcggcg cttctgacct tgctgctggc agtggcagcg gtggcggtag
     8521 cgatgaatca ttactgtcgc agtcgctacg atctggtggc gatcctcaaa acgctggggg
     8581 cagggcgagc gcaactgcgt aagctaatcg tcggtcagtg gttgatggtg ctggcgcttt
     8641 cagccgttac cggtggggcg ataggcctgt tgttcgaaaa cgtgttgatg gtgctgctca
     8701 agcccgttct gcctgctgca ctaccgccag ccagcctctg gccgtggctg tgggcgctgg
     8761 gtaccatgac ggtcatctcg ctgctggtgg ggctacgacc gtaccgcctg ttgctggcaa
     8821 cgcagccttt acgcgtatta cgtaatgatg tggtagcgaa cgtctggccg ctgaagtttt
     8881 atctgccgat tgtcagtgtg gtggttgtgc tgctgctcgc cggattaatg ggtggcagca
     8941 tgctgctttg ggcggtgctg gcgggcgcgg tagtactggc tttgctgtgc ggtgtgctgg
     9001 gctggatgct gctgaatgta cttcgccgca tgacgctgaa atcgctacct ctgcgcctgg
     9061 cggttagccg cctgttacgt cagccgtggt caacattaag ccagctttcg gcattttcgc
     9121 tctcctttat gctgctggcg ctgttgctgg tattgcgtgg cgatctgctt gaccgctggc
     9181 agcagcagct acctccagaa agcccgaact actttttaat taacattgcc acagaacagg
     9241 tagaaccgct aaaagcgttc ctcgcggaac atcagatagt cccggaatca ttttatccgg
     9301 tagtgcgggc gcggctgacg gcgattaacg ataagccgac agaaggtaat gaagatgagg
     9361 cgcttaaccg cgaactcaat cttacctggc aaaatacgcg acccgatcat aatccgattg
     9421 tcgccggtaa ctggccgcca aaagccgatg aagtgtcgat ggaagagggg ctggcgaaac
     9481 gcttaaacgt tgccctcggt gataccgtga cttttatggg cgatacccag gagttccgcg
     9541 ctaaagtgac cagcctgcgc aaagtggact gggaaagtct gcggcctaat ttctatttta
     9601 ttttccctga aggggcatta gacgggcaac cgcagagctg gcttaccagt ttccgctggg
     9661 agaatggcaa cggcatgttg acacaactca accgccagtt cccgaccatt agcctgttag
     9721 atattggcgc gattttaaaa caggtcggtc aggtgctgga gcaggtaagt cgggcgctgg
     9781 aggtgatggt ggtgctggtc accgcctgcg gtatgttgct gttgctggca caggtgcagg
     9841 tgggaatgcg ccagcgtcat caggagctgg tggtatggcg cacactcggt gcggggaaaa
     9901 aactgctgcg cacgacattg tggtgtgagt tcgccatgct tgggtttgtt tccggcctgg
     9961 tggccgcaat tggtgcggaa acggcactgg cagtgttgca ggcgaaagtg tttgatttcc
    10021 cgtgggagcc agactggcga ttgtggattg ttctgccgtg cagcggtgcg ctgctgctgt
    10081 cgcttttcgg cggctggctg ggtgcgcgac tggttaaggg taaggcgctg ttcaggcagt
    10141 ttgcggggtg atgagagtaa ataaggggcg ttctggttga atggaacgcc ttgtttaaat
    10201 ta
//
LOCUS       DAZKHF020000165        10191 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2201S31241
            SAMN43267800-rid25106413.denovo.166, whole genome shotgun sequence.
ACCESSION   DAZKHF020000165 DAZKHF020000000
VERSION     DAZKHF020000165.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267800
            Sequence Read Archive: SRR30307263
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 10191)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10191)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 10191)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350581.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350581.2
            Genome Coverage       :: 151x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:21:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,652
            CDSs (total)                      :: 5,557
            Genes (coding)                    :: 5,320
            CDSs (with protein)               :: 5,320
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 237
            CDSs (without protein)            :: 237
            Pseudo Genes (ambiguous residues) :: 0 of 237
            Pseudo Genes (frameshifted)       :: 102 of 237
            Pseudo Genes (incomplete)         :: 130 of 237
            Pseudo Genes (internal stop)      :: 52 of 237
            Pseudo Genes (multiple problems)  :: 41 of 237
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10191
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267800-rid25106413.denovo.166"
                     /serotype="O 26:H11"
                     /isolate="2201S31241"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-01"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            59..1741
                     /gene="ydeN"
                     /locus_tag="ACHOSC_RS16540"
                     /old_locus_tag="pgaptmp_003308"
     CDS             59..1741
                     /gene="ydeN"
                     /locus_tag="ACHOSC_RS16540"
                     /old_locus_tag="pgaptmp_003308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416015.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase-like hydrolase/transferase"
                     /protein_id="HGU4549199.1"
                     /translation="MKSALKKSVVSTSISLILASGMAAFAAHAADDVKQKATKTNVAF
                     SDFTPTEYSTKGKPNIIVLTMDDLGYGQLPFDKGSFDPKTMENREVVDTYKIGIDKAI
                     EAAQKSTPTLLSLMDEGVRFTNGYVAHGVSGPSRAAIMTGRAPARFGVYSNTDAQDGI
                     PLTETFLPELFQNHGYYTAAVGKWHLSKISNVPVPEDKQTRDYHDNFTTFSAEEWQPQ
                     NRGFDYFMGFHAAGTAYYNSPSLFKNRERVPAKGYISDQLTDEAIGVVDRAKTLDQPF
                     MLYLAYNAPHLPNDNPAPDQYQKQFNTGSQTADNYYASVYSVDQGVKRILEQLKKNGQ
                     YDNTIILFTSDNGAVIDGPLPLNGAQKGYKSQTYPGGTHTPMFMWWKGKLQPGNYDKL
                     ISAMDFYPTALDAADISIPKDLKLDGVSLLPWLQDKKQGEPHKNLTWITSYSHWFDEE
                     NIPFWDNYHKFVRHQSDDYPHNPNTEDLSQFSYTVRNNDYSLVYTVENNQLGLYKLTD
                     LQQKDNLAAANPQVVKEMQGVVREFIDSSQPPLSEVNQEKFNNIKKALSEAK"
     gene            1793..2950
                     /gene="ydeM"
                     /locus_tag="ACHOSC_RS16545"
                     /old_locus_tag="pgaptmp_003309"
     CDS             1793..2950
                     /gene="ydeM"
                     /locus_tag="ACHOSC_RS16545"
                     /old_locus_tag="pgaptmp_003309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416014.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0018209 - peptidyl-serine modification
                     [Evidence IEA];
                     GO_process: GO:0046500 - S-adenosylmethionine metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic sulfatase maturase"
                     /protein_id="HGU4549200.1"
                     /translation="MHVTAKPSSFQCNLKCDYCFYLEKESQFTHEKWMDDSTLKEFIK
                     QYIAASGNQVYFTWQGGEPTLAGLDFFRKVIHYQQRYAGQKRIFNALQTNGILLNNEW
                     CAFLKEHEFLVGISIDGPQELHDRYRRSNSGNGTFAKVIAAIERLKSYQVEFNTLTVI
                     NNVNVHYPLEVYHFLKSIGSKHMQFIELLETGTPNIDFSGHSENTFRIIDFSVPPTAY
                     GKFMSTIFMQWVKNDVGEIFIRQFESFVSRFLGNGHTSCIFQESCKDNLVVESNGDIY
                     ECDHFVYPQYKIGNINKSELKTMNSVQLTAQKKRISAKCQQCAYKPICNGGCPKHRIT
                     KVNNETVSYFCEGYKNLFSTMVPYMNAMVELAKNRVPLYHIMDVAKQMENN"
     gene            complement(2916..3038)
                     /locus_tag="ACHOSC_RS16550"
                     /old_locus_tag="pgaptmp_003310"
     CDS             complement(2916..3038)
                     /locus_tag="ACHOSC_RS16550"
                     /old_locus_tag="pgaptmp_003310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4549201.1"
                     /translation="MTKHPTGIYVGCLVKVIRRRLRMELKGRVINYSPFVLQHP"
     gene            3241..4926
                     /gene="yddA"
                     /locus_tag="ACHOSC_RS16555"
                     /old_locus_tag="pgaptmp_003311"
     CDS             3241..4926
                     /gene="yddA"
                     /locus_tag="ACHOSC_RS16555"
                     /old_locus_tag="pgaptmp_003311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein/permease"
                     /protein_id="HGU4549202.1"
                     /translation="MITIPITFCMLIAKYLCLLKPFWLRKNNKTSVLLIIIILAMILG
                     VVKIQVWLNDWNNDFFNALSQKETDKLWQLVLWFPALLGIFVLISVNKTWLIKLLTIR
                     WREWLTDYYLNRWFADKNYYFTQIYGEHKNTDNPDQRIAEDILLLISKTLSLSFGFIQ
                     SLSMLITFTVILWQSAGTLSFTVGGTEWNIQGYMVYTVVLIVIGGTLFTHKVGKRIRP
                     LNVEKQRSEATFRTNLVQHNKQAELIALSNAESLQRQELSDNFHTIKENWHRLMNRQR
                     WLDYWQNIYSRSLSVLPYFLLLPQFISGQINLGGLMKSRQAFMLVSNNLSWFIYKYDE
                     LAELAAVIDRLYEFHQLTEQRPTNKPKNCQHAVQVADASIRTPDNKIILENLNFHVSP
                     GKWLLLKGYSGAGKTTLLKTLSHCWPWFKGDISSPADSWYVSQTPLIKTGLLKEIICK
                     ALPLPVDDKSLSEVLHQVGLGKLAARIHDHDRWGDILSSGEKQRIALARLILRRPKWI
                     FLDETTSHLEEQEAIRLLRLVREKLPTSGVIMVTHQSGVWNLADDICDISAVL"
     gene            4964..7336
                     /gene="yddB"
                     /locus_tag="ACHOSC_RS16560"
                     /old_locus_tag="pgaptmp_003312"
     CDS             4964..7336
                     /gene="yddB"
                     /locus_tag="ACHOSC_RS16560"
                     /old_locus_tag="pgaptmp_003312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor plug domain-containing
                     protein"
                     /protein_id="HGU4549203.1"
                     /translation="MKRVLIPGVILCGADVAQAVDDKNMYMHVFEEMTVYAPVPVPVN
                     GNTHYTSESIERLPTGNGNISDLLRTNPAVRMDSTQSTSLNQGDIRPEKISIHGASPY
                     QNAYLIDGISATNNLNPANESDASSATNISGMSQGYYLDVSLLDNVTLYDSVVPVEFG
                     RFNGGVIDAKIKRFNADDSKVKLGYRTTRSDWLTSHIDENNKSAFNQGSSGSTYYSPD
                     FKKNFYTLSFNQELADNFGVTAGLSRRQSDITRADYVSNDGIVAGRAQYKNVIDTALS
                     KFTWFASDRFTHDLTLKYTGSSRDYNTSTFPQSDREMGNKSYGLAWDMDTQLAWAKLR
                     TTVGWDHISDYTRHDHDIWYTELSCTYGDITGRCTRGGLGHISQAVDNYTFKTRLDWQ
                     KFAVGDVSHQPYFGAEYIYSDAWTERHNQSESYVINAAGKKTNHTIYHKGKGSLGIDN
                     YTLYMADHISWRNVSLMPGVRYDYDNYLSNHNISPRFMTEWDIFADQTSMITAGYNRY
                     YGGNILDMGLRDIRNSWTESVSGNKTLTRYQDLKTPYNDELAMGLQQKIGKNVIARAN
                     YVYREAHDQISKSSRTDSATKTTITEYNNDGKTKTHSFNLSFELAEPLHISQVDINPQ
                     IVFSYIKSKGNLSLNNGYEESNTGDNQVVYNGNLVSYDSVPVADFNNPLKISLNMDFT
                     HQPSGLVWANTLAWQEARKARIILGKTNAQYISEYSDYKQYVDEKLDSSLTWDTRLSW
                     TPQFLKQQNLTISADILNVLDSKTAVDTTNTGVATYASGRTFWLDVSMKF"
     gene            7393..10176
                     /gene="pqqL"
                     /locus_tag="ACHOSC_RS16565"
                     /old_locus_tag="pgaptmp_003313"
     CDS             7393..10176
                     /gene="pqqL"
                     /locus_tag="ACHOSC_RS16565"
                     /old_locus_tag="pgaptmp_003313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310126.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M16 family metallopeptidase"
                     /protein_id="HGU4549204.1"
                     /translation="MRNLCFLLTLVATLLLPGRLIAAALPQDEKLITGQLGNGLRYMI
                     YPHAHPKDQVNLWLQIHTGSLQEEDNERGVAHFVEHMMFNGTKTWPGNKVIETFESMG
                     LRFGRDVNAYTSYDETVYQVSLPTTQKQNLQQVMAIFSEWSNAATFEKLEVDAERGVI
                     TEEWRAHQDAKWRTSQARRPFLLANTRNLDREPIGLMDTVATVTPAQLRQFYQRWYQP
                     NNMTFIVVGDIDSKEALALIKDNLSKLPANKAAENRVWPTKAENHLRFNIINDKENRV
                     NGIALYYRLPMVQVHDEQSFIEQAEWSMLVQLFNQRLQERIQSGELKTISGGTARSVK
                     IAPDYQSLFFRVNARDDNMQDAANALMAELATIDQHGFSAEELDDVKSTRLTWLKNAV
                     DQQAERDLRMLTSRLASSSLNNTPFLSPEETYQLSKRLWQQITVQSLAEKWQQLRKNQ
                     DAFWEQMVNNELAAKKALSPAAILALEKEYANKKLAAYIFPGRNLSLTVDADPQAEIS
                     SKETLAENLTSLTLSNGARVILAKSAGEEQKLQITAVSNKGDLSFPAQQKSLIALANK
                     AVSGSGVGELSSSSLKRWSAENSVTMSSKVSGMNTLLSVSARTNNPEPGFQLINQRIT
                     HSTINDNIWASLQNAQIQALKTLDQRPAEKFAQQMYETRYADDRTKLLQENQIVQFTA
                     ADALAADRQLFSSPADITFVIVGNVSEDKLVALITRYLGSIKHSDSPLAAGKPLTRAT
                     DNASVTVKEQNEPVAQVSQWKRYDSRTPVNLATRMALDAFNVALAKDLRVNIREQASG
                     AYSVSSRLSVDPQAKDISHLLAFTCQPERHDELLTLANEVMVKRLAKGISEQELNEYQ
                     QNVQRSLDIQQRSVQQLANTIVNSLIQYDDPAAWTEQEQLLKQMTVENVNTAVKQYLS
                     HPVNTYTGVLLPK"
BASE COUNT         2993 a         2251 c         2246 g         2701 t
ORIGIN      
        1 acttatttct cttcgtaaaa ttacttcgga attaagtaca ataagaagag gaacatttat
       61 gaagtctgca ttaaagaaaa gtgtcgtaag tacctcgata tctttgatac tggcatctgg
      121 tatggctgca tttgctgctc atgcggcaga tgatgtaaag cagaaagcaa ccaaaacaaa
      181 cgttgctttc tcagacttta cgccgactga atacagtacc aaaggaaagc caaatattat
      241 cgtactaacc atggatgatc ttggttatgg acaacttcct tttgataagg gatcttttga
      301 cccaaaaaca atggaaaatc gtgaagttgt cgatacctac aaaataggga tagataaagc
      361 cattgaagct gcacaaaaat caacgccgac gctcctttca ttaatggatg aaggcgtacg
      421 gtttactaac ggctatgtgg cacacggtgt ttccggcccc tcccgtgccg caataatgac
      481 cggtcgagct cccgcccgct ttggtgtcta ttccaatacc gatgctcagg atggtattcc
      541 gctaacagaa actttcttgc ctgaattatt ccagaatcat ggttattaca ctgcagcagt
      601 aggtaaatgg cacttgtcaa aaatcagtaa tgtgccggta ccggaagata aacaaacgcg
      661 tgactatcat gacaacttca ccacattttc tgcggaagaa tggcaacctc aaaaccgtgg
      721 ctttgattac tttatgggat tccacgctgc aggaacggca tattacaact ccccttcact
      781 gttcaaaaat cgtgaacgtg tccccgcaaa aggttatatc agcgatcagt taaccgatga
      841 ggcaattggc gttgttgatc gtgccaaaac acttgaccag ccttttatgc tttacctggc
      901 ttataatgct ccgcacctgc caaatgataa tcctgcaccg gatcaatatc agaagcaatt
      961 taataccggt agtcaaacag cagataacta ctacgcttcc gtttattctg ttgatcaggg
     1021 tgtaaaacgc attctcgaac aactgaagaa aaacggacag tatgacaata caattattct
     1081 ctttacctcc gataatggtg cggttatcga tggtcctctg ccgctgaacg gggcgcaaaa
     1141 aggctataag agtcagacct atcctggcgg tactcacacc ccaatgttta tgtggtggaa
     1201 aggaaaactt caacccggta attatgacaa gctgatttcc gcaatggatt tctacccgac
     1261 agctcttgat gcagccgata tcagcattcc aaaagacctt aagctggatg gcgtttcctt
     1321 gctgccctgg ttgcaagata agaaacaagg cgagccacat aaaaatctga cctggataac
     1381 ctcttattct cactggtttg acgaggaaaa tattccattc tgggataatt accacaaatt
     1441 tgttcgccat cagtcagacg attacccgca taaccccaac actgaggact taagccaatt
     1501 ctcttatacg gtgagaaata acgattattc gcttgtctat acagtagaaa acaatcagtt
     1561 aggtctctac aaactgacgg atctacagca aaaagataac cttgccgccg ccaatccgca
     1621 ggtcgttaaa gagatgcaag gcgtggtaag agagtttatc gacagcagcc agccaccgct
     1681 tagcgaagta aatcaggaga agtttaacaa tatcaagaaa gcactaagcg aagcgaaata
     1741 actaaacctt catgcggcgg aattttccgc cgccttattg agcgagatag cgatgcacgt
     1801 tacagccaag ccctccagtt ttcaatgtaa tctcaaatgt gattactgtt tttaccttga
     1861 aaaagagtcg cagtttactc atgaaaaatg gatggatgac agcactctga aagagttcat
     1921 caaacaatat atcgcagcgt ctggcaatca ggtctatttt acctggcaag gcggtgaacc
     1981 cactctggct ggcctggatt ttttccgtaa agttattcac tatcaacaac gctatgcagg
     2041 ccaaaaacgt atttttaatg cattacaaac gaatggcatt ttattgaata atgaatggtg
     2101 tgcttttctc aaagaacatg agtttctggt tggtatctcg atcgatggcc cccaggagct
     2161 acatgaccgt tacagacgca gtaattcagg taacggtact tttgcaaaag tgatagcagc
     2221 catcgagcgt ctgaaatcat atcaagttga gtttaatacg ttaaccgtca ttaataacgt
     2281 taatgtccat taccctcttg aggtttatca ttttttaaaa tctatcggca gtaaacatat
     2341 gcaatttatc gaattgctcg aaaccgggac gccgaatatt gatttcagtg gtcatagtga
     2401 gaacacattc cgtatcattg atttttctgt gcctcccacg gcttatggca agtttatgtc
     2461 aaccattttt atgcaatggg ttaaaaacga tgtgggtgaa attttcattc gtcagtttga
     2521 aagctttgtc agccgttttt tggggaatgg gcataccagt tgtattttcc aggagtcctg
     2581 caaggataat ctggttgttg aaagtaatgg agacatttac gaatgcgacc attttgtcta
     2641 tccacagtac aaaattggaa acattaataa atctgaactc aaaacgatga acagtgtaca
     2701 actgacagcg caaaaaaaac ggatttcagc gaaatgtcag caatgtgcat ataaacctat
     2761 ctgcaatggc ggttgtccta agcatcgtat tactaaagta aacaatgaga ctgtttctta
     2821 tttttgcgaa ggttataaaa accttttttc aaccatggta ccttatatga acgccatggt
     2881 ggagttagct aagaacagag taccgcttta tcacattatg gatgttgcaa aacaaatgga
     2941 gaataattaa taacacgccc ttttagctcc attcttagcc tccttcttat gaccttaaca
     3001 aggcacccga cataaattcc tgtcgggtgt ttcgtcatca tcatcttccc ttgcgaaaat
     3061 cctggcttac gtggtatgcg tcagggcatt agcaataccc ttgattatca atagcataac
     3121 cataactaat gaaaatgaaa tatttcttta tatttcatat tgttaagtga tacttaaata
     3181 aatttcatca gtaaatcatt gccatagata atcattggat ggcacaatca attttcgcta
     3241 atgataacca ttcccattac gttttgtatg ttgatagcaa agtatctctg tctgctgaag
     3301 cccttttggc tgcgtaagaa taataaaact tcagtgttgt taatcattat tatcctcgcc
     3361 atgatccttg gcgtggttaa aattcaggtc tggttaaacg actggaataa tgattttttc
     3421 aatgcgctaa gccagaaaga aaccgataag ctttggcagc ttgtcctttg gttccctgcc
     3481 ctgctgggga tctttgtatt aatatccgta aacaaaacat ggttaattaa actactgact
     3541 attcgctggc gcgaatggtt aacagattac taccttaatc ggtggtttgc agataaaaac
     3601 tactacttca cgcagatcta cggcgagcat aaaaatacag ataaccctga ccaacgtatc
     3661 gctgaagaca ttctcttact gatcagcaaa acgctcagcc tttcatttgg cttcatccag
     3721 tcacttagca tgctgatcac ctttaccgtt attctttggc aaagcgcggg tacgctctca
     3781 ttcactgttg gtggaacaga atggaatatc caggggtata tggtctatac cgttgtgctc
     3841 attgtgatcg gtggaacttt atttacccat aaggttggta aacgtattcg tccgcttaat
     3901 gtggaaaaac aacgtagcga agcgacattt cgaacaaatc ttgtgcagca taataagcag
     3961 gcagaactga ttgcgctaag caatgctgag tctctacaac gccaggagtt gagcgacaat
     4021 tttcatacca ttaaagagaa ctggcatcgt ttaatgaatc gtcagcggtg gcttgattac
     4081 tggcagaata tttattcgcg ctcgcttagc gttcttccct actttctgtt attgccgcag
     4141 tttattagcg ggcagatcaa tctgggcgga ctgatgaaat cgcgccaggc atttatgctg
     4201 gtatcgaaca atttaagctg gtttatttat aaatatgacg aacttgctga actggctgcg
     4261 gttatcgatc gtttgtatga gttccatcaa ctcaccgaac agcgccctac gaataaacct
     4321 aaaaattgcc aacatgcggt acaagtggct gacgcgagta ttcgtactcc tgataataag
     4381 atcatattag agaacctgaa ctttcatgtt tcgccaggca aatggctatt actgaaaggt
     4441 tactctggtg cgggaaaaac cacactgctt aaaacattat ctcactgctg gccgtggttt
     4501 aaaggtgata tttcttctcc tgctgacagt tggtatgtgt cacaaacacc gttaatcaaa
     4561 accggcttac tgaaagagat tatttgtaaa gcacttcccc tgcccgtaga cgataaatcg
     4621 ttgagcgaag tactgcatca ggttggtctg gggaaattgg ctgcgcgtat tcatgaccac
     4681 gatcgctggg gagatattct ttccagcggc gaaaaacaac gtatcgccct ggcacgatta
     4741 attttacgac gtccgaaatg gatatttctt gacgaaacta cctctcatct tgaggaacaa
     4801 gaggctatcc gcttactgcg tttagtgcgt gaaaaactac ccacaagcgg cgtcattatg
     4861 gttacacatc aatccggtgt ctggaacctg gcagatgata tttgtgacat tagcgcggtt
     4921 ttataaaaac aataaaagcc ctgctgtacg gagatataaa taaatgaagc gagttcttat
     4981 tcctggcgtc attttatgtg gcgctgatgt ggcgcaggcc gtcgatgaca aaaacatgta
     5041 catgcatgtt tttgaagaga tgacggtcta tgctcctgtc cctgtacccg taaacggcaa
     5101 cacgcattac accagtgaaa gcatcgagcg tttaccgacc gggaatggca atatcagcga
     5161 tctgctgaga accaaccctg cggtacgcat ggattcaacg caaagtacct cgttgaacca
     5221 gggagatatt cgcccggaga aaatctctat tcacggtgcg tcgccctatc agaatgccta
     5281 tttgattgac ggtattagtg ccactaataa cttgaaccca gcgaatgagt ccgatgccag
     5341 tagtgcaacc aatattagcg ggatgtcaca ggggtattat cttgatgtca gcttactgga
     5401 caatgtgacg ctttatgaca gtgttgtgcc ggttgaattt ggtcgcttca atggcggggt
     5461 aattgatgca aagatcaaac gcttcaacgc tgatgatagc aaggtgaaat tgggttatcg
     5521 cactacgcgt tcggactggt taacatcgca tatcgatgag aataacaaga gcgcatttaa
     5581 tcaaggctct tcaggaagta cttattactc cccagatttt aaaaagaact tttatacctt
     5641 gtcgtttaat caggaactcg ctgataactt cggcgttacc gccggtttat cgcgccgcca
     5701 gtctgatatc acccgcgcgg attatgtttc gaatgacggc attgtcgccg gtcgggcaca
     5761 gtataaaaac gttatcgata ctgcattgag caaatttacc tggtttgcca gcgaccgctt
     5821 tacccacgat ttaaccttaa aatataccgg ctccagccgt gattataata ccagcacctt
     5881 cccgcagtct gatcgcgaaa tgggtaataa atcctacggt ctggcatggg atatggatac
     5941 gcagctcgca tgggccaaac tgcgtaccac cgttggttgg gatcatatta gtgattatac
     6001 ccgtcacgat catgacatct ggtacaccga actttcatgt acatatggtg atattacagg
     6061 gcgttgtacc cgtggcggat taggacacat ttcccaggct gtagataatt acaccttcaa
     6121 aacacgcctg gactggcaaa aattcgccgt gggtgatgtt tcgcatcaac cctacttcgg
     6181 cgcggaatac atctattccg atgcatggac tgaacgccat aaccagtctg aatcctatgt
     6241 gattaatgct gcaggaaaga aaactaacca taccatttac cataaaggta aaggcagcct
     6301 gggaattgac aactacacgc tgtatatggc ggatcacatt agctggcgga atgtgtcgtt
     6361 aatgcccggt gtgcgttatg actatgacaa ctatctgtca aaccacaata tctccccgcg
     6421 ctttatgacg gaatgggata tttttgctga tcaaacctca atgattacag caggttataa
     6481 ccgttactat ggcgggaata ttcttgatat gggattacgt gatatccgca atagctggac
     6541 ggaatcggta tcaggcaata aaaccctgac gcgttatcag gatttgaaaa cgccttataa
     6601 cgatgaactg gcaatgggat tgcagcagaa aatcggtaag aacgttattg cgcgcgcaaa
     6661 ctatgtttac cgtgaagcgc atgatcaaat cagcaaaagc agtcgtaccg acagcgcgac
     6721 taaaaccacc attactgaat ataacaacga cggcaaaaca aaaacgcatt cgttcaacct
     6781 cagttttgaa ctggccgaac ccctgcatat cagccaggta gatattaacc cgcaaattgt
     6841 ctttagctat atcaagagca agggcaactt gtcgttaaac aatggttatg aggagagcaa
     6901 taccggtgat aaccaggtgg tttataacgg taatctggtt tcttacgata gcgttccagt
     6961 ggcagatttt aataacccat taaagatctc cttaaacatg gatttcacgc atcaaccgag
     7021 cgggttagtg tgggcgaata cgctggcctg gcaagaagcg cgtaaagctc gcattatcct
     7081 gggtaagaca aatgcgcaat acatcagcga atattcagat tacaagcagt atgttgacga
     7141 aaaactggat agcagcctga cctgggacac ccgcttgtcc tggacgccac aatttctgaa
     7201 acaacaaaac ctgacgatca gtgccgatat tctcaatgta ttggatagca aaaccgctgt
     7261 tgatacaacg aataccggtg tggcgaccta cgccagtggc cgtactttct ggcttgatgt
     7321 cagcatgaaa ttttaatagt tcggatttta cttaaccggg taaattcgcc cggttttcgc
     7381 atggagataa ttatgagaaa cctctgtttc ttactgacgt tagtggcaac tctgttgctc
     7441 cccgggcggc tgattgccgc cgccttaccg caggatgaaa agttaattac cgggcaactg
     7501 ggcaatggct tgcgatatat gatttatccg catgctcatc caaaagatca ggtaaattta
     7561 tggctgcaaa ttcataccgg ttcattgcag gaagaagaca atgagcgcgg cgtggctcat
     7621 tttgtagaac atatgatgtt taacggcaca aaaacatggc cgggtaataa agtcatcgaa
     7681 acatttgagt caatgggcct gcgttttggt cgcgatgtta atgcctatac cagctatgac
     7741 gaaacggtgt atcaggtgag tttgccgact acgcagaaac aaaatctgca acaagtgatg
     7801 gcaatcttca gtgaatggag taatgccgca acctttgaaa aactcgaagt agacgctgaa
     7861 cgtggcgtaa ttactgagga atggcgtgcc catcaggatg cgaaatggcg cacctctcag
     7921 gcgcgccgcc ctttcctgct ggctaatacc cgtaatttag accgtgaacc tatcggcctg
     7981 atggatactg tcgccacggt cacaccggca caattgcgcc aattttatca acgctggtat
     8041 caaccaaata atatgacctt tatcgtggtt ggcgatatcg acagtaaaga agcgctggcg
     8101 ctgataaagg ataatttaag taagcttccg gctaacaaag cagctgaaaa tcgcgtctgg
     8161 ccgacaaaag ccgaaaacca cctgcgcttt aatatcatta atgataaaga aaaccgggtg
     8221 aacggcatcg cactctatta tcgcctgcca atggtacaag tgcacgatga gcaaagcttt
     8281 atcgaacaag ctgaatggag catgttagtt cagctgttca atcaacgtct gcaggaacgc
     8341 atacagtcgg gcgagttgaa gactatttct ggcggcactg cgcgcagcgt taaaattgca
     8401 cccgattatc agtcgctgtt tttccgtgta aatgcacgag acgataatat gcaggatgct
     8461 gcgaatgcat taatggcaga gttggcaact attgatcagc atggtttttc tgctgaagaa
     8521 ctcgatgatg ttaaatctac ccgcctcacc tggctgaaaa atgcggttga tcagcaagct
     8581 gaacgtgatt tacgtatgct gaccagtcgc ctggcatcca gctcattaaa taatacgccg
     8641 ttcttgtcgc cggaagagac atatcaactt tcgaaacgtc tgtggcagca aattaccgtg
     8701 caaagtctgg cggaaaaatg gcagcagtta agaaagaacc aggacgcatt ttgggagcaa
     8761 atggtaaaca atgagcttgc cgccaaaaaa gcattgtctc ctgcggctat cctggcgctg
     8821 gaaaaagagt acgccaacaa aaagctggcg gcttacatct tcccaggcag aaatttatcg
     8881 ttaacagtag acgctgaccc acaggcggaa attagcagca aagaaacgct ggctgagaat
     8941 ctgacatcat taacactttc caatggtgcc agggttattc tggcaaaatc cgcgggtgaa
     9001 gagcaaaagc tacaaattac tgccgtatcg aataaaggcg atttaagttt ccctgcgcag
     9061 caaaaatcac ttatcgcgct ggcaaataaa gcagtaagcg gaagcggcgt tggtgagctc
     9121 tcctcttcca gcctgaaacg ctggagtgcg gaaaattcgg taaccatgag cagtaaagtc
     9181 agtggcatga atacgttgct ctccgttagc gcgcggacta ataaccctga acctggtttt
     9241 cagttgatta accagcgaat cacccacagc acgattaacg acaatatttg ggcatcgcta
     9301 caaaatgctc aaattcaggc gttgaaaacg ctcgaccagc gtccagcgga gaaattcgcc
     9361 cagcagatgt atgagacgcg ctatgctgat gaccgcacga aattactgca agaaaatcag
     9421 attgtacagt ttactgccgc agatgcgctg gctgccgatc gccagttgtt ttcatctcca
     9481 gcggatatca cgtttgtcat tgtcggtaat gtctcagaag acaaactcgt ggcgttaatt
     9541 acgcgttact taggatcaat caaacactct gattcgccat tagccgcagg taaaccatta
     9601 actcgcgcga cggacaacgc atcggttact gtaaaagaac aaaatgaacc tgtggcacag
     9661 gtttcacagt ggaagcgtta tgattcccgg acacctgtta atctggcgac gcgtatggcg
     9721 ctcgatgctt ttaacgtcgc actggcaaaa gatctacgtg ttaatattcg tgaacaggca
     9781 tctggagcat acagcgtttc ttctcgcctc tcggttgatc ctcaggccaa agatatcagt
     9841 catttgctgg cttttacttg tcaaccagaa cgacatgatg aactgttaac gttagcgaat
     9901 gaagtgatgg ttaagcgtct ggctaaaggg atcagtgagc aagaactgaa tgaataccag
     9961 caaaacgttc agcgcagcct cgatatccaa cagcgtagcg ttcaacaatt agcgaacact
    10021 attgtaaata gtcttattca atatgacgat cctgcagcat ggactgagca ggagcaattg
    10081 ttgaaacaaa tgacggtaga gaatgttaac actgccgtta aacaatatct ttctcatccg
    10141 gtaaatactt ataccggagt attattgcca aaataataac agccccgtca a
//
LOCUS       DAZKHF020000166        10133 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2201S31241
            SAMN43267800-rid25106413.denovo.167, whole genome shotgun sequence.
ACCESSION   DAZKHF020000166 DAZKHF020000000
VERSION     DAZKHF020000166.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267800
            Sequence Read Archive: SRR30307263
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 10133)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10133)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 10133)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350581.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350581.2
            Genome Coverage       :: 151x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:21:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,652
            CDSs (total)                      :: 5,557
            Genes (coding)                    :: 5,320
            CDSs (with protein)               :: 5,320
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 237
            CDSs (without protein)            :: 237
            Pseudo Genes (ambiguous residues) :: 0 of 237
            Pseudo Genes (frameshifted)       :: 102 of 237
            Pseudo Genes (incomplete)         :: 130 of 237
            Pseudo Genes (internal stop)      :: 52 of 237
            Pseudo Genes (multiple problems)  :: 41 of 237
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10133
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267800-rid25106413.denovo.167"
                     /serotype="O 26:H11"
                     /isolate="2201S31241"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-01"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            215..1588
                     /gene="aroP"
                     /locus_tag="ACHOSC_RS16570"
                     /old_locus_tag="pgaptmp_003314"
     CDS             215..1588
                     /gene="aroP"
                     /locus_tag="ACHOSC_RS16570"
                     /old_locus_tag="pgaptmp_003314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic amino acid transporter AroP"
                     /protein_id="HGU4549205.1"
                     /translation="MMEGQQHGEQLKRGLKNRHIQLIALGGAIGTGLFLGSASVIQSA
                     GPGIILGYAIAGFIAFLIMRQLGEMVVEEPVAGSFSHFAYKYWGSFAGFASGWNYWVL
                     YVLVAMAELTAVGKYIQFWYPEIPTWVSAAVFFVVINAINLTNVKVFGEMEFWFAIIK
                     VIAVVAMIIFGGWLLFSGNGGPQATVSNLWDQGGFLPHGFTGLVMMMAMIMFSFGGLE
                     LVGITAAEADNPEQSIPKATNQVIYRILIFYIGSLAVLLSLMPWTRVTADTSPFVLIF
                     HELGDTFVANALNIVVLTAALSVYNSCVYCNSRMLFGLAQQGNAPKALASVDKRGVPV
                     NTILVSALVTALCVLINYLAPESAFGLLMALVVSALVINWAMISLAHMKFRRAKQEQG
                     VVTRFPALLYPLGNWICLLFMAAVLVIMLMTPGMAISVYLIPVWLIVLGIGYLFKEKT
                     AKAVKAH"
     gene            complement(1631..2485)
                     /gene="ampE"
                     /locus_tag="ACHOSC_RS16575"
                     /old_locus_tag="pgaptmp_003315"
     CDS             complement(1631..2485)
                     /gene="ampE"
                     /locus_tag="ACHOSC_RS16575"
                     /old_locus_tag="pgaptmp_003315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-lactamase regulator AmpE"
                     /protein_id="HGU4549206.1"
                     /translation="MTLFTTLLVLIFERLFKLGEHWQLDHRLEAFFRRVKHFSLGRTL
                     GMTIIAMGVTFLLLRALQGVLFNVPTLLVWLLIGLLCIGAGKVRLHYHAYLTAASRND
                     SHARATMAGELTMIHGVPAGCDEREYLRELQNALLWINFRFYLAPLFWLIVGGTWGPV
                     TLMGYAFLRAWQYWLARYQTPHHRLQSGIDAVLHVLDWVPVRLAGVVYALIGHGEKAL
                     PAWFASLGDFHTSQYQVLTRLAQFSLAREPHVDKVETPRAAVSMAKKTSFVVVVVIAL
                     LTIYGALV"
     gene            complement(2482..3033)
                     /gene="ampD"
                     /locus_tag="ACHOSC_RS16580"
                     /old_locus_tag="pgaptmp_003316"
     CDS             complement(2482..3033)
                     /gene="ampD"
                     /locus_tag="ACHOSC_RS16580"
                     /old_locus_tag="pgaptmp_003316"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008745 - N-acetylmuramoyl-L-alanine
                     amidase activity [Evidence IEA];
                     GO_process: GO:0009253 - peptidoglycan catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1,6-anhydro-N-acetylmuramyl-L-alanine amidase
                     AmpD"
                     /protein_id="HGU4549207.1"
                     /translation="MLLEQGWLVGARRVPSPHYDCRPDDETPTLLVVHNISLPPGEFG
                     GPWIDALFTGTIDPQAHPFFAEIAHLRVSAHCLIRRDGEIVQYVPFDKRAWHAGVSQY
                     QGRERCNDFSIGIELEGTDTLAYTDAQYQQLAAVTRALIDCYPDIAKNMTGHCDIAPD
                     RKTDPGPAFDWARFRVLVSKETT"
     gene            3121..4014
                     /gene="nadC"
                     /locus_tag="ACHOSC_RS16585"
                     /old_locus_tag="pgaptmp_003317"
     CDS             3121..4014
                     /gene="nadC"
                     /locus_tag="ACHOSC_RS16585"
                     /old_locus_tag="pgaptmp_003317"
                     /EC_number="2.4.2.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004514 - nicotinate-nucleotide
                     diphosphorylase (carboxylating) activity [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxylating nicotinate-nucleotide
                     diphosphorylase"
                     /protein_id="HGU4549208.1"
                     /translation="MPPRRYNPDTRRDELLERINLDIPGAVAQALREDLGGTVDANND
                     ITAKLLPENSRSHATVITRENGVFCGKRWVEEVFIQLAGDDVTIIWHVDDGDVINANQ
                     SLFELEGPSRVLLTGERTALNFVQTLSGVASKVRHYVELLEGTNTQLLDTRKTLPGLR
                     SALKYAVLCGGGANHRLGLSDAFLIKENHIIASGSVRQAVEKASWLHPDAPVEVEVEN
                     LEELDEALKAGADIIMLDNFETEQMREAVKRTNGKALLEVSGNVTDKTLREFAETGVD
                     FISVGALTKHVQALDLSMRFR"
     gene            4217..4657
                     /gene="ppdD"
                     /locus_tag="ACHOSC_RS16590"
                     /old_locus_tag="pgaptmp_003318"
     CDS             4217..4657
                     /gene="ppdD"
                     /locus_tag="ACHOSC_RS16590"
                     /old_locus_tag="pgaptmp_003318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase-dependent pilin"
                     /protein_id="HGU4549209.1"
                     /translation="MDKQRGFTLIELMVVIGIIAILSAIGIPAYQNYLRKAALTDMLQ
                     TFVPYRTAVELCALEHGGLDTCDGGSNGIPSPTTTRYVSAMSVAKGVVSLTGQESLNG
                     LSVVMTPGWDNANGVTGWTRNCNIQSDSALHQACEDVFRFDDAN"
     gene            4667..6052
                     /gene="gspE"
                     /locus_tag="ACHOSC_RS16595"
                     /old_locus_tag="pgaptmp_003319"
     CDS             4667..6052
                     /gene="gspE"
                     /locus_tag="ACHOSC_RS16595"
                     /old_locus_tag="pgaptmp_003319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein GspE"
                     /protein_id="HGU4549210.1"
                     /translation="MNIPQLTALCLRYHGVLLDASEEVVHVAVVDAPSHELLDALHFA
                     TTKRIEITCWTRQQMEGHASRTQQTLPVAVQEKHQPKAELLTRTLQSALEQRASDIHI
                     EPADNAYRIRLRIDGVLHPLPDVSPDAGVALTARLKVLGNLDIAEHRLPQDGQFTVEL
                     AGNAVSFRIATLPCRGGEKVVLRLLQQVSQALDVNTLGMQPLQLADFAHALQQPQGLV
                     LVTGPTGSGKTVTLYSALQTLNTADINICSVEDPVEIPIAGLNQTQIHPRAGLTFQGV
                     LRALLRQDPDVIMIGEIRDGETAEIAIKAAQTGHLVLSTLHTNSTCETLVRLQQMGVA
                     RWMLSSALTLVIAQRLVRKLCPHCRRQQGEPIHIPDNVWPSPLPHWQAPGCVHCYHGF
                     YGRTALFEVLPITPVIRQLISANTDVESLETHARQAGMRTLFENGCLAVEQGLTTFEE
                     LIRVLGMPHGE"
     gene            6042..7244
                     /gene="hofC"
                     /locus_tag="ACHOSC_RS16600"
                     /old_locus_tag="pgaptmp_003320"
     CDS             6042..7244
                     /gene="hofC"
                     /locus_tag="ACHOSC_RS16600"
                     /old_locus_tag="pgaptmp_003320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004827 - proline-tRNA ligase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein transport protein HofC"
                     /protein_id="HGU4549211.1"
                     /translation="MASKQLWRWHGITGDGNAQDGMLWAESRALLLMALQQQMVTPLS
                     LKRIAINSAQWRGDKSAEVIHQLATLLKAGLTLSEGLALLAEQHPSKQWQALLQSLAH
                     DLEQGIAFSNALLPWSEVFPPLYQAMIRTGELTGKLDECCFELARQQKAQRQLTDKVK
                     SALRYPIIILAMAIMVVVAMLHFVLPEFAAIYKTFNTPLPALTQGIMTLADFSGEWSW
                     LLVLFGFLLAIANKLLMRRPTWLIVRQKLLLRIPIMGSLMRGQKLTQIFTILALTQSA
                     GITFLQGVESVRETMRCPYWVQLLTQIQHDISNGHPIWLALKNTGEFSPLCLQLVRTG
                     EASGSLDLMLDNLAHHHRDNTMALADNLAALLEPALLIITGGIIGTLVVAMYLPIFHL
                     GDAMSGMG"
     gene            complement(7279..8322)
                     /gene="guaC"
                     /locus_tag="ACHOSC_RS16605"
                     /old_locus_tag="pgaptmp_003321"
     CDS             complement(7279..8322)
                     /gene="guaC"
                     /locus_tag="ACHOSC_RS16605"
                     /old_locus_tag="pgaptmp_003321"
                     /EC_number="1.7.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008461816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902560 - GMP reductase complex [Evidence
                     IEA];
                     GO_function: GO:0003920 - GMP reductase activity [Evidence
                     IEA];
                     GO_process: GO:0009117 - nucleotide metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GMP reductase"
                     /protein_id="HGU4549212.1"
                     /translation="MRIEEDLKLGFKDVLIRPKRSTLKSRSDVELERQFTFKHSGQSW
                     SGVPIIAANMDTVGTFSMASALASFDILTAVHKHYSVEEWQAFINNSSADVLKHVMVS
                     TGTSDADFEKTKQILDLNPALNFVCIDVANGYSEHFVQFVAKAREAWPTKTICAGNVV
                     TGEMCEELILSGADIVKVGIGPGSVCTTRVKTGVGYPQLSAVIECADAAHGLGGMIVS
                     DGGCTTPGDVAKAFGGGADFVMLGGMLAGHEESGGRIVEENGEKFMLFYGMSSESAMK
                     RHVGGVAEYRAAEGKTVKLPLRGPVENTARDILGGLRSACTYVGASRLKELTKRTTFI
                     RVQEQENRIFNNL"
     gene            complement(8478..8522)
                     /locus_tag="ACHOSC_RS16610"
                     /old_locus_tag="pgaptmp_003322"
     CDS             complement(8478..8522)
                     /locus_tag="ACHOSC_RS16610"
                     /old_locus_tag="pgaptmp_003322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4549213.1"
                     /translation="MIIKTLKMSARKRN"
     gene            8547..9167
                     /gene="coaE"
                     /locus_tag="ACHOSC_RS16615"
                     /old_locus_tag="pgaptmp_003323"
     CDS             8547..9167
                     /gene="coaE"
                     /locus_tag="ACHOSC_RS16615"
                     /old_locus_tag="pgaptmp_003323"
                     /EC_number="2.7.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308134.1"
                     /note="Dephospho-CoA kinase (CoaE) performs the final step
                     in coenzyme A biosynthesis; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004140 - dephospho-CoA kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dephospho-CoA kinase"
                     /protein_id="HGU4549214.1"
                     /translation="MRYIVALTGGIGSGKSTVANAFADLGINVIDADIIARQVVEPGA
                     PALHAIADHFGANMIAADGTLQRRALRERIFANPEEKNWLNALLHPLIQQETQHQIQQ
                     ATSPYVLWVVPLLVENSLYKKANRVLVVDVSPETQLKRTMQRDDVTREHVEQILAAQA
                     TREARLAVADDVIDNNGAPDAIASDVARLHAHYLQLASQFVSQEKP"
     gene            9167..9910
                     /gene="zapD"
                     /locus_tag="ACHOSC_RS16620"
                     /old_locus_tag="pgaptmp_003324"
     CDS             9167..9910
                     /gene="zapD"
                     /locus_tag="ACHOSC_RS16620"
                     /old_locus_tag="pgaptmp_003324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapD"
                     /protein_id="HGU4549215.1"
                     /translation="MQTQVLFEHPLNEKMRTWLRIEFLIQQLTVNLPIVDHAGALHFF
                     RNVSELLDVFERGEVRTELLKELDRQQRKLQTWIGVPGVDQSRIEALIQQLKAAGSVL
                     ISAPRIGQFLREDRLIALVRQRLSIPGGCCSFDLPTLHIWLHLPQAQRDSQVETWIAS
                     LNPLTQALTMVLDLIRQSAPFRKQTSLNGFYQDNGGDADLLRLNLSLDSQLYPQISGH
                     KSRFAIRFMPLDTENGQVPERLDFELACC"
     gene            9920..10117
                     /gene="yacG"
                     /locus_tag="ACHOSC_RS16625"
                     /old_locus_tag="pgaptmp_003325"
     CDS             9920..10117
                     /gene="yacG"
                     /locus_tag="ACHOSC_RS16625"
                     /old_locus_tag="pgaptmp_003325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase inhibitor YacG"
                     /protein_id="HGU4549216.1"
                     /translation="MSETITVNCPTCGKTVVWGEISPFRPFCSKRCQLIDLGEWAAEE
                     KRIPSSGDLSESDDWSEEPKQ"
BASE COUNT         2488 a         2668 c         2596 g         2381 t
ORIGIN      
        1 gctttacaaa cggtttcttt ttaagcaact catcttcaac catgcataaa gcgggtgcat
       61 tcgctgcctc ataccattat tcttgatctg acggaagtct ttttgtaaca attcaaactt
      121 ctttgatgta aacaaattaa tacaacaaac ggaattgcaa acttacacac gcatcactgc
      181 gtagatcaaa aaaacaacca ccgcacgagg tttcatgatg gaaggtcaac agcacggcga
      241 gcagctaaag cgcggcctta aaaaccgcca tattcagctt atcgcgctgg gtggcgcgat
      301 agggaccggg ttattcctgg gtagcgcctc cgtaatacag tccgcagggc cagggattat
      361 cctgggttac gccattgctg gttttatcgc ctttctgatc atgcgtcagc tgggtgaaat
      421 ggtggtcgaa gaacctgtcg caggctcctt tagccacttt gcttataaat actggggcag
      481 ttttgccggt ttcgcctctg gctggaacta ctgggtactg tacgttttag ttgccatggc
      541 tgagctgact gccgtgggta aatacattca gttctggtat ccggaaatcc ccacctgggt
      601 ttctgccgcc gtattctttg tggtgattaa cgccatcaac ctgaccaacg ttaaagtgtt
      661 tggcgagatg gagttctggt ttgccattat caaagttatc gcggtggtag cgatgatcat
      721 cttcggcggc tggctgctat tcagtggcaa cggcggcccg caggcgaccg ttagcaacct
      781 gtgggatcag ggtggtttcc tgccgcacgg cttcaccggg ctggtgatga tgatggcgat
      841 gatcatgttc tcgttcggtg gtctggaact ggtggggatc accgcagcag aagctgataa
      901 cccggagcaa agtataccga aagcaactaa ccaggttatc taccgcatcc tgattttcta
      961 tattggttcg ttagccgttc tgctctcact gatgccgtgg acccgcgtta ccgccgatac
     1021 cagtccgttt gtgctgatct tccacgagtt aggcgatacc tttgtggcga atgcgctgaa
     1081 catcgtggta ctgactgcgg cgctctccgt gtacaacagc tgcgtatatt gcaacagccg
     1141 tatgctgttt ggtctggcac aacagggtaa tgcgccaaaa gcgctggcgt ctgtcgataa
     1201 acgtggtgta ccagtaaata ccattctggt gtctgcactg gtaacggcgt tgtgcgtact
     1261 gattaactac cttgccccag agtccgcttt cggactgtta atggcgctgg tggtatctgc
     1321 actggtaatc aactgggcga tgattagcct ggcgcatatg aaattccgtc gcgccaagca
     1381 ggaacaaggc gtggtaactc gcttccctgc tctgctttat ccgctgggta actggatctg
     1441 cctgctgttt atggcggcgg tactggtgat tatgctgatg accccaggaa tggcgatttc
     1501 ggtatacctg atcccggtat ggctgatcgt gttaggtatc ggctatctgt ttaaagagaa
     1561 aaccgccaaa gccgtaaaag cgcattaatc tctctacgcc ctcacccgta cagggtgagg
     1621 gcaataatct ttacaccaac gccccgtaaa tcgtcagtag tgcaatcacc accacgacca
     1681 cgaacgaggt tttcttcgcc attgaaaccg ctgccctcgg cgtctccacc ttatcgacat
     1741 gcggttcacg cgccagagag aactgcgcca gacgcgttaa cacctgatac tgcgaagtat
     1801 ggaaatcacc cagcgaagca aaccaggccg gtaacgcttt ctcaccatga ccgatcaagg
     1861 catataccac acccgcaaga cgaaccggca cccaatccag tacatgaagc acggcatcaa
     1921 tgccggactg taaacgatga tgcggcgtct gatatcgcgc cagccagtat tgccatgcac
     1981 gcaaaaacgc ataccccatc agcgtaacgg gtccccaggt tccccccaca atcagccaga
     2041 acagcggtgc aagataaaaa cgaaagttaa tccacagcaa tgcattttgc agctcacgca
     2101 aatactcacg ttcgtcgcag cctgccggga cgccgtgaat catggtgagt tcgccagcca
     2161 tcgtggcacg ggcatggcta tcattacgtg aagcagctgt cagataagca tgataatgaa
     2221 gacgaacttt acctgcgcca atacacagca aaccaatcag cagccacacc agtagcgtgg
     2281 gaacgttgaa caatactccc tgcaatgcgc gtaacagtaa aaaagtcacg cccatcgcaa
     2341 taatggtcat gcctaacgtg cgcccgagag aaaaatgttt cacccgccga aagaacgctt
     2401 caagacgatg atcaagctgc cagtgctcgc ccaacttaaa caggcgctcg aaaattaaca
     2461 ccagtaaggt tgtaaatagc gtcatgttgt ctccttgctg accagcacac gaaaccgtgc
     2521 ccaatcaaat gcaggaccgg gatcggtttt ccgatccggc gcaatatcac aatggcccgt
     2581 catgttttta gcgatatccg gatagcaatc aatcagtgcc cgcgtaaccg ccgcaagctg
     2641 ttgatactgc gcatcggtat acgccagcgt atcggtgcct tcaagctcaa tcccaataga
     2701 aaaatcattg cagcgttcgc gcccctgata ctgagagact cccgcatgcc atgcacgttt
     2761 atcgaaagga acatactgga ctatttcacc atcacggcga atcaaacagt gagcggagac
     2821 gcgcaaatgg gcgatctcag caaagaaagg atgtgcctgc ggatcaatag ttccagtgaa
     2881 taatgcgtcg atccacggac cgccaaactc gcctggcggc aggctaatat tgtgcaccac
     2941 cagcagggtg ggtgtttcgt catccgggcg gcaatcgtaa tgtggtgagg gaacgcggcg
     3001 cgcgccaacc agccacccct gttctaacaa catgcaggat ctccttatat gtggtgctaa
     3061 tacccggttc agagtagcat gtttctacct tatgattcgt tagctatctg gagttttaac
     3121 atgccgcctc gccgctataa ccctgacacc cgacgtgacg agctgctgga acgcattaat
     3181 ctcgatatcc ccggcgcggt agcccaggcg ctgcgggaag atttaggcgg aacagtcgat
     3241 gccaacaatg atattacggc aaaactttta ccggaaaatt ctcgctctca tgccacggtg
     3301 atcacccgcg agaatggcgt cttttgcggc aaacgctggg ttgaagaggt gtttattcaa
     3361 ctggcaggcg acgatgtcac cataatctgg catgtggatg acggcgatgt catcaatgcc
     3421 aatcaatcct tgttcgaact tgaaggccca tcccgcgtgc tgttaacggg cgaacgcact
     3481 gcgcttaatt ttgtgcaaac cctttcagga gttgccagta aggtacgcca ctatgtcgaa
     3541 ttgctggaag gcaccaacac gcagttgttg gatacgcgca aaaccttacc cggcctgcgt
     3601 tcagctctga aatacgcggt actttgcggc ggcggagcga atcaccgtct ggggctttct
     3661 gatgccttcc tgatcaaaga aaaccatatt attgcctccg gctcagtgcg ccaggcggtc
     3721 gaaaaagcgt cctggctgca cccggatgcg ccagtagaag tcgaagtaga gaatctggaa
     3781 gaacttgatg aagccctgaa agcaggagcc gatatcatca tgctggataa cttcgaaaca
     3841 gaacagatgc gcgaagccgt caaacgcacc aacggcaagg cgctactgga agtgtctggc
     3901 aacgtcactg acaaaacact gcgtgaattt gccgaaacgg gcgtggactt tatctccgtc
     3961 ggtgcgctaa ctaaacacgt acaagcactc gacctttcaa tgcgttttcg ctaatagttg
     4021 acagatttat ccgctccatc gcgggcggat aatctcccct tccccaactt tcttcgtaac
     4081 gcctcgcaaa tttcttgtct ttcgctgcaa cgaaataaaa aacctcggaa tctgctttcc
     4141 cgtttcgttt tttgcccctc gcctgtgctg atggcgtttg ccaaagtagc gccaactaaa
     4201 tcaaggagcg aaacagatgg acaagcaacg cggttttaca cttatcgaac tgatggtggt
     4261 tattggcatc attgccattt taagcgccat tggtattccc gcttatcaaa actacctgcg
     4321 caaagccgca ctcaccgaca tgctacaaac ctttgtgcct taccgtaccg ccgtagagtt
     4381 gtgcgcgctg gaacatggtg gattagatac ctgcgacggt ggcagcaatg gcattccctc
     4441 gcctaccacc acccgctatg tttcagccat gagtgtggca aagggcgtgg tgtcgctgac
     4501 cgggcaagaa agtctcaatg ggctaagcgt cgtcatgaca ccgggttggg ataacgcaaa
     4561 cggcgtcacc ggctggacgc gcaactgcaa tattcaaagt gacagcgcat tgcatcaagc
     4621 ctgcgaagat gtcttccgct ttgatgacgc caactaagga gcggcaatga atattccaca
     4681 gctcactgcc ctgtgtctgc gttatcatgg agtcttgctg gatgccagcg aagaggtggt
     4741 tcatgttgcg gtagtcgatg caccttcgca tgagctactg gacgcattgc atttcgctac
     4801 caccaaacgt attgagatca cctgctggac gcgccaacaa atggaaggtc acgccagtcg
     4861 cacacaacag acattgcccg tagctgttca ggagaagcat cagcccaaag cagagttgct
     4921 aactcgaacg ttacaatctg cgctggaaca acgcgcgtct gatattcata tcgaaccagc
     4981 ggacaatgcc taccgcatcc gcttgcgtat cgacggcgta ttgcatcctt taccggatgt
     5041 ttcaccggat gccggagtcg cattaaccgc cagattaaaa gtgctgggaa acctggatat
     5101 tgcggaacat cgcctgccgc aggacgggca attcactgtc gaactggcag gaaacgccgt
     5161 ctcatttcgt attgcgacct taccatgtcg gggtggtgaa aaggtggtat taaggttgtt
     5221 acagcaggtg agccaggcac tggatgtcaa cacgcttgga atgcagccgt tacaactggc
     5281 ggactttgct catgccttgc aacaaccaca gggactggtg ctggtaactg gccctacagg
     5341 cagcggcaaa acggtcacgc tttatagtgc cctgcaaacg ctgaataccg ctgacattaa
     5401 tatttgtagc gtcgaagatc cggttgagat ccccatagcc ggactaaacc agacgcaaat
     5461 ccatccgcgt gccggactca cctttcaggg cgtgttgcgt gcgttattgc gccaggatcc
     5521 tgacgtcatc atgatcggag agatccgcga tggcgaaaca gcagagatcg ctattaaagc
     5581 ggcgcaaact ggtcacctgg tgttgtctac cctacacact aattccacct gcgaaacgct
     5641 ggtacgttta cagcaaatgg gggtcgcccg ctggatgcta tcatcggcgc ttacgctggt
     5701 aatagcccag cgtctggtac gcaaactttg cccacattgt cgccggcagc aaggggagcc
     5761 catccacatt ccagacaatg tatggccatc gccgctgccc cactggcagg cacccggttg
     5821 tgtacattgc taccacggtt tttatggtcg tacggcctta tttgaagttc tgcccataac
     5881 gccggtcatt cgtcagctta tttccgctaa taccgacgtt gaatcgctgg aaacgcacgc
     5941 acgacaggcg ggtatgcgta cgctttttga aaacggctgc ctggccgtgg agcaaggctt
     6001 aaccaccttt gaagagttaa tccgcgtact ggggatgccg catggcgagt aagcaactct
     6061 ggcgctggca tggcataacc ggcgacggca atgcgcaaga tgggatgcta tgggcagaga
     6121 gccgtgcttt gctgctcatg gcactacagc aacagatggt taccccactt agcctgaagc
     6181 gaatcgccat caattctgcg cagtggcgag gagataaaag cgcggaagtc attcatcaac
     6241 tggcgacgct actcaaagcc gggttaacgc tttctgaagg gctggcactg ctggcggaac
     6301 agcatcccag taagcaatgg caagcgttgc tgcaatcgct ggcgcacgat ctcgaacagg
     6361 gcattgcttt ttccaatgcc ttattaccct ggtcagaggt atttccgcca ctctatcagg
     6421 cgatgatccg cacgggtgaa ctgaccggta agctggatga atgctgcttt gaactggcgc
     6481 gtcagcaaaa agcccagcgt cagttgaccg acaaagtgaa atcagcgtta cgttatccca
     6541 tcatcatttt agcgatggca atcatggtgg ttgtggcaat gctgcatttt gttctgccgg
     6601 agtttgccgc tatctataag accttcaaca ccccactacc ggcactaacg caggggatca
     6661 tgacgctggc agactttagt ggcgaatgga gctggctgct ggtgttgttc ggctttctgc
     6721 tggcgatagc caataagttg ctgatgcgcc gaccgacctg gcttatagtg cggcagaaat
     6781 tgctgttacg catcccgatt atgggttcac tgatgcgggg acaaaaactc acgcagattt
     6841 ttacgattct ggcgctgaca caaagtgcag gcattacttt tttgcagggc gtagagagcg
     6901 tcagagaaac aatgcgctgc ccgtactggg tgcaacttct gacacaaatc cagcacgata
     6961 tcagtaacgg tcatcccatc tggctggcgc taaaaaatac cggggagttt agcccgctct
     7021 gtttgcaatt agtgagaaca ggagaggcat ccggctcgct ggacctcatg ttagacaacc
     7081 tcgcccatca tcatcgggat aacacaatgg cgctggcgga taacctcgca gccttactgg
     7141 aaccggcgtt gctgatcata acgggaggaa ttatcggtac gctggtggtg gcgatgtatc
     7201 tgccaatttt ccatttaggc gatgcgatga gtgggatggg ataacgctgg cgtgttgctc
     7261 cacaccagcg ttggaagatt acaggttgtt gaagatgcgg ttttcttgtt cctgcacacg
     7321 gataaatgtg gtgcgcttgg tcagttcttt caggcgtgaa gccccaacgt atgtacaagc
     7381 tgaacgcagg ccgcccaaaa tatcacgcgc ggtattttca accgggcctc gcagcggcag
     7441 cttaacggtt ttaccttctg ctgcgcgata ttccgcaacg ccgccaacgt gacgtttcat
     7501 cgcagactcg gagctcatgc cgtagaacag cataaatttc tcgccgttct cctcaacgat
     7561 gcgaccgccg ctctcttcgt ggcccgccag catgccgcca agcatgacga aatcggcacc
     7621 gccgccaaag gcttttgcga catcgcccgg cgtggtgcag ccaccgtcgc tgacgatcat
     7681 tccgcccaga ccgtgcgccg catcggcaca ttcgattacc gcagaaagct gcggataacc
     7741 gacgcctgtt ttgacgcgag ttgtacaaac agaacctggg ccaatgccaa ctttaacgat
     7801 atcagcaccg gagaggataa gctcctcaca catttcacca gtcactacgt tgccagcaca
     7861 aatggttttg gtcggccacg cttcacgcgc tttcgcaacg aactgcacga agtgttcgga
     7921 ataaccattc gccacatcaa tacaaacgaa gtttaatgcc gggttcaggt cgagaatctg
     7981 tttagttttt tcgaaatccg catcagacgt accggtagaa accatcacat gtttcagcac
     8041 atcagcggaa gaattgttga taaacgcttg ccactcttcg acagaatagt gtttatgcac
     8101 agcagtcaaa atatcaaaag aagccagcgc agaggccata gaaaatgtac ctacggtgtc
     8161 catatttgcg gcgataattg gcacgccgga ccagctctga cctgaatgtt tgaaggtgaa
     8221 ttgacgttcc agttcaacat cggaacggct tttaagagtg gagcgtttag ggcggatgag
     8281 aacgtcttta aaacctaact tcagatcttc ttcaatacgc atgtgcggat tcctggggtt
     8341 aatggctata agggtaagag cgcaactcca gtgacgctat catacgcact aatcaatgcg
     8401 gcgcaagact gcgaaattgt ctttttttac gctacaatcc cataaattta cctggtaaat
     8461 agtgagcgaa cgtttcatca atttcgcttt cgtgcactca tttttaacgt tttgataatc
     8521 atgatgaatt cccgggaata taaattatga ggtatatagt tgccttaacg ggaggcattg
     8581 gcagtggcaa gagtaccgtt gccaatgcgt ttgctgatct cggaattaac gtcattgatg
     8641 ccgatattat tgcgcgtcag gtggttgaac caggtgcacc tgcgctacat gccattgctg
     8701 atcactttgg cgctaacatg attgctgctg atggaacatt gcagcgccgg gccttgcgcg
     8761 agcggatctt cgccaacccg gaagagaaaa actggcttaa cgccctgctg catccgctga
     8821 ttcagcaaga gacgcaacac cagatccagc aagctacttc cccctatgta ctgtgggttg
     8881 tgccattgct ggtagaaaac tcactgtata aaaaagcgaa tcgagtgctg gtggtggatg
     8941 tcagcccaga aacgcaactt aagcgcacca tgcagcgcga tgatgtaact cgcgagcatg
     9001 tcgaacaaat ccttgctgct caggcaacgc gcgaagcccg ccttgccgtg gcagatgacg
     9061 tcattgataa taacggcgca ccggatgcta tcgcatcgga tgttgcccgc ctgcacgcac
     9121 actatttgca gcttgcgtcg cagtttgtct cacaggaaaa accgtaatgc agacccaggt
     9181 cctttttgaa catccactaa atgaaaaaat gcgtacatgg ctgcgcattg agtttttgat
     9241 tcagcaactc accgttaatt tacccatcgt tgaccacgct ggcgcgctgc atttcttccg
     9301 taatgtcagt gaattactgg atgttttcga gcgcggcgaa gtccgcactg agctgttgaa
     9361 agaacttgac cggcagcaac gtaaactcca gacctggatt ggcgtgcctg gcgtggacca
     9421 gagccgtatt gaagcattaa ttcagcagtt aaaagcggcg gggagcgtat taatttccgc
     9481 gccgcgtatc gggcaatttc tgcgtgaaga tcgtttgatt gctctggtgc gtcagcgact
     9541 gagcatccca ggcggctgtt gcagctttga tttacctaca ttgcacattt ggctgcatct
     9601 accccaggcg cagcgcgaca gccaggtaga aacctggatt gccagcctga acccgctcac
     9661 ccaggcactt accatggtgc tggatttaat tcgccagtcg gctcccttcc gtaaacaaac
     9721 cagcctgaat ggtttttatc aggataacgg tggcgatgcc gacttgctgc gcctgaatct
     9781 gtcgctcgat tcacagcttt atccgcaaat ttccggacat aagagccgtt ttgccattcg
     9841 ttttatgccg ctggacactg aaaacggaca ggtaccggaa cgtctggatt tcgaactggc
     9901 ctgttgctaa ggagtgaaaa tgtcagaaac tattacggtg aattgcccaa cctgcgggaa
     9961 aacggtggtg tggggtgaaa tcagcccgtt tcggccattt tgctccaaac gttgtcagct
    10021 gatcgacctc ggagaatggg ctgctgaaga aaaacgaatc cccagcagtg gcgatctttc
    10081 cgaaagcgat gactggagcg aagagccaaa gcagtgacat ttgtctgatg ccg
//
LOCUS       DAZKHF020000167        10083 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2201S31241
            SAMN43267800-rid25106413.denovo.168, whole genome shotgun sequence.
ACCESSION   DAZKHF020000167 DAZKHF020000000
VERSION     DAZKHF020000167.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267800
            Sequence Read Archive: SRR30307263
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 10083)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10083)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 10083)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350581.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350581.2
            Genome Coverage       :: 151x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:21:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,652
            CDSs (total)                      :: 5,557
            Genes (coding)                    :: 5,320
            CDSs (with protein)               :: 5,320
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 237
            CDSs (without protein)            :: 237
            Pseudo Genes (ambiguous residues) :: 0 of 237
            Pseudo Genes (frameshifted)       :: 102 of 237
            Pseudo Genes (incomplete)         :: 130 of 237
            Pseudo Genes (internal stop)      :: 52 of 237
            Pseudo Genes (multiple problems)  :: 41 of 237
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10083
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267800-rid25106413.denovo.168"
                     /serotype="O 26:H11"
                     /isolate="2201S31241"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-01"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            complement(<1..278)
                     /locus_tag="ACHOSC_RS16630"
                     /old_locus_tag="pgaptmp_003326"
     CDS             complement(<1..278)
                     /locus_tag="ACHOSC_RS16630"
                     /old_locus_tag="pgaptmp_003326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TerB family tellurite resistance protein"
                     /protein_id="HGU4549217.1"
                     /translation="MFGIFKKKTRRAAAEIKKFEKRDLAQAVINAAYLVAYADGECEA
                     SEKAKIEQVLRNQPALSAFTSEINAISATIIGQLDTNFKIGRRAALRE"
     gene            complement(448..524)
                     /locus_tag="ACHOSC_RS16635"
                     /old_locus_tag="pgaptmp_003327"
     tRNA            complement(448..524)
                     /locus_tag="ACHOSC_RS16635"
                     /old_locus_tag="pgaptmp_003327"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(488..490),aa:Arg,seq:tct)
     gene            complement(538..614)
                     /locus_tag="ACHOSC_RS16640"
                     /old_locus_tag="pgaptmp_003328"
     tRNA            complement(538..614)
                     /locus_tag="ACHOSC_RS16640"
                     /old_locus_tag="pgaptmp_003328"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(578..580),aa:Arg,seq:tcg)
     gene            complement(624..699)
                     /locus_tag="ACHOSC_RS16645"
                     /old_locus_tag="pgaptmp_003329"
     tRNA            complement(624..699)
                     /locus_tag="ACHOSC_RS16645"
                     /old_locus_tag="pgaptmp_003329"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(664..666),aa:Ile,seq:cat)
     gene            complement(749..1798)
                     /locus_tag="ACHOSC_RS16650"
                     /old_locus_tag="pgaptmp_003330"
     CDS             complement(749..1798)
                     /locus_tag="ACHOSC_RS16650"
                     /old_locus_tag="pgaptmp_003330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001575625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific DNA-methyltransferase"
                     /protein_id="HGU4549218.1"
                     /translation="MFNTVKISSCELINADCLEFIRSLPENSVDLIVTDPPYFKVKPE
                     GWDNQWTGDEDYLKWLDQCLAQFWRVLKPAGSLYLFFGHRLASDTEIMMRERFNVLNH
                     IIWAKPSGRWNGCNKESLRAYFPATERILFAEHYQGPYRPKDDGYVAQGRELKQHVMA
                     PLISYFRDARKSLGITSKQIAEATGKKNMASHWFGTSQWQLPNEGNYNKLQALFARVA
                     AEKHQRGELEKPHHQLVSTYSGLNRQYTELLSEYKNLRRYFGVTAQVPYTDVWTHKPV
                     QYYPGKHPCEKPAEMLQQIINASSRPGDLVADFFMGSGSTVKAAMALGRCAIGVELET
                     GRFEQTVREVQDLIV"
     gene            complement(1950..2147)
                     /locus_tag="ACHOSC_RS16655"
                     /old_locus_tag="pgaptmp_003331"
     CDS             complement(1950..2147)
                     /locus_tag="ACHOSC_RS16655"
                     /old_locus_tag="pgaptmp_003331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309806.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TrmB family transcriptional regulator"
                     /protein_id="HGU4549219.1"
                     /translation="MLKQQDMTETARVVFNELSATAPATVGEIAQNTYLSRERCQLIL
                     TQLVMAGLADYQFGCYRRLQS"
     gene            complement(2363..2743)
                     /locus_tag="ACHOSC_RS16660"
                     /old_locus_tag="pgaptmp_003332"
     CDS             complement(2363..2743)
                     /locus_tag="ACHOSC_RS16660"
                     /old_locus_tag="pgaptmp_003332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001204744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0060567 - negative regulation of
                     termination of DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="antiterminator Q family protein"
                     /protein_id="HGU4549220.1"
                     /translation="MRDIQMVLERWGAWAASGNTGVDYSPIAAGFKGLLPSTTKPRPA
                     CCDDDGLIIENCLARLKQKKPEEYSLLIAHYLLRISKRQIARTRKKSEKAIRIEMQIA
                     EGFIDGCLSVLGVRLEMDDWLLKK"
     gene            complement(2762..3751)
                     /locus_tag="ACHOSC_RS16665"
                     /old_locus_tag="pgaptmp_003333"
     CDS             complement(2762..3751)
                     /locus_tag="ACHOSC_RS16665"
                     /old_locus_tag="pgaptmp_003333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001764249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF968 domain-containing protein"
                     /protein_id="HGU4549221.1"
                     /translation="MRALLTPEIAPRMGIVLFRPGSELMPLFMQGRVLLEPEPERYSS
                     FASGAVPAASQPLADDPAVRAVFRHEAVIRRAGGVECLESWLLREKGCQWPHSGWHSE
                     NMTTMRHAPGAIRLCWHCDNLLRDQFTERLESMATDNCARWVLSVVRRDLGFDDSHVV
                     TMPELCWWLVRNDLADALPESAARKALRLPKPVLPSVTRESDLVPSVPATSIIQDKAK
                     KVLALKVDPESPESFMLRPKRRRWVNEKYTRWVKTQPCACCGKPADDPHHLIGHGQGG
                     MGTKAHDLFVLPLCRKHHDELHADTVAFEEKYGSQLELIFRFIDRALAIGVLS"
     gene            complement(3803..4060)
                     /locus_tag="ACHOSC_RS16670"
                     /old_locus_tag="pgaptmp_003334"
     CDS             complement(3803..4060)
                     /locus_tag="ACHOSC_RS16670"
                     /old_locus_tag="pgaptmp_003334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009453440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4549222.1"
                     /translation="MNNRTTTVSPEQLRRQAQEMLRCAEQMEKTSVAKDTLRKQLTPA
                     LRDLLQAKHRTQKAVDELVDCVAELEGQVSQFETLVKEFTA"
     gene            complement(4057..5457)
                     /locus_tag="ACHOSC_RS16675"
                     /old_locus_tag="pgaptmp_003335"
     CDS             complement(4057..5457)
                     /locus_tag="ACHOSC_RS16675"
                     /old_locus_tag="pgaptmp_003335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000203847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replicative DNA helicase"
                     /protein_id="HGU4549223.1"
                     /translation="MTSQNTPAWRNDDLEGAVIGAFFLRGADPEVMDILATLSADVFS
                     ARQYRDIYAGICRQARVSGVIDPVLLCNEMPELAPVITDTGRKTWVKSSLEHYVAALR
                     RNAALRDAEKTLTEALQNLRDAHTCEAAEDALKDAQNMMASLSTGKGVIQPVHIDDVL
                     PEVVDRVECRNQGLEKSRALMTGIDELDAKTGGMEPGDLVFIAARPSMGKTELALDII
                     DKVTEQGHGVLLFTMEMANIQIGERMVSAAGGMPVSRLKSVARFEDEDWARFSQGVGR
                     MTGRNIWMVDQANLTIDEICATTRHHRMKHPETALVVVDYLGLIKTRSTGRHDLAVGE
                     ISKGLKSLAKSGGFPLIALSQLSRGVESRPNKRPMNSDLKNSGEIEADADIILMLYRD
                     EVYNPETQARGIAEINITKQRNGTLGTIYRRFHNGHFLPVDQESARVLSTPMTPGNPR
                     RYSNNRMSGSKTERLF"
     gene            complement(5454..6332)
                     /locus_tag="ACHOSC_RS16680"
                     /old_locus_tag="pgaptmp_003336"
     CDS             complement(5454..6332)
                     /locus_tag="ACHOSC_RS16680"
                     /old_locus_tag="pgaptmp_003336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706613.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HGU4549224.1"
                     /translation="MMTFNLREQQTRLQARMDELRAEIAFAQKGEKPWPYRSCWMREG
                     RGCCEKHGEYHTHILVWSDRNGEDREKISCCPDCLIAEANDLTMELSSLKAEELTDNA
                     GIALRFRDCEFDNYLEVNPDAARNLAACRRYAENWPDMLENGTSLVMTGSCGTGKNHL
                     AVSMAKHIIRNYLASVEITDVMRLTRAVKNCWRNDSEKTADDVIEHYASLDLLIIDEV
                     GVQFGSAAEMAILQEIINARYEGILPTILISNLSPEELWAFISPRIADRITNGGRNWL
                     SFNWPSYRSRIGGVAA"
     gene            complement(6343..7251)
                     /locus_tag="ACHOSC_RS16685"
                     /old_locus_tag="pgaptmp_003337"
     CDS             complement(6343..7251)
                     /locus_tag="ACHOSC_RS16685"
                     /old_locus_tag="pgaptmp_003337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001247840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HGU4549225.1"
                     /translation="MRPSDLLLDFGHPVAYYPGLVKYMGSPHAVIFFGQIFYWQDKAH
                     AAEGVHKTREEIQHETGLTFEQQAVARKHLVSRGILVETNKRLEHKMFYRIDCERLNE
                     IINENNQFSRNGETRFRETVKPNFAEEGKPSPRTRETTRRGEGKTNFDLTENTTEITS
                     ENTTESKNTIGASADASAPARSARQEYSPEFEQAWQEYPKRAGGNSKSAAFKAWKARI
                     REGVTPETMLNGVKRYAAWVRVSGNTGTQFVKQASTFFGPDRHFEDFWQHPAAPGGGR
                     QRQVDVLAGLGAMSDKFGKSSDKLTF"
     gene            complement(7238..7471)
                     /locus_tag="ACHOSC_RS16690"
                     /old_locus_tag="pgaptmp_003338"
     CDS             complement(7238..7471)
                     /locus_tag="ACHOSC_RS16690"
                     /old_locus_tag="pgaptmp_003338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000621187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4549226.1"
                     /translation="MIRRVVNSLYHRYNRCPRVGQWFTTSNGHVLRVCLVNTESQMVV
                     CQVQGRTHTLSYPLVAFQSGKMFKRLGGGYASV"
     gene            complement(7468..8130)
                     /locus_tag="ACHOSC_RS16695"
                     /old_locus_tag="pgaptmp_003339"
     CDS             complement(7468..8130)
                     /locus_tag="ACHOSC_RS16695"
                     /old_locus_tag="pgaptmp_003339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000587261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ash family protein"
                     /protein_id="HGU4549227.1"
                     /translation="MIISRPNIDSAISGRYTVSAPYKAGAGIGTLELLEAIYDAPASF
                     LSSMLAHARIMVGWAGELKGSPVSLSAGSSNPVQSATSEIGTSGGGSFSLLKEAAIMA
                     TVPTSPYLKIEVVNGKAVIFSLHVACHFKRMHQNIVDKIEYLNCSREFFTRNFIPGTY
                     HIYGDSLRGYYITLDGLMMLQLGLSLRTMRYYESCIEAFHEAEISLSHTAFRRNQWEV
                     RP"
     gene            complement(8238..8945)
                     /locus_tag="ACHOSC_RS16700"
                     /old_locus_tag="pgaptmp_003340"
     CDS             complement(8238..8945)
                     /locus_tag="ACHOSC_RS16700"
                     /old_locus_tag="pgaptmp_003340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001435006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rha family transcriptional regulator"
                     /protein_id="HGU4549228.1"
                     /translation="MNTAIFNDKASMTSVEIAELVGSQHKDVKRSIERLMGKGVIRSA
                     PMANFEIINNLGLKRNVAAYVFEGEQGKRDSIIVVAQICPEFTARLVDRWRELEEQIR
                     KPMSEIEMVAAMALEAVRQQKRITQVEEKVSHVAETVEQIKKGNIREGYAGYRQLKAK
                     TGLSDDKCRNLVNAYQIPTDTHEFMTPDGLLSRRAIVAVEPFMAAFYRVMEEAEPRGT
                     RWYHPRMGLFQVIGWQR"
     gene            complement(9027..9260)
                     /locus_tag="ACHOSC_RS16705"
                     /old_locus_tag="pgaptmp_003341"
     CDS             complement(9027..9260)
                     /locus_tag="ACHOSC_RS16705"
                     /old_locus_tag="pgaptmp_003341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000944728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HGU4549229.1"
                     /translation="MLRKLIPQNAIARYIGVTPQAVNLWFSKNSVPSRFVLRVCELVE
                     WKVTPHGLRPDLYPYPEDGIPDSLRKSNGITRD"
     gene            9417..>10083
                     /locus_tag="ACHOSC_RS16710"
                     /old_locus_tag="pgaptmp_003342"
     CDS             9417..>10083
                     /locus_tag="ACHOSC_RS16710"
                     /old_locus_tag="pgaptmp_003342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000793268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XRE family transcriptional regulator"
                     /protein_id="HGU4549230.1"
                     /translation="MKNVKSTENRIAMMLKTKGWSQAELARKLGVSAQSVQYWTTGKT
                     FPRSDKLAQLSVISGYPQSWFLGEDASSTFSSAEKHHTREDSVVFNVLDVEFSCGDGT
                     HVRGDLIDVVRSIELDPEYARRLVGNRAFKNIEIGNARGDSMAPTISPGDLLFLDKTV
                     TYFDGDGIYAFCFDGECYVKRLQKIGSKIMVLSDNPNYQPWSIEKEGMALLYIQSKVI
                     SSVP"
BASE COUNT         2452 a         2744 c         2357 g         2530 t
ORIGIN      
        1 atctcgcgta acgcggcacg acgaccaatt ttaaaattgg tatccagctg accgataata
       61 gttgcgctaa tcgcattaat ttccgaggta aacgcagaca acgcaggctg attacgtaag
      121 acctgttcga tcttcgcttt ctcggatgcc tcgcattcac catctgcata ggccaccagg
      181 tatgcagcgt taatcaccgc ctgtgccaga tcgcgtttct caaacttttt aatttccgct
      241 gccgctctgc gggttttctt tttgaagatt ccaaacatcg tgacgttcct ttgggtgggt
      301 gagccaacgc ccgggagcga tctgcccaca gagaaagtca cactgaccac tccgtaagct
      361 cacccccgaa aggctctgtg gttgatatgc gccgggcgtg gcgcagatac aaaaaaggcc
      421 cgccgaagcg agcctggaaa ataagtgtgg cgcgttgtag tggagtcgaa ccactgaccg
      481 attgcttaga aggcaattgc tctgtccgac tgagctaaca acgcatgatg ctgataatgg
      541 accgccatcg gggacttgaa ccccgcgcag ccagcttcga aggctggcgc tctgtcccga
      601 tgagctaatg gcggtatgtg atatggtggc ccttgctgga tttgaaccag cgacctggcg
      661 attatgagtc gctcgctctc accactgagc taaagggccg gtagcagaat aataatggtg
      721 cgtaattaat tctgcaatcc catccgtttc aaacgattaa atcctgaact tccctgactg
      781 tctgttcaaa acgtcctgtc tccagctcaa caccaatcgc acaacgcccc agtgccatcg
      841 ccgcttttac cgttgaacct gaacccataa aaaaatctgc aaccaggtct cccggacgac
      901 tgctcgcgtt gattatctgc tgcagcattt ctgccggttt ttcgcacgga tgtttccctg
      961 gatagtactg caccggttta tgcgtccaga catcggtgta cggaacctgc gccgtcacac
     1021 cgaaataccg ccgcaaattt ttatattcac tcagcagttc cgtatactgc cggttcagcc
     1081 cactgtatgt gctgaccagc tggtggtgtg gcttttccag ttccccgcgc tgatgttttt
     1141 ctgccgcaac acgcgcaaac aacgcctgca atttgttgta attaccctcg ttcggtaact
     1201 gccactgact ggtaccaaac cagtgcgaag ccatgttttt ctttccggtg gcttccgcta
     1261 tctgttttga cgttattccc agtgatttac gcgcatcacg aaagtaagaa atcagcgggg
     1321 ccatgacgtg ctgttttagc tcgcgcccct gtgccacata gccatcatct ttcgggcgat
     1381 acggtccctg ataatgttct gcaaacagaa tgcgctctgt tgccggaaaa tacgcccgca
     1441 gactttcctt attgcacccg ttccagcgtc cggacggctt cgcccagata atatggttca
     1501 gcacattaaa gcgctcacgc atcatgattt cggtgtcaga tgccaggcga tgaccaaaga
     1561 acaggtaaag acttccggca ggcttcagta cccgccagaa ctgcgccaga cactggtcca
     1621 gccatttcag gtaatcctca tcacccgtcc actggttatc ccagccctca ggcttcactt
     1681 taaagtacgg cgggtccgtg actatcaggt caacagaatt ttcgggtaac gaccggataa
     1741 attccaggca gtcggcgttg attaactcac aactggatat ttttacagta ttaaacatgg
     1801 atcattaagc ctgtctctga taggctcata ccgcttttgc gcaaagcaga tgggcctgag
     1861 gtttgcttgt gaccccaacg catgagcaga tggctggtga gtgcccctaa cacccaccag
     1921 ccgcccattt accacaaata aaaaagcctt caggactgaa ggcgtctgta acaaccaaac
     1981 tgatagtctg ccagacccgc cataacaagc tgggtcagta ttagctggca gcgttcgcgt
     2041 gaaaggtacg tattctgtgc aatctccccg actgtcgccg gtgcggtggc acttaattca
     2101 ttaaacacca ctctggcggt ttctgtcata tcctgctgtt tcagcatgtc tttttccctt
     2161 ttccggttaa cgtgacacac caataactct tgtcgaaaaa gccagcaagc tgaaagaccg
     2221 gtattcaccg ccaccagcgc gtttaacgta ctggtccgat ttcaggcata aaaaacccgc
     2281 ctgacggcgg atttaagctg tgtggcaaag taaccactct taacatactg acatactttt
     2341 tgcggaccgc actaatcatt ttttactttt ttagcagcca gtcgtccatc tccagtctta
     2401 cccccagcac agacaaacat ccgtcaataa acccttcggc tatctgcatc tcaattcgta
     2461 ttgctttttc gcttttcttt ctcgtcctgg ctatctgtct ttttgatatt cgcaacaaat
     2521 aatgagcaat gagaagcgaa tactcctcag gttttttctg cttcagacgt gcaagacagt
     2581 tttcaatgat aagtccgtca tcatcgcagc aggctggacg tggtttagtg gtagatggta
     2641 aaaggccttt gaatccggca gcgatcggag aatagtccac cccggtgtta ccacttgccg
     2701 cccatgcccc ccagcgttca agaaccatct gaatatcacg catcaacttt ctccacaaaa
     2761 atcaggacag cacaccaatc gccagtgcgc gatcgataaa acgaaatatc agctccagtt
     2821 gggaaccata cttctcttca aatgccacgg tatccgcatg cagttcgtca tggtgttttc
     2881 tgcacaaagg caacacaaaa aggtcatgcg cttttgtacc cattccaccc tgaccatggc
     2941 caatcaggtg atgtggatcg tcggctggct taccacaaca cgcgcaaggc tgtgtcttaa
     3001 cccagcgcgt gtacttttca ttaacccagc gacgacgttt ggggcgtaac ataaaagact
     3061 ccggcgactc cggatccact ttcagcgcca gcaccttttt cgctttatcc tggataatgc
     3121 tggtggcagg aaccgaaggc acaaggtcac tctcccgggt gacagacggc aaaacaggct
     3181 tcggtaatct cagggcctta cgggctgcac tttccggtaa ggcatccgcc aggtcattac
     3241 gaaccagcca ccagcacagt tcaggcattg tcacaacgtg actgtcatca aaaccgagat
     3301 cacgacggac tacggacaac acccagcggg cacagttatc cgttgccatt gactccagac
     3361 gttccgtgaa ctgatcgcga agcaggttat cgcagtgcca gcacagacgg attgcgcccg
     3421 gcgcgtgtcg cattgtggtc atgttctcgc tgtgccatcc ggaatgaggc cactggcagc
     3481 ctttttcacg aagtaaccag ctctcaaggc attccacgcc accagcacga cggatcaccg
     3541 cctcatggcg gaacacggcc cgaacggcag gatcatccgc cagcggttgt gatgctgccg
     3601 gaacggcacc actggcaaaa gatgaataac gttccggctc aggctccagc aggacacgcc
     3661 cctgcataaa caggggcatc agctctgaac cgggtctgaa caagacgatc cccatacgcg
     3721 gggcaatttc aggggtcagc agtgctctca cggtcacctc agcgaacggt attgcatgaa
     3781 cgcaggagaa aaaattcagc catcacgcag taaactcttt caccagcgtt tcaaactggc
     3841 ttacctggcc ttccagttcc gccacgcaat ccaccagctc atccaccgcc ttttgtgtgc
     3901 gatgttttgc ctgcagcaga tcacgaagcg ccggagtaag ctgcttgcgg agcgtatctt
     3961 ttgccacgct cgttttttcc atctgttcag cacaacgaag catctcctgc gcctgccgac
     4021 gaagttgttc cggtgaaaca gtggtggttc tgttgttcaa aataaacgct ccgttttact
     4081 acccgacatg cggttattgc tgtatctgcg cggattgccc ggcgtcatgg gagtggaaag
     4141 aacccgggca ctctcctggt ccacaggcag aaaatgcccg ttatgaaaac gccggtaaat
     4201 ggtacccagc gtgccattac gctgtttcgt gatgttgatt tcagctatgc ctctcgcctg
     4261 tgtctccggg ttgtacacct catccctgta aagcatcaga atgatgtctg catccgcctc
     4321 tatttcccct gagtttttca ggtccgagtt catggggcgt ttattgggtc tggattccac
     4381 gccacgggag agctggctca gagcaatcag cggaaaaccg ccggattttg ccaggctttt
     4441 aagtcccttt gagatttccc ccacagcaag gtcgtgacgc cccgtgctgc gggttttaat
     4501 caggccgagg taatcgacca ccaccagcgc cgtttccggg tgtttcatcc ggtggtgcct
     4561 cgtggttgca catatctcat caatggtcag gtttgcctgg tccaccatcc agatattacg
     4621 ccccgtcatt cgtcccacgc cctgtgagaa acgcgcccag tcttcatctt caaaacgggc
     4681 aacagactta agacgggata ccggcattcc cccggcagca gacaccatac gttcaccaat
     4741 ctgaatgttc gccatctcca tggtgaacag aagcacgcca tgcccctgct cagtcacctt
     4801 gtcgatgata tccagcgcaa gttcggtttt ccccatcgaa ggacgggccg caatgaatac
     4861 caggtcgcct ggctccatac cgcccgtttt tgcgtccagt tcatcaatac cggtcatcag
     4921 cgccctggat ttctccagtc cctgattgcg gcattcaaca cggtcgacca cttccggaag
     4981 gacatcatca atgtgaaccg gctgaatgac gccctttccg gtcgacagtg aggccatcat
     5041 gttctgcgca tccttcaggg catcctcggc tgcttcacag gtatgcgcat cccgtaaatt
     5101 ctgtaatgct tcagtcagtg ttttttctgc atcgcgcagt gcggcattac gccgcaacgc
     5161 tgcgacatag tgctccagtg aagacttcac ccaggttttg cgtccggtgt cggtaatcac
     5221 cggggcaagt tccggcatct cattgcacag cagtacgggg tcaatgacgc cggatacacg
     5281 tgcctgtctg caaattcccg cgtaaatatc ccggtactgt cgtgcagaaa aaacgtccgc
     5341 cgacaatgtg gccagaatat ccatcacttc cggatcagcc ccacgcagaa aaaacgcgcc
     5401 aatgacagcg ccttccaggt catcgttacg ccatgccggg gtgttctggc tggtcatgcg
     5461 gcaacacctc cgatacgaga acggtagctg ggccagttaa acgacaacca gttgcgcccg
     5521 ccattggtga tcctgtcggc aatccgggga ctgatgaacg cccacaattc ttccggtgaa
     5581 aggttgctga tcaagatagt tggcaaaata ccctcatacc gggcattgat aatttcctgc
     5641 aaaatggcca tttcagccgc actgccaaac tgaacgccga cttcgtcgat gatcagcaaa
     5701 tccagtgacg cataatgctc aatgacgtca tccgctgttt tttcactgtc attccgccag
     5761 cagtttttca cagcacgggt aaggcgcatc acgtcggtga tctccacact ggccagatag
     5821 ttacggatga tgtgttttgc cattgatacc gccagatgat ttttcccggt accgcaactg
     5881 ccggtcataa caagactggt accgttctcc agcatatctg gccagttctc cgcatagcgg
     5941 cgacaggccg caagatttct ggctgcgtca ggattaacct ccagataatt atcaaactcg
     6001 cagtcccgaa aacgcagagc aattccggcg ttatcagtca gttcttccgc cttgagggac
     6061 gacagctcca tggtcaaatc gttggcctca gctatcaagc agtcagggca gcatgaaatt
     6121 ttttctctgt cctcgccatt acgatcgctc cacaccagaa tatgtgtgtg gtattcgcca
     6181 tgtttttcgc aacacccgcg cccttcacgc atccagcagg aacgataagg ccatggtttt
     6241 tcgcccttct gagcaaatgc aatctctgcc cgtaactcat ccatccgcgc ctgtagtctt
     6301 gtttgttgtt cacgcaggtt aaacgtcatc atcgctgtca cctcagaatg tcaatttgtc
     6361 actggattta ccgaatttgt cagacatggc tcccaggcca gccaggacat cgacctgtcg
     6421 ctgtcgccca cctccgggag ctgctggatg ttgccagaaa tcttcgaagt gacgatcggg
     6481 tccaaagaac gtcgacgcct gcttcacgaa ctgggtgccg gtatttcctg agacacgcac
     6541 ccaggcagca tagcgtttca caccgttgag catggtttct ggtgtcacac cttccctgat
     6601 tcgggctttc caggctttga aggctgctga cttggaatta ccaccagcac gtttgggata
     6661 ttcctgccag gcctgttcaa attccggtga atattcctgt cgggcagaac gcgctggtgc
     6721 agacgcgtca gcggatgcgc caatagtgtt tttactctct gtagtattct ctgaagtaat
     6781 ctctgttgta ttctctgtaa gatcgaaatt ggttttccct tctccgcggc gagtggtttc
     6841 ccgtgtccgc ggtgaaggct ttccctcctc cgcgaaattg ggttttacag tttcccgaaa
     6901 acgggtttcc ccatttcggg aaaactgatt gttttcattg ataatttcat taaggcgctc
     6961 acaatctata cggtagaaca ttttgtgctc aagacgcttg ttggtttcaa ccaaaatgcc
     7021 tctggacaca agatgcttac gcgctacagc ctgttgttca aatgtaagtc cagtttcgtg
     7081 ttgtatctct tcacgcgttt tatgtacgcc ttccgctgca tgtgctttat cctgccagta
     7141 aaaaatctga ccaaagaaaa taacagcgtg cggacttccc atgtatttaa cgagcccagg
     7201 gtaataagca accggatgcc caaaatcgag cagaagatca gacggacgca tagccacctc
     7261 ccagacgctt aaacattttc ccggactgaa aagccaccag cggataactc agggtatgag
     7321 tacgtccctg aacctggcag acaaccatct ggctttctgt attgaccagg caaacccgca
     7381 gaacgtggcc gttgctggtg gtgaaccact gccccacacg ggggcaacgg ttgtatcggt
     7441 gatacaggga attaacgacg cggcgaatca tgggcgcacc tcccattgat tacggcggaa
     7501 agcggtgtga ctgaggctga tttctgcctc atggaatgct tcaatgcagc tctcgtagta
     7561 ccgcattgtg cgtagactta acccaagctg aagcatcatc agaccatcaa gggtgatgta
     7621 ataaccacgc agggagtcac cgtagatgtg ataagtaccc ggtatgaaat tgcgggtaaa
     7681 aaactcgcgt gagcagttca gatactcgat tttgtcgacg atgttctggt gcatgcgctt
     7741 aaagtggcag gcaacatgca gggagaaaat aacggccttg ccgttgacaa cttcaatttt
     7801 caggtatggg gaagttggga ctgtagccat gatggcagcc tccttgagca gtgaaaaact
     7861 tccaccaccg gaggttccaa tctcactggt ggcggactgg acagggttgg aactaccggc
     7921 gctcaaggaa accggcgagc ctttcagctc ccctgcccag cccaccataa ttctggcgtg
     7981 cgcgagcatg gacgataaaa aagacgctgg cgcgtcgtat atcgcctcga gcaattccag
     8041 ggttccaatc ccggcacccg ctttataagg tgcagagaca gtgtaacgtc ccgaaattgc
     8101 agaatcaata tttggtcttg aaatgatcat atagctgctg atatctttag aactgttctt
     8161 ggatgtttcg gagccgtttt atgcgaaaca gctccccgtt attgatgttg agtgagccgg
     8221 gttactcccg gcttttttca ccgctgccaa ccaataacct gaaataaccc cattctcggg
     8281 tgataccagc gagtccctcg cggttctgct tcctccataa cccgataaaa agcagccata
     8341 aacggttcca cagcaacaat tgcgcgacgt gacaacaatc cgtccggcgt catgaactca
     8401 tgggtgtctg taggaatttg ataggcgttc accagattgc ggcatttatc atctgacaaa
     8461 ccggtttttg ctttcagttg gcgatatccg gcatagccct cacgaatgtt gccctttttg
     8521 atttgctcaa ctgtttcggc aacgtggctg actttttctt ccacctgagt gatccgtttc
     8581 tgctgacgaa cagcttcaag agccatcgcg gcaaccattt cgatttcgct cattggctta
     8641 cggatctgtt cttccagttc acgccagcgg tctaccaggc gggcggtgaa ttcagggcaa
     8701 atctgcgcga caacaatgat actgtcgcgt ttgccttgtt caccttcgaa tacgtaagca
     8761 gctacatttc ttttcaatcc taagttgttg attatttcga aattcgccat tggcgcactt
     8821 cggataactc ccttccccat gagtctttca atgctgcgtt taacatcttt gtgttggctg
     8881 cccaccagct ctgcaatctc aacgctggtc atggatgctt tatcgttaaa aattgcggtg
     8941 ttcatctgat tgctccttga ggatgacatt tcagaactca cgccaaaacg tattcatcgt
     9001 gaattactgc ttgtgacagt cagtctttaa tctctggtaa taccgtttga ttttcgtaac
     9061 gaatcaggaa ttccatcttc gggataaggg tatagatcag gtcttaaccc atgcggagta
     9121 accttccatt caaccaattc acacacccgt aaaacaaaac gagaaggaac ggagttttta
     9181 gaaaaccaca gattcaccgc ttgtggcgta acaccgatgt atctcgctat ggcgttttga
     9241 gggatcaatt tacgcaacat gtcgtaatca ttcagtttta tcacagcgca gctccaacat
     9301 taactttaca aatcaagaat acatcaagaa taaattaaca tgcaagtttc aaaaggatcg
     9361 aatacactaa aatcaagtta atatttatgt gtataaagct tcgacaggaa ctcaccatga
     9421 agaatgtaaa aagcacagaa aatcggatag caatgatgct gaaaacgaaa ggctggagtc
     9481 aggctgaact agcccgtaag ctgggtgtaa gcgcgcaatc agtacaatac tggactacag
     9541 gaaagacgtt tcccagaagc gataaacttg cacagctatc agtaattagt ggttatccac
     9601 aatcctggtt tttgggtgaa gatgcctcat cgacattctc ttcagctgaa aaacaccata
     9661 caagagaaga cagcgttgtg ttcaatgtac tggatgttga attcagttgc ggcgacggaa
     9721 ctcatgtacg gggagatcta attgatgtag tacgctcaat agaacttgat cctgaatatg
     9781 cccgccgcct ggtcggtaac agagcattca agaacataga aatcggtaac gccagagggg
     9841 acagcatggc cccaacaatc tccccgggag acttactgtt tttagataaa acagtaactt
     9901 actttgacgg cgatggcatt tatgcatttt gctttgatgg cgaatgttac gtcaaacgac
     9961 ttcaaaaaat tggaagcaag atcatggtct tatcagacaa tcccaactat cagccatgga
    10021 gcatcgaaaa agagggaatg gcgctgctct atatccagtc aaaagtaatc tcatctgtac
    10081 cat
//
LOCUS       DAZKHF020000168        10027 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2201S31241
            SAMN43267800-rid25106413.denovo.169, whole genome shotgun sequence.
ACCESSION   DAZKHF020000168 DAZKHF020000000
VERSION     DAZKHF020000168.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267800
            Sequence Read Archive: SRR30307263
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 10027)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10027)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 10027)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350581.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350581.2
            Genome Coverage       :: 151x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:21:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,652
            CDSs (total)                      :: 5,557
            Genes (coding)                    :: 5,320
            CDSs (with protein)               :: 5,320
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 237
            CDSs (without protein)            :: 237
            Pseudo Genes (ambiguous residues) :: 0 of 237
            Pseudo Genes (frameshifted)       :: 102 of 237
            Pseudo Genes (incomplete)         :: 130 of 237
            Pseudo Genes (internal stop)      :: 52 of 237
            Pseudo Genes (multiple problems)  :: 41 of 237
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10027
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267800-rid25106413.denovo.169"
                     /serotype="O 26:H11"
                     /isolate="2201S31241"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-01"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            complement(1..149)
                     /locus_tag="ACHOSC_RS16715"
                     /old_locus_tag="pgaptmp_003343"
                     /pseudo
     CDS             complement(1..149)
                     /locus_tag="ACHOSC_RS16715"
                     /old_locus_tag="pgaptmp_003343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612086.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
     misc_feature    complement(<1..84)
                     /locus_tag="ACHOSC_RS16715"
                     /old_locus_tag="pgaptmp_003343"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01497"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="AL1L pseudoknot; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /pseudo
                     /db_xref="RFAM:RF01497"
     gene            complement(535..1728)
                     /locus_tag="ACHOSC_RS16720"
                     /old_locus_tag="pgaptmp_003344"
     CDS             complement(535..1728)
                     /locus_tag="ACHOSC_RS16720"
                     /old_locus_tag="pgaptmp_003344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HGU4549231.1"
                     /translation="MSPGIEDTPQKPLSCWPLAFSAGLLGIGQNGLLVVLPVLVIQTN
                     LSLSVWAALLMLGSMLFLPSSPWWGKQISRTGSKPVVLWALGGYGISFTLLGLGSVLM
                     ATSAITTAVGLGILIIARIAYGLTVSAMVPACQVWALQRAGEGNRMAALATISSGLSC
                     GRLFGPLCAAAMLAIHPLAPLGLLMAAPVLALLMLLRLPGTPPQPTPERKSVSLKRDC
                     LPYLLCAILLAAAVSMMQLGLSPALTRQFATDTTAISQQVAWLLGLSAVAALIAQFGV
                     LRPQRLTPVALLLSAGVLMSGGLAIMLSEQLWLFYPGCAVLSFGAALATPAYQLLLND
                     KLADGAGAGWLATSHTLGYGLCALLVPLVSKTGVAIALIMAALFATILFTIVSVFIWH
                     YRAIK"
     gene            1864..3588
                     /gene="iucA"
                     /locus_tag="ACHOSC_RS16725"
                     /old_locus_tag="pgaptmp_003345"
     CDS             1864..3588
                     /gene="iucA"
                     /locus_tag="ACHOSC_RS16725"
                     /old_locus_tag="pgaptmp_003345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001314127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aerobactin synthase IucA"
                     /protein_id="HGU4549232.1"
                     /translation="MTLPSEKPATDVAAQCFLNALIRETTDWKLTEYPPDELIIPLDE
                     QKSLHFRVAYFSPTQHHRFAFPARLVTASGSYPVDFTTLSRLIIDKLRHQLFLPVPLC
                     ETFHQRVLESHAHTQQAIDARHDWTALREKALNFGEAEQALLTGHAFHPAPKSHEPFN
                     RREAERYLPDMAPHFPLRWFSVDKTQIAGESLHLNLQQRLTRFAAENAPQLLNELSDN
                     QWLFPLHPWQGEYLLQQGWCQALVAKGLIKDLGEAGTSWLPTTSSRSLYCATSRDMIK
                     FSLSVRLTNSIRTLSVKEVKRGMRLARLAQTDGWQMLQVRFPTFRVMQEDGWAGLLDL
                     NGNIMQESLFALRENLLVDQPKSQTNVLVSLTQAAPDGGDSLLVSAVKRLSDRLGITV
                     QQAAHAWVDAYCQQVLKPLFTAEADYGLVLLAHQQNILVQMLGDLPVGFIYRDCQGSA
                     FMPHATDWLDSIGEAQAENIFTHEQLLRYFPYYLLVNSTFAVTAALGAAGLDSESNLM
                     ARVRASLAEVRDQVTHKTCLNYVLESPYWNVKGNFFCYLNDHNENTIVDPSVIYFDFA
                     NPLQAQEV"
     gene            3589..4536
                     /gene="iucB"
                     /locus_tag="ACHOSC_RS16730"
                     /old_locus_tag="pgaptmp_003346"
     CDS             3589..4536
                     /gene="iucB"
                     /locus_tag="ACHOSC_RS16730"
                     /old_locus_tag="pgaptmp_003346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N(6)-hydroxylysine O-acetyltransferase IucB"
                     /protein_id="HGU4549233.1"
                     /translation="MSEANIIHSRYGLRCEKLDKPLNLGWGLDNSAVLHCPGELPTGW
                     LCDALDQIFIAAPQLSAVALPWAEWCEEPQALTLFGQVKSDIIHRTAFWQLPLWLSSP
                     ANRASGEMVFDAEREIYFPQRPPRPQGEVYRRYDPRIRRMLSFRIADPVSDAERFTRW
                     MNDPRVEYFWEQSGSLEVQTAYLERQLTGKHAFPLIGCFDDRPFSYFEIYWAAEDRIG
                     RHYSWQPFDRGLHLLVGEQQWRGAHYVQSWLRGLTHYLLLDEPRTQRTVLEPRTDNQR
                     LFRHLEPAGYRTIKEFDFPHKRSRMVMADRHHFFTEVGL"
     gene            4536..6278
                     /gene="iucC"
                     /locus_tag="ACHOSC_RS16735"
                     /old_locus_tag="pgaptmp_003347"
     CDS             4536..6278
                     /gene="iucC"
                     /locus_tag="ACHOSC_RS16735"
                     /old_locus_tag="pgaptmp_003347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NIS family aerobactin synthetase IucC"
                     /protein_id="HGU4549234.1"
                     /translation="MNHKDWDFVNRRLVAKMLSEMEYEQVFHAESQGDDHYCINLPGA
                     QWRFIAERGIWGWLWIDAQTLRCTDEPVLAQTLLMQLKPVLSMSDATVAEHMQDLYAT
                     LLGDLQLLKARRGLSASDLINLDADRLQCLLSGHPKFVFNKGRRGWGKEALERYAPEY
                     ANTFRLHWLAVKREHMIWRCDNDLDIQQLLTAAMDPQEFTRFSQVWQENGLDHNWLPL
                     PVHPWQWQQKIATDFIADFAEGRMVSLGEFGDQWLAQQSLRTLTNASRRGGLDIKLPL
                     TIYNTSCYRGIPGRYIAAGPLASRWLQQVFATDATLVQSGAVILGEPAAGYVSHEGYA
                     ALARAPYRYQEMLGVIWRENPCRWLKPDESPVLMATLMECDENNQPLAGAYIDRSGLD
                     AETWLTQLFRVVVVPLYHLLCRYGVALIAHGQNITLAMKEGVPQRVLLKDFQGDMRLV
                     KEAFPEMDSLPQEVRDVTSRLSADYLIHDLQTGHFVTVLRFISPLMVRLGVPERRFYQ
                     LLAAVLSDYMNKHPQMSERFALFSLFRPQIIRVVLNPVKLTWPDQDGGSRMLPNYLEN
                     LQNPLWLVTQEYES"
     gene            6275..7612
                     /gene="iucD"
                     /locus_tag="ACHOSC_RS16740"
                     /old_locus_tag="pgaptmp_003348"
     CDS             6275..7612
                     /gene="iucD"
                     /locus_tag="ACHOSC_RS16740"
                     /old_locus_tag="pgaptmp_003348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent L-lysine N(6)-monooxygenase
                     IucD"
                     /protein_id="HGU4549235.1"
                     /translation="MKKSVDFIGVGTGPFNLSIAALSHQIEELNCLFFDEHPHFSWHP
                     GMLVPDCHMQTVFLKDLVSAVAPTNPYSFVNYLVKHKKFYRFLTSRLRTVSREEFSDY
                     LRWAAEDMNNLYFSHTVENIDFDKKSRLFLVQTSRGEYFARNICLGTGKQPYLPPCVK
                     HVTQSCFHASEMNLRRPDLSGKRITVVGGGQSGADLFLNALRGEWGEAAEINWVSRRN
                     NFNALDEAAFADEYFTPEYISGFSGLKEDIRHQLLDEQKMTSDGITADSLLTIYRELY
                     HRFEVLRKPRNIRLLPSRSVTTLESSGPGWKLLMEHHLDRGRESLESDVVIFATGYRS
                     ALPQILPSLMPLITMHDKNTFKVRDDFTLEWSGPKENNIFAVNASMQTHGIAEPQLSL
                     MAWRSARILNRVLGRDLFDLSMPPALIQWRSGSRKKPQPEAAALTHYTTNIQE"
     gene            7618..9813
                     /gene="iutA"
                     /locus_tag="ACHOSC_RS16745"
                     /old_locus_tag="pgaptmp_003349"
     CDS             7618..9813
                     /gene="iutA"
                     /locus_tag="ACHOSC_RS16745"
                     /old_locus_tag="pgaptmp_003349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709458.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferric aerobactin receptor IutA"
                     /protein_id="HGU4549236.1"
                     /translation="MRKKYMPRALGPLLLVVLSPAVAQQNDDNEIIVSASRSNRTVAE
                     MAQTTWVIENAELEQQIQGGKELKDALAQLIPGLDVSSQSRTNYGMNMRGRPLVVLID
                     GVRLNSSRSDSRQLDSVDPFNIDHIEVISGATALYGGGSTGGLINIVTKKGQPETMME
                     FEAGTKSGFNSSKDHDERIAGAVSGGNDHISGRLSVAYQKFGGWFDGNGDATLLDNTQ
                     TGLQHSNRLDIMGTGTLNIDESRQLQLITQYYKSQGDDNYGLNLGKGFSAISGSSTPY
                     VSKGLNSDRIPGTERHLISLQYSDSDFLRQELVGQVYYRDESLRFYPFPTVNANKQAT
                     AFSSSQQDTDQYGMKLTLNSQLMDGWQITWGLDAEHERFTSNQMFFDLAQASASGGLN
                     NHKIYTTGRYPSYDITNLAAFLQSSYDINDIFTVSGGVRYQYTENRVDDFIDYTQQQK
                     IAAGKAISADAIPGGSVDYDNFLFNAGLLMHITERQQAWFNFSQGVALPDPGKYYGRG
                     IYGAAVNGHLPLTKSVNVSDSKLEGVKVDSYELGWRFTGDNLRTQIAAYYSLSNKSVE
                     RNKDLTISVKDDRRRIYGVEGAVDYLIPDTDWSTGVNFNVLKTESKVNGQWQKYDVKE
                     SSPSKATAYISWAPEPWSLRVQSTTSFDVSDAEGNDINGYTTVDFISSWLLPVGTLSF
                     SVENLFDRDYTTVWGQRAPLYYSPGYGPASLYDYKGRGRTFGLNYSVLF"
BASE COUNT         2369 a         2620 c         2659 g         2379 t
ORIGIN      
        1 gaccccgtac tgctcacgca gcttatccag cagtggcatc attttttcca gaggcggtcg
       61 aactccgcct tcgcaaaata agcggaagcc tggcgaagga tatcgttact gcggcgcagt
      121 tcacgatttt cacgctccag ctctttcagg tgtcaacgcc actctgagag agttttatgc
      181 atcccgtatt gggcactatg atgccggaaa tctgcgaggc tcgacacctc tctgattttc
      241 ccctttattt atcacggtgc cccagatcta atgttgggga tgcgttcatc tcattcccaa
      301 tgagtaatcg cactgttctc attgtctgca ggcatccgga cagagccatg tgatgttgca
      361 ctcacaacca gcatgacttc aatgcaactt aagtgacacc ggccggtttt ataccgaaca
      421 taccctgtac catgcggtgc atttttgcca gtgttactgc ctgacatgct ttcatcagac
      481 gcaggttctc tctcatatcc tctgagttac tccggtaagg tgatgatcat atctttattt
      541 gatagcacgg taatgccaga taaatacaga cacaatggta aataatatag ttgcaaataa
      601 tgccgccata atcagcgcga ttgcgacacc tgttttcgat accagcggca ccagcaaagc
      661 gcacagtcca tagccaagtg tgtgactggt agcgagccag cccgcgcctg cgccatcagc
      721 cagcttatca ttcagtagaa gttgataagc gggtgttgcc agagctgctc caaatgacag
      781 cactgcacag cccgggtaaa acaaccatag ctgttcggag agcatgatag ccagaccacc
      841 actcatcaac actccggcac tcaggagcag ggccaccgga gtcaggcgct gcggacggag
      901 taccccgaac tgcgcgataa gcgcagctac tgcggacaga cccaacagcc aggccacctg
      961 ttggctaatg gcggtggtat ccgtggcgaa ctggcgagta agggcaggcg aaagtccaag
     1021 ctgcatcatg ctcaccgccg cagccagtaa tattgcgcaa agcagataag gcagacaatc
     1081 ccgcttcaga ctgacgctct tgcgctccgg tgtgggctgt ggtggtgtgc cgggcaaccg
     1141 cagcagcatc agcagcgcca gcactggggc tgccatcagc agccccagtg gcgccagagg
     1201 atgaatggcc agcattgctg ccgcgcacag cggcccgaat agcctgccgc aactcaggcc
     1261 ggagctgatg gttgccagag cggccatgcg attcccttct cctgctctct gcaatgccca
     1321 gacctgacag gctggcacca ttgccgacac ggtcagcccg taggcgatcc gggcgatgat
     1381 taatattccc agtcccaccg ctgttgttat ggcgctggta gccatcagca cgcttcccag
     1441 ccctagcagg gtaaagctta tgccatatcc tcccagagcc cacaacacca caggtttact
     1501 gccagtacgg gaaatttgct ttccccacca cggggacgat ggcagaaaca gcattgagcc
     1561 aagcatcagc agggcagccc atacagacag actcagattt gtctgtatga ccagaacagg
     1621 gagcacaacc agcagaccgt tctgcccgat accgagaagc ccggcactga acgcaagtgg
     1681 ccagcaggac agtggttttt ggggcgtatc ttcgatccca ggtgacattt taatgtttca
     1741 actccatgta ttaattgtgt ttatttgtaa aattaattta tctgacaata acatttctta
     1801 ttgataatga gaatcattat tgacataatt gttattattt tactgtgtgg gagctgtttg
     1861 actatgaccc tgccctctga aaaaccagcc acagatgtgg ctgcgcagtg cttcctgaat
     1921 gcactgattc gtgaaactac agactggaaa ctgacagaat acccgccaga cgaattgatc
     1981 atcccgctgg atgagcagaa atcgctccat ttcagagtgg cttatttctc cccaacccag
     2041 catcaccgct ttgcatttcc tgcacgtctg gtcacggcat caggcagtta tcctgtcgac
     2101 tttaccactc tctcccggct tattattgat aagctacggc atcaactgtt tctgcccgtt
     2161 cccctctgcg aaactttcca ccagcgcgtg ctggaaagcc acgcccatac gcaacaggca
     2221 attgatgccc gtcatgactg gaccgccctg cgtgaaaaag cgttgaattt tggcgaagct
     2281 gagcaggcgc tgctgacagg acacgctttc caccctgcgc ctaagtctca tgaaccgttt
     2341 aaccggcggg aggctgaacg ctacctgcct gatatggcac cccacttccc actgcggtgg
     2401 ttttcggtgg ataaaacgca aatcgccggt gaaagtttgc atctcaacct tcaacagcgg
     2461 ctgacgcgat ttgccgcaga gaatgcgcct cagttactca acgaattaag tgacaaccaa
     2521 tggctgttcc cgttgcaccc gtggcagggg gaatatcttt tgcagcaggg gtggtgccag
     2581 gcacttgttg ctaaggggct gattaaagac ttaggtgagg ccggcacgtc gtggctgccg
     2641 accacctctt cccgttccct ctactgtgcc accagccgcg atatgatcaa gttctccctg
     2701 agcgtacggc tgaccaactc catccgtact ctgtccgtga aagaagtgaa gcgtgggatg
     2761 cgcctggcac gcctggctca aaccgacggc tggcagatgc tacaggtccg cttcccgact
     2821 ttccgggtaa tgcaggagga tggctgggcc gggctgctcg atcttaacgg caacatcatg
     2881 caggaaagtc tgtttgccct gcgtgaaaat ctgctggtgg atcagccgaa aagccagacc
     2941 aacgtactgg tctccctgac tcaggccgca cctgatggcg gtgattcgct gctggtttcg
     3001 gcggtaaaac gcctgagcga tcgcctcggt atcactgtgc aacaggccgc ccatgcatgg
     3061 gtcgatgcat actgtcagca agtgctaaag ccgctgttta cggctgaagc ggattacggc
     3121 ctggtgctgc tggcgcatca gcaaaatatt cttgtccaga tgcttgggga tctgccggtc
     3181 ggatttattt accgtgactg tcagggcagc gcttttatgc ctcacgcgac agactggctc
     3241 gattctattg gcgaggcgca ggcggaaaat attttcaccc atgagcagtt gctgcgctat
     3301 ttcccttatt acctgctggt taactccact tttgctgtga ccgctgcgct gggggctgcc
     3361 gggctggaca gcgaatcgaa tctgatggct cgtgtacgag catcgctggc tgaagtgcgt
     3421 gatcaggtga ctcataaaac ctgtctcaac tacgtgctgg aaagtccgta ctggaacgta
     3481 aaaggtaact ttttctgtta tctgaacgat cataacgaga acaccattgt tgacccttcg
     3541 gtgatctatt tcgatttcgc taacccgctg caggctcagg aggtctgaat gtctgaggca
     3601 aacattattc acagcagata tggactgcgc tgtgaaaaac tcgacaagcc cctgaatctt
     3661 ggctggggac tggacaatag cgcggtgttg cactgtcccg gggagctgcc gacagggtgg
     3721 ctgtgcgatg cgctggatca gatatttatc gccgcaccac aactgtcagc agtggctctc
     3781 ccctgggccg aatggtgtga ggagccacag gcgctgacgc tttttgggca ggtaaaaagc
     3841 gacattatcc atcgtaccgc cttctggcag ttaccgttat ggttgagttc accggcaaac
     3901 cgggcctccg gtgaaatggt ttttgatgca gagcgtgaga tttatttccc gcagcgccca
     3961 ccccgtccgc agggtgaggt ttaccgccgt tacgatccac gtattcgcag gatgctgagt
     4021 ttccgcattg ccgatcccgt ttctgatgca gaacgtttca cccgctggat gaatgatccg
     4081 cgcgttgagt atttctggga gcaaagtggc tcactggagg tacagaccgc ctatctggag
     4141 cgccagctca ccggtaaaca tgcattcccg ctgattgggt gcttcgacga tcggccattc
     4201 agctattttg aaatctactg ggcggcggaa gaccgcattg gtcgtcacta ttcgtggcag
     4261 ccctttgacc gtggcctgca tctgctggtt ggtgaacagc aatggcgcgg ggcccactat
     4321 gtgcaaagct ggctgcgcgg gctgacacat tacctgctgc tggatgagcc ccgtacgcag
     4381 cgcacagtac tggagccacg caccgataac cagcgcctgt tccgccatct tgagcctgcg
     4441 ggataccgga caattaaaga gttcgacttc ccacataagc gctcgcgcat ggtgatggcg
     4501 gatcgtcatc acttcttcac ggaggtcggt ctgtaatgaa tcacaaggac tgggattttg
     4561 ttaaccgtcg gctggtggca aaaatgttgt ctgagatgga gtatgagcag gttttccacg
     4621 cggaatctca gggcgatgac cactactgca ttaacctgcc gggagcacaa tggcgcttta
     4681 tcgctgaacg tggtatctgg ggctggctgt ggattgatgc ccaaacgttg cgctgcacgg
     4741 acgaaccagt actggcgcag acgctgctga tgcagctaaa gccggtgctg tcaatgagtg
     4801 atgccactgt tgcagagcat atgcaggatt tgtatgccac gctactgggc gacctgcaac
     4861 tgctgaaggc ccgtcgcgga ctgagtgcca gtgacctgat taatcttgat gccgatcgtc
     4921 tgcaatgcct gctgagcggt catcctaaat tcgtttttaa taaaggtcgc cgtggctggg
     4981 gcaaagaggc gctggaacga tatgcgccag agtatgccaa caccttcaga ctgcactggc
     5041 tggcggtaaa acgtgaacat atgatctggc gctgcgacaa cgatctggat attcagcagt
     5101 tgttgactgc cgcaatggat ccgcaggagt ttacacgctt cagtcaggtc tggcaggaaa
     5161 acgggctgga tcacaactgg ctgccgctgc cggtacatcc gtggcagtgg cagcaaaaaa
     5221 tcgccaccga cttcatcgct gattttgccg aaggcaggat ggtgtctctc ggcgagtttg
     5281 gcgaccagtg gctcgcccag cagtcgctgc gtaccctgac caacgccagc cgccggggag
     5341 ggctggatat caagctgccg ctgaccatct acaacacctc atgctaccgg gggatccctg
     5401 gcagatatat cgctgccgga ccactggctt cacgctggct gcaacaggtt tttgcgaccg
     5461 acgccaccct ggtgcaaagc ggcgcagtga tccttggtga accggctgca ggctatgtgt
     5521 cccatgaagg ctatgccgcg cttgcccggg ctccctatcg ctaccaggaa atgcttggtg
     5581 ttatctggcg ggagaatccg tgccgctggc tgaaaccgga tgaaagtccg gttctgatgg
     5641 caacactgat ggagtgcgac gaaaacaatc agccgctggc aggcgcatat atcgaccgct
     5701 ccggactgga cgctgaaacc tggcttacgc aattgttccg ggtggttgtg gttccgctgt
     5761 atcacctgct ttgccgctac ggtgtcgcgc ttattgcaca cggacaaaat ataacgctcg
     5821 ccatgaaaga gggggtacca cagcgtgttc tgctgaaaga cttccagggc gatatgcggc
     5881 tggtgaaaga agcgtttccc gaaatggact ctttgcctca ggaggtgcgt gatgtcacat
     5941 cccgtctgag tgcggactac ttaatccatg atttgcagac gggtcacttc gtgacagtac
     6001 tgcgttttat ttcgccactg atggttcgtc ttggcgtacc tgaaaggcga ttttatcaac
     6061 tgctggcagc agtgttgagt gattacatga acaaacatcc acaaatgtca gagcgttttg
     6121 cgcttttctc actcttcagg ccacaaatca ttcgcgtggt gctgaacccg gtaaaactga
     6181 cctggccgga ccaggatggc ggcagtcgca tgctgccgaa ttaccttgag aatctgcaaa
     6241 atccgctgtg gctggtaact caggaatatg aatcatgaaa aaaagtgtcg attttattgg
     6301 tgtcgggaca gggccattta atctcagtat tgctgcattg tcacatcaga tcgaagaact
     6361 gaactgtctc ttctttgacg aacatcctca tttttcctgg catccgggta tgctggtacc
     6421 ggattgtcat atgcagactg tctttctgaa agatctggtc agtgcagttg cacctacaaa
     6481 tccctacagc tttgttaact atctggtgaa gcacaaaaag ttctatcgct tccttacaag
     6541 cagactacgt acagtatccc gtgaagagtt ttctgactat ctccgctggg ctgctgaaga
     6601 tatgaataac ctgtatttca gtcataccgt tgaaaatatt gatttcgaca aaaaaagtcg
     6661 attgtttctg gttcagacca gtcggggaga atattttgcc cgcaatatct gccttggtac
     6721 aggaaaacaa ccttatttac caccctgtgt gaagcatgtg acacaatcct gtttccatgc
     6781 cagtgaaatg aatcttcgtc ggccggacct gagtggaaaa cggataactg tggttggtgg
     6841 aggacagagt ggtgcagacc tgttccttaa tgcattacgc ggggaatggg gagaagcggc
     6901 ggaaataaac tgggtctcac ggcgtaataa ttttaacgca ctggatgagg ctgcttttgc
     6961 tgatgagtat tttacacctg aatatatttc aggcttctcc ggactgaagg aagatattcg
     7021 ccatcagtta ctggatgagc agaaaatgac atcggatggc atcactgccg attctttact
     7081 gaccatttat cgtgagttgt accaccgttt tgaagttctg agaaaaccaa gaaatatccg
     7141 tctgctaccc agccgctcgg taacaactct ggaaagtagt ggtccgggct ggaagttatt
     7201 gatggaacat catctggatc ggggcagaga gagcctggaa agtgatgtgg tgattttcgc
     7261 cacaggttac cgttctgcat tgccacaaat acttccctca ctgatgccct tgatcaccat
     7321 gcacgataag aacaccttta aagtgcgtga tgacttcact ctggaatgga gtggcccgaa
     7381 agaaaataac atctttgcgg tcaacgccag catgcaaact catggcatcg ccgaacccca
     7441 gctcagcctg atggcctgga gatctgcacg tattcttaat cgcgtactgg ggcgtgattt
     7501 attcgatctc agtatgccgc ccgcactgat tcagtggcgc agcggcagcc ggaaaaaacc
     7561 gcagccggag gctgctgctt taactcacta tacaacaaat attcaggaat aacgatgatg
     7621 cgcaaaaagt atatgccccg ggctcttggt ccgctgcttc ttgtcgtgct gtcaccagct
     7681 gtcgcccagc aaaacgatga taatgagatc atagtgtctg ccagccgcag caatcgaact
     7741 gtagcggaga tggcgcaaac cacctgggtt atcgaaaatg ccgaactgga gcagcagatt
     7801 cagggcggta aagagctgaa agacgcactg gctcagttaa tccccggcct tgatgtcagc
     7861 agccagagcc gaaccaacta cggtatgaac atgcgtggcc gcccgctggt tgtcctgatt
     7921 gacggtgtgc gcctcaactc ttcacgttcc gacagccgac aactggactc tgtcgatcct
     7981 tttaatatcg accatattga agtgatctcc ggcgcgacgg ccctgtacgg tggcgggagt
     8041 accggagggt tgatcaacat cgtgaccaaa aaaggccagc cggaaaccat gatggagttt
     8101 gaggctggca caaaaagtgg ctttaacagc agtaaagatc acgatgagcg cattgccggt
     8161 gctgtctccg gcggaaatga ccatatctcc ggacgtcttt ccgtggcata tcagaaattt
     8221 ggcggctggt ttgacggtaa cggcgatgcc accctgcttg ataacaccca gaccggcctg
     8281 cagcactcca atcggctgga catcatggga accggtacgc tgaacatcga tgaatcccgg
     8341 cagcttcaac tgataacgca gtactataaa agtcaggggg acgacaatta cgggcttaat
     8401 ctcgggaaag gcttttccgc catcagcggg agcagcacac catacgtcag taaggggctg
     8461 aattctgacc gcattcccgg cactgagcgg catttgatca gcctgcagta ctctgacagt
     8521 gatttcctga gacaggaact ggtcggtcag gtttactacc gcgatgagtc gttgcggttc
     8581 tacccgttcc cgacggtaaa tgcgaataaa caggcgacgg ctttctcctc gtcacagcag
     8641 gataccgacc agtacggcat gaaactgact ctgaacagcc aacttatgga cggctggcaa
     8701 atcacctggg ggctggatgc tgagcatgag cgctttacct ccaaccagat gttcttcgat
     8761 ctggctcagg caagtgcttc cggagggctg aacaaccata agatttacac caccgggcgc
     8821 tatccgtcat atgacatcac caatctggcg gccttcctgc aatccagcta tgacattaat
     8881 gatattttta ccgtcagcgg tggcgtacgc tatcagtata ctgagaacag ggtagatgat
     8941 tttatcgact acacgcagca acagaagatt gctgccggga aggcgatatc tgccgacgcc
     9001 attcctggtg gttcggtaga ttacgataac tttctgttca atgctggtct gctgatgcac
     9061 atcaccgaac gtcagcaggc atggttcaat ttttcccagg gggtggcatt gccggatccg
     9121 gggaaatatt atggtcgcgg catctatggt gcagcagtga acggccatct tcccctgaca
     9181 aagagcgtga acgtcagcga cagtaagctg gaaggcgtga aagtcgattc ttatgaactg
     9241 ggctggcgct ttaccggtga caacctgcgg actcaaatcg cagcatatta ctcgctttcc
     9301 aataagagcg tggaaaggaa taaagatctg accatcagtg tgaaggacga caggcgccgt
     9361 atttacggcg tggaaggtgc ggtggactac ctgatcccgg atactgactg gagtaccggt
     9421 gtgaacttca atgtgctgaa aaccgagtcg aaagtgaacg gtcaatggca aaaatatgac
     9481 gtgaaggaat caagtccatc gaaagcgaca gcttacatta gctgggcgcc ggaaccgtgg
     9541 agtctgcgtg tgcagagcac cacttctttc gacgtaagcg atgcagaggg taacgatatt
     9601 aatggttaca ctaccgtcga ttttatcagt agttggctgc ttccggtggg aacactcagc
     9661 ttcagcgttg agaacctctt cgaccgtgac tataccactg tctggggaca gcgtgcacct
     9721 ctgtactaca gcccgggtta cggccctgct tcactgtacg actacaaagg ccggggccga
     9781 acctttggtc tgaactactc agtgctgttc tgacggggat tcttttgcaa caagggtatg
     9841 ctgtaaacaa catggtcctt ggtggccatg ttgtttacag ccagtataat atacagcctt
     9901 aacaagatat tccgtctccg gaatggactg ttcaccactg aataacaata tgtctgtttt
     9961 atggatcttt gactgaaatt tatcgcatat cctcatttca ttgtccgtct gaaaactaac
    10021 cttacaa
//
LOCUS       DAZKHF020000169         9901 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2201S31241
            SAMN43267800-rid25106413.denovo.170, whole genome shotgun sequence.
ACCESSION   DAZKHF020000169 DAZKHF020000000
VERSION     DAZKHF020000169.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267800
            Sequence Read Archive: SRR30307263
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 9901)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9901)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 9901)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350581.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350581.2
            Genome Coverage       :: 151x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:21:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,652
            CDSs (total)                      :: 5,557
            Genes (coding)                    :: 5,320
            CDSs (with protein)               :: 5,320
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 237
            CDSs (without protein)            :: 237
            Pseudo Genes (ambiguous residues) :: 0 of 237
            Pseudo Genes (frameshifted)       :: 102 of 237
            Pseudo Genes (incomplete)         :: 130 of 237
            Pseudo Genes (internal stop)      :: 52 of 237
            Pseudo Genes (multiple problems)  :: 41 of 237
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9901
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267800-rid25106413.denovo.170"
                     /serotype="O 26:H11"
                     /isolate="2201S31241"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-01"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            complement(<1..615)
                     /gene="yncG"
                     /locus_tag="ACHOSC_RS16750"
                     /old_locus_tag="pgaptmp_003350"
     CDS             complement(<1..615)
                     /gene="yncG"
                     /locus_tag="ACHOSC_RS16750"
                     /old_locus_tag="pgaptmp_003350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase family protein"
                     /protein_id="HGU4549237.1"
                     /translation="MIKVYGVPGWGSTISELMLTLADIPYQFVDVSGFDHEGASRELL
                     KTLNPLCQVPTLALENDEIMTETAAIAFMVLDRRPDLAPPVGRAERQQFQRLLVWLVA
                     NVYPTFTFADYPERWASDAPEQLKKNVIEYRKSLYIWLNSQLTAEPYAFGEQLTLVDC
                     YLCTMRTWGPGHEWFQDNAQNISAIADAVCQLPKLQEVLKRNEII"
     gene            881..2380
                     /gene="ansP"
                     /locus_tag="ACHOSC_RS16755"
                     /old_locus_tag="pgaptmp_003351"
     CDS             881..2380
                     /gene="ansP"
                     /locus_tag="ACHOSC_RS16755"
                     /old_locus_tag="pgaptmp_003351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310084.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-asparagine permease"
                     /protein_id="HGU4549238.1"
                     /translation="MSKHDTDTSDQHAAKRRWLNAHEEGYHKAMGNRQVQMIAIGGAI
                     GTGLFLGAGARLQMAGPALALVYLICGLFSFFILRALGELVLHRPSSGSFVSYAREFL
                     GEKAAYVAGWMYFINWAMTGIVDITAVALYMHYWGAFGGVPQWVFALAALTIVGTMNM
                     IGVKWFAEMEFWFALIKVLAIVTFLVVGTVFLGSGQPLDGNTTGFHLITDNGGFFPHG
                     LLPALVLIQGVVFAFASIEMVGTAAGECKDPQTMVPKAINSVIWRIGLFYVGSVVLLV
                     MLLPWSAYQAGQSPFVTFFSKLGVPYIGSIMNIVVLTAALSSLNSGLYCTGRILRSMA
                     MGGSAPSFMAKMSRQHVPYAGILATLVVYVVGVFLNYLVPSRVFEIVLNFASLGIIAS
                     WAFIIVCQMRLRKAIKQGKAADVSFKLPGAPFTSWLTLLFLLSVLVLMAFDYPNGTYT
                     IAALPIIGILLVIGWFGVRKRVAEIHSTAPVVEEDEEKQEIVFKPETAS"
     gene            complement(2495..3556)
                     /gene="yncE"
                     /locus_tag="ACHOSC_RS16760"
                     /old_locus_tag="pgaptmp_003352"
     CDS             complement(2495..3556)
                     /gene="yncE"
                     /locus_tag="ACHOSC_RS16760"
                     /old_locus_tag="pgaptmp_003352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-binding-like beta-propeller repeat protein"
                     /protein_id="HGU4549239.1"
                     /translation="MHLRHLFSSRLRGSLLLGSLLVASSFSTQAAEEMLRKAVGKGAY
                     EMAYSQQENALWLATSQSRKLDKGGVVYRLDPVTLEVTQAIHNDLKPFGATINNTTQT
                     LWFGNTVNSAVTAIDAKTGEVKGRLVLDDRKRTEEVRPLQPRELVADDATNTVYISGI
                     GKESVIWVVDGENIKLKTAIQNTGKMSTGLALDSKGKRLYTTNADGELITIDTADNKI
                     LSRKKLLDDGKEHFFINISLDTARQRAFITDSKAAEVLVVDTRNGNILAKVAAPESLA
                     VLFNPARNEAYVTHRQAGKVSVIDAKSYKVVKTFDTPTHPNSLALSADGKTLYVSVKQ
                     KSTKQQEATQPDDVIRIAL"
     gene            3798..5900
                     /gene="pqqU"
                     /locus_tag="ACHOSC_RS16765"
                     /old_locus_tag="pgaptmp_003353"
     CDS             3798..5900
                     /gene="pqqU"
                     /locus_tag="ACHOSC_RS16765"
                     /old_locus_tag="pgaptmp_003353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor PqqU"
                     /protein_id="HGU4549240.1"
                     /translation="MKIFSVRQTVLPALLVLSPVVFAADEQTMIVSAAPQVVSELDTP
                     AAVSVVDGEEMRLATPRINLSESLTGVPGLQVQNRQNYAQDLQLSIRGFGSRSTYGIR
                     GIRLYVDGIPATMPDGQGQTSNIDLSSVQNVEVLRGPFSALYGNASGGVMNVTTQTGQ
                     QPPTIEASSYYGSFGSWRYGLKATGATGDGTQPGDVDYTVSTTRFTTHGYRDHSGAQK
                     NLANAKLGVRIDEASKLSLIFNSVDIKADDPGGLTKAEWKANPQQAPRAEQYDTRKTI
                     KQTQAGLRYERSLSAQDDMSVMMYAGERETTQYQSIPMAPQLNPSHAGGVITLQRHYQ
                     GIDSRWTHRGELGVPVTFTTGLNYENMSENRKGYNNFRLNSGMPEYGQKGELRRDERN
                     LMWNIDPYLQTQWQLSEKLSLDAGVRYSSVWFDSNDHYVTPGNGDDSGDASYHKWLPA
                     GSLKYAMTDAWNIYLAAGRGFETPTINELSYRADGQSGMNFGLKPSTNDTIEIGSKTR
                     IGDGLLSLALFQTDTDDEIVVDSSSGGRTTYKNAGKTRRQGAELAWDQRFAGDFRVNA
                     SWTWLDATYRSNVCNEQDCNGNRMPGIARNMGFASIGYVPEDGWYAGTEARYMGDIMA
                     DDENTAKAPSYTLVGLFTGYKYNYHNLTVDLFGRVDNLFDKEYVGSVIVNESNGRYYE
                     PSPGRNYGVGMNIAWRFE"
     gene            complement(5936..6601)
                     /gene="mcbR"
                     /locus_tag="ACHOSC_RS16770"
                     /old_locus_tag="pgaptmp_003354"
     CDS             complement(5936..6601)
                     /gene="mcbR"
                     /locus_tag="ACHOSC_RS16770"
                     /old_locus_tag="pgaptmp_003354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415967.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid/biofilm transcriptional regulator
                     McbR"
                     /protein_id="HGU4549241.1"
                     /translation="MPGMGKMKHVSLTLQVENDLKHQLSIGALKPGARLITKNLAEQL
                     GMSITPVREALLRLVSVNALSVAPAQAFTVPEVRKRQLDEINRIRYELELMAVALAVE
                     NLTPQDLAELQELLEKLQQAQEKGDMEQIINVNRLFRLAIYHRSNMPILCEMIEQLWV
                     RMGPGLHYLYEAINPAELREHIENYHLLLAALKAKDKEGCRHCLAEIMQQNIAILYQQ
                     YNR"
     gene            complement(6799..7836)
                     /gene="curA"
                     /locus_tag="ACHOSC_RS16775"
                     /old_locus_tag="pgaptmp_003355"
     CDS             complement(6799..7836)
                     /gene="curA"
                     /locus_tag="ACHOSC_RS16775"
                     /old_locus_tag="pgaptmp_003355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415966.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent curcumin/dihydrocurcumin
                     reductase"
                     /protein_id="HGU4549242.1"
                     /translation="MRQQKQRNRRWVLASRPHGAPVPENFRLEEDDVATPGEGQVLLR
                     TVYLSLDPYMRGRMSDEPSYSPPVDIGGVMVGGTVSRVVESNHPDYQPGDWVLGYSGW
                     QDYDISSGDDLVKLGDHPQNPSWSLGVLGMPGFTAYMGLLDIGQPKEGETLVVAAATG
                     PVGATVGQIGKLKGCRVVGVAGGAEKCRHAIEVLGFDVCLDHHADDFAEQLVKACPKG
                     IDIYYENVGGKVFDAVLPLLNTSARIPVCGLVSSYNATELPPGPDRLPLLMATVLKKR
                     IRLQGFIIAQDYGHRIHEFQKEMGQWVKEDKIHYHEDITDGLENAPQTFIGLLKGKNF
                     GKVVIRVAGDD"
     gene            8017..8535
                     /gene="mddA"
                     /locus_tag="ACHOSC_RS16780"
                     /old_locus_tag="pgaptmp_003356"
     CDS             8017..8535
                     /gene="mddA"
                     /locus_tag="ACHOSC_RS16780"
                     /old_locus_tag="pgaptmp_003356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-methionine sulfoximine/L-methionine sulfone
                     acetyltransferase"
                     /protein_id="HGU4549243.1"
                     /translation="MSIRFARKADCAAIAEIYNHAVLYTAAIWNDQTVDADNRIAWFE
                     ARTIAGYPVLVSEEDGVVTGYASFGDWRSFDGFRHTVEHSVYVHPDHQGKGLGRKLLS
                     RLIDEARDCGKHVMVAGIESQNQASLHLHQSLGFVVTAQMPQVGTKFGRWLDLTFMQL
                     QLDERTEPDAIG"
     gene            8532..8981
                     /gene="ydcZ"
                     /locus_tag="ACHOSC_RS16785"
                     /old_locus_tag="pgaptmp_003357"
     CDS             8532..8981
                     /gene="ydcZ"
                     /locus_tag="ACHOSC_RS16785"
                     /old_locus_tag="pgaptmp_003357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HGU4549244.1"
                     /translation="MNQSLTLAFLIAAGIGLVVQNTLMVRITQTSSTILIAMLLNSLV
                     GIVLFVSILWFKQGMAGFGELVSSVRWWTLIPGLLGSFFVFASISGYQNVGAATTIAV
                     LVASQLIGALMLDIFRSHGVPLRALFGPICGAILLVVGAWLVARRSF"
     gene            complement(8982..9215)
                     /gene="ydcY"
                     /locus_tag="ACHOSC_RS16790"
                     /old_locus_tag="pgaptmp_003358"
     CDS             complement(8982..9215)
                     /gene="ydcY"
                     /locus_tag="ACHOSC_RS16790"
                     /old_locus_tag="pgaptmp_003358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2526 family protein YdcY"
                     /protein_id="HGU4549245.1"
                     /translation="MSHLDEVIARVDAAIEESVIAHMNELLIALSDDAELSREDRYTQ
                     QQRLRTAIAHHGRKHKEDMEARHEQLTKGGTIL"
     gene            complement(9301..9474)
                     /gene="ortT"
                     /locus_tag="ACHOSC_RS16795"
                     /old_locus_tag="pgaptmp_003359"
     CDS             complement(9301..9474)
                     /gene="ortT"
                     /locus_tag="ACHOSC_RS16795"
                     /old_locus_tag="pgaptmp_003359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415962.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="orphan toxin OrtT"
                     /protein_id="HGU4549246.1"
                     /translation="MSLYQHMLVFYAVMAAIAFLITWFLSHDKKRIRFLSAFLVGATW
                     PMSFPVALLFSLF"
     gene            9669..9764
                     /gene="yncL"
                     /locus_tag="ACHOSC_RS16800"
                     /old_locus_tag="pgaptmp_003360"
     CDS             9669..9764
                     /gene="yncL"
                     /locus_tag="ACHOSC_RS16800"
                     /old_locus_tag="pgaptmp_003360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stress response membrane protein YncL"
                     /protein_id="HGU4549247.1"
                     /translation="MNVSSRTVVLINVFAAVGLFTLISMRFGWFI"
BASE COUNT         2374 a         2423 c         2429 g         2675 t
ORIGIN      
        1 aataatttca ttccttttta atacttcttg taatttcggt aactgacata cagcatcagc
       61 gatcgcgctg atattctgcg cattatcctg aaaccattca tgccccggcc cccatgtgcg
      121 catggtgcag agataacagt ccaccagcgt tagttgctca ccaaaggcat aaggttcagc
      181 ggtcaattgt gaattcagcc agatataaag tgatttgcga tattcaatta cattcttctt
      241 taactgttca ggtgcgtcag atgcccaacg ctcagggtaa tcggcgaaag tgaatgttgg
      301 atagacatta gcaaccagcc agaccaatag ccgttgaaac tgctgacgtt cggcgcgccc
      361 aaccggtggc gcaaggtccg ggcgacgatc gagaaccata aatgcaatcg ccgctgtctc
      421 cgtcataatc tcgtcatttt ccagcgccag tgtcggaacc tgacacaacg ggttcagggt
      481 tttcaataac tcgcgtgaag ctccctcgtg gtcaaaacca ctgacatcaa cgaactgata
      541 aggaatatca gccagtgtta gcatcagctc actgattgta gagccccatc cgggtacgcc
      601 atacacctta atcatatgcc ctcacaatct tttaaagatt aagtgtagga cagtacacag
      661 gttaaccagc aaaaagattt gtcatttttt cgtcagttgt attattcccc ttcaggattg
      721 taaggggatg ttttgcatcg tttatcgata acgggtagga tgctgcgccg atagttttat
      781 acccataaag aataatggtg ataactatca tcgccaggat gaataaacat tgttcatggc
      841 aacttatatg actttttcat taaagcaatc agggagagca atgagtaaac acgacaccga
      901 cacttcagat caacacgccg cgaaacgccg ctggcttaat gcccacgaag aggggtatca
      961 caaagcgatg ggcaatcgcc aggtacagat gatcgccatt ggcggcgcga ttggcaccgg
     1021 cttgttttta ggtgcaggag cccgactgca aatggcgggt ccagcattgg cactggttta
     1081 tttaatttgt ggcttgtttt cgttttttat tctgcgtgca ttgggtgagc tggtgctaca
     1141 ccgcccttcc agtggcagtt ttgtctctta tgcccgtgag tttttgggtg agaaagccgc
     1201 ttatgttgct ggctggatgt acttcatcaa ctgggcgatg acggggattg ttgatattac
     1261 cgccgtcgct ctgtatatgc attactgggg tgcgtttggc ggcgtgccgc agtgggtctt
     1321 tgcgctcgct gcacttacca tcgttggcac catgaatatg atcggtgtga aatggtttgc
     1381 ggagatggag ttctggtttg cgcttattaa agtgctcgcc attgtgacct ttttggtcgt
     1441 gggtacagtg ttcctcggta gtggtcagcc gctggatggc aacaccactg gctttcattt
     1501 aattaccgat aatggcggct tcttccccca cggtttgctg cctgctctgg tgttgattca
     1561 gggcgtagtg tttgcttttg cctccattga aatggtgggt acagctgccg gagaatgtaa
     1621 agatccgcag accatggtgc ctaaagcgat taacagcgtg atttggcgta ttggcctgtt
     1681 ttacgtcggc tccgtggtgt tgctggttat gctattgccg tggagcgcgt atcaggcggg
     1741 gcaaagtccg ttcgtgacgt ttttctctaa actgggtgtg ccatatatcg gcagcattat
     1801 gaacattgtg gtgctgaccg ctgccctctc cagcctgaac tcaggtctgt actgcaccgg
     1861 acgtattctg cgctcaatgg cgatgggcgg ttccgcaccg agttttatgg cgaaaatgag
     1921 tcgtcagcat gtgccgtatg ccgggattct ggcgacacta gttgtgtatg tcgtcggcgt
     1981 attcctcaac tatctggtgc cgtcgcgcgt atttgagatt gtgttgaact tcgcgtcgct
     2041 gggaatcatc gcttcatggg cgtttatcat cgtgtgccag atgcgcctgc gtaaagcaat
     2101 taaacaaggc aaagcagcgg atgtcagttt taaactgcct ggcgcgccct tcacttcctg
     2161 gctgacatta ctgtttttac tgagtgtcct ggtgctgatg gcgttcgatt acccgaacgg
     2221 gacttatact atcgcggcgc tgccgattat cggtattctg ctggttatag gctggtttgg
     2281 tgtgcgcaaa cgcgttgctg aaattcacag cactgcgcca gtcgtcgagg aagatgaaga
     2341 aaaacaggaa attgtgttta agcctgaaac ggcgagctaa ttgcaaaatt gcccggctac
     2401 gtgccgggca aatttagatt actgacgaag gtgtgccgca tgcggcgtga taaaagtcag
     2461 gcaagtctct ccgttttcac cctcgccaca tctattacag cgcaatacga atcacatcgt
     2521 ccggctgggt agcttcctgc tgtttagtgg atttttgttt cacactgaca tacagcgttt
     2581 tgccatcggc agacagcgcc aggctgtttg gatgagtcgg cgtatcgaac gttttcacca
     2641 ctttatagct tttcgcgtca atcacactga ctttacctgc ctgacgatgc gttacgtagg
     2701 cttcattacg cgctgggtta aacagcacag ccagtgattc tggtgccgca accttcgcca
     2761 gaatattgcc attacgggta tcgaccacta acacttctgc ggctttagaa tcggtgataa
     2821 atgcacgctg cctggcggta tcaaggctaa tgttgataaa gaagtgctct ttgccgtcat
     2881 ccagcagctt tttacggctg aggattttat tgtcggcggt gtcgatggta atcaattcgc
     2941 cgtcagcgtt agtggtgtaa agacgtttgc ctttgctatc cagcgccaga ccggtactca
     3001 ttttaccggt gttctggatg gcggttttca gtttgatatt ctcgccatca acgacccaaa
     3061 tcacgctctc tttaccaata ccactgatgt aaacggtgtt cgtggcatca tcagctacca
     3121 gctcacgcgg ttgcagcggg cgcacctctt ccgtgcgctt acgatcatcc agcaccagac
     3181 ggcctttcac ctcgcccgtt ttggcatcta tcgccgtgac cgcgctgttt acggtgttac
     3241 caaaccacaa cgtctgagtc gtgttattga tggtggcacc aaacggcttg agatcgttat
     3301 ggatcgcctg cgtcacttcc agagtgaccg gatcaagacg ataaaccacg ccacctttat
     3361 ccagtttgcg gctttgcgaa gtggcgagcc acagtgcgtt ttcttgctgg ctataagcca
     3421 tttcgtaggc acctttacct accgctttac gcagcatttc ttctgcggcc tgcgtactga
     3481 atgatgaagc aacaagcaat gaacctaaca gtaatgaacc acgcaggcgc gatgaaaaca
     3541 gatgacgtaa atgcatgacg actccctttg atgaaccgat atttttgctg cttcacaatg
     3601 cttcgctaag cgtaatcatc gttacgctct tgttataaat gggaatggta atcattattt
     3661 tctcaaatgt ggagcatttc ttgccgttac gactacaatt ttctcaatta cttaacgtat
     3721 cgtgccgttt cgttttccat aaatttacaa tttcattacc ataacggcgt tttcccattc
     3781 ttaacctggc ttttttcatg aagatttttt ccgtccgaca gaccgttttg cccgcactgc
     3841 ttgtcctttc ccccgttgtt tttgccgctg atgaacagac tatgattgtc agtgccgcac
     3901 cgcaggtggt ttcagaactg gataccccag cagcagtaag cgtggtggat ggcgaggaga
     3961 tgcgcctggc aacaccgcgc attaacttgt ccgaatcact gaccggcgtg cctggtttgc
     4021 aggtacaaaa ccggcagaac tatgcgcaag atttacagct gtcgattcgc ggatttggct
     4081 cccgctccac ttacggtatt cgcggtattc gcctgtatgt ggacggtatt cccgccacca
     4141 tgcccgacgg gcaagggcaa acatccaaca tcgatttaag cagtgtgcaa aatgtggaag
     4201 tgctgcgtgg ccccttctct gccctgtatg gcaacgcgtc tggtggggta atgaatgtca
     4261 ccacccagac cggacaacag ccaccaacca ttgaagccag tagttactac ggcagttttg
     4321 gcagctggcg ctatgggctg aaagcaacgg gcgcaacggg agacggcaca cagcctggcg
     4381 atgtcgatta caccgtctca accacgcgtt ttacgaccca cggctatcgt gaccatagtg
     4441 gcgcacagaa aaatttagct aatgccaaac tgggcgtacg cattgatgaa gccagcaaat
     4501 taagtctgat tttcaatagt gtggatatca aagcagatga cccaggtggg ctaaccaaag
     4561 cagaatggaa ggctaatcca caacaagcgc ctcgtgcaga acagtacgac acgcgaaaaa
     4621 ccatcaagca aactcaggct gggttgcgct atgagcgtag cctgagcgcg caagatgata
     4681 tgagtgtgat gatgtatgcc ggagagcgag aaacgaccca gtaccagtca atcccgatgg
     4741 ccccccaact taacccgtca catgcgggcg gcgtgattac cctgcaacgc cattaccagg
     4801 ggatagacag ccgctggaca caccgtggtg aactgggcgt tccggtcacg ttcactaccg
     4861 gcctgaacta cgaaaacatg agtgaaaacc gcaagggcta caataacttc cgcctgaata
     4921 gcggcatgcc ggagtacggg caaaaaggtg agttgcgtcg cgacgaacgc aatctgatgt
     4981 ggaacatcga tccctattta cagacgcagt ggcagctgag cgaaaaactg tcgctggatg
     5041 ctggcgtgcg ctacagctcc gtgtggtttg attccaacga ccattacgtt actccgggta
     5101 acggcgatga cagcggtgat gccagttatc ataaatggct acctgccggt tcgttaaaat
     5161 atgcaatgac cgatgcctgg aatatctatc tggcagccgg gcgaggtttt gaaacgccga
     5221 cgattaatga gctgtcttat cgtgctgatg ggcaaagcgg tatgaacttt ggtttaaaac
     5281 catccaccaa cgatacaatt gagatcggca gtaaaacgcg tattggtgat gggctgctta
     5341 gtctcgcatt gtttcagacc gacactgatg atgaaattgt tgtcgatagc agtagcggtg
     5401 ggcgtacgac ttacaaaaat gccggaaaga cccgtcgtca aggcgctgaa ctggcatggg
     5461 atcaacgttt cgcaggagat tttcgcgtaa acgcgtcctg gacctggctt gatgcgacct
     5521 atcgcagcaa tgtttgcaat gaacaggatt gtaacggtaa tcggatgcca gggatcgccc
     5581 gtaatatggg ctttgcgtcg ataggttatg taccggaaga tggttggtat gcaggcacgg
     5641 aagcgcgtta tatgggcgat attatggcag atgatgaaaa tacggcaaaa gcgccgtctt
     5701 atactctcgt cggcttattc accgggtata aatacaatta ccacaattta actgtggatt
     5761 tatttggtcg tgtcgataat ttattcgata aagaatacgt tggttctgtc attgtcaatg
     5821 agtcaaacgg gcgatattac gaaccttcgc ccggacgaaa ttatggtgtc ggcatgaata
     5881 ttgcgtggag atttgagtaa ttaccttgat gcccggtatt tgccgggcat ttactttaac
     5941 gattgtattg ctggtataaa atagcaatat tttgttgcat aatttcagca agacaatgtc
     6001 tgcatccctc tttgtctttt gcttttaacg cagcgagtaa cagatgatag ttttctatat
     6061 gctcccgtaa ttccgctgga ttaatcgctt catagagata atgtaaacca ggccccatcc
     6121 tgacccacag ttgctcaatc atctcacaca ggatgggcat atttgagcga tgataaatcg
     6181 ctaagcgaaa tagcctgttt acattaatga tttgttccat gtcgcccttt tcttgcgcct
     6241 gctgtaattt ctcgagcaat tcctggagtt ccgccaggtc ttgcggggtg aggttttcaa
     6301 cagccagagc aactgccatt aattccagtt cgtagcggat ccgattgatt tcgtctaatt
     6361 gacgtttcct cacttccgga actgtaaatg cttgtgcagg tgcgacagaa agcgcattca
     6421 ccgaaaccag acgtaataat gcttcacgca caggtgtaat actcatacct aattgctccg
     6481 ccagattttt agtaatcagg cgtgcgccag gttttaatgc gccaatacta agctgatgtt
     6541 tcaggtcgtt ctcaacctgc agagtcaaac tgacatgttt catttttccc attccaggca
     6601 ttttaattcc ttcgcgtgtt ttctgtccaa ccctgcctgc acggcagaag gagttcacct
     6661 ggtgtattaa agtgaataat cataaaccac ccacctttgt tgttattagc agcagaacaa
     6721 tgacgtggtt agttaattaa agctgaaaat aagctttttg cctggatttt tatggcggct
     6781 tacgccgccg ttgaaacttt aatcatcacc cgcgacgcgg atcaccactt tgccgaagtt
     6841 tttacccttc agcaggccga taaacgtctg tggcgcattc tctaaaccat cagtaatatc
     6901 ttcgtggtag tggattttat cctctttcac ccattgcccc atctccttct gaaactcatg
     6961 gatgcggtga ccataatcct gagcgataat aaacccttgc aagcgaatac gttttttcag
     7021 caccgttgcc atcaacaggg gtaaacgatc cggaccgggt ggtagctctg tagcgttata
     7081 gctgctcact aagccacaaa cgggaatgcg cgcagatgta ttaagtaatg gtagcactgc
     7141 atcaaatacc ttaccgccca cgttttcata atagatatca ataccttttg ggcacgcttt
     7201 caccagttgt tcggcaaaat catccgcgtg gtgatcaaga caaacatcga agcccagcac
     7261 ctcgatagca tggcggcatt tttccgcgcc accggcaacc cccaccaccc tgcaaccttt
     7321 aagtttgccg atttgcccca ccgttgctcc cacaggacct gtcgccgcag ctaccaccaa
     7381 cgtttcgccc tctttaggct gaccgatatc cagcagcccc atataagcgg taaagcctgg
     7441 catccccagc acacccagcg accacgatgg attttgcgga tgatcgccaa gtttcaccag
     7501 atcatcacca ctggatatgt catagtcctg ccatccactg tagcccagca cccagtcgcc
     7561 aggctgataa tcaggatgat tcgactccac aacacggctc accgtgccgc cgaccatcac
     7621 gccgccaata tcaacaggcg gtgaataaga tggctcatcg ctcatacgtc cacgcatata
     7681 cgggtccagg gacaaataaa ctgtgcgcag taacacctgt ccttcacccg gtgtggcgac
     7741 atcatcttct tcaagacgga aattctccgg aaccggtgcg ccatgtggac gcgaggccag
     7801 aacccaacgt cgattacgct gcttttgttg cctcataatg ctctcctgtg cttttggcat
     7861 gaatccatca atagtagcag gctcacggga ttggttttgt ttgcagccgt cacatcccct
     7921 ctgcaacacg caataatttc atcgaggaat ccgttgcttc gggctgtttt cgatagtatt
     7981 gataacatca tcatcattat tttatcgagg ccagacatgt ccatccgttt tgcccgcaaa
     8041 gccgattgtg ctgccattgc ggaaatttat aaccacgccg tgttgtatac ggcggctatc
     8101 tggaatgacc aaacggttga tgcagataac cgcattgcct ggtttgaagc gcggaccata
     8161 gcaggttatc cggtgctggt gagtgaagaa gatggcgtag tgacgggata tgcctcgttt
     8221 ggcgactggc gtagtttcga tggttttcgc cataccgtgg aacattcggt ttatgtccat
     8281 cccgatcatc agggcaaagg tctggggcgt aaattgttaa gccgattgat tgatgaagcg
     8341 cgggattgcg ggaagcatgt catggtcgcc gggatcgaat cgcaaaatca ggcctcgctg
     8401 catctgcacc agtcgctggg atttgtcgtc accgcacaaa tgccgcaggt gggcactaaa
     8461 tttgggcgct ggctggatct gacatttatg cagttgcaac tcgacgaacg cactgaaccg
     8521 gacgcgattg gatgaatcag tcgctcaccc ttgctttttt gattgccgcc ggaattggtc
     8581 tggtagtcca gaacacgctg atggtacgca tcacccagac ctcctctacc attctcatcg
     8641 ccatgttgct gaactcactg gtggggattg tgctctttgt ttctatttta tggtttaaac
     8701 aaggcatggc ggggtttggc gaactggtgt ccagcgtgcg ctggtggaca cttatacccg
     8761 gcttgctggg atcgtttttt gtcttcgcca gtatcagcgg atatcagaat gtgggagcgg
     8821 cgacaactat tgcagtactg gtcgccagcc agctaattgg cgctctgatg ctggatatat
     8881 tccgcagtca tggcgtacca ctacgggcgt tgttcggccc aatttgtggc gcaattttac
     8941 ttgtggttgg cgcctggttg gtagccagac gctcatttta atcagaggat ggtgccgcct
     9001 ttggttaact gttcgtggcg cgcttccata tcttctttat gcttgcgacc gtgatgagca
     9061 atagcggtgc gcaaacgctg ctgctgggtg tagcgatctt cccgacttaa ctccgcgtca
     9121 tcgctcaggg cgattaataa ttcgttcata tgggcaatga cgctctcttc aatcgcggca
     9181 tccacgcgcg cgatgacttc atccagatgc gacatacttt ctccttaacc acttgtgaaa
     9241 tttatcgatt catccaaatg atataagtct gcgtggtgcc gtcaagccag tgagtaagat
     9301 ttaaaacagt gaaaacaaca gcgccaccgg gaaactcatc ggccatgttg cccccaccag
     9361 aaaggcgctt aagaaacgga tgcgtttctt atcgtgagaa agaaaccagg tgataagaaa
     9421 tgcgattgct gccataaccg cataaaaaac aagcatatgt tgatagagag acatgtacgg
     9481 gcgtccagcc agatgaataa aacgcgcgca aatatgcgtc attttttgac gtgcatcaag
     9541 ttttttcacc agtttgagaa aaaatcatca aacagcaact tgcccttatc catcttcaga
     9601 atgagtatta ttgcggattt tcttaacccg tactatacac ataggggcaa tgataaaagg
     9661 tggcaaaaat gaatgtttcc agtagaactg tagtactgat aaatgtcttt gctgctgttg
     9721 gtttgtttac tcttatctct atgagatttg gctggtttat ttgatgtagt gattttgcaa
     9781 gcagcacgta ctgtcagaga aatcaactct ctgacagtac gcaagatctt ctcatactct
     9841 ccaggtttac aacattatcc ttaatattta cttctttttg ctggcatcta cattgaaatg
     9901 a
//
LOCUS       DAZKHF020000170         9868 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2201S31241
            SAMN43267800-rid25106413.denovo.171, whole genome shotgun sequence.
ACCESSION   DAZKHF020000170 DAZKHF020000000
VERSION     DAZKHF020000170.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267800
            Sequence Read Archive: SRR30307263
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 9868)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9868)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 9868)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350581.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350581.2
            Genome Coverage       :: 151x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:21:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,652
            CDSs (total)                      :: 5,557
            Genes (coding)                    :: 5,320
            CDSs (with protein)               :: 5,320
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 237
            CDSs (without protein)            :: 237
            Pseudo Genes (ambiguous residues) :: 0 of 237
            Pseudo Genes (frameshifted)       :: 102 of 237
            Pseudo Genes (incomplete)         :: 130 of 237
            Pseudo Genes (internal stop)      :: 52 of 237
            Pseudo Genes (multiple problems)  :: 41 of 237
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9868
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267800-rid25106413.denovo.171"
                     /serotype="O 26:H11"
                     /isolate="2201S31241"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-01"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            136..351
                     /gene="bdm"
                     /locus_tag="ACHOSC_RS16805"
                     /old_locus_tag="pgaptmp_003361"
     CDS             136..351
                     /gene="bdm"
                     /locus_tag="ACHOSC_RS16805"
                     /old_locus_tag="pgaptmp_003361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310112.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biofilm-dependent modulation protein"
                     /protein_id="HGU4549248.1"
                     /translation="MFTYYQAENSTAEPALVNAIEQGLRAEHGVVTEDDILMELTKWV
                     EASDNDILSDIYQQTINYVVSGQHPTL"
     gene            453..590
                     /gene="sra"
                     /locus_tag="ACHOSC_RS16810"
                     /old_locus_tag="pgaptmp_003362"
     CDS             453..590
                     /gene="sra"
                     /locus_tag="ACHOSC_RS16810"
                     /old_locus_tag="pgaptmp_003362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stationary-phase-induced ribosome-associated
                     protein"
                     /protein_id="HGU4549249.1"
                     /translation="MKSNRQARHILGLDHKISNQRKIVTEGDKSSVVNNPTGRKRPAE
                     K"
     gene            747..2444
                     /gene="maeA"
                     /locus_tag="ACHOSC_RS16815"
                     /old_locus_tag="pgaptmp_003363"
     CDS             747..2444
                     /gene="maeA"
                     /locus_tag="ACHOSC_RS16815"
                     /old_locus_tag="pgaptmp_003363"
                     /EC_number="1.1.1.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707611.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malate dehydrogenase"
                     /protein_id="HGU4549250.1"
                     /translation="MEPKTKKQRSLYIPYAGPVLLEFPLLNKGSAFSMEERRNFNLLG
                     LLPEVVETIEEQAERAWIQYQGFKTEIDKHIYLRNIQDTNETLFYRLVNNHLDEMMPV
                     IYTPTVGAACERFSEIYRRSRGVFISYQNRHNMDDILQNVPNHNIKVIVVTDGERILG
                     LGDQGIGGMGIPIGKLSLYTACGGISPAYTLPVVLDVGTNNQQLLNDPLYMGWRNPRI
                     TDDEYYEFVDEFIQAVKQRWPDVLLQFEDFAQKNAMPLLNRYRNEICSFNDDIQGTAA
                     VTVGTLIAASRAAGGQLSEKKIVFLGAGSAGCGIAEMIIAQTQREGLSEEAARQKVFM
                     VDRFGLLTDKMPNLLPFQTKLVQKRENLSDWDTDSDVLSLLDVVRNVKPDILIGVSGQ
                     TGLFTEEIIREMHKHCPRPIVMPLSNPTSRVEATPQDIIAWTEGNALVATGSPFNPVV
                     WKDKIYPIAQCNNAFIFPGIGLGVIASGASRITDEMLMSASETLAQYSPLVLNGEGLV
                     LPELKDIQKVSRAIAFAVGKMAQQQGVAVKTSAEALQQAIDDNFWQAEYRDYRRTSI"
     gene            2578..3588
                     /gene="adhP"
                     /locus_tag="ACHOSC_RS16820"
                     /old_locus_tag="pgaptmp_003364"
     CDS             2578..3588
                     /gene="adhP"
                     /locus_tag="ACHOSC_RS16820"
                     /old_locus_tag="pgaptmp_003364"
                     /EC_number="1.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310109.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase AdhP"
                     /protein_id="HGU4549251.1"
                     /translation="MKAAVVTKDHHVDVTDKTLRSLKHGEALLKMECCGVCHTDLHVK
                     NGDFGDKTGVILGHEGIGVVAEVGPGVTSLKPGDRASVAWFYEGCGHCEYCNSGNETL
                     CRSVKNAGYSVDGGMAEECIVVADYAVKVPDGLDSAAASSITCAGVTTYKAVKLSKIR
                     PGQWIAIYGLGGLGNLALQYAKNVFNAKVIAIDVNDEQLKLATEMGADLAINSRTEDA
                     AKIVQEKTGGAHAAVVTAVAKAAFNSAVDAVRAGGRVVAVGLPPESMSLDIPRLVLDG
                     IEVVGSLVGTRQDLTEAFQFAAEGKVVPKVALRPLADINTIFTEMEEGKIRGRMVIDF
                     RR"
     gene            3735..4019
                     /locus_tag="ACHOSC_RS16825"
                     /old_locus_tag="pgaptmp_003365"
     CDS             3735..4019
                     /locus_tag="ACHOSC_RS16825"
                     /old_locus_tag="pgaptmp_003365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415994.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HigA family addiction module antitoxin"
                     /protein_id="HGU4549252.1"
                     /translation="MKMANHPRPGDIIQESLDELNVSLREFARAMEIAPSTASRLLTG
                     KAALTPEMAIKLSVVIGSSPQMWLNLQNAWSLAEAEKTVDVSRLRRLVTQ"
     gene            complement(4070..4723)
                     /gene="fdnI"
                     /locus_tag="ACHOSC_RS16830"
                     /old_locus_tag="pgaptmp_003366"
     CDS             complement(4070..4723)
                     /gene="fdnI"
                     /locus_tag="ACHOSC_RS16830"
                     /old_locus_tag="pgaptmp_003366"
                     /EC_number="1.17.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase-N subunit gamma"
                     /protein_id="HGU4549253.1"
                     /translation="MSKSKMIVRTKFIDRACHWTVVICFFLVALSGISFFFPTLQWLT
                     QTFGTPQMGRILHPFFGIAIFVALMFMFVRFVHHNIPDKKDIPWLLNIVEVLKGNEHK
                     VADVGKYNAGQKMMFWSIMSMIFVLLVTGVIIWRPYFAQYFPMQVVRYSLLIHAAAGI
                     ILIHAILIHMYMAFWVKGSIKGMIEGKVSRRWAKKHHPRWYREIEKAEAKKESEEGI"
     gene            complement(4716..5600)
                     /gene="fdnH"
                     /locus_tag="ACHOSC_RS16835"
                     /old_locus_tag="pgaptmp_003367"
     CDS             complement(4716..5600)
                     /gene="fdnH"
                     /locus_tag="ACHOSC_RS16835"
                     /old_locus_tag="pgaptmp_003367"
                     /EC_number="1.17.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase N subunit beta"
                     /protein_id="HGU4549254.1"
                     /translation="MAMETQDIIKRSATNSITPPSQVRDYKAEVAKLIDVSTCIGCKA
                     CQVACSEWNDIRDEVGHCVGVYDNPADLSAKSWTVMRFSETEQNGKLEWLIRKDGCMH
                     CEDPGCLKACPSAGAIIQYANGIVDFQSENCIGCGYCIAGCPFNIPRLNKEDNRVYKC
                     TLCVDRVSVGQEPACVKTCPTGAIHFGTKKEMLELAEQRVAKLKARGYEHAGVYNPEG
                     VGGTHVMYVLHHADQPELYHGLPKDPKIDTSVSLWKGALKPLAAAGFIATFAGLIFHY
                     IGIGPNKEVDDDEEDHHE"
     gene            complement(5613..8660)
                     /gene="fdnG"
                     /locus_tag="ACHOSC_RS16840"
                     /old_locus_tag="pgaptmp_003368"
     CDS             complement(5613..8660)
                     /gene="fdnG"
                     /locus_tag="ACHOSC_RS16840"
                     /old_locus_tag="pgaptmp_003368"
                     /EC_number="1.17.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009326 - formate dehydrogenase complex
                     [Evidence IEA];
                     GO_function: GO:0008863 - formate dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0043546 - molybdopterin cofactor binding
                     [Evidence IEA];
                     GO_function: GO:0047111 - formate dehydrogenase
                     (cytochrome-c-553) activity [Evidence IEA];
                     GO_process: GO:0015942 - formate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_except=(pos:complement(8073..8075),aa:Sec)
                     /transl_table=11
                     /product="formate dehydrogenase-N subunit alpha"
                     /protein_id="HGU4549255.1"
                     /translation="MDVSRRQFFKICAGGMAGTTVAALGFAPKQALAQARNYKLLRAK
                     EIRNTCTYCSVGCGLLMYSLGDGAKNAREAIYHIEGDPDHPVSRGALCPKGAGLLDYV
                     NSENRLRYPEYRAPGSDKWQRISWEEAFSRIAKLMKADRDANFIEKNEQGVTVNRWLS
                     TGMLCASGASNETGMLTQKFARSLGMLAVDNQARVUHGPTVASLAPTFGRGAMTNHWV
                     DIKNANVVMVMGGNAAEAHPVGFRWAMEAKNNNDATLIVVDPRFTRTASVADIYAPIR
                     SGTDITFLSGVLRYLIENDKINAEYVKHYTNASLLVRDDFAFEDGLFSGYDAEKRQYD
                     KSSWNYQFDENGYAKRDETLTHPRCVWNLLKAHVSRYTPDVVENICGTPKADFLKVCE
                     VLASTSAPDRTTTFLYALGWTQHTVGAQNIRTMAMIQLLLGNMGMAGGGVNALRGHSN
                     IQGLTDLGLLSTSLPGYLTLPSEKQVDLQSYLEANTPKATLAGQVNYWSNYPKFFVSL
                     MKSFYGDAAQKENNWGYDWLPKWDQTYDVIKYFNMMDEGKVTGYFCQGFNPVASFPDK
                     NKVVSCLSKLKYMVVIDPLVTETSTFWQNHGESNDVDPASIQTEVFRLPSTCFAEEDG
                     SIANSGRWLQWHWKGQDAPGEARNDGEILAGIYHHLRELYQAEGGKGVEPLMKMSWNY
                     KQPHEPQSDEVAKENNGYALEDLYDANGVLIAKKGQLLSSFAHLRDDGTTASSCWIYT
                     GSWTEQGNQMANRDNSDPSGLGNTLGWAWAWPLNRRVLYNRASADINGKPWDPKRMLI
                     QWNGSKWTGNDIPDFGNAAPGTPTGPFIMQPEGMGRLFAINKMAEGPFPEHYEPIETP
                     LGTNPLHPNVVSNPVVRLYEQDALRMGKKEQFPYVGTTYRLTEHFHTWTKHALLNAIA
                     QPEQFVEISETLAAAKGINNGDRVTVSSKRGFIRAVAVVTRRLKPLNVNGQQVETVGI
                     PIHWGFEGVARKGYIANTLTPNVGDANSQTPEYKAFLVNIEKA"
     gene            8892..9773
                     /gene="yddG"
                     /locus_tag="ACHOSC_RS16845"
                     /old_locus_tag="pgaptmp_003369"
     CDS             8892..9773
                     /gene="yddG"
                     /locus_tag="ACHOSC_RS16845"
                     /old_locus_tag="pgaptmp_003369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415990.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic amino acid efflux DMT transporter YddG"
                     /protein_id="HGU4549256.1"
                     /translation="MTRQKATLIGLIAIVLWSTMVGLIRGVSEGLGPVGGAAAIYSLS
                     GLLLIFTVGFPRIRQIPKGYLLAGSLLFVSYEICLALSLGYAATRHQAIEVGMVNYLW
                     PSLTILFAILFNGQKTNWLIVPGLLLALVGVCWVLGGENGLHYDEIINNITTSPLSYF
                     LAFIGAFIWAAYCTVTNKYARGFNGITVFVLLTGASLWVYYFLTPQPEMVFSTPVMIK
                     LISAAFTLGFAYAAWNVGILHGNVTIMAVGSYFTPVLSSALAAVLLSAPLSFSFWQGA
                     LMVCGGSLLCWLATRRG"
BASE COUNT         2397 a         2585 c         2491 g         2395 t
ORIGIN      
        1 tgaggttaaa atataaaaat catcaaccag gactaatctt aacaacgaag cggcaaatat
       61 ttgccatgct gaatgtgctt tataaatctg cgatccgtag cagacaccat aaatacacag
      121 acacggagaa tcactatgtt tacttattat caggcagaaa attcaacagc agaacccgct
      181 ctggttaatg ccattgagca aggccttcgg gcagagcacg gtgttgtcac tgaagatgac
      241 attctcatgg agctgaccaa atgggtggaa gcttctgata acgacatcct cagtgatatc
      301 taccagcaaa cgatcaatta tgtggtcagt ggccagcacc ctacgcttta aggtgctatg
      361 cttgatcggc aacctaattt aggggtttag cacgtgtttc ttcgctacgg cgatgttgtc
      421 cttaaaacta gcaacaggat tgaggagtta aaatgaaatc gaaccgtcag gcacgtcata
      481 ttcttggact ggaccataaa atttctaacc agcgcaaaat agttaccgaa ggtgacaaat
      541 ccagcgtggt aaataatcca accggcagaa aacgccccgc tgaaaagtaa ttcataacca
      601 tcagtcctca atgacgatta aacaccattg cctgcgcaat ggtgtttttg tttttatctg
      661 ctctatactt ggggccgacg ccctggcggt aaagcaaaga cgataaaagc gtgccaggga
      721 tggatattca aaaaagagtg agtgacatgg aaccaaaaac aaaaaaacag cgttcgcttt
      781 atatccctta cgctggccct gtactgctgg aatttccgtt gttgaataaa ggcagtgcct
      841 tcagcatgga agaacgccgt aacttcaacc tgctggggtt actgccggaa gtggtcgaaa
      901 ccatcgaaga acaagcggaa cgagcatgga tccagtatca gggattcaaa accgaaatcg
      961 acaaacacat ctacctgcgt aacatccagg acaccaacga aaccctcttc taccgtctgg
     1021 taaacaatca tcttgatgag atgatgcctg ttatttatac cccaaccgtc ggcgcagcct
     1081 gtgagcgttt ttctgagatc taccgccgtt cacgcggcgt gtttatctct taccagaacc
     1141 ggcacaatat ggacgatatt ctgcaaaacg tgccgaacca taatattaaa gtgattgtgg
     1201 tgactgacgg tgaacgtatt ctggggcttg gtgaccaggg catcggcggg atgggcattc
     1261 cgatcggtaa actgtcgctc tataccgcct gtggcggcat cagcccggcg tatacccttc
     1321 cggtggtgct ggatgtcgga acgaacaacc aacagctgct taacgatccg ctgtatatgg
     1381 gctggcgtaa tccacgtatc actgacgatg aatactatga attcgttgat gaatttatcc
     1441 aggctgtgaa acaacgctgg ccggacgtgc tgttgcagtt tgaagacttt gctcaaaaaa
     1501 atgcgatgcc gttacttaac cgctatcgca atgaaatttg ttcttttaac gatgacattc
     1561 agggcactgc ggcagtaaca gtcggcacac tgatcgcagc cagccgcgcg gcaggtggtc
     1621 agttaagcga gaaaaaaatc gtcttccttg gcgcaggttc agcgggatgc ggcattgccg
     1681 aaatgatcat cgcccagacc cagcgcgaag gattaagcga ggaagcagcg cggcagaaag
     1741 tctttatggt cgatcgcttt ggcctgctga cggacaagat gccgaacctg ctacctttcc
     1801 agaccaaact ggtgcagaag cgcgaaaacc tcagtgactg ggataccgac agcgatgtgt
     1861 tgtcactgct ggatgtggtg cgcaatgtaa aaccagatat tctgattggc gtctcaggac
     1921 agaccgggct gtttacggaa gagatcattc gtgagatgca taaacactgt ccgcgtccga
     1981 tcgtgatgcc gctgtctaac ccgacgtcac gcgtggaagc cacaccgcag gacattatcg
     2041 cctggaccga aggtaacgcg ctggtcgcca ctggcagccc gtttaatcca gtggtatgga
     2101 aagataaaat ctaccctatc gcccagtgta acaacgcctt tattttccca ggcatcgggc
     2161 tgggtgttat tgcttccggc gcgtcacgta tcaccgatga gatgctgatg tcagcaagtg
     2221 aaacgcttgc tcagtattcg ccgctggtgc tgaacggcga aggtctggta ctaccggaac
     2281 tgaaagatat tcagaaagtc tcccgcgcaa ttgcgtttgc ggttggcaaa atggcgcagc
     2341 agcaaggcgt ggcggtgaaa acgtctgccg aagctctgca acaagccatt gacgataatt
     2401 tctggcaagc cgaataccgc gactaccgcc gtacctccat ctaagcctgc gcccggtagt
     2461 gaaggctacc gggctatttc cctctccctt tttcagatct catccatact gggtagtggc
     2521 gaataaatct catttgcctc acctgctatg cagaacatca tccgaaaagg aggaactatg
     2581 aaggctgcag ttgttacgaa ggatcatcat gttgacgtta cggataaaac actgcgctca
     2641 ctgaaacatg gcgaagccct gctgaaaatg gagtgttgtg gtgtatgtca taccgatctt
     2701 catgttaaga atggcgattt tggtgacaaa actggggtaa ttctgggcca tgaaggcatc
     2761 ggtgtggtgg cagaagtggg tccaggtgtc acctcattaa aaccaggcga tcgtgccagc
     2821 gtggcgtggt tctacgaagg atgcggtcat tgcgaatact gtaacagtgg taacgaaacg
     2881 ctctgccgtt cagttaaaaa tgccggatac agcgttgatg gcgggatggc ggaagagtgc
     2941 atcgtggtcg ccgattacgc ggtaaaagtg ccagatggtc tggactcggc ggcggccagc
     3001 agcattacct gtgcgggggt caccacctac aaagccgtta aactgtcaaa aattcgtccc
     3061 ggtcagtgga ttgctatcta cggtcttggc ggtctgggta acctcgccct gcaatacgcg
     3121 aagaatgtct ttaacgccaa agtgatcgcc attgatgtca atgatgagca gttaaaactg
     3181 gcaaccgaaa tgggtgcaga tttagcgatt aactcacgca ccgaagacgc cgccaaaatt
     3241 gtgcaggaga aaaccggtgg cgctcacgct gcggtggtaa cagcagttgc taaagctgcg
     3301 tttaactcgg cagttgatgc tgtccgtgca ggcggtcgtg ttgtggctgt cggtctgccg
     3361 ccggagtcta tgagcctgga tatcccacgc cttgtgctgg atggcattga agtggtcggt
     3421 tcgctggtcg gcacgcgcca ggatctaacc gaagccttcc agtttgccgc cgaaggcaaa
     3481 gtggtgccga aagtcgccct gcgtccgtta gcggacatca acaccatctt taccgagatg
     3541 gaagaaggca aaatccgtgg ccgtatggtg attgatttcc gccgttaaga ggcctttgct
     3601 gcgagtgcca gtcctggtgt cgcagcatcg cactctctcc gacattaaat ggctaaatgg
     3661 attattgttc cttcctacgc aacaataatg atagtgttcc tgatgaagga acactactga
     3721 gagtaaagac actgatgaaa atggccaatc atccccgccc gggggacatt attcaggaat
     3781 cactggacga acttaatgtc agcctgcgcg agtttgccag agcaatggaa attgctccct
     3841 caacggcaag ccgattgctg accggaaaag cagctttgac accagaaatg gcgattaaac
     3901 tttccgtggt gatcggcagt tcgccgcaaa tgtggctgaa tctgcaaaac gcctggagtc
     3961 tggcagaggc agaaaaaacg gtggatgtgt cgcgactacg ccgtctggtt acgcaataaa
     4021 aaaatggcgc ttcttaaagc gccattttca gcttaaagtt caaaagagat tatatccctt
     4081 cttcactctc ttttttcgct tctgccttct cgatttcacg ataccagcgc ggatggtgtt
     4141 tcttcgccca gcgacggctc accttccctt cgatcatccc tttaatcgac cctttcaccc
     4201 agaatgccat atacatatgg atcaggatgg cgtggatcag gatgataccc gcagccgcgt
     4261 ggatcagcag gctgtagcga acaacctgca tcgggaagta ctgcgcaaag tacggacgcc
     4321 agataatcac cccggtcacc agcagcacga aaatcatgct catgatcgac cagaacatca
     4381 tcttttgccc ggcgttgtac ttaccgacat ccgccacttt atgctcattg cctttcaata
     4441 cttcgacaat gttcaacagc cacggaatat ctttcttatc cgggatgttg tgatgcacaa
     4501 aacgcacaaa cataaacatc agtgcgacga aaatcgcaat gccgaaaaac gggtgcaaaa
     4561 tgcgtcccat ctgcggcgta ccgaaggttt gcgtcagcca ttgcagcgtc gggaagaaga
     4621 acgaaatccc ggacagcgcc accaggaaga agcaaatcac cacggtccag tgacaggcgc
     4681 gatcaataaa tttggtgcgc acaatcattt tcgacttact catgatgatc ctcctcgtca
     4741 tcgtccactt ccttattcgg gccaataccg atgtagtgga aaatcaaccc ggcaaaagtg
     4801 gcaataaagc cagccgctgc cagcggtttc aacgcgcctt tccacaggct taccgaggtg
     4861 tcgatcttcg gatctttcgg cagaccgtga tacagctccg gctgatcggc gtgatgcagc
     4921 acgtacataa cgtgcgtacc accgacccct tccgggttgt agacgccagc atgttcgtaa
     4981 ccacgcgctt tcagtttcgc cacgcgctgt tccgccagct ccagcatctc cttcttggtg
     5041 ccgaagtgga tagccccggt cggacaggtt ttcacacaag ccggttcctg gccgacgctg
     5101 acgcgatcga cgcagagcgt gcatttatat acccggttat cctctttgtt gaggcgcgga
     5161 atattaaacg gacacccggc aatgcagtaa ccacagccga tgcagttttc cgactggaaa
     5221 tcgacaatcc cgttagcgta ctgaatgatt gcaccagcag acgggcacgc cttcaggcag
     5281 ccgggatctt cacagtgcat acagccgtct ttacggatca gccactccag cttgccgttc
     5341 tgttcggttt cgctaaagcg catcaccgtc caggacttgg cgctcagatc ggcggggtta
     5401 tcgtaaaccc cgacgcagtg ccccacttca tcacggatgt cgttccactc cgaacacgcc
     5461 acctgacagg ctttacagcc gatacaggtg gaaacgtcga taagttttgc gacttctgct
     5521 ttgtaatcac gcacctgaga aggcggcgtg atggagttag ttgcggacct tttgataatg
     5581 tcctgcgttt ccatagccat ctgttcgccc ccttacgcct tctcgatgtt gactaagaac
     5641 gctttatatt ccggcgtttg cgagtttgca tcaccgacat tcggcgtcag agtgttagcg
     5701 atataacctt tacgcgcgac accctcaaag ccccagtgga ttggaatacc caccgtttca
     5761 acctgctgac catttacatt cagtggtttc agacgacgcg ttaccacagc caccgcgcgg
     5821 ataaagccac gcttgcttga gacagtgaca cgatcgccat tattaatgcc tttcgccgcc
     5881 gccagcgttt cgctgatttc cacaaactgt tccggctgag caattgcgtt gagcaatgcg
     5941 tgcttggtcc aggtgtggaa gtgctcggtc agacgatagg tcgtacccac atacgggaac
     6001 tgctcttttt tacccatccg cagtgcgtct tgttcataca gacgaacaac cgggttagac
     6061 accacgttcg gatgcagcgg gttagtgccc agcggcgttt caatcggctc gtagtgttcc
     6121 gggaacggac cttccgccat tttgttgatg gcaaacaggc gtcccatccc ttccggctgc
     6181 atgataaacg gcccggttgg cgtacccggt gcggcattgc cgaagtcagg aatatcgtta
     6241 cccgtccact tgctgccgtt ccactggatc agcatccgtt tcggatccca cggtttaccg
     6301 ttgatatccg ccgaagcacg gttgtacagc acgcgacggt tgagcggcca cgcccaggcc
     6361 catcccagcg tattccccag accggacggg tcggagttat cgcggttagc catctggttg
     6421 ccctgctctg tccagctacc ggtgtagatc cagcaagaag atgcggttgt accgtcatca
     6481 cgcagatgcg caaagctact cagcaactga cctttcttcg caatcagcac gccattagca
     6541 tcatagagat cttccagcgc atagccgttg ttctctttag ctacttcgtc agattgcggt
     6601 tcgtgcggct gcttgtagtt ccagctcatc ttcatcagcg gttctacgcc tttaccacct
     6661 tcggcctggt acagctcgcg caggtgatgg tagatacccg ccagaatttc accgtcgtta
     6721 cgcgcttcgc ccggcgcatc ctgacctttc cagtgccact gcagccagcg accggagtta
     6781 gcaatagaac catcttcttc agcaaagcag gtcgaaggca gacggaatac ttcagtctga
     6841 atagacgccg gatcgacatc gttcgactcg ccgtggttct gccagaaggt tgaggtttca
     6901 gtcaccagcg gatcgataac caccatgtac ttcagcttgc ttaggcagct caccactttg
     6961 tttttgtccg ggaaggacgc aaccgggtta aagccctggc agaaataacc ggtgactttg
     7021 ccttcatcca tcatgttgaa atacttgatg acgtcgtagg tctggtccca cttcggcagc
     7081 cagtcatagc cccagttgtt ctctttctgc gcggcatcgc catagaaaga tttcatcagg
     7141 ctaacgaaga acttcggata gttgctccag tagttcacct gaccagccag cgtcgctttc
     7201 ggcgtgttcg cttccagata cgactgcaaa tcaacctgtt tttctgacgg cagcgtcaga
     7261 taacctggca ggctggtaga gagcagacct aagtcagtca gaccctgaat gttggagtga
     7321 ccacgcaatg cattcacgcc gccaccggcc atacccatgt taccgagcag caactggatc
     7381 atcgccatag tacggatgtt ctgcgcaccc acggtgtgct gcgtccagcc cagcgcgtac
     7441 aggaaggtgg ttgtgcgatc cggtgcgctg gtggaggcca gcacttcaca cactttcagg
     7501 aagtcggctt ttggcgtacc acagatgttt tcaacaacgt ccggcgtgta gcgggaaacg
     7561 tgcgctttca gcaggttcca cacacagcgc ggatgagtca gtgtttcatc gcgtttcgca
     7621 tagccgtttt catcgaactg atagttccag gacgatttat cgtattgccg tttttcagcg
     7681 tcgtagccgc tgaacagacc gtcttcgaaa gcaaaatcat cacgcaccag caggctggcg
     7741 ttggtgtaat gcttaacgta ttcggcgttg attttgtcgt tttcgatcag gtagcgcaaa
     7801 acgccagaca ggaacgtaat gtccgtaccg gaacgaatag gtgcgtaaat atccgccaca
     7861 gaagcggtac gcgtaaaacg aggatcgaca acgatcaagg ttgcatcgtt gttgtttttc
     7921 gcttccatcg cccagcggaa accgacggga tgcgcttcag cagcgttacc gcccattacc
     7981 attacgacgt tagcgttttt gatatccacc cagtggttgg tcatcgcacc gcgaccaaat
     8041 gttggagcaa gacttgctac cgttggtccg tgtcagacgc gcgcctggtt gtctaccgcc
     8101 agcatcccga gggagcgggc aaatttctgt gtcagcatcc cggtttcgtt gctggcaccg
     8161 gaggcacaca gcataccggt agaaagccaa cggtttaccg ttacgccctg ctcgttcttt
     8221 tcaataaagt tagcgtcacg gtcagctttc atcagctttg caatacggga gaatgcttct
     8281 tcccagctaa tgcgctgcca tttgtcagaa cctggcgcac gatattccgg gtagcgcaga
     8341 cggttttcgc tgttgacgta atccagcaaa ccggcccctt ttgggcacag cgcaccacgg
     8401 cttaccggat gatccgggtc accttcaatg tgataaatcg cttctctggc gtttttcgcg
     8461 ccatcaccca ggctatacat caatagcccg caacctacgg aacagtatgt gcaggtgtta
     8521 cggatctctt tagcgcgtaa taatttgtag tttcgcgcct gagccagtgc ttgcttcggg
     8581 gcaaagccca atgccgctac cgttgttcca gccataccgc ccgcgcagat tttaaaaaat
     8641 tgtctgcgac tgacgtccat tgctttcctc ttttttcagg ggtattactg cgcgaggaaa
     8701 ctaacagtaa aattctgaat ctttttgcgc gaaatcaaaa agaacacgtg tttgccacaa
     8761 aataatccgc ctgcgcaaat tgacctacct caatagcggt agaaaaacgc accactgcct
     8821 gacaggccag ttaaaaaaat gctataaaat tcagcttaat ttttaacggc aagagagaca
     8881 aaacagcgag catgacacga caaaaagcaa cgctcatagg gctaatagcg atcgtcctgt
     8941 ggagcacgat ggtaggattg attcgcggtg tcagcgaggg gctcggcccg gtcggcggcg
     9001 cagctgctat ctattcatta agcgggctgc tgttaatctt cacggttgga tttccgcgta
     9061 ttcggcagat tccgaaaggc tatttactcg ccgggagtct gttattcgtc agctatgaaa
     9121 tctgtctggc gctttcctta gggtatgcgg cgacccgtca tcaggcgatt gaagtgggta
     9181 tggtgaacta tctgtggccc agcctgacaa ttctctttgc cattctgttt aatggtcaga
     9241 aaaccaactg gctgattgta ccaggattat tattagccct ggtcggcgtc tgctgggtgt
     9301 taggcggtga gaatgggtta cattatgatg aaatcatcaa taatatcacc accagcccat
     9361 tgagttattt cctggcgttt attggtgcgt ttatctgggc agcctattgc acagtaacga
     9421 ataaatacgc acgcggattt aacggaatta ccgtttttgt cctgctaacg ggagcaagtc
     9481 tgtgggttta ctattttctc acgccacaac cagaaatggt atttagcacg cccgtcatga
     9541 ttaaactcat ctctgcggca tttaccttag gatttgctta tgctgcatgg aatgtcggta
     9601 tactgcatgg caatgtcacc ataatggcgg taggctcgta ttttacgccc gtactttcct
     9661 cagcgcttgc agccgtgctg ctcagcgccc cgctgtcgtt ctcattctgg caaggcgcgc
     9721 taatggtctg cggcggttcc ctgctctgct ggctggcgac acgtcgtggt taaaataatt
     9781 gccgggtcta cggcccggca gttaatcaga taaattccga caatattaaa aaataaattt
     9841 caaataccag taattcacct tatatctc
//
LOCUS       DAZKHF020000171         9849 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2201S31241
            SAMN43267800-rid25106413.denovo.172, whole genome shotgun sequence.
ACCESSION   DAZKHF020000171 DAZKHF020000000
VERSION     DAZKHF020000171.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267800
            Sequence Read Archive: SRR30307263
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 9849)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9849)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 9849)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350581.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350581.2
            Genome Coverage       :: 151x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:21:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,652
            CDSs (total)                      :: 5,557
            Genes (coding)                    :: 5,320
            CDSs (with protein)               :: 5,320
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 237
            CDSs (without protein)            :: 237
            Pseudo Genes (ambiguous residues) :: 0 of 237
            Pseudo Genes (frameshifted)       :: 102 of 237
            Pseudo Genes (incomplete)         :: 130 of 237
            Pseudo Genes (internal stop)      :: 52 of 237
            Pseudo Genes (multiple problems)  :: 41 of 237
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9849
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267800-rid25106413.denovo.172"
                     /serotype="O 26:H11"
                     /isolate="2201S31241"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-01"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            169..939
                     /gene="yjjP"
                     /locus_tag="ACHOSC_RS16850"
                     /old_locus_tag="pgaptmp_003370"
     CDS             169..939
                     /gene="yjjP"
                     /locus_tag="ACHOSC_RS16850"
                     /old_locus_tag="pgaptmp_003370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418784.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine exporter ThrE family protein"
                     /protein_id="HGU4549257.1"
                     /translation="MQTEQQRAVTRLCIQCGLFLLQHGAESALVDELSSRLGRALGMD
                     SVESSISSNAIVLTTIKDGQCLTSTRKNHDRGINMHVVTEVQHIVILAEHHLLDYKGV
                     EKRFSQIQPLRYPRWLVALMVGLSCACFCKLNNGGWDGAVITFFASTTAMYIRQLLAQ
                     RHLHPQINFCLTAFAATTISGLLLQLPTFSDTPTIAMAASVLLLVPGFPLINAVADMF
                     KGHINTGLARWAIASLLTLATCVGVVMALTIWGLRGWV"
     gene            930..1403
                     /gene="yjjB"
                     /locus_tag="ACHOSC_RS16855"
                     /old_locus_tag="pgaptmp_003371"
     CDS             930..1403
                     /gene="yjjB"
                     /locus_tag="ACHOSC_RS16855"
                     /old_locus_tag="pgaptmp_003371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418783.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine exporter"
                     /protein_id="HGU4549258.1"
                     /translation="MGVIEFLLALAQDMILAAIPAVGFAMVFNVPVRALRWCALLGAI
                     GHGSRMILMTSGLNIEWSTFMASMLVGTIGIQWSRWYLAHPKVFTVAAVIPMFPGISA
                     YTAMISAVKISQLGYSEPLMITLLTNFLTASSIVGALSIGLSIPGLWLYRKRPRV"
     gene            1510..2049
                     /gene="dnaT"
                     /locus_tag="ACHOSC_RS16860"
                     /old_locus_tag="pgaptmp_003372"
     CDS             1510..2049
                     /gene="dnaT"
                     /locus_tag="ACHOSC_RS16860"
                     /old_locus_tag="pgaptmp_003372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein DnaT"
                     /protein_id="HGU4549259.1"
                     /translation="MSSRVLTPDVVGIDALVHDHQTVLAKAEGGVVAVFANNAPAFYA
                     VTPARLAELLALEEKLARPGSDVALDDQLYQEPQAAPVAVPMGKFAMYPDWQPDADFI
                     RLAALWGVALREPVTTEELASFIAYWQAEGKVFHHVQWQQKLARSLQIGRASNGGLPK
                     RDVNTVSEPDSQIPPGFRG"
     gene            2052..2789
                     /gene="dnaC"
                     /locus_tag="ACHOSC_RS16865"
                     /old_locus_tag="pgaptmp_003373"
     CDS             2052..2789
                     /gene="dnaC"
                     /locus_tag="ACHOSC_RS16865"
                     /old_locus_tag="pgaptmp_003373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication protein DnaC"
                     /protein_id="HGU4549260.1"
                     /translation="MKNVGDLMQRLQKMMPAHIKPAFKTGEELLAWQKEQGAIRSAAL
                     ERENRAMKMQRTFNRSGIRPLHQNCSFENYRVECEGQMNALSKARQYVEEFDGNIASF
                     IFSGKPGTGKNHLAAAICNELLLRGKSVLIITVADIMSAMKDTFRNSGTSEEQLLNDL
                     SNVDLLVIDEIGVQTESKYEKVIINQIVDRRSSSKRPTGMLTNSNMEEMTKLLGERVM
                     DRMRLGNSLWVIFNWDSYRSRVTGKEY"
     gene            2838..3332
                     /gene="yjjA"
                     /locus_tag="ACHOSC_RS16870"
                     /old_locus_tag="pgaptmp_003374"
     CDS             2838..3332
                     /gene="yjjA"
                     /locus_tag="ACHOSC_RS16870"
                     /old_locus_tag="pgaptmp_003374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418780.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2501 domain-containing protein YjjA"
                     /protein_id="HGU4549261.1"
                     /translation="MMKTVKHLLCCAIAASALISTGVHAASWKDALSSAASELGNQNS
                     TTQEGGWSLASLTNLLSSGNQALSADNMNNAAGILQYCAKQKLASVTDAENIKNQVLE
                     KLGLNSEEQKEDTNYLDGIQGLLKTKDGQQLNLDNIGTTPLAEKVKTKACDLVLKQGL
                     NFIS"
     gene            3586..5877
                     /gene="opgB"
                     /locus_tag="ACHOSC_RS16875"
                     /old_locus_tag="pgaptmp_003375"
     CDS             3586..5877
                     /gene="opgB"
                     /locus_tag="ACHOSC_RS16875"
                     /old_locus_tag="pgaptmp_003375"
                     /EC_number="2.7.8.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418779.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylglycerol--membrane-oligosaccharide
                     glycerophosphotransferase"
                     /protein_id="HGU4549262.1"
                     /translation="MSELLSFALFLASVLIYAWKAGRNTWWFAATLTVLGLFVVLNIT
                     LFASDYFTGAGINDAVLYTLTNSLTGAGVSKYILPGIGIVLGLTAVFGALGWILRRRR
                     HHPHHFGYSLLALLLALGSVDASPAFRQITELVKSQSRDGDPDFAAYYKEPSKTIPDP
                     KLNLVYIYGESLERTYFDNEAFPDLTPELGALKNEGLDFSHTQQLPGTDYTIAGMVAS
                     QCGIPLFAPFEGNASASVSSFFPQNICLGDILKNSGYQNYFVQGANLRFAGKDVFLKS
                     HGFDHLYGSEELKSVVADPHYRNDWGFYDDTVLDEAWKKFEELSRSGQRFSLFTLTVD
                     THHPDGFISRTCNRKKYDFDGKPNQSFSAVSCSQENIAAFINKIKASPWFKDTVIVVS
                     SDHLAMNNTAWKYLNKQDRNNLFFVIRGDKPQQETLAVKRNTMDNGATVLDILGGDNY
                     LGLGRSSLSGQSMSEIFLNIKEKTLAWKPDIIRLWKFPKEMKEFTIDQQKNMIAFSGS
                     HFRLPLLLRVSDKRVEPLPESEYSAPLRFQLADFAPRDNFVWVDRCYKMAQLWAPELA
                     LSTDWCVSQGQLGGQQIVQHVDKTTWQGKTAFKDTVIDMARYKGNVDTLKIVDNDIRY
                     KADSFIFNVAGAPEEVKQFSGISRPESWGRWSNAQLGDEVKIEYKHPLPKKFDLVITA
                     KAYGNNASRPIPVRVGNEEQTLVLGNEVTTTTLHFDNPTDADTLVIVPPEPVSTNEGN
                     ILGHSPRKLGIGMVEIKVVEREG"
     gene            complement(6016..7038)
                     /gene="lgoD"
                     /locus_tag="ACHOSC_RS16880"
                     /old_locus_tag="pgaptmp_003376"
     CDS             complement(6016..7038)
                     /gene="lgoD"
                     /locus_tag="ACHOSC_RS16880"
                     /old_locus_tag="pgaptmp_003376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418778.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding alcohol dehydrogenase family
                     protein"
                     /protein_id="HGU4549263.1"
                     /translation="MSTMKVLICQEIKNLVWTVRERPVPGDNEALIKIKTVGICGTDI
                     HAWAGNQPFFSYPRVLGHEICGEIIESGKNFHNFNIGQQVAVIPYISCQQCPACKSGR
                     TNCCEKISVIGVHQDGGFSEYLSVPVANILPADGIDPQAAALIEPFAISAHAVRRAAI
                     APGEQVLVVGAGPIGLGAAAIAKADGAQVVVADTSPARREHVATRLELPVLDPSAEDF
                     DAQLRAQFGGSLAQKVIDATGNQHAMNNTVNLIRHGGTVVFVGLFKGELQFSDPEFHK
                     KETTMMGSRNATPEDFAKVGRLMAEGKITADMMLTHRYPFATLAETYERDVINNRELI
                     KGVITF"
     gene            7177..8082
                     /locus_tag="ACHOSC_RS16885"
                     /old_locus_tag="pgaptmp_003377"
     CDS             7177..8082
                     /locus_tag="ACHOSC_RS16885"
                     /old_locus_tag="pgaptmp_003377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000091590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FCD domain-containing protein"
                     /protein_id="HGU4549264.1"
                     /translation="MSRSQNLRHNVINQVIEDMARGNIPSPLPSQSGLAEMYNISRTT
                     VRHILQHLSACGVLTLVGKNYVIARKPEQNDGFDCITASLKEQNRLFEQAFFTMINLR
                     QLRAGESFSELQLARAAGVSPVVVREYLLKFGRYNLIQSEKRGQWSMKQFDQSYAEQL
                     FELREMLETHSLQHFLNLPDDDPRWLQAKTMLERHRTLRDSIGSNFRMFSQLDRDFHA
                     MLLSAADNIFFNQSLEIISVIFHFHYQWDESDLKQRNIIAVDEHMTILSALICRSDLD
                     AITALRNHLDTAKQSMIRSIRQHHR"
     gene            complement(8253..>9849)
                     /locus_tag="ACHOSC_RS16890"
                     /old_locus_tag="pgaptmp_003378"
     CDS             complement(8253..>9849)
                     /locus_tag="ACHOSC_RS16890"
                     /old_locus_tag="pgaptmp_003378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001680749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015385 - sodium:proton antiporter
                     activity [Evidence IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA];
                     GO_process: GO:0006885 - regulation of pH [Evidence IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="Na+/H+ antiporter"
                     /protein_id="HGU4549265.1"
                     /translation="LTRLSPVQIPLPLIQIAAGAVLAQPIFGLHVEFNPELFLLLFIP
                     PLLFAESSKIQPKELIKHSREIISLALVLVLITIFGVGYVIHLLLPNVPLIAAFALAA
                     VLSPTDAVALLGIVGKGRISKNIQEVLEGEALMNDASGLVALKFAVAVTMGTMEFSVH
                     GATIAFFVVALGGIAVGIAVTWLYGKGLLLISRYAHDEPSIQIVLMLLLPFIVYLVAE
                     HFGLSGILAAVAAGLTTSRIGVVHHAPIRLRIKAKSSWSMLEYVFNGMVFLILGLQLP
                     GIISESLIRAENDPLVDTGSLLLDVIWIYLALMVVRLGWLWLMRLYSRKRPGKHPMLF
                     AGYKTRHMLLATFAGVRGAITLAGVLSIPLLLPDGTPFPGRYLLVFLATGVILFSLVC
                     GIIILPLLLGKNVGIFSDNRQKEEEAADLFMAKMAILSVQKTEQRLLRTSTENLDNEL
                     IMETGARVIGYMRRSEDDMSENDNSLHMQRAKLERTFNLAALNARRAAIYQMRARQEV
                     SDETLAKIVSELDVLETLIMDSR"
BASE COUNT         2531 a         2561 c         2378 g         2379 t
ORIGIN      
        1 gtttgcggcg agagataatt cgctttttat caccgagctt ctcccgataa ccaggattaa
       61 tcagcgcaat tttgatctta acctaccgca aaacaaccgt gcttcatgat acaaatggat
      121 tctcattatc tgaacaatac gcagcacgtc tatgacaaag ggcgagttat gcaaactgag
      181 caacagcgag ccgtaacacg gctttgtatc cagtgtggat tatttctttt gcaacatggt
      241 gcggaaagcg cgttggttga tgagctttcc tcacgactgg gtcgggcgct gggaatggac
      301 agcgtcgaaa gttctatctc ttcgaacgcc atagtgctga caaccattaa agatgggcaa
      361 tgcctgacat cgacacgtaa aaatcacgat cgcggcatta atatgcatgt ggtgactgaa
      421 gtccagcaca ttgtgattct tgcggagcat catctgctgg attacaaagg cgtagagaaa
      481 cgatttagcc aaattcagcc attacgttac ccaagatggc tggtagcctt aatggttggc
      541 ctttcttgcg cctgtttctg taaactcaat aacggtggct gggatggtgc cgtcatcacc
      601 ttctttgcca gtacgaccgc gatgtatatc cgccagctgc tggcacaacg tcatcttcat
      661 ccacagatca acttttgcct taccgctttc gccgccacca ccatttccgg attgcttttg
      721 caactcccca ctttcagcga tacacccacc attgcgatgg ccgccagcgt tctgctgctg
      781 gtgccgggct ttccgttgat taatgccgtc gccgatatgt ttaaaggcca catcaatacc
      841 ggactggcac gctgggcgat cgccagtctg ctgacgctgg ctacctgcgt cggcgtggtg
      901 atggcactga cgatttgggg gctacgcgga tgggtgtgat cgaatttctg ttagcgttgg
      961 cgcaggatat gatcctcgcc gccattcctg cggtcggctt tgcgatggtg ttcaacgttc
     1021 ccgttcgggc gttacgctgg tgtgcgctgc ttggcgcgat tggtcatggt tcacgaatga
     1081 tcttaatgac cagcgggctg aatattgagt ggtcaacttt tatggcttct atgctggtcg
     1141 gcaccattgg tattcaatgg tcgcgctggt atctggcgca tccgaaagtg tttaccgtgg
     1201 cggccgttat ccctatgttc ccgggcatat cggcttatac cgcaatgatt tcggcggtaa
     1261 aaatcagcca gttaggttac agcgaaccgt tgatgattac cctgttaacc aactttctta
     1321 cagcttcatc gattgttggt gcgttatcca tcggtctttc cattcctgga ttatggttgt
     1381 accgcaagcg ccctcgcgta taaaattgcc tcgcttaagc acacggatga gagacagcct
     1441 cctctcctcc gtgtgttact ataaaagtta tctcccttct cgttcatcgt tccatatttg
     1501 agaaacagta tgtcttccag agttttgacc ccggacgtcg ttggtattga cgccctggta
     1561 cacgatcacc aaaccgttct ggcaaaagct gaaggcggtg tggttgccgt atttgctaac
     1621 aatgccccgg cgttttatgc cgtcacgcct gcacgcctgg ctgaactgct ggcgctggaa
     1681 gaaaagctgg cgcgtccggg aagcgatgtc gctctggacg atcaactcta tcaggaaccg
     1741 caagccgctc ccgttgctgt ccccatgggg aaattcgcca tgtatccgga ctggcaaccc
     1801 gatgccgact ttatccgcct ggcggcgcta tggggcgtgg cgctaagaga gccggtgacc
     1861 accgaagaac tggcctcatt cattgcctac tggcaggcgg aaggtaaagt ctttcaccat
     1921 gtgcagtggc aacaaaaact ggcgcgcagc ctgcaaatcg gtcgtgccag caacggcgga
     1981 ctgccgaaac gagatgtgaa tacggtcagc gaacctgaca gccaaattcc accaggattc
     2041 agagggtaac gatgaaaaac gttggcgacc tgatgcaacg cctgcaaaaa atgatgcctg
     2101 cccatatcaa acctgcattc aaaacgggtg aagagctact ggcctggcag aaagaacaag
     2161 gggcgatccg ctccgccgct ctcgaacgtg aaaatcgggc gatgaaaatg cagcgcacct
     2221 ttaaccgctc cggtattcgt ccactgcatc agaactgctc ctttgagaac tatcgcgttg
     2281 agtgtgaagg gcagatgaat gcgttaagca aagcgcgcca gtatgtcgaa gagtttgacg
     2341 gcaacatcgc cagcttcatc ttttctggta agccaggaac cggcaaaaac catctggcgg
     2401 cggcaatctg caacgagctg ctgctacgcg gtaaatccgt attgatcatc accgtggccg
     2461 atattatgtc ggcgatgaaa gataccttca ggaatagcgg taccagcgaa gaacaactgc
     2521 ttaacgatct gagcaacgtt gatctgctgg tgatcgatga gatcggcgtg cagaccgaat
     2581 cgaaatacga aaaagtgatc atcaaccaga tcgtcgatcg ccgctcttct tccaaacgcc
     2641 caaccgggat gctgaccaac agtaatatgg aagagatgac caagttgctg ggcgaacgcg
     2701 tgatggaccg tatgcgcctg ggtaacagtt tgtgggtgat cttcaactgg gatagctacc
     2761 gtagccgggt aacaggtaaa gagtattaat ccccgtttct ggcggtatac tgcgacacaa
     2821 ttccaccttg agtaatgatg atgaaaactg ttaaacatct tctgtgctgt gccattgccg
     2881 ccagcgcatt aatttccacc ggggtgcatg ctgcgtcctg gaaagatgcg ctctccagcg
     2941 ccgccagcga acttggcaac caaaacagca cgacacagga aggcggttgg tcgctcgcgt
     3001 cattaactaa cttgcttagc agcgggaacc aggccttaag cgcagataac atgaacaacg
     3061 ccgcaggcat tctgcaatac tgcgcgaagc aaaagctggc gtcggtaacc gatgccgaaa
     3121 acatcaagaa ccaggtgctg gaaaagctgg gcctgaacag tgaagaacaa aaagaagaca
     3181 ccaactatct ggatggcatt cagggtttgc tgaaaaccaa agatggtcag caactcaatc
     3241 tggataacat cggaacgact ccgctggcag aaaaggtgaa aaccaaagcc tgcgatctgg
     3301 tgttaaagca ggggctgaac ttcatttcct gaaccgcgaa acgccgcgtt ttagctgtta
     3361 cccggctgac gcggctctct cctgcccttt tccctgctcc tggacggttt tccccctgtt
     3421 acacgggaag cgaccgcgat caaaaaagtc tgtttataaa ctaattccta acaacatcgc
     3481 atttttttga cactagaatt gcagcaatat ggcgacgcca ttacattgtg cttctcaaaa
     3541 agcttaaacg ttggctaaac ggcccggttt agtgaggatc gcccgttgtc agaactactc
     3601 tctttcgccc tttttctcgc ctctgtgctg atttacgcat ggaaagcggg acgtaacacc
     3661 tggtggtttg cagccacatt aacggtgctg gggctatttg tcgttttaaa tatcaccctg
     3721 tttgccagcg actattttac tggcgctggt attaacgacg cggttctcta taccttaacc
     3781 aacagcctga ccggtgctgg cgtcagcaaa tacatcctgc cgggtatcgg cattgtgctg
     3841 gggctgacag cggtgttcgg tgcgctgggc tggatcctgc gccgtcgtcg ccatcatccg
     3901 caccattttg gttacagcct gctggcgctc ttactggcgc tgggttcggt ggacgccagc
     3961 ccggcatttc gtcagataac ggaactggtg aaatcccagt cacgcgatgg cgacccggac
     4021 tttgcggctt attataaaga gccgtcgaaa actatccctg acccgaaact caacctggtt
     4081 tatatctacg gcgaaagtct cgagcggacc tattttgata acgaagcttt cccggatctc
     4141 acgcctgaac tgggcgcgtt gaaaaatgaa ggcctggatt tcagccacac gcagcagctg
     4201 ccgggaacag attacacgat tgcgggcatg gtggcttctc agtgcggcat tccgcttttt
     4261 gccccatttg aaggtaacgc ctccgcttct gtttccagct tcttcccgca gaacatctgt
     4321 ctgggcgata tcctgaaaaa ctcgggttat cagaactatt tcgtgcaggg cgcgaatctg
     4381 cgttttgccg gtaaagatgt gttcctgaag tcgcatggct tcgaccactt atacggctca
     4441 gaagagctga aaagcgtggt agccgacccg cactatcgca acgactgggg attctacgac
     4501 gataccgttc tcgatgaagc gtggaaaaag tttgaagagc tttcccgctc aggtcagcga
     4561 ttctcactgt ttaccctgac agtcgatacc catcacccgg atggttttat ctcgcgtacc
     4621 tgtaaccgca aaaaatatga ttttgacggt aagccgaacc agtcattcag cgcggtaagt
     4681 tgcagccagg agaatatcgc ggcgtttatc aacaaaatca aagcgtcacc gtggtttaaa
     4741 gatactgtca tcgtcgtctc ttctgaccat ttagcgatga acaacacggc gtggaaatac
     4801 ctcaataagc aggatcgcaa taacctgttt tttgtcattc gtggcgacaa gccgcagcaa
     4861 gagacgctgg cagtgaagcg taacacgatg gataacggcg cgacggtgct ggacattctc
     4921 ggtggcgata actatctcgg acttggtcgt agcagtttat ccgggcagtc aatgtcggaa
     4981 atcttcctca atatcaaaga gaaaacattg gcgtggaagc cggatatcat ccgcctgtgg
     5041 aaattcccta aagagatgaa agagttcacc atcgaccagc agaaaaacat gattgccttc
     5101 tcgggtagcc atttccgttt gccgctgctg ttgcgggttt cagacaaacg cgtggaaccg
     5161 ctgccggaaa gcgaatactc agcaccgctg cgtttccagc tggccgattt cgctccacgc
     5221 gacaatttcg tctgggttga ccgttgctac aagatggcac aactctgggc tccggaactg
     5281 gcactttcca ccgactggtg tgtctcgcag gggcagcttg gcggtcagca aattgttcag
     5341 catgttgaca aaacaacatg gcagggcaaa acggcattta aagatacggt catcgacatg
     5401 gcgcgttaca aaggcaatgt cgatacgctg aagattgttg ataacgatat tcgctacaaa
     5461 gccgacagtt tcatcttcaa cgtcgccggt gcgccagaag aggtgaaaca gtttagtggg
     5521 atttcccgtc cagagtcgtg gggccgctgg tccaacgcgc agctgggcga tgaagtaaaa
     5581 atcgagtaca agcatccgct gccgaagaaa tttgacctgg tgattaccgc caaagcatac
     5641 ggcaataacg ccagccgtcc tattccggta cgcgtaggca atgaagaaca aacccttgtg
     5701 ctgggcaatg aagtgaccac caccacactg catttcgata acccaaccga tgccgacaca
     5761 ctggtaattg tgccgccgga acctgtctca accaacgaag ggaatatcct cggacactcg
     5821 ccgcgtaagc tcgggatcgg catggtggaa attaaagttg tggaacgtga agggtaattc
     5881 ctgactgatg tgtaggtcgc ataaggtgcg acctacacac cttctccggc ctacaaaaca
     5941 tcattgagaa acagaaaaaa acagcgccca acacttcccc ggacgctgca tggcaacttt
     6001 aataatctcg ccttatcaga aagtaattac acctttaatt aactcacgat tgttaatcac
     6061 atcgcgctcg taggtttctg ccagcgtggc gaacggatag cgatgggtta acatcatgtc
     6121 agcagtgatt tttccttccg ccatcagtcg accgacttta gcaaaatctt ccggcgtggc
     6181 gttgcggctg cccatcatcg tcgtttcttt tttatggaat tccggatcgg agaactgcaa
     6241 ctcaccttta aacaggccga caaataccac cgtgccgccg tgacgaatca gattcacggt
     6301 gttattcatc gcatgttgat tacctgtcgc gtcgatcact ttctgcgcca gcgaaccacc
     6361 aaactgcgcc cgcaactgcg cgtcaaaatc ttcggctgac gggtccagca cgggcaactc
     6421 cagacgcgtt gccacatgtt cacggcgcgc cggactggta tccgccacca ccacctgtgc
     6481 gccatcggct ttagcgattg ccgccgcgcc cagaccgatt ggccccgccc cgaccaccag
     6541 cacctgctcg ccgggagcaa tggctgcgcg acgcaccgca tgagcgctaa tagcgaaagg
     6601 ttcaatcaat gcggccgcct gcgggtcaat accgtctgcg ggcaaaatgt tcgccaccgg
     6661 cacgctcaaa tactcactaa aaccgccatc ctgatgcacg ccaatgactg aaattttttc
     6721 acagcaattg gtacgcccgc ttttacacgc cgggcattgc tgacaagaaa tatacggaat
     6781 taccgcaact tgctgtccga tattaaaatt gtgaaagttt tttcctgatt caataatctc
     6841 cccacatatt tcatggccta aaacgcgtgg atagctaaaa aatggttgat tgcccgccca
     6901 ggcatgaata tctgtaccgc aaatcccgac ggttttaatt ttaattaatg cttcattatc
     6961 gcctggcacc ggtctttccc ggactgtcca tactaaattc tttatttcct gacaaattaa
     7021 tactttcatt gtggacatga ttcaccgctc cttttatttt atgactttca gatatcgtcg
     7081 aatttataag agcacctgaa tttacccata aaatatgcgg atgatcacgc ataaaaatgt
     7141 tttaaatcgg tttttaatgc cggactggag ggaagaatga gtcgttcgca gaatttacgt
     7201 cataacgtca taaatcaggt gattgaagat atggcgaggg gcaatattcc ttctccgctg
     7261 ccttcacaaa gtgggctggc agaaatgtat aacatcagtc gcactacggt acgccacata
     7321 ctccagcatt taagcgcatg tggcgtgctc accctggtgg ggaaaaatta tgttatcgcc
     7381 cgcaaacccg aacagaacga tggatttgat tgcatcacgg cctcgctgaa agagcaaaac
     7441 cgtcttttcg agcaggcatt tttcaccatg attaacctgc gccagttacg cgcgggagaa
     7501 agcttttccg aacttcagct ggcgcgagcg gcaggcgtca gtccggtagt agtacgagaa
     7561 tatcttttga aattcggtcg ttataatctt attcaaagcg aaaagcgcgg ccagtggagc
     7621 atgaagcagt tcgatcaatc ctacgccgag cagctgtttg agctacgcga aatgctcgaa
     7681 acacattccc tgcaacactt tctcaacctt cccgatgacg atccccgctg gttgcaggcg
     7741 aaaaccatgc tcgaacgtca caggacattg cgcgacagca ttggcagcaa ctttcggatg
     7801 ttttctcaat tagaccgtga ttttcatgcc atgctgttat ccgctgctga caacatcttt
     7861 tttaatcaat cacttgaaat tatctcggtg atctttcatt ttcactatca atgggatgaa
     7921 agcgatctca agcagcgcaa tatcattgct gttgatgagc atatgaccat tctcagcgcc
     7981 ctgatttgcc gtagcgattt agatgcaata acggcattgc gaaaccatct ggataccgcc
     8041 aaacaatcaa tgattcgttc catcaggcaa catcaccgat aaattaagta cagattaaaa
     8101 acattgattc ttggtttata tcactatgtt gtattaatgc agtattaatc caggcgattg
     8161 ttctgcccat aaggaaatac atagggaata aacgatgctg gaagtattac tctcagccat
     8221 catttaactc cctattactg gtagcgataa aattatcggc tatccatgat taacgtttcc
     8281 agcacatcta attcactgac tatttttgcc agtgtttcat cactgacttc ttgccgtgca
     8341 cgcatctggt aaatagccgc acgcctggca tttaacgctg ccagattaaa tgttctttcc
     8401 agcttcgcgc gctgcatatg taaagaatta tcattttccg acatatcatc ttcgctacgc
     8461 cgcatataac cgataactct ggcccctgtt tccataatca actcgttgtc gaggttttct
     8521 gtactggtgc gtagcaaacg ttgctcagtc ttttgcacac tgagtatggc cattttggcc
     8581 ataaacagat cggcagcttc ctcttctttt tgccggttat cgctgaaaat gcccacattt
     8641 ttccccagta gtaacggcag aataataatg ccgcaaacca gcgaaaagag gatcacacct
     8701 gtggcgagaa ataccagtaa gtaacgcccg ggaaaaggtg tgccatccgg cagcaataac
     8761 gggatagaaa gcacccccgc aagcgtgatg gctccgcgca cccccgcaaa ggtcgccagc
     8821 agcatatgac gcgttttata tccagcaaac agcatcggat gtttgccggg tcgtttacgg
     8881 ctatacagac gcatcagcca aagccagccc aggcgcacaa ccattaacgc caggtagatc
     8941 caaatgacat caaggagcag cgatccggtg tctaccagtg gatcgttttc tgcacggatc
     9001 agtgattccg aaataatgcc cggcagttgt aagcctaaaa tcagaaacac catgccgtta
     9061 aagacatatt caagcatcga ccagctactt ttagctttaa ttctcaggcg gataggcgcg
     9121 tggtggacta cgccaatgcg actggttgtt aaccccgcgg cgacagcggc aagaatgcca
     9181 gataagccaa aatgttccgc aaccagataa acaataaacg gcaacagaag catcaataca
     9241 atctgaatgg aaggttcatc gtgagcatat cgactgatca acagcagccc tttgccatat
     9301 aaccaggtaa cagcaatccc cacggcaata ccgcccaacg caacgacaaa aaacgcaatg
     9361 gttgcgccat gaacgctgaa ttccatcgtt cccatcgtca ctgcaacagc aaatttcaac
     9421 gccaccaggc cggaagcatc attcatcaac gcttcgcctt cgagcacttc ctggatgttt
     9481 ttgctgatac gccctttccc gacaatcccc agcaacgcca cggcatcggt aggcgacaac
     9541 acggcggcta atgcgaaagc agcgatgagc ggcacattcg gtaagagtaa atgaatcaca
     9601 tagccgacac caaaaatagt gattagcacc aacacaagcg ccaggctgat aatttcccga
     9661 ctgtgcttga tcaattcttt aggttgtatt tttgagcttt cagcaaacaa caaggggggt
     9721 ataaacaata agagaaaaag ttcaggattg aattccacat gcagaccaaa tatgggctgg
     9781 gccagtacag cgccagcggc aatctggatc aaaggaagtg gtatctgaac aggagacaga
     9841 cgcgtaagc
//
LOCUS       DAZKHF020000172         9739 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2201S31241
            SAMN43267800-rid25106413.denovo.173, whole genome shotgun sequence.
ACCESSION   DAZKHF020000172 DAZKHF020000000
VERSION     DAZKHF020000172.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267800
            Sequence Read Archive: SRR30307263
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 9739)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9739)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 9739)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350581.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350581.2
            Genome Coverage       :: 151x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:21:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,652
            CDSs (total)                      :: 5,557
            Genes (coding)                    :: 5,320
            CDSs (with protein)               :: 5,320
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 237
            CDSs (without protein)            :: 237
            Pseudo Genes (ambiguous residues) :: 0 of 237
            Pseudo Genes (frameshifted)       :: 102 of 237
            Pseudo Genes (incomplete)         :: 130 of 237
            Pseudo Genes (internal stop)      :: 52 of 237
            Pseudo Genes (multiple problems)  :: 41 of 237
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9739
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267800-rid25106413.denovo.173"
                     /serotype="O 26:H11"
                     /isolate="2201S31241"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-01"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            279..1592
                     /gene="gltP"
                     /locus_tag="ACHOSC_RS16895"
                     /old_locus_tag="pgaptmp_003379"
     CDS             279..1592
                     /gene="gltP"
                     /locus_tag="ACHOSC_RS16895"
                     /old_locus_tag="pgaptmp_003379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015293 - symporter activity [Evidence
                     IEA];
                     GO_process: GO:0006835 - dicarboxylic acid transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate/aspartate:proton symporter GltP"
                     /protein_id="HGU4549266.1"
                     /translation="MKSIKFSLAWQILFAMVLGILLGSYLHYHSDSRDWLVVNLLSPA
                     GDIFIHLIKMIVVPIVISTLVVGIAGVGDAKQLGRIGAKTIIYFEVITTVAIILGITL
                     ANVFQPGAGVDMSQLATVDISKYQSTTEAVQSSSHGIMGTILSLVPTNIVASMAKGEM
                     LPIIFFSVLFGLGLSSLPATHREPLVTVFRSISETMFKVTHMVMRYAPVGVFALIAVT
                     VANFGFSSLWPLAKLVLLVHFAILFFALVVLGIVARLCGLSVWILIRILKDELILAYS
                     TASSESVLPRIIEKMEAYGAPASITSFVVPTGYSFNLDGSTLYQSIAAIFIAQLYGID
                     LSIWQEIILVLTLMVTSKGIAGVPGVSFVVLLATLGSVGIPLEGLAFIAGVDRILDMA
                     RTALNVVGNALAVLVIAKWEHKFDRKKALAYEREVLGKFDKTADQ"
     gene            complement(1670..2359)
                     /gene="yjcO"
                     /locus_tag="ACHOSC_RS16900"
                     /old_locus_tag="pgaptmp_003380"
     CDS             complement(1670..2359)
                     /gene="yjcO"
                     /locus_tag="ACHOSC_RS16900"
                     /old_locus_tag="pgaptmp_003380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Sel1 family TPR-like repeat protein YjcO"
                     /protein_id="HGU4549267.1"
                     /translation="MKKIIALMLFLTFFAHANDSEPGSQYLKAAEAGDRRAQYFLADS
                     WFSSGDLSKAEYWAQKAADSGDADACALLAQIKITNPVSLDYPQAKVLAEKAAQAGSK
                     EGEVTLAHILVNTQAGKPDYPKAISLLENASEDLENDSAVDAQMLLGLIYANGVGIKA
                     DDDKATWYFKRSSAISRTGYSEYWAGMMFLNGEEGFIEKNKQKALHWLNLSCMEGFDT
                     GCEEFEKLTNG"
     gene            complement(2453..4600)
                     /gene="fdhF"
                     /locus_tag="ACHOSC_RS16905"
                     /old_locus_tag="pgaptmp_003381"
     CDS             complement(2453..4600)
                     /gene="fdhF"
                     /locus_tag="ACHOSC_RS16905"
                     /old_locus_tag="pgaptmp_003381"
                     /EC_number="1.17.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009326 - formate dehydrogenase complex
                     [Evidence IEA];
                     GO_function: GO:0008863 - formate dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_process: GO:0015942 - formate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_except=(pos:complement(4181..4183),aa:Sec)
                     /transl_table=11
                     /product="formate dehydrogenase subunit alpha"
                     /protein_id="HGU4549268.1"
                     /translation="MKKVVTVCPYCASGCKINLVVDNGKIVRAEAAQGKTNQGTLCLK
                     GYYGWDFINDTQILTPRLKTPMIRRQRGGKLEPVSWDEALNYVAERLSAIKEKYGPDA
                     IQTTGSSRGTGNETNYVMQKFARAVIGTNNVDCCARVUHGPSVAGLHQSVGNGAMSNA
                     INEIDNTDLVFVFGYNPADSHPIVANHVINAKRNGAKIIVCDPRKIETARIADMHIAL
                     KNGSNIALLNAMGHVIIEENLYDKAFVASRTEGFEEYRKIVEGYTPESVEDITGVSAS
                     EIRQAARMYAQAKSAAILWGMGVTQFYQGVETVRSLTSLAMLTGNLGKPHAGVNPVRG
                     QNNVQGACDMGALPDTYPGYQYVKDPANREKFAKAWGVESLPAHTGYRISELPHRAAH
                     GEVRAAYIMGEDPLQTDAELSAVRKAFEDLELVIVQDIFMTKTASAADVILPSTSWGE
                     HEGVFTAADRGFQRFFKAVEPKWDLKTDWQIISEIATRMGYPMHYNNTQEIWDELRHL
                     CPDFYGATYEKMGELGFIQWPCRDTSDADQGTSYLFKEKFDTPNGLAQFFTCDWVAPI
                     DKLTDEYPMVLSTVREVGHYSCRSMTGNCAALAALADEPGYAQINTEDAKRLGIEDEA
                     LVWVHSRKGKIITRAQVSDRPNKGAIYMTYQWWIGACNELVTENLSPITKTPEYKYCA
                     VRVEPIADQRAAEQYVIDEYNKLKTRLREAALA"
     gene            complement(4798..6264)
                     /gene="mdtP"
                     /locus_tag="ACHOSC_RS16910"
                     /old_locus_tag="pgaptmp_003382"
     CDS             complement(4798..6264)
                     /gene="mdtP"
                     /locus_tag="ACHOSC_RS16910"
                     /old_locus_tag="pgaptmp_003382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux transporter outer membrane
                     subunit MdtP"
                     /protein_id="HGU4549269.1"
                     /translation="MINRQLSRLLLCSILGSTTLISGCALVRKDSAPHQQLKPEQIKL
                     ADDIHLASSGWPQAQWWKQLNDPQLDALLQRTLSGSHTLAEAKLREEKAQSQADLLDA
                     GSQLQVAALGMLNRQRVSANGFLSPYAMDAPALGMDGPYYTEATVALFAGLDLDLWGV
                     HRSAVAAAIGAHNAALAETAAVELSLTTGVAQLYYSMQASYQMLDLLEQTRDVIDYAV
                     KAHQSKVAHGLEAQVPFQGARAQILAVDKQIAAVKGQITETRESLRALIGAGASDMPE
                     IKPVALPRVQTGIPATLSYELLARRPDLQAMRWYVQASLDQVDSARALFYPSFDIKAF
                     FGLDAIHLDTLFKKTSRQFNFIPGLKLPLFDGGRLNANLEGTRAASNMMIERYNQSVL
                     NAVRDVAVNGTRLQTLNDEREMQAERVEATRFTQRAAEAAYQRGLTSRLQATEARLPV
                     LAEEMSLLMLDSRRVIQSIQLMKSLGGGYQAAPVVEKK"
     gene            complement(6261..8312)
                     /gene="mdtO"
                     /locus_tag="ACHOSC_RS16915"
                     /old_locus_tag="pgaptmp_003383"
     CDS             complement(6261..8312)
                     /gene="mdtO"
                     /locus_tag="ACHOSC_RS16915"
                     /old_locus_tag="pgaptmp_003383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418505.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux transporter permease subunit
                     MdtO"
                     /protein_id="HGU4549270.1"
                     /translation="MSALNSLPLPVVRLLAFFHEELSERRPGRVPQTVQLWVGCLLVI
                     LISMTFEIPFVALSLAVLFYGIQSNAFYTKFVAILFVVATMLEIGSLFLIYKWSYGEP
                     LIRLFIAGPILMGCMFLMRTHRLGLVFFAVAIVAIYGQTFPAMLDYPEVVVRLTLWCI
                     VVGLYPTLLMTLIGVLWFPSRAISQMHQALNDRLDDAISHLTDSLAPLPETRIEREAL
                     ALQKLNVFCLADDANWRTQSAWWQSCVATVTYIYSTLNRYDPTSFADSQAIIEFRQKL
                     ASEINKLQHAVAEGQCWQSDWRISESEAMAARECNLENICQTLLQLGQMDPNTPPTPA
                     AKPPSMAADAFTNPDYMRYAVKTLLACLICYTFYSGVDWEGIHTCMLTCVIVANPNVG
                     SSYQKMVLRFGGAFCGAILALLFTLLVMPWLDNIVELLFVLAPIFLLGAWIATSSERS
                     SYIGTQMVVTFALATLENVFGPVYDLVEIRDRALGIIIGTVVSAVIYTFVWPESEART
                     LPQKLAGTLGMLSKVMRIPRQQEVTALRTYLQIRIGLHAAFNACEEMCQRVALERQLD
                     SEERALLIERSQTVIRQGRDILHAWDATWNSAQALDNALQPDRAAQFADALEKYAAGL
                     ATALSRSPQITLEETPASQAILPTLLKQEQHVCQLFARLPDWTAPALTPATEQAQGAT
                     Q"
     gene            complement(8312..9343)
                     /gene="mdtN"
                     /locus_tag="ACHOSC_RS16920"
                     /old_locus_tag="pgaptmp_003384"
     CDS             complement(8312..9343)
                     /gene="mdtN"
                     /locus_tag="ACHOSC_RS16920"
                     /old_locus_tag="pgaptmp_003384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux transporter periplasmic adaptor
                     subunit MdtN"
                     /protein_id="HGU4549271.1"
                     /translation="MESTPKKAPRSKFPALLVVALALVALVFVIWRVDSAPSTNDAYA
                     SADTIDVVPEVSGRIVELAVTDNQAVKQGDLLFRIDPRPYEANLAKAEASLAALDKQI
                     MLTQRSVDAQQFGADSVNATVEKARAAAKQATDTLRRTEPLLKEGFVSAEDVDRARTA
                     QRAAEADLNAVLLQAQSAASAVSGVDALVAQRAAVEADIALTKLHLEMATVRAPFDGR
                     VISLKTSVGQFASAMRPIFTLIDTRHWYVIANFRETDLKNIRSGTPATIRLMSDSGKT
                     FEGKVDSIGYGVLPDDGGLVLGGLPKVSRSINWVRVAQRFPVKIMVDKPDPEMFRIGA
                     SAVANLEPQ"
     gene            complement(9362..9637)
                     /locus_tag="ACHOSC_RS16925"
                     /old_locus_tag="pgaptmp_003385"
     CDS             complement(9362..9637)
                     /locus_tag="ACHOSC_RS16925"
                     /old_locus_tag="pgaptmp_003385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtcA family lipoprotein"
                     /protein_id="HGU4549272.1"
                     /translation="MPTVLSRMAMQLKKTAWIIPVFMVSGCSLSPAIPVIGAYYPSWF
                     FCAIASLILMLITRRVIQRANINLAFVGIIYTALFALYAMLFWLAFF"
BASE COUNT         2206 a         2592 c         2603 g         2338 t
ORIGIN      
        1 aaaataatca ccaatcagta ttattgcaga ttaaacaaat aaaaatcttt ccataacaaa
       61 tggttattca ttaatcctgc cataaactcg cgttattatg cattaatgca gcgaaaagct
      121 ctgttgttaa agggttgcgc aacataccgc gcaaatgata ctgatcataa gcgttaaaaa
      181 aatctacaaa ccaacgcaac acaattcatg ccctggcagt atgtcacgtt ctcgcgtttc
      241 tgaacgggga acggcgctcc attgaggaag ttattctgat gaaaagtata aaattcagcc
      301 tggcctggca gattctgttt gctatggtgc tgggcattct cctgggaagc tacctgcact
      361 accatagcga cagccgcgac tggctggtcg tcaatttgct ctctccggcg ggtgatatct
      421 tcatccatct gatcaaaatg attgttgtgc cgattgtgat ctccacgctg gtggtgggta
      481 tcgcgggtgt tggtgatgcc aaacagctcg ggcgtattgg cgcgaaaacc attatttact
      541 tcgaggtgat caccaccgtc gccatcattt tggggatcac tctggcgaac gtcttccagc
      601 ccggtgccgg ggtggatatg tcgcagctgg cgaccgtcga tatctcgaaa tatcagagca
      661 ctacggaagc ggtacaaagc agttcccacg gcattatggg cacgattttg tcgctggtgc
      721 cgacgaacat tgtggcgtcg atggcgaaag gcgaaatgct gccgatcatc ttcttctcgg
      781 tgctgtttgg tctggggctt tcctccctgc ccgcgacgca tcgtgaaccg ctggtgaccg
      841 tgttccgctc catctctgaa accatgttta aagtgactca catggtgatg cgttatgcac
      901 cggtgggtgt gtttgcgctg attgcggtga cggtggctaa ctttggtttc tcttctctgt
      961 ggccactggc gaaactggtg ctgctggtgc atttcgccat tctgttcttc gcgctggtag
     1021 tgctgggaat tgtggcgcgc ctgtgcgggt taagcgtctg gatcctgatt cgtattctga
     1081 aagatgagct gattctggcg tactccactg ccagctctga aagcgtactg ccgcgaatta
     1141 ttgagaagat ggaagcctac ggagcaccgg cgtcgatcac cagcttcgtg gtgccgaccg
     1201 gttactcttt taaccttgat ggttcgacgc tgtatcaaag tattgccgct attttcatcg
     1261 ctcagctgta tggcattgac ctgtccatct ggcaggaaat cattctggtg ctgacgctga
     1321 tggtgacctc gaaagggatt gctggcgtgc cgggcgtgtc gtttgtggtg ctgctggcaa
     1381 cgctgggtag cgtaggtatt ccgctggaag gcctggcgtt tattgctggt gttgaccgta
     1441 tcctcgacat ggcgcgtact gcgctgaacg tggtgggtaa tgcgctggcg gtgctggtga
     1501 ttgccaagtg ggaacacaaa tttgaccgca aaaaggcgct ggcttatgag cgtgaagtgc
     1561 tgggcaaatt tgataaaact gcggatcaat aattgaagat tgccggggtt atccacccgg
     1621 caatgtgtgg atgcctgatg cgacgctggt gcgtcttatc aggcctacct taaccgttgg
     1681 ttaatttttc aaactcttca cacccggtat caaacccttc catacagctc aggttcaacc
     1741 agtgcagcgc cttttgtttg ttcttctcga taaaaccttc ctcaccgttt aagaacatca
     1801 ttcccgccca gtactcggaa taaccggttc gggaaattgc agagctgcgt ttgaaatacc
     1861 aggttgcctt gtcatcgtcg gccttaatgc ccacgccgtt ggcgtaaatc aaaccaagca
     1921 gcatttgggc atcgacggcg gagtcattct ccagatcttc cgaggcgttt tctaacagcg
     1981 aaatcgcctt tggataatcc ggtttacccg cttgagtatt taccagaata tgcgccagcg
     2041 ttacttcacc ttctttactg cccgcttgcg ccgctttctc tgcaagaact tttgcttgtg
     2101 gatagtccag actgaccgga ttggtgattt tgatctgcgc cagcagcgcg caggcatcag
     2161 catcaccgct gtcggcggct ttctgtgccc aatattcggc tttgctcaaa tcgccggagc
     2221 taaaccagct gtcggcaaga aaatattgtg cgcgtcggtc cccggcctct gctgccttta
     2281 aatactggct gccaggctcg gagtcgttcg cgtgggcaaa gaatgtcaaa aacaacatca
     2341 atgcgataat ttttttcatt ttgataatac gattgggtag ggagtaacca gtataaagag
     2401 ttcgaatgga taaaaaaaca gcctccgaaa ggaggctgta gaaaggactg tattacgcca
     2461 gtgccgcttc gcgcaggcga gttttcaact tgttgtactc gtcaatcacg tactgctcgg
     2521 cggcgcgctg atcggcgatc ggctcgacgc gcacggcgca gtatttgtat tccggcgttt
     2581 tcgtaatcgg gcttaagttt tcggtaacca gctcgttaca ggcaccaatc caccactggt
     2641 aggtcatgta aatcgcccct ttgttcggac gatcgctgac ctgcgcacgg gtgataattt
     2701 tgcctttacg cgagtgcacc caaaccaatg cctcatcttc aatacccaga cgtttggcgt
     2761 cttcggtatt gatttgtgcg tagccaggtt catcagccag cgccgccagt gccgcacagt
     2821 taccggtcat cgaacggcaa gagtagtgac caacttcacg caccgttgac agtaccatcg
     2881 ggtactcgtc ggtgagtttg tcgattggcg ctacccagtc gcaggtgaag aactgtgcca
     2941 gaccgttcgg ggtatcaaac ttctctttaa acagataaga agtcccctga tcggcatctg
     3001 aagtatcgcg gcaaggccac tgaatgaagc ccagttcgcc cattttctcg taagtcgcac
     3061 cgtagaaatc cgggcacaga tgacgcaact catcccagat ctcctgggtg ttgttgtagt
     3121 gcatcggata acccatacgg gtggcgattt cactgatgat ttgccagtcc gttttcagat
     3181 cccatttcgg ttcaactgct ttgaagaaac gctggaagcc acggtcagcc gcagtaaaca
     3241 cgccttcatg ctcgccccac gacgttgacg gcaaaatgac atccgccgcc gacgcggttt
     3301 tggtcataaa gatgtcctga acgataacca gttccagatc ttcaaaggct ttacgtactg
     3361 ccgacagctc cgcgtcagtt tgcagcggat cttcgcccat aatgtacgcg gcacgcactt
     3421 cgccatgcgc tgcgcggtgc ggcagctcgc tgatgcgata accggtatga gcaggcaggc
     3481 tttccacgcc ccaggctttg gcgaatttct cgcggttagc cggatctttc acgtactggt
     3541 atcccggata cgtatccggc agcgcgccca tatcgcaggc accctgcacg ttgttctgac
     3601 cacgaaccgg gtttacaccc gcatgcggct taccgaggtt accggtcagc atcgcgaggc
     3661 tggtcagaga acgcacggtt tccacgccct ggtagaactg ggttacaccc atgccccaca
     3721 ggatggcggc gcttttcgcc tgggcataca tccgtgccgc ctgacgaatc tcactggcgc
     3781 tgacgccggt gatatcttca accgactccg gcgtgtagcc ttcaacgatt ttacgatact
     3841 cttcaaagcc ttctgtacgg gaagcgacga acgctttgtc gtacagattt tcttcaataa
     3901 tgacatggcc catcgcattc aacagcgcga tgttcgagcc gtttttcagt gcaatgtgca
     3961 tgtcagcaat gcgcgcggtt tcaattttgc gcggatcgca gacgataatt ttcgccccgt
     4021 tacgtttagc gttaattacg tgattcgcca cgattgggtg ggaatccgcc gggttgtacc
     4081 cgaaaacgaa cactaaatcg gtattatcaa tttcgttaat agcattgctc attgcgccat
     4141 taccgaccga ttggtgcaga cctgcaaccg atgggccgtg tcagacacga gcgcagcagt
     4201 caacgttatt ggtaccaata acggcgcgcg caaatttttg cattacatag ttggtttcgt
     4261 tacccgtacc acgcgaggag ccggtcgtct ggatggcatc cggaccgtac ttctctttga
     4321 tggcgctcag gcgctcggca acgtaattca gtgcctcatc ccaggaaaca ggttcgagtt
     4381 tgccgccacg ctggcgacgg atcatggggg ttttcaggcg cggggtcagg atctgggtat
     4441 cgttaatgaa atcccagcca taataaccct tcagacacag ggtaccctgg ttggttttcc
     4501 cctgcgctgc ctccgcccgg acgattttgc cgttatcgac gaccaggttg attttgcaac
     4561 ctgatgcgca ataggggcaa accgtgacga cttttttcat cggtctcgct ccagttaatc
     4621 aaatcacgca tacgcgctct cgactacagt atgcatcttt tatgccacat tttatttggg
     4681 gtcattccct gatattacgg gcactattta ttcaaaactc tgacgaaaaa caggctgtcg
     4741 tcagttttga cgtgacgaaa cgaaataccg cgtgacagcc atcacgcggc agacatttta
     4801 ttttttctcg acgacgggag ccgcctgata cccgccgccc agcgatttca tcaactgaat
     4861 gctttggatc acccggcggc tgtccagcat cagtaatgac atctcttcgg caagtactgg
     4921 caaccgggct tcagtggcct gtaagcggct ggttaagccg cgctgatagg cggcctcggc
     4981 agcgcgctgg gtgaagcgcg ttgcttccac gcgttcagcc tgcatttctc gctcgtcgtt
     5041 gagcgtttgc agacgcgtgc cgttgacggc aacgtcacgc accgcgttca gtactgactg
     5101 gttgtaacgt tcaatcatca tgttgctggc ggcgcgcgtg ccttcgagat tggcattcaa
     5161 ccgtccaccg tcaaacagcg gcaatttcag acccgggatg aagttgaact ggcgactggt
     5221 ttttttgaat aaggtatcca ggtggatggc atcaaggccg aaaaacgctt tgatatcaaa
     5281 gctcgggtag aacagcgccc gcgcggaatc cacctgatct aatgacgcct gaacatacca
     5341 gcgcatggct tgcagatccg ggcgtctggc gagcaactca taagagagtg tcgccggaat
     5401 gccggtctgg actcgtggta atgccaccgg tttgatctcc ggcatatcgc tggcccccgc
     5461 gccaatcaat gcacgcagag attctcgcgt ttcggtgatt tgccctttga cggcagcaat
     5521 ttgtttatcg accgccagaa tctgtgcccg cgcgccctgg aaaggcactt gtgcttccag
     5581 accgtgcgcc actttactct ggtgcgcttt caccgcgtaa tcaatcacat cgcgagtttg
     5641 ttctaacaga tcgagcatct gatagctggc ctgcatactg taataaagct gcgctacgcc
     5701 cgtggtcagc gatagctcta ctgctgcggt ttctgccagc gcggcattat gcgcgccaat
     5761 ggcggcggca accgctgagc gatgcacacc ccacaaatca agatccagtc cggcaaacaa
     5821 agctactgtg gcttccgtat agtacggccc gtccataccc agtgcgggcg catccatcgc
     5881 ataagggctt aaaaagccgt tcgccgagac gcgttggcgg ttgagcatcc ctaacgctgc
     5941 gacctgtaat tgtgaaccgg catctaacaa atcggcctgg gactgcgctt tttcttcccg
     6001 cagtttcgct tcggcgaggg tgtgtgaacc acttagcgtc cgttggagca gcgcatccag
     6061 ctgcgggtca ttgagttgtt tccaccactg cgcctgcggc cagccggagc tggcaagatg
     6121 aatatcgtcg gccagtttga tttgttccgg tttgagctgt tgatgaggtg cagaatcctt
     6181 acgtaccagg gcacagccgg aaatcagcgt cgtgctgccg agaatgctgc acaacagcag
     6241 acgtgaaagt tgacgattga tcattgcgtg gctccttgtg cctgttccgt ggcgggcgtt
     6301 aatgccgggg ctgtccagtc tggcaagcgg gcgaaaagct ggcagacgtg ttgctcctgt
     6361 tttaataagg tgggcaggat ggcctgcgag gcgggtgtct cttcaagcgt tatttgagga
     6421 gaacggctga gtgcggttgc cagaccggca gcgtatttct ccagggcgtc ggcaaactga
     6481 gctgctctgt ccggctgtag tgcgttatcc agcgcctgcg ccgagttcca ggtggcatcc
     6541 caggcgtgaa gaatatcgcg gccctgacga ataaccgttt gcgaacgttc aatcagtaag
     6601 gcgcgttctt cgctgtccag ttgacgctcc agcgccacgc gttggcacat ctcttcacag
     6661 gcattaaacg ccgcatgcag accgatacga atttgcagat aagtgcgcag agccgtgact
     6721 tcctgctggc gtgggatccg cattacttta cttaacatac ccagcgtgcc agccagtttt
     6781 tgcggcagtg tgcgcgcttc actttcaggc cagacaaagg tgtaaatcac cgcggacacc
     6841 acggtaccaa tgatgatacc cagggcgcga tcgcgaattt ccaccaggtc gtacactggg
     6901 ccaaaaacgt tttcgagcgt ggcgagcgcg aaggtgacca ccatctgtgt gccgatataa
     6961 gaagagcgtt cagagctggt ggcaatccat gcgcccaaca ggaaaatcgg tgccagcaca
     7021 aacagcaatt cgacaatatt gtccagccag ggcatgacca gtagcgtgaa taacagcgcc
     7081 agaatcgcgc cgcaaaaggc cccgccaaaa cgcagcacca tcttctggta cgacgaaccg
     7141 acatttgggt tagcgacgat cacgcatgtc agcatacagg tgtgaatgcc ttcccagtcc
     7201 acgccgctgt aaaaggtgta acagatcaaa caggcgagca gcgtttttac cgcgtagcgc
     7261 atatagtctg gattggtaaa agcatcggcg gccattgatg gcggtttggc tgcgggcgtt
     7321 ggcggcgtat tcgggtccat ctgacccagt tgtaacaacg tctggcagat attctccagg
     7381 ttacattccc gtgccgccat cgcttcactt tcactgatcc gccagtcgct ttgccagcac
     7441 tgaccttcag caacggcatg ctgcagctta ttgatttctg aggctaattt ttgccgaaat
     7501 tcaataattg cctgagaatc agcaaaagag gtgggatcgt agcgattcag cgtcgagtaa
     7561 atgtaggtta ccgttgccac gcagctttgc caccatgcgc tttgagttcg ccagttggca
     7621 tcgtccgcga gacaaaagac attcagtttt tgcagcgcca gcgcctctct ttcaatccgc
     7681 gtttcgggta gcggtgcgag gctgtccgtc aggtggctaa tggcatcatc aagccgatca
     7741 ttaagcgcct gatgcatttg cgaaatggca cgactgggaa accacagcac gccgattaac
     7801 gtcatcagca aggttggata aaggccaaca acgatacacc acagcgttaa gcgcacgacc
     7861 acttccggat agtcgagcat ggcggggaag gtttgcccgt aaatggcgac aatggcgact
     7921 gcgaaaaaga ccagtcccag ccgatgggtg cgcatcaaaa acatacagcc catcaggatc
     7981 ggcccggcga tgaacaatcg gatcaacggt tcgccgtatg accatttgta gatcaaaaac
     8041 aggctgccga tctccagcat cgtggcgacc acaaataaga tcgcgacaaa tttggtgtaa
     8101 aacgcgttcg actgaatacc gtaaaacagc actgccagcg ataacgccac gaaagggatc
     8161 tcaaaggtca tcgagatcag aatcaccagc aggcagccta cccagagttg cacggtctgc
     8221 ggcacgcggc ctggtcgccg ctcgcttaac tcttcatgaa agaacgccag cagcctgacc
     8281 accggtaatg gcagggagtt gagcgcgctc attattgcgg ctcaagatta gcgactgccg
     8341 aagcgccgat gcggaacatt tccgggtcag gtttatcgac catgattttg accggaaaac
     8401 gctgggcaac gcggacccag ttaatagagc gagaaacttt cggcaggccg cccagcacca
     8461 ggccgccgtc atccggtagc acgccgtagc caatcgaatc cactttaccc tcgaaggttt
     8521 tgccgctgtc actcatcagg cgaatcgttg cgggtgtacc tgagcgaata tttttcagat
     8581 cggtttcgcg gaagttggcg atcacgtacc agtgacgagt gtcgattagg gtaaaaatag
     8641 ggcgcatggc agaagcaaat tgcccgacgg aggttttgag ggaaatgacc cggccatcaa
     8701 acggcgcgcg aacggtcgcc atttccagat gcagtttggt cagggcaata tccgcttcga
     8761 ccgccgcacg ctgggcaact aatgcatcca cgccgctgac ggcgctggcg gctgactgcg
     8821 cctgcaacaa tacagcatta agatcggctt ctgcagcgcg ctgcgcggtc ctcgcacggt
     8881 caacgtcttc cgctgagaca aaaccttctt tcagtaatgg ctcggtgcgg cgtaatgtat
     8941 ctgtggcctg tttcgcggcg gcacgggctt tttctaccgt ggcgttaacc gagtcggcac
     9001 caaactgttg cgcgtcaacg ctacgctggg tgagcataat ttgcttatcc agcgccgcga
     9061 gggaggcttc agctttcgcc agattggctt cgtacgggcg cgggtcaatg cggaacagca
     9121 aatcgccctg tttgactgcc tggttgtcgg tgaccgccag ttctacaatg cgaccgctga
     9181 cttccggcac cacatcaatg gtatctgctg acgcgtaagc gtcattagtt gatggcgcac
     9241 tgtctacgcg ccagataacg aaaacaaggg caaccagcgc caacgcaacc actaacagag
     9301 cagggaattt actgcgagga gcttttttcg gcgtactttc catgagcgcg tctcaaatag
     9361 attagaaaaa tgccagccag aacagcatgg cgtagagagc aaaaagggcg gtataaataa
     9421 ttccgacaaa tgccagattg atatttgccc gctgaataac tcgcctcgtg atgagcatca
     9481 aaataaggct ggcaatggcg cagaaaaacc agctgggata ataagcaccg atcaccggga
     9541 ttgccggaga taatgagcat cccgaaacca tgaagacggg aattatccag gctgtttttt
     9601 tgagttgcat cgccatgcga gagagaactg ttggcatatt aaatacatcc tgtctgctgc
     9661 gaacaatatc tgtagtgaat atcaatatcc attgaaggcg tcagtatggc tattttctcg
     9721 atgaataaaa atagcttta
//
LOCUS       DAZKHF020000173         9656 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2201S31241
            SAMN43267800-rid25106413.denovo.174, whole genome shotgun sequence.
ACCESSION   DAZKHF020000173 DAZKHF020000000
VERSION     DAZKHF020000173.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267800
            Sequence Read Archive: SRR30307263
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 9656)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9656)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 9656)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350581.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350581.2
            Genome Coverage       :: 151x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:21:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,652
            CDSs (total)                      :: 5,557
            Genes (coding)                    :: 5,320
            CDSs (with protein)               :: 5,320
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 237
            CDSs (without protein)            :: 237
            Pseudo Genes (ambiguous residues) :: 0 of 237
            Pseudo Genes (frameshifted)       :: 102 of 237
            Pseudo Genes (incomplete)         :: 130 of 237
            Pseudo Genes (internal stop)      :: 52 of 237
            Pseudo Genes (multiple problems)  :: 41 of 237
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9656
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267800-rid25106413.denovo.174"
                     /serotype="O 26:H11"
                     /isolate="2201S31241"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-01"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            complement(287..1357)
                     /gene="dadX"
                     /locus_tag="ACHOSC_RS16930"
                     /old_locus_tag="pgaptmp_003386"
     CDS             complement(287..1357)
                     /gene="dadX"
                     /locus_tag="ACHOSC_RS16930"
                     /old_locus_tag="pgaptmp_003386"
                     /EC_number="5.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309712.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008784 - alanine racemase activity
                     [Evidence IEA];
                     GO_process: GO:0006522 - alanine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="catabolic alanine racemase DadX"
                     /protein_id="HGU4549273.1"
                     /translation="MTRPIQASLDLQALKQNLSIVRQAAPHARVWSVVKANAYGHGIE
                     RIWSALGATDGFALLNLEEAITLRERGWKGPILMLEGFFHAQDLEIYDQHRLTTCVHS
                     NWQLKALQNARLKAPLDIYLKVNSGMNRLGFQPDRVLTVWQQLRAMANVGEMTLMSHF
                     AEAEHPDGISSAMARIEQAAEGLECRRSLSNSAATLWHPEAHFDWVRPGIILYGASPS
                     GQWRDIANTGLRPVMTLSSEIIGVQTLKAGERVGYGGRYTARDEQRIGIVAAGYADGY
                     PRHAPTGTPVLVDGVRTMTVGTVSMDMLAVDLTPCPQAGIGTPVELWGKEIKIDDVAA
                     AAGTVGYELMCALALRVPVVTV"
     gene            complement(1367..2665)
                     /gene="dadA"
                     /locus_tag="ACHOSC_RS16935"
                     /old_locus_tag="pgaptmp_003387"
     CDS             complement(1367..2665)
                     /gene="dadA"
                     /locus_tag="ACHOSC_RS16935"
                     /old_locus_tag="pgaptmp_003387"
                     /EC_number="1.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-amino acid dehydrogenase"
                     /protein_id="HGU4549274.1"
                     /translation="MRVVILGSGVVGVASAWYLNQAGHEVTVIDREPGAALETSAANA
                     GQISPGYAAPWAAPGVPLKAIKWMFQRHAPLAVRLDGTQFQLKWMWQMLRNCDTSHYM
                     ENKGRMVRLAEYSRDCLKALRAETNIQYEGRQGGTLQLFRTEQQYENATRDIAVLEDA
                     GVPYQLLESSRLAEVEPALAEVAHKLTGGLQLPNDETGDCQLFTQNLARMAEQAGVKF
                     RFNTPVDQLLCDGEQIYGVKCGDEVIKADAYVMAFGSYSTAMLKGIVDIPVYPLKGYS
                     LTIPIAQEDGAPVSTILDETYKIAITRFDNRIRVGGMAEIVGFNTELLQPRRETLEMV
                     VRDLYPRGGHVEQATFWTGLRPMTPDGTPVVGRTRFKNLWLNTGHGTLGWTMACGSGQ
                     LLSDLLSGRTPAIPYEDLSVARYSRGFTPSRPGHLHGAHS"
     gene            2995..4527
                     /locus_tag="ACHOSC_RS16940"
                     /old_locus_tag="pgaptmp_003388"
     CDS             2995..4527
                     /locus_tag="ACHOSC_RS16940"
                     /old_locus_tag="pgaptmp_003388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpoVR family protein"
                     /protein_id="HGU4549275.1"
                     /translation="MATIDSMNKDTTRLSDGPDWTFDLLDVYLAEIDRVAKLYRLDTY
                     PHQIEVITSEQMMDAYSSVGMPINYPHWSFGKKFIETERLYKHGQQGLAYEIVINSNP
                     CIAYLMEENTITMQALVMAHACYGHNSFFKNNYLFRSWTDASSIVDYLIFARKYITEC
                     EERYGVDEVERLLDSCHALMNYGVDRYKRPQKISLQEEKARQKSREEYLQSQVNMLWR
                     TLPKREEEKTVAEARRYPSEPQENLLYFMEKNAPLLESWQREILRIVRKVSQYFYPQK
                     QTQVMNEGWATFWHYTILNHLYDEGKVTERFMLEFLHSHTNVVFQPPYNSPWYSGINP
                     YALGFAMFQDIKRICQSPTEEDKYWFPDIAGSDWLETLHFAMRDFKDESFISQFLSPK
                     VMRDFRFFTVLDDDRHNYLEISAIHNEEGYREIRNRLSSQYNLSNLEPNIQIWNVDLR
                     GDRSLTLRYIPHNRAPLDRGRKEVLKHVHRLWGFDVMLEQQNEDGSIELLERCPPRMG
                     NL"
     gene            complement(4579..5298)
                     /gene="fadR"
                     /locus_tag="ACHOSC_RS16945"
                     /old_locus_tag="pgaptmp_003389"
     CDS             complement(4579..5298)
                     /gene="fadR"
                     /locus_tag="ACHOSC_RS16945"
                     /old_locus_tag="pgaptmp_003389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000062 - fatty-acyl-CoA binding [Evidence
                     IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0019217 - regulation of fatty acid
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acid metabolism transcriptional regulator
                     FadR"
                     /protein_id="HGU4549276.1"
                     /translation="MVIKAQSPAGFAEEYIIESIWNNRFPPGTILPAERELSELIGVT
                     RTTLREVLQRLARDGWLTIQHGKPTKVNNFWETSGLNILETLARLDHESVPQLIDNLL
                     SVRTNISTIFIRTAFRQHPDKAQEVLATANEVADHADAFAELDYNIFRGLAFASGNPI
                     YGLILNGMKGLYTRIGRHYFANPEARSLALGFYHKLSALCSEGAHDQVYETVRRYGHE
                     SGEIWHRMQKNLPGDLAIQGR"
     gene            5520..7061
                     /gene="nhaB"
                     /locus_tag="ACHOSC_RS16950"
                     /old_locus_tag="pgaptmp_003390"
     CDS             5520..7061
                     /gene="nhaB"
                     /locus_tag="ACHOSC_RS16950"
                     /old_locus_tag="pgaptmp_003390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015385 - sodium:proton antiporter
                     activity [Evidence IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Na(+)/H(+) antiporter NhaB"
                     /protein_id="HGU4549277.1"
                     /translation="MEISWGRALWRNFLGQSPDWYKLALIIFLIVNPLIFLISPFVAG
                     WLLVAEFIFTLAMALKCYPLLPGGLLAIEAVFIGMTSAEHVREEVAANLEVLLLLMFM
                     VAGIYFMKQLLLFIFTRLLLSIRSKMLLSLSFCVAAAFLSAFLDALTVVAVVISVAVG
                     FYGIYHRVASSRTEDTDLQDDSHIDKHYKVVLEQFRGFLRSLMMHAGVGTALGGVMTM
                     VGEPQNLIIAKAAGWHFGDFFLRMSPVTVPVLICGLLTCLLVEKLRWFGYGETLPEKV
                     REVLQQFDDQSRHQRTRQDKIRLIVQAIIGVWLVTALALHLAEVGLIGLSVIILATSL
                     TGVTDEHAIGKAFTESLPFTALLTVFFSVVAVIIDQQLFSPIIQFVLQASEHAQLSLF
                     YIFNGLLSSISDNVFVGTIYINEAKAAMESGAITLKQYELLAVAINTGTNLPSVATPN
                     GQAAFLFLLTSALAPLIRLSYGRMVWMALPYTLVLTLVGLLCVEFTLAPVTEWFMQMG
                     WIATL"
     gene            7207..7737
                     /gene="dsbB"
                     /locus_tag="ACHOSC_RS16955"
                     /old_locus_tag="pgaptmp_003391"
     CDS             7207..7737
                     /gene="dsbB"
                     /locus_tag="ACHOSC_RS16955"
                     /old_locus_tag="pgaptmp_003391"
                     /EC_number="1.8.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707082.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015035 - protein-disulfide reductase
                     activity [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="disulfide bond formation protein DsbB"
                     /protein_id="HGU4549278.1"
                     /translation="MLRFLNQCSQGRGAWLLMAFTALALELTALWFQHVMLLKPCVLC
                     IYERCALFGVLGAALIGAIAPKTPLRYVAMVIWLYSAFRGVQLTYEHTMLQLYPSPFA
                     TCDFMVRFPEWLPLDKWVPQVFVASGDCAERQWDFLGMEMPQWLLGIFIAYLIVAVLV
                     VISQPFKAKKRDLFGR"
     gene            complement(7783..9050)
                     /gene="umuC"
                     /locus_tag="ACHOSC_RS16960"
                     /old_locus_tag="pgaptmp_003392"
                     /pseudo
     CDS             complement(7783..9050)
                     /gene="umuC"
                     /locus_tag="ACHOSC_RS16960"
                     /old_locus_tag="pgaptmp_003392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415702.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase V catalytic protein"
     gene            complement(9050..9469)
                     /locus_tag="ACHOSC_RS16965"
                     /old_locus_tag="pgaptmp_003393"
     CDS             complement(9050..9469)
                     /locus_tag="ACHOSC_RS16965"
                     /old_locus_tag="pgaptmp_003393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translesion error-prone DNA polymerase V
                     autoproteolytic subunit"
                     /protein_id="HGU4549279.1"
                     /translation="MLFIKPADLREIVTFPLFSDLVQCGFPSPAADYVEQRIDLNQLL
                     IQHPSATYFVKASGDSMIDGGISDGDLLIVDSAITASHGDIVIAAVDGEFTVKKLQLR
                     PTVQLIPMNSAYSPITISSEDTLDVFGVVIHVVKAMR"
BASE COUNT         2280 a         2583 c         2382 g         2411 t
ORIGIN      
        1 attgaacata tcaaaatcag gcaattacac aaatctatgt acaggctcca tcaagggtaa
       61 agcgtgattt atctgaagtc gagttcgagt caacaatgaa gcgtaagcgt tttatgtaga
      121 acgcctgtgc ggtgagttca ggttccgggg aaatagtgtc cattctttta atggacacta
      181 tcatgggcaa tggctctgtc acattaacaa ctacagttgc tgaccagccg gatggatgat
      241 tttttcgcaa ccagaagacg ctgcctccga tccggcttac aacaagttac actgtcacaa
      301 ccgggacgcg taacgccagc gcgcacatca actcatagcc caccgttccg gcagcggcgg
      361 cgacatcatc aattttgatc tccttgcccc acagctcaac cggcgtacca atccccgcct
      421 gcgggcaagg cgttaaatcg accgctagca tatccatcga gacggtcccc accgtcatgg
      481 tgcgcacgcc gtccactaaa acaggggtac cggtaggcgc gtggcgcgga taaccgtcgg
      541 cgtaccctgc ggcgacaatg ccgattcgct gttcatcgcg cgcagtatag cgaccgccgt
      601 agcccacacg ctcacccgct tttagcgtct ggacaccaat aatctcactg cttagcgtca
      661 tcaccggacg taatccggta ttggcgatat cacgccactg accggacggc gaagcgccat
      721 acaaaataat gccaggccga acccagtcaa aatgcgcttc cgggtgccac agagtcgccg
      781 ccgaattgga caacgaacgc cgacactcca gcccttccgc cgcctgctca atacgcgcca
      841 tcgcgctgga aattccatca ggatgttccg cttcggcaaa atgcgacatc agggtcatct
      901 cgccaacatt cgccattgcc cgcaactgct gccagacggt aagcacgcga tcgggctgga
      961 agcccaaccg attcatccca ctgttcactt taagataaat atccaacggt gcttttagcc
     1021 gcgcattttg cagtgctttg agctgccagt tgctgtgtac gcaggtggtc aggcggtgct
     1081 ggtcataaat ctccagatcc tgagcatgga aaaatccttc cagcatcagg atcggcccct
     1141 tccagccgcg ctcacgtaac gttattgcct cttccaggtt aagtaatgca aagccatcgg
     1201 tggccccgag cgcgctccag atacgctcaa taccatgccc gtaagcgttc gcttttacca
     1261 ccgaccagac gcgcgcgtgc ggcgcggcct ggcggacaat ggacagattc tgttttaatg
     1321 cctgcagatc gaggctggcc tgtatcggac gggtcatctc gtttccttag ctgtgtgcgc
     1381 catgtaaatg gcccggacgt gatggcgtaa atccacggct gtagcgcgct acgcttagat
     1441 cctcatatgg gatcgctggc gtgcgaccag agagcagatc gcttaacaac tgaccggaac
     1501 cgcaagccat cgtccagccg agcgtgccgt gaccggtatt cagccacaga tttttaaagc
     1561 gtgtacgccc gacaaccggc gtaccgtctg gcgtcatcgg gcgcaaaccc gtccagaaag
     1621 tcgcctgctc gacatgaccg ccgcgtggat agagatcgcg aaccaccatt tccagcgttt
     1681 cacgacgcgg ttgcaacagc tcggtgttaa aaccaacaat ttccgccatt ccgccaacac
     1741 gaatgcggtt atcgaaacgg gtaatggcga ttttgtaggt ttcatcaaga atagtggata
     1801 ctggcgcacc atcttcctgc gcaattggaa tagtcagcga gtagcctttc agcggataca
     1861 ccggaatatc aacaatgcct ttgagcatcg ccgtcgagta agaaccaaac gccatcacat
     1921 acgcatcggc cttaatcact tcatcgccac acttcacgcc gtagatttgc tcgccgtcgc
     1981 aaagcagttg gtcaacgggc gtattaaagc ggaatttaac ccccgcctgc tctgccatcc
     2041 gcgccagatt ctgggtaaac agctggcagt ctccggtttc atcattgggc agttgcagac
     2101 cacccgtcag tttgtgcgct acttctgcca gcgcgggctc cacttccgcc aggcggctgg
     2161 attccagcag ctgatacggt acgccggcat cttccagcac ggcgatatcg cgggtcgcat
     2221 tttcatactg ttgttcggta cggaacagtt gcagcgtccc accctgacgc ccttcatact
     2281 gaatattggt ttcggcgcgc aatgctttca ggcaatcacg gctgtattcc gccagacgca
     2341 ccatccgccc tttgttttcc atgtagtggc tggtgtcgca gttacgtaac atttgccaca
     2401 tccatttcaa ctgaaactgc gtaccgtcga gacggaccgc cagcggcgca tggcgctgga
     2461 acatccattt gatcgctttt aaaggcacac ctggtgccgc ccacggtgca gcatatccgg
     2521 gggagatttg cccggcattc gcggcgctgg tttccagggc ggcccccggc tcccgatcaa
     2581 tgacggtgac ctcatgtcct gcctgattta agtaccaggc gctggcaacg cctaccacac
     2641 cacttcccag tatgacaact cgcatagcca ctccgataac ggtagataca gtaaaagaat
     2701 aataatctaa ttacatcttg ataactcagt tgaaaatatt attcaacata cggctttttt
     2761 atggtgagct ggctcacatc tccctgactc tgcggcaata tggcgtcttt agtatctcct
     2821 gctgaacgcc ctttttatcc agcataaaat tccgttcaga agcggattag tggcactctg
     2881 ataataagaa atcgcaaaga aaaaaattct ccggcgacgc ggtttttaac aggtgttcta
     2941 tgcttgaaat gaggtgctcc acacagagag cgccgacaac aatgaggtgc gcgtatggcg
     3001 acgatcgatt ctatgaataa ggacaccaca cgtttgagcg atggacccga ctggacgttc
     3061 gacctgctgg atgtttatct ggcagagata gaccgggtgg cgaaactcta ccggctggat
     3121 acctacccgc accagattga agtgataacc tcagaacaga tgatggatgc ctactccagc
     3181 gtcggcatgc caattaacta tccgcactgg tcattcggta aaaagtttat cgaaactgaa
     3241 cggctgtata agcacggtca gcaaggactg gcctatgaaa tcgtcattaa ctctaacccg
     3301 tgtatcgctt acctgatgga agagaacacc attaccatgc aggcgctggt gatggcacac
     3361 gcctgctatg ggcataactc tttctttaaa aacaattact tattccgcag ttggaccgac
     3421 gccagttcga ttgtcgatta tctgattttt gcccgtaaat atattaccga gtgcgaagaa
     3481 cgttatggcg ttgatgaagt agaacggctt ctggactcgt gccacgcgct gatgaactac
     3541 ggcgtggacc ggtacaaacg cccgcaaaaa atctcactgc aagaagagaa agcccggcag
     3601 aaaagtcgcg aagagtatct acaaagtcag gtcaatatgc tctggcgtac cctgccgaag
     3661 cgcgaggaag agaaaacggt tgctgaagcg cgccgctatc cgtccgaacc acaagaaaac
     3721 ctgctctatt ttatggagaa aaatgcgcca ctgctggaat catggcagcg tgaaatcctg
     3781 cgtattgtgc gtaaggtgag ccagtatttt tatccgcaaa aacagactca ggtgatgaac
     3841 gaaggctggg cgaccttctg gcactatacc atccttaacc atctgtatga tgaagggaaa
     3901 gtaacggaac gttttatgct ggagtttttg cacagccaca ccaatgtggt cttccagccc
     3961 ccctataaca gcccgtggta cagcggcatc aacccgtatg ccctcgggtt cgccatgttc
     4021 caggatatta aacggatttg tcagtcgcca acggaagaag acaaatactg gttcccggat
     4081 atcgccggtt ccgactggct ggaaacgctg catttcgcga tgcgtgattt caaagatgag
     4141 agttttatca gccagtttct gtcaccgaaa gtgatgcgtg atttccgctt cttcaccgtg
     4201 ctggatgatg atcggcataa ttatctggag atttccgcta ttcataatga agaaggttat
     4261 cgggagatcc gtaaccggtt atcgtcgcaa tataacttaa gtaatctgga gccgaatatt
     4321 cagatctgga acgtggattt gcgcggcgac cgttcgctga cgctgcgtta cattccacat
     4381 aatcgcgcac cgctggatcg ggggcgcaaa gaagtgctga agcatgtgca tcgcctgtgg
     4441 ggatttgatg tgatgctcga acagcaaaac gaagacggca gcatcgagtt gctggaacgt
     4501 tgcccgccaa gaatgggaaa tctgtaaaaa caacaaaaaa cccctcgttt gaggggtttg
     4561 ctctttaaac ggaagggatt atcgcccctg aatggctaaa tcacccggca gatttttctg
     4621 catccggtgc caaatctcgc cactctcatg cccatagcga cgcactgttt cgtacacctg
     4681 atcgtgcgcg ccttcactgc acaacgccga cagtttgtgg tagaagccca gcgccagact
     4741 gcgcgcttcc ggattggcga aatagtgacg accaatgcgc gtatacagcc ctttcatccc
     4801 gttaaggatc agaccgtaaa tcgggttgcc ggaagcaaac gccaggccgc ggaatatgtt
     4861 gtaatccagc tcggcaaagg catcggcgtg atcggccact tcattagcgg tagccagcac
     4921 ttcctgcgct ttatcgggat gctgacgaaa cgcggtgcga ataaaaatag tggaaatatt
     4981 ggtacgcacc gacagcaaat tatcaataag ctgcggcaca ctttcgtgat ccagtcgcgc
     5041 cagtgtttca aggatattta aaccagaggt ttcccagaaa ttattcacct tcgtcggctt
     5101 gccatgttga atggtcaacc agccatctcg tgccagacgc tgtaacactt cacgtaacgt
     5161 agtacgcgtt acgccaatta attctgaaag ttcacgttct gcgggcaaaa tagtcccggg
     5221 agggaagcgg ttattccaga tactttcaat aatgtactct tccgcgaaac ccgccgggct
     5281 ttgcgcctta atgaccatag tgagatttcc ataacacagc aaaacaaagt tggactcatc
     5341 ataccagagg gctaactacg ctgatagcag acaaaaagaa gaaaaaaggg atcaaggctt
     5401 cattttccgc atctctttcc ccttgactat caagacttgc ctcactcctg cctgaccgtt
     5461 aagctttgcg tttaaacata aaaacatcat tgattatttg taaggtaagg gaaaccatca
     5521 tggagatctc ctggggccgc gcgctatggc gcaatttttt gggccagtcc cccgactggt
     5581 acaaactcgc cctcatcatt ttcttaatcg taaacccgtt aattttcctc atcagccctt
     5641 tcgtcgcggg ctggttgctg gtagcggaat ttattttcac tctggcgatg gccctgaaat
     5701 gctacccgct gctccccggt ggtctgttgg ctatcgaagc ggtattcatc ggcatgacca
     5761 gcgcggaaca cgtccgtgaa gaggtggcgg caaatcttga agtcttgctg ttattgatgt
     5821 ttatggtggc gggtatctat tttatgaaac agctgttgct gttcatattt acccgtttgc
     5881 tgttaagcat tcgctccaaa atgctgctgt cgctctcttt ttgcgtggcg gcggcgttcc
     5941 tctccgcgtt cctcgatgcc ttaaccgtcg tggcggtggt gatcagcgtt gcagtcggtt
     6001 tttatggtat ttatcaccgc gtagcctctt cccgtactga agataccgac ctgcaagacg
     6061 atagtcatat cgacaagcat tacaaagtgg ttctggaaca gttccgtggc tttctgcgta
     6121 gcctgatgat gcatgccggt gtcggcactg cattaggcgg cgtgatgacc atggtgggcg
     6181 aaccacagaa cctgatcatc gctaaagcgg ctggctggca ttttggcgat ttcttcctgc
     6241 gcatgtcgcc ggtgaccgtt ccggttctga tttgtggcct gttaacctgc ctgctggtag
     6301 agaagctgcg ttggtttggc tacggtgaaa cgctgccgga gaaagtccgc gaagtgttgc
     6361 aacagtttga cgatcaaagc cgccaccagc gtactcgtca ggataaaatc cgtctgattg
     6421 tccaggcgat tattggcgtc tggctggtga ctgcgctggc gttgcatctg gcggaagttg
     6481 gcttgattgg tttgtcagtc attattctgg cgacatcatt gaccggtgtc accgatgaac
     6541 atgctatcgg taaagccttc accgaatctc tgccattcac cgcactgttg acggtgtttt
     6601 tctcggtagt cgcggtgatt atcgaccaac aactgttttc gccaattatt cagtttgtgt
     6661 tgcaggcatc ggaacatgct cagctgtcgc tgttctatat tttcaacggt ctgctgtcat
     6721 ccatttcgga taacgtcttc gtggggacga tttatatcaa cgaagcgaaa gcggcaatgg
     6781 aaagtggcgc tatcacgttg aagcaatacg agctgctggc ggtcgccatt aataccggta
     6841 ccaatctgcc ctccgtcgct acgccgaacg gtcaggctgc gttcctgttc ctgctgacct
     6901 ctgcactcgc gccattgatt cgcctctctt atggccgcat ggtgtggatg gccctgcctt
     6961 acaccctcgt actgacactc gtcggcttgc tctgcgtcga gtttacgctt gcccctgtaa
     7021 ccgaatggtt tatgcaaatg ggctggatag caacgctttg ataacaactt accgggcata
     7081 taaatgcccg gtttgccttt tcgcccgata attgtccaat tgcgtcctat ttttgctgcc
     7141 tcctggtggc gcagcgaatg aattggttta aactgcgcac tctatgcata ttgcagggaa
     7201 atgattatgt tgcgattttt gaaccaatgt tcacaaggcc ggggcgcgtg gttgttgatg
     7261 gcgtttactg ctctggcact ggaactgacg gcgctgtggt tccagcatgt gatgttactg
     7321 aaaccttgcg tgctctgtat ttatgaacgc tgcgcgttat tcggcgttct gggtgctgcg
     7381 ctgattggcg cgatcgcccc gaaaactccg ctgcgttatg tagcgatggt tatctggttg
     7441 tatagtgcgt tccgcggtgt gcagttaact tacgagcaca ccatgcttca gctctatcct
     7501 tcgccgtttg ccacctgtga ttttatggtt cgtttcccgg aatggctgcc gctggataaa
     7561 tgggtgccgc aagtgtttgt cgcctctggc gattgcgccg agcgtcagtg ggatttttta
     7621 ggtatggaaa tgccgcagtg gctgctcggt atttttatcg cttacctgat tgtcgcggtg
     7681 ctggtggtga tttcccagcc gtttaaagcg aaaaaacgtg acctgttcgg tcgctaatcc
     7741 attcggcgct cctgcgggag cgcttttttc ctgccgctat atttatttga cccgcagtaa
     7801 atcagaactt cgcgttgtat aacgtggtga aagcatggca cgcttcatct gccattgttg
     7861 ctggatcccc tgcccggcaa aatagagtgt tcctctgccc tctttagcat tcagtgtatc
     7921 cattaccgtc atcaattgct cactcccggg gcgcggtgcg ttgtcatcga ataaattgag
     7981 ctgcgcgact ccctgactga agaaatcccc cagcatcacg cccgcttttt ggtagcgatg
     8041 gcccgcttgc cagatggcat ccagagatcg cgtagcagcg ttaatgatat ccctgctgtc
     8101 ctgagtgggc gtcagcagtt ttaccgacgc gctattgccg taataaggtt cattgagcgc
     8161 aaatggtgac gtcttaataa atgtggagat aaaccgacaa tattgatgct cgctgcgaag
     8221 tttttccgcc gcccgggcag cgtaactaca aatggcctgc cgcatcgatg gataatccgt
     8281 gatgcgttca ccaaacgagc gggaacagat aatttcctgc ttcgtcggtg caaactcttc
     8341 cagttgcaaa cagggttcgc cgcgcagttc acgcaccgtt ctttcgagca cgacattaaa
     8401 atgtttacgg ataaaccgga tatctgtatc cgccaaatca agaacggttt tgatccccat
     8461 cgcgtccagt tttttgctga tccgccgtcc aatcccccag acttcatcca cggggagagc
     8521 agacattaat ttacgctggc gttccagatt tgataaatcc accacccccc cgtctgccgc
     8581 tgccattttt ttgccgcatg attggcaagc ttagccagcg ttttggtctg ggcgatcccc
     8641 acaccaacag taagatgggt acgttgtagc accgttgcgc gaatttctct gccaaaatca
     8701 gtcagatcgc gacaattacg cacacctgtc agatcgcaga atgcctcatc aatactgtaa
     8761 atctcgacgc ggggcgatag ttcttccagc gtcgacatca cccgattgct catgtctgcg
     8821 taaagctcat aattgctgct aaagcaaacc acaccacagc gacgaaacag atctttttgt
     8881 ttgaaccagg gatcgcccat tttaacaccc agcgcctttg cctcagcgtt tcgggcgata
     8941 acgcaaccgt cattattcga tagcacaacc accggtttac cccataaatc agggcgaaac
     9001 accgtctcac agctggcata aaacgcgttt acatcacaga gagcaaacat cagcgcatcg
     9061 ccttaacgac gtggatcacc acaccaaaga catccagcgt atcttcgcta ctgatggtaa
     9121 tgggcgagta cgcactgttc atgggaataa gctgtaccgt cgggcgtagt tgcaattttt
     9181 tcaccgtaaa ctcgccgtca acagcagcga tgacaatatc accatggctg gcggtaatag
     9241 cgctatcgac aatcagtaaa tcaccgtcac taattccacc atcaatcata gaatcaccac
     9301 ttgctttgac gaagtaagtc gcgctgggat gctggatcaa cagttgattc agatcgatgc
     9361 gctgttcaac gtaatctgct gccggtgaag gaaagccaca ctgaacaaga tcgctaaata
     9421 gcggaaaagt cacaatttcg cggagatccg caggcttgat aaacaacata ataatctgcc
     9481 tgaagctata ctgtttttat atacagtagt ctgttcttgc cagcagatca atactgattc
     9541 aggctatcaa tatttgtcgc tgcatagact gctgattttt cgttctctta tcctgtgctc
     9601 acgcggcctt ctggcgacta cgctcatcca gcagaaatga aaaatattac ccggct
//
LOCUS       DAZKHF020000174         9631 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2201S31241
            SAMN43267800-rid25106413.denovo.175, whole genome shotgun sequence.
ACCESSION   DAZKHF020000174 DAZKHF020000000
VERSION     DAZKHF020000174.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267800
            Sequence Read Archive: SRR30307263
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 9631)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9631)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 9631)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350581.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350581.2
            Genome Coverage       :: 151x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:21:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,652
            CDSs (total)                      :: 5,557
            Genes (coding)                    :: 5,320
            CDSs (with protein)               :: 5,320
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 237
            CDSs (without protein)            :: 237
            Pseudo Genes (ambiguous residues) :: 0 of 237
            Pseudo Genes (frameshifted)       :: 102 of 237
            Pseudo Genes (incomplete)         :: 130 of 237
            Pseudo Genes (internal stop)      :: 52 of 237
            Pseudo Genes (multiple problems)  :: 41 of 237
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9631
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267800-rid25106413.denovo.175"
                     /serotype="O 26:H11"
                     /isolate="2201S31241"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-01"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            128..664
                     /gene="hpt"
                     /locus_tag="ACHOSC_RS16970"
                     /old_locus_tag="pgaptmp_003394"
     CDS             128..664
                     /gene="hpt"
                     /locus_tag="ACHOSC_RS16970"
                     /old_locus_tag="pgaptmp_003394"
                     /EC_number="2.4.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004204384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006166 - purine ribonucleoside salvage
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hypoxanthine phosphoribosyltransferase"
                     /protein_id="HGU4549280.1"
                     /translation="MKHTVEVMIPEAEIKARIAELGRQITERYKDSGSDMVLVGLLRG
                     SFMFMADLCREVQVSHEVDFMTASSYGSGMSTTRDVKILKDLDEDIRGKDVLIVEDII
                     DSGNTLSKVREILSLREPKSLAICTLLDKPSRREVNVPVEFIGFSIPDEFVVGYGIDY
                     AQRYRHLPYIGKVILLDE"
     gene            complement(705..1367)
                     /gene="can"
                     /locus_tag="ACHOSC_RS16975"
                     /old_locus_tag="pgaptmp_003395"
     CDS             complement(705..1367)
                     /gene="can"
                     /locus_tag="ACHOSC_RS16975"
                     /old_locus_tag="pgaptmp_003395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004089 - carbonate dehydratase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0015976 - carbon utilization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbonate dehydratase"
                     /protein_id="HGU4549281.1"
                     /translation="MKDIDTLISNNALWSKMLVEEDPGFFEKLAQAQKPRFLWIGCSD
                     SRVPAERLTGLEPGELFVHRNVANLVIHTDLNCLSVVQYAVDVLEVEHIIICGHYGCG
                     GVQAAVENPELGLINNWLLHIRDIWFKHSSLLGEMPQERRLDTLCELNVMEQVYNLGH
                     STIMQSAWKRGQKVTIHGWAYGIHDGLLRDLDVTATNRETLEQRYRHGISNLKLKHAN
                     HK"
     gene            1476..2402
                     /locus_tag="ACHOSC_RS16980"
                     /old_locus_tag="pgaptmp_003396"
     CDS             1476..2402
                     /locus_tag="ACHOSC_RS16980"
                     /old_locus_tag="pgaptmp_003396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HGU4549282.1"
                     /translation="MTIALELQQLKKTYPGGVQALRGIDLQVEAGDFYALLGPNGAGK
                     STTIGIISSLVNKTSGRVSVFGYDLEKDVVNAKRQLGLVPQEFNFNPFETVQQIVVNQ
                     AGYYGVERKEAYIRSEKYLKQLDLWGKRNERARMLSGGMKRRLMIARALMHEPKLLIL
                     DEPTAGVDIELRRSMWGFLKDLNDKGTTIILTTHYLEEAEMLCRNIGIIQHSELVENT
                     SMKALLAKLKSETFILDLAPKSPLPKLDGYQYRLVDTATLEVEVLREQGINSVFTQLS
                     EQGIQVLSMRNKANRLEELFVSLVNEKQGDRA"
     gene            2399..3169
                     /locus_tag="ACHOSC_RS16985"
                     /old_locus_tag="pgaptmp_003397"
     CDS             2399..3169
                     /locus_tag="ACHOSC_RS16985"
                     /old_locus_tag="pgaptmp_003397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001747261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HGU4549283.1"
                     /translation="MMHLYWVALKSIWAKEIHRFMRIWVQTLVPPVITMTLYFIIFGN
                     LIGSRIGDMHGFSYMQFIVPGLIMMSVITNAYANVASSFFGAKFQRNIEELLVAPVPT
                     HVIIAGYVGGGVARGLFVGILVTAISLFFVPFQVHSWVFVALTLVLTAVLFSLAGLLN
                     GVFAKTFDDISLVPTFVLTPLTYLGGVFYSLTLLPPFWQGLSHLNPIVYMISGFRYGF
                     LGINDVPLVTTFGVLVVFIVAFYLICWSLIQRGRGLRS"
     gene            3274..3714
                     /locus_tag="ACHOSC_RS16990"
                     /old_locus_tag="pgaptmp_003398"
     CDS             3274..3714
                     /locus_tag="ACHOSC_RS16990"
                     /old_locus_tag="pgaptmp_003398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HGU4549284.1"
                     /translation="MLGWVITCHDDRAQEILDALEKKHGALLQCRAVNFWRGLSSNML
                     SRMMCDALHEADSGEGVIFLTDLAGAPPYRVASLLSHKHSRCEVISGVTLPLIEQMMA
                     CRETMTSSEFRERIVELGGPEVSSLWHQQQKNPPFVLKHNLYEY"
     gene            3778..5007
                     /locus_tag="ACHOSC_RS16995"
                     /old_locus_tag="pgaptmp_003399"
     CDS             3778..5007
                     /locus_tag="ACHOSC_RS16995"
                     /old_locus_tag="pgaptmp_003399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HGU4549285.1"
                     /translation="MYKQAVILLLMLFTASVSAALPARYMQTIENAAIWAQIGDKMVT
                     VGNIRAGQIIAVEPTAASYYAFNFGFGKGFIDKGHLEPVQGRQKVEDGLGDLNKPLSN
                     QNLITWKDTPVYNAPSAGSAPFGVLADNLRYPILHKLKDRLNQTWYQIRIGDRLAYIS
                     ALDAQPDNGLPVLTYHHILRDEENTRFRHTSTTTSVRAFNNQMAWLRDRGYATLSMAQ
                     LEGYVKNKINLPARAVVITFDDGLKSVSRYAYPVLKQYGMKATAFVVTSRIKRHPQKW
                     NPKSLQFMSVSELNEIRDVFDFQSHTHFLHRVDGYRRPILLSRSEHNILFDFARSRRA
                     LAQFNPHVWYLSYPFGGFNDKAVKAANDAGFHLAVTTMKGKVKPGDNPLLLKRLYILR
                     TDSLETMSRLVSNQPQG"
     gene            complement(5011..5391)
                     /gene="panD"
                     /locus_tag="ACHOSC_RS17000"
                     /old_locus_tag="pgaptmp_003400"
     CDS             complement(5011..5391)
                     /gene="panD"
                     /locus_tag="ACHOSC_RS17000"
                     /old_locus_tag="pgaptmp_003400"
                     /EC_number="4.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004068 - aspartate 1-decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006523 - alanine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate 1-decarboxylase"
                     /protein_id="HGU4549286.1"
                     /translation="MIRTMLQGKLHRVKVTHADLHYEGSCAIDQDFLDAAGILENEAI
                     DIWNVTNGKRFSTYAIAAERGSRIISVNGAAAHCASVGDIVIIASFVTMPDEEARTWR
                     PNVAYFEGDNEMKRTAKAIPVQVA"
     gene            5665..6567
                     /gene="rpnC"
                     /locus_tag="ACHOSC_RS17005"
                     /old_locus_tag="pgaptmp_003401"
     CDS             5665..6567
                     /gene="rpnC"
                     /locus_tag="ACHOSC_RS17005"
                     /old_locus_tag="pgaptmp_003401"
                     /EC_number="3.1.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination-promoting nuclease RpnC"
                     /protein_id="HGU4549287.1"
                     /translation="MDAPSTTPHDAVFKQFLMHAETARDFLEIHLPVELRELCDLNTL
                     HLESGSFIEESLKGHSTDVLYSVQMQGNPGYLHVVIEHQSKPDKKMAFRMMRYSIAAM
                     HRHLEADHDKLPLVVPILFYQGEATPYPLSMCWFDIFYSPELARRVYNSPFPLVDITI
                     TPDDEIMQHRRIAILELLQKHIRQRDLMLLLEQLVTLIDEGYTSGSQLVAMQNYMLQR
                     GHTEQADLFYGVLRDRETGGESMMTLAQWFEEKGIEKGIQQGRQEERQEFALRLLSKG
                     MSREDVAEMANLPLAEIDKVINLI"
     gene            complement(6641..7492)
                     /gene="panC"
                     /locus_tag="ACHOSC_RS17010"
                     /old_locus_tag="pgaptmp_003402"
     CDS             complement(6641..7492)
                     /gene="panC"
                     /locus_tag="ACHOSC_RS17010"
                     /old_locus_tag="pgaptmp_003402"
                     /EC_number="6.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004592 - pantoate-beta-alanine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pantoate--beta-alanine ligase"
                     /protein_id="HGU4549288.1"
                     /translation="MLIIETLPLLRQQIRRLRMEGKRVALVPTMGNLHDGHMKLVDEA
                     KARADVVVVSIFVNPMQFDRPEDLARYPRTLQEDCEKLNKRKVDLVFAPSVKEIYPNG
                     TETHTYVDVPGLSTMLEGASRPGHFRGVSTIVSKLFNLVQPDIACFGEKDFQQLALIR
                     KMVADMGFDIEIVGVPIIRAKDGLALSSRNGYLTAEQRKIAPGLYKVLSSIADKLQAG
                     ERDLDEIITIAGQELNEKGFRADDIQIRDADTLLEVSETSKRAVILVAAWLGDARLID
                     NKMVELA"
     gene            complement(7504..8298)
                     /gene="panB"
                     /locus_tag="ACHOSC_RS17015"
                     /old_locus_tag="pgaptmp_003403"
     CDS             complement(7504..8298)
                     /gene="panB"
                     /locus_tag="ACHOSC_RS17015"
                     /old_locus_tag="pgaptmp_003403"
                     /EC_number="2.1.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000805487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003864 - 3-methyl-2-oxobutanoate
                     hydroxymethyltransferase activity [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-methyl-2-oxobutanoate
                     hydroxymethyltransferase"
                     /protein_id="HGU4549289.1"
                     /translation="MKPTTIASLQKCKQDKKRFATITAYDYSFAKLFADEGLNVMLVG
                     DSLGMTVQGHDSTLPVTVADIAYHTAAVRRGAPNCLLLADLPFMAYATPEQAFENAAT
                     VMRAGANMVKIEGGEWLVETVKMLTERAVPVCGHLGLTPQSVNIFGGYKVQGRGDEAG
                     DQLLSDALALEAAGAQLLVLECVPVELAKRITDALAIPVIGIGAGNVTDGQILVMHDA
                     FGITGGHIPKFAKNFLAETGDIRAAVRQYMAEVESGVYPGEEHSFH"
     gene            complement(8411..9631)
                     /locus_tag="ACHOSC_RS17020"
                     /old_locus_tag="pgaptmp_003404"
                     /pseudo
     CDS             complement(8411..9631)
                     /locus_tag="ACHOSC_RS17020"
                     /old_locus_tag="pgaptmp_003404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001462196.1"
                     /note="internal stop; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial-like adhesin"
BASE COUNT         2369 a         2242 c         2467 g         2553 t
ORIGIN      
        1 ttcgagcttt attaacagat tccgcgaatg aatagtttta ctggtatact gcgtgtcttg
       61 cgctttgttg cggtgccaaa acctgcccgt gcgaagtgat ttgtttttaa atcatatggt
      121 tagagatatg aaacatactg tagaagtaat gatccccgaa gcggagatta aagcgcgtat
      181 cgccgaactg ggtcgtcaga ttactgagcg ttacaaagac agcggcagcg atatggtgct
      241 ggtgggtctg ctgcgtggct catttatgtt tatggcggac ctgtgccgtg aagttcaggt
      301 atctcatgaa gtcgacttta tgaccgcctc cagctacggt agcggcatgt ccaccacccg
      361 tgatgtgaaa atcctcaaag atctggatga agatatccgt ggcaaggacg tgctgattgt
      421 tgaagatatc atcgactcgg ggaatacact gtcgaaagtg cgtgagatct taagcctgcg
      481 cgaaccgaag tcgctggcga tttgtacgct gctggataaa ccgtcccgtc gtgaagtgaa
      541 cgtcccggta gaatttatcg gtttctcgat cccggatgag tttgtggtgg gttacggcat
      601 tgattacgca cagcgttacc gtcatctgcc gtatatcggc aaagtgattc tgctggacga
      661 gtaagtgtga agttgccgga tgtgttgcat ccggcatggc atttttattt gtggttggcg
      721 tgtttcagct tgaggttgga aatcccgtga cggtaacgtt gctcaagggt ttcgcggttg
      781 gtggcggtaa catccagatc acgcagcaag ccgtcgtgaa tgccgtaggc ccagccgtga
      841 atggtaactt tctgcccgcg tttccacgct gattgcataa tggtggagtg gcccaggtta
      901 tacacctgtt ccatgacgtt cagttcacac aaggtatcca gacggcgctc ttgcggcatt
      961 tcgccgagca atgagctatg tttgaaccag atatcgcgga tatgcagcag ccagttgttg
     1021 ataagcccca gttccgggtt ttcaactgcg gcttgtacgc cgccgcaacc gtagtggcca
     1081 cagataataa tgtgttcaac ttcgagtaca tccactgcat actgaaccac ggaaaggcag
     1141 ttcaggtcag tgtgaatgac caggttagca acattacggt gaacaaagag ttcgcccggc
     1201 tcaagaccgg ttaaacgttc tgcaggaacg cgactgtcgg aacatccaat ccatagaaag
     1261 cgcggttttt gcgcttgtgc cagtttctca aaaaacccgg gatcctcttc caccagcatt
     1321 tttgaccata gtgcattgtt gctgatgagt gtatctatgt ctttcatgga ggttaacgac
     1381 ctgtaaccaa ataattacgt ttggctaata tagggcaact ccgggacgat ttaaaccaca
     1441 gataaagtgt aagaacgtaa ggtaagtaaa aatttatgac cattgcactg gaacttcaac
     1501 agcttaaaaa aacctatcca ggcggcgttc aggcgcttcg tgggatagat ttgcaggtcg
     1561 aagcgggtga tttttatgcg cttctcgggc cgaacggggc cgggaaatcg accactatcg
     1621 gtattatcag ctctctggta aataaaacct ccgggcgggt cagcgtattt ggttacgatc
     1681 tcgagaagga tgtcgtgaac gctaaacgtc agttgggact ggtgccgcag gaatttaact
     1741 tcaacccgtt tgaaaccgtg cagcaaattg tggtgaatca ggcagggtac tacggtgtgg
     1801 agcgcaaaga ggcgtacatc cgcagcgaaa aatatcttaa gcagctcgat ctatggggaa
     1861 aacgcaacga acgtgcgcgt atgttatctg gcgggatgaa gcgccgttta atgattgccc
     1921 gtgcgttaat gcatgaacct aaactactga ttctcgacga accgaccgca ggcgtggata
     1981 ttgaacttcg ccgctcaatg tggggctttt tgaaggattt aaacgacaaa ggcaccacca
     2041 tcattctcac cacacactac ctggaagaag cagaaatgct gtgccgcaat atcggcatta
     2101 ttcaacacag tgagctggtg gaaaatacct cgatgaaggc gctgctggct aagctgaaat
     2161 cggaaacctt tattctcgat ctcgcaccga aaagtccgtt accgaagctc gatggctatc
     2221 agtatcgact ggtcgatacc gcgacgctgg aagttgaagt gctgcgtgag caggggatca
     2281 acagcgtatt tacgcagtta agtgagcagg gcattcaggt attaagtatg cgtaacaaag
     2341 ctaaccgtct ggaagagctg tttgtttcac tggttaatga aaaacaagga gatcgcgcat
     2401 gatgcatctt tactgggtgg cgctaaaaag catctgggcg aaagagatcc atcgctttat
     2461 gcgtatctgg gtgcagacgc tggtgccgcc agtcatcacc atgacccttt actttattat
     2521 cttcggtaac ctgataggtt cgcgtattgg cgatatgcat ggcttcagct atatgcagtt
     2581 catcgtaccg gggctgatca tgatgtcggt gatcaccaat gcctacgcta acgtcgcgtc
     2641 atcatttttt ggtgccaagt tccagcgtaa tattgaagag ctgctggtag cgccggttcc
     2701 gactcacgtc attattgccg gatatgtcgg cggtggcgtg gcgcgtggtc tgtttgttgg
     2761 cattctggtg acggcaattt cactgttttt tgtgccgttt caggtgcatt cgtgggtatt
     2821 cgttgcctta accctggtgc tcacggcggt gctattctcc cttgcgggtt tgctgaacgg
     2881 cgtgtttgcc aaaacgttcg atgacatcag cctggtgcca acctttgtgt taacgccact
     2941 cacgtatttg ggcggggtgt tttactccct gactttgttg ccgccgttct ggcaagggct
     3001 gtcgcacctg aacccaatcg tttatatgat cagtggtttc cgctacggct tcctcggtat
     3061 aaatgatgtg ccgctggtca ctacctttgg cgtactggtg gtctttattg tggcgtttta
     3121 tttgatctgt tggtcgctga tccaacgtgg acgtggtttg cgtagctaag gctatttcct
     3181 ctcctctgga ttcgggggag aggagttttg acggctatca ccctttatca acaatggtca
     3241 gggtagactg attttcggct aaggaggaag gcgatgttag gttgggtaat tacctgtcac
     3301 gatgaccggg cgcaagagat actggatgcg ctggagaaaa aacatggggc acttcttcag
     3361 tgccgggccg tgaatttctg gcgcggatta agctctaata tgctcagccg catgatgtgc
     3421 gatgctctgc atgaagcgga ctctggtgag ggtgtcatct tcttaaccga tttagccgga
     3481 gcgccaccgt accgcgtggc ttcattatta agccataaac actcccgttg tgaagtgatt
     3541 tctggtgtca cgttaccgtt aattgaacag atgatggctt gccgtgaaac catgaccagt
     3601 tcagagtttc gcgagcgtat tgtcgaactg ggtgggccgg aggtgagtag tctttggcac
     3661 caacaacaaa aaaatccgcc tttcgtcctc aaacataatt tgtatgagta ttaacccacg
     3721 attcttatag cgcttttgct acaataaaag cgttgtttca ccctcggtta ttttttcatg
     3781 tacaaacaag ctgttattct cctgctgatg ctgtttaccg caagtgtcag tgccgcgtta
     3841 cctgcccgtt atatgcaaac catcgaaaat gctgcgatct gggcgcaaat tggcgacaag
     3901 atggtgaccg tggggaatat tcgggccggg caaatcattg ccgtggagcc cactgccgca
     3961 agttattacg catttaattt tggctttggt aaagggttta tcgataaagg gcatctcgag
     4021 ccagttcagg ggcgacaaaa agttgaagac ggtttgggtg acctcaacaa gccgctgagt
     4081 aatcagaact taattacctg gaaagatacg ccggtctata acgcgccgag tgcgggcagt
     4141 gcgccatttg gggtactggc ggacaatttg cgctacccga ttttgcataa actgaaagac
     4201 aggttaaatc aaacctggta tcagatccgt attggcgatc gactcgccta tatcagcgcg
     4261 ctggatgccc aacccgataa tggcctgccg gtgctaacct atcaccatat tctgcgcgat
     4321 gaagaaaaca cccgttttcg ccatacttcg acgaccacct cggtacgcgc tttcaataac
     4381 caaatggcct ggctgcgcga caggggatac gcgacactga gcatggcgca gctggaaggt
     4441 tacgtgaaga ataagatcaa tctccctgcg cgagcggtgg tgattacctt tgatgatggc
     4501 ctcaagtcgg tgagccgcta tgcgtatcct gtattgaaac aatatggcat gaaggcgacg
     4561 gcgtttgttg ttacctcgcg catcaaacgt cacccgcaga agtggaaccc aaaatcgctg
     4621 caatttatga gcgtttctga gcttaacgaa attcgcgatg tttttgattt tcagtcacat
     4681 acccattttt tgcatcgggt ggatggctat cgccgaccca tattactgag ccgtagtgag
     4741 cacaatattc tgtttgattt tgcacgttca cgccgcgctc tggcgcaatt taatccgcat
     4801 gtctggtatc tttcgtatcc gtttggcgga tttaatgaca aagccgtgaa ggcagcaaac
     4861 gatgccggat ttcacctggc ggtgacaacc atgaaaggca aagtaaaacc gggggataat
     4921 ccgttgttac taaaacgact ttatatctta agaacggatt cgctggagac gatgtcgcgg
     4981 ctggtgagta accagccgca gggataacaa tcaagcaacc tgtaccggaa tcgctttcgc
     5041 ggtacgtttc atttcattgt cgccttcaaa ataggcgacg ttgggtcgcc aggtgcgagc
     5101 ttcttcatct ggcatggtaa cgaagctggc gatgatgaca atatcgccga cactggcgca
     5161 gtgggccgcc gcaccgttaa cagaaataat tctcgaaccg cgttctgccg cgatggcata
     5221 agtggagaaa cgcttgccgt tggtgacatt ccagatatca atggcttcgt tttcgagaat
     5281 accggctgcg tcaagaaaat cctggtcaat ggcgcaagaa ccttcatagt gcaggtccgc
     5341 atgagtcact ttcacgcggt ggagtttgcc ctgcagcatc gtgcgaatca taacttctac
     5401 ctttctaccc tgtcgttaac gaagcaggcg atgcctgctt tgaggaaatt ctcacgcagt
     5461 attgcccgat ttttgtttag tgtctactca tctgacggca tttacatcag cagtttgcgt
     5521 accgtgccga agcgtgccat gtaacggctg taaccaattg aaatttctct gaacgctgct
     5581 cggtaaacac taaagaggtg gctgacgatg gcacgccttg actggatact gccttcacgc
     5641 aagacaacac ggaaggcagg gaagatggat gcaccgagta ccacaccgca tgatgcagtg
     5701 tttaaacaat ttttaatgca cgcggagacg gctcgcgact ttctggagat acatttgcca
     5761 gtggaattac gagaactttg tgacctcaac acgcttcatt tagagtcggg gagtttcatt
     5821 gaagagagcc tgaaaggaca cagcacggac gtgctctatt ccgtgcaaat gcagggcaat
     5881 cccggttatc tgcatgttgt gattgaacac caaagcaagc cggataagaa aatggccttt
     5941 cgcatgatgc gttattctat tgctgccatg caccggcatc tggaggcgga tcacgataag
     6001 ctgccgctgg tggtgccgat actgttttat cagggcgagg ccacacctta tccgctctca
     6061 atgtgctggt ttgatatatt ttactcgccg gagctggcac gacgcgtcta taacagtcct
     6121 ttcccgctgg tggatatcac catcacaccg gatgacgaaa tcatgcaaca tcggcggatt
     6181 gcgattctcg aactactgca aaaacatatt cgccagcgcg acttaatgtt attgctggag
     6241 caactggtca cgctgattga cgaagggtac actagcggaa gtcagttagt tgccatgcaa
     6301 aactatatgc tgcaacgcgg tcatactgaa caagcggatt tgttttatgg tgtgctgaga
     6361 gacagggaaa cgggagggga gtctatgatg acgctggcgc agtggtttga agagaaaggg
     6421 attgagaagg ggattcagca gggaagacag gaggaaaggc aagaattcgc cctgcgtctt
     6481 ctaagtaaag gaatgtctcg ggaagacgtt gcagagatgg caaatttacc tcttgctgag
     6541 attgataagg taattaacct tatttaagtt acctgtgtta tgacagatga cgtggggtaa
     6601 attaataact ggcgccatca gccgtagcgc cagttaagta ttacgccagc tcgaccattt
     6661 tgttgtcgat caggcgagca tcgccaagcc aggcggctac cagaattact gcccgtttgc
     6721 tggtttcaga aacttccagc aatgtgtcgg catcgcgaat ctgaatatca tcggcgcgga
     6781 agcctttttc attcagttct tgccccgcaa tggtaataat ttcatcgaga tcccgttccc
     6841 cagcctgcaa tttgtcggca attgaactta aaactttgta cagaccgggc gcaattttgc
     6901 gttgttccgc cgtcagataa ccattacggg aactgagcgc aaggccatct ttggcgcgta
     6961 taatcgggac gccgacaatt tcaatatcga agcccatatc ggcaaccatt ttgcggatta
     7021 gcgccagttg ctgaaagtcc ttttcaccga agcaggcgat gtccggctgg accaggttga
     7081 acagcttgct gacaatagtc gaaacgccgc gaaaatgtcc cggacggctg gcaccttcca
     7141 gcatggtcga aaggccagga acgtcaacgt aagtgtgggt ttcagtaccg ttcgggtaga
     7201 tctcttttac cgaaggggcg aaaactaaat ccactttacg tttgtttagc ttttcgcagt
     7261 cctcttgcaa ggtccgtgga taacgagcca gatcttccgg gcggtcaaac tgcattgggt
     7321 taacgaaaat actgacgacg accacatcgg cgcgggcttt ggcttcgtcg accagcttca
     7381 tatggccatc gtgcagatta cccatagtag gcaccagcgc cacgcgcttg ccttccatac
     7441 gcaggcggcg aatttgctga cgcagcagcg gcagggtttc gataattaac acaacgtgac
     7501 tccttaatgg aaactgtgtt cttcgcccgg ataaacgccg gactccactt cagccatata
     7561 ctgccgcaca gccgcgcgga tgtcgcccgt ttcggcgagg aaatttttag cgaatttagg
     7621 aatgtgaccg ccggtaatgc cgaaggcgtc gtgcatcacg aggatctgcc cgtcagtgac
     7681 gttgcctgcg ccaatgccaa taaccgggat cgccagtgca tcggtaatac gttttgccag
     7741 ttcaaccggc acgcattcca gcaccagcag ctgtgcccca gcggcttcta aggctaatgc
     7801 atcgctgagc agttgatcgc ccgcttcatc gccgcgcccc tgaactttgt agccaccgaa
     7861 aatattcact gactgtgggg ttaaacctaa gtgaccacat acaggaacgg cacgttcggt
     7921 cagcattttt acggtttcga ccagccactc accgccctca attttgacca tattggcacc
     7981 ggcacgcata accgttgcgg cgttttcaaa ggcttgttcc ggcgtggcat acgccataaa
     8041 cggcagatca gccagcagca ggcagtttgg cgcgccgcga cgtacggcgg cagtgtggta
     8101 ggcgatatca gcaacggtca ccggcagggt ggagtcgtga ccctgaaccg tcatgcccag
     8161 cgagtcgcct accagcatga cgttaagtcc ttcatcagca aatagtttcg cgaagctgta
     8221 gtcgtaagcg gtgatggtcg cgaaacgctt tttatcctgc ttacatttct gaagtgaagc
     8281 aatggtggtt ggtttcataa cgtatcctga taaatgatgt cgtgttgcct ggcattttat
     8341 cagtcacatt ggtgggggca atgatttatc aattactgca ccgccagatg atggcggtgc
     8401 ggtggactta ttaagggtag gttacctgga aagtactggt ggctttaaac tcaccagctt
     8461 ctattggtat agttccatcc tgttgtagcg tggcctggaa atggagaggg tatgttatac
     8521 ctgtatcttg atccgggtaa actcctcctg ttgtatcgtc tttgcctctc gggtcgtaat
     8581 ctttataaac agaatttgaa tcattaggtt tcaatgtcat tagaggattt ttactagttg
     8641 ctaaaccttc tatgagtaca ccgactcctt ttgctgcgtc attacctgtg agagtattac
     8701 caaggagttg cccgttttca gtaccaacct ttgttgaaac taattttgtc tcaatattag
     8761 tcacacgaac gcagttttga agtgaaatat caaaaggaac cggtgtggca ccgtttctga
     8821 tttgttctgc actatattca cccattttta ccgtagaacc agtcacagaa gttccactaa
     8881 gcatagcggt aaaacaagtg ggattagtca acttgacatt gagcagatcg aaatcgattt
     8941 tgataaaata actatgctca tttatacctt ggcctgcgcc ataggcttta aatgaataca
     9001 gataatttga tgagctggat aatttgatct gttgagttac tgttggatca aagtttgtat
     9061 ctgtatagaa ttcaattgta agggtctgat aaataccacc tacagcccag tatttacggg
     9121 tgctattcgc attattgcaa ctctaatata acacgtcttc gcttggatta taccacgaaa
     9181 aatattgttc tgcagagtca ccaatgtata tcccaggtga actaacgtta gttacggtac
     9241 cgtaagctga ccacatgttt gaaattaata atgtgtaata caaaccagga actgatgtat
     9301 taaataattt atgggagcca tacattttac ctgagtcaac cattgcattt tcaatacgca
     9361 ttgcgctacc acttgtgcta tcacttctgt tgcaataaag aaaaccacca ccagaagcgg
     9421 tatctaactg actccactgt aattttacac ctgtaggtcc aagctgcgtc ggtttgtaga
     9481 aaactaaatt atttgtactt gttgcatatc tgaatatgac agtagcatcg ccattcggcc
     9541 ccgtttctct ccatttttga tctgacggac caacgccgta actattccca acaatatatc
     9601 ctacgttatc atcagtgcca gcactcgcgg t
//
LOCUS       DAZKHF020000175         9599 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2201S31241
            SAMN43267800-rid25106413.denovo.176, whole genome shotgun sequence.
ACCESSION   DAZKHF020000175 DAZKHF020000000
VERSION     DAZKHF020000175.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267800
            Sequence Read Archive: SRR30307263
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 9599)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9599)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 9599)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350581.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350581.2
            Genome Coverage       :: 151x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:21:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,652
            CDSs (total)                      :: 5,557
            Genes (coding)                    :: 5,320
            CDSs (with protein)               :: 5,320
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 237
            CDSs (without protein)            :: 237
            Pseudo Genes (ambiguous residues) :: 0 of 237
            Pseudo Genes (frameshifted)       :: 102 of 237
            Pseudo Genes (incomplete)         :: 130 of 237
            Pseudo Genes (internal stop)      :: 52 of 237
            Pseudo Genes (multiple problems)  :: 41 of 237
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9599
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267800-rid25106413.denovo.176"
                     /serotype="O 26:H11"
                     /isolate="2201S31241"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-01"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            8..178
                     /locus_tag="ACHOSC_RS17025"
                     /old_locus_tag="pgaptmp_003405"
     CDS             8..178
                     /locus_tag="ACHOSC_RS17025"
                     /old_locus_tag="pgaptmp_003405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004177212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4549290.1"
                     /translation="MVILLAIIACIYGLLVPFLISMKDTIAVTSGFALAFLTPPCIYA
                     IYKGLSFTKDKK"
     gene            175..1002
                     /locus_tag="ACHOSC_RS17030"
                     /old_locus_tag="pgaptmp_003406"
     CDS             175..1002
                     /locus_tag="ACHOSC_RS17030"
                     /old_locus_tag="pgaptmp_003406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH domain-containing protein"
                     /protein_id="HGU4549291.1"
                     /translation="MKKIIFALAIVLPTIGLVGCDRVEPGNVGIKVNKLGDDKGVGEV
                     VGVGRYWTGWNTEVYIFPTFKQMKTYDEPFSFQMSDGTTIGYHIGVAYKVDPSKVTTV
                     FQTYRKGVDDITATDLRQKIADALNRLASKMTTDKFIDGGKSELLDAALKDIQEEMTP
                     IGVQVMSLSYVGKPEYPPTVIDSINAKVTANQKTLQREQEVKQREAEANMLRAEAAGQ
                     ADAIRTKALAEADAIRLRGEALRQNPGVMELEAINKWNGTLPQYMTSGANTPFIQIK"
     gene            1043..1414
                     /locus_tag="ACHOSC_RS17035"
                     /old_locus_tag="pgaptmp_003407"
     CDS             1043..1414
                     /locus_tag="ACHOSC_RS17035"
                     /old_locus_tag="pgaptmp_003407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001695578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5406 family protein"
                     /protein_id="HGU4549292.1"
                     /translation="MNNQNTQPQIMNYDPNLTSCGRMAKQTVRLTFGLWEYRETFEVT
                     VGGNLTGLDVINCAIESLYATLPYEEVLDERTGKTDIMATINIGELICQDEDLSGELW
                     LAGMLISAEIISIEPATNIRL"
     gene            1446..1550
                     /locus_tag="ACHOSC_RS17040"
                     /old_locus_tag="pgaptmp_003408"
                     /pseudo
     CDS             1446..1550
                     /locus_tag="ACHOSC_RS17040"
                     /old_locus_tag="pgaptmp_003408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000457709.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4222 domain-containing protein"
     gene            1606..1860
                     /gene="xisR"
                     /locus_tag="ACHOSC_RS17045"
                     /old_locus_tag="pgaptmp_003409"
     CDS             1606..1860
                     /gene="xisR"
                     /locus_tag="ACHOSC_RS17045"
                     /old_locus_tag="pgaptmp_003409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excisionase family protein"
                     /protein_id="HGU4549293.1"
                     /translation="MQTIIYQITPSKWCTERVLIASTGLKPGTIERARRKSWMQGKEY
                     RHYAVEGDPGHYSECLYNIEEIMRWIENQKQPGAKNASSG"
     gene            1894..3180
                     /locus_tag="ACHOSC_RS17050"
                     /old_locus_tag="pgaptmp_003410"
     CDS             1894..3180
                     /locus_tag="ACHOSC_RS17050"
                     /old_locus_tag="pgaptmp_003410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001537108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HGU4549294.1"
                     /translation="MSNASYPTGVENHGGSLRIWFHYNGKRVRENLGVPDTAKNRKIA
                     GELRTSVCFAIRMGSFDYAAQFPNSPNLKHFGLGKREITVKALSEKWLDLKKIEIGSN
                     AFSRYQSVVRNMLPRIGEKRLASSVTKEDLLFIRKDLLTGYHNLSNGKTTPIKGRSVV
                     TVNYYMTTIAGMFQFAADNGYIVSNPFNGLTPLKRSRTEPDPLTRDEFIRFIDACHHQ
                     QTKNLWSLAVYTGIRHGELISLAWEDIDLKAKTMTIRRNYTKLGEFTLPKTEAGTDRV
                     IHLVQPAVDALKSQAEMTRLGPQYQIDVKLREFGRTARHECTFVFNPQLVKKCQQVGH
                     HYKADSIRDSWASALRRAGLRHRKAYQSRHTYASWALSAGANPSFIANQMGHANAQMV
                     FNVYGAWMKDNNIGQIELLNKQLTESVPYMPHRARL"
     gene            3164..3961
                     /gene="yecE"
                     /locus_tag="ACHOSC_RS17055"
                     /old_locus_tag="pgaptmp_003411"
     CDS             3164..3961
                     /gene="yecE"
                     /locus_tag="ACHOSC_RS17055"
                     /old_locus_tag="pgaptmp_003411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000019900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF72 domain-containing protein"
                     /protein_id="HGU4549295.1"
                     /translation="MEPDSEIISLNISCFSGSCRHINCVEGNTTLYALPKPEVVLRWR
                     EQTTDDFRFCFKFPATISHQAALRHCDDLVTEFLTRMSPLAPRIGQYWLQLPATFGPR
                     ELPALWHFLDSLPGEFNYGVEVRHPQFFAKGEEEQTLNRGLHQRGVNRVILDSRPVHA
                     ACPHSEAIRDAQRKKPKVPVHAVLTATNPLIRFIGSDDMTQNRELFQVWLQKLAQWHQ
                     TTTPYLFLHTPDIAQAPELVHTLWEDLRKTLPEIGAVPAIPQQSSLF"
     gene            4014..4409
                     /gene="yecN"
                     /locus_tag="ACHOSC_RS17060"
                     /old_locus_tag="pgaptmp_003412"
     CDS             4014..4409
                     /gene="yecN"
                     /locus_tag="ACHOSC_RS17060"
                     /old_locus_tag="pgaptmp_003412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310606.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MAPEG family protein"
                     /protein_id="HGU4549296.1"
                     /translation="MVSALYAVLSALLLMKFSFDVVRLRLQYRVAYGDGGFSELQSAI
                     RIHGNAVEYIPIAIVLMLFMEMNGAETWMVHICGIVLLAGRLMHYYGFHHRLFRWRRS
                     GMSATWCALLLMVLANLWYMPWELVFSLR"
     gene            4450..5193
                     /gene="cmoA"
                     /locus_tag="ACHOSC_RS17065"
                     /old_locus_tag="pgaptmp_003413"
     CDS             4450..5193
                     /gene="cmoA"
                     /locus_tag="ACHOSC_RS17065"
                     /old_locus_tag="pgaptmp_003413"
                     /EC_number="2.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxy-S-adenosyl-L-methionine synthase CmoA"
                     /protein_id="HGU4549297.1"
                     /translation="MSHRDTLFSAPIARLGDWTFDERVAEVFPDMIQRSVPGYSNIIS
                     MIGMLAERFVQPGTQVYDLGCSLGAATLSVRRNIHHDNCKIIAIDNSPAMIERCRRHI
                     DAYKAPTPVDVIEGDIRDIAIENASMVVLNFTLQFLEPSERQALLDKIYQGLNPGGAL
                     VLSEKFSFEDAKVGELLFNMHHDFKRANGYSELEISQKRSMLENVMLTDSVETHKARL
                     HKAGFEHSELWFQCFNFGSLVALKAEDAA"
     gene            5190..6161
                     /gene="cmoB"
                     /locus_tag="ACHOSC_RS17070"
                     /old_locus_tag="pgaptmp_003414"
     CDS             5190..6161
                     /gene="cmoB"
                     /locus_tag="ACHOSC_RS17070"
                     /old_locus_tag="pgaptmp_003414"
                     /EC_number="2.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016765 - transferase activity,
                     transferring alkyl or aryl (other than methyl) groups
                     [Evidence IEA];
                     GO_process: GO:0002098 - tRNA wobble uridine modification
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 5-methoxyuridine(34)/uridine 5-oxyacetic
                     acid(34) synthase CmoB"
                     /protein_id="HGU4549298.1"
                     /translation="MIDFGNFYSLIAKNHLSHWLETLPAQIANWQREQQHGLFKQWSN
                     AVEFLPEIKPYRLDLLHSVTAESEEPLSTGQIKRIETLMRNLMPWRKGPFSLYGVNID
                     TEWRSDWKWDRVLPHLSDLTGRTILDVGCGSGYHMWRMIGAGAHLAVGIDPTQLFLCQ
                     FEAVRKLLGNDQRAHLLPLGIEQLPALKAFDTVFSMGVLYHRRSPLEHLWQLKDQLVN
                     EGELVLETLVIDGDENTVLVPGDRYAQMRNVYFIPSALALKNWLKKCGFVDIRVVDVC
                     VTTTEEQRRTEWMVTESLSDFLDPHDPSKTVEGYPAPKRAVLIARKP"
     gene            complement(6326..8755)
                     /gene="torZ"
                     /locus_tag="ACHOSC_RS17075"
                     /old_locus_tag="pgaptmp_003415"
     CDS             complement(6326..8755)
                     /gene="torZ"
                     /locus_tag="ACHOSC_RS17075"
                     /old_locus_tag="pgaptmp_003415"
                     /EC_number="1.7.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310609.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trimethylamine N-oxide reductase TorZ"
                     /protein_id="HGU4549299.1"
                     /translation="MTLTRREFIKHSGIAAGALVVTSAAPLPAWAEEKGGKILTAGRW
                     GAMNVEVKDGKIVSSTGALAKTIPNSLQSTAADQVHTTARIQHPMVRKSYLDNPLQPA
                     KGRGEDTYVQVSWEQALKLIHEQHDRIRKANGPSAIFAGSYGWRSSGVLHKAQTLLQR
                     YMNLAGGYSGHSGDYSTGAAQVIMPHVVGSVEVYEQQTSWPLILENSQAVVLWGMNPL
                     NTLKIAWSSTDEQGLEYFHQLKKSGKPVIAIDPIRSETIEFFGDNATWIAPNMGTDVA
                     LMLGIAHTLMTQGKHDKVFLEKYTTGYPQFEEYLTGKSDNTPKSAAWAAEITGVPEAQ
                     IVKLAELMAANRTMLMAGWGIQRQQYGEQKHWMLVTLAAMLGQIGTPGGGFGFSYHYS
                     NGGNPTRVGGVLPEMSAAIAGQASEAADDGGMTAIPVARIVDALENPGGKYQHNGKEQ
                     TYPNIKMIWWAGGGNFTHHQDTNRLIKAWQKPEMIVVSECYWTAAAKHADIVLPITTS
                     FERNDLTMTGDYSNQHIVPMKQAVAPQFEARNDFDVFADLAELLKPGGKEIYTEGKDE
                     MAWLKFFYDAAQKGARAQRVTMPMFNAFWQQNKLIEMRRSEKNEQYVRYADFRADPVK
                     NALGTPSGKIEIYSKTLEKFGYKDCPAHPTWLAPDEWKGTADEKQLQLLTAHPAHRLH
                     SQLNYAELRKKYAVADREPITIHTEDAARFGIANGDLVRVWNKRGQILTGAVVTDGIK
                     KGVVCVHEGAWPDLENGLCKNGSANVLTADIPSSQLANACAGNSALVYIEKYTGNAPK
                     LTAFDKPAVQA"
     gene            complement(8780..>9599)
                     /locus_tag="ACHOSC_RS17080"
                     /old_locus_tag="pgaptmp_003416"
     CDS             complement(8780..>9599)
                     /locus_tag="ACHOSC_RS17080"
                     /old_locus_tag="pgaptmp_003416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="NapC/NirT family cytochrome c"
                     /protein_id="HGU4549300.1"
                     /translation="FVSGKIDSDDKFETHRQEMAETVWKELKATDSATCRSCHSFDAM
                     DIASQSESAQKMHNKAQKGGETCIDCHKGIAHFPPEIKMDDNAAHELESQAATSVTNG
                     AHIYPFKTSRIGELATVNPGTDLTVVDASGKQPIVLLQGYQMQGSENTLYLAAGQRLA
                     LATLSEEGIKALTVNGEWQADEYGNQWRQASLQGALTDPALADRKPLWQYAEKLDDTY
                     CAGCHAPIAADHYTVNAWPSIAKGMGARTSMSENELDILTRYFQYNAKDITEKQ"
BASE COUNT         2381 a         2426 c         2317 g         2475 t
ORIGIN      
        1 tttcacaata gtaattttac tggcaattat cgcatgcatt tacggattac tcgttccgtt
       61 ccttatatcc atgaaggata cgatagcagt tacttctggc tttgcactgg cgtttctgac
      121 cccgccctgc atttatgcca tttacaaggg tctttctttc actaaggata aaaaatgaaa
      181 aaaattattt ttgctttagc cattgttctg ccgaccattg gccttgtcgg ctgcgatcgc
      241 gttgaacccg gtaatgttgg catcaaagta aataaactgg gcgacgataa aggcgtcggt
      301 gaggtggtcg gtgttggtcg ctactggact ggctggaata ctgaagttta catcttccca
      361 accttcaaac aaatgaagac ctacgatgag ccgttcagct tccagatgag tgacggcaca
      421 accatcggct atcacatcgg tgttgcctac aaagttgatc catccaaagt taccacggtg
      481 tttcagacct accgcaaagg cgtggatgac attaccgcca ctgacctgcg ccagaagatc
      541 gccgacgcac tcaatcgact ggccagcaaa atgaccaccg acaaatttat cgacggcggc
      601 aagtctgaac tactggatgc cgcacttaaa gacattcagg aagagatgac ccccatcggc
      661 gttcaggtaa tgagcctctc ttatgtaggt aaaccggaat acccgccaac cgttatcgac
      721 agcattaacg ccaaagtcac ggcaaaccag aaaaccctgc agcgcgaaca ggaagtcaag
      781 caacgtgaag cggaggccaa catgctgcgt gcagaagctg ccggacaggc tgatgccatt
      841 cgaacaaaag ccctggcaga agccgatgcc attcgtttac gcggcgaagc tctgcgccag
      901 aaccctggcg tcatggagct ggaagccatc aacaagtgga acggtacact gccgcaatac
      961 atgaccagtg gtgccaatac accatttatc cagattaaat aacttacacg cccggcaaac
     1021 cgccgggtta atggaaaatc agatgaacaa ccagaatact caaccgcaaa taatgaacta
     1081 tgacccgaat ctgacgtcgt gcggacgcat ggcaaaacaa accgttcgat taactttcgg
     1141 actatgggaa taccgcgaaa cattcgaagt tactgtcggc ggcaatctga ccggactgga
     1201 tgttatcaat tgcgctattg aaagcctgta cgcaacgctg ccttatgaag aagtcctgga
     1261 tgagcgcaca gggaaaacgg atatcatggc caccattaat attggcgaac tgatatgcca
     1321 ggatgaagac ctgtccgggg aactctggct tgccgggatg cttatctcag cagaaattat
     1381 cagcattgaa cccgctacaa acatacggct ctgaagttct cactattcag agagcaggag
     1441 aaaaaatgtt cgctctgatt aatcagggac aactgtatac cgacagtgcc ggttacccgg
     1501 taaaaattgt tcgctgcata aacaacaccg tgttgtacag aagatgtgaa gcgaatgttt
     1561 gaagtgtggt gccactgccg tggatgaaag ttttatgagg ttggcatgca gacaatcatc
     1621 tatcagataa cccccagcaa atggtgtacg gagagagtcc tcattgcatc aacagggcta
     1681 aagcctggca ccatcgagcg agccagaaga aagtcatgga tgcagggaaa agaataccgc
     1741 cattacgctg tagaaggtga tccggggcat tacagtgaat gcctgtacaa catcgaagaa
     1801 attatgcgat ggatcgaaaa ccagaaacaa ccaggtgcca aaaatgcaag ttccggttaa
     1861 cctgttaatg ctcctggacg tctgggaggt tttatgagta acgcatcata cccgacaggc
     1921 gttgaaaacc atggaggatc actccgtata tggtttcact ataatggcaa acgtgtcaga
     1981 gaaaacctcg gtgttcctga caccgccaaa aaccggaaga tcgctggtga gcttcgcact
     2041 tctgtttgtt ttgcaatcag aatggggagt ttcgactacg ccgcgcagtt ccctaattcc
     2101 cctaacctga aacactttgg tctgggaaaa agagagataa ccgttaaggc actttcggaa
     2161 aaatggttgg accttaagaa aattgagata ggaagtaatg cattcagtcg gtatcaatcc
     2221 gtggtgagaa acatgcttcc tcgcataggg gaaaaacgtc ttgcttcgtc ggtaacaaag
     2281 gaagatttac tgtttatcag gaaagattta ttgaccgggt atcataatct ttctaacgga
     2341 aaaacaacgc cgattaaagg gaggtcagta gttacggtta attactacat gacgacaatt
     2401 gcaggaatgt ttcaatttgc ggctgataac ggctatatcg tgtcaaaccc atttaacggc
     2461 ctgacaccat taaagagatc cagaacagaa ccagatccgc tcacacgtga cgaatttatt
     2521 cgttttattg atgcctgtca ccatcaacaa acaaaaaacc tgtggtcctt agcagtatac
     2581 acaggcattc gtcacggtga gctaatatct ctcgcctggg aggatattga tttaaaagct
     2641 aaaacaatga ctatccgtcg taattataca aaactcgggg agttcactct accaaaaaca
     2701 gaagcgggaa ctgatcgtgt tattcatctt gttcaaccag ctgttgatgc cctgaaaagc
     2761 caggctgaaa tgacaagact tggccctcag tatcaaattg acgtcaagct tcgggagttc
     2821 ggtcgcaccg cacgccatga atgcacgttt gtttttaatc cgcaactggt aaaaaaatgc
     2881 cagcaagtcg gtcaccacta taaagcagat tccatcagag attcctgggc atctgcatta
     2941 aggcgagcag gactgcggca cagaaaagcc tatcagtcta ggcatactta tgccagctgg
     3001 gcattatcgg caggagcgaa tccaagcttc atagcaaacc aaatgggcca tgcaaatgca
     3061 caaatggtat tcaacgttta cggagcatgg atgaaagata acaatatcgg gcaaatagaa
     3121 ctactcaata agcagttgac ggagagtgtc ccatacatgc cccatagagc cagactctga
     3181 aataatatct ttaaatatca gttgttttag tggtagttgc cgtcacatta actgcgtgga
     3241 gggcaacacc acgctttacg ccctgccgaa acccgaggtt gtcctgcgct ggcgtgaaca
     3301 gaccacagat gacttccgct tctgttttaa gtttccggcg accatttcgc atcaggcagc
     3361 attacgacat tgcgatgatt tagtgactga atttttgacc cgcatgtcac cgttggctcc
     3421 gcgcattggg caatactggc tgcaactgcc tgccacattc ggcccacggg agctgcctgc
     3481 gctttggcat tttctcgatt ctctccctgg cgaatttaat tatggtgtgg aagtccgcca
     3541 tccacagttt ttcgccaaag gggaagagga acaaacgctt aatcgcggtt tacatcagcg
     3601 cggcgttaat cgggtgattt tagacagccg cccggttcat gcagcatgtc cacacagtga
     3661 agctattcgc gacgctcaac gaaaaaaacc taaagttccg gtacatgctg tactgacggc
     3721 gacaaatccg ctgatccgtt ttatcggtag tgatgatatg acgcaaaacc gggaattatt
     3781 tcaggtctgg ttacagaaat tagcgcagtg gcatcagacc actacgcctt atcttttttt
     3841 acatacgcca gacattgccc aggccccgga actggtacat accctgtggg aagacttacg
     3901 taaaacgctt ccagagatcg gagcagttcc ggctattcca cagcaatctt ctcttttctg
     3961 aatttgccac ctatcataga caggtgccat cggccatttt aaagggagtt tgtatggtaa
     4021 gcgcgctata tgccgtttta agtgcgttgt tattaatgaa gttctctttt gatgtcgttc
     4081 gcttgcgttt gcagtaccgc gttgcctatg gtgacggtgg ttttagcgaa ctgcaaagcg
     4141 ctattcgcat tcatggtaac gcggtggaat atattcccat cgcgattgtg ctgatgctgt
     4201 ttatggaaat gaatggcgca gaaacctgga tggtgcatat ttgcggcatc gttttgcttg
     4261 ctggtcgtct gatgcattat tacggttttc atcaccgtct gtttcgctgg cgacgttccg
     4321 gcatgagcgc cacctggtgt gcgctgttgc tgatggtgct ggcgaatctt tggtatatgc
     4381 cctgggagtt ggttttctcc ctgcgttagc gcacaatacg ccactttctt tttcccggat
     4441 ttttacgtta tgtctcaccg cgacacgcta ttttctgccc ctatcgccag actgggcgac
     4501 tggacctttg atgaacgggt agctgaagtc ttcccggata tgatccagcg ttccgttccc
     4561 ggctattcca atattatttc catgattggt atgttagccg agcgcttcgt tcaacctggt
     4621 acgcaggttt acgatctggg ttgttctctg ggcgcggcga cgctctcggt gcgtcgcaac
     4681 attcatcatg ataattgcaa aattattgcc atcgacaact ccccggcgat gattgaacgc
     4741 tgccgtcgtc atattgacgc ctataaagcc cctacgccag tagacgtcat tgaaggtgat
     4801 attcgcgata tcgccattga aaacgcatcg atggtggtgc tgaattttac cctgcaattc
     4861 ctggaacctt ccgagcgcca ggcattactg gataaaattt atcaaggact gaacccgggc
     4921 ggcgcgctgg tgctttcgga aaaattcagt ttcgaagatg ccaaagttgg tgaactgttg
     4981 ttcaacatgc accacgactt taaacgtgcc aacggttaca gcgaactgga gatcagccag
     5041 aagcgcagca tgctggaaaa cgtgatgctg accgattccg tggaaaccca taaagcacgc
     5101 ctgcataaag ccggttttga gcatagcgag ctgtggttcc agtgctttaa ctttggttca
     5161 ctggtggcat taaaagcaga ggacgctgca tgatcgactt tggtaacttt tattctctga
     5221 ttgccaaaaa tcatctttca cactggctcg aaacgctgcc cgcgcagatt gctaactggc
     5281 agcgcgagca gcagcacggg ctgtttaagc agtggtccaa cgcggtggaa tttctgcctg
     5341 aaattaaacc gtatcgtctg gatttattgc atagcgtaac cgccgaaagc gaagagccac
     5401 tgagcaccgg gcaaattaaa cgcattgaaa cgcttatgcg gaacctgatg ccatggcgca
     5461 aagggccgtt ctcactgtat ggcgtcaaca tcgataccga atggcgttcc gactggaaat
     5521 gggatcgtgt tctgccccat ctttctgatt taaccggacg caccattctg gatgtcggct
     5581 gcggcagcgg ttatcacatg tggcgcatga ttggcgcagg cgcgcacctc gcggtgggga
     5641 tcgaccctac gcaactgttt ctgtgccagt ttgaagcggt gcgtaaacta ctgggtaacg
     5701 atcagcgcgc acatttgtta ccgttaggta ttgaacagct tccggcactg aaagcctttg
     5761 ataccgtctt ttcgatgggc gtgctttacc atcgccgttc gccgctggag catctctggc
     5821 agttgaaaga tcaactggtg aatgagggtg aactggtgct ggaaacgctg gttattgatg
     5881 gcgatgagaa cacggtgctg gtaccaggcg atcgttacgc gcaaatgcgt aatgtctatt
     5941 tcatcccttc cgcgctggcg ctgaaaaact ggctgaagaa gtgtggtttt gtagatatcc
     6001 gcgttgtgga tgtgtgcgtt accactactg aagagcagcg acgcactgaa tggatggtta
     6061 ccgaatctct gtccgatttc ctcgacccac atgatccgag taagacagtt gaaggttatc
     6121 ctgcgcctaa acgcgcggtg ctgattgcgc gcaagccgta aaggtctggc attactgccg
     6181 gatgcggctt gaatgcctta tccggcctac aaacccttgc taattcaatc tattgcaggg
     6241 actatgtagg cctgataagc atagcgcatc aggcagtttt acgtttgcat aacctcagcg
     6301 cccgtttccg ggcgctattc acttcttacg cctgaacagc aggcttatca aatgccgtta
     6361 acttcggtgc gttacccgta tatttttcga tatacaccag cgcagagtta ccggcacagg
     6421 catttgccag ctgcgagctg gggatatccg ccgttaacac gttcgcactg ccgtttttac
     6481 acaagccgtt ttccagatct ggccatgcac cttcatgcac gcataccacg ccttttttga
     6541 tcccgtcagt caccaccgcg cctgtcaaaa tctgaccacg tttgttccag acacgcacca
     6601 gatcgccatt cgcaatacca aatcgagcag catcttcggt gtgaatagtg atcggttcac
     6661 gatcggcaac cgcatatttt ttacgcagtt ccgcatagtt aagctgacta tgtaaacggt
     6721 gtgccggatg tgcggtcaga agctgcaact gcttctcgtc ggcggtaccc ttccactcat
     6781 caggcgcaag ccaggttgga tgtgccgggc aatccttata gccaaatttt tccagcgttt
     6841 tggagtaaat ctcaattttg ccgcttggcg tacccagcgc atttttcacc ggatcggcgc
     6901 ggaaatcagc ataacgaacg tactgttcgt tcttctcgct gcggcgcatt tcgatcagtt
     6961 tattttgctg ccagaaggca ttaaacatcg gcatagtcac gcgttgcgca cgagcacctt
     7021 tctgagcggc atcatagaaa aatttcagcc acgccatttc atctttacct tcggtataga
     7081 tctcttttcc gccaggtttc agtaattcag caagatcggc aaatacgtca aaatcgttac
     7141 gcgcttcaaa ttgcggagca acagcctgct tcatcggcac aatatgctgg ttgctgtaat
     7201 caccggtcat cgtcaagtca ttgcgctcaa acgatgtggt gatcggtaat acgatatctg
     7261 catgtttagc agctgcggtc cagtagcatt cagaaacgac gatcatctcc ggtttctgcc
     7321 atgctttgat cagacgatta gtatcctggt gatgggtaaa gttaccgcca cccgcccacc
     7381 agatcatttt gatattcgga tacgtttgtt ctttaccgtt atgctgatat ttgccaccag
     7441 gattttccag cgcatcgacg atacgggcaa caggaatcgc cgtcattccg ccatcatcag
     7501 cggcctcgct ggcctgtccg gcaatcgccg cagacatttc cggcagcacg ccgccaacac
     7561 gcgtcggatt gccaccgttg gagtaatgat aagagaaacc aaaaccgccg cccggtgtac
     7621 caatctgccc taacatcgcc gccagcgtca ccagcatcca gtgtttctgt tcaccatatt
     7681 gctggcgctg aattccccag ccagccatca gcattgtgcg attcgccgcc atcagttcag
     7741 cgagtttgac tatctgcgct tccggtacgc cagtaatttc cgccgcccag gccgcgctct
     7801 tcggagtatt atcgctctta cctgtcagat actcttcaaa ctgcggatac ccggtggtgt
     7861 atttttcaag gaacacttta tcgtgtttac cttgtgtcat cagggtatgc gcaatcccta
     7921 acatcagtgc cacgtcggtg cccatattcg gcgcgatcca ggtggcatta tcaccaaaga
     7981 actcgatggt ttcggagcgg ataggatcaa tggcaatcac tggtttgcca gattttttca
     8041 gctgatggaa gtattccagc ccttgctcat cggtactgct ccaggcaatt tttaaggtat
     8101 tcagcgggtt catcccccac agcaccacag cctggctgtt ttccagaatc agcggccagg
     8161 aggtctgctg ttcatacacc tctacagaac cgaccacatg cggcatgatc acctgtgctg
     8221 ccccggttga ataatcgccg ctatgcccgg aataaccgcc cgccaggttc atataacgtt
     8281 gcagtaaggt ttgcgcttta tgcaacacgc cagaagagcg ccagccgtat gatccggcaa
     8341 aaatagcaga tggtccgtta gctttgcgaa tgcgatcgtg ttgctcatga atcagtttta
     8401 atgcttgctc ccagctcacc tgtacataag tatcttcgcc acgacctttc gccggttgca
     8461 gtggattatc gagatagctt tttcttacca tcggatgctg aatacgtgcc gtggtgtgta
     8521 cctgatccgc cgccgtagac tgtaaggagt tcggtatggt tttcgccagc gcgcctgttg
     8581 aagaaacaat cttgccgtcc ttcacttcta cgttcatcgc tccccaacgt cccgcggtga
     8641 ggattttacc gcccttctct tctgcccatg cggggagcgg tgctgccgat gtcaccacca
     8701 gcgctccagc ggcaataccg ctgtgtttaa taaattcacg tcttgttaat gtcataactt
     8761 cctccctgat caacgaggat cactgtttct cggtaatatc tttggcgttg tactggaaat
     8821 accgcgttaa aatgtccagt tcgttttcgc tcatgctggt tcgcgcaccc attcctttgg
     8881 caatggacgg ccatgcattg acggtgtaat ggtcagcggc aataggcgca tggcaaccag
     8941 cgcaataggt atcgtcaagt ttttcagcgt attgccatag cggtttacgg tccgctaatg
     9001 cgggatcggt aagcgcaccc tgtaaagacg cctgacgcca ttgattgccg tattcgtcag
     9061 cctgccattc cccgtttacc gtgagcgcct tgataccttc ttcacttaat gtggctagcg
     9121 ccagccgttg acctgccgcc aggtagagcg tgttttcact gccctgcatt tgataaccct
     9181 gcaacagaac gatcggctgt ttgccactgg catcaacgac ggtgagatcg gtaccaggat
     9241 tcacggtagc cagctcgcct atgcgagaag ttttgaaagg ataaatatgt gcgccattag
     9301 tcactgaagt agcggcctga ctttccagct catgcgccgc gttgtcatcc atttttattt
     9361 ctggcggaaa atgggcaatg cctttatgac aatcgataca ggtttcgccg cccttttgtg
     9421 ctttgttatg cattttctgc gcagattcac tttgcgaggc aatatccatg gcatcaaaag
     9481 aatggcaact acggcacgtt gcagagtcag ttgcttttaa ttctttccat actgtttcgg
     9541 ccatttcctg gcgatgagtt tcgaacttat cgtcactgtc tattttgccg ctaacaaat
//
LOCUS       DAZKHF020000176         9554 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2201S31241
            SAMN43267800-rid25106413.denovo.177, whole genome shotgun sequence.
ACCESSION   DAZKHF020000176 DAZKHF020000000
VERSION     DAZKHF020000176.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267800
            Sequence Read Archive: SRR30307263
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 9554)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9554)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 9554)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350581.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350581.2
            Genome Coverage       :: 151x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:21:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,652
            CDSs (total)                      :: 5,557
            Genes (coding)                    :: 5,320
            CDSs (with protein)               :: 5,320
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 237
            CDSs (without protein)            :: 237
            Pseudo Genes (ambiguous residues) :: 0 of 237
            Pseudo Genes (frameshifted)       :: 102 of 237
            Pseudo Genes (incomplete)         :: 130 of 237
            Pseudo Genes (internal stop)      :: 52 of 237
            Pseudo Genes (multiple problems)  :: 41 of 237
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9554
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267800-rid25106413.denovo.177"
                     /serotype="O 26:H11"
                     /isolate="2201S31241"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-01"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            complement(46..258)
                     /gene="cspA"
                     /locus_tag="ACHOSC_RS17085"
                     /old_locus_tag="pgaptmp_003417"
     CDS             complement(46..258)
                     /gene="cspA"
                     /locus_tag="ACHOSC_RS17085"
                     /old_locus_tag="pgaptmp_003417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA chaperone/antiterminator CspA"
                     /protein_id="HGU4549301.1"
                     /translation="MSGKMTGIVKWFNADKGFGFITPDDGSKDVFVHFSAIQNDGYKS
                     LDEGQKVSFTIESGAKGPAAGNVTSL"
     gene            complement(539..829)
                     /locus_tag="ACHOSC_RS17090"
                     /old_locus_tag="pgaptmp_003418"
     CDS             complement(539..829)
                     /locus_tag="ACHOSC_RS17090"
                     /old_locus_tag="pgaptmp_003418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator"
                     /protein_id="HGU4549302.1"
                     /translation="MEYKDPMHELLSSLEQIVFKDETQKITLTHRTTSCTEIEQLRKG
                     TGLKIDDFARVLGVSVAMVKEWESRRVKPSSAELKLMRLIQANPALSKQLME"
     gene            1263..1973
                     /locus_tag="ACHOSC_RS17095"
                     /old_locus_tag="pgaptmp_003419"
     CDS             1263..1973
                     /locus_tag="ACHOSC_RS17095"
                     /old_locus_tag="pgaptmp_003419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418010.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3053 domain-containing protein"
                     /protein_id="HGU4549303.1"
                     /translation="MATGKSCSRWFAPLAALLMVVSLSGCFDKEGDQRKAFIDFLQNT
                     VMRSGERLPTLTADQKKQFGPFVSDYAILYGYSQQVNQAMDSGLRPVVDSVNAIRVPQ
                     DYVTQSGPLREMNGSLGVLAQQLQNAKLQADAAHSALKQTDDLKPVFDQAFTKVVTTP
                     ADALQPLIPAAQTFTQQLVMVGDYIAQQGTQVSFVANGIQFPTSQQASEYNKLIAPLP
                     AQHQAFNQAWTTAVTATQ"
     gene            complement(2023..2997)
                     /gene="ghrB"
                     /locus_tag="ACHOSC_RS17100"
                     /old_locus_tag="pgaptmp_003420"
     CDS             complement(2023..2997)
                     /gene="ghrB"
                     /locus_tag="ACHOSC_RS17100"
                     /old_locus_tag="pgaptmp_003420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312465.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glyoxylate/hydroxypyruvate reductase GhrB"
                     /protein_id="HGU4549304.1"
                     /translation="MKPSVILYKALPDDLLQRLQEHFTVHQVANLSPQTVEQNAAIFA
                     EAEGLLGSNENVDAALLEKMPKLRATSTISVGYDNFDVDALTARKILLMHTPTVLTET
                     VADTLMALVLSTARRVVEVAERVKAGEWTASIGPDWYGTDVHHKTLGIVGMGRIGMAL
                     AQRAHFGFNMPILYNARRHHKEAEERFNARYCDLDTLLQESDFVCLILPLTDETHHLF
                     GAEQFAKMKSSAIFINAGRGPVVDENALIAALQKGEIHAAGLDVFEQEPLSVDSPLLS
                     MANVVAVPHIGSATHETRYGMAACAVDNLIDALQGKVEKNCVNPHVAD"
     gene            complement(3101..3760)
                     /locus_tag="ACHOSC_RS17105"
                     /old_locus_tag="pgaptmp_003421"
     CDS             complement(3101..3760)
                     /locus_tag="ACHOSC_RS17105"
                     /old_locus_tag="pgaptmp_003421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family lipoprotein"
                     /protein_id="HGU4549305.1"
                     /translation="MKKRVYLIAAVVSGALAVSGCTTNPYTGEREAGKSAIGAGLGSL
                     VGAGIGALSSSKKDRGKGALIGAAAGAALGGGVGYYMDVQEAKLRDKMRGTGVSVTRS
                     GDNIILNMPNNVTFDSSSATLKPAGANTLTGVAMVLKEYPKTAVNVIGYTDSTGGHDL
                     NMRLSQQRADSVASALITQGVDASRIRTQGLGPANPIASNSTAEGKAQNRRVEITLSP
                     L"
     gene            3913..6246
                     /gene="bisC"
                     /locus_tag="ACHOSC_RS17110"
                     /old_locus_tag="pgaptmp_003422"
     CDS             3913..6246
                     /gene="bisC"
                     /locus_tag="ACHOSC_RS17110"
                     /old_locus_tag="pgaptmp_003422"
                     /EC_number="1.8.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418007.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin sulfoxide reductase"
                     /protein_id="HGU4549306.1"
                     /translation="MANSSSRYSVLTAAHWGPMLVETDGETVFSSHGALATGMENSLQ
                     SAVRDQVHSNTRVRFPMVRKGFLASPENPQGIRGQDEFVRVSWDEALDLIHQQHKRIR
                     EAYGPASIFAGSYGWRSNGVLHKASTLLQRYMALAGGYTGHLGDYSTGAAQAIMPYVV
                     GGSEVYQQQTSWPLVLEHSDVVVLWSANPLNTLKIAWNASDEQGLSYFSALRDSGKKL
                     ICIDPMRSETVDFFGDKMEWVAPHMGTDVALMLGIAHTLVENGWHDEAFLTRCTTGYA
                     VFASYLLGESDGIAKNAEWAAEICGVNAAKIRELAALFHQNTTMLMAGWGMQRQQFGE
                     QKHWMIVTLAAMLGQIGTPGGGFGLSYHFANGGNPTRRSAVLSSMQGSLPGGCDAVDK
                     IPVARIVEALENPGGAYQHNGMNRHFPDIRFIWWAGGANFTHHQDTNRLIRAWQKPEL
                     VVISECFWTAAAKHADIVLPATTSFERNDLTMTGDYSNQHLVPMKQVVPPRYEARNDF
                     DVFAELSERWEKGGYARFTEGKSQLQWLETFYNVARQRGASQQVELPPFAEFWQANQL
                     IEMPENPDSERFIRFADFCRDPLAHPLKTASGKIEIFSQRIADYGYPDCPGHPMWLEP
                     DEWQGNAEPEQLQVLSAHPAHRLHSQLNYSSLRELYAVANREPVTIHPDDAQARGITE
                     GDMVRVWNSRGQILAGAVISEGIKPGVICIHEGAWPDLDLTADGICKNGAVNVLTKDL
                     PSSRLGNGCAGNTALAWLEKYNGPELTLTAFEPPASS"
     gene            complement(6215..6655)
                     /locus_tag="ACHOSC_RS17115"
                     /old_locus_tag="pgaptmp_003423"
     CDS             complement(6215..6655)
                     /locus_tag="ACHOSC_RS17115"
                     /old_locus_tag="pgaptmp_003423"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
                     /protein_id="HGU4549307.1"
                     /translation="MIREAQRSELPAILELWLESTTWGHPFIKANYWRDCIPLVRDAY
                     LANAQNWVWEEDGKLLGFVSIMEGRFLAAMFVAPKAVRRGIGKALMQYVQQRYPHLML
                     EVYQKNQPAIDFYRAQGFHIVDCAWQDETQLPTWIMSWPVVQTL"
     gene            complement(6652..7215)
                     /gene="tag"
                     /locus_tag="ACHOSC_RS17120"
                     /old_locus_tag="pgaptmp_003424"
     CDS             complement(6652..7215)
                     /gene="tag"
                     /locus_tag="ACHOSC_RS17120"
                     /old_locus_tag="pgaptmp_003424"
                     /EC_number="3.2.2.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase I"
                     /protein_id="HGU4549308.1"
                     /translation="MERCGWVSQDPLYIAYHDNEWGVPETDSKKLFEMICLEGQQAGL
                     SWITVLKKRENYRACFHQFDPVKVAAMQEEDVERLVQDAGIIRHRGKIQAIIGNARAY
                     LQMEQNGEPFADFVWSFVNHQPQVTQATTLSEIPTSTSASDALSKALKKRGFKFVGTT
                     ICYSFMQACGLVNDHVVGCCCYPGNKP"
     gene            7373..8071
                     /locus_tag="ACHOSC_RS17125"
                     /old_locus_tag="pgaptmp_003425"
     CDS             7373..8071
                     /locus_tag="ACHOSC_RS17125"
                     /old_locus_tag="pgaptmp_003425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709326.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter domain-containing protein"
                     /protein_id="HGU4549309.1"
                     /translation="MIIKKSGGRWQLSLLASVVISAFFLNTAYAWQQEYIVDTQPGHS
                     TERYTWDSDHQPDYNDILSQRIQSSQRALGLEVNLAEETPVDVTSSMSMGWNFPLYEQ
                     VTTGPVAALHYDGTTTSMYNEFGDSTTTLTDPLWHASVSSLGWRVDSRLGDLRPWAQI
                     SYNQQFGENIWKAQSGLSRMTATNQNGNWLDVTVGADMLLNQNIAAYAALTQAENTTN
                     NSDYLYTMGVSARF"
     gene            8300..9502
                     /locus_tag="ACHOSC_RS17130"
                     /old_locus_tag="pgaptmp_003426"
     CDS             8300..9502
                     /locus_tag="ACHOSC_RS17130"
                     /old_locus_tag="pgaptmp_003426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HGU4549310.1"
                     /translation="MTPSNYQRTRWLTLIGTIITQFALGSVYTWSLFNGALSAKLDAP
                     VSQVAFSFGLLSLGLAISSSVAGKLQERFGVKRVTMASGILLGLGFFLTAHSNNLMML
                     WLSAGVLVGLADGAGYLLTLSNCVKWFPERKGLISAFAIGSYGLGSLGFKFIDTQLLE
                     TVGLEKTFVIWGAIALVMIVFGATLMKDAPKQEVKTSNGVVEKDYTLAESMRKPQYWM
                     LAVMFLTACMSGLYVIGVAKDIAQSLAHLDVVSAANAVTVISIANLSGRLVLGILSDK
                     IARIRVITIGQVISLVGMAALLFAPLNAVTFFAAIACVAFNFGGTITVFPSLVSEFFG
                     LNNLAKNYGVIYLGFGIGSICGSIIASLFGGFYVTFYVIFALLILSLALSTTIRQPEQ
                     KVLREAHI"
BASE COUNT         2245 a         2349 c         2494 g         2466 t
ORIGIN      
        1 ccgccaaatg gcagggatct tagattctgt gcttttaagc agagattaca ggctggttac
       61 gttaccagct gccgggcctt tagcgccgct ttcgatggtg aaggacactt tctgaccttc
      121 gtccagagat ttgtaaccat cgttctggat agcagagaag tgtacgaaca cgtctttaga
      181 gccatcgtca ggagtgatga agccgaagcc tttgtcagcg ttgaaccatt ttacgatacc
      241 agtcatttta ccggacatag tgtattacct ttaataatta agtgtgcctt tcggcgatat
      301 ggcgtgcttt acagattttg aagcgttaaa ggaatgtgca ctacgagggg tatcaacgat
      361 aactcttgaa gggacttgcc ttactacact gctttaatgg tctgtacgtc aaaccgttga
      421 tgtgcattaa gccacgcatt ggcgggtgat gcaacaatta tttttcatat ttatgattaa
      481 tcggttggaa aacggtcctg tcatcaggac cgtaaacagc aataaagtgg ataaaagtct
      541 attccatcaa ctgcttactt aatgccgggt tggcttgaat caaacgcatc aattttagtt
      601 cggcacttga aggcttcacg cgtctggatt cccattcctt taccatggcg actgatacgc
      661 ccaaaacccg ggcgaaatca tcgattttta accctgtccc ttttcgtaac tgctcaattt
      721 cggtacagga cgttgttctg tgcgtcaggg taattttctg cgtttcatct ttaaaaacaa
      781 tctgttccag gctgctcaac agctcatgca ttggatcttt atattccatt gaaaactcct
      841 caaatcacac tgcgggatcg tgaattgcat cgaagctcat taagaatagt cgggaaaagc
      901 agacacagcg gggtgaagtc agggcatgct ctggctatga ttcgattcag ccagtcattt
      961 agcgtgactt atagccacct aacgatctga taaatgccga tatatctctt accactcctg
     1021 gtttgtaaag atttcgaaaa tctaaccttc tgcgaaggcg taaatctctg gctacaaaaa
     1081 aagcaacggc actatcattt tttaacaatg aaacaacaaa ttaaccttgt ttcattttct
     1141 ccatgatttt accaggaaga ttacgtagaa agggtatcct gcaagcctgt cctggactat
     1201 ccttgtcacg tcagacacgc gtgtgtcgtt gtgcgctttt tttgggtgaa aggagtaaga
     1261 aaatggcgac aggaaagtcc tgctctcgct ggtttgcgcc tcttgcggcg ttattaatgg
     1321 tagttagcct gagtgggtgt tttgataaag aaggcgatca gcgtaaagcg tttatcgact
     1381 tcctgcagaa tacagtgatg cgtagcggtg aacgtctacc aaccctgact gccgatcaga
     1441 aaaaacagtt tggtcctttt gtctctgatt acgcgattct gtatggttat tctcagcagg
     1501 tgaatcaggc gatggattcc ggtctgcgcc cggtcgtaga cagcgttaac gcaattcgcg
     1561 taccgcagga ttacgttacg caaagcggtc cgctgcgtga aatgaacggt tcgctgggtg
     1621 tgctggcgca acaactgcaa aatgcgaagt tgcaggccga tgctgcgcac tctgcgctaa
     1681 aacagaccga tgatttgaaa ccggtctttg atcaggcatt caccaaagtg gtaacgacgc
     1741 cagctgatgc attgcaaccg ttgattccgg ctgcacaaac tttcacgcaa caactggtga
     1801 tggttggaga ctacattgct cagcaaggta ctcaggtgag ctttgtcgca aatggcatcc
     1861 agttcccgac ttcacagcag gcaagtgaat ataacaaact gattgcgcca ttaccggcac
     1921 aacatcaggc gtttaatcag gcctggacta cagcagttac tgcaacccaa taaagagtaa
     1981 aagcccgagc gattatcggg ctttacttca cgcagtcgcg gcttagtccg cgacgtgcgg
     2041 attcacacag ttcttctcaa cctttccttg taacgcatca atcaaattat ccacggcaca
     2101 ggcggccatg ccataacgcg tctcatgggt ggcagatcca atatgcggta ctgcgacgac
     2161 gttggccatt gagagcaacg gcgaatctac ggacagtggc tcttgttcga agacatccag
     2221 cccggcggcg tgaatttcgc ctttctgcaa tgcagcaatc agtgcatttt catcaaccac
     2281 cgggccacgt ccggcattaa tgaaaatggc ggaggatttc attttggcga attgttctgc
     2341 gccaaacaga tgatgcgtct catcagttaa cggcaggatc aggcaaacga aatctgactc
     2401 ttgtaacaga gtatccaaat cgcagtagcg ggcgttgaag cgttcttctg cttctttatg
     2461 gtggcggcgc gcgttataga ggatgggcat gttgaagcca aagtgcgcac gttgtgccag
     2521 cgccataccg atccgtccca tcccgacaat gcccagtgtt ttatggtgaa cgtcagtgcc
     2581 gtaccagtcc gggcctatgc tcgcggtcca ttcgcctgct tttacccgtt ctgctacctc
     2641 cacaacccga cgagcggtag acaacaccag cgccatcagc gtatcggcga cggtttctgt
     2701 taatacggtt ggcgtgtgca tcagcagaat ttttcgggcg gtaagcgcat cgacatcaaa
     2761 attgtcatag ccgacggaga tcgttgatgt ggcacgcagt ttcggcattt tttccagcaa
     2821 tgcggcatca acattctcgt ttgaacccag taaaccttca gcttcggcaa aaattgctgc
     2881 attttgttcg acggtttgtg ggctgaggtt tgccacctgg tgaacggtga aatgctcttg
     2941 caggcgttgc agtaaatcat caggtaaggc tttgtagagg ataacggacg gcttcatgct
     3001 tctctccatt cactgataag gcgatcacct tagcctgaaa tatgatgaag gaaaactgaa
     3061 gaatgccagg tgatttctgc atcacctggc atgaaaggga ttacagcggg cttaaggtaa
     3121 tttctacacg gcggttttgc gccttacctt ctgcggtgct gttgctggcg attgggttag
     3181 ccgggccaag gccctgagta cggatgcggc tggcgtccac gccctgggtg atcaacgcgc
     3241 tggcaacgga atccgcacgt tgctgggaga gacgcatgtt caggtcgtga ccacccgtgc
     3301 tgtcggtata accaatcacg ttaaccgccg ttttcggata ctctttcagc accattgcca
     3361 cgccggtcag ggtgttagcg cccgcaggtt tcagggtcgc gctgctgctg tcgaaggtca
     3421 cattgttcgg catattgagg ataatgttat ccccgctgcg ggttacgcta acaccagtgc
     3481 cgcgcatttt gtcacgcagc ttcgcttcct gcacatccat gtaataaccc acgccgccgc
     3541 ccagagctgc gcctgctgct gcgccaatca gcgcgccttt accgcgatct ttcttcgaag
     3601 aagagagcgc accaataccc gcgcccacga gagagcccag acctgcgccg atagcagatt
     3661 tacctgcttc gcgttcgccg gtgtaagggt tagttgtgca gccagatacc gccagagcgc
     3721 cactcactac ggcggcaata agataaacac gtttcttcat tgttaatcct taataacctt
     3781 tttattcttt gccacgggtt ccgtggcggg agattatgcc gcgtgaacat gaagattatt
     3841 cctgggaata ctcggaaatt tgtaagtaat atttaactgc tcaatacatc taacctttca
     3901 ggagttttcg gtttggccaa ctcatcctca cgatattccg ttctgactgc cgcccattgg
     3961 gggcccatgc tggttgaaac cgacggcgaa accgtgttta gctcgcatgg cgcgttagcc
     4021 acaggaatgg aaaactcctt gcagagcgcg gttcgcgacc aggttcacag caatacacgg
     4081 gtacgatttc caatggtgcg caaaggcttt cttgcgtcac cggaaaatcc gcaaggcatt
     4141 cgtgggcagg atgaatttgt tcgcgtgagt tgggatgagg cgctggatct tattcaccaa
     4201 caacataaac gcattcgtga ggcttatggt ccggcatcga tttttgctgg ttcctacggc
     4261 tggcgttcaa acggcgtgct gcataaggcc tcgacattat tacaacgcta tatggcgctg
     4321 gcaggcggtt ataccgggca tctgggggat tattcgaccg gcgcggcaca ggcgatcatg
     4381 ccgtatgtcg tgggtggtag cgaagtttat caacagcaaa ccagttggcc gctggtgctg
     4441 gaacatagcg atgtcgtggt gttgtggagt gctaacccac tcaatacgct gaaaattgcg
     4501 tggaatgcat ctgatgagca ggggctttct tacttttccg cactgcgtga cagcgggaaa
     4561 aagctgatct gtattgatcc aatgcgatcg gaaaccgtcg atttctttgg cgataaaatg
     4621 gagtgggtgg caccgcacat gggcaccgat gttgcgctga tgctggggat cgcccatacg
     4681 ctggtggaaa atggttggca cgacgaagcg tttctgacgc gttgcaccac aggttatgcc
     4741 gtcttcgcct cttatttgct gggcgagagt gacggaatag cgaaaaacgc cgaatgggcg
     4801 gcagagattt gtggggttaa cgcagcgaaa atccgcgagc tggcggctct tttccaccaa
     4861 aacacgacca tgctgatggc tggctgggga atgcagcgcc aacagtttgg tgagcaaaaa
     4921 cactggatga tcgtcacgct ggcggcaatg ttggggcaaa tcggcacacc cggcggcggt
     4981 tttggtcttt cttatcattt tgccaatggt ggtaacccca cgcggcgttc tgcggtgctc
     5041 tcttccatgc agggcagctt gccgggtggc tgcgatgcgg tggataaaat ccctgttgcc
     5101 cgcattgttg aagcactgga aaaccctggt ggcgcatatc aacacaacgg tatgaaccga
     5161 catttcccgg atattcgttt tatctggtgg gcgggcggtg ccaactttac tcatcatcag
     5221 gataccaatc gtctgatccg tgcctggcaa aaaccggagc tggtggtgat ctctgaatgc
     5281 ttctggacgg cggcggcgaa acacgcggat atcgttctgc ctgcgactac ctcttttgag
     5341 cgtaatgatc tcaccatgac cggtgattac agtaatcagc atctggtgcc gatgaagcaa
     5401 gtggtgccgc cacgctatga agcgcgtaat gattttgatg tttttgccga gttgagtgaa
     5461 cgttgggaga agggcggcta tgcgcggttt acggaaggaa aaagtcagct gcaatggctg
     5521 gaaacgtttt ataacgttgc ccggcagcgc ggggcaagcc agcaggttga attgccgcca
     5581 tttgctgagt tctggcaagc caaccagtta attgagatgc cggaaaaccc ggacagcgag
     5641 cggtttattc gcttcgctga tttttgccgc gatccgctgg cgcatccgtt aaaaaccgcc
     5701 agcggcaaga ttgaaatctt ctcacagcgt attgccgatt acggttaccc ggattgccct
     5761 gggcatccaa tgtggctgga gccggacgaa tggcagggca atgccgaacc agaacagttg
     5821 caggtacttt ctgcccatcc ggcgcaccgc ctgcacagcc agctgaatta cagttctctg
     5881 cgcgaattgt acgcggtagc aaaccgtgag cctgtcacca ttcatcctga cgatgctcag
     5941 gcgcgcggca taaccgaagg ggatatggtg cgggtgtgga actcccgtgg gcagatcctt
     6001 gccggagcgg tcattagcga gggaattaaa cctggcgtga tttgcattca cgaaggggca
     6061 tggccggatc tggatttaac cgctgacggt atttgtaaaa acggcgcagt gaacgtgctg
     6121 accaaagatc tccccagctc gcggctgggg aatggctgtg cgggtaatac ggcgctggca
     6181 tggctggaaa aatacaacgg tccggaactg acacttacag cgtttgaacc accggccagc
     6241 tcataatcca ggtgggtagt tgggtttcat cctgccatgc gcaatcgaca atgtgaaaac
     6301 cctgcgcacg gtaaaaatct atcgccggtt gatttttttg ataaacctcc agcatcagat
     6361 ggggatagcg ctgctgcaca tactgcatca gcgccttacc aataccgcgt ctgacggcct
     6421 tcggtgcgac aaacattgct gccagaaatc ggccttccat aatgctgaca aaaccgagaa
     6481 gcttaccgtc ctcttcccag acccagtttt gcgcgttggc aagataggca tcccgcacca
     6541 gcggaatgca gtcacgccag taattcgctt ttataaaggg atgcccccag gttgtacttt
     6601 ccagccacag ttcgaggatc gcggggagtt ctgaacgttg cgcttcccga atcatggttt
     6661 atttcccgga tagcaacagc agccaaccac atgatcattc accagcccac atgcctgcat
     6721 aaaggagtaa cagattgtgg tgccgacaaa cttaaaacca cgttttttca gtgccttaga
     6781 tagggcgtcg gaggcggacg tagatgtggg aatttcgctc aacgttgtgg cttgtgtcac
     6841 ctgtggctga tgatttacaa acgaccagac aaagtcggca aacggttcgc cgttctgttc
     6901 catttgcagg tacgcccgcg cattaccaat aattgcctga attttccctc gatggcggat
     6961 aatcccggcg tcctgtacca gtctttcgac atcctcttcc tgcattgctg cgaccttcac
     7021 cggatcgaac tgatgaaagc aggcgcgata gttttcgcgt tttttgagga cggtgatcca
     7081 cgataaccca gcctgctgcc cttcaaggca gatcatttcg aacagttttt tactgtcagt
     7141 ttcaggcacg ccccattcat tatcatggta ggcaatataa agcgggtcct gactcaccca
     7201 gccgcaacgt tccatacttt ccctcgctat gtacccgatc gtaaaaattt cagccgcttt
     7261 tcttgacgca tcggtgaaaa agtcaatatt taatacaggg ctactatgcc cgatatcctt
     7321 catgcaatga caataatatt gccgaattcg gctcttccct ggagtcgtgt taatgatcat
     7381 aaaaaaaagc ggtggtcgct ggcagctaag cctgctggcg agcgtggtaa tcagtgcctt
     7441 ttttctcaac acagcttacg cctggcaaca agaatatatc gttgatacgc aacccggaca
     7501 ttccacagag cgttacacct gggatagtga tcatcaacct gattacaacg atattttgtc
     7561 gcaacgtatt caaagtagcc aaagggcgct gggactggaa gtcaatctgg cggaagaaac
     7621 tcctgtggat gtgaccagca gtatgagtat gggctggaat tttcctttat atgaacaggt
     7681 tacaaccggc ccggtcgcgg cattacatta cgatggcaca accacctcga tgtataacga
     7741 gtttggcgac agtactacca cgctgaccga tccgttatgg catgccagcg tgagtagctt
     7801 aggctggcgt gttgactccc ggcttggcga tctccgaccc tgggcgcaaa tcagctataa
     7861 ccagcaattt ggcgagaata tctggaaggc gcaatcaggc ctgagccgga tgacggcgac
     7921 aaaccagaac ggcaactggc tggatgtcac cgtaggcgct gatatgttgc tcaatcaaaa
     7981 tattgccgcc tatgccgcgc taactcaggc agaaaatacc actaataata gcgactatct
     8041 gtatacgatg ggggttagcg ccagatttta acgtaacagt cacaatttaa accattgaat
     8101 aacaacagtt gtggcgatag tgggtgctaa cttaccaaat aataaatttg gtgaataatt
     8161 gtcgcgtcat tcattcctga actaaggcat ttcattccgt tctgatggca tttcatgccg
     8221 tttttcccca ggcataaaat gcacttcgtt atggttgtcg gcagagattt ttccttttta
     8281 ttactgcagg aatactgcca tgacaccttc aaattatcag cgtacccgct ggctgacact
     8341 catcggtact atcattaccc agtttgcact ggggtcggtt tatacctgga gcctgtttaa
     8401 tggcgcgctt tctgccaagc tggatgcgcc ggtaagccag gtcgctttct ctttcggctt
     8461 gttaagtctg gggctggcaa tttcgtcttc tgttgcgggc aaattgcagg aacgttttgg
     8521 cgttaaacgc gtcaccatgg cttccggcat tttgctggga ttaggcttct tcctgaccgc
     8581 gcattccaac aacctgatga tgctgtggtt aagcgccggt gtgctggtcg gtctggcgga
     8641 tggcgcgggt tacctgctga cgctctctaa ctgcgtgaag tggttcccgg agcgtaaagg
     8701 tctgatatcc gcgttcgcta tcggttctta tggtctgggt agcctgggtt tcaaatttat
     8761 cgacacgcag ctgctggaaa cggtgggtct ggaaaaaacc tttgtgattt ggggagcgat
     8821 tgcgctggtg atgattgttt tcggcgcaac gttaatgaaa gacgcaccaa aacaggaagt
     8881 gaaaaccagc aatggtgtgg tggagaaaga ttacacgctg gcagagtcga tgcgtaaacc
     8941 gcagtactgg atgttagcgg taatgttcct gaccgcctgc atgagcggcc tgtacgtgat
     9001 tggggtagcg aaagatatcg cccaaagtct ggcacacctt gatgtggttt ccgcagccaa
     9061 tgcagtcact gttatttcca tcgccaacct ttcaggtcgt ctggtgctgg gtattctgtc
     9121 tgacaaaatc gcccgtatcc gtgttatcac cattggtcag gtgatatcgc tggtgggtat
     9181 ggcggccctg ctgtttgcac cattgaatgc agtgacgttc tttgcagcga ttgcctgcgt
     9241 ggcatttaac tttggcggca ctattaccgt ctttccgtca ctggtcagtg agttctttgg
     9301 cctcaataac ctggcgaaaa actacggtgt gatttacctc ggtttcggta tcggtagcat
     9361 ttgtggttcg attatcgcct cactgtttgg cggcttctat gtgaccttct acgtgatttt
     9421 cgccctgctg attctgtcat tggcgctttc tacgacaatt cgtcagccag agcagaaagt
     9481 attgcgtgag gcgcatattt agaaatatgt tgctactgaa ataaatgcga ggagggagac
     9541 ttcaaagtct ccct
//
LOCUS       DAZKHF020000177         9404 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2201S31241
            SAMN43267800-rid25106413.denovo.178, whole genome shotgun sequence.
ACCESSION   DAZKHF020000177 DAZKHF020000000
VERSION     DAZKHF020000177.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267800
            Sequence Read Archive: SRR30307263
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 9404)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9404)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 9404)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350581.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350581.2
            Genome Coverage       :: 151x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:21:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,652
            CDSs (total)                      :: 5,557
            Genes (coding)                    :: 5,320
            CDSs (with protein)               :: 5,320
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 237
            CDSs (without protein)            :: 237
            Pseudo Genes (ambiguous residues) :: 0 of 237
            Pseudo Genes (frameshifted)       :: 102 of 237
            Pseudo Genes (incomplete)         :: 130 of 237
            Pseudo Genes (internal stop)      :: 52 of 237
            Pseudo Genes (multiple problems)  :: 41 of 237
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9404
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267800-rid25106413.denovo.178"
                     /serotype="O 26:H11"
                     /isolate="2201S31241"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-01"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            140..1204
                     /gene="ulaG"
                     /locus_tag="ACHOSC_RS17135"
                     /old_locus_tag="pgaptmp_003427"
     CDS             140..1204
                     /gene="ulaG"
                     /locus_tag="ACHOSC_RS17135"
                     /old_locus_tag="pgaptmp_003427"
                     /EC_number="3.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003856017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030145 - manganese ion binding [Evidence
                     IEA];
                     GO_function: GO:0035460 - L-ascorbate 6-phosphate
                     lactonase activity [Evidence IEA];
                     GO_process: GO:0019854 - L-ascorbic acid catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-ascorbate 6-phosphate lactonase"
                     /protein_id="HGU4549311.1"
                     /translation="MSKVKSITRESWILSTFPEWGSWLNEEIEQEQVAPGTFAMWWLG
                     CTGIWLKSEGGTNVCVDFWCGTGKQSHGNPLMKQGHQMQRMAGVKKLQPNLRTTPFVL
                     DPFAIRQIDAVLATHDHNDHIDVNVAAAVMQNCADDVPFIGPKTCVDLWIGWGVPKER
                     CIVVKPGDVVKVKDIEIHALDAFDRTALITLPADQKAAGVLPDGMDDRAVNYLFKTPG
                     GSLYHSGDSHYSNYYAKHGNEHQIDVALGSYGENPRGITDKMTSADMLRMGEALNAKV
                     VIPFHHDIWSNFQADPQEIRVLWEMKKDRLKYGFKPFIWQVGGKFTWPLDKDNFEYHY
                     PRGFDDCFTIEPDLPFKSFL"
     gene            1312..2067
                     /gene="ulaR"
                     /locus_tag="ACHOSC_RS17140"
                     /old_locus_tag="pgaptmp_003428"
     CDS             1312..2067
                     /gene="ulaR"
                     /locus_tag="ACHOSC_RS17140"
                     /old_locus_tag="pgaptmp_003428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator UlaR"
                     /protein_id="HGU4549312.1"
                     /translation="MTEAQRHQILLEMLAQLGFVTVEKVVERLGISPATARRDINKLD
                     ESGKLKKVRNGAEAITQQRPRWTPMNLHQAQNHDEKVRIAKAASQLVNPGESVVINCG
                     STAFLLGREMCGKPVQIITNYLPLANYLIDQEHDSVIIMGGQYNKSQSITLSPQGSEN
                     SLYAGHWMFTSGKGLTAEGLYKTDMLTAMAEQKMLSVVGKLVVLVDSSKIGERAGMLF
                     SRADQIDMLITGKNANPEILQQLEAQGVSILRV"
     gene            complement(2064..2813)
                     /gene="yjfP"
                     /locus_tag="ACHOSC_RS17145"
                     /old_locus_tag="pgaptmp_003429"
     CDS             complement(2064..2813)
                     /gene="yjfP"
                     /locus_tag="ACHOSC_RS17145"
                     /old_locus_tag="pgaptmp_003429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="esterase"
                     /protein_id="HGU4549313.1"
                     /translation="MIEIESRELADIPVLHAYPVGQKDTPLPCVIFYHGFTSSSLVYS
                     YFAVALAQAGLRVIMPDAPDHGSRFSSDEARRLNQFWQILLQSMQEFTTLRAAIAEEN
                     WLLDDRLAVGGASMGAMTALGITTRHPTVKCTASMMGSGYFTSLARSLFPPLIPETAA
                     QQNEFNNIIAPLAEWEATNHLEQLGDRPLLLWHGLDDDVVPADESLRLQQALSETGRD
                     KLLTCSWQPGVRHRITPEALDAAVTFFRQHL"
     gene            2995..3324
                     /gene="bsmA"
                     /locus_tag="ACHOSC_RS17150"
                     /old_locus_tag="pgaptmp_003430"
     CDS             2995..3324
                     /gene="bsmA"
                     /locus_tag="ACHOSC_RS17150"
                     /old_locus_tag="pgaptmp_003430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313192.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biofilm peroxide resistance protein BsmA"
                     /protein_id="HGU4549314.1"
                     /translation="MVSRKRNSVIYRFASLLLVLMLSACSALQGTPQPAPPVTNHPQE
                     IRRDQTQGLQRIGSVSTMVRGSPDDALAEIRAKAVAAKADYYVVVMVDETIVTGQWYS
                     QAILYRK"
     gene            3473..3748
                     /gene="yjfN"
                     /locus_tag="ACHOSC_RS17155"
                     /old_locus_tag="pgaptmp_003431"
     CDS             3473..3748
                     /gene="yjfN"
                     /locus_tag="ACHOSC_RS17155"
                     /old_locus_tag="pgaptmp_003431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418609.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1471 family protease activator YjfN"
                     /protein_id="HGU4549315.1"
                     /translation="MKQLLASPSLQLVTYPASATAQSAEFASADCVTGLNEIGQISVS
                     NISGDPQDVERIVALKADEQGASWYRIITMYEDQQPDNWRVQAILYA"
     gene            complement(3865..5490)
                     /gene="aidB"
                     /locus_tag="ACHOSC_RS17160"
                     /old_locus_tag="pgaptmp_003432"
     CDS             complement(3865..5490)
                     /gene="aidB"
                     /locus_tag="ACHOSC_RS17160"
                     /old_locus_tag="pgaptmp_003432"
                     /EC_number="1.3.8.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418608.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003995 - acyl-CoA dehydrogenase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isovaleryl-CoA dehydrogenase"
                     /protein_id="HGU4549316.1"
                     /translation="MHWQTHTVFNQPIPLNNSNLYLSDGALCEAVTREGAGWDSDFLA
                     SIGQQLGTAESLELGRLANVNPPELLRYDAQGRRLDDVRFHPAWHLLMQALCTNRVHN
                     LAWEEDARSGAFVARAARFMLHAQVEAGSLCPITMTFAATPLLLQMLPTPFQDWTTPL
                     LSDRYDSHLLPGGQKRGLLIGMGMTEKQGGSDVMSNTTRAERLEDGSYRLVGHKWFFS
                     VPQSDAHLVLAQTTGGLSCFFVPRFLPDGQRNAIRLERLKDKLGNRSNASCEVEFQDA
                     IGWLLGQEGEGIRLILKMGGMTRFDCALGSHAMMRRAFSLAIYHAHQRHVFGNPLIQQ
                     PLMRHVLSRMALQLEGQTALLFRLARAWDRRADAKEALWARLFTPAAKFVICKRGMPF
                     VAEAMEVLGGIGYCEESELPRLYREMPVNSIWEGSGNIMCLDVLRVLNKQAGVYDLLS
                     EAFVEVKGQDRYFDRAVRRLQQQLRKPAEELGREITHQLFLLGCGAQMLKYASPPMAQ
                     AWCQVMLDTRGGVRLSEQIQNDLLLRATGGVCV"
     gene            complement(5574..6737)
                     /gene="yjfC"
                     /locus_tag="ACHOSC_RS17165"
                     /old_locus_tag="pgaptmp_003433"
     CDS             complement(5574..6737)
                     /gene="yjfC"
                     /locus_tag="ACHOSC_RS17165"
                     /old_locus_tag="pgaptmp_003433"
                     /EC_number="6.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathionylspermidine synthase family protein"
                     /protein_id="HGU4549317.1"
                     /translation="MLRHNVPVRRDLDQIAANNGFDFHIIDNEIYWDESRAYRFTLRQ
                     IEEQIEKPTAELHQMCLEVVDRAVKDEEILTQLAIPPLYWDVIAESWRARDPSLYGRM
                     DFAWCGNAPVKLLEYNADTPTSLYESAYFQWLWLEDARRSGIIPRDADQYNAIQERLI
                     SRFSELYSREPFYFCCCQDTDEDRTTVLYLQDCAQQAGQESRFIYIEDLGLGVGGVLT
                     DLDDNVIQRAFKLYPLEWMMRDDNGPLLRKRREQWVEPLWKSILSNKGLMPLLWRFFP
                     GHPNLLASWFEGEKPQIAAGESYVRKPIYSREGGNVTIFDGQNNVVDHADGDYADEPM
                     IYQAFQPLPRFGDSYTLIGSWIVDDEACGMGIREDNTLITKDTSRFVPHYIAG"
     gene            complement(6740..7378)
                     /gene="yjfM"
                     /locus_tag="ACHOSC_RS17170"
                     /old_locus_tag="pgaptmp_003434"
     CDS             complement(6740..7378)
                     /gene="yjfM"
                     /locus_tag="ACHOSC_RS17170"
                     /old_locus_tag="pgaptmp_003434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1190 domain-containing protein"
                     /protein_id="HGU4549318.1"
                     /translation="MARKRKSRNNSKIGHGAISRIGRPNNPFEPRRNRYAQKYLTLAL
                     MGGAAFFVLKGCGDSGDVDNDGDGTFYSTVQDCIDDGNNADICARGWNNAKAAFYADV
                     PKNMTQQNCQSKYENCYYDNVEQSWIPVVSGFLLSRVIRKDRDEPFVYNSGGSSFASR
                     PVWRNTSGDYSWRSGSGKKESYSSGGFTTKKASTVSRGGYGRSSSARGHWGG"
     gene            complement(7388..7786)
                     /gene="yjfL"
                     /locus_tag="ACHOSC_RS17175"
                     /old_locus_tag="pgaptmp_003435"
     CDS             complement(7388..7786)
                     /gene="yjfL"
                     /locus_tag="ACHOSC_RS17175"
                     /old_locus_tag="pgaptmp_003435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF350 domain-containing protein"
                     /protein_id="HGU4549319.1"
                     /translation="MHILDSLLAFSAYFFIGVAMVIIFLFIYSKITPHNEWQLIKNNN
                     TAASLAFSGTLLGYVIPLSSAAINAVSIPDYFAWGGIALVIQLLVFAGVRLYMPALSE
                     KIINHNTAAGMFMGTAALAGGIFNAACMTW"
     gene            complement(7804..8463)
                     /gene="yjfK"
                     /locus_tag="ACHOSC_RS17180"
                     /old_locus_tag="pgaptmp_003436"
     CDS             complement(7804..8463)
                     /gene="yjfK"
                     /locus_tag="ACHOSC_RS17180"
                     /old_locus_tag="pgaptmp_003436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjfK family protein"
                     /protein_id="HGU4549320.1"
                     /translation="MSGFFQRLFGKDNKPAIARGPLGLHLNSGFTLDTLAFRLLEDEL
                     LIALPGEEFTVAAVSHIDLGGGSQIFRYYTSGDEFLQINTTGGEDIDDIDDIKLFVYE
                     ESYGISKESHWREAINAKVMGAMILNWQEKRWQRFFNSEEPGNIEPVYMLEKVENQNH
                     AKWEVHNFTMGYQRQVTEDTYEYLLLNGEESFNDLGEPEWLFSRALGVDIPLTSLHII
                     G"
     gene            complement(8514..9212)
                     /gene="yjfJ"
                     /locus_tag="ACHOSC_RS17185"
                     /old_locus_tag="pgaptmp_003437"
     CDS             complement(8514..9212)
                     /gene="yjfJ"
                     /locus_tag="ACHOSC_RS17185"
                     /old_locus_tag="pgaptmp_003437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PspA/IM30 family protein"
                     /protein_id="HGU4549321.1"
                     /translation="MGILKSLFTLGKSFISQAEESIEETQGVRMLEQHIRDAKAELDK
                     AGKSRVDLLARVKLSHDKLKDLRERKASLEARALEALSKNVNPSLINEVAEEIARLEN
                     LITAEEQVLSNLEVSRDGVEKAVTATAQRIAQFEQQMEVVKATEAMQRAQQAVTTSTV
                     GASSSVSTAAESLKRLQTRQAERQARLDAAAQLEKVADGRDLDEKLAEAGIGGSNKSS
                     AQDVLARLQRQQGE"
     gene            complement(9231..>9404)
                     /locus_tag="ACHOSC_RS17190"
                     /old_locus_tag="pgaptmp_003438"
     CDS             complement(9231..>9404)
                     /locus_tag="ACHOSC_RS17190"
                     /old_locus_tag="pgaptmp_003438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2170 family protein"
                     /protein_id="HGU4549322.1"
                     /translation="KMMPLSSVGISSVQQEEYYIVFGALSLKSSLEDILLEITSLVDN
                     ALDLAEITEEYSH"
BASE COUNT         2438 a         2490 c         2250 g         2226 t
ORIGIN      
        1 ttccacactc aaaatcaaca acaacttccc tggcaaaaag tcaaaaaatg gtcattgcct
       61 tcctgaaaaa atagtgtcag gattaatcaa aaccaatcac atattgattc gaattgaact
      121 atcatgaagg taaatggcga tgagtaaagt gaaaagtatc acccgagaat cctggatcct
      181 gagcactttc ccggagtggg gtagctggct gaatgaagaa attgaacaag agcaggtcgc
      241 ccctggcaca tttgcgatgt ggtggctagg ctgcaccggg atctggttga aatcagaagg
      301 tggcaccaac gtttgcgttg atttctggtg cggcactggc aaacaaagtc acggtaaccc
      361 gttaatgaaa cagggtcacc agatgcagcg catggctggc gtgaaaaaac tgcagccaaa
      421 cctacgcacc accccgtttg ttcttgatcc gtttgcgatt cgccagatcg acgcggtact
      481 ggcgactcac gatcacaacg atcatatcga cgttaacgtc gctgctgccg tgatgcagaa
      541 ctgtgctgat gacgtaccat tcattggacc gaaaacctgc gttgatttgt ggattggctg
      601 gggcgtaccg aaagagcgtt gcatcgtggt caaaccgggc gatgttgtaa aagtaaaaga
      661 cattgaaatt catgcgcttg acgctttcga ccgtactgca ctgatcaccc tgcctgccga
      721 tcaaaaagcg gctggcgtac tgccggacgg catggacgat cgcgcggtga actatctgtt
      781 caaaacgcct ggcggctccc tgtatcacag cggcgactcc cactactcta actattatgc
      841 gaagcacggt aacgaacatc agatcgatgt ggcgttagga tcgtatggtg agaacccgcg
      901 cggtattacc gacaaaatga ccagcgccga tatgctgcgt atgggtgaag ccctgaatgc
      961 gaaagtggtt atcccgttcc accacgacat ctggtcaaac ttccaggccg atccgcaaga
     1021 gatccgcgtg ctgtgggaga tgaaaaaaga tcgtctgaag tatggcttca agccgtttat
     1081 ctggcaggtt ggcggcaaat ttacctggcc gctggataaa gacaacttcg agtaccacta
     1141 tccgcgcggt ttcgatgatt gcttcactat tgaaccggat ctgccgttca agtcattcct
     1201 gtaatctgtc gtttataccg gatagcgtgg agaagttcgc gctatccggc gtagtatttc
     1261 ctggctattt caaatatcat ctaaaaaaat caaattttat cggaatagct catgactgaa
     1321 gcacaaagac atcaaatcct cctggaaatg ctcgcacaat tgggctttgt gaccgttgag
     1381 aaagtcgttg agcgtctggg aatttcgcct gccactgcgc gacgcgatat caataaactt
     1441 gacgaaagcg gcaaactgaa aaaagtgcgc aatggcgcag aagctattac ccagcagcgc
     1501 ccgcgctgga cgccgatgaa tctgcatcag gcgcagaatc acgatgaaaa agtacgtatc
     1561 gctaaagcag cctcgcagct ggttaatccg ggcgaaagcg tagtcatcaa ctgcggctcc
     1621 accgcgtttc tgcttgggcg ggaaatgtgt ggcaagccag tgcaaatcat cactaattat
     1681 ctaccgctgg caaattacct gatcgatcaa gaacatgaca gcgtgatcat tatgggtgga
     1741 cagtacaaca aaagtcagtc catcacttta agcccgcagg gcagcgaaaa cagtctctat
     1801 gccgggcact ggatgtttac cagcggaaaa gggctgaccg cagaagggtt gtataaaacc
     1861 gatatgctga cagcaatggc agagcagaag atgctgagcg tggtagggaa actggtggta
     1921 ctggttgata gcagtaagat tggcgaacgc gcgggaatgc tttttagccg tgccgatcaa
     1981 atcgatatgc tgatcaccgg caaaaatgct aacccggaaa tcctgcaaca actggaagcg
     2041 caaggggtca gcattctgcg tgtttaaaga tgctggcgga aaaatgtcac ggcagcatct
     2101 aacgcctcag gcgtaatgcg gtgacgcacg cctggctgcc atgaacaggt tagcagttta
     2161 tcccgtcccg tctcgcttaa ggcctgctgc aaacgtagtg attcgtcggc aggcacaacg
     2221 tcatcgtcga ggccatgcca cagcagtaga ggtctgtccc caagttgttc caggtggttt
     2281 gtcgcttccc actctgccag cggcgcgata atgttattga attcattctg ctgtgctgcc
     2341 gtttcaggta tcagcggtgg aaacagtgaa cgggcgagcg atgtaaaata gcccgatccc
     2401 atcatgctgg cggtacattt caccgtgggg tggcgagtgg taattcccag tgccgtcatc
     2461 gcgcccatcg acgcgccacc gactgccaga cggtcatcaa gcagccagtt ttcttcggct
     2521 attgccgcac gtaaagtagt gaattcctgc atactttgta gcaagatttg ccagaattga
     2581 tttaaccgcc gcgcttcgtc gctactaaaa cggctaccgt gatcgggcgc atccggcatg
     2641 atcacccgca aaccagcctg cgccagcgca acggcaaaat agctatacac caggctggat
     2701 gaagtaaagc cgtgataaaa aattacgcac ggtaacgggg tatctttttg cccgacagga
     2761 taagcatgaa gaacgggaat atctgccagc tcgcgtgatt ctatttcaat catctgtctc
     2821 tcctttttgt ctggtatgac atgaagaaat ccttagcaca agtttctgct ggcggtatta
     2881 gttcataatg ttgagatgtg ggttacgctt tcgtcacgtt ttcattcgaa aatcgcgtac
     2941 caggtaacaa ttcgggaaca ttcccccaaa gttaaattaa taaggcacta cactatggtt
     3001 agcaggaaac gtaatagcgt tatttaccgg tttgccagtt tattattggt gttgatgtta
     3061 agtgcctgta gcgctctgca aggtacgcca cagccagcac cgccagtaac gaaccatccg
     3121 caagagattc gccgcgacca gacgcaaggg ttacagcgaa taggtagcgt aagcacgatg
     3181 gttcggggtt ctccggatga cgcattagca gaaatcagag caaaagctgt cgctgcaaaa
     3241 gctgattatt acgttgtcgt catggtggac gaaaccatcg tgacaggaca gtggtattca
     3301 caagccattt tgtaccgtaa ataaatcaga caacttacat agtcagcttt acattgcttt
     3361 gcgtccctat gcgtttacct gtgcacaata aattaccggc tggaagcctg ggcgtttatt
     3421 accgcgatgg aatgccctgc aacatgtggg gaaacgaaaa atggagctga cgatgaaaca
     3481 attacttgcc tcaccctcgc tgcaattagt gacttatcct gcgagcgcca cggcacagtc
     3541 tgcagaattc gccagtgctg actgcgtgac gggcctgaat gaaataggtc agatctcagt
     3601 tagcaatatt tcaggggatc cgcaggacgt ggaacgtatc gtggcgttaa aggccgatga
     3661 acaaggtgcg tcatggtacc gcattattac catgtacgaa gaccagcagc ctgataactg
     3721 gcgcgtacag gcgatactgt atgcatgagt gttatataca gcaaatgaac aatgccggat
     3781 gcggcgcgag cgccttgtcc ggcctacaat gttcgcatat ttcattgagt taatcgaaac
     3841 cagcgtcgca tcagtcgtat acgcttacac acacactccc cccgtcgccc gcagcaataa
     3901 atcattctgg atctgctctg acaaccgtac gccgccgcgc gtatctaaca tcacctgaca
     3961 ccacgcctgc gccattggcg gagaggcata tttcaacatt tgcgcaccgc agcccagcag
     4021 gaatagctga tgagtaatct ctcgccccag ttcttcagct ggcttgcgca gctgctgctg
     4081 taaacgacga accgcgcgat caaaatagcg atcctgccct ttcacttcca caaacgcttc
     4141 cgacaataag tcgtatacgc ccgcttgctt attgagaacg cgcagcacat ccaggcacat
     4201 aatattgccg gaaccttccc aaatactgtt taccggcatc tcccggtaaa gccgcggcag
     4261 ctcgctctcc tcgcaataac caatgccgcc cagcacctcc atcgcttcgg ccacaaacgg
     4321 catacctctt ttgcagatca caaattttgc cgcaggcgta aataaacgcg cccacagggc
     4381 ttctttggca tcggcacgcc ggtcccacgc tcgcgcaaga cgaaacagca acgccgtttg
     4441 cccttcaagc tgaagtgcca tgcgacttaa gacatgacgc ataaggggct gttggatcaa
     4501 tggattacca aaaacatggc gttgatgtgc atgataaatc gccagcgaaa atgcacggcg
     4561 catcatggca tggctaccca gggcgcaatc aaaacgcgtc atcccaccca ttttcaggat
     4621 cagacgaatt ccttcccctt cctgccccaa caaccagcca atggcatcct gaaactccac
     4681 ttcgcagctg gcgttagagc gattacccag cttatctttc agccgctcga ggcgaatcgc
     4741 gttgcgttgc ccgtcaggca aaaagcgcgg cacaaaaaag caggacaaac cgcccgtagt
     4801 ctgcgccagc accagatgcg catcgctttg cggcaccgag aaaaaccatt tatgccccac
     4861 cagccgataa gagccatctt ccaggcgctc tgcgcgggtg gtgttgctca tgacatcgga
     4921 accgccctgc ttttccgtca ttcccatgcc aatcaacaaa ccgcgttttt gcccacctgg
     4981 caataagtga gaatcgtagc gatcgctcag cagcggcgtg gtccagtcct gaaacggcgt
     5041 gggtaacatc tgtaacaaca atggcgtggc ggcaaaggtc atcgttattg gacataacga
     5101 cccagcctca acctgcgcat gtaacataaa acgcgccgcg cgcgccacaa atgcgccgga
     5161 gcgagcgtct tcttcccagg caagattgtg cacccgattg gtacataacg cctgcatcag
     5221 caggtgccag gcggggtgaa aacgcacatc gtccagacgg cgtccttgcg catcgtagcg
     5281 caataattca ggcggattca cattcgccag ccgccccagt tcaagagatt cagccgttcc
     5341 taactgctga ccaatactgg ctagaaaatc gctatcccag ccagcacctt cacgcgttac
     5401 cgcttcacag agcgcgccat cagacaggta taagttgctg ttatttaatg gtataggttg
     5461 attaaaaacg gtgtgagttt gccagtgcac tgtgtctccc tccatcaatg gcagtcaccc
     5521 ttagtatggt cactgccatg gattcctgac agaaagagat tgctaaaaca ttcttatcca
     5581 gcaatgtaat gcggaacgaa acgtgaggtg tctttggtga tcagcgtgtt atcttcacgg
     5641 atccccattc cgcacgcttc atcatcgaca atccagctac cgatgagtgt gtagctatcg
     5701 ccaaatcgcg gcagaggttg aaacgcctgg tagatcatcg gttcatcggc gtaatcacca
     5761 tcagcgtggt caacgacgtt attctgaccg tcgaaaatgg tgacgtttcc gccttcgcgc
     5821 gagtagatcg gtttacgcac atagctttcg ccagcggcaa tctgcggttt ttcgccctca
     5881 aaccaggatg caagaaggtt aggatgacca gggaagaagc gccaaagcag cggcattagc
     5941 cctttattac tcaagatact tttccataac ggctccaccc attgctcgcg acgcttgcgc
     6001 agcagcggac cgttatcgtc acgcatcatc cactccagcg gatacagctt aaatgcacgc
     6061 tggatgacat tatcatcaag atcggtcagt acaccgccga cgcccaaacc gagatcttca
     6121 atgtagataa accgcgactc ctgccctgcc tgctgggcgc agtcctgcaa gtacagcacg
     6181 gtagtcctgt cttcatcggt gtcctgacag cagcagaaat aaaacggctc ccggctataa
     6241 agctcgctaa agcgtgaaat caggcgttcc tgaatggcat tgtactgatc ggcatcacgc
     6301 ggaataatgc cgctgcgccg ggcatcctcc agccacaacc actggaaata agccgactca
     6361 tacaacgagg tcggcgtatc ggcgttgtac tccagcagct tcaccggcgc attgccacac
     6421 caggcaaaat ccatccggcc atacagcgaa ggatcgcgtg cgcgccagct ttcggcgatc
     6481 acatcccagt acaacggcgg gatcgccagt tgcgtcagga tctcttcgtc tttaaccgcg
     6541 cgatcaacca cctcaaggca catctgatgc agttccgcag tcggtttttc gatctgctct
     6601 tcaatctggc gcagagtaaa acggtaagcc cgactctcgt cccaatagat ttcattgtcg
     6661 atgatatgaa agtcgaaacc gttgttagcg gcgatctggt ccagatcccg tcgcacagga
     6721 acgttgtgtc tcagcatgat tagcctcccc aatgcccacg ggcgctggaa gaacgaccat
     6781 agccgccgcg cgaaacggtt gacgcttttt tggtggtaaa gccgcctgaa gagtaagact
     6841 cttttttgcc agaaccggag cgccaggagt aatcaccgga agtgttgcgc cagacggggc
     6901 gggaagcaaa tgaggatccg ccgctgttat agacgaacgg ctcatcgcga tctttacgaa
     6961 taacccggct taacaaaaat ccagaaacga ccgggatcca actctgttca acattgtcgt
     7021 agtagcaatt ttcgtactta gactgacaat tctgctgagt catattcttc ggaacatcgg
     7081 cataaaatgc cgctttggcg ttgttccagc cacgcgcgca aatgtcggca ttattaccgt
     7141 catcaataca atcctgcact gtcgaataaa acgttccgtc gccgtcgtta tcgacatcgc
     7201 cactatcgcc acaacctttc aatacaaaaa aagcggcacc gcccattaga gccaacgtta
     7261 agtatttttg tgcgtagcga ttgcgacgcg gttcaaaagg attattcggt ctaccaatgc
     7321 gactgatcgc tccgtgacca attttactgt tatttcttga tttgcgtttt ctggccatga
     7381 tcgtccatta ccatgtcata caagctgcgt taaaaatacc gccagccagc gcggcggttc
     7441 ccataaacat tcctgctgcg gtattgtgat taataatttt ttcgcttaat gcgggcatat
     7501 aaagtctgac accagcaaaa acgagtaact gaatcaccag tgcaatcccg ccccaggcga
     7561 aatagtctgg aatactcacc gcattgattg ccgcactgga taatgggata acgtaaccta
     7621 acaatgtacc actgaacgcc agtgaagccg cggtattatt gtttttgatt aactgccatt
     7681 cgttgtgcgg tgtaatttta gaatagataa aaagaaaaat aatcaccata gccacgccaa
     7741 taaaaaaata ggcgctaaag gcaagaagtg aatccagtat atgcattgta aacgtccttg
     7801 taattaacca ataatatgaa gtgatgtcag cgggatatcg acacccaacg cgcgcgaaaa
     7861 taaccactct ggctcgccga gatcgttaaa agattcctca ccatttaaca gcaaatattc
     7921 gtaagtatct tcggttactt gccgctggta gcccatggta aaattatgga cctcccattt
     7981 ggcatggttt tgattttcta ctttttccag catgtagacg ggttcgatat ttcccggttc
     8041 ttcgctgtta aaaaatcgct gccagcgttt ttcctgccag tttaagatca ttgcccccat
     8101 cactttggcg ttgatcgcct cgcgccagtg actctcttta ctgataccgt agctctcttc
     8161 atagacaaag agcttgatat catcaatgtc atcaatatct tcgccgccgg tggtattgat
     8221 ttgcagaaat tcatcgcctg aagtgtagta gcggaaaatc tgactaccgc cgcccagatc
     8281 gatgtggctg acagcggcta ccgtaaattc ctcacccggc agcgcaatca gcaattcatc
     8341 ttccagcaaa cgaaacgcta acgtgtcgag cgtaaagcca ctattgagat gaagtcccag
     8401 cggaccgcga gcgatagcag gcttattatc cttgccaaac agacgctgga aaaaaccaga
     8461 catattttat ccttaaaata gttgccagcc ttttagcgag gctggcaaaa aaattactcg
     8521 ccctgttggc gttgcagtct tgctaataca tcctgggcgc tacttttatt gctaccgcca
     8581 attccggctt ccgccagctt ttcatcaaga tcgcgaccgt ctgcgacttt ttccaactgt
     8641 gcagcagcat ccaggcgagc ctgacgttcg gcctgacgcg tttgcaggcg ttttaaggat
     8701 tctgccgctg tcgaaacgct ggaagatgcg ccaacggtag aggttgttac agcctgttgt
     8761 gcacgctgca tggcttcagt ggctttaacg acttccattt gctgctcaaa ctgagcgata
     8821 cgctgcgctg tcgctgtaac tgctttttcc acgccatcac gggagacttc cagattcgac
     8881 aacacttgct cttcagcggt aatgagattc tcaaggcgcg cgatttcttc agcaacttcg
     8941 ttaatcaacg acggattaac gttcttgctt aacgcttcca gcgcacgggc ttccagactg
     9001 gctttgcgct cacgtaaatc tttcagctta tcgtgactta atttcacccg cgccagcaga
     9061 tcaacgcgag attttccggc tttatcgagt tcagctttag cgtcacgaat atgctgttcc
     9121 agcatgcgca cgccctgggt ttcttcaatg gattcttccg cctgggagat aaacgatttc
     9181 cccagcgtaa ataaactttt taaaattccc ataaataccc gtccttgtaa ttagtgtgaa
     9241 tattcttctg taatttcagc cagatccaat gcgttatcta ccagcgaggt tatctcaagc
     9301 aggatatctt caagagaaga tttaagcgat aacgccccga aaacaatgta atactcttcc
     9361 tgttgcacgc tggagattcc taccgatgac aacggcatca tctt
//
LOCUS       DAZKHF020000178         9229 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2201S31241
            SAMN43267800-rid25106413.denovo.179, whole genome shotgun sequence.
ACCESSION   DAZKHF020000178 DAZKHF020000000
VERSION     DAZKHF020000178.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267800
            Sequence Read Archive: SRR30307263
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 9229)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9229)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 9229)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350581.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350581.2
            Genome Coverage       :: 151x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:21:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,652
            CDSs (total)                      :: 5,557
            Genes (coding)                    :: 5,320
            CDSs (with protein)               :: 5,320
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 237
            CDSs (without protein)            :: 237
            Pseudo Genes (ambiguous residues) :: 0 of 237
            Pseudo Genes (frameshifted)       :: 102 of 237
            Pseudo Genes (incomplete)         :: 130 of 237
            Pseudo Genes (internal stop)      :: 52 of 237
            Pseudo Genes (multiple problems)  :: 41 of 237
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9229
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267800-rid25106413.denovo.179"
                     /serotype="O 26:H11"
                     /isolate="2201S31241"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-01"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            205..1836
                     /gene="oppA"
                     /locus_tag="ACHOSC_RS17195"
                     /old_locus_tag="pgaptmp_003439"
     CDS             205..1836
                     /gene="oppA"
                     /locus_tag="ACHOSC_RS17195"
                     /old_locus_tag="pgaptmp_003439"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707150.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptide ABC transporter substrate-binding
                     protein OppA"
                     /protein_id="HGU4549323.1"
                     /translation="MTNITKRSLVAAGVLAALMAGNVALAADVPAGVTLAEKQTLVRN
                     NGSEVQSLDPHKIEGVPESNISRDLFEGLLVSDLDGHPAPGVAESWDNKDAKVWTFHL
                     RKDAKWSDGTPVTAQDFVYSWQRSVDPNTASPYASYLQYGHIAGIDEILEGKKPITDL
                     GVKAIDDHTLEVTLSEPVPYFYKLLVHPSTSPVPKAAIEKFGEKWTQPGNIVTNGAYT
                     LKDWVVNERIVLERSPTYWNNAKTVINQVTYLPIASEVTDVNRYRSGEIDMTYNNMPI
                     ELFQKLKKEIPDEVHVDPYLCTYYYEINNQKPPFNDVRVRTALKLGMDRDIIVNKVKA
                     QGDMPAYGYTPPYTDGAKLTQPEWFGWSQEKRNEEAKKLLAEAGYTADKPLTINLLYN
                     TSDLHKKLAIAASSLWKKNIGVNVKLVNQEWKTFLDTRHQGTFDVARAGWCADYNEPT
                     SFLNTMLSNSSMNTAHYKSPAFDSIMAETLKVTDEAQRTALYTKAEQQLDKDSAIVPV
                     YYYVNARLVKPWVGGYTGKDPLDNTYTRNMYIVKH"
     gene            1922..2842
                     /gene="oppB"
                     /locus_tag="ACHOSC_RS17200"
                     /old_locus_tag="pgaptmp_003440"
     CDS             1922..2842
                     /gene="oppB"
                     /locus_tag="ACHOSC_RS17200"
                     /old_locus_tag="pgaptmp_003440"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptide ABC transporter permease OppB"
                     /protein_id="HGU4549324.1"
                     /translation="MLKFILRRCLEAIPTLFILITISFFMMRLAPGSPFTGERTLPPE
                     VMANIEEKYHLNDPIMTQYFSYLKQLAHGDFGPSFKYKDYSVNDLVASSFPVSAKLGA
                     AAFFLAVILGVSAGVIAALKQNTKWDYTVMGLAMTGVVIPSFVVAPLLVMIFAIILHW
                     LPGGGWNGGALKFMILPMVALSLAYIASIARITRGSMIEVLHSNFIRTARAKGLPMRR
                     IILRHALKPALLPVLSYMGPAFVGIITGSMVIETIYGLPGIGQLFVNGALNRDYSLVL
                     SLTILVGALTILFNAIVDVLYAVIDPKIRY"
     gene            2857..3765
                     /gene="oppC"
                     /locus_tag="ACHOSC_RS17205"
                     /old_locus_tag="pgaptmp_003441"
     CDS             2857..3765
                     /gene="oppC"
                     /locus_tag="ACHOSC_RS17205"
                     /old_locus_tag="pgaptmp_003441"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000979653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptide ABC transporter permease OppC"
                     /protein_id="HGU4549325.1"
                     /translation="MMLSKKNSETLENFSEKLEVEGRSLWQDARRRFMHNRAAVASLI
                     VLVLIALFVILAPMLSQFAYDDTDWAMMSSAPDMESGHYFGTDSSGRDLLVRVAIGGR
                     ISLMVGVAAALVAVVVGTLYGSLSGYLGGKVDSVMMRLLEILNSFPFMFFVILLVTFF
                     GQNILLIFVAIGMVSWLDMARIVRGQTLSLKRKEFIEAAQVGGVSTSGIVIRHIVPNV
                     LGVVVVYASLLVPSMILFESFLSFLGLGTQEPLSSWGALLSDGANSMEVSPWLLLFPA
                     GFLVVTLFCFNFIGDGLRDALDPKDR"
     gene            3777..4790
                     /gene="oppD"
                     /locus_tag="ACHOSC_RS17210"
                     /old_locus_tag="pgaptmp_003442"
     CDS             3777..4790
                     /gene="oppD"
                     /locus_tag="ACHOSC_RS17210"
                     /old_locus_tag="pgaptmp_003442"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein tripeptide/oligopeptide ABC transporter
                     ATP-binding protein OppD"
                     /protein_id="HGU4549326.1"
                     /translation="MSVIETATVPLAQQQADALLNVKDLRVTFSTPDGDVTAVNDLNF
                     SLRAGETLGIVGESGSGKSQTAFALMGLLAANGRIGGSATFNGREILNLPEHELNKLR
                     AEQISMIFQDPMTSLNPYMRVGEQLMEVLMLHKNMSKAEAFEESVRMLDAVKMPEARK
                     RMKMYPHEFSGGMRQRVMIAMALLCRPKLLIADEPTTALDVTVQAQIMTLLNELKREF
                     NTAIIMITHDLGVVAGICDKVLVMYAGRTMEYGNARDVFYQPVHPYSIGLLNAVPRLD
                     AEGETMLTIPGNPPNLLRLPKGCPFQPRCPHAMEICSSAPPLEEFTPGRLRACFKPVE
                     ELL"
     gene            4787..5791
                     /gene="oppF"
                     /locus_tag="ACHOSC_RS17215"
                     /old_locus_tag="pgaptmp_003443"
     CDS             4787..5791
                     /gene="oppF"
                     /locus_tag="ACHOSC_RS17215"
                     /old_locus_tag="pgaptmp_003443"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein tripeptide/oligopeptide ABC transporter
                     ATP-binding protein OppF"
                     /protein_id="HGU4549327.1"
                     /translation="MNAVTEGRKVLLEIADLKVHFEIKDGKQWFWQPPKTLKAVDGVT
                     LRLYEGETLGVVGESGCGKSTFARAIIGLVKATDGHVAWLGKELLGMKPDEWRAVRSD
                     IQMIFQDPLASLNPRMTIGEIIAEPLRTYHPKMSRQEVRERVKAMMLKVGLLPNLINR
                     YPHEFSGGQCQRIGIARALILEPKLIICDEPVSALDVSIQAQVVNLLQQLQREMGLSL
                     IFIAHDLAVVKHISDRVLVMYLGHAVELGTYDEVYHNPLHPYTRALMSAVPIPDPDLE
                     KNKTIQLLEGELPSPINPPSGCVFRTRCPIAGPECAKTRPVLEGSFRHAVSCLKVDPL
                     "
     gene            complement(5844..6173)
                     /locus_tag="ACHOSC_RS17220"
                     /old_locus_tag="pgaptmp_003444"
     CDS             complement(5844..6173)
                     /locus_tag="ACHOSC_RS17220"
                     /old_locus_tag="pgaptmp_003444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309775.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HI1450 family dsDNA-mimic protein"
                     /protein_id="HGU4549328.1"
                     /translation="MDMDLNNRLTEDETLEQAYDIFLELAADNLDPADVLLFNLQFEE
                     RGGAELFDPAEDWQEHVDFDLNPDFFAEVVIGLADSEDGEINDVFARILLCREKDHKL
                     CHIIWRE"
     gene            complement(6208..7668)
                     /gene="cls"
                     /locus_tag="ACHOSC_RS17225"
                     /old_locus_tag="pgaptmp_003445"
     CDS             complement(6208..7668)
                     /gene="cls"
                     /locus_tag="ACHOSC_RS17225"
                     /old_locus_tag="pgaptmp_003445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008808 - cardiolipin synthase activity
                     [Evidence IEA];
                     GO_function: GO:0016780 - phosphotransferase activity, for
                     other substituted phosphate groups [Evidence IEA];
                     GO_process: GO:0032049 - cardiolipin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cardiolipin synthase"
                     /protein_id="HGU4549329.1"
                     /translation="MTTVYTLVSWLAILGYWLLIAGVTLRILMKRRAVPSAMAWLLII
                     YILPLVGIIAYLAVGELHLGKRRAERARAMWPSTAKWLNDLKACKHIFAEENSSVAAP
                     LFKLCERRQGIAGVKGNQLQLMTESDDVMQALIRDIQLARHNIEMVFYIWQPGGMADQ
                     VAESLMAAARRGIHCRLMLDSAGSVAFFRSPWPKLMRNAGIEVVEALKVNLMRVFLRR
                     MDLRQHRKMIMIDNYIAYTGSMNMVDPRYFKQDAGVGQWIDLMARMEGPIATAMGIIY
                     SCDWEIETGKRILPPPPDVNIMPFEQASGHTIHTIASGPGFPEDLIHQALLTAAYSAR
                     EYLIMTTPYFVPSDDLLHAICTAAQRGVDVSIILPRKNDSMLVGWASRAFFTELLAAG
                     VKIYQFEGGLLHTKSVLVDGELSLVGTVNLDMRSLWLNFEITLAIDDKGFGADLAAVQ
                     DDYISRSRLLDARLWLKRPLWQRVAERLFYFFSPLL"
     gene            7811..7984
                     /locus_tag="ACHOSC_RS17230"
                     /old_locus_tag="pgaptmp_003446"
     CDS             7811..7984
                     /locus_tag="ACHOSC_RS17230"
                     /old_locus_tag="pgaptmp_003446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YciY family protein"
                     /protein_id="HGU4549330.1"
                     /translation="MKRSRTEVGRWRMQRQASRRKSRWLEGQSRRNMRIHSIRKCILN
                     KQRNSLLFAIYNI"
     gene            complement(8039..>9229)
                     /gene="kch"
                     /locus_tag="ACHOSC_RS17235"
                     /old_locus_tag="pgaptmp_003447"
     CDS             complement(8039..>9229)
                     /gene="kch"
                     /locus_tag="ACHOSC_RS17235"
                     /old_locus_tag="pgaptmp_003447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="voltage-gated potassium channel protein"
                     /protein_id="HGU4549331.1"
                     /translation="ILALAVFLNGLLIFKTIYGMSVNLLDIFHIKAFSELDLSLLANA
                     PLFMLGVFLVLNSIGLLFRAKLAWAISIILLLIALIYTLHFYPWLKFSIGFCIFTLVF
                     LLILRKDFSHSSAAAGTIFAFISFTTLLFYSTYGALYLSEGFNPRIESLMTAFYFSIE
                     TMSTVGYGDIVPVSESARLFTISVIISGITVFATSMTSIFGPLIRGGFNKLVKGNNHT
                     MHRKDHFIVCGHSILAINTILQLNQRGQNVTVISNLPEDDIKQLEQRLGDNADVIPGD
                     SNDSSVLKKAGIDRCRAILALSDNDADNAFVVLSAKDMSSDVKTVLAVSDSKNLNKIK
                     MVHPDIILSPQLFGSEILARVLNGEEINNDMLVSMLLNSGHGIFSDNDEQETKADSKE
                     SVQK"
BASE COUNT         2390 a         2180 c         2308 g         2351 t
ORIGIN      
        1 ataatgtgtc tcgacagggg agacacagta cgaatcgaca taaggtgatc gtctgaatca
       61 ccagaataaa taaagtcggt gatagtaata cgtaacgata aagtaacctg acagcagaaa
      121 gtctccgagc ctgtgcaggg tcccaatccg ggattacaca tgctggttaa taccagtaat
      181 tataatgagg gagtccaaaa aacaatgacc aacatcacca agagaagttt agtagcagct
      241 ggcgttctgg ctgcgctaat ggcagggaat gtcgcgctgg cagctgatgt acccgcaggc
      301 gtcacactgg cggaaaaaca aacactggta cgtaacaatg gttcagaagt tcagtcatta
      361 gatccgcaca aaattgaagg tgttccggag tctaatatca gccgagacct gtttgaaggc
      421 ttactggtca gcgatcttga cggtcatcca gcacctggcg tcgctgaatc ctgggataat
      481 aaagacgcga aagtctggac cttccatttg cgtaaagatg cgaaatggtc tgatggcacg
      541 ccagtcacag cacaagactt tgtgtatagc tggcaacgtt ctgttgatcc gaacactgct
      601 tctccgtatg ccagttatct gcaatatggg catatcgccg gtattgatga aattcttgaa
      661 gggaaaaaac cgattaccga tctcggcgtg aaagctattg atgatcacac attagaagtc
      721 accttaagtg aacccgttcc gtacttctat aaattacttg ttcacccatc aacttcaccg
      781 gtgccaaaag ccgctatcga gaaattcggc gaaaaatgga cccagcctgg taatatcgtc
      841 accaacggtg cctatacctt aaaagattgg gtcgtaaacg aacgaatcgt tcttgaacgc
      901 agcccgacct actggaacaa cgcgaaaacc gttattaacc aggtaaccta tttgcctatt
      961 gcttctgaag ttaccgatgt caaccgctac cgtagtggtg aaatcgacat gacttataac
     1021 aacatgccga tcgaattgtt ccagaagctg aaaaaagaga tcccggacga agttcacgtt
     1081 gatccatacc tgtgcactta ctattacgaa attaacaacc agaaaccgcc attcaacgat
     1141 gtgcgtgtgc gtaccgcact gaaactaggt atggaccgcg atatcattgt taataaagtg
     1201 aaagcgcagg gcgacatgcc cgcctatggt tacactccac cgtatactga tggcgcaaaa
     1261 ttgactcagc cggaatggtt tggctggagc caggaaaaac gtaacgaaga agcgaaaaaa
     1321 ctgctggctg aagcgggtta taccgcagac aaaccgttga ccatcaacct gttgtataac
     1381 acctccgatc tgcataaaaa gctggcgatt gctgcctctt cattgtggaa gaaaaacatt
     1441 ggtgtaaacg tcaaactggt taaccaggag tggaaaacgt tcctcgacac ccgtcaccag
     1501 ggtacttttg atgtggcccg tgcaggctgg tgtgctgact acaacgaacc aacttccttc
     1561 ctgaacacca tgctttcgaa cagctcgatg aataccgcgc attataagag cccggccttt
     1621 gacagcatta tggcggaaac gctgaaagtg actgacgagg cgcagcgcac agctctgtac
     1681 actaaagcag aacaacagct ggataaggat tcggccattg ttcctgttta ttactacgtg
     1741 aatgcgcgtc tggtgaaacc gtgggttggt ggctataccg gcaaagatcc gctggataat
     1801 acctataccc ggaatatgta cattgtgaag cactaatggc aatacgtggg gcaggagtgt
     1861 cctgctccac ggtgtctgat ttttatcgca ttacagaagg cacaggccag aaggtagggc
     1921 aatgttaaaa tttattctac gtcgctgtct ggaagcgatt ccgacgctat ttattcttat
     1981 tactatttcg ttctttatga tgcgcctcgc gccgggaagc ccttttaccg gcgaacgtac
     2041 tttaccgcca gaagtgatgg ccaatatcga agagaaatat catcttaatg atccgatcat
     2101 gacacagtat ttcagctacc tgaaacaact ggcgcacggt gatttcggtc catcgtttaa
     2161 atataaagat tattcggtca atgacttggt tgcatccagt tttcccgttt ctgccaaact
     2221 gggagccgca gcatttttcc ttgcggtaat actgggggtt agtgctggcg ttattgccgc
     2281 attaaaacaa aacaccaaat gggactatac cgtgatgggg ctggcaatga ccggggttgt
     2341 tatccccagt tttgtggttg caccattatt agtcatgata tttgcgatca ttttgcattg
     2401 gctgccgggc ggtggctgga atggtggggc gcttaaattc atgatattgc cgatggtggc
     2461 gttgtcactc gcttatatcg ccagtattgc gcgtattacc cgtggctcta tgattgaagt
     2521 attacactcc aattttattc gtactgcccg ggcgaaaggg ttacctatgc ggcggatcat
     2581 tttacgtcac gcattaaaac ctgctctgtt acccgtgctc tcttatatgg gccccgcatt
     2641 tgtcggcatt attaccggtt ctatggttat cgaaaccatt tatggtttgc cggggattgg
     2701 acaactgttc gttaatggcg cattgaaccg tgactattcc ttagtgttaa gcctgaccat
     2761 cctggttggt gctttaacca ttttgtttaa tgccattgtc gatgtgctat atgcggttat
     2821 cgacccgaaa atccgttact gatactggag ctcgcgatga tgttaagtaa gaaaaacagc
     2881 gagacgctgg aaaatttcag tgaaaagctg gaggtcgaag ggcgcagctt gtggcaggac
     2941 gcacgtcgac gttttatgca taaccgtgcg gcggttgcca gtctgatagt gctggtgctg
     3001 atcgcgttat ttgtaatcct ggcaccgatg ctttcccagt ttgcctatga cgatactgac
     3061 tgggcgatga tgtccagcgc cccggatatg gagtccggtc actactttgg tactgactca
     3121 tccggtcgcg acctgcttgt gcgcgttgcg attggcgggc gtatctcact catggtcggt
     3181 gttgctgcgg cactggtggc tgtggtcgtc gggacacttt atggttcgct ttccggttat
     3241 ctgggcggta aagtggattc ggtaatgatg cgtctgctgg aaatcctcaa ctccttccca
     3301 ttcatgttct tcgtcatttt gctggtgacc tttttcggtc aaaacatcct gctgattttc
     3361 gtggcgatag gcatggtttc ctggctggat atggctcgta ttgtgcgtgg gcaaaccctg
     3421 agtctgaagc gcaaagagtt tattgaggcg gcacaagttg gcggtgtatc gacatcgggc
     3481 attgttattc gccacattgt gccgaatgta ctcggtgtgg tggtggtcta cgcatcactt
     3541 ctggtgccca gcatgatcct ctttgaatct ttccttagct tcctggggtt gggtacgcaa
     3601 gagccgttaa gcagctgggg tgcattgctg agtgatggcg cgaactcgat ggaagtctct
     3661 ccatggttat tgttgttccc agcgggattc ctcgtggtga cgctgttttg tttcaacttt
     3721 atcggcgatg gcttgcgtga tgccctcgac ccgaaagatc gttaaggagt gcagccatga
     3781 gcgtaattga aactgcaact gtgccgctcg cacaacaaca ggctgacgca ctgctgaacg
     3841 tgaaagattt gcgtgtcacc tttagtaccc cggacggcga cgtcacggcg gtcaatgatt
     3901 tgaatttttc cctacgtgcc ggagaaacgc tgggtattgt aggtgagtct ggttcgggta
     3961 aatcgcaaac tgcatttgcg ttgatgggcc tgttggctgc caacggacgt attggcggat
     4021 cggcaacctt caatgggcgt gaaatcctca atttgccaga gcatgaactc aataaactgc
     4081 gcgctgaaca aatctcaatg atttttcagg acccaatgac ttcgttgaat ccctatatgc
     4141 gcgtcggtga gcagttaatg gaagtgctga tgctgcataa gaacatgagc aaagctgagg
     4201 cgtttgaaga gtcggtgcgg atgctcgatg cggtaaaaat gccggaagcg cgtaaacgca
     4261 tgaaaatgta cccgcacgaa ttttctggcg gcatgcgtca gcgagtcatg attgcgatgg
     4321 cattgctatg tcgacctaag ctactgattg cggatgaacc cactactgcg ctggacgtca
     4381 ccgtacaggc gcagatcatg acgctattaa atgaactgaa gcgggaattt aataccgcca
     4441 tcattatgat cacccacgat cttggcgtgg tggcggggat ctgtgacaaa gtgctggtaa
     4501 tgtacgccgg gcgcacgatg gaatatggca atgcgcgcga tgtcttttat caacccgttc
     4561 atccttattc tatcggtttg ctcaacgcgg tgccgcgtct tgatgcggaa ggtgaaacaa
     4621 tgttgaccat cccgggtaat ccgccaaact tgctgcgatt accgaaaggt tgcccgttcc
     4681 agccacgttg tccacatgcg atggaaattt gtagtagcgc accgccgctg gaagagttta
     4741 cgcctggccg tctgcgtgct tgctttaaac cggtggagga actgttatga atgctgtaac
     4801 tgaaggaaga aaagtcctcc ttgaaatcgc cgatcttaaa gtgcactttg aaatcaaaga
     4861 tggcaaacag tggttctggc aaccgccgaa aacgctcaaa gccgtcgatg gtgtcactct
     4921 tcgcctgtat gaaggggaaa cattaggtgt ggtaggggaa tcgggatgcg gtaagtccac
     4981 ctttgctcgc gccatcatcg gtttggtcaa ggcgaccgac ggtcatgttg cctggttagg
     5041 taaagagttg ctgggcatga agcccgatga atggcgtgcc gttcgcagtg atattcagat
     5101 gattttccag gatccgttgg catcgctaaa cccgcgtatg accatcggcg agatcatcgc
     5161 tgaaccactg cgtacttatc atccgaaaat gtcacgccag gaagttcgcg agcgcgtgaa
     5221 ggcgatgatg ctgaaagtcg ggttattgcc taacctgatt aaccgctatc cgcatgagtt
     5281 ctccggtggg cagtgccagc gtatcgggat tgctcgtgct cttattcttg aaccgaagct
     5341 gattatctgc gatgagccgg tttcggcgct ggacgtgtca attcaggcgc aggtggtcaa
     5401 cctgctccag cagctgcaac gtgagatggg attgtcatta atttttatcg ctcatgacct
     5461 ggccgtggta aaacacattt ccgatcgtgt gttggtgatg tatctcggcc atgcggtaga
     5521 actggggacc tatgatgagg tctaccacaa tccactacat ccttacacca gggcattgat
     5581 gtcggcagtc cccatacctg atccggatct ggagaagaac aaaaccatcc agttactgga
     5641 aggggaatta ccgtcgccga tcaacccgcc ttccggttgt gttttccgta cccgttgccc
     5701 gattgccggt ccggagtgcg ccaaaacacg tcctgttctg gaggggagtt tcagacacgc
     5761 cgtttcttgc ctgaaagtcg atccgcttta aacacaataa gggctgacag ttgtcagccc
     5821 tttttcacgc taaaagcgat tatttattcc cgccagatga tatggcaaag tttgtgatct
     5881 ttttcgcggc ataacagaat gcgggcgaaa acatcgttga tttcaccatc ttcactgtcc
     5941 gccagaccaa tcaccacttc ggcaaaaaag tctgggttca aatcgaaatc aacatgttcc
     6001 tgccagtctt ccgccggatc gaataactcc gcgccgccgc gctcttcaaa ctgaagattg
     6061 aacagcagaa catcagctgg atcgagattg tcggcagcca gttcgagaaa aatatcgtag
     6121 gcctgctcaa gcgtttcatc ttcagtcagg cgattgttta gatccatatc catagtcact
     6181 acctgtttaa cctctgttgg cgacgtttta cagcaacgga ctgaagaagt aaaacagtcg
     6241 ctcggcgaca cgttgccata atggacgttt tagccataaa cgggcatcga gcagacgtga
     6301 acgcgaaata taatcgtcct gaacggcggc aaggtctgca ccaaaacctt tatcgtcgat
     6361 tgccagggta atctcgaaat tcagccacag actccgcata tcaaggttaa cggtgccaac
     6421 cagacttagt tcgccatcga ccagcacgct cttggtatgc agtaacccgc cttcaaactg
     6481 ataaatttta accccagcag caagcagttc cgtaaagaat gcgcgactgg cccagccgac
     6541 cagcatcgag tcattttttc gcggaaggat aatactgaca tccaccccgc gctgcgccgc
     6601 cgtgcaaatc gcatgaagta aatcatcgct tggcacaaag tagggcgtgg tcatgatcaa
     6661 atattcacgc gccgaataag ccgcagtcaa taatgcctgg tgaatgagat cttccggaaa
     6721 gccggggcca gaagcaattg tgtgaatggt gtgaccgctg gcctgttcaa acggcataat
     6781 attgacatct ggtggtggcg gcagaatacg ttttccggtt tcaatctccc agtcgcagga
     6841 ataaataatc cccatcgcgg tggcgatggg gccttccata cgcgccatca gatcaatcca
     6901 ttgccctacg cccgcatctt gtttgaagta gcgaggatcg accatattca tgctgccggt
     6961 gtacgcgatg taattatcga tcatgatcat cttgcgatgt tggcgcaggt ccatacggcg
     7021 taaaaacaca cgcatcagat tgacctttaa ggcttcgacc acttcaatac cggcattacg
     7081 cattagcttg ggccacgggc tgcggaaaaa agccacactc ccggcggagt cgagcatcaa
     7141 tcggcaatga ataccacgcc gtgcagccgc cattaatgat tcagccacct ggtccgccat
     7201 gccgccgggc tgccagatat aaaacaccat ctcaatatta tggcgcgcga gctggatgtc
     7261 gcggattaac gcctgcatca catcatctga ctcggtcatc agttgtagct gattcccttt
     7321 gaccccagcg atcccctgac gacgctcgca aagcttgaat aatggcgcag cgacactgct
     7381 attttcttcg gcgaagatat gcttacaggc tttaaggtcg ttaagccatt ttgcggtgga
     7441 aggccacatc gctctggcgc gctcagcgcg gcgtttgcct aaatggagct cgccaacggc
     7501 aagataggca ataattccga ctaacggcag aatgtaaata atcaacagcc aggccatcgc
     7561 ggagggaact gcgcgtcgtt tcattagaat gcgtaaagtt acgcctgcaa tgagcaacca
     7621 gtatcccaga atggccaacc aactcaccaa cgtataaacg gttgtcatag attagaaatc
     7681 cttttgaaag cgcattgtta tgagtttacg catatggatt catctggcaa ataaaaacgc
     7741 gggaaaagca ctggtcagcg tcgggggtgg gggtataatg accattctgt tattgcatag
     7801 agtagttaac atgaagcgga gtagaacgga agtggggcgc tggcgcatgc agcgtcaggc
     7861 tagccgacgt aaatcgcgtt ggcttgaggg gcaatcgcgc cgaaatatgc gtatccacag
     7921 catcaggaag tgcattctaa acaaacagcg taactcgtta ttgtttgcga tctacaatat
     7981 ctaaatatac tgggcaccat tgcggtgccc aataagttta ctctttcgtc tttaaactct
     8041 atttttgcac tgattcttta ctgtcagctt tcgtttcttg ttcatcgtta tcgctgaaaa
     8101 tgccatgacc ggagttcaac aacattgaaa cgagcatatc attattaatc tcttcaccat
     8161 ttaaaactcg cgccagaatt tcgctgccaa acagttgcgg tgagagaatg atatccggat
     8221 gtaccatctt aatcttattc aggtttttgc tatcactgac ggcgagaact gttttgacat
     8281 cactgctcat atcttttgcc gagagtacaa caaacgcgtt atctgcatcg ttatcactca
     8341 gcgccagaat ggcccggcaa cgatcgattc ccgctttctt taatactgaa ctgtcattac
     8401 tgtcaccggg gataacatca gcgttatcgc ctaagcgttg ctcaagttgc ttgatatcat
     8461 cttcaggcaa gttgctgata accgttacgt tttgtccgcg ttgattcagt tgcaggatcg
     8521 tattgatggc gagaatcgaa tgtccgcaaa caataaaatg atctttacga tgcattgtat
     8581 gattgtttcc ttttacaagt ttgttgaatc ccccgcggat aagcggacca aaaattgagg
     8641 tcatagatgt ggcaaaaacg gtaatgccgg aaataatgac cgaaatagtg aacaatcgtg
     8701 ctgattcaga aacagggaca atatcgccat agccgacggt tgacatggtt tctatcgaaa
     8761 aatagaacgc ggtcatcaaa ctttctattc gcggattaaa accttcgctt aaataaagcg
     8821 caccgtaggt tgagtaaaac agtaatgtcg tgaaactaat aaatgcaaaa attgtcccgg
     8881 ctgcggcgct actgtgggag aagtctttgc gcagtatcag caaaaacacc agcgtaaaaa
     8941 tgcaaaatcc aatactaaat ttcagccagg gataaaaatg cagggtgtaa attagcgcta
     9001 tcaacaacaa aatgatactg attgcccatg cgagctttgc ccggaacagt aagccaatgg
     9061 agttcaggac aagaaagacg ccgagcataa atagtggggc gtttgccagc aaggagagat
     9121 ccaactctga aaatgctttg atatggaaaa tatcaagcaa attgaccgac ataccataga
     9181 ttgttttaaa aataagcaat ccatttaaaa agacggccag cgcgagaat
//
LOCUS       DAZKHF020000179         9180 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2201S31241
            SAMN43267800-rid25106413.denovo.180, whole genome shotgun sequence.
ACCESSION   DAZKHF020000179 DAZKHF020000000
VERSION     DAZKHF020000179.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267800
            Sequence Read Archive: SRR30307263
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 9180)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9180)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 9180)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350581.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350581.2
            Genome Coverage       :: 151x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:21:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,652
            CDSs (total)                      :: 5,557
            Genes (coding)                    :: 5,320
            CDSs (with protein)               :: 5,320
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 237
            CDSs (without protein)            :: 237
            Pseudo Genes (ambiguous residues) :: 0 of 237
            Pseudo Genes (frameshifted)       :: 102 of 237
            Pseudo Genes (incomplete)         :: 130 of 237
            Pseudo Genes (internal stop)      :: 52 of 237
            Pseudo Genes (multiple problems)  :: 41 of 237
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9180
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267800-rid25106413.denovo.180"
                     /serotype="O 26:H11"
                     /isolate="2201S31241"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-01"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            complement(<1..582)
                     /locus_tag="ACHOSC_RS17240"
                     /old_locus_tag="pgaptmp_003448"
     CDS             complement(<1..582)
                     /locus_tag="ACHOSC_RS17240"
                     /old_locus_tag="pgaptmp_003448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001215756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1175 family protein"
                     /protein_id="HGU4549332.1"
                     /translation="MRHGLLALICWLCCVVAHSEMLNVEQSGLFRAWFVRIAQEQLRQ
                     GPSPRWYQQDCAGLVRFAANETLKVHDSKWLKSNGFSSQYLPPEMTLTPGQRQLAQNW
                     NQGNGKTGPYVTAINLIQYNSQFIGQDINQALPGDMIFFDQGDAQHLMVWMGRYVIYH
                     TGSATKTDNGMRAVSLQQLMTWKLYIKISLIINM"
     gene            complement(579..2267)
                     /gene="yfaA"
                     /locus_tag="ACHOSC_RS17245"
                     /old_locus_tag="pgaptmp_003449"
     CDS             complement(579..2267)
                     /gene="yfaA"
                     /locus_tag="ACHOSC_RS17245"
                     /old_locus_tag="pgaptmp_003449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416733.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2138 domain-containing protein"
                     /protein_id="HGU4549333.1"
                     /translation="MSGEKKAKGWRFYGLVGFGAIALLSAGVWALQYAGSGPEKTLSP
                     LVVHNNLQIDLNEPDLFLDSDSLSQLPKDLLTIPFLHDVLSEDFVFYYQNHADRLGIE
                     GSIRRIVYEHDLTLKDKLFSSLLDQPAQAALWHDKQGHLSHYMVLIQRSGLSKLLEPL
                     LFAATSDSQLSKTEISSIKINSETVPVYQLRYNGNNALMFATYQDKMLVFSSTDMLFK
                     DDQQDTEATAIAGDLLSGKKRWQASFGLEERTAEKTPVRQRIVVSARLLGFGYQRLMP
                     SFAGVRFEMGNDGWHSFVALNDESASVDTSFDFTPVWNSMPAGASFCVVVPYSHGIAE
                     EMLSHISQENDKLNGALDGAAGLCWYADSKLQTPLFVGQFDGTAEQAQLPGKLFTQNI
                     GAHESKAPEGVLPISQTQQGEAQIWRREVSSRYGQYPKAQAAQPDQLMSDYFFRVSLA
                     MQNKTLLFSLDDTLVNNALQTLNKNRPAMVDVIPTDGIVPLYINPQGVAKLLRNETLT
                     SLPKNLEPVFYNAAQTLLMPKLDALSQQPRYVMKLAQMEPGAAWQWLPITWQPL"
     gene            complement(2416..5043)
                     /gene="gyrA"
                     /locus_tag="ACHOSC_RS17250"
                     /old_locus_tag="pgaptmp_003450"
     CDS             complement(2416..5043)
                     /gene="gyrA"
                     /locus_tag="ACHOSC_RS17250"
                     /old_locus_tag="pgaptmp_003450"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009330 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) complex [Evidence
                     IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase (ATP-hydrolyzing) subunit A"
                     /protein_id="HGU4549334.1"
                     /translation="MSDLAREITPVNIEEELKSSYLDYAMSVIVGRALPDVRDGLKPV
                     HRRVLYAMNVLGNDWNKAYKKSARVVGDVIGKYHPHGDSAVYDTIVRMAQPFSLRYML
                     VDGQGNFGSIDGDSAAAMRYTEIRLAKIAHELMADLEKETVDFVDNYDGTEKIPDVMP
                     TKIPNLLVNGSSGIAVGMATNIPPHNLTEVINGCLAYIDDEDISIEGLMEHIPGPDFP
                     TAAIINGRRGIEEAYRTGRGKVYIRARAEVEVDAKTGRETIIVHEIPYQVNKARLIEK
                     IAELVKEKRVEGISALRDESDKDGMRIVIEVKRDAVGEVVLNNLYSQTQLQVSFGINM
                     VALHHGQPKIMNLKDIIAAFVRHRREVVTRRTIFELRKARDRAHILEALAVALANIDP
                     IIELIRHAPTPAEAKTALVANPWQLGNVAAMLERAGDDAARPEWLEPEFGVRDGLYYL
                     TEQQAQAILDLRLQKLTGLEHEKLLDEYKELLDQIAELLRILGSADRLMEVIREELEL
                     VREQFGDKRRTEITANSADINLEDLITQEDVVVTLSHQGYVKYQPLSEYEAQRRGGKG
                     KSAARIKEEDFIDRLLVANTHDHILCFSSRGRVYSMKVYQLPEATRGARGRPIVNLLP
                     LEQDERITAILPVTEFEEGVKVFMATANGTVKKTVLTEFNRLRTAGKVAIKLVDGDEL
                     IGVDLTSGEDEVMLFSAEGKVVRFKESSVRAMGCNTTGVRGIRLGEGDKVVSLIVPRG
                     DGAILTATQNGYGKRTAVAEYPTKSRATKGVISIKVTERNGLVVGAVQVDDCDQIMMI
                     TDAGTLVRTRVSEISIVGRNTQGVILIRTAEDENVVGLQRVAEPVDEEDLDTIDGSAA
                     EGDDEIAPEVDVDDEPEEE"
     gene            5190..5912
                     /gene="ubiG"
                     /locus_tag="ACHOSC_RS17255"
                     /old_locus_tag="pgaptmp_003451"
     CDS             5190..5912
                     /gene="ubiG"
                     /locus_tag="ACHOSC_RS17255"
                     /old_locus_tag="pgaptmp_003451"
                     /EC_number="2.1.1.222"
                     /EC_number="2.1.1.64"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0061542 - 3-demethylubiquinol-n
                     3-O-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006744 - ubiquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 2-polyprenyl-6-hydroxyphenol
                     methylase/3-demethylubiquinol 3-O-methyltransferase UbiG"
                     /protein_id="HGU4549335.1"
                     /translation="MNAEKSPENHNVDHEEIAKFEAVASRWWDLEGEFKPLHRINPLR
                     LGYIAERAGGLFGKKVLDVGCGGGILAESMAREGATVTGLDMGFEPLQVAKLHALESG
                     IQVDYVQETVEEHAAKHAGQYDVVTCMEMLEHVPDPQSVVRACAQLVKPGGDVFFSTL
                     NRNGKSWLMAVVGAEYILRMVPKGTHDVKKFIKPAELLGWVDQTSLKERHITGLHYNP
                     LTNTFKLGPGVDVNYMLHTQNK"
     gene            complement(6040..>9180)
                     /gene="yfaL"
                     /locus_tag="ACHOSC_RS17260"
                     /old_locus_tag="pgaptmp_003452"
     CDS             complement(6040..>9180)
                     /gene="yfaL"
                     /locus_tag="ACHOSC_RS17260"
                     /old_locus_tag="pgaptmp_003452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AIDA-I family autotransporter adhesin YfaL/EhaC"
                     /protein_id="HGU4549336.1"
                     /translation="SYSDGDGGAIDVTDNNSDSKHPSGYTIINNTAFTNNTAEDYGGA
                     IYTNSATAPYLIDISVDDSYSQNGGVLVDENNSAAGYGDGPSTAAGGFMYLGLSEVTF
                     DIADGKTLVIGNTENDGAVDSIAGTGLITKTGSGDLVLNADNNDFTGEMQIENGEVTL
                     GRSNSLMNVGDTHCQDDPQDCYGLTIGSIDKYQNQAELNVGSTQQTFVHSLTGFQNGT
                     LNIDAGGNVTVNQGSFAGTIEGAGQLTIAQNGSYVLSGAQSMALTGDIVVDDGAVLSL
                     EGDAADLTALQDDPQSIVLNGGVLDLSDFSTWQSGTSYNDGLEVSGSSGTVIGSQDVV
                     DLAGGNDMHIGGDGKDGVYVVVDAGDGQVSLANNNSYLGTTQIASGMLEVSDNSQLGD
                     THYNRQVIFTDKQQESVMEITANVDTRSTTTEHGRDIEMRADGEVAVDAGVDTQWGAL
                     MADSSGQHQDEGSTLTKTGAGTLELTASGTTQSAVRVEEGTLKGDVADILPYASSLWV
                     GDGATFVTGADQDIQSIDATSSGTIDISDGTVLRLTGQDTSVALNASLFNGDGTLVNA
                     TDGVTLTGELNTNLETDSLTYLSDVTVNGNLTNTSGAVSLQNGVAGDTLTVNGDYTGG
                     GTLLLDSELNGDDSVSDQLVLNGNTAGNTTVVVNSITGIGEPTSTGIKVVDFAADPTQ
                     FQNNAKFSLAGSGYVNMGAYDYTLVEDNNDWYLRSQEVTPPLPPDPDPDPTPDPDPTP
                     DPIPAYQPVLNAKVGGYLNNLRAANQAFMMERRDHAGGNGQTLNLRVIGGRYHYTAAG
                     QLAQHEDTSTVQLSGDLFSGRWGDDGEWMLGAVGGYSDNQGESRSNMTGTRADNQNHG
                     YAVGLTSSWYQHGNQKQGAWLDSWLQYAWFNNDVSEQDDGTDHYHSSGIIASLEAGYQ
                     WLPGRGVVIEPQAQVIYQGVQQDDFTAANHARVSQSQGDDIQTRLGLHSEWRTAVGVT
                     PTLDLNYYHDPHATEIEEDGSTISDDAVKRRGEIKVGVTGNISQRVSLRGSVAWQKGS
                     DDFAQTAGFLSMTVKW"
BASE COUNT         2096 a         2585 c         2247 g         2252 t
ORIGIN      
        1 catattgata attaatgaaa ttttaatata caacttccat gtcataagtt gttgcagact
       61 gactgcgcgc attccgttgt cagttttcgt ggcgcttccg gtgtggtaga tgacgtaacg
      121 ccccatccag accattaagt gctgggcatc gccctgatcg aaaaaaatca tatcgccagg
      181 cagcgcctgg tttatgtcct ggccaataaa ctggctgttg tactgaatca aattaatcgc
      241 ggtcacgtag gggccggttt tcccgttccc ctgattccag ttttgcgcca gttgacgctg
      301 tccaggtgtt agcgtcatct ctggcggcaa atactggcta gagaaaccgt tactttttag
      361 ccatttacta tcgtgaactt tcagcgtctc gttcgccgca aatcgcacca ggcccgcaca
      421 atcctgctga taccagcgtg gacttggccc ctggcggagc tgttcttgtg caatgcgtac
      481 aaaccaggcg cgaaacagcc cggattgttc gacattcagc atttcgctat gggcaacaac
      541 acaacacagc cagcaaatca gcgccagcag cccgtgcctc atagcggctg ccaggttatg
      601 ggtagccact gccaggcggc accgggttcc atctgggcca gcttcatgac ataacgcggt
      661 tgttgagata aagcgtccag cttcggcatt aataaagttt gtgcggcgtt ataaaaaacc
      721 ggttcgagat tcttcggcag actggtcaac gtttcgttac gcagcagttt cgccacgcct
      781 tgtggattga tatagagcgg aacgatgcca tcagtgggta ttacatccac cattgccggg
      841 cggtttttat tcagtgtttg cagggcgtta ttaaccagcg tgtcatcgag ggagaaaagc
      901 agcgttttgt tttgcatcgc cagcgacacg cggaaaaaat aatccgacat taattgatcg
      961 ggttgcgccg cctgcgcttt cggatactga ccgtatcggg aactcacttc gcgacgccag
     1021 atttgcgctt cgccctgctg agtctggctt atcggcaaaa caccttctgg cgctttgctt
     1081 tcgtgcgcac caatattttg cgtaaacagt ttccctggca attgcgcctg ttcggcagtg
     1141 ccatcaaact gaccgacaaa cagcggggtt tgcaattttg agtctgcata ccagcacagc
     1201 cccgcggcac cgtctaacgc cccattcaac ttgtcgtttt cctgactgat gtgcgaaagc
     1261 atctcttcgg caataccgtg tgaatacggc accaccacac agaagctggc cccggcaggc
     1321 atactgttcc agaccggcgt aaaatcgaaa ctggtatcta cgctggcgga ttcatcattt
     1381 aacgccacaa aactgtgcca gccgtcgtta cccatttcga agcgtacgcc agcaaaagaa
     1441 ggcattaacc gctggtagcc aaaacccagc aacctggcgc tgaccacgat gcgctggcgt
     1501 actggcgttt tttcagcagt acgctcttcc aggccaaagc ttgcttgcca gcgttttttg
     1561 ccgctcaaca aatcacctgc gatcgccgtg gcttcggtat cctgctgatc atctttaaac
     1621 aacatatccg tgctggaaaa caccagcatc ttgtcctgat aagtcgcgaa catcagggcg
     1681 ttattgccgt tatagcgcaa ctgataaaca ggaacagttt cactatttat cttgatgcta
     1741 ctgatttccg ttttgcttaa ctggctgtcg ctggtagcgg caaacaacaa tggctccagc
     1801 agtttgctta aaccactgcg ctggatcagc accatataat gtgaaagatg gccttgttta
     1861 tcgtgccaca gcgctgcctg cgcgggctga tctaagagtg acgaaaagag cttatctttc
     1921 agcgtgagat cgtgttcata gacaatacga cgaatgctgc cttcaatgcc cagacgatcg
     1981 gcatgattct gataatagaa aacgaaatct tcgctcagaa catcgtggag aaacggaatg
     2041 gtgaggagat ctttgggaag ctggctcaga gagtcgctgt cgagaaagag gtccggctca
     2101 ttgagatcga tttgcagatt gttgtgcacc accagcggcg acaacgtttt ttctggccca
     2161 ctgccagcat attgcaacgc ccagacgcca gcggaaagca gtgctattgc gccaaaacct
     2221 acaagaccat agaaccgcca gcctttcgcc tttttttcac cactcattgc cacattcctt
     2281 gtgtatagcc agccattttt tacgggcaca gccaaacttt accgtgccct aatacgacaa
     2341 aagcccagac tttgcagcct ggacttttca attcaaacaa gggagatagc tcccttttgg
     2401 catgaagaag taaaattatt cttcttctgg ctcgtcgtca acgtccactt ccggagcgat
     2461 ttcatcgtcc ccttccgcgg cactgccatc gatggtatcc agatcttcct cgtcaaccgg
     2521 ttcagcaaca cgttgcagac ccactacgtt ttcatcttcc gcagtacgga tgaggatcac
     2581 gccctgggtg ttacggccta cgatgctgat ttccgaaacg cgagtacgta ccagcgtacc
     2641 ggcatcggtg atcatcatga tctggtcgca gtcatctacc tgtaccgcgc caaccactaa
     2701 accattacgt tcagtaacct taatggagat aacccctttc gtcgcacgcg acttggttgg
     2761 gtattccgcc actgcggtac gtttaccgta accgttttgc gttgcggtga ggattgcgcc
     2821 atcgccacga ggcacgatca gagagacgac tttatcgcct tcacctaagc gaataccgcg
     2881 aacaccggtg gtgttgcagc ccatcgcacg gacagaagac tctttaaagc gcaccacttt
     2941 accttcagcg gagaacagca ttacttcgtc ttcgccgctg gtcaggtcaa cgccgatcag
     3001 ctcatcgccg tcaaccagtt tgatcgccac tttaccggcg gtacgcagac ggttgaactc
     3061 ggtgaggaca gttttcttca cggtaccgtt agcggtcgcc atgaagactt tcacgccttc
     3121 ttcaaactcg gtcactggca ggatcgcagt gatacgttcg tcctgctcca gcggcagcag
     3181 gttgacgatc ggacgaccgc gcgcgccacg agtggcttcc ggcaactgat aaactttcat
     3241 cgaatagacg cgaccacggc tggagaagca cagaatatgg tcgtgagtgt tcgccaccag
     3301 cagtcggtcg ataaagtctt cttctttaat acgtgcggca gatttacctt tcccgccacg
     3361 acgctgcgct tcgtattcag aaagcggctg atacttaacg tagccctggt gagagagcgt
     3421 cactaccaca tcttcctgag tgatcagatc ttccaggttg atgtctgcgc tgttggcggt
     3481 gatttcagta cgacgtttgt caccgaactg ttcacgaacc agctccagct cttcacggat
     3541 cacttccatc aaacgatcgg cgctaccaag aatacgcaac agttccgcga tctgatccag
     3601 cagctctttg tattcgtcga gcagtttttc gtgctcaagg ccggtcagtt tctgcaaacg
     3661 cagatccaga atcgcctgag cttgctgttc ggtcaggtag tacagaccat cacgcacgcc
     3721 gaactctggc tccagccatt ccggacgcgc agcatcgtcg ccagcacgtt cgagcatcgc
     3781 ggcaacgttg cccagctgcc acggattagc aaccagcgca gttttcgctt ctgcaggcgt
     3841 cggcgcatga cggatcagtt cgatgatcgg gtcgatgttc gccagcgcca cggctaatgc
     3901 ttcaaggata tgagcacgat cgcgagcttt acgcagttcg aaaatagtac gacgggtcac
     3961 cacttcacgg cggtgacgaa caaacgccgc gatgatgtct ttcaggttca tgatcttcgg
     4021 ctgaccatgg tgcaatgcca ccatgttgat accgaaagaa acctgcaact gggtctggga
     4081 gtagaggttg ttgagcacaa cttcaccgac cgcatcgcgt ttcacttcaa tcacgatgcg
     4141 cataccgtct ttgtcagact cgtcacgcag cgcgctgatg ccttccacgc gtttttcttt
     4201 taccagttcc gcaatcttct cgatcaggcg cgctttgttt acctgatacg gaatttcgtg
     4261 gacgataatg gtttcacgac cggttttggc gtcaacttcc acttctgcgc gagcgcggat
     4321 atacaccttg ccgcgaccgg tacggtaagc ttcttcaata ccgcgacgac cgttaatgat
     4381 tgccgccgtc gggaagtccg gccccgggat gtgttccatc agcccttcaa tgctgatgtc
     4441 ttcatcatcg atatacgcca gacaaccgtt gatgacttcc gtcaggttgt gcggcgggat
     4501 gttggttgcc atacctacgg cgataccgga agaaccgttc accagcaggt taggaatttt
     4561 ggttggcatg acgtcgggaa ttttttccgt gccgtcatag ttatcaacga aatcgaccgt
     4621 ctctttttcg agatcggcca tcagttcatg ggcaattttc gccagacgga tttccgtata
     4681 acgcattgcc gccgcagagt cgccgtcgat ggaaccgaag ttaccctgac cgtctaccag
     4741 catgtaacgc agcgagaatg gctgcgccat acggacgatc gtgtcataaa ccgccgagtc
     4801 accatgggga tggtatttac cgattacgtc accaacgaca cgggcagatt ttttataggc
     4861 tttgttccag tcattgccta gtacgttcat ggcgtaaagt acgcgacggt gtaccggctt
     4921 caggccatct cggacatctg gcagcgcacg gccaacaatg accgacatcg cataatccag
     4981 ataggagctc ttcagctctt cctcaatgtt gaccggtgta atttctctcg caaggtcgct
     5041 catctaaccg ctatccctct actgtatccc ggattcaaag gtcgcaaatt ataacacagc
     5101 cgcgcagttt gaggtaaacc tatacgcttt attcacatcc aatgcctgat atactcgttt
     5161 gtcttgccaa ttacggagta gaagtgccaa tgaatgccga aaaatcgccg gaaaaccata
     5221 acgtagacca cgaagagatc gctaaatttg aagccgtcgc ctcccgctgg tgggatcttg
     5281 aaggtgagtt caaaccgttg caccgcatta acccgctgcg tctgggctat attgccgagc
     5341 gtgctggcgg tttatttggc aaaaaggtgc tcgatgtcgg ttgtggcggc ggcattctgg
     5401 ccgagagtat ggcgcgcgaa ggcgcgacgg tgaccggtct ggatatgggc tttgagccat
     5461 tgcaggtggc aaaactgcac gcgctggaaa gcggcattca ggtggattat gtgcaggaaa
     5521 ccgtggaaga gcacgcggca aaacatgccg ggcagtatga tgtggtgacc tgcatggaga
     5581 tgctggagca cgtccccgat ccgcagtcag tggtcagagc ctgtgcgcaa ctggtgaaac
     5641 caggcggcga tgtctttttc tcgacgctta accgcaacgg caagtcatgg ctgatggcgg
     5701 tggttggcgc ggaatatatt ttgcgcatgg tgcccaaagg cacgcatgat gtgaagaagt
     5761 ttattaaacc ggcagaactg cttggctggg tagatcagac aagccttaaa gagcgacata
     5821 taaccgggct acattacaac ccgctcacta atacttttaa actcggcccc ggcgtggatg
     5881 tgaactatat gctgcacacg cagaataagt gaggttgttg tttggccgag ccaatgcctg
     5941 atgcgacgct tgccgcgtct tatcaggcct acaaatgctc cccgtaggcc ggataaggcg
     6001 tttacgccgc atccggcaac cgtgccgact agacagtgat taccatttca ccgtcatcga
     6061 caaaaaccct gccgtctggg caaaatcatc actccctttc tgccacgcca cgctgccgcg
     6121 cagggacact cgctgactga tattgcccgt gactcccact tttatttcac cccgtcgctt
     6181 caccgcatcg tcactgatag tgctgccatc ttcttcaatt tccgtcgcat ggggatcgtg
     6241 ataataattc agatctaatg ttggtgtgac accaaccgcg gtacgccatt cgctgtgtaa
     6301 acccagccgc gtctgaatat catccccctg cgattgtgac acgcgcgcat ggttagcggc
     6361 ggtaaaatca tcctgctgca cgccctgata aatcacctgc gcctgcggtt caatcaccac
     6421 accacgcccc ggtaaccact gatacccggc ttccagcgag gcgataatcc ccgacgagtg
     6481 gtaatgatct gtgccatcgt cttgttcgga aacatcattg ttaaaccacg cgtattgcag
     6541 ccagctatcc agccaggccc cctgcttctg attaccgtgc tgataccagc ttgaggtcag
     6601 cccaacggca taaccgtggt tctggttatc ggcgcgagtt ccggtcatat tcgagcggct
     6661 ctcgccctga ttatcactgt agccaccaac cgccccaagc atccactcgc catcatcgcc
     6721 ccagcgcccg ctaaacaggt caccgctaag ctgcaccgta gaagtgtctt catgctgagc
     6781 cagttgccct gccgccgtgt aatgataacg tccgccgata acacgtaaat tcagcgtctg
     6841 accattgcca cctgcatgat cgcgtcgctc catcataaac gcctgatttg ccgcccgcag
     6901 attattgaga taaccgccaa ctttggcatt cagcaccggc tggtaagcgg ggattggatc
     6961 gggtgttggg tcaggatcgg gtgttgggtc agggtctgga tcaggtggca atggtggcgt
     7021 tacttcttgc gatcgcagat accagtcgtt gttatcttcc accagcgtgt agtcatatgc
     7081 tcccatattg acgtagccgc tgcctgccag actgaacttc gcattgtttt gaaactgcgt
     7141 gggatcggct gcgaaatcaa ccactttaat gcctgtcgat gtcggctcac caatccctgt
     7201 aatggagtta accaccacag ttgtgttgcc agcagtatta ccgttcaaca ccaattgatc
     7261 gcttaccgag tcatcgccgt ttaattcgct atcgagcagt agcgtaccgc cgccggtata
     7321 atcaccgttt accgtcagcg tatcgccagc gacgccattt tgcaggctaa ccgcaccgga
     7381 cgtattggtc agattgccat taaccgtcac gtcggaaaga taggtcaggc tgtcagtttc
     7441 aaggttggta ttaagctcac ctgtcaacgt cacgccatcg gtggcattca ccagcgtccc
     7501 atcgccgtta aacagtgagg cattaagggc gacagaagta tcctgcccgg tcaggcgcaa
     7561 aaccgtacca tcgctgatgt cgatagtgcc gctggaagta gcatcaatag attgaatatc
     7621 ctgatccgcg ccagtaacga acgtcgcccc atcaccgacc cacagtgacg aagcataagg
     7681 aaggatatcc gcgacatcgc ctttcagggt gccttcttcg acacgtaccg ccgactgcgt
     7741 tgtaccgctg gcggtcagct ccagcgtacc cgcccccgtt ttagtcaatg tgctaccctc
     7801 atcctgatgc tgcccgctgc tgtcagccat cagtgcgccc cactgcgtgt ctacccccgc
     7861 atcaactgcc acttcaccgt cggcgcgcat ttcaatatca cgcccatgct cagtcgtcgt
     7921 agagcgagta tcgacattgg cagtaatctc catcacgctt tcttgttgct tatcggtaaa
     7981 gataacctgg cggttatagt gggtatctcc aagctgtgag ttgtcgctca cctccagcat
     8041 accggaggcg atttgtgttg tgcccaaata actattattg tttgccagac ttacctgccc
     8101 gtcgcccgca tcgaccacca cgtagacgcc atctttcccg tcaccgccga tatgcatatc
     8161 gtttccacct gcaagatcta ccacatcctg actgccgata accgttccgc tgctgccgct
     8221 gacttcaagg ccatcgttgt atgatgtgcc actctgccag gtggagaaat cagagagatc
     8281 gagcacaccg ccgtttaaca cgatcgactg cggatcgtcc tggagagcgg taagatctgc
     8341 cgcgtcgcct tccagcgaaa gcaccgcacc atcatcaacg actatatcgc cggttagcgc
     8401 catcgactgc gcccccgaca gcacgtagct gccgttttgc gcaatggtga gctgaccagc
     8461 accttcgatg gtgccagcaa aactgccttg attaacagta acattgccac cagcatcgat
     8521 atttaaagtg ccattctgaa agcccgtcaa tgagtgcaca aaggtttgtt gggtggagcc
     8581 aacgtttagt tctgcctgat tctggtactt atcaatactc cctatcgtca gaccgtagca
     8641 gtcttgcgga tcgtcctggc aatgcgtatc gccgacattc atcagggagt tgctgcgacc
     8701 cagggtaact tcaccgtttt caatctgcat ctcaccagta aagtcattgt tatctgcatt
     8761 aagtaccaga tcgccggaac ctgttttggt gatcaacccg gtaccagcaa tagagtcaac
     8821 agctccgtca ttctctgtat tgccaataac cagcgttttt ccgtcggcaa tatcaaaggt
     8881 aacttcactt aagccgagat acataaagcc acccgccgca gtggaaggac catctccata
     8941 gcctgctgcg ctgttgttct catccactaa cacgccgccg ttctggctgt agctgtcatc
     9001 aacagaaata tcaataagat agggagccgt cgcgctattg gtatatatcg ccccgccata
     9061 atcttcggca gtgttatttg taaaggcggt gttatttatt atcgtataac ctgaaggatg
     9121 cttgctgtcg ctattattat cggtaacatc gattgccccg ccatcgccat cactgtaact
//
LOCUS       DAZKHF020000180         9137 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2201S31241
            SAMN43267800-rid25106413.denovo.181, whole genome shotgun sequence.
ACCESSION   DAZKHF020000180 DAZKHF020000000
VERSION     DAZKHF020000180.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267800
            Sequence Read Archive: SRR30307263
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 9137)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9137)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 9137)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350581.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350581.2
            Genome Coverage       :: 151x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:21:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,652
            CDSs (total)                      :: 5,557
            Genes (coding)                    :: 5,320
            CDSs (with protein)               :: 5,320
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 237
            CDSs (without protein)            :: 237
            Pseudo Genes (ambiguous residues) :: 0 of 237
            Pseudo Genes (frameshifted)       :: 102 of 237
            Pseudo Genes (incomplete)         :: 130 of 237
            Pseudo Genes (internal stop)      :: 52 of 237
            Pseudo Genes (multiple problems)  :: 41 of 237
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9137
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267800-rid25106413.denovo.181"
                     /serotype="O 26:H11"
                     /isolate="2201S31241"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-01"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            305..457
                     /gene="hokE"
                     /locus_tag="ACHOSC_RS17265"
                     /old_locus_tag="pgaptmp_003453"
     CDS             305..457
                     /gene="hokE"
                     /locus_tag="ACHOSC_RS17265"
                     /old_locus_tag="pgaptmp_003453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308648.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin HokE"
                     /protein_id="HGU4549337.1"
                     /translation="MLTKYALVAVIVLCLTVLGFTLLVGDSLCEFTVKERNIEFKAVL
                     AYEPKK"
     gene            complement(579..1199)
                     /gene="entD"
                     /locus_tag="ACHOSC_RS17270"
                     /old_locus_tag="pgaptmp_003454"
     CDS             complement(579..1199)
                     /gene="entD"
                     /locus_tag="ACHOSC_RS17270"
                     /old_locus_tag="pgaptmp_003454"
                     /EC_number="6.3.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415115.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009366 - enterobactin synthetase complex
                     [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008897 - holo-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009239 - enterobactin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enterobactin synthase subunit EntD"
                     /protein_id="HGU4549338.1"
                     /translation="MKTTHTSLPFAGHTLHFVEFDPANFCEQDLLWLPHYAQLQHAGR
                     KRKAEHLAGRIAAIYALREYGYKCVPAIGELRQPVWPAEVYGSISHCGATALAVVSRQ
                     PIGVDIEEIFSAQTATELTDNIITPAEHERLADCGLAFSLALTLAFSAKESAFKASEI
                     QTDAGFLDYQIISWNKQQVIIHRENEMFAVHWQIKEKIVITLCQHD"
     gene            complement(1236..1334)
                     /locus_tag="ACHOSC_RS17275"
                     /old_locus_tag="pgaptmp_003455"
                     /pseudo
     CDS             complement(1236..1334)
                     /locus_tag="ACHOSC_RS17275"
                     /old_locus_tag="pgaptmp_003455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518748.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(1374..3614)
                     /gene="fepA"
                     /locus_tag="ACHOSC_RS17280"
                     /old_locus_tag="pgaptmp_003456"
     CDS             complement(1374..3614)
                     /gene="fepA"
                     /locus_tag="ACHOSC_RS17280"
                     /old_locus_tag="pgaptmp_003456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="siderophore enterobactin receptor FepA"
                     /protein_id="HGU4549339.1"
                     /translation="MNKKIHSLALLVNLGIYGVAQAQEPTDTPVSHDDTIVVTAAEQN
                     LQAPGVSTITADEIRKNPVARDVSEIIRTMPGVNLTGNSTSGQRGNNRQIDIRGMGPE
                     NTLILIDGKPVSSRNSVRQGWRGERDTRGDTSWVPPEMIERIEVLRGPAAARYGNGAA
                     GGVVNIITKKGSGEWHGSWDAYFNAPEHKEEGATKRTNFSLTGPLGDEFSFRLYGNLD
                     KTQADAWDINQGHQSARAGTYATTLPAGREGVINKDINGVVRWDFAPLQSLELEAGYS
                     RQGNLYAGDTQNTNSDAYTRSKYGDETNRLYRQNYALTWNGGWDNGVTTSNWVQYEHT
                     RNSRIPEGLAGGTEGKFNEKATQDFVDIDLDDVMLHSEVNLPIDILVNQTLTLGTEWN
                     QQRMKDLSSNTQALTGANTGGAIDGMSATDRSPYSKAEIFSLFAENNMELTDSTIVTP
                     GLRFDHHSIVGNNWSPALNISQGLGDDFTLKMGIARAYKAPSLYQTNPNYILYSKGQG
                     CYASAGGCYLQGNDDLKAETSINKEIGLEFKRDGWLAGVTWFRNDYRNKIEAGYVAVG
                     QNAVGTDLYQWDNVPKAVVEGLEGSLNVPVSETVMWTNNITYMLKSENKTTGDRLSII
                     PEYTLNSTLSWQAREDLSMQTTFTWYGKQQPKKYNYKGQPAVGPETKEISPYSIVGLS
                     ATWDVTKNVSLTGGVDNLFDKRLWRAGNAQTTGDLAGANYIAGAGAYTYNEPGRTWYM
                     SVNTHF"
     gene            3857..5058
                     /gene="fes"
                     /locus_tag="ACHOSC_RS17285"
                     /old_locus_tag="pgaptmp_003457"
                     /pseudo
     CDS             3857..5058
                     /gene="fes"
                     /locus_tag="ACHOSC_RS17285"
                     /old_locus_tag="pgaptmp_003457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308651.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0008849 - enterochelin esterase activity
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="enterochelin esterase"
     gene            5061..5279
                     /gene="ybdZ"
                     /locus_tag="ACHOSC_RS17290"
                     /old_locus_tag="pgaptmp_003458"
     CDS             5061..5279
                     /gene="ybdZ"
                     /locus_tag="ACHOSC_RS17290"
                     /old_locus_tag="pgaptmp_003458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enterobactin biosynthesis protein YbdZ"
                     /protein_id="HGU4549340.1"
                     /translation="MAFSNPFDDPQGAFYILRNAQGQFSLWPQQCALPAGWDIVCQPQ
                     SQASCQQWLEVHWRTLTPANFTQLQEAQ"
     gene            5276..>9137
                     /gene="entF"
                     /locus_tag="ACHOSC_RS17295"
                     /old_locus_tag="pgaptmp_003459"
     CDS             5276..>9137
                     /gene="entF"
                     /locus_tag="ACHOSC_RS17295"
                     /old_locus_tag="pgaptmp_003459"
                     /EC_number="6.3.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0031177 - phosphopantetheine binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enterobactin non-ribosomal peptide synthetase
                     EntF"
                     /protein_id="HGU4549341.1"
                     /translation="MSQHLPLVAAQPGIWVAEKLSDLPSAWSVAHYVELTGDVDAPLL
                     ARAVVVGLAQADTLRMRFTEDNGEVWQWVDDALIFELPEIIDLRTNIDPHGTAQALML
                     ADLQQDLRVDSGKPLVFHQLIHVADNRWYWYQRYHHLLVDGFSFPAITRQIANIYCTW
                     LRGEPTPASPFTPFADVVEEYQQYRESEAWQRDAAFWAEQRRQLPPPASLSPAPLAGR
                     SASADILRLKLEFTDGEFRQLATQLSGVQRTDLALALAALWLGRLCNRMDYAAGFIFM
                     RRLGSAALTASGPVLNVLPLGIHIAAQETLPELATRLAAQLKKMRRHQRYDAEQIVRD
                     SGRAAGEEPLFGPVLNIKVFDYQLDIPGVQTQTHTLATGPVNDLELALFPDEHGDLSI
                     EILANKQRYDEPTLIQHAERLKMLIAQFAADPALLCGDVDIMLPGEYAQLAQINATQV
                     EIPETTLSALVAEQAAKTPDAPALADARYLFSYREMREQVVALANLLRERGVKPGDSV
                     AVALPRSVFLTLALHAIVEAGAAWLPLDTGYPDDRLKMMLEDARPSLLITTDDQLPRF
                     SDVPNLTSLCYNAPLTPQGSAPLQLSQPHHTAYIIFTSGSTGRPKGVMVGQTAIVNRL
                     LWMQNHYPLTGEDVVAQKTPCSFDVSVWEFFWPFIAGAKLVMAEPEAHRDPLAMQQFF
                     AEYGVTTTHFVPSMLAAFVASLTPQTARQNCATLKQVFCSGEALPADLCREWQQLTGA
                     PLHNLYGPTEAAVDVSWYPAFGEELAQVRGSSVPIGYPVWNTGLRILDAMMHPVPPGV
                     AGDLYLTGIQLAQGYLGRPDLTASRFIADPFAPGERMYRTGDVARWLDNGAVEYLGRS
                     DDQLKIRGQRIELGEIDRVMQALPDVEQAVTHACVINQAAATGGDARQLVGYLVSQSG
                     LPLDTSALQAQLRETLPPHMVPVVLLQLPQLPLSANGKLDRKALPLPELKTQASGRAP
                     KAGSETIIAAAFASLLGCDVQDADADFFALGGHSLLAMKLAAQLSRQFARQVTPGQVM
                     VASTVAKLATIIDGEEDSSRRMGFETILPLREGNGPTLFCFHPASGFAWQFSVLSRYL
                     DPQWSIIGIQSPRPHGPMQTATNLDEVCEAHLATLLEQQPHGPYYLLGYSLGGTLAQG
                     IAARLRARGEQVAFLGLLDTWPPETQNWQEKEANGLDPEVLAEINREREAFLAAQQGS
                     TSTELFTTIEGNYADAVRLLTTAHSVPFDGKATLFVAERTLQEGMSPERAWSPWIAEL
                     DIYRQDCAHVDIISPGAFVKIGPII"
BASE COUNT         1970 a         2427 c         2536 g         2204 t
ORIGIN      
        1 atggtaaggc acctgcttta cactttcgcc cgcggtcagt gatggctgcg ggcgatttcg
       61 ttttaagagc aatgatgagt tttgccgaca gaagtccgaa acaggattca ctgtataaat
      121 aaacagatat tttgttgagg aagggtaaga taacggcggg tgcctgaagc tttccggttt
      181 caggttaact ctgaggtctg gaaagatgaa gccccaggag atatttctat caatcctggg
      241 gctgccactc cgagcctcaa caactcggat ggtagtccct tcttcgcatg gaggcaatat
      301 aaacatgctg acgaaatatg cccttgtggc agtaatagtg ctgtgtttga cggtactggg
      361 atttacgctt ctggtcggag actcgctgtg tgagttcacc gtaaaggaac gtaatattga
      421 gtttaaggct gttctcgctt acgaaccgaa gaagtagccg ttgtgcgggg agcaatcccc
      481 gcatatccgg ttgtcaggtc agagtggtaa ggcacctgct ttacactttc gcccgcggtc
      541 agtgatggct gcgggcgaat cgtaccagat gttgtcaatt aatcgtgttg gcacagagtt
      601 atgactattt tttcttttat ctgccagtgc acagcaaaca tctcattctc acgatgaatg
      661 atgacctgct gtttattcca gctaattatc tgatagtcca gaaaacctgc atcagtttgg
      721 atctcacttg ccttaaatgc gctctctttg gcggaaaatg ccagtgtcag cgccagagaa
      781 aaggctaaac cgcagtctgc gagtcgctcg tgttctgctg gtgtaataat gttgtctgtc
      841 aattcggtcg ctgtttgcgc agaaaaaatt tcttcgatat caacgccaat cggttgacgg
      901 gaaaccacgg ccaatgccgt agccccgcag tggctaatac tgccgtatac ctccgcaggc
      961 cacaccggtt ggcgtagctc gccgattgcg ggcacacatt tatagccata ttcccgcagc
     1021 gcataaattg cagcgatccg cccggctaaa tgttcggctt tgcgcttccg tccagcgtgt
     1081 tgcagttgcg cgtaatgcgg cagccagagt aaatcctgct cacaaaaatt cgccggatcg
     1141 aactcaacaa aatgcagcgt atgtccggca aaggggaggg aggtatgcgt agttttcatg
     1201 tcgaccatat tagagactga tgacaaacgc taaactgcct gatgcgctac gcttatcagg
     1261 cctacatggt cctgcaatat attgaattgg caagattttt gtaggccgga taaggcgttc
     1321 acgccgcatc cggcatgaac gacgcgcact ttgtcaacaa tctgacgtta gcatcagaag
     1381 tgagtgttta cgctcatgta ccacgtacgt cccggctcgt tataggtata cgccccggca
     1441 cccgcgatat agttggcccc tgccagatcg cccgtggtct gggcattacc cgcacgccac
     1501 aaacgtttgt cgaacaggtt gtccacgccg ccggtcagac tgacattctt cgtcacatcc
     1561 caggtcgcgc tcagacccac aatgctgtaa ggactgattt ctttggtttc cgggccaacc
     1621 gctggctgac ctttgtagtt gtattttttc ggctgctgct taccgtacca ggtgaaggtc
     1681 gtttgcatcg acaaatcttc ccgcgcctgc cagctcagcg ttgagttcaa cgtgtactcc
     1741 gggatgatcg acaaacggtc gcccgtggtt ttgttttcac tcttcagcat ataagtgatg
     1801 ttattggtcc acatcaccgt ttcgctaact ggtacgttta acgatccttc cagaccttca
     1861 accactgctt tcggcacgtt atcccactga tagagatcgg tgccgactgc gttttgccct
     1921 acagccacat agcccgcttc aatcttattg cgataatcgt tacggaacca ggtgacgccc
     1981 gccagccagc cgtcgcgttt gaactccaga ccaatctctt tgttgatgct ggtttctgct
     2041 ttcaggtcat cgttaccttg cagatagcag ccgcccgcgc tggcatagca gccctgacct
     2101 ttactgtaga gaatgtagtt cgggttagtc tggtacaggc tcggtgcttt ataagcacgg
     2161 gcaatgccca ttttgagcgt gaagtcatcg cctaaacctt gcgatatgtt cagcgccggg
     2221 ctccagttat tgccgacaat actgtgatga tcgaaacgca gccccggcgt tacgatggtg
     2281 ctgtcagtca gctccatgtt gttttcggca aacagcgaga aaatttctgc ttttgaatac
     2341 gggctacggt cggtggcact cataccatca atagcgccgc cggtgttcgc cccggtcagc
     2401 gcctgggtat tggaactcaa gtccttcatc cgttgctgat tccactccgt acccagcgtc
     2461 agcgtctggt taacgaggat atcaatcggc aggttaactt cgctgtgcag catcacgtca
     2521 tcgagatcga tatcgacgaa atcctgtgtc gctttttcgt taaatttccc ttcggtaccg
     2581 cccgccagac cttccggaat acgcgagtta cgggtgtgtt catactgcac ccagttgctg
     2641 gtggtcacgc cgttatccca gccaccgttc caggtcagcg cgtagttctg gcgatacagg
     2701 cggttggttt catcgccata tttcgagcga gtgtaagcgt cagagttggt gttctgggta
     2761 tcgcccgcat acaggttacc ctggcggctg taacccgctt ccagttccag cgattgcaat
     2821 ggtgcgaagt cccagcgcac cacgccgtta atatctttat tgatcacccc ttcgcgcccg
     2881 gctggtaacg tcgtggcata cgttccggca cgcgcggact gatggccctg gttgatatcc
     2941 cacgcgtcag cctgggtttt gtcgaggttg ccatacaagc ggaagctgaa ttcgtcgccc
     3001 agcggaccgg tcaggctgaa gttggtgcgt ttagtggctc cttcctcttt atgttctggt
     3061 gcattgaaat aagcgtccca ggagccgtgc cactcaccgc tgcctttttt ggtaatgatg
     3121 ttaaccacgc cgcccgccgc gccgttgcca taacgcgcag ctgccggacc acgcagaact
     3181 tcaatacgtt caatcatttc aggtggcacc caggaggtat caccacgggt atcgcgctcg
     3241 ccacgccagc cctgacgcac cgagttacgg ctgcttaccg gcttgccgtc aatcaaaatc
     3301 agcgtgtttt ccggacccat accgcgaata tcaatctgtc ggttattccc acgctgacca
     3361 ctggtggagt taccggtcag gttaacgcct ggcatggtac ggatgatctc cgacacatcg
     3421 cgggcaacgg ggtttttgcg gatttcatct gcggtgatgg tcgaaacgcc aggcgcctgt
     3481 aagttctgct cggcggcggt aacgacaatg gtatcgtcat gtgaaacagg agtatcggtc
     3541 ggctcttgtg cctgcgctac cccataaatc cccagattga ccaacaaggc cagggaatga
     3601 atcttcttgt tcattgtttt attcctgcat ttttgccacg aattgcaact gtcgggcatg
     3661 gtcgtcctca acacgacgca tcccgctacc gcgaaaacct ttgatcctga aagacacgca
     3721 gtgcagttgg ttaataaatg tccgcgcttc ccacggcgcg ccattacgct attgcaaatg
     3781 caaatagtta tcaataatat tatcaatata tttctgcaat caatgaaaaa ttgcacagta
     3841 aacatggggt tatggtgtga cggcgttaaa agtaggaagt gagagctggt ggcagtcgaa
     3901 acatggcccg gaatggcagc gtctgaatga cgaaatgttt gaggtcactt tctggtggcg
     3961 tgatccccaa ggttctgaag aatactcgac gataaagcgc gtatggatct acatcactgg
     4021 tgtgaccgat catcatcaga acagccagcc ccagtcgatg cagcgaattg caggtactga
     4081 tgtctggcag tggacgacac aactcaatgc caactggcgc ggcagctact gctttattcc
     4141 caccgaacgc gatgacattt tttctgcacc atcccccgat cgcctcgaat tacgtgaagg
     4201 ctggcgaaaa ctattacccc aggcaatagc cgatccgctg aatccacaaa gctggaaagg
     4261 cgggcgaggg cacgctgttt ctgcactcga aatgccgcaa gcgcctctgc aaccgggatg
     4321 ggattgtccg caagcgccag aaatagcagc caaagaaatt atctggaaaa gtgaacggtt
     4381 gaaaaattca cgtcgtgtat ggatttttac caccggcgat gcaacagcag aagaacgtcc
     4441 gctggcagtt ttgctcgatg gcgaattttg ggcgcaaagt atgcccgtct ggcctgtgct
     4501 gacttcgctg acccatcgtc agcaacttcc tcccgccgtg tatgtgttga tcgacgctat
     4561 cgacaccacg caccgcgccc acgaactgcc gtgtaatgcg gatttctggc tggcagtaca
     4621 gcaagagtta ttacccctgg tgaaagctat tgcccctttt agcgatcgcg ccgatcgcac
     4681 cgtggtcgcc gggcagagtt ttggtgggct gtccgcgctg tatgccggac tgcactggcc
     4741 tgaacgcttt ggctgtgtat taagccagtc aggatcgtac tggtggccgc atcggggcgg
     4801 gcagcaagag ggcgtgttac ttgaaaagct taaagctggt gaagttagcg ccgaaggtct
     4861 gcgcattgtg ctggaagcgg gtattcgcga gccgatgatt atgcgggcca atcaggcgct
     4921 gtatgcgcaa ttacacccca taaaagaatc cattttctgg cgtcaggttg acggcggaca
     4981 tgatgcgctt tgttggcgcg gtggcttgat gcaggggcta atcgacctct ggcaaccact
     5041 ttccatgaca ggagttgaat atggcattca gtaatccctt cgatgatcca cagggagcgt
     5101 tttacatttt gcgcaatgcg caggggcaat tcagtctgtg gccgcaacaa tgcgccttac
     5161 cggcaggctg ggacattgtg tgtcagccgc agtcacaggc gtcctgccag cagtggctgg
     5221 aagtccactg gcgtactctg acaccggcca atttcaccca gttgcaggag gcacaatgag
     5281 ccagcattta cctttggtcg ccgcacagcc cggcatctgg gtggcagaaa aactgtcgga
     5341 tttaccctcc gcctggagcg tggcgcatta cgttgagtta actggagatg ttgatgcgcc
     5401 attactggcc cgcgcggtgg ttgtcggact agcgcaagca gatacgttgc ggatgcgttt
     5461 tacggaagat aacggcgaag tctggcagtg ggtcgatgat gcgctgatat tcgaactgcc
     5521 agaaattatc gacctgcgaa ccaatattga tccgcacggt actgcgcagg cattaatgct
     5581 ggcggatttg caacaagatt tgcgcgtcga tagcggtaaa ccactggtct ttcatcagct
     5641 gatacatgtt gcggataacc gctggtactg gtatcagcgt tatcaccatt tgctggtcga
     5701 tggcttcagt ttcccggcca ttactcgcca gatcgccaat atttactgca catggctgcg
     5761 tggcgaacca acgcctgctt cgccgtttac gcctttcgct gatgtagtgg aagagtacca
     5821 gcaataccgc gaaagcgaag cctggcagcg tgatgcggca ttctgggcag aacagcgtcg
     5881 tcaactgccg ccgcccgcgt cactttctcc ggcaccttta gcggggcgca gcgcttcggc
     5941 agatattctg cgcctgaaac tggaatttac cgacggggaa ttccgccagc tggctacgca
     6001 actttcaggt gtgcagcgta ccgatttagc ccttgcgctg gcagccttgt ggctggggcg
     6061 attgtgcaat cgcatggact atgccgccgg atttatcttt atgcgtcgac tgggctcggc
     6121 ggcgctgacg gctagcggac ccgtgctcaa cgttttaccg ttgggtattc acattgcggc
     6181 gcaagaaacg ctgccggaac tggcaacccg actggcagca caactgaaaa aaatgcgtcg
     6241 tcatcaacgt tacgatgccg aacaaattgt ccgtgacagc gggcgagcgg caggagagga
     6301 accgctgttc ggtccggtac tcaatatcaa ggtatttgat taccaactgg atattcctgg
     6361 tgttcagacg caaacccata ccctggcaac cggtccggtt aatgaccttg aactggccct
     6421 gttcccggat gaacacggtg atttgagtat tgagatcctc gccaataaac agcgttacga
     6481 tgagccaacg ttaatccagc atgctgaacg cctgaaaatg ctgattgccc agttcgccgc
     6541 ggatccggcg ctgttgtgcg gcgatgtcga tattatgctg ccaggtgagt atgcgcagct
     6601 ggcgcagatc aacgccactc aggttgagat tccagaaacc acgctgagcg cgctggtggc
     6661 agaacaagcg gcaaaaacac cggatgctcc ggcgctggca gatgcgcgtt acctgttcag
     6721 ctatcgggaa atgcgcgagc aggtggtggc gctggcgaat ctgctgcgtg agcgcggcgt
     6781 taaaccaggg gacagcgtgg cggtggcact accgcgctcg gtctttttga ccctggcact
     6841 ccatgcaata gttgaagctg gcgcggcctg gctaccgctg gataccggct atccggacga
     6901 tcgcctgaaa atgatgctgg aagatgcgcg tccgtcgctg ttaattacca ccgacgatca
     6961 actgccgcgc tttagcgatg ttcccaattt aacaagcctt tgctataacg ccccgcttac
     7021 cccccagggc agtgcgccgc tgcaactttc acaaccgcac cacacggctt atatcatctt
     7081 tacctctggc tccaccggca ggccgaaagg ggtaatggtc gggcagacgg ctatcgttaa
     7141 ccgcctgtta tggatgcaaa atcactaccc gcttacaggc gaagatgtcg ttgcccaaaa
     7201 aacgccgtgc agttttgatg tctcggtgtg ggagtttttc tggccgttta tcgcaggggc
     7261 aaaactggtg atggctgaac cggaagcgca ccgtgacccg ctcgctatgc agcaattctt
     7321 tgccgaatat ggcgtaacga ccacgcactt tgtgccgtcg atgctggcgg catttgttgc
     7381 atcgctgacg ccgcaaaccg ctcgccagaa ttgcgcgacg ttgaaacagg ttttctgtag
     7441 tggtgaggcc ttaccggctg atttatgccg cgaatggcaa cagttaaccg gcgcgccgtt
     7501 gcataacctg tatggcccga cggaagcggc ggtagatgtc agctggtatc cggcttttgg
     7561 cgaggaactg gcacaggtgc gcggcagcag tgtgccgatt ggttatccgg tatggaatac
     7621 gggtctgcgt attcttgatg cgatgatgca tccggtgccg ccgggtgtgg cgggtgatct
     7681 ctatctcacc ggcatccaac tggcgcaggg gtatcttgga cgacccgatc tgaccgccag
     7741 ccgttttatt gccgatcctt ttgccccagg tgaacggatg tatcgtaccg gagacgtcgc
     7801 ccgctggctg gataacggcg cggtggagta cctcgggcgc agtgacgatc agctaaaaat
     7861 tcgcgggcag cgtatcgaac tgggcgaaat cgatcgcgtg atgcaggcgc tgccggatgt
     7921 cgaacaggca gttacccacg cctgtgtgat taaccaggcg gcagccaccg gtggtgatgc
     7981 gcgtcagttg gtgggctatc tggtgtcgca atcgggactg ccgttggata ccagcgcatt
     8041 acaggcacag cttcgcgaaa cattgccgcc gcatatggtg ccggtcgttc tgctgcaact
     8101 tccgcagttg ccgcttagcg ccaacggcaa gctggatcgc aaagccttac cgttgcctga
     8161 actgaagaca caagcgtcgg gacgtgcacc gaaagcgggc agtgaaacga ttatcgccgc
     8221 ggcattcgcg tcgttgctgg gttgtgacgt gcaggatgcc gatgctgatt tctttgcgct
     8281 gggcggtcat tcgctactgg caatgaaact agcagcgcag ttaagtcgac agtttgctcg
     8341 tcaggtgacg ccggggcagg tgatggtcgc gtcaaccgtc gccaaactgg caacgattat
     8401 tgatggtgaa gaggacagct cccggcgcat gggattcgaa accattctgc cgttgcgtga
     8461 aggtaatggc ccgacgctgt tttgtttcca tccggcgtcc ggttttgcct ggcagtttag
     8521 cgtgctctcg cgttatctcg atccacaatg gtcgattatt ggcattcagt cgccgcgccc
     8581 tcatggaccc atgcagacag cgacaaacct ggatgaagtc tgcgaagcgc atctggcaac
     8641 gttacttgag caacaaccgc acggccctta ttacctgctg gggtattccc ttggcggtac
     8701 gctggcgcag ggtattgcgg cgcgactgcg tgcccgtggc gaacaggtgg catttcttgg
     8761 tttgctggat acctggccgc cagagacgca aaactggcag gaaaaagagg ctaatggtct
     8821 ggacccggaa gtgctggcgg agattaaccg cgaacgcgag gccttcctgg cggcacagca
     8881 gggaagtact tcaacggagt tgtttaccac cattgaaggc aactacgctg atgctgtgcg
     8941 cttgttaacc accgcccata gcgttccgtt tgatggaaaa gcaacgttat ttgttgctga
     9001 gcgcacactt caggaaggta tgagtcccga acgtgcctgg tcgccgtgga ttgcggagct
     9061 ggatatctat cgtcaggatt gtgcgcatgt ggatattatc tctccagggg cgtttgtaaa
     9121 aattgggccg attattc
//
LOCUS       DAZKHF020000181         9019 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2201S31241
            SAMN43267800-rid25106413.denovo.182, whole genome shotgun sequence.
ACCESSION   DAZKHF020000181 DAZKHF020000000
VERSION     DAZKHF020000181.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267800
            Sequence Read Archive: SRR30307263
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 9019)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9019)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 9019)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350581.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350581.2
            Genome Coverage       :: 151x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:21:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,652
            CDSs (total)                      :: 5,557
            Genes (coding)                    :: 5,320
            CDSs (with protein)               :: 5,320
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 237
            CDSs (without protein)            :: 237
            Pseudo Genes (ambiguous residues) :: 0 of 237
            Pseudo Genes (frameshifted)       :: 102 of 237
            Pseudo Genes (incomplete)         :: 130 of 237
            Pseudo Genes (internal stop)      :: 52 of 237
            Pseudo Genes (multiple problems)  :: 41 of 237
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9019
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267800-rid25106413.denovo.182"
                     /serotype="O 26:H11"
                     /isolate="2201S31241"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-01"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            <1..1140
                     /locus_tag="ACHOSC_RS17300"
                     /old_locus_tag="pgaptmp_003460"
     CDS             <1..1140
                     /locus_tag="ACHOSC_RS17300"
                     /old_locus_tag="pgaptmp_003460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HGU4549342.1"
                     /translation="FTNKQILPTLILSLAFLSGLIYLVETIVIIHKPINGSTLIQTKS
                     NDVSIFYIFRQLSFICLTSLALFCYGKDNILDNNKKKTGILLLALIPFLVFPLLAHNL
                     SSYNADYSLYVVDYCPDNHTATWGINYTKILVCLWAFLLFFIIMRTRLASELWPLIAL
                     LCLASLCCNLLLLTLDEYNYTIWYISRGIEVSSKLFVVSFLIYNIFQELQLSSKLAVH
                     DVLTNIYNRRYFFNSVESLLSRPVVKDFCVMLVDINQFKRINAQWGHRVGDKVLVSIV
                     DIIQQSIRPDDILARLEGEVFGLLFTELNSAQAKIIAERMRKNVELLTGFSNRYDVPE
                     QMTISIGTVFSTGDTRNISLVMTEADKALREAKSEGGNKVIIHHI"
     gene            1321..2811
                     /gene="dgcJ"
                     /locus_tag="ACHOSC_RS17305"
                     /old_locus_tag="pgaptmp_003461"
     CDS             1321..2811
                     /gene="dgcJ"
                     /locus_tag="ACHOSC_RS17305"
                     /old_locus_tag="pgaptmp_003461"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416300.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052621 - diguanylate cyclase activity
                     [Evidence IEA];
                     GO_process: GO:0043709 - cell adhesion involved in
                     single-species biofilm formation [Evidence IEA];
                     GO_process: GO:1902201 - negative regulation of
                     bacterial-type flagellum-dependent cell motility [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase DgcJ"
                     /protein_id="HGU4549343.1"
                     /translation="MKLHHRMLRHFIAASVIVLTSSFLIFELVASDRAMSAYLRYIVQ
                     KADSSFLYDKYQNQSIAAHVMRALAAEQSEVSPEQRRAICEAFESANNTHGLNLTAHK
                     YPGLRGTLQTASTDCDTIVEAAALLPAFDQAVEGNRHQDDYGSGLGMAEEKFHYYLDL
                     NDRYVYFYEPVNVEYFAMNNWSFLQSGSIGIDRKDIEKVFTGRTVLSSIYQDQRTKQN
                     VMSLLTPVYVAGQLKGIVLLDINKNNLRNIFYTHDRPLLWRFLNVTLTDTDSGRDIII
                     NQSEDNLFQYVSYVHDLPGGIRVSLSIDILYFITSSWKSVLFWILTALILLNMVRMHF
                     RLYQNVSRENISDAMTGLYNRKILTPELEQRLQKLVQSGSSVMFIAIDMDKLKQINDT
                     LGHQEGDLAITLLAQAIKQSIRKSDYAIRLGGDEFCIILVDSTPQIAAQLPERIEKRL
                     QHIAPQKEIGFSSGIYAMKENDTLHDAYKASDERLYVNKQNKNSRS"
     gene            2854..3357
                     /gene="yeaK"
                     /locus_tag="ACHOSC_RS17310"
                     /old_locus_tag="pgaptmp_003462"
     CDS             2854..3357
                     /gene="yeaK"
                     /locus_tag="ACHOSC_RS17310"
                     /old_locus_tag="pgaptmp_003462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mischarged aminoacyl-tRNA deacylase"
                     /protein_id="HGU4549344.1"
                     /translation="MTEMAKGSVTHQRLIALLSQEGANFRVVTHEAVGKCEAVSEIRG
                     TALGQGAKALVCKVKGNGVNQHVLAILAADQQADLSQLASHIGGLRASLASPAEVDEL
                     TGCVFGAIPPFSFHPKLKLVADPLLFERFDEIAFNAGMLDKSVILKTTDYLRIAQPEL
                     LNFRRTA"
     gene            complement(3358..3462)
                     /gene="yoaI"
                     /locus_tag="ACHOSC_RS17315"
                     /old_locus_tag="pgaptmp_003463"
     CDS             complement(3358..3462)
                     /gene="yoaI"
                     /locus_tag="ACHOSC_RS17315"
                     /old_locus_tag="pgaptmp_003463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416302.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="small membrane protein YoaI"
                     /protein_id="HGU4549345.1"
                     /translation="MNDQMFVETLIITSSFFAIAVVLVLSVLLIERTG"
     gene            3632..4078
                     /gene="yeaL"
                     /locus_tag="ACHOSC_RS17320"
                     /old_locus_tag="pgaptmp_003464"
     CDS             3632..4078
                     /gene="yeaL"
                     /locus_tag="ACHOSC_RS17320"
                     /old_locus_tag="pgaptmp_003464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF441 domain-containing protein"
                     /protein_id="HGU4549346.1"
                     /translation="MFDVTLLILLGLAALGFISHNTTVAVSILVLIIVRVTPLSTFFP
                     WIEKQGLSIGIIILTIGVMAPIASGTLPPSTLIHSFLNWKSLVAIAVGVIVSWLGGRG
                     VTLMGSQPQLVAGLLVGTVLGVALFRGVPVGPLIAAGLVSLIVGKQ"
     gene            complement(4035..4856)
                     /gene="nimR"
                     /locus_tag="ACHOSC_RS17325"
                     /old_locus_tag="pgaptmp_003465"
     CDS             complement(4035..4856)
                     /gene="nimR"
                     /locus_tag="ACHOSC_RS17325"
                     /old_locus_tag="pgaptmp_003465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator NimR"
                     /protein_id="HGU4549347.1"
                     /translation="MMHRLNLNGYEPDRHHEAAVAFCIHAGTDELTSPVHQHRKGQLI
                     LALHGAITCTVENALWMVPPQYAVWIPGGVEHSNQVTANAELCFLFIEPSAVTMPTTC
                     CTLKISPLCRELILTLANRTTTQRAEPMTRRLIQVLFDELPQQPQQQLHLPVSSHPKI
                     RTMVEMMAKGPVEWGSLGQWAGFFAMSERNLARLIVKETGLSFRQWRQQLQLIMALQG
                     LVKGDTVQKVAHTLGYDSTTAFITMFKKGLGQTPGRYIAGLTTVSPQSAKPDPRQ"
     gene            4953..6134
                     /gene="nimT"
                     /locus_tag="ACHOSC_RS17330"
                     /old_locus_tag="pgaptmp_003466"
     CDS             4953..6134
                     /gene="nimT"
                     /locus_tag="ACHOSC_RS17330"
                     /old_locus_tag="pgaptmp_003466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-nitroimidazole transporter"
                     /protein_id="HGU4549348.1"
                     /translation="MTCSTSLSGKNRIVLIAGILMIATTLRVTFTGAAPLLDTIRSAY
                     SLTTAQTGLLTTLPLLAFALISPLAAPVARRFGMERSLFAALLLICAGIAIRSLPSPY
                     LLFGGTAVIGGGIALGNVLLPGLIKRDFPHSVARLTGAYSLTMGAAAALGSAMVVPLA
                     LNGFGWQGALFMLMCFPLLALFLWLPQWRSQQHANLSTSRALHTRGIWRSPLAWQVTL
                     FLGINSLVYYVIIGWLPAILISRGYSEAQAGSLHGLLQLATAAPGLLIPLFLHHVKDQ
                     RGIAAFVALMCAVGAVGLCFMPAHAITWTLLFGFGSGATMILGLTFIGLRASSAHQAA
                     ALSGMAQSVGYLLAACGPPLMGKIHDANGDWSVPLMGVAILSLLMAIFGLCAGRDKEI
                     R"
     gene            6189..6536
                     /gene="yeaO"
                     /locus_tag="ACHOSC_RS17335"
                     /old_locus_tag="pgaptmp_003467"
     CDS             6189..6536
                     /gene="yeaO"
                     /locus_tag="ACHOSC_RS17335"
                     /old_locus_tag="pgaptmp_003467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310528.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF488 domain-containing protein"
                     /protein_id="HGU4549349.1"
                     /translation="MNIQCKRVYDPAEQSDGYRVLVDRLWPRGIKKTDLALDEWDKEI
                     TPSTELRKAFHGEVVDFATFREQYLAELAQHEQEGKRLADIAKKQPLTLLYAAKNTTQ
                     NHALVLADWLRSL"
     gene            complement(6558..6812)
                     /gene="yoaF"
                     /locus_tag="ACHOSC_RS17340"
                     /old_locus_tag="pgaptmp_003468"
     CDS             complement(6558..6812)
                     /gene="yoaF"
                     /locus_tag="ACHOSC_RS17340"
                     /old_locus_tag="pgaptmp_003468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF333 domain-containing lipoprotein YoaF"
                     /protein_id="HGU4549350.1"
                     /translation="MKIISFALPCLLVLAGCSTSSQPEAPKPPQIGMANPASVYCQQK
                     GGTLIPVQTAQGVSNNCKLPGGETIDEWALWRRDHPAGEK"
     gene            6995..8020
                     /gene="dgcP"
                     /locus_tag="ACHOSC_RS17345"
                     /old_locus_tag="pgaptmp_003469"
     CDS             6995..8020
                     /gene="dgcP"
                     /locus_tag="ACHOSC_RS17345"
                     /old_locus_tag="pgaptmp_003469"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416308.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase DgcP"
                     /protein_id="HGU4549351.1"
                     /translation="MSDQIIARVSQSLAKEQSLESLVRQLLEMLEMVTDMESTYLTKV
                     DVEARLQHIMFARNSQKMHIPENFTVSWDYSLCKRAIDENCFFSDEVPDRWGDCIAAR
                     NLGITTFLSTPIHLPDGSFYGTLCAASSEKRQWSERAEQVLQLFAGLIAQYIQKEALV
                     EQLREANAALIAQSYTDSLTGLPNRRAIFENLTTLFSLARHLNHKIMIAFIDLDNFKL
                     INDRFGHNSGDLFLIQVGERLNTLQQNGEVIGRLGGDEFLVVSLNNENADISSLRERI
                     QQQIRGEYHLGDVDLYYPGASLGIVEVDPETTDADSALHAADIAMYQEKKHKQKTPFV
                     AHSAPHS"
     gene            8053..8151
                     /gene="yoaK"
                     /locus_tag="ACHOSC_RS17350"
                     /old_locus_tag="pgaptmp_003470"
     CDS             8053..8151
                     /gene="yoaK"
                     /locus_tag="ACHOSC_RS17350"
                     /old_locus_tag="pgaptmp_003470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YoaK family small membrane protein"
                     /protein_id="HGU4549352.1"
                     /translation="MRIGIIFPVVIFITAVVFLAWFFIGGYAAPGA"
     gene            8154..8228
                     /gene="yoaJ"
                     /locus_tag="ACHOSC_RS17355"
                     /old_locus_tag="pgaptmp_003471"
     CDS             8154..8228
                     /gene="yoaJ"
                     /locus_tag="ACHOSC_RS17355"
                     /old_locus_tag="pgaptmp_003471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YoaJ"
                     /protein_id="HGU4549353.1"
                     /translation="MKKTTIIMMGVAIIVVLGTELGWW"
     gene            complement(8287..8535)
                     /gene="yeaQ"
                     /locus_tag="ACHOSC_RS17360"
                     /old_locus_tag="pgaptmp_003472"
     CDS             complement(8287..8535)
                     /gene="yeaQ"
                     /locus_tag="ACHOSC_RS17360"
                     /old_locus_tag="pgaptmp_003472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HGU4549354.1"
                     /translation="MGILSWIIFGLIAGILAKWIMPGKDGGGFFMTILLGIVGAVVGG
                     WISTLFGFGKVDGFNFGSFVVAVIGAIVVLFIYRKIKS"
     gene            complement(8763..>9019)
                     /locus_tag="ACHOSC_RS17365"
                     /old_locus_tag="pgaptmp_003473"
     CDS             complement(8763..>9019)
                     /locus_tag="ACHOSC_RS17365"
                     /old_locus_tag="pgaptmp_003473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415949.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HGU4549355.1"
                     /translation="GGQVLAVPPAYTSQRCACCGHTAKENRLSQSQFRCQVCGYTANA
                     DVNGARNILAAGHAVLACGEMVQSGRPLKQEPTEMIQATA"
BASE COUNT         2234 a         2182 c         2116 g         2487 t
ORIGIN      
        1 ttcactaaca aacaaatttt accaacactc attttaagcc ttgcattttt aagtggcctt
       61 atctatttag ttgaaaccat tgtaattatc cataaaccaa ttaacggcag tacactgatc
      121 cagacaaagt cgaatgatgt ttctattttc tatattttcc gccaactcag ttttatttgt
      181 ttaacctcgc tggcactctt ttgttatgga aaagacaaca tccttgacaa caataagaaa
      241 aaaacgggaa tcctgttgct ggcgctgatt ccttttttag tttttcccct tctggcacac
      301 aatctgagca gttataacgc tgactattct ttgtatgtcg tcgactactg tccggacaac
      361 catactgcga cctggggaat caactataca aaaatattgg tttgtctgtg ggcattttta
      421 ctgttcttta ttatcatgcg tacacgatta gccagcgaac tatggccgtt aatagcatta
      481 ttatgtctgg catcgctatg ctgcaactta cttctactga ctctggatga gtataattac
      541 accatctggt atatcagtcg cgggattgaa gtttccagta aactgtttgt tgtgtctttt
      601 ctgatttata acatttttca ggaactgcaa ctctccagca aactggcagt tcatgatgtg
      661 ctgaccaata tttataatcg gcgctacttt ttcaacagcg tagagtcatt attgtcgcga
      721 cctgttgtta aggacttctg tgtcatgctg gttgatatta atcagttcaa acgcatcaat
      781 gcccaatggg gacatcgtgt gggtgataaa gtgctggttt caattgtcga tattatccag
      841 caaagcatcc gccctgatga tattttagcg cgactggagg gtgaggtgtt tggcttgcta
      901 tttaccgaac tcaatagtgc ccaggcaaaa atcattgcgg aacgtatgcg taaaaatgtc
      961 gaactcctga ccggctttag taacagatat gatgttcctg aacaaatgac catcagtatt
     1021 ggcacggttt tttcaacggg tgacacgcgt aatatctcgc ttgtcatgac ggaagcagat
     1081 aaagccttac gcgaagcgaa aagcgagggg ggcaacaaag tgattattca tcatatttaa
     1141 gtgcaaaaat attcagagcc atgctttttg cgtggctttt gcatacaatt tattactatt
     1201 acccctaaat ttcccctccc cagcggcgca gagatgagta taattagcgc ccctgtgcca
     1261 ggccgcaatc gaactttatc tggttttctc gtttcactaa ccgaaggagt gccatttatc
     1321 atgaaattgc accatagaat gctccggcat tttatcgccg caagtgtcat tgtgctgaca
     1381 tcttccttcc ttatttttga acttgtcgcc agcgacagag caatgagtgc ctatctgcgc
     1441 tatatcgtgc agaaagcaga ctcctccttt ctttatgata agtatcagaa tcagagtatt
     1501 gccgcgcatg tgatgcgcgc tctcgctgct gagcagtcgg aagtgtcgcc agaacagcgg
     1561 cgcgccatct gcgaggcttt tgagtctgcc aataataccc atggcttaaa cctgactgcc
     1621 cataaatacc cgggcttacg cggcacacta caaaccgcat ccactgactg cgacacaatt
     1681 gtggaagctg cagcactatt acccgctttt gatcaggcag tggaaggcaa ccgccaccag
     1741 gatgattacg gttcaggtct tgggatggcc gaagagaaat ttcactatta tctcgatctc
     1801 aatgaccgct atgtctattt ttatgagccg gttaatgttg aatactttgc gatgaataac
     1861 tggtccttcc tgcagtcagg aagtattggc atcgatcgca aagatattga aaaggtattt
     1921 accgggcgta ccgtattgtc gagcatttac caggatcagc gtactaaaca gaacgtgatg
     1981 agtttgctga cgccggtata tgtcgcaggg cagctaaaag ggattgtgct gctggatatt
     2041 aacaaaaaca atttgcggaa tatcttttac actcatgacc gccctctcct ctggcgtttt
     2101 ctcaatgtca cgctaaccga taccgattcg gggcgcgaca ttatcatcaa ccagagcgaa
     2161 gataatctgt tccagtacgt cagttacgtc catgacttac cgggcggcat tcgtgtctcg
     2221 ttatccattg atattcttta ctttatcacg tcttcgtgga aaagcgttct gttctggatt
     2281 ttgacggcgt taattttgct gaatatggtc cggatgcact tccgtttata ccaaaatgtg
     2341 tcgcgagaaa atattagtga tgcgatgact ggactgtata atcgcaaaat tttaacccct
     2401 gaactggagc agcggttgca gaaactggtg caatccggtt cttcggtgat gtttattgca
     2461 attgacatgg acaagttaaa gcaaataaat gacaccctcg gtcatcagga gggggattta
     2521 gcgattacgt tattagctca ggcgattaaa caatcgattc gtaaaagtga ttatgccatc
     2581 cgactcggtg gcgatgaatt ctgcatcatt cttgtcgatt cgacgccgca aattgcagca
     2641 caactgcctg aacgtatcga aaaacgtctg caacatatcg cgccgcagaa agaaatcggc
     2701 ttctcttccg gtatttacgc gatgaaagaa aacgatacgt tacatgatgc gtataaagct
     2761 tccgatgagc gtttatatgt caataagcag aacaaaaaca gccgttcatg ataaccttct
     2821 gtggttgttt gcttgtaatc tcaggagcgt gaaatgactg aaatggctaa aggaagcgtg
     2881 acgcatcagc ggttaatcgc tttattatca caagaaggtg ctaacttccg cgtcgttacc
     2941 catgaagcgg taggtaaatg tgaagcggtg tcagaaattc gtggcaccgc tctaggccag
     3001 ggtgctaagg cactggtatg taaagtcaaa ggcaatggcg taaatcaaca tgttctggcg
     3061 atcctcgctg ccgatcaaca ggccgatctg agccaactcg ccagccatat aggcggatta
     3121 cgcgcttcgt tggccagccc ggcggaagtc gatgaactca caggctgtgt cttcggcgcg
     3181 atcccccctt tcagcttcca tccaaaactc aaactggttg ccgaccctct actcttcgaa
     3241 cgatttgatg aaatcgcctt caatgcaggg atgctggata aatccgttat tttgaaaacc
     3301 acggattatt tgcgcattgc gcaaccagaa ctgctcaatt tccgccgcac tgcgtaacta
     3361 gcctgtccgt tcaataagca gaacggacaa aaccagcaca acagcaatgg caaaaaacga
     3421 tgacgtgata atcagtgtct cgacgaacat ttgatcgttc atagcatgcc cccgggagat
     3481 agctgttttc acgttattgt tagcgtgcac aaatggcagt ttgatgacag ttcgcctgat
     3541 tttttatgca aaaaacgtaa agattttcta cttccttcct gcagcaagcg taaagtaagc
     3601 aggcttatta ttttttggca aggaaaccac gatgtttgat gtcactttgc tgatcctgct
     3661 cgggttagct gcgctgggct ttatcagtca taacaccact gtcgccgttt caattctggt
     3721 gttgatcatt gtccgcgtca caccgttaag cacctttttc ccgtggattg aaaaacaggg
     3781 gctaagtatc gggataatca ttctgacgat tggcgttatg gcccccatcg ccagcgggac
     3841 gctaccgccc tcaaccttga ttcactcgtt tctgaactgg aaatcgctgg tggcaattgc
     3901 agtaggcgtt attgtctcct ggctgggtgg gcgcggcgtg accttaatgg gcagccagcc
     3961 gcaactggtc gccgggctgc tggtaggcac tgttttaggc gtggcgctgt ttcgcggcgt
     4021 accggtcggc ccgcttattg ccgcgggtct ggtttcgctg attgtgggga aacagtagtt
     4081 aatcccgcaa tatatcggcc tggcgtttgc cccaggcctt tcttaaacat agtaataaag
     4141 gccgttgttg aatcataccc cagcgtatga gcaactttct gtacagtatc gcctttcact
     4201 aatccctgaa gtgccataat cagctgtaac tgctgacgcc actggcgaaa actcaacccc
     4261 gtctctttta ctatcagtcg cgcaagattg cgttcactca tcgcaaaaaa gccagcccat
     4321 tgccctaatg acccccactc gacgggccct ttcgccatca tctccaccat cgtgcggatt
     4381 ttaggatgag aagaaacggg caggtgcagc tgttgttgtg gctgctgagg gagttcatca
     4441 aacaggacct gaattaatct gcgggtcatc ggctcagcac gctgagtcgt cgtcctgttt
     4501 gccagggtta aaatcaattc ccggcataag ggcgatattt tcaatgtgca acatgttgtc
     4561 ggcattgtca ctgcgctggg ttcgataaac agaaaacaga gttcagcgtt agctgtcact
     4621 tgattgctat gttccacccc gcccggtatc caaaccgcat attgcggcgg caccatccac
     4681 aaagcatttt ccaccgtaca ggtaattgca ccatgtaatg ctagtatcag ctgtcctttg
     4741 cggtgctgat gtacaggact ggttaactca tccgttccgg cgtgaataca aaatgcgacc
     4801 gcggcttcat ggtgtcggtc gggttcatag ccattgagat tcaacctgtg catcattttg
     4861 tccgaattta gcgataattt gtcattttag cttgattcaa cataacaata aaaacggtaa
     4921 ggtacagcct cgtttgtaac aatgagaagc atatgacctg ttcaacttca ttaagcggca
     4981 aaaacaggat tgtccttatc gctggcattc tgatgattgc cactacatta cgcgtcacct
     5041 tcaccggcgc agcaccgtta ctcgatacga ttcgttccgc ttactcgctg acgacagcgc
     5101 aaaccggctt attaaccacc ctgccattat tggcctttgc gctaatctca cctttggctg
     5161 ccccggtggc gcgacgtttt ggtatggaac gtagcctgtt tgccgcgtta cttttgatct
     5221 gtgcaggtat cgcaattcgt tctctccctt cgccttactt attatttggc ggtacagcgg
     5281 tcattggcgg tgggattgca ttaggcaatg tcttactgcc aggattaatt aaacgcgatt
     5341 tccctcattc cgtcgccaga cttaccggcg catattccct gacaatggga gctgcagcgg
     5401 cactgggatc ggctatggtc gtgccgctgg ctttgaacgg ttttggctgg caaggcgcgt
     5461 tgttcatgct gatgtgtttt cctctgctgg ctcttttttt atggctgcca cagtggcgaa
     5521 gccaacaaca tgcaaatttg agtacctcgc gcgccttaca tactcggggt atctggcgtt
     5581 cgccgcttgc ctggcaggtc acattgtttc ttgggatcaa ctcactggtc tattacgtga
     5641 ttattggctg gcttccggcg atcctcatca gtcgcggcta tagcgaagca caggcgggtt
     5701 cactgcatgg tttgctgcaa ctagccacag cagctcccgg tttgctgatc cctcttttct
     5761 tacatcatgt gaaagatcag cgtggtattg cagcgttcgt tgccttgatg tgcgcagtgg
     5821 gcgctgttgg gctctgcttt atgccagcgc acgcgatcac ctggactctg cttttcggtt
     5881 ttggttccgg cgcaacaatg atactggggt tgacgttcat tggtcttcgg gcaagttctg
     5941 cgcatcaggc ggcggcactc tcggggatgg cacaatccgt cgggtatttg ctggcagcct
     6001 gtgggccgcc gctgatgggt aaaatacacg atgctaacgg tgactggtcc gtaccactga
     6061 tgggtgttgc catactttca ctactgatgg cgattttcgg actttgcgcc gggagagaca
     6121 aagaaattcg ctaatatccg gtgctatagt gacgtgacaa atcatgcgtg aaagggagaa
     6181 caaacacgat gaatattcag tgcaaacgcg tttatgatcc ggctgaacag agcgatggtt
     6241 atcgcgtact ggtcgaccgc ctctggccgc gcggtatcaa aaaaaccgat ttagcccttg
     6301 atgagtggga taaagaaatc acgccgtcaa cggaactgcg caaagccttt cacggcgaag
     6361 tcgtcgattt tgcaaccttt cgcgagcaat atcttgcaga actggcgcaa cacgagcaag
     6421 aaggaaagcg gctggcggac atcgccaaaa aacagccgct gaccctgctc tacgcagcaa
     6481 aaaacaccac gcagaaccat gcgctggtgc tggccgactg gctacgtagc ttgtgatttt
     6541 agtacagcat ccggcggtta tttttcacca gccggatggt cccgtcgcca caatgcccat
     6601 tcatcaatcg tttcaccgcc cggtaatttg caattgttgc tgaccccttg cgctgtctgc
     6661 actggaatga gcgtcccgcc cttctgctgg caatagaccg acgccggatt tgccatacca
     6721 atctgcggcg gtttaggtgc ttctggctga gaagaggttg aacaaccagc caggaccagc
     6781 aagcaaggca aagcaaaact gataattttc atttattgat ctcacacatt tatctacgct
     6841 tagagtatcg cggtatcgtt ttgttttgca gcactatttt tattacattc actcaaaaca
     6901 tattacgtct tgtttcatct ttgttgatga tgttttatca tgcctgcaaa gattaaataa
     6961 tcagcattta cccgccgtat cctggagttg tttcgtgtca gatcagatta tcgcccgcgt
     7021 ctcgcaatcc cttgccaaag aacagtcact agaaagtctg gtccgacagc ttctggagat
     7081 gctggaaatg gtcactgata tggaatcaac ctacctgacc aaagtggatg tcgaagcgcg
     7141 cctgcagcat attatgtttg cccgtaacag ccagaaaatg cacatcccgg agaattttac
     7201 cgtctcgtgg gattactcgt tatgcaaacg cgctattgat gaaaactgct ttttcagcga
     7261 tgaagtccct gaccgttggg gcgactgtat tgcggcacgc aatcttggaa tcaccacatt
     7321 tctgagtaca ccaattcact taccggatgg atcattctat ggcacgcttt gcgccgccag
     7381 cagtgagaag cgccagtgga gtgaacgcgc ggaacaggtt ttacagttat tcgccggact
     7441 gattgcacaa tatattcaaa aagaggcgtt ggttgaacag ctgcgcgaag ccaatgccgc
     7501 gctgattgcg caatcgtata ccgactcgtt aaccgggcta ccgaatcggc gggcgatttt
     7561 tgaaaatctg acgacgctgt tttctctcgc ccggcatctt aaccataaga taatgatcgc
     7621 gtttatcgat ctggataact tcaaattaat caatgatcgt tttggtcata atagtggcga
     7681 tctgtttctc attcaggttg gcgagcgcct taatacgctc cagcaaaatg gcgaagttat
     7741 tggtcgtctc ggcggtgatg agtttttggt tgtttcacta aataacgaga atgcggatat
     7801 ttcgtcgctg cgagaacgta ttcaacagca aatacgtgga gaatatcact taggtgatgt
     7861 tgatttgtat tatcctggtg ccagtcttgg cattgtagaa gtcgatcctg aaacgaccga
     7921 tgcagacagt gccctgcatg ctgccgatat cgcgatgtat caggagaaaa aacacaaaca
     7981 gaaaacacct tttgtcgcgc attcagcgcc acattcctga ggcgtattca catccttttg
     8041 attggtgata acatgcgaat cggtattatt tttccggttg taatctttat tacagcggtc
     8101 gtatttttag catggttttt tattggcggc tatgctgccc cgggagcata aagatgaaaa
     8161 aaacaacgat tattatgatg ggtgtggcga ttattgtcgt actcggcact gagctgggat
     8221 ggtggtaacg tcacctctaa aaaatagcaa aggctgcctg tgtgcagcct ttgtgcaatt
     8281 taagcgttaa cttttaatct tcctgtagat aaatagcacg acaatcgcac caataacggc
     8341 aaccacgaag ctgccaaaat tgaagccatc gactttacca aagccaaaca gcgtgctgat
     8401 ccatccgcca actacggcac cgactatccc cagcaggata gtcataaaga atccaccacc
     8461 atctttacct ggcatgatcc acttcgccag aataccggca ataagcccaa aaataatcca
     8521 tgacagaatg cccattgttt cctcacttat ctgttttgca ttagcgggtt agtcgctgat
     8581 aaaaagcata gcacaacatc gggagggcaa gatttgtgac gagcatcacg gaggattttt
     8641 ttgcggatgg cacagaaatt gcgccatcaa cgatcagtga taattaccaa ccacaaacat
     8701 catgttcgtt ttccgtgtct tgacatcctc cacgccctga atgacgagga tccctgctac
     8761 gttcaggctg tcgcctgaat catttcggtg ggttcctgct tcaacgggcg gcctgactgc
     8821 accatctctc cacaggcaag aacggcgtgc cccgccgcta aaatgttacg agcgccgttt
     8881 acatcggcgt tcgctgtata tccacatacc tggcatctga attgactttg tgacaggcga
     8941 ttttctttcg ctgtatgacc acagcacgcg caacgctggc ttgtgtacgc tggcggaaca
     9001 gcaagcacct gaccgccac
//
LOCUS       DAZKHF020000182         8927 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2201S31241
            SAMN43267800-rid25106413.denovo.183, whole genome shotgun sequence.
ACCESSION   DAZKHF020000182 DAZKHF020000000
VERSION     DAZKHF020000182.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267800
            Sequence Read Archive: SRR30307263
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 8927)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8927)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 8927)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350581.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350581.2
            Genome Coverage       :: 151x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:21:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,652
            CDSs (total)                      :: 5,557
            Genes (coding)                    :: 5,320
            CDSs (with protein)               :: 5,320
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 237
            CDSs (without protein)            :: 237
            Pseudo Genes (ambiguous residues) :: 0 of 237
            Pseudo Genes (frameshifted)       :: 102 of 237
            Pseudo Genes (incomplete)         :: 130 of 237
            Pseudo Genes (internal stop)      :: 52 of 237
            Pseudo Genes (multiple problems)  :: 41 of 237
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8927
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267800-rid25106413.denovo.183"
                     /serotype="O 26:H11"
                     /isolate="2201S31241"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-01"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            <1..1061
                     /gene="yghO"
                     /locus_tag="ACHOSC_RS17370"
                     /old_locus_tag="pgaptmp_003474"
     CDS             <1..1061
                     /gene="yghO"
                     /locus_tag="ACHOSC_RS17370"
                     /old_locus_tag="pgaptmp_003474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000981816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="protein YghO"
                     /protein_id="HGU4549356.1"
                     /translation="LYPDDPNWIPPLFIERNEHLSAKNPGTDHIIWQAWVAKKAGQIV
                     GRITAQIDTLHRERYGEDTGHFGMIDAIDDPQVFAALFGAAEAWLKSQGASKISGPFS
                     LNINQESGLLIEGFDTPPCAMMPHGKPWYAAHIEQLGYHKGIDLLAWWMQRTDLTFSP
                     ALKKLMDQVRKKVTIRCINRQRFAEEMQILREIFNSGWQHNWGFVPFTEHEFATMGDQ
                     LKYLVPDDMIYIAEIDSAPCAFIVGLPNINEAIADLNGSLFPFGWAKLLWRLKVSGVR
                     TARVPLMGVRDEYQFSRIGPVIALLLIEALRDPFARRKIDALEMSWILETNTGMNNML
                     ERIGAEPYKRYRLYEKQI"
     gene            complement(1068..1832)
                     /gene="glcC"
                     /locus_tag="ACHOSC_RS17375"
                     /old_locus_tag="pgaptmp_003475"
     CDS             complement(1068..1832)
                     /gene="glcC"
                     /locus_tag="ACHOSC_RS17375"
                     /old_locus_tag="pgaptmp_003475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator GlcC"
                     /protein_id="HGU4549357.1"
                     /translation="MKDERRPICEVVAESIERLIIDGVLKVGQPLPSERRLCEKLGFS
                     RSALREGLTVLRGRGIIETAQGRDSRVARLNRVQDTSPLIHLFSTQPRTLYDLLDVRA
                     LLEGESARLAATLGTQADFVVITRCYEKMLAASENNKEISLIEHAQLDHAFHLAICQA
                     SHNQVLVFTLQSLTDLMFNSVFASVNNLYHRPQQKKQIDRQHARIYNAVLQRLPHVAQ
                     RAARDHVRTVKKNLHDIELEGHHLIRSAVPLEMNLS"
     gene            2083..3582
                     /gene="glcD"
                     /locus_tag="ACHOSC_RS17380"
                     /old_locus_tag="pgaptmp_003476"
     CDS             2083..3582
                     /gene="glcD"
                     /locus_tag="ACHOSC_RS17380"
                     /old_locus_tag="pgaptmp_003476"
                     /EC_number="1.1.99.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009339 - glycolate oxidase complex
                     [Evidence IEA];
                     GO_function: GO:0003973 - (S)-2-hydroxy-acid oxidase
                     activity [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycolate oxidase subunit GlcD"
                     /protein_id="HGU4549358.1"
                     /translation="MSILYEERLDGALPDVDRTSVLMALREHVPGLEILHTDEEIIPY
                     ECDGLSAYRTRPLLVVLPKQMEQVTAILAVCHRLRVPVVTRGAGTGLSGGALPLEKGV
                     LLVMARFKEILDINPVGRRARVQPGVRNLAISQAVAPHNLYYAPDPSSQIACSIGGNV
                     AENAGGVHCLKYGLTVHNLLKIEVQTLDGEALTLGSDALDSPGFDLLALFTGSEGMLG
                     VTTEVTVKLLPKPPVARVLLASFDSVEKAGLAVGDIIANGIIPGGLEMMDNLSIRAAE
                     DFIHAGYPVDAEAILLCELDGVESDVQEDCERVNDILLKAGATDVRLAQDEAERVRFW
                     AGRKNAFPAVGRISPDYYCMDGTIPRRALPGVLEGIARLSQQYDLRVANVFHAGDGNM
                     HPLILFDANEPGEFARAEELGGKILELCVEVGGSISGEHGIGREKINQMCAQFNSDEI
                     TTFHAVKAAFDPDGLLNPGKNIPTLHRCAEFGAMHVHHGHLPFPELERF"
     gene            3582..4634
                     /gene="glcE"
                     /locus_tag="ACHOSC_RS17385"
                     /old_locus_tag="pgaptmp_003477"
     CDS             3582..4634
                     /gene="glcE"
                     /locus_tag="ACHOSC_RS17385"
                     /old_locus_tag="pgaptmp_003477"
                     /EC_number="1.1.99.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycolate oxidase subunit GlcE"
                     /protein_id="HGU4549359.1"
                     /translation="MLRECDYSQALLEQVNQAISDKTPLVIQGSNSKAFLGRPVTGQT
                     LDVRCHRGIVNYDPTELVITARAGTPLVAIEAALESAGQMLPCEPPHYGEEATWGGMV
                     ACGLAGPRRPWSGSVRDFVLGTRIITGAGKHLRFGGEVMKNVAGYDLSRLMAGSYGCL
                     GVLTEISMKVLPRPRASLSLRREISLQEAINEIAQWQLQPLPISGLCYFDNALWIRLE
                     GGEGSVKAARELLGGEEVAGQFWQQLREQQLPFFSLPGTLWRISLPSDAPMMDLPGEQ
                     LIDWGGALRWLKSTAEDNQIHRIARNAGGHATRFSAGDGGFAPLSAPLFRYHQQLKQQ
                     LDPCGVFNPGRMYAEL"
     gene            4645..5868
                     /gene="glcF"
                     /locus_tag="ACHOSC_RS17390"
                     /old_locus_tag="pgaptmp_003478"
     CDS             4645..5868
                     /gene="glcF"
                     /locus_tag="ACHOSC_RS17390"
                     /old_locus_tag="pgaptmp_003478"
                     /EC_number="1.1.99.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycolate oxidase subunit GlcF"
                     /protein_id="HGU4549360.1"
                     /translation="MQTQLTEEMRQNARALEADSILRACVHCGFCTATCPTYQLLGDE
                     LDGPRGRIYLIKQVLEGNEVTLKTQEHLDRCLTCRNCETTCPSGVRYHNLLDIGRDIV
                     EQKVKRPLPERMLREGLRQVVPRPAVFRALTQVGLVLRPFLPEQVRAKLPAETVKAKP
                     RPPLRHKRRVLMLEGCAQPTLSPNTNAATARVLDRLGISVMSANEAGCCGAVDYHLNA
                     QEKGLARARNNIDAWWPAIEAGAEAILQTASGCGAFVKEYGQMLKNDALYADKARQVS
                     ELAVDLVELLREEPLEKLAVRGDKKLAFHCPCTLQHAQKLNGEVEKVLLRLGFTLTDV
                     PDSHLCCGSAGTYALTHPDLARQLRDNKMNALESGKPEMIVTANIGCQTHLASAGRTS
                     VRHWIEIVEQALEKE"
     gene            5873..6277
                     /gene="glcG"
                     /locus_tag="ACHOSC_RS17395"
                     /old_locus_tag="pgaptmp_003479"
     CDS             5873..6277
                     /gene="glcG"
                     /locus_tag="ACHOSC_RS17395"
                     /old_locus_tag="pgaptmp_003479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GlcG family protein"
                     /protein_id="HGU4549361.1"
                     /translation="MKTKVILSQQMASAIIAAGQEEAQKNNWSVSIAVADDGGHLLAL
                     SRMDDCAPIAAYISQEKARTAALGRRETKGYEEMVNNGRTAFVTAPLLTSLEGGVPVV
                     VDGQIIGAVGVSGLTGAQDAQVAKAAAAVLAK"
     gene            6299..8470
                     /gene="glcB"
                     /locus_tag="ACHOSC_RS17400"
                     /old_locus_tag="pgaptmp_003480"
     CDS             6299..8470
                     /gene="glcB"
                     /locus_tag="ACHOSC_RS17400"
                     /old_locus_tag="pgaptmp_003480"
                     /EC_number="2.3.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004474 - malate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006097 - glyoxylate cycle [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malate synthase G"
                     /protein_id="HGU4549362.1"
                     /translation="MSQTITQGRLRIDANFKRFVDEEVLPGTGLDAAAFWRNFDEIVH
                     DLAPENRQLLAERDRIQAALDEWHRSNPGPVKDKAAYKSFLRELGYLVPQPECVTVET
                     TGIDSEITSQAGPQLVVPAMNARYALNAANARWGSLYDALYGSDIIPQEGAMVSGYDP
                     QRGEQVIAWVRRFLDESLPLENGSYQDVVAFQVVDKQLRIQLKNGKETTLRTPAQFVG
                     YRGDAAAPTCILLKNNGLHIELQIDANGRIGKDDAAHINDVIVEAAISTILDCEDSVA
                     AVDAEDKILLYRNLLGLMQGTLQEKMEKNGRQIVRKLNDDRQYTAADGSEISLHGRSL
                     LFIRNVGHLMTIPVIWDSEGNEIPEGILDGVMTGAIALYDLKVQKNSRTGSVYIVKPK
                     MHGPQEVAFANKLFTRIETMLGMAPNTLKMGIMDEERRTSLNLRSCIAQARNRVAFIN
                     TGFLDRTGDEMHSVMEAGPMLRKNQMKSTPWIKAYERNNVLSGLFCGLRGKAQIGKGM
                     WAMPDLMADMYSQKGDQLRAGANTAWVPSPTAATLHAMHYHQTNVQSVQANIAQTEFN
                     AEFEPLLDDLLTIPVAENANWSAQEIQQELDNNVQGILGYVVRWVEQGIGCSKVPDIH
                     NVALMEDRATLRISSQHIANWLRHGILTKEQVQASLENMAKVVDQQNADDPAYRPMAG
                     NFANSCAFKAASDLIFLGVKQPNGYTEPLLHAWRLREKESH"
     gene            8698..8927
                     /gene="traT"
                     /locus_tag="ACHOSC_RS17405"
                     /old_locus_tag="pgaptmp_003481"
                     /pseudo
     CDS             8698..8927
                     /gene="traT"
                     /locus_tag="ACHOSC_RS17405"
                     /old_locus_tag="pgaptmp_003481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000850426.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="complement resistance protein TraT"
BASE COUNT         2149 a         2282 c         2549 g         1947 t
ORIGIN      
        1 cactttatcc cgacgatcca aactggatac cccctttatt catagaacgc aacgagcatt
       61 tgtctgcgaa aaatccgggg acggaccata tcatctggca ggcgtgggtg gcaaaaaaag
      121 cggggcagat agttgggcgt attaccgcgc aaatagatac cttgcaccgt gagcgttacg
      181 gtgaagatac cggtcatttc ggcatgattg acgccattga tgatccgcag gtttttgccg
      241 cgttgtttgg cgcagcggaa gcgtggttga agtcacaagg tgcaagtaag atcagcggtc
      301 ctttcagcct gaatatcaat caggaaagcg gattactgat tgaaggtttt gacacaccac
      361 cctgtgcgat gatgccacac ggcaaaccgt ggtatgccgc gcatattgaa caactgggtt
      421 atcacaaagg cattgattta ctggcgtggt ggatgcagcg aaccgatctc actttctctc
      481 cggcgctaaa aaaactgatg gatcaggtgc gcaaaaaggt gaccattcgc tgcatcaatc
      541 gtcagcggtt tgccgaagaa atgcagatcc tgcgtgagat tttcaactct ggctggcagc
      601 acaactgggg atttgtgccg tttaccgaac atgaattcgc gaccatgggc gatcaactta
      661 aatatctggt gccggacgat atgatctata tcgctgagat tgattctgca ccctgcgcgt
      721 ttattgtcgg cttgccgaac atcaacgagg cgattgccga tctgaacgga tcgctctttc
      781 ccttcggctg ggcaaaattg ctgtggcgtt tgaaagtcag cggtgtgcga actgcgcgag
      841 taccgctgat gggcgtgcgc gacgagtatc agttcagccg catcggcccg gtgattgcgc
      901 tgttattgat tgaagcctta cgcgatccgt ttgcccgccg gaagatcgat gcgctggaga
      961 tgtcatggat cctcgaaacc aatacaggca tgaataacat gctggaacgc attggggcgg
     1021 agccgtacaa gcgttaccga ttgtatgaaa agcagattta ataccagcta actcaggttc
     1081 atctccagcg gcaccgccga gcgaatcaaa tggtggcctt ccagctcgat atcgtggaga
     1141 ttctttttca cggtccgcac atgatcgcgt gctgcgcgct gggcgacgtg cggcagccgc
     1201 tgcaacaccg cgttgtagat cctcgcatgc tggcgatcga tctgcttttt ttgctgtggt
     1261 cgatggtaga gattatttac gctggcaaac actgaattaa acatcagatc ggtcaatgat
     1321 tgcagcgtaa acaccagcac ctgattgtga gaagcctgac aaatggcgag atggaaagcg
     1381 tgatccaact gcgcatgttc gatcagcgaa atctctttgt tgttctcact ggcggcgagc
     1441 attttttcat aacagcgggt tatcacaaca aaatcagcct gcgttcccag cgttgccgcc
     1501 agccttgccg attcgccctc cagtaatgcg cgaacgtcga gcagatcgta cagcgttcgc
     1561 ggctgcgtac tgaacagatg gatcagcgga ctggtgtcct gcacccgatt aagccgtgcg
     1621 acacgagaat cgcgaccctg cgccgtttca ataatcccgc gcccgcgcag cacggtcagc
     1681 ccttcacgca gtgcggagcg tgagaagccg agcttttcac acagtcgacg ttccgaggga
     1741 agtggctgac cgaccttcag tacgccgtcg ataattaacc gttcgatact ctctgcaacc
     1801 acttcgcaaa tagggcgacg ttcatctttc attcccggac ctcgtgcaca ggtaggacca
     1861 atttttctgt tcttaactcg caaaacacgc acatcacgta agtgattatg ttacatcaat
     1921 ttaacattga gttaaccaag gcaaggtcac agagctggaa aaaaaatggt ctgaccggta
     1981 gctaaagaga tagacgaaaa cgaaaagccc gcttaataac tgttcacaga agcagcgcgc
     2041 aaaaatcagc tgccacacaa cacaacaaag cgaagcctac tcatgagcat cttgtacgaa
     2101 gagcgtcttg atggcgcttt acccgatgtc gaccgcacat cggtactgat ggcactgcgt
     2161 gagcatgtcc ctggacttga gatcctgcat accgatgagg agatcattcc ttacgagtgt
     2221 gacgggttga gcgcgtatcg cacgcgtcca ttactggttg ttctgcctaa gcaaatggaa
     2281 caggtgacag cgattcttgc tgtctgccat cgcctgcgtg taccggtggt gacccgtggt
     2341 gcaggcaccg ggctttctgg tggcgcgctg ccgctggaaa aaggtgtgtt gttggtgatg
     2401 gcgcgcttta aagagatcct cgacattaac cccgttggtc gccgcgcgcg cgtgcagcca
     2461 ggcgtgcgta acctggcgat ctcccaggcc gttgcaccgc ataatctcta ctacgcaccg
     2521 gacccttcct cacaaatcgc ctgttccatt ggcggcaacg tggcagaaaa tgccggcggc
     2581 gtccactgcc tgaaatatgg tctgaccgta cataacctgc tgaaaattga agtgcaaacg
     2641 ctggacggcg aggcgctgac gctgggatcg gacgcgctgg attcacctgg ttttgacctg
     2701 ctggcgctgt tcaccggatc ggaaggtatg ctcggcgtga ccaccgaagt gacggtaaaa
     2761 ctgctgccga agccgcccgt ggcgcgggtt ctgttagcca gctttgactc ggtagaaaaa
     2821 gccggacttg cagttggtga catcatcgcc aatggcatta tccccggcgg gctggagatg
     2881 atggataacc tgtcgatccg cgcggcggaa gattttattc atgccggtta tcccgtcgac
     2941 gccgaagcga ttttgttatg cgagctggac ggcgtggagt ctgacgtaca ggaagactgc
     3001 gagcgggtta acgacatctt gttgaaagcg ggcgcgactg acgtccgtct ggcacaggac
     3061 gaagcagagc gcgtacgttt ctgggccggt cgcaaaaatg cgttcccggc ggtaggacgt
     3121 atctccccgg attactactg catggatggc accatcccgc gtcgcgccct gcctggcgta
     3181 ctggaaggca ttgcccgttt atcgcagcaa tatgatttac gtgttgccaa cgtctttcat
     3241 gccggagatg gcaacatgca cccgttaatc cttttcgatg ccaacgaacc cggtgaattt
     3301 gcccgcgcgg aagagctggg tgggaagatc ctcgaactct gcgttgaagt tggcggcagc
     3361 atcagtgggg aacatggcat cgggcgagaa aaaatcaatc aaatgtgcgc ccagttcaac
     3421 agcgatgaaa tcacgacctt ccatgcggtc aaggcggcgt ttgaccccga tggtttgctg
     3481 aaccctggga aaaacattcc cacgctacac cgctgtgctg aatttggtgc catgcatgtg
     3541 catcacggtc atttaccttt ccctgaactg gagcgtttct gatgctacgc gagtgtgatt
     3601 acagccaggc gctgctggag caggtgaatc aggcaattag cgataaaacg ccgctggtga
     3661 ttcagggcag caatagcaaa gcctttttag gtcgccctgt caccgggcaa acgctggatg
     3721 tgcgttgtca tcgcggcatt gttaattacg acccgaccga gctggtgata accgcgcgtg
     3781 ccggaacgcc gctggtggcg attgaagcgg cgctggagag cgccgggcaa atgctcccct
     3841 gcgagccgcc gcattatggt gaagaagcca cctggggcgg gatggtcgcc tgcgggctgg
     3901 cggggccgcg tcgcccgtgg agcggttcgg tccgcgattt tgtcctcggc acgcgcatca
     3961 ttaccggcgc tggaaaacat ctgcgttttg gtggcgaagt gatgaaaaac gttgccggat
     4021 acgatctctc acggttaatg gccggaagct acggttgtct tggcgtgctc actgaaatct
     4081 cgatgaaagt gttaccgcga ccgcgcgcct ccctgagcct gcgtcgggaa atcagcctgc
     4141 aagaagccat taacgaaatc gcccagtggc aactccagcc gctacccatt agtggcttat
     4201 gttacttcga caatgcgttg tggatccgcc ttgagggcgg cgaaggatcg gtaaaagcgg
     4261 cgcgtgaact gctggggggc gaagaggttg cgggtcagtt ctggcagcaa ttgcgtgaac
     4321 aacaactgcc gttcttctcg ttaccaggta ccttatggcg catttcatta cccagcgatg
     4381 cgccgatgat ggatttaccc ggcgagcaac tgatcgactg gggcggagcg ttacgctggc
     4441 tgaaatcgac agccgaggac aatcaaatcc atcgcatcgc ccgcaacgct ggcggtcatg
     4501 cgacccgctt tagtgccgga gatggtggct ttgccccgct atcggctcct ttattccgct
     4561 atcaccagca gcttaaacag cagctcgacc cttgcggcgt gtttaacccc ggtcgcatgt
     4621 acgcggaact ttgaggagca ggctatgcaa acccaattaa ctgaagagat gcggcagaac
     4681 gcgcgcgcgc tggaagccga cagcatcctg cgcgcctgtg ttcactgcgg attttgtacc
     4741 gcaacctgcc caacctatca gcttctgggc gatgaactgg acgggccgcg cgggcgcatc
     4801 tatctgatta aacaggtgct ggaaggcaac gaagtcacgc ttaaaacaca ggagcatctc
     4861 gatcgctgcc tcacttgccg caattgtgaa accacctgtc cttctggtgt gcgctatcac
     4921 aatttgctgg atatcgggcg tgatattgtc gagcagaaag tgaaacgccc actgccggag
     4981 cgaatgctgc gcgaaggatt gcgccaggta gtgccgcgtc cggcggtctt ccgtgcgctg
     5041 acgcaggtag ggctggtgct gcgaccgttt ttaccggaac aggtcagagc aaaactgcct
     5101 gctgaaacgg tgaaagctaa accgcgtccg ccgctgcgcc ataagcgtcg ggttttaatg
     5161 ctggaaggct gcgcccagcc tacgctttcg cccaacacca acgcggcaac tgcgcgagtg
     5221 ctggatcgtc tggggatcag cgtcatgtca gctaacgaag caggctgttg tggcgcggtg
     5281 gactatcatc ttaatgcgca ggagaaaggg ctggcacggg cgcgcaataa tattgatgcc
     5341 tggtggcccg cgattgaagc aggtgccgag gcaattttgc aaaccgccag cggttgcggc
     5401 gcgtttgtca aagagtacgg gcagatgctg aaaaacgatg cgttatatgc cgataaagcg
     5461 cgtcaagtca gtgaactggc ggtcgattta gtcgagcttc tgcgcgagga accgctggaa
     5521 aaactggcag ttcgcggcga taaaaaactg gccttccact gcccgtgtac cctacaacat
     5581 gcgcaaaagc tgaacggcga agtggaaaaa gtgttgcttc gtcttggatt taccttaacg
     5641 gacgttcccg acagccatct gtgctgcggt tcagcgggaa catatgcgtt aacgcatccc
     5701 gatctggcac gccagctgcg ggataacaaa atgaatgcgc tggaaagcgg caaaccggaa
     5761 atgatcgtca ccgccaacat tggttgccag acgcatctgg cgagcgccgg tcgtacctca
     5821 gtgcgtcact ggattgaaat tgtagaacaa gcccttgaaa aggaataaaa aaatgaaaac
     5881 taaagtcatt cttagccagc aaatggcgag tgcaattatt gccgcaggtc aggaagaggc
     5941 gcagaaaaat aactggtcgg tttccattgc tgttgccgat gacggcggtc atctgctggc
     6001 gttaagtcgc atggacgatt gcgcgccgat tgcggcttac atctcccagg agaaagcgcg
     6061 taccgccgcg ctggggcgtc gtgaaactaa gggctatgaa gagatggtga acaacggacg
     6121 taccgcgttc gtgactgcgc cgttactgac gtcgctggaa ggcggcgtac cggttgttgt
     6181 ggatgggcaa attattggtg ccgtgggcgt ttctggttta accggagcac aggatgcgca
     6241 ggtcgcgaaa gcggcagcag cggtgttggc gaaataagcg aaaacgagga gagaaacaat
     6301 gagtcaaacc ataacccagg gccgtttacg cattgacgcc aattttaaac gttttgtgga
     6361 tgaagaagtt ttaccgggaa cagggctgga cgctgcggcg ttctggcgca attttgatga
     6421 gatcgttcac gatctggcac cagaaaatcg tcagttgctg gcagaacgcg atcgcattca
     6481 ggcagcgctt gatgagtggc atcgcagcaa tccggggccg gtaaaagata aagcggccta
     6541 taaatctttc ctgcgtgaac tgggctacct ggtgccgcaa ccggagtgcg tgacggtgga
     6601 aaccacgggc attgacagcg aaatcaccag tcaggcaggg ccacagctgg tggttccggc
     6661 aatgaacgcc cgctacgcgc tgaacgcggc gaacgctcgc tggggctcac tgtacgatgc
     6721 gttatacggc agcgacatca tcccgcagga aggggcgatg gtcagcggct acgatccgca
     6781 gcgcggtgag caggttatcg cctgggttcg gcgtttcctc gatgaatctc taccgctgga
     6841 aaacggcagc tatcaggatg tggtggcgtt tcaggtggtc gataaacaat tacgcatcca
     6901 gttgaaaaat ggtaaagaaa ccacgttacg taccccggcg cagtttgtcg gttaccgtgg
     6961 cgatgccgct gcgccgacct gcattttgct gaaaaataac ggcctgcata ttgaactgca
     7021 aattgatgcc aacggacgga ttgggaaaga cgatgcggcg cacatcaacg atgttatcgt
     7081 cgaagctgca atcagtacca ttctcgactg cgaagattcg gtcgcggcgg ttgatgcgga
     7141 agataaaatc ctgctgtacc gcaatctgct gggcctgatg caggggactc tccaagagaa
     7201 aatggagaaa aacggtcgac aaatcgtgcg taaactgaat gacgatcgtc agtacaccgc
     7261 cgccgatggc tctgaaattt ctctgcacgg acgctcgctg ctgtttatcc gcaacgtggg
     7321 tcatttgatg accattcctg tgatttggga cagcgaaggc aatgaaatcc cggaaggcat
     7381 tcttgatggc gtcatgactg gcgcgattgc cctctatgat ttaaaagtgc agaaaaactc
     7441 gcgcactggc agcgtctata ttgtgaaacc gaaaatgcac ggtccgcagg aagtggcgtt
     7501 cgccaacaaa ctgtttaccc gcattgagac aatgctcggt atggcaccga ataccctgaa
     7561 aatgggcatt atggatgaag aacgtcggac ctcgctgaac ttgcgtagct gtatcgctca
     7621 ggcacgcaac cgcgtggcgt tcatcaatac cggtttcctc gaccgtaccg gcgatgaaat
     7681 gcattcggta atggaagctg gcccgatgct gcgtaaaaat cagatgaaat cgacgccgtg
     7741 gatcaaagcc tacgagcgta ataacgtgct ttccggcctg ttctgtgggc tgcgcggtaa
     7801 agcgcaaatc ggtaaaggca tgtgggcaat gccggacctg atggcagaca tgtacagcca
     7861 gaagggcgac caactgcgtg ccggggcaaa cacagcctgg gttccgtcgc caactgccgc
     7921 tacgctccat gcgatgcact accaccaaac caacgtacag agcgtgcaag ccaacattgc
     7981 ccagactgag ttcaatgctg aatttgaacc gctgctggac gatctgctga ctattccggt
     8041 tgctgaaaac gctaactggt cggcgcaaga gatccaacaa gagctggata acaacgtgca
     8101 ggggattctg gggtacgtgg tgcgctgggt ggagcagggg attggttgtt caaaagtgcc
     8161 ggatattcac aatgtggcgc tgatggaaga ccgcgcaacg ctgcgtatct ccagccagca
     8221 tatcgccaac tggttacgtc acggtattct gaccaaagaa caggtgcagg cgtcgctgga
     8281 gaatatggcg aaagtggttg atcagcaaaa cgctgacgat ccggcttatc gtccgatggc
     8341 ggggaatttc gctaactcgt gtgcttttaa agctgccagc gatttaatct tcctcggcgt
     8401 gaaacagcca aacggctata ccgaaccgtt attacacgcc tggcgtttac gcgaaaaaga
     8461 aagtcattaa tagcgtcaaa aaaagccccg gcaagtttta ttgtcggggc tatgatagta
     8521 actgcggtag tacatttatc ccggaaataa ccggagcgat tatcagggtt taacaatatt
     8581 aaatacccgt agcgcattac tagcgggcct ataacaacaa tatggacatc ttaacgatac
     8641 tctgcgcctg cgtgtgcaga acagaaaccc ttacccgata tacttgttat gccagaggta
     8701 gcaattgcgg caaccgatct gactggcatg gatgcagatg caatggtgga agatataaat
     8761 tacacgataa ttacagatgt tcagattacc gaacgaacta aaacctatgt cagagcagat
     8821 aatgctacca cttttcggca gggaacatca ggcaccgaaa ttcaggctag cacagaaacc
     8881 agcaaccagg ttaatctgaa atttgaaggg gctaaacctc ttctgga
//
LOCUS       DAZKHF020000183         8865 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2201S31241
            SAMN43267800-rid25106413.denovo.184, whole genome shotgun sequence.
ACCESSION   DAZKHF020000183 DAZKHF020000000
VERSION     DAZKHF020000183.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267800
            Sequence Read Archive: SRR30307263
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 8865)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8865)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 8865)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350581.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350581.2
            Genome Coverage       :: 151x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:21:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,652
            CDSs (total)                      :: 5,557
            Genes (coding)                    :: 5,320
            CDSs (with protein)               :: 5,320
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 237
            CDSs (without protein)            :: 237
            Pseudo Genes (ambiguous residues) :: 0 of 237
            Pseudo Genes (frameshifted)       :: 102 of 237
            Pseudo Genes (incomplete)         :: 130 of 237
            Pseudo Genes (internal stop)      :: 52 of 237
            Pseudo Genes (multiple problems)  :: 41 of 237
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8865
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267800-rid25106413.denovo.184"
                     /serotype="O 26:H11"
                     /isolate="2201S31241"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-01"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            <1..293
                     /gene="rcsA"
                     /locus_tag="ACHOSC_RS17410"
                     /old_locus_tag="pgaptmp_003482"
     CDS             <1..293
                     /gene="rcsA"
                     /locus_tag="ACHOSC_RS17410"
                     /old_locus_tag="pgaptmp_003482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="transcriptional regulator RcsA"
                     /protein_id="HGU4549363.1"
                     /translation="SLDDILGDILKKETTITSFLNMPTLSLSRTESSMLRMWMAGQGT
                     IQISDQMNIKAKTVSSHKGNIKRKIKTHNKQVIYHVVRLTDNVTNGIFVNMR"
     gene            complement(337..525)
                     /gene="dsrB"
                     /locus_tag="ACHOSC_RS17415"
                     /old_locus_tag="pgaptmp_003483"
     CDS             complement(337..525)
                     /gene="dsrB"
                     /locus_tag="ACHOSC_RS17415"
                     /old_locus_tag="pgaptmp_003483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein DsrB"
                     /protein_id="HGU4549364.1"
                     /translation="MKVNDRVTVKTDGGPRRPGVVLAVEEFSEGTMYLVSLEDYPLGI
                     WFFNEAGHQDGIFVEKAE"
     gene            688..915
                     /gene="yodD"
                     /locus_tag="ACHOSC_RS17420"
                     /old_locus_tag="pgaptmp_003484"
     CDS             688..915
                     /gene="yodD"
                     /locus_tag="ACHOSC_RS17420"
                     /old_locus_tag="pgaptmp_003484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416463.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxide/acid resistance protein YodD"
                     /protein_id="HGU4549365.1"
                     /translation="MKTAKEYSDTAKREVSVDVDALLAAINEISESEVHRSQNDSEHV
                     SVDGREYHTWRELADAFELDIHDFSVSEVNR"
     gene            1213..2028
                     /gene="yedP"
                     /locus_tag="ACHOSC_RS17425"
                     /old_locus_tag="pgaptmp_003485"
     CDS             1213..2028
                     /gene="yedP"
                     /locus_tag="ACHOSC_RS17425"
                     /old_locus_tag="pgaptmp_003485"
                     /EC_number="3.1.3.70"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000949103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050531 - mannosyl-3-phosphoglycerate
                     phosphatase activity [Evidence IEA];
                     GO_process: GO:0051479 - mannosylglycerate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannosyl-3-phosphoglycerate phosphatase-related
                     protein"
                     /protein_id="HGU4549366.1"
                     /translation="MLSIQQPLLVFSDLDGTLLDSHSYDWQPAAPWLSRLREANVPVI
                     LCSSKTSAEMLYLQKTLGLQGLPLIAENGAVIQLAEQWQDIDGFPRIISGISHGEISQ
                     VLNTLREKEHFKFTTFDDVDDATIAEWTGLSRSQAALTQLHEASVTLIWRDSDERMAQ
                     FTARLNELGLQFMQGARFWHVLDASAGKDQAANWIIATYQQSSGKRPTTLGLGDGPND
                     APLLEVMDYAVIVKGLNREGVHLHDEDPARVWRTQREGPEGWREGLDHFFSAH"
     gene            complement(2025..3719)
                     /gene="dgcQ"
                     /locus_tag="ACHOSC_RS17430"
                     /old_locus_tag="pgaptmp_003486"
     CDS             complement(2025..3719)
                     /gene="dgcQ"
                     /locus_tag="ACHOSC_RS17430"
                     /old_locus_tag="pgaptmp_003486"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416465.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose biosynthesis regulator diguanylate
                     cyclase DgcQ"
                     /protein_id="HGU4549367.1"
                     /translation="MQHETKMENQSWLKKLARRLGPGHVVNLCFIVVLLFSTLLTWRE
                     VVVLEDAYISSQRNHLENVANALDKHLQYNVDKLIFLRNGMREALVAPLDFTSLRNAV
                     TEFEQHRDEHAWQIELNRRRTLPVNGVSDALVSEGNLLSRENESLDNEITAALEVGYL
                     LRLAHNSSSMVEQAMYVSRAGFYVSTQPTLFTRNVPTRYYGYVTQPWFVGHSQRENRH
                     RAVRWFTSQPEHASNTEPQVTVSVPVDSENYWYGVLGMSIPVRTMQQFLRNAIDKNLD
                     GEYQLYDSKLRLLTSSNPDHPTGNIFDPRELALLAQAMEHDTRGGIRMNSRYVSWERL
                     DHFDGVLVRVHTLSEGVRGDFGSISIALTLLWALFTTMLLISWYVIRRMVSNMYVLQS
                     SLHWQAWHDTLTRLYNRGALFEKARPLAKLCQTHQHPFSVIQVDLDHFKAINDRFGHQ
                     AGDRVLSHAAGLISSSLRAQDVAGRVGGEEFCVILPGASLTQAAEVAERIRLKLNEKE
                     MLIAKSTTIRISASLGVSSSEETGDYDFEQLQSLADRRLYLAKQAGRNRVFASDNA"
     gene            complement(3957..4139)
                     /gene="yodC"
                     /locus_tag="ACHOSC_RS17435"
                     /old_locus_tag="pgaptmp_003487"
     CDS             complement(3957..4139)
                     /gene="yodC"
                     /locus_tag="ACHOSC_RS17435"
                     /old_locus_tag="pgaptmp_003487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2158 domain-containing protein"
                     /protein_id="HGU4549368.1"
                     /translation="MSFMVSEEVTVKEGGPRMIVTGYSSGMVECRWYDGYGVKREAFH
                     ETELVPGEGSRSAEEV"
     gene            complement(4218..5135)
                     /gene="yedI"
                     /locus_tag="ACHOSC_RS17440"
                     /old_locus_tag="pgaptmp_003488"
     CDS             complement(4218..5135)
                     /gene="yedI"
                     /locus_tag="ACHOSC_RS17440"
                     /old_locus_tag="pgaptmp_003488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF808 domain-containing protein"
                     /protein_id="HGU4549369.1"
                     /translation="MLLAGSSLLTLLDDIATLLDDISVMGKLAAKKTAGVLGDDLSLN
                     AQQVAGVRANRELPVVWGVAKGSLINKVILVPLALIISAFIPWAITPLLMIGGAFLCF
                     EGVEKVLHMLEARKHKEDPAQSQQRLEKLAAQDPLKFEKDKIKGAIRTDFILSAEIVA
                     ITLGIVAEAPLLNQVLVLSGIALVVTVGVYGLVGVIVKIDDLGYWLAEKSSALMQALG
                     KGLLIIAPWLMKALSIVGTLAMFLVGGGIVVHGIAPLHHAIEHFAGQQSAVVAMILPT
                     VLNLILGFIIGGIVVLGVKAVAKIRGQAH"
     gene            5308..6228
                     /gene="yedA"
                     /locus_tag="ACHOSC_RS17445"
                     /old_locus_tag="pgaptmp_003489"
     CDS             5308..6228
                     /gene="yedA"
                     /locus_tag="ACHOSC_RS17445"
                     /old_locus_tag="pgaptmp_003489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="drug/metabolite exporter YedA"
                     /protein_id="HGU4549370.1"
                     /translation="MRFRQLLPLFGALFALYIIWGSTYFVIRIGVESWPPLMMAGVRF
                     LAAGILLLAFLLLRGHKLPPLRPLLNAALIGLLLLAVGNGMVTVAEHQNVPSGIAAVV
                     VATVPLFTLCFSRLFGIKTRKLEWVGIAIGLAGIIMLNSGGNLSGNPWGAILILIGSI
                     SWAFGSVYGSRITLPVGMMAGAIEMLAAGVVLMIASMIAGEKLTALPSLSGFLAVGYL
                     ALFGSIIAINAYMYLIRNVSPALATSYAYVNPVVAVLLGTGLGGETLSKIEWLALGVI
                     VFAVVLVTLGKYLFPAKPVVAPVIPDASSE"
     gene            complement(6217..6687)
                     /gene="vsr"
                     /locus_tag="ACHOSC_RS17450"
                     /old_locus_tag="pgaptmp_003490"
     CDS             complement(6217..6687)
                     /gene="vsr"
                     /locus_tag="ACHOSC_RS17450"
                     /old_locus_tag="pgaptmp_003490"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VSPR family DNA mismatch endonuclease"
                     /protein_id="HGU4549371.1"
                     /translation="MVDVHDKATRSKNMRAIATRDTAIEKRLASLLTGQGLAFRVQDA
                     SLPGRPDFVLDEFRCVIFAHGCFWHHHHCYLFKVPATRTDFWLEKIGKNVERDRRDIS
                     HLQELGWRVLIVWECALRGREKLTDAALTERLEEWICGEGASAQIDTQGIHLLA"
     gene            complement(6668..8086)
                     /gene="dcm"
                     /locus_tag="ACHOSC_RS17455"
                     /old_locus_tag="pgaptmp_003491"
     CDS             complement(6668..8086)
                     /gene="dcm"
                     /locus_tag="ACHOSC_RS17455"
                     /old_locus_tag="pgaptmp_003491"
                     /EC_number="2.1.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-cytosine methyltransferase"
                     /protein_id="HGU4549372.1"
                     /translation="MQENISVTDSYSTGNAAQAMLEKLLQIYDVKTLVAQLNGVGENH
                     WSAAILKRALANDSAWHRLSEKEFAHLQTLLPKPPAHHPHYAFRFIDLFAGIGGIRRG
                     FESIGGQCVFTSEWNKHAVRTYKANHYCDPATHHFNEDIRDITLSHQEGVSDEAAAEH
                     IRQHIPEHDVLLAGFPCQPFSLAGVSKKNSLGRAHGFACDTQGTLFFDVVRIIDARRP
                     AMFVLENVKNLKSHDQGKTFRIIMQTLDELGYDVADAEDNGPDDPKIIDGKHFLPQHR
                     ERIVLVGFRRDLNLKADFTLRDISKCFPAQRVTLAQLLDPMVEAKYILTPVLWKYLYR
                     YAKKHQARGNGFGYGMVYPNNPQSVTRTLSARYYKDGAEILIDRGWDMATGEKDFDDP
                     LNQQHRPRRLTPRECARLMGFEAPGEAKFRIPVSDTQAYRQFGNSVVVPVFAAVAKLL
                     EPKIKQAVALRQQEAQHGRRSR"
     gene            complement(8153..8590)
                     /locus_tag="ACHOSC_RS17460"
                     /old_locus_tag="pgaptmp_003492"
                     /pseudo
     CDS             complement(8153..8590)
                     /locus_tag="ACHOSC_RS17460"
                     /old_locus_tag="pgaptmp_003492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416471.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
     gene            8646..>8865
                     /locus_tag="ACHOSC_RS17465"
                     /old_locus_tag="pgaptmp_003493"
     CDS             8646..>8865
                     /locus_tag="ACHOSC_RS17465"
                     /old_locus_tag="pgaptmp_003493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_086558153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS1 family transposase"
                     /protein_id="HGU4549373.1"
                     /translation="MASISIRCPSCSATEGVVRNGKSTAGHQRYLCSHCRKTWQLQFT
                     YTASQPGKHQKIIDMAMNGVGCRASARIM"
BASE COUNT         2122 a         2395 c         2183 g         2165 t
ORIGIN      
        1 aatctctcga cgatatcctt ggcgatattc tgaaaaaaga gacaacgata acctcgtttt
       61 taaatatgcc gacgttatca ttgagccgaa ccgaatcgag tatgttgcga atgtggatgg
      121 caggtcaggg aaccattcaa atctctgacc aaatgaatat caaagccaag accgtttcat
      181 cgcataaagg taatattaaa cgtaagatca aaacgcataa taaacaggtt atctaccatg
      241 tcgtccgact gacggataat gtgactaatg gtatttttgt caacatgcgc taacacattc
      301 tgactggtgg tttcccacca gtcaggccga attagattac tctgctttct ccacaaagat
      361 accgtcctga tgccctgctt cattaaagaa ccagatgccg agcgggtagt cttccagcga
      421 aaccaggtac attgtgcctt cactaaactc ctcaactgcc agtaccacgc caggacgacg
      481 cggaccgcca tccgttttga ctgttacccg atcattcacc ttcatcgttt tcctcctgtg
      541 gctttgtgcc agtgtagaac aatttcgttt ttctggcagc gccaggcgcg cgcgagtgct
      601 gattttctcg acggtctata cttaagagat gccaggcgga cttaacgact ggccgcaaca
      661 acagagtaac ggttgcgagg aaagatgatg aaaaccgcaa aagagtacag cgataccgca
      721 aaacgtgagg tcagcgtcga tgtcgatgcc ctgctggcgg cgatcaatga aattagcgaa
      781 agcgaagttc atcgcagcca gaacgattct gaacacgtta gcgtcgatgg acgtgaatat
      841 catacatggc gtgaattggc ggatgccttc gaactggata ttcatgactt cagcgtctct
      901 gaagtgaatc gttgaatgca caataaaaaa atcccgaccc tgagggggtc gggatgaaac
      961 ttgcttaagc aagaagcact taaaaaattc gttacaccag gaaatctgat gtgttcatca
     1021 ccttatccgc aatttttttc gctgacaaga aaatattcgt gatatgaatg attaacgcta
     1081 cttatgaata gaaatgtgac gcgcgtcacc ccttggtggc tctggcgtca acgggatcgc
     1141 tcccgaaaaa atcatctttg ataatttgtg ctgttttgag aatcatcctg gtaaggggag
     1201 tactgcgcaa ccatgctttc aattcaacaa ccactactgg tttttagcga tcttgatggc
     1261 accctgctgg acagtcatag ttatgactgg caaccagctg ccccctggct cagccgttta
     1321 cgcgaagcga atgttcccgt cattctctgt agcagtaaga catcagccga aatgctgtac
     1381 ttgcaaaaaa cgttggggct acaaggttta ccgctgattg ccgaaaatgg cgcagtgatc
     1441 cagcttgctg agcaatggca ggatatagac ggttttccac gcatcatctc aggtattagc
     1501 catggcgaaa tcagccaggt tttaaatacg ctacgcgaga aagaacattt taaattcaca
     1561 acttttgatg atgtcgacga tgcaaccatc gccgaatgga cgggattaag ccgtagccag
     1621 gcggcgctga cgcagctaca tgaggcgtcg gtaacgctaa tctggcgcga cagtgacgag
     1681 cgtatggcac aatttaccgc tcgtctgaac gaactgggct tgcagtttat gcagggtgcg
     1741 cgcttctggc acgtcctgga tgcctctgcc ggaaaagatc aggctgccaa ctggattatc
     1801 gcgacctatc aacaatcgtc aggcaaacgc ccaaccacac ttggcctggg cgatgggcca
     1861 aacgatgcgc ccttactgga ggtaatggat tacgcggtga ttgtgaaagg gttaaaccgt
     1921 gaaggggtgc atctgcatga tgaggatccg gcccgcgtct ggcgaacgca gcgtgaagga
     1981 ccggaaggct ggcgtgaagg gctggaccat tttttctccg cccattaagc gttatcgctc
     2041 gcgaataccc gattgcgccc ggcctgttta gcgagataaa gtcgacggtc ggccagtgac
     2101 tggagttgtt caaaatcata atcaccggtt tcctcgctgc tgcttacccc cagcgaggca
     2161 ctgatgcgta tcgtcgtact cttggcgatc aacatctctt tttcatttag cttcaggcga
     2221 atacgttctg caacctccgc agcctgcgtc aggctcgcgc ctggcagaat cacacaaaac
     2281 tcctcaccac cgacccgccc ggcaacatcc tgcgctcgca aggaactgct aattaatccg
     2341 gcagcatgag aaagaacacg gtcgcccgcc tgatgaccaa agcggtcatt aatcgcttta
     2401 aaatggtcaa ggtcgacctg aatgacagaa aaaggatgtt ggtgcgtctg acacaattta
     2461 gcgagcggac gggctttttc gaacagtgcg ccacggttat ataaacgcgt taaggtgtcg
     2521 tgccacgcct gccagtgcaa cgagctttgc aaaacataca tgttgctgac catccggcga
     2581 atcacatacc aggagatgag taacatggtg gtaaagagcg cccacagcag ggttaatgca
     2641 atgctgatac tgccgaaatc gccgcgcacg ccttcgctta gcgtatggac gcgcaccagc
     2701 acaccgtcga aatggtccag acgttcccag ctaacatagc gactgttcat acgaatgccg
     2761 ccccgcgtgt catgttccat tgcctgcgcc agcaaggcca gttcacgagg atcaaaaata
     2821 ttccctgttg gatggtcagg attggaagag gtcaataatc tcagcttact gtcatagagc
     2881 tgatactcac catcgaggtt tttatcgatg gcgtttctta aaaattgctg catggtacgc
     2941 acgggaatac tcatccccag cacgccatac cagtagtttt cactgtctac cggaacactg
     3001 acggtaacct gcggttcagt attgctggcg tgttccggtt gcgaagtgaa ccagcgtacc
     3061 gcgcggtgac gattttctcg ttgcgaatgg ccgacaaacc agggttgggt gacatagccg
     3121 taataacgcg ttggtacatt gcgcgtaaac aaggtcggct gcgtcgaaac gtaaaatccg
     3181 gcacgcgaga catacatcgc ctgttcaacc atcgacgagg agttgtgtgc cagtcgcagc
     3241 aagtaaccaa cttccagtgc agcggtaatt tcattgtcga ggctttcatt ttcgcgagac
     3301 aggagattcc cctcgctgac taatgcatcc gacacaccat tgactggcag ggtgcgtcgt
     3361 cggttgagtt cgatttgcca ggcgtgctcg tcgcgatgct gttcgaactc ggttacggca
     3421 ttacgcagag aggtgaaatc cagtggcgct acgagagctt cgcgcatgcc attacgcaaa
     3481 aagatcagtt tgtcgacgtt atactgcaaa tgcttatcga gcgcgttggc tacgttttcc
     3541 agatgattac gctggctgga gatataggca tcttccagca ccaccacttc acgccaggtg
     3601 agcaaggtgg aaaaaagcaa taccacgata aagcagagat taacgacatg accaggcccc
     3661 aggcggcgtg cgagtttttt caaccagctc tggttttcca tttttgtctc gtgctgcacc
     3721 ctgacgactc ccaacctgct tttttatgat tctggcataa cctggcgata gcgtaccgca
     3781 aaacagcgat aattttataa tggtggcatg ataagacgcg acagacatcg atagttttat
     3841 aacgtaggca tgataagacg cgatagcgtc gcatcaggca attcagtgca gacactggca
     3901 cacccgaccg gatgcgacgt gagcgccaca tccggtcggg tgttaatgat accctttcaa
     3961 acttcttccg cagaacgact cccctccccc ggaacaagct cggtttcatg aaaagcttcc
     4021 cgcttgaccc cgtaaccgtc ataccaacga cactcaacca taccgctgga gtatccggtg
     4081 acaatcatcc gcgggccgcc ctctttaacc gtaacttcct cactaaccat aaagctcata
     4141 ctcgcctcct tttttcgagt gaaacttgtt caccttagtt gaagatggcg aattttgcat
     4201 aatagcctgt gcgagtatta atgtgcctga ccgcgtattt tcgctacggc tttcactccc
     4261 agcaccacga tgccgccgat gataaatcca agaatcagat ttaaaacagt cggtaatatc
     4321 atcgccacca ctgcactttg ctgcccggcg aaatgttcaa tggcgtgatg cagcggcgca
     4381 attccatgta ccacaatccc accgccgacg aggaacatcg ccagcgtgcc gacaatcgat
     4441 aacgccttca tcaaccaggg cgcaatgatc aataaccctt tgcctaatgc ctgcatcagc
     4501 gcgctggatt tttccgccag ccaatacccc aggtcatcaa tcttaacgat aacccctacc
     4561 agaccgtaga cgcccacggt cactaccagc gcgatgcctg aaagcaccag cacctgatta
     4621 agcaacggcg cttcggccac aatccccagc gtgatggcga cgatttccgc agacaatata
     4681 aaatcggtac gaatcgcccc ttttattttg tctttttcaa acttcagcgg atcctgcgcc
     4741 gccagcttct ccagacgctg ctgactctgc gccggatctt ctttatgttt acgcgcctcc
     4801 agcatatgca gcactttctc taccccttca aagcagagaa acgcgccgcc aatcatcaac
     4861 agtggcgtaa tcgcccacgg gataaacgca ctgatgatca gcgccagcgg caccagaatc
     4921 actttattga tcagcgatcc tttcgccacg ccccagacca cgggaagttc ccggttggcg
     4981 cgcacgcctg caacttgttg cgcattgagc gataagtcat cccctaatac accggcggtt
     5041 ttcttcgccg ccagtttgcc catcacggag atatcgtcca gcagtgtggc gatatcatcg
     5101 agcaacgtca gtaaactact cccggccaaa agcactccct ctcattttta ttgttgaagg
     5161 gtgtaagtat gaagcaaaaa cggcagctct gatacttgct tcacaggtca acatttattt
     5221 aacaatcaag tgcaattata tctctcgcca gcagaatgat tttcacgttt actatgaacc
     5281 acctttttat ttcagccgtg agggattatg cgtttccgcc agttgttacc gctttttggc
     5341 gcgctgtttg cgttgtatat catttggggc tcaacctatt ttgtcattcg gattggcgtg
     5401 gaaagctggc ctccgttaat gatggcgggc gttcgattcc tggcagccgg tattttattg
     5461 ctggcatttt tgctactgcg cggacacaaa cttcccccgc tacgtccgct gctcaatgcc
     5521 gcgctgattg gcctgttatt gctggctgtc ggtaatggca tggtgacggt tgccgaacat
     5581 caaaatgttc cttccggcat cgccgccgtt gtggttgcaa ccgtgcccct ctttaccctg
     5641 tgcttcagcc gcctgtttgg cattaaaacg cgcaaactgg aatgggtggg tatcgccatt
     5701 gggcttgccg gaatcatcat gctcaatagc ggtggaaatt taagcggcaa tccgtggggc
     5761 gcgattctga ttttaatcgg ctcgattagc tgggcgtttg gctcggttta tggctcgcgc
     5821 attaccttac ctgtagggat gatggcgggt gcgattgaga tgctggcggc tggcgtggtg
     5881 ttaatgattg cgtcgatgat tgcgggtgaa aaactgacgg cgctcccttc cctttcaggc
     5941 ttccttgcgg tcggctatct ggcgctgttt ggttcgatta tcgccattaa cgcttatatg
     6001 tatttaatcc gcaacgtcag tccggctctc gccaccagct acgcctacgt taacccagtg
     6061 gtcgcggtct tgctgggtac ggggctgggt ggcgaaacgc tgtcgaagat tgaatggctg
     6121 gcgctcggcg taattgtttt cgcggtggta ctggtcacgt tgggaaaata tctcttcccg
     6181 gccaaacccg ttgtcgcgcc agttattccg gatgcatcaa gcgagtaaat gaatcccctg
     6241 cgtgtcgatc tgcgcgctgg caccttcgcc gcagatccac tcttccagac gctcggtaag
     6301 cgccgcatcc gtcagcttct cgcgcccgcg taacgcacat tcccagacaa tcaatacgcg
     6361 ccaaccgagt tcctgcaaat ggctgatatc acgacgatcg cgctcgacat ttttacctat
     6421 cttctccagc caaaaatctg ttcgcgtggc ggggacttta aacaggtagc aatggtgatg
     6481 gtgccagaag caaccgtggg caaatatcac acagcgaaat tcatcaagga caaaatccgg
     6541 gcgtccgggc agactggcgt cctgaacgcg aaacgccagg ccctgcccag ttaacaaact
     6601 ggcgaggcgc ttctcaatcg ccgtatcacg cgtggcaatc gcgcgcatat ttttgctgcg
     6661 ggtggcctta tcgtgaacgt cgaccatgtt gtgcctcttg ctgacgcaac gccaccgcct
     6721 gtttgatttt tggctcaagc agttttgcca ccgcggcaaa gaccggcacg accaccgagt
     6781 taccgaactg gcgataagcc tgagtgtccg aaaccggaat gcggaatttc gcttctcccg
     6841 gcgcttcaaa acccattaag cgcgcacatt cccgaggcgt taaccgacgt gggcgatgtt
     6901 gctgattcag cggatcgtca aagtctttct cacccgttgc catatcccag ccgcgatcga
     6961 ttaaaatttc cgcgccatct ttgtaataac gcgcagacag cgtacgcgtg acgctttgcg
     7021 gattgttcgg ataaaccatt ccataaccga agccgttacc gcgcgcctga tgttttttcg
     7081 catatcgata gaggtacttc cacagtaccg gcgtcaggat atatttcgcc tcgaccatcg
     7141 ggtccagcag ctgcgccagc gtcactcgct gtgcagggaa acatttgctg atatcacgca
     7201 gagtaaaatc ggctttcaga ttcagatcgc gacgaaaacc caccagcacg atgcgttcac
     7261 ggtgctgcgg cagaaaatgt ttgccgtcga tgattttcgg atcgtccggc ccgttatctt
     7321 ctgcatcagc cacgtcatag cccagttcgt ccagcgtctg catgatgatg cggaacgttt
     7381 taccctggtc gtgacttttc aggtttttga cgttttcgag cacaaacatc gccggacgac
     7441 gcgcgtcgat aatgcgcacc acatcaaaga acagcgtgcc ctgggtatcg caggcaaaac
     7501 cgtgcgcccg cccgagcgag ttctttttcg atacgccagc cagcgaaaat ggctgacaag
     7561 ggaaaccggc cagtaaaaca tcgtgttcag gaatgtgttg acgaatatgt tccgccgccg
     7621 cctcatcact cacgccttct tgatggctga gggtgatgtc gcggatatcc tcattaaaat
     7681 gatgcgtcgc cgggtcgcaa taatggttgg ctttataagt gcgtaccgca tgtttgttcc
     7741 attcgctggt aaacacgcac tgtccaccaa tcgattcaaa accgcgacgg atgccgccaa
     7801 ttccggcaaa tagatcgata aagcgaaacg cataatgcgg atgatgtgcc ggtggtttgg
     7861 gtaacaacgt ttgcagatgg gcgaactctt tctcacttaa acggtgccat gccgagtcat
     7921 tcgccagcgc acgttttaaa attgccgcgc tccagtgatt ctcacccaca ccattaagct
     7981 gcgccaccag cgttttaaca tcataaattt gcagcagttt ctccagcatt gcctgtgcgg
     8041 cattcccggt gctgtatgaa tcggttactg atatattttc ctgcatagat ttaaccggcc
     8101 atctgagatg gccgacaggt taacataatt actgccgttt agggccacgc gctcagccag
     8161 caggtgaaaa cgcatctatc accttgtgat cgataccttc attctccccc gccagttcgg
     8221 caccgagctt cgccataaac tcgactaaaa attgcgcgtt gtgctgtgcc agctgcttgc
     8281 ctcttgcggt ttgcatggtt tgcggtaatt tcagtaactt tgtctggaaa tgatccagcg
     8341 cgtagcgttt atcatcaagc gggcgatgct gtgcgaacgg atcttcgcca tcaaacagcg
     8401 ccaagcccaa tgcccctgaa acggcaaata cgcgcgccag gccaatcgcc cccagggctt
     8461 ccagccgatc cgcatcctgg acgattttag cttccgttgt taagggggcg atttgcgcgc
     8521 tgaaactgtg agcggcaatg gcatgacaaa cggcctcaat tttctccgcc ggaaattgca
     8581 cgaactcttc ggtaatgctg ccaacttact gatttagtgt atgatggtga ttttaaggtg
     8641 cttgcgtggc ttccatttcc atcagatgtc cttcctgctc cgctactgaa ggcgtggtgc
     8701 gtaacggcaa aagcactgcc ggacatcagc gctatctctg ctctcattgc cgtaaaacat
     8761 ggcaactaca gttcacttac accgcctctc agcccggtaa gcaccagaaa atcattgata
     8821 tggccatgaa tggcgtcgga tgtcgcgcca gtgcacgcat tatgg
//
LOCUS       DAZKHF020000184         8788 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2201S31241
            SAMN43267800-rid25106413.denovo.185, whole genome shotgun sequence.
ACCESSION   DAZKHF020000184 DAZKHF020000000
VERSION     DAZKHF020000184.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267800
            Sequence Read Archive: SRR30307263
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 8788)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8788)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 8788)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350581.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350581.2
            Genome Coverage       :: 151x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:21:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,652
            CDSs (total)                      :: 5,557
            Genes (coding)                    :: 5,320
            CDSs (with protein)               :: 5,320
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 237
            CDSs (without protein)            :: 237
            Pseudo Genes (ambiguous residues) :: 0 of 237
            Pseudo Genes (frameshifted)       :: 102 of 237
            Pseudo Genes (incomplete)         :: 130 of 237
            Pseudo Genes (internal stop)      :: 52 of 237
            Pseudo Genes (multiple problems)  :: 41 of 237
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8788
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267800-rid25106413.denovo.185"
                     /serotype="O 26:H11"
                     /isolate="2201S31241"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-01"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            complement(8..634)
                     /gene="upp"
                     /locus_tag="ACHOSC_RS17470"
                     /old_locus_tag="pgaptmp_003494"
     CDS             complement(8..634)
                     /gene="upp"
                     /locus_tag="ACHOSC_RS17470"
                     /old_locus_tag="pgaptmp_003494"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001295473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004845 - uracil phosphoribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uracil phosphoribosyltransferase"
                     /protein_id="HGU4549374.1"
                     /translation="MKIVEVKHPLVKHKLGLMREQDISTKRFRELASEVGSLLTYEAT
                     ADLETEKVTIEGWNGPVEIDQIKGKKITVVPILRAGLGMMDGVLENVPSARISVVGMY
                     RNEETLEPVPYFQKLVSNIDERMALIVDPMLATGGSVIATIDLLKKAGCSSIKVLVLV
                     AAPEGIAALEKAHPDVELYTASIDQGLNEHGYIIPGLGDAGDKIFGTK"
     gene            959..1996
                     /gene="purM"
                     /locus_tag="ACHOSC_RS17475"
                     /old_locus_tag="pgaptmp_003495"
     CDS             959..1996
                     /gene="purM"
                     /locus_tag="ACHOSC_RS17475"
                     /old_locus_tag="pgaptmp_003495"
                     /EC_number="6.3.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001656828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004641 -
                     phosphoribosylformylglycinamidine cyclo-ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine cyclo-ligase"
                     /protein_id="HGU4549375.1"
                     /translation="MTDKTSLSYKDAGVDIDAGNALVGRIKGVVKKTRRPEVMGGLGG
                     FGALCALPQKYREPVLVSGTDGVGTKLRLAMDLKRHDTIGIDLVAMCVNDLVVQGAEP
                     LFFLDYYATGKLDVDTASAVISGIAEGCLQSGCSLVGGETAEMPGMYHGEDYDVAGFC
                     VGVVEKSEIIDGSKVSDGDVLIALGSSGPHSNGYSLVRKILEVSGCDPQTTQLDGKPL
                     ADYLLAPTRIYVKSVLELIEKVDVHAIAHLTGGGFWENIPRVLPDNTQAVIDESSWQW
                     PEVFNWLQTAGNVERHEMYRTFNCGVGMIIALPAPEVDKALALLNANGENAWKIGIIK
                     ASDSEQRVVIE"
     gene            1996..2634
                     /gene="purN"
                     /locus_tag="ACHOSC_RS17480"
                     /old_locus_tag="pgaptmp_003496"
     CDS             1996..2634
                     /gene="purN"
                     /locus_tag="ACHOSC_RS17480"
                     /old_locus_tag="pgaptmp_003496"
                     /EC_number="2.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004644 - phosphoribosylglycinamide
                     formyltransferase activity [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylglycinamide formyltransferase"
                     /protein_id="HGU4549376.1"
                     /translation="MNIVVLISGNGSNLQAIIDACKTNKIKGTVRAVFSNKADAFGLE
                     RARQAGIATHTLIASAFDSREAYDRELIHEIDMYAPDVVVLAGFMRILSPAFVSHYAG
                     RLLNIHPSLLPKYPGLHTHRQALENGDEEHGTSVHFVTDELDGGPVILQAKVPVFAGD
                     TEDDITARVQTQEHAIYPLVISWFADGRLKMHENAAWLDGQRLPPQGYAADE"
     gene            2805..4871
                     /gene="ppk1"
                     /locus_tag="ACHOSC_RS17485"
                     /old_locus_tag="pgaptmp_003497"
     CDS             2805..4871
                     /gene="ppk1"
                     /locus_tag="ACHOSC_RS17485"
                     /old_locus_tag="pgaptmp_003497"
                     /EC_number="2.7.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009358 - polyphosphate kinase complex
                     [Evidence IEA];
                     GO_function: GO:0008976 - polyphosphate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006799 - polyphosphate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyphosphate kinase 1"
                     /protein_id="HGU4549377.1"
                     /translation="MGQEKLYIEKELSWLSFNERVLQEAADKSNPLIERMRFLGIYSN
                     NLDEFYKVRFAELKRRIIISEEQGSNSHSRHLLGKIQSRVLKADQEFDGLYNELLLEM
                     ARNQIFLINERQLSVNQQNWLRHYFKQYLRQHITPILINPDTDLVQFLKDDYTYLAVE
                     IIRGDTIRYALLEIPSDKVPRFVNLPPEAPRRRKPMILLDNILRYCLDDIFKGFFDYD
                     ALNAYSMKMTRDAEYDLVHEMEASLMELMSSSLKQRLTAEPVRFVYQRDMPNALVEVL
                     REKLTISRYDSIVPGGRYHNFKDFINFPNVGKANLVNKPLPRLRHIWFDKAQFRNGFD
                     AIRERDVLLYYPYHTFEHVLELLRQASFDPSVLAIKINIYRVAKDSRIIDSMIHAAHN
                     GKKVTVVVELQARFDEEANIHWAKRLTEAGVHVIFSAPGLKIHAKLFLISRKENGEVV
                     RYAHIGTGNFNEKTARLYTDYSLLTADARITNEVRRVFNFIENPYRPVTFDYLMVSPQ
                     NSRRLLYEMVDREIANVQQGLPSGITLKLNNLVDKGLVDRLYAASSSGVPVNLLVRGM
                     CSLIPNLEGISDNIRAISIVDRYLEHDRVYIFENGGDKKVYLSSADWMTRNIDYRIEV
                     ATPLLDPRLKQRVLDIIDILFSDTVKARYIDKELSNRYVPRGNRRKVRAQLAIYDYIK
                     SLEQPE"
     gene            4876..6417
                     /gene="ppx"
                     /locus_tag="ACHOSC_RS17490"
                     /old_locus_tag="pgaptmp_003498"
     CDS             4876..6417
                     /gene="ppx"
                     /locus_tag="ACHOSC_RS17490"
                     /old_locus_tag="pgaptmp_003498"
                     /EC_number="3.6.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004309 - exopolyphosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006798 - polyphosphate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exopolyphosphatase"
                     /protein_id="HGU4549378.1"
                     /translation="MPIHDKSPRPQEFAAVDLGSNSFHMVIARVVDGAMQIIGRLKQR
                     VHLADGLGPDNMLSEEAMTRGLNCLSLFAERLQGFSPASVCIVGTHTLRQALNATDFL
                     KRAEKVIPYPIEIISGNEEARLIFMGVEHTQPEKGRKLVIDIGGGSTELVIGENFEPI
                     LVESRRMGCVSFAQLYFPGGVINKENFQRARMAAAQKLETLTWQFRIQGWNVAMGASG
                     TIKAAHEVLMEMGEKDGIITPERLEKLVKEVLRHRNFASLSLPGLSEERKTVFVPGLA
                     ILCGVFDALAIRELRLSDGTLREGVLYEMEGRFRHQDVRSRTASSLANQYHIDSEQAR
                     RVLDTTMQMYEQWREQQPKLAHPQLEALLRWAAMLHEVGLNINHSGLHRHSAYILQNS
                     DLPGFNQEQQLMMATLVRYHRKAIKLDDLPRFTLFKKKQFLPLIQLLRLGVLLNNQRQ
                     ATTTPPTLTLITDDSHWTLRFPHDWFSQNALVLLDLEKEQEYWEGVAGWRLKIEEEST
                     PEIAA"
     gene            complement(6456..8699)
                     /gene="pdeF"
                     /locus_tag="ACHOSC_RS17495"
                     /old_locus_tag="pgaptmp_003499"
     CDS             complement(6456..8699)
                     /gene="pdeF"
                     /locus_tag="ACHOSC_RS17495"
                     /old_locus_tag="pgaptmp_003499"
                     /EC_number="3.1.4.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic-guanylate-specific phosphodiesterase
                     PdeF"
                     /protein_id="HGU4549379.1"
                     /translation="MKLNATYIKIRDKWWGLPLFLPSLILPIFAHINTFAHISSGEVF
                     LFYLPLALMISMMMFFSWAALPGITLGIFVRKYAELGFYETLSLTANFIIIIILCWGG
                     YRVFTPRRNNVSHGDTRLISQRIFWQIVFPATLFLILFQFAAFVGLLASRENLVGVMP
                     FNLGTLINYQALLVGNLIGVPLCYFIIRVVRNPFYLRSYYSQLKQQVDAKVTKKEFAL
                     WLLALGALLLLLCMPLNEKSTIFSTNYTLSLLLPLMMWGAMRYGYKLISLLWAVVLMI
                     SIHSYQNYIPIYPGYTTQLTITSSSYLVFSFIVNYMAVLATRQRAVVRRIQRLAYVDP
                     VVHLPNVRALNRALRDAPWSALCYLRIPGMEMLVKNYGIMLRIQYKQKLSHWLSPLLE
                     PGEDVYQLSGNDLALRLNTESHQERITALDSHLKQFRFFWDGMPMQPQIGVSYCYVRS
                     PVNHIYLLLGELNTVAELSIVTNAPENMQRRGAMYLQRELKDKVAMMNRLQQALEHNH
                     FFLMAQPITGMRGDVYHEILLRMKGENDELIGPDSFLPVAHEFGLSSSIDMWVIEHTL
                     QFMAENRAKMPAHRFAINLSPTSVCQARFPVEVSQLLDKYQIEAWQLIFEVTESNALT
                     NVKQAQITLQHLQELGCQIAIDDFGTGYASYARLKNVNADLLKIDGSFIRNIVSNSLD
                     YQIVASICHLARMKKMRVVAEYVENEEIREAVLSLGIDYMQGYLIGKPQPLIDTLNEI
                     EPIRESA"
BASE COUNT         2271 a         2165 c         2175 g         2177 t
ORIGIN      
        1 ttattcttta tttcgtacca aagattttgt caccggcatc gccgaggccc ggaataatgt
       61 atccgtgctc gttcagtccc tgatcaatcg atgcggtata cagttcgacg tccgggtgcg
      121 ctttttccag cgcagcgata ccttctggcg cagctaccag caccagaact ttgatgctgc
      181 tgcagcccgc ttttttcagc aggtcgatgg tcgcgataac ggaaccaccg gttgccagca
      241 ttgggtcaac gatcagcgcc atacgctcat cgatgttaga aaccagtttc tggaagtacg
      301 gtaccggctc cagcgtttct tcattacggt acataccgac aacgctgatg cgcgcgctcg
      361 gaacattttc cagcacaccg tccatcatcc ccagaccggc acgcagaatt ggcacaacgg
      421 taattttctt acctttgatc tggtcgattt ctaccgggcc gttccagcct tcgatagtta
      481 ctttttccgt ttcgaggtcg gcggtcgctt cgtaagtcag caggctaccc acttcggaag
      541 cgagttcgcg aaagcgcttg gtgctgatat cttgctcacg catcagtccc agcttgtgtt
      601 tgacgagtgg gtgtttgact tccacgatct tcatactctt tctcctttga ggggcagcca
      661 caaaaaaaat cgacggatta tacctccttt cttcaaggcg gcaatattct tttcgttgac
      721 tttagtcaaa atgataacgg cttgagataa agttatttta tattcaaatg gttatgaaag
      781 aagattattc catccgaaaa ctaaccttta ccctggtaca agtcttcttt cgccgcgcgc
      841 ctgggcaaaa gacgtgcaaa aaggttgtgt aaagcagtct cgcaaacgtt tgctttccct
      901 gttagaattg cgccgaattt tatttttcta ccgcaagtaa cgcgtgggga cccaagcagt
      961 gaccgataaa acctctctta gctacaaaga tgccggtgtt gatattgacg cgggtaatgc
     1021 tctggttgga agaatcaaag gcgtagtgaa gaaaacgcgt cgtccggaag tgatgggcgg
     1081 tctgggcggc ttcggtgcgc tgtgtgcatt gccgcaaaaa tatcgtgaac ccgtgctggt
     1141 ttccggcact gacggcgtag gtaccaagct gcgtctggca atggacttaa aacgtcacga
     1201 caccattggt attgatctgg tcgccatgtg cgttaatgac ctggtggtgc aaggtgcaga
     1261 gccgctgttt ttcctcgact attacgcaac cggaaaactg gatgttgata ccgcttcagc
     1321 ggtgatcagc ggtatcgcgg aaggatgtct gcaatcgggc tgttcactgg tgggtggcga
     1381 aacggcagaa atgccgggga tgtatcacgg tgaggattac gatgtcgcgg gtttctgcgt
     1441 tggcgtggta gaaaaatcag aaatcatcga cggctctaaa gtcagcgacg gcgatgtgct
     1501 gattgcactc ggttccagcg gtccgcactc gaacggctat tcgctggtgc gcaaaattct
     1561 tgaagtcagc ggttgtgatc cgcaaaccac ccaacttgat ggtaagccat tagccgatta
     1621 tctgctggca ccgacccgca tttacgtgaa gtcagtgctg gagttgattg aaaaggtcga
     1681 tgtgcatgcc attgcgcacc tgaccggcgg cggcttctgg gaaaacattc cacgcgtatt
     1741 gccagataat actcaggcag tgattgatga atcttcctgg cagtggccgg aagtgttcaa
     1801 ctggctgcaa acggcaggta acgttgagcg ccatgaaatg tatcgcacct tcaactgcgg
     1861 cgtcgggatg attattgccc tgcctgctcc ggaagtggac aaagccctcg ccctgctcaa
     1921 tgccaacggt gaaaacgcgt ggaaaatcgg tatcatcaaa gcctctgatt ccgaacaacg
     1981 cgtggttatc gaataatgaa tattgtggtg cttatttccg gtaacggaag taatttacag
     2041 gcaattattg acgcctgtaa aaccaacaaa attaaaggca ccgtacgggc agttttcagc
     2101 aataaggccg acgcgttcgg ccttgaacgc gcccgccagg cgggtattgc aacgcatacg
     2161 ctcatcgcca gcgcgtttga cagtcgtgaa gcctatgacc gggagttgat tcatgaaatc
     2221 gacatgtacg cacccgatgt ggtcgtgctg gctggtttta tgcgcattct cagcccggcg
     2281 tttgtctccc actatgccgg gcgtttgctg aacattcacc cttctctgct gccgaaatat
     2341 cccggattac acacccatcg tcaagcgctg gaaaatggtg atgaagagca cggtacatcg
     2401 gtgcatttcg tcaccgatga actggacggt ggcccggtta ttttacaggc gaaagtcccg
     2461 gtatttgctg gtgatacgga agatgacatc accgcccgcg tgcaaaccca ggaacacgct
     2521 atttatccac tggtgattag ctggtttgcc gatggtcgtc tgaaaatgca cgaaaacgcc
     2581 gcgtggctgg atggtcaacg tctgccgccg cagggctacg ctgccgacga gtaatgcccc
     2641 gtagttaaag cgccagctct gccgctggcg tttttcaatt cacctgtaaa tcgcaagctc
     2701 cagcagtttt tttccccctt ttctggcata gttggacatc tgccaatatt gctcgccata
     2761 atatccaggc agtgtcccgt gaataaaacg gagtaaaagt ggtaatgggt caggaaaagc
     2821 tatacatcga aaaagagctc agttggttat cgttcaatga acgcgtgctt caggaagcgg
     2881 cggacaaatc taacccgctg attgaaagga tgcgtttcct ggggatctat tccaataacc
     2941 ttgatgagtt ctataaagtc cgcttcgctg aactgaagcg acgcatcatt attagcgaag
     3001 aacaaggctc caactctcat tcccgccatt tactgggcaa aattcagtcc cgggtgctga
     3061 aagccgatca ggaattcgac ggcctctaca acgagctatt gctggagatg gcgcgcaacc
     3121 agatcttcct gattaatgaa cgccagctct ccgtcaatca acaaaactgg cttcgtcatt
     3181 attttaagca gtatctgcgt cagcacatta cgccgatttt aatcaatcct gacactgact
     3241 tagtgcagtt cctgaaggat gattacacct atctggcggt ggaaattatc cgtggtgata
     3301 ccatccgtta cgcgctgctg gagatcccat cagataaagt gccgcgcttt gtgaatttac
     3361 cgccagaagc gccgcgtcga cgcaagccga tgattcttct ggataacatt ctgcgttact
     3421 gccttgatga tattttcaaa ggcttctttg attatgacgc gctgaatgcc tattcaatga
     3481 agatgacacg cgatgccgaa tacgatttag tgcatgagat ggaagccagc ctgatggagt
     3541 tgatgtcttc cagtctcaag cagcgtttaa ctgctgagcc ggtgcgtttt gtttatcagc
     3601 gcgatatgcc caatgcgctg gttgaagtgt tacgcgaaaa actgactatt tcccgctacg
     3661 actccatcgt ccccggcggt cgttatcata attttaaaga ctttattaat ttccccaatg
     3721 tcggcaaagc caatctggtg aacaaaccac tgccgcgttt acgccacatt tggtttgata
     3781 aagcccagtt ccgcaatggt tttgatgcca ttcgcgaacg cgatgtgttg ctctattatc
     3841 cttatcacac ctttgagcat gtgctggaac tgctgcgtca ggcttcgttc gacccgagcg
     3901 tactggcgat taaaattaac atttaccgcg tggcgaaaga ttcacgcatc atcgactcga
     3961 tgatccacgc cgcacataac ggtaagaaag tcactgtggt ggttgagtta caggcgcgtt
     4021 tcgacgaaga agccaacatt cactgggcga agcgcctgac cgaagcaggc gtgcacgtta
     4081 tcttctctgc gccggggctg aaaattcacg ccaaactgtt cctgatttca cgtaaagaaa
     4141 acggtgaagt ggtccgttac gcacacatcg ggaccgggaa ctttaacgaa aaaaccgcgc
     4201 gtctttatac tgactattcg ttgctgaccg cagatgcgcg tatcaccaac gaagtacggc
     4261 gggtatttaa ctttattgaa aacccatacc gcccagtgac atttgattat ttaatggtgt
     4321 caccgcaaaa ctctcgccgt ctgttatatg aaatggtaga ccgcgaaatc gccaacgtgc
     4381 agcaagggct gcccagtggt atcaccctga agctaaataa ccttgtcgat aaaggcctgg
     4441 ttgatcgtct gtatgcggcc tccagctccg gcgtaccggt taatctgctg gttcgcggaa
     4501 tgtgttcgct gatccccaat ctggaaggca ttagcgacaa cattcgtgcc atcagtattg
     4561 ttgaccgtta ccttgaacat gaccgggttt atatttttga aaatggcggc gataaaaagg
     4621 tctacctttc ttccgccgac tggatgacgc gcaatattga ttatcgtatt gaagtggcga
     4681 caccgctgct cgatccgcgc ctgaagcagc gggtgctgga catcatcgac atattgttca
     4741 gcgatacggt caaagcacgt tatatcgata aagaactcag taatcgctac gttccccgcg
     4801 gcaatcgccg caaagtacgg gcgcagttgg cgatttatga ctacatcaaa tcactcgaac
     4861 aacctgaata accctatgcc aatacacgat aaatcccctc gtccgcagga gtttgctgcg
     4921 gtcgatcttg gttcaaacag ttttcacatg gtcatagccc gtgtggtaga tggtgccatg
     4981 cagattattg gccgcctgaa acagcgggtg catctggcgg acggcctggg gccagataat
     5041 atgttgagtg aagaggcaat gacgcgcggt ttgaactgtc tgtcgctgtt tgccgaacgg
     5101 ctacaagggt tttctcctgc cagcgtctgt atagttggca cccatacact gcgtcaggcg
     5161 ctgaacgcca ctgactttct gaaacgcgcg gaaaaggtca ttccctaccc gattgaaatt
     5221 atttccggta atgaagaagc ccgtctgatt tttatgggcg tggaacatac ccaaccggaa
     5281 aaaggtcgca aactggttat tgatattggc ggcggatcta cggaactggt gattggtgaa
     5341 aatttcgaac ctattctcgt tgaaagccgc cggatgggtt gtgtcagctt tgcccagctt
     5401 tatttccctg gcggggtcat caataaagag aattttcagc gcgctcgcat ggcggcagca
     5461 caaaaactgg aaactttaac ctggcaattc cgtattcagg gctggaacgt ggcaatgggc
     5521 gcttccggta ccataaaagc cgcccatgaa gtattaatgg aaatgggcga gaaagacggg
     5581 ataattaccc cggaacgtct ggaaaaactg gtaaaagaag ttttacgtca ccgtaatttc
     5641 gcatcgctga gtttaccggg tctttccgaa gagcggaaaa cagtcttcgt tcctggactg
     5701 gcgattttat gtggtgtgtt tgatgcttta gccatccgtg aactgcgcct ttctgacggg
     5761 acgttacgcg aaggcgtact gtatgaaatg gaaggacgtt tccgtcatca ggatgtgcgt
     5821 agtcgcaccg ccagcagcct cgccaaccag tatcacatcg acagcgaaca ggcccgacga
     5881 gtgctggata ccactatgca aatgtacgaa cagtggcggg aacagcaacc gaagctggcg
     5941 catccgcaac tggaggcgct actgcgatgg gccgccatgc tgcatgaggt cgggttgaat
     6001 atcaaccaca gcggtttgca tcgccactcc gcttatattc tgcaaaacag tgacttgccg
     6061 ggttttaatc aggaacagca gctgatgatg gcgacactgg tgcgctatca ccgtaaagcg
     6121 attaagctcg acgatctgcc gcgctttacc ttgtttaaga agaaacagtt cctgccactg
     6181 atacagctat tgcgccttgg cgtattacta aacaatcaac gtcaggcaac caccacaccg
     6241 ccaacattga cactgattac tgatgacagt cactggacac tgcgtttccc gcatgactgg
     6301 tttagtcaga atgcgctggt actgcttgat ctggaaaagg agcaagaata ctgggaaggc
     6361 gtggctggct ggcggttgaa aattgaagaa gaaagtacac ctgaaatcgc cgcttaatgc
     6421 tttgcgggcc gacgagaaat gtcggcccgc attattcagg cactttcgcg aatgggttcg
     6481 atttcattca gcgtatcaat taacggttgc ggcttaccaa taagataacc ctgcatataa
     6541 tcgatcccca aagagagcac cgcctcgcgg atctcttcgt tttcaacgta ctctgccact
     6601 acccgcattt tcttcattcg cgccaggtgg caaatcgatg ccactatctg ataatccaga
     6661 ctatttgaca caatattgcg gataaaactg ccgtcaattt taagcagatc ggcattcaca
     6721 tttttaagcc gcgcatagct ggcgtagccg gtgccgaaat catcaatcgc aatctggcag
     6781 cctaattcct gaagatgctg caaggtgatt tgcgcctgct taacattggt cagagcatta
     6841 ctttcggtga cttcaaaaat aagttgccac gcttcaatct gatatttatc cagcaactga
     6901 ctgacttcaa caggaaaacg agcctgacat accgaggttg gagacagatt aatagcaaaa
     6961 cggtgagcgg gcatcttcgc tctgttttca gccataaatt gcagcgtatg ctcaatgacc
     7021 cacatgtcga tactcgacga taaaccaaat tcgtgcgcga ccggcaagaa gctatcgggg
     7081 ccgatcagtt catcattctc acctttcatg cgcagaagaa tttcatggta aacatcacca
     7141 cgcataccgg taatcggctg ggccatcagg aaaaaatggt tgtgttccag cgcctgctgt
     7201 agtcgattca tcatcgcgac tttatctttc aattcgcgtt gcaaatacat tgccccgcga
     7261 cgctgcatat tttccggggc gttggtcacg atggaaagtt cggcgaccgt atttagctct
     7321 cccagcagca ggtagatatg attcactggc gagcgcacat agcagtaact gacgccaatc
     7381 tgcggttgca tcggcatgcc atcccaaaag aaacgaaatt gcttgagatg gctatccagt
     7441 gcggtaatgc gctcctggtg cgattctgta ttcagccgca gcgcgagatc gttacccgaa
     7501 agctgataaa catcttcacc cggttccagc aagggtgaca gccagtgaga aagtttttgc
     7561 ttgtattgaa tccgcagcat gatgccatag ttcttaacca gcatttccat gccagggatg
     7621 cgtaaataac aaagcgcaga ccagggggca tcacgcaacg cgcgattcag ggcgcgaaca
     7681 tttggcagat gaaccaccgg gtccacatac gcaagccgct gaatgcgtct gactaccgct
     7741 cgctgacgag ttgccagtac agccatataa ttgacaataa aagagaatac cagataactg
     7801 gaggaggtta tggtcagctg cgtggtatag ccaggataaa tgggaatgta attttgatag
     7861 ctgtggatgc tgatcatcaa cacgaccgcc cagagcagcg aaatcagctt ataaccatag
     7921 cgcatcgctc cccacatcat caggggcagc aataatgaca aggtataatt ggtgctaaaa
     7981 attgtgcttt tttcatttaa cggcatgcat aacagcaaca ataaagcacc taatgccagt
     8041 agccagagcg cgaactcttt tttggtgact ttggcatcaa cctgctgttt taattgcgaa
     8101 taatagctac gtaaataaaa tggatttcgc actacccgaa tgatgaagta gcacagcggg
     8161 acaccgatca gattacccac cagtaaggcc tgataattga ttaaagtccc gaggttaaaa
     8221 ggcatgacac cgaccagatt ttctctgctc gccagtaatc ctacaaatgc agcaaactgg
     8281 aaaagtatca gaaacagcgt tgcaggaaac acaatctgcc agaatatacg ctgggaaatt
     8341 aaacgggtat caccatgtga aacgttgtta cgccggggag taaagaccct gtaaccgccc
     8401 caacagagaa tgataatgat aataaaatta gccgttaatg agagcgtttc gtaaaaaccc
     8461 agctctgcat atttgcggac aaaaatccct aaggtgatcc ctggcaatgc cgcccagctg
     8521 aaaaacatca tcatgctgat catcagtgcc aaaggcagat aaaagagaaa aacctcaccg
     8581 gaagaaatat gcgcgaaagt attaatgtgg gcgaaaatgg gcaagattaa agaaggcagg
     8641 aacagcggaa gcccccacca tttatcacgt atttttatat aagttgcatt cagtttcata
     8701 gatgctcagc agaatccccc acatcctgaa ggaggtgtat tcagacaggc atcccacctg
     8761 acttcgaatg atgattattc atcactat
//
LOCUS       DAZKHF020000185         8745 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2201S31241
            SAMN43267800-rid25106413.denovo.186, whole genome shotgun sequence.
ACCESSION   DAZKHF020000185 DAZKHF020000000
VERSION     DAZKHF020000185.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267800
            Sequence Read Archive: SRR30307263
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 8745)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8745)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 8745)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350581.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350581.2
            Genome Coverage       :: 151x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:21:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,652
            CDSs (total)                      :: 5,557
            Genes (coding)                    :: 5,320
            CDSs (with protein)               :: 5,320
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 237
            CDSs (without protein)            :: 237
            Pseudo Genes (ambiguous residues) :: 0 of 237
            Pseudo Genes (frameshifted)       :: 102 of 237
            Pseudo Genes (incomplete)         :: 130 of 237
            Pseudo Genes (internal stop)      :: 52 of 237
            Pseudo Genes (multiple problems)  :: 41 of 237
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8745
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267800-rid25106413.denovo.186"
                     /serotype="O 26:H11"
                     /isolate="2201S31241"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-01"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            353..904
                     /locus_tag="ACHOSC_RS17500"
                     /old_locus_tag="pgaptmp_003500"
     CDS             353..904
                     /locus_tag="ACHOSC_RS17500"
                     /old_locus_tag="pgaptmp_003500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001273868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000150 - DNA strand exchange activity
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HGU4549380.1"
                     /translation="MANIGYIRVSTVQQNTDRQLAGVALDKVFEDKASGKNTQRPQFE
                     AMMNYVREGDVLHVHSIDRLCRNTADLLATVEQLTERGVSVHFHKEDFKTGKNSPAGN
                     MMLTVLAAVAQMEREMMLERQREGIAAAKAAGRIAKRGNGKAIDRAGIAAALANGGSI
                     RSVAKDFSVSTQTVQRIKKEQEA"
     gene            1557..1877
                     /gene="yecR"
                     /locus_tag="ACHOSC_RS17505"
                     /old_locus_tag="pgaptmp_003501"
     CDS             1557..1877
                     /gene="yecR"
                     /locus_tag="ACHOSC_RS17505"
                     /old_locus_tag="pgaptmp_003501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014885115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YecR family lipoprotein"
                     /protein_id="HGU4549381.1"
                     /translation="MRKSLMLLLASTLIISGCATKKQLVPTGGSKSDGTVRMSYSYGM
                     FESPVVDPQQGMNAAKARCAAWGYSGAEPFGGFTSQCSQPSSSGCMQTLVTIEYQCTG
                     DIKK"
     gene            complement(1911..2168)
                     /locus_tag="ACHOSC_RS17510"
                     /old_locus_tag="pgaptmp_003502"
     CDS             complement(1911..2168)
                     /locus_tag="ACHOSC_RS17510"
                     /old_locus_tag="pgaptmp_003502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001570270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4549382.1"
                     /translation="MVSIVYNNLTIPLHEVDMTVSYKITYEHRDGDQIRMEEVLFHSE
                     HEPTKDEIHDAVRKDTVRFNSPSNGVTAVSGFKVISVVPVP"
     gene            complement(2615..2688)
                     /locus_tag="ACHOSC_RS17515"
                     /old_locus_tag="pgaptmp_003503"
     tRNA            complement(2615..2688)
                     /locus_tag="ACHOSC_RS17515"
                     /old_locus_tag="pgaptmp_003503"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2654..2656),aa:Gly,seq:ccc)
     gene            complement(2767..3522)
                     /gene="actS"
                     /locus_tag="ACHOSC_RS17520"
                     /old_locus_tag="pgaptmp_003504"
     CDS             complement(2767..3522)
                     /gene="actS"
                     /locus_tag="ACHOSC_RS17520"
                     /old_locus_tag="pgaptmp_003504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311765.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidase activator ActS"
                     /protein_id="HGU4549383.1"
                     /translation="MSAGRLNKKSLGIVMLLSVGLLLAGCSGSKSSDTGTYSGSVYTV
                     KRGDTLYRISRTTGTSVKELARLNGISPPYTIEVGQKLKLGGAKSSSSTRKSTAKSTT
                     KTASVTPSSAVPKSSWPPVGQRCWLWPTTGKVIMPYSTADGGNKGIDISAPRGTPIYA
                     AGAGKVVYVGNQLRGYGNLIMIKHSEDYITAYAHNDTMLVNNGQSVKAGQKIATMGST
                     DAASVRLHFQIRYRATAIDPLRYLPPQGSKPKC"
     gene            3937..6234
                     /gene="xdhA"
                     /locus_tag="ACHOSC_RS17525"
                     /old_locus_tag="pgaptmp_003505"
     CDS             3937..6234
                     /gene="xdhA"
                     /locus_tag="ACHOSC_RS17525"
                     /old_locus_tag="pgaptmp_003505"
                     /EC_number="1.17.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase molybdenum-binding
                     subunit XdhA"
                     /protein_id="HGU4549384.1"
                     /translation="MEAREATATGESCMRVDAIAKVTGRARYTDDYVMAGMCYAKYVR
                     SPIAHGYAVSINDEQARSLPGVLAIFTWEDVPDIPFATAGHAWTLDENKRDTADRALL
                     TRHVRHHGDAVAIVVARDELTAEKAAQLVSIEWQELPVITTPEAALAEDAAPIHNGGN
                     LLKQSTMSTGNVQQTIDAADYQVQGHYQTPVIQHCHMESVTSLAWMEDDSRITIVSST
                     QIPHIVRRVVGQALDIPWSCVRVIKPFVGGGFGNKQDVLEEPMAAFLTSKLGGIPVKV
                     SLSREECFLATRTRHAFTIDGQMGVNRDGTLKGYSLDVLSNTGAYASHGHSIASAGGN
                     KVAYLYPRCAYAYSSKTCYTNLPSAGAMRGYGAPQVVFAVESMLDDAATALGIDPVEI
                     RLRNAAREGDANPLTGKRIYSAGLPECLEKGRKIFEWEKRRAECQNQQGNLRRGVGVA
                     CFSYTSNTWPVGVEIAGARLLMNQDGTINVQSGATEIGQGADTVFSQMVAETVGVPVS
                     DVRVISTQDTDVTPFDPGAFASRQSYVAAPALRSAALLLKEKIIAHAAVMLHQSAMNL
                     TLIKGHIVLVERPEEPLMSLKDLAMDAFYHPERGGQLSAESSIKTTTNPPAFGCTFVD
                     LTVDIALCKVTINRILNVHDSGHILNPLLAEGQVHGGMGMGIGWALFEEMIIDAKSGV
                     VRNPNLLDYKMPTMPDLPQLESAFVEINEPQSAYGHKSLGEPPIIPVAAAIRNAVKMA
                     TGVAINTLPLTPKRLYEEFHLAGLI"
     gene            6245..7123
                     /gene="xdhB"
                     /locus_tag="ACHOSC_RS17530"
                     /old_locus_tag="pgaptmp_003506"
     CDS             6245..7123
                     /gene="xdhB"
                     /locus_tag="ACHOSC_RS17530"
                     /old_locus_tag="pgaptmp_003506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase FAD-binding subunit XdhB"
                     /protein_id="HGU4549385.1"
                     /translation="MFDFASYHRAATLADAINLLADNPQAKLLAGGTDVLIQLHHHND
                     RYRHIVDIHNLAELRGITLAEDGSLRIGSATTFTQLIEDPITQRHLPALCAAATSIAG
                     PQIRNVATYGGNICNGATSADSATPTLIYDAKLEIHSPRGVRFVPINGFHTGPGKVSL
                     EHDEILVAFHFPPQPKEHAGSAHFKYAMRDAMDISTIGCAAHCRLDNGNFSELRLAFG
                     VAAPTPIRCQHAEQTAQNAPLNLQTLEAISESVLQDVAPRSSWRASKEFRLHLIQTMT
                     KKVISEAVAAAGGKLQ"
     gene            7120..7599
                     /gene="xdhC"
                     /locus_tag="ACHOSC_RS17535"
                     /old_locus_tag="pgaptmp_003507"
     CDS             7120..7599
                     /gene="xdhC"
                     /locus_tag="ACHOSC_RS17535"
                     /old_locus_tag="pgaptmp_003507"
                     /EC_number="1.17.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase iron sulfur-binding
                     subunit XdhC"
                     /protein_id="HGU4549386.1"
                     /translation="MNHSETITIECTINGMPFQLHAAPGTPLSELLREQGLLSVKQGC
                     CVGECGACTVLVDGTAIDSCLYLAAWAEGKEIRTLEGEAKGGKLSHVQQAYAKSGAVQ
                     CGFCTPGLIMATTAMLAKPREKPLTITEIRRGLAGNLCRCTGYQMIVNTVLDCEKTK"
     gene            complement(7639..>8745)
                     /locus_tag="ACHOSC_RS17540"
                     /old_locus_tag="pgaptmp_003508"
     CDS             complement(7639..>8745)
                     /locus_tag="ACHOSC_RS17540"
                     /old_locus_tag="pgaptmp_003508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 interaction domain-containing protein"
                     /protein_id="HGU4549387.1"
                     /translation="VSWDDKSELIIGQLHNIQGRQLFLMAFHQSHTSFSVANAPDEPH
                     IEQLVGECRVMRQLKRLISRIAPSPSSVMVVGESGTGKEVVARAIHKLSGRRNKPFIA
                     INCAAIPEQLLESELFGYVKGAFTGASANGKTGLIQAANTGTLFLDEIGDMPLMLQAK
                     LLRAIEAREILPIGASSPIQVDIRIISATNQNLAQFIAEGKFREDLFYRLNVIPITLP
                     PLRERQEDIELLVHYFLHLHTRRLGSVYPGIAPDVVEILRKHRWPGNLRELSNLMEYL
                     VNVVPSGEVIDSTLLPPNLLNNGTTEQSDVTEVSEAHLSLDDAGGTALEEMEKQMIRE
                     ALSRHNSKKQVADELGIGIATLYRKIKKYELLNT"
BASE COUNT         2333 a         2145 c         2034 g         2233 t
ORIGIN      
        1 gagcaatgtt agctttcttc acttttacat tatcatcagc aaagccttat gtaatgcggc
       61 atatagcgca tcactgcaaa attgttgctc aataaacgtt ctatcattta ttgaacaagt
      121 ttcctttaat gcaacaaaaa attatttttt caaaataaac acactattta atagcttgct
      181 aattttacca aaatgtaact acatgattct catgcaagac gtgtagtgat aaagcaggct
      241 aaatcaccac actaaaaacc ttcgaatttc gtagtgaaaa aagtccttta cacacctaat
      301 caacacgatc aataatcact acacacaaat caacacagaa ggaataaaga aaatggctaa
      361 tattggatac attagggtat cgactgtgca acaaaacaca gaccgtcaat tagctggagt
      421 agctcttgat aaagtctttg aagataaagc gtcaggcaag aatacccagc gcccacagtt
      481 tgaggcaatg atgaactatg tgcgggaagg tgatgttttg catgttcaca gtattgatcg
      541 actttgccgt aatacagccg acctactggc aacggttgag caattaacag agcgcggcgt
      601 gtctgtgcat ttccataaag aagactttaa gacagggaag aactctcccg cagggaatat
      661 gatgctcaca gtgcttgcag cagtggcaca aatggaacgt gaaatgatgc ttgagcgcca
      721 gcgtgagggg atcgccgcag ctaaggccgc tggacgtata gcaaaacgtg gtaatggaaa
      781 agcaattgat cgagcgggaa ttgctgccgc tctcgcgaat ggtggatcaa ttcggagcgt
      841 agcaaaagat ttcagtgtca gcacacaaac ggtgcaacgt atcaagaaag agcaggaagc
      901 gtaatgcctc ctgatatttc agaattgtcg ctgcatggac ctgacggcgc tgatcttctc
      961 gttaagcgat gatcacgtat gcgccgattt cagagaaaaa gacagtaact gaactcacta
     1021 tgaaggctaa acgtatcttt acacttgatg gaaacgtcca aaaaacaacc taaccccaaa
     1081 acccaacaaa ttgcaaaata ttgtttttaa aagaaaatca aatctacatc aaatattggt
     1141 tgacgtagat tgaggtgaat agtagactat taggataaat taccagtatt gcacacaaca
     1201 aatttgcatg atttaatccc cctcacattc atttaggggg cgttatgcat ttatcttcaa
     1261 aacatcttag cactatccgc tttctcgtta ttgcttcatt cgttgtagct atcagttacc
     1321 gactttttgt tgcctccaat gacgaaatga atcctcttgc tattattgcc cattcaatag
     1381 cgacaatagt tagacggttt gaaaaaccgt aatacaataa acatccgaaa taatttcgaa
     1441 agcaactcat ttttactctg caaacaatca gaaaagactt aattttgcta agttaatgca
     1501 ttaatctaaa aacgctttaa atcaatacat aaccattatc tcttgggatt atttacatgc
     1561 gcaaaagttt aatgctgtta cttgcttcaa cattgattat ttctggatgt gcaacaaaaa
     1621 aacaactcgt tccaactggt ggaagcaaat cagacggcac agttaggatg agttattctt
     1681 acggtatgtt tgaaagtcct gttgtagatc cacaacaagg catgaatgca gccaaagcgc
     1741 gttgtgccgc ttggggatat tcaggagccg agccttttgg tggattcact tctcaatgtt
     1801 cacaaccttc ttcatctggt tgtatgcaaa cacttgtgac tatcgaatat caatgcactg
     1861 gcgatattaa gaagtaataa ttaggggctt ccagcccctt ctataaccat tcatggaaca
     1921 gggacaaccg aaatcacttt aaaccctgaa acagccgtta ctccattaga tggagaatta
     1981 aatctcacag tatcttttct tactgcatca tgaatttcat cttttgttgg ttcatgctct
     2041 gaatggaata aaacctcttc cattctaatt tgatcaccgt ctctatgctc ataagtaatc
     2101 ttgtagctaa cagtcatatc aacctcatgc aggggaattg tgagattatt atagacaata
     2161 gaaaccataa agccccgttg gtgatacagc aacaagcggg agcgtagcga cttcatcaga
     2221 ttgtcagaaa gatcacacct tatcaggata accgtgtacc cgttgggagc gtggctgaaa
     2281 cgctgataga gcttgcatat ggtttgcaag gaccaatatc accataaacc tgtttacgca
     2341 gccacaaggc cgcacatcgg gcttaaatgc gtgtgttcag tctgtcgatg acattacaat
     2401 gccaggatgg gctttatctt cacgttgtgc tatcgcggtg tgagaggtgc aactgccgcc
     2461 aacgttaggt tatactgtca tctcaccgtg taacttaacg tgacagttac taagggcgga
     2521 tcacaatggt agaactccta agcaaattaa ataggtaact gtttaagttg ctgacagaga
     2581 agactttttt agtcttctcc acctggggag attctggagc gggcgaaggg aatcgaaccc
     2641 tcgtatagag cttgggaagc tcttgttcta ccattgaact acgcccgctt cgagatgcgt
     2701 aaggcattat aaaccttacg ctctccttag caagtgccgc gctgctgact gctgattaat
     2761 tcgccatcag cattttggct tgctgccctg cggcggcaag taacgtagcg gatcaattgc
     2821 cgttgcacgg taacgaatct ggaaatgcag gcgaacagat gccgcatccg tgctccccat
     2881 agtggcgatt ttttgcccag ccttcacgct ttgcccatta tttaccagca tcgtgtcatt
     2941 atgggcgtaa gccgtaatgt aatcttcact gtgtttaatc atgatgagat taccgtagcc
     3001 acgcagctgg ttgcccacat acaccacctt tcctgcaccc gcggcgtaaa taggtgtacc
     3061 ccgtggagct gagatatcaa tccctttatt gccgccatct gctgtcgaat acggcatgat
     3121 aactttccct gtcgttggcc ataaccaaca acgttgccct actggcggcc aggaagattt
     3181 cggtaccgct gatgacggtg taaccgatgc ggttttggtc gttgatttgg cggttgattt
     3241 acgtgtacta ctgctacttt tcgccccacc cagttttagt ttctgaccaa cttcaatggt
     3301 gtaagggggg gaaatgccgt tcagtcgcgc cagttctttt acgctggttc ccgtggtgcg
     3361 cgaaatacga tatagcgtat ccccccgttt cacggtgtaa acggagccgg aatacgttcc
     3421 tgtatcggat gatttgctac ccgaacagcc cgccaaaagc agtccaaccg ataacaacat
     3481 cacgataccc agagattttt tattcaggcg tcccgcactc aaaatctttc ctcgcttaaa
     3541 aaacaagacg ccttatcata gcagccagcc tcttgatacc caatctcttg cttctggaaa
     3601 aacgtggtaa tagagaaaga ggttaatttc atttggcaaa gggaagtatc atcagcaaaa
     3661 tattcgctgt agaggtatcg aacttcctga ctttttaccc tatttcccat cctgatgcgt
     3721 aataatccca atttatcaca ttgattttat cgccactcat attgatacgt atcactgccc
     3781 ctattcagag tcctgtttcg tatcatatct gtgttttttt aacgtcatca cacttcctac
     3841 ctcccctaac gcttatcgtc gtttctggcg taaatcttgc ctgcttagac taaatctttg
     3901 ccataagaac caaatgttca aatcgagggg atttctatgg aagcgcggga agcaaccgct
     3961 acgggtgaat catgcatgcg cgtcgatgcc attgctaagg tcaccgggcg ggcacgatat
     4021 actgacgatt atgttatggc gggcatgtgt tatgcgaaat atgtacgtag ccctatcgca
     4081 catggttatg ccgtaagtat taatgatgaa caagccagaa gtttgccggg cgtactggcg
     4141 atttttacct gggaagatgt gcctgatatt ccatttgcta ctgctgggca tgcctggaca
     4201 cttgacgaaa acaagcgcga taccgccgat cgcgcactgc taactcgcca tgttcgtcat
     4261 catggtgacg ccgttgccat cgtcgtggcc cgcgatgaac tcacggcaga aaaagcggcg
     4321 caattggtca gcattgagtg gcaagaatta cccgttatca ccacgccaga agcggcgctg
     4381 gcagaagacg ctgcaccaat ccataacggt ggcaatttac tgaaacaaag cacgatgtcg
     4441 acgggtaatg tccaacaaac aatcgatgcc gccgactacc aggtacaggg gcactatcag
     4501 acccccgtta ttcaacattg tcacatggaa agcgtaacat cgctggcgtg gatggaggat
     4561 gactcgcgaa ttaccatcgt ttccagcacc cagatcccgc acattgttcg ccgcgtggtt
     4621 ggtcaggcgc tggatattcc ctggtcatgc gtacgagtca tcaaaccgtt tgtcggtggc
     4681 ggttttggta ataaacagga tgtactggaa gagccaatgg cggcattcct gaccagcaag
     4741 cttggcggca ttccggtgaa agtttccctt agccgtgaag agtgtttcct cgcaacccgt
     4801 acccgccacg cttttaccat tgacgggcaa atgggcgtga accgcgacgg aacattgaaa
     4861 ggttatagtc tggatgttct gtctaacacc ggcgcttatg catctcacgg gcactccatc
     4921 gcttctgcgg gggggaataa agtcgcttac ctttatcctc gttgtgccta cgcttacagt
     4981 tcaaagacct gctataccaa cctcccctcg gctggtgcga tgcgtggtta tggcgcgcca
     5041 caagtcgtat ttgccgttga gtctatgctt gatgacgccg cgacagcgtt aggtattgat
     5101 cctgttgaaa ttcgtttacg caacgccgcc cgcgaaggag atgctaatcc gctcacgggc
     5161 aaacgtattt acagcgcagg gttgccggag tgtcttgaaa aaggccggaa aatctttgaa
     5221 tgggaaaaac gccgtgcaga atgccagaac cagcaaggca atttgcgccg cggcgttggc
     5281 gtcgcctgtt ttagctacac ctctaacacc tggcctgtcg gcgtagaaat agcaggcgcg
     5341 cgccttctga tgaatcagga tggaaccatc aacgtgcaaa gcggcgcgac ggaaatcggt
     5401 cagggtgccg acaccgtctt ctcgcaaatg gtggcagaaa ccgtgggggt tccggtcagc
     5461 gacgttcgcg ttatttcaac tcaagatacc gacgttacgc cgttcgatcc cggcgcattt
     5521 gcctcacgcc agagctatgt tgccgcgcct gcgctgcgca gtgcggcact attattaaaa
     5581 gagaaaatca tcgctcacgc cgcagtcatg ctacatcagt cagcgatgaa tctgaccctg
     5641 ataaaaggcc atatcgtgct ggttgaacga ccggaagagc cgttaatgtc gttaaaagat
     5701 ttggcgatgg acgctttcta ccaccctgaa cgcggcgggc agctctctgc tgaaagctcc
     5761 atcaaaacca ccactaaccc accggcgttt ggctgtacct ttgttgatct gacggtcgat
     5821 attgcgctgt gcaaagtcac catcaaccgc atcctcaacg ttcatgattc agggcatatt
     5881 cttaatccac tgctggcaga aggtcaggta cacggcggaa tgggaatggg cattggctgg
     5941 gcgctatttg aagagatgat catcgatgct aaaagcggcg tggtccgtaa ccccaatctg
     6001 ctggattaca aaatgccgac catgccggat ctgccacaac tggaaagcgc gttcgtcgaa
     6061 atcaatgagc cgcaatccgc atacggacat aagtcactgg gtgagccacc aataattcct
     6121 gttgccgctg ctattcgtaa cgcggtgaag atggctaccg gtgttgcaat caatacactg
     6181 ccgctgacgc caaaacggtt atatgaagag ttccatctgg caggattgat ttgaggataa
     6241 catcatgttt gattttgctt cttaccatcg cgcagcaacc cttgccgatg ccatcaacct
     6301 gctggctgac aacccgcagg ccaaactgct cgccggtggc actgacgtac tgattcagct
     6361 ccaccatcac aatgaccgtt atcgccatat tgttgatatt cataatctgg cggagctgcg
     6421 gggaattacg ctggcggaag atggctcgct acgtatcggc tctgcaacga catttaccca
     6481 gctaatagaa gatcctataa ctcaacgtca tctcccggcg ttatgtgctg cggccacgtc
     6541 cattgctgga ccgcagatcc gtaacgtcgc tacctacggt ggaaatattt gcaacggtgc
     6601 caccagcgca gattctgcca cgccaacgct aatttatgac gcgaaactgg agatccactc
     6661 cccgcgcggt gttcgtttcg tcccgattaa tggctttcac accgggccgg gcaaagtgtc
     6721 tcttgagcat gacgaaatcc tcgtcgcctt tcattttccg ccacagccga aagaacacgc
     6781 gggcagcgcg cattttaaat atgccatgcg cgacgcaatg gatatttcaa cgattggctg
     6841 cgccgcacat tgccgactgg ataacggcaa tttcagcgaa ttacgcctgg catttggtgt
     6901 tgccgcgcca acgccgattc gctgccaaca tgccgaacag actgcacaaa atgcgccatt
     6961 aaacctgcaa acgctggaag ctatcagcga atctgtcctg caagatgtcg ccccgcgttc
     7021 ttcatggcgg gccagtaaag agtttcgtct gcatctcatc cagacgatga ccaaaaaagt
     7081 gattagcgaa gccgtcgccg cggcgggggg aaaattgcaa tgaatcacag cgaaacaatt
     7141 accatcgaat gcaccattaa cgggatgcct tttcagcttc acgccgcacc aggcacgccg
     7201 ctctcggaat tactccgcga acaaggactg ctaagtgtca aacaagggtg ctgcgtgggt
     7261 gaatgtggtg cctgtacggt gttggtcgac ggcacagcaa tagacagttg cttatacctt
     7321 gccgcctggg ctgaaggaaa agagatccgc acgctggaag gtgaagcgaa aggcggaaaa
     7381 ctttctcatg ttcagcaggc ttatgcgaaa tccggcgcag tgcagtgcgg gttttgtacg
     7441 cctggcctga ttatggctac cacggcaatg ctggcgaaac cacgcgagaa gccattaacc
     7501 attacggaaa ttcgtcgcgg actggcggga aatctttgtc gctgcacggg gtatcagatg
     7561 attgtaaata cagttctgga ttgcgagaaa acgaagtaaa aggatatccg gcctgaattc
     7621 aggccggatt cactgaggtt atgtgtttaa caactcatat ttcttaatct tgcgatagag
     7681 cgtagcaatg ccgatgccca gttcatcagc aacttgcttc ttgctgttat gacgtgaaag
     7741 cgcctcgcgg atcatttgct tttccatctc ctccagcgcc gtgccgcccg catcatcgag
     7801 tgacaggtgc gcctcactga cctctgttac atcactttgc tccgttgtgc cattattcag
     7861 cagatttggc ggcaatagcg tgctgtcgat aacttcacct gaaggaacta cgttaaccag
     7921 atattccatc aaattgctta actcgcgcag gtttccgggc caacgatgct tacgcaatat
     7981 ttcgacgaca tcgggagcaa tgccaggata aaccgatccc agacgacggg tatgcagatg
     8041 taaaaagtaa tgcaccaata gttcaatatc ttcctgacgt tcacgcagcg gtggcagagt
     8101 tatcgggata acattaagtc ggtagaagag atcttcgcgg aatttacctt cggcaatgaa
     8161 ctgggccaaa ttctgattag ttgcagaaat gatgcgaatg tcgacttgta ttgggctact
     8221 ggcaccaatc ggtagaattt cacgcgcctc aatagcgcgc agtaatttag cctgcaacat
     8281 taatggcata tcacctattt catcgagaaa cagcgtgccc gtattcgccg cctgaatcaa
     8341 ccctgtttta ccgttggcag aagcgccagt aaatgcgcct ttaacataac cgaacagttc
     8401 gctttccaga agctgctccg gaatcgcggc acagttgata gcaataaagg gtttattccg
     8461 tcttccgctc aacttatgga ttgctcgggc gacgacttct ttacccgtgc cgctttcacc
     8521 aaccaccata acgctggatg ggctgggtgc aatacggcta atgagtcgtt ttaattgccg
     8581 cataacacgg cactcgccaa ccaattgttc aatatgtggt tcatcaggtg catttgctac
     8641 agaaaaactg gtatgcgatt ggtgaaatgc cattaaaaat aattgtcggc cctgaatgtt
     8701 atgcaattga ccaatgatta attcactttt atcgtcccac gaaac
//
LOCUS       DAZKHF020000186         8622 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2201S31241
            SAMN43267800-rid25106413.denovo.187, whole genome shotgun sequence.
ACCESSION   DAZKHF020000186 DAZKHF020000000
VERSION     DAZKHF020000186.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267800
            Sequence Read Archive: SRR30307263
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 8622)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8622)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 8622)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350581.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350581.2
            Genome Coverage       :: 151x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:21:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,652
            CDSs (total)                      :: 5,557
            Genes (coding)                    :: 5,320
            CDSs (with protein)               :: 5,320
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 237
            CDSs (without protein)            :: 237
            Pseudo Genes (ambiguous residues) :: 0 of 237
            Pseudo Genes (frameshifted)       :: 102 of 237
            Pseudo Genes (incomplete)         :: 130 of 237
            Pseudo Genes (internal stop)      :: 52 of 237
            Pseudo Genes (multiple problems)  :: 41 of 237
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8622
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267800-rid25106413.denovo.187"
                     /serotype="O 26:H11"
                     /isolate="2201S31241"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-01"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            <1..1326
                     /gene="xanP"
                     /locus_tag="ACHOSC_RS17545"
                     /old_locus_tag="pgaptmp_003509"
     CDS             <1..1326
                     /gene="xanP"
                     /locus_tag="ACHOSC_RS17545"
                     /old_locus_tag="pgaptmp_003509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine/proton symporter XanP"
                     /protein_id="HGU4549388.1"
                     /translation="IYRLEDRPPLPQTLFAACQHLLAMFVAVITPALLICQALGLPAQ
                     DTQHIISMSLFASGVASIIQIKAWGPVGSGLLSIQGTSFNFVAPLIMGGTALKTGGAD
                     VPTMMAALFGTLMLASCTEMVISRVLHLARRIITPLVSGVVVMIIGLSLIQVGLTSIG
                     GGYAAMSDNTFGAPKNLLLAGVVLALIILLNRQRNPYLRVASLVIAMAAGYALAWFMG
                     MLPESNEPMTQELIMVPTPLYYGLGIEWSLLLPLMLVFMITSLETIGDITATSDVSEQ
                     PVSGPLYMKRLKGGVLANGLNSFVSAVFNTFPNSCFGQNNGVIQLTGVASRYVGFVVA
                     LMLIVLGLFPAVSGFVQHIPEPVLGGATLVMFGTIAASGVRIVSREPLNRRAILIIAL
                     SLAVGLGVSQQPLILQFAPEWLKNLLSSGIAAGGITAIVLNLIFPPEKQ"
     gene            1447..3156
                     /locus_tag="ACHOSC_RS17550"
                     /old_locus_tag="pgaptmp_003510"
     CDS             1447..3156
                     /locus_tag="ACHOSC_RS17550"
                     /old_locus_tag="pgaptmp_003510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AsmA family protein"
                     /protein_id="HGU4549389.1"
                     /translation="MKFIGKLLLYILIALLVVIAGLYFLLQTRWGAEHISAWVSENSD
                     YHLAFGAMDHRFSAPSHIVLENVTFGRDGQPATLVAKSVDIALSSRQLTEPRHVDTIL
                     LENGTLNLTDQTAPLPFKADRLQLRDMAFNSPNSEWKLSAQRINGGVVPWSPKAGKVL
                     GTKAQIQFSAGSLSLNDVPATNVLIEGSIDNDRVTLTNLGADIARGTLTGNAQRNADG
                     SWQVENLRMADIRLQSEKSLTDFFAPLRSVPSLQIGRLEVIDARLQGPDWAVTDLDLS
                     LRNMTFSKDDWQTQEGKLSMNASEFIYGSLHLFDPIINAEFSPQGVALRQFTSRWEGG
                     MVRTSGNWLRDGKTLILDDVAIAGLEYTLPKNWQQLWMETTPGWLNSLQLKRFSASRN
                     LIIDIDPDFPWQLTALDGYGANLTLVTDHKWGVWSGSANLNAAAATFNRVDVRRPSLA
                     LTANSSTVNISELSAFTEKGILEATASVSQTPQRQTHISLNGRGVPVNILQQWGWPEL
                     PLTGDGNIQLTASGDIQANAPLKPTVSGQLHAVNAAKQQVTQTMNAGIVSSGEVTSAE
                     LVQ"
     gene            complement(3209..5527)
                     /gene="yicI"
                     /locus_tag="ACHOSC_RS17555"
                     /old_locus_tag="pgaptmp_003511"
     CDS             complement(3209..5527)
                     /gene="yicI"
                     /locus_tag="ACHOSC_RS17555"
                     /old_locus_tag="pgaptmp_003511"
                     /EC_number="3.2.1.177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-xylosidase"
                     /protein_id="HGU4549390.1"
                     /translation="MKISDGNWLIQPGLNLIHPLQVFEVEQQGNEMVVYAAPRDVRER
                     TWQLDTPLFTLRFFSPQEGIVGVRIEHFQGALNNGPHYPLNILQDVKVTIENTERYAE
                     FKSGNLSARVSKGEFWSLDFLRNGERITGSQVKNNGYVQDTNNQRNYMFERLDLGVGE
                     TVYGLGERFTALVRNGQTVETWNRDGGTSTEQAYKNIPFYMTNRGYGVLVNHPQCVSF
                     EVGSEKVSKVQFSVESEYLEYFVIDGPTPKAVLDRYTRFTGRPALPPAWSFGLWLTTS
                     FTTNYDEATVNSFIDGMAERNLPLHVFHFDCFWMKAFQWCDFEWDPLTFPDPEGMIRR
                     LKAKGLKICVWINPYIGQKSPVFKELQEKGYLLKRPDGSLWQWDKWQPGLAIYDFTNP
                     DACKWYADKLKGLVAMGVDCFKTDFGERIPTDVQWFDGSDPQKMHNHYAYIYNELVWN
                     VLKDTVGEEEAVLFARSASVGAQKFPVHWGGDCYANYESMAESLRGGLSIGLSGFGFW
                     SHDIGGFENTAPAHVYKRWCAFGLLSSHSRLHGSKSYRVPWAYDDESCDVVRFFTQLK
                     CRMMPYLYREAARANARGTPMMRAMMMEFPDDPACDYLDRQYMLGNNVMVAPVFTEAG
                     DVQFYLPEGRWTHLWHNDELDGSRWHKQQHGFLSLPVYVRDNTLLALGNNDQRPDYVW
                     HEGTAFHLFNLQDGHEAVCEVPAADGSVIFTLKAARTGNTITVTGAGEAKNWTLCLRN
                     VVKVNGLQDGSQAESEQGLVVKPQGNALTITL"
     gene            complement(5537..6919)
                     /locus_tag="ACHOSC_RS17560"
                     /old_locus_tag="pgaptmp_003512"
     CDS             complement(5537..6919)
                     /locus_tag="ACHOSC_RS17560"
                     /old_locus_tag="pgaptmp_003512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418114.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015293 - symporter activity [Evidence
                     IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside-pentoside-hexuronide family
                     transporter"
                     /protein_id="HGU4549391.1"
                     /translation="MKSEVLSVKEKIGYGMGDAASHIIFDNVMLYMMFFYTDIFGIPA
                     GFVGTMFLVARALDAISDPCMGLLADRTRSRWGKFRPWVLFGALPFGIVCVLAYSTPD
                     LSMNGKMIYAAITYTLLTLLYTVVNIPYCALGGVITNDPTQRISLQSWRFVLATAGGM
                     LSTVLMMPLVNLIGGDNKPLGFQGGIAVLSVVAFMMLAFCFFTTKERVEAPPTTTSMR
                     EDLRDIWQNDQWRIVGLLTIFNILAVCVRGGAMMYYVTWILGTPEVFVAFLTTYCVGN
                     LIGSALAKPLTDWKCKVTIFWWTNALLAVISLAMFFVPMQASITMFVFIFVIGVLHQL
                     VTPIQWVMMSDTVDYGEWCNGKRLTGISFAGTLFVLKLGLAFGGALIGWMLAYGGYDA
                     AEKAQNSATISIIIALFTIVPAICYLLSAIIAKRYYSLTTHNLKTVMEQLAQGKRRCQ
                     QQFTSQEVQN"
     gene            7212..7306
                     /locus_tag="ACHOSC_RS17565"
                     /old_locus_tag="pgaptmp_003513"
     tRNA            7212..7306
                     /locus_tag="ACHOSC_RS17565"
                     /old_locus_tag="pgaptmp_003513"
                     /product="tRNA-Sec"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:7246..7248,aa:Sec,seq:tca)
     gene            7469..>8622
                     /locus_tag="ACHOSC_RS17570"
                     /old_locus_tag="pgaptmp_003514"
     CDS             7469..>8622
                     /locus_tag="ACHOSC_RS17570"
                     /old_locus_tag="pgaptmp_003514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001218976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HGU4549392.1"
                     /translation="MALTDIQIKRAKPQDKPYTLNDGQGLSLLINPDGSKGWRFRFRF
                     AGKARLMSFGSYDLVSLAEAREKRDIARKQVANGIDPVEERKAQKLAQKLSTKNSFES
                     VSREWHAAKADRWTLAYREEIIKTFEQDVFPFIGKRPIAEIKPLELLEVLKRIEKRGA
                     LEKTRKVRQRCGEVFRYAIITGRAEYNPAPDLAIALAVPKQKHHPFLSAEELPHFVRD
                     LEAYTGSIITKNATKIVMLTGVRTQEMRFATWQELDLEKGLWEIPAERMKMRRPHIVP
                     LSRQVIRLFQQLKPITGHYPYIFIGRNNRKKPISKESINQVIELLGFKGRITGHGFRH
                     TMSTILHEAGFNSAWIEMQLAHVDKNSIRGVYNHAQYLNGRSRMLQFYAD"
BASE COUNT         2179 a         2222 c         2149 g         2072 t
ORIGIN      
        1 atttaccgtc ttgaagatcg tccgccgctt cctcaaaccc tgtttgccgc ctgtcagcat
       61 ctgctggcga tgttcgttgc ggtgatcacg ccagcgctat taatctgcca ggcgctgggt
      121 ttaccggcac aagacacgca acacattatt agtatgtcgc tgtttgcctc cggtgtggca
      181 tcgattattc aaattaaggc ctggggtccg gttggctccg ggctgttgtc tattcagggc
      241 accagcttca actttgttgc cccgctgatt atgggcggta ccgcgctgaa aaccggtggt
      301 gctgatgttc ctaccatgat ggcggctttg ttcggcacgt tgatgctggc aagttgcacc
      361 gagatggtga tctcccgcgt tctgcatctg gcgcgccgca ttattacgcc gctggtttct
      421 ggcgttgtgg tgatgattat cggcctgtcg ctaattcagg ttgggttaac gtccattggc
      481 ggcggttacg cagccatgag cgataacacc ttcggcgcac cgaaaaatct gctgctggca
      541 ggcgtggtct tagccttaat tatcctgctt aaccgtcaac gtaaccctta cttacgcgtg
      601 gcctcactgg tgattgcgat ggcggccgga tatgcgctgg cgtggtttat gggcatgttg
      661 ccagaaagca acgaaccgat gacgcaagaa ctgattatgg tgccaacgcc gctctattac
      721 ggtcttggca ttgaatggag tctgctgctg ccgctgatgc tggtctttat gatcacttcg
      781 ctggaaacca ttggcgatat cacggcgacc tctgacgttt ccgaacagcc agtgtccggt
      841 ccgctgtaca tgaaacgcct gaaaggcggc gtgctggcaa acggcctgaa ctcgtttgtt
      901 tcggcggtgt ttaacacctt cccgaactcc tgcttcgggc aaaacaacgg agtgatccag
      961 ttgactggtg ttgccagccg ctatgtcggt tttgtcgtcg cgctgatgtt gatcgtgctg
     1021 ggtctgttcc cggcagtgag cggttttgta caacacattc cagaaccggt tctgggcggc
     1081 gcaacgcttg taatgtttgg cactatcgcc gcctccggtg tgcgtatcgt ttctcgtgaa
     1141 ccgctgaacc gtcgggcgat tctgattatc gcgctgtcgc tggcggttgg tctgggcgtg
     1201 tctcagcagc cgctgatttt gcagtttgcc cctgaatggc tgaaaaacct gctctcctcc
     1261 gggatcgccg cgggcggtat tactgccatc gtgctgaatc tgattttccc accagaaaaa
     1321 cagtaattca ctggcggcgg taagataatt accgccgcag tctcacctct ttcaccattc
     1381 ccgccttgag gattgcgcgt aaatcgtgca taactccctt atgtgcattt cacgggatgg
     1441 aagaccatga aatttattgg gaagctgctt ctctacattc tcatcgctct gttagtggtg
     1501 atcgctggcc tctattttct tctgcaaacc cgctggggag cagaacatat cagcgcatgg
     1561 gtttccgaga atagcgacta tcatctggcc ttcggggcga tggatcaccg tttttccgcg
     1621 ccatctcata tcgtgctgga gaacgtcacg tttggccgtg atggccagcc cgcgaccctg
     1681 gtggcaaaaa gtgtcgacat tgcgctaagc agtcggcaac tgaccgaacc acgccatgtc
     1741 gataccatcc tgctggaaaa cgggacgctg aatctcaccg accagaccgc gccgctaccg
     1801 ttcaaagccg atcgtctgca actgcgtgat atggcgttta atagcccgaa tagcgaatgg
     1861 aaactgagcg cgcagcggat aaatggcggc gtagttccgt ggtcaccaaa agccggtaaa
     1921 gtgctgggta cgaaggcgca gattcagttt agtgccggat cgctttcgct caatgatgtt
     1981 cctgccacca atgtactgat tgaaggcagt attgataacg atcgcgttac gctgactaac
     2041 ctgggtgccg acatcgcccg cgggacatta accggaaacg cgcagcgtaa cgccgacggc
     2101 agctggcaag tggaaaacct gcgcatggcg gatatacgtc tacaaagcga aaaatcgcta
     2161 accgacttct ttgcgccatt acgctctgtc ccgtcgttgc agattggtcg cctggaagtg
     2221 atcgatgctc gtttgcaagg tccggactgg gcggtgaccg acctcgatct cagcttgcgc
     2281 aacatgacct tcagtaaaga tgactggcag acacaggaag gcaaactgtc gatgaacgct
     2341 agcgagttca tttatggttc gctgcattta tttgacccga ttataaacgc ggaattttcc
     2401 ccgcagggcg tagcgctgcg ccagttcacc agccgctggg aagggggtat ggtcagaacg
     2461 tcagggaact ggctgcgtga cgggaaaacg ttgatccttg atgatgtggc aattgccggg
     2521 ctggaatata ccttgccgaa aaactggcaa cagttgtgga tggaaacgac acccggttgg
     2581 ttaaacagcc tgcaactgaa gagatttagc gccagccgca atctgatcat tgatatcgac
     2641 cctgacttcc cgtggcagct caccgcgctc gatggttacg gtgccaacct gacgctggtt
     2701 accgatcata aatggggcgt ctggagtggc tcggcgaatc tgaatgccgc cgccgcgaca
     2761 ttcaatcgtg ttgatgttcg tcgcccgtcg ctggcgctga ccgccaacag cagcacggtg
     2821 aatatcagcg aactgagtgc atttactgaa aaaggcattc tggaagccac tgccagtgtt
     2881 tcacaaacgc cacaacgtca gacccatatc agcctgaatg gacgcggtgt gccggtgaat
     2941 attttgcaac aatggggatg gcctgaatta ccgttgactg gcgacggcaa tattcagctt
     3001 accgccagtg gagatattca ggccaatgcc ccgctgaaac ctacggttag cgggcaattg
     3061 catgccgtga acgccgcaaa gcagcaagtg actcaaacca tgaatgctgg catcgtttcc
     3121 agcggtgaag ttacgtcggc agagctggtg cagtaaacat caatctcgcc gggtacgact
     3181 cagacgtacc cggcattcca tcaatagatt acaacgtaat tgtcagcgca ttcccttgag
     3241 gcttcaccac cagcccctgc tcactttcag cctgcgaacc gtcttgcaga ccatttactt
     3301 tcacaacatt gcgcaggcac agtgtccagt tcttcgcctc gcccgcacca gtcacagtaa
     3361 tcgtgttgcc agtacgtgct gcttttaaag taaagatcac cgatccgtca gcagcgggca
     3421 cttcacagac ggcttcatgc ccgtcttgca gattaaagag gtggaatgcc gtgccttcgt
     3481 gccacacgta atcgggacgt tgatcgttgt tgcccagcgc cagtagagtg ttatcacgca
     3541 cataaacggg cagactcagg aagccgtgct gctgtttatg ccagcgacta ccgtcgagtt
     3601 catcgttgtg ccacaggtgt gtccagcgac cttccggcag gtagaactgc acatcgcccg
     3661 cttcagtgaa caccggcgca accatcacgt tgttgcctaa catgtattga cggtcaaggt
     3721 aatcacaagc cggatcgtcc gggaactcca tcatcatggc ccgcatcatc ggcgtacccc
     3781 gcgcgttcgc acgcgcagct tcacgataca gatacggcat catgcggcat ttcagttgcg
     3841 tgaagaagcg cactacatca caggactcat catcgtaggc ccacggcaca cgataagatt
     3901 tgctaccgtg taaacggcta tggctggaga gcaaaccaaa cgcgcaccag cgtttgtaaa
     3961 cgtgcgccgg agcggtattt tcaaagccgc cgatatcgtg gctccagaag ccaaaacctg
     4021 aaaggccaat agacaaacca ccgcgcaggc tttccgccat tgattcgtag ttagcgtaac
     4081 aatcgccacc ccagtgtacc gggaatttct gcgcaccgac ggaggccgag cgggcaaaca
     4141 agacagcttc ttcctcacca acggtgtcct tgagcacgtt ccacaccagt tcgttgtaga
     4201 tgtatgcata atggttatgc attttctgcg gatcggaacc gtcaaaccac tgaacatcag
     4261 ttgggatacg ttcgccaaag tcggtcttaa agcaatcaac gcccatcgcg accagacctt
     4321 tcagtttgtc ggcgtaccat ttgcaggcat ccggattggt aaagtcataa atcgccagac
     4381 ctggctgcca tttatcccac tgccatagcg aaccgtccgg gcgtttgagt aaatagcctt
     4441 tctcttgtaa ctctttaaag acgggggatt tttgaccgat ataggggtta atccagacgc
     4501 agattttcag ccctttcgct ttcaggcggc ggatcatccc ttccgggtca gggaaagtca
     4561 gcgggtccca ctcaaaatcg caccactgga aggctttcat ccagaaacag tcaaagtgga
     4621 aaacatgcag cggcagattg cgttccgcca taccatcgat aaagctgttt accgtcgctt
     4681 cgtcgtagtt ggtggtaaat gaagtggtta gccacaggcc gaaggaccac gcgggcggca
     4741 gcgccggacg accagtaaag cgggtataac gatcaagtac cgctttcggc gtcgggccgt
     4801 cgataacaaa gtattcgaga tattcactct caacgctgaa ctgcactttg gagactttct
     4861 ccgatcccac ttcaaaagag acacactggg gatgattgac cagtacccca taaccacggt
     4921 tagtcatgta gaacgggata tttttatacg cctgttcagt acttgtgccg ccgtcccggt
     4981 tccaggtctc taccgtctgg ccattgcgca ccagggcagt aaagcgctct cccagaccgt
     5041 aaactgtttc gccaacgcca agatcaagcc gctcaaacat ataattgcgt tgattattcg
     5101 tgtcctgcac gtagccatta tttttcacct gactaccggt aatacgttcg ccgttgcgca
     5161 gaaaatccag tgaccagaac tcacctttgc tgacacgcgc gcttaagttg ccacttttaa
     5221 actcagcata acgttctgtg ttttcgattg tgaccttcac gtcctgcaaa atattgagcg
     5281 gataatgagg accgttattc agcgccccct gaaaatgctc aatccgcaca ccgacaatac
     5341 cttcctgtgg ggagaaaaag cgcagcgtaa ataaaggcgt atcaagctgc caggtacgtt
     5401 cacgcacatc acggggggca gcatagacca ccatttcatt accctgctgt tcaacctcga
     5461 atacctgaag cgggtgaatc aaattgaggc caggttgaat caaccagttt ccatcgctaa
     5521 ttttcattgc cgttccttag ttctgcactt cttgagaggt gaattgttgc tggcaacggc
     5581 gtttaccctg agccagctgt tccataacgg ttttcagatt gtgcgtcgtg agtgagtagt
     5641 agcgtttagc gataatcgcg ctcagcaaat aacagatcgc cggaacaatc gtgaatagcg
     5701 caataatgat gctaatcgtg gcgctgttct gcgctttttc tgccgcatca tatccgccat
     5761 aagccagcat ccagccgata agagcgccgc cgaaggccaa ccccagtttg agcacaaaca
     5821 gcgtgccagc aaaactgatc ccggtcaggc gtttaccatt gcaccactcg ccgtagtcga
     5881 cggtatcgga catcattacc cactggatag gtgtcaccag ttgatgcaac acaccaatca
     5941 cgaagatgaa gacaaacata gtgatgctgg cctgcatggg aacaaagaac atcgcgaggc
     6001 taatcactgc cagcagggcg ttcgtccacc agaagatagt gactttacat ttccagtcgg
     6061 tcagaggttt tgccagtgcg gaaccaatca ggttacccac gcaataagtg gtgagaaaag
     6121 cgacaaacac ttccggcgtg cccaaaatcc atgtgacgta atacatcatc gccccaccgc
     6181 gtacgcacac cgccaggata ttgaaaatgg ttagtaaacc gacaatccgc cactggtcgt
     6241 tttgccagat atcacgtaaa tcttcccgca tagacgttgt tgtaggtggt gcttcaacgc
     6301 gttctttagt ggtgaagaaa cagaatgcca gcatcatgaa tgccaccacg gaaaggaccg
     6361 cgataccgcc ctggaaaccg agtggtttat tatcaccgcc aattaaatta accagtggca
     6421 tcatcagaac agtagaaagc atgcctcccg cggtcgccag cacaaaacgc caggattgca
     6481 gcgagatacg ctgagtcggg tcattggtga ttacaccacc caatgcgcag taagggatat
     6541 tgacgacggt ataaagtaag gtaagtaggg tgtaagtaat tgctgcatag atcattttgc
     6601 cgttcatact gagatctggc gtgctatagg ccagtacaca gacgatcccg aatggcagtg
     6661 cgccaaacag tacccacgga cgaaatttac cccagcgaga gcgcgttcga tcggccaaca
     6721 accccatgca aggatcggaa atcgcatcca gtgcacgagc gaccaaaaac atggttccga
     6781 caaaaccggc aggaatgcca aaaatatcgg tataaaagaa catcatatat aacattacgt
     6841 tatcgaaaat aatgtggctg gcggcgtctc ccatgccata accaattttc tctttaacgg
     6901 acaacacttc actcttcatc tgcttttcct tgatgctata ggggtgctga gaccgataac
     6961 gcattttgta aaccatccct gtgagagcga atattccgat atctggttat taatttatga
     7021 ttcttgtttt atgtgatcgt ggtagcgtta atttcgctca tatatcattg taaaatatag
     7081 gttttatatg aactataatg ctttcgtgat aatacgctgc gtgtattagg cggaaaaaac
     7141 tgatctgggg gatgtagaaa ctcaaggaag tagctataat gcgccccgcc tccatgtagc
     7201 aatcgaggcg cggaagatcg tcgtctccgg tgaggcggct ggacttcaaa tccagttggg
     7261 gccgccagcg gtcccgggca ggttcgactc ctgtgatctt ccgccaaacc tcatccagcc
     7321 agttccgagg cactccataa aatcctttat attcaatgcg atgaataatc agcttatccg
     7381 taatagtccg agatcatctc cctgaatccg cgaaaaatat gtatagtaat gtgtatagat
     7441 tttttatacg cagttttcag ttatacacat ggctctaacc gatatacaga tcaaacgtgc
     7501 aaaaccacaa gacaaaccat acacattgaa cgatggacaa ggtctgtcat tgcttatcaa
     7561 tcctgatggc tcgaaaggct ggcgtttccg tttccggttt gcagggaaag cgcgtttaat
     7621 gtcatttggc agctacgatt tagtaagcct cgcagaagca cgtgaaaagc gtgatatcgc
     7681 ccgtaagcag gttgctaatg gcattgaccc ggtagaagaa cgtaaagctc agaaacttgc
     7741 tcagaaatta tccactaaaa actcttttga gagcgttagc agagaatggc atgcagcaaa
     7801 agcagatcgt tggaccttag cttatcgaga agaaattatt aaaacatttg aacaggatgt
     7861 ttttcctttc attggtaaac gaccaatagc tgaaattaaa cccctagaac tgcttgaagt
     7921 acttaaacgc atagaaaaac gtggagcgtt ggagaaaaca agaaaagtac gccaacggtg
     7981 cggagaagtg tttcgctacg cgatcatcac tggccgcgct gaatacaatc cagcacctga
     8041 tttagctatc gcattagcag tgccgaagca aaaacatcat ccttttctat cagcagagga
     8101 actcccacat tttgtcagag atcttgaggc atacactggc agcataatca ctaaaaatgc
     8161 aaccaagatt gtgatgctta caggtgttcg aacacaagag atgcgctttg ctacatggca
     8221 agagcttgat ttagaaaaag ggctatggga gataccagca gagcgtatga agatgcgtag
     8281 gccacacata gtcccgcttt caaggcaggt tataaggcta tttcaacaac taaaaccgat
     8341 cactggtcac tatccttaca ttttcattgg acgaaataac cgaaaaaagc ctataagtaa
     8401 ggagagcata aaccaagtga ttgagttatt gggatttaag gggagaatca ctggccatgg
     8461 tttcagacac actatgtcaa ccatactaca cgaagccggt tttaatagtg catggattga
     8521 aatgcaactt gcccacgttg ataagaacag cattcgcggt gtatacaatc atgcacaata
     8581 tcttaatggt cgatctcgta tgcttcagtt ttatgcagat aa
//
LOCUS       DAZKHF020000187         8600 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2201S31241
            SAMN43267800-rid25106413.denovo.188, whole genome shotgun sequence.
ACCESSION   DAZKHF020000187 DAZKHF020000000
VERSION     DAZKHF020000187.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267800
            Sequence Read Archive: SRR30307263
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 8600)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8600)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 8600)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350581.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350581.2
            Genome Coverage       :: 151x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:21:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,652
            CDSs (total)                      :: 5,557
            Genes (coding)                    :: 5,320
            CDSs (with protein)               :: 5,320
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 237
            CDSs (without protein)            :: 237
            Pseudo Genes (ambiguous residues) :: 0 of 237
            Pseudo Genes (frameshifted)       :: 102 of 237
            Pseudo Genes (incomplete)         :: 130 of 237
            Pseudo Genes (internal stop)      :: 52 of 237
            Pseudo Genes (multiple problems)  :: 41 of 237
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8600
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267800-rid25106413.denovo.188"
                     /serotype="O 26:H11"
                     /isolate="2201S31241"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-01"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            complement(258..521)
                     /gene="rpsT"
                     /locus_tag="ACHOSC_RS17575"
                     /old_locus_tag="pgaptmp_003515"
     CDS             complement(258..521)
                     /gene="rpsT"
                     /locus_tag="ACHOSC_RS17575"
                     /old_locus_tag="pgaptmp_003515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006117633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S20"
                     /protein_id="HGU4549393.1"
                     /translation="MANIKSAKKRAIQSEKARKHNASRRSMMRTFIKKVYAAIEAGDK
                     AAAQKAFNEMQPIVDRQAAKGLIHKNKAARHKANLTAQINKLA"
     gene            624..842
                     /locus_tag="ACHOSC_RS17580"
                     /old_locus_tag="pgaptmp_003516"
     CDS             624..842
                     /locus_tag="ACHOSC_RS17580"
                     /old_locus_tag="pgaptmp_003516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2575 family protein"
                     /protein_id="HGU4549394.1"
                     /translation="MCRHSLRSDGAGFYQLAGCEYSFSAIKIAAGGHFLPVICAMAMK
                     SHFFLISVLNRRLTLTAVQGILGRFSLF"
     gene            850..1791
                     /gene="ribF"
                     /locus_tag="ACHOSC_RS17585"
                     /old_locus_tag="pgaptmp_003517"
     CDS             850..1791
                     /gene="ribF"
                     /locus_tag="ACHOSC_RS17585"
                     /old_locus_tag="pgaptmp_003517"
                     /EC_number="2.7.1.26"
                     /EC_number="2.7.7.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003919 - FMN adenylyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional riboflavin kinase/FAD synthetase"
                     /protein_id="HGU4549395.1"
                     /translation="MKLIRGIHNLSQAPQEGCVLTIGNFDGVHRGHRALLQGLQEEGR
                     KRNLPVMVMLFEPQPLELFATDKAPARLTRLREKLRYLAECGVDYVLCVRFDRRFAAL
                     TAQNFISDLLVKHLRVKFLAVGDDFRFGAGREGDFLLLQKAGMEYGFDITSTQTFCEG
                     GVRISSTAVRQALADDNLALAESLLGHPFAISGRVVHGDELGRTIGFPTANVPLRRQV
                     SPVKGVYAVEVLGLGEKPLPGVANIGTRPTVAGIRQQLEVHLLGVAMDLYGRHIQVVL
                     RKKIRNEQRFASLDELKAQIARDELTAREFFGLTKPA"
     gene            1834..4650
                     /gene="ileS"
                     /locus_tag="ACHOSC_RS17590"
                     /old_locus_tag="pgaptmp_003518"
     CDS             1834..4650
                     /gene="ileS"
                     /locus_tag="ACHOSC_RS17590"
                     /old_locus_tag="pgaptmp_003518"
                     /EC_number="6.1.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004822 - isoleucine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006428 - isoleucyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isoleucine--tRNA ligase"
                     /protein_id="HGU4549396.1"
                     /translation="MSDYKSTLNLPETGFPMRGDLAKREPGMLARWTDDDLYGIIRAA
                     KKGKKTFILHDGPPYANGSIHIGHSVNKILKDIIVKSKGLSGYDSPYVPGWDCHGLPI
                     ELKVEQEYGKPGEKFTAAEFRAKCREYAATQVDGQRKDFIRLGVLGDWSHPYLTMDFK
                     TEANIIRALGKIIGNGHLHKGAKPVHWCVDCRSALAEAEVEYYDKTSPSIDVAFQAVD
                     QDALKAKFAVSNVNGPISLVIWTTTPWTLPANRAISIAPDFDYALVQIDGQAVILAKD
                     LVESVMQRIGVTDYTILGTVKGAELELLRFTHPFMGFDVPAILGDHVTLDAGTGAVHT
                     APGHGPDDYVIGQKYGLETANPVGPDGTYLPGTYPTLDGVNVFKANDIVVALLQEKGA
                     LLHVEKMQHSYPCCWRHKTPIIFRATPQWFVSMDQKGLRAQSLKEIKGVQWIPDWGQA
                     RIESMVANRPDWCISRQRTWGVPMSLFVHKDTEELHPRTLELMEEVAKRVEVDGIQAW
                     WDLDAKEILGDEADQYVKVPDTLDVWFDSGSTHSSVVDVRPEFAGHAADMYLEGSDQH
                     RGWFMSSLMISTAMKGKAPYRQVLTHGFTVDGQGRKMSKSIGNTVSPQDVMNKLGADI
                     LRLWVASTDYTGEMAVSDEILKRAADSYRRIRNTARFLLANLNGFDPAKDMVKPEEMV
                     VLDRWAVGCAKAAQEDILKAYEAYDFHEVVQRLMRFCSVEMGSFYLDIIKDRQYTAKA
                     DSVARRSCQTALYHIAEALVRWMAPILSFTADEVWGYLPGEREKYVFTGEWYEGLFGL
                     ADSEAMNDAFWDELLKVRGEVNKVIEQARADKKVGGSLEAAVTLYAEPELAAKLTALG
                     DELRFVLLTSGATVADYNDAPADAQQSEVLKGLKVALSKAEGEKCPRCWHYTQDVGKV
                     AEHAEICGRCVSNVAGDGEKRKFA"
     gene            4650..5144
                     /gene="lspA"
                     /locus_tag="ACHOSC_RS17595"
                     /old_locus_tag="pgaptmp_003519"
     CDS             4650..5144
                     /gene="lspA"
                     /locus_tag="ACHOSC_RS17595"
                     /old_locus_tag="pgaptmp_003519"
                     /EC_number="3.4.23.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0004190 - aspartic-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase II"
                     /protein_id="HGU4549397.1"
                     /translation="MSQSICSTGLRWLWLVVVVLIIDLGSKYLILQNFALGDQVPLFP
                     SLNLHYARNYGAAFSFLADSGGWQRWFFAGIAIGISVILAVMMYRSKATQKLNNIAYA
                     LIIGGALGNLFDRLWHGFVVDMIDFYVGDWHFATFNLADTAICVGAALIVLEGFLPSK
                     AKKQ"
     gene            5232..5681
                     /gene="fkpB"
                     /locus_tag="ACHOSC_RS17600"
                     /old_locus_tag="pgaptmp_003520"
     CDS             5232..5681
                     /gene="fkpB"
                     /locus_tag="ACHOSC_RS17600"
                     /old_locus_tag="pgaptmp_003520"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FKBP-type peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HGU4549398.1"
                     /translation="MSESVQSNSAVLVHFTLKLDDGTTAESTRNNGKPALFRLGDASL
                     SEGLEQHLLGLKVGDKTTFSLEPDAAFGVPSPDLIQYFSRREFMDAGEPEIGAIMLFT
                     AMDGSEMPGVIREINGDSITVDFNHPLAGQTVHFDIEVLEIDPALEA"
     gene            5683..6633
                     /gene="ispH"
                     /locus_tag="ACHOSC_RS17605"
                     /old_locus_tag="pgaptmp_003521"
                     /gene_synonym="lytB"
     CDS             5683..6633
                     /gene="ispH"
                     /locus_tag="ACHOSC_RS17605"
                     /old_locus_tag="pgaptmp_003521"
                     /gene_synonym="lytB"
                     /EC_number="1.17.7.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051745 - 4-hydroxy-3-methylbut-2-enyl
                     diphosphate reductase activity [Evidence IEA];
                     GO_process: GO:0019288 - isopentenyl diphosphate
                     biosynthetic process, methylerythritol 4-phosphate pathway
                     [Evidence IEA];
                     GO_process: GO:0050992 - dimethylallyl diphosphate
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-3-methylbut-2-enyl diphosphate
                     reductase"
                     /protein_id="HGU4549399.1"
                     /translation="MQILLANPRGFCAGVDRAISIVENALAIYGAPIYVRHEVVHNRY
                     VVDSLRERGAIFIEQISEVPDGAILIFSAHGVSQAVRNEAKSRDLTVFDATCPLVTKV
                     HMEVARASRRGEESILIGHAGHPEVEGTMGQYSNPEGGMYLVESPDDVWKLTVKNEEK
                     LSFMTQTTLSVDDTSDVIDALRKRFPKIVGPRKDDICYATTNRQEAVRALAEQAEVVL
                     VVGSKNSSNSNRLAELAQRMGKRAFLIDDAKDIQEEWVKEVKCVGVTAGASAPDILVQ
                     NVVARLQQLGGGEAIPLEGREENIVFEVPKELRVDIREVD"
     gene            6699..7613
                     /gene="rihC"
                     /locus_tag="ACHOSC_RS17610"
                     /old_locus_tag="pgaptmp_003522"
     CDS             6699..7613
                     /gene="rihC"
                     /locus_tag="ACHOSC_RS17610"
                     /old_locus_tag="pgaptmp_003522"
                     /EC_number="3.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016799 - hydrolase activity, hydrolyzing
                     N-glycosyl compounds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonucleoside hydrolase RihC"
                     /protein_id="HGU4549400.1"
                     /translation="MRLPIFLDTDPGIDDAVAIAAAIFAPELDLQLMTTVAGNVSVEK
                     TTRNALQLLHFWNAEIPLAQGAAVPLVRAPRDAASVHGESGMAGYDFVEHNRKPLGIP
                     AFLAIRDALMRAPEPVTLVAIGPLTNIALLLSQCPECKPYIRRLVIMGGSAGRGNCTP
                     NAEFNIAADPEAAACVFRSGIEIVMCGLDVTNQAILTPDYLSTLPQLNRTGKMLHALF
                     SHYRSGSMQSGLRMHDLCAIAWLVRPDLFTLKPCFVAVETQGEFTSGTTVVDIDGCLG
                     KPANVQVALDLDVKGFQQWVAEVLALAS"
     gene            7780..>8600
                     /gene="dapB"
                     /locus_tag="ACHOSC_RS17615"
                     /old_locus_tag="pgaptmp_003523"
     CDS             7780..>8600
                     /gene="dapB"
                     /locus_tag="ACHOSC_RS17615"
                     /old_locus_tag="pgaptmp_003523"
                     /EC_number="1.17.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008839 - 4-hydroxy-tetrahydrodipicolinate
                     reductase [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate reductase"
                     /protein_id="HGU4549401.1"
                     /translation="MHDANIRVAIAGAGGRMGRQLIQAALALEGVQLGAALEREGSSL
                     LGSDAGELAGAGKTGVTVQSSLDAVKDDFDVFIDFTRPEGTLNHLAFCRQHGKGMVIG
                     TTGFDEAGKQAIRDAAADIAIVFAANFSVGVNVMLKLLEKAAKVMGDYTDIEIIEAHH
                     RHKVDAPSGTALAMGEAIAHALDKDLKDCAVYSREGHTGERVPGTIGFATVRAGDIVG
                     EHTAMFADIGERLEITHKASSRMTFANGAVRSALWLSGKESGLFDMRDVLDLNSL"
BASE COUNT         1942 a         2148 c         2461 g         2049 t
ORIGIN      
        1 tgtgtatcac aatatccaca ttctttgtag gggagtctgg agattgagta gatattcttg
       61 ttcagaatgt atcagccgat ggttctacga ttcttaagcc acgaagagtt cagaaagtgt
      121 aacggcatgt ctgcaaaagc cagcatcttt tgactatctg gcaaccggta gtgtgttctc
      181 tcacgcatca taaaagcagc aggcataaaa aaacccgctt gcgcgggctt tttcacaaag
      241 cttcagcaaa ttggcgatta agccagtttg ttgatctgtg cagtcaggtt agccttatga
      301 cgtgcagctt tgtttttgtg gatcagacct ttagcagcct gacggtccac gatcggttgc
      361 atttcgttaa atgctttctg tgcagcagct ttgtcgccag cttcgatagc tgcgtatact
      421 ttcttgatga aagtacgcat catagagcga cggcttgcgt tgtgcttacg agccttttca
      481 gactgaatgg cgcgcttctt agctgatttg atattagcca aggtccaact cccaaatgtg
      541 ttctatatgg acaattcaaa ggccgaggaa tatgcccttt tagccttctt ttgtcaatgg
      601 atttgtgcaa ataagcgccg ttaatgtgcc ggcactcgtt acgtagtgat ggcgcaggat
      661 tctaccagct tgcagggtgt gaatacagct tttccgcgat aaaaattgca gcaggcggtc
      721 actttcttcc cgtgatttgc gccatggcaa tgaaaagcca cttctttctg atttcggtac
      781 tcaatcgccg gttaaccttg accgctgtac aaggtatact cggacgattt tcactgtttt
      841 gagccagaca tgaagctgat acgcggcata cataatctca gccaggcccc gcaagaaggg
      901 tgtgtgctga ctattggtaa tttcgacggc gtgcatcgcg gtcatcgcgc gctgttacag
      961 ggcttgcagg aagaagggcg caagcgcaac ttaccggtga tggtgatgct ttttgaacct
     1021 caaccactgg aactgtttgc taccgataaa gccccggcac ggctgacccg gctgcgggaa
     1081 aaactgcgtt accttgcaga gtgtggcgtt gattacgtgc tgtgcgtgcg tttcgacagg
     1141 cgtttcgcgg cgttaaccgc gcaaaatttc atcagcgatc ttctggtgaa gcatttgcgc
     1201 gtaaaatttc ttgccgtagg tgatgatttc cgctttggcg ctggtcgtga aggcgatttc
     1261 ttgttattac agaaagctgg catggaatac ggcttcgata tcaccagtac gcaaactttt
     1321 tgcgaaggtg gcgtgcgtat cagcagcacc gctgtgcgtc aggcgctggc ggatgacaat
     1381 ctggctctgg cagaaagttt actggggcac ccgtttgcca tctccgggcg tgtagtccac
     1441 ggtgatgaat tagggcgcac tataggtttc ccgacggcga atgtaccgct acgccgtcag
     1501 gtttccccgg tgaaaggggt ttatgcggta gaagtgctgg gcctcggtga aaagccgtta
     1561 cccggcgtgg caaacattgg aacacgccca acggttgccg gtattcgcca gcagctggaa
     1621 gtgcatttgt taggtgttgc aatggacctt tacggtcgcc atatacaagt agtgctgcgt
     1681 aaaaaaatac gcaatgagca gcgatttgcg tcgctggacg aactgaaagc gcagattgcg
     1741 cgtgatgaat taaccgcccg cgaatttttt gggctaacaa aaccggctta agcctgttat
     1801 gtaatcaaac cgaaacacgg aaccgagaat ctgatgagtg actataaatc aaccctgaat
     1861 ttgccggaaa cagggttccc gatgcgtggc gatctcgcca agcgcgaacc gggaatgctg
     1921 gcgcgttgga ctgatgatga tctgtacggc atcatccgtg cggctaaaaa aggcaaaaaa
     1981 accttcattc tgcatgatgg ccctccttat gcgaatggca gcattcatat tggtcactcg
     2041 gttaacaaga ttctgaaaga cattatcgtg aagtccaaag ggctttccgg ttatgactcg
     2101 ccgtatgtgc ctggctggga ctgccacggt ctgccgatcg agctgaaagt agagcaagaa
     2161 tacggtaagc cgggtgagaa attcaccgcc gccgagttcc gcgccaagtg ccgcgaatac
     2221 gcggcgaccc aggttgacgg tcaacgcaaa gactttatcc gtctgggcgt gctgggcgac
     2281 tggtcgcacc cgtacctgac catggacttc aaaactgaag ccaacatcat ccgcgcgctg
     2341 ggcaaaatca tcggcaacgg tcacctgcac aaaggcgcga agccagttca ctggtgcgtt
     2401 gactgccgtt ctgcgctggc ggaagcggaa gttgagtatt acgacaaaac ttctccgtcc
     2461 atcgacgttg cttttcaggc ggtcgatcag gatgcactga aagcaaaatt tgccgtaagc
     2521 aacgttaacg gcccaatctc gctggtgatc tggaccacta cgccgtggac tctgcctgcg
     2581 aaccgcgcaa tctctattgc acctgatttc gactatgcgc tggtgcagat cgacggtcag
     2641 gccgtgattc tggcgaaaga tctggttgaa agcgtaatgc agcgtatcgg cgtgaccgat
     2701 tacaccattc tcggcacggt aaaaggtgcg gagcttgagt tgctgcgctt tacccatccg
     2761 tttatgggct tcgacgttcc ggcaatcctc ggcgatcacg ttaccctgga tgccggtacc
     2821 ggtgccgttc acaccgcgcc tggccacggc ccggacgact atgtgatcgg tcagaaatac
     2881 ggcctggaaa ccgctaaccc ggttggcccg gacggcactt atctgccggg cacttatccg
     2941 acgctggatg gcgtgaacgt cttcaaagcg aacgacatcg tcgttgcgct gctgcaggaa
     3001 aaaggcgcgc tgctgcacgt tgagaaaatg cagcacagct atccgtgctg ctggcgtcac
     3061 aaaacgccga tcatcttccg cgcgacgccg cagtggttcg tcagcatgga tcagaaaggt
     3121 ctgcgtgcgc agtcactgaa agagatcaaa ggcgtgcagt ggatcccgga ctggggccag
     3181 gcgcgtatcg agtcgatggt tgctaaccgt cctgactggt gtatctcccg tcagcgcacc
     3241 tggggcgtac cgatgtcact gttcgtgcac aaagacacgg aagagctgca tccgcgtact
     3301 ctcgaactaa tggaagaagt ggcaaaacgc gttgaagttg acggcatcca ggcgtggtgg
     3361 gatcttgatg cgaaagagat cctcggcgat gaagctgatc agtacgtgaa agtgccggac
     3421 acattggatg tatggtttga ctccggatct acccactctt ctgttgttga cgtgcgtccg
     3481 gaatttgccg gtcacgcagc ggacatgtat ctggaaggtt ctgaccagca ccgtggttgg
     3541 ttcatgtctt ccctaatgat ctccaccgcg atgaaaggca aagcgccgta tcgtcaggta
     3601 ctgacccacg gctttaccgt ggatggtcag ggtcgcaaga tgtctaaatc catcggcaat
     3661 accgtttcgc cgcaggatgt gatgaacaaa ctgggcgcgg atattctgcg tctgtgggtg
     3721 gcatcaaccg actacaccgg tgaaatggcc gtttctgacg agatcctgaa acgtgctgcc
     3781 gatagctatc gtcgtatccg taacaccgcg cgcttcctgc tggcaaacct gaacggtttt
     3841 gatccagcaa aagatatggt gaaaccggaa gagatggtgg tactggatcg ctgggccgta
     3901 ggttgtgcga aagcggcaca ggaagacatc ctcaaggcgt acgaagcata cgatttccac
     3961 gaagtggtac agcgtctgat gcgcttctgc tccgttgaga tgggttcctt ctacctcgac
     4021 atcatcaaag accgtcagta caccgccaaa gcggacagcg tggcgcgtcg tagctgccag
     4081 actgcgctgt atcacatcgc tgaagcgctg gtgcgctgga tggcaccaat cctctccttc
     4141 accgctgatg aagtgtgggg ctatctgccg ggcgaacgtg aaaaatacgt ctttaccggt
     4201 gagtggtacg aaggcctgtt tggtctggca gacagtgaag cgatgaacga tgcgttctgg
     4261 gacgagctgt tgaaagtgcg tggcgaagtg aacaaagtca ttgagcaagc gcgtgccgat
     4321 aagaaagtgg gtggctcgct ggaagcggca gtaaccttgt atgcagaacc ggaactggcg
     4381 gcgaaactga ccgcgctggg cgatgaatta cgatttgtcc tgttgacctc cggcgctacc
     4441 gttgcagact ataatgacgc acctgctgat gctcagcaga gcgaagtact caaagggctg
     4501 aaagtcgcgt tgagtaaagc cgaaggtgag aagtgcccac gctgctggca ctacacccag
     4561 gatgtcggca aggtggcgga acacgcagaa atctgcggcc gctgtgtcag caacgtcgcc
     4621 ggtgacggtg aaaaacgtaa gtttgcctga tgagtcaatc gatctgttca acagggctac
     4681 gctggctgtg gctggtggta gtcgtgctga ttatcgatct gggcagcaaa tacctgatcc
     4741 tccagaactt tgctctgggg gatcaggtcc cgctgttccc gtcgcttaat ctgcattatg
     4801 cgcgtaacta tggcgcggcg tttagtttcc ttgccgatag cggcggctgg cagcgttggt
     4861 tctttgccgg tattgcgatt ggtattagcg tgatcctggc agtgatgatg tatcgctcga
     4921 aggccacgca gaagctaaac aatatcgctt atgcgctgat tattggcggc gcgctgggca
     4981 acctgttcga ccgcctgtgg cacggcttcg ttgtcgatat gatcgacttc tacgtcggcg
     5041 actggcactt cgccaccttc aaccttgccg atactgccat ttgtgtcggt gcggcactga
     5101 ttgtgctgga aggttttttg ccttctaaag cgaaaaaaca ataataaacc ctgccggata
     5161 aggcctacag attgctgcga aatcgtaggc cgcatccggc aaaaatcctc aaaatataag
     5221 agcaaacctg catgtctgaa tctgtacaga gcaatagcgc cgtcctggtg cacttcacgc
     5281 tgaaactcga tgatggcacc accgctgagt ctacccgcaa caacggtaaa ccggcgctgt
     5341 tccgcctggg cgatgcttct ctttctgaag ggctggagca acacctgttg gggctgaaag
     5401 tgggcgataa aaccaccttc tcgctggagc ccgatgcggc gtttggcgtg ccgtcaccgg
     5461 acctgattca gtacttctcc cgccgtgaat ttatggatgc aggcgagcca gaaattggcg
     5521 caattatgct ttttaccgca atggatggca gtgagatgcc tggcgtgatc cgcgaaatta
     5581 acggcgactc cattaccgtt gatttcaacc atccgctggc cgggcagacc gttcattttg
     5641 atattgaagt gctggaaatc gatccggcac tggaggcgta acatgcagat cctgttggcc
     5701 aacccgcgtg gtttttgtgc cggggtagac cgcgctatca gcattgttga aaacgcgctg
     5761 gccatttacg gcgcaccgat atatgtccgt cacgaagtgg tacataaccg ctatgtggtc
     5821 gatagcttgc gtgagcgtgg ggctatcttt attgagcaga ttagcgaagt accggacggc
     5881 gcgatcctga ttttctccgc acacggtgtt tctcaggcgg tacgtaacga agcaaaaagt
     5941 cgcgatttga cggtatttga cgcgacctgt ccgctggtga ccaaagtgca tatggaagtc
     6001 gcccgcgcca gtcgccgtgg cgaagaatct attctcatcg gtcacgccgg gcacccggaa
     6061 gtggaaggga caatgggcca gtacagtaac ccggaagggg gaatgtatct ggtcgaatcg
     6121 ccggacgatg tgtggaaact gacggtcaaa aacgaagaga agctctcctt tatgacccag
     6181 accacgctgt cggtggatga cacgtctgat gtgatcgacg cgctgcgtaa acgcttcccg
     6241 aaaattgtcg gtccgcgcaa agatgacatc tgctacgcca cgactaaccg tcaggaagcg
     6301 gtacgcgccc tggcagaaca ggcggaagtt gtgttggtgg tcggttcgaa aaactcctcc
     6361 aactccaacc gtctggcgga gctggcccag cgtatgggca aacgcgcgtt tttgattgac
     6421 gatgcgaaag atatccagga agagtgggtg aaagaggtta aatgcgtcgg cgtgactgcg
     6481 ggcgcatcgg ctccggatat tctggtgcag aatgtggtgg cacgtttgca gcagctgggc
     6541 ggtggtgaag ccattccgct ggaaggccgt gaagaaaaca ttgttttcga agtgccgaaa
     6601 gagctgcgtg tcgatattcg tgaagtcgat taagtcatta gcagcctaag ttatgcgaaa
     6661 atgccggtct tgttaccggc attttttatg gagaaaacat gcgtttacct atcttcctcg
     6721 atactgaccc cggcattgac gatgccgtcg ccattgccgc cgcgattttt gcacccgaac
     6781 tcgacctgca actgatgacc accgtcgcgg gtaatgtctc ggttgagaaa actacccgca
     6841 atgccctgca actgctgcat ttctggaatg cggagattcc gctcgcccaa ggggccgctg
     6901 tgccgctggt acgcgcaccg cgtgatgcgg catctgtgca cggcgaatcg ggaatggctg
     6961 gctacgactt tgttgagcac aaccgaaagc cgctcgggat accggcgttt ctggcgattc
     7021 gggatgccct gatgcgtgca ccagagcctg ttaccctggt ggccatcggc ccgttaacca
     7081 atattgcgct gttactttca caatgcccgg aatgcaagcc gtatattcgc cgtctggtga
     7141 tcatgggtgg ttctgccgga cgcggcaact gtacgccaaa cgccgagttt aatattgctg
     7201 ccgatccaga agctgctgcc tgtgtcttcc gcagtggtat tgaaatcgtc atgtgcggtt
     7261 tggatgtcac caatcaggca atattaactc ctgactatct ctctacactg ccgcagttaa
     7321 accgtaccgg gaaaatgctt cacgccctgt ttagccacta ccgtagcggc agtatgcaaa
     7381 gcggcttgcg aatgcacgat ctctgcgcca tcgcctggct ggtgcgcccg gacctgttca
     7441 ctctcaaacc ctgttttgtg gcagtggaaa ctcagggcga atttacctca ggcacgacgg
     7501 tggttgatat cgacggttgc ctgggcaagc cagccaatgt acaggtggca ttggatctgg
     7561 atgtgaaagg cttccagcag tgggtggctg aggtgctggc tctggcgtcg taacctgtca
     7621 catgttattg gcatgcagtc attcatcgac tcatgccttt cactgatatc cctccctgtt
     7681 tatcattaat ttctaattat cagcgttttt ggctggcggc gtagcgatgc gctggttact
     7741 ctgaaaacgg tctatgcaaa ttaacaaaag agaatagcta tgcatgatgc aaacatccgc
     7801 gttgccatcg cgggagccgg ggggcgtatg ggccgccagt tgattcaggc ggcgctggca
     7861 ttagaaggcg tgcagctggg cgcggcgctg gagcgcgaag gatcttcttt actgggcagc
     7921 gacgccggtg agctggctgg tgcagggaaa acaggcgtta ccgtgcaaag cagcctcgat
     7981 gcggtaaaag atgattttga tgtgtttatc gattttaccc gtccggaagg tacgctgaac
     8041 catctcgctt tttgtcgcca gcatggcaaa gggatggtga tcggcactac ggggtttgac
     8101 gaagccggta aacaggcgat tcgtgacgcc gccgccgata ttgcgattgt ctttgccgcc
     8161 aattttagcg ttggcgttaa cgtcatgctt aagctgctgg agaaagcagc caaagtgatg
     8221 ggtgactaca ccgatatcga aattattgaa gcacatcata gacataaagt tgatgcgccg
     8281 tcaggcaccg cactggcaat gggagaggcg atcgcccacg cccttgataa agatctgaaa
     8341 gattgcgcgg tctacagtcg tgaaggccac accggtgaac gtgtgcctgg caccattggt
     8401 tttgccaccg tgcgtgcagg tgacatcgtt ggcgaacata ccgcgatgtt tgccgatatt
     8461 ggcgagcgtc tggagatcac ccataaggcg tccagccgca tgacatttgc taacggcgcg
     8521 gtaagatcgg ctttgtggtt gagtggtaag gaaagtggtc tttttgatat gcgagatgtg
     8581 cttgatctca atagtttgta
//
LOCUS       DAZKHF020000188         8427 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2201S31241
            SAMN43267800-rid25106413.denovo.189, whole genome shotgun sequence.
ACCESSION   DAZKHF020000188 DAZKHF020000000
VERSION     DAZKHF020000188.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267800
            Sequence Read Archive: SRR30307263
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 8427)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8427)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 8427)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350581.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350581.2
            Genome Coverage       :: 151x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:21:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,652
            CDSs (total)                      :: 5,557
            Genes (coding)                    :: 5,320
            CDSs (with protein)               :: 5,320
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 237
            CDSs (without protein)            :: 237
            Pseudo Genes (ambiguous residues) :: 0 of 237
            Pseudo Genes (frameshifted)       :: 102 of 237
            Pseudo Genes (incomplete)         :: 130 of 237
            Pseudo Genes (internal stop)      :: 52 of 237
            Pseudo Genes (multiple problems)  :: 41 of 237
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8427
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267800-rid25106413.denovo.189"
                     /serotype="O 26:H11"
                     /isolate="2201S31241"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-01"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            119..2497
                     /gene="ppsA"
                     /locus_tag="ACHOSC_RS17620"
                     /old_locus_tag="pgaptmp_003524"
     CDS             119..2497
                     /gene="ppsA"
                     /locus_tag="ACHOSC_RS17620"
                     /old_locus_tag="pgaptmp_003524"
                     /EC_number="2.7.9.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008986 - pyruvate, water dikinase
                     activity [Evidence IEA];
                     GO_function: GO:0016772 - transferase activity,
                     transferring phosphorus-containing groups [Evidence IEA];
                     GO_process: GO:0006090 - pyruvate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate synthase"
                     /protein_id="HGU4549402.1"
                     /translation="MSNNGSSPLVLWYNQLGMNDVDRVGGKNASLGEMITNLSGMGVS
                     VPNGFATTADAFNQFLDQSGVNQRIYELLDKTDIDDVTQLAKAGAQIRQWIIDTPFQP
                     ELENAIREAYAQLSADDENASFAVRSSATAEDMPDASFAGQQETFLNVQGFDAVLVAV
                     KHVFASLFNDRAISYRVHQGYDHRGVALSAGVQRMVRSDLASSGVMFSIDTESGFDQV
                     VFITSAWGLGEMVVQGAVNPDEFYVHKPTLAANRPAIVRRTMGSKKIRMVYAPTQEHG
                     KQVKIEDVPQEQRDIFSLTNEEVQELAKQAVQIEKHYGRPMDIEWAKDGHTGKLFIVQ
                     ARPETVRSRGQVMERYTLHSQGKIIAEGRAIGHRIGAGPVKVIHDISEMNRIEPGDVL
                     VTDMTDPDWEPIMKKASAIVTNRGGRTCHAAIIARELGIPAVVGCGDATERMKDGENV
                     TVSCAEGDTGYVYAELLEFSVKSSSVETMPDLPLKVMMNVGNPDRAFDFACLPNEGVG
                     LARLEFIINRMIGVHPRALLEFDDQEPQLQNEIREMMKGFDSPREFYVGRLTEGIATL
                     GAAFYPKRVIVRLSDFKSNEYANLVGGERYEPDEENPMLGFRGAGRYVSDSFRDCFAL
                     ECEAVKRVRNDMGLTNVEIMIPFVRTVDQAKAVVEELARQGLKRGENGLKIIMMCEIP
                     SNALLAEQFLEYFDGFSIGSNDMTQLALGLDRDSGVVSELFDERNDAVKALLSMAIRA
                     AKKQGKYVGICGQGPSDHEDFAAWLMEEGIDSLSLNPDTVVQTWLSLAELKK"
     gene            complement(2554..4200)
                     /gene="fadK"
                     /locus_tag="ACHOSC_RS17625"
                     /old_locus_tag="pgaptmp_003525"
     CDS             complement(2554..4200)
                     /gene="fadK"
                     /locus_tag="ACHOSC_RS17625"
                     /old_locus_tag="pgaptmp_003525"
                     /EC_number="6.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310435.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="medium-chain fatty-acid--CoA ligase"
                     /protein_id="HGU4549403.1"
                     /translation="MKVTLTFNEQRRAAYRQQGLWGDASLADYWQQTARAMPDKIAVV
                     DNHGASYTYSALDHAASCLANWMLAKGIESGDRIAFQLPGWCEFTVIYLACLKIGAVS
                     VPLLPSWREAELVWVLNKCQAKMFFAPTLFKQTRPVDLILPLQNQLPQLQQIVGVDKL
                     APATSSLSLSQIIADNTPLTTAITTHGDELAAVLFTSGTEGLPKGVMLTHNNILASER
                     AYCARLNLTWQDVFMMPAPLGHATGFLHGVTAPFLIGARSVLLDIFTPDACLALLEQQ
                     RCTCMLGATPFVYDLLNVLEKQPADLSALRFFLCGGTTIPKKVARECQQLGIKLLSVY
                     GSTESSPHAVVNLDDPLSRFMHTDGYAAAGVEIKVVDDARKTLPPGCEGEEASRGPNV
                     FMGYFDEPELTARALDEEGWYYSGDLCRMDEAGYIKITGRKKDIIVRGGENISSREVE
                     DILLQHPKIHDACVVAMPDERLGERSCAYVVLKAPHHSLSLEEVVAFFSRKRVAKYKY
                     PEHIVVIEKLPRTASGKIQKFLLRKDIMRRLTQDVCEEIE"
     gene            complement(4257..4550)
                     /locus_tag="ACHOSC_RS17630"
                     /old_locus_tag="pgaptmp_003526"
     CDS             complement(4257..4550)
                     /locus_tag="ACHOSC_RS17630"
                     /old_locus_tag="pgaptmp_003526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin family protein"
                     /protein_id="HGU4549404.1"
                     /translation="MSQNATVNVDIKLGVNKFHVDEGHPHIILAANPDINEFRKLMKA
                     CPAGLYKQDDAGNIHFDSAGCLECGTCRVLCGNTILEQWQYPAGTFGIDFRYG"
     gene            complement(4547..5836)
                     /locus_tag="ACHOSC_RS17635"
                     /old_locus_tag="pgaptmp_003527"
     CDS             complement(4547..5836)
                     /locus_tag="ACHOSC_RS17635"
                     /old_locus_tag="pgaptmp_003527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HGU4549405.1"
                     /translation="MSEDKFDAIVVGAGVAGSVAALVMARAGLDVLVIERGDSAGCKN
                     MTGGRLYAHTLEAIIPGFAVSAPVERKVTREKISFLTEESAVTLDFHREQPDVPQHAS
                     YTVLRNRLDPWLMEQAEQAGAQFIPGVRVDALVREGNKVTGVQAGDDILEANVVILAD
                     GVNSMLGRSLGMVPASDPHHYAVGVKEVIGLTPEQINDRFNITGEEGAAWLFAGSPSD
                     GLMGGGFLYTNKDSISLGLVCGLGDIAHAQKSVPQMLEDFKQHPAIRPLISGGKLLEY
                     SAHMVPEGGLAMVPQMVNDGVMIVGDAAGFCLNLGFTVRGMDLAIASAQAAATTVIAA
                     KEREDFSASSLAQYKRELEQSCIMRDMQHFRKIPALMENPRLFSQYPRMVADIMNEMF
                     TIDGKPNQPVRKMIMGHAKKIGLINLLKDGIKGATAL"
     gene            complement(5892..6830)
                     /locus_tag="ACHOSC_RS17640"
                     /old_locus_tag="pgaptmp_003528"
     CDS             complement(5892..6830)
                     /locus_tag="ACHOSC_RS17640"
                     /old_locus_tag="pgaptmp_003528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit alpha"
                     /protein_id="HGU4549406.1"
                     /translation="MSQLNSVWVFSDNPERYAELFGGAQQWGQQVYAIVQNTDQAQAV
                     MPYGPKCIYVLEQNDALQRTENYAESIAALLKDKHPAMLLLAATKRGKALAARLSVQL
                     NAALVNDATAVDIVDGHICAEHRMYGGLAFAQEKINSPLAIITLAPGVQEPCTSDTSH
                     QCPTETVPYVAPRHEILCRERRAKAASSVDLSKAKRVVGVGRGLAAQDDLKMVHELAA
                     VLNAEVGCSRPIAEGENWMERERYIGVSGVLLKSDLYLTLGISGQIQHMVGGNGAKVI
                     AAINKDKNAPIFNYADYGLVGDIYKVVPALISQLSR"
     gene            complement(6850..7614)
                     /locus_tag="ACHOSC_RS17645"
                     /old_locus_tag="pgaptmp_003529"
     CDS             complement(6850..7614)
                     /locus_tag="ACHOSC_RS17645"
                     /old_locus_tag="pgaptmp_003529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein"
                     /protein_id="HGU4549407.1"
                     /translation="MKIITCFKLVPEEQDIVVTPEYTLNFDNADAKISQFDLNAIEAA
                     SQLATDDDEIAALTVGGSLLQNSKVRKDVLSRGPHSLYLVQDAQLEHALPLDTAKALA
                     AAIEKIGFDLLIFGEGSGDLYAQQVGLLVGEILQLPVINAVSAIQRQGNTLVIERTLE
                     DDVEVIELSVPAVLCVTSDINVPRIPSMKAILGAGKKPVNQWQASDIDWSQSAPLAEL
                     VGIRVPPQTERKHIIIDNDSPEAIAELAEHLKKALN"
     gene            7930..>8427
                     /locus_tag="ACHOSC_RS17650"
                     /old_locus_tag="pgaptmp_003530"
     CDS             7930..>8427
                     /locus_tag="ACHOSC_RS17650"
                     /old_locus_tag="pgaptmp_003530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HGU4549408.1"
                     /translation="MYQRCFDNASETLFVAGKTPRLSRFAFSDDPKWESGHHVHDNET
                     ELIYVKKGVARFTIDSSLYVAHADDIVVIERGRLHAVASDVNDPATTCTCALYGFQFQ
                     GAEENQLLQPHSCPVIAAGQGKEVIKTLFNELSVILPQSKNSQTSSLWDAFAYTLAIL
                     YYENFK"
BASE COUNT         2010 a         2180 c         2180 g         2057 t
ORIGIN      
        1 gtaaaaaaat atatttgctt gaacgattca ccgttttttt catccggtta aatatgcaaa
       61 gataaatgcg cagaaatgtg tttctcaaac cgttcattta tcacaaaagg attgttcgat
      121 gtccaacaat ggctcgtcac cgctggtgct ttggtataac caactcggca tgaatgatgt
      181 agacagggtt gggggcaaaa atgcctccct gggtgaaatg attactaacc tttccggaat
      241 gggtgtttcc gttccgaatg gtttcgccac aaccgccgac gcgtttaacc agtttctgga
      301 ccaaagcggc gtaaaccagc gcatttatga actgctggat aaaacggata ttgacgatgt
      361 tacccagctt gcgaaagcgg gcgcgcaaat ccgccagtgg attatcgaca ctcccttcca
      421 gcctgagctg gaaaatgcca tccgcgaagc ttatgcacag ctttccgccg atgacgaaaa
      481 cgcctctttt gcggtgcgct cctccgctac tgcagaagat atgccggacg cttcctttgc
      541 cggtcagcag gaaaccttcc tcaacgttca gggttttgac gccgttctcg tggcagtgaa
      601 acatgtattt gcttctctgt ttaacgatcg cgccatctct tatcgtgtgc accagggtta
      661 cgatcaccgt ggcgtggcgc tctccgccgg tgttcaacgg atggtgcgct ctgacctcgc
      721 atcttctggc gtgatgttct ccattgatac cgaatccggc tttgaccagg tggtgtttat
      781 cacttccgca tggggccttg gtgaaatggt cgtgcagggc gcggttaacc cggatgagtt
      841 ttacgtccat aaaccgacac ttgcagcgaa tcgcccggct atcgtgcgtc gcaccatggg
      901 gtcgaaaaaa atccgcatgg tttacgcgcc gacccaggag cacggcaagc aggttaaaat
      961 cgaagacgta ccgcaggaac agcgtgacat cttctcgctg accaacgaag aagtgcagga
     1021 actggcaaaa caggccgtac aaattgagaa acactacggt cgcccgatgg atattgagtg
     1081 ggcgaaagat ggccacaccg gcaaactgtt cattgtgcag gcgcgtccgg aaaccgtgcg
     1141 ctcacgcggt caggtcatgg agcgttatac gctgcattca cagggtaaga ttatcgccga
     1201 aggccgtgct atcggtcatc gcatcggcgc gggtccggtg aaagtcatcc atgacatcag
     1261 cgaaatgaac cgcatcgaac ctggcgacgt gctggtcact gacatgaccg acccggactg
     1321 ggaaccgatc atgaagaaag catctgccat cgtcaccaac cgtggcggtc gtacctgtca
     1381 cgcggcgatc atcgctcgtg aactgggcat tccggcggta gtgggctgtg gtgatgcaac
     1441 agaacggatg aaagacggtg agaacgtcac tgtttcttgt gccgaaggtg ataccggtta
     1501 cgtctatgcg gagttgctgg aatttagcgt gaaaagctcc agcgtagaaa cgatgccgga
     1561 tctgccgttg aaagtgatga tgaacgtcgg taacccggac cgtgctttcg acttcgcctg
     1621 cctgccgaac gaaggcgtgg gccttgcgcg tctggaattt atcatcaacc gtatgattgg
     1681 cgtccaccca cgcgcactgc ttgagtttga cgatcaggaa ccgcagttgc aaaacgaaat
     1741 ccgcgagatg atgaaaggtt ttgattctcc gcgtgaattt tacgttggtc gtctgactga
     1801 agggatcgcg acgctgggtg ccgcgtttta tccgaagcgc gtcattgtcc gtctctctga
     1861 ttttaaatcg aacgaatatg ccaacctggt cggtggtgag cgttacgagc cagatgaaga
     1921 gaacccgatg ctcggcttcc gtggcgcggg ccgctatgtt tccgacagct tccgcgactg
     1981 cttcgcgctg gagtgtgaag cagtgaaacg agtgcgcaac gacatggggc tgaccaacgt
     2041 tgagatcatg atcccgttcg tgcgtaccgt agatcaggcg aaagcggtgg ttgaagaact
     2101 ggcgcgtcag gggctgaaac gtggcgagaa cgggctgaaa atcatcatga tgtgtgaaat
     2161 tccgtccaac gccttgctgg cagagcagtt cctcgaatat ttcgacggct tctcaattgg
     2221 ctcaaacgat atgacacagc tggcgctcgg tctggatcgt gactccggcg tggtgtctga
     2281 attgttcgat gagcgcaacg atgcggtgaa agcattgctg tcgatggcta tccgtgccgc
     2341 gaagaaacag ggcaaatatg tcgggatttg cggtcagggt ccgtccgacc atgaagactt
     2401 tgccgcatgg ttgatggaag aggggatcga tagcctgtct ctgaacccgg acaccgtggt
     2461 gcaaacctgg ttaagcctgg ctgaactgaa gaaataaaat aaatccccgg cggcgttcag
     2521 tcgccgggtt tatgtgatcc ccgaagataa aacttattca atctcttcac agacatcctg
     2581 cgttaatcgc cgcataatat cttttcttaa caaaaacttt tgtattttac ctgaggcagt
     2641 gcgcggtagt ttttcgatta ccacgatatg ttcaggatat ttatattttg cgacccgttt
     2701 acggctaaaa aaagccacca cctcttccag cgataatgaa tgatgcggcg ctttcagcac
     2761 gacataagcg catgatcgtt cacctaaacg ttcatcgggc attgcaacca cacaggcatc
     2821 gtgaatttta ggatgctgca ataaaatatc ttccacttca cggctgctaa tattttcgcc
     2881 gccgcggaca ataatatctt ttttgcgtcc ggtaattttt atatagccag cctcatccat
     2941 acggcagaga tcgccgctgt aataccagcc ttcttcatcc agcgcacggg cggttaattc
     3001 aggttcatca aaatatccca taaacacatt ggggccacgc gaggcttctt caccttcgca
     3061 acctggcggt aaggtcttgc gtgcgtcatc gaccacttta atctctacac ctgcggcagc
     3121 gtaaccatcg gtgtgcataa agcgcgacaa aggatcatcg agattcacca ccgcatgcgg
     3181 cgaactttct gtggaaccat aaacacttaa taatttaatg ccgagctgct ggcattcacg
     3241 cgccactttt ttggggattg tggttccgcc gcaaagaaag aaacgcagcg ctgacaggtc
     3301 cgcgggttgt ttctctagta cattcaaaag atcatagaca aacggcgttg cgcctagcat
     3361 acaggtgcaa cgctgctgct caagcagcgc gagacacgca tcaggagtga aaatatctaa
     3421 caacacgctg cgagcgccaa ttaagaatgg tgccgttacg ccatgcagaa agcccgttgc
     3481 gtggccgagt ggcgcaggca tcataaagac atcctgccag gtcagattca gccgcgcgca
     3541 ataagcccgc tcactggcga gaatattgtt atgcgttagc atcacgccct ttggcagacc
     3601 ctcggttccg gaggtaaaca gcaccgcagc taattcatcg ccgtgggtcg ttatcgccgt
     3661 ggtcagtggg gtattgtcgg cgataatctg acttaatgag agggaagagg tggcgggagc
     3721 cagtttgtcc acgccgacaa tttgttgtag ttgtggaagc tgattttgca gcggcaggat
     3781 taaatctacc gggcgcgttt gtttaaacaa cgtcggtgca aagaacattt ttgcctgaca
     3841 cttattgagc acccacacca gttctgcttc ccgccaggaa ggcaacagcg gcacggaaac
     3901 cgcaccgatt ttcaggcagg caagatagat aacggtaaat tcacaccagc caggcagttg
     3961 aaatgcgatg cgatcgcctg attcaatacc cttcgctaac atccagtttg ccagacagct
     4021 cgcggcgtga tcgagcgcgc tataggtgta cgatgcacca tgattatcga ccacggcaat
     4081 tttgtctggc atcgcacgag cggtctgctg ccagtaatcg gccagcgaag catcgcccca
     4141 tagcccttgc tgacgatacg ccgcacgacg ttgttcgttg aacgttaatg tcactttcat
     4201 gttggactct cgaaacagaa catcaggccc gagatgcggg cctgtgggat gcatgattag
     4261 ccgtagcgaa aatcaatacc gaaggtgcct gcgggatatt gccactgttc gagaatagtg
     4321 ttaccgcaca gcacccgaca ggtgccgcac tccagacaac cggcagaatc aaaatgaatg
     4381 ttgccggcgt catcctgctt ataaagtccg gcagggcagg ctttcattaa tttacggaat
     4441 tcattgatat cgggatttgc cgccagaatg atatgcgggt ggccctcatc aacatggaat
     4501 ttattgacgc ctaatttgat gtcgacgtta accgtagcgt tctggctcat agcgcggttg
     4561 ctcccttaat gccatctttc agcaagttga tcagcccaat tttcttcgca tgtcccatga
     4621 tcattttgcg taccggctgg tttggtttgc cgtcaatggt gaacatctcg ttcatgatgt
     4681 cggcgaccat tcgtgggtat tggctaaaca ggcgcgggtt ttccatcagc gccgggatct
     4741 tgcggaaatg ctgcatatcg cgcatgatgc agctttgttc cagctcacgt ttgtattgcg
     4801 ccagactgct ggcggagaaa tcctcgcgtt ctttggcggc gatcaccgtt gtggcggcag
     4861 cctgagccga tgcaatggct aaatccatgc cacgaactgt aaaacccaaa ttcaggcaga
     4921 agcctgcggc gtcaccaacg atcatcacgc catcgttaac catctgcggc accattgcca
     4981 gaccgccttc tggcaccata tgcgcggaat attcaagcag tttgccgccg ctaattagtg
     5041 ggcgaatggc ggggtgttgt ttaaaatctt ccagcatttg cggcacgctt ttttgcgcat
     5101 gggcgatatc acccaatcca caaaccagcc ccaaggatat ggaatccttg ttggtataga
     5161 gaaatccccc gcccatcagg ccgtcagaag gggaaccggc aaacagccag gcggcacctt
     5221 cctcgcccgt aatattaaag cgatcgttga tctgttctgg tgtgaggcca ataacctctt
     5281 taacaccaac agcgtaatga tgcggatcgg aagcgggaac cattcccagc gagcggccaa
     5341 gcatcgagtt aacgccatca gccagaatca ccacattcgc ttcgagaata tcatccccag
     5401 cctgcacgcc agtgaccttg tttccttcac gaaccagcgc atcgacgcga acgcctggga
     5461 taaactgcgc gcccgcctgc tcggcttgtt ccatcaacca cgggtccaga cgattacgca
     5521 atacggtata agatgcgtgt tgcggaacat ctggttgctc gcggtgaaaa tcgagggtaa
     5581 cggcgctttc ttcggttaag aaggagattt tctcgcgtgt gaccttgcgt tctaccggcg
     5641 ctgatactgc aaagcctgga atgattgctt caagtgtgtg ggcataaaga cgcccgccgg
     5701 tcatgttttt acatccggca ctgtcgccgc gttctatcac caggacatcc agcccggctc
     5761 gcgccatgac cagtgcggca acgctaccag caacgcccgc accgaccaca atggcatcaa
     5821 atttgtcttc cgacatgcgt tatgctccct tcccgctgcg tttacagcgg gagatgtggt
     5881 tgaaagggga attagcggct caactggcta atcagggcag ggacgacttt gtagatatcg
     5941 cccaccagac cgtagtcggc ataattgaag attggcgcat ttttatcttt attgatggcg
     6001 gcaatcactt ttgcgccgtt gccgccaacc atatgctgga tctgcccgga gatccccagc
     6061 gtcaggtaga gatcggattt cagcaacacg ccggagacac cgatataacg ttcacgctcc
     6121 atccagttct cgccttcggc aattggacgt gaacagccga cttcagcatt cagcaccgcc
     6181 gccagttcgt ggaccatttt caggtcatcc tgcgccgcca gaccacgacc gacgccaacc
     6241 acacgttttg ctttgctcag gtccacgctg cttgcggctt tcgcacggcg ttcgcgacag
     6301 agaatttcat gacgcggagc aacataaggt accgtttctg tcgggcactg atgagaggta
     6361 tcactggtgc acggttcctg aacaccgggt gcaagggtaa tgatcgccag cgggctgttg
     6421 atcttttcct gagcgaacgc taacccgcca tacatccggt gttcggcgca aatgtgacca
     6481 tcgacaatat ccaccgccgt ggcatcgttc accagcgccg cattcagttg cacacttaac
     6541 cgtgctgcca gagctttacc acgtttcgtc gcggccaaca gcaacatagc ggggtgttta
     6601 tctttcagca gggcagcaat gctttcggcg taattttcag tgcgttgcag cgcgtcgttt
     6661 tgctcaagaa cataaataca ttttggacca taaggcataa ctgcctgcgc ctggtcggta
     6721 ttttgtacaa tggcatacac ctgttggccc cattgctgag cgccgccaaa cagttcagca
     6781 taacgttcag gattatcgct aaagacccag acgctgttta attgactcat cgtttcttct
     6841 ccgtaggctt cagttcagag ctttcttcag atgttccgcc agctcggcaa tggcctccgg
     6901 cgaatcgtta tcgataatga tgtgcttacg ttctgtttgc ggcggtacgc gaatgccgac
     6961 cagttcagca agtggcgcgc tctggctcca gtcaatatca cttgcctgcc actgatttac
     7021 cggtttttta cccgcgccga gaatggcttt catcgaagga atgcgtggca cgttaatatc
     7081 ggaggtgacg cagagcacgg ctggaacaga gagttcaata acttcaacat catcttcaag
     7141 cgtgcgttca atcaccagtg tattgccctg acgctgaata gcactcactg cattaatcac
     7201 cggaagttgc agaatttctc cgaccagcaa gccaacctgc tgggcataaa ggtcgccgga
     7261 accttcacca aagatcagta aatcgaagcc gatcttttca attgctgccg ccagcgcttt
     7321 tgcggtatcg agaggcagtg catgttcaag ttgcgcatcc tgcaccaaat acaggctgtg
     7381 cggcccgcgg gatagcacgt ctttgcgcac tttcgagttc tgcaacaatg agccgccaac
     7441 ggtcagcgcg gctatctcat cgtcatctgt tgcgagctgg cttgcagctt caatggcatt
     7501 gagatcgaac tggctgattt tggcgtcggc attgtcgaaa ttcagggtgt attctggagt
     7561 gacaacaatg tcctgttctt caggcaccag cttaaagcag gttattattt tcatgatttc
     7621 tcctgtgagt cgcatacgga accatctccg atggaaggta aaaatggcct gaaaatagtg
     7681 tgcgatagcg gtgcggggtt ttcgacggag caagctgttt tccttgccaa caatcttgca
     7741 aaaaaaatgc gatcgctaaa acaaaagtgc aatttgcggc aatatagtta ttccatgacc
     7801 gtacaatctt gcattcagtg taaataatct tgtttagctc tcgctaaacg cggttgtata
     7861 accgcaatac acgccgtatt ttatccggca tatattgttt ttgcttgctc atggaatagg
     7921 gaggttgtca tgtatcaacg ctgttttgat aacgccagtg aaacgctgtt tgtcgccggt
     7981 aaaacgccac ggctttcacg ttttgcattt agcgatgatc caaaatggga gtccggacat
     8041 cacgttcatg acaatgaaac cgagttgatt tacgtcaaga aaggggttgc aaggtttacc
     8101 atcgattctt cgttatatgt cgcacatgcg gatgacattg tggtgataga acgcggcagg
     8161 ctacatgcgg tggcctctga cgttaacgat ccggcaacga cgtgtacctg tgcgctgtac
     8221 ggctttcagt ttcagggggc tgaggaaaat cagctactgc aaccgcattc ttgtccggta
     8281 attgccgcgg ggcagggaaa agaagtcata aaaaccttat ttaatgagct aagtgtgatt
     8341 ttgccgcaaa gtaaaaatag ccaaacatct tcgttatggg atgcatttgc ctatacgtta
     8401 gccattcttt actacgaaaa ttttaaa
//
LOCUS       DAZKHF020000189         8425 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2201S31241
            SAMN43267800-rid25106413.denovo.190, whole genome shotgun sequence.
ACCESSION   DAZKHF020000189 DAZKHF020000000
VERSION     DAZKHF020000189.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267800
            Sequence Read Archive: SRR30307263
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 8425)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8425)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 8425)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350581.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350581.2
            Genome Coverage       :: 151x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:21:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,652
            CDSs (total)                      :: 5,557
            Genes (coding)                    :: 5,320
            CDSs (with protein)               :: 5,320
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 237
            CDSs (without protein)            :: 237
            Pseudo Genes (ambiguous residues) :: 0 of 237
            Pseudo Genes (frameshifted)       :: 102 of 237
            Pseudo Genes (incomplete)         :: 130 of 237
            Pseudo Genes (internal stop)      :: 52 of 237
            Pseudo Genes (multiple problems)  :: 41 of 237
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8425
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267800-rid25106413.denovo.190"
                     /serotype="O 26:H11"
                     /isolate="2201S31241"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-01"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            <1..2582
                     /gene="cas3"
                     /locus_tag="ACHOSC_RS17655"
                     /old_locus_tag="pgaptmp_003531"
     CDS             <1..2582
                     /gene="cas3"
                     /locus_tag="ACHOSC_RS17655"
                     /old_locus_tag="pgaptmp_003531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001233973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="CRISPR-associated helicase Cas3'"
                     /protein_id="HGU4549409.1"
                     /translation="HSLDVAAMGYLMVKRNCFGLADYFRQLGISDKEQAAQFFAWLLC
                     WHDIGKFARSFQQLYLPPELKIQEGARKNYEKISHSTLGYWLWNHYLSECQELLPSSS
                     LSPRKLRRVIEMWMPVTTGHHGQPPDRMDELDNFLPEDKAAARDFLLEIKPLFPLIEI
                     PAFWDDDEGIELIKHLSWYISATVVLADWTGSSTRFFPRVAHPMDIKGYWQKTLIQAQ
                     NALTVFPLKAKVAPFNGINTLFPFIENPTPLQQKVLDLDISQQGPQLFILEDVTGAGK
                     TEAALILAHRLIAAGKAQGLFFGLPTMATANAMYDRLVKTWLAFYSPESRPSLVLAHS
                     ARTLMDRFNESLWSGDLVGSEEPDEQTFSQGCAAWFADSNKKALLAEIGVGTLDQAMM
                     AVMPFKHNNLRLLGLSNKILLADEIHACDAYMSCILEGLIERQARGGNSVILLSATLS
                     QQQRDKLVAAFARGTEGQQEAPFLEKDDYPWLTHVTKSDVNSHRVATRKDVERSVSVG
                     WLHSEQECIARIESAVSQGKCIAWIRNSVDDAIKVHRQLLARGVIPASSLSLFHSRFA
                     FSDRQRIETETLARFGKYCSLQRASQVIVCTQVIEQSVDIDLDEMISDLAPVDLLIQR
                     AGRLQRHIRDINGQLKRDGKDERSPPELLILAPVWDDSPGDEWFGSAMRNSAFVYPDH
                     GRIWLTQRVLREQGAIQMPHAARLLIESVYGEDVVMPEGFARSEQEQVGKYYCDRAMA
                     KKFVLNFRPGYAANINDYLPEKLSTRLAEESVSLWLATCIDGVVKPYATGAHAWEMSV
                     VRVRRSWWKKHRDEFSLLEGEAFRLWCIEQRQDPEMANVILVNDDESCGYSATEGLIG
                     KVG"
     gene            2680..4242
                     /gene="casA"
                     /locus_tag="ACHOSC_RS17660"
                     /old_locus_tag="pgaptmp_003532"
                     /gene_synonym="cse1"
     CDS             2680..4242
                     /gene="casA"
                     /locus_tag="ACHOSC_RS17660"
                     /old_locus_tag="pgaptmp_003532"
                     /gene_synonym="cse1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I-E CRISPR-associated protein Cse1/CasA"
                     /protein_id="HGU4549410.1"
                     /translation="MNSFSLLTTPWLPVRFKDGTTGKLAPVDLADENVVDIAAPRADL
                     QGAAWQFLLGLLQTSFAPKNHGRWDDIWQDGLEAEKLREALLSLEHAFQFGADSPSFM
                     QDFEALKGDKVQVASLLPEIPGAQTTKFNKDHFIKRGVTEHVCPHCSALALFSLQLNA
                     PSGGKGYRTGLRGGGPMTTLIELQEYQGNQQTPLWRKLWPNVMPQDEADLPLPKKFDD
                     LVFPWLGPTRTSELAGAVVTHDQVNKLQAYWGMPRRIRIDFNTTTVGNCDICGEQSDA
                     LLSLMTTKNYGANYAMWQHPLTPYRIPLKEGGEFYSVKPQPGGLIWRDWLGLIETGKS
                     ENNTELPALVVKLFNASSLKQAKVGLWGFGYDFDNMKARCWYEHHFPLLLKKKEGQIP
                     KLRLAAQTASRILSLLRSALKEAWFSDPKGARGDFSFVDIDFWNKTQHRFLRLVRQIE
                     EGQDADELLGKWQKEIWLFARQDFDERVFTNPYEPVDLKRVMTARKKYFTTSAEKQSA
                     KAAREKKQEAAE"
     gene            4239..4727
                     /gene="casB"
                     /locus_tag="ACHOSC_RS17665"
                     /old_locus_tag="pgaptmp_003533"
                     /gene_synonym="cse2"
     CDS             4239..4727
                     /gene="casB"
                     /locus_tag="ACHOSC_RS17665"
                     /old_locus_tag="pgaptmp_003533"
                     /gene_synonym="cse2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000029341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I-E CRISPR-associated protein Cse2/CasB"
                     /protein_id="HGU4549411.1"
                     /translation="MSIVKEEHKATLRKWREELQAKRGNRASLRRSTTVNDVCLSEGF
                     RSLLMQTHTLWKIEAQEWRFTALALVAAVSANVKAIDERQPFAAQLAAVMSKGRFTRL
                     SAVKTPDELLRQLRRAVRLLNGSVNLDSLAEGVFRWCQESDDLLNHHRRQQRPTEFIR
                     IR"
     gene            4790..5845
                     /gene="cas7e"
                     /locus_tag="ACHOSC_RS17670"
                     /old_locus_tag="pgaptmp_003534"
     CDS             4790..5845
                     /gene="cas7e"
                     /locus_tag="ACHOSC_RS17670"
                     /old_locus_tag="pgaptmp_003534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I-E CRISPR-associated protein
                     Cas7/Cse4/CasC"
                     /protein_id="HGU4549412.1"
                     /translation="MTTFIQLHLLTAYPAANLNRDDTGAPKTVVLGGATRLRVSSQSL
                     KRAWRTSALFEQALAGHIGIRSGRIAREAATILIEKGIEDKKAIEWSAKIADYLGKAK
                     NDKKPKDPLTNAETEQLVHISPAEFDAVKALAHQLAEEKRAPKEEDLALLRKDRMAVD
                     IAMFGRMLANKPEFNVEAACQVAHAFGVSETIVEDDFFTAVDDLRQASEDAGAGHLGE
                     TGFGSALFYTYICIDKDLLVENLGGDEALANQTIRAFTEAALKVSPTGKQNSFASRAY
                     ASWALAEKGTDQPRSLAAAFYEPINGTRQLDVAVQRITTLRENMNTVYEQKTECASFD
                     VMNKQGSMKDVLDFICA"
     gene            5856..6602
                     /gene="cas5e"
                     /locus_tag="ACHOSC_RS17675"
                     /old_locus_tag="pgaptmp_003535"
     CDS             5856..6602
                     /gene="cas5e"
                     /locus_tag="ACHOSC_RS17675"
                     /old_locus_tag="pgaptmp_003535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA];
                     GO_process: GO:0051607 - defense response to virus
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I-E CRISPR-associated protein Cas5/CasD"
                     /protein_id="HGU4549413.1"
                     /translation="MSQYLIFQLHGPMASWGGDAPGEVRHTHELPSRSALLGLLAAGV
                     GIRRDDTERLNAFNRHYSLVVCASRNPRWARDYHTVQMPKEVRKARYFSRREELSDPE
                     LLSAIISRRDYYTDAWWMVAVATTADAPYSLEQLQDGLRHPVFPLYLGRKSHPLALPL
                     APLLLEGNACDALCNAYQQYQDHFHKLKVSLPKLQDECWWEGKHDGLVASKILRRRDV
                     PLNRQQWLFGERTVNQGPWLSKEEPCTSQE"
     gene            6584..7234
                     /gene="cas6e"
                     /locus_tag="ACHOSC_RS17680"
                     /old_locus_tag="pgaptmp_003536"
     CDS             6584..7234
                     /gene="cas6e"
                     /locus_tag="ACHOSC_RS17680"
                     /old_locus_tag="pgaptmp_003536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000281458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I-E CRISPR-associated protein
                     Cas6/Cse3/CasE"
                     /protein_id="HGU4549414.1"
                     /translation="MYLSRITLHTGQLSPAQLLHLVDRGEYVMHQWLWDLFPGGKERQ
                     FLYRREELQGAFRFFVLSQERPAESDTFTIECRSFAPELCTGQQLCFNLRANPTICKA
                     GKRHDLLMEAKRQVRGPAEGSDVWLHQQQAALDWLAAQGERSGFTLLDTSVDAYRQQQ
                     LRRENSRQLIQFSSVDYTGMLTVTDPGLFVQRLSQGYGKSRAFGCGLMLIKPGAEA"
     gene            7231..8151
                     /gene="cas1e"
                     /locus_tag="ACHOSC_RS17685"
                     /old_locus_tag="pgaptmp_003537"
     CDS             7231..8151
                     /gene="cas1e"
                     /locus_tag="ACHOSC_RS17685"
                     /old_locus_tag="pgaptmp_003537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_461859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004520 - DNA endonuclease activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA];
                     GO_process: GO:0051607 - defense response to virus
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I-E CRISPR-associated endonuclease Cas1e"
                     /protein_id="HGU4549415.1"
                     /translation="MTFVPLSPIPLKDRTSMIFLQYGQIDVLDGAFVLIDKTGIRTHI
                     PVGSVACIMLEPGTRVSHAAVHLAATVGTLLVWVGEAGVRVYSSGQPGGARADKLLYQ
                     AKLALTEDLRLKVVRKMYELRFREPQPARRSVEQLRGIEGSRVRQTYALLAKQYGVKW
                     NGRKYDPKDWEKGDVVNRCISAATSCLYGISEAAVLAAGYAPAIGFIHSGKPLSFVYD
                     IADIIKFDSVVPKAFEIAARQPAEPDKEVRLACRDIFRSTKLTGKLIPLIEEVLAAGE
                     IEPPQPAPDMLPPAIPEPETLGDSGHRGRG"
     gene            8151..>8425
                     /gene="cas2e"
                     /locus_tag="ACHOSC_RS17690"
                     /old_locus_tag="pgaptmp_003538"
     CDS             8151..>8425
                     /gene="cas2e"
                     /locus_tag="ACHOSC_RS17690"
                     /old_locus_tag="pgaptmp_003538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004521 - RNA endonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I-E CRISPR-associated endoribonuclease
                     Cas2e"
                     /protein_id="HGU4549416.1"
                     /translation="MSMVVVVTENVPPRLRGRLAIWLLEVRAGVYVGDTSKRIREMIW
                     QQITQLAGCGNVVMAWATNTESGFEFQTWGENRRIPVDLDGLRLVSFL"
BASE COUNT         2071 a         2004 c         2341 g         2009 t
ORIGIN      
        1 ggcattcgct ggatgtagcc gcgatgggct atttaatggt taaaagaaat tgcttcgggc
       61 tggctgatta ttttcgtcaa ttagggattt ctgacaagga acaggcggct caatttttcg
      121 cctggttgct gtgctggcac gatattggaa aatttgcccg ctcttttcaa caactttacc
      181 tgccccctga actcaagatt caggaaggtg ccagaaaaaa ttacgaaaaa atctctcatt
      241 caacgctggg ctactggttg tggaatcatt atttaagtga atgtcaggag ttacttcctt
      301 catcttcgct ctctccgcgc aaacttaggc gagtgataga aatgtggatg cccgtgacga
      361 cagggcatca tggtcagccc cctgatcgta tggatgaatt ggataatttt ctgcctgaag
      421 acaaagctgc agcacgagat tttctccttg aaatcaaacc actgtttccg ctcatagaga
      481 tccccgcatt ctgggatgat gatgagggca ttgaacttat aaaacacctt tcctggtata
      541 tctctgcaac cgtcgtgctc gcagactgga cgggttcgtc aacgcgattt tttccacgcg
      601 tcgcacatcc aatggatatt aaaggttact ggcagaaaac tttaattcag gctcaaaacg
      661 ccttaaccgt ctttcctcta aaagcaaaag tcgcgccttt caacggaatt aatacgctgt
      721 ttccttttat tgagaatccg acaccgttac agcaaaaggt gctggatctg gatatcagcc
      781 agcaaggacc gcagttattt attctggaag atgtgactgg cgcaggtaaa acagaagcgg
      841 cgcttatcct ggcgcacagg ttgatagctg cggggaaagc tcaggggctg ttttttggtc
      901 tgccaacaat ggcaacggcc aatgccatgt acgatcggct ggtcaaaacc tggcttgctt
      961 tctattcgcc agaatcccgc cccagcctgg tgctggcaca tagtgcccgc acattaatgg
     1021 accgctttaa tgaatcactc tggtctggtg atttagtcgg gtcagaagaa ccggatgaac
     1081 aaacgttcag tcagggatgt gcggcctggt ttgccgacag taacaagaag gcgttgctgg
     1141 ctgaaattgg cgtcggcacg ctggatcagg cgatgatggc ggtgatgccg ttcaaacata
     1201 ataatctgcg gcttctgggg ctgagtaaca aaatcctgct ggctgatgag atccacgcct
     1261 gtgacgctta catgtcgtgc attcttgaag ggttgatcga gcggcaggcg cgtggcggaa
     1321 acagcgttat tttgctttct gccacgttat ctcaacagca gcgcgacaaa ctcgtcgctg
     1381 cctttgcgcg gggtacagag ggccagcaag aagctccgtt cctggaaaag gatgattacc
     1441 cctggctgac gcatgtcacg aaatccgatg tgaactcaca ccgggtagcg acgcgcaaag
     1501 atgttgagcg tagcgtcagc gtgggttggc ttcatagtga acaagagtgt attgcgcgta
     1561 tcgaatcggc ggtaagtcag gggaaatgca tcgcctggat ccggaattct gtcgatgacg
     1621 ctattaaggt tcatcgtcag ctgcttgccc gcggcgtcat tcctgcttcc agcctttcac
     1681 tttttcatag ccgctttgct tttagcgatc gccagcgaat tgaaacggaa acgctggcac
     1741 gctttggtaa atattgtagt ttacagagag cgtcgcaagt cattgtttgt acacaagtaa
     1801 ttgagcaaag cgtggatatt gacctggacg aaatgatctc cgacctggcc cctgttgatt
     1861 tgctgattca gcgagcagga cgattacagc ggcatatccg cgatattaat ggtcagttaa
     1921 agcgtgacgg aaaggacgag cgttcccccc ctgaattgct gattctggct cccgtctggg
     1981 acgactctcc tggcgacgaa tggtttggca gtgccatgcg taacagtgca tttgtctatc
     2041 ccgatcatgg gcgaatctgg ctgacgcagc gtgtgctgcg tgagcaaggt gctattcaga
     2101 tgccacacgc agcccggctt cttattgaat ccgtctacgg tgaggacgtg gtaatgccgg
     2161 aaggatttgc ccgcagcgag caggagcaag tgggcaaata ttactgcgat cgcgcaatgg
     2221 ctaaaaagtt tgtgctgaac ttcaggcccg gctatgccgc caatatcaac gattacctcc
     2281 cggaaaaact gtcgacacgt ctggctgagg agtctgtttc cctgtggctg gctacctgta
     2341 ttgacggtgt ggtgaagcct tatgccaccg gtgctcacgc atgggaaatg agcgttgtca
     2401 gagtgcgccg aagctggtgg aaaaaacatc gggatgagtt ttctttactg gagggagagg
     2461 cgttcaggct gtggtgcatt gaacagcgac aagatccgga gatggcaaac gtgattttag
     2521 tcaacgatga cgaaagttgc gggtattcgg ctacggaagg attgattggc aaggttggtt
     2581 gagatgtctg cgacaggact ctctgaatag ttgatacacc gaaaaggatg gaaagatgga
     2641 caaccgacct gggatggtct tttttacgag aggatgcgta tgaactcgtt ttcacttctg
     2701 acaaccccgt ggttgcctgt tcgttttaaa gacggaacga caggcaagct ggcaccagtc
     2761 gatctggctg atgaaaatgt tgtcgatatc gctgcgccgc gggcagatct ccagggggcg
     2821 gcctggcagt ttttgctggg gctactacaa accagcttcg cgcccaaaaa tcacggtcgt
     2881 tgggatgata tctggcaaga cggactggag gctgaaaagc tacgggaagc attgctgtcc
     2941 ttagaacacg ctttccagtt tggggcagat tccccttcat ttatgcagga tttcgaggcg
     3001 ctcaagggag ataaagttca ggtcgcttcg ctactgcctg agattcccgg cgctcaaaca
     3061 acgaagttta ataaagacca ctttattaag cgtggcgtga ctgaacacgt atgccctcat
     3121 tgttccgcgt tagctctgtt ctccctacag ttaaatgcgc catcaggcgg caaaggctat
     3181 cgcaccggtt tgcgcggcgg tgggccgatg accactctga ttgaattaca ggaatatcaa
     3241 ggcaatcaac aaacgccctt gtggcgcaaa ctgtggccca atgtgatgcc gcaggatgaa
     3301 gccgacttac cgctacccaa aaaatttgac gatctggttt tcccctggct tggcccgacg
     3361 cgaaccagcg aactggccgg tgcggtggta acccatgatc aggtcaataa actccaggct
     3421 tactggggaa tgccgcgccg tattcgtatt gattttaata ccacgacagt cggcaactgc
     3481 gatatttgcg gtgaacagag cgacgcgctc ctgagcctga tgacgaccaa aaattacggt
     3541 gcgaattatg ccatgtggca gcatccctta acgccttacc gtataccact taaagagggt
     3601 ggcgagtttt actcggtcaa accacaaccg ggcggtttaa tctggcgcga ctggttaggc
     3661 cttatcgaaa cgggtaaatc agaaaacaat acggaacttc ccgcgctggt ggtgaaactc
     3721 tttaatgcca gcagtctgaa acaggcaaaa gtaggcctgt ggggatttgg ctatgatttc
     3781 gacaacatga aagcgcgctg ttggtacgaa caccatttcc cgctgctgct caagaaaaaa
     3841 gaaggccaga taccgaaact gcgtctggct gcgcaaacgg cttcacggat tctaagtctg
     3901 ttacgaagtg cattgaaaga agcgtggttc tccgatccaa aaggtgcacg gggtgatttc
     3961 agttttgtgg atatcgactt ctggaacaaa actcagcatc gcttcctgag gttagtgcgc
     4021 caaattgaag aaggtcagga tgcggacgaa ttactcggca aatggcaaaa ggaaatttgg
     4081 ttattcgcgc gtcaggattt tgacgagcgt gtattcacca atccatatga gcctgttgat
     4141 ttgaaacgcg ttatgaccgc gcgcaagaaa tatttcacaa catcggcgga gaagcaaagt
     4201 gctaaagccg ccagggagaa aaagcaggag gctgctgaat gagtattgta aaagaggagc
     4261 acaaagccac gctgcgtaaa tggcgcgaag agttgcaggc taaacgcggc aatcgtgcaa
     4321 gtctgcggcg tagcacaacg gttaatgatg tttgcttgtc agaagggttt cgttctctgc
     4381 tgatgcagac ccacactttg tggaaaattg aagcacaaga gtggcgtttt accgcactgg
     4441 cgctggtcgc cgcagttagc gccaatgtaa aagctattga tgaacggcag ccttttgccg
     4501 cgcagctggc ggcggtaatg tcaaaggggc gttttactcg tctgtcggca gtaaaaacac
     4561 cggatgaatt actgcgccag ttacgccgcg cagtcagatt attaaacggt tccgtcaatc
     4621 ttgactctct ggcagagggg gtcttccgtt ggtgccagga gagcgacgac ctgctcaatc
     4681 atcaccgccg ccagcagcgg ccaaccgaat ttatccgtat ccgctgagcg ctggaatatt
     4741 accaggctgg cgacgccgat aacgaacaaa atcaataaag gactatctca tgacaacatt
     4801 tattcagctt catttgttaa ccgcttaccc tgctgccaac cttaaccgtg atgataccgg
     4861 tgcgccaaaa actgtggtgc tgggtggagc aacgcgtctg cgcgtttcct cgcaaagtct
     4921 gaaacgtgcg tggcgcactt ctgcactttt tgaacaggca ctggcgggcc atattggtat
     4981 tcgcagtggg cgtattgcgc gtgaggcggc aactatcctg attgagaaag ggattgaaga
     5041 taaaaaagcc atcgaatggt cggcaaaaat tgctgattat cttgggaaag ctaaaaatga
     5101 caaaaaacca aaagatccgc tcactaacgc cgaaactgaa cagttagtcc atatcagccc
     5161 ggcagaattt gacgccgtaa aagcgctggc ccatcagctt gcagaagaaa agcgcgcgcc
     5221 aaaagaggaa gatctcgcgt tgttacgcaa agaccgtatg gcggtagata ttgccatgtt
     5281 tggtcgtatg ctggcgaata aacccgagtt taatgttgaa gccgcctgcc aggtagcgca
     5341 cgcatttggt gtcagtgaaa cgattgtcga agatgatttt ttcaccgccg ttgatgattt
     5401 gcgccaggct tctgaagatg ccggtgccgg gcatcttggt gaaaccggat tcggttctgc
     5461 actgttctac acctatatct gcattgataa agatctgctg gtcgaaaacc ttggcggtga
     5521 cgaagcgtta gctaatcaga ccatacgtgc ctttacggaa gccgcactta aagtctcccc
     5581 aaccggcaaa cagaacagct ttgccagccg tgcctacgcc tcctgggcgc tggcagaaaa
     5641 aggcaccgat caaccacgct ctctggcggc ggctttctat gaacccatta atggcacacg
     5701 tcagttagat gtggcggtgc agcgtattac aacgcttcgc gaaaacatga atacggtcta
     5761 cgaacagaag accgaatgcg caagttttga cgtgatgaac aaacagggaa gcatgaagga
     5821 cgtgctggac tttatctgcg cgtaaggggc ggcttatgag ccaatatctg atttttcagc
     5881 ttcatgggcc gatggcgtcc tgggggggcg atgctccagg tgaagttcgt catacccatg
     5941 aacttccttc gcgctcagca ttgctggggc tactggctgc cggggtaggg attcggcgtg
     6001 atgataccga acgcttaaac gcgtttaatc gccactattc actggtggtt tgcgccagcc
     6061 gtaacccgcg ctgggcacgg gattatcaca cggtccagat gccaaaagag gtgcgtaaag
     6121 cacgttattt cagccgtcgc gaagagttga gcgaccctga gcttttgagc gcgattatct
     6181 cccggcgcga ctactacacc gatgcctggt ggatggtggc ggtggcaaca acagccgatg
     6241 cgccttacag cctcgaacag ttgcaggacg gtttacgcca tccagttttt ccgctttatc
     6301 tggggcgaaa aagtcatccg ctggcattac ctcttgcgcc gttactgctc gaaggcaacg
     6361 cgtgtgatgc cttatgtaat gcataccaac agtatcagga tcatttccat aaactgaaag
     6421 tctcactccc gaaacttcag gatgaatgct ggtgggaagg gaaacacgac ggcctggtcg
     6481 cgagcaaaat attacgccgc cgggatgttc ctttaaatcg tcagcagtgg ctgtttgggg
     6541 aacgcaccgt caatcagggg ccgtggctca gcaaggagga accatgtacc tctcaagaat
     6601 aaccttacat accggccagc tttcgcctgc gcagttgctg catctggtgg atcgcggtga
     6661 atatgtgatg catcagtggc tgtgggatct cttccctggc ggcaaagaaa ggcaatttct
     6721 ttatcgtcgg gaagaactcc agggcgcgtt tcgctttttt gtactgtcgc aggaacgccc
     6781 ggcggaaagt gatacgttca ccatagaatg ccggtcattt gcgcccgagc tgtgtaccgg
     6841 acaacaactt tgtttcaacc tgcgggcgaa tccaacaatc tgtaaagcgg gcaagcgcca
     6901 cgatctgctg atggaggcga aacggcaggt gagggggccg gcggaaggaa gtgatgtctg
     6961 gttgcatcaa caacaggcgg cgctggactg gctggcagcg cagggggaga gaagcggttt
     7021 tacactgctg gatacctcgg ttgatgccta ccgacaacag caactgcggc gggaaaactc
     7081 ccggcaactg atccagttca gtagcgtgga ttatacgggg atgcttacgg tcaccgatcc
     7141 tgggttattt gtgcaacgcc tgagccaggg gtatggtaaa agccgggcgt tcggctgtgg
     7201 tctgatgctg atcaaacccg gagcagaggc gtgacgtttg taccactgag cccgatccca
     7261 ttaaaagatc gcacctccat gatcttcctc cagtacggtc agatcgacgt actggacggc
     7321 gctttcgtgc tgatcgacaa aacagggatc cgaacgcata ttccggtggg atcggtcgcc
     7381 tgcattatgc tcgaaccagg aacgagagtt tcccacgcgg cggtgcatct ggccgccacg
     7441 gtgggaacac tgctggtctg ggtcggtgaa gcgggcgttc gcgtttactc ctccggacaa
     7501 cccggtgggg cgcgggcaga taaattactc taccaggcaa agctggcttt aacggaagat
     7561 ctacggttga aggtggtgcg caaaatgtat gaattacgtt ttcgtgaacc acagccagct
     7621 cgccgttcag tggagcagtt acgtggaatt gagggatctc gcgttcgcca gacgtatgca
     7681 ttactggcga aacaatatgg tgtgaaatgg aatggtcgca aatacgatcc taaagactgg
     7741 gaaaaaggcg atgttgtgaa tcgctgcatc agtgctgcta catcatgtct gtacggtata
     7801 tctgaagcgg cagtattagc cgcgggatat gcgcccgcta ttgggtttat ccatagtggc
     7861 aaaccgcttt catttgttta tgacatagcc gatatcatta aatttgattc ggttgtgcca
     7921 aaggcatttg aaatagcagc gaggcaaccc gcagaacctg ataaagaagt cagattagcc
     7981 tgtcgcgata ttttccgtag cactaagtta acgggcaaat taataccgtt aattgaggaa
     8041 gtccttgccg caggtgaaat tgaaccgcca caacctgcgc cggatatgtt accgccagcc
     8101 atccccgaac ctgaaacgtt gggcgatagc ggtcatcgag gacgcggctg atgagcatgg
     8161 tcgtggttgt tacagaaaat gtcccgccgc gcttacgtgg acggctcgca atctggctac
     8221 tggaagtgcg tgccggtgtg tatgttggtg atacatcaaa acgtattcgg gagatgatct
     8281 ggcagcaaat tacccaactg gctggttgcg gaaatgtggt gatggcctgg gcgaccaata
     8341 ccgagtcggg ttttgaattt cagacctggg gagaaaacag acgtattccg gtggatttgg
     8401 atgggttacg tttggtttct tttct
//
LOCUS       DAZKHF020000190         8419 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2201S31241
            SAMN43267800-rid25106413.denovo.191, whole genome shotgun sequence.
ACCESSION   DAZKHF020000190 DAZKHF020000000
VERSION     DAZKHF020000190.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267800
            Sequence Read Archive: SRR30307263
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 8419)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8419)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 8419)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350581.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350581.2
            Genome Coverage       :: 151x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:21:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,652
            CDSs (total)                      :: 5,557
            Genes (coding)                    :: 5,320
            CDSs (with protein)               :: 5,320
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 237
            CDSs (without protein)            :: 237
            Pseudo Genes (ambiguous residues) :: 0 of 237
            Pseudo Genes (frameshifted)       :: 102 of 237
            Pseudo Genes (incomplete)         :: 130 of 237
            Pseudo Genes (internal stop)      :: 52 of 237
            Pseudo Genes (multiple problems)  :: 41 of 237
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8419
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267800-rid25106413.denovo.191"
                     /serotype="O 26:H11"
                     /isolate="2201S31241"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-01"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            complement(<1..578)
                     /gene="yeeO"
                     /locus_tag="ACHOSC_RS17695"
                     /old_locus_tag="pgaptmp_003539"
     CDS             complement(<1..578)
                     /gene="yeeO"
                     /locus_tag="ACHOSC_RS17695"
                     /old_locus_tag="pgaptmp_003539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001323172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxic metabolite efflux MATE transporter YeeO"
                     /protein_id="HGU4549417.1"
                     /translation="MWFHFLQLRSALNISSALRQVVHGTRWHAKRKSYKVLFWREITP
                     LAVPIFMENACVLLMGVLSTFLVSWLGKDAMAGVGLADSFNMVIMAFFAAIDLGTTVV
                     VAFSLGKRDRRRARVATRQSLVIMTLFAVLLATLIHHFGEQIIDFVAGDATTEVKALA
                     LTYLELTVLSYPAVAITLIGSGALRGAGNTKIP"
     gene            739..814
                     /locus_tag="ACHOSC_RS17700"
                     /old_locus_tag="pgaptmp_003540"
     tRNA            739..814
                     /locus_tag="ACHOSC_RS17700"
                     /old_locus_tag="pgaptmp_003540"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:772..774,aa:Asn,seq:gtt)
     gene            complement(852..1802)
                     /gene="cbl"
                     /locus_tag="ACHOSC_RS17705"
                     /old_locus_tag="pgaptmp_003541"
                     /pseudo
     CDS             complement(852..1802)
                     /gene="cbl"
                     /locus_tag="ACHOSC_RS17705"
                     /old_locus_tag="pgaptmp_003541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416492.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator Cbl"
     gene            complement(1904..2821)
                     /gene="nac"
                     /locus_tag="ACHOSC_RS17710"
                     /old_locus_tag="pgaptmp_003542"
     CDS             complement(1904..2821)
                     /gene="nac"
                     /locus_tag="ACHOSC_RS17710"
                     /old_locus_tag="pgaptmp_003542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrogen assimilation transcriptional regulator
                     NAC"
                     /protein_id="HGU4549418.1"
                     /translation="MNFRRLKYFVKIVDIGSLTQAAEVLHIAQPALSQQVATLEGELN
                     QQLLIRTKRGVTPTDAGKILYTHARAILRQCEQAQLAVHNVGQALSGQVSIGFAPGTA
                     ASSITMPLLQAVRAEFPEIVIYLHENSGAVLNEKLINHQLDMAVIYEHSPVAGVSSQA
                     LLKEDLFLVGTQDCPGQSVDVNAIAQMNLFLPSDYSAVRLRVDEAFSLRRLTAKVIGE
                     IESIATLTAAIASGMGVAVLPESAARSLCGAVNGWMSRITTPSMSLSLSLNLPARANL
                     SPQAQAVKELLMSVISSPVMEKRQWQLVS"
     gene            3147..3222
                     /locus_tag="ACHOSC_RS17715"
                     /old_locus_tag="pgaptmp_003543"
     tRNA            3147..3222
                     /locus_tag="ACHOSC_RS17715"
                     /old_locus_tag="pgaptmp_003543"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:3180..3182,aa:Asn,seq:gtt)
     gene            complement(3266..4213)
                     /gene="ldtA"
                     /locus_tag="ACHOSC_RS17720"
                     /old_locus_tag="pgaptmp_003544"
     CDS             complement(3266..4213)
                     /gene="ldtA"
                     /locus_tag="ACHOSC_RS17720"
                     /old_locus_tag="pgaptmp_003544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001255285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase"
                     /protein_id="HGU4549419.1"
                     /translation="MMRRVNILCSFALLFASHTSLAVTYPLPPEGSRLVGQSLTVTVP
                     DHNTQPLETFAAQYGQGLSNMLEANPGADVFLPKSGSQLTIPQQLILPATVRKGIVVN
                     VAEMRLYYYPPDSNTVEVFPIGIGQAGRETPRNWVTTVERKQEAPTWTPTPNTRREYA
                     KRGESLPAFVPAGPDNPMGLYAIYIGRLYAIHGTNANFGIGLRVSQGCIRLRNDDIKY
                     LFDNVPVGTRVQIIDQPVKYTTEPDGSNWLEVHEPLSRNRAEYESDRKVPLPVTPSLR
                     AFINGQEVDVNRANAALQRRSGMPVQISSGSRQMFYERW"
     gene            complement(4275..5354)
                     /gene="cobT"
                     /locus_tag="ACHOSC_RS17725"
                     /old_locus_tag="pgaptmp_003545"
     CDS             complement(4275..5354)
                     /gene="cobT"
                     /locus_tag="ACHOSC_RS17725"
                     /old_locus_tag="pgaptmp_003545"
                     /EC_number="2.4.2.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008939 -
                     nicotinate-nucleotide-dimethylbenzimidazole
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate-nucleotide--dimethylbenzimidazole
                     phosphoribosyltransferase"
                     /protein_id="HGU4549420.1"
                     /translation="MQTLADLLNTIPAIDPAAMSRAQRHIDGLLKPVGSLGRLEALAI
                     QLAGMPGLNGIPHVGKKAVLVMCADHGVWEEGVAISPKEVTAIQAENMTRGTTGVCVL
                     AAQAGANVHVVDVGIDSAEPIPGLINMRVARGSGNIASAPAMSRRQAEKLLLDVICYT
                     RELAKNGVTLFGVGELGMANTTPAAAIVSTITGRDPEEVVGIGANLPTDKLANKIDVV
                     RRAITLNQPNPQDGVDVLAKVGGFDLVGMAGVMLGAASCGLPVLLDGFLSYAAALAAC
                     QMSPAIKPYLIPSHLSAEKGARIALSHLGLEPYLNMEMRLGEGSGAALAMPIIEAACA
                     IYNNMGELAASNIVLPGNTTSDLNS"
     gene            complement(5366..6109)
                     /gene="cobS"
                     /locus_tag="ACHOSC_RS17730"
                     /old_locus_tag="pgaptmp_003546"
     CDS             complement(5366..6109)
                     /gene="cobS"
                     /locus_tag="ACHOSC_RS17730"
                     /old_locus_tag="pgaptmp_003546"
                     /EC_number="2.7.8.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008818 - cobalamin 5'-phosphate synthase
                     activity [Evidence IEA];
                     GO_function: GO:0051073 - adenosylcobinamide-GDP
                     ribazoletransferase activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylcobinamide-GDP ribazoletransferase"
                     /protein_id="HGU4549421.1"
                     /translation="MSKLFWAMLSFITRLPVPRRRSQGLDFEHYSRGIITFPLIGLLL
                     GAISGLVFMVLQAWCGVPLAALFSVLVLALMTGGFHLDGLADTCDGVFSARSRDRMLE
                     IMRDSRLGTHGGLALIFVVLAKILVLSELALRGEPILASLAAACAVSRGTAALLMYRH
                     RYAREEGLGNVFIGKIDGRQTCVTLGLAAIFAAVLLPGMHGVAAMVVTMVAIFILGQL
                     LKRTLGGQTGDTLGAAIELGELVFLLALL"
     gene            complement(6106..6651)
                     /gene="cobU"
                     /locus_tag="ACHOSC_RS17735"
                     /old_locus_tag="pgaptmp_003547"
     CDS             complement(6106..6651)
                     /gene="cobU"
                     /locus_tag="ACHOSC_RS17735"
                     /old_locus_tag="pgaptmp_003547"
                     /EC_number="2.7.1.156"
                     /EC_number="2.7.7.62"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0043752 - adenosylcobinamide kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional adenosylcobinamide
                     kinase/adenosylcobinamide-phosphate guanylyltransferase"
                     /protein_id="HGU4549422.1"
                     /translation="MMILVTGGARSGKSRHAEALIGDSSQVLYIATSQILDDEMAARI
                     EHHRQGRPEHWRTVERWQHLDELIHADINPNEVVLLECVTTMVTNLLFDYGGDKDPDE
                     WDYQAMEQAINAEIQSLIAACQRCPAKVVLVTNEVGMGIVPESRLARHFRDIAGRVNQ
                     QLAAAANEVWLVVSGIGVKIK"
     gene            complement(6803..6976)
                     /locus_tag="ACHOSC_RS17740"
                     /old_locus_tag="pgaptmp_003548"
                     /pseudo
     CDS             complement(6803..6976)
                     /locus_tag="ACHOSC_RS17740"
                     /old_locus_tag="pgaptmp_003548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001524956.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="cobalamin biosynthesis protein"
     gene            complement(6955..7143)
                     /locus_tag="ACHOSC_RS17745"
                     /old_locus_tag="pgaptmp_003549"
                     /pseudo
     CDS             complement(6955..7143)
                     /locus_tag="ACHOSC_RS17745"
                     /old_locus_tag="pgaptmp_003549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012904641.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS4 family transposase"
     gene            complement(8140..8419)
                     /locus_tag="ACHOSC_RS17750"
                     /old_locus_tag="pgaptmp_003550"
                     /pseudo
     CDS             complement(8140..8419)
                     /locus_tag="ACHOSC_RS17750"
                     /old_locus_tag="pgaptmp_003550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477168.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
BASE COUNT         2271 a         2245 c         1809 g         2094 t
ORIGIN      
        1 ggtattttcg tattccctgc accacgaagt gccccgctac caatcaaagt gatggcaact
       61 gctggataac tgagtaccgt cagctccaga taagtcaacg ccagtgcttt aacttctgtc
      121 gtggcatcac ccgcgacgaa atcaataatt tgttcgccaa aatgatgaat aagcgttgcc
      181 aacagtacgg caaacaacgt catgatcacc aatgactgcc gcgtcgccac cctcgctcgt
      241 cgtcgatccc gcttaccgag actaaatgcc acaacgacag tagtaccaag atcgatagca
      301 gcaaaaaaag ccataatgac catattgaag ctgtccgcca atcccacgcc ggccatcgca
      361 tcttttccca gccagctgac cagaaaagtg ctcaggacac ccatcaacag gacacaggca
      421 ttctccatga agataggaac agcaagcggg gttatctcgc gccagaacaa cactttgtag
      481 ctcttgcgtt tagcgtgcca gcgagtgccg tgaacaacct ggcgtaaagc agaggagata
      541 ttcaaagccg accttaattg cagaaagtga aaccacattt caaataatga gggagaatca
      601 gcaaagctgc aaagattttc gccaacaaat tgtctgcaaa tgcaacaaac tgttgataga
      661 aacggcaaac agttgggaaa tttaaaaatc gggtttgaca aaagattttt cgccgttaag
      721 atgtgcctca acagcgattc ctctgtagtt cagtcggtag aacggcggac tgttaatccg
      781 tatgtcactg gttcgagtcc agtcagagga gccaaatttc tgaaaagccc gcttttatag
      841 cgggcttttt gctatatctg ataatcaatt tcctcttcac tgttctccat cacctggcgc
      901 ttgatatcct caactgacag tcctgcatta caaagttcca gaaagcgcca gacatagtta
      961 cgttgaagtt gccctcgctt cagtcccaac catacagtat tggcatcgaa aagatgccgc
     1021 gtatccagac ggattaaatt ctcttcctct tgttcgccgc tggattgttc ggcaactaat
     1081 ccaatcccca gcccaagagc aacataggtt ttaatgacat cagaatcctg cgcacttaat
     1141 acaatatctg ccagcaaacc tttgcgggca aatgcgtcat caatacgtga gcaccccgta
     1201 atcccctatc ggtaagtgat taacggccac ttcgctattg attccagcgt caacggtgta
     1261 atttgcgtca agggatgatc gagtggaaca agcaaactat ggtgccaacg aaaccacggg
     1321 aaggcgacga gctgcgggtc attactcaaa cgctcgctgg cgataccaat atcagcttcg
     1381 ccattttgca acaacgtcgc aatttcctgt ggcgtaccct ggattagctc cagccgaacc
     1441 tccgggaaaa gttcgcgaaa agctttaatg acctcaggca agctataacg tgcctgagta
     1501 tgcgtcgttg caatagtgag aacgccagac gtatcgttgg taaacaggtc tgcaagccga
     1561 cgaacattac tggcttcatt cagaatacgt tctgcaatga ccagtaatgc tttgcccggt
     1621 tcagtcatac ccagcagtcg cttacctcgt cgaacaaata tttcgatgcc aagttcatcc
     1681 cccagttccc gaatatgacg gctgacgccg gactgtgagg taaaaagcat attcgcaacc
     1741 tctgtcaggt tgtaatcctg acgtgcagcc tcgcggatta tctttagttg ttggaaattc
     1801 acggtaaact ccgggcagct cagatttccc gttattgtta aagtctaatg cccggcataa
     1861 caaataataa aaacccgcat cttattccat cccgatataa cgcttagctc accaattgcc
     1921 actgcctttt ttccatcact ggagaactaa tcactgacat taacaactct ttcactgcct
     1981 gtgcctgtgg cgataagttc gctctggcgg gtaaatttaa tgacaaagag agactcatgg
     2041 aaggcgtggt aatgcgtgac atccacccat ttactgcgcc acataacgaa cgcgcggccg
     2101 attcgggtaa tactgcaacg cccatgccgc tggcaatcgc tgcggtaagc gtggcaatag
     2161 actcaatttc accaatgact tttgccgtga gccgccgtag ggaaaaagcc tcatcaacac
     2221 gaagtctaac agcactgtaa tcactgggga gaaagaggtt catttgcgca atagcattaa
     2281 catcaacgct ttgccccggg caatcttgag ttcctaccag gaaaagatct tctttcagca
     2341 aagcctgact ggatacccca gccacagggg aatgctcata aatcaccgcc atatcgagtt
     2401 ggtgatttat cagtttttcg ttaagcactg caccgctatt ttcatgaaga tagataacga
     2461 tctccgggaa ttcagcgcga accgcttgta ataagggcat ggtgatagat gacgcagcgg
     2521 ttcctggcgc aaagccaatc gagacttgcc ccgataatgc ctgaccaacg ttatgcaccg
     2581 ccagttgggc ctgctcacac tgacgtaaaa tggcccgcgc atgggtatag agaatttttc
     2641 cggcgtctgt tggcgtaacg ccccgttttg tgcgaatcaa aagttgttga tttaactcac
     2701 cttccagtgt ggcaacctgc tggctgagcg ctggttgtgc gatatgcaat acttcagcag
     2761 cctgagtcag gctaccaata tctacaattt ttacgaagta tttcaggcgt ctgaagttca
     2821 tgttgcctcc ggtttttgag aatcggccca agtgccgcca ttacttacaa ccagactgca
     2881 agatgcttgc cagttttatt ttggcgttga tgtacaagct aaccaactgt caaataagag
     2941 attatgatag attcgtcatt ggctcttttc accagcagtc gcgttcccct gccctataaa
     3001 aggagggtat gcaccacgat ggttcattac caataagatt gaaatctcat cactttgttg
     3061 aaattggcag caaacaaaca aaaaaatgca tttcaccctt tgacatcacc atgcactgcc
     3121 attaatatgc gccccgttca cacgattcct ctgtagttca gtcggtagaa cggcggactg
     3181 ttaatccgta tgtcactggt tcgagtccag tcagaggagc caaattcaaa aaagcctgct
     3241 ttctagcagg atttttgctt tctaattacc aacgctcata aaacatctgt cttgaaccag
     3301 aactaatttg cacaggcatt cccgatcgac gttgcaacgc agcatttgcg cgattcacat
     3361 caacttcttg cccgttgata aacgcccgca aagatggggt taccggcaat ggcacttttc
     3421 ggtcagactc atattctgca cgattgcgcg acagcggctc atgaacttcc agccagttcg
     3481 agccatctgg ttcagtggtg tattttactg gctggtcgat aatttgcaca cgcgttccaa
     3541 caggaacatt atcaaacaga tatttgatat cgtcattgcg cagacgaata cagccctgac
     3601 ttacccgaag tccaatacca aaattggcat tggtaccgtg gatggcatac aacctgccaa
     3661 tataaatcgc gtacagcccc atgggattat cgggccccgc aggaacaaat gcgggcaaac
     3721 tctcccctcg tttcgcatat tcgcgccgag tgttcggcgt tggcgtccag gttggagctt
     3781 cttgtttacg ttcaacggta gtcacccagt tacgcggggt ttctcgccca gcctggccga
     3841 taccaatagg aaagacttcc acagtattac tgtctggcgg gtagtaataa agacgcatct
     3901 cagcgacgtt aaccacaatc cctttacgaa cagtggcggg caaaatcagt tgctgcggaa
     3961 tggtgagttg cgagccagac ttcggcaaga aaacatcagc gcccggattc gcttccagca
     4021 tgttacttaa cccttgcccg tattgtgcgg caaaagtctc cagcggctgg gtattgtgat
     4081 caggaacagt tacagtaagc gactgcccca ccaaacggct accctctgga ggtaatggat
     4141 aagttaccgc caggctagta tggctggcaa aaagcagagc aaatgagcaa agaatattta
     4201 cacgacgcat catgtccctt tcctatgtcg cgaaagctat ccgttaagta tagcttttat
     4261 cagacttttc gtttttaact gttcaaatca gaagtcgtat tccccggtag aacaatatta
     4321 ctggcagcaa gttcgcccat gttgttatat atcgcacagg cagcttcgat gatgggcatc
     4381 gccagagctg cgccactccc ctcacctaaa cgcatctcca tattgagata aggctccagc
     4441 cccaaatgcg acagcgctat acgcgcccct ttttctgccg acaggtgaga aggaatgaga
     4501 tacggtttga ttgcaggaga catctggcag gctgcgagcg cagcagcata agaaagaaat
     4561 ccatccagca acacgggtaa accgcaggaa gcagcgccta acatcacgcc agccattccg
     4621 accaaatcaa atccccccac ttttgccaga acatcaacgc catcctgcgg atttggttga
     4681 ttcaacgtaa tcgcccggcg cacaacatca attttattag ccagtttatc tgtcggcagg
     4741 tttgcgccaa tcccaaccac ttcttcagga tcccggccag tgattgtgct gactattgcc
     4801 gctgccggtg tcgtgtttgc catccccagt tcacctacac caaacagcgt gacaccgttt
     4861 tttgccagct cccgcgtata acatatgacg tccaaaagca acttttcagc ctgacggcga
     4921 ctcattgccg gagctgaagc aatattgccg ctacctcgtg cgacacgcat gttgataagc
     4981 ccggggatag gctcagcact atcaatacca acatcaacta cgtggacgtt agcgcccgct
     5041 tgtgctgcca gcacacacac gccggttgtt ccgcgggtca tattttcagc ctgaatggct
     5101 gtcacttctt ttggggaaat agcgacccct tcctcccaga cgccgtgatc ggcacacata
     5161 accagtaccg cttttttgcc cacatgcggt atgccattca accccggcat tcccgccagt
     5221 tgtatggcaa gcgcctccag ccttcccagg ctaccaacag gtttgagtaa cccgtcaata
     5281 tgccgttgtg cacgcgacat agcggcagga tcgatagcag ggatcgtatt cagtaaatcg
     5341 gcaagtgttt gcatgtcacg tctctttata gcagtgccag caggaagacc agttcaccaa
     5401 gctctatagc agctcccagc gtatcgccgg tttgcccgcc cagcgtacgt ttgagaagct
     5461 gaccgaggat gaaaatcgcc accatcgtca ccaccatagc agccacacca tgcatacccg
     5521 gcaataatac ggcagcaaaa atagcggcta agccgagtgt gacgcaggtt tgtcgcccat
     5581 caattttgcc aatgaacaca ttgccaagcc cttcttcccg ggcataacga tgacgataca
     5641 tcaataatgc ggcagtcccg cggctgaccg cacatgctgc cgccagcgat gcaaggatcg
     5701 gttcgccacg cagagccaac tcgcttaata ccagaatctt tgccagtaca acaaaaatca
     5761 atgccagacc gccatgggtg cctaaacggc tatcacgcat gatttccaac atgcgatcgc
     5821 ggctacgtgc agaaaatacg ccatcacagg tatcggcgag gccgtctagg tggaatcccc
     5881 cggtcatcag cgccagcaca agtacgctaa acagtgccgc cagtggtacg ccacaccatg
     5941 cctgcagcac catgaagacc agcccgctaa tcgcgccaag caataatcca attaaaggaa
     6001 aagtaataat accgcgagaa taatgctcga aatccagtcc ctgggacctg cgacgcggta
     6061 caggcagacg cgtaataaat gagagcatcg cccaaaataa tttactcatt tgatttttac
     6121 tccaataccc gaaaccacca gccatacttc atttgccgca gcggccaact gctgatttac
     6181 ccgcccggca atatcacgaa aatgtcgtgc cagacgactc tccggcacaa tccccattcc
     6241 cacttcgtta gtcactaata caacctttgc ggggcaacgt tggcaggcag caatcaacga
     6301 ctgaatctca gcattaatcg cctgttccat cgcctgataa tcccattcat cagggtcttt
     6361 atcgccgcca taatcaaaca acagattagt caccattgtg gtaacgcatt caagcaacac
     6421 aacctcattc gggttaatgt ctgcatgaat taattcatca agatgttgcc agcgctccac
     6481 tgtgcgccag tgctccgggc ggccttgccg atgatgttct atccgtgcag ccatctcatc
     6541 atcaaggatt tgcgaggtag cgatatacag aacctgtgaa gagtccccaa taagcgcctc
     6601 tgcgtggcga ctcttcccgc tccgtgcgcc ccccgtcacc aaaatcatca taccgactcc
     6661 tgattcaaat gtatgatggt attcattgaa cagaacaatg gatcccccac cgttgctgac
     6721 cgcaaaacat ttacgcgatt aatactgcgc gtaatataat taaaaatcca gcattctcaa
     6781 tccattacga aataagatgg cattacggaa tagccagact ttctcgtgtt aacgtccgac
     6841 aaaatgcgtc attgacagcg gtttctgtgg attgacacat ttcattcaga taatgaatta
     6901 atgctactgc cggaacaacc agcaaacggg cattgtgttc tgaaatccag gctattgatt
     6961 caacgtcagc gacagatctg cgctgtaaaa gaacttgtaa aacaacgtaa tagaattatc
     7021 cggaatggtg gcgactatgc actagaacac cacaatttcg ctctctcggt aaggcactgt
     7081 cacagcattc aacagaatgt gacttgccag atttattagc gccaccagat gtttaaccgg
     7141 gtaaccacga cccagtcggg gatatattcc atcttctcgc tgacatcatc aagaataaaa
     7201 aggtttatca cactcagagc aacgttcttc agcaggcggg ataattcttc aacatttaga
     7261 agatgtgtta ttaaaggcat acaaactgga cgtattgtat tttcttttgg tggttgacct
     7321 aaggtagcag tttatcctga tgcgctgaga tttctgtaat atctacgtca acattctcct
     7381 cgaatagaaa atgcagcccc ggcaagtgat tcacattttt taacaaaacg ttgttagcgt
     7441 gccattttca gaagctctcc aagaaagcga attcttttct caaattctgc attctcagac
     7501 acagctttct actccatggt aatggcccaa atacgaagtt gctcaggatc attagtggtg
     7561 ctgagaagtt aaataaccat gcggagtacc actcgtcatc acgtatttat atatcatgaa
     7621 gctattgttg tgttttgtac atcagtagat atattctgat atactccttt tgctagacat
     7681 aacctttcac ctgcttgcaa agcttctgtg ttctgacatt gccaaattgt tgcaattctg
     7741 tatccagcct tctttcagtc atagcttcgg gccgcgataa gactcactga tctgaccctg
     7801 attcctcttg cagactttat agaccaatta aaatgcagtt tctgcaggtc aacgtctgac
     7861 catcattgtc atcactctgg ccattagagt aaccttctgc attcatcctt ttgtaaaaag
     7921 tttatgtcag tatcagcaat taaccggacc tgatactgat atgagtctta ccgcatatac
     7981 ggtcaatttc agcaattaat tacattatcc acgccaaagt gtttgtcatc gcaatgatgg
     8041 taccttcttt cagacaccat tttttcaact ccgttttcca cggaccgcac tcttatgtca
     8101 agagtgcggt gatcctaccc acgtaatatg gacacaggcc taagcgaggt tctggttttc
     8161 aaattgttcc ggactgaggc cgccacacca actgtgccgc cgccaccgat tgtaatcaca
     8221 ttcgatataa ttaaacaccg ttgcccgcat tatttcccgg ctgataaagt gttctccatg
     8281 gatacattcc actttcagcg aatgaaagaa gctttccacg cagattatgc cgcttcagtt
     8341 gcgcctgata atctgctgaa cagtactggc ctccacggtc cgtgtgaacg ataacgttcc
     8401 ggggcctctt acgccgcca
//
LOCUS       DAZKHF020000191         8343 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2201S31241
            SAMN43267800-rid25106413.denovo.192, whole genome shotgun sequence.
ACCESSION   DAZKHF020000191 DAZKHF020000000
VERSION     DAZKHF020000191.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267800
            Sequence Read Archive: SRR30307263
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 8343)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8343)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 8343)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350581.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350581.2
            Genome Coverage       :: 151x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:21:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,652
            CDSs (total)                      :: 5,557
            Genes (coding)                    :: 5,320
            CDSs (with protein)               :: 5,320
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 237
            CDSs (without protein)            :: 237
            Pseudo Genes (ambiguous residues) :: 0 of 237
            Pseudo Genes (frameshifted)       :: 102 of 237
            Pseudo Genes (incomplete)         :: 130 of 237
            Pseudo Genes (internal stop)      :: 52 of 237
            Pseudo Genes (multiple problems)  :: 41 of 237
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8343
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267800-rid25106413.denovo.192"
                     /serotype="O 26:H11"
                     /isolate="2201S31241"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-01"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            complement(77..283)
                     /locus_tag="ACHOSC_RS17755"
                     /old_locus_tag="pgaptmp_003551"
     CDS             complement(77..283)
                     /locus_tag="ACHOSC_RS17755"
                     /old_locus_tag="pgaptmp_003551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbfA family protein"
                     /protein_id="HGU4549423.1"
                     /translation="MELYREYPAWLIFLRRTYAVAAGVLALPFMLFWKDRARFYSYLH
                     RVWSKTSDKPVWMDQAEKATGDFY"
     gene            596..685
                     /gene="kdpF"
                     /locus_tag="ACHOSC_RS17760"
                     /old_locus_tag="pgaptmp_003552"
     CDS             596..685
                     /gene="kdpF"
                     /locus_tag="ACHOSC_RS17760"
                     /old_locus_tag="pgaptmp_003552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="K(+)-transporting ATPase subunit F"
                     /protein_id="HGU4549424.1"
                     /translation="MSAGVITGVLLVFLLLGYLVYALINAEAF"
     gene            685..2358
                     /gene="kdpA"
                     /locus_tag="ACHOSC_RS17765"
                     /old_locus_tag="pgaptmp_003553"
     CDS             685..2358
                     /gene="kdpA"
                     /locus_tag="ACHOSC_RS17765"
                     /old_locus_tag="pgaptmp_003553"
                     /EC_number="7.2.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706533.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031004 - potassium ion-transporting
                     ATPase complex [Evidence IEA];
                     GO_function: GO:0008556 - P-type potassium transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="potassium-transporting ATPase subunit KdpA"
                     /protein_id="HGU4549425.1"
                     /translation="MAAQGFLLIATFLLVLMVLARPLGSGLARLINDIPLPGTAGVER
                     ILFRLPGVSDHEMNWKQYLCAILGLNMLGLAVLFFMLLDQHYLPLNPQQLPGLSWDLA
                     LNAAVSFVTNTNWQSYSGETTLSYFSQMAGLTVQNFLSAASGIAVIFALIRAFTRQSM
                     STLGNAWVDLLRITLWVLVPVALLIALFFIQQGALQNFQPYQAVNTVEGAQQLLPMGP
                     VASQEAIKMLGTNGGGFFNANSSHPFENPTALTNFVQMLAIFLIPTALCFAFGEVTGD
                     RRQGRMLLWAMSVIFVICVGVVMWAEVQGNPHLLALGADSSINMEGKESRFGVLVSSL
                     FAVVTTAASCGAVIAMHDSFTALGGMVPMWLMQIGEVVFGSVGSGLYGMMLFVLLAVF
                     IAGLMIGRTPEYLGKKIDVREMKLTALAILVTPTLVLMGAALAMMTDAGRSAMLNPGP
                     HGFSEVLYAVSSAANNNGSAFAGLSANSPFWNCLLAFCMFVGRFGVIIPVMAIAGSLV
                     SKKSQPASSGTLPTHGPLFVGLLIGTVLLVGALTFIPALALGPVAEYLS"
     gene            2381..4429
                     /gene="kdpB"
                     /locus_tag="ACHOSC_RS17770"
                     /old_locus_tag="pgaptmp_003554"
     CDS             2381..4429
                     /gene="kdpB"
                     /locus_tag="ACHOSC_RS17770"
                     /old_locus_tag="pgaptmp_003554"
                     /EC_number="7.2.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0031004 - potassium ion-transporting
                     ATPase complex [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008556 - P-type potassium transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="potassium-transporting ATPase subunit KdpB"
                     /protein_id="HGU4549426.1"
                     /translation="MSRKQLALFEPTLVVQALKEAVKKLNPQAQWRNPVMFIVWIGSL
                     LTTCISIAMASGAMPGNALFSAAISGWLWVTVLFANFAEALAEGRSKAQANSLKGVKK
                     TAFARKLREPKYGAAADKVPADQLRKGDIVLVEAGDIIPCDGEVIEGGASVDESAITG
                     ESAPVIRESGGDFASVTGGTRILSDWLVIECSVNPGETFLDRMIAMVEGAQRRKTPNE
                     IALTILLIALTIVFLLATATLWPFSARGGNAVSVTVLVALLVCLIPTTIGGLLSAIGV
                     AGMSRMLGANVIATSGRAVEAAGDVDVLLLDKTGTITLGNRQASEFIPAQGVDEKTLA
                     DAAQLASLADETPEGRSIVILAKQRFNLRERDVQSLHATFVPFTAQSRMSGINIDNRM
                     IRKGSVDAIRRHVEANGGHFPTDVDQKVDQVARQGATPLVVVEGSRVLGVIALKDIVK
                     GGIKERFAQLRKMGIKTVMITGDNRLTAAAIAAEAGVDDFLAEATPEAKLALIRQYQA
                     EGRLVAMTGDGTNDAPALAQADVAVAMNSGTQAAKEAGNMVDLDSNPTKLIEVVHIGK
                     QMLMTRGSLTTFSIANDVAKYFAIIPAAFAATYPQLNALNIMRLHSPDSAILSAVIFN
                     ALIIVFLIPLALKGVSYKPLTASAMLRRNLWIYGLGGLLVPFIGIKVIDLLLTVCGLV
                     "
     gene            4438..5010
                     /gene="kdpC"
                     /locus_tag="ACHOSC_RS17775"
                     /old_locus_tag="pgaptmp_003555"
     CDS             4438..5010
                     /gene="kdpC"
                     /locus_tag="ACHOSC_RS17775"
                     /old_locus_tag="pgaptmp_003555"
                     /EC_number="7.2.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031004 - potassium ion-transporting
                     ATPase complex [Evidence IEA];
                     GO_function: GO:0008556 - P-type potassium transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="K(+)-transporting ATPase subunit C"
                     /protein_id="HGU4549427.1"
                     /translation="MRGLRPALSTFIFLLLITGGVYPLLTTVLGQWWFPWQANGSLIR
                     EGDTVRGSALIGQNFTGNGYFQGRPSATAEMPYNPQASGGSNLAVSNPELDKQIAARV
                     AALRAANPDASTNVPVELVTASASGLDNNITPQAAVWQIPRVAKARNLSVEQLTQLIA
                     KYSQQPLVKYIGQPVVNIVELNLALDKLDE"
     gene            5003..7687
                     /gene="kdpD"
                     /locus_tag="ACHOSC_RS17780"
                     /old_locus_tag="pgaptmp_003556"
     CDS             5003..7687
                     /gene="kdpD"
                     /locus_tag="ACHOSC_RS17780"
                     /old_locus_tag="pgaptmp_003556"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     KdpD"
                     /protein_id="HGU4549428.1"
                     /translation="MNNEPLRPDPDRLLEQTAAPHRGKLKVFFGACAGVGKTWAMLAE
                     AQRLRAQGLDIVVGVVETHGRKDTAAMLEGLAVLPPKRQAYRGRHISEFDLDAALARR
                     PALILMDELAHSNAPGSRHPKRWQDIEELLEAGIDVFTTVNVQHLESLNDVVSGVTGI
                     QVRETVPDPFFDAADDVVLVDLPPDDLRQRLKEGKVYIAGQAERAIEHFFRKGNLIAL
                     RELALRRTADRVDEQMRAWRGHPGEEKVWHTRDAILLCIGHNTGSEKLVRAAARLASR
                     LGSVWHAVYVETPALHRLPEKKRRAILSALRLAQELGAETATLTDPAEEKAVVRYARE
                     HNLGKIILGRPASCRWWRRETFADRLARIAPDLDQVLVALDEPPARTINNAPDSRSFK
                     DKWRVQIQGCVVAAALCAVITLIAMQWLMAFDAANLVMLYLLGVVVVALFYGRWPSVV
                     ATVINVVSFDLFFIAPRGTLAVSDVQYLLTFAVMLTVGLVIGNLTAGVRYQARVARYR
                     EQRTRHLYEMSKALAVGRSPQDIAATSEQFIASTFHARSQVLLPDDNGKLQPLTHPQG
                     MTPWDDAIAQWSFDKGLPAGAGTDTLPGVPYQILPLKSGEKTYGLVVVEPGNLRQLMI
                     PEQQRLLETFTLLVANAFERLTLTASEEQARMASEREQIRNALLAALSHDLRTPLTVL
                     FGQAEILTLDLASEGSPHARQASEIRQHVLNTTRLVNNLLDMARIQSGGFNLKKEWLT
                     LEEVVGSALQMLEPGLSSPINLSLPEPLTLIHVDGPLFERVLINLLENAVKYAGAQAE
                     IGIDAHVEGENLQLDVWDNGPGLPPGQEQTIFDKFARGNKESAVPGVGLGLAICRAIV
                     DVHGGTITAFNRPEGGACFRVTLPQQTAPELEDFHEDM"
     gene            7684..>8343
                     /gene="kdpE"
                     /locus_tag="ACHOSC_RS17785"
                     /old_locus_tag="pgaptmp_003557"
     CDS             7684..>8343
                     /gene="kdpE"
                     /locus_tag="ACHOSC_RS17785"
                     /old_locus_tag="pgaptmp_003557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator KdpE"
                     /protein_id="HGU4549429.1"
                     /translation="MTNVLIVEDEQAIRRFLRTALEGDGMRVYEAETLQRGLLEAATR
                     KPDLIILDLGLPDGDGIEFIRDLRQWSAVPVIVLSARSEESDKIAALDAGADDYLSKP
                     FGIGELQARLRVALRRHSATAAPNPLVKFSDVTVDLAARVIHRGEEEVHLTPIEFRLL
                     AVLLNNAGKVLTQRQLLNQVWGPNAVEHSHYLRIYMGHLRQKLEQDPARPRHFITETG
                     IG"
BASE COUNT         1732 a         2173 c         2439 g         1999 t
ORIGIN      
        1 cctctgaaca tcttcctgat acaaaaaacc gccagcgttt ccgctggcgg ttttcaatgt
       61 attcaaaact cagcaatcaa taaaaatcac cagttgcctt ttcggcctga tccatccaca
      121 ccggtttatc gctggttttc gaccagacgc gatgcaggta gctgtaaaag cgggcgcggt
      181 ctttccagaa aagcatgaaa ggcagcgcca gaacgcccgc tgcaaccgca taagtacggc
      241 gtaaaaagat aagccatgca ggatattctc tgtagagttc catacttttc tccccctttt
      301 gtatctaccc ggtgaatggt accggaaaaa tgaatttgtt tatctgatga aaatagtacc
      361 gccttttgtg taattttact actcatccga ccacttattt ttgcttattg atggtttatt
      421 tacattcatc ctgtaattaa gttacacaaa agttaaatta atactaaaca ttagttaaat
      481 catggctttt gccattttta tacttttttt acaccccgcc cgcagatttt tgcgaaatct
      541 ttgcagccag aattctaccc ttccggtatc acttttaggc cactggaggt gcactgtgag
      601 tgcaggcgtg ataaccggcg tattgctggt gtttttatta ctgggttatc tggtttatgc
      661 cctgatcaat gcggaggcgt tctgatggct gcgcaagggt tcttactgat cgccacgttt
      721 ttactggtgt taatggtgct ggcgcgtcct ttaggcagcg ggctggcgcg gctgattaat
      781 gacattcctc ttccaggtac agcgggcgtt gaacgtatac tctttcgttt acctggcgtc
      841 tctgaccatg agatgaactg gaagcaatat ctttgtgcca ttctcggcct gaacatgctg
      901 gggctggcgg tgctgttttt tatgttgctc gatcagcact atctgccgct taatccacag
      961 cagttgccgg ggctgtcgtg ggatctggcg ctgaatgccg ccgtcagctt tgtcacgaat
     1021 accaactggc aatcttacag cggtgaaacc acgttgagct atttcagcca gatggctggc
     1081 ttaacggtgc aaaactttct ttctgccgcc agcgggattg cggtgatttt tgccctcatc
     1141 cgtgcgttta cccgccagag catgagcaca ctcgggaatg cctgggtcga tctgctacgc
     1201 atcacgttat gggtgctcgt ccctgtggcg ttgttgatcg cactgttttt tattcaacaa
     1261 ggtgcgctgc aaaactttca gccttatcag gctgtgaata ccgttgaagg agcgcaacag
     1321 ctgttaccca tggggcctgt agcttctcag gaagcgatca agatgctcgg tactaacggc
     1381 ggtggcttct ttaatgccaa ctcgtcgcat ccgtttgaaa acccaaccgc actgaccaac
     1441 ttcgtgcaga tgctggcgat cttcttgatc ccaacggcgc tgtgctttgc ctttggtgaa
     1501 gtgacgggcg atcgccgcca ggggcgcatg ttgctgtggg cgatgtcagt gatttttgtc
     1561 atctgcgtag gcgtggtgat gtgggcagaa gttcagggta atcctcatct gctggcgctc
     1621 ggcgcagaca gcagcatcaa tatggaaggt aaagagagcc gtttcggcgt gctggtcagt
     1681 agcctgtttg cggtcgtgac tacggcggct tcctgtggcg cggtgattgc gatgcatgat
     1741 tcgtttaccg ctctcggtgg catggtgccg atgtggctga tgcaaattgg tgaagtggtg
     1801 ttcggcagtg tcggttctgg tctttacggc atgatgctgt ttgtcctgct ggcggtgttt
     1861 attgccgggc tgatgattgg tcgtacaccg gaatatctgg gtaaaaaaat cgacgtacgc
     1921 gagatgaaac tgactgcact ggcgattctg gttaccccga cgctggtgct gatgggcgcg
     1981 gcgctggcaa tgatgaccga cgccggacgt agcgccatgc tcaaccctgg cccgcatggt
     2041 tttagcgaag tgctgtacgc cgtgtcatcc gccgctaaca acaacggcag cgcctttgcc
     2101 ggattaagcg ccaactcccc gttctggaac tgtttactgg cgttctgcat gtttgtcggt
     2161 cgcttcgggg tgattatccc ggtgatggca attgccggtt cgctggtgag taaaaagagc
     2221 caacccgcca gctccggcac gctgccaacg cacggcccgc tgtttgttgg cctgttaatc
     2281 ggcaccgtgt tgctggttgg cgcactgacc tttatccctg ccctggcgct tggtccggtg
     2341 gcggaatatc tctcctgatg atattgagtg agcactgaat atgagtcgta aacaactggc
     2401 gctattcgaa ccaacacttg tcgttcaggc gctgaaagaa gcggtgaaaa aattaaaccc
     2461 gcaggcgcaa tggcgcaatc cggtgatgtt tatcgtctgg atcggcagtc tgctgaccac
     2521 ctgtattagc atcgcgatgg caagcggtgc gatgcctggc aatgcgctgt ttagcgcggc
     2581 aattagcggc tggctgtggg tcaccgtact gttcgctaat ttcgccgagg cgctggcaga
     2641 aggccgcagt aaagcgcagg ccaacagtct taaaggggtg aaaaaaactg cctttgcccg
     2701 caagctgcgt gagccgaaat atggcgctgc ggcggacaaa gttcctgccg atcaactgcg
     2761 taaaggtgac atcgtgctgg tagaagctgg cgatattatc ccctgcgatg gtgaagtcat
     2821 tgaaggcggc gcatcggtcg atgaaagcgc catcaccggg gaatcggcac cggtgatccg
     2881 tgaatccggc ggcgattttg cctccgtcac cggcggcacg cgtattcttt ctgactggct
     2941 ggtgattgag tgtagcgtta accccggtga gacatttctg gatcggatga tcgcgatggt
     3001 ggaaggcgca cagcgacgca aaacaccgaa cgagattgcc ctgactatcc tgctgattgc
     3061 tctgaccatc gtctttttac tggcaaccgc cacgctgtgg ccgttttccg cgaggggcgg
     3121 taatgccgtg agtgtaacgg tactggtggc gctgctggtc tgtctgatcc caaccactat
     3181 tggcggcctg ttgtcagcga tcggcgtcgc cgggatgagt cggatgctgg gcgcgaatgt
     3241 gattgccacc agcggacgtg cagttgaagc ggcaggtgac gttgacgttc tgctactgga
     3301 taaaaccggc accatcacac tcggtaaccg tcaggcgtcg gagtttatcc ctgcgcaggg
     3361 cgtggatgaa aaaacgctgg ctgacgccgc acaactggct tcgctggctg atgaaacgcc
     3421 ggaaggccgc agtattgtga tcctcgccaa gcaacgtttt aacctgcgcg agcgcgatgt
     3481 gcagtcgctc catgccacct ttgtaccgtt tactgcgcaa agccggatga gcgggatcaa
     3541 catcgacaac cgcatgatcc gtaaaggttc tgtcgatgcc attcgccgcc atgttgaggc
     3601 taacggcggt cacttcccta ccgatgttga tcaaaaagtc gatcaggttg cgcgtcaggg
     3661 ggccacgccg ctggtggtgg tggaaggttc ccgtgtgctg ggcgttattg cgctgaaaga
     3721 tatcgtcaaa ggcggtatta aagagcgctt cgcccagctg cgcaaaatgg gcattaaaac
     3781 ggtgatgatt accggcgata accgtctgac tgccgccgcc attgctgcgg aagcgggcgt
     3841 cgatgatttt ctcgccgaag cgacaccgga ggccaagctg gcattgattc gtcagtatca
     3901 ggcggaaggc cgtttggtgg cgatgaccgg cgacggcacc aacgatgctc cagcgctggc
     3961 gcaggcagat gtcgcggtgg cgatgaactc cggtactcag gcggcgaaag aagcgggcaa
     4021 tatggtcgat ctcgactcta acccgaccaa gttgatcgag gtggtacata ttggcaaaca
     4081 gatgctgatg acccgtggct cgctgaccac cttcagcatt gccaacgatg tggcgaaata
     4141 cttcgccatt attccggcgg cgttcgcggc aacgtatccg cagttaaatg cgctgaacat
     4201 catgcgcctt cattcgcccg actccgccat tctcagtgcg gtcattttca acgccttgat
     4261 tatcgtcttt ttgattcctc tggcgttaaa aggcgtgagt tataaaccgc ttaccgcttc
     4321 tgccatgttg cgccgtaact tatggattta cggtctgggt gggctgctgg tgccgtttat
     4381 tggtatcaaa gtcattgatt tactgctgac cgtttgcggt ctggtgtgag gtttaccatg
     4441 cgtggcttac gtccggcatt atcgacattt atctttctgt tattgattac tggcggcgtt
     4501 tacccgctgc tgaccaccgt gctggggcaa tggtggtttc cctggcaggc caacggttcg
     4561 ttgattcgtg aaggtgatac ggtgcggggt tcggcattaa tcgggcagaa ttttaccggc
     4621 aacggctatt ttcagggtcg cccgtcggca acggcagaaa tgccctataa tccacaggct
     4681 tctggcggga gcaatctggc ggtcagtaac cctgagctgg ataaacaaat agccgcacgc
     4741 gttgctgcat tacgggccgc taacccggat gccagtacga acgttccggt tgaactggtg
     4801 acggcatcgg caagcgggct ggacaataat atcaccccgc aagcggcggt ctggcaaatc
     4861 ccacgcgtgg cgaaagcgcg taatctcagc gttgaacagc tcacgcaact gatcgcaaaa
     4921 tacagccaac aaccgctggt gaaatatatc ggccagccgg tcgtcaacat tgttgaactc
     4981 aatctggcgc tggataaact tgatgaataa cgaaccctta cgtcccgacc ccgatcgtct
     5041 gctggaacaa actgccgcgc cgcatcgggg gaagctgaaa gttttcttcg gtgcctgtgc
     5101 aggcgtcggg aagacctggg cgatgctggc agaagcccag cgactgcggg cgcaagggct
     5161 ggatattgtg gttggcgtgg tagaaaccca tgggcggaaa gataccgccg ccatgcttga
     5221 agggctggct gttctgccgc ccaaacgcca ggcgtaccgt gggcggcata tcagcgagtt
     5281 tgatctcgat gccgccctcg cccgccgtcc ggcgctgatc ttaatggacg aactggcgca
     5341 cagtaatgcg ccaggttctc gtcatcctaa acgctggcag gatatcgaag aactgctgga
     5401 agctggcatt gatgttttca caaccgtcaa cgttcagcat ctggaaagtc tgaatgatgt
     5461 ggtcagcggc gtcaccggaa ttcaggtacg ggaaaccgtg cccgatcctt ttttcgatgc
     5521 cgccgacgac gtagtgctgg tggacttgcc cccggacgat ctgcgccagc ggctgaaaga
     5581 aggcaaagtc tatattgccg ggcaggcgga gcgcgccatt gaacattttt tccgcaaagg
     5641 caatctgatc gccctgcgtg aactggcact gcgccgtact gccgatcgcg tagatgagca
     5701 aatgcgcgcc tggcgggggc atcctggtga agagaaagtg tggcacacgc gcgacgcgat
     5761 ccttttatgc atcgggcata acaccggcag tgagaaactg gtccgcgcgg cggcgcggtt
     5821 ggcctcgcgc ctgggtagcg tctggcacgc ggtgtatgtt gaaacccctg ccctgcatcg
     5881 cttaccggaa aaaaaacgtc gggcaattct cagcgcctta cgtctggcgc aggagctggg
     5941 cgcggagacg gctacactta ctgatccagc ggaagaaaaa gcagtagtgc gttatgcccg
     6001 tgaacataat ctcggcaaga ttattctcgg tcgcccggcc tcgtgccgct ggtggcgtcg
     6061 ggaaacgttt gctgaccgac tggcgcgcat cgcccccgat ctcgatcagg tgctggtcgc
     6121 gcttgatgaa ccacccgccc gcacgattaa caacgcgccg gatagccgct cttttaaaga
     6181 caagtggcgg gtacagattc agggatgcgt ggttgccgcc gcgttatgcg ccgttatcac
     6241 tttaattgcc atgcagtggc tgatggcgtt tgatgccgcc aacctggtga tgctgtatct
     6301 gcttggcgtg gtggtggtgg cgctatttta tggacgctgg ccttcggtgg ttgctaccgt
     6361 cattaatgtg gtgagtttcg atctcttttt tatcgcccca cgcggcacgc tcgctgtctc
     6421 tgatgtgcaa tatctgctga ccttcgcggt gatgttaacc gtcgggctgg tgatcgggaa
     6481 ccttactgcg ggtgtgcgtt atcaggcgcg ggtagcccgt taccgcgagc aacgcacacg
     6541 gcacttatat gaaatgtcga aagctctggc ggtgggccgc agtccgcagg acatcgccgc
     6601 caccagcgaa caatttattg cctccacgtt tcatgcccgc agtcaggtgt tgttgcccga
     6661 tgacaacggt aaattgcagc cgttaacaca tccgcaagga atgacgccat gggacgatgc
     6721 catcgcgcag tggagttttg ataaaggcct gcctgcgggt gcgggcaccg acacgttacc
     6781 cggcgtgccg taccagattt tgccgctaaa aagcggcgag aaaacctacg gactggtggt
     6841 ggtggaaccg gggaatctgc gtcagttgat gatcccggag cagcagcgcc tgctagagac
     6901 gtttacgctg ttagtcgcca atgcgtttga gcggctgacg ctgaccgcca gtgaagaaca
     6961 ggcgcggatg gcaagcgaac gtgaacagat ccgcaacgcc ctgctggcgg cgctttcgca
     7021 tgatttacgc acgccgctta cggtgctgtt tggtcaggca gaaatcttaa cgctcgatct
     7081 ggcaagcgaa ggatcgcccc acgcccgcca ggccagcgag attcgtcagc atgtgctgaa
     7141 cactacccga ctggtgaata atctactgga tatggcgcga attcagtccg gcggctttaa
     7201 tttgaagaaa gagtggttaa cgctggaaga agtagtcggc agcgctctgc aaatgctgga
     7261 accgggttta tcgtcgccca tcaatctttc tctgccagaa ccgctgacct taatccacgt
     7321 tgacgggcca ctctttgaac gggtgctgat taatctgctg gagaacgcgg tgaaatatgc
     7381 gggtgcgcag gccgaaattg gtatcgatgc ccacgttgag ggcgaaaatc tacaactgga
     7441 tgtctgggat aacggccccg gtcttccgcc aggccaggag cagacgatat ttgataagtt
     7501 tgctcgcggg aataaagagt cggcagtacc gggggtaggg cttggactgg caatttgtcg
     7561 ggcgatagtg gatgtacacg ggggcactat taccgcgttc aaccgaccgg aaggtggtgc
     7621 ctgttttcgt gttacacttc cccagcaaac cgctcctgaa cttgaagact ttcatgagga
     7681 tatgtgacaa acgttctgat tgttgaagat gaacaggcta ttcgtcgctt tctgcgcacg
     7741 gcgctggagg gcgacgggat gcgcgtctat gaggccgaaa cgctgcaacg cggcttgctg
     7801 gaagcggcta cccgtaagcc agatttgatt attctcgatc tcggcctgcc cgatggtgat
     7861 gggattgagt ttatccgcga cctgcgccag tggagcgcgg tgccggtgat tgtgctttcc
     7921 gcacgcagcg aagagagcga caaaatcgcc gcgctggatg ccggagcgga tgattatctg
     7981 agtaagccgt ttggcattgg cgaattgcag gcccgtctgc gcgtcgcttt acgccgccac
     8041 tccgccaccg ccgcgcccaa tccgctggtg aaattttccg atgttaccgt cgatttagcc
     8101 gcccgcgtga ttcaccgggg tgaggaagag gtgcatctca cgccgattga gttccgcctg
     8161 ctggcggtgc tgctaaataa tgccgggaaa gtgctcaccc agcgccagtt actcaatcag
     8221 gtgtgggggc caaacgcggt cgaacacagt cactatttgc gtatttatat gggacatctg
     8281 cgacaaaaac tggaacagga tcccgctcgc ccccgccatt tcattactga aaccggtatt
     8341 ggg
//
LOCUS       DAZKHF020000192         8329 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2201S31241
            SAMN43267800-rid25106413.denovo.193, whole genome shotgun sequence.
ACCESSION   DAZKHF020000192 DAZKHF020000000
VERSION     DAZKHF020000192.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267800
            Sequence Read Archive: SRR30307263
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 8329)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8329)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 8329)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350581.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350581.2
            Genome Coverage       :: 151x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:21:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,652
            CDSs (total)                      :: 5,557
            Genes (coding)                    :: 5,320
            CDSs (with protein)               :: 5,320
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 237
            CDSs (without protein)            :: 237
            Pseudo Genes (ambiguous residues) :: 0 of 237
            Pseudo Genes (frameshifted)       :: 102 of 237
            Pseudo Genes (incomplete)         :: 130 of 237
            Pseudo Genes (internal stop)      :: 52 of 237
            Pseudo Genes (multiple problems)  :: 41 of 237
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8329
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267800-rid25106413.denovo.193"
                     /serotype="O 26:H11"
                     /isolate="2201S31241"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-01"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            <1..277
                     /locus_tag="ACHOSC_RS17790"
                     /old_locus_tag="pgaptmp_003558"
     CDS             <1..277
                     /locus_tag="ACHOSC_RS17790"
                     /old_locus_tag="pgaptmp_003558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4549430.1"
                     /translation="ELTRKDNIFILFSSADDGPVMVVSSQRLHDMLNPTKDTNWNSTC
                     IYKSRHKMLPINLTQETLFSSKSHGKYALFPIFTASWRATRIKNIGI"
     gene            500..997
                     /gene="ubiC"
                     /locus_tag="ACHOSC_RS17795"
                     /old_locus_tag="pgaptmp_003559"
     CDS             500..997
                     /gene="ubiC"
                     /locus_tag="ACHOSC_RS17795"
                     /old_locus_tag="pgaptmp_003559"
                     /EC_number="4.1.3.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709881.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008813 - chorismate lyase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate lyase"
                     /protein_id="HGU4549431.1"
                     /translation="MSHPALTQLRALRYFKEIPALDPQLLDWLLLEDSMTKRFEQQGK
                     TVSVTMIREGFVEQNEIPEELPLLPKESRYWLREILLCADGEPWLAGRTVVPVSTLSG
                     PELALQKLGKTPLGRYLFTSSTLTRDFIEIGRDAGLWGRRSRLRLSGKPLLLTELFLP
                     ASPLY"
     gene            1010..1882
                     /gene="ubiA"
                     /locus_tag="ACHOSC_RS17800"
                     /old_locus_tag="pgaptmp_003560"
     CDS             1010..1882
                     /gene="ubiA"
                     /locus_tag="ACHOSC_RS17800"
                     /old_locus_tag="pgaptmp_003560"
                     /EC_number="2.5.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008412 - 4-hydroxybenzoate
                     octaprenyltransferase activity [Evidence IEA];
                     GO_process: GO:0006744 - ubiquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxybenzoate octaprenyltransferase"
                     /protein_id="HGU4549432.1"
                     /translation="MEWSLTQNKLLAFHRLMRTDKPIGALLLLWPTLWALWVATPGVP
                     QLWILAVFVAGVWLMRAAGCVVNDYADRKFDGHVKRTANRPLPSGAVTEKEARALFVV
                     LVLISFLLVLTLNTMTILLSIAALALAWVYPFMKRYTHLPQVVLGAAFGWSIPMAFAA
                     VSESVPLSCWLMFLANILWAVAYDTQYAMVDRDDDVKIGIKSTAILFGQYDKLIIGIL
                     QIGVLALMAIIGELNGLGWGYYWSIVVAGALFVYQQKLIANREREACFKAFMNNNYVG
                     LVLFLGLAMSYWHF"
     gene            complement(2037..4460)
                     /gene="plsB"
                     /locus_tag="ACHOSC_RS17805"
                     /old_locus_tag="pgaptmp_003561"
     CDS             complement(2037..4460)
                     /gene="plsB"
                     /locus_tag="ACHOSC_RS17805"
                     /old_locus_tag="pgaptmp_003561"
                     /EC_number="2.3.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313051.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004366 - glycerol-3-phosphate
                     O-acyltransferase activity [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate 1-O-acyltransferase PlsB"
                     /protein_id="HGU4549433.1"
                     /translation="MSGWPRIYYKLLNLPLSILVKSKSIPADPAPELGLDTSRPIMYV
                     LPYNSKADLLTLRAQCLAHDLPDPLEPLEIDGTLLPRYVFIHGGPRVFTYYTPKEESI
                     KLFHDYLDLHRSNPNLDVQMVPVSVMFGRAPGREKGEVNPPLRMLNGVQKFFAVLWLG
                     RDSFVRFSPSVSLRRMADEHGTDKTIAQKLARVARMHFARQRLAAVGPRLPARQDLFN
                     KLLASRAIAKAVEDEARSKKISHEKAQQNAIALMEEIAANFSYEMIRLTDRILGFTWN
                     RLYQGINVHNAERVRQLAHDGHELVYVPCHRSHMDYLLLSYVLYHQGLVPPHIAAGIN
                     LNFWPAGPIFRRLGAFFIRRTFKGNKLYSTVFREYLGELFSRGYSVEYFVEGGRSRTG
                     RLLDPKTGTLSMTIQAMLRGGTRPITLIPIYIGYEHVMEVGTYAKELRGATKEKESLP
                     QMLRGLSKLRNLGQGYVNFGEPMPLMTYLNQHVPDWRESIDPIEAVRPAWLTPTVNNI
                     AADLMVRINNAGAANAMNLCCTALLASRQRSLTREQLTEQLNCYLDLMRNVPYSTDST
                     VPSASASELIDHALQMNKFEVEKDTIGDIIILPREQAVLMTYYRNNIAHMLVLPSLMA
                     AIVTQHRHISRDVLMEHVNVLYPMLKAELFLRWDRDELPDVIDALANEMQRQGLITLQ
                     DDELHINPVHSRTLQLLAAGARETLQRYAITFWLLSANPSINRGTLEKESRTVAQRLS
                     VLHGINAPEFFDKAVFSSLVLTLRDEGYISDSGDAEPAETMKVYQLLAELITSDVRLT
                     IESATQGEG"
     gene            4631..4999
                     /gene="dgkA"
                     /locus_tag="ACHOSC_RS17810"
                     /old_locus_tag="pgaptmp_003562"
     CDS             4631..4999
                     /gene="dgkA"
                     /locus_tag="ACHOSC_RS17810"
                     /old_locus_tag="pgaptmp_003562"
                     /EC_number="2.7.1.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase"
                     /protein_id="HGU4549434.1"
                     /translation="MANNTTGFTRIIKAAGYSWKGLRAAWINEAAFRQEGVAVLLAVV
                     IACWLDVDAITRVLLISSVMLVMIVEILNSAIEAVVDRIGSEYHELSGRAKDMGSAAV
                     LIAIIVAVITWCILLWSHFG"
     gene            5109..5717
                     /gene="lexA"
                     /locus_tag="ACHOSC_RS17815"
                     /old_locus_tag="pgaptmp_003563"
     CDS             5109..5717
                     /gene="lexA"
                     /locus_tag="ACHOSC_RS17815"
                     /old_locus_tag="pgaptmp_003563"
                     /EC_number="3.4.21.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000646075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0009432 - SOS response [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor LexA"
                     /protein_id="HGU4549435.1"
                     /translation="MKALTARQQEVFDLIRDHISQTGMPPTRAEIAQRLGFRSPNAAE
                     EHLKALARKGVIEIVSGASRGIRLLQEEEEGLPLVGRVAAGEPLLAQQHIEGHYQVDP
                     SLFKPNADFLLRVSGMSMKDIGIMDGDLLAVHKTQDVRNGQVVVARIDDEVTVKRLKK
                     QGNKVELLPENSEFKPIVVDLRQQSFTIEGLAVGVIRNGDWL"
     gene            5790..7115
                     /gene="dinF"
                     /locus_tag="ACHOSC_RS17820"
                     /old_locus_tag="pgaptmp_003564"
     CDS             5790..7115
                     /gene="dinF"
                     /locus_tag="ACHOSC_RS17820"
                     /old_locus_tag="pgaptmp_003564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313054.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015297 - antiporter activity [Evidence
                     IEA];
                     GO_function: GO:0042910 - xenobiotic transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter DinF"
                     /protein_id="HGU4549436.1"
                     /translation="MAFLTSSDKALWHLALPMIFSNITVPLLGLVDTAVIGHLDSPVY
                     LGGVAVGATATSFLFMLLLFLRMSTTGLTAQAYGAKNPQALARALVQPLLLALGAGAL
                     IALLRTPIIDLALHIVGGSEAVLEQARRFLEIRWLSAPASLANLVLLGWLLGVQYARA
                     PVILLVVGNILNIVLDVWLVMGLHMNVQGAALATVIAEYATLLIGLLMVRKILKLRGI
                     SGEMLKTAWRGNFRRLLALNRDIMLRSLLLQLCFGAITVLGARLGSDIVAVNAVLMTL
                     LTFTAYALDGFAYAVEAHSGQAYGARDGSQLLDVWRAACRQSGIVALLFSVVYLLAGE
                     HIIALLTSLTQIQQLADRYLIWQVILPLVGVWCYLLDGMFIGATRAAEMRNSMAVAAA
                     GFALTLLTLPWLGNHGLWLALTVFLALRGLSLAAIWRRHWRNGTWFAAT"
     gene            7231..7440
                     /locus_tag="ACHOSC_RS17825"
                     /old_locus_tag="pgaptmp_003565"
     CDS             7231..7440
                     /locus_tag="ACHOSC_RS17825"
                     /old_locus_tag="pgaptmp_003565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CsbD family protein"
                     /protein_id="HGU4549437.1"
                     /translation="MNKDEAGGNWKQFKGKVKEQWGKLTDDDMTIIEGKRDQLVGKIQ
                     ERYGYQKDQAEKEVVDWETRNEYRW"
     gene            complement(7482..7997)
                     /gene="zur"
                     /locus_tag="ACHOSC_RS17830"
                     /old_locus_tag="pgaptmp_003566"
     CDS             complement(7482..7997)
                     /gene="zur"
                     /locus_tag="ACHOSC_RS17830"
                     /old_locus_tag="pgaptmp_003566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418470.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="zinc uptake transcriptional repressor Zur"
                     /protein_id="HGU4549438.1"
                     /translation="MEKTTTQELLAQAEKICAQRNVRLTPQRLEVLRLMSLQDGAISA
                     YDLLDLLREAEPQAKPPTVYRALDFLLEQGFVHKVESTNSYVLCHLFDQPTHTSAMFI
                     CDRCGAVKEECAEGVEDIMHTLAAKMGFALRHNVIEAHGLCAACVEVEACRHPEQCQH
                     DHSVQVKKKPR"
BASE COUNT         1904 a         2012 c         2273 g         2140 t
ORIGIN      
        1 tgaattaaca cgaaaggata atatctttat cctgttctcc tctgccgatg atgggccagt
       61 tatggtggta agctcccagc gcttacatga tatgttgaat cctacaaaag ataccaattg
      121 gaattccacg tgtatctata aatccagaca taagatgttg cctattaatc ttactcagga
      181 aacacttttt agctccaaat ctcatggtaa atatgcgctt ttcccaattt ttactgcgag
      241 ttggcgagct actcgtataa agaatatagg tatttaagta aaggaaaaca tcaccgttcc
      301 tggcatcatg ggcggtgatg cccctacggt tgccctcgcc agcacgggca tcggcaaagc
      361 gtaaggttca acagcggttt accacttcat gcgattgttg cgtttttgtt gcgcattaga
      421 tcacttaatt tgctttacat ctcccgtaaa cacttttctg cgatacaatg cctttacgtt
      481 atgtaacgga gagttcggca tgtcacaccc cgcgttaacg caactgcgtg cgctgcgcta
      541 ttttaaagag atccctgccc tggatccgca actgctcgac tggctgttgc tggaggattc
      601 catgacaaaa cgttttgaac agcagggaaa aacggtaagc gtgacgatga tccgcgaagg
      661 gtttgtcgag cagaatgaaa tccccgaaga actgccgctg ctgccgaaag agtctcgtta
      721 ctggttacgt gaaattttgt tatgtgccga tggtgaaccg tggcttgccg gtcgtaccgt
      781 cgttcctgtg tcaacgttaa gcgggccgga gctggcgtta caaaaattgg gtaaaacgcc
      841 gttaggacgc tatctgttca catcatcgac attaacccgg gactttattg agataggccg
      901 tgatgccgga ctgtgggggc gacgttcccg cctgcgatta agcggtaaac cgctgttgct
      961 aacagaactg tttttaccgg cgtcaccgtt gtactaagag gaaaaaaata tggagtggag
     1021 tctgacgcag aataagctgc tggcgtttca tcgcttaatg cgtacggata agccaattgg
     1081 cgcgttactg ctgctctggc caacattatg ggcgttgtgg gtggcgacac cgggcgttcc
     1141 tcagctctgg atcctggcgg tatttgtcgc gggtgtctgg ctgatgcgcg ctgctggatg
     1201 tgtggtgaat gattatgctg accgaaagtt tgatggtcat gttaagcgca cggcgaaccg
     1261 accacttccc agcggcgcgg taacagagaa agaggcgcgc gcactgtttg tagtgctggt
     1321 actgatttcg tttttactgg tactgacgct gaatacgatg accattctgt tgtcgattgc
     1381 cgctctggcg ttggcgtggg tgtacccgtt tatgaagcgg tatacccatc tcccgcaagt
     1441 ggtgctgggc gcggcgtttg gctggtcgat tccaatggct tttgccgctg tgagtgagtc
     1501 ggtgccattg agttgctggt taatgttcct cgccaatatt ctctgggcgg tggcttacga
     1561 cacgcagtat gcgatggttg accgcgatga tgatgtgaag attggcatta aatccacggc
     1621 aatcctgttc ggccaatacg ataaattgat tattggtatt ttgcagattg gcgtactggc
     1681 actgatggcg atcatcggtg agttaaatgg cttaggctgg ggatattact ggtcaattgt
     1741 ggtggctggc gcgctgtttg tttatcaaca aaaactgatt gccaaccgcg agcgtgaagc
     1801 ctgctttaaa gcatttatga ataataacta tgttggtctg gtactatttt tagggctggc
     1861 aatgagttac tggcatttct gatgatgtaa aaaagccgga tgatcatccg gctttcttct
     1921 gggttgcctg atgcgcggcg cttctcaggc ctacacaaca catcgcaatt tattgaattt
     1981 gcagattatg gaaggccgga taaggcgttt tcgccgcatc cggcaattct ctctgattac
     2041 ccttcgccct gcgtcgcact ctcaatcgtc aaacgcacgt ctgatgtaat caactccgcc
     2101 agcaactgat aaaccttcat cgtttctgcc ggttcggcat cgccgctatc gctgatatac
     2161 ccttcatcac gcagtgtcag caccagagaa ctgaacaccg ccttgtcgaa gaactccggc
     2221 gcgttgatgc cgtgcagcac ggagagacgt tgcgcgacgg tgcggctctc tttctccagc
     2281 gtaccgcggt tgatcgacgg gttggcactc aacaaccaga aggtgatggc ataacgttgc
     2341 agcgtttcgc gcgcgcctgc ggcgagcagc tgtagcgtgc gagaatgcac cgggttgata
     2401 tgcaactcat catcttgcag ggtaatcagc ccctgacgtt gcatctcatt tgccagcgca
     2461 tcaataacgt ccggcaactc gtcgcgatcc cagcgcagga acagctccgc tttcagcatt
     2521 gggtaaagca cattgacgtg ctccatcaat acgtcgcggg agatgtggcg atgctgggtg
     2581 acaattgccg ccatcagcga aggcagcacc aacatatgcg caatgttgtt gcgatagtag
     2641 gtcatcagca ccgcttgctc gcgtggcaga atgatgatgt cgccgatagt gtctttctcg
     2701 acttcaaact tgttcatttg cagcgcgtga tcgataagct cgctggcgct ggctgaagga
     2761 acggtagagt ccgtggagta aggtacgttg cgcatcagat ccaggtagca gttgagttgc
     2821 tcggttaact gctcgcgggt gagtgagcgc tgacgtgatg ccagtagcgc agtacagcac
     2881 aggttcatgg cgtttgccgc gcctgcgttg ttaatgcgta ccatcagatc ggcagcaata
     2941 ttattgaccg tcggcgttaa ccaggccgga cgcaccgctt cgatgggatc gatagattca
     3001 cgccagtctg gtacgtgctg gttaaggtag gtcatcaacg gcattggttc accgaagttg
     3061 acgtaaccct gaccgagatt acgcagcttg cttaaaccgc gcagcatctg cggcaggctc
     3121 tctttctctt tcgtcgcgcc gcgcagttct ttggcgtaag tacccacttc catgacgtgc
     3181 tcataaccga tatagatcgg aatcagcgta atcggacgcg tgccgccacg cagcatcgcc
     3241 tgaatggtca tcgacagcgt accggttttc ggatccagca aacgccccgt gcgggaacga
     3301 ccgccttcca cgaagtactc gacggaataa ccacggctga acagttcgcc gagatactca
     3361 cggaaaacgg tggaataaag tttattgcct ttaaaagtac ggcgaataaa gaacgccccc
     3421 agacggcgga aaatcggccc ggccggccag aagttcaggt tgatcccggc ggcgatatgc
     3481 ggcggcacca gcccctggtg gtacagcacg taagaaagta gcaggtagtc catgtgactg
     3541 cggtggcaag gcacatacac cagctcatga ccgtcgtggg ccagctggcg aacgcgctcg
     3601 gcgttatgga cgttgatgcc ctgataaagt cggttccagg tgaagcccag aatgcggtca
     3661 gtcaggcgga tcatctcgta agagaaattc gccgcaatct cttccatcag cgcaatcgcg
     3721 ttctgctgcg ctttttcatg ggagattttt ttgctgcgcg cttcatcttc taccgctttg
     3781 gcaatggcgc gggaggcgag cagcttatta aacagatcct gacgagcagg cagacgtggg
     3841 cccacagcag ccagacgttg acgggcaaag tgcatacgcg ccacgcgcgc cagtttctga
     3901 gcgatagttt tatctgtgcc gtgttcatcg gccatgcggc gcagagaaac tgacggcgag
     3961 aaacgcacaa aactgtcgcg accgagccac agtacggcga aaaatttttg tacgccgtta
     4021 agcatacgca gcggcgggtt tacttcgcct ttttcacgcc ccggcgcgcg accaaacatc
     4081 accgatactg gcaccatctg cacatccaga tttgggttgc tgcggtgcaa atcaagatag
     4141 tcgtggaaca gcttaataga ctcttctttc ggcgtgtaat aggtgaacac acgcggcccg
     4201 ccgtgaatga acacatagcg cggcagtagc gtgccgtcga tttccagcgg ctctaacggg
     4261 tcaggcaagt catgagccag acactgggcg cgcaacgtca gcaaatctgc tttcgagttg
     4321 tacggtaaaa cgtacataat tggacgagag gtatccagcc ccagttccgg ggcaggatct
     4381 gccggaatag acttgctttt taccaggatg cttaatggta aattcagtaa tttgtagtaa
     4441 attcgtggcc agccggacat aaacgatgta aagcctctgg ttaataatgc aaatgcgcgg
     4501 caaggatagc agaaagtcat gggaaattct gtggtatccg ctcatgtttc gcgcggcgtt
     4561 acgcaaaccc gaatcatcgg atttaacggt acactgatat tgacgctcat aatgtaaaaa
     4621 ggttctttca atggccaata ataccactgg attcacccga attatcaaag ctgctggcta
     4681 ttcctggaaa ggtttacgcg ctgcatggat caacgaagcg gcattccgtc aggaaggcgt
     4741 agcggtattg ttggcggtgg tcatcgcctg ctggctggat gtggacgcaa ttacccgcgt
     4801 gctgcttatc agctccgtga tgctggtgat gattgtggaa atcctcaata gcgccatcga
     4861 agcagtggtt gaccgaattg gctctgaata ccatgagctt tccggacgcg caaaagatat
     4921 gggatccgcc gcggtgctga ttgccattat cgtcgccgtg attacctggt gcatcctgtt
     4981 atggtcgcat tttggataac ccttccagat ttcgataaat ctctggttta ttgtgcagtt
     5041 tatggttcca aaatcgcctt ttgctgtata tactcacagc ataactgtat atacacccag
     5101 ggggcggaat gaaagcgtta acggccaggc aacaagaggt gtttgatctc atccgtgatc
     5161 acatcagcca gacaggtatg ccgccgacgc gtgcggaaat cgcgcagcgt ttggggttcc
     5221 gttccccaaa cgcggctgaa gaacatctga aggcgctggc acgcaaaggc gttattgaaa
     5281 ttgtttccgg cgcatcacgc gggattcgtc tgttgcagga agaggaagaa gggttgccgc
     5341 tggtaggtcg tgtggctgcc ggtgaaccgc ttctggcgca acagcatatt gaaggtcatt
     5401 atcaggtcga tccttccttg ttcaagccga atgctgattt cctgctgcgc gtcagcggga
     5461 tgtcgatgaa agatatcggc attatggatg gtgacttgct ggcagtgcat aaaactcagg
     5521 atgtacgtaa cggtcaggtc gttgtcgcac gtattgatga cgaagttacc gttaagcgcc
     5581 tgaaaaaaca gggcaataaa gtcgaactgt tgccagaaaa tagcgagttt aaaccaattg
     5641 tcgttgacct tcgtcagcag agcttcacca ttgaagggct ggcggttggg gttattcgca
     5701 acggcgactg gctgtaacat atctctgaga ccgcgatgcc gccaggcgtc gcggtttgtt
     5761 tttcatctct cttcatcagg cttgtctgca tggcattcct cacttcatct gataaagcac
     5821 tctggcatct cgccttaccc atgattttct ccaatatcac cgttccgttg ctgggactgg
     5881 tcgatacggc ggtaattggt catcttgata gcccggttta tttgggcggc gtggcggttg
     5941 gtgcaacggc gaccagcttt ctctttatgc tgttgctgtt tttacgcatg agcaccaccg
     6001 ggctgactgc gcaggcttat ggtgccaaaa atcctcaggc attagcccgt gcgctggtgc
     6061 aaccgttgct gttggcgttg ggggctgggg cgttaattgc gctgctgcgt acaccgatta
     6121 tcgatctggc gctgcatatt gttggcggta gtgaagcggt cctggaacag gcgcggcgct
     6181 ttcttgaaat ccgctggtta agcgctccgg cgtcgctggc gaatctggta ttactcggtt
     6241 ggttactcgg cgtgcaatat gcccgtgcgc cagtaatttt gttagtggtc ggcaatatcc
     6301 tcaacattgt gctggatgtc tggctggtga tggggctgca tatgaacgtg cagggcgcgg
     6361 cgctggcgac ggttattgcg gaatatgcaa cattgctgat tggtctgcta atggtgcgta
     6421 aaatcctcaa actacgcgga atttccggcg aaatgctgaa aactgcctgg cgaggaaact
     6481 tccgtcgctt gctggcgctt aaccgcgata tcatgctgcg ttcgctgttg ttgcaactct
     6541 gtttcggcgc gataaccgta cttggtgcgc gactggggag tgacattgtc gctgttaacg
     6601 cggttctgat gacgctactc acctttaccg cctatgcgct tgatggtttt gcctacgcgg
     6661 ttgaagcgca ctccggtcag gcatacggtg cgcgcgacgg tagccagttg ctggatgtat
     6721 ggcgggcagc gtgccgccag tcggggattg tagcgttact gttttcggtg gtttatttgc
     6781 tggcagggga acacatcatt gcgttgctga cgtcgttaac ccagattcag cagctggctg
     6841 accgctatct tatctggcag gtgattttgc cgttggttgg tgtctggtgt tatctgctgg
     6901 acggcatgtt tataggcgca acgcgtgccg ccgaaatgcg taacagtatg gcggtggccg
     6961 ccgcaggttt tgcgctgacg ctccttacgc tgccgtggct gggtaatcat ggtttgtggc
     7021 tggcattaac cgtctttctg gcgttgcgcg ggctttctct ggcggctatc tggcggcgtc
     7081 actggcgcaa cggtacctgg tttgccgcaa cgtgacggtt aaaaattctg aataaataat
     7141 cctaagccaa attgctgact acacttaatc tcacgttcag aagaaaagtg aacgtactct
     7201 cattcacaac ctaacgatga ggtcttgatt atgaataaag atgaagccgg cggtaactgg
     7261 aaacagttta aaggtaaagt gaaagagcaa tggggcaaac tgaccgatga tgatatgacg
     7321 atcattgaag gtaaacgtga tcaactggtc ggtaaaatcc aggaacgtta tggttatcag
     7381 aaagatcagg cagaaaaaga ggtcgtggat tgggaaaccc gcaatgaata tcgctggtaa
     7441 ttaatccctc ctgcccgacg tgtacaagga tgtacgccct cttaacgcgg tttctttttc
     7501 acctgcacag agtgatcatg ctggcactgt tcaggatgac gacacgcttc cacttccacg
     7561 catgccgcac agagcccatg tgcttcaatc acattatgcc gcagggcaaa ccccattttt
     7621 gccgccagcg tatgcataat gtcttccacg ccttctgcac actcttcttt caccgcgccg
     7681 cagcgatcgc aaataaacat ggctgacgta tgggtgggct ggtcgaacag atgacagagc
     7741 acataactgt tggtggattc caccttatgc acaaaacctt gctcaagcag aaaatccagc
     7801 gcgcgataaa ccgttggcgg cttggcttgc ggttcagctt cgcgcagtaa atcaagcaga
     7861 tcataagcgc tgatagcgcc atcctgcaga ctcatcaggc gcaacacttc caggcgctgt
     7921 ggggtcaggc gcacattacg ctgcgcgcag attttttcag cctgcgctaa taactcctgc
     7981 gttgtggtct tttccatcgg gcacctcaaa gtgggggtta agaatgtcct tactttacca
     8041 tgttccagga aaaacaccga gaacccttat tgttgccgta atgttgattt tctgttttgt
     8101 aggtaaggtg ttgtgttgcc ttgtcgtacc attatcaaca cgataataat taataattca
     8161 tttttaaata aattgcttca agttaactcg ctatttattt aactgggttt aaacgcatac
     8221 ctgtagttca gaaattatga gcgatttttg atagtgacca gatggcaaaa gaattataag
     8281 gaggacacgg tacgacaaaa aaggtagatg aatcatgttg aagataata
//
LOCUS       DAZKHF020000193         8293 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2201S31241
            SAMN43267800-rid25106413.denovo.194, whole genome shotgun sequence.
ACCESSION   DAZKHF020000193 DAZKHF020000000
VERSION     DAZKHF020000193.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267800
            Sequence Read Archive: SRR30307263
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 8293)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8293)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 8293)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350581.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350581.2
            Genome Coverage       :: 151x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:21:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,652
            CDSs (total)                      :: 5,557
            Genes (coding)                    :: 5,320
            CDSs (with protein)               :: 5,320
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 237
            CDSs (without protein)            :: 237
            Pseudo Genes (ambiguous residues) :: 0 of 237
            Pseudo Genes (frameshifted)       :: 102 of 237
            Pseudo Genes (incomplete)         :: 130 of 237
            Pseudo Genes (internal stop)      :: 52 of 237
            Pseudo Genes (multiple problems)  :: 41 of 237
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8293
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267800-rid25106413.denovo.194"
                     /serotype="O 26:H11"
                     /isolate="2201S31241"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-01"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            59..1036
                     /gene="glaH"
                     /locus_tag="ACHOSC_RS17835"
                     /old_locus_tag="pgaptmp_003567"
     CDS             59..1036
                     /gene="glaH"
                     /locus_tag="ACHOSC_RS17835"
                     /old_locus_tag="pgaptmp_003567"
                     /EC_number="1.14.11.64"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311547.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050498 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen, with 2-oxoglutarate as one donor, and
                     the other dehydrogenated [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutarate dioxygenase GlaH"
                     /protein_id="HGU4549439.1"
                     /translation="MNALTAVHNNAVDSGQDYSGFTLIPSAQSPRLLELTFTEQTTKQ
                     FLEQVAEWPVQALEYKSFLRFRVGKILDDLCANQLQPLLLKTLLNRAEGALLINAVGI
                     DDVAQADEMVKLATAVAHLIGRSNFDAMSGQYYARFVVKNVDNSDSYLRQPHRVMELH
                     NDGTYVEEITDYVLMMKIDEQNMQGGNSLLLHLDDWEHLDHYFRHPLARRPMRFAAPP
                     SKNVSKDVFHPVFDVDQQGRPVMRYIDQFVQPKDFEEGVWLSELSDAIETSKGILSVP
                     VPVGKFLLINNLFWLHGRDRFTPHPDLRRELMRQRGYFAYATHHYQTHQ"
     gene            1056..2324
                     /gene="lhgO"
                     /locus_tag="ACHOSC_RS17840"
                     /old_locus_tag="pgaptmp_003568"
     CDS             1056..2324
                     /gene="lhgO"
                     /locus_tag="ACHOSC_RS17840"
                     /old_locus_tag="pgaptmp_003568"
                     /EC_number="1.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417146.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-2-hydroxyglutarate oxidase"
                     /protein_id="HGU4549440.1"
                     /translation="MYDFVIIGGGIIGMSTAMQLIDVYPDARIALLEKESGPACHQTG
                     HNSGVIHAGVYYTPGSLKAQFCLAGNRATKAFCDQNDIRYDNCGKMLVATSDLEMERM
                     RALWERTAANGIEREWLNAEELREREPNITGLGGIFVPSSGIVSYREVTAAMAKIFQA
                     RGGEIIYNAEVSALSEHKNGVVVRTRQGGEYEASTLISCSGLMADRLVKMLGLEPGFI
                     ICPFRGEYFRLAPEHNQIVNHLIYPIPDPAMPFLGVHLTRMIDGSVTVGPNAVLAFKR
                     EGYRKRDFSFSDTLEILGSSGIRRVLQNHLRSGLGEMKNSLCKSGYLRLVQKYCPRLS
                     LSDLQPWPAGVRAQAVSPDGKLIDDFLFVTTPRTIHTCNAPSPAATSAIPIGAHIVSK
                     VQTLLASQSNPGRTLRAARSVDALHAAFNQ"
     gene            2347..3795
                     /gene="gabD"
                     /locus_tag="ACHOSC_RS17845"
                     /old_locus_tag="pgaptmp_003569"
     CDS             2347..3795
                     /gene="gabD"
                     /locus_tag="ACHOSC_RS17845"
                     /old_locus_tag="pgaptmp_003569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009013 - succinate-semialdehyde
                     dehydrogenase [NAD(P)+] activity [Evidence IEA];
                     GO_process: GO:0009450 - gamma-aminobutyric acid catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent succinate-semialdehyde
                     dehydrogenase"
                     /protein_id="HGU4549441.1"
                     /translation="MKLNDSNLFRQQALINGEWLDANNGEVIDVTNPANGDKLGSVPK
                     MGADETRAAIDAANRALPAWRALTAKERANILRNWFNLMMEHQDDLARLMTLEQGKPL
                     AEAKGEISYAASFIEWFAEEGKRIYGDTIPGHQADKRLIVIKQPIGVTAAITPWNFPA
                     AMITRKAGPALAAGCTMVLKPASQTPFSALALAELAIRAGIPAGVFNVVTGSAGAVGN
                     ELTSNPMVRKLSFTGSTEIGRQLMEQCAKDIKKVSLELGGNAPFIVFDDADLDKAVEG
                     ALASKFRNAGQTCVCANRLYVQDGVYDRFAEKLQQAVSKLHIGDGLDKGVTIGPLIDE
                     KAVAKVEEHIADALEKGARVVCGGKADERGGNFFQPTILVDVPANAKVSKEETFGPLA
                     PLFRFKDEADVIAQANDTEFGLAAYFYARDLSRVFRVGEALEYGIVGINTGIISNEVA
                     PFGGIKASGLGREGSKYGIEDYLEIKYMCIGL"
     gene            3809..5089
                     /gene="gabT"
                     /locus_tag="ACHOSC_RS17850"
                     /old_locus_tag="pgaptmp_003570"
     CDS             3809..5089
                     /gene="gabT"
                     /locus_tag="ACHOSC_RS17850"
                     /old_locus_tag="pgaptmp_003570"
                     /EC_number="2.6.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003867 - 4-aminobutyrate transaminase
                     activity [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009448 - gamma-aminobutyric acid metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-aminobutyrate--2-oxoglutarate transaminase"
                     /protein_id="HGU4549442.1"
                     /translation="MNSNKELMQRRSQAIPRGVGQIHPIFADRAENCRVWDVEGREYL
                     DFAGGIAVLNTGHLHPKVVAAVEAQLKKLSHTCFQVLAYEPYLELCEIMNQKVPGDFA
                     KKTLLVTTGSEAVENAVKIARAATKRSGTIAFSGAYHGRTHYTLALTGKVNPYSAGMG
                     LMPGHVYRALYPCPLHGISEDDAIASIHRIFKNDAAPEDIAAIVIEPVQGEGGFYAAS
                     PAFMQRLRALCDEHGIMLIADEVQSGAGRTGTLFAMEQMGVAPDLTTFAKSIAGGFPL
                     AGVTGRAEVMDAVAPGGLGGTYAGNPIACVAALEVLKVFEQENLLQKANDLGQKLKDG
                     LLAIAEKHPEIGDVRGLGAMIAIELFEDGDHSKPDAKLTAEIVARARDKGLILLSCGP
                     YYNVLRILVPLTIEDAQIRQGLEIISQCFAEAKQ"
     gene            5400..6800
                     /gene="gabP"
                     /locus_tag="ACHOSC_RS17855"
                     /old_locus_tag="pgaptmp_003571"
     CDS             5400..6800
                     /gene="gabP"
                     /locus_tag="ACHOSC_RS17855"
                     /old_locus_tag="pgaptmp_003571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015185 - gamma-aminobutyric acid
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GABA permease"
                     /protein_id="HGU4549443.1"
                     /translation="MGQSSQPHELGGGLKSRHVTMLSIAGVIGASLFVGSSVAIAEAG
                     PAVLLAYLFAGLLVVMIMRMLAEMAVATPDTGSFSTYADKAIGRWAGYTIGWLYWWFW
                     VLVIPLEANIAAMILHSWVPGIPIWLFSLVITLALTGSNLLSVKNYGEFEFWLALCKV
                     IAILAFIFLGAVAISGFYPYAEVSGISRLWDSGGFMPNGFGAVLSAMLITMFSFMGAE
                     IVTIAAAESDTPEKHIVRATNSVIWRISIFYLCSIFVVVALIPWNMPGLKAVGSYRSV
                     LELLNIPHAKLIMDCVILLSVTSCLNSALYTASRMLYSLSRRGDAPALMGKINRSKTP
                     YVAVLLSTGAAFLTVVVNYYAPAKVFKFLIDSSGAIALLVYLVIAVSQLRMRKILRAE
                     GSEIRLRMWLYPWLTWLVIGFITFVLVVMLFRPAQQLEVISTGLLAIGIICTVPIMAR
                     WKKLVLWQKTPVHNTR"
     gene            6821..7483
                     /gene="csiR"
                     /locus_tag="ACHOSC_RS17860"
                     /old_locus_tag="pgaptmp_003572"
     CDS             6821..7483
                     /gene="csiR"
                     /locus_tag="ACHOSC_RS17860"
                     /old_locus_tag="pgaptmp_003572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311552.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator CsiR"
                     /protein_id="HGU4549444.1"
                     /translation="MTITSLDGYRWLKNDIIRGNFQPDEKLRMSLLTSRYALGVGPLR
                     EALSQLVAERLVTVVNQKGYRVASMSEQELLDIFDARANMEAMLVSLAIARGGDEWEA
                     DVLAKAHLLSKLEACDASEKMLDEWDLRHQAFHTAIVAGCGSHYLLQMRERLFDLAAR
                     YRFIWLRRTVLSVEMLEDKHDQHQTLTAAVLARDTARASELMRQHLLTPIPIIQQAMA
                     GN"
     gene            complement(7484..7933)
                     /gene="kbp"
                     /locus_tag="ACHOSC_RS17865"
                     /old_locus_tag="pgaptmp_003573"
     CDS             complement(7484..7933)
                     /gene="kbp"
                     /locus_tag="ACHOSC_RS17865"
                     /old_locus_tag="pgaptmp_003573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="potassium binding protein Kbp"
                     /protein_id="HGU4549445.1"
                     /translation="MGLFNFVKDAGEKLWDAVTGQHDKDDQAKKVQEHLSKTGIPDAD
                     KVNIQIADGKATVTGDGLSQEAKEKILVAVGNISGIASVDDQVKTATPATASQFYTVK
                     SGDTLSAISKQVYGNANLYNKIFEANKPMLKSPDKIYPGQVLRIPEE"
     gene            complement(8017..8175)
                     /gene="yqaE"
                     /locus_tag="ACHOSC_RS17870"
                     /old_locus_tag="pgaptmp_003574"
     CDS             complement(8017..8175)
                     /gene="yqaE"
                     /locus_tag="ACHOSC_RS17870"
                     /old_locus_tag="pgaptmp_003574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YqaE/Pmp3 family membrane protein"
                     /protein_id="HGU4549446.1"
                     /translation="MGFWRIVITIILPPLGVLLGKGFGWAFIINILLTLLGYIPGLIH
                     AFWVQTRD"
BASE COUNT         1859 a         2184 c         2342 g         1908 t
ORIGIN      
        1 atggctacga aatgagcatc gccatgtcac cctacatctc ataagaggat cgcttctgat
       61 gaatgcactg accgccgtac acaataacgc tgtcgattca ggccaggact atagcggatt
      121 cactctcatc ccgtcggcgc aatccccgcg tctgctggaa ctcaccttca ccgaacagac
      181 gaccaaacag tttctcgaac aggttgccga atggcccgtg caggcgctgg agtacaaatc
      241 gtttctgcgt tttcgggtag gcaaaattct tgacgatctg tgtgcgaatc agctgcaacc
      301 attgctgttg aagaccctgc taaaccgtgc tgaaggtgcg ctgttgatca atgcggtggg
      361 tatcgatgat gtggcgcagg cagatgagat ggtgaagctg gcaacggcgg tagcgcatct
      421 gattggtcgc tcaaatttcg atgcgatgag cggtcagtat tacgcgcgtt tcgtggtgaa
      481 aaatgtcgat aactcagaca gctatctgcg tcagccgcac cgcgtgatgg aactgcacaa
      541 cgacggcacg tatgtcgaag agatcaccga ttacgtgctg atgatgaaaa tcgatgagca
      601 aaacatgcag ggtggaaatt cgttgctgct gcatctcgat gactgggaac atctggacca
      661 ctatttccgc catccactgg cgcgtcgccc gatgcgcttt gccgcgccgc cgagcaaaaa
      721 cgtcagcaaa gatgttttcc atccagtgtt cgacgttgat caacagggtc gcccggtgat
      781 gcgctatatc gaccagttcg tccagccaaa agattttgaa gaaggcgtgt ggttgagcga
      841 gctttccgat gccattgaaa ccagcaaagg cattctttct gtacccgttc ccgttggcaa
      901 attcctgttg ataaacaacc tgttctggct gcacggtcgc gaccgcttta ctccgcaccc
      961 ggatctgcgc cgtgaactga tgcgtcagcg tggctatttc gcttacgcca ctcaccacta
     1021 ccagacgcat cagtaagcgc aaaggaattg agcggatgta tgattttgtg attattggcg
     1081 gcggcatcat cggcatgtcg accgccatgc aactgattga tgtttatccg gatgcccgca
     1141 ttgcgttgct ggaaaaagag tccggcccgg cctgtcacca gacgggccac aacagcggcg
     1201 tgatccatgc cggggtctat tacacgcccg gcagcctgaa ggcgcagttt tgcctggcgg
     1261 gaaaccgcgc caccaaagcc ttttgcgatc aaaacgacat tcgctatgac aactgcggca
     1321 agatgctggt cgccacgtcc gatctcgaaa tggaacggat gcgcgcgttg tgggaacgca
     1381 cggcggcgaa cggtatcgag cgcgagtggt taaacgccga ggaactgcgc gagcgcgaac
     1441 cgaatatcac cgggctcggc ggcatttttg tgccgtccag cggcattgtc agctatcgcg
     1501 aagtcacagc ggcaatggca aaaatcttcc aggccagagg cggtgagatt atttataacg
     1561 ccgaagtcag cgccctcagt gagcataaaa acggcgtggt cgtccgaacc cgtcagggcg
     1621 gcgaatatga agcatcgacg ctgattagct gttccgggct gatggctgac cggctggtga
     1681 aaatgctcgg cctcgaaccg ggttttatta tctgcccgtt ccgtggcgag tatttccgcc
     1741 ttgcgccgga gcataaccag attgttaacc atctgattta ccccattccc gacccggcaa
     1801 tgccattttt gggcgtgcat ctcacccgaa tgatcgatgg cagcgtcacc gtcgggccaa
     1861 acgcggtgct ggctttcaaa cgcgaaggct atcgcaagcg cgacttctca tttagcgaca
     1921 cgctggagat tttgggctcg tcggggattc gccgggtgct gcaaaaccat ctacgctcag
     1981 gactgggcga gatgaaaaac tcgctgtgca aaagcggcta tctgcgcctg gtgcaaaagt
     2041 attgtccccg gctttcgtta agcgatctcc agccctggcc cgccggtgtg cgggcgcagg
     2101 cagtatcgcc ggacggcaag ctgattgacg attttctgtt tgtcaccacc ccacgcacga
     2161 tccacacctg caatgcgccc tccccggcag cgacatcagc aattcctatt ggtgcgcata
     2221 ttgtcagcaa ggtacaaacg ctgttggcaa gccagagtaa ccccggacgc acgctgcgag
     2281 cggcacgtag tgtggatgcc ttacacgccg catttaatca ataacctttg aaaacaggac
     2341 gtaacaatga aacttaacga cagtaactta ttccgccagc aggcgttgat taacggggaa
     2401 tggctggacg ccaacaatgg tgaagtcatc gacgtcacca atccggcgaa cggcgacaag
     2461 ctgggtagcg tgccgaaaat gggcgctgat gaaacccgcg ccgctatcga cgccgccaac
     2521 cgcgccctgc ccgcctggcg tgcgctcacc gccaaagaac gcgccaacat tctgcgcaac
     2581 tggttcaatt tgatgatgga gcatcaggac gatttagcgc gcctgatgac cctcgaacag
     2641 ggcaaaccac tggccgaagc gaaaggtgaa atcagctacg ccgcctcttt tattgagtgg
     2701 tttgccgaag aaggcaaacg catttatggc gacaccattc ccggtcatca ggccgataaa
     2761 cgcctgattg ttatcaagca gccgattggc gttaccgcag ctatcacgcc gtggaacttc
     2821 ccggcggcga tgattacccg taaagccggt ccggcgctgg cggcaggctg caccatggtg
     2881 ctgaagcccg ccagtcagac accgttctct gcgctggcgc tggcggagct ggcgattcgc
     2941 gcgggcattc cggctggcgt gtttaacgtg gtcaccggtt cggcaggcgc ggtcggtaat
     3001 gaactgacca gcaacccgat ggtacgcaaa ctgtcgttta ccggttcgac cgaaattggc
     3061 cgccagttaa tggaacagtg cgcgaaagac atcaaaaaag tgtcgctgga gctgggcggc
     3121 aacgcgccgt ttatcgtctt tgacgatgcc gatctcgata aagccgtgga aggcgcgctg
     3181 gcctcgaaat tccgcaacgc cgggcaaacc tgcgtctgcg ccaaccgttt atacgtgcag
     3241 gacggcgtgt atgaccgctt tgccgaaaaa ttgcagcagg cggtgagcaa actgcacatc
     3301 ggcgacgggc tggataaagg cgtcaccatc gggccgctga tcgatgaaaa agcggtagca
     3361 aaagtggaag agcatattgc cgatgcgctg gagaaaggtg cgcgtgtggt ttgcggcggt
     3421 aaagcggatg aacgcggcgg caacttcttc cagccgacca ttctggtgga cgtgccggcc
     3481 aacgccaaag tgtcgaaaga agagacgttc ggccccctcg ccccgctgtt ccgctttaaa
     3541 gatgaagccg atgtgattgc gcaagccaat gacaccgagt ttggccttgc cgcctatttc
     3601 tacgcccgtg atttaagccg cgtcttccgc gtgggcgaag cgctggagta cggcatcgtc
     3661 ggcatcaata ccggcattat ttccaatgaa gtggccccgt tcggcggcat caaagcctcg
     3721 ggtctgggtc gtgaaggttc gaagtatggc atcgaagatt acttagaaat caaatatatg
     3781 tgcatcggtc tttaactgga gaatgcgaat gaacagcaat aaagagttaa tgcagcgccg
     3841 cagtcaggcg attccccgtg gcgttgggca aattcacccg attttcgctg accgcgcgga
     3901 aaactgccgg gtgtgggacg ttgaaggccg tgagtatctt gatttcgcgg gcgggattgc
     3961 ggtgctcaat accgggcacc tgcatccgaa ggtggtggcc gcggtggaag cgcagttgaa
     4021 aaaactgtcg cacacctgct tccaggtgct ggcttacgag ccgtatctgg agctgtgcga
     4081 gattatgaat cagaaggtgc cgggcgattt cgccaagaaa acgctgctgg ttacgaccgg
     4141 ttccgaagcg gtggaaaacg cggtaaaaat cgcccgcgcc gccaccaaac gtagcggcac
     4201 catcgctttt agcggcgcgt atcacgggcg cacgcattac acgctggcgc tgaccggcaa
     4261 ggtgaatccg tactctgcgg gcatggggct gatgccgggt catgtttatc gcgcgcttta
     4321 tccttgcccg ctgcacggca taagcgagga tgacgctatc gccagcatcc accggatctt
     4381 caaaaatgat gccgcgccgg aagatatcgc cgccatcgtg attgagccgg ttcagggcga
     4441 aggcggtttc tacgccgcgt cgccagcctt tatgcagcgt ttacgcgcgc tgtgtgacga
     4501 gcacgggatc atgctgattg ccgatgaagt gcagagcggc gcggggcgta ccggcacgct
     4561 gtttgcgatg gagcagatgg gcgttgcgcc ggatctcact acctttgcga aatcgatcgc
     4621 gggcggcttc ccgctggcgg gcgtcaccgg gcgcgcggaa gtgatggatg ccgtcgctcc
     4681 aggcggtctg ggcggcacct atgccggtaa cccgattgcc tgcgtggcgg cgctggaagt
     4741 gttgaaggtg tttgagcagg aaaatctgct gcaaaaagcc aacgatctgg ggcagaagct
     4801 aaaagacgga ttgttggcga tagccgaaaa acacccggag atcggcgacg tacgcgggct
     4861 gggagcgatg attgccatcg agctgtttga agatggcgat cacagcaaac cggacgcaaa
     4921 actcaccgcc gagatcgtgg cacgcgcccg cgataagggc ctgattcttc tctcctgcgg
     4981 cccgtattac aacgtgctgc gcatccttgt accgctcacc attgaagacg ctcagatccg
     5041 tcagggtctg gagatcatca gccagtgttt tgctgaggcg aagcagtagc tatgtccgtt
     5101 tgtaagagtt aaacgttgct aagggaatga taacgatgca gtaaaaaata ataacgatga
     5161 ggcgatcgac aaagttaaaa acgactgcgg actgtcccct cgctcctctg gggagagggt
     5221 tagggtgagg ggtaatcacg cggctacacc ctcaccccgg ccctctccct aagggagagg
     5281 gagaaaactc tcccgctttc gtttttaaga ttttgttgac cgtctcaatt aaacgcgtga
     5341 cccgacgcgg gaaatgtcgg ggcgctctcc caagtgacac actttcgaga ggattcagga
     5401 tggggcaatc atcgcaacca catgagttag gcggcgggct gaagtcacgc cacgtcacca
     5461 tgttgtctat tgccggtgtt atcggcgcaa gtctgtttgt cggttccagc gtcgccatcg
     5521 ccgaagcggg cccggcggta ttactggcct atctgttcgc cggattactg gtggttatga
     5581 ttatgcggat gttggcggaa atggcagttg ccacgcccga taccggttcg ttttccacct
     5641 atgccgataa agccattggc cgctgggcgg gttataccat cggctggctg tactggtggt
     5701 tttgggtact ggttatcccg ctggaagcca acatcgccgc tatgatcctg cactcgtggg
     5761 ttccaggcat tcccatctgg ttattttccc tcgtcattac cctcgcctta actggcagta
     5821 atttattaag cgttaaaaac tacggcgaat ttgagttctg gctggcgctg tgcaaagtca
     5881 tcgctatcct ggcctttatt ttccttggtg cagtcgcaat tagcggtttt tacccttatg
     5941 ccgaagtgag cgggatctca agattgtggg atagcggcgg ctttatgccc aacggcttcg
     6001 gtgcggtatt aagcgcgatg ttgatcacca tgttctcgtt tatgggcgca gaaattgtca
     6061 ccattgccgc cgcggaatcc gacacgccgg aaaaacatat tgtccgcgct accaactcgg
     6121 ttatctggcg tatttctatc ttctatttgt gctctatttt tgtcgtagtg gcgttaatac
     6181 cgtggaatat gccggggctg aaagccgttg gttcttatcg ctcggttctg gaattgctca
     6241 atattcccca tgcgaaatta atcatggact gcgtgatatt actttccgta accagctgtc
     6301 tgaactcggc gctgtatacc gcgtcaagga tgctctactc cttaagccgt cgcggtgatg
     6361 cgcccgcgct aatgggcaaa atcaaccgca gtaaaacccc gtatgtggcg gtgttactct
     6421 ccaccggagc ggcattttta acggtggtgg tgaactatta cgcacctgcg aaagtgttta
     6481 aattcctgat agacagctcc ggcgctatcg ccctgctggt ttatttagtc atcgccgttt
     6541 cacagttgcg gatgcgcaaa attctgcgag cagaaggaag cgaaattcgc ttgcgcatgt
     6601 ggctttatcc ctggctcacc tggctggtca tcggctttat tacctttgtg ttggtagtga
     6661 tgctattccg tccggcgcaa cagttagaag tgatctccac cggcttatta gcgataggga
     6721 ttatctgtac cgtgccaatt atggctcgct ggaaaaagct ggtattgtgg caaaaaacac
     6781 ccgttcataa tacgcgctga taatgatcag gagtcacacc atgaccatta cgtctctgga
     6841 tggctatcgc tggctgaaga acgatattat tcgcggtaat tttcaaccgg atgaaaaatt
     6901 acggatgagt ttgctgacat cgcgttatgc acttggcgtt gggccgttgc gggaagcact
     6961 ttctcaactg gtggcggaac ggctggtcac ggtggtgaat caaaaagggt atcgggtggc
     7021 gtctatgtca gagcaggagc tgctcgatat tttcgacgcc cgcgccaata tggaagcgat
     7081 gctggtcagt ctggcgattg cccgcggtgg cgatgaatgg gaggcagacg ttctcgcaaa
     7141 agcgcatctg ctcagtaagc tggaagcctg tgacgccagc gagaaaatgc ttgatgagtg
     7201 ggatctgcgt catcaggcgt ttcatacagc aattgtggcg ggctgtggtt ctcactattt
     7261 gctgcaaatg cgtgaacggt tgtttgatct ggcggcgcgc tatcgattta tctggctgcg
     7321 gcgaacggtg ctttcggtag aaatgctgga ggataaacac gatcagcacc agaccctgac
     7381 tgcggcggta ctggcgcgag ataccgcgcg cgccagtgag ttaatgcgcc agcatttact
     7441 gacgccaatt cccattatcc agcaggcgat ggctggcaat taactactct tccggaatac
     7501 gcaacacttg ccccggataa attttatccg ggctttttag catcggttta ttcgcttcga
     7561 agattttatt gtacagatta gcgttaccgt agacctgttt ggaaatggca ctcagagtgt
     7621 cgccagactt aacggtataa aactggctgg cagtggctgg tgtcgccgtt ttcacctgat
     7681 catcgacact ggcaataccg gaaatattcc caaccgcaac aaggattttc tccttcgcct
     7741 cctgactcag gccgtcacca gtgaccgtcg ctttgccgtc ggcaatttga atgttcactt
     7801 tatcggcatc aggtataccg gttttgctca gatgctcctg caccttcttc gcctgatcgt
     7861 ctttatcgtg ctgacctgta accgcgtccc agagtttttc tccggcatct ttcacaaaat
     7921 tgaacagacc catagctacc tcattcgtta acggaaacac tcagaaagtg tagcagagga
     7981 acgcggcgcg ttaacgccgc gggggtgagc acaggtttaa tcgcgggttt gcacccagaa
     8041 cgcgtgaatc aaaccgggga tatacccaag cagtgtcagc agaatattaa taatgaacgc
     8101 ccaaccgaac cctttaccga gcagcacgcc gagcggcggc agaatgatgg tgatgacgat
     8161 tctccagaaa cccatatgta ctccctataa gaaaattact cattgtttaa aaagagattt
     8221 tatctcttaa gcgtagtaat tttagcggag cctgccagtt tcagcttacg cttttactct
     8281 catttacgct gct
//
LOCUS       DAZKHF020000194         8215 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2201S31241
            SAMN43267800-rid25106413.denovo.195, whole genome shotgun sequence.
ACCESSION   DAZKHF020000194 DAZKHF020000000
VERSION     DAZKHF020000194.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267800
            Sequence Read Archive: SRR30307263
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 8215)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8215)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 8215)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350581.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350581.2
            Genome Coverage       :: 151x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:21:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,652
            CDSs (total)                      :: 5,557
            Genes (coding)                    :: 5,320
            CDSs (with protein)               :: 5,320
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 237
            CDSs (without protein)            :: 237
            Pseudo Genes (ambiguous residues) :: 0 of 237
            Pseudo Genes (frameshifted)       :: 102 of 237
            Pseudo Genes (incomplete)         :: 130 of 237
            Pseudo Genes (internal stop)      :: 52 of 237
            Pseudo Genes (multiple problems)  :: 41 of 237
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8215
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267800-rid25106413.denovo.195"
                     /serotype="O 26:H11"
                     /isolate="2201S31241"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-01"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            complement(45..560)
                     /gene="ogt"
                     /locus_tag="ACHOSC_RS17875"
                     /old_locus_tag="pgaptmp_003575"
     CDS             complement(45..560)
                     /gene="ogt"
                     /locus_tag="ACHOSC_RS17875"
                     /old_locus_tag="pgaptmp_003575"
                     /EC_number="2.1.1.63"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003908 -
                     methylated-DNA-[protein]-cysteine S-methyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylated-DNA--[protein]-cysteine
                     S-methyltransferase"
                     /protein_id="HGU4549447.1"
                     /translation="MLRLLEEKIATPLGPLWVMCDEQFRLRAVEWEEYSERMVQLLDI
                     HYRKEGYERISATNPGGLSDKLRDYFAGNLSIIDTLPTATGGTPFQREVWKTLRTIPC
                     GQVMHYGQLAEQLGRPGAARAVGAANGSNPISIVVPCHRVIGRNGTMTGYAGGVQRKE
                     WLLRHEGYLLL"
     gene            complement(571..2097)
                     /gene="abgT"
                     /locus_tag="ACHOSC_RS17880"
                     /old_locus_tag="pgaptmp_003576"
     CDS             complement(571..2097)
                     /gene="abgT"
                     /locus_tag="ACHOSC_RS17880"
                     /old_locus_tag="pgaptmp_003576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415852.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="p-aminobenzoyl-glutamate transporter"
                     /protein_id="HGU4549448.1"
                     /translation="MSMSSIPSSSQSGKLYGWVERIGNKVPHPFLLFIYLIIVLMVTT
                     AILSAFGVSAKNPTDGTPVVVKNLLSVEGLHWFLPNVIKNFSGFAPLGAILALVLGAG
                     LAERVGLLPALMVKMASHVNARYASYMVLFIAFFSHISSDAALVIMPPMGALIFLAVG
                     RHPVAGLLAAIAGVGCGFTANLLIVTTDVLLSGISTEAAAAFNPQMHVSVIDNWYFMA
                     SSVVVLTIVGGLITDKIIEPRLGQWQGNSDEKLQTLTESQRFGLRIAGVVSLLFIAAI
                     ALMVIPENGILRDPINHTVMPSPFIKGIVPLIILFFFVVSLAYGIATRTIRRQADLPH
                     LMIEPMKEMAGFIVMVFPLAQFVAMFNWSNMGKFIAVGLTDILESSGLSGIPAFVGLA
                     LLSSFLCMFIASGSAIWSILAPIFVPMFMLLGFHPAFAQILFRIADSSVLPLAPVSPF
                     VPLFLGFLQRYKPDAKLGTYYSLVLPYPLIFLVVWLLMLLAWYLVGLPIGPGIYPRLS
                     "
     gene            complement(2134..3579)
                     /gene="abgB"
                     /locus_tag="ACHOSC_RS17885"
                     /old_locus_tag="pgaptmp_003577"
     CDS             complement(2134..3579)
                     /gene="abgB"
                     /locus_tag="ACHOSC_RS17885"
                     /old_locus_tag="pgaptmp_003577"
                     /EC_number="3.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="p-aminobenzoyl-glutamate hydrolase subunit AbgB"
                     /protein_id="HGU4549449.1"
                     /translation="MQEIYRFIDDAIEADRQRYTDIADQIWDHPETRFEEFWSAEHLA
                     SALESASFTVTRNVGNIPNAFIASFGQGKPVIALLGEYDALAGLSQQAGCAQPTSATP
                     GENGHGCGHNLLGTAAFAAAIAVKKWLEQYGQGGTVRFYGCPGEEGGSGKTFMVREGV
                     FDDVDAALTWHPEAFAGMFNTRTLANIQASWCFKGIAAHAANSPHLGRSALDAVTLMT
                     TGTNFLNEHIIEKARVHYAITNSGGISPNVVQAQAEVLYLIRAPEMTDVQHIYDRVAK
                     IAEGAALMTETTVECRFDKACSSYLPNRTLENAMYHALSHFGTPEWNSEELAFAKQIQ
                     ATLTPNDRQNSLNNIAATGGENGKAFALRHRETVLANEVAPYAATDNVLAASTDVGDV
                     SWKLPVAQCFSPCFAVGTPLHTWQLVSQGRTSIAHKGMLLAAKTMAATTVNLFIDSGL
                     LQECQQEHQQVTDTQPYHCPIPKNVTPSPLK"
     gene            complement(3579..4889)
                     /gene="abgA"
                     /locus_tag="ACHOSC_RS17890"
                     /old_locus_tag="pgaptmp_003578"
     CDS             complement(3579..4889)
                     /gene="abgA"
                     /locus_tag="ACHOSC_RS17890"
                     /old_locus_tag="pgaptmp_003578"
                     /EC_number="3.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415854.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="p-aminobenzoyl-glutamate hydrolase subunit AbgA"
                     /protein_id="HGU4549450.1"
                     /translation="MESLNQFVNSLAPKLSHWRRDFHHYAESGWVEFRTATLVAEELQ
                     QLGYSLALGREVVNESSRMGLPDELTLQREFERARQQGALAQWIAVFEGGFTGIVATL
                     DTGRPGPVMAFRVDMDALDLSEEQDVSHRPYRDGFASCNAGMMHACGHDGHTAIGLGL
                     AHTLKQFESGLHGVIKLIFQPAEEGTRGARAMVDAGVVDDVDYFTAVHIGTGVPAGTV
                     VCGSDNFMATTKFDAHFTGTAAHAGAKPEDGHNALLAAAQATLALHAIAPHSEGASRV
                     NVGVMQAGSGRNVVPASALLKVETRGASDVINQYVFDRAQQAIQGAATMYGVGVETRL
                     MGAATASSPSPQWVAWLQSQAAQVAGVNQAIERVEAPAGSEDATLMMARVQHHQGQAS
                     YVVFGTQLAAGHHNEKFDFDEQVLAIAVETLARTALNFPWTRGI"
     gene            5065..5973
                     /locus_tag="ACHOSC_RS17895"
                     /old_locus_tag="pgaptmp_003579"
     CDS             5065..5973
                     /locus_tag="ACHOSC_RS17895"
                     /old_locus_tag="pgaptmp_003579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HGU4549451.1"
                     /translation="MAFQVKIHQIRAFVEVARQGSIRGASRMLNMSQPALSKSIQELE
                     EGLAAQLFFRRSKGVTLTDAGESFYQHASLILEELRAAQEEIRQRQGQLAGQINIGMG
                     ASISRSLMPAVISRFHQQHPQVKVRIMEGQLVSMINELRQGELDFTINTYYQGPYDHE
                     FTFEKLLEKQFAIFCRPGHPAIGARSIKQLLDYSWTMPTPHGSYYKQLSELLDDQAQT
                     PQVGVVCETFSACISLVAKSDFLSILPEEMGCDPLHGQGLVMLPVSEILPKAAYYLIQ
                     RRDSRQTPLTASLITQFRRECGYLQS"
     gene            6303..6866
                     /gene="smrA"
                     /locus_tag="ACHOSC_RS17900"
                     /old_locus_tag="pgaptmp_003580"
     CDS             6303..6866
                     /gene="smrA"
                     /locus_tag="ACHOSC_RS17900"
                     /old_locus_tag="pgaptmp_003580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA endonuclease SmrA"
                     /protein_id="HGU4549452.1"
                     /translation="MNLDDKSLFLDAMEDVQPLKRATDVHWHPTRNQRAPQRIDTLQL
                     DNFLTTGFLDIIPLSQPLEFRREGLQHGVLDKLRSGKYPQQASLNLLRQPVEECRKMM
                     FSFIQQAMADGLRNVLIIHGKGRDDKSHANIVRSYVARWLTEFDDVQAYCTALPHHGG
                     SGACYVALRKTAQAKQENWERHAKRSR"
     gene            complement(6887..8119)
                     /gene="dgcM"
                     /locus_tag="ACHOSC_RS17905"
                     /old_locus_tag="pgaptmp_003581"
     CDS             complement(6887..8119)
                     /gene="dgcM"
                     /locus_tag="ACHOSC_RS17905"
                     /old_locus_tag="pgaptmp_003581"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309952.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase DgcM"
                     /protein_id="HGU4549453.1"
                     /translation="MITHNFNTLDLLTSPVWIVSPFEEQLIYANSAARLLMQDLTFSQ
                     LRTGPYSVSSQKELPKYLSDLQNQHDIIEILTVQRKEEETALSCRLVLRELTETEPVI
                     IFEGIEAPATLGLKASRSANYQRKKQGFYARFFLTNSAPMLLIDPSRDGQIVDANLAA
                     LNFYGYNHETMCQKHTWEINMLGRRVMPIMHEISHLPGGHKPLNFVHKLADGSTRHVQ
                     TYAGPIEIYGDKLMLCIVHDITEQKRLEEQLEHAAHHDAMTGLLNRRQFYHITEPGQM
                     QHLAIAQDYSLLLIDTDRFKHINDLYGHSKGDEVLCALARTLESCARKGDLVFRWGGE
                     EFVLLLPRTPLDTALSLAETIRVSVAKVSISGLPRFTVSIGVAHHEGNESIDELFKRV
                     DDALYRAKNDGRNRVLAA"
BASE COUNT         2089 a         2082 c         2065 g         1979 t
ORIGIN      
        1 ctttacataa aagtgtgaac aagctggcac aaattgttta atgtttacag caaaagataa
       61 ccttcgtggc gcaataacca ctcttttcgc tgaactccgc ctgcatatcc ggtcatggtg
      121 ccgtttcgac caataacccg atggcaaggt acgacgatgc tgatgggatt cgatccattt
      181 gccgcaccta cggcacgcgc cgcgccaggg cggcccaatt gttcagccag ttggccgtaa
      241 tgcattacct gcccgcaggg gatagtgcgt agagttttcc agacttcgcg ctgaaatggc
      301 gtcccccctg tggcagtggg aagcgtatca ataatgctaa gattaccggc aaaataatca
      361 cgaagcttgt cgcttaaacc gcctgggttg gtggcagaaa tgcgctcata gccttcttta
      421 cgataatgga tgtccagcag ctgcaccatg cgttcgctgt actcttccca ttcaaccgcc
      481 cgcaggcgaa attgctcatc gcacatcacc cacagtggac ccagtggcgt ggcaattttt
      541 tcttcaagta atctcagcat ccgttctctc ttaagacaaa cgtgggtaaa tccccggacc
      601 tatcggcaga ccaacaagat accacgccag caacatcagc agccatacca ccaaaaagat
      661 aagcggatag ggcaagacta acgaatagta agtacccagt ttcgcgtctg gtttgtagcg
      721 ttgcaggaat ccaagaaaca gtggaacaaa aggagatacc ggcgctaaag gcaatacgga
      781 tgagtcggca atacgaaaga ggatttgcgc aaatgccggg tgaaagccaa gtagcataaa
      841 cattggtacg aaaatggggg ccagaatcga ccagattgcg gaaccgctgg caataaacat
      901 gcataagaaa gaggaaagca acgccagacc gacaaacgcc gggatgccgc taagccctga
      961 actttcaagt atatcggtca gccccacggc gatgaatttc cccatgttgc tccagttaaa
     1021 catggcgaca aactgggcga ggggaaaaac catcacgata aatcccgcca tctctttcat
     1081 cggttcaatc attaaatgcg gtaaatccgc ctgacgtcga attgtgcggg tagcgatgcc
     1141 ataagccagc gaaaccacaa agaaaaaaag aatgattagt ggcacgatac ctttaataaa
     1201 gggtgatggc atcacggtgt gattaatcgg atcgcgcaat atcccgtttt ccgggatcac
     1261 catcagcgcg atcgcagcaa taaaaagtag cgatacgaca cctgctatgc gtaaaccaaa
     1321 acgctgactt tcggtcaatg tctgcagttt ctcatcgctg tttccctgcc attgacctaa
     1381 ccgtggctcg atgattttgt cggttatcaa gccgccaaca atcgtcagta cgactacgga
     1441 gctggccata aaataccagt tatcaattac actgacgtgc atttgcggat tgaacgcagc
     1501 cgccgcttcc gtgctgatcc ccgacagcaa cacgtcggtt gtgacaatca gtaaattagc
     1561 cgtaaagccg caacctacgc ctgcaatggc agccagtaaa cctgcaactg gatgcctgcc
     1621 caccgccaga aaaatcagcg cacccatcgg tggcatgatc actaacgccg catcggaaga
     1681 aatgtggctg aaaaaagcaa taaacagcac catataactg gcgtagcggg cattaacatg
     1741 cgatgccatt ttaaccatta gcgctggcag taagccgacg cgctccgcta ggccggcacc
     1801 taaaaccagc gccaggatcg ctccaagtgg agcaaaaccg ctaaagtttt taatcacatt
     1861 gggtaaaaac cagtgtaatc cttccacact gagcaggttt ttcaccacca ccggcgtacc
     1921 atcggtcggg tttttcgcac tgacgccaaa ggctgacaaa attgccgtcg tcaccatgag
     1981 tacgattatc aaatagataa agagcaaaaa aggatgggga accttgttac caattctttc
     2041 gacccagcca tagagcttcc cggattggga ggacgacggt atggatgaca tactcatggg
     2101 cattcctcgg ttgttgtgtt tgcgttgttg ttgttatttt aaaggtgacg gtgtcacgtt
     2161 tttcgggata gggcagtgat acggttgcgt gtccgtaact tgctgatgct cttgttggca
     2221 ttcttgcaat agccctgaat caatgaagag attgactgtg gttgctgcca tagttttcgc
     2281 cgccagcagc attcctttat gagcaataga tgttcgcccc tggctaacca gttgccacgt
     2341 atgtagcggt gtaccgacgg caaaacaggg gctgaaacac tgggcaacag gcagtttcca
     2401 actgacgtcg ccgacatcag tcgatgccgc aagcacgtta tcggtggcgg catatggagc
     2461 gacctcattc gccagtaccg tttcacgatg acgtagtgca aaagccttgc cgttttcgcc
     2521 accggttgcg gcgatattat tcagactgtt ttgccgatcg ttgggggtga gcgtagcctg
     2581 aatttgtttc gcaaaagcca gttcttcgga gttccattcc ggggtaccaa aatgggatag
     2641 ggcatggtac atggcatttt ctaaggtgcg attcgggaga taactggaac aggctttgtc
     2701 gaagcggcat tcaaccgtgg tttcggtcat caatgccgca ccttcggcga ttttggcgac
     2761 ccgatcataa atatgctgca cgtcggtcat ttcgggggcg cggataagat aaagcacttc
     2821 tgcctgcgcc tggaccacgt tgggcgagat cccgccgcta tttgtgatgg catagtgtac
     2881 gcgcgctttt tcaataatat gttcgttgag gaagttggtg ccagtggtca tcaacgttac
     2941 ggcatcaagg gcgctgcgtc ccaaatgagg ggaattcgcg gcatgtgctg cgatcccttt
     3001 aaagcaccat gatgcctgaa tgtttgccag cgtgcgggta ttgaacatgc cggcaaaggc
     3061 ttccgggtgc caggtgagtg ccgcatccac atcatcaaat accccctcgc ggaccatgaa
     3121 cgttttaccc gagccgcctt cttcgccagg acaaccataa aagcgcactg tgccgccttg
     3181 cccatattgt tccagccatt tcttgacggc tattgcagcg gcaaaggcgg cggttcccag
     3241 caaattgtgt ccgcaaccgt gaccattttc accgggcgtc gcggatgtag gttgcgcgca
     3301 accagcttgc tgacttaaac ctgccagggc gtcatattct cccagcaggg cgataaccgg
     3361 tttgccttga ccaaacgaag caataaatgc atttgggata ttgcctacgt tgcgggtaac
     3421 ggtgaagctt gcagattcca gcgccgaagc cagatgctcc gctgaccaga actcttcaaa
     3481 acgtgtttct ggatgatccc agatttgatc ggcaatatcg gtataacgtt ggcgatcggc
     3541 ttcaatcgca tcgtcgataa aacgatagat ttcctgcatc agatacctcg cgtccaggga
     3601 aaattgagcg cggtgcgcgc cagcgtttcg acggcaatag cgagaacctg ctcgtcaaaa
     3661 tcgaattttt cgttgtgatg acctgccgcc agctgtgtgc caaacaccac gtaggaggct
     3721 tgcccttgat ggtgctgcac gcgggccatc attaatgtgg catcttcgga acccgcaggc
     3781 gcttcaacac gttcaatggc ctgattgacc cccgcgacct gagccgcctg actttgcaac
     3841 catgcgaccc attgcggcga aggagaactg gcggtagctg cacccatcag gcgagtttca
     3901 acgccgacac catacatggt tgctgcgccc tgaatcgctt gttgtgcacg gtcaaaaaca
     3961 tattgattaa tgacgtcgct ggccccgcgt gtttccactt tcagcaacgc cgaggcagga
     4021 acaacgttac ggccgcttcc tgcctgcata acgcccacgt ttactctgga agctccttcg
     4081 ctgtgcgggg cgattgcatg cagtgcaaga gttgcttgtg ctgccgccaa caaggcattg
     4141 tgaccgtctt ctggttttgc gcctgcgtga gcggcggtcc cggtgaagtg cgcgtcaaat
     4201 ttggtggttg ccataaaatt atcactgccg cacaccacgg tgcccgcagg tacgccagtg
     4261 ccaatgtgca cggcagtaaa ataatcaaca tcatctacga cacctgcatc gaccatcgcc
     4321 cgcgcgccac gcgtaccttc ctctgcaggc tgaaaaatca gtttgatgac gccatgtagt
     4381 ccggactcga attgtttaag ggtatgcgcc agcccaagcc caatggcggt atgcccatca
     4441 tgaccacagg catgcatcat tccggcgtta catgacgcaa aaccgtcgcg gtaggggcga
     4501 tggctgacat cctgctcttc actgagatcc agcgcgtcca tatcgacacg gaaagccata
     4561 accggaccgg ggcgaccggt atccagggtg gcgacgatac ccgtgaaacc accttcaaaa
     4621 accgcaatcc attgtgctag cgcaccctgt tgacgagcgc gctcgaattc gcgttgtaga
     4681 gtgagttcat caggtagtcc catccggcta ctttcattaa ctacttcgcg gcccagcgcc
     4741 agtgaatagc cgagctgctg caattcttcc gcaacaaggg tggcagtgcg gaattccacc
     4801 cagccagact ctgcatagtg atgaaaatca cgtcgccagt gcgataattt tggggcaagc
     4861 gaattaacaa attgattcaa agactccata acctttcccg tcatcagtaa aaagtgtgac
     4921 ccggttcacg tagcgatagt ttttacttat cactaactga tttttcacag ttttaaccgt
     4981 tcataaatta ccctgacaca atcatctgca ttaaagtaga tgccagtttc tttggtctga
     5041 taaataacgg ttatcggtgg cgtcatggct tttcaggtaa aaattcatca aattcgggct
     5101 tttgttgaag tggctcgtca gggcagcatt cgcggagcga gccgaatgtt gaatatgtcg
     5161 caaccggcac tgagtaaatc tattcaggag ctggaagaag gattagcggc gcaactcttt
     5221 tttcgccgta gtaaaggcgt gacgttaact gatgccggtg aaagttttta tcagcacgcc
     5281 agtcttattc ttgaagagct gcgcgcagcg caagaggaaa ttcgccaacg acaagggcaa
     5341 ctggcagggc agattaatat cggcatgggg gccagtattt cccgcagtct gatgccagct
     5401 gtcatatctc gttttcatca gcagcatccg caggtaaaag tacgcattat ggaagggcaa
     5461 ctggtgtcga tgattaatga attgcgtcag ggagaattgg atttcaccat caatacctat
     5521 tatcagggac cgtacgacca cgaatttact tttgagaaat tactggaaaa gcaattcgcg
     5581 atcttttgcc gcccgggaca ccccgccatt ggtgcccgtt cgatcaaaca gttactggat
     5641 tacagctgga caatgccgac gccacacggc agttactaca aacagttgag tgaattgctt
     5701 gacgatcagg cgcaaacgcc acaggtcggt gtagtctgcg agacgttctc agcctgtatc
     5761 agtctggtgg caaaaagcga ttttctcagc atactgcctg aagaaatggg ctgcgatccc
     5821 ttgcacggac aggggctggt gatgttgccg gttagcgaaa ttttaccgaa agcggcctat
     5881 tatttgattc agcggcgtga tagtcgccag acaccactga ccgcgtcatt aatcacgcaa
     5941 ttccggcgag aatgcggcta tctgcaaagt taaaactgca taaaaaaata gagtctgtcg
     6001 acatccgcca gactctacag tacacacagc agtgcatccg cgtcttaagt ccggcattgt
     6061 ctcctctgcg ccggtgactg tgttataacg gttttaacct ttagttgcca attttcttca
     6121 gtgacagatt tcacgaaaat ataagttaca ttattaatat cgtgaatgaa taatcatgca
     6181 taagcatttt gcttaaaata tcggcaatat ttggaactta ttactggaat tttgggtaag
     6241 acgttgttgg accgacccgg tctggttatc atatcgcgct cttaattgcg ggaggatgta
     6301 acatgaacct tgacgacaaa tcgctgtttc ttgacgccat ggaagatgtc cagccgctga
     6361 aacgtgctac cgatgtccac tggcatccaa cgcgtaacca acgtgcgccg cagcgtatcg
     6421 acacgctgca gcttgataat ttcctcacca ccggatttct cgacatcatc ccactaagtc
     6481 agccgctgga gtttcggcgg gaagggttgc aacatggggt gctggataag ctgcgcagtg
     6541 gtaaatatcc gcaacaggcg agcctgaatc ttttgcgcca gccggtggaa gagtgccgca
     6601 aaatgatgtt cagtttcatt caacaagcca tggctgatgg tttgcgtaac gtgctgatta
     6661 ttcatggcaa aggacgggat gataaatcgc atgccaatat tgtccgcagc tatgtggcgc
     6721 gctggctgac cgaatttgat gatgttcagg catattgcac cgcgctaccg catcatggcg
     6781 gcagcggggc gtgttacgtc gcactacgta aaacggcgca ggcgaagcaa gaaaactggg
     6841 agcgccacgc taagcgcagc cgttgatctc gagacgcatc cgcggcttat gccgccagca
     6901 cgcggttgcg tccatcattt ttcgcccgat acaaagcatc atcaacgcgt ttaaacagtt
     6961 catcgatgct ttcatttcct tcgtgatgcg ccacaccaat gctgacggta aaacgtggta
     7021 agcccgaaat actcactttt gccacgctta cacggatagt ttcagccagc gaaagcgcgg
     7081 tatccagtgg ggttcttggt agcaataaga caaactcttc gcctccccaa cgaaacacca
     7141 aatcgccttt gcgagcgcaa ctttcgaggg tgcgggcgag ggcgcataac acctcatcac
     7201 ctttagaatg cccatagaga tcgttaatgt gtttaaaacg atcggtgtcg atgagcaaca
     7261 agctgtaatc ctgggcgatg gcgaggtgct gcatttggcc tggctcggta atgtgataaa
     7321 actgtcgccg attcagtaat ccggtcatcg cgtcatggtg agcagcatgt tccagctgct
     7381 cctccagccg tttttgctca gtaatatcat gcacaataca taacatgagc ttgtcgccat
     7441 aaatttcaat cggcccggca taggtctgca catgacgagt agatccatcc gccagtttat
     7501 gaacaaaatt caaaggttta tgaccgccgg gtaaatgcga aatttcatgc atgataggca
     7561 tgacgcgacg cccgagcata ttaatttccc aggtatgttt ctggcacatc gtttcatggt
     7621 tataaccata gaaattgagc gcggcgaggt tagcatcgac gatttggcca tctcgtgagg
     7681 ggtcaatcaa caacattggt gcagagttag tcagaaaaaa gcgcgcataa aaaccttgtt
     7741 ttttgcgctg ataatttgcc gagcgactgg cttttaaacc cagcgttgcc ggcgcttcga
     7801 taccttcgaa aataatcacc ggttctgttt ctgtcagctc tcgcaaaaca agccgacagc
     7861 ttaatgctgt ttcctcttct ttacgctgaa cagtgaggat ttcgataata tcgtgttggt
     7921 tttgcagatc ggagaggtat ttcggcagtt ctttttgtga ggagacggaa tagggtccgg
     7981 ttcgtagctg actaaacgtg aggtcttgca tcaacagtcg cgccgcgcta ttggcataaa
     8041 ttaactgttc ctcaaagggc gaaacaatcc agacaggact ggtgagtaag tccagggtat
     8101 tgaagttgtg cgtaatcatt gagatcccgt tatttttatc aatttttgtt gctatccgat
     8161 cgcaaaaaag ccacgtcata tgatcagata attctgataa tgatagacgc tattt
//
LOCUS       DAZKHF020000195         8133 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2201S31241
            SAMN43267800-rid25106413.denovo.196, whole genome shotgun sequence.
ACCESSION   DAZKHF020000195 DAZKHF020000000
VERSION     DAZKHF020000195.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267800
            Sequence Read Archive: SRR30307263
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 8133)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8133)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 8133)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350581.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350581.2
            Genome Coverage       :: 151x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:21:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,652
            CDSs (total)                      :: 5,557
            Genes (coding)                    :: 5,320
            CDSs (with protein)               :: 5,320
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 237
            CDSs (without protein)            :: 237
            Pseudo Genes (ambiguous residues) :: 0 of 237
            Pseudo Genes (frameshifted)       :: 102 of 237
            Pseudo Genes (incomplete)         :: 130 of 237
            Pseudo Genes (internal stop)      :: 52 of 237
            Pseudo Genes (multiple problems)  :: 41 of 237
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8133
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267800-rid25106413.denovo.196"
                     /serotype="O 26:H11"
                     /isolate="2201S31241"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-01"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            complement(103..981)
                     /gene="glxR"
                     /locus_tag="ACHOSC_RS17910"
                     /old_locus_tag="pgaptmp_003582"
     CDS             complement(103..981)
                     /gene="glxR"
                     /locus_tag="ACHOSC_RS17910"
                     /old_locus_tag="pgaptmp_003582"
                     /EC_number="1.1.1.60"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008679 - 2-hydroxy-3-oxopropionate
                     reductase activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0046487 - glyoxylate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxy-3-oxopropionate reductase"
                     /protein_id="HGU4549454.1"
                     /translation="MKLGFIGLGIMGTPMAINLARAGHQLHVTTIGPVADELLSLGAV
                     SVETARQVTEASDIIFIMVPDTPQVEEVLFGENGCTKASLKGKTIVDMSSISPIETKR
                     FARQVNELGGDYLDAPVSGGEIGAREGTLSIMVGGDEAVFERVKPLFELLGKNITLVG
                     GNGDGQTCKVANQIIVALNIEAVSEALLFASKAGADPVRVRQALMGGFASSRILEVHG
                     ERMIKRTFNPGFKIALHQKDLNLALQSAKALALNLPNTATCQELFNTCAANGGSQLDH
                     SALVQALELMANHKLA"
     gene            complement(1081..1857)
                     /gene="hyi"
                     /locus_tag="ACHOSC_RS17915"
                     /old_locus_tag="pgaptmp_003583"
     CDS             complement(1081..1857)
                     /gene="hyi"
                     /locus_tag="ACHOSC_RS17915"
                     /old_locus_tag="pgaptmp_003583"
                     /EC_number="5.3.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxypyruvate isomerase"
                     /protein_id="HGU4549455.1"
                     /translation="MLRFSANLSMLFGEYDFLARFEKAAQCGFRGVEFMFPYDYDIEE
                     LKQVLASNKLEHTLHNLPAGDWAAGERGIACIPGREEEFRDGVAAAIRYARALGNKKI
                     NCLVGKTPAGFSSEQIHATLVENLRYAANMLMKEDILLLIEPINHFDIPGFHLTGTRQ
                     ALKLIDDVGCCNLKIQYDIYHMQRMEGELTNTMTQWADKIGHLQIADNPHRGEPGTGE
                     INYDYLFKVIENSDYNGWVGCEYTPQTTTEAGLRWMDPYR"
     gene            complement(1870..3651)
                     /gene="gcl"
                     /locus_tag="ACHOSC_RS17920"
                     /old_locus_tag="pgaptmp_003584"
     CDS             complement(1870..3651)
                     /gene="gcl"
                     /locus_tag="ACHOSC_RS17920"
                     /old_locus_tag="pgaptmp_003584"
                     /EC_number="4.1.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009028 - tartronate-semialdehyde synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009436 - glyoxylate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glyoxylate carboligase"
                     /protein_id="HGU4549456.1"
                     /translation="MAKMRAVDAAMYVLEKEGITTAFGVPGAAINPFYSAMRKHGGIR
                     HILARHVEGASHMAEGYTRATAGNIGVCLGTSGPAGTDMITALYSASADSIPILCITG
                     QAPRARLHKEDFQAVDIEAIAKPVSKMAVTVREAALVPRVLQQAFHLMRSGRPGPVLV
                     DLPFDVQVAEIEFDPDMYEPLPVYKPAASRMQIEKAVEMLIQAERPVIVAGGGVINAD
                     AAALLQQFAELTSVPVIPTLMGWGCIPDDHELMAGMVGLQTAHRYGNATLLASDMVFG
                     IGNRFANRHTGSVEKYTEGRKIVHIDIEPTQIGRVLCPDLGIVSDAKAALTLLVEVAQ
                     EMQKAGRLPCRKEWVADCQQRKRTLLRKTHFDNVPVKPQRVYEEMNKAFGRDVCYVTT
                     IGLSQIAAAQMLHVFKDRHWINCGQAGPLGWTIPAALGVCAADPERNVVAISGDFDFQ
                     FLIEELAVGAQFNIPYIHVLVNNAYLGLIRQSQRAFDMDYCVQLAFENINSSEVNGYG
                     VDHVKVAEGLGCKAIRVFKPEDIAPAFEQAKALMAQYRVPVVVEVILERVTNISMGSE
                     LDNVMEFEDIADNAADAPTETCFMHYE"
     gene            complement(3741..4556)
                     /gene="allR"
                     /locus_tag="ACHOSC_RS17925"
                     /old_locus_tag="pgaptmp_003585"
     CDS             complement(3741..4556)
                     /gene="allR"
                     /locus_tag="ACHOSC_RS17925"
                     /old_locus_tag="pgaptmp_003585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional repressor AllR"
                     /protein_id="HGU4549457.1"
                     /translation="MTEVRRRGRPGQAEPVAQKGAQALERGIAILQYLEKSGGSSSVS
                     DISLNLDLPLSTTFRLLKVLQAADFVYQDSQLGWWHIGLGVFNVGAAYIHNRDVLSVA
                     GPFMRRLMLLSGETVNVAIRNGNEAVLIGQLECKSMVRMCAPLGSRLPLHASGAGKAL
                     LYPLAEEELMSIILQTGLQQFTPTTLVDMPTLLKDLEQARELGYTVDKEEHVVGLNCI
                     ASAIYDDVGSVVAAISISGPSSRLTEDRFVSQGELVRDTARDISTALGLKAHP"
     gene            complement(4634..5116)
                     /gene="allA"
                     /locus_tag="ACHOSC_RS17930"
                     /old_locus_tag="pgaptmp_003586"
     CDS             complement(4634..5116)
                     /gene="allA"
                     /locus_tag="ACHOSC_RS17930"
                     /old_locus_tag="pgaptmp_003586"
                     /EC_number="4.3.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ureidoglycolate lyase"
                     /protein_id="HGU4549458.1"
                     /translation="MKLQVLPLSQEAFSAYGDVIETQQRDFFHINNGLVERYHDLALV
                     EILEQDRTLISINRAQPANLPLTIHELERHPLGTQAFIPMKGEVFVVVVALGDDKPDL
                     STLRAFITNGEQGVNYHRNVWHHPLFAWQRVTDFLTIDRGGSDNCDVESIPEQELCFA
                     "
     gene            5346..6272
                     /gene="allS"
                     /locus_tag="ACHOSC_RS17935"
                     /old_locus_tag="pgaptmp_003587"
     CDS             5346..6272
                     /gene="allS"
                     /locus_tag="ACHOSC_RS17935"
                     /old_locus_tag="pgaptmp_003587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional activator AllS"
                     /protein_id="HGU4549459.1"
                     /translation="MFDPETLRTFIAVAETGSFSKAAERLCKTTATISYRIKLLEENT
                     GVALFFRTTRSVTLTAAGEHLLSQARDWLSWLESMPSELQQVNDGVERQVNIVINNLL
                     YNPQAVAQLLAWLNERYPFTQFHISRQIYMGVWDSLLYEGFSLAIGVTGTEALANTFS
                     LDPLGSVQWRFVMAADHPLANVEEPLTEAQLRRFPAVNIEDSARTLTKRVAWRLPGQK
                     EIIVPDMETKIAAHLAGVGIGFLPKSLCQSMIDNQQLVSRVIPTMRPPSPLSLAWRKF
                     GSGKAVEDIVTLFTQRRPEISGFLEIFGNPRS"
     gene            6341..7435
                     /gene="mnmH"
                     /locus_tag="ACHOSC_RS17940"
                     /old_locus_tag="pgaptmp_003588"
     CDS             6341..7435
                     /gene="mnmH"
                     /locus_tag="ACHOSC_RS17940"
                     /old_locus_tag="pgaptmp_003588"
                     /EC_number="2.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043828 - tRNA 2-selenouridine synthase
                     activity [Evidence IEA];
                     GO_process: GO:0002098 - tRNA wobble uridine modification
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-selenouridine(34) synthase MnmH"
                     /protein_id="HGU4549460.1"
                     /translation="MQERHTEQDYRALLIADTPIIDVRAPIEFEQGAMPAAINLPLMN
                     NDERAAVGTCYKQQGSDAALALGHKLVAGEIRQQRMDAWRAACLQNPQGILCCARGGQ
                     RSHIVQSWLYAAGIDYPLVEGGYKALRQTAIQATIELAQKPIVLIGGCTGSGKTLLVQ
                     QQPNGVDLEGLARHRGSAFGRTLQPQLSQASFENLLAAEMLKTDARQDLRLWVLEDES
                     RMIGSNHLPECLRERMTQAAIAVVEDPFEIRLERLNEEYFLRMHHDFTHAYGDEQGWQ
                     EYCEYLHHGLSAIKRRLGLQRYNELAAQLDTALTTQLTTGSTDGHLAWLVPLLKEYYD
                     PMYRYQLEKKAEKVVFRGEWAEVAEWVKAR"
     gene            7551..7916
                     /gene="ylbG"
                     /locus_tag="ACHOSC_RS17945"
                     /old_locus_tag="pgaptmp_003589"
                     /pseudo
     CDS             7551..7916
                     /gene="ylbG"
                     /locus_tag="ACHOSC_RS17945"
                     /old_locus_tag="pgaptmp_003589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014543172.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
     gene            7937..>8133
                     /locus_tag="ACHOSC_RS17950"
                     /old_locus_tag="pgaptmp_003590"
     CDS             7937..>8133
                     /locus_tag="ACHOSC_RS17950"
                     /old_locus_tag="pgaptmp_003590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001326183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4549461.1"
                     /translation="MTFRKKLYGVLDAVGATISTPAEPWTIGPPYWGMSPWRAAGFPL
                     MVYFLFLSRQEFILIRKDYLFS"
BASE COUNT         2054 a         2147 c         1957 g         1975 t
ORIGIN      
        1 aaaaatatta tttttaatac tgaagcaaca tctttggctg ctttcttttt taatattgct
       61 ggccgattca ttttctgata tcggccattt ttattgcggg tatcaggcca gtttatggtt
      121 agccattaat tccagcgcct gcactaacgc agagtgatcc aactggctgc caccgtttgc
      181 cgcgcaggta ttaaataact cctggcaggt cgcagtgttt ggcaggttca gcgctagcgc
      241 tttcgcactt tgcagtgcca ggttgagatc tttctggtgc agcgcaattt tgaaacctgg
      301 attaaaagtg cgtttaatca tgcgctcacc gtgaacttcc agaatacgtg aggaagcaaa
      361 gccgcccatc agcgcctggc gcacacgtac cgggtccgca ccggcttttg aagcaaatag
      421 cagggcttca gaaaccgctt caatattgag cgccacaata atctgatttg ccactttgca
      481 ggtttgacca tcgccgttac cgcccacgag ggtgatattt ttaccgagca gttcaaacag
      541 cggtttaaca cgttcaaata ccgcttcatc accgccaacc ataatcgaca acgtcccttc
      601 acgcgcaccg atttcaccgc cggagactgg cgcatcgaga taatcgccgc ccagttcatt
      661 cacctgacga gcgaaacgct tagtttcaat cggggaaatg gagctcatat caacaatggt
      721 tttgcccttc agcgaggctt tggtacaacc attttcaccg aacagaactt cttcaacctg
      781 tggtgtatct ggcaccataa taaaaatgat gtccgatgct tccgttacct ggcgcgcagt
      841 ttcaacactg acggcaccca gtgacagtaa ttcatcagca accggaccaa tggttgtgac
      901 atgcagttga tgaccggcac gcgccagatt aatggccatc ggtgtaccca taatgcctaa
      961 gccaataaat cccagtttca taattaacct cttttaaatt tcgcttttcc tgaattcaga
     1021 caacacgatc ccctgccaac ttagcggcct aaaaaagcat tgcctgaaca gcgttacgtt
     1081 ttaacggtac ggatccatcc agcgtaaacc ggcttccgtg gtggtttggg gtgtatattc
     1141 acacccaacc caaccgttgt agtcagaatt ttcgattacc ttaaagagat aatcataatt
     1201 aatttctccg gtccccggtt cgccgcgatg cggattatca gcaatttgca ggtgaccaat
     1261 tttatcggcc cactgagtca tggtgttggt taattcacct tccatccgct gcatgtgata
     1321 aatgtcatac tgaattttca aattgcagca accaacatca tcaatcaatt tcagcgcctg
     1381 ccgagttccg gtgagatgga aaccaggaat atcaaaatgg ttaataggtt caatcagtaa
     1441 taaaatatct tctttcatca gcatattcgc ggcataacgc aggttttcta caagcgttgc
     1501 gtgaatctgt tcactgctga aaccagccgg cgttttaccg accagacagt taattttttt
     1561 attacccagc gcacgggcat aacgaatcgc tgctgctacg ccatcccgaa actcttcttc
     1621 acggccagga atacaggcaa taccgcgctc ccccgccgcc cagtcacctg ccggtaaatt
     1681 gtgcagcgta tgttcgagtt tattactcgc cagcacctgt tttaattctt caatgtcgta
     1741 gtcataagga aacataaatt caacgccgcg aaaaccacac tgcgcagctt tctcaaaacg
     1801 ggcgagaaaa tcatattcac caaataacat cgataaatta gcagagaaac gtaacattat
     1861 ttatctccct tattcatagt gcatgaagca ggtttcagtc ggtgcgtccg ctgcgttatc
     1921 ggcgatatct tcaaattcca tgacgttatc cagttcgctg cccatcgaaa tattggtcac
     1981 acgctcaaga ataacttcca cgactaccgg tacccgatat tgcgccatta aggctttcgc
     2041 ctgttcaaag gctggcgcaa tatcttccgg tttgaagacc cgaatagctt tacaacctaa
     2101 accttccgct acttttacgt ggtcgacgcc gtaaccgttc acttcactgg agttaatatt
     2161 ttcgaacgcg agctgtacgc agtaatccat gtcaaaggcg cgttgtgact gacgaatcag
     2221 ccccagataa gcgttattga ccagtacatg gatgtacgga atgttgaact gtgcgccaac
     2281 ggctaattct tcaatcagga actggaagtc gaagtcaccg gaaatcgcca ccacattccg
     2341 ttctggatca gcagcacaca cgcccagcgc cgccggaatg gtccagccca gcgggcctgc
     2401 ctgaccacag ttgatccagt gacggtcttt aaagacatgc agcatttgtg ccgcagcgat
     2461 ttgtgacagg ccaatggtgg tgacataaca aacatcgcga ccaaaggctt tgttcatctc
     2521 ttcatacacg cgctgcggtt tcaccggcac gttgtcgaag tgggttttgc gcagcaaagt
     2581 gcgtttgcgc tgctggcagt cggcgaccca ttctttacga cacggcagac gacccgcttt
     2641 ttgcatctcc tgcgccactt caaccagcag tgtcagcgcc gctttagcat cagagacaat
     2701 gccgagatcc ggacacagca cgcgaccaat ttgcgtcggc tcaatatcga tatgaacgat
     2761 tttgcgccct tcggtgtatt tctctaccga accggtatga cggttagcaa aacggttacc
     2821 gataccaaac accatgtcgg acgccagcag cgttgcgtta ccgtaacgat gcgcggtttg
     2881 cagacccacc atcccggcca tcagttcatg atcgtccggg atacagcccc agcccatcag
     2941 cgttgggatc accggaacgc tggtcagttc agcaaactgt tgtaacagtg cagctgcgtc
     3001 agcattaatt accccgcccc cggcaacaat caccggacgt tcggcctgga ttaacatttc
     3061 tacagctttt tcgatctgca tacggctggc agcaggtttg tagaccggca gcggttcgta
     3121 catatcagga tcaaactcga tttccgcaac ctgaacgtcg aacggtaaat ccaccagtac
     3181 cggacccgga cgaccagaac gcatcaggtg aaatgcctgt tgcaacacac gaggcaccag
     3241 cgccgcttca cgtactgtaa ccgccatttt acttaccggc ttggcgatag cttcgatatc
     3301 aacagcctgg aaatcttctt tatgcagacg ggcgcgcgga gcctggccag taatacacag
     3361 aataggaatg gaatcagcag aagcagaata gagcgcggtg atcatgtccg tgcccgcagg
     3421 accggaagtc cccagacata cgccgatatt ccctgccgtt gcgcgggtat aaccttccgc
     3481 catgtgcgaa gcaccttcca catgacgcgc cagaatgtga cgaataccgc cgtgcttacg
     3541 catcgctgag tagaacggat tgattgcagc tcccggaacg ccgaaggcgg tggtgatacc
     3601 ttctttctcc agcacataca ttgccgcgtc aacggctctc atttttgcca ttttattcca
     3661 acctctatta attggaaaaa ttttccaact ttcaaatttt gtcaaaaaaa cccgctccgc
     3721 agagcgggat gcgacagaca ctatggatgt gctttcagtc ccaacgccgt gctgatatcg
     3781 cgggcggtgt ctctgaccag ctcaccctga ctgacaaaac gatcttctgt cagtcttgat
     3841 gaaggcccgg agatggagat agcggcaaca acgctaccga catcatcgta aattgctgaa
     3901 gctatgcaat tcagacctac aacatgctct tctttatcta cggtatagcc cagttcacgc
     3961 gcttgttcca ggtccttcag caaggtgggc atatccacaa gcgtagttgg cgtaaactgc
     4021 tgcaaaccgg tttgcagaat gatgctcatc aactcctctt ccgccagcgg ataaagcaac
     4081 gccttgcccg caccggaagc atgcagcggc agacgactgc ccagtggcgc acacatcctg
     4141 accatcgatt tacactctaa ctgaccaatt aataccgctt cattgccgtt acggatcgcg
     4201 acattgaccg tttcgccgga aagtaacatc aggcggcgca taaacggccc ggcgacggag
     4261 aggacatcgc ggttatggat gtacgccgca ccgacgttaa agacacctaa tcctatatgc
     4321 caccagccta actgactgtc ctgatagaca aaatccgctg cctgtaaaac cttcagcaag
     4381 cgaaaggtcg tagagagcgg caaatccaga ttgagagaaa tatcgctaac cgacgaactt
     4441 cccccacttt tttccagata ttgcagaatc gcaattcccc gctctaacgc ctgtgcgccc
     4501 ttctgtgcca caggctccgc ctgtcctggc ctgccgcgcc gtctaacttc cgtcatacct
     4561 acgttaactc cgacctacaa gtgagcataa acaatgaaca attagtttat cttatgcaag
     4621 tcggttgcag gcgtcacgca aaacagagtt cctgttcagg aatactttca acatcacagt
     4681 tgtcactgcc gccgcgatcg atggtcagaa aatcggtgac gcgctgccag gcgaaaagtg
     4741 ggtgatgcca gacgttacgg tggtaattca ctccctgttc gccgttggta ataaacgccc
     4801 gcagggttga caggtctggt ttgtcgtcac ctaacgccac gaccaccaca aacacctcac
     4861 ctttcatcgg gataaaggcc tgagtaccca gcggatgacg ttcgagttcg tgaatggtca
     4921 gcggcagatt cgccggttgc gcgcggttaa tgctgataag cgtacggtct tgctcaagaa
     4981 tctcaaccag cgccaaatcg tggtaacgct ccaccaggcc attgttaata tggaaaaaat
     5041 cccgttgctg cgtttcgatt acgtcgccat aagcactaaa ggcttcctga cttaacggta
     5101 atacctgaag tttcatcttt ttgtccttgt aacataattc cgttcaacca ccgccgaccg
     5161 gtcagcttgg tttcaataac aggcaatcta aaaccttaag agaacaacat caatacaaaa
     5221 gttggaatat ttttccaaaa ctagatttag tccacaaatt gaacagggtt atcatgtaaa
     5281 aatactgata aatcagagga taagaaaatc acgcaacttg cggatttaaa aggggatacc
     5341 tgaagatgtt cgatccagaa accttgcgga ctttcattgc ggttgctgaa acaggaagtt
     5401 tttcaaaagc ggcagaacga ttatgtaaaa ccacggcgac gatcagttat cgcattaaac
     5461 ttctggaaga gaataccgga gtagcgctgt ttttccgcac gactcgcagc gtgacgttga
     5521 cagcggctgg cgagcatcta ctttcccagg ccagagactg gctgagctgg ctggaaagta
     5581 tgccaagcga gctgcaacag gtgaatgatg gcgtggaacg ccaggtgaat attgtcatca
     5641 acaacctgct ctacaacccc caggccgtcg cccagttgct ggcgtggctg aatgaacgtt
     5701 acccctttac ccagtttcac atctcccgac aaatctatat gggcgtctgg gattcgctat
     5761 tatacgaagg tttttcactg gctatcggcg tcacgggaac cgaggcgctg gcaaatacct
     5821 ttagtcttga tcccttagga tcggtgcaat ggcgctttgt catggcggcg gatcatccgc
     5881 tggcgaacgt tgaagagccg ctaacagaag cgcagttgcg gcgctttccg gcggtcaata
     5941 ttgaagacag cgcccgcacc ttaaccaaac gcgtcgcctg gcgattgcct gggcaaaaag
     6001 agattattgt tcctgatatg gaaacgaaaa tcgccgccca tcttgcgggc gttggcattg
     6061 gttttttgcc aaaatcgctt tgccagtcaa tgatcgataa tcaacaacta gttagccggg
     6121 taatcccaac gatgcgccct ccttcgccat tgagtctggc atggcgcaaa tttggcagcg
     6181 gcaaagcggt agaagatatt gtgaccttgt ttacccagcg caggccggaa atcagcggat
     6241 ttttagaaat tttcggcaac ccacgcagtt aaaaatcaag tttccttgcg cgttggcgcg
     6301 taaaatagcg cgcaagctga tttgaggttc acttttaact atgcaagaga gacacacgga
     6361 acaggactat cgtgccctgc tgattgctga tacgcccatt attgatgttc gcgcccctat
     6421 cgagtttgag caaggcgcaa tgcccgccgc tatcaatctg ccgttaatga ataacgatga
     6481 acgcgccgcc gttggcacct gctataaaca gcaaggctca gacgcagcgc tggcgctggg
     6541 gcataaactg gtggcgggtg aaattcgtca gcagcgcatg gacgcctggc gggcagcgtg
     6601 cctgcaaaat ccgcaaggta ttctctgctg cgcccgtggc ggtcagcgct cacatattgt
     6661 gcaaagctgg ttgtatgcag cggggattga ttatccgctg gtggaaggcg gttataaggc
     6721 actgcgccag accgcgattc aggcgactat tgaactggca caaaaaccga tagtgctgat
     6781 tggcggttgt actggcagcg gtaaaacgct gttagtgcag caacagccga acggtgttga
     6841 tctggaaggg ctggcgcgcc atcgcggttc ggcgtttggt cgcacgttac aaccacaact
     6901 tagccaggcg agttttgaaa acctgctggc tgccgaaatg ctgaaaaccg acgcccgtca
     6961 ggatttgcgc ctgtgggtgc tggaagacga aagccggatg atcggttcga atcacctgcc
     7021 ggaatgcctg cgcgagcgaa tgactcaggc ggcgattgcg gtggtagaag atccgtttga
     7081 gatccgtctt gagcgcctga acgaagagta tttcttgcgt atgcatcatg attttaccca
     7141 cgcttatggc gacgaacagg gctggcagga atattgcgaa tacctgcatc acggactttc
     7201 ggcgattaag cgtcggctgg ggttacagcg ctataacgaa ctcgctgccc agctggatac
     7261 ggcgctgaca acgcaactca ccaccggcag caccgacggt catctggcct ggctggtgcc
     7321 gttacttaaa gaatattacg acccaatgta tcgctatcag cttgagaaaa aagcggaaaa
     7381 agttgtcttt cgcggtgagt gggcggaagt ggcggaatgg gttaaggcgc ggtaatgagg
     7441 gacgtatttg tattagtcgc gacttgatct gacacatggc cttgaaaggt tgagagttac
     7501 cggttttgat atgagtgtcg aatccttatc caaaacaaga ggtaactctc atgcttcata
     7561 ctgccaatcc cgtcatcaaa cacaaagccg gtttgctcaa tctggctgaa gaactcagca
     7621 acgtatcaaa agcctgtaaa atcatgggcg tttcgcgtga tacgttttat cgttaccgcg
     7681 aactggtcgc tgaaggcggt gtggatgcgc agattaatcg tagtcgccgc gctcctaacc
     7741 ttaagaaccg taccgatgag gcaactgaac aggctgttgt tgattacgcc gtcgctttcc
     7801 cgacacatgg tcagcaccgg gccagcaacg agctgcgtaa acagggggtt tttatctccg
     7861 acagtggtgt ccgttctgtc tggttactcc ataaccttga gaatctcaaa agacgatact
     7921 gaacaaattt tatcaggtga cgttccgtaa aaagttgtat ggagttctcg atgcagtcgg
     7981 cgcgactatc agtacccccg ctgaaccctg gactatcggg ccgccgtact ggggcatgtc
     8041 accctggcgt gccgctggtt ttccgctaat ggtttatttc ctctttcttt cccggcaaga
     8101 atttatttta atccggaagg attatttatt ttc
//
LOCUS       DAZKHF020000196         8062 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2201S31241
            SAMN43267800-rid25106413.denovo.198, whole genome shotgun sequence.
ACCESSION   DAZKHF020000196 DAZKHF020000000
VERSION     DAZKHF020000196.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267800
            Sequence Read Archive: SRR30307263
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 8062)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8062)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 8062)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350581.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350581.2
            Genome Coverage       :: 151x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:21:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,652
            CDSs (total)                      :: 5,557
            Genes (coding)                    :: 5,320
            CDSs (with protein)               :: 5,320
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 237
            CDSs (without protein)            :: 237
            Pseudo Genes (ambiguous residues) :: 0 of 237
            Pseudo Genes (frameshifted)       :: 102 of 237
            Pseudo Genes (incomplete)         :: 130 of 237
            Pseudo Genes (internal stop)      :: 52 of 237
            Pseudo Genes (multiple problems)  :: 41 of 237
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8062
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267800-rid25106413.denovo.198"
                     /serotype="O 26:H11"
                     /isolate="2201S31241"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-01"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            complement(251..514)
                     /gene="cedA"
                     /locus_tag="ACHOSC_RS17955"
                     /old_locus_tag="pgaptmp_003591"
     CDS             complement(251..514)
                     /gene="cedA"
                     /locus_tag="ACHOSC_RS17955"
                     /old_locus_tag="pgaptmp_003591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division activator CedA"
                     /protein_id="HGU4549462.1"
                     /translation="MRLVKPVMKKPLRQQNRQIISYVPRTEPAPPEHAIKMDSFRDVW
                     MLRGKYVAFVLMGESFLRSPAFTVPESAQRWANQIRQEGEVTE"
     gene            697..2958
                     /gene="katE"
                     /locus_tag="ACHOSC_RS17960"
                     /old_locus_tag="pgaptmp_003592"
     CDS             697..2958
                     /gene="katE"
                     /locus_tag="ACHOSC_RS17960"
                     /old_locus_tag="pgaptmp_003592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_025308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004096 - catalase activity [Evidence
                     IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="catalase HPII"
                     /protein_id="HGU4549463.1"
                     /translation="MSQHNEKNPHQHQSPLHDSSEAKPGMDSLAPEDGSHRPAAEPTP
                     PGAQPTAPGSLKAPDTRNEKLNSLEDVRKGSENYALTTNQGVRIADDQNSLRAGNRGP
                     TLLEDFILREKITHFDHERIPERIVHARGSAAHGYFQPYKSLSDITKADFLSDPNKIT
                     PVFVRFSTVQGGAGSADTVRDIRGFATKFYTEEGIFDLVGNNTPIFFIQDAHKFPDFV
                     HAVKPEPHWAIPQGQSAHDTFWDYVSLQPETLHNVMWAMSDRGIPRSYRTMEGFGIHT
                     FRLINAEGKATFVRFHWKPLAGKASLVWDEAQKLTGRDPDFHRRELWEAIEAGDFPEY
                     ELGFQLIPEEDEFKFDFDLLDPTKLIPEELVPVQRVGKMVLNRNPDNFFAENEQAAFH
                     PGHIVPGLDFTNDPLLQGRLFSYTDTQISRLGGPNFHEIPINRPTCPYHNFQRDGMHR
                     MGIDTNPANYEPNSINDNWPRETPPGPKRGGFESYQERVEGNKVRERSPSFGEYYSHP
                     RLFWLSQTPFEQRHIVDGFSFELSKVVRPYIRERVVDQLAHIDLTLAQAVAKNLGIEL
                     TDDQLNITPPPDVNGLKKDPSLSLYAIPDGDVKGRVVAILLNDEVRSADLLAILKALK
                     AKGVHAKLLYSRMGEVTADDGTVLPIAATFAGAPSLTVDAVIVPCGNIADIADNGDAN
                     YYLMEAYKHLKPIALAGDARKFKATIKVADQGEEGIVEADSADGSFMDELLTLMAAHR
                     VWSRIPKIDKIPA"
     gene            complement(3217..3975)
                     /gene="chbG"
                     /locus_tag="ACHOSC_RS17965"
                     /old_locus_tag="pgaptmp_003593"
     CDS             complement(3217..3975)
                     /gene="chbG"
                     /locus_tag="ACHOSC_RS17965"
                     /old_locus_tag="pgaptmp_003593"
                     /EC_number="3.5.1.105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016811 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds, in linear amides
                     [Evidence IEA];
                     GO_process: GO:0000272 - polysaccharide catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chitin disaccharide deacetylase"
                     /protein_id="HGU4549464.1"
                     /translation="MERLLIVNADDFGLSKGQNYGIIEACRNGIVTSTTALVNGQAID
                     HAVQLSRDEPSLAIGMHFVLTMGKPLTAMPGLTRDGVLGKWIWQLAEEDALPLEEITQ
                     ELASQYLRFIELFGRKPTHLDSHHHVHMFPQIFPIVARFAAEEGIALRIDRQPLSNAG
                     DLPANLRSSQGFSSAFYGEEISEALFLQVLDDASHRGDRSLEVMCHPAFIDNTIRQSA
                     YCFPRLTELDVLTSASLKGAIAQRGYRLGSYRDV"
     gene            complement(3988..5340)
                     /gene="celF"
                     /locus_tag="ACHOSC_RS17970"
                     /old_locus_tag="pgaptmp_003594"
                     /gene_synonym="chbF"
     CDS             complement(3988..5340)
                     /gene="celF"
                     /locus_tag="ACHOSC_RS17970"
                     /old_locus_tag="pgaptmp_003594"
                     /gene_synonym="chbF"
                     /EC_number="3.2.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase"
                     /protein_id="HGU4549465.1"
                     /translation="MSQKLKVVTIGGGSSYTPELLEGFIKRYHELPVSELWLVDVEDG
                     KEKLDIIFELCQRMIDNAGVPMKLYKTLDRREALKDADFVTTQLRVGQLPARELDERI
                     PLSHGYLGQETNGAGGLFKGLRTIPVIFDIVKDVEELCPNAWVINFTNPAGMVTEAVY
                     RHTGFKRFIGVCNIPIGMKMFIRDVLMLKDSDDLSIDLFGLNHMVFIKDVLVNGKSRF
                     AELLDGVASGQLKASSVKNIFDLPFSEGLIRSLNLLPCSYLLYYFKQKEMLAIEMGEY
                     YKGGARAQVVQKVEKQLFELYKNPELKVKPKELEQRGGAYYSDAACEVINAIYNDKQA
                     EHYVNIPHHGHIDNIPADWAVEMTCKLGRDGATPHPRITHFDDKVMGLIHTIKGFEIA
                     ASNAALSGEFNDVLLALNLSPLVHSDRDAELLAREMILAHEKWLPNFADCIAELKKAH
                     "
     gene            complement(5445..6287)
                     /gene="chbR"
                     /locus_tag="ACHOSC_RS17975"
                     /old_locus_tag="pgaptmp_003595"
     CDS             complement(5445..6287)
                     /gene="chbR"
                     /locus_tag="ACHOSC_RS17975"
                     /old_locus_tag="pgaptmp_003595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator ChbR"
                     /protein_id="HGU4549466.1"
                     /translation="MMQPVINAPEIATAREQQLFNGKNFHVFIYNKTESISGLHQHDY
                     YEFTLVLTGRYFQEINGKRVLLERGDFVFIPLGSHHQSFYEFGATRILNVGISKRFFE
                     QHYLPLLPYCFVASQVYRTNNAFLTYVETVISSLNFRETGLEEFVEMVTFYVINRLRH
                     YREEQVIDDVPQWLKSTVEKMHDKEQFSESALENMVTLSAKSQEYLTRATQRYYGKTP
                     MQIINEIRINFAKKQLEMTNYSVTDIAFEAGYSSPSLFIKTFKKLTSFTPKSYRKKLT
                     EFNQ"
     gene            complement(6295..6645)
                     /gene="chbA"
                     /locus_tag="ACHOSC_RS17980"
                     /old_locus_tag="pgaptmp_003596"
     CDS             complement(6295..6645)
                     /gene="chbA"
                     /locus_tag="ACHOSC_RS17980"
                     /old_locus_tag="pgaptmp_003596"
                     /EC_number="2.7.1.196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS N,N'-diacetylchitobiose transporter subunit
                     IIA"
                     /protein_id="HGU4549467.1"
                     /translation="MMDLDNIPDTQTEAEELEEVVMGLIINSGQARSLAYAALKQAKQ
                     GDFAAAKAMMDQSRMALNEAHLVQTKLIEGDAGEVKMKVSLVLVHAQDHLMTSMLARE
                     LITELIELHEKLKA"
     gene            complement(6696..8054)
                     /gene="chbC"
                     /locus_tag="ACHOSC_RS17985"
                     /old_locus_tag="pgaptmp_003597"
     CDS             complement(6696..8054)
                     /gene="chbC"
                     /locus_tag="ACHOSC_RS17985"
                     /old_locus_tag="pgaptmp_003597"
                     /EC_number="2.7.1.196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS N,N'-diacetylchitobiose transporter subunit
                     IIC"
                     /protein_id="HGU4549468.1"
                     /translation="MSNVIASLEKVLLPFAVKIGKQPHVNAIKNGFIRLMPLTLAGAM
                     FVLINNVFLSFGEGSFFYSLGIRLDASTIETLNGLKGIGGNVYNGTLGIMSLMAPFFI
                     GMALAEERKVDALAAGLLSVAAFMTVTPYSVGEAYAVGANWLGGANIISGIIIGLVVA
                     EMFTFIVRRNWVIKLPDSVPASVSRSFSALIPGFIILSVMGIIAWALNTWGTNFHQII
                     MDTISTPLASLGSVVGWAYVIFVPLLWFFGIHGALALTALDNGIMTPWALENIATYQQ
                     YGSVEAALAAGKTFHIWAKPMLDSFIFLGGSGATLGLILAIFIASRRADYRQVAKLAL
                     PSGIFQINEPILFGLPIIMNPVMFIPFVLVQPILAAITLAAYYMGIIPPVTNIAPWTM
                     PTGLGAFFNTNGSVAALLVALFNLGIATLIYLPFVVVANKAQNAIDKEESEEDIANAL
                     KF"
BASE COUNT         2179 a         2093 c         1798 g         1992 t
ORIGIN      
        1 taaatctaag tcatttacaa tataagttta agcgcgacgc cacaggatga actatcaaaa
       61 atagctcatc atgattagca aaacttaacc attttaaaat aaataaacaa ttaaagaaaa
      121 aagatcactt atttatagca atagatcgtc aaaggcagct ttttgttact ggtggtttga
      181 atgaatgtag caatgaaata cagaatttca ggtcatgtaa ctcccggcaa aaccgggagg
      241 tacgtaatct ttactcagtc acttcccctt cctggcggat ctgatttgcc caacgctggg
      301 cggattcagg cacagtaaac gccggtgagc gcagaaatga ctctcccatc agtacaaacg
      361 caacatattt gccacgcagc atccagacat cacgaaaaga atccatcttt atcgcatgtt
      421 ctggcggcgc gggttccgtg cgtgggacat agctaataat ctggcggttt tgctggcgga
      481 gcggtttctt cattactggc ttcactaaac gcatattaaa aatcagaaaa actgtagttt
      541 agccgattta gcccctgtac gtcccacttt gcgtgtattt cataacaccg tttccagaat
      601 agtctccgaa gcgggatctg gctgatggtc tatagttaga gggttttttg accaaaacag
      661 cggccctttc agtaataaat taaggagacg agttcaatgt cgcaacataa cgaaaagaac
      721 ccacatcagc accagtcacc actacacgat tccagcgaag cgaaaccggg gatggactca
      781 ctggcacctg aggacggatc tcatcgtcca gcggctgaac caacaccgcc aggtgcacaa
      841 cctaccgccc ctgggagcct gaaagcccct gatacacgta acgaaaaact taattctctg
      901 gaagacgtac gcaaaggcag tgaaaattat gcgctgacca ctaatcaggg cgtgcgcatc
      961 gccgatgatc aaaactcact gcgtgccggt aaccgtggtc caacgctgct ggaagatttt
     1021 attctgcgcg agaaaatcac ccactttgac catgagcgca tcccggaacg tattgttcat
     1081 gcacgcggat cagcagctca cggttatttc cagccatata aaagcttaag cgatatcacc
     1141 aaagcggatt tcctctcaga tccgaacaaa atcaccccag tatttgttag gttctctacc
     1201 gttcagggtg gcgctggctc tgccgatacc gtgcgtgata tccgtggttt tgccaccaag
     1261 ttctatactg aagagggtat ttttgacctc gttggcaata acacgccaat cttctttatc
     1321 caggatgcgc ataaattccc cgattttgtt catgcggtaa aaccagaacc gcactgggca
     1381 attccacaag ggcaaagtgc ccacgatact ttctgggatt atgtttctct gcaacctgaa
     1441 actctgcaca acgtgatgtg ggcgatgtcg gatcgcggca tcccccgcag ttaccgcacc
     1501 atggaaggct tcggtattca caccttccgc ctgattaatg ccgaagggaa ggcaacgttt
     1561 gtacgtttcc actggaaacc actggcaggt aaagcctcac tcgtttggga tgaagcacaa
     1621 aaactcaccg gacgtgaccc ggacttccac cgccgcgagt tgtgggaagc cattgaagca
     1681 ggcgattttc cggaatacga actgggcttc cagttgattc ctgaagaaga tgaattcaag
     1741 ttcgacttcg atcttctcga tccaaccaaa ctgatcccgg aagaactggt gcccgttcag
     1801 cgtgtcggca aaatggtgct caatcgcaat ccggataact tctttgccga aaacgaacaa
     1861 gcagcattcc atccaggtca tattgttcct ggtctggact tcaccaacga tccgctgttg
     1921 cagggacgtt tgttctccta taccgataca caaatcagtc gtcttggtgg gccgaatttc
     1981 catgagattc cgattaaccg tccgacctgc ccttaccaca acttccagcg tgatggcatg
     2041 catcgtatgg ggatcgacac taacccggcg aattacgaac cgaactcgat caacgataac
     2101 tggccgcgcg aaacaccgcc gggaccgaaa cgcggcggtt ttgaatcata tcaggaacgc
     2161 gtggaaggaa ataaagttcg tgagcgcagt ccatcgtttg gcgaatatta ttcccatccg
     2221 cgtctgttct ggctaagtca gacgccattc gagcagcgcc atattgtcga tggtttcagt
     2281 tttgagttaa gcaaagtcgt tcgtccgtat attcgtgagc gcgttgttga ccagctggcg
     2341 catattgatc tcactctggc ccaggcggtg gcgaaaaatc tcggtatcga actgactgac
     2401 gaccagctga atatcacccc acctccggac gtcaacggtc tgaaaaagga tccatcctta
     2461 agtttgtacg ccattcctga cggtgatgtg aaaggtcgcg tggtagcgat tttacttaat
     2521 gatgaagtga gatcggcaga ccttctggcc attctcaagg ccctgaaggc caaaggcgtt
     2581 catgccaaac tgctctactc ccgaatgggt gaagtgactg cggatgacgg tacggtgttg
     2641 cctatagccg ctacctttgc cggtgcacct tcgctgacgg tcgatgcggt cattgtccct
     2701 tgcggcaata tcgcggatat cgctgacaac ggcgatgcca actactacct gatggaagcc
     2761 tacaaacacc ttaaaccgat tgcgctggcg ggagacgcgc gcaagtttaa agcaacaatc
     2821 aaggtcgctg accagggtga agaagggatt gtggaagctg acagcgctga cggtagtttt
     2881 atggatgaac tgctaacgct gatggcagca caccgcgtgt ggtcacgcat tcctaagatt
     2941 gacaaaattc ctgcctgatg ggaggcggcg caattgcgcc gcctcaaatg atttacatag
     3001 tgcgctttgt ttatgccgga tgcggcgtga acgccttatc cggcctacaa agtcgtataa
     3061 attcaatgta ttgcagttta ttggtagata ctgataagcg aagcgcatca ggcagttttg
     3121 cgtttgtcag cagtctcaag cggcgcagtt acgccgcctt tgtaggaatt aatcgccgga
     3181 tgcaaggttc acgccgcatc tggcaaacat cctcacttac acatcccgat aactccccaa
     3241 ccgataacca cgctgagcga tagcaccttt caacgacgct gatgtcaaca catccagctc
     3301 cgttaagcgt gggaaacagt aagcactctg acggatagta ttatcgataa acgccggatg
     3361 acacatcacc tccagcgaac gatcaccccg atggcttgcg tcatccagca cttgcaggaa
     3421 cagtgcctcg ctaatctctt caccataaaa cgcgctactg aatccctgag aactgcgcag
     3481 attagccggt aaatcaccag cgttagatag cggctgacgg tcgatgcgca acgcaatccc
     3541 ctcttcagcc gcaaacctgg caacaatcgg gaaaatctgc gggaacatat gcacatgatg
     3601 atggctatca agatgcgtag gtttgcgtcc aaatagctca atgaaacgca aatactgact
     3661 ggcaagctcc tgagtaattt cttccagcgg taaagcatct tcttctgcca actgccagat
     3721 ccattttccc agcacaccat cgcgggttaa ccccggcata gctgtcaggg gcttgcccat
     3781 agtaaggaca aagtgcatcc ctatggccag actcggttca tcacgactca actgcaccgc
     3841 atggtcaata gcctgcccat tcaccagcgc cgtcgttgat gtgacaatcc cattgcgaca
     3901 ggcctcgata atgccgtagt tctgaccttt gcttaagcca aaatcatcgg cattaacaat
     3961 cagtaagcgt tccataatca gcctcggtta atgtgctttt ttaagctctg cgatgcagtc
     4021 ggcaaaattt ggcagccatt tctcgtgcgc cagaatcatc tcgcgtgcca gcagctcagc
     4081 atcgcgatcg gaatgcacca acggactaag gtttagcgcc agtaacacat cgttaaattc
     4141 tccgctaagt gcggcgttgc tggcagcaat ctcgaagcct ttaatggtgt gaatcagccc
     4201 catcacttta tcatcgaaat gcgtaatgcg cggatgtggc gtcgcgccat cgcgccccag
     4261 cttacaggtc atttctaccg cccagtctgc cggaatatta tcaatatgcc catgatgcgg
     4321 gatattaacg taatgttcag cttgcttgtc gttgtagata gcgttgatca cttcgcacgc
     4381 tgcatcagag taataagccc caccgcgctg ttccagttct ttcggcttaa ctttcaactc
     4441 cggattttta tacagctcaa aaagttgttt ctcgactttc tgtactacct gtgctcgtgc
     4501 gccgcctttg tagtattcgc ccatttcaat agccagcatc tctttctgct tgaagtaata
     4561 cagcagataa gaacatggca gcagatttaa cgaacgaatt aagccctcac taaatggcag
     4621 atcgaaaata ttttttacag aggacgcttt taactgccct gacgccacac catcaagcaa
     4681 ttcggcaaag cgcgacttgc catttaccag cacatcctta atgaacacca tatggttgag
     4741 gccgaacaga tcgatagata aatcatcgct gtctttcagc atcagaacat cgcgaataaa
     4801 catcttcatg ccgatcggaa tattacacac gccgataaag cgtttaaatc cggtatgacg
     4861 ataaacggct tcagtgacca ttcccgccgg gttagtgaag ttaatcaccc atgcattcgg
     4921 acaaagttct tcgacatctt ttacgatgtc aaaaatcacc ggaatggtac gcagaccttt
     4981 aaacagaccg cccgcgccgt tggtttcctg accaagataa ccatgactta atggaatacg
     5041 ttcatccagt tcgcgcgccg gtaattggcc tacgcgcagt tgggtagtaa cgaaatcagc
     5101 atctttcaat gcttcgcggc gatccagcgt tttataaagc ttcatcggga cgccagcgtt
     5161 atcaatcatc cgttggcaga gttcaaaaat gatatccagt ttctctttac catcttcgac
     5221 atccaccagc cataattcgc tgaccggcaa ttcgtgataa cgcttaataa atccttccag
     5281 taactccggg gtatagctgc tcccgccacc aatagtgacg acttttaatt tctggctcat
     5341 aatttctccc ttcagtacag aatactgata tctggcatat ctgccccccc ggacataaat
     5401 aatccagcaa caggacagat atgtgaattg tcaggtataa cgacttactg attaaattca
     5461 gtcaatttct tacgatagct cttaggcgta aaggacgtta atttcttaaa cgttttaata
     5521 aacaaactcg ggctactata accggcctca aacgcaatat ccgtcacgga atagttggtc
     5581 atttccagtt gttttttggc aaaattaata cggatctcat taataatctg cattggtgtt
     5641 ttgccataat atcgttgagt ggctcgcgtc aaatattcct gtgatttggc tgacaacgtc
     5701 accatattct ccagcgccga ttcactaaac tgctctttat catgcatctt ttctaccgta
     5761 cttttcagcc actgcggtac atcatcaatc acctgttctt cgcggtaatg acgtaaacgg
     5821 ttaatgacat aaaaagtaac catctcaaca aactcttcca gccctgtttc gcggaaattc
     5881 aatgaagaaa tcactgtttc cacataggtg agaaaagcgt tattggtccg gtatacctgc
     5941 gaagcgacaa agcaataagg caacaacggc aggtaatgct gctcaaaaaa gcgtttactg
     6001 atcccaacgt tcaatatacg cgtggcacca aactcataaa aactttgatg gtgcgaacct
     6061 aacggaataa aaacaaaatc gccccgttcc agtaacacgc gcttaccgtt aatctcctgg
     6121 aaataacgcc cggttaatac cagagtaaat tcataatagt cgtgctgatg cagcccgctg
     6181 atactctcag ttttgttata gataaacaca tggaagtttt tgccattaaa caactgctgt
     6241 tctcgggcag tggcaatttc cggcgcgtta atcactggct gcatcatcga ctccttatgc
     6301 cttcagtttt tcatgaagct caattaattc agtaatcagt tcacgcgcaa gcatggacgt
     6361 cattaaatga tcctgagcgt ggaccagcac cagactcact ttcatcttaa cttcgcccgc
     6421 atcgccttca atcagtttcg tctgtaccag atgcgcttca ttcaatgcca tccgtgactg
     6481 atccatcatg gcttttgctg cagcaaaatc gccctgcttc gcctgtttca gtgccgcata
     6541 cgccaggctg cgcgcttgtc cggagttgat gataagcccc atcaccactt cttccagctc
     6601 ttcagcttct gtttgcgtat caggaatatt atcgagatcc atcatacatc gttcctctct
     6661 cttcttaccg gcaagattac ccgtaccggc atcgattaaa atttcagggc gttagcgata
     6721 tcttcttcgc tctcttcttt atcaatcgca ttttgtgctt tgttagccac cacaacaaag
     6781 ggcagataaa ttaaggttgc gatgccaagg ttgaagagtg cgaccagcaa tgcggcgacg
     6841 ctaccgttgg tgttaaagaa ggctcccaga ccggttggca tggtccacgg tgcaatattg
     6901 gtcaccggag gaatgatgcc catgtagtac gctgcgaggg tgattgccgc cagaatcggt
     6961 tgtaccagta caaacgggat aaacatcacc gggttcatga taattggcag accaaacaga
     7021 atcggttcgt taatctggaa gatgccggac ggcagcgcca gttttgccac ctgacgataa
     7081 tcagcacggc gagaggcgat aaagatagcc aggatcaggc ctaaagtcgc accactgccc
     7141 ccaaggaaaa taaaggagtc cagcatcggc ttggcccaga tatggaaggt cttaccggct
     7201 gccagcgccg cttcgacgga accatattgc tgataggtcg cgatattttc cagcgcccac
     7261 ggcgtcataa tgccgttgtc cagtgcggtc agcgccagcg cgccatgaat accgaagaac
     7321 cagagcagtg gaacaaagat cacataggcc cagcccacca cgctacccaa cgatgccagt
     7381 ggggttgaga tggtatccat aatgatctga tggaagttgg tgccccaggt attcaacgcc
     7441 caggcaataa tccccatcac ggaaagaata ataaagccgg gaattaatgc cgagaaggaa
     7501 cgcgatactg aagcaggtac gctgtcgggc agtttaatga cccaattgcg gcggacaata
     7561 aaggtaaaca tttctgccac caccaggcca ataataatcc cggagatgat attcgcccca
     7621 cctaaccagt ttgcgccaac cgcataggcc tcaccgacac tatatggggt gacggtcata
     7681 aatgctgcaa cggataacaa ccccgccgcc agcgcatcga ctttacgctc ttctgccagc
     7741 gccatgccaa taaagaacgg tgccattaaa gacattatcc ctaatgttcc gttatatacg
     7801 ttgccgccaa tacctttcag gccattaagt gtttcaatgg ttgaggcgtc gaggcgaata
     7861 cctaaggaat aaaaaaacga cccctcccca aagcttagaa aaacgttgtt aattaataca
     7921 aacatggccc ccgcaagggt taacggcatt aagcgaataa agccattttt gattgcatta
     7981 acgtgtggct gctttcctat tttaactgca aaagggagga gtaccttttc aagcgatgca
     8041 ataacattac tcatagaaaa at
//
LOCUS       DAZKHF020000197         8039 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2201S31241
            SAMN43267800-rid25106413.denovo.199, whole genome shotgun sequence.
ACCESSION   DAZKHF020000197 DAZKHF020000000
VERSION     DAZKHF020000197.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267800
            Sequence Read Archive: SRR30307263
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 8039)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8039)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 8039)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350581.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350581.2
            Genome Coverage       :: 151x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:21:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,652
            CDSs (total)                      :: 5,557
            Genes (coding)                    :: 5,320
            CDSs (with protein)               :: 5,320
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 237
            CDSs (without protein)            :: 237
            Pseudo Genes (ambiguous residues) :: 0 of 237
            Pseudo Genes (frameshifted)       :: 102 of 237
            Pseudo Genes (incomplete)         :: 130 of 237
            Pseudo Genes (internal stop)      :: 52 of 237
            Pseudo Genes (multiple problems)  :: 41 of 237
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8039
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267800-rid25106413.denovo.199"
                     /serotype="O 26:H11"
                     /isolate="2201S31241"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-01"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            336..1364
                     /gene="torT"
                     /locus_tag="ACHOSC_RS17990"
                     /old_locus_tag="pgaptmp_003598"
     CDS             336..1364
                     /gene="torT"
                     /locus_tag="ACHOSC_RS17990"
                     /old_locus_tag="pgaptmp_003598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TMAO reductase system periplasmic protein TorT"
                     /protein_id="HGU4549469.1"
                     /translation="MRVLLFLLLSLFMLSAFSADNLLRWHDAQHYTVQASTPLKAKRA
                     WKLCALYPSLKDSYWLSLNYGMQEAARRYGVDLKVLEAGGYSQLATQQAQIDQCKQWG
                     AEAILLGSSTTSFPDLQKQVANLPVIELVNALDDPLVKSRVGVPWFQMGYQPGRYLVQ
                     WAHGKPLNVLLMPGPDNAGGSKEMVEGFRAAIAGSPVRIVDIALGDNDIEIQRNLLQE
                     MLERHPEIDVVAGTAIAAEAAMGEGRNLKTPLTVVSFYLSHQVYRGLKRGRVIMAASD
                     QMVWQGELAVEQAIRQLQGQSVSDNVSPPILVLTPKNADREHIRRSLSPGGFRPVYFY
                     QHTSAAKK"
     gene            complement(1337..2029)
                     /gene="torR"
                     /locus_tag="ACHOSC_RS17995"
                     /old_locus_tag="pgaptmp_003599"
     CDS             complement(1337..2029)
                     /gene="torR"
                     /locus_tag="ACHOSC_RS17995"
                     /old_locus_tag="pgaptmp_003599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator TorR"
                     /protein_id="HGU4549470.1"
                     /translation="MPHHIVIVEDEPVTQARLQSYFTQEGYTVSVTASGAGLREIMQN
                     QPVDLILLDINLPDENGLMLTRALRERSTVGIILVTGRSDRIDRIVGLEMGADDYVTK
                     PLELRELVVRVKNLLWRIDLARQAQPHTQDNCYRFAGYCLNVSRHTLERDGEPIKLTR
                     AEYEMLVAFVTNPGEILSRERLLRMLSARRVENPDLRTVDVLIRRLRHKLSADLLVTQ
                     HGEGYFLAADVC"
     gene            2189..3361
                     /gene="torC"
                     /locus_tag="ACHOSC_RS18000"
                     /old_locus_tag="pgaptmp_003600"
     CDS             2189..3361
                     /gene="torC"
                     /locus_tag="ACHOSC_RS18000"
                     /old_locus_tag="pgaptmp_003600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pentaheme c-type cytochrome TorC"
                     /protein_id="HGU4549471.1"
                     /translation="MRKLWNALRRPSARWSVLALVAIGIVIGIALIVLPHVGIKVTST
                     TEFCVSCHSMQPVYEEYKQSVHFQNASGVRAECHDCHIPPDIPGMVKRKLEASNDIYQ
                     TFIAHSIDTPEKFEAKRAELAEREWARMKENNSATCRSCHNYDAMDHAKQHPEAARQM
                     KVAAKDNQSCIDCHKGIAHQLPDMSSGFRKQFDELRASANDSGDTLYSIDIKPIYAAK
                     GDKEASGSLLPASEVKVLKRDGDWLQIEITGWTESAGRQRVLTQFPGKRIFVASIRGD
                     VQQQVKTLEKTTVADTNTEWSKLQATAWMKKGDMVNDIKPIWAYADSLYNGTCNQCHG
                     APEIAHFDANGWIGTLNGMIGFTSLDKREERTLLKYLQMNASDTAGKAHGDKKEEK"
     gene            3361..5915
                     /gene="torA"
                     /locus_tag="ACHOSC_RS18005"
                     /old_locus_tag="pgaptmp_003601"
                     /pseudo
     CDS             3361..5915
                     /gene="torA"
                     /locus_tag="ACHOSC_RS18005"
                     /old_locus_tag="pgaptmp_003601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415517.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA];
                     GO_function: GO:0030151 - molybdenum ion binding [Evidence
                     IEA];
                     GO_function: GO:0043546 - molybdopterin cofactor binding
                     [Evidence IEA];
                     GO_function: GO:0050626 - trimethylamine-N-oxide reductase
                     (cytochrome c) activity [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="trimethylamine-N-oxide reductase TorA"
     gene            5912..6511
                     /gene="torD"
                     /locus_tag="ACHOSC_RS18010"
                     /old_locus_tag="pgaptmp_003602"
     CDS             5912..6511
                     /gene="torD"
                     /locus_tag="ACHOSC_RS18010"
                     /old_locus_tag="pgaptmp_003602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0051259 - protein complex oligomerization
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone TorD"
                     /protein_id="HGU4549472.1"
                     /translation="MTTLTAQQIACVYAWLAQLFSRELDDEQLTQIASAQMAEWFSLL
                     KSEPPLAAAVNELENCIATLTVRDDARLELAADFCGLFLMTDKQAALPYASAYKQDEQ
                     EIKRLLVEAGMETSGNFNEPADHLAIYLELLSHLHFSLGEGTVPARRIDSLRQKTLTA
                     LWQWLPEFVVRCRQYDSFGFYAALSQLLLVLVESDHQNR"
     gene            complement(6604..6909)
                     /gene="cbpM"
                     /locus_tag="ACHOSC_RS18015"
                     /old_locus_tag="pgaptmp_003603"
     CDS             complement(6604..6909)
                     /gene="cbpM"
                     /locus_tag="ACHOSC_RS18015"
                     /old_locus_tag="pgaptmp_003603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chaperone modulator CbpM"
                     /protein_id="HGU4549473.1"
                     /translation="MANVTVTFTITEFCLHTGISEEELNEIVGLGVVEPREIQETTWV
                     FDDHAAIVVQRAVRLRHELALDWPGIAVALTLMDDIAHLKQENRLLRQRLSRFVTHP"
     gene            complement(6909..7829)
                     /gene="cbpA"
                     /locus_tag="ACHOSC_RS18020"
                     /old_locus_tag="pgaptmp_003604"
     CDS             complement(6909..7829)
                     /gene="cbpA"
                     /locus_tag="ACHOSC_RS18020"
                     /old_locus_tag="pgaptmp_003604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="curved DNA-binding protein"
                     /protein_id="HGU4549474.1"
                     /translation="MELKDYYAIMGVKPTDDLKTIKTAYRRLARKYHPDVSKEPDAEA
                     RFKEVAEAWEVLSDEQRRAEYDQMWQHRNDPQFNRQFHHSDGQSFNAEDFDDIFSSIF
                     GQHARQSRQRPATRGHDIEIEVAVFLEETLTEHKRTISYNLPVYNAFGMIEQEIPKTL
                     NVKIPAGVGNGQRIRLKGQGTPGENGGPNGDLWLVIHIAPHPLFDIVGHDLEIVVPVS
                     PWEAALGAKVTVPTLKESILLTIPPGSQAGQRLRVKGKGLVSKKQTGDLYAVLKIVMP
                     PKPDENTAALWQQLADAQSSFDPRKDWGKA"
BASE COUNT         1937 a         2004 c         2194 g         1904 t
ORIGIN      
        1 aggcatagcc ataatgcatg atggtgtggc gtggcaaagc agagatatac aaagtctgcc
       61 acatgttttt atgacaaaaa attagcgcaa gaagacaaaa aatcaccttg cgctaatgct
      121 ctgttgtagg tcactaacca catctaactc attgattaat agtgactcta gatgtagtgt
      181 tttatcgtat tttgcagtct gttatttaat catgaattca tgtaacaata tgatttttaa
      241 aatatttgtg tgtatttgtc atcctttagg tgaaaaaggt tgagtcgcaa agcggaatgc
      301 atctagcata aagccttatt attgatgagg ctatcatgcg cgtactgcta tttttacttc
      361 tttccctttt catgttatcg gcattttcgg ctgataacct gttgcgctgg catgatgcgc
      421 agcattacac ggtgcaagcc tctacgccgc ttaaagccaa acgcgcatgg aaactgtgcg
      481 cgctttatcc cagcctgaaa gattcatact ggttatcgtt gaactatggt atgcaggagg
      541 cagctcgccg ctatggtgtg gatttaaaag tgctggaggc aggcggctac agccagttgg
      601 ctacccagca agcacaaatc gaccagtgta aacagtgggg cgcagaggcc attttgctcg
      661 gtagtagcac tacgtcattt cccgacctgc aaaagcaggt agcaaatctg ccggtgatcg
      721 aactggtaaa tgctcttgat gatcccctgg tgaaaagccg cgttggtgta ccctggtttc
      781 agatgggcta tcaacctggg cgatatttgg tgcaatgggc gcacggtaaa ccactgaacg
      841 tgctgttgat gcccggaccc gataatgccg ggggcagtaa agagatggtc gagggttttc
      901 gcgcagccat tgccggaagc ccggtgcgta ttgttgatat tgcgcttggt gataacgata
      961 ttgaaatcca gcgtaacctg ttgcaggaga tgctggaacg ccacccagaa atcgacgtcg
     1021 ttgccggaac ggccattgcg gcagaggcgg caatgggtga agggcgtaac ctgaaaacgc
     1081 cgcttaccgt ggtgtcgttt tatctttcac atcaggtgta tcgcgggctg aagcggggaa
     1141 gagtgattat ggctgccagc gatcaaatgg tctggcaggg ggaactggcg gttgagcagg
     1201 ccatcaggca attacagggg caatcggtgt ctgataatgt cagcccaccg attttagttc
     1261 tgacgccgaa aaatgccgac cgtgaacata ttcgccgctc gctgtcacca gggggatttc
     1321 gtccggtcta tttttatcag cacacatcag cggctaagaa ataaccttca ccatgttgcg
     1381 tcaccagtaa atccgcgctg agtttatgac gtaaacgacg aattaacaca tcgacggtgc
     1441 gcaggtcagg gttttccacc cgacgcgcag aaagcatacg tagcagacgt tcacggctga
     1501 gaatttcgcc cggattcgtc acaaatgcca ccaacatttc atactctgcg cgggtcagtt
     1561 taatcggctc gccatcccgc tccagcgtat ggcgcgacac attcaggcaa taaccggcaa
     1621 agcgatagca gttgtcctga gtgtgcggtt gagcttgtcg cgcgaggtcg attcgccaga
     1681 gcagattttt cacccgtact accagttcgc gcagttccag cggtttggtg acgtaatcgt
     1741 ctgcgcccat ttccagccca acaatacggt caatccgatc gctgcgtccg gtaaccagaa
     1801 taatccccac cgttgagcgt tctcgcaggg cgcgggttaa catcaggcca ttttcatcgg
     1861 gtaagttgat atccagcaga attaaatcta ccggctgatt ctgcataatt tcccgcagcc
     1921 cggcaccgct cgctgtaacg gaaacggtat acccctcctg agtgaagtaa gattgtaatc
     1981 gcgcctgggt aaccggctca tcttcaacaa taacaatgtg atgtggcatc agagggtttt
     2041 actcattctg ttcatatctg ttcatatctg ttcatatctg ttcatatctg ttcatattct
     2101 gccgtaagcc gttcatcctg accagtgccg ctgttcatat ttgctcatta agatcgcttc
     2161 actaaaccat aattttacag gggctattat gcggaaactc tggaacgcgc tacgccgacc
     2221 cagtgctcgt tggtcggtac tggcgctggt cgcaattggg attgtgattg gcattgcgct
     2281 gattgtattg ccacacgttg ggatcaaagt caccagcaca accgaatttt gtgtcagttg
     2341 ccacagtatg caaccggtgt atgaagaata taaacagtcg gtgcatttcc agaacgcctc
     2401 cggcgtgcga gctgaatgcc atgactgtca tatcccgccg gatattccag gcatggtgaa
     2461 gcgcaaactg gaagcgagca atgatatcta ccagaccttt attgctcact ccattgatac
     2521 acctgaaaaa ttcgaagcca aacgcgcgga acttgccgag cgtgaatggg cgcgaatgaa
     2581 agaaaacaac tcggcaacct gccgctcctg ccataactac gatgcgatgg atcatgcgaa
     2641 gcagcatcct gaagcggcac gtcagatgaa ggtggcagcg aaagataatc aatcctgcat
     2701 cgactgtcat aaaggtattg cccaccagtt accggatatg agtagcggct tccgtaagca
     2761 gttcgatgag ctgcgcgcca gtgctaatga cagtggtgac acgctgtact ctattgatat
     2821 taagccaatt tatgcggcga aaggcgataa agaagcctct ggttctctgc tgcctgcttc
     2881 ggaagtgaaa gtccttaaac gtgacggcga ctggctgcaa attgaaatta ccggctggac
     2941 ggaaagcgcc ggacgtcagc gtgtactcac ccaattccca ggtaaacgca tctttgttgc
     3001 ctcgattcgt ggtgatgtgc agcagcaggt aaaaacgctg gagaaaacca ccgttgccga
     3061 caccaatacc gagtggagca agttgcaggc cactgcgtgg atgaagaaag gcgacatggt
     3121 gaacgatatc aaaccgatct gggcttatgc ggattcgttg tacaacggca cctgtaacca
     3181 gtgccacggt gcaccggaaa tcgcccactt tgacgctaac ggttggatcg gcacgctcaa
     3241 cggcatgatt ggctttacca gtctcgataa acgtgaagaa cgcaccttgt tgaaatatct
     3301 gcaaatgaat gcgtctgaca ccgcaggtaa ggctcacggc gataagaagg aagaaaaata
     3361 atgaacaata acgatctctt tcaggcatca cgtcggcgtt ttctggcaca actcggcggc
     3421 ttaaccgtcg ccgggatgct ggggccgtca ttgttaacgc cgcgccgtgc gactgcggcg
     3481 caagcggcga ctgacgctgt catctcgaaa gagggcattc ttaccgggtc gcactggggg
     3541 gctatccgcg cgacggtgaa ggatggtcgc tttgtggcgg caaaaccgtt cgaactggat
     3601 aaatatccgt cgaaaatgat tgccggattg ccggattgcc ggatcacgta cacaacgcgg
     3661 cgcgtattcg ttatccgatg gtacgcgtgg actggctgcg taagcgccat ctcagcgata
     3721 cctcccagcg cggtgataac cgttttgtgc gcgtgagctg ggatgaagcc ctcgacatgt
     3781 tctatgaaga actggaacgc gtgcagaaaa ctcacgggcc gagtgccttg ctgaccgcca
     3841 gtggttggca atcgacgggg atgttccata acgcttcggg gatgctggcg aaagctattg
     3901 ccttgcatgg taatagcgtt ggtacgggcg gagattactc taccggtgct gcgcaggtga
     3961 tcctgccgcg cgtggttggc tcaatggaag tgtatgaaca gcaaacctcc tggccgctgg
     4021 tattgcagaa cagcaaaacc attgtgctgt ggggctctga tttgctgaaa aaccagcaag
     4081 cgaactggtg gtgcccggat cacgatgttt atgaatatta cgcgcagcta aaagcgaaag
     4141 tcgccgccgg tgaaattgag gtcatcagca tcgatccggt tgtcacatcc acccatgagt
     4201 atctggggcg cgagcatgtg aagcacattg cggttaaccc gcaaactgat gtgccgctgc
     4261 aactggcgct ggcgtatacg ctgtacagtg aaaacctgta cgacaaaaac ttcctcgcta
     4321 actactgtgt gggttttgag cagttcctgc cgtacctgct gggtgagaaa gacggtcagc
     4381 cgaaagatgc cgcatgggct gaaaaactga ccggcattga tgccgaaacc attcgtgggc
     4441 tggcgcggca gatggcggcg aacagaacgc aaattattgc tggctggtgc gtgcagcgta
     4501 tgcagcacgg tgaacagtgg gcgtggatga ttgtggttct ggcggcgatg ctggggcaaa
     4561 ttggcctgcc aggtggtggc tttggttttg gctggcacta taacggcgca ggcacgccgg
     4621 ggcgtaaagg cgttattctg agtggtttct ccggctctac gtcgattccg cctgttcacg
     4681 acaacagtga ttacaaaggc tacagcagca ccattccgat tgcccgtttt atcgatgcga
     4741 tcctcgaacc ggggaaagtg atcaactgga acggtaaatc ggtaaaactg ccgccgctga
     4801 aaatgtgtat ttttgccgga actaacccat tccatcgcca tcagcagatc aaccgcatta
     4861 ttgaaggctg gcgcaagctg gaaacggtta tcgccataga taaccagtgg acctcaacct
     4921 gccgctttgc cgatatcgtg ctgcctgcga ccacgcagtt tgagcgtaac gatctcgacc
     4981 agtacggcaa ccactccaac cgtggcatta tcgccatgaa acaggtggtg ccgccgcagt
     5041 tcgaggcgcg caacgacttt gatattttcc gcgagctgtg ccgtcgcttt aatcgcgaag
     5101 aagcctttac cgaagggctg gacgaaatgg gctggctgaa acgcatctgg caggaaggtg
     5161 tacaacaagg caaaggacgc ggcgttcatc tgccagcgtt tgatgacttc tggaataaca
     5221 aagagtacgt cgagtttgac catccgcaga tgtttgttcg ccaccaggca ttccgcgaag
     5281 atccggatct cgaaccgctg ggcacgccga gtggcctgat tgagatctac tcgaaaacca
     5341 tcgccgatat gaactacgac gattgtcagg ggcatccgat gtggtttgag aaaatcgaac
     5401 gctcccacgg tgggcccggc tcgcagaagt atccgttgca tctgcaatct gtgcatccgg
     5461 atttccgact tcactcgcag ttatgtgagt cggaaactct gcgtcagcaa tatacggtag
     5521 cgggtaaaga gccagtgttc attaacccgc aggatgccag cgcgcgcggt attcgtaacg
     5581 gtgatgtggt acgcgtcttt aacgctcgcg gtcaggtgtt ggcaggggca gtagtttctg
     5641 accgctatgc acccggcgtg gcacgaattc acgaaggggc atggtacgat ccagataaag
     5701 gcggcgagcc tggtgcgctg tgcaaatacg gtaaccccaa cgtgctgacc atcgacatcg
     5761 gtacttcgca gctggcgcag gcgaccagtg cgcacactac gctggtggaa attgagaagt
     5821 acaatggagc agtggagcag gtgacggcgt ttaacggccc tgtggagatg gtggcgcagt
     5881 gcgaatatgt tcccgcgtcg caggtgaaat catgaccacg ctgacagcac aacagattgc
     5941 ctgtgtttac gcctggctgg cgcagttgtt ctcccgtgaa ctggacgatg aacaactgac
     6001 gcaaatcgcc agtgcgcaga tggctgaatg gttttcgttg ctgaaaagcg aaccgccgct
     6061 ggctgcggcg gtgaatgagc tggaaaactg tattgccacg ctgacagtac gtgacgatgc
     6121 ccgtctggaa ctggccgctg acttttgcgg cctgtttctg atgaccgaca aacaagcggc
     6181 gctgccgtat gcatcggcct acaaacagga cgagcaagag attaaacgct tgttagttga
     6241 ggcagggatg gaaaccagcg gcaatttcaa cgaaccggca gatcatctgg cgatctatct
     6301 cgaattgctc agccatctgc atttttcgct gggagagggg accgttcctg cgcgaagaat
     6361 cgacagtttg cggcaaaaaa cactgacggc gctgtggcaa tggttaccag agtttgttgt
     6421 gcgttgtcgt cagtatgaca gctttggttt ttacgcggca ctaagccagt tattgctggt
     6481 gttagtggag agcgaccacc aaaacagata acgtcgtttg tgcgcctgaa aagacgcgtt
     6541 tagcgtcgca tcaggcatta tggggcagtt gccggatgcg tcatcacatc cggcaaacac
     6601 acatcacgga tgagttacaa accgggaaag tcgctggcgc agcaggcggt tttcctgctt
     6661 caggtgcgca atatcatcca ttaacgtcag cgccaccgcg atcccaggcc agtccagagc
     6721 cagttcatga cgcaggcgta ctgcgcgttg caccacgatg gcggcatggt cgtcaaatac
     6781 ccaggttgtt tcctggatct cacgcggttc aaccaccccc aaaccgacaa tttcattcaa
     6841 ctcctcttca gagatgccgg tatgcaggca aaattcggta atagtaaaag tcaccgtaac
     6901 attagccatt atgctttccc ccaatcttta cgtggatcaa aagacgactg ggcgtctgcc
     6961 agttgctgcc acagcgcggc agtgttttca tccggtttcg gcggcatcac gattttcagt
     7021 accgcataca gatcgccggt ctgttttttg ctcaccagac ctttgccttt aacgcgcaat
     7081 cgttgcccgg cctggctgcc tggcgggata gtcagcaaaa tgctttcttt cagtgttgga
     7141 acggtgactt tagcacccag cgccgcttcc cacgggctaa ccggcactac aatttccaga
     7201 tcatggccga caatatcgaa cagcggatgt ggcgcaatat gaatcaccag ccacaaatcg
     7261 ccatttggac cgccgttttc gcccggcgtc ccctggcctt tcagacggat gcgttgacca
     7321 ttgccgacgc ccgccgggat cttgacattc agcgttttcg gaatttcctg ttcgatcatg
     7381 ccaaaggcgt tataaaccgg caggttatag ctgatggtac gcttatgctc agtaagcgtt
     7441 tcttcgagga ataccgccac ttcgatttca atatcgtggc cgcgtgtggc ggggcgttga
     7501 cggctctggc gggcatgctg accgaaaatt gacgagaaga tatcgtcaaa atcttcggcg
     7561 ttaaaactct gaccgtcgct atgatggaac tgacggttaa attgcggatc gttgcgatgt
     7621 tgccacatct gatcatactc agcgcgacgt tgttcatcac ttaacacttc ccaggcttca
     7681 gcgacctctt tgaagcgggc ttcggcatcc ggttctttgc tgacatcagg atggtatttg
     7741 cgggccagtc gacgataggc ggtcttgatt gtcttgagat cgtccgtcgg tttcacgccc
     7801 atgatggcgt aataatcctt taattccata gcgttatctc gcgtaaatca acacaaattg
     7861 aaggaacccc tgtaaggtaa ctcctataag tgtagggtaa tcctcaaaat ttcatatgcc
     7921 aacacagaat atgttattga aatcatcgcg gagaggaggt cgcctcaagg tggggtgctg
     7981 aacatatttt aaacaggtga aaaagggtga gcgatttttg atagtggaac caggcactt
//
LOCUS       DAZKHF020000198         8035 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2201S31241
            SAMN43267800-rid25106413.denovo.200, whole genome shotgun sequence.
ACCESSION   DAZKHF020000198 DAZKHF020000000
VERSION     DAZKHF020000198.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267800
            Sequence Read Archive: SRR30307263
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 8035)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8035)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 8035)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350581.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350581.2
            Genome Coverage       :: 151x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:21:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,652
            CDSs (total)                      :: 5,557
            Genes (coding)                    :: 5,320
            CDSs (with protein)               :: 5,320
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 237
            CDSs (without protein)            :: 237
            Pseudo Genes (ambiguous residues) :: 0 of 237
            Pseudo Genes (frameshifted)       :: 102 of 237
            Pseudo Genes (incomplete)         :: 130 of 237
            Pseudo Genes (internal stop)      :: 52 of 237
            Pseudo Genes (multiple problems)  :: 41 of 237
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8035
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267800-rid25106413.denovo.200"
                     /serotype="O 26:H11"
                     /isolate="2201S31241"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-01"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            229..1080
                     /locus_tag="ACHOSC_RS18025"
                     /old_locus_tag="pgaptmp_003605"
     CDS             229..1080
                     /locus_tag="ACHOSC_RS18025"
                     /old_locus_tag="pgaptmp_003605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000922508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FliM/FliN family flagellar motor switch protein"
                     /protein_id="HGU4549475.1"
                     /translation="MLKYSKTPGIFKLEGNRLGRPYHRLPTLFTGNFDVIDSHLGSYF
                     LKKHRSNITLKKIGCEMDIINKNAELMVSQVGHLAFDIDRSLLLMLLGNFYGLESSLE
                     EAKAHNGLPTKTETRLKNRLALDICTLIFNLQTSGIALKLKLDSSTVITHWAYQLTFT
                     LAGDEESCFRILLDDAHTDFILNLIRHSEHSKPQQVAKSVNKPALIKEIIRSLPLTLN
                     VKIAELSMNVADLTQIKAGDILPISLGETFPVAIGQSELFSALIVEDKDKLFLSELAG
                     KNENSHE"
     gene            1073..1444
                     /locus_tag="ACHOSC_RS18030"
                     /old_locus_tag="pgaptmp_003606"
     CDS             1073..1444
                     /locus_tag="ACHOSC_RS18030"
                     /old_locus_tag="pgaptmp_003606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016233859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FliM/FliN family flagellar motor switch protein"
                     /protein_id="HGU4549476.1"
                     /translation="MSKQEDILAGDFGLTDEVAPVAKSADAETLVTRLEDRFSDSMTL
                     LKRIPVTLTLEVSSVEIMLADLLNIDDDTVIELDKLAGEPLDIKVNNILLGKAEVVVV
                     NEKYGLRVLEFNTRDINDLAP"
     gene            1441..2193
                     /gene="fliP"
                     /locus_tag="ACHOSC_RS18035"
                     /old_locus_tag="pgaptmp_003607"
     CDS             1441..2193
                     /gene="fliP"
                     /locus_tag="ACHOSC_RS18035"
                     /old_locus_tag="pgaptmp_003607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001538309.1"
                     /note="The bacterial flagellar biogenesis protein FliP
                     forms a type III secretion system (T3SS)-type pore
                     required for flagellar assembly; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar type III secretion system pore protein
                     FliP"
                     /protein_id="HGU4549477.1"
                     /translation="MKRRTQLTLGLGLLALAPVALAQGGDIALLNVVTHGNTQEYSVK
                     IQVLILMTLVGLLPTMVLMMTCFTRFIIVLSLLRQALGLQQTPPNRILIGIALSLTML
                     VMRPIWLNIYDHAVVPFENDQITLTDALSTAATPLKRFMLAQTDKKAMAQIMTIGGAK
                     GHAADQDLSIVVPAYVLSELKTAFQIGFMIYIPFLVIDLIVASVLMAMGMMMLSPLIV
                     SLPFKLMLFVLIDGWSLTIGTLTTSIRGLGLG"
     gene            2196..2468
                     /locus_tag="ACHOSC_RS18040"
                     /old_locus_tag="pgaptmp_003608"
     CDS             2196..2468
                     /locus_tag="ACHOSC_RS18040"
                     /old_locus_tag="pgaptmp_003608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001341234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0009425 - bacterial-type flagellum basal
                     body [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA];
                     GO_process: GO:0044780 - bacterial-type flagellum assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthetic protein FliQ"
                     /protein_id="HGU4549478.1"
                     /translation="MLTIDVAADIVASGIKVVILLVSVLVVPSLLVGLLVSVFQAVTQ
                     INEQTLSFLPRLIVTLVVLGVCGKWMIIQLHDLCIHLFTQAALLVH"
     gene            2470..3252
                     /gene="fliR"
                     /locus_tag="ACHOSC_RS18045"
                     /old_locus_tag="pgaptmp_003609"
     CDS             2470..3252
                     /gene="fliR"
                     /locus_tag="ACHOSC_RS18045"
                     /old_locus_tag="pgaptmp_003609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001260509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006605 - protein targeting [Evidence IEA];
                     GO_process: GO:0044780 - bacterial-type flagellum assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthetic protein FliR"
                     /protein_id="HGU4549479.1"
                     /translation="MRTSDVTQLMDLALGLWFPFVRIMAFLRYVPVLDNSALTVRVRI
                     ILSLALAIIITPLIPHPVPHDLISLNSLILTVEQILWGMLFGLMFQFLFLALQLAGQI
                     LSFNMGMSMAVMNDPSSGASTTVLAELINVYAILLFFAMDGHLLLVSVLYKGFTYWPI
                     GNALHPQTLRTIALAFSWVLASASLLALPTTFIMLIVQGCFGLLNRIAPPLNLFSLGF
                     PINMLAGLVCFATLLYNLPDHYLHLANFVLQQLDALKGHYGG"
     gene            3242..4381
                     /gene="flhB"
                     /locus_tag="ACHOSC_RS18050"
                     /old_locus_tag="pgaptmp_003610"
     CDS             3242..4381
                     /gene="flhB"
                     /locus_tag="ACHOSC_RS18050"
                     /old_locus_tag="pgaptmp_003610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016236920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA];
                     GO_process: GO:0044780 - bacterial-type flagellum assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar type III secretion system protein
                     FlhB"
                     /protein_id="HGU4549480.1"
                     /translation="MADSSSEEKTEKPSAQKLRKAREEGQLPRSKDMGLAASLFAAFV
                     VISSSFPWYADFVRESFISVHQYAQEINNPEVIGQFLRHHLLILGKFILTLLPMPAAA
                     LLSSLVPGGWLFLPKKILPDFSKISPLKGIERLFSSEHLAETGKMTVKSVVVLVMLWV
                     SLRNNFASFLGLQALPFKLAMNEGLSLYACVMRNFVILFIFFALIDVPLAKALFTKGL
                     KMTKQELKEEYKNQEGKPEVKARVRRLQRQLAMGQIRKVVPKANVVITNPTHYAVALQ
                     YDSSRAAAPFVVAKGTDEIALYIRQVAAENQVEVVEFPRLARSVYYTTQVNQQIPFQL
                     YRAIAHVLTYVLQMKHWREGAQPRPALNRHISIPKEVLKLDGENN"
     gene            4365..6458
                     /gene="flhA"
                     /locus_tag="ACHOSC_RS18055"
                     /old_locus_tag="pgaptmp_003611"
     CDS             4365..6458
                     /gene="flhA"
                     /locus_tag="ACHOSC_RS18055"
                     /old_locus_tag="pgaptmp_003611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001140831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0044780 - bacterial-type flagellum assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis protein FlhA"
                     /protein_id="HGU4549481.1"
                     /translation="MAKTTKSFLALLRGGNLGVPLVILCILAMVILPLPPALLDILFT
                     FNIVLAVMVLLVAVSAKRPLEFSLFPTILLITTLMRLTLNVASTRVVLLHGHLGADAA
                     GKVIESFGQVVIGGNFVVGFVVFIILMIINFIVVTKGAERISEVSARFTLDAMPGKQM
                     AIDADLNAGLINQAQAQTRRKDVASEADFYGAMDGASKFVRGDAIAGMMILAINLIGG
                     VCIGIFKYNLSADAAFQQYVLMTIGDGLVAQIPSLLLSTAAAIIVTRVSDNGDIAHDV
                     RHQLLASPSVLYTATGIMFVLAVVPGMPHLPFLLFSALLGFTGWRMSKRPQAAEAEEK
                     SLETLTRTITETSEQQVSWETIPLIEPISLSLGYKLVALVDKAQGNPLTQRIRGVRQV
                     ISDGNGVLLPEIRIRENFRLKPSQYAIFINGIKADEADIPADKLMALPSSETYGEIDG
                     VLGNDPAYGMPVTWIQPAQKAKALNMGYQVIDSASVIATHVNKIVRSYIPDLFNYDDI
                     TQLHNRLASMAPRLAEDLSAVLNYSQLLKVYRALLTEGVSLRDIVTIATVLVASSAVT
                     KDHILLAADVRLALRRSITHPFVRKQELTVYTLNNELENLLTNLVNQAQQGGKVMLDS
                     VPVDPNMLNQFQSTMPQVKEQMKAAGKDPVLLVPPQLRPLLARYARLFAPGLHVLSYN
                     EVPDELELKIMGALS"
     gene            6563..6841
                     /locus_tag="ACHOSC_RS18060"
                     /old_locus_tag="pgaptmp_003612"
     CDS             6563..6841
                     /locus_tag="ACHOSC_RS18060"
                     /old_locus_tag="pgaptmp_003612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000598761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003729 - mRNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicA family
                     toxin"
                     /protein_id="HGU4549482.1"
                     /translation="MIKVSALGSRHRKTFDDVMTEPPKSGIKWDDVVALIKAVGGTIK
                     NNNGSRRKFQIGTTKFSTHEPHPKNVMDKGAVAGLKEWFVNCVGVDYE"
     gene            6834..7190
                     /locus_tag="ACHOSC_RS18065"
                     /old_locus_tag="pgaptmp_003613"
     CDS             6834..7190
                     /locus_tag="ACHOSC_RS18065"
                     /old_locus_tag="pgaptmp_003613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001030479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0110001 - toxin-antitoxin complex
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicB family
                     antitoxin"
                     /protein_id="HGU4549483.1"
                     /translation="MNKASNIITIKGQPAAVTFEADINAFRGKFLNVNGYCDFVATSI
                     EALYREGESALDEWMADCEEDGIAPFREEEEQKRLTLRVPHHIDSRLTIVARQHSISK
                     NQLIVDVLEREFSAQM"
     gene            complement(7247..7951)
                     /gene="yafL"
                     /locus_tag="ACHOSC_RS18070"
                     /old_locus_tag="pgaptmp_003614"
     CDS             complement(7247..7951)
                     /gene="yafL"
                     /locus_tag="ACHOSC_RS18070"
                     /old_locus_tag="pgaptmp_003614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001382845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="C40 family peptidase"
                     /protein_id="HGU4549484.1"
                     /translation="MLCLPFSSFASATTSHITFSYAARQRMQNRARLLKQYQTHLKKQ
                     ASYIVEGNAESRRALRQHNREQIKQHPEWFPAPLKASDRRWQALAENNHFLSSDHLHN
                     ITEVAIHRLEQQLGKPYVWGGTRPDQGFDCSGLVFYAYNKILEAKLPRTANEMYHYRR
                     ATIVANNDLRRGDLLFFHIHSREIADHMGVYLGDGQFIESPRTGETIRVSQLAEPFWQ
                     DHFLGARRILTEETIL"
BASE COUNT         1808 a         2096 c         2216 g         1915 t
ORIGIN      
        1 aattgtgcac cggtgcctga tgcgatgcgg cgcgtcttat caggcctacg gttcgtgcaa
       61 tgtgttatga cgatgcctga tatctcgccc tttaataatt taattatttt ttcaccctat
      121 taataatttg cccctttcac gccccctcta tttaatacct caccttgtgt cactgccgga
      181 tttgcgtgca caaaatcgcc cataccatta cagcagagaa agtaagccat gctgaagtac
      241 agtaaaacgc caggcatctt taaattagaa ggtaatcggc tcggacgccc ctaccatcgc
      301 ctgccgaccc tgtttacagg gaattttgac gtgattgatt cgcatctggg cagctacttt
      361 ctcaaaaagc atcgcagcaa tatcacccta aaaaagattg gctgcgaaat ggatattatt
      421 aataaaaacg ctgaactgat ggtttcccag gtcgggcatc tggcgtttga tattgatcgc
      481 tctttgctgt taatgctgct gggcaatttt tatggtctgg agtcctctct tgaagaggcg
      541 aaagcgcata acgggctgcc gaccaaaacc gaaacgcggt taaaaaaccg tctggcgctg
      601 gatatctgca cgctgatttt taatctgcaa acctccggta ttgcgctgaa attaaagctg
      661 gacagcagca cggtaattac ccactgggcg tatcaattaa cctttaccct tgcgggcgat
      721 gaagagagct gttttcgtat tttgctcgac gacgcccaca ccgattttat tttaaatctg
      781 atccgccaca gcgagcacag taaaccgcag caggtggcga aatcggtcaa taaaccggcc
      841 ctgattaaag agatcatccg ctccctgccg ctgaccctga acgtcaaaat cgccgagcta
      901 tcgatgaatg tcgcggatct cacgcagata aaagccgggg acattttacc catctcgctg
      961 ggagaaacgt tcccggtggc gattgggcaa tctgaattat ttagcgccct gattgtggaa
     1021 gacaaagaca aactgttttt gtcggagctg gcgggcaaaa atgagaatag ccatgagtaa
     1081 gcaagaagat attttagccg gggattttgg cctcaccgat gaagttgcgc ctgtcgcaaa
     1141 atccgccgac gctgaaacgc tggtcacccg cctggaagac cgtttttccg actcgatgac
     1201 gctgctaaaa cgcattccgg tgaccttaac gctggaagtg tcatcagtag aaataatgtt
     1261 agccgacctc cttaacatcg acgacgacac ggtgattgag ctggataaac tcgccgggga
     1321 gccgctggat atcaaagtga acaatatcct gctgggcaaa gcggaagtgg tggtggtcaa
     1381 cgagaagtac ggcctgcgcg tgctggaatt taacacccgc gatatcaacg acctggcacc
     1441 atgaaacgcc gcactcaact gacgctgggc cttgggctgc tggcgcttgc gccggtggcg
     1501 ttggcccagg gcggcgatat tgcgctgctt aacgtggtca cccacggcaa cactcaggag
     1561 tacagcgtca aaatccaggt gctgattctg atgaccctcg tcggtttgct gcctacgatg
     1621 gtgctgatga tgacctgctt tacgcgcttt attatcgtgc tgtcgctgct gcgccaggcg
     1681 ctagggctac aacaaacgcc gccaaaccgc attctgattg gcattgcgct ctccctcacc
     1741 atgctggtga tgcgccccat ctggctcaat atttacgacc acgcagtagt gccgtttgaa
     1801 aatgatcaga ttaccctgac cgatgcgtta agcaccgccg cgacgccact caaacgcttt
     1861 atgctggcgc agactgacaa aaaagcgatg gcgcaaatca tgactatcgg cggcgcgaag
     1921 ggccatgccg ccgaccagga tctctcgatt gtggtgcccg cctatgtgtt gagcgagctg
     1981 aaaaccgcct tccagattgg ctttatgatt tacatcccgt ttctggttat cgacctgatt
     2041 gtcgccagcg tactgatggc gatggggatg atgatgctgt cgccgctgat cgtttcgcta
     2101 ccctttaagc tgatgctgtt tgtgctgatc gacggctggt cgctgaccat cggtacactc
     2161 accaccagca ttcgcgggct ggggctgggc tgatgatgct gaccatagac gtagccgccg
     2221 acattgtcgc cagcggcata aaagtggtga ttttgctggt ttcggtgctg gtagtgccga
     2281 gcctgctggt cggcctgctg gtgagcgtgt tccaggcggt aacgcagatt aacgaacaga
     2341 ccctaagctt tttgccgagg ctgattgtca cgctggtggt gctgggcgtg tgcggtaaat
     2401 ggatgattat ccagttgcat gatctctgca ttcacctgtt tacccaggcc gcgttgctgg
     2461 ttcattgaga tgcgcacaag cgacgttacg caactgatgg atctggcgct ggggctgtgg
     2521 tttccctttg tgcgcattat ggcgtttctg cgctatgtgc ctgtgctgga taacagcgcc
     2581 ctgaccgtgc gcgtgcggat tattttgtcg ctggcgctgg caatcatcat cacgccgttg
     2641 atcccccatc ctgttcccca cgacctgata tcgctcaaca gcctgatcct gacggtggag
     2701 caaattttat ggggcatgtt attcgggctg atgtttcagt ttttgtttct ggcgctacag
     2761 ctggccgggc aaattctctc gttcaatatg gggatgagca tggcggtgat gaacgacccg
     2821 agcagtggcg catcgaccac cgtgctggcg gagctcatca acgtctacgc gatcctgctg
     2881 ttttttgcca tggacgggca tctgctgctg gtgagtgtgc tgtataaggg ctttacctac
     2941 tggccgattg gcaacgcgct gcatccgcaa accctgcgca ccatcgcgct ggcctttagc
     3001 tgggtgctgg cgtccgcatc gctgctggcg ctgccaacca cttttatcat gctgattgtg
     3061 cagggctgct ttggcctgct caaccgcatt gcgccgccgc tgaacctttt ttcactcggt
     3121 tttcccatca acatgctggc ggggctggtc tgctttgcca ccctgctcta caacctgccg
     3181 gatcactatc tgcatctggc gaattttgtg ttgcagcaac tcgacgcgct gaaaggtcac
     3241 tatggcggat agcagcagcg aagaaaaaac agagaaaccc tcggcgcaaa agctgcgtaa
     3301 agcgcgcgaa gaggggcaac tgccgcgctc gaaggatatg gggctggcgg cgagcctgtt
     3361 tgcggcattt gtggtgatct ccagcagctt cccgtggtat gccgattttg tgcgcgagag
     3421 ttttatcagc gtgcaccagt atgcacagga gattaataac ccagaggtga tcggccagtt
     3481 tctgcgccat cacctgttaa ttctcggcaa gtttattctc accttgctgc cgatgcccgc
     3541 cgccgcgcta ctctcctcac tagtgcccgg cggctggctg tttctgccta aaaaaatcct
     3601 cccggatttc agcaaaatca gcccgttaaa aggcattgag cggctgtttt ccagtgaaca
     3661 tctggcggaa acgggaaaga tgacggtgaa gtcggtggtg gtgctggtga tgctgtgggt
     3721 aagcctgcgc aataactttg cctcctttct cggcctgcag gcgctaccgt tcaaactggc
     3781 gatgaatgag ggtttatcgc tctacgcctg cgtgatgcgc aactttgtca tcctgtttat
     3841 cttctttgcg ttaatcgacg tgccgctggc gaaagccctg tttaccaaag ggctgaagat
     3901 gaccaaacag gagctgaaag aagagtataa aaaccaggaa ggcaagccgg aagtgaaggc
     3961 gcgcgtgcgg cgtctgcaac ggcagttggc gatggggcaa attcgcaaag tggtgccgaa
     4021 agccaacgtg gtgatcacca acccgaccca ctacgccgtg gcgttgcagt atgactcgtc
     4081 gcgcgccgcc gcgccgtttg tggtggcaaa aggcaccgat gaaatcgccc tctatattcg
     4141 ccaggtcgcc gctgagaatc aggtggaagt ggtcgagttc ccgcgactgg cgcgctcggt
     4201 ttactacacc acccaggtga accagcaaat tccgtttcag ctttaccggg ccatcgccca
     4261 cgttctgacc tacgtcctgc aaatgaaaca ctggcgcgag ggtgcacagc cgcgcccggc
     4321 cctgaacaga catatttcca ttcccaaaga ggtgcttaaa ctggatggcg aaaacaacta
     4381 aatcgttcct cgcgctgctg cgcggcggca atcttggcgt gccgctggtg atcctgtgta
     4441 ttctggcgat ggttattttg ccgctgccgc ctgcgctgct ggatattctg ttcaccttca
     4501 acattgtgct ggcggtgatg gtgctgctgg tggcggtgtc ggcgaaacga ccgctggaat
     4561 tcagcctgtt cccgaccatt ttgctgatca ccaccttaat gcgcctgacg ctcaacgtcg
     4621 cctctacgcg cgtggtgctg ctgcacgggc atctcggcgc ggacgcggcg ggtaaggtga
     4681 ttgagtcgtt tggtcaggtg gtgatcggcg gcaactttgt tgtcggcttt gtggtgttta
     4741 tcatcctgat gatcatcaac tttattgtcg tcaccaaagg ggccgagcgt atttccgagg
     4801 tttcagcccg ctttaccctg gatgcgatgc ccggtaagca gatggcgatt gacgccgatc
     4861 ttaatgccgg gttgatcaat caggcgcagg cacaaacccg acgtaaagat gttgccagcg
     4921 aggccgattt ctacggcgcg atggacgggg catcgaaatt tgtgcgcggg gatgctatcg
     4981 ccgggatgat gattctggcg atcaacctga tcggcggcgt ctgtatcggg atcttcaaat
     5041 acaacctgag cgccgacgcc gccttccagc agtatgtgct gatgaccatc ggcgacgggc
     5101 tggtggcgca aatcccttcc ctgctactct ccaccgcggc ggcgattatc gtcacccgcg
     5161 tcagcgacaa cggcgatatc gcccatgacg tgcgtcacca gttgctggca agcccatcgg
     5221 tgctctacac cgctaccgga attatgttcg tactggcggt agtgcctgga atgccacatc
     5281 tgccgttttt gctgttcagc gccctgcttg gctttaccgg ctggcggatg agcaaacgcc
     5341 cgcaggcggc ggaagcggaa gagaaaagtc tcgaaacgct gacccgcacc atcaccgaaa
     5401 ccagcgagca acaggtcagt tgggaaacca ttccgctgat cgagcccatc agcttaagcc
     5461 tcggctacaa gctggtggcg ctggtggaca aagcccaggg caacccgctc acccagcgta
     5521 ttcgcggcgt gcgacaggtg atatccgacg gcaacggcgt gctgctgccg gagatccgca
     5581 ttcgggaaaa cttccgcctt aagcccagcc agtacgctat tttcatcaac ggcattaagg
     5641 ctgatgaagc ggatattccg gcggataaac tgatggcgct gccctccagc gaaacctacg
     5701 gcgagataga cggcgtgctg gggaacgacc cggcgtacgg gatgccggtg acctggatcc
     5761 agcctgcgca gaaagcgaag gcgctgaata tggggtatca ggtgatcgac agcgccagcg
     5821 tgattgctac ccatgtaaat aagattgtgc gcagctatat tcctgattta tttaactatg
     5881 acgatattac gcagttgcat aaccgtttgg cgtcgatggc accgcgcctg gcggaggatt
     5941 taagcgcggt gctcaattac agccagttgc tgaaagtgta ccgggcgctg ctgaccgaag
     6001 gcgtttccct gcgcgatatc gtcaccatcg ccaccgtgct ggtcgccagt agcgcggtga
     6061 ccaaagatca tattctgctg gcagccgatg tgcgcctggc gctgcggcgc agcattaccc
     6121 atccgttcgt tcgcaagcag gagctgacgg tgtataccct gaataatgag ctggaaaatc
     6181 tgctgactaa cctagtgaat caggcgcaac agggcggtaa agtgatgctc gacagcgtgc
     6241 cagtggaccc gaatatgctc aaccagttcc agagtacgat gccacaggtg aaagagcaga
     6301 tgaaagcggc ggggaaagac ccggtgctgc tggtgccacc gcagctgcgc cctttgctgg
     6361 cgcgttatgc aaggttgttt gcgccggggc tgcatgtgct gtcgtataac gaagtgccgg
     6421 atgagctgga gttgaagatt atgggggcgt tgagttgatg ggggattctt ttccatgctt
     6481 tgcagcacct gacaccatta attgacgtca cactgcaaag agactacgat gacaccatac
     6541 aatgacgtca tggattgata gtatgattaa ggtcagcgcg ctaggttctc gtcatagaaa
     6601 aacattcgac gatgtaatga cggagccgcc aaaatctgga attaaatggg atgatgttgt
     6661 tgcactcatt aaagctgtgg gtggaacaat aaaaaataac aacggctcca gacgaaaatt
     6721 tcagattgga acgaccaaat tcagcacaca tgagccacat ccaaaaaatg tgatggataa
     6781 aggcgcagta gcaggtctta aggagtggtt tgttaactgt gtaggggtag attatgaata
     6841 aagcatcgaa cattatcaca attaaaggtc aaccagccgc agtaacattt gaagccgata
     6901 tcaacgcatt tcgcggcaag ttcctcaatg taaacggtta ttgtgacttt gtagccacaa
     6961 gtattgaggc attatatcgt gaaggcgaaa gcgcgctgga tgagtggatg gcggattgtg
     7021 aagaggacgg tattgcccct tttcgtgaag aagaagagca aaaacgcctg acgcttcgtg
     7081 ttcctcatca cattgattcg cggctgacca tcgtcgccag acaacattcc atttcaaaaa
     7141 accagctcat tgttgatgtc ttagaacgtg agttttcagc ccagatgtaa acaggtcgga
     7201 tgccgcgtgg catccgacct tttgccagtt cgtcaatatt acccatttac aaaatcgtct
     7261 cctccgtcaa aatccgccgc gcgcctaaaa agtgatcctg ccagaaaggt tcagctaact
     7321 gacttacccg aatggtttcc ccggtgcgcg gtgactcaat aaactgaccg tcgcccagat
     7381 acacccccat atggtcggca atctcgcggc tgtggatatg gaaaaatagc aaatctcccc
     7441 ggcgcaggtc gttattcgcc acaatggttg cccggcgata gtgatacatc tcattagccg
     7501 tgcgtgggag tttcgcctca aggatcttgt tgtaggcata aaaaactaac ccgctacagt
     7561 cgaagccttg atcgggccgc gtaccgcccc agacgtaagg cttgccaagc tgttgctcca
     7621 gacggtgaat cgccacttcg gtgatgttat gcagatggtc gctgcttaag aaatggttgt
     7681 tttccgccag cgcctgccag cgtctgtcac tcgccttgag cggcgcagga aaccattctg
     7741 gatgctgttt tatctgttcg cggttgtgct ggcgtagcgc ccttctgctt tcggcattgc
     7801 cttccacaat atagctggcc tgttttttca gatgggtttg gtactgtttt aataaacgcg
     7861 cacgattttg catccgctgg cgggcggcgt agctgaaagt gatatgtgat gtggtggcgc
     7921 tggcaaatga agaaaacggc aagcaaagca acagtaatcc cgacaacaca aaagagggaa
     7981 tcgacggcaa ggacatactc aataatggct gatcatgcat tgatgttgca gtgta
//
LOCUS       DAZKHF020000199         7938 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2201S31241
            SAMN43267800-rid25106413.denovo.201, whole genome shotgun sequence.
ACCESSION   DAZKHF020000199 DAZKHF020000000
VERSION     DAZKHF020000199.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267800
            Sequence Read Archive: SRR30307263
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 7938)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7938)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 7938)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350581.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350581.2
            Genome Coverage       :: 151x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:21:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,652
            CDSs (total)                      :: 5,557
            Genes (coding)                    :: 5,320
            CDSs (with protein)               :: 5,320
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 237
            CDSs (without protein)            :: 237
            Pseudo Genes (ambiguous residues) :: 0 of 237
            Pseudo Genes (frameshifted)       :: 102 of 237
            Pseudo Genes (incomplete)         :: 130 of 237
            Pseudo Genes (internal stop)      :: 52 of 237
            Pseudo Genes (multiple problems)  :: 41 of 237
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7938
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267800-rid25106413.denovo.201"
                     /serotype="O 26:H11"
                     /isolate="2201S31241"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-01"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            complement(176..319)
                     /gene="ypaB"
                     /locus_tag="ACHOSC_RS18075"
                     /old_locus_tag="pgaptmp_003615"
     CDS             complement(176..319)
                     /gene="ypaB"
                     /locus_tag="ACHOSC_RS18075"
                     /old_locus_tag="pgaptmp_003615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001753443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YpaB"
                     /protein_id="HGU4549485.1"
                     /translation="MALLQVHNFVDNSGRKKWLSRTLGQTRCPGKSMGREKFVKNNCS
                     AIS"
     gene            447..2732
                     /gene="nrdA"
                     /locus_tag="ACHOSC_RS18080"
                     /old_locus_tag="pgaptmp_003616"
     CDS             447..2732
                     /gene="nrdA"
                     /locus_tag="ACHOSC_RS18080"
                     /old_locus_tag="pgaptmp_003616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004748 - ribonucleoside-diphosphate
                     reductase activity, thioredoxin disulfide as acceptor
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class 1a ribonucleoside-diphosphate reductase
                     subunit alpha"
                     /protein_id="HGU4549486.1"
                     /translation="MNQNLLVTKRDGSTERINLDKIHRVLDWAAEGLHNVSISQVELR
                     SHIQFYDGIKTSDIHETIIKAAADLISRDAPDYQYLAARLAIFHLRKKAYGQFEPPAL
                     YDHVVKMVEMGKYDNHLLEDYTEEEFKQMDTFIDHDRDMTFSYAAVKQLEGKYLVQNR
                     VTGEIYESAQFLYILVAACLFSNYPRETRLQYVKRFYDAVSTFKISLPTPIMSGVRTP
                     TRQFSSCVLIECGDSLDSINATSSAIVKYVSQRAGIGINAGRIRALGSPIRGGEAFHT
                     GCIPFYKHFQTAVKSCSQGGVRGGAATLFYPMWHLEVESLLVLKNNRGVEGNRVRHMD
                     YGVQINKLMYTRLLKGEDITLFSPSDVPGLYDAFFADQEEFERLYTKYEKDDSIRKQR
                     VKAVELFSLMMQERASTGRIYIQNVDHCNTHSPFDPAIAPVRQSNLCLEIALPTKPLN
                     DVNDENGEIALCTLSAFNLGAINSLDELEELAILAVRALDALLDYQDYPIPAAKRGAM
                     GRRTLGIGVINFAYYLAKHGVRYSDGSANNLTHKTFEAIQYYLLKASNELAKEQGACP
                     WFNETTYAKGILPIDTYKKDLDTIANEPLHYDWEALRESIKTHGLRNSTLSALMPSET
                     SSQISNATNGIEPPRGYVSIKASKDGILRQVVPDYEHLHDAYELLWEMPGNDGYLQLV
                     GIMQKFIDQSISANTNYDPSRFPSGKVPMQQLLKDLLTAYKFGVKTLYYQNTRDGAED
                     AQDDLVPSIQDDGCESGACKI"
     gene            2821..3951
                     /gene="nrdB"
                     /locus_tag="ACHOSC_RS18085"
                     /old_locus_tag="pgaptmp_003617"
     CDS             2821..3951
                     /gene="nrdB"
                     /locus_tag="ACHOSC_RS18085"
                     /old_locus_tag="pgaptmp_003617"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006683700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0009263 - deoxyribonucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class Ia ribonucleoside-diphosphate reductase
                     subunit beta"
                     /protein_id="HGU4549487.1"
                     /translation="MAYTTFSQTKNDQLKEPMFFGQPVNVARYDQQKYDIFEKLIEKQ
                     LSFFWRPEEVDVSRDRIDYQALPEHEKHIFISNLKYQTLLDSIQGRSPNVALLPLISI
                     PELETWVETWAFSETIHSRSYTHIIRNIVNDPSVVFDDIVTNEQIQKRAEGISSYYDE
                     LIEMTSYWHLLGEGTHTVNGKTVTVSLRELKKKLYLCLMSVNALEAIRFYVSFACSFA
                     FAERELMEGNAKIIRLIARDEALHLTGTQHMLNLLRSGADDPEMAEIAEECKQECYDL
                     FVQAAQQEKDWADYLFRDGSMIGLNKDILCQYVEYITNIRMQAVGLDLPFQTRSNPIP
                     WINTWLVSDNVQVAPQEVEVSSYLVGQIDAEVDTDDLSNFQL"
     gene            3951..4205
                     /gene="yfaE"
                     /locus_tag="ACHOSC_RS18090"
                     /old_locus_tag="pgaptmp_003618"
     CDS             3951..4205
                     /gene="yfaE"
                     /locus_tag="ACHOSC_RS18090"
                     /old_locus_tag="pgaptmp_003618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin-like diferric-tyrosyl radical
                     cofactor maintenance protein YfaE"
                     /protein_id="HGU4549488.1"
                     /translation="MARVTLRITGTQLLCQDEHPSLLAALESHNVAVEYQCREGYCGS
                     CRTRLVAGQVDWIAEPLAFIQPGEILPCCCRAKGDIEIEM"
     gene            complement(4259..4909)
                     /gene="inaA"
                     /locus_tag="ACHOSC_RS18095"
                     /old_locus_tag="pgaptmp_003619"
     CDS             complement(4259..4909)
                     /gene="inaA"
                     /locus_tag="ACHOSC_RS18095"
                     /old_locus_tag="pgaptmp_003619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide kinase InaA"
                     /protein_id="HGU4549489.1"
                     /translation="MAVSAKYDEFNHWWATEGDWVEEPNYRRNGMSGVQCVERNGKKL
                     YVKRMTHHLFHSVRYPFGRPTIVREVAVIKELERAGVIVPKIVFGEAVKIEGEWRALL
                     VTEDMAGFISIADWYAQHAVSPYSDEVRQAMLKAVALAFKKMHSINRQHGCCYVRHIY
                     VKTEGNAEAGFLDLEKSRRRLRRDKAINHDFRQLEKYLEPIPKADWEQVKAYYYAM"
     gene            complement(4989..5093)
                     /locus_tag="ACHOSC_RS18100"
                     /old_locus_tag="pgaptmp_003620"
                     /pseudo
     CDS             complement(4989..5093)
                     /locus_tag="ACHOSC_RS18100"
                     /old_locus_tag="pgaptmp_003620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311148.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            5091..5330
                     /locus_tag="ACHOSC_RS18105"
                     /old_locus_tag="pgaptmp_003621"
                     /pseudo
     CDS             5091..5330
                     /locus_tag="ACHOSC_RS18105"
                     /old_locus_tag="pgaptmp_003621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311149.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
     gene            complement(5372..6448)
                     /gene="glpQ"
                     /locus_tag="ACHOSC_RS18110"
                     /old_locus_tag="pgaptmp_003622"
     CDS             complement(5372..6448)
                     /gene="glpQ"
                     /locus_tag="ACHOSC_RS18110"
                     /old_locus_tag="pgaptmp_003622"
                     /EC_number="3.1.4.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008081 - phosphoric diester hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase"
                     /protein_id="HGU4549490.1"
                     /translation="MKLTLKNLSMAIMMSTIVMGSSAMAADSNEKIVIAHRGASGYLP
                     EHTLPAKAMAYAQGADYLEQDLVMTKDDHLVVLHDHYLDRVTDVADRFPDRARKDGRY
                     YAIDFTLDEIKSLKFTEGFDIENGKKVQTYPGRFPMGKSDFRVHTFEEEIEFVQGLNH
                     STGKNIGIYPEIKAPWFHHQEGKDIAAKTLEVLKKYGYTGKDDKVYLQCFDADELKRI
                     KNELEPKMGMDLNLVQLIAYTDWNETQQKQPDGSWVNYNYDWMFKPGAMKQVAEYADG
                     IGPDYHMLIEETSQPGNIKLTGMVQDAQQNKLVVHPYTVRSDKLPEYTTDVNQLYDAL
                     YNKAGVNGLFTDFPDKAVKFLNKE"
     gene            complement(6453..7811)
                     /gene="glpT"
                     /locus_tag="ACHOSC_RS18115"
                     /old_locus_tag="pgaptmp_003623"
     CDS             complement(6453..7811)
                     /gene="glpT"
                     /locus_tag="ACHOSC_RS18115"
                     /old_locus_tag="pgaptmp_003623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015169 - glycerol-3-phosphate
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0015794 - glycerol-3-phosphate
                     transmembrane transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate transporter"
                     /protein_id="HGU4549491.1"
                     /translation="MLSIFKPAPHKARLPAAEIDPTYRRLRWQIFLGIFFGYAAYYLV
                     RKNFALAMPYLVEQGFSRGDLGFALSGISIAYGFSKFIMGSVSDRSNPRVFLPAGLIL
                     AAAVMLFMGFVPWATSSIAVMFVLLFLCGWFQGMGWPPCGRTMVHWWSQKERGGIVSV
                     WNCAHNVGGGIPPLLFLLGMAWFNDWHAALYMPAFCAILVALFAFAMMRDTPQSCGLP
                     PIEEYKNDYPDDYNEKAEQELTAKQIFMQYVLPNKLLWYIAIANVFVYLLRYGILDWS
                     PTYLKEVKHFALDKSSWAYFLYEYAGIPGTLLCGWMSDKVFRGNRGATGVFFMTLVTI
                     ATIVYWMNPAGNPTVDMICMIVIGFLIYGPVMLIGLHALELAPKKAAGTAAGFTGLFG
                     YLGGSVAASAIVGYTVDFFGWDGGFMVMIGGSILAVILLIVVMIGEKRRHEQLLQKRN
                     GG"
BASE COUNT         2045 a         2138 c         1849 g         1906 t
ORIGIN      
        1 ataaccgaat agaaaacaac catttcgaca tcaacaatcg cttacattcg cttatatatt
       61 gaccacaact gatacatcag attatgtgat gactcgtgct tagatcaatt tttgcaatca
      121 ttagcaaaaa gattaataaa ccatctatat caatttatcc aacctattct gccgttcaag
      181 aaatcgccga acagttattt ttaacaaatt tttctcttcc cattgacttt cccggacacc
      241 ttgtctgacc taaggtgcgc gaaagccact ttttccttcc tgagttatcc acaaagttat
      301 gcacttgcaa gagggccatt ttcacactat cttgcagtga atcccaaaca taccccctat
      361 atatagtgtt ctaagcagct tcccgtacta caggtagtct gcatgaaact attgcggaaa
      421 gaattccaaa aacaggtacg acatacatga atcagaatct gctggtgaca aagcgcgacg
      481 gtagcacaga gcgcatcaat ctcgacaaaa tccatcgcgt tctggattgg gcggcagaag
      541 gactgcataa cgtttcgatt tcccaggtcg agctgcgctc ccacattcag ttttatgacg
      601 gtatcaagac ctctgacatc cacgaaacca ttatcaaggc ggccgcagac ctgatctccc
      661 gtgatgcgcc ggattatcag tatctcgccg cgcgcctggc gatcttccac ctgcgtaaaa
      721 aagcctacgg ccagtttgag ccgcctgcgc tgtacgacca cgtggtgaaa atggtcgaga
      781 tgggcaaata cgataatcat ctgctggaag actacacgga agaagagttc aagcagatgg
      841 acacgtttat cgatcacgac cgtgatatga ccttctctta tgctgccgtt aagcagctgg
      901 aaggcaaata tctggtacag aaccgcgtga ccggcgaaat ctacgaaagc gcccagttcc
      961 tttatattct ggttgccgcg tgcctgttct cgaactaccc gcgtgaaacg cgcctgcaat
     1021 atgtgaagcg tttttacgac gcggtttcca catttaaaat ttcgctgccg acgccaatca
     1081 tgtccggcgt gcgtaccccg actcgtcagt tcagctcctg cgtactgatc gagtgcggtg
     1141 acagtctgga ttccatcaac gctacctcca gcgcgattgt gaaatacgtt tcccagcgtg
     1201 ccgggatcgg catcaacgcc gggcgtattc gtgcgctggg tagcccgatt cgcggtggtg
     1261 aagcgttcca taccggctgt attccgttct acaaacattt ccagacagcg gtgaaatcct
     1321 gctctcaggg cggcgtgcgc ggcggtgcgg caacgctgtt ctacccgatg tggcatctgg
     1381 aagtggaaag cctgctggtg ttgaaaaaca accgtggtgt ggaaggcaac cgcgtgcgcc
     1441 atatggacta cggggtacag atcaacaaac tgatgtatac ccgcctgctg aaaggtgaag
     1501 atatcaccct gttcagcccg tccgacgtac cggggctgta cgacgcattc ttcgccgatc
     1561 aggaagagtt tgaacgcctg tataccaaat atgagaaaga cgacagcatc cgcaagcagc
     1621 gtgtgaaagc cgttgagctg ttctcgctga tgatgcagga acgtgcgtct accggtcgta
     1681 tctatattca gaacgttgac cactgcaata cccatagccc gtttgatccg gccatcgcgc
     1741 cagtgcgtca gtctaacctg tgcctggaga tagccctgcc gaccaaaccg ctgaacgacg
     1801 tcaacgacga gaacggtgaa atcgcgctgt gtacgctgtc tgctttcaac ctgggcgcaa
     1861 ttaatagcct ggatgaactg gaagagctgg caattctggc ggttcgtgca cttgacgcgc
     1921 tgctggatta tcaggattac ccgatcccgg ccgccaaacg tggagcgatg ggtcgtcgta
     1981 cgctgggtat tggtgtgatc aacttcgctt actacctggc gaagcacggt gtgcgttact
     2041 ccgacggcag cgccaacaac ctgacgcata aaaccttcga agcgattcag tattacctgc
     2101 tgaaagcgtc taacgagctg gcaaaagagc aaggcgcgtg cccgtggttt aacgaaacca
     2161 cttacgcgaa agggatcctg ccgatcgata cctataagaa agatctggat accatcgcta
     2221 atgagccgct gcattacgac tgggaagcat tgcgtgaatc aatcaaaacg cacggcctgc
     2281 gtaactccac gctttctgcc ctgatgccgt cagagacttc ttcacagatc tctaacgcca
     2341 ctaacggtat tgaaccgccg cgcggttacg tcagcatcaa agcgtcgaaa gacggtattt
     2401 tgcgccaggt ggtgccggac tacgagcacc tgcacgacgc ctatgagctg ctgtgggaaa
     2461 tgccgggtaa cgatggctac ctgcaactgg taggtatcat gcagaaattt atcgatcagt
     2521 cgatctctgc taacaccaac tacgatccgt cacgtttccc gtcaggaaaa gtgccgatgc
     2581 agcagttgct gaaagacctg ctcaccgcct acaaattcgg ggtcaaaacg ctgtattatc
     2641 agaacacccg tgacggcgct gaagacgcgc aggacgatct ggtaccgtca atccaggacg
     2701 atggctgcga aagcggcgca tgtaagatct gataatcccc cagcctttgc accaaaccgt
     2761 cccctctccc cttgcgggag agggttaggg tgaggggtaa taaaaccaca ggacacactc
     2821 atggcatata ccaccttttc acagacgaaa aatgatcagc tcaaagaacc gatgttcttt
     2881 ggtcagccgg tcaacgtggc tcgctacgat cagcaaaaat atgacatctt cgaaaagctg
     2941 atcgaaaagc agctctcttt cttctggcgt ccggaagaag ttgacgtttc tcgcgaccgt
     3001 atcgattacc aggcactgcc tgaacacgaa aaacacatct ttatcagcaa cctgaaatat
     3061 cagacgctgc tggattccat tcagggccgt agcccgaacg tggcactgct gccgctgatc
     3121 tcgatcccgg aactggagac ctgggtcgaa acctgggcgt tctcagaaac gattcattcc
     3181 cgttcctata ctcatatcat tcgtaatatc gttaacgatc cgtctgttgt gtttgacgat
     3241 atcgtcacca acgagcagat ccagaaacgt gcggaaggga tctccagcta ttacgatgag
     3301 ctgatcgaaa tgaccagcta ctggcatctg ctgggcgaag gtacccacac cgttaacggt
     3361 aaaactgtga ccgttagcct gcgcgagctg aagaaaaaac tgtatctctg cctgatgagc
     3421 gttaacgcgc tggaagcgat tcgtttctac gtcagctttg cttgttcctt cgcatttgca
     3481 gaacgcgaat tgatggaagg caacgccaaa attattcgcc tgattgcccg cgacgaagcc
     3541 ctgcacctga ccggcaccca gcatatgctg aatctgctgc gcagcggcgc ggacgatcct
     3601 gagatggcgg aaattgccga agagtgtaag caggagtgct atgacctgtt tgttcaggca
     3661 gctcaacagg agaaagactg ggcggattat ctgttccgcg acggttcgat gattggtctg
     3721 aataaagaca ttctctgcca gtacgttgaa tacatcacca atatccgtat gcaggcagtc
     3781 ggtttggatc tgccgttcca gacgcgctcc aacccgatcc catggatcaa cacctggctg
     3841 gtgtctgata acgtgcaggt tgctccgcag gaagtggaag tcagttctta tctggtcggg
     3901 cagattgacg cggaagtgga caccgacgat ttgagtaact tccaactctg atggcccgcg
     3961 ttaccctgcg catcactggc acacaactgc tgtgccagga tgaacaccct tcccttctgg
     4021 cggcgctgga atcccacaat gtggcggttg agtatcagtg tcgcgaaggt tactgcggct
     4081 cctgtcgcac ccgtctggtt gcaggtcagg ttgactggat tgccgaaccg ttagccttta
     4141 ttcagccggg ggaaattttg ccctgttgtt gccgggcaaa aggcgatatt gaaatcgaga
     4201 tgtgaattgg ttgtagtgcc agatacaacg cttatgcgtc ttatctggcc tacaacgatt
     4261 acatggcgta gtaatacgct ttcacctgct cccagtccgc tttggggatt ggctccagat
     4321 atttttccag ctggcggaag tcatgattaa tcgctttatc gcgacgcaag cggcgacgac
     4381 ttttctccag gtcaagaaaa ccagcttctg cattaccttc tgttttcaca tagatatggc
     4441 gaacataaca acagccatgc tgacgattaa tgctatgcat tttcttaaac gccagcgcca
     4501 ctgctttcaa catggcttgc cgtacttcgt cagaataagg cgatactgca tgctgggcat
     4561 accagtcagc aatgctgatg aaccccgcca tatcttcagt caccagcaac gctcgccatt
     4621 caccctcaat tttcaccgct tcaccaaaaa cgatcttcgg cacgatgaca ccggcccgtt
     4681 ccagttcttt aattaccgca acctcacgga caatcgttgg tcggccgaac ggataacgta
     4741 cggaatgaaa cagatgatgc gtcatgcgct ttacatacag ctttttgccg ttgcgctcga
     4801 cgcattgcac cccgctcata ccattacggc gatagttagg ctcttcaacc cagtcgccct
     4861 ctgttgccca ccagtgatta aattcgtcgt actttgctga aactgccata ccctatcgcc
     4921 tgtcattttt attaacgaca atgactatag gtggttacct gaggaaaatc ttaatgaaac
     4981 gtgtcgtatt aatgaatttc ttttgccact actagcgtga caccgctttt acccttcatt
     5041 tgcagcgcat agattccacc agccactacc actaatagtg cgcccagtag ataaaatgtg
     5101 aatttaatca gtcggataca gcgatgaagt ttattcgaca gggattaggc atcgccttac
     5161 aaccagagtt aacgctgaaa agcattgcag gtgaattgtg ttccgttcct cacgaaccaa
     5221 ccttctatcg acagatttcg ttgttggcta aagaaaagcc ggtagaaggc agtccactgt
     5281 ttttactaca aacgtgtacg gaacaactcg tggtgaatgg aaaaatttga tgtgagacag
     5341 gatggcgcat gaacgccatc ctgcagtagc attactcttt attaaggaat tttactgcct
     5401 tatcagggaa atcagtaaac agcccattta cacccgcttt gttatacaga gcatcatata
     5461 actgattcac atcagtagtg tattcaggca gtttatctga ccgcacggta taaggatgca
     5521 ctaccagttt attctgctga gcatcttgca ccatgccagt gagtttgata ttacccggct
     5581 gcgatgtttc ctcaatcaac atatggtaat ccggcccgat tccgtcggca tattccgcca
     5641 cctgtttcat agcacccggc ttaaacatcc agtcgtagtt gtaattaacc cagcttccat
     5701 ccggctgttt ctgctgcgtt tcattccagt cggtataggc aatcaactgt accagattga
     5761 gatccatgcc cattttaggt tccagctcat tcttaatacg cttcagctca tcagcatcaa
     5821 aacattgcaa ataaactttg tcgtccttac cggtgtaacc atatttcttc agcacttcca
     5881 gcgtttttgc cgcaatatcc ttcccttcct gatgatggaa ccacggcgct ttgatttcag
     5941 gataaatacc gatatttttc ccggtagagt gatttaaccc ctgaacaaat tcaatctctt
     6001 cttcaaaggt gtgcacccgg aagtcggact tacccattgg gaaacgcccc ggataagtct
     6061 gcactttttt accgttttca atatcgaaac cttcggtaaa tttcagcgac ttaatttcat
     6121 ccagcgtgaa atctatcgcg tagtaacgac cgtctttgcg cgcccggtcc gggaaacgat
     6181 cggcaacatc agtaacacga tcgagataat ggtcatgcag aacaaccaga tggtcgtctt
     6241 tggtcatcac caaatcctgt tccagataat ccgctccctg cgcatacgcc atcgcttttg
     6301 ctggcagcgt atgctccggc aaatatccac tggcaccgcg atgggcgatg actatttttt
     6361 cgttgctgtc cgccgccatt gcactgcttc ccatgactat agtgctcatc atgatcgcca
     6421 tgctaaggtt tttcagcgtc aatttcatgc cattagcctc cgttgcgttt ttgcagtaat
     6481 tgttcatggc gacgtttttc gccaatcatc acaacaatca acaagataac cgccagaatg
     6541 ctgccgccaa tcattaccat aaagccgcca tcccagccga agaagtccac ggtgtagcca
     6601 acaatcgcgc tcgccgccac cgaaccgccc aggtaaccaa acagcccggt aaagcccgct
     6661 gccgtacctg ccgctttttt cggtgccagt tccagcgcat gcagaccgat cagcatcaca
     6721 ggaccgtaga tcaggaagcc gataacaatc atacaaatca tatcgacggt tgggttacct
     6781 gccgggttca tccagtaaac gatagtcgcg atggtcacca gtgtcataaa gaaaacgccg
     6841 gttgccccac ggttgccacg gaagacttta tccgacatcc agccgcacag cagagtgccc
     6901 ggaatacctg catattcata aaggaagtag gcccaggagg atttatccag cgcgaaatgc
     6961 ttaacctctt tcagataagt cggtgaccag tcgaggatgc cgtaacgcag cagataaacg
     7021 aacacgttgg cgatggcgat ataccacagc agtttgttcg gcagcacgta ctgcatgaag
     7081 atttgcttcg cggtcagctc ctgttccgct ttttcgttat agtcgtccgg ataatcattt
     7141 ttgtactctt cgatcggcgg caagccacag gattgcgggg tatcgcgcat catcgcaaag
     7201 gcgaataatg ccaccagaat ggcgcagaaa gcaggcatat agagcgccgc atgccagtca
     7261 ttgaaccagg ccatccccag caggaacagc agcggcggaa taccaccacc gacgttgtgc
     7321 gcacagttcc acactgacac aatgccgcca cgttctttct gcgaccacca gtgcaccata
     7381 gtacgaccac acggcggcca ccccatcccc tggaaccaac cgcagaggaa caacagtaca
     7441 aacatcaccg caatgctcga cgtcgcccat ggcacaaagc ccataaacaa catcactgcc
     7501 gccgccagaa tcaaacctgc gggcaggaaa acgcgcggat tcgagcgatc cgataccgaa
     7561 cccattatga atttcgaaaa tccataagcg atcgagatcc ccgaaagcgc gaaacctaaa
     7621 tcaccgcgag agaatccctg ctcgaccaga taaggcatag caagcgcaaa gttcttacga
     7681 accaaatagt aagccgcata gccaaagaat atccccagga aaatttgcca gcgcaatcga
     7741 cgataagtcg gatcgatctc cgcagcaggt aagcgcgctt tgtgtggcgc tggtttaaaa
     7801 atactcaaca ttgaaagcct ccgtggcccg tggtcttatt tatgattaac agactgattc
     7861 agtgggagaa cctgccgttt ctttagttgc cgcgatgtta agaaaacatt cagaaattaa
     7921 atgtgaattg ccgcacac
//
LOCUS       DAZKHF020000200         7910 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2201S31241
            SAMN43267800-rid25106413.denovo.202, whole genome shotgun sequence.
ACCESSION   DAZKHF020000200 DAZKHF020000000
VERSION     DAZKHF020000200.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267800
            Sequence Read Archive: SRR30307263
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 7910)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7910)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 7910)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350581.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350581.2
            Genome Coverage       :: 151x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:21:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,652
            CDSs (total)                      :: 5,557
            Genes (coding)                    :: 5,320
            CDSs (with protein)               :: 5,320
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 237
            CDSs (without protein)            :: 237
            Pseudo Genes (ambiguous residues) :: 0 of 237
            Pseudo Genes (frameshifted)       :: 102 of 237
            Pseudo Genes (incomplete)         :: 130 of 237
            Pseudo Genes (internal stop)      :: 52 of 237
            Pseudo Genes (multiple problems)  :: 41 of 237
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7910
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267800-rid25106413.denovo.202"
                     /serotype="O 26:H11"
                     /isolate="2201S31241"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-01"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            complement(93..323)
                     /locus_tag="ACHOSC_RS18120"
                     /old_locus_tag="pgaptmp_003624"
     CDS             complement(93..323)
                     /locus_tag="ACHOSC_RS18120"
                     /old_locus_tag="pgaptmp_003624"
                     /inference="COORDINATES: protein motif:HMM:NF015365.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS1 family transposase"
                     /protein_id="HGU4549492.1"
                     /translation="MNCWRCFPLFFIGILTTDEWGSHIRYVIEGDSSIFIQHIECMRI
                     LIKHLTRRAICFPHSVNFIKKHRYFIEKYMFY"
     gene            246..738
                     /locus_tag="ACHOSC_RS18125"
                     /old_locus_tag="pgaptmp_003625"
                     /pseudo
     CDS             246..738
                     /locus_tag="ACHOSC_RS18125"
                     /old_locus_tag="pgaptmp_003625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_086558155.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(805..933)
                     /locus_tag="ACHOSC_RS18130"
                     /old_locus_tag="pgaptmp_003626"
     CDS             complement(805..933)
                     /locus_tag="ACHOSC_RS18130"
                     /old_locus_tag="pgaptmp_003626"
                     /inference="COORDINATES: protein motif:HMM:NF025189.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase domain-containing protein"
                     /protein_id="HGU4549493.1"
                     /translation="MFFGSDHGGERGALLYGLIGTCRLNGIDPEAYLRHILSVLPE"
     gene            complement(964..1730)
                     /locus_tag="ACHOSC_RS18135"
                     /old_locus_tag="pgaptmp_003627"
                     /pseudo
     CDS             complement(964..1730)
                     /locus_tag="ACHOSC_RS18135"
                     /old_locus_tag="pgaptmp_003627"
                     /inference="COORDINATES: protein motif:HMM:NF015038.4"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family transposase"
     gene            complement(1766..2305)
                     /locus_tag="ACHOSC_RS18140"
                     /old_locus_tag="pgaptmp_003628"
     CDS             complement(1766..2305)
                     /locus_tag="ACHOSC_RS18140"
                     /old_locus_tag="pgaptmp_003628"
                     /inference="COORDINATES: protein motif:HMM:NF024408.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family transposase"
                     /protein_id="HGU4549494.1"
                     /translation="MLTTSESQFRQKDQQLSLVEETEAFLCSALARAEEKIEEDEREI
                     EHLQTQIEKLRRMLFGTRSEKLRREVEQAEALLKQREQDSDRYSGREDAPQVPRQLRQ
                     PRHRRPLPAHLPHAIHRLESEESCCPECGGELDYLEEVSAEQLELVSSALKVIRTVRV
                     KKPVQNVTASLKHRRRPAR"
     gene            complement(2317..3469)
                     /locus_tag="ACHOSC_RS18145"
                     /old_locus_tag="pgaptmp_003629"
                     /pseudo
     CDS             complement(2317..3469)
                     /locus_tag="ACHOSC_RS18145"
                     /old_locus_tag="pgaptmp_003629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611553.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3-like element IS911 family transposase"
     gene            complement(3450..3926)
                     /locus_tag="ACHOSC_RS18150"
                     /old_locus_tag="pgaptmp_003630"
     CDS             complement(3450..3926)
                     /locus_tag="ACHOSC_RS18150"
                     /old_locus_tag="pgaptmp_003630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000422669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HGU4549495.1"
                     /translation="MELQDWRKEPRKKYSNEFKLRMVELASQPGACMAQIARENGVND
                     DVIFKWLRLWQNEGRISRRLPVTTTSDAGVELLPVEITPDEPKEPMAALTPSLSTQTT
                     VSASSCKVEFRHGNMTLENPSPELLTVKWHTEFGHLNRGDMLTSEQHRCSNEKKKF"
     gene            4013..4127
                     /locus_tag="ACHOSC_RS18155"
                     /old_locus_tag="pgaptmp_003631"
                     /pseudo
     CDS             4013..4127
                     /locus_tag="ACHOSC_RS18155"
                     /old_locus_tag="pgaptmp_003631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477168.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
     gene            complement(4178..5687)
                     /locus_tag="ACHOSC_RS18160"
                     /old_locus_tag="pgaptmp_003632"
                     /pseudo
     CDS             complement(4178..5687)
                     /locus_tag="ACHOSC_RS18160"
                     /old_locus_tag="pgaptmp_003632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001368704.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
     gene            complement(6307..7314)
                     /locus_tag="ACHOSC_RS18165"
                     /old_locus_tag="pgaptmp_003633"
                     /pseudo
     CDS             complement(6307..7314)
                     /locus_tag="ACHOSC_RS18165"
                     /old_locus_tag="pgaptmp_003633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013747467.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing N-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
BASE COUNT         1839 a         2094 c         1870 g         2107 t
ORIGIN      
        1 acaattaatg aattttgatt ggtgacaacc tcaaatgtat tttgaggttg ttatataaaa
       61 cacattattt tatatgattt ggtgatatcc aattagtaga acatgtattt ttcgataaag
      121 taccggtgtt tttttatgaa atttacagag tgcgggaaac agatagccct acgggtcaga
      181 tgtttgatta atattctcat gcactcaata tgctgaataa aaatagatga gtctccttct
      241 ataacatatc tgatgtgact accccattcg tcagtggtca atataccaat gaaaaaaaga
      301 gggaagcagc gccagcaatt cacaacagta taggtattgc tgctgcagcc cgtgagctca
      361 gcctgtatga atcacagtct acaactgggt agtcaacaac aaaatcagta gacgttcttc
      421 tgaacgtgag ctggagatgt ctaccgatgt tgcacgtctc aaacgccagc tggcggaacg
      481 ggatgaagag ctgaccatcc tccaaaaggc cgcgacatac ttcgcgaagc gcctgaaatg
      541 aagtatgtct ttattgaaaa acatcaggct gtgttcaaca tcaaggcaat gtgccatgtg
      601 ctccaggaag gccgcagtga ctggtatatg tggtgtcagc ggcggacaag gataagtaca
      661 tgtcagcagt tccgtcagca ctgcgacagc gtggtactcg cggcttttat ccggtcaaaa
      721 cagcgctacg gagcaccgta ttgacgctta tttattggtg agtactacgt tccatggcag
      781 gagttcgtca acacggttgg agggctattc cggtagtacg ctcagaatat ggcgcagata
      841 cgcttccgga tcgataccgt tcagacggca ggtgccgatc agcccgtaca gcagtgctcc
      901 acgctcgccg ccgtgatcgc taccgaagaa catgtaattc tttttcccga gacagacggc
      961 acgaagcgct ctttccgctg tgttattgtc cgcctccgcc agaccgtcat cactgtaata
     1021 acagagggcg tcccactgat tcagtacata gctgaacgct tcgcctggtc tggatttttt
     1081 cgacagcgtg ccattcttct ccaccatcca ttcatgcagc gacgtcagta acgctttgct
     1141 tcgctgctgc ctgacttcaa gacgctctga ctctggtaat ccccgtattt catcctcgat
     1201 ggcgtacagt tcactgatgc gcttcagggc ttcttctgcc gtcgcacttt tgctgctgat
     1261 gtatacatcg tggatttttc tccggacatg ggctcagcac gcaacttctg tcagcgcacc
     1321 atcttcacgt tctgcactga acagccgatc gtaaccgttg aacgcatccg cctgcagggg
     1381 gcggaggtgt tgctccgggt gtttcccctg ccggttcggt gagtacgcga accagaccgc
     1441 cggaggagat gacgaacccg cattgcgatc attccggaca tacgtccaga tgcgccctgt
     1501 tttcgtcttt tcaggcccag tgccagtacc tttaccggtg tgtcgtcagt gtgaagcttg
     1561 cgggtattca tcacataacg gtacagggca tcattcagcg gagtcattaa ctggcagcac
     1621 gcgtcaaccc agttggagag taatgcacgg ctcagttcga caccctgtcg ggcaaagatt
     1681 tcactctgac gatacagtgg cagctgttcg cagtattttc ccgttaacac gcgggcaagt
     1741 aatccggggc ccgcgatacc acgctctatc gggcgggacg gcgccggtgc ttcaacgatg
     1801 cagtcacatt ttgtacaggc ttttttaccc gaacagtgcg gatcactttc agggcgctgc
     1861 tcaccagttc cagttgttct gcactgactt cctccagata atccagctca ccgccacact
     1921 ccgggcaaca gctttcttca gactccagac ggtgtattgc atggggaagg tgtgccggta
     1981 acggacgacg atggcgcggc tgtctcaact ggcggggaac ctgtggagcg tcttcccgcc
     2041 cactgtaacg atcgctgtcc tgttcgcgtt gtttcagcag ggcctcagcc tgttcaactt
     2101 cacgacgcag tttttcagaa cgggtaccga acagcatccg gcgcagtttt tctatctgag
     2161 tctgcagatg ttctatttcc cgttcatctt cttcgatctt ttcttcggca cgtgccagtg
     2221 cagagcacag gaaggcctcc gtctcttcaa ccagacttag ttgctggtct ttctgacgga
     2281 actggctttc tgaagtggtc aacaaaaact ggccacctag ttagagtttt tccagtatcg
     2341 attttccgat tcgtttgggg gtaacccacc gttatattcg tgcggtctta gtgcgctgta
     2401 atatccaacg atatagtccg ttatggcgtg agctgcctcg ctgaagctta cgtaacccac
     2461 caccggcatc cattcgttct cttcagactc ctgaagaagc gttccattgg gctgttatcc
     2521 cagcagtttc cgcgccggct catactctgt ctgatctggt atcgccacaa taactgccgg
     2581 aactgcctgc tcgtataatg actgccctga tcgctgtgga acatcacccc gccgggctta
     2641 ccacgggttt cccatgccat ttcccgcgct ttcatggtga gcctgctgtc cggcgagaac
     2701 gacatggccc agcccactgg ttttcttgcg aacaggtcga gaacaacggc gaggtacgcc
     2761 cagcgcttac ccgtccagat ataggtcaca tcaccgcacc acacctgatt tggctcggtc
     2821 acggcgaact gcctttcaag gtagttaggg atagcaacat gttcatgacc accacgttta
     2881 taccggtgag tcggctgctg acagctgacc agccccagct ctttcatgag cctgccagca
     2941 agccagcgtc ccatctggta gcctctccgg gttgccattg tggcgatgct tcttgctccg
     3001 gccgaaccgt ggctgatgcc atgtagctca agtacctgac tgcgtaatac agcccgtctg
     3061 ccgtctggtt tttcaggacg gtttttccag tatctgtagc tgctgcgatg aaccccgaac
     3121 acatggcaga gtgtgaccac aggataatgc gctctgagtt tcccgattat cgagaactgt
     3181 tcagggagtc tgacatcaag agcgcggtag ccttttttaa tatttcattc tccatttcaa
     3241 tgcgttgtag ctttttcctc agcttacgta tttcgatttg ttctggtgtt atcggagagg
     3301 cttttggtgt tttgccctga cgctcatcac gcagttgttt gacccatctt gtcattgtag
     3361 aaaggccaac atccatagct ttggcggcat ctgccaccgt gtatttctgg tcaacaacca
     3421 gttgagcgga ttcgcgttta aactctgcgc taaaatttct ttttttcatt agagcacctg
     3481 tgttgttctg aggtgagcat atcacctctg ttcaggtggc caaattcagt gtgccacttc
     3541 acagtgagca gctctggtga aggattttcc agcgtcatgt taccgtgacg gaactccact
     3601 ttgcaggagc tggcactgac agtagtctga gtggataagg atggagtaag agcagccatc
     3661 ggttctttcg gctcatcagg cgttatctct acaggtaata attcaacgcc tgcgtcagaa
     3721 gtggttgtca ccggaagacg ccgtgatata cgcccttcgt tttgccagag tctgagccat
     3781 ttgaaaataa catcatcatt gacgccattt tctcgtgcaa tctgggccat acaagcaccg
     3841 ggttgtgatg ccagttcaac catacgaagt ttgaattcat tcgaatactt tttacgaggt
     3901 tcttttcgcc agtcctgtaa ttccatactt ggatgtccgt ctgtgtcaga tgggcgtcta
     3961 agttaccaat tcttgtctaa tggctacata ctgcggtcgg tttacgctta cttttaacgt
     4021 aaaaaccgtg atggcaagcc tgtgccgtca cggactgagg gcaaaggctt cccggaagtt
     4081 cagcccttac cgcacacagc ctgcctgtgt cagaaaatct gctggagctg tcaacgacgg
     4141 atgaaaagtg cctcttccgc aacctgggtg gagattttca tgccagtgga ctcattaccc
     4201 tctgcctgag tagttcaaat cctgctcgac catacatctg gcgtttcagc atcttcaggc
     4261 gatttacatg accttcgaca acaccattac tccagcgact actgattgcc tcacatatag
     4321 ctgcagcatc agcttccatc cccgccgcca ctcgccgaaa ttctgctgaa ccgctttcgt
     4381 ggactcgagt gaaccaacta cttagctgtg atttattttg ggttttcaga atacggtaga
     4441 actcgagtgc cagttgctgc gctattttca gctcaggttc ttttcacaca tcagactaat
     4501 aaatcgggaa gcataatttt cttcatccct ggttattctc cagggcatca accagcggct
     4561 tacccgggcc accatttccc gccatatccg gctggcgtta taattaccac tttctcgttg
     4621 ctcttttaac cactccctcc atgggtccag aagccctcgc tttggaggtc gggttgacat
     4681 ttcagggaat gttcctgaac gcacccagcg actgactgtc acacgcgaca ggcctgtaat
     4741 acgggatatt tccctgaaac tacatccctt gttatgctgg gccataacct ctgtccaacg
     4801 ctgatgccgt tttttgcggg tttgttgttt taggcgttcc ggacgacgga gcgatgctgc
     4861 aggctcagac agttctggct caggtggttt ctttggtgac aactcgctgg caacaagacg
     4921 tatcagaggt atatgtctgt acatcattcg ttcaagcgca tcgccaatat tttttagcag
     4981 gtggcatcga ccacctgtct ggcctgtggt acaccttcac gtgctgctgt cgcacagacc
     5041 ccactgcgat cacgcgagac aatctgtatt tccggatatt ttctgaacca ggtcgccagc
     5101 gtacgctgat cacggccggg aagcaggacg aggggacggt gagtatcaag attgacgatt
     5161 aacgtaccgt aacggtggcc ccgatgccac gcccactcat caataccgac atgaggcgcg
     5221 cctgactgtt tcgtctccgg ggtattgata accctgcgaa ggagagtatc agcactgcaa
     5281 cggagaccca ctgccgttgc tgcccgtttt ccggcctcac ctccggcgat taatcccagt
     5341 tgacgctgta aattttgtaa cgcctgtgaa gactgcagat gtgaaccacc gaagggaaca
     5401 aacgactcgg caaaaatttt tcgtgaacaa gccgggttac ggctgtacct gtggcggacg
     5461 gaaaatacaa gccatagcgt ctgccctgag cagggtagat gctgtatccg gcgccgacga
     5521 caactatgaa cagagtcgct acgcttaaag cattcaggac attgtgctgt tttgcgtttt
     5581 ccgcggagat ggaggggtat accgtcagag ttaataattt gtcgactaca tcgccatccg
     5641 caaggtaact gtagaagtgt tttaagagag tgcattgcgt tacccataac taaaaacagc
     5701 ttagatcctt gttcaccata aagtttcatc cattttctcc acccaggttg cggaagaacc
     5761 acttttgcac cgttcgtgac aggagcagga tttttacgcc agtagccaga gccagaagtg
     5821 ggcgcgagac atcacgtact tacgtacgga tgaaggctgg ctgtatctgg gagtggttaa
     5881 tgtcgccacg tatgtctgca taagagacct cggaacgtta tgctaccgca tgtcatttgc
     5941 aaaaatgaca gatagatata cttcaattta aaccttaaca attagcatgg gaatgctgtc
     6001 aatcctctgt atgcaaaaat ggcatctgaa tcgctcatta tttagccctc tgtgtcaaag
     6061 cgtaaagtgt tcccagagag cctgtacata gattcgtgta atttcctgat tttgatatgt
     6121 tcatcgacag gaatttatcc cccggaaaga ttaagaaaac aaaaaacgaa catttattca
     6181 acacatccgg aacctcatcg ggtatatatc agcgcttata ttgctgacat tgattgatac
     6241 agggtatcgg tgacgttttt ttgctccatt tccctcaaaa tggacgcaac ttcccctctg
     6301 cggctctcag ccgcaatact gcattcgggc cagcggctca tgcatcataa ccagctctgc
     6361 cagacggtaa ccccgcttca gccccgtaaa ccgcatctga ctttcgcaca ggatgcattt
     6421 cagcgggtca accttcagta accgctgata catccctctc cacctgattt gcatcgccgt
     6481 ttttatcacc gtctctgtta ttacgtacac taattcttcc agcaaccggc gtttcgccgg
     6541 actaaggaac ccgtagaacc tcaccatacg gaacccctta tccgccacat gccaggagaa
     6601 cctttccata aacgcatcac cactcatcaa taggtattct tcacgtcgtg tgcggtgact
     6661 gttgtaacgc agtccgattt catcctgacc ggcatagtgc tccagacggc tcattggcac
     6721 cggcggcttt ttcaggtaag agccaaagta caccgccaca tgtgtggcgt tatccatcac
     6781 ctttgatacg ttgacattcc tgccccgtcg gtaatgactg tccaggaaac gattccattc
     6841 ccgtttactg cttccttcag cagccagctc atccggtatc aatagttccg ggtatttccg
     6901 tgacagtaac cgtgttatcc ggtatcgcta catactcatc accttacggg cgtaaaaatg
     6961 aaggtttttc caggtgtgat gtgatgtcac accgccggca gttgtcgata aatggataaa
     7021 tggatatgtg gatatgtgga tgacactgct ggtcccggcc ccatgtgtgg attaccgtga
     7081 atatccccgg cttcacatct gctgctatgc ggctcatctc tgccagtaac caccggttgt
     7141 ggaacaccag ggaccagtac tggcagggaa gcgtgaatac aattgctgcc acgggccgtc
     7201 ggggaccaga ctcagcagat actgtatcca ctgtgcaccc gctttcaccc cgcagtgtgg
     7261 gcaggagcgt tttttacacc gaaaacagac cttctttgta ttgcaacagt ccgggcagta
     7321 catgccgccg gcgctgatac cggcaagaat ggtcgtaaac tcccgctctg tacagcgggc
     7381 gatttccgga tgcccttcgt catcaacacg tacaaaccag aagaccagct ttttgtttcc
     7441 ggcatccaca aagaacggaa tattcaggtc tgcgcaacac tcaacagtat cgtcaaaatt
     7501 atcgaagcgc agaatctcta cttcttcttc gtcaaaaaaa tcatcttcgt gaagttttac
     7561 aacatagcgg ggaagtttgc ttctttgagg tgcgagtttt cgtttacggg gtttagctga
     7621 acgtgccata taactacctg aaagacaatg acattgtttg tttttataac ggtaatttca
     7681 ggccatgaca agccgcagcc gctaggctgc ctactcgatc gataggaatt taaatcccaa
     7741 aaagattaaa aaaacaccca aaaacggatg tttattcaac actgcctgcg tgccatgagt
     7801 gcaccgccgt caattaaatg gatcttatcg attgatactg catatcactg atggtttttt
     7861 accccattcc cctgaaaatg gacgcaactt ctccgatgcg gctctcagcc
//
LOCUS       DAZKHF020000201         7902 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2201S31241
            SAMN43267800-rid25106413.denovo.203, whole genome shotgun sequence.
ACCESSION   DAZKHF020000201 DAZKHF020000000
VERSION     DAZKHF020000201.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267800
            Sequence Read Archive: SRR30307263
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 7902)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7902)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 7902)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350581.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350581.2
            Genome Coverage       :: 151x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:21:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,652
            CDSs (total)                      :: 5,557
            Genes (coding)                    :: 5,320
            CDSs (with protein)               :: 5,320
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 237
            CDSs (without protein)            :: 237
            Pseudo Genes (ambiguous residues) :: 0 of 237
            Pseudo Genes (frameshifted)       :: 102 of 237
            Pseudo Genes (incomplete)         :: 130 of 237
            Pseudo Genes (internal stop)      :: 52 of 237
            Pseudo Genes (multiple problems)  :: 41 of 237
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7902
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267800-rid25106413.denovo.203"
                     /serotype="O 26:H11"
                     /isolate="2201S31241"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-01"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            300..1571
                     /locus_tag="ACHOSC_RS18170"
                     /old_locus_tag="pgaptmp_003634"
     CDS             300..1571
                     /locus_tag="ACHOSC_RS18170"
                     /old_locus_tag="pgaptmp_003634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic amino acid transport family protein"
                     /protein_id="HGU4549496.1"
                     /translation="MQHNTLSKHNQKLPFTRYDFGWVLLCIGMAIGAGTVLMPVQIGL
                     KGIWVFITAAIIAYPATWVVQDIYLKTLSESDSCNDYTDIISHYLGKNWGIFLGVIYF
                     LMIIHGIFIYSLSVVFDSASYLKTFGLTDADLSQSLLYKVAIFAVLVAIASGGERLLF
                     KISGPMVVVKVGIIVVFGFAMIPHWNFANITAFPQASVFFRDVLLTIPFCFFSAVFIQ
                     VLNPMNIAYRKREADKVLATRLALRTHRISYITLIAVILFFAFSFTFSISHEEAVSAF
                     EQNISALALAAQVIPGHIIHITSTVLNIFAVLTAFFGIYLGFHEAIKGIILNLLSRII
                     DTKKINSRMLTLAICAFIVITLTIWVSFRVSVLVFFQLGSPLYGIVSCLIPFFLIYKV
                     AQLEKLRGFKAWLILLYGILLCLSPLLKLIE"
     gene            complement(1601..2605)
                     /gene="dppF"
                     /locus_tag="ACHOSC_RS18175"
                     /old_locus_tag="pgaptmp_003635"
     CDS             complement(1601..2605)
                     /gene="dppF"
                     /locus_tag="ACHOSC_RS18175"
                     /old_locus_tag="pgaptmp_003635"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide ABC transporter ATP-binding subunit
                     DppF"
                     /protein_id="HGU4549497.1"
                     /translation="MSTQEATSQQPLLQAIDLKKHYPVKKGMFAPERLVKALDGVSFN
                     LERGKTLAVVGESGCGKSTLGRLLTMIETPTGGELYYQGQDLLKHDPQAQKLRRQKIQ
                     IVFQNPYGSLNPRKKVGQILEEPLLINTSFSKEQRREKALSMMAKVGLKTEHYDRYPH
                     MFSGGQRQRIAIARGLMLDPDVVIADEPVSALDVSVRAQVLNLMMDLQQELGLSYVFI
                     SHDLSVVEHIADEVMVMYLGRCVEKGTKDQIFNNPRHPYTQALLSATPRLNPDDRRER
                     IKLTGELPSPLNPPPGCAFNARCRRRFGPCTQLQPQLKDYGGQLVACFAVDQDENPQR
                     "
     gene            complement(2602..3585)
                     /gene="dppD"
                     /locus_tag="ACHOSC_RS18180"
                     /old_locus_tag="pgaptmp_003636"
     CDS             complement(2602..3585)
                     /gene="dppD"
                     /locus_tag="ACHOSC_RS18180"
                     /old_locus_tag="pgaptmp_003636"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0015833 - peptide transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide ABC transporter ATP-binding protein"
                     /protein_id="HGU4549498.1"
                     /translation="MALLNVDKLSVHFGDESAPFRAVDRISYSVKQGEVVGIVGESGS
                     GKSVSSLAIMGLIDYPGRVMAEKLVFNGQDLQRISEKERRNLVGAEVAMIFQDPMTSL
                     NPCYTVGFQIMEAIKVHQGGNKSTRRQRAIDLLNQVGIPDPASRLDIYPHQLSGGMSQ
                     RVMIAMAIACRPKLLIADEPTTALDVTIQAQIIELLLELQQKENMALVLITHDLALVA
                     EAAHKIIVMYAGQVVETGDAHAIFHAPRHPYTQALLRALPEFAQDKERLASLPGVVPG
                     KYDRPNGCLLNPRCPYATDRCRAEEPALNMLADGRQSKCHYPLDDAGRPTL"
     gene            complement(3596..4498)
                     /gene="dppC"
                     /locus_tag="ACHOSC_RS18185"
                     /old_locus_tag="pgaptmp_003637"
     CDS             complement(3596..4498)
                     /gene="dppC"
                     /locus_tag="ACHOSC_RS18185"
                     /old_locus_tag="pgaptmp_003637"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010426221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide ABC transporter permease DppC"
                     /protein_id="HGU4549499.1"
                     /translation="MSQVTENKVISAPVPMTPLQEFWHYFKRNKGAVVGLVYVVIVLF
                     IAIFANWIAPYNPAEQFRDALLAPPAWQEGGSMAHLLGTDDVGRDVLSRLMYGARLSL
                     LVGCLVVVLSLIMGVILGLIAGYFGGLVDNIIMRVVDIMLALPSLLLALVLVAIFGPS
                     IGNAALALTFVALPHYVRLTRAAVLVEVNRDYVTASRVAGAGAMRQMFINIFPNCLAP
                     LIVQASLGFSNAILDMAALGFLGMGAQPPTPEWGTMLSDVLQFAQSAWWVVTFPGLAI
                     LLTVLAFNLMGDGLRDALDPKLKQ"
     gene            complement(4508..5527)
                     /gene="dppB"
                     /locus_tag="ACHOSC_RS18190"
                     /old_locus_tag="pgaptmp_003638"
     CDS             complement(4508..5527)
                     /gene="dppB"
                     /locus_tag="ACHOSC_RS18190"
                     /old_locus_tag="pgaptmp_003638"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005133865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide ABC transporter permease DppB"
                     /protein_id="HGU4549500.1"
                     /translation="MLQFILRRLGLVIPTFIGITLLTFAFVHMIPGDPVMIMAGERGI
                     SPERHAQLLAELGLDKPMWQQYLHYIWGVMHGDLGISMKSRIPVWEEFVPRLQATLEL
                     GVCAMIFATAVGIPVGVLAAVKRGSIFDHTAVGLALTGYSMPIFWWGMMLIMLVSVHW
                     NLTPVSGRVSDMVFLDDSNPLTGFMLIDTAIWGEDGNFIDAVAHMILPAIVLGTIPLA
                     VIVRMTRSSMLEVLGEDYIRTARAKGLTRMRVIIVHALRNAMLPVVTVIGLQVGTLLA
                     GAILTETIFSWPGLGRWLIDALQRRDYPVVQGGVLLVATMIILVNLLVDLLYGVVNPR
                     IRHKK"
     gene            complement(5678..7285)
                     /gene="dppA"
                     /locus_tag="ACHOSC_RS18195"
                     /old_locus_tag="pgaptmp_003639"
     CDS             complement(5678..7285)
                     /gene="dppA"
                     /locus_tag="ACHOSC_RS18195"
                     /old_locus_tag="pgaptmp_003639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide ABC transporter substrate-binding
                     protein DppA"
                     /protein_id="HGU4549501.1"
                     /translation="MRISLKKSGMLKLGLSLVAMTVAASVQAKTLVYCSEGSPEGFNP
                     QLFTSGTTYDASSVPLYNRLVEFKIGTTEVIPGLAEKWEVSEDGKTYTFHLRKGVKWH
                     DNKEFKPTRELNADDVVFSFDRQKNAQNPYHKVSGGSYEYFEGMGLPELISEVKKVDD
                     NTVQFVLTRPEAPFLADLAMDFASILSKEYADAMMKAGTPEKLDLNPIGTGPFQLQQY
                     QKDSRIRYKAFDGYWGTKPQIDTLVFSITPDASVRYAKLQKNECQVMPYPNPADIARM
                     KQDKSINLMEMPGLNVGYLSYNVQKKPLDDVKVRQALTYAVNKDAIIKAVYQGAGVSA
                     KNLIPPTMWGYNDDVQDYTYDPEKAKALLKEAGLEKGFSIDLWAMPVQRPYNPNARRM
                     AEMIQADWAKVGVQAKIVTYEWGEYLKRAKDGEHQTVMMGWTGDNGDPDNFFATLFSC
                     AASEQGSNYSKWCYKPFEDLIQPARATDDHNKRVELYKQAQVVMHDQAPALIIAHSTV
                     FEPVRKEVKGYVVDPLGKHHFENVSIE"
     gene            complement(7637..>7902)
                     /locus_tag="ACHOSC_RS18200"
                     /old_locus_tag="pgaptmp_003640"
     CDS             complement(7637..>7902)
                     /locus_tag="ACHOSC_RS18200"
                     /old_locus_tag="pgaptmp_003640"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4549502.1"
                     /translation="GTALIVGCGATALFDQHTTHFPANFAPHISLSINGFVTETIVFF
                     YICLNLFLRYLWLALAFSAVRFSVHGINRSDIHLFLRFFSLNA"
BASE COUNT         1818 a         2138 c         2024 g         1922 t
ORIGIN      
        1 ttccggcttg ccgctgattt tacgattgct ttttactgtc agcacccggc aatcttcctc
       61 attgcgcgat gccttccagc ttaatttctt tatttgcaat agttgcagtt catatccggg
      121 ctgactccag actggtagtg aaatgcgcaa ttttgtagaa ttgcgcctcc tcgcgacctc
      181 cccagtgaat ctgttgcctc attttttaca tactgcgtat tcgacttctc cacctgttgc
      241 gcaagagaaa ctgggtttat tcatttttgc gagaccgact tctttctgga caggacttta
      301 tgcagcacaa cacactatcg aaacacaatc agaaattgcc gtttacacgc tacgacttcg
      361 gctgggtttt attatgcata ggcatggcga ttggtgccgg aaccgtgctg atgccagtac
      421 aaattggctt gaagggaatt tgggtattta ttaccgcagc gatcattgct tatcctgcca
      481 cctgggtagt gcaggacatt tatttaaaaa ccctttctga aagcgattcc tgtaatgact
      541 acaccgatat tatcagtcat tacctgggga agaactgggg aattttcctc ggggttatct
      601 actttttgat gattatccac gggattttta tctactctct ctccgtggtt ttcgacagcg
      661 cctcgtacct gaaaaccttc ggtttaaccg atgccgatct ttcacaatct ctactttata
      721 aagtcgctat tttcgccgta ctggtggcga ttgcgtctgg tggtgaacga ttactgttta
      781 agatttccgg gccaatggtg gtggtcaaag tagggattat tgtcgtgttc ggttttgcga
      841 tgatcccgca ctggaatttc gccaatataa ccgccttccc gcaagcctcc gtctttttcc
      901 gcgatgtcct gcttacaatt ccattttgct tcttttctgc ggtatttatt caggtactta
      961 acccaatgaa tattgcctat cgtaaacggg aagcagataa agtactggca acccggctcg
     1021 cgctgcgtac ccaccgaatt agttatatca cgctcatcgc ggtgatcctg ttttttgcct
     1081 tttcgtttac cttctcaatt agccacgaag aagccgtttc tgcctttgaa caaaatatct
     1141 cggcactggc gctggccgcg caggtgatcc ctggacatat cattcatatc acctctacgg
     1201 tgctgaatat ctttgccgta ctgaccgcat tctttggcat ttatctcggt ttccacgagg
     1261 ctattaaagg cattattctc aatctgttaa gccgaattat tgataccaag aaaattaact
     1321 cacgcatgct gactctggcg atctgcgctt ttatcgtcat tacattgacg atttgggttt
     1381 cgtttcgtgt atcggtgctg gtgttctttc agttgggaag cccgttatat ggcattgtgt
     1441 cgtgcctcat tccgtttttc ctgatctata aagtcgcaca actggaaaaa cttcgcggat
     1501 ttaaagcctg gctaattctg ctttacggca ttttgctatg cttgtcgcca ctgttgaagc
     1561 tgattgagta aaccggagcg catggccccg gttttgtgag ttaacgctgc ggattttcat
     1621 cctgatcaac agcaaaacaa gcgaccagtt gaccgccgta gtcttttagc tgcggctgca
     1681 actgggtgca ggggccgaag cgccgacgac agcgggcgtt gaaggcgcaa cccggcggag
     1741 gattcagcgg gcttggcagt tcaccggtga gtttgatgcg ttcacgacga tcgtccgggt
     1801 tcaggcgtgg cgtcgcggag agcagcgcct gggtgtacgg atggcgcggg ttattgaaga
     1861 tttggtcctt cgttcctttc tccacgcagc ggcccaggta catcaccatc acttcatcag
     1921 caatgtgctc caccaccgac aggtcgtggg agataaagac ataagaaagc cccaactcct
     1981 gctgcaaatc catcatcaga ttcagcacct gcgcacgcac tgagacgtcc agcgcagaaa
     2041 ccggttcatc ggcaatcacc acgtccgggt cgagcatcag accacgggca atagcgatac
     2101 gctgacgttg tccgccggag aacatatgcg gatagcggtc atagtgctcg gttttcaggc
     2161 cgactttcgc catcatcgac agggcttttt cccgacgctg ttctttgctg aagctggtgt
     2221 taataagcag cggctcttcg agaatttgcc caactttttt acgcggattc agcgaaccgt
     2281 aagggttctg gaagacaatc tggattttct gccgacgcag cttctgcgcc tgcggatcgt
     2341 gcttgagcag atcctgcccc tggtaataca gctcaccacc ggtgggcgtt tcgatcatcg
     2401 tcagcaaccg accgagggtc gatttaccgc agccagattc gcccactact gccagcgttt
     2461 tgccacgttc aaggttaaac gaaacgccgt ccagcgcctt caccagacgt tccggcgcga
     2521 acatgccttt cttcaccgga taatgttttt tcaggtcgat agcctgcaac agcggttgtt
     2581 gcgaggtggc ctcttgcgta ctcatagtgt cggcctcccg gcatcatcaa gtgggtaatg
     2641 gcatttggac tgacgcccat cagcgagcat attcagcgcc ggttcttcag cgcgacatct
     2701 gtcagtggca taggggcagc gcgggttaag caggcagccg ttcgggcggt cgtacttacc
     2761 gggaacgaca cctggcaacg acgccagacg ttctttgtcc tgagcaaatt ctggcagcgc
     2821 acgcagcaat gcctgagtat acgggtgacg cggcgcatgg aagatggcgt gcgcatcacc
     2881 ggtttccacc acctggcctg catacatcac gatgatttta tgtgccgctt ccgccaccag
     2941 cgccaggtca tgggtaatta acaccagcgc catgttctct ttctgctgta gctccagcag
     3001 tagttcgatg atttgcgcct gaatggtcac gtccagcgcg gtggtcggtt catcggcaat
     3061 cagcagtttt ggccgacagg caatcgccat ggcgatcatc acgcgctggc tcatgccgcc
     3121 ggaaagctga tgcgggtaaa tatccagacg cgatgccgga tcgggaatac cgacctgatt
     3181 cagcaggtca atcgcccgct gacggcgagt gctcttatta ccgccctgat gcaccttaat
     3241 cgcttccatg atctggaaac ccacggtgta gcacgggtta aggctggtca tcgggtcctg
     3301 gaagatcatc gccacttcgg cacccaccag gttgcggcgc tctttttctg atatacgctg
     3361 caaatcctga ccgttaaaca ccagcttttc cgccattacg cggcccggat aatcaatcag
     3421 ccccataatc gccagtgaac tgaccgactt accggaacca gactcaccca caatcccgac
     3481 cacttcgccc tgttttacgc tgtagctgat gcggtctacg gcgcggaacg gtgcgctttc
     3541 gtcgccgaaa tgcaccgata atttatctac atttaataac gccatctcga acctcttact
     3601 gcttcagttt gggatcgagc gcgtcacgca gaccgtcacc catcaggtta aatgccagca
     3661 ccgtcagcag gatcgccaga cccgggaagg tcacgaccca ccaggcgctt tgcgcgaact
     3721 gcaacacgtc ggagagcatg gtgccccact ctggtgttgg cggctgcgct cccatgccga
     3781 ggaagccaag ggcggccata tcgagaatgg cgttagagaa accgagcgac gcctgaacaa
     3841 tcagcggcgc aaggcagttc gggaagatgt taataaacat ctgacgcatc gccccggcac
     3901 ccgccacgcg agacgcggtg acgtaatcgc ggttaacttc caccagcacg gcggcgcggg
     3961 ttaagcgcac atagtgcggc aaggcaacga aggtcagtgc cagcgcggcg ttaccaatcg
     4021 acgggccgaa gattgccacc agcaccagcg ccagcagcag acttggcagc gccagcatga
     4081 tatcgaccac gcgcataatg atgttatcga ccaggccgcc aaagtaaccg gcgatcaggc
     4141 cgagaataac gcccataatc agcgataaca caactaccag acagccaacc agcagcgaca
     4201 ggcgcgcacc gtacatcagg cgcgacagca catcacggcc tacgtcatcg gtgcccagca
     4261 agtgcgccat gctgccgcct tcctgccagg ctggcggggc gagcagtgca tcgcggaact
     4321 gttccgccgg gttatagggt gcaatccagt tggcaaagat cgcgatgaac agcacgatga
     4381 cgacgtaaac cagcccgacg accgcgcctt tgttgcgttt aaaatagtgc cagaactcct
     4441 gtaacggggt catcggcacc ggtgcgctaa tcactttatt ttcagtaacc tgtgacatga
     4501 tgtcccctta cttcttatga cgaatacgcg ggttcaccac gccgtacagc agatcgacca
     4561 gcaagttgac gaggataatc atcgtcgcca ccagcaatac gccgccctgc actaccggat
     4621 agtcgcggcg ttgcagcgcg tcaatcaacc agcgccccag accgggccac gagaagatgg
     4681 tttcggtcag aatcgccccc gccagcaatg ttcccacctg caggccgata acggtcacta
     4741 ccggcagcat cgcgttacgc agcgcatgga cgataatcac ccgcatgcgg gttagccctt
     4801 tggcgcgcgc ggtgcggatg taatcctcgc ccagcacttc cagcatcgag gagcgtgtca
     4861 tacgcacaat gaccgccagc ggaatagtac ccagcacaat ggcaggcaag atcatatggg
     4921 cgacggcatc gataaagttg ccgtcttcac cccagatggc ggtgtcgatt agcataaaac
     4981 cggttaacgg attggagtca tcgaggaaca ccatatcgct cacgcgaccg gagacgggcg
     5041 tcaggttcca gtgcaccgaa accagcatga tcagcatcat gccccaccag aagatcggca
     5101 tcgagtagcc ggtcagcgcc aggccaaccg ctgtgtgatc gaaaatggaa ccgcgtttaa
     5161 ccgcagccag cacgccgacc ggaataccga ccgcagtcgc aaaaatcatc gcgcagacgc
     5221 caagttccag cgtggcctgg aggcgcggca cgaactcttc ccaaaccggg atgcggcttt
     5281 tcattgaaat gcctagatcg ccatgcataa cgccccaaat gtaatggaga tactgctgcc
     5341 acatcggttt atctaagccg agttcagcca gcagctgcgc gtgacgctct ggggagatcc
     5401 cacgttcgcc cgccatgatc atcaccggat cgcccgggat catgtggaca aaggcaaatg
     5461 tgagaagggt aataccgata aacgtgggga tgacgagtcc caaacgtcgg agaataaact
     5521 gcaacataac ccggattctc tatagtgacc cgcagcctgg aactgcgtgt ctgtattgct
     5581 cacaaatcaa ttcccgccta ggtcgggctg ggaatgaagt attgctgccg gatggcgctg
     5641 cacgcagagg cacagcgcca ccgggtatgg cttttaatta ttcgatagag acgttttcga
     5701 agtgatgttt gcctaatgga tcaaccacat agcctttaac ttctttgcgt accggttcaa
     5761 acacggtgga gtgagcgatg atcagtgccg gagcctgatc gtgcatcacc acctgcgctt
     5821 gcttgtacag ttcaacgcgt ttgttatggt cgtcggtagc acgcgccggt tgaatcagat
     5881 cttcaaacgg tttgtagcac cattttgagt agttggagcc ttgttcagag gcggcgcagc
     5941 tgaacagggt ggcgaagaag ttatccggat ccccgttatc gccagtccag cccatcatta
     6001 ccgtctggtg ctcgccatct ttcgcacgct tgaggtactc accccattcg taggtgacaa
     6061 ttttggcctg cacgccgact ttcgcccagt cagcctgaat catctccgcc atgcggcgag
     6121 cgttcgggtt atatgggcgc tgtaccggca tcgcccacag gtcgatggag aaaccttttt
     6181 ccagacccgc ttctttcagc aaggctttcg ctttttcagg atcgtaggtg taatcctgaa
     6241 cgtcgtcgtt atagccccac atggttggcg ggatcaggtt tttcgctgat acacccgcgc
     6301 cctgataaac cgctttgatg atcgcgtctt tgttcaccgc gtaggtcagt gcctggcgaa
     6361 ctttcacgtc atccagcggt tttttctgca cgttatagga gaggtagccg acgttcagcc
     6421 ccggcatttc catcaggttg atggatttat cctgcttcat gcgagcgatg tctgccgggt
     6481 tcgggtacgg catcacctgg cattcattct tctgcaattt cgcataacgc acggaagcgt
     6541 caggagtaat agagaagacc agcgtatcga tctgcggttt ggtgccccag tagccatcaa
     6601 acgctttgta gcggatacgg gagtcttttt ggtactgctg taactggaac ggaccggtgc
     6661 cgattgggtt gaggtccagt ttttccggtg taccggcttt catcatcgcg tcagcatatt
     6721 cttttgacag aatagaggcg aagtccattg ccaggtcagc gaggaacggc gcttccgggc
     6781 gagtcagcac aaactgaacg gtgttgtcgt ccaccttttt cacttcgctg atcagctccg
     6841 gcaagcccat gccttcgaag tattcgtagc tgccgccaga aactttatgg tacgggtttt
     6901 gcgcgttttt ctgacgatca aacgagaaca ccacgtcatc ggcgttcagt tcacgcgtcg
     6961 gtttgaattc tttattgtcg tgccacttca cacctttacg cagatggaag gtataggttt
     7021 taccgtcttc gctgacttcc cacttttcag cgaggcccgg gatcacttca gtggtgccga
     7081 ttttaaattc aaccaggcgg ttataaagcg gtacggaaga ggcgtcatag gtggtgccgg
     7141 aggtaaacag ctgcgggtta aacccttccg gagatccttc tgagcaataa accagagttt
     7201 tagcctgaac acttgctgcg acggtcatag ccaccaggct gagaccaagc ttcagcatcc
     7261 ctgacttttt caaggaaata cgcattattc tgctccaatt gtgatgtttg ttgttttaac
     7321 cctttgcagt gggttgtcgc tgcctgacct tttttgtttt ttgcccggtc gggtcaacgt
     7381 tatgaggtgg ggatgccgta ctaattaaca tcagtgtaac agtgcggatc ctccataaaa
     7441 tgcccctcgt gacctacaat ctgtcaacag aatgtgaaaa cgtcaataca gccaaccggg
     7501 atttacacca acggtgagaa tccacaaaca aagattaaaa aaacttcaaa cagctatttg
     7561 cagcagagaa atttgtgcta ctccaacatg accagaacaa tcagcttaat atttagcaac
     7621 atttggtgat taagttttat gcatttaatg aaaaaaatct gaggaaaagg tggatatctg
     7681 agcgattaat accatgaacg ctaaaacgca cagcggaaaa tgccagcgcc agccataagt
     7741 aacgcaaaaa aagatttaag caaatataaa aaaagacaat ggtttctgtc acaaatccgt
     7801 taatggatag tgagatatgg ggcgcaaagt tggcaggaaa atgtgtggtg tgttggtcga
     7861 ataaggcggt cgcgccgcat ccgacaataa gtgcggtgcc gt
//
LOCUS       DAZKHF020000202         7902 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2201S31241
            SAMN43267800-rid25106413.denovo.204, whole genome shotgun sequence.
ACCESSION   DAZKHF020000202 DAZKHF020000000
VERSION     DAZKHF020000202.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267800
            Sequence Read Archive: SRR30307263
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 7902)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7902)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 7902)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350581.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350581.2
            Genome Coverage       :: 151x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:21:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,652
            CDSs (total)                      :: 5,557
            Genes (coding)                    :: 5,320
            CDSs (with protein)               :: 5,320
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 237
            CDSs (without protein)            :: 237
            Pseudo Genes (ambiguous residues) :: 0 of 237
            Pseudo Genes (frameshifted)       :: 102 of 237
            Pseudo Genes (incomplete)         :: 130 of 237
            Pseudo Genes (internal stop)      :: 52 of 237
            Pseudo Genes (multiple problems)  :: 41 of 237
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7902
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267800-rid25106413.denovo.204"
                     /serotype="O 26:H11"
                     /isolate="2201S31241"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-01"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            complement(<1..563)
                     /locus_tag="ACHOSC_RS18205"
                     /old_locus_tag="pgaptmp_003641"
     CDS             complement(<1..563)
                     /locus_tag="ACHOSC_RS18205"
                     /old_locus_tag="pgaptmp_003641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001317900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SEC-C metal-binding domain-containing protein"
                     /protein_id="HGU4549503.1"
                     /translation="MGNNKVKLGRNDKCWCNSGLKYKKCHLGRDRETPVSKAEALKFS
                     KSLSQRKACYAPASMHEDCDKRIVQAHTISKSSSLKAIADSSNHVMGVIMNLASLMRN
                     NGKWLPEKIGINQASTFTGFCAVHDRILFSCLENEKFTGTDEQCFALMFRSLSKEIYA
                     KEGGVLSSDFAKNADKGKLQVEQYYIQ"
     gene            complement(963..2063)
                     /locus_tag="ACHOSC_RS18210"
                     /old_locus_tag="pgaptmp_003642"
     CDS             complement(963..2063)
                     /locus_tag="ACHOSC_RS18210"
                     /old_locus_tag="pgaptmp_003642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001494339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage late control D family protein"
                     /protein_id="HGU4549504.1"
                     /translation="MTDTTMQLLSQSTDPVKMPDFDILAEGETLSGVAERLMSLSLTD
                     NRGFEADLLTITLDDADGQLQLPPRGARLTVLIGWKGEPLTEKGTYIVDEIAHEGPPD
                     RLTVSARSADFRDEFNVKREVSWHDVTVERVVSAIAHRYGLKPQISEMLMDIEIDHAD
                     QTEESDMSFLTRMAEMLGAITTVKSSNLLFIMPGGGVNAQGQPLPSFAITRSSGDRHQ
                     FRIADREAYTGVRAYWLDLNYGKKKKVSVKRRKPKKEKSSSREGDYMEGAEGNVFVLR
                     KTYQNEQAARRAAAAKWQQLQRGAASFSITLARGRAELYPEMHGTVTGFKSDIDNQDW
                     IIAKAEHTIDNSGFTTQLELEAKIPEWIAETE"
     gene            2221..3405
                     /locus_tag="ACHOSC_RS18215"
                     /old_locus_tag="pgaptmp_003643"
     CDS             2221..3405
                     /locus_tag="ACHOSC_RS18215"
                     /old_locus_tag="pgaptmp_003643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail sheath subtilisin-like
                     domain-containing protein"
                     /protein_id="HGU4549505.1"
                     /translation="MSETRFHGARVTESTDLVTAINDVDSSVIGIVATADDADAELFP
                     LNKPTLLTRVNDVLGKCGTTGTLYRALKAIADQVSTKVIVVRVAEHKEEGGKTQDQLV
                     IGGSESDGSYTGMYALLVAEQDESIGYRPRILAAPELDTEAVTKSLCVIAGKLRAFVY
                     ASCHDCNTMAEAITYRQKFNEREVMLLWPDFIAYNPKSGKNETFPAPAYACGLRAYID
                     HEQGWHKSLSNVPVKNVLGMSRHVFWSLQAEDSDANSLNNKEITTIIRRNGFRFWGNR
                     TPETNAYIFEVYTRTAQVLADSIAEAQFETIDSPLTPANVKDVISAIRAKLDSLVTAG
                     KLIGAECWYDVVDNSTTDLRQGRVRIRYKYTPVPPLEDMELYQTFTDEYFEPAFAVLG
                     GA"
     gene            3405..3917
                     /locus_tag="ACHOSC_RS18220"
                     /old_locus_tag="pgaptmp_003644"
     CDS             3405..3917
                     /locus_tag="ACHOSC_RS18220"
                     /old_locus_tag="pgaptmp_003644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016032 - viral process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage major tail tube protein"
                     /protein_id="HGU4549506.1"
                     /translation="MAVPKHLRFFTLFVDGENEVGKVTSVTLPKLTRKTDSYRGGGMM
                     GAVSIDLGLDDSALDASFVMGGAVRELFLKYGGTIDGTLLRFAGEYYTDAESDLYEVE
                     MRGRVTEIDMGEAKQGEATSHTYAIKNTYYKLSVNDRPLWEIDLLNFIYRKDGKDIVP
                     DRIRSALGLG"
     gene            3972..4337
                     /locus_tag="ACHOSC_RS18225"
                     /old_locus_tag="pgaptmp_003645"
     CDS             3972..4337
                     /locus_tag="ACHOSC_RS18225"
                     /old_locus_tag="pgaptmp_003645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HGU4549507.1"
                     /translation="MKETKNIDTENTVVADTVKETSERGVKLTQPIERGGEKITYVEI
                     TGAIEQAGSLRDLSLSDVLNLKAESMFTLLSRVTSPRLDEVTIKKMASRDFIQLCVVA
                     VNFLSGADSGGKNEQATEA"
     gene            4346..4501
                     /locus_tag="ACHOSC_RS18230"
                     /old_locus_tag="pgaptmp_003646"
     CDS             4346..4501
                     /locus_tag="ACHOSC_RS18230"
                     /old_locus_tag="pgaptmp_003646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000333494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GpE family phage tail protein"
                     /protein_id="HGU4549508.1"
                     /translation="MCFEHIEDFVADIAVIFNWSPAEIFMMTPGEVVSWRERAALRSG
                     NADNEDS"
     gene            4488..7295
                     /locus_tag="ACHOSC_RS18235"
                     /old_locus_tag="pgaptmp_003647"
     CDS             4488..7295
                     /locus_tag="ACHOSC_RS18235"
                     /old_locus_tag="pgaptmp_003647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000853438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016032 - viral process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tape measure protein"
                     /protein_id="HGU4549509.1"
                     /translation="MKTLDIRVAFSAVDRLTRPAENARRLMGQFGDSIQRTQGAIKNL
                     ERQARSFERARDAVSKADAGIVKARRQLNALNQLQRTGTVLSEKQQKLMQQLSTRLER
                     LNESRTREIQKMRELGGELKRHGISLTGSDNTIQQAIRRTEQYNNQLERERQALARVT
                     RARKWYSRAQETAGKLKTGGALAIGAAAAGGYAAGRFLQPAIGFGKEMSRVQALTRID
                     QNSPQFKALREQALKLGSETQFTAGDAASGQAFLAMAGFTPQAIQAALPGVLSMATAG
                     GMDLGETADIGSNILTQFGLSADQMDRVGDTLTAAFTRTNTDLRALGETMKYAGPVAG
                     KLGISLEQAAAMAGVLANMGIRGSDAGTAMRASLARLASPPKAAAEALKELGVSVSDA
                     GGKMRPMEDVLADLYKATRKYGEVDRVSFFKDIAGEEAFTSFMALVDAVGDGSLPKLR
                     KELEGARGEAERTAKVMANNLDGDLKSLGSAWEGLRIRIADLIDGPLRSVTQWLTRVV
                     SRVTALAQAHPALTRQLLIAGGALLAMTATVGSLSLAIGVLAGPLAKLRLGFSLLTGS
                     MNAVRVLPALWGMVTGSVSLLGGAIGALFSPVGLIVAALAGAAVLIWKYWDPIRAFFA
                     GVFSGIMERLNPLRETFERFGPVFDAIGSGISQVFNWFKSLLSPMESSKETLDKCTSA
                     GEIFGNVLGGALQLVLTPAKMLLDTLAWILEKLGVLPDEAERARKKIEDAQRAAILQD
                     KVALLQGDLAKINPPKPVENGNGTGGDKPKDNKPLTDSNTGTLRRLSKIADNTGKLVD
                     ETKKRIGPGDIVFKNLPRALAVRGEWQERKIAQVSKPAPAINITPVVPAPLPPALVPV
                     VAASSRPVAEAIRSPVASVPAASRNREPAVSRFGGEIHVHLHNVVTQNPRELAKLVGE
                     MVRAEMERRARAGRGSFYDKD"
     gene            7308..7796
                     /locus_tag="ACHOSC_RS18240"
                     /old_locus_tag="pgaptmp_003648"
     CDS             7308..7796
                     /locus_tag="ACHOSC_RS18240"
                     /old_locus_tag="pgaptmp_003648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310666.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HGU4549510.1"
                     /translation="MMMIYGMFVFELRTLPHQQLQQNKSWRHVKNERVNRSASWQYIG
                     AGDDRIVLSGVLYPEITGGEVSLSLLTTQAYTGRPWPLIDGVGQIYGMYVLTETNTTR
                     SEFDRYGKSKKIEFSLTLERCDEDLRERLQSSSFSDMLSGFKDKVTSSLNSATSSVKG
                     LL"
BASE COUNT         1817 a         1960 c         2220 g         1905 t
ORIGIN      
        1 ttctgaatat aatactgttc aacttgtagc tttcctttat cggcattttt agcaaaatca
       61 gaagatagca ctcccccttc tttggcataa atttctttag acaatgaacg aaacattaag
      121 gcaaagcact gctcgtccgt gcctgtaaat ttttcgttct ctaggcatga aaataagatt
      181 ctgtcatgaa ctgcacaaaa tcctgtaaaa gtggatgcct gattgatacc gatcttctcg
      241 ggaagccatt ttccgttgtt tctcatcagg cttgctaagt tcattatcac ccccatgacg
      301 tgattgctgg agtcggcgat cgccttaagg ctactgctct tagatatcgt atgcgcctga
      361 acgatgcgtt tatcacaatc ttcgtgcatg gatgctggcg cataacatgc ctttctctgc
      421 gagagagact ttgaaaattt cagtgcctcg gctttggata ccggagtttc acgatctcga
      481 ccaagatggc actttttata cttaagtcct gaattacacc agcacttatc atttcttcct
      541 aatttcactt tattgtttcc cacgaaatta atgttctata acaaaaacac ttatcgaaaa
      601 taggtgtaat ttatcactcc ggcagctaaa agatcatctg ccacatgcac gataataaat
      661 cttccttgcg tcatcatctc cctctgttct taaatcaggt gttgcctgtg tgcggaggat
      721 tcgcgaagta cagtagccga aatgcatgat cttacccatc aatggtgagc acgcgataat
      781 aagtgagtcc ctaattgacc actcatggca atacaacttc aaccagcatt catgaccact
      841 atccgtggat taactgttac ataagcaaca cctgctctag gcacaaagcg ggctgccaga
      901 ttgggtctgg cactgtgctg tgagtgtgtc agttctggtt tgagtcaata catctaattt
      961 gctcattctg tttctgctat ccattccggg atttttgctt caagctcaag ctgtgtggta
     1021 aagccgctgt tatcaatggt gtgctcggct tttgcaataa tccagtcctg attatcgatg
     1081 tcacttttga agcctgtcac cgtgccatgc atttcgggat agagttctgc gcgtccacgc
     1141 gccagcgtga tggagaatga tgcggctccg cgttgtagct gctgccactt tgccgccgct
     1201 gcacgtcttg ctgcctgctc gttctgataa gtcttgcgta acacaaacac gttgccttcc
     1261 gcaccttcca tatagtcacc ttcacggctg ctgcttttct cctttttcgg tttgcggcgt
     1321 ttcacgctga cttttttctt tttcccgtaa ttaagatcaa gccagtaagc acgtaccccc
     1381 gtatacgctt cgcggtcagc aatgcggaac tgatggcgat cgccactgct gcgcgtgatg
     1441 gcgaacgatg gcaacggctg gccctgtgcg ttcacgccac cgcctggcat gatgaataac
     1501 agattactgc tttttaccgt agtgattgcg cccagcattt ccgccatgcg cgtaaggaag
     1561 gacatgtcgc tttcttcggt ctggtcggcg tggtcgattt caatatccat cagcatttcg
     1621 ctgatttgtg gtttcagacc gtaccgatga gcgatggcgg ataccacgcg ctcaacggtc
     1681 acatcatgcc aggacacctc acgtttaacg ttaaattcat cccgaaaatc tgcgcttctg
     1741 gctgaaacag tcagcctgtc cggcggtcct tcgtgagcga tttcatcaac aatgtaagtg
     1801 cctttttctg tcagcggttc tcctttccag ccaatgagaa ccgtcaggcg cgcgccccgt
     1861 ggcggtagct gcaactgacc atccgcatca tccagcgtga tggtgagcag gtccgcttca
     1921 aatccccggt tgtcggtcag tgacaggctc atcaggcgct ctgccacgcc ggacagtgtt
     1981 tccccctccg cgagaatatc aaaatccggc attttcacgg ggtctgtgct ctgactgagc
     2041 aattgcatgg tggtatcggt catctgttcc ctccctgtgc ggcatggtcg catgtgcgtg
     2101 catagggggt tactgctttt tgttgtcgcc gtggcgggag aacggcgcag gggtgagatt
     2161 acgcgcgtgg tgggtgatga ttgttgccga atcatttaac ggatacaagg ggctgaagct
     2221 atgagtgaaa ctcgttttca tggtgcccgt gttacggaaa gtaccgacct ggtaacagcg
     2281 attaatgatg ttgattccag tgttatcggt atcgtggcaa cggcggatga tgcggacgcg
     2341 gagctgttcc cgctgaacaa gcccacactg ctgacccgcg tcaatgacgt gctgggaaaa
     2401 tgcgggacta cggggacgct ttatcgtgcg cttaaggcta ttgcagacca ggtgagcaca
     2461 aaagtgatcg tcgttcgcgt ggctgaacac aaagaagaag gcggtaagac gcaggatcaa
     2521 ctggttatcg gtggttctga atctgacggc agctatacgg ggatgtatgc gctgcttgtt
     2581 gcagagcagg atgaaagcat cggataccgt ccgcgtattc tggccgcgcc ggagctggac
     2641 acggaggcgg tgacaaaatc cctgtgcgtg attgcgggta aactgcgcgc gtttgtgtat
     2701 gcctcatgtc atgactgtaa cacgatggct gaggcaatta cctaccgcca gaaattcaac
     2761 gaacgtgagg tgatgctctt atggccggac ttcatcgcct acaacccgaa aagtggcaaa
     2821 aacgaaacgt tccccgcgcc tgcctatgcg tgcggccttc gtgcgtacat tgaccatgag
     2881 cagggctggc acaaatcgct gtccaacgtt cctgttaaaa atgtgctggg gatgtccagg
     2941 catgttttct ggtcgttgca ggccgaagac agtgatgcca acagcctcaa caacaaagaa
     3001 atcacgacca ttattcgtcg caacgggttc cgcttctggg gcaaccgcac accggaaacg
     3061 aacgcctaca tctttgaggt gtatacccga accgcacagg tgctggctga ttcaattgcg
     3121 gaagcgcagt ttgaaaccat cgacagtcca ctgacgcctg cgaacgtgaa ggatgttatc
     3181 agtgccatca gggcaaaact ggattcgctg gttactgccg ggaaactgat tggcgcggag
     3241 tgctggtatg acgtggtgga taacagcacc acggatttac gtcaggggcg tgtgcgtatt
     3301 cgctacaaat atacgcccgt tccgccactg gaagacatgg agctttacca gacgtttact
     3361 gatgaatact ttgaacccgc atttgcggtg ctgggaggtg cctgatggct gtaccaaaac
     3421 atcttcgctt ttttacgctg tttgtggatg gtgaaaacga agtgggtaag gtgacgtccg
     3481 tcactctgcc taagctgacg cgcaaaaccg acagctaccg gggtggtggc atgatgggtg
     3541 cggtaagtat tgatctcggt ctggacgact ccgcgcttga tgcgagcttt gtcatggggg
     3601 gcgcagttcg tgagctgttc cttaagtatg gcggcacgat tgacggcacg ctgctgcgtt
     3661 ttgcgggtga atattacacc gatgcagaaa gcgacctgta tgaagtcgaa atgcgcggac
     3721 gtgtgacgga aattgatatg ggggaagcca aacagggcga agccacatca cacacttacg
     3781 ccattaaaaa cacctactac aagctgagtg ttaacgatcg cccgttgtgg gagattgacc
     3841 tgctgaactt catttaccgg aaggacggca aggacattgt gcccgatcgc atccgttccg
     3901 cgctcgggct tggctgataa gtaatatgca ggcggcgcag tgcgtcgcct ctgactgaaa
     3961 ggagtttcct gatgaaagag acgaaaaaca tcgataccga aaacacggta gttgctgaca
     4021 ctgtgaaaga aaccagtgag cgtggtgtaa aacttaccca accaattgag cgaggcggcg
     4081 aaaaaatcac gtatgtggag atcaccgggg ctattgagca ggctggatct ctgcgagatt
     4141 tgtcgctgtc tgatgtgctg aatctgaaag cggaatccat gtttacgctg ctgtcacgcg
     4201 tgacatcacc gcgactggat gaagtgacga tcaaaaaaat ggcatcccgt gactttattc
     4261 agttatgtgt ggttgccgta aattttttga gcggtgcgga ctctggcggg aagaacgaac
     4321 aggcgacgga agcctgatca cggttgtgtg ctttgagcac atagaagact ttgtggcaga
     4381 tattgccgtt atttttaact ggtcgcccgc cgaaatcttc atgatgacgc ccggcgaagt
     4441 ggttagctgg cgtgagcggg cggcacttcg cagcgggaat gcagacaatg aagactcttg
     4501 atatccgggt cgctttcagc gccgttgaca ggctgacccg gcctgccgaa aacgcccgcc
     4561 gcctgatggg gcagtttggt gactccatcc agcgaacgca gggggcgatt aaaaatctcg
     4621 agcgtcaggc tcgttcattt gagcgcgccc gcgacgctgt cagtaaagcg gatgcgggta
     4681 tcgtgaaagc acgacgccag cttaacgccc ttaatcagtt acaacgcacg ggtacagtgc
     4741 tcagcgaaaa acaacaaaag ctgatgcagc agttaagcac ccggcttgaa cgcctgaatg
     4801 aatcgcgcac acgggaaatt cagaaaatgc gggagcttgg cggagagctg aaacgccacg
     4861 gcatttccct gacaggcagc gataacacca tccagcaggc catcagacgc accgaacagt
     4921 acaacaacca gcttgaacgc gaacggcagg cgcttgcgcg tgtaacgcgg gcgcgtaagt
     4981 ggtattcgcg cgcgcaggaa accgcgggaa aactgaaaac aggtggtgcg ctggcaattg
     5041 gtgcggcagc ggcgggcggc tatgctgccg ggcgtttttt gcagcccgcg atcgggttcg
     5101 gcaaagagat gtcccgcgtt caggcgctga cgcgaatcga ccagaacagc ccgcagttta
     5161 aggcgctgcg tgagcaggcg ttaaaacttg gctctgaaac gcagttcacc gcaggcgatg
     5221 ccgccagtgg gcaggcattt cttgcaatgg caggcttcac accgcaggcc attcaggctg
     5281 cgcttcccgg agtgctgagc atggcaacgg ctggcggtat ggatctcggc gagacggcag
     5341 atattggctc aaatatcctg acgcagttcg gcctttctgc tgaccagatg gaccgggtcg
     5401 gtgacacact caccgcagcg tttacccgta ccaacactga ccttcgcgca ctgggcgaaa
     5461 ccatgaaata tgcaggtccg gtggcgggta agctgggaat atcgctggag caggccgcag
     5521 cgatggctgg tgtgctggcg aatatgggca tcagagggag tgatgccggg acggcaatgc
     5581 gtgccagcct ggctcgtctg gcatcaccgc caaaggcggc ggcagaagct ctgaaagagc
     5641 tgggcgtgtc cgtctcggat gccgggggta aaatgcgccc gatggaggat gtgctggccg
     5701 acctttataa agccacccgc aaatacgggg aagttgaccg ggtatcgttc tttaaggaca
     5761 ttgccggaga agaggctttc acatcgttta tggcgctcgt tgatgcagtg ggtgacggtt
     5821 ccttacccaa actgagaaaa gaacttgaag gcgcgcgcgg tgaggctgaa cgcacagcaa
     5881 aggttatggc caacaacctt gacggcgatc tgaaatcact cggcagtgca tgggaagggt
     5941 tgcgcatccg cattgcagat ctgattgacg gtccgctgcg ttctgtcacg cagtggctca
     6001 cgcgggtggt atcaagggtg acggcgctgg cgcaggccca tccggcactg acgcgccagc
     6061 tactgatagc aggcggtgcg ttgctggcaa tgactgcaac ggttggctcg ttgtcgctgg
     6121 ctattggtgt gcttgctggt ccgctggcaa aactgcgtct tggtttttct ctcctgaccg
     6181 gatcaatgaa tgctgtcagg gtcctgccag cactatgggg aatggtgacg ggttccgttt
     6241 ctttactggg aggcgctatc ggggcgctgt tcagtccggt tggtcttatc gtggctgcgc
     6301 ttgccggagc tgccgtcctt atctggaaat actgggatcc catcagggca ttttttgccg
     6361 gggtgttcag cgggattatg gaaaggctga acccgttgcg tgaaaccttt gaacggtttg
     6421 gccctgtttt tgacgcaatc ggaagcggga tcagccaggt gtttaactgg tttaaatcgc
     6481 tgctgtcacc gatggagtcc agcaaggaaa cgttggataa atgtaccagt gctggcgaga
     6541 tattcggtaa cgttcttggc ggtgcgttac aacttgttct gacacctgca aaaatgctac
     6601 tggatacgct ggcgtggata cttgaaaaac ttggcgtcct tccggatgaa gcggaaaggg
     6661 cgcgcaagaa aatcgaagac gcacagcgtg cggccattct tcaggataag gttgccttac
     6721 ttcaggggga cctggcgaaa atcaatccgc cgaagcctgt ggaaaatggc aatggcaccg
     6781 gaggtgataa acccaaagac aacaaaccgc tcacagacag caataccggg acgctacgca
     6841 gactcagcaa aattgctgat aacacaggta agctggttga tgagacgaaa aaacgcattg
     6901 gccctggcga tattgtcttt aagaacctgc cccgcgcact tgctgttcgt ggggagtggc
     6961 aggagcggaa gattgcgcag gtcagtaagc ctgcccccgc aattaatatc acacccgtag
     7021 tcccggctcc gctgcctccg gcgctggtcc ctgttgttgc ggccagctcc cgcccggtgg
     7081 cggaggccat acgatcgcca gtggcatcag ttcctgcagc ttcccgtaat cgggagcctg
     7141 ctgtctccag atttggcggt gaaattcatg ttcatctgca taacgttgtt acgcagaatc
     7201 cccgcgaact ggcgaaactg gtcggtgaaa tggtcagggc agaaatggaa cggcgcgccc
     7261 gtgccgggcg tggcagtttt tacgataaag attgaggagt catggccatg atgatgatct
     7321 atggcatgtt tgtttttgag ctgcgcacgc tgccgcatca gcagttacag caaaacaaaa
     7381 gctggcggca tgtgaaaaat gaacgcgtta accgttcagc aagctggcag tatatcggtg
     7441 caggtgatga tcgcatcgtt ctttctggtg tgctttatcc tgaaattaca ggtggcgaag
     7501 tgtcgctgtc tctgctgacc acgcaggcgt atacaggacg gccctggcct ctgattgatg
     7561 gcgtcgggca gatttacggc atgtatgtcc tgaccgaaac gaatacgacc cgctccgagt
     7621 ttgatcgcta cggtaagtcg aaaaagatag aattttcact gactcttgaa cgctgtgatg
     7681 aggatttgcg ggagcgcctg caatcctcat cgttcagcga tatgttgtcc ggcttcaaag
     7741 ataaggtgac atcatccctt aatagcgcga ccagctcagt taaggggctg ctctgattta
     7801 acgtatgtcg ccaatttcct gatgaaggtg actggcgact tgctgttgta tgtccttctc
     7861 agaaaattgt ttttgaataa caaacaacag gattttataa tc
//
LOCUS       DAZKHF020000203         7887 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2201S31241
            SAMN43267800-rid25106413.denovo.205, whole genome shotgun sequence.
ACCESSION   DAZKHF020000203 DAZKHF020000000
VERSION     DAZKHF020000203.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267800
            Sequence Read Archive: SRR30307263
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 7887)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7887)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 7887)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350581.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350581.2
            Genome Coverage       :: 151x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:21:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,652
            CDSs (total)                      :: 5,557
            Genes (coding)                    :: 5,320
            CDSs (with protein)               :: 5,320
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 237
            CDSs (without protein)            :: 237
            Pseudo Genes (ambiguous residues) :: 0 of 237
            Pseudo Genes (frameshifted)       :: 102 of 237
            Pseudo Genes (incomplete)         :: 130 of 237
            Pseudo Genes (internal stop)      :: 52 of 237
            Pseudo Genes (multiple problems)  :: 41 of 237
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7887
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267800-rid25106413.denovo.205"
                     /serotype="O 26:H11"
                     /isolate="2201S31241"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-01"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            222..764
                     /gene="yfaZ"
                     /locus_tag="ACHOSC_RS18245"
                     /old_locus_tag="pgaptmp_003649"
     CDS             222..764
                     /gene="yfaZ"
                     /locus_tag="ACHOSC_RS18245"
                     /old_locus_tag="pgaptmp_003649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311165.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfaZ family outer membrane protein"
                     /protein_id="HGU4549511.1"
                     /translation="MKKIALAGLAGMLLVSASVNAMSISGQAGKEYTNIGVGFGTEST
                     GLALSGNWTHNDDDGDVAGVGLGLNLPLGPLMATVGGKGVYTNPNYGDEGYAAAVGGG
                     LQWKIGNSFRLFGEYYYSPDSLSSGIQSYEEANAGARYTIMRPVSIEAGYRYLNLSGK
                     DGNRDNAVADGPYVGVNASF"
     gene            864..2066
                     /gene="yfaY"
                     /locus_tag="ACHOSC_RS18250"
                     /old_locus_tag="pgaptmp_003650"
     CDS             864..2066
                     /gene="yfaY"
                     /locus_tag="ACHOSC_RS18250"
                     /old_locus_tag="pgaptmp_003650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinamide mononucleotide deamidase-related
                     protein YfaY"
                     /protein_id="HGU4549512.1"
                     /translation="MLKVEMLSTGDEVLHGQIVDTNAAWLADFFFHQGLPLSRRNTVG
                     DNLDDLVTILRERSQHADVLIVNGGLGPTSDDLSALAAATAKGEGMVLHEAWLKEMER
                     YFHERGRVMAPSNRKQAELPASAEFINNPVGTACGFAVQLNRCLMFFTPGVPSEFKVM
                     VEHEILPRLRERFSLPQPPVCLRLTTFGRSESDLAQSLDTLQLPPGVTMGYRSSMPII
                     ELKLTGPASEQQAMEKLWLDVKRVAGQSVIFEGTEGLPAQISRELQNRQFSLTLSEQF
                     TGGLLALQLSRAGAPLLACEVVPSQEETLAQTAHWITERRANHFAGLALAVSGFENEH
                     LNFALATPDGTFALRVRFSTTRYSLAIRQEVCAMMALNMLRRWLNGQDIASEHGWIEV
                     IESMTLSV"
     gene            2130..2591
                     /locus_tag="ACHOSC_RS18255"
                     /old_locus_tag="pgaptmp_003651"
                     /pseudo
     CDS             2130..2591
                     /locus_tag="ACHOSC_RS18255"
                     /old_locus_tag="pgaptmp_003651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416748.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="aldolase/citrate lyase family protein"
     gene            complement(2632..3534)
                     /gene="rpnE"
                     /locus_tag="ACHOSC_RS18260"
                     /old_locus_tag="pgaptmp_003652"
     CDS             complement(2632..3534)
                     /gene="rpnE"
                     /locus_tag="ACHOSC_RS18260"
                     /old_locus_tag="pgaptmp_003652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ISNCY family transposase"
                     /protein_id="HGU4549513.1"
                     /translation="MTESTTSSPHDAVFKTFMFTPETARDFLEIHLPEPLRKLCNLQT
                     LRLEPTSFIEKSLRAYYSDVLWSVETSDGDGYIYCVIEHQSSAEKNMAFRLMRYATAA
                     MQRHLDKGYDRVPLVVPLLFYHGETSPYPYSLNWLDEFDDPQLARQLYTEAFPLVDIT
                     IVPDDEIMQHRRIALLELIQKHIRDRDLIGMVDRITTLLVRGFTNDSQLQTLFNYLLQ
                     CGDTSRFTRFIEEIAERSPLQKERLMTIAERLRQEGHQIGWQEGMHEQAIKIALRMLE
                     QGIDRDQVLAATQLSEADLAANNH"
     gene            complement(3728..4918)
                     /gene="glpC"
                     /locus_tag="ACHOSC_RS18265"
                     /old_locus_tag="pgaptmp_003653"
     CDS             complement(3728..4918)
                     /gene="glpC"
                     /locus_tag="ACHOSC_RS18265"
                     /old_locus_tag="pgaptmp_003653"
                     /EC_number="1.1.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009331 - glycerol-3-phosphate
                     dehydrogenase (FAD) complex [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004368 - glycerol-3-phosphate
                     dehydrogenase (quinone) activity [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006071 - glycerol metabolic process
                     [Evidence IEA];
                     GO_process: GO:0009061 - anaerobic respiration [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic glycerol-3-phosphate dehydrogenase
                     subunit GlpC"
                     /protein_id="HGU4549514.1"
                     /translation="MNDTSFENCIKCTVCTTACPVSRVNPGYPGPKQAGPDGERLRLK
                     DGALYDEALKYCINCKRCEVACPSDVKIGDIIQRARAKYDTTRPSLRNFVLSHTDLMG
                     SVSTPFAPIVNTATSLKPVRQLLDAALKIDHRRTLPKYSFGTFRRWYRSIAAQQAQYK
                     DQVAFFHGCFVNYNHPQLGKDLIKVLNAMGTGVQLLSKEKCCGVPLIANGFTDKARKQ
                     AITNVESIREAVGVKGIPVIATSSTCTFALRDEYPEVLNVDNKGLRDHIELATRWLWR
                     KLDEGKTLPLKPLPLKVVYHTPCHMEKMGWTLYTLELLRKIPGLELTVLDSQCCGIAG
                     TYGFKKENYPTSQAIGAPLFRQIEESGADLVITDCETCKWQIEMSTSLRCEHPITLLA
                     QALA"
     gene            complement(4915..6174)
                     /gene="glpB"
                     /locus_tag="ACHOSC_RS18270"
                     /old_locus_tag="pgaptmp_003654"
     CDS             complement(4915..6174)
                     /gene="glpB"
                     /locus_tag="ACHOSC_RS18270"
                     /old_locus_tag="pgaptmp_003654"
                     /EC_number="1.1.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004368 - glycerol-3-phosphate
                     dehydrogenase (quinone) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate dehydrogenase subunit GlpB"
                     /protein_id="HGU4549515.1"
                     /translation="MRFDTVIMGGGLAGLLCGLQLQKHGLRCAIVTRGQSALHFSSGS
                     LDLLSHLPDGQPVTDIHSGLESLRQQAPAHPYSLLEPQRVLDLACQAQALIAESGAQL
                     QGSVELAHQRVTPLGTLRATWLSSPEVPVWPLPAKKICVVGISGLMDFQAHLAAASLR
                     ELDLSVETAEIELPELDVLRNNATEFRAVNIARFLDNEENWPLLLDALIPVANTCEMI
                     LMPACFGLADDKLWRWLNEKLPCSLMLLPTLPPSVLGIRLQNQLQRQFVRQGGVWMPG
                     DEVKKVTCKNGVVNEIWTRNHADIPLRPRFAVLASGSFFSGGLVAERNGIREPILGLD
                     VLQTATRGEWYKGDFFAPQPWQQFGVTTDQTLRPSQAGQTIENLFAIGSVLGGFDPIA
                     QGCGGGVCAVSALHAAQQIAQRAGGQQ"
     gene            complement(6164..7792)
                     /gene="glpA"
                     /locus_tag="ACHOSC_RS18275"
                     /old_locus_tag="pgaptmp_003655"
     CDS             complement(6164..7792)
                     /gene="glpA"
                     /locus_tag="ACHOSC_RS18275"
                     /old_locus_tag="pgaptmp_003655"
                     /EC_number="1.1.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009331 - glycerol-3-phosphate
                     dehydrogenase (FAD) complex [Evidence IEA];
                     GO_function: GO:0004368 - glycerol-3-phosphate
                     dehydrogenase (quinone) activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0006072 - glycerol-3-phosphate metabolic
                     process [Evidence IEA];
                     GO_process: GO:0046174 - polyol catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic glycerol-3-phosphate dehydrogenase
                     subunit A"
                     /protein_id="HGU4549516.1"
                     /translation="MKTRDSQSSDVIIIGGGATGAGIARDCALRGLRVILVERHDIAT
                     GATGRNHGLLHSGARYAVTDAESARECISENQILKRIARHCVEPTNGLFITLPEDDLS
                     FQATFIRACEEAGISAEAIDPQQARIIEPAVNPALIGAVKVPDGTVDPFRLTAANMLD
                     AKEHGAVILTAHEVTGLIREGATVCGVRVRNHLTGETQALHAPVVVNAAGIWGQHIAE
                     YADLRIRMFPAKGSLLIMDHRINQHVINRCRKPSDADILVPGDTISLIGTTSLRIDYN
                     EIDDNRVTAEEVDILLREGEKLAPVMAKTRILRAYSGVRPLVASDDDPSGRNVSRGIV
                     LLDHAERDGLDGFITITGGKLMTYRLMAEWATDAVCRKLGNTRPCTTADLALPGSQEP
                     AEVTLRKVISLPAPLRGSAVYRHGDRTPAWLSEGRLHRSLVCECEAVTAGEVQYAVEN
                     LNVNSLLDLRRRTRVGMGTCQGELCACRAAGLLQRFNVTTSAQSIEQLSTFLNERWKG
                     VQPIAWGDALRESEFTRWVYQGLCGLEKEQKDAL"
BASE COUNT         1774 a         2049 c         2172 g         1892 t
ORIGIN      
        1 tgaaagtgac agtaatctct tttgcaatga taactgcact tattattatt tttgtgtgta
       61 tcggtaaagt gtgtttattt tttcggcgtt taattattta agaagtattt agttacaata
      121 agtgactaga tccctttttt cgctcattga atcctctgtg ccaggcagta caatcccgca
      181 gctttgggaa ttttgattaa ttgcttgtgg gaaaggggat aatgaaaaaa attgcgctag
      241 caggtctggc cggaatgctg ttggtttctg catcggtcaa tgcaatgagc atcagcggcc
      301 aggcgggtaa agaatacacc aatattggtg tcggttttgg gactgaatcg acgggcctgg
      361 ctttaagcgg taactggaca cataacgacg acgacggtga cgtcgcgggc gtggggctgg
      421 ggttgaatct gcctctcggg ccgttaatgg cgaccgttgg cggaaaaggc gtttacacca
      481 acccgaatta cggcgatgaa ggttatgccg cagcggtagg aggtggtttg cagtggaaaa
      541 ttggcaacag cttccgtttg tttggcgagt attactactc tccggattcg ctctccagcg
      601 gtattcaaag ttatgaggaa gcgaatgctg gcgcgcgtta caccattatg cgtccagtca
      661 gtattgaggc gggttatcgc tacctgaatc tgtcgggtaa agacggtaac cgcgacaacg
      721 ccgtggctga cggcccgtat gttggggtta acgccagttt ctgatccctt ccggcgcggc
      781 attctgtcgc gccattcagt ttcttactac gccttatctc cctttgcgtt atagtgattc
      841 gataacaaaa gcaggagata accatgttaa aagtggaaat gctatccacc ggggatgaag
      901 tgttacacgg gcaaatcgtt gacactaacg ctgcctggct ggccgatttt ttctttcatc
      961 aggggctgcc attatctcgc cgcaatacgg tgggggataa ccttgatgac ttagtcacca
     1021 ttcttcgcga acgtagtcag cacgccgatg tgctgatcgt taacggcggg ctgggaccga
     1081 ccagcgatga tttaagcgca ctcgccgctg cgacagcaaa aggtgaaggc atggtgctgc
     1141 atgaagcctg gctcaaagag atggaacgct atttccacga acgtggacga gtaatggcac
     1201 cgagcaaccg taaacaagcg gagctgcctg ccagtgctga atttatcaat aacccggtag
     1261 gcaccgcctg tggttttgcc gtgcagctta atcgttgcct gatgttcttt actcccggcg
     1321 taccgtcaga atttaaggtg atggtcgagc acgaaatcct gccgcgtctg cgcgagcgtt
     1381 tttctttacc acagccgccg gtttgtctgc gtttaactac ctttggtcgt tcggaaagcg
     1441 atctggcaca aagcctggac actctacaac tgccgccggg cgtaacaatg ggctatcgct
     1501 cttcaatgcc gattatcgaa ctgaaactca ccggaccggc aagcgagcaa caggcgatgg
     1561 aaaaactgtg gctggacgtt aaacgtgttg ccggacagag cgtgattttc gaaggcaccg
     1621 aaggactgcc cgcgcagatc agtcgcgaat tgcaaaaccg ccagttcagc ctgacgttga
     1681 gcgagcaatt caccggtggt ttattggctt tgcaactttc tcgcgcaggt gctccattgc
     1741 tggcgtgtga agtggttcct tcacaggaag aaaccctggc gcaaactgcg cactggatta
     1801 cagaacggcg ggccaaccat tttgccgggc tggcactggc tgtttcgggt ttcgagaacg
     1861 aacatctcaa ctttgcgcta gccacaccag acggcacttt cgctctgcgt gtgcgtttca
     1921 gcactacgcg ctacagcctg gctatccgtc aggaagtgtg cgcaatgatg gcactgaata
     1981 tgctgcgccg ttggttaaac ggccaggata tcgccagtga gcatggctgg attgaggtta
     2041 ttgagtccat gaccttatct gtctgattcc gccactctgt gccggtttac tgcaccggca
     2101 cattccccct ccggcaaaac ttgtccaacc ctccctacgg tgagcgtggt gttggggcca
     2161 gtgtggcacg ggctgcgcgc tggggacgca ttgagaatta catggcgcaa gtcaacgatt
     2221 cgctttgtct gttagtgcag gtggaaagta aaacggcact ggataacctg gacgagatcc
     2281 tcgacgtcga agggattgat ggcgtgttta ttggaccagc ggatctttct gcgtcgttgg
     2341 gctacccgga taacgccggg cacccggaag tgcagcgaat tattgaaacc agcattcggc
     2401 ggatccgtgc tgcgggtaaa gcggctggtt ttctggctgt ggctcctgat atggcgcagc
     2461 aatgcctggc gtggggagcg aactttgtcg ctgttggcgt tgacacgatg ctctacagcg
     2521 atgccctgga tcaacgactg gcgatgttta aatcaggcaa aaatgggcca cgcataaaag
     2581 gtagttattg atatcaaagg cccatgggga tcggctgtgg gcctgtgtta attagtggtt
     2641 attcgctgcc agatcggctt cgcttagctg ggtggccgcg agcacctggt cacgatcaat
     2701 gccctgttcc agcatgcgca aagcaatttt aatggcttgt tcatgcatac cttcctgcca
     2761 gccaatttga tgcccttcct gccgtagccg ttcagcaata gtcattaatc tctccttttg
     2821 tagtggtgaa cgttcggcaa tctcctcaat aaaacgggtg aaacgggagg tatcgccgca
     2881 ttgcagcaga taattaaaca gtgtttgtag ctggctgtca ttagtgaagc ctctaaccaa
     2941 aagcgtggtg atcctgtcga ccatgccgat taaatcgcgg tcgcgaatat gcttttgaat
     3001 cagttccagc agagctatac gccgatgttg catgatctca tcgtcaggta cgatggtgat
     3061 atccaccaac ggaaaagctt cggtgtacaa ctgccgggca agttgcggat cgtcaaactc
     3121 atccagccag ttaagtgagt acgggtaggg cgatgtttcg ccatgataaa acagcaatgg
     3181 caccaccagc ggaactctgt catagccttt atccaggtga cgctgcatgg cggcagtggc
     3241 atagcgcatt agccgaaaag ccatattctt ttctgcagag ctttgatgtt caatcacgca
     3301 gtagatatag ccgtcaccgt cgctggtttc cacggaccac aaaacatccg agtagtaagc
     3361 gcgtaaactt ttttcaataa aactagtggg ttccaggcgt aaggtttgca ggttgcaaag
     3421 cttgcgcagt ggttctggta aatgtatttc gagaaaatcc cgtgcggttt cgggtgtgaa
     3481 cataaaggtt ttaaacaccg catcatgcgg ggaggatgtt gttgattcgg tcatggtttt
     3541 ccgtcacctg atatttatga tgacgcgaca ttacgtctat cccgggcctt tgacatctgt
     3601 cagtttcagc ctttgcgaga cattttcaga ggaattttca aagcgttgca acgcatacat
     3661 ggtgcgtgga atcgcgctcg caaaaatgcc cgataagcaa tgtgcttacc gggcatcaaa
     3721 aggtcggtta agccagcgcc tgggccagta gcgtaatcgg atgttcgcag cgaaggctcg
     3781 tggacatctc aatctgccat ttgcaggttt cgcagtcggt gatgaccaga tccgcgccgc
     3841 tctcttctat ctggcggaac agtggcgcac cgatggcttg tgaggtgggg tagttctctt
     3901 ttttgaaacc gtaagtaccc gcaataccgc agcactggga atccagcacc gttaactcaa
     3961 gccccgggat tttacgcaac agctccaggg tgtagagcgt ccagcccatt ttttccatat
     4021 ggcacggagt gtgataaacc actttcagcg gcagcggttt cagcggtaac gttttgcctt
     4081 cgtccagctt gcgccacagc cagcgggttg ccagttcgat atgatcgcgc aagcctttgt
     4141 tgtcgacatt cagcacttcc gggtattcgt cgcgcagggc aaatgtacag gttgaggagg
     4201 tggcaatcac cggaatgcct tttactccca cagcttcgcg gatcgactct acattcgtaa
     4261 ttgcctgttt gcgtgcttta tcggtaaagc cgttggcgat tagcggtacg ccgcagcatt
     4321 tttctttgct gagcagttgt acaccggtac ccattgcgtt gagcacttta attaaatctt
     4381 tacctaactg cggatggttg tagttaacga agcagccgtg aaagaaagcg acctggtctt
     4441 tatattgtgc ttgctgagcc gctatgctgc gataccagcg acgaaacgta ccgaaggagt
     4501 atttcggtag cgtgcggcga tgatcgattt ttaacgccgc atcaagcagc tgccgcaccg
     4561 gtttcagcga ggtagcggtg ttgacgattg gtgcgaacgg cgtggaaacg ctacccatca
     4621 ggtcggtatg actcaacaca aaattacgca gcgacgggcg cgtggtgtca tattttgccc
     4681 gcgcgcgctg gataatatcg ccaatcttca catcggacgg gcaggcgact tcacaacgtt
     4741 tgcagttgat gcaatatttc agcgcctcgt catacagtgc gccatctttc aaacgcagac
     4801 gctcgccatc cggcccggct tgttttggcc ctggataacc gggattcacc cggctcaccg
     4861 ggcaggcggt ggtgcagacg gtgcacttaa tgcagttttc gaagctggtg tcgttcattg
     4921 ttggcctcct gcgcgttggg caatctgttg agcggcatgt aaagcactga cggcacaaac
     4981 accgccgccg catccctggg cgatgggatc aaatccgccc agcaccgaac cgatggcaaa
     5041 caggttctca atggtttgcc ctgcctgcga cgggcgtagc gtctgatcag tggttacacc
     5101 gaactgctgc cacggttgcg gcgcaaaaaa atcgccctta taccattcac cccgcgtggc
     5161 ggtttgtagc acatcaaggc cgagaatcgg ctcgcgaatg ccgttacgtt ccgctaccag
     5221 tccgccacta aagaaactgc cgctggcgag aaccgcgaaa cgtggacgta gcggaatatc
     5281 ggcgtgattg cgggtccaga tttcattcac tacgccattt ttacaggtca cttttttcac
     5341 ttcatcgccc ggcatccaca cgccaccctg gcgcacaaac tggcgctgta actggttttg
     5401 cagacgaatg cccagcacgg aaggcggcag cgttggcaaa agcatcagtg aacaaggtag
     5461 tttttcattc aaccaacgcc acagtttgtc atcggccagg ccgaagcagg cgggcatcag
     5521 gatcatttcg caggtattgg cgacaggaat aagcgcatca agtaacagcg gccagttttc
     5581 ttcattatca aggaaacggg cgatattcac cgcgcgaaat tcggtggcgt tattgcgcag
     5641 gacatccagt tccggcagct ctatttctgc ggtttcaacc gaaagatcca gttcgcgcaa
     5701 cgaagccgcc gcaagatgcg cctggaaatc catcagcccg cttatcccca ctacacatat
     5761 cttcttcgcg ggtagcggcc agacgggaac ttctggcgaa cttaaccagg ttgcacgcaa
     5821 agtgccgagc ggggtaacgc gctgatgggc cagttccacg ctgccctgca attgcgcacc
     5881 gctttcagcg atcaatgcct gcgcctggca agcgagatcg agcacgcgtt gtggttcgag
     5941 aagactgtaa ggatgggctg gtgcctgctg acgcaaagat tccagtccac tgtgaatgtc
     6001 tgtcaccggt tgaccatcag gcagatggct cagcaaatcc agcgaaccgg atgagaaatg
     6061 cagcgcgctt tgaccacgcg tgacaatggc acagcgcagg ccgtgttttt gcagttgcag
     6121 gccacagagt aatccggcga ggccgccgcc cataatgaca gtatcaaagc gcatctttct
     6181 gctccttctc cagaccacac aatccctgat aaacccagcg ggtaaattcg ctttcgcgca
     6241 gtgcatctcc ccaggcgatg ggttgcacgc ctttccagcg ttcgttaagg aaggtggaaa
     6301 gttgctcgat agattgcgcg gacgtcgtga cgttaaaacg ttgcagcagt ccggcagcgc
     6361 ggcaggcgca gagttcgccc tggcaggtac ccatccccac acgggtacga cggcgtaaat
     6421 ccagcaggct attaacgttt aaattttcta ccgcgtactg aacttcaccc gcagttaccg
     6481 cttcgcactc acataccagg ctacgatgca gacggccttc gctcagccag gcaggcgtgc
     6541 gatcgccatg acgataaacc gcagaaccgc gcagcggggc aggcagggag atgactttac
     6601 gcaaggtaac ttcagcgggt tcttgtgaac caggcagtgc cagatcggca gtcgtacagg
     6661 ggcgcgtgtt gcccagtttg cggcataccg cgtcggtagc ccattcagcc atcagccgat
     6721 aggtcatcag tttgccaccg gtgatggtga taaatccgtc cagaccatcg cgttcagcat
     6781 ggtcgagcag cacgatgcca cggctgacgt tacgtccgct cgggtcgtca tcgctggcaa
     6841 ccagcgggcg cacgccagaa taggcccgca aaatgcgcgt tttcgccatc acgggggcca
     6901 gtttttcccc ttcacgcagc agaatatcaa cctcttctgc cgtcactcga ttatcgtcaa
     6961 tctcgttgta atcaatacgt aaagaggtgg tgccaatcag cgaaatggta tcgccaggca
     7021 ccagaatatc ggcgtcggaa ggtttacggc agcggttgat cacatgctgg ttaatgcggt
     7081 gatccatgat cagcagcgat cctttcgccg ggaacatgcg aatgcgcaga tcggcatatt
     7141 cggcaatgtg ttgcccccag atcccagcgg cattaaccac gacaggtgca tgaagggcct
     7201 gagtttcgcc ggtgagatgg ttacgtacac gaacaccgca caccgtcgcg ccttcacgaa
     7261 tcagccccgt gacttcatga gcggtaagga taacggcacc gtgttctttg gcatccagca
     7321 tgtttgctgc ggtcagacga aatggatcaa cggtgccatc cggaactttc accgcgccaa
     7381 tcagtgccgg gttaacggca ggttcgataa tgcgcgcttg ctgcgggtct atagcttctg
     7441 cgctgatccc tgcttcttcg caggcgcgaa taaaagtggc ctggaatgag aggtcatctt
     7501 ccggcagggt gataaacagg ccgttggttg gttcaacgca gtgacgtgca atgcgtttca
     7561 ggatctggtt ttcactaatg cattcgcggg ccgattccgc atcggttacc gcatagcgcg
     7621 caccgctgtg cagcaggccg tggttacgcc cggtggcacc ggttgcgatg tcgtggcgct
     7681 caaccaaaat cacgcgcagc ccgcgcaggg cacagtcgcg ggcaatcccg gctcccgttg
     7741 cgccgccacc aatgataatc acgtcacttg attgcgagtc gcgagttttc attgttatcc
     7801 ctctgaagtt cgttttttac catttagcca tagtaaaaac atgaattgtt tgatttcgcg
     7861 catatccgct cataattcga aagtgaa
//
LOCUS       DAZKHF020000204         7829 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2201S31241
            SAMN43267800-rid25106413.denovo.206, whole genome shotgun sequence.
ACCESSION   DAZKHF020000204 DAZKHF020000000
VERSION     DAZKHF020000204.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267800
            Sequence Read Archive: SRR30307263
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 7829)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7829)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 7829)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350581.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350581.2
            Genome Coverage       :: 151x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:21:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,652
            CDSs (total)                      :: 5,557
            Genes (coding)                    :: 5,320
            CDSs (with protein)               :: 5,320
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 237
            CDSs (without protein)            :: 237
            Pseudo Genes (ambiguous residues) :: 0 of 237
            Pseudo Genes (frameshifted)       :: 102 of 237
            Pseudo Genes (incomplete)         :: 130 of 237
            Pseudo Genes (internal stop)      :: 52 of 237
            Pseudo Genes (multiple problems)  :: 41 of 237
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7829
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267800-rid25106413.denovo.206"
                     /serotype="O 26:H11"
                     /isolate="2201S31241"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-01"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            62..5237
                     /locus_tag="ACHOSC_RS18280"
                     /old_locus_tag="pgaptmp_003656"
                     /pseudo
     CDS             62..5237
                     /locus_tag="ACHOSC_RS18280"
                     /old_locus_tag="pgaptmp_003656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016244610.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ECSE_1600 family autotransporter"
     gene            5460..5726
                     /gene="hipB"
                     /locus_tag="ACHOSC_RS18285"
                     /old_locus_tag="pgaptmp_003657"
     CDS             5460..5726
                     /gene="hipB"
                     /locus_tag="ACHOSC_RS18285"
                     /old_locus_tag="pgaptmp_003657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system antitoxin HipB"
                     /protein_id="HGU4549517.1"
                     /translation="MMSFQKIYSPTQLANAMKLVRQQNGWTQSELAKKIGIKQATISN
                     FENNPDNTTLTTFFKILQSLELSMTLCDTKNASPESTEQQDLEW"
     gene            5726..7048
                     /gene="hipA"
                     /locus_tag="ACHOSC_RS18290"
                     /old_locus_tag="pgaptmp_003658"
     CDS             5726..7048
                     /gene="hipA"
                     /locus_tag="ACHOSC_RS18290"
                     /old_locus_tag="pgaptmp_003658"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system serine/threonine
                     protein kinase toxin HipA"
                     /protein_id="HGU4549518.1"
                     /translation="MPKLVTWMNNQRVGELTKLANGAHTFKYAPEWLASRYARPLSLS
                     LPLQRGNITSDAVFNFFDNLLPDSPIVRDRIVKRYHAKSRQPFDLLSEIGRDSVGAVT
                     LLPENETITRPIMAWEKLTEARLEEVLTAYKADIPLGMIREENDFRISVAGAQEKTAL
                     LRIGNDWCIPKGITPTTHIIKLPIGEIRQPNATLDLSQSVDNEYYCLLLAKELGLNVP
                     DAEIIKAGNVRALAVERFDRRWNTERTVLLRLPQEDMCQTFGLPSSVKYESDGGPGIA
                     RIMAFLMGSSEALRDRYDFMKFQVFQWLIGATDGHAKNFSVFIQAGGSYRLTPFYDII
                     SAFPVLGGTGIHISDLKLAMGLNASKGKKTAIDKIYPRHFLATAKVLRFPEVQMHEIL
                     SDFARMIPAALDNVKTSLPTDFPENVVTAVETNVLRLHGRLSREYGSK"
     gene            7360..7568
                     /locus_tag="ACHOSC_RS18295"
                     /old_locus_tag="pgaptmp_003659"
                     /pseudo
     CDS             7360..7568
                     /locus_tag="ACHOSC_RS18295"
                     /old_locus_tag="pgaptmp_003659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005226947.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
BASE COUNT         2146 a         1663 c         2135 g         1885 t
ORIGIN      
        1 tttttaatgt ctgacgactc atctctttcg tggctgtatt ttttgtatat ggagcgttta
       61 tatgaataga atctatcgcg tgatatggaa ttgcactcta caggtatttc aggcctgctc
      121 ggaattaact cgcagggcag gtaaaacatc gacggttaat ttgcgtaaat cctctggact
      181 gacaacgaaa ttcagtagat tgacgctggg tgttttgctg gcactaagcg gttcagcgtc
      241 tggtgcaagt ctggaagttg ataatgatca gattaccaat attgatactg atgttgctta
      301 tgatgcctac ctggttggct ggtatggcac tggagtgctt aatattttgg ctggcggtaa
      361 tgcctcctta accactatta ctaccagcgt cattggcgct aatgaggact cagagggcac
      421 cgttaatgtt ttgggtggca cctggcgatt gtatgatagc ggaaataatg caaggccttt
      481 aaatgtgggt caatccggaa cggggacgct gaatattaaa cagaagggtc acgtcgatgg
      541 aggctattta agattaggtt cttcgacagg aggcgtcggg acggtcaatg ttgagggaga
      601 ggactctgtt ctgacgaccg aattattcga aatagggagt tacggtacgg gctcattaaa
      661 tattacggat aagggttacg tcacgagttc aatcgtcgcc attttaggct atcaggcggg
      721 cagtaatggc caggttgtcg ttgaaaaggg tggcgagtgg ctaataaaaa ataacgattc
      781 ctcaattgaa tttcaaattg gtaatcaagg aactggggag gcgactattc gcgagggtgg
      841 actgattacg gctgaaaata cgattatcgg tggcaatgcc accggtgtcg gaaccctgaa
      901 tgtgcaggat caagactctg tcatcacggt acgcagactc tataatggat atttcggtaa
      961 tggcacactc aatatttcca ataatggact aattaataac aaagaatatt cattagtggg
     1021 cgttcaggac ggttcccacg gtgtcgtcaa cgtgaccgat aaagggcatt ggaatttcct
     1081 cggaacgggc gaagctttcc gctatatcta tatcggtgat gctggcgacg gtgaacttaa
     1141 tgtctcgcgt gaaggtaaag tagattcggg aattatcact gcggggatga aagaaacagg
     1201 cacaggcaac attactgtta aggataagaa ctccgttatc actaatctcg gaactaatct
     1261 tggttatgac ggccacggcg aaatgaatat cagtaatgaa gggcttgttg tcagcaacgg
     1321 aggaagctca ctcggttatg gagaaaccgg cgtcgggaat gtcagcatca ccacgggggg
     1381 gatgtgggag gtcaataaga atgtctatac caccattggt gttgcgggcg tcggaaacct
     1441 caatattagc gatggcggta agttcgtatc gcaaaatatt acttttttgg gcgataaagc
     1501 aagcggtatc ggcacactga acctgatgga tgcgacatca tcgttcgata ctgtgggtat
     1561 caatgtcggt aattttggta gcggtatcgt aaatgtcagt aatggtgcca cccttaattc
     1621 aacgggctat ggatttatcg gaggaaatgc ctccggtaag gggatagtta atatttcaac
     1681 ggacagtctc tggaatttaa agacatcatc tactaacgca caattgctac aggtcggtgt
     1741 attaggcaag ggtgaactga atattaccac cggaggtata gttaaagcgc gtgatacaca
     1801 gatagctctc aatgacaaaa gtaagggcga cgtgagggtg gatgggcaga actctcttct
     1861 tgaaacattc aatatgtacg tagggacatc tggtacgggt acgttaaccc tgacgaatag
     1921 cggcacgctg aatgtcgaag gtggagaagt ttacttaggt gtttttgaac ctgctgtagg
     1981 aacgctaaac attggtgcag ctcacggtga ggcggcggca gatgccgggt atataaccaa
     2041 cgcgacgaaa gtagagtttg gttctggcga aggtgttttt gtctttaatc atactaataa
     2101 cagtgatgcc ggctaccagg tcgatatgct gattacaggt gacgataaag acggaaaagt
     2161 gatccatgat gcaggccata cggtgttcaa tgcagggaat acttatagcg gtaaaacgct
     2221 ggtcaatgac ggcctcctga ccattgcgtc tcatacggca gatggggtaa cgggcatggg
     2281 ttcgagtgaa gtcaccattg caagccccgg tacgctcgac attctcgcat caacgaacag
     2341 tgcaggagat tacacgctga ccaatgcgct caaaggcgat ggcttgatgc gagtgcagct
     2401 gtcatcctac gacaagatgt ttggctttac ccatgcaaca gggactgaat tcgccggtgt
     2461 tgcccaactg aaagacagta ccttcactct ggaacgcgac aacaccgctg cgcttactca
     2521 cgcgatgttg cagtctgaca gtgaaaatac cacatcggta aaagtgggag agcaatccat
     2581 tggtggactg gccatgaatg gcggtacgct cattttcgat acggatattc ctgctgcgac
     2641 gcttgcagag ggatatatca gcgtcgatac gctggttgtc ggcgcgggtg actacacctg
     2701 gaaaggccgt aactatcagg taaacgggac gggcgacgtg cctaaaccgt ggaatgatcc
     2761 catggcgaat aaccctctga cgacgctcaa tttgctggaa cacgatgata gccatgtcgg
     2821 cgttcaactg gtgaaggcgc aaacggttat tgggtcgggt ggctcattaa cgttacgtga
     2881 tttacagggc gacgaggtgg aagcggacaa aacgttacac attgcgcaaa acggaacggt
     2941 ggtcgccgag ggtgattatg gattccgcct cacgaccgca ccaggtgatg gtttgtacgt
     3001 taactatggg ctgaaagcgc tgaacatcca tggtgggcaa aagctgacgt tagccgaaca
     3061 tggcggagcc tatggcgcaa cggccgatat gtcggcaaaa atcggtggtg aaggggatct
     3121 ggcaatcaat acggtgcgac aggtttcgct ttccaacggt cagaacgact atcagggggc
     3181 aacctacgtt cagatgggga cattacgtac cgatgcggat ggtgcacttg gcaacacccg
     3241 ggaactgaac atcagcaacg cggccatcgt cgatcttaat ggatcgacgc agacggtaga
     3301 gacattcacc gggcagatgg gttcgactgt tttgttcaaa gagggggcgc tgacggtaaa
     3361 taaaggtggg atcagtcagg gtgaactgac aggtggcgga aacctgaatg ttacaggggg
     3421 aacgctggct atcgaggggc ttaatgcacg ctacaatgcg ttaaccagca ttagcccaaa
     3481 tgcggaagtc agcctcgata atacgcaggg gttaggcaga ggaaatattg ccaataacgg
     3541 tctgttaacg ctaaaaaacg tgactggcga actgcgcaat agcataagcg ggaagggtat
     3601 cgtgagcgca accgccagga cagatgtaga gctggatggc gataatagcc gctttgtggg
     3661 gcaattcaac attgatacag gcagcgcgct cagcgtcaac gagcagaaaa acctgggtga
     3721 tgcttccgtt atcaataatg gcctgctcac catctccact gagcgtagct gggcgatgac
     3781 gcacagtatc agcggtagcg gtgatgtgac aaaactgggt accgggatcc tgactcttaa
     3841 caacgattcc gcggcgtatc agggtacgac ggatatcgtg gggggggaaa ttgctttcgg
     3901 ttccgactct gccattaata tggcaagtca acacattaat atccataaca gcggtgtgat
     3961 gtcgggaaat gtcaccactg caggtgatgt gaacgttatg cctgggggga cactgcgtgt
     4021 cgctaaaacc acaatcggcg gcaacctgga gaatggcggc acggttcaaa tgaatagcga
     4081 aggggggaaa ccggggaatg tactgaccgt taacggcaac tataccggaa acaatggcct
     4141 gatgacgttc aacgcgacgc tgggcggcga taattcgccc accgataaaa tgaacgtgaa
     4201 aggcgatacc caagggaaca ctcgcgttcg ggttgataac attggcggcg tcggtgcaca
     4261 aacggtcaac ggtattgaac tcattgaggt tggcggtaat tctgcaggta acttcgcgct
     4321 gaccaccgga actgtcgaag ctggggctta cgtctacacg ctggctaaag ggaaggggaa
     4381 tgacgagaaa aactggtatc tgaccagtaa atgggacggc gtaacgccag cggatacacc
     4441 cgatcccatc aataatcccc ctgttgtgga tccggaaggc ccatcagttt atcgcccgga
     4501 ggccggaagc tatatcagca acattgccgc agccaactcg ctgtttagcc atcgtttaca
     4561 cgaccgtctg ggtgagccgc agtatataga ttcactgcat tctcaggggt cggcaagcag
     4621 tatgtggatg cgtcatgtcg gagggcacga acgttcaagg gccggtgacg gtcagctaat
     4681 tactcaggct aaccgctatg tattgcagct aggcggcgat ttggcgcagt ggagtagcaa
     4741 cgcgcaggat cgctggcatg acggcctctg ttgagggtgg gtataccttt gaagcgggaa
     4801 catttagagg cagcgaaggg acgctgaata cctggtacgt ccagccacag gcgcaaatca
     4861 cctggatggg tgtgaaagat tctgaccata atcggaaaga cggaacacgc attgaaacgg
     4921 aaggcgacgg aaacgtgcaa acgcgacttg gggtgaaaac ctacctgaat agccatcaca
     4981 agcgtgacga tggtaaacag cgtgagttcc agccttacat tgaagcgaac tggatcaaca
     5041 atagcaaagt ctacgccgtg aagatgaatg gtcaaaccgt aagccgtgat ggtgcgcgta
     5101 atctcggtga agtacgtacc ggggttgagg cgaaagtaaa taacaacctt agcctgtggg
     5161 ggaatgtcgg tgtgcaacta ggtgataaag gctatagcga tactcagggc atgctgggag
     5221 tgaaatatag ctggtaaacc gtataagccg catgtcgaga tggcatgcgg cttaatattg
     5281 ccgacttcaa acggcgcatc agcgcctttt ttaaatcctc ctttttatcc gcgatcgcgg
     5341 atatcgcagc gtttatcccg tagagcggat aagacgtgtt tccaaattga cttatcctca
     5401 ctaaaggata aaactgataa tatcctctta tgcggataaa tttgctgtgg acgtatgaca
     5461 tgatgagctt tcagaagatc tatagcccaa cgcaattggc gaatgcaatg aaactggttc
     5521 gccagcaaaa tggctggacg cagagcgagc tggcgaaaaa aattggcatt aagcaggcga
     5581 caatttccaa tttcgaaaac aaccctgaca ataccacgct cacgacattt tttaagattt
     5641 tacagtcgct tgaactctca atgacactat gcgacacgaa aaatgcctcg ccagaatcaa
     5701 cagaacagca agatctggag tggtaatgcc taaacttgtc acttggatga acaaccagcg
     5761 ggtaggcgag ttaacgaagt tagccaacgg cgcgcacacc tttaagtatg caccggagtg
     5821 gttagccagc cgttatgcca gaccgttgtc actttcgctg ccattgcaga gggggaatat
     5881 cacctcagat gccgtattta acttcttcga taacctgtta cccgatagcc cgattgtacg
     5941 tgaccggatc gttaaacgtt atcatgctaa atccagacaa ccgtttgatt tattgtcaga
     6001 aatagggcga gacagcgttg gtgccgtaac gttattaccc gaaaatgaaa ccataacgcg
     6061 cccgataatg gcatgggaaa agcttactga agccaggctt gaagaagtat taacggctta
     6121 taaagcagat atccctctgg gcatgattag agaagaaaat gactttcgca tctcggttgc
     6181 tggcgcgcag gagaagacag cgctgcttag aataggcaat gactggtgca ttccgaaagg
     6241 aataacgccg acgacgcaca tcattaaatt accgattggc gaaatcagac agcccaatgc
     6301 gacgctcgat ctcagccaaa gcgttgataa tgagtattac tgtctgctgc tggcgaaaga
     6361 acttgggttg aatgttccgg acgcagaaat cattaaagcg ggaaatgtgc gcgcgttagc
     6421 ggtcgaacgt tttgacaggc gttggaatac tgagcgaacg gttttacttc gcttgccaca
     6481 ggaggatatg tgtcagacat tcggtttacc ttcatctgtg aaatatgaat cagatggagg
     6541 cccaggcatc gcgcggatca tggcgttttt gatggggtcc agcgaggcgc ttcgcgatcg
     6601 ttatgatttt atgaaattcc aggtcttcca gtggttgatt ggcgcaacgg atggccatgc
     6661 aaaaaacttc tccgtattta ttcaggctgg cggcagttat cgactcacgc cattttacga
     6721 catcatttca gcatttccgg tccttggcgg tacgggaata cacatcagcg atctcaaact
     6781 ggcaatgggg cttaacgcat ccaaaggcaa aaaaacggca atcgataaaa tttatccgcg
     6841 acattttttg gcgacagcaa aggtgctgag attcccggaa gtgcagatgc atgaaatcct
     6901 aagtgacttt gccagaatga ttccggcagc actggataac gtgaagactt cattaccgac
     6961 agattttccg gagaacgtgg tgacggcagt tgaaaccaat gtgttgaggt tgcatggacg
     7021 gttaagccga gaatacggta gtaagtgaga tatggggttt ggtcgttgtt aatgagcacg
     7081 acaatcatga ccgccaacga ctggattctt ccttggttta tactggtgcg cataatgtat
     7141 attatgttaa attatgtctg agtgatgcga agtgcttttc tggtagtcgt tattcgttca
     7201 gggttcaaga acttaagcct ctcctctttc ataattggcc tgtgcatgga gaaatagcct
     7261 ggttattccc tgccccatgc taagagcgca gtgtctgtcg catagacact gcatacgaaa
     7321 acaccagagg gtgctgttac ttatttaagg acggatcaag cgggaatgag tccactccgg
     7381 tatcagaaat ggctacgtct caatgaagtc aggcgaccga tgctgaatga acattacgat
     7441 gtcaccacag cagcctatgc tgtcggttac gaaagctatc ccatttcagt cgggaatatt
     7501 cgcggatgtt tggagagtca cccaagagag atattaccag gttaagaaag tctgtcggtc
     7561 agctttaata aaccctgcac ttatctgttt agagcaattc ggtgttagtt tcagcaagca
     7621 aacattaacc atagctaatg atttatagcc atattaacca ttggtagccc ttgacctttc
     7681 ctgacacttc ccttgttcgc aataatattc tgtttagtca cggtttataa ataacatgca
     7741 taatgaaatc gtcatgttat ttagcgacat aaaatgtttt tgcaaatact ctcttcaaca
     7801 agtgcacaat aaacttgttg tgattaggt
//
LOCUS       DAZKHF020000205         7807 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2201S31241
            SAMN43267800-rid25106413.denovo.207, whole genome shotgun sequence.
ACCESSION   DAZKHF020000205 DAZKHF020000000
VERSION     DAZKHF020000205.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267800
            Sequence Read Archive: SRR30307263
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 7807)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7807)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 7807)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350581.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350581.2
            Genome Coverage       :: 151x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:21:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,652
            CDSs (total)                      :: 5,557
            Genes (coding)                    :: 5,320
            CDSs (with protein)               :: 5,320
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 237
            CDSs (without protein)            :: 237
            Pseudo Genes (ambiguous residues) :: 0 of 237
            Pseudo Genes (frameshifted)       :: 102 of 237
            Pseudo Genes (incomplete)         :: 130 of 237
            Pseudo Genes (internal stop)      :: 52 of 237
            Pseudo Genes (multiple problems)  :: 41 of 237
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7807
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267800-rid25106413.denovo.207"
                     /serotype="O 26:H11"
                     /isolate="2201S31241"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-01"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            <1..519
                     /gene="lpfA"
                     /locus_tag="ACHOSC_RS18300"
                     /old_locus_tag="pgaptmp_003660"
     CDS             <1..519
                     /gene="lpfA"
                     /locus_tag="ACHOSC_RS18300"
                     /old_locus_tag="pgaptmp_003660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000756357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long polar fimbrial major subunit LpfA"
                     /protein_id="HGU4549519.1"
                     /translation="KVLFALSALALTSTSVFAADAGDGTVKFTGEIVDAACVVSPDTQ
                     KQEVVLGQVNKSVFTAMGDKSTATPFKIKLENCDISTFKNVEISFNGVGDADNSKLVS
                     VSTEPGAATGVGIGIYDNTNTLVDLNTGKSATVLKEGQTVLYFTANYVSTKDAVTTGY
                     GNAEVDFNLTYN"
     gene            603..1289
                     /gene="lpfB"
                     /locus_tag="ACHOSC_RS18305"
                     /old_locus_tag="pgaptmp_003661"
     CDS             603..1289
                     /gene="lpfB"
                     /locus_tag="ACHOSC_RS18305"
                     /old_locus_tag="pgaptmp_003661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000822474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long polar fimbrial biogenesis chaperone LpfB"
                     /protein_id="HGU4549520.1"
                     /translation="MKRISFAVLALLMTAQQCFAGGVALSTTRVIYDGGKKEASLTVE
                     NHNKNEEFLIQSWIDDVNGNKKTPFIITPPLFKLDPDKNNVLRIVNIEDTLPKDRESV
                     YWINVKAIPAKSAENENKNVLQIAVRTRLKLFYRPKGLPGSNLEAMKQLRFQRQNNQI
                     VVNNDSAYNLTFNQFSINGQKIERAGMVLAKGKLNINLPAGTGNAHEVKYSIINDFGV
                     AGEMLTKPIN"
     gene            1313..3844
                     /gene="lpfC"
                     /locus_tag="ACHOSC_RS18310"
                     /old_locus_tag="pgaptmp_003662"
     CDS             1313..3844
                     /gene="lpfC"
                     /locus_tag="ACHOSC_RS18310"
                     /old_locus_tag="pgaptmp_003662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001759518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane usher protein LpfC"
                     /protein_id="HGU4549521.1"
                     /translation="MHWMHLPLYHYRAHFSFSLLALTIASSLPAYGGKFNPKFLENVQ
                     GIDQHIDLSVYDSPVGQQIPGKYRVSVFVNEEKMASRTLDFSTASEAKRKASGESLMP
                     CLSRVQLEEMGVRVDSFPALKMSPPEACVAFDEIIPQATSRFDFNTQTLHLSFPQAAM
                     MMTARGTVDPSRWDEGIPALLLDYSFSGSNGRNEGTGSSSDSTSDSYYLNLRSGLNVG
                     PWRLRNNSIWNRTDGKNQWDNVGTSLNRAIIPLKSQITLGDTATPGEIFDSVQMRGAL
                     LASDDEMLPDSQRGFAPVVRGIAKSNAEVSIEQNGYVIYRTFVQPGAFEINDLYATSG
                     SGDLTVIIKEADGSEQRFIQPFSAVAIFQREGYLKYSLAAGEYRAGNYDSDKPHFGQF
                     TAMYGLPWGMTAYGGALLSADYNALALGLGKNFGTIGAVSVDVTQAKSQLRNNEKEEG
                     QSYRFLYSKSFEGGTDLRLLGYKYSTSGYYTFQEATDVRSDADSDYRRYHKRSQIQGN
                     ITQQLGDYGSVYFNMTQQDYWNVDGKENSLSAGYHGHIGRVNYSVAYTWTRSPEWEED
                     DRLWSFSVSIPLGGAWSSYRMTTDQNGKTSQQASVSGTLLEDRNLSYNVQQGYTSNGV
                     GYSGSVNMGYMGGSGNIDVGYNYSKDNQQVNYGVRGGVIVHSEGITLSQPLGESLAIV
                     SAPGARGGHVVNSSGVEVDWMGNAVVPYLTPYRETIVELRSDTLGQNVELQEAFQKVV
                     PTRGAVVRSRFDTRVGYRVLMSLKQANGNAVPFGATAALIDESKPASSIVGEEGQLYI
                     SGMPEEGELQVSWGNEQAQRCRVPFRLPENKDNAAIVMVNAVCEK"
     gene            3855..4910
                     /gene="lpfD"
                     /locus_tag="ACHOSC_RS18315"
                     /old_locus_tag="pgaptmp_003663"
     CDS             3855..4910
                     /gene="lpfD"
                     /locus_tag="ACHOSC_RS18315"
                     /old_locus_tag="pgaptmp_003663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000694923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long polar fimbrial protein LpfD"
                     /protein_id="HGU4549522.1"
                     /translation="MKIKNLLAGMVLLGTSTFAGNIWAADWGPCQTSNGVAHEYSFDF
                     VQTIQVPSENKAGKILTQPFALGTKYSAYCECPDPIPDNGVVTYFKGVTLLPEPGTVD
                     GYYKFNNYIDILTKIYVYTQLDIPVPFTDRSNGTAQKECTPYTANNWGTGGKGSISIY
                     ISHPFVGQMIIPKTRVASLFGTKKKGVYNDSQPMANVSISGSITVTQGCELAAGTVLD
                     IPFGEYQAHDFKGRAGQPPQNVQKVQKELSFDCNNISDGVKIYLSIDATPNTAYPSAI
                     DLGNADVGAVIEDGKGNILKPNDSNSLLEMNPGSLYEDVKRKATTTITAYPVSTTGKL
                     PAAGDYSGIATIHVELE"
     gene            4916..5440
                     /gene="lpfE"
                     /locus_tag="ACHOSC_RS18320"
                     /old_locus_tag="pgaptmp_003664"
     CDS             4916..5440
                     /gene="lpfE"
                     /locus_tag="ACHOSC_RS18320"
                     /old_locus_tag="pgaptmp_003664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000831418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long polar fimbrial protein LpfE"
                     /protein_id="HGU4549523.1"
                     /translation="MKRTILALCLLPLSFSSFAATTDLGPKGTLKFTLKISQGACELV
                     KDSVEVDMGTAVLKKPVRIGTEINPTPFSIGLKNCSEVVRAYVTMDGTPNADDSNLFA
                     LDAGGATGVGLKIKTAAGVQQFPENTTPTPVEFAINGDGAHQLNYIASYVPIRADATI
                     GRADATVDFTVQYE"
     gene            5693..7384
                     /gene="eptB"
                     /locus_tag="ACHOSC_RS18325"
                     /old_locus_tag="pgaptmp_003665"
     CDS             5693..7384
                     /gene="eptB"
                     /locus_tag="ACHOSC_RS18325"
                     /old_locus_tag="pgaptmp_003665"
                     /EC_number="2.7.8.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418002.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="kdo(2)-lipid A phosphoethanolamine
                     7''-transferase"
                     /protein_id="HGU4549524.1"
                     /translation="MRYIKSITQQKLSFLLAIYIGLFMNGAVFYRRFGSYAHDFTVWK
                     GVSAVVELAATVLVTFFLLRLLSLFGRRSWRILASLVVLFSAGASYYMTFLNVVIGYG
                     IIASVMTTDIDLSKEVVGLNFILWLIAVSALPLILIWNNRCRYTLLRQLRTPGQRIRS
                     LAVVVLAGIMVWAPIRLLDIQQKKVERATGVDLPSYGGVVANSYLPSNWLSALGLYAW
                     ARVDESSDNNSLLNPAKKFTYQAPQNVDDTYVVFIIGETTRWDHMGIFGYERNTTPKL
                     AQEKNLAAFRGYSCDTATKLSLRCMFVRQGGAEDNPQRTLKEQNIFAVLKQLGFSSDL
                     YAMQSEMWFYSNTMADNIAYREQIGAEPRNRGKPVDDMLLVDEMQQSLGRNPDGKHLI
                     ILHTKGSHFNYTQRYPRSFAQWKPECIGVDSGCTKAQMINSYDNSVTYVDHFISSVID
                     QVRDKKAIVFYAADHGESINEREHLHGTPRELAPPEQFRVPMMVWMSDKYLENPANAQ
                     AFAQLKKEADMKVPRRHVELYDTIMGCLGYTSPDGGINENNNWCHIPQTKVAAAN"
     gene            7476..7552
                     /locus_tag="ACHOSC_RS18330"
                     /old_locus_tag="pgaptmp_003666"
     tRNA            7476..7552
                     /locus_tag="ACHOSC_RS18330"
                     /old_locus_tag="pgaptmp_003666"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:7510..7512,aa:Pro,seq:cgg)
     gene            7587..7720
                     /locus_tag="ACHOSC_RS18335"
                     /old_locus_tag="pgaptmp_003667"
     ncRNA           7587..7720
                     /ncRNA_class="other"
                     /locus_tag="ACHOSC_RS18335"
                     /old_locus_tag="pgaptmp_003667"
                     /product="RtT sRNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00391"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="rtT sRNA, processed from tyrT transcript; Derived
                     by automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF00391"
BASE COUNT         2024 a         1803 c         1984 g         1996 t
ORIGIN      
        1 aaggttctgt ttgccttatc tgcgcttgcg ctgacctcta cttccgtatt cgcagctgat
       61 gcgggcgacg gtactgttaa attcaccggt gagattgttg acgccgcttg tgttgtgtct
      121 ccagacactc aaaaacaaga agttgttctg ggtcaggtga acaagtctgt atttactgct
      181 atgggtgata aatccacagc aacgccgttc aaaatcaaac tggaaaactg cgatatctcc
      241 actttcaaaa atgtggaaat cagctttaac ggcgttggcg atgcagacaa cagcaaactg
      301 gtttctgtaa gcactgaacc aggtgccgca actggcgtag gtatcggtat ttatgataac
      361 accaataccc tggttgacct gaatactggt aaatctgcta ctgtcctgaa agaaggtcag
      421 accgttctgt atttcaccgc taactatgtc tctaccaaag atgcagtaac cacgggttac
      481 ggtaacgctg aagtcgactt caacctgact tacaactaat cgatcctgac ggaacgatca
      541 tctctcaatg gcaacagaga ctgtgttgcc attattcaca ccaatgactc agggaccgaa
      601 tcatgaaacg tattagcttt gctgtgctgg cacttttaat gaccgcacaa cagtgttttg
      661 ctggtggcgt agccttaagc acgacgcgtg ttatttatga tggcggcaag aaagaagcgt
      721 cgctgaccgt tgaaaaccat aataaaaatg aagagttttt gatccagtca tggattgatg
      781 atgtgaatgg caataaaaaa acaccgttta ttattacacc gccattattc aaacttgatc
      841 ctgataaaaa taacgtctta cgtatcgtca atattgaaga taccttacct aaagatcgtg
      901 aatccgttta ctggattaat gtaaaggcca ttcctgcgaa aagtgctgaa aatgaaaata
      961 aaaacgtctt acagatagcc gttcgcaccc gtttgaagtt gttctatcgt ccaaaaggtt
     1021 tgccgggaag caacctcgag gccatgaaac agttacgttt ccagcgtcaa aataaccaaa
     1081 ttgtggttaa taacgactcc gcttataacc tgacctttaa tcagttttcc attaatggac
     1141 aaaagattga acgggcggga atggttctcg cgaaaggaaa gttaaacatt aaccttccag
     1201 ccggtacagg caacgcgcac gaagtgaaat acagcattat taatgatttc ggtgttgctg
     1261 gcgaaatgct gacgaagccg attaactgat ccttttacaa ggcgctttta ctatgcattg
     1321 gatgcattta cctctctatc actacagagc gcatttttcc ttctctctgc tggcattaac
     1381 gattgccagc tcgttacctg cgtatggcgg aaaatttaac cctaaatttc tcgaaaacgt
     1441 tcagggtatc gatcaacaca tcgacttgtc ggtatacgac tcacctgtcg gccagcaaat
     1501 ccccggtaaa taccgggttt cggtgtttgt aaacgaagaa aaaatggctt cccggacgct
     1561 ggattttagt accgccagcg aagcgaaacg caaagccagc ggtgagtcgc ttatgccttg
     1621 ccttagccgg gtgcaactgg aagaaatggg cgtgcgcgtt gacagctttc cggcgctgaa
     1681 gatgtcacca ccagaagcct gcgtagcgtt tgatgaaatc attccccagg ccaccagccg
     1741 ctttgatttt aatactcaga cgctgcatct ttcctttcca caggcggcaa tgatgatgac
     1801 cgctcgcggc acggttgatc cttctcgctg ggacgaagga atacctgcgc tgttgctgga
     1861 ttacagcttt agtggcagca atggacgcaa tgaaggtaca ggttcatctt cagattcgac
     1921 cagtgacagt tattacctga acctgcgcag cggtttgaac gttggtccgt ggcgtttacg
     1981 taataacagt atctggaacc gtaccgatgg caaaaaccaa tgggataacg ttggtacatc
     2041 gttaaaccgg gcaattattc cacttaaatc acagataact ctgggtgata ccgccacgcc
     2101 tggcgagatt tttgatagcg tacaaatgcg tggggcgtta ctggcatccg acgatgaaat
     2161 gttgccggac agccagcgtg gctttgctcc ggtagtgcgc ggcatcgcca aaagcaacgc
     2221 tgaagtgagt atcgaacaga acggctatgt catttatcgc acttttgttc agccgggtgc
     2281 atttgagatt aacgatctct acgcaacctc cggcagcggc gacctgacgg tgatcattaa
     2341 agaagccgac ggaagtgaac aacgttttat ccagccgttt tcggcggtgg cgattttcca
     2401 gcgtgaaggg tatctgaaat acagtctggc tgccggtgaa tatcgtgctg gtaattacga
     2461 cagcgataaa ccacatttcg ggcagtttac cgccatgtac ggcttgccgt ggggtatgac
     2521 cgcctacggt ggtgcattgt tgtcggcaga ttacaacgct ttagctctgg gcctggggaa
     2581 aaacttcggt accattggcg cagtatcggt ggatgtcact caggcgaaaa gtcagttacg
     2641 caacaatgaa aaagaggaag gccagtctta tcgcttcctc tattcgaaaa gttttgaggg
     2701 tggcactgac ctgcgtctgt tgggctacaa atactcaacc agcggctact acaccttcca
     2761 ggaagcaact gatgttcgca gtgacgccga cagtgattac cgccgttatc acaagcgcag
     2821 ccagatacag ggcaatatta cccagcaact gggagactac ggctcggttt atttcaatat
     2881 gacgcagcag gattactgga acgttgacgg taaggaaaac tccttaagcg ccggttatca
     2941 cggtcacatt ggtcgggtga attacagcgt ggcctatacc tggacgagaa gtccggaatg
     3001 ggaagaagac gatcgcttgt ggtcattcag tgtttccatc cctctggggg gcgcgtggag
     3061 cagttatcgc atgactaccg accagaatgg caagacgtcg caacaggcct ctgttagcgg
     3121 cacgttactg gaagatcgta acctgagcta taacgttcag cagggttaca ccagtaatgg
     3181 cgtaggctac agtggcagcg tcaacatggg gtatatgggt ggctctggta acattgacgt
     3241 aggctataac tattctaaag ataaccaaca ggttaactat ggcgtgcgcg gcggtgtcat
     3301 cgttcacagt gaaggtataa cgctttcgca gcctttaggc gaaagtctgg cgattgtttc
     3361 tgcaccgggc gcgcgtggtg gacatgtggt taacagcagc ggcgtagaag ttgactggat
     3421 gggcaacgcc gtggtgcctt atctcacacc atatcgtgaa accattgtgg aattgcgtag
     3481 cgacacgttg gggcaaaacg ttgaactaca ggaagcgttc cagaaagtcg tgcctactcg
     3541 tggtgcagtt gtgcgttccc gctttgatac ccgcgtcggt taccgtgtgc tgatgagctt
     3601 aaaacaggcg aatggcaacg cagtaccgtt tggtgcaacc gcagcgttaa ttgatgagag
     3661 caaacctgcc agcagcattg tgggtgaaga aggacagctt tatatcagcg ggatgccaga
     3721 agagggcgaa ttgcaggtga gctgggggaa cgagcaggcg caacgttgcc gtgtgccatt
     3781 ccgcttgcca gagaacaagg acaatgcggc tatcgtgatg gttaatgcgg tatgcgagaa
     3841 ataaggatat ttagatgaaa attaaaaatt tgttggcagg tatggtgctg ttagggacga
     3901 gcacttttgc tggaaatata tgggctgcgg attggggacc atgtcagaca tcaaatgggg
     3961 tggcccatga gtatagtttt gatttcgtac agactatcca ggtaccttca gaaaataagg
     4021 ctgggaaaat attaactcag ccctttgctt taggtacaaa atattccgct tattgtgaat
     4081 gcccggatcc cattccagat aatggagttg tgacttattt taaaggtgta acgcttctgc
     4141 ctgagcctgg aactgtagat gggtactata aattcaataa ttacattgat attctcacca
     4201 aaatatatgt ctacacacaa ttagatatcc cggtgccttt taccgatcga agcaatggca
     4261 cggcacagaa agaatgtacg ccctatactg ctaacaattg ggggacaggt ggtaaaggaa
     4321 gtatcagtat ttatatttca catccttttg ttgggcaaat gattattcct aaaacacgag
     4381 ttgcgtccct ctttgggaca aaaaagaaag gtgtttacaa tgatagtcag cccatggcca
     4441 atgtcagtat ttccgggagt attacagtta ctcaaggctg tgaacttgcc gctggcaccg
     4501 tactggatat tccattcgga gaatatcagg cccatgattt taaagggcgt gccggacaac
     4561 caccacaaaa tgttcagaag gtacaaaaag aattaagctt tgactgcaat aatatttcag
     4621 atggcgtcaa aatttatctc agtattgatg caacaccaaa tactgcctat cccagcgcaa
     4681 ttgatttagg caacgccgat gttggtgcag tcattgagga cggtaaaggt aatattctta
     4741 agccgaatga cagtaatagc ttattagaaa tgaacccagg ctcgctgtat gaagatgtca
     4801 aacgcaaagc gacaaccaca attaccgctt atcccgttag cactaccgga aaattacccg
     4861 ctgccgggga ttatagcggt attgctacca tacatgtcga actggagtaa cagtcatgaa
     4921 gcgaacaata cttgcgttat gcctgctgcc tctttccttt tcgtcattcg cggcaaccac
     4981 ggatttaggg ccgaaaggaa cattgaaatt cacccttaaa atcagccagg gtgcttgcga
     5041 actggtgaaa gacagcgttg aggttgacat gggcaccgcg gttctcaaaa agccggtaag
     5101 aatcggaacg gaaattaatc ccactccttt ttccatcggc ctgaaaaact gtagcgaagt
     5161 ggtcagagcc tacgtgacta tggacggtac acccaatgca gacgactcaa atctttttgc
     5221 gctggatgcg ggtggcgcta caggcgttgg gctgaaaatc aaaaccgctg ctggagtaca
     5281 acagttcccg gaaaatacaa cgccgacacc ggttgagttt gcaattaacg gtgatggcgc
     5341 acatcaactg aactatatcg ccagctacgt gcctattcgc gccgatgcaa ctattggtcg
     5401 cgcagatgca acggtcgact ttactgtaca gtacgaataa tttcacatct taagggccag
     5461 ttaactggcc ctttttactg ctttttgtaa cgctaaagtc tcttttcaaa cttgcatttt
     5521 tgtaaatttg tgcttcatgc acactctttc cccacacttt ttcgctttgc tgtggtctac
     5581 ttattcgcgc gtgtagattt tacttatctg actacctccg cacttttccc ctgccgggcc
     5641 tgaaaagccg ctaagcaggg tgttatcacc tgtttgtcca gggtttgttt gcatgagata
     5701 catcaaatcg attacacagc agaagctgag ctttttgctt gcaatctata ttggcctttt
     5761 tatgaatggc gcggtttttt atcgccgctt cggcagctat gcgcacgatt ttaccgtctg
     5821 gaaaggcgtt tctgctgttg ttgaactggc cgccaccgta ctggtgacct tctttttact
     5881 acgtcttcta tcgctgtttg gccgccgcag ctggcgtatt ctggcgtcgc tggtggtgct
     5941 cttttccgca ggtgccagct attacatgac cttccttaat gtggtcattg gttatggcat
     6001 catcgcttcc gtcatgacca ccgatatcga cctgtcaaaa gaagttgttg gtctgaactt
     6061 tattctctgg ttaatcgccg ttagtgcatt gcctcttatc cttatctgga ataaccgctg
     6121 tcgctacacc ttgctccgac aactgcgaac cccagggcag cgtattcgca gcctggcggt
     6181 cgtcgtactg gcgggtatta tggtttgggc accgattcgt ttgctggata tccagcagaa
     6241 gaaagtggag agggcgaccg gcgttgattt gccgagttat ggcggtgtcg tagcgaactc
     6301 ttatctgcca tcaaactggc tttctgcgtt ggggctgtat gcctgggcgc gggtcgatga
     6361 atcttccgat aataattcat tgcttaatcc ggcgaagaaa ttcacttatc aggcaccgca
     6421 aaacgttgat gacacttatg tcgtgtttat catcggtgaa accacgcgtt gggaccatat
     6481 gggcattttc ggctatgagc gtaataccac gccgaaactg gcccaggaga aaaatctggc
     6541 ggcgttccgt ggttactcct gtgataccgc aaccaaactc tcactgcgtt gcatgtttgt
     6601 acgtcagggg ggcgcggaag ataatccgca gcgcacatta aaagaacaga acattttcgc
     6661 ggttctgaag cagttaggat tcagttctga cctctacgct atgcagagcg aaatgtggtt
     6721 ctacagcaac acgatggcgg acaacattgc ttatcgtgag cagattggtg cggagccacg
     6781 taatcgtggc aagccggtag atgatatgtt gctggtagac gaaatgcagc aatcgctagg
     6841 gcgcaacccg gatggtaagc atctgatcat tctgcatacc aaaggttcgc attttaacta
     6901 cacccagcgt tatccgcgta gcttcgcgca gtggaagccg gaatgtattg gtgttgatag
     6961 cggctgtacc aaagcgcaga tgatcaactc ctatgacaac tcggtgacct atgtggatca
     7021 ctttatctcc agcgtgattg atcaggttcg cgataagaaa gcgattgtgt tctacgcagc
     7081 tgaccacggt gagtcaatta atgaacgcga gcacctgcac ggcacgccgc gtgaactggc
     7141 accgccggag cagttccgcg taccgatgat ggtctggatg tcagataaat atctggaaaa
     7201 tccggccaat gctcaggcat ttgcgcagct gaaaaaagaa gccgacatga aagtgccacg
     7261 ccgtcatgta gagctgtacg acaccatcat gggttgtctt ggctatactt caccggatgg
     7321 tggaattaac gaaaacaaca actggtgtca catcccgcag acaaaagtgg cagcggctaa
     7381 ctaagcggcc tgctgacttt ctcgccgatc aaaaggcatt ttgctattaa gggattgacg
     7441 agggcgtatc tgcgcagtaa gatgcgcccc gcattcggtg attggcgcag cctggtagcg
     7501 cacttcgttc gggacgaagg ggtcggaggt tcgaatcctc tatcaccgac caaattcgaa
     7561 aagcctgctc agcgagcagg cttttttgca tttgaagccc atgaggatga gaacctccgg
     7621 gggcggggag gttcgactcg agcgaagcga gagaacgttg cgccagcaac ggcccgcagg
     7681 gcgagccacg aagtggcgag taatcctcta tcaccgacca aattcgaaaa gcctgctcaa
     7741 cgagcaggct tttttgcatt tgtagctcat gaggatgaga acctccgggg gcggggaggt
     7801 tcgactc
//
LOCUS       DAZKHF020000206         7562 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2201S31241
            SAMN43267800-rid25106413.denovo.208, whole genome shotgun sequence.
ACCESSION   DAZKHF020000206 DAZKHF020000000
VERSION     DAZKHF020000206.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267800
            Sequence Read Archive: SRR30307263
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 7562)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7562)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 7562)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350581.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350581.2
            Genome Coverage       :: 151x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:21:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,652
            CDSs (total)                      :: 5,557
            Genes (coding)                    :: 5,320
            CDSs (with protein)               :: 5,320
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 237
            CDSs (without protein)            :: 237
            Pseudo Genes (ambiguous residues) :: 0 of 237
            Pseudo Genes (frameshifted)       :: 102 of 237
            Pseudo Genes (incomplete)         :: 130 of 237
            Pseudo Genes (internal stop)      :: 52 of 237
            Pseudo Genes (multiple problems)  :: 41 of 237
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7562
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267800-rid25106413.denovo.208"
                     /serotype="O 26:H11"
                     /isolate="2201S31241"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-01"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            112..1509
                     /gene="ulaA"
                     /locus_tag="ACHOSC_RS18340"
                     /old_locus_tag="pgaptmp_003668"
     CDS             112..1509
                     /gene="ulaA"
                     /locus_tag="ACHOSC_RS18340"
                     /old_locus_tag="pgaptmp_003668"
                     /EC_number="2.7.1.194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418614.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS ascorbate transporter subunit IIC"
                     /protein_id="HGU4549525.1"
                     /translation="MEILYNIFTVFFNQVMTNAPLLLGIVTCLGYILLRKSVSVIIKG
                     TIKTIIGFMLLQAGSGILTSTFKPVVAKMSEVYGINGAISDTYASMMATIDRMGDAYS
                     WVGYAVLLALALNICYVLLRRITGIRTIMLTGYIMFQQAGLIAVTLFIFGYSMWTTII
                     CTAILVSLYWGITSNMMYKPTQEVTDGCGFSIGHQQQFASWIAYKVAPFLGKKEESVE
                     DLKLPGWLNIFHDNIVSTAIVMTIFFGAILLSFGIDTVQAMAGKVNWTVYILQTGFSF
                     AVAIFIITQGVRMFVAELSEAFNGISQRLIPGAVLAIDCAAIYSFAPNAVVWGFMWGT
                     IGQLIAVGILVACGSSILIIPGFIPMFFSNATIGVFANHFGGWRAALKICLVMGMIEI
                     FGCVWAVKLTGMSAWMGMADWSILAPPMMQGFFSIGIAFMAVIIVIALAYMFFAGRAL
                     RAEEDAEKQLAEQSA"
     gene            1525..1830
                     /gene="ulaB"
                     /locus_tag="ACHOSC_RS18345"
                     /old_locus_tag="pgaptmp_003669"
     CDS             1525..1830
                     /gene="ulaB"
                     /locus_tag="ACHOSC_RS18345"
                     /old_locus_tag="pgaptmp_003669"
                     /EC_number="2.7.1.194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710059.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS ascorbate transporter subunit IIB"
                     /protein_id="HGU4549526.1"
                     /translation="MTVRILAVCGNGQGSSMIMKMKVDQFLTQSNIDHTVNSCAVGEY
                     KSELSGADIIIASTHIAGEITVTGNKYVVGVRNMLSPADFGPKLLEVIKAHFPQDVK"
     gene            1840..2304
                     /gene="ulaC"
                     /locus_tag="ACHOSC_RS18350"
                     /old_locus_tag="pgaptmp_003670"
     CDS             1840..2304
                     /gene="ulaC"
                     /locus_tag="ACHOSC_RS18350"
                     /old_locus_tag="pgaptmp_003670"
                     /EC_number="2.7.1.194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS ascorbate transporter subunit IIA"
                     /protein_id="HGU4549527.1"
                     /translation="MKLRDSLAENKSIRLQAEAETWQDAVKIGVDLLVAADVVEPRYY
                     QAILDAVEQHGPYFVLAPGLAMPHGRPEEGVKKTGFALVTLKKPLEFNHEDNDPVDIL
                     ITMAAVDANTHQEVGIMQIVNLFEDEENFDRLRACRTEQEVLDLIDRTNAAA"
     gene            2318..2968
                     /gene="ulaD"
                     /locus_tag="ACHOSC_RS18355"
                     /old_locus_tag="pgaptmp_003671"
     CDS             2318..2968
                     /gene="ulaD"
                     /locus_tag="ACHOSC_RS18355"
                     /old_locus_tag="pgaptmp_003671"
                     /EC_number="4.1.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-keto-L-gulonate-6-phosphate decarboxylase
                     UlaD"
                     /protein_id="HGU4549528.1"
                     /translation="MSLPMLQVALDNQTMDSAYETTRLIAEEVDIIEVGTILCVGEGV
                     RAVRDLKALYPHKIVLADAKIADAGKILSRMCFEANADWVTVICCADINTAKGALDVA
                     KEFNGDVQIELTGYWTWEQAQQWRDAGIQQVVYHRSRDAQAAGVAWGEADITAIKRLS
                     DMGFKVTVTGGLALEDLPLFKGIPIHVFIAGRSIRDAASPVEAARQFKRSIAELWG"
     gene            2978..3832
                     /gene="ulaE"
                     /locus_tag="ACHOSC_RS18360"
                     /old_locus_tag="pgaptmp_003672"
     CDS             2978..3832
                     /gene="ulaE"
                     /locus_tag="ACHOSC_RS18360"
                     /old_locus_tag="pgaptmp_003672"
                     /EC_number="5.1.3.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 3-epimerase UlaE"
                     /protein_id="HGU4549529.1"
                     /translation="MLSKQIPLGIYEKALPAGECWLERLQLAKTLGFDFVEMSVDETD
                     ERLSRLDWSREQRLALVNAIVETGVRVPSMCLSAHRRFPLGSEDDAVRAQGLEIMRKA
                     IQFAQDVGIRVIQLAGYDVYYQEANNETRRRFRDGLKESVEMASRAQVTLAMEIMDYP
                     LMNSISKALGYAHYLNNPWFQLYPDIGNLSAWDNDVQMELQAGIGHIVAVHVKDTKPG
                     VFKNVPFGEGVVDFERCFETLKQSGYCGPYLIEMWSETAEDPAAEVAKARDWVKARMA
                     KAGMVEAA"
     gene            3832..4518
                     /gene="ulaF"
                     /locus_tag="ACHOSC_RS18365"
                     /old_locus_tag="pgaptmp_003673"
     CDS             3832..4518
                     /gene="ulaF"
                     /locus_tag="ACHOSC_RS18365"
                     /old_locus_tag="pgaptmp_003673"
                     /EC_number="5.1.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 4-epimerase UlaF"
                     /protein_id="HGU4549530.1"
                     /translation="MQKLKQQVFEANMDLPRYGLVTFTWGNVSAIDRERGLVVIKPSG
                     VAYETMKADDMVVVDMSGKVVEGEYRPSSDTATHLELYRRYPSLGGIVHTHSTHATAW
                     AQAGLAIPALGTTHADYFFGDIPCTRGLSEEEVQGEYELNTGKVIIETLGNAEPLHTP
                     GIVVYQHGPFAWGKDAHDAVHNAVVMEEVAKMAWIARSINPQLNHIDSFLMNKHFMRK
                     HGPNAYYGQK"
     gene            complement(4647..4922)
                     /gene="yjfY"
                     /locus_tag="ACHOSC_RS18370"
                     /old_locus_tag="pgaptmp_003674"
     CDS             complement(4647..4922)
                     /gene="yjfY"
                     /locus_tag="ACHOSC_RS18370"
                     /old_locus_tag="pgaptmp_003674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1471 family protein YjfY"
                     /protein_id="HGU4549531.1"
                     /translation="MFSRVLALLAVLLLSANTWAAIEINNHQARNMDDVQSLGVIYIN
                     HNFATESEARQALNEETDAQGATYYHVILMREPGSNGNMHASADIYR"
     gene            5249..5644
                     /gene="rpsF"
                     /locus_tag="ACHOSC_RS18375"
                     /old_locus_tag="pgaptmp_003675"
     CDS             5249..5644
                     /gene="rpsF"
                     /locus_tag="ACHOSC_RS18375"
                     /old_locus_tag="pgaptmp_003675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020456251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S6"
                     /protein_id="HGU4549532.1"
                     /translation="MRHYEIVFMVHPDQSEQVPGMIERYTAAITGAEGKIHRLEDWGR
                     RQLAYPINKLHKAHYVLMNVEAPQEVIDELETTFRFNDAVIRSMVMRTKHAVTEASPM
                     VKAKDERRERRDDFANETADDAEAGDSEE"
     gene            5651..5965
                     /gene="priB"
                     /locus_tag="ACHOSC_RS18380"
                     /old_locus_tag="pgaptmp_003676"
     CDS             5651..5965
                     /gene="priB"
                     /locus_tag="ACHOSC_RS18380"
                     /old_locus_tag="pgaptmp_003676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030894 - replisome [Evidence IEA];
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal replication protein N"
                     /protein_id="HGU4549533.1"
                     /translation="MTNRLVLSGTVCRTPLRKVSPSGIPHCQFVLEHRSVQEEAGFHR
                     QAWCQMPVIVSGHENQAITHSITVGSRITVQGFISCHKAKNGLSKMVLHAEQIELIDS
                     GD"
     gene            5970..6197
                     /gene="rpsR"
                     /locus_tag="ACHOSC_RS18385"
                     /old_locus_tag="pgaptmp_003677"
     CDS             5970..6197
                     /gene="rpsR"
                     /locus_tag="ACHOSC_RS18385"
                     /old_locus_tag="pgaptmp_003677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012986941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S18"
                     /protein_id="HGU4549534.1"
                     /translation="MARYFRRRKFCRFTAEGVQEIDYKDIATLKNYITESGKIVPSRI
                     TGTRAKYQRQLARAIKRARYLSLLPYTDRHQ"
     gene            6239..6688
                     /gene="rplI"
                     /locus_tag="ACHOSC_RS18390"
                     /old_locus_tag="pgaptmp_003678"
     CDS             6239..6688
                     /gene="rplI"
                     /locus_tag="ACHOSC_RS18390"
                     /old_locus_tag="pgaptmp_003678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005134442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L9"
                     /protein_id="HGU4549535.1"
                     /translation="MQVILLDKVANLGSLGDQVNVKAGYARNFLVPQGKAVPATKKNI
                     EFFEARRAELEAKLAEVLAAANARAEKINALETVTIASKAGDEGKLFGSIGTRDIADA
                     VTAAGVEVAKSEVRLPNGVLRTTGEHEVSFQVHSEVFAKVIVNVVAE"
     gene            complement(6759..7553)
                     /gene="yjfZ"
                     /locus_tag="ACHOSC_RS18395"
                     /old_locus_tag="pgaptmp_003679"
     CDS             complement(6759..7553)
                     /gene="yjfZ"
                     /locus_tag="ACHOSC_RS18395"
                     /old_locus_tag="pgaptmp_003679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2686 family protein"
                     /protein_id="HGU4549536.1"
                     /translation="MTLPTTIYSFPAYSPHFSSTDKPVKLKFHQYARATLLPNRGRDH
                     NCDGRRTVEIHKLDLSDWQAFNKLATKCNAYDGVTMNGDNSFGWNHKATLDNIHAQKY
                     NKAHAGARLTAELKYLLQDVESFEPNSKYTIHEVVLGPGYGTPDYTGQTIGYVVTLPA
                     QMPNCWSSELPTIDLYIDQLRTVTGVSNALGFIIAALLNAYSDLPHDLKIGLRSLSNS
                     AAIYSGLGFERVPQDRDIRSDRMHLTPANHPDLWTQENGEWIYLRN"
BASE COUNT         1812 a         1828 c         2064 g         1858 t
ORIGIN      
        1 gtcacattta atcataaata atcttgttgt gattactttt gaaaattaga gtgagtgcac
       61 aacattccgg gtgtgcggaa tacccggtta cctcttcttc aggagatcgt tatggagatc
      121 ctctacaaca tctttaccgt gttttttaac caggtcatga ccaatgcccc gttgttgctg
      181 ggtattgtga cctgtctggg ctacatccta ctgcgcaaaa gtgtcagcgt tattattaaa
      241 ggcacgatta aaaccataat tggtttcatg ttgttgcagg cagggtccgg catcctcacc
      301 agcaccttca aaccggtggt ggcgaaaatg tccgaagtct acggcattaa cggcgcaatt
      361 tccgatactt acgcgtcgat gatggcaacc atcgaccgca tgggcgatgc ctatagctgg
      421 gtaggttacg cggtgttgtt agcgctggcg ctgaacatct gttacgtgct gctgcgtcgc
      481 attaccggta ttcgtaccat catgctgact ggttatatca tgttccagca ggcggggctg
      541 attgccgtta cgttgtttat tttcggctac tccatgtgga caaccattat ctgcacggcg
      601 attctggttt cgctctactg gggcattacc tccaacatga tgtacaagcc gactcaggaa
      661 gtgacggacg gctgcggttt ctccatcggt caccagcaac agtttgcatc atggattgcc
      721 tataaagttg cgccgttcct cggcaaaaaa gaggaaagcg ttgaagacct caaactgcca
      781 ggctggctga atattttcca cgacaacatc gtctccacgg cgattgtgat gaccatcttc
      841 tttggtgcca ttctgctctc cttcggtatc gacaccgtgc aggcgatggc aggcaaagtg
      901 aactggacgg tgtacatcct gcaaactggt ttctccttcg cggtggcgat cttcatcatc
      961 actcagggcg tgcgcatgtt tgtggcggaa ctctctgaag catttaacgg tatctcccag
     1021 cgcctgatcc ctggtgcggt tctggcgatt gactgtgcgg ctatctatag cttcgcgccg
     1081 aacgccgtgg tttggggctt tatgtggggc accatcggtc agctgattgc ggttggcatc
     1141 ctggtcgcct gcggttcctc gatcctgatt attcccggct ttatcccgat gttcttctcc
     1201 aacgccacca tcggcgtgtt cgctaaccac ttcggcggct ggcgtgcggc gctgaagata
     1261 tgtctggtga tggggatgat tgaaatcttt ggctgcgtct gggcggtgaa actcaccggt
     1321 atgagcgcct ggatgggcat ggcggactgg tcgattctgg caccgccgat gatgcagggc
     1381 ttcttctcca tcggtatcgc ctttatggcc gtcatcattg taattgcact ggcttatatg
     1441 ttcttcgctg gccgcgcact gcgcgcagaa gaagatgcag aaaaacaact ggcagaacag
     1501 tctgcttaat aaggagtttt gattatgacc gtacgtattc tggctgtgtg tggcaacgga
     1561 caaggcagtt ccatgatcat gaagatgaaa gtggaccagt ttttaaccca atcaaacatt
     1621 gaccacacgg taaatagctg cgcggttggc gaatacaaaa gcgagttgag cggcgcggat
     1681 atcatcatcg cttctacgca cattgcgggc gaaatcaccg tgaccggcaa caaatacgtg
     1741 gttggcgtgc gcaacatgct ctctcctgcc gactttggcc cgaaactgct ggaagtgatc
     1801 aaagcgcatt tcccgcagga tgtgaagtaa ggacgcgcca tgaaattacg tgattcgctg
     1861 gcggagaata aatccatccg cctgcaggct gaagctgaga catggcagga cgcagtaaaa
     1921 atcggcgttg acctgctggt ggcggcggat gttgtcgagc cgcgttacta ccaggcgatt
     1981 ctggatgcgg ttgaacagca cggtccgtac tttgtgctcg caccaggcct ggcaatgccg
     2041 cacggtcgcc cggaagaggg cgttaagaaa accggttttg cgctggtgac gctgaaaaag
     2101 ccgctggagt tcaaccacga agataacgac ccggtggata tcctcatcac catggcggcg
     2161 gtcgatgcca acacgcacca ggaagtgggc atcatgcaga tcgtcaacct gtttgaagat
     2221 gaagaaaatt tcgaccgttt acgtgcctgc cgtaccgagc aggaagtact ggatctcatt
     2281 gaccgcacca acgcggcagc ttaagaagga attgaagatg tcattaccga tgttgcaagt
     2341 cgcgctggac aaccagacta tggatagcgc ctacgaaacc actcgcctga ttgccgaaga
     2401 agtcgacatt atcgaagtgg gtaccattct gtgcgtgggc gaaggcgtgc gtgcggttcg
     2461 tgacctgaaa gcgctctacc cgcacaaaat cgtactggca gacgctaaaa ttgctgatgc
     2521 gggcaaaatc ctttcgcgta tgtgcttcga agccaacgct gactgggtga cggtaatttg
     2581 ctgtgcggat atcaacaccg ccaaaggcgc gctggacgta gcaaaagagt ttaacggcga
     2641 cgtgcagatc gaattgaccg gctactggac ctgggaacag gcgcagcagt ggcgtgacgc
     2701 aggcattcag caggtggttt atcaccgcag ccgtgacgcg caggccgcag gcgtagcgtg
     2761 gggcgaagcg gacattaccg cgatcaaacg tctttccgat atgggcttca aagtcaccgt
     2821 caccggtggc ctggcgctgg aagatctgcc gctgttcaaa ggtattccga ttcatgtgtt
     2881 tatcgctggt cgcagcatcc gtgatgccgc ttctccggtt gaagctgcac gtcagttcaa
     2941 acgttccatc gccgaactgt ggggctaagg agcggatatg ttgtccaaac aaatcccgct
     3001 tggcatctat gaaaaagcgc tccccgccgg ggagtgctgg ctggaacgcc tgcaactggc
     3061 aaaaacgttg ggcttcgatt ttgtcgagat gtcggtagat gaaacggacg agcgcctgtc
     3121 gcgcctcgac tggagccgcg agcagcgtct ggcgctggtg aatgcgattg ttgaaaccgg
     3181 cgtgcgcgtg ccgtccatgt gcctttctgc tcatcgtcgt ttcccgctgg gcagtgaaga
     3241 tgacgcggtg cgggcgcagg ggctggagat tatgcgtaaa gctatccagt tcgcccagga
     3301 cgtcggcatt cgcgtgatcc agctggcggg ctatgacgtt tactatcagg aagccaataa
     3361 cgaaacgcgt cgtcgtttcc gtgacggcct gaaagagagc gttgagatgg cgagccgcgc
     3421 gcaggtgacg ctggcgatgg agatcatgga ttatccgttg atgaactcca tcagcaaggc
     3481 gctgggttac gcgcactatc tcaacaaccc gtggttccag ctctacccgg atatcggcaa
     3541 cctgtcggcg tgggacaacg atgtgcagat ggagttgcag gccggaatcg ggcatatcgt
     3601 cgcggtacat gtgaaagaca ccaaacctgg cgtcttcaaa aacgtgccgt ttggcgaagg
     3661 tgtagtggat ttcgaacgtt gtttcgaaac gctcaaacag agtggctatt gcgggccgta
     3721 cctgattgag atgtggagcg aaacggcgga agacccggcg gcagaagtgg cgaaagcgcg
     3781 tgattgggtg aaagcgcgca tggcgaaagc gggcatggtg gaggcggcat aatgcaaaag
     3841 ctaaaacagc aggtatttga agccaacatg gatctgccgc gctacgggct ggtgaccttt
     3901 acctggggca acgtcagcgc tatcgaccgc gaacgcgggc tggtggtgat caagcccagc
     3961 ggcgtcgcct acgaaaccat gaaagcagac gatatggtgg tggtcgatat gagcggtaag
     4021 gtggtggaag gggagtatcg cccatcttcc gacaccgcga cgcatcttga actctaccgt
     4081 cgttatccgt cgcttggcgg cattgtccat acccactcca ctcatgctac cgcatgggcg
     4141 caggcggggc tggcgatccc ggcgttaggc accacgcacg ctgactactt ctttggtgac
     4201 atcccgtgta cgcgtggatt aagcgaagaa gaggtgcagg gtgagtatga actgaacact
     4261 ggcaaagtga ttatcgaaac gctgggcaac gccgagccgc tgcatacgcc gggaattgtg
     4321 gtgtatcagc acggaccgtt cgcctggggg aaagatgccc acgacgcggt gcataacgcg
     4381 gtagtgatgg aagaagtggc gaaaatggcg tggattgcgc gcagtattaa tccacaactc
     4441 aatcacatcg acagcttcct gatgaataaa cacttcatgc gtaagcatgg tcctaacgct
     4501 tattacgggc agaagtaaaa cacgcgctgc ggaaatttcc ttcttcggga gataactggt
     4561 ctaattccgc agccgttttt caaaaaaagc cccctgcgaa gggggcaaag caaactatgg
     4621 caatgtttcg ttggttatac ctggtgctag cgataaatat ccgcgctggc gtgcatattg
     4681 ccgttactcc ccggttcccg catcagaatt acgtggtagt acgttgcgcc ctgcgcatct
     4741 gtttcttcat ttaatgcctg acgtgcttca ctttcagtgg cgaaattatg attgatataa
     4801 atcacgccta agctttgcac atcgtccata tttctggcct ggtggttatt gatttcaatg
     4861 gctgcccatg tatttgcact tagcaaaagc acagccagaa gggctaaaac acgactgaac
     4921 atagatacct cctcaacggc tgactttgtg tgctctcctc tgtgatgatc ttctgattta
     4981 attttaatca atgataaaga agttgatggt gaccatttct gatgcagttg ttcgaaaaaa
     5041 cactgtgacg gaatgtgatg aacttcaaat cagggagtta gttcttttga gcgaaagatg
     5101 agatttattt gtcggctggc cttgagttta gcggggcata caagtactat aacgcgtcat
     5161 ttttcagccg acctttaaca cgttccttgc ctccccggga ttcggctgac ccagacagga
     5221 ggctgaataa tccgtaagga gcaattcgat gcgtcattac gaaatcgttt ttatggtcca
     5281 tcctgaccag agcgaacagg ttccgggcat gatcgagcgc tacactgctg ccatcactgg
     5341 tgcagaaggc aagatccacc gtctggaaga ctggggccgc cgtcagctgg cttacccgat
     5401 caacaaactg cacaaagcac actacgttct gatgaacgtt gaagctccgc aggaagtgat
     5461 cgatgagctg gaaactacct tccgcttcaa cgatgccgtt atccgcagca tggttatgcg
     5521 taccaagcac gctgttaccg aagcatctcc gatggttaaa gcgaaagacg agcgccgtga
     5581 gcgtcgcgat gatttcgcaa acgaaaccgc tgatgatgct gaagctgggg attctgaaga
     5641 gtaatttctg atgaccaacc gtctggtgtt gtccggcacc gtgtgcagga ctccccttcg
     5701 aaaggtcagt ccatcaggaa ttcctcactg ccagttcgtg cttgagcatc gttctgtgca
     5761 ggaggaagcc ggctttcacc ggcaggcatg gtgtcaaatg cccgttattg ttagcggaca
     5821 cgaaaaccag gccattactc acagtataac ggtcggcagt cgcataaccg ttcaggggtt
     5881 catttcatgc cacaaggcaa agaacggact gagcaaaatg gttttgcatg ccgagcagat
     5941 tgaattgata gattctggag actagccata tggcacgtta tttccgtcgt cgcaagttct
     6001 gccgtttcac cgcggaaggc gttcaagaga tcgactataa agatatcgct acgctgaaaa
     6061 actacatcac cgaaagcggt aagattgtcc caagccgtat caccggtacc cgtgcaaaat
     6121 accagcgtca gctggctcgc gctatcaaac gcgctcgcta cctgtccctg ctgccgtaca
     6181 ctgatcgcca tcagtaatcg gtcacggtcc attaatacga ctttgagagg ataaggtaat
     6241 gcaagttatt ctgcttgata aagtagcaaa cctgggtagc ctgggtgatc aggtaaacgt
     6301 taaagcgggc tatgctcgta acttcctggt accgcagggt aaagctgttc cagctaccaa
     6361 gaaaaacatt gaattcttcg aagcacgtcg cgctgaactg gaagctaaac tggctgaagt
     6421 tctggcagct gctaatgctc gcgctgagaa aatcaatgca ctggaaactg ttaccatcgc
     6481 gtctaaagct ggcgacgaag gtaaactgtt cggttccatc ggtactcgcg acatcgctga
     6541 cgctgtaact gcagctggcg ttgaagtggc taagagcgaa gttcgtctgc cgaacggcgt
     6601 tctgcgtacc actggcgaac acgaagtgag cttccaggtt cacagcgaag tattcgcgaa
     6661 agtgatcgta aacgtagtag ctgaataatt cgttattcaa cgagacgtaa aaacgccgac
     6721 cattggtcgg cgttttgctt tctatttttc gtcaggtatt agtttcgcaa gtagatccat
     6781 tcaccattct cttgtgtcca taagtcagga tggtttgctg gcgttagatg catacgatca
     6841 ctccttatgt ctcggtcctg cggcactcgc tcgaaaccta aacccgagta gattgctgct
     6901 gaatttgata aggaacgtag accaattttc aaatcatggg gtaaatcaga atacgcgttt
     6961 agcaatgcag caatgatgaa tccgagggca ttagaaacgc ctgtaacagt gcgaagttgg
     7021 tcgatataaa gatcgattgt tggtagttca gatgaccagc aattaggcat ttgggcagga
     7081 agggttacaa cgtatcctat cgtttggccg gtatagtctg gagtaccata accaggtccc
     7141 aaaaccactt cgtgtattgt gtatttactg ttgggttcga aggattcgac atcttgcaat
     7201 aaatatttca gttctgcggt taacctcgca cccgcgtgag ctttattgta tttctgggcg
     7261 tgaatattat ctaatgtagc tttatggttc catccaaaag aattatcacc attcattgtt
     7321 actccatcgt aagcattaca cttcgttgct aacttattga atgcttgcca gtcagagaga
     7381 tctagtttgt ggatctcaac agtacgtctg ccatcgcaat tatgatctct tccacgattt
     7441 ggcagtaatg tagcacgtgc atattgatga aattttaatt ttactggttt gtcggtggac
     7501 gaaaaatggg gtgaatatgc aggaaatgaa taaatagtcg ttggtaatgt cattgtaatg
     7561 tt
//
LOCUS       DAZKHF020000207         7492 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2201S31241
            SAMN43267800-rid25106413.denovo.209, whole genome shotgun sequence.
ACCESSION   DAZKHF020000207 DAZKHF020000000
VERSION     DAZKHF020000207.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267800
            Sequence Read Archive: SRR30307263
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 7492)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7492)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 7492)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350581.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350581.2
            Genome Coverage       :: 151x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:21:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,652
            CDSs (total)                      :: 5,557
            Genes (coding)                    :: 5,320
            CDSs (with protein)               :: 5,320
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 237
            CDSs (without protein)            :: 237
            Pseudo Genes (ambiguous residues) :: 0 of 237
            Pseudo Genes (frameshifted)       :: 102 of 237
            Pseudo Genes (incomplete)         :: 130 of 237
            Pseudo Genes (internal stop)      :: 52 of 237
            Pseudo Genes (multiple problems)  :: 41 of 237
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7492
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267800-rid25106413.denovo.209"
                     /serotype="O 26:H11"
                     /isolate="2201S31241"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-01"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            102..332
                     /gene="chaB"
                     /locus_tag="ACHOSC_RS18400"
                     /old_locus_tag="pgaptmp_003680"
     CDS             102..332
                     /gene="chaB"
                     /locus_tag="ACHOSC_RS18400"
                     /old_locus_tag="pgaptmp_003680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative cation transport regulator ChaB"
                     /protein_id="HGU4549537.1"
                     /translation="MPYKTKSDLPESVKHVLPSHAQDIYKEAFNSAWDQYKDKDDRRD
                     DASREETAHKVAWAAVKHEYAKGDDDKWHKKS"
     gene            490..1185
                     /gene="chaC"
                     /locus_tag="ACHOSC_RS18405"
                     /old_locus_tag="pgaptmp_003681"
     CDS             490..1185
                     /gene="chaC"
                     /locus_tag="ACHOSC_RS18405"
                     /old_locus_tag="pgaptmp_003681"
                     /EC_number="4.3.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415736.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-specific
                     gamma-glutamylcyclotransferase"
                     /protein_id="HGU4549538.1"
                     /translation="MITRDFLMNADCKTAFGAIEESLLWSAEQRAASLAATLACRPDE
                     GPVWIFGYGSLMWNPALEFTESCTGTLVGWHRAFCLRLTAGRGTAHQPGRMLALKEGG
                     RTTGVAYRLPEETLEQELTLLWKREMITGCYLPTWCQLDLDDGCTVNAIVFIMDPRHP
                     EYESDTRAQVIAPLIAAASGPLGTNAQYLFSLEQELIKLGMQDDGLNDLLVSVKKLLA
                     ENYPDGVLRPGFA"
     gene            complement(1229..1582)
                     /gene="ychN"
                     /locus_tag="ACHOSC_RS18410"
                     /old_locus_tag="pgaptmp_003682"
     CDS             complement(1229..1582)
                     /gene="ychN"
                     /locus_tag="ACHOSC_RS18410"
                     /old_locus_tag="pgaptmp_003682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsrE/F sulfur relay family protein YchN"
                     /protein_id="HGU4549539.1"
                     /translation="MQKIVIVANGAPYGSESLFNSLRLAIALREQESNLDLRLFLMSD
                     AVTAGLRGQKPGEGYNIQQMLEILTAQNVPVKLCKTCTDGRGISTLPLIDGVEIGTLV
                     ELAQWTLSADKVLTF"
     gene            1767..3161
                     /gene="ychO"
                     /locus_tag="ACHOSC_RS18415"
                     /old_locus_tag="pgaptmp_003683"
                     /gene_synonym="iatA"
     CDS             1767..3161
                     /gene="ychO"
                     /locus_tag="ACHOSC_RS18415"
                     /old_locus_tag="pgaptmp_003683"
                     /gene_synonym="iatA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inverse autotransporter invasin YchO"
                     /protein_id="HGU4549540.1"
                     /translation="MSRFVPRIIPFYLLLLVAGGTANAQSTFEQKAANPFDNNNDGLP
                     DLGMAPENHDGEKHFAEIVKDFGETSMNDNGLDTGEQAKAFALGKVRDALSQQVNQHV
                     ESWLSPWGNASVDVKVDNEGHFTGSRGSWFVPLQDNDRYLTWSQLGLTQQDDGLVSNV
                     GVGQRWARGNWLVGYNTFYDNLLDENLQRAGFGAEAWGEYLRLSANFYQPFAAWHEQT
                     ATQEQRMARGYDLTARMRMPFYQHLNTSVSVEQYFGDRVDLFNSGTGYHNPVALSLGL
                     NYTPVPLVTVTAQHKQGESGENQNNLGLNLNYRFGVPLKKQLSAGEVAESQSLRGSRY
                     DNPQRNNLPTLEYRQRKTLTVFLATPPWDLKPGETVPLKLQIRSRYGIRQLIWQGDTQ
                     ILSLTPGAQANSAEGWTLIMPDWQNGEGASNHWRLSVVVEDNQGQRVSSNEITLTLVE
                     PFDALSNDELRWEP"
     gene            complement(3162..3812)
                     /gene="narL"
                     /locus_tag="ACHOSC_RS18420"
                     /old_locus_tag="pgaptmp_003684"
     CDS             complement(3162..3812)
                     /gene="narL"
                     /locus_tag="ACHOSC_RS18420"
                     /old_locus_tag="pgaptmp_003684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator NarL"
                     /protein_id="HGU4549541.1"
                     /translation="MSNQEPATILLIDDHPMLRTGVKQLISMAPDITVVGEASNGEQG
                     IELAESLDPDLILLDLNMPGMNGLETLDKLREKSLSGRIVVFSVSNHEEDVVTALKRG
                     ADGYLLKDMEPEDLLKALHQAAAGEMVLSEALTPVLAASLRANRATTERDVNQLTPRE
                     RDILKLIAQGLPNKMIARRLDITESTVKVHVKHMLKKMKLKSRVEAAVWVHQERIF"
     gene            complement(3805..5601)
                     /gene="narX"
                     /locus_tag="ACHOSC_RS18425"
                     /old_locus_tag="pgaptmp_003685"
     CDS             complement(3805..5601)
                     /gene="narX"
                     /locus_tag="ACHOSC_RS18425"
                     /old_locus_tag="pgaptmp_003685"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate/nitrite two-component system sensor
                     histidine kinase NarX"
                     /protein_id="HGU4549542.1"
                     /translation="MLKRCLSPLTLVNQVALIVLLSTAIGLAGMAVSGWLVQGVQGSA
                     HAINKAGSLRMQSYRLLAAVPLSEKDKPLIKEMEQTAFSAELTRAAERDGQLAQLQGL
                     QDYWRNELIPALMRAQNRETVSADVSQFVAGLDQLVSGFDRTTEMRIETVVLVHRVMA
                     VFMALLLVFTIIWLRARLLQPWRQLLAMASAVSHRDFTQRANISGRNEMAMLGTALNN
                     MSAELAESYAVLEQRVQEKTAGLEHKNQILSFLWQANRRLHSQAPLCERLSPVLNGLQ
                     NLTLLRDIELRVYDTDDEENHQEFTCQPDMTCDDKGCQLCPRGVLPVGDRGTTLKWRL
                     ADSHTQYGILLATLPQGRHLSHDQQQLVDTLVEQLTATLALDRHQERQQQLIVMEERA
                     TIARELHDSIAQSLSCMKMQVSCLQMQGDALPESSRELLSQIRNELNASWAQLRELLT
                     TFRLQLTEPGLRPALEASCEEYSAKFGFPVKLDYQLPPRLVPSHQAIHLLQIAREALS
                     NALKHSQASEVVVTVAQNDNQVKLTVQDNGCGVPENAIRSNHYGMIIMRDRAQSLRGD
                     CRVRRRESGGTEVVVTFIPEKTFTDVQGDTHE"
     gene            5940..7331
                     /gene="narK"
                     /locus_tag="ACHOSC_RS18430"
                     /old_locus_tag="pgaptmp_003686"
     CDS             5940..7331
                     /gene="narK"
                     /locus_tag="ACHOSC_RS18430"
                     /old_locus_tag="pgaptmp_003686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate transporter NarK"
                     /protein_id="HGU4549543.1"
                     /translation="MSHSSAPERATGAVITDWRPEDPAFWQQRGQRIASRNLWISVPC
                     LLLAFCVWMLFSAVAVNLPKVGFNFTTDQLFMLTALPSVSGALLRVPYSFMVPIFGGR
                     RWTAFSTGILIIPCVWLGFAVQDTSTPYSVFIIISLLCGFAGANFASSMANISFFFPK
                     QKQGGALGLNGGLGNMGVSVMQLVAPLVVSLSIFAVFGSQGVKQPDGTELYLANASWI
                     WVPFLAIFTIAAWFGMNDLATSKASIKEQLPVLKRGHLWIMSLLYLATFGSFIGFSAG
                     FAMLSKTQFPDVQILQYAFFGPFIGALARSAGGALSDRLGGTRVTLVNFILMAIFSGL
                     LFLTLPTDGQGGSFMAFFAVFLALFLTAGLGSGSTFQMISVIFRKLTMDRVKAEGGSD
                     ERAMREAATDTAAALGFISAIGAIGGFFIPKAFGSSLALTGSPVGAMKVFLIFYIACV
                     VITWAVYGRHSKK"
BASE COUNT         1740 a         1845 c         1972 g         1935 t
ORIGIN      
        1 agcgtttctt aagaacagtc tgacatgaaa attcttatgt tttggcaaga gtagatattg
       61 ttgaccacac ttaatgttca actttgtaaa aggagtcaac gatgccgtat aaaacgaaaa
      121 gcgatctgcc ggaaagcgta aagcacgttc taccgtctca tgcccaggat atctataaag
      181 aagcgttcaa cagcgcatgg gatcaatata aagataaaga cgatcggcgt gatgacgcca
      241 gtcgcgaaga aaccgcgcat aaagtggcct gggctgctgt gaagcatgaa tatgccaaag
      301 gggatgatga taaatggcat aaaaaatcgt aaaaccggta gcttagttaa agctattcgt
      361 gcggtgttgc cttgcaagtg gtccgtggat tgcatattgt cccgttagtg gtttcaaaat
      421 gagcagtaaa aatgtccgga agacaccaaa aagttgtcgc agggaagtat gcagtggcgg
      481 aggtgtaagg tgataacgcg tgatttcttg atgaatgccg attgtaaaac ggcatttggt
      541 gccattgaag aatcactctt atggtcagca gagcaacggg cggcttcgct ggcggcgacg
      601 ctggcttgtc gacctgatga gggaccggtg tggatcttcg gttatggatc gttgatgtgg
      661 aatccggcac tggagtttac cgagtcgtgc accggtacac tggttggatg gcatcgcgcg
      721 ttttgcctgc gcctgaccgc cgggcgcggg actgcgcacc agccgggacg gatgcttgca
      781 ctgaaagagg gcggacgcac tacaggcgtc gcctatcgac tgccagaaga gacgctggag
      841 caggaactaa ccctgttgtg gaagcgcgag atgattaccg gctgttatct gccaacctgg
      901 tgtcagcttg atcttgatga tggatgcaca gtaaacgcca ttgtgtttat tatggacccg
      961 cgacatccag aatatgaatc tgacactcgc gctcaggtca tcgcgccgtt gattgcggcg
     1021 gcgagcggtc cgctgggaac caacgcacaa tacctgtttt cactggaaca ggagcttatc
     1081 aaactgggaa tgcaggacga tgggctgaat gatttgctgg tatcggtaaa aaaactgctg
     1141 gcggagaatt atccggatgg tgtgttacgg ccgggattcg cctgagtaaa cttccctcat
     1201 agtggggcgt cagacgcccc ctcaaacatt aaaatgtgag cactttatcg gctgacagcg
     1261 tccattgcgc cagttccaca agagtaccga tttccacccc atcaatcaga ggaagtgtac
     1321 taatcccgcg cccgtcggta caggttttgc acaatttcac cggtacattc tgagcggtaa
     1381 ggatctccag catttgctga atgttgtagc cttcccctgg tttttgcccg cgcaacccgg
     1441 ctgtgaccgc atcagacatc aggaacagac gcagatccag attgctctcc tgctctcgta
     1501 acgcaatggc tagccgcaag ctgttaaaca aggattcgct cccgtaaggt gcgccattgg
     1561 caacgatcac gattttttgc attatttact cctgtattca gggaattaga cattcatctt
     1621 ctatcttact gcttctgcag cgtctgacca atcggtcaca tttttaagga ttttcctgaa
     1681 agcgcgagaa aatacgacaa aagttgccag taatcgttat tctttaaggc tatggttttt
     1741 cattattacc ggaagttacc gacgttttga gccgtttcgt tcctcgcatt atcccgtttt
     1801 atttactctt gcttgtagca ggcggtacag ctaacgcaca atctaccttc gagcaaaaag
     1861 cggcaaatcc ctttgataat aacaatgatg gtctgccgga tttaggcatg gctcccgaaa
     1921 atcatgatgg ggaaaaacac tttgctgaaa ttgtgaaaga tttcggcgaa accagtatga
     1981 atgataacgg gctggatact ggcgagcagg caaaagcttt cgcattggga aaagtccgcg
     2041 acgcgcttag tcaacaggtt aatcagcacg tagagtcctg gctatcaccg tggggaaatg
     2101 ccagtgttga cgtcaaagtg gataacgaag gacatttcac cggcagtcgt ggaagctggt
     2161 ttgtgccgtt acaagataat gatcgttatc tcacctggag ccagcttggt cttactcagc
     2221 aggatgatgg gttggtgagc aatgtgggcg ttgggcaacg ctgggcgcgc ggcaactggc
     2281 tggtgggtta taacactttt tatgacaact tgctggacga aaatcttcag cgagcgggct
     2341 ttggtgccga agcgtggggc gaatatttgc gactatcggc aaacttttat cagccgtttg
     2401 ctgcatggca tgaacagaca gccacgcagg aacaacggat ggcgcgcggg tatgacctga
     2461 cagcccggat gcgcatgccg ttctatcaac acctcaatac cagtgtcagc gtagaacagt
     2521 attttggtga tcgtgttgat ttgtttaact ctggtacggg ttatcacaat cccgtcgcgt
     2581 tgagtctggg attaaattac acccctgtgc cattagtcac tgtgacggcc cagcataaac
     2641 agggtgaaag tggcgagaat caaaataacc tcgggctgaa tcttaattac cgctttggtg
     2701 taccgctcaa aaaacaactt tctgcgggtg aggttgccga aagtcagtcg ttacgtggta
     2761 gtcgctatga caatccgcag cgaaataatc taccgactct tgagtaccga cagcgaaaaa
     2821 cgttaacggt gtttctggcg acaccgccgt gggatctaaa acctggcgaa acagtgccgc
     2881 tgaaattaca aatccgcagt cgttacggta ttcggcaact gatttggcag ggcgatacgc
     2941 agatattaag tttgacgccg ggcgcacaag ccaacagcgc ggagggctgg acgctgatca
     3001 tgcctgactg gcagaacggg gaaggggcaa gcaatcactg gcgattgtcg gtggtggtgg
     3061 aagataacca ggggcagcgt gtctcctcca atgagatcac gctaacgctt gtcgaaccgt
     3121 tcgatgcatt gtcaaacgac gaactgcgct gggaaccgta atcagaaaat gcgctcctga
     3181 tgcacccata ccgctgcttc cacgcgagac ttgagcttca ttttcttcag catgtgcttg
     3241 acgtgcactt ttactgtgct ttcggtgata tccaggcggc gggcaatcat cttgttcggc
     3301 aaaccctggg caatcagctt gagaatatcg cgctcgcgtg gggttaactg gttaacatcg
     3361 cgctcagtag tggcacggtt agcgcgcaag ctggcggcca gaacaggcgt taatgcttcg
     3421 cttaatacca tttcgccagc agctgcctga tgcaatgctt tcagcagatc ttccggttcc
     3481 atatctttta acagataacc atccgcgccg cgtttcagtg cggtgaccac atcttcttca
     3541 tggttagaga cgctgaatac cacaatgcgc cctgagaggg acttttcgcg cagtttatcc
     3601 agcgtttcca gaccgttcat gccgggcata ttgagatcta acaggatcag atcgggatca
     3661 agagactccg ccagttcaat accctgttcg ccattactcg cttcgccaac cacggtgata
     3721 tctggtgcca tactgataag ctgttttacg ccagttcgca gcatcgggtg atcgtcaatc
     3781 agcaggatag tagccggttc ctgattactc atgggtatct ccttggacgt ctgtgaaagt
     3841 tttttcggga ataaaggtga ccaccacttc ggtgccacct gattcacgac ggcggacgcg
     3901 gcaatcgcct cgtaaacttt gcgcacggtc gcgcattatt atcatgccgt agtgattgct
     3961 gcggatggca ttttcaggca cgccgcagcc gttatcctgg acggtcagtt tgacctgatt
     4021 atcgttttgc gccaccgtca ccacgacttc actcgcttgc gagtgtttga gggcgttact
     4081 taatgcctca cgggcaattt gcaacaagtg gattgcctga tgcgaaggca ccaggcgagg
     4141 cggcaattga taatccagct tcaccgggaa gccaaatttg gcgctgtact cttcgcaact
     4201 cgcctccagc gccggacgta atccaggctc ggtgagctgc aagcggaatg tggtgagcaa
     4261 ttcacgcaac tgcgcccagg atgcattcag ttcgttacgg atctgactta acagttcgcg
     4321 gctgctttct ggcagcgcat cgccctgcat ctgtaaacaa ctcacctgca tcttcatgca
     4381 agagagagat tgggcaatag aatcatgcag ttcgcgcgca atggtggcac gctcttccat
     4441 cacgatcaac tgttgctgac gttcctgatg gcgatccagc gccagcgtgg cggtgagttg
     4501 ttcaaccagg gtatccacca gttgttgttg atcatggcta agatgacgcc cctgcggcag
     4561 ggtcgccagc aaaataccgt actgcgtatg agagtcagcc agccgccact tcagggttgt
     4621 gccgcgatca ccaacgggta atacgccgcg cgggcagagc tggcagcctt tatcatcaca
     4681 agtcatatct ggttggcagg taaactcctg atgattctct tcatcatcag tatcataaac
     4741 ccgcagttcg atatcacgta gcagggttaa attctgtaag ccgttgagta caggtgacag
     4801 gcgttcacac agcggggctt gggaatgcaa acggcggtta gcctgccata aaaaagagag
     4861 gatctgattt ttatgctcca gcccggcggt tttctcctga acccgctgct caagtacggc
     4921 ataactttcg gccagttctg cagacatatt gttcaacgca gttccaagca tcgccatttc
     4981 gttgcgcccg ctgatgtttg cgcgttgggt aaaatcgcga tgactgacgg cactcgccat
     5041 tgccagcagt tgccgccacg gttgtagcag tcgcgcccgc aaccagataa tagtgaacac
     5101 cagtaaaagt gccataaata ccgccattac ccgatggacc agtaccactg tctcgatgcg
     5161 catttccgtg gtgcggtcaa aaccagatac cagttgatca agcccggcaa caaactggct
     5221 gacatccgct gacaccgttt ctcggttttg tgcacgcatc aacgcaggga tcagttcatt
     5281 acgccagtaa tcttgtaaac cctgtaattg cgccagttgt ccgtctcgtt ctgctgctcg
     5341 agtcaactcg gcgctaaatg ccgtttgttc catctcttta attaagggct tgtctttctc
     5401 gcttaatggc actgctgcca acagacggta actttgcatg cgcagcgatc ccgctttgtt
     5461 gatcgcatgg gcgctgccct gaacgccttg caccagccag ccagaaaccg ccatccctgc
     5521 cagtccaata gcagtagaaa gcaacacaat aagcgcaacc tgattaacca gggtgagcgg
     5581 agagagacaa cgtttaagca tgtaaacctc ttccttcagg ctttaaatga gcaataacct
     5641 taatgaatgt gacgatacat tctggaatgg cagtattctc ggctattggc tgaagtatac
     5701 ccatacctgg aaagagttac tccttatttg ccgtgtggtt agtcgcttta catcgttaag
     5761 ggtagggatt ctacagcacc gtgaaaaatc tcataatttt tatgaaatca ctgtactcac
     5821 tatgggtaat gataaatatc aatgatagat aaagttatct tatcgtttga tttacatcaa
     5881 attgccttta gctacagaca ctaaggtggc agacatcgaa acgagtatca gaggtgtcta
     5941 tgagtcactc atccgccccc gaaagggcta ctggagctgt cattacagat tggcgaccgg
     6001 aagatcctgc gttctggcaa caacgcggtc aacgtattgc cagccgcaac ctgtggattt
     6061 ccgttccctg tctgctgctg gcgttttgcg tatggatgtt gttcagcgct gttgcggtga
     6121 acctaccgaa agtcggcttt aattttacga ccgatcagct atttatgttg actgcgctgc
     6181 cttcggtttc tggcgcgtta ttacgtgttc catactcctt tatggttcct atcttcggtg
     6241 gtcgtcgctg gacggcgttc agcaccggta ttctgattat tccttgcgtc tggctgggtt
     6301 ttgccgtgca ggatacctcc acgccttata gcgtcttcat catcatctct ctgctatgcg
     6361 gctttgctgg cgcgaacttc gcctccagta tggcaaacat cagcttcttc tttccgaaac
     6421 agaagcaggg tggcgcgctg ggtctgaatg gtggtctggg caacatgggc gtcagcgtca
     6481 tgcagttggt tgctccgctg gtggtatcac tgtcgatttt cgcagtattt ggtagccagg
     6541 gcgtcaaaca gccggatggg actgagctgt atctggcgaa tgcgtcctgg atatgggtgc
     6601 cgttccttgc catcttcact attgcggcgt ggtttggcat gaacgatctt gctacctcga
     6661 aagcctccat caaggagcag ttgccggtac tcaaacgggg tcatctgtgg attatgagcc
     6721 tgctgtatct ggcaaccttc ggctccttca tcggcttctc cgcgggcttt gcaatgctgt
     6781 caaaaacgca gttcccggat gttcagattc tgcaatacgc tttcttcggg ccgtttattg
     6841 gtgcgctggc gcgttctgca ggtggtgcat tatctgaccg tctgggcgga actcgtgtca
     6901 cgctggtgaa ctttattctg atggcgattt tcagcggcct gctgttcctg accttaccga
     6961 ctgacgggca gggcggaagc ttcatggcgt tcttcgcggt cttcctggcg ctgttcctga
     7021 cagctgggct gggtagtggt tccactttcc agatgatttc agtgatcttc cgtaaactga
     7081 caatggatcg cgtgaaagca gaagggggtt ctgacgaacg tgcgatgcgt gaagcggcaa
     7141 ccgacacggc agcggcgctg ggtttcatct ctgcgattgg cgctattggt ggcttcttta
     7201 tcccgaaagc gtttggtagc tcgctggcat taacgggttc gccagtcggc gcaatgaagg
     7261 tatttttgat tttctatatc gcctgcgtag tgattacctg ggcggtatat ggtcggcatt
     7321 ctaaaaaata aaccgttact cgtcatactt cgggttacat gtgctgcggc tgcgttcatt
     7381 caccccagtc acttacttta gtaagctcct gggattcatt cacttgccgc cttcctgtaa
     7441 accgaattat atagagtaaa atatttgatt atcctttgcg cggcatgatg tc
//
LOCUS       DAZKHF020000208         7489 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2201S31241
            SAMN43267800-rid25106413.denovo.210, whole genome shotgun sequence.
ACCESSION   DAZKHF020000208 DAZKHF020000000
VERSION     DAZKHF020000208.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267800
            Sequence Read Archive: SRR30307263
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 7489)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7489)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 7489)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350581.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350581.2
            Genome Coverage       :: 151x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:21:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,652
            CDSs (total)                      :: 5,557
            Genes (coding)                    :: 5,320
            CDSs (with protein)               :: 5,320
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 237
            CDSs (without protein)            :: 237
            Pseudo Genes (ambiguous residues) :: 0 of 237
            Pseudo Genes (frameshifted)       :: 102 of 237
            Pseudo Genes (incomplete)         :: 130 of 237
            Pseudo Genes (internal stop)      :: 52 of 237
            Pseudo Genes (multiple problems)  :: 41 of 237
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7489
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267800-rid25106413.denovo.210"
                     /serotype="O 26:H11"
                     /isolate="2201S31241"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-01"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            <1..1109
                     /locus_tag="ACHOSC_RS18435"
                     /old_locus_tag="pgaptmp_003687"
     CDS             <1..1109
                     /locus_tag="ACHOSC_RS18435"
                     /old_locus_tag="pgaptmp_003687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF4049 domain-containing protein"
                     /protein_id="HGU4549544.1"
                     /translation="TMIKPQFADRTYFSNCSPVDPTSTRPTYFGDTDGSVGAVLYALF
                     ATGHLGIMAEGENFLSQLLNIEDEVLNVLLRENFNEQLDTNVNIIISILNRRDIVLES
                     LQPYLVINKDAVTPCTFLGDQTGDRFSNICGDKFIIDLLKRIMSINENVHVLAGNHET
                     NCNGNYMQNFTRMKPLDEDTYAGIKDYPVCFYGPKYKIMANHHGITFDDQRKRYIIGP
                     ITVSSDEMTNALDPVELAAIINKKHHAVINGKKFKNSRAISCRSFNRYFSVSTDYRPK
                     LEALLACSQMLGINQVVAHNGNGGRECIGETGTVLGLNARDSKHAGRMFSMHNCQINP
                     GAGPEITTPWKSYQHEKNRNGLMPLIRRRTMLQL"
     gene            1299..1412
                     /gene="yhiY"
                     /locus_tag="ACHOSC_RS18440"
                     /old_locus_tag="pgaptmp_003688"
     CDS             1299..1412
                     /gene="yhiY"
                     /locus_tag="ACHOSC_RS18440"
                     /old_locus_tag="pgaptmp_003688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YhiY"
                     /protein_id="HGU4549545.1"
                     /translation="MMTTLLPVFTKPSPLALNALRAGRICRFLLIPDGRIR"
     gene            1475..2542
                     /gene="yhiI"
                     /locus_tag="ACHOSC_RS18445"
                     /old_locus_tag="pgaptmp_003689"
     CDS             1475..2542
                     /gene="yhiI"
                     /locus_tag="ACHOSC_RS18445"
                     /old_locus_tag="pgaptmp_003689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family secretion protein"
                     /protein_id="HGU4549546.1"
                     /translation="MDKSKRHLAWWVVGALAVAAVVAWWLLRPAGVPEGFAVSNGRIE
                     ATEVDIASKIAGRIDTILVKEGQFVREGEVLAKMDTRVLQEQRLEAIAQIKEAQSAVA
                     AAQALLEQRQSESRAAQSLVNQRQAELDSVAKRHMRSRSLAQRGAISAQQLDDDRAAA
                     ESARAALESAKAQVSASKAAIEAARTNIIQAQTRVEAAQATERRIAADIDDSELKAPR
                     DGRVQYRVAEPGEVLAAGGRVLNMVDLSDVYMTFFLPTEQAGTLKLGGEARLILDAAP
                     DLRIPATISFVASVAQFTPKTVETSDERLKLMFRVKARIPPELLQQHLEYVKTGLPGV
                     AWVRVNEELPWPDDLVVRLPQ"
     gene            2539..5274
                     /gene="rbbA"
                     /locus_tag="ACHOSC_RS18450"
                     /old_locus_tag="pgaptmp_003690"
     CDS             2539..5274
                     /gene="rbbA"
                     /locus_tag="ACHOSC_RS18450"
                     /old_locus_tag="pgaptmp_003690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated ATPase/putative transporter
                     RbbA"
                     /protein_id="HGU4549547.1"
                     /translation="MTHLELVPVPPVAQLAGVSQHYGKTVALNNITLDIPARCMVGLI
                     GPDGVGKSSLLSLISGARVIEQGNVMVLGGDMRDPKHRRDVCPRIAWMPQGLGKNLYH
                     TLSVYENVDFFARLFGHDKAEREVRINELLTSTGLAPFRDRPAGKLSGGMKQKLGLCC
                     ALIHDPELLILDEPTTGVDSLSRAQFWDLIDSIRQRQSNMSVLVATAYMEEAERFDWL
                     VAMNAGEVLATGSAEELRQQTQSATLEEAFINLLPQAQRQAHQAVVIPPYQPENAEIA
                     IEARDLTMRFGSFVAVDHVNFRIPRGEIFGFLGSNGCGKSTTMKMLTGLLPASEGEAW
                     LFGQPVDPKDIDTRRRVGYMSQAFSLYNELTVRQNLELHARLFHIPEAEIPARVAEMS
                     ERFKLNDVEDVLPESLPLGIRQRLSLAVAVIHRPEMLILDEPTSGVDPVARDMFWQLM
                     VDLSRQDKVTIFISTHFMNEAERCDRISLMHAGKVLASGTPQELVEKRGAASLEEAFI
                     AYLQEAAGQSNEAEAPPVVHDITHAPRQGFSLRRLFSYSRREALELRRDPVRSTLALM
                     GTVILMLIMGYGISMDVENLRFAVLDRDQTVSSQAWTLNLSGSRYFIEQPPLTSYDEL
                     DRRMRAGDITVAIEIPPNFGRDIARGTPVELGVWIDGAMPSRAETVKGYVQAMHQSWL
                     QDVASRQSTPASQSGLMNIETRYRYNPDVKSLPAIVPAVIPLLLMMIPSMLSALSVVR
                     EKELGSIINLYVTPTTRSEFLLGKQLPYIALGMLNFFLLCGLSVFVFGVPHKGSFLTL
                     TLAALLYIIIATGMGLLISTFMKSQIAAIFGTAIITLIPATQFSGMIDPVASLEGPGR
                     WIGEVYPTSHFLTIARGTFSKALDLTDLWQLFIPLLIAIPLVMGLSILLLKKQEG"
     gene            5274..6398
                     /gene="yhhJ"
                     /locus_tag="ACHOSC_RS18455"
                     /old_locus_tag="pgaptmp_003691"
     CDS             5274..6398
                     /gene="yhhJ"
                     /locus_tag="ACHOSC_RS18455"
                     /old_locus_tag="pgaptmp_003691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709253.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HGU4549548.1"
                     /translation="MRHLRNIFNLGIKELRSLLGDKAMLTLIVFSFTVSVYSSATVTP
                     GSLNLAPIAIADMDQSQLSNRIVNSFYRPWFLPPEMITADEMDAGLDAGRYTFAINIP
                     PNFQRDVLAGRQPDIQVNVDATRMSQAFTGNGYIQNIINGEVNSFVARYRDNSEPLVS
                     LETRMRFNPNLDPAWFGGVMAIINNITMLAIVLTGSALIREREHGTVEHLLVMPITPF
                     EIMMAKVWSMGLVVLVVSGLSLVLMVKGVLGVPIEGSIPLFMLGVALSLFATTSIGIF
                     MGTIARSMPQLGLLVILVLLPLQMLSGGSTPRESMPQMVQDIMLTMPTTHFVSLAQAI
                     LYRGAGFEIVWPQFLTLMAIGGAFFTIALLRFRKTIGTMA"
     gene            complement(6563..>7489)
                     /locus_tag="ACHOSC_RS18460"
                     /old_locus_tag="pgaptmp_003692"
     CDS             complement(6563..>7489)
                     /locus_tag="ACHOSC_RS18460"
                     /old_locus_tag="pgaptmp_003692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000420980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ISAs1-like element ISEc1 family transposase"
                     /protein_id="HGU4549549.1"
                     /translation="HDIIARVVSCISPAKFHECFINWMRDCHSSDDKDVIAIDGKTLR
                     HSYDKSRRRGAIHVISAFSTMHSLVLGQIKTDEKSNEITAIPELLNMMDIKGKIITTD
                     AMGCQKDIAEKIQKQGGDYLFAVKGNQGRLNKAFEEKFPLKELNNPEHDSYAMSEKSH
                     GREEIRLHIVCDVPDELIDFTFEWKGLKKLCVAVSFRSIIAEQKKEPEMTVRYYISSA
                     DLTAEKFATAIRNHWHVENKLHWRLDVVMNEDDCKIRKGNAAELFSGIRHIAINILTN
                     DKVFKAGLRRKMRKAAMDRNYLASVLAGSGLS"
BASE COUNT         1755 a         1859 c         2004 g         1871 t
ORIGIN      
        1 aaacaatgat aaaaccccag ttcgctgaca gaacctattt ctccaattgt tcacctgttg
       61 atcccacaag taccagacca acctattttg gcgataccga tggctcagtc ggtgcagttt
      121 tgtacgcgct ttttgcaacg ggacatttgg gaatcatggc tgaaggagaa aatttcctta
      181 gccagttgct taatatagaa gatgaagttc tcaacgtatt gctaagggag aactttaatg
      241 agcaacttga cactaatgtt aatataataa tcagtatcct gaacagaaga gacattgttt
      301 tagaaagtct tcagccttat ttggttatta ataaagacgc agtcacgcca tgtacgtttc
      361 tgggcgacca gactggcgat cgatttagta atatctgtgg cgataaattt atcatcgatt
      421 tattaaaacg cataatgagt atcaatgaaa acgttcatgt attagctgga aatcatgaaa
      481 cgaattgcaa tggtaactat atgcagaact tcacgcgcat gaaaccactt gatgaggata
      541 cctacgctgg gataaaagat tatcctgtat gtttctatgg ccccaaatac aaaataatgg
      601 caaatcatca tgggataact tttgatgatc agcgaaaacg ttacattata ggaccgataa
      661 cagtctctag tgatgaaatg accaatgctc tcgatccggt cgaattagcg gcaattatca
      721 ataaaaagca ccatgctgta attaatggca aaaagtttaa aaatagccgg gctatctctt
      781 gtcgatcctt taatcgttat ttttctgttt cgacagatta cagacctaaa cttgaagcac
      841 tattagcatg ctcacaaatg ttaggtataa atcaagttgt tgcacataac ggtaatggtg
      901 gaagagaatg tataggtgaa acggggacag ttttaggtct taacgcccgt gacagtaaac
      961 atgctggaag aatgttcagc atgcataatt gccaaattaa ccctggtgca gggcctgaga
     1021 taaccactcc ctggaagtct taccagcatg aaaaaaacag aaatggactt atgcctttga
     1081 ttagaaggcg cacaatgtta cagctctgaa ccatagacaa catgaatatt aatgttacta
     1141 tctggttgag actgttgata aaacgtaaaa aggaatgctc tgttcctgca actgaacagg
     1201 cgtgaaacgc gatccaacag gatggatacc gttttgtcag ccagttttat agatcacact
     1261 tatgagtatg cgtgtttcat ttacggccag gaatgctgat gatgacaaca ttactacccg
     1321 ttttcactaa gccttccccg ttggcgctca atgctctgcg cgctggtcgt atttgtcgtt
     1381 tccttcttat cccggatgga agaatccgct gaacgtcgtt cggcggcagt tgtaacacgc
     1441 gtcgtttgct tacagggatt atggcgggcc gattatggat aagagtaagc gccatctggc
     1501 gtggtgggtt gtcggggcac tggcggtggc tgccgtcgtg gcgtggtggc tgttgcgccc
     1561 ggcgggtgtg ccggaaggct ttgctgtcag caatgggcgc attgaagcga cggaagtgga
     1621 tatcgccagc aaaattgccg ggcgtatcga caccattctg gtgaaagaag gccagtttgt
     1681 tcgcgaaggt gaagtgctgg cgaagatgga tactcgcgtg ttgcaggaac agcgactgga
     1741 agccatcgcg caaatcaaag aggcacaaag cgccgttgcc gccgcgcagg ctttgctgga
     1801 gcaacgacaa agcgaaagtc gcgccgcgca gtcgctggtt aatcaacgcc aggcagaact
     1861 ggactccgta gcaaaacgtc atatgcgttc ccgttcactg gcccaacgag gggctatttc
     1921 tgcgcaacag ctggatgacg accgcgccgc cgctgagagc gcccgagctg cgctggaatc
     1981 ggcaaaagct caggtttctg cttctaaagc cgctatagag gcggcacgta ccaatatcat
     2041 tcaggcgcaa acccgcgtcg aagcggcaca agccactgaa cggcgcattg ccgcagatat
     2101 cgatgacagc gaactgaaag ccccgcgtga cggacgcgtg cagtatcggg ttgccgagcc
     2161 aggggaagtg ctggcggcag gcggtcgggt gctgaatatg gtcgatctca gcgacgtcta
     2221 tatgaccttc ttcctgccaa ccgaacaggc gggcacgctg aaactgggtg gtgaagcccg
     2281 gctgatcctc gatgccgcgc cagatctgcg tattccggcg accatcagtt ttgtcgccag
     2341 tgtcgcccag ttcacgccaa aaaccgtcga aaccagcgat gaacggctga aactgatgtt
     2401 ccgcgtcaaa gcgcgtatcc caccggaatt actccagcag catctggaat atgtcaaaac
     2461 cggtttaccg ggcgtagcgt gggtgcgggt gaatgaagaa cttccgtggc ctgacgacct
     2521 cgtggtgagg ttgccgcaat gacgcatctg gaactggttc ccgtcccgcc tgtcgcgcaa
     2581 ctggcgggcg tgagccagca ttatggaaaa accgttgcgc tgaacaatat tactctcgat
     2641 attccggccc gctgtatggt cgggctgatt ggtccggatg gcgtcgggaa gtcgagctta
     2701 ttgtcgttga tttccggtgc ccgcgtcatt gagcagggca atgtgatggt gctgggcggc
     2761 gatatgcgcg acccgaagca tcgccgcgac gtctgcccgc gcatcgcctg gatgccgcag
     2821 gggctgggca aaaatctcta ccacaccttg tcggtgtatg aaaacgtcga ttttttcgct
     2881 cgcctgttcg gtcacgacaa agcggagcgg gaagtgcgaa ttaatgagct gctgaccagc
     2941 accgggttag caccgtttcg cgatcgtccg gcagggaaac tctccggcgg gatgaagcaa
     3001 aaactggggc tgtgctgtgc gttaatccac gacccggaac tgttgattct tgatgagcca
     3061 acaacggggg ttgactcgct ctcccgcgcc cagttctggg atctgatcga cagtattcgc
     3121 cagcggcaga gcaatatgag cgtgctggtt gccaccgcct atatggaaga ggccgaacgc
     3181 ttcgactggt tggtggcgat gaatgccgga gaagtgctgg caaccggcag cgccgaagag
     3241 ttgcggcagc aaacgcaaag cgccacgctg gaagaagcat ttataaatct gttaccgcaa
     3301 gcacaacgcc aggcgcatca ggcggtagtg atcccaccgt atcaacctga aaacgcagag
     3361 attgccatcg aagcgcgcga tctgaccatg cgttttggtt ccttcgttgc cgttgatcac
     3421 gtcaatttcc gcattccacg cggggagatt tttggattcc ttggttcgaa cggctgcggt
     3481 aaatccacca ccatgaaaat gcttaccgga ctgctgcccg ccagcgaagg tgaggcgtgg
     3541 ctgttcgggc aaccggttga tccaaaagat atcgataccc gccgtcgggt aggctatatg
     3601 tcgcaggcgt tttcgctcta taacgaactc accgtgcggc aaaaccttga gttacatgcc
     3661 cgtttgtttc acatcccgga agcggaaatt cccgcgcgag tggctgaaat gagcgagcgt
     3721 tttaagctca acgacgttga agatgttctg ccggagtcat tgccgctcgg cattcgccag
     3781 cggctttcgc tggcggtggc ggtgattcat cgcccggaga tgttaatcct cgatgaacct
     3841 acttctggcg tcgatccggt ggcgagggat atgttctggc agttgatggt cgatctctcg
     3901 cgccaggaca aagtgactat cttcatctcc acccacttta tgaacgaagc ggaacgttgc
     3961 gaccgcatct cactgatgca cgccggaaaa gtgcttgcca gcggtacacc gcaggaactg
     4021 gttgagaaac gcggagccgc cagtctggaa gaggcattta tcgcctattt gcaggaagcg
     4081 gcagggcaga gcaacgaagc cgaagcgccg cccgtggtac acgacatcac ccacgcgccg
     4141 cgtcagggat ttagcctgcg ccgtctgttt agctacagcc gccgcgaagc actggaactg
     4201 cgccgcgatc cggtccgttc gacgctggcg ctgatgggaa cggtgatcct gatgctgata
     4261 atgggttacg gcatcagtat ggatgtggaa aacctgcgct ttgcggtgct cgaccgcgac
     4321 cagaccgtca gtagccaggc gtggacgctc aatctctccg gttcccgtta ctttatcgaa
     4381 cagccgccgc tcaccagtta tgacgagctt gatcgtcgga tgcgtgcggg cgatatcacg
     4441 gtggcaattg agatccctcc caatttcggg cgcgatatcg cgcgtggtac gcctgtggaa
     4501 ctcggcgtct ggattgacgg agcgatgccc agtcgtgccg aaacggtaaa aggttacgtg
     4561 caggcaatgc accagagctg gttacaggat gtggcgagcc gacaatccac acccgccagc
     4621 caaagcgggc tgatgaacat tgagacgcgt tatcgctata acccggacgt aaaaagcctg
     4681 ccagcgattg ttccggcggt gatcccgctt ctgctgatga tgatcccgtc aatgctaagc
     4741 gcccttagcg tggtgcggga aaaagagctt gggtcgatta tcaaccttta cgtgaccccc
     4801 accacgcgta gtgaattttt gcttggtaaa cagttgccat acatcgcgct ggggatgctg
     4861 aactttttcc tgctctgcgg cctgtcggtg tttgtgtttg gcgtaccgca taaaggcagt
     4921 ttcctgacgc tcaccctggc ggcgctgctg tatatcatca ttgccaccgg aatggggctg
     4981 ctgatctcca cctttatgaa aagccagatt gccgccattt tcggaacggc gattatcacg
     5041 ttgatcccgg cgacacagtt ttccgggatg atcgatccgg tagcttcgct ggaagggcct
     5101 ggacgttgga tcggcgaggt ttacccgacc agtcattttc tgactatcgc ccgcgggacg
     5161 ttctcgaaag cgctggatct gactgatttg tggcaacttt ttatcccgtt gctgatagcc
     5221 atcccgctgg tgatgggctt aagtatcctg ctgctgaaaa aacaggaggg atgatgcgcc
     5281 atttacgcaa tatttttaat ctgggtatca aagagttgcg cagtctgctc ggtgataaag
     5341 cgatgctgac gctgattgtc ttctcgttta cggtgtcggt gtattcgtca gcgaccgtta
     5401 cgccaggatc gttgaacctc gcgccgatcg ccattgccga tatggatcaa tcgcagttat
     5461 cgaaccggat cgttaacagc ttctatcgtc cgtggttttt gccaccggag atgatcaccg
     5521 ccgatgagat ggatgccgga ctggacgccg gacgctatac cttcgcgata aatattccgc
     5581 ctaattttca gcgtgatgtc ctcgccggac gccagccaga tattcaggtg aacgtcgatg
     5641 ccacgcgcat gagccaggca tttaccggca atgggtatat ccagaatatt atcaacggtg
     5701 aagtgaacag ctttgtcgcg cgctaccgtg ataacagcga accgttggta tcgctggaaa
     5761 cccggatgcg ctttaacccg aaccttgatc ccgcgtggtt tggcggggtg atggcaatca
     5821 tcaacaacat taccatgctg gcgattgtat tgaccggatc ggcgctgatc cgcgagcgtg
     5881 aacacggcac ggtggaacac ttgttggtga tgccgataac gccgtttgag atcatgatgg
     5941 caaaggtctg gtcgatgggg ctggtggtgc tggtggtgtc gggattatca ctggtgctga
     6001 tggtgaaagg cgtgctgggt gtaccgattg aaggctcgat cccgctgttt atgctgggcg
     6061 tggcgctcag tctgtttgcc accacgtcaa tcggcatttt tatggggacg atagcgcgtt
     6121 caatgccgca actggggctg ctggtgattc tggtgctgct gccgctgcaa atgctttccg
     6181 gtggatccac gccgcgcgaa agtatgccgc agatggtgca ggacattatg ctgaccatgc
     6241 cgacgacaca ctttgttagc ctcgcgcagg ccatcctcta ccggggtgcc ggattcgaaa
     6301 tcgtctggcc gcagttcctg acgctgatgg caattggcgg cgcgtttttc accattgcac
     6361 tgctgcgatt caggaagacg attgggacaa tggcgtaaat acaattgccc gatgcgtaag
     6421 cttataggga ctacgcatcg gtgcaattta ttgaatttta gttgttttta ggccggataa
     6481 ggcgtttatg aactgacccc caaaacctgg acagtttcag ttaaccggct ttttgatact
     6541 cagtcctgta ttcagggcaa gattacgaaa gcccgctccc cgcaaggact gacgccaggt
     6601 agtttctgtc catggctgct tttcgcatct tacgtcttaa ccctgccttg aataccttat
     6661 cattcgtcaa aatattaata gcaatgtgcc gtatccctga aaataattct gctgcatttc
     6721 cttttcttat tttgcagtcg tcttcattca ttaccacgtc cagacgccag tgcagcttat
     6781 tctccacgtg ccagtggttt cggattgctg tggcgaactt ctctgcggtt aaatcagcag
     6841 aactgatata atatctgacc gtcatttctg gctctttctt ttgttctgct attatggacc
     6901 gaaaggagac tgccacgcat aatttcttca gccctttcca ttcaaacgtg aaatcaataa
     6961 gttcatcagg gacatcgcaa acaatatgaa gacggatttc ttctctgccg tgactctttt
     7021 cactcattgc gtaactgtca tgctctggat tatttaattc tttcagcgga aatttttcct
     7081 caaaggcttt atttagccgc ccctggtttc cttttaccgc gaataaataa tcacctccct
     7141 gtttttgtat cttttctgca atatctttct ggcagcccat cgcatcagtt gtgatgattt
     7201 ttcctttaat atccatcatg ttaagaagtt caggaatggc tgtaatctca ttggatttct
     7261 catccgtctt gatctgtccg aggaccagac tatgcattgt tgagaacgca ctaatgacat
     7321 gaatcgctcc cctgcgacga ctcttgtcat aagagtgccg gagcgttttt ccatcaattg
     7381 caatgacgtc tttatcatct gaagaatggc agtcacgcat ccagttaata aagcactcgt
     7441 gaaattttgc aggactgata caggatacaa ctctggcaat gatatcgtg
//
LOCUS       DAZKHF020000209         7450 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2201S31241
            SAMN43267800-rid25106413.denovo.211, whole genome shotgun sequence.
ACCESSION   DAZKHF020000209 DAZKHF020000000
VERSION     DAZKHF020000209.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267800
            Sequence Read Archive: SRR30307263
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 7450)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7450)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 7450)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350581.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350581.2
            Genome Coverage       :: 151x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:21:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,652
            CDSs (total)                      :: 5,557
            Genes (coding)                    :: 5,320
            CDSs (with protein)               :: 5,320
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 237
            CDSs (without protein)            :: 237
            Pseudo Genes (ambiguous residues) :: 0 of 237
            Pseudo Genes (frameshifted)       :: 102 of 237
            Pseudo Genes (incomplete)         :: 130 of 237
            Pseudo Genes (internal stop)      :: 52 of 237
            Pseudo Genes (multiple problems)  :: 41 of 237
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7450
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267800-rid25106413.denovo.211"
                     /serotype="O 26:H11"
                     /isolate="2201S31241"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-01"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            complement(92..915)
                     /locus_tag="ACHOSC_RS18465"
                     /old_locus_tag="pgaptmp_003693"
                     /pseudo
     CDS             complement(92..915)
                     /locus_tag="ACHOSC_RS18465"
                     /old_locus_tag="pgaptmp_003693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414735.4"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="YaeF family permuted papain-like enzyme"
     gene            complement(969..2687)
                     /gene="proS"
                     /locus_tag="ACHOSC_RS18470"
                     /old_locus_tag="pgaptmp_003694"
     CDS             complement(969..2687)
                     /gene="proS"
                     /locus_tag="ACHOSC_RS18470"
                     /old_locus_tag="pgaptmp_003694"
                     /EC_number="6.1.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012133963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004827 - proline-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006433 - prolyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="proline--tRNA ligase"
                     /protein_id="HGU4549550.1"
                     /translation="MRTSQYLLSTLKETPADAEVISHQLMLRAGMIRKLASGLYTWLP
                     TGVRVLKKVENIVREEMNNAGAIEVSMPVVQPADLWQESGRWEQYGPELLRFVDRGER
                     PFVLGPTHEEVITDLIRNELSSYKQLPLNFYQIQTKFRDEVRPRFGVMRSREFLMKDA
                     YSFHTSQESLQETYDAMYAAYSKIFSRMGLDFRAVQADTGSIGGSASHEFQVLAQSGE
                     DDVVFSDTSDYAANIELAEAIAPKEPRAAATQEMTLVDTPNAKTIAELVEQFNLPIEK
                     TVKTLLVKAVEGSSFPLVALLVRGDHELNEVKAEKLPQVASPLTFATEEEIRAVVKAG
                     PGSLGPVNMPIPVVIDRTVAAMSDFAAGANIDGKHYFGINWDRDVATPEVADIRNVVA
                     GDPSPDGQGTLLIKRGIEVGHIFQLGTKYSEALKASVQGEDGRNQILTMGCYGIGVTR
                     VVAAAIEQNYDERGIVWPDAIAPFQVAILPMNMHKSFRVQELAEKLYSELRAQGIEVL
                     LDDRKERPGVMFADMELIGIPHTIVLGDRNLDNDDIEYKYRRNGEKQLIKTGDIVEYL
                     VKQIKG"
     gene            complement(2799..3506)
                     /gene="tsaA"
                     /locus_tag="ACHOSC_RS18475"
                     /old_locus_tag="pgaptmp_003695"
     CDS             complement(2799..3506)
                     /gene="tsaA"
                     /locus_tag="ACHOSC_RS18475"
                     /old_locus_tag="pgaptmp_003695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (N6-threonylcarbamoyladenosine(37)-N6)-methyltransferase
                     TrmO"
                     /protein_id="HGU4549551.1"
                     /translation="MSSFQFEQIGVIRSPYKEKFAVPRQPGLVKSANGELHLIAPYNQ
                     ADAVRGLEAFSHLWILFVFHQTMEGGWRPTVRPPRLGGNARMGVFATRSTFRPNPIGM
                     SLVELKEVVCHKDSVILKLGSLDLVDGTPVVDIKPYLPFAESLPDASASYAQSAPAAE
                     MAVSFTAEVEKQLLTLEKRYPQLTLFIREVLAQDPRPAYRKGEETGKTYAVWLHDFNV
                     RWRVTDAGFEVFALEPR"
     gene            complement(3503..3907)
                     /gene="rcsF"
                     /locus_tag="ACHOSC_RS18480"
                     /old_locus_tag="pgaptmp_003696"
     CDS             complement(3503..3907)
                     /gene="rcsF"
                     /locus_tag="ACHOSC_RS18480"
                     /old_locus_tag="pgaptmp_003696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rcs stress response system protein RcsF"
                     /protein_id="HGU4549552.1"
                     /translation="MRALPICLVALMLSGCSMLSRSPVEPVQSTAPQPKAEPAKPKAP
                     RATPVRIYTNAEELVGKPFRDLGEVSGDSCQASNQDSPPSIPTARKRMQINASKMKAN
                     AVLLHSCEVTSGTPGCYRQAVCIGSALNITAK"
     gene            complement(4025..4840)
                     /gene="metQ"
                     /locus_tag="ACHOSC_RS18485"
                     /old_locus_tag="pgaptmp_003697"
     CDS             complement(4025..4840)
                     /gene="metQ"
                     /locus_tag="ACHOSC_RS18485"
                     /old_locus_tag="pgaptmp_003697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine ABC transporter substrate-binding
                     lipoprotein MetQ"
                     /protein_id="HGU4549553.1"
                     /translation="MAFKFKTFAAVGALIGSLALVGCGQDEKDPNHIKVGVIVGAEQQ
                     VAEVAQKVAKDKYGLDVELVTFNDYVLPNEALSKGDIDANAFQHKPYLDQQLKDRGYK
                     LVAVGNTFVYPIAGYSKKIKSLDELQDGSQVAVPNDPTNLGRSLLLLQKVGLIKLKDG
                     VGLLPTVLDVVENPKNLKIVELEAPQLPRSLDDAQIALAVINTTYASQIGLTPAKDGI
                     FVEDKDSPYVNLIVTREDNKDAENVKKFVQAYQSDEVYEAANKVFNGGAVKGW"
     gene            complement(4880..5533)
                     /locus_tag="ACHOSC_RS18490"
                     /old_locus_tag="pgaptmp_003698"
     CDS             complement(4880..5533)
                     /locus_tag="ACHOSC_RS18490"
                     /old_locus_tag="pgaptmp_003698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine ABC transporter permease MetI"
                     /protein_id="HGU4549554.1"
                     /translation="MSEPMMWLLVRGVWETLAMTFVSGFFGFVIGLPVGVLLYVTRPG
                     QIIANAKLYRTVSAIVNIFRSIPFIILLVWMIPFTRVIVGTSIGLQAAIVPLTVGAAP
                     FIARMVENALLEIPTGLIEASRAMGATPMQIVRKVLLPEALPGLVNAATITLITLVGY
                     SAMGGAVGAGGLGQIGYQYGYIGYNATVMNTVLVLLVILVYLIQFAGDRIVRAVTRK"
     gene            complement(5526..6557)
                     /gene="metN"
                     /locus_tag="ACHOSC_RS18495"
                     /old_locus_tag="pgaptmp_003699"
     CDS             complement(5526..6557)
                     /gene="metN"
                     /locus_tag="ACHOSC_RS18495"
                     /old_locus_tag="pgaptmp_003699"
                     /EC_number="7.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014168731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009276 - Gram-negative-bacterium-type
                     cell wall [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0033232 - ABC-type D-methionine
                     transporter activity [Evidence IEA];
                     GO_process: GO:0048473 - D-methionine transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine ABC transporter ATP-binding protein
                     MetN"
                     /protein_id="HGU4549555.1"
                     /translation="MIKLSNITKVFHQGTRTIQALNNVSLHVPAGQIYGVIGASGAGK
                     STLIRCVNLLERPTEGSVLVDGQELTTLSESELTKARRQIGMIFQHFNLLSSRTVFGN
                     VALPLELDNTPKDEIKRRVTELLSLVGLGDKHDSYPSNLSGGQKQRVAIARALASNPK
                     VLLCDEATSALDPATTRSILELLKDINRRLGLTILLITHEMDVVKRICDCVAVISNGE
                     LIEQDTVSEVFSHPKTPLAQKFIQSTLHLDIPEDYQERLQAEPFTDCVPMLRLEFTGQ
                     SVDAPLLSETARRFNVNNNIISAQMDYAGGVKFGIMLTEMHGTQQDTQAAIAWLQEHH
                     VKVEVLGYV"
     gene            6745..7320
                     /gene="gmhB"
                     /locus_tag="ACHOSC_RS18500"
                     /old_locus_tag="pgaptmp_003700"
     CDS             6745..7320
                     /gene="gmhB"
                     /locus_tag="ACHOSC_RS18500"
                     /old_locus_tag="pgaptmp_003700"
                     /EC_number="3.1.3.82"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-glycero-beta-D-manno-heptose 1,7-bisphosphate
                     7-phosphatase"
                     /protein_id="HGU4549556.1"
                     /translation="MAKSVPAIFLDRDGTINVDHGYVHEIDNFEFIDGVIDAMRELKK
                     MGFALVVVTNQSGIARGKFTEAQFETLTEWMDWSLADRDVDLDGIYYCPHHPQGSVEE
                     FRQVCDCRKPHPGMLLSARDYLHIDMAASYMVGDKLEDMQAAVAANVGTKVLVRTGKP
                     ITPEAENAADWVLNSLADLPQAIKKQQKPAQ"
BASE COUNT         1822 a         1930 c         1892 g         1806 t
ORIGIN      
        1 aatctctgca atctattgaa tttgcatgtt tttgtaggcc ggataaggcg tttacgccgc
       61 atccggcatt ttgtgcacga ggctggcttt atcactgagt cagcccaaca aagcgacccg
      121 ccttgatata aatcccaggc ttcaggtgcc caacatactg taactgcgtt tctggcttaa
      181 acgcagagac atcaccaata cgcatatgca tcagatcggc agggctaatc cagcccgatt
      241 gcgccagtgt aagtggatgc ccggctttgg caaaggcatc cgtgacgaat tccgaacaaa
      301 accacgactt tttgtctcct tcgcccacac tgcttaactg cgctttcgcc aggccgctga
      361 cgcactttgg cgaaaatcct cggagaacgg attcagtgag cacatctggc gagtcaccat
      421 aaagggaata aattcgacaa tgccgcgata gttataaccg ctatctttga ttttattggc
      481 aaacgcggtg atatctgtgg cttgttgcgg ggtaagatcc gggactcgta agacgaaaag
      541 cttatcacta tgcttcatcg cttttttaag ggaaacaatc tggacgccag cgcctgtcgc
      601 ttctgcaacg ttattatcac ccagaaagat tgcaacgtga ctcacagagg aagtgctgaa
      661 gacgcggatt ccaaatgagg ttaccccaag gcttgaggag aacagcaaat cgccgggttt
      721 gagatcaggt gccgttattt cttttattga ttgttcggtg aaagagcttt gatgctggaa
      781 tttgacagcc catgtttttg cctcggcatc tactgctgtg gctgatgggt ctggctggct
      841 gatatcaacg gtacaagccg aaagtaaaag aaagctgggg agaaacaggc ggcagtacgc
      901 ctttggttta tccattttat acaatccatg taaaaaaagg gccctgaaat tcaggaccct
      961 ttctggcatc agcctttaat ctgtttcacc agatattcga cgatgtcacc agtcttaatt
     1021 aactgtttct cgccgttgcg acgatattta tattcgatat cgtcgttgtc gaggttacgg
     1081 tcgcccagca caatagtgtg cggaataccg atcagttcca tatcagcaaa catcacgccc
     1141 ggacgctctt tgcggtcatc cagcagcact tcgatacctt gtgcacgcag ttcgctgtac
     1201 agtttctcag caagctcttg tacgcggaag gatttgtgca tgttcatcgg cagaatcgcc
     1261 acctggaacg gcgcgatagc gtcaggccat acgatgcctc gttcgtcgta gttctgctca
     1321 atcgccgcag ctaccacacg cgttaccccg ataccgtagc aacccatcgt caggatttgg
     1381 ttacggccat cttcaccctg tacggaggct ttcagtgctt cggagtactt ggtacccagc
     1441 tggaagatgt gaccaacttc gataccacgt ttgatcagca gcgtaccctg gccatccggg
     1501 cttggatcgc cagccaccac gttacggata tctgcaactt ccggggtagc gacatcgcga
     1561 tcccagttga tgccgaagta gtgtttacca tcgatgttag caccagcagc gaaatcactc
     1621 atcgccgcaa cggtacggtc aatcaccacc ggaatcggca tgtttaccgg acccagtgaa
     1681 cccggaccgg ctttaaccac ggcacgaatt tcttcttcgg tcgcgaaagt cagcgggctt
     1741 gcaacctgcg gcagtttttc tgctttaact tcgttcagct cgtgatcacc gcgcaccagc
     1801 agcgcaacca gcgggaagct actgccttca accgctttaa ccagcagagt cttaaccgtt
     1861 ttctcaatcg gcagattgaa ctgttcaacc agttccgcga tggttttcgc gttcggcgta
     1921 tcaaccagcg tcatttcctg ggtagcagca gcgcgcggtt ctttcggcgc gatagcttct
     1981 gccagttcaa tgttcgctgc atagtcagag gtgtcggaga agaccacatc gtcttcaccg
     2041 ctctgcgcca gcacctggaa ttcgtgagag gcgctgccgc cgatagaacc ggtgtcggct
     2101 tgtacggcgc ggaaatccag ccccatgcgg ctgaagattt tgctgtaggc cgcatacatt
     2161 gcatcgtagg tttcctgcag ggattcctga gaagtatgga aagagtaagc atctttcatc
     2221 aggaattcgc gggaacgcat gacgccgaaa cgcggacgca cttcgtcgcg gaacttggtc
     2281 tggatctgat agaagttcag cggcagctgt ttgtaagagc taagctcgtt acgaatcagg
     2341 tcagtgataa cttcttcatg agttgggccg agtacgaacg gacgctcgcc acggtcaaca
     2401 aaacgcagca gttccggacc gtactgttcc caacgaccac tctcttgcca caaatcagct
     2461 ggctgaacca ccggcatcga cacctcgatc gcaccggcgt tgttcatctc ttcacgcacg
     2521 atgttttcga cttttttcag aacgcgcacg ccggtcggca gccaggtata taacccggag
     2581 gccagcttgc ggatcatccc ggcgcgcagc atcagctgat ggctgatcac ctcggcgtcg
     2641 gcaggtgtct ccttgagagt ggagagcagg tattggctag tacgcatgtt gttacggttc
     2701 cagttggaag gtagaacagg ctcaaggcga gcctgggaca aaaaaaagtg atttagttta
     2761 ccagtgcaaa agaaatgtca aaagagaagg gcgtgaattt aacgcggttc cagcgcaaag
     2821 acttcaaaac ctgcgtcggt gacgcgccag cgaacgttaa aatcatgtag ccagacggca
     2881 taggttttgc ccgtttcctc acctttacga taggccgggc gtgggtcctg cgccagtact
     2941 tcgcggataa acagcgttaa ctgcggataa cgcttctcca gcgtcaaaag ctgcttttcg
     3001 acctctgcgg taaaactcac cgccatctct gccgctggcg cactttgcgc atagctggca
     3061 ctggcatcgg gaagcgattc ggcaaagggg agatacggtt tgatatccac taccggcgta
     3121 ccatcgacca gatccagact gccgagcttc agaatcacgc tgtctttatg gcaaacaacc
     3181 tctttcagct ctaccagcga cataccaatt gggttagggc ggaaagtaga gcgtgttgcg
     3241 aaaaccccca ttctggcgtt accgccgagg cgcggtggac gcacagtcgg acgccagccg
     3301 ccttccatcg tttgatgaaa gacgaaaagg atccataaat ggctgaacgc ttccaggccg
     3361 cgaacggcgt cggcctggtt gtagggagca atgagatgca gttcgccgtt ggcgcttttt
     3421 accagacctg gctggcgcgg aacggcgaat ttttctttat agggcgagcg aataacgcct
     3481 atttgctcga actggaaact gctcatttcg ccgtaatgtt aagcgcagag ccgatacata
     3541 cagcctgacg atagcagcct ggcgtaccgc tggtgacttc gcagctatgc agtaataccg
     3601 cattggcctt cattttcgag gcgttgattt gcatccgctt acgcgcggtt ggaatgctcg
     3661 gcggagagtc ctgattagag gcctggcaag agtcgccact gacttcaccg agatcgcgga
     3721 acggtttgcc gactaattct tctgcattgg tataaattcg gaccggcgtg gcgcgcggcg
     3781 ctttcggttt tgcaggctcc gctttcggct ggggtgcagt gctttgaacg ggttcgacag
     3841 gggatctgct taacatggaa cagccgctta gcatgagtgc tactaaacag atcggtaaag
     3901 cacgcatagt atttcctcaa tgtatgatca aaacgtcaat attgaatcag gagcttgtaa
     3961 aaatgacaag acgggcaagc gcccgtcctg aatgatatta caaattgtgg aaacagccta
     4021 aaaattacca gcctttaaca gcgccgccgt taaacacttt gtttgctgct tcgtaaactt
     4081 catcagactg ataagcctgg acgaatttct tcacgttctc ggcgtctttg ttatcttcac
     4141 gcgttacgat caggtttacg tacggggagt ctttatcttc gacaaagata ccgtctttcg
     4201 ctggagtcag gccaatctgg ctggcatagg tggtattgat aactgccaga gcgatttgcg
     4261 cgtcgtccag agatcgaggc agctgcggtg cttccagctc aacaattttc agattttttg
     4321 ggttctcaac aacatcaaga acggtcggca gcaggccaac gccatctttc agtttgatca
     4381 agcccacttt ttgcagcagc agcagtgaac gaccaagatt agttggatcg tttggcacgg
     4441 caacctgcga accatcctgc agttcatcca gtgatttgat tttcttggag taaccagcaa
     4501 tcggataaac aaaagtgttg cctactgcga ccagtttgta gccacgatct ttcagttgct
     4561 gatcaaggta cggtttatgc tggaaggcgt tggcgtcgat atcgcctttg ctcaatgctt
     4621 cgtttggcag aacatagtcg ttgaaggtta ccagctcaac gtccaggcca tatttgtctt
     4681 tcgcaacttt ctgcgcgact tctgctacct gctgttcggc accaacaatc acgccgactt
     4741 taatgtggtt tggatctttt tcatcctgac cgcagcctac cagtgccagt gatccaatca
     4801 gggctcccac tgccgcaaag gttttgaatt tgaacgccat accttattcc ttcttcaatt
     4861 atttatgttg tgttgaacgt tacttgcgag tgacagcccg gacgatgcgg tcgcctgcga
     4921 actgaattaa ataaaccaga atgaccagca ataccagtac cgtattcatc accgtcgcgt
     4981 tatagccgat gtagccatac tgatagccaa tctgacctaa accaccggca ccgactgcac
     5041 cacccatcgc ggaataaccg accagggtaa tcagggtgat agttgccgca ttcaccagac
     5101 ccggcaatgc ttccggtaac agcactttac ggacgatctg catcggcgtg gcacccattg
     5161 cacgggaagc ttcaattaac ccggttggga tctccagcag agcgttctcg accatacggg
     5221 caataaacgg tgctgcacca acggttaacg gaacaatcgc tgcctgcaaa ccaatcgatg
     5281 taccgacaat aacgcgggta aacggaatca tccataccag caagataatg aacgggatgg
     5341 aacggaaaat gttcacaatc gcagaaacgg tacgatacag cttcgcgtta gcaataattt
     5401 gccccgggcg cgtaacataa agcaggacgc cgaccggcag accaatcaca aagccaaaaa
     5461 atccggatac gaaggtcatt gccagcgttt cccatacgcc acgaaccagc aaccacatca
     5521 tcggctcaga cataacccag tacctctact tttacatggt gttcctgcag ccaggcaatg
     5581 gcggcttgcg tatcttgttg tgtgccgtgc atttcagtca gcatgatgcc gaacttaacg
     5641 ccaccggcgt aatccatctg cgcgctaata atgttgttgt tgacgttgaa acgacgcgcg
     5701 gtttcagaaa gcagcggggc atcgaccgat tgaccggtaa actccaggcg caacatcggc
     5761 acgcagtcag taaatggctc cgcttgcaga cgttcctggt aatcttccgg gatatccaga
     5821 tgcagggtcg actgaataaa cttctgcgcc agcggcgttt tcggatgcga gaacacttca
     5881 cttaccgtgt cctgctcgat cagttcgcca ttgctgatga ccgccacgca atcacaaata
     5941 cgcttcacaa cgtccatttc gtgggtgatc aacagaatcg tcaaacccag acggcggtta
     6001 atgtctttca gcagttcgag aatagaacgt gtcgttgccg ggtccagcgc gctggtggct
     6061 tcatcacaca gcaatacttt gggattgctg gctaatgcac gggcaatcgc cacacgttgt
     6121 ttctgcccac cggaaagatt cgacgggtag ctatcatgct tatcgccaag accaaccaat
     6181 gacagcaatt ccgtcacgcg acgtttgatc tcgtctttcg gtgtgttgtc cagctccagc
     6241 ggcagagcca cgttgccaaa aacagtacgc gaagagagca ggttaaaatg ctggaaaatc
     6301 ataccaatct ggcggcgagc tttggtcaac tcggattctg acagcgtggt cagttcctgg
     6361 ccatcgacca gcacgctacc ctcggttggg cgctccagca ggtttacaca acgtataagc
     6421 gtactcttac ccgcgcctga ggcaccgata acgccataaa tttgtccagc tggcacatgc
     6481 aggctgacgt tgttcaacgc ctggatggtg cgggtgccct ggtggaacac tttggtgata
     6541 ttcgaaagtt ttatcattga ttatttatta tcgtcattaa gttagtcgtg gcatctcgaa
     6601 tgcctgaaac gggcaacgcc gtcaatgaaa tggatgttaa ggcatccaga cgtctaaatc
     6661 aatcaggttt atgcgaagag cactttcttg caggtcgaaa catgcgatac tagcgtccca
     6721 tgccttatta aggagctata aaaggtggcg aagagcgtac ccgcaatttt tcttgaccgt
     6781 gatggcacca ttaatgtcga tcacggctat gtccatgaga tcgacaactt tgaatttatc
     6841 gacggtgtta ttgacgccat gcgcgagcta aaaaaaatgg gctttgcgct ggtggtagta
     6901 accaaccagt ctggcattgc tcgcggtaaa tttaccgaag cacagtttga aacgctgacc
     6961 gagtggatgg actggtcgct ggcggaccga gatgtcgatc tggatggtat ctattattgc
     7021 ccgcatcatc cgcagggtag tgttgaagag tttcgccagg tctgcgattg ccgcaaacca
     7081 catccgggga tgcttttgtc agcacgcgat tatttgcata ttgatatggc cgcttcttat
     7141 atggtgggcg ataaattaga agatatgcag gcagcggttg cggcgaacgt gggaacaaaa
     7201 gtgctggtgc gtacgggtaa acctattaca cctgaagcag aaaacgcggc agattgggtg
     7261 ttaaatagcc tggcagacct gccgcaagcg ataaaaaagc agcaaaaacc ggcacaatga
     7321 ttaaaagatg agcggttgaa ataaaaatgc atttttccgc ttgtcttcct gagccgactc
     7381 cctataatgc gcctccatcg acacggcgga tgtgaatcac ttcacacaaa cagccggttc
     7441 ggttgaagag
//
LOCUS       DAZKHF020000210         7363 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2201S31241
            SAMN43267800-rid25106413.denovo.212, whole genome shotgun sequence.
ACCESSION   DAZKHF020000210 DAZKHF020000000
VERSION     DAZKHF020000210.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267800
            Sequence Read Archive: SRR30307263
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 7363)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7363)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 7363)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350581.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350581.2
            Genome Coverage       :: 151x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:21:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,652
            CDSs (total)                      :: 5,557
            Genes (coding)                    :: 5,320
            CDSs (with protein)               :: 5,320
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 237
            CDSs (without protein)            :: 237
            Pseudo Genes (ambiguous residues) :: 0 of 237
            Pseudo Genes (frameshifted)       :: 102 of 237
            Pseudo Genes (incomplete)         :: 130 of 237
            Pseudo Genes (internal stop)      :: 52 of 237
            Pseudo Genes (multiple problems)  :: 41 of 237
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7363
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267800-rid25106413.denovo.212"
                     /serotype="O 26:H11"
                     /isolate="2201S31241"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-01"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            24..854
                     /locus_tag="ACHOSC_RS18505"
                     /old_locus_tag="pgaptmp_003701"
     CDS             24..854
                     /locus_tag="ACHOSC_RS18505"
                     /old_locus_tag="pgaptmp_003701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000843961.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR family DNA-binding transcription
                     regulator"
                     /protein_id="HGU4549557.1"
                     /translation="MKSVNVPRKSSNLLKRRLKIAEIVAAQGEIKVDDLSAQLGVSGV
                     TIRGDLSYLEQQGYLKRSFGGAIATPLSSEPEAIQPIAPLSLAQQMEIARHCARLIRD
                     RDTLFLGHGTICRKIIPLLSEVKKLRLITNDPEHALLANQFIDGEIILAGSEMLRPDT
                     ALAGKPLEQALQHFTITHSILEISHLDADGTLNINVPRLAAAWQLCFDHAQNKTVIVA
                     ADPAPSTAAIGHLHQAENVIVSRSINEQYQQQLQSADFVILYTSNECFTWSNRERLQE
                     "
     gene            886..1821
                     /locus_tag="ACHOSC_RS18510"
                     /old_locus_tag="pgaptmp_003702"
     CDS             886..1821
                     /locus_tag="ACHOSC_RS18510"
                     /old_locus_tag="pgaptmp_003702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001446002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0019200 - carbohydrate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar kinase"
                     /protein_id="HGU4549558.1"
                     /translation="MGELLAEFLSRNPHQKFTQPGEFIGPFPSGAPAIFAAQVAKLSH
                     RAIFFGCVGNDDFGRLIIERLRHEGVITDGIHVMNNAVTGTAFVSYQNPQQRDFVFNI
                     PNSACGLFTAEHIDKDLLKQCNHLHIVGSSLFSFRMIDVMRKAITTIKSAGGTVSFDP
                     NIRKEMLSIPEMAQALDYLIEYTDIFIPSESELPFFARHKNLSEEQIVSDLLHGGVKH
                     VAIKRAQRGASYYKLKNGTLHAQHVTGHDIEIIDPTGAGDCFGATFITLFLSGFPAHK
                     ALQYANASGALAVMRQGPMEGISSLADIEDFLQQH"
     gene            1914..2156
                     /gene="yhdT"
                     /locus_tag="ACHOSC_RS18515"
                     /old_locus_tag="pgaptmp_003703"
     CDS             1914..2156
                     /gene="yhdT"
                     /locus_tag="ACHOSC_RS18515"
                     /old_locus_tag="pgaptmp_003703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhdT family protein"
                     /protein_id="HGU4549559.1"
                     /translation="MDTRFVQAHKEARWALGLTLLYLAVWLVAAYLPGVAPGFTGFPR
                     WFEMACILTPLLFIGLCWAMVKFIYRDIPLEDDDAA"
     gene            2146..3597
                     /gene="panF"
                     /locus_tag="ACHOSC_RS18520"
                     /old_locus_tag="pgaptmp_003704"
     CDS             2146..3597
                     /gene="panF"
                     /locus_tag="ACHOSC_RS18520"
                     /old_locus_tag="pgaptmp_003704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312157.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015081 - sodium ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0015233 - pantothenate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015887 - pantothenate transmembrane
                     transport [Evidence IEA];
                     GO_process: GO:0036376 - sodium ion export across plasma
                     membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sodium/pantothenate symporter"
                     /protein_id="HGU4549560.1"
                     /translation="MQLEVILPLVAYLVVVFGISVYAMRKRSTGTFLNEYFLGSRSMG
                     GIVLAMTLTATYISASSFIGGPGAAYKYGLGWVLLAMIQLPAVWLSLGILGKKFAILA
                     RRYNAVTLNDMLFARYQSRLLVWLASLSLLVAFVGAMTVQFIGGARLLETAAGIPYET
                     GLLIFGISIALYTAFGGFRASVLNDTMQGLVMLIGTVVLLIGVVHAAGGLSNAVQTLQ
                     TIDPQLVTPQGADDILSPAFMTSFWVLVCFGVIGLPHTAVRCISYKDSKAVHRGIIIG
                     TIVVAILMFGMHLAGALGRAVIPDLTVPDLVIPTLMVKVLPPFAAGIFLAAPMAAIMS
                     TINAQLLQSSATIIKDLYLNIRPDQMQNETRLKRMSAVITLVLGALLLLAAWKPPEMI
                     IWLNLLAFGGLEAVFLWPLVLGLYWERANAKGALSAMIVGGVLYAVLATLNIQYLGFH
                     PIVPSLLLSLLAFLVGNRFGTSVPQATVLTTDK"
     gene            3609..4490
                     /gene="prmA"
                     /locus_tag="ACHOSC_RS18525"
                     /old_locus_tag="pgaptmp_003705"
     CDS             3609..4490
                     /gene="prmA"
                     /locus_tag="ACHOSC_RS18525"
                     /old_locus_tag="pgaptmp_003705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11 methyltransferase"
                     /protein_id="HGU4549561.1"
                     /translation="MPWIQLKLNTTGANAEDLSDALMEAGAVSITFQDTHDTPVFEPL
                     PGETRLWGDTDVIGLFDAETDMNDVVAILENHPLLGAGFAHKIEQLEDKDWEREWMDN
                     FHPMRFGERLWICPSWRDVPDENAVNVMLDPGLAFGTGTHPTTSLCLQWLDSLDLTGK
                     TVIDFGCGSGILAIAALKLGAAKAIGIDIDPQAIQASRDNAERNGVSDRLELYLPKDQ
                     PEEMKADVVVANILAGPLRELAPLISVLPVSGGLLGLSGILASQAESVCEAYADSFAL
                     DPVVEKEEWCRITGRKN"
     gene            4819..5784
                     /gene="dusB"
                     /locus_tag="ACHOSC_RS18530"
                     /old_locus_tag="pgaptmp_003706"
     CDS             4819..5784
                     /gene="dusB"
                     /locus_tag="ACHOSC_RS18530"
                     /old_locus_tag="pgaptmp_003706"
                     /EC_number="1.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008785970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0017150 - tRNA dihydrouridine synthase
                     activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA dihydrouridine synthase DusB"
                     /protein_id="HGU4549562.1"
                     /translation="MRIGQYQLRNRLIAAPMAGITDRPFRTLCYEMGAGLTVSEMMSS
                     NPQVWESDKSRLRMVHIDEPGIRTVQIAGSDPKEMADAARINVESGAQIIDINMGCPA
                     KKVNRKLAGSALLQYPDVVKSILTEVVNAVDVPVTLKIRTGWAPEHRNCEEIAQLAED
                     CGIQALTIHGRTRACLFNGEAEYDSIRAVKQKVSIPVIANGDITDPLKARAVLDYTGA
                     DALMIGRAAQGRPWIFREIQHYLDTGELLPPLPLAEVKRLLCAHVRELHDFYGPAKGY
                     RIARKHVSWYLQEHAPNDQFRRTFNAIEDASEQLEALEAYFENFA"
     gene            5810..6106
                     /gene="fis"
                     /locus_tag="ACHOSC_RS18535"
                     /old_locus_tag="pgaptmp_003707"
     CDS             5810..6106
                     /gene="fis"
                     /locus_tag="ACHOSC_RS18535"
                     /old_locus_tag="pgaptmp_003707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001465625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator Fis"
                     /protein_id="HGU4549563.1"
                     /translation="MFEQRVNSDVLTVSTVNSQDQVTQKPLRDSVKQALKNYFAQLNG
                     QDVNDLYELVLAEVEQPLLDMVMQYTRGNQTRAALMMGINRGTLRKKLKKYGMN"
     gene            6192..7076
                     /gene="yhdJ"
                     /locus_tag="ACHOSC_RS18540"
                     /old_locus_tag="pgaptmp_003708"
     CDS             6192..7076
                     /gene="yhdJ"
                     /locus_tag="ACHOSC_RS18540"
                     /old_locus_tag="pgaptmp_003708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417728.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008170 - N-methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenine-specific DNA-methyltransferase"
                     /protein_id="HGU4549564.1"
                     /translation="MRTGCEPTRFGNEAKTIIHGDALAELKKIPAESVDLIFADPPYN
                     IGKNFDGLIEAWKEDLFIDWLFEVIAECHRVLKKQGSMYIMNSTENMPFIDLQCRKLF
                     TIKSRIVWSYDSSGVQAKKHYGSMYEPILMMVKDAKNYTFNGDAILVEAKTGSQRALI
                     DYRKNPPQPYNHQKVPGNVWDFPRVRYLMDEYENHPTQKPEALLKRIILASSNPGDIV
                     LDPFAGSFTTGAVAVSSGRKFIGIEINSEYIKMGLRRLDVASHYSAEELAKVKKRKTG
                     NLSKRSRLSEVDPDLIAK"
     gene            7160..7339
                     /gene="yhdU"
                     /locus_tag="ACHOSC_RS18545"
                     /old_locus_tag="pgaptmp_003709"
     CDS             7160..7339
                     /gene="yhdU"
                     /locus_tag="ACHOSC_RS18545"
                     /old_locus_tag="pgaptmp_003709"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2556 family protein"
                     /protein_id="HGU4549565.1"
                     /translation="MIRKYWWLVVFAVFVFLFDTLLMQWIELLATETDKCRNMNSVNP
                     LKLVNCDELNFQDRM"
BASE COUNT         1798 a         1802 c         1890 g         1873 t
ORIGIN      
        1 atagtgttac gcattaaagg ccaatgaaga gtgttaacgt gccgagaaaa tcgtctaacc
       61 tactgaaacg cagattaaaa attgcagaaa ttgttgccgc ccaaggtgaa atcaaagttg
      121 atgacttatc tgcgcaactt ggtgtctctg gcgtgacgat ccgcggcgat ctcagttatc
      181 tggagcaaca gggatattta aagcgatcat ttggcggtgc tattgcgaca ccactctcct
      241 ctgaaccaga agcgatacaa ccgatcgcgc cattgtctct ggcacaacaa atggaaatag
      301 cacgacattg cgcacggtta attcgcgatc gtgacacgct atttcttgga catgggacaa
      361 tctgccgtaa gatcattcca ttgctcagcg aagtgaaaaa actgcgtctg atcaccaacg
      421 atcccgaaca tgcgctgctg gcaaatcagt ttattgatgg cgaaataata ctggctggca
      481 gtgaaatgct gcgccccgac accgcactgg ccggaaaacc gcttgaacag gcacttcagc
      541 atttcaccat tacacatagc attctggaaa tcagccatct ggatgctgat ggtactttaa
      601 atatcaacgt tcccagactg gccgctgcct ggcagctctg tttcgatcat gcccagaata
      661 agacggttat cgttgccgcc gatcctgcgc catcaacggc tgctatcggg catttgcatc
      721 aggcagaaaa tgtgatcgtc agccgcagca ttaacgaaca atatcagcaa caattgcagt
      781 cggctgattt cgttatcctt tataccagta atgagtgctt tacctggtcc aatcgcgaaa
      841 ggcttcagga atgagagggg ttttgtgaca ataactatct gcgctatggg cgaattgctg
      901 gccgagtttt tgtcccgcaa cccacatcaa aaattcactc agcctgggga gtttatcggg
      961 ccatttccca gcggtgcgcc agcaattttt gctgctcagg tggcaaaact gtcccatcgg
     1021 gccatcttct tcggatgtgt tggtaatgat gattttggcc gactcattat agagcgcctc
     1081 cgtcatgaag gtgtcattac cgatgggatc catgttatga acaatgccgt cacaggtacg
     1141 gcgttcgtga gttatcaaaa tccccagcag cgcgatttcg tctttaatat ccctaacagc
     1201 gcctgcggct tgtttactgc tgagcacatt gataaggatc tgctaaaaca gtgtaaccat
     1261 ctccatattg tgggctcatc gttgttctca tttcgcatga tcgatgtcat gcgtaaagca
     1321 ataacgacga tcaaatcggc tggcggcacc gtttctttcg atcccaatat tcgcaaagag
     1381 atgctgagca ttcctgaaat ggcgcaggct ctcgattatt tgattgaata tacggatatt
     1441 ttcatcccca gcgaaagcga actccctttc ttcgcgcgtc acaaaaatct gtcagaagaa
     1501 cagattgtta gcgatcttct ccacggcggc gtaaaacatg tggcgataaa acgcgcccag
     1561 cgtggggcca gctattacaa gcttaaaaac ggtacattac acgcccagca tgttacaggc
     1621 cacgatatcg aaattatcga tccaacgggt gcaggcgact gctttggcgc aacgtttatc
     1681 actcttttct tatccggttt cccggcacat aaggcactgc aatatgcaaa tgccagcggc
     1741 gcgctcgccg taatgcggca aggtccgatg gaagggatat cctcactggc tgacattgaa
     1801 gactttttgc agcagcactg aacggcaatt ttctgtcaga ttattttccc tgcctgaaga
     1861 gttgccgtag aattgcccgt ttctcgtatc cccccatgag gaggaaccct tccgtggaca
     1921 ctcgttttgt tcaggcccat aaagaggcgc gctgggcgct ggggctgacc cttttgtatc
     1981 tggcagtttg gttagtagcc gcttacttac ctggcgttgc ccccggtttt accggctttc
     2041 cgcgctggtt tgagatggcc tgcatcctga cgccgctgct gtttattgga ctgtgctggg
     2101 cgatggtgaa atttatctat cgcgatatcc cactggagga tgacgatgca gcttgaagta
     2161 attctaccgc tggtcgccta tctggtggtg gtgttcggta tctcggttta tgcgatgcgt
     2221 aaacggagca ccggcacctt ccttaatgag tatttcctcg gcagccgctc tatgggcggt
     2281 attgtgctgg cgatgacgct caccgcgacc tatatcagtg ccagttcgtt tatcggcggg
     2341 ccaggagctg cttataaata cgggctgggc tgggtattgc tggcgatgat tcagcttcct
     2401 gcagtctggc tttcactcgg tattctcggc aagaagtttg cgattcttgc gcgccgctac
     2461 aatgcagtga cgctgaacga tatgctgttt gcccgctacc agagtcgtct tctggtgtgg
     2521 ctggcgagtt tgagtttgct ggttgcgttc gttggtgcga tgaccgtgca gtttatcggc
     2581 ggtgcgcgcc tgctggaaac cgcggcgggt attccttatg aaaccgggct gctgattttt
     2641 ggtatcagca ttgcgttata taccgccttt ggtggctttc gcgccagcgt gctgaacgac
     2701 accatgcaag ggcttgtgat gctgattggc accgttgtgc tgcttattgg cgtggtacat
     2761 gccgctggcg gcttaagcaa cgcagtacag accttgcaaa ccatcgatcc gcaactggtt
     2821 acgccacaag gcgctgacga tattctgtcg cctgccttta tgacgtcgtt ctgggtactg
     2881 gtgtgctttg gcgtgattgg cctgccgcat actgcggtgc gctgtatctc ttataaagac
     2941 agcaaagcag tacaccgggg gatcatcatc ggtacgattg tagtcgcaat cctgatgttc
     3001 ggtatgcacc tggccggagc gttaggtcgt gcagtgatcc ccgatctcac cgtaccggat
     3061 ctggtgatcc cgacgttaat ggtaaaagtg ctgccaccgt ttgctgccgg aatcttcctg
     3121 gctgcaccta tggctgcgat catgtcgacg atcaatgccc agttgctgca aagttccgct
     3181 acgatcatta aagatctcta tctgaatatc cgtccggatc aaatgcaaaa cgagacgcgt
     3241 ctgaagcgga tgtcggcggt aattacgtta gttctcggcg cgttgctgct gcttgccgcc
     3301 tggaagccgc cagaaatgat catctggctg aatttgctgg ccttcggtgg gctggaagcc
     3361 gttttcctgt ggccgctggt gctgggtctt tactgggaac gcgccaacgc caaaggcgcg
     3421 ctaagtgcga tgatcgttgg cggcgtgctg tatgccgtac tcgcgacgct gaatattcag
     3481 tacctgggct tccaccctat tgtgccctcg ttactactaa gtttgctggc tttcctggtc
     3541 ggaaaccgtt tcggtacatc cgtcccgcaa gctaccgttt tgactactga taaataaaga
     3601 gttttgccat gccttggatc caactgaaac tgaacaccac cggcgcgaac gcggaagatc
     3661 ttagcgatgc gctgatggaa gcgggtgccg tttctatcac ttttcaggat acccacgata
     3721 cgccagtatt tgagccgctg ccgggcgaaa cgcgcctgtg gggcgacacc gatgtgattg
     3781 gtctgttcga cgctgaaacc gatatgaacg acgtggtggc gattctggaa aaccatccgc
     3841 tgctcggcgc aggcttcgcg cataaaatcg aacaactaga agataaagac tgggagcgcg
     3901 aatggatgga taatttccac ccgatgcgct ttggtgaacg actgtggatc tgccctagct
     3961 ggcgtgatgt gccggacgaa aacgccgtca acgtgatgtt agatccaggg ctggcgtttg
     4021 gtacgggtac ccatccaacc acctctctgt gcctgcaatg gctcgacagc ctcgatttaa
     4081 ccggtaaaac agtcatcgac tttggctgtg gttccggcat tctggcgatc gcggcgctga
     4141 aactgggtgc agcaaaagcc attggtattg atatcgatcc gcaggcgatt caggccagcc
     4201 gcgacaacgc cgaacgtaat ggcgtttctg accgtctgga actctactta ccgaaagatc
     4261 agccagaaga aatgaaagcc gacgtggtgg tcgctaacat ccttgcaggc ccattacgtg
     4321 aactggcacc gttaatcagc gtcctgccgg tttcaggcgg tttgctgggc ctttccggta
     4381 ttctggcaag ccaggcagag agcgtttgtg aagcttatgc cgatagcttc gcactggacc
     4441 cggtcgtgga aaaagaagag tggtgccgta ttaccggtcg taagaattaa ccttcgcatc
     4501 gccgtagggt gacgcggggc gaagtgcgag caagctcaca aaaggcacgt aaatttgccg
     4561 gttatttacg caaatttgcg tgccaaaatt ttcattcata aagaaaaatt gagaacttac
     4621 tcaaatttct ttgagtgtaa attttagtca ctattttcta atatgatgat ttttatgagt
     4681 aattatcgca ccacgctcat tttaaatgca attctttgat ccatctcaga ggattggtca
     4741 aagtttggcc tttcatctcg tgcaaaaaat gcgtaatata cgccgccttg cagtcacagt
     4801 atggtcattt cttaactcat gcgcatcgga caatatcagc tcagaaatcg cctgatcgca
     4861 gcgcccatgg ctggcattac agacagacct tttcggacgt tgtgctacga gatgggagcc
     4921 ggattgacag tatccgagat gatgtcttct aacccacagg tttgggaaag cgacaaatct
     4981 cgtttacgga tggtgcacat tgatgaaccc ggtattcgca ccgtgcaaat tgctggtagc
     5041 gatccgaaag aaatggcaga tgcagcacgt attaacgtgg aaagcggtgc ccagattatt
     5101 gatatcaata tgggttgccc ggctaaaaaa gtgaatcgca agctcgcagg ttcagccctc
     5161 ttgcagtacc cggatgtcgt taaatcgatc cttaccgagg tcgtcaatgc agtggacgtt
     5221 cctgttaccc tgaagattcg caccggctgg gcaccggaac accgtaactg cgaagagatt
     5281 gcccaactgg ctgaagactg tggcattcag gctctgacca ttcatggccg tacacgcgcc
     5341 tgtttgttca atggagaagc tgagtacgac agtattcggg cagttaagca gaaagtttcc
     5401 attccggtta tcgcgaatgg cgacattact gacccgctta aagccagagc tgtgctcgac
     5461 tatacagggg cggatgccct gatgataggc cgcgcagctc agggaagacc ctggatcttt
     5521 cgggaaatcc agcattatct ggacactggg gagttgctgc ccccgctgcc tttggcagag
     5581 gttaagcgct tgctttgcgc gcacgttcgg gaactgcatg acttttatgg tccggcaaaa
     5641 gggtaccgaa ttgcacgtaa acacgtttcc tggtatctcc aggaacacgc tccaaatgac
     5701 cagtttcggc gcacattcaa cgccattgag gatgccagcg aacagctgga ggcgttggag
     5761 gcatacttcg aaaattttgc gtaaacagaa ataaagagct gacagaacta tgttcgaaca
     5821 acgcgtaaat tctgacgtac tgaccgtttc taccgttaac tctcaggatc aggtaaccca
     5881 aaaacccctg cgtgactcgg ttaaacaggc actgaagaac tattttgctc aactgaatgg
     5941 tcaggatgtg aatgacctct atgagctggt actggctgaa gtagaacagc ccctgttgga
     6001 catggtgatg caatacaccc gtggtaacca gacccgtgct gcgctgatga tgggcatcaa
     6061 ccgtggtacg ctgcgtaaaa aattgaaaaa atacggcatg aactaattca ggttagctaa
     6121 atgcttgatt aaaaaggcgc tactcggcat ggggaagcgc cttttttata ggtgtcacaa
     6181 agggagtgac catgagaaca ggatgtgaac cgacccggtt tggtaatgaa gctaagacca
     6241 ttattcacgg tgatgcgctt gccgaactta aaaagatccc cgccgaaagt gtcgatctga
     6301 tctttgccga cccaccatat aacatcggta aaaattttga tggtctgatc gaagcctgga
     6361 aagaagatct gtttatcgac tggctgtttg aagtgattgc agagtgccac cgcgttctga
     6421 aaaagcaggg cagcatgtac atcatgaaca gcacggaaaa catgcccttt atcgatctcc
     6481 agtgccgcaa gctttttacc atcaaaagtc gcatcgtctg gtcatatgac agttctggag
     6541 tacaggcgaa aaaacactac ggctccatgt acgaacccat cctgatgatg gtgaaagacg
     6601 caaagaacta cacattcaac ggtgatgcta ttctggtaga agccaaaacc ggatcgcagc
     6661 gcgcgttgat cgattatcgc aaaaatcctc cacagccata caatcatcaa aaagtaccgg
     6721 gtaacgtctg ggattttccg cgcgtgcgtt atttgatgga tgaatatgag aaccacccga
     6781 cgcaaaaacc ggaagcctta ctgaaacgca ttattctcgc ctcttccaac ccaggcgata
     6841 tcgttctcga cccgtttgcc ggtagcttta ctaccggtgc cgtagccgtc tccagcgggc
     6901 gaaaattcat tggtattgag atcaacagcg agtacatcaa aatggggctt cgacggctgg
     6961 atgtcgcgtc gcattactct gcggaagaac tggcgaaagt gaaaaaaaga aagaccggca
     7021 acctgtcaaa acgaagccgg ttaagcgaag ttgaccccga tctcattgca aagtaaagga
     7081 agtgtaagcc tggttttcag attattcatt tcgtgtatat ttcctgccag acttggttaa
     7141 acatacacag gctcaggtaa tgattcgcaa gtattggtgg ctcgtcgttt tcgctgtctt
     7201 cgtttttctg tttgatactt tactgatgca gtggattgaa ctgctggcaa cagaaacaga
     7261 caaatgccgc aatatgaact cagttaatcc actaaaactg gttaactgtg acgaactgaa
     7321 ttttcaggac agaatgtgaa tttacatgac acttaattca ttc
//
LOCUS       DAZKHF020000211         7305 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2201S31241
            SAMN43267800-rid25106413.denovo.213, whole genome shotgun sequence.
ACCESSION   DAZKHF020000211 DAZKHF020000000
VERSION     DAZKHF020000211.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267800
            Sequence Read Archive: SRR30307263
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 7305)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7305)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 7305)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350581.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350581.2
            Genome Coverage       :: 151x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:21:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,652
            CDSs (total)                      :: 5,557
            Genes (coding)                    :: 5,320
            CDSs (with protein)               :: 5,320
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 237
            CDSs (without protein)            :: 237
            Pseudo Genes (ambiguous residues) :: 0 of 237
            Pseudo Genes (frameshifted)       :: 102 of 237
            Pseudo Genes (incomplete)         :: 130 of 237
            Pseudo Genes (internal stop)      :: 52 of 237
            Pseudo Genes (multiple problems)  :: 41 of 237
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7305
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267800-rid25106413.denovo.213"
                     /serotype="O 26:H11"
                     /isolate="2201S31241"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-01"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            <1..1638
                     /locus_tag="ACHOSC_RS18550"
                     /old_locus_tag="pgaptmp_003710"
     CDS             <1..1638
                     /locus_tag="ACHOSC_RS18550"
                     /old_locus_tag="pgaptmp_003710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020233613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate porin"
                     /protein_id="HGU4549566.1"
                     /translation="FSIITALFPLNAMATKLTIEQRLELLENELSQNKQELKATQNEL
                     GVYKSRLSTLQKSITENKYKSASLAEISATSPVADNIKNENGEQNSFTAAHTINGSQQ
                     VAVIESKGDKTTIESVTLKDISKYIKDDIGFSYQGYFRSGWGTGNHGSPQTYAAGSLG
                     RFGNEMSGWFDLTLNQRVYNQDGKTANAVVTYDGNVGEQYNDAWFGDSANENIMQFSD
                     IYLTTRGFLPFAPEADFWVGKHKLPQYEIQMLDWKTLTTDVAAGVGIENWALGVGLFD
                     MSLSRDDVDVYSRDFSRTSQMNTNSVDVRYRNIPLWDDATLSLMAKYSAPNKTDQQQD
                     NENDNSYFEMKDSWMLTSVLRQNLQRDTFNEFTLQVANNSYASSFASFSDASNTMAHG
                     RYYYGDHTNGIAWRLISQGEMYLTDNIIMANALVYSHGEDVYSYESGAHSDFDSIRTV
                     IRPAWIWNTWNQTGLELGWFKQQNKTQQGVTLNESAYKTTLWHALKVGESILGSRPEI
                     RFYGTYINILDNELSNFKFNENSKDEFMAGIQAEVWW"
     gene            1663..3102
                     /locus_tag="ACHOSC_RS18555"
                     /old_locus_tag="pgaptmp_003711"
     CDS             1663..3102
                     /locus_tag="ACHOSC_RS18555"
                     /old_locus_tag="pgaptmp_003711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000012594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase"
                     /protein_id="HGU4549567.1"
                     /translation="MSGFKKGFLWGGAVAAHQLEGGWNEGGKGISIADVMTAGAHGVP
                     REVTEGVIDGLNYPNHEAIDFYHRYKTDIQLFAEMGFKCFRTSIAWTRIFPQGDEQEP
                     NEEGLQFYDDLFDECLKQGMEPVVTLSHFEMPYHLVTKYGGWRNRKLIDFFIRFASTV
                     FTRYKEKVKYWMTFNEINNQVNFSESLCPFTNSGILYSPEEDINEREQIMYQAVHYEL
                     VASALAVQTGKSINPEFNIGCMIAMCPIYPLTCAPNDMMMATKAMHRRYWFTDVHARG
                     YYPQHMLNYFARKGFNLDITPEDNAILASGCVDFIGFSYYMSFTTQFSPDNPQLDYVE
                     PRDLVSNPYIDTSEWGWQIDPAGLRYSLNWFWDHFQLPLFIVENGFGAVDQRQADGTV
                     NDHYRIDYFASHIREMKKAVVEDGVDLIGYTPWGCIDLVSAGTGEMKKRYGMIYVDKD
                     NEGKGTLERIRKASFYWYRDLIANNGENI"
     gene            complement(3159..3377)
                     /gene="marB"
                     /locus_tag="ACHOSC_RS18560"
                     /old_locus_tag="pgaptmp_003712"
     CDS             complement(3159..3377)
                     /gene="marB"
                     /locus_tag="ACHOSC_RS18560"
                     /old_locus_tag="pgaptmp_003712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multiple antibiotic resistance protein MarB"
                     /protein_id="HGU4549568.1"
                     /translation="MKPLSSAIAAALILFSAQGVAEQTTQPVVTSCANVVVVPPSQEQ
                     PPFDLNHMGTGSDKSDALGVPYYNQHAM"
     gene            complement(3409..3792)
                     /gene="marA"
                     /locus_tag="ACHOSC_RS18565"
                     /old_locus_tag="pgaptmp_003713"
     CDS             complement(3409..3792)
                     /gene="marA"
                     /locus_tag="ACHOSC_RS18565"
                     /old_locus_tag="pgaptmp_003713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707445.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MDR efflux pump AcrAB transcriptional activator
                     MarA"
                     /protein_id="HGU4549569.1"
                     /translation="MSRRNTDAITIHSILDWIEDNLESPLSLEKVSERSGYSKWHLQR
                     MFKKETGHSLGQYIRSRKMTEIAQKLKESNEPILYLAERYGFESQQTLTRTFKNYFDV
                     PPHKYRMTNMQGESRFLHPLNHYNS"
     gene            complement(3812..4246)
                     /gene="marR"
                     /locus_tag="ACHOSC_RS18570"
                     /old_locus_tag="pgaptmp_003714"
     CDS             complement(3812..4246)
                     /gene="marR"
                     /locus_tag="ACHOSC_RS18570"
                     /old_locus_tag="pgaptmp_003714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310164.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multiple antibiotic resistance transcriptional
                     regulator MarR"
                     /protein_id="HGU4549570.1"
                     /translation="MKSTSDLFNEIIPLGRLIHMVNQKKDRLLNEYLSPLDITAAQFK
                     VLCSIRCAACITPVELKKVLSVDLGALTRMLDRLVCKGWVERLPNPNDKRGVLVKLTT
                     SGAAICEQCHQLVGQDLHQELTKNLTADEVATLEHLLKKVLP"
     gene            4458..5123
                     /gene="marC"
                     /locus_tag="ACHOSC_RS18575"
                     /old_locus_tag="pgaptmp_003715"
     CDS             4458..5123
                     /gene="marC"
                     /locus_tag="ACHOSC_RS18575"
                     /old_locus_tag="pgaptmp_003715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAAT family transporter MarC"
                     /protein_id="HGU4549571.1"
                     /translation="MLDLFKAIGLGLVVLLPLANPLTTVALFLGLAGNMNSAERNRQS
                     LMASVYVFAIMMVAYYAGQLVMDTFGISIPGLRIAGGLIVAFIGFRMLFPQQKAIDSP
                     EAKSKSEELEDEPSANIAFVPLAMPSTAGPGTIAMIISSASTVRQSSTFADWVLMVAP
                     PLIFFLVAVILWGSLRSSGAIMRLVGKGGIEAISRLMGFLLVCMGVQFIINGILEIIK
                     TYH"
     gene            complement(5148..6338)
                     /gene="ydeA"
                     /locus_tag="ACHOSC_RS18580"
                     /old_locus_tag="pgaptmp_003716"
     CDS             complement(5148..6338)
                     /gene="ydeA"
                     /locus_tag="ACHOSC_RS18580"
                     /old_locus_tag="pgaptmp_003716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-arabinose MFS transporter"
                     /protein_id="HGU4549572.1"
                     /translation="MTTNTVSRKVAWLRVVTLAVAAFIFNTTEFVPVGLLSDIAQSFH
                     MQTAQVGIMLTIYAWVVALMSLPFMLMTSQVERRKLLICLFVVFIASHVLSFLSWSFT
                     VLVISRIGVAFAHAIFWSITASLAIRMAPAGKRAQALSLIATGTALAMVLGLPLGRIV
                     GQYFGWRMTFFAIGIGALITLLCLIKLLPLLPSEHSGSLKSLPLLFRRPALMSIYLLT
                     VVVVTAHYTAYSYIEPFVQNIAGFSANFATALLLLLGGAGIIGSVIFGKLGNQYASAL
                     VSTAIALLLVCLALLLPAANSEIHLGVLSIFWGIAMMIIGLGMQVKVLALAPDATDVA
                     MALFSGIFNIGIGAGALVGNQVSLHWSMSMIGYVGAVPAFAALIWSIIIFRRWPVTLE
                     EQTQ"
     gene            complement(6488..7299)
                     /gene="yneK"
                     /locus_tag="ACHOSC_RS18585"
                     /old_locus_tag="pgaptmp_003717"
                     /pseudo
     CDS             complement(6488..7299)
                     /gene="yneK"
                     /locus_tag="ACHOSC_RS18585"
                     /old_locus_tag="pgaptmp_003717"
                     /inference="COORDINATES: protein motif:HMM:NF033404.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="putative protein YneK"
BASE COUNT         2037 a         1574 c         1769 g         1925 t
ORIGIN      
        1 ttttctatta ttacagcatt gtttccattg aacgcgatgg caacaaaatt aaccatagag
       61 cagcgccttg aattgcttga aaatgaattg tcgcaaaata aacaagagct gaaagcaaca
      121 cagaatgaac tgggagtata taaatcccga ctttcgacct tacaaaaaag catcacggaa
      181 aacaaatata aatcagcctc gcttgctgaa atatcagcga catctcccgt cgctgataac
      241 atcaaaaatg aaaacggtga acagaactcg tttaccgcag cgcatactat aaatggatcg
      301 cagcaagttg ccgttattga aagtaaaggc gacaaaacca ctatcgaaag cgtgaccctg
      361 aaagatatca gtaaatatat aaaggatgat attgggttca gctatcaggg gtacttccgc
      421 tcaggctggg gtactggtaa tcacggctca ccacaaactt atgcagcggg ttctctggga
      481 cgttttggta atgagatgag cggttggttt gacctgacct taaatcagcg tgtttataat
      541 caggacggta aaacggcaaa tgcggtcgtt acctatgatg gcaacgtggg tgagcagtat
      601 aacgatgcct ggtttggtga cagtgccaat gaaaatatca tgcagttcag cgatatttac
      661 ctgacaacgc gaggtttttt acccttcgca ccagaggcag acttctgggt cggcaaacat
      721 aaactcccgc aatatgagat ccagatgctg gactggaaaa ccttaaccac ggatgtcgcc
      781 gcgggtgtgg ggattgaaaa ctgggcactt ggtgtagggc tgtttgatat gtccttaagc
      841 cgtgatgatg tcgatgttta ctctcgtgat ttttcgcgta ccagccagat gaataccaat
      901 tctgtggatg ttcgttatcg caatatcccg ttatgggatg atgcgacatt atcattaatg
      961 gctaaatatt ccgcacctaa taaaacggat caacaacaag ataacgaaaa tgacaacagt
     1021 tattttgaaa tgaaagatag ctggatgctg acttctgttt tacggcaaaa cctgcaacgc
     1081 gatacgttta atgaatttac gttacaggtt gccaataatt cctatgccag cagttttgcc
     1141 agtttctcag atgccagtaa tacgatggcg catggtcgct attactatgg tgatcatacc
     1201 aatgggatcg cctggcgttt aatctctcag ggcgagatgt atcttactga caatattatt
     1261 atggctaacg cgcttgtcta ttctcacggc gaagatgttt atagttatga aagtggtgct
     1321 catagtgatt ttgacagtat tcgcactgta ataagaccgg cctggatctg gaatacatgg
     1381 aatcagacgg ggcttgaatt aggctggttt aagcaacaga acaaaactca gcagggtgta
     1441 acactaaatg aatcggctta taaaacgaca ctctggcatg cattgaaagt gggtgaaagc
     1501 attttaggtt cacgaccaga aattcgcttc tatggaacgt atatcaatat tctggataac
     1561 gaattatcga attttaagtt taatgagaac agcaaagacg agtttatggc cggcatccag
     1621 gcggaagtct ggtggtaatc tattttagtt aaggagttgt gtatgtcagg atttaaaaaa
     1681 ggttttttat ggggcggtgc ggtagccgcg catcagttgg aaggtggctg gaatgaagga
     1741 ggaaaaggca tcagcatcgc tgatgtgatg actgctggcg ctcacggggt gccgcgtgaa
     1801 gtgacagaag gcgttatcga cgggcttaat tatcccaatc atgaagcaat tgatttttat
     1861 catcgctata aaacagatat tcagttattt gccgagatgg gattcaaatg ctttcgaact
     1921 tccattgcct ggacacgaat ctttccgcaa ggtgacgaac aggagccgaa tgaagagggt
     1981 ttacaatttt atgatgatct gttcgatgaa tgccttaagc agggaatgga acctgtggtg
     2041 acgctttcac attttgagat gccttatcat ctggtgacaa aatatggtgg ctggcgaaac
     2101 cgtaaactga tcgacttttt tatccgcttc gcatcaacgg tcttcacgcg ctataaagaa
     2161 aaagtaaagt actggatgac gtttaacgaa atcaataatc aggtgaattt cagcgaaagc
     2221 ctgtgtccat ttactaattc cggtatcttg tattcgccag aggaagatat caatgagcgc
     2281 gaacaaataa tgtaccaggc ggtacattac gagttagttg ccagtgccct ggcggtacag
     2341 actggaaaat cgatcaatcc tgaatttaac attggctgta tgatcgccat gtgtcccatc
     2401 tatcctctga cgtgtgcacc caacgatatg atgatggcca cgaaagcaat gcatcgtcgt
     2461 tactggttta ctgatgttca tgctcgtgga tattatccgc aacatatgct gaattacttt
     2521 gccaggaaag gattcaacct cgatatcaca ccagaagata acgcgattct tgccagtggt
     2581 tgtgtcgact ttatcggctt tagctactac atgtctttta cgacacaatt ttcgcccgat
     2641 aacccgcaac tggattatgt tgaaccacga gatttggtca gcaaccctta tatcgataca
     2701 tccgaatggg gatggcaaat tgatccggca gggctacgtt attcactcaa ctggttctgg
     2761 gatcatttcc agttgccgct gtttattgtc gaaaatggat ttggtgcggt tgaccagaga
     2821 caagctgacg gcacggtgaa cgatcactat cgcattgatt actttgcttc ccatattcgg
     2881 gaaatgaaaa aagccgtagt tgaagatggt gttgacttaa ttggctacac cccgtggggc
     2941 tgcattgacc tggtttctgc cggaacaggg gaaatgaaaa agcgctacgg aatgatttat
     3001 gtcgataaag acaacgaagg gaagggaacg ctggaacgga tacgtaaagc gtcgttttac
     3061 tggtatcggg atctcatcgc caacaatggc gaaaatattt gagacttgag gaagatatta
     3121 cggagatgtc aggctctgtg atcagagcct gacaataact acatagcgtg ttgattataa
     3181 tagggcacgc cgagcgcatc cgacttatca ctgccagtac ccatgtgatt taaatcaaac
     3241 ggtggttgtt cctgcgatgg gggaacaacc acgacattgg cacaagaagt aacaactggc
     3301 tgcgtggttt gttccgcaac gccctgcgcg gaaaagagaa taagcgcagc tgctattgcg
     3361 gatgaaagtg gtttcatgat tgcctcagtg acgttgtcac gttatcaact agctgttgta
     3421 atgatttaat ggatgtaaaa agcgcgattc gccctgcata ttggtcatcc ggtatttatg
     3481 cggcggaaca tcaaagtaat ttttgaaggt tcgggtcaga gtttgttgcg actcgaagcc
     3541 atatcgttct gccagataga gtatcggctc gttactttcc ttcagctttt gcgcgatttc
     3601 cgtcatctta cggctgcgga tgtattggcc taatgaatga ccggtttctt ttttaaacat
     3661 ccgttgcagg tgccatttag agtaacctga acgctctgac actttctcca gtgacaatgg
     3721 cgattccagg ttgtcctcga tccagtccaa aatgctatga atggtaatag cgtcagtatt
     3781 gcgtctggac atcgtcatac ctcttttttg tttacggcag gactttctta agcaaatgct
     3841 caagtgttgc cacttcgtcc gccgtcaggt tttttgttaa ttcttgatgc aggtcctggc
     3901 caactaattg atggcattgt tcgcatattg ccgcgccgct ggtggtaagt tttaccagta
     3961 cgccgcgctt atcattcggg ttcggcaacc tttcaaccca gcctttacag accaggcgat
     4021 ccagcatacg ggtcagtgct ccaaggtcga cagacaacac ttttttcagt tcaaccggag
     4081 taatacacgc cgcgcagcgg atagagcaga gcaccttaaa ctgtgccgcg gtaatatcca
     4141 gcggagacag atactcgtta agcagacgat ctttcttctg attaaccata tggattaagc
     4201 gacccaatgg aataatttca ttgaacagat cgctggtact tttcacatta gttgccctgg
     4261 caagtaatta gttgcagggg ataatattgc ccaggcaagt ataagtcaaa taaatgaatt
     4321 gaccgatgcc acgttttgct aaatcggttc aagttttggt caatgcgatg caaggttagc
     4381 taaagagtga cactttatcc ttgtttaccc cttatacttt tcgctgataa cccagataca
     4441 caggataaca accaccaatg ttagatttgt ttaaagcaat tggcctgggg ctggtggtgt
     4501 tactgccgtt agctaaccca ttaacaactg tcgcgttatt tcttggcctg gcaggcaaca
     4561 tgaacagtgc cgaacgtaat cgtcagtcgt tgatggcctc ggtgtacgta tttgccatca
     4621 tgatggtggc gtattacgcc gggcaactgg tgatggatac atttggcatt tcgattcccg
     4681 gtctgcgaat tgcaggcggc ttaatcgtcg cgtttatcgg ttttcggatg ctctttccgc
     4741 aacagaaagc gattgattca ccggaggcga aaagcaagtc ggaagagctg gaagatgaac
     4801 ccagtgccaa tattgccttt gtgccgctag caatgccaag taccgctggt ccgggaacca
     4861 tagcgatgat tattagctcg gcgtccacag tgcgtcagag ttcaaccttc gctgattggg
     4921 tactgatggt tgcgccgccg ctgatctttt tcctggtggc ggttattttg tggggaagcc
     4981 tacgcagctc cggcgcaatt atgcggctgg tgggcaaggg tggcattgaa gcgatctccc
     5041 gtctgatggg attcctgctg gtatgtatgg gtgtgcagtt tattattaac ggcatcctgg
     5101 aaatcattaa aacgtaccat taaaaaaggc ccgaatgggc ctttcaacta ttgcgtctgt
     5161 tcttcgagtg tcactggcca gcggcgaaat ataatgattg accaaattaa cgcggcaaaa
     5221 gcaggcaccg cgcccacata accaatcatc gacattgacc agtgcaaact cacctgatta
     5281 cctaccaacg cacccgcccc gattccaata ttaaatatcc cggagaatag cgccatcgcg
     5341 acgtcggtag catctggtgc cagcgccagc actttaacct gcataccaag cccgatgatc
     5401 atcatcgcga tcccccagaa aatactcagc accccgaggt gtatttcact gttcgccgca
     5461 ggtaacagca atgccaggca caccagcaac agcgcaatcg ccgtactcac caacgcagac
     5521 gcatactgat tacccagttt accgaaaatc acgctgccaa taatgcccgc accaccgagt
     5581 aataacagta atgccgtggc aaagttggcg ctgaatcccg caatgttttg tacaaaaggc
     5641 tcgatatagc tgtatgccgt gtaatgggcg gtgacaacca ccacagttaa caaataaatg
     5701 ctcatcaatg ccgggcggcg gaacaatagc gggaggcttt tcagtgaacc ggaatgctca
     5761 ctgggcagta agggaagtaa cttaatcagg cacaaaaggg tgataagcgc cccaatacca
     5821 atcgcgaaga aggtcattcg ccaaccgaaa tactggccca caatgcgccc gagaggtaaa
     5881 cctaagacca tcgccagtgc tgtaccggtg gcaattaaac tcaatgcctg tgctcgcttc
     5941 ccggccggag ccatacggat cgccagagac gccgtaatcg accagaaaat cgcatgtgca
     6001 aaagccacac caatgcgact gatcaccaga acggtaaagc tccacgataa aaacgacagt
     6061 acgtggctgg caataaacac cacaaacagg cagatcagta atttgcgccg ttcaacctgg
     6121 ctggtcatta acataaaagg caatgacatt agcgccacta cccatgcata aatggtcaac
     6181 ataatgccga cctgagcggt ttgcatgtga aaactttgcg caatgtcaga gagcaggcca
     6241 acagggacaa attcggtggt gttgaagatg aaggcggcga ctgccagcgt aacgacccgt
     6301 agccacgcca ctttgcggga aacagtgttt gttgtcataa cagattatcg ggtgagttgc
     6361 gaaaaagtga tgaaatgatc ttaaaacggt taatgatgtg aatacaatgc atttgtgatc
     6421 tggatcgcgt tttccgtttc tctgacgctg attctgttcc gcgtcagaga gtcggtaaca
     6481 tcacggttta atgacgttca gggaggtctg gcgttttggc ggttgcgggt ataggtggtc
     6541 tgacagggga gcgaggcctg tttttcacca ggtcttcgtg agtaactttt ggcggcgttt
     6601 cattttttac aaacagtttg agggtcctgc gcgccatatg tttgataatt ttatcatcac
     6661 ttaatgatgc tggcatcaac acgtcattca gatcactttc attgggattc ataaattggt
     6721 gatagtgcaa taacatattg gcgaaaaata aaaaaaagtc ctgaaaatga ggagattctt
     6781 gttctgctaa aagggataaa aagcagatgc agacttcggg tagtacatgt ttgttagaac
     6841 ctaatgaatc catcaggctt tgcaataaat cctcttttaa ttcgccgtct gttatgtcga
     6901 gaatggcggt gatcaagtta ggatctttat tgacgaacca tctgtgaacc tcagagttac
     6961 tccataatcg ggggagtatg acaaaattac cgcgagagat aaaattcagg acaagcaaat
     7021 caaactggcg taagtaatct gcagcggtat ggcgatccgg aggcaggggt tcaatatgtg
     7081 gtgtatgcgc tcttttgatg ggaacgccaa ggttgatcat tggaaattgt tcaataaaat
     7141 cattcagaaa cagaaatagc acacgctcga cgccggtctg aaatacgcgc cccatagtga
     7201 caggctttgg gttgggagcg caaaaatatt aatttctcca tcatggcgaa aattatctaa
     7261 ataaataggg cttaaattta ttactacctg aaattcaata ttttc
//
LOCUS       DAZKHF020000212         7299 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2201S31241
            SAMN43267800-rid25106413.denovo.214, whole genome shotgun sequence.
ACCESSION   DAZKHF020000212 DAZKHF020000000
VERSION     DAZKHF020000212.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267800
            Sequence Read Archive: SRR30307263
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 7299)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7299)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 7299)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350581.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350581.2
            Genome Coverage       :: 151x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:21:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,652
            CDSs (total)                      :: 5,557
            Genes (coding)                    :: 5,320
            CDSs (with protein)               :: 5,320
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 237
            CDSs (without protein)            :: 237
            Pseudo Genes (ambiguous residues) :: 0 of 237
            Pseudo Genes (frameshifted)       :: 102 of 237
            Pseudo Genes (incomplete)         :: 130 of 237
            Pseudo Genes (internal stop)      :: 52 of 237
            Pseudo Genes (multiple problems)  :: 41 of 237
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7299
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267800-rid25106413.denovo.214"
                     /serotype="O 26:H11"
                     /isolate="2201S31241"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-01"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            <1..419
                     /gene="ecpR"
                     /locus_tag="ACHOSC_RS18590"
                     /old_locus_tag="pgaptmp_003718"
                     /gene_synonym="matA"
     CDS             <1..419
                     /gene="ecpR"
                     /locus_tag="ACHOSC_RS18590"
                     /old_locus_tag="pgaptmp_003718"
                     /gene_synonym="matA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ECP biosynthesis operon DNA-binding
                     transcriptional regulator EcpR"
                     /protein_id="HGU4549573.1"
                     /translation="KDFVFINLNTDSLSEFINRDNEWLSAVKGKQVVLIAARKSEALA
                     NYWYYNSNIRGVVYAGLSRDIRKELAYVINGRFLRKDIKKDKITDREMEIIRMTAQGM
                     QPKSIARIENCSVKTVYTHRRNAEAKLYSKIYKLVQ"
     gene            493..1080
                     /gene="ecpA"
                     /locus_tag="ACHOSC_RS18595"
                     /old_locus_tag="pgaptmp_003719"
                     /gene_synonym="matB"
     CDS             493..1080
                     /gene="ecpA"
                     /locus_tag="ACHOSC_RS18595"
                     /old_locus_tag="pgaptmp_003719"
                     /gene_synonym="matB"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="common pilus major fimbrillin subunit EcpA"
                     /protein_id="HGU4549574.1"
                     /translation="MKKKVLAIALVTVFTGIGVAQAADVTAQAVATWSATAKKDTTSK
                     LVVTPLGSLAFQYAEGIKGFNSQKGLFDVAIEGDSTATAFKLTSRLITNTLTQLDTSG
                     STLNVGVDYNGAAVEKTGDTVMIDTANGVLGGNLSPLANGYNASNRTTAQDGFTFSII
                     SGTTNGTTAVTDYSTLPEGIWSGDVSVQFDATWTS"
     gene            1138..1806
                     /gene="ecpB"
                     /locus_tag="ACHOSC_RS18600"
                     /old_locus_tag="pgaptmp_003720"
                     /gene_synonym="yagY"
     CDS             1138..1806
                     /gene="ecpB"
                     /locus_tag="ACHOSC_RS18600"
                     /old_locus_tag="pgaptmp_003720"
                     /gene_synonym="yagY"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial chaperone EcpB"
                     /protein_id="HGU4549575.1"
                     /translation="MKKHLLPLALLFSGISPAQALDVGDISSFMNSDSSTLSKTIKNS
                     TDSGRLINIRLERLSSPLDDGQVISMDKPDELLLTPASLLLPAQASEVIRFFYKGPAD
                     EKERYYRIVWFDQALSDAQRDNANRSAVATASARIGTILVVAPRQANYHFQYANGSLT
                     NTGNATLRILAYGPCLKAANGKECKENYYLMPGKSRRFTRVDTADNKGRVALWQGDKF
                     IPVK"
     gene            1832..4357
                     /gene="ecpC"
                     /locus_tag="ACHOSC_RS18605"
                     /old_locus_tag="pgaptmp_003721"
                     /gene_synonym="yagX"
     CDS             1832..4357
                     /gene="ecpC"
                     /locus_tag="ACHOSC_RS18605"
                     /old_locus_tag="pgaptmp_003721"
                     /gene_synonym="yagX"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial usher EcpC"
                     /protein_id="HGU4549576.1"
                     /translation="MPLRRFSPGLKAQFAFGMVFLFVQPDASAADISAQQIGGVIIPQ
                     AFSQALQDGMSVPLYIHLAGSQGRQDDQRIGSAFIWLDDGQLRIRKIQLEESEDNASV
                     SEQTRQQLMALANAPFNEALTIPLTDNAQLDLSLRQLLLQLVVKREALGTVLRSRSED
                     IGQSSVNTLSSNLSYNLGVYNNQLRNGGSNTSSYLSLNNVTALREHHVVLDGSLYGIG
                     SGQQDSELYKAMYERDFAGHRFAGGMLDTWNLQSLGPMTAISAGKIYGLSWGNQASST
                     IFDSSQSATPVIAFLPAAGEVHLTRDGRLLSVQNFTMGNHEVDTRGLPYGIYDVEVEV
                     IVNGRVISKRPQRVNKLFSRGRGVGAPLAWQVWGGSFHMDRWSENGKKTRPAKESWLA
                     GASTSGSLSTLSWAATGYGYDNQAVGETRLTLPLGGAINVNLQNMLASDSSWSSIGSI
                     STTLPGGFSSLWVNQEKTRIGNQLRRSDADNRAIGGTLNLNSLWSKLGTFSISYNDDR
                     RYNSHYYTADYYQNVYSGTFGSLGLRAGIQRYNNGDSNANTGKYIALDLSLPLGNWFS
                     AGMTHQNGYTMANLSARKQFDEGTIRTVGANLSRAISGDTGDDKTLSGGAYAQFDARY
                     ASGTLNVNSAADGYINTNLTANGSVGWQGKNIAASGRTDGNAGVIFNTGLEDDGQISA
                     KINGRIFPLNGKRNYLPLSPYGRYEVELQNSKNSLDSYDIVSGRKSHLTLYPGNVAVI
                     EPEVKQMVTVSGRIRAEDGTLLANARINNHIGRTRTDENGEFVMDVDKKYPTIDFRYS
                     GNKTCEVALELNQARGAVWVGDVVCSGLSSWAEVTQTGEENES"
     gene            4347..5990
                     /gene="ecpD"
                     /locus_tag="ACHOSC_RS18610"
                     /old_locus_tag="pgaptmp_003722"
     CDS             4347..5990
                     /gene="ecpD"
                     /locus_tag="ACHOSC_RS18610"
                     /old_locus_tag="pgaptmp_003722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial adhesin EcpD"
                     /protein_id="HGU4549577.1"
                     /translation="MRVNLLITMIIFALIWPVTELRAAVSKTTWADAPAREFVFVENN
                     SDDNFFVTPGGALDPRLTGANRWTGLKYTGSGTIYQQSLGYIDNGYNTGLYTNWKFDM
                     WLENSPVSSPLTGLRCINWYAGCNMTTSLILPQTTDASGFYGATVTSGGAKWMHGMLS
                     DAFYQYLQQMPVGSSFTMTINACQTSVNYDASSGARCKDQASGNWYVRNVTHTKAANL
                     RLINTHSLAEVFINSDGVPTLGEGNADCRTQTIGSLSGLSCKMVNYTLQTNGLSNTSI
                     HIFPAIANSSLASAVGAYDMQFSLNGSSWKPVSNTAYYYTFNEMKSADSIYVFFSSNF
                     FKQMVNLGISDINTKDLFNFRFQNTTSPESGWYEFSTSNTLIIKPRDFSISIISDEYT
                     QTPSREGYVGSGESALDFGYIVTTSGKTAADEVLIKVTGPAQVIGGRSYCVFSSDDGK
                     AKVPFPATLSFITRNGATKTYDAGCDDSWRDMTDALWLTTPWTDISGEVGQMDKTTVK
                     FSIPMDNAISLRTVDDNGWFGEVSASGEIHVQATWRNIN"
     gene            5959..6669
                     /gene="ecpE"
                     /locus_tag="ACHOSC_RS18615"
                     /old_locus_tag="pgaptmp_003723"
     CDS             5959..6669
                     /gene="ecpE"
                     /locus_tag="ACHOSC_RS18615"
                     /old_locus_tag="pgaptmp_003723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414823.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial chaperone EcpE"
                     /protein_id="HGU4549578.1"
                     /translation="MFRRRGVTLTKALLTAVCMLAAPLTQAISVGNLTFSLPSETDFV
                     SKRVVNNNKSARIYRIAISAIDSPGSSELRTRPVDGELLFAPRQLALQAGESEYFKFY
                     YHGPRDNRERYYRVSFREVPTRNQTRRSPTGGEVSTEPVVVMDTILVVRPRQVQFKWS
                     FDKVTGTVSNTGNTWFKLLIKPGCDSTEEEGDAWYLRPGDVVHQPELRQPGNHYLVYN
                     DKFIKISDSCPAKPPSAD"
     gene            6982..>7299
                     /locus_tag="ACHOSC_RS18620"
                     /old_locus_tag="pgaptmp_003724"
     CDS             6982..>7299
                     /locus_tag="ACHOSC_RS18620"
                     /old_locus_tag="pgaptmp_003724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YkgJ family cysteine cluster protein"
                     /protein_id="HGU4549579.1"
                     /translation="MSNLNPCMTCGACCAFFRVSFYWAEADDAGGTIPARLTEQISPF
                     HRCMSGTNQKNPRCIALAGTPGKNACCTIYKNRSSTCREFAMSGENGVVNEACNRARA
                     KYGL"
BASE COUNT         1868 a         1892 c         1923 g         1616 t
ORIGIN      
        1 gaaaagattt cgtgtttatc aatctcaata cggattcttt atctgaattt ataaaccgtg
       61 ataatgaatg gttatccgcg gtaaagggga aacaggtcgt attgattgcg gccagaaagt
      121 cagaagcctt agcaaattat tggtattaca acagcaatat taggggcgtg gtatacgcag
      181 gactgagtcg tgatattaga aaagaactgg cctatgtgat taatggcagg ttcctgagaa
      241 aagatattaa gaaagataaa atcacggacc gggaaatgga aattatccgc atgacggccc
      301 agggaatgca acctaaatcg attgccagaa ttgaaaattg tagtgtgaag acagtgtata
      361 cccatcggcg taatgctgag gccaagctgt actcaaaaat atataagttg gttcagtaaa
      421 ctccaggcta gttagtttta aaaatgactc actgggacat cacgtcctca attcaactcg
      481 ggaagaaatg caatgaaaaa aaaggttctg gcaatagctc tggtaacggt gtttaccggt
      541 ataggtgtgg cgcaggctgc tgacgtaaca gctcaggctg tagcgacctg gtcagcaaca
      601 gccaaaaaag acaccaccag taagctggtt gtgacgccac tcggtagcct agcgttccag
      661 tatgccgaag gcattaaagg ttttaactca cagaaaggtc tatttgacgt ggctatcgag
      721 ggtgactcaa cggctaccgc ctttaaactg acctcacgtc ttatcaccaa cacattaacc
      781 cagttggata cctcaggttc cacactgaat gtgggcgtgg attataacgg cgcggcagtc
      841 gaaaaaactg gcgataccgt gatgatcgat accgccaacg gcgtactggg cggcaacctt
      901 agcccgctgg caaacggtta caatgccagc aatcgtacca ccgcacagga tggtttcact
      961 ttctccatca tcagcggcac caccaatggt accaccgcag taaccgatta cagcactcta
     1021 ccggaaggca tctggagcgg cgacgttagc gtacagttcg acgcgacctg gaccagttaa
     1081 tctctctgat gtaccagcag gggtagcccc cctgctttat tcctggacgg aattgttatg
     1141 aaaaagcacc ttctgcctct cgctctgctg ttttccggaa tatctccggc ccaggcgctg
     1201 gatgtcggcg atatatcatc gtttatgaac agtgacagca gcacgctgag caaaacgatc
     1261 aaaaacagta ccgacagtgg tcgccttatc aatatccgtc tcgaacggct ctcttcaccg
     1321 cttgacgacg ggcaggttat ctcaatggac aagccggatg agttgctact cactcccgcc
     1381 agcttgctgc tacccgccca agccagcgaa gtgatccgct tcttctataa gggacccgca
     1441 gatgaaaaag agcgctacta ccgcattgtc tggtttgatc aggccctcag tgatgcgcag
     1501 cgcgataatg ccaaccgcag cgctgtggcc actgcttccg cccgcatcgg caccattctg
     1561 gtcgtcgccc ctcgtcaggc gaactaccac tttcagtacg ccaacggctc cctgacaaat
     1621 acaggaaatg cgacgctgcg gatcctcgct tacggcccct gcctgaaagc cgccaatggt
     1681 aaggagtgta aagagaatta ctacctgatg ccaggcaagt cgcgtcgttt tacccgcgtg
     1741 gacacggcgg ataacaaagg acgggttgca ctttggcagg gtgataagtt cattcccgtg
     1801 aaatagatag ctgtgcagat ggataacgac aatgccttta cgacggttct ccccaggact
     1861 gaaagcccag tttgccttcg gcatggtctt tttgttcgtt caacccgatg ccagcgctgc
     1921 tgacataagt gcgcagcaaa taggtggggt gattattccg caggccttca gtcaggcgct
     1981 tcaggacggc atgagcgtcc cgctctatat tcatctcgcc ggtagccagg gtcgccagga
     2041 cgatcagcga atcggcagcg cttttatctg gctggatgat ggacagctac gcatccggaa
     2101 aatacagctg gaagagagtg aagataacgc cagtgtcagc gaacaaactc gacagcagct
     2161 gatggctctg gcgaacgccc cgttcaatga ggcccttacc atccccctga ctgacaacgc
     2221 gcagctggat ctcagcttgc gccaactgct gctgcagctg gtggtcaagc gcgaagcgct
     2281 gggcaccgta ctacgctcac gtagcgaaga catcgggcag tccagtgtta acaccctcag
     2341 cagtaatctg agctataact tgggcgtcta taacaaccag ttgcgtaacg gcgggagcaa
     2401 cacatccagc tatctgtcgc tgaataacgt tactgcgctg cgcgaacatc atgtggtgct
     2461 cgacggctcg ctgtacggga tcggtagcgg tcaacaggac agtgaattat ataaagcgat
     2521 gtatgaacgc gattttgccg gtcaccgatt tgccggtgga atgctcgaca cctggaactt
     2581 gcagtcctta gggccgatga ccgccatttc agcagggaag atttacggcc tttcctgggg
     2641 aaaccaggcc agctccacca tcttcgacag cagccagtca gccacgccag tgatcgcctt
     2701 tttaccggcg gcgggcgaag tacatctcac ccgtgatggg cggctactaa gcgttcagaa
     2761 cttcactatg ggcaatcatg aagtggatac ccggggtcta ccgtacggga tttacgatgt
     2821 ggaagttgag gtgatcgtta acggtcgcgt gatcagcaaa cgcccccagc gggtcaataa
     2881 gctgtttagc cgggggcgcg gcgtcggtgc accactggcg tggcaggtat ggggcggtag
     2941 ctttcatatg gatcgctggt cggaaaacgg gaaaaagacg cgaccagcta aagagagttg
     3001 gctagcaggt gcctcgacct ccggctcatt gagtacgctt agctgggcgg caacgggata
     3061 tggatacgat aatcaggcgg tgggtgaaac ccgtctgacg ctgccgcttg ggggggcgat
     3121 caacgttaac ctgcaaaata tgctggccag tgacagctca tggagcagca tcggcagcat
     3181 cagcaccact ctaccgggag gctttagttc gctgtgggtt aaccaggaaa aaacccgcat
     3241 tggcaatcaa ttgcgacgta gcgatgccga caaccgtgca atcggcggca cactcaacct
     3301 gaactcactg tggtcgaagc tgggcacatt cagcatcagc tacaatgatg accgccgtta
     3361 caacagccat tattacacgg cagattacta tcaaaatgtc tacagcggta cctttggttc
     3421 gcttggcctg cgggccggta ttcagcgcta taacaacggc gacagcaacg ccaatacagg
     3481 gaaatatatc gctctcgatc tctcgctacc actgggcaac tggtttagcg cagggatgac
     3541 tcatcaaaac ggctacacca tggcaaacct gtcagcacgc aaacagtttg atgaaggaac
     3601 cattcgcact gttggtgcca atctgtcacg tgccatctcc ggcgataccg gtgatgacaa
     3661 aaccctcagc ggtggggcgt atgcacagtt cgacgctcgt tacgccagcg gaacgctgaa
     3721 cgtcaatagc gcggcggacg gctacatcaa taccaacttg accgccaatg gcagcgtcgg
     3781 ctggcagggt aaaaacatcg ctgccagcgg gcggactgat ggcaacgctg gggtgatatt
     3841 caacaccggg ctggaggacg acggtcagat cagcgccaaa atcaacgggc ggattttccc
     3901 gcttaacggc aagcgtaact atctcccgct ctctccctat ggaagatatg aggtggagtt
     3961 acagaacagc aaaaactcac tcgacagcta cgatatcgtc agcggccgca aaagccatct
     4021 gactctctat ccaggcaatg tcgctgtcat tgagccagag gtgaagcaga tggttaccgt
     4081 ctccggtcgt atccgtgcgg aagacggcac actgctggct aacgcacgga ttaacaacca
     4141 tatcggccga acccgaaccg atgaaaacgg cgagtttgtc atggacgtgg ataagaaata
     4201 tcccactatc gattttcgct acagtggcaa taaaacctgc gaagtggcac tggaactcaa
     4261 ccaggcgcgc ggtgccgtct gggtcggtga tgtggtctgc agcggcctct catcgtgggc
     4321 ggaggtgacg cagacaggag aagagaatga gagttaacct actaataacg atgataattt
     4381 tcgcgctaat ctggccagta actgagctca gagcggcagt gagcaaaaca acctgggcgg
     4441 atgcaccggc acgcgagttt gtgtttgtcg aaaacaactc agacgacaac tttttcgtca
     4501 ctcctggcgg ggcgctggat ccgcgcctga ccggtgccaa ccgctggacc ggtttaaaat
     4561 acactggttc aggaaccatc tatcagcaaa gcctcggcta cattgataac ggttacaaca
     4621 ccggccttta taccaactgg aagtttgata tgtggctgga aaattcacca gtttcatctc
     4681 ctttaactgg cttgcgctgc atcaactggt acgctgggtg taatatgacc accagtctta
     4741 tcctgccgca aaccaccgac gccagtggat tttatggcgc gacggtgacc agcggcggcg
     4801 cgaagtggat gcacggcatg ttgtcagacg cgttttacca gtatctgcaa caaatgcccg
     4861 tcggcagcag ctttacaatg accatcaatg cctgccagac ctctgtgaac tatgacgcca
     4921 gcagcggcgc acgctgtaag gatcaggcct ccggcaactg gtatgttcgc aacgtcaccc
     4981 atacgaaagc agcaaatctg cggttaataa atacccactc gctggcggag gtatttatca
     5041 acagcgacgg agtaccgact ctgggcgaag ggaacgccga ctgccggaca cagaccatcg
     5101 gcagcctttc aggattaagt tgtaagatgg tcaactatac cctgcaaaca aacggactca
     5161 gcaacacctc aatccatata ttcccggcga tcgccaactc gtcgttagcc tcggccgtcg
     5221 gggcgtacga tatgcagttc agtctgaatg gcagttcatg gaaaccggtg agcaataccg
     5281 cctattacta caccttcaac gagatgaaga gcgcagactc gatctatgtt ttcttctcga
     5341 gcaacttctt taagcagatg gtgaacctcg ggatcagcga tatcaacacc aaagatctat
     5401 tcaactttcg ctttcagaac accacatcac cggagtctgg ctggtatgaa ttttctacct
     5461 ccaacacgct gattatcaaa ccccgtgatt tcagcatcag tattatctcc gatgaatata
     5521 ctcagacacc gtcgcgggag ggatatgttg gcagcggcga gtcggcactc gatttcggct
     5581 atatcgtaac caccagcggt aaaacagctg ccgacgaagt gctgatcaag gtgaccggac
     5641 ccgcgcaggt gattggcggg cgctcctatt gtgtcttcag ctccgatgac ggtaaggcga
     5701 aagtaccgtt cccggcgaca ctttccttta ttacccgcaa cggagctaca aaaacctacg
     5761 atgccgggtg cgatgatagc tggcgggata tgaccgatgc gctgtggttg accacaccgt
     5821 ggactgatat ctctggcgaa gtggggcaga tggataagac cacagtcaaa ttttcgattc
     5881 caatggataa cgccatttcg ctgcgtacgg tagatgataa cggctggttt ggcgaagtca
     5941 gcgcttcagg agagattcat gttcaggcga cgtggcgtaa cattaactaa ggccctgctg
     6001 acagcggtct gtatgctggc ggcacctttg acacaggcga tttcggtcgg caatctgaca
     6061 ttttcgctgc cgtccgagac tgactttgtc agcaaacgtg tagtgaataa caacaaaagc
     6121 gcacggatat accgtattgc catcagtgct attgatagcc cgggcagcag tgaattgcgc
     6181 acccgaccgg tggatggtga actgcttttt gccccccgcc aactggcgtt gcaggctggt
     6241 gagagcgagt attttaaatt ttactatcat ggtccacggg ataaccgcga gcgctactac
     6301 cgggtctcat ttcgcgaagt ccccactcgt aaccagacaa gacgtagccc aaccggcggc
     6361 gaggtcagca cggagccggt ggtggtgatg gataccattc tggtagtacg accacgtcag
     6421 gttcagttta aatggtcctt cgacaaggtg acaggaacgg taagtaacac tggcaacaca
     6481 tggtttaagc tactgattaa gccaggatgt gattcgaccg aagaggaagg cgatgcctgg
     6541 tatctacgtc cgggagacgt ggttcatcag cctgagttac gtcagccggg gaatcattat
     6601 ctggtctata acgacaaatt cattaagatt agcgattcct gtccggctaa gcccccttcg
     6661 gcggactaag acttatccat cggtcgaggg aagaattcca cctcagagct ccaaaaatcg
     6721 tttaaatgat gtggaagcga tcgtcaatgt cacctgtaac gatgggcttt ctcatatgca
     6781 caccatagtg ttcaggagac acggaaaggt atcaggcagc ctgctaatga gtggtttaat
     6841 cacgaattag tacgtaaaat cggtaacggc tggaaatcat tcaatactca cactatcgaa
     6901 agttccccag ccaaccgcgg tacgttctta catacgatgt accgctgttc tctttacgat
     6961 ttttagctgt actggtgaat tatgagcaat ctgaatccat gcatgacgtg tggtgcctgt
     7021 tgtgcatttt tccgcgtctc tttttactgg gccgaagccg acgatgctgg cggtactatt
     7081 cccgccaggc tcactgaaca aatatcccct tttcaccgat gcatgagcgg taccaatcag
     7141 aaaaaccccc gatgtattgc ccttgcagga accccgggca aaaatgcctg ctgcacgata
     7201 tataaaaatc gatcgtccac atgcagagaa tttgccatgt ctggtgagaa cggagtcgtc
     7261 aatgaggctt gcaatcgtgc aagggctaaa tacgggctg
//
LOCUS       DAZKHF020000213         7299 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2201S31241
            SAMN43267800-rid25106413.denovo.215, whole genome shotgun sequence.
ACCESSION   DAZKHF020000213 DAZKHF020000000
VERSION     DAZKHF020000213.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267800
            Sequence Read Archive: SRR30307263
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 7299)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7299)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 7299)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350581.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350581.2
            Genome Coverage       :: 151x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:21:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,652
            CDSs (total)                      :: 5,557
            Genes (coding)                    :: 5,320
            CDSs (with protein)               :: 5,320
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 237
            CDSs (without protein)            :: 237
            Pseudo Genes (ambiguous residues) :: 0 of 237
            Pseudo Genes (frameshifted)       :: 102 of 237
            Pseudo Genes (incomplete)         :: 130 of 237
            Pseudo Genes (internal stop)      :: 52 of 237
            Pseudo Genes (multiple problems)  :: 41 of 237
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7299
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267800-rid25106413.denovo.215"
                     /serotype="O 26:H11"
                     /isolate="2201S31241"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-01"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            82..213
                     /locus_tag="ACHOSC_RS18625"
                     /old_locus_tag="pgaptmp_003725"
     ncRNA           82..213
                     /ncRNA_class="other"
                     /locus_tag="ACHOSC_RS18625"
                     /old_locus_tag="pgaptmp_003725"
                     /product="RtT sRNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00391"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="rtT sRNA, processed from tyrT transcript; Derived
                     by automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF00391"
     gene            complement(397..1074)
                     /gene="narI"
                     /locus_tag="ACHOSC_RS18630"
                     /old_locus_tag="pgaptmp_003726"
     CDS             complement(397..1074)
                     /gene="narI"
                     /locus_tag="ACHOSC_RS18630"
                     /old_locus_tag="pgaptmp_003726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009325 - nitrate reductase complex
                     [Evidence IEA];
                     GO_function: GO:0008940 - nitrate reductase activity
                     [Evidence IEA];
                     GO_process: GO:0019645 - anaerobic electron transport
                     chain [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="respiratory nitrate reductase subunit gamma"
                     /protein_id="HGU4549580.1"
                     /translation="MQFLNMFFFDIYPYIAGAVFLIGSWLRYDYGQYTWRAASSQMLD
                     RKGMNLASNLFHIGILGIFVGHFFGMLTPHWMYEAWLPIEVKQKMAMFAGGASGVLCL
                     IGGVLLLKRRLFSPRVRATTTGADILILSLLVIQCALGLLTIPFSAQHMDGSEMMKLV
                     GWAQSVVTFHGGASQHLDGVAFIFRLHLVLGMTLFLLFPFSRLVHIWSVPVEYLTRKY
                     QLVRARH"
     gene            complement(1074..1784)
                     /gene="narJ"
                     /locus_tag="ACHOSC_RS18635"
                     /old_locus_tag="pgaptmp_003727"
     CDS             complement(1074..1784)
                     /gene="narJ"
                     /locus_tag="ACHOSC_RS18635"
                     /old_locus_tag="pgaptmp_003727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0051131 - chaperone-mediated protein
                     complex assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase molybdenum cofactor assembly
                     chaperone"
                     /protein_id="HGU4549581.1"
                     /translation="MIELVIVSRLLEYPDAALWQHQQEMFEAIAASKNLSKEDAHALG
                     IFLRDLTAMDPLDAQAQYSELFDRGRATSLLLFEHVHGESRDRGQAMVDLLAQYEQHG
                     LQLNSRELPDHLPLYLEYLSQLPQSEAVEGLKDIAPILALLSARLQQRESRYAVMFDL
                     LLKLANTAIDSDKVAEKIADEARDDTPQALDAVWEEEQVKFFADKGCGDSAITAHQRR
                     FAGAVAPQYLNITTGGQH"
     gene            complement(1781..3319)
                     /gene="narH"
                     /locus_tag="ACHOSC_RS18640"
                     /old_locus_tag="pgaptmp_003728"
     CDS             complement(1781..3319)
                     /gene="narH"
                     /locus_tag="ACHOSC_RS18640"
                     /old_locus_tag="pgaptmp_003728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009325 - nitrate reductase complex
                     [Evidence IEA];
                     GO_function: GO:0008940 - nitrate reductase activity
                     [Evidence IEA];
                     GO_process: GO:0042126 - nitrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase subunit beta"
                     /protein_id="HGU4549582.1"
                     /translation="MKIRSQVGMVLNLDKCIGCHTCSVTCKNVWTSREGVEYAWFNNV
                     ETKPGQGFPTDWENQEKYKGGWIRKINGKLQPRMGNRAMLLGKIFANPHLPGIDDYYE
                     PFDFDYQNLHTAPEGSKSQPIARPRSLITGERMAKIEKGPNWEDDLGGEFDKLAKDKN
                     FDNIQKAMYSQFENTFMMYLPRLCEHCLNPACVATCPSGAIYKREEDGIVLIDQDKCR
                     GWRMCITGCPYKKIYFNWKSGKSEKCIFCYPRIEAGQPTVCSETCVGRIRYLGVLLYD
                     ADAIERAASTENEKDLYQRQLEVFLDPNDPKVIEQAIKDGIPLSVIEAAQQSPVYKMA
                     MEWKLALPLHPEYRTLPMVWYVPPLSPIQSAADAGELGSNGILPDVESLRIPVQYLAN
                     LLTAGDTKPVLRALKRMLAMRHYKRAETVDGKVDTRALEEVGLTEAQAQEIYRYLAIA
                     NYEDRFVVPSSHRELAREAFPEKNGCGFTFGDGCHGSDTKFNLFNSRRIDAIDVTSKT
                     EPHP"
     gene            complement(3316..7059)
                     /locus_tag="ACHOSC_RS18645"
                     /old_locus_tag="pgaptmp_003729"
     CDS             complement(3316..7059)
                     /locus_tag="ACHOSC_RS18645"
                     /old_locus_tag="pgaptmp_003729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009325 - nitrate reductase complex
                     [Evidence IEA];
                     GO_function: GO:0008940 - nitrate reductase activity
                     [Evidence IEA];
                     GO_process: GO:0042126 - nitrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase subunit alpha"
                     /protein_id="HGU4549583.1"
                     /translation="MSKFLDRFRYFKQKGETFADGHGQLLNTNRDWEDGYRQRWQHDK
                     IVRSTHGVNCTGSCSWKIYVKNGLVTWETQQTDYPRTRPDLPNHEPRGCPRGASYSWY
                     LYSANRLKYPMMRKRLMKMWREAKALHSDPVEAWASIIEDADKAKSFKQARGRGGFVR
                     SSWQEVNELIAASNVYTIKNYGPDRVAGFSPIPAMSMVSYASGARYLSLIGGTCLSFY
                     DWYCDLPPASPQTWGEQTDVPESADWYNSSYIIAWGSNVPQTRTPDAHFFTEVRYKGT
                     KTVAVTPDYAEIAKLCDLWLAPKQGTDAAMALAMGHVMLREFHLDNPSQYFTDYVRRY
                     TDMPMLVMLEERDGYYAAGRMLRAADLVDALGQENNPEWKTVAFNTNGEMVAPNGSIG
                     FRWGEKGKWNLEQRDGKTGEETELQLSLLGSQDEIAEVGFPYFGGDGTEHFNKVELEN
                     VLLHKLPVKRLQLADGSTALVTTVYDLTLANYGLERGLNDVNCATSYDDVKAYTPAWA
                     EQITGVSRSQIIRIAREFADNADKTHGRSMIIVGAGLNHWYHLDMNYRGLINMLIFCG
                     CVGQSGGGWAHYVGQEKLRPQTGWQPLAFALDWQRPARHMNSTSYFYNHSSQWRYETV
                     TAEELLSPMADKSRYTGHLIDFNVRAERMGWLPSAPQLGTNPLTIAGEAEKAGMNPVD
                     YTVKSLKEGSIRFAAEQPENGKNHPRNLFIWRSNLLGSSGKGHEFMLKYLLGTEHGIQ
                     GKDLGQQGGVKPEEVDWQDNGLEGKLDLVVTLDFRLSSTCLYSDIILPTATWYEKDDM
                     NTSDMHPFIHPLSAAVDPAWEAKSDWEIYKAIAKKFSEVCVGHLGKETDIVTLPIQHD
                     SAAELAQPLDVKDWKKGECDLIPGKTAPHIMVVERDYPATYERFTSIGPLMEKISNGG
                     KGIAWNTQSEMDLLRKLNYTKAEGPAKGQPMLNTAIDAAEMILTLAPETNGQVAVKAW
                     AALSEFTGRDHTHLALNKEDEKIRFRDIQAQPRKIISSPTWSGLEDEHVSYNAGYTNV
                     HELIPWRTLSGRQQLYQDHQWMRDFGESLLVYRPPIDTRSVKEVMGQKSNGNPEKALN
                     FLTPHQKWGIHSTYSDNLLMLTLGRGGPVVWLSEADAKDLGIADNDWIEVFNSNGALT
                     ARAVVSQRVPAGMTMMYHAQERIVNLPGSEITQQRGGIHNSVTRITPKPTHMIGGYAH
                     LAYGFNYYGTVGSNRDEFVVVRKMKNIDWLDGEGNDQVQESVK"
BASE COUNT         1638 a         2062 c         1887 g         1712 t
ORIGIN      
        1 gacttcgaag gttcgaatcc ttcccccacc accatcactt ttcaaaagtc cgtaaactct
       61 caaacgaatc cgcaattaaa tattctgccc aagcggggaa ggatgagaag cttcgaccaa
      121 ggttcgactt gagcgccagc gagagagcgt tgccgcaggc aacgacccga agggcgaagc
      181 gcgcagcgtt gagtaatcct tcccccacca ccatcacttc cttaaataat caaaacctgt
      241 tactccgagc taattttgcc cgatggcacc acgtttaccg ggcctacaat atcctcccct
      301 gtaggtcgga taagcgaagc gcatccgaca actcaccccg caccgtgccc ataaaaaaac
      361 cccgccgaag cagggtctat gtgaactaaa attcgcttag tgacgagcgc gcaccagctg
      421 gtacttacgt gtcagatact ccaccggtac gctccagatg tgtaccagac gcgagaacgg
      481 gaacagcagg aataacgtca tccccagcac caggtgcaga cggaagataa acgccacacc
      541 atcgaggtgt tgagaagcgc caccgtggaa ggtcaccacc gactgcgccc agccaaccag
      601 tttcatcatc tcgctaccgt ccatatgctg agcggagaac ggaatggtca acaggcccag
      661 cgcgcactgg ataacgagca gcgacaggat caggatatcc gctccggtag tggttgcacg
      721 cacgcgtggg ctgaacagac gacgcttcag caacagcacg ccgccaatca gacacagcac
      781 gccgctggca ccaccagcaa acatcgccat tttctgtttc acttcaatcg gcagccaggc
      841 ttcatacatc cagtgcggcg tcagcatacc gaagaagtga ccaacaaaaa tccccagaat
      901 accgatatgg aacaggttcg acgccaggtt catccctttg cgatccagca tctggctgga
      961 cgccgcgcgc caggtgtact gcccgtagtc ataacgcagc cagctaccaa tcaggaagac
     1021 cgccccggct atgtacgggt agatatcaaa gaagaacata ttcaggaatt gcattagtgc
     1081 tgtcctccgg tggtgatatt cagatattgc ggcgcgacgg caccggcaaa gcgacgctga
     1141 tgagcagtga ttgctgaatc accgcagcct ttgtcagcaa agaatttaac ctgctcttct
     1201 tcccagacag catccagcgc ctgcggcgta tcatcgcgcg cttcgtcggc aattttttcc
     1261 gccactttgt cgctgtcgat agcggtattg gccaatttca gcagcagatc aaacatcacg
     1321 gcataacggc tttcacgctg ttgcagacgc gcgctcagca atgccagaat cggcgcgata
     1381 tctttcaaac cttccacggc ttcgctttgc ggcagctgcg acaggtactc cagatacagc
     1441 ggcagatggt ccggtaattc gcggctgttt aactgcaagc cgtgctgctc gtactgcgcc
     1501 agcaggtcca ccatcgcctg gccgcggtca cgggattcac cgtgaacatg ttcaaatagc
     1561 aacagtgacg tggcgcggcc acggtcgaac agttcgctgt actgggcctg ggcatcgagc
     1621 ggatccatcg ccgttaaatc gcgcaggaaa atgcccagcg catgggcatc ttctttcgac
     1681 agatttttcg acgcagcaat cgcctcaaac atctcttgtt gatgctgcca taaggcagca
     1741 tccggatatt caaggagacg cgatacaatc acgagttcga tcatggatgc ggctccgttt
     1801 tgctggtcac atcgatggca tcgatacgac ggctgttgaa cagattgaat ttggtatctg
     1861 aaccgtggca gccatcacca aaggtaaagc cgcagccatt tttctccggg aaggcttccc
     1921 gtgccagttc acgatgacta ctcggcacca caaagcgatc ttcgtagtta gcaatcgcca
     1981 gataacggta tatctcctgt gcctgggctt cggtcagacc gacctcttcc agcgcacggg
     2041 tatcaacttt accgtcaacg gtttcagcac gtttgtaatg acgcatcgcc aacatacgtt
     2101 tcagtgcgcg cagtaccggt ttggtatcac cggcggtcag cagattcgcc agatactgta
     2161 ccggaatacg caagctttcg acgtctggca gaatgccgtt gctacccagc tcacccgcgt
     2221 ctgctgcaga ctgaatcgga gacagaggcg gcacgtacca gaccatcggc agtgtgcgat
     2281 attctggatg cagcggcagc gccagtttcc attccattgc cattttataa accggcgact
     2341 gctgtgcggc ttcaataacg ctcagcggaa taccgtcttt aatcgcctgc tcgatgactt
     2401 tcggatcgtt cggatcgagg aacacctcca gctgacgctg gtaaagatct ttctcgttct
     2461 cggtgctggc tgcacgttca atcgcgtcgg catcgtacag cagcacgcca agataacgga
     2521 tacgaccgac acaggtttct gagcacacgg tcggctgacc cgcttcaata cgcggatagc
     2581 agaagatgca cttctcagac ttaccgctct tccagttgaa gtagattttt ttgtacgggc
     2641 atccggtgat gcacatacgc cagccacggc atttatcctg gtcgatcagg acgatgccat
     2701 cttcttcacg cttgtaaatc gcaccgctcg ggcaggtcgc cacacatgcc gggttcaggc
     2761 agtgttcgca caggcgcggc aaatacatca taaaggtgtt ttcgaactgg ctatacatcg
     2821 ccttctggat gttgtcgaag ttcttgtctt tcgccagttt gtcaaactca ccacccaggt
     2881 catcttccca gttcggccct ttttcgattt tcgccatccg ttccccggta atcagcgaac
     2941 gcggacgggc aatcggctgc gatttgctgc cttccggcgc ggtatgcagg ttctgatagt
     3001 caaaatcgaa cggctcgtaa taatcgtcga tccccggcag atgcgggtta gcgaagattt
     3061 tacccagcaa catggcacgg ttacccatgc gcggctgcag tttgccgttg attttacgga
     3121 tccagccgcc tttgtatttt tcctggtttt cccagtcagt cgggaagccc tgacccggct
     3181 tggtttccac gttgttgaac cacgcgtatt ccacgccttc acggctggtc cagacgtttt
     3241 tacaggtaac tgaacaggtg tggcagccga tgcacttatc gagattcagc accatgccga
     3301 cttgtgaacg aattttcatt ttacgctctc ctgtacctgg tcattgcctt cgccatctaa
     3361 ccagtcaatg ttcttcatct tacgcactac aacaaactca tcgcggttag aacctacggt
     3421 gccgtagtag ttaaagccgt atgccagatg ggcatagccg ccgatcatat gcgtcggttt
     3481 cggcgtgata cgggtgaccg agttatggat accaccacgc tgttgggtaa tttccgaacc
     3541 aggcaggtta acgatacgtt cctgcgcgtg gtacatcatg gtcatccctg ccggaacacg
     3601 ctggctgaca accgcacggg cagtcagagc accgttgctg ttgaagactt caatccagtc
     3661 gttatcggcg atacccagat ctttggcatc ggcttcactc aaccagacca ccggaccacc
     3721 gcgacctaaa gtcagcatca gcaggttgtc gctgtaggtg gagtggatac cccacttctg
     3781 gtgcggcgtc aggaagttga gcgctttttc cgggttgcca ttggatttct ggcccatcac
     3841 ttctttcacc gaacgggtgt cgatcggcgg acgataaacc agcaggcttt caccgaaatc
     3901 acgcatccat tggtgatcct gatacagctg ctgacggcca gagagcgtac gccacgggat
     3961 cagctcgtga acgttggtgt aaccggcgtt gtaagaaacg tgttcatctt caaggccaga
     4021 ccaggtcggg ctggagatga ttttgcgcgg ctgtgcctga atatcgcgga agcgaatctt
     4081 ctcgtcttct ttattcagcg ccagatgcgt atggtcacga ccggtaaact cgctcagggc
     4141 agcccaggct ttcacggcta cctgaccgtt ggtttccggt gccagcgtca ggatcatctc
     4201 tgccgcatca attgcggtgt tcagcatcgg ctggcctttc gccggacctt ccgctttggt
     4261 gtagttgagc ttacgcagca gatccatctc gctctgggtg ttccaggcaa tccctttacc
     4321 gccattactg attttctcca tcagcgggcc gatagaggta aagcgttcgt aagtcgccgg
     4381 ataatcgcgc tctacgacca taatgtgtgg cgcggtttta ccagggatca ggtcgcactc
     4441 gccttttttc cagtctttca catccagcgg ctgcgccagt tcagcggcag agtcgtgctg
     4501 gataggcagc gtgacgatgt cggtttcttt acccagatga ccgacgcaca cttcggagaa
     4561 tttcttcgcg atggctttgt aaatttccca gtcgcttttc gcttcccagg ccggatcgac
     4621 cgccgcagac agcgggtgaa taaacggatg catatccgaa gtattcatgt cgtctttttc
     4681 gtaccaggtc gccgtcggca aaatgatgtc ggaatagaga caggtgctcg acagacggaa
     4741 gtccagcgta accaccagat ccagcttgcc ttccagacca ttgtcctgcc agtccacttc
     4801 ttccggcttc acgccgccct gttgccccag atctttaccc tggataccgt gctccgtccc
     4861 cagcaggtac ttgagcataa actcatgacc tttaccggaa gaaccgagca ggttagaacg
     4921 ccagatgaac aggttgcgcg ggtggttttt accgttttct ggttgttctg ccgcaaaacg
     4981 gatggaaccc tctttcaggg atttcaccgt atagtccacc ggattcatcc cggctttttc
     5041 cgcttcgcca gcgatagtca gcgggttagt gcctaactgc ggtgcagacg gcagccagcc
     5101 catgcgttcc gcacggacgt taaagtcgat caagtgtccg gtatagcggg atttgtccgc
     5161 catcggtgac agcaactctt ccgccgtgac ggtttcataa cgccactggc tggagtggtt
     5221 atagaaataa gaagtgctgt tcatgtgacg cgccggacgc tgccagtcaa gggcaaacgc
     5281 cagcggctgc cagccggttt gcggacgcag tttttcctga cctacatagt gcgcccagcc
     5341 gcccccgctc tgaccgacac agccgcagaa aatcagcatg ttgatcagac cacgatagtt
     5401 catatcgagg tgataccagt ggttcagccc cgcaccgacg ataatcatcg aacgaccgtg
     5461 cgttttatca gcgttatcgg caaattcacg ggcgatgcga ataatctggc tgcgagaaac
     5521 gccggtaatc tgctcggccc aggccggggt ataagctttc acatcgtcat agctggttgc
     5581 acagttaacg tcgttcaggc cacgttccag accgtagttt gccagcgtca gatcataaac
     5641 ggtggtcacc agggcggtgc tgccatcagc cagttgcagg cgtttcaccg gcagtttgtg
     5701 cagcagcacg ttttccagtt ccactttgtt gaaatgttcc gtgccgtcgc caccaaagta
     5761 cgggaagcct acctcagcga tctcatcctg gctacccagc aggctcagtt gcagctcggt
     5821 ttcttcgcca gttttgccgt cgcgctgctc aagattccat ttgcccttct cgccccagcg
     5881 gaagccaata gaaccgttcg gcgcaaccat ttcgccattg gtattaaagg cgacagtttt
     5941 ccattccgga ttgttttcct ggcccagcgc atcaaccaga tcagcagcgc gcagcatacg
     6001 acctgcagcg tagtaaccgt cgcgttcttc cagcatcacc agcatcggca tgtcggtgta
     6061 gcgacgcaca tagtcggtga aatactggct tgggttgtcg aggtggaatt cacgcagcat
     6121 tacgtggccc atcgccagcg ccattgccgc atcggtgccc tgtttcggtg ccagccacag
     6181 atcgcacagt ttggcgattt cagcgtagtc tggtgtgacg gcaacagttt tggtcccttt
     6241 gtaacgcact tcagtaaaga agtgagcatc cggggtacgc gtctgcggta cgtttgaccc
     6301 ccaggcgatg atgtagctgg agttgtacca gtcagcagat tccggtacgt cagtttgctc
     6361 gccccaggtt tgcggagacg caggaggcaa gtcgcagtac cagtcgtaga agcttaagca
     6421 agtaccgcca atcagcgaga gatagcgtgc acccgatgcg taagaaacca tcgacattgc
     6481 cggaattggc gagaaaccag caacacggtc cgggccgtag tttttgatgg tgtaaacgtt
     6541 agatgcggcg atcagttcgt tcacctcctg ccaggaagaa cgaacaaatc caccgcgtcc
     6601 acgcgcctgc ttaaagcttt tcgctttatc ggcgtcttca atgatagaag cccatgcctc
     6661 aaccggatcg ctatgcagcg ccttcgcttc acgccacatt ttcatcaggc gtttgcgcat
     6721 catcgggtat ttcaggcggt tggcactgta aagataccag gagtagctgg caccgcgcgg
     6781 gcagccgcga ggttcatggt ttggcagatc cggacgggta cgcggatagt cagtctgctg
     6841 ggtttcccag gtgaccagac cgtttttgac gtagattttc cagctgcagg agccggtgca
     6901 gtttaccccg tgggtagagc ggacgatttt gtcatgctgc caacgctggc gatatccatc
     6961 ctcccagtca cggttggtat tgagaagctg gccatgccca tcggcaaagg tttcaccctt
     7021 ctgtttgaag tagcgaaacc ggtccaggaa tttactcatc ggttttctcc tgtgggagcc
     7081 tgtcggccct ctgataaatc gacattgcta atgtttaagg gcaaggtaac gctctgaaac
     7141 agcgtgggaa ttgataacga tcaagtgtga tggggatgaa aaataaagta aattcctaag
     7201 ataccccttt aggataatta acagttaaag cataacatgc tgtaaattaa gacgttttat
     7261 acctgtagtt atttaaagca tgaagatttt tatgcagat
//
LOCUS       DAZKHF020000214         7286 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2201S31241
            SAMN43267800-rid25106413.denovo.216, whole genome shotgun sequence.
ACCESSION   DAZKHF020000214 DAZKHF020000000
VERSION     DAZKHF020000214.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267800
            Sequence Read Archive: SRR30307263
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 7286)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7286)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 7286)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350581.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350581.2
            Genome Coverage       :: 151x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:21:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,652
            CDSs (total)                      :: 5,557
            Genes (coding)                    :: 5,320
            CDSs (with protein)               :: 5,320
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 237
            CDSs (without protein)            :: 237
            Pseudo Genes (ambiguous residues) :: 0 of 237
            Pseudo Genes (frameshifted)       :: 102 of 237
            Pseudo Genes (incomplete)         :: 130 of 237
            Pseudo Genes (internal stop)      :: 52 of 237
            Pseudo Genes (multiple problems)  :: 41 of 237
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7286
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267800-rid25106413.denovo.216"
                     /serotype="O 26:H11"
                     /isolate="2201S31241"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-01"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            complement(<1..978)
                     /gene="pyrD"
                     /locus_tag="ACHOSC_RS18650"
                     /old_locus_tag="pgaptmp_003730"
     CDS             complement(<1..978)
                     /gene="pyrD"
                     /locus_tag="ACHOSC_RS18650"
                     /old_locus_tag="pgaptmp_003730"
                     /EC_number="1.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004152 - dihydroorotate dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006222 - UMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="quinone-dependent dihydroorotate dehydrogenase"
                     /protein_id="HGU4549584.1"
                     /translation="MYYPFVRKALFQLDPERAHEFTFQQLRRITGTPFEALVRQKVPA
                     KPVNCMGLTFKNPLGLAAGLDKDGECIDALGAMGFGSIEIGTVTPRPQPGNDKPRLFR
                     LVDAEGLINRMGFNNLGVDNLVENVKKAHYDGVLGINIGKNKDTPVEQGKDDYLICME
                     KIYAYAGYIAINISSPNTPGLRTLQYGEALDDLLTAIKNKQNDLQAMHHKYVPIAVKI
                     APDLSEEELIQVADSLVRHNIDGVIATNTTLDRSLVQGMKNCDQTGGLSGRPLQLKST
                     EIIRRLSLELNGRLPIIGVGGIDSVIAAREKIAAGASLVQIYSGFIFKGP"
     gene            complement(1089..1826)
                     /locus_tag="ACHOSC_RS18655"
                     /old_locus_tag="pgaptmp_003731"
     CDS             complement(1089..1826)
                     /locus_tag="ACHOSC_RS18655"
                     /old_locus_tag="pgaptmp_003731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial chaperone"
                     /protein_id="HGU4549585.1"
                     /translation="MPARHLYFIMTNTWNRLALLIFAVLSLLVAGELRAGVVVGGTRF
                     IFPADRESISILLTNTSQESWLINSKINRPTRWAGGEASTVPAPLLAAPPLILLKPGT
                     TGTLRLLRTESDILPVDRETLFELSIASVPSGKVENQSVKVAMRSVFKLFWRPEGLPG
                     DPLEAYQQLRWTRNSQGVQLTNPTPYYINLIQLSVNGKALSNAGVVPPKSQRQTSWCQ
                     AIAPCHVAWRAINDYGGLSAKKEQNLP"
     gene            complement(1792..2307)
                     /locus_tag="ACHOSC_RS18660"
                     /old_locus_tag="pgaptmp_003732"
     CDS             complement(1792..2307)
                     /locus_tag="ACHOSC_RS18660"
                     /old_locus_tag="pgaptmp_003732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415463.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HGU4549586.1"
                     /translation="MLKRIIWILCLLGLTGSSELFAHDGTVNISGSFRRNTCVLAQDS
                     KQINVQLGDVSLTRFSHGNYGPEKSFIINLQDCGTDVSTVDVTFSGTPDGVQSEMLSI
                     ESGMDAASGLAIAILDDAKILIPLNQASKDYSLHSGKVPLTFYAQLRPVNSDVQSGKV
                     NASATFVLHYD"
     gene            complement(2315..2857)
                     /locus_tag="ACHOSC_RS18665"
                     /old_locus_tag="pgaptmp_003733"
     CDS             complement(2315..2857)
                     /locus_tag="ACHOSC_RS18665"
                     /old_locus_tag="pgaptmp_003733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HGU4549587.1"
                     /translation="MKKKTIYQYVILFFSLLNIHVGMAGPEQVSMHIYGNVVDQGCDV
                     ATKSALQNIHIGDFNISDFQAANTVSTAADLNIDITGCAAGITGADVLFSGEADTLAP
                     TLLKLTDTGGSGGMATGIAVQILDAQSQQEIPLNQVQPLTPLKAGDNTLKYQLRYKST
                     KAGATGGNATAVLYFDLVYQ"
     gene            complement(2869..3939)
                     /locus_tag="ACHOSC_RS18670"
                     /old_locus_tag="pgaptmp_003734"
     CDS             complement(2869..3939)
                     /locus_tag="ACHOSC_RS18670"
                     /old_locus_tag="pgaptmp_003734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HGU4549588.1"
                     /translation="MQIIFGEKCVLLLRLFFAAVLMLWCAQTAAYSGQCHTTQGNPYI
                     GVNFGVKTLEEEENTAGVVKDKFYQWNESNDYYVSCDCDKDNVRSGRWAFAADSPLVY
                     LGDNWYKINDYLAAKVLLQVKGSSPTAVPFENVGTGADTRWHICDPGGQRLGGQGASG
                     NSGSFSLKILQPFVGSVVIPPMALARLFECYNIPAGDSCTTTGTPVLVYYLSGTINSL
                     GSCSVNAGETIEVDLGDVFAANFRVVGHKPLGARTAELAIPVRCNTGNEGLVNVNLSL
                     TATSDPSYPQAIKTSRPGVGVVVTDSQNNIISPAGGTLPLSIPDDADSIARMNVYPVS
                     TTGVPPETGRFEATATVRINFD"
     gene            complement(3930..6530)
                     /locus_tag="ACHOSC_RS18675"
                     /old_locus_tag="pgaptmp_003735"
     CDS             complement(3930..6530)
                     /locus_tag="ACHOSC_RS18675"
                     /old_locus_tag="pgaptmp_003735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706863.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0015473 - fimbrial usher porin activity
                     [Evidence IEA];
                     GO_process: GO:0009297 - pilus assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial biogenesis usher protein"
                     /protein_id="HGU4549589.1"
                     /translation="MYRTHRQHSLLSSGGVPSFIGGLVVFVSAAFNAQAETWFDPAFF
                     KDDPSMVADLSRFEKGQKITPGVYRVDIVLNQTIVDTRNVNFVEITPEKGIAACLTTE
                     SLDAMGVNTDAFPAFKQLDKQACVPLAEIIPDASVTFNVNKLRLEISVPQIAIKSNAR
                     GYVPPERWDEGINALLLGYSFSGANSIHSSADSDSGDSYFLNLNSGVNLGPWRLRNNS
                     TWSRSSGQTAEWKNLSSYLQRAVIPLKGELTVGDDYTAGDFFDSVSFRGVQLASDDNM
                     LPDSLKGFAPVVRGIAKSNAQITIKQNGYTIYQTYVSPGAFEISDLYSTSSSGDLLVE
                     IKEADGSVNSYSVPFSSVPLLQRQGRIKYAVTLAKYRTNSNEQQESKFAQATLQWGGP
                     WGTTWYGGGQYAEYYRAAMFGLGFNLGDFGAISFDATQAKSTLADQSEHKGQSYRFLY
                     AKTLNQLGTNFQLMGYRYSTSGFYTLSDTMYKHMDGYEFNDGDDEDTPMWSRYYNLFY
                     TKRGKLQVNISQQLGEYGSFYLSGSQQTYWHTDQQDRLLQFGYNTQIKDLSLGVSWNY
                     SKSRGQPDADQVFALNFSLPLNLLLPRSNDSYTRKKNYAWMTSNTSIDNEGHITQNLG
                     LTETLLDDGNLSYSVQQGYNSEGKTANGSASMDYKGAFADARVGYNYSDNGSQQQLNY
                     ALSGSLVAHSQGITLGQSLGETNVLIAAPGAENTRVANSTGLKTDWRGYTVVPYATSY
                     RENRIALDAASLKRNVDLENAVVNVVPTKGALVLAEFNAHAGARVLMKTSKQGIPLRF
                     GAIATLDGIQTNSGIIDDDGSLYMSGLPAQGAITVRWGEAPDQICHISYQLTEQQINS
                     AITRMDAICR"
     gene            complement(6555..7256)
                     /locus_tag="ACHOSC_RS18680"
                     /old_locus_tag="pgaptmp_003736"
     CDS             complement(6555..7256)
                     /locus_tag="ACHOSC_RS18680"
                     /old_locus_tag="pgaptmp_003736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0061077 - chaperone-mediated protein
                     folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone"
                     /protein_id="HGU4549590.1"
                     /translation="MKTCITKGIVTVSLTAILLSCSSAWAAGKGGIGLAATRLVYSEG
                     EEQISLGVRNTSPDVPYLIQSWVMTPDNKKSADFIITPPLFVLNPANENLLRIMYIGA
                     PLAKDRETLFFTSVRAVPSTTKREEGNTLKIATQSVIKLFWRPKGLAYPLGEAPAKLR
                     CTSSADMVTVSNPTPYFITLTDLKIGGKVVKNQMISPFDKYQFSLPKGAKNSSVTYRT
                     INDYGAETPQLNCKS"
BASE COUNT         1847 a         1870 c         1627 g         1942 t
ORIGIN      
        1 cggaccttta aaaataaaac cagaataaat ttgcaccagt gaggcacccg cagcaatctt
       61 ttcacgcgca gcgataaccg agtcgatgcc gccaacaccg atgatcggta agcgaccgtt
      121 taattccagt gacaagcggc gaataatttc ggtgcttttt aactgaagcg gacgaccact
      181 taagccaccg gtttgatcgc aatttttcat tccctgaaca agagaacgat cgagtgtggt
      241 attggttgca ataacgccat caatattatg gcgaactaaa ctatcggcaa cctggatcaa
      301 ttcttcttca gaaagatccg gcgcgatctt cactgcgatc ggcacatatt tatggtgcat
      361 cgcttgcaaa tcattttgct tatttttaat cgcggttaag agatcatcca gcgcttcacc
      421 atattgcagc gtgcgtaatc ctggggtatt cggcgatgaa atattgatgg cgatatatcc
      481 cgcataggca tagatttttt ccatacaaat cagatagtca tctttgccct gctccactgg
      541 cgtatcttta tttttgccga tgttaatacc caggacgccg tcataatggg ccttttttac
      601 gttctctacg aggttatcaa cgccaagatt attaaagccc atacggttga tcaaaccttc
      661 ggcatctacc agacgaaaga gacgcggctt gtcattacct ggctgtggac gtggcgtgac
      721 ggtaccgatc tcgatcgatc caaatcccat cgcgcctaac gcgtcaatgc actccccgtc
      781 tttatcaaga ccggctgcca gaccaagcgg atttttaaac gtcaggccca tgcagttaac
      841 aggtttcgca ggcactttct gccgcaccag tgcttcaaac ggcgttcctg taatacggcg
      901 taattgctga aaagtaaact catgagcgcg ctctggatcg agctggaaaa gggctttacg
      961 aacgaagggg tagtacatga actctcctgg attcccggtg tgcaaaccgg gggcgtatta
     1021 tgtgcgagtt cggaccaaaa gggaattgac ctgcggcaaa aaaaacgcaa acgttttccg
     1081 aacctgtttc agggcagatt ttgctccttc tttgcactca acccgccgta atcatttatc
     1141 gcccgccagg cgacatgaca cggtgcgatt gcctgacacc agcttgtctg acgctggctt
     1201 ttaggcggca caactcccgc attacttaac gctttaccat ttacgctcaa ctgaatcagg
     1261 ttaatgtagt aaggcgttgg gttagtgagt tgtacaccct gcgaattccg tgtccagcgt
     1321 aattgttgat aagcttccag cgggtcgccc ggcaaacctt cgggtcgcca gaacagttta
     1381 aataccgagc gcatcgccac ttttacgctc tgattttcaa ctttgccgga tggcacgctg
     1441 gcaatgctta actcaaatag cgtttcgcga tccacaggca agatgtcgct ttccgttctc
     1501 agcaagcgca acgtgcctgt agtaccgggc ttcaggagaa taagtggcgg agcggccagt
     1561 aatggtgctg gcactgtcga cgcttccccc ccggcccaac gcgttgggcg gttgatttta
     1621 ctgttaataa gccaggattc ctgactggta ttagtcagta aaatagatat cgattctctg
     1681 tctgccggaa agataaatcg cgttccgccg accaccactc ctgcccgcaa ttcacccgcc
     1741 accagcagcg ataaaacggc gaaaatcaag agcgccaatc tgttccacgt attagtcata
     1801 atgaagtaca aatgtcgcgc tggcattcac tttacccgac tgcacatcgc tattgacagg
     1861 tcgcaattgc gcataaaaag ttaatggcac cttaccgcta tgtaggctgt agtctttact
     1921 cgcctggtta agcggaatca atatcttcgc atcatccaga atcgcaattg ccagcccgct
     1981 ggctgcatcc ataccacttt caatggacag catttcgctc tgcactccgt cgggggttcc
     2041 tgaaaaggtg acgtcaaccg tgctgacatc tgttccgcaa tcctgcaaat taatgatgaa
     2101 agatttctcc gggccataat tcccgtgaga aaaacgtgtc agcgagacat cgcctaactg
     2161 cacattgatt tgcttgctat cctgtgccag cacgcatgta ttacgacgaa acgatccgct
     2221 aatgttgacc gtgccatcat gagcaaataa ctcactactc cccgttaatc ccagtaagca
     2281 cagaatccag attatccgtt tcaacatcac tccttcactg gtaaaccaga tcaaaataga
     2341 gaaccgccgt cgcattaccg cccgttgctc ccgcctttgt ggacttataa cgaagttgat
     2401 atttgagtgt gttatccccg gcttttaagg gcgtaagagg ctggacctga ttgagcggga
     2461 tttcttgctg actttgcgca tcaagaattt gcacggcaat ccccgttgcc ataccaccgc
     2521 ttccgcctgt gtcagttagt ttaagcagtg tcggcgcaag ggtgtcagcc tcgccgctaa
     2581 aaaggacgtc cgcgccagta ataccagcgg cacaaccggt gatatcgata ttcaaatcag
     2641 ctgcagtgct tacggtattc gcggcctgaa aatcactgat attaaaatcg ccaatatgaa
     2701 tattttgtaa tgcacttttg gtggcgacat cacagccctg atcgaccaca ttcccataaa
     2761 tatgcatact gacttgttca ggcccagcca tcccgacatg gatgttaaga aggctaaaga
     2821 acaaaataac gtactgatat atcgtttttt tcttcatacg ttcacgcgtt aatcaaaatt
     2881 tattctcacc gttgccgtgg cttcaaatcg cccggtttct ggtggtacgc ctgtcgtgct
     2941 gactggatag acattcattc gcgcgatact gtctgcatca tcaggaatag agagcggtag
     3001 tgtcccacca gcaggggaaa taatgttgtt ctggctatcg gtcaccacca cgcccacgcc
     3061 aggacgtgac gtcttaatcg cctggggata gctggggtct gaggttgccg tcagactcag
     3121 gttgacgtta actaaccctt cgtttcccgt gttacacctg actggaattg cgagttctgc
     3181 cgttctggcc ccaaggggtt tatgccctac aacacgaaag ttagccgcaa atacgtcgcc
     3241 cagatcgacc tcgatagtct ctccggcatt gacggaacat gagccaagtg aattgatcgt
     3301 accagacagg taatacacta aaaccggtgt acctgtagtc gtgcaggaat cacctgcggg
     3361 tatgttgtag cattcaaata atcgcgccag cgccatagga ggaataacga ccgaaccaac
     3421 gaacggctgc aatattttca gggaaaagct accgctatta ccgctagccc cctggccacc
     3481 taaacgttga ccgccgggat cgcaaatatg ccatcgtgta tctgcccctg tgcccacgtt
     3541 ttcgaaagga accgcagtag gagaactgcc tttaacctgc aataaaactt tggcggcaag
     3601 atagtcatta attttgtacc agttgtcgcc taaatagact aacggtgaat ccgcggcgaa
     3661 tgcccatcgg ccacttctga cattgtcttt atcgcaatca caggaaacat aataatcatt
     3721 cgattcgttc cactgataaa atttgtcttt aactactcca gccgtatttt cttcttcctc
     3781 cagggtttta acgccaaaat tgacgccaat atacggattc ccctgagtgg tatgacactg
     3841 cccgctataa gcagcagttt gagcgcacca tagcattaag acggcggcaa aaaatagtcg
     3901 tagtaataac acgcattttt ctccaaagat tatctgcata tggcatccat tcgcgtaatc
     3961 gcagagttaa tttgttgttc ggtaagctgg taactgatat gacaaatttg atcgggagct
     4021 tcgccccagc gtacagttat cgctccctga gcgggcaaac cggacatata gagcgagcca
     4081 tcatcatcaa taatgccgct attagtctgt atgccgtcca gcgtcgctat cgcgccaaaa
     4141 cgcagcggta taccctgctt tgatgttttc attaataccc ttgctcccgc atgggcattg
     4201 aactccgcca gaaccaacgc ccctttggtg ggaaccacgt tgactactgc attttcaaga
     4261 tccacgttac gttttaacga tgccgcatca agtgcgattc gattttcccg ataagaagtg
     4321 gcatagggga caacggtata tccgcgccag tcagttttca gcccggtgct gttcgccaca
     4381 cgagtattct ctgcgcctgg tgctgcaatc agcacgttag tttcccccag cgattgcccc
     4441 agggtaatgc cctgtgaatg ggcaactaaa ctgcctgaaa gagcgtagtt cagttgttgt
     4501 tgactgccgt tatcgctgta gttgtagccc actcgggcat ctgcaaacgc ccctttgtag
     4561 tccatgctgg cgctaccatt agccgttttc ccctcgctgt tatatccctg ttgcacgctg
     4621 tagctcaggt taccgtcatc gagtagtgtc tccgttaaac ccaggttttg tgtaatgtgc
     4681 ccttcgttat cgatactggt gttagaggtc atccaggcgt aatttttttt cctggtatag
     4741 ctatcattac ttctggggag caacagattg agcggcaggg aaaaatttag tgcaaacacc
     4801 tgatcagcat caggttgacc acgggactta ctgtagttcc aggaaacccc cagcgagaga
     4861 tctttaattt gcgtgttgta gccaaactgt aatagccgat cctgttgatc ggtatgccag
     4921 taagtttgct ggctaccact taaataaaac gaaccgtact cgcctaattg ctgggagata
     4981 ttgacctgca gtttgccacg tttggtgtaa aacaaattgt aataacgcga ccacatcggc
     5041 gtatcttcat catcaccgtc attaaattcg tagccatcca tatgtttata catggtgtcg
     5101 gaaagggtgt agaaacccga cgtcgaatag cgatagccca tcaattgaaa attagtgccc
     5161 aattggttga gcgttttggc atacagaaaa cgatatgact gacctttatg ttcgctttgg
     5221 tctgccagcg tactcttcgc ctgggtcgca tcgaacgaaa ttgctccgaa atcgccaagg
     5281 ttaaatccca gaccaaacat ggcggcacgg taatattcag catattgtcc accaccatac
     5341 catgtcgttc cccacggtcc gccccactgc aacgtggcct gggcaaattt gctctcctgc
     5401 tgttcattac tattggttct gtatttcgcc agtgtcaccg cgtatttgat tcgcccctga
     5461 cgctggagta atggcacgct ggaaaacggt acgctgtagc tattgacgct gccgtccgct
     5521 tctttgattt caactaacaa atcaccgctc gacgacgtgg aatagagatc actaatttca
     5581 aaagcaccag gcgatacata agtttggtaa atggtgtaac cattttgctt aatcgttatc
     5641 tgggcattgc ttttggcgat accacgcacc acaggcgcaa accctttcag gctgtctggc
     5701 agcatgttgt catctgacgc cagctgcaca ccacgaaagc tgacactatc gaaaaaatcg
     5761 cctgcagtat aatcatcacc tacggtcagt tcgcctttta gtggaataac cgcccgctgc
     5821 aaatagctgc tgagattctt ccattctgcg gtttggccac tactgcgact ccatgttgaa
     5881 ttgttgcgca atctccatgg gcctaaatta acgccactgt ttaaattcag aaaatagctg
     5941 tcgccagaat cactgtctgc gctgctatga atactgttag ccccgctaaa cgaatatccc
     6001 agtaatagcg cgttgatccc ttcatcccaa cgttcagggg ggacataacc acgggcatta
     6061 cttttgatgg cgatttgcgg tactgaaatt tccagacgga gtttattcac attaaacgtt
     6121 acgctggcat ccggaataat ctccgccaat ggcacacacg cttgtttgtc cagttgttta
     6181 aaagccggaa acgcatcagt attaacaccc attgcatcca ggctttcagt cgtcaaacag
     6241 gcggcaatcc ccttctctgg cgttatctca acaaaattga cgttgcgcgt atctacaatt
     6301 gtctgattca gaacaatatc gacacgataa acccctggcg ttattttttg tcctttttcg
     6361 aaacgagaca aatcggccac cattgaggga tcatctttga aaaaagcagg atcgaaccag
     6421 gtttcagctt gtgcattgaa cgctgccgac acaaacacca ccagcccacc aataaacgat
     6481 ggcactccac cagagcttaa caggctgtgt tgtcgatgag ttctatacat ctcgctctct
     6541 taactgaaga cggcttacga tttacagttg agttgcggcg tttccgcccc gtagtcattg
     6601 atggttcgat acgttacgct gctatttttg gccccctttg gcagagaaaa ttggtattta
     6661 tcaaaggggg aaatcatttg atttttaact acttttccac ctattttcag gtctgtcagg
     6721 gtaatgaaat aaggtgttgg gttactgacc gtaaccatgt cagctgacga agtgcaacgc
     6781 agtttcgccg gagcctcgcc taagggatac gctaaacctt ttggtcgcca gaaaagtttg
     6841 atgacgcttt gtgtggcaat cttcagggta tttccctctt cccgcttcgt tgttgaaggg
     6901 actgcccgta cgctagtgaa gaaaagggtt tctctgtctt tcgccaacgg cgcaccaatg
     6961 tacataatgc gtaacagatt ctcattagcc ggattcagca caaatagcgg tggtgtaata
     7021 ataaaatctg ctgatttttt attatctggg gtcatcaccc atgactgaat aagataagga
     7081 acatccggac tggtgttacg tacacccagt gaaatttgct cttcaccttc tgaataaaca
     7141 agacgtgttg ctgcaagtcc aatcccgcct ttacctgccg cccatgcgga tgagcagctg
     7201 agtaatattg ccgttaaact tacggtcaca attccctttg ttatgcaagt tttcataaat
     7261 agtcactctt aaaaaatatg aatatc
//
LOCUS       DAZKHF020000215         7235 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2201S31241
            SAMN43267800-rid25106413.denovo.217, whole genome shotgun sequence.
ACCESSION   DAZKHF020000215 DAZKHF020000000
VERSION     DAZKHF020000215.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267800
            Sequence Read Archive: SRR30307263
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 7235)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7235)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 7235)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350581.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350581.2
            Genome Coverage       :: 151x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:21:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,652
            CDSs (total)                      :: 5,557
            Genes (coding)                    :: 5,320
            CDSs (with protein)               :: 5,320
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 237
            CDSs (without protein)            :: 237
            Pseudo Genes (ambiguous residues) :: 0 of 237
            Pseudo Genes (frameshifted)       :: 102 of 237
            Pseudo Genes (incomplete)         :: 130 of 237
            Pseudo Genes (internal stop)      :: 52 of 237
            Pseudo Genes (multiple problems)  :: 41 of 237
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7235
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267800-rid25106413.denovo.217"
                     /serotype="O 26:H11"
                     /isolate="2201S31241"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-01"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            complement(253..846)
                     /gene="tehB"
                     /locus_tag="ACHOSC_RS18685"
                     /old_locus_tag="pgaptmp_003737"
     CDS             complement(253..846)
                     /gene="tehB"
                     /locus_tag="ACHOSC_RS18685"
                     /old_locus_tag="pgaptmp_003737"
                     /EC_number="2.1.1.265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0046690 - response to tellurium ion
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tellurite resistance methyltransferase TehB"
                     /protein_id="HGU4549591.1"
                     /translation="MIIRDENYFTDKYELTRTHSEVLEAVKVVKPGKTLDLGCGNGRN
                     SLYLAANGYDVDAWDKNAMSIANVERIKSIENLDNLHTRVVDLNNLTFDRQYDFILST
                     VVLMFLEAKTIPGLIANMQRCTKPGGYNLIVAAMDTADYPCTVGFPFAFKEGELRRYY
                     EGWERVKYNEDVGELHRTDANGNRIKLRFATMLARKK"
     gene            complement(843..1835)
                     /gene="tehA"
                     /locus_tag="ACHOSC_RS18690"
                     /old_locus_tag="pgaptmp_003738"
     CDS             complement(843..1835)
                     /gene="tehA"
                     /locus_tag="ACHOSC_RS18690"
                     /old_locus_tag="pgaptmp_003738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dicarboxylate transporter/tellurite-resistance
                     protein TehA"
                     /protein_id="HGU4549592.1"
                     /translation="MQSDKVLNLPAGYFGIVLGTIGMGFAWRYASQVWQVSHWLGDGL
                     VILAMIIWGLLTSAFITRLIRFPHSVLAEVRHPVLSSFVSLFPATTMLVAIGFVPWFR
                     PLAVCLFSFGVVVQLAYAAWQTAGLWRGSHPEEATTPGLYLPTVANNFISAMACGALG
                     YTDAGLVFLGAGVFSWLSLEPVILQRLRSSGELPTALRTSLGIQLAPALVACSAWLSV
                     NGGEGDTLAKMLFGYGLLQLLFMLRLMPWYLSQPFNASFWSFSFGVSALATTGLHLGS
                     GSENGFFHTLAVPLFIFTNFIIAILLIRTFALLMQGKLLVRTERAVLMKAEDKE"
     gene            1959..2939
                     /gene="ydcK"
                     /locus_tag="ACHOSC_RS18695"
                     /old_locus_tag="pgaptmp_003739"
     CDS             1959..2939
                     /gene="ydcK"
                     /locus_tag="ACHOSC_RS18695"
                     /old_locus_tag="pgaptmp_003739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcK family protein"
                     /protein_id="HGU4549593.1"
                     /translation="MRKYRLSEEQRAFSYQEDGTKKNVLLRQIIAISDFNDVIAGTAG
                     GWIDRETVLAQEGNCWIYDQNAIAFGGTVISGNTRITGTSVLWGEVYATDNVWIDNSE
                     ISQGAYISDGVTIHDSLVCGQCRIFGHALINQHSMIVAAQGLTPDHQLLLQIYDRARV
                     SASRIVHQAQIYGDAVIRYAFIEHRAEVFDFASIEGNEENNVWLCDCAKVYGHAQVKA
                     GIEEDAIPTIHYSSQVAEYAIVEGNCVLKHHVLIGGNAVVRGGPILLDEHVVIQGESR
                     ITGAVIIENHVELTDHAVVEAFDGDTVHVRGPKVINGEERITRTPLAGLL"
     gene            complement(2934..3470)
                     /gene="rimL"
                     /locus_tag="ACHOSC_RS18700"
                     /old_locus_tag="pgaptmp_003740"
     CDS             complement(2934..3470)
                     /gene="rimL"
                     /locus_tag="ACHOSC_RS18700"
                     /old_locus_tag="pgaptmp_003740"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707645.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L7/L12-serine
                     acetyltransferase"
                     /protein_id="HGU4549594.1"
                     /translation="MTETIKVSESLELHAVAENHVTPLYQLICKNKTWLQQSLNWPQF
                     VQSEEDTRKTVQGNVMLHQRGYAKMFMIFKEDELIGVISFNRIEPLNKTAEIGYWLDE
                     SHQGQGIISQALQALIHHYAQSGELRRFVIKCRVDNPQSNQVALRNGFILEGCLKQAE
                     FLNDAYDDVNLYARIIDS"
     gene            complement(3533..3757)
                     /locus_tag="ACHOSC_RS18705"
                     /old_locus_tag="pgaptmp_003741"
     CDS             complement(3533..3757)
                     /locus_tag="ACHOSC_RS18705"
                     /old_locus_tag="pgaptmp_003741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415943.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcH family protein"
                     /protein_id="HGU4549595.1"
                     /translation="MFPEYRDLISRLKNENPRFMSLFDKHNKLDHEIARKEGSDGRGY
                     NAEVVRMKKQKLQLKDEMLKILQQESVKEV"
     gene            complement(3897..5552)
                     /gene="opgD"
                     /locus_tag="ACHOSC_RS18710"
                     /old_locus_tag="pgaptmp_003742"
                     /gene_synonym="mdoD"
     CDS             complement(3897..5552)
                     /gene="opgD"
                     /locus_tag="ACHOSC_RS18710"
                     /old_locus_tag="pgaptmp_003742"
                     /gene_synonym="mdoD"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucan biosynthesis protein OpgD"
                     /protein_id="HGU4549596.1"
                     /translation="MDRRRFIKGSMAMAAVCGTSGIASLFSQAAFAADSDIADGQTQR
                     FDFSILQSMAHDLAQTAWRGAPRPLPDTLATMTPQAYNSIQYDAEKSLWHNVENRQLD
                     AQFFHMGMGFRRRVRMFSVDPATHLAREIHFRPELFKYNDAGVDTKQLEGQSDLGFAG
                     FRVFKAPELARRDVVSFLGASYFRAVDDTYQYGLSARGLAIDTYTDSKEEFPDFTAFW
                     FDTVKPGATTFTVYALLDSASITGAYKFTIHCEKSQVIMDVENHLYARKDIKQLGIAP
                     MTSMFSCGTNERRMCDTIHPQIHDSDRLSMWRGNGEWICRPLNNPQKLQFNAYTDNNP
                     KGFGLLQLDRDFSHYQDIMGWYNKRPSLWVEPRNKWGKGTIGLMEIPTTGETLDNIVC
                     FWQPEKAVKAGDEFAFQYRLYWSAQPPVHCPLARVMATRTGMGGFPEGWAPGEHYPEK
                     WARRFAVDFVGGDLKAAAPKGIEPVITLSSGEAKQIEILYIEPIDGYRIQFDWYPTSD
                     STDPVDMRMYLRCQGDAISETWLYQYFPPAPDKRQYVDDRVMS"
     gene            complement(5777..7120)
                     /locus_tag="ACHOSC_RS18715"
                     /old_locus_tag="pgaptmp_003743"
     CDS             complement(5777..7120)
                     /locus_tag="ACHOSC_RS18715"
                     /old_locus_tag="pgaptmp_003743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HGU4549597.1"
                     /translation="MANSITADEIREQFSQAMSAMYQQEVPQYGTLLELVADVNLAVL
                     ENNPQLHEKMVNADELARLNVERHGAIRVGTAQELATLRRMFAIMGMYPVSYYDLSQA
                     GVPVHSTAFRPIDDASLARNPFRVFTSLLRLELIENEILRQKAAEILRQRDIFTPRCR
                     QLLEEYEQQGGFNETQAQEFVQEALETFRWHQSATVDEETYRALHNEHRLIADVVCFP
                     GCHINHLTPRTLDIDRVQSMMPECGIEPKILIEGPPRREVPILLRQTSFKALEETVLF
                     AGQKQGTHTARFGEIEQRGVALTPKGRQLYDDLLRNAGTGQDNLTHQMHLQETFRTFP
                     DSEFLMRQQGLAWFRYRLTPSGEAHRQAIHPGDDPQPLIERGWVAAQPITYEDFLPVS
                     AAGIFQSNLGNETQARNHGNASREAFEQALGCPVLDEFQLYQEAEERSKRRCGLL"
BASE COUNT         1902 a         1814 c         1704 g         1815 t
ORIGIN      
        1 gccgcttcgt gttgtggcaa aaaaagtgtt ggtgaaaata aatttttcag ccccttttca
       61 gacgacatag tcaatgccga aaaaggggct aagtcaatca tcagtagtta aatttcagta
      121 agttaagctg ctaaaaaact tcgactactg tgattaattt cacatccctt cgataaaaaa
      181 tatgcttacc gaaaagaaga acagtaaatc atctcagcgt ggggaatttt cacgcgcctt
      241 attcttagcg ggtcattttt tacgtgccag catcgtggcg aaacgcagtt taatacgatt
      301 accgttggcg tcggtgcggt gcagctcgcc gacgtcttca ttgtatttca ccctctccca
      361 gccttcgtaa tatcgacgta attctccctc tttgaaggca aacgggaagc cgacggtaca
      421 tggataatca gcggtatcca tcgccgccac aatcaggttg taaccaccag gtttagtgca
      481 acgttgcata ttggcaatca gccctgggat ggttttagcc tcaaggaaca tcagtaccac
      541 agtcgaaaga ataaaatcgt actgtctatc aaatgtgagg ttattcagat caacgactcg
      601 ggtgtgtaaa ttatccagat tttcaatgga tttaatgcgc tctacattgg cgatactcat
      661 ggcattttta tcccatgcgt caacatcata accattggct gccaggtaaa gactgttacg
      721 accattgcca cagcccagat ccagcgtttt acccggttta accactttca ccgcttccag
      781 tacttcagag tgtgtgcggg ttaattcata tttatcagta aaatagtttt cgtcacgaat
      841 gatcattctt tgtcctctgc tttcattaaa acggcgcgct cggttctgac taacaatttt
      901 ccctgcatca gaagcgcaaa agtacggatg agcagtattg caataataaa attggtaaag
      961 ataaacagcg gtaccgccag cgtatggaaa aatccatttt cgctgccact ccccagatgc
     1021 aaaccggtgg ttgccagtgc agatacgccg aacgagaaac tccagaatga agcattaaat
     1081 ggctgggaga gataccatgg catcagacgt agcataaaca gcagttgcag cagtccataa
     1141 ccaaaaagca ttttcgccag cgtatcaccc tcgccgccgt tgacgctcaa ccaggcacta
     1201 caggccacca gcgcaggagc gagctgaatg ccgagtgatg tccgcagtgc cgtgggtaat
     1261 tctcccgaac tgcgcagacg ctgcaagatc acaggttcaa ggcttagcca tgagaaaacg
     1321 cctgcgccta aaaacaccag accggcgtcg gtgtagccca acgcaccaca ggccattgcg
     1381 ctgataaagt tgttggcaac tgtcggcaga tacagtccag gcgtggtagc ttcttcaggg
     1441 tgagatccgc gccataatcc cgcagtttgc caggcggcat aagccaactg aaccacgaca
     1501 ccaaaactga acaggcacac cgccagtggg cgaaaccacg gaacaaaacc aatcgccacc
     1561 agcatcgtcg ttgccggaaa caaactcaca aaactgctca gcactggatg gcgaacttcc
     1621 gccagcacgc tatgcggaaa gcgtatgagt cgggtaataa atgcgctagt taataatccc
     1681 cagatgatca tcgccagaat caccagccca tcccctaacc agtggctgac ctgccaaacc
     1741 tggctggcat agcgccaggc aaatcccatc cctatcgtcc ccaacacaat accaaagtag
     1801 cctgccggca aattgagcac tttatcgctc tgcatcttct tactcatttt gtttaattta
     1861 taaagtatat ttgaaatgca ttttatggaa ttttctcgag catagccaga gccgcagaat
     1921 ttgctacggt tatagtaagt cacctgtggg aaaacgctat gcgcaaatat cgtctaagtg
     1981 aagaacagcg agcctttagt tatcaggaag atggcactaa aaaaaatgtg ttattacggc
     2041 agattatcgc cattagcgat tttaacgatg tgatagcagg aaccgctggc ggctggatcg
     2101 atcgcgaaac ggtgttagca caggaaggaa attgctggat ttacgaccag aatgctattg
     2161 cgttcggcgg aacggtgatt tccggtaata cgcgcatcac cggcaccagc gtactgtggg
     2221 gagaggttta cgcaacggat aatgtctgga tcgataatag tgaaatcagc cagggtgcat
     2281 atataagtga cggtgtcacc attcatgact cactggtttg cgggcaatgt agaatatttg
     2341 gtcacgcctt aattaatcaa cattctatga tcgtcgcagc acaaggctta acgcctgacc
     2401 atcaactcct gctacaaatt tatgatcgcg caagggttag cgcctcgcgt attgtccacc
     2461 aggcacaaat ttatggcgat gccgtcatcc ggtatgcttt tattgaacat cgtgccgaag
     2521 tttttgattt tgccagtatt gaaggtaatg aagagaacaa cgtctggcta tgcgattgcg
     2581 ctaaagtcta cggtcacgcg caggtgaaag ctggcataga agaagatgcc attccaacga
     2641 ttcattacag ttcgcaggtg gcggaatatg ccattgtgga aggtaattgt gtgttaaaac
     2701 atcatgtctt gattggcgga aatgccgtgg tacgtggtgg accgattctg ctcgacgagc
     2761 acgttgtaat acaaggtgag agccgtatta caggtgcggt gataattgaa aaccatgtgg
     2821 agttaaccga ccacgccgtc gttgaagcct ttgatggcga cacggttcat gtccgtggtc
     2881 caaaagtcat taacggcgag gagcgcatta cgcgaacgcc gctggcaggg ttattatgaa
     2941 tcgataatac gcgcgtataa gttcacatca tcataggcat cattcaggaa ctcagcctgt
     3001 ttcaggcaac cttcaaggat aaaaccattg cgcaaagcga cctggttgct ttgcggattg
     3061 tccacccgac atttgatcac gaagcgtcta agttcaccag actgggcgta atgatgaatc
     3121 aatgcctgca gcgcctgaga aatgatcccc tgcccctgat gagattcgtc cagccagtag
     3181 cctatttcag cggttttatt cagtggttca atacgattaa acgagataac gccgataagt
     3241 tcatcttctt tgaaaatcat gaacattttg gcatagccgc gttgatgcaa catcacatta
     3301 ccctgcaccg tttttcgcgt gtcctcttca ctctgaacga actgcggcca gtttagcgac
     3361 tgctgtaacc aggttttatt tttacagatt aactgataaa gaggagtgac gtgattttct
     3421 gcaacagcat gtaattcaag tgattcgctt acttttatcg tttcagtcat cttatctcca
     3481 gtcaatccat gcagttgtta aaaaagccgc ctggctggcg gcttaggaaa gtttacacct
     3541 ctttgacgct ctcctgctgc aggattttga gcatctcatc ttttaactgt agcttttgtt
     3601 ttttcatgcg gaccacttcc gcattgtacc ctcgaccgtc ggaaccttcc tttctggcaa
     3661 tttcatgatc aagtttattg tgtttatcga acaaggacat aaagcgagga ttttcgtttt
     3721 tcagacggga tattaaatct cgatattctg gaaacatacg cactccctgt tagtggctga
     3781 caaggtgtat ctgaaataca caccatcaga ttactcattt aacttaggga aaaatgcgag
     3841 cgggatcgtg tttaaccaga cggcacctcc cgaagaaggt gccgaagaaa aaacgattaa
     3901 ctcatcacgc ggtcgtcaac atactgacgt ttatccggcg ctggcgggaa atactgatac
     3961 agccatgttt cactgatagc gtcgccctga caacgcagat acatccgcat atcgaccgga
     4021 tcagtggagt ccgaagtcgg ataccagtca aactgaatac gataaccatc aatgggttca
     4081 atatagagaa tttcgatttg cttcgcttcc ccactggaaa gcgtaatcac cggctcaatg
     4141 ccttttggtg cggcagcttt cagatcacca ccaacgaaat cgacggcaaa acgacgcgcc
     4201 catttttcgg gatagtgttc acctggtgcc caaccttccg ggaaaccgcc catgccggta
     4261 cgcgtcgcca taacgcgcgc taatgggcaa tgaacaggcg gttgcgcact ccagtacaga
     4321 cgatactgga atgcaaactc atcacccgcc tttacagctt tttctggctg ccagaagcag
     4381 acaatgttat ccagcgtttc gcccgttgtt gggatttcca tcaggccgat ggtgccctta
     4441 ccccacttgt tacgcggttc cacccacaga cttgggcgtt tgttatacca gcccataatg
     4501 tcctgataat gggagaaatc acgatccagt tgcaataaac caaacccttt cgggttgttg
     4561 tcggtgtaag cattgaactg caatttttgc ggattattca gcggacggca aatccactcg
     4621 ccgttgcccc gccacatgga cagacgatca gagtcatgaa tttgcggatg aatggtgtcg
     4681 cacatccgac gttcattagt accgcagctg aacatactgg tcatcggcgc aatgcccagc
     4741 tgtttaatgt ctttgcgcgc atacaggtga ttttccacat ccataatcac ctgacttttc
     4801 tcacaatgga tagtgaactt ataggcacca gtaatgctgg cgctatcgag caacgcataa
     4861 acggtaaagg tagttgcccc cggttttacc gtatcaaacc agaaggcggt aaagtcgggg
     4921 aactcttctt tactgtcggt gtaagtgtca atcgccaggc cgcgggccga caaaccgtat
     4981 tgatatgtgt catcaacggc gcggaaataa ctcgctccga ggaatgatac tacatcacgg
     5041 cgcgccagtt cgggggcttt aaacacgcga aaaccggcaa agccgagatc gctttgccct
     5101 tctaattgtt tggtatcaac acctgcatcg ttgtatttga acaactccgg gcgaaagtga
     5161 atttcacgcg ccagatgtgt tgctggatct acagaaaaca tacgaacgcg gcgacggaat
     5221 cccattccca tatggaagaa ctgagcgtcc agttgacggt tctcaacgtt atgccagagc
     5281 gatttttcgg cgtcgtattg aatactgtta taagcctgcg gcgtcattgt cgccagcgtg
     5341 tcaggtaacg gacgaggcgc accacgccac gctgtttgcg ctaagtcgtg cgccattgac
     5401 tgtagaatgg agaagtcaaa acgctgggtt tgcccgtcgg caatatcaga atctgccgcg
     5461 aatgccgcct gagaaaaaag agaagcaatg ccgctggtac cgcacacggc ggccatagcc
     5521 attgaacctt taataaatcg tctacgatcc atacctgaaa gtgagtcctt ctggtgttgt
     5581 gaatggtccc gcgctggtcg ttttcagcgg caagaaagtg cttatttatg accgctcact
     5641 ctagacaata tttattaata atccaattgt tgaactcaga gaatatgcat aaatcggcaa
     5701 tttttttatg tcgatgggat gagcctgatt cgtttgtaaa atcaggcaaa tttttgcaaa
     5761 ggcggatggt cagaatttaa agcaaaccac agcgacgttt actgcgttct tccgcttcct
     5821 gataaagctg gaactcatcc aaaaccggac aacccaacgc ctgctcaaat gcctcgcgac
     5881 tggcattacc gtgattgcgt gcctgcgttt cattacccag atttgactgg aagatccccg
     5941 ccgcgctaac gggcaagaaa tcttcatagg taatgggttg cgccgcgacc caaccacgtt
     6001 caattaaggg ctgtggatcg tctccgggat gaatcgcctg acgatgcgcc tcacctgaag
     6061 gcgtcagacg gtaccggaac catgccagcc cttgctgacg cattaaaaac tcactgtcag
     6121 gaaaagtgcg gaaggtttcc tgtaaatgca tttggtgagt gagattatcc tgcccggttc
     6181 cagcgttacg cagaagatca tcatacagtt gtcgcccttt cggcgttaat gccacgccac
     6241 gctgctcaat ttcaccaaag cgcgcggtat gcgtgccctg tttctgcccc gcaaacaaca
     6301 ccgtctcttc cagtgcttta aagctggtct ggcgtagtaa aatcggtacc tcgcggcgcg
     6361 gcggcccctc gatgagaatt ttgggttcaa ttccgcattc aggcatcatc gactgcaccc
     6421 ggtcaatatc cagcgtacgt ggcgtcaggt ggttgatatg gcatccagga aaacagacca
     6481 catcagcaat caaccgatgt tcgttgtgca aggcgcgata ggtttcttca tctaccgttg
     6541 ctgactggtg ccagcgaaac gtttccaggg cttcctgcac aaactcctgt gcctgtgttt
     6601 cgttaaaacc gccctgctgc tcatattcct ctaacagttg tcgacaacgt ggggtgaaga
     6661 tatcgcgctg acgcagaatc tccgccgctt tctggcgcaa aatttcgttc tcgataagct
     6721 caaggcggag taaggaggta aaaacgcgga agggattacg cgccagagaa gcatcatcaa
     6781 tgggccgaaa tgctgtcgaa tgtaccggca cccctgcctg cgagagatcg taatagctca
     6841 ccgggtacat ccccataatg gcaaacatcc gccgaagagt agcaagctct tgtgcagtcc
     6901 caacgcgaat cgccccatga cgttcaacat tcagtcgcgc cagctcgtct gcatttacca
     6961 ttttttcgtg cagttgagga ttgttttcca gcacagccag attcacatca gctaccagtt
     7021 ccagcagcgt gccatattgc ggaacttctt gctggtacat ggctgacatt gcctgcgaaa
     7081 actgttcccg aatctcatcc gccgtgatgc tgttcgccat agtgtctgcc tccagtggtc
     7141 agtaatctgg agtgtaggta accgcattca ctctggcggg aagaatttac aaactgtgat
     7201 ctcgccgcga aaacatcaat attatccatt ttgct
//
LOCUS       DAZKHF020000216         7233 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2201S31241
            SAMN43267800-rid25106413.denovo.218, whole genome shotgun sequence.
ACCESSION   DAZKHF020000216 DAZKHF020000000
VERSION     DAZKHF020000216.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267800
            Sequence Read Archive: SRR30307263
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 7233)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7233)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 7233)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350581.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350581.2
            Genome Coverage       :: 151x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:21:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,652
            CDSs (total)                      :: 5,557
            Genes (coding)                    :: 5,320
            CDSs (with protein)               :: 5,320
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 237
            CDSs (without protein)            :: 237
            Pseudo Genes (ambiguous residues) :: 0 of 237
            Pseudo Genes (frameshifted)       :: 102 of 237
            Pseudo Genes (incomplete)         :: 130 of 237
            Pseudo Genes (internal stop)      :: 52 of 237
            Pseudo Genes (multiple problems)  :: 41 of 237
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7233
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267800-rid25106413.denovo.218"
                     /serotype="O 26:H11"
                     /isolate="2201S31241"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-01"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            complement(<1..114)
                     /locus_tag="ACHOSC_RS18720"
                     /old_locus_tag="pgaptmp_003744"
     CDS             complement(<1..114)
                     /locus_tag="ACHOSC_RS18720"
                     /old_locus_tag="pgaptmp_003744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708012.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4549598.1"
                     /translation="MKAFNKLFSLVVASVLVFSLAGCGDKEESKKFSANLNG"
     gene            complement(239..2239)
                     /gene="yehQ"
                     /locus_tag="ACHOSC_RS18725"
                     /old_locus_tag="pgaptmp_003745"
     CDS             complement(239..2239)
                     /gene="yehQ"
                     /locus_tag="ACHOSC_RS18725"
                     /old_locus_tag="pgaptmp_003745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708010.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SWIM zinc finger family protein"
                     /protein_id="HGU4549599.1"
                     /translation="MNSLRPELLELTPQALTALSNAGFVKRSLKELENGNVPEISHEN
                     GALIATFSDGVRTQLANSQALKEAQCSCGASGMCRHRVMLVLSYQRLCATVQPTEKEE
                     EWDPAIWLEGLATLPDATRKRAQALVAKGITIELFCAPGEIPSARLPMSDVRFYSRSS
                     IRFARCDCIEGTLCEHVVLAVQAFVEAKAQQAEFNHLIWQMRSEHVTSSDDPFASEEG
                     QTCRQYVQQLSQALWLGGISQPLIHYEAAFNRALQAAEACNWRWVSESLRQLRASVDA
                     FHTRASHYHAGECLRQLAALNSRLNCAQEMARRDSVGEVPPVPWRTVVGSGIAGEAKL
                     DHLRLVSLGMRCWQDIEHYGLRIWFTDPDTGSILHLSRSWPRSEQENSPAATRRLFSF
                     QAGALAGGQIVSQAAKRSADGELLLATRNRLSSVVPLSPDAWQMLSAPLRQPGIVALR
                     EYLRQRPPACIRPLNQVDNLFILPVAECISLGWDSSRQTLDAQVISGEGEDNLLTLSL
                     PASACSPFAVERMAALLQQTDDPVSLVSGFVSFVDGQLTLEPRVMMTKTRAWALDAET
                     APVAPLPSASVLPVPSTAHQLLMRCQALLIQLLHNGWRYQEQSAISQAELLANDLSAV
                     GFYRLAHVLGQFRNTESEARVEAMNNGVLLCEQLFPMLQQQG"
     gene            complement(2236..3372)
                     /gene="yehP"
                     /locus_tag="ACHOSC_RS18730"
                     /old_locus_tag="pgaptmp_003746"
     CDS             complement(2236..3372)
                     /gene="yehP"
                     /locus_tag="ACHOSC_RS18730"
                     /old_locus_tag="pgaptmp_003746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HGU4549600.1"
                     /translation="MSELNDLLTTRELQRWRLILGEAAETTLCGLDDNARQIDHALEW
                     LYGRDPERLQRGERSGGLGGSNLTTPEWINSIHTLFPQQVIERLESDAVLRYGIEDVV
                     TNLDVLERMQPSESLLRAVLHTKHLMNPEVLAAARQIVRQVVEEIMARLAKEVRQAFS
                     GVRDRRRRSLIPLARNFDFKSTLRANLQHWHPQHGKLYIESPRFNSRIKRQSEQWQLV
                     LLVDQSGSMVDSVIHSAVMAACLWQLPGIRTHLVAFDTSVVDLTADVADPVELLMKVQ
                     LGGGTNIASAVEYGRQLIEQPAKSVIILVSDFYEGGSSSLLTHQVKKCVQSGIKVLGL
                     AALDSTATPCYDRDTAQALVNVGAQIAAMTPGELASWLAENLQS"
     gene            complement(3365..5644)
                     /gene="yehM"
                     /locus_tag="ACHOSC_RS18735"
                     /old_locus_tag="pgaptmp_003747"
     CDS             complement(3365..5644)
                     /gene="yehM"
                     /locus_tag="ACHOSC_RS18735"
                     /old_locus_tag="pgaptmp_003747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5682 family protein"
                     /protein_id="HGU4549601.1"
                     /translation="MSEPLIVGIRHHSPACARLVKSLIESQRPRYVLIKGPADFNDRV
                     DELFLSHQLPVAIYSYCQYQDGAAPGRGAWTPFAEFSPEWQALQAARRIQAQTYFIDL
                     PCWAQSEEEDDSPDTQDESQALLLRATRMDNSDTLWDHLFEDESQQTALPSALAHYFA
                     QLRGDFPGDALNRQREAFMARWIAWAVQQNNGDVLVVCGGWHAPALAKMWRECPQDIN
                     TPELPSLADAITGCYLTPYSEKRLDVLAGYLSGMPAPVWQNWCWQWGLQQAGEQLLKT
                     ILTRLRQHKLPASTADMAAAHLHAMALAQLRGHTLPLRTDWLDAIAGSLIKEALNAPL
                     PWSYRGVIHPDTDPILLTLIDTLAGDGFGKLAPSTPQPPLPKDVTCELERTAISLPAE
                     LTLNRFNPNGLAQSQVLHRLAILEIPGIVRQQGSTLTLAGNGEERWKLTRPLSQHAAL
                     IEAACFGATLQEAARHKLEANMLDAGGIGSITTCLSQAALAGLASFSQQLLEQLTLLI
                     AQENQFAEMGQALEVLYALWRLDEISGMQGAQILQTTLCAAIDRTLWLCESNGRPDEK
                     EFHAHLHSWQALCHILRDLHSGVQLPGISLSAAVALLERRSQAIHAPALDRGAAHGAL
                     MRLEHPNASAEAALTMLAQLSPAQSGEALHGLLALARHQLACQPTFIAGFSSHLNQLS
                     DADFINALPDLRAAMAWLPPRERGTLAHQVLEHYQLAQLPVSALQMPLHCPPQAIAHH
                     QQLEQQALASLQNWGVFHV"
     gene            complement(5655..6741)
                     /gene="yehL"
                     /locus_tag="ACHOSC_RS18740"
                     /old_locus_tag="pgaptmp_003748"
                     /pseudo
     CDS             complement(5655..6741)
                     /gene="yehL"
                     /locus_tag="ACHOSC_RS18740"
                     /old_locus_tag="pgaptmp_003748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310954.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
BASE COUNT         1692 a         1955 c         1937 g         1649 t
ORIGIN      
        1 gccgttcaga ttggcgctga atttcttcga ttcttctttg tcaccgcagc cagcaagaga
       61 gaaaaccaga acagatgcaa caacgaggga aaacagctta ttgaaagcct tcatgtaaaa
      121 ctccatttta tttaatcaag aaactggtga ctctcaccag gggctatata gaatatgcct
      181 aataccgtga cgtgagcagt ccggaactgg agtagaactc ttagtaaaaa gcactatttc
      241 atccttgttg ctgaagcatg gggaataatt gttcgcaaag taaaacaccg ttattcattg
      301 cttctacccg tgcctcgctt tctgtattac gaaattgtcc caacacatgt gccagccgat
      361 aaaaaccgac cgcggagagg tcattcgcca gcaactctgc ctgactaata gcgctctgtt
      421 cctgatagcg ccagccgtta tggagcagtt gaataagtaa cgcctggcag cgcatcagca
      481 actgatgagc ggtagacggc acaggcaaaa cgctggcaga aggtagcggt gccacaggcg
      541 cagtttccgc gtccagcgcc caggcgcggg tttttgtcat catcacccgt ggttccagtg
      601 tcaattgccc atcaacaaaa ctgacaaagc cagaaaccag actcacgggg tcgtctgttt
      661 gttgcaaaag cgccgccatg cgttcaacgg caaaaggaga acaggcagat gccggaaggg
      721 ataacgtcag gagattatcc tcaccttcgc cgctgattac ctgcgcatcc agcgtctggc
      781 ggctgctatc ccaaccgagc gaaatacact cagcgaccgg cagaataaat aagttatcga
      841 cctgattaag aggccgtatg caggcggggg gacgctggcg taaatattcc cgcaaagcca
      901 caatgcccgg ctggcgtaac ggcgcgctca acatttgcca ggcatcaggc gacagcggca
      961 caacgctgct taagcggttg cgggtagcta acagcagctc gccatcggca ctgcgttttg
     1021 ctgcttgtga aacaatttgc ccacccgcca gtgcgccagc ctgaaaacta aacagccgac
     1081 gcgtagctgc cggtgagttt tcctgttcac ttcgcggcca actgcgcgaa aggtgcaaaa
     1141 tactgccggt gtcgggatcg gtaaaccaga tgcgtaaacc ataatgctca atatcctgcc
     1201 agcaacgcat acctaaagac accagccgca gatgatcaag ctttgcttct ccggcaatgc
     1261 cagagccaac gaccgtgcgc cacggcacag gaggaacttc accaacactg tcgcgccggg
     1321 ccatctcttg tgcgcaattt aatcgactgt ttaatgccgc aagctgacgt aagcattctc
     1381 cggcatgata gtggctggcg cgagtgtgga aggcatcaac gcttgcccgt agctgtcgta
     1441 gtgattcact cacccagcgc cagttgcagg cctctgccgc ctgcaatgcg cggttgaatg
     1501 ctgcctcgta gtgaataagc ggctggctga tgccgccaag ccataatgcc tggcttaatt
     1561 gctgaacata ttgacgacac gtttggcctt cttcgctggc aaacggatcg tcagatgatg
     1621 tgacgtgttc gctgcgcatc tgccagatta aatggttaaa ttctgcttgc tgcgctttgg
     1681 cctcgacgaa ggcctgcaca gccagtacga catgttcgca aagtgtgcct tcaatacaat
     1741 cacaacgggc gaaacgaata ctgctacggg aataaaaacg cacatcgctc atcggtaagc
     1801 gggcagaggg aatttcgccc ggcgcacaga acaactcaat ggtgatgcct ttagcgacca
     1861 gcgcctgcgc gcgtttgcgg gtggcatcgg gcagggtagc cagtccttcc agccagattg
     1921 ccggatccca ctcttcttct ttttccgtgg gctggacggt ggcacaaagt cgttgataac
     1981 ttaacaccag catcacgcga tgacggcaca tgccgctggc cccgcagctg cactgagcct
     2041 ctttcagtgc ctggctgttc gccagctggg tacggacacc gtcactgaag gtggcgatta
     2101 aagcgccgtt ctcatggctg atttccggga cgttgccatt ttccagttcc ttaagactgc
     2161 gcttaacaaa accggcattg cttaacgccg tcagtgcctg cggtgtcagt tctaataatt
     2221 ccggacgtag tgaattcatg actgaagatt ctccgcaagc catgatgcca gctcgcccgg
     2281 cgtcatggcg gctatttgtg cgccgacatt aaccagcgcc tgggccgtat cgcggtcata
     2341 gcaaggcgtt gcggtgctat cgagcgctgc cagtcccagc actttgatgc cgctctggac
     2401 acactttttc acctgatgcg tcagcaatga tgatgaaccc ccttcgtaaa aatcgctcac
     2461 gaggataatg acgctttttg ctggttgttc aataagttgc cgaccatact ccacggcact
     2521 ggcgatattg gtcccgccgc ccaactgtac tttcattaat aactctaccg gatcggcaac
     2581 gtctgccgtg agatcaacca cgcttgtgtc aaacgccacc agatgggtac gaatgccggg
     2641 taactgccac aaacaggccg ccatcaccgc agagtggatc accgagtcca ccatcgatcc
     2701 gctttgatca accagtaaga ccagttgcca ttgttcgctt tggcgtttaa tgcggctgtt
     2761 aaagcggggg gattcgatat acaacttgcc gtgttgcggg tgccagtgtt gcaggttggc
     2821 gcgcagagta cttttgaaat caaagtttcg cgccagtgga attaatgagc ggcgacggcg
     2881 atcgcggaca ccagaaaaag cctgacgaac ttcctttgcc agtcgcgcca taatttcttc
     2941 aacaacctgg cgcactatct ggcgggcggc agccagtact tcgggattca tcagatgttt
     3001 ggtgtgcaaa acggcgcgta gcaggctttc cgaaggctgc atacgttcca gcacgtcgag
     3061 attcgtcacc acatcttcaa tgccgtagcg cagtacggca tcgctttcca gtcgctcaat
     3121 cacctgttgc ggaaacagag tgtgaatact gttgatccac tcaggggtgg tgagatttga
     3181 gccacctaat ccaccagagc gttcaccacg ctggagccgt tcaggatcgc gcccatacag
     3241 ccactccagc gcgtggtcta tctgccgggc gttgtcatcc agcccacaaa gcgtcgtttc
     3301 tgccgcttcg ccaagaatta atcgccagcg ttgtagctca cgggtggtca gaagatcgtt
     3361 cagttcagac atggaaaact ccccagtttt gcagcgatgc cagtgcctgc tgttcgagtt
     3421 gttgatgatg tgcaatggcc tgtggtggac aatgcaacgg catttgcagc gccgaaacgg
     3481 gaagttgcgc cagttgataa tgctcaagca cctgatgcgc cagcgtcccg cgttctcgtg
     3541 gtggtagcca ggccatcgcc gcgcgtaaat cgggcagggc attgataaaa tcggcatcac
     3601 ttagttgatt taaatgactg ctgaaaccgg cgataaatgt cggctgacag gccagttgat
     3661 ggcgggccag cgccagcaaa ccgtgcagcg cttcgccgga ttgtgccggg gataactgcg
     3721 ccagcatcgt cagcgccgct tcggcactgg cgttaggatg ttccagacgc atcagtgcgc
     3781 catgagccgc gccgcgatcc agcgccgggg catgaattgc ctgactgcgt cgctccagta
     3841 aggctaccgc cgcagaaaga gaaatgccgg ggagttgcac accgctatgt agatcgcgca
     3901 ggatatgaca caacgcttgc cagctatgca ggtgagcgtg aaactccttt tcatctggtc
     3961 tgccgttaga ttcacatagc cacagcgtgc gatcgatagc cgcgcataac gtcgtttgta
     4021 atatctgcgc gccttgcata ccgctaattt catccagccg ccataaggca tatagcactt
     4081 ccagcgcctg gcccatttcg gcaaattgat tttcctgggc gattaataat gtgagttgct
     4141 ccagtaattg ttgactgaag gacgccagac ccgctaacgc cgcctggcta agacatgtgg
     4201 tgatactgcc gattccaccc gcgtccagca tattggcttc taatttatgg cgtgcggctt
     4261 cctggagtgt tgcaccaaag caggcggcct caatcaatgc cgcatgctgg ctaagcgggc
     4321 gggttaattt ccagcgttct tcaccgttgc ctgcaagtgt cagtgtgctt ccctgctggc
     4381 gtacaatccc tgggatctcc agtattgcca gccgatgtaa cacctgactt tgcgctagtc
     4441 cattggggtt aaagcgattt aaggtaagct ccgccggaag agagattgcg gtacgttcca
     4501 gttcgcaggt gacatctttt ggcagaggcg gttgtggcgt agaaggggca agtttaccga
     4561 atccgtcacc cgctaatgtg tctatcaacg ttagcagaat cggatcggta tcgggatgaa
     4621 taacgccgcg atagctccac ggcaacggcg cattcagggc ttctttaatc agcgagcctg
     4681 ctatggcatc cagccagtca gtgcgtaacg gtagtgtatg accgcgcaac tgtgccagcg
     4741 ccatcgcatg cagatgagcg gcagccatat ccgcggtcga agcaggcaac ttgtgctggc
     4801 gcaaacgggt aaggatagtt tttagcagtt gttcaccggc ctgctgtaag ccccactgcc
     4861 agcaccagtt ttgccagacc ggggcaggca taccggaaag gtatcctgcc agcacatcaa
     4921 ggcgcttttc actgtagggc gtgagataac aacctgtaat ggcatctgcc agcgagggca
     4981 attccggcgt gttaatgtcc tgcgggcatt cgcgccacat ttttgccagt gccggagcgt
     5041 gccagccacc gcagacgact aacacgtcgc cattattttg ctgcaccgcc catgcaatcc
     5101 agcgagccat aaaggcttcg cgctgacgat tgagtgcatc gccggggaaa tcgccccgca
     5161 gttgggcaaa atagtgcgcc agcgcagagg gtaatgcagt ttgctggctt tcatcttcga
     5221 acaagtgatc ccacagggta tcgctgttat ccatgcgggt ggcacgcagc agtaaggcct
     5281 ggctttcatc ttgcgtatca ggcgaatcgt cctcttcttc actctgcgcc cagcaaggca
     5341 aatcgatgaa gtaagtttgt gcctgaatac gacgtgcggc ttgtagcgcc tgccactccg
     5401 gcgaaaattc agcaaatggc gtccaggcac cacgcccggg ggctgcaccg tcctgatact
     5461 ggcaataact gtaaatagct accggaagct ggtgggataa aaacagttcg tctacccggt
     5521 cattaaaatc agccgggcct ttaatcaaca cgtatcgtgg ccgctggctt tcgattaacg
     5581 atttcaccag ccgggcgcag gccggactat gatgccggat gccgacaatt aacggctcgc
     5641 tcatgcatct ttcctcacgg caggcggtgg cgggcttgat aataagcctg ccagtgagct
     5701 tctttatgtg tggcgactcg ttgttcgaag taacggcgca gacgagcgcg atcttcttcg
     5761 ttatctttga cgatggttcc ggcaatgcag tccaccaggt cggctggctc acctgcgcga
     5821 ttcgctaaaa accaggcgcg gacgcctacg gcgtgggcca cgttcacggc ttcagcggtg
     5881 gacataatcg ctgttagtgt atccattgac gttttctttt cgccgttggc acgcagatcg
     5941 cggaaggtgc ggaccagtaa ctccagtacc gcgtcaggca ctttatgcgg aataccgcta
     6001 tgggccagca aacgcgccga agcactggcg acaagctcca gctcctgagc gaaatccata
     6061 atcggaaaga cagtttcaaa atcgaagcga cgctttagcg cggcactcat ctcattgacg
     6121 ccgcgatcgc gggtattggc agtggcaata atattaaagc cttcccgcgc atagagttga
     6181 ctggcttcac cagtgagttc cggcacccat aactctgtca gaaagcatac ctaacagaca
     6241 atcttgcact tccagcggtg tacgcgttat ctcttcgaaa cggacgattt tgccgtcgcg
     6301 cattccctgg taaagcgggg cagggaccag cgcttccgtt gatgggccat gattgataag
     6361 cagagcgtag ttccagccgt acttgatttg atcttcagtg gtagatgcgc cgccctggat
     6421 ggttaacccg gcatcgccgc taatagcggt tgccagtaat tcagacagga gagattttgc
     6481 ggtgccaggt tcccccacca acatcaaacc gcgtccggtg gcaagagtga ctaacatacg
     6541 ttcgacagcg gagggactaa tcaccacttt acggctaata ttttgcgcgc tatcgccaag
     6601 gataaaagca cgggccgcag gcaaacttaa ctgccaaccg ggcgggcaag gtgcgttatc
     6661 attttgtttt aattttgcca gttcatcggc gtataacaca gcagcaggcg gacgctgcag
     6721 atgattattc tgcggtgaca tatattcatc cctgaaatgt attaatgaaa taaacgatat
     6781 tttacgcgca ataaatgttt ggcgcattag ttgtttgagc ggaaaatatc tggaaagcag
     6841 atcacagtaa ataaaaaaac ctgctggtga ggcaggttct tttatttact attttcaggc
     6901 ctaacaccac agcctgtgca acatcgtgtt ccaacgaacg aattataacc acggctggac
     6961 cccgttgcaa ttaagataat gagatttata atcctgactg ttgggacgtt gcgccggatc
     7021 gaacctattt caagtcctgg ctaccggacg gggggtgctg caggtggtgc cctgatccgg
     7081 agtgcaacat cgtggtccaa catacgtaaa ccagtatcga caggcagtgt aagggtaagg
     7141 tgctggtgtt gcgctcctta ataaggagag cggatggact cgctgacaca aaagttaacc
     7201 gtgctcattg ctgtactgga gctattagtg gct
//
LOCUS       DAZKHF020000217         7219 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2201S31241
            SAMN43267800-rid25106413.denovo.219, whole genome shotgun sequence.
ACCESSION   DAZKHF020000217 DAZKHF020000000
VERSION     DAZKHF020000217.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267800
            Sequence Read Archive: SRR30307263
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 7219)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7219)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 7219)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350581.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350581.2
            Genome Coverage       :: 151x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:21:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,652
            CDSs (total)                      :: 5,557
            Genes (coding)                    :: 5,320
            CDSs (with protein)               :: 5,320
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 237
            CDSs (without protein)            :: 237
            Pseudo Genes (ambiguous residues) :: 0 of 237
            Pseudo Genes (frameshifted)       :: 102 of 237
            Pseudo Genes (incomplete)         :: 130 of 237
            Pseudo Genes (internal stop)      :: 52 of 237
            Pseudo Genes (multiple problems)  :: 41 of 237
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7219
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267800-rid25106413.denovo.219"
                     /serotype="O 26:H11"
                     /isolate="2201S31241"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-01"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            complement(77..781)
                     /locus_tag="ACHOSC_RS18745"
                     /old_locus_tag="pgaptmp_003749"
     CDS             complement(77..781)
                     /locus_tag="ACHOSC_RS18745"
                     /old_locus_tag="pgaptmp_003749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Bax inhibitor-1/YccA family protein"
                     /protein_id="HGU4549602.1"
                     /translation="MDRFPRSDSIVQPRAGLQTYMAQVYGWMTVGLLLTAFVAWYAAN
                     SAAVMELLFTNRVFLIGLIIAQLALVIVLSAMIQKLSAGVTTMLFMLYSALTGLTLSS
                     IFIVYTAASIASTFVVTAGMFGAMSLYGYTTKRDLSGFGNMLFMALIGIVLASLVNFW
                     LKSEALMWAVTYIGVIVFVGLTAYDTQKLKNMGEQIDTRDTSNLRKYSILGALTLYLD
                     FINLFLMLLRIFGNRR"
     gene            complement(918..1370)
                     /gene="moaE"
                     /locus_tag="ACHOSC_RS18750"
                     /old_locus_tag="pgaptmp_003750"
     CDS             complement(918..1370)
                     /gene="moaE"
                     /locus_tag="ACHOSC_RS18750"
                     /old_locus_tag="pgaptmp_003750"
                     /EC_number="2.8.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin synthase catalytic subunit MoaE"
                     /protein_id="HGU4549603.1"
                     /translation="MAETKIVVGPQPFSVGEEYPWLAERDEDGAVVTFTGKVRNHNLG
                     DSVKALTLEHYPGMTEKALAEIVDEARNRWPLGRVTVIHRIGELWPGDEIVFVGVTSA
                     HRSSAFEAGQFIMDYLKTRAPFWKREATPEGDRWVEARESDQQAAKRW"
     gene            complement(1372..1617)
                     /gene="moaD"
                     /locus_tag="ACHOSC_RS18755"
                     /old_locus_tag="pgaptmp_003751"
     CDS             complement(1372..1617)
                     /gene="moaD"
                     /locus_tag="ACHOSC_RS18755"
                     /old_locus_tag="pgaptmp_003751"
                     /EC_number="2.8.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin synthase sulfur carrier subunit"
                     /protein_id="HGU4549604.1"
                     /translation="MIKVLFFAQVRELVGTDATEVAADFPTVEALRQHMAAQSDRWAL
                     ALEDGKLLAAVNQTLVSFDHPLTDGDEVAFFPPVTGG"
     gene            complement(1610..2095)
                     /gene="moaC"
                     /locus_tag="ACHOSC_RS18760"
                     /old_locus_tag="pgaptmp_003752"
     CDS             complement(1610..2095)
                     /gene="moaC"
                     /locus_tag="ACHOSC_RS18760"
                     /old_locus_tag="pgaptmp_003752"
                     /EC_number="4.6.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0061799 - cyclic pyranopterin
                     monophosphate synthase activity [Evidence IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic pyranopterin monophosphate synthase MoaC"
                     /protein_id="HGU4549605.1"
                     /translation="MSQLTHINAAGEAHMVDVSAKAETVREARAEAFVTMRSETLAMI
                     IDGRHHKGDVFATARIAGIQAAKRTWDLIPLCHPLMLSKVEVNLQAEPEHNRVRIETL
                     CRLTGKTGVEMEALTAASVAALTIYDMCKAVQKDMVIGPVRLLAKSGGKSGDFKVEAD
                     D"
     gene            complement(2098..2610)
                     /gene="moaB"
                     /locus_tag="ACHOSC_RS18765"
                     /old_locus_tag="pgaptmp_003753"
     CDS             complement(2098..2610)
                     /gene="moaB"
                     /locus_tag="ACHOSC_RS18765"
                     /old_locus_tag="pgaptmp_003753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum cofactor biosynthesis protein B"
                     /protein_id="HGU4549606.1"
                     /translation="MSQVSTEFIPTRIAILTVSNRRGEEDDTSGHYLRDSAQEAGHHV
                     VDKAIVKENRYAIRAQVSAWIASDDVQVVLITGGTGLTEGDQAPEALLPLFDREIEGF
                     GEVFRMLSFEEIGTSTLQSRAVAGVANKTLIFAMPGSTKACRTAWENIIAPQLDARTR
                     PCNFHPHLKK"
     gene            complement(2632..3621)
                     /gene="moaA"
                     /locus_tag="ACHOSC_RS18770"
                     /old_locus_tag="pgaptmp_003754"
     CDS             complement(2632..3621)
                     /gene="moaA"
                     /locus_tag="ACHOSC_RS18770"
                     /old_locus_tag="pgaptmp_003754"
                     /EC_number="4.1.99.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP 3',8-cyclase MoaA"
                     /protein_id="HGU4549607.1"
                     /translation="MASQLTDAFARKFYYLRLSITDVCNFRCTYCLPDGYKPSGVTNK
                     GFLTVDEIRRVTRAFASLGTEKVRLTGGEPSLRRDFTDIIAAVRENDAIRQIAVTTNG
                     YRLERDVANWRDAGLTGINVSVDSLDARQFHAITGQDKFNQVMAGIDAAFEAGFEKVK
                     VNTVLMRDVNHHQLDTFLNWIQHRPIQLRFIELMETGEGSELFRKHHISGQVLRDELL
                     RRGWIHQLRQRSDGPAQVFCHPDYAGEIGLIMPYEKDFCATCNRLRVSSIGKLHLCLF
                     GEGGVNLRDLLEDDTQQQALEARISAALREKKQTHFLHQNNTGITQNLSYIGG"
     regulatory      complement(3609..3753)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01055"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="molybdenum cofactor riboswitch; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /bound_moiety="molybdenum cofactor"
                     /db_xref="RFAM:RF01055"
     gene            4018..4926
                     /gene="yvcK"
                     /locus_tag="ACHOSC_RS18775"
                     /old_locus_tag="pgaptmp_003755"
     CDS             4018..4926
                     /gene="yvcK"
                     /locus_tag="ACHOSC_RS18775"
                     /old_locus_tag="pgaptmp_003755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uridine diphosphate-N-acetylglucosamine-binding
                     protein YvcK"
                     /protein_id="HGU4549608.1"
                     /translation="MRNRTLADLDRVVALGGGHGLGRVLSSLSSLGSRLTGIVTTTDN
                     GGSTGRIRRSEGGIAWGDMRNCLNQLITEPSVASAMFEYRFGGNGELSGHNLGNLMLK
                     ALDHLSVRPLEAINLIRNLLKVDTHLIPMSEHPVDLMAIDDQGHEVYGEVNIDQLTTP
                     IQELLLTPNVPATREAVHAINEADLIIIGPGSFYTSLMPILLLKEIAQALRRTPAPMV
                     YIGNLGRELSLPAANLKLESKLAIMEQYVGKKVIDAVIVGPKVDVSAVKERIVIQEVL
                     EASDIPYRHDRQLLHNALEKALQALG"
     gene            complement(5118..7139)
                     /gene="uvrB"
                     /locus_tag="ACHOSC_RS18780"
                     /old_locus_tag="pgaptmp_003756"
     CDS             complement(5118..7139)
                     /gene="uvrB"
                     /locus_tag="ACHOSC_RS18780"
                     /old_locus_tag="pgaptmp_003756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009380 - excinuclease repair complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrB"
                     /protein_id="HGU4549609.1"
                     /translation="MSKPFKLNSAFKPSGDQPEAIRRLEEGLEDGLAHQTLLGVTGSG
                     KTFTIANVIADLQRPTMVLAPNKTLAAQLYGEMKEFFPENAVEYFVSYYDYYQPEAYV
                     PSSDTFIEKDASVNEHIEQMRLSATKAMLERRDVVVVASVSAIYGLGDPDLYLKMMLH
                     LTVGMIIDQRAILRRLAELQYARNDQAFQRGTFRVRGEVIDIFPAESDDIALRVELFD
                     EEVERLSLFDPLTGQIVSTIPRFTIYPKTHYVTPRERIVQAMEEIKEELAARRKVLLE
                     NNKLLEEQRLTQRTQFDLEMMNELGYCSGIENYSRFLSGRGPGEPPPTLFDYLPADGL
                     LVVDESHVTIPQIGGMYRGDRARKETLVEYGFRLPSALDNRPLKFEEFEALAPQTIYV
                     SATPGNYELEKSGGDVVDQVVRPTGLLDPIIEVRPVATQVDDLLSEIRQRAAINERVL
                     VTTLTKRMAEDLTEYLEEHGERVRYLHSDIDTVERMEIIRDLRLGEFDVLVGINLLRE
                     GLDMPEVSLVAILDADKEGFLRSERSLIQTIGRAARNVNGKAILYGDKITPSMAKAIG
                     ETERRREKQQKYNEEHGITPQGLNKKVVDILALGQNIAKTKAKGRGKSRPIVEPDNVP
                     MDMSPKALQQKIHELEGLMMQHAQNLEFEEAAQIRDQLHQLRELFIAAS"
BASE COUNT         1721 a         1929 c         1829 g         1740 t
ORIGIN      
        1 tggtacttgt taagtgtcgt ttttagtaag aaaaaaaatc ccgacacatg gccgggatat
       61 tgatggtgaa aaagaattaa cggcggttgc caaaaattcg cagcaacatc aggaacaggt
      121 tgatgaagtc cagatacaag gttaacgcgc caagaatgga atatttgcgc aggttcgacg
      181 tgtcgcgggt atcaatctgc tcacccatat ttttcaattt ctgagtgtca tacgccgtca
      241 atccgacaaa gacaatcacg ccgatgtagg taactgccca catcaatgct tcgcttttca
      301 gccagaagtt gaccagcgat gccagcacaa tgccgattaa cgccataaac agcatattgc
      361 caaagccact taaatcgcgc ttcgtggtgt aaccgtacag gctcattgcg ccgaacatcc
      421 cggcagtaac gacgaaagta ctggcgatag aagcagcggt atagacaatg aatatactgg
      481 aaagcgtaag acccgtcagc gccgaataaa gcataaagag catcgtcgtt acacctgcgc
      541 tcagcttttg aatcatcgct gataacacaa taaccaatgc taattgcgcg atgatcagac
      601 cgattaaaaa gacacggtta gtaaacaaca gctccatcac ggccgcggaa ttagccgcat
      661 accaggcaac aaatgcggtc agcaacaagc caacggtcat ccagccatag acttgcgcca
      721 tataagtttg caagccagcc cggggttgta cgattgaatc agaacgtggg aatctgtcca
      781 tgacgatctc ctgaagatat aaggaatatc ttaaggatac tgcaaaatga tgaggctgtg
      841 catcgacgca gcgtaaacgc atgtactgag cggtgaaatt gccggacgca gcggtgcctt
      901 atccggctaa caaaaaacta ccagcgtttt gccgcctgct gatcgctctc ccgagcttca
      961 acccagcggt cgccttccgg cgtggcttcg cgcttccaga acggtgcgcg ggttttgaga
     1021 taatccataa taaactgccc ggcttcaaac gcactgctgc gatgcgcact ggtgacaccg
     1081 acaaaaacga tttcatcgcc cggccataat tccccgatgc ggtgaatcac agtgacgcgc
     1141 cccagcggcc agcggttacg cgcttcatca acaatttctg ccagtgcttt ttcagtcatc
     1201 cccggatagt gttcgagggt taatgctttg acgctgtcgc ccaggttatg gttgcgcacc
     1261 ttaccagtaa aggtgactac cgcaccgtct tcgtcacgct ccgccagcca cgggtactct
     1321 tctcctacgc tgaacggctg cggaccaaca acaattttgg tttctgccat cttaacctcc
     1381 ggttaccggc gggaagaaag ctacttcgtc gccgtcagtc agcggatggt caaaactcac
     1441 cagcgtctgg ttgacggcag ccagtaattt gccatcttcc agcgccagcg cccagcgatc
     1501 gctctgcgca gccatgtgct ggcgtaacgc ttcaacagtt gggaaatccg cagccacttc
     1561 ggttgcatct gttcccacca actcgcgcac ctgggcgaaa aaaagaactt taatcatccg
     1621 cttccacctt aaagtcaccc gacttgccgc cgctcttcgc cagcaaacgt accggaccaa
     1681 tcaccatatc tttttgcacc gctttgcaca tgtcataaat ggtcagcgcc gccacggagg
     1741 ccgcggttaa tgcttccatt tcgacaccgg ttttcccggt caggcggcat aaggtttcta
     1801 tacgcacccg attgtgctcc ggctcggcct gtaaattgac ttcaactttg ctgagcatca
     1861 gcggatgaca gagcgggatc agatcccagg tgcgttttgc cgcctgaata ccggcaatac
     1921 gcgcagtggc aaatacgtcg cctttgtggt ggcgaccatc aataatcatc gccagcgtct
     1981 cgctgcgcat ggtgacaaag gcttccgccc gcgcttcacg cacggtttcc gctttggcgg
     2041 agacatccac catgtgcgct tcgccagcgg cgttgatatg ggtcagttgc gacatactta
     2101 tttcttcaaa tgtggatgga aattacacgg acgcgtacgg gcatccagct gcggcgcgat
     2161 gatattttcc catgcggtac ggcacgcttt ggtcgaaccc ggcatggcga aaatcagcgt
     2221 tttgttggcg acgcccgcta ccgcacgaga ttgcaacgtg gaagtgccaa tctcttcaaa
     2281 cgacaacata cggaacactt caccaaaacc ttcaatttca cggtcgaaca acggcagcaa
     2341 tgcttcggga gcctgatcac cttccgtcag gccagtacca cccgtaatca ataccacttg
     2401 tacatcgtcg ctggcgatcc acgcagatac ctgagcgcga atagcgtagc ggttttcttt
     2461 cacaatggct ttatcgacaa cgtgatggcc cgcttcttgc gccgaatcgc gcagatagtg
     2521 accggaggta tcgtcttctt caccgcgacg attagaaacc gtaagaatag caatacgggt
     2581 cgggataaat tcagtgctta cctgactcat ctgatctctc cttttgacgt tttagccgcc
     2641 aatgtacgat aagttttgcg taataccggt gttgttttga tgcaggaaat gggtctgttt
     2701 cttctcccgc agcgccgctg aaatacgcgc ttccagcgcc tgttgctggg tatcgtcttc
     2761 cagcagatcg cgcaggttaa cgccgccttc accaaacagg cagagatgga gtttaccaat
     2821 ggaggaaacg cgcaggcggt tgcaagtggc gcagaagtct ttttcatacg gcatgataag
     2881 gccaatctct ccggcgtaat ctggatggca aaagacttgc gcgggaccgt cgctgcgttg
     2941 acgtaattgg tggatccagc cgcgacgcag tagctcgtca cgcagaacct gaccagagat
     3001 gtgatgctta cggaagagct cgctgccctc gcccgtttcc atcagttcga tgaaacgcag
     3061 ctggataggg cgatgctgga tccagttcag aaaggtgtcg agctggtgat gattaacatc
     3121 acgcatcagc acggtattga ctttgacctt ctcaaaaccg gcctcaaatg cagcatcaat
     3181 ccccgccatg acctggttga atttatcctg cccggtaata gcgtgaaact ggcgggcgtc
     3241 cagactgtcg acactgacgt taatgccagt aagtcccgca tcgcgccagt tcgccacatc
     3301 gcgttccaga cggtaaccat tggtggtgac cgcaatctgg cggatagcgt cgttttcccg
     3361 cacagcggcg atgatatcgg taaagtcgcg gcgtaaagac ggctcaccgc ctgtcaggcg
     3421 cactttttcg gtgcccagac tggcgaaggc gcgcgtaacc cggcgaattt catcgacggt
     3481 aagaaagcct ttattggtga cgccgctcgg tttgtagcca tccggcaggc agtaggtgca
     3541 acgaaagtta cacacatcgg taatcgacag gcgcaagtag taaaacttac gcgcaaatgc
     3601 atcagtcagt tgtgaagcca tgtacacctt tccagatacg ggaggcgaag tcatttcttc
     3661 cttcgccctg gtggcaatct ttacgagcat tgtcacggcc aaagcaccgt atcagttgac
     3721 ccaggtgcag aggctagagt gtttagtggt tatgccgata ctagcgtgta aatgagattt
     3781 ttaccatcca cactttcgct atgtaatcat gtatatagcg tcatgatcgt gctatttcgc
     3841 tacagaataa gggaaaatgg cggttttact ttgatataca tcatcaggga ggcatagcgt
     3901 gcatcgtcct ttagaggtag aagaaaatag tttgtatcct caatattggc aggttaattg
     3961 ctgtttcccc gcaatttgcg ctactgtagc gcggataatc tgactccagg aaactatatg
     4021 cgcaatcgta cgctggctga tcttgatcgt gtcgttgctc tcggcggagg gcatggactg
     4081 ggacgcgttc tctcatcact ttcgtctttg ggttctcgtt taacgggtat cgtcaccacc
     4141 accgataatg gtggctcgac ggggcgtatt cgccgttcag aaggcggcat tgcctggggc
     4201 gatatgcgca actgcctcaa ccagctgata acggaaccga gcgtcgcctc cgcgatgttt
     4261 gaataccgtt ttggtggcaa tggcgaactt tccggtcata atctcggaaa cttgatgtta
     4321 aaggcgctgg atcaccttag cgtgcggcct ctggaagcca tcaatttaat tcgtaatctg
     4381 ctgaaagtgg atacgcattt gattccaatg tcagagcatc ctgttgatct gatggcgatt
     4441 gacgatcagg ggcatgaagt ttacggcgag gtcaatatcg accagttaac tacgccgatt
     4501 caagagttat tgttaacgcc taatgtaccc gcaacgcgtg aggcggttca cgctatcaat
     4561 gaagcggatc tcatcattat tgggcctggc agtttttata ccagcctgat gccaattctg
     4621 ctgctgaagg aaatcgccca ggcattacgc cgcacgccag cgccgatggt ttatatcggc
     4681 aatctggggc gtgagttgag tttacctgcg gctaatttga agctggaaag caagctggca
     4741 attatggagc agtatgttgg taaaaaggtc attgatgcgg tcatcgtcgg gccaaaagtg
     4801 gatgtctcgg cggtgaaaga gcggattgtg atccaggagg tactggaggc cagcgatatt
     4861 ccgtatcgtc atgaccgcca gttgttacat aacgcgctgg aaaaggcgtt acaggcttta
     4921 ggttaacgga aatccgaagg aaaattccgg cttcctattg aagacaaagt gcgcgttgtt
     4981 tatgccggat gcggcgtgaa cgccttatcc ggcctacaaa ccgcgcaaat tcaatatatt
     5041 gcggagaaaa tgtaggcctg ataagcgtag cgcatcaggc tgttttccgt ttgtcatcag
     5101 tcttcttcgc tatcctgtta cgatgccgcg ataaacagct cacgcagctg atgcaactgg
     5161 tcacgaattt gcgccgcttc ttcgaactcc agattctgcg cgtgttgcat catcaaccct
     5221 tccagctcat ggattttctg ctgcaacgct ttaggcgaca tatccatcgg cacattatcc
     5281 ggctcaacaa tcggtcgcga ttttcctctg cccttcgctt tggttttagc aatgttctgc
     5341 cccaacgcca ggatatcgac cactttcttg ttcaagcctt gcggcgtaat gccgtgttcc
     5401 tcgttgtact tctgctgttt ctcgcggcga cgttcggttt cgccaatcgc tttcgccatt
     5461 gatggggtga tcttatcgcc gtagagaatc gctttaccgt taacgttacg tgccgcacga
     5521 ccaatggtct ggatcaacga acgttcggaa cgcaggaagc cttctttgtc agcgtcgagg
     5581 atcgccacca gcgaaacttc cggcatatcc agaccttcac gcagtaagtt gatccctacc
     5641 aatacgtcga actcacccag acgcaagtcg cggataatct ccatacgctc gacggtgtcg
     5701 atatctgagt gaagataacg cacgcgctca ccgtgttctt cgagatattc ggtaagatct
     5761 tccgccattc gcttggtcag tgtggtgacc agtacgcgtt cgttaattgc cgctcgctga
     5821 cgaatctccg aaagaagatc atcaacctgt gtcgccaccg gccgcacttc gataatcggg
     5881 tcaagcaatc cggttggacg caccacctga tccaccacat cgccgccgga tttttccagc
     5941 tcgtaattac ccggcgtcgc cgaaacatag atggtttgcg gcgctaatgc ttcgaactct
     6001 tcaaacttaa gcggacggtt atccagcgct gatggcaggc ggaagccgta ctccaccagt
     6061 gtctctttac gcgcccggtc accgcgatac atgccgccaa tttgtggaat ggtgacgtga
     6121 gattcatcga ccaccagcag cccatcagca gggaggtaat caaacagcgt cggcggtggc
     6181 tcacccggtc cacgaccgga gaggaagcgc gagtagtttt caatccccga acagtagccc
     6241 agctcgttca tcatctccag atcaaactgg gtacgctggg tcagccgctg ctcttccagc
     6301 agtttgttgt tttccaacag cactttgcgt ctggcggcca gctcttcttt gatctcctcc
     6361 atcgcctgta cgatgcgctc gcgcggtgtg acgtagtgcg ttttcgggta gatggtaaaa
     6421 cgtggaatag tggaaacaat ctgcccggtc agcgggtcaa ataacgacaa tcgttccact
     6481 tcctcgtcaa acagttccac gcgaagtgca atgtcatccg attctgccgg gaagatatct
     6541 atcacctcgc cacgaacgcg gaaagtacca cgctggaatg cttgatcatt acgagcgtat
     6601 tgcagctccg ccagtcggcg cagaatcgcg cgctgatcga taatcatacc gaccgtgaga
     6661 tggagcatca tcttgagata taaatcagga tcgcccagac cataaatcgc ggaaacagac
     6721 gccaccacaa ccacatcacg ccgctccagc atcgctttgg tggcggacaa acgcatctgc
     6781 tcaatatgtt cgttaaccga ggcatctttc tcaatgaaag tgtcggaact cggtacatag
     6841 gcttccggct gatagtagtc gtagtaggaa acgaaatatt ccaccgcgtt ttccgggaag
     6901 aactctttca tttcgccata cagctgggcc gccagcgttt tgttgggcgc aagtaccatg
     6961 gttgggcgct gaaggtcagc aatgacattg gcaatggtga atgttttccc ggagccagtc
     7021 acgccaagta acgtctggtg cgccaggcca tcttccagcc cctcttcgag acgtcgaatc
     7081 gcctctggct gatcgccaga aggtttaaaa gcggaattca gtttgaacgg tttactcatg
     7141 agtcgctacc tgaaggagtt gggcgggcag gtatgtaatt ttactcgtcg tacttaatta
     7201 tgccaacaaa ttatactgg
//
LOCUS       DAZKHF020000218         7179 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2201S31241
            SAMN43267800-rid25106413.denovo.220, whole genome shotgun sequence.
ACCESSION   DAZKHF020000218 DAZKHF020000000
VERSION     DAZKHF020000218.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267800
            Sequence Read Archive: SRR30307263
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 7179)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7179)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 7179)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350581.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350581.2
            Genome Coverage       :: 151x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:21:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,652
            CDSs (total)                      :: 5,557
            Genes (coding)                    :: 5,320
            CDSs (with protein)               :: 5,320
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 237
            CDSs (without protein)            :: 237
            Pseudo Genes (ambiguous residues) :: 0 of 237
            Pseudo Genes (frameshifted)       :: 102 of 237
            Pseudo Genes (incomplete)         :: 130 of 237
            Pseudo Genes (internal stop)      :: 52 of 237
            Pseudo Genes (multiple problems)  :: 41 of 237
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7179
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267800-rid25106413.denovo.220"
                     /serotype="O 26:H11"
                     /isolate="2201S31241"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-01"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            complement(60..1652)
                     /gene="ybiT"
                     /locus_tag="ACHOSC_RS18785"
                     /old_locus_tag="pgaptmp_003757"
     CDS             complement(60..1652)
                     /gene="ybiT"
                     /locus_tag="ACHOSC_RS18785"
                     /old_locus_tag="pgaptmp_003757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATPase"
                     /protein_id="HGU4549610.1"
                     /translation="MLVSSNVTMQFGSKPLFENISVKFGGGNRYGLIGANGSGKSTFM
                     KILGGDLEPTLGNVSLDPNERIGKLRQDQFAFEEFTVLDTVIMGHKELWEVKQERDRI
                     YALPEMSEEDGYKVADLEVKYGEMDGYSAEARAGELLLGVGIPVEQHYGPMSEVAPGW
                     KLRVLLAQALFADPDILLLDEPTNNLDIDTIRWLEQVLNERDSTMIIISHDRHFLNMV
                     CTHMADLDYGELRVYPGNYDEYMTAATQARERLLADNAKKKAQIAELQSFVSRFSANA
                     SKSRQATSRARQIDKIKLEEVKASSRQNPFIRFEQDKKLFRNALEVEGLTKGFDNGPL
                     FKNLNLLLEVGEKLAVLGTNGVGKSTLLKTLVGDLQPDSGTVKWSENARIGYYAQDHE
                     YEFENDLTVFEWMSQWKQEGDDEQAVRSILGRLLFSQDDIKKPAKVLSGGEKGRMLFG
                     KLMMQKPNILIMDEPTNHLDMESIESLNMALELYQGTLIFVSHDREFVSSLATRILEI
                     TPERVIDFSGNYEDYLRSKGIE"
     gene            1871..2791
                     /gene="ldtB"
                     /locus_tag="ACHOSC_RS18790"
                     /old_locus_tag="pgaptmp_003758"
     CDS             1871..2791
                     /gene="ldtB"
                     /locus_tag="ACHOSC_RS18790"
                     /old_locus_tag="pgaptmp_003758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase"
                     /protein_id="HGU4549611.1"
                     /translation="MNMKLKTLFAAAFAVVGFCSTASAVTYPLPTDGSRLVGQNQVIT
                     IPEGNTQPLEYFAAEYQMGLSNMMEANPGVDTFLPKGGTVLNIPQQLILPDTVHEGIV
                     INSAEMRLYYYPKGTNTVIVLPIGIGQLGKDTPINWTTKVERKKAGPTWTPTAKMHAE
                     YRAAGEPLPAVVPAGPDNPMGLYALYIGRLYAIHGTNANFGIGLRVSHGCVRLRNEDI
                     KFLFEKVPVGTRVQFIDEPVKATTEPDGSRYIEVHNPLSTTEAQFEGQEIVPITLTKS
                     VQTVTGQPDVDQVVLDEAIKNRSGMPVRLN"
     gene            complement(2850..3968)
                     /gene="ybiR"
                     /locus_tag="ACHOSC_RS18795"
                     /old_locus_tag="pgaptmp_003759"
     CDS             complement(2850..3968)
                     /gene="ybiR"
                     /locus_tag="ACHOSC_RS18795"
                     /old_locus_tag="pgaptmp_003759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anion transporter"
                     /protein_id="HGU4549612.1"
                     /translation="MSLPFLRTLQGDRFFQLLILVGIGLSFFVPFAPKSWPAAIDWHT
                     IITLSGLMLLTKGVELSGYFDVLGRKMVRRFATERRLAMFMVLAAALLSTFLTNDVAL
                     FIVVPLTITLKRLCEIPVNRLIIFEALAVNAGSLLTPIGNPQNILIWGRSGLSFAGFI
                     AQMAPLAGAMMLTLLLLCWCCFPGKALQYHTGVQTPEWKPRLVWSCLGLYIVFLTALE
                     FKQELWGLVIVAAGFALLARRVVLSVDWTLLLVFMAMFIDVHLLTQLPALQGVLGNVS
                     HLSEPGLWLTAIGLSQVISNVPSTILLLNYVPPSLLLVWAVNVGGFGLLPGSLANLIA
                     LRMANDRRIWWRFHLYSIPMLLWAALVGYVLLVILPAN"
     gene            complement(3965..4432)
                     /gene="mntR"
                     /locus_tag="ACHOSC_RS18800"
                     /old_locus_tag="pgaptmp_003760"
     CDS             complement(3965..4432)
                     /gene="mntR"
                     /locus_tag="ACHOSC_RS18800"
                     /old_locus_tag="pgaptmp_003760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0046914 - transition metal ion binding
                     [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="manganese-binding transcriptional regulator
                     MntR"
                     /protein_id="HGU4549613.1"
                     /translation="MSRRAGTPTAKKVTQLVNVEEHVEGFRQVREAHRRELIDDYVEL
                     ISDLIREVGEARQVDMAARLGVSQPTVAKMLKRLATMGLIEMIPWRGVFLTAEGEKLA
                     QESRERHQIVENFLLVLGVSPEIARRDAEGMEHHVSEETLDAFRLFTQKHGAK"
     gene            4618..4746
                     /gene="mntS"
                     /locus_tag="ACHOSC_RS18805"
                     /old_locus_tag="pgaptmp_003761"
     CDS             4618..4746
                     /gene="mntS"
                     /locus_tag="ACHOSC_RS18805"
                     /old_locus_tag="pgaptmp_003761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="manganase accumulation protein MntS"
                     /protein_id="HGU4549614.1"
                     /translation="MNEFKRCMRVFSHSPFKVRLMLLSMLCDMVNNKPQQDKPSDK"
     gene            5018..6601
                     /gene="opgE"
                     /locus_tag="ACHOSC_RS18810"
                     /old_locus_tag="pgaptmp_003762"
     CDS             5018..6601
                     /gene="opgE"
                     /locus_tag="ACHOSC_RS18810"
                     /old_locus_tag="pgaptmp_003762"
                     /EC_number="2.7.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoethanolamine transferase"
                     /protein_id="HGU4549615.1"
                     /translation="MNLTLKESLVTRSRVFSPWTAFYFLQSLLINLGLGYPFSLLYTA
                     AFTAILLLLWRTLPRVQKVLVGISSLVAACYFPFAQAYGAPNFNTLLALHSTNMEEST
                     EILTIFPWYSYMVGIFIFVLGVIAIRRKKESEKARWNTFDSLCLVFSVATFFVAPVQN
                     LAWGGVFKLKDTGYPVFRFAKDVIVNNNEVIEEQERMAKLSGMKDTWTVTAVKPKYQT
                     YVVVIGESARRDALGAFGGHWDNTPFASSVNGLIFADYIAASGSTQKSLGLTLNRVVD
                     GKPQFQDNFVTLANRAGFQTWWFSNQGQIGEYDTAIASIAKRADEVYFLKEGNFEADK
                     NTKDEALLDMTAQVLAQEHSQPQLIVLHLMGSHPQACDRTQGKYETFVQSKETSCYLY
                     TMTQTDDLLRKLYDQLRNSGSSFSLVYFSDHGLAFKERGKDVQYLAHDDKYQQNFQVP
                     FMVISSDDKAHRVIKARRSANDFLGFFSQWTGIKAKEINIKYPFISEKKAGPIYITNF
                     QLQKVDYNHLGTDIFDPKP"
     gene            complement(6650..7165)
                     /gene="ompX"
                     /locus_tag="ACHOSC_RS18815"
                     /old_locus_tag="pgaptmp_003763"
     CDS             complement(6650..7165)
                     /gene="ompX"
                     /locus_tag="ACHOSC_RS18815"
                     /old_locus_tag="pgaptmp_003763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein OmpX"
                     /protein_id="HGU4549616.1"
                     /translation="MKKIACLSALAAVLAFTAGTSVAATSTVTGGYAQSDAQGQMNKM
                     GGFNLKYRYEEDNSPLGVIGSFTYTEKSRTASSGDYNKNQYYGITAGPAYRINDWASI
                     YGVVGVGYGKFQTTEYPTYKHDTSDYGFSYGAGLQFNPMENVALDFSYEQSRIRSVDV
                     GTWIAGVGYRF"
BASE COUNT         1806 a         1942 c         1706 g         1725 t
ORIGIN      
        1 attttgcatt tgtcatcagc cgccgtcacc ccgacagtat cggggtgacg gataatcact
       61 tactcgatcc ctttactacg caggtaatct tcgtaattac cgctaaagtc gatcacgcgt
      121 tccggggtga tttccagaat gcgggtcgcc agggagctta cgaactcacg gtcgtgtgaa
      181 acaaagatca gcgtgccctg atacagttcc agcgccatgt tcagtgactc gatggattcc
      241 atatccaggt ggttggtcgg ttcgtccatg atcagaatgt tcggcttctg catcattaac
      301 ttaccaaaca gcatccgccc tttttcccca ccggaaagca ctttagctgg ctttttgatg
      361 tcgtcctggc tgaacagcaa acgaccgaga atgctgcgta ccgcctgctc gtcatcgcct
      421 tcctgcttcc actgactcat ccattcgaac acggtcagat cattttcaaa ctcatattcg
      481 tggtcctggg cgtagtaacc aatgcgcgcg ttttcagacc attttacggt gccgctgtcc
      541 ggttgcagat cgcccaccag cgttttcagc agcgttgatt taccgacgcc gttggtaccc
      601 agtaccgcaa gtttttcacc gacctccagc agcaggttga gatttttaaa cagtggaccg
      661 ttatcaaacc ctttggtcag accttccact tccagcgcgt tacggaacag tttcttatcc
      721 tgttcaaaac ggatgaacgg gttctgacgg ctggaggctt tcacctcttc cagtttgatt
      781 ttatcaatct ggcgcgcgcg cgaagttgcc tggcgagatt tcgaggcgtt ggcgctaaag
      841 cggctaacga aagattgcaa ctcagcaatc tgcgctttct tcttggcgtt atcggccagc
      901 agacgttcac gcgcctgggt cgccgccgtc atgtactcat cgtagttacc cggataaacg
      961 cgcagctcgc cgtaatccag atccgccatg tgggtacaga ccatgttaag gaagtgacgg
     1021 tcgtgcgaga tgatgatcat ggtgctgtca cgctcgttca gcacctgttc cagccagcga
     1081 atggtgtcga tgtcgaggtt gttggtcggt tcgtcgagca ggagaatatc cggatcagca
     1141 aacagcgcct gcgccagaag cacacgcagc ttccagccag gagcaacttc actcatcggg
     1201 ccgtagtgct gttccactgg aattcccacg ccaagcaaca attcaccggc gcgagcttcc
     1261 gcagagtaac cgtccatttc gccgtattta acttccagat cggccacttt atagccgtct
     1321 tcctcactca tttccggcaa agcatagatg cggtcgcgct cctgcttcac ttcccacaac
     1381 tctttatgcc ccatgatcac cgtatccagc acagtgaact cttcaaaggc aaactgatcc
     1441 tgacgcagct taccaatgcg ctcgttggga tcgagggaaa cgttacccaa agtcggctct
     1501 aagtcgccgc cgaggatctt cataaaggtg gatttaccac taccgttcgc gccaatcagg
     1561 ccgtaacggt tgccgccgcc aaatttgacg gaaatgtttt caaacaacgg cttactgccg
     1621 aactgcatgg tgacgttact ggaaactaac acaggcgtat cctgaaaaga gatatgacaa
     1681 accgcgcatt atgccataag tctgaaagaa gatcctgctc ctcgcttaag gtctacactc
     1741 aagccaaagg caaaaaatgt gatttcgtac acatctgatt tcactgtgag ctggaatgaa
     1801 cttataatgc gcttccaata ctctaatatt ctcaacccaa tggcctgcca ggcacaaaat
     1861 ctcgcttaac atgaatatga aattgaaaac attattcgca gcggccttcg ctgttgtcgg
     1921 cttttgcagt accgcctctg cggtaactta tcctctgcca accgacggga gtcgcctggt
     1981 tggtcagaat caggtgatca ccattcctga aggtaacact cagccgctgg agtattttgc
     2041 cgcggagtac cagatggggc tttccaatat gatggaagcg aacccgggtg tggatacctt
     2101 cctgccgaaa ggcggtactg tactgaacat tccgcagcag ctgatcctgc cggataccgt
     2161 tcatgaaggc atcgtcatta acagtgcaga gatgcgtctg tattactatc cgaaagggac
     2221 caacaccgtt atcgtgctgc cgatcggcat tggtcagtta ggcaaagata cgcctatcaa
     2281 ctggaccacc aaagttgagc gtaagaaagc aggcccgacc tggacgccga ccgccaaaat
     2341 gcacgcagag taccgcgctg cgggcgaacc gcttccggct gtcgttccgg caggtccgga
     2401 taacccgatg ggtctgtatg cactctacat cggtcgcctg tatgctatcc atggcaccaa
     2461 cgccaacttc ggtatcggcc tgcgtgtaag tcatggttgt gtgcgtctgc gtaacgaaga
     2521 catcaaattc ctgttcgaga aagtaccggt cggtacccgc gtacagttta ttgatgagcc
     2581 ggtaaaagcg accaccgagc cagacggcag ccgttatatt gaagtccata acccgctgtc
     2641 taccaccgaa gcccagtttg aaggtcagga aattgtgcca attaccctga cgaagagcgt
     2701 gcaaacagtg accggtcagc cagatgttga ccaggttgtt cttgatgaag cgattaaaaa
     2761 ccgctccggg atgccggttc gtctgaatta atcttcaaaa cttaaagcaa aaggcggact
     2821 cataatccgc cttttttatt tgccagaccc tagttggccg ggagtataac taacaaaaca
     2881 tatcccacca acgccgccca caacagcatc ggtattgaat agagatggaa acgccaccag
     2941 atgcggcgat cgttcgccat acgtagcgca atcaaatttg ccagcgatcc gggtaataac
     3001 ccaaagccac ctacgtttac cgcccatacc agtaataaag acggcggcac atagttcagc
     3061 aacaatatgg tactcggcac gttactgatc acctgcgata aaccgattgc cgttaaccat
     3121 aacccgggtt cagatagatg actcacgtta cccaacacgc cttgcaacgc tggaagctgg
     3181 gtcagtaaat ggacgtcgat aaacatcgcc ataaacacca gcagcagcgt ccagtccaca
     3241 ctgagcacca cgcgacgtgc cagcagcgca aagcctgccg ccacaatcac cagtccccac
     3301 agctcttgtt tgaactccag cgccgtcaga aagacgatat acagccccag acaactccac
     3361 accagccgcg gtttccactc cggtgtttgc acccccgtat ggtattgcag cgcctttcca
     3421 gggaaacaac accagcacag gagcaggagc gtcagcatca ttgcgccagc cagcggtgcc
     3481 atttgggcaa taaatccggc aaacgaaaga ccagaacgtc cccagataag aatattttgc
     3541 gggttgccaa ttggcgtcag tagcgaacca gcgttgactg ccagcgcctc aaaaataatc
     3601 agccgattaa ccgggatctc acacagtctt tttagcgtga tagtcagcgg aacaacaata
     3661 aacagcgcga catcgttggt cagaaaggta gaaagcagcg ccgccgccag caccataaac
     3721 atcgccagcc gacgctccgt agcaaagcgg cgcaccattt tgcgccccag cacatcaaaa
     3781 taaccgctta actccacacc tttggtcagc agcatcaggc cgcttaaggt gatgatggtg
     3841 tgccagtcga tagcagcagg ccaggatttc ggtgcaaagg gcacgaaaaa gcttaatccg
     3901 ataccaacaa gaattaataa ctgaaaaaaa cgatcgcctt gcagcgtgcg taaaaaaggc
     3961 aggctcattt ggcaccgtgt ttctgggtaa acaaacgaaa agcgtcgagc gtctcttcac
     4021 taacatggtg ctccatgcct tccgcgtcgc gacgggcgat ttccggactg acgcccaaca
     4081 ccaacaagaa attttcgact atctgatgtc gctcgcggct ttcctgcgcc agcttctctc
     4141 cttctgccgt taaaaacacg ccacgccagg ggatcatttc aatcagcccc atggttgcca
     4201 gccgcttaag cattttagcc accgtcggtt gcgaaactcc cagacgagca gccatgtcca
     4261 cctgacgagc ttcccccact tccctgatca agtcagaaat cagctcaacg taatcatcaa
     4321 taagctcgcg ccgatgcgcc tctctgacct ggcggaaccc ttcaacgtgc tcttccacgt
     4381 tcactaactg cgtcactttt tttgctgttg gcgtacctgc gcgacgactc attgtgcttc
     4441 ctcattatgg tgacgcatac atagcgttct catttcgtgc gcacattgta aaccagagtt
     4501 gcgaaggtac aaaaaattaa cgttttagca atagctatat aatatagcct gtgctatatc
     4561 tgtatgtaat gcaatcatcc ctcaaggatc gacgggatta gcaagtcagg aggtcttatg
     4621 aatgagttca agaggtgtat gcgcgtgttt agtcattctc cctttaaagt acggttaatg
     4681 ctgctctcta tgttgtgcga tatggtcaac aacaaaccgc agcaagataa accttccgat
     4741 aaatagcggc gtcgcggtac gccgcttcac tcctgctttc atgcaggcat aacgcgtttt
     4801 ggtctgaaaa accccacttt ttgtcggatt tgcaatcccc ttcgcaaaag atttgttcgt
     4861 cagtagttga cctgaacggc ggctcgctct atcttcttgc agccctgcgt atattgcggc
     4921 tcgcggatgc ggaccccttt cccctcttca cgcactcttg caggtattga cccttgacgc
     4981 cagggtaagc acatggcgtt tgttacgata gtggcatatg aatttaaccc tcaaagaatc
     5041 gcttgttacc cgtagccggg tatttagccc gtggactgcg ttctactttt tacagtcgct
     5101 attaattaac ctcggcttag gttacccctt cagtttgctc tacaccgctg cgtttacggc
     5161 tattttgctt ttgctatggc gaacattgcc tcgcgtacaa aaagttctgg tcggtatcag
     5221 ttcgctggtg gcggcttgtt atttcccttt tgctcaggcc tacggcgcgc ctaacttcaa
     5281 tacattgctg gcattgcact ccaccaatat ggaagagtcg accgaaatcc tgacgatttt
     5341 tccgtggtac agctacatgg tcggcatatt tatttttgtg ctcggcgtaa tagcaatcag
     5401 gcgaaaaaaa gagagtgaaa aagcgcgctg gaataccttc gacagcctgt gtctggtatt
     5461 cagtgtggcg acattttttg ttgctcccgt gcaaaacctg gcctggggtg gcgtatttaa
     5521 actgaaagat actggctatc cggtatttcg ttttgctaag gatgtcatcg tcaataataa
     5581 cgaggtgatt gaagagcaag aacggatggc aaaactttcc ggaatgaaag atacctggac
     5641 ggtcactgcc gttaagccga agtatcagac ctatgtggtg gtgatcggtg aaagcgcgcg
     5701 tcgcgatgcc ctcggtgcct ttggcggtca ctgggacaat accccgtttg ccagcagcgt
     5761 taacggtttg atatttgctg actacattgc cgccagtggc tccacgcaga aatcgcttgg
     5821 cttaacgctc aatcgcgtag tcgatggcaa accacagttt caggataact ttgtcaccct
     5881 ggcaaatcgc gcgggcttcc agacctggtg gttttccaac cagggccaaa tcggcgaata
     5941 cgatactgct atcgccagca tcgccaaacg tgcagatgaa gtgtacttcc tgaaagaagg
     6001 taattttgaa gcagataaaa acacgaaaga cgaagcgtta ctggatatga ccgctcaagt
     6061 gctggcgcaa gagcactcgc aaccgcagct gattgttctg catctgatgg gctcgcatcc
     6121 gcaggcctgc gacaggacac aaggcaaata cgaaaccttt gtgcaatcga aagaaacgtc
     6181 gtgctatctc tataccatga cgcaaacgga cgatttactg cgcaagctgt acgatcagtt
     6241 acgcaacagc ggcagcagct tctctctggt ttacttttct gaccacggtc tggcctttaa
     6301 agagcgcggc aaagacgtgc aataccttgc ccatgatgat aaatatcagc aaaatttcca
     6361 ggtgcctttt atggtcattt ccagcgacga taaagcgcat cgggtgatta aagcccgccg
     6421 ctcagccaat gacttcttag gctttttctc gcagtggacg ggaattaaag cgaaggaaat
     6481 aaatatcaaa tacccgttta tatctgagaa gaaagccggg ccgatataca tcaccaactt
     6541 ccagttacag aaggtggatt acaaccatct cggaaccgat attttcgacc cgaaacctta
     6601 aaacaaaaaa tccgccccga gaggcggatt ttttatatca ccaaagtgat tagaagcggt
     6661 aaccaacacc ggcaatccag gtacctacgt caacgctacg aatacggctc tgctcgtaag
     6721 agaagtccag agcaacgttt tccatcgggt tgaactgcag accagcaccg taggagaaac
     6781 cgtagtcgct ggtgtcgtgt ttgtaggtcg ggtattccgt ggtctggaat ttaccataac
     6841 ccacccccac tacaccgtag atgcttgccc agtcgttaat gcggtaagcc ggaccagcag
     6901 tgatgccgta gtactggttt ttgttgtagt caccagagct tgcagtacgg cttttctcgg
     6961 tgtaagtgaa agagccgatc acacccagcg ggctgttgtc ttcttcatag cggtatttca
     7021 ggttgaaacc gcccattttg ttcatttggc cctgagcgtc gctctgtgcg taaccgccag
     7081 ttacggtaga agtcgcagct acggaagtac ctgcggtgaa agccagaact gcggccagtg
     7141 ctgaaagaca tgcaattttt ttcataacca cctcaaatg
//
LOCUS       DAZKHF020000219         7169 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2201S31241
            SAMN43267800-rid25106413.denovo.221, whole genome shotgun sequence.
ACCESSION   DAZKHF020000219 DAZKHF020000000
VERSION     DAZKHF020000219.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267800
            Sequence Read Archive: SRR30307263
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 7169)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7169)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 7169)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350581.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350581.2
            Genome Coverage       :: 151x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:21:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,652
            CDSs (total)                      :: 5,557
            Genes (coding)                    :: 5,320
            CDSs (with protein)               :: 5,320
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 237
            CDSs (without protein)            :: 237
            Pseudo Genes (ambiguous residues) :: 0 of 237
            Pseudo Genes (frameshifted)       :: 102 of 237
            Pseudo Genes (incomplete)         :: 130 of 237
            Pseudo Genes (internal stop)      :: 52 of 237
            Pseudo Genes (multiple problems)  :: 41 of 237
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7169
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267800-rid25106413.denovo.221"
                     /serotype="O 26:H11"
                     /isolate="2201S31241"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-01"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            60..1109
                     /gene="allD"
                     /locus_tag="ACHOSC_RS18820"
                     /old_locus_tag="pgaptmp_003764"
     CDS             60..1109
                     /gene="allD"
                     /locus_tag="ACHOSC_RS18820"
                     /old_locus_tag="pgaptmp_003764"
                     /EC_number="1.1.1.154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ureidoglycolate dehydrogenase"
                     /protein_id="HGU4549617.1"
                     /translation="MKISRETLHQLIENKLCQAGLKREHAATVAEVLVYADARGIHSH
                     GAVRVEYYAERISKGGTNREPEFRLEETGPCSAILHADNAAGQVAAKMGMEHAIKTAQ
                     QNGVAVVGISRMGHSGAISYFVQQAARAGLIGISMCQSDPMVVPFGGAEIYYGTNPLA
                     FAAPGEGDEILTFDMATTVQAWGKVLDARSRNMSIPDTWAVDKNGAPTTDPFAVHALL
                     PAAGPKGYGLMMMIDVLSGVLLGLPFGRQVSSMYDDLHAGRNLGQLHIVINPNFFFSS
                     KLFRQHLSQTMRELNAITPAPGFNQVYYPGQDQDIKQRKAAVEGIEIVDDIYQYLISD
                     ALYNTSYETKNPFAQ"
     gene            1131..2366
                     /gene="allC"
                     /locus_tag="ACHOSC_RS18825"
                     /old_locus_tag="pgaptmp_003765"
     CDS             1131..2366
                     /gene="allC"
                     /locus_tag="ACHOSC_RS18825"
                     /old_locus_tag="pgaptmp_003765"
                     /EC_number="3.5.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047652 - allantoate deiminase activity
                     [Evidence IEA];
                     GO_process: GO:0000256 - allantoin catabolic process
                     [Evidence IEA];
                     GO_process: GO:0009442 - allantoin assimilation pathway
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="allantoate deiminase"
                     /protein_id="HGU4549618.1"
                     /translation="MITHFRQAIEETLPWLSSFGADPTGGMTRLLYSPEWLETQQQFK
                     KRMAASGLETRFDEVGNLYGRLSGTEYPQEVVLSGSHIDTVVNGGNLDGQFGALAAWL
                     AIDWLKTQYGAPLRTVEVVAMAEEEGSRFPYVFWGSKNIFGLANPDDVRNICDAKGNS
                     FVDAMKACGFTLPNAPLTPRQDIKAFVELHIEQGCVLESNGQSIGVVNAIVGQRRYTV
                     TLNGESNHAGTTPMGYRRDTVYAFSRICHQSVEKAKRMGDPLVLTFGKVEPRPNTVNV
                     VPGKTTFTIDCRHTDAAVLRDFTQQLENDMRAICDEMDIGIDIDLWMDEEPVPMNKEL
                     VATLTELCEREKLNYRVMHSGAGHDAQIFAPRVPTCMIFIPSINGISHNPAERTNITD
                     LAEGVKTLALMLYQLAWQK"
     gene            2377..3162
                     /gene="allE"
                     /locus_tag="ACHOSC_RS18830"
                     /old_locus_tag="pgaptmp_003766"
     CDS             2377..3162
                     /gene="allE"
                     /locus_tag="ACHOSC_RS18830"
                     /old_locus_tag="pgaptmp_003766"
                     /EC_number="3.5.3.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0071522 - ureidoglycine aminohydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0010136 - ureide catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(S)-ureidoglycine aminohydrolase"
                     /protein_id="HGU4549619.1"
                     /translation="MGYLNNVTGYRDDLLANRAIVKHGNFALLTPDGLVKNIIPGFEN
                     CDATILSTPKLGASFVDYLVTLHQNGGNQQGFGGEGIETFLYVISGNITAKAEGKTFA
                     LSEGGYLYCPPGSLMTFVNAQAEDSQIFLYKRRYVPVEGHAPWLVSGNASELERIHYE
                     GMDDVILLDFLPKELGFDMNMHILSFAPGASHGYIETHVQEHGAYILSGQGVYNLDNN
                     WIPVKKGDYIFMGAYSLQAGYGVGRGEAFSYIYSKDCNRDVEI"
     gene            complement(3291..4436)
                     /gene="glxK"
                     /locus_tag="ACHOSC_RS18835"
                     /old_locus_tag="pgaptmp_003767"
     CDS             complement(3291..4436)
                     /gene="glxK"
                     /locus_tag="ACHOSC_RS18835"
                     /old_locus_tag="pgaptmp_003767"
                     /EC_number="2.7.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerate 3-kinase"
                     /protein_id="HGU4549620.1"
                     /translation="MKIVIAPDSFKESLSAEKCCQAIKAGFSTLFPDANYICLPIADG
                     GEGTVDAMVAATGGNIVTLEVCGPMGETVNAFYGLTGDGKTAVIEMAAASGLMLVAPE
                     KRNPLLASSFGTGELIRHALDNGIRHIILGIGGSATVDGGMGMAQALGVRFLDADGQV
                     LAANGGNLARVASIEMDECDPRLANCHIEVACDVDNPLVGARGAAAVFGPQKGATPEM
                     VEELEQGLQNYARVLQQQTEINVCQMAGGGAAGGMGIAAAVFLNADIKPGIEIVLRAV
                     NLEQAVQGAALVITGEGRIDSQTAGGKAPLGVASVAKQFNVPVIGIAGVLGDGVEVVH
                     QYGIDAVFSILPRLAPLAEVLASGETNLFNSARNIACAIKIGQGIKN"
     gene            complement(4458..5758)
                     /locus_tag="ACHOSC_RS18840"
                     /old_locus_tag="pgaptmp_003768"
                     /pseudo
     CDS             complement(4458..5758)
                     /locus_tag="ACHOSC_RS18840"
                     /old_locus_tag="pgaptmp_003768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308602.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="uracil/xanthine transporter"
     gene            complement(5815..>7169)
                     /gene="allB"
                     /locus_tag="ACHOSC_RS18845"
                     /old_locus_tag="pgaptmp_003769"
     CDS             complement(5815..>7169)
                     /gene="allB"
                     /locus_tag="ACHOSC_RS18845"
                     /old_locus_tag="pgaptmp_003769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004038 - allantoinase activity [Evidence
                     IEA];
                     GO_process: GO:0000256 - allantoin catabolic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="allantoinase AllB"
                     /protein_id="HGU4549621.1"
                     /translation="DLIIKNGTVILENEARVVDIAVKGGKIAAIGQDLGDAKEVMDAS
                     GLVVSPGMVDAHTHISEPGRSHWEGYETGTRAAAKGGITTMIEMPLNQLPATVDRASI
                     ELKFDAAKGKLTIDAAQLGGLVSYNIDRLHELDEVGVVGFKCFVATCGDRGIDNDFRD
                     VNDWQFFKGAQKLGELGQPVLVHCENALICDALGEEAKREGRVTAHDYVASRPVFTEV
                     EAIRRVLYLAKVAGCRLHVCHISSPEGVEEVTRARQEGQDVTCESCPHYFVLDTDQFE
                     EIGTLAKCSPPIRDLENQKGMWEKLFNGEIDCLVSDHSPCPPEMKAGNIMEAWGGIAG
                     LQNCMDVMFDEAVQKRGMSLPMFGKLMATNAADIFGLQQKGRIAPGKDADFVFIQPNS
                     SYVLTNDDLEYRHKVSPYVGRTIGARITKTILRGDVIYDIEQGFPVAPKGQFILKHQQ
                     "
BASE COUNT         1847 a         1907 c         1782 g         1633 t
ORIGIN      
        1 tattttccct gtatgttaat tgcagtcatg cttcacaccg tcgttaaaaa ggaagacaga
       61 tgaaaatcag tcgggaaaca ctccaccagc taattgagaa taaactctgc caggctgggt
      121 taaaacgtga gcacgctgca accgtggctg aagtattggt ttacgccgat gccagaggga
      181 tccactctca tggcgcggtg cgcgtggaat actacgcgga acgcatttca aaaggcggca
      241 ccaaccgcga accggagttt cgtcttgagg aaaccgggcc gtgctcggca attttacatg
      301 ccgacaatgc cgccggacag gtcgcggcga aaatgggtat ggaacatgcc atcaaaaccg
      361 cccagcaaaa tggcgttgcg gtggtcggta tcagccggat gggtcacagc ggcgcaatct
      421 cttattttgt gcagcaggca gcccgcgccg gattaattgg tatttcgatg tgccagtccg
      481 atccaatggt ggtgccgttt ggcggcgcgg aaatttacta cggtactaac ccactggcct
      541 ttgccgcgcc gggagaaggc gacgagatcc ttacctttga tatggcgact accgtacagg
      601 catggggaaa agtgctcgac gcccgctcgc gtaatatgtc tatcccggat acctgggcgg
      661 tcgataaaaa cggtgcacca acaaccgatc cgttcgcggt acatgctctg ctccccgccg
      721 ccgggccaaa agggtatggc ctgatgatga tgattgacgt cctctcaggc gtcttactcg
      781 gcttaccgtt cgggcgacag gttagttcga tgtatgacga tttacacgcc gggcgtaatt
      841 tggggcaatt acatatcgtt attaacccga actttttctt ctccagcaaa ttatttcgtc
      901 aacatcttag ccagaccatg cgcgaattaa atgccattac ccccgcgccc ggttttaatc
      961 aggtttatta tcccggacag gatcaggata ttaaacaacg caaagccgcc gtcgaaggca
     1021 tcgaaattgt tgatgatatt taccagtatt taatttccga cgcgctttat aacacgtcat
     1081 acgaaacgaa aaatcccttt gcgcaataat tattgagaca ggactttctt atgattacac
     1141 attttcgtca agccatagaa gaaacgctgc cctggctttc ctcttttggc gctgacccaa
     1201 cgggtggtat gacccgttta ctttattcgc cggaatggct ggaaactcag cagcaattta
     1261 aaaaaagaat ggcagcaagc gggctggaaa cacgtttcga tgaagtgggg aatttatatg
     1321 gtcgcctgag tggcaccgaa tatccacaag aagtggttct gagcggttcg catatcgata
     1381 ccgtggttaa cggcggtaac ctcgacgggc aattcggcgc gctggcggcg tggctggcaa
     1441 ttgactggct gaaaacgcaa tacggcgcgc cgctacgtac ggtcgaagtg gtggcgatgg
     1501 cagaagaaga aggcagccgc ttcccgtatg tcttctgggg cagtaaaaat atctttgggc
     1561 tggcgaatcc tgacgacgtg cggaatatct gtgatgccaa aggaaatagt tttgtcgatg
     1621 cgatgaaggc ttgcggattt actcttccga acgccccact aactccgcgt caggatatta
     1681 aagcctttgt tgaactgcat attgaacagg gctgtgtgct ggaaagtaat gggcaatcaa
     1741 ttggcgtggt gaatgcaatt gtcgggcagc gtcgttatac ggtaacgctg aacggcgaat
     1801 caaaccatgc aggcaccacg ccgatgggtt atcgtcgtga tacagtttac gctttcagtc
     1861 gcatttgcca tcagtcggtc gaaaaagcga aaaggatggg cgatccgctg gttctgacct
     1921 ttggcaaagt agagccgcgc ccgaatacgg taaatgtggt gccgggtaaa accacgttca
     1981 ccattgattg tcgtcatacc gacgctgccg tgctgcgcga tttcacccaa cagttagaaa
     2041 acgacatgcg ggcgatttgc gatgaaatgg acattggtat tgatatcgat ttatggatgg
     2101 acgaagaacc cgtgccgatg aataaggagc tggtcgccac cctgacagaa ttgtgtgaaa
     2161 gagaaaaact gaattaccgg gtgatgcaca gtggtgccgg gcacgacgcg caaattttcg
     2221 cgcctcgcgt accaacctgc atgattttca ttcccagcat caacgggatc agccataacc
     2281 cggcggaacg caccaatatt accgaccttg ccgaaggggt caaaacgttg gcactcatgc
     2341 tttatcaact tgcctggcag aaataaggag tcataaatgg gatatttaaa taacgtcacc
     2401 ggttaccgcg atgatttact ggctaaccgt gcgattgtta aacacggtaa tttcgcactg
     2461 ttaaccccgg atggtctggt aaaaaatatt attccgggct ttgaaaattg tgacgcgaca
     2521 atcctctcca cgccaaagct gggtgcctct tttgttgatt atctggtcac actgcatcaa
     2581 aacggtggca accaacaggg cttcggtggc gaaggcattg aaacgttcct gtatgtgatc
     2641 tctggaaata tcactgccaa agccgaaggc aaaacatttg ccttaagcga aggtggctat
     2701 ctttattgcc caccaggctc cttaatgacg tttgttaacg cccaggccga agacagccaa
     2761 atctttttat ataagcgccg ctatgttccg gtagaaggcc atgcaccgtg gctggtttct
     2821 ggtaatgcca gcgaactgga acgcattcac tatgaaggta tggatgatgt gatcctgctg
     2881 gatttcctgc ccaaagagtt aggttttgat atgaacatgc atatcctctc ttttgcgcca
     2941 ggtgccagcc acggttatat cgaaacacac gttcaggaac atggtgccta tattctttcc
     3001 ggtcaggggg tttataacct cgacaataac tggatcccgg tgaaaaaagg cgattacatc
     3061 tttatgggcg cttattcttt acaggctggt tatggcgtag ggcgtggtga agcgttcagc
     3121 tatatttact cgaaagattg caaccgcgac gtagagattt aagtcatacc actcgcctga
     3181 ttatttttag ctatgttgga ttttgccacg gtttataccg tggctttttt tgtagtaaat
     3241 tcaggtaaaa aaagatgcga aataaatcgc atccgcttta aaggtaaggg tcagttttta
     3301 attccctgac ctattttaat ggcgcaggca atatttcgcg cgctgttgaa gagattggtt
     3361 tcaccgctgg cgagcacttc ggctaaaggt gccagacgag gcaaaatgct gaataccgcg
     3421 tcaatgccgt actggtgcac cacttccacg ccatcaccca atacgccagc aatcccaatc
     3481 accgggacat taaactgctt cgccaccgac gcaacgccca gcggcgcttt accgcctgcc
     3541 gtttgcgagt caatgcgccc ttccccggtg atcaccagtg ctgcgccctg caccgcctgc
     3601 tccaggttga ccgcccgcag tacaatctca atacccggtt taatatccgc attgaggaat
     3661 accgccgccg caatacccat accgcccgca gcgccgccgc ccgccatctg gcagacatta
     3721 atttcagttt gctgttgtaa aacacgggcg taattttgca gcccctgttc aagttcttcg
     3781 accatctccg gcgttgcccc tttttgcggg ccaaacaccg ccgccgcgcc gcgtgcccct
     3841 accagcgggt tatcaacgtc acatgctact tcaatatggc aattcgccag acgcggatcg
     3901 cattcatcca tctcaatgct tgccacgcgc gctaaattac cgccgttagc cgccagcacc
     3961 tggccgtcgg catcaaggaa acgcacacca agcgcctgag ccatgcccat accgccgtcg
     4021 accgtcgcac tgccgccaat gccgagaata atatggcgaa tgccgttatc cagcgcatga
     4081 cgaattaact cccccgtacc aaaactggag gccagcaacg gattacgctt ttcaggcgcg
     4141 accagcatca ggccacttgc tgccgccatc tcaatcaccg ccgttttccc gtcgccggta
     4201 aggccataaa aagcattcac tgtttcgccc atcggcccgc agacttcaag cgtcacgatg
     4261 ttgccgcccg tcgcggcgac catcgcatcc accgtccctt cgccgccatc cgctatcggc
     4321 aaacagatat agttcgcatc gggaaagagg gtcgaaaacc cggctttaat tgcctgacaa
     4381 catttttctg cacttaagct ctctttaaaa gagtctggcg caatgacaat cttcattatc
     4441 aatccttttt caacaaatta ttcgatgcgt tcccacggaa taagattatc cataagaaca
     4501 gccagtaaaa ttccgaccag taagccgtta ctgagcagag gacgcagcgt taatggaagg
     4561 tcttgcagat acacaggcgg taatgccatt aaaaatatgc cgacaaataa cggcaatgcg
     4621 agtcgataaa tattgcgagc ggtaaaggtt atttgctggc taaacaccag cgcggaaaag
     4681 agtaaaggca gataagaaac cagcatgacc gcgctactca cgggtaaagg gatactgcaa
     4741 aacagtcgtg tgagtgccgg aaccagcgcc accagcaggc aaataacgct gccataaata
     4801 aacgaacgcc gcgtgtaatc gcctgtctgg gttaataaac caatggatga aacaaacggt
     4861 gaaaatggaa ttaccgccag cggtacggtt atcagcgtca taaatccggt cgccacaaag
     4921 ctgcgacgat aacgcgtatt cccgcgccct gctgcggata aaaaacatcc gtgccccgaa
     4981 tcgcaccgta ggtattgctg atattcacca gacctgtaat caccgccgtc agaataattc
     5041 ccggagacaa agcacctcca ctgccgagcg ggaaccactg ccagtgcaac tcaccggaga
     5101 gcgagtgcga agaaggaaag caaaagtacc acaacaacca gccggttatg gtgccgacca
     5161 gcaggccata acgggcaaaa cgttgcggca ggaagataat catccccagt accaggcaca
     5221 tcaccgccac cgagagcgcg aacggcggta actgaatttt aaaatttggg tcggctatgc
     5281 caaacggcag cccgagcata cctttgaaaa agatagtggt cagctgcgcg cccagcatca
     5341 acataaacaa gaccatcacc gacggcgtaa ataaccgtgc caggcgatgg cctaatccgc
     5401 taaaaccaat caacatcgtc agcacgccgg agagcgcaat ccccaccgcc aggctggtag
     5461 cgatatcgtt gatcggtgtc ccgcgcgatg cttcgccaag ggtgatagta aggatggttc
     5521 cccaccacag gccacccggc tcttccataa tagcgcgacg atgaccgcag aacgcctgag
     5581 cgaagcaggc cagtgcggta gcaagaaaag catattgcgt gagcgtaagc aggctacttt
     5641 gcggcaactg aaaagcagaa agtagcgtag gaggaaccac aaccgtgttg caaaaaataa
     5701 agaaaaacca ctgaaatcct gataacaggc tttcgcggct cactgcaaaa ttgaacataa
     5761 acacacctta accggagaat gcccgccgca aggacgggca ttgcaggggc cagattactg
     5821 ctgatgttta aggataaatt gacctttcgg cgcaacaggg aagccctgtt caatgtcgta
     5881 aatcacatca ccacgtaaga tggttttcgt gatacgcgcg ccaatggtac ggccaacata
     5941 cgggctgact ttgtggcgat attccagatc gtcattggta agaacatagc tgctattcgg
     6001 ctgaatgaag acgaagtcgg catcttttcc tggggcgata cggccttttt gctgcagacc
     6061 gaaaatatct gctgcgttag tcgccattaa tttgccgaac attggcagag acattccgcg
     6121 tttctgtacc gcttcatcga acatcacgtc catacagttt tgcagaccgg caataccgcc
     6181 ccatgcttcc atgatgttgc cggctttcat ttccggaggg catggtgagt ggtcggaaac
     6241 caggcagtcg atttcaccgt taaacagttt ttcccacatg cctttctggt tttccagatc
     6301 gcggatcggc ggtgaacact tcgccagagt gccgatttct tcgaactgat cggtatccag
     6361 tacaaagtaa tgcgggcagg attcacaagt aacgtcctga ccttcctgac gtgcacgagt
     6421 cacttcttca acgccttccg ggctgctgat atggcaaacg tgcagacggc aaccggcaac
     6481 tttcgccaga tacagcacgc ggcgaatcgc ttccacttcg gtaaataccg gacgcgaagc
     6541 cacatagtca tgggcagtta cgcgaccttc acgtttcgct tcttcgccca gtgcgtcaca
     6601 gatcagcgcg ttttcgcagt gcaccagcac cggctgcccc agttcgccca gcttctgcgc
     6661 acctttgaag aactgccagt cgtttacatc acggaagtcg ttgtcgatac cgcgatcgcc
     6721 acaggtcgca acgaagcatt tgaagccgac aacgcccact tcatccagct catgcagacg
     6781 gtcgatgttg taagacacca ggccaccgag ttgtgccgca tcgatagtca gcttgccttt
     6841 agcggcatcg aacttcagtt caattgaagc gcggtcaacc gttgcaggca gctggttgag
     6901 cggcatttcg atcatggtgg tgataccacc ttttgccgct gcgcgagtac cggtttcata
     6961 accttcccag tggctacgac ccggttcaga aatatgggtg tgcgcatcaa ccatgcccgg
     7021 cgaaaccacc agaccagacg catccataac ttcttttgca tcgcccagat cctgaccgat
     7081 agcagcaatt ttcccgcctt taacggcgat atctacaacg cgagcttcgt tttctaaaat
     7141 aacggtgccg tttttaatga ttaaatcaa
//
LOCUS       DAZKHF020000220         7047 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2201S31241
            SAMN43267800-rid25106413.denovo.222, whole genome shotgun sequence.
ACCESSION   DAZKHF020000220 DAZKHF020000000
VERSION     DAZKHF020000220.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267800
            Sequence Read Archive: SRR30307263
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 7047)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7047)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 7047)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350581.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350581.2
            Genome Coverage       :: 151x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:21:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,652
            CDSs (total)                      :: 5,557
            Genes (coding)                    :: 5,320
            CDSs (with protein)               :: 5,320
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 237
            CDSs (without protein)            :: 237
            Pseudo Genes (ambiguous residues) :: 0 of 237
            Pseudo Genes (frameshifted)       :: 102 of 237
            Pseudo Genes (incomplete)         :: 130 of 237
            Pseudo Genes (internal stop)      :: 52 of 237
            Pseudo Genes (multiple problems)  :: 41 of 237
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7047
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267800-rid25106413.denovo.222"
                     /serotype="O 26:H11"
                     /isolate="2201S31241"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-01"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            <1..1164
                     /gene="iha"
                     /locus_tag="ACHOSC_RS18850"
                     /old_locus_tag="pgaptmp_003770"
     CDS             <1..1164
                     /gene="iha"
                     /locus_tag="ACHOSC_RS18850"
                     /old_locus_tag="pgaptmp_003770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional siderophore receptor/adhesin Iha"
                     /protein_id="HGU4549622.1"
                     /translation="ENKGRELVRSVLKRDKWGLAGQPRELKESNLILNSLLLTPLGES
                     HLVTVGGEFQSSSMKDGVVLASTGETFRQKSWSVFAEDEWHLTDALALTAGSRYEHHE
                     QFGGHFSPRAYLVWDVADAWTLKGGVTTGYKAPRMGQLHKGISGVSGQGKTNLLGNPD
                     LKPEESVSYEAGVYYDNPAGLNANVTGFMTDFSNKIVSYSINDNTNSYVNSGKARLHG
                     VEFAGTLPLWSEDVTLSLNYTWTRSEQRDGDNKGAPLSYTPEHMVNAKLNWQITEEVA
                     SWLGARYRGKTPRFTQNYSSLSAVQKKVYDEKGEYLKAWTVVDAGLSWKMTDALTLNA
                     AVNNLLNKDYSDVSLYSAGKSTLYAGDYFQTGSSTTGYVIPERNYWMSLNYQF"
     gene            complement(1382..1532)
                     /locus_tag="ACHOSC_RS18855"
                     /old_locus_tag="pgaptmp_003771"
                     /pseudo
     CDS             complement(1382..1532)
                     /locus_tag="ACHOSC_RS18855"
                     /old_locus_tag="pgaptmp_003771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001066419.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase domain-containing protein"
     gene            complement(1744..2064)
                     /locus_tag="ACHOSC_RS18860"
                     /old_locus_tag="pgaptmp_003772"
                     /pseudo
     CDS             complement(1744..2064)
                     /locus_tag="ACHOSC_RS18860"
                     /old_locus_tag="pgaptmp_003772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001053346.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="TerD family protein"
     gene            complement(2487..3062)
                     /locus_tag="ACHOSC_RS18865"
                     /old_locus_tag="pgaptmp_003773"
     CDS             complement(2487..3062)
                     /locus_tag="ACHOSC_RS18865"
                     /old_locus_tag="pgaptmp_003773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006950 - response to stress [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TerD family protein"
                     /protein_id="HGU4549623.1"
                     /translation="MAVSLVKGGNVSLTKEAPTMNVAMVGLGWDARVTDGQGFDLDAS
                     VFAVGEDGKVLSDAHFIFFNNKTSPDGAVEHQGDNRTGEGDGDDEQVKIDLTKVSADI
                     KKLVFAVTIYDAEARKQNFGMVSNSFMRVYNNDNGTEIARFDLSEDASTETAMVFGEL
                     YRHGTEWKFKAVGQGFAGGLSALASQHGVNV"
     gene            complement(3131..3709)
                     /gene="terD"
                     /locus_tag="ACHOSC_RS18870"
                     /old_locus_tag="pgaptmp_003774"
     CDS             complement(3131..3709)
                     /gene="terD"
                     /locus_tag="ACHOSC_RS18870"
                     /old_locus_tag="pgaptmp_003774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tellurium resistance membrane protein TerD"
                     /protein_id="HGU4549624.1"
                     /translation="MSVSLSKGGNVSLSKAAPSMKNVLVGLGWDARSTDGQDFDLDAS
                     AFLLASNGKVRGDSDFIFYNNLTSSDGSVTHTGDNRTGEGDGDDESLKIKLDAVPSEV
                     DKIIFVVTIHDAQARRQSFGQVSGAFIRLVNDDNQTEVARYDLTEDASTETAMLFGEL
                     YRHNGEWKFRAVGQGYAGGLASVCAQYGINAS"
     gene            complement(3758..4798)
                     /gene="terC"
                     /locus_tag="ACHOSC_RS18875"
                     /old_locus_tag="pgaptmp_003775"
     CDS             complement(3758..4798)
                     /gene="terC"
                     /locus_tag="ACHOSC_RS18875"
                     /old_locus_tag="pgaptmp_003775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tellurium resistance membrane protein TerC"
                     /protein_id="HGU4549625.1"
                     /translation="MVSTHIGFPTETVIVFIALSVGAIFIDLFMHRDDKPISLKSAAL
                     WSVFWVVVAMAFAGFLYIHHGAEVASLFVTGYALEKVLSVDNLFVMMAIFSWFAVPDR
                     YRHRVLYWGIIGAIVFRGIFVAIGTSLLSLGPYVEVVFAIIVAWTAVMMLKSGDDDDE
                     IEDYSQHLAYRMVKRFFPIWPKLRGHAFLLNQKEVDAELAKPENSDVTIGRGKKAALY
                     ATPLFLCVAVVELSDVMFAFDSVPAIIAVSREPLIVYSAMMFAILGLRTLYFVLEALK
                     QYLVHLEKAVIVLLFFIAAKLGLNATDHIWHHGYSIAATTSLYVVLGVLALGILASVM
                     FPGKPESEEKGS"
     gene            complement(4821..5276)
                     /locus_tag="ACHOSC_RS18880"
                     /old_locus_tag="pgaptmp_003776"
     CDS             complement(4821..5276)
                     /locus_tag="ACHOSC_RS18880"
                     /old_locus_tag="pgaptmp_003776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tellurite resistance TerB family protein"
                     /protein_id="HGU4549626.1"
                     /translation="MSFFDKVKGAINSGRDELTRQVGRFKNKKFMQGTVAVCARIAVS
                     SDGVSSEEKQKMMGFLRSSEELKVFDTNEVIEFFNKLVSSFDFDVEIGKGETMKYILA
                     LKDQPEAAQLALRVGIAVAKSDGNFDQDEKLASREIAIALGFDPAEFGL"
     gene            complement(5299..6456)
                     /locus_tag="ACHOSC_RS18885"
                     /old_locus_tag="pgaptmp_003777"
     CDS             complement(5299..6456)
                     /locus_tag="ACHOSC_RS18885"
                     /old_locus_tag="pgaptmp_003777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309379.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TerD family protein"
                     /protein_id="HGU4549627.1"
                     /translation="MNLTPGGNAPVPAQELRVRITSGGQVDASAFRLYADGKVQGDAD
                     MVFYGQPRNDDGTVSLVSEGQYSTFTVALNRLKPDVQKIAFTVTCDGGQTVSGLRNLS
                     IDVEQGATGLVSGSVELSGRQEAALILGEFYRRNNDWKFRFVAQGFNGGLKPLAEHFG
                     VNIADEPAPAAPTPPVVTPPPVETKISLSKVSLTKEKPAISLTKRDNFGEIRINLNWH
                     RGSSKSGFAGMFGSKGIDLDLGAFVELQDGYKSVIQALGNAFGDYRDEPYVQLKGDDR
                     TGDVSDGEWLHINGREWKHIREVLIYAFIYEGVPSWDKTDGVVTIHVPDQPPIETRLT
                     EGENRRTLCAIARLVNENGAIKVERINQYFKGQDEMDRAFGWGFRWSAGSK"
     gene            complement(6456..7037)
                     /locus_tag="ACHOSC_RS18890"
                     /old_locus_tag="pgaptmp_003778"
     CDS             complement(6456..7037)
                     /locus_tag="ACHOSC_RS18890"
                     /old_locus_tag="pgaptmp_003778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TerD family protein"
                     /protein_id="HGU4549628.1"
                     /translation="MVSLVKNQTVSLSKESSALSQLHFGLGWDPVKKKGLLGGLFGGN
                     DSIDLDAGCVLMDSTGKTIDTIWFRKLKSTCGAVVHSGDNLTGEGDGDDEVIKVNLSR
                     LPANVEYLAFTVNSFRGQSFNDVENAFCRVVDQTGKELARYKLTEQGSHTGIVISSLR
                     RNNGNWDFTALGHACRGRTIDDMHSDIVSAVIR"
BASE COUNT         1783 a         1828 c         1743 g         1693 t
ORIGIN      
        1 gaaaataaag gtcgtgagct tgtacgcagt gtactgaagc gcgacaaatg ggggcttgcc
       61 ggtcagccgc gggagcttaa ggaatcgaac cttatcctga attcattact gcttacccct
      121 ctgggagaat ctcatctggt tacggtgggg ggcgagtttc agagctcgtc catgaaagac
      181 ggagttgtcc ttgccagcac aggtgaaact ttccggcaga aaagctggtc ggtatttgct
      241 gaggatgagt ggcatctcac ggatgcactt gcgctgactg cgggcagccg ctatgaacat
      301 catgagcaat tcgggggaca cttcagtccg cgtgcatatc tggtctggga tgtggcagat
      361 gcctggacgc tgaaaggcgg tgtgaccacg ggatataagg cacccagaat ggggcagcta
      421 cataaaggga ttagtggtgt gtccgggcag ggaaaaacaa atctacttgg taaccccgac
      481 ctgaagccgg aagagagcgt cagttatgag gctggggtgt attacgataa ccccgccggt
      541 ctgaatgcca atgtcacagg ttttatgact gacttctcca acaagattgt ctcttattcc
      601 ataaatgata acaccaatag ctatgtaaac agcggaaagg cccggttgca cggtgtggaa
      661 tttgccggca cattgccgct gtggtcagag gatgtcacgc tgtcactgaa ttacacctgg
      721 acccgaagtg aacaacgtga tggtgataac aaaggtgcgc cgctgagtta tacccctgaa
      781 cacatggtga atgcgaaact gaactggcag atcaccgaag aggtggcatc atggctgggt
      841 gcccgttatc gcgggaaaac accacgtttc acccagaatt attcgtcact gagcgctgta
      901 cagaagaaag tgtatgatga gaaaggagaa tacctgaaag cctggacggt ggtggatgca
      961 ggtctgtcgt ggaagatgac ggatgccctg acgctgaatg ctgcggtgaa taacctgctc
     1021 aacaaggatt acagtgacgt gagcctgtac agtgccggta agagtacgct gtatgccggt
     1081 gattacttcc agacgggatc atcaacaaca ggatatgtga tacctgagcg aaattactgg
     1141 atgtcgctga actatcagtt ctgataataa caaaacgcta tcactgacgg tagaatacgt
     1201 tgccactgca actcctggcg gaacagtggc aacgtattag gttaagtgca tttccgatcc
     1261 gctaatgaga tttcgttacc aacaactaat atcgtcacag gaaatgcacg gattattttt
     1321 aacttatcat ttacatactt gtccagagtg taagcgcacc gtgaaggacg tggggtaaaa
     1381 attagtttac agagagagtg acgttccagg gcaacaactc tttcatgcgg ttggcaggcc
     1441 aggtgttgat tacactgatc acgtggcgta gccacgtttc cggctcgatt ccgttaagtt
     1501 ttgcagctac cgatcaggct gtacatcact gccgcactat cgctcgtcat ctcaaagtcc
     1561 ggtctcgtca gcaggaaggt atcattctct cccgccattt ttccaggggc ccggtcagat
     1621 aagtcccttt gtctatcgct gactcctgac tcataacccg gttagcagaa tgcagggtca
     1681 ccacctcgcc acgaccaaat ccaaataagt caattgcacc ttctcaatcg ccattttgtc
     1741 agtaagcgta cagcctgaac tgatggtatc ttcaccatca atgacaacgg tgatcgcaat
     1801 tttactgacg ttcgccggaa cacgatccag tgctatctca atgctggcct gctgcgaacc
     1861 ggtaacgagc ctgactgccc cctcaggaga agacaaatta ttataaaaga taaagtcaga
     1921 atcgccactg acctttccct gagcattaag catgaacagg caggtatcgg gttcgccttt
     1981 aaagccggat ttggctgggt actgaagatt cagcctgatc gtagattgct gaaggggtat
     2041 gttttgtccg gattgtaaat tcatattaac tctcctgatt tttgattatt attaatgcgc
     2101 agcgtttata tatgttccct aggcttagtt ctggacgctg gatattcggt gaggcgtaaa
     2161 tatggtatga caccattttt cataacgctg aagtttctat acctgttgaa tttgaatttt
     2221 cattgaccgg gtatcttatt ttccagggcc cgttccttca taagtcgcaa aagtaacata
     2281 tatccgaagg gcatgctgtt gatatcagac acggaatact ggctttaacc agcaaccaca
     2341 gataaacccg gggcctgcat aaaaggttca tgccagaatt aacatagctc tgctttctgc
     2401 cccccccctt tcccatattc acccgttgat agcggatcga tacccaaaaa aaaccccggc
     2461 attgccgggg tcagactcag cgtcttttag acattgacgc cgtgctggga agcaagcgct
     2521 gacagaccac cggcaaaacc ctggccaaca gctttaaact tccactcagt accatggcga
     2581 tacagttcac cgaagaccat tgcggtttcg gttgaggcgt cttcagacag atcgaaacgg
     2641 gcaatttccg tcccgttgtc gttgttgtaa acgcgcatga agctgttgct caccatgccg
     2701 aagttttgtt tacgcgcttc tgcatcatag atggtaacgg caaataccag ttttttgatg
     2761 tctgctgaga ctttggtcag atcgattttg acctgctcat cgtcgccgtc gccttcaccg
     2821 gtacggttgt cgccctggtg ctctactgcg ccatcagggc tggttttatt attgaagaaa
     2881 atgaaatggg catctgacag tactttaccg tcttcaccta ctgcgaatac ggaagcgtcc
     2941 agatcaaaac cctgaccatc ggttacacgg gcatcccagc ccaggccaac catagcgaca
     3001 ttcatggttg gtgcttcttt ggtcagagat acgttgccgc cttttacgag agaaactgcc
     3061 atttttagct cctgcaaaca gttgaatgag gctgaattaa cagccccaga tatgaaaagt
     3121 tacttttcga tcaggacgcg ttaatgccgt actgagcaca tacagatgcc agaccaccag
     3181 cataaccctg gcctactgcg cggaatttcc actcaccatt gtggcgatac agctcgccga
     3241 acagcatggc agtctcagtg gacgcatctt cggtcagatc gtagcgagcg acttcagtct
     3301 ggttatcgtc attaaccaga cgaataaacg caccggatac ctgaccaaag ctctggcgac
     3361 gagcctgagc atcgtggatg gtcacaacga agatgatctt gtcaacttca gacgggacgg
     3421 cgtccagttt aattttcagc gattcatcat caccatcgcc ctcaccggtg cggttatcgc
     3481 cggtgtgcgt tacggaaccg tcggatgacg tcaggttgtt atagaagatg aaatctgaat
     3541 cgccgcgcac tttgccgttt gaggccagca ggaatgctga agcatccagg tcaaagtcct
     3601 gaccgtctgt tgaacgcgca tcccagccaa ggcccaccag gacatttttc attgacggag
     3661 ctgctttact cagggagacg ttcccgcctt tggaaagaga aacactcata aaataacctc
     3721 ttcgattagt aattgttcag gttaacactt aaggggatta actccccttt tcctcagatt
     3781 caggtttgcc cgggaacatg acgcttgcga gaatgcccag cgccagtaca cccagtacaa
     3841 catacaggct ggttgttgcc gcgatgctgt aaccatgatg ccagatgtga tcggtcgcat
     3901 tcaggccgag ttttgccgcg atgaagaaca gcagcacgat aacggccttc tccagatgaa
     3961 ccaggtactg tttcagtgcc tcaaggacaa aatacagagt acgcaggccc aggatagcaa
     4021 acatcatggc actatagacg ataagcggtt cacgactgac ggcaatgatt gccggtaccg
     4081 agtcaaacgc gaacattacg tccgagagtt caaccacagc cacacacagg aacagcgggg
     4141 tcgcatacag cgccgctttt ttaccacggc caatggtgac atcgctgttt tctggtttcg
     4201 ccagttcagc atccacttcc ttctggttaa gtaggaaggc atgccctctg agcttcggcc
     4261 agatagggaa gaagcgttta accatacggt aagccagatg ctgggagtaa tcctcaattt
     4321 catcatcgtc atcaccgctt ttaagcatca tgaccgccgt ccaggcaacg ataatagcga
     4381 agacaacttc aacgtacggc cccagactca gcaggctcgt accgatggcg acaaagatgc
     4441 ccctgaagac gatggcacca atgatccccc agtagagaac acggtggcga taacgatccg
     4501 gaacggcgaa ccaggagaag attgccatca tgacgaacag gttatcgacc gacagcactt
     4561 tctccagcgc ataacccgtg acaaacagac tggcaacctc agcaccgtgg tggatataga
     4621 ggaaaccggc aaatgccatc gcaaccacaa cccagaatac ggaccagagc gcagcactct
     4681 tcagcgagat tggcttgtca tcacggtgca taaacaggtc aataaagatg gcaccgactg
     4741 aaagcgcaat aaaaacaatg accgtttcag tcgggaagcc gatgtgtgtg gaaaccatat
     4801 tttaccctta gggatgtgga tcagaggcca aattcagccg ggtcgaagcc caacgcgata
     4861 gcgatctcgc gggaggccag tttctcgtcc tgatcgaagt taccgtcact tttcgcaacg
     4921 gcaataccaa cacgtaaggc cagctgagcg gcctcaggct gatctttcag cgccaggatg
     4981 tatttcatgg tttcgccctt gccaatttca acatcgaaat cgaagcttga aaccagttta
     5041 ttgaagaact caatcacctc attggtatcg aagaccttca gctcttctga agagcgcaga
     5101 aagcccatca ttttctgctt ttcttccgaa cttacgccgt cactcgatac ggcaatacgg
     5161 gcacatacag caacggtgcc ctgcatgaat tttttgtttt tgaaacggcc aacctggcgg
     5221 gtcagttcgt cacggcctga gttaatggca cctttaactt tgtcgaaaaa gctcatacct
     5281 gtttcctttg ttgctgtgtt atttagaacc ggcgctccag cgaaatcccc agccaaatgc
     5341 ccggtccatt tcgtcctggc ctttgaagta ctggttaatt cgctcgactt taattgcgcc
     5401 gttttcgttt acgagtctgg caatggcgca caatgtgcga cggttctcac cttcggtcag
     5461 gcgggtctca atgggcggtt gatccggcac atgaatggtg actacaccat cagtcttgtc
     5521 ccagctggga acaccttcat aaataaaggc gtaaatcagc acttcgcgga tatgcttcca
     5581 ttcacgtcca ttgatatgca gccactcgcc gtctgataca tcacccgtcc ggtcatcgcc
     5641 cttgagctga acatacggtt catcgcgata atcaccgaat gcattaccga gcgcctgaat
     5701 gaccgattta tacccgtcct gaagctcaac aaaggccccc agatccaggt cgattccttt
     5761 ggagccgaac atacctgcaa agcctgattt gctactgccc cggtgccagt tcaggttgat
     5821 ccggatttca ccgaagttat cccgtttggt caggctgatt gcaggcttct cttttgtcag
     5881 cgataccttg ctcaggctga tttttgtctc aactggcgga ggggttacaa ccggaggagt
     5941 tggggctgcg ggagccggtt cgtcggcaat atttacgccg aaatgctctg ccagcggttt
     6001 aagtccaccg ttgaacccct gcgcaacgaa gcggaatttc cagtcattat tacgacggta
     6061 aaattccccc agaattaagg ctgcttcctg acggccgctc agttcgacac tgccgctaac
     6121 caggccggta gccccttgct ccacgtcgat ggaaagatta cgaagaccgg acaccgtctg
     6181 cccaccgtca caggtcacgg tgaacgcaat tttctggaca tcaggtttca gccggttgag
     6241 cgcgacagta aaggttgagt actggccttc actgaccagg ctgacggtac catcatcgtt
     6301 acgtggctga ccataaaaca ccatgtccgc atcaccctgc accttcccgt cggcatacag
     6361 gcgaaacgcc gaagcatcca cctggccgcc cgaggtgatc cggacccgca actcctgggc
     6421 tggaacgggg gcattgcccc ccggcgtcag gttcattagc gaataaccgc tgaaacgata
     6481 tcggagtgca tatcgtcaat tgtgcggccg cgacaggcat ggccaagggc ggtaaagtcc
     6541 cagttgccgt tattacggcg cagcgaagaa ataacgatcc cggtatgtga gccctgctca
     6601 gtcagcttgt agcgagccag ctctttgccg gtttgatcga caacgcggca gaaagcgttt
     6661 tcgacgtcat tgaacgactg accacggaag ctgtttaccg taaacgccag gtattcaacg
     6721 tttgcaggca ggcgggagag gttgacctta atcacttcat cgtcaccgtc accctcaccg
     6781 gtcaggttat cgccactgtg cacaacagcg ccgcaggtgg atttcagttt gcggaaccag
     6841 atggtgtcaa ttgttttacc tgtgctgtcc attaatacac agccagcatc gagatcgatt
     6901 gagtcgttgc ctccaaacaa tcctccaaga agaccttttt tcttaaccgg atcccaaccg
     6961 agaccgaaat gaagctggct taatgcagat gattctttgc tgagtgatac cgtctggttc
     7021 ttaactaatg aaaccatgtt ttattcc
//
LOCUS       DAZKHF020000221         7044 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2201S31241
            SAMN43267800-rid25106413.denovo.223, whole genome shotgun sequence.
ACCESSION   DAZKHF020000221 DAZKHF020000000
VERSION     DAZKHF020000221.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267800
            Sequence Read Archive: SRR30307263
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 7044)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7044)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 7044)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350581.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350581.2
            Genome Coverage       :: 151x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:21:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,652
            CDSs (total)                      :: 5,557
            Genes (coding)                    :: 5,320
            CDSs (with protein)               :: 5,320
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 237
            CDSs (without protein)            :: 237
            Pseudo Genes (ambiguous residues) :: 0 of 237
            Pseudo Genes (frameshifted)       :: 102 of 237
            Pseudo Genes (incomplete)         :: 130 of 237
            Pseudo Genes (internal stop)      :: 52 of 237
            Pseudo Genes (multiple problems)  :: 41 of 237
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7044
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267800-rid25106413.denovo.223"
                     /serotype="O 26:H11"
                     /isolate="2201S31241"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-01"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            6..293
                     /gene="yafQ"
                     /locus_tag="ACHOSC_RS18895"
                     /old_locus_tag="pgaptmp_003779"
     CDS             6..293
                     /gene="yafQ"
                     /locus_tag="ACHOSC_RS18895"
                     /old_locus_tag="pgaptmp_003779"
                     /EC_number="3.1.26.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system mRNA interferase
                     toxin YafQ"
                     /protein_id="HGU4549629.1"
                     /translation="MRNLNQRDIEYSGQFSKDVKLAQKRHKDMNKLKYLMTLLINNAL
                     PLPAVYKDHPLQGSWKGYRDAHVEPDWILIYKLTDKLLRFERTGTHAALFG"
     gene            449..1189
                     /gene="dpaA"
                     /locus_tag="ACHOSC_RS18900"
                     /old_locus_tag="pgaptmp_003780"
     CDS             449..1189
                     /gene="dpaA"
                     /locus_tag="ACHOSC_RS18900"
                     /old_locus_tag="pgaptmp_003780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan meso-diaminopimelic acid protein
                     amidase"
                     /protein_id="HGU4549630.1"
                     /translation="MRKIALILAMLLIPCVSFAGLLGSSSSTTPVSKEYKQQLMGSPV
                     YIQIFKEERTLDLYVKMGEQYQLLDSYKICKYSGGLGPKQRQGDFKSPEGFYSVQRNQ
                     LKPDSRYYKAINIGFPNAYDRAHGYEGKYLMIHGDCVSIGCYAMTNQGIDEIFQFVTG
                     ALVFGQPSVQVSIYPFRMTDANMKRHKYSNFKDFWEQLKPGYDYFEQTRKPPTVSVVN
                     GRYVVSKPLSHEVVQPQLASNYTLPEAK"
     gene            complement(1160..1927)
                     /gene="yafJ"
                     /locus_tag="ACHOSC_RS18905"
                     /old_locus_tag="pgaptmp_003781"
     CDS             complement(1160..1927)
                     /gene="yafJ"
                     /locus_tag="ACHOSC_RS18905"
                     /old_locus_tag="pgaptmp_003781"
                     /EC_number="2.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0006541 - glutamine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class II glutamine amidotransferase"
                     /protein_id="HGU4549631.1"
                     /translation="MCELLGMSANVPTDICFSFTGLVQRGGGTGPHKDGWGITFYEGK
                     GCRTFKDPQPSFNSPIAKLVQDYPIKSCSVVAHIRQANRGEVALENTHPFTRELWGRN
                     WTYAHNGQLTGYKSLETGNFRPVGETDSEKAFCWLLHKLTQRYPRTPGNMAAVFKYIA
                     SLADELRQKGVFNMLLSDGRYVMAYCSTNLHWITRRAPFGVATLLDQDVEIDFSSQTT
                     PNDVVTVIATQPLTGNETWQKIMPGEWRLFCLGERVV"
     gene            complement(2133..2711)
                     /gene="lpcA"
                     /locus_tag="ACHOSC_RS18910"
                     /old_locus_tag="pgaptmp_003782"
     CDS             complement(2133..2711)
                     /gene="lpcA"
                     /locus_tag="ACHOSC_RS18910"
                     /old_locus_tag="pgaptmp_003782"
                     /EC_number="5.3.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008968 - D-sedoheptulose 7-phosphate
                     isomerase activity [Evidence IEA];
                     GO_process: GO:1901135 - carbohydrate derivative metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-sedoheptulose 7-phosphate isomerase"
                     /protein_id="HGU4549632.1"
                     /translation="MYQDLIRNELNEAAETLANFLKDDANIHAIQRAAVLLADSFKAG
                     GKVLSCGNGGSHCDAMHFAEELTGRYRENRPGYPAIAISDVSHISCVGNDFGFNDIFS
                     RYVEAVGREGDVLLGISTSGNSANVIKAIAAAREKGMKVITLTGKDGGKMAGTADIEI
                     RVPHFGYADRIQEIHIKVIHILIQLIEKEMVK"
     gene            2951..5395
                     /gene="fadE"
                     /locus_tag="ACHOSC_RS18915"
                     /old_locus_tag="pgaptmp_003783"
     CDS             2951..5395
                     /gene="fadE"
                     /locus_tag="ACHOSC_RS18915"
                     /old_locus_tag="pgaptmp_003783"
                     /EC_number="1.3.8.7"
                     /EC_number="1.3.8.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414756.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003995 - acyl-CoA dehydrogenase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0033539 - fatty acid beta-oxidation using
                     acyl-CoA dehydrogenase [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase FadE"
                     /protein_id="HGU4549633.1"
                     /translation="MMILSILATVVLLGALFYHRVSLFISSLILLAWTASLGVAGLWS
                     AWVLVPLAIILVPFNFAPMRKSMISAPVFRGFRKVMPPMSRTEKEAIDAGTTWWEGDL
                     FQGKPDWKKLHNYPQPRLTAEEQAFLDGPVEEACRMANDFQITHELADLPPELWAYLK
                     EHRFFAMIIKKEYGGLEFSAYAQSRVLQKLSGVSGILAITVGVPNSLGPGELLQHYGT
                     DEQKDHYLPRLARGQEIPCFALTSPEAGSDAGAIPDTGIVCMGEWQGQQVLGMRLTWN
                     KRYITLAPIATVLGLAFKLSDPEKLLGGAEDLGITCALIPTTTPGVEIGRRHFPLNVP
                     FQNGPTRGKDVFVPIDYIIGGPKMAGQGWRMLVECLSVGRGITLPSNSTGGVKSVALA
                     TGAYAHIRRQFKISIGKMEGIEEPLARIAGNAYVMDAAASLITYGIMLGEKPAVLSAI
                     VKYHCTHRGQQSIIDAMDITGGKGIMLGQSNFLARAYQGAPIAITVEGANILTRSMMI
                     FGQGAIRCHPYVLEEMEAAKNNDVNAFDKLLFKHIGHVGSNKVRSFWLGLTRGLTSST
                     PTGDATKRYYQHLNRLSANLALLSDVSMAVLGGSLKRRERISARLGDILSQLYLASAV
                     LKRYDDEGRNEADLPLVHWGVQDALYQAEQAMDDLLQNFPNRVVAGLLNVVIFPTGRH
                     YLAPSDKLDHKVAKILQVPNATRSRIGRGQYLTPSEHNPVGLLEEALVDVIAADPIHQ
                     RICKELGKNLPFTRLDELAHNALAKGLIDKDEAAILVKAEESRLRSINVDDFDPEELA
                     TKPVKLPEKVRKVEAA"
     gene            complement(5438..5884)
                     /gene="ivy"
                     /locus_tag="ACHOSC_RS18920"
                     /old_locus_tag="pgaptmp_003784"
     CDS             complement(5438..5884)
                     /gene="ivy"
                     /locus_tag="ACHOSC_RS18920"
                     /old_locus_tag="pgaptmp_003784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308274.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ivy family C-type lysozyme inhibitor"
                     /protein_id="HGU4549634.1"
                     /translation="MFKAITTVAALVIATSAMAQDDLTISSLAKGETTKAAFNQMVQG
                     HKLPAWVMKGGTYTPAQTVTLGDETYQVMSACKPHDCGSQRIAVMWSEKSNQMTGLFS
                     TIDEKTSQEKLTWLNVNDALSIDGKTVLFAALTGSLENHPDGFNFK"
     gene            6065..6832
                     /gene="yafV"
                     /locus_tag="ACHOSC_RS18925"
                     /old_locus_tag="pgaptmp_003785"
     CDS             6065..6832
                     /gene="yafV"
                     /locus_tag="ACHOSC_RS18925"
                     /old_locus_tag="pgaptmp_003785"
                     /EC_number="3.5.1.111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001758752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoglutaramate amidase"
                     /protein_id="HGU4549635.1"
                     /translation="MPGLKITLLQQPLVWMDGPANLRHFDRQLEGITGRDVIVLPEMF
                     TSGFAMEAAASSLAQDDVVNWMTAKAQQCNALIAGSVALQTESGSVNRFLLVEPGGTV
                     HFYDKRHLFRMADEHLHYKAGNARVIVEWRGWRILPLVCYDLRFPVWSRNLNDYDLAL
                     YVANWPAPRSLHWQALLTARAIENQAYVAGCNRVGSDGNGCHYRGDSRVINPQGEIIA
                     TADAHQATRIDAELSMAVLREYRDKFPAWRDADEFRL"
     gene            complement(6953..>7044)
                     /locus_tag="ACHOSC_RS18930"
                     /old_locus_tag="pgaptmp_003786"
     CDS             complement(6953..>7044)
                     /locus_tag="ACHOSC_RS18930"
                     /old_locus_tag="pgaptmp_003786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000027427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4549636.1"
                     /translation="LNTCYIEEVLASCSELGFRTDKMKNLTQI"
BASE COUNT         1666 a         1766 c         1893 g         1719 t
ORIGIN      
        1 gataaattag gaatttaaat caaagggata ttgaatactc gggacaattt tcaaaggatg
       61 taaaacttgc acaaaagcgt cataaggata tgaataaatt gaaatatctt atgacgcttc
      121 ttatcaataa tgctttaccg cttccagctg tttataaaga ccacccgctg caaggttcat
      181 ggaaaggtta tcgcgatgct catgtcgaac cggactggat cctgatttac aaacttaccg
      241 ataaactttt acgatttgag agaactggaa ctcacgcggc gctctttggg taaagcacgc
      301 tgccgatgaa atttgtaaac cctgcgccga caggcttgcg taaaactctt ttggtgataa
      361 tgtgagcctt ccaaatccag actattccga tttgggatct attttgccgt cctggcgtgt
      421 gtaaccgttt tatcaaggaa taagcagtat gcgtaaaatc gcattaattc ttgcgatgtt
      481 gttgatcccg tgcgtttcgt ttgccggttt gctgggcagc agtagctcga ccacgccagt
      541 gagcaaagag tataagcagc agttgatggg atcccctgtc tacatccaga tcttcaagga
      601 agaacgtacg ctcgatctct acgtcaaaat gggcgagcaa tatcagctgc tcgacagtta
      661 taaaatctgt aaatattccg gcggcttagg gccaaaacag cgtcagggcg atttcaaaag
      721 cccggaaggg ttttatagcg tccagcgtaa tcagttaaaa ccagacagcc gttactacaa
      781 agcgattaat attggtttcc ccaatgccta tgaccgtgcg catggttatg aagggaaata
      841 cctgatgatc cacggcgatt gtgtttccat cggctgctac gcaatgacca atcagggtat
      901 tgatgagatc ttccagttcg ttactggtgc gctggtgttt ggtcagccga gcgtgcaagt
      961 gagtatttac ccgttccgca tgaccgacgc caatatgaag cgccataaat attccaactt
     1021 taaggacttc tgggagcagc tgaagccggg ctacgactac tttgagcaaa cccgtaagcc
     1081 accgaccgtt tctgtggtca atggccgtta cgtggtcagc aagccgttaa gccacgaagt
     1141 tgtgcagcca caactggcat caaactacac gctccccgag gcaaaataag cgccattcgc
     1201 ctggcataat cttttgccag gtttcattgc ccgtcagcgg ctgtgttgca atcaccgtga
     1261 ccacatcatt cggtgtggtc tgcgagctga agtcgatttc cacatcctga tccagcagcg
     1321 ttgccacgcc aaacggcgcg cggcgggtga tccagtgtaa attagtcgag caatacgcca
     1381 tcacatagcg cccgtcagaa agcagcatgt tgaaaacgcc cttctgccgc agttcatccg
     1441 ccagtgaggc gatatattta aacactgccg ccatgttgcc cggtgtgcgc gggtaacgct
     1501 gcgttaattt atgcaggagc cagcagaagg ctttttcgct atcggtttca ccgaccgggc
     1561 ggaagttgcc ggtttccagt gatttgtagc ccgtcagttg tccgttatgg gcataagtcc
     1621 agttacgccc ccataactcg cgggtaaatg ggtgagtatt ttccagcgcc acctcgcccc
     1681 gattagcctg gcgaatatga gccaccaccg aacaggattt tatcgggtag tcctggacaa
     1741 gtttggcgat gggggaatta aagctgggtt gtggatcttt aaatgtgcga cagcctttac
     1801 cttcgtaaaa ggtaatgccc cagccatctt tatgtggccc ggttccacca ccacgctgta
     1861 caagtccggt gaaactaaag cagatatcgg tagggacgtt ggcgctcatc ccgagcaatt
     1921 cgcacatggg taacctccac aacagcggga gcgaatggcc cccggcgaag attgatttaa
     1981 gaattttcag gtcggatgcg gcgtaaacga attattcggc ctacggttcg ggtacagatt
     2041 tgtaggcatg ataagacgcg tcagcatcgc atcaggcatc agcgcacaag tgccggatgc
     2101 ggcgtaaacg ccttatccag cctacgccag acttacttaa ccatctcttt ttcaattaac
     2161 tggatcagga tatggatcac tttaatgtga atctcctgaa tgcggtcggc ataaccaaag
     2221 tgcggtacgc gaatttcgat atccgccgtg ccagccattt tgccgccgtc tttaccggtc
     2281 agggtgatca ctttcatccc cttctcacgc gccgctgcga tcgctttgat cacgtttgca
     2341 gagttaccgg aggtggagat ccccagcagt acatcgcctt cgcgacctac cgcttcaacg
     2401 tagcgggaga aaatatcatt gaaaccgaaa tcattaccga cgcaggaaat atgactaacg
     2461 tcagaaatag caatcgccgg gtagcccgga cggttttcac ggtagcgacc ggtcaactct
     2521 tcggcaaagt gcatagcgtc gcaatgggaa ccgccgttgc cgcaggaaag cactttgcca
     2581 ccggctttaa agctgtctgc taacaggacc gccgcgcgct gaatggcgtg aatattggcg
     2641 tcatctttta aaaagttagc cagcgtttcc gccgcttcgt tcagttcgtt acgaataaga
     2701 tcctggtaca tgaggatatc cttcagcata aatgtaatag acaaaatgca gtgtaccgga
     2761 taccgccaaa agcgagaagt acgggcaggt gctatgacca ggactttttg acctgaagtg
     2821 cggataaaaa cagcaacaat gtgagctttg ttgtaattat attgtaaaca cattgctaaa
     2881 tgtttttaca tccactacaa ccatatcatc acaagtggtc agacctccta caagtaaggg
     2941 gcttttcgtt atgatgattt tgagtattct cgctacggtt gtcctgctcg gcgcgttgtt
     3001 ctatcaccgc gtgagcttat ttatcagcag tctgattttg ctcgcctgga cagcctccct
     3061 cggcgttgct ggtctgtggt cggcgtgggt actggtgcct ctggccatta tcctcgtgcc
     3121 atttaacttt gcgcctatgc gtaagtcgat gatttccgcg ccggtatttc gcggtttccg
     3181 taaggtgatg ccgccgatgt cgcgcactga gaaagaagcg attgatgcgg gcaccacctg
     3241 gtgggagggc gacttgttcc agggcaagcc ggactggaaa aagctgcata actatccgca
     3301 gccgcgcctg accgccgaag agcaagcgtt tctcgacggc ccggtagaag aagcctgccg
     3361 gatggcgaat gatttccaga tcacccatga gctggcggat ctgccgccgg agttgtgggc
     3421 gtaccttaaa gagcatcgtt tcttcgcgat gatcatcaaa aaagagtacg gcgggctgga
     3481 gttctcggct tatgcccagt ctcgcgtgct gcaaaaactc tccggcgtga gcgggatcct
     3541 ggcgattacc gtcggcgtgc caaactcatt aggcccgggc gaactgttgc aacattacgg
     3601 cactgacgag cagaaagatc actatctgcc gcgtctggcg cgtggtcagg agatcccctg
     3661 ctttgcactg accagcccgg aagcgggttc cgatgcgggc gcgattccgg acaccgggat
     3721 tgtctgcatg ggcgaatggc agggccagca ggtgctgggg atgcgtctga cctggaacaa
     3781 acgctacatt acgctggcac cgattgcgac tgtgcttggg ctggcgttta aactctccga
     3841 cccggaaaag ctgctgggtg gcgcagaaga tttaggtatt acctgtgcgc tgatcccgac
     3901 caccacgccg ggcgtggaaa ttggtcgtcg ccacttcccg ctgaacgtac cgttccagaa
     3961 cggaccgacg cgcggtaaag atgtcttcgt accgatcgat tacatcatcg gtgggccgaa
     4021 aatggccggg caaggctggc ggatgctggt ggagtgcctc tcggtaggcc gcggcatcac
     4081 cctgccttcc aactcaaccg gcggcgtgaa atcggtagcg ctggcaacgg gcgcgtatgc
     4141 tcacattcgc cgtcagttca aaatctctat cggtaagatg gaagggattg aagagccgct
     4201 ggcgcgtatt gccggtaatg cctacgtgat ggatgctgcg gcatcgctga ttacctacgg
     4261 cattatgctc ggcgaaaaac ctgccgtgct gtcggctatc gttaagtatc actgtaccca
     4321 ccgcgggcag cagtcgatta ttgatgcgat ggatatcact ggtggcaaag gcattatgct
     4381 cgggcagagc aacttcctgg cgcgtgctta ccagggcgca ccgattgcca tcaccgttga
     4441 aggggccaac attctgaccc gcagcatgat gatcttcgga caaggagcga ttcgttgcca
     4501 tccgtacgtg ctggaagaga tggaagcagc gaagaacaat gacgtcaacg cgttcgataa
     4561 actgttgttc aaacatatcg gtcacgtcgg tagcaacaaa gttcgcagct tctggctggg
     4621 cctgacgcgc ggtttaacca gcagcacgcc aaccggcgat gccactaaac gctactatca
     4681 gcacctgaac cgcctgagcg ccaacctcgc cctgctttct gatgtctcga tggctgtgct
     4741 gggcggcagc ctgaaacgtc gtgagcgcat ctcggcacgt ctgggggata ttttaagcca
     4801 gctttacctc gcctctgccg ttctgaagcg ttatgacgac gaaggccgta atgaagccga
     4861 cctgccgctg gtgcactggg gcgtacaaga tgcgctgtat caggctgaac aggcgatgga
     4921 tgatttactg caaaacttcc cgaaccgcgt ggttgccggg ctgctgaatg tggtgatctt
     4981 cccgaccgga cgtcattatc tggcaccttc tgacaagctg gatcataaag tggcgaagat
     5041 tttacaagtg ccgaacgcca cccgttcccg cattggtcgc ggtcagtacc tgacgccgag
     5101 cgagcataat ccggttggct tgctggaaga ggcgctggtg gatgtgattg ccgccgaccc
     5161 aattcatcag cggatctgta aagagctggg taaaaacctg ccgtttaccc gtctggatga
     5221 actggcgcac aacgcgctgg cgaaggggct gattgataaa gatgaagccg ctattctggt
     5281 gaaagctgaa gaaagccgtc tgcgcagtat taacgttgat gactttgatc cggaagagct
     5341 ggcgacgaag ccggtaaagt tgccggagaa agtgcggaaa gttgaagccg cgtaattgaa
     5401 taacggagcc gaaaggctcc gtttctttat ccgctaatta tttaaaatta aagccatccg
     5461 gatggttttc caggctgccg gtcaacgccg cgaacagcac cgttttacca tcaatcgaaa
     5521 gcgcatcgtt cacattcagc caggtgagtt tctcttgcga cgttttctca tcaatagtcg
     5581 agaacagccc cgtcatctga ttagatttct cggaccacat cacagcgata cgttgcgagc
     5641 cacagtcatg cggtttgcac gcgctcatca cctgatacgt ctcatctccc aacgttacgg
     5701 tttgtgcggg agtataagta ccgcctttca tcacccaggc aggcagctta tgcccttgta
     5761 ccatctgatt aaatgcagct ttggtggttt cgccctttgc aaggctgcta atggttaaat
     5821 catcctgcgc cattgcactg gtggcgatga ccagagcggc gactgtcgtt attgccttaa
     5881 acatcattcc tcccgagctt atcctgccca tgttattaac cttctaaagc ctggcgataa
     5941 cacgataatt tgcaaatcag atatttccaa tatcagcatt tgttatcaat ttattctgcg
     6001 ctaaggcaac tgcctgataa ggtgttaaag ttataaaacc cttcgcataa aggagttgta
     6061 caccgtgcct ggtttgaaga ttacgctttt gcagcaacca ctggtgtgga tggatggccc
     6121 tgccaacctg cgtcattttg atcgtcaact ggaaggtatt accgggcgcg atgtgatcgt
     6181 tctaccggag atgtttacca gcggctttgc catggaagcg gcagcttcgt cgctagcaca
     6241 agatgacgta gtgaactgga tgacagctaa ggcgcagcag tgcaatgcgc tgattgcagg
     6301 cagtgttgca ttacaaacgg agtctggttc ggttaaccgc tttttgctgg ttgagccggg
     6361 cggcacggta catttttatg ataagcgtca tctgttccgc atggcagatg agcatctaca
     6421 ttataaagcg ggcaatgcgc gagtgattgt ggaatggcgc ggctggcgta ttttgccgct
     6481 ggtgtgctac gacttacgtt ttcctgtgtg gtcgcgcaat ctcaacgatt atgacctcgc
     6541 cctgtacgtc gccaactggc ctgctccgcg ctctctgcac tggcaggcat tgctgacggc
     6601 ccgcgcgatt gagaatcagg cgtatgtggc gggatgcaat cgcgtcggca gcgatggcaa
     6661 cggctgccat tatcgcggtg acagccgggt gattaatcca caaggcgaga ttatcgctac
     6721 tgccgacgcg catcaggcaa cgcgcattga tgccgagctg tcgatggcgg tgttaaggga
     6781 atatcgcgat aagtttccgg catggcggga tgcggatgag tttaggttgt agtgagtggg
     6841 ggctgtattt agggcgggga attcagaaag ggatgtttag tgtgctgagc gagagttaaa
     6901 taataaaaaa ctgggaagat ttaataatcc ccccgcaggg caagagcatg aattaaattt
     6961 gtgttaaatt tttcatttta tcagttcgaa acccaagctc tgagcaggat gcaagcactt
     7021 cttctatata acatgtgttt aacg
//
LOCUS       DAZKHF020000222         7039 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2201S31241
            SAMN43267800-rid25106413.denovo.224, whole genome shotgun sequence.
ACCESSION   DAZKHF020000222 DAZKHF020000000
VERSION     DAZKHF020000222.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267800
            Sequence Read Archive: SRR30307263
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 7039)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7039)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 7039)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350581.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350581.2
            Genome Coverage       :: 151x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:21:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,652
            CDSs (total)                      :: 5,557
            Genes (coding)                    :: 5,320
            CDSs (with protein)               :: 5,320
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 237
            CDSs (without protein)            :: 237
            Pseudo Genes (ambiguous residues) :: 0 of 237
            Pseudo Genes (frameshifted)       :: 102 of 237
            Pseudo Genes (incomplete)         :: 130 of 237
            Pseudo Genes (internal stop)      :: 52 of 237
            Pseudo Genes (multiple problems)  :: 41 of 237
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7039
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267800-rid25106413.denovo.224"
                     /serotype="O 26:H11"
                     /isolate="2201S31241"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-01"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            79..1413
                     /gene="adeQ"
                     /locus_tag="ACHOSC_RS18935"
                     /old_locus_tag="pgaptmp_003787"
                     /gene_synonym="yicO"
     CDS             79..1413
                     /gene="adeQ"
                     /locus_tag="ACHOSC_RS18935"
                     /old_locus_tag="pgaptmp_003787"
                     /gene_synonym="yicO"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418120.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenine permease AdeQ"
                     /protein_id="HGU4549637.1"
                     /translation="MNNDNTDYVSNESGTLSRLFKLPQHGTTVRTELIAGMTTFLTMV
                     YIVFVNPQILGAAQMDPKVVFVTTCLIAGIGSIAMGIFANLPVALAPAMGLNAFFAFV
                     VVGAMGISWQTGMGAIFWGAVGLFLLTLFRIRYWMISNIPLSLRIGITSGIGLFIALM
                     GLKNTGVIVANKDTLVMIGDLSSHGVLLGILGFFIITVLSSRHFHAAVLVSIVVTSCC
                     GLFFGDVHFSGVYSIPPDISGVIGEVDLSGALTLELAGIIFSFMLINLFDSSGTLIGV
                     TDKAGLIDSNGKFPNMNKALYVDSVSSVAGAFIGTSSVTAYIESTSGVAVGGRTGLTA
                     VVVGVMFLLVMFFSPLVAMVPPYATAGALIFVGVLMTSSLARVNWDDFTESVPAFITT
                     VMMPFTFSITEGIALGFMSYCIMKVCTGRWRDLNLCVVVVAALFALKIILVD"
     gene            1466..1918
                     /gene="yicN"
                     /locus_tag="ACHOSC_RS18940"
                     /old_locus_tag="pgaptmp_003788"
     CDS             1466..1918
                     /gene="yicN"
                     /locus_tag="ACHOSC_RS18940"
                     /old_locus_tag="pgaptmp_003788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418119.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1198 domain-containing protein"
                     /protein_id="HGU4549638.1"
                     /translation="MIWIMLATLAVVFVVGFRVLTSGARKAIRRLSDRLNIDVVPVES
                     MVDQMGKSAGDEFLRYLHRPDESHLQNAAQVLLIWQIVIVDGSEQNLLQWHRILQKAR
                     LAAPITDAQVRLALGFLRETEPEMQDINAFQMRYNAFFQPAEGVHWLH"
     gene            2129..3319
                     /gene="nepI"
                     /locus_tag="ACHOSC_RS18945"
                     /old_locus_tag="pgaptmp_003789"
     CDS             2129..3319
                     /gene="nepI"
                     /locus_tag="ACHOSC_RS18945"
                     /old_locus_tag="pgaptmp_003789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418118.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015211 - purine nucleoside transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015860 - purine nucleoside transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="purine ribonucleoside efflux pump NepI"
                     /protein_id="HGU4549639.1"
                     /translation="MSEFIAENRGADAITRPNWSAVFSVAFCVACLIIVEFLPVSLLT
                     PMAQDLGISEGVAGQSVTVTAFVAMFASLFITQTIQATDRRYVVILFAVLLTLSCLLV
                     SFANSFSLLLIGRACLGLALGGFWAMSASLTMRLVPPRTVPKALSVIFGAVSIALVIA
                     APLGSFLGELIGWRNVFNAAAVMGVLCIFWIIKSLPSLPGEPSHQKQNTFRLLQRPGV
                     MAGMIAIFMSFAGQFAFFTYIRPVYMNLAGFSVDGLTLVLLSFGIASFIGTSLSSFIL
                     KRSVKLALAGAPLILAVSALVLTLWGSDKIVATGVAIIWGLTFALVPVGWSTWITRSL
                     ADQAEKAGSIQVAVIQLANTCGAAIGGYALDNIGLTSPLMLSGTLMLLTALLVTAKVK
                     MKKS"
     gene            complement(3360..3653)
                     /gene="yicS"
                     /locus_tag="ACHOSC_RS18950"
                     /old_locus_tag="pgaptmp_003790"
     CDS             complement(3360..3653)
                     /gene="yicS"
                     /locus_tag="ACHOSC_RS18950"
                     /old_locus_tag="pgaptmp_003790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YicS"
                     /protein_id="HGU4549640.1"
                     /translation="MKPTTLLLIFTFFAMPGIVYAESPFSSLQSAKEKTTVLQDLRKI
                     CTPQASLSDEAWEKLMLSDENNKQHIHEAIVAMERNNQSNYWEALGKVECPDM"
     gene            3875..4693
                     /gene="nlpA"
                     /locus_tag="ACHOSC_RS18955"
                     /old_locus_tag="pgaptmp_003791"
     CDS             3875..4693
                     /gene="nlpA"
                     /locus_tag="ACHOSC_RS18955"
                     /old_locus_tag="pgaptmp_003791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein NlpA"
                     /protein_id="HGU4549641.1"
                     /translation="MKLTTHHLRTGAALLLAGILLAGCDQSSSDAKHIKVGVINGAEQ
                     DVAEVAKKVAKEKYGLDVELVGFSGSLLPNDATNHGELDANVFQHRPFLEQDNQAHGY
                     KLVAVGNTFVFPMAGYSKKIKTVAQIKEGATVAIPNDPTNLGRALLLLQKEKLITLKE
                     GKGLLPTALDITDNPRHLQIMELEGAQLPRVLDDPKVDVAIISTTYIQQTGLSPVHDS
                     VFIEDKNSPYVNILVAREDNKNAENVKEFLQSYQSPEVAKAAETIFNGGAVPGW"
     gene            complement(4697..5620)
                     /gene="yicL"
                     /locus_tag="ACHOSC_RS18960"
                     /old_locus_tag="pgaptmp_003792"
     CDS             complement(4697..5620)
                     /gene="yicL"
                     /locus_tag="ACHOSC_RS18960"
                     /old_locus_tag="pgaptmp_003792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxylate/amino acid/amine transporter"
                     /protein_id="HGU4549642.1"
                     /translation="MGSTRKGMLNVLIAAVLWGSSGVCAQYIMEQSQMSSQFLTMTRL
                     IFAGLILLTLSFVHGDKIFSIINNHKDAISLLIFSVVGALTVQLTFLLTIEKSNAATA
                     TVLQFLSPTIIVAWFSLVRKSRPGILVFCAILTSLVGTFLLVTHGNPTSLSISPAALF
                     WGIASAFAAAFYTTYPSTLIARYGTLPVVGWSMLIGGLILLPFYARQGTNFVVNGSLI
                     LAFFYLIVIGTSLTFSLYLKGAQLIGGPKASILSCAEPLSSALLSLLLLGITFTLPDW
                     LGTLLILSSVILISMDSRRRARKINRPARHE"
     gene            complement(5731..6915)
                     /gene="setC"
                     /locus_tag="ACHOSC_RS18965"
                     /old_locus_tag="pgaptmp_003793"
     CDS             complement(5731..6915)
                     /gene="setC"
                     /locus_tag="ACHOSC_RS18965"
                     /old_locus_tag="pgaptmp_003793"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005351 - carbohydrate:proton symporter
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar efflux transporter"
                     /protein_id="HGU4549643.1"
                     /translation="MQKTATTPSKILDLTAAAFLLVAFLTGIAGALQTPTLSIFLGDE
                     LKARPIMVDFFFTGSAIMGILVSQFLARHSDKQGDRKLLILLCCLFGVLACTLFAWNR
                     NYFILLSTGVLLSSFASTANPQMFALAREHADRTGRETVMFSTFLRAQISLAWVIGPP
                     LAYELAMGFSFKVMYLTAAIAFVVCGLIVWLFLPSIQRNIPVVTQPVEILPSTHRKRD
                     TRLLFVVCSMMWAANNLYMINMPLFIIDELHLTDKLAGEMICIAAGLEIPMMLIAGYY
                     MKRIGKRLLMLIAIVSGMCFYASVLMATTPAVELELQILNAIFLGILCGIGMLYFQDL
                     MPEKIGSATTLYANTSRVGWIIAGSVDGIMVEIWSYHALFWLAIGMLGIAMICLLFIK
                     DI"
BASE COUNT         1762 a         1587 c         1777 g         1913 t
ORIGIN      
        1 gtgataatta tcactgaacc gttgttgtca tttgttttac aaaagcaagg gattaaatct
       61 cctccaatgg acaaaaaaat gaataatgac aataccgatt acgtgagtaa tgaatcaggg
      121 acgctttcgc gattatttaa actacctcag catgggacca ccgtccgcac agaattgatt
      181 gcggggatga ccactttttt aaccatggtg tacatcgttt ttgtgaaccc gcaaatcctc
      241 ggcgcggcac aaatggaccc gaaagtggtg tttgttacca cctgtttgat tgccggtatc
      301 ggcagtattg cgatggggat atttgctaac ttacccgtgg cgctggctcc ggcaatgggg
      361 ctgaacgcct tctttgcctt cgtggtcgtg ggggcgatgg gcatctcctg gcagaccggg
      421 atgggcgcaa tattctgggg cgcagttgga ctatttttgc tcacgctgtt tcgtatccgg
      481 tactggatga tctccaacat tcccttaagt ttacgtattg gtatcaccag cggaattgga
      541 ttatttatcg ccttaatggg attaaaaaat actggcgtta ttgtcgccaa taaagacacg
      601 ctggtgatga ttggcgattt aagttctcac ggcgtgttgt taggtatttt agggtttttt
      661 attataaccg tgttgtcatc acgtcatttt catgccgcgg tgctggtttc tattgtggtg
      721 acgtcttgct gtggattatt tttcggtgat gttcatttta gcggcgtcta ttccattccg
      781 cctgatatta gcggcgtcat tggtgaagta gatttgagcg gcgcgttaac acttgaactc
      841 gccggtatca ttttctcctt tatgctgatc aacctatttg attcatcagg aacattaatt
      901 ggtgtaactg ataaagcggg cttaatagat agtaacggta aattccccaa tatgaataag
      961 gcgctgtatg ttgatagcgt cagttcggtg gcgggggcgt ttatcggcac ctcgtctgtt
     1021 actgcctata ttgaaagtac ttctggtgtg gcagtcggtg gtcgcacggg gctgactgcg
     1081 gttgtggttg gcgttatgtt cctgttagtt atgttcttct caccgctggt ggcgatggtt
     1141 cctccttacg caaccgccgg agcgttaatc tttgttggcg tgctgatgac ttcgagcctg
     1201 gcgcgcgtta actgggatga ttttaccgaa tcggtgcctg cgtttattac cacggtgatg
     1261 atgcccttta ctttctcgat caccgaaggg attgcactcg gctttatgtc gtactgcatc
     1321 atgaaagtat gcaccgggcg ctggcgcgat ctgaacctgt gtgtggtggt ggtcgcagct
     1381 ctgtttgcac tgaagattat tctggtggat tagtaactgg cgagggtttc cagatatcat
     1441 gagttctgat tacgcaggag aactcatgat ctggataatg ctcgccacgc tggcggtagt
     1501 gtttgtggtt ggttttcggg tgctgacatc cggggccaga aaagcgattc gccgtctcag
     1561 cgatcggctg aacatcgatg tcgtacccgt ggagtcgatg gtcgatcaaa tgggaaagtc
     1621 agctggtgac gaatttttac gttatttgca tcgtccggat gagtcgcacc tgcaaaacgc
     1681 cgcgcaggtg ttgctcatct ggcaaattgt cattgtcgat ggtagcgaac agaacctgct
     1741 gcaatggcat cggattttac aaaaagctcg cctcgccgcg ccgattaccg acgctcaggt
     1801 caggctggcg ctaggttttc tgcgcgaaac cgaacctgaa atgcaggata ttaatgcttt
     1861 tcagatgcgc tataacgcgt tctttcagcc tgccgagggc gttcactggt tgcattgaaa
     1921 ccgtttcgtc ttatcagcct acagaccgcg cctgacctgt cgggtggata agacgttcac
     1981 gccgcatccg gtaatctttg ccctgttccg tttcgatctt aactccagct caagcaacac
     2041 cgtaaaagtt gcaaaaacgt taaatgcgtc acacatttca atgttaaagt ggtcggcttt
     2101 tcccctgaaa catgccacgg gtaacaccat gagtgaattt attgccgaaa accgcggcgc
     2161 ggatgccatc acccgaccga actggtcagc cgttttctcg gtggcgtttt gtgtcgcctg
     2221 tctgattatc gttgagtttt tgcccgtcag tttgttgacg ccaatggccc aggatttagg
     2281 catttcggaa ggggttgccg ggcaatcggt gaccgtgacc gcctttgtgg caatgtttgc
     2341 cagtttgttt attacccaga caattcaggc tactgaccgc cgctacgttg ttattttgtt
     2401 tgccgttttg ctgacgctct cctgcttgct ggtttccttt gctaactcat tcagtttgct
     2461 tttaatcggt cgtgcctgtc tggggctggc gctgggcggg ttctgggcga tgtcggcgtc
     2521 gctgaccatg cgtctggtgc cgccgcgtac ggtgccgaag gcgctgtcgg tgatcttcgg
     2581 cgcggtttct attgcgctgg tgattgccgc gccgttgggc agttttttag gcgagcttat
     2641 cggttggcgc aatgtcttta atgcggcggc ggtgatgggc gtgctgtgta ttttctggat
     2701 tatcaaatca ttgccttcac tgccaggcga accctcgcat cagaaacaaa atactttccg
     2761 cttattacaa cgtccgggtg tgatggcagg gatgatcgcc atcttcatgt ctttcgccgg
     2821 gcagtttgct ttcttcacat atattcgccc ggtgtatatg aacctggcgg gattcagcgt
     2881 ggatggctta acgctggtgc tgttgagttt tggcattgcc agctttatcg gtacgtcgct
     2941 ttcgtcattc attcttaaac gttcggtaaa actggcctta gcaggcgcgc cgttaatact
     3001 ggctgtgagt gcgttggtgc tgacgttgtg gggaagcgat aaaatcgttg ctaccggcgt
     3061 ggcgattatc tgggggctaa cttttgcatt ggttcccgtc ggctggtcaa cgtggatcac
     3121 ccgctcgctg gccgatcagg cagaaaaagc cgggtctatt caggtggcgg ttattcagct
     3181 tgctaatacc tgtggcgcgg caatcggcgg ttatgcgctg gataatattg gtctgacttc
     3241 gccgttgatg ttgtccggca cattgatgtt gctgactgca ttgttggtta ctgcaaaggt
     3301 gaaaatgaag aaatcctgat atcaggtaaa gaagcgttgg tcagtagttg ttgctgttct
     3361 tacatatccg ggcattctac cttacccaac gcttcccaat aattgctctg attattacgt
     3421 tccatcgcca cgatggcctc gtgtatatgt tgtttattgt tttcatctga caacattaac
     3481 ttttcccacg cttcatctga taatgacgcc tgtggcgtgc aaattttacg taaatcttgt
     3541 aataccgtgg ttttctcttt tgcggactgc agtgagctaa aaggcgactc ggcgtatacg
     3601 atgcctggca tagcgaaaaa agtgaaaata agcagtagtg tcgttggttt catggattca
     3661 accttttaca aatgtgtatc gaatgaagca taagtgtagt ttgctttcat cgctctcatc
     3721 atgctgtacg aaacgcgctt gcgtggtcag taagaagtgc cagactttat attccacttt
     3781 tattcctttt tattcttatc gatagcgttt cgttttttaa accgcagcga ccttaccgct
     3841 atagtcaggt aatcattaat aaaaggataa aaaaatgaaa ctgacaacac atcatctacg
     3901 gacaggggcc gcattattgc tggccggaat tctgctggca ggttgcgacc agagtagcag
     3961 cgatgcaaaa cacattaaag ttggcgttat aaatggcgca gaacaagatg tcgcggaagt
     4021 cgctaaaaag gtggcaaaag agaaatatgg cctcgatgtt gaactggtag gatttagcgg
     4081 ttcgttgcta ccgaacgatg cgacaaatca tggcgaactt gatgcgaatg tcttccagca
     4141 tcgacccttt cttgaacaag ataatcaggc gcatggttat aaactggtag cggtgggaaa
     4201 tacgtttgtc ttccctatgg cgggttattc caaaaaaatc aaaaccgtgg cgcaaataaa
     4261 agaaggcgcg acagtggcga tccccaatga cccgaccaac cttggtcgcg cacttttact
     4321 gttgcaaaaa gagaagctaa ttactctgaa agagggaaaa gggttattac ctaccgcgct
     4381 ggatataacc gataatcctc gtcatttgca gattatggaa ctcgaggggg cgcagctccc
     4441 gcgcgtactg gatgatccca aagttgatgt agcgattatc agcaccactt acattcagca
     4501 gacagggctt tctccggtgc acgacagcgt atttattgaa gataagaatt cgccgtatgt
     4561 gaatattttg gtggcacggg aagataataa gaatgcagaa aacgtgaagg aatttctgca
     4621 atcttatcaa tcacccgaag tcgctaaagc ggcagaaacc atttttaacg gtggcgcggt
     4681 gcctggctgg taattctcac tcatgccgcg ccggacgatt tatttttctg gcgcggcgac
     4741 gggaatccat tgaaatcaaa attaccgatg acagaattag cagcgttccc agccagtccg
     4801 gtaatgtgaa cgtgatcccc agcaacagca aagagagtag cgcgctactt aatggttctg
     4861 cacagctcaa aatgctggct tttggaccgc caattaattg tgctcctttc aggtacagac
     4921 taaatgtcag ggacgtacca atgacgatca aataaaaaaa cgccagaatc aaactgccgt
     4981 taacgacaaa gtttgttcct tgtctggcat aaaaaggcaa cagaatcaga ccgccaatca
     5041 gcatactcca gccgacgact ggtaacgtgc catagcgggc aattagtgtt gagggatagg
     5101 tggtatagaa tgcagcagca aatgccgagg caatgcccca gaacaacgcg gcaggagaga
     5161 tcgataatga cgtcggatta ccgtgtgtca ccaataaaaa agtcccgacc agcgatgtca
     5221 aaatagcgca gaaaaccaga atgcccgggc gcgatttacg caccagtgag aaccaggcga
     5281 cgataatcgt cggtgagagg aattgcagca ccgttgccgt ggctgcgttc gatttttcga
     5341 tggttagcaa aaaagtgagc tgtacagtta gcgcgccaac cacggaaaaa atcagcaggc
     5401 taatggcatc tttatgattg ttaataatag aaaagatttt atcgccatga acaaatgaca
     5461 gcgtcagtag aatcaaaccg gcgaatatca aacgcgtcat agtcaaaaac tgcgacgaca
     5521 tctggctttg ctccatgatg tattgcgcac agacccctga acttccccac aacacggcgg
     5581 caatcagaac gttcagcatc ccctttctgg tggaacccat ttttccctct gcatactgtt
     5641 ttgtcgttta tgtatgcagg catgatagca aaaatggcga ggatggctct gtttgcgcca
     5701 cgccgatttt tcttagccca gactggtgag ctaaatatct ttaataaaca gcaggcaaat
     5761 catcgcaata cccaacatcc ctatcgccag ccagaacaac gcatggtagc tccagatttc
     5821 aaccataatt ccgtcaacag agccggcgat aatccagccg acgcgtgaag tatttgcata
     5881 taacgttgtc gcagagccta ttttttcagg catcaagtcc tgaaaataaa gcatgccgat
     5941 accacagaga ataccaagga agatggcatt aagaatttgc aattccagct caaccgccgg
     6001 agtcgtcgcc atgagtacgc tggcgtaaaa acacattcca ctcacgatag caatgagcat
     6061 taatagtcgc ttgccaatac gtttcatgta atagcctgcg attaacatca tcggaatttc
     6121 cagaccggca gcgatacaaa tcatttctcc agccagttta tcggttagat gcagttcatc
     6181 aataataaat agcggcatat ttatcatgta gagattattc gccgcccaca tcattgaaca
     6241 gaccacaaaa agtagccgcg tatcccgctt cctgtgggtg gagggtaaaa tttctacggg
     6301 ttgggtaacg acaggaatat ttctttgtat tgatggcaaa aacaaccaga ctatcagtcc
     6361 gcaaacaaca aatgcgatgg cagcggtgag atacatcact ttaaaactaa atcccattgc
     6421 cagttcataa gcgagcggtg gcccgataac ccaggcaagc gagatctgag cacgtaaaaa
     6481 tgtactgaac atgaccgtct cacggcctgt tctgtcggcg tgttcacggg cgagggcgaa
     6541 catttgcggg tttgcggtgg aagcaaaact actcagaagt acgcccgttg agaggagaat
     6601 gaagtagttg cgattccacg caaaaagcgt gcaggccagc actccaaata agcaacatag
     6661 cagaatcagt aatttacggt cgccttgttt atcggagtgc cttgccagaa attgactgac
     6721 cagaattccc ataatagcgc taccggtgaa gaaaaaatct accattatag gacgggcttt
     6781 cagttcatct ccgaggaata tacttagggt aggagtctga agagcgcccg caatacccgt
     6841 cagaaaggcg acaagtaaaa atgccgcggc agtgagatca agtatttttg atggagtggt
     6901 agccgttttt tgcattcttc tgaatcaccg ggcgacaaaa tataactgag ttacaaataa
     6961 gtaccagtcg aatgagtatt gtattaagaa tttttgagta tcgctgatac aaccagaaag
     7021 caatgctagc gcaatatta
//
LOCUS       DAZKHF020000223         7029 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2201S31241
            SAMN43267800-rid25106413.denovo.225, whole genome shotgun sequence.
ACCESSION   DAZKHF020000223 DAZKHF020000000
VERSION     DAZKHF020000223.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267800
            Sequence Read Archive: SRR30307263
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 7029)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7029)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 7029)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350581.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350581.2
            Genome Coverage       :: 151x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:21:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,652
            CDSs (total)                      :: 5,557
            Genes (coding)                    :: 5,320
            CDSs (with protein)               :: 5,320
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 237
            CDSs (without protein)            :: 237
            Pseudo Genes (ambiguous residues) :: 0 of 237
            Pseudo Genes (frameshifted)       :: 102 of 237
            Pseudo Genes (incomplete)         :: 130 of 237
            Pseudo Genes (internal stop)      :: 52 of 237
            Pseudo Genes (multiple problems)  :: 41 of 237
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7029
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267800-rid25106413.denovo.225"
                     /serotype="O 26:H11"
                     /isolate="2201S31241"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-01"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            <1..820
                     /gene="hlyE"
                     /locus_tag="ACHOSC_RS18970"
                     /old_locus_tag="pgaptmp_003794"
     CDS             <1..820
                     /gene="hlyE"
                     /locus_tag="ACHOSC_RS18970"
                     /old_locus_tag="pgaptmp_003794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415700.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hemolysin HlyE"
                     /protein_id="HGU4549644.1"
                     /translation="DQVIPWQTFDETIKELSRFKQEYSQAASVLVGDIKTLLMDSQDK
                     YFEATQTVYEWCGVATQLLAAYILLFDEYNEKKASAQKDILIKVLDDGITKLNEAQKS
                     LLVSSQSFNNASGKLLALDSQLTNDFSEKSSYFQSQVDKIRKEAYAGAAAGVVAGPFG
                     LIISYSIAAGVVEGKLIPELKNKLKSVQNFFTTLSNTVKQANKDIDAAKLKLTTEIAA
                     IGEIKTETETTRFYVDYDDLMLSLLKEAAKKMINTCNEYQKRHGKKTLFEVPEV"
     gene            complement(1027..1473)
                     /gene="ycgN"
                     /locus_tag="ACHOSC_RS18975"
                     /old_locus_tag="pgaptmp_003795"
     CDS             complement(1027..1473)
                     /gene="ycgN"
                     /locus_tag="ACHOSC_RS18975"
                     /old_locus_tag="pgaptmp_003795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309703.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcgN family cysteine cluster protein"
                     /protein_id="HGU4549645.1"
                     /translation="MSDVPFWQSKTLDEMSDAEWESLCDGCGQCCLHKLMDEDTDEIY
                     FTNVACRQLNIKTCQCRNYERRFEFEPDCIKLTRENLPTFEWLPMTCAYRLLAEGKDL
                     PAWHPLLTGSKAAMHGERISVRHIAVKESEVIDWQDHILNKPDWAQ"
     gene            complement(1565..2224)
                     /gene="ycgM"
                     /locus_tag="ACHOSC_RS18980"
                     /old_locus_tag="pgaptmp_003796"
     CDS             complement(1565..2224)
                     /gene="ycgM"
                     /locus_tag="ACHOSC_RS18980"
                     /old_locus_tag="pgaptmp_003796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarylacetoacetate hydrolase family protein"
                     /protein_id="HGU4549646.1"
                     /translation="MYQHHNWQGALLDYPVSKVVCVGSNYAKHIKEMGSAVPEEPVLF
                     IKPETALCDLRQPLAIPSDFGSVHHEVELAVLIGATLRQATEEHVRKAIAGYGVALDL
                     TLRDVQGKMKKAGQPWEKAKAFDNSCPLSGFIPAAEFTGDPQNTTLGLSVNGEQRQQG
                     TTADMIHKIVPLIAYMSKFFTLKAGDVVLTGTPDGVGPLQSGDELTVTFDGHSLATRV
                     L"
     gene            complement(2296..2589)
                     /locus_tag="ACHOSC_RS18985"
                     /old_locus_tag="pgaptmp_003797"
     CDS             complement(2296..2589)
                     /locus_tag="ACHOSC_RS18985"
                     /old_locus_tag="pgaptmp_003797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309701.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcgL domain-containing protein"
                     /protein_id="HGU4549647.1"
                     /translation="MFCVIYRSSKRDQTYLYVEKKDDFSRVPEELMKGFGQPQLAMIL
                     PLDGRKKLVNADIEKVKLALTEQGYYLQLPPPPEDLLKQHLSVMGQKTDDTNK"
     gene            complement(2601..2759)
                     /locus_tag="ACHOSC_RS18990"
                     /old_locus_tag="pgaptmp_003798"
     CDS             complement(2601..2759)
                     /locus_tag="ACHOSC_RS18990"
                     /old_locus_tag="pgaptmp_003798"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4549648.1"
                     /translation="MAFLRSVKSRRGIFAGGITYKGAWTQQTVEIYSWLACGAGFIDV
                     SLCQNLAS"
     gene            2830..3231
                     /gene="pliG"
                     /locus_tag="ACHOSC_RS18995"
                     /old_locus_tag="pgaptmp_003799"
     CDS             2830..3231
                     /gene="pliG"
                     /locus_tag="ACHOSC_RS18995"
                     /old_locus_tag="pgaptmp_003799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="g-type lysoyme inhibitor PliG"
                     /protein_id="HGU4549649.1"
                     /translation="MKIKSISKAVLLFALLTSTSFAAGKNVNVEFRKGHSSAQYSGEI
                     KGYDYDTYTFYAKKGQKVHVSISNEGADTYLFGPGIDDSVDLSRYSPELDSHGQYSLP
                     ASGKYELRVLQTRNDARKNKTKKYNVDIQIK"
     gene            complement(3334..3702)
                     /gene="ycgJ"
                     /locus_tag="ACHOSC_RS19000"
                     /old_locus_tag="pgaptmp_003800"
     CDS             complement(3334..3702)
                     /gene="ycgJ"
                     /locus_tag="ACHOSC_RS19000"
                     /old_locus_tag="pgaptmp_003800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcgJ family protein"
                     /protein_id="HGU4549650.1"
                     /translation="MNMMRIIYIGLSGVGMMFSSMASGHDASGLQSPACGVVCDPYIC
                     VNSDGISPELTRKYLGEKAAENLQSLQGYDPSEFTFANGVFCDVKEKLCRDDRYFGVD
                     GKRSGKINQTTTKMLFMCRE"
     gene            4222..4917
                     /gene="minC"
                     /locus_tag="ACHOSC_RS19005"
                     /old_locus_tag="pgaptmp_003801"
     CDS             4222..4917
                     /gene="minC"
                     /locus_tag="ACHOSC_RS19005"
                     /old_locus_tag="pgaptmp_003801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004857 - enzyme inhibitor activity
                     [Evidence IEA];
                     GO_process: GO:0000918 - division septum site selection
                     [Evidence IEA];
                     GO_process: GO:1901891 - regulation of cell septum
                     assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="septum site-determining protein MinC"
                     /protein_id="HGU4549651.1"
                     /translation="MSNTPIELKGSSFTLSVVHLHEAEPKVIHQALEDKIAQAPAFLK
                     HAPVVLNVSALEDPVNWSAMHKAVSATGLRVIGVSGCKDAQLKAEIEKMGLPILTEGK
                     EKAPRPAPAPQAPAQNTTPVTKTRLIDTPVRSGQRIYAPQCDLIVTSHVSAGAELIAD
                     GNIHVYGMMRGRALAGASGDRETQIFCTNLMAELVSIAGEYWLSDQIPAEFYGKAARL
                     QLVENALTVQPLN"
     gene            4941..5753
                     /gene="minD"
                     /locus_tag="ACHOSC_RS19010"
                     /old_locus_tag="pgaptmp_003802"
     CDS             4941..5753
                     /gene="minD"
                     /locus_tag="ACHOSC_RS19010"
                     /old_locus_tag="pgaptmp_003802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020801817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septum site-determining protein MinD"
                     /protein_id="HGU4549652.1"
                     /translation="MARIIVVTSGKGGVGKTTSSAAIATGLAQKGKKTVVIDFDIGLR
                     NLDLIMGCERRVVYDFVNVIQGDATLNQALIKDKRTENLYILPASQTRDKDALTREGV
                     AKVLDDLKAMDFEFIVCDSPAGIETGALMALYFADEAIITTNPEVSSVRDSDRILGIL
                     ASKSRRAENGEEPIKEHLLLTRYNPGRVSRGDMLSMEDVLEILRIKLVGVIPEDQSVL
                     RASNQGEPVILDINADAGKAYADTVERLLGEERPFRFIEEEKKGFLKRLFGG"
     gene            5757..6023
                     /gene="minE"
                     /locus_tag="ACHOSC_RS19015"
                     /old_locus_tag="pgaptmp_003803"
     CDS             5757..6023
                     /gene="minE"
                     /locus_tag="ACHOSC_RS19015"
                     /old_locus_tag="pgaptmp_003803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0032955 - regulation of division septum
                     assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division topological specificity factor
                     MinE"
                     /protein_id="HGU4549653.1"
                     /translation="MALLDFFLSRKKNTANIAKERLQIIVAERRRSDAEPHYLPQLRK
                     DILEVICKYVQIDPEMVTVQLEQKDGDISILELNVTLPEAEELK"
     gene            complement(6395..6739)
                     /locus_tag="ACHOSC_RS19020"
                     /old_locus_tag="pgaptmp_003804"
                     /pseudo
     CDS             complement(6395..6739)
                     /locus_tag="ACHOSC_RS19020"
                     /old_locus_tag="pgaptmp_003804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311542.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter outer membrane beta-barrel
                     domain-containing protein"
     gene            complement(6774..6893)
                     /locus_tag="ACHOSC_RS19025"
                     /old_locus_tag="pgaptmp_003805"
     CDS             complement(6774..6893)
                     /locus_tag="ACHOSC_RS19025"
                     /old_locus_tag="pgaptmp_003805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001131457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase"
                     /protein_id="HGU4549654.1"
                     /translation="MPLSYAGYHHFIMMSLVSGEHNTPALSLQDESPGLVPAG"
     gene            complement(6854..>7029)
                     /gene="ymgJ"
                     /locus_tag="ACHOSC_RS19030"
                     /old_locus_tag="pgaptmp_003806"
     CDS             complement(6854..>7029)
                     /gene="ymgJ"
                     /locus_tag="ACHOSC_RS19030"
                     /old_locus_tag="pgaptmp_003806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="protein YmgJ"
                     /protein_id="HGU4549655.1"
                     /translation="YSSATITLVILFCRRPHCRKISVRQYYLKRVIFYPTTLQRTACK
                     KCLFLMRDIIISS"
BASE COUNT         1991 a         1680 c         1558 g         1800 t
ORIGIN      
        1 cgatcaggtc atcccctggc agacctttga tgaaaccata aaagagttaa gtcgctttaa
       61 acaggagtat tcacaggcag cctccgtttt agtcggcgat attaaaacct tacttatgga
      121 tagccaggat aagtattttg aagcaaccca aacagtgtat gaatggtgtg gtgttgcgac
      181 gcaattgctc gcagcgtata ttttgctatt tgatgagtac aatgagaaga aagcatccgc
      241 ccagaaagac attctcatta aggtactgga tgacggcatc acgaagctga atgaagcgca
      301 aaaatccctg ctggtaagct cacaaagttt caacaacgct tccgggaaac tgctggcgtt
      361 agatagccag ttaaccaatg atttttcaga aaaaagcagc tatttccagt cacaggtaga
      421 taaaatcagg aaggaagcgt atgccggtgc cgcagccggt gtcgtcgccg gtccatttgg
      481 attaatcatt tcctattcta ttgctgcggg cgtagttgaa gggaaactga ttccagaatt
      541 gaagaacaag ttaaaatctg tgcagaattt ctttaccacc ctgtctaaca cggttaaaca
      601 agcgaataaa gatatcgatg ccgccaaatt gaaattaacc accgaaatag ccgccatcgg
      661 ggagataaaa acggaaactg aaacaaccag attctatgtt gattatgatg atttaatgct
      721 ttctttgcta aaagaagcgg caaaaaaaat gattaacacc tgtaatgagt atcagaaaag
      781 acacggtaaa aagacactct ttgaggtacc tgaagtctga taagcgatta ttctctccat
      841 gtactcaagg tataaggttt atcacattta aaaacacaga cgttttttaa agattgagag
      901 ttacctgttg ataataaggt atcgggtaac tctcattatg acagagcgct ttcaaaaaca
      961 gggctacgaa atggcgagca tccagacgta tattaagaaa gcccccatca acatctatta
     1021 aatttatcac tgcgcccagt caggtttatt taagatatga tcctgccagt caatcacttc
     1081 tgattctttc actgcgatat gacgcacaga gatacgttca ccatgcattg ccgctttcga
     1141 acccgtaagt agcggatgcc acgcaggtaa atctttacct tccgccagca accgataagc
     1201 gcaggtcatt ggcagccatt cgaatgttgg cagattttca cgggttaatt taatgcagtc
     1261 gggttcaaac tcgaaacgac gttcgtagtt ccgacattga caggttttaa tattgagctg
     1321 gcgacaggcg acgttagtga agtagatttc gtcggtgtct tcatccatca gtttatgcag
     1381 gcaacactga ccgcaaccat cacacaacga ctcccattcc gcatcgctca tttcgtccag
     1441 ggttttactt tgccagaaag gtacatcgct catcaggtgt tccgccatta cgttaaaacg
     1501 caccttataa ccagtctggc acagcgatgc aagttttgcc gccgctttca ggcggcaaaa
     1561 agtattacaa aacgcgagtt gccaaagaat gcccatcgaa agtgactgtc agctcatcac
     1621 cgctttgcaa cgggccgacg ccatcaggcg tgcctgtcag cacaacgtca ccggccttga
     1681 gggtaaaaaa cttgctcata taagcgatca gcggaacaat tttatggatc atgtccgcag
     1741 tcgtaccttg ctgacgttgc tcgccgttta cgctcaggcc cagcgttgta ttttgcgggt
     1801 cgccggtaaa ttccgccgcg ggaataaacc cggaaagcgg acaagagtta tcaaacgctt
     1861 tagccttttc ccacggctgc ccggctttct tcatttttcc ctgaacatca cgcaatgtca
     1921 gatcgagcgc cacgccataa ccggcaatgg ctttgcggac atgctcttcc gtagcctgac
     1981 gcagcgtcgc gccaatcaac accgccagtt cgacttcatg atgaactgaa ccgaaatcgg
     2041 atgggatcgc caatggctgc cgcagatcgc acagtgccgt ttctggttta ataaacagca
     2101 ctggctcttc gggcactgcg ctgcccatct ctttaatatg tttggcatag ttactgccaa
     2161 cacagactac tttactcacc ggataatcca gcagcgcacc ttgccagttg tgatgttgat
     2221 acataacgtt cccctcacct tatcgtggat atgaaaaccc gcgccgacaa agataatgcc
     2281 accgccggat atcggttatt tgttagtgtc gtctgttttc tgccccatga cggaaagatg
     2341 ttgcttcagc aaatcttcgg gtggtggcgg taactgcaaa tagtaacctt gctcggttaa
     2401 tgccagttta accttttcaa tatcagcatt gaccagtttc ttccgcccat ccaacggcag
     2461 aatcatcgct aactgaggct gaccaaaacc tttcatcagt tcctcaggaa cacgcgaaaa
     2521 atcgtctttt ttttcgacat ataaataggt ctggtcacgc ttgctgcttc gataaatcac
     2581 acaaaacata cttttactct ttaagatgcc aggttttggc ataagctaac atcaatgaaa
     2641 cccgcgccac aagccagcca gctgtagatt tctacggttt gttgagtcca tgccccttta
     2701 taagtaatcc ccccggcaaa aatgccgcgc cgggatttta ccgaacgcag gaaagccact
     2761 ggaattctcc cgttagggat tgttatatta taacagttca tcgtactcat tctgaacagg
     2821 agactaccaa tgaaaatcaa gagcatcagt aaggctgtat tgctgtttgc actgctaacc
     2881 tccacgagct ttgctgcggg taagaatgtc aatgttgagt tcagaaaagg ccatagcagc
     2941 gctcagtact ccggcgaaat aaagggatac gattacgata catatacctt ctatgccaaa
     3001 aaaggtcaga aagtgcatgt gagtatctct aatgaaggcg ctgatactta cctcttcggg
     3061 ccaggaatcg atgattcagt tgatttatcc agatattccc cagaactcga tagtcacgga
     3121 caatactcac tccctgcttc tggcaaatat gagctgcgag tgttgcaaac ccgaaacgac
     3181 gcccgtaaaa acaaaactaa aaaatataac gtcgatattc aaattaagta aatgtcaacc
     3241 gacggggact ctccccgtca taaacgttaa cgccccactc ctctctgtcg ctcaagaggt
     3301 taatgacgaa tagaacataa tgtaaacaag acatcactca cgacacataa ataacatctt
     3361 tgtggtggtt tgattgattt ttccactccg cttaccatct acaccaaaat aacgatcatc
     3421 acgacataat ttttctttaa catcgcaaaa tacaccgtta gcgaatgtaa attcgctggg
     3481 atcgtagcct tgtaatgatt gtaagttttc agcggctttt tcgccgagat atttccttgt
     3541 taactctggg gaaatgccat ctgagttcac acaaatatac gggtcgcaaa caacgccgca
     3601 cgccggagat tgaagtccgc tagcgtcatg tccagacgcc attgatgaga acatcatccc
     3661 cacacctgac aatccgatat aaataatacg catcatattc atagcacatc ctgataatcc
     3721 aaccgtacaa cacttatgct catttcgtca tcaatccgta atcaaaaccg ctgacctgga
     3781 cgagttacga tgacctgaca gaagtatcag acgtcttctg cggtttctat gatagagcaa
     3841 ttctgtggcg gatattaaga taacgggtat ctgttcttta atgatgccgc aaaaaatgga
     3901 aagttaagaa agcctggcgt ggtgaaaaaa tacacttgcc tcaatactgg atatttaaac
     3961 aggtctggag atgctgtcac gattagggtg cggaaaaagg aagcgtaaat aagaagaatc
     4021 aaaagtacga cggcaatggg ttgattgaca agggtatttt ttaagctatg aatcagcgcc
     4081 atttatcaca gaatagactt ttactctgaa taaatgggag ggtgacttgc ctcaatataa
     4141 tccagactat aacatgcctt atagtcttcg gaacatcatc gcgcgctggc gatgattaat
     4201 agctaattga gtaaggccag gatgtcaaac acgccaatcg agcttaaagg cagtagcttc
     4261 actttatctg tggttcatct gcatgaggca gaacctaagg ttatccatca ggcgctggaa
     4321 gacaaaatcg ctcaggcccc cgcattttta aaacatgccc ccgttgtact caacgtcagt
     4381 gcactggaag acccggtaaa ctggtcagcg atgcataagg cggtttcggc aaccggtttg
     4441 cgggttattg gcgtaagtgg ctgcaaagat gcgcaactta aagccgaaat tgaaaaaatg
     4501 gggctgccta tcctgacgga aggaaaggaa aaagcgccac gtccagctcc cgcaccgcag
     4561 gctccagcgc aaaatacaac gccggtcaca aaaacgcgtt taatagatac cccggtgcgt
     4621 tccggtcagc gtatttatgc tccacaatgt gatctgattg ttacaagcca cgttagcgct
     4681 ggggccgaat tgattgccga tggtaacatt catgtctatg gcatgatgcg cggtcgtgcg
     4741 ctggcagggg caagtggtga ccgggaaacg caaatatttt gtacgaacct gatggcggaa
     4801 ctggtgtcca tcgcaggtga atactggctg agtgatcaaa tcccagcaga attttatggc
     4861 aaagcggcgc gactgcagtt agtcgaaaac gctttgaccg ttcaaccgtt aaattgatcc
     4921 ctttttaaca aggaatttct atggcacgca ttattgttgt tacttcgggc aaagggggtg
     4981 ttggtaagac aacctccagc gcggccatcg ccactggttt ggcccagaag ggaaagaaaa
     5041 ctgtcgtgat agattttgat atcggcctgc gtaatctcga cctgattatg ggttgtgaac
     5101 gccgggtcgt ttacgatttc gtcaacgtca ttcagggcga tgcaacgcta aatcaggcgt
     5161 taattaaaga taagcgtact gaaaatctct atattcttcc ggcatcgcaa acacgcgata
     5221 aagatgccct cacccgtgaa ggggtcgcca aagttcttga tgatctgaaa gcgatggatt
     5281 ttgaatttat cgtttgtgac tccccggcag ggattgaaac cggtgcgtta atggcactct
     5341 attttgcaga cgaagccatt attaccacca acccggaagt ctcctcagta cgcgactctg
     5401 accgtatttt aggcattctg gcgtcgaaat cacgccgcgc agaaaatggc gaagagccta
     5461 ttaaagagca cctgctgtta acgcgctata acccaggccg cgtaagcaga ggtgacatgc
     5521 tgagcatgga agatgtgctg gagatcctgc gcatcaaact cgtcggcgtg atcccagagg
     5581 atcaatcagt attgcgcgcc tctaaccagg gtgaaccggt cattctcgac attaacgccg
     5641 atgcgggtaa agcctacgca gataccgtag aacgtctgtt gggagaagaa cgtcctttcc
     5701 gcttcattga agaagagaag aaaggcttcc tcaaacgctt gttcggagga taagttatgg
     5761 cattactcga tttctttctc tcgcggaaga aaaacacagc caacattgca aaagaacggc
     5821 tgcagattat tgttgctgaa cgccgtcgca gcgatgcaga accgcattat ctgccgcagt
     5881 tgcgtaaaga tattcttgag gtcatttgta aatacgtaca aattgatcct gagatggtaa
     5941 ccgtacagct tgagcaaaaa gatggcgata tttctattct tgagctgaac gtgaccttac
     6001 cggaagcaga agagctgaaa taagcccgct gtaaaaacgc atttatcttc aaggcagagt
     6061 catttctgcc ttgagttttt catccctctc atccacgttg tggtaaagcg gcgagtattc
     6121 ttgctgatac tcctcattgc tatttccacc tcccccttta caccttaagg ctgttcccac
     6181 gcacagaaca aaatgtttat gcctggttga gtaaataacc tcattgttag tgtggataca
     6241 ctgggggatg ataaccccgc aatttcatta ttcattgttc tcccatagga tgaggtttcc
     6301 cgccgtaact ggcgggcttt ttttgcccca gaattttccc ttttagcccc ctgtaaacga
     6361 aaactgcgcc gaagcgcagt ttaatcgttg atggtcagaa cgaccagttc acaccagcca
     6421 ccgcattcca cggggattcc acaccggcac catggctata ccccaccccg aaatgcccgc
     6481 ttaacgtact gctgaatgag gctttaatac ctgcctggta tattccacgt ctgcccgaca
     6541 aatcattgac aaaattaccg tcactattca ctttcacccg attatcatcg acaaattctt
     6601 tgctcacagc cgccttcagg cagggttcaa tttccatacc gttccccaga cgcatgttgt
     6661 aactcagcgt tgcacccagt tcacgatata tactgcgggt atcgactgat ttcgattcca
     6721 tgccattgga taaatgatag tagcgtctaa gtgacctaag ctctgctgca acatcagccc
     6781 gcaggcacca gaccagggga ttcatcctga agagacagcg caggtgtatt gtgttcaccg
     6841 ctcaccaaag acatcatgat gaaatgatga tatcccgcat aagaaagagg cattttttac
     6901 acgcagtgcg ctgaagtgta gttggataaa aaatcactct tttcagataa tactgtcgta
     6961 cgcttatctt tcgacaatga ggccgtcggc aaaataaaat gactaatgta atcgttgctg
     7021 atgaatatc
//
LOCUS       DAZKHF020000224         7027 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2201S31241
            SAMN43267800-rid25106413.denovo.226, whole genome shotgun sequence.
ACCESSION   DAZKHF020000224 DAZKHF020000000
VERSION     DAZKHF020000224.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267800
            Sequence Read Archive: SRR30307263
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 7027)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7027)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 7027)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350581.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350581.2
            Genome Coverage       :: 151x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:21:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,652
            CDSs (total)                      :: 5,557
            Genes (coding)                    :: 5,320
            CDSs (with protein)               :: 5,320
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 237
            CDSs (without protein)            :: 237
            Pseudo Genes (ambiguous residues) :: 0 of 237
            Pseudo Genes (frameshifted)       :: 102 of 237
            Pseudo Genes (incomplete)         :: 130 of 237
            Pseudo Genes (internal stop)      :: 52 of 237
            Pseudo Genes (multiple problems)  :: 41 of 237
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7027
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267800-rid25106413.denovo.226"
                     /serotype="O 26:H11"
                     /isolate="2201S31241"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-01"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            263..1300
                     /gene="dusA"
                     /locus_tag="ACHOSC_RS19035"
                     /old_locus_tag="pgaptmp_003807"
     CDS             263..1300
                     /gene="dusA"
                     /locus_tag="ACHOSC_RS19035"
                     /old_locus_tag="pgaptmp_003807"
                     /EC_number="1.3.1.-"
                     /EC_number="1.3.1.91"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0017150 - tRNA dihydrouridine synthase
                     activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0002943 - tRNA dihydrouridine synthesis
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA dihydrouridine(20/20a) synthase DusA"
                     /protein_id="HGU4549656.1"
                     /translation="MHGNSEMQKINQTSAMPEKTDVHWSGRFSVAPMLDWTDRHCRYF
                     LRLLSRNTLLYTEMVTTGAIIHGKGDYLAYSEEEHPVALQLGGSDPAALAQCAKLAEA
                     RGYDEINLNVGCPSDRVQNGMFGACLMGNTQLVADCVKAMRDVVSIPVTVKTRIGIDD
                     QDSYEFLCDFINTVSGKGECEMFIIHARKAWLSGLSPKENREIPPLDYPRVYQLKRDF
                     PHLTMSINGGIKSLEEAKAHLQHMDGVMVGREAYQNPGILAAVDREIFGSSDTDADPV
                     AVVRAMYPYIERELSQGTYLGHITRHMLGLFQGIPGARQWRRYLSENAHKAGADINVL
                     EHALKLVADKR"
     gene            1434..1676
                     /gene="pspG"
                     /locus_tag="ACHOSC_RS19040"
                     /old_locus_tag="pgaptmp_003808"
     CDS             1434..1676
                     /gene="pspG"
                     /locus_tag="ACHOSC_RS19040"
                     /old_locus_tag="pgaptmp_003808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313059.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="envelope stress response protein PspG"
                     /protein_id="HGU4549657.1"
                     /translation="MLELLFVIGFFVMLMVTGVSLLGIIAALVVATAIMFLGGMLALM
                     IKLLPWLLLAIAVVWVIKAIKAPKVPKYQRYDRWRY"
     gene            complement(1842..2825)
                     /locus_tag="ACHOSC_RS19045"
                     /old_locus_tag="pgaptmp_003809"
     CDS             complement(1842..2825)
                     /locus_tag="ACHOSC_RS19045"
                     /old_locus_tag="pgaptmp_003809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinone oxidoreductase"
                     /protein_id="HGU4549658.1"
                     /translation="MATRIEFHKHGGPEVLQAVEFTPADPAENEIQVENKAIGINFID
                     TYIRSGLYPPPSLPSGLGTEAAGIVSKVGSGVKHIKAGDRVVYAQSALGAYSSVHNIN
                     ADKAAILPAAISFEQAAASFLKGLTVYYLLRKTYEIKPDEQFLFHAAAGGVGLIACQW
                     AKALGAKLIGTVGTAQKAQSALKAGAWQVINYREENLVERLKEITGGKKVRVVYDSVG
                     RDTWERSLDCLQRRGLMVSFGNSSGAVTGVNLGILNQKGSLYVTRPSLQGYITTREEL
                     TEASNELFSLIASGVIKVDVAEQQKYPLKDAQRAHEILESRATQGSSLLIP"
     gene            2908..4323
                     /gene="dnaB"
                     /locus_tag="ACHOSC_RS19050"
                     /old_locus_tag="pgaptmp_003810"
     CDS             2908..4323
                     /gene="dnaB"
                     /locus_tag="ACHOSC_RS19050"
                     /old_locus_tag="pgaptmp_003810"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replicative DNA helicase"
                     /protein_id="HGU4549659.1"
                     /translation="MAGNKPFNKQQAEPRERDPQVAGLKVPPHSIEAEQSVLGGLMLD
                     NERWDDVAERVVADDFYTRPHRHIFTEMARLQESGSPIDLITLAESLERQGQLDSVGG
                     FAYLAELSKNTPSAANISAYADIVRERAVVREMISVANEIAEAGFDPQGRTSEDLLDL
                     AESRVFKIAESRANKDEGPKNIADVLDATVARIEQLFQQPHDGVTGVNTGYDDLNKKT
                     AGLQPSDLIIVAARPSMGKTTFAMNLVENAAMLQDKPVLIFSLEMPSEQIMMRSLASL
                     SRVDQTKIRTGQLDDEDWARISGTMGILLEKRNIYIDDSSGLTPTEVRSRARRIAREH
                     GGIGLIMIDYLQLMRVPALSDNRTLEIAEISRSLKALAKELNVPVVALSQLNRSLEQR
                     ADKRPVNSDLRESGSIEQDADLIMFIYRDEVYHENSDLKGIAEIIIGKQRNGPIGTVR
                     LTFNGQWSRFDNYAGPQYDDE"
     gene            4376..5473
                     /gene="alr"
                     /locus_tag="ACHOSC_RS19055"
                     /old_locus_tag="pgaptmp_003811"
     CDS             4376..5473
                     /gene="alr"
                     /locus_tag="ACHOSC_RS19055"
                     /old_locus_tag="pgaptmp_003811"
                     /EC_number="5.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001147329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008784 - alanine racemase activity
                     [Evidence IEA];
                     GO_process: GO:0006522 - alanine metabolic process
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine racemase"
                     /protein_id="HGU4549660.1"
                     /translation="MQAATVVINRRALRHNLQRLRELAPASKMVAVVKANAYGHGLLE
                     TARTLPDADAFGVARLEEALRLRAGGITKPVLLLEGFFDARDLPTISAQHFHTAVHNE
                     EQLAALEEASLDEPVTVWMKLDTGMHRLGVRPEQAEAFYHRLTQCKNVRQPVNIVSHF
                     ARADEPKCGATEKQLAIFNTFCEGKPGQRSIAASGGILLWPQSHFDWVRPGIILYGVS
                     PLEDRSTGADFGCQPVMSLTSSLIAVREHKAGEPVGYGGTWVSERDTRLGVVAMGYGD
                     GYPRAAPSGTPVLVNGREVPIVGRVAMDMICVDLGPQAQDKAGDPVILWGEGLPVERI
                     AEMTKVSAYELITRLTSRVAMKYVDLSFCNI"
     gene            5708..6901
                     /gene="tyrB"
                     /locus_tag="ACHOSC_RS19060"
                     /old_locus_tag="pgaptmp_003812"
     CDS             5708..6901
                     /gene="tyrB"
                     /locus_tag="ACHOSC_RS19060"
                     /old_locus_tag="pgaptmp_003812"
                     /EC_number="2.6.1.57"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic amino acid transaminase"
                     /protein_id="HGU4549661.1"
                     /translation="MFQKVDAYAGDPILTLMERFKEDPRSDKVNLSIGLYYNEDGIIP
                     QLQAVAEAEARLNAQPHGASLYLPMEGLNSYRHAIAPLLFGADHPVLQQQRVATIQTL
                     GGSGALKVGADFLKRYFPESGVWVSDPTWENHVAIFAGAGFEVSTYPWYDEATNGVRF
                     NDLLATLKTLPARSIVLLHPCCHNPTGADLTNDQWDAVIEILKSRELIPFLDIAYQGF
                     GAGMEEDAYAIRAIASAGLPALVSNSFSKIFSLYGERVGGLSVLCEDAEAAGRVLGQL
                     KATVRRNYSSPPNFGAQVVAAVLNDEALKASWLAEVEEMRTRILAMRQELVKVLSTEM
                     PERNFDYLLNQRGMFSYTGLSAAQVDRLREEFGVYLIASGRMCVAGLNTANVQRVAKA
                     FAAVM"
BASE COUNT         1647 a         1748 c         1875 g         1757 t
ORIGIN      
        1 caccagagtt taatgtgtga ttttttattt atcgtcgaac ctggattgtt tatcattggc
       61 cttaacaaag ttaacggcta ataagattat ttccatcact tcgtgagagc ttcatgcctt
      121 gagaggatct caattttctt ttgcaatgag acaggcgctt cctgttgtta tggtatagta
      181 ccccgctatt gagcctcctg aatagtgatg ctgaataaca taaccccatg atatatcgat
      241 aaaataatct ctacatttga aaatgcacgg taattctgaa atgcaaaaaa tcaaccaaac
      301 cagcgcaatg cctgaaaaaa ctgacgttca ctggagtggt cggtttagcg ttgcaccaat
      361 gctcgactgg acggacagac attgccgcta tttcttgcgt ctgctttccc gcaatacgtt
      421 gctgtatacc gaaatggtga ccacaggggc gattattcac ggtaaaggtg attacctggc
      481 gtacagtgaa gaagaacatc cggtagcgtt gcaactcggc ggtagcgatc cggcggcgct
      541 ggcacagtgt gcgaagctgg cagaagcgcg tggatatgat gagatcaacc tgaatgtcgg
      601 ctgcccgtct gaccgggtgc agaacggcat gtttggtgcg tgtctgatgg gtaatacgca
      661 gctggttgcc gactgcgtga aagcgatgcg cgatgtggtg tcgattccag tgacggtgaa
      721 aacgcgtatt ggcatcgatg accaggacag ctatgaattt ctctgcgatt tcatcaacac
      781 cgtttccggc aaaggcgagt gtgagatgtt tatcatccac gcacgtaaag cctggctttc
      841 ggggttaagt ccgaaagaaa accgtgaaat cccgccgctc gattatccgc gtgtgtatca
      901 actgaagcgt gactttccgc atctgacaat gtcgattaac ggtggtatca agtcgctgga
      961 agaggccaaa gcacacctgc aacatatgga tggcgtgatg gtcgggcgcg aggcgtatca
     1021 gaatccgggt attctggcgg cggtagaccg ggagatcttt ggttcctcgg ataccgatgc
     1081 cgatccggtg gcggtagtgc gcgccatgta tccgtacatt gagcgtgaac tcagccaggg
     1141 gacgtatctc ggccatatta cccggcatat gttgggcttg ttccagggta ttcctggcgc
     1201 gcggcagtgg cggcgttatt taagtgaaaa tgcccataaa gcgggtgcag acattaatgt
     1261 gctggaacac gcgctcaaac tggtggcgga taagcgttaa cttttcacca aaaagtagtc
     1321 aaattcacca cgccctgcgc accgtcgcgg ggcgttttgc tgttaaatca atagattatt
     1381 tttggcatga ttcttgtaat gccagcaaga gatttcatat ttgggagagc atcatgctgg
     1441 aactactttt tgtgattggc ttttttgtca tgctgatggt caccggcgtt tcgttgctgg
     1501 gcattatcgc cgcgctggtt gtggcgacgg ccattatgtt cctcggcggt atgctggcat
     1561 tgatgattaa gttgctgccg tggttactac tggcgattgc ggtggtgtgg gttattaagg
     1621 cgattaaagc accaaaagtg cctaaatatc agcgttatga ccgctggcgt tactaaggga
     1681 ttgtgcggat gatcacaacc taaggtttta tccttagaac aaaataggaa ttgataatca
     1741 aatctgtcac tattgcgcct ctaacagatt catcgtgctg taccctacat acagccgaac
     1801 tataaaaaga aagggcttcc caggtggaag ccctatttct tttatggaat cagcaggctg
     1861 gaaccttgcg tcgcccggct ttccagaatc tcatgcgcac gctgcgcatc cttcagcgga
     1921 tatttctgct gctcggcgac atcgacctta atcacaccgc tggcaatcaa agagaacagt
     1981 tcattactgg cctcggttaa ttcctcccgc gtggtgatat agccttgcag ggaagggcgt
     2041 gtcacataca acgagccttt ttgattgaga atgcctaagt tcacaccggt aaccgcacct
     2101 gatgagttgc caaaactgac cattaagccg cggcgttgca ggcaatccag cgaccgttcc
     2161 caggtgtctc tgcccacgga atcgtacacc acgcgcactt tcttaccgcc ggtgatctct
     2221 tttaaccgct cgaccagatt ctcttcacga tagttaatta cctgccacgc gcccgctttt
     2281 agcgcgctct gcgctttttg cgcggttcct acggtgccga taagtttcgc gcccagggct
     2341 tttgcccact ggcaggcaat caagccaacg ccgccagccg ctgcgtggaa caggaactgt
     2401 tcatcgggtt taatttcata ggttttacgc agcagataat aaaccgttaa gcctttcagg
     2461 aaggatgccg cagcttgctc aaaagaaatt gccgcaggca gaatcgccgc tttatccgca
     2521 ttaatgttat gcacagagct gtaagcgcct aacgccgact gcgcatagac aacacgatcg
     2581 cctgccttaa tatgctttac accactgccg actttactca cgatgcctgc cgcttcggta
     2641 cctaatccgc tgggtagtga tggcggcggg taaaggccgc tgcggatata cgtgtcgata
     2701 aaattgatgc cgatggcttt attttcgacc tggatttcat tctctgccgg atcggcagga
     2761 gtgaactcta cggcttgaag tacttccggg ccaccgtgct tgtgaaattc aattcgtgtt
     2821 gccatgtgtc ctccagaacg tatcgtcagg ggctgcttca tatggtaaag tttcgaccca
     2881 ttctttatct cggtaactcc attcactatg gcaggaaata aacccttcaa caaacagcag
     2941 gctgaacccc gcgaacgcga tccacaagtt gccgggctga aagtgcctcc gcactcgatc
     3001 gaagcggagc agtcggtgtt gggcggttta atgctggata acgaacgctg ggacgatgta
     3061 gccgagcgtg tggtggcaga cgatttttac acccgcccac accgccatat ctttactgaa
     3121 atggcgcgtt tgcaggaaag cggtagtcct atcgatctga ttacccttgc ggaatcgctg
     3181 gaacgccagg ggcaactcga tagcgtcggt ggttttgctt atctggcaga gctgtcaaaa
     3241 aatacgccaa gtgcggcgaa catcagtgct tatgctgaca tcgtgcgtga acgtgccgtt
     3301 gtccgtgaga tgatctcggt tgcgaatgag attgctgaag ccggttttga tccgcagggg
     3361 cgtaccagcg aagatctgct ggaccttgct gaatcccgcg tctttaaaat tgccgaaagt
     3421 cgtgcaaaca aagacgaagg gccgaagaac atcgccgatg tgctcgacgc aaccgtggcg
     3481 cgtattgagc agttgtttca gcagccacac gatggcgtta ccggagtaaa caccggttat
     3541 gacgatctca acaaaaaaac cgctggcttg cagccgtcgg atttgatcat cgtcgccgcg
     3601 cgtccgtcga tgggtaaaac aacatttgcg atgaacctcg tcgaaaacgc ggcgatgttg
     3661 caggataaac cagtacttat cttctcgctg gagatgcctt cagaacagat tatgatgcgt
     3721 tctctggcgt cgctgtcgcg cgttgaccag actaaaatcc gtaccgggca gctcgatgat
     3781 gaagactggg cgcgcatttc cggcaccatg ggtattttgc tcgaaaaacg caatatctat
     3841 atcgatgact cctccggcct gacgccaacg gaagtgcgtt cccgcgcacg ccgtattgcc
     3901 cgtgaacacg gcggcatcgg gcttatcatg atcgactacc tgcaactgat gcgcgtaccg
     3961 gcgctttccg ataaccgtac gctggaaatt gcagaaatct cccgctcgct gaaagcactg
     4021 gcgaaagaac tgaacgtgcc ggtggtggcg ctgtcccagt tgaaccgttc tctggaacaa
     4081 cgtgccgaca aacgcccggt caactccgac ctgcgtgaat ctggctctat cgagcaggat
     4141 gcggacttga tcatgtttat ctatcgtgat gaggtgtatc acgaaaacag tgatttaaaa
     4201 ggcatcgcgg aaattattat cggtaaacaa cgtaacggcc caatcgggac ggtacgcctg
     4261 acctttaacg gtcaatggtc gcgcttcgac aactatgcgg ggccgcagta cgacgacgaa
     4321 taataattat tttatgaatt aggtaattaa agcaaacact tatcaaggaa cacaaatgca
     4381 agcggcaact gttgtgatta accgccgcgc tctgcgacac aacctgcaac gtcttcgtga
     4441 actggcccct gccagtaaaa tggttgcggt ggtgaaagcg aacgcttatg gtcacggtct
     4501 tcttgagacc gcgcgaacgc tccccgatgc tgacgccttt ggcgtagccc gtctcgaaga
     4561 agctctgcga ctgcgtgcgg ggggaatcac caaacctgta ctgttactcg aaggcttttt
     4621 tgatgccaga gatctgccga cgatttctgc gcaacatttt cataccgccg tgcataacga
     4681 agaacagctg gctgcgctgg aagaggctag cctggacgag ccggttaccg tctggatgaa
     4741 actcgatacc ggtatgcacc gtctgggcgt aaggccggaa caggctgagg cgttttatca
     4801 tcgcctgacc cagtgcaaaa acgttcgtca gccggtgaat atcgtcagcc attttgcgcg
     4861 cgcggatgaa ccaaaatgtg gcgcaaccga gaaacaactc gctatcttta ataccttttg
     4921 cgaaggcaaa cctggtcaac gttccattgc cgcatcgggt ggcattctgc tgtggccaca
     4981 gtcgcatttt gactgggtgc gcccgggcat cattctttat ggcgtctcgc cgctggaaga
     5041 tcgctccacc ggtgccgatt ttggctgtca gccagtgatg tcactaacct ccagcctgat
     5101 tgccgtgcgt gagcataaag ccggagaacc tgttggttat ggtggaacct gggtaagcga
     5161 acgtgatacc cgtcttggcg tagtcgcgat gggctatggc gatggttatc cgcgcgccgc
     5221 gccgtccggt acgccagtgc tggtgaacgg tcgcgaagta ccgattgtcg ggcgcgtggc
     5281 gatggatatg atctgcgtag acttaggtcc acaagcgcag gacaaagccg gggatccggt
     5341 cattttatgg ggcgaaggtt tgcccgtaga acgtatcgct gaaatgacga aagtaagcgc
     5401 ttacgaactt attacgcgcc tgacttcaag ggtcgcgatg aaatacgtgg atttatcgtt
     5461 ctgtaatatt tgattgtctg tgccggatgc ggcgtgaatg ccttatccgg ccaataaaat
     5521 cctaaaaatt caataagttg atgttctttc atgctcttat aaaggtcgtg cctctggcgg
     5581 atgtacgttt gtcatgagtc tcactctgtt gctaattgcc gttcgctcct gaacatccac
     5641 tcgatcttcg ccttcttccg gtttattgtg ttttaaccac ctgcccgtaa acctggagaa
     5701 ccatcgcgtg tttcaaaaag ttgacgccta cgctggcgac ccgattctta cgcttatgga
     5761 gcgttttaaa gaagaccctc gcagcgacaa agtgaattta agtatcggtc tgtactacaa
     5821 cgaagacgga attattccac aactgcaagc cgtggcggag gcggaagcgc gcctgaatgc
     5881 gcagcctcat ggcgcttcgc tttatttacc gatggaaggg cttaacagct atcgccatgc
     5941 cattgcgccg ctgctgtttg gtgcggacca tccagtactg caacaacagc gcgtagcaac
     6001 cattcaaacc cttggcggct caggggcatt gaaagtgggc gcggatttcc tgaaacgcta
     6061 cttcccggaa tcaggcgtct gggtcagcga tcctacctgg gaaaaccacg tagcaatatt
     6121 cgccggggct ggattcgaag tgagtactta cccctggtat gacgaagcga ctaacggcgt
     6181 gcgctttaat gacctgttgg cgacgctgaa aacattacct gcccgcagta ttgtgttgct
     6241 gcatccatgt tgccacaacc caacgggtgc cgatctcact aatgatcagt gggatgcggt
     6301 gattgaaatt ctcaaatccc gcgagcttat tccattcctc gatattgcct atcaaggatt
     6361 tggtgccggt atggaagagg atgcctacgc tattcgcgcc attgccagcg ctggattacc
     6421 cgctctggtg agcaattcgt tctcgaaaat tttctccctt tacggcgagc gcgtcggcgg
     6481 actttctgtt ctgtgtgaag atgccgaagc cgctggccgc gtactggggc aattgaaagc
     6541 aacagttcgc cgcaactact ccagcccgcc gaattttggt gcgcaggtgg tggctgcagt
     6601 gctgaatgac gaggcattga aagccagctg gctggcggaa gtagaagaga tgcgtactcg
     6661 cattctggca atgcgtcagg aactggtgaa ggtattaagc acagagatgc cagaacgcaa
     6721 tttcgattat ctgcttaatc agcgcggcat gttcagttat accggtttaa gtgccgctca
     6781 ggttgaccga ctacgtgaag aatttggtgt ctatctcatc gccagcggtc gcatgtgtgt
     6841 cgccgggtta aatacggcaa atgtacaacg tgtggcaaag gcgtttgctg cggtgatgta
     6901 atgcaggaaa gcaggctgga gctacccagc ctgcagtgaa attaaactgt cgtcgctttc
     6961 actctttctt tatagatgat ttttttgatg ccatcgttct acgtgagaga taataaacgt
     7021 tgttagt
//
LOCUS       DAZKHF020000225         6984 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2201S31241
            SAMN43267800-rid25106413.denovo.227, whole genome shotgun sequence.
ACCESSION   DAZKHF020000225 DAZKHF020000000
VERSION     DAZKHF020000225.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267800
            Sequence Read Archive: SRR30307263
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6984)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6984)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 6984)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350581.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350581.2
            Genome Coverage       :: 151x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:21:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,652
            CDSs (total)                      :: 5,557
            Genes (coding)                    :: 5,320
            CDSs (with protein)               :: 5,320
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 237
            CDSs (without protein)            :: 237
            Pseudo Genes (ambiguous residues) :: 0 of 237
            Pseudo Genes (frameshifted)       :: 102 of 237
            Pseudo Genes (incomplete)         :: 130 of 237
            Pseudo Genes (internal stop)      :: 52 of 237
            Pseudo Genes (multiple problems)  :: 41 of 237
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6984
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267800-rid25106413.denovo.227"
                     /serotype="O 26:H11"
                     /isolate="2201S31241"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-01"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            <1..909
                     /locus_tag="ACHOSC_RS19065"
                     /old_locus_tag="pgaptmp_003813"
     CDS             <1..909
                     /locus_tag="ACHOSC_RS19065"
                     /old_locus_tag="pgaptmp_003813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001427098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HGU4549662.1"
                     /translation="VMLIVSPSVVASEKAHELYDSIYGGKPAPDVINTLHKMAESGDI
                     DAQSLLGWEYYQPRYDTKPDVQEAIKWFELVAKQGDREAPLALGGIYYDGEQVRVDYA
                     KAYALFNQAAQHGVNLARSRLGIMYANGQYVEVDCKKAKEYLDKGVHIYGGPEDFLAT
                     CRKDMIDRKTVDDTLPVITVTRSGMRDNFLDKGFSCMDSLFATTNKLGEVANLRVTFS
                     IRRPSGKEINQTVGFAPFGLNRLNISFTDYLFGSFTSNSSLILYKPEFERKSCATVRT
                     TIVAATATINGKDVELLKAGAIEQKW"
     gene            complement(965..1507)
                     /locus_tag="ACHOSC_RS19070"
                     /old_locus_tag="pgaptmp_003814"
     CDS             complement(965..1507)
                     /locus_tag="ACHOSC_RS19070"
                     /old_locus_tag="pgaptmp_003814"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000227782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HGU4549663.1"
                     /translation="MVDKHEEITLPIVLSCNYQSDITYPGQKQFDCGNPVIDKFVRAS
                     LKKSVRNSDCAAKALIDRQSGELIGICTFTAYSLEKQRVSGVLQGSQPSEIGVVRLVM
                     LGVARKYQKRGFGQDLLCDFFEHVKIIHQALPIKGVYLDADPAAINFYARLGFVQLSA
                     TPNAFGAVPMFLAIQHILAA"
     gene            complement(1491..1802)
                     /locus_tag="ACHOSC_RS19075"
                     /old_locus_tag="pgaptmp_003815"
     CDS             complement(1491..1802)
                     /locus_tag="ACHOSC_RS19075"
                     /old_locus_tag="pgaptmp_003815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706319.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1778 domain-containing protein"
                     /protein_id="HGU4549664.1"
                     /translation="MPGKTATLPDVDKTLKNARVELKTSPDAKNKLREAAQAVGVDLS
                     AFILSAAMERAESVLDNQRRRELSNQSWELMNQLIAEPAQPTLALKALMKRKNSDGRQ
                     A"
     gene            complement(1987..2877)
                     /gene="cyoE"
                     /locus_tag="ACHOSC_RS19080"
                     /old_locus_tag="pgaptmp_003816"
     CDS             complement(1987..2877)
                     /gene="cyoE"
                     /locus_tag="ACHOSC_RS19080"
                     /old_locus_tag="pgaptmp_003816"
                     /EC_number="2.5.1.141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008495 - protoheme IX farnesyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0048034 - heme O biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heme o synthase"
                     /protein_id="HGU4549665.1"
                     /translation="MMFKQYLQVTKPGIIFGNLISVIGGFLLASKGSIDYPLFIYTLV
                     GVSLVVASGCVFNNYIDRDIDRKMERTKNRVLVKGLISPAVSLVYATLLGIAGFMLLW
                     FGANPLACWLGVMGFVVYVGVYSLYMKRHSVYGTLIGSLSGAAPPVIGYCAVTGEFDS
                     GAAILLAIFSLWQMPHSYAIAIFRFKDYQAANIPVLPVVKGISVAKNHITLYIIAFAV
                     ATLMLSLGGYAGYKYLVVAAAVSVWWLGMALRGYKVADDRIWARKLFGFSIIAITALS
                     VMMSVDFMVPDSHTLLAAVW"
     gene            complement(2889..3218)
                     /locus_tag="ACHOSC_RS19085"
                     /old_locus_tag="pgaptmp_003817"
     CDS             complement(2889..3218)
                     /locus_tag="ACHOSC_RS19085"
                     /old_locus_tag="pgaptmp_003817"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome o ubiquinol oxidase subunit IV"
                     /protein_id="HGU4549666.1"
                     /translation="MSHSTDHSGASHGSVKTYMTGFILSIILTVIPFWMVMTGAASPA
                     VILGTILAMAVVQVLVHLVCFLHMNTKSDEGWNMTAFVFTVLIIAILVVGSIWIMWNL
                     NYNMMMH"
     gene            complement(3218..3832)
                     /locus_tag="ACHOSC_RS19090"
                     /old_locus_tag="pgaptmp_003818"
     CDS             complement(3218..3832)
                     /locus_tag="ACHOSC_RS19090"
                     /old_locus_tag="pgaptmp_003818"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA];
                     GO_process: GO:0022904 - respiratory electron transport
                     chain [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome o ubiquinol oxidase subunit III"
                     /protein_id="HGU4549667.1"
                     /translation="MATDTLTHATAHAHEHGHHDAGGTKIFGFWIYLMSDCILFSILF
                     ATYAVLVNGTAGGPTGKDIFELPFVLVETFLLLFSSITYGMAAIAMYKNNKSQVISWL
                     ALTWLFGAGFIGMEIYEFHHLIVNGMGPDRSGFLSAFFALVGTHGLHVTSGLIWMAVL
                     MVQIARRGLTSTNRTRIMCLSLFWHFLDVVWICVFTVVYLMGAM"
     gene            complement(3822..5813)
                     /gene="cyoB"
                     /locus_tag="ACHOSC_RS19095"
                     /old_locus_tag="pgaptmp_003819"
     CDS             complement(3822..5813)
                     /gene="cyoB"
                     /locus_tag="ACHOSC_RS19095"
                     /old_locus_tag="pgaptmp_003819"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002444673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome o ubiquinol oxidase subunit I"
                     /protein_id="HGU4549668.1"
                     /translation="MFGKLSLDAVPFHEPIVMVTIAGIILGGLALVGLITYFGKWTYL
                     WKEWLTSVDHKRLGIMYIIVAIVMLLRGFADAIMMRSQQALASAGEAGFLPPHHYDQI
                     FTAHGVIMIFFVAMPFVIGLMNLVVPLQIGARDVAFPFLNNLSFWFTVVGVILVNVSL
                     GVGEFAQTGWLAYPPLSGIEYSPGVGVDYWIWSLQLSGIGTTLTGINFFVTILKMRAP
                     GMTMFKMPVFTWASLCANVLIIASFPILTVTVALLTLDRYLGTHFFTNDMGGNMMMYI
                     NLIWAWGHPEVYILILPVFGVFSEIAATFSRKRLFGYTSLVWATVCITVLSFIVWLHH
                     FFTMGAGANVNAFFGITTMIIAIPTGVKIFNWLFTMYQGRIVFHSAMLWTIGFIVTFS
                     VGGMTGVLLAVPGADFVLHNSLFLIAHFHNVIIGGVVFGCFAGMTYWWPKAFGFKLNE
                     TWGKRAFWFWIIGFFVAFMPLYALGFMGMTRRLSQQIDPQFHTMLMIAASGAVLIALG
                     ILCLVIQMYVSIRDRDQNRDLTGDPWGGRTLEWATSSPPPFYNFAVVPHVHERDAFWE
                     MKEKGEAYKKPDHYEEIHMPKNSGAGIVIAAFSTIFGFAMIWHIWWLAIVGFAGMIIT
                     WIVKSFDEDVDYYVPVAEIEKLENQHFDEITKAGLKNGN"
     gene            complement(5835..6782)
                     /gene="cyoA"
                     /locus_tag="ACHOSC_RS19100"
                     /old_locus_tag="pgaptmp_003820"
     CDS             complement(5835..6782)
                     /gene="cyoA"
                     /locus_tag="ACHOSC_RS19100"
                     /old_locus_tag="pgaptmp_003820"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome o ubiquinol oxidase subunit II"
                     /protein_id="HGU4549669.1"
                     /translation="MRLRKYNKSLGWLSLFAGTVLLSGCNSALLDPKGQIGLEQRSLI
                     LTAFGLMLIVVIPAILMAVGFAWKYRASNKDAKYSPNWSHSNKVEAVVWTVPILIIIF
                     LAVLTWKTTHALEPSKPLAHDEKPITIEVVSMDWKWFFIYPEQGIATVNEIAFPANTP
                     VYFKVTSNSVMNSFFIPRLGSQIYAMAGMQTRLHLIANEPGTYDGISASYSGPGFSGM
                     KFKAIATPDRAEFDQWVAKAKQSPNTMSDMAAFEKLAAPSEYNQVEYFSNVKPDLFAD
                     VINKFMAHGKSMDMTQPEGEHSAHEGMEGMDMSHAESAH"
BASE COUNT         1894 a         1753 c         1743 g         1594 t
ORIGIN      
        1 gtaatgctga tagtttcacc aagcgtggtg gccagcgaga aagcgcatga attgtatgac
       61 agcatatacg gtggtaaacc tgctcccgat gttataaata cactgcataa aatggctgaa
      121 tcgggagata tcgacgcgca gagcctgtta ggctgggaat attatcagcc tcgttatgat
      181 accaaacctg atgttcagga agcgattaaa tggttcgagt tagtggctaa acaaggtgac
      241 agagaagctc cgttagcgct ggggggtatc tactacgacg gtgagcaggt gcgggtggat
      301 tatgccaaag cgtatgcact atttaatcag gccgcgcagc acggtgtgaa tttagcaagg
      361 tccaggttgg gcattatgta cgccaatggt cagtatgttg aggtagattg taagaaagcg
      421 aaggaatatc ttgataaagg tgtccacatt tatggtggcc cagaagactt tctggctact
      481 tgtcgaaaag acatgattga cagaaaaacg gttgacgata cgttacccgt gattacggtt
      541 acccgttctg ggatgcgaga taatttttta gataaaggtt tttcctgcat ggatagcctc
      601 ttcgccacca ccaataaatt aggcgaagtg gccaatcttc gcgtgacatt cagcattcgt
      661 cgcccatccg gtaaggagat taaccaaacg gttggcttcg cgcctttcgg tttaaaccgg
      721 ctgaatatta gctttactga ttacctattt ggttcattta ccagtaattc gtctcttatt
      781 ctttataaac cggaatttga gcgaaaatct tgtgcaactg taaggaccac aatcgttgcc
      841 gcaacggcaa ccattaacgg caaggatgtg gagttgctga aagcgggggc aattgaacaa
      901 aagtggtaac gctgctcagc ccccggctga ggcaaaactt gccctggcgg gtggcgataa
      961 taatctaagc cgcgagaata tgctgaatcg ccaaaaacat aggtacagca ccaaaagcat
     1021 ttggtgtcgc tgaaagctga acaaagccga gacgagcata aaaattaatg gcggcagggt
     1081 cagcatcaag ataaacccct ttaattggta atgcctggtg aattattttt acatgttcaa
     1141 aaaaatcaca tagtaggtcc tgaccaaagc cccgcttttg atacttccgt gctaccccca
     1201 acatgactaa tctgacaaca ccaatttctg aaggttgtga accctgaagg acgccagaaa
     1261 cgcgttgttt ttccagcgaa tatgccgtaa aagtacagat gccgatcagt tcaccacttt
     1321 gtctgtcaat aagtgcttta gccgcacagt cgctattacg cacacttttc tttagcgatg
     1381 cgcgtacaaa tttatcgata acagggttac cgcaatcaaa ctgtttttgc ccaggataag
     1441 taatatcaga ctgataatta caggagagga ctatgggcag agtaatctct tcatgcttgt
     1501 cgaccatcgc tgtttttcct tttcattaac gccttgaggg cgagcgtcgg ttgagcaggt
     1561 tcagcgatga gttggttcat cagttcccag ctttgattcg aaagctcacg gcgacgttgg
     1621 ttatcaagca cactttcggc gcgttccata gcagcgctca aaataaaagc gcttaaatct
     1681 accccaacgg cttgcgcagc ttcacgaagt ttgtttttgg cgtcgggact agttttgagt
     1741 tcgacccgcg catttttcag cgttttatcg acatcgggta gagtggctgt ttttccaggc
     1801 attatgacct ctttgattaa ctgaatgaaa tcgtagggag tcgatctcat acataatacg
     1861 gctatcaacc gtatgattaa tttatgttca tctgctcact ctgtcaagac atacggttat
     1921 tagccgtatc tctgatctca gacaataaaa aaggtgccgt agcacctttt taatagagag
     1981 gttttgttac cacacagcag ccagcagcgt atgcgagtcc ggtaccataa aatcaacgga
     2041 catcatcacc gagagggcag tgatggcgat gatagagaag ccgaacagct tgcgcgccca
     2101 gattctgtca tcagcaactt tataaccgcg cagagccata cctaaccacc agacgctaac
     2161 cgccgcggcg accaccagat atttatatcc agcgtaaccg ccaagagaga gcatcagcgt
     2221 ggcaacggca aaggcgatga tatacagcgt gatgtgattc ttcgccaccg aaatgccttt
     2281 taccactggc aataccggaa tgtttgccgc ctggtaatcc ttaaagcgga aaatggcgat
     2341 ggcataggag tgaggcatct gccacaggct gaagatagcc agcaggatcg ctgcgccgct
     2401 atcgaactcg ccggttactg cacagtagcc aatcaccggc ggcgcagcgc cggagagcga
     2461 accaatcaat gtgccgtaga ctgagtggcg tttcatgtac aggctataga cgccgacata
     2521 aaccacaaag cccatcaccc ccagccagca ggccagcgga ttcgcgccaa accacagcag
     2581 cataaagcca gcaataccca gcaaggtggc gtacaccagc gagacagcag gagagatcag
     2641 gcctttaacc agcacccgat tcttcgtcct ttccatcttt ctgtcgatat ccctgtcgat
     2701 gtagttgtta aacacacaac ccgacgccac aaccagtgac accccaacca gcgtgtagat
     2761 aaacagggga taatcaatgc tgccctttga ggccagcagg aatcccccaa tcaccgagat
     2821 caggttgcca aagatgatgc ctggtttcgt tacttgcagg tattgcttaa acatcataac
     2881 cgccgctctt agtgcatcat catgttgtag ttgaggttcc acataatcca gatggagcct
     2941 acaaccagga tagcgatgat tagcacggtg aagacaaacg ccgtcatgtt ccagccttca
     3001 tctgatttgg tattcatgtg caggaagcac accagatgca ccagaacctg taccactgcc
     3061 attgccagga ttgttcccag aattacggcc ggagaggcag cccctgtcat caccatccag
     3121 aacggaatca ccgtcagaat gatcgacagg ataaagcctg tcatgtaggt ttttacgctg
     3181 ccatgggacg cgccgctgtg atcggtagaa tgactcatta catcgccccc atcagataaa
     3241 caacagtgaa cacacagatc caaaccacat ccaggaagtg ccagaacagg ctcaggcaca
     3301 tgatgcgggt acggttagtg ctggtcaggc cgcgacgagc gatttgtacc atcagcaccg
     3361 ccatccagat aagaccggaa gtgacgtgca gaccgtgcgt gccgaccagc gcaaagaacg
     3421 ctgacaggaa gccgctgcga tccggaccca tgccgttaac aatcaggtga tggaattcgt
     3481 agatttccat cccgataaat ccggcaccaa acaaccaggt caacgccagc caggagataa
     3541 cctggctttt gttgtttttg tacatggcga tagccgccat gccgtaggtg atggagctga
     3601 acaacagcaa gaaagtttca accagaacga acggcagttc gaaaatgtcc ttacctgtcg
     3661 ggccgcctgc ggtgccgttc accagaacgg cataggtagc aaacaagatt gagaacagaa
     3721 tgcagtcgct catcaggtag atccaaaatc cgaagatttt ggttccgcct gcatcgtggt
     3781 gcccgtgttc gtgcgcgtgg gcagtcgcgt gcgtcaaagt atcagttgcc atttttcagc
     3841 cctgccttag taatctcatc gaaatgctgg ttttccagtt tttcgatttc tgccaccggc
     3901 acgtagtaat ccacgtcctc gtcgaagctt ttcacgatcc aggtgatgat catgcctgcg
     3961 aagccaacaa tcgccagcca ccagatatgc cagatcatgg cgaaaccgaa gatggtggag
     4021 aaagctgcaa tgacgatacc tgcaccgctg tttttcggca tatgaatttc ttcatagtgg
     4081 tcaggctttt tatacgcttc gcctttctct ttcatttccc agaatgcatc acgctcgtga
     4141 acgtgcggca caacggcaaa gttatagaac ggaggcgggg aagaggttgc ccactccagc
     4201 gtacggccac cccacgggtc gccagtcagg tcacggttct ggtcgcggtc gcgaatagaa
     4261 acgtacatct gaataacgag gcagagaata cccagcgcaa tcagtactgc accgctagct
     4321 gcaatcatca gcatggtgtg gaactgcggg tcaatctgct ggctcaaacg acgggtcatg
     4381 cccatgaagc ccagcgcata cagcggcata aaggcaacga agaagccgat gatccagaac
     4441 cagaacgcgc gtttacccca ggtttcattc agtttgaaac cgaaggcttt aggccaccag
     4501 taggtcatcc ctgcgaagca gccgaagacc acgccgccga tgatcacgtt atggaagtgc
     4561 gcaatcagga acaggctgtt atgcagaacg aagtccgcgc ccggtactgc cagcagcacg
     4621 ccagtcatcc cgcccaccga gaaagtgacg ataaaaccga tggtccacag catcgcagaa
     4681 tggaacacga tgcggccctg atacatggtg aacagccagt tgaagatctt caccccggtc
     4741 gggatggcga taatcattgt ggtgatacca aagaaggcgt ttacgttcgc gcccgcaccc
     4801 atcgtaaaga agtggtgcag ccaaacgatg aacgacagca cggtgataca gacggttgcc
     4861 cataccagcg aggtataacc aaacagacgt ttacgcgaga aggttgccgc aatttcggag
     4921 aacacaccga aaacaggcag gatcaggatg taaacttccg ggtggcccca ggcccaaatc
     4981 aggttgatgt acatcatcat gttgccaccc atatcgttgg taaagaaatg ggtgcccaga
     5041 tagcgatcca gggtcaacaa cgcgacggta accgtcagaa ttgggaagga agcaataatc
     5101 agtacgttcg cgcacagtga tgcccaggta aatactggca tcttgaacat ggtcatgccc
     5161 ggtgcgcgca tcttcagaat ggtaacgaag aagttgatac cggtaagcgt cgtacctata
     5221 ccggatagct ggagactcca tatccagtaa tcgacaccga ctcccggact gtactctatt
     5281 cccgatagcg gtggataggc cagccagccg gtctgcgcaa attcgcccac gccgagagaa
     5341 acgttaacca gaatcacacc aacaacagta aaccagaagc ttaagttgtt gaggaacggg
     5401 aacgcaacgt cacgcgcgcc gatctgcagc ggaaccacca ggttcatcag accgataacg
     5461 aaaggcatcg ccacgaagaa gatcataatc acgccgtgcg cggtgaagat ctgatcgtag
     5521 tggtgaggtg gcaggaagcc cgcttcgccc gccgaggcaa gagcctgctg gctacgcatc
     5581 ataatggcgt cagcaaaacc acgcagcaac atcacaatcg ccacgatgat atacatgata
     5641 ccgaggcgtt tatggtcgac ggaggtcagc cactctttcc acaggtaggt ccacttaccg
     5701 aagtaagtga tcaggccaac gagcgccaga cctcccaaaa taatgccagc gatcgtaacc
     5761 atgacgatag gttcatggaa cgggactgca tcaagtgata attttccgaa catctttatt
     5821 cttcctcaac ccctttaatg ggcggattcc gcgtggctca tgtccatgcc ttccatacct
     5881 tcgtgtgcac tgtgctcacc ttctggctgg gtcatgtcca tgctcttacc gtgagccata
     5941 aacttgttaa tcacatcggc aaacaagtct ggtttcacgt tggagaaata ttccacctgg
     6001 ttgtattcgc taggcgcggc cagtttttcg aacgcagcca tgtcagacat ggtgttcggc
     6061 gactgtttcg cttttgcgac ccattggtcg aattcggcgc gatccggtgt tgcaatagct
     6121 ttgaacttca tgcctgagaa gcccggcccg ctgtagctgg cggagatacc gtcataagta
     6181 ccgggttcgt tggcgatcag atgcaggcga gtctgcatac cggccatggc ataaatctgg
     6241 ctacccagac gcggaatgaa gaaggagttc atcacggagt tggaggtcac tttgaagtac
     6301 accggagtgt tcgccgggaa agcgatttca ttcacggtag caatgccctg ttccgggtag
     6361 atgaagaacc atttccagtc catggaaacc acttcgatgg taatgggctt ctcgtcgtgt
     6421 gccagcggct tgctaggctc aagagcgtga gtggttttcc aggtcagtac cgcaaggaag
     6481 atgatgatta agataggtac cgtccagacc acagcttcca ctttattgga gtgtgaccag
     6541 ttcgggctgt acttagcatc tttattgctc gcacgatact tccaggcgaa accaacagcc
     6601 atcaagattg cgggaataac gacaatcaac atcaggccaa atgccgtcag tatcagtgaa
     6661 cgttgctcca gaccaatctg tcctttggga tctaacagcg cagaattaca gccactgagc
     6721 aatacagtgc ctgcaaataa tgacaaccat cccaaacttt tattgtattt cctgagtctc
     6781 atttaacgac ctcaattcca cgggatctgg tggcgtttaa agtgtgtggg cattttacgg
     6841 gaaggttaca ttactgtaaa catgattaaa ttcctgttac ttggatttgg ctgtcaggtc
     6901 acatatgttg caaaacatat caagttgatt ttaagaagct gttgcaaaag ggcgttataa
     6961 cgaaagggga gtgcagaagg agtt
//
LOCUS       DAZKHF020000226         6974 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2201S31241
            SAMN43267800-rid25106413.denovo.228, whole genome shotgun sequence.
ACCESSION   DAZKHF020000226 DAZKHF020000000
VERSION     DAZKHF020000226.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267800
            Sequence Read Archive: SRR30307263
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6974)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6974)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 6974)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350581.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350581.2
            Genome Coverage       :: 151x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:21:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,652
            CDSs (total)                      :: 5,557
            Genes (coding)                    :: 5,320
            CDSs (with protein)               :: 5,320
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 237
            CDSs (without protein)            :: 237
            Pseudo Genes (ambiguous residues) :: 0 of 237
            Pseudo Genes (frameshifted)       :: 102 of 237
            Pseudo Genes (incomplete)         :: 130 of 237
            Pseudo Genes (internal stop)      :: 52 of 237
            Pseudo Genes (multiple problems)  :: 41 of 237
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6974
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267800-rid25106413.denovo.228"
                     /serotype="O 26:H11"
                     /isolate="2201S31241"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-01"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            120..731
                     /gene="ruvA"
                     /locus_tag="ACHOSC_RS19105"
                     /old_locus_tag="pgaptmp_003821"
     CDS             120..731
                     /gene="ruvA"
                     /locus_tag="ACHOSC_RS19105"
                     /old_locus_tag="pgaptmp_003821"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012131997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009379 - Holliday junction helicase
                     complex [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0009378 - four-way junction helicase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration protein RuvA"
                     /protein_id="HGU4549670.1"
                     /translation="MIGRLRGIIIEKQPPLVLIEVGGVGYEVHMPMTCFYELPEAGQE
                     AIVFTHFVVREDAQLLYGFNNKQERTLFKELIKTNGVGPKLALAILSGMSAQQFVNAV
                     EREEVGALVKLPGIGKKTAERLIVEMKDRFKGLHGDLFTPAADLVLTSPASPTTDDAE
                     QEAVAALVALGYKPQEASRMVSKIARPDASSETLIREALRAAL"
     gene            740..1750
                     /gene="ruvB"
                     /locus_tag="ACHOSC_RS19110"
                     /old_locus_tag="pgaptmp_003822"
     CDS             740..1750
                     /gene="ruvB"
                     /locus_tag="ACHOSC_RS19110"
                     /old_locus_tag="pgaptmp_003822"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0009378 - four-way junction helicase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration DNA helicase
                     RuvB"
                     /protein_id="HGU4549671.1"
                     /translation="MIEADRLISAGTTLPEDVADRAIRPKLLEEYVGQPQVRSQMEIF
                     IKAAKLRGDALDHLLIFGPPGLGKTTLANIVANEMGVNLRTTSGPVLEKAGDLAAMLT
                     NLEPHDVLFIDEIHRLSPVVEEVLYPAMEDYQLDIMIGEGPAARSIKIDLPPFTLIGA
                     TTRAGSLTSPLRDRFGIVQRLEFYQVPDLQYIVSRSARFMGLEMSDDGALEVARRARG
                     TPRIANRLLRRVRDFAEVKHDGTISADIAAQALDMLNVDAEGFDYMDRKLLLAVIDKF
                     FGGPVGLDNLAAAIGEERETIEDVLEPYLIQQGFLQRTPRGRMATTRAWNHFGITPPE
                     MP"
     gene            complement(1897..2682)
                     /gene="znuB"
                     /locus_tag="ACHOSC_RS19115"
                     /old_locus_tag="pgaptmp_003823"
     CDS             complement(1897..2682)
                     /gene="znuB"
                     /locus_tag="ACHOSC_RS19115"
                     /old_locus_tag="pgaptmp_003823"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter permease subunit ZnuB"
                     /protein_id="HGU4549672.1"
                     /translation="MIELLFPGWLAGIMLACAAGPLGSFVVWRRMSYFGDTLAHASLL
                     GVAFGLLLDVNPFYAVIAVTLLLAGGLVWLEKRPQLAIDTLLGIMAHSALSLGLVVVS
                     LMSNIRVDLMAYLFGDLLAVTPEDLIAIAIGVVIVVAILFWQWRNLLSMTISPDLAFV
                     DGVKLQRVKLLLMLVTALTIGVAMKFVGALIITSLLIIPAATARRFARTPEQMAGVAV
                     LVGMVAVTGGLTFSAFYDTPAGPSVVLCAALLFILSMMKKQAS"
     gene            complement(2679..3434)
                     /gene="znuC"
                     /locus_tag="ACHOSC_RS19120"
                     /old_locus_tag="pgaptmp_003824"
     CDS             complement(2679..3434)
                     /gene="znuC"
                     /locus_tag="ACHOSC_RS19120"
                     /old_locus_tag="pgaptmp_003824"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015633 - ABC-type zinc transporter
                     activity [Evidence IEA];
                     GO_process: GO:0006829 - zinc ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter ATP-binding protein ZnuC"
                     /protein_id="HGU4549673.1"
                     /translation="MTSLVSLENVSVSFGQRRVLSDVSLELKPGKILTLLGPNGAGKS
                     TLVRVVLGLVTPDEGVIKRNGKLRIGYVPQKLYLDTTLPLTVNRFLRLRPGTHKEDIL
                     PALKRVQAGHLINAPMQKLSGGETQRVLLARALLNRPQLLVLDEPTQGVDVNGQVALY
                     DLIDQLRRELDCGVLMVSHDLHLVMAKTDEVLCLNHHICCSGTPEVVSLHPEFISMFG
                     PRGAEQLGIYRHHHNHRHDLQGRIVLRRGNDRS"
     gene            3513..4445
                     /gene="znuA"
                     /locus_tag="ACHOSC_RS19125"
                     /old_locus_tag="pgaptmp_003825"
     CDS             3513..4445
                     /gene="znuA"
                     /locus_tag="ACHOSC_RS19125"
                     /old_locus_tag="pgaptmp_003825"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416371.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006829 - zinc ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter substrate-binding protein
                     ZnuA"
                     /protein_id="HGU4549674.1"
                     /translation="MLHKKTLLFAALSAALWGGATQAADAAVVASLKPVGFIASAIAD
                     GVTETEVLLPDGASEHDYSLRPSDVKRLQNADLVVWVGPEMEAFMQKPVSKLPGAKQV
                     TIAQLEDVKPLLMKSIHGDDDDHDHAEKSDEDHHHGDFNMHLWLSPEIARATAVAIHG
                     KLVELMPQSRAKLDANLKDFEAQLASTETQVGNELAPLKGKGYFVFHDAYGYFEKQFG
                     LTPLGHFTVNPEIQPGAQRLHEIRTQLVEQKATCVFAEPQFRPAVVESVARGTSVRMG
                     TLDPLGTNIKLGKTSYSEFLSQLANQYASCLKGD"
     gene            4461..5783
                     /gene="mepM"
                     /locus_tag="ACHOSC_RS19130"
                     /old_locus_tag="pgaptmp_003826"
     CDS             4461..5783
                     /gene="mepM"
                     /locus_tag="ACHOSC_RS19130"
                     /old_locus_tag="pgaptmp_003826"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="murein DD-endopeptidase MepM"
                     /protein_id="HGU4549675.1"
                     /translation="MQQIARSVALAFNNLPRPHRVMLGSLTVLTLAVAVWRPYVYHRD
                     ATPIVKTIELEQNEIRSLLPEASEPIDQATQEDEAIPQDELDDKIAGEAGVHEYVVST
                     GDTLSSILNQYGIDMGDITQLAAADKELRNLKIGQQLSWTLTADGELQRLTWEVSRRE
                     TRTYDRTAANGFKMTSEMQQGEWVNNLLKGTVGGSFVASARNAGLTSAEVSAVIKAMQ
                     WQMDFRKLKKGDEFAVLMSREMLDGKREQSQLLGVRLRSEGKDYYAIRAEDGKFYDRN
                     GTGLAKGFLRFPTAKQFRISSNFNPRRTNPVTGRVAPHRGVDFAMPQGTPVLSVGDGE
                     VVVAKRSGAAGYYVAIRHGRSYTTRYMHLRKILVKPGQKVKRGDRIALSGNTGRSTGP
                     HLHYEVWINQQVVNPLTAKLPRTEGLTGSDRREFLAQAKEIVPQLRFD"
     gene            5903..6874
                     /gene="lpxM"
                     /locus_tag="ACHOSC_RS19135"
                     /old_locus_tag="pgaptmp_003827"
                     /gene_synonym="msbB"
     CDS             5903..6874
                     /gene="lpxM"
                     /locus_tag="ACHOSC_RS19135"
                     /old_locus_tag="pgaptmp_003827"
                     /gene_synonym="msbB"
                     /EC_number="2.3.1.243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416369.1"
                     /note="LpxM is lauroyl-Kdo(2)-lipid IV(A)
                     myristoyltransferase, an enzyme characterized in
                     Escherichia coli and involved in biosynthesis of the form
                     of lipid A found in that species and some closely related
                     species; Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009276 - Gram-negative-bacterium-type
                     cell wall [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lauroyl-Kdo(2)-lipid IV(A) myristoyltransferase"
                     /protein_id="HGU4549676.1"
                     /translation="METKKNNSEYIPEFDKSFRHPRYWGAWLGVAAMAGIALTPPKFR
                     DPILARLGRFAGRLGKSSRRRALINLSLCFPERSEAEREAIVDEMFATAPQAMAMMAE
                     LAIRGPEKIQPRVDWQGLEIIEEMRRNNEKVIFLVPHGWAVDIPAMLMASQGQKMAAM
                     FHNQGNPVFDYVWNTVRRRFGGRLHARNDGIKPFIQSVRQGYWGYYLPDQDHGPEHSE
                     FVDFFATYKATLPAIGRLMKVCRARVVPLFPIYDGKTHRLTIQVRPPMDDLLEADDHT
                     IARRMNEEVEIFVGPRPEQYTWILKLLKTRKPGEIQPYKRKDLYPIK"
BASE COUNT         1809 a         1717 c         1822 g         1626 t
ORIGIN      
        1 tgcgccattt ttcagatcgt tgggacgcct cgcaagtttt cttcatcctt cgctggatat
       61 ctatccagca tttttttatc atacagcatt atctttgatt cattacgcag gagcgtcatg
      121 tgataggcag actcagaggc atcatcattg aaaaacaacc cccgctggtg ttaattgaag
      181 tgggcggcgt aggctatgaa gtgcatatgc cgatgacctg tttttatgaa ctccctgaag
      241 cgggtcagga agcgatcgtt ttcacccact ttgtggtgcg tgaagacgcg caactgctgt
      301 acggttttaa caataaacaa gagcgcacat tgttcaaaga gttgatcaaa accaacggcg
      361 tcggcccgaa gttggcgctg gcgatcctct ccggaatgtc agcgcagcag ttcgttaatg
      421 ccgttgagcg tgaagaagtg ggggcactgg tgaaactgcc gggtattggc aaaaaaaccg
      481 ccgaacgctt gattgttgaa atgaaagacc gatttaaagg tttgcatggc gatctcttta
      541 cgccagccgc cgacctggta ctcacgtcac ctgccagccc gacgaccgac gatgctgaac
      601 aggaagcggt tgccgcgctg gtggcgctgg gctataaacc acaagaagca agccgcatgg
      661 tgagcaaaat cgctcgccct gacgccagca gtgaaacatt aattcgcgaa gccctacgcg
      721 ccgcgttatg aggtaaagga tgattgaagc agaccgtctg atttctgccg gtaccacttt
      781 gccggaagat gtggcagatc gcgccattcg ccctaaatta ctggaagagt atgttggtca
      841 gccgcaggtt cgttcacaga tggagatttt catcaaagca gcgaaactgc gcggcgatgc
      901 cctcgatcat ctgttgattt tcggtcctcc ggggttgggt aaaactacgc ttgccaacat
      961 tgtcgccaat gaaatgggcg ttaatttacg cacgacttct ggtccggtgc tggaaaaggc
     1021 gggcgatctg gcagcgatgc tcactaacct tgaaccgcat gacgtgttgt ttattgatga
     1081 gatccaccgt ctttcgccag tggtggaaga ggtgttgtat ccggcaatgg aagactacca
     1141 actggatatc atgattggtg aaggtccggc ggcacgctcc attaaaattg atttgccgcc
     1201 gtttaccctg attggtgcaa ccacgcgcgc aggttcgctg acatcaccgt tgcgcgatcg
     1261 ttttggtatt gtgcagcgtc tggagtttta tcaggtgccg gatctgcaat atatcgtcag
     1321 tcgcagcgca cgctttatgg ggcttgagat gagtgatgac ggcgcgctgg aagttgctcg
     1381 tcgagctcgc ggtacgccgc gcattgccaa ccgtctgctg cgtcgagtgc gtgatttcgc
     1441 cgaagtgaag cacgatggca ccatctcggc agatatcgct gctcaggcgt tggatatgtt
     1501 gaatgtcgat gctgaaggtt tcgattatat ggaccgcaag ttattgctgg cggtaatcga
     1561 taagttcttt ggtgggccgg taggtctgga taaccttgcg gcagccattg gcgaagaacg
     1621 tgaaaccatt gaggatgtgc tggaacctta tttgattcag caaggctttt tgcagcgtac
     1681 accgcgtggg cgtatggcga cgacgcgggc gtggaatcac tttggcataa cgccgccaga
     1741 aatgccgtaa gtcggattgg tcgctcgcgc cgcatccgac actggcagtg tgtctgatgc
     1801 gacactgttc gcgtcttatc aggccagcca gagatagcga accgtaggtc ggataaggcg
     1861 ctcgcgccgc atccgacaaa tgtgttcagc gatagattag ctggcctgct ttttcatcat
     1921 actgagaata aataacagtg ccgcacatag caccaccgac ggacccgccg gcgtatcgta
     1981 aaacgcggaa aaggttaaac cgccagttac tgccaccatc cccaccaaaa cagcgacacc
     2041 agccatctgt tccggcgtac gggcaaagcg acgcgcagta gctgcaggaa taatcagcag
     2101 tgaagtaata atcaacgcgc caacaaattt catcgctaca ccaatcgtca atgccgtcac
     2161 cagcatcaac aacaatttca cgcgctgtaa tttcacacca tcaacaaacg ccagatccgg
     2221 gctaatcgtc atcgacagca aattgcgcca ttgccagaac aaaatagcca ccacgatgac
     2281 cacgccaatc gcaatagcga tgagatcttc tggcgtcact gccagcaaat caccgaacag
     2341 gtaagccatc aaatcaacac gaatgttaga catcagacta acgaccacca ggcccagcga
     2401 cagggcactg tgcgccataa tccctaataa cgtgtcaatc gccagctgtg gacgcttctc
     2461 cagccatacc agaccgcccg ccaggagcag cgtaacggca attaccgcat agaatggatt
     2521 cacgtccagc aacaaaccaa acgcgacgcc aagtaatgag gcatgagcca gcgtatcacc
     2581 gaaataagac atacgacgcc agactacaaa cgaacccagc ggacccgcgg cacaggcgag
     2641 catgatcccg gctaaccaac cgggaaataa taattcaatc atgagcgatc atttccccga
     2701 cgcaaaacaa ttcgtccctg taaatcgtga cgatgattat gatgatggcg atagataccc
     2761 agttgttcag caccacgagg accaaacata gaaataaact ccggatgtag ggaaacaact
     2821 tccggtgtgc cggaacaaca aatgtggtga ttcagacaca gcacttcatc ggtttttgcc
     2881 attaccagat gcagatcgtg agagaccatt aaaacgccac aatccagttc gcgacgcaac
     2941 tggtcaataa ggtcatataa cgccacctgg ccatttacat ccacgccttg agtgggttca
     3001 tccagcacta ataattgcgg tcgatttaac aatgctcgcg ctaacagtac acgctgtgtt
     3061 tcgccaccag agagcttttg catcggtgcg ttaatcagat gcccggcctg gacacgtttc
     3121 agtgcaggca aaatatcttc tttatgtgtg ccagggcgta agcgtaaaaa acggtttacg
     3181 gtcagtggca acgtggtgtc gagatacagc ttctgcggta catagccgat gcgtagtttt
     3241 ccgttgcgct tgataacccc ttcatcgggt gttaccagcc cgagcactac ccgtaccagt
     3301 gtcgacttac ctgcgccatt tggcccaagt aaagtcaaaa tttttccagg tttaagttcc
     3361 agcgacacat cagagaggac gcggcgttgg ccaaaagaaa ccgagacatt ttccagggaa
     3421 accagacttg tcatgttaat tttagtcttg cagtagtcat gaaatgttat aatatcacac
     3481 ttctcatatt cattacgatg attggtcgca ttatgttaca taaaaaaacg cttcttttcg
     3541 cagcattatc cgccgctctc tggggaggtg caacacaggc cgcagatgcc gccgttgtcg
     3601 cttcgcttaa acccgttggg ttcatcgctt ctgccattgc tgatggtgta acagaaacgg
     3661 aggttttact gcctgacggc gcttcagaac atgattattc actgcgtcca tcggatgtaa
     3721 aacgcttaca gaacgcggac ttagtcgttt gggttggccc ggagatggaa gcgtttatgc
     3781 aaaaaccggt aagcaaatta ccaggagcga agcaggtaac gattgcgcag cttgaagatg
     3841 tgaaaccgct actgatgaaa agtattcacg gcgatgatga tgatcacgac cacgcggaaa
     3901 aaagtgacga agatcaccat cacggcgatt tcaacatgca tctttggctt tccccagaga
     3961 tagcgcgggc tacagcggtt gcaatccatg gaaaattagt ggaacttatg ccgcaaagtc
     4021 gagccaaact tgacgccaac ctgaaggatt ttgaggcaca attagcctca accgaaacgc
     4081 aggttggtaa cgagctcgcg ccgctcaagg ggaaaggtta tttcgttttt cacgatgctt
     4141 acggctattt cgaaaaacag tttggactga caccgcttgg tcattttacc gttaaccctg
     4201 agattcaacc tggcgcgcag cgtttacatg aaataagaac acagttggtt gagcaaaaag
     4261 caacctgcgt ttttgctgag ccacagttca ggccagcggt cgttgagagc gtcgcacgag
     4321 ggacatccgt tcgtatggga acgttggatc ccctggggac gaatatcaaa ctgggtaaaa
     4381 caagctattc agaattcctg agtcaattag ccaaccagta tgcgagctgc ctgaaaggag
     4441 attaatgagg aagtgattac gtgcaacaga tagcccgctc tgtcgccctg gcgtttaata
     4501 atttaccgcg accacaccgc gttatgttgg ggtcgctcac cgttcttact ctggccgtcg
     4561 ctgtctggcg gccttatgtt tatcaccgtg atgccacgcc aattgtcaaa accattgagc
     4621 tggaacagaa cgaaattcgt tcgctcttac ctgaagccag tgagccgatt gatcaagcta
     4681 cacaagaaga tgaagccatt ccccaggacg aactggatga caaaatcgcc ggtgaagcgg
     4741 gcgtgcatga atatgttgtt tccactggcg atacgctaag cagcattctc aatcagtatg
     4801 gtattgatat gggtgatatc acccaactgg ctgcggccga caaagaattg cgtaacctga
     4861 aaatcggtca acaactctcc tggacattaa ccgcggacgg cgaactgcag cgcctcacct
     4921 gggaagtgtc tcgtcgtgaa acccgaacct atgaccgtac tgccgctaac ggttttaaaa
     4981 tgaccagcga aatgcagcaa ggagagtggg ttaacaatct gctgaaaggt accgtcgggg
     5041 ggagctttgt tgccagcgcc agaaacgccg gtttaaccag cgccgaagtg agcgcagtga
     5101 ttaaagccat gcagtggcaa atggatttcc gcaaactgaa aaaaggcgat gaatttgcgg
     5161 tgttaatgtc tcgagaaatg cttgatggta aacgtgagca aagccagctg ctgggcgtac
     5221 gtttgcgttc agaaggtaaa gattattacg ctatccgcgc tgaagatggc aaattctacg
     5281 atcgtaacgg tactggtctg gcgaaaggat tcttgcgatt cccgacggcg aaacagttcc
     5341 gtatctcgtc taactttaac ccgcgtcgaa ctaatccggt gacaggtcgc gttgcaccac
     5401 acagaggtgt tgatttcgcc atgccacaag gtacgccagt gctttcagtg ggtgacggtg
     5461 aagtggtggt tgccaaacgc agtggcgcag caggttatta tgtggctatt cgtcatggtc
     5521 gcagctacac cacgcgttat atgcacttgc gcaagattct ggtgaaaccg ggacagaagg
     5581 tgaaacgtgg cgaccgtatc gcgctttccg gtaataccgg acgttcaact gggccgcatc
     5641 tgcactatga agtatggata aaccagcagg tcgtaaaccc gctgacggca aaactgccgc
     5701 gtactgaagg gctgaccggc tccgatcgtc gcgaattcct ggcgcaggcc aaagagattg
     5761 tgccgcagct acggtttgat taattaacat ccattcgcag ccggtacgca gtcagtaccg
     5821 gcttttttta tttggtgcgg ggcaagttgc gccgctacac tatcaccaga ttgatttttg
     5881 ccttatccga aactggaaaa gcatggaaac gaaaaaaaat aatagcgaat acattcctga
     5941 gtttgataaa tcctttcgcc acccgcgcta ctggggagca tggctgggtg tagcagcgat
     6001 ggcgggtatc gctttaacgc cgccaaagtt ccgtgatccc attctggcac ggctgggacg
     6061 ttttgccgga cgactgggaa aaagctcacg ccgtcgtgcg ttaatcaatc tgtcgctctg
     6121 ctttccagaa cgtagtgaag ctgaacgcga agcgattgtt gatgagatgt ttgccaccgc
     6181 gccgcaagcg atggcaatga tggctgagtt ggcaatacgc ggaccggaga aaattcagcc
     6241 gcgcgttgac tggcaagggc tggagatcat cgaagagatg cggcgtaata acgagaaagt
     6301 tatctttctg gtgccgcacg gttgggccgt cgatattcct gccatgctga tggcctcgca
     6361 agggcagaaa atggcagcga tgttccataa tcagggcaac ccggtttttg attatgtctg
     6421 gaacacggtg cgtcgtcgct ttggcggtcg tctgcatgcg agaaatgacg gtattaaacc
     6481 attcatccag tcggtacgtc aggggtactg gggatattat ttacccgatc aggatcatgg
     6541 cccagagcac agcgaatttg tggatttctt tgccacctat aaagcgacgt tgcccgcgat
     6601 tggtcgtttg atgaaagtgt gccgtgcgcg cgttgtaccg ctgtttccga tttatgatgg
     6661 caagacgcat cgtctgacga ttcaggtgcg cccaccgatg gatgatctgt tagaggcgga
     6721 tgatcatacg attgcgcggc ggatgaatga agaagtcgag atttttgttg gtccgcgacc
     6781 agaacaatac acctggatac taaaattgct gaaaactcgc aaaccgggcg aaatccagcc
     6841 gtataagcgc aaagatcttt atcccatcaa ataaaaaaca gcctctcaaa ttattgagag
     6901 gctgtacttg ataaaaaatc tcaatgagtg ttctcaccat tttgagtttt tagattagta
     6961 cgctattgag ttaa
//
LOCUS       DAZKHF020000227         6967 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2201S31241
            SAMN43267800-rid25106413.denovo.229, whole genome shotgun sequence.
ACCESSION   DAZKHF020000227 DAZKHF020000000
VERSION     DAZKHF020000227.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267800
            Sequence Read Archive: SRR30307263
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6967)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6967)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 6967)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350581.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350581.2
            Genome Coverage       :: 151x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:21:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,652
            CDSs (total)                      :: 5,557
            Genes (coding)                    :: 5,320
            CDSs (with protein)               :: 5,320
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 237
            CDSs (without protein)            :: 237
            Pseudo Genes (ambiguous residues) :: 0 of 237
            Pseudo Genes (frameshifted)       :: 102 of 237
            Pseudo Genes (incomplete)         :: 130 of 237
            Pseudo Genes (internal stop)      :: 52 of 237
            Pseudo Genes (multiple problems)  :: 41 of 237
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6967
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267800-rid25106413.denovo.229"
                     /serotype="O 26:H11"
                     /isolate="2201S31241"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-01"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            513..1502
                     /gene="araF"
                     /locus_tag="ACHOSC_RS19140"
                     /old_locus_tag="pgaptmp_003828"
     CDS             513..1502
                     /gene="araF"
                     /locus_tag="ACHOSC_RS19140"
                     /old_locus_tag="pgaptmp_003828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arabinose ABC transporter substrate-binding
                     protein AraF"
                     /protein_id="HGU4549677.1"
                     /translation="MHKFTKALAAIGLAAVMSQSAMAENLKLGFLVKQPEEPWFQTEW
                     KFADKAGKDLGFEVIKIAVPDGEKTLNAIDSLAASGAKGFVICTPDPKLGSAIVAKAR
                     GYDMKVIAVDDQFVNAKGKPMDTVPLVMMAATKIGERQGQELYKEMQKRGWDVKESAV
                     MAITANELDTARRRTTGSMDALKAAGFPEKQIYQVPTKSNDIPGAFDAANSMLVQHPE
                     VKHWLIVGMNDSTVLGGVRATEGQGFKAADIIGIGINGVDAVSELSKAQATGFYGSLL
                     PSPDVHGYKSSEMLYNWVAKDVEPPKFTEVTDVVLITRDNFKEELEKKGLGGK"
     gene            1572..3086
                     /gene="araG"
                     /locus_tag="ACHOSC_RS19145"
                     /old_locus_tag="pgaptmp_003829"
     CDS             1572..3086
                     /gene="araG"
                     /locus_tag="ACHOSC_RS19145"
                     /old_locus_tag="pgaptmp_003829"
                     /EC_number="7.5.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005127704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arabinose ABC transporter ATP-binding protein
                     AraG"
                     /protein_id="HGU4549678.1"
                     /translation="MQQSTPYLSFRGIGKTFPGVKALTDISFDCYAGQVHALMGENGA
                     GKSTLLKILSGNYAPTTGSVVINGQEMSFSDTTAALNAGVAIIYQELHLVPEMTVAEN
                     IYLGQLPHKGGIVNRSLLNYEAGLQLKHLGMDIDPDTPLKYLSIGQWQMVEIAKALAR
                     NAKIIAFDEPTSSLSAREIDNLFRVIRELRKEGRVILYVSHRMEEIFALSDAITVFKD
                     GRYVKTFTDMQQVDHDALVQAMVGRDIGDIYGWQPRSYGEERLRLDAVKAPGVRTPIS
                     LAVRSGEIVGLFGLVGAGRSELMKGLFGGTQITAGQVYIDQQPIDIRKPSHAIAAGMM
                     LCPEDRKAEGIIPVHSVRDNINISARRKHVLGGCVINNGWEENNADHHIRSLNIKTPG
                     AEQLIMNLSGGNQQKAILGRWLSEEMKVILLDEPTRGIDVGAKHEIYNVIYALAAQGV
                     AVLFASSDLPEVLGVADRIVVMREGEIAGELLHEQADERQALSLAMPKVSQAVA"
     gene            3101..4087
                     /gene="araH"
                     /locus_tag="ACHOSC_RS19150"
                     /old_locus_tag="pgaptmp_003830"
     CDS             3101..4087
                     /gene="araH"
                     /locus_tag="ACHOSC_RS19150"
                     /old_locus_tag="pgaptmp_003830"
                     /EC_number="7.5.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026162.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arabinose ABC transporter permease AraH"
                     /protein_id="HGU4549679.1"
                     /translation="MSSVSTSGSGAPKSSFSFGRIWDQYGMLVVFAVLFIACAIFVPN
                     FATFINMKGLGLAISMSGMVACGMLFCLASGDFDLSVASVIACAGVTTAVVINLTESL
                     WIGVAAGLLLGILCGLVNGFVIAKLKINALITTLATMQIVRGLAYIISDGKAVGIEDE
                     SFFALGYANWFGLPAPIWLTVACLIIFGLLLNKTTFGRNTLAIGGNEEAARLAGVPVV
                     RTKIIIFVLSGLVSAIAGIILASRMTSGQPMTSIGYELIVISACVLGGVSLKGGIGKI
                     SYVVAGILILGTVENAMNLLNISPFAQYVVRGLILLAAVIFDRYKQKAKRTV"
     gene            4254..5054
                     /gene="otsB"
                     /locus_tag="ACHOSC_RS19155"
                     /old_locus_tag="pgaptmp_003831"
     CDS             4254..5054
                     /gene="otsB"
                     /locus_tag="ACHOSC_RS19155"
                     /old_locus_tag="pgaptmp_003831"
                     /EC_number="3.1.3.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004805 - trehalose-phosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0005992 - trehalose biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trehalose-phosphatase"
                     /protein_id="HGU4549680.1"
                     /translation="MTEPLTETPELSAKYAWFFDLDGTLAEIKPHPDQVVVPDNILQG
                     LQLLATASDGALALISGRSMVELDALAKPYRFPLAGVHGAERRDINGKTHIVHLPDAI
                     ARDISVQLHTVIAQYPGAELEAKGMAFALHYRQAPQHEDALMTLAQRITQIWPQMALQ
                     QGKCVVEIKPRGTSKGEAIAAFMQEAPFIGRTPVFLGDDLTDESGFAVVNRLGGMSVK
                     IGTGATQASWRLAGVPDVWSWLEMITTALQQKRENNRSDDYESFSRSI"
     gene            5029..6453
                     /gene="otsA"
                     /locus_tag="ACHOSC_RS19160"
                     /old_locus_tag="pgaptmp_003832"
     CDS             5029..6453
                     /gene="otsA"
                     /locus_tag="ACHOSC_RS19160"
                     /old_locus_tag="pgaptmp_003832"
                     /EC_number="2.4.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003825 - alpha,alpha-trehalose-phosphate
                     synthase (UDP-forming) activity [Evidence IEA];
                     GO_process: GO:0005992 - trehalose biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha,alpha-trehalose-phosphate synthase"
                     /protein_id="HGU4549681.1"
                     /translation="MSRLVVVSNRIAPPDEHAASAGGLAVGILGALKAAGGLWFGWSG
                     ETGNEDQPLKKVKKGNITWASFNLSEQDLDEYYNQFSNAVLWPAFHYRLDLVQFQRPA
                     WDGYLRVNALLADKLLPLLQDDDIIWIHDYHLLPFAHELRKRGVNNRIGFFLHIPFPT
                     PEIFNALPTYDTLLEQLCDYDLLGFQTENDRLAFLDCLSNLTRVTTRSAKSHTAWGKA
                     FRTEVYPIGIEPKEIAKQAAGPLPPKLAQLKAELKNVQNIFSVERLDYSKGLPERFLA
                     YEALLEKYPQHHGKIRYTQIAPTSRGDVQAYQDIRHQLENEAGRINGKYGQLGWTPLY
                     YLNQHFDRKLLMKIFRYSDVGLVTPLRDGMNLVAKEYVAAQDPANPGVLVLSQFAGAA
                     NELTSALIVNPYDRDEVAAALDRALTMSLAERISRHAEMLDVIVKNDINHWQECFISD
                     LKQIVPRSAESQQRDKVATFPKLA"
     gene            complement(6460..6888)
                     /gene="uspC"
                     /locus_tag="ACHOSC_RS19165"
                     /old_locus_tag="pgaptmp_003833"
     CDS             complement(6460..6888)
                     /gene="uspC"
                     /locus_tag="ACHOSC_RS19165"
                     /old_locus_tag="pgaptmp_003833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein UspC"
                     /protein_id="HGU4549682.1"
                     /translation="MSYSNILVAVAVTPESQQLLAKAVSIARPVKGHISLITLASDPE
                     MYNQLAAPMLEDLRSVMQEETQSFLDKLIQDAGYPVDKTFIAYGELSEHILEVCHKHH
                     FDLVICGNHNHSFFSRASCSAKRVIASSEVDVLLVPLTGD"
BASE COUNT         1753 a         1628 c         1815 g         1771 t
ORIGIN      
        1 tgatgaagtg atgattttat aaaacgtttt ttcatttttg cgagatggct ctcattatac
       61 gtgttctgtt aattaatgag tacagtgata attaatggcg aaatgaatga cgtgcatttt
      121 ccacatcttt gagttgcggt tattacacca tttcaaaaac tcaacgccag gtaatgcggc
      181 ctattgactg gttaaaaaga agacatcccg catgggtacc aaagacaaca aggatttcca
      241 ggctaatctt atggattaat ctgctgtgca ttcgacaatt tgtctgacaa attggctttc
      301 ccttatgtct tttcccgcta aatttatgca cgttctcact gtaattctgc gatgtgatat
      361 tgctctccta tggagaatta atttctcgct aaaactatgt caacacagtc acttatcttt
      421 tagttaaaag gtaatgcttt gttttccgat taatttaacg aatgtcattc gtttttgccc
      481 tacacaaaac gacactaaag ctggagagaa ccatgcacaa atttactaaa gccctggcag
      541 ccattggtct ggcagccgtt atgtcacaat ccgctatggc ggagaacctg aagctcggtt
      601 ttctggtgaa gcaaccggaa gagccgtggt tccagaccga atggaagttt gccgataaag
      661 ccgggaagga tttagggttt gaggttatta agattgccgt gccggatggc gaaaaaacat
      721 tgaacgcgat cgacagcctg gctgccagtg gcgcaaaagg tttcgttatt tgtactccgg
      781 accccaaact cggctctgcc atcgtcgcga aagcgcgtgg ctacgatatg aaagtcattg
      841 ccgtggatga ccagtttgtt aacgccaaag gtaagccaat ggataccgtt ccgctggtga
      901 tgatggcggc gactaaaatt ggcgaacgtc agggccagga actgtataaa gagatgcaga
      961 aacgtggctg ggatgtcaaa gaaagcgcgg tgatggcgat taccgccaac gaactggata
     1021 ccgcccgccg ccgtactacg ggatctatgg atgcgctgaa agcggccgga ttcccggaaa
     1081 aacaaattta tcaggtacct accaaatcta acgacatccc gggggcattt gacgctgcca
     1141 actcaatgct ggttcaacat ccggaagtta aacattggct gatcgtcggt atgaacgaca
     1201 gcaccgtgct gggcggcgta cgcgcgacgg aaggtcaggg ctttaaagcg gccgatatca
     1261 tcggcattgg cattaacggt gtggatgcgg tgagcgaact gtctaaagca caggcaaccg
     1321 gcttctacgg ttccctgctg ccaagcccgg acgtacatgg ctataaatcc agcgaaatgc
     1381 tttacaactg ggtagcaaaa gacgttgaac cgccaaaatt taccgaagtt accgacgtgg
     1441 tactgatcac gcgtgacaac tttaaagaag aactggagaa aaaaggttta ggcggtaagt
     1501 aatttgccgg aaaaattccc ctctgcatga tgcagagggg gtgtgaacga ccagtgattc
     1561 acggagacgt tatgcaacag tctaccccgt atctctcatt tcgcggcatc ggtaaaacgt
     1621 ttcccggcgt taaggcgctg acggatatta gttttgactg ctatgccggt caggttcatg
     1681 cgttgatggg tgaaaatggc gcaggaaaat caactctctt aaaaatcctc agcggcaact
     1741 atgcgccaac cacgggttct gtagtgatta atgggcagga aatgtccttt tccgacacga
     1801 ccgcagcact taacgcgggt gtggcgatta tttaccagga actacatctc gtgccggaaa
     1861 tgaccgtcgc ggaaaacatc tatctcggcc agctgccgca taaaggcggc attgtgaatc
     1921 gctcattgct gaattatgag gcgggtttac aacttaaaca tcttggtatg gatattgacc
     1981 cggacacgcc gctgaaatat ctctccattg gtcagtggca gatggttgaa atcgccaagg
     2041 cgctggcgcg taacgctaaa attatcgcct ttgatgagcc aaccagctcc ctttctgccc
     2101 gcgaaatcga caatcttttc cgcgttattc gtgaactgcg aaaagagggg cgggtgatct
     2161 tatacgtttc tcaccgtatg gaagaaatat ttgccctcag cgatgccatc actgtcttta
     2221 aagatggacg ttatgtcaaa acctttaccg atatgcagca ggttgaccac gacgcgctgg
     2281 tgcaggcgat ggtcgggcgc gacattggcg atatctacgg ctggcaacct cgtagttatg
     2341 gcgaggagcg cctgcgtctt gatgctgtga aagcaccagg cgtgcgtacg ccaataagtc
     2401 tggcggttcg cagtggtgaa attgttgggc tgtttggtct ggtaggggcg gggcgtagcg
     2461 aattaatgaa aggcttgttt ggcgggacgc aaatcaccgc cggtcaggtt tatatcgacc
     2521 aacagccgat cgatattcgt aaaccgagcc acgccattgc cgcaggcatg atgctctgcc
     2581 cggaagatcg caaagcggaa ggcattattc ccgtgcactc cgttcgcgac aatatcaaca
     2641 tcagtgccag acgtaaacat gtgctcggcg gttgtgtaat caacaacggt tgggaagaaa
     2701 acaatgccga tcaccacatt cgttcgctca acatcaaaac gccgggcgct gagcaactga
     2761 tcatgaatct ctcaggcgga aatcagcaaa aagccattct gggccgctgg ttatcggaag
     2821 agatgaaggt cattttgctg gatgaaccta cgcgcggcat tgatgttggt gctaagcatg
     2881 aaatttacaa cgtgatttat gcgctggcgg cgcagggtgt ggcggtgctg tttgcctcca
     2941 gcgacttacc tgaagtcctc ggcgttgccg accggattgt ggtgatgcgg gaaggtgaaa
     3001 tcgccggtga attgttacac gagcaggcag atgagcgtca ggcactgagc cttgcgatgc
     3061 ctaaagtcag ccaggcagtt gcctgagtaa ggagagtatg atgtcttctg tttctacatc
     3121 ggggtctggc gcacctaagt cgtcattcag cttcgggcgt atctgggatc agtacggcat
     3181 gctggtggtg tttgcggtgc tctttatcgc ctgtgccatt tttgtcccaa attttgccac
     3241 cttcattaat atgaaagggt tgggcctggc aatttccatg tcggggatgg tggcgtgtgg
     3301 catgttgttc tgccttgctt ccggtgactt tgacctttct gtcgcctccg taattgcctg
     3361 tgcgggtgtc accacggcgg tggttatcaa cctgactgaa agcctgtgga ttggcgtggc
     3421 agcggggttg ctgcttggca ttctctgtgg cctggtcaat ggctttgtta tcgccaaact
     3481 gaaaataaat gctctgatca caacactggc aacgatgcag attgttcgag gtctggcgta
     3541 catcatttca gacggtaaag cggtcggtat cgaagatgaa agcttctttg cccttggtta
     3601 cgctaactgg ttcggtctgc ctgcgccaat ctggctcacc gtcgcgtgtc tgattatctt
     3661 tggtttgttg ctgaataaaa ccacctttgg tcgtaacacc ctggcgattg gcgggaacga
     3721 agaggctgcg cgtctggcgg gtgtaccggt tgttcgcacc aaaattatta tctttgttct
     3781 ctctggcctg gtatctgcga tagccggaat tattctggct tcacgtatga ctagtgggca
     3841 gccaatgacg tcgattggtt atgagcttat tgttatctcc gcctgcgttt taggtggcgt
     3901 ttctctgaaa ggtggcatcg gaaaaatctc atatgtggtg gcgggtatct taattttagg
     3961 caccgtggaa aacgccatga acctgcttaa tatttctcct ttcgcgcagt acgtggttcg
     4021 cggcttaatc ctgctggcag cggtgatctt cgaccgttac aagcaaaaag cgaaacgcac
     4081 tgtctgatgc ttttttccac aacaatttaa cgttttttcc caccacagcc agccgccaca
     4141 acggttggct gttcttcatt gcaaatggcg acccccgtca cactgtctat acttacatgt
     4201 ctgtaaagcg cgttctgcgc aaaacaataa gaaaagagaa ggaggagagc cgggtgacag
     4261 aaccgttaac cgaaacccct gaactatccg cgaaatatgc ctggtttttt gatcttgatg
     4321 gaacgctggc ggaaatcaaa ccgcatcccg atcaggtcgt cgtgcctgac aatattctgc
     4381 aaggactaca gctactggca accgcaagtg atggtgcatt ggcattgata tcagggcgct
     4441 caatggtgga gcttgacgca ctggcaaaac cttatcgctt cccgttagcg ggcgtgcatg
     4501 gggcggagcg ccgtgacatc aatggtaaaa cacatatcgt tcatctgccg gatgcgattg
     4561 cgcgtgatat tagcgtgcaa ctgcatacag tcatcgctca gtatcccggc gcggagctgg
     4621 aggcgaaagg gatggctttt gcgctgcatt atcgtcaggc tccgcagcat gaagacgcat
     4681 taatgacatt agcgcaacgt attactcaga tctggccaca aatggcgtta cagcagggaa
     4741 agtgtgttgt cgagatcaaa ccgagaggta ccagtaaagg tgaggcaatt gcagctttta
     4801 tgcaggaagc tccctttatc gggcgaacgc ccgtatttct gggcgatgat ttaaccgatg
     4861 aatctggctt cgcagtcgtt aaccgactgg gcggaatgtc agtaaaaatt ggcacaggtg
     4921 caactcaggc atcatggcga ctggcgggtg tgccggatgt ctggagctgg cttgaaatga
     4981 taaccaccgc attacaacaa aaaagagaaa ataacaggag tgatgactat gagtcgttta
     5041 gtcgtagtat ctaaccggat tgcaccacca gacgagcacg ccgccagtgc cggtggcctt
     5101 gccgttggca tactgggggc actgaaagcc gcaggcggac tgtggtttgg ctggagtggt
     5161 gaaacaggga atgaggatca gccgctaaaa aaggtgaaaa aaggtaacat tacgtgggcc
     5221 tcttttaacc tcagcgaaca ggaccttgac gaatactaca accaattctc caatgccgtt
     5281 ctctggcccg cttttcatta tcggctcgat ctggtgcaat ttcagcgtcc tgcctgggac
     5341 ggctatctac gcgtaaatgc gttgctggca gataaattac tgccgctgtt gcaagacgat
     5401 gacattatct ggatccacga ttatcacctg ttgccatttg cgcatgaatt acgcaaacgg
     5461 ggagtgaata atcgcattgg tttctttctg catattcctt tcccgacacc ggaaatcttc
     5521 aacgcgctgc cgacatatga caccttgctt gaacagcttt gtgattatga tttgctgggt
     5581 ttccagacag aaaacgatcg tctggcgttc ctggattgtc tttctaacct gacccgcgtc
     5641 acgacacgta gcgcaaaaag ccatacagcc tggggcaaag catttcgaac agaagtctac
     5701 ccgatcggca ttgaaccgaa agaaatagcc aaacaggctg ccgggccact gccgccaaaa
     5761 ctggcgcaac ttaaagcgga actgaaaaac gtacaaaata tcttttctgt cgaacggctg
     5821 gattattcca aaggtttgcc agagcgtttt ctcgcctatg aagcgttgct ggaaaaatat
     5881 ccgcagcatc atggtaaaat tcgttatacc cagattgcac caacgtcgcg tggtgatgtg
     5941 caagcctatc aggatattcg tcatcagctc gaaaatgaag ctggacgaat taatggtaaa
     6001 tacgggcaat taggctggac gccgctttat tatttgaatc agcattttga ccgtaaatta
     6061 ctgatgaaaa tattccgcta ctctgacgtg ggcttagtga cgccactgcg tgacgggatg
     6121 aacctggtag caaaagagta tgttgctgct caggacccag ccaacccggg cgttcttgtt
     6181 ctttcgcaat ttgcgggagc ggcaaacgag ttaacgtcgg cgttaattgt taacccctac
     6241 gatcgtgacg aagttgcagc tgcgctggat cgtgcattga ctatgtcgct ggcggaacgt
     6301 atttcccgtc atgcagaaat gctggacgtt atcgtgaaaa acgatattaa ccactggcag
     6361 gagtgcttca ttagcgacct aaagcagata gttccgcgaa gcgcggaaag ccagcagcgc
     6421 gataaagttg ctacctttcc aaagcttgcg taggagcggt taatctcccg taagtggaac
     6481 taacagcaca tcgacctcac ttgaggcaat aactcttttc gccgagcagg atgctcgcga
     6541 aaagaaactg tgattgtgat taccgcaaat caccaaatcg aaatgatgtt tgtgacatac
     6601 ttccagaata tgttcgctta actcaccgta ggcaataaac gtcttgtcaa cgggataccc
     6661 cgcatcctga attaacttat caagaaagct ttgcgtttct tcttgcatca cgctgcgtaa
     6721 atcttcaagc atcggcgcgg ctaattgatt gtacatttcc gggtcggaag cgagagtaat
     6781 taaactgatg tgtcccttta ctggcctggc gatagatacc gcttttgcca gcagttgctg
     6841 actttccggt gtaaccgcaa cagcgacaag aatattgcta tagctcataa cctgttcctt
     6901 attctgtgaa cttcaggtga cattaaagct ttactcctaa tggagaaacg acgcaatccc
     6961 aactcgg
//
LOCUS       DAZKHF020000228         6892 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2201S31241
            SAMN43267800-rid25106413.denovo.230, whole genome shotgun sequence.
ACCESSION   DAZKHF020000228 DAZKHF020000000
VERSION     DAZKHF020000228.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267800
            Sequence Read Archive: SRR30307263
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6892)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6892)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 6892)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350581.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350581.2
            Genome Coverage       :: 151x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:21:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,652
            CDSs (total)                      :: 5,557
            Genes (coding)                    :: 5,320
            CDSs (with protein)               :: 5,320
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 237
            CDSs (without protein)            :: 237
            Pseudo Genes (ambiguous residues) :: 0 of 237
            Pseudo Genes (frameshifted)       :: 102 of 237
            Pseudo Genes (incomplete)         :: 130 of 237
            Pseudo Genes (internal stop)      :: 52 of 237
            Pseudo Genes (multiple problems)  :: 41 of 237
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6892
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267800-rid25106413.denovo.230"
                     /serotype="O 26:H11"
                     /isolate="2201S31241"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-01"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            complement(199..684)
                     /gene="sixA"
                     /locus_tag="ACHOSC_RS19170"
                     /old_locus_tag="pgaptmp_003834"
     CDS             complement(199..684)
                     /gene="sixA"
                     /locus_tag="ACHOSC_RS19170"
                     /old_locus_tag="pgaptmp_003834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0101006 - protein histidine phosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphohistidine phosphatase SixA"
                     /protein_id="HGU4549683.1"
                     /translation="MQVFIMRHGDAALDAASDSVRPLTTNGCDESRLMANWLKGQKVE
                     IERVLVSPFLRAEQTLEEVGDCLNLPSSAEVLPELTPCGDVGLVSAYLQALTNEGVAS
                     VLVISHLPLVGYLVAELCPGETPPMFTTSAIASVTLDESGNGTFNWQMSPCNLKMAKA
                     I"
     gene            complement(887..3031)
                     /gene="fadJ"
                     /locus_tag="ACHOSC_RS19175"
                     /old_locus_tag="pgaptmp_003835"
     CDS             complement(887..3031)
                     /gene="fadJ"
                     /locus_tag="ACHOSC_RS19175"
                     /old_locus_tag="pgaptmp_003835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003857 - 3-hydroxyacyl-CoA dehydrogenase
                     activity [Evidence IEA];
                     GO_function: GO:0004300 - enoyl-CoA hydratase activity
                     [Evidence IEA];
                     GO_function: GO:0008692 - 3-hydroxybutyryl-CoA epimerase
                     activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0009062 - fatty acid catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acid oxidation complex subunit alpha FadJ"
                     /protein_id="HGU4549684.1"
                     /translation="MEMTSAFTLNVRLDNIAVITIDVPGEKMNTLKAEFASQVRAIIK
                     QLRENKELRGVVFVSAKPDNFIAGADINMIGNCKTAQEAEALARQGQQLMAEIHALPI
                     PVIAAIHGACLGGGLELALACHGRVCTDDPKTVLGLPEVQLGLLPGSGGTQRLPRLIG
                     VSTALEMILTGKQLRAKQALKLGLVDDVVPHSILLEVAVELAKKDRPSSRPLPVRERI
                     LAGPLGRALLFKMVGKKTEHKTQGNYPATERILEVVETGLAQGTSSGYDAEARAFGEL
                     AMTPQSQALRSIFFASTEVKKDPGSDAPPAPLNSVGILGGGLMGGGIAYVTACKAGLP
                     VRIKDINPQGINHALKYSWDQLEGKVRRRHLKASERDKQLALISGTTDYRGFAHRDLI
                     IEAVFENLELKQQMVAEVEQNCAAHTIFASNTSSLPIGDIAAHATRPEQVIGLHFFSP
                     VEKMPLVEIIPHAGTSAQTIATTVKLAKKQGKTPIVVRDKAGFYVNRILAPYINEAIR
                     MLTEGERVEHIDAALVKFGFPVGPIQLLDEVGIDTGTKIIPVLEAAYGERFSAPANVV
                     SSILNDDRKGRKNGRGFYLYGQKGRKSKKQVDPAIYPLIGAQGQGRLSAPQVAERCVM
                     LMLNEAVRCVDEQVIRSVRDGDIGAVFGIGFPPFLGGPFRYIDSLGAGEVVAIMQRLA
                     TQYGSRFTPCERLVEMGARGESFWKTTATDLQ"
     gene            complement(3031..4341)
                     /gene="fadI"
                     /locus_tag="ACHOSC_RS19180"
                     /old_locus_tag="pgaptmp_003836"
     CDS             complement(3031..4341)
                     /gene="fadI"
                     /locus_tag="ACHOSC_RS19180"
                     /old_locus_tag="pgaptmp_003836"
                     /EC_number="2.3.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003988 - acetyl-CoA C-acyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006631 - fatty acid metabolic process
                     [Evidence IEA];
                     GO_process: GO:0016042 - lipid catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acyltransferase FadI"
                     /protein_id="HGU4549685.1"
                     /translation="MGQVLPLVTRQGDRIAIVSGLRTPFARQATAFHGIPAVDLGKMV
                     VGELLARSEIPAEVIEQLVFGQVVQMPEAPNIAREIVLGTGMNVHTDAYSVSRACATS
                     FQAVANVAESLMAGTIRAGIAGGADSSSVLPIGVSKKLARVLVDVNKARTMSQRLKLF
                     SRLRLRDLMPVPPAVAEYSTGLRMGDTAEQMAKTYGITREQQDALAHRSHQRAAQAWS
                     DGKLKEEVMTAFIPPYKQPLVEDNNIRGNSSLADYAKLRPAFDRKHGTVTAANSTPLT
                     DGAAAVILMTESRAKELGLVPLGYLRSYAFTAIDVWQDMLLGPAWSTPLALERAGLTM
                     SDLTLIDMHEAFAAQTLANIQLLGSERFAREVLGRAHATGEVDDSKFNVLGGSIAYGH
                     PFAATGARMITQTLHELRRRGGGFGLVTACAAGGLGAAMVLEAE"
     gene            complement(4521..4805)
                     /locus_tag="ACHOSC_RS19185"
                     /old_locus_tag="pgaptmp_003837"
     CDS             complement(4521..4805)
                     /locus_tag="ACHOSC_RS19185"
                     /old_locus_tag="pgaptmp_003837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfcZ/YiiS family protein"
                     /protein_id="HGU4549686.1"
                     /translation="MSKCSADETPVCCCMDVGTIMDNSDCTASYSRVFASRAEAEQTL
                     AALTEKARSVESEPCKITPTFTEESDGVRLDIDFTFACEAEMLIFQLGLR"
     gene            5177..6517
                     /gene="fadL"
                     /locus_tag="ACHOSC_RS19190"
                     /old_locus_tag="pgaptmp_003838"
     CDS             5177..6517
                     /gene="fadL"
                     /locus_tag="ACHOSC_RS19190"
                     /old_locus_tag="pgaptmp_003838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708225.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long-chain fatty acid transporter FadL"
                     /protein_id="HGU4549687.1"
                     /translation="MSQKTLFTKSALAVAVALISTQAWSAGFQLNEFSSSGLGRAYSG
                     EGAIADDAGNVSRNPALITMFDRPTFSAGAVYIDPDVNISGTSPSGRSLKADNIAPTA
                     WVPNMHFVAPINDQFGWGASITSNYGLATEFNDTYAGGSVGGTTDLETMNLNLSGAYR
                     LNNAWSFGLGFNAVYARAKIERFAGDLGQLVAGQIMQSPAGQTPQGQALAATANGIDS
                     NTKIAHLNGNQWGFGWNAGILYELDKNNRYALTYRSEVKIDFKGNYSSDLNRAFNNYG
                     LPIPTATGGATQSGYLTLNLPEMWEVSGYNRVDPQWAIHYSLAYTSWSQFQQLKATST
                     SGDTLFQKHEGFKDAYRIALGTTYYYDDNWTFRTGIAFDDSPVPAQNRSISIPDQDRF
                     WLSAGTTYAFNKDASVDVGVSYMHGQSVKINEGPYQFESEGKAWLFGTNFNYAF"
BASE COUNT         1727 a         1839 c         1630 g         1696 t
ORIGIN      
        1 acagacatta ttcatttcaa catgtaactt tgggcaaaat caagatacgc gcagaagaat
       61 aatttccgaa tgaaagtaga aagtgtttta tttatagatg gggagagtct tcgatagtag
      121 tgaggttgac agatatccgc tgcctgatga gcaattatgc ccaatgaatc tacctcattg
      181 ggcataattt ggaactcatc agatagcttt tgccatcttc agattacacg ggctcatctg
      241 ccagttgaat gtgccgtttc cgctttcatc aagcgttacg ctggcaatgg ccgaggtggt
      301 aaacataggc ggcgtttcgc ccggacataa ctcggcaacc agatacccga ctaacggtaa
      361 gtgagagatc accagtaccg aagcaacacc ttcattggtc aacgcctgca aataggcact
      421 gaccagacca acatcgccgc agggcgttaa ctccggcaga acttctgcac tggaaggcag
      481 gttcagacaa tcccctactt cttccagtgt ttgctcggct cgcaggaacg ggctcaccag
      541 aacgcgttcg atttccactt tttgaccttt cagccagttc gccatcaggc gagattcgtc
      601 acaaccatta gtggtcagag gacgaacgga atcactggcg gcatcgaggg ctgcgtcgcc
      661 gtgacgcatg ataaaaactt gcatattgca ccgcttttgt taaccagatt cgcccgtttt
      721 ctttcatctg aaaccagaga aagaaaacgg tggccggcat tgtgccttat ccattcaccg
      781 aatgaaacgc tgttttttac ctcaatggcg taagtatagt caatatgcgt ttatattttg
      841 accaacactc cgccattcag cgcggattca tatagctttg accttcttat tgcaggtcag
      901 ttgcagttgt tttccaaaaa ctttccccac gcgcgcccat ctcgaccaaa cgctcgcaag
      961 gggtaaaacg ggaaccatac tgcgtggcaa gtcgttgcat tattgcaacc acttcgcccg
     1021 cgccgagaga atcgatatag cggaacggtc caccgagaaa tggcggaaaa ccaatgccaa
     1081 ataccgcacc aatatccccg tcacgcacgc tacggataac ctgctcatca acacaacgta
     1141 ctgcttcatt cagcatcaac atcacacacc gttcagcaac ctgcggtgcg gagagtcgcc
     1201 cctgcccttg tgcgccaatg agcgggtaaa tggcgggatc gacctgtttt ttgcttttac
     1261 gccctttctg accataaaga tagaaacccc gaccattttt tctgcctttg cgatcgtcgt
     1321 tcaaaattga agaaacaaca tttgcaggcg cgctaaaacg ttctccataa gcggcttcca
     1381 gtacaggaat aattttagtc ccggtgtcga ttcctacctc atccaaaagt tggattggac
     1441 ctaccggaaa accaaatttc actagcgcgg catcaatgtg ctctacccgt tcaccttcgg
     1501 tcagcatgcg gatagcttca ttaatgtaag gcgctaagat gcgattgacg taaaacccgg
     1561 ctttgtcacg cacgacaatg ggcgttttgc cctgtttttt cgccagtttt accgtagtgg
     1621 cgatggtttg cgccgatgta cccgcatgag gaataatctc taccagcggc attttttcca
     1681 ccggactgaa gaaatgcagg ccaataactt gctcaggtcg cgtggcgtga gctgcgatat
     1741 caccaatcgg taaagatgac gtattcgaag caaaaatggt atgagcggcg caattttgct
     1801 ccacttccgc caccatctgt tgttttaatt cgagattttc aaacaccgct tcaataatca
     1861 gatcgcgatg ggcaaagccg cgatagtccg tcgttccgga gattaatgcc agctgtttgt
     1921 cacgttcgct ggctttgaga tgacgacggc gaactttgcc ctccagctga tcccaactgt
     1981 acttcagcgc atgatttatg ccctgcgggt tgatatcttt aattctgacc ggaagccccg
     2041 ctttacaagc agtgacataa gcaataccgc cgcccatcaa gccaccacct aaaattccca
     2101 cgctgtttaa tggcgcaggc ggtgcatcac taccgggatc tttcttcact tccgtactgg
     2161 caaaaaagat actacgcaaa gcttgcgatt gtggcgtcat cgccagttcg ccaaacgccc
     2221 gagcttcggc gtcataaccg ctgctggtgc cctgcgctaa tcccgtttca acaacctcca
     2281 ggatgcgttc tgtcgccgga taattgcctt gagttttgtg ttctgttttc ttgccgacca
     2341 ttttgaacag cagcgcacga cctaacggcc ccgccagaat acgctcgcgt acaggtagag
     2401 ggcgggaaga tgggcgatcc ttcttcgcca gctcaacagc gacttccagc aaaatggagt
     2461 gcggaacaac gtcatccacc agccccagct ttaatgcctg tttcgcccga agttgttttc
     2521 cggtgaggat catctctaat gctgtgctga cgcctatcag acgcggtaaa cgctgggtgc
     2581 cgcctgaacc gggtaacaat ccaagttgta cttcaggcaa accgagcacc gttttaggat
     2641 cgtcagtaca aacgcgaccg tggcacgcca gcgccaactc cagcccacca cccaggcaag
     2701 cgccatgaat agccgcgata accggaatgg gcaaagcatg aatctccgcc atcaactgtt
     2761 ggccctgccg cgccagtgct tccgcttctt gcgccgtttt gcagttgccg atcatgttga
     2821 tgtctgcgcc agcaatgaag ttgtccggtt tagcggagac aaacaccacg cctcgcaact
     2881 ctttgttttc acggagttgc ttaataatgg cgcgcacctg cgaggcaaac tccgccttca
     2941 gggtattcat tttctcaccc ggtacgtcga tggtgataac ggcaatgttg tccagacgaa
     3001 cattaagggt aaacgctgat gtcatttcca ttattccgcc tccagaacca ttgccgcgcc
     3061 aagcccaccg gcagcacagg cggtaactaa accaaatcca ccgccgcgac ggcgaagttc
     3121 atgcaatgtc tgggtaatca tccgtgcgcc ggtcgccgcg aagggatgtc cgtaagcaat
     3181 cgaaccgcca agcacgttaa atttgctatc gtccacttcg ccagtggcat gtgcacgccc
     3241 cagtacttca cgagcaaaac gttcactacc cagcaactga atattcgcca gtgtctgagc
     3301 tgcaaaggct tcgtgcatat cgatcaatgt cagatcgctc attgtcaaac cggcacgttc
     3361 cagcgccagc ggtgttgacc aggccggacc gagcaacatg tcctgccaga catcaatcgc
     3421 agtaaatgcg tagctgcgca gataccccag cggcaccagc cctaattctt tcgcccggga
     3481 ttcagtcatc aggatcaccg ctgccgcgcc atcggtcagc ggcgtactgt ttgccgccgt
     3541 taccgttccg tgtttgcgat caaacgccgg gcgcagcttt gcgtaatcgg caagcgagga
     3601 attaccgcga atattgttgt cttcgacaag cggttgttta taaggaggga taaaggcagt
     3661 catcacctct tctttgagtt ttccgtctga ccatgcctga gcggcacgct gatgcgaacg
     3721 gtgcgctaat gcatcttgct gttctcgggt gatgccgtag gttttcgcca tttgttcggc
     3781 ggtgtcgccc atccgcaagc cagtagaata ttctgctacc gcaggcggta cgggcattaa
     3841 gtcgcgcaaa cgcaggcgag agaagagttt cagtcgctgg ctcatggttc gagctttgtt
     3901 gacatcaacc agcacgcgcg ccagtttttt actgacgcca attggcaata cggaagagga
     3961 atctgcccca ccggcaatcc ccgctcggat agttcccgcc atcaggcttt ctgcgacgtt
     4021 tgcaaccgcc tggaaactgg tagcgcaagc gcggctgacg ctgtaagcat cggtatgcac
     4081 attcattccg gtaccgagaa caatttcacg cgcaatgttg ggggcttcag gcatttgtac
     4141 gacctgacca aagaccagct gttcaatcac ttcagcgggg atctcgctgc gtgccagcag
     4201 ttcgcctacc accatcttcc ctaaatcaac cgcgggaatg ccatgaaaag ccgtcgcctg
     4261 gcgggcaaaa ggcgtacgta aaccgctaac aatggcgata cgatcgccct ggcgggtaac
     4321 cagcggtaaa acctgaccca taacactccc ctgtaaaaaa ataaataaag tggtctgacc
     4381 tgatcatagt cttaaccatt tttttacatt tagccaagcg gagaaaaagg aaagtgggag
     4441 ctatgacaca gtgaagacag gagaaagaaa agaaaacgcc cctgccagca actgacaggg
     4501 gcgtacgtac ccgagaggaa ttaacgcaga cccagctgga agatcagcat ttcagcttcg
     4561 caggcaaaag tgaaatcgat atccaggcgc acaccgtcag actcttcagt gaaagtcggg
     4621 gtgattttac acggttcgga ttccacgcta cgggcttttt cagtcagcgc cgccagcgtt
     4681 tgttctgctt ctgcgcggct tgcaaacacg cggctgtaag acgcggtgca gtcggagttg
     4741 tccataatgg tgccaacatc catacagcag caaaccgggg tttcatcagc actacattta
     4801 ctcatcgttg atttcctctg tatgtgcacc caaggtgcca gataaacgtt gtggatattt
     4861 tacgcttccg gaaagtgctg ctccagttgt taattctgca aaatcggata agtgaccgaa
     4921 atcacactta aaaatgatct aaaacaaaat tcacccgaat ccgcgagtgc gcaacctcca
     4981 aattttgcca gctggatcgc gtttcttaga tcatatttga aaaaagatgg aaacatactt
     5041 gcaacattcc agctggtccg acctatactc tcgccactgg tctgatttct aagatgtacc
     5101 tcagacccta cacttcgcgc tcctgttaca gcacgtaaca tagtttgtat aaaaataaat
     5161 cattgaggtt atggtcatga gccagaaaac cctgtttaca aagtctgctc tcgcagtcgc
     5221 agtggcactt atctccaccc aggcctggtc ggcaggcttt cagttaaacg aattttcttc
     5281 ctctggcctg ggccgggctt attcagggga aggcgcaatt gccgatgatg caggtaacgt
     5341 tagccgtaac cccgcattga ttaccatgtt tgaccgcccg acattttctg cgggtgcggt
     5401 ttatattgac ccggatgtga atatcagcgg aacgtctccg tctggtcgta gcctgaaagc
     5461 cgataacatc gcgcctacgg catgggttcc gaacatgcac tttgttgcac cgattaacga
     5521 ccaatttggt tggggcgctt ctattacctc taactatggt ctggcaacag agtttaatga
     5581 tacttatgca ggcggctctg tcgggggtac aactgacctt gaaaccatga acctgaactt
     5641 aagcggtgcg tatcgcttaa ataatgcatg gagctttggt cttggtttca acgccgtcta
     5701 cgctcgcgcg aaaattgaac gtttcgcagg cgatctgggg cagttggttg ctggtcagat
     5761 tatgcaatct cctgccgggc aaactcctca agggcaagca ttggcagcta ccgccaacgg
     5821 tatcgacagt aataccaaaa tcgctcatct gaacggtaac cagtggggct ttggctggaa
     5881 cgccggaatc ctgtatgaac tggataaaaa taaccgctat gcactgacct accgttctga
     5941 agtgaaaatt gacttcaaag gtaactacag cagcgatctt aatcgtgcgt ttaataacta
     6001 cggtttgcca attcctaccg cgacaggtgg cgcaacgcaa tcgggttatc tgacgctgaa
     6061 cctgcctgaa atgtgggaag tgtcaggtta taaccgtgtt gatccacagt gggcgattca
     6121 ctatagcttg gcttacacca gctggagtca gttccagcag ctgaaagcga cctcaaccag
     6181 tggcgacacg ctgttccaga aacatgaagg ctttaaagat gcttaccgca tcgcgttggg
     6241 taccacttat tactacgatg ataactggac cttccgtacc ggtatcgcct ttgatgacag
     6301 cccagttccg gctcagaatc gttctatctc cattccggac caggaccgtt tctggctgag
     6361 tgcaggtacg acttacgcat ttaataaaga tgcttcagtc gacgttggtg tttcttatat
     6421 gcacggtcag agcgtgaaaa ttaacgaagg cccataccag ttcgagtctg aaggtaaagc
     6481 ctggctgttc ggtactaact ttaactacgc gttctgataa cgcgttcgcc tggataaagt
     6541 cacctgcata gcaggtgact ttaactcccc cacttcaccg aaagtagtgt cctcattgct
     6601 tacctcctga gttttgcaaa cagcctgatg gcagcttgcc ctttcaaatc aataagcggt
     6661 atccacagac aaatctgaag ggaaaggcat attttcaggc gttctgctcg tccttctcaa
     6721 agagtttact tttctgaatt tccaggatac tcacccccct ggctattgtg cgctcataca
     6781 ctcaaattaa agataggttc taaataaatg agcgtttttt gatagtctat ttcattaggt
     6841 aatatatatt tgtaacaaat caatcaaaat ggaataaaat catgctacca tc
//
LOCUS       DAZKHF020000229         6877 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2201S31241
            SAMN43267800-rid25106413.denovo.231, whole genome shotgun sequence.
ACCESSION   DAZKHF020000229 DAZKHF020000000
VERSION     DAZKHF020000229.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267800
            Sequence Read Archive: SRR30307263
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6877)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6877)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 6877)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350581.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350581.2
            Genome Coverage       :: 151x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:21:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,652
            CDSs (total)                      :: 5,557
            Genes (coding)                    :: 5,320
            CDSs (with protein)               :: 5,320
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 237
            CDSs (without protein)            :: 237
            Pseudo Genes (ambiguous residues) :: 0 of 237
            Pseudo Genes (frameshifted)       :: 102 of 237
            Pseudo Genes (incomplete)         :: 130 of 237
            Pseudo Genes (internal stop)      :: 52 of 237
            Pseudo Genes (multiple problems)  :: 41 of 237
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6877
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267800-rid25106413.denovo.231"
                     /serotype="O 26:H11"
                     /isolate="2201S31241"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-01"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            complement(<1..621)
                     /locus_tag="ACHOSC_RS19195"
                     /old_locus_tag="pgaptmp_003839"
     CDS             complement(<1..621)
                     /locus_tag="ACHOSC_RS19195"
                     /old_locus_tag="pgaptmp_003839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1076 domain-containing protein"
                     /protein_id="HGU4549688.1"
                     /translation="MPVILNFSNGSVLPENELEALRHIARSNQNDTITIGGRNMRLHY
                     IQFMDGFSVEPIPGGLWDHLGARESHHLADSLARQLNGGNTFLQAYSLYLEQRQAAPL
                     VQESVIKTLLDRINSNAFPVSLQDFSCTEEHLNCPITLHIPETGVFVRNARNSEICAL
                     YDQEALTELILRNALHPLSRDPFAPEMIISKDKCHFNITKQCFYALP"
     gene            805..1395
                     /gene="espM2"
                     /locus_tag="ACHOSC_RS19200"
                     /old_locus_tag="pgaptmp_003840"
     CDS             805..1395
                     /gene="espM2"
                     /locus_tag="ACHOSC_RS19200"
                     /old_locus_tag="pgaptmp_003840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T3SS effector guanine nucleotide exchange factor
                     EspM2"
                     /protein_id="HGU4549689.1"
                     /translation="MPMNTSGTSFSSFGISCHREGSFRNSFRGKNDEVVKCSIGERTI
                     SFSVRKFSRNILDTVNRQSAKDIDGWIKDERIVYPSRVINQEIDNYCFQKNAKISTEE
                     RQRVFSLVSQENQLTLDVKAAQSSINHVIMGSASFGKKMDALCDGMSRDVKNRTSDTI
                     ANLLADKFYQKHIDSDIDIVKLRNDIPAYLTRAIQG"
     gene            complement(1418..1503)
                     /locus_tag="ACHOSC_RS19205"
                     /old_locus_tag="pgaptmp_003841"
                     /pseudo
     CDS             complement(1418..1503)
                     /locus_tag="ACHOSC_RS19205"
                     /old_locus_tag="pgaptmp_003841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414822.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="YkgJ family cysteine cluster protein"
     gene            1627..1776
                     /locus_tag="ACHOSC_RS19210"
                     /old_locus_tag="pgaptmp_003842"
                     /pseudo
     CDS             1627..1776
                     /locus_tag="ACHOSC_RS19210"
                     /old_locus_tag="pgaptmp_003842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000885598.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tail fiber assembly protein"
     gene            complement(2009..2872)
                     /gene="potB"
                     /locus_tag="ACHOSC_RS19215"
                     /old_locus_tag="pgaptmp_003843"
     CDS             complement(2009..2872)
                     /gene="potB"
                     /locus_tag="ACHOSC_RS19215"
                     /old_locus_tag="pgaptmp_003843"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309597.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="spermidine/putrescine ABC transporter permease
                     PotB"
                     /protein_id="HGU4549690.1"
                     /translation="MKNTSKFQNVVIVTIVGWLVLFVFLPNLMIIGTSFLTRDDASFV
                     KMVFTLDNYTRLLDPLYFEVLLHSLNMALIATLACLVLGYPFAWFLAKLPHKVRPLLL
                     FLLIVPFWTNSLIRIYGLKIFLSTKGYLNEFLLWLGVIDTPIRIMFTPSAVIIGLVYI
                     LLPFMVMPLYSSIEKLDKPLLEAARDLGASKLQTFIRIIIPLTMPGIIAGCLLVMLPA
                     MGLFYVSDLMGGAKNLLIGNVIKVQFLNIRDWPFGAATSITLTIVMGLMLLVYWRASR
                     LLNKKVNELDD"
     gene            complement(2856..3992)
                     /gene="potA"
                     /locus_tag="ACHOSC_RS19220"
                     /old_locus_tag="pgaptmp_003844"
     CDS             complement(2856..3992)
                     /gene="potA"
                     /locus_tag="ACHOSC_RS19220"
                     /old_locus_tag="pgaptmp_003844"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015846 - polyamine transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="spermidine/putrescine ABC transporter
                     ATP-binding protein PotA"
                     /protein_id="HGU4549691.1"
                     /translation="MGQSKKLNKQPSSLSPLVQLAGIRKCFDGKEVIPQLDLTINNGE
                     FLTLLGPSGCGKTTVLRLIAGLETVDSGRIMLDNEDITHVPAENRYVNTVFQSYALFP
                     HMTVFENVAFGLRMQKTPAAEITPRVMEALRMVQLETFAQRKPHQLSGGQQQRVAIAR
                     AVVNKPRLLLLDESLSALDYKLRKQMQNELKALQRKLGITFVFVTHDQEEALTMSDRI
                     VVMREGRIEQDGTPREIYEEPKNLFVAGFIGEINMFNATVIERLDEQRVRANVEGREC
                     NIYVNFAVEPGQKLHVLLRPEDLRVEEINDDNHAEGLIGYVRERNYKGMTLESVVELE
                     NGKMVMVSEFFNEDDPDFDHSLDQKMAINWVESWEVVLADEEHK"
     gene            4242..5471
                     /gene="pepT"
                     /locus_tag="ACHOSC_RS19225"
                     /old_locus_tag="pgaptmp_003845"
     CDS             4242..5471
                     /gene="pepT"
                     /locus_tag="ACHOSC_RS19225"
                     /old_locus_tag="pgaptmp_003845"
                     /EC_number="3.4.11.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000359435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0045148 - tripeptide aminopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase T"
                     /protein_id="HGU4549692.1"
                     /translation="MDKLLERFLNYVSLDTQSKAGVRQVPSTEGQWKLLHLLKEQLEE
                     MGLINVTLSEKGTLMATLPANVPGDIPAIGFISHVDTSPDCSGKNVNPQIVENYRGGD
                     IALGIGDEVLSPVMFPVLHQLLGQTLITTDGKTLLGADDKAGIAEIMTALAVLQQKNI
                     PHGDIRVAFTPDEEVGKGAKHFDVDAFDARWAYTVDGGGVGELEFENFNAASVNIKIV
                     GNNVHPGTAKGVMVNALSLAARIHAEVPADESPEMTEGYEGFYHLASMKGTVDRADMH
                     YIIRDFDRKQFEARKRKMMEIAKKVGKGLHPDCYIELVIEDSYYNMREKVVEHPHIID
                     IAQQAMRDCDIEPELKPIRGGTDGAQLSFMGLPCPNLFTGGYNYHGKHEFVTLEGMEK
                     AVQVIVRIAELTAQRKS"
     gene            complement(5617..6738)
                     /gene="roxA"
                     /locus_tag="ACHOSC_RS19230"
                     /old_locus_tag="pgaptmp_003846"
                     /gene_synonym="ycfD"
     CDS             complement(5617..6738)
                     /gene="roxA"
                     /locus_tag="ACHOSC_RS19230"
                     /old_locus_tag="pgaptmp_003846"
                     /gene_synonym="ycfD"
                     /EC_number="1.14.11.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415646.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="[50S ribosomal protein L16]-arginine
                     3-hydroxylase"
                     /protein_id="HGU4549693.1"
                     /translation="MEYQLTLNWPDFLERHWQKRPVVLKRGFNNFIDPISPDELAGLA
                     MESEVDSRLVSHQDGKWQVSHGPFESYDHLGETNWSLLVQAVNHWHEPTAALMRPFRE
                     LPDWRIDDLMISFSVPGGGVGPHLDQYDVFIIQGTGRRRWRVGEKLQMKQHCPHPDLL
                     QVDPFEAIIDEELEPGDILYIPPGFPHEGYALENAMNYSVGFRAPNTRELISGFADYV
                     LQRELGGNYYSDPDVPPRAHPADVLPQEMDKLREMMLELINQPEHFKQWFGEFISQSR
                     HELDIAPPEPPYQPDEIYDALKQGEVLVRLGGLRVLRIGDDVYANGEKIDSPHRPALD
                     ALASNIALTAENFGDALEDPSFLAMLAALVNSGYWFFEG"
BASE COUNT         1874 a         1581 c         1678 g         1744 t
ORIGIN      
        1 gggtaatgcg taaaaacatt gttttgttat attaaaatga cacttgtctt tgcttataat
       61 catttctgga gcaaaggggt cacggctgag ggggtggaga gcgttacgca ggataagttc
      121 agtcaatgct tcctgatcat ataatgcaca tatttctgaa tttcgagcat ttctgacaaa
      181 aacacctgtc tcaggaatat gtagcgttat cggacaatta agatgctcct cagtacagga
      241 aaagtcttgt aaactaacgg gaaatgcatt cgaatttatt cgatctagta gtgtttttat
      301 gacgctttcc tgcacaagcg gggcagcttg cctctgctcc agatataagc tgtatgcctg
      361 cagaaaagtg ttacctccgt taagttgtct tgccaggctg tctgcgagat gatgagactc
      421 tcttgcccca agatggtccc aaagtccacc aggaataggc tctacactaa aaccatccat
      481 aaactggata taatgaagcc tcatattacg gcctcctata gtaatagtgt cattctgatt
      541 gcttcttgca atatgtcgta aagcctccag ctcattttcc ggcaatacac ttccattaga
      601 aaaatttaat atgactggca tattattatc cttttcaacc cattttgtga actatattgg
      661 taacaagagt caacataccg atcatgagat gggactcaaa caatacaact tttaatagaa
      721 tattatttgc atgcatgttt tttctggcta caataaacac aagcatgcaa tcttgtaaac
      781 gcttttttat aaaaaggaat tataatgccg atgaatactt caggtacgtc tttcagctct
      841 tttggtataa gttgccatag agaaggctcc tttaggaact ctttccgagg gaagaatgat
      901 gaggtcgtaa aatgttcaat tggagaacgg actattagct tttctgttcg caaatttagc
      961 agaaatatac tggatacagt gaacaggcag agtgctaaag atattgatgg gtggataaaa
     1021 gatgaacgca tagtatatcc ctcaagggtg atcaaccagg agattgataa ttactgtttt
     1081 caaaaaaatg caaaaatttc cactgaagag aggcaaagag tcttttctct tgtgagccag
     1141 gagaatcaac taacccttga tgttaaggca gcacaaagtt ctattaatca tgtaataatg
     1201 gggagtgctt cttttggcaa gaaaatggat gctctttgtg atggtatgag ccgggatgta
     1261 aaaaatcgta catcagatac catagcaaac ttgcttgcgg acaagtttta ccagaaacat
     1321 atcgattcgg atattgatat tgtaaaacta cgaaatgata tcccagctta cttgacgcgt
     1381 gctatacagg gatgaggcta gaggagagga ctgcttcagc atcatcggct tcggaccagt
     1441 aaaagagacg cagaaaaatg cacaacaggc accacacgcc atgcatggat tcagattact
     1501 catggctcac cagtacagct ataaatcgta aagaaaacta cagtacgttg tacacagaaa
     1561 cgtagcgtga ttcactattg cacaggtcca tcacaacgac aatattttgt gatgtttgcg
     1621 tggtgcgccc cacttcagga tgctgcagat ctggagatcg taattgagga agagacttca
     1681 ttgcgggaag cctggaaaaa gtatcgggtg ttgttgaatc gtgttgatac atcagcagca
     1741 cctgatattg agtggccgac ttcacctgta gagtaatcaa cggagcaggg atgatatttt
     1801 gtgcttgaag tgcctccccg accattcaga tagtcgggga gggggtattg cgattatacg
     1861 caatcagttt tagttaaaga ccgaagctct cgaaaaatct ttctccaaaa ccagaatgca
     1921 attgattgat tttgatagga tctataacgc cgttacctta aacgacaaaa tcactcaaaa
     1981 acaaacctaa taaattgaat ataaaggatt aatcatctaa ttcgttcacc ttcttattca
     2041 gcagacgaga agcgcgccag taaaccagca acatcaggcc cattacgata gtcagcgtaa
     2101 tgctggtggc tgcaccaaat ggccagtcac gaatattgag gaactgtacc ttgatgacgt
     2161 taccgatcag caggtttttc gcaccgccca tcaggtcgga tacatagaac aggcccatcg
     2221 ctggcagcat caccagcaga catccggcaa taattcccgg catcgtcagt ggaataatga
     2281 tacggataaa agtctgtaac ttgctggcac cgagatcgcg cgccgcctca agcaacggct
     2341 tatccagttt ttcgatactg gagtacagcg gcatcaccat aaacggcagc agaatgtaaa
     2401 ccagaccgat aatcaccgca ctgggcgtga acatgatacg gattggtgtg tcgataacgc
     2461 ccagccagag caaaaactcg ttgagatagc ctttggtgct gaggaaaatt ttcagcccgt
     2521 agatacgaat caatgagttg gtccagaagg gaacaatcag cagaaacagc agcagcggac
     2581 gcaccttgtg tggcagcttc gccagaaacc aggcaaacgg gtagcccagc accaggcagg
     2641 cgagggtggc gatcagcgcc atattcagcg agtgcaatag cacttcaaaa tagagcggat
     2701 cgagcagacg cgtgtagtta tccagcgtaa agaccatttt tacgaaactg gcgtcgtcgc
     2761 gggtcaaaaa gctggtgcca atgatcatca ggttgggcag aaagacaaac aacacaagcc
     2821 aaccgacaat agtgacaatc actacattct ggaacttact tgtgttcttc atcagccagt
     2881 acgacctccc agctttctac ccaattaatg gccatttttt ggtcgagaga gtggtcaaag
     2941 tcaggatcgt cttcattgaa gaattcgctg accatcacca tcttgccatt ttccagttca
     3001 acaaccgact ccagcgtcat gcctttgtag ttacgctcac gaacgtaacc aatcagccct
     3061 tcagcgtggt tgtcgtcgtt aatctcttca acacgtaagt cttccgggcg cagcagaaca
     3121 tgcagttttt gccccggttc aacggcgaag ttaacgtaga tattacattc gcggccttca
     3181 acgttggcgc gtacgcgctg ctcgtctaga cgttcgatga cagtggcgtt aaacatattg
     3241 atttcgccaa tgaagccggc aacaaacagg tttttcggct cttcgtaaat ttcgcgcggt
     3301 gtaccatctt gctcaatacg accctcgcgc atcaccacaa tcctgtctga catggtgagc
     3361 gcttcttcct gatcgtgagt gacaaagacg aaggtaatac caagcttacg ctgtaacgct
     3421 ttcagctcgt tctgcatttg cttacgcagt ttgtaatcca gcgctgagag cgactcatcc
     3481 agcaacaaca gacgaggctt gttaaccacc gcgcgagcaa tggcgacgcg ttgctgttga
     3541 ccaccagaga gctgatgcgg tttacgttga gcgaaggttt ccagttgcac catccgcagg
     3601 gcttccatca cgcggggcgt aatttcagca gcgggggttt tttgcatgcg caacccaaag
     3661 gccacatttt cgaacacggt catgtggggg aaaagtgcgt agctttggaa aacagtgttc
     3721 acatagcggt tttccgccgg aacgtgggtg atgtcctcgt tatccagcat gatgcgtccg
     3781 gaatcaacag tttccagacc tgcaatcagg cgaagaacgg ttgttttacc gcagccagaa
     3841 gggccaagca gcgtgaggaa ctcgccattg ttgatagtca gatccagctg gggaatgacc
     3901 tctttaccat caaagcattt gcgaattccc gccaattgca ccagcggtga aagcgaactc
     3961 ggttgtttat tcaatttttt actctgtccc atgtaaacgc aacggatggc ttaccgatgc
     4021 ggggtttgtg gttaaccacc ttggtgactc ttaatgaggg cggtaattct acggcaaacc
     4081 gcttgaatcg ccaatctttg ttgtgaatta ctggcttagc tttatattca ttaaggtaat
     4141 gctgataaat attcccgctt gcaggggtaa aagtgacctg acgcaatatt tgtcttttct
     4201 tgcttcttaa taatgttgtc acaaaaagtg agggtgacta catggataaa ctacttgagc
     4261 gatttttgaa ctacgtgtct ctggataccc aatcaaaagc aggggtgaga caggttccca
     4321 gcacggaagg ccaatggaag ttattgcatc tgctgaaaga gcagctcgaa gagatggggc
     4381 ttatcaatgt gaccttaagt gagaagggca ctttgatggc gacgttaccg gctaacgtcc
     4441 ctggcgatat cccggcgatt ggctttattt ctcatgtgga tacctcaccg gattgcagcg
     4501 gcaaaaatgt gaatccgcaa attgttgaaa actatcgcgg tggcgatatt gcgctgggta
     4561 tcggcgatga agttttatca ccggttatgt tcccggtgct gcatcagcta ctgggtcaga
     4621 cgttgattac caccgatggt aaaaccttgt taggtgccga tgacaaagca ggtattgcag
     4681 aaatcatgac cgcgctggcg gtattgcaac agaaaaacat tccgcatggt gatattcgcg
     4741 tcgcctttac cccggatgaa gaagtgggca aaggggcgaa acattttgat gttgatgcct
     4801 tcgatgcccg ctgggcttac actgttgacg gtggtggcgt aggcgaactg gagtttgaaa
     4861 acttcaacgc cgcatcggtc aatatcaaaa ttgtcggtaa caatgttcat ccgggcacgg
     4921 cgaaaggagt gatggtaaat gcgctgtcgc tggcggcacg tattcatgcg gaagttccgg
     4981 cggatgaaag cccggaaatg acagaaggct atgaaggttt ctatcacctg gcgagcatga
     5041 aaggcaccgt tgaccgagcc gatatgcact acatcatccg tgatttcgac cgtaaacagt
     5101 ttgaagcgcg taaacgtaaa atgatggaga tcgccaaaaa agtgggcaaa gggttacatc
     5161 ctgattgcta cattgaactg gtgattgaag acagttacta caatatgcgc gagaaagtgg
     5221 ttgagcatcc gcatattatc gatatcgccc agcaggcgat gcgtgactgc gatattgaac
     5281 cggaactgaa accgatccgc ggcggtaccg acggcgcgca gttgtcgttt atgggattac
     5341 cgtgcccgaa cctgttcact ggcggttaca actatcatgg taagcatgag tttgtgactc
     5401 tggaaggtat ggaaaaagcg gtgcaggtga ttgtccgtat tgccgagtta acggcgcaac
     5461 ggaagtcgtg attgcccgat gcgtatgtgg actgccaatt accgggatga aaccgttata
     5521 ttgatactgg tccacatact tatcgtgcct acaggcgttc agtgcatgta ggcacgataa
     5581 ggcgcaatca gcgccgcatc cggcttaaat ccaaacttac ccttcgaaga accaataccc
     5641 gctattgacc agcgccgcga gcatcgcgag gaatgacgga tcttccagcg catcgccaaa
     5701 attctccgca gtcagcgcaa tgttgctggc gagtgcatcc agtgccggac ggtgcgggga
     5761 atcgatcttc tcaccattgg catacacgtc gtcgccaatg cgcaatacgc gcagaccacc
     5821 caggcgcacc agcacttcac cttgtttcag cgcatcgtag atttcatctg gctgataagg
     5881 cggttccggc ggcgcgatat ccagttcatg acgtgactgg gatataaact cgccaaacca
     5941 ttgcttaaag tgttccggct ggttgatcaa ttcgagcatc atctcacgca gtttatccat
     6001 ctcttgcggc agaacgtccg caggatgagc gcgaggtgga acatccggat cgctgtagta
     6061 gttgccgccc agttcacgtt gcagcacata atcggcaaat ccgctaatta attcccgcgt
     6121 atttggcgcg cgaaaaccca cggaatagtt catcgcattt tccagcgcgt agccttcatg
     6181 cgggaatcct ggcggaatat aaagaatatc gccaggctcc agctcttcat cgatgatggc
     6241 ttcgaacgga tcgacctgta acagatccgg gtgcggacag tgctgtttca tttgcagctt
     6301 ttcgcccact cgccagcgac gacgaccggt accctgaatg ataaacacgt cgtactgatc
     6361 gagatgcggg ccgacgccgc cgcccggtac agaaaaagag atcatcagat catcaatacg
     6421 ccagtccggt agttcacgga acggtcgcat cagcgcggcg gtcggctcat gccagtggtt
     6481 cactgcctgt accagtaatg accagttggt ttcaccgaga tgatcgtagc tttcgaacgg
     6541 gccgtggctg acctgccatt tgccatcctg gtgactgacc agtcgactgt caacttcgct
     6601 ttccatcgcc agacccgcca actcgtctgg agagatcggg tcaataaaat tattaaagcc
     6661 gcgttttaac accaccgggc gtttctgcca gtgacgttca agaaaatcgg gccagttaag
     6721 agtgagttgg tattccatgt taagcatcca caggctggta tctgcaaccg attataacgg
     6781 atgcttaacg taatgcgtga agtatggaca tattttatca attagtgttt tttgccgttt
     6841 cataatgttt cagattgttc actatgtttt tgattat
//
LOCUS       DAZKHF020000230         6856 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2201S31241
            SAMN43267800-rid25106413.denovo.232, whole genome shotgun sequence.
ACCESSION   DAZKHF020000230 DAZKHF020000000
VERSION     DAZKHF020000230.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267800
            Sequence Read Archive: SRR30307263
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6856)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6856)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 6856)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350581.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350581.2
            Genome Coverage       :: 151x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:21:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,652
            CDSs (total)                      :: 5,557
            Genes (coding)                    :: 5,320
            CDSs (with protein)               :: 5,320
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 237
            CDSs (without protein)            :: 237
            Pseudo Genes (ambiguous residues) :: 0 of 237
            Pseudo Genes (frameshifted)       :: 102 of 237
            Pseudo Genes (incomplete)         :: 130 of 237
            Pseudo Genes (internal stop)      :: 52 of 237
            Pseudo Genes (multiple problems)  :: 41 of 237
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6856
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267800-rid25106413.denovo.232"
                     /serotype="O 26:H11"
                     /isolate="2201S31241"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-01"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            98..583
                     /locus_tag="ACHOSC_RS19235"
                     /old_locus_tag="pgaptmp_003847"
     CDS             98..583
                     /locus_tag="ACHOSC_RS19235"
                     /old_locus_tag="pgaptmp_003847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antirestriction protein"
                     /protein_id="HGU4549694.1"
                     /translation="MKTISHNSTTSSVSVTAASGNDQPQLVATIVPDEQRISFWPQHF
                     GLIPQWVTLEPRVFGWMDRLCEDYCGGIWNLYTLNNGGAFMAPEPDDDDDETWVLFNA
                     MNGNRAEMSPEAAGIAACLMTYSHHACRTECYAMTVHYYRLRDYALQHPECSAIMRII
                     D"
     gene            599..1075
                     /gene="radC"
                     /locus_tag="ACHOSC_RS19240"
                     /old_locus_tag="pgaptmp_003848"
     CDS             599..1075
                     /gene="radC"
                     /locus_tag="ACHOSC_RS19240"
                     /old_locus_tag="pgaptmp_003848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadC"
                     /protein_id="HGU4549695.1"
                     /translation="MQQLSFLPGEMTPGERSLILRALKTLDRHLHEPGVAFTSTRAAR
                     EWLILNMAGLEREEFRVLYLNNQNQLIAGETLFTGTINRTEVHPREVIKRALYHNAAA
                     VVLAHNHPSGEVTPSKADRLITERLVQALALVDIRVPDHLIVGGSQVFSFAEHGLL"
     gene            1144..1365
                     /gene="yeeT"
                     /locus_tag="ACHOSC_RS19245"
                     /old_locus_tag="pgaptmp_003849"
     CDS             1144..1365
                     /gene="yeeT"
                     /locus_tag="ACHOSC_RS19245"
                     /old_locus_tag="pgaptmp_003849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF987 domain-containing protein"
                     /protein_id="HGU4549696.1"
                     /translation="MKIITRGEAMRIHQQHPTSRLFPFCTGKYRWHGSAEAYTGREVQ
                     DIPGVLAVFAERRKDSFGPYVRLMSVTLN"
     gene            1528..1896
                     /locus_tag="ACHOSC_RS19250"
                     /old_locus_tag="pgaptmp_003850"
     CDS             1528..1896
                     /locus_tag="ACHOSC_RS19250"
                     /old_locus_tag="pgaptmp_003850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001280950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0051495 - positive regulation of
                     cytoskeleton organization [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type IV toxin-antitoxin system YeeU family
                     antitoxin"
                     /protein_id="HGU4549697.1"
                     /translation="MSDTLSGTTHPDDNDDHPWWGLPCTVTPCFGARLVQEGNRLHYL
                     ADRAGIRGRFRDADAYPLDQAFPLLMKQLKLMLTSGELNPRHQHTVTLYAKGLTCEAD
                     TLGSCGYVYLAVYPTPETKK"
     gene            1986..2363
                     /locus_tag="ACHOSC_RS19255"
                     /old_locus_tag="pgaptmp_003851"
     CDS             1986..2363
                     /locus_tag="ACHOSC_RS19255"
                     /old_locus_tag="pgaptmp_003851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020239812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TA system toxin CbtA family protein"
                     /protein_id="HGU4549698.1"
                     /translation="MKTLPDTHVREVSCCPSLVTIWQTLLTRLLDQHYGLTLNDTPFA
                     DERVIEQHIEAGISLCDAVNFLVEKYALVRTDQSGFSACTHSQLINSIDILRARRATG
                     LMTRDNYRTVNNITLGKHPEAKR"
     gene            2360..2509
                     /locus_tag="ACHOSC_RS19260"
                     /old_locus_tag="pgaptmp_003852"
                     /pseudo
     CDS             2360..2509
                     /locus_tag="ACHOSC_RS19260"
                     /old_locus_tag="pgaptmp_003852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000761660.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5983 family protein"
     gene            2609..2689
                     /locus_tag="ACHOSC_RS19265"
                     /old_locus_tag="pgaptmp_003853"
                     /pseudo
     CDS             2609..2689
                     /locus_tag="ACHOSC_RS19265"
                     /old_locus_tag="pgaptmp_003853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309435.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            3505..3876
                     /locus_tag="ACHOSC_RS19270"
                     /old_locus_tag="pgaptmp_003854"
     CDS             3505..3876
                     /locus_tag="ACHOSC_RS19270"
                     /old_locus_tag="pgaptmp_003854"
                     /inference="COORDINATES: protein motif:HMM:NF014430.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS110 family transposase"
                     /protein_id="HGU4549699.1"
                     /translation="MLFLPPLQLSAPGSIGFKNRRQLAAWLGLVPRQCFSVGKNTLGS
                     ISKRDDVYIRTLLFHGAKTVSNARKGRQVQESWLGRPVSRRNKNVATVALNNKNARVL
                     WVLLTNDKEISPEEVMVAAYM"
     gene            4147..4512
                     /locus_tag="ACHOSC_RS19275"
                     /old_locus_tag="pgaptmp_003855"
     CDS             4147..4512
                     /locus_tag="ACHOSC_RS19275"
                     /old_locus_tag="pgaptmp_003855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001282181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006576 - biogenic amine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EutP/PduV family microcompartment system
                     protein"
                     /protein_id="HGU4549700.1"
                     /translation="MSDMTISLPKEVNGHVICSTRIRHILAIRKSILMQIYMLFRQQA
                     GISALRQELEVKTPYRAMNHPVIGVVTKADLASMEHISLVKCWLREAGAHNVLVTSAV
                     NNNGVTELFALLHTEEGCC"
     gene            complement(4613..4942)
                     /gene="tmaR"
                     /locus_tag="ACHOSC_RS19280"
                     /old_locus_tag="pgaptmp_003856"
     CDS             complement(4613..4942)
                     /gene="tmaR"
                     /locus_tag="ACHOSC_RS19280"
                     /old_locus_tag="pgaptmp_003856"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310836.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF496 family protein"
                     /protein_id="HGU4549701.1"
                     /translation="METTKPSFQDVLEFVRLFRRKNKLQREIQDVEKKIRDNQKRVLL
                     LDNLSDYIKPGMSVEAIQGIIASMKGDYEDRVDDYIIKNAELSKERRDISKKLKAMGE
                     MKNGEAK"
     gene            complement(5114..6172)
                     /gene="yeeA"
                     /locus_tag="ACHOSC_RS19285"
                     /old_locus_tag="pgaptmp_003857"
     CDS             complement(5114..6172)
                     /gene="yeeA"
                     /locus_tag="ACHOSC_RS19285"
                     /old_locus_tag="pgaptmp_003857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HGU4549702.1"
                     /translation="MRADKSLSPFEIRVYRHYRIVHGTRVALAFLLTFLIIRLFTIPE
                     STWPLVTMVVIMGPISFWGNVVPRAFERIGGTVLGSILGLIALQLELISLPLMLVWCA
                     AAMFLCGWLALGKKPYQGLLIGVTLAIVVGSPTGEIDTALWRSGDVILGSLLAMLFTG
                     IWPQRAFIHWRIQLAKSLTEYNRVYQSAFSPNLLERPRLESHLQKLLTDAVKMRGLIA
                     PASKETRIPKSIYEGIQTINRHLVCMLELQINAYWATRPSHFVLLNAQKLRDTQHMMQ
                     QILLSLVHALYEGNPQPVFANTEKLIDAVEELRQLLNNHHDLKVVETPIYGYVWLNME
                     TAHQLELLSSLICRALRK"
     gene            complement(6370..6843)
                     /gene="sbmC"
                     /locus_tag="ACHOSC_RS19290"
                     /old_locus_tag="pgaptmp_003858"
     CDS             complement(6370..6843)
                     /gene="sbmC"
                     /locus_tag="ACHOSC_RS19290"
                     /old_locus_tag="pgaptmp_003858"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase inhibitor SbmC"
                     /protein_id="HGU4549703.1"
                     /translation="MNYEIKQEDKRTVAGFHLVGPWEQTVKKGFEQLMMWVDSKNIVP
                     KEWVAVFYDNPDETPAEKLRCDIVVTVPNNFTLPENSEGVILTEISGGQYAVAVARVV
                     GDDFAKPWYQFFNSLLQDSAYEMLPKPCFEVYLNNGAEDGYWDIEMYVAVQPKHH"
BASE COUNT         1790 a         1766 c         1649 g         1651 t
ORIGIN      
        1 attgtgggtg atggcggaaa ccctgctgga gagcctgcgc tgattcagcc attaccctga
       61 aaaccacgca tttatttgtc ctttaaggag tcatttcatg aaaaccattt cacataattc
      121 caccacatct tctgtttccg taaccgctgc atcagggaat gaccagcctc agttggttgc
      181 caccatagtc cctgatgaac agcgcatcag cttctggccg cagcattttg gcctcattcc
      241 acagtgggtg accctggagc cccgtgtttt cggctggatg gaccgtctgt gcgaagacta
      301 ctgcggtggt atctggaatc tgtacactct gaacaacggc ggggcattta tggcccccga
      361 accggatgac gatgatgacg aaacatgggt actgttcaat gccatgaacg gcaaccgcgc
      421 tgaaatgagc ccggaagccg ctggcattgc tgcctgtctg atgacgtaca gtcatcatgc
      481 ctgtcgtacg gagtgttatg ccatgacggt ccattattac cggctgcggg attacgccct
      541 gcagcatccg gaatgcagcg ccattatgcg catcatcgac tgaatggagg cagggacaat
      601 gcagcagctt tcctttctgc ccggagaaat gacgcccggc gagcgcagtc tcattctgcg
      661 ggctctgaaa accctggacc gccatcttca tgaacccggt gtggccttca cctccacccg
      721 tgcggcacgg gaatggctga ttctgaacat ggcgggactg gagcgtgaag aattccgggt
      781 gctgtatctg aataaccaga atcagctgat tgccggtgaa accctcttca ccggcaccat
      841 caaccgcacg gaagtccatc cccgggaagt gattaaacgt gccctgtatc acaatgccgc
      901 agccgtggtg ctggcacaca atcacccgtc cggtgaagtc acccccagta aggcagaccg
      961 ccttatcacg gaacgtctgg tacaggcgct ggccctggtt gatatccggg tgccggacca
     1021 tctgatagtc ggtggcagcc aggttttctc ctttgcggaa cacggtctgc tttaacccgt
     1081 caccgtcaca accacatcac acctgttttc acttttatct tctgtcttca gaggtatcac
     1141 actatgaaaa tcatcacccg tggtgaagcc atgcgtattc accaacaaca tccgacatcc
     1201 cgtctttttc cgttctgtac cggcaaatac cgctggcacg gcagtgctga agcgtatacc
     1261 ggtcgtgaag tgcaggatat tcccggtgtg ctggccgtgt ttgcagaacg ccgtaaggac
     1321 agttttggtc cgtatgtccg gctgatgagc gtcaccctga actgatgttc cgtcacccgg
     1381 aaaacgagta cgactggcag agctcagacg ccgctgtgga tgaggtcctg ctcatcaacg
     1441 aatacacctt taccgaagcc ggtctgcgcg ccggctgacc cgtcatcgtt aaactatcat
     1501 tcaccgattt tctgtgagga ttttatcgtg tcagacacac tttccgggac aacgcatccc
     1561 gatgacaatg acgaccatcc ctggtgggga ctaccctgca cagtgacacc ctgtttcggc
     1621 gcccgtctgg tgcaggaggg taaccggttg cattaccttg ccgaccgtgc cggtatcaga
     1681 ggccggttca gagatgcgga tgcgtaccct ctggaccagg cctttccgct gctgatgaaa
     1741 caactgaaat taatgctcac cagtggtgaa ctgaatcccc gccatcagca taccgtgaca
     1801 ctgtatgcga aggggctgac ctgtgaagcc gacaccctcg gctcctgtgg ttacgtttat
     1861 ctggctgttt atccgacacc tgaaacgaaa aagtaaacca ccggaataac cctctactcc
     1921 ggcctggtgt tttcaccacg ccactgtccc attttttatc tctgcatatc aggaaaatct
     1981 tcagtatgaa aacattaccc gacacgcatg tacgggaggt atcgtgctgc ccgtctctcg
     2041 tcaccatctg gcagacactg ctcacccgac tgctggacca gcactacggc cttacgctga
     2101 atgacacacc gttcgctgat gaacgtgtga ttgaacagca tattgaggca ggcatttcac
     2161 tgtgtgacgc ggtgaacttt ctcgttgaaa aatacgcgct ggtacgtacc gaccagtcgg
     2221 gattcagcgc ctgtacccac tctcagttaa taaacagcat cgatatcctc cgggctcgcc
     2281 gggcaacagg cctgatgacc cgcgacaatt acagaacggt aaataacatt accctgggga
     2341 agcatccgga ggcgaaacga tgaaactggc cctgacgctg gaagccgaca gcgttaacgt
     2401 gcaggcgctg aacatggggc gcattgtcgt tgacgtcgat ggtgttaatc tcgctgaact
     2461 gattaacaag gtcgctgaaa acggtgattc actccgcgtg gtggatgaac gtgactctac
     2521 cgaaacaccg gcaacttatg ccagccctca ccagttgctg taagacaatg cagtgataca
     2581 gggtatagtg ccattaacca cagaaacagc actgttgtcc tgtattaagc aggcaagaga
     2641 agatgtcctt accctgcgcc atctgcagct tctgcaccag aaccggtgaa tctcactcaa
     2701 caggcttcat ttcccctgac gccagcctga atacagctgg cgttttcatt tataaacaga
     2761 aaggaaaacc gataataatg gaatggacgc ccctaccatg aagcaacggt aggatattac
     2821 tgataaacct tccagtggag gtgcgttatg gccgttttaa cgattagcat tgacctgaca
     2881 aaaaatgaat tccaaatcca cggtctgggt aggaacagaa aaatctaagc tcagaaaacg
     2941 gattaaacgt tccctgatgc ccgtattctt cgcgaatatg tcttcctgtc ggattgggat
     3001 ggaggtctgc gccggagcgc gtcattagtg agaatgctgt ctacttaggg gcataatgtc
     3061 agactaatgc ccgcgatttg taaagccata tgtgaaaaca aacaagaatg atatggctgc
     3121 tgccgaggcc atctgtgaag ccgttacccg acctaatatg cggtttgtca gcatcaagac
     3181 gcctgagcag tagtcattac tgtcttttca tcgtgcccaa acgggatttg ttaaatcaag
     3241 ggctgcgcag ataaaccaga tcagagaatt actggctgaa tttggcatcg ttttcccgcc
     3301 cgacattgta gcattcagtc gtcatgtccc tgccctgcct gaagacgcgg aaaactgttt
     3361 aacaatgccg ttcagacagc tgtcgtccta tctctaatga aaatgtgaaa caactgagcg
     3421 tacacattga aaccatagaa gccgtgagca acgaacacgt cgggaacgac gaactgtgta
     3481 ggaaaattgc caccatttcg gggattgcta ttcttaccac cactgcaatt gtcagcaccg
     3541 ggtagtatcg gcttcaaaaa ccggcgtcag cttgctgcat ggcttggact ggttccccga
     3601 cagtgcttca gcgtaggtaa aaacacgctg gggtctatca gtaaacggga cgatgtctat
     3661 atcagaacgc tactgttcca tggtgcgaag acagtgtcaa acgccagaaa gggcagacag
     3721 gtccaggaaa gttggctggg cagaccagtc tcgcggcgga ataaaaatgt tgccaccgta
     3781 gcgctgaaca acaaaaatgc cagagtgctc tgggtattgc taaccaacga taaagagatt
     3841 tcaccagaag aagtgatggt agctgcatat atgtaaatga aactacttct gtctgttgaa
     3901 aaataaaacc aacctactga ttgctaagga taaacaagta ttaaaggcca gactgataag
     3961 accgcgacta agtgaacctg tatctgacat ggcatagctt cgctaatgtg tcatcatgat
     4021 taggactaaa tctgtgaatt cctttaggaa gcacggacac aggccaatct gagtccatat
     4081 gtatgattgg aatcttacac agatgacttt ttgacacggg gacttggcaa acaggggcga
     4141 ccatgtatgt cagatatgac aatctcactt ccgaaagaag tcaacgggca cgttatctgt
     4201 tcaaccagaa tccgacacat tctggctata cgaaagagca tacttatgca aatctatatg
     4261 cttttccggc aacaagcagg aatctcagcg ctgaggcagg aattagaggt aaaaacgccc
     4321 taccgggcca tgaatcaccc ggtcattgga gtagtaacca aagcagattt agccagcatg
     4381 gaacacatat ctttggtgaa atgttggtta cgggaagctg gagcgcacaa cgtgttagta
     4441 accagtgcgg ttaataacaa tggagttaca gaactttttg ccttgctgca tacagaagaa
     4501 ggctgttgct aatttcgata ggttctcaga tgagaatgat gacggtgata cacagataag
     4561 aatcaatgag caatgagggt tgccgggcaa ccctcattga ataaaacagg aattacttcg
     4621 cttcgccgtt tttcatttcg cccatagctt tcagcttttt ggagatatcg cggcgttctt
     4681 tggagagctc ggcatttttg atgatgtaat cgtcaacgcg atcttcatag tcacctttca
     4741 tgctggcgat gatgccctgg attgcttcaa cgctcatccc gggcttgatg taatcgctca
     4801 ggttgtccag caacaggacg cgcttctggt tgtcacggat ctttttctca acgtcctgaa
     4861 tttcacgttg cagtttgttc ttacgacgga acagacgaac aaattccagt acgtcctgga
     4921 atgaaggctt ggtagtttcc atttttatac ccctgataat gtgagagtcg gattcgttta
     4981 agcaaccgct attcgttagg gccaacatta ctcatagctg ccgcagatga caatgctttt
     5041 atcccttttc actatcatac cctttatcct tgctgaatcg aagcagcagc aagatgattc
     5101 tgaagttcag gaattatttg cgcaaggccc ggcaaatcag actcgatagc aactcaagct
     5161 gatgtgccgt ttccatgttc agccacacat aaccatagat tggtgtttcc acaaccttca
     5221 ggtcatggtg gttattcagc aactgacgca gctcttccac agcatcgatc aatttttccg
     5281 tattggcaaa aaccggctgc ggattacctt cgtacagcgc atgaacaagg ctcagcagta
     5341 tttgctgcat catgtgctgg gtatcacgaa gtttttgcgc gttcaataac acgaaatggc
     5401 tggggcgcgt ggcccagtat gcattgattt gcaactccag catacaaacc agatggcggt
     5461 taatggtctg gataccttca tatatcgatt ttggaatacg agtttctttg ctggcgggcg
     5521 caatcagccc acgcattttc acggcatcgg tcaggagttt ttgtagatgg ctttccagac
     5581 gtgggcgttc gagtaagttc ggtgagaatg cagattgata aacccgatta tactcggtca
     5641 gacttttcgc cagttgaatg cgccagtgga tgaacgcccg ttgtggccag ataccggtaa
     5701 acaacattgc cagtaaagag ccgaggatca catcgccgct tcgccataac gccgtatcaa
     5761 tttcacctgt cggggaaccc acaacaattg ccagcgtcac cccaatcaat aatccttgat
     5821 acggtttctt gcccagcgcc agccaaccgc aaagaaacat agccgccgcg caccagacta
     5881 acatcagcgg taacgaaatt aactccagtt gcagagcgat aagacctaaa atcgaaccca
     5941 acaccgtacc gccaatacgt tcgaaggcgc gagggacaac gttaccccag aacgagattg
     6001 gccccataat caccaccatg gtgaccagcg gccaggtgct ttccgggata gtaaacaggc
     6061 ggataatgag aaaagtgagc aggaatgcca gcgcgacccg agtaccatgc acaatgcggt
     6121 aatggcggta tacccggatt tcaaacgggc ttaatgactt atcggcacgc acgcagaaac
     6181 tccgtaatga agattaaaag tgctcataac tcatctgtcg gccagaaggc ccgcctctga
     6241 attatccgac ggacaaaccg ggaacagtgt aatttcccgg caggagcgaa aaaattgaaa
     6301 ggcgcatctt actcttttcg ctttcaaaaa aagagtggtc atcgcgttaa cacaccgccc
     6361 tgagatgaat tagtgatgtt ttggctgcac cgcaacatac atttcgatat cccagtaccc
     6421 atcttccgcg ccattgttca aataaacctc gaagcagggc tttggtaaca tttcataagc
     6481 actgtcctgc aagaggctat taaagaactg ataccagggt ttagcaaaat catcaccgac
     6541 tacacgagcc accgcaaccg catactgacc acctgaaatt tctgtcagaa tgacgccctc
     6601 actgttttcg gggagcgtaa agttattcgg caccgtcacg acgatgtcgc agcgtaattt
     6661 ttcggcgggt gtttcatctg gattgtcgta aaagacagca acccactcct tcggcacaat
     6721 atttttgcta tctacccaca tcatcaactg ctcaaagcct ttctttaccg tctgttccca
     6781 cgggccaacg agatggaaac ctgcaacggt acgtttatct tcctgcttaa tctcgtagtt
     6841 catgacgtcc cattaa
//
LOCUS       DAZKHF020000231         6806 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2201S31241
            SAMN43267800-rid25106413.denovo.233, whole genome shotgun sequence.
ACCESSION   DAZKHF020000231 DAZKHF020000000
VERSION     DAZKHF020000231.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267800
            Sequence Read Archive: SRR30307263
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6806)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6806)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 6806)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350581.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350581.2
            Genome Coverage       :: 151x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:21:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,652
            CDSs (total)                      :: 5,557
            Genes (coding)                    :: 5,320
            CDSs (with protein)               :: 5,320
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 237
            CDSs (without protein)            :: 237
            Pseudo Genes (ambiguous residues) :: 0 of 237
            Pseudo Genes (frameshifted)       :: 102 of 237
            Pseudo Genes (incomplete)         :: 130 of 237
            Pseudo Genes (internal stop)      :: 52 of 237
            Pseudo Genes (multiple problems)  :: 41 of 237
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6806
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267800-rid25106413.denovo.233"
                     /serotype="O 26:H11"
                     /isolate="2201S31241"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-01"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            complement(127..795)
                     /gene="artM"
                     /locus_tag="ACHOSC_RS19295"
                     /old_locus_tag="pgaptmp_003859"
     CDS             complement(127..795)
                     /gene="artM"
                     /locus_tag="ACHOSC_RS19295"
                     /old_locus_tag="pgaptmp_003859"
                     /EC_number="7.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0071705 - nitrogen compound transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine ABC transporter permease ArtM"
                     /protein_id="HGU4549704.1"
                     /translation="MFEYLPELMKGLHTSLTLTVASLIVALILALIFTIILTLKTPVL
                     VWLVRGYITLFTGTPLLVQIFLIYYGPGQFPTLQEYPALWHLLSEPWLCALIALSLNS
                     AAYTTQLFYGAIRAIPEGQWQSCSALGMSKKDTLAILLPYAFKRSLSSYSNEVVLVFK
                     STSLAYTITLMEVMGYSQLLYGRTYDVMVFGAAGIIYLVVNGLLTLMMRLIERKALAF
                     ERRN"
     gene            complement(795..1511)
                     /gene="artQ"
                     /locus_tag="ACHOSC_RS19300"
                     /old_locus_tag="pgaptmp_003860"
     CDS             complement(795..1511)
                     /gene="artQ"
                     /locus_tag="ACHOSC_RS19300"
                     /old_locus_tag="pgaptmp_003860"
                     /EC_number="7.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA];
                     GO_process: GO:0071705 - nitrogen compound transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine ABC transporter permease ArtQ"
                     /protein_id="HGU4549705.1"
                     /translation="MNEFFPLASAAGMTVGLAVCALIVGLALAMFFAVWESAKWRPVA
                     WAGSALVTILRGLPEILVVLFIYFGSSQLLLTLSDGFTINLGFVQIPVQMDIENFDVS
                     PFLCGVIALSLLYAAYASQTLRGALKAVPVGQWESGQALGLSKSAIFFRLVMPQMWRH
                     ALPGLGNQWLVLLKDTALVSLISVNDLMLQTKSIATRTQEPFTWYIVAAAIYLVITLL
                     SQYILKRIDLRATRFERRPS"
     gene            complement(1518..2249)
                     /gene="artI"
                     /locus_tag="ACHOSC_RS19305"
                     /old_locus_tag="pgaptmp_003861"
     CDS             complement(1518..2249)
                     /gene="artI"
                     /locus_tag="ACHOSC_RS19305"
                     /old_locus_tag="pgaptmp_003861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0015424 - ABC-type amino acid transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine ABC transporter substrate-binding
                     protein ArtI"
                     /protein_id="HGU4549706.1"
                     /translation="MKKVLIAALIAGFSLSATAAETIRFATEASYPPFESIDANNQIV
                     GFDVDLAQALCKEIDATCTFSNQAFDSLIPSLKFRRVEAVMAGMDITPEREKQVLFTT
                     PYYDNSALFVGQQGKYTSVDQLKGKKVGVQNGTTHQKFIMDKHPEITTVPYDSYQNAK
                     LDLQNGRIDGVFGDTAVVTEWLKDNPKLAAVGDKVTDKDYFGTGLGIAVRQGNTELQQ
                     KLNTALEKVKKDGTYETIYNKWFQK"
     gene            complement(2267..2995)
                     /gene="artP"
                     /locus_tag="ACHOSC_RS19310"
                     /old_locus_tag="pgaptmp_003862"
     CDS             complement(2267..2995)
                     /gene="artP"
                     /locus_tag="ACHOSC_RS19310"
                     /old_locus_tag="pgaptmp_003862"
                     /EC_number="7.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001742668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015424 - ABC-type amino acid transporter
                     activity [Evidence IEA];
                     GO_process: GO:0003333 - amino acid transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine ABC transporter ATP-binding protein
                     ArtP"
                     /protein_id="HGU4549707.1"
                     /translation="MSIQLNGINCFYGAHQALFDITLDCPQGETLVLLGPSGAGKSSL
                     LRVLNLLEMPRSGTLNIAGNHFDFTKTPSDKAIRDLRRNVGMVFQQYNLWPHLTVQQN
                     LIEAPCRVLGLSKDQALARAEKLLERLRLKPYSDRYPLHLSGGQQQRVAIARALMMEP
                     QVLLFDEPTAALDPEITAQIVSIIRELAETNITQVIVTHEVEVARKTASRVVYMENGH
                     IVEQGDASCFTEPQTEAFKNYLSH"
     gene            complement(3213..3728)
                     /gene="ybjP"
                     /locus_tag="ACHOSC_RS19315"
                     /old_locus_tag="pgaptmp_003863"
     CDS             complement(3213..3728)
                     /gene="ybjP"
                     /locus_tag="ACHOSC_RS19315"
                     /old_locus_tag="pgaptmp_003863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HGU4549708.1"
                     /translation="MRYSKLTMLIPCALLLSACTTVTPAYKDNGTRSGPCVEGGPDNV
                     AQQFYDYRILHRSNDITALRPYLSDKLATLLSDASRDNNHRELLTNDPFSSRTTLPES
                     ARVASASTIPNRDARNIPLRVDLKQGDQSWQDEVLMIQEGQCWVIDDVRYLGGSVHAT
                     AGTLRQSIENR"
     gene            3854..4177
                     /gene="ybjQ"
                     /locus_tag="ACHOSC_RS19320"
                     /old_locus_tag="pgaptmp_003864"
     CDS             3854..4177
                     /gene="ybjQ"
                     /locus_tag="ACHOSC_RS19320"
                     /old_locus_tag="pgaptmp_003864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal-binding domain-containing protein"
                     /protein_id="HGU4549709.1"
                     /translation="MQFSTTPTLEGQIIVEYCGVVTGEAILGANIFRDFFAGIRDIVG
                     GRSGAYEKELRKAREIAFEELGSQARALGADAVVGIDIDYETVGQNGSMLMVSVSGTA
                     VKTRR"
     gene            4174..5004
                     /gene="amiD"
                     /locus_tag="ACHOSC_RS19325"
                     /old_locus_tag="pgaptmp_003865"
     CDS             4174..5004
                     /gene="amiD"
                     /locus_tag="ACHOSC_RS19325"
                     /old_locus_tag="pgaptmp_003865"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase AmiD"
                     /protein_id="HGU4549710.1"
                     /translation="MRRVFWLIAAALLLAGCAGEKGIVEKEGYQLDTRRQAQAAYPRI
                     KVLVIHYTADDFDSSLATLTDKQVSSHYLVPAVPPRYNGKPRIWQLVPEQELAWHAGI
                     SAWRGATRLNDTSIGIELENRGWQKSAGVKYFAPFEPAQIQALIPLAKDIIARYHIKP
                     ENVVAHADIAPQRKDDPGPLFPWQQLAQQGIGAWPDAQRVNFYLAGRAPHTPVDTASL
                     LELLARYGYDVKPDMTPREQRRVIMAFQMHFRPTLYNGEADAETQAIAEALLEKYGQD
                     "
     gene            complement(5001..6014)
                     /gene="ybjS"
                     /locus_tag="ACHOSC_RS19330"
                     /old_locus_tag="pgaptmp_003866"
     CDS             complement(5001..6014)
                     /gene="ybjS"
                     /locus_tag="ACHOSC_RS19330"
                     /old_locus_tag="pgaptmp_003866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415389.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HGU4549711.1"
                     /translation="MKVLVTGATSGLGRNAVEFLCQKGISVRATGRNEAMGKLLEKMG
                     AEFVPADLTELVSSQAKVMLAGIDTLWHCSSFTSPWGTQQAFDLANVRATRRLGEWTV
                     AWGVRNFIHISSPSLYFDYHHHRDIKEDFRPHRFANEFARSKAASEEVINMLSQANPQ
                     TRFTILRPQSLFGPHDKVFIPRLAHMMHHYGSILLPHGGSALVDMTYYENAVHAMWLA
                     SQEACDKLPSGRVYNITNGEHRTLRSIVQKLIDELNIDCRIRSVPYPMLDMIARSMER
                     LGRKSAKEPPLTHYGVSKLNFDFTLDITRAQEELGYQPVITLDEGIEKTAAWLRDHGK
                     LPR"
     gene            complement(6113..>6806)
                     /locus_tag="ACHOSC_RS19335"
                     /old_locus_tag="pgaptmp_003867"
     CDS             complement(6113..>6806)
                     /locus_tag="ACHOSC_RS19335"
                     /old_locus_tag="pgaptmp_003867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415390.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF2867 domain-containing protein"
                     /protein_id="HGU4549712.1"
                     /translation="LNVITSVPPTTARALIQGLKHDLLADDTALRALIPQRLIAFDDA
                     VRSTLKEEEKLVNSSDWGYDAQAFARWRPEYGYFAKQAGFTVKTSASLAALWQVVNQI
                     GGKERYFFGNILWQTRALMDRAIGHKLAKGRPEREYLQTGDAVDSWKVIVVEPEKQLT
                     LLFGMKAPGLGRLCFSLEDKGDYSTIDVRAFWHPHGMPGLFYWLLMIPAHLFIFRGMA
                     KQIARLAEQSTD"
BASE COUNT         1643 a         1789 c         1799 g         1575 t
ORIGIN      
        1 atgcactatg taggccggat aaggcgtcca cgccgcatcc ggcacaggca ccgtgctgat
       61 gtctgatgcg acgctggcgc gtcttatcag acctacaaac ccccccggcg aatgtacgca
      121 gctacattaa tttcgccgtt cgaatgccag tgctttgcgc tcgatcagac gcatcatcag
      181 cgtcagcagg ccgttgacga ccaggtaaat aatccctgcc gcaccgaaca ccattacatc
      241 gtaggtgcgt ccgtacaaca actggctgta tcccatcact tccatcagcg taatggtgta
      301 agccagagag gtacttttga acaccagcac cacttcgttg gaataagaag agagcgagcg
      361 tttaaaggca tacggcagca ggatcgccag cgtatctttt ttgctcattc ccagggcgct
      421 acaggactgc cactgacctt ctgggatcgc acgaattgca ccgtaaaaca gctgcgtggt
      481 atacgccgca ctgttcagcg ataacgcaat cagcgcacat aaccacggtt ctgacaacaa
      541 atgccacagt gccggatact cctgcaaagt cggaaactgg cccggcccgt aataaatcag
      601 gaagatctgc accagcagcg gcgtaccggt aaacagcgtg atataacccc gcaccagcca
      661 caccagcacc ggcgttttca gcgtcaggat gatggtaaaa atcaatgcca gaatcagtgc
      721 cacaatcagc gaggcaacgg ttagcgtcag gctggtgtgt agccctttca tcagttcggg
      781 taaatactca aacattagct gggcctccgc tcaaaacgtg tcgcgcgcag gtcaatgcgt
      841 ttgagaatgt actgactgag cagggtgatc accaggtaaa tcgccgccgc cacaatgtac
      901 caggtaaatg gttcctgggt acgagtagcg atgctttttg tttgcagcat taaatcattc
      961 acactaatca aactgaccag cgcggtatct ttcagcagca ccagccactg gttaccgagg
     1021 ccaggcagcg catgacgcca catctgcggc atcaccagac ggaaaaagat agccgatttc
     1081 gacagcccca gcgcctgacc agactcccac tgacccaccg gcaccgcttt caacgcgccc
     1141 cgaagcgttt gcgaggcata ggcggcatac agcagtgaca gagcgatgac accacaaagg
     1201 aacgggctaa cgtcgaagtt ctcaatgtcc atctgcactg ggatctgcac gaacccaaga
     1261 ttgatagtga agccatccga aagcgtcagc agcagctgcg aggagccaaa atagataaac
     1321 agcaccacca gaatttctgg caggccacgc agaatggtta ccagcgctga acctgcccac
     1381 gcgacaggac gccattttgc cgactcccat accgcaaaga acatcgccag cgccagcccg
     1441 acaatcaatg cacaaacggc aaggccgacg gtcatcccgg cggcgcttgc taaaggaaaa
     1501 aattcattca tcaggaatta cttctggaac catttgttgt agatggtttc gtaagtgcca
     1561 tctttcttca ctttttccag cgcagtgttg agtttctgct gcagctcagt gttgccctga
     1621 cgtaccgcga tgccgaggcc agtgccgaag taatctttat cggtcacttt gtcgcccacc
     1681 gccgccagtt tcgggttatc tttcagccac tcagtgacca ctgcggtgtc accgaagacg
     1741 ccgtcgatac gcccgttttg cagatccagt tttgcgttct ggtagctgtc atacggaacg
     1801 gtagtgattt ccgggtgctt atccataatg aatttctggt gtgtcgtccc gttctgtacg
     1861 ccgacttttt tgcctttcag ctgatcaaca ctggtgtatt tgccttgctg acccacaaac
     1921 agggcagagt tgtcatagta cggggtggta aacagcacct gcttttcacg ctccggagtg
     1981 atatccatgc ccgccatcac ggcttctacg cgacggaatt tcaggcttgg gatcaggctg
     2041 tcaaacgcct ggttagagaa agtgcaggtt gcatcaatct ctttacacag cgcttgtgcc
     2101 aggtcgacgt caaaaccaac gatctggttg tttgcatcaa tcgattcaaa cggaggatag
     2161 gatgcttcgg tagcaaaacg aatggtttcg gcagctgtgg cggaaagact aaaacctgca
     2221 attaacgcgg caatcagaac ttttttcatt gttgttatcc cgaatcttag tgagagagat
     2281 agtttttaaa tgcttcggtt tgcggctcgg taaagcagct cgcgtcgcct tgttctacga
     2341 tatgaccatt ttccatatac accactcggc tggcggtttt acgcgccact tcaacttcgt
     2401 gggtgacgat cacctgggta atattcgttt ctgccagctc acgaatgatg ctgacgattt
     2461 gtgccgtaat ttccgggtcc agtgcggcgg tcggttcatc gaacagcagt acctgcggtt
     2521 ccatcatcaa cgcacgggca atagcaacac gctgctgctg accaccagaa agatgcagcg
     2581 ggtaacgatc gctataaggt ttgagacgca gacgttccag cagtttttct gcacgggcca
     2641 gcgcctgatc tttactcaac cccagtacac ggcagggcgc ttcaatcagg ttttgctgca
     2701 cggtcagatg cggccacagg ttgtattgct gaaacaccat gccaacgtta cgacgcaaat
     2761 cgcgaatcgc tttgtcagag ggtgttttgg tgaaatcgaa atggttgcct gcaatgttga
     2821 gcgtaccgga gcgcggcatc tcaagcagat tgagtacacg cagcagcgag cttttacccg
     2881 cgccgctggg gccaagtaac accagcgttt cgccctgtgg gcaatccagc gtgatatcga
     2941 acagcgcctg atgcgcgccg tagaagcaat taatgccgtt taattgaata ctcattgaca
     3001 ctcgtatact ggcagtctga tagctattga ggtcgaagat agtacctttg acagaataat
     3061 tatgcaatat ttctgcttta aaagttaaaa gcaaagcgca ttattcaata aacatagcac
     3121 aaaataacgg gggcggtggt cggcgagcat aaatgtcggc attcctcacg aaatgccgga
     3181 caatttacgg ggtttattgg ttgatcaagg cgttagcgat tctcgatgga ctgacggagc
     3241 gtacccgccg tggcatgaac gctgccgcct aaatagcgta catcgtcgat cacccaacac
     3301 tggccttcct gaatcattaa cacttcatcc tgccagctct gatcaccctg ttttagatcc
     3361 acacgcagag gaatatttcg cgcatcacga ttcgggattg ttgaggcact ggcaacacgg
     3421 gcgctctctg gcaaggttgt gcgactggag aatggatcgt tggtcagtag ttcccgatgg
     3481 ttattatccc gggaggcatc gctaagcagt gtcgccagtt tgtcgctcag ataagggcgc
     3541 aaggcggtga tgtcgttgct gcggtgcaaa atgcggtagt cataaaattg ctgggccacg
     3601 ttatccgggc ctccttcaac gcaaggacca ctgcgtgtgc cgttatcttt ataagctgga
     3661 gtgactgtgg tgcaggcact gaggagcagt gcgcagggga taagcattgt caatttgctg
     3721 tagcgcataa tgatttcctt ataagcgatc gctctgaaag cgttctacga taataatgat
     3781 atcctttcaa taatagcgta tcagtctgat aatgctttag agatcgaagg cttaacaaac
     3841 aaggagatcg atcatgcaat tttctacaac cccaactctg gaaggccaga tcatcgttga
     3901 atattgcggt gtggtgaccg gcgaagcgat tttaggtgcc aatattttcc gtgatttctt
     3961 tgccggtatc cgcgatatcg ttggcggacg ttccggtgcg tatgaaaaag aactgcgtaa
     4021 agcacgggag atcgcctttg aggaattagg ctcccaggcg cgggcgctgg gggccgatgc
     4081 cgtcgtcggt attgatatcg actacgaaac ggtcgggcaa aacggcagta tgctgatggt
     4141 tagcgtcagc ggtacggcgg tgaaaacgcg tcgatgagaa gagtcttctg gctgatcgct
     4201 gccgctctgt tactggcagg gtgtgcaggc gaaaaaggca ttgtcgagaa agagggatat
     4261 cagcttgata cccgacgcca ggcgcaggcg gcgtatccgc gcattaaagt gctggtgatc
     4321 cactacaccg cagatgattt tgatagctcg ctggcgacac tgaccgataa gcaggtcagc
     4381 tcgcattatc tggtccctgc ggtaccaccg cgatacaacg gtaaaccgcg catctggcaa
     4441 ctggtgccgg aacaagaact ggcctggcat gcggggatta gcgcctggcg cggggcaacg
     4501 cgccttaacg acacctctat tggcattgag ctggaaaacc gtggctggca aaaatcggcc
     4561 ggagtgaaat attttgcccc gtttgaaccg gcacagattc aggcgcttat tccactggcg
     4621 aaagatatta ttgcccgtta tcacatcaag ccggaaaacg tagtggcaca tgcggatatc
     4681 gcaccgcagc gcaaagacga tccggggcca ttatttccct ggcagcaact ggcgcagcag
     4741 gggattggtg cctggccgga tgcgcagcgg gttaactttt accttgccgg gcgcgcgccg
     4801 cacacaccgg tagataccgc gtcattgctg gagcttttgg cgcgctacgg ttatgacgtt
     4861 aaacctgata tgacaccgcg cgagcagcgg cgcgtgatta tggcattcca gatgcatttc
     4921 cgcccgacgt tatataacgg cgaagcggat gctgaaactc aggcgattgc cgaagcattg
     4981 ctggagaaat acgggcagga ttagcgcggc agttttccgt ggtcgcgtag ccaggcggca
     5041 gttttctcga taccttcatc cagggtgatg accggctgat aacctaactc ttcctgcgca
     5101 cgcgtaatat ccagcgtaaa gtcaaaattc aacttggaga cgccgtagtg ggtcagcggc
     5161 ggctcttttg ctgacttgcg gcctaaacgc tccatgctgc gggcgatcat atccagcatc
     5221 gggtagggga cggaacgaat acgacagtca atattcaact cgtcgatcag cttctgcacg
     5281 atgctgcgca gtgtgcgatg ctcgccgttg gtgatgttgt acacacgccc ggaaggtagc
     5341 ttatcgcagg cttcctggct tgccagccac attgcgtgca cggcattttc atagtaggtc
     5401 atatcgacca gcgcactgcc gccatgcggt aacagaatac tgccgtagtg gtgcatcata
     5461 tgcgccagac ggggaataaa gactttatcg tgcggtccga acagactttg tgggcgcaga
     5521 atagtaaagc gcgtttgtgg attcgcctgc gaaagcatat tgatcacttc ttcgctggct
     5581 gctttgctgc gggcaaactc gttggcgaag cggtgagggc gaaaatcttc tttaatatcg
     5641 cgatggtggt gataatcgaa gtacagggag ggggaagaga tatgaataaa gttacgcaca
     5701 ccccaggcga cagtccattc acccaggcgg cgagtggcgc gaacgttagc cagatcgaaa
     5761 gcctgttgtg tcccccaggg tgaggtaaag ctggagcagt gccacagcgt atcaatgccc
     5821 gcgagcatca ctttagcttg tgatgaaacc agctcggtca gatccgccgg aacaaactct
     5881 gcgcccattt tttccagcaa tttgcccatt gcctcgttgc gaccggtcgc tcgcacgctg
     5941 atgcctttct ggcataaaaa ctctaccgcg tttcgaccta agccgctggt ggcgccggta
     6001 accagtacct tcatatcaat ccactgttgt tgagaaaata acgtgcgcat tcttccgtga
     6061 tttcccccat gatgcaatgg gaaacatgaa agaataacgc aggttttgtc gattaatctg
     6121 tgctttgttc tgccagtctg gcgatttgtt ttgccattcc gcgaaaaata aacagatgcg
     6181 cggggatcat caataaccag taaaacagcc ccggcatacc gtgcggatgc cagaaagcgc
     6241 ggacatcgat agtactatag tcgcctttat cttctaggct aaaacacagt cgtcccagcc
     6301 ccggcgcttt catgccaaat aacaacgtaa gttgtttttc cggttcaacg acaatcactt
     6361 tccagctatc caccgcatcg ccagtctgta aatattcgcg ctccgggcgg cctttcgcca
     6421 gcttatgacc gatcgcgcgg tccatcaacg cccgtgtctg ccacaaaata ttgccaaaga
     6481 aataacgctc tttaccgccg atttggttca ctacctgcca taaagcagca aggctggcgg
     6541 acgttttaac ggtaaacccc gcctgtttgg caaaataacc gtactctggt cgccagcggg
     6601 caaaggcctg agcgtcgtag ccccagtcgc tggagttgac cagtttttcc tcctctttca
     6661 acgtgctacg taccgcgtca tcgaaagcga tcagccgttg tgggatgagt gcacgtagcg
     6721 cggtatcatc cgccagcaga tcgtgtttca gcccctgaat caacgccctg gcggtggtgg
     6781 gcggtacgga agtaatcaca ttgaga
//
LOCUS       DAZKHF020000232         6792 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2201S31241
            SAMN43267800-rid25106413.denovo.234, whole genome shotgun sequence.
ACCESSION   DAZKHF020000232 DAZKHF020000000
VERSION     DAZKHF020000232.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267800
            Sequence Read Archive: SRR30307263
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6792)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6792)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 6792)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350581.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350581.2
            Genome Coverage       :: 151x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:21:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,652
            CDSs (total)                      :: 5,557
            Genes (coding)                    :: 5,320
            CDSs (with protein)               :: 5,320
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 237
            CDSs (without protein)            :: 237
            Pseudo Genes (ambiguous residues) :: 0 of 237
            Pseudo Genes (frameshifted)       :: 102 of 237
            Pseudo Genes (incomplete)         :: 130 of 237
            Pseudo Genes (internal stop)      :: 52 of 237
            Pseudo Genes (multiple problems)  :: 41 of 237
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6792
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267800-rid25106413.denovo.234"
                     /serotype="O 26:H11"
                     /isolate="2201S31241"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-01"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            <1..2868
                     /gene="evgS"
                     /locus_tag="ACHOSC_RS19340"
                     /old_locus_tag="pgaptmp_003868"
     CDS             <1..2868
                     /gene="evgS"
                     /locus_tag="ACHOSC_RS19340"
                     /old_locus_tag="pgaptmp_003868"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_process: GO:0010447 - response to acidic pH [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acid-sensing system histidine kinase EvgS"
                     /protein_id="HGU4549713.1"
                     /translation="FLTRKESVILNEVLNRFVDALTNEVRYEVSQNWLDTGNLAFLNK
                     PLELTEHEKQWIKQHPDLKVLENPYSPPYSMTDENGSVRGVMGDILNIITLQTGLNFS
                     PITVSHNIHAGTQLNPGGWDIIPGAIYSEDRENNVSFAEAFITTPYVFVMQKAPDSEQ
                     TLKKGMKVAIPYYYELHSQLKEMYPEVEWIQVDNASAAFHKVKEGELDALVATQLNSR
                     YMIDHYYPNELYHFLIPGVPNASLSFAFPRGEPELKDIINKALNAIPPSEVLRLTEKW
                     IKMPNVTIDTWDLYSEQFYIVTTLSVLLVGSSLLWGFYLLRSVRRRKVIQGDLENQIS
                     FRKALSDSLPNPTYVVNWQGNVISHNSAFEHYFTADYYKNAMLPLENSDSPFKDVFSN
                     AHEVTAETKENRTIYTQVFEIDNGIEKRCINHWHTLCNLPASDNAVYICGWQDITETR
                     DLINALEVEKNKAIKATVAKSQFLATMSHEIRTPISSIMGFLELLSGSGLSKEQRVEA
                     ISLAYATGQSLLGLIGEILDVDKIESGNYQLQPQWVDIPTLVQNTCHSFGAIAASKSI
                     ALSCSSTFPDHYLVKIDPQAFKQVLSNLLSNALKFTTEGAVKITTSLVHIDDNHAVIK
                     MTIMDSGSGLSQEEQQQLFKRYSQTSAGRQQTGSGLGLMICKELIKNMQGDLSLESHP
                     GIGTTFTITIPVEISQQVATVEAKAEHPITLPEKLSILIADDHPTNRLLLKRQLNLLG
                     YDVDEATDGVQALHKVSMQHYDLLITDVNMPNMDGFELTRKLREQNSSLPIWGLTANA
                     QANEREKGLSCGMNLCLFKPLTLDVLKTHLSQLHQVAHIAPQYRHLDIEALKNNTAND
                     LQLMQEILMTFQHETHKDLPAAFQALEAGDNRTFHQCIHRIHGAANILNLQKLINISH
                     QLEITPVSDDSKPEILQLLNSVKEHIAELDQEIAVFCQKND"
     gene            complement(2924..4069)
                     /gene="yfdE"
                     /locus_tag="ACHOSC_RS19345"
                     /old_locus_tag="pgaptmp_003869"
     CDS             complement(2924..4069)
                     /gene="yfdE"
                     /locus_tag="ACHOSC_RS19345"
                     /old_locus_tag="pgaptmp_003869"
                     /EC_number="2.8.3.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416872.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA:oxalate CoA-transferase"
                     /protein_id="HGU4549714.1"
                     /translation="MTNNESKGPFEGLLVIDMTHVLNGPFGTQLLCNMGARVIKVEPP
                     GHGDDTRTFGPYVDGQSLYYSFINHGKESVVLDLKNDHDKSIFINMLKQADVLAENFR
                     PGTMEKLGFSWETLQEINPRLIYASSSGFGHTGPLKDAPAYDTIIQAMSGIMMETGYP
                     DAPPVRVGTSLADLCGGVYLFSGIVSALYGREKSQRGAHVDIAMFDATLSFLEHGLMA
                     YIATGKSPQRLGNRHPYMAPFDVFNTQDKPITICCGNDKLFSALCQALELTELVNDPR
                     FSSNILRVQNQAILKQYIERTLKTQAAEVWLARIHEVGVPVAPLLSVAEAIKLPQTQA
                     RNMLIEAGGIMMPGNPIKISGCADPHVMPGAATLDQHGEQIRQEFSS"
     gene            complement(4143..5087)
                     /gene="yfdV"
                     /locus_tag="ACHOSC_RS19350"
                     /old_locus_tag="pgaptmp_003870"
     CDS             complement(4143..5087)
                     /gene="yfdV"
                     /locus_tag="ACHOSC_RS19350"
                     /old_locus_tag="pgaptmp_003870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter YfdV"
                     /protein_id="HGU4549715.1"
                     /translation="MLTFFIGDLLPIIVIMLLGYFSGRRETFSEDQARAFNKLVLNYA
                     LPAALFVSITRANREMIFADTRLTLVSLVVIVGCFFFSWFGCYKFFKRTHAEAAVCAL
                     IAGSPTIGFLGFAVLDPIYGDSVSTGLVVAIISIIVNAITIPIGLYLLNPSSGADGKK
                     NSNLSALISAAKEPVVWAPVLATILVLVGVKIPAAWDPTFNLIAKANSGVAVFAAGLT
                     LAAHKFEFSAEIAYNTFLKLILMPLALLLVGMACHLNSEHLQMMVLAGALPPAFSGII
                     IASRFNVYTRTGTASLAVSVLGFVVTAPLWIYVSRLVS"
     gene            complement(5157..>6792)
                     /gene="oxc"
                     /locus_tag="ACHOSC_RS19355"
                     /old_locus_tag="pgaptmp_003871"
     CDS             complement(5157..>6792)
                     /gene="oxc"
                     /locus_tag="ACHOSC_RS19355"
                     /old_locus_tag="pgaptmp_003871"
                     /EC_number="4.1.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008949 - oxalyl-CoA decarboxylase
                     activity [Evidence IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_process: GO:0033611 - oxalate catabolic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="oxalyl-CoA decarboxylase"
                     /protein_id="HGU4549716.1"
                     /translation="NNIDTIYGVVGIPVTDMARHAQAEGIRYIGFRHEQSAGYAAAAS
                     GFLTQKPGICLTVSAPGFLNGLTALANATVNGFPMIMISGSSDRAIVDLQQGDYEELD
                     QMNAAKPYAKAAFRVNQPQDLGIALARAIRVSVSGRPGGVYLDLPANVLAATMEKDEA
                     LTTIVKVENPSPALLPCPKSVTSAISLLAKAERPLIILGKGAAYSQADEQLREFIEST
                     QIPFLPMSMAKGILEDTHPLSAAAARSFALANADVVMLVGARLNWLLAHGKKGWAADT
                     QFIQLDIEPQEIDSNRPIAVPVVGDIASSMQGMLAELKQNTFTTPLVWRDILNIHKQQ
                     NAQKMHEKLSTDTQPLNYFNALSAVRDVLRENQDIYLVNEGANTLDNARNIIDMYKPR
                     RRLDCGTWGVMGIGMGYAIGASVTSGSPVVAIEGDSAFGFSGMEIETICRYNLPVTIV
                     IFNNGGIYRGDGVDLSGAGAPSPTDLLHHARYDKLMDAFRGVGYNVTTTDELRHALTT
                     GIQSRKPTIINVVIDPAAGTESGHITKLNPKQVAGN"
BASE COUNT         2021 a         1561 c         1412 g         1798 t
ORIGIN      
        1 ttcttgacca gaaaagaatc cgtcattctt aatgaagtac tcaatagatt tgttgatgct
       61 ttaacaaatg aagttcgcta tgaagtttca caaaattggc ttgatacagg aaacctggcc
      121 tttctgaaca aaccattaga actcactgaa catgaaaaac agtggattaa gcaacatccc
      181 gatttaaagg tgctggaaaa tccttactct cctccctatt ctatgacgga tgaaaatggc
      241 tcggttcggg gcgttatggg ggatattctt aatattatta ccttgcaaac aggtttaaat
      301 ttttctccga tcaccgtttc acacaatatc catgccggaa cacagcttaa ccccggtgga
      361 tgggatataa tacctggcgc tatttatagt gaagatcgag aaaataatgt ttcatttgca
      421 gaagctttca taacaacgcc atacgttttt gtcatgcaaa aagcacctga cagtgaacaa
      481 acattaaaaa aaggaatgaa agttgccatt ccatattatt atgagctgca ttcgcaatta
      541 aaagagatgt atccggaggt tgaatggata caggtcgata atgccagcgc tgcatttcac
      601 aaggttaagg aaggtgaact tgatgctctg gtcgcgacac agttaaattc gcgttacatg
      661 atcgatcatt actatcctaa tgaactttat cattttctta ttcctggcgt tccgaatgca
      721 tcgctttcgt tcgcttttcc tcgcggagaa ccggaactta aggatattat taataaagca
      781 ctgaatgcaa ttcccccaag cgaagttctg cgcctgacgg aaaaatggat taaaatgccc
      841 aatgtgacca ttgacacatg ggacctatat agcgagcaat tttatattgt tacgacatta
      901 tccgttttat tagttggcag tagcctttta tggggattct acctgttacg ctcagttcgt
      961 cgtcgtaaag tcattcaggg tgatttagaa aaccaaatat cattccgaaa agcactctcg
     1021 gattccttac cgaatccaac ttatgttgta aactggcaag gtaatgtcat tagtcataat
     1081 agtgcttttg aacattattt cactgcggat tactacaaaa atgcaatgtt accattagaa
     1141 aacagtgact caccctttaa agatgttttt tctaatgcgc atgaagtcac agcagaaacg
     1201 aaagaaaatc gaacaatata cacacaggta tttgaaattg ataatggcat cgagaaaaga
     1261 tgcattaatc actggcatac attatgcaat cttcctgcaa gtgacaatgc agtatatatt
     1321 tgtggttggc aagatattac tgaaacgcgt gatctaatta atgcactcga ggtagaaaaa
     1381 aataaagcga taaaggctac cgtagcaaaa agtcagtttc tggcaacgat gagtcacgaa
     1441 ataagaacac caataagctc tattatgggc ttcctggaac ttctgtcggg ttctggtctt
     1501 agcaaggagc aacgggtgga ggcgatttca cttgcctacg ccaccggaca atcactcctc
     1561 ggcttaattg gtgaaatcct tgatgtcgac aaaattgaat cgggtaacta tcaacttcaa
     1621 ccacaatggg tcgatatccc tactttagtc cagaacactt gtcactcttt cggtgcgatt
     1681 gctgcaagca aatcgatcgc attaagttgc agcagtacat ttcctgatca ttacctggtt
     1741 aagatcgacc ctcaggcgtt taagcaggtc ttatcaaatt tactgagcaa tgctctcaaa
     1801 tttaccaccg agggggcagt aaaaattacg acctccctgg ttcacattga tgacaaccac
     1861 gctgttatca aaatgacgat tatggattct ggaagtggat tatcgcagga agaacaacaa
     1921 caactcttta aacgctacag ccaaacaagt gcaggtcgcc agcaaacagg atctggctta
     1981 ggcttaatga tctgcaaaga attaattaaa aatatgcagg gcgatttgtc attagaaagt
     2041 catccaggca taggaacaac atttacgatc acaatcccgg tagaaattag ccagcaagtg
     2101 gcgactgtcg aggcaaaagc agaacacccc atcacactac ctgaaaagtt gagcatatta
     2161 atcgcggatg atcatccgac caacaggcta ttactcaaac gccagctaaa tctattagga
     2221 tatgatgttg atgaagccac tgatggtgtg caagcgctac acaaagtcag tatgcaacat
     2281 tatgatctgc ttattactga cgttaatatg ccgaatatgg atggttttga gttgactcgc
     2341 aaactccgtg agcaaaattc ttccttaccc atctgggggc ttacagccaa cgcacaggct
     2401 aacgaacgtg aaaaagggtt aagttgcggc atgaacttat gtttgttcaa accgttgacc
     2461 ctggatgtac tgaaaacaca tttaagtcag ttacaccagg ttgcgcatat tgcacctcag
     2521 tatcgccacc ttgatatcga agccctgaaa aataatacgg cgaacgatct acaactgatg
     2581 caggagattc tcatgacttt ccagcatgaa acgcataaag atctacccgc tgcgtttcaa
     2641 gcactagaag ctggcgataa cagaactttc catcagtgta ttcatcgcat ccacggtgcg
     2701 gctaacatcc tgaatttgca aaagttgatt aatattagcc atcagttaga aataacacct
     2761 gtttcagatg acagtaagcc tgaaattctt cagttgctga actctgtaaa agaacacatt
     2821 gcagagctgg accaggagat tgctgttttc tgtcagaaaa atgactaaat agcggctccc
     2881 acaatgttca aatgtgggag ctatttacca gcacatctta catttatgat gagaactcct
     2941 ggcgaatttg ttccccatgc tggtcgagcg ttgccgctcc cggcataaca tgcgggtccg
     3001 cgcagccgct gatttttatc ggattacccg gcatcattat tcccccggct tcaatcaaca
     3061 tatttctcgc ctgagtttgt ggcaatttaa tggcctcagc cacacttaat aacggcgcga
     3121 cgggtacacc aacttcatgt attctggcta accaaacttc agctgcctgc gtttttaacg
     3181 tccgctcaat atattgttta agaatagcct ggttttgtac gcgtaaaata ttgctgctaa
     3241 atcggggatc attaaccagt tccgtaagct ccagtgcctg gcataacgca gaaaaaagct
     3301 tgtcattacc acaacaaatc gtaatcggct tatcctgagt attgaaaaca tcaaaaggtg
     3361 ccatgtaggg atggcgattt cccagacgtt gtggtgactt ccctgtcgcg atatatgcca
     3421 tcagaccatg ctccagaaaa ctcagcgtgg catcaaacat cgctatatcg acatgcgccc
     3481 ctctctggct cttttcgcgg ccataaagtg cactcactat tccgctgaat aaatagacac
     3541 cgccgcatag atccgcaaga gatgtaccaa cgcgcactgg cggagcatca ggatatcctg
     3601 tttccatcat tatcccgctc attgcctgaa tgatggtatc gtaggcagga gcatctttta
     3661 gcggaccggt atgtccgaaa cctgacgatg aagcatatat gaggcgcggg ttgatttctt
     3721 gaagcgtttc ccatgaaaac cccagttttt ccattgtacc tgggcgaaaa ttctcagcta
     3781 atacatcagc ttgtttgagc atatttataa atatactttt atcgtgatca ttctttaaat
     3841 caagaaccac actctctttg ccatgattaa taaaactgta atagagtgac tgtccatcca
     3901 catagggacc aaatgtgcgg gtatcatcac catgacccgg cggctcaact ttaattaccc
     3961 ttgcgcccat attacaaaga agttgagttc cgaaaggtcc attaaggaca tgtgtcatat
     4021 cgataactaa taagccttca aacggccctt tgctttcatt atttgtcatt tgcttatgcc
     4081 ttgaaaaaag ccgaaggtga aaagacactt cggcatttat gtaaaatcaa taacatgatt
     4141 aattatgaaa ccagtcgact gacataaatc cacaagggag ccgtgacgac aaagcccagt
     4201 acgctcacag ccaatgacgc tgtaccagta cgggtgtaga cattaaaccg gctggcaata
     4261 atgattccgg agaatgccgg cggtaatgcg cctgccagta ccatcatctg cagatgttcg
     4321 ctgttcaaat gacatgccat accaacgaga agcagtgcca gtggcatcag aatcagcttc
     4381 aggaaggtgt tataagcaat ttcggcactg aactcgaatt tatgtgcagc cagagtcaac
     4441 cccgcagcga ataccgctac ccctgagtta gccttcgcaa tcagattaaa ggttgggtcc
     4501 catgctgccg gaatttttac cccaaccaac accaggatcg ttgccagaac aggtgcccat
     4561 actactggct cctttgccgc agaaattaat gcgctcagat tactattctt cttaccatcc
     4621 gctcctgagg aaggattcag caaatacaga ccaataggaa tagtaattgc gttaacaata
     4681 atagaaataa ttgctaccac taaacctgtt gatacggaat caccataaat aggatcgaga
     4741 actgcaaacc ccaggaatcc aatggtaggt gaacccgcaa ttaatgcaca tacagctgct
     4801 tctgcatggg tacgtttaaa aaatttgtag caaccaaacc aggagaaaaa gaaacatccg
     4861 acaataacca caagtgatac aagggtcaga cgagtgtccg caaaaatcat ttccctgttt
     4921 gcccgggtaa tagaaacaaa tagagccgca ggaagagcgt agtttaatac cagtttatta
     4981 aatgcgcggg cttgatcttc tgaaaaagtt tctcgtctgc cgctaaaata acccaacagc
     5041 atgattacga taataggcaa taaatcgcca ataaaaaatg ttagcataat agtatttccg
     5101 ataagggtcc cctcaccatc gcataatgag ttggcaagag gagacgatgt cagtatttaa
     5161 ttaccagcga cttgttttgg gttaagtttg gtaatatggc cactttcagt tcctgctgca
     5221 gggtcgatga ccacattaat aatggtcggt ttgcgcgact gaataccggt ggttaaagca
     5281 tgacgaagtt catctgtcgt ggtgacgtta tagccaacgc cacgaaacgc atccattaat
     5341 ttgtcatacc ttgcatggtg caacagatcc gttggtgatg gtgcaccagc gccactgaga
     5401 tcaacaccgt ctcctctgta gatgccgcca ttattaaaaa taacgatcgt caccggcagg
     5461 ttatatcgac aaatcgtttc aatttccatc ccactgaaac caaaagcact atcaccttca
     5521 atggcgacaa ccggagaacc agaagtcacg ctagcaccga tggcatagcc cataccgatg
     5581 cccatgacac cccaggtacc acaatccaga cgacgacgtg gtttatacat atcaataata
     5641 tttcgtgcat tatccagggt atttgcacct tcattaacta aataaatatc ctggttctcg
     5701 cgcaatacgt cgcgcacagc acttaatgca ttaaagtaat ttaatggttg agtatctgta
     5761 cttaattttt catgcatttt ttgtgcattt tgctgcttgt ggatatttaa aatatcgcgc
     5821 cataccagtg gagtcgtaaa tgtgttttgt ttcagttctg ccagcatacc ttgcatactg
     5881 gatgcaatat caccaacgac tggcacagca atggggcggt tgctgtcaat ttcctgcggt
     5941 tcaatatcca gttgaataaa ctgtgtatct gccgcccatc cttttttacc gtgtgccaat
     6001 aaccaattca gtcgtgcacc aacaagcatg acaacgtcag catttgccag ggcaaacgaa
     6061 cgcgcagctg ccgcagaaag tggatgcgta tcttcaagga tccctttcgc catagacatt
     6121 ggcaggaatg gaatctgagt actttcaata aactcacgaa gctgttcatc agcttgtgaa
     6181 tacgccgcgc ctttgccaag gataattaat ggccgttcag cttttgctaa aagcgaaatt
     6241 gcgctagtga ctgacttcgg gcatggcaat aatgctggcg acggattttc aactttaaca
     6301 atcgtggtta acgcttcgtc tttttccatc gtcgcggcca ggacatttgc tggcaaatca
     6361 agataaactc cgccagggcg acccgataca gagacccgga tagcgcgtgc caatgcaatg
     6421 ccaagatcct gcggctgatt aacgcgaaat gctgctttgg catacggttt tgccgcattc
     6481 atttggtcca gctcttcata atcaccttgc tgtaggtcga cgatcgcgcg gtcgctggag
     6541 ccgctaatca tgatcatcgg aaaaccattt accgttgcgt tggccaatgc ggtcaaacca
     6601 ttgaggaatc ctggcgcaga aactgtcagg cagatccccg gtttttgggt aagaaaaccg
     6661 cttgccgcag cggcatagcc tgccgactgc tcatgacgaa aaccaatata acgaatgcct
     6721 tccgcctggg catggcgtgc catatccgtc acaggaatac ctacaacacc ataaatagtg
     6781 tcaatattat tc
//
LOCUS       DAZKHF020000233         6779 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2201S31241
            SAMN43267800-rid25106413.denovo.235, whole genome shotgun sequence.
ACCESSION   DAZKHF020000233 DAZKHF020000000
VERSION     DAZKHF020000233.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267800
            Sequence Read Archive: SRR30307263
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6779)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6779)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 6779)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350581.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350581.2
            Genome Coverage       :: 151x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:21:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,652
            CDSs (total)                      :: 5,557
            Genes (coding)                    :: 5,320
            CDSs (with protein)               :: 5,320
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 237
            CDSs (without protein)            :: 237
            Pseudo Genes (ambiguous residues) :: 0 of 237
            Pseudo Genes (frameshifted)       :: 102 of 237
            Pseudo Genes (incomplete)         :: 130 of 237
            Pseudo Genes (internal stop)      :: 52 of 237
            Pseudo Genes (multiple problems)  :: 41 of 237
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6779
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267800-rid25106413.denovo.235"
                     /serotype="O 26:H11"
                     /isolate="2201S31241"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-01"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            complement(10..543)
                     /locus_tag="ACHOSC_RS19360"
                     /old_locus_tag="pgaptmp_003872"
                     /pseudo
     CDS             complement(10..543)
                     /locus_tag="ACHOSC_RS19360"
                     /old_locus_tag="pgaptmp_003872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000498860.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
     gene            complement(548..712)
                     /locus_tag="ACHOSC_RS19365"
                     /old_locus_tag="pgaptmp_003873"
     CDS             complement(548..712)
                     /locus_tag="ACHOSC_RS19365"
                     /old_locus_tag="pgaptmp_003873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4549717.1"
                     /translation="MSGWCRPEIMILTMKVKCIGSLIGRSEAAVRMKAQVKGISLILR
                     GDFHQSTKYP"
     gene            complement(709..1442)
                     /locus_tag="ACHOSC_RS19370"
                     /old_locus_tag="pgaptmp_003874"
                     /pseudo
     CDS             complement(709..1442)
                     /locus_tag="ACHOSC_RS19370"
                     /old_locus_tag="pgaptmp_003874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001585548.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1627 domain-containing protein"
     gene            complement(1476..1937)
                     /locus_tag="ACHOSC_RS19375"
                     /old_locus_tag="pgaptmp_003875"
     CDS             complement(1476..1937)
                     /locus_tag="ACHOSC_RS19375"
                     /old_locus_tag="pgaptmp_003875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310232.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replication protein P"
                     /protein_id="HGU4549718.1"
                     /translation="MTEQQIRQVCRQCMDRCRAGETWPPDLAEFVALISKSGANPFGL
                     TVDAVMEEYRRWRNESWRYDGSDKYPWSQPVLYHICLEMRSKGIERQMTEGELKRLAE
                     RQLTKWAKHVSNGLSVPPVRRQLAAPKRPSGPTPIELLKQEYERRKAAGFV"
     gene            complement(1930..2970)
                     /locus_tag="ACHOSC_RS19380"
                     /old_locus_tag="pgaptmp_003876"
     CDS             complement(1930..2970)
                     /locus_tag="ACHOSC_RS19380"
                     /old_locus_tag="pgaptmp_003876"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaT-like ssDNA-binding domain-containing
                     protein"
                     /protein_id="HGU4549719.1"
                     /translation="MSIDALRWAKKVKTGSSSSKSVLTWLADMCGADLCAYPSVSALA
                     EVTELNKKTVQDSLRHLMEIGLIVDTGERKGRTKQIVVYRLIGVEESVAEPEYTQKRE
                     SLKVGKIGAVNKNSTENGYVSAQNRPKNRTLSCMENNQRHPNFPSKTPKNGSRNPKEP
                     KDLNPTHNARESAPTSEQEVLSLQAAPPVFLYGLSEPIGKFPMSDSWYPSRDFRRRAA
                     LWGMALPETEFTPAELAAFRDYWAAEGKVFTQIQWEQKFARHVNHVRAQVKPVSKGVN
                     HAAAPGGTASRAVQEIRAAREQWERENGFISDGNGLEAVGTHGGGLFEPLDPEERGRT
                     FEALDCTDWRDD"
     gene            complement(2942..3493)
                     /locus_tag="ACHOSC_RS19385"
                     /old_locus_tag="pgaptmp_003877"
     CDS             complement(2942..3493)
                     /locus_tag="ACHOSC_RS19385"
                     /old_locus_tag="pgaptmp_003877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxin YdaT family protein"
                     /protein_id="HGU4549720.1"
                     /translation="MKITTEQVCEALDTWVCRPGMTQEQATILITEAFWALKERPNID
                     VQRVTFNDGEVDQRALGVNRVKIFERWKAIDTRDKRDKFTALIPAIMEAIRISDFRLY
                     CEITDGKSITYMIAGLNKEYGDVVESGLLFADPVVVERETDELIEKAIAFKHAYRQQY
                     QYYFADKQMSARGSYEYRCTTMG"
     gene            complement(3477..3704)
                     /locus_tag="ACHOSC_RS19390"
                     /old_locus_tag="pgaptmp_003878"
     CDS             complement(3477..3704)
                     /locus_tag="ACHOSC_RS19390"
                     /old_locus_tag="pgaptmp_003878"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cro/CI family transcriptional regulator"
                     /protein_id="HGU4549721.1"
                     /translation="MLKIDAIAFFGSKTKLANVAGVRLASVAAWGELVPEGRAMRLQE
                     ASGGELQYDPKVYDEYRKAKRAGRLNNENHH"
     gene            3781..4188
                     /locus_tag="ACHOSC_RS19395"
                     /old_locus_tag="pgaptmp_003879"
     CDS             3781..4188
                     /locus_tag="ACHOSC_RS19395"
                     /old_locus_tag="pgaptmp_003879"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HGU4549722.1"
                     /translation="MKNETFGARLLYRRKKLKLSQAALGKLVKVAHVTISQWERDETQ
                     PAGKRLFALSQALQCSPTWLLFGDEDKQPGEPIPDNQPVNLTEDQKELLQLFDALPES
                     EQKALLSEMRARVENFNKLFEELLKARKRSANK"
     gene            4453..4752
                     /locus_tag="ACHOSC_RS19400"
                     /old_locus_tag="pgaptmp_003880"
     CDS             4453..4752
                     /locus_tag="ACHOSC_RS19400"
                     /old_locus_tag="pgaptmp_003880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4549723.1"
                     /translation="MALYIDISAIAGQVRVIRAVTKRYAPLLQKVSGECTEDIVNDFV
                     IELRGLIFSYKVTTIFADGSRETVRALRLKGCVKDLATTFWARKLDCIHNQFPLE"
     gene            4825..5043
                     /gene="ydfC"
                     /locus_tag="ACHOSC_RS19405"
                     /old_locus_tag="pgaptmp_003881"
     CDS             4825..5043
                     /gene="ydfC"
                     /locus_tag="ACHOSC_RS19405"
                     /old_locus_tag="pgaptmp_003881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YdfC"
                     /protein_id="HGU4549724.1"
                     /translation="MQKRDPVIIAPDYTDDELYEWMHQKIKAVQDLKWANEARAKQAE
                     NLSALEQDITNLEKAAALSIARMITYPR"
     gene            5066..5473
                     /locus_tag="ACHOSC_RS19410"
                     /old_locus_tag="pgaptmp_003882"
     CDS             5066..5473
                     /locus_tag="ACHOSC_RS19410"
                     /old_locus_tag="pgaptmp_003882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4549725.1"
                     /translation="MEFKDLPTPLQEMASNIVRSQLATLDLSTAEKETIDNMVRNVRN
                     AFSGLYGSDNQKQESDVNKRVISVCVNGHVLSSIKTETATVFDCLCIVQSLVDALFRS
                     VNLENNANLRGRIIAHPYAHTLGSVDIKDPTNL"
     gene            complement(5451..5684)
                     /locus_tag="ACHOSC_RS19415"
                     /old_locus_tag="pgaptmp_003883"
     CDS             complement(5451..5684)
                     /locus_tag="ACHOSC_RS19415"
                     /old_locus_tag="pgaptmp_003883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4549726.1"
                     /translation="MLKSTLIAKCLYQNRMVSSISIGESAVKSIFEEYFPGHDFNKWN
                     TKLPPAVSTRILKATERASTIRVNYFIKDLWDL"
     gene            6245..6433
                     /gene="dicB"
                     /locus_tag="ACHOSC_RS19420"
                     /old_locus_tag="pgaptmp_003884"
     CDS             6245..6433
                     /gene="dicB"
                     /locus_tag="ACHOSC_RS19420"
                     /old_locus_tag="pgaptmp_003884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division inhibition protein DicB"
                     /protein_id="HGU4549727.1"
                     /translation="METLLPNVNTSEGCFDIGVLLSNREFTEDAIKMRKYEPYLLNDN
                     SILSRIALLELGIIGEQQ"
     gene            6430..6618
                     /locus_tag="ACHOSC_RS19425"
                     /old_locus_tag="pgaptmp_003885"
     CDS             6430..6618
                     /locus_tag="ACHOSC_RS19425"
                     /old_locus_tag="pgaptmp_003885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1482 family protein"
                     /protein_id="HGU4549728.1"
                     /translation="MTSAFALVMTVFLITGEPQNVITGIYDSKSSCIQVRDEQKIPGE
                     CLPLKKVSLNLNNEIPAG"
     gene            6714..>6779
                     /locus_tag="ACHOSC_RS19430"
                     /old_locus_tag="pgaptmp_003886"
     CDS             6714..>6779
                     /locus_tag="ACHOSC_RS19430"
                     /old_locus_tag="pgaptmp_003886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001757322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease"
                     /protein_id="HGU4549729.1"
                     /translation="MSKVFICAAIPDEQAIKEEGAV"
BASE COUNT         1717 a         1727 c         1501 g         1834 t
ORIGIN      
        1 gtataaacct tacatgttga ttacattttt gtaatcaaca tcctgtttgg aatagccagc
       61 ctttaatgga taactatttc tgacaatgca atgagtataa tcaagtccat cttccactga
      121 gaattagagg cggcatgctt tttccggctc ttgccggata tccgtaattg tccataatct
      181 gcagatttat accttctgca tgacctgtca gcgaaaattt gtccggtgtt tctacgggaa
      241 tgacatcaaa agttacacgc actcgcgtta ccgtgtagac ctactttcct gcacttgcaa
      301 gatcacagtg gtgtaaccgt aacaggaatt tattctctgg accggcagta aatccctgag
      361 tggcgtggtt cccatatcaa tttcccgcca ggcagcctcc attgccagcg tacaggctgg
      421 agccatgacc tgccctttaa atctggcccg accatcccac cggacgtgtt cttctcccct
      481 gaacttaggt acagtcatct ccagtggcac aaaagtgtca gcgccataat ttttgaccgt
      541 tatcgcgcta cggatatttt gttgactggt gaaaatcacc ccgcagaatc aggcttattc
      601 ccttaacctg ggctttcatc ctgaccgccg cctcactacg accaatcaga ctgccgatgc
      661 attttacctt cattgttagt atcatgattt caggcctgca ccatccgctc attgcccgga
      721 cttccgacaa atcccggcaa ccatatcccg gtgcttgttc agctcccgca gcgcggcgca
      781 gactcgctcc cacttctgga catgattctt cgccctacgc agttcgcgat ttgccatatg
      841 cagcgatggc aaaatcaaat catcttctcg cgttgcagta aacgatggca gcgactgcac
      901 aatgtccgcc acagtttctg ttttaatatc ttcctgtgtt gcatctttct gtaccggtaa
      961 cgaacaccgg ctggctgagg aaaggcttta ccatcggttt ccgctaccga ttcaactttc
     1021 ggctctgctg gtaaattatc acctggtatg cagtaacgaa ttttaccgtt ctggtttacg
     1081 cgaatcagac gacctttgct gattgccatt gccagcgttg aagcaacttt gcgggatgtt
     1141 gtaccgaaca gcgtagccag ttcatccgcc gtttgtggtc cgcgttgttc aatcgtcgcg
     1201 gttaaatcgc actctgagat tttcgcttct gttgccgtgg tggtttcttc tggcgctggc
     1261 tgttcctgct gaacgttgtt atcagccaca cgccaggtgt atacgctttt atcaacgaag
     1321 ccagcctttt tcagttccca cagctcgttc agtacttctt cacgactgat atcaagtcgc
     1381 gcagccagct ctaccgacgt ggcttttccc atcgctttca gtgcgtcaaa aacagtctcc
     1441 ataaatttcc tcccggtaaa aaatcacttc tcaactcaaa caaaaccagc cgctttccgg
     1501 cgttcatatt cctgtttcag caactcaatt ggcgttggcc ccgacgggcg tttgggggcc
     1561 gccagttgtc gccggactgg cggaacgctc aggccgttac taacatgctt tgcccatttc
     1621 gtcagttgcc gttctgcaag ccgttttaat tccccttcgg tcatctggcg ctcaatcccc
     1681 tttgaacgca tctcgaggca aatgtgatac agcacaggct gagaccacgg gtacttatca
     1741 cttccgtcgt atcgccagga ctcattgcgc cagcggcggt actcctccat cacagcatcc
     1801 accgtcagac cgaatggatt ggctccgctt ttcgaaatca gtgccacaaa ctcagccagg
     1861 tccggaggcc atgtttcacc cgcccggcag cggtccatgc actggcggca gacctgccgg
     1921 atttgctgct cagtcatcgc gccaatctgt gcaatccaga gcttcgaagg tgcggccccg
     1981 ttcttctgga tccagcggtt cgaataaacc acccccatga gttcccacag cttccaggcc
     2041 gtttccgtcg ctgataaatc cgttttcacg ttcccactgc tcacgtgctg cccgaatttc
     2101 ctgaactgcc cgtgatgcgg tgccacctgg tgctgctgca tggtttaccc ctttgctgac
     2161 tggtttaacc tgcgccctga cgtgatttac gtgacgggcg aatttctgct cccactgaat
     2221 ctgcgtaaac actttcccct ccgctgccca gtagtcccgg aaggcggcaa gttcagcagg
     2281 tgtaaattct gtctccggca aagccatccc ccacaacgca gcccgtcgtc gaaaatcccg
     2341 tgacggatac cagctatcgc tcatcgggaa ttttccgatg ggttcgctca ggccatacag
     2401 gaatacaggg ggggctgcct gtaacgacaa aacttcctgc tcactggtcg gagcactctc
     2461 gcgtgcgtta tgtgtggggt ttagatcttt gggttccttt gggttccgtg atccgttttt
     2521 gggtgtcttt gatggaaaat ttgggtgtct ttggttattt tccatgcagc taagagttct
     2581 gtttttgggt ctgttttgtg ctgaaacata accattttcg gtactgtttt tattaacagc
     2641 accaatttta cccaccttta aagactcccg tttttgggtg tattcaggct cggcaacact
     2701 ttcttctaca ccgataagtc ggtacaccac aatttgcttt gttctgcctt ttctctcacc
     2761 ggtatcaaca attaacccaa tctccatcag gtgtcgtaag ctgtcctgca cagtcttttt
     2821 gttcagttcc gttacttctg ccagtgcaga tacagacggg tatgcacaca aatcggcacc
     2881 gcacatatca gcaagccagg tcaatactga cttactggat gaactgccgg ttttcacctt
     2941 tttagcccat cgtagtgcat cgatactcat acgaacccct ggcagacatt tgtttatctg
     3001 caaagtaata ttggtattgc tgacgatacg cgtgcttgaa agcaatagct ttttctataa
     3061 gctcgtcagt ctcacgttcc acaacaactg gatccgcaaa aagcagcccg gactccacca
     3121 catcgccata ttctttgttt aacccggcga tcatgtacgt aatgcttttt ccgtcagtaa
     3181 tttcacaata caatctgaaa tcgctgatcc ggatagcctc cataattgcc ggaatcagcg
     3241 ccgtgaattt gtcacgctta tctctggtgt cgatagcttt ccagcgttcg aatatcttca
     3301 cccggttaac gcccagcgcc cgttgatcaa cctcgccatc attaaacgtg acgcgttgaa
     3361 catcgatgtt cgggcgttct ttcagagccc agaatgcttc cgtgattaat atcgtcgcct
     3421 gctcctgtgt cattcctggt cggcataccc aggtatccag agcctcacaa acctgttcag
     3481 tggtgatttt cattgttcaa ccgccccgcc cgctttgcct tacgatattc gtcataaact
     3541 ttggggtcgt actgaagttc cccgccggat gcctcttgca ggcgcatcgc gcgaccttca
     3601 ggaaccagtt ccccccatgc agcaacgctt gccagcctaa ctcctgcgac attggcaagc
     3661 tttgttttgc tgccaaaaaa cgctatagca tcaattttca acatatcgaa ccccttagat
     3721 tttcctaagg aaactagatc gtagagaaac ctaagtcaag aaaaattaga attacctaat
     3781 atgaaaaacg aaaccttcgg tgctcgcctc ttatacaggc gaaaaaaatt aaaactgtct
     3841 caggccgcat taggtaagct ggtcaaagtg gctcacgtaa caatttctca atgggaaaga
     3901 gatgaaacac agccagcggg gaagagatta ttcgcactga gccaggcgct tcagtgctcg
     3961 ccgacttggc ttctttttgg agatgaagat aagcaaccag gcgaaccgat cccagataat
     4021 cagccagtca atctgacaga agatcaaaaa gagttgcttc aactgttcga cgcactgcct
     4081 gagtcagagc aaaaggctct gttgtcagag atgcgtgctc gagttgagaa tttcaacaaa
     4141 ctttttgaag aactactcaa agctcgcaaa agaagcgcaa ataaataacc cccctttttt
     4201 tcgcaactct ctgtaataaa aagcacaaac tttcaaatac ttgtgttttt tacatcaaga
     4261 agcttaggtt tttctacaca aaaagcttga ccataactct taggctattc taaattctac
     4321 tcatcaagac accgcacggt gttctcagca aacagttccg ctacccggcg ttaaggggaa
     4381 gcagaggatt tctcagtggg cgaagtcaaa catcagaatg gaaggcgtcc agggatcagc
     4441 aaagaaacag cgatggcgct ttatattgat atcagcgcca ttgccggaca ggtaagagtt
     4501 atcagagcgg taactaagcg gtatgcgcct ttacttcaga aagtctctgg tgagtgcacc
     4561 gaagatattg tcaacgattt cgtcatcgaa ctgcgaggac tcatcttcag ttacaaggtg
     4621 accacaattt ttgcagatgg ctcccgcgaa actgtcagag ccctgcggct taaaggatgt
     4681 gtcaaagact tagccaccac attctgggca agaaaacttg attgtattca taaccaattt
     4741 cctctcgagt aacagacccc tcagaggata ccacctcgcc tgacgtggtt aaaaagcagg
     4801 caacgctaac cacaaggagc cgacatgcag aaacgagacc ccgtcatcat cgcgccagac
     4861 tataccgatg atgaacttta tgagtggatg caccagaaaa ttaaggcggt gcaggacctg
     4921 aaatgggcca atgaagccag ggctaagcag gctgaaaatc tgtccgctct ggagcaggat
     4981 atcaccaatc tggaaaaagc agcggcatta agcattgcca gaatgattac atacccacgt
     5041 taatagctaa ccaacgaggc taataatgga atttaaagat ttaccaacgc cattacagga
     5101 aatggcatcg aatatagttc gttcacaact ggctactctt gaccttagta ccgcagaaaa
     5161 agaaaccatc gataatatgg ttcgtaatgt gcgcaatgct ttttctgggc tatatggttc
     5221 tgataatcaa aagcaggaaa gcgatgttaa taaacgggta atttctgttt gcgtgaatgg
     5281 ccatgttctt tcatcaatca aaacagaaac ggcgacagtc ttcgattgcc tttgcattgt
     5341 acagagcctt gttgatgccc tgtttcgttc agtgaattta gaaaataatg caaatctgcg
     5401 agggcgcata atagcacatc catatgcaca tactttaggc tctgtggata tcaaagatcc
     5461 cacaaatctt taatgaaata gttaacgcga attgtacttg ctctttcggt tgctttcaga
     5521 atacgcgttg aaactgctgg cggtaatttg gtattccatt tattaaaatc atgcccggga
     5581 aagtactctt cgaaaatact tttaactgca gactcgccta ttgaaatgct gcttaccatg
     5641 cgattttgat aaaggcattt agcaataaga gttgatttta acattcaccc tcctgagggt
     5701 tggtaattaa ggagttctcc acgggtgaag tggagtgcgt gcgccggaca cgggtgaaca
     5761 tccggcactg acagtttact gaaaggatat ttctctgaaa agtcagacca taacgcgaaa
     5821 gcgcacggcg aggtagctgg ttcatagata gcctgtcgtt aaattttcgt cgaccgtgcg
     5881 cttccggttg tggcaatccg cgaaatggcg cggcggtaag tatggagggg ttattccttc
     5941 cccgttgagg acaccgggtt gtcaggttga ccatacgctt aagtgacaac cccgctgcaa
     6001 cgccctctgt tatcaatttt ctggtgacgt ttggcggtat cagttttact ccgtgactgc
     6061 tctgccgccc tttttaaagt gaattttgtg atgtggtgaa tgcggctgag cgcacgcgga
     6121 acagttaaaa ccaaaaacag tgttatgggt ggattctctg tatccggcgt taattgttaa
     6181 ctggttaacg tcacctggag gcaccaggca ccgcatcaca aaattcattg ttgaggacgc
     6241 gataatggaa acgttattac caaacgttaa tacgtctgaa ggttgttttg atattggtgt
     6301 tctgctcagt aaccgggagt ttacggaaga tgccattaag atgagaaaat atgaacctta
     6361 tcttctcaat gataattcca tactttccag aattgccctt cttgaacttg gtattatcgg
     6421 agaacagcag tgacttcagc atttgcactg gtgatgaccg tttttcttat aacgggtgag
     6481 ccacaaaatg tgattaccgg aatttatgac agtaagtcat cctgcattca ggtaagggac
     6541 gaacaaaaaa tccccggtga atgcctcccg ttaaaaaaag tatcgctgaa cctgaataac
     6601 gaaataccgg ctggataacc cgccagccat attaacgcca taccaacgga ttaaaaatgc
     6661 cagcaatggc agggattcgt tcaccctgaa atctgtaatg aggttaaaac aaaatgagta
     6721 aagtctttat ttgcgccgcc attccggacg aacaggcaat aaaggaagaa ggtgccgtc
//
LOCUS       DAZKHF020000234         6758 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2201S31241
            SAMN43267800-rid25106413.denovo.236, whole genome shotgun sequence.
ACCESSION   DAZKHF020000234 DAZKHF020000000
VERSION     DAZKHF020000234.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267800
            Sequence Read Archive: SRR30307263
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6758)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6758)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 6758)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350581.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350581.2
            Genome Coverage       :: 151x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:21:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,652
            CDSs (total)                      :: 5,557
            Genes (coding)                    :: 5,320
            CDSs (with protein)               :: 5,320
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 237
            CDSs (without protein)            :: 237
            Pseudo Genes (ambiguous residues) :: 0 of 237
            Pseudo Genes (frameshifted)       :: 102 of 237
            Pseudo Genes (incomplete)         :: 130 of 237
            Pseudo Genes (internal stop)      :: 52 of 237
            Pseudo Genes (multiple problems)  :: 41 of 237
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6758
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267800-rid25106413.denovo.236"
                     /serotype="O 26:H11"
                     /isolate="2201S31241"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-01"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            <1..166
                     /gene="xisR"
                     /locus_tag="ACHOSC_RS19435"
                     /old_locus_tag="pgaptmp_003887"
     CDS             <1..166
                     /gene="xisR"
                     /locus_tag="ACHOSC_RS19435"
                     /old_locus_tag="pgaptmp_003887"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000005552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="excisionase family protein"
                     /protein_id="HGU4549730.1"
                     /translation="LKKAREKSFMEGREYKHVAHDGMPWDNSPCFYNLEEIDRWIERQ
                     ASARPRRHLT"
     gene            201..1481
                     /locus_tag="ACHOSC_RS19440"
                     /old_locus_tag="pgaptmp_003888"
     CDS             201..1481
                     /locus_tag="ACHOSC_RS19440"
                     /old_locus_tag="pgaptmp_003888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001686441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HGU4549731.1"
                     /translation="MKYPTGVENHGGKLRIWFVYKGVRVRENLGVPDTSKNRRVAGEL
                     RSSVCYAIKTGVFDYAKQFPSSRNLEKFGEARQDLTIKELAEKFLALKETEVAKTSLN
                     TYRAVIKNILSIIGEKNLASSINKEKLLEVRKELLTGYQIPKSNYIVTQPGRSAVTVN
                     NYMTNLNAVFQFGVDNGYLADNPFKGISPLKESRTIPDPLSREEFIRLIDACRNQQAK
                     NLWCVSVYTGVRPGELCALGWEDIDLKNGTMMIRRNLAKDRFTVPKTQAGTNRVIHLI
                     KPAIDALRSQMTLTRLSKEHIIDVHLREYGRTEKQKCTFVFQPEVSARVKNYGDHFTV
                     DSIRQMWDAAIKRAGLRHRKSYQSRHTYACWSLTAGANPAFIANQMGHADAQMVFQVY
                     GKWMSENNNAQVALLNTQLSKFAPTMPHNEAMKN"
     gene            complement(1669..2688)
                     /gene="rspB"
                     /locus_tag="ACHOSC_RS19445"
                     /old_locus_tag="pgaptmp_003889"
     CDS             complement(1669..2688)
                     /gene="rspB"
                     /locus_tag="ACHOSC_RS19445"
                     /old_locus_tag="pgaptmp_003889"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Zn-dependent oxidoreductase"
                     /protein_id="HGU4549732.1"
                     /translation="MKSILIEKPNQLSIIEREIPTPSAGEVRVKVKLAGICGSDSHIY
                     RGHNPFAKYPRVIGHEFFGVIDAVGEGVESARVGERVAVDPVVSCGHCYPCSIGKPNV
                     CTTLAVLGVHADGGFSEYAVVPAKNAWKIPEAVADQYAVMIEPFTIAANVTGHGQPTE
                     NDTVLVYGAGPIGLTIVQVLKGVYNVKNVIVADRIDERLEKAKESGADWAINNSQTPL
                     GEIFAEKGIKPTLIIDAACHPSILKEAVTLASPAARIVLMGFSSEPSEVIQQGITGKE
                     LSIFSSRLNANKFPVVIDWLSKGLIKPEKLITHTFDFQHVADAISLFEQDQKHCCKVL
                     LTFSE"
     gene            complement(2700..3914)
                     /gene="rspA"
                     /locus_tag="ACHOSC_RS19450"
                     /old_locus_tag="pgaptmp_003890"
     CDS             complement(2700..3914)
                     /gene="rspA"
                     /locus_tag="ACHOSC_RS19450"
                     /old_locus_tag="pgaptmp_003890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001625315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="starvation-sensing protein RspA"
                     /protein_id="HGU4549733.1"
                     /translation="MKIVKAEVFVTCPGRNFVTLKITTEDGITGLGDATLNGRELSVA
                     SYLQDHLCPQLIGRDAHRIEDIWQFFYKGAYWRRGPVTMSAISAVDMALWDIKAKAAN
                     MPLYQLLGGASREGVMVYCHTTGHSIDEALDDYARHQELGFKAIRVQCGIPGMKTTYG
                     MSKGKGLAYEPATKGQWPEEQLWSTEKYLDFMPKLFDAVRNKFGFNEHLLHDMHHRLT
                     PIEAARFGKSIEDYRMFWMEDPTPAENQECFRLIRQHTVTPIAVGEVFNSIWDCKQLI
                     EEQLIDYIRTTLTHAGGITGMRRIADFASLYQVRTGSHGPSDLSPVCMAAALHFDLWV
                     PNFGVQEYMGYSEQMLEVFPHNWTFDNGYMHPGDKPGLGIEFDEKLAAKYPYEPAYLP
                     VARLEDGTLWNW"
     gene            complement(4120..4446)
                     /gene="ynfA"
                     /locus_tag="ACHOSC_RS19455"
                     /old_locus_tag="pgaptmp_003891"
     CDS             complement(4120..4446)
                     /gene="ynfA"
                     /locus_tag="ACHOSC_RS19455"
                     /old_locus_tag="pgaptmp_003891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YnfA family protein"
                     /protein_id="HGU4549734.1"
                     /translation="MIKTTLLFFATALCEIIGCFLPWLWLKRNASIWLLLPAGISLAL
                     FVWLLTLHPAASGRVYAAYGGVYVCTALMWLRVVDGVKLTLYDWTGALIALCGMLIIV
                     AGWGRT"
     gene            4581..4922
                     /gene="ynfB"
                     /locus_tag="ACHOSC_RS19460"
                     /old_locus_tag="pgaptmp_003892"
     CDS             4581..4922
                     /gene="ynfB"
                     /locus_tag="ACHOSC_RS19460"
                     /old_locus_tag="pgaptmp_003892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1283 family protein"
                     /protein_id="HGU4549735.1"
                     /translation="MKITLSKRIGLLAILLPCALALSTTVHAETNKLVIESGDSAQSR
                     QHAAMEKEQWNDTRNLRQKVNKRTEKEWDKADAAFDNRDKCEQSANINAYWEPNTLRC
                     LDRRTGRVITP"
     gene            4957..5517
                     /gene="speG"
                     /locus_tag="ACHOSC_RS19465"
                     /old_locus_tag="pgaptmp_003893"
     CDS             4957..5517
                     /gene="speG"
                     /locus_tag="ACHOSC_RS19465"
                     /old_locus_tag="pgaptmp_003893"
                     /EC_number="2.3.1.57"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="spermidine N1-acetyltransferase"
                     /protein_id="HGU4549736.1"
                     /translation="MPSAHSVKLRPLEREDLRYEHQLDNNASVMRYWFEEPYEAFVEL
                     SDLYDKHIHDQSERRFVVECDGEKAGLVELVEINHVHRRAEFQIIISPEYQGKGLATR
                     AAKLAMDYGFTVLNLYKLYLIVDKENEKAIHIYRKLGFSVEGELMHEFFINGQYRNAI
                     RMCIFQHQYLAEHKTPGQTLLKPTAQ"
     gene            complement(5520..6230)
                     /gene="ynfC"
                     /locus_tag="ACHOSC_RS19470"
                     /old_locus_tag="pgaptmp_003894"
     CDS             complement(5520..6230)
                     /gene="ynfC"
                     /locus_tag="ACHOSC_RS19470"
                     /old_locus_tag="pgaptmp_003894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416102.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YnfC family lipoprotein"
                     /protein_id="HGU4549737.1"
                     /translation="MKYKLLPCLLAIFLTGCDRTEVTLSFTPEMASFSNEFDFDPLRG
                     PVKDFTQTLMDEQGEVTKRVSGTLSEEGCFDSLELLDLENNTVVALVLDANYYRDAET
                     LEKRVRLQGKCQLAELPSAGVSWETDDNGFVIKASSKQMQMEYRYDDQGYPLGKTTKS
                     NDKTLSVSATPSTDPIKKLDYTAVTLLNNQRVGNVKQSCEYDSHANPVDCQLIIVDEG
                     VKPAVERVYTIKNTIDYY"
     gene            6338..6643
                     /gene="ynfD"
                     /locus_tag="ACHOSC_RS19475"
                     /old_locus_tag="pgaptmp_003895"
     CDS             6338..6643
                     /gene="ynfD"
                     /locus_tag="ACHOSC_RS19475"
                     /old_locus_tag="pgaptmp_003895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707474.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1161 domain-containing protein"
                     /protein_id="HGU4549738.1"
                     /translation="MKLSTCCAALLLALASPVVLAAPGSCERIQSDISQRIINNGVPE
                     SSFTLSIVPNDQVDQPDSQVVGHCANDTHKILYTRTTSGNVSAPAQSTQDGAPAEPQ"
BASE COUNT         1941 a         1632 c         1471 g         1714 t
ORIGIN      
        1 gttaaaaaag gcccgggaaa aatcgtttat ggaaggaagg gaatataagc atgtcgctca
       61 tgacggtatg ccatgggata acagtccatg cttttataac ctggaagaaa ttgatcgctg
      121 gattgagcgc caggcatcag cgagaccaag acgtcatctt acttgactaa aagccacact
      181 aactaatgag agaagttgaa atgaaatatc cgacaggcgt ggaaaaccat ggagggaaat
      241 tacgtatctg gtttgtttat aaaggcgtaa gagtcaggga aaatctgggg gttcctgaca
      301 catcaaaaaa caggcgcgtt gcaggtgaac tacgctcctc tgtttgttac gcaataaaaa
      361 ctggtgtttt cgactatgca aaacagtttc cctcctcacg caatctggaa aaatttggtg
      421 aggcccgaca agatttaacc ataaaagaac tggctgaaaa atttttggca ctgaaagaaa
      481 ctgaagtcgc caaaacatca ctcaacacat accgtgccgt catcaaaaat atcctgagca
      541 taatcggtga aaaaaatctt gcctcatcga ttaataaaga aaaattactg gaggttcgta
      601 aagagctact gactggatac cagatcccca aaagtaacta tattgttaca caaccaggga
      661 gatcggctgt aactgtaaat aattacatga caaatcttaa cgccgtgttc cagtttggtg
      721 ttgataacgg ttacctggca gataatccgt ttaaggggat ctcgccatta aaggaatcaa
      781 gaaccattcc ggatcctctt tcgcgggagg aatttatccg tcttatcgat gcgtgcagaa
      841 atcagcaagc aaaaaattta tggtgtgttt ctgtttatac tggcgttcgc cctggtgagc
      901 tgtgtgcact tggatgggag gacatagatc tgaaaaatgg aacaatgatg atcaggagaa
      961 atttagcaaa agaccgtttc acggtaccaa aaacacaggc gggaaccaat cgggtcattc
     1021 atcttattaa gccagcaatc gacgctctcc ggagtcagat gacattaacg agactgagca
     1081 aagagcatat cattgatgtt cacctcagag agtatggcag aacagaaaaa caaaaatgca
     1141 cctttgtttt tcaacctgaa gtgtcagcga gagtaaaaaa ttatggtgac cattttaccg
     1201 ttgactcaat aaggcagatg tgggacgcag cgataaaacg tgccggactc cgccatcgaa
     1261 aatcatatca gtcgagacat acttatgcct gctggtcgct gacagctggt gctaacccgg
     1321 catttatagc aaaccagatg ggccatgcag atgcgcaaat ggtatttcag gtatacggaa
     1381 aatggatgtc tgaaaacaat aatgcacagg tagctttgtt aaatacacag ttaagcaagt
     1441 ttgccccaac catgccccat aacgaagcaa tgaaaaatta atttaatatt tatcaaatag
     1501 ttaacacgca tgactcttga aatccataaa ttcaagcgca gtgcccagcc atcccgatac
     1561 tgctgctttc accaaatcct tagtgcttct ttcatgtttt tctattgtca taatggttat
     1621 ctctaaaaaa gaggtaagat gcgtactact tactcgccgt tattggtatt attcagaaaa
     1681 agtgagcaag actttgcagc aatgtttttg atcctgttca aataaactaa tggcatcagc
     1741 aacatgctgg aaatcaaacg tatgggtaat taatttttct ggtttaatta accctttact
     1801 taaccagtcg ataacaaccg gaaatttatt tgcatttaag cgtgaagaga aaatagagag
     1861 ttcttttccg gtaattcctt gctgaatcac ttcagacggt tcactggaga agcccatcaa
     1921 tacaatacgt gccgctggag aagccagcgt tacggcctct ttcaggatag aaggatgaca
     1981 agccgcatcg ataattaatg tcggcttgat gcctttttca gcgaaaatct cgccaagcgg
     2041 tgtctggctg ttattaatcg cccagtctgc cccgctctct ttcgcttttt ccagtcgttc
     2101 atcaatgcga tcggcaacaa tcacattttt aacgttatag acgcctttta atacctgaac
     2161 gatcgtcagg ccgattggac cggcaccata aaccagaacg gtatcatttt cagtcggttg
     2221 accatgaccg gtaacgttag ccgcaatggt aaaaggttcg atcatcaccg catattgatc
     2281 ggccactgct tcaggaattt tccacgcatt ttttgccgga accacggcat attcactgaa
     2341 accaccgtca gcgtgcacac ctaatacagc aagtgtcgta caaacgttcg gcttacctat
     2401 agagcacgga tagcaatgcc cacagctgac caccggatcg acagcaacgc gctcaccgac
     2461 tctggcgctt tccacacctt cacccaccgc atcaatgacg ccaaagaatt catgaccaat
     2521 gacgcgcgga tatttcgcaa aaggattatg cccacggtaa atatggctat ctgaaccaca
     2581 aattccggca agtttcactt ttactcgtac ttcacccgct gacggggtgg gtatttcacg
     2641 ttcgataatc gacagttgat tcggtttttc aattaaaatg cttttcatta ccttactcct
     2701 taccagttcc acagcgtgcc atcttccaga cgtgcgactg gcagataagc aggttcatag
     2761 ggatatttcg ccgccagctt ttcatcgaat tcgatgccaa gacccggttt gtctcccgga
     2821 tgcatatagc cgttatcgaa agtccagttg tgcgggaaga cttcgagcat ttgttcggaa
     2881 taacccatgt actcctggac accgaaattg gggacccaca gatcaaagtg cagcgccgca
     2941 gccatgcaga ctggtgacaa atcggaagga ccgtgtgagc cagtacgtac ctgatacagc
     3001 gaagcaaaat cggcaatccg gcgcataccg gtaattccgc ctgcatgggt cagcgtggtg
     3061 cggatataat cgatgagttg ctcttcaatc agttgtttgc agtcccagat gctgttgaag
     3121 acttcaccca ctgcgatggg tgtgacggta tgttggcgaa tgagacggaa gcattcctgg
     3181 ttttccgcag gcgtcgggtc ttccatccag aacatgcgat aatcttcaat gcttttacca
     3241 aagcgcgccg cttcaatagg cgttaagcga tggtgcatgt catgcagcaa atgttcatta
     3301 aaaccaaact tgttacgtac cgcgtcaaac aatttcggca tgaaatcgag gtatttctcc
     3361 gtcgaccaca gctgctcttc cggccactgt cctttggttg cgggttcata agccagacct
     3421 ttacctttcg acatgccgta ggtggttttc ataccaggga ttccgcactg cacgcggatg
     3481 gctttgaatc ccagctcctg atgacgggca taatcatcca gagcttcatc aatactgtga
     3541 ccggtggtat ggcaataaac catcacccct tcacgagacg cgccgccgag taactggtaa
     3601 agcggcatgt tggcagcttt ggctttaata tcccacagcg ccatatcaac cgctgaaatg
     3661 gccgacatcg taaccggacc gcgacgccag taagcacctt tatagaaaaa ctgccagata
     3721 tcttcgatac ggtgcgcatc gcgaccaata agctgcggac aaaggtgatc ctgcaaataa
     3781 gaggccacgg aaagctcacg tccattgagg gtggcatccc caaggcccgt aataccgtcc
     3841 tcagtggtga tttttaatgt gacgaaatta cgccccggac aggtaacaaa aacttcagcc
     3901 tttacgatct tcatgttcga ttccttgcat cgcttgtcgt gatgcatgaa atctacgcaa
     3961 ctgagctact accatacaag cataaagatc gaaaaaagcc ggagtgatca caaaaaaagg
     4021 cgtatatttg cgctgtgaat ggttgacaaa agatgaaata gaataccttt tgtcagctga
     4081 cacttcctct tatcttattg ataaaatgga tttatgttcc tacgtgcgcc cccagcccgc
     4141 aacaatgatc aacatgccgc aaagcgcaat caacgcaccc gtccagtcat aaagagtcag
     4201 tttcacgcca tccacaacgc gcagccacat caacgccgtg cagacataaa cgccaccata
     4261 agccgcgtaa acacgcccac tcgccgctgg atgcaacgtt aacaaccaga caaacagcgc
     4321 cagtgaaatc cccgccggaa gcaacagcca gatactggcg tttcgtttta accacaacca
     4381 gggcagaaag catccaataa tttcacacag cgcagtagca aaaaatagta acgttgtttt
     4441 aatcatcttt gtctcttatt gacatcatgt atagttatag ggcgacataa tatcatcaat
     4501 ataaacaccc tcctggtacg ttttgcgtcc gcagtggatg gtgtagaatc acctttaatc
     4561 attcatacag ggaatgaatt atgaaaatca ctctcagcaa acgaatcggc ctgctcgcta
     4621 ttctgctgcc ttgcgcactg gcattgagca caactgttca tgccgaaact aacaaactgg
     4681 tgattgagtc tggcgacagt gcacaaagcc gccagcacgc cgctatggaa aaagagcaat
     4741 ggaatgacac gcgcaatctg cgccagaaag tgaataaacg cactgaaaaa gagtgggata
     4801 aagccgacgc cgcttttgat aaccgcgata aatgtgagca aagcgccaac atcaatgcct
     4861 actgggagcc caatactttg cgctgcctgg accgtcgaac tggccgcgtt attaccccct
     4921 aacctgttat tgatttaagg aatgtaagga cacgttatgc caagcgccca cagtgttaag
     4981 ctacgcccgc tggagcgtga agatttacgc tatgaacatc aactcgacaa caacgccagt
     5041 gtgatgcgtt actggtttga ggaaccctac gaagcctttg ttgaactctc tgatctgtat
     5101 gataagcata ttcacgatca gagcgaacgg cgctttgtgg tggaatgtga cggcgaaaaa
     5161 gccggtctgg tggagctggt ggaaattaac catgttcatc gccgcgcaga atttcagata
     5221 attatctccc cggagtatca ggggaaaggt ctggcaaccc gtgccgccaa attagcaatg
     5281 gactatggct ttaccgttct caatctctat aagctgtatc tgatcgttga taaagagaat
     5341 gaaaaagcga ttcacattta ccgcaagctt ggcttttcgg ttgaaggtga attgatgcac
     5401 gagttcttta ttaatggtca atatcgtaat gccattcgca tgtgtatatt ccagcatcag
     5461 tatctggcag agcacaaaac accgggtcag actctcctga agccgaccgc acaatagcat
     5521 taataataat cgatcgtatt tttgatggtg taaacccgtt cgacggcggg ttttactcct
     5581 tcatcaacaa tgattagctg acagtccacc ggattagcgt gactgtcata ttcacagctc
     5641 tgttttacat taccaacccg ttgattattc agtaaagtaa ccgctgtgta atctaatttt
     5701 ttgatcggat ccgttgatgg cgtggcgctg acagataatg ttttgtcgtt acttttcgtg
     5761 gttttaccca gcggataacc ctgatcatca tagcgatatt ccatctgcat ttgtttgctg
     5821 ctggctttaa tcacgaagcc attatcatcg gtttcccaac tcaccccggc agaaggtaat
     5881 tctgctagct ggcattttcc ctgtaaacgt actctcttct ccagcgtctc ggcatcacgg
     5941 taataattgg cgtccagtac cagagcgacc acggtattat tttccagatc cagtaattcg
     6001 agtgaatcaa aacagccttc ttccgacaaa gtcccagaaa cacgtttcgt cacttcacct
     6061 tgctcatcca ttaatgtctg agtgaaatct tttaccggac cacgcagcgg atcaaaatcg
     6121 aattcattag agaaactggc catctcaggg gtaaatgaaa gtgttacctc tgtgcggtca
     6181 catcctgtga ggaatatcgc gagtaagcat ggtagtaatt tgtatttcac aacagtcacc
     6241 agagagtaga gatgattctc aatcatagta gcaaatacag tactttacac gttaaatgct
     6301 atgcttaaag aagttatctt cgcgtaagga gcttatgatg aaactctcaa cctgctgtgc
     6361 cgcgcttctg ctcgccctcg cctcacccgt ggtactcgcc gcgccgggat cctgtgagcg
     6421 catacagagc gatatatcac agcgcattat caataatggc gtacccgaaa gcagcttcac
     6481 gttaagtatt gtacccaatg accaggttga tcagcctgat tcccaggtcg tcggccattg
     6541 cgctaatgat acgcataaaa ttctctatac ccgcaccacc agcggtaacg tctctgctcc
     6601 cgcgcagtct acccaggatg gcgcgcctgc cgaaccgcag taatacatta tcgactgaac
     6661 gccggatacg acacatccgg cgatttgaac atacaacata atcccacctt attactcata
     6721 cccttctatt gatatggatt aataattctt aacccaaa
//
LOCUS       DAZKHF020000235         6733 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2201S31241
            SAMN43267800-rid25106413.denovo.237, whole genome shotgun sequence.
ACCESSION   DAZKHF020000235 DAZKHF020000000
VERSION     DAZKHF020000235.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267800
            Sequence Read Archive: SRR30307263
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6733)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6733)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 6733)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350581.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350581.2
            Genome Coverage       :: 151x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:21:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,652
            CDSs (total)                      :: 5,557
            Genes (coding)                    :: 5,320
            CDSs (with protein)               :: 5,320
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 237
            CDSs (without protein)            :: 237
            Pseudo Genes (ambiguous residues) :: 0 of 237
            Pseudo Genes (frameshifted)       :: 102 of 237
            Pseudo Genes (incomplete)         :: 130 of 237
            Pseudo Genes (internal stop)      :: 52 of 237
            Pseudo Genes (multiple problems)  :: 41 of 237
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6733
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267800-rid25106413.denovo.237"
                     /serotype="O 26:H11"
                     /isolate="2201S31241"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-01"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            complement(178..1107)
                     /gene="fdhE"
                     /locus_tag="ACHOSC_RS19480"
                     /old_locus_tag="pgaptmp_003896"
     CDS             complement(178..1107)
                     /gene="fdhE"
                     /locus_tag="ACHOSC_RS19480"
                     /old_locus_tag="pgaptmp_003896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase accessory protein FdhE"
                     /protein_id="HGU4549739.1"
                     /translation="MSIRIIPQDELGSSEKRTADMIPPLLFPRLKNLYNRRAERLREL
                     AENNPLGDYLRFAALIAHAQEVVLYDHPLEMDLTARIKEASAQGKPPLDIHVLPRDKH
                     WQKLLMALIAELKPEMSGPALAVIENLEKASTQELEDMASALFASDFSSVSSDKAPFI
                     WAALSLYWAQMANLIPGKARAEYGEQRQYCPVCGSMPVSSMVQIGTTQGLRYLHCNLC
                     ETEWHVVRVKCSNCEQSGKLHYWSLDDEQAAIKAESCDDCGTYLKILYQEKEPKVEAV
                     ADDLASLVLDARMEQEGYARSSINPFLFPGEGE"
     gene            complement(1104..1739)
                     /gene="fdoI"
                     /locus_tag="ACHOSC_RS19485"
                     /old_locus_tag="pgaptmp_003897"
     CDS             complement(1104..1739)
                     /gene="fdoI"
                     /locus_tag="ACHOSC_RS19485"
                     /old_locus_tag="pgaptmp_003897"
                     /EC_number="1.17.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase cytochrome b556 subunit"
                     /protein_id="HGU4549740.1"
                     /translation="MKRRDTILRYTAPERINHWITAFCFILAAVSGLGFLFPSFNWLM
                     QIMGTPQLARILHPFVGVVMFASFIIMFFRYWHHNLINRDDIFWAKNIRKIVVNEEVG
                     DTGRYNFGQKCVFWAAIIFLVLLLVSGVIIWRPYFAPAFSIPVIRFALMLHSFAAVAL
                     IVVIMVHIYAALWVKGTITAMVEGWVTSAWAKKHHPRWYREVRKTTEKKAE"
     gene            complement(1736..2638)
                     /gene="fdoH"
                     /locus_tag="ACHOSC_RS19490"
                     /old_locus_tag="pgaptmp_003898"
     CDS             complement(1736..2638)
                     /gene="fdoH"
                     /locus_tag="ACHOSC_RS19490"
                     /old_locus_tag="pgaptmp_003898"
                     /EC_number="1.17.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase O subunit beta"
                     /protein_id="HGU4549741.1"
                     /translation="MAYQSQDIIRRSATNGLTPAPQARDFQEEVAKLIDVTTCIGCKA
                     CQVACSEWNDIRDTVGNNIGVYDNPNDLSAKSWTVMRFSEVEQNDKLEWLIRKDGCMH
                     CSDPGCLKACPAEGAIIQYANGIVDFQSEQCIGCGYCIAGCPFDIPRLNPEDNRVYKC
                     TLCVDRVVVGQEPACVKTCPTGAIHFGTKESMKTLASERVAELKTRGYDNAGLYDPAG
                     VGGTHVMYVLHHADKPNLYHGLPENPEISETVKFWKGIWKPLAAVGFAATFAASIFHY
                     VGVGPNRADEEENNLHEEKDEERK"
     gene            complement(2651..5701)
                     /gene="fdnG"
                     /locus_tag="ACHOSC_RS19495"
                     /old_locus_tag="pgaptmp_003899"
     CDS             complement(2651..5701)
                     /gene="fdnG"
                     /locus_tag="ACHOSC_RS19495"
                     /old_locus_tag="pgaptmp_003899"
                     /EC_number="1.17.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009326 - formate dehydrogenase complex
                     [Evidence IEA];
                     GO_function: GO:0008863 - formate dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0043546 - molybdopterin cofactor binding
                     [Evidence IEA];
                     GO_function: GO:0047111 - formate dehydrogenase
                     (cytochrome-c-553) activity [Evidence IEA];
                     GO_process: GO:0015942 - formate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_except=(pos:complement(5114..5116),aa:Sec)
                     /transl_table=11
                     /product="formate dehydrogenase-N subunit alpha"
                     /protein_id="HGU4549742.1"
                     /translation="MQVSRRQFFKICAGGMAGTTAAALGFAPSVALAETRQYKLLRTR
                     ETRNTCTYCSVGCGLLMYSLGDGAKNAKASIFHIEGDPDHPVNRGALCPKGAGLVDFI
                     HSESRLKFPEYRAPGSDKWQQISWEEAFDRIAKLMKEDRDANYIAQNAEGVTVNRWLS
                     TGMLCASASSNETGYLTQKFSRALGMLAVDNQARVUHGPTVASLAPTFGRGAMTNHWV
                     DIKNANLVVVMGGNAAEAHPVGFRWAMEAKIHNGAKLIVIDPRFTRTAAVADYYAPIR
                     SGTDIAFLSGVLLYLLNNEKFNREYTEAYTNASLIVREDYGFEDGLFTGYDAEKRKYD
                     KSSWTYELDENGFAKRDTTLQHPRCVWNLLKQHVSRYTPDVVENICGTPKDAFLKVCE
                     YIAETSAHDKTASFLYALGWTQHSVGAQNIRTMAMIQLLLGNMGMAGGGVNALRGHSN
                     IQGLTDLGLLSQSLPGYMTLPSEKQTDLQTYLTANTPKPLLEGQVNYWGNYPKFFVSM
                     MKAFFGDKATAENSWGFDWLPKWDKGYDVLQYFEMMKEGKVNGYICQGFNPVASFPNK
                     NKVIGCLSKLKFLVTIDPLNTETSNFWQNHGELNEVDSSKIQTEVFRLPSTCFAEENG
                     SIVNSGRWLQWHWKGADAPGIALTDGEILSGIFLRLRKMYAEQGGANPDQVLNMTWNY
                     AIPHEPKSEEVAMESNGKALADITDPATGAVIVKKGQQLSSFAQLRDDGTTSCGCWIF
                     AGSWTPEGNQMARRDNADPSGLGNTLGWAWAWPLNRRILYNRASADPQGNPWDPKRQL
                     LKWDGTKWTGWDIPDYSAAPPGSGVGPFIMQQEGMGRLFALDKMAEGPFPEHYEPFET
                     PLGTNPLHPNVISNPAARIFKDDAEALGKADKFPYVGTTYRLTEHFHYWTKHALLNAI
                     LQPEQFVEIGESLANKLGIAQGDTVKVSSNRGYIKAKAVVTKRIRTLKANGKDIDTIG
                     IPIHWGYEGVAKKGFIANTLTPFVGDANTQTPEFKSFLVNVEKV"
     gene            5895..6728
                     /gene="fdhD"
                     /locus_tag="ACHOSC_RS19500"
                     /old_locus_tag="pgaptmp_003900"
     CDS             5895..6728
                     /gene="fdhD"
                     /locus_tag="ACHOSC_RS19500"
                     /old_locus_tag="pgaptmp_003900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009326 - formate dehydrogenase complex
                     [Evidence IEA];
                     GO_function: GO:0016783 - sulfurtransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009061 - anaerobic respiration [Evidence
                     IEA];
                     GO_process: GO:0015942 - formate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase accessory
                     sulfurtransferase FdhD"
                     /protein_id="HGU4549743.1"
                     /translation="MKKTQQKEIENVTNITGVRQIELWRRDDLQHPRLDEVAEEVPVA
                     LVYNGISHVVMMASPKDLEYFALGFSLSEGIIESPRDIFGMDVVPSCNGLEVQIELSS
                     RRFMGLKERRRALAGRTGCGVCGVEQLNDIGKPVQPLPFTQTFDLNKLDDALRHLNDF
                     QPVGQLTGCTHAAAWMLPSGELVGGHEDVGRHVALDKLLGRRSQEGESWQQGAVLVSS
                     RASYEMVQKSAMCGVEILFAVSAATTLAVEVAERCNLTLVGFCKPGRATVYTHPQRLS
                     N"
BASE COUNT         1468 a         1829 c         1828 g         1608 t
ORIGIN      
        1 atacgcttgt catcagtgac atacccataa ccttatcgat gtgttggagg gaagaacgag
       61 gcaaacagac aattgcggca tgatcttgaa tgggtaacgc ggaatacgag agatggtgtc
      121 gtcacaccct atgagctgta atccgggcga ccagcgccac ccggaaaacg tcagagatta
      181 ctccccttca cccggaaaca ggaacgggtt gatggaactg cgggcatagc cttcttgctc
      241 cattcgcgcg tccagtacca gagaggcgag gtcatctgcc acggcttcaa ctttcggttc
      301 tttttcctga taaagaatct tcaggtaagt gccgcagtca tcgcagcttt cggctttaat
      361 cgcggcctgt tcgtcatcca gcgaccagta atgcagtttg ccgctctgct cgcagttgct
      421 gcattttacg cgcactacgt gccattcggt ttcacacagg ttgcagtgca ggtaacgcag
      481 accctgagtg gtgccaattt gcaccatgct ggacaccggc atagaaccac aaaccgggca
      541 atattgacgt tgctcgccgt attcagcgcg ggctttgccg gggatcagat tggccatctg
      601 cgcccagtag agcgacagtg cagcccagat aaacggcgct ttatcgctgc tgacggatga
      661 gaaatcagag gcaaacagtg cgctggccat atcttccagc tcctgagtcg atgccttctc
      721 cagattctca atcactgcca gcgccgggcc gctcatttca ggtttcagct cagcaatcag
      781 cgccatcagc agcttttgcc agtgcttatc acgcggcaga acgtgaatat ccagcggggg
      841 cttgccctgt gcgctggctt ctttaatgcg cgcggtcaga tccatctcca gcggatggtc
      901 gtacagcacc acttcctggg cgtgggcgat aagcgcagca aagcgcaggt aatcacccag
      961 cggattattt tctgccagct cgcgcagacg ctcggcgcgg cggttgtata aattcttgag
     1021 ccgagggaac aataacggcg gaatcatatc cgccgtacgt ttctcgctcg aacccagctc
     1081 atcttgcggg attatgcgaa tactcattca gctttctttt ccgttgtctt gcggacctca
     1141 cggtaccagc gcgggtgatg tttcttcgcc caggcgctgg taacccatcc ttccaccatg
     1201 gcggtaatcg tgcctttcac ccaaagggcg gcgtagatat gcaccatgat aaccacaatt
     1261 aacgccactg cggcaaatga atgcagcatt aacgcgaatc ggatcaccgg gattgagaaa
     1321 gcaggcgcaa aataaggacg ccagataatc acgccgctca ccagcagcag aaccaggaaa
     1381 ataatcgccg cccagaaaac gcatttctga ccgaagttat aacgcccggt gtcacctact
     1441 tcctcgttga cgacgatctt acgaatattc ttcgcccaaa agatatcatc ccgattgatt
     1501 aggttgtgat gccagtaacg gaaaaacatg atgatgaacg aggcaaacat aaccacgccg
     1561 acaaacgggt gcagaattcg cgccagctgc ggtgtgccca tgatttgcat caaccagttg
     1621 aaggacggga acaaaaagcc cagcccgctc accgccgcca ggatgaagca gaaggcggtg
     1681 atccagtggt tgatacgttc cggcgcggtg tagcgcagga tggtgtcacg tcgtttcatt
     1741 tgcgctcctc gtctttctct tcgtgcagat tattctcttc ctcatccgca cggttcggac
     1801 cgacacccac gtagtggaag atactggccg caaaggtagc cgcaaagcca acagccgcga
     1861 gcggtttcca gatgcctttc cagaatttca cggtttcgct gatttccggg ttctccggca
     1921 agccatgata cagatttggc ttgtcagcat ggtgcagcac gtacattacg tgtgtaccac
     1981 cgacgcctgc cggatcgtac agacccgcat tgtcgtaacc gcgggttttc agctcagcaa
     2041 cgcgctcgct cgccagcgtt ttcatcgact ctttcgtacc gaagtgaatc gcgcccgttg
     2101 ggcaggtctt cacgcaggct ggttcttgcc caaccaccac gcggtcaacg cacagcgtac
     2161 atttgtagac gcggttgtct tccgggttga ggcgcggaat gtcgaacgga cagcccgcaa
     2221 tgcaataacc gcagccaatg cactgctcgg actggaagtc gacgataccg ttggcatact
     2281 gaataattgc accttccgcc gggcacgctt tcaggcagcc tggatcggaa cagtgcatac
     2341 agccgtcttt gcggatcagc cattccagtt tgtcgttctg ctccacttcc gagaagcgca
     2401 ttaccgtcca cgatttggcg cttaaatcat tggggttgtc gtacacccca atgttattgc
     2461 cgacggtatc gcgaatgtcg ttccactctg aacacgccac ctgacaggct ttacagccga
     2521 tacaggtggt gacgtcgatg agtttcgcca cttcttcctg gaagtcccgc gcctgaggcg
     2581 cgggggtcag accgttagtc gcggaacgac gaatgatatc ttgagattga taagccatat
     2641 gtcgtctccg ttacaccttt tccacattca caaggaagga cttaaactcc ggcgtctgcg
     2701 tgttcgcatc accaacgaat ggcgttaacg tattggcaat aaagcctttt ttagctacgc
     2761 cttcatagcc ccagtgaatc ggaataccga tggtatcgat atctttgccg ttcgctttca
     2821 gtgtacgaat acgtttggtc accaccgctt tggccttgat atagccacgg ttggaggaga
     2881 ctttcacggt atcgccctgg gcaatgccaa gtttattcgc cagcgactcc ccgatttcca
     2941 caaactgctc tggttgcaaa atcgcgttca acagcgcgtg tttggtccag tagtggaaat
     3001 gctcggtcag acgataggtg gttccgacat acgggaactt atcggcttta cccaatgctt
     3061 cggcgtcgtc tttaaagata cgtgcagccg ggttcgagat aacgtttgga tgcagcgggt
     3121 tagttcccag cggcgtttca aacggctcgt agtgttccgg gaacggacct tccgccatct
     3181 tatcgagggc aaacagacgc cccatgcctt cctgctgcat gataaacggc ccgacgccgc
     3241 tacccggagg cgctgcgctg tagtccggaa tatcccagcc ggtccactta gtgccgtccc
     3301 atttcagcaa ctgacgcttc ggatcccacg ggttaccctg cggatctgcg gaggcgcggt
     3361 tatacagaat gcggcggtta agcggccatg cccatgccca gcccagcgtg ttaccgaggc
     3421 cagacggatc ggcgttatca cgacgcgcca tctggttgcc ttccggcgtc cagctaccgg
     3481 cgaaaatcca gcagccacag gaggttgtac cgtcatcgcg caattgggcg aacgagctaa
     3541 gttgttggcc tttcttgacg ataaccgcac cggttgccgg atcggtaata tcggccagcg
     3601 ccttaccgtt gctctccatc gctacttctt ccgacttcgg ctcatgcgga atggcgtagt
     3661 tccaggtcat gttcagcacc tgatccgggt tcgcgccacc ctgttcggca tacatcttgc
     3721 gcaagcgcag gaagataccg gagaggatct caccgtcagt cagcgcaatc cccggggcgt
     3781 ccgcaccttt ccagtgccac tgcaaccagc gacctgagtt aacgattgaa ccgttctctt
     3841 ctgcgaagca ggtcgatggc agacggaaca cttcggtctg gatcttcgac gagtcaactt
     3901 cgttcagctc accgtggttc tgccagaagt tagaggtttc agtgttcagc gggtcgatgg
     3961 tgacgaggaa cttcagtttc gacagacaac cgatcacttt gtttttgttc gggaatgagg
     4021 caacagggtt aaagccctgg cagatatagc cattgacctt gccttctttc atcatctcga
     4081 agtattgcag gacgtcgtag cctttatccc acttcggcaa ccagtcaaag ccccagctat
     4141 tttccgccgt cgctttatca ccaaagaagg ccttcatcat agagacgaag aatttcgggt
     4201 agttgcccca gtagtttacc tggccttcca gcagcggttt tggcgtgttg gcggtaaggt
     4261 aggtttgcag atcggtctgc ttctcgcttg gcagcgtcat gtaacctggc agactctgcg
     4321 acagtagccc caggtccgtc agcccctgaa tattggagtg accgcgcagg gcgttaacgc
     4381 cgccgccggc catccccata ttgccgagca gcagctggat catcgccatc gtacgaatgt
     4441 tttgcgcacc aacggagtgt tgcgtccagc cgagggcata caggaacgag gcagttttat
     4501 cgtgagcact ggtttctgcg atgtattcgc agactttcag gaacgcgtct tttggcgtac
     4561 cacagatgtt ttcaaccaca tctggcgtgt agcgggaaac gtgctgtttc agcaagttcc
     4621 acacgcagcg cggatgttgc agcgtggtat cgcgtttggc gaagccgttt tcgtccagtt
     4681 cataagtcca ggaggattta tcgtacttgc gtttttccgc gtcgtagccg gtgaacaggc
     4741 catcttcaaa gccgtaatcc tcacgcacta tcaggctggc gttggtatag gcttcggtgt
     4801 attcgcggtt gaatttttca ttgttcagca ggtacagcaa tacgcctgac aggaaagcaa
     4861 tgtcagtacc ggaacgaata ggggcatagt agtcagctac cgccgccgta cgcgtaaagc
     4921 gaggatcgat cacaatcagc ttcgcgccgt tgtgaatttt ggcttccatc gcccagcgga
     4981 acccgaccgg gtgagcttca gcggcgttac cgcccatcac cacgacgagg ttggcgttct
     5041 tgatgtcgac ccagtggttg gtcatcgcac cgcgaccaaa tgttggagca agacttgcta
     5101 ccgttggtcc gtgtcagaca cgcgcctggt tgtcgaccgc gagcataccc agcgcgcggg
     5161 agaatttttg cgttaaatag ccggtttcgt tactcgacgc ggaagcacac agcatcccgg
     5221 tggagagcca gcggttaaca gtcacgcctt cggcgttttg cgcaatgtag ttagcatcgc
     5281 ggtcttcttt catcagtttg gcgatgcgat caaacgcctc ttcccaactg atttgctgcc
     5341 atttatcaga acctggcgca cggtattccg gaaacttcag acggctttcg gagtggatga
     5401 aatccaccag gccagcgcct ttcggacaaa gtgcaccgcg gttgaccggg tgatccggat
     5461 cgccttcgat atggaagata gatgctttgg cgttttttgc tccgtcaccg aggctgtaca
     5521 tcaacagccc acagcctacg gaacaatagg tgcaggtatt acgggtttcg cgggtgcgca
     5581 gcagtttata ctgccgggtt tccgcgagcg ctacgctggg tgcaaaaccc agtgccgctg
     5641 ccgtggtgcc tgccataccg ccagcgcaga tcttaaagaa ctgccttctg ctgacctgca
     5701 tggattgctc cttgtttcga cattgtcacg tcccatttac attcgcttgc tgcgtgtgca
     5761 gggagtggga gttatttttc tttgcggaag gggccgcaaa ggtccagaat tggctcaatt
     5821 tccctccatc caggaagggt ttgtaacaga ataccataat gttggtgtgt gtgttcttat
     5881 ctggttaaga gaaagtgaaa aaaacacagc aaaaagaaat cgaaaatgtg acaaatatca
     5941 caggtgttcg tcaaattgag ttatggcggc gtgatgattt acaacaccca cggttagatg
     6001 aggtcgcgga agaagttccc gttgcgctgg tctacaacgg catttcgcat gtggtgatga
     6061 tggcgtcgcc caaagacctt gagtactttg cgctcggttt ttcgctttcc gaggggataa
     6121 tagaaagtcc gcgcgatatc ttcggcatgg atgtcgttcc ttcctgtaat ggtcttgaag
     6181 tacaaattga gctttccagc cgccgcttta tggggttgaa ggagcgccgc cgggcgctgg
     6241 cgggacgtac gggctgtggc gtatgcggcg tggagcaact taatgacatc ggaaaaccgg
     6301 tgcagccgct accgttcacc cagacgtttg atctcaacaa actggatgat gcattacgtc
     6361 atctcaacga ttttcagcca gtggggcaac tgactggttg tactcacgcc gctgcctgga
     6421 tgttgccatc tggcgaactg gtcggcgggc atgaagacgt gggtcgccat gtggcgctgg
     6481 acaaactgtt aggccgccgg tcacaagaag gggaaagctg gcagcaaggt gcggtactgg
     6541 tttccagtcg tgccagttat gaaatggtgc aaaagtcggc gatgtgcggc gtagagattt
     6601 tgtttgcggt gtctgccgcg accacgcttg ctgtagaagt ggctgagcgt tgtaatctga
     6661 cgctggtggg tttttgtaag ccgggtcggg caacggttta tacccatccg cagcgtttga
     6721 gcaattaatt aaa
//
LOCUS       DAZKHF020000236         6715 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2201S31241
            SAMN43267800-rid25106413.denovo.238, whole genome shotgun sequence.
ACCESSION   DAZKHF020000236 DAZKHF020000000
VERSION     DAZKHF020000236.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267800
            Sequence Read Archive: SRR30307263
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6715)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6715)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 6715)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350581.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350581.2
            Genome Coverage       :: 151x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:21:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,652
            CDSs (total)                      :: 5,557
            Genes (coding)                    :: 5,320
            CDSs (with protein)               :: 5,320
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 237
            CDSs (without protein)            :: 237
            Pseudo Genes (ambiguous residues) :: 0 of 237
            Pseudo Genes (frameshifted)       :: 102 of 237
            Pseudo Genes (incomplete)         :: 130 of 237
            Pseudo Genes (internal stop)      :: 52 of 237
            Pseudo Genes (multiple problems)  :: 41 of 237
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6715
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267800-rid25106413.denovo.238"
                     /serotype="O 26:H11"
                     /isolate="2201S31241"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-01"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            <1..278
                     /locus_tag="ACHOSC_RS19505"
                     /old_locus_tag="pgaptmp_003901"
     CDS             <1..278
                     /locus_tag="ACHOSC_RS19505"
                     /old_locus_tag="pgaptmp_003901"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001219937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DNA (cytosine-5-)-methyltransferase"
                     /protein_id="HGU4549744.1"
                     /translation="KKQVKGIYDKEGFRAWLLNEKKLTKRTSSDIISRCCRGVSFFDS
                     EGVDFYNCEIDEIIMKLERLESFVRLGVSLKSQLRRAFKLYYEYCRR"
     gene            complement(506..664)
                     /locus_tag="ACHOSC_RS19510"
                     /old_locus_tag="pgaptmp_003902"
     CDS             complement(506..664)
                     /locus_tag="ACHOSC_RS19510"
                     /old_locus_tag="pgaptmp_003902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005761324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="HGU4549745.1"
                     /translation="MHEPPVQPDRCALSGNYRLESNPERHDLTPLAAATGNWTRWIEI
                     LRVLEVVA"
     gene            complement(1324..1515)
                     /locus_tag="ACHOSC_RS19515"
                     /old_locus_tag="pgaptmp_003903"
     CDS             complement(1324..1515)
                     /locus_tag="ACHOSC_RS19515"
                     /old_locus_tag="pgaptmp_003903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000165985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="HGU4549746.1"
                     /translation="MTKQEKTALNMARFIRSQTLTLLEKLNELDADEQADICESLHDH
                     ADELYRSCLARFGDDGEDL"
     gene            1580..1903
                     /locus_tag="ACHOSC_RS19520"
                     /old_locus_tag="pgaptmp_003904"
     CDS             1580..1903
                     /locus_tag="ACHOSC_RS19520"
                     /old_locus_tag="pgaptmp_003904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000955993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
                     /protein_id="HGU4549747.1"
                     /translation="MLTIRVTDDEHARLLERCEGKQLAVWMRRVCLGEPVARSGKLPT
                     LAPPLLRQLAAIGNNLNQTARKVNSGQWSSGDRVQVVAALMAIEGELRSLRQVVREQG
                     ARDDC"
     gene            2112..2543
                     /locus_tag="ACHOSC_RS19525"
                     /old_locus_tag="pgaptmp_003905"
                     /pseudo
     CDS             2112..2543
                     /locus_tag="ACHOSC_RS19525"
                     /old_locus_tag="pgaptmp_003905"
                     /inference="COORDINATES: protein motif:HMM:NF015397.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
     gene            2581..3099
                     /locus_tag="ACHOSC_RS19530"
                     /old_locus_tag="pgaptmp_003906"
     CDS             2581..3099
                     /locus_tag="ACHOSC_RS19530"
                     /old_locus_tag="pgaptmp_003906"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001447511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeB family mobilization protein"
                     /protein_id="HGU4549748.1"
                     /translation="MSNLLQTGAEFEKKLKERAESTEKMLNDEFRKLGESVSEAVTSN
                     ETKIRDAIALFTASTEKSLEKHREGVKEAMMQHRKDVLKLAGNTGMMLLGIVFLLFTA
                     SGGTLWYLGGRIQANLEEIRKQEETLQKLNAKTWGVEFVQDGRRKFLVIPQGMSAEVT
                     HYQGKDWIQLTE"
     gene            3106..3339
                     /locus_tag="ACHOSC_RS19535"
                     /old_locus_tag="pgaptmp_003907"
     CDS             3106..3339
                     /locus_tag="ACHOSC_RS19535"
                     /old_locus_tag="pgaptmp_003907"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000137438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeD family mobilization/exclusion protein"
                     /protein_id="HGU4549749.1"
                     /translation="MTELETHLLNALEQLQQDYMQRLNEWESAFVELQKMFSLTQRDN
                     AMLNERVMQLSQQVQHLSERTERLSQLYSENWR"
     gene            complement(3714..3920)
                     /locus_tag="ACHOSC_RS19540"
                     /old_locus_tag="pgaptmp_003908"
     CDS             complement(3714..3920)
                     /locus_tag="ACHOSC_RS19540"
                     /old_locus_tag="pgaptmp_003908"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001096539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4549750.1"
                     /translation="MAKLPQEKPKEVIQAGFDDVKKAYKKGAKTVNDTYAEQAGRDLQ
                     SGDGKLMATYRRNKKAIDKLDAEG"
     gene            complement(3913..4296)
                     /locus_tag="ACHOSC_RS19545"
                     /old_locus_tag="pgaptmp_003909"
     CDS             complement(3913..4296)
                     /locus_tag="ACHOSC_RS19545"
                     /old_locus_tag="pgaptmp_003909"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000065853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4549751.1"
                     /translation="MSNIVHLSDKKQKNKPVDISFDDYTDNLSKGFVKNLSNKQKKEL
                     ISKIKAILKSRNVAFRSTPPSDWKEKSRTFAIFFPEKKIVFTLGVVNKLKIEDGWINH
                     KISDTEDAVLSVGKIINLIEDSQHG"
     gene            complement(4687..5748)
                     /locus_tag="ACHOSC_RS19550"
                     /old_locus_tag="pgaptmp_003910"
     CDS             complement(4687..5748)
                     /locus_tag="ACHOSC_RS19550"
                     /old_locus_tag="pgaptmp_003910"
                     /EC_number="3.1.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000034299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0009036 - type II site-specific
                     deoxyribonuclease activity [Evidence IEA];
                     GO_process: GO:0009307 - DNA restriction-modification
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HindVP family restriction endonuclease"
                     /protein_id="HGU4549752.1"
                     /translation="MSKIESLYGLKNTNRKESDLWGKNQFNSSFPTSLACYMRDNKMN
                     PIYLYLDKDLKVHAKEVSFDFVFNTTLKNSDLEFQFEKKYEPYQQYAYDDIKGIDLVI
                     KGNNSFLKALEIKLTVLPDSTTYKENESEWSSEIVIRPASTSYCALGIMHSCSDKFHE
                     IRKIFESTCESIQHWGNETEVLSKKENILNCLNTFQENFLDYQKPFLMQPLWKTKGKS
                     PILDQNAFDIFIWSDFALCRTFIDRSSYNNSSASRLMRSSARLARMVYEIASKNKTNI
                     NRIYTEMNFGYQSDKEFSLNGKITKAYMDHPRRTKPILPPSVLKEIILNGGQNKLSPE
                     RRFDQSVYYTASHLFDEIK"
     gene            5836..>6715
                     /locus_tag="ACHOSC_RS19555"
                     /old_locus_tag="pgaptmp_003911"
     CDS             5836..>6715
                     /locus_tag="ACHOSC_RS19555"
                     /old_locus_tag="pgaptmp_003911"
                     /EC_number="2.1.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001219937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003886 - DNA
                     (cytosine-5-)-methyltransferase activity [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA cytosine methyltransferase"
                     /protein_id="HGU4549753.1"
                     /translation="MRIIDLFSGCGGLSLGFLKGGFDVVGAYDFWDPAIECYRDNFSH
                     PIKKLDLSNVDDVVRELKDIDFDMIIGGPPCQDFSHAGLRIEGARANLTRSFSEIIKR
                     IKPKWFVMENVDRALRSGAYLEARGIFKESGYGLTEIVLDASKCGVPQKRKRLFVIGK
                     LDVRDGFILNEVMCGISKDSMTVRNYLGDSLGIEYYYRHPRNYNRRAIFSIDEPAPTV
                     RGVNRPIPDGYLGHAGDPVSISENVRPLTTFERARLQTFPEDFKFKGAKTNLEQMIGN
                     AVPVELAKYVAVTIMEY"
BASE COUNT         1768 a         1310 c         1751 g         1886 t
ORIGIN      
        1 aaaaaaaaca agtaaaaggt atttatgata aagaaggctt tagggcgtgg ttgctgaatg
       61 aaaagaaact aacaaaaaga acctctagcg atattatatc tagatgttgc agaggtgttt
      121 cattttttga ttcagagggg gtggattttt ataactgtga aatagatgaa attattatga
      181 aactggaaag gcttgaatcg tttgtcaggc ttggggttag cctgaagtct cagttaagaa
      241 gggcttttaa attatattac gaatattgtc gaaggtaata aatacgaatt tatgtctctc
      301 aggggagggg gaggggataa aaagatcaaa ggatcctctt gtgatccttt ttttctgcgc
      361 gtaatctgct gcctgtaaac aaaaaaacca ccgctaccaa cggtggtttg tttgccggaa
      421 tctagagcta ccaactcttt cttccgaagg taactggctt ggtggagcgc agataccaaa
      481 tactgtcctt ctagtgtagc cgcagttagg ccaccacttc aagaactctt aatatctcaa
      541 tccaccttgt ccagttacca gtggctgctg ccagtggcgt taagtcgtgt ctttccgggt
      601 tggactcaag acgatagtta ccggataagg cgcagcggtc gggctgaacg gggggttcgt
      661 gcacacagcc cagcttggag cgaacgacct acaccgaact gagataccaa cagcgtgagc
      721 tatgagaaag cgccacgctt cccgaaggga gaaaggcgga caggtatccg gtaagcggca
      781 gggtcggaac aggagagcgc acgagggagc ttccaggggg aaacgcctgg tatctttata
      841 gtcctgtcgg gtttcgccac ctctgacttg agcgtcgatt tctgtgatgc tcgtcagggg
      901 ggcggagcct atggaaaaac gccagcggcg cggccttttg ttgtcctttg cctttttgct
      961 cacatgttct ttccggcctt atcccctgat tctgtggata accgtattac cgcctttgag
     1021 tgagccgact ccgctcgccg cagccgaacg accgagcgta gcgagtcagt gagcgaggaa
     1081 gcggaagagc gcctgatgcg gtattttctc cttacgcatc tgtgcggtat ttcacaccgc
     1141 atatggtgct cttttcttat aatccgcact gatgccacat agttaagcca gtatacactc
     1201 cgctatcgct acgtgactgg ttcagggctt cgccccgaaa ccccctgacg cgccctgacg
     1261 ggcttgtctg ctcccggcat ccgctcacag gcaagctgtg accgtctccg ggagctgcat
     1321 gtgtcagagg tcttcaccgt catcaccgaa acgcgcgagg cagctgcggt aaagctcatc
     1381 ggcgtggtcg tgaagagatt cacaaatatc ggcctgttca tctgcgtcca gttcgttgag
     1441 tttctccagc agcgttaatg tctggcttct gataaagcgg gccatgttaa gggcggtttt
     1501 ttcctgtttg gtcactgatg cctccgtgta agggggattt ctgttcatgg gggtaatgat
     1561 accgatgaaa cgcgagagga tgctcacgat acgggttact gatgatgaac atgcccggtt
     1621 actggaacgc tgtgagggta aacaactggc ggtatggatg cggcgggtct gcctgggtga
     1681 gccggttgcc cgttccggga agctgccgac actggcaccg ccgttactgc gtcagctggc
     1741 cgccatcggg aataacctga atcagacagc gaggaaggta aacagcgggc agtggtcttc
     1801 cggtgaccgg gttcaggtgg tggccgcact gatggccatc gagggagagt tgcgcagctt
     1861 acggcaggtc gtcagggagc agggagcgcg ggatgattgt taaatttcat gccaggggaa
     1921 aaggcggtgg cagtggtccg gttgattacc tgctgggaag ggagcgtaac cgggaagggg
     1981 caacggtgct tcagggtaat ccggaagaag tcagggaact catcgatgcc acgccatttg
     2041 cgaagaaata cacgtccggt gttctgtcgt tcgcggagaa ggaactgccg ccgggaggac
     2101 gtgaaaaagt gatggagagc tttgagcgcg tactgatgcc cggtctcgaa aaaaatcagt
     2161 acagcattct gtgggtggag caccaggaca aggggcgtct tgagctgaat tttgtcattc
     2221 cgaacatgga gctacagacc ggaaaacgcc tccagccgta ctacgaccga gcagacaggc
     2281 cgagaattga tgcctggcag acactggtga atcaccatta cgggctgcat gaccccaacg
     2341 ccccggagaa ccgccggata ctgactctct ctgataacct gccggaaacg aaacaggcgc
     2401 ttgctgagag cgtcacacgc gggatagacg ctctttatca tgtaggggag ataaaaggcc
     2461 gtcaggatgt gattcaggcg ctcacagagg cggggcttga agtggtcagg gtgacgcgaa
     2521 gcagcatcag cattgcagac ccgaacggcg ggaagaatat caggctgaaa ggagcgtttt
     2581 atgagcaatc ttttacagac gggcgcggag ttcgagaaaa agctgaaaga gagagccgaa
     2641 tctacagaga aaatgctgaa cgacgagttc aggaagctcg gcgagtctgt cagcgaggct
     2701 gtgacctcaa acgagacgaa aatcagagac gctatagccc tgttcacagc ctcgacagag
     2761 aaatcgctgg aaaaacacag ggaaggggtg aaagaggcga tgatgcagca caggaaggac
     2821 gtgttaaagc tggcagggaa tacgggcatg atgttactgg ggatagtctt tctcctgttt
     2881 accgcgagtg gcgggacgct ctggtatctt ggcgggagga tacaggcgaa cctggaagaa
     2941 atcaggaagc aggaagagac attgcagaaa ctgaacgcga agacatgggg cgtggagttt
     3001 gtgcaggacg ggagaaggaa atttctggtc attccgcaag ggatgtcagc agaggtgacg
     3061 cactatcagg ggaaggactg gatacaactg acggagtgat acggaatgac agagctggaa
     3121 acacatttgc tgaacgcctt agagcagctg caacaggact atatgcagcg gctgaacgaa
     3181 tgggagagcg ccttcgtgga attgcagaag atgttttcgc ttacgcaacg ggacaacgcg
     3241 atgctgaacg agcgggtcat gcagttgagt cagcaggtgc agcacttgag cgagcggaca
     3301 gaacgcttga gccagttata cagcgagaac tggagataag agaggaacgg ctgatacagg
     3361 agcgcgaaca tgcgttatcc ctggaacggg agcgtcagcc ggaaatacag gaacgcatac
     3421 tggatggtcc ttcgttgtcg ctgtgatggc gaaactataa aaaatggcag gttcagtgga
     3481 ttttgacgga ctaatgtagt ctgcaccatc tggttgcata ggtattcatg cggttaaaat
     3541 ttatcaggcg cgatcgcggc agtttttcgg gtggtttgtt gccattttta cctgtctgct
     3601 gccgtgatcg caccgaacgc gttttagcgg tgtgcgtaac ggatgttatg gtaaatacaa
     3661 tatgcagcca gtcttagaga cgaaaagtcc gcccagaaga ctgacttttc agatcaacct
     3721 tcagcatcta acttgtctat cgctttttta ttgcgccgat aggtagccat tagtttacca
     3781 tcgcctgatt gtaagtcacg acctgcttgc tcagcatagg tatcgtttac agtcttagcg
     3841 ccttttttat aggccttttt aacatcatca aatccggctt ggatgacttc tttcggcttt
     3901 tcttgaggta atttagccat gctgtgaatc ctctataagg ttgattattt ttccaacaga
     3961 taagacggca tcttctgtgt cgctgatttt atgatttatc caaccgtcct ctatctttaa
     4021 tttgttcact actcctaatg taaaaacaat ctttttttca ggaaagaaaa ttgcaaaggt
     4081 tctacttttc tctttccaat cagagggcgg cgtcgaacgg aacgctacat tcctcgattt
     4141 aagtatcgct ttgattttac taattaattc ttttttttgt ttattgctga gatttttgac
     4201 gaaccctttg ctgagattgt ctgtgtaatc atcaaaagaa atatcaactg gtttgttttt
     4261 ttgtttttta tcagatagat gaacaatgtt tgacactgga tatcctgctt tttgatgaat
     4321 gaatactcag aagagtagcg gaactgtctt gttagtcaca gaggtagagt atcaattcta
     4381 cgatagatgg gttcgaagat ccggctgaga ccgttcagta cgcggtcgag atgttatgtt
     4441 caatgtaatg accgtcgaaa ttgcctttac gggcatgaag caaccaggag caatcattgg
     4501 caaaggtaac tagagttcaa tgcatggtaa tcatttttga gcttgtgatg catagagttg
     4561 gttgctgttc cattgcgtct caaactccgt caagatgctc gccgtaaaag cgagcatgtc
     4621 tagtatgcct gttccgctac tgatttttga tgggcgaatt cagggctggc gagccaccaa
     4681 gaaaaattac tttatttcat caaacaggtg agatgcagta taataaacgc tttgatcaaa
     4741 gcgtctctct gggcttaatt tattttgccc gccatttaaa attatttctt ttaatactga
     4801 tggtgggagg atgggttttg ttcttcttgg gtgatccata taagcttttg ttatttttcc
     4861 gtttagcgag aactctttat cactttgata tccaaagttc atctcagtat aaattctatt
     4921 gatgtttgtc ttgtttttgc tcgcaatttc atacaccatt ctggcgagtc tggctgatga
     4981 tctcattagt ctgctggcag atgaattgtt atatgaggat ctatcaataa aggttcgaca
     5041 caaagcaaaa tcactccaga tgaatatgtc aaaggcattt tgatctaata ttgggctctt
     5101 gccttttgtt ttccataatg gttgcattaa aaaaggtttc tggtaatcaa gaaaattttc
     5161 ttggaaagtg tttaggcaat ttaatatatt ttctttcttt gaaagaactt cagtttcatt
     5221 cccccagtgc tgtatgcttt cgcatgtgga ttcaaatatt tttcttattt catggaattt
     5281 atcgctgcaa gagtgcatta tgccaagggc acaataagaa gtgcttgcag gtcttattac
     5341 aatctctgaa ctccattcgc tctcgttttc tttatatgtc gtgctatctg gaagtacggt
     5401 taattttatt tctagtgctt ttaaaaaact attgtttcct tttataacta gatctattcc
     5461 ttttatgtca tcataagcat actgttggta tggttcatat tttttctcaa attgaaattc
     5521 aagatcacta ttttttaatg ttgtgttaaa aacgaagtca aaagatactt ctttggcatg
     5581 cacttttaaa tccttatcta aataaagata aatcggattc attttgttgt ctctcatata
     5641 acatgctaga gaggtgggaa aagaactgtt aaattggttc tttccccaca gatcgctctc
     5701 tttcctgttg gtgtttttta aaccataaag agattctatt ttgctcactt tttgctcctg
     5761 acgtcaattt attgacgtct atggtgtgtt tctatatcat gatgtggagt gatatgataa
     5821 tgagtttttt atgttatgag aataattgat ctttttagtg ggtgtggggg gttgtcccta
     5881 ggctttttga agggggggtt tgatgttgta ggtgcttatg atttctggga tcctgctatt
     5941 gaatgttaca gagataattt tagccatccg ataaaaaaac tagatttgag caatgttgat
     6001 gatgtggtga gggagcttaa agatatagac ttcgatatga ttattggagg gcctccttgc
     6061 caagacttca gtcatgcagg tttaagaatt gaaggtgcta gagctaactt aactcgctca
     6121 ttttcagaaa ttataaaaag aataaagcca aagtggtttg tgatggaaaa tgtagatcgg
     6181 gcattaagaa gtggtgccta tttagaagct cgaggaatct ttaaagaatc agggtatggg
     6241 ttaacagaaa ttgttttgga tgccagtaag tgtggtgttc cgcaaaaaag aaaaagatta
     6301 tttgtaattg gaaagcttga cgtgagggat ggttttatcc ttaacgaagt gatgtgcggg
     6361 atatcgaaag atagtatgac agttagaaat tatcttggtg attcattggg gattgagtat
     6421 tattacagac atccgagaaa ttataacaga agggctattt tctctattga tgaacctgcg
     6481 ccaacagtaa gaggggttaa tagaccgata cctgatggat atttagggca tgcaggcgat
     6541 cctgtcagta tttctgagaa tgtaaggcca ttaacaacct ttgaaagagc aagattgcaa
     6601 acatttcctg aagactttaa atttaaaggt gcaaaaacaa atttggagca aatgataggt
     6661 aatgctgttc cggttgagtt agcaaaatat gttgcggtga caatcatgga gtatg
//
LOCUS       DAZKHF020000237         6675 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2201S31241
            SAMN43267800-rid25106413.denovo.239, whole genome shotgun sequence.
ACCESSION   DAZKHF020000237 DAZKHF020000000
VERSION     DAZKHF020000237.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267800
            Sequence Read Archive: SRR30307263
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6675)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6675)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 6675)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350581.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350581.2
            Genome Coverage       :: 151x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:21:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,652
            CDSs (total)                      :: 5,557
            Genes (coding)                    :: 5,320
            CDSs (with protein)               :: 5,320
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 237
            CDSs (without protein)            :: 237
            Pseudo Genes (ambiguous residues) :: 0 of 237
            Pseudo Genes (frameshifted)       :: 102 of 237
            Pseudo Genes (incomplete)         :: 130 of 237
            Pseudo Genes (internal stop)      :: 52 of 237
            Pseudo Genes (multiple problems)  :: 41 of 237
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6675
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267800-rid25106413.denovo.239"
                     /serotype="O 26:H11"
                     /isolate="2201S31241"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-01"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            complement(209..454)
                     /locus_tag="ACHOSC_RS19560"
                     /old_locus_tag="pgaptmp_003912"
     CDS             complement(209..454)
                     /locus_tag="ACHOSC_RS19560"
                     /old_locus_tag="pgaptmp_003912"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000820629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4549754.1"
                     /translation="MKRHRLTEAEERRLAEAIAAEARAMGLTKKDKMPQETKRRLRAR
                     ILKAILDERTASKNREATLTAGQDPDDTFTWQPLRRR"
     gene            718..2811
                     /gene="cda"
                     /locus_tag="ACHOSC_RS19565"
                     /old_locus_tag="pgaptmp_003913"
     CDS             718..2811
                     /gene="cda"
                     /locus_tag="ACHOSC_RS19565"
                     /old_locus_tag="pgaptmp_003913"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016245160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin-D"
                     /protein_id="HGU4549755.1"
                     /translation="MSDYEGSGPTEGIDYGHSMVVWPSTGLISGGDVKPGGSSGIAPS
                     MPPGWGDYSPQGIALVQSVLFPGIIRRIILDKELEEGDWSGWSVSVHSPWGNEKVSAA
                     RTVLENGLRGGLPEPSRPAAVSFARLEPASGNEQKIIRLMVTQQLEQVTDIPASQLPA
                     AGNNVPVKYRLMDLMQNGTQYMAIIGGIPMTVPVVDAVPVPDRSRPGTNIKDVYSAPV
                     SPNLPDLVLSVGQMNTPVLSNPEIQEEGVIAETGNYVEAGYTMSSNNHDVIVRFPDGS
                     GVSPLYISTVEILDSNGLSQRQEAENKAKDDFRVKKEEAVARAEAEKAKAELFSKAGV
                     NQPPVYTQEMMERANSVMNEQGALVLNNTASSVQLAMTGTGVWTAAGDIAGNISKFFS
                     NALEKVTIPEVSPLLMRISLGALWFHSEEAGAGSDIVPGRNLEAMFSLSAQMLAGQGV
                     VIEPGATSVNLPVRGQLINSNGQLALDLLKTGNESIPAAVPVLNAVRDTATGLDKITL
                     PAVVGAPSRTILVNPVPQPSVPTDTGNHQPVPVTPVHTGTEVKPVEMPVTTITPVSDV
                     GGLRDFIYWRPDAAGTGVEAVYVMLNDPLDSGRFSRKQLDKKYKHAGDFGISDTKKNR
                     ETLTKFRDAIEEHLSDKDTVEKGTYRREKGSKVYFNPNTMNVVIIKSNGEFLSGWKIN
                     PDADNGRIYLETGEL"
     gene            2808..3071
                     /locus_tag="ACHOSC_RS19570"
                     /old_locus_tag="pgaptmp_003914"
     CDS             2808..3071
                     /locus_tag="ACHOSC_RS19570"
                     /old_locus_tag="pgaptmp_003914"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032488334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015643 - toxic substance binding
                     [Evidence IEA];
                     GO_process: GO:0030153 - bacteriocin immunity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="colicin immunity domain-containing protein"
                     /protein_id="HGU4549756.1"
                     /translation="MNKMAMIDLAKLFLASKITAIEFSERICVERRRLYGVKDLSPNI
                     LNCGEELFMAAERFEPDADRADYEIDDNGLKVEVRSILEKFKL"
     gene            3141..3287
                     /locus_tag="ACHOSC_RS19575"
                     /old_locus_tag="pgaptmp_003915"
     CDS             3141..3287
                     /locus_tag="ACHOSC_RS19575"
                     /old_locus_tag="pgaptmp_003915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000711480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA];
                     GO_process: GO:0019835 - cytolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="colicin release lysis protein"
                     /protein_id="HGU4549757.1"
                     /translation="MKKKIGGSMILVLAVLCLTACQANYVRDVQGGTIAPSSSSKLIG
                     VAVQ"
     gene            3994..4182
                     /locus_tag="ACHOSC_RS19580"
                     /old_locus_tag="pgaptmp_003916"
     CDS             3994..4182
                     /locus_tag="ACHOSC_RS19580"
                     /old_locus_tag="pgaptmp_003916"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001479923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4549758.1"
                     /translation="MDNTITAFEWADTARRSRTTERSESVSEEAEKRLDEHFLLTHLC
                     GISHPAWCTFHTILTDAT"
     gene            complement(4334..4525)
                     /locus_tag="ACHOSC_RS19585"
                     /old_locus_tag="pgaptmp_003917"
     CDS             complement(4334..4525)
                     /locus_tag="ACHOSC_RS19585"
                     /old_locus_tag="pgaptmp_003917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000165985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="HGU4549759.1"
                     /translation="MTKQEKTALNMARFIRSQTLTLLEKLNELDADDQADICEALHDH
                     ADELYRSCLARFGDDGENL"
     gene            4593..4916
                     /locus_tag="ACHOSC_RS19590"
                     /old_locus_tag="pgaptmp_003918"
     CDS             4593..4916
                     /locus_tag="ACHOSC_RS19590"
                     /old_locus_tag="pgaptmp_003918"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000956000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
                     /protein_id="HGU4549760.1"
                     /translation="MLTIRVTDEEHARLLARCEGKQLASWMRKVCLGAPPSKTSGLPT
                     LAPPLLRQFASVGNNLNQIARKINSGQWSGHDRVHVVAALMAIGRELSELRDEVRKQG
                     ERDDS"
     gene            5125..5559
                     /locus_tag="ACHOSC_RS19595"
                     /old_locus_tag="pgaptmp_003919"
                     /pseudo
     CDS             5125..5559
                     /locus_tag="ACHOSC_RS19595"
                     /old_locus_tag="pgaptmp_003919"
                     /inference="COORDINATES: protein motif:HMM:NF015397.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
     gene            5594..6154
                     /locus_tag="ACHOSC_RS19600"
                     /old_locus_tag="pgaptmp_003920"
     CDS             5594..6154
                     /locus_tag="ACHOSC_RS19600"
                     /old_locus_tag="pgaptmp_003920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000067489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeB family mobilization protein"
                     /protein_id="HGU4549761.1"
                     /translation="MSNLLQMGTDFEKKLKERAVSTENMLNSEFRKLEASVDRELNLN
                     GQKIRDAISAHTAAVKEQLETLNSAVNAQFSATENELARRQEELLWKLVKGRILYPSL
                     TALCVTVGIFLASWGLIQWQESRIAANILAIRDQEETLAKLREKTWGVTYHEGRNGKF
                     LVLPSGVKGENNWTVEKKNAVRLVRE"
     gene            6161..6394
                     /locus_tag="ACHOSC_RS19605"
                     /old_locus_tag="pgaptmp_003921"
     CDS             6161..6394
                     /locus_tag="ACHOSC_RS19605"
                     /old_locus_tag="pgaptmp_003921"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000137429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeD family mobilization/exclusion protein"
                     /protein_id="HGU4549762.1"
                     /translation="MTELEMHLLNALEQLQQDYTQRLKDWESAFTDLQHMFELTRQEN
                     GQLREQCDDLSQQVLRLAGQVDRLSRLFITNSR"
BASE COUNT         1661 a         1422 c         1936 g         1656 t
ORIGIN      
        1 aaaaaaaaca gtgtcatgag gtttacgcag gcaaaaccag cgttattcac atggctgaat
       61 agctatgcat acggttaaaa tttatcaggt gcgatcgcgg caacttttcg ggtggtttgt
      121 tgccattttt acctgtcagc agccgtgatc gcgctgaacg catttcagcg gtgcgtacaa
      181 taaggggtta tgttaaataa cccttagctt agcggcggcg cagtggttgc caggtgaagg
      241 tgtcatccgg gtcctgtccg gcagtcagtg tggcttctcg atttttgctg gccgttcgtt
      301 catcaagaat ggctttaagt atccgtgccc ggaggcgtcg ctttgtttcc tgcggcattt
      361 tgtccttttt cgtcagcccc atggcacgag cttcagcggc aatggcttct gccagtcggc
      421 gctcttcggc ttcggtcagt ctatggcgtt tcatggtggt ctccgggtgt ttctctggtg
      481 tcataacagt ttaactgttg gctctctcct tatgctaatc agccttcttt catgcctgtt
      541 gccattgagt ttaagaatga ttggttgttt ttcttgagcg ttttcacaaa aacgtaaatc
      601 cagtgggttg acaggattta cgaggtgacg taacttttta ctgtacataa aaccagtggt
      661 tttatgtaca gtttgttgtt tttaacgcat tgttttaaaa gtcaaagagg tgtttttatg
      721 agtgattacg aaggtagtgg tccgacagaa ggtattgatt atgggcactc gatggtcgtg
      781 tggccgtcaa caggactgat ttcgggtggt gatgtgaaac caggaggctc atcaggtatc
      841 gctccatcca tgcctccggg atggggggat tacagcccac agggtatcgc acttgtacaa
      901 agtgttcttt ttccgggaat tattcgccgg attattctgg ataaggaact tgaagaggga
      961 gactggtcgg gatggtctgt cagtgtgcat agcccttggg gaaacgagaa agtttccgct
     1021 gcacgaacag ttcttgagaa tggtttacgt ggtggtttgc cagaaccgtc tcgcccggct
     1081 gctgtttctt ttgcccgtct ggagcctgct tccggaaatg agcaaaaaat tattcgtctt
     1141 atggttacac agcaactgga acaggtaacg gatattccgg ccagccagtt accagcagcg
     1201 ggtaataatg taccggtgaa atatcgtctg atggacctta tgcagaacgg tacgcaatat
     1261 atggctatta tcggaggtat tccgatgaca gtgccggtag ttgatgccgt tccagttccg
     1321 gaccggagtc gtccgggaac aaatattaaa gatgtttaca gtgcccctgt atcaccaaat
     1381 ctaccggacc tggtattaag tgtgggtcag atgaatactc cggttctgtc taatcccgaa
     1441 attcaggaag aaggagttat tgctgagaca ggtaattatg ttgaggctgg ttatacgatg
     1501 tccagtaata atcatgatgt cattgttcgt tttcctgatg gcagtggtgt ttctccgtta
     1561 tatatttcaa ctgtagagat tctggacagt aatggtctga gtcagcgtca ggaagccgaa
     1621 aataaagcaa aggatgattt cagagtcaag aaagaagaag cggtagcccg ggctgaagct
     1681 gaaaaagcaa aagccgagtt atttagtaaa gcaggtgtga accagcctcc tgtatataca
     1741 caggaaatga tggaaagggc taattcagta atgaatgagc agggagctct tgttctgaat
     1801 aacactgcca gttctgtaca actggcgatg accggaactg gtgtctggac tgctgctggt
     1861 gatattgcag gcaacataag caagtttttc agtaatgctc tggagaaagt aaccataccc
     1921 gaagtgagtc ccctgcttat gcggatttct cttggtgctc tgtggtttca ttcagaagaa
     1981 gctggagcag gaagtgatat cgtgccgggg cgaaatctgg aggcgatgtt ttcgctgagt
     2041 gctcagatgt tggctggaca gggcgtggtc attgaacctg gtgcgacgag tgtaaatctg
     2101 cctgttcgtg gacaattgat aaacagtaac gggcaattag ctctggattt actgaaaaca
     2161 gggaatgaaa gtatcccagc tgcggttcct gttcttaatg ctgtccgtga tacagcaaca
     2221 ggactggata aaatcacgtt accagcagta gtaggcgcac cttcccggac gattctggtt
     2281 aatccggtac cacaaccttc ggtgccaaca gatacaggta atcatcaacc cgttccggtt
     2341 acaccagtgc atacaggaac ggaagtaaaa ccggtcgaaa tgccagtaac gacgattact
     2401 cctgtttctg atgttggtgg actacgagat tttatttact ggcgtcctga tgctgctggg
     2461 actggtgttg aagctgttta tgtgatgctt aatgatcctc tggattcagg gcgattttcg
     2521 cgtaaacaac ttgataaaaa atataaacat gctggtgatt ttggtattag tgatacaaaa
     2581 aagaatcgtg aaactcttac taaattcagg gatgctattg aggagcattt atcggataag
     2641 gatacagtag agaaaggaac ataccgaaga gaaaaaggtt caaaagttta ttttaatcct
     2701 aatacgatga atgtggttat aattaagtca aatggtgagt tcttatctgg gtggaaaata
     2761 aatccagatg cggataatgg tcgaatttat ttagagacgg gtgaactatg aacaagatgg
     2821 caatgatcga tttggcgaaa ttatttttag cttcgaaaat tacagcaatt gagttttcag
     2881 agcgaatttg tgttgaacgg agaagattgt atggtgttaa ggatttgtct ccgaatatat
     2941 taaattgtgg ggaagagttg tttatggctg ctgagcgatt tgagcctgat gcagataggg
     3001 ctgattatga aattgatgat aatggactta aggttgaggt ccgatctatc ttggaaaaat
     3061 ttaaattata atcatgagtg attttaatat ctggcattta agtataaatg tcagatattc
     3121 actcatgaca aggagatgtc atgaaaaaaa agataggggg atctatgatt ctcgttcttg
     3181 ctgttttgtg tctgactgct tgccaagcta actatgtaag ggatgttcag ggtggaacaa
     3241 ttgctccatc atcatcttcg aaactaatcg gggtggcggt tcagtgaaaa atattaaagg
     3301 atcttcttga gatccttttt ttctgcgcgt aatctgctgc ctgtaaacga aaaaacccgc
     3361 cttgtggcgg gtccttccgt ccgatcagaa gtcttcgacc tcttctaatc ggcggtggta
     3421 actggcttgg aggagcacag tcaccaaaac tgtcttttca gtttagcctt aactggttca
     3481 taacttcaag agcaactcct ctaaatcagt taccaatggc tgctgccagt ggcgttaagt
     3541 cgtgtctttt ccgggttggc cccaagagaa cagttaccgg agaaggtgca gcagtcgggc
     3601 tgaacggggg gttcctgcat acagcccagc ccggagcgaa ctgcctaagt cgccccgaga
     3661 taccagaaga cgtgagctat gagaaagcgc cacgtgtccc gcaagggaga aaggcggaaa
     3721 aggtatccgg taatcgatat ggcgggaacg tgggggcgca ggagggagcg attcaccgga
     3781 aacggtgatg atctttaagc cacgtcgggt ttcgccaccg ctggcggatt tcatggttga
     3841 gcctcacggc tcccacagat gcaccgaaaa agtgtctgtt ttgtgaagtc cgtcaggggg
     3901 gcggagccta tggaaaaagc gcctccggcg cggccttttt cggggttttc gtttcttgct
     3961 cacatgttct ttccggcgtt atcccctgat tctgtggata acactatcac cgcttttgag
     4021 tgggcagata ccgctcgccg cagccgaacg accgagcgta gcgagtcagt gagcgaggaa
     4081 gcggaaaagc gcctggacga gcattttctc cttacgcatc tgtgcggcat ttcacacccg
     4141 gcatggtgca cttttcatac catcctcact gatgccacat gattaagcca gtatacactc
     4201 cgctatcgct acgtgactgg gtcatggctg cgccccgaca cccgctaaaa cccgctgacg
     4261 cgccctgacg ggcttgtctg ctcccggcat ccgctcacag gcaagctgtg accgtctccg
     4321 ggagctgcat gtgtcagagg ttttcaccgt catccccgaa gcgtgcgagg cagctgcggt
     4381 aaagctcgtc agcgtggtcg tgaagcgctt cgcagatgtc agcctggtcg tcggcgtcca
     4441 gttcattcag tttttccagc agggtcagcg tctggcttct gatgaagcgg gccatgttaa
     4501 gggcggtttt ttcctgttta gtcacagttc tgtttctcca tgtgtgggga gtctgtcgga
     4561 ggtttttaat gataccggtg aaacgcgaaa ggatgctcac gatacgggtc acggatgagg
     4621 aacatgcgcg gctgctggca cgttgtgagg gaaaacaact tgcgtcatgg atgcgtaagg
     4681 tctgcctggg ggcaccgcca tcgaaaacgt caggtcttcc gacactggca ccgccgctgt
     4741 tgcgccagtt cgcttcggtc ggcaataacc tgaatcagat agcccggaag ataaacagtg
     4801 gacagtggtc ggggcatgac cgggttcatg tggtggctgc actgatggcc atcggacgtg
     4861 aactgagtga gctgagggat gaggtccgta aacagggaga acgcgatgat agttaagttt
     4921 catgccaggg gaaaaggcgg tggcagtggt ccggttgatt acctgctggg ccgggagcgt
     4981 aaccgggaag gggcgagagt tctgcggggt gctcccgaag aggtgcggga actcatcgat
     5041 gccacgccgt ttgcgaaaaa atacacctcg ggggttctgt cttttgcaga gcagacactg
     5101 ccaccgggag agcgtgaaag ggtgatggag agctttgagc gggtactgat gcccggtctt
     5161 gaaaaaaatc agtacagcat cctgtgggtg gagcaccagg acaaggggcg tcttgagctg
     5221 aattttgtca tcccgaatat ggagctggcg agcggaaaac ggctccagcc ctattacgac
     5281 cgggcggacc gtccgcgaat caatgcctgg cagacactgg tgaatcacca ttacgggctg
     5341 cacgacccga acgcaccgga gaacaggcgg acacttgtca ccccgaataa tctgccgaaa
     5401 gcgaaacagg aggccgcaga agcgattacg aggggactgg aggctcttta ccatgccgga
     5461 gcgctaaaaa cgcgtcagga tgtcactgag gcgcttacag cggcaggttt tgaggtggtg
     5521 aggacaacca ggagcagtat cagtatcgcc gacccggagg gcgggcgtaa tctcaggctg
     5581 aaaggggcgc tgtatgagca atcttttaca aatggggaca gacttcgaga agaaactgaa
     5641 agagcgtgcc gtctctacag agaacatgct gaacagcgag ttcaggaagc tcgaagcgtc
     5701 tgtagacagg gaactgaact taaacgggca gaaaatcaga gacgctatca gcgcacacac
     5761 agcagcggtg aaagaacaac tggagacgct gaacagtgcc gtgaacgcgc agttcagcgc
     5821 cacagagaac gaacttgccc ggcggcagga agagctgctc tggaagctgg tcaaagggcg
     5881 gatactgtac ccgtcgctga cggcgctttg cgtcacggtc gggatttttc tggcgtcatg
     5941 ggggctgatt cagtggcagg aaagccgtat tgcggcgaac atcctggcta tcagggacca
     6001 ggaagagacg ctggcgaaac tccgggaaaa aacatggggt gtgacgtatc acgagggcag
     6061 gaacgggaaa tttctggtgt tgccgtccgg ggtgaagggg gaaaacaact ggacggtgga
     6121 gaagaagaac gcagtaagac tggtcaggga gtaagtgagt atgacagagc tggaaatgca
     6181 cttactgaac gccttagagc aattgcagca ggactatacg cagcggctga aagactggga
     6241 gagcgccttc acggatttgc agcatatgtt cgagctgaca cgacaggaga acgggcaact
     6301 gagggaacag tgcgacgact tgagtcagca ggtgctgaga ttagccgggc aggtggaccg
     6361 cttgagtcgg ttatttatca cgaacagcag ataagggatg aacaggaaag gcaggcacgc
     6421 ctggaacggg agcgtgcgcg gtcctggcat gaagagcagg aacgggaagc acgggtatgg
     6481 cgtggcccgt caatggggat gtgacggtga gttgttccgg gaggttgcca gatggtaccg
     6541 gaacgcgtta aaaacgcatc cgggcgcgat tctggcgtgt cgtttttgtt gctgtgtttt
     6601 gcctctttgc cagatcgcgc tcattcctca gcctcatccc gcgatgtctg cgtatgtcat
     6661 caactgaaaa gatgg
//
LOCUS       DAZKHF020000238         6657 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2201S31241
            SAMN43267800-rid25106413.denovo.240, whole genome shotgun sequence.
ACCESSION   DAZKHF020000238 DAZKHF020000000
VERSION     DAZKHF020000238.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267800
            Sequence Read Archive: SRR30307263
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6657)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6657)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 6657)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350581.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350581.2
            Genome Coverage       :: 151x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:21:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,652
            CDSs (total)                      :: 5,557
            Genes (coding)                    :: 5,320
            CDSs (with protein)               :: 5,320
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 237
            CDSs (without protein)            :: 237
            Pseudo Genes (ambiguous residues) :: 0 of 237
            Pseudo Genes (frameshifted)       :: 102 of 237
            Pseudo Genes (incomplete)         :: 130 of 237
            Pseudo Genes (internal stop)      :: 52 of 237
            Pseudo Genes (multiple problems)  :: 41 of 237
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6657
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267800-rid25106413.denovo.240"
                     /serotype="O 26:H11"
                     /isolate="2201S31241"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-01"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            <1..226
                     /locus_tag="ACHOSC_RS19610"
                     /old_locus_tag="pgaptmp_003922"
     CDS             <1..226
                     /locus_tag="ACHOSC_RS19610"
                     /old_locus_tag="pgaptmp_003922"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="pentapeptide repeat-containing protein"
                     /protein_id="HGU4549763.1"
                     /translation="TRLYRVNFDEASVQGMGISILRGEENIPIDSDTLVTLQKFFEED
                     CTSHTGMSQTEDNINAVAMKITADIMQHAD"
     gene            complement(380..2029)
                     /locus_tag="ACHOSC_RS19615"
                     /old_locus_tag="pgaptmp_003923"
     CDS             complement(380..2029)
                     /locus_tag="ACHOSC_RS19615"
                     /old_locus_tag="pgaptmp_003923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015385 - sodium:proton antiporter
                     activity [Evidence IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA];
                     GO_process: GO:0006885 - regulation of pH [Evidence IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter"
                     /protein_id="HGU4549764.1"
                     /translation="MEIFFTILIMTLVVSLSGVVTRVMPFQIPLPLMQIAIGALLAWP
                     TFGLHVEFDPELFLVLFIPPLLFADGWKTPTREFLEHGREIFGLALALVVVTVVGIGF
                     LIYWVVPGIPLIPAFALAAVLSPTDAVALSGIVGEGRIPKKIMGILQGEALMNDASGL
                     VSLKFAVAVAMGTMIFTVGGATVEFMKVAIGGILAGFVVSWLYGRSLRFLSRWGGDEP
                     ATQIVLLFLLPFASYLIAEHIGVSGILAAVAAGMTITRSGVMRRAPLAMRLRANSTWA
                     MLEFVFNGMVFLLLGLQLPGILETSLMAAEIDPNVEIWMLFTDIILIYAALMLVRFGW
                     LWTMKKFSNRFLKKKPMEFGSWTTREILIASFAGVRGAITLAGVLSIPLLLPDGNVFP
                     ARYELVFLAAGVILFSLFVGVVMLPILLQHIEVADHSQQLKEERIARAATAEVAIVAI
                     QKMEERLAADTEENIDNQLLTEVSSRVIGNLRRRADGRNDVESSVQEENLERRFRLAA
                     LRSERAELYHLRATREISNETLQKLLHDLDLLEALLIEENQ"
     gene            complement(2181..3530)
                     /gene="ghxP"
                     /locus_tag="ACHOSC_RS19620"
                     /old_locus_tag="pgaptmp_003924"
                     /gene_synonym="yjcD"
     CDS             complement(2181..3530)
                     /gene="ghxP"
                     /locus_tag="ACHOSC_RS19620"
                     /old_locus_tag="pgaptmp_003924"
                     /gene_synonym="yjcD"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="guanine/hypoxanthine transporter GhxP"
                     /protein_id="HGU4549765.1"
                     /translation="MSTPSARTGGSLDAWFKISQRGSTVRQEVVAGLTTFLAMVYSVI
                     VVPGMLGKAGFPPAAVFVATCLVAGLGSIVMGLWANLPLAIGCAISLTAFTAFSLVLG
                     QHISVPVALGAVFLMGVLFTVISATGIRSWILRNLPHGVAHGTGIGIGLFLLLIAANG
                     VGLVIKNPLDGLPVALGDFATFPVIMSLVGLAVIIGLEKLKVPGGILLTIIGISIVGL
                     IFDPNVHFSGVFAMPSLSDENGNSLIGSLDIMGALNPVVLPSVLALVMTAVFDATGTI
                     RAVAGQANLLDKDGQIIDGGKALTTDSMSSVFSGLVGAAPAAVYIESAAGTAAGGKTG
                     LTAITVGVLFLLILFLSPLSYLVPGYATAPALMYVGLLMLSNVAKIDFADFVDAMAGL
                     VTAVFIVLTCNIVTGIMIGFATLVIGRLVSGEWRKLNIGTVVIAVALVTFYAGGWAI"
     gene            complement(4076..4540)
                     /gene="soxR"
                     /locus_tag="ACHOSC_RS19625"
                     /old_locus_tag="pgaptmp_003925"
     CDS             complement(4076..4540)
                     /gene="soxR"
                     /locus_tag="ACHOSC_RS19625"
                     /old_locus_tag="pgaptmp_003925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="redox-sensitive transcriptional activator SoxR"
                     /protein_id="HGU4549766.1"
                     /translation="MEKKLPRIKALLTPGEVAKRSGVAVSALHFYESKGLITSIRNSG
                     NQRRYKRDVLRYVAIIKIAQRIGIPLATIGEAFGVLPEGHTLSAKEWKQLSSQWREEL
                     DRRIHTLVALRDELDGCIGCGCLSRSDCPLRNPGDRLGEEGTGARLLEDEQN"
     gene            4626..4949
                     /gene="soxS"
                     /locus_tag="ACHOSC_RS19630"
                     /old_locus_tag="pgaptmp_003926"
     CDS             4626..4949
                     /gene="soxS"
                     /locus_tag="ACHOSC_RS19630"
                     /old_locus_tag="pgaptmp_003926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide response transcriptional regulator
                     SoxS"
                     /protein_id="HGU4549767.1"
                     /translation="MSHQKIIQDLIAWIDEHIDQPLNIDVVAKKSGYSKWYLQRMFRT
                     VTHQTLGDYIRQRRLLLAAVELRTTERPIFDIAMDLGYVSQQTFSRVFRRQFDRTPSD
                     YRHRL"
     gene            complement(4952..6538)
                     /gene="pdeC"
                     /locus_tag="ACHOSC_RS19635"
                     /old_locus_tag="pgaptmp_003927"
     CDS             complement(4952..6538)
                     /gene="pdeC"
                     /locus_tag="ACHOSC_RS19635"
                     /old_locus_tag="pgaptmp_003927"
                     /EC_number="3.1.4.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="c-di-GMP phosphodiesterase PdeC"
                     /protein_id="HGU4549768.1"
                     /translation="MSHRARHQLLALPGIIFLVLFPIILSLWIAFLWAKSEVNNQLRT
                     FAQLALDKSELVIRQADLVSDAAERYQGQVCTPAHQKRMLNIIRGYLYINELIYARDN
                     HFLCSSLIAPVNGYTIAPADYKREPNVSIYYYRDTPFFSGYKMTYMQRGNYVAVINPL
                     FWSEVMSDDPTLQWGVYDTVTKTFFSLSKEASAATFSPLIHLKDLTVQRNGYLYATVY
                     STKRPIAAIVATSYQRLITHFYNHLIFALPAGILGSLVLLLLWLRIRQNYLSPKRKLQ
                     RALEKHQLCLYYQPIIDIKTEKCIGAEALLRWLGEQGQIMNPAEFIPLAEKEGMIEQI
                     TDYVIDNVFRDLGAYLATHADRYVSINLSASDFHTSRLIARINQKTEQYAVRPQQIKF
                     EVTEHAFLDVDKMTPIILAFRQAGYEVAIDDFGIGYSNLHNLKSLNVDILKIDKSFVE
                     TLTTHKTSHLIAEHIIELAHSLGLKTIAEGVETEEQVNWLRKRGVRYCQGCFFAKAMP
                     PQVFMQWMEQLPARELTRGQ"
BASE COUNT         1835 a         1775 c         1566 g         1481 t
ORIGIN      
        1 tacccgtctc tatcgggtta attttgatga ggctagcgtc cagggaatgg gcatttctat
       61 tctccgtggg gaggaaaata tccccattga tagtgatacc ctggtaacac tacagaaatt
      121 ctttgaagaa gattgtacct ctcatactgg catgtcacaa actgaggata atattaatgc
      181 agtcgctatg aagattactg cagatattat gcaacacgca gattgaggtt aagtcgaaga
      241 acagccaatg aattgacttt acaagagaga taacttattt gttttagaat catgaatgtt
      301 agcccgagcc aacctgttaa aggtgcaaaa gattgcacct ttttcagatt acctcgggcc
      361 tggttacgtc gctttcggct tactgatttt cctcaattag caacgcttca agcaaatcga
      421 gatcgtgcag taatttttgc agcgtttcgt tgctgatctc ccgcgtggcg cgcaggtggt
      481 acagttcagc acgttcagaa cgcaatgccg ccagacggaa gcgacgctca aggttctctt
      541 cctgcacgga actttcaacg tcgttacgtc catcggcgcg acgacgcagg ttaccaatga
      601 cgcgagaact gacctccgta agcagctggt tatcgatatt ctcttcggta tccgccgcca
      661 gacgctcctc cattttctgg atcgccacaa tcgccacttc tgccgttgcc gctcgcgcaa
      721 tacgttcttc tttcaattgt tgcgaatggt ccgcgacttc aatgtgttgt agcagaatag
      781 gcaacatcac cacgccgaca aacagcgaga agagaatgac gccagccgcc aggaacacca
      841 gctcatagcg cgccgggaag acgttaccat ccggcaagag cagcgggata gagagcacac
      901 cggccagagt gatcgcccca cgcaccccgg cgaaagacgc gatcaggatt tctcgtgtgg
      961 tccacgaacc aaactccatc ggcttcttct tcaggaagcg gttgctgaac tttttcatcg
     1021 tccacagcca gccgaaacgg accagcatca gcgccgcata tatcagaata atatcggtaa
     1081 acagcatcca gatttcgacg ttagggtcga tttctgccgc catcagcgac gtctccagaa
     1141 tacccggcag ctgcagacct aacagcagga acaccatgcc gttaaagaca aattccagca
     1201 tcgcccaggt gctgtttgcg cgcaggcgca ttgccagcgg cgcacggcgc atcacaccgg
     1261 agcgggtgat ggtcatcccg gcggcaaccg cagcgaggat gccggaaacg ccaatatgtt
     1321 cggcaatcag ataagaagcg aatggcagca agaacagcag aacgatctgc gtcgcgggtt
     1381 catcaccgcc ccagcggctg aggaatcgca gcgagcgacc atacagccag ctcaccacaa
     1441 agccggcgag aataccgcca atggctactt tcataaattc gaccgtcgca ccgccaacgg
     1501 tgaagatcat cgtccccatc gccactgcca cggcaaactt caacgacacc aggccggagg
     1561 cgtcattcat caacgcttcg ccctgcaaaa tacccatgat ttttttcggg atgcgccctt
     1621 cgccaacaat cccggagagc gccacagcat cggtcggaga aagcactgcc gccagcgcaa
     1681 aggcggggat cagcggaatg cccggcacca cccagtaaat aaggaagcca atgccgacca
     1741 cggtgaccac caccagcgcc agcgcgaggc caaaaatctc tcgaccatgt tcgagaaatt
     1801 cacgggtcgg cgttttccag ccatcagcga acagcaacgg cgggataaac aagactaaaa
     1861 agagttcagg atcaaactcc acatgcaaac caaacgtcgg ccacgccagt agcgcaccga
     1921 tggcgatttg cataagcgga agcgggatct gaaagggcat gacacgagtg accaccccgg
     1981 acagcgagac cacgagggtc attatcagta tggtgaagaa gatttccatg cgttccctgt
     2041 ttgcgatgtt tttttatgat ctgagaaaga cgttatgcct gaattctcat tttttcagaa
     2101 tatcgcaaaa gctcacacaa tgtgcggaga aaataaaaac gggcagccat tgccacccgt
     2161 tttcagaatc cgatgaaaga ttagatagcc cagccacccg catagaaggt caccagcgcc
     2221 acggcgataa cgaccgtacc gatgttcaac ttgcgccatt cgccggaaac cagacgacca
     2281 atcaccagag tcgcgaagcc gatcatgatg cctgttacga tgttacaggt cagcacgatg
     2341 aataccgccg taaccagacc cgccatcgca tcaacaaaat cagcaaagtc gattttcgcc
     2401 acgttgctca gcatcagcag gccaacgtac atcagcgccg gagccgttgc ataccccgga
     2461 acgaggtaag agagcggaga gaggaacaga atcagcagga acagcacgcc aacggtgata
     2521 gccgtcaaac cggttttacc gcccgccgcc gtacccgccg cagactcgat gtataccgct
     2581 gccggagcag cacccaccag gccagagaaa acgctgctca tggagtcagt ggtcagtgct
     2641 ttcccaccgt cgatgatctg cccatcttta tccagcaggt tcgcctggcc ggcgacggca
     2701 cggatagttc cggtggcatc aaatactgcc gtcatcacca gcgccagaac gcttggcagg
     2761 actacaggat tcagcgcgcc cataatgtcc aggctgccaa tcagtgaatt gccgttttca
     2821 tcgctcaatg aaggcatggc gaaaacgccg gagaaatgga cgttaggatc gaagatcaaa
     2881 ccgacaattg agataccgat aatggtcagc agaatgccac cagggacttt cagtttttcc
     2941 aggccgatga tcaccgccag acctactagt gacataatca ccgggaaggt cgcgaaatca
     3001 cccagcgcaa cgggcagacc atcaagcggg tttttaatca ccagaccgac accattagcg
     3061 gcaatgagca gcaggaacag accgataccg atccccgtgc cgtgcgccac accgtgcggc
     3121 aagttgcgca aaatccagct acggataccc gtggcagaaa ttaccgtaaa cagcaccccc
     3181 atcaggaaca cggcacccag cgcgacaggt acgctaatat gttgccccag caccaggctg
     3241 aatgcggtaa acgccgtcag ggagatggcg caaccaatcg ccaacggcag attagcccac
     3301 agacccatca cgatagaacc gagtccggca accagacagg ttgcaacgaa aactgccgca
     3361 ggcgggaagc ctgctttacc caacatacct ggaacgacga tgaccgagta gaccatcgcc
     3421 agaaacgttg ttaacccggc aaccacttcc tgacggacag tgcttccacg ttgtgaaatt
     3481 ttaaaccagg cgtcgagtga accgccggta cgcgctgatg gcgtagacat aagaaaacat
     3541 cccctgagag tttaattttc gtcaagatgc gtggcggaca atcccctgcc agcgaaacgt
     3601 tatcttcctg ctccaggttt gcgacaattt actgcgtcgc aaaaaagcaa acgtttagct
     3661 ccgcgcatac aaaacgggtg gtaaaaaaag caaacgatta tccggcgatc atagcggctt
     3721 tttcaaccga agtttgctgc ttttttctct tttattgtct tgacgatgaa aaaccaggca
     3781 attgatgcgc aaacgttatc gtcggtgcgc aagattgcag cgttatcgag ccgtataccg
     3841 ttcaggattg cgataaaaaa tcgtctgaga tgacaaaaat attgcggata aaaagtatac
     3901 ttttacactg gcgaagaaat gccggtgaca tacaattaaa gcatcaacac caaccggaac
     3961 ctccaccacg tgctcgaatg gggtgtgttg acgtcggggg aaaccctcct gtgtaccagc
     4021 gggatagaga gaaagacaaa gaccggaaaa caaactaaag cgcccttgtg gcgctttagt
     4081 tttgttcatc ttccagcaag cgtgcgccgg taccttcttc tcctaagcgg tcgcccgggt
     4141 tacgcaacgg gcaatcactg cgcgaaaggc agccacaacc aatacatccg tccagttcgt
     4201 cacgcagcgc tactaaggta tgaatgcgcc gatccaactc ttctcgccat tgggacgaaa
     4261 gctgtttcca ctctttcgca cttaacgtat gcccttcggg caacacgcca aacgcttcac
     4321 caatggtcgc cagcggaatg ccaatacgct gagcaatttt gataattgca acatatcgca
     4381 acacatcacg tttatatcgc cgctgattgc cgctgttacg gatactggta atcaaccctt
     4441 tactttcata gaaatgcagc gccgataccg ccacaccgct gcgtttcgcc acttcgccgg
     4501 gggttagcag cgctttaatg cggggtaatt tcttttccat aaatcgcttt acctcaagtt
     4561 aacttgagga attatactcc ccaacagatg aattaacgaa ctgaacactg aaaagaggca
     4621 gatttatgtc ccatcagaaa attattcagg atcttatcgc atggattgac gagcatattg
     4681 accagccgct taacattgat gtagtcgcaa aaaaatcagg ctattcaaag tggtacttgc
     4741 aacgaatgtt ccgcacggtg acgcatcaga cgcttggcga ttacattcgc caacgtcgcc
     4801 tgttactggc cgccgttgag ttgcgcacca ccgagcgtcc gatttttgat atcgcaatgg
     4861 acctgggtta tgtctcgcag cagaccttct cccgcgtttt ccgtcggcag tttgatcgca
     4921 ctcccagcga ttatcgccac cgcctgtagt tttattgccc gcgcgttaac tcccgcgcgg
     4981 gtaattgctc catccattgc ataaacacct gcggcggcat cgccttcgca aaaaaacatc
     5041 cctggcaata gcgcacgccg cgtttgcgca gccagttaac ctgctcctca gtttcgacgc
     5101 cttcagcgat cgtttttaac cccaggctgt gcgccagctc gatgatgtgt tccgcaatca
     5161 aatgactggt tttgtgggtg gtcagcgttt caacaaacga tttatcgatt ttcaaaatat
     5221 cgacattcaa tgatttaagg ttatgcaagt tagagtagcc aataccaaaa tcatcaattg
     5281 ccacttcgta accagcctgg cggaaagcca gaataatcgg cgtcattttg tcaacatcaa
     5341 gaaatgcatg ttcagtcact tcaaatttaa tttgctgcgg acgcaccgcg tattgctctg
     5401 ttttctgatt gattcgcgct atcaaccgtg acgtatgaaa atcggaggcc gacaggttaa
     5461 tagaaacata gcgatcggca tgtgttgcca agtacgcgcc cagatcacgg aagacattat
     5521 caataacata atcagttatc tgttctatca tcccctcctt ttctgccagc ggaataaact
     5581 ctgccggatt cattatttgc ccctgctcac caagccaacg taacaacgct tcagcgccga
     5641 tacatttttc tgttttgata tcgattattg gctggtaata aagacaaagt tgatgttttt
     5701 cgagggcgcg ttgcaattta cgtttgggag ataaatagtt ttgtcgaata cgtagccaga
     5761 gtaatagcag aacaagactc cccaaaatac cggcgggcaa cgcaaaaata agatgattat
     5821 aaaaatgggt tataagacgt tgatatgaag tcgcaacaat ggctgcaatt gggcgttttg
     5881 tcgaataaac tgtcgcatat aaatagccat ttctttgtac ggttaaatcc ttcaaatgaa
     5941 tcagcggaga aaacgttgct gccgaggcct ctttgcttaa cgagaaaaag gttttcgtca
     6001 ccgtatcata cacaccccat tgcaatgtcg gatcatcaga catcacttca ctccagaaga
     6061 gagggttgat aaccgccaca taatttcccc gctgcatata ggtcatttta tagccagaga
     6121 aaaaaggcgt atcgcggtaa taatagatag aaacgttagg ttcacgctta taatcggccg
     6181 gtgcaatcgt atagccgttt acaggcgcta tcagcgatga gcataaaaaa tggttatcac
     6241 gggcatagat caattcatta atataaagat agccacgaat aatattcaac attcgctttt
     6301 gatgggctgg agtgcaaact tgcccctgat agcgttcagc tgcatcgctc actaaatctg
     6361 cctggcgaat gaccagctcg gatttatcca gtgccagttg agcaaaggtt cggagctgat
     6421 tattcacttc tgattttgcc caaaggaagg caatccatag cgaaagaatg atgggaaaga
     6481 gaactaaaaa gataatgccc ggcaacgcca gtaattggtg tcgtgcacga tgactcattg
     6541 ctatcaattt cctgcctgtt tatacccggc cattgcaagg tgaaaaccct gggtaccgac
     6601 gtctttagat aaatcttacg gtataaatag cacttttgcc tgatttgaaa ttgatac
//
LOCUS       DAZKHF020000239         6647 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2201S31241
            SAMN43267800-rid25106413.denovo.241, whole genome shotgun sequence.
ACCESSION   DAZKHF020000239 DAZKHF020000000
VERSION     DAZKHF020000239.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267800
            Sequence Read Archive: SRR30307263
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6647)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6647)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 6647)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350581.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350581.2
            Genome Coverage       :: 151x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:21:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,652
            CDSs (total)                      :: 5,557
            Genes (coding)                    :: 5,320
            CDSs (with protein)               :: 5,320
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 237
            CDSs (without protein)            :: 237
            Pseudo Genes (ambiguous residues) :: 0 of 237
            Pseudo Genes (frameshifted)       :: 102 of 237
            Pseudo Genes (incomplete)         :: 130 of 237
            Pseudo Genes (internal stop)      :: 52 of 237
            Pseudo Genes (multiple problems)  :: 41 of 237
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6647
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267800-rid25106413.denovo.241"
                     /serotype="O 26:H11"
                     /isolate="2201S31241"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-01"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            <1..1413
                     /gene="tssI"
                     /locus_tag="ACHOSC_RS19640"
                     /old_locus_tag="pgaptmp_003928"
     CDS             <1..1413
                     /gene="tssI"
                     /locus_tag="ACHOSC_RS19640"
                     /old_locus_tag="pgaptmp_003928"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system tip protein TssI/VgrG"
                     /protein_id="HGU4549769.1"
                     /translation="EGIFFYEEHAYKSTDQSLVLCDTVRHLPESFEIPWNPNTRTEVS
                     TLCISQFRYSAQIRPSSVVTKDYTFKRPGWAGRFEQEGQHQDYQRTQYEVYDYPGRFK
                     SAHGQNFARWQMDGWRNNAETARGMSRSPEIWPGRRIVLTGHPQANLNREWQVVASEL
                     HGEQPQAVPGRQGAGTALENHFAVIPADRTWRPQPLLKPLVDGPQSAVVTGPAGEEIF
                     CDEHGRVRVRFNWDRYNPADQDSSCWIRVAQAWAGTGFGHLAIPRVGQEVIVDFLNGD
                     PDQPIIMGRTYHQENRTPGSLPGTKTQMTIRSKTYMGSGFNELKFDDATGREQVYIHA
                     QKNMDTEVLNDRTTTVKHDHRETVKNDQTVTIQEGNRLLTVEKGHKITGVLKGSLSED
                     VFQDRGTIAGSVHVDAVNNGGEGDGIQAYTAIKEILLAVEESKIALTPDGIQLQVGES
                     TVIRLSKDGITIVGGSVFIN"
     gene            1441..1902
                     /locus_tag="ACHOSC_RS19645"
                     /old_locus_tag="pgaptmp_003929"
     CDS             1441..1902
                     /locus_tag="ACHOSC_RS19645"
                     /old_locus_tag="pgaptmp_003929"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DcrB-related protein"
                     /protein_id="HGU4549770.1"
                     /translation="MQFTFNEGHIQLPSQWQDQSMQVLVSTDNSGINLVITREAVPQG
                     TLTPELYQETLALYQGKLDGYTEHACREITLAEAPAWLLDYSWNGPEDEGNQGRISQI
                     AVFQRRGDTLLTFTFSTSLSLKNSQKTMLLEVIKSFTPLPPENDIQKDQPR"
     gene            1922..6647
                     /locus_tag="ACHOSC_RS19650"
                     /old_locus_tag="pgaptmp_003930"
                     /pseudo
     CDS             1922..6647
                     /locus_tag="ACHOSC_RS19650"
                     /old_locus_tag="pgaptmp_003930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001289028.1"
                     /note="internal stop; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease fold toxin"
BASE COUNT         1622 a         1801 c         2054 g         1170 t
ORIGIN      
        1 gaaggcatct tcttttatga ggagcatgct tacaaaagta ccgaccagag cctggtgctg
       61 tgcgacacag tccgccatct gcccgaatct tttgaaatcc catggaaccc gaacacccgt
      121 accgaggtga gcaccctctg catcagccag ttccgctaca gcgcacaaat ccgcccttct
      181 tccgtggtga ccaaagacta cacctttaaa cgccccggct gggccggacg ttttgaacag
      241 gaaggccagc accaggacta ccagcgcacg cagtatgaag tgtatgacta ccccggacgt
      301 ttcaagagcg cccacgggca gaactttgcc cgctggcaga tggacggctg gcgaaacaac
      361 gcagaaaccg cgcggggaat gagccgctcg ccggagatat ggccgggacg gcgaattgtg
      421 ctgacggggc atccgcaggc gaacctaaac cgggaatggc aggtggtggc aagtgaactg
      481 cacggcgaac agccacaggc ggtgccagga cggcagggag cggggacggc gctggagaac
      541 cattttgcgg tgatcccggc agacagaaca tggcgaccac agccgttgct gaaaccgctg
      601 gtcgacggcc cgcagagcgc tgtcgtgaca ggaccggcag gcgaggaaat cttctgcgac
      661 gaacatggtc gcgtgcgggt gaggttcaac tgggaccgtt ataacccggc agaccaagac
      721 agttcgtgct ggatccgtgt ggcacaggcg tgggcaggca ccggttttgg ccacctggcg
      781 ataccgcgtg tgggtcagga ggtgattgta gacttcctca acggcgatcc ggaccagccg
      841 atcattatgg ggcgcaccta ccaccaggaa aaccgcaccc ccggcagcct gccgggaaca
      901 aagacgcaga tgaccatccg ctccaaaacg tacatgggca gcggatttaa tgagctgaag
      961 tttgatgatg cgacagggag agaacaggtc tacatccacg cgcagaagaa catggatacc
     1021 gaagtgctca acgaccgtac caccaccgta aaacacgatc accgcgaaac cgtaaaaaat
     1081 gaccagacgg tcacgatcca ggaaggtaac cgccttctta cggtggaaaa aggccacaag
     1141 atcaccggag tactgaaagg gtctttatct gaggatgtct ttcaggacag aggcacgatt
     1201 gccggttcgg tgcatgttga cgctgtaaac aatggtggcg aaggcgacgg tatacaggct
     1261 tatacggcga ttaaggaaat tttgctggct gtggaggaaa gcaaaattgc gctgacgccg
     1321 gatggcattc agctacaggt cggggaatcg acggtaatca ggctgtcgaa ggatggcatc
     1381 accatcgtgg gcggttctgt tttcatcaac tgattcactc agattatcag ggagtatttt
     1441 atgcagttca cttttaatga aggccacatt caactgccgt ctcaatggca ggatcagtca
     1501 atgcaggtcc tggtgtccac agataacagt ggcatcaacc tggtcattac ccgcgaagcc
     1561 gttccacagg gaaccctgac accagagctg taccaggaaa cgctggcgct gtaccagggc
     1621 aaacttgatg ggtacaccga acatgcctgc cgggaaatca ctctggcaga ggccccggcc
     1681 tggctgctgg attattcctg gaacggtccg gaagatgagg ggaatcaggg gcggattagc
     1741 cagatagccg tattccagcg gcgtggagat acgctgctga ccttcacttt cagcacctcc
     1801 ttatccctga agaacagtca aaagacgatg ctgctggagg tgataaagag cttcaccccg
     1861 ttgccaccgg agaatgacat ccaaaaggac cagccgcgct gacataaacg agggataaca
     1921 catgagtgaa ggaccaggcg ggccacaggg agcgaccgca ggcggtacgc tggcaatgcg
     1981 aatgctgtca cagcaggcga tggtcgccag ccagatgaaa cgggcagcca acgacaaagc
     2041 cattgcacag atgctggcag caaagaagtc cggcccacct gccgccaggc tgggcgatga
     2101 aattcagcat aaaagttttc tgggggcact ggcaggggcc gtgctggggg cgatagtgac
     2161 catcgcagaa ggttgcctga ttatggccgc ctgcgccacc ggcccttatg cgctggttct
     2221 ggtgcctgcg ctgatgtatg ccagctataa ggcgagtgat tatgtggagg agaaacagaa
     2281 ccagcttgaa tcatggataa acagcttttg tgacacggac ggcgccatca ataccggttc
     2341 tgaaaatgta aacattaacg gaaagcccgc tgcaagggcc gccgtcaccc ttccccctcc
     2401 tcccccacct ggagcaatac ctgaaatccc acagggggaa ccctcatggg gtgatattgc
     2461 cactgacctg cttgaatcgg cagcggaaaa agcagtacca ctggcgaagg cctgggggaa
     2521 cgctgttatc accctgacgg aaagcaatgc cggttttatg gatcgcgtca gcgccggcgc
     2581 atcgcttctg tttcccgccg gtccggtatt aatggagttt gccaccatgg tgggcgggcg
     2641 tagcgaaatc aaaaaagatg tggatttccc ggaagccggt gaggacacgg cgctctgcga
     2701 caaggagaac aaaccaccga ggatagccca gggcagtagc aacgtcttta tcaacaatca
     2761 gcctgccgcg cgcaagggcg acaaactgga gtgcagtgcg gcgatcgtgg gaggttcgcc
     2821 ggacgtcttt attggcggtg aacaggtgac ctatctggat atccagccgg agttcccgcc
     2881 gtggcagaga atgatcctgg ggggaataac gatagccagc tatcttctgc cgcctgcggg
     2941 actgctggga aaactgaaga atctggcgag actcggcaaa ctgggaaacc tgctggggaa
     3001 aagcgggaag ctgctgggcg caaagctcgg cgcgttgctg gggaaaacag gtaagtcgtt
     3061 aaaaagtatt gccaataaag tcatcagatg ggtaacagat cctgtcgatc cggtaaccgg
     3121 cgcatactgc gacgaacgta ccgacttcac cctgggccag accctccccc tctccttcac
     3181 ccgtttccac agttctgtac tgccgctgca tggcctgacc ggcgtgggct ggagcgactc
     3241 ctggagcgaa tacgcctggg tgcgtgaaca gggaaatcgg gtagatatta tcagccaggg
     3301 agccacgctg aacttcgcct tcgacggtga cagtgatacg gcggttaacc cgtatcacgc
     3361 ccagtacatt ctgcgccgcc gcgacgatta cctggagctg ttcgacaggg atgcactgag
     3421 cagccgcttc ttttatgacg cctttccggg aatgcgtctg cgccacccgg tgactgacga
     3481 taccagcgat gaccgcctgg cacacagccc cgacgaccgg atgtatatgc tgggcgggat
     3541 gagcgacacc gccagcaacc gcatcacgtt tgagcgcgac agccagtacc ggatcacggg
     3601 tgtcagtcac accgacggga tccggcttaa actgacgtac cacgccagcg gctacctgaa
     3661 agccattcac cgcacggata acggcataca gacgctggcg acctacgaac aggatgcgcg
     3721 ggggcggctg acagaagcgg atgcgcggct ggactaccac ctgttttatg agtacgacgc
     3781 tgcggaccgg atcatccgct ggtccgataa cgaccagacg tggagccgtt tcacctacga
     3841 tgcacagggc cggtgcgtga atgtcaccgg ggcggagggc tattacaacg ccacgctgga
     3901 ctatggtgac ggctgcacca ccgtgacgga cggcaagggc actcaccgtt attactatga
     3961 tcctgacggc aatattctgc gggaagaagc gccggacggc agtaccacca cgtatgaatg
     4021 ggatgaattc catcacctgc tggcccgcca ctcccctgcc gggcgggtgg agaagtttga
     4081 atacaacgcc gcactcggtc agttaagccg ttacacggcg gcagacggcg cggagtggct
     4141 gtaccgctat gatgagcgcg gcctgccggt aagcctggtg tcgccacagg gcgaagagac
     4201 ccggctggca tacaccgctc aggggctgct gtcggggata ttccgccagg atgaacggcg
     4261 tctgggcata gagtacgacc accacaaccg gccggaaaca ctcaccgacg tgatgggccg
     4321 tgaacaccac accgaataca gcggtcacga cctgccggtg aagatgcgcg gccccggcgg
     4381 tcagtcagtg cggttacagt ggcagcagca ccataaactg agcggccttg agcgggcaga
     4441 aaccggcgca gaaggattcc gctatgaccg ccacggcaac ctgctggcgt acacggacgg
     4501 taacggcgtt gtctggacaa tggaatacgg cccgttcgat ttgccggtgg cgcgaacgga
     4561 cggtgaaggc caccgctggc agtaccgcta cgataaagac acgctgcagc tcacagaagt
     4621 cattaacccg cagggcgagt cataccgtta tattctggac aactgtggcc gggtgacgga
     4681 agagcgtgac tggggcggcg tggtctggcg ttaccgctat gacgctgatg gcctgtgtac
     4741 cgccagggtc aacggcctgg aggaaaccat cctctacagc cgggacgccg caggccgcct
     4801 ggcagaagtc atcacgccgg aaggcaaaac gcagtatgcg tatgacaaat ccggcaggct
     4861 gacgggtatc ttcagcccgg acggcacatc acagcgcacc ggctatgacg aacgcgggcg
     4921 ggtgaatgtc accactcagg gccgacgggc cattgaatac cactaccccg atgaacacac
     4981 cgttatccgc tgtatcctgc caccggaaga tgaacgcgac agacaccccg atgaatccct
     5041 gctgaaaacc acgtaccgct acaacgccgc cggagaactg acagaggtca ttctgccggg
     5101 ggatgagacg ctgacgttca gccgtgatga ggcgggacgt gaagtgctcc ggcacagtaa
     5161 ccggggtttt gcctgtgaac agggctggaa tgcagccggc cagcctgtca gtcagcgcgc
     5221 cggatttttt ccggaggaaa ccacatgggg cgggctgctc ccctcactgg tacgggagta
     5281 ccgttacgac agcgcgggca atgtgtcggc tgtcaccagc cgggaagatt acggacggga
     5341 aacccggcgg gagtaccggc tggaccggaa cggccaggtc acggcggtga cagcctcagg
     5401 caccgggctg ggctatggcg aaggcgatga gtcctatggc tatgacagtt gtggctacct
     5461 gaaggcgcag tctgcgggca ggcaccggat aagtgaagag actgaccagt atgccggagg
     5521 ccaccggctg aaacaggccg gaaacacgca gtatgactat gacgccgcag gccggatggt
     5581 cagccgcacc agacaccgtg acggctaccg cccggaaaca gagcggttcc ggtgggacag
     5641 ccgggaccag ctgaccgggt attgcagcgc acagggtgag cagtgagaat accgccacga
     5701 cgccagcggc agacgaacag agaaacgctg cgaccggaag aaaatccgct tcacgtacct
     5761 gtgggacggc gacagtattg cggaaatccg ggaataccgc gatgataaac tgtacagcgt
     5821 acggcacctg gtgtttaacg gctttgagct gataagccag cagttcagcc gggtacggca
     5881 ggcgcatccg tcggtggccc cgcagtgggt gacgcggacg aatcatgcgg tgagcgacct
     5941 gacgggccgc ccgctgatgc tctttaacag tgaaggtaaa accgtctggc ggccgggaca
     6001 gaccagcctg tgggggctgg cactcagcct gcccgcagac accggctacc cggacccgcg
     6061 cggggagtgg gacccggaag ccgaccccgg cctgctgtat gcgggacagt ggcaggatgc
     6121 ggaatcgggg ctgtgctata accggttccg gtactacgag ccggaaaccg gaatgtacct
     6181 ggtgagtgat ccgctggggt tgcagggggg tgagcagact taccggtatg tgcctaatcc
     6241 actggggtat attgatccgc tggggctggc aaaaacttct gttcccgcag aaaaaataag
     6301 cctttcggat aaagcaagag atctttttag acaaggaaaa gtaagagaag cattagatgt
     6361 tcattatgag gatcttgtac gaagaaagct tggtggtata tctcaagaaa ttgccgggcg
     6421 tgaatatgat gttgttactg acaagatcat agcgcaggtc aagaggactt atagctctat
     6481 agataatcca aaaaacttct taagtaagtc tactcggaca caaattaaga agacaattga
     6541 gctggctgaa gaacagggga aagaggctca gttttggttt aaatatggag taagccctaa
     6601 agtaagagaa tatatagaaa gcaaaggtgg gaaagttata ttgggta
//
LOCUS       DAZKHF020000240         6606 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2201S31241
            SAMN43267800-rid25106413.denovo.242, whole genome shotgun sequence.
ACCESSION   DAZKHF020000240 DAZKHF020000000
VERSION     DAZKHF020000240.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267800
            Sequence Read Archive: SRR30307263
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6606)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6606)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 6606)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350581.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350581.2
            Genome Coverage       :: 151x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:21:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,652
            CDSs (total)                      :: 5,557
            Genes (coding)                    :: 5,320
            CDSs (with protein)               :: 5,320
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 237
            CDSs (without protein)            :: 237
            Pseudo Genes (ambiguous residues) :: 0 of 237
            Pseudo Genes (frameshifted)       :: 102 of 237
            Pseudo Genes (incomplete)         :: 130 of 237
            Pseudo Genes (internal stop)      :: 52 of 237
            Pseudo Genes (multiple problems)  :: 41 of 237
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6606
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267800-rid25106413.denovo.242"
                     /serotype="O 26:H11"
                     /isolate="2201S31241"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-01"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            <1..384
                     /locus_tag="ACHOSC_RS19655"
                     /old_locus_tag="pgaptmp_003931"
     CDS             <1..384
                     /locus_tag="ACHOSC_RS19655"
                     /old_locus_tag="pgaptmp_003931"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6645 domain-containing protein"
                     /protein_id="HGU4549771.1"
                     /translation="LGIYLYTDVALPDVVAMTGTGNPFLMSFFTQTTDGKLNLMHHKK
                     AGNTKLGEFGNYSNDWQTLELVFTASSATVTPKLNGVAGPAFQVIKDSLTLGLNALTL
                     TDITKNATYGVEIESLVLEINSPAA"
     gene            408..875
                     /locus_tag="ACHOSC_RS19660"
                     /old_locus_tag="pgaptmp_003932"
     CDS             408..875
                     /locus_tag="ACHOSC_RS19660"
                     /old_locus_tag="pgaptmp_003932"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000920596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HGU4549772.1"
                     /translation="MLKTNSLRKGMIHGCRWCQANPEKFTIFVESGNIETTGETPSFV
                     YRYQMVMFVMDYAGELDDLTLPLLAWLSENQPQLLLNPERNQDIKFSAVINDDDSADL
                     LFTLPLRERVRITRSSQGAPQAEHLQEPKPRLPSSEGDWSHVFQDVTWGESDG"
     gene            868..1503
                     /locus_tag="ACHOSC_RS19665"
                     /old_locus_tag="pgaptmp_003933"
     CDS             868..1503
                     /locus_tag="ACHOSC_RS19665"
                     /old_locus_tag="pgaptmp_003933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000356302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016032 - viral process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage virion morphogenesis protein"
                     /protein_id="HGU4549773.1"
                     /translation="MDKAFTRVDETFEAIRDSLNQQAINNIARKLAQDLRRAQQARIR
                     SQKAPDGTAWTPRRRRVTRIQERIRFIWNNEARTLKNWHHDTGKYGRTITGWDEDKNN
                     IRTFYRDDIDRFLEIRTRRINQDSTKRVPMFVKLRTARYLKARADASGVTVGYSGVAA
                     RIARVHQFGERDQVAPGIFTDYPVRELLGISQADERLIYNTVLGRIAEAVR"
     gene            1515..2081
                     /locus_tag="ACHOSC_RS19670"
                     /old_locus_tag="pgaptmp_003934"
     CDS             1515..2081
                     /locus_tag="ACHOSC_RS19670"
                     /old_locus_tag="pgaptmp_003934"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001295912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage baseplate assembly protein V"
                     /protein_id="HGU4549774.1"
                     /translation="MRLLSNIIRTGIISEVDEKSWCVRVRSGELETGWLRWNTTRAGA
                     FNVWLPPSPGEQVVIACIGGNPETAMIIGSLWSDANPAPGKSLKEIVVSAPDGAVFRY
                     DADAGALSASGMKTATLQASVSVTLDTPVVECTDLLRTATLDVTKGGKMSGNITHSGG
                     NFTSNGITVHTHKHGGVKGGSDSTGGPQ"
     gene            2078..2428
                     /locus_tag="ACHOSC_RS19675"
                     /old_locus_tag="pgaptmp_003935"
     CDS             2078..2428
                     /locus_tag="ACHOSC_RS19675"
                     /old_locus_tag="pgaptmp_003935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000213452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GPW/gp25 family protein"
                     /protein_id="HGU4549775.1"
                     /translation="MTTRYTGMNPDGTGNLNDMEHLKQSVRDILTTPLASRVMRREYG
                     SLVPDLIDEPMNNTTRLQCMSAAVIALTRWEPRIALDAIDVVWKAGGRAGVTLSGTVM
                     QTMQNVELTITLRE"
     gene            2432..3328
                     /locus_tag="ACHOSC_RS19680"
                     /old_locus_tag="pgaptmp_003936"
     CDS             2432..3328
                     /locus_tag="ACHOSC_RS19680"
                     /old_locus_tag="pgaptmp_003936"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001297572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="baseplate J/gp47 family protein"
                     /protein_id="HGU4549776.1"
                     /translation="MPAVDLSQLPEPAIIAEPDFEAILADTKAMMIASYPAEQREAVS
                     AALELESEPLNVIAQTTAFREMLLRQRVNEGARACMLSHSAGTDLDNLAGNMNTKRLT
                     ITPATDTTDAVMESDTSLRLRAQRAYDGLSVAGPSGAYEYFARSASGLVRDARAISPS
                     PANVTVSILSTEGDGTATEALLNTVRAVLNAEDTRPVADRLTVQSARIVTWRLNAKLY
                     FYPGPESEPILAAAESSFRKWLAEQGLIGQDVALSAIAAALHVHGVQRVEIIEPTQNM
                     AISDIQAARCESFTISEGGRNE"
     gene            3321..3851
                     /locus_tag="ACHOSC_RS19685"
                     /old_locus_tag="pgaptmp_003937"
     CDS             3321..3851
                     /locus_tag="ACHOSC_RS19685"
                     /old_locus_tag="pgaptmp_003937"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000071700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016032 - viral process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein I"
                     /protein_id="HGU4549777.1"
                     /translation="MSNSLLPPSASSFMRCAEAVGTRITDIPVDLNTLWSPDTCPVHL
                     LPYLAWAFSVDRWDRNWPEETKRQVIRDAWLIHRHKGTISALRRAIEPLGYLIRVSEW
                     WEFGGEPGTFTVEVGTLDSGVTEEMYLEMERLIADARPVSRHMTGLNIIQEIPGDIFA
                     AAATHDGEVITIYPDD"
     gene            3854..4567
                     /locus_tag="ACHOSC_RS19690"
                     /old_locus_tag="pgaptmp_003938"
                     /pseudo
     CDS             3854..4567
                     /locus_tag="ACHOSC_RS19690"
                     /old_locus_tag="pgaptmp_003938"
                     /inference="COORDINATES: protein motif:HMM:NF023985.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
     gene            5132..5986
                     /locus_tag="ACHOSC_RS19695"
                     /old_locus_tag="pgaptmp_003939"
     CDS             5132..5986
                     /locus_tag="ACHOSC_RS19695"
                     /old_locus_tag="pgaptmp_003939"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HGU4549778.1"
                     /translation="MQGATGLELYEAYNNGYPTTYGNIIHLKGMTAVGEGELLIGWSG
                     ISGAHAPAFIRSRRDTPDANWSPWAQLYTSAHPPEEFYPVGAPIPWPSDTVPSGYALM
                     QGQTFDKSAYPKLAVAYPSGVIPDMRGWTIKGKPGSGRAVLSQEQDGIKSHTHSASAS
                     STDLGTKTTSSFDYGTKTTSSFDYGTKTTNSAGNHSHNIPVGHTGAGNGVSAGYNAAL
                     GTGTTSSAGEHAHNVYIGAHNHTIGIGAHAHSVIIGPHGHTITVNATGNEENTVKNIA
                     FNYIVRLA"
     gene            5986..6564
                     /locus_tag="ACHOSC_RS19700"
                     /old_locus_tag="pgaptmp_003940"
     CDS             5986..6564
                     /locus_tag="ACHOSC_RS19700"
                     /old_locus_tag="pgaptmp_003940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000144011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0098004 - virus tail fiber assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tail fiber assembly protein"
                     /protein_id="HGU4549779.1"
                     /translation="MTFRMSEHSRTIKIYNLLTGTNEFIGEGDAYIPPHTGLPANSTD
                     IAPPDIPAGFVAVFNSDEASWHLVEDHRGKTVYDVASGDSLFISELGPLPENVTWLSP
                     YGEYQKWNGTSWVKDAEAEKLFRIREAEETKNSLMQVASEHIAPLQDAVDLDIATEEE
                     ASLLAAWKTYRVLLNRVNTAVAADVEWPVAPQ"
BASE COUNT         1681 a         1703 c         1861 g         1361 t
ORIGIN      
        1 ctgggaattt atctgtatac cgatgtagcg ttacctgacg tcgtggcgat gaccgggact
       61 ggtaacccgt tcctgatgtc gttcttcacc cagaccacag acggcaaact gaatctgatg
      121 catcacaaga aagcaggaaa cacaaagttg ggcgagttcg ggaattacag taacgactgg
      181 cagacgctgg agctggtgtt caccgccagc agtgccacgg ttactccgaa actgaatgga
      241 gtggctggcc cggcattcca ggtcataaaa gacagtctga cactggggct gaatgcactg
      301 acgctgacgg atattaccaa aaatgcaacg tatggcgttg agatagaaag tctggtgctg
      361 gaaataaata gtcctgcagc gtaaggatga acaggagagc aaaacagatg cttaagacaa
      421 acagcctccg gaagggcatg attcatgggt gtcgctggtg tcaggccaac ccggaaaaat
      481 tcaccatttt cgtggagagc ggcaacattg aaacgaccgg agaaacgccc tcgtttgttt
      541 accgctatca gatggtgatg tttgtcatgg attacgccgg ggagctggac gacctcacgc
      601 tgccgctact ggcgtggtta tccgaaaatc agccgcaatt gttgcttaat ccggagcgta
      661 atcaggacat caaattctcc gccgttatca atgacgatga cagcgccgat ctcctgttta
      721 cgctccctct gcgggaacgc gttcgcatca cgcgcagcag tcagggggca ccgcaggcag
      781 aacacctgca ggagccaaaa ccccgtctgc catcttccga aggcgactgg tcgcatgtat
      841 tccaggatgt gacgtggggt gaaagcgatg gataaggcat tcacccgcgt ggatgaaacc
      901 tttgaggcta tccgcgacag cctgaatcag caggccatca ataacatcgc cagaaagctg
      961 gcacaggatt tacgccgcgc ccagcaggcg cgtatccggt cacagaaagc gccggacggg
     1021 accgcgtgga caccacgcag acgccgcgta acccggatac aggagcgcat tcgctttatc
     1081 tggaataacg aagcacgcac gctgaaaaac tggcatcacg acacggggaa atacgggcga
     1141 accattaccg ggtgggatga ggataaaaac aatatccgca cgttttaccg ggatgacatc
     1201 gaccgctttc tggaaatacg cacccggcgc atcaaccagg acagcacaaa gcgcgtcccc
     1261 atgttcgtaa aactgcgcac cgcccgctac ctgaaagccc gtgcagatgc ttccggtgtg
     1321 acggtgggtt acagcggcgt ggccgcacgt attgcccgcg ttcatcagtt cggtgagcgc
     1381 gatcaggttg cgccgggcat tttcaccgat tacccggtac gtgagctgtt gggcatcagt
     1441 caggcagatg aacgcctgat ttataacacg gtgctgggcc ggattgcgga ggctgtacgg
     1501 tgagcgcaga actcatgcga ctgctgagca acatcatccg cactgggatc atctctgaag
     1561 ttgatgagaa gtcctggtgc gtgcgcgttc gcagcggcga actggaaaca ggctggttgc
     1621 gctggaacac cacgcgcgcg ggagccttca atgtgtggct gccgccatca cccggcgaac
     1681 aggtggtaat tgcctgcatt ggcggcaacc cggaaaccgc catgataatt ggcagcctgt
     1741 ggagtgatgc caatccggcc cccggcaaaa gcctgaaaga aatcgtggtc agcgcgccgg
     1801 atggcgcggt gttccgctac gacgcggacg caggcgcact gagcgccagc ggcatgaaaa
     1861 cggccaccct gcaggcatcc gtcagtgtga cactggacac gcccgtcgtg gaatgcacag
     1921 accttctgag aacggcgacg cttgacgtca caaaaggggg aaagatgagc ggcaatatca
     1981 cgcacagcgg cggcaatttc acctcaaacg gcatcacagt gcatacgcat aaacacggtg
     2041 gcgttaaagg tggcagcgat tcgacaggag gcccgcagtg acaacccgct acacaggaat
     2101 gaacccggac ggaacgggaa acctgaacga tatggagcat ctgaaacagt cagtcaggga
     2161 catcctgacc accccgctgg caagccgggt tatgcgacgg gaatatggca gccttgtgcc
     2221 cgatttaatt gacgaaccca tgaataacac cactcgtctg caatgcatga gtgctgccgt
     2281 gattgcgctg acacgatggg aaccccgcat tgccctggac gccatcgacg ttgtctggaa
     2341 ggcaggaggc cgcgccgggg tgacgctgtc gggcactgtc atgcagacca tgcagaatgt
     2401 tgaattaacc atcacgctga gggagtaaat catgcctgcc gttgaccttt cccagttacc
     2461 ggaacccgcc atcatcgcgg agcctgactt tgaggcaatt ctggctgaca caaaggccat
     2521 gatgattgcg tcctatcctg ccgaacagcg tgaagccgtc tccgccgcgc tggagctgga
     2581 atcggaaccc ctgaacgtta tcgcccagac aacagcgttt cgtgaaatgc tgttacgcca
     2641 gcgggtcaat gagggggcac gcgcctgcat gttaagccac agcgccggga cagacctgga
     2701 caacctcgcg ggcaatatga acacaaagcg cctgaccatc actccggcaa cggataccac
     2761 cgacgcagtg atggaaagtg acacctcgct gagactgcgg gcgcagcggg cgtacgatgg
     2821 cctgagtgtt gctggcccgt caggtgcata cgagtatttt gcccgcagcg ccagcggtct
     2881 ggtgcgtgat gcgcgggcta tcagtccgtc tccggcaaat gtgacggttt ccatcctgtc
     2941 cactgaaggc gacggcacag caacggaggc gttgcttaat accgttcgcg ccgttctgaa
     3001 tgcagaggat acccgcccgg tggccgaccg cctgacggta cagagcgcca gaatcgtgac
     3061 atggcggctg aatgcaaaac tgtactttta ccccggcccg gaatccgaac ctattctggc
     3121 ggcggctgaa tcgtcgttca ggaagtggct ggctgagcag gggcttatcg gtcaggacgt
     3181 ggcgttgtcc gccattgctg ccgcactgca tgtgcacggt gtgcaacgcg tggagataat
     3241 cgaacccaca cagaatatgg ccatcagcga catacaggcg gcgcgctgtg agtcgttcac
     3301 catcagcgaa ggtgggcgca atgagtaatt cactgttacc accatcagcc agcagtttca
     3361 tgcgttgtgc cgaagctgtc ggaacgcgca ttacagacat cccggtagac ctcaacacgc
     3421 tgtggtcgcc ggacacctgc ccggtgcacc tgctgcctta tctcgcctgg gcattttccg
     3481 ttgaccgctg ggatcgcaac tggccggaag agacaaaacg acaggtgatt cgtgatgcat
     3541 ggctgataca ccgacacaaa gggaccatca gcgcactgcg cagggccatt gagccgctgg
     3601 gatacctcat tcgcgtgtct gagtggtggg agttcggcgg agaaccggga acatttaccg
     3661 ttgaagtcgg cacactggac agtggcgtga cggaggaaat gtatctggaa atggagcggt
     3721 tgattgctga tgcccgtccg gtcagccgcc acatgacagg gctgaatatc attcaggaaa
     3781 ttccggggga tatttttgca gcggcggcaa ctcatgacgg tgaagttatt accatttatc
     3841 cggacgatta agcatgagta ccacaacacg aaaatttaaa accgttatca ccgatacagg
     3901 tgccaaaaaa ttagctcagg cagccgcgcc agatggtaag cctgtccgcc tgactcatat
     3961 ggccgtgggc gacggtggcg gcgcgttgcc cacaccagac agtaagcaga cccgtctggt
     4021 gcatgaggtg tggcgacaca ctgttaatcg cgtcatcctg gacgcaacac atcagaaccg
     4081 cattattgcg gagctggtta ttcctccaga aacgggcgga ttctggatcc gggaaattgg
     4141 tgtgtttgat gagcacggcg atttaatcgc ggtgggcaat actgccgaaa gttacaagcc
     4201 agccgttgcc gaagggtccg gacgtgcaca aacatttcgc accattctga ccgtatccag
     4261 cactgccact gtggcgctta ccgtggataa caccatggtg atggccacag tggattacgt
     4321 ggataacgaa ctgaaagagc atgaacagtc acgatgtcac ccggatgcct cgctgaccgc
     4381 aaaaggcttt gttcaactca gtagcgccac taacagcgat tctgaaacgc tggctgcaac
     4441 gccgaaagcg gttaaggtcg cgtatgatct tgctaacgga aaatatacag cgcaggatgc
     4501 cacaacagcg cgaaaaggcc ttgtccagct tagtagtgca accaacagta catctgaaac
     4561 gctggcggca acatcaaatg cagtaaaagc tgcctatgac aatgctgaaa aacgtctgca
     4621 gaaagctaag aatggtgagg atatctctga taaagacacc tttacgaaaa atatcggtgc
     4681 ctgccgtgca tatagtgcag agctgaatat tggtggagat agtgaagcat ggacaactgc
     4741 gcagttgatt ttttggctag agagtcaggg ggcatttaac catccttact ggatgtgcaa
     4801 aggctcatgg gcttatgcaa ataataaggt cattacagat acaggttgcg gaagtatttg
     4861 tcttgcaggt gctgttgtgg aagttattgg cacccgcggc gcaatgacca tacgcattac
     4921 cacaccgagt acatccagcg gtgaaggcat ccctaatgct caatttactt atattaatca
     4981 tggtgatgct tatgctcctg gctggcgaag ggactataac tccaggaata agccaacagc
     5041 atcagagatc ggggcgttac cgtcagatgg gacagcagta tcgtcagtta atctggcttc
     5101 aaaaggtcgg ctgaccgccc tgacagataa tatgcagggg gccacaggtc tggagttata
     5161 cgaggcgtat aacaacggat atccaacaac gtatggaaat atcattcacc tgaaagggat
     5221 gacagccgtt ggcgaaggcg aattactcat cggctggagt ggtataagcg gtgctcatgc
     5281 tccggcattt attcgttcac gacgggatac gcccgacgca aactggtcgc cgtgggcgca
     5341 gctttacacc tcggctcatc ctcctgaaga gttttatcca gtcggtgcac cgattccgtg
     5401 gccatcagat accgttccgt ctggttatgc cctgatgcag gggcagactt ttgacaaatc
     5461 tgcatacccg aaacttgcag tcgcttatcc gtcaggcgtt attcctgata tgcgtggctg
     5521 gacgattaag ggcaagcccg gcagtggtcg tgccgtattg tctcaggaac aggacggcat
     5581 taaatcgcac acccacagcg ccagcgcatc cagtacggat ttggggacga aaaccacatc
     5641 gtcgtttgat tacggtacta aaacgaccag ttcatttgat tacggcacaa aaactacgaa
     5701 tagcgctgga aatcattcac acaatatacc tgttggtcac actggcgcgg ggaatggtgt
     5761 atcagccggt tataacgctg cgttaggtac tggtaccacg tcgagcgcag gcgagcatgc
     5821 tcacaatgta tatatcggtg cccataacca cactatcggc attggtgctc atgcccattc
     5881 tgtcattatt ggtccccacg gacacaccat caccgttaac gctacgggta acgaagaaaa
     5941 caccgtaaaa aacatcgcat ttaactatat tgtgaggctt gcataatgac attcagaatg
     6001 agtgaacact cacggaccat aaaaatttat aatctactga ccggaaccaa tgagtttatt
     6061 ggtgaaggtg atgcatacat tccacctcat acaggtctgc ctgcaaacag taccgatatt
     6121 gccccgccag atattccggc tggcttcgtg gccgttttca acagtgatga ggcatcgtgg
     6181 catctcgttg aagaccatcg gggtaaaacg gtttatgacg tggcatcagg ggactcgtta
     6241 tttatttctg aactcggtcc attaccggaa aatgttacct ggttgtcgcc gtatggagag
     6301 tatcagaagt ggaacggcac atcctgggtg aaagatgcag aagcagaaaa actgtttcgg
     6361 ataagggagg cggaagaaac aaaaaacagc ctgatgcagg tagccagcga gcatattgcg
     6421 ccacttcagg atgccgtaga tttggatatt gcaacggagg aagaggcatc gttactggct
     6481 gcatggaaga cataccgggt attgttgaat cgtgttaata cagcggtagc agcggatgtt
     6541 gagtggccag tcgccccaca ataaagagaa aaagccatcg attgaaatat agatggcttt
     6601 atgtac
//
LOCUS       DAZKHF020000241         6525 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2201S31241
            SAMN43267800-rid25106413.denovo.243, whole genome shotgun sequence.
ACCESSION   DAZKHF020000241 DAZKHF020000000
VERSION     DAZKHF020000241.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267800
            Sequence Read Archive: SRR30307263
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6525)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6525)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 6525)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350581.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350581.2
            Genome Coverage       :: 151x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:21:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,652
            CDSs (total)                      :: 5,557
            Genes (coding)                    :: 5,320
            CDSs (with protein)               :: 5,320
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 237
            CDSs (without protein)            :: 237
            Pseudo Genes (ambiguous residues) :: 0 of 237
            Pseudo Genes (frameshifted)       :: 102 of 237
            Pseudo Genes (incomplete)         :: 130 of 237
            Pseudo Genes (internal stop)      :: 52 of 237
            Pseudo Genes (multiple problems)  :: 41 of 237
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6525
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267800-rid25106413.denovo.243"
                     /serotype="O 26:H11"
                     /isolate="2201S31241"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-01"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            <1..512
                     /locus_tag="ACHOSC_RS19705"
                     /old_locus_tag="pgaptmp_003941"
     CDS             <1..512
                     /locus_tag="ACHOSC_RS19705"
                     /old_locus_tag="pgaptmp_003941"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HGU4549780.1"
                     /translation="FTLAWKPGEEGQKWLIGWFESGDVRAYKIRFPNGTVDVFRGWVS
                     SIGKAVTAKEVITRTVKVTNVGKPSVAEERSKITPVTAIKVTPTSGTVAKGKTTTLTV
                     SFEPESATDKTFRAVSADPSKATISVKDMTITVNGVATGKVQIPVVSGNGQFAAVAEV
                     TVTEAGAAG"
     gene            526..948
                     /locus_tag="ACHOSC_RS19710"
                     /old_locus_tag="pgaptmp_003942"
     CDS             526..948
                     /locus_tag="ACHOSC_RS19710"
                     /old_locus_tag="pgaptmp_003942"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage minor tail protein G"
                     /protein_id="HGU4549781.1"
                     /translation="MFLKTEQFEYNGVSVTLSELSALQRFDYIKFVSDAEQQETTKHD
                     VVHINQRYLETASLLVAMSLWHSHSLKGTLASPETEMQQIRREVMLGWPADALNQATN
                     RVLYLSGMLDNRHDADPEQTGKAEATEPVTSKKHSKAS"
     gene            975..1388
                     /locus_tag="ACHOSC_RS19715"
                     /old_locus_tag="pgaptmp_003943"
     CDS             975..1388
                     /locus_tag="ACHOSC_RS19715"
                     /old_locus_tag="pgaptmp_003943"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail assembly protein T"
                     /protein_id="HGU4549782.1"
                     /translation="MGRPDWRAMLAGMTSTEYADWRRFYCTHYFQDTQLDAHFSGLMY
                     AVLSLFFGDPDMHPADFSLLAPACEEEQTEMPDEEEMLMQKATGVAGGVRFGGDGGRD
                     ISPSADVVDVSEDDVALMMASAGIYGGVRYVPASG"
     gene            1369..3981
                     /locus_tag="ACHOSC_RS19720"
                     /old_locus_tag="pgaptmp_003944"
     CDS             1369..3981
                     /locus_tag="ACHOSC_RS19720"
                     /old_locus_tag="pgaptmp_003944"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016032 - viral process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tape measure protein"
                     /protein_id="HGU4549783.1"
                     /translation="MSQPAGDLVIDLSLDAARFDEQMARVRRHFSSLEADARKTASTV
                     EQGLSRQALAAQKAGISVGQYKAAMRTLPAQFTDIVTQLAGGQNPFLIMLQQGGQISD
                     SFGGPLSLLTLLKEELLGIRDASESSEESLSDTANALAENARNAGELGRFMSVARVAA
                     GGGVAVLAALAAAAWQAEQADRALLRSLILTGGAAATTTAELWKMAGVISDEAGGGIR
                     QAAENLARLAESGKYTAGQLRIMGETSQRWLQTVGDDAGKVEKAFEGIAADPVKALAS
                     LNQQYNFLSVSQLRHIDELERTKGKQAAVTEAMSLFADVMNARLEQLDKAATPVEKIW
                     DDVKTWTSDAWAWIGDHTLGALSLITDVVAGTVEQVKLLLVQGDLALAEFIQSAWETT
                     KNVPGVGALFGELAEENRVFIEKTKRDELALRKSIAERDARIRQGEMGYINRSRATGV
                     SKGPGQQEAVSRLAEELTGKKHTSPKTRSAGEREEEQAREALLALEAELRTLEKHSGA
                     NEKISRQRRDLWKAESQYAVLKEAATKRQLSEQEKSLLAHKDETLEYKRQLAELGDKV
                     EYQKRLNELAQQAVRFEEQQSSKQAAISAKARGLTDRQAQRESEAQRLRDVYGDNPAA
                     LAKATSALKNTWSAEEQLRGSWMAGLKSGWGEWAESATDSFSQVKSAATQTFDGIAQN
                     MAAMLTGAEADWRGFTRSVLSMMTEILLKQAMVGIVGRIGSAIGGAFGGGASASSGTA
                     IEAAAANFHFATGGFTGTGGKYEPAGIVHRGEFVFTKEATSRIGVGNLYRLMRGYAEG
                     GYVGGAGSPAQMRRTEGINFNQNNHVVIQNDGTNGQAGPQLMKAVYDMARKGAQDELR
                     LQLRDGGMLSGSGR"
     gene            3978..4307
                     /locus_tag="ACHOSC_RS19725"
                     /old_locus_tag="pgaptmp_003945"
     CDS             3978..4307
                     /locus_tag="ACHOSC_RS19725"
                     /old_locus_tag="pgaptmp_003945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HGU4549784.1"
                     /translation="MKTFRWKVKPDMEVNSQPSVREVRFGDGYSQRMAAGLNADLKTY
                     RVTLSVTREEARHLEAFLAEHGGWKAFLWKPPYAYRQIKVTCAGWSARVGMLRVEFSA
                     EFKQVVN"
     gene            4307..5005
                     /locus_tag="ACHOSC_RS19730"
                     /old_locus_tag="pgaptmp_003946"
     CDS             4307..5005
                     /locus_tag="ACHOSC_RS19730"
                     /old_locus_tag="pgaptmp_003946"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030430 - host cell cytoplasm [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0046718 - symbiont entry into host cell
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage minor tail protein L"
                     /protein_id="HGU4549785.1"
                     /translation="MQDIHEESLNESVKSEQSPRVVLWEIDLTAQGGERYFFCNELNE
                     KGEPVTWQGREYQAYPIEGSGFEMNGKGSSARPSLTVSNLFGLVTGMAEDLQSLVGAT
                     VVRRRVYARFLDAVNFVAGNPEADPEQELTDRWVVEQMSSLTAMTASFVLATPTETDG
                     ALFPGRIMLANTCMWDYRGDECGYNGPAVADEFDNPTTDIRKDRCSKCMRGCEMRGMV
                     ANFGGFLSINKLSQ"
     gene            5010..5753
                     /locus_tag="ACHOSC_RS19735"
                     /old_locus_tag="pgaptmp_003947"
     CDS             5010..5753
                     /locus_tag="ACHOSC_RS19735"
                     /old_locus_tag="pgaptmp_003947"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C40 family peptidase"
                     /protein_id="HGU4549786.1"
                     /translation="MTETESAILAHARRCAPAESCGFVISTPEGDRYQPCVNISAEPE
                     AYFRIAPEDWLRAEMQGEIVALVHSHPGGLPWLSEADRRLQIKSALPWWLVCRGDIHK
                     FRCVPHLTGRRFEHGVTDCYTLFRDACHLAGITLPDFHREDEWWRNGQNLYLDNMEAT
                     GFYRVPLSSAQAGDILLCCFGASVPNHAAIYCGNGELLHHLPEQLSKRERYSEKWQRR
                     THSVWRHRHWHVSAFTGIYNDLAAASACT"
     gene            5750..6328
                     /locus_tag="ACHOSC_RS19740"
                     /old_locus_tag="pgaptmp_003948"
     CDS             5750..6328
                     /locus_tag="ACHOSC_RS19740"
                     /old_locus_tag="pgaptmp_003948"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310189.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tail assembly protein"
                     /protein_id="HGU4549787.1"
                     /translation="MNTAAEAIRALSLQMPGFRRQMNEGWYQIRIRGEDTAPEAVYAR
                     LHEPLGEGAVIHIVPRLAGAGKGGLQIVLGAAAIVGSFFTAGATMALWGAALSAGGLT
                     ATTMLFSLGASMILGGVAQMLAPKAKTPDYRATDNGKQNTYFSSLDNMIAQGNPMPVP
                     YGEMLVGSRRISQDISTRDEGGDGKVVVIGRG"
BASE COUNT         1486 a         1547 c         2170 g         1322 t
ORIGIN      
        1 gttttacgct ggcctggaaa ccgggagaag aaggtcagaa atggcttata ggctggtttg
       61 aaagcgggga tgtgcgggcc tataaaatcc gtttcccaaa tggcacggtg gatgtgttcc
      121 gtggctgggt cagcagtatc ggtaaggccg tgacggcgaa agaagtgatc acccgcacgg
      181 tgaaagtgac caacgtgggc aaaccttctg tagcggaaga acgcagcaaa attacgccgg
      241 tcactgcgat taaggtgacg ccgacatccg gtacggtggc aaaagggaaa acaaccaccc
      301 tgacggtttc ttttgagccg gaaagtgcaa ccgacaagac gttcagagcg gtttccgccg
      361 atccgtcgaa agccaccatt agtgtgaaag atatgacaat tacggtaaac ggcgtggcga
      421 caggtaaggt gcagatccct gtggtgagcg gaaatggtca gttcgccgca gtggctgaag
      481 tcaccgttac tgaagcgggc gctgcagggt aaacggaggt aatacatgtt tctgaaaaca
      541 gaacaatttg aatataacgg tgtgtccgtc acgctttccg aattgtctgc gctgcagcgg
      601 tttgattata taaagtttgt ttcagacgca gaacaacagg agacaacgaa gcatgatgtc
      661 gtgcacatta accagcgata tctggaaacg gcatccctgc ttgtggcgat gtcgctatgg
      721 cattcccatt ccctcaaagg cactctggcc tctccggaga cagagatgca gcagatccgc
      781 cgtgaagtga tgctgggatg gcctgctgat gcactgaatc aggcaacgaa ccgggtgctt
      841 tatctttcag gtatgctgga taaccggcac gatgccgatc ctgaacaaac cgggaaagca
      901 gaagcgactg agccggtaac atcaaaaaag cattcgaagg cgagctgaac tttgtcctga
      961 aactggcgcg agagatgggg agacccgact ggcgcgccat gcttgccggg atgacatcca
     1021 ccgaatatgc cgactggcga cgtttttact gcacgcatta ttttcaggat acccaactgg
     1081 acgctcattt ttccgggctg atgtacgccg tactcagcct gttttttggc gatccggata
     1141 tgcatccggc ggatttcagt ctgcttgctc cagcgtgtga ggaagagcag acggagatgc
     1201 cggacgagga agaaatgctg atgcagaaag cgacaggagt tgccggaggc gtccggttcg
     1261 gaggggacgg agggcgcgat atttcacctt ctgcggatgt ggtggatgtc agcgaggatg
     1321 atgttgcatt aatgatggct tcagcgggga tttacggagg tgtgagatat gtcccagcca
     1381 gcgggtgatc tggttattga tttgagtctg gatgcggccc ggtttgatga acagatggcc
     1441 cgggtacgcc gtcatttttc cagtctggag gcggatgcca gaaaaaccgc cagtactgtt
     1501 gaacaggggc tgagccgaca ggcgctggcg gcacaaaaag ccgggatatc agtcggacag
     1561 tataaggctg ccatgcgcac actgcccgca cagttcacgg atattgtcac tcagcttgcc
     1621 ggtggtcaga atcccttcct tatcatgctg cagcaggggg ggcagatcag cgattcattc
     1681 ggtggaccgc tcagcctgct taccctgctg aaggaggaac ttctcgggat cagggatgcc
     1741 tctgaatcat cagaggagtc gctgtcagat acggcaaatg cactggctga aaatgcccgg
     1801 aatgccggtg agctgggacg atttatgtcg gtggcccgtg tggcggcagg tggcggggtt
     1861 gccgtactgg ccgcgcttgc tgccgccgcc tggcaggcag agcaggctga ccgggcctta
     1921 ttgcgttcac tgatcctgac cggaggggcg gctgccacca caacggcaga attgtggaaa
     1981 atggccgggg tgatcagcga tgaagccggt ggtggtatca gacaggcggc agaaaatctg
     2041 gcccgtctgg cagaaagcgg gaaatatacc gccgggcagc tacggatcat gggggaaacc
     2101 tctcagagat ggctgcagac ggtgggggac gatgccggga aggtggaaaa agcctttgaa
     2161 gggattgcag cagatccggt gaaggcgctg gcctccctga atcagcagta taacttcctg
     2221 agcgtttccc agttacgcca tattgatgag cttgagcgca cgaaaggtaa acaggctgcg
     2281 gtgacggagg cgatgtccct gtttgcggat gtcatgaatg cacgtctgga gcaacttgat
     2341 aaagcggcca cgccggtgga aaaaatctgg gacgatgtta aaacctggac ttctgacgca
     2401 tgggcatgga taggtgatca tacactgggg gcactcagtc tgatcactga cgtggtggcc
     2461 ggaaccgttg aacaagtgaa gctgctgctt gtgcaggggg atctggcgct ggctgaattt
     2521 attcagtcag cctgggaaac gacaaagaat gtgcccggcg ttggtgcgtt gtttggtgaa
     2581 ctggcagaag agaaccgcgt atttattgag aaaacaaaac gcgatgaact ggcgctgaga
     2641 aaatccattg cggaacggga tgcgcgtata cgccaggggg aaatggggta catcaaccgt
     2701 tcgcgtgcaa caggcgtcag caaaggtccg gggcagcagg aagccgtcag ccgtctggct
     2761 gaagagctga caggtaaaaa gcatacatca ccgaaaacgc gctctgccgg ggagagggaa
     2821 gaggagcagg caagagaggc tctgcttgcc cttgaagctg agctcaggac gctggaaaaa
     2881 cacagcggtg cgaatgagaa aatcagccgg cagcgccgtg atttatggaa ggcggaaagt
     2941 cagtatgcgg tcctgaaaga ggctgccacg aaacgacagt tatctgagca ggaaaaatcc
     3001 ctgctggcgc ataaagacga gacgctggag tacaaacgcc agctggctga gctgggcgac
     3061 aaggttgaat accagaaacg cctgaatgag ctggcacagc aggcggtgcg gtttgaagag
     3121 cagcagagct cgaagcaggc cgccatcagc gcaaaagccc gcggtctcac tgaccgtcag
     3181 gcgcagcggg agtctgaagc gcagcgtctt cgggacgtgt acggtgataa tccggctgcg
     3241 ctggcgaagg ccacatcggc actgaagaac acctggtctg cggaggagca gcttcgtgga
     3301 agctggatgg ccgggctgaa gtccggctgg ggcgagtggg cggaaagtgc gacggacagt
     3361 ttttcgcagg ttaaaagtgc tgccacgcag acctttgacg gtattgcaca gaatatggcg
     3421 gcgatgctga ccggtgcaga ggcagactgg cggggattca cccgttctgt gctgtccatg
     3481 atgacagaaa tcctgcttaa acaggccatg gtgggcattg tcgggcgtat cggcagcgcc
     3541 attggcggtg ctttcggtgg tggtgcatct gcttcctcgg ggacggccat tgaggctgcg
     3601 gcggcgaact tccatttcgc gaccggagga tttacgggga cgggcggcaa atatgagcct
     3661 gcggggatag ttcaccgcgg ggagtttgtt ttcacgaaag aggcaaccag ccggataggt
     3721 gtggggaatc tttaccgtct gatgcgcggc tatgcggaag gtggttatgt gggcggtgcc
     3781 ggaagtccgg cgcagatgcg gcggacggaa ggcattaatt ttaatcagaa caatcacgtg
     3841 gtgattcaga acgacggcac caacggacag gcggggccgc agctgatgaa ggcggtgtat
     3901 gacatggccc gcaagggggc gcaggatgag ctccggctgc agttgcgtga tggcggtatg
     3961 ttatcaggga gcgggcgatg aaaacctttc gctggaaagt gaagccggat atggaggtga
     4021 actcacagcc atcggtgcgt gaagtgcgtt ttggtgacgg gtactcacag cgtatggcgg
     4081 cagggctgaa tgctgacctg aaaacatacc gggtgacgct ttccgtgacc cgggaggagg
     4141 cccggcatct ggaagcgttt ctggcagagc acgggggctg gaaggcattt ttgtggaagc
     4201 caccctatgc ataccggcag ataaaggtga cctgtgccgg gtggtctgcg cgggtcggga
     4261 tgttgcgcgt tgagttcagc gcggagttta agcaggtggt gaactgatgc aggatattca
     4321 cgaagaaagt ctgaacgagt cggttaaatc agagcagtca ccgcgggtgg tactctggga
     4381 aatcgacctg acggcgcagg gcggtgagcg gtattttttc tgcaatgagc tgaatgaaaa
     4441 aggggagccg gtgacctggc aggggcgtga atatcaggcg tacccgattg agggcagcgg
     4501 ctttgagatg aacggaaagg gcagcagtgc ccgcccgtcg ctgacggtgt ccaatctgtt
     4561 cggtcttgtc accgggatgg cggaggattt gcagagcctg gtgggtgcca cggtggtccg
     4621 ccgccgggtg tatgcgcgtt ttctggatgc ggtgaacttt gtggcaggca atccggaggc
     4681 cgacccggag caggagctga ctgaccggtg ggtggtggag cagatgtcat cgctgacggc
     4741 catgacggcc tcgtttgtgc tggcaacacc gacggagacg gacggggcgc tgtttcctgg
     4801 tcgcatcatg ctggcgaaca cctgtatgtg ggattaccgg ggagatgaat gcgggtataa
     4861 cggtcctgcg gtggcggatg agttcgacaa ccccaccacg gatatccgta aggacagatg
     4921 cagcaagtgc atgcgcgggt gtgagatgcg cggcatggtg gctaattttg gcggtttcct
     4981 ttccattaat aaactttcgc agtaaatcca tgacagagac agaatcagcg attctggcgc
     5041 atgcccggcg gtgtgcgcct gcggagtctt gcggcttcgt gataagcacg ccggagggtg
     5101 accggtacca gccgtgcgtg aatatctctg cagagcctga ggcatatttt cgtattgccc
     5161 cggaagactg gctgcgggca gagatgcagg gggagattgt ggcactggtc cacagccatc
     5221 ccggtggcct gccctggctg agcgaggccg accggcggct gcagataaaa agtgccctgc
     5281 cctggtggct ggtctgccgg ggggacattc ataaattccg ctgtgtgccg cacctgaccg
     5341 ggcgtcgttt tgaacacggg gtgacggact gttacacgct gttcagggat gcctgccatc
     5401 tggcggggat aacgctgccg gattttcacc gcgaggatga gtggtggcgc aatggtcaga
     5461 acctgtatct ggacaatatg gaggccactg gtttttaccg ggtgcccctg tcctcggcac
     5521 aggcaggcga tatcctgctg tgctgctttg gcgcatcggt acccaaccat gctgccattt
     5581 actgcggcaa cggtgaactg cttcaccatc tgcctgaaca actgagtaaa cgggagaggt
     5641 attccgaaaa atggcaacga cgaacgcatt ctgtctggcg tcaccgccac tggcacgtat
     5701 ctgccttcac ggggatttac aacgatttgg ccgccgcctc agcctgtacg tgaacacggc
     5761 agcggaagcc atccgtgccc tgtcgctgca gatgccggga ttccgccgtc agatgaacga
     5821 aggctggtac cagatacgta ttcgcggtga ggacacggca ccggaggcgg tgtacgcccg
     5881 tcttcacgaa cctctgggtg agggggcggt catccatatt gtgccgcgac tggccggagc
     5941 cggaaagggc ggactgcaga ttgtgctggg ggcagcagcc atcgtgggct ctttcttcac
     6001 cgccggcgca acgatggcgt tgtggggcgc agccctgagt gccggagggc tgactgccac
     6061 cacgatgctg ttctcactgg gtgccagcat gatactgggc ggtgtggccc agatgctggc
     6121 accgaaggca aaaacaccgg attaccgcgc aacggataac ggtaaacaga acacgtattt
     6181 ttcgtcactg gataacatga ttgcccaggg gaacccgatg ccggtgcctt atggtgaaat
     6241 gctggttggt tcacgacgga tatcccagga catcagcacc cgtgatgagg gcggagacgg
     6301 gaaagtggtg gttatcgggc ggggatgaaa ataaaaaaat cccgcagagt tagcggagct
     6361 gcgggagaga acgatgaaga ttaacgttat ggagttattt ttcaggcatc aaaaaagtaa
     6421 tgcagcgtca ttattgcggc tacaggcaat tgccggaaat gtgaagagtt tcagaaattt
     6481 tattccgtca tgacacaggc accctccggg gtgcctgttg ttttc
//
LOCUS       DAZKHF020000242         6464 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2201S31241
            SAMN43267800-rid25106413.denovo.244, whole genome shotgun sequence.
ACCESSION   DAZKHF020000242 DAZKHF020000000
VERSION     DAZKHF020000242.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267800
            Sequence Read Archive: SRR30307263
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6464)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6464)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 6464)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350581.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350581.2
            Genome Coverage       :: 151x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:21:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,652
            CDSs (total)                      :: 5,557
            Genes (coding)                    :: 5,320
            CDSs (with protein)               :: 5,320
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 237
            CDSs (without protein)            :: 237
            Pseudo Genes (ambiguous residues) :: 0 of 237
            Pseudo Genes (frameshifted)       :: 102 of 237
            Pseudo Genes (incomplete)         :: 130 of 237
            Pseudo Genes (internal stop)      :: 52 of 237
            Pseudo Genes (multiple problems)  :: 41 of 237
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6464
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267800-rid25106413.denovo.244"
                     /serotype="O 26:H11"
                     /isolate="2201S31241"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-01"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            complement(276..1342)
                     /locus_tag="ACHOSC_RS19745"
                     /old_locus_tag="pgaptmp_003949"
                     /pseudo
     CDS             complement(276..1342)
                     /locus_tag="ACHOSC_RS19745"
                     /old_locus_tag="pgaptmp_003949"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312500.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family secretion protein"
     gene            complement(1345..1707)
                     /locus_tag="ACHOSC_RS19750"
                     /old_locus_tag="pgaptmp_003950"
     CDS             complement(1345..1707)
                     /locus_tag="ACHOSC_RS19750"
                     /old_locus_tag="pgaptmp_003950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3302 domain-containing protein"
                     /protein_id="HGU4549788.1"
                     /translation="MFLNYFALGVLIFVFLVIFYGIIAIHDIPYLIAKKRNHPHADAI
                     HTAGWVSLFTLHVIWPFLWIWATLYQPERGWGMQSHVASQEKATDPEIAALSDRISRL
                     EHQLAAEKKTDYSTFPEI"
     gene            2244..4157
                     /gene="mtlA"
                     /locus_tag="ACHOSC_RS19755"
                     /old_locus_tag="pgaptmp_003951"
     CDS             2244..4157
                     /gene="mtlA"
                     /locus_tag="ACHOSC_RS19755"
                     /old_locus_tag="pgaptmp_003951"
                     /EC_number="2.7.1.198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannitol transporter subunit IICBA"
                     /protein_id="HGU4549789.1"
                     /translation="MSSDIKIKVQSFGRFLSNMVMPNIGAFIAWGIITALFIPTGWLP
                     NETLAKLVGPMITYLLPLLIGYTGGKLVGGERGGVVGAITTMGVIVGADMPMFLGSMI
                     AGPLGGWCIKHFDRWVDGKIKSGFEMLVNNFSAGIIGMILAILAFLGIGPIVEALSKM
                     LAAGVNFMVVHDMLPLASIFVEPAKILFLNNAINHGIFSPLGIQQSHELGKSIFFLIE
                     ANPGPGMGVLLAYMFFGRGSAKQSAGGAAIIHFLGGIHEIYFPYVLMNPRLILAVILG
                     GMTGVFTLTILGGGLVSPASPGSILAVLAMTPKGAYFANIAGVCAAMAVSFVVSTILL
                     KTSKVKEEDDIEAATRRMQDMKAESKGTSPLSAGDVTNDLSHVRKIIVACDAGMGSSA
                     MGAGVLRKKIQDAGLSQISVTNSAINNLPPDVDLVITHRDLTERAMRQVPQAQHISLT
                     NFLDSGLYTSLTERLVAAQRHTENEVKVKDSLKDSFDDSSANLFKLGAENIFLGRKAA
                     TKEEAIRFAGEQLVKGGYVEPEYVQAMLDREKLTPTYLGESIAVPHGTVEAKDRVLKT
                     GVVFCQYPEGVRFGEEEDDIARLVIGIAARNNEHIQVITSLTNALDDESVIERLAHTT
                     SVDEVLELLAGRK"
     gene            4387..5535
                     /gene="mtlD"
                     /locus_tag="ACHOSC_RS19760"
                     /old_locus_tag="pgaptmp_003952"
     CDS             4387..5535
                     /gene="mtlD"
                     /locus_tag="ACHOSC_RS19760"
                     /old_locus_tag="pgaptmp_003952"
                     /EC_number="1.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709374.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008926 - mannitol-1-phosphate
                     5-dehydrogenase activity [Evidence IEA];
                     GO_process: GO:0019594 - mannitol metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol-1-phosphate 5-dehydrogenase"
                     /protein_id="HGU4549790.1"
                     /translation="MKALHFGAGNIGRGFIGKLLADAGIQLTFADVNQVVLDALNARH
                     SYQVHVVGETEQVDTVSGVNAVSSIGDDVVDLIAQVDLVTTAVGPVVLERIAPAIAKG
                     LVKRKEQGNESPLNIIACENMVRGTTQLKGHVMNALPEDAKAWVEEHVGFVDSAVDRI
                     VPPSASATNDPLEVTVETFSEWIVDKTQFKGALPNIPGMELTDNLMAFVERKLFTLNT
                     GHAITAYLGKLAGHQTIRDAILDEKIRAVVKGAMEESGAVLIKRYGFDADKHAAYIQK
                     ILGRFENPYLKDDVERVGRQPLRKLSAGDRLIKPLLGTLEYSLPHKNLIQGIAGAMHF
                     RSEDDPQAQELAALIADKGPQAALAQISGLDANSEVVSEAVTAYKAMQ"
     gene            5535..6122
                     /gene="mtlR"
                     /locus_tag="ACHOSC_RS19765"
                     /old_locus_tag="pgaptmp_003953"
     CDS             5535..6122
                     /gene="mtlR"
                     /locus_tag="ACHOSC_RS19765"
                     /old_locus_tag="pgaptmp_003953"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol operon repressor MtlR"
                     /protein_id="HGU4549791.1"
                     /translation="MVDQAQDTLRPNNRLSDMQATMEQTQAFENRVLERLNAGKTVRS
                     FLITAVELLTEAVNLLVLQVFRKDDYAVKYAVEPLLDGDGPLGDLSVRLKLIYGLGVI
                     NRQEYEDAELLMALREELNHDGNEYAFTDDEILGPFGELHCVAALPPPPQFEPADSSL
                     YAMQIQRYQQAVRSTMVLSLTELISRISLKKAFQK"
     gene            complement(6134..6343)
                     /gene="yibT"
                     /locus_tag="ACHOSC_RS19770"
                     /old_locus_tag="pgaptmp_003954"
     CDS             complement(6134..6343)
                     /gene="yibT"
                     /locus_tag="ACHOSC_RS19770"
                     /old_locus_tag="pgaptmp_003954"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YibT"
                     /protein_id="HGU4549792.1"
                     /translation="MGKLGENVPLLIDKAVDFMASSQAFREYLKKLPPRNAIPSGIPD
                     ESVPLYLQRLEYYRRLYRPKQVEGQ"
BASE COUNT         1614 a         1641 c         1660 g         1549 t
ORIGIN      
        1 taaagccatc atcgcaatag atggaaaagc actcctgctc ttttttgatt agagccgtcg
       61 caggaggaac aactcatatc atcattgcga tctcgacgat gtatagtctc atcctgcggc
      121 agaacaagac ggataaaaaa tccaatgaga gcacactcat ccagaactta ttaacataat
      181 gaatattaaa gaaaaatcat cgcagctaat gaagtaatat gcaaaaataa ttagtcctta
      241 caaggagcaa agttccataa tctcccccct ccccctcaat gatccaaata aagataatgc
      301 atccagctgg tcattcttag cagcactttc cgcatcaccg aaacatggct gaaatggtcg
      361 gagtaaaccg ccacctgggc gtagatgccg tcgggtaagg catcgatatc atcgttaggg
      421 tccagttcaa tggttcccag cacaccgtcc gtgccgggca cgaccgttaa tgattgcaat
      481 accccctgcg cctgataaga accgcctggc acgacaggta aaatactggt cagtttgccg
      541 tggaacacct gcccaggtag cgcgttaaac accacttctg catcatcacc aggtttcaga
      601 cgtaacagcg agttttgccg aaattgggcg acaatttgcc gtttttgctc ggggatgaaa
      661 accatcaccg gacgcaacgg caaggcagct gcgtatgtac ctgggcggat cagtacctga
      721 gtaatgtagc cattgctcgg cgcgcgaatg acagtctgct caagattata ttttgcttca
      781 gtaagttgcg ctcttaagct cacaatctgc gattgctcgc cgttaaccat actgtcgagc
      841 tggctctgga tctgcgcctg ctccgccacc gagcctttca ccagcgcatc ctgcgcgagg
      901 aaattttgcc gcgcatcgtc gatgtcacgt tccgagaacg gattcaccgc cgcctggcta
      961 cctttcagat aacgttgata atttttaaac agacggtcgc gctccgctga aacctgggtg
     1021 gtgttggcct gcgcttctgt gagctgcgcg cgcagagtct ttatattatg cgtcgccgtc
     1081 atcaggtcag cctgaagcct gtcaactcgc gcctggtaac gaaccgggtc gagcttaaaa
     1141 agcacctcgc ccttttgaat aagctgatta ttcttgtcag tgacttcagt aacaattccc
     1201 gtcacctgtg gcgtgatggg gatcgctatc actgcctttt gcgcggtaaa agtgtaaggg
     1261 tggttgtagt tcatcaacaa aatcaaacca ctcaccagaa acacgccagc gccacgtaag
     1321 ttaaaacaat caatagatcc ataattagat ctccgggaac gtggaatagt cagtcttttt
     1381 ctcggcggcg agttgatgct ccagccggga aattcggtca gaaagtgcgg cgatttccgg
     1441 gtcagtcgct ttctcctgcg acgcaacatg tgactgcata ccccagccac gctccggttg
     1501 atagagcgtc gcccagatcc acagaaacgg ccagataaca tgcagagtaa acaggctcac
     1561 ccagcccgcc gtatgaatag cgtcggcatg gggatggttg cgctttttgg caatcagata
     1621 agggatgtca tgtatcgcga tgattccata aaaaatcacc agaaagacga agatcagcac
     1681 tcccaacgcg aaatagttta gaaacatatc tgcctcggat tcacgtttat cagtgttgtt
     1741 tttgggctgg cagccagaag ggagtcaggc tgatattttg acaataatcc gggttcgcga
     1801 ttctcgccat aacaccaaag aataattttt agaggtgatg agttgcttag ttacataacg
     1861 attgtatgac gaaggcataa catgctgtag atcacatcag gtgaacgccg taagaaaata
     1921 tcttgtgatt cagatcacaa agattcaaca aaccatcaaa acaaaaatgt gacactactc
     1981 acatttaaat gccattttta gcgaaaatcg ccgccttgtt gcttttttac acaagcgttt
     2041 tgtgatgaac gtcacgtcaa ttacctctct accccctata tttatgtgat tgatatcaca
     2101 caaaaggccg tcgactggac agttaaccga ttcagtgcca gatttcgcag tatctacaag
     2161 gtccggctac ctctgccgcc acattaacaa aaaacctcgg gcttccagcc tgcgcgacag
     2221 caaacataag aaggggtgtt tttatgtcat ccgatattaa gatcaaagtg caaagctttg
     2281 gtcgtttcct cagcaacatg gtgatgccaa atatcggcgc gtttatcgcg tggggtatca
     2341 tcaccgcgtt atttattcca acagggtggt taccgaacga gacgctggcg aagctggtcg
     2401 ggccgatgat cacttatctc ctgccgctgc tgatcggtta taccggtggt aagctggtag
     2461 gcggcgaacg tggcggcgta gtcggtgcca tcaccaccat gggcgttatc gtcggcgcag
     2521 acatgccgat gttcctcggt tctatgattg caggtccgct gggcggctgg tgcattaagc
     2581 acttcgaccg ctgggtagac ggtaagatca aatccggttt tgagatgctg gtgaataact
     2641 tctccgcagg catcatcggg atgatcctcg ctattctggc attcctcggc attggcccga
     2701 ttgttgaagc cctgtccaaa atgctggctg cgggcgttaa cttcatggtt gtccatgaca
     2761 tgctgccgct ggcgtctatc tttgttgaac cggcgaaaat cctgttcctc aacaatgcca
     2821 ttaaccacgg tatcttctcg ccgctgggta ttcagcagtc ccatgaactg ggtaaatcaa
     2881 tcttcttcct gattgaagct aacccaggtc caggtatggg cgtgctgctg gcgtacatgt
     2941 tctttggtcg tggtagcgct aaacagtctg cgggcggtgc ggcaatcatc cacttcctgg
     3001 gtggtatcca cgaaatctac ttcccgtatg tgctgatgaa tccgcgtctg atcctcgcag
     3061 tcatcctcgg cggtatgact ggcgtgttca cgctgactat cctgggcggt ggtctggttt
     3121 ctccggcatc tccgggttct atccttgctg tactggcgat gacaccaaaa ggtgcttatt
     3181 tcgctaacat cgcgggtgtg tgtgcggcga tggctgtctc cttcgttgtc tctactattt
     3241 tgctgaaaac cagcaaagtg aaagaagaag atgatattga agcagcaact cgtcgtatgc
     3301 aggacatgaa agctgagtct aaaggcacat ctccgctgtc tgcgggcgat gtgactaacg
     3361 acctgagcca cgtacgtaaa atcatcgttg cctgtgacgc cggtatgggt tcaagtgcga
     3421 tgggcgcagg cgtgctgcgt aagaaaattc aggatgcagg tctgtcacag atttcggtca
     3481 ctaacagcgc gatcaacaac ctgccgccag atgtggacct cgtcatcact caccgtgacc
     3541 tgaccgaacg cgctatgcgc caggttccgc aggcgcagca tatttcgctg accaacttcc
     3601 tcgacagcgg cctgtacacc agcctaaccg aacgtctcgt tgctgcccag cgccatactg
     3661 aaaacgaagt gaaagtgaaa gacagtctga aagacagctt tgacgattcc agtgctaacc
     3721 tgttcaaact gggcgcggag aacatcttcc tcggtcgcaa agcagcaacc aaagaagaag
     3781 cgattcgttt tgctggcgag cagctggtga aaggcggtta cgttgagccg gaatacgttc
     3841 aggcgatgct ggatcgtgaa aaactgaccc cgacttatct gggtgagtct atcgcggtgc
     3901 cacacggtac ggttgaagcg aaagatcgcg tactgaaaac gggcgtggtg ttctgccagt
     3961 acccggaagg cgtgcgcttc ggtgaagaag aagatgacat tgcccgtctg gtgattggta
     4021 ttgcagcccg taacaacgag cacattcagg ttatcaccag cctgaccaat gcactggatg
     4081 atgagtccgt catcgagcgt ctggcacaca ccaccagcgt ggatgaagtg ctggaactgc
     4141 tggcaggtcg taagtaatcc aatcccaccc tctccacatg gagaaggtgg ggttaattgc
     4201 ctgatgcgct acgcttatca ggcctacagg atgcatcaca atttgttgaa tttgcacgtt
     4261 cttgtaggcc ggataaggcg cttacgccgc atccggcgct gcccctctcc tcacggagag
     4321 ggtttgggtg agggaaaagc ctcaccccag ccctctcggg taaaaacatt gatgaaggtt
     4381 aatactatga aagcattaca ttttggcgca ggtaatatcg gtcgtggctt tatcggtaaa
     4441 ctgctggcag acgcgggtat ccaactgacg tttgccgatg tcaatcaggt ggtacttgat
     4501 gccctgaatg cccgtcatag ctatcaggtt catgtggttg gtgaaaccga gcaggtagat
     4561 accgtttccg gcgtcaatgc tgtcagcagc attggtgatg atgtcgttga tctgattgct
     4621 caggtcgatt tagtcactac cgccgttggc ccggttgtgc tggaacgtat tgctccggct
     4681 atcgccaaag ggctggtgaa acgcaaagaa caaggtaatg aatccccgct gaacatcatc
     4741 gcctgtgaaa acatggtacg cggtaccacg cagctgaaag gccatgtgat gaacgccctg
     4801 ccggaagacg ccaaagcgtg ggtagaagaa cacgttggct ttgtcgattc cgccgttgac
     4861 cgcatcgtac cgccttcggc ttcggcaact aacgatccgc tggaagtgac ggtagaaact
     4921 ttcagcgaat ggattgtcga taaaacgcag ttcaaaggcg cactgccgaa catcccaggc
     4981 atggagttaa ccgacaacct gatggcattt gtcgaacgta aactcttcac cctgaacacg
     5041 ggtcatgcta taaccgcgta cctcggaaaa ctggccggtc atcagaccat tcgtgacgct
     5101 attctcgacg agaaaatccg cgcggtggta aaaggtgcga tggaagaaag tggtgcggta
     5161 ctgatcaagc gctacggctt tgacgcagac aaacatgcgg cgtacatcca gaaaatcctc
     5221 ggtcgttttg agaacccgta tctgaaagat gatgtagagc gcgtaggccg tcagccgctg
     5281 cgtaaactga gtgctggcga ccgtctgatc aagccactgc tcggtacgct ggaatacagc
     5341 cttccgcaca agaatctgat tcaggggatt gctggtgcaa tgcacttccg cagtgaagat
     5401 gatccgcagg ctcaggaact ggcagcactg atcgctgaca aaggtccgca ggcggcgctg
     5461 gcacagattt ccggtcttga tgccaacagc gaggttgtat ccgaggcggt aaccgcttat
     5521 aaagcaatgc aataatggtg gaccaggcgc aggacaccct gcgcccgaat aacagattgt
     5581 cagatatgca ggcaacaatg gaacaaaccc aggcctttga aaaccgtgtg cttgagcgtc
     5641 tgaatgctgg caaaaccgtg cgaagctttc tgatcaccgc cgtcgagctt ttgaccgagg
     5701 cggtaaatct tctggtgctt caggtattcc gcaaagacga ttacgcggtg aagtatgctg
     5761 tagaaccgtt actcgacggc gacggtccgc tgggcgatct ttctgtgcgt ttaaaactca
     5821 tttacgggtt gggcgtcatt aaccgccagg aatacgaaga tgcggagctg ctgatggcat
     5881 tgcgtgaaga actaaatcac gacggcaacg agtacgcctt taccgacgac gagatccttg
     5941 gaccctttgg tgaactgcac tgcgtggcgg cgttaccacc gccgccacag tttgaaccag
     6001 cagactccag tttgtatgca atgcaaattc agcgctatca acaggctgtg cgatcaacaa
     6061 tggtcctttc actgactgag ctgatttcca gaatcagctt aaaaaaagcc tttcaaaagt
     6121 aagcaacgtc tgcttactgc ccctctacct gcttcggccg ataaagccga cgataatact
     6181 ccagacgttg tagatataac ggcacgcttt catcgggtat tccggacgga atcgcgttac
     6241 ggggaggaag ttttttcaga tactcccgga acgcctggct tgatgccatg aaatctacgg
     6301 ctttatcgat aagaagcgga acgttttcac ctaatttgcc catgatgata tctccgtatt
     6361 accccgcgcc gggaatgcgc ggccgccaat tttagtttag gttcccggta aagatgacat
     6421 taaggaaaac gtgctgaatc ctcaataatc atccgctgct gttt
//
LOCUS       DAZKHF020000243         6451 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2201S31241
            SAMN43267800-rid25106413.denovo.245, whole genome shotgun sequence.
ACCESSION   DAZKHF020000243 DAZKHF020000000
VERSION     DAZKHF020000243.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267800
            Sequence Read Archive: SRR30307263
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6451)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6451)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 6451)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350581.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350581.2
            Genome Coverage       :: 151x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:21:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,652
            CDSs (total)                      :: 5,557
            Genes (coding)                    :: 5,320
            CDSs (with protein)               :: 5,320
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 237
            CDSs (without protein)            :: 237
            Pseudo Genes (ambiguous residues) :: 0 of 237
            Pseudo Genes (frameshifted)       :: 102 of 237
            Pseudo Genes (incomplete)         :: 130 of 237
            Pseudo Genes (internal stop)      :: 52 of 237
            Pseudo Genes (multiple problems)  :: 41 of 237
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6451
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267800-rid25106413.denovo.245"
                     /serotype="O 26:H11"
                     /isolate="2201S31241"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-01"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            <1..5746
                     /gene="efa1"
                     /locus_tag="ACHOSC_RS19775"
                     /old_locus_tag="pgaptmp_003955"
     CDS             <1..5746
                     /gene="efa1"
                     /locus_tag="ACHOSC_RS19775"
                     /old_locus_tag="pgaptmp_003955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001239084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="lymphostatin Efa1/LifA"
                     /protein_id="HGU4549793.1"
                     /translation="REGTLNSQNLRQLLENSVSSCFLTEQSINKLLSEAEKRPYQSLT
                     EIHQHLTGLPTIADATLSLLSVGLPGTGKLLRREQDYGRPPVTAIQDSTFVLPYNFKG
                     IGFNDNIISSAPVVSSLHFIAEHAKYTLLSWPEFYRHHAQRWFEMAKGYGSQNIDFHP
                     QSLLVTQEGRCMGLALLYLQTEDTAHYSILQENLMTVSALHQTSNRDKLPLSKDDNSL
                     MTRTYSLIEMLQYQGNKYITNESLLHKAAWNQERITLLFNEKGVKRALISTPNHTLVL
                     QQLEDIYRLTDPNFGHADFLSPIDALKFIEAMIQLTPTLQEYYGLLNKDINKHIQVHY
                     AESDMVWNKLLPENDAGLSTRIQHTTTDRLANLAEPVAVAGISLPVKTLYDIGATLDG
                     RRITSPPTSEQIPSLRLNGDVLNDYLSRTVLTPEQADNIRKILHTQGIRSGTRPIDPE
                     MIRGTQDDLVSSQTRLQRQATRVKQQLAGVLDTLQQHFQNIPRSSGRHLSVENIELAD
                     IGSGRFNLQIRDGETLHTTSVEVPEVVSRFQKLSTMLSALPASGIMDFDLGMSVVGVV
                     QYARLLQQGHEDSTLAKINLAMDIKQLSEATLGSMIQIAGNKFLNTEGIQGFRLESAV
                     AEGMRSVATRTGGTMGKALSASARVLELPVLETVLGTWNLYNSVIQLQQATSYSETMA
                     ARVQIAFDSISLGLTAASVAFPPLIIATGPIAAIGMGASSIARNVARKEERHTQWLEY
                     KKFLTDGSKHIVVASPERGLLDFSGNKVFGKMVLDLRQSPPLLHGESSFNADRKIGHR
                     PDLGDWQIREKVGYANSISPYSSLAHGYANSKWPQTIPKIPSGEYDTIILGYGHQYQA
                     NTEIEYLSNWIVWREAVPDSTSRHKRPPLEVLNSQCTVIAGERKTTVLPLRVLSDLTP
                     ECTEQAISLKDYKFILRGGSGGLAVQVGGAGYYDIDANPVAKENTLSFRGLPEEFPLA
                     FDLSKQTQSVMLKTTDGEVPVMTITQKGINTLVGTTAGENWLTGNDKDNTFHTSSGGG
                     TVISGGGNNRYIIPRDLKTPLTLTLSNSSVSHEILLPETTLTELKPVAFELSLIYWAG
                     NNIKVQLEDETQLNRFAGNFRGHTSDGITLEAVSQENGIQLGVSSCDVQRWQAVYPEE
                     NNRPDAILDRLHDMGWSLAPEVRFRGGETSASYDPLNRQLVYRLQERYSEFRLTGSRH
                     YTTAVTGTPGSRYIITGPDTAQISPAQIILAGDNDHPETIDLLEASPVLVEGKKDKNS
                     VILTIATIQYSLQLTISGIDESLPGTTRVAIQPQDTRLLGDVLRILPDNGNWVGIFRS
                     GHTPTVNRLENLMALNQVMTFLPRVSGSAEQVLCLENLGGVRKKVEGELLSGKLKGAW
                     KAEGEPTVPVNISDLSIPPYSRLYLIFEGKNNVLLRSKVHAAPLKITSAGEMQLSERQ
                     WQQQEHIIVKPDNEAPSLILSEFRRFTISSDKKFSLKLMCHQGMVRIDRRSLSVRLFY
                     LREQPGIGSLRLTFRDFFTEVMDTTDREILEKELRPILIGDTHRFINAAYKNHLNIQL
                     GDGVLNLADIVAEYARIQKEETSKILYQYQGAMKKKTDGPSVVEDAIMTTTVTTDSGE
                     PFPTFHPWYTDDLSGRYKSVPMARKADTLYHLTPKGDLQIIYQVATKMVNQAMIVSLP
                     NYRHEWEKYNLSILSEIPQNNNTVVHSILRVNGPTMQVRTIDYRGTDENNPIVSFSDT
                     TFINGEQMLSYDSHSSGRVYSREEYMMWELQQRVSEASSARTQDYWLMDAAVRNGEWK
                     ITPELLRHTPGYIRSTVSKWSRGWLKTGTILQTPEDRNTDVYLTTIQNNVFSRQGGGY
                     QVYYRIDGMAGADIADNAPGETRCTLRPGTCFEVTSVDERHYEWNIIYVTLKTCGWSR
                     NGQSKTPNGDNLFN"
     gene            5918..6268
                     /locus_tag="ACHOSC_RS19780"
                     /old_locus_tag="pgaptmp_003956"
                     /pseudo
     CDS             5918..6268
                     /locus_tag="ACHOSC_RS19780"
                     /old_locus_tag="pgaptmp_003956"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015869530.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
BASE COUNT         1888 a         1481 c         1510 g         1572 t
ORIGIN      
        1 gcgagaaggt acactaaatt cacagaatct tcgtcagctt ctggaaaact ctgtttcttc
       61 atgctttttg actgagcaaa gtattaataa attacttagt gaggcagaaa aaaggcctta
      121 tcagtcttta acagaaatac atcagcatct cacaggatta ccaactattg ccgatgcaac
      181 cctttcatta ctttctgttg gattacctgg tacgggaaaa ctattgcgca gggagcagga
      241 ctatgggcgt ccaccagtta cagcaattca ggattccaca tttgtactcc cttataattt
      301 caaaggtatt ggttttaacg ataacattat atcctctgca cctgtagtct cctcattaca
      361 ttttatcgct gaacatgcga aatatacttt gttgtcatgg cctgagtttt atcgtcatca
      421 tgcacagcga tggttcgaaa tggctaaagg atatggaagc cagaatattg attttcaccc
      481 tcagtctcta ttggtaaccc aagaaggacg ctgtatggga ttagccttac tttatttaca
      541 gactgaagat actgctcatt atagcattct ccaggaaaac ctaatgactg tgagtgcact
      601 tcatcagacc agtaatcgcg ataagttgcc actgtccaaa gatgataatt ccttaatgac
      661 aagaacttat agtctgattg aaatgctaca gtatcaggga aacaaatata ttaccaacga
      721 atcgctacta cataaggccg catggaacca agaaagaata actttattat tcaatgaaaa
      781 aggagttaag cgagccctga taagcacgcc taatcatact ctggttctgc aacaactgga
      841 ggatatttac cggctcactg atccaaattt tgggcatgca gatttccttt cacctataga
      901 tgctctgaag tttattgagg ctatgataca attaactcca acacttcagg aatattatgg
      961 cctattaaac aaagacatta ataaacatat acaagtacat tatgccgaat cagatatggt
     1021 ctggaataag cttctgccag aaaatgatgc tggactgagc accagaattc agcacaccac
     1081 caccgaccgt ctggcgaatc tggctgaacc agtcgctgtt gcaggtatct ccctgccagt
     1141 aaaaacactt tatgatatcg gagccaccct tgacggtcgg cgcatcacct ctcctccaac
     1201 atcggagcaa atcccttctc tgcgtctcaa cggtgatgtt ctgaatgatt atctgtcccg
     1261 cacagttctg actccagaac aggctgataa cataagaaaa atactgcaca ctcagggaat
     1321 acgcagcggt acccgtccca tagatccgga gatgattcgt gggacgcagg atgacctagt
     1381 ttcgtcacag actcgtctgc aaaggcaagc aacacgggtt aaacagcaac tcgccggcgt
     1441 acttgatact ctgcaacagc acttccagaa cattccacgt tcatccggtc gtcatctttc
     1501 tgtagagaat attgagctgg ctgatatcgg aagtgggcgt ttcaaccttc aaattcgaga
     1561 tggagaaaca ttgcatacca cttctgtgga agtaccggaa gttgtgtccc gttttcagaa
     1621 actttctacc atgctttcag ccctgcctgc cagtggaatc atggatttcg acctcggcat
     1681 gagtgtggtc ggagtcgtcc agtatgcccg cctgctacag caggggcacg aagacagtac
     1741 cctagccaag ataaatctag ctatggatat caagcaactt tccgaagcaa ctctcggcag
     1801 catgattcag attgccggga ataagtttct caatacagaa ggaatccagg ggttcagact
     1861 ggaaagcgcc gtcgctgaag gtatgcgctc agtagcaacc cgtaccggag gcacaatggg
     1921 gaaagccctt tctgccagtg cccgtgttct tgagctgcct gtactggaaa cagttctggg
     1981 gacatggaac ctgtacaaca gcgtcattca gctccagcaa gctacatctt attctgagac
     2041 aatggctgcc cgggtacaga ttgcgtttga ttccatttct ctgggattaa ctgccgcttc
     2101 ggtagctttc ccgccactaa ttattgccac tggccccatt gcggctattg gtatgggagc
     2161 ttccagtatt gcacgtaatg tggcacggaa agaagaacgg catacacaat ggctggaata
     2221 taaaaaattc ctgactgatg gcagtaaaca cattgttgtg gcctctccgg aaagaggtct
     2281 gctggatttc tccggaaaca aagtttttgg aaaaatggtg ctggatctgc gtcagtctcc
     2341 tcctctcttg catggagaaa gctcttttaa cgctgaccgc aaaatcggtc atcgtccgga
     2401 tctgggggac tggcaaattc gtgagaaggt ggggtatgcc aacagtatca gtccctactc
     2461 ttccctggcg cacggttacg ccaacagtaa atggccacaa acaataccga aaattccctc
     2521 gggagaatat gacaccataa ttctgggcta cggtcaccag tatcaggcca atacggaaat
     2581 agaatatctg tctaactgga ttgtatggcg ggaagccgta ccagacagta cttcccgcca
     2641 caaacgtcct cctctggagg ttcttaatag tcagtgtact gtgatagctg gagagcgtaa
     2701 aaccacagta cttcccctga gagtgctcag cgatctgaca ccggaatgca cagaacaggc
     2761 tatatcgtta aaagattata aattcatact gagaggggga agcggtgggc tggctgttca
     2821 ggtcggtggc gcgggatatt atgatattga tgcaaatcct gtggcaaaag aaaatacgct
     2881 ctcttttcgc gggctaccgg aagagtttcc gctcgccttt gatttatcaa aacaaacaca
     2941 gtcggtcatg ctgaaaacaa cggacggtga ggtgccggta atgaccatca cccagaaggg
     3001 aataaacacc ctggtcggta caacggccgg cgaaaactgg ctaacgggta acgataagga
     3061 caataccttc catacgagtt ctggtggcgg tactgtcatc tctggtggag ggaataaccg
     3121 ctatattatc ccccgcgatt taaaaactcc gctgacactg acgctgtcta atagctcggt
     3181 ctctcacgag atccttctgc cagaaacaac tctgacagaa ttaaaacctg tcgcctttga
     3241 gctgagtctg atttactggg ccgggaacaa tataaaggtt cagctggagg atgagacaca
     3301 actgaaccgt tttgccggaa acttcagggg gcataccagt gatggcatta ctctggaggc
     3361 ggtttcccag gaaaatggta ttcaactggg ggtttcatca tgtgatgttc aacgctggca
     3421 ggctgtttat ccggaagaaa acaacagacc agacgccata ctggacaggc tgcatgatat
     3481 gggatggagc ctggcccccg aggtcaggtt ccggggagga gaaacatctg ccagctatga
     3541 ccccctgaac cgtcagctcg tttaccggct tcaggagcgt tactcggaat tcaggctgac
     3601 cggcagtcgc cattacacca cggctgtaac cggaaccccc ggaagccggt acattattac
     3661 ggggccggat acagcacaga tatcaccggc acaaatcata ctggccggtg ataatgacca
     3721 tccggaaacg attgatttgc ttgaggccag tcctgttctg gttgaaggga aaaaagacaa
     3781 aaacagcgtg atattaacga ttgctacgat tcagtattcc cttcaactga caatatccgg
     3841 gatcgacgaa tcgctgcccg ggacaacccg tgtggcaatt cagcctcagg atacccgttt
     3901 actgggtgac gtactccgga tcttaccaga taatggtaac tgggtgggga ttttccggag
     3961 tggtcataca ccaacggtaa accggctgga aaatttgatg gcactgaatc aggtaatgac
     4021 gttcctgccc cgggtatccg gaagtgcaga gcaggtatta tgcctcgaaa acctaggtgg
     4081 cgtaaggaaa aaagtggagg gggagttact gtcagggaag ctgaaaggtg cgtggaaagc
     4141 cgaaggtgaa cctactgttc cggtaaatat ctcagatcta agtatcccac cctattcacg
     4201 tctgtatctg atttttgaag ggaaaaataa tgtgttgcta cgcagtaaag tacatgcagc
     4261 tccgttgaaa ataacatccg caggagagat gcagttatct gaaaggcagt ggcaacagca
     4321 ggaacatatt attgtcaagc ccgacaacga agccccctca ttaatactca gtgaatttcg
     4381 tcgtttcact atttcatcgg ataaaaaatt ttctttaaaa ctgatgtgcc atcaggggat
     4441 ggtccgcatc gaccgcagat cattatcggt cagattgttc tatctgcgtg aacagccagg
     4501 tatcggcagt ttacgtctga cgttcagaga ttttttcaca gaagtgatgg atacaacgga
     4561 cagggaaatt ctggagaaag agctgagacc aattctgata ggagatacac accgctttat
     4621 caacgctgca tacaaaaatc atctgaatat ccagttagga gatggcgttc tgaatctggc
     4681 agacattgtt gcggaatacg cccgtattca aaaggaagaa acatcaaaaa tattgtatca
     4741 atatcaaggt gccatgaaaa aaaaaacaga tggaccatct gtggtagaag atgccattat
     4801 gaccactact gtcacaacag attcaggtga accattccct accttccacc cgtggtatac
     4861 agatgattta tcagggcgtt ataagagcgt acctatggca agaaaagcag atactttgta
     4921 tcacctgaca ccaaaaggtg atctacagat aatatatcag gtagctacaa aaatggtgaa
     4981 tcaggcgatg attgtatccc tgccaaacta ccgacacgag tgggaaaaat ataatttaag
     5041 catcttatcc gaaatccctc agaacaataa tactgttgta cattcaatcc tcagggttaa
     5101 tggccccaca atgcaggtgc gcacaattga ctacagagga acggatgaaa acaatcccat
     5161 agtatctttt tcagatacaa ccttcatcaa tggtgaacag atgttgagtt atgactcgca
     5221 ttcatcaggg cgagtctatt ccagagaaga atatatgatg tgggaattgc agcaacgggt
     5281 atcagaagct tccagtgccc ggacacagga ttactggctg atggatgcag cggtaagaaa
     5341 cggagaatgg aagatcacac cagaattatt acgtcacaca ccgggatata tccggagtac
     5401 ggtatcgaaa tggtccagag gatggctgaa aaccggcaca atactccaga ctccagaaga
     5461 cagaaatacg gatgtatacc tgactaccat acagaacaat gtatttagtc gtcagggggg
     5521 cggctaccaa gtgtattatc ggattgatgg aatggctggt gcggatatag cggataatgc
     5581 accaggggaa acccgctgca ccctcaggcc cggaacatgt tttgaagtga caagtgtgga
     5641 tgaaaggcat tatgagtgga atatcattta tgtcacgctg aaaacctgtg gctggagccg
     5701 aaatggccaa agcaaaacgc cgaatggtga caaccttttt aactaattaa aatctctgtt
     5761 aaagatgtat aacccggcat ctatgtgctc accagcatat agatgcaatg tgattcccct
     5821 gatggtcgta tcagagatgg tatgcgagcc tgtacataga tttgtgtaat tgcctgattt
     5881 tgatatgttc aatccaacat caaaagcagg ttaatttatg gacgaaaaac agttgcaggc
     5941 tctggctaac gaacaggcca aaaatctcaa aacccctgaa gatctcagcc agttcgatcg
     6001 cctgctgaag aaaatcagcg ttgaggcagc tctcaacgct gaaatgtccc accatctggg
     6061 ctacgataaa aaccagccta aaccgggtgc caactcccgc aatggctatt ccacaaagac
     6121 cgttatcacc ggtgatggcc ccctggaact acgcactccg cgcgatcgtg atggctcttt
     6181 tgaaccgtaa cttgtgaaga aaaaccagac ccggatcacc gggatggata accagatcct
     6241 gtcgttgtac gccaaaggcc tgacaaccgt aagcgtacag cctgaaccgt ctggtcagaa
     6301 tctgacgaat tagagtaagc tggacatccc cagatatatt atggctgcca gcgtcatatc
     6361 ggcataaata gagatatccc ttaacgctaa ccagaaaaaa atacctataa gagcctgtac
     6421 atagagttgt gtcgttgcct gatgttgata t
//
LOCUS       DAZKHF020000244         6409 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2201S31241
            SAMN43267800-rid25106413.denovo.246, whole genome shotgun sequence.
ACCESSION   DAZKHF020000244 DAZKHF020000000
VERSION     DAZKHF020000244.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267800
            Sequence Read Archive: SRR30307263
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6409)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6409)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 6409)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350581.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350581.2
            Genome Coverage       :: 151x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:21:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,652
            CDSs (total)                      :: 5,557
            Genes (coding)                    :: 5,320
            CDSs (with protein)               :: 5,320
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 237
            CDSs (without protein)            :: 237
            Pseudo Genes (ambiguous residues) :: 0 of 237
            Pseudo Genes (frameshifted)       :: 102 of 237
            Pseudo Genes (incomplete)         :: 130 of 237
            Pseudo Genes (internal stop)      :: 52 of 237
            Pseudo Genes (multiple problems)  :: 41 of 237
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6409
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267800-rid25106413.denovo.246"
                     /serotype="O 26:H11"
                     /isolate="2201S31241"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-01"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            complement(<1..136)
                     /locus_tag="ACHOSC_RS19785"
                     /old_locus_tag="pgaptmp_003957"
     CDS             complement(<1..136)
                     /locus_tag="ACHOSC_RS19785"
                     /old_locus_tag="pgaptmp_003957"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4549794.1"
                     /translation="MLQSSADKACNVLVKKVRYLKDVRKITKMMNGKLIYSWLKWPCG
                     E"
     gene            214..1308
                     /gene="ddlA"
                     /locus_tag="ACHOSC_RS19790"
                     /old_locus_tag="pgaptmp_003958"
     CDS             214..1308
                     /gene="ddlA"
                     /locus_tag="ACHOSC_RS19790"
                     /old_locus_tag="pgaptmp_003958"
                     /EC_number="6.3.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008716 - D-alanine-D-alanine ligase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--D-alanine ligase"
                     /protein_id="HGU4549795.1"
                     /translation="MEKLRVGIVFGGKSAEHEVSLQSAKNIVDAIDKSRFDVVLLGID
                     KQGQWHVSDASNYLLNADDPAHIALRPSATSLAQVPGKHEHQLIDAQNGQPLPTVDVI
                     FPIVHGTLGEDGSLQGMLRVANLPFVGSDVLASAACMDKDVTKRLLRDAGLNIAPFIT
                     LTRANRHNISFAEVESKLGLPLFVKPANQGSSVGVSKVTSEEQYAIAVDLAFEFDHKV
                     IVEQGIKGREIECAVLGNDNPQASTCGEIVLTSDFYAYDTKYIDEDGAKVVVPAAIAP
                     EINDKIRAIAVQAYQTLGCAGMARVDVFLTPENEVVINEINTLPGFTNISMYPKLWQA
                     SGLGYTDLITRLIELALERHAADNALKTTM"
     gene            complement(1332..1544)
                     /gene="yaiZ"
                     /locus_tag="ACHOSC_RS19795"
                     /old_locus_tag="pgaptmp_003959"
     CDS             complement(1332..1544)
                     /gene="yaiZ"
                     /locus_tag="ACHOSC_RS19795"
                     /old_locus_tag="pgaptmp_003959"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414914.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2754 domain-containing protein"
                     /protein_id="HGU4549796.1"
                     /translation="MNLPVKIRRDWHYYAFAIGLIFILNGVVGLLGFEAKGWQTYAVG
                     LVTWVISFWLAGLIIRRRDEETENAQ"
     gene            1804..2112
                     /gene="yaiY"
                     /locus_tag="ACHOSC_RS19800"
                     /old_locus_tag="pgaptmp_003960"
     CDS             1804..2112
                     /gene="yaiY"
                     /locus_tag="ACHOSC_RS19800"
                     /old_locus_tag="pgaptmp_003960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YaiY family protein"
                     /protein_id="HGU4549797.1"
                     /translation="MADFTLSKSLFSGKYRNASSTPGNIAYALFVLFCFWAGAQLLNL
                     LVHAPGVYERLMQVQETGRPRVEIGLGVGTIFGLIPFLVGCLIFAVVALWLHWRHRRQ
                     "
     gene            complement(2171..3265)
                     /gene="yaiW"
                     /locus_tag="ACHOSC_RS19805"
                     /old_locus_tag="pgaptmp_003961"
     CDS             complement(2171..3265)
                     /gene="yaiW"
                     /locus_tag="ACHOSC_RS19805"
                     /old_locus_tag="pgaptmp_003961"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="surface-exposed outer membrane lipoprotein YaiW"
                     /protein_id="HGU4549798.1"
                     /translation="MSRVNHLSSLSLLAVLVLAGCSSQAPQPLKKGEKAIDVASVVRQ
                     KMPASVKDRDAWAKDLATTFESQGLAPTLENVCSVLAVAQQESNYQADPAVPGLSKIA
                     WQEIDRRAERMHIPAFLVHTALKIKSPNGKSYSERLDSVRTEKQLSAIFDDLISMVPM
                     GQTLFGSLNPVRTGGPMQVSIAFAEQHTKGYPWKMDGTVRQEVFSRRGGLWFGTYHLL
                     NYPASYSAPIYRFADFNAGWYASRNAAFQNAVSKASGVKLALDGDLIRYDSKEPGKTE
                     LATRKLAGKLGMSDSEIRRQLEKGDSFSFEETALYKKVYQLAEAKTGKSLPREMLPGI
                     QLESPKITRNLTTAWFAKRVDERRARCMKQ"
     gene            complement(3278..4498)
                     /gene="sbmA"
                     /locus_tag="ACHOSC_RS19810"
                     /old_locus_tag="pgaptmp_003962"
                     /pseudo
     CDS             complement(3278..4498)
                     /gene="sbmA"
                     /locus_tag="ACHOSC_RS19810"
                     /old_locus_tag="pgaptmp_003962"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706191.2"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:1904680 - peptide transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015833 - peptide transport [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="peptide antibiotic transporter SbmA"
     gene            4850..6007
                     /gene="ampH"
                     /locus_tag="ACHOSC_RS19815"
                     /old_locus_tag="pgaptmp_003963"
     CDS             4850..6007
                     /gene="ampH"
                     /locus_tag="ACHOSC_RS19815"
                     /old_locus_tag="pgaptmp_003963"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine-
                     carboxypeptidase/endopeptidase AmpH"
                     /protein_id="HGU4549799.1"
                     /translation="MKRSLLFSAVLCAASLTSVHAAQPITEPEFASDIVDRYADHIFY
                     GSGATGMALVVIDGNQRVFRSYGETRPGNNVRPQLDSVVRIASLTKLMTSEMLVKLLD
                     QGTVKLNDPLSKYAPPGARVPTYNGTPITLVNLATHTSALPREQPGGAAHRPVFVWPT
                     REQRWKYLSTAKLKAAPGSQAAYSNLAFDLLADALANASGKPYTQLFEEQITRPLGMK
                     DTTYTPSPDQCRRLMVAERGASPCNNTLAAIGSGGVYSTPGDMMRWMQQYLSSDFYQR
                     SNQADRMQTLIYQRAQFTKVIGMDVPGKADALGLGWVYMVPKEGRPGIIQKTGGGGGF
                     ITYMAMIPQKNIGAFVVVTRSPLTRFKNMSDGINDLVTELSGNKPLVIPAS"
     gene            complement(6008..>6409)
                     /locus_tag="ACHOSC_RS19820"
                     /old_locus_tag="pgaptmp_003964"
     CDS             complement(6008..>6409)
                     /locus_tag="ACHOSC_RS19820"
                     /old_locus_tag="pgaptmp_003964"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414909.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HGU4549800.1"
                     /translation="DGLMKNDIPYKLISEGNCTGYHLPAKQTITLIEQNQLWRDAFYW
                     LAWQNRILELRDVQLIGHNSYEQIRATLLSMIDWNEELRSRIGVMNYIHQRTRISRSV
                     VAEVLAALRKGGYIEMNKGKLVAINRLPSEY"
BASE COUNT         1603 a         1650 c         1570 g         1586 t
ORIGIN      
        1 actcaccgca tggccattta agccaggaat aaattagctt cccgttcatc atttttgtga
       61 tcttgcgcac atctttgaga taacgcactt tcttcaccag tacgttgcac gctttgtcag
      121 cagaagattg caacaatctt tcagaaggcc gcgaatcccc cgttttgtcg tggtaatgta
      181 tgcactttgt gttagacggg ataggttttt aagatggaaa aactgcgggt aggaatcgtt
      241 tttggtggta aatcagcgga acatgaagtg tctctgcaat cggcaaaaaa cattgtcgat
      301 gccattgata aaagtcgctt cgacgttgtg ctgctgggca ttgataaaca agggcaatgg
      361 cacgtcagcg atgccagcaa ttatctgcta aatgcagacg atcctgccca tattgcgtta
      421 cgcccttcgg cgaccagcct tgcgcaggtg ccaggtaaac atgagcatca gcttatcgac
      481 gcgcaaaacg gtcagccgtt gccgacggtg gatgtcattt tcccgattgt ccacggtacg
      541 ctgggcgaag atggttcctt gcagggaatg ctgcgggtcg ccaatttacc gttcgtaggt
      601 tctgatgttc tggcttcagc agcctgtatg gataaagatg ttaccaaacg tctgctgcgc
      661 gatgccgggc tgaacattgc gccatttatt accctgacgc gcgccaatcg tcacaacatt
      721 agttttgccg aagtggagtc taaactgggg ttaccactgt ttgtaaaacc ggctaatcag
      781 ggctcttctg ttggtgtcag caaagtaacc agtgaagaac agtacgcaat tgccgtcgat
      841 ctggcgttcg aattcgatca taaagtgatc gttgagcaag ggatcaaagg tcgtgagatc
      901 gaatgcgcag ttctgggcaa cgacaacccg caagccagca cctgtggcga gatcgtactc
      961 accagcgact tctatgccta cgacaccaag tacattgacg aagatggcgc gaaagtggta
     1021 gttccggcag ccattgcgcc agaaatcaac gataagatcc gggcgattgc cgttcaggct
     1081 tatcaaacgt tgggatgcgc aggcatggcg cgtgtagacg tgtttttaac cccagaaaac
     1141 gaagtggtga tcaacgagat caacacactg cctggcttca ccaacatcag tatgtatccg
     1201 aagctgtggc aagccagcgg tctgggttac accgatctga tcacacgttt gattgagctg
     1261 gcgctggagc gtcacgctgc ggataacgca ttgaaaacca caatgtaata ttttaacgcc
     1321 cgataatatt cttattgggc gttttcagtt tcttcatcgc gacgacgaat aatcagcccc
     1381 gccagccaga aactaatcac ccacgtcacc agaccgacgg cataggtctg ccaacctttt
     1441 gcttcaaatc ccagtaaccc caccacgcca ttaagaatga atataaggcc aatggcgaac
     1501 gcatagtagt gccagtcacg gcggattttt acaggcagat tcatggcagc tccgttaacg
     1561 aaaaacaaca tccttgcata ttctgtaaca accgtctgtg gtgtagacaa cattttcgta
     1621 attgttaata aatttaactt aaggttgcca ttttgtgact cacaacgaag attcacgctt
     1681 cgggaatatt cccaacatca atttaccatg attctgatat tgacaatcta aatgacctgt
     1741 cagacttgcc ataagccgct ggtatcacac cagaagagaa gcagatgact tccggaggtt
     1801 tttatggctg atttcaccct gtcaaaatcg ctgtttagcg gaaaatatcg caatgcctct
     1861 tcaacgcctg gcaacattgc ctatgcgttg tttgtgctgt tttgcttttg ggctggggcg
     1921 caattgctga acctgttagt gcatgcgccc ggcgtctatg agcgtttaat gcaggtccag
     1981 gaaacaggtc gcccacgggt ggaaattggt ttaggtgtcg gcaccatttt cgggctgatc
     2041 ccgtttttag taggctgcct catttttgca gtggtggcgc tatggctgca ctggcgacat
     2101 cgccgccagt aagatttacg ttgccagata cggcctaaaa cgccttatct ggcatgacat
     2161 tcctgtttga tcactgtttc atacaacgcg cccgccgttc gtctacgcgc ttcgcaaacc
     2221 aggccgtagt caggttgcgg gtgattttcg ggctttccag ttgaatgcca ggcaacattt
     2281 cgcggggtag tgatttaccc gttttcgctt cggcaagttg ataaactttc ttgtacagcg
     2341 ccgtctcctc aaaagagaag ctgtcacctt tctctaactg acggcgaatt tcgctgtcgc
     2401 tcatccccag ttttcccgcc agtttgcgcg ttgccagttc ggttttcccg ggttctttac
     2461 tgtcgtaacg aattaaatca ccgtccagtg ccagcttcac gccgctggcc ttactgactg
     2521 cgttctgaaa cgcggcatta cggctggcgt accagccagc gttaaaatca gcaaagcggt
     2581 atatcggtgc gctatagctg gcgggataat tcagtaaatg gtaagtacca aaccacaacc
     2641 cgccgcggcg actgaagact tcctgacgaa ctgtaccgtc cattttccac ggataccctt
     2701 tggtatgctg ttcggcaaaa gcaatgctga cctgcatcgg cccaccggtg cgcacaggat
     2761 taagcgaacc aaacagcgtc tgccccattg gtaccatgct gatcagatcg tcaaaaatcg
     2821 cgctcaattg cttttctgta cgcaccgaat ctaaccgttc gctataactt ttgccgtttg
     2881 gcgatttgat ttttagcgcg gtatgaacca gaaaagcagg aatgtgcatc cgttcagcac
     2941 gacggtcaat ctcttgccag gcgatcttgc ttaagcccgg aacggccgga tctgcctgat
     3001 aatttgactc ctgctgcgcc accgccagca ccgagcagac gttttccagc gtcggtgcca
     3061 gcccctgact ttcaaaggtg gtggcgagat ctttcgccca ggcatcgcgg tctttcacgc
     3121 tcgcaggcat cttctgccgc acgacgctcg ctacatctat cgctttttcg ccctttttca
     3181 acggttgcgg agcctgacta ctacaccccg ctaaaaccag aaccgccaac aatgacagcg
     3241 aagatagatg attgacgcgc gacattactt ctccttttta gctcaaggta tgggttactt
     3301 cctgaatttt gtcaccatcc agctcatgtt caaagctgcg cagacgtttg tagatagaca
     3361 tcaactcaac cagtgtggtc catgagttaa tcaggtactg gaaagcaccg cgaacctgac
     3421 caaaaacgtt ggtaatctgc gtcatcaggc cgagcgtaat cgtaccggca acaattgacg
     3481 gaaacagcaa gaacaaaccg aaaacgttat cgacctgcaa atagagaatg cgggcgatgt
     3541 tgaaatacat atagtgaaaa tagaggcgaa aatagttttt ccgtacggcg ctaaacagct
     3601 cgcgtaccgt aggcggcgtc gcgcgcgtgg catcgtcttc accataaacc agctctttac
     3661 ggtaggcggc ctctactcgc tggtttttaa actccagccc cggcagtttg atccctacca
     3721 ctgccagcaa tccggtcccc atcagcgacc agacgatagc ggcaatcacc agaccatacg
     3781 gaatgtgccc gataatcggc agctccggca catgcgcgga gagcgttacc agcaccggca
     3841 gaaaggcgat caacgtcatg atggcgttga taaaactgac gcccatattc tccagcgttg
     3901 aagcaaaacg catggtgtct tcctgcacac gctgtgcggc accttcgata tgacgcagtt
     3961 gttgccagtt cgccatgtaa tattcgttca tcgccgtacg ccagcggaac acatagtgac
     4021 tgacaaagaa gttgttcagt acactgatca ccaccgcgat cagcgcaatc cccagaaaga
     4081 cgcccacttc gcggtaaaat tgttcgatag tgactttatg cggcgaactt agcgcggttt
     4141 gaatcagatc atagaacggt gcatactagg cgttgacggc gaccccgact tccaccaaaa
     4201 accaggtgac gaagatgatc agtgcagtac cgagtattga ccagtattgc caacggtgcg
     4261 ggctgtagat aaaccagaaa aatgcaaaaa gtcctacgca aacaatgtag taagcgtaaa
     4321 aaatcaggaa atccaacgac cagaaacgcg cggcgctaat cgggatctgc ccggaagcgc
     4381 cggtgatacg cgccacccag tcacccccac cggcttgcca gaagataacg gcaatcaatg
     4441 cccaaacaaa ggccgagaga aaaaacgttc ccggctttgg gaaaaaagac ttaaacatat
     4501 gcactcctgc taacttctta tcgttttaac aactgctgtg tattaaccgc atgaccgcta
     4561 caaagcgtca gccaaatgaa accgcccatt gtgacccata gcaaggcgct tagttcgcca
     4621 gcctgaccat aagaattgtt tcgacggatt tcacaaacca aagattgttt acataagcgg
     4681 attaacacgc gcaggatcac aaatgcaggc attggtgagc aggaaaaaga tcgtgtttac
     4741 gcgccgttac gtttttaaca ctattattgc actaattcgc cctttgcaat ccatcaatga
     4801 gtagtataaa tacgctcagt taccttcatt caatctatgg acaccaccgt tgaaacgtag
     4861 tctgcttttt tctgccgtgc tgtgtgcggc gtcattgacc tccgtccatg cggcgcagcc
     4921 gatcactgaa ccggagtttg cctctgatat tgtcgatcgt tatgccgatc atatttttta
     4981 cggcagcggt gccacgggga tggcgctggt tgttatcgac ggtaaccagc gcgtctttcg
     5041 cagttatggc gaaacgcgac ctggtaataa cgttcgcccg cagctggatt cggtcgtgcg
     5101 tattgcttcc ctcaccaagc taatgaccag tgaaatgctg gtgaaattgc tcgaccaggg
     5161 gaccgtgaag ttaaacgatc cgctaagtaa atatgctccg ccaggcgcac gcgtgccaac
     5221 ctacaatggg acgcccatta cgctggtcaa tctggcaacc cataccagcg cgctgccccg
     5281 tgaacagccc ggtggcgcgg cacatcgtcc ggtgtttgtc tggccaacgc gcgagcaacg
     5341 ctggaaatac ctttctacgg cgaagctgaa agccgcgcca ggcagtcagg cggcgtactc
     5401 taaccttgcg ttcgacctgc tggctgatgc actggcgaat gcgtccggca agccttatac
     5461 ccagttgttt gaagagcaga ttacccgtcc attggggatg aaagacacca cctacacccc
     5521 ctcaccggat cagtgccgcc gtttaatggt tgcagagcgc ggtgccagtc catgcaataa
     5581 cacgctggcg gcaattggca gcggcggcgt ctattccacg cctggcgata tgatgcgctg
     5641 gatgcagcag tatttgtcat ctgattttta tcagcgcagt aaccaggcag atcgcatgca
     5701 gacgttgatt tatcagcgcg cgcagtttac gaaagtgatt ggcatggatg tccccggcaa
     5761 agcggatgcg cttggtttag gctgggtata tatggtcccg aaagagggtc gtccggggat
     5821 tattcagaag acaggaggtg gtggcggttt cattacctat atggcgatga tcccgcagaa
     5881 gaatattggc gcgtttgtgg ttgtcactcg ttcgccgttg acgcgcttta aaaatatgag
     5941 cgatggcatc aatgacctgg tgaccgagct aagcgggaat aaaccgctgg ttatccccgc
     6001 gtcctgatta atactctgaa ggcaaacggt tgatagcgac cagtttgcct ttattcattt
     6061 cgatatagcc gcctttacgc aaagcagcga gaacttctgc gacgacagaa cgcgatatgc
     6121 gtgtacgttg atggatataa ttcatcacac caatacgtga tcgcaattct tcattccagt
     6181 caatcattga taataatgtt gcgcggattt gttcgtagga attatgccca atgagctgca
     6241 cgtcgcgtaa ttccagaatt ctattttgcc aggctaacca gtaaaaagcg tctcgccaga
     6301 gttgattttg ttcaataagc gtaatggttt gtttggctgg tagatgatat cccgtacaat
     6361 ttccttctga tattaatttg tatggtatat cgtttttcat taaaccatc
//
LOCUS       DAZKHF020000245         6377 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2201S31241
            SAMN43267800-rid25106413.denovo.247, whole genome shotgun sequence.
ACCESSION   DAZKHF020000245 DAZKHF020000000
VERSION     DAZKHF020000245.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267800
            Sequence Read Archive: SRR30307263
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6377)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6377)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 6377)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350581.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350581.2
            Genome Coverage       :: 151x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:21:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,652
            CDSs (total)                      :: 5,557
            Genes (coding)                    :: 5,320
            CDSs (with protein)               :: 5,320
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 237
            CDSs (without protein)            :: 237
            Pseudo Genes (ambiguous residues) :: 0 of 237
            Pseudo Genes (frameshifted)       :: 102 of 237
            Pseudo Genes (incomplete)         :: 130 of 237
            Pseudo Genes (internal stop)      :: 52 of 237
            Pseudo Genes (multiple problems)  :: 41 of 237
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6377
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267800-rid25106413.denovo.247"
                     /serotype="O 26:H11"
                     /isolate="2201S31241"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-01"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            <1..239
                     /locus_tag="ACHOSC_RS19825"
                     /old_locus_tag="pgaptmp_003965"
     CDS             <1..239
                     /locus_tag="ACHOSC_RS19825"
                     /old_locus_tag="pgaptmp_003965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308874.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="peptidase M85"
                     /protein_id="HGU4549801.1"
                     /translation="HEKNERAFFERLGTISDRYEASPDFTEYSAVSNIGYGFIQQHDF
                     PGVAIDENLQDVNQIQLYHGAPYIFTFGDVDRHN"
     gene            complement(360..443)
                     /locus_tag="ACHOSC_RS19830"
                     /old_locus_tag="pgaptmp_003966"
                     /pseudo
     CDS             complement(360..443)
                     /locus_tag="ACHOSC_RS19830"
                     /old_locus_tag="pgaptmp_003966"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001374575.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin"
     gene            528..614
                     /locus_tag="ACHOSC_RS19835"
                     /old_locus_tag="pgaptmp_003967"
                     /pseudo
     CDS             528..614
                     /locus_tag="ACHOSC_RS19835"
                     /old_locus_tag="pgaptmp_003967"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001343825.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="capsid protein"
     gene            complement(611..859)
                     /locus_tag="ACHOSC_RS19840"
                     /old_locus_tag="pgaptmp_003968"
     CDS             complement(611..859)
                     /locus_tag="ACHOSC_RS19840"
                     /old_locus_tag="pgaptmp_003968"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinI-like family protein"
                     /protein_id="HGU4549802.1"
                     /translation="MRVEICIAKEKISKMPNGAVDALKEELTRRISKRYDDVEVIVKA
                     TSNDGLSVTRTADKDSAKTFVQETLKDTWESADEWFVH"
     gene            complement(1085..1180)
                     /locus_tag="ACHOSC_RS19845"
                     /old_locus_tag="pgaptmp_003969"
                     /pseudo
     CDS             complement(1085..1180)
                     /locus_tag="ACHOSC_RS19845"
                     /old_locus_tag="pgaptmp_003969"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310605.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(1177..1743)
                     /gene="yecD"
                     /locus_tag="ACHOSC_RS19850"
                     /old_locus_tag="pgaptmp_003970"
     CDS             complement(1177..1743)
                     /gene="yecD"
                     /locus_tag="ACHOSC_RS19850"
                     /old_locus_tag="pgaptmp_003970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416381.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrolase"
                     /protein_id="HGU4549803.1"
                     /translation="MLELNAKTTALVVIDLQEGILPFAGGPHTADEVVNRAGKLAAKF
                     RASGQPVFLVRVGWSADYAEALKQPVDAPSPAKVLPENWWQHPAALGATDSDIEIIKR
                     QWGAFYGTDLELQLRRRGIDTIVLCGISTNIGVESTARNAWELGFNLVIAEDACSAAS
                     AEQHNNSINHIYPRIARVRSVEEILHAL"
     gene            2053..3825
                     /gene="aspS"
                     /locus_tag="ACHOSC_RS19855"
                     /old_locus_tag="pgaptmp_003971"
     CDS             2053..3825
                     /gene="aspS"
                     /locus_tag="ACHOSC_RS19855"
                     /old_locus_tag="pgaptmp_003971"
                     /EC_number="6.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004815 - aspartate-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006422 - aspartyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate--tRNA ligase"
                     /protein_id="HGU4549804.1"
                     /translation="MRTEYCGQLRLSHVGQQVTLCGWVNRRRDLGSLIFIDMRDREGI
                     VQVFFDPDRADALKLASELRNEFCIQVTGTVRARDEKNINRDMATGEIEVLASSLTII
                     NRADVLPLDSNHVNTEEARLKYRYLDLRRPEMAQRLKTRAKITSLVRRFMDDHGFLDI
                     ETPMLTKATPEGARDYLVPSRVHKGKFYALPQSPQLFKQLLMMSGFDRYYQIVKCFRD
                     EDLRADRQPEFTQIDVETSFMTAPQVREVMEALVRHLWLEVKGVDLGDFPVMTFAEAE
                     RRYGSDKPDLRNPMELTDVADLLKSVEFAVFAGPANDPKGRVAALRVPGGASLTRKQI
                     DEYGNFVKIYGAKGLAYIKVNERAKGLEGINSPVAKFLNAEIIEAILDRTAAQDGDMI
                     FFGADNKKIVADAMGALRLKVGKDLGLTDESKWAPLWVIDFPMFEDDGEGGLTAMHHP
                     FTSPKDMTAAELKAAPENAVANAYDMVINGYEVGGGSVRIHNGDMQQTVFGILGINEE
                     EQREKFGFLLDALKYGTPPHAGLAFGLDRLTMLLTGTDNIRDVIAFPKTTAAACLMTE
                     APSFANPTALAELSIQVVKKAENN"
     gene            3943..4395
                     /gene="nudB"
                     /locus_tag="ACHOSC_RS19860"
                     /old_locus_tag="pgaptmp_003972"
     CDS             3943..4395
                     /gene="nudB"
                     /locus_tag="ACHOSC_RS19860"
                     /old_locus_tag="pgaptmp_003972"
                     /EC_number="3.6.1.67"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008828 - dATP diphosphatase activity
                     [Evidence IEA];
                     GO_function: GO:0019177 - dihydroneopterin triphosphate
                     pyrophosphohydrolase activity [Evidence IEA];
                     GO_process: GO:0046656 - folic acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroneopterin triphosphate diphosphatase"
                     /protein_id="HGU4549805.1"
                     /translation="MKDKVYKRPVSILVVIYAQDTKRVLMLQRRDDPDFWQSVTGSVE
                     EGETAPQAAMREVKEEVTIDVVAEQLTLIDCQRTVEFEIFSHLRHRYAPGVTRNTESW
                     FCLALPHERQIVFTEHLAYKWLDAPAAAALTKSWSNRQAIEQFVINAA"
     gene            4424..5164
                     /gene="yebC"
                     /locus_tag="ACHOSC_RS19865"
                     /old_locus_tag="pgaptmp_003973"
     CDS             4424..5164
                     /gene="yebC"
                     /locus_tag="ACHOSC_RS19865"
                     /old_locus_tag="pgaptmp_003973"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000907250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HGU4549806.1"
                     /translation="MAGHSKWANTRHRKAAQDAKRGKIFTKIIRELVTAAKLGGGDPD
                     ANPRLRAAIDKALSNNMTRDTLNRAIARGVGGDDDANMETIIYEGYGPGGTAIMIECL
                     SDNRNRTVAEVRHAFSKCGGNLGTDGSVAYLFSKKGVISFEKGDEDTIMEAALEAGAE
                     DVVTYDDGAIDVYTAWEEMGKVRDALEAAGLKADSAEVSMIPSTKADMDAETAPKLMR
                     LIDMLEDCDDVQEVYHNGEISDEVAATL"
     gene            5199..5720
                     /gene="ruvC"
                     /locus_tag="ACHOSC_RS19870"
                     /old_locus_tag="pgaptmp_003974"
     CDS             5199..5720
                     /gene="ruvC"
                     /locus_tag="ACHOSC_RS19870"
                     /old_locus_tag="pgaptmp_003974"
                     /EC_number="3.1.21.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005068993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008821 - crossover junction DNA
                     endonuclease activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="crossover junction endodeoxyribonuclease RuvC"
                     /protein_id="HGU4549807.1"
                     /translation="MAIILGIDPGSRVTGYGVIRQVGRQLSYLGSGCIRTKVDDLPSR
                     LKLIYAGVTEIITQFQPDYFAIEQVFMAKNADSALKLGQARGVAIVAAVNQELPVFEY
                     AARQVKQTVVGIGSAEKSQVQHMVRTLLKLPANPQADAADALAIAITHCHVSQNAMQM
                     SESRLNLARGRLR"
     gene            complement(5722..6324)
                     /gene="yebB"
                     /locus_tag="ACHOSC_RS19875"
                     /old_locus_tag="pgaptmp_003975"
     CDS             complement(5722..6324)
                     /gene="yebB"
                     /locus_tag="ACHOSC_RS19875"
                     /old_locus_tag="pgaptmp_003975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416376.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebB family permuted papain-like enzyme"
                     /protein_id="HGU4549808.1"
                     /translation="MNINYPAEYEIGDIVFTCIGAALFGQISAASNCWSNHVGIIIGH
                     NGEDFLVAESRVPLSTITTLSRFIKRSANQRYAIKRLDAGLTEQQKQRIVEQVPSRLR
                     KLYHTGFKYESSRQFCSKFVFDIYKEALCIPVGEIETFGQLLNSNPNAKLTFWKFWFL
                     GSIPWERKTVTPASLWHHPGLVLIHAEGVETPQPELTEAV"
BASE COUNT         1624 a         1513 c         1690 g         1550 t
ORIGIN      
        1 ggcatgaaaa gaatgagagg gctttcttcg aaagactggg tacgatcagt gaccgatatg
       61 aggcgagtcc tgatttcaca gagtattccg ctgtgtctaa cataggatac ggatttatcc
      121 agcaacatga ttttccaggg gtggctatcg acgagaattt acaggatgta aatcagatcc
      181 aactgtatca tggtgctcct tatatcttta catttgggga tgtggacaga cacaattagc
      241 gctgattcgt ctttacagtt acatagggtt actactcata gatattaacg gagttgatga
      301 tgggcaatcg tgcaacattg tattgtatgt aaaggcagag ccccgaattt tactgttgta
      361 agtgggcctg gcgggaatgg tatcgccagc atcattggct tgggtccagt aaaaagagac
      421 gcagaaagat gcacaatagg cactacgcgt catgcatgga ttaggtcgct catcatttac
      481 acagtactgc taaaaaatta ttaaggaaag cgcggcactt cgtatgtaag aacgtgtcgc
      541 ggttggctgg tgaactttcg atagtgcgag tattgaatga tttccagccg ttaccgattt
      601 tacgtgttta ttagtgaaca aaccactcgt cagcagactc ccaggtatct ttcagagtct
      661 cctgaacaaa tgtttttgca gaatctttat ctgcggtgcg tgtaacagaa agcccatcgt
      721 tgctggtggc ttttacgatc acctctacat cgtcataacg tttactgatg cgtcgggtta
      781 attcttcctt taacgcatcc acagcaccgt ttggcatttt gctgattttt tctttagcaa
      841 tacagatctc aacacgcata acaccctcct gtaactgtgg ttatatacag gtgttatttt
      901 tatctgtatg gataaccagt gtcaattcct gatattgttt atggggcatc aatggggcat
      961 gtatgggaca ctttttatcg gcgaaattcg tcgaagttcg tcgacatggt aaacgaatca
     1021 tctatccaac ccttgaaaaa cggcgctcct ggacgatctt cgtcgatttt taaaaatgtt
     1081 gcgtcatgca gttaaagtgg cgggcatact cttcaaggct ggtgatcccc aaccgcaccc
     1141 atttaggatg cgaccattgc ggtagaccga tgtaaatcat aacgcgtgaa ggatctcttc
     1201 cacgctacgc acacgggcga tgcgtgggta gatatggttg atgctgttat tgtgctgctc
     1261 ggcgctagcg gcactacagg cgtcttcggc aatcaccaga ttaaagccaa gttcccaggc
     1321 attgcgggcg gtggattcaa caccgatatt ggtcgagatc ccacataaca ctattgtatc
     1381 gatacctcgg cggcgcagtt gcaactccag atccgtaccg taaaacgcac cccactgacg
     1441 tttgatgatt tcgatatcac tgtcagttgc acctaatgca gctggatgtt gccaccagtt
     1501 ttcgggcaac actttagcgg gggagggggc atcaaccggc tgttttaatg cttcggcgta
     1561 atcggcagac cagccaacgc gcaccagaaa cacgggctga ccgctggcgc gaaatttcgc
     1621 cgccagcttc ccggcgcgat taactacctc atcggcagta tgtggaccac cggcaaaagg
     1681 taagatgcct tcttgtaaat cgatcaccac cagcgcagtg gttttagcat taagttcaag
     1741 cataatgact cctgaaaaaa caaaatcgtg cctcacacct taatgcgatt agccgatggc
     1801 ggaacgacaa attttgttaa tttttatgag ttcgggcaac acctttatag caaactggcg
     1861 tagagtctga tttcaggccc gatataaagt ggtaacgagg cgttccagcg cgggccaggc
     1921 tcttacggtg cggcagaatt tccagtataa tagccgcctt ttttcattca gttgtgacat
     1981 acagctaacg ctgcgacttg gtcacctgcg cagcaggggg catccgccgg cagctaagtt
     2041 aagggatatc tcatgcgtac agaatattgt ggacagctcc gtttgtccca cgtggggcag
     2101 caggtgactc tgtgtggttg ggtcaaccgt cgtcgtgatc ttggtagcct gatcttcatc
     2161 gatatgcgcg accgcgaagg tatcgtgcag gtatttttcg atccggatcg tgcggacgcg
     2221 ttaaagctgg cctctgaact gcgtaatgag ttctgcattc aggtcacggg caccgtacgt
     2281 gcgcgtgacg aaaaaaatat taaccgcgat atggcgaccg gcgaaatcga agtgctggcg
     2341 tcctcgctga ctatcatcaa ccgcgcagat gttctgccgc ttgactctaa ccacgtcaac
     2401 accgaagaag cgcgtctgaa ataccgctac ctcgacctgc gtcgtccgga aatggctcag
     2461 cgcctgaaaa cccgcgctaa aatcaccagc ctggtgcgcc gttttatgga tgaccacggc
     2521 ttcctcgaca tcgaaactcc gatgctgacc aaagccacgc cggaaggcgc gcgtgactac
     2581 ctggtgcctt ctcgtgtgca caaaggtaaa ttctacgcac tgccgcaatc cccacagttg
     2641 ttcaaacagc tgctgatgat gtccggtttt gaccgttact atcagatcgt taaatgcttc
     2701 cgtgacgaag acctgcgcgc tgatcgtcag cctgaattta ctcagatcga tgtggaaact
     2761 tctttcatga ccgcgccgca agtgcgtgaa gtgatggaag cgctggtgcg tcatttgtgg
     2821 ctggaagtga agggcgtgga tctgggcgat ttcccggtaa tgacctttgc ggaagcagaa
     2881 cgccgttatg gttctgataa accggatctg cgtaacccga tggaactgac cgacgttgcc
     2941 gatctgctga aatctgttga gtttgcagta tttgcggggc cggcgaacga tccgaaaggc
     3001 cgtgttgccg ctctacgtgt tccgggtggc gcatcgctga cccgtaagca gatcgacgaa
     3061 tacggtaact tcgttaaaat ctacggcgcg aaaggtctgg cttacatcaa agttaacgaa
     3121 cgcgcgaaag gtctggaagg tatcaacagc ccggtagcga agttccttaa tgcagaaatc
     3181 atcgaagcca tcctggatcg tactgccgcg caagatggcg atatgatttt cttcggtgcc
     3241 gacaacaaga aaattgttgc cgacgcgatg ggcgcactgc gcctgaaagt gggtaaagac
     3301 cttggtctga ccgacgaaag caaatgggca ccgctgtggg ttatcgactt cccgatgttt
     3361 gaagacgacg gtgaaggcgg cctgacggca atgcaccatc cgttcacctc accgaaagac
     3421 atgactgctg cagaactgaa agctgcaccg gaaaatgcgg tggcgaacgc ttacgatatg
     3481 gtcatcaatg gttacgaagt gggcggtggt tcagtacgta tccataatgg tgatatgcag
     3541 cagacggtgt ttggtattct gggtatcaac gaagaggaac agcgcgagaa attcggcttc
     3601 ctgctcgacg ccctgaaata cggtactccg ccgcacgcag gtctggcatt cggtcttgac
     3661 cgtctgacca tgctgctgac tggcaccgac aatatccgtg acgttatcgc cttcccgaaa
     3721 accacggcgg cagcgtgtct gatgactgaa gcaccgagct ttgctaaccc gactgcactg
     3781 gctgagctga gcattcaggt tgtgaagaag gctgagaata actgatatga ctcaaataca
     3841 cgaaatcatt cgcgttgcat caaggcggca actgagtgaa ctcccatgag catagataac
     3901 tatgtgaatg ggatgagcga aggcagtcaa cgaagaggca gcgtgaagga taaagtgtat
     3961 aagcgtcccg tttcgatctt agtggtcatc tatgcacaag atacgaaacg ggtgctgatg
     4021 ttgcagcggc gtgacgatcc cgatttctgg cagtcggtaa ccggcagcgt ggaagagggt
     4081 gaaaccgcgc cgcaagctgc catgcgcgaa gtaaaggaag aggtcaccat tgatgttgtc
     4141 gctgaacaac tgaccttaat tgactgtcag cgcacggtag agtttgaaat tttttcacat
     4201 ttacgtcatc gctatgcgcc gggcgtgacg cgtaatacgg aatcatggtt ctgtcttgcg
     4261 cttccgcacg agcggcagat cgttttcact gaacatctgg cttacaagtg gcttgatgcg
     4321 cctgctgcgg cggcgctcac taagtcctgg agcaaccggc aggcgattga acagtttgtc
     4381 attaacgctg cctgaacagg cagggctatc tggagatatt tttatggcag gtcatagtaa
     4441 atgggccaac accagacatc gtaaagctgc gcaggatgca aagcgcggta aaatcttcac
     4501 taaaatcatt cgtgagctgg taaccgcggc taagctgggc ggtggcgatc cggacgctaa
     4561 cccgcgtctg cgtgcagcaa ttgataaagc actgtctaac aacatgaccc gcgacacact
     4621 gaaccgcgca attgcacgtg gtgtgggcgg tgatgatgat gcaaacatgg aaaccatcat
     4681 ctacgaaggt tacggtcctg gcggcacggc aatcatgatt gaatgtctgt ctgacaaccg
     4741 caaccgtacc gttgctgaag tgcgtcatgc attcagcaaa tgtggcggta acctcggtac
     4801 tgatggttcc gtagcctatc tgttcagcaa aaaaggcgtg atctccttcg agaaaggcga
     4861 tgaagacacc atcatggaag cagctttgga agcaggtgct gaagacgttg tgacctatga
     4921 tgacggcgcg attgatgtct acaccgcatg ggaagaaatg ggtaaagtgc gcgacgctct
     4981 ggaagcggca ggtctgaaag cagacagcgc ggaagtttcc atgatcccgt ccaccaaagc
     5041 tgatatggat gcagaaaccg caccgaaact gatgcgtctg atcgatatgc tggaagattg
     5101 cgacgacgtg caggaagttt accataacgg tgaaatctct gatgaggttg cagcgactct
     5161 ctgatgaggc ctgctaaaca gcaaaacgga gacgcgtgat ggctattatt ctcggcattg
     5221 atccgggttc gcgcgtgacc ggctatggcg tcatccgcca ggtaggcagg caactgtcct
     5281 acctgggtag tggatgcata cgcaccaaag tggatgattt accgtctcgt ctgaagctca
     5341 tctatgcggg cgtgacggaa atcatcaccc agttccagcc tgattatttc gccattgaac
     5401 aagtctttat ggcgaagaac gctgactcag ccctgaaact gggccaggcg cgcggcgtgg
     5461 cgattgtggc ggcggtgaat caggaattgc cggtatttga atacgcggca cgtcaggtaa
     5521 agcaaacggt ggtaggtatt ggtagcgccg agaaaagcca ggtgcagcat atggtccgca
     5581 ccttgctgaa actgcccgct aatccacaag cggatgccgc tgatgcgtta gctatcgcga
     5641 ttacccattg tcacgtcagc cagaatgcaa tgcagatgag cgaatcgcgg ctgaacctgg
     5701 cgagagggcg actgcgttaa gttataccgc ctcggtcagt tcaggctgag gcgtttcaac
     5761 tccctccgcg tgaatcaaca ccaaacccgg atgatgccac aaactggctg gcgtgacggt
     5821 tttacgctcc cacggaatag aacctaagaa ccagaatttc cagaaagtga gttttgcatt
     5881 cggattgcta tttaacagtt gtccaaacgt ctctatttca cccaccggaa tacatagcgc
     5941 ctctttataa atatcaaaga caaattttga acagaactgg cgggaagatt cgtatttaaa
     6001 acccgtgtgg taaagtttgc gtagccggga gggaacctgt tcaacaattc gttgtttctg
     6061 ttgttctgtc agcccggcgt ctaatcgctt tatagcatag cgttgattag cagagcgttt
     6121 aataaaacgg gatagcgtag tgatggttga gaggggaaca cggctttctg caaccagaaa
     6181 gtcttcaccg ttatgaccga taatgatccc gacgtgatta ctccagcagt ttgatgcagc
     6241 tgatatttga ccaaataagg cagcacctat acatgtaaag acgatatcac caatttcata
     6301 ttcagcaggg taattaatat tcacgttgtc attccattga aatagatatg aatatacgta
     6361 atagcataaa aaattta
//
LOCUS       DAZKHF020000246         6368 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2201S31241
            SAMN43267800-rid25106413.denovo.248, whole genome shotgun sequence.
ACCESSION   DAZKHF020000246 DAZKHF020000000
VERSION     DAZKHF020000246.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267800
            Sequence Read Archive: SRR30307263
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6368)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6368)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 6368)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350581.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350581.2
            Genome Coverage       :: 151x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:21:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,652
            CDSs (total)                      :: 5,557
            Genes (coding)                    :: 5,320
            CDSs (with protein)               :: 5,320
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 237
            CDSs (without protein)            :: 237
            Pseudo Genes (ambiguous residues) :: 0 of 237
            Pseudo Genes (frameshifted)       :: 102 of 237
            Pseudo Genes (incomplete)         :: 130 of 237
            Pseudo Genes (internal stop)      :: 52 of 237
            Pseudo Genes (multiple problems)  :: 41 of 237
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6368
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267800-rid25106413.denovo.248"
                     /serotype="O 26:H11"
                     /isolate="2201S31241"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-01"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            40..864
                     /gene="rcnA"
                     /locus_tag="ACHOSC_RS19880"
                     /old_locus_tag="pgaptmp_003976"
     CDS             40..864
                     /gene="rcnA"
                     /locus_tag="ACHOSC_RS19880"
                     /old_locus_tag="pgaptmp_003976"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nickel/cobalt efflux protein RcnA"
                     /protein_id="HGU4549809.1"
                     /translation="MTEFTTLLQQGNAWFFIPSAILLGALHGLEPGHSKTMMAAFIIA
                     IKGTIKQAVMLGLAATISHTAVVWLIAFGGMVVSKRFTAQSAEPWLQLISAVIIISTA
                     FWMFWRTWHGERNWLENMHEHDHEHHHHDHEDHHDHGHHHHHEHGEYQDAHARAHAND
                     IKRRFDGREVTNWQILLFGLTGGLIPCPAAITVLLICIQLKALTLGATLVVSFSIGLA
                     LTLVTVGVGAAISVQQVAKRWSGFNTLAKRAPYFSSLLIGLVGVYMGVHGFMGIMR"
     gene            1083..1421
                     /gene="rcnB"
                     /locus_tag="ACHOSC_RS19885"
                     /old_locus_tag="pgaptmp_003977"
     CDS             1083..1421
                     /gene="rcnB"
                     /locus_tag="ACHOSC_RS19885"
                     /old_locus_tag="pgaptmp_003977"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416610.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ni(II)/Co(II) efflux transporter accessory
                     subunit RcnB"
                     /protein_id="HGU4549810.1"
                     /translation="MTIKNKMLLGALLLVTSAAWAAPATAGSTNTSGISKYELSSFIA
                     DFKHFKPGDTVPEMYRTDEYNIKQWQLRNLPAPDAGTHWTYMGGAYVLISDTDGKIIK
                     AYDGEIFYHR"
     gene            complement(1503..2537)
                     /gene="yehA"
                     /locus_tag="ACHOSC_RS19890"
                     /old_locus_tag="pgaptmp_003978"
     CDS             complement(1503..2537)
                     /gene="yehA"
                     /locus_tag="ACHOSC_RS19890"
                     /old_locus_tag="pgaptmp_003978"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative fimbrial-like adhesin protein"
                     /protein_id="HGU4549811.1"
                     /translation="MNIRIMLFIVMMMVMPVSYAACYSELSVQHNLVVQGDFALTQTQ
                     MATYEHNFNDSSCVSTNTITPMSPSDIIVGLYNDTIKLNLHFEWTNKNNITLSNNQTS
                     FTSGYSVTVTPAASNAKVNVSAGGGGSVMINGVATLSSASSSTRGSAAVQFLLCLLGG
                     KSWDACVNSYRNALAQNAGVYSFNLTLSYNPITTTCKPDDLLITLDSIPVSQLPATGN
                     KATINSKQGDIILRCKNLLGQQNQTSRKMQVYLSSSDLLTNSNTILKGAEDNGVGFIL
                     ESNGSSVTLLNITNSSKRYTNLKEIAAKSKLTDTTVSIPITASYYVYDTNKVKSGALE
                     ATALINVKYD"
     gene            complement(2551..5031)
                     /gene="yehB"
                     /locus_tag="ACHOSC_RS19895"
                     /old_locus_tag="pgaptmp_003979"
     CDS             complement(2551..5031)
                     /gene="yehB"
                     /locus_tag="ACHOSC_RS19895"
                     /old_locus_tag="pgaptmp_003979"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial biogenesis outer membrane usher
                     protein"
                     /protein_id="HGU4549812.1"
                     /translation="MLRMTPLASAIVALLLGIEAYAAEETFDTHFMIGGMKDQQVANI
                     RLDDNQPLPGQYDIDIYVNKQWRGKYEIIVKDNPQETCLSIEVIKRLGINSDNFASGK
                     QCLTFEQLVQGGSYTWDIGVFRLDFSVPQAWVEELESGYVPPENWERGINAFYTSYYV
                     SQYYSDYKASGNSKSTYVRFNSGLNLLGWQLHSDASFSKTNNNPGVWKSNTLYLERGF
                     AQLLGTLRVGDMYTSSDIFDSVRFSGVRLFRDMQMLPNSKQNFTPRVQGIAQSNALVT
                     IEQNGFVVYQKEVPPGPFAITDLQLAGGGADLDVSVKEADGSVTTYLVPYAAVPNMLQ
                     PGVSKYDFAAGRSHIEGASKQSDFVQAGYQYGFNNLLTLYGGSMVANNYYAFTLGTGW
                     NTRIGAISVDATKSHSKQDNGDVFDGQSYQIAYNKFVSQTSTRFGLAAWRYSSRDYRT
                     FNDHVWANNKDNYRRDENDVYDIADYYQNDFGRKNSFSANMSQSLPEGWGSVSLSTLW
                     RDYWGRSGSSKDYQLSYSNNWRRISYILAASQAYDENHHEEKRFNIFISIPFDWGDDV
                     TTPRRQIYMSNSTTFDDQGFASNNTGLSGTVGSRDQFNYGVNLSYQNQGNETTAGANL
                     TWNAPVATVNGSYSQSSTYRQAGASVSGGIVAWSGGVNLANRLSETFAVMNAPGIKDA
                     YVNGQKYRTTNRNGVVVYDGMTPYRENHLMLDVSQSDSEAELRGNRKIAASYRGAVVL
                     VNFDTDQRKPWFIKALRADGQPLMFGYEVNDIHGHNIGVVGQGSQLFIRTNEVPPSVN
                     VAIDKQQGLSCTITFGKEIDESRNYICQ"
     gene            complement(5047..5721)
                     /gene="yehC"
                     /locus_tag="ACHOSC_RS19900"
                     /old_locus_tag="pgaptmp_003980"
     CDS             complement(5047..5721)
                     /gene="yehC"
                     /locus_tag="ACHOSC_RS19900"
                     /old_locus_tag="pgaptmp_003980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbria/pilus periplasmic chaperone"
                     /protein_id="HGU4549813.1"
                     /translation="MKGLLSLLIFSMVLPAHAGIVIYGTRIIYPAENKEVMVQLMNQG
                     NRSSLLQAWIDDGDTSLPPEKIQVPFMLTPPVAKIGANSGQQVKIKIMPNKLPTNKES
                     IFYLNVLDIPPNSPEQEGKNALKFAMQNRIKLFYRPAGIAPVNKATFKKLLVNRSGNG
                     LVIKNDSANWVTISDVKANNVKVNYETIMIAPLESQSVNVKSNNANNWYLTIIDDHGN
                     YISDKI"
     gene            complement(5802..6344)
                     /gene="yehD"
                     /locus_tag="ACHOSC_RS19905"
                     /old_locus_tag="pgaptmp_003981"
     CDS             complement(5802..6344)
                     /gene="yehD"
                     /locus_tag="ACHOSC_RS19905"
                     /old_locus_tag="pgaptmp_003981"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HGU4549814.1"
                     /translation="MKRSIIAAAVFSSFFMSAGVFAADVDTGTLTIKGNIAESPCKFE
                     AGGDSVSINMPTVPTTVFEGKAKYSTYDDAVGVTSSMLKISCPKEVAGVKLSLITNDK
                     ITGNDKEIASSNDTVGYYLYLGDNSDVLDVSAPFNIESYKTAEGQYAIPFKAKYLKLT
                     DNSVQSGDVLSSLVMRVAQD"
BASE COUNT         1734 a         1521 c         1259 g         1854 t
ORIGIN      
        1 tagaatcaga ttgccgaatt aatactaaga attattatca tgaccgaatt tacaactctt
       61 cttcagcaag gaaacgcctg gttcttcatc cccagcgcca tcttacttgg tgcgcttcat
      121 ggcctggaac cagggcactc aaaaacgatg atggcggcgt ttatcatcgc catcaaaggc
      181 accattaaac aggcggtgat gctcggactg gcagcaacta tttcgcatac cgcagtggtc
      241 tggttgattg cctttggcgg gatggtggtc agcaagcgct ttactgctca atcagcagaa
      301 ccgtggctcc agttgatttc cgcagtgatc attattagca ccgcgttctg gatgttctgg
      361 cgtacctggc acggcgagcg caactggctg gagaatatgc acgagcatga tcatgagcat
      421 catcatcacg atcacgaaga tcaccacgac catggacatc atcaccatca cgaacatggc
      481 gagtatcagg atgctcatgc acgagcccat gctaatgaca ttaaacgacg ctttgatggt
      541 agagaggtca ccaactggca aattttgtta tttggcttaa ccggtggcct tatcccctgc
      601 ccggcagcaa ttaccgtgct gttgatatgc attcagttga aagccctgac actgggcgca
      661 acactggtcg tcagtttcag cattggcctg gcgttaacgc ttgtcaccgt aggcgttggc
      721 gcagcaatca gcgttcagca ggtcgcaaaa cgctggagcg gatttaacac tctcgctaaa
      781 cgcgccccct atttttccag tctgttgatt ggcttagtcg gtgtgtatat gggcgtacat
      841 ggcttcatgg gcataatgcg ataactcaat cctttaggct tgcatgttat cttcagccca
      901 ggatgattct taaatcagct atttccgctg actctctgct cgctaaggat gcttttaggg
      961 catcctttca tttacacttt ttacgaaatc atgggatcac taacaaaata tcgcttgtca
     1021 gttatattgt atggcaggaa agatatgcga ctgatattac agatccccaa agtggagagt
     1081 ttatgaccat taaaaataag atgttgctgg gtgcgctttt gctggttacc agtgccgcct
     1141 gggccgcacc agccaccgcg ggttcgacca atacctcggg aatttctaag tatgagttaa
     1201 gcagtttcat tgctgacttt aagcatttca aaccaggcga taccgtccca gagatgtacc
     1261 gcaccgatga gtacaacatt aagcagtggc agttacgtaa cctgcccgcg cctgatgccg
     1321 ggacgcactg gacctatatg ggtggcgcgt acgtgttgat cagcgacacc gacggtaaaa
     1381 tcattaaagc ctacgacggt gagatttttt atcatcgcta aaaaaagccc cctcatcatg
     1441 agggggaaat gcagacacct tgttattttt tattattagc cacttgctcg tcttgcttgt
     1501 tattagtcgt atttcacgtt gattaatgcg gttgcctcca gtgcgccaga tttaactttg
     1561 tttgtatcgt agacgtagta actggcagtt atcggaattg aaaccgttgt atctgtaagt
     1621 tttgactttg ccgcaatttc ttttaaattt gtgtatcgtt tactgctgtt agtgatattt
     1681 aaaagtgtga ctgacgaacc attactttca agaataaatc ctacgccatt atcttccgca
     1741 cctttcagta ttgtgttgct gttggttaac aagtcagaac ttgataaata cacctgcatt
     1801 ttccgtgatg tttgattttg ttgacctaat aaatttttac aacgcagaat aatatcccct
     1861 tgtttactat ttattgttgc tttgttacct gtggctggta attgtgaaac gggaatactg
     1921 tctaaagtaa ttaataaatc gtccggtttg caggttgtgg ttatcgggtt gtatgacaat
     1981 gtcagattaa aggaatagac acctgcattt tgtgccaatg catttctgta gctatttaca
     2041 catgcatccc atgacttgcc acctaataaa cacagtagaa attgtactgc ggcactcccg
     2101 cgtgtcgatg atgaagcact ggataatgtc gcaactccat taatcatcac tgaaccgccg
     2161 ccccccgcag aaacattcac ttttgcatta ctggccgcag gtgtcaccgt taccgaataa
     2221 ccactggtga aactggtctg attatttgat aacgtgatgt tgtttttatt ggtccattca
     2281 aaatgtaaat ttaatttaat ggtatcgtta taaagtccaa caataatatc cgacgggctc
     2341 ataggggtga tagtatttgt actcacgcat gacgaatcat taaaattatg ctcatatgtc
     2401 gccatttgtg tttgagtaag tgcaaaatcc ccctgaacaa ccaaattgtg ctgaacagat
     2461 aactcactat aacatgccgc atagctcaca ggcataacca tcatcattac tataaataac
     2521 attatacgaa tattcataac cacctgacta ttactggcaa atatagtttc tactttcatc
     2581 aatctcttta ccgaaggtga ttgtgcatga aagtccttgt tgcttatcaa ttgctacatt
     2641 aaccgatggc ggtacttcat tggtgcgaat aaataactgg cttccctgac cgacaacgcc
     2701 aatattatga ccatggatat cattgacttc ataaccaaac attaatggtt gcccatccgc
     2761 tcttaacgct tttataaacc agggcttgcg ctgatcggta tcaaaattaa ccagtacaac
     2821 cgcgccgcga taagaggcgg caattttccg gttgccacgt aattctgctt cgctatcgct
     2881 ttgcgacaca tccagcatca ggtgattttc ccgataaggt gtcattccgt cgtataccac
     2941 cactccatta cggtttgttg tgcgatattt ttgcccattg acataagcat ctttaattcc
     3001 tggcgcattc atcacagcaa acgtttcgga aagacggttc gccagattaa cgccccccga
     3061 ccaggcgaca atgccccctg aaacactggc tccagcctgt cgataagtac tcgactgact
     3121 ataactgcca ttcactgtcg caaccggcgc gttccaggtc aaattcgccc cagctgtcgt
     3181 ttcatttccc tgattctgat aactcaggtt gaccccatag ttaaactgat cccggcttcc
     3241 aacggttcct gataatcccg tattatttga ggcaaacccc tgatcatcaa acgtcgttga
     3301 gttagacata tatatttgcc gacgaggcgt cgtaacgtca tcaccccaat caaagggaat
     3361 cgatataaaa atattaaaac gtttctcttc atgatgattc tcgtcatacg cctggcttgc
     3421 cgcgaggata tagcttatcc gccgccagtt attggaataa ctcaactgat aatccttact
     3481 gctgccgcta cgcccccagt aatctcgcca taacgtactt aaggacacag acccccagcc
     3541 ttctggcaat gactggctca tattggcaga gaagctattt ttacggccaa aatcgttctg
     3601 gtaataatcg gcaatgtcat agacatcgtt ttcatcacgg cgataattat ctttattgtt
     3661 tgcccaaacg tgatcattaa atgtccggta atcacgcgat gaataacgcc aggccgccag
     3721 accaaaacgc gtcgacgttt ggctcacaaa tttgttgtag gcaatttgat aactttgccc
     3781 gtcaaacaca tcgccgttgt cttgtttact atgcgacttc gtggcatcga cggaaatggc
     3841 accaatgcgt gtattccagc cagtccccaa agtaaacgcg taataattat tcgcgaccat
     3901 cgagccacca tacagcgtca ataaattatt aaaaccatac tgataacccg cctggacaaa
     3961 atcactttgt ttgctcgccc cttcaatatg gctacgaccc gccgcaaaat catatttcga
     4021 tacgccgggt tgcagcatat ttggcactgc tgcataaggc accagatagg tggttaccga
     4081 gccgtccgcc tctttcacgc tgacatcaag atctgctcca ccaccggcca actgcaaatc
     4141 tgtaatcgcg aacgggccag gaggaacctc tttctgataa accacaaaac cgttctgttc
     4201 aatagttacc agcgcattac tctgagcaat cccctgcacc cgtggcgtaa aattttgttt
     4261 cgagttaggc aacatctgca tatcacgaaa caaccgcaca ccgctgaagc gaacagaatc
     4321 aaaaatatcg cttgatgtgt acatatcacc cacgcgaagc gtgccgagaa gttgggcaaa
     4381 tccacgttcc agatacaggg tattgctttt ccacacccct ggattgttat ttgttttact
     4441 gaaactggca tcagaatgca gttgccaccc cagtaaattt aacccgctgt taaaacgtac
     4501 atatgtactc ttactattac ccgacgcttt atagtcactg taatactgac tcacataata
     4561 agaggtataa aacgcattaa taccccgctc ccagttttcc ggtggaacat agccactttc
     4621 cagttcttcc acccaggcct gcgggacact gaaatcgaga cgaaaaaccc cgatatccca
     4681 ggtatagctc ccaccctgaa caagttgctc aaatgttaaa cattgcttac cgctggcgaa
     4741 gttatcgcta ttaatgccta accgcttgat aacttctatt gataaacatg tttcttgcgg
     4801 gttgtcttta acaataatct catatttccc gcgccattgc ttattgacat aaatatcgat
     4861 gtcatactgc cccggtaagg gttgattatc atcaagacga atatttgcaa cctgctggtc
     4921 tttcattcca cctatcataa aatgggtatc aaaggtttct tcagctgcat aagcttcaat
     4981 gccgagcaat aacgctacga ttgctgatgc aagtggagtc attctcaaca taggcagctc
     5041 ctgcaattaa attttgtcac taatatagtt gccgtggtca tcgataatgg tcagatacca
     5101 gttatttgca ttattacttt tgacattaac actctgactt tctaaggggg caatcataat
     5161 agtttcataa ttgactttaa cattattagc tttgacatcc gaaatcgtca cccaattagc
     5221 tgagtcattt tttatcacca atccattgcc actgcgattt accagtaact ttttaaaggt
     5281 cgctttattt accggagcaa tacccgctgg ccggtaaaac aacttaattc tgttttgcat
     5341 cgcaaacttc agtgcatttt tgccttcttg ctctggacta tttggtggaa tatccagaac
     5401 attcagataa aaaatgcttt ctttattagt gggcagttta ttcggcataa ttttgatttt
     5461 tacttgctgc ccggaatttg cccctatttt tgccactggt ggcgttaaca tgaaaggaac
     5521 ctgaattttt tctggtggta acgacgtatc gccatcatca atccacgcct gcagcagcga
     5581 agaacggttt ccctggttca tcaactgcac cataacttct ttattttctg ccgggtaaat
     5641 aatgcgcgtc ccgtagataa caattccggc atgtgcagga aggaccatag aaaaaatgag
     5701 taaagataat aatcctttca tcttaatgcc tcttaaatta aaaagccgcc atggggatag
     5761 cggccatgtt tattacctgg agaaacttac aattttattt attaatcctg cgccacacgc
     5821 ataaccagag aagataacac atcacctgat tgcactgagt tatctgtcag tttcaggtat
     5881 tttgctttaa acggaatagc atattgacct tccgctgttt tataactctc aatgttaaaa
     5941 ggtgcagaaa catccaggac atcgctgtta tcacctaaat agagatagta acccacggta
     6001 tcgttgctac tggctatctc cttatcgtta ccggttattt tatcgttggt aatcaacgag
     6061 agttttacac cagcaacttc tttcgggcag ctaattttta acatgctgct ggttacaccg
     6121 actgcatcat cataggtaga atatttagct ttaccttcaa agacagtggt tggtacagtc
     6181 ggcatattaa tacttactga atcaccaccc gcttcgaatt tacacggaga ttctgcaata
     6241 ttccccttaa tagttaatgt tccggtatca acgtctgcag caaatactcc agcgctcata
     6301 aaaaaagaag aaaagacagc ggcagcaata attgaacgtt tcataatatg ttccttaata
     6361 aaatttat
//
LOCUS       DAZKHF020000247         6282 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2201S31241
            SAMN43267800-rid25106413.denovo.249, whole genome shotgun sequence.
ACCESSION   DAZKHF020000247 DAZKHF020000000
VERSION     DAZKHF020000247.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267800
            Sequence Read Archive: SRR30307263
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6282)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6282)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 6282)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350581.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350581.2
            Genome Coverage       :: 151x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:21:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,652
            CDSs (total)                      :: 5,557
            Genes (coding)                    :: 5,320
            CDSs (with protein)               :: 5,320
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 237
            CDSs (without protein)            :: 237
            Pseudo Genes (ambiguous residues) :: 0 of 237
            Pseudo Genes (frameshifted)       :: 102 of 237
            Pseudo Genes (incomplete)         :: 130 of 237
            Pseudo Genes (internal stop)      :: 52 of 237
            Pseudo Genes (multiple problems)  :: 41 of 237
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6282
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267800-rid25106413.denovo.249"
                     /serotype="O 26:H11"
                     /isolate="2201S31241"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-01"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            complement(79..855)
                     /gene="yaaA"
                     /locus_tag="ACHOSC_RS19910"
                     /old_locus_tag="pgaptmp_003982"
     CDS             complement(79..855)
                     /gene="yaaA"
                     /locus_tag="ACHOSC_RS19910"
                     /old_locus_tag="pgaptmp_003982"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxide stress protein YaaA"
                     /protein_id="HGU4549815.1"
                     /translation="MLILISPAKTLDYQSPLTTTRYTLPELLDNSQQLIHEARKLTPP
                     QISTLMRISDKLAGINAARFHDWQPDFTPENARQAILAFKGDVYTGLQAETFSEDDFD
                     FAQQHLRMLSGLYGVLRPLDLMQPYRLEMGIRLENARGKDLYQFWGDIITNKLNEALA
                     AQGDNVVINLASDEYFKSVKPKKLNAEIIKPVFLDEKNGKFKIISFYAKKARGLMSRF
                     IIENRLTKPEQLTGFNSEGYFFDEASSSNGELVFKRYEQR"
     gene            complement(925..2355)
                     /locus_tag="ACHOSC_RS19915"
                     /old_locus_tag="pgaptmp_003983"
     CDS             complement(925..2355)
                     /locus_tag="ACHOSC_RS19915"
                     /old_locus_tag="pgaptmp_003983"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005283 - amino acid:sodium symporter
                     activity [Evidence IEA];
                     GO_function: GO:0015293 - symporter activity [Evidence
                     IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine/glycine:cation symporter family protein"
                     /protein_id="HGU4549816.1"
                     /translation="MPDFFSFINSVLWGSVMIYLLFGAGCWFTFRTGFVQFRYIRQFG
                     KSLKNSIHPQPGGLTSFQSLCTSLAARVGSGNLAGVALAITAGGPGAVFWMWVAAFIG
                     MATSFAECSLAQLYKERDANGQFRGGPAWYMARGLGMRWMGVLFAVFLLIAYGIIFSG
                     IQANAVARALSFSFDFPPLVTGIILAVFALLAITRGLHGVARLMQGFVPLMAIIWVLT
                     SLVICVMNIGQLPHVIWSIFESAFGWQEAAGGAAGYTLSQAITNGFQRSMFSNEAGMG
                     STPNAAAAAASWPPHPAAQGIVQMIGIFIDTLVICTASAMLILLAGNGTTYMPLEGIQ
                     LIQKAMRVLMGSWGAEFVTLVVILFAFSSIVANYIYAENNLFFLRLNNPKAIWCLRIC
                     TFATVIGGTLLSLPLMWQLADIIMACMAITNLTAILLLSPVVHTIASDYLRQRKLGVR
                     PVFDPLRYPDIGRQLSPDAWDDVSQE"
     gene            2634..3587
                     /gene="tal"
                     /locus_tag="ACHOSC_RS19920"
                     /old_locus_tag="pgaptmp_003984"
     CDS             2634..3587
                     /gene="tal"
                     /locus_tag="ACHOSC_RS19920"
                     /old_locus_tag="pgaptmp_003984"
                     /EC_number="2.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004801 - transaldolase activity [Evidence
                     IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transaldolase"
                     /protein_id="HGU4549817.1"
                     /translation="MTDKLTSLRQYTTVVADTGDIAAMKLYQPQDATTNPSLILNAAQ
                     IPEYRKLIDDAVAWAKQQSNDRAQQIVDATDKLAVNIGLEILKLVPGRISTEVDARLS
                     YDTEASIAKAKRLIKLYNDAGISNDRILIKLASTWQGIRAAEQLEKEGINCNLTLLFS
                     FAQARACAEAGVFLISPFVGRILDWYKANTDKKEYAPAEDPGVVSVSEIYQYYKEHGY
                     ETVVMGASFRNIGEILELAGCDRLTIAPTLLKELAESEGAIERKLSYTGEVKARPARI
                     TESEFLWQHNQDPMAVDKLAEGIRKFAIDQEKLEKMIGDLL"
     gene            3702..4289
                     /gene="mog"
                     /locus_tag="ACHOSC_RS19925"
                     /old_locus_tag="pgaptmp_003985"
     CDS             3702..4289
                     /gene="mog"
                     /locus_tag="ACHOSC_RS19925"
                     /old_locus_tag="pgaptmp_003985"
                     /EC_number="2.7.7.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin adenylyltransferase"
                     /protein_id="HGU4549818.1"
                     /translation="MNTLRIGLVSISDRASSGVYQDKGIPALEEWLTSALTTPFELET
                     RLIPDEQAIIEQTLCELVDEMSCHLVLTTGGTGPARRDVTPDATLAVADREMPGFGEQ
                     MRQISLHFVPTAILSRQVGVIRKQALILNLPGQPKSIKETLEGVKDAAGNVVVHGIFA
                     SVPYCIQLLEGPYVETAPEVVAAFRPKSARRDVSE"
     gene            complement(4324..4890)
                     /gene="satP"
                     /locus_tag="ACHOSC_RS19930"
                     /old_locus_tag="pgaptmp_003986"
     CDS             complement(4324..4890)
                     /gene="satP"
                     /locus_tag="ACHOSC_RS19930"
                     /old_locus_tag="pgaptmp_003986"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetate uptake transporter"
                     /protein_id="HGU4549819.1"
                     /translation="MGNTKLANPAPLGLMGFGMTTILLNLHNVGYFALDGIILAMGIF
                     YGGIAQIFAGLLEYKKGNTFGLTAFTSYGSFWLTLVAILLMPKLGLTDAPNAQFLGVY
                     LGLWGVFTLFMFFGTLKGARVLQFVFFSLTVLFALLAIGNIAGNAAIIHFAGWIGLIC
                     GASAIYLAMGEVLNEQFGRTVLPIGESH"
     gene            complement(5039..5752)
                     /gene="msyB"
                     /locus_tag="ACHOSC_RS19935"
                     /old_locus_tag="pgaptmp_003987"
     CDS             complement(5039..5752)
                     /gene="msyB"
                     /locus_tag="ACHOSC_RS19935"
                     /old_locus_tag="pgaptmp_003987"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acidic protein MsyB"
                     /protein_id="HGU4549820.1"
                     /translation="MNVNYLNDSDLDFLQHCSEEQLANFARLLTHNEKGKTRLSSVLM
                     RNELFKSMEGHPEQHRRNWQLIAGELQHFGGDSIANKLRGHGKLYRAILLDVSKRLKL
                     KADKEMSTFEIEQQLLEQFLRNTWKNMDEEHKQEFLHAVDARVNELEELLPLLMKDKL
                     LAKGVSHLLSSQLTRILRTHAAMSVLGHGLLRGAGLGGPVGAALNGVKAVSGSAYRVT
                     IPAVLQIACLRRMVSATQV"
     gene            complement(5778..6182)
                     /locus_tag="ACHOSC_RS19940"
                     /old_locus_tag="pgaptmp_003988"
     CDS             complement(5778..6182)
                     /locus_tag="ACHOSC_RS19940"
                     /old_locus_tag="pgaptmp_003988"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_705972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2541 family protein"
                     /protein_id="HGU4549821.1"
                     /translation="MKSVFTISASLAISLMLCCTAQANDHKILGVIAMPRNETNDLAL
                     KLPVCRIVKRIQLSADHGDLQLSGASVYFKAARSASQSLNIPSEIKEGQTTDWININS
                     DNDNKRCVSKITFSGHTVNSSDMATLKIIGDD"
BASE COUNT         1635 a         1595 c         1595 g         1457 t
ORIGIN      
        1 attctgcaat atattgaatc tgcatgcttt tgtaggccgg ataaggcgtt cacgccgcat
       61 ccggcattga ctgcaaactt aacgctgctc gtagcgttta aacaccagtt cgccattgct
      121 ggaggaagct tcatcaaaga agtaaccttc gctattaaaa ccagtcagtt gctctggttt
      181 ggtcagccga ttttcaataa taaaacgact catcagaccg cgtgctttct tagcgtagaa
      241 gctgattatc ttaaatttgc cgttcttctc atcgaggaac accggcttga taatctcggc
      301 attcaatttc ttcggcttca ccgatttaaa atactcatct gacgccagat taatcaccac
      361 attatcgcct tgtgctgcga gcgcctcgtt cagcttgttg gtgatgatat ctccccagaa
      421 ttgatacaga tctttccctc gggcattctc aagacggatc cccatttcca gacgataagg
      481 ctgcattaaa tcgagcgggc gcagtacgcc atacaagccg gaaagcattc gcaaatgctg
      541 ttgggcaaaa tcgaaatcgt cttcgctgaa ggtttcggcc tgcaagccgg tgtagacatc
      601 acctttaaac gccagaatcg cctggcgggc attctccggc gtgaaatctg gctgccagtc
      661 atgaaagcga gcggcgttga tacccgccag tttgtcgctg atgcgcatca gcgtgctaat
      721 ctgcggaggc gtcagtttcc gcgcttcatg gatcaactgc tgggaattgt ctaacagctc
      781 cggcagcgta tagcgcgtgg tggtcaacgg gctttggtaa tcaagcgttt tcgcaggtga
      841 aataagaatc agcatatcca gtccttgcag gaaatttatg ccgactttag caaaaaaaga
      901 gaatgagttg atcgatagtt gtgattactc ctgcgaaaca tcatcccacg cgtccggaga
      961 aagctggcgg ccgatatccg gataacgcaa cggatcaaac accgggcgca cgccgagttt
     1021 acgctggcgt agataatcac tggcaatggt atgaaccaca ggcgagagca gtaaaatggc
     1081 ggtcaaattg gtaatagcca tgcaggccat tatgatatct gccagttgcc acatcagcgg
     1141 aagacttagc aaggtgccgc cgatgaccgt tgcgaaggtg cagatccgca aacaccagat
     1201 cgctttaggg ttgttcaggc gtaaaaagaa gagattgttt tcggcgtaaa tgtagttggc
     1261 aacgatggag ctgaaggcaa acagaataac cacgagggta acaaactcag caccccagga
     1321 acccattagc acccgcatcg ccttctggat aagctgaata ccttccagcg gcatgtaggt
     1381 tgtgccgtta cccgccagta atatcagcat ggcgcttgcc gtacagatga ccagggtgtc
     1441 gataaaaatg ccaatcatct ggacaatccc ttgcgctgcc ggatgcggag gccaggacgc
     1501 cgctgccgct gccgcgtttg gcgtcgaccc cattcccgcc tcattggaaa acatactgcg
     1561 ctgaaaaccg ttagtaatcg cctggcttaa ggtatatccc gccgcgccgc ctgccgcttc
     1621 ctgccagcca aaagcactct caaaaataga ccaaatgacg tggggaagtt gcccgatatt
     1681 cattacgcaa atcaccaggc tggtcagtac ccagattatc gccatcaacg ggacaaaccc
     1741 ctgcatgagc cgggcgacgc catgaagacc gcgagtgatc gccagcagag caaagacagc
     1801 gagaataata cctgtcacca gcgggggaaa atcaaaagaa aaactcaggg ctcgcgcaac
     1861 ggcgttcgcc tgaattccgc tgaaaattat gccgtaggcg atgagcaaaa agacggcgaa
     1921 cagaacgccc atccagcgca tccccagccc gcgcgccata taccatgccg gtccgccacg
     1981 aaactgccca ttggcatcac gttctttata aagctgtgcg agggaacatt cggcaaacga
     2041 ggtcgccatg ccgataaacg cggcaaccca catccagaag acggctccag gtccaccggc
     2101 ggtaattgcc agcgcaacgc cggccaggtt gccgctaccc acgcgcgccg caagactggt
     2161 acacaatgac tgaaatgagg ttaaaccgcc tggctgtgga tgaatgctat ttttaagact
     2221 tttgccaaac tggcggatgt agcgaaactg cacaaatccg gtgcgaaaag tgaaccaaca
     2281 acctgcgccg aagagcaggt aaatcattac cgatccccaa aggacgctgt taataaagga
     2341 gaaaaaatct ggcatgcata tccctcttat tgccggtcgc gatgactttc ctgtgtaaac
     2401 gttaccaatt gtttaagaag tatatacgct acgaggtact tgataacttc tgcgtagcat
     2461 acatgaggtt ttgtataaaa atggcgggcg atatcaacgc agtgtcagaa atccgaaaca
     2521 gtctcgcctg gcgataaccg tcttgtcggc ggttgcgctg acgttgcgtc gtgatatcat
     2581 cagggcagac cggttacatc cccctaacaa gctgtttaaa gagaaatact atcatgacgg
     2641 acaaattgac ctcccttcgt cagtacacca ccgtagtggc cgacactggg gacatcgcgg
     2701 caatgaagct gtatcaaccg caggatgcca caaccaaccc ttctctcatt cttaacgcag
     2761 cgcagattcc ggaataccgt aagttgattg atgatgctgt cgcctgggcg aaacagcaga
     2821 gcaacgatcg cgcgcagcag atcgtggacg cgaccgacaa actggcagta aatattggtc
     2881 tggaaatcct gaaactggtt ccgggccgta tctcaactga agttgatgcg cgtctttcct
     2941 atgacaccga agcgtcaatt gcgaaagcaa aacgcctgat caaactctac aacgatgctg
     3001 gtattagcaa cgatcgtatt ctgatcaaac tggcttctac ctggcagggt atccgtgctg
     3061 cagaacagct ggaaaaagaa ggcatcaact gtaacctgac cctgctgttc tccttcgctc
     3121 aggctcgtgc ttgtgcggaa gcgggcgtgt tcctgatctc gccgtttgtt ggccgtattc
     3181 ttgactggta caaagcgaat accgataaga aagagtacgc tccggcagaa gatccgggcg
     3241 tggtttctgt atctgaaatc taccagtact acaaagagca cggttatgaa accgtggtta
     3301 tgggcgcaag cttccgtaac atcggcgaaa ttctggaact ggcaggctgc gaccgtctta
     3361 ccatcgcacc tacactgctg aaagagctgg ctgagagcga aggggctatc gaacgtaaac
     3421 tgtcttacac cggcgaagtg aaagcgcgtc cggcgcgtat cactgagtcc gagttcctgt
     3481 ggcagcacaa ccaggatcca atggcagtag ataaactggc ggaaggtatc cgtaagtttg
     3541 ctattgacca ggaaaaactg gaaaaaatga tcggcgatct gctgtaatca ttcttagcgt
     3601 gaccgggaag tcggtcacgc tacctcttct gaagcctgtc tgtcactccc ttcgcagtgt
     3661 atcattctgt ttaacgagac tgtttaaacg gaaaaatctt gatgaatact ttacgtattg
     3721 gcttagtttc catctctgat cgcgcatcca gcggcgttta tcaggataaa ggcatccctg
     3781 cgctggaaga atggctgaca tcggcgctaa ccacgccgtt tgaactggaa acccgcttaa
     3841 tccccgatga gcaggcgatc atcgagcaaa cgttgtgtga gctagtggat gaaatgagtt
     3901 gccatctggt gctcaccacg ggcggaactg gcccggcgcg tcgtgacgta acgcccgatg
     3961 cgacgctggc agtagcggac cgcgagatgc ctggctttgg tgaacagatg cgccagataa
     4021 gcctgcattt tgtaccaact gcgatccttt cgcgtcaggt tggtgtgatt cgcaaacagg
     4081 cgctgatcct taacttaccc ggtcagccga agtctattaa agagacgctg gaaggtgtga
     4141 aggacgctgc gggtaacgtt gtggtacacg gtatttttgc cagcgtaccg tactgcattc
     4201 agttgctgga agggccatac gttgaaacgg caccagaagt ggttgctgca ttcagaccga
     4261 agagtgcaag acgcgacgtt agcgaataaa aaaatccccc cgagcggggg gatctcaaaa
     4321 caattagtgg gattcaccaa tcggcagaac ggtgcgacca aactgctcgt tcagtacttc
     4381 acccatcgcc agatagattg cactggcacc acagatcaga ccaatccagc cggcaaagtg
     4441 gatgattgcg gcgttaccgg caatgttacc gatcgccagc agggcaaaca gcacagtcag
     4501 gctaaagaaa acgaattgca gaacgcgtgc gcctttcagc gtgccgaaga acataaacag
     4561 cgtaaatacg ccccacagac ccaggtagac accaaggaac tgtgcatttg gcgcatcggt
     4621 cagacccagt ttcggcatca gcagaatcgc aaccaacgtc agccagaaag aaccgtaaga
     4681 ggtgaatgcg gttaaaccga aagtgttgcc ttttttgtac tccagcagac cggcaaaaat
     4741 ttgcgcgatg ccgccgtaga aaatgcccat ggcaagaata ataccgtcca gagcgaaata
     4801 acccacgttg tgcaggttaa gcagaatggt ggtcatgccg aagcccatca ggcccagcgg
     4861 tgccggatta gccaacttag tgttgcccat aattcctcaa aaatcatcat cgaatgaatg
     4921 gtgaaataat ttccctgaat aactgtagtg ttttcagggc gcggcataat aatcagccag
     4981 tggggcagtg tctacgatct tttgagggga aaatgaaaat tttccccggt ttccggtatc
     5041 agacctgagt ggcgctaacc atccggcgca ggcaggcgat ttgcagtacg gctggaatcg
     5101 tcacgcgata ggcgctgccg ctgaccgctt taaccccatt tagtgccgca cctacagggc
     5161 ctcccagccc cgcgccgcgc agcaaaccat gcccaagtac gctcattgct gcgtgggtgc
     5221 gtaaaatgcg ggtcagttgg ctggaaagca aatgcgacac gccttttgcc aataatttgt
     5281 ctttcatcag cagcggcagc agctcttcca gctcattcac cctcgcatcg accgcgtgca
     5341 gaaactcctg tttatgttcc tcgtccatat tcttccaggt gttacgcaga aattgttcca
     5401 gtaactgttg ctcaatctca aacgtagaca tctctttgtc ggctttcagc ttcaatcgtt
     5461 ttgaaacatc gagcaaaatg gcccgataca atttaccgtg tccgcgcagt ttgttggcga
     5521 tactatcgcc accaaaatgc tgtaattctc cagcaatcag ctgccagttg cggcgatgtt
     5581 gctcgggatg cccttccata gatttaaaca gttcgttgcg catcagtacg ctggagaggc
     5641 gagttttgcc tttttcatta tgggtgagca atcgggcgaa atttgccaac tgttcctcac
     5701 tgcaatgctg gagaaaatcc agatctgaat cattcaggta attaacattc attttttgtg
     5761 gcttctatat tctggcgtta gtcgtcgccg ataattttca gcgtggccat atccgatgag
     5821 ttcaccgtat gaccggaaaa ggtgattttt gagacgcagc gtttattgtc gttatcgctg
     5881 ttaatgttga tccagtcagt ggtttgccct tcttttattt ctgaaggaat attcaggctc
     5941 tgactggcgc tacgggcggc tttgaaataa accgatgcac cgcttaactg taaatcgcca
     6001 tggtcggcag agagttgtat gcgtttaaca attcggcaaa caggaagttt cagcgccaga
     6061 tcgttggttt cgttacgtgg catcgcaatg acgccgagga ttttatggtc gtttgcctgc
     6121 gccgtgcagc acagcatcag actaatcgcc aggctggcgg aaatcgtaaa aacggatttc
     6181 ataaggattc tcttagtggg gagaggtagg gggatggata cccactagtt tactgctgat
     6241 aaagagaaga ttcaggtgcg taatgttttc tttttattac aa
//
LOCUS       DAZKHF020000248         6271 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2201S31241
            SAMN43267800-rid25106413.denovo.250, whole genome shotgun sequence.
ACCESSION   DAZKHF020000248 DAZKHF020000000
VERSION     DAZKHF020000248.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267800
            Sequence Read Archive: SRR30307263
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6271)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6271)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 6271)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350581.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350581.2
            Genome Coverage       :: 151x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:21:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,652
            CDSs (total)                      :: 5,557
            Genes (coding)                    :: 5,320
            CDSs (with protein)               :: 5,320
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 237
            CDSs (without protein)            :: 237
            Pseudo Genes (ambiguous residues) :: 0 of 237
            Pseudo Genes (frameshifted)       :: 102 of 237
            Pseudo Genes (incomplete)         :: 130 of 237
            Pseudo Genes (internal stop)      :: 52 of 237
            Pseudo Genes (multiple problems)  :: 41 of 237
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6271
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267800-rid25106413.denovo.250"
                     /serotype="O 26:H11"
                     /isolate="2201S31241"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-01"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            <1..288
                     /gene="iraM"
                     /locus_tag="ACHOSC_RS19945"
                     /old_locus_tag="pgaptmp_003989"
     CDS             <1..288
                     /gene="iraM"
                     /locus_tag="ACHOSC_RS19945"
                     /old_locus_tag="pgaptmp_003989"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anti-adapter protein IraM"
                     /protein_id="HGU4549822.1"
                     /translation="SCGISFSAIWGNMKMIIWYQSTIFLPPGSIFTPVKSGIILKDKE
                     YPITIYNIAPFNKDLWSLLKSSQECPPGESKITNKCLHNSCIIKICPYGLK"
     gene            complement(391..1641)
                     /gene="icd"
                     /locus_tag="ACHOSC_RS19950"
                     /old_locus_tag="pgaptmp_003990"
     CDS             complement(391..1641)
                     /gene="icd"
                     /locus_tag="ACHOSC_RS19950"
                     /old_locus_tag="pgaptmp_003990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000444467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004450 - isocitrate dehydrogenase (NADP+)
                     activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent isocitrate dehydrogenase"
                     /protein_id="HGU4549823.1"
                     /translation="MESKVVVPAQGKKITLQNGKLNVPENPIIPYIEGDGIGVDVTPA
                     MLKVVDAAVEKAYKGERKISWMEIYTGEKSTQVYGQDVWLPAETLDLIREYRVAIKGP
                     LTTPVGGGIRSLNVALRQELDLYICLRPVRYYQGTPSPVKHPELTDMVIFRENSEDIY
                     AGIEWKADSADAEKVIKFLREEMGVKKIRFPEHCGIGIKPCSEEGTKRLVRAAIEYAI
                     ANDRDSVTLVHKGNIMKFTEGAFKDWGYQLAREEFGGELIDGGPWLKVKNPNTGKEIV
                     IKDVIADAFLQQILLRPAEYDVIACMNLNGDYISDALAAQVGGIGIAPGANIGDECAL
                     FEATHGTAPKYAGQDKVNPGSIILSAEMMLRHMGWTEAADLIVKGMEGAINAKTVTYD
                     FERLMEGAKLLKCSEFGDAIIKNM"
     gene            1813..2466
                     /gene="rluE"
                     /locus_tag="ACHOSC_RS19955"
                     /old_locus_tag="pgaptmp_003991"
     CDS             1813..2466
                     /gene="rluE"
                     /locus_tag="ACHOSC_RS19955"
                     /old_locus_tag="pgaptmp_003991"
                     /EC_number="5.4.99.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415653.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA pseudouridine(2457) synthase RluE"
                     /protein_id="HGU4549824.1"
                     /translation="MRQFIISENTMQKTSFRNHQVKRFSSQRSTRRKPENQPTRVILF
                     NKPYDVLPQFTDEAGRKTLKEFIPVQGVYAAGRLDRDSEGLLVLTNNGALQARLTQPG
                     KRTGKIYYVQVEGIPTQDALEALRNGVTLNDGPTLPAGAELVDEPAWLWPRNPPIRER
                     KSIPTSWLKITLYEGRNRQVRRMTAHVGFPTLRLIRYAMGDYSLDNLANGEWREVTD"
     gene            2476..2937
                     /gene="nudJ"
                     /locus_tag="ACHOSC_RS19960"
                     /old_locus_tag="pgaptmp_003992"
     CDS             2476..2937
                     /gene="nudJ"
                     /locus_tag="ACHOSC_RS19960"
                     /old_locus_tag="pgaptmp_003992"
                     /EC_number="3.6.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase NudJ"
                     /protein_id="HGU4549825.1"
                     /translation="MFKPHVTVACVVHAEGKFLVVEETINGKALWNQPAGHLEADETL
                     VEAAARELWEETGISAQPQHFIRMHQWIAPDKTPFLRFLFAIELEQICPTQPHDSDID
                     CCRWVSAEEILQASNLRSPLVAESIRCYQSGQRYPLEMIGDFNWPFTKGVI"
     gene            2991..4097
                     /gene="mnmA"
                     /locus_tag="ACHOSC_RS19965"
                     /old_locus_tag="pgaptmp_003993"
                     /gene_synonym="trmU"
     CDS             2991..4097
                     /gene="mnmA"
                     /locus_tag="ACHOSC_RS19965"
                     /old_locus_tag="pgaptmp_003993"
                     /gene_synonym="trmU"
                     /EC_number="2.8.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415651.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004808 - tRNA
                     (5-methylaminomethyl-2-thiouridylate)(34)-
                     methyltransferase activity [Evidence IEA];
                     GO_function: GO:0016783 - sulfurtransferase activity
                     [Evidence IEA];
                     GO_process: GO:0002143 - tRNA wobble position uridine
                     thiolation [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiouridine(34) synthase MnmA"
                     /protein_id="HGU4549826.1"
                     /translation="MSETAKKVIVGMSGGVDSSVSAWLLQQQGYQVEGLFMKNWEEDD
                     GEEYCTAAADLADAQAVCDKLGIELHTVNFAAEYWDNVFELFLAEYKAGRTPNPDILC
                     NKEIKFKAFLEFAAEDLGADYIATGHYVRRADVDGKSRLLRGLDSNKDQSYFLYTLSH
                     EQIAQSLFPVGELEKPQVRKIAEDLGLVTAKKKDSTGICFIGERKFREFLGRYLPAQP
                     GKIITVDGDEIGEHQGLMYHTLGQRKGLGIGGTKEGTEEPWYVVDKDVENNILVVAQG
                     HEHPRLMSVGLIAQQLHWVDREPFTGTMRCTVKTRYRQTDIPCTVKALDDDRIEVIFD
                     EPVAAVTPGQSAVFYNGEVCLGGGIIEQRLPLPV"
     gene            4133..4774
                     /gene="hflD"
                     /locus_tag="ACHOSC_RS19970"
                     /old_locus_tag="pgaptmp_003994"
     CDS             4133..4774
                     /gene="hflD"
                     /locus_tag="ACHOSC_RS19970"
                     /old_locus_tag="pgaptmp_003994"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="high frequency lysogenization protein HflD"
                     /protein_id="HGU4549827.1"
                     /translation="MAKNYYDITLALAGICQSARLVQQLAHQGHCDADALHVSLNSII
                     DMNPSSTLAVFGGSEANLRVGLETLLGVLNASSRQGLNAELTRYTLSLMVLERKLSSA
                     KGALDTLGNRINGLQRQLEHFDLQSETLMSAMAAIYVDVISPLGPRIQVTGSPAVLQS
                     PQVQAKVRATLLAGIRAAVLWHQVGGGRLQLMFSRNRLTTQAKQILAHLTPEL"
     gene            4778..6148
                     /gene="purB"
                     /locus_tag="ACHOSC_RS19975"
                     /old_locus_tag="pgaptmp_003995"
     CDS             4778..6148
                     /gene="purB"
                     /locus_tag="ACHOSC_RS19975"
                     /old_locus_tag="pgaptmp_003995"
                     /EC_number="4.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002950897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004018 - N6-(1,2-dicarboxyethyl)AMP
                     AMP-lyase (fumarate-forming) activity [Evidence IEA];
                     GO_process: GO:0006188 - IMP biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate lyase"
                     /protein_id="HGU4549828.1"
                     /translation="MELSSLTAVSPVDGRYGDKVSALRGIFSEYGLLKFRVQVEVRWL
                     QKLAAHAAIKEVPAFAADAIGYLDAIVANFSEEDAARIKTIERTTNHDVKAVEYFLKE
                     KVAEIPELHAVSEFIHFACTSEDINNLSHALMLKTARDEVILPYWRQLIDGIKDLAVQ
                     YRDIPLLSRTHGQPATPSTIGKEMANVAYRMERQYRQLNQVEILGKINGAVGNYNAHI
                     AAYPEVDWHQFSEEFVTSLGIQWNPYTTQIEPHDYIAELFDCVARFNTILIDFDRDVW
                     GYIALNHFKQKTIAGEIGSSTMPHKVNPIDFENSEGNLGLSNAVLQHLASKLPVSRWQ
                     RDLTDSTVLRNLGVGIGYALIAYQSTLKGVSKLEVNRDHLLDELDHNWEVLAEPIQTV
                     MRRYGIEKPYEKLKELTRGKRVDAEGMKQFIDGLALPEEEKARLKAMTPANYIGRAIT
                     MVDELK"
BASE COUNT         1553 a         1625 c         1550 g         1543 t
ORIGIN      
        1 tcatgcggca tatctttttc agccatatgg ggtaatatga aaatgatcat ctggtatcaa
       61 tctactatat ttctccctcc tggcagtata tttacaccgg ttaaatctgg aattatcctt
      121 aaagataaag aatatcctat tactatttat aacatcgcac cattcaacaa ggatttatgg
      181 agtctactca aaagcagtca agagtgtcct ccaggagaaa gcaaaataac aaataaatgt
      241 ttgcataata gttgcattat aaaaatatgc ccatatgggc tcaagtaaag ggttttatat
      301 aaatatgctc gatatgctat taataatacc tacataaata aaacaacggg cgtgttatac
      361 gcccgttgca atattaaaca catgtagtga ttacatgttc ttgatgatcg catcaccaaa
      421 ttctgaacat ttcagcagtt tagcgccttc catcagacgc tcgaagtcat aggttacggt
      481 cttcgcattg attgcgcctt ccataccttt aacaatcagg tctgcggctt cagtccagcc
      541 catatggcgt aacatcatct cagcggagag aataatagag ccagggttta ctttgtcctg
      601 accggcgtat ttcggcgcag taccgtgggt ggcttcaaac agggcgcatt cgtcaccgat
      661 gtttgctcca ggggcgatac cgataccgcc aacctgcgct gccagagcgt cagaaatgta
      721 gtcaccgttc aggttcatac aggcgataac atcatattca gccggacgca gcaggatctg
      781 ttgcaggaat gcatcagcaa tcacgtcttt aatgacgatc tctttgccgg tgttcgggtt
      841 tttaactttc agccacgggc cgccgtcgat cagttcaccg ccaaactctt cacgcgccag
      901 ctggtagccc cagtctttaa acgcgccttc ggtgaacttc atgatgttgc ctttgtgcac
      961 cagagtcaca gagtcacgat cgttagcaat tgcgtattcg atcgctgcac gaaccagacg
     1021 tttggtgcct tcttccgaac acggcttaat accgatacca caatgttccg ggaagcgaat
     1081 tttcttcacc cccatctctt cacgcaggaa tttaatcact ttctcggcgt cggcagagtc
     1141 tgctttccat tcgatacccg cataaatgtc ttccgagttt tcacggaaga taaccatatc
     1201 ggtcagttca gggtgtttaa ccgggcttgg agtgccttga tagtaacgta ccggacgcag
     1261 gcagatgtag agatccagtt cctggcgcag ggcaacgttc agagagcgaa taccgccacc
     1321 aacaggagtg gtcagcggac ctttaatggc aacgcgatat tcacgaatca gatcaagagt
     1381 ttcagcaggc agccagacgt cctgaccata aacctgtgtg gatttttcac cggtgtaaat
     1441 ttccatccag gagattttac gctcgccttt ataggctttc tcgactgcag cgtcgaccac
     1501 tttcagcatg gctggggtta catctacacc gattccatca ccttcaatgt aagggataat
     1561 cggattttca ggaacgttga gtttgccgtt ttgcagggtg atcttcttgc cttgtgccgg
     1621 aacaactact ttactttcca ttcacctctc cttcgagcgc tactggtttg ctcgtctgcc
     1681 accacgttgc atatgcgttt gcgtcctgcg atacggatgc tttagagcaa ttttttgtta
     1741 atgatttgta attggcttgt caatactacc ctattgtttg ccgtcatgaa agatgcgcgt
     1801 tattaggcta taatgcggca attcataatc tctgaaaata ccatgcaaaa aacttctttt
     1861 agaaatcacc aagttaagcg attcagctcg caacgttcta ccaggcgtaa acctgaaaac
     1921 cagcccacgc gtgtgatcct gttcaataaa ccctacgatg ttcttccgca gttcaccgat
     1981 gaagccggac gcaaaacatt aaaagaattc atcccggttc agggcgttta tgcagcaggt
     2041 cgtcttgacc gcgatagcga agggttactg gtgctgacca ataacggcgc gttgcaggcg
     2101 cgtttaaccc agccgggcaa acgcaccggg aaaatctatt atgtgcaggt ggaaggtatt
     2161 cccacacaag acgcacttga agccttgcgc aatggcgtaa ccttaaatga tggccctacc
     2221 ctgcccgcag gcgcggagct ggttgacgaa cccgcgtggt tatggccgcg gaatccacca
     2281 attcgtgaac gcaaaagtat tcccaccagc tggctgaaga tcaccttata tgaaggacgt
     2341 aatcgccagg tgcgccgcat gaccgcccat gttggtttcc ccacgctgcg actgattcgc
     2401 tatgcgatgg gagattactc tttggataat cttgccaatg gcgaatggcg agaagtgaca
     2461 gattaaggat gcaaaatgtt taaaccgcac gttaccgttg cttgcgtggt gcacgcagaa
     2521 ggcaaatttt tagtcgttga agagacgatt aatggtaaag cgttatggaa ccaacctgcc
     2581 gggcatctgg aagccgatga aaccttagtg gaagccgccg cccgtgaact gtgggaagaa
     2641 accggcatca gcgcgcagcc gcaacacttt attcgtatgc atcagtggat tgcgccagat
     2701 aaaacgccgt ttttgcgttt cctctttgcc attgagcttg agcaaatatg cccgacccag
     2761 cctcatgaca gcgatatcga ctgctgtcgt tgggtcagcg ccgaagaaat tttacaggcg
     2821 tcaaatcttc gttcgccgct ggtggcggaa agtatccgtt gttatcaaag cgggcaacgc
     2881 tatccgctgg agatgattgg cgattttaac tggcctttta caaagggtgt catctaaaag
     2941 gctgcgtgat agaatacgcc gccttgaagt tcaatgtcgt gagtgatcca atgtctgaaa
     3001 ccgcaaaaaa agtaatcgtc ggcatgtccg gcggtgtcga ttcctccgtt tctgcctggc
     3061 tgttgcaaca acagggatat caggtcgaag gcctgtttat gaagaactgg gaagaagacg
     3121 acggtgagga atattgcaca gcggcagcgg atctggctga tgcccaggct gtctgcgaca
     3181 agctcggcat tgaactgcac accgttaact ttgctgccga gtactgggac aacgtcttcg
     3241 aactgttcct tgccgaatat aaagccggtc gcacgccgaa tccggatatt ctgtgcaaca
     3301 aagagatcaa atttaaagcc ttcctcgaat ttgccgccga agatttaggt gccgattata
     3361 tcgctaccgg tcattacgta cgtcgggccg atgtcgatgg caagagccgc ctgctgcgtg
     3421 gtctggacag caataaagac cagagctact tcctttatac gctcagccat gagcagattg
     3481 cgcaaagcct gttcccggtc ggcgaactgg aaaaaccgca ggtgcgtaag attgctgaag
     3541 atcttggtct ggtcaccgcg aagaaaaaag actctaccgg catttgcttc attggcgaac
     3601 gtaaattccg cgagttcctg ggccgttatc tcccggcgca accgggcaaa atcattaccg
     3661 tcgatggcga tgaaattggt gagcaccagg ggctgatgta tcacactctc ggtcagcgta
     3721 aaggtctggg tatcggtggc accaaagaag gtaccgaaga accgtggtat gtggtggaca
     3781 aagacgtcga aaacaacatt ctggttgtcg ctcagggcca tgaacacccg cggctgatgt
     3841 ctgtcgggtt gattgcccag cagttgcact gggtcgatcg cgaaccattc accggcacta
     3901 tgcgttgcac ggtaaaaacc cgctatcgcc agaccgatat cccttgcacc gtcaaggcgc
     3961 tggacgatga tcgcattgaa gtgattttcg atgaaccggt tgccgccgtg acgccgggcc
     4021 agtctgccgt cttctataac ggtgaagtgt gcctcggtgg cggtattatt gagcagcgtc
     4081 tgccgctgcc ggtctgatta ttatctttac ttaaaacaag gaagcagtga acgtggcaaa
     4141 gaattactat gacatcaccc tcgccctggc cggtatttgt cagtcggcac gcctggtgca
     4201 acaactcgct caccaggggc attgtgatgc cgatgcgcta cacgtctcac tcaacagtat
     4261 tattgatatg aaccccagct cgacgctggc ggtttttggc ggtagcgaag ccaacctgcg
     4321 cgtcgggctg gaaaccctgc tcggcgtgct caatgccagc agtcgccagg gcttaaacgc
     4381 cgaattaacc cgctacacac tcagcttgat ggtgcttgag cgcaaactct cctcagcgaa
     4441 aggcgcgctc gacactctgg gcaaccggat caacggcctg caacgccagc tcgaacactt
     4501 cgatttacag tccgaaacgc tgatgagcgc gatggctgct atctatgttg atgtgatcag
     4561 cccgcttggc ccgcgcattc aggtcaccgg ttcccctgct gtactgcaaa gcccacaagt
     4621 gcaggcgaaa gttcgcgcaa ccctgctggc aggcattcgc gccgccgtgc tctggcacca
     4681 ggtcggcggc ggacgtctgc aactgatgtt ttctcgtaat cgcctgacca ctcaggcaaa
     4741 acaaattctt gctcatttaa ccccggagtt gtgatctatg gaattatcct cactgaccgc
     4801 cgtttcccct gtcgatggac gctacggcga taaagtcagc gcgctgcgcg ggattttcag
     4861 cgaatatggt ttgctgaaat tccgtgtaca agttgaagta cgttggctgc aaaaactggc
     4921 cgcgcacgca gcgatcaagg aagttcctgc ttttgctgcc gacgcaatcg gttaccttga
     4981 tgcaattgtc gccaatttca gcgaagaaga tgccgcacgc atcaaaacca tcgagcgtac
     5041 cactaaccac gacgttaaag cggttgagta tttcctgaaa gaaaaagtgg cggagatccc
     5101 ggaactgcac gcggtttctg aattcatcca ctttgcctgt acttcggaag atatcaataa
     5161 cctctcccac gcattaatgc tgaaaaccgc gcgtgatgaa gtgatcctgc cgtactggcg
     5221 tcaactgatt gatggcatta aagatctcgc cgttcagtac cgcgatatcc cgctgctgtc
     5281 tcgtacccac ggtcagccag ccacgccgtc aaccatcggt aaagagatgg caaacgtcgc
     5341 ctaccgtatg gagcgccagt accgccagct taatcaggtg gagatcctcg gcaaaatcaa
     5401 cggcgcggtc ggtaactata acgcccatat cgccgcttac ccggaagttg actggcatca
     5461 gttcagcgaa gagttcgtca cctcgctggg tattcagtgg aacccgtaca ccacccagat
     5521 cgaaccgcac gactacattg ccgaattgtt tgattgcgtt gcgcgcttca acaccattct
     5581 gatcgacttt gaccgtgacg tctggggtta tatcgccctt aaccacttca aacagaaaac
     5641 cattgctggt gagattggtt cttccaccat gccgcataaa gttaacccga ttgacttcga
     5701 aaactccgaa gggaatctgg gcctttccaa cgcggtattg cagcatctgg caagcaaact
     5761 gccggtttcc cgctggcagc gtgacctgac cgactccacc gtgctgcgta acctcggcgt
     5821 gggtatcggt tatgcgctga ttgcgtatca atccaccctg aaaggcgtga gcaaactgga
     5881 agtaaaccgt gaccatctgc tggatgagct ggatcacaac tgggaagtgc tggctgagcc
     5941 aatccagaca gttatgcgtc gctatggcat cgaaaaaccg tacgagaagc tgaaagagct
     6001 gactcgcggt aagcgcgttg acgccgaagg catgaagcag tttatcgatg gtctggcgtt
     6061 gccagaagaa gagaaagccc gcctgaaagc gatgacgccg gctaactata ttggtcgagc
     6121 tatcaccatg gttgatgagc tgaaataaac tcgtatcagt gccggatggc gatgctgtcc
     6181 ggcctgttta ttaaaattat ccactttttc ttttttcact ttacctcccc tccccgctgg
     6241 tttatttaat gtttaccccc ataaacacat a
//
LOCUS       DAZKHF020000249         6262 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2201S31241
            SAMN43267800-rid25106413.denovo.251, whole genome shotgun sequence.
ACCESSION   DAZKHF020000249 DAZKHF020000000
VERSION     DAZKHF020000249.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267800
            Sequence Read Archive: SRR30307263
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6262)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6262)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 6262)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350581.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350581.2
            Genome Coverage       :: 151x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:21:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,652
            CDSs (total)                      :: 5,557
            Genes (coding)                    :: 5,320
            CDSs (with protein)               :: 5,320
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 237
            CDSs (without protein)            :: 237
            Pseudo Genes (ambiguous residues) :: 0 of 237
            Pseudo Genes (frameshifted)       :: 102 of 237
            Pseudo Genes (incomplete)         :: 130 of 237
            Pseudo Genes (internal stop)      :: 52 of 237
            Pseudo Genes (multiple problems)  :: 41 of 237
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6262
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267800-rid25106413.denovo.251"
                     /serotype="O 26:H11"
                     /isolate="2201S31241"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-01"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            52..981
                     /gene="pfkB"
                     /locus_tag="ACHOSC_RS19980"
                     /old_locus_tag="pgaptmp_003996"
     CDS             52..981
                     /gene="pfkB"
                     /locus_tag="ACHOSC_RS19980"
                     /old_locus_tag="pgaptmp_003996"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707391.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016773 - phosphotransferase activity,
                     alcohol group as acceptor [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphofructokinase II"
                     /protein_id="HGU4549829.1"
                     /translation="MVRIYTLTLAPSLDSATITPQIYPEGKLRCTAPVFEPGGGGINV
                     ARAIAHLGGSATAIFPAGGATGEHLVSLLADENVPVATVEAKDWTRQNLHVHVEASGE
                     QYRFVMPGAALNEDEFRQLEEQVLEIESGAILVISGSLPPGVKLEKLTQLISAAQNQG
                     IRCIIDSSGEALSAALAIGNIELVKPNQKELSALVNRELTQPDDVRKAAQEIVNSGKA
                     KRVVVSLGPQGALGVDSENCIQVVPPPVKSQSTVGAGDSMVGAMTLKLAENASLEEMV
                     RFGVAAGSAATLNQGTRLCSHDDTQKIYAYLSR"
     gene            1082..1372
                     /gene="ghoS"
                     /locus_tag="ACHOSC_RS19985"
                     /old_locus_tag="pgaptmp_003997"
     CDS             1082..1372
                     /gene="ghoS"
                     /locus_tag="ACHOSC_RS19985"
                     /old_locus_tag="pgaptmp_003997"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004521 - RNA endonuclease activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type V toxin-antitoxin system endoribonuclease
                     antitoxin GhoS"
                     /protein_id="HGU4549830.1"
                     /translation="MASGDLVRYVITVMLHEDTLTEINELNNYLTRDGFLLTMTDDEG
                     NIHELGTNTFGLISTQSEEEIRELVSGLTQSATGKDPEITITTWEEWNSNRK"
     gene            1478..2338
                     /gene="yniA"
                     /locus_tag="ACHOSC_RS19990"
                     /old_locus_tag="pgaptmp_003998"
     CDS             1478..2338
                     /gene="yniA"
                     /locus_tag="ACHOSC_RS19990"
                     /old_locus_tag="pgaptmp_003998"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructosamine kinase family protein"
                     /protein_id="HGU4549831.1"
                     /translation="MWQAISRLLSEQLGEGEIELRNELPGGEVHAAWHLRYAGHDFFV
                     KCDERELLPGFTAEADQLELLSRSKTVTVPKVWAVGADRDYSFLVMDYLPPRPLDAHS
                     AFILGQQIARLHQWSDQPQFGLDFDNSLSTTPQPNTWQRRWSTFFAEQRIGWQLELAA
                     EKGIAFGNIDAIVEHIQQRLASHQPQPSLLHGDLWSGNCALGPDGPYIFDPACYWGDR
                     ECDLAMLPLHTEQPPQIYDGYQSVSPLPADFLERQPVYQLYTLLNRARLFGGQHLVIA
                     QQSLDRLLAA"
     gene            complement(2379..2915)
                     /gene="yniB"
                     /locus_tag="ACHOSC_RS19995"
                     /old_locus_tag="pgaptmp_003999"
     CDS             complement(2379..2915)
                     /gene="yniB"
                     /locus_tag="ACHOSC_RS19995"
                     /old_locus_tag="pgaptmp_003999"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YniB family protein"
                     /protein_id="HGU4549832.1"
                     /translation="MTYQQAGRIAVLKRILGWVIFIPALISTLISLLKFMNTRQENQE
                     GINAVMLDFTHVMIDMMQANTPFLNLFWYNSPTPNFNGGVNVMFWVIFILIFVGLALQ
                     DSGARMSRQARFLREGVEDQLILEKAKGEEGLTREQIESRIVVPHHTIFLQFFSLYIL
                     PVICIAAGYVFFSLLGFI"
     gene            3062..3730
                     /gene="hxpB"
                     /locus_tag="ACHOSC_RS20000"
                     /old_locus_tag="pgaptmp_004000"
     CDS             3062..3730
                     /gene="hxpB"
                     /locus_tag="ACHOSC_RS20000"
                     /old_locus_tag="pgaptmp_004000"
                     /EC_number="3.1.3.22"
                     /EC_number="3.1.3.50"
                     /EC_number="3.1.3.68"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hexitol phosphatase HxpB"
                     /protein_id="HGU4549833.1"
                     /translation="MSTPRQILAAIFDMDGLLIDSEPLWDRAELDVMASLGVDISRRN
                     ELPDTLGLRIDMVVDLWYARQPWNGPSRQEVVERVIARAISLVEETRPLLPGVREAVA
                     LCKEQGLLVGLASASPLHMLEKVLTMFDLRDSFDALASAEKLPYSKPHPQVYLDCAAK
                     LGVDPLTCVALEDSVNGMIASKAARMRSIVVPAPEAQNDPRFVLANVKLSSLTELTAK
                     DLLG"
     gene            3893..4483
                     /gene="ydjM"
                     /locus_tag="ACHOSC_RS20005"
                     /old_locus_tag="pgaptmp_004001"
     CDS             3893..4483
                     /gene="ydjM"
                     /locus_tag="ACHOSC_RS20005"
                     /old_locus_tag="pgaptmp_004001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310461.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HGU4549834.1"
                     /translation="MTAEGHLLFSIACAVFAKNAELTPVLAQGDWWHIVPSAILTCLL
                     PDIDHPKSFLGQRLKWISKPIARAFGHRGFTHSLLAVFALLATFYLKVPESWFIPADA
                     LQGMVLGYLSHILADMLTPAGVPLLWPCRWRFRLPILVPQKGNQLERFICMALFVWSV
                     WMPHSLPENSAVRWSSQMINTLQIQFHRLIKHQVEY"
     gene            4616..6007
                     /gene="tcyP"
                     /locus_tag="ACHOSC_RS20010"
                     /old_locus_tag="pgaptmp_004002"
     CDS             4616..6007
                     /gene="tcyP"
                     /locus_tag="ACHOSC_RS20010"
                     /old_locus_tag="pgaptmp_004002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005120792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cystine/sulfocysteine:cation symporter"
                     /protein_id="HGU4549835.1"
                     /translation="MNFPLIANIVVFVVLLFALAQTRHKQWSLAKKVLVGLVMGVVFG
                     LALHTIYGSDSQVLKDSVQWFNIVGNGYVQLLQMIVMPLVFASILSAVARLHNASQLG
                     KISFLTIGTLLFTTLIAALVGVLVTNLFGLTAEGLVQGGAETARLNAIESNYVGKVSD
                     LSVPQLVLSFIPKNPFADLTGANPTSIISVVIFAAFLGVAALKLLKDDAPKGERVLTA
                     IDTLQSWVMKLVRLVMQLTPYGVLALMTKVVAGSNLQDIIKLGSFVVASYLGLLIMFA
                     VHGILLGINGVSPLKYFRKVWPVLTFAFTSRSSAASIPLNVEAQTRRLGVPESIASFA
                     ASFGATIGQNGCAGLYPAMLAVMVAPTVGINPLDPMWIATLVGIVTVSSAGVAGVGGG
                     ATFAALIVLPAMGLPVTLVALLISVEPLIDMGRTALNVSGSMTAGTLTSQWLKQTDKA
                     ILDSEDDAELAHR"
     gene            complement(6011..>6262)
                     /gene="ydjO"
                     /locus_tag="ACHOSC_RS20015"
                     /old_locus_tag="pgaptmp_004003"
     CDS             complement(6011..>6262)
                     /gene="ydjO"
                     /locus_tag="ACHOSC_RS20015"
                     /old_locus_tag="pgaptmp_004003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416244.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YdjO"
                     /protein_id="HGU4549836.1"
                     /translation="LPGMGSGVLEDIMSIPDSSLRKRLGYEVLSFSLQAHSLSQECID
                     KLDIFFADDLFKYESVCIAAMEHLKSKATAPIQNGPLPA"
BASE COUNT         1532 a         1553 c         1573 g         1604 t
ORIGIN      
        1 cactttcggc tgattcggtg ccagactgaa atcagcctat aggaggaaat gatggtacgt
       61 atctatacgt tgacacttgc gccctctctc gatagcgcaa caattacccc gcaaatttat
      121 cccgaaggaa aactgcgctg taccgcaccg gtgttcgaac ccgggggcgg cggcatcaac
      181 gtcgcccgcg ccattgccca tcttggaggc agtgccacag cgatcttccc ggcgggtggc
      241 gcgaccggcg aacacctggt ttctctgttg gcggatgaaa atgtccccgt cgctactgta
      301 gaagccaaag actggacccg gcagaattta cacgtacatg tggaagcaag cggtgagcag
      361 tatcgttttg ttatgccagg cgcggcatta aatgaagatg agtttcgcca gcttgaagag
      421 caagttctgg aaattgaatc cggggccatc ctggtcataa gcggaagcct gccgccaggt
      481 gtgaagctgg aaaaattaac ccaactgatt tccgctgcgc aaaaccaagg gatccgctgc
      541 atcatcgaca gttctggcga agcgttaagt gcagcactgg cgattggtaa catcgagttg
      601 gttaagccta accaaaaaga actcagtgcg ctggtgaatc gcgaactcac ccagccagac
      661 gatgtccgca aagccgcgca ggaaatcgtt aatagcggca aggccaaacg ggttgtcgtt
      721 tcccttggtc cacaaggagc gctgggtgtt gatagtgaaa actgcattca ggtggtgcca
      781 ccaccggtga aaagccagag taccgttggc gctggtgaca gcatggtcgg cgcgatgaca
      841 ctgaaactgg cagaaaatgc ctctcttgaa gagatggtcc gttttggcgt agctgcgggg
      901 agtgcagcca cactcaatca gggaacacgt ctgtgctccc atgacgatac gcaaaaaatt
      961 tacgcttacc tttcccgcta acaaaaacat tcccccagca ttgggggaat caccaccaac
     1021 ctgtcggcaa cgcgtttctc cgactatgct caaaagtcat gtgataacaa aggggtgaac
     1081 tatggccagt ggcgatcttg tccgttatgt cataaccgtc atgttgcatg aggatacatt
     1141 gactgaaatt aacgagttga ataattacct gactcgcgac ggttttttgc tcaccatgac
     1201 ggatgatgag ggaaatatcc atgagctggg gactaacact tttggactta tcagtaccca
     1261 aagtgaagaa gaaattagag aactggtttc ggggcttacc caaagtgcaa ccggcaaaga
     1321 tcctgaaatc accatcacga cctgggagga atggaatagc aacagaaaat aaatggtttt
     1381 tgggcaataa tcagtctgtg gtgtgcgtta gctcgtgttt ttacaccgca ttcttgcgct
     1441 aaccttatga tctggcagac aacatgggag agacatcatg tggcaggcaa tcagtcgtct
     1501 tttgagcgag cagttaggtg aaggcgaaat cgaactgcgt aatgaactgc ctggcggaga
     1561 agtccatgcc gcatggcatt tgcgctatgc aggacatgac tttttcgtca aatgtgatga
     1621 aagggaactg cttcccggtt ttaccgccga agccgaccaa ctggagttac tgtcgcgtag
     1681 taaaaccgtc accgtgccta aggtttgggc agtaggcgct gaccgtgact acagttttct
     1741 ggtgatggat tatctcccac ctcgtccgct ggatgcgcat agcgcattta ttcttggtca
     1801 gcaaattgcg cgtttacatc aatggagtga ccaaccacaa tttggcctcg attttgataa
     1861 ctcgctctcc acaactccac agcccaacac ctggcaacgt cgctggtcaa cgttttttgc
     1921 tgaacaacgg attggctggc agttggaact ggcagcagag aaagggatcg ctttcggcaa
     1981 tatcgacgcc atcgtcgagc atattcagca gcgtctggcc tcgcatcaac cgcagccttc
     2041 tctgttgcac ggcgatttat ggtccggcaa ctgtgcgctg ggtccggatg gcccgtacat
     2101 tttcgaccct gcctgctact ggggtgaccg agagtgcgac ctggcgatgt taccgctgca
     2161 tactgaacag ccgccacaaa tctatgacgg ctatcagtca gtatccccgc tacctgccga
     2221 tttccttgaa cgtcaaccgg tttaccaact ctacacgctg ttaaatcgtg caagattatt
     2281 tggcggtcag catttggtta ttgctcagca gtcattggat agattattag cagcatgata
     2341 tgggttgagg ataatggccg ctccgtgcgg ccttttgatt aaataaaccc aagcagagaa
     2401 aagaacacat aacctgcagc aatacaaata accggcagga tatatagtga gaaaaactgc
     2461 aggaaaatag tatggtgcgg aacaacaata cgggactcaa tttgttcacg cgttagcccc
     2521 tcttcccctt tggctttttc cagaatgagt tgatcttcaa caccttcccg caagaagcgc
     2581 gcctggcgac tcatccgggc accggaatcc tgcaacgcca gtccgacaaa aatcaggatg
     2641 aaaatcaccc agaacataac gttcacgcca ccattaaaat tgggcgtcgg ggagttatac
     2701 cagaacagat tcaaaaaagg cgtattcgcc tgcatcatat cgatcatgac atgagtaaag
     2761 tcgagcatga ccgcattaat gccttcctgg ttttcctgcc gggtattcat aaatttcagc
     2821 aaagaaatca atgtagagat caatgcaggt ataaaaatca cccaacccaa aatccttttc
     2881 aaaacagcaa tgcgtccagc ttgttgatac gtcatgagtt ctccttgatt aagacgcgtc
     2941 gtttcactta gtttacctgt agatatctgt tttcgcccat tctttaaagg cgatatgata
     3001 ggcgcttaat cataagcacg gcttaatacc ttacacataa tgctctaaag gagaggttgt
     3061 aatgtcaacc ccgcgtcaga ttcttgctgc aatttttgat atggatggat tacttatcga
     3121 ctcagaacct ttatgggatc gagccgaact ggatgtgatg gcaagcctgg gggtggatat
     3181 ctcccgtcgt aacgagctgc cggacacctt aggtttacgc atcgatatgg tggtcgatct
     3241 ttggtacgcc cggcaaccgt ggaatgggcc aagccgtcag gaagtagtag aacgggttat
     3301 tgcccgtgcc atttcactgg ttgaagagac acgtccatta ttaccaggcg tgcgcgaagc
     3361 cgttgcgtta tgcaaagaac aaggtttatt ggtgggactg gcctccgcgt caccactaca
     3421 tatgctggaa aaagtgttga ccatgtttga cttacgcgac agtttcgatg ccctcgcctc
     3481 ggctgaaaaa ctgccttaca gcaagccgca tccgcaagta tatctcgact gcgcagcaaa
     3541 actgggcgtt gacccactga cctgcgtagc gctggaagat tcagtaaatg gcatgatcgc
     3601 ctctaaagca gcccgcatgc gttccattgt cgttcctgcg ccagaagcgc aaaatgatcc
     3661 acgttttgta ttagcaaacg tcaaactctc atcgctgaca gaactcaccg caaaagacct
     3721 tctcggttaa tgaccagggg cagtgatctc gctgcccctg gttctttatc tgaatttgca
     3781 ttcaactgac ggattaatcg tcaatttaag agaaagagtt acaccgtcac cacttccgtg
     3841 cactgtataa aaatcctata ctgtacgtat cgacagttta gtgagtttta tcatgacggc
     3901 ggaaggtcac cttctctttt ctattgcttg tgcggtattt gccaaaaatg ccgagctgac
     3961 gcccgtgctg gcacagggtg actggtggca tattgtccct tccgcaatcc tgacgtgttt
     4021 gttaccggac atcgatcacc caaagtcgtt tcttgggcag cgattaaagt ggatatcaaa
     4081 accgatcgcc cgcgcttttg ggcatcgtgg ttttacccac agtctgctgg cggtatttgc
     4141 gctgctggca accttttacc ttaaggttcc tgaaagctgg ttcattccgg ctgatgcgct
     4201 acaaggaatg gtgctgggtt atttaagcca catacttgcc gatatgctga cacccgccgg
     4261 tgttcccctg ctctggccat gtcgctggcg tttccgcttg cctatcctgg ttccccaaaa
     4321 gggcaaccaa ctggaacgtt ttatctgcat ggcattattt gtctggtcgg tatggatgcc
     4381 ccattcatta cccgaaaaca gcgcggttcg ttggtcatcg caaatgatca ataccttgca
     4441 gatccagttt catcggctta ttaagcatca ggttgaatac taaaaaggca aaaatcacct
     4501 ttctggaata agcaattcca tttgaatata agagccagct cacagttctg ttaatcttgc
     4561 gccaacacta tgactgctac gcagtgatag aaataataag atcaggagaa cggggatgaa
     4621 ctttccatta attgcgaaca tcgtggtgtt cgttgtactg ctgtttgcgc tggctcagac
     4681 ccgccacaaa cagtggagtc tggcgaaaaa agtgctggtg ggtctggtga tgggtgtggt
     4741 ttttggcctt gccctgcata ccatttatgg ttctgacagc caggtactta aagattctgt
     4801 acagtggttt aacatcgttg gtaacggcta tgttcaactg ctgcaaatga tcgttatgcc
     4861 gttagtcttc gcctctattc tgagcgcggt tgcccgtctg cataacgcat ctcagttagg
     4921 caaaatcagt tttctgacca tcggtacgct tttgtttacc acactgattg cggcgctggt
     4981 cggtgtgctg gtcaccaacc tgtttggttt gacggctgaa ggtctggttc agggtggtgc
     5041 agaaactgca cgtctgaacg ccatcgaaag taactatgtt ggtaaagtct ctgacctgag
     5101 cgttccgcag ctggtcttgt cctttatccc gaaaaacccg tttgccgatc tgaccggagc
     5161 caatccgacg tcaattatca gcgtggtaat ttttgccgca ttcctcggtg tagctgcgct
     5221 gaaactgctg aaggatgatg cgccgaaagg tgaacgcgtc ttaaccgcta tcgataccct
     5281 gcaaagctgg gtgatgaaac tggttcgcct ggtcatgcag ttgacccctt acggcgttct
     5341 ggcactaatg accaaagtgg ttgcaggttc taacctgcaa gacatcatca aactgggaag
     5401 tttcgttgtc gcgtcctacc tcggtctgct gattatgttt gcagtgcatg gcattctgct
     5461 gggcattaat ggcgtgagtc cgctgaagta cttccgtaag gtatggcctg tgctgacgtt
     5521 tgcctttacc agccgttcca gtgctgcgtc tatcccactg aatgtggaag cacaaacgcg
     5581 tcgtctgggc gttcctgaat ccatcgccag tttcgccgcc tctttcggtg caaccattgg
     5641 tcagaacggc tgcgccggtt tgtatccggc aatgctggcg gtgatggttg cgcctacggt
     5701 tggcattaac ccgctggacc cgatgtggat tgcgacgctg gtcggtattg ttaccgttag
     5761 ttccgcaggc gttgccggtg tcggtggtgg tgcaactttc gccgcactga ttgtgctgcc
     5821 tgcgatgggc ctgccagtaa ccctggtggc gctgttaatc tccgttgaac cgcttatcga
     5881 catgggccgt acggcgctaa acgttagtgg ctcgatgaca gctggcacgc tgaccagcca
     5941 gtggctgaag caaaccgata aagccattct ggatagcgaa gacgacgccg aactggcaca
     6001 ccgttaattc ttatgctggc aacggtccgt tttgtatagg ggccgttgct ttacttttta
     6061 aatgttccat cgcggcgatg catactgact catatttaaa cagatcgtca gcgaaaaaaa
     6121 tgtccaactt atcaatacat tcctggctca atgaatgtgc ctgtagtgag aagcttaaaa
     6181 cctcataccc gagacgtttt ctaagtgaac tatctggaat actcataata tcttcaagca
     6241 caccacttcc cattcccgga ag
//
LOCUS       DAZKHF020000250         6257 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2201S31241
            SAMN43267800-rid25106413.denovo.252, whole genome shotgun sequence.
ACCESSION   DAZKHF020000250 DAZKHF020000000
VERSION     DAZKHF020000250.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267800
            Sequence Read Archive: SRR30307263
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6257)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6257)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 6257)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350581.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350581.2
            Genome Coverage       :: 151x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:21:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,652
            CDSs (total)                      :: 5,557
            Genes (coding)                    :: 5,320
            CDSs (with protein)               :: 5,320
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 237
            CDSs (without protein)            :: 237
            Pseudo Genes (ambiguous residues) :: 0 of 237
            Pseudo Genes (frameshifted)       :: 102 of 237
            Pseudo Genes (incomplete)         :: 130 of 237
            Pseudo Genes (internal stop)      :: 52 of 237
            Pseudo Genes (multiple problems)  :: 41 of 237
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6257
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267800-rid25106413.denovo.252"
                     /serotype="O 26:H11"
                     /isolate="2201S31241"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-01"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            complement(<1..72)
                     /locus_tag="ACHOSC_RS20020"
                     /old_locus_tag="pgaptmp_004004"
     CDS             complement(<1..72)
                     /locus_tag="ACHOSC_RS20020"
                     /old_locus_tag="pgaptmp_004004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4549837.1"
                     /translation="MREQIKQDIDLIEILFYLKKKVRV"
     gene            complement(285..1955)
                     /gene="betA"
                     /locus_tag="ACHOSC_RS20025"
                     /old_locus_tag="pgaptmp_004005"
     CDS             complement(285..1955)
                     /gene="betA"
                     /locus_tag="ACHOSC_RS20025"
                     /old_locus_tag="pgaptmp_004005"
                     /EC_number="1.1.99.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008812 - choline dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0019285 - glycine betaine biosynthetic
                     process from choline [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="choline dehydrogenase"
                     /protein_id="HGU4549838.1"
                     /translation="MQFDYIIIGAGSAGNVLATRLTEDPNTSVLLLEAGGPDYRFDFR
                     TQMPAALAFPLQGKRYNWAYETEPEPFMNNRRMECGRGKGLGGSSLINGMCYIRGNAL
                     DLDNWAQEPGLENWSYLDCLPYYRKAETRDVGENDYHGGDGPVSVTTSKPGVNPLFEA
                     MIEAGVQAGYPRTDDLNGYQQEGFGPMDRTVTPQGRRASTARGYLDQAKSRPNLTIRI
                     HAMTDHIIFDGKRAVGVEWLEGDSTIPTRATANKEVLLCAGAIASPQILQRSGVGNAE
                     LLAEFDIPLVHELPGVGENLQDHLEMYLQYECKEPVSLYPALQWWNQPKIGAEWLFGG
                     TGVGASNHFEAGGFIRSREEFAWPNIQYHFLPVAINYNGSNAVKEHGFQCHVGSMRSP
                     SRGHVRIKSRDPHQHPAILFNYMSHEQDWQEFRDAIRITREIMHQPALDQYRGREISP
                     GTECQTDEQLDEFVRNHAETAFHPCGTCKMGYDEMSVVDGEGRVHGLEGLRVVDASIM
                     PQIITGNLNATTIMIGEKIADMIRGQEALPRSTAGYFVANGMPVRAKK"
     gene            complement(1969..3441)
                     /gene="betB"
                     /locus_tag="ACHOSC_RS20030"
                     /old_locus_tag="pgaptmp_004006"
     CDS             complement(1969..3441)
                     /gene="betB"
                     /locus_tag="ACHOSC_RS20030"
                     /old_locus_tag="pgaptmp_004006"
                     /EC_number="1.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008802 - betaine-aldehyde dehydrogenase
                     (NAD+) activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0019285 - glycine betaine biosynthetic
                     process from choline [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="betaine-aldehyde dehydrogenase"
                     /protein_id="HGU4549839.1"
                     /translation="MSRMAEQQLYIHGGYTSATSGRTFETINPANGNVLATVQAAGRE
                     DVDRAVKSAQQGQKIWAAMTAMERSRILRRAVDILRERNDELAKLETLDTGKAYSETS
                     TVDIVTGADVLEYYAGLIPALEGSQIPLRETSFVYTRREPLGVVAGIGAWNYPIQIAL
                     WKSAPALAAGNAMIFKPSEVTPLTALKLAEIYSEAGLPDGVFNVLPGGGAETGQYLTE
                     HPGIAKVSFTGGVASGKKVMANSAASSLKEVTMELGGKSPLIVFDDADLDLAADIAMM
                     ANFFSSGQVCTNGTRVFVPAKCKAAFEQKILARVERIRAGDVFDPQTNFSPLVSFPHR
                     DNVLRYIAKGKEEGARVLCGGDVLKGDGFDNGAWVAPTVFTDCRDEMTIVREEIFGPV
                     MSLLTYESEDEVIRRANDTDYGLAAGIVTADLNRAHRVIHQLEAGICWINTWGESPAE
                     MPVGGYKHSGIGRENGVMTLQSYTQVKSIQVEMAKFQSIF"
     gene            complement(3455..4042)
                     /gene="betI"
                     /locus_tag="ACHOSC_RS20035"
                     /old_locus_tag="pgaptmp_004007"
     CDS             complement(3455..4042)
                     /gene="betI"
                     /locus_tag="ACHOSC_RS20035"
                     /old_locus_tag="pgaptmp_004007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308386.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator BetI"
                     /protein_id="HGU4549840.1"
                     /translation="MPKLGMQSIRRRQLIDATLEAINEVGMHDATIAQIARRAGVSTG
                     IISHYFRDKNGLLEATMRDITSQLRDAVLNRLHALPQGSAEQRLQAIVGGNFDETQVS
                     SAAMKAWLAFWASSMHQPMLYRLQQVSSRRLLSNLVSEFRRELPRQQAQEAGYGLAAL
                     IDGLWLRAALSGKPLDKPLAHSLTRHFITQHLPTD"
     gene            4171..6204
                     /gene="betT"
                     /locus_tag="ACHOSC_RS20040"
                     /old_locus_tag="pgaptmp_004008"
     CDS             4171..6204
                     /gene="betT"
                     /locus_tag="ACHOSC_RS20040"
                     /old_locus_tag="pgaptmp_004008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="choline BCCT transporter BetT"
                     /protein_id="HGU4549841.1"
                     /translation="MTDLSHSREKDKINPVVFYTSAGLILLFSLTTILFRDFSALWIG
                     RTLDWVSKTFGWYYLLAATLYIVFVVCIACSRFGSVKLGPEQSKPEFSLLSWAAMLFA
                     AGIGIDLMFFSVAEPVTQYMQPPEGAGQTIEAARQAMVWTLFHYGLTGWSMYALMGMA
                     LGYFSYRYNLPLTIRSALYPIFGKRINGPIGHSVDIAAVIGTIFGIATTLGIGVVQLN
                     YGLSVLFDIPDSMAAKAALIALSVIIATISVTSGVDKGIRVLSELNVALALGLILFVL
                     FMGDTSFLLNALVLNVGDYVNRFMGMTLNSFAFDRPVEWMNNWTLFFWAWWVAWSPFV
                     GLFLARISRGRTIRQFVLGTLIIPFTFTLLWLSVFGNSALYEIIHGGAAFAEEAMVHP
                     ERGFYSLLAQYPAFTFSASVATITGLLFYVTSADSGALVLGNFTSQLKDINSDAPGWL
                     RVFWSVAIGLLTLGMLMTNGISALQNTTVIMGLPFSFVIFFVMAGLYKSLKVEDYRRE
                     SANRDTAPRPLGLQDRLSWKKRLSRLMNYPGTRYTKQMMETVCYPAMEEVAQELRLRG
                     AYVELKSLPPEEGQQLGHLDLLVHMGEEQNFVYQIWPQQYSVPGFTYRARSGKSTYYR
                     LETFLLEGSQGNDLMDYSKEQVITDILDQYERHLNFIHLHREAPGHSVMFPDA"
BASE COUNT         1281 a         1730 c         1732 g         1514 t
ORIGIN      
        1 aacacgaacc tttttcttca gataaaataa aatctcaatc agatcgatat cctgtttgat
       61 ttgttcacgc ataatatatc cagaaaataa aatctgtcgc agataaggtt gtattaatag
      121 tctgtatcag gaatgttcgg gttaaatatc agcaaaaagc cagcatcatg gatactggat
      181 atgaagcatg agagttacct cggtgtttat ataaggattc ggtccccctc tctggaacgg
      241 taactctcaa tctgatcggt tcctgcgtta gttcacatca cgactcattt tttcgctctc
      301 accggcatcc catttgccac aaaatatccc gccgtgctcc tcggcagcgc ttcctgtcca
      361 cgaatcatat ccgctatttt ctcgccaatc ataattgtcg tggcgttcaa attcccggtg
      421 ataatctgcg gcataatcga cgcatccacc acacgcaggc cttctaaccc gtgtacgcgg
      481 ccttcgccgt caaccacgga catctcgtcg taacccattt tgcaggtacc gcacggatgg
      541 aaggcggttt cggcgtggtt acgcacgaac tcatcgagct gttcatccgt ctgacattcc
      601 gtgccggggc tgatttcgcg gccacgatac tgatccagcg cgggttgatg catgatctcg
      661 cgggtgatgc gaattgcgtc gcggaactcc tgccagtcct gctcgtgcga catgtagtta
      721 aacagaatcg ccggatgctg gtgcgggtcg cgggatttaa tccgcacatg cccacggctt
      781 ggcgagcgca ttgagccgac gtggcactgg aaaccgtgct ctttcactgc attcgagccg
      841 ttatagttaa tcgctactgg caggaaatgg tactgaatat tcggccacgc aaattcctca
      901 cggctgcgaa taaatccacc tgcttcaaag tggttgctgg caccaacgcc agtgccgcca
      961 aacagccact ccgcaccgat tttcggctgg ttccaccact gcagggcagg gtagagggaa
     1021 accggttctt tgcactcata ttgcagatac atctccagat gatcctgaag attttcgccg
     1081 acgccgggta attcatgcac cagcggaata tcaaactccg ccagcagttc agcgttgccg
     1141 acgccggagc gttgcaggat ctgcggtgag gcaatcgcgc ctgcacataa cagcacttct
     1201 ttgttggccg ttgcgcgggt tgggatggtg ctgtcgcctt ccagccattc gacgcccacc
     1261 gcgcgtttgc cgtcaaaaat gatgtgatcg gtcatagcgt gaatacgaat ggtcaggtta
     1321 gggcgcgatt tggcctgatc gagataaccg cgcgcggtac tggcgcgacg gccctgcggc
     1381 gtgacggtgc gatccatcgg accaaaacct tcctgctgat aaccgttgag atcgtccgtg
     1441 cgcgggtagc ccgcctgcac gcccgcttca atcatcgctt caaacagcgg attgacgccg
     1501 ggtttggagg tagtgacgct caccgggcca tcgccaccgt ggtagtcgtt ctcgcccaca
     1561 tcgcgagtct cggccttgcg gtagtagggc aggcagtcga gataactcca gttctccaga
     1621 ccgggttctt gcgcccagtt atcgagatcc agcgcattgc cacggatgta gcacatgccg
     1681 ttgatcagcg acgatccacc caggccttta ccgcgtccgc actccatgcg gcggttattc
     1741 ataaacggtt caggctctgt ttcataggcc cagttgtagc gtttgccctg tagtgggaac
     1801 gccagcgctg cgggcatctg ggtgcggaag tcaaagcgat agtccgggcc gcccgcttca
     1861 agcagcagca cggaggtatt cggatcttca gtcagacggg tagcgagaac gttgccggct
     1921 gagccggcac caataatgat gtagtcaaat tgcaaataaa cctcctggtt agaatatgga
     1981 ctggaattta gccatctcaa cctggatgga cttcacctgg gtgtaactct ggagcgtcat
     2041 cacgccgttc tcgcgaccaa tgccggagtg tttgtagccg ccaacgggca tctctgccgg
     2101 ggattcgccc caggtgttga tccagcaaat acccgcttcc agctgatgaa tgacgcgatg
     2161 cgcgcggttc aggtccgccg tcacgatgcc cgccgccagg ccgtagtcgg tatcattggc
     2221 gcggcgaatc acttcgtcct cagattcata agtgagaagc gacatcaccg gaccgaagat
     2281 ctcttcgcgc acgatggtca tttcgtcgcg gcaatcggtg aacacggtcg gtgcaaccca
     2341 tgcgccgtta tcgaagccat cgcctttcag tacatcgccg ccgcacagta cgcgcgcgcc
     2401 ttcctctttg cctttggcga tatagcgcag cacgttatcg cgatgcggga agctgaccag
     2461 cgggctgaag ttagtttgcg gatcgaaaac gtcgcccgcg cgaatgcgct caacgcgcgc
     2521 cagaattttc tgctcaaatg cggctttgca tttcgccgga acgaagacgc gggtgccgtt
     2581 ggtacacacc tgaccggagc tgaagaagtt cgccatcatg gcgatatcgg cggcgagatc
     2641 gagatccgca tcgtcgaaaa cgatcagcgg tgatttaccg cccagttcca tggtcacttc
     2701 tttcagggaa gaggccgccg agttagccat cacttttttg ccgctggcga caccgccggt
     2761 aaatgacact ttggcaatgc ccggatgctc ggtcagatat tgcccggtct ccgcgccccc
     2821 ccccggcaac acgttaaata cgccgtccgg caggcccgct tcgctgtaaa tttcagccag
     2881 ctttaacgcg gtaagcgggg tgacttcgct cggtttgaaa atcattgcgt tgcctgccgc
     2941 cagcgccggg gcggatttcc acagggcaat ctggatcggg tagttccatg cgccaatccc
     3001 cgccaccaca cccagcggtt cgcggcgggt ataaacaaat gacgtctcac gcaacgggat
     3061 ctggctgcct tccagcgccg ggatcagccc ggcgtagtac tccagcacgt ccgcaccggt
     3121 aacgatatcg acggttgagg tttccgaata tgcttttccg gtatcgaggg tttccagttt
     3181 tgcgagttcg tcattgcgtt cacgcagaat atcaacggcc cgacgcagaa tacgcgagcg
     3241 ctccatagcg gtcatcgccg cccagatttt ttgcccctgt tgggcactct tcacggcgcg
     3301 atcgacatcc tcacgcccgg cggcctgcac ggtcgccagc acgttaccgt tggccgggtt
     3361 aatggtctcg aaggtgcgac cgctggtggc ggaggtataa ccaccatgta tataaagctg
     3421 ctgttctgcc attcgggaca tcacgtctcc tcggttaatc ggtgggtaaa tgctgagtaa
     3481 taaagtgacg ggtcagcgag tgtgcgagag gtttatccag cggtttgccg ctcagtgccg
     3541 cgcgcagcca taatccgtca atcagcgcgg caaggccgta gcccgcttcc tgtgcctgtt
     3601 ggcgcggtaa ctcgcggcga aattcgctca ccagattcga caataagcgg cggctactga
     3661 cctgctgtaa acgatagagc atcggctgat gcatactgct ggcccagaac gccagccagg
     3721 ctttcatcgc cgcactgctc acctgcgttt catcgaagtt tccgccaaca atcgcctgta
     3781 atcgctgctc tgcactgccc tgcggaagtg catgtaatcg attcaaaacc gcgtcacgca
     3841 gctgactggt gatatcgcgc atggttgctt ccagcagacc atttttgtcc ctgaaatagt
     3901 ggctgatgat ccccgtagaa acgcctgcac ggcgggcgat ctgcgcgatc gttgcatcgt
     3961 gcatgcccac ttcatttatt gcttccagtg tggcgtcgat cagttgtctg cgccggatcg
     4021 actgcatccc caatttgggc attttcgcca ctccattcat cagcgttgtt tatctattaa
     4081 agcggttatt gattggacgt tcaatataaa atgtgtctta attgttacga atttgatttt
     4141 aaatggtaac aataacagtg gggatactgg atgacagacc tttcacacag cagggaaaag
     4201 gacaaaatca atccggtggt gttttacacc tccgccggac tgattttgtt gttttccctg
     4261 acaacgatcc tgtttcgcga cttctcggcc ctgtggattg gccgcacgct ggactgggtt
     4321 tctaaaacct tcggttggta ctatctactg gcggcaacgc tctatattgt ctttgtggtc
     4381 tgtatcgctt gttcgcgttt tggttcggtg aagctcgggc cagaacaatc caaaccggaa
     4441 ttcagcctgc tgagttgggc ggcgatgctg tttgctgccg ggatcggtat cgacctgatg
     4501 ttcttctccg tagccgaacc ggtaacgcag tatatgcagc cgccggaagg cgcgggacag
     4561 acgattgagg ccgcgcgtca ggcgatggtc tggacgctgt ttcactacgg cttaaccggc
     4621 tggtcgatgt atgcgctgat gggcatggcg ctcggatact ttagctatcg ttataatttg
     4681 ccgctcacca tccgctcggc gctgtacccg atcttcggta aacggattaa cgggccgata
     4741 gggcactcag tggatattgc agcggtgatc ggcaccatct tcggtattgc cactacgctc
     4801 ggtatcggtg tggtgcagct taactatggc ttgagcgtac tgtttgatat tcccgattcg
     4861 atggcggcaa aagcggcact gatcgccttg tcggtgataa tcgccacgat ctctgtcacc
     4921 tccggtgtcg ataaggggat tcgtgtgtta tcggaactta acgttgcgct ggcgctggga
     4981 ttgatcctgt tcgtattgtt tatgggcgac acctcgttcc tgcttaatgc gctggtgctg
     5041 aatgttggcg actatgtgaa tcgctttatg ggcatgacgc tcaacagttt tgccttcgac
     5101 cgtccagttg agtggatgaa taactggacg ctcttcttct gggcatggtg ggtggcatgg
     5161 tcgccgtttg tcggcttgtt cctggcgcgt atctcgcgtg ggcgtaccat tcgccagttc
     5221 gtgctgggca cgttgattat tccgtttacc ttcacgctgt tatggctctc ggtgttcggc
     5281 aatagcgcgc tgtatgaaat catccacggc ggcgcggcat ttgccgagga agcgatggtc
     5341 catccggagc gcggcttcta cagcctgctg gcgcagtatc cggcgtttac ctttagcgcc
     5401 tccgtcgcca ccattactgg cctgctgttt tatgtgacct cggcggactc cggtgcgctg
     5461 gtgctgggga atttcacctc gcagcttaaa gatatcaaca gcgacgcccc cggctggctg
     5521 cgcgtcttct ggtcggtggc gattggcctg ctgacgctcg gcatgctgat gaccaacggg
     5581 atatccgcgc tgcaaaacac cacggtgatt atggggctgc cgttcagctt tgtgatcttc
     5641 ttcgtgatgg cggggttgta taaatctctg aaggtagaag attaccgccg tgaaagtgcc
     5701 aaccgcgata ccgcaccgcg accgctgggg cttcaggatc gcctgagctg gaaaaaacgt
     5761 ctctcgcgcc tgatgaatta tccgggcacg cgttacacta aacagatgat ggagacggtc
     5821 tgttacccgg caatggaaga agtggcgcag gagctgcggt tgcgcggcgc atacgtggag
     5881 ctaaaaagcc tgccgccgga agagggacaa cagttggggc atctggattt gttggtgcat
     5941 atgggcgaag aacaaaactt tgtctatcag atttggccgc agcaatattc ggtgccgggc
     6001 tttacctacc gcgcacgtag cggtaaatcg acctactacc ggctggaaac cttcctgtta
     6061 gaaggcagcc agggcaacga cctgatggac tacagcaaag agcaggtgat caccgatatt
     6121 cttgaccagt acgagcggca ccttaacttt attcatctcc atcgtgaagc gccgggccat
     6181 agcgtgatgt tcccggacgc gtgattgttt ttgcataaaa ccgcgccatg tctgcatatg
     6241 gcgcgggaat cattgac
//
LOCUS       DAZKHF020000251         6242 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2201S31241
            SAMN43267800-rid25106413.denovo.253, whole genome shotgun sequence.
ACCESSION   DAZKHF020000251 DAZKHF020000000
VERSION     DAZKHF020000251.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267800
            Sequence Read Archive: SRR30307263
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6242)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6242)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 6242)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350581.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350581.2
            Genome Coverage       :: 151x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:21:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,652
            CDSs (total)                      :: 5,557
            Genes (coding)                    :: 5,320
            CDSs (with protein)               :: 5,320
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 237
            CDSs (without protein)            :: 237
            Pseudo Genes (ambiguous residues) :: 0 of 237
            Pseudo Genes (frameshifted)       :: 102 of 237
            Pseudo Genes (incomplete)         :: 130 of 237
            Pseudo Genes (internal stop)      :: 52 of 237
            Pseudo Genes (multiple problems)  :: 41 of 237
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6242
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267800-rid25106413.denovo.253"
                     /serotype="O 26:H11"
                     /isolate="2201S31241"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-01"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            complement(270..746)
                     /locus_tag="ACHOSC_RS20045"
                     /old_locus_tag="pgaptmp_004009"
     CDS             complement(270..746)
                     /locus_tag="ACHOSC_RS20045"
                     /old_locus_tag="pgaptmp_004009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="kinase inhibitor"
                     /protein_id="HGU4549842.1"
                     /translation="MKLISNDLRDGDKLPHRHVFNGMGYDGDNISPHLAWDDVPAGTK
                     SFVVTCYDPDAPTGSGWWHWVVVNLPADTRVLPQGFGSGLVAMPDGVLQTRTDFGKTG
                     YDGAAPPKGETHRYIFTVHALDIERIDVDEGASGAMVGFNVHFHSLASASITAMFS"
     gene            complement(805..2094)
                     /gene="bioA"
                     /locus_tag="ACHOSC_RS20050"
                     /old_locus_tag="pgaptmp_004010"
     CDS             complement(805..2094)
                     /gene="bioA"
                     /locus_tag="ACHOSC_RS20050"
                     /old_locus_tag="pgaptmp_004010"
                     /EC_number="2.6.1.62"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004015 -
                     adenosylmethionine-8-amino-7-oxononanoate transaminase
                     activity [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009102 - biotin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylmethionine--8-amino-7-oxononanoate
                     transaminase"
                     /protein_id="HGU4549843.1"
                     /translation="MTTDDLAFDQRHIWHPYTSMTSPLPVYPVASAEGCELILSDGRR
                     LVDGMSSWWAAIHGYNHPQLNAAMKSQIDAMSHVMFGGITHAPAIELCRKLVAMTPQP
                     LECVFLADSGSVAVEVAMKMALQYWQAKGEARQRFLTFRNGYHGDTFGAMSVCDPDNS
                     MHSLWKGYLPENLFAPAPQSRMDGEWDERDMVGFARLMAAHRHEIAAVIIEPIVQGAG
                     GMRMYHPEWLKRIRKMCDREGILLIADEIATGFGRTGKLFACEHAEIAPDILCLGKAL
                     TGGTMTLSATLTTREVAETISNGEAGCFMHGPTFMGNPLACAAANASLAILESGDWQQ
                     QVAAIEVQLREQLAPARDAEMVADVRVLGAIGVVETTHPVNMAALQKFFVEQGVWIRP
                     FGKLIYLMPPYIILPQQLQRLTAAVNRAVQDETFFCQ"
     gene            2181..3221
                     /gene="bioB"
                     /locus_tag="ACHOSC_RS20055"
                     /old_locus_tag="pgaptmp_004011"
     CDS             2181..3221
                     /gene="bioB"
                     /locus_tag="ACHOSC_RS20055"
                     /old_locus_tag="pgaptmp_004011"
                     /EC_number="2.8.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004076 - biotin synthase activity
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0009102 - biotin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biotin synthase BioB"
                     /protein_id="HGU4549844.1"
                     /translation="MAHRPRWTLSQVTELFEKPLLDLLFEAQQVHRQHFDPRQVQVST
                     LLSIKTGACPEDCKYCPQSSRYKTGLEAERLMEVEQVLESARKAKAAGSTRFCMGAAW
                     KNPHERDMPYLEQMVQGVKAMGLEACMTLGTLSESQAQRLANAGLDYYNHNLDTSPEF
                     YGNIITTRTYQERLDTLEKVRDAGIKVCSGGIVGLGETVKDRAGLLLQLANLPTPPES
                     VPINMLVKVKGTPLADNDDVDAFDFIRTIAVARIMMPTSYVRLSAGREQMNEQTQAMC
                     FMAGANSIFYGCKLLTTPNPEEDKDLQLFRKLGLNPQQTAVLAGDNEQQQRLEQALMT
                     PDTDEYYNAAAL"
     gene            3218..4372
                     /gene="bioF"
                     /locus_tag="ACHOSC_RS20060"
                     /old_locus_tag="pgaptmp_004012"
     CDS             3218..4372
                     /gene="bioF"
                     /locus_tag="ACHOSC_RS20060"
                     /old_locus_tag="pgaptmp_004012"
                     /EC_number="2.3.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008710 - 8-amino-7-oxononanoate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009102 - biotin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="8-amino-7-oxononanoate synthase"
                     /protein_id="HGU4549845.1"
                     /translation="MSWQEKINAALDARRAADALRRRYPVAQGAGRWLVADDRQYLNF
                     SSNDYLGLSHHPQIIRAWQQGAEQFGVGSGGSGHVSGYSVAHQALEEELAEWLGYSRA
                     LLFISGFAANQAVIAAMMAKEDRIVADRLSHASLLEAASLSPSQLRRFVHNDVTHLAR
                     LLASPCPGQQLVVTEGVFSMDGDIAPLAEIQQVTQQHNGWLMVDDAHGTGVIGEQGRG
                     SCWLQKVKPELLVVTFGKGFGVSGAAVLCSSTVADYLLQFARHLIYSTSMPPAQAQAL
                     RASLAVIRRDEGDARREKLVSLIARFRAGVQDLPFTLADSCSAIQPLIVGDNSRALQL
                     AEKLRQQGCWVTAIRPPTVPAGTARLRLTLTAAHEMQDIDRLLEVLHGNG"
     gene            4359..5114
                     /gene="bioC"
                     /locus_tag="ACHOSC_RS20065"
                     /old_locus_tag="pgaptmp_004013"
     CDS             4359..5114
                     /gene="bioC"
                     /locus_tag="ACHOSC_RS20065"
                     /old_locus_tag="pgaptmp_004013"
                     /EC_number="2.1.1.197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010340 - carboxyl-O-methyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009102 - biotin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malonyl-ACP O-methyltransferase BioC"
                     /protein_id="HGU4549846.1"
                     /translation="MATVNKQAIAAAFGRAAALYEQHADLQRQSADVLLAMLPQRKYT
                     HVLDAGCGPGWMSRHWRERHAQVTALDLSPPMLVQARQKDAADHYLAGDIESLPLATA
                     TFDLAWSNLAVQWCGNLSTALRELYRVVRPGGVVAFTTLVQGSLPELHQAWQAVDERP
                     HANRFLPPDEIEQSLNGVHYQHHIQPITLWFDDALSAMRSLKGIGATHLHEGRDPRIL
                     TRSQLQRLQLAWPQQQGRYPLTYHLFLGVIARE"
     gene            5107..5784
                     /gene="bioD"
                     /locus_tag="ACHOSC_RS20070"
                     /old_locus_tag="pgaptmp_004014"
     CDS             5107..5784
                     /gene="bioD"
                     /locus_tag="ACHOSC_RS20070"
                     /old_locus_tag="pgaptmp_004014"
                     /EC_number="6.3.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706656.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004141 - dethiobiotin synthase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0009102 - biotin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dethiobiotin synthase"
                     /protein_id="HGU4549847.1"
                     /translation="MSKRYFVTGTDTEVGKTVASCALLQAAKAVGYRTAGYKPVASGS
                     EKTPEGLRNSDALALQHNSSLQLDYATVNPYTFAEPTSPHIISAQEGRPIESLVMSAG
                     LRALEQQADWVLVEGAGGWFTPLSDTFTFADWVTQEQLPVILVVGVKLGCINHAMLTA
                     QAIQHAGLTLAGWVANDVTPPGKRHAEYMTTLTRMIPAPLLGEIPWLAENPENAATGK
                     YINLALL"
BASE COUNT         1488 a         1624 c         1728 g         1402 t
ORIGIN      
        1 aaaatcacct tgcgctaatg ctctgtctca ggtcactaat actatctaag tagttgattc
       61 atagttacta gatatgttgt gttttgtagt attatgcagt ctatttttta gactaaatat
      121 attgtaacac attgatatta atggtcttta gcgtttcgcg ttcagctttt ttatactaac
      181 ttgagcgaaa cgggaaggta aaaagacaaa aagttgtttt taataccttt aagtgatacc
      241 agatggcatt gcgccatctg gcagagtgat taactaaaca tcgcagtaat cgaggcgctt
      301 gccagagagt ggaaatgaac gttaaacccg accatcgcgc cgctggcacc ttcatcgaca
      361 tcaatacgtt ctatatccag cgcgtgaacg gtaaaaatgt agcgatgagt ttcgcctttc
      421 ggcggtgctg cgccatcgta cccggtttta ccaaagtcgg tacgcgtctg caaaacgccg
      481 tctggcattg ctaccagacc agagccaaac ccttgcggta atacgcgggt atcagcgggt
      541 aagttaacaa ctacccagtg ccaccagccg gagccggttg gcgcatccgg gtcgtagcag
      601 gtgacaacaa aacttttcgt tcccgcagga acatcatccc acgccagatg cggtgaaata
      661 ttatcgccat cgtaacccat gccgttaaag acatgacgat gcggcaattt atcgccatcg
      721 cgcagatcgt tactgatgag tttcatgaac cctcctttct tgtttgcaga aagtgtagcc
      781 agaaaccctc acgcggactt ctcgttattg gcaaaaaaat gtttcatcct gtaccgcgcg
      841 gttaaccgct gcggtcagac gctgcaactg ttgcgggaga ataatatagg gcggcatcag
      901 gtaaatcagt ttgccaaaag gccggatcca gacaccctgt tcgacaaaga atttttgcag
      961 cgccgccata ttcaccggat gagtggtttc gactacgccg attgccccca gcacgcgcac
     1021 atcggcaacc atttcggcat cacgggcggg ggcaagttgc tcgcgcagct gtacttcaat
     1081 agccgccacc tgttgctgcc agtcgccaga ttcgagaatc gccaggctgg cgtttgctgc
     1141 cgcgcaggcc agcggattgc ccataaaagt tggcccatgc ataaagcaac cggcttcgcc
     1201 gttactgatg gtttccgcaa cctcgcgcgt ggtgagcgtg gcggaaaggg tcattgtgcc
     1261 gccggttaag gctttaccga ggcacaaaat gtccggcgcg atttctgcat gttcacaggc
     1321 aaacagtttg ccggtacgac caaatccggt ggcgatctcg tcggcaatca gcaagatacc
     1381 ttcgcgatcg cacattttgc ggattcgttt taaccattcc ggatggtaca tgcgcatccc
     1441 gcctgcgccc tggacaatcg gttcaatgat caccgccgcg atttcatgac gatgcgccgc
     1501 catcaggcgg gcaaagccaa ccatatcacg ttcatcccat tcaccatcca tgcggctttg
     1561 cggggcggga gcaaacaagt tttctggcag atagcctttc cacaggctgt gcattgagtt
     1621 gtccggatcg cacaccgaca tcgcgccaaa ggtatcgcca tgataaccat tgcggaaggt
     1681 cagaaaacgc tggcgcgctt cgcctttggc ctgccagtac tgcaacgcca ttttcatcgc
     1741 cacttccacc gctacggagc cggagtccgc gagaaaaacg cattccagcg gttgcggcgt
     1801 cattgccacc agtttgcggc atagctcgat ggcgggcgca tgggtgatac cgccaaacat
     1861 cacatgcgac atagcatcaa tttgcgactt catcgccgca ttaagctgcg ggtgattata
     1921 gccgtggatc gccgcccacc aggacgacat accgtcaacc aggcgtctgc cgtcagacaa
     1981 aattagctcg caaccttcgg cgctcgcgac cggataaacc ggcagagggg aggtcatgga
     2041 tgtgtatggg tgccagatat ggcgttggtc aaaggcaaga tcgtccgttg tcataatcga
     2101 cttgtaaacc aaattgaaaa gatttaggtt tacaattcta caccgaatta acaacaaaaa
     2161 acacgttttg gagaagcccc atggctcacc gcccacgctg gacattgtcg caagtcacag
     2221 aattatttga aaaaccgttg ctggatctgc tgtttgaagc gcagcaggtg catcgacagc
     2281 atttcgatcc tcgtcaggtg caggtcagca ctttgctgtc gattaagacc ggagcttgtc
     2341 cggaagattg caaatactgc ccgcaaagct cgcgctacaa aaccgggctg gaagccgagc
     2401 ggttgatgga agttgaacag gtgctggagt cggcgcgcaa agcgaaagct gcgggatcga
     2461 cgcgtttctg tatgggcgcg gcgtggaaga atccccacga acgcgatatg ccgtacctgg
     2521 aacaaatggt gcagggggta aaagcgatgg ggctggaggc gtgtatgacg ctgggcacgt
     2581 tgagtgaatc tcaggcgcag cgccttgcga atgccgggct ggattactac aaccacaacc
     2641 tcgacacctc gccggagttt tacggcaata tcatcaccac ccgcacttat caggaacgcc
     2701 tcgatacgct ggaaaaagtg cgcgatgccg ggatcaaagt ctgttctggc ggcattgtgg
     2761 gcttaggcga aacggtaaaa gatcgcgccg gattattgct gcaactggca aacctgccga
     2821 cgccgccgga aagcgtacca atcaacatgc tggtgaaggt gaaaggcacg ccgctggcgg
     2881 ataacgatga tgtcgatgcc tttgatttta ttcgcaccat tgcggtcgcg cggatcatga
     2941 tgccgacctc ttacgtgcgc ctttctgccg gacgcgagca gatgaacgaa cagactcagg
     3001 cgatgtgctt tatggcaggc gcaaactcga ttttctacgg ttgcaaactg ctgaccacgc
     3061 cgaatccgga agaagataaa gacctgcaac tgttccgcaa actggggcta aatccgcagc
     3121 aaactgccgt gctggcaggg gataacgaac aacagcaacg tcttgaacag gcgctgatga
     3181 ccccggacac cgacgaatat tacaacgcgg cagcattatg agctggcagg agaaaatcaa
     3241 cgcggcgctc gatgcgcggc gtgctgccga tgccctgcgt cgccgttatc cagtggcgca
     3301 aggagccgga cgctggctgg tggcggatga tcgccagtat ctgaactttt ccagtaacga
     3361 ttatttaggt ttaagccatc atccgcaaat tatccgtgcc tggcagcagg gtgcggagca
     3421 atttggcgtc ggtagcggcg gttccggtca cgtcagcggt tatagcgttg cgcatcaggc
     3481 gctggaagaa gaactggccg agtggctggg ctattcgcgg gcactgctat ttatctctgg
     3541 ttttgccgct aatcaggcgg tcattgccgc gatgatggca aaagaggacc gcattgtcgc
     3601 tgaccggctt agccatgcct cattgctgga ggctgccagt ttaagcccgt cgcagcttcg
     3661 ccgttttgtt cataacgatg tcactcatct ggcgcgactg cttgcttccc cctgtccggg
     3721 gcagcaactg gtggtgacag aaggcgtgtt cagcatggac ggcgatattg cgccactggc
     3781 ggaaatccag caggtaacgc aacagcacaa tggctggttg atggtcgatg atgcccacgg
     3841 cacgggcgtt atcggggagc aggggcgcgg cagctgctgg ctgcaaaagg taaaaccaga
     3901 attgctggtg gtgacttttg gcaaaggatt tggcgtcagt ggggctgcgg tgctttgctc
     3961 cagtacggtg gcggattatc tgctgcaatt cgcccgccac cttatctaca gcaccagtat
     4021 gccgcccgct caggcgcagg cattacgtgc gtcactggcg gtcattcgcc gtgatgaggg
     4081 ggatgcacgg cgcgaaaaac tggtgtcact cattgcgcgt tttcgtgccg gagtacagga
     4141 tttgccgttt acgctggctg attcatgcag cgccatccag ccattgatcg tcggtgataa
     4201 cagccgtgcg ttacaactgg cagaaaaact gcgccagcaa ggctgctggg tcacggcgat
     4261 tcgcccgcca accgtacccg ctggtactgc gcgactgcgc ttaacgctaa ccgcagcgca
     4321 tgaaatgcag gatatcgacc gtctgctgga ggtgctgcat ggcaacggtt aataaacaag
     4381 ccattgcagc ggcatttggt cgggcagccg cactctatga gcaacacgca gatctacagc
     4441 gccagagtgc tgacgtctta ctggcaatgc tcccacagcg taaatacacc cacgtactgg
     4501 acgcgggttg tggacctggc tggatgagcc gccactggcg ggaacgtcac gcgcaggtga
     4561 cggccttaga tctctcgccg ccaatgcttg ttcaggcacg ccagaaggat gccgcagacc
     4621 attatctggc gggagatatc gaatccctgc cgttagcgac tgcgacgttc gatcttgcat
     4681 ggagcaatct cgcagtgcag tggtgcggta atttatccac ggcactccgc gagctgtatc
     4741 gggtggtgcg ccccggaggc gtggtggcgt ttaccacgct ggtgcaggga tcgttacccg
     4801 aactgcatca ggcgtggcag gcggtggacg agcgtccgca tgctaatcgc tttttaccgc
     4861 cagatgaaat cgaacagtcg ctgaacggcg tgcattatca acatcatatt cagcccatca
     4921 cactatggtt tgatgatgcg ctcagtgcca tgcgttcgct gaaaggcatc ggtgctacgc
     4981 atcttcatga agggcgcgac ccgcgaatat taacgcgttc gcaattgcag cgattgcaac
     5041 tggcctggcc gcaacaacag gggcgatatc ctctgacgta tcatcttttt ttgggagtga
     5101 ttgctcgtga gtaaacgtta ttttgtcacc ggaacggata ccgaagtggg gaaaaccgtc
     5161 gccagttgtg cacttttaca agccgcgaag gcagtaggtt atcggacggc aggttataaa
     5221 ccggtcgcct ctggcagcga aaagaccccg gaaggcttac gtaatagtga cgcgctggcg
     5281 ttacagcaca acagcagcct gcagctggat tacgcaacgg taaatcctta caccttcgca
     5341 gaacccacct cgccgcacat catcagcgcg caagagggca ggccgataga atcattggta
     5401 atgagcgccg gattacgcgc gcttgaacaa caggctgact gggtgttagt ggaaggtgct
     5461 ggcggctggt ttacgccgct ttctgacact ttcacttttg cagattgggt aacacaggaa
     5521 caactgccgg tgatactggt agttggtgtg aaactcggct gtattaatca cgcgatgttg
     5581 actgcgcagg caatacaaca cgccggactg actctggcgg gttgggtggc gaacgatgtt
     5641 acgcctccgg gaaaacgtca cgctgaatat atgaccacgc tcacccgcat gattcccgcg
     5701 ccgctgctgg gagagatccc ctggcttgca gaaaatccag aaaatgcggc aaccggaaag
     5761 tacataaacc ttgccttgtt gtagctatcc tgtatttggt taaattgcga gcgagatcgc
     5821 gtcttcgatt gactgcaatt taaccaatta aattctaaaa taatcacgaa aaaaatttta
     5881 cttccgcctc atgcggcgaa tgtgggaatt gcccaggcgg cgggggatag gggctggaga
     5941 cagttatcca ctattcctgt ggataaccat gtgtattaga gttagaaaac acgaggcaag
     6001 cgagagaata cgcggcttgc acgcgaattg gcgttaaaga cggctcaaag aaatatcttt
     6061 tattttttaa ctggttagat aaatgcaatg gcagtcactg aacaggcatc tcttgccata
     6121 aaactgtcat cactcatctt gacaaatgtt aaaaaagccg ttgctttggg gataacccgg
     6181 taaggccgga gttttatctc gccacagagt aaattttgct catgattgac agcggagttt
     6241 ac
//
LOCUS       DAZKHF020000252         6209 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2201S31241
            SAMN43267800-rid25106413.denovo.254, whole genome shotgun sequence.
ACCESSION   DAZKHF020000252 DAZKHF020000000
VERSION     DAZKHF020000252.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267800
            Sequence Read Archive: SRR30307263
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6209)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6209)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 6209)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350581.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350581.2
            Genome Coverage       :: 151x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:21:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,652
            CDSs (total)                      :: 5,557
            Genes (coding)                    :: 5,320
            CDSs (with protein)               :: 5,320
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 237
            CDSs (without protein)            :: 237
            Pseudo Genes (ambiguous residues) :: 0 of 237
            Pseudo Genes (frameshifted)       :: 102 of 237
            Pseudo Genes (incomplete)         :: 130 of 237
            Pseudo Genes (internal stop)      :: 52 of 237
            Pseudo Genes (multiple problems)  :: 41 of 237
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6209
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267800-rid25106413.denovo.254"
                     /serotype="O 26:H11"
                     /isolate="2201S31241"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-01"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            <1..543
                     /locus_tag="ACHOSC_RS20075"
                     /old_locus_tag="pgaptmp_004015"
     CDS             <1..543
                     /locus_tag="ACHOSC_RS20075"
                     /old_locus_tag="pgaptmp_004015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_461669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4549848.1"
                     /translation="AIIELNNLKTSLDRILLSRQFSEIESVIFTFAEPLPIVVSSILS
                     PERDFDGTKIQDLSDLTVSAEQVCFNAFSCGGKGYVVFSWLRTSTIIRRFIRTLIKIP
                     ANRMFNTLLYFFFTKAENTYSSPEWWDRLSDKQRENIGNMIMSGVEFFGRPTSQVDHS
                     VDYNAAALEEITCSNNEICS"
     gene            830..1090
                     /locus_tag="ACHOSC_RS20080"
                     /old_locus_tag="pgaptmp_004016"
     CDS             830..1090
                     /locus_tag="ACHOSC_RS20080"
                     /old_locus_tag="pgaptmp_004016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001512982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ogr/Delta-like zinc finger family protein"
                     /protein_id="HGU4549849.1"
                     /translation="MFRCPLCGASARIRTSRPENDSNTVRQKYYQCNNLECGVCFSTL
                     EAFHKFTSKHASGVHSSEGIPWHELPASHRGNNQMSLPLPQN"
     gene            1281..1421
                     /locus_tag="ACHOSC_RS20085"
                     /old_locus_tag="pgaptmp_004017"
     CDS             1281..1421
                     /locus_tag="ACHOSC_RS20085"
                     /old_locus_tag="pgaptmp_004017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000078918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hok/Gef family protein"
                     /protein_id="HGU4549850.1"
                     /translation="MSQKSLTAITFCVTVILIIWMLRGSLCEIRMSFWGAEFAAFLQC
                     KQ"
     gene            complement(1610..1891)
                     /locus_tag="ACHOSC_RS20090"
                     /old_locus_tag="pgaptmp_004018"
     CDS             complement(1610..1891)
                     /locus_tag="ACHOSC_RS20090"
                     /old_locus_tag="pgaptmp_004018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_305382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4549851.1"
                     /translation="MRCGLLMAICLCLVSFSGYAATGKSDAQIKKEIIKESIESYPGN
                     CACPYNHARNGSRCGGRSAYSRNGGYDVVCYEGDVTDEMIRQWKQENAE"
     gene            complement(2430..2516)
                     /locus_tag="ACHOSC_RS20095"
                     /old_locus_tag="pgaptmp_004019"
     tRNA            complement(2430..2516)
                     /locus_tag="ACHOSC_RS20095"
                     /old_locus_tag="pgaptmp_004019"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2480..2482),aa:Leu,seq:taa)
     gene            complement(2529..2602)
                     /locus_tag="ACHOSC_RS20100"
                     /old_locus_tag="pgaptmp_004020"
     tRNA            complement(2529..2602)
                     /locus_tag="ACHOSC_RS20100"
                     /old_locus_tag="pgaptmp_004020"
                     /product="tRNA-Cys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2568..2570),aa:Cys,seq:gca)
     gene            complement(2657..2732)
                     /locus_tag="ACHOSC_RS20105"
                     /old_locus_tag="pgaptmp_004021"
     tRNA            complement(2657..2732)
                     /locus_tag="ACHOSC_RS20105"
                     /old_locus_tag="pgaptmp_004021"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2697..2699),aa:Gly,seq:gcc)
     gene            complement(2884..3432)
                     /gene="pgsA"
                     /locus_tag="ACHOSC_RS20110"
                     /old_locus_tag="pgaptmp_004022"
     CDS             complement(2884..3432)
                     /gene="pgsA"
                     /locus_tag="ACHOSC_RS20110"
                     /old_locus_tag="pgaptmp_004022"
                     /EC_number="2.7.8.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol--glycerol-3-phosphate
                     3-phosphatidyltransferase"
                     /protein_id="HGU4549852.1"
                     /translation="MQFNIPTLLTLFRVILIPFFVLVFYLPVTWSPFAAALIFCVAAV
                     TDWFDGFLARRWNQSTRFGAFLDPVADKVLVAIAMVLVTEHYHSWWVTLPAATMIARE
                     IIISALREWMAELGKRSSVAVSWIGKVKTTAQMVALAWLLWRPNIWVEYAGIALFFVA
                     AVLTLWSMLQYLSAARADLLDQ"
     gene            complement(3489..5321)
                     /gene="uvrC"
                     /locus_tag="ACHOSC_RS20115"
                     /old_locus_tag="pgaptmp_004023"
     CDS             complement(3489..5321)
                     /gene="uvrC"
                     /locus_tag="ACHOSC_RS20115"
                     /old_locus_tag="pgaptmp_004023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310678.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009380 - excinuclease repair complex
                     [Evidence IEA];
                     GO_function: GO:0009381 - excinuclease ABC activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrC"
                     /protein_id="HGU4549853.1"
                     /translation="MSDQFDAKAFLKTVTSQPGVYRMYDAGGTVIYVGKAKDLKKRLS
                     SYFRSNLASRKTEALVAQIQQIDVTVTHTETEALLLEHNYIKLYQPRYNVLLRDDKSY
                     PFIFLSGDTHPRLAMHRGAKHAKGEYFGPFPNGYAVRETLALLQKIFPIRQCENSVYR
                     NRSRPCLQYQIGRCLGPCVEGLVSEEEYAQQVEYVRLFLSGKDDQVLTQLISRMETAS
                     QNLEFEEAARIRDQIQAVRRVTEKQFVSNTGDDLDVIGVAFDAGMACVHVLFIRQGKV
                     LGSRSYFPKVPGGTELSEVVETFVGQFYLQGSQMRTLPGEILLDFNLSDKTLLADSLS
                     ELAGRKINVQTKPRGDRARYLKLARTNAATALTSKLSQQSTVHQRLTALASVLKLPEV
                     KRMECFDISHTMGEQTVASCVVFDANGPLRAEYRRYNITGITPGDDYAAMNQVLRRRY
                     GKAIDDSKIPDVILIDGGKGQLAQAKNVFAELDVSWDKNHPLLLGVAKGVDRKAGLET
                     LFFEPEGEGFSLPPDSPALHVIQHIRDESHDHAIGGHRKKRAKVKNTSSLETIEGVGP
                     KRRQMLLKYMGGLQGLRNASVEEIAKVPGISQGLAEKIFWSLKH"
     gene            complement(5318..5974)
                     /gene="uvrY"
                     /locus_tag="ACHOSC_RS20120"
                     /old_locus_tag="pgaptmp_004024"
     CDS             complement(5318..5974)
                     /gene="uvrY"
                     /locus_tag="ACHOSC_RS20120"
                     /old_locus_tag="pgaptmp_004024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UvrY/SirA/GacA family response regulator
                     transcription factor"
                     /protein_id="HGU4549854.1"
                     /translation="MINVLLVDDHELVRAGIRRILEDIKGIKVVGEASCGEDAVKWCR
                     ANAVDVVLMDMSMPGIGGLEATRKIARSTADVKIIMLTVHTENPLPAKVMQAGAAGYL
                     SKGAAPQEVVSAIRSVYSGQRYIASDIAQQMALSQIEPEKTESPFASLSERELQIMLM
                     ITKGQKVNEISEQLNLSPKTVNSYRYRMFSKLNIHGDVELTHLAIRHGLCNAETLSSQ
                     "
     gene            6116..>6209
                     /locus_tag="ACHOSC_RS20125"
                     /old_locus_tag="pgaptmp_004025"
     CDS             6116..>6209
                     /locus_tag="ACHOSC_RS20125"
                     /old_locus_tag="pgaptmp_004025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001302039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HGU4549855.1"
                     /translation="MVTPVTNGLPDRFTVSPATKCDKNYLYSAKM"
BASE COUNT         1655 a         1567 c         1413 g         1574 t
ORIGIN      
        1 gcaataattg aattaaataa tcttaaaact agcctcgacc gcatcctact tagcaggcaa
       61 ttttcagaaa ttgaaagtgt tatcttcact tttgcagagc ctttaccaat tgtagtttcg
      121 tcaattcttt caccggagag ggatttcgat gggaccaaaa ttcaggattt gagcgatctt
      181 acagtgtcgg cggaacaagt atgtttcaat gctttttcct gcggaggtaa gggatatgta
      241 gtgttcagtt ggctgagaac ttcaacaatt atcagacgtt ttatacggac actaattaag
      301 atcccagcta acaggatgtt caataccctt ctgtattttt tcttcacaaa ggccgaaaat
      361 acatattcat cccctgagtg gtgggacagg ctcagtgata aacagcgaga gaatatcggc
      421 aacatgatta tgtcaggggt tgaatttttt ggcagaccta catcacaggt tgatcattcc
      481 gttgattata acgccgctgc acttgaagag ataacatgca gcaacaacga aatttgcagc
      541 tgaatgttta catgctgtta gaaaaagcgg gctgaacaat catttcggga ttgtaacaga
      601 taagatggca ctgcccgctt gcttcccgat atgctcagcc aataaactta tgtaagcaac
      661 cgatttccat ttatgcggag gttatcaaag ttgttggagt tgcgaacttc cgctcttcgc
      721 acaaagcgta ccgctaggtt tgattgtatt ctggctgcgc aaactgtcag ttggagtctg
      781 agctattaca acttagaata gcggcagcac cacgttaagg gaggtcgcta tgttccgttg
      841 tccgctttgt ggcgcatctg cccgaatccg caccagtcgt ccggaaaacg attcaaacac
      901 tgtgcggcaa aagtattacc agtgtaacaa cctggaatgc ggcgtatgct tctcaacact
      961 ggaagctttc cataaattca catcaaaaca cgcctccggc gttcactctt cggaaggtat
     1021 cccgtggcat gagctgccag cttcacacag gggaaacaat cagatgagtt tgcctttacc
     1081 tcagaattaa caggcagaat tgccggagta acaaaaaaac gatagattac ccgcgggtgc
     1141 ctttcggctg atggtcggag gaatacccga aggccggatg tggaaaggcc ccggaaaaca
     1201 tttctgttta accgaggccc taacatatct accttaagca agtgataggt tagcgcctct
     1261 ccaacaaagg agcaagcgct atgtcgcaaa aatcgcttac agccatcacg ttctgcgtga
     1321 cggtaatcct catcatctgg atgctacgcg gttcgctgtg tgaaatacgg atgagcttct
     1381 ggggagcgga gtttgcggcg ttcttacagt gtaagcagta aggaaaccgc gacgggggag
     1441 caatcccccg tcaatcggtt gctaaggttg gctgaagtgg caccctatct cacagacatg
     1501 aacaacaaac ccgcagcgta agaactgcgg gttttctttt tggaacaatc actagctagt
     1561 gtaccgttaa cggcacataa tgctcgcaca acaaacctgt taaaagagat tactcagcat
     1621 tctcctgctt ccattgccgg atcatttcat cggtaacatc accctcataa cataccacgt
     1681 cataccctcc attacggcta tatgcactgc gtccaccaca tctactacca ttccttgcat
     1741 gattataagg acacgcacaa ttgcctggat aagattcaat ggattcttta attatttctt
     1801 ttttgatctg agcatcagac tttcctgttg ctgcatatcc actaaaagat actaaacaaa
     1861 ggcagattgc cattaataaa ccacatcgca taccgataca ctctattact aaccaatcat
     1921 agcccacatc ataaaatcga gatgaggagt acaatacctt taacaagaag gtgtcataac
     1981 gataatatgt cgtgcaattc atcaaaaaaa agatctatat caaagagata gaactcttat
     2041 cagtgcttca gacatcggca ttcgaggcag cagaaaaaca gtaaatctga cgaatttgcc
     2101 ttttcccaag atgttgtcag attaaaaaga tagattagcg caggtacctg ttggtggatt
     2161 gcaggaggaa aaatgaagtc gagtgaaaaa atcccagaaa aatttttgtt taacagaggt
     2221 cctggttcac tatgcgaaat acggaggagc tgcagaagaa ttctgcttca tccttagagt
     2281 ttaaacagca cgtgcactac cattgtttct gcatatcgcc acaccatcgc cactctaccg
     2341 ccattgcaca aaatacaaat acgaaaaaac cacccgaagg tggtttcacg acactgctta
     2401 ttgctttgat tttattctta tctttcccat ggtacccgga gcgggacttg aacccgcaca
     2461 gcgcgaacgc cgagggattt taaatccctt gtgtctaccg attccaccat ccgggctcgg
     2521 gaagaaagtg gaggcgcgtt ccggagtcga accggactag acggatttgc aatccgctac
     2581 ataaccgctt tgttaacgcg ccaaattctt caggcctttc agccagacat ccgcttgacg
     2641 ccgatgtctt ttaaactgga gcgggaaacg agactcgaac tcgcgacccc gaccttggca
     2701 aggtcgtgct ctaccaactg agctattccc gcattcatca agcaatcagt taatcacttg
     2761 attttattat cgtctggcaa tcagtgccgc cgttcgatgc gttgcattct acttacctgg
     2821 cgcgatgagt caacgatatt tttcaccact tttgatcgtt tgctgaaaat tacgccgaaa
     2881 cgatcactga tcaagcaaat ctgcacgcgc agcgctcaaa tattgcaaca ttgaccacag
     2941 agtcagtacc gcagccacaa agaaaagtgc aataccggcg tactcaaccc aaatgttcgg
     3001 acgccacagc agccaggcca acgccaccat ctgggcagtg gttttcactt tcccaatcca
     3061 ggagacagcc acgctactgc gtttacccaa ctccgccatc cattcgcgta gcgcagaaat
     3121 aataatttca cgggcgatca tcgttgccgc cggtagggtc acccaccagc tgtgataatg
     3181 ctcggttacc agcaccatgg cgatagccac gagaacttta tctgctacag ggtcaaggaa
     3241 agcaccaaac cgggtactct ggttccagcg gcgtgccaga aaaccatcga accagtcagt
     3301 caccgccgcg acgcagaaaa tgagcgcggc ggcaaacggc gaccaggtga caggcagata
     3361 aaagaccaat acaaagaatg ggataaggat gacacggaac agtgtaagca acgtagggat
     3421 attaaattgc ataatgacgg gtaactatct gttgtcagta agattacccc tatgttgcta
     3481 cagagacatc aatgtttcaa cgaccagaag atcttttctg ccagaccttg cgaaataccc
     3541 ggcacttttg caatttcctc gacgctggcg ttacgtaaac cttgcaaacc gcccatatat
     3601 ttcaacaaca tttgccgacg ttttggcccg acgccttcaa tggtttccag ggagctggta
     3661 tttttgacct tcgcccgttt tttacggtgc ccgccaatcg cgtgatcgtg tgattcatcg
     3721 cgaatatgct ggataacatg cagcgcggga gaatctggcg gcaaactgaa tccctcacct
     3781 tccggctcaa agaacagtgt ttccagccca gccttacgat ctactccttt ggcaacgcca
     3841 agtagcagtg gatgattttt atcccatgag acatccagtt cggcgaagac atttttcgcc
     3901 tgcgcaagct ggcctttgcc gccgtcgata aggatcacat ccgggatctt actgtcgtca
     3961 atggctttac cataacgccg acgcagcacc tgattcatcg ccgcataatc atcgcccggc
     4021 gtgatgcctg taatgttata gcgccgatac tccgcacgca gcgggccgtt agcatcaaac
     4081 accacacagg aagcgacggt ttgttcgccc atggtatggc tgatgtcaaa gcactccatc
     4141 cgcttcactt ccggcaattt caacacgctg gcaagcgcgg tcagtcgctg gtgaacggta
     4201 gattgctgcg aaagtttgct ggttaaggcc gtcgccgcat tggtgcgcgc gagtttcaga
     4261 taacgcgccc tatcgccgcg aggtttggtt tgaacattaa tcttgcgtcc cgccagttct
     4321 gaaagggaat cggcgagcag cgttttatcg ctaagattaa aatcgagcag gatctcaccc
     4381 ggtaaggtgc gcatctggct gccttgtaaa tagaactggc ctacgaaggt ttctaccacc
     4441 tcgctcagtt ccgtaccgcc aggcactttc gggaaatagc tgcggctgcc gagcactttg
     4501 ccctgacgaa tgaacaatac gtggacacaa gccatgcccg catcgaacgc cacaccaata
     4561 acgtcgaggt cgtcgccggt attggaaacg aattgttttt cggtgacgcg tcgcaccgcc
     4621 tgaatttggt cgcgaatacg tgcagcttct tcaaactcca gattctggct ggcagtttcc
     4681 atacgactaa tgagttgcgt aagcacctga tcatctttgc cagacaaaaa caggcgcaca
     4741 tactcgacct gctgagcgta ttcttcttca ctcaccagtc cttcaacgca cggtcccaga
     4801 cagcgcccta tctggtattg cagacacgga cgcgagcgat tgcgataaac actattttcg
     4861 cactggcgaa tggggaaaat cttttgcagt agcgccagtg tttcacgtac ggcatagcca
     4921 ttcgggaacg ggccgaaata ttcacctttg gcatgcttcg caccacgatg catcgccaga
     4981 cgcgggtggg tatcgccact caggaagata aaaggataag atttatcatc gcgtagcaaa
     5041 acgttgtaac gcggctgata gagtttgatg tagttgtgtt ccagcaacag cgcttcggtt
     5101 tctgtatgag taaccgttac atcaatttgc tggatctggg cgaccagcgc ttcggttttg
     5161 cgcgaagcga ggttgctacg gaaatagctg gaaagccgtt ttttcaggtc tttcgctttg
     5221 ccgacataga taaccgtacc accagcatcg tacatgcgat aaacgcctgg ctggctggtt
     5281 acggttttta aaaacgcttt tgcgtcaaac tgatcactca ctgacttgat aatgtctccg
     5341 cattacacag accatggcga attgccaggt gagtcagctc aacatcgcca tgaatgttta
     5401 gtttactgaa catacgatag cggtagctgt tcaccgtttt cggactgaga ttgagctgtt
     5461 ctgagatctc attgaccttc tggcccttgg tgatcatcag cataatctgc aattcacgtt
     5521 cagacaaact ggcaaatggg ctttctgttt tttctggttc gatctggctt aacgccattt
     5581 gttgagcgat gtcagaagca atgtaacgct gccctgaata gacagaacga atcgcactca
     5641 cgacttcctg cggagccgcg cctttgctga ggtagcccgc agcaccggcc tgcatgactt
     5701 tcgctggtaa agggttttct gtatggacgg taagcatgat gattttgaca tcagctgtgg
     5761 aacgcgcgat tttacgcgtc gcctcaagac cgccaatgcc cggcatactc atgtccatta
     5821 gcaccacgtc aacggcattt gcccgacacc acttaacggc gtcttcaccg cacgatgcct
     5881 caccgacgac ttttataccc tttatatctt ccagaatgcg tcgtatccct gcgcgcacca
     5941 gttcgtggtc atcaacaagt agaacgttga tcaaaggaat atctccagaa atagggataa
     6001 cgctactgat agttagtcat tcgtatatta gcggttttta ttgcaacttt gaaacgttaa
     6061 aaatggtggg tattcgattt ttctctcgtt tttgtcaatt cgattgtcca cagttatggt
     6121 cacgcccgtc accaacggct taccggatcg tttcacagta agccctgcga cgaaatgtga
     6181 caaaaattac ctttattcag caaaaatga
//
LOCUS       DAZKHF020000253         6177 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2201S31241
            SAMN43267800-rid25106413.denovo.255, whole genome shotgun sequence.
ACCESSION   DAZKHF020000253 DAZKHF020000000
VERSION     DAZKHF020000253.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267800
            Sequence Read Archive: SRR30307263
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6177)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6177)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 6177)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350581.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350581.2
            Genome Coverage       :: 151x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:21:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,652
            CDSs (total)                      :: 5,557
            Genes (coding)                    :: 5,320
            CDSs (with protein)               :: 5,320
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 237
            CDSs (without protein)            :: 237
            Pseudo Genes (ambiguous residues) :: 0 of 237
            Pseudo Genes (frameshifted)       :: 102 of 237
            Pseudo Genes (incomplete)         :: 130 of 237
            Pseudo Genes (internal stop)      :: 52 of 237
            Pseudo Genes (multiple problems)  :: 41 of 237
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6177
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267800-rid25106413.denovo.255"
                     /serotype="O 26:H11"
                     /isolate="2201S31241"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-01"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            complement(97..1017)
                     /gene="speB"
                     /locus_tag="ACHOSC_RS20130"
                     /old_locus_tag="pgaptmp_004026"
     CDS             complement(97..1017)
                     /gene="speB"
                     /locus_tag="ACHOSC_RS20130"
                     /old_locus_tag="pgaptmp_004026"
                     /EC_number="3.5.3.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008783 - agmatinase activity [Evidence
                     IEA];
                     GO_process: GO:0008295 - spermidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="agmatinase"
                     /protein_id="HGU4549856.1"
                     /translation="MSTLGHQYDNSLVSNAFGFLRLPMNFQPYDSDADWVITGVPFDM
                     ATSGRAGGRHGPAAIRQVSTNLAWEHNRFPWNFDMRERLNVVDCGDLVYAFGDAREMS
                     EKLQAHAEKLLAAGKRMLSFGGDHFVTLPLLRAHAKHFGKMALVHFDAHTDTYANGCE
                     FDHGTMFYTAPKEGLIDPNHSVQIGIRTEFDKDNGFTVLDACQVNDRSVDDVIAQVKQ
                     IVGDMPVYLTFDIDCLDPAFAPGTGTPVIGGLTSDRAIKLVRGLKDLNIVGMDVVEVA
                     PAYDQSEITALAAATLALEMLYIQAAKKGE"
     gene            complement(1153..1884)
                     /locus_tag="ACHOSC_RS20135"
                     /old_locus_tag="pgaptmp_004027"
     CDS             complement(1153..1884)
                     /locus_tag="ACHOSC_RS20135"
                     /old_locus_tag="pgaptmp_004027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4549857.1"
                     /translation="MKKWKVRSALVALIVLLAGCSSNAQYNSSASGNVGTAWGGDVHS
                     SVQGVSAERAWRDPAEMIVISYSTNVPSGYDRVYSIRINELEYAIRDGNFNSLPITRV
                     YDSSNNEPRYIVHARVGMNYQLYVRNYSRNTNYEIVATVDGLDVLNGKQGSLNNNGYI
                     VNAGDSLAIKGFRKDKHTEAAFQFANVADSYAANSAQGDVRNTGVIGFAAFELQGQAQ
                     NALPPCSGQAFPADNNGYAPPPCRK"
     gene            complement(2030..4006)
                     /gene="speA"
                     /locus_tag="ACHOSC_RS20140"
                     /old_locus_tag="pgaptmp_004028"
     CDS             complement(2030..4006)
                     /gene="speA"
                     /locus_tag="ACHOSC_RS20140"
                     /old_locus_tag="pgaptmp_004028"
                     /EC_number="4.1.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008792 - arginine decarboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006527 - arginine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biosynthetic arginine decarboxylase"
                     /protein_id="HGU4549858.1"
                     /translation="MSDDMSMGLPSSAGEHGVLRSMQEVAMSSQEASKMLRTYNIAWW
                     GNNYYDVNELGHISVCPDPDVPEARVDLAQLVKTREAQGQRLPALFCFPQILQHRLRS
                     INAAFKRARESYGYNGDYFLVYPIKVNQHRRVIESLIHSGEPLGLEAGSKAELMAVLA
                     HAGMTRSVIVCNGYKDREYIRLALIGEKMGHKVYLVIEKMSEIAIVLDEAERLNVVPR
                     LGVRARLASQGSGKWQSSGGEKSKFGLAATQVLQLVETLREAGRLDSLQLLHFHLGSQ
                     MANIRDIATGVRESARFYVELHKLGVNIQCFDVGGGLGVDYEGTRSQSDCSVNYGLNE
                     YANNIIWAIGDACEENGLPHPTVITESGRAVTAHHTVLVSNIIGVERNEYTVPTAPAE
                     DAPRALQSMWETWQEMHEPGTRRSLREWLHDSQMDLHDIHIGYSSGTFSLQERAWAEQ
                     LYLSMCHEVQKQLDPQNRAHRPIIDELQERMADKMYVNFSLFQSMPDAWGIDQLFPVL
                     PLEGLDQVPERRAVLLDITCDSDGAIDHYIDGDGIATTMPMPEYDPENPPMLGFFMVG
                     AYQEILGNMHNLFGDTEAVDVFVFPDGSVEVELSDEGDTVADMLQYVQLDPKTLLTQF
                     RDQVKKTDLDAELQQQFLEEFEAGLYGYTYLEDE"
     gene            complement(4015..4146)
                     /gene="yqgB"
                     /locus_tag="ACHOSC_RS20145"
                     /old_locus_tag="pgaptmp_004029"
     CDS             complement(4015..4146)
                     /gene="yqgB"
                     /locus_tag="ACHOSC_RS20145"
                     /old_locus_tag="pgaptmp_004029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417414.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid stress response protein YqgB"
                     /protein_id="HGU4549859.1"
                     /translation="MKKKPVAQSERQHTLLENPCAYGLLSQFQAAIVVNCFTLNKII"
     gene            complement(4240..4383)
                     /gene="yqgG"
                     /locus_tag="ACHOSC_RS20150"
                     /old_locus_tag="pgaptmp_004030"
     CDS             complement(4240..4383)
                     /gene="yqgG"
                     /locus_tag="ACHOSC_RS20150"
                     /old_locus_tag="pgaptmp_004030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YqgG"
                     /protein_id="HGU4549860.1"
                     /translation="MNRCLLLNLSHRSGEDSFPALCISALHTCRCYTHLGASQDSRAG
                     YSY"
     gene            complement(4494..4745)
                     /gene="yqgD"
                     /locus_tag="ACHOSC_RS20155"
                     /old_locus_tag="pgaptmp_004031"
     CDS             complement(4494..4745)
                     /gene="yqgD"
                     /locus_tag="ACHOSC_RS20155"
                     /old_locus_tag="pgaptmp_004031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YqgD"
                     /protein_id="HGU4549861.1"
                     /translation="MVADPTTTLQVKNTGSLSVNRYGWINIWMAILGQFFTRFPLFFE
                     SCLILLKTWLEIFPDNAGILRIYLLQFSAIVGYKTRRAA"
     gene            4801..5955
                     /gene="metK"
                     /locus_tag="ACHOSC_RS20160"
                     /old_locus_tag="pgaptmp_004032"
     CDS             4801..5955
                     /gene="metK"
                     /locus_tag="ACHOSC_RS20160"
                     /old_locus_tag="pgaptmp_004032"
                     /EC_number="2.5.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004478 - methionine adenosyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006556 - S-adenosylmethionine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine adenosyltransferase"
                     /protein_id="HGU4549862.1"
                     /translation="MAKHLFTSESVSEGHPDKIADQISDAVLDAILEQDPKARVACET
                     YVKTGMVLVGGEITTSAWVDIEEITRNTVREIGYVHSDMGFDANSCAVLSAIGKQSPD
                     INQGVDRADPLEQGAGDQGLMFGYATNETDVLMPAPITYAHRLVQRQAEVRKNGTLPW
                     LRPDAKSQVTFQYDDGKIVGIDAVVLSTQHSEEIDQKSLQEAVMEEIIKPILPAEWLT
                     SATKFFINPTGRFVIGGPMGDCGLTGRKIIVDTYGGMARHGGGAFSGKDPSKVDRSAA
                     YAARYVAKNIVAAGLADRCEIQVSYAIGVAEPTSIMVETFGTEKVPSEQLTLLVREFF
                     DLRPYGLIQMLDLLHPIYKETAAYGHFGREHFPWEKTDKAQLLRDAAGLK"
BASE COUNT         1589 a         1647 c         1491 g         1450 t
ORIGIN      
        1 agagctaaga aaaagtagcg tagaaatata aaaacatata tcgcactgat gaatgtttcc
       61 cggcatgact atgcacaccg ggaaatattt agtaaattac tcgccctttt tcgccgcctg
      121 aatatacagc atctccagcg ccagcgtcgc cgcagccaga gcagtgattt ccgactgatc
      181 gtatgccgga gccacttcca ctacgtccat cccaacgatg ttgagatctt tcaggccgcg
      241 taccagttta atggcgcgat cggaggtcag accgccaatt accggcgtac cggttcccgg
      301 cgcaaatgca ggatccaggc agtcgatatc aaaggtcagg taaaccggca tatcacccac
      361 aatttgtttc acctgggcga taacgtcatc cacgctgcga tcgttcacct ggcaggcgtc
      421 cagcacggta aagccgttgt ctttatcaaa ttcggtacga ataccaatct gcacggaatg
      481 attcggatcg atcagacctt ctttcggcgc ggtatagaac atggtgccgt ggtcaaattc
      541 acaaccgttc gcgtaggtat cggtgtgggc gtcaaagtgt accagcgcca ttttgccgaa
      601 atgcttcgca tgagcacgca gcagcggcag cgtaacaaag tggtcaccac cgaaggagag
      661 catacgctta ccggcagcca gcagcttctc ggcgtgcgcc tgcagcttct cgctcatctc
      721 acgggcatcg ccgaaagcat ataccagatc accgcagtcc acgacgttca gacgctcacg
      781 catgtcgaaa ttccacggga agcggttgtg ttcccaggcc agattcgtcg aaacctgacg
      841 gatagctgcc ggaccgtggc gtccccccgc acgaccagaa gtggccatat cgaacggcac
      901 accagtaatc acccagtcag catcgctgtc atacggctgg aagttcatcg gcaggcgtaa
      961 aaaaccaaag gcattggaaa ccagtgagtt atcgtattga tgacctaagg tgctcatgga
     1021 tggactccta ttacaaagtc gatataaaaa aacccttccg cgtcgttagg cccgacgagg
     1081 aagggttgga tttgcgaaac acaagtttgt gctaattatc gccttaaatg ggcgaggcat
     1141 caagcgaggt tgctatttcc gacacggtgg cggcgcatag ccattgttat ctgccggaaa
     1201 ggcttgtcca gagcacggcg gcagcgcgtt ttgcgcctgg ccttgtaatt caaaggcggc
     1261 aaagcctatt acaccggtat tacgcacatc gccttgcgcc gaattggctg cgtaggaatc
     1321 agcaacgttg gcgaactgaa aagccgcttc ggtatgttta tctttgcgga atcctttaat
     1381 cgctaaagaa tctccggcat tcacaatata gccgttgtta ttcagcgaac cttgcttgcc
     1441 gttcagcaca tccaggccat caacagtagc aacgatctcg tagttggtgt tacgactgta
     1501 attacggaca tataattgat aattcattcc gactctggca tggacaatat agcgtggttc
     1561 attattggat gaatcataaa cacgcgtaat aggtagagaa ttaaaattac catcgcgaat
     1621 agcatattcc agttcgttaa tacgaatgct ataaacgcgg tcatagccgg aaggaacatt
     1681 agtcgaataa ctaataacga tcatctctgc cggatcgcgc caggctcgct cagcagatac
     1741 gccctgaacc gatgagtgca catccccacc ccaggcagtg ccgacattgc ccgatgcact
     1801 gctattatac tgcgcattac tggaacaacc ggccagcagc acgattaatg ccaccaacgc
     1861 gctacgtact ttccattttt tcataaccgt tccctctgca tttaattaat gcagcgattg
     1921 tagggagatt atttaataag aaaagcagcg agattatttt taagaatata taaaaagaaa
     1981 gcgggccgat ttacgtaaat aaatcgaccc gccagcggaa taatcttaat tattcatctt
     2041 caagataggt ataaccgtac aaacctgcct cgaactcttc aaggaactgt tgttgcagtt
     2101 cagcatcaag atcggttttc ttcacttgat cgcggaactg ggttaacagc gttttcggat
     2161 cgagctgtac atattgcagc atgtccgcca cggtatcgcc ttcgtcagac agttctactt
     2221 ctacgctacc gtcagggaac acgaacacgt caaccgcttc ggtatcaccg aacaggttgt
     2281 gcatattgcc aaggatctcc tgatatgcgc cgaccataaa gaaaccgagc atcggcggat
     2341 tctctggatc gtactccggc attggcattg tcgtggcaat accatcacca tcaatatagt
     2401 ggtcgatagc accgtcagag tcacaggtaa tatccagcag cactgcgcga cgttccggca
     2461 cttgatccag cccttccagc ggcagaaccg ggaacaactg gtcgatcccc catgcatccg
     2521 gcattgactg gaacagcgag aaattgacgt acattttgtc cgccatacgt tcctgcagtt
     2581 cgtcgattat cggacgatgc gcacggtttt gcggatccag ctgcttttgc acttcatggc
     2641 acatgctcag atagagttgt tctgcccatg cacgttcttg caggctaaag gtgccggaag
     2701 agtagccgat atgaatgtcg tgcagatcca tctggctgtc gtgtaaccat tcacgcagag
     2761 aacggcgagt tcccggttcg tgcatttcct gccaggtttc ccacatgctt tgcagcgcgc
     2821 gcggcgcatc ttctgcaggc gcggtcggca ccgtgtattc gttacgttcc acgccgatga
     2881 tattagatac cagcacggtg tgatgcgcag tcaccgcacg acccgattcg gtgattaccg
     2941 tcggatgcgg cagaccgttt tcttcacacg catcaccaat cgcccagatg atgttgttgg
     3001 catattcatt gaggccgtag ttcaccgaac agtcggactg cgaacgggtg ccttcatagt
     3061 ccacgcccag accgccgccg acgtcgaagc actgaatatt gacgcccagc ttgtgcagtt
     3121 ccacatagaa acgcgcggat tcacgaacgc ctgtcgcgat atcgcgaata ttcgccatct
     3181 gcgaaccgag gtggaagtgc agcagttgca ggctgtcgag acgcccggct tcacgcaggg
     3241 tttcaacaag ttgcagtacc tgagtcgcag ccaggccgaa cttcgatttt tccccgccgg
     3301 aggactgcca tttacccgag ccctgcgaag ccagacgtgc acgcacgccc agacgaggaa
     3361 cgacattcag acgttctgct tcatccagca caatggcgat ttctgacatc ttctcaatga
     3421 ccaggtagac cttgtggccc atcttctcgc caattaatgc caggcggata tattcgcggt
     3481 ctttataacc gttgcagacg atgacgctac gggtcatgcc agcatgtgcc agcaccgcca
     3541 tcaactctgc tttcgagcct gcttccagtc ccagcggttc gcccgaatga atcagggact
     3601 caatcacgcg gcggtgctgg ttaactttga tcggataaac aaggaagtaa tcgccgttat
     3661 agccgtagga ttcccgcgca cgtttgaacg cggcgttaat ggaacgcaaa cggtgctgca
     3721 ggatctgtgg gaaacagaac agtgcaggca gacgctgacc ctgtgcttca cgagttttca
     3781 ctaactgcgc gagatcgacg cgagcttccg ggacgtccgg gtccgggcac acgctgatgt
     3841 ggcccagctc gttaacgtca tagtagttat tgccccacca ggcaatattg taagtacgca
     3901 gcatcttgct ggcttcctgg gagctcattg caacctcctg catggagcgt agtacaccgt
     3961 gttcgcccgc tgacgaaggc aaacccatag acatgtcgtc agacatagcg aacctcaaat
     4021 tattttatta agtgtaaaac agttaacgac tatcgcagcc tggaactgcg ataacaaccc
     4081 ataagcacat ggattttcca gcagtgtatg ctgacgctcc gactgcgcaa ccggtttctt
     4141 tttcataaca ttattaagca cataaccgaa cgtaagtgtg acagttcggc gaaaccacga
     4201 gaaaactctt gtttttacaa gagcgccctt gttcagtcct cagtaactgt aaccagctct
     4261 tgaatcctga gaagcgccga gatgggtata acatcggcag gtatgcaaag cagagatgca
     4321 gagtgcgggg aacgaatctt caccagaacg gtgagacagg ttaagcagca gacaacggtt
     4381 cattatttcg tatcacctcc acggccgcct gttaagacga acccacaagc caaaactctg
     4441 atttcaaccc ggctggaagt ggcaacacga aagaaacgtc gtgtgctttt tatttaagcc
     4501 gcgcgccgcg ttttataccc cacaatggca gaaaattgca aaagataaat acgcagaatg
     4561 ccggcattgt caggaaaaat ttccagccac gtttttaaca gaatgagaca cgattcaaaa
     4621 aaaagtggaa atcgggtgaa gaattgacct aaaatagcca tccagatgtt aatccatcca
     4681 taccgattaa cactcagact gccagtgttt ttaacttgca gagtcgtggt aggatccgct
     4741 accacagaaa atccacacaa cagtttgagc taaccaaatt ctctttaggt gatattaaat
     4801 atggcaaaac acctttttac gtccgagtcc gtctctgaag ggcatcctga caaaattgct
     4861 gaccaaattt ctgatgccgt tttagacgcg atcctcgaac aggatccgaa agcacgcgtt
     4921 gcttgcgaaa cctacgtaaa aaccggcatg gttttagttg gcggcgaaat caccactagc
     4981 gcctgggtag acatcgaaga gatcacccgt aacaccgttc gcgaaattgg ctatgtacat
     5041 tccgacatgg gctttgacgc taactcctgt gcggttctga gcgctatcgg caaacagtct
     5101 cctgacatca accagggcgt tgaccgtgcc gatccgctgg aacagggcgc gggtgaccag
     5161 ggtctgatgt ttggctacgc aactaatgaa accgacgtgc tgatgccagc acctatcacc
     5221 tatgcacacc gtctggtaca gcgtcaggct gaagtgcgta aaaacggcac tctgccgtgg
     5281 ctgcgcccgg acgcgaaaag ccaggtgact ttccagtatg acgacggcaa aatcgttggt
     5341 atcgatgctg tcgtgctttc cactcagcac tctgaagaga tcgaccagaa atcgctgcaa
     5401 gaagcggtaa tggaagagat catcaagccg attctgcccg ctgaatggct gacttctgcc
     5461 accaaattct tcatcaaccc gaccggtcgt tttgttatcg gtggcccgat gggtgactgc
     5521 ggtctgactg gtcgtaaaat tatcgttgat acctacggcg gcatggcacg tcacggtggc
     5581 ggagcattct ctggtaaaga tccatcaaaa gtggaccgtt ccgctgcgta cgcagcacgt
     5641 tatgttgcaa aaaacatcgt tgctgctggc ctggccgatc gttgtgaaat tcaggtttcc
     5701 tacgcaatcg gcgtggctga accgacctcc atcatggtag aaactttcgg taccgagaaa
     5761 gtgccttctg aacaactgac tctgctggta cgtgagttct tcgacctgcg cccatacggt
     5821 ctgattcaga tgctggatct gctgcacccg atctacaaag aaaccgcagc atacggtcac
     5881 tttggtcgtg aacatttccc gtgggaaaaa accgacaaag cgcagctgct gcgcgatgct
     5941 gccggtctga agtaatcttt cttcacctgc tttcaaaggc cagcctcgcg ctggcctttt
     6001 tcttttacct caccgcggtg gataggcatt cacgccgcat ccggcaaaaa gctgcccgca
     6061 caataacatc attcttcctc aacacgtttt atcgcaaatt atcatcacta ctgaaaccga
     6121 ttacaccaat aacaccagcc aaatatttgt aatattttca tattattatt cagtttt
//
LOCUS       DAZKHF020000254         6141 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2201S31241
            SAMN43267800-rid25106413.denovo.256, whole genome shotgun sequence.
ACCESSION   DAZKHF020000254 DAZKHF020000000
VERSION     DAZKHF020000254.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267800
            Sequence Read Archive: SRR30307263
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6141)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6141)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 6141)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350581.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350581.2
            Genome Coverage       :: 151x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:21:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,652
            CDSs (total)                      :: 5,557
            Genes (coding)                    :: 5,320
            CDSs (with protein)               :: 5,320
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 237
            CDSs (without protein)            :: 237
            Pseudo Genes (ambiguous residues) :: 0 of 237
            Pseudo Genes (frameshifted)       :: 102 of 237
            Pseudo Genes (incomplete)         :: 130 of 237
            Pseudo Genes (internal stop)      :: 52 of 237
            Pseudo Genes (multiple problems)  :: 41 of 237
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6141
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267800-rid25106413.denovo.256"
                     /serotype="O 26:H11"
                     /isolate="2201S31241"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-01"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            108..242
                     /locus_tag="ACHOSC_RS20165"
                     /old_locus_tag="pgaptmp_004033"
     CDS             108..242
                     /locus_tag="ACHOSC_RS20165"
                     /old_locus_tag="pgaptmp_004033"
                     /inference="COORDINATES: protein motif:HMM:NF012798.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4549863.1"
                     /translation="MPHPERNHCLLLMGYLHGVQASELLGIKLSDIDLQAGNLNIRRL
                     "
     gene            843..1664
                     /locus_tag="ACHOSC_RS20170"
                     /old_locus_tag="pgaptmp_004034"
     CDS             843..1664
                     /locus_tag="ACHOSC_RS20170"
                     /old_locus_tag="pgaptmp_004034"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001302199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HGU4549864.1"
                     /translation="MLNARHLPVLMYHHVSRCPGLVTLSPETFCEQMKWLADNNWRTV
                     TSAELEYFYQGGTLPRKSVMLTFDDGYLDNWLRAWPVLKEYNLHAHIFLITGLIGKGN
                     VRSRQEQEYSHRDCERLIADNRSDEVMLRWSEVREMRDSGLVEFHVHTHSHKRWDRLS
                     VSRAEQCRLMKEDILVGKQCLTEKLGFCSSHLCWPEGYYNRDYINLAGKLGFSYLYTT
                     ERRMNCPENGSLRIGRISTKEREHSGWLKRRLFYYTTPLFSSVLALHKGPRLPDN"
     gene            1664..2770
                     /locus_tag="ACHOSC_RS20175"
                     /old_locus_tag="pgaptmp_004035"
     CDS             1664..2770
                     /locus_tag="ACHOSC_RS20175"
                     /old_locus_tag="pgaptmp_004035"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HGU4549865.1"
                     /translation="MMKILFTESSSDIGGQELQALAQMTALQKQGHSVLLACREKSKI
                     APEARKRGHDVTFIPFRNSLHLPSILRLRRIIGEFKPDLVICHSGHDSNIAGLSRLIC
                     CHRFSIVRQKTYITRKTRTFSLNYLCDFIVVPSSAMMAHLMAEGVRTPVTVIPPGFDW
                     PALHNEAMRPLPLHIHAWAASADNVPLIVQVGMLRPEKGHEFMLRVLYQLKMEGKSFR
                     WLVVGAGREEYEARLRQQTEHLGMSGDVLMAGALFPALPVYRIASVVVMPSENEAFGM
                     VLAEASVSGVPVIASETGGIPDVIQKNVTGTLLPVGDVSAWTGALRDFLSRPERFRMM
                     AASAREDIEYRFDINRTAQIIVSLASQAKGKCNR"
     gene            2864..4585
                     /gene="cptA"
                     /locus_tag="ACHOSC_RS20180"
                     /old_locus_tag="pgaptmp_004036"
     CDS             2864..4585
                     /gene="cptA"
                     /locus_tag="ACHOSC_RS20180"
                     /old_locus_tag="pgaptmp_004036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoethanolamine transferase CptA"
                     /protein_id="HGU4549866.1"
                     /translation="MHLNTGQNRPTFSWSALGWAIFYFGFFSTLLQVIIFSSGYSGTN
                     GIRDSLLFSCLWLIPVFLYPDRIKIIAAVVGFILWGTSLAALCYYFLYGHEFSQSVLF
                     VMFETNAREAGEYFSQYFSLKLLLISLVYTAVSVFLWTRLRPVYIPLPWRRIVSFLLL
                     YALLLHPVVLKSLIRQEPLNDTLGKLASRMEPAAPWQFVSSYYQYHQQLNALTTFLNE
                     NSALPPLGNLRDESGERPRTLVLVIGESTQRERMSLYGYLRETTPELDALRKTDPGLT
                     VFNNVVASRPYTIEALQQALTFANEKNPDLYLTQPSLMNMMKQAGYKTFWITNQQTIT
                     ARNTMLTVFSRQTDRQYYMNQQRTQSAREYDTNVLKPFREVLNDPAPKKLIIVHLLGT
                     HIKYKYRYPEGQGRFDGITGHIPTGLNAKELEVYNDYDNANLFNDHVVASLIKDFRAT
                     APDGFLLYFSDHGEEVYDTPPYKTQGRNEDNPTRPMYTVPFLLWTSEKWHAAHPRDFS
                     QYVDRKYSLAELIHTWSDLAGLTYDGYDPTRSLVNPQFRETTRWIGNPYKKNGLTDFD
                     TLPYGEP"
     gene            4659..5657
                     /gene="lpxM"
                     /locus_tag="ACHOSC_RS20185"
                     /old_locus_tag="pgaptmp_004037"
                     /gene_synonym="msbB"
     CDS             4659..5657
                     /gene="lpxM"
                     /locus_tag="ACHOSC_RS20185"
                     /old_locus_tag="pgaptmp_004037"
                     /gene_synonym="msbB"
                     /EC_number="2.3.1.243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052689.1"
                     /note="LpxM is lauroyl-Kdo(2)-lipid IV(A)
                     myristoyltransferase, an enzyme characterized in
                     Escherichia coli and involved in biosynthesis of the form
                     of lipid A found in that species and some closely related
                     species; Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009276 - Gram-negative-bacterium-type
                     cell wall [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lauroyl-Kdo(2)-lipid IV(A) myristoyltransferase"
                     /protein_id="HGU4549867.1"
                     /translation="MVPPSAVLCYHNEISRQIPVNMKNIRTEFIPRFNLTLCFPRYWM
                     TWTGIGIICVFAMVPPALRDPLLGKLGMLVGRLGKSARQRALINLSLCFPEYSDKEKE
                     NIVDAMFATASMAVVLMAELALSGPDKISHRIRWNGLEIVEKMAQNNEKVIFLVPHAW
                     GVDIPAMLIAASGRKMAAMFHNQRNPVVDYVWNSVRRRFGGKLHARNDGIASFVRSVR
                     QGYWGYYLPDQDHGPEFSEFADFFATYKATLPVIGRLSRISGARIIPLFPVYDGKTHH
                     LTIHVSPPLAIRQKSDAHIARQINEVVENFVRPHPEQYTWILKLLKTRKEGEEDPY"
     gene            5710..5898
                     /locus_tag="ACHOSC_RS20190"
                     /old_locus_tag="pgaptmp_004038"
                     /pseudo
     CDS             5710..5898
                     /locus_tag="ACHOSC_RS20190"
                     /old_locus_tag="pgaptmp_004038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012767718.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(5961..>6141)
                     /locus_tag="ACHOSC_RS20195"
                     /old_locus_tag="pgaptmp_004039"
     CDS             complement(5961..>6141)
                     /locus_tag="ACHOSC_RS20195"
                     /old_locus_tag="pgaptmp_004039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000998048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="transposase domain-containing protein"
                     /protein_id="HGU4549868.1"
                     /translation="FGSDHGGERGALLYSLIGTCKLNDVDPESYLRHVPGVIADWPVN
                     RVSELLPWRIALPAE"
BASE COUNT         1561 a         1353 c         1559 g         1668 t
ORIGIN      
        1 cagcttttta aactaatatc gactttcaat aaataaggac aggtaatgga caacaaaaat
       61 atctgaaccc gggtgagatt cataagttgc acagtgctgt cttcaaagtg ccacacccgg
      121 aacgtaatca ctgcctgctc cttatggggt atcttcatgg tgtacaagca tcagagttat
      181 taggtataaa actgtctgat attgatttgc aggccggtaa cctgaatatc cggcggctct
      241 aaacggtttg tcaactcagc ccctcggctg aaacaggagg tacagtccat tcgtcgctgg
      301 ctgaaacttc gggatgaatg ggtagaagag ggatatgaat ggttatttaa cctctatggc
      361 aggtctgcta caggtattac tgttgttctg gggggcactg gtgtggttaa tatctacgat
      421 gcaagagatt ctcacgatgg cccggtccat cagtgcatgc ggcgcgatat tgtgcgagta
      481 tttttcaggg ggagtggtat ccggatgcag aaaattccgc tactcgactg gttttatgca
      541 ataaatcagg atgataatct tctgattttt tactgaggcg gatatctctc gctgtgttta
      601 ctgaaattgc ggtattctgt tttttctgaa aaacagaaaa tctgattaaa aagagcatat
      661 gcgttaaggg aaatttaagt tctggttgat taactgataa aaatattaaa aaatccctgt
      721 tgtcggtaat ctccggacca ttccgggatt acacaataat atcaccatgt gtgatatcta
      781 tggatcctca ctgttgttta cgcgaggata ttttcagaat gcttaacaga cgataatttt
      841 ttatgttgaa tgcaaggcat cttcccgtac tgatgtatca tcatgtcagt cgttgtccgg
      901 ggcttgtgac actctcacct gaaacatttt gtgaacagat gaagtggctc gcagataata
      961 actggcggac ggtaacatct gctgagcttg aatattttta tcagggggga acgcttccac
     1021 gaaagagtgt catgctcaca ttcgatgatg gttatcttga taactggctc agagcatggc
     1081 cggtcctgaa ggaatataac cttcatgcac atatttttct cattacgggg ctgattggta
     1141 agggaaacgt acgtagcagg caggagcagg aatattctca ccgcgactgt gaacggttga
     1201 ttgccgataa ccgttcagat gaggtcatgc ttcgctggtc tgaagtccgg gaaatgcgtg
     1261 attccggact ggttgaattt catgtgcaca cccattcaca taaacgctgg gacaggttgt
     1321 ctgtatcacg tgctgaacag tgccggctca tgaaagaaga tattctggtg ggaaaacaat
     1381 gtctgacgga aaagctgggg ttctgtagtt cccatctgtg ctggcctgaa ggatattata
     1441 atcgggatta tattaatctc gccggtaagc ttggtttctc ttatttatat acaaccgaaa
     1501 gaagaatgaa ctgtcctgaa aatggttcat tacgcatagg gcgtatcagc accaaagagc
     1561 gggaacattc cggctggctg aaacgacgcc ttttttatta tacaacgcca cttttttctt
     1621 cagtactggc tcttcataag gggccaagat taccagataa ttaatgatga aaattttatt
     1681 cactgaatct tcgtcagata ttgggggcca ggagctgcag gcactggccc agatgactgc
     1741 tcttcaaaag cagggacatt ccgttttact tgcctgcagg gaaaaaagta aaattgctcc
     1801 ggaagccaga aaaagagggc atgatgtcac ttttattcct ttcaggaaca gccttcacct
     1861 tccttccatc ctcagacttc gacggattat cggggaattt aaacctgatc tggtcatctg
     1921 ccatagtggc catgacagta atatagcagg actgagccgt ctgatatgct gtcaccgctt
     1981 cagtattgtc agacaaaaaa cctacatcac ccgtaagacc cgaacgtttt ccctgaatta
     2041 tctgtgtgat ttcattgttg tgcctagctc agccatgatg gcgcatctga tggcggaagg
     2101 tgtcaggaca ccggtaacgg ttattccacc gggtttcgac tggcccgcat tgcataatga
     2161 agccatgcga ccgttacccc tgcatatcca cgcctgggct gcatcagcag acaatgttcc
     2221 gcttatcgtt caggtgggga tgctacgtcc cgaaaaagga catgaattta tgctgcgtgt
     2281 attgtatcag ctgaagatgg aagggaaatc atttcgctgg ctcgtggtgg gcgcggggcg
     2341 tgaagaatac gaagcacgtc tgcgtcagca gacagaacac ctgggaatga gcggagatgt
     2401 gttaatggca ggtgcccttt tcccagcgtt accggtctac cggattgcca gtgtggtggt
     2461 catgccttcg gaaaatgaag cgtttggtat ggtactggct gaagcgtcag tctctggcgt
     2521 gcccgttatc gccagtgaga cgggcgggat ccctgatgtc attcagaaga atgtgacagg
     2581 gacgctgtta cccgtggggg atgtgtctgc atggaccggc gcactgcgcg attttttatc
     2641 ccgaccggaa cgcttccgga tgatggcagc cagcgccaga gaagatattg agtaccggtt
     2701 tgatattaac agaacggcac agatcattgt gtctctggct agtcaggcta aggggaaatg
     2761 taacagataa cggcggatcc tcttttcagc ccgaattttg ttcagcggtt ttccggcaag
     2821 taacggagta atttatcaca ttgtttaaat acggggacgg tcaatgcatt taaataccgg
     2881 acagaacagg cctactttca gctggagtgc gctgggatgg gcaatttttt attttggctt
     2941 tttttccact ctcctgcagg tcatcatctt cagcagtggg tacagcggaa cgaatggaat
     3001 acgggactca ctgttattca gttgtctgtg gttgatcccg gtgtttctct atcctgatcg
     3061 gataaaaata attgcagctg ttgtcggttt cattctctgg ggcacgtcgc tggcagcact
     3121 ttgttattat tttctctatg gtcatgaatt ctctcaaagt gttcttttcg ttatgtttga
     3181 aacgaatgcc agagaggctg gtgaatattt cagccagtat ttcagcctta aactgttgct
     3241 tatctcgctg gtatatactg cggtgtccgt ttttctgtgg acacgtctgc gtcctgtata
     3301 tattcctttg ccatggcgga gaattgtctc tttcctgctg ctttatgctc tgcttctgca
     3361 tccggttgtt ctgaaatcgt taatcagaca ggagccgctg aatgatactc taggcaaact
     3421 ggcatcccga atggagcctg ctgccccctg gcagtttgta tccagttatt atcagtacca
     3481 tcagcaactg aatgcactga caaccttcct gaatgaaaat agcgcactgc caccactggg
     3541 taatctcagg gatgaatcag gggagagacc acgcacactg gtcctggtga ttggtgagtc
     3601 gacacagcgc gaacgcatga gcttatacgg gtatctacgt gaaacgacgc cggagctgga
     3661 tgcactgcgt aaaaccgatc cgggtcttac tgtgtttaat aatgtggtgg catcgcgtcc
     3721 gtacaccatt gaagcattgc aacaggccct tactttcgcc aacgaaaaga atcctgatct
     3781 gtatctgacg cagccgtcgc tgatgaacat gatgaagcag gcaggctata aaacattctg
     3841 gattaccaac cagcagacaa tcacagcccg taacaccatg ctcactgtat tttcgcgcca
     3901 gacggacagg cagtactaca tgaatcagca gcgaacacaa agtgcgcgtg aatatgacac
     3961 taacgtgctg aagccgttcc gggaagtgct gaatgaccct gcaccaaaga aactgatcat
     4021 cgtgcatctg ctgggtacgc acattaagta taaataccgt tatccggaag gtcaggggcg
     4081 gtttgatggc attacagggc atattcccac tggattaaat gcgaaagagc tggaagtgta
     4141 taacgattac gataatgcca atctgtttaa cgatcatgtg gtggccagtc tgataaagga
     4201 cttcagagca actgcgccgg acggctttct gctttatttt tcagatcatg gtgaagaagt
     4261 atatgatact ccgccatata aaacgcaggg acggaatgaa gataacccca cacgtcccat
     4321 gtacactgtt ccgttcctgc tgtggacctc ggaaaagtgg catgctgcac atccgcgaga
     4381 tttttcgcag tatgttgacc gcaaatacag tctggctgaa ctgatccaca cctggtcaga
     4441 tttggcggga ctgacatacg atggttacga tccgacccgt tctctggtga atccgcaatt
     4501 cagggaaact acccgctgga ttggaaatcc gtataaaaag aatgggctca ctgatttcga
     4561 cactcttccg tatggtgagc catagtattt tcttatcttc ggttccgttc tgaaggcagg
     4621 aggatgtgtt ttcctctgct gtccggtgga ctccagggat ggtaccacct tctgctgtac
     4681 tctgttatca taatgagata agcaggcaga taccggtaaa tatgaaaaat atcagaactg
     4741 agtttattcc ccgctttaac cttacattat gcttcccccg ttactggatg acgtggacag
     4801 gtatcggtat tatctgtgtg tttgcaatgg tcccccccgc actgagagat ccattgttgg
     4861 gaaaattggg catgctggtt gggcgtctgg ggaaaagcgc acgtcaacgg gccttgatta
     4921 atttatcgct ttgttttccg gagtacagtg ataaagaaaa ggagaatatc gttgatgcca
     4981 tgtttgcaac agcctccatg gccgttgtgc ttatggctga attagcactg tccgggcctg
     5041 ataaaatttc gcatcgtata cgctggaatg gtctggagat tgtggagaag atggcacaga
     5101 acaatgaaaa ggtcattttt cttgtgccac atgcctgggg agtggatatt cccgcaatgc
     5161 tgatagctgc gtcgggcaga aaaatggcag cgatgtttca taaccagaga aatccggtgg
     5221 tggattatgt ctggaacagt gttcgtcgtc gtttcggagg aaaactgcac gcacgtaatg
     5281 acggtattgc ctcctttgtc cgctcagtcc ggcagggata ctggggatat tatcttcctg
     5341 atcaggatca tggcccggaa ttcagtgagt ttgcagattt ttttgcgacg tataaagcaa
     5401 ctttaccggt tatcggacgg ctcagcagga tcagtggtgc acggataata ccgcttttcc
     5461 ctgtttatga cggaaagacc catcatctga ctattcatgt cagcccgcca cttgctatac
     5521 gacagaagag tgatgctcat attgcgcgac agataaacga agtggttgaa aatttcgttc
     5581 gtccacatcc tgagcagtat acgtggatcc tcaagttgct gaaaacgcgt aaagaagggg
     5641 aagaggatcc ttactgaggc aggtttttca ggataatgag cttacccagc aatagtggac
     5701 acacgctaag tgagtaaact ctcagtcaga ggtggctcat atgacaaaaa aacagtacca
     5761 accagtaaaa aaccccgtaa atagcattcg cctgaatttc gcagtgaagc cctgaagctt
     5821 gctgaacgca tcggtgttgc tgtcgcagcc cgtgtgctca gtctgtatga atcacagctt
     5881 gtaagcggct cgtcagaact gtattgatat ttactgagcg gtcgtaagcg tacagcgagg
     5941 gccgtattga cggggatgtg ttattcagct ggcagtgcta tgcgccacgg aagcagttcg
     6001 ctgacccggt tgaccggcca gtctgctatg acgccaggca catggcgaag gtagctttct
     6061 ggatccacgt cattcagttt gcacgtcccg atcaggctgt acagtagcgc tccccgctca
     6121 ccaccatggt cagagccgaa g
//
LOCUS       DAZKHF020000255         6115 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2201S31241
            SAMN43267800-rid25106413.denovo.257, whole genome shotgun sequence.
ACCESSION   DAZKHF020000255 DAZKHF020000000
VERSION     DAZKHF020000255.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267800
            Sequence Read Archive: SRR30307263
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6115)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6115)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 6115)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350581.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350581.2
            Genome Coverage       :: 151x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:21:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,652
            CDSs (total)                      :: 5,557
            Genes (coding)                    :: 5,320
            CDSs (with protein)               :: 5,320
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 237
            CDSs (without protein)            :: 237
            Pseudo Genes (ambiguous residues) :: 0 of 237
            Pseudo Genes (frameshifted)       :: 102 of 237
            Pseudo Genes (incomplete)         :: 130 of 237
            Pseudo Genes (internal stop)      :: 52 of 237
            Pseudo Genes (multiple problems)  :: 41 of 237
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6115
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267800-rid25106413.denovo.257"
                     /serotype="O 26:H11"
                     /isolate="2201S31241"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-01"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            48..824
                     /gene="exuR"
                     /locus_tag="ACHOSC_RS20200"
                     /old_locus_tag="pgaptmp_004040"
     CDS             48..824
                     /gene="exuR"
                     /locus_tag="ACHOSC_RS20200"
                     /old_locus_tag="pgaptmp_004040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator ExuR"
                     /protein_id="HGU4549869.1"
                     /translation="MEITEPRRLYQQLAADLKERIEQGVYLVGDKLPAERFIADEKNV
                     SRTVVREAIIMLEVEGYVEVRKGSGIHVVSNQPRHQQAADNNMEFANYGPFELLQARQ
                     LIESNIAEFAATQVTKQDIMKLMAIQEQARGEQCFRDSEWDLQFHIQVALATQNSALA
                     AIVEKMWTQRSHNPYWKKLHEHIDSRTVDNWCDDHDQILKALIRKDPHAAKLAMWQHL
                     ENTKIMLFNETSDDFEFNADRYLFAENPVVHLDTATSGSK"
     gene            1064..1147
                     /gene="yqiM"
                     /locus_tag="ACHOSC_RS20205"
                     /old_locus_tag="pgaptmp_004041"
     CDS             1064..1147
                     /gene="yqiM"
                     /locus_tag="ACHOSC_RS20205"
                     /old_locus_tag="pgaptmp_004041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YqiM"
                     /protein_id="HGU4549870.1"
                     /translation="MLMYQTRRTYQNSNNIAVVHLLKPAWR"
     gene            1169..1831
                     /gene="yqjA"
                     /locus_tag="ACHOSC_RS20210"
                     /old_locus_tag="pgaptmp_004042"
     CDS             1169..1831
                     /gene="yqjA"
                     /locus_tag="ACHOSC_RS20210"
                     /old_locus_tag="pgaptmp_004042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family general envelope maintenance protein
                     YqjA"
                     /protein_id="HGU4549871.1"
                     /translation="MELLTQLLQALWAQDFETLANPSMIGMLYFVLFVILFLENGLLP
                     AAFLPGDSLLVLVGVLIAKGAMGYPQTILLLTVAASLGCWVSYIQGRWLGNTRTVQNW
                     LSHLPAHYHQRAHHLFHKHGLSALLIGRFIAFVRTLLPTIAGLSGLNNARFQFFNWMS
                     GLLWVLILTTLGYMLGKTPVFLKYEDQLMSCLMLLPVVLLVFGLAGSLVVLWKKKYGN
                     RG"
     gene            1835..2218
                     /gene="mzrA"
                     /locus_tag="ACHOSC_RS20215"
                     /old_locus_tag="pgaptmp_004043"
     CDS             1835..2218
                     /gene="mzrA"
                     /locus_tag="ACHOSC_RS20215"
                     /old_locus_tag="pgaptmp_004043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EnvZ/OmpR regulon moderator MzrA"
                     /protein_id="HGU4549872.1"
                     /translation="MQIPRMSLRQLAWSGTVLLLVGTLLLAWSAVRQQESTLAIRAVH
                     QGTTMPDGFSIWHHLDAHGIPFKSITPKNDTLLITFDSSDQSAAAKAVLDRTLPHGYI
                     IAQQDNNSQAMQWLTRLRDNSHRFG"
     gene            2365..2733
                     /gene="yqjC"
                     /locus_tag="ACHOSC_RS20220"
                     /old_locus_tag="pgaptmp_004044"
     CDS             2365..2733
                     /gene="yqjC"
                     /locus_tag="ACHOSC_RS20220"
                     /old_locus_tag="pgaptmp_004044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417568.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1090 family protein YqjC"
                     /protein_id="HGU4549873.1"
                     /translation="MKYRIALAVSLFALSAGSYATTLCQEKEQNILKEISYAEKHQNQ
                     NRIDGLNKALSEVRANCSDSQLRADHHKKIAKQKDEVAERQQDLAEAKQKGDADKIAK
                     RERKLAEAQEELKKLEARDY"
     gene            2771..3076
                     /gene="yqjD"
                     /locus_tag="ACHOSC_RS20225"
                     /old_locus_tag="pgaptmp_004045"
     CDS             2771..3076
                     /gene="yqjD"
                     /locus_tag="ACHOSC_RS20225"
                     /old_locus_tag="pgaptmp_004045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YqjD family protein"
                     /protein_id="HGU4549874.1"
                     /translation="MSKEHTTEHLRAELKSLSDTLEEVLSSSGEKSKEELSKIRSKAE
                     QALKQSRYRLGETGDAIAKQTRVAAARADEYVRENPWTGVGIGAAIGVVLGVLLSRR"
     gene            3079..3483
                     /gene="yqjE"
                     /locus_tag="ACHOSC_RS20230"
                     /old_locus_tag="pgaptmp_004046"
     CDS             3079..3483
                     /gene="yqjE"
                     /locus_tag="ACHOSC_RS20230"
                     /old_locus_tag="pgaptmp_004046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin family protein"
                     /protein_id="HGU4549875.1"
                     /translation="MADTHHAQGPGKSVLGIGQRIVSIMVEMVETRLRLAVVELEEEK
                     ANLFQLLLMLGLTMLFAAFGLMSLMVLIIWAVDPQYRLNAMIATTVVLLLLALIGGIW
                     TLRKSRKSTLLRHTRHELANDRQLLEEESREQ"
     gene            3473..3772
                     /gene="yqjK"
                     /locus_tag="ACHOSC_RS20235"
                     /old_locus_tag="pgaptmp_004047"
     CDS             3473..3772
                     /gene="yqjK"
                     /locus_tag="ACHOSC_RS20235"
                     /old_locus_tag="pgaptmp_004047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqjK-like family protein"
                     /protein_id="HGU4549876.1"
                     /translation="MSSKVERERRKAQLLSQIQQQRLDLSASRREWLEATGAYDRRWN
                     MLLSLRSWALVGSSVMAIWTIRHPNMLVRWARRGFGVWSAWRLVKTTLKQQQLRG"
     gene            3958..4350
                     /gene="yqjF"
                     /locus_tag="ACHOSC_RS20240"
                     /old_locus_tag="pgaptmp_004048"
     CDS             3958..4350
                     /gene="yqjF"
                     /locus_tag="ACHOSC_RS20240"
                     /old_locus_tag="pgaptmp_004048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008503138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DoxX family protein"
                     /protein_id="HGU4549877.1"
                     /translation="MKKLEDVGVLVARILMPILFITAGWGKITAYSGTQQYMEAMGVP
                     GFMLPLVILLEFGGGLAILFGFLTRTTALFTAGFTLLTAFLFHSNFAEGVNSLMFMKN
                     LTISGGFLLLAITGPGAYSIDRLLNKKW"
     gene            4420..5406
                     /gene="yqjG"
                     /locus_tag="ACHOSC_RS20245"
                     /old_locus_tag="pgaptmp_004049"
     CDS             4420..5406
                     /gene="yqjG"
                     /locus_tag="ACHOSC_RS20245"
                     /old_locus_tag="pgaptmp_004049"
                     /EC_number="1.8.5.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708909.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathionyl-hydroquinone reductase YqjG"
                     /protein_id="HGU4549878.1"
                     /translation="MGQLIDGVWHDTWYDTKSTGGKFQRSASAFRNWLTADGAPGPTG
                     TGGFIAEKDRYHLYVSLACPWAHRTLIMRKLKGLEPFISVSVVNPLMLENGWTFDDSF
                     PGATGDTLYQHEFLYQLYLHADPHYSGRVTVPVLWDKKNHTIVSNESAEIIRMFNTAF
                     DALGAKAGDYYPPALQTKIDELNGWIYDTVNNGVYKAGFATSQQAYDEAVAKVFESLA
                     RLEQILGQHRYLTGNQLTEADIRLWTTLVRFDPVYVTHFKCDKHRISDYLNLYGFLRD
                     IYQMPGIAETVNFDHIRNHYFRSHKTINPTGIISIGPWQDLDEPHGRDVRFG"
     gene            5699..6064
                     /gene="yhaH"
                     /locus_tag="ACHOSC_RS20250"
                     /old_locus_tag="pgaptmp_004050"
     CDS             5699..6064
                     /gene="yhaH"
                     /locus_tag="ACHOSC_RS20250"
                     /old_locus_tag="pgaptmp_004050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF805 domain-containing protein"
                     /protein_id="HGU4549879.1"
                     /translation="MDWYLKVLKNYVGFRGRARRKEYWMFILVNIIFTFVLGLLDKML
                     GWQRAGGEGILTTIYGILVFLPWWAVQFRRLHDTDRSAWWALLFLIPFIGWLIIIVFN
                     CQAGTPGENRFGPDPKLEP"
BASE COUNT         1523 a         1515 c         1544 g         1533 t
ORIGIN      
        1 taaagtggta tgataacttc gtccgcattc cactccggga gacgcatatg gaaatcactg
       61 aaccacgccg tttgtatcaa caacttgccg ctgacctgaa agagcgcatc gaacagggcg
      121 tctatctggt aggtgataaa ctgcctgcag aacgctttat tgccgatgaa aagaacgtca
      181 gccgtacggt agttcgtgaa gccatcatca tgctggaagt tgaaggctat gtcgaagtgc
      241 gtaaaggttc aggtattcat gtggtttcca accagccgcg ccatcagcag gcggctgaca
      301 ataatatgga attcgccaat tacggtccgt ttgagttgct tcaggctcgc cagctcatcg
      361 aaagtaatat tgccgagttc gcggcaactc aggtaacgaa acaagacatc atgaaactga
      421 tggccattca ggaacaggcg cgcggcgaac aatgcttccg tgattccgag tgggatttac
      481 agttccacat tcaggtagcc ctggcgacgc agaactccgc cctggcggcc atcgttgaaa
      541 aaatgtggac ccaacgtagc cataacccgt actggaaaaa actgcacgaa cacattgatt
      601 cccgtaccgt cgataactgg tgtgatgacc acgatcaaat cctcaaggcg ctgattcgca
      661 aagatcctca tgctgctaag ctggcaatgt ggcagcacct ggaaaacacc aagatcatgt
      721 tattcaacga aaccagcgac gacttcgagt tcaatgccga ccgctatctg ttcgccgaaa
      781 acccggtagt gcatctcgat acggccacca gcggcagtaa atgaaatacc tgtccgacag
      841 gcgctgcgac gcgcctgttt gtctcgtcgg taagcgaaaa gtataaagtg tcagcctgtg
      901 taaatcctct cgccaccctc ccctgcattc agcaaaatca gactccacgg acatgtaatt
      961 ttgataacga acaacgttga cctttgttac aattagattc aatttgaatt tatgtttttg
     1021 aatgctttct tatctcacga tttaacaggg aatagttcag gctgtgttga tgtatcaaac
     1081 ccgcagaaca taccaaaaca gcaataacat tgcggtagtg catcttttaa aaccagcgtg
     1141 gcgttaaccg attcaccagg aataatgaat ggaacttttg acccaattgc tgcaagccct
     1201 gtgggcgcag gattttgaaa ccctggccaa tccatcgatg attggcatgt tgtattttgt
     1261 cttgtttgta attttgttcc ttgaaaacgg cttgcttcca gcggcctttt taccgggcga
     1321 cagtttactg gtattggtcg gcgtgttgat tgcgaaaggc gcgatgggtt acccgcaaac
     1381 gattctgctg ctgaccgttg ccgccagcct cggctgctgg gtcagctata ttcaggggcg
     1441 atggctgggc aatacccgca ccgtacaaaa ctggctatct catttacccg cgcattatca
     1501 tcaacgcgca caccatcttt ttcataaaca cggtttatcg gcgctgttaa ttggtcgctt
     1561 tattgcgttt gtcagaacac tgctgccgac gattgccggg ttatcagggc tgaataacgc
     1621 gcgctttcag tttttcaact ggatgagcgg tctgctgtgg gtattgatcc tgacaactct
     1681 gggttacatg ctcggcaaaa cgccggtatt tttaaagtac gaggaccagc tgatgtcatg
     1741 cctgatgctg ctcccggtgg tgctgctggt gtttggcctg gcaggttctc tggtcgtgtt
     1801 atggaaaaag aaatatggaa atcgggggta agggatgcaa atacctcgca tgtcgcttcg
     1861 ccagctagcc tggtccggca ctgttttact tctggtcggc acgctgttgc tggcctggtc
     1921 cgcggttcgc cagcaagagt ctacgctggc gattcgtgcc gttcatcaag gcacaacgat
     1981 gccagacggt ttttcgatct ggcatcacct tgacgctcat ggcattcctt tcaaaagtat
     2041 cacccccaaa aacgacactc tgttaattac atttgactcc agcgaccaga gcgccgccgc
     2101 aaaagcggtc ctcgacagaa cattgcccca tggctacatc attgcgcagc aggacaataa
     2161 cagtcaggct atgcagtggc tgacccggtt acgggataac tctcatcgct tcggataact
     2221 tcctgttatc cgaaacatat cactcacttt ggtgatttca ccgtaactgt ctatgattaa
     2281 tgaggcggtg gttaacacta ccgcttcgtt gtctggacaa tcgttccttt gtaataggtc
     2341 cgaatcacaa tggaaggttc aagaatgaaa taccgcatcg ctttagctgt ttctctcttt
     2401 gctcttagtg ccggtagtta tgccactacc ctgtgtcagg aaaaggagca aaatatcctt
     2461 aaggagatca gctatgccga aaaacaccaa aaccagaatc gtattgacgg tctgaataaa
     2521 gccctgagtg aagtccgggc caactgttca gatagccagc tgcgtgccga tcatcacaag
     2581 aaaatcgcaa agcagaaaga tgaggtggcg gaacgccagc aagatttagc cgaggcgaag
     2641 caaaaaggcg atgccgataa gattgccaaa cgcgaacgga aactggcaga agcgcaggaa
     2701 gagctgaaaa agctggaagc gcgcgactac taactcacaa tagtcactac ttactcacct
     2761 ggagaaaact atgtcgaaag aacacactac ggaacatctg cgtgctgagt tgaaatccct
     2821 ttccgatacg ctggaagagg tgcttagctc atctggcgag aagtcgaaag aagagttgag
     2881 taagattcgt agcaaagcgg agcaggcact gaaacagagc cgttatcgcc tgggtgaaac
     2941 cggtgatgcc atcgccaaac aaacccgtgt cgcggcggcg cgtgctgatg agtatgtgcg
     3001 tgaaaatccg tggaccggcg tgggcattgg cgctgcaatc ggtgtagtgc tcggcgttct
     3061 gctgtcgcgt cgttaattat ggcggacact catcacgcgc aagggcccgg taaaagcgtt
     3121 ctgggcatcg ggcagcgaat tgtttccatc atggttgaaa tggtggaaac acgtctgcgg
     3181 ctggcggtgg tggagctgga agaggaaaaa gctaatctct ttcaactttt actgatgctg
     3241 ggcctgacga tgcttttcgc tgcatttggt cttatgagcc tgatggtgct aattatttgg
     3301 gcggttgatc cgcaatatcg cctgaatgcg atgattgcca ccaccgtggt gttgctgcta
     3361 ctggcactaa ttggcggtat ctggacgtta cgtaaatcgc gtaaatccac cttgctgcgc
     3421 catacacgcc atgagctggc aaacgatcgg caactgctcg aggaggagtc ccgtgagcag
     3481 taaagtcgaa cgtgaacgac gtaaggcgca actgcttagc cagatccagc aacaacggct
     3541 ggatctttcc gccagtcgtc gtgaatggct ggaggcaaca ggcgcttacg atcgtcgctg
     3601 gaatatgctg ctaagtctgc gctcctgggc gctggttggc agtagcgtga tggcgatctg
     3661 gacgattcgc catcctaata tgctggtccg ctgggccaga cgcggttttg gcgtatggag
     3721 cgcctggcgt ctggttaaaa cgaccctcaa gcagcaacag cttcgcggtt aacttccctc
     3781 tggccggagc aattccggcc ttatccctca aatttcttga agatttttga cagttttcct
     3841 tgctaacaat catcattcac cacgtttatg attctctcca tcgacagcaa cgacgctaat
     3901 accgcgccct tgcacaaaaa aacaatcagc agcctgagtg gcgcagtgga gagtatgatg
     3961 aaaaaattag aagatgttgg tgtactggta gcgcgcattt taatgccgat tctgtttatt
     4021 accgcaggct ggggaaaaat caccgcttac tctggtaccc aacaatatat ggaagcaatg
     4081 ggtgtcccgg gttttatgct gccgctggtg attctgcttg agttcggtgg tggtctggca
     4141 atcctgttcg gtttcctgac tcgcaccaca gccctgttta ctgcgggctt tacgctgctg
     4201 acggcatttt tatttcacag caactttgct gaaggcgtca actcgctgat gttcatgaaa
     4261 aacctgacaa tttctggcgg attcctgctg ctggcaatta ccggtccggg cgcgtatagc
     4321 atcgaccgcc tgctgaataa aaagtggtaa gcacgcgcta tacttaacga taaaaaacga
     4381 ggaggaagct cctcgttttt gctattggag gagagaaaaa tggggcaact gattgacggc
     4441 gtctggcatg acacctggta cgataccaaa tctaccggcg gtaaatttca acgttcagct
     4501 tccgcatttc gtaactggct cactgccgat ggcgcacctg gccccactgg cacaggcggt
     4561 tttatcgcag agaaagatcg ttatcatctc tatgtttcac tcgcctgccc gtgggcgcac
     4621 cgcacgctga tcatgcgcaa actcaaagga ctggaaccgt ttatttccgt ttccgtagtg
     4681 aacccgctga tgctggaaaa cggctggacc tttgatgaca gttttccggg agcaaccgga
     4741 gatacgctct atcaacatga atttctgtat cagctttatc tccacgccga tccacactac
     4801 agcggacgag ttactgttcc cgtgctgtgg gacaaaaaga accacaccat cgtcagcaac
     4861 gaatcagcag aaatcatccg catgttcaat accgcgtttg atgcgctggg cgcaaaagcg
     4921 ggtgattact acccaccagc cctgcaaacg aaaattgacg aacttaacgg ctggatttat
     4981 gacaccgtta acaacggcgt gtataaagct ggttttgcca ccagccagca agcctacgac
     5041 gaggcggtgg cgaaagtgtt tgaatcgctg gcgcgactgg agcagatttt aggccagcat
     5101 cgttacctga ccggcaacca gctaaccgaa gccgatattc gcctgtggac cacgctggtc
     5161 cgttttgatc cagtgtatgt gacccacttc aagtgtgata agcaccgcat cagcgattac
     5221 cttaatctgt atggcttcct gcgtgatatc taccagatgc cgggaattgc cgaaacagtc
     5281 aatttcgatc acatccgtaa tcattacttc cgcagccata agaccatcaa ccctacgggg
     5341 attatttcaa ttggtccatg gcaggatctc gatgaaccgc atggacgaga tgttcgcttc
     5401 ggttaaaaaa aggcacctta cgaggtgcct ctgataatta gctaagtgca tgttgttcat
     5461 gtcggatgcg gcgtgaacgc cttatcaaac ctacaaaaca tttatattca atatgttgca
     5521 atgaccgcgc aggccagata agcgtagcgc atctggcgat tttgcgcttg tcatcagtct
     5581 tagcgcgcct tcacagcact ttattaattc cttgaacgaa tatttactgc catttcattc
     5641 acgtctattc ttaatttgct gctttaaaaa caagtgattg agcaaaattg aggcaaaaat
     5701 ggactggtat ctgaaagtac taaaaaatta tgtcggtttc cggggtcgtg cgcggcgcaa
     5761 agagtactgg atgtttattc tggtcaacat catctttacg ttcgtgctgg ggctgctgga
     5821 taaaatgtta ggctggcaac gcgctggtgg cgaaggcatc ctgacgacaa tctacggtat
     5881 tctggtgttt ttaccgtggt gggcagttca gttccgccgc ctgcacgaca ccgaccgctc
     5941 ggcgtggtgg gcactgttgt tcttaatccc gtttatcggc tggcttatta tcatcgtctt
     6001 taactgccag gcgggtacgc caggcgaaaa ccgctttgga ccagatccga aactggaacc
     6061 ttaaaacaaa tctgctcgcc ggatgtctga ctcatccggc ttttatcatc atgtt
//
LOCUS       DAZKHF020000256         6100 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2201S31241
            SAMN43267800-rid25106413.denovo.258, whole genome shotgun sequence.
ACCESSION   DAZKHF020000256 DAZKHF020000000
VERSION     DAZKHF020000256.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267800
            Sequence Read Archive: SRR30307263
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6100)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6100)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 6100)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350581.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350581.2
            Genome Coverage       :: 151x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:21:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,652
            CDSs (total)                      :: 5,557
            Genes (coding)                    :: 5,320
            CDSs (with protein)               :: 5,320
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 237
            CDSs (without protein)            :: 237
            Pseudo Genes (ambiguous residues) :: 0 of 237
            Pseudo Genes (frameshifted)       :: 102 of 237
            Pseudo Genes (incomplete)         :: 130 of 237
            Pseudo Genes (internal stop)      :: 52 of 237
            Pseudo Genes (multiple problems)  :: 41 of 237
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6100
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267800-rid25106413.denovo.258"
                     /serotype="O 26:H11"
                     /isolate="2201S31241"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-01"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            <1..1407
                     /gene="ydfI"
                     /locus_tag="ACHOSC_RS20255"
                     /old_locus_tag="pgaptmp_004051"
     CDS             <1..1407
                     /gene="ydfI"
                     /locus_tag="ACHOSC_RS20255"
                     /old_locus_tag="pgaptmp_004051"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol dehydrogenase family protein"
                     /protein_id="HGU4549880.1"
                     /translation="NLAPRIIHLGFGAFHRAHQGVYADILATEHFSDWGYYEVNLIGG
                     EQQIADLQQQDNLYTVAEMSADAWTARVVGVVKKALHVQMDGLETVLAAMCEPQIAIV
                     SLTITEKGYFHSPAIGQLMLDHPMVAADVQNPHQPKTATGVIVEALARRKAAGLPAFT
                     VMSCDNMPENGHVMRDVVTSYAKAVDVKLAQWIEDNVTFPSTMVDRIVPAVTEDTLAK
                     IEQLTGVGDPAGVACEPFRQWVIEDNFVAGRPEWEKAGAELVSDVLPYEEMKLRMLNG
                     SHSFLAYLGYLAGYQHINDCMEDEHYRYAAYGLMLQEQAPTLKVQGVDLQDYANRLIA
                     RYSNPALRHRTWQIAMDGSQKLPQRMLDSVRWHLAHDSKFDLLALGVAGWMRYVGGVD
                     EQGNLIEISDPLLPVIQKAVQSSAEGKARVQSLLAIKAIFGDDLPDNSLFTAKVTEAY
                     LSLLAHGAKATVAKYSVK"
     gene            complement(1443..1646)
                     /gene="ydfZ"
                     /locus_tag="ACHOSC_RS20260"
                     /old_locus_tag="pgaptmp_004052"
     CDS             complement(1443..1646)
                     /gene="ydfZ"
                     /locus_tag="ACHOSC_RS20260"
                     /old_locus_tag="pgaptmp_004052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative selenium delivery protein YdfZ"
                     /protein_id="HGU4549881.1"
                     /translation="MTTYDRNRNAITTGSRVMVSGTGHTGKILSIDTEGLTAEQIRRG
                     KTVVVEGCEEKLAPLDLIRLGMN"
     gene            complement(1824..2510)
                     /gene="rspR"
                     /locus_tag="ACHOSC_RS20265"
                     /old_locus_tag="pgaptmp_004053"
                     /gene_synonym="ydfH"
     CDS             complement(1824..2510)
                     /gene="rspR"
                     /locus_tag="ACHOSC_RS20265"
                     /old_locus_tag="pgaptmp_004053"
                     /gene_synonym="ydfH"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator rspR"
                     /protein_id="HGU4549882.1"
                     /translation="MTVETQLNPTQPVNQQIYRILRRDIVHCLIAPGTPLSEKEVSVR
                     FNVSRQPVREAFIKLAENGLIQIRPQRGSYVNKISMAQVRNGSFIRQAIECAVARRAA
                     SMITESQCYQLEQNLHQQRIAIERKQLDDFFELDDNFHQLLTQIADCQLAWDTIENLK
                     ATVDRVRYMSFDHVSPPEMLLRQHLDIFSALQKRDGDAVERAMTQHLQEISESVRQIR
                     QENSDWFSEE"
     gene            complement(2599..3345)
                     /gene="ydfG"
                     /locus_tag="ACHOSC_RS20270"
                     /old_locus_tag="pgaptmp_004054"
     CDS             complement(2599..3345)
                     /gene="ydfG"
                     /locus_tag="ACHOSC_RS20270"
                     /old_locus_tag="pgaptmp_004054"
                     /EC_number="1.1.1.298"
                     /EC_number="1.1.1.381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional NADP-dependent 3-hydroxy acid
                     dehydrogenase/3-hydroxypropionate dehydrogenase YdfG"
                     /protein_id="HGU4549883.1"
                     /translation="MIVLVTGATAGFGECITRRFIQQGHKVIATGRRQERLQELKDEL
                     GDNLYIAQLDVRNRAAIEEMLASLPAEWCNIDILVNNAGLALGMEPAHKASVEDWETM
                     IDTNNKGLVYMTRAVLPGMVERNHGHIINIGSTAGSWPYAGGNVYGATKAFVRQFSLN
                     LRTDLHGTAVRVTDIEPGLVGGTEFSNVRFKGDDGKAEKTYQNTVALTPEDVSEAVWW
                     VSTLPAHVNINTLEMMPVTQSYAGLNVHRQ"
     gene            3482..5527
                     /gene="dcp"
                     /locus_tag="ACHOSC_RS20275"
                     /old_locus_tag="pgaptmp_004055"
     CDS             3482..5527
                     /gene="dcp"
                     /locus_tag="ACHOSC_RS20275"
                     /old_locus_tag="pgaptmp_004055"
                     /EC_number="3.4.15.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidyl-dipeptidase Dcp"
                     /protein_id="HGU4549884.1"
                     /translation="MTTMNPFLVQSTLPYLAPHFDQIANHHYRPAFDEGMQQKRAEIA
                     AIALNPQTPDFNNTILALEQSGELLTRVTSVFFAMTAAHTNDELQRLDEQFSAELAEL
                     ANDIYLNGELFARVDAVWQRRESLGLDSESIRLVEVIHQRFVLAGAKLAQADKAKLKV
                     LNTEAATLTSQFNQRLLAANKSGGLVVNDIAQLAGMSEQEIALAAEAAREKGLDNKWL
                     IPLLNTTQQPALAEMRDRATREKLFIAGWTRAEKNDGNDTRAIIQRLVEIRAQQATLL
                     GFPHYAAWKIADQMAKTPEAALNFMREIVPAARQRASDELASIQAVIDKQQGGFSAQP
                     WDWAFYAEQVRREKFDLDEAQLKPYFELNTVLNEGVFWTANQLFGIKFVERFDIPVYH
                     PDVRVWEIFDHNGVGLALFYGDFFARDSKSGGAWMGNFVEQSTLNETHSVIYNVCNYQ
                     KPAAGEPALLLWDDVITLFHEFGHTLHGLFARQRYATLSGTNTPRDFVEFPSQINEHW
                     ATHPQVFARYARHYQSGAAMPDELQQKMRNASLFNKGYEMSELLSAALLDMRWHCLEE
                     NEAMQDVDDFELRALVAENMDLPAIPPRYRSSYFAHIFGGGYAAGYYAYLWTQMLADD
                     GYQWFVEQGGLTRENGLRFREAILSRGNSEDLERLYRQWRGKAPQIMPMLQHRGLNI"
     gene            complement(5572..6090)
                     /gene="ydeJ"
                     /locus_tag="ACHOSC_RS20280"
                     /old_locus_tag="pgaptmp_004056"
     CDS             complement(5572..6090)
                     /gene="ydeJ"
                     /locus_tag="ACHOSC_RS20280"
                     /old_locus_tag="pgaptmp_004056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxo-tetronate isomerase"
                     /protein_id="HGU4549885.1"
                     /translation="MNINKDKIVQLADTDTIENLTSALSQRLIADQLRLTTAESCTGG
                     KLASALCAAEDTPKFYGAGFVTFTDQAKMKILSVSQQSLERYSAVSEKVAAEMATGAI
                     ERADADVSIAITGYGGPEGGEDGTPAGTVWFAWHIKGQTYTAVMHFAGDCETVLALAV
                     RFALAQLLQLLL"
BASE COUNT         1505 a         1462 c         1577 g         1556 t
ORIGIN      
        1 aacctggccc caagaattat tcatttaggc tttggtgcat ttcaccgtgc gcatcagggc
       61 gtgtatgccg atattcttgc tacagaacat ttcagtgact ggggatatta tgaggtcaac
      121 ttaatcggcg gcgaacagca aattgccgat ttacaacagc aagataatct ttataccgtt
      181 gcggaaatgt cggccgatgc gtggacggct cgcgtcgttg gcgtcgttaa aaaagccttg
      241 cacgtacaga tggatggctt agaaaccgtg ttggctgcga tgtgtgaacc gcaaatcgcg
      301 attgtctctc tgacaatcac cgaaaaaggg tatttccact ctccggcgat cggacagtta
      361 atgctcgatc acccgatggt cgctgccgac gtgcaaaatc cccaccagcc gaaaacagca
      421 acaggagtga ttgttgaggc gctggcccgc cgtaaagcgg caggacttcc cgcatttacc
      481 gtcatgtcat gtgacaacat gccagaaaac ggtcatgtta tgcgtgacgt tgtcacttca
      541 tacgcgaaag ctgttgatgt aaaactggca caatggatcg aagataacgt gactttccca
      601 tcaacaatgg tggaccgtat tgtgcccgca gtgacagagg atacgctggc gaaaatcgaa
      661 caacttaccg gtgtgggcga tcctgcgggc gttgcctgtg aacctttccg ccagtgggta
      721 atagaagata actttgttgc cggacgtcca gaatgggaaa aagcgggagc cgaactggtt
      781 agtgatgtgc tgccttatga agagatgaag ttgcgcatgc tcaacggcag tcattcattc
      841 ctggcgtatt tgggttatct tgctggatat cagcacatta atgactgcat ggaagatgaa
      901 cattatcgtt atgcggcgta tggcttgatg ttgcaggaac aagcgccgac gctgaaagtg
      961 cagggcgttg atttgcaaga ttacgctaac cgattaattg cacgctatag caacccggcg
     1021 ttacgtcatc gaacctggca gattgcgatg gatggtagcc agaaattgcc acagcggatg
     1081 ttggattctg ttcgctggca tctggcgcat gacagcaagt tcgatctgct ggcgctgggc
     1141 gtcgcgggtt ggatgcgtta tgtcggtggt gttgatgaac agggaaatct gatagaaatc
     1201 agtgacccac tgttacctgt tattcagaag gctgtacaaa gtagtgccga agggaaagcg
     1261 cgcgtccagt cattgctggc gattaaggca atctttggtg atgatttgcc agacaatagc
     1321 ttgtttactg caaaagtgac ggaagcgtac ttgtctttat tagcgcatgg cgcgaaagcg
     1381 accgtggcga aatattccgt gaagtaaaaa aagcaatgcc gccatcggcg gcattcacac
     1441 acttagttca tgccgagacg aatcaggtcc agaggtgcca gtttctcttc acaaccttca
     1501 acaactacgg tttttccgcg gcggatttgc tcagcggtca gaccttcagt atcaatcgac
     1561 aggatcttgc cagtgtgacc ggtgccgcta accataacac ggctgccagt ggtgattgcg
     1621 ttacggttac gatcgtaggt cgtcataggt gttttctcct ttctgattta cagtcgaagc
     1681 ccacgtaacg ggcgcaatat aaatactcct gtcgagtgaa atattttttg tttttgatca
     1741 agctcacatc ttttttgatg catgcatgag ccgtaaggcg gggggaaaag cctctcatcc
     1801 cggatgggat gagagtaaat gaattactct tcgctaaacc agtcgctgtt ttcctggcgg
     1861 atctggcgca cggattcgct gatttcctgc aaatgttgcg tcattgcacg ttctaccgca
     1921 tcgccatcac gtttttgcag ggcagagaaa atatcaagat gctggcgtaa cagcatttct
     1981 ggtggagaaa catggtcgaa actcatatag cgcacgcgat caacggtcgc tttcaggttc
     2041 tcaatggtat cccacgccag ttgacagtcg gcaatctgcg tcaggagttg atggaagttg
     2101 tcatcaagtt caaaaaaatc atccagttgc ttgcgctcaa tggcaatgcg ttgctggtga
     2161 agattttgtt ccagttggta gcactggctt tcggtaatca tgctcgccgc ccgacgcgcc
     2221 accgcgcact caatggcctg acggataaaa ctgccgtttc gcacctgggc catggaaatt
     2281 ttgttgacgt agctgccacg ctgcggacga atttgaatca ggccgttttc cgccagttta
     2341 ataaaggctt cacgaaccgg ctggcgtgac acattgaaac gaacagaaac ttctttttcc
     2401 gacaacggtg tgcctggtgc aatcaggcaa tggacaatgt cgcgacgaag aatacgataa
     2461 atctgctgat tgacaggctg tgtgggatta agttgcgttt cgacggtcat tcgttcttgc
     2521 ttgtgagtga gttaactgca tgagtctacc acttttttgt gacaagcaat aacccggcag
     2581 ttacgccggg tataaaaatt actgacggtg gacattcagt ccggcatagc tttgggtaac
     2641 cggcatcatt tccagggtat tgatattgac gtgagcaggc agcgttgaca cccaccagac
     2701 ggcttcgctg acatcttctg gcgtcaatgc aacggtattt tgataggttt tttccgcttt
     2761 accgtcatcg cctttaaagc ggacattgga aaactcggtg ccacccacca gacccggttc
     2821 gatgtcggtg acgcgcaccg ccgtaccatg caggtccgta cgcagattca ggctaaactg
     2881 acgaacaaac gctttcgtcg caccgtaaac gttaccaccg gcataaggcc agctacctgc
     2941 cgttgagcca atgttaataa tatgaccatg attacgttca accatacccg gtaagacggc
     3001 gcgcgtcata tataccaggc ctttgttgtt ggtatcaatc atcgtttccc agtcttcaac
     3061 gctggcttta tgcgcaggct ccatgcccaa cgccaggccg gcattattta ccaggatatc
     3121 aatattgcac cactcggcag ggagcgatgc cagcatctct tcaatagcgg cgcggttgcg
     3181 aacgtccagt tgggcgatat acagattatc tcccagttcg tcttttaact cctgcaaccg
     3241 ctcctggcga cggccagtgg cgataacttt atgcccttgt tgaataaaac gacgagtaat
     3301 gcattcacca aaacctgccg ttgctccagt tactaaaacg atcatctcac tgttcctcaa
     3361 cgcttttgtg tgtcaataac atagcacgtg atgcttaaga gcgggtaata ctggtttgca
     3421 agattgcact ccgttatcag taagcctacg ctatggaaca cctcgcgttc aggagaagaa
     3481 aatgacaaca atgaatcctt tccttgtgca aagcacactg ccgtatctgg ctcctcattt
     3541 tgatcaaatt gccaatcatc actatcgccc ggcattcgat gagggaatgc agcaaaagcg
     3601 ggcagaaatt gctgccatcg cgcttaaccc gcaaacgcct gatttcaaca atactattct
     3661 ggcactggaa caaagcggag aattacttac ccgcgttacc agcgtctttt ttgcgatgac
     3721 tgcggcgcat accaatgatg aattacagcg tcttgatgaa cagttttccg ctgaactggc
     3781 ggaactggct aatgatatct atctgaacgg tgaattattc gcgcgggtag atgctgtctg
     3841 gcagcgccgt gaatccctgg ggcttgatag tgaatccatc cgcctggtgg aggtgattca
     3901 tcaacgtttt gtccttgccg gagccaaact tgcgcaagct gataaagcaa aattaaaagt
     3961 actgaataca gaagctgcga cactgaccag tcagtttaac cagcggttac tggcagcaaa
     4021 taaatccggc ggtctggttg tgaacgatat cgcgcagctg gcaggaatga gtgagcaaga
     4081 gattgcgctg gcggcagagg cggctcgcga gaaaggtctg gataacaaat ggctgattcc
     4141 gctgctgaat accacccaac aaccggcgct tgccgaaatg cgcgatcgtg cgacgcgtga
     4201 aaaactgttt attgcgggct ggacgcgagc ggaaaaaaat gatggcaatg atacccgcgc
     4261 tatcattcaa cgtctggtgg agatccgtgc acaacaggcg acattacttg gttttcctca
     4321 ttatgccgca tggaaaatcg ccgatcagat ggcaaaaaca cctgaagcag cgctcaactt
     4381 tatgcgggaa attgttccag cggcgcgtca acgtgcgagc gatgaattag cctccataca
     4441 ggcggttatt gataagcagc agggcgggtt tagcgcgcag ccgtgggact gggcatttta
     4501 tgccgaacag gtacggcggg agaaatttga tcttgatgag gcgcagctca agccatattt
     4561 tgaattaaac acggtgttga atgaaggtgt attctggacc gcgaatcagc tcttcggtat
     4621 taagtttgtc gaacgttttg atattcctgt ctaccatcct gacgttcgtg tgtgggaaat
     4681 ttttgatcat aatggcgtgg gactggcgtt attttacggt gatttcttcg cccgtgattc
     4741 aaaaagcggc ggtgcatgga tgggcaattt tgttgagcaa tcaacgctta atgaaacgca
     4801 ttcggtaatt tataacgttt gcaattatca gaaacccgct gccggtgagc ctgcgttgtt
     4861 actctgggat gatgtcataa ccttattcca tgaatttggt catacgctgc acggcctttt
     4921 tgcccgccag cgttatgcca cgctttccgg caccaacacg ccgcgtgatt ttgtcgaatt
     4981 tccgtcgcaa atcaacgaac actgggcaac gcatccgcag gtattcgctc gctacgcccg
     5041 gcattatcag agcggggcag caatgcctga cgaactgcaa cagaaaatgc gtaatgccag
     5101 cctgttcaac aaagggtatg agatgagcga actgcttagc gccgcacttc tcgatatgcg
     5161 ctggcattgc ctggaagaaa acgaagcaat gcaggatgtc gatgattttg aattgcgggc
     5221 gctggtggcg gaaaatatgg atcttcctgc tataccgcca cgctatcgca gcagttattt
     5281 cgcccatatt tttggtggcg gatatgccgc aggttattac gcttatctgt ggacacaaat
     5341 gttggccgat gatggttatc agtggtttgt tgagcagggc ggattaacgc gtgaaaatgg
     5401 gctgcgtttt cgcgaggcga tcctttccag aggtaacagc gaagatctgg aacgcctgta
     5461 tcgacaatgg cgcggtaagg ctcctcagat tatgccgatg ctgcaacatc gtggcttgaa
     5521 catataaaca ccaccaatgg ctgtcctgga gatatcgcca ggccagcctg gttatagcag
     5581 taattgcagc agctgggcga gggcaaacct caccgctaaa gctaataccg tttcgcagtc
     5641 gccagcaaaa tgcataaccg cagtataggt ctggccttta atatgccacg cgaaccagac
     5701 ggtacccgct ggcgtaccat cttcaccgcc ctccggtccg ccgtagccgg taatggcaat
     5761 acttacatca gcatccgcac gctctatggc tccggttgcc atttctgctg ccactttctc
     5821 actcaccgca gaatatcgtt caagagattg ctggcttacg ctgaggattt tcatctttgc
     5881 ctgatcggtg aaagtaacaa agcctgcacc gtaaaatttg ggtgtatctt cagctgcaca
     5941 cagggcgcta gccaacttac cgccggtgca tgattcggcg gtagttaagc gtaattgatc
     6001 cgcgataagt ctttgactca acgcggatgt caggttttca atagtgtcgg tatctgcgag
     6061 ttgtacaatt ttatctttat taatgttcac tgttttttgt
//
LOCUS       DAZKHF020000257         6035 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2201S31241
            SAMN43267800-rid25106413.denovo.259, whole genome shotgun sequence.
ACCESSION   DAZKHF020000257 DAZKHF020000000
VERSION     DAZKHF020000257.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267800
            Sequence Read Archive: SRR30307263
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6035)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6035)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 6035)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350581.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350581.2
            Genome Coverage       :: 151x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:21:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,652
            CDSs (total)                      :: 5,557
            Genes (coding)                    :: 5,320
            CDSs (with protein)               :: 5,320
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 237
            CDSs (without protein)            :: 237
            Pseudo Genes (ambiguous residues) :: 0 of 237
            Pseudo Genes (frameshifted)       :: 102 of 237
            Pseudo Genes (incomplete)         :: 130 of 237
            Pseudo Genes (internal stop)      :: 52 of 237
            Pseudo Genes (multiple problems)  :: 41 of 237
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6035
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267800-rid25106413.denovo.259"
                     /serotype="O 26:H11"
                     /isolate="2201S31241"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-01"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            238..519
                     /locus_tag="ACHOSC_RS20285"
                     /old_locus_tag="pgaptmp_004057"
     CDS             238..519
                     /locus_tag="ACHOSC_RS20285"
                     /old_locus_tag="pgaptmp_004057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313069.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjcB family protein"
                     /protein_id="HGU4549886.1"
                     /translation="MATLTTGVVLLRWQLLSAVMMFLASTLNIRFRRSDYVGLAVISS
                     GLGVVSACWFAMGLLGITMADITAIWHNIELVMIEEMNQTPPQWPMILT"
     gene            complement(618..1154)
                     /gene="ssb1"
                     /locus_tag="ACHOSC_RS20290"
                     /old_locus_tag="pgaptmp_004058"
     CDS             complement(618..1154)
                     /gene="ssb1"
                     /locus_tag="ACHOSC_RS20290"
                     /old_locus_tag="pgaptmp_004058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein SSB1"
                     /protein_id="HGU4549887.1"
                     /translation="MASRGVNKVILVGNLGQDPEVRYMPNGGAVANITLATSESWRDK
                     ATGEMKEQTEWHRVVLFGKLAEVASEYLRKGSQVYIEGQLRTRKWTDQSGQDRYTTEV
                     VVNVGGTMQMLGGRQGGGAPAGGNIGGGQPQGGWGQPQQPQGGNQFSGGAQSRPQQSA
                     PAAPSNEPPMDFDDDIPF"
     gene            1408..4230
                     /gene="uvrA"
                     /locus_tag="ACHOSC_RS20295"
                     /old_locus_tag="pgaptmp_004059"
     CDS             1408..4230
                     /gene="uvrA"
                     /locus_tag="ACHOSC_RS20295"
                     /old_locus_tag="pgaptmp_004059"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HGU4549888.1"
                     /translation="MDKIEVRGARTHNLKNINLVIPRDKLIVVTGLSGSGKSSLAFDT
                     LYAEGQRRYVESLSAYARQFLSLMEKPDVDHIEGLSPAISIEQKSTSHNPRSTVGTIT
                     EIHDYLRLLFARVGEPRCPDHDVPLAAQTVSQMVDNVLSQPEGKRLMLLAPIIKERKG
                     EHTKTLENLASQGYIRARIDGEVCDLSDPPKLELQKKHTIEVVVDRFKVRDDLTQRLA
                     ESFETALELSGGTAVVADMDDPKAEELLFSANFACPICGYSMRELEPRLFSFNNPAGA
                     CPTCDGLGVQQYFDPDRVIQNPELSLAGGAIRGWDRRNFYYFQMLKSLADHYKFDVEA
                     PWGSLSANVHKVVLYGSGKENIEFKYMNDRGDTSIRRHPFEGVLHNMERRYKETESSA
                     VREELAKFISNRPCASCEGTRLRREARHVYVENTPLPAISDMSIGHAMEFFNNLKLAG
                     QRAKIAEKILKEIGDRLKFLVNVGLNYLTLSRSAETLSGGEAQRIRLASQIGAGLVGV
                     MYVLDEPSIGLHQRDNERLLGTLIHLRDLGNTVIVVEHDEDAIRAADHVIDIGPGAGV
                     HGGEVVAEGPLEAIMAVPESLTGQYMSGKRKIEVPKKRVPANPEKVLKLTGARGNNLK
                     DVTLTLPVGLFTCITGVSGSGKSTLINDTLFPIAQRQLNGATIAEPAPYRDIQGLEHF
                     DKVIDIDQSPIGRTPRSNPATYTGVFTPVRELFAGVPESRARGYTPGRFSFNVRGGRC
                     EACQGDGVIKVEMHFLPDIYVPCDQCKGKRYNRETLEIKYKGKTIHEVLDMTIEEARE
                     FFDAVPALARKLQTLMDVGLTYIRLGQSATTLSGGEAQRVKLARELSKRGTGQTLYIL
                     DEPTTGLHFADIQQLLDVLHKLRDQGNTIVVIEHNLDVIKTADWIVDLGPEGGSGGGE
                     ILVSGTPETVAECEASHTARFLKPML"
     gene            complement(4265..4621)
                     /locus_tag="ACHOSC_RS20300"
                     /old_locus_tag="pgaptmp_004060"
     CDS             complement(4265..4621)
                     /locus_tag="ACHOSC_RS20300"
                     /old_locus_tag="pgaptmp_004060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MmcQ/YjbR family DNA-binding protein"
                     /protein_id="HGU4549889.1"
                     /translation="MTISELLQYCMAKPGAEQSVHNDWKATQIKVEDVLFAMVKEVEN
                     RPAVSLKTSPELAELLRQQHSDVRPSRHLNKAHWSTVYLDGSLPDSQIYYLVDASYQQ
                     AVNLLPEEKRKLLVQL"
     gene            complement(4625..5041)
                     /locus_tag="ACHOSC_RS20305"
                     /old_locus_tag="pgaptmp_004061"
     CDS             complement(4625..5041)
                     /locus_tag="ACHOSC_RS20305"
                     /old_locus_tag="pgaptmp_004061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="secondary thiamine-phosphate synthase enzyme
                     YjbQ"
                     /protein_id="HGU4549890.1"
                     /translation="MWYQKTLTLSAKSRGFHLVTDEILNQLADMPRVNIGLLHLLLQH
                     TSASLTLNENCDPTVRHDMERFFLRTVPDNGNYEHDYEGADDMPSHIKSSMLGTSLVL
                     PVHKGRIQTGTWQGIWLGEHRIHGGSRRIIATLQGE"
     gene            complement(5152..5865)
                     /gene="aphA"
                     /locus_tag="ACHOSC_RS20310"
                     /old_locus_tag="pgaptmp_004062"
     CDS             complement(5152..5865)
                     /gene="aphA"
                     /locus_tag="ACHOSC_RS20310"
                     /old_locus_tag="pgaptmp_004062"
                     /EC_number="3.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_function: GO:0003993 - acid phosphatase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acid phosphatase AphA"
                     /protein_id="HGU4549891.1"
                     /translation="MRKITQAISAVCLLFALNSSAVALASSPSPLNPGTNVARLAEQA
                     PIHWVSVAQIENSLAGRPPMAVGFDIDDTVLFSSPGFWRGKKTFSPESEDYLKNPVFW
                     EKMNNGWDEFSIPKEVARQLIDMHVRRGDAIFFVTGRSPTKTETVSKTLADNFHIPAT
                     NMNPVIFAGDKLGQNTKSQWLQDKNIRIFYGDSDNDITAARDVGARGIRILRASNSTY
                     KPLPQAGAFGEEVIVNSEY"
BASE COUNT         1420 a         1587 c         1581 g         1447 t
ORIGIN      
        1 gcgggaagat atgcccttaa tgccactgaa aaaccacata atctgtacag caataaaagt
       61 cacggcctaa tatggctaac aaacaatcaa ttgtgatata gttcacaaaa ttaatgaaac
      121 aaacagagtg tttcattttt gtgttctgca agccccgcga tagccagagt gtaccacgcc
      181 tcccgtgaac aacgccgcgc tgtccagggc atcggctctt ttcagaggaa ttgtttgatg
      241 gctaccctca ccactggcgt ggttcttctt cgctggcaac ttcttagtgc cgtaatgatg
      301 tttctggcca gcacactcaa catccgtttt cgtcggtctg attatgtcgg gcttgcggtg
      361 atcagcagcg gtctgggcgt tgtttccgcc tgttggttcg caatggggtt gcttggcatc
      421 acaatggcgg acatcaccgc tatctggcac aacatcgagt tggtgatgat agaagagatg
      481 aatcagacac cgccacaatg gccaatgatt ttgacctgat acgtagaagc ctccataacg
      541 gaggcttctt ttttatggct ggcagatgct ttaatcatcc accttaaaac aatataacct
      601 attgttttaa tgacaaatca gaacggaatg tcatcatcaa agtccatcgg cggctcgtta
      661 gacggcgctg ccggagcgga ctgctgcggg cgagactgcg cgccgccgct gaactgattg
      721 ccaccctgcg gctgctgtgg ctgaccccaa ccgccctgcg gctgaccacc accgatattg
      781 ccacctgccg gagcgccacc accctgacga ccacccagca tctgcatggt gccgccaacg
      841 ttcaccacga cttctgtggt gtagcgatcc tgaccggatt gatcggtcca tttacgggta
      901 cgcagctgac cttcgatata aacctgagaa cctttacgca gatattcgct cgccacttct
      961 gccagtttgc cgaacagcac aacgcggtgc cattcagtct gttctttcat ctcgccggtc
     1021 gctttatcac gccaggattc ggaagtagcc agcgtaatgt tggcaactgc gccaccattt
     1081 ggcatgtagc gtacttccgg gtcctgaccc agattaccaa cgagaataac cttgtttacg
     1141 cctctgctgg ccatgttcgt gtctcctgaa aaaaatcgtt ctgaataagt gtaaacgcgc
     1201 gattgtacca ttaccaatag cgcttttact atgttgtgac ctcggttccg ggaaacaaac
     1261 ctggtcagac attgttacac aacactccgg gtaatgcatt ccaatactgt atattcattc
     1321 aggtcaattt gtgtcataat taaccgtttg tgatcgccgg tagcaccatg ccaccgggca
     1381 aaaaagcgtt taatccggga aaggtgaatg gataagatcg aagttcgggg cgcccgcacc
     1441 cataatctca aaaacatcaa cctcgttatc ccccgcgaca agctcattgt cgtgaccggg
     1501 ctttcgggtt ctggcaaatc ctcgctcgct ttcgacacct tatatgccga agggcagcgc
     1561 cgttacgttg aatccctttc cgcctacgcg cggcagtttc tgtcactgat ggaaaagccg
     1621 gacgtcgatc atattgaggg gctttctcct gccatctcaa ttgagcagaa atcgacgtct
     1681 cataacccgc gttctacggt ggggacaatc accgaaatcc acgactattt gcgtttgttg
     1741 ttcgcccgcg tcggcgaacc gcgctgtccg gaccacgacg tcccgctggc ggcgcaaacc
     1801 gtcagccaga tggtggataa cgtgctgtcg cagccggaag gcaagcgtct gatgctgctc
     1861 gcgccaatca ttaaagagcg caaaggcgaa cacaccaaaa cgctggagaa cctggcaagc
     1921 cagggttaca tccgtgctcg tattgatggc gaagtctgcg atctttccga tccgccgaaa
     1981 ctggaactgc aaaagaaaca taccattgaa gtggtggttg atcgcttcaa ggtgcgtgac
     2041 gatcttaccc aacgtcttgc ggagtcgttt gaaaccgcgc tggagctttc cggtggtaca
     2101 gcggtagtgg cggatatgga cgacccgaaa gcggaagagc tgctgttctc cgccaacttc
     2161 gcctgcccaa tttgcggcta cagtatgcgt gaactggagc cgcgactgtt ttcgtttaac
     2221 aacccggcag gtgcctgccc gacctgtgac ggccttggcg tacagcaata tttcgatcct
     2281 gaccgcgtga tccaaaaccc cgagctgtca ctggctggcg gtgcgatccg tggctgggat
     2341 cgccgcaact tctattactt ccagatgctg aaatcgctgg cagatcacta taagttcgac
     2401 gtcgaagcgc cgtggggcag cctgagcgcg aacgtgcata aagtggtgtt gtacggttct
     2461 ggcaaagaaa acattgaatt caaatacatg aacgatcgtg gcgatacctc catccgtcgt
     2521 catccgttcg aaggcgtgct gcacaatatg gagcgccgtt ataaagagac agaatccagt
     2581 gcggtacgtg aagaattagc caagtttatc agcaatcgcc catgcgccag ctgcgaaggg
     2641 acgcgtctgc gtcgggaagc gcgccacgtt tatgtcgaga atacgccgct gcctgctatc
     2701 tccgacatga gcatcggtca tgcgatggaa ttcttcaaca atctcaaact cgctggtcaa
     2761 cgggcgaaga ttgcggaaaa aatccttaaa gagatcggcg atcgtttgaa attcctcgtt
     2821 aacgtcggcc tgaattacct gacgctttcc cgctcggcag aaacgctttc cggcggtgaa
     2881 gcccagcgta tccgtctggc gagccagatt ggtgcgggcc tggttggcgt tatgtacgtg
     2941 ctggacgagc catctatcgg cctgcaccag cgcgataacg agcgcctgtt gggtacgctt
     3001 atccatctgc gcgatctcgg taataccgtg attgtggtgg agcacgacga agacgcaatt
     3061 cgcgccgctg accatgtgat cgacattggc ccgggcgcag gtgttcacgg cggtgaagtg
     3121 gtcgcagaag gtccgctgga agcgattatg gcggtgccgg agtcgttgac cgggcagtac
     3181 atgagcggca aacgcaagat tgaagtgccg aagaaacgcg ttccggcgaa tccggaaaaa
     3241 gtgctgaagc tgacaggcgc acgcggcaac aacctgaagg acgtgacgct gacgctgccg
     3301 gtgggtctgt ttacctgcat caccggggtt tcaggttccg gtaaatcgac gctgattaac
     3361 gacacactgt tcccgattgc ccaacgccag ttgaatgggg cgaccatcgc cgaaccagca
     3421 ccgtatcgcg atattcaggg gctggagcat ttcgataaag tgatcgatat cgaccaaagc
     3481 ccaattggtc gtactccacg ttctaacccg gcgacctata ccggcgtgtt tacgcctgtg
     3541 cgcgaactgt ttgcgggcgt accggaatcc cgtgcgcgtg gttatacgcc aggacgtttc
     3601 agctttaacg tccgtggcgg gcgctgcgaa gcctgtcagg gcgacggtgt gatcaaagta
     3661 gagatgcact tcctgccgga tatctacgtg ccgtgcgatc agtgtaaagg taaacgctat
     3721 aaccgtgaaa cgctggaaat taagtacaaa ggcaaaacca tccacgaagt gctggatatg
     3781 accatcgaag aggcgcgtga gttctttgat gccgtacctg cactggcgcg taagctgcaa
     3841 acgttgatgg acgttggcct gacgtacatt cgcctggggc agtccgcaac cacactttct
     3901 ggtggtgaag cccagcgcgt gaagctggcg cgtgagctgt caaaacgcgg caccgggcag
     3961 acgctgtata ttctcgacga gccgaccacc ggtctgcact tcgccgatat tcagcaactg
     4021 ctcgacgtac tgcataaact gcgcgatcag ggcaacacca ttgtggtgat tgagcacaat
     4081 ctcgacgtga tcaaaaccgc tgactggatt gtcgacctgg gaccagaagg cggcagtggc
     4141 ggcggcgaaa tcctcgtctc cggtacgcca gaaaccgtcg cggagtgcga agcttcgcat
     4201 acggcacgct tcctcaagcc gatgctgtaa tcgttaaggc cgctttctga gcggcctttt
     4261 cctttcagag ttgtaccagc aatttacgct tttcttccgg tagtaaattc actgcctgct
     4321 gataagacgc atccaccaga taatagattt gcgaatccgg cagcgaaccg tcgagataca
     4381 cggtgctcca gtgcgcttta ttcagatggc ggctaggacg cacatcgctg tgctgctgac
     4441 gtagcagctc cgccagctcc gggctggttt tcagcgaaac agctgggcga ttttctactt
     4501 ctttcaccat cgcaaacagt acatcttcca ctttgatctg cgtcgctttc cagtcgttat
     4561 gcacgctctg ttctgcgcct ggttttgcca tgcaatattg tagcaactcc gaaatggtca
     4621 ttttttactc cccttgtagt gtcgcgatga tgcgacgcga tccgccgtgg atgcgatgtt
     4681 cccccagcca gatgccttgc caggtgccgg tctgaatacg ccctttatgt accggcaata
     4741 caagcgatgt tcccagcatt gaggatttga tatgagaagg catatcgtct gctccctcat
     4801 agtcatgctc ataatttccg ttgtcgggaa cggtgcggag gaaaaaacgc tccatgtcgt
     4861 ggcgtacggt gggatcgcag ttctcattaa gtgtcagaga ggcggaggta tgttgcagca
     4921 acagatgcag taagccgatg ttaacgcgcg gcatatcagc cagctgattc agaatttcat
     4981 ccgttaccag atgaaaccca cgagatttgg cgctaagcgt gagcgtcttt tgataccaca
     5041 tgtgctgctc cttgataaaa ctctcttaat cagtttgcag caagacagcg aaaggataaa
     5101 ggtgtgatta aaaaaacagc attcgggaga gcgtcacgct ctcccgctct gtcagtattc
     5161 tgaattgacg atcacctctt caccaaacgc acccgcttgt ggcaagggtt tgtaggtaga
     5221 gttggaggcg cgcagaatgc ggataccacg agcgccgaca tcgcgtgcgg cggtaatatc
     5281 attatcagaa tcgccataaa aaattcggat atttttatcc tgcagccatt gcgattttgt
     5341 attttgccct agtttatcgc ccgcaaagat caccggattc atgttggtgg caggaatatg
     5401 aaaattatcc gccagcgttt ttgaaaccgt ttctgttttc gtcggactac gaccagtcac
     5461 aaagaagatc gcgtcaccgc ggcgtacatg catatcaatc agctggcgag cgacctcttt
     5521 tggaatgctg aattcatccc agccattgtt cattttttcc cagaacacag gatttttcag
     5581 ataatcttcg ctttctggcg agaaggtttt tttgccgcgc cagaagcccg gactggaaaa
     5641 aagtaccgtg tcatcgatat caaaccccac cgccattggc ggacgccctg cgaggctatt
     5701 ttcaatttgt gcgaccgaaa cccaatgaat gggtgcctgt tcagcaagcc tggcaacgtt
     5761 agtcccaggg ttaagcggtg aaggagatga ggccagggca acagcggaac tgtttagagc
     5821 gaacaataag caaacggcac tgattgcctg tgtgatcttg cgcatatttt tccctaaata
     5881 gtcagtatgt tgaaactttt tattgtgaga tttgttgcaa aaaccatctg accataacgc
     5941 cagctgccgg ggaaaggaag gattatctgc ggtttttgtg atgaaaagca ggatgattat
     6001 atttaaccca gtaaatacgt tcctgtcttt ttttg
//
LOCUS       DAZKHF020000258         6018 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2201S31241
            SAMN43267800-rid25106413.denovo.260, whole genome shotgun sequence.
ACCESSION   DAZKHF020000258 DAZKHF020000000
VERSION     DAZKHF020000258.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267800
            Sequence Read Archive: SRR30307263
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6018)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6018)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 6018)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350581.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350581.2
            Genome Coverage       :: 151x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:21:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,652
            CDSs (total)                      :: 5,557
            Genes (coding)                    :: 5,320
            CDSs (with protein)               :: 5,320
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 237
            CDSs (without protein)            :: 237
            Pseudo Genes (ambiguous residues) :: 0 of 237
            Pseudo Genes (frameshifted)       :: 102 of 237
            Pseudo Genes (incomplete)         :: 130 of 237
            Pseudo Genes (internal stop)      :: 52 of 237
            Pseudo Genes (multiple problems)  :: 41 of 237
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6018
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267800-rid25106413.denovo.260"
                     /serotype="O 26:H11"
                     /isolate="2201S31241"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-01"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            202..1293
                     /gene="ompC"
                     /locus_tag="ACHOSC_RS20315"
                     /old_locus_tag="pgaptmp_004063"
     CDS             202..1293
                     /gene="ompC"
                     /locus_tag="ACHOSC_RS20315"
                     /old_locus_tag="pgaptmp_004063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porin OmpC"
                     /protein_id="HGU4549892.1"
                     /translation="MKVKVLSLLVPALLVAGAANAAEVYNKDGNKLDLYGKVDGLHYF
                     SDNKDVDGDQTYMRLGFKGETQVTDQLTGYGQWEYQIQGNSAENENNSWTRVAFAGLK
                     FQDVGSFDYGRNYGVVYDVTSWTDVLPEFGGDTYGSDNFMQQRGNGFATYRNTDFFGL
                     VDGLNFAVQYQGKNGSVSGEGMTNNGREALRQNGDGVGGSITYDYEGFGIGAAVSSSK
                     RTDDQNSPLYIGNGDRAETYTGGLKYDANNIYLAAQYTQTYNATRVGSLGWANKAQNF
                     EAVAQYQFDFGLRPSLAYLQSKGKNLGRGYDDEDILKYVDVGATYYFNKNMSTYVDYK
                     INLLDDNQFTRDAGINTDNIVALGLVYQF"
     gene            1405..2460
                     /gene="apbE"
                     /locus_tag="ACHOSC_RS20320"
                     /old_locus_tag="pgaptmp_004064"
     CDS             1405..2460
                     /gene="apbE"
                     /locus_tag="ACHOSC_RS20320"
                     /old_locus_tag="pgaptmp_004064"
                     /EC_number="2.7.1.180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0017013 - protein flavinylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD:protein FMN transferase ApbE"
                     /protein_id="HGU4549893.1"
                     /translation="MEISFTRVALLAAALFFVGCDQKPQTAKTHATEVTVLEGKTMGT
                     FWRASIPGIDAKRSAELKEKIQTQLDADDQLLSTYKKDSALMRFNDSQSLSPWPVSEA
                     MADIVTTSLRIGARTDGAMDITVGPLVNLWGFGPEQQPVQIPSQEQIDAMKAKTGLQH
                     LTVINQSHQQYLQKDLPDLYVDLSTVGEGYAADHLARLMEQEGISRYLVSVGGALNSR
                     GMNGEGLPWRVAIQKPTDKENAVQAVVDINGHGISTSGSYRNYYELDGKRLSHVIDPQ
                     TGRPIEHNLVSVTVIAPTALEADAWDTGLMVLGPEKAKEVVRREGLAVYMITKEGDSF
                     KTWMSPQFKSFLVSEKN"
     gene            2534..3598
                     /gene="ada"
                     /locus_tag="ACHOSC_RS20325"
                     /old_locus_tag="pgaptmp_004065"
     CDS             2534..3598
                     /gene="ada"
                     /locus_tag="ACHOSC_RS20325"
                     /old_locus_tag="pgaptmp_004065"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional DNA-binding transcriptional
                     regulator/O6-methylguanine-DNA methyltransferase Ada"
                     /protein_id="HGU4549894.1"
                     /translation="MKNATCLTDDQRWQSVLARDPNADSEFVFAVRTTGIFCRPSCRA
                     RHALRENVSFYANACEALAAGFRPCKRCQPDKANAQQHRLDKITHACRLLEQETPVTL
                     EALADQVAMSPFHLHRLFKATTGMTPKAWQQAWRARRLRESLAKGESVTTSILNAGFP
                     DSSSYYRKADETLGMTAKQFRHGGENLAVRYALADCELGRCLVAESERGICAILLGDD
                     DATLISELQQMFPAADNAPADLMFQQHVREVIASLNQRDTPLTLPLDIRGTAFQQQVW
                     QALRTIPCGETVSYQQLANAIGKPKAVRAVASACAANKLAIIIPCHRVVRGDGTLSGY
                     RWGVSRKAQLLRREAENEER"
     gene            3598..4248
                     /gene="alkB"
                     /locus_tag="ACHOSC_RS20330"
                     /old_locus_tag="pgaptmp_004066"
     CDS             3598..4248
                     /gene="alkB"
                     /locus_tag="ACHOSC_RS20330"
                     /old_locus_tag="pgaptmp_004066"
                     /EC_number="1.14.11.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA oxidative demethylase AlkB"
                     /protein_id="HGU4549895.1"
                     /translation="MLDLFADAEPWQEPLAAGAVILHRFAFNAAEQLIRDINDVASQS
                     PFRQMVTPGGYTMSVAMTNCGHLGWTTHRQGYLYSPIDPQTNKPWPAMPQSFHDLCQR
                     AATAAGYPDFQPDACLINRYAPGAKLSLHQDKDEPDLRAPIVSVSLGLPAIFQFGGLK
                     RNDPLKRLLLEHGDVVVWGGESRLFYHGIQPLKAGFHPLTGDCRYNLTFRQAGKKE"
     gene            4324..5967
                     /gene="yojI"
                     /locus_tag="ACHOSC_RS20335"
                     /old_locus_tag="pgaptmp_004067"
     CDS             4324..5967
                     /gene="yojI"
                     /locus_tag="ACHOSC_RS20335"
                     /old_locus_tag="pgaptmp_004067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="microcin J25 efflux ABC transporter YojI"
                     /protein_id="HGU4549896.1"
                     /translation="MELLVLVWRQYRWPFISVMALSLASAALGIGLIAFINQRLIETA
                     DTSLLVLPEFLGLLLLLMAVTLGSQLALTTLGHHFVYRLRSEFIKRILDTNVERIEQL
                     GSASLLAGLTSDVRNITIAFVRLPELVQGIILTIGSAAYLWMLSGKMLLVTAIWMAIT
                     IWGGFVLVARVYKHMATLRETEDRLYTDFQTVLEGRKELTLNRERAEYVFNNLYIPDA
                     QEYRHHIIRADTFHLSAVNWSNIMMLGAIGLVFWMANSLGWADTNVAATYSLTLLFLR
                     TPLLSAVGALPTLLTAQVAFNKLNKFALAPFKAEFPRPQAFPNWQTLELRNVTFAYQD
                     NAFSVGPINLTIKRGELLFLIGGNGSGKSTLAMLLTGLYQPQSGEILLDGKPVSGEQP
                     EDYRKLFSAVFTDVWLFDQLLGPEGKPANPQLVEKWLAQLKMAHKLELSNGRIVNLKL
                     SKGQKKRVALLLALAEERDIILLDEWAADQDPHFRREFYQVLLPLMQEMGQTIFAISH
                     DDHYFIHADRLLEMRNGQLSELTGEERDAASRDAVARTA"
BASE COUNT         1432 a         1519 c         1633 g         1434 t
ORIGIN      
        1 aatcttaaaa agttcccttg catttacatt ttgaaacatc tatagcgata aatgatacat
       61 cttaaaagtt ttagtatcat attcgtgttg gattattctg catttttggg gagaatggac
      121 ttgccgactg attaatgagg gttaatcagt atgcagtggc ataaaaaagc aaataaaggc
      181 atataacaga gggttaataa catgaaagtt aaagtactgt ccctcctggt cccagctctg
      241 ctggtagcag gcgcagcaaa cgctgctgaa gtttacaaca aagacggcaa caaattagat
      301 ctgtacggta aagtagacgg cctgcactat ttctctgaca acaaagatgt agatggcgac
      361 cagacctaca tgcgtcttgg cttcaaaggt gaaactcagg ttactgacca gctgaccggt
      421 tacggccagt gggaatatca gatccagggc aacagcgctg aaaacgaaaa caactcctgg
      481 acccgtgtgg cattcgcagg tctgaaattc caggatgtag gttctttcga ctacggtcgt
      541 aactacggcg ttgtttacga cgtaacttcc tggaccgacg tactgccaga attcggtggc
      601 gacacttacg gttctgacaa cttcatgcag cagcgtggta acggtttcgc gacctaccgt
      661 aacactgact tcttcggtct ggttgacggc ctgaactttg ctgttcagta ccagggcaaa
      721 aacggtagcg taagcggcga aggcatgact aacaacggtc gtgaagcact gcgtcagaac
      781 ggcgacggcg ttggcggatc tatcacttat gattacgaag gcttcggtat cggtgctgca
      841 gtttccagct ccaaacgtac tgatgatcag aacagcccgc tgtacatcgg taacggcgac
      901 cgtgctgaaa cctacactgg tggtctgaaa tacgacgcta acaacatcta cctggctgct
      961 cagtacaccc agacctacaa cgcaactcgc gtaggttccc tgggttgggc gaacaaagca
     1021 cagaacttcg aagctgttgc tcagtaccag ttcgactttg gtctgcgtcc gtccctggct
     1081 tacctgcagt ctaaaggtaa aaacctgggt cgtggctacg acgacgaaga tatcctgaaa
     1141 tatgttgatg ttggtgcgac ctactacttc aacaaaaaca tgtccaccta tgttgactac
     1201 aaaatcaacc tgctggacga caaccagttc actcgtgacg ctggcatcaa cactgataac
     1261 atcgtagctc tgggtctggt ttaccagttc taatctcgat tgatatcgaa caaagggcct
     1321 gcgggccctt ttttcattgt tttcagcgta caaacacagt tttttggtgt actcttgcga
     1381 ccgttcgcat gaggataatc acgtatggaa ataagcttta cccgcgtggc actgctggct
     1441 gccgcgctct tctttgttgg ttgcgatcaa aaaccacaaa ccgccaaaac ccacgctact
     1501 gaagttaccg ttcttgaagg caaaactatg ggtaccttct ggcgtgccag catcccgggc
     1561 attgacgcca aacgcagtgc cgaacttaaa gaaaagattc agacccagct ggacgccgac
     1621 gatcagctgc tttcgaccta taaaaaagat tccgcgctga tgcgctttaa cgattcgcaa
     1681 agtttatcgc cgtggccggt aagtgaagcg atggccgata tcgtcaccac ctcgctgcgc
     1741 attggcgcaa ggaccgatgg tgcgatggat ataaccgtcg ggccgctggt gaatctgtgg
     1801 ggctttggcc cggaacaaca gccggttcaa attccgagcc aggaacagat cgatgcgatg
     1861 aaagccaaaa ccggcttaca gcacctgacg gtcattaatc agtcgcatca gcaatatctg
     1921 caaaaagacc taccggattt atatgtcgat ctctccaccg tcggtgaagg ttatgcggcg
     1981 gatcacctgg cacgcttgat ggagcaggaa gggatttccc gctatctggt gtcggtgggc
     2041 ggcgcgctga acagccgtgg tatgaacggt gaaggcctgc cgtggcgggt agcgattcaa
     2101 aaaccaaccg ataaagaaaa cgcggttcag gctgtggtgg atatcaacgg tcatggtatt
     2161 agcacctctg gcagttaccg taactattac gaactggacg gcaaacgtct ttctcatgtt
     2221 atcgatccgc aaaccgggcg tcccatcgaa cacaatctgg tatccgtgac ggtgattgct
     2281 ccgacggcgc tggaagccga tgcctgggat actggcttga tggtactcgg gccggagaaa
     2341 gccaaagagg ttgttcgccg ggaagggctg gcggtttata tgatcaccaa agaaggcgat
     2401 agctttaaaa cctggatgtc accacagttt aaaagcttcc ttgtcagcga aaaaaattaa
     2461 agcgcaagat tgctggtttt tgcgtgatgg tgaccgggca gactaaaggc tatccttaac
     2521 cagggagctg attatgaaaa acgccacatg cttaactgac gatcaacgct ggcaatctgt
     2581 cttagcccgc gacccgaatg ccgacagcga attcgttttc gccgtgcgca ccacaggcat
     2641 cttttgccgt ccgtcttgcc gcgccagaca tgccttgcgg gaaaatgtct ccttctacgc
     2701 aaatgcctgc gaggcactcg ccgccggatt tcgcccctgc aaacgctgcc aacctgataa
     2761 agccaatgcc cagcaacatc ggttggataa aatcacccac gcgtgtcgac tgctggaaca
     2821 ggaaacgcct gtaacgctgg aagccttagc cgaccaggtg gcgatgagtc catttcatct
     2881 acatcggttg tttaaagcga ctaccggaat gacgcctaaa gcctggcaac aggcctggcg
     2941 cgctcgccgt ttgcgcgaat cgctggcgaa aggggagagc gtgacgacgt ctattcttaa
     3001 cgccggattc cccgacagca gcagttacta tcgcaaagct gacgaaacgc tgggcatgac
     3061 ggctaaacaa ttccgtcacg gtggcgaaaa tctggcggtg cgttacgcgc tggctgattg
     3121 tgagctgggt cgttgcctgg tggcagaaag cgagcggggg atttgcgcga tattgctggg
     3181 cgatgatgac gcgacactaa tcagcgagtt gcagcagatg tttcccgctg ccgacaacgc
     3241 gcctgccgat ctgatgtttc agcaacatgt gcgtgaagtg atcgccagcc tcaatcaacg
     3301 cgatacgccg ctgacgttac cgctggacat tcgcggcact gcttttcagc aacaagtctg
     3361 gcaggcactg cgcacgatac cttgcggtga aaccgtcagt tatcagcaac tggctaacgc
     3421 catcggcaaa ccgaaagcgg tacgggccgt tgccagcgcc tgtgccgcca acaagctggc
     3481 tatcataata ccctgtcatc gggtggtccg tggtgatggc acactttccg gttaccgctg
     3541 gggcgtgtcg cgtaaagcgc aactgctgcg ccgcgaagct gaaaatgagg agaggtaatg
     3601 ttggatctgt ttgccgatgc tgaaccgtgg caagagccac tggcggctgg agcggtaatt
     3661 ttgcaccgtt ttgcttttaa cgctgcggag caactgatcc gcgatattaa tgacgttgcc
     3721 agccagtcgc cgtttcgcca gatggtcacc cccgggggat ataccatgtc ggtggcgatg
     3781 accaactgtg ggcatctggg ctggacgacc catcggcaag gttatcttta ttcgcccatt
     3841 gatccgcaaa caaataaacc gtggcccgcc atgccacaaa gctttcatga tttatgtcaa
     3901 cgtgcggcta cggcggcggg ctatccggat ttccagccag atgcttgtct tatcaaccgc
     3961 tacgctcctg gcgcgaaact gtcgctgcat caggataaag acgaaccgga tctgcgcgcg
     4021 ccgattgttt ctgtttctct tggcttaccc gcgatttttc aatttggtgg cctgaaacgt
     4081 aatgatccgc tcaaacgttt gttgttggaa catggcgatg tggtggtatg gggcggtgaa
     4141 tcgcggctgt tttatcacgg cattcaaccg ctgaaagcgg ggtttcatcc actcaccggc
     4201 gactgccgct acaacctgac attccgtcag gcaggtaaaa aagaataaaa ataagaatta
     4261 ttattgctgt gcgcgcgaag atgtttaaac tgcgggctgt aattcattgt ccgggttttc
     4321 tgcatggaac ttcttgtact tgtctggcgg cagtatcgct ggccatttat cagtgtgatg
     4381 gcgctaagcc tcgccagtgc ggcattaggc attggcttaa ttgcttttat caatcagcgc
     4441 cttatcgaaa cggcggatac cagtctgctg gtattgccgg agtttctggg attattgctg
     4501 ctgttgatgg cagtcactct cggatcgcaa ctggcgctca ccactttggg gcatcacttc
     4561 gtttaccgac tgcgtagtga atttatcaag cggattctgg ataccaacgt cgagcgcatt
     4621 gaacaactcg gtagcgcctc gttgctggcg ggattaacca gcgatgtgcg caatatcacc
     4681 attgcttttg tgcgtcttcc ggaactggtg caggggatca ttctcactat cggttccgcg
     4741 gcgtatctgt ggatgctgtc gggcaaaatg ttgctggtaa cggctatctg gatggcgatc
     4801 accatctggg gtggttttgt actggtggcg cgggtgtaca aacatatggc gaccctgcgt
     4861 gaaaccgaag acaggctgta tacggatttt caaactgtac tcgaagggcg caaagagctg
     4921 actctgaacc gggaacgcgc cgagtatgtg tttaacaacc tctacattcc tgatgcgcaa
     4981 gagtatcgcc accatattat ccgcgcagac accttccatc ttagcgccgt gaactggtca
     5041 aacatcatga tgttgggcgc aatcggcctg gtgttctgga tggcgaacag tctcggttgg
     5101 gctgatacca acgttgccgc gacctattcg ttgacgcttt tattcctgcg tacgccgctg
     5161 ctttcggcgg ttggcgcatt gccgacgctg ctgacggcgc aggtggcgtt taacaagctg
     5221 aacaaattcg cgctcgcgcc tttcaaagca gagtttccgc gcccgcaggc gtttcccaac
     5281 tggcaaacgc tggagctgcg taacgtgacg tttgcttatc aggataacgc gttttccgtt
     5341 ggtccgatta atctcaccat caaacgtggc gagctgctgt ttctgattgg cggcaacggt
     5401 agcggaaaat cgacgctggc gatgttgttg acgggcttgt atcagccaca aagcggcgaa
     5461 atcttgctgg atggcaaacc tgtcagcggc gaacaaccgg aagattatcg caaactgttt
     5521 tcggcagtgt ttaccgatgt ctggctgttt gatcaactgc tggggccgga gggtaaaccc
     5581 gctaacccgc aactggttga gaagtggctg gcgcagctga aaatggctca taagcttgag
     5641 ttaagcaacg ggcgtattgt taacctgaag ttatcaaaag ggcagaaaaa acgcgtggcg
     5701 ctgttgctgg cgctggcaga agaacgcgat attatcctgc tggatgaatg ggcggcggat
     5761 caggatccac acttccgtcg tgagttttat caggtgttgc tgccgctgat gcaggagatg
     5821 ggtcaaacta ttttcgccat cagtcatgat gatcattact ttatccacgc cgaccgcctg
     5881 ctggaaatgc gcaacgggca acttagcgag ctgacgggcg aagagcgcga tgccgcttcg
     5941 cgtgatgccg ttgcccggac ggcataatat tacgacgctg acctggcgga ctgctgccgt
     6001 caggtcaata tgctaaaa
//
LOCUS       DAZKHF020000259         5998 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2201S31241
            SAMN43267800-rid25106413.denovo.261, whole genome shotgun sequence.
ACCESSION   DAZKHF020000259 DAZKHF020000000
VERSION     DAZKHF020000259.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267800
            Sequence Read Archive: SRR30307263
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5998)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5998)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 5998)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350581.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350581.2
            Genome Coverage       :: 151x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:21:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,652
            CDSs (total)                      :: 5,557
            Genes (coding)                    :: 5,320
            CDSs (with protein)               :: 5,320
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 237
            CDSs (without protein)            :: 237
            Pseudo Genes (ambiguous residues) :: 0 of 237
            Pseudo Genes (frameshifted)       :: 102 of 237
            Pseudo Genes (incomplete)         :: 130 of 237
            Pseudo Genes (internal stop)      :: 52 of 237
            Pseudo Genes (multiple problems)  :: 41 of 237
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5998
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267800-rid25106413.denovo.261"
                     /serotype="O 26:H11"
                     /isolate="2201S31241"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-01"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            complement(26..178)
                     /gene="hokA"
                     /locus_tag="ACHOSC_RS20340"
                     /old_locus_tag="pgaptmp_004068"
     CDS             complement(26..178)
                     /gene="hokA"
                     /locus_tag="ACHOSC_RS20340"
                     /old_locus_tag="pgaptmp_004068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012540334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin HokA"
                     /protein_id="HGU4549897.1"
                     /translation="MPQKYRLLSLIVICFTLLFFTWMIRDSLCELHIKQGSYELAAFL
                     ACNLKE"
     gene            complement(503..2572)
                     /gene="glyS"
                     /locus_tag="ACHOSC_RS20345"
                     /old_locus_tag="pgaptmp_004069"
     CDS             complement(503..2572)
                     /gene="glyS"
                     /locus_tag="ACHOSC_RS20345"
                     /old_locus_tag="pgaptmp_004069"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001291772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004820 - glycine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006426 - glycyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit beta"
                     /protein_id="HGU4549898.1"
                     /translation="MSEKTFLVEIGTEELPPKALRSLAESFAANFTAELDNAGLAHGT
                     VQWFAAPRRLALKVANLAEAQPDREIEKRGPAIAQAFDAEGKPSKAAEGWARGCGITV
                     DQAERLTTDKGEWLLYRAHVKGESTEALLPNMVATSLAKLPIPKLMRWGASDVHFVRP
                     VHTVTLLLGDKVIPATILGIQSDRVIRGHRFMGEPEFTIDNADQYPEILRERGKVIAD
                     YEERKAKIKADAEEAARKIGGNADLSESLLEEVASLVEWPVVLTAKFEEKFLAVPAEA
                     LVYTMKGDQKYFPVYANDGKLLPNFIFVANIESKDPQQIISGNEKVVRPRLADAEFFF
                     NTDRKKRLEDNLPRLQTVLFQQQLGTLRDKTDRIQALAGWIAEQIGADVNHATRAGLL
                     SKCDLMTNMVFEFTDTQGVMGMHYARHDGEAEDVAVALNEQYQPRFAGDDLPSNPVAC
                     ALAIADKMDTLAGIFGIGQHPKGDKDPFALRRAALGVLRIIVEKNLNLDLQTLTEEAV
                     RLYGDKLTNANVVDDVIDFMLGRFRAWYQDEGYTVDTIQAVLARRPTRPADFDARMKA
                     VSHFRTLEAAAALAAANKRVSNILAKSDEVLSDRVNASTLKEPEEIKLAMQVVVLRDK
                     LEPYFAEGRYQDALVELAELREPVDAFFDKVMVMVDDKELRLNRLTMLEKLRELFLRV
                     ADISLLQ"
     gene            complement(2582..3493)
                     /gene="glyQ"
                     /locus_tag="ACHOSC_RS20350"
                     /old_locus_tag="pgaptmp_004070"
     CDS             complement(2582..3493)
                     /gene="glyQ"
                     /locus_tag="ACHOSC_RS20350"
                     /old_locus_tag="pgaptmp_004070"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004820 - glycine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006426 - glycyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit alpha"
                     /protein_id="HGU4549899.1"
                     /translation="MQKFDTRTFQGLILTLQDYWARQGCTIVQPLDMEVGAGTSHPMT
                     CLRALGPEPMAAAYVQPSRRPTDGRYGENPNRLQHYYQFQVVIKPSPDNIQELYLGSL
                     KELGMDPTIHDIRFVEDNWENPTLGAWGLGWEVWLNGMEVTQFTYFQQVGGLECKPVT
                     GEITYGLERLAMYIQGVDSVYDLVWSDGPLGKTTYGDVFHQNEVEQSTYNFEYADVDF
                     LFTCFEQYEKEAQQLLALENPLPLPAYERILKAAHSFNLLDARKAISVTERQRYILRI
                     RTLTKAVAEAYYASREALGFPMCNKDK"
     gene            complement(3588..3887)
                     /gene="ysaB"
                     /locus_tag="ACHOSC_RS20355"
                     /old_locus_tag="pgaptmp_004071"
     CDS             complement(3588..3887)
                     /gene="ysaB"
                     /locus_tag="ACHOSC_RS20355"
                     /old_locus_tag="pgaptmp_004071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YsaB family lipoprotein"
                     /protein_id="HGU4549900.1"
                     /translation="MMMNAFFPAMALMVLVGCSTPSPVQKAQRVKVDPLRSLSMEALC
                     KDQAAKRYNTGEQKIDVTAFEQFQGSYEMRGYTFRKEQFVCSFDADGHFLHLSMR"
     gene            4062..5057
                     /gene="wecH"
                     /locus_tag="ACHOSC_RS20360"
                     /old_locus_tag="pgaptmp_004072"
     CDS             4062..5057
                     /gene="wecH"
                     /locus_tag="ACHOSC_RS20360"
                     /old_locus_tag="pgaptmp_004072"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-acetyltransferase WecH"
                     /protein_id="HGU4549901.1"
                     /translation="MQPKIYWIDNLRGIACLMVVMIHTTTWYVTNAHSVSPVTWDIAN
                     VLNSASRVSVPLFFMISGYLFFGERSAQPRHFLRIGLFLIFYSAIALLYIALFTSINM
                     ELALKNLLQKPVFYHLWFFFAIAVIYLVSPLIQVKNVGGKMLLVLMVVIGIIANPNTV
                     PQKIDGFEWLPINLYINGDTFYYILYGMLGRAIGMMDTQHKALSWVSAALFATGVFII
                     SRGTLYELQWRGNFADTWYLYCGPMVFICAIALLTLVKNTLDTRTIRGLGLISRHSLG
                     IYGFHALIIHALRTRGIELKNWPILDIIWIFCATLAASLLLSMLVQRIDRNRLVS"
     gene            complement(5099..5536)
                     /gene="yiaA"
                     /locus_tag="ACHOSC_RS20365"
                     /old_locus_tag="pgaptmp_004073"
     CDS             complement(5099..5536)
                     /gene="yiaA"
                     /locus_tag="ACHOSC_RS20365"
                     /old_locus_tag="pgaptmp_004073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312472.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inner membrane protein YiaA"
                     /protein_id="HGU4549902.1"
                     /translation="MDNKISTYSPAFSIVSWIALVGGIVTYLLGLWNAEMQLNEKGYY
                     FAVLVLGLFSAASYQKTVRDKYEGIPTTSIYYMTCLTVFIISVALLMVGLWNATLLLS
                     EKGFYGLAFFLSLFGAVAVQKNIRDTGINPPKETQVTQEEYSE"
     gene            complement(5582..5923)
                     /gene="yiaB"
                     /locus_tag="ACHOSC_RS20370"
                     /old_locus_tag="pgaptmp_004074"
     CDS             complement(5582..5923)
                     /gene="yiaB"
                     /locus_tag="ACHOSC_RS20370"
                     /old_locus_tag="pgaptmp_004074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709350.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inner membrane protein YiaB"
                     /protein_id="HGU4549903.1"
                     /translation="MKTSKTVAKLLFVVGALVYLVGLWISCPLLSGKGYFLGVLMTAT
                     FGNYAYLRAEKLGQLDNFFTHICQLVALITIGLLFIGVLNAPINAYEMVIYPIAFFVC
                     LFGQMRLFRSV"
BASE COUNT         1491 a         1525 c         1440 g         1542 t
ORIGIN      
        1 gggaataact tcccgccgaa gactcttact cttttaaatt gcaggctaaa aatgccgcca
       61 gctcataact cccctgttta atatgcaatt cacacagtga atctcttatc atccaggtga
      121 aaaataaaag cgtgaaacaa atcactatta aagaaagtaa tctatatttc tgcggcattc
      181 ccagccccct gtgttgattt ccaacgagta agaggctaac cttatggtgt tcaggcatag
      241 ggtagcctca ctttgattta tagtcaggtg gggcttttct ctgtctgcct ttcggtgaat
      301 acctgagaca aacagtctca agcacccgtg gctattctag cttaataagt ttgtttcttc
      361 tccttgatat aatcctaaaa aacctcataa aattaatata tgagataatc tttattcagc
      421 agaagattat taaaggttgc tgtattattt agcgataata aaaaaagcct gccagatggc
      481 aggcttttta ataacggcgt tattactgca acagcgaaat atccgcaacg cgcaggaaca
      541 gttcgcgcag tttctccagc atggtcagac ggttgagacg caattctttg tcatcaacca
      601 tgaccatcac tttatcgaag aaagcatcaa ccggctcacg cagctctgcc agttcgacca
      661 gcgcatcctg gtaacgacct tccgcaaagt acggctccag cttgtcacgt agcaccacaa
      721 cctgcatcgc cagtttaatt tcttccggct ctttcagggt agaggcattc acgcggtcgc
      781 tcagcacttc gtcagatttc gccaaaatgt tggagacacg cttgttcgcc gccgccagtg
      841 cagcagctgc ttcaagggta cggaagtgcg ataccgcttt catgcgggca tcgaaatcag
      901 ccggacgagt cggacgacgc gccagtaccg cctggatggt gtcaacggtg taaccttcgt
      961 cctgatacca ggcgcggaag cgaccgagca taaagtcgat aacatcatca accacgttgg
     1021 cgttagtcag cttatcgcca tacagacgca ctgcttcttc ggtcagcgtt tgcagatcaa
     1081 ggttgaggtt cttctcaacg ataattcgca gcacgccaag tgcggcacga cgcagcgcaa
     1141 acgggtcttt gtcgcctttc ggatgctggc cgataccgaa gatacccgcc agagtatcca
     1201 tcttgtcagc aatcgccagc gcacaggcta ccgggttaga cggcaggtca tcaccggcaa
     1261 agcgcggctg atactgctca ttcagcgcca ccgcgacatc ttccgcttcg ccatcgtgac
     1321 gcgcatagtg catccccata acgccctggg tgtcggtgaa ctcgaagacc atgttggtca
     1381 tcaggtcaca cttagacagc aggcccgcac gggttgcgtg gttaacgtca gcgccaatct
     1441 gttcagcaat ccagccagcc agcgcctgga tgcggtcagt tttgtcgcgc agcgtaccca
     1501 gctgttgctg gaacagcacg gtttgcaggc gcggcaggtt atcttccaga cgttttttac
     1561 ggtcggtatt gaagaagaac tcggcatccg ccagacgtgg acgaacgact ttctcgttac
     1621 cagagataat ttgctgcgga tctttcgatt cgatgttggc aacgaagatg aagttcggca
     1681 gcagtttgcc atcgttcgca tacaccggga agtatttctg gtcacctttc atggtgtaaa
     1741 ccagggcttc agccggcacc gcgaggaatt tctcttcgaa ttttgcggtc agaacgaccg
     1801 gccactccac cagcgaagcc acttcttcca gcaggctttc gcttaagtca gcgttaccgc
     1861 caatcttacg cgctgcttct tcggcatcgg ctttaatctt cgctttacgt tcttcgtaat
     1921 cggcgatgac tttcccgcgc tcacgcagaa tttccggata ctgatcggcg ttgtcgatgg
     1981 tgaattccgg ttcgcccata aagcggtggc cgcgaatcac gcgatcggac tgaatgccca
     2041 gaatggttgc cggaatgact ttgtcgccca gcagcagggt cacggtgtgc accggacgca
     2101 cgaagtgcac gtcgcttgcg ccccaacgca tcagtttcgg gatcggcagt ttcgccagag
     2161 aagtcgcaac catattcggc agcagtgctt cggtgctttc gcccttcaca tgggcgcgat
     2221 acagcagcca ttcgccttta tcggtagtca gacgctcagc ctggtcaacg gtaataccgc
     2281 aaccacgcgc ccaaccttct gccgctttgc tcggtttgcc ttcagcgtcg aacgcctggg
     2341 caatcgccgg gccgcgtttt tcgatttcac gatccggttg cgcttccgcc aggttagcta
     2401 ctttcagcgc cagacgacgc ggagcagcaa accattgaac ggtgccgtgt gcgaggccag
     2461 cgttatccag ctccgcagta aaattcgcag caaaggactc agccaggctg cgcagtgctt
     2521 ttggtggcag ctcttcagtg ccgatttcca ccagaaaagt tttctcagac atagccgcct
     2581 cttacttatc tttgttgcac atcgggaagc cgagggcttc acgggaagcg tagtatgctt
     2641 ctgccactgc tttggtcagg gtgcgaatgc gcagaatgta gcgctgacgc tcggtgacgg
     2701 agatggcttt acgcgcatcc agcaggttga agctgtgggc ggctttcaga atacgctcgt
     2761 aggctggcag cggcagcgga ttttccagcg ccagcagctg ctgcgcttct ttctcgtact
     2821 gctcaaagca ggtgaacagg aagtccacat ccgcgtattc gaagttgtaa gtggactgct
     2881 ccacttcgtt ctgatggaac acgtcgccgt aggtggtttt acccagcggg ccgtcgctcc
     2941 agaccaggtc gtaaacgctg tctacgccct gaatgtacat ggccagacgt tccagaccgt
     3001 aggtgatctc gccggtaacc ggtttacact ccagaccacc aacctgctgg aagtaagtga
     3061 actgcgtcac ttccatgccg tttagccaca cttcccagcc cagtccccag gcacccagag
     3121 tcgggttttc ccagttatct tccacgaaac ggatgtcgtg aatagtcggg tccatgccca
     3181 gctctttcag agaaccgagg tacagctcct gaatattgtc cggcgatggc ttaatgacca
     3241 cctggaactg atagtagtgc tgtaaacggt tggggttttc gccgtagcga ccatcggtcg
     3301 ggcgacgaga aggctgaaca taagcagccg ccatcggttc tggccccagc gcgcgcagac
     3361 aggtcattgg gtgagaggtt cccgcgccga cttccatgtc caatggttga acaatggtgc
     3421 agccctggcg agcccagtaa tcctgtaagg tcaggatcaa gccctggaag gtcctggtat
     3481 caaacttttg catattattt cgtgctggat acgtgtggat ttaaaggaag ggatcagtat
     3541 acccgctgga tggaagatat acagtacgaa acgggaaaaa gcagggctta acgcatggaa
     3601 agatgcaaaa aatggccatc tgcgtcaaaa gaacagacaa actgctcttt acggaaggta
     3661 taaccgcgca tttcatagct tccctggaac tgttcgaagg cggtgacgtc gattttttgc
     3721 tcgccggtgt tataacgttt tgccgcctga tccttgcata acgcttccat actcaacgaa
     3781 cgcagaggat caacctttac ccgttgtgct ttctgcacgg gtgacggtgt agaacaaccc
     3841 actagcacca taagcgccat tgccggaaag aaagcgttca tcatcattaa attaccgtct
     3901 ggtttgcagg tgagtcttat tatttatatc ggtatagtgg ctgatatctt acgaatttcc
     3961 ttaagcctct ataagctgta gtgataaaac tcagattatt ccagtgcgcc aaccaaatga
     4021 tttatcggtc agactttttt taaccagata cagagacaca gatgcagccc aaaatttact
     4081 ggattgataa cctgcgaggg atagcgtgtt taatggtggt gatgattcac accactacct
     4141 ggtatgtgac caatgctcat agtgttagcc ccgtcacatg ggatatcgcc aatgttctga
     4201 attctgcctc tcgtgtcagc gtgccgctat ttttcatgat ttccggctat ctcttttttg
     4261 gcgaacgcag cgcccagccg cgccatttct tgcgtatcgg cttatttctg attttttata
     4321 gcgcaatcgc actgctctac attgcgctgt ttacctccat caatatggag ttagcgctga
     4381 aaaacctgct gcaaaagcca gtgttttacc acttgtggtt tttcttcgcg attgcggtga
     4441 tttatctggt ttcaccgctg attcaggtga agaacgtcgg cggaaaaatg ttgctggtgc
     4501 taatggtggt gattggcatt atcgctaacc caaacacagt gccgcagaaa attgacggtt
     4561 ttgaatggct gccaattaac ttatatatca atggcgatac tttttactac attctgtatg
     4621 gcatgttggg ccgcgctata gggatgatgg acacacagca taaagcactg tcgtgggtga
     4681 gcgccgcgct gtttgcgacg ggggttttta ttatctctcg cgggacatta tatgaattgc
     4741 agtggcgcgg aaattttgcc gatacctggt atctttactg tgggccgatg gtttttatct
     4801 gcgcaatcgc gctattgact ctggttaaaa acacgctgga tacgcgtacc attcgcggac
     4861 ttggcttaat ctcccgccat tcattgggta tatacggatt ccacgccttg attatccatg
     4921 cgctgcgcac ccggggaatt gagcttaaaa attggccaat actggatatt atttggatct
     4981 tttgcgcgac gttggcagcg agtttgttac tttctatgct ggtacaacga atcgacagaa
     5041 acagattagt gagttaagta aaagcccgtt cacattggac tgaccgggct tacgtgaatt
     5101 attcgctgta ttcttcctgg gtaacctgtg tttcttttgg cgggtttatt ccggtatcac
     5161 gaatattctt ctgcaccgct actgcaccaa acaagcttaa gaagaaagcc agtccataaa
     5221 aacctttttc actgagtaat aatgtcgcat tccacagacc taccatcagt aatgcaacag
     5281 agataataaa gacagtcagg caggtcatat aataaatgga agtggtcggt atgccttcat
     5341 acttgtcccg aacggtcttt tgataagacg cagcagaaaa cagtcctaac accagtacgg
     5401 caaaataata ccctttttca tttaactgca tctctgcatt ccatagccct aacagatagg
     5461 taacgatacc accaacgaga gctatccatg acacaatact aaaggccggt gaataggttg
     5521 atattttgtt gtccatcaca gtattccttt cattcctgaa tgtgtaagag ctttatgttg
     5581 ctcataccga gcgaaacaaa cgcatttgac caaacaagca gacaaaaaag gcgatggggt
     5641 agatcaccat ttcataagca ttgataggtg catttaaaac accgataaac aagagaccga
     5701 tagtgattaa cgcaactaac tggcagatat gggtaaaaaa attatccagt tgcccgagtt
     5761 tttctgcgcg aagatatgca tagttaccaa aagttgctgt cattaacacg ccaagaaaat
     5821 agccttttcc acttagcaat gggcatgaga tccatagccc aaccagataa accagcgccc
     5881 cgacaacaaa taatagtttt gccacagttt ttgaggtttt cataacgatc tccatatcta
     5941 ccagcgatac attacgagta accaacgaaa gacaaaactg aaaaatgcca ttaacaaa
//
LOCUS       DAZKHF020000260         5962 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2201S31241
            SAMN43267800-rid25106413.denovo.262, whole genome shotgun sequence.
ACCESSION   DAZKHF020000260 DAZKHF020000000
VERSION     DAZKHF020000260.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267800
            Sequence Read Archive: SRR30307263
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5962)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5962)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 5962)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350581.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350581.2
            Genome Coverage       :: 151x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:21:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,652
            CDSs (total)                      :: 5,557
            Genes (coding)                    :: 5,320
            CDSs (with protein)               :: 5,320
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 237
            CDSs (without protein)            :: 237
            Pseudo Genes (ambiguous residues) :: 0 of 237
            Pseudo Genes (frameshifted)       :: 102 of 237
            Pseudo Genes (incomplete)         :: 130 of 237
            Pseudo Genes (internal stop)      :: 52 of 237
            Pseudo Genes (multiple problems)  :: 41 of 237
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5962
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267800-rid25106413.denovo.262"
                     /serotype="O 26:H11"
                     /isolate="2201S31241"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-01"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            150..878
                     /gene="gadW"
                     /locus_tag="ACHOSC_RS20375"
                     /old_locus_tag="pgaptmp_004075"
     CDS             150..878
                     /gene="gadW"
                     /locus_tag="ACHOSC_RS20375"
                     /old_locus_tag="pgaptmp_004075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid resistance transcriptional activator GadW"
                     /protein_id="HGU4549904.1"
                     /translation="MTHVCSVILIRRSFDIYHEQHKISLHNESIVLLEKNLADDFAFC
                     SPDTRRLDIDELTVCHYLQNIRQLPRNLGLHSKDRLLINQSPPMPLVTAIFDSFNESG
                     VNSPILSNMLYLSCLSMFSHKKELIPLLFNSISTVSGKVERLISFDIAKRWYLRDIAE
                     RMYTSESLIKKKLQDENTCFSKILLASRMSMARRLLELRQIPLHTIAEKCGYSSTSYF
                     INTFRQYYGVTPHQFAQHSPGTFS"
     gene            complement(1241..4354)
                     /gene="mdtF"
                     /locus_tag="ACHOSC_RS20380"
                     /old_locus_tag="pgaptmp_004076"
     CDS             complement(1241..4354)
                     /gene="mdtF"
                     /locus_tag="ACHOSC_RS20380"
                     /old_locus_tag="pgaptmp_004076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux pump RND permease MdtF"
                     /protein_id="HGU4549905.1"
                     /translation="MANYFIDRPVFAWVLAIIMMLAGGLAIMNLPVAQYPQIAPPTIT
                     VSATYPGADAQTVEDSVTQVIEQNMNGLDGLMYMSSTSDAAGNASITLTFETGTSPDI
                     AQVQVQNKLQLAMPSLPEAVQQQGISVDKSSSNILMVAAFISDNGSLNQYDIADYVAS
                     NIKDPLSRTAGVGSVQLFGSEYAMRIWLDPQKLNKYNLVPSDVISQIKVQNNQISGGQ
                     LGGMPQAADQQLNASIIVQTRLQTPEEFGKILLKVQQDGSQVLLRDVARVELGAEDYS
                     TVARYNGKPAAGIAIKLATGANALDTSRAVKEELNRLSAYFPASLKTVYPYDTTPFIE
                     ISIQEVFKTLVEAIILVFLVMYLFLQNFRATIIPTIAVPVVILGTFAILSAVGFTINT
                     LTMFGMVLAIGLLVDDAIVVVENVERVIAEDKLPPKEATHKSMGQIQRALVGIAVVLS
                     AVFMPMAFMSGATGEIYRQFSITLISSMLLSVFVAMSLTPALCATILKAAPEGGHKPN
                     ALFARFNTLFEKSTQHYTDSTRSLLRCTGRYMVVYLLICAGMAVLFLRTPTSFLPEED
                     QGVFMTTAQLPSGATMVNTTKVLQQVTDYYLTKEKDNVQSVFTVGGFGFSGQGQNNGL
                     AFISLKPWSERVGEENSVTAIIQRAMIALSSINKAVVFPFNLPAVAELGTASGFDMEL
                     LDNGNLGHEKLTQARNELLSLAAQSPDQVTGVRPNGLEDTPMFKVNVNAAKAEAMGVA
                     LSDINQTISTAFGSSYVNDFLNQGRVKKVYVQAGTPFRMLPDNINQWYVRNASGTMAP
                     LSAYSSTEWTYGSPRLERYNGIPSMEILGEAAAGKSTGDAMKFMADLVAKLPAGVGYS
                     WTGLSYQEALSSNQAPALYAISLVVVFLALAALYESWSIPFSVMLVVPLGVVGALLAT
                     DLRGLSNDVYFQVGLLTTIGLSAKNAILIVEFAVEMMQKEGKTPIEAIIEAARMRLRP
                     ILMTSLAFILGVLPLVISHGAGSGAQNAVGTGVMGGMFAATVLAIYFVPVFFVVVEHL
                     FARFKKA"
     gene            complement(4379..5488)
                     /gene="mdtE"
                     /locus_tag="ACHOSC_RS20385"
                     /old_locus_tag="pgaptmp_004077"
     CDS             complement(4379..5488)
                     /gene="mdtE"
                     /locus_tag="ACHOSC_RS20385"
                     /old_locus_tag="pgaptmp_004077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015870489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug transporter subunit MdtE"
                     /protein_id="HGU4549906.1"
                     /translation="MLTACDDKSAENAAAMTPEVGVVTLSPGSVNVLSELPGRTVPYE
                     VAEIRPQVGGIIIKRNFIEGDKVNQGDSLYQIDPAPLQAELNSAKGSLAKALSTASNA
                     RITFNRQASLLKTNYVSRQDYDTARTQLNEAEANVTVAKAAVEQATINLQYANVTSPI
                     TGVSGKSSVTVGALVTANQADSLVTVQRLDPIYVDLTQSVQDFLRMKEEVASGQIKQV
                     QGSTPVQLNLENGKRYSQTGTLKFSDPTVDETTGSVTLRAIFPNPNGDLLPGMYVTAL
                     VDEGSRQNVLLVPQEGVTHNAQGKATALILDKDDVVQLREIEASKAIGDQWVVTSGLQ
                     AGDRVIVSGLQRIRPGIKARAISSSQENASTESKQ"
     gene            5596..5874
                     /locus_tag="ACHOSC_RS20390"
                     /old_locus_tag="pgaptmp_004078"
     CDS             5596..5874
                     /locus_tag="ACHOSC_RS20390"
                     /old_locus_tag="pgaptmp_004078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4549907.1"
                     /translation="MRDLQTSGIVGLSASKVGYRHSDRHGEVNDKKNVAVLPTVPASI
                     RATKGSTGGYTYQGNKVVLASPLVITGGNEISICIPRHASHQFQLLMS"
     gene            complement(5875..>5962)
                     /locus_tag="ACHOSC_RS20395"
                     /old_locus_tag="pgaptmp_004079"
     CDS             complement(5875..>5962)
                     /locus_tag="ACHOSC_RS20395"
                     /old_locus_tag="pgaptmp_004079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="LuxR family transcriptional regulator"
                     /protein_id="HGU4549908.1"
                     /translation="KIQKFNIMYKLKLRRMSDIVTLGITSYF"
BASE COUNT         1467 a         1492 c         1503 g         1500 t
ORIGIN      
        1 attatcaatt aactgttatg caaaactact ttgtggataa attttggtcc taccaaatct
       61 ggcagttttt gcgctaagaa acagtctggc aacatttcat tagtatactg aaattgaaat
      121 aatcgcagta tgaaatataa gggataatca tgactcatgt ctgctcggtg atcctcattc
      181 gtcgttcatt cgatatttat catgaacagc ataaaatatc gctgcataac gagagtatcg
      241 tgctgctgga gaaaaatttg gcagacgatt ttgcgttttg ttcaccggat acgcgacgac
      301 tggatatcga tgagctgaca gtttgccatt acttacaaaa tattcgtcag ctaccacgca
      361 atttagggtt acatagcaaa gaccgtctat taattaacca gtcacccccc atgccgctgg
      421 tgacggcgat ttttgatagc ttcaatgaat ccggggtaaa ttcaccgata ctgagcaata
      481 tgctctacct ttcctgttta tcgatgtttt ctcataagaa agaactgatc cccttacttt
      541 tcaatagcat cagcactgtt tcaggaaaag ttgaacgcct tattagcttt gatatcgcca
      601 aacgttggta tctgcgcgat atcgcggaaa gaatgtatac cagcgagagt ctcatcaaaa
      661 aaaagttgca ggatgaaaat acctgtttca gtaaaatatt actcgcttcc aggatgtcga
      721 tggccagacg attactcgag ttacgtcaaa ttcctctgca tactattgca gaaaaatgtg
      781 gctatagcag tacgtcgtac tttataaaca catttcgaca atattatggt gtaacgccac
      841 atcagtttgc gcaacattcg ccaggtacct tttcctgaca tattttgcat ttgaatattg
      901 gtcaggatct cacacctgct tcatgtgaaa ctcttccctg atgatttctg ccgggctacc
      961 ggctagttct ctttcgcagt ttgccaggcg gtataggtat tttcaggagg aaaagactcg
     1021 gcatgtttgg gattattaag ctgacaattc atacgattac gggaatgtgg gtcagtattg
     1081 tgctgttcaa attgatgacg aatggctgga gtggtttcta ttttcagtgc tgtgtactaa
     1141 gcctggtatt cctgacggtt agctggttgt tgagtggtga atggttagca ggaaagagta
     1201 aggctgaacc ttcacgttca accttactct catttacacg ttacgctttt ttaaagcggg
     1261 caaagagatg ttccactaca acgaaaaaga ccggaacgaa gtaaattgcc agcactgttg
     1321 cggcaaacat cccgcccatc acgccggtac ctaccgcgtt ttgcgcgcca gaaccggcac
     1381 catgactgat aaccagcggc agcacgccga gaataaaggc cagagaggtc atcaggattg
     1441 ggcgtaaacg catccgcgcc gcttcgatga ttgcctctat cggcgttttc ccttctttct
     1501 gcatcatctc aacggcaaat tcgacaatca ggatggcgtt cttggcggaa agcccgatgg
     1561 tggtcagcaa accaacctgg aagtagacgt cattacttaa gccgcgcaga tcggtggcca
     1621 gtaatgcgcc aacgacgcct aacggaacaa ccaacatcac cgagaacgga attgaccagc
     1681 tctcatagag tgcggcgagg gcgaggaaca ccacgaccag tgaaatggca tacagtgcag
     1741 gagcctggtt tgaggataac gcttcctgat acgacagtcc ggtccatgag tagccgacgc
     1801 ctgccggaag tttagcgacc aggtctgcca taaatttcat agcgtcaccg gtacttttcc
     1861 cggccgccgc ttcacctaaa atctccattg acgggatgcc gttgtagcgt tccaatcgcg
     1921 gtgaaccata ggtccattcg gtagacgagt aggcagaaag cggtgccatc gtgccagagg
     1981 cgttgcgtac ataccattgg ttgatgttat ccggcaacat acggaacggc gtgcctgcct
     2041 ggacatacac ttttttcacc cgcccctggt tgaggaagtc gttcacgtag ctgctgccga
     2101 aggcggtgga aattgtctgg ttgatatcag acagcgccac acccattgcc tcagctttcg
     2161 cggcgttgac gttcactttg aacatcggcg tatcttccag gccgttcggg cgtaccccgg
     2221 tgacctggtc aggcgattgc gctgccagtg ataacagctc gtttcgtgcc tgggttagtt
     2281 tttcgtgccc caggttaccg ttgtccagca gttccatatc aaaacctgac gcggtaccca
     2341 gttcagccac cgcgggtaag ttgaacggga agacgacggc tttattgata ctgcttaacg
     2401 caatcattgc ccgctgaatg atcgcggtaa ccgagttttc ctcaccgaca cgttcagacc
     2461 acggcttgag actgataaac gccaggccgt tgttttgccc ctggccgctg aagccaaagc
     2521 cgccaacggt aaacaccgac tgaacattat ctttctcttt tgtaagataa taatccgtca
     2581 cctgttgcag cactttcgtg gtgttaacca tggtggcacc ggaaggtaac tgcgcggtgg
     2641 tcataaatac cccctgatcc tcttctggta agaaagaggt cggtgtacgc aggaacagca
     2701 ccgccatccc ggcgcaaatc agcaggtaga ccaccatgta gcgaccggta caacgcaaca
     2761 gcgagcgggt gctatcggta tagtgctgag ttgatttttc aaacagcgtg ttgaagcgtg
     2821 cgaacagggc gttaggtttg tgaccgcctt ccggcgcggc tttcagaatg gtggcgcaca
     2881 gggcaggggt caggctcatt gccacaaata ctgaaagcag catggaggag atcagcgtga
     2941 tggagaactg gcggtagatc tccccggttg cgccgctcat aaaggccatc ggcataaaca
     3001 ctgcggaaag aacaacagca ataccgacca gcgcacgttg gatctgcccc atcgatttat
     3061 gcgtcgcttc cttcggtggt agcttatctt ccgcaatgac acgctcgacg ttctccacca
     3121 ccacgatggc gtcatccacc agtaacccta tcgccagcac catcccgaac atagttaacg
     3181 tgttgatggt gaaaccgacc gccgacaaga tcgcaaacgt cccgagaata accaccggta
     3241 cggcaatcgt cgggatgatt gtggcacgga aattctgcaa aaacagatac atgaccagga
     3301 agacgaggat gatagcctca accagtgttt taaaaacttc ctgaatagaa atttcgataa
     3361 acggcgtggt gtcgtaagga taaaccgtct tcagacttgc ggggaaatag gctgataagc
     3421 ggttcagctc ctctttgact gcccgcgagg tatccagggc gtttgctccg gtagccagtt
     3481 tgatggcgat cccggcagca ggtttgccgt tatagcgcgc cacggtggaa taatcttccg
     3541 ctccaagttc gacgcgggcg acatcacgca gcagcacttg cgaaccatct tgctgaactt
     3601 tcaacaggat tttgccaaat tcttccggcg tttgcagacg cgtctgcaca atgatcgagg
     3661 cgtttagttg ctggtctgcc gcctgtggca tgccacccag ttgaccaccg gaaatctggt
     3721 tgttttgcac cttaatctgg gaaataacat cggaaggtac cagattgtat ttattgagtt
     3781 tttgcgggtc cagccagata cgcatggcgt actcggaacc aaagagctgt acgctaccaa
     3841 cgcccgcggt acggcttagc gggtctttga tattagacgc tacatagtcc gcgatatcgt
     3901 actggttgag gctgccgtta tcagaaataa acgccgctac catcagaata ttactgctcg
     3961 acttatcaac gctaatcccc tgctgctgca ccgcttcagg taatgaaggc atagcgagtt
     4021 gcagtttatt ttgcacctga acctgtgcga tatcaggaga tgtcccagtc tcgaaggtca
     4081 gagtgataga ggcattaccc gccgcatcac tggttgaaga catgtacatc aggccatcaa
     4141 gcccattcat attttgctca atcacctgag tgaccgagtc ttctaccgtt tgcgcatcgg
     4201 cacctggata ggtagcgctg acggtaatgg tcggtggcgc aatctgcgga tactgcgcaa
     4261 ccggtaagtt catgatcgcc agaccacctg caagcatcat aataatggca agtacccagg
     4321 caaaaaccgg gcgatcaata aaatagttag ccatgaaagt ccccttaagc ctgcaacgtt
     4381 attgtttcga ttcggtgctg gcgttttcct ggctggagga aattgctcgt gctttgatac
     4441 ccggacgaat gcgttgcaaa ccggaaacga tcacccgatc gccagcctgc aagccagagg
     4501 tgacaaccca ctggtcgccg atggctttgc tggcttcaat ttcgcgtagc tgcacaacat
     4561 cgtctttatc cagaatgagc gccgttgctt taccctgggc gttgtgggtg acgccttcct
     4621 gcggcaccag taatacattc tggcggctac cttcatccac taatgccgtg acgtacatgc
     4681 caggcagcaa gtcaccattt gggttgggga aaatcgcccg taacgtcacg gagcccgtgg
     4741 tttcatccac cgtcgggtcg gagaatttca gcgtgccggt ctggctgtag cgtttaccat
     4801 tttccagatt gagctgtact ggcgtactgc cctgaacctg tttgatttgc ccactggcga
     4861 cctcttcttt catgcgtaag aaatcttgca ccgactgcgt gagatcgaca taaatcgggt
     4921 ccagacgttg tacggtaacc agcgaatctg cctgattagc ggtaacgagt gcgccgacgg
     4981 tcaccgacga tttcccgctg acgcccgtaa tcggcgaggt gacattcgcg tattgcagat
     5041 tgatcgtcgc ctgttcaaca gccgctttgg cgacggtgac attggcttct gcttcattca
     5101 actgggtgcg cgcggtatcg taatcctgac ggctaacgta gttggtcttc agcaacgatg
     5161 cctggcggtt aaaggtgatg cgggcattgc tggcggtaga gagcgctttc gccagcgagc
     5221 ctttggcgga gtttagctcg gcctgtaaag gtgcaggatc aatctgatac agcgaatcgc
     5281 cctggttcac tttatcgcct tcgataaagt tgcgtttaat gataataccg cccacctggg
     5341 gacgtatctc ggcaacttca taaggaacgg ttctaccggg caattcgctc aacacattga
     5401 ccgaaccggg ggagagtgtg acgacaccga cctcaggcgt catggcggcg gcgttttccg
     5461 ccgatttgtc atcgcaggcg gtgagcatcg cgccgcagaa taacaacggt attaacagct
     5521 ttcttcttct gttcatttta gtccctgaaa attcttgaga aataacggtt ctttaaattg
     5581 cgcgcgagga gagatatgag ggatttacag accagcggca tagtggggtt gtcagccagt
     5641 aaggtgggat acaggcacag tgatcgacat ggtgaggtca acgacaaaaa aaatgttgcc
     5701 gttctgccaa cagttcctgc cagcattcgg gcaacgaagg gttctactgg tggatacaca
     5761 taccagggga ataaagtcgt tttagcctct cctttagtaa tcaccggggg taatgaaatc
     5821 agcatctgca tccctcgtca tgccagccat caatttcagt tgcttatgtc ctgactaaaa
     5881 ataagatgtg atacccaggg tgacgatgtc gctcatacgt cttagtttca gcttgtacat
     5941 aatgttgaat ttctgaatct tt
//
LOCUS       DAZKHF020000261         5879 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2201S31241
            SAMN43267800-rid25106413.denovo.263, whole genome shotgun sequence.
ACCESSION   DAZKHF020000261 DAZKHF020000000
VERSION     DAZKHF020000261.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267800
            Sequence Read Archive: SRR30307263
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5879)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5879)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 5879)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350581.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350581.2
            Genome Coverage       :: 151x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:21:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,652
            CDSs (total)                      :: 5,557
            Genes (coding)                    :: 5,320
            CDSs (with protein)               :: 5,320
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 237
            CDSs (without protein)            :: 237
            Pseudo Genes (ambiguous residues) :: 0 of 237
            Pseudo Genes (frameshifted)       :: 102 of 237
            Pseudo Genes (incomplete)         :: 130 of 237
            Pseudo Genes (internal stop)      :: 52 of 237
            Pseudo Genes (multiple problems)  :: 41 of 237
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5879
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267800-rid25106413.denovo.263"
                     /serotype="O 26:H11"
                     /isolate="2201S31241"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-01"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            147..494
                     /locus_tag="ACHOSC_RS20400"
                     /old_locus_tag="pgaptmp_004080"
     CDS             147..494
                     /locus_tag="ACHOSC_RS20400"
                     /old_locus_tag="pgaptmp_004080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000152742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4549909.1"
                     /translation="MTIKATDIEILHRYAEGVMERSNHHAKNVGAAALTLLGGVIWKA
                     LPGSIEIRTYNGSLANMVWWQSERTLKNYAISYNHNSCEIEMRDESVKGAVLFSISNE
                     TTPEKILSQLSEL"
     gene            complement(699..1400)
                     /locus_tag="ACHOSC_RS20405"
                     /old_locus_tag="pgaptmp_004081"
     CDS             complement(699..1400)
                     /locus_tag="ACHOSC_RS20405"
                     /old_locus_tag="pgaptmp_004081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replication protein P"
                     /protein_id="HGU4549910.1"
                     /translation="MKNIAAQMINFDREQMRRIANNMPEQYDEKPQVQQVAQIINGVF
                     SQLLATFPASLANRDQNELNEIRRQWVLAFRENGITTMEQVSAGMRVARRQNRPFLPS
                     PGQFVAWCREEASVNAGLPNVSELVDMVYEYCRKRGLYPDAESYPWKSNAHYWLVTNL
                     YQNMRANALTDAELRRKAADELTCMTARINRGETIPEPVKQLPVMGGRPLNRAQALAK
                     IAEIKAKFGLKGASV"
     gene            complement(1397..2416)
                     /locus_tag="ACHOSC_RS20410"
                     /old_locus_tag="pgaptmp_004082"
     CDS             complement(1397..2416)
                     /locus_tag="ACHOSC_RS20410"
                     /old_locus_tag="pgaptmp_004082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000147908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication protein"
                     /protein_id="HGU4549911.1"
                     /translation="MTGSEMAKAGLLEQNRLSGANRNTLIAGGIMANTAEIFNFPVPD
                     AAQKEPRVADLDDGYTRIANELLEAVMLAGLTQHQLLVFLAVMRKTYGFNKKLDWVSN
                     EQLSELTGILPHKCSAAKSVLVKRGIFIQSGRNIGINNVVSEWSTLPESGKKNKVYLK
                     EVNLPESGKKSLPKSGKGTYPNQVNTKDKLTKDNIKPYSSENSGESSDLPENDLPVVK
                     ADAAIQSGSKWGTAEDLTAAEWMFDMVKTIAPSARKPNFAGWANDIRLMRERDGRNHR
                     DMCVLFRWACQDNFWSGNVLSPAKLRDKWTQLEINRNKQQAGVTVGKPKLDLTNTDWI
                     YGVDL"
     gene            complement(2413..2952)
                     /locus_tag="ACHOSC_RS20415"
                     /old_locus_tag="pgaptmp_004083"
     CDS             complement(2413..2952)
                     /locus_tag="ACHOSC_RS20415"
                     /old_locus_tag="pgaptmp_004083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001182892.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxin YdaT domain-containing protein"
                     /protein_id="HGU4549912.1"
                     /translation="MQPLPYQQTSGFSPTAVINRSQTKQVPGHEKIRDAVRAWSAVDN
                     QDVVATLIVNEYREQGGGTIDFPDDVSRTRQKLFRFLDNKFDSEKYRNNVRELTPAIL
                     AVLPLEYRGYLVEQDSFMARLAEMEKELSEAKQAVILNAPRHQKLKEISEGIVSMFRV
                     DPELAGPLMAMVTTMLGAI"
     gene            complement(3022..3252)
                     /locus_tag="ACHOSC_RS20420"
                     /old_locus_tag="pgaptmp_004084"
     CDS             complement(3022..3252)
                     /locus_tag="ACHOSC_RS20420"
                     /old_locus_tag="pgaptmp_004084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001067458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HGU4549913.1"
                     /translation="MNPAIKTAINIVGSQKKLGAACEVSQQAVYKWLHNKAKVSPEHV
                     GSIVTATGGVVKAYQIRPDLPKLFPHTEKNAA"
     gene            3357..4046
                     /locus_tag="ACHOSC_RS20425"
                     /old_locus_tag="pgaptmp_004085"
     CDS             3357..4046
                     /locus_tag="ACHOSC_RS20425"
                     /old_locus_tag="pgaptmp_004085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000858971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S24 family peptidase"
                     /protein_id="HGU4549914.1"
                     /translation="MKTTLSERLKEARLARGLTQKALGDLVGVSQAAIQKIETGKANQ
                     TTKIVEIANALGVRAEWLSSGVGNMSDSTVQPIQSTVSHSKYFKIDVLDIEVSAGPGV
                     INREFVEVLRSVEYSFDDARHMFDGRKAENIRIINVRGDSMSGTIEPGDLLFVDITVK
                     SFDGDGIYAFLYDDTAHVKRLQMMKDKLLVISDNKSYSPWDPIEKDEMNRVFIFGKVI
                     GSMPQTYRKHG"
     gene            4128..4391
                     /locus_tag="ACHOSC_RS20430"
                     /old_locus_tag="pgaptmp_004086"
     CDS             4128..4391
                     /locus_tag="ACHOSC_RS20430"
                     /old_locus_tag="pgaptmp_004086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000066829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4549915.1"
                     /translation="MSNKTLVKKSNNCVDAYADAFGYSSFGDGSQRLGSISFFHNVTE
                     WPVDESDDVENIKYNIATIRMPEELMLKLADFIRDQHDKAKTN"
     gene            4404..4847
                     /locus_tag="ACHOSC_RS20435"
                     /old_locus_tag="pgaptmp_004087"
     CDS             4404..4847
                     /locus_tag="ACHOSC_RS20435"
                     /old_locus_tag="pgaptmp_004087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001472849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4549916.1"
                     /translation="MKHDYYEAPNLNDKIAKAAESFCLHSSASSQLAGISGKRWDMLQ
                     SSGSSGSGSDNMPEKVAKLESDVAHIKRDVDELKTDVKAIDKNMITILARLDSIKESL
                     TNKPSSDAVDRKISDAKLAVLLGVPAIIAAGTGLYKLSMYFFFSA"
     gene            5398..5604
                     /locus_tag="ACHOSC_RS20440"
                     /old_locus_tag="pgaptmp_004088"
     CDS             5398..5604
                     /locus_tag="ACHOSC_RS20440"
                     /old_locus_tag="pgaptmp_004088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000233581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division inhibitor protein"
                     /protein_id="HGU4549917.1"
                     /translation="MVHQHYGTQTVNRGAVMPGMLVKRKDGTWTASANLRGRLYLHRG
                     IERTYTRDLLVEVFLDGRGNALNH"
     gene            5680..>5879
                     /locus_tag="ACHOSC_RS20445"
                     /old_locus_tag="pgaptmp_004089"
     CDS             5680..>5879
                     /locus_tag="ACHOSC_RS20445"
                     /old_locus_tag="pgaptmp_004089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309203.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0060703 - deoxyribonuclease inhibitor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="host-nuclease inhibitor Gam family protein"
                     /protein_id="HGU4549918.1"
                     /translation="MNAYYIQDRLEAQSWARHYQQIAREEKEAELADDMEKGLPQHLF
                     ESLCIDHLQRHGASKKAITRAF"
BASE COUNT         1563 a         1406 c         1306 g         1604 t
ORIGIN      
        1 caatcatgct aataatcatt gttgttccag ttccaaatgc agcgaaataa tcaccaatat
       61 tttttctacc ggtttcgact ttgatgctat attctgctaa cacctaacta acactacgtt
      121 tcacacagca attacatgga aataacatga ctatcaaagc gactgacata gagattcttc
      181 atcgttatgc tgaaggggta atggaacgtt ctaaccatca tgcaaaaaat gttggtgcag
      241 ccgctcttac attgctagga ggtgttatct ggaaagccct gcccggatca atcgagataa
      301 gaacgtataa cggaagtctt gcaaatatgg tgtggtggca aagcgaaaga acattaaaaa
      361 attatgcaat ctcatacaac cataattcat gtgaaattga aatgagagat gaatcagtca
      421 aaggtgctgt attatttagc atttccaacg aaaccactcc agaaaaaata ttatcacagc
      481 tttctgagct ttaattaaag gcggtgctaa caccgcccat accattactc cacatcttac
      541 gcaacttgat aattatgctc tattaaatac ccacaatatt tcatcaatgc atgggtcagc
      601 tacaaaatgt ttaccgtggt aattcctgtt ttcgcaacat ctcaaaagaa caataaacac
      661 tgtatttctt caagtaacga agaatctttc tttgcccgtc atacacttgc tcctttcagc
      721 ccaaacttag ctttgatttc tgcgatcttc gccagagcct gtgcacgatt tagaggtcta
      781 ccgcccataa caggaagttg ttttactggt tcaggtatcg tctcaccacg gttaattcgc
      841 gctgtcatac aggtcagttc atcggcagcc ttgcgccgta attccgcgtc agtcagcgca
      901 ttggcccgca tgttctggta caggttggta accaaccagt aatgcgcgtt cgatttccac
      961 ggataagact ctgcatccgg atacaggcca cgcttccggc aatactcgta aaccatatca
     1021 accagctcgc tgacgtttgg cagcccggcg ttaacggatg cttcttcccg gcaccatgca
     1081 acaaactgcc cgggtgatgg cagaaatggt ctattctgcc gacgggcaac acgcattccg
     1141 gcgctcacct gttccatcgt ggtgatcccg ttttcccgga aagccagaac ccactggcgg
     1201 cggatttcgt tcagttcgtt ctggtcacga ttagccaggc tcgccgggaa agttgccagt
     1261 aactggctga atacaccgtt gattatctgc gctacctgct gtacctgcgg cttttcgtcg
     1321 tactgttccg gcatgttgtt ggcgatccga cgcatctgct cacggtcaaa gttaatcatc
     1381 tgtgcggcga tgtttttcat agatccaccc cgtaaatcca gtctgtgttt gtcaggtcga
     1441 gttttggttt gccgactgtc acgccagcct gttgcttgtt ccggttgatt tcgagctggg
     1501 tccacttgtc gcggagtttg gccggactca gcacgttacc ggaccagaag ttgtcctggc
     1561 aggcccagcg gaacagtaca cacatgtcgc ggtggttacg tccatcacgt tcacgcatca
     1621 gacggatatc gttagcccac cctgcaaaat tcggttttct ggctgatggc gcgatggtct
     1681 tcaccatgtc aaacatccac tctgcggcgg tcaggtcttc tgctgtcccc cacttgctgc
     1741 cgctctgaat cgcagcatcc gctttcacca caggaaggtc gttttctggc aggtcagagg
     1801 attcgccaga attctcggac gaataaggtt ttatattgtc ttttgttagt ttgtcttttg
     1861 tgtttacctg attcgggtaa gtgcctttac ctgatttggg taaacttttc ttacctgatt
     1921 caggtaaatt tacctctttc aggtaaactt tatttttctt acctgattcg ggtaatgttg
     1981 accattcact gaccacatta ttaatgccga tattccgccc gctctgaata aaaatcccac
     2041 gctttaccag aacacttttt gcagcagaac acttgtgcgg caatatcccg gtcaactcgg
     2101 aaagttgctc gttgctcacc cagtccagtt ttttattaaa gccatatgtt ttgcgcatga
     2161 cagccaggaa gaccagaagc tggtgctgtg ttaatccggc cagcattaca gcttccagca
     2221 actcatttgc aatgcgcgta taaccatcat cgagatctgc cacgcgcggc tccttttgtg
     2281 ccgcatccgg cactggaaaa ttgaatatct cagcagtgtt tgccataatt cctcccgcaa
     2341 tgagtgtgtt acgatttgca cctgaaagtc ggttctgttc cagcagaccg gctttcgcca
     2401 tttctgaacc tgtcatatcg cccccagcat ggtagtaacc atcgccatca atggaccagc
     2461 cagctctggg tccacacgaa acatcgacac aataccttca ctaatttcct tcagtttctg
     2521 gtggcgtggt gcgttgagaa tgacagcctg ttttgcctca ctgagttcct tttccatttc
     2581 agccaaccta gccatgaagc tatcctgctc aaccaggtaa ccgcgatatt ccagcggtag
     2641 taccgccaga attgccgggg tcagttcacg cacgttattt cggtattttt cagaatcgaa
     2701 tttgttatcg aggaagcgga acagcttctg gcgtgtacgg ctgacatcat cagggaaatc
     2761 gatggtgccg ccgccctgct cccgatactc attcacaatg agtgtggcaa cgacatcctg
     2821 attatctaca gccgaccagg cgcggacggc atcacggatt ttttcgtggc ctggcacctg
     2881 ttttgtttga gaacgattta tcaccgcagt cgggctaaat ccgctagtct gttggtatgg
     2941 aagtggttgc ataattgact cctttagttt gaattgactg ttaagttgat tgcttattgt
     3001 taaagagcgt gaaatggaaa tttaagctgc gttcttttcg gtgtgtggaa acaacttcgg
     3061 aagatccggg cgaatctggt atgccttcac tactccacca gtagccgtaa caatgctgcc
     3121 gacatgttca ggggatacct ttgctttgtt gtgaagccac ttatagacgg cctgctgtga
     3181 aacttcgcag gcagcgccca gtttcttttg tgaaccaacg atattgatcg ctgttttgat
     3241 agctgggttc ataacaacct ccgtggttaa tttgaatcaa gattaaaact atggttgttt
     3301 ttagtcaaca accattttcg tttgatggaa taaaaccttg gttgtacatt tggactatga
     3361 aaacaacact ctcagaaaga cttaaagaag ccagattagc gcgaggcctt acacaaaagg
     3421 cgcttgggga tttggtcggg gttagccagg ctgctattca gaaaatcgaa acagggaaag
     3481 ctaatcaaac aactaaaatc gtggagatcg cgaacgcttt gggtgtgcgc gcagaatggt
     3541 tatcttctgg cgttggaaat atgtcagaca gtacagtgca accaatacaa tcaactgtca
     3601 gccattccaa atacttcaag attgacgttc ttgatataga agtcagtgct gggccgggag
     3661 tcatcaaccg tgagtttgta gaagttctac gctcggttga gtactcgttt gacgatgctc
     3721 gtcacatgtt cgatggtagg aaggcagaaa atatccgcat cattaacgtg cgtggtgaca
     3781 gcatgtcagg aacgatcgaa ccaggtgatc tgctgttcgt tgatatcacg gttaaatctt
     3841 tcgacggtga tggtatctat gcatttctgt acgacgacac cgcccatgta aagcgcctgc
     3901 aaatgatgaa ggataaactg ctggttatct ctgataacaa gagctactca ccgtgggacc
     3961 ctatcgagaa agacgagatg aaccgggtat tcatattcgg taaggttatt gggagcatgc
     4021 cgcagacgta cagaaaacac ggataatcag ccgcgtgttg atgaggcttt tgggtaatac
     4081 gctgaagaaa ataccttata aaaaataaca ttacgggaaa ggcaaaaatg agtaataaaa
     4141 cacttgttaa aaaatcaaat aactgtgtcg atgcatacgc cgacgcgttt ggctactctt
     4201 cgtttggtga cggctctcag cgcctaggtt caatttcttt ttttcataat gtaactgaat
     4261 ggcctgttga tgaaagtgac gatgttgaga acataaagta caacattgca accattagaa
     4321 tgcccgaaga attgatgctt aaattggctg acttcattcg cgaccagcat gacaaagcta
     4381 aaacaaacta atcgtaatta attatgaaac acgattatta cgaagctcca aacctaaatg
     4441 ataaaattgc aaaggcggca gaatcatttt gtttgcactc cagtgcgtct tcccagctag
     4501 caggtatatc tggaaaaaga tgggacatgc tacaatcaag tggtagcagt gggtctggga
     4561 gtgataacat gcctgaaaaa gttgcaaaac tggagtccga tgttgctcat atcaaacgag
     4621 acgttgatga acttaaaaca gacgttaaag ctatcgacaa aaatatgata actattcttg
     4681 cacgtctgga ctctataaaa gaatccttaa caaacaagcc atcaagtgat gctgtcgata
     4741 gaaagatttc agacgcaaag cttgcggtat tgcttggtgt tccagcaatc atcgctgcag
     4801 gaacaggact ttataagcta tcaatgtact ttttctttag tgcttaagaa aatgctcgca
     4861 cccttgcatt aggtaaagcg atttttcatt ccccattacc ctcgctctta accaatcatc
     4921 taaacccggc caccgtgccg ggttttcttt tgccctcccc tcatcataca ccgttcaaaa
     4981 aaaccaccac gacctcgctt cagttatcgc tatgcgatgc aagtcacaaa ataaatccat
     5041 cttaaataca accagttata tctaaaacaa ccaacaaaac aacttttgtt gttgacgata
     5101 aaacaactat agttttaaat gagttcatcg caacaacaca acgatacggc aatcacctga
     5161 ttcaccgttg cgatgaccgc ttagatccgc agcttgaatt tcagcaggct tcggggagtg
     5221 cgagggatga aacggacgcg tgaacgtcgg tgtgaccagc tgaaatcaac tcaacatttc
     5281 ataccttagt cgcttcaacg aggcggctta gttatgacaa ccggcggcca tccaccgcct
     5341 gaatacgcgc agaagtcttt atatgttcag cagcccagct tacgggcagg agtttttatg
     5401 gttcatcaac attacggaac gcagaccgtt aatcgcggtg cggtcatgcc aggaatgctg
     5461 gtcaaacgca aagatggtac ctggactgca tcagctaatt tacgcggacg actttatctg
     5521 catcgcggca ttgagcgcac ttatacccgt gacttgctcg tggaagtttt tctcgacgga
     5581 cgcggcaacg ctctgaatca ctaatcccct ttcctgtttt ccgaatcagc ctggcattcc
     5641 gcgggcgatt ttttcacagc cattttcagg agttcagcca tgaacgctta ttacattcag
     5701 gatcgtcttg aggctcagag ctgggcgcgt cactaccagc agatcgcccg tgaagagaaa
     5761 gaggcagaac tggcagacga catggaaaaa ggcctgcccc agcacctgtt tgaatcgcta
     5821 tgcatcgatc atttgcaacg ccacggggcc agcaaaaaag ccattacccg tgcgtttga
//
LOCUS       DAZKHF020000262         5864 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2201S31241
            SAMN43267800-rid25106413.denovo.264, whole genome shotgun sequence.
ACCESSION   DAZKHF020000262 DAZKHF020000000
VERSION     DAZKHF020000262.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267800
            Sequence Read Archive: SRR30307263
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5864)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5864)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 5864)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350581.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350581.2
            Genome Coverage       :: 151x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:21:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,652
            CDSs (total)                      :: 5,557
            Genes (coding)                    :: 5,320
            CDSs (with protein)               :: 5,320
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 237
            CDSs (without protein)            :: 237
            Pseudo Genes (ambiguous residues) :: 0 of 237
            Pseudo Genes (frameshifted)       :: 102 of 237
            Pseudo Genes (incomplete)         :: 130 of 237
            Pseudo Genes (internal stop)      :: 52 of 237
            Pseudo Genes (multiple problems)  :: 41 of 237
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5864
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267800-rid25106413.denovo.264"
                     /serotype="O 26:H11"
                     /isolate="2201S31241"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-01"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            49..288
                     /locus_tag="ACHOSC_RS20450"
                     /old_locus_tag="pgaptmp_004090"
                     /pseudo
     CDS             49..288
                     /locus_tag="ACHOSC_RS20450"
                     /old_locus_tag="pgaptmp_004090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052685.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter outer membrane beta-barrel
                     domain-containing protein"
     gene            complement(336..953)
                     /gene="ureG"
                     /locus_tag="ACHOSC_RS20455"
                     /old_locus_tag="pgaptmp_004091"
     CDS             complement(336..953)
                     /gene="ureG"
                     /locus_tag="ACHOSC_RS20455"
                     /old_locus_tag="pgaptmp_004091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0016530 - metallochaperone activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0019627 - urea metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="urease accessory protein UreG"
                     /protein_id="HGU4549919.1"
                     /translation="MNIIKQPLRVGVGGPVGSGKTALLEALCKSMRDTWQLAVVTNDI
                     YTREDQRILTEAGALEAERIVGVETGGCPHTAIREDASMNLAAVEALSEKFGNLDLIF
                     VESGGDNLSATFSPELADLTIYVIDVAEGEKIPRKGGPGITKSDFLVINKTDLAPYVG
                     ASLEVMERDTLRMRGERPWGFTNLKSGEGLQNIIAFIEEQGMLGK"
     gene            complement(965..1639)
                     /locus_tag="ACHOSC_RS20460"
                     /old_locus_tag="pgaptmp_004092"
     CDS             complement(965..1639)
                     /locus_tag="ACHOSC_RS20460"
                     /old_locus_tag="pgaptmp_004092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="urease accessory protein UreF"
                     /protein_id="HGU4549920.1"
                     /translation="MPTPEKRLRLMQLASNSLPVGGYSWSQGLEWAVEAGWVEDSAAF
                     EHWQQLQMEQSFFAVDLPLLARLYRACEAGDPDSAGRWTAYLLACRETRELRDEERNR
                     GAAFTRLLVDWQPDCPAEWRKLCQQSQLTGMAWLGVRWQISVSDLALSLGYSWIESAV
                     MAGVRLVPYGQLAAQQLIMRLCARYAANMDSALATPDHAIGSATPLASIASARHETQY
                     SRLFRS"
     gene            complement(1640..2104)
                     /gene="ureE"
                     /locus_tag="ACHOSC_RS20465"
                     /old_locus_tag="pgaptmp_004093"
     CDS             complement(1640..2104)
                     /gene="ureE"
                     /locus_tag="ACHOSC_RS20465"
                     /old_locus_tag="pgaptmp_004093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016151 - nickel cation binding [Evidence
                     IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA];
                     GO_process: GO:0019627 - urea metabolic process [Evidence
                     IEA];
                     GO_process: GO:0065003 - protein-containing complex
                     assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="urease accessory protein UreE"
                     /protein_id="HGU4549921.1"
                     /translation="MLYLTRRVETPAQTTASVTLPVDMRVKSRIKVTLNDGRQAGLLL
                     PRGLLLRDGDILSNENGDEFIKVIAADEAVSVVRCADPFMLAKACWHLGNRHVPLQIM
                     PGELRYHHDHVLDDMLRQFGLDVDFAHLPFEPEAGAYASKSHAHNHDQEHSH"
     gene            complement(2114..3766)
                     /gene="ureC"
                     /locus_tag="ACHOSC_RS20470"
                     /old_locus_tag="pgaptmp_004094"
     CDS             complement(2114..3766)
                     /gene="ureC"
                     /locus_tag="ACHOSC_RS20470"
                     /old_locus_tag="pgaptmp_004094"
                     /EC_number="3.5.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309351.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009039 - urease activity [Evidence IEA];
                     GO_function: GO:0016151 - nickel cation binding [Evidence
                     IEA];
                     GO_process: GO:0019627 - urea metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="urease subunit alpha"
                     /protein_id="HGU4549922.1"
                     /translation="MFGPTTGDKIRLADTELWIEVEDDLTTYGEEVKFGGGKVIRDGM
                     GQGQMLSAGCADLVLTNALIIDYWGIVKADIGVKDGRIFAIGKAGNPDIQPNVTIPIG
                     VSTEIIAAEGRIVTAGGVDTHIHWICPQQAEEALTSGITTMIGGGTGPTAGSNATTCT
                     PGPWYIYQMLQAADSLPVNIGLLGKGNCSNPDALREQVAAGVIGLKIHEDWGATPAVI
                     NCALTVADEMDVQVALHSDTLNESGFVEDTLTAIGGRTIHTFHTEGAGGGHAPDIITA
                     CAHPNILPSSTNPTLPYTVNTIDEHLDMLMVCHHLDPDIAEDVAFAESRIRQETIAAE
                     DVLHDLGAFSQAMGRVGEVVLRTWQVAHRMKVQRGPLPEESGDNDNVRVKRYIAKYTI
                     NPALTHGIAHEVGSIEVGKLADLVLWSPAFFGVKPATIVKGGMIAMAPMGDINGSIPT
                     PQPVHYRPMFAALGSARHRCRVTFLSQAAAANGVAEQLNLHSTTAVVKGCRTVQKADM
                     RHNSLLPDITVDSQTYEVRINGELITSEPADILPMAQRYFLF"
     gene            complement(3792..4112)
                     /locus_tag="ACHOSC_RS20475"
                     /old_locus_tag="pgaptmp_004095"
     CDS             complement(3792..4112)
                     /locus_tag="ACHOSC_RS20475"
                     /old_locus_tag="pgaptmp_004095"
                     /EC_number="3.5.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0035550 - urease complex [Evidence IEA];
                     GO_process: GO:0043419 - urea catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="urease subunit beta"
                     /protein_id="HGU4549923.1"
                     /translation="MIPGEYKVKPGYIELNIGRATCSIIVENHGDRPIQVGSHYHFAE
                     VNPALKFDRQKARGYRLNIAAGTAVRFEPGQKREVDLVALSGARIVHGFRGDIMGELE
                     ANDE"
     gene            complement(4121..4423)
                     /locus_tag="ACHOSC_RS20480"
                     /old_locus_tag="pgaptmp_004096"
     CDS             complement(4121..4423)
                     /locus_tag="ACHOSC_RS20480"
                     /old_locus_tag="pgaptmp_004096"
                     /EC_number="3.5.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009039 - urease activity [Evidence IEA];
                     GO_function: GO:0016151 - nickel cation binding [Evidence
                     IEA];
                     GO_process: GO:0043419 - urea catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="urease subunit gamma"
                     /protein_id="HGU4549924.1"
                     /translation="MELTPREKDKLLLFTAALLAERRLARGLKLNYPESVALISAFIM
                     EGARDGKSVAALMEEGRHVLSREQVMEGIPEMIPDIQVEATFPDGSKLVTVHNPII"
     gene            complement(4433..5212)
                     /locus_tag="ACHOSC_RS20485"
                     /old_locus_tag="pgaptmp_004097"
     CDS             complement(4433..5212)
                     /locus_tag="ACHOSC_RS20485"
                     /old_locus_tag="pgaptmp_004097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008785843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="urease accessory protein UreD"
                     /protein_id="HGU4549925.1"
                     /translation="MLDLRFQRLHGKTTLTTRHHVGPLTVQRPFYPEEETCHLCLLHP
                     PGGIVGGDELIINAIIDSDCHTLITMPGASKFYRSSGAQAHLQQNLTLCNNATLEWLP
                     QDSIFFPGAHATLHTVFHLSSSSTLLAWDLLCLGRPVIGETFSHGTLANRLEIWVDGS
                     PLLIERLHVANGELTCVARKPWVGTMLFYLGNETQLEDIREKLTPLENYAGATLTDGL
                     LTVRFLSDDNLRCQQVMRDIWQFMRPQLTDKSPVLPRIWLT"
     gene            5593..>5864
                     /locus_tag="ACHOSC_RS20490"
                     /old_locus_tag="pgaptmp_004098"
     CDS             5593..>5864
                     /locus_tag="ACHOSC_RS20490"
                     /old_locus_tag="pgaptmp_004098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4549926.1"
                     /translation="MTEHWSGHADDNFPFSWGSDSIFMHACLQSYGESICDTVHTFVA
                     DVAPESLTGLQTVYLYTAGLLCDIVFHIEGGPPEMNGIIIFPDDIIP"
BASE COUNT         1318 a         1535 c         1577 g         1434 t
ORIGIN      
        1 ctgtagattc aatctgtcaa tgcaacaccc ctttcaatta tctctttcac tgcccgcgac
       61 ggtatcggtt atcagtttga cctgctggct aacggtgaaa ccgtactgcg tgatgcatcc
      121 ggcgaaaaac gcatcaaggg tgaaaaagac ggtcgtatgc tcatgaatgt tggcctgaat
      181 gcggaaattc gcgacaacat ccgcttcggt ctggagtttg aaaaatcggc atctggtaag
      241 tacaacgtgg ataacgcggt gaacgccaac ttccgttatt ccttctgata aaaacaaggg
      301 gccgcctgcc ggccctgata cagaggatgt tgtgattact tacccagcat gccctgctct
      361 tcgataaagg cgatgatgtt ttgcagacct tcaccacttt tcaggttagt gaacccccat
      421 gggcgttctc cgcgcatacg taaagtatca cgctccatta cctcgaggga ggcgcccacg
      481 tatggtgcaa ggtcagtttt attgatcacg aggaagtcgg atttggtgat ccccggaccg
      541 cctttacgcg ggatcttttc tccttctgcc acatctatga catatatggt aaggtccgcc
      601 agttcggggc tgaaggtcgc actcaggttg tcgccgccgc tttccacaaa gatcagatcg
      661 agattaccaa atttctcact cagtgcttcc acggctgcca gattcattga tgcgtcttca
      721 cgaatggctg tatgagggca gccccccgtt tcgacaccga caatgcgttc cgcttccagt
      781 gctccggcct cagtgagaat gcgttgatct tctctggtgt agatgtcgtt ggtgactacg
      841 gctaattgcc aggtgtcacg catggatttg cagagggctt ccagaagcgc ggttttacct
      901 gagccgaccg gaccgccgac gccgacacgc agcggttgct tgattatgtt cattttattt
      961 tctcctaaga acggaataat cgggaatact gggtttcatg tcgcgcggaa gcgatagatg
     1021 caagtggcgt tgctgaacca attgcgtgat ccggggttgc aagggcactg tccatgttcg
     1081 ccgcatagcg ggcacacagg cgcataatta actgctgggc agccagctgg ccgtagggta
     1141 ccagcctgac gcccgccatc actgcgcttt caatccagct gtagccaagg ctcagggcca
     1201 ggtctgaaac ggatatttgc cagcgtacgc cgagccatgc cataccggtt aactggcttt
     1261 gctggcatag tttgcgccat tcggccgggc agtcgggctg ccagtcgacc agaagtctgg
     1321 tgaaggctgc accgcggtta cgttcttcat cgcgtagctc acgggtttcc ctgcaggcca
     1381 gcagataagc ggtccagcgc ccggcactgt caggatcgcc cgcttcacag gcccggtata
     1441 gccgggcaag cagtggcagg tcgacggcaa aaaaactctg ttccatctgc aactgctgcc
     1501 agtgttcaaa ggcggcgcta tcttctaccc agcctgcttc cactgcccac tccagtccct
     1561 gggaccagct gtagcctcca acagggaggc tgttactggc aagctgcatc agccgcagac
     1621 gtttttccgg agtcggcatt cagtggctgt gctcctgatc gtgattatgg gcgtgcgact
     1681 tgctggcgta agcgccagct tccggctcaa acggaagatg tgcaaaatca acatccagcc
     1741 caaactggcg cagcatatca tcgaggacgt gatcgtgatg gtagcgcagc tctccgggca
     1801 ttatttgcag cggaacgtgg cgattgccga gatgccagca ggctttcgcg agcataaaag
     1861 gatctgcgca gcgcactaca gaaacggctt catcagcggc gatcacctta atgaattcat
     1921 caccgttttc gttactgaga atatcaccat cgcgcagcag cagaccgcgt ggcagcaaca
     1981 gtccagcctg acgtccatcg ttaagggtga ctttaatacg gctttttacc cgcatgtcaa
     2041 cagggagggt aacgctggca gttgtctgag ccggggtctc cacccgccgg gtcaaataaa
     2101 gcatttgtgc tccttaaaac aggaaataac gttgcgccat tggcagaatg tccgccggtt
     2161 cactggttat cagttcgccg ttgatacgca cttcgtaggt ttgtgaatcc acggttatat
     2221 caggcaacag gctgttgtgg cgcatatcgg ctttttgtac tgtgcggcag ccttttacca
     2281 cagcagttgt gctgtgcagg ttaagctgtt cagcgacgcc atttgctgct gctgcctgcg
     2341 acaggaaagt cacacgacag cggtgacggg cactgcccaa tgcagcgaac attgggcgat
     2401 agtgcaccgg ctgcggtgtg gggatagagc cgttgatatc acccatcggc gccatggcta
     2461 tcattccgcc tttgacgata gtcgccggtt ttacgccaaa gaacgccggg gaccagagca
     2521 ccagatccgc cagttttccc acttcaatcg agccgacttc atgagcaata ccgtgggtta
     2581 atgccggatt aatggtgtat ttagcgatat agcgcttcac gcggacgttg tcgttatcac
     2641 cactttcttc cggtaacggg ccgcgctgaa ctttcatccg gtgtgccacc tgccaggttc
     2701 gtaacactac ttctccgacg cgtcccatgg cctgggagaa cgcgccaagg tcgtgcagga
     2761 cgtcttccgc ggcaatggtt tcctggcgaa tgcgcgattc ggcaaaggct acgtcctcgg
     2821 cgatatccgg gtcaagatga tggcaaacca tcagcatgtc cagatgctca tcaatagtgt
     2881 tgacggtata gggtagcgtc ggattggttg aggaaggcag aatattgggg tgcgcgcagg
     2941 cggtgataat atccggagca tgaccaccac ctgcaccttc tgtatggaag gtatggatag
     3001 tgcgcccgcc gatggcagtc agagtatcct caacgaatcc tgattcgtta agcgtatcac
     3061 tgtgtagcgc aacctgaacg tccatttcgt cggctacagt cagtgcgcag ttgattaccg
     3121 caggtgtagc tccccagtct tcgtgaattt tgaggccgat aaccccggcc gcgacctgct
     3181 cacgaagcgc atccggattg gagcaattgc ctttacccag caacccgata ttgaccggca
     3241 ggctgtctgc agcctgcagc atttgataaa tgtaccatgg tcctggggta caggttgtgg
     3301 cgttagaacc cgctgtcggg ccagtaccgc caccgatcat ggtggtaatg ccggatgtca
     3361 gcgcttcttc agcctgctgt gggcagatcc agtgaatatg cgtatcgacg ccacctgcgg
     3421 taacgatcct gccttctgcg gcaataattt ccgtggatac gccgattggg atagtgacgt
     3481 tgggttgtat atcaggatta ccggctttgc cgatagcaaa tatccttcca tctttgacgc
     3541 cgatatcggc tttaacgatc ccccagtaat cgatgatcag ggcattggtc agcaccagat
     3601 cagcgcagcc ggcggagagc atttgcccct gtcccatacc gtcgcggatt actttaccgc
     3661 cgccgaattt gacctcttcg ccgtaggtag ttaaatcatc ttcgacctcg atccacagct
     3721 cagtgtctgc cagacgaatt ttatcaccgg tggtagggcc gaacatgtca gcataggcct
     3781 agcgtgaaat attactcatc atttgcctcc agctcaccca ttatatctcc gcggaaaccg
     3841 tgcacgatac gggcaccgct cagcgccacc agatcaacct cacgcttttg gcctggttcg
     3901 aagcgcactg cagttccggc ggcgatattg agacgatagc ctcttgcctt ctggcgatcg
     3961 aacttcagtg ccgggttaac ctccgcgaag tgataatggg aaccgacctg aattggtcgg
     4021 tctccgtgat tttcaacgat aatactgcag gtagcccggc cgatgttaag ctctatatag
     4081 ccaggtttaa ccttatattc tccagggatc atctgttacc tcagattatc ggattatgga
     4141 cggtaaccag cttggagccg tccggaaagg tggcttcgac ctggatatcg gggatcattt
     4201 ctggtatgcc ttccatgacc tgctcgcgac tcaggacatg ccgtccttct tccatcagcg
     4261 cagcgacgct tttgccgtcg cgagcgccct ccattataaa agcgctaatc agggccacgg
     4321 attcgggata gttaagtttc aggccgcggg ccagacgacg ctctgccagc agtgcagcgg
     4381 taaacagtaa tagtttgtct ttttctcttg gagtcagttc catagcgttc tcttaagtca
     4441 gccagatccg gggaagtacc ggtgatttgt cagtcagttg cggccgcata aattgccaga
     4501 tatcacgcat tacctgctgg caacgcagat tatcatcact taaaaagcga actgtcagta
     4561 gaccatcggt gagcgtggct ccggcgtagt tttccagtgg tgtgagtttt tcgcgtatgt
     4621 cttccagttg cgtttcattc cccaggtaga acagcatcgt tcccacccat ggtttccggg
     4681 caacgcaggt aagttctcca ttggccacgt gcagacgctc aatcaacagc ggagaaccgt
     4741 cgacccatat ctccagacgg ttagcgagtg ttccgtggct gaaggtttct ccgatgaccg
     4801 gacgtccaag gcacagaaga tcccaggcca gcagtgtact ggaggacgac agatgaaaca
     4861 cggtatgcaa cgtggcgtga gcgccgggaa aaaaaatcga atcctgtggc aaccactcca
     4921 gcgttgcgtt attgcacagc gtcaggtttt gctgaaggtg cgcctgcgcg ccgctgctgc
     4981 ggtaaaattt actggcacca ggcatggtga tcagcgtatg gcaatcagag tcaattattg
     5041 cgttgatgat gagttcgtca ccgccgacga taccaccggg tggatgaagc agacagaggt
     5101 ggcaggtttc ttcttcggga taaaaaggac gctgaacagt gagcggaccg acatgatgac
     5161 gggtggtgag cgtggttttc ccgtgcagac gctgaaaacg gagatccagc atggcctgcc
     5221 agcctttttt attttgtggt aacgcggtac tgagcaatct tacgcccctg ataaataaag
     5281 ataaaacgac agtaaagagc cggatttcct tgttatataa aagatgatgc ctttccagtg
     5341 agttttcgtc tgaataatac gaatgtttgt tttcgtcatg aagcgttccg gctcccgggg
     5401 ctatacatcg ccagaaaagc aatgcatatt gtcgggatta tccagagaat caggcagaaa
     5461 gatccgacaa tgggctgtga ttatgccatc tggctcagaa tgatatcaag gtgttatggg
     5521 gataacttgc tgatacaata gcagtatgac agattcatta atgcggaaac tgctcactga
     5581 aacagagtgt tcatgactga gcactggtcg ggtcatgcag atgataattt ccccttttct
     5641 tggggaagcg acagtatttt catgcatgcc tgtcttcagt catacgggga gagcatctgt
     5701 gatacagtcc atacatttgt tgcggacgtc gctcctgagt cactgactgg tctgcaaaca
     5761 gtttatctgt acacggcagg gcttctgtgt gatatcgttt ttcatatcga gggggggcct
     5821 ccagagatga atggaataat aatatttcct gatgatataa tacc
//
LOCUS       DAZKHF020000263         5820 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2201S31241
            SAMN43267800-rid25106413.denovo.265, whole genome shotgun sequence.
ACCESSION   DAZKHF020000263 DAZKHF020000000
VERSION     DAZKHF020000263.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267800
            Sequence Read Archive: SRR30307263
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5820)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5820)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 5820)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350581.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350581.2
            Genome Coverage       :: 151x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:21:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,652
            CDSs (total)                      :: 5,557
            Genes (coding)                    :: 5,320
            CDSs (with protein)               :: 5,320
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 237
            CDSs (without protein)            :: 237
            Pseudo Genes (ambiguous residues) :: 0 of 237
            Pseudo Genes (frameshifted)       :: 102 of 237
            Pseudo Genes (incomplete)         :: 130 of 237
            Pseudo Genes (internal stop)      :: 52 of 237
            Pseudo Genes (multiple problems)  :: 41 of 237
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5820
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267800-rid25106413.denovo.265"
                     /serotype="O 26:H11"
                     /isolate="2201S31241"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-01"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            <1..110
                     /locus_tag="ACHOSC_RS20495"
                     /old_locus_tag="pgaptmp_004099"
     CDS             <1..110
                     /locus_tag="ACHOSC_RS20495"
                     /old_locus_tag="pgaptmp_004099"
                     /EC_number="3.1.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308235.1"
                     /note="catalyzes the hydrolysis of S-D-lactoylglutathione
                     to D-lactic acid and reduced glutathione; plays an
                     important role in cellular detoxification using
                     glutathione; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hydroxyacylglutathione hydrolase"
                     /protein_id="HGU4549927.1"
                     /translation="LRTEDIDLINVINEETLLQQPEERFAWLRSKKDRF"
     gene            182..1540
                     /gene="mltD"
                     /locus_tag="ACHOSC_RS20500"
                     /old_locus_tag="pgaptmp_004100"
     CDS             182..1540
                     /gene="mltD"
                     /locus_tag="ACHOSC_RS20500"
                     /old_locus_tag="pgaptmp_004100"
                     /EC_number="4.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008933 - lytic transglycosylase activity
                     [Evidence IEA];
                     GO_process: GO:0000270 - peptidoglycan metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="murein transglycosylase D"
                     /protein_id="HGU4549928.1"
                     /translation="MKAKAILLASVLLVGCQSTGNVQQHAQSLSAAGQGEAAKFTSQA
                     RWMDDGTSIAPDGDLWAFIGDELKMGIPENDRIREQKQKYLRNKSYLHDVTLRAEPYM
                     YWIAGQVKKRNMPMELVLLPIVESAFDPHATSGANAAGIWQIIPSTGRNYGLKQTRNY
                     DARRDVVASTTAALNMMQRLNKMFDGDWLLTVAAYNSGEGRVMKAIKTNKARGKSTDF
                     WSLPLPQETKQYVPKMLALSDILKNSKRYGVRLPTTDESRALARVHLSSPVEMAKVAD
                     MAGISVSKLKTFNAGVKGSTLGASGPQYVMVPKKHADQLRESLASGEIAAVQSTLVAD
                     NTPLNSRVYTVRSGDTLSSIASRLGVSTKDLQQWNKLRGSKLKPGQSLTIGAGSSAQR
                     LANNSDSITYRVRKGDSLSSIAKRHGVNIKDVMRWNSDTANLQPGDKLTLFVKNNNMP
                     DS"
     gene            complement(1588..2358)
                     /locus_tag="ACHOSC_RS20505"
                     /old_locus_tag="pgaptmp_004101"
     CDS             complement(1588..2358)
                     /locus_tag="ACHOSC_RS20505"
                     /old_locus_tag="pgaptmp_004101"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HGU4549929.1"
                     /translation="MTIQSHHDHVEKQFSSQASEYLTSTVHASGRDLQRLAVRLADYP
                     GASVLDMGCGAGHASFVAAQNVSTVVAYDLSAHMLDVVAQAAEVRQLKNITTRQGYAE
                     SLPFADNAFDIVISRYSAHHWHDVGAALREVNRILKPGGRLIVMDVMSPGHPVRDIWL
                     QTVEALRDTSHVRNYASGEWLTLINEANLIVDNLITDKLPLEFSSWVVRMRTPEALID
                     AIRIYQQSASTEVRTYFALQNDGSFTSDIIMVEAHKAA"
     gene            complement(2436..3236)
                     /gene="yafD"
                     /locus_tag="ACHOSC_RS20510"
                     /old_locus_tag="pgaptmp_004102"
     CDS             complement(2436..3236)
                     /gene="yafD"
                     /locus_tag="ACHOSC_RS20510"
                     /old_locus_tag="pgaptmp_004102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HGU4549930.1"
                     /translation="MRKNTYAMRYVAGQPAERILPPGSFASIGQALPPGEPLSTEERI
                     RILVWNIYKQQRAEWLSVLKNYGKDAHLVLLQEAQTTPELVQFATANYLAADQVPAFV
                     LPQHPSGVMTLSAAHPVYCCPLREREPILRLAKSALVTVYPLPDTRLLMVVNIHAVNF
                     SLGVDVYSKQLLPIGDQIAHHSGPVIMAGDFNAWSRRRMNALYRFAREMSLRQVRFTD
                     DQRRRAFGRPLDFVFYRGLNVSEASVLVTRASDHNPLLVEFSPGKPDK"
     gene            3477..4391
                     /gene="yafC"
                     /locus_tag="ACHOSC_RS20515"
                     /old_locus_tag="pgaptmp_004103"
     CDS             3477..4391
                     /gene="yafC"
                     /locus_tag="ACHOSC_RS20515"
                     /old_locus_tag="pgaptmp_004103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator YafC"
                     /protein_id="HGU4549931.1"
                     /translation="MKATSEELAIFVSVVESGSFSRAAEQLGQANSAVSRAVKKLEMK
                     LGVSLLNRTTRQLSLTEEGQRYFRRVQSILQEMAAAESEIMETRNTPRGLLRIDAATP
                     VVLHFLMPLIKPFRERYPEVTLSLVSSETIINLIERKVDVAIRAGTLTDSSLRARPLF
                     NSYRKIIASPDYISRYGKPETIDDLKQHVCLGFTEPASLNTWPIACSDGQLHEVKYGL
                     SSNSGETLKQLCLSGNGIACLSDYMIDKEIVRGELVELMADKVLPVEMPFSAVYYSDR
                     AVSTRIRAFIDFLSEHVKTAPGGAVREA"
     gene            complement(4388..5191)
                     /gene="dkgB"
                     /locus_tag="ACHOSC_RS20520"
                     /old_locus_tag="pgaptmp_004104"
     CDS             complement(4388..5191)
                     /gene="dkgB"
                     /locus_tag="ACHOSC_RS20520"
                     /old_locus_tag="pgaptmp_004104"
                     /EC_number="1.1.1.346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047834 - D-threo-aldose 1-dehydrogenase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2,5-didehydrogluconate reductase DkgB"
                     /protein_id="HGU4549932.1"
                     /translation="MAIPAFGLGTFRLKDDVVISSVKTALELGYRAIDTAQIYDNEAA
                     VGQAIAESGVPRHELYITTKIWIENLSKDKLIPSLKESLQKLRTDYVDLTLIHWPSPN
                     DEVSVEEFMQALLEAKKQGLTREIGISNFTIPLMEKAIAVIGAENIATNQIELSPYLQ
                     NRKVVAWAKQHGIHITSYMTLAYGKALKDEVIARIAAKHNATPAQVILAWAMGEGYSV
                     IPSSTKRENLESNLKAQNLQLDAEDKKAIAALDCNDRLVSPEGLAPEWD"
     gene            complement(5354..5430)
                     /locus_tag="ACHOSC_RS20525"
                     /old_locus_tag="pgaptmp_004105"
     tRNA            complement(5354..5430)
                     /locus_tag="ACHOSC_RS20525"
                     /old_locus_tag="pgaptmp_004105"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(5394..5396),aa:Asp,seq:gtc)
     gene            complement(5624..5739)
                     /gene="rrf"
                     /locus_tag="ACHOSC_RS20530"
                     /old_locus_tag="pgaptmp_004106"
     rRNA            complement(5624..5739)
                     /gene="rrf"
                     /locus_tag="ACHOSC_RS20530"
                     /old_locus_tag="pgaptmp_004106"
                     /product="5S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00001"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00001"
BASE COUNT         1518 a         1406 c         1429 g         1467 t
ORIGIN      
        1 ttttaagaac ggaagatatt gatttaatta atgtaattaa tgaagaaaca ttattgcaac
       61 aacctgaaga gcgttttgca tggttaaggt caaagaaaga taggttctga taaaactttc
      121 ttgtcatcgg ctccgttcgc cgttatgatc ggtcgtcttt taagcaacta ttgacacaca
      181 catgaaggca aaagcgatat tactcgcctc tgtcctgctc gtgggttgcc agagtaccgg
      241 caacgttcaa cagcacgcac agagcctttc tgcagctggt caaggggaag cagcaaagtt
      301 tacaagtcag gcacgatgga tggacgatgg gacgtctatc gcgccagatg gtgacttgtg
      361 ggctttcatt ggcgacgagc taaagatggg aattccggaa aatgaccgga ttcgcgaaca
      421 gaaacagaaa tatttacgca ataagagcta tctccacgat gtaactttac gggcagagcc
      481 gtatatgtac tggatagcag ggcaagttaa aaaacgtaac atgcctatgg aactggtact
      541 actacccata gtggagagcg cttttgatcc tcacgcaacg tctggcgcca atgccgcagg
      601 catctggcag atcattccga gcacggggcg caattatggt ttgaaacaga cccgcaatta
      661 tgacgcgcgt cgcgatgttg ttgcttcaac aactgccgcg ctgaatatga tgcagcgtct
      721 gaacaagatg tttgacggcg actggcttct gaccgtagcg gcttataaca gcggcgaagg
      781 tcgagtcatg aaggcaatta aaacgaacaa agcgcgtggg aaatccacgg acttctggtc
      841 gttaccgttg ccgcaggaaa cgaagcagta cgtgcctaaa atgctggcat tgagtgatat
      901 tctcaaaaac agcaagcgtt atggcgtacg tctgccaacg accgatgaaa gccgtgctct
      961 ggcgcgtgtg cacctgagca gcccggttga aatggcgaag gttgcagata tggcggggat
     1021 ttccgtcagc aagctgaaga cattcaacgc tggcgtgaaa ggctccacgc tgggcgcaag
     1081 tggtccgcag tacgtgatgg tgccaaagaa gcatgcagat caactgcgtg aatctctggc
     1141 ttcaggcgaa attgctgctg tacagtcgac gctggttgcc gacaatacgc cgcttaacag
     1201 ccgtgtttac accgtacgct ctggcgacac gctttcaagt atcgcttcac gtctcggcgt
     1261 aagcaccaaa gatttgcagc agtggaacaa actgcgcgga tctaaactga agccaggcca
     1321 aagtttgacg attggcgcag gcagtagcgc acagcgactg gcaaacaaca gcgatagcat
     1381 tacgtatcgt gtgcgcaaag gcgattcgct ttcaagcatt gctaaacgcc acggcgtgaa
     1441 catcaaagat gtgatgcgct ggaacagcga cactgcgaat ctgcaaccag gcgataagct
     1501 gacgttgttt gtgaaaaaca acaacatgcc agattcctga caaaccagat aataaaaagg
     1561 caccgattcc cccggtgcct tttttattta tgccgcttta tgtgcttcta ccatgatgat
     1621 atcactggtg aaagagccgt cattctgcaa ggcaaaatac gttctcacct ctgtcgatgc
     1681 gctctgttgg taaatgcgaa tagcgtctat taacgcttct ggcgtacgca ttctcacgac
     1741 ccatgaagaa aattccagcg gtaacttatc tgtaattaaa ttatcaacta tcagattggc
     1801 ttcattgatt aacgtcaacc actcaccgct ggcgtagttt cgtacgtgag aggtatcgcg
     1861 taatgcttct accgtctgta accagatgtc gcgcactggg tgacccggag acattacgtc
     1921 catcacaatc agcctaccgc caggtttcaa tatccggttc acttcacgca gtgctgcacc
     1981 aacatcatgc cagtgatggg cagaataacg gctgataaca atatcaaatg cgttgtcggc
     2041 aaatggcaga ctttcggcat aaccctggcg ggtggtgata tttttcagtt gccggacttc
     2101 ggcagcttgt gccacgacat ctagcatgtg ggcagataag tcatacgcca ccaccgtgct
     2161 cacgttttgc gcagcgacaa agctggcatg ccctgctccg cagcccatat caagtacact
     2221 tgcaccagga taatcagcca gacgcaccgc caggcgctgt aaatctcgtc cggatgcatg
     2281 tacggtactg gtcagatatt cgctggcttg agagctaaac tgcttttcta catggtcatg
     2341 gtgggattgt attgtcattg cgctgtcctt ttgttatttg agtataaaag ggcagcgcca
     2401 aacgttggtc tgccctgtgg cagacctgac ataccttatt tatcaggctt gccgggactg
     2461 aattcaacga gtagcggatt gtgatcggaa gcgcgcgtaa ccagtacaga agcttcgctg
     2521 acgttcagac cacggtagaa aacaaaatcg agcgggcgac caaacgcccg acggcgctga
     2581 tcatcggtaa aacgcacctg gcgcagcgac atttcccgcg caaagcgata taacgcgttc
     2641 atccttctac ggctccaggc attgaaatct cccgccataa tgaccgggcc gctgtggtga
     2701 gctatctgat caccaatagg aagtaactgc ttactataga catccacgcc cagactgaag
     2761 ttgacggcgt gtatattaac caccatcaac aggcgggtgt caggtaatgg atagaccgtc
     2821 accagtgccg acttcgccag acgcaaaatg ggttctcgtt cacgtaacgg gcagcaatac
     2881 actggatgcg ccgccgaaag ggtcattaca ccggaaggat gttgtggcag cacgaaagcg
     2941 ggcacctgat cggcggcaag atagttagcg gtcgcaaact gtactaactc tggcgttgtc
     3001 tgcgcttcct gcaataacac cagatgtgca tctttgccgt agttctttaa taccgacaac
     3061 cattcagcgc gttgctgttt gtatatgttc cacaccagga tccgaatacg ctcttcagta
     3121 cttaacggtt ctccaggtgg taatgcctgg ccgatgctcg caaaagaccc cggcggtaag
     3181 atcctttccg caggttgtcc ggcaacatag cgcatggcat aggtgttttt tcgcactttt
     3241 gactttgaaa cctctattac gtcaaccctt ccgcactcgg gagggttcta ctgttatagg
     3301 gattttagct cactctttca acgagcaatt gtatattgtt atgtaagcaa gtgcttacac
     3361 aggtcagaat tttgctcatt attgagaaca ttaaatcgga tgacatatca cgcatcctga
     3421 tggtttaatg acaaataaat tcaaaatatc tcttctattt tgcaacagga gcaaaaatga
     3481 aagccacgtc ggaagaactc gccatttttg tttcggtcgt agaaagcggc agctttagcc
     3541 gggcagcgga acaattaggg caagcaaact cagcggtaag ccgggcagtg aaaaagctgg
     3601 agatgaaact tggcgttagc ctgcttaatc ggaccacgcg acaacttagc ctgacggaag
     3661 aaggccagcg ttattttcgt cgcgtacagt caattttgca ggagatggca gcggcagaat
     3721 cagaaattat ggagacgcgt aatacaccgc gtggactgtt acgcatcgat gccgcaactc
     3781 cagtggtgct gcactttctg atgccgttaa ttaagccttt ccgtgaacgc tatccggaag
     3841 ttactttatc gctagtctcc tccgaaacga ttattaattt gatcgaaaga aaagtggatg
     3901 tcgcgatacg cgctggtacg ttaacggatt ccagcttacg tgccaggccg ttatttaaca
     3961 gttatcgaaa aattatcgcc tcccccgatt atatttcccg ctacgggaag ccagaaacga
     4021 tcgacgattt aaagcaacat gtttgcctgg gattcactga acccgcttcc ctcaatacct
     4081 ggccgatagc ctgtagtgat ggacaattac atgaggtgaa gtacggtttg tcatccaata
     4141 gtggggaaac actgaaacag ctttgcctga gtggcaacgg gattgcgtgt ttgtccgact
     4201 acatgatcga caaagaaatc gttcgcggag aattggtgga gttaatggca gataaagtgt
     4261 tgccagtgga aatgccattc agtgcagtct attacagcga ccgtgcggta agtacgcgca
     4321 tccgggcttt tatcgatttc cttagcgagc atgtaaaaac agctcccgga ggagctgtca
     4381 gagaggctta atcccattca ggagccagac cttccgggct aaccaggcgg tcgttgcaat
     4441 ccagtgcggc gatcgctttt ttatcttcgg catcaagctg taaattttgt gccttaagat
     4501 tactttccag gttttcacgt ttagtagaag aaggaattac tgagtaacct tcccccatag
     4561 cccacgccag aatcacttgt gccggagtcg cattgtgttt agctgcgata cgagcaataa
     4621 cctcatcttt caaggctttc ccataagcca gcgtcatata ggaagtaata tggatgccgt
     4681 gctgtttagc ccaggcaacc actttacggt tttgcagata aggagagagt tcaatctggt
     4741 tagtagcgat gttttcagcg ccaataacag caatcgcctt ttccatcaat gggatcgtga
     4801 agttggaaat accgatctca cgcgtcagcc cttgtttttt ggcttccagc agcgcctgca
     4861 taaactcttc aacagagact tcatcgtttg gtgacggcca gtggattagc gtcagatcaa
     4921 cataatcggt acgcaatttt tgcaggctct ctttcagact tgggatcaat ttgtctttgc
     4981 tgagattttc aatccagatt ttagtggtga tgtagagttc atgacgtggc acgccacttt
     5041 ctgcaatcgc ctgacctact gcggcttcgt tatcatagat ttgtgcggta tcaattgcgc
     5101 gataaccaag ttcaagcgcc gttttcacag atgaaataac aacgtcgtct ttcagacgga
     5161 aagtacctaa accaaatgca gggatagcca taatatgcct cttttaatgc tattttcgtt
     5221 aactgaggtg attatgggct tcagactgtt gaggaaaaag agcgtaaaat gcagaggatt
     5281 tttgcgattc tggcaataat agatatccag acgaaaaaaa acctcgagcg ttaactcgag
     5341 gcttcttaat aagtggcgga acggacggga ctcgaacccg cgaccccctg cgtgacaggc
     5401 aggtattcta accgactgaa ctaccgctcc accgaattct tttacaacca ccggttttat
     5461 gaccggctta ctgcttaatt tgatgcctgg cagtttatga atcacttcgt gattcaccct
     5521 tcgggccgtt gcttcgcaac gttcaaaagc tttcgctttt gtcctactct cttatcgagt
     5581 agtgaactgc ctcaaagatc agtgccattc ttcgtaattt gatgcctggc agttccctac
     5641 tctcgcatgg ggagacccca cactaccatc ggcgctacgg cgtttcactt ctgagttcgg
     5701 catggggtca ggtgggacca ccgcgctaag gccgccaggc aaattctgtt ttatcagacc
     5761 gcttctgcgt tctgatttaa tctgtatcag gctgaaaatc ttctctcatc cgccaaaaca
//
LOCUS       DAZKHF020000264         5810 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2201S31241
            SAMN43267800-rid25106413.denovo.266, whole genome shotgun sequence.
ACCESSION   DAZKHF020000264 DAZKHF020000000
VERSION     DAZKHF020000264.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267800
            Sequence Read Archive: SRR30307263
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5810)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5810)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 5810)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350581.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350581.2
            Genome Coverage       :: 151x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:21:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,652
            CDSs (total)                      :: 5,557
            Genes (coding)                    :: 5,320
            CDSs (with protein)               :: 5,320
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 237
            CDSs (without protein)            :: 237
            Pseudo Genes (ambiguous residues) :: 0 of 237
            Pseudo Genes (frameshifted)       :: 102 of 237
            Pseudo Genes (incomplete)         :: 130 of 237
            Pseudo Genes (internal stop)      :: 52 of 237
            Pseudo Genes (multiple problems)  :: 41 of 237
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5810
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267800-rid25106413.denovo.266"
                     /serotype="O 26:H11"
                     /isolate="2201S31241"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-01"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            149..796
                     /gene="fucA"
                     /locus_tag="ACHOSC_RS20535"
                     /old_locus_tag="pgaptmp_004107"
     CDS             149..796
                     /gene="fucA"
                     /locus_tag="ACHOSC_RS20535"
                     /old_locus_tag="pgaptmp_004107"
                     /EC_number="4.1.2.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0008738 - L-fuculose-phosphate aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0006004 - fucose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-fuculose-phosphate aldolase"
                     /protein_id="HGU4549933.1"
                     /translation="MERNKLARQIIDTCLEMTRLGLNQGTAGNVSVRYQDGMLITPTG
                     IPYEKLTESHIVFIDGNGKHEEGKLPSSEWRFHMAAYQSRPDANAVVHNHAVHCTAVS
                     ILNRSIPAIHYMIAAAGGNSIPCAPYATFGTRELSEHVAQALKNRKATLLQHHGLIAC
                     EVNLEKALWLAHEVEVLAQLYLTTLAITDPVPVLSDEEIAVVLEKFKTYGLRIEE"
     gene            824..1972
                     /gene="fucO"
                     /locus_tag="ACHOSC_RS20540"
                     /old_locus_tag="pgaptmp_004108"
     CDS             824..1972
                     /gene="fucO"
                     /locus_tag="ACHOSC_RS20540"
                     /old_locus_tag="pgaptmp_004108"
                     /EC_number="1.1.1.77"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417279.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lactaldehyde reductase"
                     /protein_id="HGU4549934.1"
                     /translation="MANRMILNETAWFGRGAVGALTDEVKRRGYQKALIVTDKTLVQC
                     GVVAKVTDKMDAAGLAWAIYDGVVPNPTITVVKEGLGVFQNSGADYLIAIGGGSPQDT
                     CKAIGIISNNPEFADVRSLEGLSPTNKPSVPILAIPTTAGTAAEVTINYVITDEEKRR
                     KFVCVDPHDIPQVAFIDADMMDGMPPALKAATGVDALTHAIEGYITRGAWALTDALHI
                     KAIEIIAGALRGSVAGDKDAGEEMALGQYVAGMGFSNVGLGLVHGMAHPLGAFYNTPH
                     GVANAILLPHVMRYNADFTGEKYRDIARVMGVKVEGMSLEEARNAAVEAVFALNRDVG
                     IPPHLRDVGVRKEDIPALAQAALDDVCTGGNPREATLEDIVELYHTAW"
     gene            complement(2027..2782)
                     /gene="xni"
                     /locus_tag="ACHOSC_RS20545"
                     /old_locus_tag="pgaptmp_004109"
     CDS             complement(2027..2782)
                     /gene="xni"
                     /locus_tag="ACHOSC_RS20545"
                     /old_locus_tag="pgaptmp_004109"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417278.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0017108 - 5'-flap endonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0033567 - DNA replication, Okazaki fragment
                     processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flap endonuclease Xni"
                     /protein_id="HGU4549935.1"
                     /translation="MAVHLLIVDALNLIRRIHAVQGSPCVETCQHALDQLIMHSQPTH
                     AVAVFDDENRSSGWRHQRLPDYKAGRPPMPEELHDEMPALRAAFEQRGVPCWSTSGNE
                     ADDLAATLAVKVTQAGHQATIVSTDKGYCQLLSPTLRIRDYFQKRWLDAPFIDKEFGV
                     QPQQLPDYWGLAGISSSKVPGVAGIGPKSATQLLVEFQSLEGIYENLDAVAEKWRKKL
                     ETHKEMAFLCRDIARLQTDLHIDGNLQQLRLVR"
     gene            complement(2894..4261)
                     /gene="sdaB"
                     /locus_tag="ACHOSC_RS20550"
                     /old_locus_tag="pgaptmp_004110"
     CDS             complement(2894..4261)
                     /gene="sdaB"
                     /locus_tag="ACHOSC_RS20550"
                     /old_locus_tag="pgaptmp_004110"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase II"
                     /protein_id="HGU4549936.1"
                     /translation="MISVFDIFKIGIGPSSSHTVGPMKAGKQFTDDLIARNLLKDVTR
                     VVVDVYGSLSLTGKGHHTDIAIIMGLAGNLPDTVDIDSIPGFIQDVNTHGRLMLANGQ
                     HEVEFPVDQCMNFHADNLSLHENGMRITALAGDKVVYSQTYYSIGGGFIVDEEHFGQQ
                     NSAPVEVPYPYSSAADLQKHCQETGLSLSGLMMKNELALHSKEELEQHLANVWEVMRG
                     GIERGISTEGVLPGKLRVPRRAAALRRMLVSQDKTTTDPMAVVDWINMFALAVNEENA
                     AGGRVVTAPTNGACGIIPAVLAYYDKFIREVNANSLARYLLVASAIGSLYKMNASISG
                     AEVGCQGEVGVACSMAAAGLAELLGASPAQVCIAAEIAMEHNLGLTCDPVAGQVQVPC
                     IERNAIAAVKAVNAARMALRRTSEPRVCLDKVIETMYETGKDMNAKYRETSRGGLAMK
                     IVACD"
     gene            complement(4319..5608)
                     /gene="sdaC"
                     /locus_tag="ACHOSC_RS20555"
                     /old_locus_tag="pgaptmp_004111"
     CDS             complement(4319..5608)
                     /gene="sdaC"
                     /locus_tag="ACHOSC_RS20555"
                     /old_locus_tag="pgaptmp_004111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAAAP family serine/threonine permease SdaC"
                     /protein_id="HGU4549937.1"
                     /translation="METTQTSTIASKDSRSAWRKTDTMWMLGLYGTAIGAGVLFLPIN
                     AGVGGMIPLIIMAILAFPMTFFAHRGLTRFVLSGKNPGEDITEVVEEHFGIGAGKLIT
                     LLYFFAIYPILLVYSVAITNTVESFMSHQLGMTPPPRAILSLILIVGMMTIVRFGEQM
                     IVKAMSILVFPFVGVLMLLALYLIPQWNGAALETLSLDTASATGNGLWMTLWLAIPVM
                     VFSFNHSPIISSFAVAKREEYGDMAEQKCSKILAFAHIMMVLTVMFFVFSCVLSLTPA
                     DLAAAKEQNISILSYLANHFNAPVIAWMAPIIAIIAITKSFLGHYLGAREGFNGMVIK
                     SLRGKGKSIEINKLNRITALFMLVTTWIVATLNPSILGMIETLGGPIIAMILFLMPMY
                     AIQKVPAMRKYSGHISNVFVVVMGLIAISAIFYSLFS"
BASE COUNT         1441 a         1454 c         1570 g         1345 t
ORIGIN      
        1 gtgattagtt gaaccaggtc acaaaatata agcttgtgaa tttctttctg gttaatcagg
       61 aagaagatgg tgggtaatta aacggctaat tcaatagtgt gaaaggaaca acattattgc
      121 cctgttttga atcagagaga ggtagctaat ggaacgaaat aaacttgctc gtcagattat
      181 tgacacttgc ctggaaatga cccgcctggg actgaaccag gggacagcgg ggaacgtcag
      241 tgtacgttat caggatggga tgctgattac gcctacaggc attccatatg aaaaactgac
      301 ggagtcgcat attgtcttta ttgatggcaa cggtaaacat gaggaaggaa agctcccctc
      361 aagcgaatgg cgtttccata tggcggccta tcaaagcaga ccggatgcca acgcggttgt
      421 tcacaatcat gccgttcatt gcacggcagt ttccattctt aaccgatcga tccccgctat
      481 tcactacatg attgcggcgg ctggcggtaa ttctattcct tgcgcgcctt atgcgacctt
      541 tggaacacgt gaactttctg aacatgttgc gcaggctctc aaaaatcgta aggcaacttt
      601 gttacaacat catgggctta tcgcttgtga ggtgaatctg gaaaaagcgt tatggctggc
      661 gcatgaagtt gaagtgctgg cgcaacttta cctgacgacc ctggcgatta cggacccggt
      721 gccagtgctg agcgatgaag agattgccgt agtgctggag aaattcaaaa cctatgggtt
      781 acgaattgaa gagtaatttc gtaaagcaac aaggagaagg atgatggcta acagaatgat
      841 tctgaacgaa acggcatggt ttggtcgggg tgctgttggg gctttaaccg atgaggtgaa
      901 acgccgtggt tatcagaagg cgctgatcgt caccgataaa acgctggtgc aatgcggcgt
      961 ggtggcgaaa gtgaccgata agatggatgc tgcagggctg gcatgggcga tttacgacgg
     1021 cgtagtgccc aacccaacaa ttactgtcgt caaagaaggg ctcggtgtat tccagaatag
     1081 cggcgcggat tacctgatcg ctattggtgg tggttctcca caggatactt gtaaagcgat
     1141 tggcattatc agcaacaacc cggagtttgc cgatgtgcgt agcctggaag gactttcccc
     1201 gaccaataaa cccagtgtac cgattctggc aatccccacc acagcaggca ctgcggcaga
     1261 agtgaccatt aactacgtga tcactgacga agaaaaacgg cgcaagtttg tttgcgttga
     1321 tccgcatgat atcccgcagg tggcgtttat tgacgctgac atgatggatg gtatgcctcc
     1381 agcgctgaaa gctgcgacgg gtgtcgatgc gctcactcat gctattgagg ggtatattac
     1441 ccgtggcgcg tgggcgctaa ccgatgcact gcacattaaa gcgattgaaa tcattgctgg
     1501 ggcgctgcga ggatcggttg ctggtgataa ggatgccgga gaagaaatgg cgctcgggca
     1561 gtatgttgcg ggtatgggct tctcgaatgt tgggttaggg ttggtgcatg gtatggcgca
     1621 tccactgggc gcgttttata acactccaca cggtgttgca aacgccatcc tgctaccgca
     1681 tgtcatgcgc tataacgctg actttaccgg tgagaagtac cgcgatatcg cgcgcgttat
     1741 gggcgtgaaa gtggaaggta tgagcctgga agaggcgcgt aatgccgctg ttgaagcggt
     1801 gtttgctctc aaccgtgatg tcggtattcc gccacatttg cgtgatgttg gggtacgcaa
     1861 ggaagacatt ccggcactgg cgcaggcggc actggatgat gtttgtaccg gtggcaaccc
     1921 gcgtgaagca acgcttgagg atattgtaga gctttaccat accgcctggt aaatgcgctg
     1981 atgtgataat gccggatacg acgtttgcgg cgtatccggc tcgccgttac cgtaccaacc
     2041 gcaattgctg taaattgccg tcgatatgca aatcggtttg taagcgggca atatcgcggc
     2101 acagaaacgc catctcttta tgggtttcta attttttgcg ccacttttcg gcaaccgcat
     2161 ccagattctc atatatccct tccagactct gaaactcgac cagcagctgc gtggcgcttt
     2221 ttggtccgat tcccgcaaca cccggtacct ttgaactgct gatccccgca agtccccagt
     2281 aatcgggcaa ctgctgcggt tgaacgccaa attctttatc gataaatggc gcatccagcc
     2341 aacgtttctg gaagtaatca cgaatacgta atgtcggtga aagtaactga cagtagcctt
     2401 tatctgtcga aacaatcgtt gcctgatgcc cggcctgtgt cactttgacc gccagcgtgg
     2461 cggctaagtc atcggcttcg ttgccgctgg ttgaccagca cgggacgccg cgttgctcaa
     2521 aggcggcgcg taatgcaggc atctcgtcgt gcaactcttc cggcattggc ggtcgacccg
     2581 ctttgtaatc tggtaaacgc tgatgacgcc agccgctact gcggttttca tcatcaaaaa
     2641 cggcgaccgc gtgggttggc tggctgtgca taatgagctg atcgagcgca tgctggcagg
     2701 tttcgacaca gggcgatccc tgaacggcat gaatgcgacg aataagattc agtgcatcga
     2761 caataagcaa atgaacagcc acgataaccc ccttaaagat caggcgataa gggtaacatt
     2821 cgatgccgga acaggctacg gctggatgag aaatcgggaa gaggcctcgc aaaacgaggc
     2881 ctctggagag cgattaatcg caggcaacga tcttcattgc caggccgccg cgagaggttt
     2941 cgcggtactt ggcgttcata tctttacctg tttcgtacat ggtttcgata actttatcga
     3001 ggcagacgcg cggctcgctg gtacggcgta gcgccatacg tgcggcgttc accgctttta
     3061 ctgccgcaat ggcgttacgc tcgatgcatg gcacctggac ctgtccggcg accgggtcac
     3121 acgtcagacc gaggttgtgc tccatggcga tttccgccgc gatgcacacc tgcgccgggc
     3181 ttgcacctaa cagttctgcc agaccagccg ccgccattga gcacgccaca ccaacttcac
     3241 cctggcagcc gacttcagca ccagaaattg acgcgttcat cttataaaga gaaccaatgg
     3301 cactggctac cagcaggtaa cgagccagtg agttagcgtt cacttcgcgg ataaacttgt
     3361 cgtagtacgc cagcactgcc gggataatcc cgcacgcacc gttagtcggc gcagtcacca
     3421 cgcgtccgcc agcagcgttc tcttcgttca ctgccaatgc aaacatgttg atccagtcaa
     3481 caaccgccat cgggtcagtg gtggttttat cctggctgac cagcatccgg cgtagtgccg
     3541 cagcacggcg tggaacgcgc agtttgccag gcaacacgcc ttcggtggaa ataccgcgct
     3601 caataccgcc acgcatgact tcccatacgt tcgccaggtg ctgttccagc tcttctttgc
     3661 tgtgcagcgc cagctcattt ttcatcatca ggccagagag tgacagcccg gtttcctgac
     3721 aatgtttttg cagatcggct gctgaactgt acggataagg aacttcaacc ggtgcgctat
     3781 tctgctggcc aaaatgctct tcatcaacga taaagccacc gccaatagag tagtaagtct
     3841 ggctgtaaac gactttatcg cccgccagcg cggtaatgcg cataccgttt tcatgcagag
     3901 aaaggttgtc tgcatggaag ttcatgcact gatcaaccgg gaactccact tcatgctgac
     3961 cgtttgccag catcaggcga ccatgagtat tcacatcctg aataaaacca gggatggaat
     4021 cgatatccac ggtatccggc aggttacccg ccaggcccat aataatggcg atatcagtgt
     4081 ggtggccttt accagtcaga gagagcgagc cgtacacgtc aaccaccacg cgggtcacgt
     4141 ctttaagcag gttacgggca atcagatcgt cggtaaattg tttacccgct ttcattggtc
     4201 caacggtatg agaactggaa gggccaatgc cgattttgaa aatatcgaat acgctaatca
     4261 taggaaatac atcgcgttaa aacggaggaa gcgccgcccg aaagcggcgc gaaaggactt
     4321 agctgaacag agagtagaag attgcggaga ttgcaatcag acccatcacg acaacgaata
     4381 cgttgctgat gtgaccgctg tacttacgca ttgccggtac tttctgaatt gcgtacatcg
     4441 gcatcaggaa caggatcatc gcgatgattg ggccgcccag ggtttcaatc atacccagga
     4501 tgctcgggtt cagggtggca acaatccagg tcgttaccag catgaacagc gcagtgatac
     4561 ggttcagctt gttgatttcg atagacttac ctttaccacg cagagattta atcaccatac
     4621 cgttgaagcc ttcacgtgcg cccaggtagt ggccgaggaa ggatttggtg atagcgataa
     4681 tcgcgataat cggagccatc cacgcgataa ccggtgcgtt aaagtggtta gctaggtaag
     4741 acagaatcga gatgttctgc tctttagccg cagccaggtc tgccggagtc aggctcagta
     4801 cgcagctgaa gacgaagaac attacggtca gcaccatcat gatgtgtgcg aatgccagga
     4861 tcttcgagca tttctgttct gccatatcgc cgtactcttc acgcttcgca acggcgaaag
     4921 aagagatgat cggagagtgg ttgaacgaga acaccattac cggaattgcc agccacaggg
     4981 tcatccacag accgtttccg gttgcagatg cagtgtccag agacagcgtt tccagtgctg
     5041 cgccgttcca ctgcgggatc aggtacagag ccagcagcat cagtacgcca acaaacggga
     5101 ataccagaat actcatcgct ttaacgatca tctgctcacc aaagcgaacg atggtcatca
     5161 tacccacgat caggatcagc gacagaatcg cacgcggcgg tggcgtcata cccagctggt
     5221 gagacatgaa gctttcaact gtattggtga ttgccacgct ataaaccagc aggatcgggt
     5281 agatagcgaa gaagtagagc agggtaatca gtttacctgc gccaatacca aagtgttctt
     5341 ctacaacctc ggtgatgtct tcgcccgggt ttttaccaga cagtacgaag cgagtcaggc
     5401 cgcggtgagc aaaaaacgtc atcgggaacg caaggatagc catgatgatc agcgggatca
     5461 taccgccaac accggcgttg attggcagga acagcacgcc cgcgccgatt gccgtgccgt
     5521 aaaggcccag catccacatg gtgtctgtct tgcgccaggc actacgagag tctttcgacg
     5581 caatcgtgct ggtttgagtc gtttccatct atttctcctg gaggaatgca aataattcag
     5641 cttttagcca gttcagcccg ggaaggtgcc tgaccgtaaa aatgaaatct gtctatcggc
     5701 gatggcttaa tcatttaccg ccgtaataat ttgagggcgg aaagatacac acaagttatg
     5761 tatctatcag tgatcttgat cccaaataca ataatctact tggaagatag
//
LOCUS       DAZKHF020000265         5780 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2201S31241
            SAMN43267800-rid25106413.denovo.267, whole genome shotgun sequence.
ACCESSION   DAZKHF020000265 DAZKHF020000000
VERSION     DAZKHF020000265.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267800
            Sequence Read Archive: SRR30307263
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5780)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5780)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 5780)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350581.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350581.2
            Genome Coverage       :: 151x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:21:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,652
            CDSs (total)                      :: 5,557
            Genes (coding)                    :: 5,320
            CDSs (with protein)               :: 5,320
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 237
            CDSs (without protein)            :: 237
            Pseudo Genes (ambiguous residues) :: 0 of 237
            Pseudo Genes (frameshifted)       :: 102 of 237
            Pseudo Genes (incomplete)         :: 130 of 237
            Pseudo Genes (internal stop)      :: 52 of 237
            Pseudo Genes (multiple problems)  :: 41 of 237
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5780
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267800-rid25106413.denovo.267"
                     /serotype="O 26:H11"
                     /isolate="2201S31241"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-01"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            151..1134
                     /locus_tag="ACHOSC_RS20560"
                     /old_locus_tag="pgaptmp_004112"
     CDS             151..1134
                     /locus_tag="ACHOSC_RS20560"
                     /old_locus_tag="pgaptmp_004112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005224897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autoinducer 2 ABC transporter substrate-binding
                     protein"
                     /protein_id="HGU4549938.1"
                     /translation="MNKRFVINMVSSLLLGAALISAPLQAAEKVVVNISKVDGMPWFN
                     RMGEGVVEAGNAFGVNASQVGPSSTDAPQQVKIIEDLIARKVNAITIVPNDANVLEPV
                     FKKARDAGIVVLTNESPGQPSANWDIEIIDNEKFAAEYVEHMAKRMGGKGGYVIYVGS
                     LTVPQHNLWADLLVKYQKEHYPDMHEVTRRMPVAESVDDSRRTTLDLMKTYPDLKAVV
                     SFGSNGPIGAGRAVKEKRAKNKVAVYGMMIPSQAASLIKSGDITEGITYDSASAGYAL
                     AAVASTLLKGEEIKPGLEMQNLGKADVDMDKRIIRFHKVLLVNKDNIDSLY"
     gene            1183..2667
                     /locus_tag="ACHOSC_RS20565"
                     /old_locus_tag="pgaptmp_004113"
     CDS             1183..2667
                     /locus_tag="ACHOSC_RS20565"
                     /old_locus_tag="pgaptmp_004113"
                     /EC_number="7.5.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308402.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter ATP-binding protein"
                     /protein_id="HGU4549939.1"
                     /translation="METFLSLRHINKTFHATRALRDVSLDFMSGEVHCLAGQNGCGKS
                     TLIKIMSGVYRPDEGAEITLGGKNWSKLTPAASVAQGIQVIYQDLSLFPNLSVWENIA
                     VNHYHHGLFVNRRRLREVAQAAMTSINVTLPLDTLVSELSIARCQLVAICRALAQDAR
                     LIVMDEPTASLTHQEVQGLLQVVHQLRERGICVVFVSHRLEEVMEVSDRISVLKDGEL
                     VGTFPAAEMTTKQLGFLMTGQEFEYQVRELWQGKSSTPVLEVRNLSRHGEYLNINLRV
                     EAGEVVSIVGLLGAGRTELCLSLFGMTRPDAGEILINGQPVTLHSNQDAIRHGIGYVS
                     EDRMSRGLVMAQSIEDNIISTVFHKVKDRFGFLSETKVRDLVDRLIKALTIKVSDPHL
                     PVNTLSGGNAQRVSIAKWLAIGPRLLILDSPTVGVDIANKAGIYGIISDLAAHGIAVL
                     MICDEIEEAWYQSHRILVMQKGQITHSFLPDSSSQARIAEVVNG"
     gene            2660..3631
                     /locus_tag="ACHOSC_RS20570"
                     /old_locus_tag="pgaptmp_004114"
     CDS             2660..3631
                     /locus_tag="ACHOSC_RS20570"
                     /old_locus_tag="pgaptmp_004114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HGU4549940.1"
                     /translation="MAELKKRHEFWLALLIVVLFVGLAWRSDEFLTFGNLYDLANNYA
                     MLTILACGLFVVLISGGIDISFPAMTIIAQYGMVLLLQKIGGNFAVAFALAGCIGILL
                     GLINALLVNRLRVPSIIITISTLNIFYGLLLWLSKGVWLYDFPPWFEQGVMLFKYTDA
                     DGYDYGLGLPLIAMITVVLLTAFIMNFTSVGRKIYALGGNRESASRIGFSVLKLQLFV
                     YGYMGLMSGAAGVVQSWTVMTVAPDSLLGYELTVLAAVVLGGTSLLGGRGTLTGTLLG
                     VVLLAVMQNGLNLLGVSSYWQTLITGIIIVASISATAWSQHQNRSLL"
     gene            3628..4584
                     /locus_tag="ACHOSC_RS20575"
                     /old_locus_tag="pgaptmp_004115"
     CDS             3628..4584
                     /locus_tag="ACHOSC_RS20575"
                     /old_locus_tag="pgaptmp_004115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HGU4549941.1"
                     /translation="MKKSWRNNVEFYLIGLLVLTVAAFSITMPEIFWSISNFQSVASQ
                     MPVLGILALAMAVTMLCGGINLSIIATANACSLVMAWVATQYPPGIATVVATLLAGAG
                     AAVIIGLCNGVLIAGIRVSPILATLGMMTLLKGINILVTGGSAIANYPSWVLWLNHAQ
                     WFGIPLPMWLFAAVALGLWILLEKTPLGKAIYLIGSNERATLYSGINTRRVLIWVYVI
                     SALLCAVAAFLMMSKLNSAKASYGESYLLVSILAAVLGGVNPDGGSGRIIGMVLALFL
                     LQIIESGFNILGISPYLTMALWGTLLLCFIQVRGMLGLDRVV"
     gene            4671..5720
                     /gene="yahK"
                     /locus_tag="ACHOSC_RS20580"
                     /old_locus_tag="pgaptmp_004116"
     CDS             4671..5720
                     /gene="yahK"
                     /locus_tag="ACHOSC_RS20580"
                     /old_locus_tag="pgaptmp_004116"
                     /EC_number="1.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent aldehyde reductase YahK"
                     /protein_id="HGU4549942.1"
                     /translation="MKIKAVGAYSAKQPLEPMDITRREPGPNDVKIEIAYCGVCHSDL
                     HQVRSEWAGTVYPCVPGHEIVGRVVAVGDQVEKHAPGDLVGVGCIVDSCKHCEECEDG
                     LENYCDHMTGTYNSPTPDEPGHTLGGYSQQIVVHERYVLRIRHPQEQLAAVAPLLCAG
                     ITTYSPLRHWQAGPGKKVGVVGIGGLGHMGIKLAHAMGAHVVAFTTSEAKREAAKALG
                     ADEVVNSRNADEMAAHVKSFDFILNTVAAPHNLDDFTTLLKRDGTMTLVGAPATPHKS
                     PEVFNLIMKRRAIAGSMIGGIPETQEMLDFCAEHGIVADIEMIRADQINEAYERMLRG
                     DVKYRFVIDNRTLTD"
BASE COUNT         1314 a         1292 c         1622 g         1552 t
ORIGIN      
        1 cccgttttgt ccatatttat aaccatattt ttaatagctg cctgaaatag ggctgtttat
       61 tctgcataaa aatattcgaa aattcttatt ctctccagcg cgctaatttg ggcgaacact
      121 tcctgactac cctgcaatga ggctgaaatg atgaataaac gttttgttat caatatggtg
      181 tcttcgctgt tattaggcgc tgcgctaatt tctgcgccgc tccaggcagc ggaaaaagtc
      241 gtggtgaata tctctaaagt cgatggtatg ccgtggttta atcgcatggg cgaaggtgtg
      301 gttgaggcgg gtaacgcctt tggtgttaat gcgtcacagg tggggccttc aagtactgat
      361 gctccgcaac aagttaaaat tattgaggat ttaatcgccc gtaaggttaa tgcaattact
      421 atcgtgccaa acgatgccaa cgtgctggaa ccggtattca aaaaagcgcg agatgccgga
      481 attgtggttc tgaccaacga atcccccggt cagccaagtg ctaactggga tatcgaaatc
      541 attgataacg aaaaatttgc cgctgaatat gttgagcata tggcgaaacg catggggggc
      601 aagggcggct atgtgattta cgtgggtagt ttgaccgtgc cgcagcataa cttatgggct
      661 gacttgctgg tgaaatacca gaaagagcat tacccggata tgcatgaagt tacccgccgc
      721 atgccggttg ccgagagtgt ggatgactca cgtcgtacca cactggacct gatgaaaacc
      781 tatcctgact tgaaagcggt tgtctccttt ggttcaaacg gtccgattgg cgcgggacgt
      841 gcggtgaaag agaaacgggc gaaaaataaa gtcgcagtct atggcatgat gatcccttca
      901 caggcggcat cactgattaa aagtggtgat atcaccgaag ggattactta cgattcggcc
      961 tctgcaggtt acgcactcgc agccgtcgcc agcacgttgc tgaagggtga agagatcaaa
     1021 cctggattag aaatgcagaa tctgggtaag gccgacgtag atatggataa gcgaattatc
     1081 cgtttccata aggtattact ggtcaacaaa gacaatatcg actcacttta ttaatccatt
     1141 tcttcggggc gaaagccccg attcttattg ccaggggatg ttatggaaac cttcctttcc
     1201 cttcgtcata tcaacaaaac gtttcatgcc acgcgagcgc tgcgcgatgt ttctctcgac
     1261 tttatgtcgg gtgaagtgca ctgccttgcc gggcaaaacg gctgtggtaa atcgacgttg
     1321 attaaaatta tgtccggtgt ttatcgcccg gatgagggag cggaaattac gcttggtggg
     1381 aaaaactggt caaagctgac acccgccgct tcggtggcgc aggggattca ggtgatttat
     1441 caggacctct ctttatttcc taacctgagt gtctgggaga atatcgccgt gaatcactat
     1501 caccacggtc tgtttgttaa ccgccgtcgt ctgcgtgagg tggcgcaggc ggcaatgacc
     1561 agtattaacg tcacattgcc actggatacg ctcgtttctg agctttcaat tgcccgctgt
     1621 cagctggtag caatttgccg tgcactggcg caggacgcac ggttgatcgt gatggatgaa
     1681 ccaaccgctt cgctgaccca tcaggaagtg caagggttat tacaggtggt gcatcaattg
     1741 cgggaacgcg ggatctgcgt ggtcttcgtc agtcatcgtc tggaagaagt gatggaagtt
     1801 tccgaccgta tttcagtgct gaaagatggt gagctggtcg ggacttttcc ggctgcagag
     1861 atgaccacaa aacaactcgg tttcctgatg accggccagg agttcgaata tcaggtgcga
     1921 gagttgtggc agggaaaatc cagcaccccg gtgcttgagg tgcgtaattt aagtcgtcat
     1981 ggggaatatc taaatattaa cttgcgggtg gaagcgggcg aagtggtatc gattgttggc
     2041 ctgcttggtg cggggcgcac ggaattatgt ctgagtctgt ttggtatgac ccgaccggat
     2101 gctggcgaga tcctcatcaa cggccagccg gttacactgc atagtaacca ggacgctatc
     2161 cgccacggta ttggttatgt ttcggaagat cgcatgtcgc gcggtttggt gatggcgcaa
     2221 tctattgaag acaacatcat tagcactgtt tttcacaagg taaaagatcg cttcggcttt
     2281 ctgagtgaaa ctaaagttcg tgacctggtg gacaggctaa tcaaggcatt gaccatcaaa
     2341 gtctctgatc ctcatcttcc ggtgaatacg ctttctggtg gtaacgcgca gcgagtatcg
     2401 atcgccaaat ggctggcgat tgggccacga ctgttgattc tcgatagccc gacagtcggt
     2461 gttgatatcg ccaacaaagc aggaatatac ggcattatca gcgatctggc ggctcatggc
     2521 attgcggtat tgatgatctg cgatgaaatt gaagaagcgt ggtatcaaag ccaccggatt
     2581 ttggtaatgc aaaaaggtca gatcacccat agcttcctgc ctgacagcag ttctcaggcc
     2641 aggattgcgg aggtggtaaa tggctgaact gaaaaaacgc catgaattct ggctggcatt
     2701 actgattgtg gtgctgtttg ttggccttgc ctggcgcagc gacgagtttc tgacattcgg
     2761 taatttgtac gatctcgcca ataactatgc catgttgact attctcgcct gtggtttgtt
     2821 tgtggtgttg atttccggtg gaattgatat ttcgtttcca gcaatgacca tcattgcgca
     2881 atacggcatg gtgctgttgc tgcaaaaaat tggtggcaac ttcgctgtcg cgtttgcact
     2941 ggcgggctgc atcggcattt tacttggctt aattaacgcc ttactggtta atcgcctacg
     3001 ggtgccttct atcatcatca ctatctcgac gctgaatatt ttctatggcc tgctgttatg
     3061 gttgagtaaa ggtgtgtggc tgtacgactt tccgccgtgg tttgagcagg gggttatgtt
     3121 gttcaagtac accgatgctg atggctatga ctatggcctt ggtctgccgc tgatcgccat
     3181 gattacggtg gtgctgctga cagcgtttat catgaatttc accagtgtag ggcgcaaaat
     3241 ttatgccctt ggcgggaacc gcgaatcagc cagtcgcatc ggctttagcg tgctgaaact
     3301 gcaacttttc gtctacggct atatgggatt gatgtctggc gctgcgggtg tagtgcagtc
     3361 gtggacggtg atgactgtcg cccccgattc tcttctgggt tatgagctga cagtactggc
     3421 tgcggtggtg cttggcggca ctagtttgct cggcgggcgc ggcacgttaa ccggtacttt
     3481 gctcggcgtg gtgttgttgg cagtgatgca aaacgggcta aatttattgg gagtctcgtc
     3541 ttactggcaa acattgatca ccggcatcat catcgttgcc agcattagtg ccacggcgtg
     3601 gagtcagcat cagaaccgga gtctgctatg aaaaaatcgt ggcgtaataa cgttgagttt
     3661 tacttgatcg gcctgctggt gctaactgtt gccgcgttca gcattactat gccggagatt
     3721 ttctggtcga taagtaattt ccagtccgtc gcttcgcaaa tgcccgtgct gggcattctg
     3781 gcgttggcta tggcggtgac catgctttgc ggtggcatta acttgtcaat tatagccact
     3841 gctaacgcct gttcactggt gatggcgtgg gtggcgactc agtatccacc gggtattgct
     3901 acggttgtcg ctacgttact ggcgggcgct ggcgcggcgg tgataatcgg tttatgtaac
     3961 ggcgtattga ttgctggtat tcgagtgtcg ccaattcttg caacgcttgg gatgatgacg
     4021 ttgctcaaag ggataaatat tctggtgaca ggtggtagcg ccattgccaa ttatccctca
     4081 tgggtgctgt ggttgaatca tgcgcaatgg ttcggtattc cgctaccgat gtggttgttt
     4141 gccgcagtgg cactggggct gtggatattg ctggagaaaa cgccgctcgg taaagccatt
     4201 tacctgattg ggtctaacga acgagcaaca ctttactccg gcattaacac tcgtcgggtg
     4261 ctgatttggg tttatgtcat ttccgccttg ctttgtgcgg tagcggcatt tttgatgatg
     4321 tcgaaactca actcggctaa ggcctcttat ggcgaatcgt acttgctggt gtcaatcctc
     4381 gcggcagtac tcggcggggt taatccggac ggcggcagcg ggcggataat tggtatggtg
     4441 ctggcgctgt ttcttctgca aattattgaa agcggcttca atattctcgg tataagcccc
     4501 tacctgacga tggccttatg gggaacgttg ctgctctgtt tcattcaggt tcgcggcatg
     4561 ttggggctgg atcgggtggt ttaaatattg gctatattca atggacgcgt tttgccgcga
     4621 tgacatatca ggcatcgcca aatacacata gctaatcagg agtaaacaca atgaagatca
     4681 aagctgttgg tgcatattcc gctaaacaac cacttgaacc gatggatatc acacggcgtg
     4741 aaccgggacc gaatgatgtc aaaatcgaaa tcgcttactg tggcgtctgc cattccgatc
     4801 tccaccaggt ccgttccgag tgggcgggga cggtttaccc ctgcgtaccg ggtcatgaaa
     4861 ttgtggggcg tgtggtagcc gttggtgatc aggtagaaaa acatgcgccg ggcgatctgg
     4921 tcggtgtcgg ctgcattgtc gacagttgta aacattgcga agagtgtgaa gacgggctgg
     4981 aaaactactg tgatcacatg accggcacct ataactcgcc gacgccggac gaaccgggcc
     5041 atactctggg cggctactca caacagatcg tcgttcatga gcgatatgtt ctgcgtattc
     5101 gtcacccgca agagcagctg gcggcggtgg ctcctttgtt gtgtgcaggg atcaccacgt
     5161 attcgccgct acgtcactgg caggccgggc cgggtaaaaa agtgggcgtg gtcggcatcg
     5221 gcggcctggg acatatgggg attaagctgg cccacgcgat gggggcacat gtggtggcat
     5281 ttaccacttc tgaggcaaaa cgcgaagcgg caaaagccct gggggccgat gaagttgtta
     5341 actcacgcaa tgccgatgag atggcggctc atgtgaagag tttcgatttc attttgaata
     5401 cagtagctgc gccacataat ctcgacgatt ttaccacctt gctgaagcgt gatggcacca
     5461 tgacgctggt tggtgcgcct gcgacaccgc ataaatcgcc ggaagttttc aacctgatca
     5521 tgaaacgccg tgcgatagcc ggttctatga ttggcggcat tccagaaact caggagatgc
     5581 tcgatttttg cgccgaacat ggcatcgtgg ctgatataga gatgattcgg gccgatcaaa
     5641 ttaatgaagc ctatgagcga atgctgcgtg gtgatgtgaa atatcgtttt gttatcgata
     5701 atcgcacact aacagactga aaaaattaat aaatacgctg tggtttaaca tattaacttc
     5761 gctctccact taacttttta
//
LOCUS       DAZKHF020000266         5776 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2201S31241
            SAMN43267800-rid25106413.denovo.268, whole genome shotgun sequence.
ACCESSION   DAZKHF020000266 DAZKHF020000000
VERSION     DAZKHF020000266.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267800
            Sequence Read Archive: SRR30307263
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5776)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5776)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 5776)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350581.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350581.2
            Genome Coverage       :: 151x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:21:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,652
            CDSs (total)                      :: 5,557
            Genes (coding)                    :: 5,320
            CDSs (with protein)               :: 5,320
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 237
            CDSs (without protein)            :: 237
            Pseudo Genes (ambiguous residues) :: 0 of 237
            Pseudo Genes (frameshifted)       :: 102 of 237
            Pseudo Genes (incomplete)         :: 130 of 237
            Pseudo Genes (internal stop)      :: 52 of 237
            Pseudo Genes (multiple problems)  :: 41 of 237
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5776
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267800-rid25106413.denovo.268"
                     /serotype="O 26:H11"
                     /isolate="2201S31241"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-01"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            18..758
                     /gene="yiaT"
                     /locus_tag="ACHOSC_RS20585"
                     /old_locus_tag="pgaptmp_004117"
     CDS             18..758
                     /gene="yiaT"
                     /locus_tag="ACHOSC_RS20585"
                     /old_locus_tag="pgaptmp_004117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0030674 - protein-macromolecule adaptor
                     activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MipA/OmpV family protein"
                     /protein_id="HGU4549943.1"
                     /translation="MLINRNIVALFALPFMASATASELSIGAGAAYNESPYRGYNENT
                     KAIPLISYEGDSFYVRQTTLGFILSQSEKNELSLTASWMPLEFDPADNDDYAMQQLDK
                     RDSTAMAGVAWYHHERWGTVKASAAADVLDNSNGWVGELSVFHKMQIGRLSLTPALGV
                     LYYDENFSDYYYGISESESRRSGLASYSAQDAWVPYVSLTAKYPIGEHVVLMASAGYS
                     ELPEEITNSPMIDRNESFTFITGVSWRF"
     gene            complement(705..968)
                     /locus_tag="ACHOSC_RS20590"
                     /old_locus_tag="pgaptmp_004118"
                     /pseudo
     CDS             complement(705..968)
                     /locus_tag="ACHOSC_RS20590"
                     /old_locus_tag="pgaptmp_004118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020239594.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            972..1073
                     /locus_tag="ACHOSC_RS20595"
                     /old_locus_tag="pgaptmp_004119"
                     /pseudo
     CDS             972..1073
                     /locus_tag="ACHOSC_RS20595"
                     /old_locus_tag="pgaptmp_004119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312484.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
     gene            complement(1105..1800)
                     /gene="araD"
                     /locus_tag="ACHOSC_RS20600"
                     /old_locus_tag="pgaptmp_004120"
     CDS             complement(1105..1800)
                     /gene="araD"
                     /locus_tag="ACHOSC_RS20600"
                     /old_locus_tag="pgaptmp_004120"
                     /EC_number="5.1.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0008742 - L-ribulose-phosphate 4-epimerase
                     activity [Evidence IEA];
                     GO_process: GO:0019568 - arabinose catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 4-epimerase"
                     /protein_id="HGU4549944.1"
                     /translation="MLEQLKADVLAANLALPAHHLVTFTWGNVSAVDETRQWMVIKPS
                     GVEYDVMTADDMVVVEIASGKVVEGSKKPSSDTPTHLALYRRYAEIGGIVHTHSRHAT
                     IWSQAGLDLPAWGTTHADYFYGAIPCTRQMTAEEINGEYEYQTGEVIIETFEERGRSP
                     AQIPAVLVHSHGPFAWGKNAADAVHNAVVLEECAYMGLFSRQLAPQLPAMQNELLDKH
                     YLRKHGANAYYGQ"
     gene            complement(1794..2654)
                     /gene="sgbU"
                     /locus_tag="ACHOSC_RS20605"
                     /old_locus_tag="pgaptmp_004121"
     CDS             complement(1794..2654)
                     /gene="sgbU"
                     /locus_tag="ACHOSC_RS20605"
                     /old_locus_tag="pgaptmp_004121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418039.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016861 - intramolecular oxidoreductase
                     activity, interconverting aldoses and ketoses [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 3-epimerase"
                     /protein_id="HGU4549945.1"
                     /translation="MRNHPLGIYEKALAKDLSWPERLVLAKSCGFDFVEMSVDETDER
                     LSRLDWSTAQRTSLVAAMIETGVGIPSMCLSAHRRFPFGSRDDAVRQRAREIMSKAIR
                     LARDLGIRTIQLAGYDVYYEDHDEGTRQRFAEGLAWAVEQAAASQVMLAVEIMDTAFM
                     NSISKWKKWDEMLASPWFTVYPDVGNLSAWGNDVPAELKLGIDRIAAIHLKDTQPVTE
                     QSPGQFRDVPFGEGCVDFVGIFKTLHKLNYRGSFLIEMWTEKAKEPVLEIIQARRWIE
                     ARMQEAGFIC"
     gene            complement(2647..3309)
                     /gene="ulaD"
                     /locus_tag="ACHOSC_RS20610"
                     /old_locus_tag="pgaptmp_004122"
     CDS             complement(2647..3309)
                     /gene="ulaD"
                     /locus_tag="ACHOSC_RS20610"
                     /old_locus_tag="pgaptmp_004122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-keto-L-gulonate-6-phosphate decarboxylase
                     UlaD"
                     /protein_id="HGU4549946.1"
                     /translation="MSRPLLQLALDHSSLEAAQRDVTQLKDSVDIVEAGTILCLNEGL
                     GAVKALREQCPDKIIVADWKVADAGETLAQQAFGAGANWMTIICAAPLATVEKGHAMA
                     QRCGGEIQIELFGNWTLDDARDWHRIGVRQAIYHRGRDAQASGQQWGEADLARMKALS
                     DIGLELSITGGITPADLPLFKDIRVKAFIAGRALAGSANPAQVAGDFHAQIDAIWGGK
                     RA"
     gene            complement(3306..4802)
                     /gene="lyxK"
                     /locus_tag="ACHOSC_RS20615"
                     /old_locus_tag="pgaptmp_004123"
     CDS             complement(3306..4802)
                     /gene="lyxK"
                     /locus_tag="ACHOSC_RS20615"
                     /old_locus_tag="pgaptmp_004123"
                     /EC_number="2.7.1.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-xylulokinase"
                     /protein_id="HGU4549947.1"
                     /translation="MTQYWLGLDCGGSWLKAGLYDREGREAGVQRLPLCALSPQPGWA
                     ERDMAELWQCCMAVIRELLTHSGVSGEQIVGIGISAQGKGLFLLDKNNKPLGNAILSS
                     DRRAMEIVRRWQEDGIPEKLYPLTRQTLWTGHPVSLLRWLKEHEPERYAQIGCVMMTH
                     DYLRWCLTGVKGCEESNISESNLYNMSLGEYDPCLTDWLGIAEINHTLPPVVGSAEIC
                     GEITAQTAVLTGLKAGTPVVGGLFDVVSTALCAGIEDEFTLNAVMGTWAVTSGITRGL
                     RDGEAHPYVYGRYVNDGEFIVHEASPTSSGNLEWFTAQWGEISFDEINQAVASLPKAG
                     GDLFFLPFLYGSNAGLEMTSGFYGMQAIHTRAHLLQAIYEGVVFSHMTHLNRMRERFT
                     DVHTLRVTGGPAHSDVWMQMLADVSGLRIELPQVEETGCFGAALAARVGTGVYRDFSE
                     AQRDLQHPVRTLLPDMTVHQLYQQKYQRYQHLIAALQGFHARIKEHIL"
     gene            complement(4806..>5776)
                     /gene="yiaO"
                     /locus_tag="ACHOSC_RS20620"
                     /old_locus_tag="pgaptmp_004124"
     CDS             complement(4806..>5776)
                     /gene="yiaO"
                     /locus_tag="ACHOSC_RS20620"
                     /old_locus_tag="pgaptmp_004124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="2,3-diketo-L-gulonate TRAP transporter
                     substrate-binding protein YiaO"
                     /protein_id="HGU4549948.1"
                     /translation="TYALFIAGLAAFSTSSLAAQSLRFGYETSQTDSQHIAAKKFNDL
                     LQERTKGELKLKLFPDSTLGNAQAMISGVRGGTIDMEMSGSNNFAGLSPVMNLLDVPF
                     LFRDTAHAHKTLDGKVGDDLKASLEGKGLKVLAYWENGWRDVTNSRAPVKTPADLKGL
                     KIRTNNSPMNIAAFKVFGANPIPMPFAEVYTGLETRTIDAQEHPINVVWSAKFFEVQK
                     YLSLTHHAYSPLLVVINKAKFDGLSPEFQQALISSAQEAGNYQRKLVAEDQQKIIDGM
                     KEAGVEVITDLDRKAFSDALGTQVRDMFVKDVPQGADLLKAVDEVQ"
BASE COUNT         1240 a         1586 c         1587 g         1363 t
ORIGIN      
        1 aaaatggaat accggacatg ttaattaatc gcaatattgt ggcgttattt gcgttgcctt
       61 ttatggcaag cgcaactgct tctgaattat ccattggtgc tggtgcagct tataatgaat
      121 cgccttatcg cggttataat gaaaatacaa aggcaattcc gctgattagt tatgaaggtg
      181 attcttttta tgttcgtcag accacattag gttttattct gtcgcaaagt gaaaaaaatg
      241 aacttagcct gaccgcatcc tggatgccgc tggaatttga ccctgccgat aatgacgatt
      301 atgccatgca acagcttgat aagcgtgata gtacggctat ggcgggggtt gcctggtatc
      361 accacgagcg ttggggaacc gtgaaagcct ctgcggctgc ggacgttctg gataacagca
      421 acggctgggt gggggagcta tcggtattcc acaaaatgca gataggtcgc ctgtcgctga
      481 cacctgcgct gggcgttctc tattatgacg agaatttcag tgactattac tatggcattt
      541 cagagagtga gtcccgtcgt agcggtctgg caagttattc cgcgcaggat gcctgggtgc
      601 cctatgtcag cctgacggca aaatacccga taggagagca cgtcgtattg atggcgagcg
      661 caggatacag cgagctgccg gaagagatta ccaacagccc gatgattgat cgtaatgaga
      721 gttttacctt tatcaccggc gtgagctggc gtttttaatt caccggtgga tgtcggtgcg
      781 gctggtgctc cgtcgtaatg ccacaaaggt tgttgctctg gattgctctg ttcataccgg
      841 gaacctggcg tctgaatcag gatcttatgg ctgaacaatc ccttgccttc aaatgtcgta
      901 aatggcgcat cggcgggcag caacaggtta tgcagtgact cgacgttgtc ggcctgctcc
      961 atgcaatacg gtttaatgac gttggctatt tccggcagat ttttcgcaag cacaccggtt
     1021 taacgccagc agcgtggaag cggcgttaca gcaaggaaca tatcaattcg tagtgccggg
     1081 gcgatgaagc cccggcgtga gggattactg cccgtaatag gcattggccc catgcttacg
     1141 caggtagtgc ttatccagca gttcgttttg catcgcaggg agctgcggcg caagctggcg
     1201 cgagaatagg cccatatagg cgcattcttc gagtactacg gcgttatgca cggcatcggc
     1261 ggcgttttta ccccatgcga acgggccgtg agaatgcacc agcaccgccg ggatttgtgc
     1321 cggactcctg ccacgttctt cgaaggtttc aatgatcact tcgccggtct gatattcata
     1381 ttcgccgtta atctcctctg cggtcatctg tcgcgtgcag gggatggcac catagaaata
     1441 atcagcgtgc gtggtgcccc aggcggggag atccagcccg gcctgcgacc agatggtggc
     1501 gtggcgtgag tgggtatgca caatgccacc gatttcggcg tagcgacggt aaagcgccag
     1561 atgcgttggc gtgtcggacg agggcttttt gctaccttcc accaccttgc cgctggctat
     1621 ctcaaccacc accatatcgt cggcggtcat cacgtcatac tcgacgccgg aaggtttgat
     1681 taccatccat tgccgcgttt cgtctaccgc gctgacattg ccccaggtga acgtcaccag
     1741 atggtgagcg ggaagcgcca gattcgccgc cagcacgtcg gctttcagtt gctctaacat
     1801 ataaatccag cctcctgcat acgcgcttca atccaacgcc gcgcctgaat aatctccagc
     1861 accggctctt tggctttttc ggtccacatc tcaatcagga aagaaccgcg atagttcagt
     1921 ttatgcagcg ttttgaagat gccaacgaaa tcgacgcagc cttcgccaaa cggcacgtcg
     1981 cggaactgtc cgggactttg ctcggtaact ggctgggtgt ctttcaggtg gatcgcggcg
     2041 atacggtcaa tgcccagttt cagttcggcg ggaacatcat tgccccaggc gctgaggttg
     2101 ccgacgtccg ggtagacggt gaaccacggc gaggcgagca tctcgtccca ctttttccat
     2161 ttactgatgg agttcataaa cgcggtgtcc ataatctcca ccgctagcat gacttgtgat
     2221 gccgccgcct gttcgaccgc ccacgccagc ccttcagcaa aacgttgccg ggtgccttca
     2281 tcgtggtctt cgtaatagac gtcgtagccc gccagctgga tggtgcgtat gccgagatcg
     2341 cgtgccagac gaatcgcttt gctcatgatc tcccgtgctc gctggcgtac cgcgtcgtca
     2401 cggctaccaa aaggaaagcg acgatgtgcg gacaggcaca tcgacgggat cccaacaccc
     2461 gtttcgatca tcgcggcaac cagcgaagtc ctttgtgcgg tgctccagtc aagacgtgaa
     2521 agccgttcgt cggtttcatc caccgacatt tcgacaaaat caaaaccgca gctttttgcc
     2581 agcaccagcc gctccggcca ggagagatct ttcgccagcg ccttttcata aatccctaac
     2641 ggatgattac gcacgcttgc ctccccagat agcgtcgatt tgcgcatgga aatcgccagc
     2701 cacctgcgcc ggatttgctg agcctgccag tgctcgcccg gcaataaacg ctttcacgcg
     2761 aatatcttta aacagcggca ggtcagcagg ggtaatcccg ccggtaatgg aaagctcaag
     2821 gccgatatcg gaaagcgcct tcatgcgtgc cagatcggct tcgccccact gttgcccgct
     2881 ggcctgtgca tcacgaccgc gatgataaat cgcctgccgc acgccaatac gatgccagtc
     2941 gcgggcgtcg tccagcgtcc agttaccgaa cagctcgatc tgaatttcac caccgcagcg
     3001 ttgcgccatc gcgtggcctt tttccaccgt cgcgagcggt gcggcgcaga tgatggtcat
     3061 ccagttagcg cctgcgccaa atgcctgttg cgcgagcgtt tcaccagcgt cggcgacctt
     3121 ccagtcggca acgatgattt tgtcagggca ctgttcgcgc aaggctttca ccgcgccaag
     3181 cccttcgttt aaacagagaa tggtgcccgc ttcgacaata tcgacgctgt cttttaactg
     3241 cgtcacgtcg cgctgtgcgg cttcaagtga tgagtggtcg agtgccagtt gcagaagtgg
     3301 tcggctcata atatgtgctc cttgatgcgg gcgtgaaagc cctgaagtgc ggcaatgaga
     3361 tgctgatagc gttggtattt ttgttggtaa agctgatgga cagtcatatc cggcagcagg
     3421 gtgcgcaccg ggtgctgtaa atcacgctgg gcttcgctga aatcgcgata aaccccggtg
     3481 ccgacgcggg cggcaagtgc tgcaccaaag cagccggttt cttccacctg cggcagctca
     3541 atacgcagac cgctgacgtc cgccagcatt tgcatccaga catcggaatg cgccgggccg
     3601 ccagtgacgc gcagggtgtg cacatcagta aaacgttcgc gcatccggtt gagatgggtc
     3661 atatggctga acaccacccc ttcataaatg gcctgcaaca ggtgtgcgcg ggtgtgaatg
     3721 gcctgcatcc cgtagaaacc gctggtcatc tcgagtccgg cgttgctgcc gtacaggaac
     3781 ggcaggaaaa agagatcgcc accggccttc ggcaagctgg caacggcctg gttgatctca
     3841 tcaaacgaga tttctcccca ctgtgcggta aaccattcga ggttgccgga agaggtgggg
     3901 ctggcttcgt gaacgataaa ttcaccatcg ttaacgtagc gaccatagac atacggatgc
     3961 gcttcaccgt cacgtaaacc gcgggttatg ccgctggtca ccgcccaggt gcccatcacc
     4021 gcattaaggg taaattcgtc ttcgatcccg gcgcagagtg cggtggaaac cacatcaaac
     4081 aggccgccga caacgggcgt acccgctttc agaccggtca gtacggctgt ctgagcggtg
     4141 atctccccgc agatttcggc agatccgaca acaggcggca gggtgtgatt gatttcagca
     4201 atccccagcc agtcggtgag gcacgggtca tattccccaa gactcatgtt gtagagattg
     4261 gactcggaaa tattgctctc ttcacagcct ttgacgccgg ttaaacacca gcgcaggtag
     4321 tcgtgcgtca tcatcacgca gccaatttgc gcgtagcgtt ccggttcgtg ctctttaagc
     4381 caacgtaaca gcgataccgg atgcccggtc cacaaggttt gtcgggtcag cgggtagagt
     4441 ttttccggga tgccatcttc ctgccagcga cgaacgattt ccatcgcccg gcgatccgag
     4501 gataaaatgg cgttaccgag cggcttgttg tttttatcca gcaaaaacaa gccttttcct
     4561 tgtgcggaga taccgatacc gacaatttgt tccccgctaa cgccggaatg ggtaagcagt
     4621 tcgcgaatga cagccatgca gcattgccac agttctgcca tatcgcgctc tgcccaacct
     4681 ggctgcgggc ttaatgcgca cagcggcagg cgctgcacgc ctgcctcccg gccttcgcgg
     4741 tcatacagcc cggctttcag ccagctaccg ccacaatcta accccagcca gtattgcgtc
     4801 atggtttatt gcacctcatc cacggctttc agtagatcag caccctgcgg aacatctttt
     4861 acaaacatgt cgcggacctg agtacccagt gcgtcgctaa aggctttgcg gtcgagatcg
     4921 gtgatgactt ccacgcccgc ttctttcatg ccgtcgatga ttttttgctg atcttcggca
     4981 accagtttgc gctggtagtt acccgcttcc tgcgcagatg aaattagcgc ttgctggaac
     5041 tccgggctta agccatcaaa cttcgctttg ttgatcacca ccagaagcgg ggaataggcg
     5101 tggtgcgtca gagaaaggta cttctgcact tcgaaaaatt ttgctgacca gacgacgttg
     5161 atcgggtgtt cctgagcgtc gatagtgcgg gtttccagcc cggtatagac ttcggcaaac
     5221 ggcatcggga tcgggttagc gccaaagact ttgaatgcgg cgatattcat cgggctattg
     5281 ttggtgcgaa ttttcagccc tttcaggtcg gcgggggttt taaccggtgc gcgcgagttg
     5341 gtgacatcgc gccagccgtt ttcccagtag gccagtactt ttagtccttt accttcaagt
     5401 gaggctttca gatcatcacc gactttgccg tcgagcgttt tatgcgcgtg agcggtatcg
     5461 cggaacagga aagggacatc aagcaagttc atcactggtg ataatccggc aaagttattc
     5521 gagccggaca tttccatatc gatggtgccg ccacgtacgc cgctgatcat cgcctgcgcg
     5581 ttaccgaggg tgctatccgg aaacaatttt aatttcagct cgcctttggt tctctcctgc
     5641 aataaatcat tgaatttttt cgccgcaata tgttgcgagt cggtttgtga ggtttcataa
     5701 ccgaaacgta aagattgcgc cgccagagaa gatgtgctga atgcagccag gccagcaatg
     5761 aataatgcgt aggtta
//
LOCUS       DAZKHF020000267         5753 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2201S31241
            SAMN43267800-rid25106413.denovo.269, whole genome shotgun sequence.
ACCESSION   DAZKHF020000267 DAZKHF020000000
VERSION     DAZKHF020000267.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267800
            Sequence Read Archive: SRR30307263
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5753)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5753)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 5753)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350581.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350581.2
            Genome Coverage       :: 151x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:21:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,652
            CDSs (total)                      :: 5,557
            Genes (coding)                    :: 5,320
            CDSs (with protein)               :: 5,320
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 237
            CDSs (without protein)            :: 237
            Pseudo Genes (ambiguous residues) :: 0 of 237
            Pseudo Genes (frameshifted)       :: 102 of 237
            Pseudo Genes (incomplete)         :: 130 of 237
            Pseudo Genes (internal stop)      :: 52 of 237
            Pseudo Genes (multiple problems)  :: 41 of 237
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5753
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267800-rid25106413.denovo.269"
                     /serotype="O 26:H11"
                     /isolate="2201S31241"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-01"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            complement(306..1265)
                     /gene="nrdF"
                     /locus_tag="ACHOSC_RS20625"
                     /old_locus_tag="pgaptmp_004125"
     CDS             complement(306..1265)
                     /gene="nrdF"
                     /locus_tag="ACHOSC_RS20625"
                     /old_locus_tag="pgaptmp_004125"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005971 - ribonucleoside-diphosphate
                     reductase complex [Evidence IEA];
                     GO_function: GO:0004748 - ribonucleoside-diphosphate
                     reductase activity, thioredoxin disulfide as acceptor
                     [Evidence IEA];
                     GO_process: GO:0009263 - deoxyribonucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class 1b ribonucleoside-diphosphate reductase
                     subunit beta"
                     /protein_id="HGU4549949.1"
                     /translation="MKLSRISAINWNKISDDKDLEVWNRLTSNFWLPEKVPLSNDIPA
                     WQTLSVVEQQLTMRVFTGLTLLDTLQNVIGAPSLMPDALTPHEEAVLSNISFMEAVHA
                     RSYSSIFSTLCQTKDVDAAYAWSEENAPLQRKAQIIQQHYRGDDPLKKKIASVFLESF
                     LFYSGFWLPMYFSSRGKLTNTADLIRLIIRDEAVHGYYIGYKYQKNMEKISLGQREEL
                     KSFAFDLLLELYDNELQYTDELYAETPWADDVKAFLCYNANKALMNLGYEPLFPAEMA
                     EVNPAILAALSPNADENHDFFSGSGSSYVMGKAVETEDEDWNF"
     gene            complement(1275..3419)
                     /gene="nrdE"
                     /locus_tag="ACHOSC_RS20630"
                     /old_locus_tag="pgaptmp_004126"
     CDS             complement(1275..3419)
                     /gene="nrdE"
                     /locus_tag="ACHOSC_RS20630"
                     /old_locus_tag="pgaptmp_004126"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004748 - ribonucleoside-diphosphate
                     reductase activity, thioredoxin disulfide as acceptor
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class 1b ribonucleoside-diphosphate reductase
                     subunit alpha"
                     /protein_id="HGU4549950.1"
                     /translation="MATTTAECLTQETMDYHALNAMLNLYDSAGRIQFDKDRQAVDAF
                     IATHVRPNSVTFSSQQQRLNWLVNEGYYDESVLNRYSRDFVITLFAHAHTSGFRFQTF
                     LGAWKFYTSYTLKTFDGKRYLEDFADRVTMVALTLAQGDATLALQLTDEMLSGRFQPA
                     TPTFLNCGKQQRGELVSCFLLRIEDNMESIGRAVNSALQLSKRGGGVAFLLSNLREAG
                     APIKRIENQSSGVIPVMKMLEDAFSYPNQLGARQGAGAVYLHAHHPDILRFLDTKREN
                     ADEKIRIKTLSLGVVIPDITFHLAKENAQMALFSPYDVERVYGKPFADIAISEHYDEL
                     VADERIRKKYLNARDFFQRLAEIQFESGYPYIMYEDTVNRANPIAGRINMSNLCSEIL
                     QVNSASEYDENLDYARTGHDISCNLGSLNIAHTMDSPNFARTVETAVRGLTAVSDMSH
                     IRSVPSIEAGNAASHAIGLGQMNLHGYLAREGIAYGSPEALDFTNLYFYTITWHALRT
                     SMLLARERGETFAGFKQSRYASGEYFSQYLQGNWQPKTAKVGELFARSGITLPTREMW
                     AQLRDDVMRYGIYNQNLQAVPPTGSISYINHATSSIHPIVAKVEIRKEGKTGRVYYPA
                     PFMTNENLALYQDAYEIGAEKIIDTYAEATRHVDQGLSLTLFFPDTATTRDINKAQIY
                     AWRKGIKTLYYIRLRQMALEGTEIEGCVSCAL"
     gene            complement(3392..3802)
                     /gene="nrdI"
                     /locus_tag="ACHOSC_RS20635"
                     /old_locus_tag="pgaptmp_004127"
     CDS             complement(3392..3802)
                     /gene="nrdI"
                     /locus_tag="ACHOSC_RS20635"
                     /old_locus_tag="pgaptmp_004127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_process: GO:0009263 - deoxyribonucleotide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class Ib ribonucleoside-diphosphate reductase
                     assembly flavoprotein NrdI"
                     /protein_id="HGU4549951.1"
                     /translation="MSQLVYFSSSSENTQRFIERLGLPAVRIPLNERERIQVDEPYIL
                     IVPSYGGGGTAGAVPRQVIRFLNDEHNRALLRGVIASGNRNFGEAYGRAGDVIARKCG
                     VPWLYRFELMGTQSDIENVRKGVTEFWQRQPQNA"
     gene            complement(3799..4044)
                     /gene="nrdH"
                     /locus_tag="ACHOSC_RS20640"
                     /old_locus_tag="pgaptmp_004128"
     CDS             complement(3799..4044)
                     /gene="nrdH"
                     /locus_tag="ACHOSC_RS20640"
                     /old_locus_tag="pgaptmp_004128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin-like protein NrdH"
                     /protein_id="HGU4549952.1"
                     /translation="MRITIYTRNDCVQCHATKRAMENRGFDFEMINVDRVPEAAEALR
                     AQGFRQLPVVIAGDLSWSGFRPDMINRLHPAPHAASA"
     gene            complement(4292..4621)
                     /locus_tag="ACHOSC_RS20645"
                     /old_locus_tag="pgaptmp_004129"
     CDS             complement(4292..4621)
                     /locus_tag="ACHOSC_RS20645"
                     /old_locus_tag="pgaptmp_004129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311560.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF883 domain-containing protein"
                     /protein_id="HGU4549953.1"
                     /translation="MFNRPNRNDVDDGVQDIQNDVNQLADSLESVLKSWGSDAKGEAE
                     AARSKAQALLKETRARMHGRTRVQQAARDAVGCADSFVRERPWCSVGTAAAVGIFIGA
                     LLSMRKS"
     gene            4773..5117
                     /locus_tag="ACHOSC_RS20650"
                     /old_locus_tag="pgaptmp_004130"
     CDS             4773..5117
                     /locus_tag="ACHOSC_RS20650"
                     /old_locus_tag="pgaptmp_004130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2002 family protein"
                     /protein_id="HGU4549954.1"
                     /translation="MYLRPDEVARVLEKVGFTVDVVTQKAYGYRRGENYVYVNREARM
                     GRTALVIHPTLKERSSTLAEPASDIKTCDHYQQFPLYLAGERHEHYGIPHGFSSRVAL
                     ERYLNGLFGEAS"
     gene            complement(5154..5603)
                     /gene="alaE"
                     /locus_tag="ACHOSC_RS20655"
                     /old_locus_tag="pgaptmp_004131"
     CDS             complement(5154..5603)
                     /gene="alaE"
                     /locus_tag="ACHOSC_RS20655"
                     /old_locus_tag="pgaptmp_004131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-alanine exporter AlaE"
                     /protein_id="HGU4549955.1"
                     /translation="MFSPQSRLRHAVADTFAMVVYCSVVNMCIEVFLSGMSFEQSFYS
                     RLVAIPANILIAWPYGMYRDLFMRAARKVSPSGWIKNLADILAYVTFQSPVYVAILLV
                     VGADWHQIMAAVSSNIVVSMLMGAVYGYFLDYCRRLFKVSRYQQVKA"
BASE COUNT         1469 a         1415 c         1433 g         1436 t
ORIGIN      
        1 aaatagtcac ttttttctac cctaacatac tgagaatctg aggcaacccc tgagggcaaa
       61 tgtggcatga atattgatgc gagcaaatcg ccggaatttc ccgtgatata agggctgaga
      121 gcaaatcgag aaaagttgat attttttgtc atagatagcg aaaaaacgcc tgaatattgt
      181 agtttcaggc gaataatgca acagagtatc agtgtagatc actacagaat atttgcgtga
      241 taaaaagcta tttggcggga attatttccc tgctgcgggt agtgatattt ttgaaaataa
      301 caccctcaga aattccagtc ttcatcttct gtttcaaccg ctttccccat cacataagag
      361 gagcctgaac cggaaaagaa atcgtgattt tcatcggcat tcggcgaaag cgcggcgagg
      421 attgccggat tcacttccgc catttctgcg ggaaataacg gttcgtagcc cagattcatc
      481 aaagccttat tggcgttgta acagagaaac gctttcacat cgtcagccca cggggtttcg
      541 gcgtacagct catcggtgta ttgcaactcg ttgtcgtaga gttccagcaa caaatcgaag
      601 gcgaaactct tcaactcttc acgttgtccc agagatatct tttccatgtt tttctgatac
      661 ttatagccta tgtagtaacc gtggactgct tcatcgcgga taatcagacg gatcagatct
      721 gcggtattgg tcagctttcc gcggctggaa aaatacatcg gcagccagaa accggaatag
      781 aacaaaaaag attcaagaaa cacactggcg attttctttt tcagcggatc atcaccgcga
      841 taatgttgct gaataatctg agcttttcgc tgcaacggtg cgttttcttc actccaggcg
      901 taggcggcat cgacatcttt ggtctgacac agcgtcgaga aaatcgaact gtaagagcgg
      961 gcatgaaccg cttccataaa gctgatattc gataatactg cctcttcatg aggcgtgagc
     1021 gcatcgggca tcagcgaagg cgcgccgata acattttgca gcgtgtcgag cagcgtcagg
     1081 ccagtaaaaa cgcgcattgt cagttgttgt tctacgacac ttaatgtctg ccatgcaggt
     1141 atatcgttcg acagtggcac cttttctggt agccagaaat tgctggtcag gcgattccac
     1201 acctccagat ctttatcgtc agatatcttg ttccagttga tggcgctgat acgtgagagt
     1261 ttcatagata ttccttaaag tgcacaggag acgcagcctt caatttcagt gccttccagc
     1321 gccatctgac gcaggcgaat gtaatagagc gttttgatac ctttgcgcca ggcgtaaatc
     1381 tgcgctttgt tgatatcgcg agtggtggcg gtatcgggga aaaacagcgt cagcgacaaa
     1441 ccctgatcga catggcgagt cgcttccgcg taggtgtcga tgatcttttc tgcgccgatt
     1501 tcgtaagcat cctgatacag cgccagattc tcgttagtca taaacggggc agggtagtaa
     1561 acgcgtcctg ttttgccctc tttgcgtatc tctactttcg ccacaatcgg atgaatactc
     1621 gacgtagcat ggttgatata agagatagaa ccggttggcg gcaccgcctg gagattctgg
     1681 ttgtatatgc cgtagcgcat cacgtcgtcg cgcagttgcg cccacatctc acgggtaggt
     1741 aacgtaatac cgctgcgggc aaacagttcg ccaactttcg ccgttttcgg ctgccagtta
     1801 ccttgcagat attggctgaa atattcacca ctggcatagc gtgactgttt gaacccggcg
     1861 aaggtttcac cgcgttcgcg cgccagcaac atcgaggtac gcagtgcgtg ccaggtgatg
     1921 gtatagaaat agagattggt gaaatccagg gcttccggcg aaccataagc gatgccttcc
     1981 ctcgccagat aaccatgtaa attcatctgc cccagtccga tggcgtgcga ggcggcattt
     2041 ccggcttcga tggacggcac gctgcggata tgactcatat ctgacaccgc cgttaaaccg
     2101 cgtacggcag tctctaccgt gcgggcaaag ttgggggaat ccatggtgtg ggcaatattc
     2161 aacgaaccta aattgcagga aatatcatgg cctgtacggg catagtcgag attctcgtca
     2221 tactctgagg cgctgttaac ctgcaaaatt tctgagcaga gattactcat atttatgcgc
     2281 ccggcgatag ggttagcacg gtttaccgtg tcttcataca tgatgtaggg atagccggac
     2341 tcaaactgga tttctgccag tcgctggaag aaatcacggg cgttgaggta ttttttgcgg
     2401 atgcgttcat cggcaaccag ttcgtcatag tgttcgctaa tggcgatatc ggcaaacggc
     2461 ttgccataaa ctcgctcaac gtcatagggc gaaaacagcg ccatctgcgc attctctttt
     2521 gccagatgga aagtgatatc cgggatcacc acgccaagcg acagtgtttt aatgcggatt
     2581 ttttcgtcgg cattttcccg tttcgtgtcg agaaaacgca gaatatcggg atgatgagca
     2641 tgtaaataga ctgcaccagc cccctgacgc gcgccgagtt ggttgggata ggaaaatgcg
     2701 tcttccagca ttttcatcac cggaattacg ccagaagatt gattttcaat acgtttaatt
     2761 ggcgcgcccg cttcccgcag attcgacagc aaaaatgcca cgccgccgcc gcgtttcgac
     2821 agttgcagtg cggaatttac cgcccggcca atcgactcca tattgtcttc aatacgcagc
     2881 agaaaacagg aaaccagttc gccgcgctgc tgcttaccgc agttgaggaa tgttggcgtg
     2941 gctggctgaa agcgtcctga cagcatttca tcggtcagtt gcaacgccag cgtcgcatcg
     3001 ccttgtgcca gcgtcagcgc caccatcgtg actcgatcgg caaaatcttc cagataacgt
     3061 ttaccgtcga atgtcttcaa cgtatagctg gtgtaaaact tccatgcccc gaggaatgtc
     3121 tggaaacgaa aaccgctggt gtgtgcgtgg gcaaacagcg taatgacaaa gtcgcgagag
     3181 tagcgattaa gaacgctttc atcatagtaa ccttcgttga ccagccagtt caggcgctgc
     3241 tgctggctac tgaaggtcac actgttcgga cgcacatgcg tcgcaataaa ggcgtcaacg
     3301 gcctggcggt ctttatcgaa ctgaatgcga cctgcgctat cgtagaggtt aagcatcgca
     3361 ttcagcgcgt ggtaatccat cgtttcctgc gtcaggcatt ctgcggttgt cgttgccaaa
     3421 attcggttac tcctttacga acgttttcga tatcgctttg cgtacccatg agttcaaaac
     3481 ggtacagcca cggcacgccg catttccggg caatcacatc tccggcgcgg ccatacgcct
     3541 caccaaagtt gcgattacca gaagcaataa cgccgcgaag caacgcccgg ttgtgctcgt
     3601 cgtttaaaaa gcgaattacc tgtcgtggca ccgcgccagc cgtaccgccg ccgccgtaag
     3661 agggcacgat caggatgtaa ggctcgtcta cctgaatccg ttcccgctca ttgagcggga
     3721 tgcgcaccgc gggcagacct aaacgttcga taaaacgctg cgtgttttcg gagctgctgg
     3781 agaagtagac gagctggctc atgcactggc cgcgtgtggc gctggatgca gacggttaat
     3841 catgtccgga cggaaaccag accagctaag atcgccagca atcactaccg gcaactgacg
     3901 aaagccctga gcacgcaacg cttccgctgc ttcaggaacg cgatcgacat taatcatttc
     3961 aaaatcaaag ccccggtttt ccatcgcccg tttggtggcg tggcactgaa cgcaatcgtt
     4021 acgagtgtaa atagtaatgc gcatgattcg tatttccgtt taaaatgaag atacggcgcg
     4081 atgatacgcg tcgggttgtc tctctgttga tacagagata ctagatgtag ttgaaaaaag
     4141 attcaaccac acaatatata gcaaatagtg gtcgaaatta ccctggatat gagcgtgacg
     4201 gggtaggggg atttttgtga ttcaccaggc aaaaagaaac cccgaagcca ggcttcgggg
     4261 tcaaagacgc gtatttatta tcatttttgc actacgattt gcgcatgctt aacagtgcgc
     4321 cgataaaaat acctaccgca gctgctgtac ccacgctaca ccagggtctt tcacgaacaa
     4381 aagaatccgc gcagccaacg gcatcgcgtg cggcttgctg gacgcgagta cgaccatgca
     4441 ttcgcgccct ggtttctttc agcaatgcct gagctttact gcgtgcggct tcagcttccc
     4501 ctttggcgtc gctgccccag gacttcaata cagattcgag gctgtcagct aattgattga
     4561 catcattctg gatatcctgc acgccatcat caacgtcgtt gcggttcggt cggttaaaca
     4621 tatgatcccc cattagaatt cgatgtaatt ttcagtgtag accataattt tcttaactga
     4681 ggttcatcgc ggctttatgg acttttctga aagcgttgtg aatgcgcaat caggtaaggt
     4741 aggcccgcgg ttgataataa cgaggatcaa atatgtattt acgaccagac gaggtggcgc
     4801 gcgtacttga aaaagtcggt tttactgtcg atgtggtaac ccagaaagcg tatggttacc
     4861 gccgtgggga aaattatgtc tatgttaatc gcgaagcgcg aatgggacgt accgctctgg
     4921 taattcatcc aacattaaaa gaacgcagtt caacactggc tgaacccgct tccgatatta
     4981 aaacttgcga tcattaccag caatttccgc tctatttagc aggcgagcga cacgagcatt
     5041 acggtatccc gcatggcttt agttcgcgtg ttgcgcttga acgttatttg aatggtttat
     5101 ttggcgaagc cagttaaaga cgcgactggc gatgccagtc gcgaaaagaa gagtcaggct
     5161 tttacctgct ggtaacggct gactttaaac agtcggcggc aataatcgag gaagtagcca
     5221 taaaccgccc ccatcaacat cgaaacaacg atgtttgaac tgaccgccgc cataatctga
     5281 tgccagtctg cgcccaccac taacaagatc gccacataca ccggtgactg gaacgtcaca
     5341 taagccagga tatcagccag attttttatc cagcccgacg ggctaacttt gcgtgccgcg
     5401 cgcataaaca gatcgcggta cataccgtat ggccatgcaa ttaagatgtt cgccggaatc
     5461 gctaccaatc tggaataaaa agactgttcg aagctcattc cggagaggaa aacttcaata
     5521 cacatgttca cgacagaaca gtaaacaacc atcgcgaacg tatctgcaac tgcatgacgc
     5581 aagcgtgact gcggtgagaa catgctaatg ctccttaata aaagcgtaat gggatgttaa
     5641 tggagatgaa agcagtggat ctcattgcca tctgcatttt cgcatagcgt atatcgctgg
     5701 ctatgactat tcaattagtg aaaaattttt atttagtcag tttttgtaga taa
//
LOCUS       DAZKHF020000268         5741 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2201S31241
            SAMN43267800-rid25106413.denovo.270, whole genome shotgun sequence.
ACCESSION   DAZKHF020000268 DAZKHF020000000
VERSION     DAZKHF020000268.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267800
            Sequence Read Archive: SRR30307263
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5741)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5741)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 5741)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350581.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350581.2
            Genome Coverage       :: 151x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:21:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,652
            CDSs (total)                      :: 5,557
            Genes (coding)                    :: 5,320
            CDSs (with protein)               :: 5,320
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 237
            CDSs (without protein)            :: 237
            Pseudo Genes (ambiguous residues) :: 0 of 237
            Pseudo Genes (frameshifted)       :: 102 of 237
            Pseudo Genes (incomplete)         :: 130 of 237
            Pseudo Genes (internal stop)      :: 52 of 237
            Pseudo Genes (multiple problems)  :: 41 of 237
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5741
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267800-rid25106413.denovo.270"
                     /serotype="O 26:H11"
                     /isolate="2201S31241"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-01"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            1..691
                     /locus_tag="ACHOSC_RS20660"
                     /old_locus_tag="pgaptmp_004132"
                     /pseudo
     CDS             1..691
                     /locus_tag="ACHOSC_RS20660"
                     /old_locus_tag="pgaptmp_004132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418441.1"
                     /note="internal stop; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="ShET2/EspL2 family type III secretion system
                     effector toxin"
     gene            complement(660..2396)
                     /gene="aceK"
                     /locus_tag="ACHOSC_RS20665"
                     /old_locus_tag="pgaptmp_004133"
     CDS             complement(660..2396)
                     /gene="aceK"
                     /locus_tag="ACHOSC_RS20665"
                     /old_locus_tag="pgaptmp_004133"
                     /EC_number="2.7.11.5"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008772 - [isocitrate dehydrogenase
                     (NADP+)] kinase activity [Evidence IEA];
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA];
                     GO_process: GO:0006006 - glucose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional isocitrate dehydrogenase
                     kinase/phosphatase"
                     /protein_id="HGU4549956.1"
                     /translation="MPRGLELLIAQTILQGFDAQYGRFLEVTSGAQQRFEQADWHAVQ
                     QAMKNRIHLYDHHVGLVVEQLRCITNGQSTDAAFLLRVKEHYTRLLPDYPHFEIAESF
                     FNSVYCRLFDHRSLTPERLFIFSSQPERRFRTIPRPLAKDFHPDHGWESLLMRVISDL
                     PLRLRWQNKSRDIHYIIRHLTETLGTDNLAESHLQVANELFYRNKAAWLVGKLITPSG
                     TLPFLLPIHQTDDGELFIDTCLTTTAEASIVFGFARSYFMVYAPLPAALVEWLREILP
                     GKTTAELYMAIGCQKHAKTESYREYLVYLQGCNEQFIEAPGIRGMVMLVFTLPGFDRV
                     FKVIKDKFAPQKEMSAAHVRACYQLVKEHDRVGRMADTQEFENFVLEKRHISPALMEL
                     LLQEAAEKITDLGEQIVIRHLYIERRMVPLNIWLEQVEGQQLRDAIEEYGNAIRQLAA
                     ANIFPGDMLFKNFGVTRHGRVVFYDYDEICYMTEVNFRDIPPPRYPEDELASEPWYSV
                     SPGDVFPEEFRHWLCADPRIGPLFEEMHADLFRADYWRALQNRIREGHVEDVYAYRRR
                     QRFSVRYGEMLF"
     gene            complement(2677..3981)
                     /gene="aceA"
                     /locus_tag="ACHOSC_RS20670"
                     /old_locus_tag="pgaptmp_004134"
     CDS             complement(2677..3981)
                     /gene="aceA"
                     /locus_tag="ACHOSC_RS20670"
                     /old_locus_tag="pgaptmp_004134"
                     /EC_number="4.1.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004451 - isocitrate lyase activity
                     [Evidence IEA];
                     GO_process: GO:0019752 - carboxylic acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isocitrate lyase"
                     /protein_id="HGU4549957.1"
                     /translation="MKTRTQQIEELQKEWTQPRWEGITRPYSAEDVVKLRGSVNPECT
                     LAQLGAAKMWRLLHGESKKGYINSLGALTGGQALQQAKAGIEAVYLSGWQVAADANLA
                     ASMYPDQSLYPANSVPAVVERINNTFRRADQIQWSAGIEPGDPRYVDYFLPIVADAEA
                     GFGGVLNAFELMKAMIEAGAAAVHFEDQLASVKKCGHMGGKVLVPTQEAIQKLVAARL
                     AADVTGVPTLLVARTDADAADLITSDCDPYDSEFITGERTSEGFFRTHAGIEQAISRG
                     LAYAPYADLVWCETSTPDLELARRFAQAIHAKYPGKLLAYNCSPSFNWQKNLDDKTIA
                     SFQQQLSDMGYKFQFITLAGIHSMWFNMFDLANAYAQGEGMKHYVEKVQQPEFAAAKD
                     GYTFVSHQQEVGTGYFDKVTTIIQGGTSSVTALTGSTEESQF"
     gene            complement(4011..5612)
                     /gene="aceB"
                     /locus_tag="ACHOSC_RS20675"
                     /old_locus_tag="pgaptmp_004135"
     CDS             complement(4011..5612)
                     /gene="aceB"
                     /locus_tag="ACHOSC_RS20675"
                     /old_locus_tag="pgaptmp_004135"
                     /EC_number="2.3.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004474 - malate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006097 - glyoxylate cycle [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malate synthase A"
                     /protein_id="HGU4549958.1"
                     /translation="MTEQATTTDELAFIRPYGEQEKQILTAEAVEFLTELVTHFTPQR
                     NKLLAARIQQQQDIDNGTLPDFISETASIRDTDWKIRGIPADLQDRRVEITGPVERKM
                     VINALNANVKVFMADFEDSLAPDWNKVIDGQINLRDAVNGTISYTNEAGKIYQLKPNP
                     AVLICRVRGLHLPEKHVTWRGEAIPGSLFDFALYFFHNYQALLAKGSGPYFYLPKTQS
                     WQEAAWWSEVFSYAEDRFNLPRGTIKATLLIETLPAVFQMDEILHALRDHIVGLNCGR
                     WDYIFSYIKTLKNYPDRVLPDRQAVTMDKPFLNAYSRLLIKTCHKRGAFAMGGMAAFI
                     PSKDEERNNQVLNKVKADKALEANNGHDGTWIAHPGLADTAMAVFNDILGSRKNQLEV
                     MREQDAPITADQLLAPCDGERTEEGMRANIRVAVQYIEAWISGNGCVPIYGLMEDAAT
                     AEISRTSIWQWIHHQKTLSNGKPVTKALFRQMLGEEMKVIASELGEERFSQGRFDDAA
                     RLMEQITTSDELIDFLTLPGYRLLA"
BASE COUNT         1393 a         1513 c         1422 g         1413 t
ORIGIN      
        1 gaatgtagat tatagcctcc tgaaatggtc aaatgatcat attattaacc aatctgttgc
       61 aataattcca gcacttccga aagaacagct attgatgtta aaaggatctg tggatgaaat
      121 aacccctcca ttatcaccag caacgatgaa tttgctaatg gcaattggtc agaatcacca
      181 acttacgcaa ctgatgattc agctccagaa aatgccagaa ctacatagaa cagaaatgtt
      241 gactgcctat aatagtggac atatgaacgt tattaatact atttttaacg cataacccac
      301 tctgtttaat acgtttaaat tcgataaaaa aaatatgaag cccctcctcc tggcaaataa
      361 ttctaatgaa tatcccggtt tgttttcagc gatacagcat aaacaacaaa atgttgtaga
      421 gacggtttat cttgctttat ctaaccatgc acgcctgttt ggatttaccg ctgaagatat
      481 tatggatttt tggcaacaca aagccccaca aaaatactct gcctttgagt tggcttttga
      541 attgggtcac cgggttattg ctgaattaat ccttaataca ttaaataaga tggctgaaag
      601 ctttggcttt acggataacc ctcgatacat tgcggagaaa aattatatgg aagctttact
      661 caaaaaagca tctccccata ccgtacgcta aatctttgcc tgcgccgata cgcataaaca
      721 tcttccacat gcccttcacg tatgcggttt tgtagtgcgc gccagtaatc agcgcggaac
      781 aggtcggcgt gcatctcttc aaacagcgga ccaatacgcg ggtcggcgca tagccagtgg
      841 cgaaactctt ccgggaaaac atcgcccggc gagacgctgt accacggttc gctggcaagt
      901 tcgtcttccg gatagcgcgg cggcgggatg tcgcggaaat tcacttccgt catgtagcaa
      961 atttcatcgt aatcataaaa aaccacacgc ccgtgacggg tgacaccgaa gtttttaaac
     1021 agcatgtcgc caggaaaaat gttagcagcg gcaagctggc gaatagcgtt accgtattct
     1081 tcaatggcgt cgcgcaactg ctgaccttcc acttgttcca gccagatatt gagcggcacc
     1141 atccgccgct cgatatagag atggcgaatt acaatctgct caccaagatc ggtgattttt
     1201 tccgctgctt cctggagcag taattccatt aatgccgggg aaatatgccg cttctccagc
     1261 acaaagtttt caaactcctg ggtgtccgcc attcggccca cacggtcgtg ctctttcacc
     1321 agttgatagc aggcacgaac gtgagcggca gacatctctt tctgcggcgc gaacttgtct
     1381 ttgatgactt tgaatacccg atcaaagccc ggcagcgtaa ataccaacat caccattcca
     1441 cgaatacccg gcgcttcaat gaactgctca ttacagccct gtagataaac gagatattcg
     1501 cggtagcttt cggttttggc gtgcttctgg cagccgatag ccatatacaa ttcagcagtg
     1561 gttttacccg gcagaatttc ccgtagccac tcgaccagcg ctgcgggcag cggtgcataa
     1621 accataaaat aagaacgcgc aaagccaaaa acaatgctcg cttcggcggt cgtcgtcagg
     1681 caggtatcaa taaataactc gccgtcgtcc gtctggtgga tcggcagtaa gaagggcaac
     1741 gtgccggaag gcgtgatcag tttgcccacc agccaagcgg ctttattgcg gtaaaacagt
     1801 tcgttcgcca cctgtaaatg actttccgcg aggttgtctg tccccagcgt ttccgtcaga
     1861 tggcgaatga tgtaatgaat gtcacggctt ttattctgcc agcgcaggcg cagcggcagg
     1921 tcgctgataa cgcgcatcag tagagattcc cagccgtgat cggggtgaaa gtctttcgcc
     1981 agcgggcggg gaatggtacg aaagcggcgc tctggctgag agctaaagat aaaaagccgc
     2041 tcgggagtaa gcgagcggtg gtcaaataac cgacagtaca cggagttaaa aaagctctcc
     2101 gcaatctcga agtgcgggta atccggcaac agccgcgtgt aatgctcttt aacgcgcagt
     2161 aaaaatgccg cgtccgtact ttggccgtta gtaatgcagc gcagttgctc cacgaccaga
     2221 ccaacgtgat gatcgtaaag atggatacgg tttttcatcg cctgctggac agcatgccag
     2281 tcggcctgtt cgaaacgctg ctgcgccccg gaagtcactt cgaggaatcg accatactga
     2341 gcgtcgaagc cttgcaaaat ggtttgagca atcagtaatt ccaggccacg cggcatttag
     2401 cgccctcatc aggagcagag aattgccgga tgcggcgtaa acgccttatc cagcctacgt
     2461 tcggcaacgg ttgtaggcat gataagacgc gcaagcgtcg catcaggcat cgtgcaccga
     2521 ttgccggatg cggcgtaaat gccttatccg gcctacgttc ggcaacggct gtaggcatga
     2581 taagacgcgc aagcgtcgca tcaggcatcg tgcaccgatt gccggatgcg gcgtgaacgc
     2641 cttatccggc ctacagtcag caacggttgt tgttgcttag aactgcgatt cttcagtgga
     2701 gccggtcagc gcggtgactg aagacgtgcc gccctgaata atagtcgtca ctttatcgaa
     2761 gtaacctgta cccacttcct gctggtgaga tacgaaggta tagccatctt tcgcggcggc
     2821 aaattccggc tgctgcactt tctcaacgta gtgcttcata ccctcgccct gggcataggc
     2881 gtttgccagg tcaaacatgt tgaaccacat gctgtggata cctgccaggg tgatgaactg
     2941 gaacttgtag cccatatccg acagctgctg ctggaagctg gcaatagttt tgtcgtcgag
     3001 gtttttctgc cagttgaacg acggcgagca gttataagcc agcagtttgc ccggatattt
     3061 cgcgtggata gcttgtgcaa agcgacgcgc cagttccaga tccggcgtgg aggtttcaca
     3121 ccagaccagg tcagcatatg gcgcatacgc caggccacgg ctgatcgctt gctcaatgcc
     3181 cgcatgagta cggaagaagc cttcactggt acgctcgcca gtaataaatt cactgtcata
     3241 cgggtcgcaa tcggaggtaa tcagatccgc cgcatcggca tcggtacgag caaccagcag
     3301 ggttggaacg cccgtcacgt cagctgcaag acgcgccgcg accagtttct gaatagcttc
     3361 ctgagttggc accaaaactt tgccgcccat gtgaccgcat ttcttcactg acgccagctg
     3421 atcttcgaag tgaactgccg ctgcaccggc ttcaatcatc gctttcatca gttcaaaggc
     3481 attcaggaca ccgccaaaac cggcttccgc atcggcaacg atcggcagga agtaatcgac
     3541 atagcgcgga tcgcccggct caatgcctgc ggaccattgg atctgatcgg cacgacggaa
     3601 ggtgttgttg atccgctcca ccacagctgg caccgagttt gccggataga gcgactgatc
     3661 cggatacatg ctggccgcca ggttagcgtc cgccgctacc tgccatcccg acagatagac
     3721 tgcttcaata cccgctttcg cctgttgcag cgcctgaccg ccagtcagtg caccgaggct
     3781 gttgatgtag ccttttttcg actcgccatg cagcagacgc cacattttcg ccgcgcccag
     3841 ttgcgccagg gtgcattcag gattgactga accgcgtaat ttcaccacat cttccgcgct
     3901 gtatgggcga gtaatgcctt cccaacgcgg ttgagtccac tctttctgta attcttcaat
     3961 ttgttgtgta cgggttttca tgtgcagatg ctccatagtt atgtggtggt ttacgctaac
     4021 aggcggtagc ctggcagggt caggaaatca attaactcat cggaagtggt gatctgttcc
     4081 atcaagcgtg cggcatcgtc aaaacgcccc tgggagaaac gttcttcgcc cagttcgctg
     4141 gcaatgactt tcatctcttc acccagcatc tggcggaaca aggctttggt caccggtttg
     4201 ccattgctca acgttttttg atgatggatc cactgccaga ttgaggtacg ggaaatttca
     4261 gccgtcgccg catcttccat caggccataa atcggcacgc agccgttgcc ggagatccat
     4321 gcttcgatgt actgcactgc cacgcgaatg ttggcgcgca taccttcttc ggtgcgttca
     4381 ccatcacaag gtgccagcag ctgatcggca gtaatcggcg cgtcttgttc gcgcatcact
     4441 tcaagctgat ttttacggga gccgagaatg tcgttgaata ccgccattgc cgtatccgca
     4501 aggcctgggt gagcaatcca tgtgccatcg tgaccgttat tggcttccag cgctttatcc
     4561 gcttttactt tgttgagcac ctggttattg cgctcttcat ctttgctcgg aataaacgct
     4621 gccatgccgc ccatcgcaaa agcaccgcgt ttatggcagg ttttaatcaa caggcgtgag
     4681 taagcattca ggaatggttt atccatcgtc actgcctgtc tgtctggcag gacgcgatcg
     4741 ggatagtttt tcaacgtttt gatatagctg aagatgtaat cccaacgacc gcagttcaga
     4801 ccaacaatat ggtcacgcag cgcgtgaagg atttcatcca tctggaacac ggcgggcagc
     4861 gtttcaatca gcaacgtcgc cttgatggtg ccgcgcggca gattaaagcg atcttctgca
     4921 tagctgaaga cttcgctcca ccaggccgct tcctgccagg actgggtttt cggcagatag
     4981 aaatagggac cgctgccctt tgccaacagt gcctgatagt tgtggaagaa atagagcgca
     5041 aaatcaaaca ggctaccggg gattgcctca ccacgccagg tgacatgttt ttccggcaag
     5101 tgcagaccgc gtacccgaca aatcaaaacc gctggattgg gcttgagctg ataaattttg
     5161 cctgcttcat tggtatagct gatggtgccg ttaaccgcat cgcgcaggtt aatttgcccg
     5221 tcgatcactt tgttccagtc tggtgccagt gaatcttcga aatcggccat aaagactttc
     5281 acattggcgt tcagtgcgtt gatcaccatc ttgcgctcaa ccgggccagt tatctcgact
     5341 cgacgatctt gtaagtccgc gggaatacca cgaattttcc agtccgtatc gcgaatggaa
     5401 gctgtttccg aaataaaatc aggcaaagtt ccgttatcaa tatcttgctg ctgctgaatg
     5461 cgcgctgcca gaagtttatt gcgttgtggc gtaaaatgcg tcaccagctc agtcagaaat
     5521 tctaccgctt cggcagtaag aatttgcttc tcctgctcgc catacggcct tatgaaagcc
     5581 agttcatcgg ttgttgttgc ctgttcagtc atcgtgcagc tcctcgtcat ggacccgaaa
     5641 acttccccac tgtgaacgaa ggatcgttgt gcgcttttcg ttcagcacaa ctaagactac
     5701 tcatttaaaa tgcaaaatca aaaacaattt ccattttaat t
//
LOCUS       DAZKHF020000269         5735 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2201S31241
            SAMN43267800-rid25106413.denovo.271, whole genome shotgun sequence.
ACCESSION   DAZKHF020000269 DAZKHF020000000
VERSION     DAZKHF020000269.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267800
            Sequence Read Archive: SRR30307263
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5735)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5735)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 5735)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350581.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350581.2
            Genome Coverage       :: 151x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:21:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,652
            CDSs (total)                      :: 5,557
            Genes (coding)                    :: 5,320
            CDSs (with protein)               :: 5,320
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 237
            CDSs (without protein)            :: 237
            Pseudo Genes (ambiguous residues) :: 0 of 237
            Pseudo Genes (frameshifted)       :: 102 of 237
            Pseudo Genes (incomplete)         :: 130 of 237
            Pseudo Genes (internal stop)      :: 52 of 237
            Pseudo Genes (multiple problems)  :: 41 of 237
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5735
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267800-rid25106413.denovo.271"
                     /serotype="O 26:H11"
                     /isolate="2201S31241"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-01"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            complement(196..1371)
                     /locus_tag="ACHOSC_RS20680"
                     /old_locus_tag="pgaptmp_004136"
     CDS             complement(196..1371)
                     /locus_tag="ACHOSC_RS20680"
                     /old_locus_tag="pgaptmp_004136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000516612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3596 domain-containing protein"
                     /protein_id="HGU4549959.1"
                     /translation="MGKKEQGLLSLPRGVTIRKHKTASTLVITFTYKGVLCREPLSRL
                     EANTRGIKYAERLLGEIQNQIASGTFEYAKYFPNSKKLELFGVVKKTKNIKSYLDEYL
                     KICINRNLSPSTIGGYEKCLSALSELHKLHVTELTPAILKNWIASRKTKLKTIRNNLS
                     FLRSAIDEAVTDGLLTINPVTLVSASRYHVIDSSPSADDYEVDPFTPAETLAIYQSCR
                     YREWENLFRFAFNTGLRSSELCALRWADLDTIANTAHVQAASVVGVLKGTKTKAGTRK
                     VELNSEALAALQAQKQYTFMKSEFIFSDPKTGEPWTNADAIRKKAWVPTLKKAGVRYR
                     NPYQTRHTFATRHISQGVNLFWLAGQMGHKGPEMLFRNYGKYLAEYDGKTSINVVSS"
     gene            complement(1326..1535)
                     /locus_tag="ACHOSC_RS20685"
                     /old_locus_tag="pgaptmp_004137"
     CDS             complement(1326..1535)
                     /locus_tag="ACHOSC_RS20685"
                     /old_locus_tag="pgaptmp_004137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000050710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excisionase"
                     /protein_id="HGU4549960.1"
                     /translation="MGLDCVPISTYCRNAGETVDAVNKRIQRGIWKEGVHVLKVDGVK
                     ERWVDLIEVSKWARKNKDYYLCQEE"
     gene            complement(1544..1690)
                     /locus_tag="ACHOSC_RS20690"
                     /old_locus_tag="pgaptmp_004138"
     CDS             complement(1544..1690)
                     /locus_tag="ACHOSC_RS20690"
                     /old_locus_tag="pgaptmp_004138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001030144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4549961.1"
                     /translation="MHTVFELWVRKTFGNRYDLTRDVDGFYCREIVKRMFEVWCHCRG
                     LNVV"
     gene            complement(1694..1936)
                     /locus_tag="ACHOSC_RS20695"
                     /old_locus_tag="pgaptmp_004139"
     CDS             complement(1694..1936)
                     /locus_tag="ACHOSC_RS20695"
                     /old_locus_tag="pgaptmp_004139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4222 domain-containing protein"
                     /protein_id="HGU4549962.1"
                     /translation="MFALIQRGQIYTDRAGYPVVITRCTDRSVFFRRMDGHSGRVCIG
                     EFNCLFEHIDHQQYCKILADTEQEKHLKKLRAMQRR"
     gene            complement(2021..2185)
                     /locus_tag="ACHOSC_RS20700"
                     /old_locus_tag="pgaptmp_004140"
     CDS             complement(2021..2185)
                     /locus_tag="ACHOSC_RS20700"
                     /old_locus_tag="pgaptmp_004140"
                     /inference="COORDINATES: protein motif:HMM:NF016343.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF551 domain-containing protein"
                     /protein_id="HGU4549963.1"
                     /translation="MPNDAQWCVVNTEYGYYVQCWSEGQGWLGDDISIPECDVTHWMP
                     LPEPPQEVSQ"
     gene            complement(2273..2536)
                     /locus_tag="ACHOSC_RS20705"
                     /old_locus_tag="pgaptmp_004141"
                     /pseudo
     CDS             complement(2273..2536)
                     /locus_tag="ACHOSC_RS20705"
                     /old_locus_tag="pgaptmp_004141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310783.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="Eaa protein"
     gene            complement(2759..2884)
                     /locus_tag="ACHOSC_RS20710"
                     /old_locus_tag="pgaptmp_004142"
                     /pseudo
     CDS             complement(2759..2884)
                     /locus_tag="ACHOSC_RS20710"
                     /old_locus_tag="pgaptmp_004142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416864.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="Eaa protein"
     gene            complement(2886..3215)
                     /locus_tag="ACHOSC_RS20715"
                     /old_locus_tag="pgaptmp_004143"
     CDS             complement(2886..3215)
                     /locus_tag="ACHOSC_RS20715"
                     /old_locus_tag="pgaptmp_004143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000014163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ead/Ea22-like family protein"
                     /protein_id="HGU4549964.1"
                     /translation="MSKIDYQALREAAEAIKVVATPQKLLAFRMKVTPQVVLVLLDEL
                     EAKNKRITELEAREVQLPTRYDLRYGHPINADKRHVMIPKENGSWLCLIDLEHALRVA
                     GIRIKGE"
     gene            complement(3212..3451)
                     /locus_tag="ACHOSC_RS20720"
                     /old_locus_tag="pgaptmp_004144"
     CDS             complement(3212..3451)
                     /locus_tag="ACHOSC_RS20720"
                     /old_locus_tag="pgaptmp_004144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000476205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4549965.1"
                     /translation="MFKQNEKAIAQIADYIPRACRGMQLQEAKARLEKKIALYIDDGC
                     DAAVLNAAFAPALNSHTREAFFSRIAAQIRKGGNQ"
     gene            complement(3444..3647)
                     /locus_tag="ACHOSC_RS20725"
                     /old_locus_tag="pgaptmp_004145"
     CDS             complement(3444..3647)
                     /locus_tag="ACHOSC_RS20725"
                     /old_locus_tag="pgaptmp_004145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019705292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4549966.1"
                     /translation="MTIVKTHTGTVITKDGPQVKKLHQTERMWVVGKNEFYHKETGRR
                     HFAENTRRRLLLDTIKPIEVKHV"
     gene            complement(3644..4522)
                     /locus_tag="ACHOSC_RS20730"
                     /old_locus_tag="pgaptmp_004146"
     CDS             complement(3644..4522)
                     /locus_tag="ACHOSC_RS20730"
                     /old_locus_tag="pgaptmp_004146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000335005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoadenosine phosphosulfate reductase family
                     protein"
                     /protein_id="HGU4549967.1"
                     /translation="MCITHVVSFSGGRTSAYLVHLMEEQRKAGNNVCYIFMDTGCEHP
                     LTYRFIREVLKFWGIPLTVLQVDINPELGQPNGYTEWEPKDIQTRMPVLKPFMDMVKK
                     YGTPYIGGAFCTDRLKLTPFTKYCDDHFGRGNYITWLGIRADEPRRLKPKPGVRYLAE
                     LSDFDKSDVIRWWQKQPFDLQIPEHLGNCVFCIKKSTQKLGLACKDEPGLMRVFNELV
                     TGKHVRDGHRRTGKDVMYRGHLTLDGIARMYADSDYRSLYQAMVLAKRFDTGSCSESC
                     EIWGDQLELKFEEVVA"
     gene            complement(4513..5049)
                     /gene="yfdR"
                     /locus_tag="ACHOSC_RS20735"
                     /old_locus_tag="pgaptmp_004147"
     CDS             complement(4513..5049)
                     /gene="yfdR"
                     /locus_tag="ACHOSC_RS20735"
                     /old_locus_tag="pgaptmp_004147"
                     /EC_number="3.1.3.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416862.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5'-deoxynucleotidase"
                     /protein_id="HGU4549968.1"
                     /translation="MSFIKTFSGKHFYYDKINKDDIVINDIAVSLSNICRFAGHLSHF
                     YSVAQHAVLCSQLVPQEFAFEALMHDATEAYCQDIPAPLKRLLPDYKRMEEKIDAVIR
                     EKYGLPPVMSTPVKYADLIMLATERRDLGLDDGSFWPVLEGIPATEMFKVIPLAPGHA
                     YGMFMERFNELSELRKCA"
     gene            complement(5178..>5735)
                     /locus_tag="ACHOSC_RS20740"
                     /old_locus_tag="pgaptmp_004148"
     CDS             complement(5178..>5735)
                     /locus_tag="ACHOSC_RS20740"
                     /old_locus_tag="pgaptmp_004148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2303 family protein"
                     /protein_id="HGU4549969.1"
                     /translation="AVSVLNLGTIDEPGHADNTATLKLKKTAPFSALLSVNGERNSQK
                     SLAEWIEDWADYLVGFDANGDTIQATKAAAAVRKITIEANQTADFEDNDFSGKRSLME
                     SVEAKTKDIMPVAFEFKCVPFEGLKERPFKLRLSIITGDRPVLVLRIIQLEAMQEEMA
                     NEFRDLLVEKFKDSKVETFIGTFTA"
BASE COUNT         1381 a         1458 c         1267 g         1629 t
ORIGIN      
        1 aataaagtaa cttacgtgaa gtcaagaaat atatttgcac tccatttctt gctatgatgt
       61 aagttattga ttttaattaa atttacaatt acctaaatga actatggaaa ctatcaataa
      121 catagatgat agctcatttc acaaaaagca atggtagact cattcttaat tttcatcagc
      181 cttaagccta tgtactcagc ttgacacaac atttatagac gttttaccat cgtattcagc
      241 caggtattta ccgtaattgc ggaacagcat ttccggcccc ttatgcccca tctgcccggc
      301 aagccagaaa aggtttacac cctggctaat gtgtctggtg gcgaatgtgt ggcgcgtctg
      361 gtacgggtta cggtagcgca caccagcttt tttcagggtc ggcacccatg cttttttacg
      421 gatagcgtcg gcgttcgtcc agggttctcc cgttttcgga tcgctgaata tgaactcact
      481 tttcataaag gtgtattgct tctgcgcctg cagggctgcc agcgcctcac tgttcagctc
      541 caccttacgg gtaccggctt ttgtcttggt gcctttaagt acccctacga cactggccgc
      601 ctgaacgtgg gctgtgttcg cgatggtgtc gagatcagcc cagcgcagcg cgcacagttc
      661 tgagctccgc agacctgtat tgaaagcaaa gcggaacagg ttttcccatt ccctgtacct
      721 gcagctctgg taaatggcaa gggtctccgc tggcgtgaac gggtcaacct cgtaatcgtc
      781 ggcgctcggg ctactgtcga tcacgtggta ccggctggcg ctgacgaggg ttaccgggtt
      841 aatggtcagc aggccatccg taacagcttc atcaatggcg ctgcgcagaa atgacaggtt
      901 attcctgatt gttttcagct tagtttttcg gctggctatc caatttttta gtatcgcagg
      961 cgtcaattct gttacatgta gtttatgtag ttctgatagt gctgatagac atttttcgta
     1021 accgccaatg gttgacggcg acagattacg gtttatgcaa attttcaggt attcatcaag
     1081 ataggactta atatttttgg ttttctttac tacaccgaat aactccagtt ttttggaatt
     1141 ggggaaatat ttcgcatatt caaacgttcc gctggcgatc tgattttgta tctcccctag
     1201 caggcgctca gcatatttta taccacgtgt atttgcctca agccgagata ggggctccct
     1261 gcaaagaacc cctttatatg tgaaagtgat aactagtgtt gaagcagttt tatgcttacg
     1321 aatagttact cctcttggca gagataataa tccttgttct ttcttgccca ttttgaaacc
     1381 tctattaagt cgacccaacg ctctttaacc ccgtcgactt ttaatacatg taccccttct
     1441 ttccatattc ctctttgtat ccgtttgtta acggcatcaa ccgtttctcc cgcgttgcgg
     1501 cagtaggttg atataggtac gcaatccagc cccacagttc acctcacaca acattcagcc
     1561 cacgacagtg acaccacact tcaaacattc gtttcacaat ttcacgacag tagaaaccgt
     1621 caacatctcg cgtcaggtca tagcgattgc cgaatgtctt gcgaacccat aactcaaaaa
     1681 ccgtatgcat gtatcacctc cgttgcattg cgcgcaattt tttcaggtgc ttttcctgct
     1741 cagtgtccgc caggattttg caatactgct ggtggtcaat atgttcgaac aggcagttga
     1801 actcaccaat gcatacccgc ccggagtgtc cgtccatacg tcgaaagaac acagagcggt
     1861 ccgtacagcg agtaatcacc acagggtatc cggccctgtc cgtgtatatt tgcccgcgtt
     1921 gaataagtgc gaacatgaga ttatccccag cggcaaatcg aaaaaataat cagcgcaatc
     1981 gatattgcaa cacctacaat tgcaaatgct tcaggccaac tcattgactc acctcctgcg
     2041 gcggttctgg tagaggcatc cagtgggtta catcgcattc agggatgctg atatcatcac
     2101 caagccaccc ttgaccttca gaccagcatt gcacgtaata cccgtattct gtgttcacta
     2161 cgcaccactg tgcgtcgttc ggcattagct cactacagct tatccaacca tccggagtta
     2221 ccggaacttg cggaatggct gtctgctctc gaacgtcatt aggcgctata ggttctgctg
     2281 ccaactgact ggcatatttg ttaatggtaa cgataagctc ttgctcagcc tcatccagat
     2341 aatcaccgat acctcgcctg tcaccgtcaa aatcatcgaa atcggcacga atcttggcaa
     2401 ccttctggat tgcggacaac acctcactag gaattaccgg atagttggtt gacgtttccg
     2461 cgatttcccg aaaattattg gttgacgaat tcttattttc ccgaaagttt ctggcctgaa
     2521 gcatggcggc gcggcaggca ttccagccag tagcgtaata tttcgcatcc tgccagctcg
     2581 tttgtacatt tgagtacgct ttttcctctg tcatggcctc tggtattgat gccggtgcaa
     2641 ttcgtggcag gccgatatat agtggtacat ctcccggctc catcgaattg acgggccaaa
     2701 tatacgcatc actaccatgg ttaatgagcg caatccccga agtatcgatg atgccaaatg
     2761 gctccgctgt caatgcagtc aatgcaattt cataagcccg acgctcaata ttgtctcgca
     2821 cgtccaggct gcttatgcgc tctttgattt ctttaatcag tttcttatcg gtgaaagtgg
     2881 tcatctcact ctcctttgat gcgaatgcca gcgacgcgta gtgcgtgttc taagtcaatc
     2941 aggcaaagcc agctgccatt ttctttaggt atcatgacat gtcgcttatc agcatttatt
     3001 gggtgcccat atcgaaggtc gtagcgagtc ggtaattgaa cttccctcgc ttccagttct
     3061 gtaatgcgtt tgtttttagc ttctagttca tccagcagca ccagcacaac ctgcggtgtg
     3121 actttcatac gaaacgcaag caatttttgc ggcgttgcca cgacttttat tgcttctgcc
     3181 gcctcacgca gtgcctgata gtcaatcttg ctcactggtt gcctccttta cggatctgcg
     3241 ctgcgatgcg cgaaaaaaaa gcctcccgcg tatggctgtt aagagctggc gcgaacgccg
     3301 cgttaagaac ggcagcatca cagccgtcat cgatatagag cgcaattttt ttctccaggc
     3361 gcgctttggc ttcctgcaac tgcatacccc ggcacgcacg cgggatataa tcagcaattt
     3421 gagcgatagc tttttcgttc tgtttaaaca tgcttcacct cgataggctt gatggtgtct
     3481 aacagcagtc ggcggcgcgt attttctgca aaatggcggc gtccagtttc tttgtggtaa
     3541 aactcgtttt tgccaacgac ccacatccgc tctgtttggt gcaatttttt tacctgcggg
     3601 ccatctttgg tgatcacggt accggtatgg gtttttacga ttgtcatgcc acgacctctt
     3661 cgaatttcaa ctccaattgg tcaccccaga tttcacatga ttcggaacac gaaccagtat
     3721 caaaccgctt ggccagcacc atcgcctgat acaaacttct gtagtcgctg tcggcataca
     3781 ttctggcaat cccgtcaagc gtcaggtgac cacggtacat aacgtcttta cctgttctgc
     3841 gatgaccatc cctgacgtgt ttgcctgtaa ccagttcatt aaaaacccgc atcagaccag
     3901 gttcgtcttt acatgcaagc cccagctttt gcgttgactt tttgatgcaa aaaacacagt
     3961 tcccgaggtg ctccgggatt tgcaaatcaa aaggttgttt ttgccaccac cggataacat
     4021 ccgacttatc aaaatctgac agctcggcaa gataccggac gccaggtttc ggtttcagcc
     4081 tacggggttc gtccgcacga ataccgagcc atgtgatgta attccctcgc ccaaaatggt
     4141 catcgcaata tttcgtgaaa ggggtgagtt ttagcctgtc agtacagaat gcgccgccga
     4201 tgtatggcgt accgtacttt ttaaccatgt ccataaacgg tttaagcacc ggcattcgcg
     4261 tctgaatatc ctttggttcc cattccgtat aaccatttgg ctgcccaagc tctggattta
     4321 tatcgacctg caacacagtt agcggtatgc cccagaactt caaaacctcc cgaataaagc
     4381 ggtatgtcag cggatgttcg caaccggtat ccataaagat gtagcagacg ttattgccag
     4441 cctttctttg ttcttccatc aggtgaacaa gatatgcaga tgttctcccg ccagaaaaac
     4501 taactacatg agttatgcac atttgcgtaa ttccgataac tcgttgaagc gttccataaa
     4561 catcccgtag gcatggcccg gtgccagtgg aatcactttg aacatctctg ttgccgggat
     4621 accttccaat acaggccaga aagagccatc atcaagtccg agatcgcggc gttcggttgc
     4681 cagcatgatg agatcggcat atttcactgg cgtgctcata acaggaggta acccgtattt
     4741 ctcacggatt acggcgtcta ttttttcttc catccgttta tagtcaggaa gaagtcgttt
     4801 cagtggcgcg gggatgtcct ggcaatatgc ttctgttgca tcatgcatta acgcttcaaa
     4861 agcaaattcc tgcggcacca gctggctgca aagcaccgca tgctgggcga cactgtagaa
     4921 gtgtgaaaga tgtcctgcaa agcgacagat atttgaaagg gaaactgcga tatcgttaat
     4981 cacgatgtcg tctttattta tcttgtcata ataaaaatgc ttcccggaaa aagttttaat
     5041 aaatgacatt tcgttctcca ctttatatgc gctgcaccgc gctgaattct gctaaaagga
     5101 agctctcacc atccggtgat tattgagtta attacgtttc cataaatgcc cccgcagggg
     5161 catttgcagt aatgaaatca ggcggtgaaa gtaccaataa aggtttctac tttgctgtct
     5221 ttgaatttct caacaagcag atcacgaaat tcgttagcca tttcttcctg catcgcttcc
     5281 agctgaataa tgcgcagaac cagtacagga cgatcgccag tgataatgct gaggcgtaat
     5341 ttaaacggac gttctttcag gccttcaaac ggaacgcatt taaattcaaa tgccactggc
     5401 ataatgtctt tggtcttcgc ttcgacagac tccatcaggg agcgtttgcc gctgaagtca
     5461 ttgtcttcaa aatcagcggt ctggtttgct tcaattgtga ttttacggac tgccgcagcc
     5521 gctttggttg cctgaatggt gtcaccatta gcatcaaagc ccacaaggta gtcggcccag
     5581 tcttcaatcc attctgccag tgacttctgg gagttacgct cgccattaac agacaacaga
     5641 gcagaaaacg gtgctgtctt tttcagtttg agggtggcgg tgttatctgc gtgacctggt
     5701 tcatcaatag tacccaggtt aagcacactg acggc
//
LOCUS       DAZKHF020000270         5700 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2201S31241
            SAMN43267800-rid25106413.denovo.272, whole genome shotgun sequence.
ACCESSION   DAZKHF020000270 DAZKHF020000000
VERSION     DAZKHF020000270.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267800
            Sequence Read Archive: SRR30307263
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5700)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5700)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 5700)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350581.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350581.2
            Genome Coverage       :: 151x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:21:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,652
            CDSs (total)                      :: 5,557
            Genes (coding)                    :: 5,320
            CDSs (with protein)               :: 5,320
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 237
            CDSs (without protein)            :: 237
            Pseudo Genes (ambiguous residues) :: 0 of 237
            Pseudo Genes (frameshifted)       :: 102 of 237
            Pseudo Genes (incomplete)         :: 130 of 237
            Pseudo Genes (internal stop)      :: 52 of 237
            Pseudo Genes (multiple problems)  :: 41 of 237
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5700
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267800-rid25106413.denovo.272"
                     /serotype="O 26:H11"
                     /isolate="2201S31241"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-01"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            <1..354
                     /locus_tag="ACHOSC_RS20745"
                     /old_locus_tag="pgaptmp_004149"
     CDS             <1..354
                     /locus_tag="ACHOSC_RS20745"
                     /old_locus_tag="pgaptmp_004149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416627.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YehR family lipoprotein"
                     /protein_id="HGU4549970.1"
                     /translation="NGTEIAITYVYKGDKVLKQSSETKIQFASIGATTKEDAAKTLEP
                     LSAKYKNIAGVEEKLTYTDTYAQENVTIDMEKVDFKALQGISGINVSAEDAKKGITMA
                     QMELVMKAAGFKEVK"
     gene            complement(394..864)
                     /gene="yehS"
                     /locus_tag="ACHOSC_RS20750"
                     /old_locus_tag="pgaptmp_004150"
     CDS             complement(394..864)
                     /gene="yehS"
                     /locus_tag="ACHOSC_RS20750"
                     /old_locus_tag="pgaptmp_004150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1456 family protein"
                     /protein_id="HGU4549971.1"
                     /translation="MLSNDILRSVRYILKANNNDLVRILALGNVEATAEQIAVWLRKE
                     DEEGFQRCPDIFLSSFLNGLIYEKRGKDESAPALEPERRINNNIVLKKLRIAFSLKTD
                     DILAILTEQQFRVSMPEITAMMRAPDHKNFRECGDQFLRYFLRGLAARQHVKKG"
     gene            complement(911..1630)
                     /gene="btsR"
                     /locus_tag="ACHOSC_RS20755"
                     /old_locus_tag="pgaptmp_004151"
                     /gene_synonym="yehT"
     CDS             complement(911..1630)
                     /gene="btsR"
                     /locus_tag="ACHOSC_RS20755"
                     /old_locus_tag="pgaptmp_004151"
                     /gene_synonym="yehT"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310963.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator BtsR"
                     /protein_id="HGU4549972.1"
                     /translation="MIKVLIVDDEPLARENLRVFLQEQSDIEIVGECSNAVEGIGAVH
                     KLRPDVLFLDIQMPRISGLEMVGMLDPEHRPYIVFLTAFDEYAIKAFEEHAFDYLLKP
                     IDEARLEKTLARLRQERSKQDVSLLPENQQALKFIPCTGHSRIYLLQMKDVAFVSSRM
                     SGVYVTSHEGKEGFTELTLRTLESRTPLLRCHRQYLVNLAHLQEIRLEDNGQAELILR
                     NGLTVPVSRRYLKSLKEAIGL"
     gene            complement(1627..3312)
                     /gene="btsS"
                     /locus_tag="ACHOSC_RS20760"
                     /old_locus_tag="pgaptmp_004152"
     CDS             complement(1627..3312)
                     /gene="btsS"
                     /locus_tag="ACHOSC_RS20760"
                     /old_locus_tag="pgaptmp_004152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component regulatory system sensor histidine
                     kinase BtsS"
                     /protein_id="HGU4549973.1"
                     /translation="MYDFNLVLLLLQQMCVFLVIAWLMSKTPLFIPLMQVTVRLPHKF
                     LCYIVFSIFCIMGTWFGLHIDDSIANTRAIGAVMGGLLGGPVVGGLVGLTGGLHRYSM
                     GGMTALSCMISTIVEGLLGGLVHSILIRRGRTDKVFNPITAGAVTFVAEMVQMLIILA
                     IARPYEDAVRLVSNIAAPMMVTNTVGAALFMRILLDKRAMFEKYTSAFSATALKVAAS
                     TEGILRQGFNEVNSMKVAQVLYQELDIGAVAITDREKLLAFTGIGDDHHLPGKPISST
                     YTLKAIETGEVVYADGNEVPYRCSLHPQCKLGSTLVIPLRGENQRVMGTIKLYEAKNR
                     LFSSINRTLGEGIAQLLSAQILAGQYERQKAMLTQSEIKLLHAQVNPHFLFNALNTIK
                     AVIRRDSEQASQLVQYLSTFFRKNLKRPSEFVTLADEIEHVNAYLQIEKARFQSRLQV
                     NIAIPQELSQQQLPAFTLQPIVENAIKHGTSQLLDTGRVAISARREGQHLMLEIEDNA
                     GLYQPVTNASGLGMNLVDKRLRERFGDDYGISVACEPDSYTRITLRLPWRDEA"
     gene            3534..3599
                     /locus_tag="ACHOSC_RS20765"
                     /old_locus_tag="pgaptmp_004153"
                     /pseudo
     CDS             3534..3599
                     /locus_tag="ACHOSC_RS20765"
                     /old_locus_tag="pgaptmp_004153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416631.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            3827..4075
                     /locus_tag="ACHOSC_RS20770"
                     /old_locus_tag="pgaptmp_004154"
     CDS             3827..4075
                     /locus_tag="ACHOSC_RS20770"
                     /old_locus_tag="pgaptmp_004154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001217534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinI-like family protein"
                     /protein_id="HGU4549974.1"
                     /translation="MRVESCIAKEKISKMPKGSVPALQQEMLRRIRKRYDNAEIIIKS
                     ASNNGLTILRATDKESAHEFVQETLQEARETADDWFIH"
     gene            4697..5488
                     /locus_tag="ACHOSC_RS20775"
                     /old_locus_tag="pgaptmp_004155"
     CDS             4697..5488
                     /locus_tag="ACHOSC_RS20775"
                     /old_locus_tag="pgaptmp_004155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000509920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="avirulence protein"
                     /protein_id="HGU4549975.1"
                     /translation="MGHLERGLWKTETRWDGNDREQLGKEALGSEEPKPGSPFYGSRG
                     LKLSDSAHSAFSMMLCGSEGPFTKEQALSGFELAQTGQVLAGRLKIQERVKFRADNRI
                     DAQRNGTHSTRTPTGMDLSQDIGTIMRDKAGLPVMSGTSGSSSDATLATRYAAEHFGK
                     TWAAPGLSQAEGCKAISDLSHHYFRAEGSSPPQSMATGINKVRYDAGMEEKYVNTLDI
                     FTHSYPEIYAGVALTIAGAGGNDEQAMYNVTQEAARILHEAETKD"
BASE COUNT         1636 a         1387 c         1278 g         1399 t
ORIGIN      
        1 aacggcactg aaattgccat tacctatgtc tacaaaggtg acaaggtgct taagcaatct
       61 tctgaaacca aaattcaatt tgcttctatt ggtgcaacca ccaaagaaga cgctgccaag
      121 acacttgagc cgttaagcgc caaatacaaa aacatcgcgg gtgttgaaga aaaattaacc
      181 tatacagata cctacgcgca ggaaaacgtg actatcgata tggaaaaagt ggattttaaa
      241 gccctgcagg gtatttcagg aatcaacgtt tctgctgaag atgccaaaaa aggtatcact
      301 atggcgcaaa tggaactggt gatgaaagcc gctggtttta aagaagtgaa ataatcggtt
      361 ggcggtcatg ctctaaacat gaccgccaat tttttagcct tttttcacat gctggcgcgc
      421 tgccagtcca cgcagaaaat aacgtaaaaa ttgatcgccg cattcgcgga agtttttatg
      481 atccggtgcg cgcatcatcg ctgtaatttc cggcatcgaa acgcggaact gctgttcggt
      541 gaggatagcc agaatgtcat cggttttcag cgaaaacgcg atgcgtaatt ttttcagcac
      601 gatgttgtta ttaatgcgac gttccggctc cagtgccgga gcagactcat ccttgccgcg
      661 tttttcataa atcaggccat tgaggaatga cgacaaaaaa atgtccggac aacgctgaaa
      721 accctcttcg tcttctttac gtagccagac ggcgatctgt tccgcggtgg cttcgacatt
      781 acccagcgcc agaatacgca ccaggtcatt attattggct ttcaaaatgt agcgcacgct
      841 gcgcagaata tcgttactta gcatgaggcc ttcaggtgtt gatgaggcaa aaagccattt
      901 tagcagtctt ttacaggcca atcgcctctt ttaagctttt cagataacgg cggctgaccg
      961 gcacggttaa gccattacgc aaaatcaact cggcctggcc gttatcttcc agacgaatct
     1021 cctgtaaatg cgcgaggtta accagatact gacgatggca gcgcagtagt ggtgtacgac
     1081 tttccagggt acgtaatgtc aattcggtaa agccctcttt cccttcgtgg ctggtaacgt
     1141 agacaccgct catccgactg ctgacaaatg ccacatcttt catttgcagc aaataaatcc
     1201 gactatgccc cgtacaaggg ataaatttca gcgcctgttg attttccggt aacagcgaaa
     1261 catcctgctt gctgcgctcc tgacgcaatc gcgccagcgt tttctccagt cgcgcttcat
     1321 caattggctt cagcagataa tcaaaggcat gttcttcaaa ggctttaata gcgtattcgt
     1381 caaacgcagt gagaaaaaca atatacgggc gatgttccgg gtcaagcatc cccaccattt
     1441 ccagaccact gatgcgcggc atctggatat cgagaaacag cacatccggg cgcagtttat
     1501 gcaccgcgcc gatcccttcc acggcgtttg aacactctcc aacgatttca atatcgctct
     1561 gctcctgcaa aaatacgcgc aggttctccc gtgctaacgg ttcatcatcg acaattaaga
     1621 ctttaatcat gcctcgtccc tccatggtag tcgtaacgtt attcgggtgt aactatcagg
     1681 ctcacaggcg acgcttattc catagtcatc gccaaaccgt tcacgtaaac gcttatccac
     1741 cagattcatc cccagcccac tggcattggt taccggttga tacaaaccgg cattgtcttc
     1801 gatctccagc atcaaatgtt gcccctcacg tcgggcgctg attgccactc gccctgtatc
     1861 cagcagttgt gatgtcccat gtttaatggc gttttccact atcggttgca gggtaaacgc
     1921 gggcaattgc tgctgggata attcttgcgg aatagcaatg ttgacctgca accgcgactg
     1981 gaagcgcgcc ttttcaattt gcagataagc gttcacatgt tcaatttcgt cggcgagagt
     2041 aacaaactcc gaaggccgct ttaagttttt gcggaaaaaa gtggaaagat actgcaccag
     2101 ctggctggcc tgttcgctgt cgcggcggat caccgcttta atggtgttaa gcgcattaaa
     2161 caaaaaatga ggattcacct gggcgtgaag cagtttgatt tctgactggg tgagcatcgc
     2221 tttttgccgc tcatattgcc cggcaagaat ctgcgccgaa agcagttgcg caatcccctc
     2281 gcccagcgtg cggtttattg aactgaataa acggtttttg gcttcataca atttgatggt
     2341 gcccatcacg cgctgatttt cgccacgcaa cggaattacc agcgtcgacc ccagtttgca
     2401 ttgcggatgc aaagagcaac gataaggtac ttcgttgcca tcagcgtaga ccacttcacc
     2461 ggtttcaatc gcttttaagg tgtaagtcga agaaatcggt ttgccgggta aatggtggtc
     2521 gtcaccaatt ccggtaaagg ccagcaattt ctctcgatcg gtaatcgcga ctgcaccaat
     2581 atccagctcc tgatacagca cctgagccac tttcatgctg ttcacttcgt taaacccctg
     2641 tcgcaaaatg ccttccgtcg aggctgccac tttcagcgca gtggcagaaa aagcagaagt
     2701 gtatttttca aacatcgcgc gtttatcgag caatatacgc ataaacagcg ccgcgccgac
     2761 ggtattggtg accatcattg gtgcagcaat attactcacc agacgcaccg catcttcata
     2821 aggtcgggcg atcgcaagga tgatcagcat ttgcaccatt tcagcgacga acgtgacggc
     2881 accggcggta atggggttaa agactttatc agtgcgcccg cgacggatca ggatgctgtg
     2941 taccaggccg ccgagcaatc cttcaacgat ggttgagatc atgcaactta gcgcggtcat
     3001 gccccccatc gaatatcgat gtaagccgcc ggtcagacca accagcccac cgacgaccgg
     3061 accgccgagt aagccgccca ttaccgcgcc tatcgcacgg gtattggcaa tagaatcgtc
     3121 aatgtgcaac ccaaaccagg tgcccatgat gcagaagatg gaaaagacga tgtagcagag
     3181 aaatttatgc ggcagacgaa ccgtgacctg cattaacggt atgaataatg gcgttttact
     3241 cattaaccac gcaatgacta aaaaaacgca catctgctga agcagcagca acaccagatt
     3301 aaaatcgtac atacccgcaa accacacttc cctttaaaac gcgtaacata cattgcctgc
     3361 gtttaacttt ctttgaactc ttgcagaaaa atgagaattc gtgagtacga tcactcaaaa
     3421 tcgcctggca aaaataaaat caccctatag atgcacaaaa aacgggcaaa actacctggt
     3481 tcgcaaaact gcgtctaaag ttaaaccggg acctcgcgag caagggtgag acgatggcgc
     3541 tttacacaat tggtgaagtg gcgttgcttt gtgatattaa ccctgtcacg ttacgcgcgt
     3601 gacgcaacat ttttaaaaat caacgaagat cgtccaggag cgccgatttt caagggttgg
     3661 acagataatt cgttttcgat gtcgacgaac ttcgacggat ttcgcctata aaaagtgtcc
     3721 catacatgcc ccattgatgc cccatagaca atatcagcaa ttgacactgg ttatctatac
     3781 aactaaaaat aacacctgta cataaccaca gttacaggag ggtgttatgc gtgttgagag
     3841 ctgtatcgct aaagaaaaaa tcagcaaaat gccaaaaggt tctgttcctg ccctgcaaca
     3901 ggaaatgctg cgccgtatca ggaaacgata tgacaatgca gaaatcatca tcaaatctgc
     3961 cagtaataat ggactcacta ttttaagagc cacagataaa gagtccgcac acgaatttgt
     4021 acaggaaaca cttcaggaag ccagggaaac agcggacgac tggtttattc actgactaaa
     4081 atcccgtcca catgccggga atttcctatg tccgaagaaa atggtgtggt taactaagca
     4141 aaagtgtccc tttatcggta tagcaactca ttttgttaac atacggttgc aaaattgatg
     4201 acaagtgttt attttagcca caagaaatgt atgcaaacca gaattgggaa atgcatttat
     4261 aaaataagga atagcagata tgtttgcagc aaagcccgaa ttaagtcaaa gatgtatttc
     4321 tggactcctc acaactgggc caaatgatga aaacgtaaat aataaaccgt caagtgatat
     4381 gccgaacaga atcaaactag ttgtaagcga ttcacaaatc ggtatatata caactgacag
     4441 taatactctg aaggatggat tgtgtagcca agcccacata ggaactcaac cttatcggcc
     4501 aggtgctgac tcgccgataa ctgtgagatc attcctacaa atcacacttt ccaacaataa
     4561 ttacaaatca ttagagcatc tgcaaacatg agcagagaat tatctgaggt atcgcaaagc
     4621 agaagagaat attctgcgtt cgaccgttga ggggctaacc aatccagaat ctcctgtatt
     4681 caaacaaaca gcatggatgg ggcatctgga gcgtggcctt tggaaaacgg aaacacgctg
     4741 ggatggaaac gaccgcgagc agctaggcaa agaggctctt ggctcagagg agccaaaacc
     4801 tggttcacca ttttacggta gtcgtggact aaaattatcc gacagtgcgc attctgcctt
     4861 ctcaatgatg ctgtgcggtt ctgaaggtcc attcacaaaa gaacaagcac tctctggttt
     4921 cgagcttgct caaactggcc aagtgctggc tggaaggcta aagattcagg agcgagttaa
     4981 attccgcgca gacaaccgca ttgatgctca gcgtaacggt acacactcaa cccgcacacc
     5041 aacaggcatg gatctttctc aagatatcgg cacaattatg cgtgataaag ctgggctacc
     5101 ggtcatgagc ggaacatccg gttcctcctc tgatgccact cttgcaacac gttacgctgc
     5161 cgagcatttt ggtaagacat gggccgctcc aggcttaagc caagcagaag gatgcaaagc
     5221 tatctcggat ctgtcacatc actattttcg tgcagaaggc tccagtccac cacaatcaat
     5281 ggcaacggga ataaataagg ttcgttatga tgcaggaatg gaagagaaat atgtaaatac
     5341 actggatata tttacacata gctacccaga aatttatgct ggggttgcat tgacgattgc
     5401 tggggctggc ggtaatgatg agcaggcaat gtataatgtt acgcaagaag ccgctcgtat
     5461 tctgcacgaa gctgaaacta aagattgaac attctcaata aagagctgcg ccaggcagca
     5521 catattcagc attgcagtaa gatatgcatg cccagccccc tgtaaaccta taaatatttc
     5581 aaatcatatt gattatctta tagccatatt ttttatgaca ccctgtttca gtgaacatga
     5641 agacactcac cttgttcctg atattcatta gcatcatcct gattatttaa cttctctatt
//
LOCUS       DAZKHF020000271         5686 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2201S31241
            SAMN43267800-rid25106413.denovo.273, whole genome shotgun sequence.
ACCESSION   DAZKHF020000271 DAZKHF020000000
VERSION     DAZKHF020000271.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267800
            Sequence Read Archive: SRR30307263
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5686)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5686)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 5686)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350581.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350581.2
            Genome Coverage       :: 151x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:21:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,652
            CDSs (total)                      :: 5,557
            Genes (coding)                    :: 5,320
            CDSs (with protein)               :: 5,320
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 237
            CDSs (without protein)            :: 237
            Pseudo Genes (ambiguous residues) :: 0 of 237
            Pseudo Genes (frameshifted)       :: 102 of 237
            Pseudo Genes (incomplete)         :: 130 of 237
            Pseudo Genes (internal stop)      :: 52 of 237
            Pseudo Genes (multiple problems)  :: 41 of 237
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5686
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267800-rid25106413.denovo.273"
                     /serotype="O 26:H11"
                     /isolate="2201S31241"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-01"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            166..876
                     /locus_tag="ACHOSC_RS20780"
                     /old_locus_tag="pgaptmp_004156"
     CDS             166..876
                     /locus_tag="ACHOSC_RS20780"
                     /old_locus_tag="pgaptmp_004156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbria/pilus periplasmic chaperone"
                     /protein_id="HGU4549976.1"
                     /translation="MQTTRTPYSISFMATVLLLLLFACHSTVANAAVALGATRVIYPA
                     NQKQVLLPVTNNDPASVYLIQSWIENAGDQKDTQFVITPPLFSMQGKKENTLRIINAT
                     NHQLPGDRESLFWVNVKAIPAMEKDQKNENTLQLAIISRIKMFYRPTNLAMAPEEAPA
                     MLRFRRSGSKLTLINPTPYFITVTNMKAGNSNLPNTMVPPKGEVSVDITHAATGDISF
                     QTINDYGALTPRIKATMQ"
     gene            918..3568
                     /locus_tag="ACHOSC_RS20785"
                     /old_locus_tag="pgaptmp_004157"
                     /pseudo
     CDS             918..3568
                     /locus_tag="ACHOSC_RS20785"
                     /old_locus_tag="pgaptmp_004157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310137.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0015473 - fimbrial usher porin activity
                     [Evidence IEA];
                     GO_process: GO:0009297 - pilus assembly [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="fimbria/pilus outer membrane usher protein"
     gene            3582..4112
                     /locus_tag="ACHOSC_RS20790"
                     /old_locus_tag="pgaptmp_004158"
     CDS             3582..4112
                     /locus_tag="ACHOSC_RS20790"
                     /old_locus_tag="pgaptmp_004158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HGU4549977.1"
                     /translation="MKYNNIIFLGLCLGLTTYSALSADSVIKISGRVLDYGCTVSSDS
                     LNFTVDLQKNSARQFPTTGSTSPAVPFQITLSECSKGTTGVRVAFNGIEDAENNTLLK
                     LDEGSNTASGLGIEILDANMRPVKLNDLHAGMQWIPLVPEQNNILPYSARLKSTQKSV
                     NPGLVRASATFTLEFQ"
     gene            4125..4628
                     /locus_tag="ACHOSC_RS20795"
                     /old_locus_tag="pgaptmp_004159"
     CDS             4125..4628
                     /locus_tag="ACHOSC_RS20795"
                     /old_locus_tag="pgaptmp_004159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HGU4549978.1"
                     /translation="MKRLHKRFLLATFCALFTATLQAADVTITVNGRVVAKPCTIQTK
                     EANVNLGDLYTRNLQQPGSASGWHNITLSLTDCPVETSAVTAIVTGSTDNTGYYKNEG
                     TAENIQIELRDDQDAALKNGDSKTVIVDEITRNAQFPLKARAITVNGNASQGTIEALI
                     NVIYTWQ"
     gene            4687..5601
                     /locus_tag="ACHOSC_RS20800"
                     /old_locus_tag="pgaptmp_004160"
     CDS             4687..5601
                     /locus_tag="ACHOSC_RS20800"
                     /old_locus_tag="pgaptmp_004160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HGU4549979.1"
                     /translation="MGKTISIKVLFGIYLLLMAGKVFAFSCNVDGGSSIGAGTTSVYV
                     NLDPVIQPGQNLVVDLSQHISCWNDYGGWYDTDHINLVQGSAFAGSLQSYKGSLYWNN
                     VTYPFPLTTNTNVLDIGDKTPMPLPLKLYITPVGAAGGVVIKAGEVIARIHMYKIATL
                     GSGNPRNFTWNIISNNNVVMPTGGCTVDSRNVTVDLPDFPGSAEIPLGVYCSSEQKLS
                     FYLSGATTDSSRQVFANTAPDATKASGVGVTLMRNGKILATGENVSLGTVNKSKVPLG
                     LSATYGQTGNKVSAGTVQSVIGVTFIYE"
BASE COUNT         1637 a         1232 c         1267 g         1550 t
ORIGIN      
        1 tatttacttt gtagcaaggt tttcctatta aaagagaacc aacatttatt acaaacgcat
       61 taagtctgga aacccggcga ctcgccaatg tattagcaac attaatcgat tgtctgatgt
      121 aacggttttc ccgcttatcc tcttaacaca tggagaatgc atcagatgca aactacgcgt
      181 accccctact ctatttcctt tatggcgaca gtgttacttt tattgctctt tgcttgccat
      241 agcaccgttg ccaacgcagc tgtcgcactg ggtgcgacgc gggtaattta tccggcaaac
      301 cagaaacagg ttttattgcc tgtcacgaat aatgatcctg ccagtgtcta tctcattcag
      361 tcatggatag aaaatgcagg tgaccaaaaa gacacccagt ttgtcattac cccaccgctt
      421 ttttccatgc aaggtaaaaa agagaatacg ctgcggataa tcaacgctac taatcatcag
      481 ttaccaggcg accgtgaaag cctgttctgg gtgaatgtta aagctattcc tgctatggaa
      541 aaagaccaga agaatgagaa tacattacag ctggcaatta ttagccgcat aaaaatgttt
      601 tatcgcccca cgaacctggc aatggcacct gaagaagcgc ctgcaatgct tcggtttcgt
      661 cgcagcggta gcaaactcac gcttattaat ccaacgccct atttcattac cgtaacaaat
      721 atgaaagcag gaaatagcaa tctcccaaac accatggtgc cgccaaaagg tgaagtatcg
      781 gtggatatca cgcatgctgc aaccggggat atcagttttc agacgatcaa tgattacggt
      841 gcattaactc cgcgtattaa ggcaaccatg cagtaagcca gtgacctaat taataataaa
      901 ctcaatcgga attaataatg acagcctttc gtgccgcatt taaggcatat cgtatgcatc
      961 aggtgttaat aatgcctcgc tttgcacggc ttaccattgc ccttggctta gcaactgcgg
     1021 tatttcccgt tgacgcagag tattatttta atccacggtt tttaagcaat gatcttgctg
     1081 aatctgtcga tttatccgcc tttactaagg gacgcgaggc accacctggt acatatcggg
     1141 tcgacattta tttgaacgac gaatttatga ctagtcggga tattactttt attgcagatg
     1201 ataataacgc agaactcatc ccatgcctga gtacagatct tcttgtcagt cttggtataa
     1261 aaaaatagcg ttattagaca ataaagaaca ttcggcagaa aaacatgtgc cggacaacag
     1321 cgcatgcaca ccacttcagg atcgtttggc cgacgcatca actgagtttg atgttggtca
     1381 gcaacatttg tcactctccg ttccgcaaat ttatgttggc agaatggctc gcggctatgt
     1441 ttctcctgat ctgtgggaag aaggaataaa tgctgggctt ctaaactata gttttaatgg
     1501 taattctatt aataatcgta gtaaccataa tgcaggaaaa tccaactatg catatttgaa
     1561 tttacagagt ggcatcaaca ttggtagttg gcgactgcgc gataactcaa cgtggagtta
     1621 taacagtggg agtagcaatt catctgacag caataaatgg cagcatatca atacgtcggc
     1681 tgaacgtgac attattccct tacgctcacg gttaacggta ggtgatagtt ataccgatgg
     1741 cgatattttt gatagtgtga actttcgtgg cctcaaaata aattcaacag aagcgatgtt
     1801 gcccgatagc caacatggtt tcgctccggt gattcatggt attgcccgtg gaaccgcaca
     1861 agtgagtgta aaacagaacg gatacgatgt ttatcagact actgttccac ccggcccttt
     1921 tactattgat gatatcaact ctgcggccaa tggtggtgat ctgcaagtaa ccataaaaga
     1981 ggcagacggc agtattcaga cattatatgt tccttattcg tctgttccgg ttctccaacg
     2041 tgctggatat acgcgttatg cgcttgccat gggggaatat cgtagtggaa ataacctgca
     2101 aagctctccc aagttcatac aaggtagctt gatgcatggt ctggaaggaa actggacacc
     2161 ttatggcgga atgcaaattg cagaagatta tcaggccttc aaccttggta ttgggaaaga
     2221 tttaggactt tttggtgcct tttctttcga tatcacgcag gccaatacga cacttgcaga
     2281 tggcacccgt cacagtgggc aatcggttaa atccgtctac agcaaatcct tctaccagac
     2341 aggaaccaat atccaggtcg caggatatcg ctattctacg caaggttttt ataacttatc
     2401 cgacagtgcc tacagtcgaa tgagtggtta caccgtcaag cctcctaccg gagacaccaa
     2461 tgagcagaca caatttattg attattttaa tctgttctac agtaagcgtg gtcaggaaca
     2521 aataagcatc tctcagcagc ttggaaatta cggtacgaca tttttcagtg ccagtcgcca
     2581 aagttactgg aacacgtcac gcagcgacca gcaaatatca tttggattaa atgtgccgtt
     2641 tggtgatatt acgacttcgc tgaattacag ctattccaat aatatatggc aaaacgatcg
     2701 ggatcattta ctcgctttta cgcttaatgt tcccttcagt cattggatgc gtacagacag
     2761 tcagtcggca tttcgtaatt caaacgccag ttacagtatg tcaaacgatt tgaaaggcgg
     2821 catgaccaat ctatcggggg tttatggcac tctgctgccg gataataacc tgaattatag
     2881 cgttcaggtc ggtaacaccc acggaggtaa tacatcgtct ggcaccagtg gttacagttc
     2941 tcttaattat cgtggagctt atggtaatac taatgtcggt tacagtcgga gtggtgacag
     3001 cagccagatt tattacggaa tgagtggtgg gattattgct catgctgatg gcatcacctt
     3061 tggacagccg ctgggcgaca caatggttct ggttaaggct cctggtgctg ataatgtcaa
     3121 aatagagaac cagaccggaa ttcataccga ctggcgtggc tatgccatat taccatttgc
     3181 gacagaatat agagaaaacc gtgttgctct taacgcgaat tcccttgcag ataatgttga
     3241 actggatgaa accgtgatca ctgtcatccc aactcacggt gctattgcca gagcaacatt
     3301 taatgcacaa atcggcggga aagtattaat gacgttgaag tacggtaata agagtgttcc
     3361 attcggtgca attgtcacac acggagagaa taaaaatggc agcattgtcg cagaaaacgg
     3421 tcaggtttat ctgactggac ttccacagtc agggaaatta caggtttcat ggggcaaaga
     3481 taaaaactca aactgtattg tcgagtacaa gcttcctgaa gtttctcctg gtaccttact
     3541 gaaccagcag acagcaatct gtcgctaagg aacaatagat tatgaaatac aataacatta
     3601 ttttcctcgg tttatgtctg gggttaacca cctattctgc tttatccgca gatagcgtta
     3661 ttaaaattag cgggcgcgtc ctcgattatg gctgcacagt ctcatcggat tcgcttaatt
     3721 ttaccgtaga tctccaaaaa aacagtgcca gacaatttcc aacgaccggt agcacaagtc
     3781 cagccgtccc ttttcagatt acgttaagtg aatgcagcaa agggacaacg ggggttcggg
     3841 ttgcatttaa cggtattgag gatgcagaaa ataatacttt gttgaaactg gatgaaggaa
     3901 gcaatacggc ctccggtttg ggtatagaaa tattggacgc aaatatgcgt ccggtgaaac
     3961 tgaatgacct tcatgccggg atgcagtgga tcccactggt accagaacag aacaatattt
     4021 tgccttactc cgctcgtctg aagtcaactc agaagtccgt caatccggga ctggtgaggg
     4081 cttcggcaac ctttaccctt gaatttcaat aatcagagtc agatatgaaa agacttcaca
     4141 agaggttcct gttagctacg ttttgcgcgt tattcacagc aactctccag gccgccgatg
     4201 tcactatcac tgttaatggt cgggtagtcg ctaaaccctg cactattcaa accaaagaag
     4261 ctaacgttaa tctcggggat ctttatacgc gcaatctgca acaacctggt tctgcatctg
     4321 gctggcacaa tattactttg tcattaaccg attgtccggt tgaaacaagt gcagtgacgg
     4381 caatcgtgac aggttcaact gacaatacgg gttattacaa aaatgaaggt actgccgaaa
     4441 atattcagat agagctgagg gatgaccagg atgctgcgtt aaaaaatggc gatagcaaaa
     4501 cggttattgt tgatgagatc actcgtaatg cacagtttcc acttaaggca agagctatca
     4561 cggtgaatgg aaacgcaagc cagggaacga tcgaggcgct aatcaatgtg atctacacct
     4621 ggcaataacg tcgattcgat tttcttagcc ttctttttaa atcaattttt aaccttagga
     4681 tatatcatgg gtaaaacaat cagtataaag gtcctgttcg gtatttatct tctattaatg
     4741 gcaggcaaag tttttgcctt cagttgtaat gtcgatggcg ggtcaagtat tggagcaggt
     4801 acaacctctg tttatgttaa tctcgaccct gtaatacagc cgggccagaa tctggttgta
     4861 gacttgtctc agcatataag ttgctggaat gattacggcg gctggtacga cactgatcat
     4921 ataaacctgg tacaaggctc agctttcgca ggctccctac agtcatataa aggatcgctg
     4981 tactggaata atgtgaccta ccccttcccg ttgacgacaa ataccaacgt attggatatt
     5041 ggtgataaga caccgatgcc attaccgtta aaactctata ttaccccagt tggggcggcc
     5101 ggtggagttg tcataaaagc gggggaagtc attgcccgta tacatatgta taaaattgca
     5161 acattaggaa gcggaaatcc gcgtaatttt acgtggaata ttatttcaaa taacaatgtt
     5221 gttatgccaa caggcggctg caccgttgat agtcgtaatg tcaccgtgga tctcccggac
     5281 tttcctggaa gcgcagaaat accccttggc gtatattgct caagcgaaca aaagctctca
     5341 ttttatcttt cgggtgccac cactgacagt agccgacaag tgtttgctaa cacagcacct
     5401 gatgcaacaa aagcatcggg agtgggtgtc actctaatgc gtaatggaaa aatactggct
     5461 acgggagaaa atgtctccct ggggacagta aataaaagca aagtacctct gggtttaagc
     5521 gcaacctatg gacaaacagg aaacaaggtt tcagccggaa ccgtccagtc agtcattggt
     5581 gtcactttta tctatgaata attgtgattt tgaccttagt ctatgcgatc cattcacgaa
     5641 aattgcgcgg ataattattt tgtgaaggct attagcctac acctgt
//
LOCUS       DAZKHF020000272         5624 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2201S31241
            SAMN43267800-rid25106413.denovo.274, whole genome shotgun sequence.
ACCESSION   DAZKHF020000272 DAZKHF020000000
VERSION     DAZKHF020000272.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267800
            Sequence Read Archive: SRR30307263
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5624)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5624)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 5624)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350581.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350581.2
            Genome Coverage       :: 151x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:21:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,652
            CDSs (total)                      :: 5,557
            Genes (coding)                    :: 5,320
            CDSs (with protein)               :: 5,320
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 237
            CDSs (without protein)            :: 237
            Pseudo Genes (ambiguous residues) :: 0 of 237
            Pseudo Genes (frameshifted)       :: 102 of 237
            Pseudo Genes (incomplete)         :: 130 of 237
            Pseudo Genes (internal stop)      :: 52 of 237
            Pseudo Genes (multiple problems)  :: 41 of 237
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5624
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267800-rid25106413.denovo.274"
                     /serotype="O 26:H11"
                     /isolate="2201S31241"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-01"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            complement(<1..282)
                     /locus_tag="ACHOSC_RS20805"
                     /old_locus_tag="pgaptmp_004161"
     CDS             complement(<1..282)
                     /locus_tag="ACHOSC_RS20805"
                     /old_locus_tag="pgaptmp_004161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HGU4549980.1"
                     /translation="MILHAQAKHGKPGLPWLVFLHGFSGDCHEWQEVGEAFADYSRLY
                     VDLPGHGGSAAISVDGFDDVTDLLRKTLVSYNILDFWLVGYSLGGRVAMM"
     gene            complement(279..1949)
                     /gene="menD"
                     /locus_tag="ACHOSC_RS20810"
                     /old_locus_tag="pgaptmp_004162"
     CDS             complement(279..1949)
                     /gene="menD"
                     /locus_tag="ACHOSC_RS20810"
                     /old_locus_tag="pgaptmp_004162"
                     /EC_number="2.2.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_function: GO:0070204 -
                     2-succinyl-5-enolpyruvyl-6-hydroxy-3-cyclohexene-1-
                     carboxylic-acid synthase activity [Evidence IEA];
                     GO_process: GO:0009234 - menaquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-succinyl-5-enolpyruvyl-6-hydroxy-3-
                     cyclohexene-1-carboxylic-acid synthase"
                     /protein_id="HGU4549981.1"
                     /translation="MSVSAFNRRWAAVILEALTRHGVRHICIAPGSRSTPLTLAAAEN
                     SAFIHHTHFDERGLGHLALGLAKVSKQPVAVIVTSGTAVANLYPALIEAGLTGEKLIL
                     LTADRPPELIDCGANQAIRQPGMFASHPTHSISLPRPTQDIPARWLVSTIDHALGTLH
                     AGGVHINCPFAEPLYGEMDDTGLSWQQRLGDWWQDDKPWLREAPRLESEKQRDWFFWR
                     QKRGVVVAGRMSAEEGKKVALWAQTLGWPLIGDVLSQTGQPLPCADLWLGNAKATSEL
                     QQAQIVVQLGSSLTGKRLLQWQASCEPEEYWIVDDIEGRLDPAHHRGRRLIANIADWL
                     ELHPAEKRQPWCVEIPRLAEQAMQAVIARRDAFGEAQLAHRISDYLPEQGQLFVGNSL
                     VVRLIDALSQLPAGYPVYSNRGASGIDGLLSTAAGVQRASGKPTLAIVGDLSALYDLN
                     ALALLRQVSAPLVLIVVNNNGGQIFSLLPTPQSERERFYLMPQNVHFEHAAAMFELKY
                     HRPQNWQELETAFADAWRTPTTTVIEMVVNDTDGAQTLQQLLAQVSHL"
     gene            complement(2038..3333)
                     /gene="menF"
                     /locus_tag="ACHOSC_RS20815"
                     /old_locus_tag="pgaptmp_004163"
     CDS             complement(2038..3333)
                     /gene="menF"
                     /locus_tag="ACHOSC_RS20815"
                     /old_locus_tag="pgaptmp_004163"
                     /EC_number="5.4.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416768.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008909 - isochorismate synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isochorismate synthase MenF"
                     /protein_id="HGU4549982.1"
                     /translation="MQSLTTALENLLRHLSQEIPATPGIRVIDIPFPLKDAFDALSWL
                     ASQQTYPQFYWQQRNGDEEAAVLGAITRFTSLDQAQRFLRQHPEHADLRIWGLNAFDP
                     SQGNLLLPRLEWRRCGGKATLRLTLFSESSLQHDAIQAKEFIATLVSIKPLPGLHLTT
                     TREQHWPDKTGWTQLIELATKTIAEGELDKVVLARATDLHFASPVNAAAMMAASRRLN
                     LNCYHFYMAFDGENAFLGSSPERLWRRRDKALRTEALAGTVANNPDDKQAQQLGEWLM
                     ADDKNQRENMLVVEDICQRLQADTQTLDVLPPQVLRLRKVQHLRRCIWTSLNKADDVI
                     CLHQLQPTAAVAGLPRDLARQFIARHEPFTREWYAGSAGYLSLQQSEFCVSLRSAKIS
                     GNVVRLYAGAGIVRGSDPEQEWQEIDNKAAGLRTLLQME"
     gene            complement(3412..3717)
                     /gene="elaB"
                     /locus_tag="ACHOSC_RS20820"
                     /old_locus_tag="pgaptmp_004164"
     CDS             complement(3412..3717)
                     /gene="elaB"
                     /locus_tag="ACHOSC_RS20820"
                     /old_locus_tag="pgaptmp_004164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stress response protein ElaB"
                     /protein_id="HGU4549983.1"
                     /translation="MSNQFGDTRIDDDLTLLSETLEEVLRSSGDPADQKYVELKARAE
                     KALDDVKKRVSQASDSYYYRAKQAVYRADDYVHEKPWQGIGVGAAVGLVLGLLLARR"
     gene            complement(3772..4233)
                     /locus_tag="ACHOSC_RS20825"
                     /old_locus_tag="pgaptmp_004165"
     CDS             complement(3772..4233)
                     /locus_tag="ACHOSC_RS20825"
                     /old_locus_tag="pgaptmp_004165"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HGU4549984.1"
                     /translation="MIEWQDLHHSELSVSQLYALLQLRCAVFVVEQNCPYQDIDGDDL
                     TGDNRHILGWKNDELVAYARILKSDDDLEPVVIGRVIVSEALRGEKVGQQLMSKTLET
                     CTHHWPDKPVYLGAQAHLQNFYQSFGFIPVTEVYEEDGIPHIGMAREVIQA"
     gene            4298..5215
                     /gene="rbn"
                     /locus_tag="ACHOSC_RS20830"
                     /old_locus_tag="pgaptmp_004166"
     CDS             4298..5215
                     /gene="rbn"
                     /locus_tag="ACHOSC_RS20830"
                     /old_locus_tag="pgaptmp_004166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416771.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016891 - RNA endonuclease activity,
                     producing 5'-phosphomonoesters [Evidence IEA];
                     GO_process: GO:0042780 - tRNA 3'-end processing [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease BN"
                     /protein_id="HGU4549985.1"
                     /translation="MELIFLGTSAGVPTRTRNVTAILLNLQHPTQSGLWLFDCGEGTQ
                     HQLLHTAFNPGKLDKIFISHLHGDHLFGLPGLLCSRSMSGIIQPLTIYGPQGIREFVE
                     TALRISGSWTDYPLEIVEIGAGEILDDGLRKVTAYPLEHPLECYGYRIEEHDKPGALN
                     AQALKAAGVPPGPLFQELKAGKTITLEDGRQINGADYLAAPVPGKALAIFGDTGPCDA
                     ALDLAKGVDVMVHEATLDITMEAKANSRGHSSTRQAATLAREAGVGKLIITHVSSRYD
                     DKGCQHLLRECRSIFPATELANDFTVFNV"
     gene            5403..>5624
                     /locus_tag="ACHOSC_RS20835"
                     /old_locus_tag="pgaptmp_004167"
     CDS             5403..>5624
                     /locus_tag="ACHOSC_RS20835"
                     /old_locus_tag="pgaptmp_004167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001697257.1"
                     /note="ElaD; specific cysteine protease which targets
                     ubiquitin and ubiquitin-like proteins covalently bound to
                     target proteins; SseL in Salmonella is required for
                     macrophage killing and virulence, and it is secreted by
                     the salmonella pathogenicity island 2 type III secretion
                     system; Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deubiquitinase"
                     /protein_id="HGU4549986.1"
                     /translation="MMVTVVSNYCQLSQTQLSQTFAEKFTVTEELLQSLKKTALSGDE
                     ESIELLHNIALGYDEFGKKAEDILYHIVRN"
BASE COUNT         1345 a         1540 c         1386 g         1353 t
ORIGIN      
        1 catcatcgcc acccgtccgc caagcgagta ccccaccagc cagaagtcaa ggatgttgta
       61 actaaccaag gttttacgca gtaagtcggt gacatcatca aatccatcga cgctaatcgc
      121 cgccgaacca ccatgacctg ggagatcaac atacaaccgt gagtagtcgg caaacgcctc
      181 gcccacttct tgccattcgt ggcaatcgcc ggaaaaaccg tgcaaaaaca ccagccaggg
      241 taaacctggt tttccgtgtt ttgcctgcgc gtgcaggatc ataaatggct tacctgcgcc
      301 agaagttgct ggagcgtttg cgcaccatcg gtgtcgttaa ccaccatttc aatcaccgtg
      361 gtggttggcg tgcgccaggc gtcggcaaat gccgtttcaa gttcctgcca gttttgcgga
      421 cgatgatatt tcagctcgaa catcgcggcg gcgtgctcaa aatggacgtt ttgcggcatc
      481 agatagaaac gctcacgctc gctttgcggc gttggcaaca gcgagaaaat ttgcccgccg
      541 ttgttgttca ccacaattaa taccagcggc gcagaaacct gacgcaataa cgccagcgcg
      601 ttgagatcgt aaagtgcgga gagatcgccc acaatcgcca gcgtcggttt gccgcttgcc
      661 cgctgaacgc ctgcggcggt cgaaagcagt ccgtcgatac cgctggcccc tcggttgctg
      721 tacaccgggt aacctgccgg aagttgcgaa agcgcatcaa tcagacgtac caccaggctg
      781 ttaccaacaa acaattgccc ctgttcaggc agatagtcgc tgatgcgatg cgccagttgc
      841 gcttcgccaa acgcatcacg gcgggcaata accgcctgca ttgcctgttc cgccaggcgc
      901 gggatttcaa cgcaccaggg ctggcgtttt tctgccggat gcagctccag ccagtcggca
      961 atattggcaa ttaagcgacg tccgcgatgg tgtgccggat caagtcgccc ttcaatgtca
     1021 tcaacaatcc agtactcttc gggttcacag cttgcctgcc attgcaggag ccgtttcccc
     1081 gtcaggctgc ttcccagttg caccacaatt tgcgcctgct gcagctcgct ggtcgctttg
     1141 gcattgccta accaaagatc ggcacacggc agcggctgcc cggtttgtga cagcacatcg
     1201 ccaatcagcg gccagccaag agtttgcgcc cacagggcaa cttttttgcc ctcttccgca
     1261 ctcatgcgcc cggcaaccac cacgccgcgc ttttgtcgcc agaagaacca gtcgcgctgt
     1321 ttttcacttt ccagacgagg cgcttcacgc agccacggtt tgtcgtcctg ccaccagtca
     1381 cccagacgct gttgccagct aagcccggta tcgtccattt cgccatacag cggttcagca
     1441 aacgggcagt tgatatggac tccccccgca tgaagcgtac cgagagcgtg gtcgatggta
     1501 gaaaccagcc aacgtgcggg gatatcctgg gtcgggcgcg gcaatgaaat actgtgcgtg
     1561 gggtgagagg cgaacattcc cggctggcga attgcctgat tcgcgccgca gtcaattagc
     1621 tccggcgggc gatcggcggt taagagaatc agtttttctc cggttaaccc ggcttcaatc
     1681 agtgccggat agagatttgc caccgccgtg ccggaggtca caatcaccgc caccggctgc
     1741 ttgctgactt tcgccagccc cagcgccaga tgccccagtc cacgctcatc gaaatgggtg
     1801 tggtgaatga atgcggaatt ctccgccgcc gctaacgtta acggtgtaga acgcgagcct
     1861 ggggcgatac agatgtgtct gacgccgtga cgcgttaatg cttccagaat gaccgccgcc
     1921 cagcgtcggt taaatgcgct tactgacatg agtttgtccg gtatcaatat tgcggctaag
     1981 tataaggagc tacaaaaata gattattgat atgaatcggt aatgatgcga ctcattacta
     2041 ttccatttgt aataaagtac gcagccctgc cgctttgttg tcgatttcct gccactcttg
     2101 ctcggggtcg gaaccacgga caatgcccgc gccagcatat agccgcacga cattgccgct
     2161 gatttttgct gaacgcaggg aaacgcagaa ttcgctttgt tgtaatgaga gatagcccgc
     2221 agaaccggcg taccattctc gggtgaacgg ttcgtgacgg gcgataaact gtcgcgccag
     2281 atcgcgcggt aagccagcaa ctgctgccgt tggctgcaac tgatgtaaac agatcacatc
     2341 atccgctttg ttgagtgaag tccagataca gcggcgaaga tgctgcactt tacgcagacg
     2401 cagtacctgc ggcggtaaaa catccagcgt ctgggtatcg gcctgtaatc gttggcagat
     2461 atcttccacc accagcatgt tctcgcgctg gtttttatca tccgccatca gccactctcc
     2521 taactgctgc gcctgcttat catcaggatt atttgctact gttcccgcca gcgcttcagt
     2581 acgcagcgct ttgtcacgcc gccgccataa ccgttccggt gaagagccaa gaaaagcatt
     2641 ttcgccatca aaggccatgt aaaaatggta gcaattcaga ttcagtcgac gactggcagc
     2701 catcatcgcc gccgcgttga ccggacttgc gaaatgcagg tcagttgccc gagcgagcac
     2761 cactttgtcg agctcacctt cggcgatggt tttcgttgcc agttcgatta attgcgtcca
     2821 gcccgttttg tccggccagt gttgttctcg cgtggtggtt aaatgtaacc caggcaaggg
     2881 cttgatactc accagtgtgg cgataaattc ttttgcctga atcgcatcgt gctgaaggga
     2941 gctttcgctg aatagcgtca gccgcagcgt ggctttaccg ccacagcgtc gccattccag
     3001 gcggggtaaa agtaaattgc cctgcgacgg gtcaaaagca ttcagccccc aaatgcgtaa
     3061 gtcggcgtgt tccgggtgct ggcgaagaaa acgttgtgcc tggtccaacg acgtaaaacg
     3121 ggtaatcgcg cccaggacgg ctgcttcttc atcaccatta cgttgttgcc agtagaattg
     3181 cgggtacgtt tgctgactgg ccagccagct caaggcatca aaagcgtctt tgagagggaa
     3241 aggaatatcg ataacccgaa tgccgggtgt cgccggaatc tcttgcgaca aatggcgcag
     3301 tagattttcc agcgccgtag taagtgattg cacgcggacc tctccctgtt aaaagcctca
     3361 cattatacgg ggtactacaa aaaaaatgca gtaccccggt gtagggaggt tttaacggcg
     3421 tgccagcaac agtcctagta ccagcccaac ggccgcgccc acaccaattc cttgccaggg
     3481 tttttcgtgg acgtagtcat cagcacgata aactgcctgc ttcgcccgat agtaataact
     3541 gtctgaagcc tggctaaccc gttttttcac atcatccagc gctttttctg cacgcgcttt
     3601 cagctcaaca tatttctgat cggcgggatc gccagaggag cggagcacct cttccagtgt
     3661 ttcactaagc agcgtcaggt cgtcatcgat acgtgtatca ccaaactgat tagacattct
     3721 cgttctccat ttgcgtaaaa cctgtcacta actatagaca agggttgttg attacgcctg
     3781 aattacctcg cgtgccatcc caatgtgtgg gataccatct tcttcataga cctccgtcac
     3841 agggataaaa ccgaaactct ggtagaagtt ttgcagatgc gcctgcgccc cgaggtaaac
     3901 aggcttatca ggccagtgat gcgtacatgt ttcgagtgtt ttactcatca gctgctgccc
     3961 cactttttcg ccacgcaacg cttcactgac aatcacccga cctataacga ccggctcaag
     4021 atcatcatca cttttcagaa tcctcgcata tgccaccagt tcatcatttt tccaaccgag
     4081 aatatggcga ttatcccccg tcaaatcatc accatcgata tcctgataag ggcaattctg
     4141 ctcgacaaca aacaccgcgc aacgcagttg taataaggca taaagctggg aaaccgataa
     4201 ttcagagtga tgcagatctt gccattcaat cataatgctc tccttttaga tgtatgcctt
     4261 atactagccg gaatattttt tgaaacgtga tgaactcatg gaattaattt ttttaggtac
     4321 ttcggccggt gtgccaaccc gcacgcgcaa tgtcacggca atattgctta acctgcaaca
     4381 tccgacccag agcggtcttt ggttgtttga ctgcggtgaa ggcacccagc atcagctact
     4441 gcataccgcc tttaaccctg gaaaactgga caagattttt atcagtcacc ttcatggcga
     4501 tcatcttttt ggtttacccg gcttgctgtg cagtcgttct atgtcaggca ttatccaacc
     4561 cttaacgatt tatggtccgc aaggtatccg tgaatttgtg gaaaccgcgc tgcggattag
     4621 cggctcatgg accgattatc cgctggaaat tgtcgaaatt ggcgctggcg aaattctcga
     4681 tgatggcctg cgcaaagtaa ccgcttatcc gctggaacac ccactggaat gttatggcta
     4741 tcgtattgaa gaacatgata aaccgggtgc attaaatgcc caggcattaa aagctgctgg
     4801 cgtgccgcct ggcccactgt ttcaggaatt aaaagcgggc aaaacaatca cgctggaaga
     4861 tggaaggcag attaacggcg cagattactt agctgctcca gtgccaggta aagcgctcgc
     4921 tattttcggc gataccggcc cctgcgatgc cgcacttgac ctggctaaag gtgtcgatgt
     4981 catggtgcac gaagcgacgc tggatataac catggaagcc aaagccaata gtcgcggcca
     5041 tagctctaca cgccaggctg cgacactagc ccgtgaggct ggagtcggta agctaatcat
     5101 tacccacgtc agctcgcgct atgatgacaa aggttgtcag cacctgttac gtgaatgcag
     5161 gtcaattttc ccggcgactg aactagcgaa tgatttcacc gtgtttaacg tttaactctg
     5221 cttattatta aaaacagggc gaaacttgcc ctgttatcgc aacccgcgct acatatatcc
     5281 acccacacgg gcatattgag cgatttttga tatttattca tctccttacg cactatattc
     5341 agagcaataa gttgcctctt tttatcttca ttcagcacaa cctttgcaac taaaaggagc
     5401 ccatgatggt tacagttgtc agcaattatt gtcaattatc tcaaacccaa ctcagtcaga
     5461 catttgcaga aaaatttact gtgaccgagg aattactgca gtctttaaaa aaaacagcgt
     5521 tatccggaga tgaagaaagt attgagttac tgcataatat tgcgttaggt tatgatgaat
     5581 ttggaaaaaa agctgaagat attctttacc atattgttag aaac
//
LOCUS       DAZKHF020000273         5426 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2201S31241
            SAMN43267800-rid25106413.denovo.275, whole genome shotgun sequence.
ACCESSION   DAZKHF020000273 DAZKHF020000000
VERSION     DAZKHF020000273.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267800
            Sequence Read Archive: SRR30307263
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5426)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5426)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 5426)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350581.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350581.2
            Genome Coverage       :: 151x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:21:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,652
            CDSs (total)                      :: 5,557
            Genes (coding)                    :: 5,320
            CDSs (with protein)               :: 5,320
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 237
            CDSs (without protein)            :: 237
            Pseudo Genes (ambiguous residues) :: 0 of 237
            Pseudo Genes (frameshifted)       :: 102 of 237
            Pseudo Genes (incomplete)         :: 130 of 237
            Pseudo Genes (internal stop)      :: 52 of 237
            Pseudo Genes (multiple problems)  :: 41 of 237
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5426
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267800-rid25106413.denovo.275"
                     /serotype="O 26:H11"
                     /isolate="2201S31241"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-01"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            <1..177
                     /locus_tag="ACHOSC_RS20840"
                     /old_locus_tag="pgaptmp_004168"
     CDS             <1..177
                     /locus_tag="ACHOSC_RS20840"
                     /old_locus_tag="pgaptmp_004168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415949.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HGU4549987.1"
                     /translation="RLSQSKFRCQVCGYTANADVNGARNILAAGHAVLACGEMVQSGR
                     SLKQEPTEMIQATA"
     gene            221..328
                     /locus_tag="ACHOSC_RS20845"
                     /old_locus_tag="pgaptmp_004169"
                     /pseudo
     CDS             221..328
                     /locus_tag="ACHOSC_RS20845"
                     /old_locus_tag="pgaptmp_004169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709992.3"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
     gene            complement(312..950)
                     /gene="ytfB"
                     /locus_tag="ACHOSC_RS20850"
                     /old_locus_tag="pgaptmp_004170"
     CDS             complement(312..950)
                     /gene="ytfB"
                     /locus_tag="ACHOSC_RS20850"
                     /old_locus_tag="pgaptmp_004170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418627.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein YtfB"
                     /protein_id="HGU4549988.1"
                     /translation="MPGRFELKPTLEKVWHAPDNFRFMDPLPPMHRRGIIIAAIVLVV
                     GFLLPSDDTPNAPVVTREAQLDIQSQSQPPTEEQLRAQLVTPQNDPDQVAPVAPEPIQ
                     EGQPEEQPQTTQTQPFQPDSGIDNQWRSYRVEPGKTMAQLFRDHGLPATDVYAMAQVE
                     GAGKPLSNLQNGQMVKIRQNASGVVTGLTIDTGNNQQVLFTRQPDGSFIRAR"
     gene            1169..1789
                     /gene="fklB"
                     /locus_tag="ACHOSC_RS20855"
                     /old_locus_tag="pgaptmp_004171"
     CDS             1169..1789
                     /gene="fklB"
                     /locus_tag="ACHOSC_RS20855"
                     /old_locus_tag="pgaptmp_004171"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015742582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FKBP-type peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HGU4549989.1"
                     /translation="MTTPTFDTIEAQASYGIGLQVGQQLSESGLEGLLPEALVAGIAD
                     ALEGKHPAVPVDVVHRALREIHERADAVRRQRFQAMAAEGVKYLEENAKKEGVNSTES
                     GLQFRVINQGEGAIPARTDRVRVHYTGKLIDGTVFDSSVARGEPAEFPVNGVIPGWIE
                     ALTLMPVGSKWELTIPQELAYGERGAGASIPPFSTLVFEVELLEIL"
     gene            2098..3501
                     /gene="cycA"
                     /locus_tag="ACHOSC_RS20860"
                     /old_locus_tag="pgaptmp_004172"
                     /gene_synonym="dagA"
     CDS             2098..3501
                     /gene="cycA"
                     /locus_tag="ACHOSC_RS20860"
                     /old_locus_tag="pgaptmp_004172"
                     /gene_synonym="dagA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001593878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-serine/D-alanine/glycine transporter"
                     /protein_id="HGU4549990.1"
                     /translation="MVDQVKVVADDQAPAEQSLRRNLTNRHIQLIAIGGAIGTGLFMG
                     SGKTISLAGPSIIFVYMIIGFMLFFVMRAMGELLLSNLEYKSFSDFASDLLGPWAGYF
                     TGWTYWFCWVVTGMADVVAITAYAQFWFPDLSDWVASLAVIVLLLTLNLATVKMFGEM
                     EFWFAMIKIVAIVSLIVVGLVMVAMHFQSPTGVEASFAHLWNDGGWFPKGLSGFFAGF
                     QIAVFAFVGIELVGTTAAETKDPEKSLPRAINSIPIRIIMFYVFALIVIMSVTPWSSV
                     VPEKSPFVELFVLVGLPAAASVINFVVLTSAASSANSGVFSTSRMLFGLAQEGVAPKA
                     FAKLSKRAVPAKGLTFSCICLLGGVVMLYVNPSVIGAFTMITTVSAILFMFVWTIILC
                     SYLVYRKQRPHLHEKSIYKMPLGKLMCWVCMAFFVFVIVLLTLEDDTRQALLVTPLWF
                     IALGLGWLFIGKKRMAK"
     gene            complement(3543..3641)
                     /locus_tag="ACHOSC_RS20865"
                     /old_locus_tag="pgaptmp_004173"
                     /pseudo
     CDS             complement(3543..3641)
                     /locus_tag="ACHOSC_RS20865"
                     /old_locus_tag="pgaptmp_004173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518748.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            3768..4196
                     /locus_tag="ACHOSC_RS20870"
                     /old_locus_tag="pgaptmp_004174"
                     /pseudo
     CDS             3768..4196
                     /locus_tag="ACHOSC_RS20870"
                     /old_locus_tag="pgaptmp_004174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016246814.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            4301..5368
                     /locus_tag="ACHOSC_RS20875"
                     /old_locus_tag="pgaptmp_004175"
     CDS             4301..5368
                     /locus_tag="ACHOSC_RS20875"
                     /old_locus_tag="pgaptmp_004175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001317586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4549991.1"
                     /translation="MAILHPQECWLLERIMSPEYYRRRFEGWQEFVELCERQVAEWSK
                     TMPLDVRRRPLCEQIDVVWGGRVLPNIRSTLKSVQYDFIQLQQGDLRVLQSGGNISSD
                     MKGLIDYPSDWMSLAAQKQYDRLKWRGAHYNNLIRRTSGGYWYDGELTYYYEESLHGP
                     LALPMQLPLYELDSSVYLREDDSVTVAGLYLPDIPDASAQLLYRSEHIPEAWQGRVRT
                     KYVNEAGIQEYYWESGAWAKCNWKRIRRVANRFINVPPEGFFPQGMPEELYNWPQREA
                     QYVTDRQRIAAFSGEACPHSGEWSIFVEGRQATVTLEQGEQMPEWTDRKMEGEYKRGE
                     KFHVLWSLMNRHDGGSVWVEA"
BASE COUNT         1226 a         1295 c         1512 g         1393 t
ORIGIN      
        1 cgcctgtcac aaagtaaatt cagatgccag gtatgtggat atacagcgaa cgccgatgta
       61 aacggcgctc gtaacatttt agcggcgggg cacgccgttc ttgcctgtgg agagatggtg
      121 cagtcaggcc gctcgttgaa gcaggaaccc accgaaatga ttcaggcgac agcctgaacg
      181 aagcagggat ccacgtcctt cagggcgtgg aggatgtcaa catatcagta tggtttcccc
      241 ccaggcactg gaagccagac gtaaggtcat caaagagaca atccttagct ggcttttggt
      301 tgacccttca tctaccgcgc acgaataaaa ctgccatccg gctggcgggt aaacaggacc
      361 tgttgattat tccccgtatc aatggttaag cccgtcacca cgccgctggc gttctggcgg
      421 attttcacca tttgcccgtt ttgcaaatta ctcagcggct tacccgcgcc ttctacctgc
      481 gccattgcat agacatcggt agcgggcagt ccgtggtcgc ggaacagctg cgccatcgtt
      541 ttaccgggtt cgacacggta agagcgccat tgattatcaa ttccgctatc tggctggaat
      601 ggttgcgttt gggtagtttg cggctgctct tctggctggc cttcctgtat aggttctgga
      661 gcgaccggag ccacctgatc aggatcattt tgtggggtga ctaattgcgc ccgcagctgt
      721 tcttctgtcg gcggttgtga ttgcgactga atatctaact gcgcttcacg cgtcaccacc
      781 ggcgcgttgg gcgtatcatc agatggaagc agaaatccga ccaccaatac aatggcggca
      841 ataatgatcc cccgacgatg catcggcggc agcgggtcca tgaagcgaaa attgtccggc
      901 gcgtgccaga ctttctccag ggttggtttt agttcaaagc gcccgggcat ggtttcctcc
      961 tgctccgcgt cttgttcctc aatcatagcc tatgaataag ctaacgctat gatgtccgtg
     1021 gtaaacccgc ctttattata ttcatacggg atattgctat tgtttctttt tccccgggat
     1081 ttgtcatcat tcctgcgaca aagttttacc caaagaagtg tggctgatat gctgcccgct
     1141 acttttatac cctaagaaag gaaatacgat gaccacccca acttttgaca ccatcgaagc
     1201 gcaagcaagc tacggcattg gtttgcaggt agggcaacaa ctgagtgaat ctggcctgga
     1261 agggctgctg ccagaagcac tggttgcagg tattgccgat gcgctggaag gcaaacatcc
     1321 ggctgttccg gttgatgtgg tgcatcgcgc gctgcgtgaa atccacgagc gcgccgatgc
     1381 cgttcgtcgt cagcgtttcc aggcgatggc tgctgaaggt gtgaaatacc tggaagaaaa
     1441 cgccaaaaaa gaaggtgtga atagcaccga atctggcctg caattccgcg tgatcaacca
     1501 gggtgaaggc gcaattccgg cacgtaccga ccgcgttcgt gttcattaca ccggtaaact
     1561 gatcgacggc accgtgtttg acagctccgt tgctcgtggt gaacccgctg aatttccggt
     1621 taatggcgtg atccctggct ggattgaagc actgactctg atgccggtag gttctaaatg
     1681 ggaactgact atcccgcagg aactggcata tggcgagcgc ggcgcaggcg catccatccc
     1741 tccgttcagc accctggtgt ttgaagtcga actgctggaa atcctctaag cagcgcattc
     1801 tgttcccctc gaaccagagg ggagcaggca ttcagcaata aacccttcag tttgccaaac
     1861 ggcgctattt tgtgttgcaa agaccccgta agcgtgtatt tttgtgagct gtttcgcgtt
     1921 atcaccgtga tatgacactc actttaaaca taaaattaac attagatcta aatcttagta
     1981 ttcatcccgc gtattgttac ctaatatcga tgagtcccga tacagattcg tcgtatcata
     2041 ggctgactaa aggccgtaga gcctgaacaa cacagacagg tacaggaaga aaaaaacatg
     2101 gtagatcagg taaaagtcgt tgccgatgat caggctccgg ctgaacagtc gctacggcgc
     2161 aatctcacaa accgacatat tcagcttatt gccattggcg gtgccattgg tacggggttg
     2221 tttatggggt ctggcaaaac gattagcctt gccgggccgt cgatcatttt cgtttatatg
     2281 atcatcggtt ttatgctctt tttcgtgatg cgggcaatgg gggaattgct gctttcgaat
     2341 ctggaataca aatcttttag tgacttcgct tccgatttac tcgggccgtg ggcaggatat
     2401 ttcaccggct ggacttactg gttctgctgg gttgtaaccg gtatggcaga cgtggtggcg
     2461 atcacggctt atgctcagtt ctggttcccc gatctctccg actgggtcgc ctcgctggcg
     2521 gtgatagtgc tgctgctgac gctcaatctc gccaccgtga aaatgttcgg tgagatggag
     2581 ttctggtttg cgatgatcaa aatcgtcgcc attgtgtcgc tgattgtcgt cggcctggtt
     2641 atggtggcga tgcactttca gtcaccgact ggtgtggaag cgtcattcgc gcatttgtgg
     2701 aatgatggcg gctggttccc gaaaggttta agtggcttct ttgccggatt ccagatagcg
     2761 gttttcgctt tcgtagggat tgagctggta ggtacaaccg ctgcggaaac caaagatccg
     2821 gagaaatcac tgccgcgtgc aattaactct attccgatcc gtatcattat gttctacgtc
     2881 ttcgcgctga ttgtaattat gtccgtgacg ccgtggagtt cggtagtccc ggagaaaagc
     2941 ccgtttgttg aactgttcgt gttggtgggt ttgcctgcgg ctgccagcgt gatcaacttt
     3001 gtggtgctga cctctgcggc gtcttccgct aacagcggcg tcttctctac cagccgtatg
     3061 ctgtttggcc tggcccagga aggtgttgca ccgaaagcgt tcgctaaact ctctaagcgc
     3121 gcagtacccg cgaaagggct gaccttctcc tgcatctgtc tgctcggcgg cgtggtgatg
     3181 ctgtatgtga atcccagcgt gattggcgcg ttcacgatga tcacgacggt ttccgcgatt
     3241 ctgtttatgt tcgtctggac gattatcctt tgctcgtacc tggtgtatcg caaacagcgt
     3301 cctcatctgc atgagaagtc gatctacaag atgccgctcg gcaagctgat gtgctgggta
     3361 tgtatggcgt tctttgtgtt cgtgatcgtg ctgctgacac tggaagatga cactcgccag
     3421 gcgctgctgg ttaccccgct gtggtttatc gcgctggggt tgggctggct gtttattggt
     3481 aaaaaacgca tggcgaagta atgccgctaa ggcacctgcg ggtgtctttc aggctgcaaa
     3541 attgcctgat gcgctacgct tatcaggcct acagaacctc tgcaatatat tgagtttgcg
     3601 tgcttttgta ggccgggtaa ggcgtttacg ccgcatccgg catgaacaaa gcacactttg
     3661 tcagcaatct cctttgtctt tatacattcc taaaaatact tcccgtcatc gacagctctc
     3721 aggcgcgggc ttaccgtgga tctttgccac tcattgcaga gttcactatg gctaagcaca
     3781 gttggttatt aatcgccgca ttacctctct ccatttcacc ttcctggggc gcagattttt
     3841 attaccgcca gcaggagaaa ggcacggttt atgttgccga acagaaggga gaaaaggatg
     3901 agatcctctc cgaactgccg ggcgttaatt tttcccgcct ctggcgtatt gccaatttag
     3961 ccaataacca agaaacccgc ttactgtccg attttaatcc cgataagttc gattgcgatg
     4021 atcggaataa ttgccagcat acctggctca ccgatggacg ctctgttctt tggtctggca
     4081 aagtcctgaa aaatccgccc ggtgaaccta atgtcgatgc cgccagtttt caggcattcg
     4141 gtgctttcgc tgccgataaa cgcagcgtct attttgacgg tcagcgtacc gatgatagat
     4201 gaagtggata tgtatatagc tgtgtctcag ttgcaaacaa atatttatgt accgaaataa
     4261 aagtatcaat agacaattaa ttaataaatc aggagataaa gtggctatat tacatccaca
     4321 ggagtgctgg ttgctggaaa gaataatgtc gccggaatat tatcggcgtc gttttgaggg
     4381 ctggcaggag tttgtcgagc tgtgcgagcg acaggtggca gagtggagca agaccatgcc
     4441 gctggatgtg cgccgtcgtc cgctgtgcga gcagattgac gtcgtctggg gcgggcgagt
     4501 gttgcccaat atccgcagta ctttaaagtc ggtacagtat gactttattc agctacagca
     4561 gggggacctg cgggtactgc aatccggggg caatattagc agtgatatga aaggactaat
     4621 tgattatccg tcggactgga tgtcactcgc cgcgcaaaaa cagtatgaca gactgaaatg
     4681 gcgaggtgca cattataata atttaatcag acgtacctcg ggcggatact ggtatgacgg
     4741 cgaactgact tattattatg aggaaagtct gcacggcccg ctggccctgc cgatgcaatt
     4801 accgttgtat gagctggata gtagcgtata cctgcgtgag gatgactcgg taacggtggc
     4861 tggcctgtat ctgccggata tcccagatgc cagcgcccaa ctgttgtacc ggagtgaaca
     4921 tattcccgag gcttggcagg ggcgggtacg aacgaagtat gtcaacgagg ctgggataca
     4981 agaatattac tgggaaagcg gggcgtgggc aaaatgcaat tggaaacgta tacggcgggt
     5041 ggcaaaccga tttatcaatg tgccgccgga gggatttttc ccacagggca tgccggagga
     5101 gttgtacaac tggccacagc gcgaagcgca gtatgttaca gaccggcagc ggatagcggc
     5161 ctttagcggt gaagcttgtc ctcatagcgg ggagtggtcg atttttgtag aaggtagaca
     5221 agcgacggtg acactggagc agggagagca gatgccagaa tggacggaca ggaaaatgga
     5281 gggggagtat aaaagaggcg agaaattcca tgtgctgtgg tcgctgatga accgccatga
     5341 tggcgggtcg gtgtgggtgg aagcgtagcg gtagctggat gtacacgtaa aaaccaggga
     5401 atgggtgatt ttattctggc ttatac
//
LOCUS       DAZKHF020000274         5404 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2201S31241
            SAMN43267800-rid25106413.denovo.276, whole genome shotgun sequence.
ACCESSION   DAZKHF020000274 DAZKHF020000000
VERSION     DAZKHF020000274.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267800
            Sequence Read Archive: SRR30307263
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5404)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5404)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 5404)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350581.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350581.2
            Genome Coverage       :: 151x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:21:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,652
            CDSs (total)                      :: 5,557
            Genes (coding)                    :: 5,320
            CDSs (with protein)               :: 5,320
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 237
            CDSs (without protein)            :: 237
            Pseudo Genes (ambiguous residues) :: 0 of 237
            Pseudo Genes (frameshifted)       :: 102 of 237
            Pseudo Genes (incomplete)         :: 130 of 237
            Pseudo Genes (internal stop)      :: 52 of 237
            Pseudo Genes (multiple problems)  :: 41 of 237
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5404
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267800-rid25106413.denovo.276"
                     /serotype="O 26:H11"
                     /isolate="2201S31241"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-01"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            217..2649
                     /gene="hsdR"
                     /locus_tag="ACHOSC_RS20880"
                     /old_locus_tag="pgaptmp_004176"
     CDS             217..2649
                     /gene="hsdR"
                     /locus_tag="ACHOSC_RS20880"
                     /old_locus_tag="pgaptmp_004176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0015666 - restriction
                     endodeoxyribonuclease activity [Evidence IEA];
                     GO_process: GO:0009307 - DNA restriction-modification
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EcoAI/FtnUII family type I restriction enzme
                     subunit R"
                     /protein_id="HGU4549992.1"
                     /translation="MAELNLSNLTEADIITKCVMPAILNAGWDNTTQIRQEVKLRDGK
                     VIVRGKVAARRTVKSADIVLYHKPGIPLAVIEAKANKHEIGKGMQQGIEYARLLDVPF
                     VFATNGDGFIFRDATAAEGECLEKQITLDDFPSPAELWQKFCLWKGYTQAQLPVITQD
                     YYDDGSGKSPRYYQLQAINKTIEAVSNGQNRVLLVMATGTGKTYTAFQIIWRLWKSKN
                     KKRILFLADRNILVDQTKNNDFQPFGTAMTKVSGRTIDPAYEIHLALYQAITGPEEDQ
                     KAFKQVAPDFFDLIVIDECHRGSASEDSAWREILDYFSSATQIGLTATPKETHEVSST
                     DYFGDPVYVYSLKEGIEDGFLAPYKVVRVDIDVDLQGWRPTKGQTDLNGEVIDDRIYN
                     QKDFDRTMVIDERTELVARTITDYLKRTNPMDKTIVFCNDIDHAERMRRALVNLNPEQ
                     VKKNDKYVMKITGDDEIGKAQLDNFINPKKAYPVIATTSELMTTGVDAKTCKLVVLDQ
                     NIQSMTKFKQIIGRGTRIDERYGKLWFTILDFKKATELFADERFDGIPEKVMDTTPED
                     IADPESDFEEKLEEISEHDDEQVTGVDEPPAPPYQVTDTDDVGPLPEEDEKKIRKFHV
                     NGVAVGVIAQRVQYYDADGKLVTESFKDYTRKTLLKEYASLDDFTRKWQDADRKEAII
                     HELEQQGIIWEVLAEEVGKDLDPFDMLCHVVYGQPPLTRKERAENVRKRNYFTKYSEA
                     AQAVLDNLLDKYADAGVQEIESIQVLKLKPFDSMGTLPEIIKTGFGDRNGYNQALSEL
                     ENEIYQLPPRSA"
     gene            2716..4185
                     /locus_tag="ACHOSC_RS20885"
                     /old_locus_tag="pgaptmp_004177"
     CDS             2716..4185
                     /locus_tag="ACHOSC_RS20885"
                     /old_locus_tag="pgaptmp_004177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313334.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008170 - N-methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-6 DNA methylase"
                     /protein_id="HGU4549993.1"
                     /translation="MSISSVIKSLQDIMRKDAGVDGDAQRLGQLSWLLFLKIFDAQEE
                     VLELEQDNYQYPIPQRYLWRSWAANAQGITGDSLLEFVNDDLFPALKNLTAPIDKNPR
                     GYVVKQAFSDAYNYMKNGTLLRQVINKLNEIDFTSASERHLFGDIYEQILKDLQSAGN
                     AGEFYTPRAVTRFMVDRVDPKLGESIMDPACGTGGFLACAFDHVKNKYVKSVADHQTL
                     QQQIHGVEKKQLPHLLATTNMLLHGIEVPVQIRHDNTLNKPLSSWDEQLDVIVTNPPF
                     GGTEEDGIEKNFPAEMKTRETADLFLQLIVEVLAKNGRAAVVLPDGTLFGEGVKTKIK
                     KLLTEECNLHTIVRLPNGVFNPYTGIKTNLLFFTKGQPTKEIWFYEHPYPAGVKNYSK
                     TKPMKFEEFQAEIDWWGNEADGFASRIENEQAWKVSIDDVIARNFNLDIKNPHQAETI
                     SHDPDELLAQYAKQQAEIQTLRNQLRDILGAALSGKEVN"
     gene            4185..>5404
                     /locus_tag="ACHOSC_RS20890"
                     /old_locus_tag="pgaptmp_004178"
     CDS             4185..>5404
                     /locus_tag="ACHOSC_RS20890"
                     /old_locus_tag="pgaptmp_004178"
                     /EC_number="3.1.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000110079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006304 - DNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease subunit S"
                     /protein_id="HGU4549994.1"
                     /translation="MSVEKLIVDHMETWTSALQTRSTAGRGSSGKIDLYGIKKLRELI
                     LELAVRGKLVPQDPNDEPASELLKRIAAEKAELVKQGKIKKQKPLPEISEEEKPFELP
                     EGWEWTRLINLGIWALGSGFPNVVQGSTDKEILMCKVSDMNLEGNEKFIFSTKNTISK
                     DLADEYKIKISEPGTIIFPKIGGAIATNKRRILVQDTAIDNNCLGIKPCDAISGEWFY
                     LILNTLDMSKYQSGTSIPAINQSVIGSIPIALPSLKMQEKIVSYVITLMSLCDQLEQQ
                     SLTSLDAHQQLVETLLGTLTDSQNAEELAENWARISEHFDTLFTTEASVDALKQTILQ
                     LAVMGKLVPQDPNDEPASELLKRIAQEKAQLVKEGKIKKQKPLPPISDEEKPFELPEG
                     WEWCKFGLTSEFING"
BASE COUNT         1609 a         1290 c         1298 g         1207 t
ORIGIN      
        1 aaattagcag aatgacatct aatgccttag tacaaaaata ttgtttcact ccttgcaaga
       61 accgaacatt ctccatatga atccttatcc tactattgat gaaactcaag aataccttta
      121 aaacagtgac ttaacgctga taatactctt tgaacagatg aattcatcta tttttctgtg
      181 tagaatttgc gcatcgcatt tagatgagaa caccccatgg cggaactgaa cctaagtaac
      241 ctgacggaag cagacatcat taccaaatgc gttatgccag ccattctcaa tgcaggctgg
      301 gacaacacaa cgcaaatcag acaggaggtc aaactccggg acggtaaagt catcgtacgt
      361 ggtaaagttg cggcacgcag aacggtaaaa tctgctgaca tcgtgctgta tcacaaacca
      421 ggcattccct tagccgtgat tgaagcaaaa gccaacaaac atgaaattgg caaagggatg
      481 caacagggca ttgaatatgc gcgcctgctg gacgttcctt ttgtttttgc caccaacggg
      541 gatggcttta tcttccgcga tgccaccgca gccgaaggtg aatgccttga aaagcaaata
      601 acgctggatg acttcccctc ccccgctgaa ctctggcaaa aattctgcct ctggaaagga
      661 tacacacaag ctcagcttcc ggtgattact caggactatt acgacgatgg cagcggtaaa
      721 tcgccacgtt attaccagct tcaggcaatc aacaaaacca ttgaagccgt ctccaacggg
      781 caaaaccgcg ttctgctggt catggcgacc ggaacaggga aaacctatac cgcattccag
      841 atcatctggc gcctgtggaa atcaaaaaat aaaaaacgca ttttgttcct tgccgatcgc
      901 aacattctgg tcgaccaaac caaaaataat gatttccagc catttggtac ggcaatgacc
      961 aaagtcagcg gacgcaccat tgatcccgct tatgaaattc acctcgcgct ctatcaggct
     1021 ataactggcc cggaggaaga ccaaaaagcg tttaaacaag tcgcaccaga tttcttcgat
     1081 ctgatcgtga tcgacgaatg ccatcgcggc agcgcatccg aagacagcgc ctggcgagag
     1141 atccttgatt acttcagttc cgccacccaa attggcttaa ccgccacgcc aaaagagacg
     1201 catgaagtct ccagcacgga ttacttcggc gatccggttt acgtctactc gctaaaagaa
     1261 gggatcgaag acggcttcct cgccccttat aaagttgtcc gtgttgatat tgatgttgat
     1321 ctgcaaggct ggcgcccaac caaagggcaa actgacttaa acggcgaagt gatcgacgat
     1381 cgtatctata accagaaaga tttcgatcgc acgatggtaa tcgacgaacg gacagaactg
     1441 gttgccagaa ccattaccga ctacctcaag cgcaccaacc cgatggataa aaccatcgtc
     1501 ttctgtaacg acatcgatca tgcagaacgt atgcgccgcg ccctggtgaa tctcaacccg
     1561 gagcaggtga aaaagaacga caaatacgtc atgaaaatca ccggcgatga tgaaattggc
     1621 aaagctcagt tggataactt catcaacccg aaaaaagcgt acccggttat cgcgaccact
     1681 tcagagctga tgaccactgg tgtggatgct aaaacctgca aactggtagt actggaccag
     1741 aacatccagt cgatgaccaa attcaagcaa attatcggtc gtggtacacg catcgacgaa
     1801 cgttacggca aactctggtt taccatcctc gactttaaaa aagccaccga actgtttgcc
     1861 gatgagcgtt tcgatggcat tcccgaaaaa gtcatggata ccacaccaga ggatatcgcc
     1921 gatccagaat ctgattttga agagaaactc gaagaaatca gcgaacatga cgacgaacag
     1981 gtaacaggcg ttgatgaacc gcctgcgcca ccataccagg ttaccgatac cgatgatgtc
     2041 ggcccacttc cggaagaaga cgagaagaaa atccgcaagt ttcacgtcaa cggtgtagcg
     2101 gtgggcgtta ttgcgcagcg tgttcagtat tacgatgccg acggtaaact ggttaccgaa
     2161 tcttttaaag attacacccg caaaacactg ctcaaagaat atgcctcgct ggatgacttt
     2221 acccgcaagt ggcaggacgc cgatcgcaaa gaagcgatca ttcacgagct ggagcaacag
     2281 gggatcatct gggaagtact ggcagaagaa gtcggtaaag atctcgaccc gttcgacatg
     2341 ctttgccacg tagtgtatgg tcagccgccg ttaacccgca aagagcgcgc cgagaacgtg
     2401 cgcaagcgga actacttcac aaaatactct gaagcagcgc aagccgtgct cgataatctg
     2461 ctggataaat acgccgatgc gggcgtacag gagatcgaaa gtattcaggt gctgaaactt
     2521 aaaccattcg acagcatggg cactttaccg gagattatta aaaccggatt tggcgaccgt
     2581 aacgggtata atcaggcgct cagcgagctg gaaaacgaaa tctaccaatt accgccccgc
     2641 tctgcttaac aactctcttt tgtgccttct gcaaccaaca tgttgcagaa ggccttgttt
     2701 ttcatggaat acaacatgtc tatcagttca gtaatcaaat cattacaaga tattatgcgc
     2761 aaagatgccg gtgtggatgg cgatgcgcag cgtctcggtc agctctcctg gctgctgttt
     2821 ttgaaaatct tcgatgccca ggaagaggtg ctggaactgg agcaggataa ctatcaatat
     2881 ccgatcccac agcgttattt atggcgcagt tgggccgcaa acgctcaggg cattaccggt
     2941 gattccctgc tggaattcgt taatgatgat ctgttcccgg cgttgaaaaa cctcactgcg
     3001 cctatcgata aaaacccacg cggctacgtg gtgaagcagg cgttcagcga tgcctataac
     3061 tatatgaaaa acggtacgct actgcgccag gtgatcaaca agctgaacga aattgacttt
     3121 accagcgcca gcgaacgcca tctgtttggt gatatttacg aacagatcct taaagatctg
     3181 caatctgcgg gcaatgctgg cgaattctat acgccacgcg ccgtcactcg ctttatggtg
     3241 gatcgcgttg atccgaaact cggcgaatcc attatggacc cggcctgcgg tacgggcggt
     3301 tttcttgcct gcgcatttga tcatgtaaag aacaaatacg tgaagagcgt cgccgatcat
     3361 cagacgctgc aacaacagat ccacggtgtt gagaaaaaac agcttccgca cctgctggcg
     3421 accaccaata tgctgctgca cggcattgaa gtaccggtac aaattcgtca cgacaacacc
     3481 ctgaacaaac cgctttcctc ctgggatgaa caactggatg tcattgttac caacccgccg
     3541 tttggtggca cggaagaaga cggtattgag aagaactttc cggcagagat gaaaacccgc
     3601 gaaacggcgg atctgttcct gcaactgatt gtggaagtac tggcgaaaaa cggtcgcgcg
     3661 gcggtggtat tgccggatgg cacactattt ggcgaaggcg ttaaaaccaa aatcaaaaaa
     3721 ctgcttaccg aagagtgcaa cctgcatacc atcgtgcgtt taccgaatgg ggtgtttaac
     3781 ccctataccg gcattaaaac caacctgttg ttctttacca aaggtcagcc aaccaaagag
     3841 atttggttct atgagcatcc gtatccggcg ggcgtaaaaa actacagcaa aaccaagccg
     3901 atgaagtttg aagagtttca ggcggagatc gactggtggg gtaacgaggc cgatggtttt
     3961 gccagccgca tcgagaatga gcaagcgtgg aaagtcagca ttgatgacgt gattgcgcgt
     4021 aacttcaatc tggatattaa aaacccacat caggcggaaa ccattagcca tgatccggac
     4081 gaactgttag cgcagtatgc aaaacagcag gcggagatcc agacgctgcg taatcaactg
     4141 cgcgatattc ttggcgctgc gctgtctggc aaggaggtta actaatgagt gtggaaaagc
     4201 tgatcgttga tcatatggaa acctggacct cggcgttgca aacccgttcc accgccgggc
     4261 gcggcagctc cggtaagatt gatttgtatg gtattaagaa attgcgtgag ctgattctgg
     4321 aactggcggt gcgaggtaaa ctggtgccgc aggatccgaa cgatgaaccg gcgtcggagc
     4381 tgctgaagcg tattgcggcg gaaaaagcag agctggtgaa gcaaggtaaa attaaaaagc
     4441 aaaaaccgct gccggaaatt agcgaggaag agaagccgtt tgaattgccg gaggggtggg
     4501 aatggactcg attaattaat cttggtatat gggctttagg gtctggtttt ccaaatgtgg
     4561 tacagggaag cactgataaa gaaattttga tgtgcaaagt tagcgacatg aatcttgaag
     4621 gtaacgagaa attcattttt tcgacaaaaa atacaatttc aaaagaccta gctgatgaat
     4681 ataaaattaa aatctcagaa cccggcacca tcattttccc taaaattggc ggagcaattg
     4741 caactaataa acgaagaatt ttagttcagg atacagctat tgataacaac tgtcttggaa
     4801 taaaaccatg tgatgcaata tcgggggagt ggttctatct aatccttaat actctcgata
     4861 tgtcgaagta tcaatcagga acctcaatac ccgcgataaa tcaaagtgtc ataggtagta
     4921 taccaattgc gcttccttct ttaaaaatgc aagagaagat tgtttcttat gtcataaccc
     4981 taatgtccct ttgcgaccaa ctggaacagc aatccctgac cagtctggac gcacatcagc
     5041 aactggttga aaccctgttg ggaacactta cagacagcca aaacgccgag gaactggctg
     5101 aaaactgggc gcgtattagc gagcatttcg acaccctatt taccaccgaa gccagcgtgg
     5161 atgcgttaaa acagaccatt ctgcaactgg ccgtaatggg caaacttgtg ccgcaggatc
     5221 cgaatgacga accagcctct gaactgctca aacgaattgc gcaggaaaaa gctcaactgg
     5281 tgaaagaagg aaaaataaaa aaacaaaaac cgttgccgcc aattagcgat gaggaaaaac
     5341 cgtttgaact tccggaaggg tgggagtggt gtaaatttgg tttaacatca gagtttataa
     5401 atgg
//
LOCUS       DAZKHF020000275         5281 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2201S31241
            SAMN43267800-rid25106413.denovo.277, whole genome shotgun sequence.
ACCESSION   DAZKHF020000275 DAZKHF020000000
VERSION     DAZKHF020000275.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267800
            Sequence Read Archive: SRR30307263
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5281)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5281)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 5281)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350581.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350581.2
            Genome Coverage       :: 151x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:21:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,652
            CDSs (total)                      :: 5,557
            Genes (coding)                    :: 5,320
            CDSs (with protein)               :: 5,320
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 237
            CDSs (without protein)            :: 237
            Pseudo Genes (ambiguous residues) :: 0 of 237
            Pseudo Genes (frameshifted)       :: 102 of 237
            Pseudo Genes (incomplete)         :: 130 of 237
            Pseudo Genes (internal stop)      :: 52 of 237
            Pseudo Genes (multiple problems)  :: 41 of 237
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5281
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267800-rid25106413.denovo.277"
                     /serotype="O 26:H11"
                     /isolate="2201S31241"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-01"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            complement(122..757)
                     /gene="msrQ"
                     /locus_tag="ACHOSC_RS20895"
                     /old_locus_tag="pgaptmp_004179"
     CDS             complement(122..757)
                     /gene="msrQ"
                     /locus_tag="ACHOSC_RS20895"
                     /old_locus_tag="pgaptmp_004179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-methionine-sulfoxide reductase
                     heme-binding subunit MsrQ"
                     /protein_id="HGU4549995.1"
                     /translation="MRLTAKQVTWLKVCLHLAGLLPFLWLVWAINHGGLGADPVKDIQ
                     HFTGRTALKFLLATLLITPLARYAKQPLLIRTRRLLGLWCFAWATLHLTSYALLELGV
                     NNLALLGKELITRPYLTLGIISWVILLALAFTSTQAMQRKLGKHWQQLHNFVYLVAIL
                     APIHYLWSVKIISPQPLIYAGLAVLLLALRYKKLLSLFNRLRKQVHNKLSV"
     gene            complement(758..1762)
                     /gene="msrP"
                     /locus_tag="ACHOSC_RS20900"
                     /old_locus_tag="pgaptmp_004180"
     CDS             complement(758..1762)
                     /gene="msrP"
                     /locus_tag="ACHOSC_RS20900"
                     /old_locus_tag="pgaptmp_004180"
                     /EC_number="1.8.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016667 - oxidoreductase activity, acting
                     on a sulfur group of donors [Evidence IEA];
                     GO_function: GO:0043546 - molybdopterin cofactor binding
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein-methionine-sulfoxide reductase catalytic
                     subunit MsrP"
                     /protein_id="HGU4549996.1"
                     /translation="MKKNQFLKESDVTAESVFFMTRRQVLKALGISAAALSLPHAAHA
                     DLLSWFKGNDRPPAPAGKPLEFSKPAAWQNDLPLTPADKVSGYNNFYEFGLDKADPAA
                     NAGSLKTDPWTLKISGEVAKPLTLDHDDLTRRFPLEERIYRMRCVEAWSMVVPWIGFP
                     LHKLLALAEPTSNAKYVAFETIYAPEQMPGQQDRFIGGGLKYPYVEGLRLDEAMHPLT
                     LMTVGVYGKALPPQNGAPVRLIVPWKYGFKGIKSIVSIKLTRERPPTTWNLAAPDEYG
                     FYANVNPHVDHPRWSQATERFIGSGGILDVQRQPTLLFNGYAEQVASLYRGLDLRENF
                     "
     gene            complement(1871..2284)
                     /gene="hiuH"
                     /locus_tag="ACHOSC_RS20905"
                     /old_locus_tag="pgaptmp_004181"
     CDS             complement(1871..2284)
                     /gene="hiuH"
                     /locus_tag="ACHOSC_RS20905"
                     /old_locus_tag="pgaptmp_004181"
                     /EC_number="3.5.2.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyisourate hydrolase"
                     /protein_id="HGU4549997.1"
                     /translation="MLKRYLVLSVVTAAFSLPSLVYAAQQNILSVHILNQQTGKPAAD
                     VTVTLEKKADNGWLQLNTAKTDKDGRIKALWPEQTATTGDYRVVFKTGDYFKKQNLES
                     FFPEIPVEFHINKVNEHYHVPLLLSQYGYSTYRGS"
     gene            2417..3088
                     /gene="hprR"
                     /locus_tag="ACHOSC_RS20910"
                     /old_locus_tag="pgaptmp_004182"
     CDS             2417..3088
                     /gene="hprR"
                     /locus_tag="ACHOSC_RS20910"
                     /old_locus_tag="pgaptmp_004182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707854.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor HprR"
                     /protein_id="HGU4549998.1"
                     /translation="MKILLIEDNQRTQEWVTQGLSEAGYVIDAVSDGRDGLYLALKDD
                     YALIILDIMLPGMDGWQILQTLRTAKQTPVICLTARDSVDDRVRGLDSGANDYLVKPF
                     SFSELLARVRAQLRQHHALNSTLEISGLRMDSVSQSVSRDNISITLTRKEFQLLWLLA
                     SRAGEIIPRTVIASEIWGINFDSDTNTVDVAIRRLRAKVDDPFPEKLIATIRGMGYSF
                     VAVKK"
     gene            3088..4446
                     /gene="hprS"
                     /locus_tag="ACHOSC_RS20915"
                     /old_locus_tag="pgaptmp_004183"
     CDS             3088..4446
                     /gene="hprS"
                     /locus_tag="ACHOSC_RS20915"
                     /old_locus_tag="pgaptmp_004183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     HprS"
                     /protein_id="HGU4549999.1"
                     /translation="MKRLSITVRLTLLFILLLSVAGAGIVWTLYNGLASELKWRDDTT
                     LINRTAQIKQLLIDGVNPDTLPVYFNRMMDVSQDILIIHGDSINKIVNRTNVSDDMLN
                     NIPATETISAAGIYRSIINDTEIDALRINIDEVSPSLTVTVAKLASARHNMLEQYKIN
                     SIIICIVAIVLCSVLSPLLIRTGLREIKKLSGVTEALNYNDSREPVEVSALPRELKPL
                     GQALNKMHHALVKDFERLSQFADDLAHELRTPINALLGQNQVTLSQTRSIAEYQKTIA
                     GNIEELENISRLTENILFLARADKNNVLVKLDSLFLNKEVENLLDYLEYLSDEKEICF
                     KVECNQQIFADKILLQRMLSNLIVNAIRYSPEKSRIHITSFLDTNGYLNIDIASPGTK
                     INEPEKLFRRFWRGDNSRHSEGQGLGLSLVKAIAELHGGSATYHYFNKHNVFRITLPQ
                     RN"
     gene            complement(4554..>5281)
                     /gene="hchA"
                     /locus_tag="ACHOSC_RS20920"
                     /old_locus_tag="pgaptmp_004184"
     CDS             complement(4554..>5281)
                     /gene="hchA"
                     /locus_tag="ACHOSC_RS20920"
                     /old_locus_tag="pgaptmp_004184"
                     /EC_number="3.5.1.124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0036524 - protein deglycase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="glyoxalase III HchA"
                     /protein_id="HGU4550000.1"
                     /translation="KPYRGKHKILVIAADERYLPTDNGKLFSTGNHPIETLLPLYHLH
                     AAGFEFEVATISGLMTKFEYWAMPHKDEKVMPFFEQHKSLFRNPKKIADVVVSLNADS
                     EYAAIFVPGGHGALIGLPESQDVAAALQWAIKNDRFVISLCHGPAAFLALRHGDNPLN
                     GYSICAFPDAADKQTPEIGYMPGHLTWYFGEELKKMGMNIINDDITGRVHKDRKVLTG
                     DTPFAANALGKLAAQEMLAAYAG"
BASE COUNT         1453 a         1149 c         1250 g         1429 t
ORIGIN      
        1 tatgtcgatg atttgaggtt gatgacaaac gaaacataac ttgatgtgat ctgcgtaggc
       61 cggatagggc gctcgcgccg catccggcag ggataacgcg tactttgtcg gcaatatgaa
      121 gttatacaga taatttgttg tgaacctgct tgcgtaaccg gttaaaaagg gaaagcaact
      181 tcttataccg taaggctaaa agcagtaccg ccagcccagc gtagatgagt ggctgcggtg
      241 aaataatctt cactgaccac aaataatgta ttggggccag gatcgcgaca agatagacga
      301 agttatgcaa ctgttgccag tgtttaccga gttttcgctg catcgcctgg gttgaagtga
      361 acgctaacgc aagcaaaatt acccagctga taatacctaa cgttaaataa ggccgggtaa
      421 ttaactcctt acctaataac gccagattat tcacgcccag ctccagcaat gcgtaactgg
      481 ttaaatgcag tgtcgcccag gcaaagcacc ataagcctaa caggcggcga gtgcgtatca
      541 ataacggctg ttttgcgtag cgtgccagag gtgtgattaa caaggtcgcc agcaaaaatt
      601 tcagtgcagt gcgaccagta aaatgctgaa tatctttcac cggatcggca cccaatccac
      661 cgtgattaat cgcccagacc agccagagaa acggcaacaa tccggcaaga tgcaggcaaa
      721 ctttcagcca tgtcacctgt tttgctgtca gacgcattta gaaattctcc cgcaaatcca
      781 ggccacgata cagcgatgcc acctgctcgg cgtaaccatt aaacagtagc gttggctggc
      841 gctgtacatc gaggatgccg cctgaaccaa taaatcgttc ggtagcctgt gaccagcgcg
      901 ggtgatcaac atgcggatta acgttggcgt aaaaaccgta ttcgtcaggc gctgccagat
      961 tccaggtggt tggcggacgc tcgcgggtca gcttaatact gactatcgat ttaatccctt
     1021 taaagccata tttccacggc acaatcagtc gcaccggcgc gccattttgt ggcggtaacg
     1081 ccttgccata aacacccacg gtcatcagtg tgagcggatg cattgcttcg tcgagacgca
     1141 atccttcgac ataaggatat ttcagcccgc caccgataaa gcggtcctgc tggcctggca
     1201 tttgttccgg tgcataaatt gtttcgaaag cgacatactt cgcattgctg gtgggttcgg
     1261 caagcgccag caatttgtgc agcggaaaac caatccacgg caccaccatc gaccatgctt
     1321 ccacgcagcg catacgataa atacgctctt ccagcggaaa gcgacgggtt aaatcatcgt
     1381 gatcgagggt caatggtttt gccacttcgc cgctgatttt cagtgtccat ggatcggttt
     1441 tcaggctacc agcattagcg gcgggatcgg ctttatccag cccgaattca tagaagttgt
     1501 tataaccgga gactttatct gctggcgtca gtggcaggtc attttgccag gcggcaggct
     1561 tgctgaactc caacggtttt ccggcggggg cgggtgggcg atcattccct ttgaaccagc
     1621 taagcagatc ggcatgcgca gcgtgaggca aagaaagtgc agccgcgctg atgcccagtg
     1681 cttttaacac ctgccgacgc gtcataaaga ataccgactc ggccgtaaca tctgattctt
     1741 ttaaaaattg attctttttc ataagccatc acactttttt gtctttagcc agcaagagcc
     1801 gtttactgtt aatctgtcag gaagtgtcct ccctaacagt agcagagatc tgcttttaca
     1861 tatttatgca ttaactgcca cgataggttg aatacccata ttggctaagt aataaaggca
     1921 catgataatg ctcgttcact ttattaatat gaaattcaac ggggatctca gggaagaaac
     1981 tttcaagatt ttgtttcttg aaatagtccc cggttttaaa tacgacacgg taatcgcccg
     2041 tagttgcagt ttgctcgggc cacagtgcct taattcgtcc atccttatct gttttggcgg
     2101 tattaagttg taaccagccg ttgtccgctt tcttttcaag agtgactgtc acgtcggcag
     2161 cgggttttcc tgtttgctgg ttcaaaatgt gcacactaag aatgttttgt tgtgcagcat
     2221 agaccaaaga aggtaatgaa aatgcagccg ttactacgga gagtactaaa taacgcttta
     2281 acatgtttat atccttgtca tgtgaatgag ttttgaggat agcgaaaaag cgtccacggt
     2341 aaccttacag cagcattaca attttgtaat gaaccaggct gtttctataa catatgattt
     2401 atggcatatt attttcatga agattttact tattgaagat aatcaaagga cccaggagtg
     2461 ggtaacgcag gggctttccg aagcgggtta tgtcattgat gccgtttctg atggcagaga
     2521 tgggctttat cttgcgctta aggatgatta tgcattgatc attctggata ttatgcttcc
     2581 gggtatggat ggctggcaga tcttacaaac gttaagaaca gcaaagcaaa ctcctgttat
     2641 ttgccttact gcaagagatt ctgtcgatga cagagtcaga gggctggaca gtggggcaaa
     2701 tgattatctg gtaaaacctt tttcattttc tgagttgctg gcaagggttc gggcacaatt
     2761 aaggcaacat cacgctttga attcaacatt agaaatcagt ggcttaagaa tggactctgt
     2821 tagtcaaagt gtgagcaggg acaatatcag tattacactg acgcgcaagg agtttcagtt
     2881 actttggcta ctggcctcca gagctggcga gattatcccc agaacggtta ttgcgagtga
     2941 aatttgggga atcaactttg atagtgatac caatacggtg gacgtcgcca ttcgcaggct
     3001 ccgcgcaaaa gttgatgatc cttttcctga aaagctaatc gccacaatcc gggggatggg
     3061 ctattcattc gtagcggtaa aaaaataatg aaaagattat ctataaccgt ccgtttaacc
     3121 ttgcttttta tattgctgct atctgttgct ggcgctggaa ttgtatggac tctctataat
     3181 ggcctggcaa gtgagttgaa atggcgcgat gatacaacac tcattaaccg gacagcgcag
     3241 atcaagcagt tgttaattga tggggtaaat ccagatacgt tacctgtgta ctttaaccgg
     3301 atgatggatg ttagtcagga tatcttgatc attcatggtg atagcatcaa caaaattgtt
     3361 aaccggacga atgtcagtga tgacatgtta aataacatac ctgctactga gacaatcagc
     3421 gcagctggca tttacagaag cattattaat gatacagaga tagatgcttt acgaattaat
     3481 attgatgaag tttcgccatc attaacggtt actgtggcta aattggcttc agccagacat
     3541 aacatgcttg aacagtataa aattaatagc attataattt gcattgtcgc cattgtactt
     3601 tgctcagtat taagtccgct gttaatcaga acgggattac gagagatcaa aaagttgagt
     3661 ggtgtaacgg aagcgctgaa ttataacgat agccgggagc ctgttgaggt tagcgcatta
     3721 ccgagagaac taaaacctct tgggcaggcg ttgaataaaa tgcatcatgc tttagtcaaa
     3781 gattttgagc gtctaagtca gtttgctgac gatctcgctc atgaacttag aacgcccatt
     3841 aatgcattac tgggtcagaa tcaggttacg ctcagtcaaa ccagaagtat cgctgaatat
     3901 caaaaaacaa ttgccggtaa cattgaagag ctggaaaata tttcgcggtt aacagagaac
     3961 atactgtttc ttgcccgggc agataaaaac aatgttttgg tgaaactgga ctcacttttt
     4021 ctcaataagg aagtcgaaaa tttgttggat tatcttgaat acctttcaga cgagaaagag
     4081 atttgcttta aggtcgagtg caatcagcaa atctttgcgg ataaaatttt actgcaacga
     4141 atgttatcga atcttattgt taatgccatt agatattcac cagaaaaatc gcgcattcat
     4201 ataaccagtt ttcttgatac caacggctat cttaatattg atatcgccag tcctggaacg
     4261 aaaattaatg agcctgaaaa actcttccgt agattttggc ggggagataa ttcgcgtcat
     4321 tccgaaggtc agggacttgg tctttcttta gtcaaagcga ttgccgaatt gcatggggga
     4381 agtgctacgt atcactattt taataagcat aatgtgttcc gaattacgtt accgcaaaga
     4441 aattaacata gggcttcagt acgccataaa tcaaccatga aaaaacgcag ccacgtcgtg
     4501 agtattaacg tggctgcgat tgattatgcg cttacattca tacgtaacag ggattaaccc
     4561 gcataagctg ccagcatttc ctgcgccgcc agtttaccca acgcattcgc tgcgaaagga
     4621 gtgtcgccgg tgagaacttt acggtcctta tgtactcgcc cggtgatgtc gtcattaata
     4681 atgttcatgc ccattttctt cagctcttcg ccgaagtacc aggtgagatg acccggcata
     4741 tagcctatct ctggcgtttg tttgtctgcg gcgtctggga atgcgcaaat ggaataacca
     4801 ttcagtgggt tatcgccgtg gcgaagcgcc agaaaagccg ccgggccgtg gcaaagggag
     4861 ataacaaaac ggtcattttt gattgcccac tgcaaagcag cagccacgtc ctggctttct
     4921 ggtaaaccaa taagtgcgcc atgaccacca ggaacaaaga ttgctgcata ttcgctgtct
     4981 gcgttgaggc tgacgacaac atccgcgatt tttttcggat tgcggaacaa cgatttatgc
     5041 tgctcaaaga atggcattac tttttcatct ttgtgcggca tagcccagta ttcaaacttg
     5101 gtcatcagac cggaaatggt cgccacttcg aattcgaaac ctgcagcatg gagatgatac
     5161 aacggcagca acgtttcaat cggatggtta ccggtcgaga acagttttcc gttatcggtc
     5221 ggcaaataac gttcgtccgc ggcgatcacc agaattttat gtttaccgcg atacggtttt
     5281 g
//
LOCUS       DAZKHF020000276         5261 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2201S31241
            SAMN43267800-rid25106413.denovo.278, whole genome shotgun sequence.
ACCESSION   DAZKHF020000276 DAZKHF020000000
VERSION     DAZKHF020000276.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267800
            Sequence Read Archive: SRR30307263
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5261)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5261)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 5261)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350581.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350581.2
            Genome Coverage       :: 151x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:21:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,652
            CDSs (total)                      :: 5,557
            Genes (coding)                    :: 5,320
            CDSs (with protein)               :: 5,320
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 237
            CDSs (without protein)            :: 237
            Pseudo Genes (ambiguous residues) :: 0 of 237
            Pseudo Genes (frameshifted)       :: 102 of 237
            Pseudo Genes (incomplete)         :: 130 of 237
            Pseudo Genes (internal stop)      :: 52 of 237
            Pseudo Genes (multiple problems)  :: 41 of 237
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5261
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267800-rid25106413.denovo.278"
                     /serotype="O 26:H11"
                     /isolate="2201S31241"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-01"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            252..2957
                     /gene="malT"
                     /locus_tag="ACHOSC_RS20925"
                     /old_locus_tag="pgaptmp_004185"
     CDS             252..2957
                     /gene="malT"
                     /locus_tag="ACHOSC_RS20925"
                     /old_locus_tag="pgaptmp_004185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator MalT"
                     /protein_id="HGU4550001.1"
                     /translation="MLIPSKLSRPVRLDHTVVRERLLAKLSGANNFRLALITSPAGYG
                     KTTLISQWAAGKNDIGWYSLDEGDNQQERFASYLIAAVQQATNGHCAICETMAQKRQY
                     ASLTSLFAQLFIELAEWHSPLYLVIDDYHLITNPVIHESMRFFIRHQPENLTLVVLSR
                     NLPQLGIANLRVRDQLLEIGSQQLAFTHQEAKQFFDCRLSSPIEAAESSRICDDVSGW
                     ATALQLIALSARQNTHSAHKSARRLAGINASHLSDYLVDEVLDNVDLATRHFLLKSAI
                     LRSMNDALITRVTGEENGQMRLEEIERQGLFLQRMDDTGEWFCYHPLFGNFLRQRCQW
                     ELAAELPEIHRAAAESWMAQGFPSEAIHHALAAGDALMLRDILLNHAWSLFNHSELSL
                     LEESLKALPWDSLLENPQLVLLQAWLMQSQHRYGEVNTLLARAEHEIKDIREGTMHAE
                     FNALRAQVAINDGNPDEAERLAKLALEELPPGWFYSRIVATSVLGEVLHCKGELTRSL
                     ALMQQTEQMARQHDVWHYALWSLIQQSEILFAQGFLQTAWETQEKAFQLINEQHLEQL
                     PMHEFLVRIRAQLLWAWARLDEAEASARSGIEVLSSYQPQQQLQCLAMLIQCSLARGD
                     LDNARSQLNRLENLLGNGKYHSDWISNANKVRVIYWQMTGDKAAAANWLRHTAKPEFA
                     NNHFLQGQWRNIARAQILLGEFEPAEIVLEELNENARSLRLMSDLNRNLLLLNQLYWQ
                     AGRKSDAQRVLLDALKLANRTGFISHFVIEGEAMAQQLRQLIQLNTLPELEQHRAQRI
                     LREINQHHRHKFAHFDENFVERLLNHPEVPELIRTSPLTQREWQVLGLIYSGYSNEQI
                     AGELEVAATTIKTHIRNLYQKLGVAHRQAAVQHAQKLLKMMGYGV"
     gene            complement(3000..4016)
                     /gene="rtcA"
                     /locus_tag="ACHOSC_RS20930"
                     /old_locus_tag="pgaptmp_004186"
     CDS             complement(3000..4016)
                     /gene="rtcA"
                     /locus_tag="ACHOSC_RS20930"
                     /old_locus_tag="pgaptmp_004186"
                     /EC_number="6.5.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003963 - RNA-3'-phosphate cyclase
                     activity [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA 3'-terminal phosphate cyclase"
                     /protein_id="HGU4550002.1"
                     /translation="MKRMIALDGAQGEGGGQILRSALSLSMITGQPFTITSIRAGRAK
                     PGLLRQHLTAVKAAAEICRATVEGAELGSQRLVFRPGTVRGGEYRFAIGSAGSCTLVL
                     QTVLPALWFADGPSRVEVSGGTDNPSAPPADFIRRVLEPLLAKIGIHQQTTLLRHGFY
                     PAGGGVVATEVSPVASFNTLQLGERGNIVQMRGEVLLAGVPRHVAEREIATLAGSFSL
                     HEQNIHNLPRDQGPGNTVSLEVESENITERFFVVGEKRVSAEVVAAQLVKEVKRYLAS
                     PAAVGEYLADQLVLPMALAGAGEFKVAHPSCHLLTNIAVVERFLPVRFGLIETDGVTR
                     VSIE"
     gene            complement(4020..5246)
                     /gene="rtcB"
                     /locus_tag="ACHOSC_RS20935"
                     /old_locus_tag="pgaptmp_004187"
     CDS             complement(4020..5246)
                     /gene="rtcB"
                     /locus_tag="ACHOSC_RS20935"
                     /old_locus_tag="pgaptmp_004187"
                     /EC_number="6.5.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-splicing ligase RtcB"
                     /protein_id="HGU4550003.1"
                     /translation="MNYELLTTENAPVKMWTKGVPVEADARQQLINTAKMPFIFKHIA
                     VMPDVHLGKGSTIGSVIPTKGAIIPAAVGVDIGCGMNALRTALTAADLPENLAELRQA
                     IETAVPHGRTTGRCKRDKGAWENPPVNVDAKWAELEAGYQWLTQKYPRFLNTNNYKHL
                     GTLGTGNHFIEICLDESDQVWIMLHSGSRGIGNAIGTYFIDLAQKEMQDQLETLPSRD
                     LAYFMEGTEYFDDYLRAVAWAQLFASLNRDAMMENVVTALQSITQKTVRQPQTLAMEE
                     INCHHNYVQKEQHFGEEIYVTRKGAVSARAGQYGIIPGSMGAKSFIVRGLGNEESFCS
                     CSHGAGRVMSRTKAKKLFSVEDQIRATAHVECRKDAEVIDEIPMAYKDIDAVMAAQSD
                     LVEVIYTLRQVVCVKG"
BASE COUNT         1241 a         1485 c         1325 g         1210 t
ORIGIN      
        1 tcaggaatga agaagaaacc cttgcttaaa tgaatctgat gaacataagg gaaaccagta
       61 ttcacgctgg atcagcgtcg ttttaggtga gttgttaata aagatttgga attgtgacac
      121 agtgcaaatt cagacacata aaaaaacgtc atcgcttgca ttagaaaggt ttctggccga
      181 ccttataacc attaattacg aagcgcaaaa aaaataatat ttcctcattt tccacagtga
      241 agtgattaac tatgctgatt ccgtcaaaac taagtcgtcc ggttcgactc gaccataccg
      301 tggttcgtga gcgcctgctg gctaaacttt ccggcgcgaa caacttccgg ctggcgctga
      361 tcacgagtcc tgcgggctac ggaaagacca ccctcatttc ccagtgggcg gcaggcaaaa
      421 acgatatcgg ctggtactcg ctggatgaag gtgataacca gcaagagcgt ttcgccagct
      481 atctcattgc cgccgtgcag caggcaacca acggtcactg tgcgatatgt gagacgatgg
      541 cgcaaaaacg gcaatatgcc agcctgacgt cactcttcgc ccagcttttc attgagctgg
      601 cggaatggca tagcccactt tatctggtca tcgatgacta tcatctgatc actaatccag
      661 tgatccacga gtcaatgcgc ttctttattc gccatcaacc agaaaatctc accctggtgg
      721 tgttgtcacg caaccttccg caactgggca ttgccaatct gcgtgttcgt gatcaactgc
      781 tggaaattgg cagtcagcaa ctggcattta cccatcagga agcgaagcag ttttttgatt
      841 gccgtctgtc atcgccgatt gaagctgcag aaagcagtcg gatttgcgat gacgtttccg
      901 gttgggcgac ggcactacag ctaatcgccc tctccgcccg gcagaatacc cactcagccc
      961 ataagtcggc acgccgcctg gcgggaatca atgccagcca tctttcggat tatctggtcg
     1021 atgaggtttt ggataacgtc gatctcgcaa cgcgccattt tctgttgaaa agcgccattt
     1081 tgcgctcaat gaacgatgcc ctcatcaccc gtgtgaccgg cgaagaaaac gggcaaatgc
     1141 gtctcgaaga gattgagcgt caggggctgt ttttacagcg gatggatgat accggcgagt
     1201 ggttctgcta tcacccgctg tttggcaact tcctgcgcca gcgctgccag tgggaactgg
     1261 cggcggagct gccggaaatc caccgtgccg ccgcagaaag ctggatggcc cagggatttc
     1321 ccagcgaagc gattcatcat gcgctggcgg caggcgatgc gctgatgctg cgcgatattc
     1381 tgcttaatca cgcctggagt ttgttcaacc atagcgaact gtcgctgctg gaagagtcgc
     1441 ttaaggccct gccgtgggac agtttgctgg aaaatccgca gttagtgtta ttgcaggcgt
     1501 ggctaatgca aagccaacat cgctatggcg aagttaacac cctgctagcc cgtgctgaac
     1561 atgaaatcaa ggacatcaga gaaggcacca tgcacgcaga atttaacgct ctgcgcgccc
     1621 aggtggcgat taacgatggt aatccggatg aagcggaacg gctggcaaaa ctggcactgg
     1681 aagagctgcc gccgggctgg ttctatagcc gcattgtggc aacctcggtg ctgggtgaag
     1741 tgctgcactg caaaggcgaa ttgacccgct cactggcgct aatgcagcaa accgaacaga
     1801 tggcacgcca gcacgacgtc tggcactacg ccttatggag tttaatccag caaagtgaaa
     1861 ttctgtttgc ccaagggttc ctgcaaaccg cgtgggaaac acaggaaaaa gcgttccagc
     1921 tgatcaacga gcagcatctg gaacagctgc cgatgcatga gtttctggtg cgcatccgag
     1981 cacaactgtt atgggcctgg gcacggctgg atgaagccga agcgtcggcg cgtagcggga
     2041 ttgaagtctt gtcgtcttat cagccacagc aacagcttca gtgcctggca atgttgattc
     2101 aatgctcgct ggcccgtggt gatttagata acgcccgtag ccagctgaac cgtctggaaa
     2161 acctgctggg gaatggcaaa tatcacagcg actggatctc taacgccaac aaagtccggg
     2221 tgatttactg gcaaatgacc ggcgataaag ccgccgctgc caactggttg cgtcatacgg
     2281 ctaaaccgga gtttgcgaac aaccacttcc tgcaaggtca atggcgcaac attgcccgcg
     2341 cacaaatctt gctgggcgag tttgaacctg cggaaattgt tctcgaagaa ctcaatgaaa
     2401 atgcccgcag tctgcggttg atgagcgatc tcaaccgtaa cctgttgctg cttaatcaac
     2461 tgtactggca ggccggacgt aaaagtgacg cccagcgcgt gttgctggac gcattaaaac
     2521 tggcgaatcg caccggattt atcagccatt ttgtcatcga aggcgaagcg atggcgcaac
     2581 aactgcgcca gctgattcaa ctcaatacgc tgccggaact ggaacagcat cgcgcacagc
     2641 gtattctgcg agaaatcaat caacatcatc ggcataaatt cgcccatttc gatgagaatt
     2701 tcgttgaacg tctgctaaat catccggaag tgccagaact gatccgcacc agcccgttga
     2761 cccaacgtga atggcaggtc ctggggctga tttactccgg ttacagcaat gaacaaattg
     2821 cgggcgaact ggaagtggcg gcaaccacca tcaagacgca tatccgcaat ctgtaccaaa
     2881 aactcggcgt cgcccaccgt caggctgccg tgcagcacgc gcagaaactg ctgaagatga
     2941 tgggttatgg ggtgtgagtt tagccggata atgcattgga tccggtttac atctttacat
     3001 cattcaatac tcacccgcgt tacgccatct gtttctatca aaccaaaccg caccggcaag
     3061 aaacgctcca ccaccgcgat attggtcagc agatggcatg aaggatgggc gaccttaaat
     3121 tctcccgcgc ccgccagcgc catcggcagt accagttggt cggcgagata ttcccccacc
     3181 gccgctgggc ttgccaggta gcgtttcacc tctttcacta actgcgcggc taccacctcg
     3241 gcactgacgc gcttttcacc gacgacgaaa aagcgttcag tgatattttc actttcgact
     3301 tccagcgaga cggtattacc cggcccctgg tcgcgcggca ggttatgaat attctgttca
     3361 tgcagggaaa aactccccgc cagtgtagcg atttcacgct cagcaacatg gcgcggcaca
     3421 ccagctaata gaacttctcc acgcatctgc acaatgttcc cgcgctcgcc aagttgcaag
     3481 gtgttaaacg atgccacggg cgagacttcc gttgccacca cgccgcctcc ggcaggataa
     3541 aaaccgtgac gtaacagcgt ggtttgctga tgaattccta ttttcgccag cagcggctcc
     3601 agcacccggc ggataaaatc cgcaggcggg gccgacgggt tatcggtgcc tccgctcact
     3661 tcaacacgcg aaggtccatc ggcaaaccac agcgcgggca gcaccgtttg cagcaccagc
     3721 gtacagcttc cggcactacc gatagcaaag cggtactcgc cgccgcgcac ggtgccaggc
     3781 cggaagacca gacgctgcga ccccagctcc gcgccttcca ccgttgccct acaaatttcc
     3841 gcagccgctt ttaccgcggt cagatgctgg cgcaacagcc ccggtttcgc ccgcccggca
     3901 cgaatgctgg tgatggtaaa tggctggccg gttatcatcg acaggctcag cgccgagcgc
     3961 aggatctgcc cgccaccttc gccctgtgcg ccatccagcg caatcatcct tttcatcatt
     4021 tatcctttta cgcacaccac ctgacgcagg gtatagataa cttccaccag atcgctttgt
     4081 gccgccatca ccgcatcaat atctttatac gccatcggga tttcgtcgat cacttcggca
     4141 tctttacggc attccacatg cgcggtggca cgaatttgat cttccacgct gaacagtttt
     4201 ttcgctttag tacggctcat tacccgcccg gcaccgtggc tgcacgaaca gaacgactct
     4261 tcatttccca gcccacggac gataaagctt tttgctccca tcgaaccggg aataattcca
     4321 tattgaccag cacgcgcaga caccgcgcct ttacgcgtca cgtagatctc ttcaccaaag
     4381 tgctgttctt tttgcacata gttgtggtga cagttgatct cttccatcgc cagcgtttgt
     4441 ggctgtctga ccgttttctg cgtaatgctc tgcaacgccg ttaccacgtt ttccatcatc
     4501 gcatcgcggt taaggctggc gaaaagctgc gcccaggcca cggctcttag gtaatcatca
     4561 aagtattccg taccttccat aaagtacgcc aggtcgcgcg atggcaacgt ttcaagctgg
     4621 tcctgcatct ctttttgtgc caaatcgata aagtaagtcc cgatggcgtt accaattccg
     4681 cgtgaaccgg agtgcagcat aatccacacc tggtccgact catcaaggca gatttcaata
     4741 aagtggttac ccgttcccag cgttcccagg tgtttatagt tgttagtgtt aaggaaacgc
     4801 ggatattttt gcgttaacca ctgataaccg gcttcaagct cagcccattt cgcatcgacg
     4861 ttaacaggcg ggttttccca tgcgccttta tcacgtttac aacggccagt ggtacgtccg
     4921 tgcggcaccg cagtttcaat cgcctgacgc agctctgcca ggttttcagg caggtctgcc
     4981 gccgttaacg cggtacgcag cgcgttcatt ccacagccaa tatccacgcc caccgccgcc
     5041 ggaataatcg cccctttggt cgggatcacg ctaccaatgg tggaaccttt acccaggtgt
     5101 acatcaggca ttaccgcaat atgtttgaaa ataaacggca tcttcgccgt attaataagt
     5161 tgctgacgcg catcggcttc taccggcacg cctttggtcc acatttttac cggggcattt
     5221 tcagtggtca gtaattcgta attcattttg tttttctctt t
//
LOCUS       DAZKHF020000277         5248 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2201S31241
            SAMN43267800-rid25106413.denovo.279, whole genome shotgun sequence.
ACCESSION   DAZKHF020000277 DAZKHF020000000
VERSION     DAZKHF020000277.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267800
            Sequence Read Archive: SRR30307263
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5248)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5248)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 5248)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350581.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350581.2
            Genome Coverage       :: 151x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:21:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,652
            CDSs (total)                      :: 5,557
            Genes (coding)                    :: 5,320
            CDSs (with protein)               :: 5,320
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 237
            CDSs (without protein)            :: 237
            Pseudo Genes (ambiguous residues) :: 0 of 237
            Pseudo Genes (frameshifted)       :: 102 of 237
            Pseudo Genes (incomplete)         :: 130 of 237
            Pseudo Genes (internal stop)      :: 52 of 237
            Pseudo Genes (multiple problems)  :: 41 of 237
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5248
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267800-rid25106413.denovo.279"
                     /serotype="O 26:H11"
                     /isolate="2201S31241"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-01"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            95..1429
                     /gene="cadB"
                     /locus_tag="ACHOSC_RS20940"
                     /old_locus_tag="pgaptmp_004188"
     CDS             95..1429
                     /gene="cadB"
                     /locus_tag="ACHOSC_RS20940"
                     /old_locus_tag="pgaptmp_004188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043872 - lysine:cadaverine antiporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cadaverine/lysine antiporter"
                     /protein_id="HGU4550004.1"
                     /translation="MSSAKKIGLFACTGVVAGNMMGSGIALLPANLASIGGIAIWGWI
                     ISIIGAMSLAYVYARLATKNPQQGGPIAYAGEISPAFGFQTGVLYYHANWIGNLAIGI
                     TAVSYLSTFFPVLNDPVPAGIACIAIVWVFTFVNMLGGTWVSRLTTIGLVLVLIPVVM
                     TAIVGWHWFDAATYAANWNTADTTDGHAIIKSILLCLWAFVGVESAAVSTGMVKNPKR
                     TVPLATMLGTGLAGIVYIAATQVLSGMYPSSVMAASGAPFAISASTILGNWAAPLVSA
                     FTAFACLTSLGSWMMLVGQAGVRAANDGNFPKVYGEVDSNGIPKKGLLLAAVKMTALM
                     ILITLMNSAGGKASDLFGELTGIAVLLTMLPYFYSCVDLIRFEGVNIRNFVSLICSVL
                     GCVFCFIALMGASSFELAGTFIVSLIILMFYARKMHERQSHSMDNHTASNAH"
     gene            1509..3656
                     /gene="cadA"
                     /locus_tag="ACHOSC_RS20945"
                     /old_locus_tag="pgaptmp_004189"
     CDS             1509..3656
                     /gene="cadA"
                     /locus_tag="ACHOSC_RS20945"
                     /old_locus_tag="pgaptmp_004189"
                     /EC_number="4.1.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine decarboxylase CadA"
                     /protein_id="HGU4550005.1"
                     /translation="MNVIAILNHMGVYFKEEPIRELHRALERLNFQIVYPNDRDDLLK
                     LIENNARLCGVIFDWDKYNLELCEEISKMNENLPLYAFANTYSTLDVSLNDLRLQISF
                     FEYALGAAEDIANKIKQTTDEYINTILPPLTKALFKYVREGKYTFCTPGHMGGTAFQK
                     SPVGSLFYDFFGPNTMKSDISISVSELGSLLDHSGPHKEAEQYIARVFNADRSYMVTN
                     GTSTANKIVGMYSAPAGSTILIDRNCHKSLTHLMMMSDVTPIYFRPTRNAYGILGGIP
                     QSEFQHATIAKRVKETPNATWPVHAVITNSTYDGLLYNTDFIKKTLDVKSIHFDSAWV
                     PYTNFSPIYEGKCGMSGGRVEGKVIYETQSTHKLLAAFSQASMIHVKGDVNEETFNEA
                     YMMHTTTSPHYGIVASTETAAAMMKGNAGKRLINGSIERAIKFRKEIKRLRTESDGWF
                     FDVWQPDHIDTTECWPLRSDSTWHGFKNIDNEHMYLDPIKVTLLTPGMEKDGTMSDFG
                     IPASIVAKYLDEHGIVVEKTGPYNLLFLFSIGIDKTKALSLLRALTDFKRAFDLNLRV
                     KNMLPSLYREDPEFYENMRIQELAQNIHKLIVHHNLPDLMYRAFEVLPTMVMTPYAAF
                     QKELHGMTEEVYLDEMVGRINANMILPYPPGVPLVMPGEMITEESRPVLEFLQMLCEI
                     GAHYPGFETDIHGAYRQADGRYTVKVLKEESKK"
     gene            3715..5172
                     /gene="dtpC"
                     /locus_tag="ACHOSC_RS20950"
                     /old_locus_tag="pgaptmp_004190"
     CDS             3715..5172
                     /gene="dtpC"
                     /locus_tag="ACHOSC_RS20950"
                     /old_locus_tag="pgaptmp_004190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide/tripeptide permease DtpC"
                     /protein_id="HGU4550006.1"
                     /translation="MKTPSQPRAIYYIVAIQIWEYFSFYGMRALLILYLTHQLGFDDN
                     HAISLFSAYASLVYVTPILGGWLADRLLGNRTAVIAGALLMTLGHVVLGIDTNSTFSL
                     YLALAIIICGYGLFKSNISCLLGELYDENDHRRDGGFSLLYAAGNIGSIAAPIACGLA
                     AQWYGWHVGFALAGGGMFIGLLIFLSGHRHFQSTRSMDKKALTSVKFALPVWSWLVVM
                     LCLAPVFFTLLLENDWSGYLLAIVCLIAAQIIARMMIKFPEHRRALWQIVLLMFVGTL
                     FWVLAQQGGSTISLFIDRFVNRQAFNIEVPTALFQSVNAIAVMLAGVVLAWLASPESR
                     GNSTLRVWLKFAFGLLLMACGFMLLAFDARHAAAGGQASMAVMISGLALMGFAELFID
                     PVAIAQITRLKMSGVLTGIYMLATGAVANWLAGVVAQQTTESQISGMAIAAYQRFFSQ
                     MGEWTLACVAIIVVLAFATRFLFSTPTNMIQESND"
BASE COUNT         1236 a         1264 c         1293 g         1455 t
ORIGIN      
        1 cgtgagtatt cttagcgcca gtttggtctg gtcaggaaat agttatacat catgacccgg
       61 actccaaatt caaaaatgaa attaggagaa gagcatgagt tctgccaaga agatcgggct
      121 atttgcctgt accggtgttg ttgccggtaa tatgatgggg agcggtattg cattattacc
      181 tgcgaaccta gcaagtatcg gtggtattgc tatctggggt tggattatct ctattattgg
      241 tgcaatgtcg ctggcgtatg tatatgcccg actggcaaca aaaaacccgc aacaaggtgg
      301 cccaattgct tatgccggag aaatttcccc tgcatttggt tttcagacag gtgttcttta
      361 ttaccatgct aactggattg gtaacctggc gattggtatt accgcggtat cttatctttc
      421 caccttcttc ccagtattaa atgatcctgt tccggcgggt atcgcctgta ttgctatcgt
      481 ctgggtattt acctttgtaa atatgctcgg cggtacttgg gtaagccgtt taaccactat
      541 tggtctggtg ctggttctta ttccggtagt aatgaccgct attgttggct ggcattggtt
      601 tgatgcggca acttatgcag ctaactggaa tactgcggat accactgatg gtcatgcgat
      661 cattaaaagt attctgctct gcctgtgggc cttcgtgggt gttgaatccg cagcagtaag
      721 tactggtatg gttaaaaacc cgaaacgtac cgttccgctg gcaaccatgc tgggtactgg
      781 tttagcaggt attgtttaca tcgctgcgac tcaggtgctt tccggtatgt atccatcttc
      841 tgtaatggca gcttccggtg ctccgtttgc aatcagtgct tcaaccatcc tcggtaactg
      901 ggctgcgccg ctggtttctg cattcaccgc ctttgcgtgc ctgacttctc tgggctcctg
      961 gatgatgttg gtaggccagg caggtgtacg tgccgctaac gacggtaact tcccgaaagt
     1021 ttatggtgaa gtcgacagca acggtattcc gaaaaaaggt ctgctgctgg ctgcagtgaa
     1081 aatgactgcc ctgatgatcc tcatcactct gatgaactct gccggtggta aagcatctga
     1141 cctgttcggt gaactgaccg gtatcgcagt actgctgact atgctgccgt acttctactc
     1201 ttgcgttgac ctgattcgtt ttgaaggcgt taacatccgc aactttgtca gcctgatctg
     1261 ctctgtactg ggttgtgtgt tctgcttcat cgcgctgatg ggcgcaagct ccttcgagct
     1321 ggcaggtacc ttcatcgtca gcctgattat cctgatgttc tacgctcgca aaatgcacga
     1381 gcgccagagc cactcaatgg ataaccacac cgcgtctaac gcacattaat taaaagtatt
     1441 ttccgaggct cctcctttca ttttgtccca tgtgttggga ggggcctttt ttacctggag
     1501 atatgactat gaacgttatt gcaatattga atcacatggg ggtttatttt aaagaagaac
     1561 ccatccgtga acttcatcgc gcgcttgaac gtctgaactt ccagattgtt tacccgaacg
     1621 accgtgacga cttattaaaa ctgatcgaaa acaatgcgcg tctgtgcggc gttatttttg
     1681 actgggataa atataatctc gagctgtgcg aagaaattag caaaatgaac gagaacctgc
     1741 cgttgtacgc gttcgctaat acgtattcca ctctcgatgt aagcctgaat gacctgcgtt
     1801 tacagattag cttctttgaa tatgcgctgg gtgctgctga agatattgct aataagatca
     1861 agcagaccac tgacgaatat atcaacacta ttctgcctcc gctgactaaa gcactgttta
     1921 aatatgttcg tgaaggtaaa tatactttct gtactcctgg tcacatgggc ggtactgcat
     1981 tccagaaaag cccggtaggt agcctgttct atgatttctt tggtccgaat accatgaaat
     2041 ctgatatttc catttcagta tctgaactgg gttctctgct ggatcacagt ggtccacaca
     2101 aagaagcaga acagtatatc gctcgcgtct ttaacgcaga ccgcagctac atggtgacca
     2161 acggtacttc cactgcgaac aaaattgttg gcatgtactc tgctccggca ggcagcacca
     2221 ttctgattga ccgtaactgc cacaaatcgc tgacccacct gatgatgatg agcgatgtta
     2281 cgccaatcta tttccgcccg acccgtaacg cttacggtat tcttggtggt atcccacaga
     2341 gtgaattcca gcacgctacc attgctaagc gcgtgaaaga aacaccaaac gcaacctggc
     2401 cggtacatgc tgtaattacc aactctacct atgatggtct gctgtacaac accgacttca
     2461 tcaagaaaac actggatgtg aaatccatcc actttgactc cgcgtgggtg ccttacacca
     2521 acttctcacc gatttacgaa ggtaaatgcg gtatgagcgg tggccgtgta gaagggaaag
     2581 tgatttacga aacccagtcc actcacaaac tgctggcggc gttctctcag gcttccatga
     2641 tccacgttaa aggtgacgta aacgaagaaa cctttaacga agcctacatg atgcacacca
     2701 ccacttctcc gcactacggt atcgtggcgt ccactgaaac cgctgcggcg atgatgaaag
     2761 gcaatgcagg taaacgtctg atcaacggtt ccattgaacg tgcgatcaaa ttccgtaaag
     2821 agatcaaacg tctgagaacg gaatctgatg gctggttctt tgatgtatgg cagccggatc
     2881 atatcgatac gactgaatgc tggccgctgc gttctgacag cacctggcac ggcttcaaaa
     2941 acatcgataa cgagcacatg tatcttgacc cgatcaaagt caccctgctg actccgggga
     3001 tggaaaaaga cggcaccatg agcgactttg gtattccggc cagcatcgtg gcgaaatacc
     3061 tcgacgaaca tggcatcgtt gttgagaaaa ccggtccgta taacctgctg ttcctgttca
     3121 gcatcggtat cgataagacc aaagcactga gcctgctgcg tgctctgact gacttcaaac
     3181 gtgcgttcga cctgaacctg cgtgtgaaaa acatgctgcc gtctctgtat cgtgaagatc
     3241 ctgaattcta tgaaaacatg cgtattcagg aactggctca gaatatccac aaactgattg
     3301 ttcaccacaa tctgccggat ctgatgtacc gcgcatttga agtgctgccg acgatggtaa
     3361 tgactccgta tgctgcattc cagaaagagc tgcacggtat gaccgaagaa gtttacctcg
     3421 acgaaatggt aggtcgtatt aacgccaata tgatccttcc gtatccgccg ggagttcctc
     3481 tggtaatgcc gggtgaaatg atcaccgaag aaagccgtcc ggttctggag ttcctgcaga
     3541 tgctgtgtga aatcggcgct cactatccgg gctttgaaac cgatattcac ggtgcatacc
     3601 gtcaggctga tggccgctat accgttaagg tattgaaaga agaaagcaaa aaataattag
     3661 ctcgtacaag ggaagtggct tgccacttcc cttttttgct cataaggaga acacatgaaa
     3721 acaccctcac agccgcgcgc gatatactat atcgtggcga tccaaatctg ggagtacttc
     3781 agtttttacg gcatgcgtgc cttactcatt ctctatctca cccatcagct tggttttgat
     3841 gataaccatg ccatcagcct gttcagcgca tatgcttctc tggtttacgt tacccctatt
     3901 ctcggcggct ggcttgccga ccgcctgctc ggcaaccgca ctgcagtgat tgccggcgcg
     3961 ctgttaatga cccttggcca tgtggtgctg ggcattgata caaattcaac ctttagcctg
     4021 tatctggcgc tggcaatcat tatttgtggc tacggtttat tcaaatcaaa catcagctgt
     4081 ttgcttggcg agctctacga cgagaacgat catcgacgtg atggcggttt ttcgctgctg
     4141 tatgctgcgg gcaatatcgg ttctatcgcc gccccaatcg cctgcggcct ggctgctcag
     4201 tggtatggat ggcatgttgg ctttgccctt gcgggtggcg gcatgtttat cggtttgttg
     4261 attttcttaa gcgggcatcg tcatttccag tccacgcgta gtatggataa aaaagcgctc
     4321 actagcgtga aatttgcctt accagtatgg agctggttag tggtgatgct ctgtttagcc
     4381 ccagtatttt ttaccctgct gctggagaac gactggtccg gatatttgct ggcgatcgtt
     4441 tgcctcattg ccgcacaaat cattgcccgc atgatgatca aattccccga acaccgccgt
     4501 gctctttggc aaattgtatt gttgatgttt gtcgggacgt tgttctgggt actggcacaa
     4561 cagggcggca gtaccatcag cttgtttatc gatcgctttg tgaatcgtca ggcattcaat
     4621 attgaagtac ctacagcact attccagtcg gtgaatgcaa ttgcagtgat gctcgctggg
     4681 gttgtactgg cctggctggc gtcgccagaa agccgcggca actcaacatt gcgcgtctgg
     4741 ctgaagtttg cctttggctt actgctgatg gcttgtggct ttatgttgtt ggcatttgat
     4801 gcccgacatg cagcggctgg cggtcaagcg tcaatggccg tgatgatatc cgggctggcg
     4861 ctaatgggct ttgccgaact ctttattgac ccggtggcga ttgcgcaaat cacgcgtctg
     4921 aaaatgtctg gcgtattaac cggtatttat atgctggcaa caggcgcggt cgccaactgg
     4981 ctggcaggcg tcgtggcaca gcagacgaca gagtcgcaaa ttagcggtat ggcaattgca
     5041 gcttaccagc gattcttttc tcagatggga gagtggacgt tggcttgtgt cgcgatcatc
     5101 gtcgtattgg cctttgctac ccgttttctg tttagcacgc cgacgaatat gatacaggag
     5161 agcaacgatt aactgtcgta aaaacaattg gttatgaata aattaacttt cagtttataa
     5221 cactagcaaa tctagattaa taagtcat
//
LOCUS       DAZKHF020000278         5205 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2201S31241
            SAMN43267800-rid25106413.denovo.280, whole genome shotgun sequence.
ACCESSION   DAZKHF020000278 DAZKHF020000000
VERSION     DAZKHF020000278.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267800
            Sequence Read Archive: SRR30307263
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5205)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5205)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 5205)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350581.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350581.2
            Genome Coverage       :: 151x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:21:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,652
            CDSs (total)                      :: 5,557
            Genes (coding)                    :: 5,320
            CDSs (with protein)               :: 5,320
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 237
            CDSs (without protein)            :: 237
            Pseudo Genes (ambiguous residues) :: 0 of 237
            Pseudo Genes (frameshifted)       :: 102 of 237
            Pseudo Genes (incomplete)         :: 130 of 237
            Pseudo Genes (internal stop)      :: 52 of 237
            Pseudo Genes (multiple problems)  :: 41 of 237
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5205
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267800-rid25106413.denovo.280"
                     /serotype="O 26:H11"
                     /isolate="2201S31241"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-01"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            <1..342
                     /gene="lpfD-O113"
                     /locus_tag="ACHOSC_RS20955"
                     /old_locus_tag="pgaptmp_004191"
     CDS             <1..342
                     /gene="lpfD-O113"
                     /locus_tag="ACHOSC_RS20955"
                     /old_locus_tag="pgaptmp_004191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001685709.1"
                     /note="LpfA-O113 is the major subunit of a form of long
                     polar fimbria first described in LEE-negative, Shiga
                     toxin-producing enterohemorrhagic Escherichia coli (STEC)
                     of serotype O113:H21. This protein, designated LpfD-O113,
                     is the minor subunit of this chaperone-usher type fimbrial
                     system; Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long polar fimbrial minor subunit LpfD-O113"
                     /protein_id="HGU4550007.1"
                     /translation="THKITKTVTIECENLGILNTDDISTSFYATEPNTDNSMVVTSNS
                     NVGIKIYDKNNKEIKVNGGELPTDMGKSTVYGEKSGSVTFSAAPASLTGARPSPGQFT
                     ATATITVEIVR"
     gene            590..1630
                     /gene="pstS"
                     /locus_tag="ACHOSC_RS20960"
                     /old_locus_tag="pgaptmp_004192"
     CDS             590..1630
                     /gene="pstS"
                     /locus_tag="ACHOSC_RS20960"
                     /old_locus_tag="pgaptmp_004192"
                     /EC_number="7.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0042301 - phosphate ion binding [Evidence
                     IEA];
                     GO_process: GO:0035435 - phosphate ion transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter substrate-binding
                     protein PstS"
                     /protein_id="HGU4550008.1"
                     /translation="MKVMRTTVATVVAATLSMSAFSVFAEASLTGAGATFPAPVYAKW
                     ADTYQKETGNKVNYQGIGSSGGVKQIIANTVDFGASDAPLSDEKLAQEGLFQFPTVIG
                     GVVLAVNIPGLKSGELVLDGKTLGDIYLGKIKKWDDEAIAKLNPGLKLPSQNIAVVRR
                     ADGSGTSFVFTSYLAKVNEEWKNNVGTGSTVKWPIGLGGKGNDGIAAFVQRLPGAIGY
                     VEYAYAKQNNLAYTKLISADGKPVSPTEENFANAAKGADWSKTFAQDLTNQKGEDAWP
                     ITSTTFILIHKDQKKPEQGTEVLKFFDWAYKTGAKQANDLDYASLPDSVVEQVRAAWK
                     TNIKDSSGKPLY"
     gene            1717..2676
                     /gene="pstC"
                     /locus_tag="ACHOSC_RS20965"
                     /old_locus_tag="pgaptmp_004193"
     CDS             1717..2676
                     /gene="pstC"
                     /locus_tag="ACHOSC_RS20965"
                     /old_locus_tag="pgaptmp_004193"
                     /EC_number="7.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008806729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006817 - phosphate ion transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease PstC"
                     /protein_id="HGU4550009.1"
                     /translation="MAATKPAFNPPGKKGDIIFSVLVKLAALIVLLMLGGIIVSLIIS
                     SWPSIQKFGLAFLWTKEWDAPNDIYGALVPIYGTLVTSFIALLIAVPVSFGIALFLTE
                     LAPGWLKRPLGIAIELLAAIPSIVYGMWGLFIFAPLFAVYFQEPVGNIMSNIPIVGAL
                     FSGPAFGIGILAAGVILAIMIIPYIAAVMRDVFEQTPVMMKESAYGIGCTTWEVIWRI
                     VLPFTKNGVIGGIMLGLGRALGETMAVTFIIGNTYQLDSASLYMPGNSITSALANEFA
                     EAESGLHVAALMELGLILFVITFIVLAASKFMIMRLAKNEGAR"
     gene            2676..3566
                     /gene="pstA"
                     /locus_tag="ACHOSC_RS20970"
                     /old_locus_tag="pgaptmp_004194"
     CDS             2676..3566
                     /gene="pstA"
                     /locus_tag="ACHOSC_RS20970"
                     /old_locus_tag="pgaptmp_004194"
                     /EC_number="7.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0035435 - phosphate ion transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease PstA"
                     /protein_id="HGU4550010.1"
                     /translation="MAMVEMQTTAALAESRRKMQARRRLKNRIALTLSMATMAFGLFW
                     LIWILMSTITRGIDGMSLALFTEMTPPPNTEGGGLANALAGSGLLILWATVFGTPLGI
                     MAGIYLAEYGRKSWLAEVIRFINDILLSAPSIVVGLFVYTIVVAQMEHFSGWAGVIAL
                     ALLQVPIVIRTTENMLKLVPDSLREAAYALGTPKWKMISAITLKASVSGIMTGILLAI
                     ARIAGETAPLLFTALSNQFWSTDMMQPIANLPVTIFKFAMSPFAEWQQLAWAGVLIIT
                     LCVLLLNILARVVFAKNKHG"
     gene            3657..4430
                     /gene="pstB"
                     /locus_tag="ACHOSC_RS20975"
                     /old_locus_tag="pgaptmp_004195"
     CDS             3657..4430
                     /gene="pstB"
                     /locus_tag="ACHOSC_RS20975"
                     /old_locus_tag="pgaptmp_004195"
                     /EC_number="7.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000063125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0006817 - phosphate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter ATP-binding protein
                     PstB"
                     /protein_id="HGU4550011.1"
                     /translation="MSMVETAPSKIQVRNLNFYYGKFHALKNINLDIAKNQVTAFIGP
                     SGCGKSTLLRTFNKMFELYPEQRAEGEILLDGDNILTNSQDIALLRAKVGMVFQKPTP
                     FPMSIYDNIAFGVRLFEKLSRADMDERVQWALTKAALWNETKDKLHQSGYSLSGGQQQ
                     RLCIARGIAIRPEVLLLDEPCSALDPISTGRIEELITELKQDYTVVIVTHNMQQAARC
                     SDHTAFMYLGELIEFSNTDDLFTKPAKKQTEDYITGRYG"
     gene            4445..5170
                     /gene="phoU"
                     /locus_tag="ACHOSC_RS20980"
                     /old_locus_tag="pgaptmp_004196"
     CDS             4445..5170
                     /gene="phoU"
                     /locus_tag="ACHOSC_RS20980"
                     /old_locus_tag="pgaptmp_004196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0030643 - intracellular phosphate ion
                     homeostasis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate signaling complex protein PhoU"
                     /protein_id="HGU4550012.1"
                     /translation="MDSLNLNKHISGQFNAELESIRTQVMTMGGMVEQQLSDAITAMH
                     NQDSDLAKRVIEGDKNVNMMEVAIDEACVRIIAKRQPTASDLRLVMVISKTIAELERI
                     GDVADKICRTALEKFSQQHQPLLVSLESLGRHTIQMLHDVLDAFARMDIDEAVRIYRE
                     DKKVDQEYEGIVRQLMTYMMEDSRTIPSVLTALFCARSIERIGDRCQNICEFIFYYVK
                     GQDFRHVGGDELDKLLAGKDSDK"
BASE COUNT         1248 a         1284 c         1402 g         1271 t
ORIGIN      
        1 acacataaaa ttactaaaac ggtcactatt gagtgtgaaa acctggggat actgaatacc
       61 gatgatatca gtacgtcttt ttatgctacc gaacccaata cggacaattc aatggtcgta
      121 acttcaaact ctaacgtggg gataaaaatt tacgacaaaa ataataagga aatcaaagtg
      181 aacggtggtg agttgccaac agacatgggt aaatcaacag tgtatggtga aaaatcaggt
      241 agtgtaactt tttcagctgc tcctgcgagc cttactggag ctcgcccatc cccggggcaa
      301 tttactgcaa cagcgacgat aactgttgag attgtgcgct aataggtctg acaacgattg
      361 ttcatcacat cgttgtcatc tcacccattt ctttatcact tttaaatgca tcatgacaaa
      421 aagatgacat gtgggatttt catcttcaca tttaaaatca gtaagttatt tggtaattcc
      481 tttctgtcat aaaaatgtca tattccttac atataactgt cacctgtttg tcctattttg
      541 cttctcgtag ccaacaaaca atgctttatg aatcctccca ggagacatta tgaaagttat
      601 gcgtaccacc gtcgcaactg ttgtcgccgc gaccttatcg atgagcgctt tctctgtgtt
      661 tgcagaagca agcctgacag gtgcaggtgc aaccttccct gcgccggtgt atgccaaatg
      721 ggctgacact taccagaaag aaaccggtaa taaagttaac taccagggta tcggttcttc
      781 cggtggcgta aaacagatta tcgctaatac cgttgatttt ggtgcctctg acgcgccgct
      841 gtctgacgaa aaactggctc aggaaggtct gttccagttc ccgaccgtga ttggcggcgt
      901 ggtgctggcg gttaacattc cagggctgaa gtctggcgaa ctggtgctgg atggtaaaac
      961 cctcggcgac atctacctgg gcaaaatcaa gaagtgggat gatgaagcca tcgccaaact
     1021 gaatccgggt ctgaaactgc cttcacaaaa cattgctgta gtacgccgcg cagatggctc
     1081 cgggacttcc ttcgtcttca ccagctacct ggcgaaagtg aacgaagagt ggaaaaacaa
     1141 cgttggtact ggctctaccg taaaatggcc gatcggtctg ggcggtaaag gtaacgacgg
     1201 tatcgccgcg ttcgttcagc gtctgccggg tgcaattggt tatgttgaat atgcttacgc
     1261 gaagcagaac aacctggcgt acaccaaact gatctccgct gatggtaaac cggttagtcc
     1321 gaccgaagaa aacttcgcta atgcagcaaa aggtgcagac tggagcaaaa ccttcgctca
     1381 ggatctgacc aaccagaaag gcgaagatgc atggcctatt acctctacca cgttcattct
     1441 gatccacaaa gatcagaaga aaccagaaca aggcacagaa gtgctgaaat tcttcgactg
     1501 ggcgtacaaa accggggcta aacaggcgaa cgacctggat tacgccagcc tgccggatag
     1561 tgtagttgaa caggttcgcg ctgcgtggaa gaccaatatt aaagacagta gcggtaagcc
     1621 gctgtactaa taaaactcca ggccgggtac ggtgttttac gccgcatccg gcattacaaa
     1681 atgactttgt aaacgcgttt aactgaagag taacttatgg ctgcaaccaa gcctgctttt
     1741 aacccaccgg gtaaaaaggg cgacataatt ttcagcgtgc tggtaaaact ggcggcgctg
     1801 attgtgctat tgatgttggg tggcattatt gtctctctga tcatctcctc ctggccgagc
     1861 attcagaaat ttggtctggc tttcctatgg accaaagagt gggatgcacc gaacgatatc
     1921 tacggggcgc tggtgccgat ctacggtacg ttggtgactt cgtttatcgc gctgctgatc
     1981 gccgtcccgg tgagtttcgg tatcgccctg ttcctgactg agcttgcgcc tggctggctg
     2041 aaacgcccgc tgggtatcgc cattgagctg ctggcagcca ttccaagtat cgtttacggc
     2101 atgtggggcc tgtttatctt tgcgccgctg ttcgccgttt actttcagga gccggtcggc
     2161 aatatcatgt cgaatatccc gattgttggc gcgctgttct ctggccccgc atttggtatc
     2221 ggtatcctcg cggcaggcgt gatcctcgcc atcatgatta ttccgtacat tgcggcggta
     2281 atgcgtgatg tgttcgaaca aaccccggtg atgatgaaag agtcggccta cggtattggc
     2341 tgcaccacct gggaagttat ctggcgtatc gttcttccgt tcaccaaaaa tggtgttatc
     2401 ggcggcatca tgctggggct gggccgcgcg ctcggtgaaa ccatggcggt gacctttatc
     2461 atcggtaaca cctaccagct cgacagcgcc tcgctgtata tgccgggcaa cagtatcacc
     2521 tctgcgctgg cgaacgaatt tgcggaagcg gaatccggtc tgcacgttgc agcactgatg
     2581 gaactgggcc tgatcctgtt tgtgattacc ttcatcgtcc tcgccgcatc gaagtttatg
     2641 attatgcgcc tggctaagaa tgagggggca cgctaatggc tatggttgaa atgcaaacca
     2701 ctgcggcgct ggctgaatct cgccgcaaaa tgcaggcgcg tcgccgcctc aaaaaccgta
     2761 ttgcgctgac gctctcgatg gcgacgatgg ccttcggcct gttctggctg atctggattt
     2821 taatgtccac catcactcgc ggtatcgacg gtatgtcgct ggcgctgttc actgaaatga
     2881 cgccgccgcc caatacggaa ggtggtggtc tggcgaacgc cctggcgggt agcgggctgt
     2941 taattttgtg ggccacggta ttcggtacgc cgctgggcat tatggcgggg atttatctgg
     3001 cggaatatgg tcgtaaatcc tggctggcag aagtgattcg cttcattaac gacattctgc
     3061 tctccgcgcc gtcgattgtg gttggtctgt ttgtctacac catcgtggtg gcacagatgg
     3121 agcacttctc cggctgggcg ggcgtaattg cgctggcgct gttgcaggtg ccgattgtta
     3181 tccgcaccac cgagaacatg ctgaaactgg tgccggacag cctgcgtgaa gcggcttatg
     3241 cgctgggtac accgaagtgg aagatgatct ccgcgattac gctgaaagcg tcggtgtccg
     3301 ggatcatgac cggtatcttg ctggcgattg cccgtattgc tggtgaaacc gcgccgctgc
     3361 tgtttaccgc gctctccaac cagttctgga gcacggacat gatgcagccg atcgccaacc
     3421 tgccggtgac gatctttaag tttgcgatga gtccgtttgc ggaatggcag caactggcct
     3481 gggccggggt attgatcatt accctgtgcg tactgctgct gaacattctg gcgcgcgttg
     3541 tttttgcgaa gaataaacac ggttaacact ttgccggatg cggcgtgaac gcctgatccg
     3601 gcctacggta agcctgatta gcgaagtgca tcaggcacga tgaggaaaag attgcaatga
     3661 gtatggttga aactgccccg agtaaaattc aggttcgtaa tttgaacttc tactacggca
     3721 aattccatgc cctgaaaaac atcaacctgg atatcgctaa aaaccaggta acggcgttta
     3781 tcgggccgtc cggctgcggt aaatcgacgc tgctgcgtac cttcaacaaa atgtttgaac
     3841 tgtacccgga gcagcgtgcg gaaggtgaaa ttctgcttga tggcgacaac atcctgacca
     3901 actctcagga tatcgccctg ctgcgtgcga aagtgggcat ggtgttccag aaaccgacgc
     3961 cgtttccgat gtccatctac gacaacatcg cttttggcgt tcgtctgttt gagaagctct
     4021 cccgtgccga catggacgag cgcgtgcagt gggcattgac caaagccgca ttgtggaacg
     4081 aaaccaaaga taaattgcac cagagcggtt actctctctc tggtggtcag caacagcgtc
     4141 tgtgtattgc gcgtggtatc gccattcgcc cggaagtgct gctgctcgac gaaccgtgtt
     4201 cggcgctcga ccctatctct accgggcgta ttgaagagct gatcaccgaa ctgaagcagg
     4261 attacaccgt ggtgatcgtc acccacaaca tgcagcaggc tgcgcgttgt tccgaccaca
     4321 cggcgtttat gtacctgggc gaattgattg agttcagcaa cacggacgat ctgttcacca
     4381 agccagcgaa gaaacaaaca gaagactaca tcaccggtcg ttacggttga ttcaggagtg
     4441 cgttatggac agtctcaatc ttaataaaca tatttccggc cagttcaacg ccgaactgga
     4501 aagtatccgc acgcaggtga tgaccatggg cggcatggtg gagcagcagc tttctgatgc
     4561 aatcaccgcg atgcataacc aggacagcga tctggcgaag cgcgtgatcg aaggcgacaa
     4621 gaacgtcaac atgatggaag tggcgatcga tgaagcctgc gtgcgcatta tcgccaaacg
     4681 tcagccgacg gcgagcgacc tgcgactggt tatggtgatc agtaaaacca ttgccgagct
     4741 ggagcgtatt ggcgacgtgg cggacaaaat ctgccgtact gcgctggaga aattctccca
     4801 gcagcatcag ccgttgctgg taagtctgga gtcgctgggc cgtcatacca tccagatgct
     4861 gcacgacgtg ctggacgcgt tcgcgcggat ggacattgac gaagcggtac gtatttatcg
     4921 tgaagataaa aaagtcgatc aggaatacga aggtattgtt cgtcaactga tgacctacat
     4981 gatggaagat tcgcgtacca ttccgagcgt acttactgcg ctgttctgcg cgcgttctat
     5041 cgaacgtatt ggcgaccgct gccagaatat ttgtgagttt atcttctact acgtgaaggg
     5101 gcaggatttc cgtcatgtag gcggtgatga gctggataaa ctgctggcgg gaaaagatag
     5161 cgacaaataa ttcaccagac aaatcccaat aacttaatta ttggg
//
LOCUS       DAZKHF020000279         5202 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2201S31241
            SAMN43267800-rid25106413.denovo.281, whole genome shotgun sequence.
ACCESSION   DAZKHF020000279 DAZKHF020000000
VERSION     DAZKHF020000279.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267800
            Sequence Read Archive: SRR30307263
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5202)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5202)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 5202)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350581.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350581.2
            Genome Coverage       :: 151x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:21:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,652
            CDSs (total)                      :: 5,557
            Genes (coding)                    :: 5,320
            CDSs (with protein)               :: 5,320
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 237
            CDSs (without protein)            :: 237
            Pseudo Genes (ambiguous residues) :: 0 of 237
            Pseudo Genes (frameshifted)       :: 102 of 237
            Pseudo Genes (incomplete)         :: 130 of 237
            Pseudo Genes (internal stop)      :: 52 of 237
            Pseudo Genes (multiple problems)  :: 41 of 237
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5202
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267800-rid25106413.denovo.281"
                     /serotype="O 26:H11"
                     /isolate="2201S31241"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-01"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            <1..215
                     /locus_tag="ACHOSC_RS20985"
                     /old_locus_tag="pgaptmp_004197"
     CDS             <1..215
                     /locus_tag="ACHOSC_RS20985"
                     /old_locus_tag="pgaptmp_004197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="sialate O-acetylesterase"
                     /protein_id="HGU4550013.1"
                     /translation="VDFQQQGERGLTNAPDEDPDDLSTGYYGSAYRSPENWTTALRSS
                     HFSTAARRGIISDKFVEAILQFWRER"
     gene            complement(223..873)
                     /locus_tag="ACHOSC_RS20990"
                     /old_locus_tag="pgaptmp_004198"
     CDS             complement(223..873)
                     /locus_tag="ACHOSC_RS20990"
                     /old_locus_tag="pgaptmp_004198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001586610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HGU4550014.1"
                     /translation="MNKLPDHLPRRYIIEAIHLWDAGETHQFQPARLYEIEYHGRRYP
                     SKAIAGIAATLMTGTQFTPADFTGGIKSKCVKLLIEQGFHIHLDKAAAAPIADVLFPD
                     ELPSQETYLEGAAIKVTVNRYERDKKARDKAVEHHGCQCNVCGVDLVKIYGDIAEGFI
                     HVHHLVPLSAIKEDYQLDPVNDLLPVYPNCHAMLHRRKPPFTPEQLKALMDANKSN"
     gene            1010..1153
                     /locus_tag="ACHOSC_RS20995"
                     /old_locus_tag="pgaptmp_004199"
                     /pseudo
     CDS             1010..1153
                     /locus_tag="ACHOSC_RS20995"
                     /old_locus_tag="pgaptmp_004199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_080685909.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease"
     gene            complement(1817..1945)
                     /locus_tag="ACHOSC_RS21000"
                     /old_locus_tag="pgaptmp_004200"
                     /pseudo
     CDS             complement(1817..1945)
                     /locus_tag="ACHOSC_RS21000"
                     /old_locus_tag="pgaptmp_004200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005166249.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
     gene            complement(1921..2070)
                     /locus_tag="ACHOSC_RS21005"
                     /old_locus_tag="pgaptmp_004201"
                     /pseudo
     CDS             complement(1921..2070)
                     /locus_tag="ACHOSC_RS21005"
                     /old_locus_tag="pgaptmp_004201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309436.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease subunit M"
     gene            complement(2155..2352)
                     /locus_tag="ACHOSC_RS21010"
                     /old_locus_tag="pgaptmp_004202"
     CDS             complement(2155..2352)
                     /locus_tag="ACHOSC_RS21010"
                     /old_locus_tag="pgaptmp_004202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF957 domain-containing protein"
                     /protein_id="HGU4550015.1"
                     /translation="MKSLTTETALDILIAWLQDNIDCESGIIFDNNEDKTDSAALLPC
                     IEQAREDIRTLRQLQLQHQNR"
     gene            complement(2364..2852)
                     /locus_tag="ACHOSC_RS21015"
                     /old_locus_tag="pgaptmp_004203"
     CDS             complement(2364..2852)
                     /locus_tag="ACHOSC_RS21015"
                     /old_locus_tag="pgaptmp_004203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001499210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5983 family protein"
                     /protein_id="HGU4550016.1"
                     /translation="MKLSLMLEADRINVQALNMGRIVVDVDGVNLTELINKVAENGYS
                     LRVVEESDQQSTCTLPPFATLAGIRCSTAHITEKDNAWLYSLSHQTSDVGESEWIHFT
                     GSGYLLRTDAWSYPVLRLKRLGLSKTFRRLVITLTRRYGVSLIHLDASAECLPGLPTF
                     NW"
     gene            complement(2849..3223)
                     /locus_tag="ACHOSC_RS21020"
                     /old_locus_tag="pgaptmp_004204"
     CDS             complement(2849..3223)
                     /locus_tag="ACHOSC_RS21020"
                     /old_locus_tag="pgaptmp_004204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TA system toxin CbtA family protein"
                     /protein_id="HGU4550017.1"
                     /translation="MKTLPDTHVREASSCPSPVTIWQTLLSRLLGQHYGLTLNDTPFA
                     DERVIEQHIEAGISLCDAVNFLVEKYALVRTDQPGFSTCPRSQLINSIDILRARRATG
                     LMTRDNYRTVNNITLGKHPEKR"
     gene            complement(3313..3681)
                     /locus_tag="ACHOSC_RS21025"
                     /old_locus_tag="pgaptmp_004205"
     CDS             complement(3313..3681)
                     /locus_tag="ACHOSC_RS21025"
                     /old_locus_tag="pgaptmp_004205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001280950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV toxin-antitoxin system YeeU family
                     antitoxin"
                     /protein_id="HGU4550018.1"
                     /translation="MSDTLSGTTHPDDNDDHPWWGLPCTVTPCFGARLVQEGNRLHYL
                     ADRAGIRGLFSDADAYHLDQAFPLLMKQLELMLTSGELNPRHQHTVTLYAKGLTCKAD
                     TLGSGGYVYLAVYPMPETKK"
     gene            complement(3844..4065)
                     /locus_tag="ACHOSC_RS21030"
                     /old_locus_tag="pgaptmp_004206"
     CDS             complement(3844..4065)
                     /locus_tag="ACHOSC_RS21030"
                     /old_locus_tag="pgaptmp_004206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF987 domain-containing protein"
                     /protein_id="HGU4550019.1"
                     /translation="MKIITRGEAMRIHRQHPASRLFPFCTGKYRWHGSAEAYTGREVQ
                     DIPGVLAVFAERRKDSFGPYVRLMSVTLN"
     gene            complement(4128..4604)
                     /locus_tag="ACHOSC_RS21035"
                     /old_locus_tag="pgaptmp_004207"
     CDS             complement(4128..4604)
                     /locus_tag="ACHOSC_RS21035"
                     /old_locus_tag="pgaptmp_004207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RadC family protein"
                     /protein_id="HGU4550020.1"
                     /translation="MQQLSFLPGEMTPGERSLIQRALKTLDRHLHEPGVAFTSTRAAR
                     EWLILNMAGLEREEFRVLYLNNQNQLIAGETLFTGTINRTEVHPREVIKRALYHNAAA
                     VVLAHNHPSGEVTPSKADRLITERLVQALGLVDIRVPDHLIVGGSQVFSFAEHGLL"
     gene            complement(4620..5105)
                     /locus_tag="ACHOSC_RS21040"
                     /old_locus_tag="pgaptmp_004208"
     CDS             complement(4620..5105)
                     /locus_tag="ACHOSC_RS21040"
                     /old_locus_tag="pgaptmp_004208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antirestriction protein"
                     /protein_id="HGU4550021.1"
                     /translation="MKTISHNSTTSSVSVTAASGNDQPQLVATLVPDEQRISFWPQHF
                     GLIPQWVTLEPRVFGWMDRLCEDYCGGIWNLYTLNNGGAFMAPEPDDDDDETWVLFNA
                     MNGNRAEMSPEAAGIAACLMTYSHHACRTECYAMTVHYYRLRDYALQHPECSAIMRII
                     D"
BASE COUNT         1315 a         1249 c         1370 g         1268 t
ORIGIN      
        1 tcgtcgactt ccagcaacaa ggtgaaagag gactgacgaa cgcgcctgat gaagacccgg
       61 acgatttaag cacgggatat tacggttcag cgtaccggtc accggagaac tggacgacgg
      121 cactgcgaag cagtcatttc agcacggcag cccgtcgggg gattatttct gacaagtttg
      181 tagaagcaat tttgcagttt tggcgcgaaa ggtgagcgtt cattaatttg atttattcgc
      241 atccatcagc gcttttaact gctccggcgt aaacggtggt ttgcgtcggt gcaacatcgc
      301 atggcaatta gggtacacag gcagcaaatc attaactgga tccagctggt aatcctcttt
      361 aatggctgac agcggcacca ggtgatgcac atggataaag ccttcagcga tatcaccata
      421 gatcttcacc agatcaacac cgcatacgtt gcactggcat ccatgatgtt cgaccgcttt
      481 atctcgggcc tttttatcgc gttcatagcg gtttactgtc acctttatcg ccgcaccttc
      541 aaggtatgtc tcctgcgacg gcaactcatc cgggaacaga acgtcggcta tgggagccgc
      601 agcggcttta tccagatgaa tatgaaatcc ctgctctatg agtagcttga cacatttaga
      661 ttttattccg ccggtgaaat ccgcaggggt aaattgcgtc cccgtcatca atgtcgctgc
      721 gatgccagca atcgccttcg aggggtaacg tcggccatga tattcaatct catacaaccg
      781 cgcaggctgg aactgatgag tttctcccgc atcccataaa tggatagctt cgatgatata
      841 ccgccgcgga agatgatcag ggagtttatt catggagaat gtccttatac gttctcagaa
      901 caggcagcaa aaaaacaaat gagatcaagg taatatatct tcacttgcag gcttgaacag
      961 cagaaaattt gcgagcatga tcgcaattat ttcactgcca gggatatcaa tagcttccag
     1021 taaatcgcta cagcaggcaa ttgccaatat aaaaatctgg cacaagggtg aacagcgcgc
     1081 gccgcataag ccattgttat tgctatacgt attagcggga tacctgaatg gacatccgcg
     1141 ccttttcgat tattgtagtg gaacagtcta cgaggatgca gttcgaagtg agtaagtaaa
     1201 ggttgcggga aacgatcaat gccgccatac aatgattttt taggatcaac aatgagtaat
     1261 aaaaactacg aaagtcaccg caaagcgatt gttagtaaag gtataccacc cgctctatta
     1321 aataggctca ccaattcgga tgttcaggtg atcaatacct tcttaacacg agtgagcaaa
     1381 ctggaactat ctcaacaaga gaaagactgg atcataaaaa tcatctctat ggtttagaca
     1441 atttcagcct tgtagccttt caagggtttt gtgtgggtac aaacattatc gaatcggggt
     1501 atagccgcag ctgtagctgt atccacatca gtaagttgat gtaggttgac ttaggttgag
     1561 cgaaaaatag cgaaaagcct tgtgtggcgc ggatttcagt cataaaaaaa cgtccgttga
     1621 tgtctattga tgttccgatg gtgcgaaggt gcgaaggtgc gaaggccgga ctcaaacatg
     1681 tgacttaacc tcatgattta aaattgttaa taataaacaa tatcatctta taccaacaca
     1741 gataccaaca cgaaaaatca ctggttttcg gcatcgaacc aggacaatca gctcctgatt
     1801 gagataccga gctctgttac tcgtcatctt cctcgacagg cttcattgca tcaagtagta
     1861 ctcgcaccat cgcgatagaa ccaatccgga taaaagcatc ttctccatct atatctgtta
     1921 actgatatcg ctcacttgat gccggataat tgtgtcgatt tgcagcagtg aactctttcg
     1981 tacagccaga atgcggcgga tgctggttga gcagataacg tccgtattct cgttgaccac
     2041 ttccggacgg taggctgtga tatcagacat aatcgatatt cctttttgca gataaatgaa
     2101 aatgccagcc ctgttcaggc tggcgtcagt ggaatgaagt ctggtgagta agactcaccg
     2161 gttctggtgc tgaagctgca gttggcgcag ggtacggata tcctctctgg cctgttcgat
     2221 acagggcaac agtgctgctg aatccgtttt atcctcattg ttgtcaaaga taattcccga
     2281 ttcgcagtcg atattgtcct gcagccacgc aatcagaata tccagtgcgg tttccgtggt
     2341 taatgatttc atgttgttcc tggttaccag ttgaaagtgg gtaaacccgg caggcattca
     2401 gcgctggcat ccagatgaat gagactgacg ccataacgtc gggtaagtgt gataaccaga
     2461 cgacggaacg tttttgacag ccccaggcgt ttaagccgca gaaccggata tgaccacgca
     2521 tcggtacgta acagatatcc gctacctgtg aaatgaatcc attctgattc accaacgtca
     2581 ctggtctgat gcgacagcga gtacagccag gcgttatcct tttccgtgat atgtgcggta
     2641 ctgcagcgta tgccggcaag ggttgcaaac ggtggtagtg tgcaggttga ctgttggtcg
     2701 gattcctcca ccacgcggag tgaataaccg ttttcagcga ccttgttaat cagttcagtg
     2761 agattaacac catcgacgtc aacgacaatt cgccccatgt tcagtgcctg cacattaatt
     2821 ctgtcggctt ccagcatcag ggaaagtttc atcgtttctc cggatgctta cctagggtaa
     2881 tgttatttac cgttctgtaa ttgtcgcggg tcatcaggcc ggtcgccctg cgagcccgga
     2941 ggatatcaat actgtttatt aactgagagc ggggacaagt gctgaatccc ggctggtcgg
     3001 tacgcaccag cgcgtatttt tccacgagaa aattcaccgc atcacacagt gaaatgccag
     3061 cttcaatatg ctgctcaatc acacgttcat cggcgaacgg tgtgtcattc agtgtaaggc
     3121 cgtaatgctg gcccagtagt cgggagagca gagtctgcca gatggtgacg ggagacgggc
     3181 agctcgatgc ctcccgtacg tgtgtatcgg gtaatgtttt catactgaag gttttcctga
     3241 tatgcagaga tgaaaaatgg aaaagtggcg tggtgaaagc accaggccgg agcagaaggt
     3301 tattccggtg gtttactttt tcgtttcggg catcggataa acagccagat aaacgtaacc
     3361 accgctaccg agagtgtcag ctttgcaggt cagacctttt gcatacagcg tgacggtatg
     3421 ctgatggcgg ggattcagtt caccgctggt gagcatgagt tccagttgtt tcatcagcag
     3481 cggaaaggcc tggtccaggt ggtacgcatc tgcatcgctg aacaggcctc tgataccggc
     3541 gcggtcggca aggtaatgca ggcggttacc ctcctgcacc agacgggcgc cgaaacaggg
     3601 cgtcactgtg cagggtagtc cccaccaggg atggtcgtca ttgtcatcgg gatgcgttgt
     3661 cccggaaagt gtgtctgaca cgataaaatc ctcacagaaa atcggtgaat gatagtttaa
     3721 cgatgacggg tcagccggcg cgcagaccgg cttcggtaaa ggtgtattcg ttgatgagca
     3781 ggacctcatc cacagcggcg tctgagctca gccagtcgta ctcgttttcc gggtgacgga
     3841 acatcagttc agggtgacgc tcatcagccg gacatacgga ccaaaactgt ccttacggcg
     3901 ttctgcaaac acggccagca caccgggaat atcctgcact tcacgaccgg tatacgcttc
     3961 agcactgccg tgccagcggt atttgccggt acagaacgga aaaagacggg atgccggatg
     4021 ctgacggtga atacgcatgg cttcgccacg ggtgatgatt ttcatagtgg gatacctctg
     4081 aagacagaag ataaaagtga aaacaggtgt gatgtggttg tgacgggtta aagcagacca
     4141 tgttcggcaa aggagaaaac ctggctgcca ccgactatca gatggtccgg cacccggata
     4201 tccaccaggc ccagtgcctg taccagacgt tccgtgataa ggcggtctgc cttgctgggt
     4261 gtgacttcac cggacgggtg attgtgtgcc agcaccacag cagcggcatt gtgatacagg
     4321 gcgcgtttaa tcacttcacg gggatggact tccgtgcggt tgatggtgcc ggtgaagagg
     4381 gtttcaccgg caatcagctg attctggttg ttcagataca gcacccggaa ctcttcacgc
     4441 tccagtcccg ccatgttcag aatcagccat tctcgtgccg cacgggtgga ggtgaaggcc
     4501 acgccgggtt catgaagatg gcggtccagg gttttcaggg cccgttgaat gaggctgcgc
     4561 tcgccgggcg tcatctctcc gggcagaaag gaaagctgtt gcattgtact tctctccatt
     4621 cagtcgatga tgcgcataat ggcgctgcat tccggatgct gcagggcgta atcccgcagc
     4681 cggtaataat ggaccgtcat ggcataacac tccgtacgac aggcatgatg actgtacgtc
     4741 atcagacagg cagcaatgcc ggcagcttcc ggactcattt cagcgcggtt accgttcatg
     4801 gcattgaaca gtacccatgt ttcgtcatca tcgtcatccg gttcgggtgc cataaatgcc
     4861 ccgccgttgt tcagggtgta cagattccag ataccaccgc agtagtcttc acacagacgg
     4921 tccatccagc cgaagacacg gggctccagg gtcacccact gtggaatgag gccaaagtgc
     4981 tgcggccaga agctgatgcg ctgttcatca gggacgaggg tggcaaccaa ctgaggctgg
     5041 tcattccctg atgcagcggt tacggaaaca gaagatgtgg tggaattatg tgaaatggtt
     5101 ttcatgaaat gactccttaa aggacaaata aatgcgtggt tttcagggta atggctgaat
     5161 cagcgcaggc tctccagcag ggtttccgcc atcacccaca at
//
LOCUS       DAZKHF020000280         5179 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2201S31241
            SAMN43267800-rid25106413.denovo.282, whole genome shotgun sequence.
ACCESSION   DAZKHF020000280 DAZKHF020000000
VERSION     DAZKHF020000280.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267800
            Sequence Read Archive: SRR30307263
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5179)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5179)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 5179)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350581.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350581.2
            Genome Coverage       :: 151x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:21:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,652
            CDSs (total)                      :: 5,557
            Genes (coding)                    :: 5,320
            CDSs (with protein)               :: 5,320
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 237
            CDSs (without protein)            :: 237
            Pseudo Genes (ambiguous residues) :: 0 of 237
            Pseudo Genes (frameshifted)       :: 102 of 237
            Pseudo Genes (incomplete)         :: 130 of 237
            Pseudo Genes (internal stop)      :: 52 of 237
            Pseudo Genes (multiple problems)  :: 41 of 237
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5179
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267800-rid25106413.denovo.282"
                     /serotype="O 26:H11"
                     /isolate="2201S31241"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-01"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            <1..437
                     /gene="hpaR"
                     /locus_tag="ACHOSC_RS21045"
                     /old_locus_tag="pgaptmp_004209"
     CDS             <1..437
                     /gene="hpaR"
                     /locus_tag="ACHOSC_RS21045"
                     /old_locus_tag="pgaptmp_004209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="homoprotocatechuate degradation operon regulator
                     HpaR"
                     /protein_id="HGU4550022.1"
                     /translation="LTIALLQAREAAMSYFRPIVKRHNLTEQQWRIVRILAESPSMDF
                     HDLAYRACILRPSLTGILTRMERDGLVLRLKPINDQRKLYISLTKEGQALYNRAQTQI
                     EEAYRQIEAQFTAEKMQQLTHLLEEFIALGNSRQEDIPGDNE"
     gene            556..2220
                     /gene="tsr"
                     /locus_tag="ACHOSC_RS21050"
                     /old_locus_tag="pgaptmp_004210"
     CDS             556..2220
                     /gene="tsr"
                     /locus_tag="ACHOSC_RS21050"
                     /old_locus_tag="pgaptmp_004210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004888 - transmembrane signaling receptor
                     activity [Evidence IEA];
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HGU4550023.1"
                     /translation="MLKRIKIVTSLLLVLAVFGLLQLTSGGLFFNALKNDKENFTVLQ
                     TIRQQQSTLNGSWVALLQTRNTLNRAGIRYMMDQNNIGSGSTVAELMQSASISLKQAE
                     KNWADYEALPRDPRQSTAAAAEIKRNYDIYHNALAELIQLLGAGKINEFFDQPTQGYQ
                     DGFEKQYVAYMEQNDRLYDIAVSDNNASYSQAMWILVGVMIVVLAVIFAVWFGIKASL
                     VAPMNRLIDSIRHIAGGDLVKPIEVDGSNEMGQLAESLRHMQGELMRTVGDVRNGANA
                     IYSGASEIATGNNDLSSRTEQQAASLEETAASMEQLTATVKQNAENARQASHLALSAS
                     ETAQRGGKVVDNVVQTMRDISTSSQKIADIISVIDGIAFQTNILALNAAVEAARAGEQ
                     GRGFAVVAGEVRNLAQRSAQAAREIKSLIEDSVGKVDVGSTLVESAGETMAEIVSAVT
                     RVTDIMGEIASASDEQSRGIDQVGLAVAEMDRVTQQNAALVEESAAAAAALEEQASRL
                     TEAVAVFRIQQQQQQQRETSAVVKTVTPAAPRKMAVADSGENWETF"
     gene            complement(2263..3534)
                     /locus_tag="ACHOSC_RS21055"
                     /old_locus_tag="pgaptmp_004211"
     CDS             complement(2263..3534)
                     /locus_tag="ACHOSC_RS21055"
                     /old_locus_tag="pgaptmp_004211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000118634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0031317 - tripartite ATP-independent
                     periplasmic transporter complex [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter large permease"
                     /protein_id="HGU4550024.1"
                     /translation="MSWLLIIPLMIVCLFLNIRVYLAMFIGIIAYFLFFSSVPIEIAV
                     QRLIAPTQSPSLLAIPFFILLGTLMSYTGIAERILHIANVLVGKMRGGLGVANILVST
                     MMGGVSASNLADAAMLSRMMVPEMERKGYNRAFAAAITAGGSLVTPIIPPGIALIIYG
                     LVADVSIGKMFIAGLVPGLLCAVVLMFTVYLVARKTNAKPSRESWPTGKEVVFSLGQA
                     WPALFLIFAVVGGIRANIFTPTEAGAAAVLIVLAIGFFIYREMRISHVVKALGETARA
                     TASVMLVIMASAALGWIFSMEHAGVAVANFVTSLTENKYMFLLIINILLLTLGMFLEG
                     NAILLILVPLLKPVVEHFGIDPVHFGIIMIFNLSIGAFTPPVGTVMLLVCNITQVSVG
                     NFFKQSLPLLAALLLMLTLVTYIPAISLFLV"
     gene            complement(3531..4004)
                     /locus_tag="ACHOSC_RS21060"
                     /old_locus_tag="pgaptmp_004212"
     CDS             complement(3531..4004)
                     /locus_tag="ACHOSC_RS21060"
                     /old_locus_tag="pgaptmp_004212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001235677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter small permease"
                     /protein_id="HGU4550025.1"
                     /translation="MRLFGKVAEFSAAFALFVLVVMTIGAVFMRYFIGQPLQWTEEMS
                     GMLMIWVVMLGGVVAERDRAHLTIPFLMEMLPGKLRRVIAVLVAVLSIALLLYMAWLG
                     YRLAEMAQFKVTQLLKVSWFWIDLAVPVGALATAIYTLYWLIHDIKQSDSGDKMQ"
     gene            complement(4068..5039)
                     /locus_tag="ACHOSC_RS21065"
                     /old_locus_tag="pgaptmp_004213"
     CDS             complement(4068..5039)
                     /locus_tag="ACHOSC_RS21065"
                     /old_locus_tag="pgaptmp_004213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000034588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C4-dicarboxylate TRAP transporter
                     substrate-binding protein"
                     /protein_id="HGU4550026.1"
                     /translation="MSKIIAMLITVSTLFLPSIIQAKPISIKVAYENNPGEPLDVVMR
                     YWADLLNKKSNGEITLALYPSSQLGSKQDVTEQAMMGMNVITLSDVAFLADYEPDLGI
                     LFGPYLTDDPQKLFKIYESDWFKQKNESLKKKGIHVVMNNYLYGTRQIISKKPIRTVD
                     DLAGLKIRVPNNVMQIKAIQAMGATPTPMPLGEVYPALTQGVIDGVENPISVLQGQKL
                     YEQARYLTMVNYLTNTSVWIGGEAFFSTLSPEQLEMIHQTGYEAGVYSQKLTLERDAE
                     MLKTMQAAGVEVIYPDTGPFQKKAREVYSQFPEWTPGLYETIQQQLQ"
BASE COUNT         1356 a         1250 c         1336 g         1237 t
ORIGIN      
        1 ctctaaccat tgcgttgctc caggcgcgag aagcggcgat gagttatttt cgcccaatcg
       61 ttaaacggca taatttgact gagcaacagt ggcgcatcgt gcgtattctg gcggaaagcc
      121 catcaatgga ttttcacgat ctggcgtatc gcgcctgcat tttgcgccca agcctgaccg
      181 ggatcctcac gcggatggaa cgcgacggtt tagtgttgcg attaaagccg atcaatgatc
      241 agcggaagct ctatatctcg ctgaccaaag aggggcaggc gctgtataac cgcgctcaga
      301 cacagattga agaagcctat cggcagattg aagctcagtt tactgccgag aaaatgcaac
      361 agttaaccca tctgttagag gaatttattg ctctgggtaa ttcccgacaa gaagacatcc
      421 cgggcgataa tgaataactt tttagtatct gataaccagc gcctggtttt tccatggatg
      481 gcgggttaca ccttttcata aagtttttgc tttccaggcc gaaaatcttg catcggtcca
      541 caggaaagag aaaccatgtt aaaacgtatc aaaattgtga ccagcttact gctggttttg
      601 gccgtttttg gccttttaca actgacatca ggcggtctgt tctttaatgc cttaaagaat
      661 gacaaagaaa atttcactgt tttacaaacc attcgccagc agcaatccac gctgaatggc
      721 agctgggtcg cgttgttgca gacgcgtaac accctcaacc gcgcgggtat ccgctacatg
      781 atggatcaga ataatattgg tagcggttca accgttgctg agctgatgca gagtgccagt
      841 atttcgctga aacaggcgga aaaaaactgg gcggattacg aagcgttgcc gcgtgacccg
      901 cgtcagagca ccgccgcagc ggcagagatc aaacgtaatt acgatattta tcacaatgcg
      961 ctggcggagc tgatccaact attaggtgca ggcaaaatca acgagttctt tgatcagccg
     1021 acccagggat atcaggacgg tttcgagaag cagtatgtgg cttacatgga gcaaaacgat
     1081 cggctctatg atatcgccgt cagcgataac aatgcctcct acagccaggc gatgtggatt
     1141 ctggtgggcg tgatgatcgt cgtactggcg gtcatcttcg ccgtctggtt cggtattaaa
     1201 gcctcgctgg tagcgccaat gaatcgcctg attgacagca ttcgtcatat tgcaggcggc
     1261 gatctggtga aaccgattga ggtggatggc tctaatgaga tggggcaact ggcagagagt
     1321 ttgcgccata tgcagggaga gctgatgcgt accgtcggtg atgtgcgcaa cggggccaat
     1381 gccatctata gcggtgccag cgaaatcgct accggcaata acgatctctc ttcgcgcacc
     1441 gagcaacagg ccgcttcgct ggaagagacg gcagccagca tggagcaact gaccgcaacg
     1501 gtgaaacaga acgccgagaa tgcgcgccag gccagccatc tggcattaag tgcttctgaa
     1561 acggcgcaac gcggcggcaa agtggtagat aacgtggtgc agactatgcg cgatatctcc
     1621 accagttcgc agaaaatcgc cgatattatc agcgtaattg acggcattgc cttccagacc
     1681 aatattctgg ctttgaacgc ggcggttgag gctgcgcgtg cgggtgagca aggacgcggt
     1741 tttgcggtgg tcgcgggaga agtgcgtaat ctggcccagc gtagcgctca ggcggctcgt
     1801 gaaattaaaa gcctgattga agactcggtg ggaaaagtgg atgttggctc tacgctggtc
     1861 gaaagcgccg gggaaacaat ggcggagatt gtcagtgctg tgacccgcgt gacggacatt
     1921 atgggcgaaa ttgcttctgc ttctgatgag cagagccgtg gtatcgatca ggttggctta
     1981 gcggttgctg agatggaccg ggtaactcaa cagaacgccg cgctggtgga agaatctgcc
     2041 gccgccgccg ccgcgctgga agagcaggcc agtcgcctga ccgaagctgt ggcagtgttc
     2101 cggattcagc aacagcagca acagcagcgt gaaacatcgg ctgtggtaaa aaccgtgacg
     2161 ccagctgcgc cgcgtaaaat ggccgtggca gatagcgggg agaactggga aacgttttaa
     2221 cgcaatgacg ccggatgagt atttcatccg gcgtttttat tattaaacca ggaacagtga
     2281 aatcgccggg atatatgtca ccagcgttag cattaatagt agagccgcca gtagaggcag
     2341 ggattgttta aagaaatttc ccaccgaaac ttgcgtgatg ttacatacca gcagcatcac
     2401 ggtacctacc ggtggcgtga atgcgccaat cgacaggttg aagatcatga tgatgccaaa
     2461 atgcaccgga tcgatgccaa agtgttcgac caccggtttt aatagtggta ccaggatcag
     2521 caaaatggcg tttccttcaa gaaacatccc cagcgtcagc agcaatatgt tgataattaa
     2581 caggaacata tatttatttt cggtcagcga agtcacaaaa ttcgccactg cgacaccagc
     2641 atgttccatt gagaaaatcc atcccaacgc ggcgctggcc atgattacca gcatcaccga
     2701 tgcggttgcg cgtgccgttt cgcccaatgc tttgacgaca tgagaaatcc gcatttcacg
     2761 atagataaaa aagccaatcg ccagcacgat tagcaccgct gctgcacctg cttctgttgg
     2821 ggtgaaaatg ttagcgcgaa taccacccac cacagcaaaa atgaggaaca gcgcaggcca
     2881 ggcctgtccc agcgagaaca ccacctcttt gcccgtaggc cagctttcac gcgagggttt
     2941 agcgttagtt ttgcgggcga ccaggtagac ggtaaacatc agaacgactg cgcatagcaa
     3001 accaggtacc agcccagcga taaacatttt tccgatagag acgtcggcaa ccagaccgta
     3061 aataatcagg gctattcccg gcggtatgat cggggtgact aacgagccac cagcggtaat
     3121 ggcggcggca aacgcgcggt tgtagccttt acgctccatc tccggcacca tcatgcgcga
     3181 cagcatcgcc gcgtcagcaa ggttagaggc gctgacgccg cccatcatgg tgctgacgag
     3241 aatgtttgcc acgccaagtc cgccgcgcat tttccccacc agaacgttgg caatgtgcag
     3301 gatgcgttcg gcaatgccgg tgtagctcat tagtgtgccg agcaagataa aaaacggaat
     3361 tgccagcaat gatgggcttt gcgttggcgc gatcagtctt tgcacggcaa tttcaatcgg
     3421 tacgctggag aagaataaaa agtaagcgat gatgccaata aacatcgcca gatagacccg
     3481 aatgttgaga aacaaacaga cgatcattag cggaataatc aataaccaac tcattgcatt
     3541 ttgtctccgc tatcagattg ttttatatcg tgtattaacc agtagagggt ataaatagcc
     3601 gttgccagtg caccgacagg gaccgccaga tcgatccaga accaggaaac cttcagaagt
     3661 tgtgtcactt taaactgcgc catttcggct agtcggtagc ccagccaggc catatacagc
     3721 agcagtgcga tggagagaac ggcgaccagt actgcaataa cacgcctgag cttgcctgga
     3781 agcatctcca tcaggaaggg aatggtcagg tgcgccctgt cacgttccgc caccactcca
     3841 cctaacatca ccacccaaat catcagcatt cccgacattt cttctgtcca ttgaagcggt
     3901 tggccgatga aataacgcat aaataccgct ccaattgtca tcacaaccag aacgaatagc
     3961 gcaaaggcag cactaaattc tgccactttg ccaaaaagac gcatgaaacc tcctgttagt
     4021 gcgtgtcctg cctaagagag atgaggcagg acgctgagca ttaatgctta ttgcagttgc
     4081 tgttgaatgg tttcgtacag acctggtgtc cattccggga attgagagta aacttcgcgt
     4141 gctttcttct ggaatggtcc agtatcggga tagatcactt cgacgcccgc tgcctgcatg
     4201 gttttcaaca tttcggcatc acgttcaaga gtgagtttct ggctatagac acccgcttca
     4261 tagccggtct ggtggatcat ctccagttgt tctggtgaaa gggtgctaaa gaaggcttca
     4321 cctcctatcc atactgaggt atttgtcagg tagttaacca ttgtcagata tcttgcctgc
     4381 tcatacagtt tttgcccttg cagcactgaa attgggtttt ctacgccgtc aataacgccc
     4441 tgggttaatg cgggataaac ttcgcctaat ggcatgggag ttggtgttgc ccccatcgcc
     4501 tgaatggctt ttatttgcat gacgttatta ggaacacgaa tttttaagcc tgcgagatcg
     4561 tcaacagtgc gaatgggttt tttcgaaata atctgtcgag tgccatagag ataattattc
     4621 attaccacat gaattccctt tttcttcaga ctttcatttt tttgtttaaa ccagtcgctt
     4681 tcatatattt tgaacagttt ttgcggatcg tcagtcagat acggtccaaa caaaataccc
     4741 aaatcgggtt cataatcggc aagaaaggcc acatcactca aggttatgac attcatgccc
     4801 atcatggctt gctcggtgac atcctgtttt gagcccaatt gggaacttgg atagagcgca
     4861 agtgttattt cgccattact ttttttattt aataggtctg cccagtaacg catgaccaca
     4921 tccaggggtt ctccagggtt attttcatag gcaactttta tcgaaatagg ttttgcctgg
     4981 attattgacg gtaaaaaaag ggtactgaca gtaattagca tcgctattat tttgctcatc
     5041 tttacttact ccatgctaag gaaataaaac tccaacattg ttgtcgggag tgaaacctta
     5101 tgaaaaagca gtcattactg aaaagtagtc agcgaatagc ggaatggctg caaatgtgtc
     5161 ataaattcgt gcaccgggt
//
LOCUS       DAZKHF020000281         5170 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2201S31241
            SAMN43267800-rid25106413.denovo.283, whole genome shotgun sequence.
ACCESSION   DAZKHF020000281 DAZKHF020000000
VERSION     DAZKHF020000281.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267800
            Sequence Read Archive: SRR30307263
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5170)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5170)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 5170)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350581.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350581.2
            Genome Coverage       :: 151x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:21:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,652
            CDSs (total)                      :: 5,557
            Genes (coding)                    :: 5,320
            CDSs (with protein)               :: 5,320
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 237
            CDSs (without protein)            :: 237
            Pseudo Genes (ambiguous residues) :: 0 of 237
            Pseudo Genes (frameshifted)       :: 102 of 237
            Pseudo Genes (incomplete)         :: 130 of 237
            Pseudo Genes (internal stop)      :: 52 of 237
            Pseudo Genes (multiple problems)  :: 41 of 237
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5170
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267800-rid25106413.denovo.283"
                     /serotype="O 26:H11"
                     /isolate="2201S31241"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-01"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            <1..734
                     /locus_tag="ACHOSC_RS21070"
                     /old_locus_tag="pgaptmp_004214"
     CDS             <1..734
                     /locus_tag="ACHOSC_RS21070"
                     /old_locus_tag="pgaptmp_004214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="fimbrial chaperone"
                     /protein_id="HGU4550027.1"
                     /translation="NTKHTTALCFVTCMAFSSSSIADIVISGTRVIYKSDQKSVNVRL
                     ENKGNNPLLVQSWLDTGDDNAEPGSITVPFTATPPVSRIDAKRGQTIKLMYTASTSLP
                     KDRESVFWFNVLEVPPKPDAEKVANQSLLQLAFRTRIKLFYRPDGLKGNPSEAPLALK
                     WFWSGSEGKASLRVTNPTPYYVSFSSGDLEASGKRYPIDVKMIAPFSDEVMKVTGLNG
                     KASSAKVHFYAINDFGGAIEGNASL"
     gene            769..3369
                     /gene="htrE"
                     /locus_tag="ACHOSC_RS21075"
                     /old_locus_tag="pgaptmp_004215"
     CDS             769..3369
                     /gene="htrE"
                     /locus_tag="ACHOSC_RS21075"
                     /old_locus_tag="pgaptmp_004215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Yad fimbria usher protein HtrE"
                     /protein_id="HGU4550028.1"
                     /translation="MTIKPTKNYHNHLTRIATFCALLYCNSAFCAELVEYDHTFLMGQ
                     NASNIDLSRYSEGNPAIPGMYDVSVYVNDQPIINQSITFIEIEGKKNAQACITLKNLL
                     QFHINSPDINNEKAVLLARDETLGNCLNLTEIIPQASVRYDVNEQRLDIDVPQAWVMK
                     NYQNYVDPSLWENGINAAMLSYNLNGYHSETPGRRNDSIYAAFNGGMNLGAWRLRASG
                     NYNWMTDSGSNYDFKNRYIQRDIASLRSQLILGESYTTGETFDSVSIRGIRLYSDSRM
                     LPPTLASFAPIIHGVANTNAKVTITQGGYKIYETTVPPGAFVIDDLSPSGYGSDLIVT
                     VEESDGSKRTFSQPFSSVVQMLRPGVGRWDISGGQVLKDDIQDEPNLFQASYYYGLNN
                     YLTGYTGIQITDNNYTAGLLGLGLNTSVGAFSFDVTHSNVRIPDDKTYQGQSYRVSWN
                     KLFEETSTSLNIAAYRYSTQNYLGLNDALTLIDEVKHPEQDLEPKSMRNYSRMKNQVT
                     VSINQPLKFEKKDYGSFYLSGSWSDYWASGQNRSNYSIGYSNSASWGSYSVSAQRSWN
                     EDGDTDDSVYLSFTIPIEKLLGTEQRTSGFQSIDTQMSSDFKGNNQLNVSSSGYSDNA
                     RVSYSVNTGYTMNKASKDLSYVGGYASYESPWGTLAGSVSANSDNSRQVSLSTDGGFV
                     LHSGGLTFSNDSFSDSDTLAVVQAPGAQGARINYGNSTIDRWGYGVTSALSPYHENRI
                     ALDINDLENDVELKSTSAVAVPRQGSVVFADFETVQGQSAIMNITRSDGKNIPFAADI
                     YDEQGNVIGNVGQGGQAFVRGIEQQGNISIKWLEESKPVSCLAHYQQSSEAEKIAQSI
                     ILNGIRCQIQ"
     gene            3386..3955
                     /locus_tag="ACHOSC_RS21080"
                     /old_locus_tag="pgaptmp_004216"
     CDS             3386..3955
                     /locus_tag="ACHOSC_RS21080"
                     /old_locus_tag="pgaptmp_004216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414680.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HGU4550029.1"
                     /translation="MIKITPHKIAILMGILLSPSVFATDINVEFTATVKATTCNITLT
                     GNNVTNDGNNNYTLRIPKMGLDKIANKTTESQADFKLVASGCSSGISWIDTTLSGNAS
                     SSSPKLIIPQSGDSSSTTSNIGMGFKKRTTDDATFLKPNSAEKIRWSTDEMQPDKGLE
                     MTVALRETDAGQGVPGNFRALATFNFIYQ"
     gene            3970..4572
                     /gene="yadL"
                     /locus_tag="ACHOSC_RS21085"
                     /old_locus_tag="pgaptmp_004217"
     CDS             3970..4572
                     /gene="yadL"
                     /locus_tag="ACHOSC_RS21085"
                     /old_locus_tag="pgaptmp_004217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000143198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial-like protein YadL"
                     /protein_id="HGU4550030.1"
                     /translation="MTFKNLRYGLSSSVVLAASLFSVPSYAATDSIGLTVITTVEIGT
                     CTATLVNDSDQDISVVDFGDVYISEINAKTKVKTFKLKFKDCAGIPNKKAQIKLTKRA
                     TCEGTANDGAGFANGSTAADKASAVAVEVWSTETPATGSATQFSCVTPASQEVTISNA
                     ANAVVYYPMSARLVVEKNKTVSNVTAGKFSAPATFTVTYN"
     gene            4599..>5170
                     /locus_tag="ACHOSC_RS21090"
                     /old_locus_tag="pgaptmp_004218"
     CDS             4599..>5170
                     /locus_tag="ACHOSC_RS21090"
                     /old_locus_tag="pgaptmp_004218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial-like protein"
                     /protein_id="HGU4550031.1"
                     /translation="MHPTQRKLMKRIILFLSLQFPITYPAIAGQDIDLVANVKNSTCK
                     SGISNQGNIDLGVVGVGYFSDNVTPESYQPGGKEFTVTVSDCALQGTGDVLNQLHIDF
                     RALSGVMAAGSRQIFANEVATGAKNVGVVLFSIQDPTNIFNVISSAGNSRSVYPVMTS
                     ALHNSSWKFYARMQKIDPALDVISGQVMSH"
BASE COUNT         1528 a         1086 c         1149 g         1407 t
ORIGIN      
        1 ttaacaccaa acatacaaca gctttatgct tcgtaacctg tatggctttt agttcatcgt
       61 ctattgcgga cattgtcatt tcgggtactc gcgtaatata taaaagcgat caaaaaagtg
      121 tcaacgtacg tctggaaaat aaagggaata acccgttgct tgtccagagt tggttagata
      181 ctggcgatga caacgctgag cctggcagta ttacagtccc ttttactgcc acgccgccag
      241 tatcgcgtat tgatgccaaa cgtgggcaaa caatcaaatt aatgtacaca gccagcacct
      301 cactgcctaa agacagagag agcgtgttct ggtttaacgt actggaagtt ccaccaaaac
      361 cagatgcaga aaaggtcgcg aatcaaagtc tgctgcaact ggcatttcgc acacgtataa
      421 aacttttcta tcgcccggat ggattgaagg gaaatccctc tgaagccccg ttagccctga
      481 agtggttctg gtcaggttca gaaggtaagg cgtcattgcg agtgaccaat ccaacccctt
      541 actacgtctc ttttagcagt ggtgatttag aagctagcgg taaacgctat ccgattgatg
      601 tgaaaatgat tgcaccattt agtgatgagg tcatgaaagt cactggcctt aatggcaaag
      661 caagttctgc aaaagtacat ttttacgcca ttaatgattt tggtggcgcc attgaaggta
      721 atgccagtct gtaatcaggc aaggatataa ttccgcagga agcataacgt gactataaaa
      781 ccaactaaaa attatcataa tcatctgacc cgtatcgcta cgttttgcgc gctgctgtat
      841 tgcaatagtg ctttctgtgc tgaacttgtt gaatatgacc ataccttcct gatggggcag
      901 aatgcctcaa atattgatct cagccggtat agtgaaggta accctgctat accgggtatg
      961 tacgacgtca gtgtttatgt caacgatcaa cctattatta accaaagcat tacatttatt
     1021 gaaattgaag gaaaaaaaaa cgcacaagcc tgtatcacac ttaagaattt attgcagttt
     1081 catataaatt ctcccgatat aaataacgaa aaagccgttc tgcttgccag ggatgaaaca
     1141 ctcggcaatt gccttaattt gacggaaatt atccctcagg cttctgttcg ttatgacgtt
     1201 aacgagcaac gtctggatat agacgttcct caggcatggg taatgaaaaa ttaccaaaac
     1261 tatgttgatc catcgttatg ggaaaatggc attaatgcgg ccatgttgtc atacaacctc
     1321 aacggatatc atagtgaaac tcctggtcga agaaatgaca gcatttatgc tgcttttaac
     1381 ggtgggatga atttaggtgc atggcgactg cgtgcctcag gcaactacaa ctggatgacc
     1441 gattctggca gtaattatga ttttaagaat cggtatatac agcgtgatat cgcctcgctg
     1501 cgttctcaac tcattcttgg tgagtcttat acaacgggcg aaacctttga ttctgtcagt
     1561 atccgcggca ttcgtttata cagcgacagc cgcatgttgc ctccgacatt agcgagtttt
     1621 gcgcctatca ttcatggcgt tgctaatacc aacgccaaag tcactattac gcaaggtggc
     1681 tataagattt atgaaacgac tgtgccgcca ggcgctttcg tcattgatga tctgagtccg
     1741 tcaggttacg gcagcgatct tattgttact gtcgaagaat ccgatggctc aaagcggaca
     1801 ttctcgcaac ctttttcatc ggtcgttcaa atgttacgcc ctggcgttgg acgttgggat
     1861 attagcggcg gccaggtctt aaaagatgat attcaggatg agcctaattt atttcaggcc
     1921 agctactact acggtctgaa taactatcta acgggttata ctggtattca gattaccgat
     1981 aataactata ccgctggttt gttaggtctt ggtctgaata cttcagtcgg tgcattttct
     2041 ttcgatgtga ctcactccaa tgttcgtata ccggatgata aaacatatca gggacaaagt
     2101 tatcgtgttt cctggaacaa gttattcgaa gaaacaagta cttcactgaa tatcgcggcc
     2161 tatcgctatt cgacacagaa ttaccttggt cttaatgatg cactaactct aattgatgaa
     2221 gtgaaacatc ccgaacaaga tcttgaaccg aaatccatgc gtaattactc gcgcatgaaa
     2281 aatcaggtta cggtcagtat taaccaaccg ctaaaatttg agaaaaaaga ttacggttca
     2341 ttttatcttt ccggaagttg gtccgattac tgggcttccg gacaaaatcg tagcaattac
     2401 tctattggct acagtaacag tgcatcctgg ggcagctaca gtgtcagtgc ccagcgttca
     2461 tggaatgaag acggcgacac tgacgatagc gtttatctca gtttcaccat tcctattgaa
     2521 aaattacttg gcactgaaca acggacttca ggtttccaga gtattgatac tcaaatgagc
     2581 agtgacttta agggtaataa ccaactcaac gtcagtagca gtggctatag tgataacgct
     2641 cgcgtcagtt atagcgtaaa tactggctat acgatgaata aagccagcaa agatttgagt
     2701 tatgttgggg gttatgccag ctatgaatca ccgtggggaa ccctggcagg ttcagtttct
     2761 gcaaatagcg ataacagtcg tcaagtgtct ctcagcaccg acggtggttt tgtattgcat
     2821 agcggcggcc tgaccttcag taatgacagt tttagcgact ccgatacact ggcggtagtt
     2881 caggctccag gtgctcaagg agcgcgaata aattatggca acagtactat cgatcgatgg
     2941 ggttatggtg tcaccagcgc tctttctcct tatcatgaaa accgtatcgc gctggatatc
     3001 aacgatcttg agaacgatgt tgaattaaaa agtaccagtg cagtagctgt accgcgtcag
     3061 ggttcagtcg tctttgctga ttttgaaacc gtgcaagggc aatcagccat tatgaacatc
     3121 acacgaagtg atggtaaaaa tattccattt gctgcagata tttatgatga gcaaggcaat
     3181 gtcattggta atgttggaca gggtggacaa gcatttgttc gtggtattga gcagcaggga
     3241 aatatcagca ttaaatggct cgaagaaagt aaacccgtaa gttgtcttgc gcattatcaa
     3301 caaagttcag aagcagaaaa aatagcgcaa tctattattc tgaatggaat tcggtgtcag
     3361 attcagtaac tacaagggac gtcaaatgat aaaaataacg ccacataaaa tagcgatact
     3421 gatgggaata ttattatcac catcagtatt tgcaacggat attaatgtag agtttacagc
     3481 cactgtcaaa gcgacaacct gtaacatcac acttactggt aataacgtca cgaatgatgg
     3541 caataataac tacacattga gaatccctaa gatgggtctg gataagatcg cgaataaaac
     3601 gacagaatct caggctgatt ttaaactggt tgctagtggg tgcagcagtg gcatcagttg
     3661 gattgatacc actctgagcg gaaatgcatc atcaagctca cctaagctta ttataccgca
     3721 gtctggtgat tcatcttcga cgacaagtaa tatcggtatg ggtttcaaaa aacggactac
     3781 tgatgatgcc actttcctta aacctaacag tgcggaaaag atacgctgga gcacagacga
     3841 gatgcagccc gataagggtc ttgaaatgac cgttgcgcta cgtgaaacag atgcagggca
     3901 aggcgtaccg gggaattttc gtgcactggc aacgtttaat ttcatctatc aataagaagg
     3961 ataattaaga tgacatttaa aaatttacgt tatggattat ccagcagtgt cgttttggct
     4021 gcctcattgt tcagcgtacc ctcttatgcg gcaacagaca gcattgggct gaccgtgatt
     4081 actaccgtag aaataggtac ttgtaccgct acattagtaa atgactctga tcaggacatt
     4141 tctgttgttg actttggcga tgtatatatt tctgaaatca atgccaagac caaagttaaa
     4201 acattcaaac tcaaattcaa agactgtgcg ggtatcccca ataaaaaagc acaaataaaa
     4261 ttaaccaagc gagccacatg cgaaggtact gctaatgacg gtgcggggtt cgcaaatgga
     4321 tccacagccg cagataaagc aagtgccgtc gccgttgaag tctggagcac cgaaactccg
     4381 gcaacaggga gcgcaacaca atttagttgt gtgacaccag catctcaaga ggtcactatc
     4441 tccaatgcag ccaacgcggt tgtttattat ccgatgagtg ctcgactggt cgtcgaaaaa
     4501 aataaaaccg taagcaatgt cactgcgggt aaattttctg cacctgccac atttacagta
     4561 acctataact aacagactga cagatccagg aactatatat gcatcccact caacgtaagc
     4621 tgatgaagag aataattctg tttctgtcac tacagtttcc cattacctat ccagccatcg
     4681 ctgggcagga tattgacctt gttgccaatg taaaaaacag cacttgcaaa agcggaatca
     4741 gtaaccaggg taatattgat cttggcgtcg ttggggtggg atatttttca gataatgtta
     4801 ctcctgaaag ctatcaacca ggtggaaaag agttcactgt cacagtatca gactgtgcat
     4861 tacagggaac tggcgatgtg cttaatcagt tacatattga ttttagagcc cttagcggtg
     4921 tcatggctgc aggttcaagg caaatatttg ccaatgaagt cgcaaccggg gctaaaaatg
     4981 tcggtgtcgt tctcttttcc attcaggacc cgaccaatat attcaatgtt attagttcag
     5041 caggaaattc tcgttccgtg tatccagtaa tgacttctgc cctgcataat tcttcatgga
     5101 aattttatgc cagaatgcaa aaaatcgatc ccgcgctgga tgtcatatct ggccaggtaa
     5161 tgagtcatat
//
LOCUS       DAZKHF020000282         5123 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2201S31241
            SAMN43267800-rid25106413.denovo.284, whole genome shotgun sequence.
ACCESSION   DAZKHF020000282 DAZKHF020000000
VERSION     DAZKHF020000282.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267800
            Sequence Read Archive: SRR30307263
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5123)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5123)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 5123)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350581.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350581.2
            Genome Coverage       :: 151x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:21:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,652
            CDSs (total)                      :: 5,557
            Genes (coding)                    :: 5,320
            CDSs (with protein)               :: 5,320
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 237
            CDSs (without protein)            :: 237
            Pseudo Genes (ambiguous residues) :: 0 of 237
            Pseudo Genes (frameshifted)       :: 102 of 237
            Pseudo Genes (incomplete)         :: 130 of 237
            Pseudo Genes (internal stop)      :: 52 of 237
            Pseudo Genes (multiple problems)  :: 41 of 237
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5123
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267800-rid25106413.denovo.284"
                     /serotype="O 26:H11"
                     /isolate="2201S31241"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-01"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            <1..834
                     /locus_tag="ACHOSC_RS21095"
                     /old_locus_tag="pgaptmp_004219"
     CDS             <1..834
                     /locus_tag="ACHOSC_RS21095"
                     /old_locus_tag="pgaptmp_004219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="major capsid protein"
                     /protein_id="HGU4550032.1"
                     /translation="SRGGSTSEFTPGYVKPKHEVNPQMTLRRLPDEDPQNLADPAYRR
                     RRIILQNMRDEELAIAQVEEMQAVSAVLKGKYTMTGEAFDPVEVDMGRSVANNITQSG
                     GTEWSKRDKSTYDPTDDIEAYALNASGVVNIIVFDPKGWALFRSFKAVKEKLDTRRGS
                     HSELETAVKDLGEAVSYKGMYGDTAIVVYSGQYVENDVKKNFLPDNTMVLGNTQARGL
                     RTYGCIQDADAQREGINASARYPKNWVTTGDPAREFTMIQSAPLMLLADPDAFVSVQL
                     A"
     gene            876..1271
                     /locus_tag="ACHOSC_RS21100"
                     /old_locus_tag="pgaptmp_004220"
     CDS             876..1271
                     /locus_tag="ACHOSC_RS21100"
                     /old_locus_tag="pgaptmp_004220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-packaging protein FI"
                     /protein_id="HGU4550033.1"
                     /translation="MTKDELIARLQVLGEQLNRDVSLTGTKEELALRVAELEEELDDT
                     DDAAGQDTSVSPENALTGHENEVVSAQPDTVTDTADLVTVVALVTLHTDALHATRDEA
                     VAFVLPGTAFRVSAGVAAEMTERGLARMQ"
     gene            1283..1657
                     /locus_tag="ACHOSC_RS21105"
                     /old_locus_tag="pgaptmp_004221"
     CDS             1283..1657
                     /locus_tag="ACHOSC_RS21105"
                     /old_locus_tag="pgaptmp_004221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000752961.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="head-tail joining protein"
                     /protein_id="HGU4550034.1"
                     /translation="MADFDNLFDAAIACADETIRGYMGTSATMTSGEQSGAVIRGVFD
                     DPENISYAGQGVRVEGSSPSLFVRTDDVRQLRRGDTLTIGEENFWIDRISPDDGGSCH
                     LWLGRGVPPAVNRRRWKGDVWP"
     gene            1648..2226
                     /locus_tag="ACHOSC_RS21110"
                     /old_locus_tag="pgaptmp_004222"
     CDS             1648..2226
                     /locus_tag="ACHOSC_RS21110"
                     /old_locus_tag="pgaptmp_004222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HGU4550035.1"
                     /translation="MAIKGLEQAVENLSRISRTAVPGAAAMAINRVASSAISQSVSQV
                     ARETKVRRKLVKERARLKRATVKNPQARIKVNRGDLPVIKLGNARVVLSRRRRRKKGQ
                     RSSLKGGGSVLVVGNRRIPGAFIQQLKNGRWHVMQRVAGKNRYPIDVVKIPMAVPLTT
                     AFKQNIERIRRERLPKELGYALQHQLRMVIKR"
     gene            2223..2618
                     /locus_tag="ACHOSC_RS21115"
                     /old_locus_tag="pgaptmp_004223"
     CDS             2223..2618
                     /locus_tag="ACHOSC_RS21115"
                     /old_locus_tag="pgaptmp_004223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage minor tail U family protein"
                     /protein_id="HGU4550036.1"
                     /translation="MKHTELRAAVLDALEKHDTGATFFDGRPAVFDEADFPAVAVYLT
                     GAEYTGEELDSDTWQAELHIEVFLPAQVPDSELDAWMESRIYPVMSDIPALSELITSM
                     VASGYDYRRDDDAGLWSSADLTYVITYEM"
     gene            2626..3366
                     /locus_tag="ACHOSC_RS21120"
                     /old_locus_tag="pgaptmp_004224"
     CDS             2626..3366
                     /locus_tag="ACHOSC_RS21120"
                     /old_locus_tag="pgaptmp_004224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HGU4550037.1"
                     /translation="MPVPNPVMPVKGAGTTLWVYKGSGDPYANPLSDVDWSRLAKVKD
                     LTPGELTAESYDDSYLDDEDADWTATGQGQKSAGDTSFTLAWMPGEQGQQALLAWFNE
                     GDTRAYKIRFPNGTVDVFRGWVSSIGKAVTAKEVITRTVKVTNVGRPSMAEDRSTVTA
                     ATGMTVTPASTSVVKGQSTTLTVAFQPEGATDKSFRAVSADKTKATVSVSGMTITVKG
                     VAAGKVNIPVVSGNGELAAVAEITVTDS"
     gene            3382..3804
                     /locus_tag="ACHOSC_RS21125"
                     /old_locus_tag="pgaptmp_004225"
     CDS             3382..3804
                     /locus_tag="ACHOSC_RS21125"
                     /old_locus_tag="pgaptmp_004225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage minor tail protein G"
                     /protein_id="HGU4550038.1"
                     /translation="MFLKTESFEHNGVTVTLSELSALQRIEHLALMKRQAEQAESDSN
                     RKFTVEDAIRTGAFVVAMSLWHNHPQKTKQPSMNEAVKQIEQEVLTTWPTEAISHAEN
                     VVYRLSGMYEFVVNDAPEQAEDAGPAEPVSAGKCSTVS"
     gene            3786..4220
                     /locus_tag="ACHOSC_RS21130"
                     /old_locus_tag="pgaptmp_004226"
     CDS             3786..4220
                     /locus_tag="ACHOSC_RS21130"
                     /old_locus_tag="pgaptmp_004226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail assembly protein T"
                     /protein_id="HGU4550039.1"
                     /translation="MFDGELSFALKLAREMGRPDWRAMLAGMSSTEYADWHRFYSTHY
                     FHDVLLDMHFSGLTYTVLSLFFSDPDMHPLDFSLLNRREADEEPEDDVLMQKAAGLAG
                     GVRFGPDGNEVIPASPDVADMTEDDVMLMTVSEGIAGGVRYG"
     gene            4213..>5123
                     /locus_tag="ACHOSC_RS21135"
                     /old_locus_tag="pgaptmp_004227"
     CDS             4213..>5123
                     /locus_tag="ACHOSC_RS21135"
                     /old_locus_tag="pgaptmp_004227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail length tape measure family protein"
                     /protein_id="HGU4550040.1"
                     /translation="MAEPVGDLVVDLSLDAARFDEQMARVRRHFSGTESDAKKTAAVV
                     EQSLSRQALAAQKAGISVGQYKAAMRMLPAQFTDVATQLAGGQSPWLILLQQGGQVKD
                     SFGGMIPMFRGLAGAITLPMVGATSLAVATGALAYAWYQGNSTLSDFNKTLVLSGNQA
                     GLTADRMLVLSRAGQAAGLTFNQTSESLSALVKAGVSGEAQIASISQSVARFSSASGV
                     EVDKVAEAFGKLTTDPTSGLTAMARQFHNVTAEQIAYVAQLQRSGDEAGALQAANEAA
                     TKGFDDQTRRLKENMGTLETWADRTARA"
BASE COUNT         1133 a         1313 c         1642 g         1035 t
ORIGIN      
        1 tcccgtggcg gctccacctc tgaatttacg ccgggatatg tcaaacccaa gcatgaagtg
       61 aatccgcaga tgaccctgcg tcgcctgccg gatgaagatc cacagaatct ggcggacccg
      121 gcttaccgcc gccgtcgcat catcctgcag aacatgcgag acgaagagct ggccattgct
      181 caggtcgaag agatgcaggc agtttctgcc gtgcttaagg gcaaatacac catgaccggt
      241 gaagccttcg atccggttga agtggatatg ggccgcagtg tggcgaacaa catcacgcag
      301 tccggcggta cggagtggag caagcgtgac aagtccacgt atgacccgac cgacgatatc
      361 gaagcctacg cgctgaacgc cagcggtgtg gtgaatatca tcgtgtttga tccgaaaggc
      421 tgggcgctgt tccgttcctt caaagccgtc aaggagaagc tggatacccg tcgcggctct
      481 cattccgagc tggagacagc ggtaaaagac ctgggcgaag cggtgtccta taaggggatg
      541 tatggcgata cggcgatcgt cgtgtattcc ggacagtacg tggaaaacga cgtcaaaaag
      601 aacttccttc cggacaacac gatggtgctg gggaacactc aggcacgcgg tctgcgcacc
      661 tatggctgca ttcaggatgc ggacgcacag cgcgaaggta ttaacgcctc tgcccgctac
      721 ccgaaaaact gggtgaccac cggcgatccg gcgcgtgagt tcaccatgat tcagtcagca
      781 ccgctgatgc tgctggctga ccctgatgcg ttcgtgtccg tacaactggc gtaatcatgg
      841 cccttcgggg ccattttctc tctgtggagg agtccatgac gaaagatgaa ctgattgccc
      901 gtcttcaggt gctgggtgag caactgaacc gtgatgtcag cctgacgggg acgaaagaag
      961 aactggcact ccgtgtggca gagctggaag aggagcttga tgacacggat gacgctgccg
     1021 gtcaggacac atctgtcagc ccggaaaatg cgctgaccgg acatgaaaat gaggttgtat
     1081 cagcgcagcc ggataccgtg actgatacgg ctgatctggt cacggttgtg gcactggtga
     1141 cgctgcatac tgatgcactt cacgccacgc gggatgaggc tgtggcattt gtgctgccgg
     1201 gaacggcgtt ccgtgtctct gccggtgtgg cagctgaaat gacagagcgc ggcctggcca
     1261 gaatgcaata acgggaggcg ctgtggctga tttcgataac ctgttcgatg ctgccattgc
     1321 ctgcgccgat gaaacgatac gcgggtacat gggaacgtca gccaccatga catccggtga
     1381 gcagtccggt gctgtgatac gtggtgtttt tgatgaccct gaaaatatca gctatgccgg
     1441 acagggcgtg cgcgttgaag gctccagccc gtccctgttt gtccggactg atgatgtgcg
     1501 gcagctgcgg cgcggcgaca cgctgaccat cggtgaggaa aacttctgga tagaccggat
     1561 ttcgccggat gatggcggaa gctgtcatct ctggcttggg cggggcgtac cgcctgccgt
     1621 taaccgtcgc cgctggaagg gggatgtatg gccataaaag gtcttgagca ggccgttgaa
     1681 aacctcagcc gtatcagcag aacggcggtg cccggtgccg ccgcaatggc cattaaccgc
     1741 gttgcttcat ccgcgatatc gcagtcggtg tcacaggttg cccgtgagac aaaggtacgc
     1801 cggaaactgg taaaggaaag ggccaggctg aaaagggcca cggtcaaaaa tccgcaggcc
     1861 agaatcaagg ttaaccgggg ggatttgccc gtaatcaagc tgggtaatgc gcgggttgtc
     1921 ctttcgcgcc gcaggcgtcg taaaaagggg cagcgttcat ccctgaaagg tggcggcagc
     1981 gtgcttgtgg tgggtaaccg tcgtattccc ggcgcgttta ttcagcaact gaaaaatggc
     2041 cggtggcatg ttatgcagcg tgtggccggg aaaaaccgtt accccattga tgtggtgaaa
     2101 atcccgatgg cggtgccgct gaccacggcg tttaaacaaa atattgagcg gatacggcgt
     2161 gaacgtcttc cgaaagagct gggctatgcg ctgcagcatc aactgaggat ggtaataaag
     2221 cgatgaaaca tactgaactc cgtgcagccg tactggatgc actggagaag catgacaccg
     2281 gggcgacgtt ttttgatggt cgccccgctg tttttgatga ggcggatttt ccggcagttg
     2341 ccgtttatct caccggcgct gaatacacgg gcgaagagct ggacagcgat acctggcagg
     2401 cggagctgca tatcgaagtt ttcctgcctg ctcaggtgcc ggattcagag ctggatgcgt
     2461 ggatggagtc ccggatttat ccggtgatga gcgatatccc ggcactgtca gagttgatca
     2521 ccagtatggt ggccagtggc tatgactacc gacgcgacga tgatgcgggc ctgtggagtt
     2581 cagccgatct gacttatgtc attacctatg aaatgtgagg acgctatgcc tgtaccaaat
     2641 cctgtaatgc cggtgaaagg tgccgggacc accctgtggg tttataaggg gagcggtgac
     2701 ccttatgcga atccgctttc agacgttgac tggtcgcgtc tggcaaaagt taaagacctg
     2761 acgcccggcg aactgaccgc tgagtcctat gacgacagct atctcgatga tgaagatgcg
     2821 gactggactg cgaccgggca ggggcagaaa tcagccggag ataccagctt cacgctggcg
     2881 tggatgcccg gagagcaggg gcagcaggcg ctgctggcgt ggtttaatga aggtgatacc
     2941 cgtgcctata aaatccgctt cccgaacggc acggtcgatg tgttccgtgg ctgggtcagc
     3001 agtatcggta aagcggtgac ggcgaaggaa gtgatcaccc gcacggtgaa ggtcaccaat
     3061 gtgggacgcc cgtcgatggc agaagatcgc agcacggtca cagcggcaac cggcatgacc
     3121 gtgacgcctg ccagcacctc ggtggtgaaa gggcagagca ccacgctgac cgtggcattc
     3181 cagccggaag gcgcaaccga caagagcttc cgtgcggtgt ctgcggataa aacaaaagcc
     3241 accgtgtcgg tcagtggtat gaccatcacc gtgaaaggtg ttgctgcagg caaggtcaac
     3301 attccggtcg tatccggtaa tggtgaactt gctgcggttg cagaaatcac tgtcaccgac
     3361 agttaatccg gagagtcagc gatgttcctg aaaaccgaat catttgaaca taacggcgtg
     3421 accgtcacgc tttctgaact ttcagccctg cagcgtattg agcatctcgc cctgatgaaa
     3481 cggcaggcag aacaggcgga gtcagacagc aaccggaagt ttacagtgga agacgccatc
     3541 agaaccggtg cttttgtggt ggcgatgtcc ctgtggcata accatccgca gaagacaaag
     3601 cagccttcca tgaatgaagc cgttaaacag attgagcagg aagtgcttac cacctggccc
     3661 acagaggcaa tttctcatgc tgaaaacgtg gtgtaccggc tgtccggtat gtatgagttt
     3721 gtggtgaatg atgcccctga acaggcagag gacgccgggc ctgcagagcc tgtttctgcg
     3781 ggaaagtgtt cgacggtgag ctgagttttg ccctgaaact ggcgcgcgag atggggcgac
     3841 ccgactggcg tgccatgctt gccgggatgt catccacgga gtatgccgac tggcaccgct
     3901 tttacagtac ccattatttt catgatgttc tgctggatat gcacttttcc gggctgacgt
     3961 acaccgtgct cagcctgttt ttcagcgatc cggatatgca tccgctggat ttcagtctgc
     4021 tgaaccggcg tgaggctgac gaagagcctg aagatgatgt gctgatgcag aaagcggcag
     4081 ggcttgccgg aggcgtccgc tttggcccgg acgggaatga agttatcccc gcttccccgg
     4141 atgtggcgga catgacggag gatgacgtaa tgctgatgac agtatcagaa gggatcgcag
     4201 gaggagtccg gtatggctga accggtaggc gatctggtcg ttgatttaag tctggatgcg
     4261 gccagatttg acgagcagat ggccagagtc aggcgtcatt tttccggtac ggaaagtgat
     4321 gcgaaaaaaa cagcggcagt cgttgaacag tcgctgagcc gacaggcgct ggctgcacag
     4381 aaagcgggga tttccgtcgg gcagtataaa gccgccatgc gtatgctgcc tgcacagttc
     4441 accgacgtgg ccacgcagct tgcaggcggg caaagtccgt ggctgatcct gctgcaacag
     4501 ggggggcagg tgaaggactc cttcggcggg atgatcccca tgttcagggg gcttgccggt
     4561 gcgatcaccc tgccgatggt gggggccacc tcgctggcgg tggcgaccgg tgcgctggcg
     4621 tatgcctggt atcagggcaa ctcaaccctg tccgatttca acaaaacgct ggtcctttcc
     4681 ggcaatcagg cgggactgac ggcagatcgt atgctggtcc tgtccagagc cgggcaggcg
     4741 gcagggctga cgtttaacca gaccagcgag tcactcagcg cactggttaa ggcgggggta
     4801 agcggtgagg ctcagattgc gtccatcagc cagagtgtgg cgcgtttctc ctctgcatcc
     4861 ggcgtggagg tggacaaggt cgctgaagcc ttcgggaagc tgaccacaga cccgacgtcg
     4921 gggctgacgg cgatggcacg ccagttccat aacgtgacgg cggagcagat tgcgtatgtt
     4981 gctcagttgc agcgttccgg cgatgaagcc ggggcattgc aggcggcgaa cgaggccgca
     5041 acgaaagggt ttgatgacca gacccgccgc ctgaaagaga acatgggcac gctggagacc
     5101 tgggcagaca ggactgcgcg ggc
//
LOCUS       DAZKHF020000283         5070 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2201S31241
            SAMN43267800-rid25106413.denovo.285, whole genome shotgun sequence.
ACCESSION   DAZKHF020000283 DAZKHF020000000
VERSION     DAZKHF020000283.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267800
            Sequence Read Archive: SRR30307263
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5070)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5070)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 5070)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350581.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350581.2
            Genome Coverage       :: 151x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:21:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,652
            CDSs (total)                      :: 5,557
            Genes (coding)                    :: 5,320
            CDSs (with protein)               :: 5,320
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 237
            CDSs (without protein)            :: 237
            Pseudo Genes (ambiguous residues) :: 0 of 237
            Pseudo Genes (frameshifted)       :: 102 of 237
            Pseudo Genes (incomplete)         :: 130 of 237
            Pseudo Genes (internal stop)      :: 52 of 237
            Pseudo Genes (multiple problems)  :: 41 of 237
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5070
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267800-rid25106413.denovo.285"
                     /serotype="O 26:H11"
                     /isolate="2201S31241"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-01"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            complement(<1..939)
                     /locus_tag="ACHOSC_RS21140"
                     /old_locus_tag="pgaptmp_004228"
     CDS             complement(<1..939)
                     /locus_tag="ACHOSC_RS21140"
                     /old_locus_tag="pgaptmp_004228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6531 domain-containing protein"
                     /protein_id="HGU4550041.1"
                     /translation="MSGKPAARQGDMTQYGGSIVQGSAGVRIGAPTGVACSVCPGGVT
                     SGHPVNPLLGAKVLPGETDIALPGPLPFILSRTYSSYRTKTPAPVGSLGPGWKMPADI
                     RLQLRDNTLILSDNGGRSLYFEHLFPGEDGYSRSESLWLVRGGVAKLDEGHRLAALWQ
                     ALPEELRLSPHRYLATNSPQGPWWLLGWCERVPEADEVLPAPLPPYRVLTGLVDRFGR
                     TQTFHREAAGEFSGEITGVTDGAGRHFRLVLTTQAQRAEEARQQAISGGTEPSAFPDT
                     LPGYTEYGRDNGIRLSAVWLTHDPEYPENLPAAPLVR"
     gene            1168..1776
                     /locus_tag="ACHOSC_RS21145"
                     /old_locus_tag="pgaptmp_004229"
     CDS             1168..1776
                     /locus_tag="ACHOSC_RS21145"
                     /old_locus_tag="pgaptmp_004229"
                     /EC_number="2.5.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase"
                     /protein_id="HGU4550042.1"
                     /translation="MKLVGSYTSPFVRKLSILLLEKGITFEFINELPYNADNGVAQFN
                     PLGKVPVLVTEEGECWFDSPIIAEYIELMNVAPAMLPRDPLESLRVRKIEALADGIMD
                     AGLVSVREQARPAAQQSEDELLRQREKINRSLDVLEGYLVDGTLKTDTVNLATIAIAC
                     AVGYLNFRRVAPGWCVDRPHLVKLVENLFSRESFARTEPPKA"
     gene            1874..3265
                     /gene="selA"
                     /locus_tag="ACHOSC_RS21150"
                     /old_locus_tag="pgaptmp_004230"
     CDS             1874..3265
                     /gene="selA"
                     /locus_tag="ACHOSC_RS21150"
                     /old_locus_tag="pgaptmp_004230"
                     /EC_number="2.9.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004125 - L-seryl-tRNA(Sec) selenium
                     transferase activity [Evidence IEA];
                     GO_process: GO:0001514 - selenocysteine incorporation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-seryl-tRNA(Sec) selenium transferase"
                     /protein_id="HGU4550043.1"
                     /translation="MTTETRSLYSQLPAIDRLLRDSSFLSLRDTYGHTRVVELLRQML
                     DEAREVIRGSQTLPAWCENWAQEVDARLTKEAQSALRPVINLTGTVLHTNLGRALQAE
                     AAVEAVAQAMRSPVTLEYDLDDAGRGHRDRALAQLLCRITGAEDACIVNNNAAAVLLM
                     LAATASGKEVVVSRGELVEIGGAFRIPDVMRQAGCTLHEVGTTNRTHANDYCQAVNEN
                     TALLMKVHTSNYSIQGFTKAIDEAELVALGKELDVPVVTDLGSGSLVDLSQYGLPKEP
                     MPQELIAAGVSLVSFSGDKLLGGPQAGIIVGKKEMIARLQSHPLKRALRADKMTLAAL
                     EATLRLYLHPEALSEKLPTLRLLTRSAEVIQIQAQRLQAPLAAHYGAEFAVQVMPCLS
                     QIGSGSLPVDRLPSAALTFTPHDGRGSHLESLAARWRELPVPVIGRIYDGRLWLDLRC
                     LEDEQRFLEMLLK"
     gene            3262..>5070
                     /gene="selB"
                     /locus_tag="ACHOSC_RS21155"
                     /old_locus_tag="pgaptmp_004231"
     CDS             3262..>5070
                     /gene="selB"
                     /locus_tag="ACHOSC_RS21155"
                     /old_locus_tag="pgaptmp_004231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0001514 - selenocysteine incorporation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="selenocysteine-specific translation elongation
                     factor"
                     /protein_id="HGU4550044.1"
                     /translation="MIIATAGHVDHGKTTLLQAITGVNADRLPEEKKRGMTIDLGYAY
                     WPQPDGRVPGFIDVPGHEKFLSNMLAGVGGIDHALLVVACDDGVMAQTREHLAILQLT
                     GNPMLTVVLTKADRVDEARVDEVERQVKEVLREYGFAEAKLFITAATEGRGIDALREH
                     LLQLPEREHASQHSFRLAIDRAFTVKGAGLVVTGTALSGEVKVGDSLWLTGVNKPMRV
                     RALHAQNQPTETAHAGQRIALNIAGDAEKEQINRGDWLLADAPPEPFTRVIVELQTHT
                     PLTQWQPLHIHHAASHVTGRVSLLEDNLAELVFDTPLWLADNDRLVLRDISARNTLAG
                     ARVVMLNPPRRGKRKPEYLQWLASLARAQSDADALSVHLERGAVNLADFAWARQLNGE
                     GMRELLQQPGYIQAGYSLLNAPVAARWQRKILDTLATYHEQHRDEPGPGRERLRRMAL
                     PMEDEALVLLLIEKMRESGDILSHHGWLHLPDHKAGFSEEQQAIWQKAEPLFGDEPWW
                     VRDLAKETGTDEQAMRLTLRQAAQQGIITAIVKDRYYRNDRIVEFANMIRDLDQECGS
                     TCAADFRDRLGVGRKLAIQILEYFDRIGFTRRRGNDH"
BASE COUNT         1090 a         1359 c         1513 g         1108 t
ORIGIN      
        1 gcgcaccagc ggcgcggcag gtaaattctc cgggtattcc gggtcgtgcg tcagccacac
       61 ggcagacaga cggatgccgt tgtcccggcc atattcggtg taacccggca gggtatcagg
      121 aaaagcggac ggttccgtcc cgccggaaat ggcctgctgc cgggcttctt ctgcccgctg
      181 cgcctgcgtg gtcagtacca gccggaagtg acgcccggca ccatccgtca cgccggtgat
      241 ttcgccgctg aattcaccgg cggcttcgcg gtggaacgtc tgtgtgcgcc cgaagcggtc
      301 caccagcccg gtcagtaccc ggtacggcgg cagcggcgca ggcagcacct catccgcttc
      361 cggcacccgc tcacaccagc cgagcagcca ccacggcccc tgcggactgt ttgtcgccag
      421 ataacgatgc ggacttaagc ggagttcttc cggcagcgcc tgccagagtg cggccagccg
      481 gtgaccttca tccagtttcg ccacgccgcc gcgcaccagc cacagtgact cgctgcggct
      541 gtaaccgtcc tcaccgggaa acaggtgctc aaaatacagg cttctgccgc cgttatcact
      601 gagtatcagt gtgttatcgc gcagctgtaa gcggatatcc gcaggcattt tccagccggg
      661 gccgaggctc cccaccggcg cgggcgtttt tgtccggtaa ctgctgtagg tgcgggagag
      721 gatgaacggc agcgggccgg gcagggcgat gtcggtttca ccgggaagga cctttgcacc
      781 gagcagggga ttgaccggat ggccggacgt cactccgccg gggcacaccg aacaggccac
      841 gccggtgggg gcaccaatgc gcaccccggc tgaaccctga acaatgctac cgccatactg
      901 cgtcatgtcg ccctgacgcg ccgccggttt tccgctcatc tgctcatcct tgttttatcg
      961 ggttatttgt ctgcttccag gcaaaaccct acagatttat aagaaagcct taagtgagat
     1021 atccatcaga tagaaacaat taggaaaata tttatatcac catgataaat atgtgattta
     1081 tttggaagta acaaaccatc aacattttct gccctttttc ccatcaccag gccatactga
     1141 tatttttcct cgcacaaagg actcggcatg aaactcgtcg gtagctacac cagcccgttt
     1201 gtacgcaaac tttctattct gttgttagaa aagggcataa ctttcgaatt tattaatgaa
     1261 ctgccctata acgcggacaa cggcgtggcg caatttaacc cgttaggaaa agtgccggtg
     1321 ctggtgaccg aagagggcga atgttggttt gattcgccga tcatcgctga atatattgaa
     1381 ttaatgaatg tcgctccggc gatgttgccg cgcgatccgc tggagtcgtt gcgggtgcgc
     1441 aaaattgagg cactggcgga tggcattatg gatgccgggc tggtatcggt gcgtgaacag
     1501 gcgcgtccag cggcgcagca gtctgaagat gaattgttac gccagcggga gaaaatcaac
     1561 cgcagtctgg atgtgctgga aggatatctg gtcgatggca cactcaaaac cgatacggtc
     1621 aatctggcga ctatcgccat tgcctgtgcc gtcggatatc tcaatttccg ccgcgttgcg
     1681 ccaggctggt gtgtcgatcg cccacactta gtcaaactgg tcgaaaacct gtttagccgc
     1741 gaaagttttg ctcgcaccga accgccaaag gcttgatgcg cgatatgtcc tcctgaccca
     1801 tctcacgtta caatccgtgg ttatgttaaa cgcccttctc cgtgtgagag ggccttgatc
     1861 agccaggttt cctatgacaa ccgaaacgcg ttccctctat agtcaacttc cggctattga
     1921 tcgcttattg cgcgatagct ccttcctttc tttgcgtgat acttatggtc acacccgcgt
     1981 ggtggaattg ttgcgtcaga tgctcgacga agcgcgagaa gtgattcgtg gcagccagac
     2041 gctgcctgcg tggtgtgaaa actgggcgca agaagtcgat gcccggttga cgaaagaagc
     2101 gcagagcgcg ctgcgtccgg tgatcaacct gacgggaacc gtgctgcata ccaaccttgg
     2161 gcgagcttta caggcggaag ccgcggtgga agccgttgcg caggctatgc gttcgccagt
     2221 gaccctcgag tacgatctgg acgacgctgg acgcggacat cgcgatcgtg cgctggcgca
     2281 gttgctgtgc cgcattacgg gggcggaaga tgcctgtatc gttaataaca atgcggcggc
     2341 ggtgttattg atgttggcgg ccactgccag cgggaaagag gtggtggttt ctcgcggcga
     2401 actggtggag attggcggcg cgtttcgtat tcccgatgtt atgcgtcagg caggctgcac
     2461 cctacacgaa gtagggacca ccaaccgcac gcacgcgaat gattattgtc aggcggtgaa
     2521 tgaaaatacc gcactgttga tgaaagtaca taccagtaac tacagcattc aggggttcac
     2581 caaagcgata gatgaagcgg aactggtggc gctcggcaaa gagctggatg ttcccgtagt
     2641 gactgattta ggcagtggct cgctggtcga tcttagccag tacggtttgc cgaaagagcc
     2701 aatgccgcag gagttgattg cggcgggcgt cagtctggtg agtttctccg gcgacaagtt
     2761 gttaggcggg ccgcaggcag gaattattgt tggtaaaaaa gagatgatcg cccgcctgca
     2821 aagccacccg ctgaagcgtg cattacgcgc ggataaaatg accctcgcgg cgctggaagc
     2881 cacgttgcgt ctttatttac accctgaagc tctgagtgaa aaattaccga ccctgcgcct
     2941 gcttacccgc agcgcagagg tcattcaaat ccaggcacaa cgtttacagg ccccccttgc
     3001 cgcacattac ggcgcggagt ttgcggtaca ggttatgcca tgtctttcgc agattggcag
     3061 tggttcgctg ccggttgatc gcctgccgag cgcggcatta acgtttacac cccatgatgg
     3121 acgcggtagc caccttgagt cattagccgc ccgctggcgt gaattgccag tgccggtgat
     3181 tggtcgtatt tatgacggac gattgtggct ggatttacgc tgccttgaag atgagcaacg
     3241 gtttttggag atgttgttga aatgattatt gcgactgccg gacacgttga ccacggcaaa
     3301 acaaccttat tgcaggcgat tactggcgta aatgctgacc gtctgccgga agaaaaaaag
     3361 cgcggcatga ccatcgatct cggctatgcc tactggccgc agccggatgg tcgcgtgcct
     3421 ggttttatcg acgttcccgg tcatgaaaaa tttctttcca acatgctggc gggcgttggt
     3481 ggtatcgatc acgcgctgtt ggtggtggcg tgcgatgacg gtgtgatggc acagacccgt
     3541 gagcatctgg cgattttgca gctgaccggt aacccgatgc tgacagtggt gctgaccaaa
     3601 gccgatcgcg tggacgaagc gcgtgttgat gaggttgaac gtcaggtaaa ggaggtgctg
     3661 cgggaatacg gttttgctga ggcaaaactg tttatcaccg cagcaaccga aggtcgagga
     3721 attgatgccc tgcgcgagca tctgcttcag ttaccagaac gtgaacacgc cagccaacat
     3781 agtttccgcc tcgcgatcga ccgcgcattt accgtaaaag gtgccgggct ggtcgtcacc
     3841 ggtacggcgt taagcgggga agtgaaggta ggcgattcac tatggctaac tggtgtaaat
     3901 aaaccgatgc gtgtacgtgc gctgcatgca caaaaccagc caacagaaac cgcccatgcc
     3961 gggcagcgta tcgcgcttaa catcgcgggt gatgcggaaa aagagcagat taaccgtggc
     4021 gactggctgc ttgccgatgc gccgccagag ccgttcacac gggtgattgt cgagcttcaa
     4081 acccatacgc cgctgaccca gtggcagccg ctgcatattc accacgccgc cagccacgtt
     4141 acgggacgcg tttcgctgct ggaagataac cttgccgagc tggtgttcga cacgccgctg
     4201 tggctggcgg ataacgatcg cttagtgctg cgcgatatct cggcccgcaa cacgctggcg
     4261 ggggcgcgtg tggtgatgct taacccgccg cgtcgcggta aacgtaagcc ggaatatctg
     4321 caatggctgg cgtctcttgc acgggcgcag agcgatgccg atgcgttatc tgttcatctg
     4381 gaacgcggcg cggttaacct tgcggatttc gcctgggcgc gccagctcaa cggcgaaggg
     4441 atgcgcgaat tgctgcaaca gcctggttat attcaggctg gttatagctt gttgaatgcg
     4501 ccggttgccg cccgctggca gcggaaaatt ctcgacacat tagcgactta tcatgagcaa
     4561 catcgcgatg aacctggtcc tgggcgcgaa cgtctgcgac gtatggcgtt gccaatggaa
     4621 gatgaagcgc tggtactgtt gctgattgaa aagatgcgcg aaagcggcga catcctcagt
     4681 catcacggtt ggctgcatct gccggatcac aaagcgggct tcagcgaaga gcagcaggcc
     4741 atctggcaaa aagcagagcc gctgtttggt gacgagccgt ggtgggtgcg tgatctggca
     4801 aaagagacgg gtaccgacga gcaggcaatg cgcctgacgc tacgccaggc ggcgcagcaa
     4861 ggaataatta ccgcgatcgt caaagatcgt tattaccgta acgatcggat tgtcgaattt
     4921 gccaatatga tccgcgatct cgatcaggag tgtggttcaa cctgcgcggc ggatttccgc
     4981 gatcgcttag gcgtaggccg aaagctggca attcagattc tggaatattt tgaccgcatt
     5041 ggctttacgc gtcgtcgtgg aaatgatcat
//
LOCUS       DAZKHF020000284         5065 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2201S31241
            SAMN43267800-rid25106413.denovo.286, whole genome shotgun sequence.
ACCESSION   DAZKHF020000284 DAZKHF020000000
VERSION     DAZKHF020000284.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267800
            Sequence Read Archive: SRR30307263
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5065)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5065)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 5065)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350581.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350581.2
            Genome Coverage       :: 151x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:21:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,652
            CDSs (total)                      :: 5,557
            Genes (coding)                    :: 5,320
            CDSs (with protein)               :: 5,320
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 237
            CDSs (without protein)            :: 237
            Pseudo Genes (ambiguous residues) :: 0 of 237
            Pseudo Genes (frameshifted)       :: 102 of 237
            Pseudo Genes (incomplete)         :: 130 of 237
            Pseudo Genes (internal stop)      :: 52 of 237
            Pseudo Genes (multiple problems)  :: 41 of 237
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5065
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267800-rid25106413.denovo.286"
                     /serotype="O 26:H11"
                     /isolate="2201S31241"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-01"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            135..437
                     /locus_tag="ACHOSC_RS21160"
                     /old_locus_tag="pgaptmp_004232"
     CDS             135..437
                     /locus_tag="ACHOSC_RS21160"
                     /old_locus_tag="pgaptmp_004232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004142601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HigB family
                     toxin"
                     /protein_id="HGU4550045.1"
                     /translation="MKIISVKTLRDFWAENPDAEQPLRAWVDEAAKADWKSPADIKAQ
                     YRTASILKNRRVVFNIKGNHYRLIVAIAYQRGWVFVKFIGNHKEYDAIDADTIELE"
     gene            440..799
                     /locus_tag="ACHOSC_RS21165"
                     /old_locus_tag="pgaptmp_004233"
     CDS             440..799
                     /locus_tag="ACHOSC_RS21165"
                     /old_locus_tag="pgaptmp_004233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007374288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HGU4550046.1"
                     /translation="MNIKPIRTEQDYEAALRAVEPMFDNEPEIDTPEGDFFEVMCLLI
                     EEYEKKHYPIEPPSPIEAIRFRMEQQGLTVKDLEPAIGKKNRVYEVLNGTRSLTLPMI
                     RRLHNQFGIPLESLVGL"
     gene            complement(846..1238)
                     /locus_tag="ACHOSC_RS21170"
                     /old_locus_tag="pgaptmp_004234"
     CDS             complement(846..1238)
                     /locus_tag="ACHOSC_RS21170"
                     /old_locus_tag="pgaptmp_004234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000578360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HGU4550047.1"
                     /translation="MIFVKRLQQVLQELNINQSELGRRLGVKPQSVQGWLKGVMPRMD
                     KLEKLAELSQHPVHWFFMEEETLGDKMAVSSNDNQPQLTEQQRKIISLLDELPQSDAE
                     QIIRDMEQKRDFYKRKLEELLRQKNKTA"
     gene            1365..1625
                     /locus_tag="ACHOSC_RS21175"
                     /old_locus_tag="pgaptmp_004235"
     CDS             1365..1625
                     /locus_tag="ACHOSC_RS21175"
                     /old_locus_tag="pgaptmp_004235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001172789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HGU4550048.1"
                     /translation="MQKSTQVKILSIMSQSELGRRLGKTPQTISGWFKKRVPAEEVIP
                     ACEALDWGVTPHELRPDKYPNPTDGLPVEYQANAQAAAGVDS"
     gene            1622..2059
                     /locus_tag="ACHOSC_RS21180"
                     /old_locus_tag="pgaptmp_004236"
     CDS             1622..2059
                     /locus_tag="ACHOSC_RS21180"
                     /old_locus_tag="pgaptmp_004236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000693932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxin YdaT family protein"
                     /protein_id="HGU4550049.1"
                     /translation="MKIKHEHIRMAMNAWLLYPRVGRKKIADDIATAYFELEMTYPPM
                     HDTSTTEGIGLNIQNIFRWLEKDTPDAVEKIQALIPAILTVLPRELRYHLSIFDTVER
                     RALLAAQEALSTAIDAHDDAVQAVYRKAYFSDGGSSGESVVVH"
     gene            2146..3156
                     /locus_tag="ACHOSC_RS21185"
                     /old_locus_tag="pgaptmp_004237"
     CDS             2146..3156
                     /locus_tag="ACHOSC_RS21185"
                     /old_locus_tag="pgaptmp_004237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001774823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1376 domain-containing protein"
                     /protein_id="HGU4550050.1"
                     /translation="MAALPYMQLYIADYLADTMHLSAEEHGAYLLLMFNYWQTGKPIP
                     KNRLAKISRLTNERWADVEPSLREFFCDNSDEWVHLRIEEDLASVREKLTKKSAAGKA
                     SVQARRSRKEAYVQTKQERDLTGVQTNVGVVFEHDANTKATNKDTDLKELNPTHNVRE
                     RESIPTSESHGAPLQTAEPEYLDGLSEPIGKFSMTTVWQPSPDFRQRAAVWGMALPEP
                     EFTPAELAAFRDYWMAEGKVFTQVQWEQKFARHVQHVRAQVKPVSKGVSHAASGGTAS
                     RAVQEIRAAREQWERENGFISNGNGLEAVGAYGGGVFEPLDSEERGRTFEALDCPDWC
                     DD"
     gene            3149..3610
                     /locus_tag="ACHOSC_RS21190"
                     /old_locus_tag="pgaptmp_004238"
     CDS             3149..3610
                     /locus_tag="ACHOSC_RS21190"
                     /old_locus_tag="pgaptmp_004238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310232.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replication protein P"
                     /protein_id="HGU4550051.1"
                     /translation="MTEQQIRLVCRQCMDRCRAGETWPPDLAEFVALISESGANPFGL
                     TVDAVMEEYRRWRNESWRYDGSDKYPWPQPVLYHICLEMRTRGIERQMTQGELKLLAE
                     RQLTKWAKHVGNGMSVPPVRRQLEGAKHPQGPTPIERLKQEYERRKAAGFI"
     gene            3644..4369
                     /locus_tag="ACHOSC_RS21195"
                     /old_locus_tag="pgaptmp_004239"
     CDS             3644..4369
                     /locus_tag="ACHOSC_RS21195"
                     /old_locus_tag="pgaptmp_004239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000450647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1627 domain-containing protein"
                     /protein_id="HGU4550052.1"
                     /translation="METVFDALKALKRASSQVVAARLGISREDAVNELWKLKRRGEAD
                     NKGSMWWLIQAGESEPVSPVPKVTAQMLTEAIEQHGPQTADELALMFGITSRRANSSL
                     AMVISKGRLIRVNQGGKFRYCIPGADLPAEPEAASVAETDGKAFPQPAGVALPVREAE
                     TQEEIKTESVAVTVQSQPSFTRKHPDGLILPSLHVANRELRRAKGQVQKWERVCAALR
                     ELNKHRDIVRQITDSSRRVVSEK"
     gene            4385..4777
                     /locus_tag="ACHOSC_RS21200"
                     /old_locus_tag="pgaptmp_004240"
     CDS             4385..4777
                     /locus_tag="ACHOSC_RS21200"
                     /old_locus_tag="pgaptmp_004240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001141097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF977 family protein"
                     /protein_id="HGU4550053.1"
                     /translation="MAKEFTPEEREEVKARIVEFVRLSGRETFRQLADKTGVSKTAIR
                     RLSGALAASGDVWLSGCGVFPSEQAYRVWRKTPEKAADPTLIRKLPDGEIRRYNRRQN
                     IICRECRQSEVMQRVLAFYRGNFQEVME"
     gene            4774..>5065
                     /locus_tag="ACHOSC_RS21205"
                     /old_locus_tag="pgaptmp_004241"
     CDS             4774..>5065
                     /locus_tag="ACHOSC_RS21205"
                     /old_locus_tag="pgaptmp_004241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001266128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4406 domain-containing protein"
                     /protein_id="HGU4550054.1"
                     /translation="MRVRVYIAGPMTGYENFNREAFHKAEEELKREGHTVLTPAVLPD
                     GLTQPHYMDICMAMIRCVDAIYMLNGWQRSAGAKAELALAEKLGHAVIYQEVA"
BASE COUNT         1385 a         1081 c         1409 g         1190 t
ORIGIN      
        1 ccctgcgttc tgtggggcgg ggtgggttta tggatacaat ttacaaatta aaatttaact
       61 ggtcaacatg tttattatta aatttgtaat tgcgggctgt tgcatcagtc ccaaaatggg
      121 actactatac agttatgaag attatctcag ttaaaacact cagggatttt tgggcggaga
      181 atcctgatgc agaacaaccg ctaagggcat gggtggatga ggcggcaaaa gctgactgga
      241 aaagcccagc agacattaag gcacaatacc gaacggccag tatattgaaa aaccggcgcg
      301 tggtattcaa cataaaaggt aatcattacc gtttaattgt tgcaattgcg tatcagcgag
      361 gatgggtatt tgttaaattc atcggaaacc acaaggaata cgatgctatt gatgctgata
      421 ccatcgaact ggagtaagca tgaacatcaa acctattcgt acagagcaag attatgaagc
      481 cgcgttgcgt gctgttgaac ccatgttcga caatgagccc gaaattgaca cgcctgaggg
      541 ggattttttt gaggtgatgt gtttgctaat agaggaatat gaaaaaaaac attatcccat
      601 tgagccacca tccccaattg aagctataag atttcgcatg gaacagcagg ggctgactgt
      661 gaaagatttg gaacccgcaa ttgggaaaaa aaatcgggtt tatgaggtgt tgaatggtac
      721 cagaagcctt acgttaccaa tgattcgccg tcttcataat caatttggta tccccctgga
      781 aagcctggtt ggattataaa atctgctagt catttgcctg atgctcgttc cagaaaagga
      841 atgcatcagg cagttttgtt tttctgccgc agtaactctt caagtttccg tttatagaaa
      901 tcgcgttttt gctccatatc acgaatgatc tgctctgcgt cgctttgagg taactcatct
      961 aaaagcgata tgatttttcg ttgttgttct gtaagttgcg gttggttgtc attactggat
     1021 acagccattt tatcgccgag agtttcttct tccataaaga accaatggac gggatgttgt
     1081 gagagctctg ctaatttttc cagtttatcc attcttggca tcacgccttt caaccaacct
     1141 tgcacggatt ggggttttac accaagacgt cttcccagct ctgactggtt tatattcaat
     1201 tcctgcaaca cctgctgaag gcgttttaca aagatcatca ccacccctcg taaactagtt
     1261 ccgcgatcct acagaaaaaa ttgataagtg gcattacaaa tagaagttga aatttaaatt
     1321 taaatttgta attatcggtg tcatcgtaaa gttcggaggg aaacatgcaa aaaagtactc
     1381 aagtgaaaat cctgtcaata atgagccaat cagaattagg gcgtcgtctt ggtaaaacac
     1441 cgcaaaccat aagtgggtgg tttaaaaaac gagtgccagc ggaggaggtt ataccagcat
     1501 gtgaggcgct tgactgggga gtaactccgc atgaattgcg ccctgataaa taccctaatc
     1561 caaccgatgg tttacctgtt gagtatcagg ctaacgcaca agcagcggcg ggagttgatt
     1621 catgaaaatc aagcatgagc acatccgcat ggcgatgaat gcgtggctgc tttatccgag
     1681 ggtagggcgc aaaaaaatcg ctgatgatat agcgacagca tattttgagc ttgaaatgac
     1741 ttatccacca atgcatgaca cctctacgac agagggtatt ggattgaaca tacaaaatat
     1801 ttttcgctgg cttgaaaagg atacgcctga tgctgttgaa aaaattcagg cactaattcc
     1861 agctatcctg actgttcttc cgcgtgaact gcgttatcac ctcagtattt ttgacactgt
     1921 tgagcgccgt gcattactgg cggcccagga agcgttgagc acggcaattg atgcgcatga
     1981 tgatgcagtc caggcagttt accggaaagc gtatttcagc gacggcgggt catccggcga
     2041 gtctgttgtg gtgcattgat atttatgcca gacccctgct gattctgttg attggggaat
     2101 cacagaatat caccagagga tggttcgtca caagatgagg caattatggc cgcattacca
     2161 tacatgcaac tgtacatagc tgattacctg gctgacacca tgcatttgtc cgcagaggag
     2221 cacggtgcgt atttgttgct gatgttcaat tactggcaaa cagggaaacc aatacctaaa
     2281 aacaggctgg caaaaatttc ccgtctgact aacgagcgat gggctgatgt ggaaccatcc
     2341 ttgcgggagt ttttttgcga taacagcgat gaatgggtgc atcttcggat tgaggaagat
     2401 ctggcatcag tcagggaaaa attgaccaaa aaatcagccg ccggaaaagc atctgttcag
     2461 gccagaagaa gcagaaagga agcatatgtt caaacaaaac aagagagaga tttaacaggt
     2521 gttcaaacaa atgttggtgt tgtgtttgaa catgacgcaa acacaaaagc aactaataaa
     2581 gatacagatc taaaagaatt aaaccccaca cataacgtgc gtgaacgcga gagtattccg
     2641 accagtgagt cgcatggtgc gccgttgcag acagccgaac ctgaatacct ggacggcctg
     2701 agcgaaccga tcgggaaatt ttcgatgact actgtctggc agccgtcgcc ggattttcga
     2761 caacgggcag cagtgtgggg tatggctctg cctgagccgg aatttacacc tgctgagctt
     2821 gccgcattcc gggattactg gatggcggag gggaaggttt tcacgcaggt tcagtgggag
     2881 cagaaatttg cccgccacgt gcagcacgtc agggcacagg taaaaccagt cagcaagggg
     2941 gtaagccatg cagcatcagg tggcacggca tcacgggcag ttcaggaaat ccgggcagca
     3001 cgcgaacagt gggaacgtga aaacggattt atcagcaacg gaaacggcct ggaagctgtg
     3061 ggagcttatg ggggaggtgt attcgaaccg ctggactcag aagaacgggg ccgcaccttc
     3121 gaagctctgg attgcccaga ttggtgcgat gactgaacag caaatccgtc tggtctgccg
     3181 tcagtgcatg gaccgctgcc gggcgggtga aacgtggccc ccggacctgg ctgagtttgt
     3241 tgcgctgatt tcggagagtg gggcaaatcc atttggtctt acggtggatg ccgtgatgga
     3301 agagtaccgg cgctggcgca atgaatcctg gcgatacgac gggagtgata aatacccgtg
     3361 gccacagcct gtgctgtacc acatctgcct cgaaatgcgt accagaggga ttgagcgcca
     3421 gatgacgcag ggtgagttaa aactacttgc agaacggcaa ctgacgaaat gggcaaagca
     3481 tgttggtaac gggatgagtg ttccgccagt gcgacgacaa ctggaagggg cgaaacaccc
     3541 gcaagggcca acgccaattg agcggctgaa acaggaatac gaacgccgga aggcagctgg
     3601 ttttatttga atctgagaaa cgattttgtc ggaggaaatt ttaatggaaa ccgtatttga
     3661 cgcactgaaa gcactgaaaa gagcctcttc acaggtagtg gcggcccgcc ttggaatcag
     3721 ccgtgaagat gcggtcaacg aactgtggaa actgaagcgc cgcggtgaag cggataacaa
     3781 ggggtcgatg tggtggctga ttcaggctgg tgaaagtgaa ccggtgtcac cggtaccgaa
     3841 agtgacagcg caaatgctga ctgaggcgat tgaacaacat ggcccacaaa cggcggatga
     3901 gctggcactg atgttcggga ttacctcccg ccgggcgaat tcatcgctgg ccatggtaat
     3961 cagcaaaggg cgtctgattc gcgtgaatca gggcggtaaa tttcgttact gcataccggg
     4021 cgctgattta ccggcagagc cggaagctgc atccgtagcg gaaaccgatg gtaaagcctt
     4081 tcctcagcca gcaggtgttg cgttaccagt tcgggaagca gaaacacagg aagaaataaa
     4141 aacggaaagt gtggcggtca cagtgcagtc acagccgtcg ttcaccagaa aacatccgga
     4201 tggtctgatt ttaccatcgc tgcatgtggc taaccgcgag ctgcgccggg caaaaggtca
     4261 ggttcagaag tgggagcgag tctgcgccgc gctgcgggag ctgaacaagc accgggatat
     4321 tgttcgacag attactgatt cttcccgccg tgttgtatcg gaaaagtgat tgccggaggc
     4381 gcttatggca aaagaattta caccagaaga gcgggaagaa gtgaaggcgc gcattgtgga
     4441 attcgtgcgc ctgagcggac gagaaacttt tcgacaactg gcagataaaa cgggtgtcag
     4501 taagaccgct attcgtcgtt tatctggtgc gcttgcggcc agtggtgatg tctggctctc
     4561 tggttgcggg gtatttccat cagagcaggc gtatcgcgta tggcgtaaga caccggagaa
     4621 ggctgctgac ccgacactga ttcgaaagtt acctgacgga gaaatacgtc gttacaacag
     4681 acggcagaac ataatttgtc gtgagtgccg ccagagcgaa gttatgcagc gtgtgctggc
     4741 gttctatcgg ggaaactttc aggaggtgat ggagtgaggg tgagagttta tattgccggt
     4801 ccaatgacgg gatatgaaaa tttcaaccgt gaggcgtttc acaaggcgga agaggaactg
     4861 aaacgggaag ggcataccgt cttaactccg gcagtacttc cggacgggct gacacagccg
     4921 cactacatgg atatttgcat ggcaatgatt cgttgtgtgg atgcgattta catgctgaat
     4981 ggctggcagc ggtcagcggg cgctaaggca gagctggcac tggcggagaa actggggcat
     5041 gcggtgattt atcaggaggt ggctc
//
LOCUS       DAZKHF020000285         5041 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2201S31241
            SAMN43267800-rid25106413.denovo.287, whole genome shotgun sequence.
ACCESSION   DAZKHF020000285 DAZKHF020000000
VERSION     DAZKHF020000285.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267800
            Sequence Read Archive: SRR30307263
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5041)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5041)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 5041)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350581.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350581.2
            Genome Coverage       :: 151x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:21:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,652
            CDSs (total)                      :: 5,557
            Genes (coding)                    :: 5,320
            CDSs (with protein)               :: 5,320
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 237
            CDSs (without protein)            :: 237
            Pseudo Genes (ambiguous residues) :: 0 of 237
            Pseudo Genes (frameshifted)       :: 102 of 237
            Pseudo Genes (incomplete)         :: 130 of 237
            Pseudo Genes (internal stop)      :: 52 of 237
            Pseudo Genes (multiple problems)  :: 41 of 237
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5041
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267800-rid25106413.denovo.287"
                     /serotype="O 26:H11"
                     /isolate="2201S31241"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-01"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            <1..1328
                     /gene="garP"
                     /locus_tag="ACHOSC_RS21210"
                     /old_locus_tag="pgaptmp_004242"
     CDS             <1..1328
                     /gene="garP"
                     /locus_tag="ACHOSC_RS21210"
                     /old_locus_tag="pgaptmp_004242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="galactarate/glucarate/glycerate transporter
                     GarP"
                     /protein_id="HGU4550055.1"
                     /translation="DTVDEKKKGVHTRYLILLIIFIVTAVNYADRATLSIAGTEVAKE
                     LQLSAVSMGYIFSAFGWAYLLMQIPGGWLLDKFGSKKVYTYSLFFWSLFTFLQGFVDM
                     FPLAWAGISMFFMRFMLGFSEAPSFPANARIVAAWFPTKERGTASAIFNSAQYFSLAL
                     FSPLLGWLTFAWGWEHVFTVMGVIGFVLTALWIKLIHNPTDHPRMSAEELKFISENGA
                     VVDMDHKKPGSAAASGPKLHYIKQLLSNRMMLGVFFGQYFINTITWFFLTWFPIYLVQ
                     EKGMSILKVGLVASIPALCGFAGGVLGGVFSDYLIKRGLSLTLARKLPIVLGMLLAST
                     IILCNYTNNTTLVVMLMALAFFGKGFGALGWPVISDTAPKEIVGLCGGVFNVFGNVAS
                     IVTPLVIGYLVSELHSFNAALVFVGCSALMAMVCYLFVVGDIKRMELQK"
     gene            1344..2114
                     /gene="garL"
                     /locus_tag="ACHOSC_RS21215"
                     /old_locus_tag="pgaptmp_004243"
     CDS             1344..2114
                     /gene="garL"
                     /locus_tag="ACHOSC_RS21215"
                     /old_locus_tag="pgaptmp_004243"
                     /EC_number="4.1.2.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008672 - 2-dehydro-3-deoxyglucarate
                     aldolase activity [Evidence IEA];
                     GO_process: GO:0019394 - glucarate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydro-3-deoxyglucarate aldolase"
                     /protein_id="HGU4550056.1"
                     /translation="MNNDVFPNKFKAALAAKQVQIGCWSALSNPISTEVLGLAGFDWL
                     VLDGEHAPNDISTFIPQLMALKGSASAPVVRVPTNEPVIIKRLLDIGFYNFLIPFVET
                     KEEAEQAVASTRYPPEGIRGVSVSHRANMFGTVADYFAQSNKNITILVQIESQQGVDN
                     VDAIAATEGVDGIFVGPSDLAAALGHLGNASHPDVQKAIQHIFNRASAHGKPSGILAP
                     VEADARRYLEWGATFVAVGSDLGVFRSATQKLADTFKK"
     gene            2144..3034
                     /gene="garR"
                     /locus_tag="ACHOSC_RS21220"
                     /old_locus_tag="pgaptmp_004244"
     CDS             2144..3034
                     /gene="garR"
                     /locus_tag="ACHOSC_RS21220"
                     /old_locus_tag="pgaptmp_004244"
                     /EC_number="1.1.1.60"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312030.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008679 - 2-hydroxy-3-oxopropionate
                     reductase activity [Evidence IEA];
                     GO_process: GO:0046487 - glyoxylate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxy-3-oxopropionate reductase"
                     /protein_id="HGU4550057.1"
                     /translation="MTMKVGFIGLGIMGKPMSKNLLKAGYSLVVADRNPEAIADVIAA
                     GAETASTAKAIAEQCDVIITMLPNSPHVKEVALGENGIIEGAKPGTVLIDMSSIAPLA
                     SREISEALKAKGIDMLDAPVSGGEPKAIDGTLSVMVGGDKAIFDKYYDLMKAMAGSVV
                     HTGEIGAGNVTKLANQVIVALNIAAMSEALTLATKAGVNPDLVYQAIRGGLAGSTVLD
                     AKAPMVMDRNFKPGFRIDLHIKDLANALDTSHGVGAQLPLTAAVMEMMQALRADGLGT
                     ADHSALACYYEKLAKVEVTR"
     gene            3131..4276
                     /gene="garK"
                     /locus_tag="ACHOSC_RS21225"
                     /old_locus_tag="pgaptmp_004245"
     CDS             3131..4276
                     /gene="garK"
                     /locus_tag="ACHOSC_RS21225"
                     /old_locus_tag="pgaptmp_004245"
                     /EC_number="2.7.1.165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312029.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008887 - glycerate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0031388 - organic acid phosphorylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerate 2-kinase"
                     /protein_id="HGU4550058.1"
                     /translation="MKIVIAPDSYKESLSASEVAQAIEKGFREIFPDAQYVSIPVADG
                     GEGTVEAMIAATQGSERHAWVTGPLGEKVNASWGISGDGKTAFIEMAAASGLELVPAE
                     KRDPLVTTSRGTGELILQALESGATNIIIGIGGSATNDGGAGMVQALGAKLCDANGNE
                     IGFGGGSLNTLNDIDISGLDPRLKDCVIRVACDVTNPLVGDNGASRIFGPQKGASEAM
                     IVELDNNLSHYAEVIKKALHVDVKDVPGAGAAGGMGAALMAFLGAELKSGIEIVTTAL
                     NLEEHIHDCTLVITGEGRIDSQSIHGKVPIGVANVAKKYHKPVIGIAGSLTDDVGVVH
                     QHGIDAVFSVLTSIGTLDEAFRGAYDNICRASRNIAATLAIGMRNAG"
     gene            4309..4685
                     /gene="rnpB"
                     /locus_tag="ACHOSC_RS21230"
                     /old_locus_tag="pgaptmp_004246"
     ncRNA           4309..4685
                     /ncRNA_class="RNase_P_RNA"
                     /gene="rnpB"
                     /locus_tag="ACHOSC_RS21230"
                     /old_locus_tag="pgaptmp_004246"
                     /product="RNase P RNA component class A"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00010"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00010"
     gene            complement(4855..>5041)
                     /locus_tag="ACHOSC_RS21235"
                     /old_locus_tag="pgaptmp_004247"
     CDS             complement(4855..>5041)
                     /locus_tag="ACHOSC_RS21235"
                     /old_locus_tag="pgaptmp_004247"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4550059.1"
                     /translation="DIYSSYPVHQKQKLTDAGLKLMATAKMPDALRLSGLRHLCNILN
                     LRAFVGRIRRSRRIRHE"
BASE COUNT         1164 a         1214 c         1457 g         1206 t
ORIGIN      
        1 tggacaccgt tgacgaaaaa aagaaaggcg tgcatacccg ctatttaata ttactgatta
       61 tttttattgt taccgccgtt aactacgccg atcgtgcaac gctgtctatt gctggtaccg
      121 aagtggcaaa agagttgcag ttaagtgcgg tttcgatggg ttacatcttt tctgcttttg
      181 gctgggccta cttgctgatg caaatccccg gcggctggct gcttgataag tttggctcga
      241 aaaaagttta tacctatagc ctctttttct ggtcgctatt cactttcctg caaggctttg
      301 ttgatatgtt cccgctggcc tgggcaggga tctccatgtt ctttatgcgc tttatgctcg
      361 gcttctcgga agcgccatca ttcccggcga acgcccgtat tgtcgccgcc tggttcccga
      421 cgaaagaacg tggtactgcc tccgccatct ttaactcggc gcaatatttc tcgctggcgc
      481 tcttttcgcc tctgcttggc tggctgactt tcgcctgggg ctgggagcac gtctttaccg
      541 ttatgggggt gattggtttt gtgctgacgg cgctgtggat caaattgatt cataacccga
      601 cagatcaccc acgtatgtct gcggaagagc tgaagtttat ctctgaaaat ggcgcggtgg
      661 tcgatatgga ccacaaaaag ccgggcagcg cggcagcaag cggtcccaaa ctgcattaca
      721 tcaagcaatt gctctctaac cgcatgatgt tgggcgtatt tttcggacaa tattttatca
      781 acaccatcac ctggttcttc ctcacctggt tcccgattta tctggtgcag gaaaaaggca
      841 tgtcgattct gaaagtgggt ctggtcgcct cgattccggc actgtgtggt tttgcgggcg
      901 gcgtgctggg aggtgtcttc tcggattatc tgatcaaacg cggtttatcc ctgaccctgg
      961 cacgtaagct accgattgtg ctgggaatgt tgctggcttc caccatcatc ttatgtaact
     1021 acaccaacaa caccacgctg gtggtcatgc tgatggcgct ggcttttttt ggcaaaggat
     1081 ttggtgctct gggctggccg gtgatttctg acaccgcgcc gaaagagatt gttggcctct
     1141 gtggcggcgt ctttaacgtc tttggcaatg ttgcctccat tgtcactcca ctggtgattg
     1201 gctacctggt aagtgaactg cactccttca atgcagcact ggttttcgtg ggatgttcag
     1261 cgctgatggc gatggtctgc tacctcttcg tagttggcga cattaaacgt atggaattgc
     1321 agaaataagc aaaggtataa gcgatgaata acgatgtttt cccgaataaa ttcaaagccg
     1381 cactggctgc gaaacaggta caaattggtt gctggtcagc actctctaac ccgattagca
     1441 ctgaagttct tggtttggct gggtttgact ggctggtgct ggatggcgaa catgcgccaa
     1501 acgatatctc cacgtttatt ccgcagttaa tggccctgaa aggcagcgcc agcgcgccag
     1561 tagtgcgagt gccgaccaac gagccggtaa ttattaagcg tcttctggat atcggtttct
     1621 ataacttcct gattcctttt gtagaaacaa aagaggaagc agagcaggcg gtggcatcaa
     1681 cccgttaccc accggaaggc attcgcggcg tctccgtttc tcaccgcgcc aatatgtttg
     1741 gcaccgtggc ggattatttc gctcagtcga acaagaacat caccattctg gtccagatag
     1801 aaagtcagca gggcgtagat aacgtcgatg ccattgccgc taccgaaggt gtagacggta
     1861 tcttcgtcgg ccccagcgat ctggctgcgg cattaggcca tctcggcaat gcctcgcacc
     1921 cggatgtaca aaaagcaatt cagcacattt ttaaccgtgc cagtgcgcac ggcaaaccca
     1981 gcggtattct cgcgccggtc gaagccgatg cgcgtcgtta tctggaatgg ggcgcgacgt
     2041 ttgtggctgt cggcagcgat cttggcgtct tccgctctgc cactcagaaa ctggctgata
     2101 cctttaaaaa ataaccacca ccgcaacaag agagatgatt gatatgacta tgaaagttgg
     2161 ttttattggc ctggggatta tgggtaaacc aatgagtaaa aaccttctga aagcaggtta
     2221 ctcgctggtg gttgctgacc gtaacccaga agctattgct gacgtgattg ctgcaggtgc
     2281 agaaacagcg tctacggcta aagcgatcgc tgaacagtgc gacgtcatca taaccatgct
     2341 gccaaactcc cctcatgtga aagaggtggc gctgggtgag aatggcatta ttgaaggcgc
     2401 gaagccaggt acggtattga tcgatatgag ttctatcgca ccgctggcaa gccgtgaaat
     2461 cagcgaagcg ctgaaagcga aaggcattga tatgctggat gctccggtga gcggcggtga
     2521 accgaaagcc atcgacggta cgctgtcagt gatggtgggc ggcgacaagg ctattttcga
     2581 caaatactat gatttgatga aagcgatggc gggttccgtg gtgcataccg gggaaatcgg
     2641 tgcaggtaac gtcaccaaac tggcaaatca ggtcattgtg gcgctgaata ttgccgcgat
     2701 gtcagaagcg ctaacgctgg caaccaaagc gggcgttaac ccggacctgg tgtatcaggc
     2761 aattcgcggt ggactggcgg gcagtaccgt gctggatgcc aaagcgccga tggtgatgga
     2821 ccgcaacttc aagccgggct tccgtattga tctgcatatt aaggatctgg caaatgcgct
     2881 ggatacttcc cacggcgtcg gcgcgcaact gccgctcaca gctgcggtta tggagatgat
     2941 gcaggcactg cgagcagatg gtttaggaac ggcggatcat agcgccctgg cgtgctacta
     3001 cgaaaaactg gcgaaagtcg aagttactcg ttaatgacgt cgtgcccgga tggcgtattg
     3061 caatccgggc ctggaatcca ggccgaataa gagaaacgcc ctccggcgtc atgtggtaac
     3121 aggcataggt atgaaaatcg taatcgcccc agactcttat aaagaaagtt tatctgccag
     3181 cgaggttgcg caggcgatag aaaaaggatt tcgggaaatt tttcctgatg cacagtacgt
     3241 ttctattccg gttgccgacg gtggcgaagg aacggtggaa gcgatgattg cagccaccca
     3301 gggttctgaa cgtcacgcct gggttacagg gccgctgggc gagaaagtga atgccagttg
     3361 ggggatctcc ggcgatggca aaaccgcgtt tattgaaatg gcggcggcca gtgggctgga
     3421 gctggtacct gcggaaaaac gcgatccact cgtgaccact tcacgcggca caggcgagtt
     3481 aatcctgcag gcgctggaga gcggcgcgac aaacattatt atcggcattg gcggtagcgc
     3541 tacaaatgat ggcggcgcag gcatggtaca ggcgctgggg gcgaaattat gcgacgccaa
     3601 cggcaatgaa attggttttg gcggcggtag tcttaatact ctgaatgata ttgatatttc
     3661 cggcctcgat ccgcgcttaa aagattgcgt cattcgcgtc gcttgtgatg tcaccaatcc
     3721 gctggtgggc gataacggcg catcgcgcat ctttggccca caaaagggag ccagtgaagc
     3781 gatgattgtt gagctggaca ataacctctc tcactatgcc gaggtcatta aaaaagcgct
     3841 gcatgttgat gtgaaagatg tccccggcgc aggagctgcg ggtggtatgg gcgcggcgct
     3901 aatggcgttt cttggtgcgg aactgaaaag tggtattgaa atcgtcacta cggcgctgaa
     3961 tctggaggag catattcacg attgtacgct ggtgatcacc ggtgaagggc gtattgacag
     4021 ccagagtatt cacggcaagg taccgattgg cgtcgcaaac gtggcgaaga agtaccataa
     4081 accggtgatt ggcattgcgg gtagcctgac cgatgatgtt ggcgttgtac atcagcatgg
     4141 cattgatgct gtcttcagcg tactgaccag cattggtacg ttggacgaag cattccgcgg
     4201 ggcttatgac aatatctgcc gtgcttcacg taatatcgcc gcgacactgg cgattggaat
     4261 gcgcaacgcg gggtgacaag ggcgcgcaaa ccctctatac tgcgcgccga agctgaccag
     4321 acagtcgccg cttcgtcgtc gtcctcttcg ggggagacgg gcggagggga ggaaagtccg
     4381 ggctccatag ggcagggtgc caggtaacgc ctggggggga aacccacgac cagtgcaaca
     4441 gagagcaaac cgccgatggc ccgcgcaagc gggatcaggt aagggtgaaa gggtgcggta
     4501 agagcgcacc gcgcggctgg taacagtccg tggcacggta aactccaccc ggagcaaggc
     4561 caaatagggg ttcataaggt acggcccgta ctgaacccgg gtaggctgct tgagccagtg
     4621 agcgattgct ggcctagatg aatgactgtc cacgacagaa cccggcttat cggtcagttt
     4681 cacctgattt aacgaaaacc cgccagcgta aaccggcggg tttttgcttt tggaggggca
     4741 gaaagatgaa tgactgtcca cgacgctata cccaaaagaa agcggcttat cggtcagttt
     4801 cacctgatgt acgtaataaa ccgttccggc gggtttcaga ttgttgagtg cgctttattc
     4861 atgccggatg cggcgtgaac gccttatcct gcctacaaaa gcacgcaaat tcaatatatt
     4921 gcagagatgg cgtaggcctg ataagcgtag cgcatcaggc atttttgcgg ttgccatcag
     4981 tttcaaaccc gcgtcagtga gtttttgctt ttggtgcaca gggtaagatg aataaatatc
     5041 t
//
LOCUS       DAZKHF020000286         4991 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2201S31241
            SAMN43267800-rid25106413.denovo.288, whole genome shotgun sequence.
ACCESSION   DAZKHF020000286 DAZKHF020000000
VERSION     DAZKHF020000286.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267800
            Sequence Read Archive: SRR30307263
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4991)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4991)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 4991)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350581.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350581.2
            Genome Coverage       :: 151x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:21:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,652
            CDSs (total)                      :: 5,557
            Genes (coding)                    :: 5,320
            CDSs (with protein)               :: 5,320
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 237
            CDSs (without protein)            :: 237
            Pseudo Genes (ambiguous residues) :: 0 of 237
            Pseudo Genes (frameshifted)       :: 102 of 237
            Pseudo Genes (incomplete)         :: 130 of 237
            Pseudo Genes (internal stop)      :: 52 of 237
            Pseudo Genes (multiple problems)  :: 41 of 237
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4991
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267800-rid25106413.denovo.288"
                     /serotype="O 26:H11"
                     /isolate="2201S31241"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-01"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            <1..169
                     /locus_tag="ACHOSC_RS21240"
                     /old_locus_tag="pgaptmp_004248"
     CDS             <1..169
                     /locus_tag="ACHOSC_RS21240"
                     /old_locus_tag="pgaptmp_004248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000998048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="IS66 family transposase"
                     /protein_id="HGU4550060.1"
                     /translation="HGGERGALLYSLIGTCKLNDVDPESYLRHVPGVIADWPVNRVSE
                     LLPWRIALPAE"
     gene            612..4514
                     /gene="espP"
                     /locus_tag="ACHOSC_RS21245"
                     /old_locus_tag="pgaptmp_004249"
     CDS             612..4514
                     /gene="espP"
                     /locus_tag="ACHOSC_RS21245"
                     /old_locus_tag="pgaptmp_004249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine protease autotransporter EspP"
                     /protein_id="HGU4550061.1"
                     /translation="MNKIYSLKYSHITGGLIAVSELSGRVSSRATGKKKHKRILALCF
                     LGLLQSSYSFASQMDISNFYIRDYMDFAQNKGIFQAGATNIEIVKKDGSTLKLPEVPF
                     PDFSPVANKGSTTSIGGAYSITATHNTKNHHSVATQNWGNSTYKQTDWNTSHPDFAVS
                     RLDKFVVETRGATEGADISLSKQQALERYGVNYKGEKKLIAFRAGSGVVSVKKNGRIT
                     PFNEVSYKPEMLNGSFVHIDDWSGWLILTNNQFDEFNNIASQGDSGSALFVYDNQKKK
                     WVVAGTVWGIYNYANGKNHAAYSKWNQTTIDNLKNKYSYNVDMSGAQVATIENGKLTG
                     TGSDTTDIKNKDLIFTGGGDILLKSSFDNGAGGLVFNDKKTYRVNGDDFTFKGAGVDT
                     RNGSTVEWNIRYDNKDNLHKIGDGTLDVRKTQNTNLKTGEGLVILGAEKTFNNIYITS
                     GDGTVRLNAENALSGGEYNGIFFAKNGGTLDLNGYNQSFNKIAATDSGAVITNTSTKK
                     SILSLNNTADYIYHGNINGNLDVLQHHETKKENRRLILDGGVDTTNDISLRNTQLSMQ
                     GHATEHAIYRDGAFSCSLPAPMRFLCGSDYVAGMQNTEADAVKQNGNAYKTNNAVSDL
                     SQPDWETGTFRFGTLHLENSDFSVGRNANVIGDIQASKSNITIGDTTAYIDLHAGKNI
                     TGDGFGFRQNIVRGNSQGETLFTGGITAEDSTIVIKDKAKALFSNYVYLLNTKATIEN
                     GADVTTQSGMFSTSDISISGNLSMTGNPDKDNKFEPSIYLNDASYLLTDDSARLVAKN
                     KASVVGDIHSTKSASIMFGHDESDLSQLSDRTSKGLALGLLGGFDVSYRGSVNAPSAS
                     ATMNNTWWQLTGDSALKTLKSTNSMVYFTDSANNKKFHTLTVDELATSNSAYAMRTNL
                     SESDKLEVKKHLSGENNILLVDFLQKPTPEKQLNIELVSAPKDTNENVFKASKQTIGF
                     SDVTPVITTRETDDKITWSLTGYNTVANKEATRNAAALFSVDYKAFLNEVNNLNKRMG
                     DLRDINGEAGAWARIMSGTGSASGGFSDNYTHVQVGVDKKHELDGLDLFTGFTVTHTD
                     SSASADVFSGKTKSVGAGLYASAMFDSGAYIDLIGKYVHHDNEYTATFAGLGTRDYST
                     HSWYAGAEAGYRYHVTEDAWIEPQAELVYGSVSGKQFAWKDQGMHLSMKDKDYNPLIG
                     RTGVDVGKSFSGKDWKVTARAGLGYQFDLLANGETVLRDASGEKRIKGEKDSRMLMSV
                     GLNAEIRDNVRFGLEFEKSAFGKYNVDNAVNANFRYSF"
     gene            complement(4762..>4991)
                     /locus_tag="ACHOSC_RS21250"
                     /old_locus_tag="pgaptmp_004250"
     CDS             complement(4762..>4991)
                     /locus_tag="ACHOSC_RS21250"
                     /old_locus_tag="pgaptmp_004250"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4550062.1"
                     /translation="AQNQPAETVRAAAAATGRFAVAFPVRVAPGSGCFMKTNAPRLFF
                     VDKEAGVSFDVFHVLSVSFPVTACATHDGGM"
BASE COUNT         1515 a         1133 c         1113 g         1230 t
ORIGIN      
        1 ccatggtggt gagcggggag cgctactgta cagcctgatc gggacgtgca aactgaatga
       61 cgtggatcca gaaagctacc ttcgccatgt gcctggcgtc atagcagact ggccggtcaa
      121 ccgggtcagc gaactgcttc cgtggcgcat agcactgcca gctgaataac acatccccgt
      181 caatacggcc ctcgctgtac gcttacgcat aaatattgaa tacatttact gagcatgcaa
      241 aagaaaaaga caaactaatc ccggcctttc acagaatttc ctctatcaga ggctgagttg
      301 acaacctgct cacccaggaa atagtaacac cagaaaacat gaagagactc tgataaacaa
      361 tctcgataca tattcctcat ctttcacagc atctcacttc tttaacagga attattatta
      421 tctacgcctc ctcctttcat gtatccctca tgaatgatta agataaacat actgtgtttg
      481 ttatccgtat gataacaaac acataaagga gtaaacattt atctggatga tgattacaat
      541 gttcacgact gtcagagaca cacctctgat acattagaaa aaccaatgtt tcccttaaaa
      601 atggagctta tatgaataaa atatactctc ttaaatacag ccatattaca ggagggttaa
      661 tcgctgtttc tgaattatcc ggcagagtat catcaagagc aactggtaag aaaaaacaca
      721 aacgcatact tgcattatgt tttttaggct tattacaatc ctcatattct tttgcgtcac
      781 agatggatat ttcaaatttc tacatccgtg actatatgga ttttgcacag aacaagggca
      841 tatttcaggc tggcgcaaca aatattgaaa tagtgaagaa agatggctcc accctgaaac
      901 taccggaagt accatttcct gacttctcac cggttgcaaa caaagggtca accacatcta
      961 ttggtggtgc atacagtatc acagccacac acaatacgaa aaaccaccac tcagttgcga
     1021 cgcaaaactg ggggaacagc acgtacaaac aaactgactg gaatacttca catcctgatt
     1081 ttgcagtatc ccgacttgac aagtttgttg ttgagacccg aggtgcgact gaaggcgcag
     1141 atatttcgtt atcaaaacag caggcacttg aacgttacgg ggttaattat aaaggagaaa
     1201 agaaacttat cgcattcaga gccggctctg gtgtggtatc cgttaaaaaa aatggacgca
     1261 taactccatt taatgaggtt tcttataagc cagaaatgtt aaatggctct ttcgttcaca
     1321 ttgatgactg gagtggatgg ctgatattaa ccaacaacca gtttgatgag tttaataaca
     1381 ttgcctctca gggtgacagc ggttcagcac tgttcgtcta tgataaccaa aagaaaaagt
     1441 gggttgtcgc tggaactgtc tgggggattt ataattacgc caatggcaaa aaccacgcag
     1501 catacagtaa atggaaccag acaaccattg acaacctgaa gaacaagtat tcttacaacg
     1561 tggatatgtc aggagctcag gttgcaacca ttgaaaatgg aaaactgaca ggcactggct
     1621 cagacaccac cgatataaaa aataaggact taatatttac tggcggtgga gatatcctcc
     1681 tgaaatcctc ttttgataat ggtgctggcg gtcttgtctt taatgataaa aagacctatc
     1741 gagtaaacgg ggatgatttc acctttaaag gtgccggtgt tgatacaaga aacggcagca
     1801 ccgttgagtg gaatatccgg tatgataata aagacaacct tcacaaaatt ggtgatggca
     1861 cattagatgt ccgaaaaacc cagaacacca acctgaaaac aggtgagggt cttgtcattc
     1921 ttggagctga aaaaacattc aataatatct acataaccag tggtgatgga actgtccgac
     1981 tgaatgcaga aaatgcactg tctggcggtg aatacaacgg tattttcttt gcgaaaaatg
     2041 gcggaactct tgacctgaac ggatataatc agtctttcaa taaaattgct gcaactgatt
     2101 caggtgctgt aataaccaat acgtcaacca aaaaatccat tttatccctg aataatactg
     2161 ctgactatat ctatcacggc aacataaacg ggaatctgga cgtacttcag catcatgaga
     2221 cgaaaaaaga gaaccgtcgt cttattcttg atgggggcgt ggacacaaca aatgatataa
     2281 gcctgcgtaa tacacaactg tccatgcagg gacatgccac tgaacatgcc atttatcggg
     2341 atggagcttt ctcttgttca ctaccagctc ctatgcgctt tttgtgtggc agtgattatg
     2401 ttgcaggaat gcaaaataca gaagctgatg ctgtaaaaca aaacggaaat gcctataaaa
     2461 ccaacaatgc tgtctctgat ttatcgcagc cagactggga aaccggaaca ttcagatttg
     2521 gaacgctaca tcttgaaaat tccgattttt ctgttggtcg taatgcaaat gtaatcgggg
     2581 acattcaggc cagtaaatca aacattacta ttggtgacac tacagcatat attgatttgc
     2641 atgctggtaa aaatattacc ggtgatggtt ttggcttccg ccagaatatt gtgcgtggaa
     2701 actcacaagg agaaacgctg tttacaggag ggatcacagc agaagacagc actatcgtta
     2761 ttaaagataa agcaaaagca ttattttcaa attatgtata cctgctgaac acaaaagcaa
     2821 ccatagagaa cggtgctgat gtgacaactc aaagtggtat gttctccacg agcgatatca
     2881 gcatctctgg taatctgtcc atgacaggca atcccgacaa agacaataaa ttcgagccct
     2941 caatatatct gaatgatgct tcttatctac tgactgacga ctccgccaga ctcgttgcca
     3001 aaaataaagc atctgtggtg ggagatatac actccactaa aagtgcatcc atcatgtttg
     3061 gtcatgatga aagcgacctc tcgcagttgt ctgacagaac ctcaaaaggg cttgcacttg
     3121 gtcttttagg tggctttgat gtctcatatc gcggttcagt caatgccccg tcagcatctg
     3181 ccactatgaa caatacctgg tggcaactaa ccggagattc tgcgctgaaa acactgaaaa
     3241 gtacaaacag catggtctat ttcactgaca gcgcaaacaa taagaaattc catacgctga
     3301 cggtcgatga gctggcaacc agcaacagcg cctatgcgat gcgtacaaac ctttctgaat
     3361 cagacaaact ggaggtcaaa aaacacctgt ctggtgagaa caatatttta ctcgttgatt
     3421 tccttcagaa accaacgcct gaaaaacaac tgaatattga actggtaagc gcgccaaaag
     3481 acaccaatga aaatgtcttt aaagccagta aacaaaccat tggtttcagt gatgtaacgc
     3541 cggtcattac aaccagggaa accgatgaca aaataacatg gtcactgaca ggctataaca
     3601 cggtagcaaa caaggaagca acccggaatg ccgccgccct gttctctgtt gactataaag
     3661 cgtttctgaa cgaggtcaac aacctgaaca aacgtatggg tgacctgcgt gatatcaacg
     3721 gcgaagccgg tgcatgggca cgcatcatga gcggtaccgg ctctgccagt ggtggtttca
     3781 gtgacaacta cacgcacgtt caggtcgggg tcgacaaaaa acacgagctg gacggactgg
     3841 atttgtttac cggtttcact gtcacacaca ctgacagcag tgcctccgcc gatgttttca
     3901 gtggtaaaac gaagtctgtg ggggctggcc tgtatgcttc cgccatgttt gattccggtg
     3961 cctatatcga cctgattggc aagtatgttc accatgataa tgagtacact gcaacctttg
     4021 ccggactcgg aacccgtgat tacagcacgc attcatggta tgccggtgca gaagcgggct
     4081 accgctatca tgtcactgag gatgcctgga ttgagccaca ggctgagctg gtttacggtt
     4141 ctgtatccgg taaacagttt gcatggaagg accagggaat gcatctgtcc atgaaggaca
     4201 aggactacaa tccgctgatt ggccgaacgg gtgtggatgt gggtaaatcc ttctctggta
     4261 aggactggaa agtgacagcc cgtgccggtc tgggctacca gttcgacctg ctggctaacg
     4321 gcgaaaccgt attgcgggat gcatctggtg aaaaacgcat caaaggtgaa aaggacagcc
     4381 gtatgctgat gtccgttggc ctgaatgcag aaatcaggga taacgtccgc tttggactgg
     4441 agtttgagaa atccgccttt ggtaagtaca acgttgataa tgcagtcaac gctaatttcc
     4501 gttactcgtt ctgatactcc gtattcaaaa gggcctgcgg gccctttacg ctcagtgcct
     4561 gccgccctga tatgtctgag agacatttcc gggatgtcag tcgactggtt tacttaactg
     4621 cgatgactaa agctggcagt aatgccggct tttttacggc cacatacgtc ttcctctcat
     4681 ccggaactgt gatacaaatc ctgcatacag gattgaccgc cggttcctgc aaatggcaca
     4741 ttttcagtaa gatagcgaag gttacattcc cccgtcatgt gtggcacaag ctgtcaccgg
     4801 gaaagaaacg gaaagcacat gaaaaacatc aaaactcaca ccggcctcct tatccacaaa
     4861 gaacaaacgc ggcgcgttcg tcttcatgaa acacccactg cctggtgcca cacgcaccgg
     4921 gaatgctaca gcaaaacgac cggtcgccgc tgcggcagcc ctgacagtct cagcaggctg
     4981 attctgagca g
//
LOCUS       DAZKHF020000287         4920 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2201S31241
            SAMN43267800-rid25106413.denovo.289, whole genome shotgun sequence.
ACCESSION   DAZKHF020000287 DAZKHF020000000
VERSION     DAZKHF020000287.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267800
            Sequence Read Archive: SRR30307263
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4920)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4920)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 4920)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350581.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350581.2
            Genome Coverage       :: 151x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:21:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,652
            CDSs (total)                      :: 5,557
            Genes (coding)                    :: 5,320
            CDSs (with protein)               :: 5,320
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 237
            CDSs (without protein)            :: 237
            Pseudo Genes (ambiguous residues) :: 0 of 237
            Pseudo Genes (frameshifted)       :: 102 of 237
            Pseudo Genes (incomplete)         :: 130 of 237
            Pseudo Genes (internal stop)      :: 52 of 237
            Pseudo Genes (multiple problems)  :: 41 of 237
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4920
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267800-rid25106413.denovo.289"
                     /serotype="O 26:H11"
                     /isolate="2201S31241"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-01"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            complement(<1..358)
                     /locus_tag="ACHOSC_RS21255"
                     /old_locus_tag="pgaptmp_004251"
     CDS             complement(<1..358)
                     /locus_tag="ACHOSC_RS21255"
                     /old_locus_tag="pgaptmp_004251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000176498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OB-fold-containig protein"
                     /protein_id="HGU4550063.1"
                     /translation="MTLTLLTAYNVPYLAALTFVLLTGIAELIALLCGHSLSSAMDTP
                     DLPEGLTGEALDWLNIGRIPLLIVLCMLAGFFGISGILLQGLIIHLLQAPAPNILLAP
                     LCLLLTCPLVHRTGRLI"
     gene            complement(625..1290)
                     /locus_tag="ACHOSC_RS21260"
                     /old_locus_tag="pgaptmp_004252"
     CDS             complement(625..1290)
                     /locus_tag="ACHOSC_RS21260"
                     /old_locus_tag="pgaptmp_004252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000154135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="traC"
                     /protein_id="HGU4550064.1"
                     /translation="MTILSLENPVILLLENMLEALSTAPDSINNERRRRRYLLNWLES
                     ARQMSAFRGMAEEFTTLRKLLATNTDIPVANTLKSLLESGNNAALYDLYRFRSALSAC
                     RSLGWQIGTCAWNDQLLSETLSRAQTGKRHILQLNSTRDTFSTTGRMLRPVAFSLIHP
                     ASLESSEVEEIFRDEGFILAHGRECSLNGSGRNMLSRILHVGHSGLPKAEWGIDHSNH
                     LLH"
     gene            complement(1431..2072)
                     /locus_tag="ACHOSC_RS21265"
                     /old_locus_tag="pgaptmp_004253"
     CDS             complement(1431..2072)
                     /locus_tag="ACHOSC_RS21265"
                     /old_locus_tag="pgaptmp_004253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000335849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006354 - DNA-templated transcription
                     elongation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0140673 - transcription elongation-coupled
                     chromatin remodeling [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination NusG
                     family protein"
                     /protein_id="HGU4550065.1"
                     /translation="MCLSELQAGLNEDQPLFPTHALCIRRSQQHRSPSAGIDFRGAFR
                     ILNIRELHQRNWYLAQYIPAGKNREHLFSWLSEQHVLPWTPLILKKVRRTDKVCGYRR
                     HIHAVFPGYFFLKADPERHSFTHLRRHSAFLNFVKMAGEIKTVREDIVQSLMKVYPDP
                     ALNPAAREELDAASTLWLTKARYQYLLRLDAPPLPESRIALLLHLVSDDGALT"
     gene            2774..3805
                     /gene="repA"
                     /locus_tag="ACHOSC_RS21270"
                     /old_locus_tag="pgaptmp_004254"
     CDS             2774..3805
                     /gene="repA"
                     /locus_tag="ACHOSC_RS21270"
                     /old_locus_tag="pgaptmp_004254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000907860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006276 - plasmid maintenance [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid replication initiator RepA"
                     /protein_id="HGU4550066.1"
                     /translation="MAGLKNTPYNAVHWSQLAPEEQIRFWEDYEAGRATSFLVEPERK
                     RTRRRRGEHSTKPKCENPSWYRPARYKELSGQLGHAYNRLVKKAPVTGEQSLRMRMSR
                     HPFYVQKRTFVGRKYAFRPEKQRLLDAIWPVLVSFSDAGTHTVGMSVTRLAKEISPKD
                     SKGNVIPELEVTVSRLSRLLAEQVRFGVLGVSEETMWDRETRQRLPRYVWITPAGWQM
                     LGVDMVKLHEQQQKRLRESEIRQQLIREGVLREDEDISVHAARKRWYLQRSQDALKHR
                     RAKAAASKRARRLKKLPADQQIHEMAEYLRKRLPPDEAYFCSDDHLKRLAIRELRQLE
                     LTLAAPPPH"
BASE COUNT         1318 a         1206 c         1303 g         1093 t
ORIGIN      
        1 caatcagtct gccggtgcgg tgtacgagag gacatgtcag cagcaggcaa agtggtgcaa
       61 ggagaatgtt cggggcagga gcctgcagca gatgaataat aagaccctgc aggagaatgc
      121 cggatatacc aaaaaaacct gccagcatac agaggacgat cagcaggggg atccgtccga
      181 tgttcagcca gtccagcgcc tctcctgtca gcccctccgg taaatcaggt gtatccatag
      241 ctgaagagag ggagtgtccg cagagcaggg cgataagttc agcgatacca gtaagcagaa
      301 caaaagtcag ggctgccaga tagggtacgt tatacgcggt aagtaacgtc agtgtcattt
      361 ttcctcctgt ggcaggtcag tcaaacaaca ttatggcgtg aagcttccgc ttctttaatt
      421 gtcagaagaa gcatatcgtc agtcatcaga agacaagggg atgtctgcag gaggggatga
      481 taacaactga agcctcacgc cggaatgcgc tttatgacaa ccggagagga cggataataa
      541 aaaatgggac atccacatca gcaaaccgcg atttgcgaaa aaaagtcagc ggagagtggc
      601 tgccgttgtg gctgtcccgg tatttcagtg taacaggtga ttgctgtgat ctatgcccca
      661 ttcggctttc ggtaaacctg aatgccccac atgaagaatg cgtgacagca tgtttctgcc
      721 gctgccgttc aggctgcatt cgcgtccgtg cgcgagtata aaaccttcat cccggaatat
      781 ctcttccact tctgaagatt cgagggatgc cggatgaatt aatgaaaatg ccaccggtct
      841 gagcattctg cccgtggtgg aaaaggtgtc tctggtactg ttcagctgca ggatatgacg
      901 tttacctgtt tgcgcacggg aaagtgtctc actcagcagc tggtcgttcc atgcgcacgt
      961 gccaatctgc catcccagac ttctgcacgc ggaaagagcc gagcggaagc ggtacaggtc
     1021 gtataatgct gcgttattgc cactttccag cagtgatttc agcgtatttg ctaccggtat
     1081 gtccgtattg gtggccagca gttttcgcag agtcgtaaac tcttctgcca ttcctctgaa
     1141 tgcgctcatc tgacgcgcag attcaagcca gttcagcagg taacgccggc gacgccgttc
     1201 gttgttgata ctgtccgggg cggtggagag tgcttcgagc atattctcca gtaacagaat
     1261 gacagggttt tccagggata gaatggtcat aaaggcgtcc tgaaaaaatg cccgacagcc
     1321 gctctccgta caccgtaagg tgtcaccggt gccgggcatt gtggaaacag aaggaaaaaa
     1381 aatcccggcc ataaaggccg ggaaaagtcg tcaaattaag acagcaattt tcatgtcaac
     1441 gctccgtcat cagataccag gtgcaggagc agggcgatcc tcgactccgg cagaggcggt
     1501 gcatcaagcc ggaggaggta ctggtaacgc gcttttgtca gccagagggt ggaggcagca
     1561 tccagctcct ccctggctgc cggattgagc gcaggatccg ggtacacttt catcaaagac
     1621 tgaacgatat cctccctgac ggttttgatt tcaccggcca ttttcacgaa gttcagaaag
     1681 gcactgtgac ggcgaaggtg agtgaagctg tggcgttcgg ggtctgcttt caggaaaaag
     1741 taaccgggaa acacggcgtg tatatgtcga cggtaaccgc agactttatc cgtgcggcgg
     1801 acttttttca gtatcagggg tgtccaggga aggacgtgtt gttcggaaag ccaggaaaag
     1861 agatgttcgc ggtttttgcc ggcagggatg tactgagcca gataccagtt gcgttgatgt
     1921 aattctctga tattcaaaat ccggaacgct ccgcgaaaat cgatccccgc agaggggctt
     1981 cgatgttgtt gtgaacggcg aatacataaa gcatgtgtcg gaaacagcgg ctgatcctcg
     2041 ttcagcccgg cctgcagttc tgacaggcac atgatttacg cactcttgta agccttgtgg
     2101 acggtttcga ggccggcgaa aaccgacaaa acctgcaaac aggattaatg gctggcaaac
     2161 atattacata aggatatatc tgtggcaaga gcgaagataa gtagttaaat agatcgttat
     2221 atttgataag gcagcatatt acgctgggtt gtgtgaggtg tttggatgat tagatcgtag
     2281 atcgtaatca caagatcgat tcatccaaaa aacatgaaaa acacagcata aaagatcgtt
     2341 tgtggaattg tctatttttt gacacagttc aaaaaaacga cgttttttag gtggttaatg
     2401 agaggcctgg taaatcagag aaatcgtgtt ggcttttcaa agcggtggaa aaggggtata
     2461 ttgcggatcg ttatgcagtg gcttttggga tcgtcaggat ccgggaagtc agaaaatggc
     2521 aggatgcgcc ataaggcatt caggacgtat ggcagaaacg acggcagttt gccggtgccg
     2581 gaaggctgaa aaaagtttca gaaggccata aaaggaaaac ccccataatc ttctcatcga
     2641 acttggcgga acgttagaag attatggggg cctcacagaa tacgggataa gtatatatga
     2701 aaccgtgtca gagattcaac ccttcgcagt gtattaacgc acggcattct gtttccgcca
     2761 taagcgacag cttgtggcag gtctgaagaa tactccatat aacgcagtac actggagtca
     2821 gttagcaccc gaagagcaga tccgtttctg ggaagactat gaagcgggaa gggcgacctc
     2881 tttcctggtt gaaccggaaa ggaagcgcac caggcgccgt cgtggcgaac actccactaa
     2941 acccaaatgc gaaaatccgt cctggtatcg tcctgcgcgc tataaggagc tgagcgggca
     3001 gctcgggcac gcctacaacc gtctggtgaa aaaggccccg gtgaccggcg agcagagcct
     3061 gcgtatgcgt atgtcgcggc atccttttta cgtgcagaaa cggacgttcg tcggtcgcaa
     3121 atatgctttc cggccggaaa aacaacgcct cctcgatgcc atctggccgg ttctggtcag
     3181 cttcagtgat gccggcacac acactgtggg gatgagtgtc actcgcctgg ctaaagaaat
     3241 cagtccgaaa gacagtaagg gaaatgttat tccggaactg gaagtgacgg tctcccgtct
     3301 ttcccgtctg ctggccgaac aggtgcgttt tggtgtgctg ggtgtttcag aggaaacgat
     3361 gtgggaccgt gaaacccgcc agcgtctgcc gcgctatgtc tggataacac cggctggctg
     3421 gcagatgctg ggcgtcgaca tggtaaaact tcacgaacag cagcagaaac gactgcgtga
     3481 aagtgaaatc cgccagcagc tcattcggga aggtgttctg cgtgaggatg aagatatctc
     3541 cgtacatgcg gccagaaaac gctggtatct gcagcgcagt caggatgcgc tgaaacaccg
     3601 tcgtgcgaaa gcagcagcca gtaagcgcgc cagacgcctg aagaaactgc ctgccgacca
     3661 gcagattcat gagatggcag agtatctcag gaagcgtctg ccgccggatg aagcctattt
     3721 ttgttccgat gaccatctga agcgactggc catcagggag ttgcgtcagc ttgaactgac
     3781 gctggctgcc ccgccaccgc actagacagc accattccct cagcactgaa tcatcgccag
     3841 cccctccggg gctttcggcg caggtatcgc tcagcccaaa atccgcagta atcaccttaa
     3901 atcccctcag aggggcatat ttgctcataa aaccacgcat cagtcatcag aacgtggcca
     3961 cgttgtttca gttatccaca taaatccgca aacaaagaac tttaagaagc tgcaaacctg
     4021 aaacagcaaa cctgcaatat agtcttaacc ccattattta atcccctgcg ttgcttcgcc
     4081 gcagcgaaag tctttatctc tgaaaccact gtgaacaaac acaaagaggc cttcgcttgc
     4141 agcggccaag gccgcgccgc tcagaatcta aaagcacctc ccacgctgac gcgcgggccc
     4201 cgaacctcac cgttctggaa ccacaacaaa aaaacatcag gaataaaaac accacacaaa
     4261 cgcagcaccg tgcccacccc tcataactga aaagcgccgc cgccccggcc caaagggccg
     4321 gaacagagtc gcttttaatt atgaatgttg taactaaaca tcatcatcgc tttaagaccc
     4381 catttgtcta gcagggcgat ggcccactgc gtgaaccatc accctgcaca cagggtattt
     4441 tatgtgcaat tgtcaaccac tgaatcggaa ccccagaggg gcccccacga ttcagaagtt
     4501 gacaatttaa gcacagggac tacatgagaa aaatcgtggt tcgtctgtca tacacgcgcg
     4561 taagcggcac tgataaccct cttacgcgga aatacgccca taagcgctaa cttaagggtt
     4621 gaaccgacgc cacggtgtct cgttaagact tcaattgcgt tttgtaggca gtcagggata
     4681 ctgtcccatg aatggccacc tgtaagctcc agataaccat ttttgttatt ctccacaacg
     4741 aattccgcat accggaaatc agcgctactt ctggggagga atggccttaa gagtgataaa
     4801 gattatatgc ttttcataga gagactcatc tatgccggta acactgaaaa atgtattgtg
     4861 ttccaagaag tttttttgag tgaacatatt ttcgctcaca acaatcgaat tattcaaagc
//
LOCUS       DAZKHF020000288         4920 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2201S31241
            SAMN43267800-rid25106413.denovo.290, whole genome shotgun sequence.
ACCESSION   DAZKHF020000288 DAZKHF020000000
VERSION     DAZKHF020000288.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267800
            Sequence Read Archive: SRR30307263
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4920)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4920)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 4920)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350581.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350581.2
            Genome Coverage       :: 151x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:21:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,652
            CDSs (total)                      :: 5,557
            Genes (coding)                    :: 5,320
            CDSs (with protein)               :: 5,320
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 237
            CDSs (without protein)            :: 237
            Pseudo Genes (ambiguous residues) :: 0 of 237
            Pseudo Genes (frameshifted)       :: 102 of 237
            Pseudo Genes (incomplete)         :: 130 of 237
            Pseudo Genes (internal stop)      :: 52 of 237
            Pseudo Genes (multiple problems)  :: 41 of 237
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4920
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267800-rid25106413.denovo.290"
                     /serotype="O 26:H11"
                     /isolate="2201S31241"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-01"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            <1..860
                     /locus_tag="ACHOSC_RS21275"
                     /old_locus_tag="pgaptmp_004255"
     CDS             <1..860
                     /locus_tag="ACHOSC_RS21275"
                     /old_locus_tag="pgaptmp_004255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HGU4550067.1"
                     /translation="RYSDILNYRNELLHGETLYLDNPRSNKKGRTVRTVDNYIALLCS
                     LLRFAYQSGFISTKPFEGVKKLQRNRIKPDPLSKTEFNALMESEKGQSQNMWKFAVYS
                     GLRHGELAALAWEDVDLEKGIVNVRRNLTILDMFGPPKTNAGIRTVTLLQPALEALKE
                     QYKLTGHHRKSEITFYHREYGRTEKQKLHFVFMPRVCNGKQKPYYSVSSLGARWNAAV
                     KRAGIRRRNPYHTRHTFACWLLTAGANPAFIASQMGHETAQMVYEIYGMWIDDMNDEQ
                     IAMLNARLS"
     gene            912..1847
                     /gene="ttcA"
                     /locus_tag="ACHOSC_RS21280"
                     /old_locus_tag="pgaptmp_004256"
     CDS             912..1847
                     /gene="ttcA"
                     /locus_tag="ACHOSC_RS21280"
                     /old_locus_tag="pgaptmp_004256"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiocytidine(32) synthetase TtcA"
                     /protein_id="HGU4550068.1"
                     /translation="MQENQQITKKEQYNLNKLQKRLRRNVGEAIADFNMIEEGDRIMV
                     CLSGGKDSYTMLEILRNLQQSAPINFSLVAVNLDQKQPGFPEHVLPEYLEKLGVEYKI
                     VEENTYGIVKEKIPEGKTTCSLCSRLRRGILYRTATELGATKIALGHHRDDILQTLFL
                     NMFYGGKMKGMPPKLMSDDGKHIVIRPLAYCREKDIQRFADAKAFPIIPCNLCGSQPN
                     LQRQVIADMLRDWDKRYPGRIETMFSAMQNVVPSHLCDTNLFDFKGITHGSEVVNGGD
                     LAFDREEIPLQPAGWQPEEDENQLDELRLNVVEVK"
     gene            complement(1976..3349)
                     /gene="dbpA"
                     /locus_tag="ACHOSC_RS21285"
                     /old_locus_tag="pgaptmp_004257"
     CDS             complement(1976..3349)
                     /gene="dbpA"
                     /locus_tag="ACHOSC_RS21285"
                     /old_locus_tag="pgaptmp_004257"
                     /EC_number="3.6.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003724 - RNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RNA helicase DbpA"
                     /protein_id="HGU4550069.1"
                     /translation="MTAFSTLNVLPPAQLTNLNELGYLTMTPVQAAALPAILAGKDVR
                     VQAKTGSGKTAAFGLGLLQQIDASLFQTQALVLCPTRELADQVAGELRRLARFLPNTK
                     ILTLCGGQPFGMQRDSLQHAPHIIVATPGRLLDHLQKGTVSLDALNTLVMDEADRMLD
                     MGFSDAIDDVIRFAPASRQTLLFSATWPEAIAAISGRVQRDPLAIEIDSTDALPPIEQ
                     QFYETSSKGKIPLLQRLLSLHQPSSCVVFCNTKKDCQAVCDALNEVGQSALSLHGDLE
                     QRDRDQTLVRFANGSARVLVATDVAARGLDIKSLELVVNFELAWDPEVHVHRIGRTAR
                     AGNSGLAISFCAPEEAQRANIISDMLQIKLNWQTPPANSSIVPLEAEMATLCIDGGKK
                     AKMRPGDVLGALTGDIGLDGADIGKIAVHPAHVYVAVRQAVAHKAWKQLQGGKIKGKT
                     CRVRLLK"
     gene            complement(3379..3552)
                     /gene="ynaL"
                     /locus_tag="ACHOSC_RS21290"
                     /old_locus_tag="pgaptmp_004258"
     CDS             complement(3379..3552)
                     /gene="ynaL"
                     /locus_tag="ACHOSC_RS21290"
                     /old_locus_tag="pgaptmp_004258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YnaL"
                     /protein_id="HGU4550070.1"
                     /translation="MTTLIYLQIPVPEPIPSDPVPVPDPIPRPQPMPDPPPDEEPIKL
                     SHRERRSARIRAC"
     gene            complement(3827..4810)
                     /gene="zntB"
                     /locus_tag="ACHOSC_RS21295"
                     /old_locus_tag="pgaptmp_004259"
     CDS             complement(3827..4810)
                     /gene="zntB"
                     /locus_tag="ACHOSC_RS21295"
                     /old_locus_tag="pgaptmp_004259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005385 - zinc ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0071577 - zinc ion transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="zinc transporter ZntB"
                     /protein_id="HGU4550071.1"
                     /translation="MEAIKGSDVNVPDAVFAWMLDGRGGVKPLENTDVIDEAHPCWLH
                     LNYVHHDSAQWLATTPLLPNNVRDALAGESTRPRVSRLGEGTLITLRCINGSTDERPD
                     QLVAMRVYMDGRLIVSTRQRKVLALDDVVSDLEEGTGPTDCGGWLVDVCDALTDHSSE
                     FIEQLHDKIIDLEDNLLDQQIPPRGFLALLRKQLIVMRRYMAPQRDVYARLASERLPW
                     MSDDQRRRMQDIADRLGRGLDEIDACIARTGVMADEIAQVMQENLARRTYTMSLMAMV
                     FLPSTFLTGLFGVNLGGIPGGGWQFGFSIFCILLVVLIGGVALWLHRSKWL"
     gene            complement(4824..4871)
                     /gene="ydaN"
                     /locus_tag="ACHOSC_RS21300"
                     /old_locus_tag="pgaptmp_004260"
     CDS             complement(4824..4871)
                     /gene="ydaN"
                     /locus_tag="ACHOSC_RS21300"
                     /old_locus_tag="pgaptmp_004260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YnaN"
                     /protein_id="HGU4550072.1"
                     /translation="MLRNRARRLDYKIMR"
BASE COUNT         1339 a         1238 c         1190 g         1153 t
ORIGIN      
        1 ttcgttatag cgatatctta aactaccgga atgaactgct gcatggagaa acgctttacc
       61 tggataatcc aagatccaac aaaaaaggaa gaaccgtgcg cacagttgat aactatatcg
      121 ccctgctctg ttcgctgtta cgttttgcgt atcagtcggg atttatatca accaaaccat
      181 ttgaaggagt aaaaaaatta cagcgaaaca gaataaagcc tgatccgtta tctaaaacag
      241 aattcaatgc attaatggaa agtgaaaaag gacagagcca gaacatgtgg aaatttgcag
      301 tttactcagg acttcgtcac ggggaactgg cagctctggc gtgggaggat gtggatctcg
      361 aaaagggaat agtgaatgtc agaagaaacc tgacgatact tgatatgttc ggtcccccaa
      421 aaacaaatgc cgggatccgg acagtaacac tactgcagcc tgctcttgaa gcactgaagg
      481 agcaatacaa actgaccggg catcatcgca aaagcgaaat caccttttat catcgggagt
      541 acggcagaac cgaaaagcaa aaactgcatt ttgttttcat gcccagggtg tgtaacggaa
      601 aacaaaaacc ttattactcg gtaagcagtt tgggggcaag gtggaatgca gcagtaaaac
      661 gtgctggtat tcgccgccgt aatccgtacc atacgcggca tacttttgcc tgctggctgt
      721 tgacggcagg agcgaacccg gcatttatag ccagccaaat ggggcatgaa actgcgcaga
      781 tggtgtatga aatttacggt atgtggattg atgacatgaa cgacgaacag atagccatgt
      841 tgaatgcgcg gttatcatag ttgcaaagtt tgcccccaat ttgccccatt tagtaccaga
      901 gaactgaaat aatgcaagaa aatcaacaaa ttacaaagaa agaacaatac aacctgaaca
      961 aattacaaaa acgtctgcgt cgtaacgtgg gcgaagccat tgctgacttc aatatgattg
     1021 aagaaggcga tcgcatcatg gtttgcctct ccgggggtaa agacagctat accatgctgg
     1081 agattctgcg caatttgcag caaagcgcgc caatcaattt ttcgctggtg gctgttaacc
     1141 tcgatcaaaa gcaaccgggc ttcccggaac acgttctgcc cgagtatctt gaaaagctgg
     1201 gcgttgagta caagattgtt gaagagaata cttacggtat cgtgaaagag aagattccag
     1261 agggcaaaac cacttgctca ctgtgttctc gccttcgtcg cggtatcctt tatcgtaccg
     1321 caacggaact gggggcgacg aagatcgcgt tgggtcacca tcgtgacgat atcctgcaaa
     1381 cgttgttctt aaatatgttc tacggcggca agatgaaagg tatgcctccg aaactgatga
     1441 gcgatgatgg caaacatatc gttattcgtc cgctggccta ctgccgcgag aaagatattc
     1501 agcgatttgc cgatgcaaaa gcgttcccga ttattccgtg caacctgtgc ggttcacagc
     1561 ctaacctgca acgtcaggtg attgctgaca tgttgcgtga ctgggataaa cgttatccag
     1621 ggcgtatcga gacgatgttc agcgcgatgc agaatgtggt gccgtcgcat ctgtgcgata
     1681 ccaacctgtt cgattttaaa ggcattaccc acggttctga agtggttaac gggggtgatc
     1741 tggcgtttga tcgcgaagag atcccactac aaccggcggg ctggcagcca gaagaagatg
     1801 aaaatcagtt ggatgagtta cgactgaatg tggttgaagt gaaataacca ggatagcgcc
     1861 cgatgcgcaa gcgtatcggg ctactcttat ggaggccgga taagacgcgg ccagcgtcgc
     1921 atccggcaat cccgaataag atgtttactc ttgcacccgg caattcaaca tttcattatt
     1981 ttaataaccg cacccggcac gtttttcctt taatcttccc gccctgtaac tgtttccatg
     2041 ctttatgagc aacagcctga cggaccgcga catagacatg cgccggatgc acggcgattt
     2101 tgccaatatc tgcgccatca agcccgatat ctcctgtcag tgcacctaat acatcacccg
     2161 ggcgcatttt ggcttttttc ccgccatcga tacacaacgt tgccatttct gcttccagcg
     2221 gcacaatgga actattagct ggcggcgttt gccagttaag ttttatctgc aacatgtcag
     2281 aaatgatatt ggcccgctgt gcttcttccg gagcacagaa actgatcgcc agaccgctat
     2341 ttcctgcacg agctgtacga ccgatgcgat gtacatgaac ttcagggtcc cacgccagct
     2401 caaagttcac caccagctca agcgatttaa tatccagacc acgcgcagca acatcagtag
     2461 caaccagtac acgggcgcta ccgttagcaa aacgtaccag agtctgatcg cgatcgcgtt
     2521 gctccagatc gccgtgtaac gacaatgcac tttgccctac ttcattcagc gcgtcgcaga
     2581 cagcctggca atcttttttg gtattgcaaa acaccacgca agaggatggc tgatgcaagc
     2641 ttaataaccg ttgcaacaga gggattttgc ctttgctgga tgtctcataa aattgttgtt
     2701 caatgggtgg caaagcatct gttgagtcaa tttcaatcgc caaaggatcg cgttgtactc
     2761 gtccgctgat tgcggcgatg gcttccggcc aggttgccga aaacagaagc gtctgtcgag
     2821 atgcaggcgc aaaacggatg acatcatcaa tggcatcgct aaatcccata tccagcatgc
     2881 ggtcggcctc atccatcacc aaagtattca gcgcatccag tgataccgtg cctttttgca
     2941 agtgatccag caaacgaccc ggcgttgcca cgataatatg cggcgcatgt tgcaacgaat
     3001 cacgctgcat accgaacggt tgaccaccgc acaacgtcaa aattttggta tttggcagaa
     3061 aacgcgccag ccgacgcaat tcacctgcca cctgatccgc cagttcacgc gtaggacaca
     3121 gcactaaagc ctgggtttga aatagcgacg catcaatttg ctgtaacaag ccgaggccaa
     3181 aagccgccgt tttgccgctg ccggttttcg cctgcacgcg aacatctttt ccggcaagga
     3241 tcgccggaag cgcggcggcc tgcaccggcg tcatggttaa ataacccaac tcattaaggt
     3301 tcgtgagttg ggcgggaggc aaaacattca gggtagaaaa agcggtcaca atctattctc
     3361 gtggtcatcg acgcaaagtt agcaggcgcg tatcctcgca gatctacgct cacgatgcga
     3421 caatttaatc ggttcttcat cgggtggtgg gtcaggcatg ggttgcgggc gagggatcgg
     3481 atcgggcact ggaacaggat cgctaggaat cggttcaggg acaggaattt gcaaataaat
     3541 aagtgtcgtc atatttccct ccggtcattg ggtggactct taaagggtag acgctgataa
     3601 ataacaggca aaaaaaagcc gactcatcaa agtcggcgtc gtacgaatca attgtgctat
     3661 gcagtaattc aaaaaaggaa gtaagacaat atggagcgca acgcccatcg cttgacgttg
     3721 cattcacctg caatggagat attgccctga atgggtagag agtttattga cttcgctcaa
     3781 actttgcggc gtttttgtat acagacagcc ggaaaaattg cttttgttac aaccatttac
     3841 tacggtgcaa ccataaagca acaccaccaa taagaacaac taacagaata caaaaaattg
     3901 aaaatccgaa ttgccacccg ccgccaggga tcccaccaag gttgacgcca aataaccctg
     3961 tcagaaaggt actgggtaaa aagaccattg ccatcaatga cattgtatag gtacgacgag
     4021 ctaaattttc ctgcatcacc tgagcgattt catccgccat cacaccagtc cgtgctatac
     4081 aggcgtcgat ttcgtcaagg ccgcgcccaa ggcgatcggc aatatcctgc atccgacggc
     4141 gttggtcatc gctcatccac ggcaaacgtt cactggcaag acgagcatag acatcacgtt
     4201 gcggtgccat atagcgacgc atcacgatta attgtttgcg cagcagagcc aggaatccac
     4261 gcggtggaat ttgctgatca aggagattat cttcaaggtc gataatttta tcgtgcagct
     4321 gctcgataaa ttcgctggaa tgatcggtca acgcatcgca cacatccacc agccaccctc
     4381 cgcaatcggt cggacccgtg ccctcttcca gatcgctcac cacatcgtcc agcgccagca
     4441 ctttgcgttg tcgggtcgaa acaattaacc gcccgtccat atatacacgc atggcgacca
     4501 gttgatcggg gcgttcatcg gtgctgccgt ttatacagcg caatgtaata agcgtgcctt
     4561 caccgagacg gctgactcgg ggccgtgtgc tctcgcccgc cagcgcatca cgtacgttat
     4621 tgggaagcag cggtgttgtc gccagccatt gggcgctatc atggtgtaca taattaaggt
     4681 ggagccagca gggatgcgct tcatcaatca catctgtatt ttccagcggt ttaacgccgc
     4741 ctctaccatc cagcatccag gcaaatactg catccgggac attaacgtcc gatcccttaa
     4801 tcgcttccac agtgcctcca tcatcaacgc attattttgt agtctagcct tctggccctg
     4861 ttacgcaaca tctcatcacc ccattaccct gaaatgatta ataaaattct gtctaaattg
//
LOCUS       DAZKHF020000289         4903 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2201S31241
            SAMN43267800-rid25106413.denovo.291, whole genome shotgun sequence.
ACCESSION   DAZKHF020000289 DAZKHF020000000
VERSION     DAZKHF020000289.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267800
            Sequence Read Archive: SRR30307263
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4903)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4903)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 4903)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350581.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350581.2
            Genome Coverage       :: 151x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:21:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,652
            CDSs (total)                      :: 5,557
            Genes (coding)                    :: 5,320
            CDSs (with protein)               :: 5,320
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 237
            CDSs (without protein)            :: 237
            Pseudo Genes (ambiguous residues) :: 0 of 237
            Pseudo Genes (frameshifted)       :: 102 of 237
            Pseudo Genes (incomplete)         :: 130 of 237
            Pseudo Genes (internal stop)      :: 52 of 237
            Pseudo Genes (multiple problems)  :: 41 of 237
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4903
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267800-rid25106413.denovo.291"
                     /serotype="O 26:H11"
                     /isolate="2201S31241"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-01"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            <1..75
                     /locus_tag="ACHOSC_RS21305"
                     /old_locus_tag="pgaptmp_004261"
     CDS             <1..75
                     /locus_tag="ACHOSC_RS21305"
                     /old_locus_tag="pgaptmp_004261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309203.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0060703 - deoxyribonuclease inhibitor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="host-nuclease inhibitor Gam family protein"
                     /protein_id="HGU4550073.1"
                     /translation="RMAEHIRYMVETIAHHQVDIDSEV"
     gene            81..866
                     /gene="bet"
                     /locus_tag="ACHOSC_RS21310"
                     /old_locus_tag="pgaptmp_004262"
     CDS             81..866
                     /gene="bet"
                     /locus_tag="ACHOSC_RS21310"
                     /old_locus_tag="pgaptmp_004262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage recombination protein Bet"
                     /protein_id="HGU4550074.1"
                     /translation="MSTALATLAGKLAERVGMDSVDPQELITTLRQTAFKGDASDAQF
                     IALLIVANQYGLNPWTKEIYAFPDKQNGIVPVVGVDGWSRIINENQQFDGMDFEQDNE
                     SCTCRIYRKDRNHPICVTEWMDECRREPFKTREGREITGPWQSHPKRMLRHKAMIQCA
                     RLAFGFAGIYDKDEAERIVENTAYTAERQPERDITPVNDETMQEINTLLIALDKTWDD
                     DLLPLCSQIFRRDIRASSELTQAEAVKALGFLKQKATEQKVAA"
     gene            863..1543
                     /locus_tag="ACHOSC_RS21315"
                     /old_locus_tag="pgaptmp_004263"
     CDS             863..1543
                     /locus_tag="ACHOSC_RS21315"
                     /old_locus_tag="pgaptmp_004263"
                     /EC_number="3.1.11.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004527 - exonuclease activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lambda exonuclease family protein"
                     /protein_id="HGU4550075.1"
                     /translation="MIPDIILQRTGIDVRAVEQGDDAWHKLRLGVITASEVHNVIAKP
                     RSGKKWPDMKMSYFHTLLAEVCTGVAPEVNAKALAWGKQYENDARTLFEFTSGVNITE
                     SPIIYRDESMRTACSPDGLCSDGNGLELKCPFTSRDFMKFRLGGFEAIKSAYMAQVQY
                     SMWVTRKDAWYFANYDPRMKREGLHYVVIERNEKYMASFDEMVPEFIEKMDEALAEIG
                     FVFGEQWR"
     gene            1540..1722
                     /locus_tag="ACHOSC_RS21320"
                     /old_locus_tag="pgaptmp_004264"
     CDS             1540..1722
                     /locus_tag="ACHOSC_RS21320"
                     /old_locus_tag="pgaptmp_004264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1317 domain-containing protein"
                     /protein_id="HGU4550076.1"
                     /translation="MTHPHDNIRVGAITFVYSVTKRGWVFPGLSVIRNPLKAQRLAEK
                     INNKREAVCTKHLLLS"
     gene            1695..1886
                     /locus_tag="ACHOSC_RS21325"
                     /old_locus_tag="pgaptmp_004265"
     CDS             1695..1886
                     /locus_tag="ACHOSC_RS21325"
                     /old_locus_tag="pgaptmp_004265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1382 family protein"
                     /protein_id="HGU4550077.1"
                     /translation="MHKASPVELRTSIEMAHSLAQIGVRFVPIPVETDEEFHTLATSL
                     SQKLEMMVAKAEADERDQV"
     gene            1963..2178
                     /locus_tag="ACHOSC_RS21330"
                     /old_locus_tag="pgaptmp_004266"
     CDS             1963..2178
                     /locus_tag="ACHOSC_RS21330"
                     /old_locus_tag="pgaptmp_004266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000151206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapA"
                     /protein_id="HGU4550078.1"
                     /translation="MTPQQENALRSIARQANSEIKKARQHFPDKNVDDICRSVLKKHR
                     ETVTLMGFTPTHLSLAIGMLNGVFKER"
     gene            2277..2495
                     /locus_tag="ACHOSC_RS21335"
                     /old_locus_tag="pgaptmp_004267"
     CDS             2277..2495
                     /locus_tag="ACHOSC_RS21335"
                     /old_locus_tag="pgaptmp_004267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016246897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TraR/DksA family transcriptional regulator"
                     /protein_id="HGU4550079.1"
                     /translation="MADIIDSASEIEELQRNTAIKMRRQNYQTVSATHCCECGDPIDE
                     RRRLAVQGCRTCASCQEEIELKNKQWGL"
     gene            2543..2821
                     /locus_tag="ACHOSC_RS21340"
                     /old_locus_tag="pgaptmp_004268"
     CDS             2543..2821
                     /locus_tag="ACHOSC_RS21340"
                     /old_locus_tag="pgaptmp_004268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016248605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4222 domain-containing protein"
                     /protein_id="HGU4550080.1"
                     /translation="MFRIIFPNTWYVDHHGTPCKILRSTHNKVHYIRKGRTCIASMFR
                     FNHDFEPVNKADADRIAEEIETAEHIKKLRDMRSKSRGNHGIIQPHTR"
     gene            2793..2858
                     /locus_tag="ACHOSC_RS21345"
                     /old_locus_tag="pgaptmp_004269"
                     /pseudo
     CDS             2793..2858
                     /locus_tag="ACHOSC_RS21345"
                     /old_locus_tag="pgaptmp_004269"
                     /inference="COORDINATES: protein motif:HMM:NF024139.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
     gene            3020..4183
                     /gene="intD"
                     /locus_tag="ACHOSC_RS21350"
                     /old_locus_tag="pgaptmp_004270"
     CDS             3020..4183
                     /gene="intD"
                     /locus_tag="ACHOSC_RS21350"
                     /old_locus_tag="pgaptmp_004270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="integrase"
                     /protein_id="HGU4550081.1"
                     /translation="MSLFRRNEIWYASYSLPGGKRIKESLGTKDKRQAQELHDKRKAE
                     LWRVEKLGDLPDVTFEEACLRWLEEKADKKSLDSDKSRIEFWLEHFEGIRLKDISEAK
                     IYSAVSRMHNRKTKEIWKQKVQAAIRKGKEPPVYEPKPVSTQTKAKHLAMIKAILRAA
                     ERDWKWLEKAPVIKIPAVRNKRVRWLEKEEAKRLIDECPEPLKSVVKFALATGLRKSN
                     IINLEWQQIDMQRRVAWVNPEESKSNRAIGVALNDTACKVLRDQIGKHHKWVFVHTKA
                     AKRADGTSTPAVRKMRIDSKTSWLSACRRAGIEDFRFHDLRHTWASWLIQSGVPLSVL
                     QEMGGWESIEMVRRYAHLAPNHLTEHARKIDDIFGDNVPNMSHSGIMEDIKKA"
     gene            complement(4199..4275)
                     /locus_tag="ACHOSC_RS21355"
                     /old_locus_tag="pgaptmp_004271"
     tRNA            complement(4199..4275)
                     /locus_tag="ACHOSC_RS21355"
                     /old_locus_tag="pgaptmp_004271"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(4239..4241),aa:Arg,seq:tct)
     gene            4518..>4903
                     /gene="fimZ"
                     /locus_tag="ACHOSC_RS21360"
                     /old_locus_tag="pgaptmp_004272"
     CDS             4518..>4903
                     /gene="fimZ"
                     /locus_tag="ACHOSC_RS21360"
                     /old_locus_tag="pgaptmp_004272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbria biosynthesis transcriptional regulator
                     FimZ"
                     /protein_id="HGU4550082.1"
                     /translation="MKPTSVIIMDTHPIIRMSIEVLLQKNSELQIVLKTDDYRITIDY
                     LRTRPVDLIIMDIDLPGTDGFTFLKRIKQIQSTVKVLFLSSKSECFYAGRAIQAGANG
                     FVSKCNDQNDIFHAVQMILSGYTFFP"
BASE COUNT         1437 a         1132 c         1221 g         1113 t
ORIGIN      
        1 cgcatggcag aacacatccg gtacatggtt gaaaccattg ctcaccacca ggttgatatt
       61 gattcagagg tataaaacgg atgagtacag cactcgcaac gctggcaggg aagctggctg
      121 aacgtgtcgg catggattct gtcgacccac aggaactgat caccactctt cgccagacgg
      181 catttaaagg tgatgccagc gatgcgcagt tcatcgcatt gttgatcgtc gccaaccagt
      241 acggccttaa tccgtggacg aaagaaattt acgccttccc tgataagcag aacggcattg
      301 ttccggtggt gggcgttgat ggctggtccc gcatcatcaa tgaaaaccag cagtttgatg
      361 gcatggactt tgagcaggac aatgaatcct gtacatgccg gatttaccgc aaggaccgta
      421 atcatccgat ctgcgttacc gaatggatgg atgaatgccg ccgcgaacca ttcaaaaccc
      481 gcgaaggcag agaaatcacg gggccgtggc agtcgcatcc caaacggatg ttacggcata
      541 aagccatgat tcagtgtgcc cgtctggcct tcggatttgc tggtatctat gacaaggatg
      601 aagccgagcg cattgtcgaa aatactgcat acactgcaga acgtcagcca gaacgcgaca
      661 tcactccggt taacgatgaa accatgcagg agattaacac tctgctgatc gccctggata
      721 aaacatggga tgacgactta ttgccgctct gttcccagat atttcgccgc gacattcgcg
      781 catcgtcaga actgacacag gccgaagcag tgaaagctct tggattcctg aaacagaaag
      841 ccactgagca gaaggtggca gcatgatacc ggacattatc ctgcagcgta ccgggatcga
      901 cgtgagagct gtcgaacagg gggatgatgc atggcacaaa ttacggctcg gcgtcatcac
      961 cgcttcagaa gttcacaacg tgatagcaaa gccccgctca ggaaagaagt ggcctgacat
     1021 gaaaatgtcc tacttccaca ccctgctggc tgaggtttgc accggtgtgg ctccggaagt
     1081 taatgctaag gcgctggcct ggggaaaaca gtacgagaac gacgccagaa ccctgtttga
     1141 attcacttcc ggcgtgaata ttactgaatc cccgatcatc tatcgcgacg aaagtatgcg
     1201 caccgcctgc tctcccgatg gtttatgcag tgacggcaac ggccttgaac tgaaatgccc
     1261 gtttacctcc cgggatttca tgaaattccg gctcggtggt ttcgaggcaa taaaatcggc
     1321 ttacatggcc caggtgcagt acagcatgtg ggtgacgcga aaagatgcct ggtactttgc
     1381 caactatgac ccgcgcatga agcgtgaagg cctgcattat gtcgtgattg agcggaatga
     1441 aaagtacatg gcgagttttg acgagatggt gccggagttc atcgaaaaaa tggacgaggc
     1501 actggctgaa attggttttg tatttgggga gcaatggcga tgacgcatcc tcacgataat
     1561 atccgggtag gcgcgatcac tttcgtctac tccgttacaa agcgaggctg ggtatttccc
     1621 ggcctttctg ttatcagaaa tccactgaaa gcacagcggc tggctgagaa gataaataat
     1681 aaacgggagg cggtatgcac aaagcatctc ctgttgagtt aagaacgagt attgagatgg
     1741 cacatagcct tgctcaaatt ggagtcaggt ttgtgccaat accagtagaa acagacgaag
     1801 aatttcatac gttagccaca tccctttcac aaaagctgga aatgatggtg gcgaaagcag
     1861 aagcagatga gagagaccag gtatgacaac cactgaatgc atttttctgg cagcgggctt
     1921 catattctgt gtgcttatgc ttgccgacat ggggcttgtt caatgacacc tcagcaagaa
     1981 aacgcccttc gcagcattgc ccgtcaggct aattctgaaa tcaaaaaagc cagacagcat
     2041 tttccggata aaaacgtcga tgacatttgc cgtagcgtac taaagaagca ccgcgaaacg
     2101 gtaacgctga tgggattcac accgactcat ttaagcctgg cgatcggcat gttaaacggc
     2161 gtctttaagg aacggtgaac atgaaaagca aaatcatcag ggagctacag gctccttttt
     2221 tattgttcgc attcatcctc aagcgtatta accaacaatt cagggattaa tggaagatgg
     2281 cagacatcat tgattcagca tcagaaatcg aagaattaca gcgcaataca gcaataaaaa
     2341 tgcgtcgtca gaactaccag actgtatccg caactcattg ttgtgagtgt ggcgatccga
     2401 tagatgagcg aagacgcctg gctgttcagg gttgtcggac ttgtgcaagt tgccaggagg
     2461 agatcgaact taagaacaaa caatggggac tgtgatggcc tcaaagcagc aaatttcaac
     2521 atcgtccaac tgaggtgtaa aaatgttcag aatcattttt cctaacacct ggtacgtcga
     2581 ccaccacggc actccctgca aaatcctgcg ttctacccac aacaaagttc actacatccg
     2641 aaaaggcaga acatgtatcg ccagcatgtt ccgctttaat catgactttg aacctgtgaa
     2701 taaagctgat gcagatcgga tagcagaaga gatcgaaacg gcagaacaca ttaagaagtt
     2761 acgtgacatg cgttcaaaaa gcagaggtaa ccatggaatc atacagcctc acactcgatg
     2821 aggcctgtca gtttcttaag atatccagac caaccgccac caactggata cgaacaggcc
     2881 gcctacaggc aacacgtaaa gatccaacca agccaaaatc tccttacctc acaacacggc
     2941 aagcctgcat tgcggcgctt cagtctccgc tgcatactgt ccaggtgagc gcgggtgatg
     3001 gcataacaga ggaaagaaaa tgtcactctt ccgcagaaat gaaatatggt atgcctcgta
     3061 ttcgctcccg ggcgggaaac gaattaagga atctcttggc acaaaggaca agcggcaagc
     3121 tcaggagttg cacgacaagc gaaaagcaga actctggcga gtagaaaagc taggggattt
     3181 acctgatgtc acttttgaag aggcctgcct aagatggctt gaggaaaaag ctgataaaaa
     3241 atctctcgat tcagataaaa gccggattga gttctggctt gaacattttg agggtataag
     3301 gcttaaagat atctcggagg caaagattta ctctgctgta agcagaatgc ataacagaaa
     3361 gacgaaagaa atatggaaac agaaagttca ggccgccatc aggaaaggta aagaaccgcc
     3421 tgtttatgaa ccaaagccag tatcaactca gacaaaggca aagcatcttg ccatgataaa
     3481 ggccattctc cgtgctgcag aacgcgactg gaagtggctg gaaaaagcgc ctgtcatcaa
     3541 gataccagcg gtcagaaaca agcgagtcag atggctggaa aaggaggaag caaaacgcct
     3601 tattgatgag tgccccgaac cactgaaatc tgtcgtcaag tttgcgctgg caactggtct
     3661 gagaaagtcg aacatcataa atctggaatg gcaacaaatc gacatgcagc gacgagttgc
     3721 ctgggtgaat ccagaagaga gcaaatcaaa ccgcgccatt ggtgtggcgc tgaacgatac
     3781 cgcctgtaaa gtgttgcgtg atcaaatagg caagcatcac aaatgggtgt ttgtacatac
     3841 caaggcggct aagcgagcag atggaacatc aacgcctgcg gtcaggaaga tgcgcatcga
     3901 cagcaagaca tcatggctat cagcttgtcg tcgtgcagga attgaagatt tccgtttcca
     3961 tgacctcaga cacacctggg caagctggct gattcagtca ggcgtcccat tatcagtgct
     4021 tcaggaaatg ggcggatggg agtccataga aatggttcgt aggtatgctc accttgcgcc
     4081 taatcatttg acagagcatg cgaggaaaat agacgacatt tttggtgata atgtcccaaa
     4141 tatgtcccac tctggaatta tggaggatat aaagaaggcg taactgattg aattgtaatg
     4201 gcgcgccctg caggattcga acctgcggcc cacgacttag aaggtcgttg ctctatccaa
     4261 ctgagctaag ggcgcgttga taccgcaatg cggtgtaatc gcgtgaatta tacggtcaac
     4321 ccttgctgag tcaatggctt ttgatctggt tgctgaacaa gtgaacgacc gcgtctgatt
     4381 ttctgattta tttcgctata gcggcaaaca aacacacacc gctgcgcgtc tgaatcaaga
     4441 aaacccgtat tttcatgtat caaagtacaa tttaccgccc taacggaaaa ttgtccgctc
     4501 ctatgagact ggtaactatg aaaccaacgt cggtgatcat tatggatact catcctatca
     4561 tcagaatgtc tattgaagtt ctgttgcaaa aaaacagtga attgcagatt gtcctgaaaa
     4621 cggatgatta ccgcataacc atcgattatc tccgaacccg tcctgttgat ttaatcatta
     4681 tggatataga cttgcccgga acagacggtt ttaccttcct gaaaaggatc aaacaaatcc
     4741 agagcacagt gaaagtgtta tttttatcat cgaaatcaga atgcttttat gctggcagag
     4801 cgatacaagc tggtgctaac ggttttgtca gtaaatgcaa tgatcagaat gatatttttc
     4861 atgccgttca gatgatcctc tccggataca cgttttttcc cag
//
LOCUS       DAZKHF020000290         4874 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2201S31241
            SAMN43267800-rid25106413.denovo.292, whole genome shotgun sequence.
ACCESSION   DAZKHF020000290 DAZKHF020000000
VERSION     DAZKHF020000290.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267800
            Sequence Read Archive: SRR30307263
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4874)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4874)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 4874)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350581.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350581.2
            Genome Coverage       :: 151x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:21:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,652
            CDSs (total)                      :: 5,557
            Genes (coding)                    :: 5,320
            CDSs (with protein)               :: 5,320
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 237
            CDSs (without protein)            :: 237
            Pseudo Genes (ambiguous residues) :: 0 of 237
            Pseudo Genes (frameshifted)       :: 102 of 237
            Pseudo Genes (incomplete)         :: 130 of 237
            Pseudo Genes (internal stop)      :: 52 of 237
            Pseudo Genes (multiple problems)  :: 41 of 237
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4874
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267800-rid25106413.denovo.292"
                     /serotype="O 26:H11"
                     /isolate="2201S31241"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-01"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            complement(<1..458)
                     /locus_tag="ACHOSC_RS21365"
                     /old_locus_tag="pgaptmp_004273"
     CDS             complement(<1..458)
                     /locus_tag="ACHOSC_RS21365"
                     /old_locus_tag="pgaptmp_004273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414921.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YaiI/YqxD family protein"
                     /protein_id="HGU4550083.1"
                     /translation="MTIWVDADACPNVIKEILYRAAERMQMPLVLVANQSLRVPPSRF
                     IRTLRVAAGFDVADNEIVRQCEAGDLVITADIPLAAEAIEKGAAALNPRGERYTPATI
                     RERLTMRDFMDTLRASGIQTGGPDSLSQRDRQAFAAELEKWWLEVQRSRG"
     gene            578..1387
                     /gene="proC"
                     /locus_tag="ACHOSC_RS21370"
                     /old_locus_tag="pgaptmp_004274"
     CDS             578..1387
                     /gene="proC"
                     /locus_tag="ACHOSC_RS21370"
                     /old_locus_tag="pgaptmp_004274"
                     /EC_number="1.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004735 - pyrroline-5-carboxylate
                     reductase activity [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyrroline-5-carboxylate reductase"
                     /protein_id="HGU4550084.1"
                     /translation="MEKKIGFIGCGNMGKAILGGLIASGQVLPGQIWVYTPSPDKVAA
                     LHDQFGINAAESAQEVAQIADIIFAAVKPGIMIKVLSEITSSLNKDSLVVSIAAGVTL
                     DQLARALGHDRKIIRAMPNTPALVNAGMTSVTPNALVTPEDTADVLNIFRCFGEAEVI
                     AEPMIHPVVGVSGSSPAYVFMFIEAMADAAVLGGMPRAQAYKFAAQAVMGSAKMVLET
                     GEHPGALKDMVCSPGGTTIEAVRVLEEKGFRAAVIEAMTKCMEKSEKLSKS"
     gene            complement(1404..2519)
                     /gene="adrA"
                     /locus_tag="ACHOSC_RS21375"
                     /old_locus_tag="pgaptmp_004275"
                     /gene_synonym="dgcC"
     CDS             complement(1404..2519)
                     /gene="adrA"
                     /locus_tag="ACHOSC_RS21375"
                     /old_locus_tag="pgaptmp_004275"
                     /gene_synonym="dgcC"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase AdrA"
                     /protein_id="HGU4550085.1"
                     /translation="MFPKIMNDENFFKKAAAHGEEPPLTPQNEHQRSGLRFARRVRLP
                     RAVGLAGMFLPIASTLVSHPPPGWWWLVLVGWAFVWPHLAWQIASRAVDPLSREIYNL
                     KTDAVLAGMWVGVMGVNVLPSTAMLMIMCLNLMGAGGPRLFVAGLVLMVVSCLVTLEL
                     TGITVSFNSAPLEWWLSLPIIVVYPLLFGWVSYQTATKLAEHKRRLQVMSTRDGMTGV
                     YNRRHWETMLRNEFDNCRRHNRDATLLIIDIDHFKSINDTWGHDVGDEAIVALTRQLQ
                     ITLRGSDVIGRFGGDEFAVIMSGTPAESAITAMLRVREGLNTLRLPNTPQVTLRISVG
                     VAPLNPQMSHYREWLKSADLALYKAKKAGRNRTEVAA"
     gene            complement(2621..2941)
                     /gene="psiF"
                     /locus_tag="ACHOSC_RS21380"
                     /old_locus_tag="pgaptmp_004276"
     CDS             complement(2621..2941)
                     /gene="psiF"
                     /locus_tag="ACHOSC_RS21380"
                     /old_locus_tag="pgaptmp_004276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414918.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate starvation-inducible protein PsiF"
                     /protein_id="HGU4550086.1"
                     /translation="MKITLLVTLLFGLVFLTTVGAAERTLTPQQQRMTSCNQQATAQA
                     LKGDARKTYMSDCLKNSKSAPGEKSLTPQQQKMRECNNQATQQSLKGDDRNKFMSACL
                     KKAA"
     gene            complement(3060..4475)
                     /gene="phoA"
                     /locus_tag="ACHOSC_RS21385"
                     /old_locus_tag="pgaptmp_004277"
     CDS             complement(3060..4475)
                     /gene="phoA"
                     /locus_tag="ACHOSC_RS21385"
                     /old_locus_tag="pgaptmp_004277"
                     /EC_number="3.1.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414917.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase"
                     /protein_id="HGU4550087.1"
                     /translation="MKQSTIALALLSLLFTPVTKARTPEMPVLENRAAQGDITASGGA
                     RRLTGDQTAALRDSLSDKPAKNIILLIGDGMGDSEITAARNYAEGAGGFFKGIDALPL
                     TGQYTHYALNKKTGKPDYVTDSAASATAWSTGVKTYNGALGVDIHEKDHPTILEMAKA
                     AGLATGNVSTAELQDATPAALVAHVTSRKCYGPSATSEKCPGNALEKGGKGSITEQLL
                     NARADVTLGGGAKTFAETATAGEWQGKTLREQAQARGYQLVSDAASLNSVTEANQQKP
                     LLGLFADGNMPVRWLGPKATYHGNIDKPAVTCTPNPQRNDSVPTLAQMTDKAIELLSK
                     NEKGFFLQVEGASIDKQDHAANPCGQIGETVDLDEAVQRALEFAKKDGNTLVIVTADH
                     AHASQIVAPDTKAPGLTQALNTKDGAVMVMSYGNSEEDSQEHTGSQLRIAAYGPHAAN
                     VVGLTDQTDLFYTMKAALGLK"
     gene            complement(4576..4836)
                     /gene="iraP"
                     /locus_tag="ACHOSC_RS21390"
                     /old_locus_tag="pgaptmp_004278"
     CDS             complement(4576..4836)
                     /gene="iraP"
                     /locus_tag="ACHOSC_RS21390"
                     /old_locus_tag="pgaptmp_004278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anti-adapter protein IraP"
                     /protein_id="HGU4550088.1"
                     /translation="MKNLIAELLFKLAQKEEESKELCAQVEALEIIVTAMLRNMAQND
                     QQRLIDQVEGALYEVKPDASIPDDDTELLRDYVKKLLKHPRQ"
BASE COUNT         1130 a         1333 c         1210 g         1201 t
ORIGIN      
        1 tagccacgac tacgttgcac ttccagccac cacttctcca gctccgcggc aaaggcctgg
       61 cggtcacgtt gtgaaaggct atctggtccg ccggtctgga tcccactggc acgtaaggta
      121 tccataaaat cgcgcatcgt caggcgctca cgaatggtcg ctggcgtgta acgttcgccg
      181 cgcggattaa gcgccgctgc gcctttctcg atggcttcag cagccaaagg tatatctgcg
      241 gtgatcacca aatcgcccgc ttcacactgc cggacaattt cgttatcggc aacgtcgaaa
      301 cctgccgcga cgcgcagcgt acgaataaat cgcgatggcg gcacgcgtaa actctggttt
      361 gctaccagta ccagcggcat ctgcatacgt tccgccgcgc gatacaaaat ctctttaatt
      421 acattgggac acgcgtcggc atccacccaa attgtcataa agtcatcctt tgttgggtaa
      481 tcctctattg tgtcgcgctt ttgccttccg gcatagttct gtttatgctt ctgccagcga
      541 ttatcaaaac aatgaatttc acggcaggag tgaggcaatg gaaaagaaaa tcggttttat
      601 tggctgcggc aatatgggaa aagccattct cggcggtctg attgccagcg gtcaggtgct
      661 tccagggcaa atctgggtat acaccccctc cccggataaa gtcgccgccc tgcatgacca
      721 gttcggcatc aacgccgcag aatcggcgca agaagtggcg caaatcgccg acatcatttt
      781 tgctgccgtt aaacctggca tcatgattaa agtgcttagc gaaatcacct ccagcctgaa
      841 taaagactct ctggtcgttt ctattgctgc aggtgtcacg ctcgaccagc ttgcccgcgc
      901 gctgggccat gaccggaaaa ttatccgcgc catgccgaac actcccgcgc tggttaatgc
      961 cgggatgacc tccgtaacgc caaacgcgct ggtaacccca gaagataccg ctgatgtgct
     1021 gaatattttc cgctgctttg gcgaagcgga agtaattgct gagccgatga tccacccggt
     1081 ggtcggtgtg agcggttctt cgccagccta cgtatttatg tttatcgaag cgatggccga
     1141 cgccgccgtg ctgggcggga tgccacgcgc ccaggcgtat aaatttgccg ctcaggcggt
     1201 aatgggttcc gcaaaaatgg tgctggaaac gggagaacat ccgggggcac tgaaagatat
     1261 ggtctgctca ccgggaggca ccaccattga agcggtacgc gtactggaag agaaaggctt
     1321 ccgtgccgca gtgatcgaag cgatgacgaa gtgtatggaa aaatcagaaa aactcagcaa
     1381 atcctgatga ctttcgccgg acgtcaggcc gccacttcgg tgcggttacg tccggctttc
     1441 tttgctttgt aaagcgccaa atctgccgat ttcaaccact cacgatagtg actcatttgt
     1501 gggttcagcg gcgcaacccc cacactaatc cgtaaagtta cctgtggcgt attcggcaag
     1561 cgtaacgtgt ttagcccttc acgcacccgt aacatggcgg taatggcgct ctcagctggc
     1621 gtaccggaca tgattactgc aaactcatcg ccgccaaacc gaccaatcac atcgctaccg
     1681 cgcagggtaa tttgtaactg tcgggtaagc gccacaatcg cttcatcgcc cacatcatga
     1741 ccccaggtat cgttgatgct cttgaaatgg tcgatatcga taatcagtaa cgttgcatcg
     1801 cgattatgcc gccgacagtt atcaaattca ttgcgtaaca tagtttccca atgacgtcgg
     1861 ttatacacgc ccgtcatgcc gtcgcgggta ctcatgacct gcaacctgcg tttatgttcc
     1921 gccagtttgg ttgccgtctg gtagctgacc cagccaaaca gcagaggata aacgacaata
     1981 atgggaaggg agagccacca ttccagcggc gcactattga acgacacggt aatgcccgta
     2041 agctcgaggg tgacaaggca ggaaaccacc atcaacacca gacccgcgac aaacagacgg
     2101 gggccgcctg cccccatcaa attcagacac ataatcatca acatagcggt ggaaggcagc
     2161 acgtttacgc ccattacgcc tacccacatt cccgctaata ctgcatcggt ttttaagttg
     2221 taaatttccc ggctaagcgg atcgacggcc ctgctcgcta tctgccaggc taaatgcggc
     2281 cagacgaacg cccagccgac caacaccagc caccaccagc ccggcggcgg gtgcgaaacc
     2341 agcgttgaag caatcggtaa gaacatgcca gccaggccaa ccgcacgggg tagtctgacg
     2401 cgacgggcga agcgcagccc ggaccgctga tgttcgtttt gaggagttaa aggaggttcc
     2461 tccccatgcg ccgccgcttt tttgaaaaag ttttcatcat tcattatttt tgggaacatt
     2521 cagaaacaat tttcccaaat tatagagacg gggctaaagg cagaagtatg aaatttcggg
     2581 tgagccactt tcgtagctca cccgttagca ctatcaggta tcaggcggct ttcttgaggc
     2641 aggcactcat aaacttatta cgatcatcac ctttcagaga ttgttgcgtt gcttgattat
     2701 tacattcgcg catcttttgc tgctgtggcg tcaaactttt ttcaccaggc gcagacttgc
     2761 tgttcttcag gcaatcactc atgtaggtct tacgagcatc ccctttcaac gcctgcgccg
     2821 tcgcctgctg attacaggat gtcatacgct gttgttgtgg ggttaaggtt ctctcggcag
     2881 cgccgacggt ggttaaaaaa accagaccaa aaagcaaggt aaccagtaat gttattttca
     2941 tagcaccatc cctcttcatg ttttaaccat gagcgtatgc gcccgtgatc tgccattaag
     3001 tctggttgct aacagcaaaa aaaccacccg gcagcgaaaa ttcactgccg ggcgcggttt
     3061 tatttcagcc ccagggcggc tttcatggtg tagaagagat cggtctggtc ggtcagtcca
     3121 acaacattgg cggcatgcgg gccatacgcc gcaatacgca actgactgcc ggtatgttct
     3181 tgtgaatcct cttcggagtt cccgtagctc atcaccatca ctgcgccatc tttggtattt
     3241 agcgcctggg tgaggcccgg agctttggta tccggcgcga caatctggct ggcgtgggcg
     3301 tgatcagcgg tgactatgac cagcgtgttg ccatcctttt tagcgaattc cagcgcccgt
     3361 tgtacggctt catcgagatc gaccgtctcg ccaatttgcc cacaaggatt cgcagcgtga
     3421 tcctgtttat cgattgacgc accttcaact tgcaggaaaa atcctttctc atttttactc
     3481 aacaattcaa tggctttgtc ggtcatctgc gccagggttg gtacactgtc attacgttgc
     3541 ggattaggcg tacaggtgac tgcgggctta tcgatattgc cgtggtacgt tgctttcggt
     3601 cctagccagc gcactggcat attgccgtca gcaaacagtc caagcagagg tttttgctga
     3661 ttcgcttccg tcaccgaatt cagtgaggca gcatcgctga ccaactgata accacgcgcc
     3721 tgtgcctgtt cacgcagcgt ttttccctgc cattcaccgg cggttgccgt ttcagcaaag
     3781 gtttttgcgc caccgccaag cgtaacgtcg gcacgagcgt taagcagctg ttcggtaatc
     3841 gatccttttc cgcctttttc cagagcgtta cccggacatt tttcactggt cgcgctcgga
     3901 ccgtagcatt tgcgcgaggt cacatgcgcc accagcgcag cgggcgtggc atcctgcaac
     3961 tctgcggtag aaacgttacc ggtcgccaga cctgcggctt ttgccatttc cagaatcgtt
     4021 gggtgatctt tttcgtgaat atcgacgccc agcgcgccgt tataggtttt gacaccggtt
     4081 gaccaggcgg ttgctgatgc agccgaatcg gtgacgtagt ccggtttgcc ggttttttta
     4141 ttcagcgcat agtgagtgta ttgcccggta agcggtaagg catcgatacc tttaaaaaag
     4201 ccgcccgcac cttcggcata attacgtgcg gctgtaattt ccgaatcccc catcccatcg
     4261 ccaatcagca aaataatatt ttttgcaggt ttatcgctaa gagaatcacg cagagcggca
     4321 gtctgatcac ccgttaaacg gcgagcaccg ccggatgcag taatatcgcc ctgagcagcc
     4381 cggttttcca gaacaggcat ttctggtgtc cgggcttttg tcacaggggt aaacagtaac
     4441 gataagagtg ccagtgcaat agtgctttgt ttcactttat tttctccatg tacaaataca
     4501 ttaaaaaata aaaacaaagc gactataagt ctcggccgtg acaactttat gacagctgtt
     4561 gaaaagatta actttttact gacgaggatg cttcaataac ttctttacgt aatcgcgcag
     4621 cagctccgta tcgtcgtcag gaatgctggc atcgggcttt acctcgtaca gcgccccctc
     4681 tacctgatca atcaagcgct gttggtcatt ttgcgccata ttgcgaagca ttgcagtgac
     4741 gataatctcc aaagcttcta cctgcgcaca cagttctttc gactcttctt ctttttgggc
     4801 aagcttaaat aacaactcag caatgagatt tttcatgtct gtatttcctt atccaaagta
     4861 tggagaagtt aaca
//
LOCUS       DAZKHF020000291         4860 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2201S31241
            SAMN43267800-rid25106413.denovo.293, whole genome shotgun sequence.
ACCESSION   DAZKHF020000291 DAZKHF020000000
VERSION     DAZKHF020000291.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267800
            Sequence Read Archive: SRR30307263
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4860)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4860)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 4860)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350581.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350581.2
            Genome Coverage       :: 151x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:21:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,652
            CDSs (total)                      :: 5,557
            Genes (coding)                    :: 5,320
            CDSs (with protein)               :: 5,320
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 237
            CDSs (without protein)            :: 237
            Pseudo Genes (ambiguous residues) :: 0 of 237
            Pseudo Genes (frameshifted)       :: 102 of 237
            Pseudo Genes (incomplete)         :: 130 of 237
            Pseudo Genes (internal stop)      :: 52 of 237
            Pseudo Genes (multiple problems)  :: 41 of 237
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4860
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267800-rid25106413.denovo.293"
                     /serotype="O 26:H11"
                     /isolate="2201S31241"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-01"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            163..1137
                     /gene="hemB"
                     /locus_tag="ACHOSC_RS21395"
                     /old_locus_tag="pgaptmp_004279"
     CDS             163..1137
                     /gene="hemB"
                     /locus_tag="ACHOSC_RS21395"
                     /old_locus_tag="pgaptmp_004279"
                     /EC_number="4.2.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308450.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004655 - porphobilinogen synthase
                     activity [Evidence IEA];
                     GO_process: GO:0033014 - tetrapyrrole biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="porphobilinogen synthase"
                     /protein_id="HGU4550089.1"
                     /translation="MTDLIQRPRRLRKSPALRAMFEETTLSLNDLVLPIFVEEEIDDY
                     KAVEAMPGVMRIPEKHLAREIERIANAGIRSVMTFGISHHTDETGSDAWREDGLVARM
                     SRICKQTVPEMIVMSDTCFCEYTSHGHCGVLCEHGVDNDATLENLGKQAVVAAAAGAD
                     FIAPSAAMDGQVQAIRQALDAAGFKDTAIMSYSTKFASSFYGPFREAAGSALKGDRKS
                     YQMNPMNRREAIRESLLDEAQGADCLMVKPAGAYLDIVRELRERTELPIGAYQVSGEY
                     AMIKFAALAGAIDEEKVVLESLGSIKRAGADLIFSYFALDLAEKKILR"
     gene            complement(1243..2094)
                     /gene="tauD"
                     /locus_tag="ACHOSC_RS21400"
                     /old_locus_tag="pgaptmp_004280"
     CDS             complement(1243..2094)
                     /gene="tauD"
                     /locus_tag="ACHOSC_RS21400"
                     /old_locus_tag="pgaptmp_004280"
                     /EC_number="1.14.11.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="taurine dioxygenase"
                     /protein_id="HGU4550090.1"
                     /translation="MSERLSITPLGPYIGAQISGADLTRPLSDNQFEQLYHAVLRHQV
                     VFLRDQTIAPQQQRALAQRFGELHIHPVYPHAEGVDEIIVLDTHNDNPPDNDNWHTDV
                     TFIETPPAGAILAAKELPSTGGDTLWTSGIAAYEALSVPFRQLLSGLRAEHDFRKSFP
                     EYKYRKTEEEHQRWREAVAKNPPLLHPVVRTHPVSGKQALFVNEGFTTRIVDVSEKES
                     EALLGFLFAHITKPEFQVRWRWQPNDIAIWDNRVTQHYANADYLPQRRIMHRATILGD
                     KPFYRAG"
     gene            complement(2091..2918)
                     /gene="tauC"
                     /locus_tag="ACHOSC_RS21405"
                     /old_locus_tag="pgaptmp_004281"
     CDS             complement(2091..2918)
                     /gene="tauC"
                     /locus_tag="ACHOSC_RS21405"
                     /old_locus_tag="pgaptmp_004281"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015411 - ABC-type taurine transporter
                     transporter activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="taurine ABC transporter permease TauC"
                     /protein_id="HGU4550091.1"
                     /translation="MSVLINEKLHSRRLKWRWPLSRQVTLSIGTLAVLLTVWWTVAAL
                     QLISPLFLPPPQQVLAKLLTIAGPQGFMDATLWQHLAASLTRIVLALLAAVVIGIPVG
                     IAMGLSPTVRGILDPVIELYRPVPPLAYLPLMVIWFGIGETSKILLIYLAIFAPVAMS
                     ALAGVKSAQQVRIRAAQSLGASRAQVLWFVILPGALPEILTGLRIGLGVGWSTLVAAE
                     LIAATRGLGFMVQSAGEFLATDVVLAGIAVIAIIAFLLELGLRALQRRLTPWHGEVQ"
     gene            complement(2915..3682)
                     /gene="tauB"
                     /locus_tag="ACHOSC_RS21410"
                     /old_locus_tag="pgaptmp_004282"
     CDS             complement(2915..3682)
                     /gene="tauB"
                     /locus_tag="ACHOSC_RS21410"
                     /old_locus_tag="pgaptmp_004282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015411 - ABC-type taurine transporter
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="taurine ABC transporter ATP-binding subunit"
                     /protein_id="HGU4550092.1"
                     /translation="MLQISHLYADYGGKPALEDINLTLESGELLVVLGPSGCGKTTLL
                     NLIAGFVPYQHGSIQLAGKRIEGPGAERGVVFQNEGLLPWRNVQDNVAFGLQLAGIEK
                     MQRLEIAHQMLKKVGLEGAEKRYIWQLSGGQRQRVGIARALAANPQLLLLDEPFGALD
                     AFTRDQMQTLLLKLWQETGKQVLLITHDIEEAVFMATELVLLSPGPGRVLERLPLNFA
                     RRFVAGESSRSIKSDPQFIAMREYVLSRVFEQREAFS"
     gene            complement(3695..4657)
                     /gene="tauA"
                     /locus_tag="ACHOSC_RS21415"
                     /old_locus_tag="pgaptmp_004283"
     CDS             complement(3695..4657)
                     /gene="tauA"
                     /locus_tag="ACHOSC_RS21415"
                     /old_locus_tag="pgaptmp_004283"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414899.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0015411 - ABC-type taurine transporter
                     transporter activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="taurine ABC transporter substrate-binding
                     protein"
                     /protein_id="HGU4550093.1"
                     /translation="MAISSRNTLLAALAFIAFQAQAVNVTVAYQTSAEPAKVAQADNT
                     FAKESGATVDWRKFDSGASIVRALASGDVQIGNLGSSPLAVAASQQVPIEVFLLASKL
                     GNSEALVVKKTISKPEDLIGKRIAVPFISTTHYSLLAALKHWGIKPGQVEIVNLQPPA
                     IIAAWQRGDIDGAYVWAPAVNALEKDGKVLTDSEQVGQWGAPTLDVWVVRKDFAEKHP
                     EVVKAFAKSAIDAQQPYIANPDAWLKQPENISKLARLSGVPEGDVPGLVKGNTYLTPQ
                     QQTAELTGPVNKAIIDTAQFLKEQGKVPAVANDYSQYVTSRFVQ"
BASE COUNT         1120 a         1405 c         1243 g         1092 t
ORIGIN      
        1 aaatattttc agtgaatatg aaattattgc tatttctatt acaaagatgt cctggtctct
       61 ctttcattct cttcttgtca acatcacgac aactttcgta aaacactcct gccccgcttc
      121 aggtatactg tgcaccttaa ttccacaaac atcaggcaga ccatgacaga cttaatccaa
      181 cgccctcgtc gcctgcgcaa atctcctgcg ctgcgcgcta tgtttgaaga gacaacactt
      241 agccttaacg acctggtgtt gccgatcttt gttgaagaag aaattgacga ctacaaagcc
      301 gttgaagcca tgccaggcgt gatgcgcatt ccagagaaac atctggcacg cgaaattgaa
      361 cgcatcgcca acgccggtat ccgttccgta atgacttttg gcatctctca ccataccgat
      421 gaaaccggca gcgacgcctg gcgggaagat ggactggtgg cgcgtatgtc gcgcatctgc
      481 aagcagactg tgccagaaat gatcgtcatg tcagacactt gtttctgtga atacacttct
      541 cacggtcact gcggtgtgct gtgcgagcat ggcgtcgaca acgacgcaac tctggaaaat
      601 ttaggcaagc aagccgtggt tgcagctgct gcaggcgcag acttcatcgc cccttccgcc
      661 gcgatggatg gccaggtaca ggctattcgc caggcgctgg acgctgcagg ctttaaagat
      721 acggcgatta tgtcgtattc gaccaagttc gcctcttcct tttatggtcc gttccgtgaa
      781 gctgccggaa gcgcattaaa aggcgaccgc aaaagctatc agatgaaccc aatgaaccgt
      841 cgcgaggcga ttcgtgaatc actgctggat gaagcccagg gcgcagactg cctgatggtt
      901 aaacctgctg gagcatacct cgacatcgtg cgtgagctgc gtgaacgtac tgaattgccg
      961 attggcgcgt atcaggtgag cggtgagtac gcgatgatta agttcgccgc gctggcgggt
     1021 gctatagatg aagagaaagt cgtgctcgaa agcttaggtt cgattaagcg tgcgggtgcg
     1081 gatctgattt tcagctactt tgcgctggat ttggctgaga agaagattct gcgttaattt
     1141 ttttcctctc agaaaggggg agttaacctg ttgaaagtac ggtgaacgcc ccctcaccct
     1201 aaccctctcc ccaagggggc gagggaaccg tccgcactcg tatcaccccg cccgataaaa
     1261 cggtttatcc ccaaggatcg tcgcccgatg cataatccgc cgctgtggca ggtaatcggc
     1321 attggcatag tgctgagtca cgcggttatc ccaaatcgca atatcattcg gttgccagcg
     1381 ccaacgcacc tgaaactccg gtttagtgat atgggcgaat aaaaagccta acaaggcttc
     1441 gctctctttc tcgctgacat cgacaattcg cgtagtaaag ccttcattca caaacagcgc
     1501 ctgtttaccg ctcaccggat gcgttcgcac caccggatgt agcaacggag gatttttcgc
     1561 gaccgcctcg cgccagcgtt gatgttcctc ctcggttttg cggtatttgt attccgggaa
     1621 cgatttacgg aaatcatgct ccgcacgcag cccgctcagc agctggcgga agggaacaga
     1681 gagcgcctca taggccgcaa taccgctggt ccagagcgta tcaccgccgg tcgaaggtaa
     1741 ctctttagct gccagaatcg cccctgcggg tggcgtttca ataaatgtca catcggtatg
     1801 ccagttgtcg ttatccggcg gattatcgtt atgggtatcc agcacgatga tctcgtcaac
     1861 cccttcggca tgagggtaaa cagggtgaat atgcagttcg ccaaaacgct gggccagcgc
     1921 gcgttgctgc tgcggcgcaa tagtttgatc gcgcagaaac accacctgat ggcgcagcac
     1981 cgcatggtaa agctgttcaa actgattatc gcttaacggg cgcgtcaggt cggcacccga
     2041 aatttgtgcg ccgatatacg gccccagcgg ggtaatgctc agacgttcac tcattgtact
     2101 tctccatgcc agggcgtcag acggcgctgt aacgcgcgca aacccagttc taaaagaaag
     2161 gcgataatcg caatcaccgc gatccccgcc agcaccacgt cagtcgcgag aaattcaccc
     2221 gctgactgaa ccataaatcc taaaccgcgc gtcgcggcaa tcagctccgc cgctaccagc
     2281 gtagaccagc ccacacccag accaatgcgt aatccggtga ggatttccgg cagcgcgccg
     2341 ggcaaaatga caaaccacag cacctgcgca cggctggcac ccagcgactg ggcggcacga
     2401 atgcgaacct gctgcgcgct tttcaccccc gccagcgccg acatcgccac cggtgcaaaa
     2461 atcgctaaat agatcagtaa gatcttcgag gtttcaccaa taccaaacca gatcaccatc
     2521 agcggcaaat aagccagcgg cggcaccgga cgataaagtt cgattaccgg atccagaatg
     2581 ccgcgcaccg tcgggcttag ccccatggcg atcccgaccg gaataccgat caccaccgcc
     2641 gcgagtagcg ccagcacaat gcgcgtcagg ctggctgcca gatgctgcca cagcgtggcg
     2701 tccataaatc cctgcggtcc ggcaatggtg agcagttttg ccagtacttg ttgcggcggc
     2761 ggcaaaaaca gcgggctaat cagttgcagc gccgccaccg tccaccatac agtgagtaaa
     2821 accgctaacg tgccaatgct taaggtcacc tgacgcgaga gcggccagcg ccatttcagc
     2881 cgccgcgaat gcagtttttc attaatgagc acactcatga gaacgcctcc cgttgctcaa
     2941 atacgcggct taaaacatat tcgcgcatgg cgatgaattg tggatcggac ttgatgctgc
     3001 ggctcgactc tcccgcaaca aagcggcgag caaagttgag cggcagccgc tccagcacgc
     3061 ggccagggcc gggtgaaagc agaaccagtt cagtcgccat aaacaccgct tcttctatat
     3121 cgtgggtaat caacagcacc tgcttgcccg tctcctgcca gagtttcagc agcagggttt
     3181 gcatctggtc gcgggtgaag gcgtccagcg caccaaacgg ctcgtcgagt aataacagct
     3241 ggggattcgc cgccagcgcg cgggcaatcc ccacccgctg acgttgacca ccggaaagct
     3301 gccagatata gcgtttttct gcgccttcca gccccacttt tttcagcatc tggtgcgcga
     3361 tttccagtcg ctgcattttc tctatacctg ccaattgcag gccgaacgcc acgttgtcct
     3421 gtacattgcg ccacggtagc agcccttcat tctgaaaaac caccccgcgc tctgctcccg
     3481 gtccctcaat acgcttaccc gccagttgaa tgctgccatg ctgataaggc acaaaaccgg
     3541 caatcagatt cagcagggtg gttttaccgc agccggacgg ccccagcacc accagtagct
     3601 cgccgctttc cagcgtcagg ttgatatctt ccagtgccgg tttgccgcca taatcggcgt
     3661 aaagatgaga gatttgcagc atctgcgcct ccttttattg cacgaagcgc gaggtaacgt
     3721 actggctgta atcattcgct acagccggga ccttgccctg ctctttcaaa aactgcgcgg
     3781 tgtcgatgat cgctttgttc accggtccgg tcagttctgc cgtttgttgc tgcggcgtca
     3841 gataggtatt ccccttcacc agccccggaa cgtcaccttc cggcacgccg cttaaacgcg
     3901 ccagtttgct gatgttttcc ggctgtttca gccacgcgtc tgggttagca atgtacggtt
     3961 gctgagcatc gatggcgctt ttagcgaacg ctttcacgac ctcaggatgt ttctcggcaa
     4021 aatctttgcg caccacccag acgtccagcg ttggcgcacc ccactgcccg acctgttcag
     4081 aatcggtcag caccttgccg tctttttcca gggcgttaac cgccggtgcc cagacataag
     4141 caccatcaat atctccccgc tgccaggcag cgataatcgc gggcggctgc aggttcacaa
     4201 tctccacttg cccgggttta atgccccagt gtttcagcgc cgccagcagg ctgtagtggg
     4261 tggtagagat aaacggtacg gcgatgcgtt tgccaatcag atcttccggt ttgctgatag
     4321 ttttctttac caccagcgct tcggagttac ccagttttga cgccagcaag aaaacttcaa
     4381 tcggcacctg ttggctggct gcaaccgcta acgggctgga accgaggttg ccgatttgca
     4441 catcgccaga agccagcgcc cgcacgatgc tggctccgct gtcaaactta cgccagtcca
     4501 cggttgctcc gctttcttta gcaaaggtgt tgtcggcctg agccactttc gccggttcgg
     4561 ctgaggtttg atacgcaacg gtgacgttca ccgcctgtgc ctgaaaagcg atgaatgcca
     4621 gtgcggcaag aagtgtgtta cgcgatgaaa ttgccatgat tgtctgctcc cctgtcttgt
     4681 tatgtgagca gtattcagga ataaaaacat tcattaaaag aattagtcgt tatcgcacag
     4741 atgattttat tcttagcaaa aaaacggcaa aggaaagatt aaatatgcaa ttactccgtt
     4801 ctaacaattg ggaaaattac tacacttcat tttagtgatg cataaacaac cacattacga
//
LOCUS       DAZKHF020000292         4814 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2201S31241
            SAMN43267800-rid25106413.denovo.294, whole genome shotgun sequence.
ACCESSION   DAZKHF020000292 DAZKHF020000000
VERSION     DAZKHF020000292.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267800
            Sequence Read Archive: SRR30307263
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4814)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4814)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 4814)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350581.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350581.2
            Genome Coverage       :: 151x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:21:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,652
            CDSs (total)                      :: 5,557
            Genes (coding)                    :: 5,320
            CDSs (with protein)               :: 5,320
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 237
            CDSs (without protein)            :: 237
            Pseudo Genes (ambiguous residues) :: 0 of 237
            Pseudo Genes (frameshifted)       :: 102 of 237
            Pseudo Genes (incomplete)         :: 130 of 237
            Pseudo Genes (internal stop)      :: 52 of 237
            Pseudo Genes (multiple problems)  :: 41 of 237
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4814
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267800-rid25106413.denovo.294"
                     /serotype="O 26:H11"
                     /isolate="2201S31241"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-01"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            complement(<1..596)
                     /locus_tag="ACHOSC_RS21420"
                     /old_locus_tag="pgaptmp_004284"
     CDS             complement(<1..596)
                     /locus_tag="ACHOSC_RS21420"
                     /old_locus_tag="pgaptmp_004284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HGU4550094.1"
                     /translation="MSKEKFERTKPHVNVGTIGHVDHGKTTLTAAITTVLAKTYGGAA
                     RAFDQIDNAPEEKARGITINTSHVEYDTPTRHYAHVDCPGHADYVKNMITGAAQMDGA
                     ILVVAATDGPMPQTREHILLGRQVGVPYIIVFLNKCDMVDDEELLELVEMEVRELLSQ
                     YDFPGDDTPIVRGSALKALEGDAEWEAKILELAGFLDS"
     gene            complement(711..786)
                     /locus_tag="ACHOSC_RS21425"
                     /old_locus_tag="pgaptmp_004285"
     tRNA            complement(711..786)
                     /locus_tag="ACHOSC_RS21425"
                     /old_locus_tag="pgaptmp_004285"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(751..753),aa:Thr,seq:ggt)
     gene            complement(793..867)
                     /locus_tag="ACHOSC_RS21430"
                     /old_locus_tag="pgaptmp_004286"
     tRNA            complement(793..867)
                     /locus_tag="ACHOSC_RS21430"
                     /old_locus_tag="pgaptmp_004286"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(832..834),aa:Gly,seq:tcc)
     gene            complement(983..1067)
                     /locus_tag="ACHOSC_RS21435"
                     /old_locus_tag="pgaptmp_004287"
     tRNA            complement(983..1067)
                     /locus_tag="ACHOSC_RS21435"
                     /old_locus_tag="pgaptmp_004287"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1031..1033),aa:Tyr,seq:gta)
     gene            complement(1076..1151)
                     /locus_tag="ACHOSC_RS21440"
                     /old_locus_tag="pgaptmp_004288"
     tRNA            complement(1076..1151)
                     /locus_tag="ACHOSC_RS21440"
                     /old_locus_tag="pgaptmp_004288"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1116..1118),aa:Thr,seq:tgt)
     gene            1513..2463
                     /gene="coaA"
                     /locus_tag="ACHOSC_RS21445"
                     /old_locus_tag="pgaptmp_004289"
     CDS             1513..2463
                     /gene="coaA"
                     /locus_tag="ACHOSC_RS21445"
                     /old_locus_tag="pgaptmp_004289"
                     /EC_number="2.7.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004594 - pantothenate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I pantothenate kinase"
                     /protein_id="HGU4550095.1"
                     /translation="MSIKEQTLMTPYLQFDRNQWAALRDSVPMTLSEDEIARLKGINE
                     DLSLEEVAEIYLPLSRLLNFYISSNLRRQAVLEQFLGTNGQRIPYIISIAGSVAVGKS
                     TTARVLQALLSRWPEHRRVELITTDGFLHPNQVLKERGLMKKKGFPESYDMHRLVKFV
                     SDLKSGVPNVTAPVYSHLIYDVIPDGDKTVVQPDILILEGLNVLQSGMDYPHDPHHVF
                     VSDFVDFSIYVDAPEDLLQTWYINRFLKFREGAFTDPDSYFHNYAKLTKEEAIKTAMT
                     LWKEINWLNLKQNILPTRERASLILTKSANHAVEEVRLRK"
     gene            complement(2492..3457)
                     /gene="birA"
                     /locus_tag="ACHOSC_RS21450"
                     /old_locus_tag="pgaptmp_004290"
     CDS             complement(2492..3457)
                     /gene="birA"
                     /locus_tag="ACHOSC_RS21450"
                     /old_locus_tag="pgaptmp_004290"
                     /EC_number="6.3.4.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004077 - biotin-[acetyl-CoA-carboxylase]
                     ligase activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional biotin--[acetyl-CoA-carboxylase]
                     ligase/biotin operon repressor BirA"
                     /protein_id="HGU4550096.1"
                     /translation="MKDNTVPLKLIALLANGEFHSGEQLGETLGMSRAAINKHIQTLR
                     DWGVDVFTVPGKGYSLPEPIQLLNAEQILGQLDGGSVAVLPVIDSTNQYLLDRIGELK
                     SGDACVAEYQQAGRGRRGRKWFSPFGANLYLSMFWRLEQGPAAAIGLSLVIGIVMAEV
                     LRKLGADKVRVKWPNDLYLQDRKLAGILVELTGKTGDAAQIVIGAGINMAMRRVEESV
                     VNQGWITLQEAGINLDRNTLAAMLIRELRAALELFEQEGLAPYLSRWEKLDNFINRPV
                     KLIIGDKEIFGISRGIDKQGALLLEQDGIIKPWMGGEISLRSAEK"
     gene            complement(3454..4482)
                     /gene="murB"
                     /locus_tag="ACHOSC_RS21455"
                     /old_locus_tag="pgaptmp_004291"
     CDS             complement(3454..4482)
                     /gene="murB"
                     /locus_tag="ACHOSC_RS21455"
                     /old_locus_tag="pgaptmp_004291"
                     /EC_number="1.3.1.98"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008762 - UDP-N-acetylmuramate
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate dehydrogenase"
                     /protein_id="HGU4550097.1"
                     /translation="MNHSLKPWNTFGIDHNAQHIVCAEDEQQLLNAWQYATAEGQPVL
                     ILGEGSNVLFLEDYRGTVIINRIKGIEIHDEPDAWYLHVGAGENWHRLVKYTLQEGMP
                     GLENLALIPGCVGSSPIQNIGAYGVELQRVCAYVDCVELATGKQVRLTAKECRFGYRD
                     SIFKHEYQDRFAIVAVGLRLPKEWQPVLTYGDLTRLDPTTVTPQQVFNAVCHMRTTKL
                     PDPKVNGNAGSFFKNPVVSAETAKALLAQFPTAPNYPQAGGSVKLAAGWLIDQCQLKG
                     MQMGGAAVHRQQALVLINEDNAKSEDVVQLAHHVRQKVGEKFNVWLEPEVRFIGASGE
                     VSAVETIS"
BASE COUNT         1216 a         1228 c         1095 g         1275 t
ORIGIN      
        1 taagaatcca ggaagccagc cagttccagg attttcgctt cccactctgc gtcgccttcc
       61 agcgctttca gagcagaacc acgaacgatc ggagtgtcgt cgcccgggaa gtcatactga
      121 gacagaagtt cacgaacttc catttcaacc agttccagca gctcttcgtc atcaaccatg
      181 tcgcatttgt tcaggaacac gatgatgtac ggaacgccta cctgacgacc cagcaggatg
      241 tgctcacgag tctgcggcat cgggccgtca gtcgcagcaa ctaccaagat cgcgccgtcc
      301 atctgagcag caccggtgat catgttttta acatagtcgg cgtgccccgg gcagtctacg
      361 tgtgcgtagt gacgggtcgg ggtgtcgtat tcaacgtgag aagtgttgat ggtgatacca
      421 cgagcttttt cttccggcgc gttatcgatc tggtcgaatg cgcgagcagc accgccgtag
      481 gttttagcca gtacggtagt gattgcagcg gtcagcgttg ttttaccatg gtcaacgtgg
      541 ccgatagtac cgacgttaac gtgcggtttt gtacgttcaa acttttcttt agacatcgat
      601 tgtccctcta agacacggat aaatcggtga tatcaccaca tcaaccaggc aacatgcccg
      661 acttgttgaa tgcaataaac agaaggaaaa aacagggagg agaaaagaag tggtgctgat
      721 aggcagattc gaactgccga cctcaccctt accaagggtg cgctctacca actgagctat
      781 atcagcacat cttggagcgg gcagcgggaa tcgaacccgc atcatcagct tggaaggctg
      841 aggtaatagc cattatacga tgcccgcatc ctggaactcg gctacctgat tttcattctg
      901 cactgaatat cgagagaagc tctctttatt cgagccggta agcgaactta tcgtctcggg
      961 ctacgccatc gcgtgccgaa attggtggtg ggggaaggat tcgaaccttc gaagtctgtg
     1021 acggcagatt tacagtctgc tccctttggc cgctcgggaa ccccaccgga cttgatggtg
     1081 ccgactaccg gaatcgaact ggtgacctac tgattacaag tcagttgctc tacctactga
     1141 gctaagtcgg catcaagtag cgcgcattct atggagacat gcgagttcat gcaactaaaa
     1201 aattgcataa tttgttttat tggtcacatt ttatgcgaca cgatgaagaa acagccgaac
     1261 ggagggcaga ttagcacact ttttcaacat cattgtgctc aacaatgcgc tcctgctaaa
     1321 ccataattct ttttatcaga tggaatatct gtcacattgc ttttcaacga tagcttcctg
     1381 gcagagattt tttcttatta ttcctcccca tctggtgtta ccctcctgcc cattaaccca
     1441 ttcaacagaa ctgtgacgcg ccatggcaaa tatcgctttg ccgatagagc tatgaccgcc
     1501 agaaacatgc ttatgagtat aaaagagcaa acgttaatga cgccttacct acagtttgac
     1561 cgcaaccagt gggcagctct gcgtgattcc gtacctatga cgttatcgga agatgagatc
     1621 gcccgtctca aaggtattaa tgaagatctc tcgttagaag aagttgccga gatctattta
     1681 cctttgtcac gtttgctgaa cttctatata agctcgaatc tgcgccgtca ggcagttctg
     1741 gaacagtttc ttggtactaa cgggcaacgc attccttaca ttatcagtat tgctggcagt
     1801 gtcgcggtgg ggaaaagcac aaccgcccgt gtattgcagg cgctattaag ccgttggccg
     1861 gaacatcgtc gtgttgaact gatcactaca gatggcttcc ttcaccctaa tcaggttctg
     1921 aaagaacgtg gtctgatgaa gaagaaaggc ttcccggaat cgtatgatat gcatcgcctg
     1981 gtgaagtttg tttccgatct caaatccggc gtgccaaacg ttacagcccc tgtttactcg
     2041 catcttattt atgatgtgat cccggatgga gataaaacgg ttgttcagcc tgatatttta
     2101 attcttgaag ggttaaatgt cttacagagc gggatggatt atccacacga tccacatcat
     2161 gtatttgttt ctgattttgt cgatttttcg atatatgttg atgcaccgga agacttactt
     2221 cagacgtggt atatcaaccg ttttctgaaa ttccgcgaag gggcttttac cgacccggat
     2281 tcctattttc ataactacgc gaaattaact aaagaagaag cgattaagac tgccatgaca
     2341 ttgtggaaag agatcaactg gctgaactta aagcaaaata ttctacctac tcgtgagcgc
     2401 gccagtttaa tcctgacgaa aagtgctaat catgcggtag aagaggtcag actacgcaaa
     2461 taatttgcag gggagcgaat actccccttt cttatttttc tgcactacgc agggatattt
     2521 caccgcccat ccagggtttt attattccat cctgctcaag taataaagcc ccctgtttat
     2581 ctattccgcg tgaaatgcca aatatttctt tatcaccaat gataagtttc actgggcgat
     2641 taataaaatt atccagcttt tcccagcgcg acagataagg tgccaatcct tcttgttcga
     2701 agagttccaa cgcagcacgt aattcacgta ttagcatggc cgccaacgta ttacgatcga
     2761 gattgatccc cgcttcctgc agcgtgatcc acccctgatt aacgacactc tcttcaacac
     2821 ggcgcattgc catgttgatc ccggctccaa tgactatttg cgccgcatcg ccagttttgc
     2881 cagtcagctc cacaagaatg cctgccagct tgcgatcctg cagatagagg tcattaggcc
     2941 atttgacacg aactttatct gctcccagct tgcgtaatac ttccgccatc acgataccga
     3001 taaccagact taaaccaatc gccgccgccg ggccttgttc cagacgccag aacatcgaca
     3061 aatataagtt tgcgccaaaa ggcgaaaacc atttccgccc ccggcgacca cggccagcct
     3121 gctggtattc tgcaacacag gcatcgcccg atttaagctc tccgatacga tcaagaaggt
     3181 actgattcgt ggagtcaata actggcagca cggctacact accgccatcc agctgaccca
     3241 atatctgttc agcattaagt aactggatgg gctcaggcag gctgtatcct ttacccggaa
     3301 cggtaaagac atcaacgccc cagtcacgca gtgtctgaat gtgtttatta atagccgccc
     3361 ggctcattcc cagcgtttca cccaactgct cgccagagtg aaattcaccg ttcgctaaca
     3421 gggcaatcaa tttcagtggc acggtgttat ccttcatgaa attgtctcca ctgcgctcac
     3481 ttcacctgat gcaccaataa agcggacttc aggctcaagc cagacattaa atttttcacc
     3541 cactttttgt cttacatggt gtgccagttg caccacatct tcgctttttg cattgtcttc
     3601 attaatgaga actaacgcct gttgacggtg caccgcagcc ccacccattt gcatcccttt
     3661 tagctggcac tgatcaataa gccaacctgc tgccagtttt actgaaccac ccgcctgggg
     3721 gtaatttggt gctgttggaa attgcgccag taatgcttta gccgtttcgg cagatacaac
     3781 agggtttttg aagaaactac cggcattgcc attcactttt ggatcaggga gtttggtggt
     3841 gcgcatatga cacaccgcat taaatacttg ctgtggcgtt actgttgtag gatccagacg
     3901 agttaagtca ccatacgtta gtacaggttg ccactctttt ggcagacgca gacctacggc
     3961 tacaatggcg aagcgatcct ggtattcatg tttaaaaata ctgtcgcgat agccaaaacg
     4021 gcactctttg gcagttaagc gcacttgctt gcctgtcgcc agttcaacac aatcaacata
     4081 agcgcaaact cgctgtaatt ctacgccata agcaccaata ttctggatag gtgatgagcc
     4141 gacacaacca ggaattaatg ccagattttc cagaccaggc ataccttcct gcaaagtgta
     4201 ttttaccagg cgatgccagt tttctccggc tcctacatgt aaataccacg catcaggttc
     4261 atcatgaatt tcgatacctt tgatccggtt gatgatcacc gtgccgcgat agtcttccag
     4321 aaaaagtaca ttacttcctt cacccagaat aagaacgggt tgtccttctg cggttgcata
     4381 ctgccaggca ttgagtaatt gttgttcgtc ttcggcacat acaatgtgct gagcattatg
     4441 atcaatgcca aatgtgttcc agggttttaa ggagtggttc atagctgctt tcctgatgca
     4501 aaaacgaggc tagtttaccg tatctgtggg gggatggctt gtagatatga cgacaggaag
     4561 agtttgtaga aacgcaaaaa ggccatccgt caggatggcc ttctgcttaa tttgatgcct
     4621 ggcagtttat ggcgggcgtc ctgcccgcca ccctccgggc cgttgcttcg caacgttcaa
     4681 atccgctccc ggcggatttg tcctactcag gagagcgttc accgacaaac aacagataaa
     4741 acgaaaggcc cagtctttcg actgagcctt tcgttttatt tgatgcctgg cagttcccta
     4801 ctctcgcatg ggga
//
LOCUS       DAZKHF020000293         4733 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2201S31241
            SAMN43267800-rid25106413.denovo.295, whole genome shotgun sequence.
ACCESSION   DAZKHF020000293 DAZKHF020000000
VERSION     DAZKHF020000293.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267800
            Sequence Read Archive: SRR30307263
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4733)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4733)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 4733)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350581.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350581.2
            Genome Coverage       :: 151x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:21:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,652
            CDSs (total)                      :: 5,557
            Genes (coding)                    :: 5,320
            CDSs (with protein)               :: 5,320
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 237
            CDSs (without protein)            :: 237
            Pseudo Genes (ambiguous residues) :: 0 of 237
            Pseudo Genes (frameshifted)       :: 102 of 237
            Pseudo Genes (incomplete)         :: 130 of 237
            Pseudo Genes (internal stop)      :: 52 of 237
            Pseudo Genes (multiple problems)  :: 41 of 237
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4733
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267800-rid25106413.denovo.295"
                     /serotype="O 26:H11"
                     /isolate="2201S31241"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-01"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            <1..846
                     /locus_tag="ACHOSC_RS21460"
                     /old_locus_tag="pgaptmp_004292"
     CDS             <1..846
                     /locus_tag="ACHOSC_RS21460"
                     /old_locus_tag="pgaptmp_004292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415162.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YbeF family transcriptional regulator"
                     /protein_id="HGU4550098.1"
                     /translation="FEAVYVHKGIVNAAKVLNLTPSAISQSIQKLRVIFPDPLFIRKG
                     QGVTPTAFAMHLHEYISQGLESILGALDIEGSYDKQRTITIATTPSVGALVLPVIYRA
                     IKTHYPQLLLRNPPISDAENQLSQFQTDLIIDNMFCTNRTVQHHVLFTDNMVLICREG
                     NPLLSLEDDRETIDNAAHVLLLPEEQNFSGLRQRVQEMFPDRQINFTSYNILTIAALV
                     ANSDMLAIIPSRFYNLFSRCWPLEKLPFPSLNEEQIDFSIHYNKFSLRDPILHGVIDV
                     IRNAF"
     gene            1055..2020
                     /gene="lipA"
                     /locus_tag="ACHOSC_RS21465"
                     /old_locus_tag="pgaptmp_004293"
     CDS             1055..2020
                     /gene="lipA"
                     /locus_tag="ACHOSC_RS21465"
                     /old_locus_tag="pgaptmp_004293"
                     /EC_number="2.8.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012904980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016992 - lipoate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0009107 - lipoate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoyl synthase"
                     /protein_id="HGU4550099.1"
                     /translation="MSKPIVMERGVKYRDADKMALIPVKNVATEREALLRKPEWMKIK
                     LPADSTRIQGIKAAMRKNGLHSVCEEASCPNLAECFNHGTATFMILGAICTRRCPFCD
                     VAHGRPVAPDANEPVKLAQTIADMALRYVVITSVDRDDLRDGGAQHFADCITAIREKS
                     PQIKIETLVPDFRGRMDRALDILTATPPDVFNHNLENVPRIYRQVRPGADYNWSLKLL
                     ERFKEAHPEIPTKSGLMVGLGETNEEIIEVMRDLRRHGVTMLTLGQYLQPSRHHLPVQ
                     RYVSPDEFDEMKAEALAMGFTHAACGPFVRSSYHADLQAKGMEVK"
     gene            complement(2120..2323)
                     /gene="tatE"
                     /locus_tag="ACHOSC_RS21470"
                     /old_locus_tag="pgaptmp_004294"
     CDS             complement(2120..2323)
                     /gene="tatE"
                     /locus_tag="ACHOSC_RS21470"
                     /old_locus_tag="pgaptmp_004294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocase subunit TatE"
                     /protein_id="HGU4550100.1"
                     /translation="MGEISITKLLVVAALVVLLFGTKKLRTLGGDLGAAIKGFKKAMN
                     DDDAAAKKGADVDLQAEKLSHKE"
     gene            complement(2452..3240)
                     /locus_tag="ACHOSC_RS21475"
                     /old_locus_tag="pgaptmp_004295"
     CDS             complement(2452..3240)
                     /locus_tag="ACHOSC_RS21475"
                     /old_locus_tag="pgaptmp_004295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deaminated glutathione amidase"
                     /protein_id="HGU4550101.1"
                     /translation="MLVAAGQFAVTSVWEKNAEICASLMAQAAENDVSLFVLPEALLA
                     RDDHDADLSVKSAQLLEGEFLGRLRRESKRNMMTTILTIHVPSTPGRAWNMLVALQAG
                     NIVARYAKLHLYDAFAIQESRRVDAGNEIAPLLEVEGMKVGLMTCYDLRFPELALAQA
                     LQGAEILVLPAAWVRGPLKEHHWSTLLAARALDTTCYMVAAGECGNKNIGQSRIIDPF
                     GVTIAAASEMPALIMAEVTPERVRQVRAQLPVLNNRRFAPPQLL"
     gene            3333..3716
                     /gene="crcB"
                     /locus_tag="ACHOSC_RS21480"
                     /old_locus_tag="pgaptmp_004296"
     CDS             3333..3716
                     /gene="crcB"
                     /locus_tag="ACHOSC_RS21480"
                     /old_locus_tag="pgaptmp_004296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fluoride efflux transporter CrcB"
                     /protein_id="HGU4550102.1"
                     /translation="MLQLLLAVFIGGGTGSVARWLLSMRFNPLHQAIPLGTLAANLIG
                     AFIIGMGFAWFSRMTNIDPVWKVLITTGFCGGLTTFSTFSAEVVFLLQEGRFGWALLN
                     VFVNLLGSFAMTALAFWLFSASTAH"
     gene            complement(3771..3980)
                     /gene="cspE"
                     /locus_tag="ACHOSC_RS21485"
                     /old_locus_tag="pgaptmp_004297"
                     /gene_synonym="gicA"
                     /gene_synonym="msmC"
     CDS             complement(3771..3980)
                     /gene="cspE"
                     /locus_tag="ACHOSC_RS21485"
                     /old_locus_tag="pgaptmp_004297"
                     /gene_synonym="gicA"
                     /gene_synonym="msmC"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator/RNA stability
                     regulator CspE"
                     /protein_id="HGU4550103.1"
                     /translation="MSKIKGNVKWFNESKGFGFITPEDGSKDVFVHFSAIQTNGFKTL
                     AEGQRVEFEITNGAKGPSAANVIAL"
     gene            complement(4155..4715)
                     /gene="pagP"
                     /locus_tag="ACHOSC_RS21490"
                     /old_locus_tag="pgaptmp_004298"
     CDS             complement(4155..4715)
                     /gene="pagP"
                     /locus_tag="ACHOSC_RS21490"
                     /old_locus_tag="pgaptmp_004298"
                     /EC_number="2.3.1.251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid IV(A) palmitoyltransferase PagP"
                     /protein_id="HGU4550104.1"
                     /translation="MNVSKYVAIFSFVFIQLISVGKVFANADEWMTTFRENIAQTWQQ
                     PEHYDLYIPAITWHARFAYDKEKTDRYNERPWGGGFGLSRWDEKGNWHGLYAMAFKDS
                     WNKWEPIAGYGWESTWRPLADENFHLGLGFTAGVTARDNWNYIPLPVLLPLASVGYGP
                     VTFQMTYIPGTYNNGNVYFAWMRFQF"
BASE COUNT         1241 a         1206 c         1075 g         1211 t
ORIGIN      
        1 tttgaagctg tatatgtaca taaagggatc gttaatgcag cgaaagtgct taatctgacc
       61 ccctcggcaa tcagtcagtc tattcagaaa ctgcgcgtta tattccctga cccattgttt
      121 attcgcaaag gccagggtgt cactcctacc gcatttgcga tgcatctaca tgagtatatc
      181 agtcagggcc ttgagtccat tcttggcgcg ctggatatcg aaggaagcta tgataagcaa
      241 cgaacgataa ctattgcgac cactccctcg gtcggagccc tggtccttcc tgtcatctat
      301 cgggcgatta aaactcacta tccgcagctt ttactgcgca acccgcccat cagcgacgcg
      361 gaaaaccaac tcagtcagtt tcaaaccgat ctcatcatcg ataacatgtt ttgcaccaat
      421 cgtacggtgc aacatcatgt tctgttcacc gacaatatgg tgttaatttg ccgcgaggga
      481 aatccactac tctctttaga agatgacaga gagactatcg acaacgctgc gcatgtactt
      541 ctgttaccgg aagagcaaaa tttcagcggt ctgcggcaga gagttcaaga aatgtttccg
      601 gaccggcaaa tcaatttcac cagctacaac attttgacaa tcgctgcact ggttgccaac
      661 agtgacatgt tagcgattat tcccagccgt ttttataacc tgtttagccg ctgctggccg
      721 ctggaaaaac tgccttttcc gtccttaaat gaggagcaaa tagatttctc tatccactac
      781 aacaaattca gcctgcgtga tcccatcctg cacggagtaa tagatgttat ccgtaatgca
      841 ttttgaaaaa gtattgttac agccagatca cagcgcagaa cgataaaatg gcacaatcca
      901 acaacaattt tacattttcg cgaccgcttt ggctgctttc aggtccgttt caatgatata
      961 ctgccagtcg ttaattcaaa aatagttgat aattacaaca atctattgaa ttgaaacgct
     1021 ttccttcgta attcgcaact ggaacacgca cgctatgagt aaacccattg tgatggaacg
     1081 cggtgttaaa taccgcgatg ccgataagat ggcccttatc ccggttaaaa acgtggcaac
     1141 agagcgcgaa gccctgctgc gcaagccgga atggatgaaa atcaagcttc cagcggactc
     1201 tacacgtatc cagggcatca aagccgcaat gcgcaaaaat ggcctgcatt ctgtctgcga
     1261 ggaagcctcc tgccctaacc tggcggaatg cttcaaccac ggcacagcaa cgtttatgat
     1321 cctcggcgct atttgtaccc gccgttgtcc gttctgtgat gttgcccacg gtcgcccggt
     1381 agctcctgat gccaatgaac cagtgaaact ggcgcagacc attgccgata tggcgctgcg
     1441 ttatgtggtt atcacctccg ttgaccgtga tgacctgcgc gatggcggtg cccagcactt
     1501 tgcggattgc attactgcca ttcgggaaaa aagcccgcaa atcaaaattg aaactctggt
     1561 gccggatttc cgtggtcgta tggatcgtgc tctggatatt ctgactgcaa cgccaccaga
     1621 tgtgttcaac cataacctgg aaaacgtacc gcgtatttac cgtcaggtac ggcctggtgc
     1681 agattacaac tggtcgctga agctgctgga acgctttaaa gaagcgcatc cggaaatccc
     1741 gaccaagtct ggtctgatgg tgggactggg tgaaaccaat gaagaaatta ttgaggtaat
     1801 gcgcgacctg cgccgtcatg gtgtgacgat gttaacgctg gggcaatatt tgcagccaag
     1861 ccgccatcac ctgccggttc aacgttacgt tagcccggat gagttcgacg aaatgaaagc
     1921 cgaagcgctg gcgatgggct ttacccatgc tgcatgcggt ccgtttgtcc gctcttctta
     1981 ccacgccgat ttgcaggcga aagggatgga agttaagtaa tcctgtaata atacttacat
     2041 ctgtctgata aaaaacccgc tttttgaagc gggttttttg tatcgaacaa gatattgagg
     2101 gagcgtcctg ctcgccacgt cactctttat gagagagctt ttcagcctga agatcaacgt
     2161 ctgcgccttt tttcgccgca gcatcgtcat cattcatcgc cttcttgaac cctttaatgg
     2221 ccgctccaag gtctccgccc agcgtacgta acttcttagt cccaaacagc agaacgacca
     2281 gcgccgcaac tactagcagt ttggtaatac taatctcacc catagatacc ttcttgacat
     2341 ataaccggca acagaacgcg ccttaaaaac cagagaatat taacggtcat tcgacgcgcg
     2401 cacacaatag caatctgtaa caaggtgaat caagcgccga gtaaaaaaac atcataataa
     2461 ttgcggcggc gcaaagcgac ggttgtttaa gacgggcagt tgcgcgcgca cctgacgcac
     2521 acgttcgggc gtcacttccg ccataatgag tgcaggcatt tctgacgctg ccgcaatggt
     2581 gacgccaaag ggatctataa tccggctttg accgatattt ttgttcccgc actcccccgc
     2641 cgccaccata taacaggtgg tatccagcgc acgagcggca agcaacgttg accaatgatg
     2701 ctctttgagc ggcccgcgaa cccaggcggc aggaagtacc aggatttcag ctccctgtaa
     2761 tgcctgtgcc agcgccagct ctggaaagcg taagtcataa caggtcatca gaccgacctt
     2821 catcccctcc acctcgagta acggagcgat ttcattacca gcatcaacac ggcgtgattc
     2881 ctgaatggca aatgcatcat agagatgcaa tttggcataa cgggcgacga tgtttcctgc
     2941 ctgtagcgcc accagcatat tccatgcgcg ccccggcgtt gaaggaacat gaatcgtcag
     3001 aattgtcgtc atcatgttac gtttactttc tcgccgtaaa cgtccgagga attcgccttc
     3061 cagcagctgt gctgatttaa ccgatagatc tgcatcatga tcatcgcgcg ccagcaatgc
     3121 ttccggcagg acgaacagcg atacgtcgtt ttccgccgcc tgcgccatca acgaggcaca
     3181 aatctcagcg ttcttttccc acacagatgt aacagcaaac tgtcctgcgg caaccagcat
     3241 tgccacctcc tgtcagagtc gtaaaattcg ccaacgacga ttattcggcg ttacacttat
     3301 cactcataca aatcaaatag caggattttg cagtgttaca acttctttta gcagttttta
     3361 ttggcggtgg tacgggaagc gtggcgagat ggctgttaag tatgcgattt aacccgctgc
     3421 atcaggcgat tccgttgggg acgctggcag caaacctgat tggggcattc atcataggaa
     3481 tgggattcgc ctggttcagc aggatgacga acattgatcc agtgtggaaa gtattaatca
     3541 ccaccggatt ttgtggcggt ctaacaacct tctcaacatt ttcggcagaa gtggtgtttt
     3601 tgttacaaga gggccgcttt ggctgggcgt tactgaacgt tttcgtcaac cttctggggt
     3661 cttttgccat gacagcactg gctttctggc tgttttcggc ctcaaccgca cactaaagac
     3721 caaaaaaaaa cccgctgatt aagcgggttt tgaattcttg ctgacgtatc ttacagagcg
     3781 attacgtttg cagcagaagg gcctttggca ccgttagtga tttcgaactc tacgcgctga
     3841 ccttcagcaa gagttttaaa accattagtc tggattgcag agaagtgtac gaacacgtct
     3901 ttgctgccgt cttccggagt aatgaaaccg aatcctttgg actcattaaa ccacttaacg
     3961 ttacctttaa tcttagacat caaaattacc tttacatgaa aaatagacac aaatgctgtg
     4021 tcggttacca gtacaccaat tgtggtacgc tttgtccagt gcaactttac taaaaagtga
     4081 taaaagtcgc gttatttttg atgttactta ttttagctat tgattttaaa gaagttacta
     4141 aaacttcatt tgtctcaaaa ctgaaagcgc atccaggcaa agtacacatt gccattgttg
     4201 taggtacccg gaatgtaggt catctgaaaa gtcactgggc cataacccac ggaggccaat
     4261 ggcagtagaa ccgggagagg gatgtaattc cagttatcgc gtgccgttac gccagcggtg
     4321 aatcccagac ctaaatgaaa attttcatcc gccagcggtc gccaggtact ttcccatccg
     4381 tatccggcaa tcggttccca tttgttccac gagtccttaa atgccatggc atacaggcca
     4441 tgccagtttc ctttttcatc ccaacgcgac aggccaaaac cgccacccca cggtcgctcg
     4501 ttatagcgat cggttttttc tttgtcgtaa gcgaaacgtg catgccaggt gatggcagga
     4561 atatataaat cataatgttc aggctgttgc caggtttgtg caatattttc tctaaacgtt
     4621 gtcatccact catctgcgtt agcaaaaact ttaccaacgc tgattaactg aataaaaaca
     4681 aaggaaaaga tagcgacata tttactcacg ttcatttgtg accataaaac att
//
LOCUS       DAZKHF020000294         4705 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2201S31241
            SAMN43267800-rid25106413.denovo.296, whole genome shotgun sequence.
ACCESSION   DAZKHF020000294 DAZKHF020000000
VERSION     DAZKHF020000294.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267800
            Sequence Read Archive: SRR30307263
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4705)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4705)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 4705)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350581.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350581.2
            Genome Coverage       :: 151x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:21:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,652
            CDSs (total)                      :: 5,557
            Genes (coding)                    :: 5,320
            CDSs (with protein)               :: 5,320
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 237
            CDSs (without protein)            :: 237
            Pseudo Genes (ambiguous residues) :: 0 of 237
            Pseudo Genes (frameshifted)       :: 102 of 237
            Pseudo Genes (incomplete)         :: 130 of 237
            Pseudo Genes (internal stop)      :: 52 of 237
            Pseudo Genes (multiple problems)  :: 41 of 237
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4705
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267800-rid25106413.denovo.296"
                     /serotype="O 26:H11"
                     /isolate="2201S31241"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-01"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            complement(39..800)
                     /gene="kduD"
                     /locus_tag="ACHOSC_RS21495"
                     /old_locus_tag="pgaptmp_004299"
     CDS             complement(39..800)
                     /gene="kduD"
                     /locus_tag="ACHOSC_RS21495"
                     /old_locus_tag="pgaptmp_004299"
                     /EC_number="1.1.1.127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0000272 - polysaccharide catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydro-3-deoxy-D-gluconate 5-dehydrogenase
                     KduD"
                     /protein_id="HGU4550105.1"
                     /translation="MILSAFSLEGKVAVVTGCDTGLGQGMALGLAQAGCDIVGINIVE
                     PTETIEQVTALGRRFLSLTADLRKIDGIPGLLDRAVAEFGHIDILVNNAGLIRREDAL
                     EFSEKDWDDVMNLNIKSVFFMSQAAAKHFIAQGNGGKIINIASMLSFQGGIRVPSYTA
                     SKSGVMGVTRLMANEWAKHNINVNAIAPGYMATNNTQQLRADEQRSAEILDRIPAGRW
                     GLPSDLMGPIVFLASSASDYVNGYTIAVDGGWLAR"
     gene            complement(830..1666)
                     /gene="kduI"
                     /locus_tag="ACHOSC_RS21500"
                     /old_locus_tag="pgaptmp_004300"
     CDS             complement(830..1666)
                     /gene="kduI"
                     /locus_tag="ACHOSC_RS21500"
                     /old_locus_tag="pgaptmp_004300"
                     /EC_number="5.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008697 -
                     4-deoxy-L-threo-5-hexosulose-uronate ketol-isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0045490 - pectin catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-dehydro-4-deoxy-D-glucuronate isomerase"
                     /protein_id="HGU4550106.1"
                     /translation="MDVRQSIHSAHAKTLDTQGLRNEFLVEEVFVADEYTMVYSHIDR
                     IIVGGIMPITKTVSVGGEVGKQLGVSYFLERRELGVINIGGAGTITVDGQCYEIGHRD
                     ALYVGKGAKEVVFASIDTATPAKFYYNCAPAHTTYPTKKVTPDEVSPVTLGDNLTSNR
                     RTINKYFVPDVLETCQLSMGLTELAPGNLWNTMPCHTHERRMEVYFYFNMDDDACVFH
                     MMGQPQETRHIVMHNEQAVISPSWSIHSGVGTKAYTFIWGMVGENQVFDDMDHVAVKD
                     LR"
     gene            complement(1953..3134)
                     /gene="yqeF"
                     /locus_tag="ACHOSC_RS21505"
                     /old_locus_tag="pgaptmp_004301"
     CDS             complement(1953..3134)
                     /gene="yqeF"
                     /locus_tag="ACHOSC_RS21505"
                     /old_locus_tag="pgaptmp_004301"
                     /EC_number="2.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708633.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acetyltransferase"
                     /protein_id="HGU4550107.1"
                     /translation="MKDVVIVGALRTPIGCFRGALAGHSAVELGSLVVKALIERTGVP
                     AYAVDEVILGQVLTAGAGQNPARQSAIKGGLPNSVSAITINDVCGSGLKALHLATQAI
                     QCGEADIVIAGGQENMSRAPHVLTDSRTGAQLGNSQLVDSLVHDGLWDAFNDYHIGVT
                     AENLAREYGISRQLQDAYALSSQQKARAAIDAGRFKDEIVPVMIQSNGQTLVVDTDEQ
                     PRTDASAEGLARLNPSFDSLGSVTAGNASSINDGAAAVMMMSEAKARALNLPVLARIR
                     AFASVGVDPALMGIAPVYATRRCLERVGWQLADVDLIEANEAFAAQALSVGKMLEWDE
                     RRVNVNGGAIALGHPIGASGCRILVSLVHEMVKRNARKGLATLCIGGGQGVALTIERD
                     E"
     gene            3389..4618
                     /gene="yqeG"
                     /locus_tag="ACHOSC_RS21510"
                     /old_locus_tag="pgaptmp_004302"
     CDS             3389..4618
                     /gene="yqeG"
                     /locus_tag="ACHOSC_RS21510"
                     /old_locus_tag="pgaptmp_004302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HGU4550108.1"
                     /translation="MSNIWSKEETLWSFALYGTAVGAGTLFLPIQLGSAGAVVLFITA
                     LVAWPLTYWPHKALCQFILSSKTSAGEGITGAVTHYYGKKIGNLITTLYFIAFFVVVL
                     IYAVAITNSLTEQLAKHMVIDLRIRMLVSLGVVLILNLIFLMGRHATIRVMGFLVFPL
                     IAYFLFLSIYLVGSWQPDLLTTQVEFNQNTLHQIWISIPVMVFAFSHTPIISTFAIDR
                     REKYGEHAMDKCKKIMKVAYLIICISVLFFVFSCLLSIPPSYIEAAKEEGVTILSALS
                     MLPNAPAWLSISGIIVAVVAMSKSFLGTYFGVIEGATEVVKTTLQQVGVKKSRAFNRA
                     LSIMLVSLITFIVCCINPNAISMIYAISGPLIAMILFIMPTLSTYLIPALKPWRSIGN
                     LITLIVGILCVSVMFFS"
BASE COUNT         1215 a         1213 c         1038 g         1239 t
ORIGIN      
        1 ctgccatgcg gcagggtcat aaaagtaaga agaatgaatt aacgcgccag ccaaccgcca
       61 tccacggcaa tggtataacc attcacataa tctgaagcgc tggaggcaag gaacactatc
      121 ggccccatca ggtcactcgg cagtccccaa cgaccagctg gaatgcggtc gagaatttcc
      181 gcgctacgtt gttcatctgc ccgtagttgt tgagtattgt tggtcgccat gtaacccgga
      241 gctatcgcat taacattaat gttgtgttta gcccattcgt tcgccatcaa tcgcgtcaca
      301 cccatcacgc cgctttttga tgcggtataa gaaggcacac ggatcccgcc ctggaaggag
      361 agcattgacg cgatattgat aatcttgccg ccattgcctt gcgcgataaa gtgtttcgcc
      421 gctgcctgag acatgaagaa tacgctcttg atattcaggt tcatgacatc gtcccagtcc
      481 ttttcgctga actcgagagc atcttcgcgg cgaatcaatc cggcgttatt caccaggata
      541 tcaatatgac caaactccgc taccgcgcga tccagcagtc caggaatgcc atcaatcttt
      601 cgcagatcgg cggtcaggct taaaaaacga cgccccagcg ccgtgacctg ctcgatggtt
      661 tcagtcggtt caacgatgtt aatgccaaca atgtcacagc ccgcttgcgc cagccccaac
      721 gccatcccct ggcccagccc agtatcacaa ccagtgacga ccgcaacttt accttcgaga
      781 gaaaatgcac ttaaaatcat aacaatacct tattcgttta tgcccacaac tagcgcaaat
      841 ctttaacggc cacatggtcc atatcatcaa agacctggtt ttcaccgacc atgccccaga
      901 taaaggtata agctttggtt ccgacgccgg aatgaatcga ccagctcggg gagatcaccg
      961 cctgctcgtt atgcatcaca atgtgacgcg tttcttgcgg ctgccccatc atgtggaaaa
     1021 cgcaggcgtc atcatccata ttgaaataga aataaacttc catccggcgc tcgtgagtgt
     1081 gacacggcat ggtgttccac aagttacctg gagccagctc cgtcagcccc atactcaatt
     1141 ggcaggtttc cagcacatcc gggacaaaat atttgttaat tgtgcgacgg ttactggtga
     1201 ggttatcgcc taacgtgact ggagatactt cgtccggtgt gacttttttg gtgggatacg
     1261 tcgtatgcgc gggtgcgcaa ttgtaataaa actttgccgg agtggcggta tcaatactgg
     1321 caaagacaac ttcttttgca cctttaccca catacagggc gtcgcggtga ccgatttcat
     1381 agcattggcc atcgacagta atcgtaccgg caccgccaat attgataaca cctaactcgc
     1441 gacgttcaag gaaatagctt acgccgagtt gtttaccaac ttccccgcca acggaaaccg
     1501 ttttagttat cggcataatg ccgccaacaa taattcggtc aatgtggctg taaaccatgg
     1561 tgtactcatc ggcgacaaat acctcttcaa ccaaaaattc attgcgcagc ccttgggtat
     1621 ccagcgtttt tgcgtgcgca ctgtggatgc tctgtcttac gtccacatca acctccgata
     1681 atcagtgaag cgtgaaaata gaaaacgata gtgaaacact gtttcgtttt ctctataaag
     1741 ggatgatatg cccatgggct agggttttca attaaaaata aaacaatgtt tcatttttaa
     1801 gttaagaatt aaaaaaagtg cagtgtcgat cacgaatatg tcatgagaat ggaaaaatga
     1861 gggagcccag aaaatgtatg tcgacttgcc agccatcatt attcaacaga ttaatgacgg
     1921 cagatattta tcggtaagga ggaaatgcag agctactcgt cacgttcaat ggtcaatgcc
     1981 acgccctggc ccccgccgat acaaagcgtt gccagccctt tgcgggcatt acgtttcacc
     2041 atttcatgaa ccagagaaac caggattcgg caaccggaag cgcctatcgg gtgaccgagt
     2101 gcgatcgcgc caccattgac attgacccga cgctcatccc actcaagcat cttgccaacc
     2161 gaaagcgcct gtgcagcaaa cgcttcatta gcctcgataa gatcgacatc agccaactgc
     2221 cagcctacac gctccaggca acggcgggtc gcatacaccg gcgcaattcc catcaatgcc
     2281 ggatctacac caacgctggc aaatgcgcgg atgcgggcca gcacgggtaa attcaacgct
     2341 cgtgctttgg cttcgctcat catcattacc gcagctgcgc catcgtttat ggatgatgca
     2401 ttccccgcgg tcacagaacc gagactatca aatgaaggat ttaaacgggc taaaccttct
     2461 gcactggcgt cagtgcgtgg ctgttcatcg gtatcaacaa ccaacgtctg accgttactt
     2521 tggatcatta ccgggacgat ctcatcttta aatcgtccgg cgtcaatcgc cgctcgcgct
     2581 ttttgttgcg agctaagtgc gtaagcatcc tgcaactgac ggctgatgcc atattcgcga
     2641 gccagatttt cggcggtgac accaatatga taatcattga aggcatccca caacccatca
     2701 tgcaccagac tgtcaaccaa ctggctattg ccaagctgtg caccggtgcg gctatcagtc
     2761 agaacatgtg gtgcgcggct catgttttcc tggccaccgg cgatgacaat atcagcctcg
     2821 ccacactgta tcgcctgagt agccagatgc agtgctttaa gcccagaacc gcaaacgtca
     2881 ttaatagtga ttgcagaaac gctattagga agaccacctt taatagccga ttgccttgcc
     2941 ggattctgcc ctgcccctgc agtcaacacc tgaccaagaa ttacttcatc caccgcatat
     3001 gcaggaacgc cggtacgttc tattaacgct ttcacgacca gactaccaag ttccacggcg
     3061 gaatgacccg ctaacgcacc gcgaaagcag ccgataggtg tccgtaacgc cccgacaatc
     3121 acaacgtctt tcatcacaac ctcgcaccaa ttaacagtgc aatagtaaat gattgttaat
     3181 tacagttatc ttaattgttt aaaaaaagtg atttttatca caaaggaaat atgcctgagc
     3241 agtagtcaga gtcataactg gcacgtaagg tttgcaacca ctaacccacc aatagagggg
     3301 tagatagggc gttaatctcc catacttaac ctggtttatg gtaaattgcc ctccattttg
     3361 tttaatttgt agatgatacg ttcagataat gtctaatatt tggtctaaag aagaaactct
     3421 gtggagtttc gcgctctacg gcacagccgt tggtgcaggc acgctcttcc ttcctattca
     3481 gttaggttcg gcaggggctg tggtcctgtt tattactgct ctggtcgcct ggcctttaac
     3541 atattggcca cataaagcct tatgccagtt catcctctca tcgaaaacat cagcaggtga
     3601 agggataacg ggcgcagtaa cacactacta tggcaagaag attggtaatc tgattaccac
     3661 gctgtacttc atcgcctttt ttgtcgtcgt gttgatatat gcagtggcaa ttaccaactc
     3721 acttacggaa cagctggcaa agcatatggt tattgatctt cgcatccgta tgttggtgag
     3781 tctgggtgtt gtattaatcc tgaatctcat ttttctgatg ggacgccatg caaccattcg
     3841 ggtaatgggt tttttggtat tcccattgat tgcctatttc ttatttcttt ccatttacct
     3901 tgtcggtagt tggcaacctg atctattaac gacccaagta gagttcaatc agaataccct
     3961 tcaccagata tggatatcga ttcccgtgat ggttttcgcc tttagccata cgcccattat
     4021 ttctacgttt gccatagaca gacgtgaaaa atatggcgaa cacgctatgg ataaatgcaa
     4081 aaaaattatg aaagtcgctt atctcatcat ctgcataagt gtactgttct ttgtctttag
     4141 ctgcctgctt tctattccac cttcgtatat tgaagcggct aaagaagaag gggtcaccat
     4201 tttatcggcg ctttctatgc tgccgaacgc cccagcatgg ctgtcaattt ccgggattat
     4261 tgtcgcagta gttgcgatgt cgaaatcatt cctgggtacg tactttggcg ttattgaagg
     4321 tgccacggag gtcgtcaaaa caacactaca gcaggttggt gtaaagaaaa gtcgtgcatt
     4381 taaccgcgca ctatcaatta tgttggtatc gctgatcacc ttcattgttt gttgcattaa
     4441 cccgaacgcg atttcgatga tttacgcgat cagcggcccg ctcattgcca tgatactttt
     4501 catcatgcct acgttgtcaa cgtatctgat cccggcgctt aaaccctggc gttccatcgg
     4561 aaatctgatt acgctgatcg tgggtatcct gtgcgtatcg gtaatgttct ttagctgata
     4621 tccattgtga atggttcaaa attgctacaa ttttgaacca ttccagtttt cataatgttc
     4681 attcgttatt attccgcact atcac
//
LOCUS       DAZKHF020000295         4657 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2201S31241
            SAMN43267800-rid25106413.denovo.297, whole genome shotgun sequence.
ACCESSION   DAZKHF020000295 DAZKHF020000000
VERSION     DAZKHF020000295.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267800
            Sequence Read Archive: SRR30307263
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4657)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4657)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 4657)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350581.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350581.2
            Genome Coverage       :: 151x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:21:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,652
            CDSs (total)                      :: 5,557
            Genes (coding)                    :: 5,320
            CDSs (with protein)               :: 5,320
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 237
            CDSs (without protein)            :: 237
            Pseudo Genes (ambiguous residues) :: 0 of 237
            Pseudo Genes (frameshifted)       :: 102 of 237
            Pseudo Genes (incomplete)         :: 130 of 237
            Pseudo Genes (internal stop)      :: 52 of 237
            Pseudo Genes (multiple problems)  :: 41 of 237
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4657
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267800-rid25106413.denovo.297"
                     /serotype="O 26:H11"
                     /isolate="2201S31241"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-01"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            <1..753
                     /gene="paa"
                     /locus_tag="ACHOSC_RS21515"
                     /old_locus_tag="pgaptmp_004303"
     CDS             <1..753
                     /gene="paa"
                     /locus_tag="ACHOSC_RS21515"
                     /old_locus_tag="pgaptmp_004303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AcfC family adhesin Paa"
                     /protein_id="HGU4550109.1"
                     /translation="NIMAGFLIFLSSAAYADINLYGPGGPHTALLDAAKLYAEKTGII
                     VNVHYGPQNKWNEDAKKNADILFGASEQSALAIIRDHKDSFSEKDIQPLYLRKSILLV
                     KKGNPKNIRSIDDLTRPGIGVIVNDGGGTSNTSGTGVWEDIAGRKGNIETVAAIRKNI
                     ILYAPNSGTARKALENQPGADVWITWADWAASNPEIGDVVEIAPDYVIWRDMNITVRQ
                     DANDETRRFAEWLQTDEAAPAFKKYGWTRKGT"
     gene            1089..1244
                     /gene="hokD"
                     /locus_tag="ACHOSC_RS21520"
                     /old_locus_tag="pgaptmp_004304"
     CDS             1089..1244
                     /gene="hokD"
                     /locus_tag="ACHOSC_RS21520"
                     /old_locus_tag="pgaptmp_004304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin HokD"
                     /protein_id="HGU4550110.1"
                     /translation="MKQQKAMLIALIVICLTVIVTALVTRKDLCEVRIRTGQTEVAVF
                     TAYEPEE"
     gene            1465..1722
                     /locus_tag="ACHOSC_RS21525"
                     /old_locus_tag="pgaptmp_004305"
     CDS             1465..1722
                     /locus_tag="ACHOSC_RS21525"
                     /old_locus_tag="pgaptmp_004305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000687433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4550111.1"
                     /translation="MRIEELREIFSEDGLYTVRVEKGAIVSHCRIKCLQSQQRKSGAA
                     LIHFVDGLVTDGFILRANEFVTSLPSLKEAGIKAGFSAFED"
     gene            1792..2070
                     /locus_tag="ACHOSC_RS21530"
                     /old_locus_tag="pgaptmp_004306"
     CDS             1792..2070
                     /locus_tag="ACHOSC_RS21530"
                     /old_locus_tag="pgaptmp_004306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4550112.1"
                     /translation="MAQNSRLHNSDNSAVFASRHGRRSHAFKSDWFRHAPCTEEQAEW
                     LIQNYRRRGYEFRKALSLDYRHWIIYVRLPYSERPPRPSRTFQQRIWR"
     gene            2072..3127
                     /locus_tag="ACHOSC_RS21535"
                     /old_locus_tag="pgaptmp_004307"
     CDS             2072..3127
                     /locus_tag="ACHOSC_RS21535"
                     /old_locus_tag="pgaptmp_004307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001265086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF968 domain-containing protein"
                     /protein_id="HGU4550113.1"
                     /translation="MRVLLRPVPVPELGLVVLKPGRESMQVFHNPRVLVEPEPKSMRG
                     LPSGIVPAVRQPLAEDKSLLPFFSNERVIRAVGGAGALSDWLLRHVKSCQWPHGDYHH
                     SETVIHRYGTGAMVLCWHCDNQLRDQTSESLEQLAHQNLSAWMIDVIGHAISGTQERE
                     LSLAELSWWAVRNQVADALPEAVLRRSLGLRAEKIRSMYRESDIVPGEQTATIILKQR
                     TKNLAPLPHAHQQNPPQEKTVVSIAVDPESPAQYLQRQKPQREEMPVYTRWVKTQKCM
                     TCGNQADDPHHIIGHGLGGMGTKADDLFVIPLCRKCHSELHAGVKDFEEKHGSQLLLL
                     IRFLMHARNSGVLKWKA"
     gene            3128..3493
                     /locus_tag="ACHOSC_RS21540"
                     /old_locus_tag="pgaptmp_004308"
     CDS             3128..3493
                     /locus_tag="ACHOSC_RS21540"
                     /old_locus_tag="pgaptmp_004308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RusA family crossover junction
                     endodeoxyribonuclease"
                     /protein_id="HGU4550114.1"
                     /translation="MTERIEFVLPYPPTVNTYWRRHGNTYFISEAGKRYRRDVALIVR
                     QQRLKLNLSGRLVIKIIAEPPDKRRRDLDNILKAPLDVLTHAGLLIDDEQFDEINIVR
                     GQLVPGGRLGIKITELECA"
     gene            3490..4179
                     /locus_tag="ACHOSC_RS21545"
                     /old_locus_tag="pgaptmp_004309"
     CDS             3490..4179
                     /locus_tag="ACHOSC_RS21545"
                     /old_locus_tag="pgaptmp_004309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriophage antitermination protein Q"
                     /protein_id="HGU4550115.1"
                     /translation="MNNHYLQFVREQLIIATADLSGATKGQLEAWQENAMFDTGRYRR
                     KKIRYRDEVTGKMITRDNPPIPGKQSLAKVTSIPLVSPVEFSTSSWRRAVLSLEEHHK
                     AWLLWCYSGSICWEYQIAITQWAWNEFNTQSGTRKIAGKTQERLKKLIWLAAQAVKAE
                     LFGGEGYEYQELALLAGVTTKNWSKTFTRHWVAMKHIFHRLDSEALLFVMRTRSKQKA
                     AFSKQSVAKVD"
     gene            4375..4450
                     /locus_tag="ACHOSC_RS21550"
                     /old_locus_tag="pgaptmp_004310"
     tRNA            4375..4450
                     /locus_tag="ACHOSC_RS21550"
                     /old_locus_tag="pgaptmp_004310"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:4408..4410,aa:Ile,seq:cat)
     gene            4541..4614
                     /locus_tag="ACHOSC_RS21555"
                     /old_locus_tag="pgaptmp_004311"
     tRNA            4541..4614
                     /locus_tag="ACHOSC_RS21555"
                     /old_locus_tag="pgaptmp_004311"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:4575..4577,aa:Arg,seq:tct)
BASE COUNT         1236 a         1058 c         1242 g         1121 t
ORIGIN      
        1 aacataatgg caggtttttt aatattcctg tcttctgctg cttatgctga tatcaatctg
       61 tatggtcctg gtggcccgca tacagccttg cttgatgcag ccaaacttta tgccgaaaaa
      121 acaggtatta tagtgaacgt tcattacggc ccacagaaca aatggaatga agatgccaaa
      181 aaaaatgcag atatcttgtt tggcgcatca gaacaatctg ctctggctat cattcgggac
      241 cataaagaca gcttcagtga aaaagatatt cagcctcttt atctgcgaaa aagtatttta
      301 ctggtaaaga aaggtaatcc taaaaatatc cggagtattg acgacctgac cagacctggg
      361 attggcgtaa ttgttaatga tggtggtggt accagtaata catcaggcac tggcgtctgg
      421 gaagatattg ccggacgtaa agggaatata gaaactgtcg ccgcaatccg aaaaaatatt
      481 attttatatg cgcccaatag cggaactgca cgtaaggctc ttgagaatca gcctggagca
      541 gatgtctgga taacctgggc tgactgggca gccagtaatc cagaaattgg tgatgtcgtg
      601 gaaatagcgc cagactacgt gatatggcgt gatatgaaca ttacagtacg tcaggatgca
      661 aatgatgaaa cccgtcgatt tgcagaatgg ctacaaaccg atgaagcggc gcctgcattc
      721 aaaaaatatg gctggaccag gaaaggcact tgacatcctc gtccttcagg acgtggattc
      781 tttttccgga tgccgcgcca gcggcatgta ggggcagctc acaaaacgga aaaaattgta
      841 cgctaagcct cgccaggtga actgaattca ttccgatatg ggaattccca tatcgggcga
      901 aaacggtttg ctgtaacggc agagttaagt aggattgctg cgggtgcttg aggctatctg
      961 cctcgggcat gaacaccaac ggcagataga taaaagcccc acccgactat aaatcgaagt
     1021 gaggccccta tatgctcgtc acatatagat tgcctcttac ggaccgaaag gtcaaggaga
     1081 agcaggctat gaagcagcaa aaggcgatgt taatcgccct gatcgtcatc tgtttaaccg
     1141 tcatagtgac ggcactggta acgaggaaag acctctgcga ggtacgaatc cgaaccggcc
     1201 agacggaggt cgctgtcttc acagcttacg aacctgagga gtaagagacc aggcggggga
     1261 gaaatccctc gccacctctg atgtgtcagg catcctcaac gcacccgcac ttaacccgct
     1321 tcggcgggtt tttgttttta ttttcaacgc gtttgaagtt ccggacggcg ccggaataga
     1381 atcaaaaata cttaagtagc gcgcagggag aagagggatg gaccccgaac aggggagtgc
     1441 tatttatctg gaaggattct gttgatgaga atcgaagaat tacgtgaaat ttttagtgaa
     1501 gatggcctct atactgtgcg cgttgagaag ggcgctattg tcagccactg ccgtattaaa
     1561 tgtttacagt ctcaacaaag gaagagtgga gctgcgttaa ttcattttgt ggatgggctt
     1621 gtgacggatg gttttatttt gcgtgcaaat gaatttgtca catcgttgcc gtctctgaaa
     1681 gaagctggga ttaaggctgg tttttctgct tttgaagatt agtgaattca tctacaattc
     1741 agcgcagggc tgaacccctg ttgagtaaca ctgtgccacc ggagaaagcc gatggcgcaa
     1801 aattccagac tacacaattc tgataattca gccgtctttg ccagcaggca cgggcggcgt
     1861 tctcatgcat tcaaatctga ctggttccgg cacgccccat gcactgaaga acaggccgaa
     1921 tggctgattc agaactaccg cagacgtggg tatgagttta ggaaagccct cagcctcgat
     1981 tatcgtcact ggataatcta cgtcaggctg ccgtactccg agcgcccacc gcgtccgtcc
     2041 cgcacattcc agcaacgcat ctggaggtaa cgtgcgggta ttacttcgac ctgttccggt
     2101 accggaactt gggttggtgg tccttaaacc aggccgtgaa tccatgcagg tattccataa
     2161 ccctcgagtg cttgtggagc cggaaccgaa aagcatgcgc ggtctgccgt ccggcatcgt
     2221 ccctgccgtt cgccagccgc tggcggagga taaatcatta ctgccgtttt tcagcaatga
     2281 gcgtgtgatt cgtgctgttg gtggcgctgg tgcactgtct gactggctgt tgcgtcatgt
     2341 caaatcctgc cagtggcctc atggtgacta tcatcacagt gaaaccgtta ttcaccgtta
     2401 tggtaccggc gcaatggtgt tgtgctggca ctgcgacaac cagctgcgtg accagacatc
     2461 cgaatcactc gagcaacttg ctcatcaaaa cctgtcagca tggatgattg acgtcatcgg
     2521 tcacgcaata agcggtacgc aggagcgtga attatctctg gctgaattat cctggtgggc
     2581 ggtccgcaat caggtggcgg acgcgctacc ggaagcggta ttacgtcgtt cgctggggtt
     2641 gcgtgcggaa aaaatccgct caatgtaccg tgaaagcgac atcgtaccgg gagagcagac
     2701 cgccaccatc atactgaagc agcgcacaaa aaatcttgcg ccgctgcctc acgcccacca
     2761 gcaaaacccg ccacaggaaa agacggtggt cagcattgcc gttgatccgg agtcaccggc
     2821 tcagtatctc cagcgccaga aaccacaacg ggaagagatg cctgtataca cgcgctgggt
     2881 aaaaacgcag aaatgcatga cgtgcggtaa tcaggcagat gatccgcatc acatcattgg
     2941 tcatggactg ggagggatgg gaacaaaggc tgatgatttg tttgttattc cgctgtgccg
     3001 taaatgccat agcgaactac acgccggggt aaaagatttt gaagaaaaac acggcagcca
     3061 gctgttgttg ctgattcgtt ttttaatgca cgcgagaaat tcgggtgtcc tgaagtggaa
     3121 agcatgaatg actgaacgga tagaatttgt tttgccttac ccgccgacgg tgaataccta
     3181 ctggcgacgt catggcaata cgtatttcat ctcggaagcc ggaaagcgtt atcgccgtga
     3241 tgtggcgcta attgttcgcc agcagcggct gaaattaaac ctgtccggaa ggctggtgat
     3301 aaagattatt gcagagccac cggataagcg ccgtcgtgac ctggacaata tcctgaaagc
     3361 accactggat gtgctgacgc atgccggact tctcatagac gacgagcagt ttgatgaaat
     3421 caatattgtg cgcggtcagc tcgttcctgg tgggcggctg gggataaaaa tcacagaact
     3481 ggagtgcgca tgaataacca ctatttacag tttgtgcgtg agcagctcat tatcgccacc
     3541 gctgatttga gtggggcaac aaaaggtcag cttgaagcct ggcaggagaa tgccatgttc
     3601 gatacagggc gttacaggcg aaaaaaaatc cggtaccgcg atgaagtgac tggaaaaatg
     3661 ataacgcggg ataatccacc aatcccggga aagcaatcgc tggcgaaggt gacgtcaatt
     3721 cctctggtca gtccggttga gttttcgaca tcatcgtggc ggcgggctgt tctgtctctt
     3781 gaagaacatc ataaagcctg gttgttgtgg tgttacagcg ggagtatttg ttgggaatat
     3841 cagatcgcga taacacagtg ggcgtggaat gaatttaata ctcaatccgg taccagaaaa
     3901 attgctggga aaacgcagga acgcctgaaa aaattaatct ggctggcggc gcaggcagta
     3961 aaagcagaac tttttggtgg ggaaggttat gaatatcagg agctggcatt actggcggga
     4021 gtgacaacta aaaactggtc caaaacattt actcgtcact gggttgcaat gaaacacatt
     4081 tttcaccgac tggatagtga ggctttatta tttgtaatga gaacacgttc aaaacaaaag
     4141 gcggcatttt caaagcaaag tgttgcaaaa gtagattgaa aggcatatat ttcatgcaaa
     4201 tctgatattt tgccgatttt gtacgtgatg gcaaaagcaa acaaaacccg cccacaagcg
     4261 ggttttgttg tgccacttat ctcggataga catggtgaat gcgctggtgg aggagataag
     4321 ggtgattttt gtatgcttgc aacattgatt tcgtaacgtt attatcctgc gcccggccct
     4381 ttagctcagt ggtgagagcg agcgactcat aatcgccagg tcgctggttc aaatccagca
     4441 agggccacca accgccacta gccatcagga aagagcgtca accctttaag ttgagtgtgc
     4501 gaggttcgag tccccggtgg tggtccatta tcggtattct gcgttgttag ctcagcagga
     4561 cagagcaatt gccttctaag caatcggtca ctggttcgac tccagtacaa cgcgctacac
     4621 ttattttccc ggctcgcttt tgcgggcctt ttttgta
//
LOCUS       DAZKHF020000296         4654 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2201S31241
            SAMN43267800-rid25106413.denovo.298, whole genome shotgun sequence.
ACCESSION   DAZKHF020000296 DAZKHF020000000
VERSION     DAZKHF020000296.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267800
            Sequence Read Archive: SRR30307263
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4654)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4654)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 4654)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350581.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350581.2
            Genome Coverage       :: 151x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:21:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,652
            CDSs (total)                      :: 5,557
            Genes (coding)                    :: 5,320
            CDSs (with protein)               :: 5,320
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 237
            CDSs (without protein)            :: 237
            Pseudo Genes (ambiguous residues) :: 0 of 237
            Pseudo Genes (frameshifted)       :: 102 of 237
            Pseudo Genes (incomplete)         :: 130 of 237
            Pseudo Genes (internal stop)      :: 52 of 237
            Pseudo Genes (multiple problems)  :: 41 of 237
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4654
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267800-rid25106413.denovo.298"
                     /serotype="O 26:H11"
                     /isolate="2201S31241"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-01"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            437..1000
                     /locus_tag="ACHOSC_RS21560"
                     /old_locus_tag="pgaptmp_004312"
     CDS             437..1000
                     /locus_tag="ACHOSC_RS21560"
                     /old_locus_tag="pgaptmp_004312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inovirus-type Gp2 protein"
                     /protein_id="HGU4550116.1"
                     /translation="MYNANPNYEMDFMILKDVNEHMEGLFQRFSKLLPFRIDFAYRKD
                     TPSFGHSCRHSMCIEMHRLLSETQTMLAGYYWVMEYTSNKGLHIHFIGYLDGQRHNKS
                     YRISRQLGDIWRRITEGEGYFHLCRAKDKYPVRIDHVIHYSDKSAVDDLRYALSYLAK
                     QDQKEHGIILRRSRLPEKSNRGRPRHN"
     gene            1155..3515
                     /locus_tag="ACHOSC_RS21565"
                     /old_locus_tag="pgaptmp_004313"
     CDS             1155..3515
                     /locus_tag="ACHOSC_RS21565"
                     /old_locus_tag="pgaptmp_004313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
                     /protein_id="HGU4550117.1"
                     /translation="MVHKSDSDELAALRAENARLVSLLEAHGIEWRRKPQTPVQRVSV
                     LSTDEKVALFRRLFRGRDDVWALRWESKTSGKSGYSPACANEWQARICGKPRIKCGDC
                     AHRQLIPVSDLVIYHHLAGTHTVGMYPLLEDDSCYFLAVDFDEAEWQKDASAFMRSCD
                     ELGVPAALEISRSRQGAHVWIFFASRVSAREARRLGTAIISYTCSRTRQLRLGSYDRL
                     FPNQDTMPKGGFGNLIALPLQKRPRELGGSVFVDMNLQPYPDQWAFLVSVTPMNVQDI
                     EPTILRATGSIHPLDVNFINEEDLGTPWEEKKSSGNRLNISIAEPLKITLANQIYFEK
                     AQLPQVLINRLIRLAAFPNPEFYKAQAMRMSVWNKPRVIGCAENYPQHIALPRGCLDS
                     VLSFLRDNNIAAELIDKRFAGTECNAVFMGNLRAEQEEAVSALLRYDTGVLCAPTAFG
                     KTVTAAAVIAKRKVNTLILVHRTELLKQWQERLAVFLQAGDSIGIIGGGKHKPCGNID
                     IAVVQSISRQGEVEPLVRNYGQIIVDECHHIGAVSFSAILKETNARYLLGLTATPIRR
                     DGLHPIIFMYCGAIRHTAVRPKESPHNLEVLIRSRFTSGHLPSDARIQDIFREIALDH
                     DRTVAIAEEAMKAFGQGRKVLVLTERTDHLDEIASVMNSLKLSPFILHGRLSKKKRAM
                     LISGLNALPPDSPRILLSTGRLIGEGFDHPPLDTLILAMPVSWKGTLQQYAGRLHREH
                     TGKSDVRIIDFVDTAYPVLLRMWDKRQRGYKAMGYRIIADGDESVI"
     gene            complement(3677..3853)
                     /locus_tag="ACHOSC_RS21570"
                     /old_locus_tag="pgaptmp_004314"
     CDS             complement(3677..3853)
                     /locus_tag="ACHOSC_RS21570"
                     /old_locus_tag="pgaptmp_004314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000136079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4550118.1"
                     /translation="MTEILRIAQTYEPWLDFWELVVQNVSTIASHTATNRPLPEAIMA
                     NYQLDEQLLEGCRP"
     gene            complement(3991..4233)
                     /locus_tag="ACHOSC_RS21575"
                     /old_locus_tag="pgaptmp_004315"
                     /pseudo
     CDS             complement(3991..4233)
                     /locus_tag="ACHOSC_RS21575"
                     /old_locus_tag="pgaptmp_004315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004389801.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
     gene            complement(4272..>4654)
                     /locus_tag="ACHOSC_RS21580"
                     /old_locus_tag="pgaptmp_004316"
     CDS             complement(4272..>4654)
                     /locus_tag="ACHOSC_RS21580"
                     /old_locus_tag="pgaptmp_004316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000998048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HGU4550119.1"
                     /translation="LKSLESWLREKMKTLSRHSELAKAFAYALNQWPALTYYANDGWV
                     EIDNNIAENALRAVSLGRKNFLFFGSDHGGERGALLYSLIGTCKLNDVDPESYLRHVL
                     GVIADWPVNRVSELLPWRIALPAE"
BASE COUNT         1167 a          989 c         1185 g         1313 t
ORIGIN      
        1 ttatggtgtg tggggtatgg tctgtatacc atcattttta tggtgaagtt aaatatctgt
       61 agaggcctca agctgggtcg ctgtttttac ttaatggtac agaagtatta atagcggtgt
      121 tgcctgtggc tacccgatac tggcttttgt gaatgtcaga ttacttcctg taagtgattg
      181 aatggtactt ccttaaactc aattctatga ggtgtgtttt taccattgta attcgtactg
      241 gtcctcagtt taagtgagtc ttactgtact ctgaaatatt acctctttgg agtaatgagg
      301 taatacgaaa cagttttatc aggttgtctg tgctgctggt tacctcgttt attaatgccg
      361 cggtacggcg tttctgtact ctcagtattt atgtaattcc accttcctgt aacttttata
      421 aagtaaggac tataaaatgt ataatgctaa tcccaattac gaaatggact tcatgattct
      481 gaaagacgtg aatgaacaca tggaaggtct gtttcaacgt ttttctaaac tgttaccttt
      541 cagaattgat tttgcctaca ggaaagacac gcccagtttt ggtcattcct gtaggcacag
      601 tatgtgtatt gaaatgcacc gcttacttag tgaaacccaa acgatgctgg cgggatatta
      661 ctgggtaatg gaatatacgt caaacaaggg acttcatatt cattttatcg gctacctgga
      721 tgggcaacgt cacaacaaat cttatcggat atccaggcaa ctgggagata tctggaggcg
      781 gatcacggaa ggggagggtt attttcacct gtgcagggct aaagacaaat acccggtgcg
      841 tatcgatcac gtaattcact atagtgataa atctgcggta gacgacttac gttacgccct
      901 tagctatctg gccaaacagg atcagaaaga acacggtatt attctgaggc gtagcaggct
      961 tcccgaaaaa agtaaccggg gccgaccacg acataactga attacagctg tattaggtat
     1021 tagaattaag tttctgtaag ttgttgggag gtgcttgagt taataggcag aagagggcgg
     1081 ggcgctttgt catcagtgta ggggacccga atttctgccg tggtgcctca gtggatcgga
     1141 tacaggtaac gaatatggtt cataaatctg acagtgatga attagctgca ttgagggcag
     1201 aaaatgcccg tctggtctca ttacttgaag ctcatgggat tgaatggcga cgtaaaccgc
     1261 agactcctgt gcagcgcgtt tctgtattat ccaccgacga gaaggttgca ttatttcgtc
     1321 ggttgtttcg tgggcgtgat gatgtatggg cacttagatg ggaaagtaaa accagcggca
     1381 aatcagggta ctctcctgcc tgcgctaacg aatggcaggc gagaatatgc ggtaaacccc
     1441 ggataaaatg tggggactgc gctcaccgtc agttgattcc tgtatccgat ctcgtcatct
     1501 accaccatct ggccggtacc catactgtcg ggatgtatcc gttgctggaa gatgattcct
     1561 gttattttct ggcagttgat tttgatgaag ctgaatggca gaaggatgca tccgcattca
     1621 tgcgatcctg tgatgagctg ggtgtacctg ctgcgctgga aatatcccgt tcacgtcagg
     1681 gggcgcatgt ctggatattt tttgcctcac gagtttcggc ccgcgaagct cgccgtctgg
     1741 ggactgctat tattagctat acgtgtagtc ggacccgaca gctgcgattg gggtcttatg
     1801 accgattatt tcctaatcag gatactatgc caaaaggggg atttggtaat ctcatagcgt
     1861 tacctctgca aaaaagaccg cgtgaattgg ggggaagcgt ttttgttgat atgaatctcc
     1921 agccttatcc tgatcagtgg gcttttcttg tatcggtgac cccgatgaat gtgcaggata
     1981 ttgaaccgac gatattacgg gctacaggga gtatccatcc tctggatgtg aattttatca
     2041 acgaagaaga cctgggtacg ccgtgggaag agaaaaaatc atcaggaaac agactgaata
     2101 tttctattgc agaaccgctg aaaatcacgc tggcaaacca gatctatttc gaaaaagcgc
     2161 aattacctca ggtgctgatt aaccgactta ttcggctggc agcatttccg aaccctgagt
     2221 tttataaggc tcaggcaatg cgtatgtcag tctggaataa gccccgtgtt ataggttgtg
     2281 cggagaatta cccgcaacac attgcgttgc cccggggatg tctggacagc gtattatctt
     2341 tccttaggga caacaatatt gctgcagaat taatcgataa acgatttgcc gggacggaat
     2401 gtaatgccgt ttttatggga aacctcagag cggagcagga agaggccgtt tcggcattac
     2461 tccgttatga cactggtgtg ctttgtgcgc caacggcttt tggtaagaca gttaccgcag
     2521 cggcagtgat tgccaagcgg aaagtgaata cactgatact ggtacaccgg actgaattgc
     2581 tgaagcagtg gcaggagcgt ctcgcggtgt ttctgcaggc cggtgacagt attggtatta
     2641 tcgggggagg taaacataaa ccctgtggca atattgatat tgcggtggtg cagtccatat
     2701 ccagacaagg agaagttgaa cctctggtca ggaattatgg gcaaatcatt gtggatgagt
     2761 gccatcatat tggcgcggtt tcattttctg cgattctgaa ggaaacgaat gccagatatc
     2821 tgcttggcct gacggcaaca ccaatccgac gggatggtct gcatcccatt atttttatgt
     2881 actgtggtgc cattcgccat acagcggtcc gcccgaagga aagcccacat aatctggagg
     2941 tactgatccg ttcccgtttt acatctggtc atttaccatc ggatgcgaga atccaggata
     3001 ttttcagaga aattgctctg gatcatgaca gaacggtggc gatagctgaa gaagccatga
     3061 aagctttcgg gcaggggcga aaagttctgg tactgactga acgtacagat catctggatg
     3121 agatagcatc agtgatgaat tcactgaaat tgtctccctt tattctccat ggtcgactat
     3181 cgaaaaaaaa gcgtgcgatg ctgatatccg ggctgaatgc tcttcctccc gattctcctc
     3241 gaattttgtt gtcaacaggc agacttattg gtgagggatt tgaccaccct ccgctggata
     3301 cgctgattct tgccatgcct gtgtcatgga aagggacatt acagcagtat gcagggcgtc
     3361 ttcacagaga gcataccggt aaaagcgatg tcaggatcat tgattttgtg gataccgcgt
     3421 atcctgtgct gctcagaatg tgggataaac gtcagcgggg ttataaagcg atggggtaca
     3481 ggattatagc tgacggcgat gaatcagtga tttaatttta aggagtactt tacccttttt
     3541 aaataatttg agctccccgg gttgttcggg caacaaagag tcccagtata caggcttcag
     3601 gaatatgttg tagtatcagg cttttcatcg ctttaaaatg actaccggca gtgagaacgt
     3661 catcaaaaat aacaatctag ggcctgcacc cttccagtaa ttgttcatcc agttggtagt
     3721 ttgccataat cgcttctggt aagggccggt ttgttgcggt atgagaggct attgttgata
     3781 cgttttgcac aaccagttcc cagaaatcca gccatggttc gtaggtttgc gcaattcgca
     3841 gaatttctgt catacggtca tcgtaagcgg catctgtacg gcatttggat ggtggtacgg
     3901 gaacaaatgt gtattcctca agattaccga tatttgatat gagcatctga gcaacatact
     3961 caatagcagc ctttttatag tggtaatcag gctacgaaag tacagcgttt tataaattgt
     4021 ctcaggtgat attcgcagcg ttttttgacg tggttttgtt cgccttaacc atcctgatat
     4081 ttgctctgga gaccatttca tctccagctt ttccagaaca agctttcgca atggtaaatt
     4141 ttgatccagt aagcacggtt ttggcctttt cgccattctg ttggctcggt tattagcatc
     4201 aacagctttg taatagcgtc tgccccgatt acgcgtaagc gtacagcgag ggccgtattg
     4261 acggggatgt gttattcagc tggcagtgct atgcgccacg gaagcagttc gctgacccgg
     4321 ttgaccggcc agtctgctat gacgccaagc acatggcgaa ggtagctttc tggatccacg
     4381 tcattcagtt tgcacgtccc gatcaggctg tacagtagcg ctccccgctc accaccatga
     4441 tcagagccga agaacaggaa gtttttacga cccagactga ccgcccgcag ggcattttca
     4501 gcgatgttgt tgtcgatttc cacccagcca tcgttcgcat agtacgtcag tgccggccac
     4561 tggttaagtg cgtacgcgaa cgccttcgcc aactctgagt gtcgcgacag ggtcttcatc
     4621 ttttcacgca accagctttc cagggatttc aaca
//
LOCUS       DAZKHF020000297         4653 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2201S31241
            SAMN43267800-rid25106413.denovo.299, whole genome shotgun sequence.
ACCESSION   DAZKHF020000297 DAZKHF020000000
VERSION     DAZKHF020000297.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267800
            Sequence Read Archive: SRR30307263
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4653)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4653)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 4653)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350581.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350581.2
            Genome Coverage       :: 151x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:21:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,652
            CDSs (total)                      :: 5,557
            Genes (coding)                    :: 5,320
            CDSs (with protein)               :: 5,320
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 237
            CDSs (without protein)            :: 237
            Pseudo Genes (ambiguous residues) :: 0 of 237
            Pseudo Genes (frameshifted)       :: 102 of 237
            Pseudo Genes (incomplete)         :: 130 of 237
            Pseudo Genes (internal stop)      :: 52 of 237
            Pseudo Genes (multiple problems)  :: 41 of 237
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4653
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267800-rid25106413.denovo.299"
                     /serotype="O 26:H11"
                     /isolate="2201S31241"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-01"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            419..1435
                     /locus_tag="ACHOSC_RS21585"
                     /old_locus_tag="pgaptmp_004317"
     CDS             419..1435
                     /locus_tag="ACHOSC_RS21585"
                     /old_locus_tag="pgaptmp_004317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000100143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4550120.1"
                     /translation="MSSVRTDKFIRYGFAVVAIVAILITTITTPNPTPYQYIVYRIVL
                     VLAAGCIGAVLPGFLEVKFNGWLRASGAVAIFAIVFFISPDLLQQNPESASKPVPPTV
                     IDAYAVNSSNRDRYLELFRTGQFGQRDKLRIGCVEWSESSCVAAGNFLRLLSEAGWEI
                     DSDAVFRMQPSVPVEGVSIVSRGDDLVGLPKVPPHMDRWSRSDISYEILTAAFKLMGV
                     PVYFSSDPTLQPGTTGVYFGPKPKLDAPVMQKDENVRRELLRYVGLGMTIQRACSQQP
                     VELCGGEIFSWETSVSSFLRQQRFNSDAVNRWLALPQVNAASATADMKIKLDLLMSFV
                     LGIE"
     gene            2079..4439
                     /locus_tag="ACHOSC_RS21590"
                     /old_locus_tag="pgaptmp_004318"
     CDS             2079..4439
                     /locus_tag="ACHOSC_RS21590"
                     /old_locus_tag="pgaptmp_004318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000839829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I restriction enzyme HsdR N-terminal
                     domain-containing protein"
                     /protein_id="HGU4550121.1"
                     /translation="MKSMQSKTGSPLVRTEAELESRLTSALNIAFPNIPREDLIEQRH
                     FTVRLGHGTYKIDSAAHWKKYGRADVLIFHRERPLAVIELKREDLTLTHDDYEQAQSY
                     ANQLTPRPPLVVVTNGKDTRVYDSSNGQQWSGGQDASAAVNKMLANSAKLAAADMRWA
                     IEALMGRETNAWVPAVREETARLLIDITDQPGHSEHPFANNLLFPRKITSLVIESAVM
                     GTAFTIIEGDAQSGKSSCLREISLKTESSDLLAVLMLRGSGPGLFQALANLFAAEFEW
                     NLTSNDARNWLRRMSNCTEGPSLMLAIDDVEPGSQMATDLEELAGIRFGNRLVVVLTT
                     YHANALLKNPNGRTPSAIGSRSKVFKTSPMSLDEFKLAQQILSDQRIVFQQGAEYADD
                     YRSPWVLRTIYDDIVRNHQYQKTDLIAYLPPSPGMELIDAAQKSYESQYDLLRYYRVL
                     ARCALADTNSHSVELMFAKANGFVVRYDALSDEARGVVNELKHMGAVRIFRLSGWEDV
                     VVPTVPAAYLLELSDAVCDELVLRAEQDPQDAGAWLGERLDATYLGDMIGAQAIRRMA
                     AKERYFSFGIIQGLLSIEPYKEPIKNGLFTLAMPDNQQVNLKIEDGLAWISKHGDDAK
                     SVLVNLEDQIPKVISKSTSWMILGQLAKLPSAEVGDDDQRIDAYILLSIGRCPFPLIR
                     TNIEGLPYFEHNFGDQGDVLCLEKASIEVATQAMADLFSAPWLYADQWVDTAIATGSI
                     HLLHRLFAALNTVILRRIPVQSDWANEALNQRVSPALKEAIRSLSS"
BASE COUNT         1225 a          998 c         1127 g         1303 t
ORIGIN      
        1 acttacttgg ctttagtccc ggagtgttgc caggagtatc tttattatct cgtcggcgtt
       61 gatctggttt tcctgtcgat ttagcaattg gttttggacc aggtttaaga cccatgatag
      121 ttatcctttt gagatgtaag agggaagccc tcaacattgg tatattggat actgagaaat
      181 ttttcaatgg gagtgataaa cggccgaatg attaactcga tattgtgatc aacgtctgtt
      241 ttttgcacac tgtagactga ctcttggagc agaaggttgc tctgagcgag gaatggacag
      301 ttacactcat caccaacgcc aatagcctca ttacgcgtat aaagctgtat taatcattgg
      361 ggagcagttt aatgcgggat tttcgactta tggacttgat tcaggtgtca aaattaaaat
      421 gagcagtgta aggacagaca agttcattag gtatggtttt gcggttgtgg cgatagttgc
      481 catactcatt actactatca caacccccaa tcccacacct tatcaatata tagtttaccg
      541 aatagttctg gttctggctg ccggatgtat tggtgcagtt ctgcctggtt tccttgaagt
      601 aaaatttaat gggtggttaa gagcatctgg cgcagtggcg atcttcgcta tagtgttttt
      661 cataagccca gatttgcttc aacaaaatcc tgaatctgca agtaaacctg taccgcctac
      721 tgtgattgat gcatatgccg ttaatagttc caatcgcgac agatacctcg aattatttcg
      781 aacaggacag ttcggtcagc gggacaagct gcgcatcggt tgtgttgagt ggagtgaatc
      841 atcgtgcgtg gctgctggta attttttgcg attgttaagc gaagctggct gggaaatcga
      901 ttctgacgct gtattcagaa tgcaaccatc cgtgccagtt gagggggtat cgattgtaag
      961 ccgtggcgat gatctggtcg gtttgccaaa agtaccaccc cacatggacc gttggagtcg
     1021 atcagacatt agttatgaaa ttttgacagc ggcatttaaa ttgatgggag ttccggttta
     1081 tttttccagt gacccgacat tacaaccagg tactaccgga gtgtactttg gaccgaaacc
     1141 gaaactggat gcaccagtca tgcaaaaaga tgagaatgtg cgacgtgagc ttctccgcta
     1201 cgtcggtttg ggaatgacta ttcagcgagc ctgctctcag cagcccgtcg aactttgtgg
     1261 gggtgaaatc ttttcctggg aaacgtcggt atcttcgttc ctaaggcagc agagattcaa
     1321 ctccgatgcc gtaaacagat ggcttgcctt gccgcaggtt aatgcggctt cggcaactgc
     1381 agacatgaaa ataaagctag atttgttaat gtcatttgtt ctgggcatcg agtaactata
     1441 cctcttgcga ggatgattga gcgtctaatg gaccagctta gccgttagcg ctatttgttt
     1501 acgtaagcct gtggaatcca actagtgctt ctacgatgcg ttgtttggtg aaaaaaaagt
     1561 gttgaagcac tggttttaga caactaaagc cacgatttgc tcatgcgtcg aaaatgtagt
     1621 cgtctactgt gtagcacgga ggcgatattc gtacgcttac acttgccgat aggccactgg
     1681 ggagtgttct tggcactaag ctgccaggcc cgaaggtctg ctatgagcga ggggctgaca
     1741 ttcctattgg cattcggtgt gagttatcgg gggcggtcat gcggcatact tccgatacag
     1801 gcgtttccag ctctgcctgt ttcagggtta tattaatctg ctttagaatt aatcgtggct
     1861 ttagcatctg cacatcgtgc tggagaaacc tgagtgaact aaatcatatt ccctttcttc
     1921 agctaatcaa ttgatagtta tgcggaaaaa gttactttgc ttgtcaataa tccatttcac
     1981 atccgttaat ccaatgctat gcgtcttcgc attaatctgg aaaggtagta agtgaaataa
     2041 aaacttagcc aatgactaat atctgaggat actttgtcat gaaaagtatg caatcaaaaa
     2101 cgggctctcc actcgttcga actgaagccg agctagaaag ccggcttact tcagcactaa
     2161 acatagcatt tcctaacatt cctcgcgaag atcttatcga acagcgccat tttaccgttc
     2221 ggctaggcca tggaacttac aaaatagata gcgccgcaca ctggaaaaaa tacgggcgag
     2281 cggatgtttt gatctttcat cgggagagac cacttgcggt tattgaactg aaacgtgaag
     2341 acctgacact gacccatgat gattatgaac aggctcaaag ctacgctaac cagttaacac
     2401 cacgtccgcc tctcgttgta gttacgaacg gtaaagatac gcgcgtttat gactcaagta
     2461 atggacaaca gtggtcgggt ggacaggatg cctcggcagc ggtaaacaag atgctcgcca
     2521 attctgcaaa gttggcggcg gctgacatgc gctgggctat tgaagccttg atggggcgag
     2581 aaactaatgc ttgggtgcca gctgtgcgag aagaaacagc ccggctgctg attgatataa
     2641 ctgatcagcc tgggcattca gaacatccat ttgccaacaa cctacttttt cctcgcaaaa
     2701 tcacgtctct tgtaattgaa tctgctgtga tgggtacagc tttcactatc attgagggtg
     2761 atgcacaaag cggaaaaagc agttgcctcc gtgaaatttc gctaaaaacg gaaagtagcg
     2821 atcttttggc tgtcctgatg ttacgaggtt ccggaccagg actatttcag gctctggcca
     2881 atctttttgc agctgaattt gaatggaatt taacctcaaa tgatgcacgt aactggttaa
     2941 gacgaatgtc taactgcact gaaggtccat cgcttatgct tgctatcgac gacgttgaac
     3001 caggctccca aatggctact gatcttgagg aacttgcggg tatacgtttt ggcaacaggc
     3061 tggtggttgt tctgacgacc tatcatgcca acgcgttact gaaaaatcca aacggacgaa
     3121 ctccgtctgc aattggtagc cggtcgaagg tatttaaaac atcgcctatg tccctagatg
     3181 aatttaagct ggcgcaacag attctctcgg accagcggat agtttttcaa cagggagcag
     3241 aatacgccga tgactaccgc tcgccttggg ttcttcgcac tatttatgat gacattgtgc
     3301 gcaatcatca ataccagaaa acggatctta tcgcctatct tcctccatca cccggtatgg
     3361 aacttataga tgctgcgcaa aagagctatg agagtcagta tgatcttctt cgttattatc
     3421 gggttcttgc acgctgcgca ctagctgaca caaactccca ctcagtagaa ttaatgtttg
     3481 ctaaagcgaa tggatttgtc gtgcggtatg acgcactttc tgacgaagct cgtggtgtag
     3541 tgaatgagtt aaaacatatg ggggcagttc gtatttttcg cctttcagga tgggaagatg
     3601 ttgtggtgcc gacagttccc gcagcttacc ttctggaact gtcggatgct gtttgtgatg
     3661 aactggtttt acgggcagaa caagaccctc aagacgccgg ggcttggctt ggcgaacggc
     3721 tagacgccac ttatcttgga gatatgatcg gtgctcaagc catacgccgt atggcagcta
     3781 aagagagata tttcagcttt ggaattattc agggccttct cagcattgag ccatataaag
     3841 aacctataaa aaacggattg tttactttag ccatgccaga caaccaacaa gtcaatctaa
     3901 agattgagga tggattagcg tggatttcta aacacgggga tgatgcaaag agtgttctgg
     3961 tgaatttaga ggatcaaatc ccaaaggtca tttctaaatc aaccagttgg atgatactcg
     4021 ggcaactagc gaaactgccg tcagcagagg tgggtgacga tgatcagcgt atagacgcct
     4081 atattttact gagcattggg cgctgcccat ttcctctaat tcgcacgaat atagaaggat
     4141 tgccttactt tgagcataat tttggcgatc agggtgatgt tctttgccta gaaaaagcgt
     4201 ctattgaagt tgctactcag gcgatggctg atttattttc agccccatgg ctctatgcgg
     4261 accagtgggt ggatacagct atcgcgaccg gttctataca tttactgcat cgtttgtttg
     4321 ctgctttaaa cacagtgata ttgcgtagaa tacccgtgca atccgactgg gctaatgaag
     4381 cacttaatca acgtgtctca ccagccctta aggaagccat acgatcatta tcttcgtgac
     4441 taagcaatgg gattttaata cataacgagc atctgtccac gcaagttaac aattgccttg
     4501 gctcgatatt ttggtttcta gtgcatatat gctgatcttc tcttcgttga tctgatctta
     4561 cccaccaata gtggacacag gactaagtga gtaaactctc aaccagaggt gactcatgac
     4621 aaaaccagta tcaatcagca agaagccccg tga
//
LOCUS       DAZKHF020000298         4532 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2201S31241
            SAMN43267800-rid25106413.denovo.300, whole genome shotgun sequence.
ACCESSION   DAZKHF020000298 DAZKHF020000000
VERSION     DAZKHF020000298.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267800
            Sequence Read Archive: SRR30307263
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4532)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4532)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 4532)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350581.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350581.2
            Genome Coverage       :: 151x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:21:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,652
            CDSs (total)                      :: 5,557
            Genes (coding)                    :: 5,320
            CDSs (with protein)               :: 5,320
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 237
            CDSs (without protein)            :: 237
            Pseudo Genes (ambiguous residues) :: 0 of 237
            Pseudo Genes (frameshifted)       :: 102 of 237
            Pseudo Genes (incomplete)         :: 130 of 237
            Pseudo Genes (internal stop)      :: 52 of 237
            Pseudo Genes (multiple problems)  :: 41 of 237
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4532
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267800-rid25106413.denovo.300"
                     /serotype="O 26:H11"
                     /isolate="2201S31241"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-01"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            complement(<1..291)
                     /gene="chbB"
                     /locus_tag="ACHOSC_RS21595"
                     /old_locus_tag="pgaptmp_004319"
     CDS             complement(<1..291)
                     /gene="chbB"
                     /locus_tag="ACHOSC_RS21595"
                     /old_locus_tag="pgaptmp_004319"
                     /EC_number="2.7.1.196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS N,N'-diacetylchitobiose transporter subunit
                     IIB"
                     /protein_id="HGU4550122.1"
                     /translation="MEKKHIYLFCSAGMSTSLLVSKMRAQAEKYEVPVIIEAFPETLA
                     GEKGQNADVVLLGPQIAYMLPEIQRLLPNKPVEVIDSLLYGKVDGLGVLKAAV"
     gene            complement(590..928)
                     /gene="osmE"
                     /locus_tag="ACHOSC_RS21600"
                     /old_locus_tag="pgaptmp_004320"
     CDS             complement(590..928)
                     /gene="osmE"
                     /locus_tag="ACHOSC_RS21600"
                     /old_locus_tag="pgaptmp_004320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="osmotically-inducible lipoprotein OsmE"
                     /protein_id="HGU4550123.1"
                     /translation="MNKNMAGILSAAAVLTMLAGCTAYDRTKDQFVQPVVKDVKKGMS
                     RAQVAQIAGKPSSEVSMIHARGTCQTYILGQRDGKAETYFVALDDTGHVINSGYQTCA
                     EYDTDPQAAK"
     gene            1130..1957
                     /gene="nadE"
                     /locus_tag="ACHOSC_RS21605"
                     /old_locus_tag="pgaptmp_004321"
     CDS             1130..1957
                     /gene="nadE"
                     /locus_tag="ACHOSC_RS21605"
                     /old_locus_tag="pgaptmp_004321"
                     /EC_number="6.3.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003952 - NAD+ synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_function: GO:0004359 - glutaminase activity [Evidence
                     IEA];
                     GO_function: GO:0008795 - NAD+ synthase activity [Evidence
                     IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ammonia-dependent NAD(+) synthetase"
                     /protein_id="HGU4550124.1"
                     /translation="MTLQQQIIKALGAKPQINAEEEIRRSVDFLKSYLQTYPFIKSLV
                     LGISGGQDSTLAGKLCQMAINELRQETGNESLQFIAVRLPYGVQADEQDCQDAIAFIQ
                     PDRVLTVNIKGAVLASEQALREAGIELSDFVRGNEKARERMKAQYSIAGMTSGVVVGT
                     DHAAEAITGFFTKYGDGGTDINPLYRLNKRQGKQLLTALGCPEHLYKKAPTADLEDDR
                     PSLPDEVALGVTYDNIDDYLEGKNVPEQVARTIENWYLKTEHKRRPPITVFDDFWKK"
     gene            2187..3074
                     /gene="cho"
                     /locus_tag="ACHOSC_RS21610"
                     /old_locus_tag="pgaptmp_004322"
     CDS             2187..3074
                     /gene="cho"
                     /locus_tag="ACHOSC_RS21610"
                     /old_locus_tag="pgaptmp_004322"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease Cho"
                     /protein_id="HGU4550125.1"
                     /translation="MVRRLTSPRLEFEAAAIYEYPEHLRSFLNDLPTRPGVYLFHGES
                     DTMPLYIGKSVNIRSRVLSHLRTPDEAAMLRQSRRISWICTAGEIGALLLEARLIKEQ
                     QPLFNKRLRRNRQLCALQLNEKRVDVVYAKEVDFSRAPNLFGLFANRRAALQALQSIA
                     DEQKLCYGLLGLEPLSRGRACFRSALKRCAGACCGKESHEEHALRLRQSLERLRVVCW
                     PWQGAVALKEQHPEMTQYHIIQNWLWLGAVNSLEDATTLIRTPAGFDHDGYKILCKPL
                     LSGNYEITELDPANDQRAS"
     gene            complement(3034..3609)
                     /gene="ves"
                     /locus_tag="ACHOSC_RS21615"
                     /old_locus_tag="pgaptmp_004323"
     CDS             complement(3034..3609)
                     /gene="ves"
                     /locus_tag="ACHOSC_RS21615"
                     /old_locus_tag="pgaptmp_004323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310475.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="environmental stress-induced protein Ves"
                     /protein_id="HGU4550126.1"
                     /translation="MEYFDMRKMSVNLWRNAAGETREICTFPPAKRDFYWRASIASIA
                     ANGEFSLFPGMERIVTLLEGGEMFLESADHFNHTLKPLQPFAFAADQVVKAKLTAGQM
                     SMDFNIMTRLDVCKAKVRIAERTFTTFGSRGGVVFVINGAWQLGDKLLTTDQGACWFD
                     GRHTLRLLQPQGKLLFSEINWLAGHSPDQVQ"
     gene            complement(3812..4297)
                     /gene="spy"
                     /locus_tag="ACHOSC_RS21620"
                     /old_locus_tag="pgaptmp_004324"
     CDS             complement(3812..4297)
                     /gene="spy"
                     /locus_tag="ACHOSC_RS21620"
                     /old_locus_tag="pgaptmp_004324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-independent periplasmic protein-refolding
                     chaperone Spy"
                     /protein_id="HGU4550127.1"
                     /translation="MRKLTALFVASTLALGAANLAHAADTTTAAPADAKPIMHHKGKF
                     GPHQDMMFKDLNLTDAQKQQIREIMKGQRDQMKRPPLEERRAMHDIVASDTFDKAKAE
                     AQIAKMEEQRKANMLAHMETQNKIYNILTPEQKKQFNANFEKRLTERPAAKGKMPATA
                     E"
BASE COUNT         1137 a         1095 c         1096 g         1204 t
ORIGIN      
        1 aaccgcagcc ttaagcacgc ctaaaccatc gactttgcca taaagcagcg agtcaattac
       61 ttcaaccggt ttgttgggta acaaacgctg gatttcgggc aacatataag caatctgcgg
      121 ccctaataac acgacatcgg cattctgacc tttttcacca gccagtgttt ccggaaatgc
      181 ttcaataatg accggaactt catatttttc tgcctgtgcg cgcatttttg ataccagtaa
      241 agaggtagac atgcccgcag aacaaaacag ataaatgtgt ttcttttcca taaaactgcc
      301 ctcatcgacg attatctgtc agccagacac tccgcaagcc ttaacctgct tccatgctct
      361 gggtaacttg cgaaaccaac atgacttttt ttgtggctga aaggagtata cggtatagac
      421 ctgagcaata gtatttcttt gaccttataa attgtttctc cttgacctgt ggttatgacc
      481 ctcttcacat ttcgggcaaa taattcgcga agataattaa gaactcaggc caaaaaaaac
      541 cggcgcaatg gccggtttcc gttgttactc aagctttcag acgaattgat tacttcgcag
      601 cctgtggatc agtgtcgtat tcagcacagg tctgataacc ggagttgatg acatgtccgg
      661 tatcatctaa cgcgacaaag taggtttctg ctttaccatc acgttgaccc aggatgtagg
      721 tctggcaagt accgcgagca tggatcatgc tcacttcaga cgaaggttta cccgcaattt
      781 gtgcaacctg cgcccggctc atgccttttt tgacgtcttt caccacaggc tgtacaaact
      841 ggtctttggt acgatcataa gccgtacaac ccgccagcat ggttaatacc gccgctgcac
      901 tcagaattcc tgccatattc ttgttcatat tccgtcctct tgtttatcag cgtgttagat
      961 aagcctggaa tacattgggc gctttttcaa gcccgtgaac gaaacggctc cgctttcaga
     1021 ggattcctgt atgacgtttt aaccaccatt cagcccgctg tcgcttgtcg tttcagtagc
     1081 aacgggttag ctttaaggaa gttttgtctt ttctgtctgg aggggttcaa tgacattgca
     1141 acaacaaata ataaaggcgc tgggcgcaaa accgcagatt aatgctgaag aggaaattcg
     1201 tcgtagtgtc gattttctga aaagctacct gcaaacttat ccgttcatta aatcactggt
     1261 gctcgggatc agcggcggtc aggactccac gcttgccgga aagctgtgcc agatggcgat
     1321 taatgagctg cgccaggaaa ctggcaacga atcactgcaa tttattgccg tacgcctgcc
     1381 ctatggtgtt caggctgacg aacaagattg ccaggatgcc attgccttta ttcaaccgga
     1441 tcgcgtatta accgttaata tcaagggcgc ggtattggct agcgagcagg cattgcggga
     1501 agcaggcatt gaactgagcg attttgtccg tggcaatgaa aaagcgcgtg agcggatgaa
     1561 agcacaatat agcattgcgg gtatgaccag cggtgtcgtg gtgggcaccg atcatgcagc
     1621 agaagccatt accggattct tcactaaata tggtgacggc ggtacggata ttaatccgct
     1681 gtatcgtctc aacaaacgcc agggtaaaca gttgctgacg gcattaggtt gcccggaaca
     1741 cctttataag aaagccccaa cggccgatct ggaagatgat cgcccttctc tgccagatga
     1801 agtggcactc ggcgtgacct atgacaatat cgacgactat ctggaaggga aaaacgtacc
     1861 tgaacaggtc gccagaacaa tagagaactg gtatctgaaa accgaacata aacgccgtcc
     1921 gccaattacc gttttcgatg atttctggaa aaagtaataa tttgcacata atggtttgtg
     1981 cgaaaaagag taatttgttc acgccggatg cggcgtgaac gccttattcg acctacaaaa
     2041 ctatgcaaat tcaatatatt gcaggagtcg aataggcctg acaggcgtag cacgtcagac
     2101 ggtgtaacct ttgtcatcga cccgcctctt ttttaatcgc ttcccgcctg ttacactgga
     2161 tagataacca gcattcggag tcaacagtgg tacggcgttt aacttctccg cggctcgaat
     2221 ttgaagctgc ggcaatttat gaatatcccg aacatttacg ttcattcctt aatgacttac
     2281 ccacccgacc aggggtgtat ctgtttcatg gcgaaagtga caccatgccg ctctatatcg
     2341 gcaaaagcgt taatatccgc agtcgggtgc tttctcattt acgcaccccg gatgaagccg
     2401 ccatgctacg gcaatcccgg cgaattagct ggatctgcac cgcaggcgaa atcggcgctc
     2461 tgctccttga agcgcgatta atcaaagaac aacagccgct gtttaataaa cggttgcgcc
     2521 gcaatcgcca gctctgtgcc ctgcaattaa atgaaaagcg cgtcgatgtg gtgtatgcca
     2581 aagaggtgga tttttcacga gcccccaacc tgtttggcct gtttgccaat aggcgcgcag
     2641 ctttgcaagc attgcagagc atcgctgatg aacaaaaact ttgttatggc ctgctgggac
     2701 tggaaccgtt aagtcgcggt cgtgcatgtt ttcgttcagc gctaaaacgt tgcgccggag
     2761 catgctgcgg taaagagagc catgaggaac atgcgctacg cttgcgccag tctctggagc
     2821 gtttgcgggt ggtgtgttgg ccttggcaag gggcggtggc gctgaaagaa cagcacccgg
     2881 aaatgactca atatcatatt attcaaaact ggctgtggct gggggcggtt aattcgctgg
     2941 aagacgcgac aacgttaatt cggacacccg ccgggtttga tcacgacggt tataaaattc
     3001 tttgtaagcc gctgctttcc ggtaactatg aaattactga acttgatccg gcgaatgacc
     3061 agcgagccag ttgatttcac tgaacaacag tttaccttgc ggttgtaaaa gccgcagggt
     3121 gtgcctgcca tcgaaccagc acgcgccctg gtcggtagtg agcagtttgt cgcccagttg
     3181 ccacgccccg ttaatcacaa acactacccc gccgcgtgag ccaaaggtag taaacgtacg
     3241 ttcggcaatt ctgactttcg ccttacagac atccaggcgc gtcataatat tgaaatccat
     3301 cgacatctgc cctgccgtca gtttcgcttt taccacctgg tccgccgcaa aggcaaaagg
     3361 ctgtaacggt tttaaggtat ggttaaagtg gtctgcgctt tcaaggaaca tctcgccgcc
     3421 ttccagcaac gtcactatcc tttccatacc gggaaacaaa gaaaactccc cattcgccgc
     3481 gatggatgca atgctggcac gccagtaaaa atcacgtttt gccggtggga aagtgcaaat
     3541 ttcgcgcgtt tcaccggcag catttcgcca cagattcacc gacattttac gcatatcaaa
     3601 gtattccata ctcgctcccg gcccgttctt ttcattgtta tcgttcaggt aaccgtggat
     3661 ggttatcggc aactcgggtg atttacgtta gtggtgatca ggcttttttt acaactgttt
     3721 gagaagagaa aagaaaaccg ccgatcctgt ccaccgcatt actgcaaggt agtggacaag
     3781 accggcggtc ttaagttttt tggctgaaag attattcagc agttgcaggc attttacctt
     3841 ttgccgctgg acgttctgtc agacgcttct caaaattagc attaaattgc tttttctgtt
     3901 ccggcgtcag gatgttgtaa attttgttct gggtttccat gtgcgccagc atgttagctt
     3961 tgcgctgttc ttccattttt gcgatctgcg cttcagcttt tgctttatcg aaggtatcgc
     4021 tggcaacgat gtcatgcatt gcgcggcgtt cttccagcgg cggacgtttc atctggtcac
     4081 gctggccttt catgatttcg cggatctgct gtttctgcgc gtcggtcagg ttcaggtctt
     4141 tgaacatcat gtcctgatgc ggaccgaact tgcctttgtg gtgcataatc ggcttcgcgt
     4201 cagccggtgc tgcggtagtg gtgtctgcgg catgggccag gttagccgcg ccaagagcca
     4261 gggtagaggc aacaaacagt gcagttaatt tacgcatatt ctatatcctt cctttcagtt
     4321 atttattacg gctttcttaa gtagcgtgcc gtgttgacga gattaacttt actgacttta
     4381 gcgtcaatta atcagagcaa cggtaaaaca atgaaagtgt aaaaaacact tttgcgccaa
     4441 ttatggagaa aaaaagaaaa tttgatggag agtgatgaga gaatattaca acacgatgat
     4501 tttgcagaga ttatgaagaa ctataccgga tg
//
LOCUS       DAZKHF020000299         4514 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2201S31241
            SAMN43267800-rid25106413.denovo.301, whole genome shotgun sequence.
ACCESSION   DAZKHF020000299 DAZKHF020000000
VERSION     DAZKHF020000299.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267800
            Sequence Read Archive: SRR30307263
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4514)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4514)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 4514)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350581.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350581.2
            Genome Coverage       :: 151x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:21:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,652
            CDSs (total)                      :: 5,557
            Genes (coding)                    :: 5,320
            CDSs (with protein)               :: 5,320
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 237
            CDSs (without protein)            :: 237
            Pseudo Genes (ambiguous residues) :: 0 of 237
            Pseudo Genes (frameshifted)       :: 102 of 237
            Pseudo Genes (incomplete)         :: 130 of 237
            Pseudo Genes (internal stop)      :: 52 of 237
            Pseudo Genes (multiple problems)  :: 41 of 237
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4514
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267800-rid25106413.denovo.301"
                     /serotype="O 26:H11"
                     /isolate="2201S31241"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-01"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            <1..92
                     /gene="kil"
                     /locus_tag="ACHOSC_RS21625"
                     /old_locus_tag="pgaptmp_004325"
     CDS             <1..92
                     /gene="kil"
                     /locus_tag="ACHOSC_RS21625"
                     /old_locus_tag="pgaptmp_004325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707273.1"
                     /note="Rac prophage Kil protein; Kil overexpression leads
                     to morphological defects and inhibits cell division;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="cell division protein FtsZ"
                     /protein_id="HGU4550128.1"
                     /translation="NLTTKTCISDCMIKVFLGRDGLPVKAESW"
     gene            92..262
                     /gene="ydaE"
                     /locus_tag="ACHOSC_RS21630"
                     /old_locus_tag="pgaptmp_004326"
     CDS             92..262
                     /gene="ydaE"
                     /locus_tag="ACHOSC_RS21630"
                     /old_locus_tag="pgaptmp_004326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdaE family protein"
                     /protein_id="HGU4550129.1"
                     /translation="MTKKIKCAYHLCKKDVEESKAIERMLHFMHGILSKDEPRKYCSE
                     ACAEKDQMAHEL"
     gene            337..612
                     /gene="racC"
                     /locus_tag="ACHOSC_RS21635"
                     /old_locus_tag="pgaptmp_004327"
     CDS             337..612
                     /gene="racC"
                     /locus_tag="ACHOSC_RS21635"
                     /old_locus_tag="pgaptmp_004327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein RacC"
                     /protein_id="HGU4550130.1"
                     /translation="MITNYEATVVTTDDIVHEVNLEGKRIGYVIKTENKETPFTVVDI
                     DGPSGNVKTLDEGVKKMCLVHIGKNLPAEKKAEFLATLIAMKLKGEI"
     gene            714..3314
                     /gene="recE"
                     /locus_tag="ACHOSC_RS21640"
                     /old_locus_tag="pgaptmp_004328"
     CDS             714..3314
                     /gene="recE"
                     /locus_tag="ACHOSC_RS21640"
                     /old_locus_tag="pgaptmp_004328"
                     /EC_number="3.1.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309961.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051908 - double-stranded DNA 5'-3' DNA
                     exonuclease activity [Evidence IEA];
                     GO_process: GO:0090304 - nucleic acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VIII"
                     /protein_id="HGU4550131.1"
                     /translation="MSTKPLFLLRKAKKSSGEPDVVLWASNDFESTCATLDYLIVKSG
                     KKLSSYFKAVATNFPVVNDLPAEGEIDFTWSERYQLSKDSMTWELKPGAAPDNAHYQG
                     NTNVNGEDMTEIEENMLLPISGQELPIRWLAQHGSEKPVTHVSRDGLQALHIARAEEL
                     PAVTALAVSHKTSLLDPLEIRELHKLVRDTDKVFPNPGNSNLGLITAFFEAYLNADYT
                     DRGLLTKEWMKGNRVSHITRTASGANAGGGNLTDRGEGFVHDLTSLARDVATGVLARS
                     MDLDIYNLHPAHAKRIEEIIAENKPPFSVFRDKFITMPGGLDYSRAIVVASVKEAPIG
                     IEVIPAHVTEYLNKVLTETDHANPDPEIVDIACGRSSAPMPQRVTEEGKQDYEEKPQP
                     SGTTAVEQGEAETMEPDATEHHQDTQPLDAQSQVNSVDAKYQELRAELHEARKNIPSK
                     NPVDADKLLAASRGEFVDGISDPNDPKWVKGIQTRDCVYQNQPETEKTSPDMNQPEPV
                     VQQEPEIACNACGQTGGDNCPDCGAVMGDATYQETFDEESQVEAKENDPEEMEGAEHP
                     HNENAGSDPHRDCSDETGEVADPVIVEDIEPGIYYGISNENYHAGPGVSKSQLDDIAD
                     TPALYLWRKNAPVDTTKTKTLDLGTAFHCRVLEPEEFSNRFIVAPEFNRRTNSGKEEE
                     KAFLRECASTGKTVITAEEGRKIELMYQSVMALPLGQWLVESAGHAESSIYWEDPETA
                     ILCRCRPDKIIPEFHWIMDVKTTADIQRFKTAYYDYRYHVQDAFYSDGYEAQFGVQPT
                     FVFLVASTTIECGRYPVEIFMMGEEAKLAGQLEYHRNLRTLADCLNTDEWPAIKTLSL
                     PRWAKEYAND"
     gene            3307..4116
                     /gene="recT"
                     /locus_tag="ACHOSC_RS21645"
                     /old_locus_tag="pgaptmp_004329"
     CDS             3307..4116
                     /gene="recT"
                     /locus_tag="ACHOSC_RS21645"
                     /old_locus_tag="pgaptmp_004329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006259 - DNA metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombination protein RecT"
                     /protein_id="HGU4550132.1"
                     /translation="MTKQPPIAKADLQKTQGNRAPAAIKNNDVISFINQPSMKEQLAA
                     ALPRHMTAERMIRIATTEIRKVPALGNCDTMSFVSAIVQCSQLGLEPGSALGHAYLLP
                     FGNKNEKSGKKNVQLIIGYRGMIDLARRSGQIASLSARVVREGDEFNFEFGLDEKLIH
                     RPGENEDAPVTHVYAVARLKDGGTQFEVMTRKQIELVRSQSKAGNNGPWVTHWEEMAK
                     KTAIRRLFKYLPVSIEIQRAVSMDEKEPLTIDPADSSVLTGEYSVIDNSEE"
     gene            4173..4322
                     /gene="ralR"
                     /locus_tag="ACHOSC_RS21650"
                     /old_locus_tag="pgaptmp_004330"
     CDS             4173..4322
                     /gene="ralR"
                     /locus_tag="ACHOSC_RS21650"
                     /old_locus_tag="pgaptmp_004330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system
                     endodeoxyribonuclease toxin RalR"
                     /protein_id="HGU4550133.1"
                     /translation="MRYDNVKPCPFCGCPSVTVKAISGYYRAKCNGCESRTGYGGSEK
                     EALER"
     gene            4360..>4514
                     /gene="rcbA"
                     /locus_tag="ACHOSC_RS21655"
                     /old_locus_tag="pgaptmp_004331"
     CDS             4360..>4514
                     /gene="rcbA"
                     /locus_tag="ACHOSC_RS21655"
                     /old_locus_tag="pgaptmp_004331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="double-strand break reduction protein RcbA"
                     /protein_id="HGU4550134.1"
                     /translation="MYKITATIEKEGGTPTNWTRYSKSKLTKSECEKMLSGKKEAGVS
                     REQKVKL"
BASE COUNT         1406 a         1024 c         1080 g         1004 t
ORIGIN      
        1 gtaacctgac tacaaaaaca tgcatttctg actgcatgat taaagtcttc ctcggtcgtg
       61 atggtttacc tgtaaaggcg gagtcatggt gatgactaag aaaataaaat gtgcttacca
      121 cctttgcaaa aaagacgttg aagaaagcaa agctattgaa agaatgcttc acttcatgca
      181 cgggatttta tcaaaagacg aaccgagaaa atattgcagt gaagcttgtg ccgaaaaaga
      241 ccagatggca catgaacttt aattaattga ctattcgaaa ctgaatttat gccagaaatg
      301 gcaggtattc gctcaacctt aattaaggag aaaaacatga ttaccaatta tgaagccact
      361 gttgtaacta ccgatgacat tgttcacgag gtgaatctgg aaggaaagcg cattggctac
      421 gtaattaaaa cagaaaataa agaaacccca ttcactgtgg ttgatatcga tggtccatca
      481 ggcaacgtaa aaacacttga tgaaggtgtc aaaaaaatgt gcctggtgca tatcggaaag
      541 aatctgcccg cagaaaaaaa agccgaattt ctggcaactc taattgcaat gaaattaaaa
      601 ggtgaaatct gaaagaaata gcctgcgtat ggcgcaggct atgaacagtg tgtatccggc
      661 aagatcattc actgaacaaa acgaatttta atctgagttg aggttaaaaa acaatgagca
      721 caaaaccact cttcctgtta cggaaagcga aaaaatcatc cggtgaacct gacgtcgtcc
      781 tgtgggcaag caacgatttt gaatcgacct gtgccactct ggactacctg atcgttaagt
      841 caggtaaaaa actgagcagc tattttaaag ctgttgccac gaattttcct gtcgttaatg
      901 acctgcccgc tgaaggtgag atcgatttta cctggagtga acgctatcaa ctcagcaaag
      961 actccatgac atgggaacta aaaccgggag cagcaccaga caacgctcac tatcaaggca
     1021 ataccaacgt caacggcgaa gacatgactg agattgagga gaatatgcta ctcccaattt
     1081 ctggccagga actgcccatt cgttggcttg ctcaacacgg cagcgaaaaa ccggtaacgc
     1141 acgtttcacg cgacggactc caggcattac acattgctcg ggctgaagaa ctaccggctg
     1201 ttactgccct ggctgtttcc cacaaaacca gcctgctcga cccgctggaa attcgcgaac
     1261 tccacaaact ggttcgtgac actgacaaag ttttccctaa tcctggtaat tcaaacctgg
     1321 gactgataac tgcttttttc gaagcatacc tgaacgctga ctacaccgat cgaggactgc
     1381 tgacaaaaga gtggatgaag ggtaatcgtg tttcacacat cactcgcacg gcttccggtg
     1441 ctaatgctgg cggcggaaac ctcaccgatc gcggcgaagg tttcgtacac gatctgacgt
     1501 cactggcgcg ggacgtagcc actggcgtac tggcccgttc aatggatctg gacatctata
     1561 accttcatcc ggcacacgct aaacgcattg aggaaattat cgctgaaaat aaaccgccct
     1621 tttctgtttt ccgcgacaaa ttcatcacca tgcctggcgg gctggattat tcccgcgcca
     1681 tcgtggttgc gtccgtaaaa gaagcaccaa ttgggatcga ggtcatcccc gcgcacgtca
     1741 ctgaatatct gaacaaagta ctgactgaaa ccgatcatgc caaccctgat ccggaaatcg
     1801 tggatattgc ctgcggtcgc tcctctgccc cgatgccgca gcgagtaaca gaagaaggaa
     1861 aacaggatta tgaagaaaaa ccgcaaccat ctggaacaac ggcagttgaa cagggagagg
     1921 ctgaaacaat ggaaccggac gcaactgaac atcatcagga cacgcagccg ctggatgctc
     1981 agtcacaggt aaattctgtt gatgcgaaat atcaggaact gcgggcagaa ctccatgaag
     2041 cccggaaaaa cattccatca aaaaatcctg tcgatgccga taaattgctt gctgcatcac
     2101 gtggtgaatt tgttgacgga attagcgacc cgaacgatcc gaaatgggta aaggggatcc
     2161 agactcgcga ttgtgtgtac cagaaccagc cagaaacgga aaaaaccagc ccggatatga
     2221 atcaacctga gccagtagtg caacaggaac cggaaatagc ctgcaatgcc tgcggccaga
     2281 ctggcgggga taactgccct gactgtggtg cggtgatggg cgacgcaaca taccaggaaa
     2341 cattcgatga agagagtcag gttgaagcta aggaaaatga tccggaggaa atggaaggcg
     2401 ctgaacatcc gcacaatgag aatgctggca gcgatccgca tcgcgattgc agtgatgaaa
     2461 ctggcgaagt cgcagatccc gtaatcgtag aagacataga gccaggtatt tattacggaa
     2521 tttcgaatga gaattaccac gcgggtcccg gtgtcagtaa gtctcagctc gatgacattg
     2581 ctgatactcc ggcactgtat ttgtggcgta aaaatgcccc cgtggacacc acaaagacaa
     2641 aaacgctcga tttaggaacc gctttccact gccgggtact tgagccggaa gaattcagta
     2701 accgctttat cgtagcacct gaatttaacc gccggacaaa ctccggaaaa gaagaagaga
     2761 aagcgtttct gagggaatgc gcaagcacag gaaaaacggt tatcactgcc gaagaaggcc
     2821 ggaaaattga actcatgtat cagagcgtta tggctttgcc gctggggcaa tggcttgttg
     2881 aaagcgccgg acacgctgaa tcatcaattt actgggaaga tcctgaaaca gcaattttgt
     2941 gtcggtgccg tccggacaaa attatccctg aatttcactg gatcatggac gtgaaaacta
     3001 cggcggatat tcaacgattc aaaaccgctt attacgacta ccgctatcac gttcaggatg
     3061 cattctacag tgacggttat gaagcacagt ttggagtgca gccaactttc gtttttctgg
     3121 ttgccagcac aactattgaa tgcggacgtt atccggttga aattttcatg atgggcgaag
     3181 aagcaaaact ggcaggtcag ctggaatatc accgcaatct gcgaaccctg gctgactgcc
     3241 tcaataccga tgaatggcca gctattaaga cgttatcact gccccgctgg gctaaggaat
     3301 atgcaaatga ctaagcaacc accaatcgca aaagccgatc tgcaaaaaac tcagggaaac
     3361 cgtgcaccag cagcaattaa aaataacgac gtgattagtt ttattaacca gccatcaatg
     3421 aaagagcaac tggcagcagc tcttccacgc catatgacgg ctgaacgtat gatccgtatc
     3481 gccaccacag aaattcgtaa agttccggcg ttaggaaact gtgacactat gagttttgtc
     3541 agtgcaatcg tacagtgttc acagctcgga cttgagcccg gtagcgccct cggtcatgca
     3601 tatttactgc cttttggtaa taaaaacgaa aagagcggta aaaaaaacgt tcagctaatc
     3661 attggctatc gcggcatgat tgatctggct cgccgttcag gtcaaatcgc cagcctgtca
     3721 gcccgtgttg tccgtgaagg tgacgagttt aatttcgaat ttggccttga tgaaaagtta
     3781 atacaccgcc caggagaaaa cgaagatgcc cctgttaccc acgtctatgc tgtcgcaaga
     3841 ctgaaagacg gaggtactca gtttgaagtt atgacgcgca aacagattga gctggtgcgc
     3901 agccagagta aagctggtaa taacgggccg tgggtaactc actgggaaga aatggcaaag
     3961 aaaacggcta ttcgtcgcct gttcaaatat ctgcccgtat caattgagat ccagcgtgca
     4021 gtatcaatgg atgaaaagga accactgaca atcgatcctg cagattcctc tgtattaacc
     4081 ggggaataca gtgtaatcga taattcagag gaataattca gcctggcggt gtaatgcacc
     4141 gccaacttga gacagttttt atgagaaaaa ttatgagata tgacaatgtt aaaccatgtc
     4201 cattttgtgg ttgtccatca gtaacggtga aagccatttc aggatattac cgagcgaagt
     4261 gtaacggatg cgaatcccga accggttatg gtggaagtga aaaagaagca ctcgaacgat
     4321 gaaataaacg aaccactgga aataataatg gaggtgttca tgtataaaat taccgccact
     4381 attgaaaagg aaggtggcac tcctactaac tggacaagat attcaaaatc taaactaacg
     4441 aaatcagaat gcgaaaaaat gctctcaggt aaaaaagaag caggcgtttc cagagagcag
     4501 aaagtaaaac tgat
//
LOCUS       DAZKHF020000300         4437 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2201S31241
            SAMN43267800-rid25106413.denovo.302, whole genome shotgun sequence.
ACCESSION   DAZKHF020000300 DAZKHF020000000
VERSION     DAZKHF020000300.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267800
            Sequence Read Archive: SRR30307263
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4437)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4437)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 4437)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350581.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350581.2
            Genome Coverage       :: 151x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:21:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,652
            CDSs (total)                      :: 5,557
            Genes (coding)                    :: 5,320
            CDSs (with protein)               :: 5,320
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 237
            CDSs (without protein)            :: 237
            Pseudo Genes (ambiguous residues) :: 0 of 237
            Pseudo Genes (frameshifted)       :: 102 of 237
            Pseudo Genes (incomplete)         :: 130 of 237
            Pseudo Genes (internal stop)      :: 52 of 237
            Pseudo Genes (multiple problems)  :: 41 of 237
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4437
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267800-rid25106413.denovo.302"
                     /serotype="O 26:H11"
                     /isolate="2201S31241"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-01"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            127..1284
                     /gene="acrE"
                     /locus_tag="ACHOSC_RS21660"
                     /old_locus_tag="pgaptmp_004332"
     CDS             127..1284
                     /gene="acrE"
                     /locus_tag="ACHOSC_RS21660"
                     /old_locus_tag="pgaptmp_004332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter periplasmic
                     adaptor subunit AcrE"
                     /protein_id="HGU4550135.1"
                     /translation="MTKHARFFLLPSFILISAALIAGCNDKGEEKAHVGEPQVTVHIV
                     KTAPLEVKTELPGRTNAYRIAEVRPQVSGIVLNRNFTEGSDVQAGQSLYQIDPATYQA
                     NYDSAKGELAKSEAAAAIAHLTVKRYVPLVGTKYISQQEYDQAIADARQADAAVIAAK
                     ATVESARINLAYTKVTAPISGRIGKSTVTEGALVTNGQTTELATVQQLDPIYVDVTQS
                     SNDFMRLKQSVEQGNLHKENATSNVELVMENGQTYPLKGTLQFSDVTVDESTGSITLR
                     AVFPNPQHTLLPGMFVRARIDEGVQPDAILIPQQGVSRTPRGDATVLIVNDKSQVEAR
                     PVVASQAIGDKWLISEGLKSGDQVIVSGLQKARPGEQVKATTDTPADTASK"
     gene            1296..4389
                     /gene="acrF"
                     /locus_tag="ACHOSC_RS21665"
                     /old_locus_tag="pgaptmp_004333"
                     /pseudo
     CDS             1296..4389
                     /gene="acrF"
                     /locus_tag="ACHOSC_RS21665"
                     /old_locus_tag="pgaptmp_004333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417732.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter permease
                     subunit AcrF"
BASE COUNT         1069 a         1083 c         1181 g         1104 t
ORIGIN      
        1 ttcataacta ttccttacat cgacgaatga taatttgtag gatagcaaac tgtatttttc
       61 tttctgcgag ttaacgcgtt acctttctgg gtaaataacg cgcttttggt tttttgagga
      121 atagtaatga cgaaacatgc caggtttttc ctcctgccct cctttattct gatctccgcg
      181 gctttaatcg ccggttgtaa cgataaggga gaagagaaag ctcacgtcgg tgaaccgcag
      241 gttaccgttc atattgtgaa aacggccccg ttagaagtta agactgaatt accaggccgc
      301 accaatgctt accgtatagc cgaagttcgc ccacaggtta gcgggatcgt actgaatcgt
      361 aatttcactg aaggcagcga tgtacaagca ggccagtccc tgtaccagat cgatcccgcg
      421 acctatcagg caaattatga cagcgcgaaa ggcgaactgg cgaaaagtga agccgccgcc
      481 gccatcgcgc atttgacggt aaaacgttac gttccgctcg tgggtacgaa atacatcagc
      541 cagcaggagt acgaccaggc cattgctgat gctcgccagg ccgatgccgc cgtgattgcc
      601 gcaaaagcca cagtcgaaag cgctcgcatc aatctcgctt ataccaaagt gactgcgcca
      661 attagcggac gtatcggcaa atcgaccgtg accgaaggcg ctcttgtcac taatgggcaa
      721 acgactgaac tggcgactgt ccagcagctc gatcctatct acgttgatgt gacccaatcc
      781 agcaacgatt ttatgaggct gaagcaatcc gtagagcaag gaaatttgca taaggaaaac
      841 gccaccagca acgtagagtt ggtcatggaa aacggtcaaa cctatcccct gaaaggtacg
      901 ctgcaattct ctgatgtgac cgttgatgaa agcaccggct ccataaccct acgtgctgtc
      961 ttccctaacc cgcaacatac gcttttgccg ggtatgtttg tgcgtgcacg gattgatgaa
     1021 ggcgtccaac ctgacgccat tcttatcccg caacaaggcg ttagccgcac accgcgtggt
     1081 gatgcaaccg tgctgattgt taacgataaa agtcaggtgg aagcgcgccc tgtcgttgcc
     1141 agtcaggcga ttggcgataa atggttgatt agcgaaggtc tgaaatctgg cgatcaagtc
     1201 attgtcagcg gcctgcaaaa agcgcgtccg ggagagcagg ttaaagccac taccgatacc
     1261 cccgcagata ctgcatcgaa gtaaggtaat ctgacatggc aaactttttt attcgacgac
     1321 cgatatttgc atgggtgctg gccattattc tgatgatggc gggcgcactg gcgatcctac
     1381 aattgcccgt cgctcagtat ccaaccattg caccgcctgc ggtttctgtt tcggcaaact
     1441 atccaggtgc tgatgcgcag accgtgcagg atacggtgac gcaggttatc gaacagaata
     1501 tgaatggtat cgataacctg atgtatatgt cctccaccag cgattccgcc ggtagcgtga
     1561 caattaccct taccttccag tccgggaccg atcctgatat cgcgcaagtg caggtgcaga
     1621 acaaactcca gctcgccacg ccgttgctgc cgcaggaggt tcagcagcag gggatcagtg
     1681 ttgaaaagtc cagtagcagc tatttgatgg tggcgggctt tgtctctgat aacccagaca
     1741 ccacacagga cgatatctcg gactatgtgg cctctaacgt taaagatacg cttagccgtc
     1801 tgaatggcgt cggtgacgta cagcttttcg gcgcacagta tgcgatgcgt atctggctgg
     1861 acgccgatct gctaaacaaa tataaactga caccggttga tgtgattaac cagttgaagg
     1921 tacagaacga tcagatcgct gccggacagt tgggcggaac gccagcgtta ccagggcaac
     1981 agttgaatgc ctcgattatt gctcaaacgc ggttaaaaaa tccggaagaa ttcggcaaag
     2041 tgaccctgcg cgtaaacagt gacggctcgg tggtacgcct gaaagatgtc gcacgggttg
     2101 aacttggcgg tgaaaactat aacgttatcg ctcgtatcaa cggaaaaccg gcggcgggcc
     2161 tggggattaa gctggcaacc ggcgcgaatg ctctcgatac cgcgaaagcc attaaggcaa
     2221 aactggcgga attacagcca ttcttcccgc agggaatgaa ggttctctac ccttatgaca
     2281 ccacgccatt cgtccagctt tctattcacg aagtggtaaa aacgctgttc gaagccatta
     2341 tgctggtgtt cctggtgatg tatctgttct tgcagaatat gcgagcaacg ctgatcccca
     2401 ccattgcggt acccgtggtg ttgttaggga cgtttgccat cctcgccgct tttggttact
     2461 ccatcaacac actaacgatg ttcgggatgg tgcttgccat cgggctgctc gtcgatgatg
     2521 cgatagtggt ggtggagaac gtcgagcgcg tgatgatgga ggataagctc ccgccaaaag
     2581 aagcgacgga aaaatcaatg tcgcaaatcc agggcgcact ggtgggtatc gcgatggtgc
     2641 tgtcagcggt atttattccg atggcattct tcggcggttc tactggggca atttatcgcc
     2701 agttctctat caccatcgtt tcggcaatgg cgctttctgt tctggtggca ttgattctta
     2761 cccctgcgtt atgtgcaacg ctgcttaaac ccgtctctgc tgagcatcac gaaaataagg
     2821 gcggtttctt cggctggttt aatactacct tcgatcatag cgttaaccac tacaccaaca
     2881 gcgtcggcaa aatcctcggt tccacaggac gatattgcgc tgattgttgc aggaatggtg
     2941 gtgttgtttt tacgtcttcc gtcttccttc ttacctgaag aggatcaggg tgtctttctg
     3001 accatgattc agttacccgc tggcgcgacg caagagcgga cgcaaaaagt gttggatcaa
     3061 gtgacggatt actatctgaa gaacgagaaa gcgaacgttg aaagtgtctt tacggttaac
     3121 ggctttagct tcagcggcca ggcacaaaac gccggtatgg ccttcgtcag tctgaaaccg
     3181 tgggaagagc gtaatggtga tgaaaacagt gcggaagcgg taatccatcg tgccaaaatg
     3241 gaatttggca agatccgcga cggttttgtc attccattca atatgccagc cattgttgaa
     3301 ctgggcacgg caacgggttt cgactttgag ttaattgatc aggctgggct gggtcacgat
     3361 gccctaaccc aggcccgtaa ccagttgctt ggtatggcgg cgcaacatcc tgccagctta
     3421 gtcagcgtgc gtcctaatgg cctggaagac accgcgcagt ttaaactgga agttgaccag
     3481 gaaaaggcgc aggcattagg tgtttcactt tctgacatca atcagaccat ttcaacggcg
     3541 ttgggtggga cttacgttaa cgacttcatc gaccgtggtc gcgtgaaaaa ggtgtatgtt
     3601 caggcggatg ccaaattccg tatgctgcca gaagatgtcg ataaacttta tgtccgcagc
     3661 gccaacggcg aaatggtgcc attctcggcc tttaccactt cacattgggt gtatggctct
     3721 ccgcgactgg aacgctacaa cggtctgccg tcaatggaga ttcaggggga agccgcgcca
     3781 ggaaccagtt ccggcgatgc catggcgttg atggaaaacc ttgcgtcaaa attacctgct
     3841 ggcattggtt atgactggac gggtatgtcg tatcaggaac gcttatcggg aaaccaggct
     3901 cccgctctgg tagcaatttc ctttgtggtt gttttcctgt gccttgctgc actctatgaa
     3961 agctggtcaa ttcctgtctc ggttatgttg gtcgtgccgt tagggattgt cggcgtgctg
     4021 ctggcggcga cactctttaa tcaaaaaaat gacgtctact ttatggtggg cttgctaacg
     4081 acaattggct tgtcggccaa aaacgctatt ttgattgttg agttcgctaa agatctcatg
     4141 gagaaagagg gtaaaggtgt tgttgaagcg acactaatgg cagtacgtat gcgtctgcgt
     4201 cctatcctga tgacctctct cgcctttatt ctcggcgtat taccgctagc tatcagtaac
     4261 ggtgccggca gtggcgcgca gaacgcagtg ggtatcgggg taatgggcgg aatggtctct
     4321 gcaacgttgc tggcaatctt cttcgtaccg gtgttctttg tggtgatccg ccgttgcttt
     4381 aaaggataaa tcagaaacat aaaggcactt tcgggtgcct ttattatttt cagtgaa
//
LOCUS       DAZKHF020000301         4424 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2201S31241
            SAMN43267800-rid25106413.denovo.303, whole genome shotgun sequence.
ACCESSION   DAZKHF020000301 DAZKHF020000000
VERSION     DAZKHF020000301.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267800
            Sequence Read Archive: SRR30307263
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4424)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4424)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 4424)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350581.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350581.2
            Genome Coverage       :: 151x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:21:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,652
            CDSs (total)                      :: 5,557
            Genes (coding)                    :: 5,320
            CDSs (with protein)               :: 5,320
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 237
            CDSs (without protein)            :: 237
            Pseudo Genes (ambiguous residues) :: 0 of 237
            Pseudo Genes (frameshifted)       :: 102 of 237
            Pseudo Genes (incomplete)         :: 130 of 237
            Pseudo Genes (internal stop)      :: 52 of 237
            Pseudo Genes (multiple problems)  :: 41 of 237
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4424
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267800-rid25106413.denovo.303"
                     /serotype="O 26:H11"
                     /isolate="2201S31241"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-01"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            153..4406
                     /gene="fdeC"
                     /locus_tag="ACHOSC_RS21670"
                     /old_locus_tag="pgaptmp_004334"
     CDS             153..4406
                     /gene="fdeC"
                     /locus_tag="ACHOSC_RS21670"
                     /old_locus_tag="pgaptmp_004334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000092554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inverse autotransporter adhesin FdeC"
                     /protein_id="HGU4550136.1"
                     /translation="MSHYKTGHKQPRFRYSVLTRCVAWANISVQVLFPLAVTFTPVMA
                     ARAQHAVQPRLSMGNTTVTADNNVEKNVASFAANAGTFLSSQPDSDATRNFITGMATA
                     KANQEIQEWLGKYGTARVKLNVDKDFSLKDSSLEMLYPIYDTPTNMLFTQGAIHRTDD
                     RTQSNIGFGWRHFSGNDWMAGVNTFIDHDLSRSHTRIGVGAEYWRDYLKLSANGYIRA
                     SGWKKSPDVEDYQERPANGWDIRAEGYLPAWPQLGASLMYEQYYGDEVGLFGKDKRQK
                     DPHAISAEVTYTPVPLLTLSAGHKQGKSGENDTRFGLEVNYRIGEPLAKQLDTDSIRE
                     RRVLAGSRYDLVERNNNIVLEYRKSEVIRIALPERIEGKGGQTLSLGLVVSKATHGLK
                     NVQWEAPSLLAEGGKITGQGSQWQVTLPAYRPGKDNYYAISAVAYDNKGNASKRVQTE
                     VVITGAGMSADRTALTLDGQSRIQMLANGNEQRPLVLSLRDAEGQPVTGMKDQIKTEL
                     AFKPAGNIVTRSLKATKSQAKPTLGEFTETEAGVYQSVFTTGTQSGEATITVSVDGMS
                     KTVTAELRATMMDVANSTLSANEPSGDVVADGQQAYTLTLTAVDSEGNPVTGEASRLR
                     FVPQDTNGVTVGAISEIKPGVYSATVSSTRAGNVVVRAFSEQYQLGTLQQTLKFVAGP
                     LDAAHSSITLNPDKPVVGGTVTAIWTAKDANDNPVTGLNPDAPSLSGAAAVGSTASGW
                     TDNGDGTWTAQISLGTTAGELEVMPKLNGQDAAANAAKVTVVADALSSGQSTVSVAAD
                     RVKAGESTTVTLIAKDAHGNAISGLSLSASLTGAASEGATVSGWTEKGDGSYVATLTT
                     GGKTGELLVMPLFNGQPAATEAAQLTVIAGEMSSANSTLVADNKAPTVKTTTKLTFTV
                     KDAYGNLVTGLKPDAPQFSGAASTGTERPSTGDWTETSNGVYVATLTLGSAAGQLSVM
                     PRVNGQNAVAQPLVLNVAGDASKAEIRDMTVKVDNQLANGQSTNQVTLTVVDTYGNPL
                     QGQNVTLTLPKGVTSKTGNTVTTDAAGKADIELMSTVAGEHSITASVNNAQKTVTVKF
                     NADASTGQANLQVDTAAQKVANGKDAFTLTANVEDKNGNPVPGSLVTFNLPRGVKPLT
                     GDNVWVKANDEGKAELQVVSVTAGTYEITASAGNSQPSNTQTITFVADKATATVSGIE
                     VMGNYALADGKAKQTYKVTVTDANNNLVKDSEVTLTASPASLNLEPNGTATTNEQGQA
                     IFTATTTVAATYTLKAQVSQTNGQVSTKTAESKFVADDKNAVLTASSDMQSLVADGKS
                     TAKLEVTLMSANNPVGGNMWVDIQTPEGVTEKDYQFLPSKNDHFVSGKITRKFSTSKP
                     GVYTFTFNALTYGGYEMKPVTVTITAVDADTAKDEEAMK"
BASE COUNT         1154 a         1037 c         1254 g          979 t
ORIGIN      
        1 ggatatatta ggtggcggtg cactttctta cataaaggta tttccttttc tgcggaaaag
       61 gaaatcggga aatccccggt ttttctgaca agcagacgcc attatttgtg tctgcctatg
      121 ttcgttaatt cgttcatcag gaaattatct caatgtcaca ttataaaaca ggtcataaac
      181 aaccacgatt tcgttattca gttctgaccc gctgcgtggc gtgggcaaat atctctgttc
      241 aggttctttt tccactcgct gtcaccttta ccccagtaat ggcggcacgt gcgcagcatg
      301 cggttcagcc acggttgagc atgggaaata ctacggtaac tgctgataat aacgtggaga
      361 aaaatgtcgc gtcgtttgcc gcaaatgccg ggacattttt aagcagtcag ccagatagcg
      421 atgcgacacg taactttatt accggaatgg ccacagctaa agctaaccag gaaatacagg
      481 agtggctcgg gaaatatggt actgcgcgcg tcaaactgaa tgtcgataaa gatttctcgc
      541 tgaaggattc ttcgctggaa atgctttatc cgatttatga tacgccaaca aatatgttgt
      601 tcactcaggg agcaatacat cgtaccgacg atcgtactca gtcaaatatt ggttttggct
      661 ggcgtcattt ttcaggaaat gactggatgg cgggggtgaa cacctttatc gaccatgatt
      721 tatcccgtag tcatacccgc attggtgttg gtgcggaata ctggcgcgat tatctgaaac
      781 tgagcgccaa tggttatatc cgggcttctg gctggaaaaa atcgccggat gttgaggatt
      841 atcaggaacg cccggcgaat ggctgggata ttcgtgctga gggctattta cccgcctggc
      901 cgcagcttgg cgcaagcctg atgtatgaac agtattatgg cgatgaagtc gggctgtttg
      961 gtaaagataa gcgccagaaa gacccgcatg ctatttctgc cgaggtgacc tatacgccag
     1021 tgcctcttct gacactgagc gccgggcata agcagggcaa gagtggtgag aatgacactc
     1081 gctttggcct ggaagttaat tatcggattg gcgaacctct ggcgaaacaa ctcgatacag
     1141 acagcattcg cgagcgtcgg gtactggcag gcagccgcta tgacctggtt gagcgtaata
     1201 acaacatcgt tcttgagtac cgcaaatctg aagtgatccg tattgctctg cctgaacgta
     1261 ttgaaggtaa gggtggtcag acactttccc tggggcttgt ggtcagcaaa gcaactcacg
     1321 gactgaaaaa tgtgcagtgg gaagcgccgt cattactggc tgagggtggc aaaattaccg
     1381 gtcagggtag tcagtggcaa gtaacgctcc cggcttatcg tccaggcaaa gacaattatt
     1441 atgcgatttc tgcggttgcc tacgataaca aaggcaatgc ctcaaaacgc gtgcagacag
     1501 aggtggtcat taccggagca ggtatgagcg ccgatcgcac ggcgttaacg cttgacggtc
     1561 agagccgtat tcaaatgctt gctaacggta atgagcaaag accgctggtg ctgtctctgc
     1621 gcgacgccga ggggcagcca gtcacgggca tgaaagatca gatcaagact gaactagcct
     1681 tcaaaccggc tggaaatatt gtgactcgtt ccctaaaggc cactaaatca caggcaaagc
     1741 caacactggg tgagttcacc gaaactgaag caggggtgta tcagtctgtc tttactaccg
     1801 gaacgcagtc aggtgaggca acgattactg ttagcgttga tggcatgagc aaaaccgtca
     1861 ctgcagaact gcgggccacg atgatggatg tggcaaactc caccctgagc gctaacgagc
     1921 cgtcaggtga tgtggttgct gatggtcagc aagcctatac gttgacgttg actgcggtgg
     1981 actccgaggg taatccggtg acgggagaag ccagccgctt gcgatttgtt ccgcaagaca
     2041 ctaatggtgt aaccgttggt gccatttcgg aaataaaacc aggcgtttac agcgccacgg
     2101 tttcttcaac ccgtgccgga aacgttgttg tgcgtgcttt cagcgagcag tatcagctgg
     2161 gcacattaca acaaacgctg aagtttgttg ccgggccgct tgatgcagca cattcgtcca
     2221 tcacactgaa tcctgataaa ccggtggttg gcggtacagt tacggcaatc tggacggcaa
     2281 aagatgctaa tgacaaccct gtaactggtc tcaatccgga tgcaccgtca ttatcgggcg
     2341 cagctgctgt aggttctacg gcatcaggct ggacggataa tggcgacggg acctggactg
     2401 cgcagatttc tctcggcact acggcgggtg aattagaggt tatgccgaag ctaaatggac
     2461 aggatgcggc agcgaatgcg gctaaagtaa ccgtggtggc tgatgcgtta tcttcaggcc
     2521 agtcgacagt gtctgtagcc gcagatcgcg taaaagcagg cgaaagcaca accgtgacgc
     2581 ttattgcaaa agatgcacat ggcaacgcta tcagtggtct ttccctgtcg gcaagcctga
     2641 cgggtgctgc gtctgaaggg gcgactgttt ctggttggac cgaaaaaggt gatggttcct
     2701 atgtcgctac gctgacaaca ggtggaaaga cgggtgagct tctcgtcatg ccgctattca
     2761 acggccagcc agcagccacc gaagccgcgc agttgactgt cattgcgggg gagatgtcat
     2821 cagcgaactc tacgcttgtt gctgacaata aggctccgac cgtcaaaacg acgacgaaac
     2881 tcaccttcac cgtgaaggat gcgtacggga accttgtcac cgggctgaag ccagatgcac
     2941 cgcagtttag tggtgccgcc agcacgggga cagagcgacc ttcaacagga gactggacag
     3001 aaacaagtaa tggggtctac gtggcgacct taacgctggg atctgccgcg ggccagttgt
     3061 ctgtgatgcc gcgagtgaac ggccaaaatg ccgttgctca gccactggtg ctgaatgttg
     3121 ctggtgacgc atctaaggct gagattcgtg atatgacggt gaaggttgat aaccagctgg
     3181 ctaatggaca atcgactaac caggtaaccc tgaccgttgt ggacacctat ggtaacccgt
     3241 tgcagggaca aaatgtgacg ctgactctgc cgaaaggtgt gaccagcaag acggggaata
     3301 cggtaacaac cgatgcggca ggtaaagccg acattgagct gatgtcaacg gttgccgggg
     3361 aacacagcat cacggcctca gtgaataatg ctcagaagac ggtcacggtg aaattcaacg
     3421 cggatgccag caccggtcag gcaaacctgc aggtagacac cgctgctcaa aaagtggcaa
     3481 acggcaaaga tgcctttacg ctgacggcga acgttgagga taaaaatggt aaccctgttc
     3541 cagggagcct ggtgaccttt aatctgcccc ggggtgtcaa gccgcttaca ggcgataatg
     3601 tctgggtgaa agccaacgat gaggggaaag ccgagttgca ggtggtttca gtgactgccg
     3661 gaacgtatga gatcacggca tcggcaggga atagccagcc ttcgaatacg cagactataa
     3721 cgtttgtagc cgataaggct accgcaaccg tctccggtat tgaggtgatg ggcaactatg
     3781 cgctggcgga cggcaaagcc aaacaaacgt ataaagttac ggtgactgat gccaataaca
     3841 atttggtgaa agatagcgaa gtgacgctga ctgccagccc ggcttcgtta aacctggaac
     3901 cgaatggcac tgcgacaacg aatgagcaag ggcaggctat tttcaccgct accactactg
     3961 ttgcggcgac atacacactc aaggcgcaag tgagtcagac caacggtcag gtatcaacga
     4021 aaactgccga atctaaattc gttgcggatg ataaaaacgc ggtactcacc gcatcatctg
     4081 atatgcaatc tctggtggcg gatgggaaat cgactgcgaa gctggaggtg acactgatgt
     4141 cggcaaacaa ccccgttggc gggaatatgt gggtcgacat tcagacgccg gaaggggtga
     4201 cggaaaagga ttatcagttc ctgccgtcga aaaatgacca tttcgtgagc ggaaaaatca
     4261 cgcgtaaatt tagtaccagc aagcctggtg tctatacgtt cacatttaac gccctgacct
     4321 atggcgggta cgaaatgaag ccagtgacgg tgaccattac cgcggtggat gccgatacgg
     4381 caaaggacga ggaggcgatg aaataatttt aagaatatga aagt
//
LOCUS       DAZKHF020000302         4407 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2201S31241
            SAMN43267800-rid25106413.denovo.304, whole genome shotgun sequence.
ACCESSION   DAZKHF020000302 DAZKHF020000000
VERSION     DAZKHF020000302.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267800
            Sequence Read Archive: SRR30307263
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4407)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4407)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 4407)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350581.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350581.2
            Genome Coverage       :: 151x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:21:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,652
            CDSs (total)                      :: 5,557
            Genes (coding)                    :: 5,320
            CDSs (with protein)               :: 5,320
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 237
            CDSs (without protein)            :: 237
            Pseudo Genes (ambiguous residues) :: 0 of 237
            Pseudo Genes (frameshifted)       :: 102 of 237
            Pseudo Genes (incomplete)         :: 130 of 237
            Pseudo Genes (internal stop)      :: 52 of 237
            Pseudo Genes (multiple problems)  :: 41 of 237
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4407
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267800-rid25106413.denovo.304"
                     /serotype="O 26:H11"
                     /isolate="2201S31241"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-01"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            complement(22..219)
                     /locus_tag="ACHOSC_RS21675"
                     /old_locus_tag="pgaptmp_004335"
     CDS             complement(22..219)
                     /locus_tag="ACHOSC_RS21675"
                     /old_locus_tag="pgaptmp_004335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TrmB family transcriptional regulator"
                     /protein_id="HGU4550137.1"
                     /translation="MLKQQDMTETARVVFNELSVTEPATVGEVAQNTYLSRERCQLIL
                     TQLVMAGLADYQCGCYRRLQS"
     gene            complement(461..991)
                     /locus_tag="ACHOSC_RS21680"
                     /old_locus_tag="pgaptmp_004336"
     CDS             complement(461..991)
                     /locus_tag="ACHOSC_RS21680"
                     /old_locus_tag="pgaptmp_004336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1133 family protein"
                     /protein_id="HGU4550138.1"
                     /translation="MIYPEITGKSGEHLRLNTLEAVWIQGKLRMWGRWSYIGGGKSGN
                     MFNRLLVSKKLTKTAVNEVLRSMKKSGLEKPELEAFFRDMTRGKQKSWLSHCTDTEAL
                     IIDRVISEVLGEYPGLINILRQRYEGRGMSKRKMAECLNRTHPEWCFSTCEKRIAGWL
                     AVAEHMLYVPMHDSFR"
     gene            complement(1000..1359)
                     /locus_tag="ACHOSC_RS21685"
                     /old_locus_tag="pgaptmp_004337"
     CDS             complement(1000..1359)
                     /locus_tag="ACHOSC_RS21685"
                     /old_locus_tag="pgaptmp_004337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RusA family crossover junction
                     endodeoxyribonuclease"
                     /protein_id="HGU4550139.1"
                     /translation="MLIDLVLPYPPTVNTYWRRRGSTYFISEEGKRYRRAVALIVRQQ
                     RLKLSLSGRLAIKVIAEPPDKRRRDLDNILKAPLDALTHAEVLIDDEQFDEINIVRGQ
                     PVSGGRLGVKIYKIESE"
     gene            complement(1372..2418)
                     /locus_tag="ACHOSC_RS21690"
                     /old_locus_tag="pgaptmp_004338"
     CDS             complement(1372..2418)
                     /locus_tag="ACHOSC_RS21690"
                     /old_locus_tag="pgaptmp_004338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF968 domain-containing protein"
                     /protein_id="HGU4550140.1"
                     /translation="MRVLLRPVLVPELGLVIVKPGRESMPVFHNTRVLVEPEPKSMRN
                     LPSGVVPAVRQPLVEDKTLLPFFSNARVIRAAGGAGALSDWLLRHIKSCQWPHGDYHH
                     SETVIHRYGTGAMVLCWHCDNQLRDQTSESLGQLAQQNLTAWMIDVIRHAISGAQERE
                     LSLAELSWWAVRNQVADALPEAVLRRSLGLRAEKIRSMYRESDIVPGEQTATSILKQR
                     TKNLAPLPHAHQQKPPQEKTVVSIAVDPESPESFMKRPKRRRWVNEKYTHWVKTQPCA
                     CCGKPADDPHHLIGHGQGGMGTKSHDIFTLPLCREHHNELHADPLAFEEKHGSQVDLI
                     FRFLDHAFATGVLG"
     gene            complement(2420..2692)
                     /locus_tag="ACHOSC_RS21695"
                     /old_locus_tag="pgaptmp_004339"
     CDS             complement(2420..2692)
                     /locus_tag="ACHOSC_RS21695"
                     /old_locus_tag="pgaptmp_004339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4550141.1"
                     /translation="MAHNTKHHNSDIAPASRQGRCSHTFKYDWYQHDPCTEEQAEWLI
                     HNYRRRGYEFKKALSLDYRHWIIYVRLLYSERPPRPSRTFQQRIWR"
     gene            2828..3085
                     /locus_tag="ACHOSC_RS21700"
                     /old_locus_tag="pgaptmp_004340"
     CDS             2828..3085
                     /locus_tag="ACHOSC_RS21700"
                     /old_locus_tag="pgaptmp_004340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001297790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0051983 - regulation of chromosome
                     segregation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system ParD family
                     antitoxin"
                     /protein_id="HGU4550142.1"
                     /translation="MRTTQQFSITLTNEMADMVRARVASGAYASESEVIREGLRALNE
                     RDKAIEAWLTHSAAPSLDSIRENPNNGRSISQVRAAIRSGK"
     gene            3091..3390
                     /locus_tag="ACHOSC_RS21705"
                     /old_locus_tag="pgaptmp_004341"
     CDS             3091..3390
                     /locus_tag="ACHOSC_RS21705"
                     /old_locus_tag="pgaptmp_004341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000220602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008657 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) inhibitor activity
                     [Evidence IEA];
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA];
                     GO_process: GO:0030541 - plasmid partitioning [Evidence
                     IEA];
                     GO_process: GO:0051290 - protein heterotetramerization
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HGU4550143.1"
                     /translation="MTYEVIITPEAEQQIINLHRYITEKAGNVIADNYANALLDYLDG
                     FSTFPHRGNKRDDIRQGMRVTHFRHRTIIAFAVDSRKVFIVGIYHGGQSYETDFL"
     gene            complement(3595..3939)
                     /locus_tag="ACHOSC_RS21710"
                     /old_locus_tag="pgaptmp_004342"
     CDS             complement(3595..3939)
                     /locus_tag="ACHOSC_RS21710"
                     /old_locus_tag="pgaptmp_004342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000206826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4550144.1"
                     /translation="MTTFTREQLIAHAEETIETQRLCIPGTIDHDIIRTYKMDIAVLE
                     IALVSLAAEPAGKLHEYKPVGYQRLVDELTMLVKQLTWQLRKAKPDCKLPDKAMSYLE
                     RNGLISVEDILR"
     gene            complement(3936..4301)
                     /locus_tag="ACHOSC_RS21715"
                     /old_locus_tag="pgaptmp_004343"
     CDS             complement(3936..4301)
                     /locus_tag="ACHOSC_RS21715"
                     /old_locus_tag="pgaptmp_004343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease signature motif containing
                     protein"
                     /protein_id="HGU4550145.1"
                     /translation="MALTKKQRAELRMKFGGRCAYCGCELGEKWHADHVKPVIRFDGN
                     MLHQERDDISNMVPACHPCNLHKHCSSLEDYRRIISDGRREFLASGKGKALVRMGLVE
                     MKSDPVVFWFEKYQEGATA"
     gene            complement(4303..>4407)
                     /locus_tag="ACHOSC_RS21720"
                     /old_locus_tag="pgaptmp_004344"
     CDS             complement(4303..>4407)
                     /locus_tag="ACHOSC_RS21720"
                     /old_locus_tag="pgaptmp_004344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001585821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4014 family protein"
                     /protein_id="HGU4550146.1"
                     /translation="FIVKIIEPVIELYNDVVWASFSTLHNKINPYKES"
BASE COUNT         1058 a         1196 c          984 g         1169 t
ORIGIN      
        1 ttcaccacaa ataaaaagcc ttcaggactg aaggcgtctg taacaaccgc actgatagtc
       61 tgccagaccc gccataacaa gctgggtcag tattaactgg cagcgttcgc gtgaaaggta
      121 agtattctgc gcaacctccc cgactgtcgc cggttcggta acgcttaatt cattaaacac
      181 cactctggcg gtttcggtca tatcctgctg tttcagcatg tctttttccc tttttcggtt
      241 aacgtgacac accaataact cttgtcgaaa aagccagcaa gctgaaagaa cggtattaat
      301 aaccaccagc gaatttattg cgctgctgta tattacggac acaaaaaaac caccttccgg
      361 tggcttcctt gtgcgaaaaa acttgcattt cgcctcgcga tacagctttg cgaagcttac
      421 aggaattcaa gctgtttctg cgtaaaaaag caagcttttt ttatcgaaat gaatcgtgca
      481 taggtacata aagcatgtgt tcagccacgg ctaaccaacc tgcaatacgt ttctcacatg
      541 tgctgaaaca ccattccggg tgagtacgat ttaaacattc tgccattttt ctcttactca
      601 ttccccgtcc ttcgtacctt tgccggagaa tattgattag cccgggatat tccccaagca
      661 cctcactgat aacgcgatca ataatcaacg cctctgtgtc tgtacaatgt gacaaccagc
      721 tcttctgctt ccctctggtc atatcccgaa aaaatgcctc aagttccggt ttttccagcc
      781 cggatttctt catgctgcgt aaaacctcat taactgctgt tttcgtcagc tttttcgaaa
      841 ccagtaaccg gttaaacata tttccggatt tacccccacc gatatacgac caccgccccc
      901 acatccgtaa tttcccctgg atccagactg cttccagcgt gttcaggcgt aaatgttcgc
      961 cgcttttgcc tgtaatttcc ggatatatca tatttacgct cactcactct caattttgta
     1021 aatcttcaca cccagccgtc caccagatac tggctgaccg cgcacaatat tgatttcatc
     1081 aaactgctcg tcatcaatga gcacttccgc atgcgtcagc gcatccagcg gtgctttcag
     1141 aatgttgtcc aggtcacggc ggcgcttatc cggtggctct gcaatcacct ttatcgccag
     1201 ccttccggac aggcttaatt tcagccgctg ctggcgaaca ataagcgcca cagcccggcg
     1261 ataacgcttt ccctcctccg agataaaata tgtgctgcca cggcgtcgcc agtaagtgtt
     1321 caccgtcggc gggtaaggta aaaccaaatc tatgagcatc agtcacctct tttacccaag
     1381 cacgccagtt gcaaaggcgt gatcaagaaa acgaaaaatt aaatcaacct gagaaccatg
     1441 cttttcttcg aacgccagcg gatccgcatg aagctcgttg tgatgctccc gacacagcgg
     1501 tagcgtgaaa atatcgtgag attttgtccc cattcccccc tgaccatgac caatcaggtg
     1561 atgcggatcg tcagcaggct taccacaaca cgcacacggc tgtgtcttca cccagtgcgt
     1621 gtatttctcg ttaacccagc ggcgacgttt aggtcgtttc atgaaagatt ccggagactc
     1681 aggatcaacg gcaatgctga ccaccgtctt ttcctgtggc ggtttttgct ggtgagcgtg
     1741 aggcagcggc gcaagatttt ttgtgcgctg cttcagtatg ctggtggcgg tctgctctcc
     1801 cggtacgatg tcgctttcac ggtacattga gcggattttt tccgcacgca accccagcga
     1861 acgacgtaat accgcttccg gtagcgcgtc cgccacctga ttgcggaccg cccaccagga
     1921 taattcagcc agagataatt cccgttcctg tgcaccactt attgcgtgac gtatgacgtc
     1981 aatcatccag gctgtcagat tttgttgagc aagctgcccg agtgattcgg atgtctggtc
     2041 gcgcagctgg ttgtcgcagt gccagcacaa caccattgcg ccggtaccat aacggtgaat
     2101 gacggtttcg ctgtgatgat aatcgccgtg tggccactgg caggatttaa tatggcgcaa
     2161 cagccagtca gacaatgcac cagcaccacc agcagcacga atcacccgtg cgttactgaa
     2221 aaacggcagc aatgttttgt cttccactag cggctggcga acggcaggaa caaccccgga
     2281 cggcagatta cgcatgcttt tcggttccgg ctccaccagt acccgggtat tgtggaatac
     2341 cggcatggat tcacgacccg gcttaacgat caccagcccg agttccggta ccagaacagg
     2401 tcgaagtaat acccgcacgt tacctccaga tgcgttgctg gaatgtgcgg gacggacgcg
     2461 gcgggcgttc ggaataaagg agcctgacgt agattatcca gtgacggtag tcgaggctaa
     2521 gggctttctt aaactcatac ccacgtctgc ggtagttatg aatcagccat tcggcctgtt
     2581 cttcagtgca gggatcgtgc tgataccagt catatttgaa tgtgtgagaa caccgccctt
     2641 gcctgctggc aggggcgata tcagaattgt gatgtttggt attgtgcgcc atcggttttc
     2701 tctgctggcg cagcaggtgc cagttgttca agctggcgtg cggcaatatt gtctctgatt
     2761 tctgttgtcg tcaacaggca acgtgctatc atcgaatggt gttctatcct actccgtgag
     2821 gtttaccatg cgtacaaccc aacaattcag cattacatta actaacgaaa tggctgacat
     2881 ggtgcgcgcc cgtgtggctt ccggtgccta tgcttcagaa agcgaggtca ttcgtgaagg
     2941 gcttcgcgca ctgaatgagc gcgataaagc aatcgaagcg tggttaacgc attcagccgc
     3001 cccctctctt gattctatcc gcgaaaatcc aaacaacgga cgctccattt cacaggttcg
     3061 cgccgcgatt cgatccggga agtaatctgc atgacatatg aagtcatcat tactcctgag
     3121 gccgaacaac aaataatcaa cctgcacagg tatataacgg agaaagcagg gaacgtcatt
     3181 gctgacaatt atgccaatgc gcttcttgat tatcttgatg ggttttctac attcccgcat
     3241 cggggcaata aacgcgatga tattcgccag gggatgcggg taactcattt ccgccacaga
     3301 acgattattg cttttgccgt tgatagcaga aaagtcttta ttgtcggtat ctatcatggt
     3361 gggcaaagtt atgaaaccga tttcttataa acttttaccc acatcattcc ggtgttagaa
     3421 ttaaccgtcc gccccctctc ttactggcgg attcgtaggc tatataaatc aaagatcccg
     3481 gctcatgttt atgtcgggat cttttttcgg cgatttatcc ccagcggcaa atcgaataca
     3541 ccaccagcgc caccgccatc gcaattccta ccgttgtgaa tgcttcaggc caggtcatcg
     3601 taaaatatcc tccacgctta tcagtccgtt ccgctccaga taactcatcg ccttatccgg
     3661 taatttgcag tctggcttcg ctttcctcag ttgccaggtt aactgcttta ccagcatggt
     3721 taactcatcg accagacgct gatatcccac tggtttgtat tcatgcaatt taccggctgg
     3781 ctctgctgcc agcgatacca gtgcgatttc cagaacagca atatccatct tatatgtgcg
     3841 gatgatgtca tggtcgattg tgcccggtat gcacagtctc tgtgtttcaa tagtctcctc
     3901 tgcgtgagct attaactgct ctctggtaaa agtcgtcatg ccgtagcccc ttcttgatat
     3961 ttttcaaacc agaacacaac tgggtcagat ttcatttcaa ccaatcccat acgaaccagc
     4021 gctttgcctt tcccggacgc aaggaattca cgacgaccat cactgataat tcgccgataa
     4081 tcttccagac tactgcaatg cttgtgcaga ttgcatgggt ggcatgccgg aaccatgttg
     4141 gatatatcgt cacgttcctg gtgaagcata tttccatcaa aacgaatgac cggttttaca
     4201 tggtctgcat gccacttttc gccaagttcg cagccgcaat aagcacagcg accgccgaac
     4261 ttcatgcgca gctctgcacg ttgttttttc gtcagtgcca tatcagcttt ccttatacgg
     4321 attaatttta ttgtgcagtg tgctgaacga cgcccacacc acgtcgttat acaattcaat
     4381 aactggctca attattttca cgataaa
//
LOCUS       DAZKHF020000303         4393 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2201S31241
            SAMN43267800-rid25106413.denovo.305, whole genome shotgun sequence.
ACCESSION   DAZKHF020000303 DAZKHF020000000
VERSION     DAZKHF020000303.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267800
            Sequence Read Archive: SRR30307263
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4393)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4393)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 4393)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350581.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350581.2
            Genome Coverage       :: 151x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:21:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,652
            CDSs (total)                      :: 5,557
            Genes (coding)                    :: 5,320
            CDSs (with protein)               :: 5,320
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 237
            CDSs (without protein)            :: 237
            Pseudo Genes (ambiguous residues) :: 0 of 237
            Pseudo Genes (frameshifted)       :: 102 of 237
            Pseudo Genes (incomplete)         :: 130 of 237
            Pseudo Genes (internal stop)      :: 52 of 237
            Pseudo Genes (multiple problems)  :: 41 of 237
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4393
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267800-rid25106413.denovo.305"
                     /serotype="O 26:H11"
                     /isolate="2201S31241"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-01"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            <1..75
                     /locus_tag="ACHOSC_RS21725"
                     /old_locus_tag="pgaptmp_004345"
     CDS             <1..75
                     /locus_tag="ACHOSC_RS21725"
                     /old_locus_tag="pgaptmp_004345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309203.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0060703 - deoxyribonuclease inhibitor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="host-nuclease inhibitor Gam family protein"
                     /protein_id="HGU4550147.1"
                     /translation="RMAEHIRYMVETIAHHQVDIDSEV"
     gene            81..866
                     /gene="bet"
                     /locus_tag="ACHOSC_RS21730"
                     /old_locus_tag="pgaptmp_004346"
     CDS             81..866
                     /gene="bet"
                     /locus_tag="ACHOSC_RS21730"
                     /old_locus_tag="pgaptmp_004346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage recombination protein Bet"
                     /protein_id="HGU4550148.1"
                     /translation="MSTALATLAGKLAERVGMDSVDPQELITTLRQTAFKGDASDAQF
                     IALLIVANQYGLNPWTKEIYAFPDKQNGIVPVVGVDGWSRIINENQQFDGMDFEQDNE
                     SCTCRIYRKDRNHPICVTEWMDECRREPFKTREGREITGPWQSHPKRMLRHKAMIQCA
                     RLAFGFAGIYDKDEAERIVENTAYTAERQPERDITPVNDETMQEINTLLIALDKTWDD
                     DLLPLCSQIFRRDIRASSELTQAEAVKALGFLKQKATEQKVAA"
     gene            863..1540
                     /locus_tag="ACHOSC_RS21735"
                     /old_locus_tag="pgaptmp_004347"
     CDS             863..1540
                     /locus_tag="ACHOSC_RS21735"
                     /old_locus_tag="pgaptmp_004347"
                     /EC_number="3.1.11.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004527 - exonuclease activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lambda exonuclease family protein"
                     /protein_id="HGU4550149.1"
                     /translation="MTPDIILQRTGIDVRAVEQGDDAWHKLRLSVITASEIHNVIAKP
                     RSGKKWPDMKMSYFHTLLAEVCTGVAPEVNAKALAWGKQYENDARTLFEFTSGVNVTE
                     SPIIYRDESMRTACSPDGLCSDGNGLELKCPFTSRDFMKFRLGGFEAIKSAYMAQVQY
                     SMWVTRKDAWYFANYDPRMKREGLHYVVIERNEKYMASFDEMVPEFIEKMDEALAEIG
                     FVYGEQW"
     gene            1540..1722
                     /locus_tag="ACHOSC_RS21740"
                     /old_locus_tag="pgaptmp_004348"
     CDS             1540..1722
                     /locus_tag="ACHOSC_RS21740"
                     /old_locus_tag="pgaptmp_004348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311030.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1317 domain-containing protein"
                     /protein_id="HGU4550150.1"
                     /translation="MKHPHDNIRVGTITFVYSVTKRGWVFPGLSVIRNPLKAQRLAEE
                     INNKRGAVCTKHLLLN"
     gene            1695..1886
                     /locus_tag="ACHOSC_RS21745"
                     /old_locus_tag="pgaptmp_004349"
     CDS             1695..1886
                     /locus_tag="ACHOSC_RS21745"
                     /old_locus_tag="pgaptmp_004349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1382 family protein"
                     /protein_id="HGU4550151.1"
                     /translation="MHKASPVELRTSIGMAHSLAQIGVRFVPIPVETDEEFHTLATSL
                     SQKLEMMAAKAEANERDPA"
     gene            1963..2178
                     /locus_tag="ACHOSC_RS21750"
                     /old_locus_tag="pgaptmp_004350"
     CDS             1963..2178
                     /locus_tag="ACHOSC_RS21750"
                     /old_locus_tag="pgaptmp_004350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000151206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapA"
                     /protein_id="HGU4550152.1"
                     /translation="MTPQQENALRSIARQANSEIKKARQQFPDKNVDDICRSVLKKHR
                     ETVTLMGFTPTHLSLAIGMLNGVFKER"
     gene            2277..2498
                     /locus_tag="ACHOSC_RS21755"
                     /old_locus_tag="pgaptmp_004351"
     CDS             2277..2498
                     /locus_tag="ACHOSC_RS21755"
                     /old_locus_tag="pgaptmp_004351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TraR/DksA family transcriptional regulator"
                     /protein_id="HGU4550153.1"
                     /translation="MADIIDSASEIEELQRNTAIKMHRLNHQAISATHCCECGDPIDE
                     LRRLAVQGCRTCASCQEDLELISKQRGSK"
     gene            2495..2800
                     /locus_tag="ACHOSC_RS21760"
                     /old_locus_tag="pgaptmp_004352"
                     /pseudo
     CDS             2495..2800
                     /locus_tag="ACHOSC_RS21760"
                     /old_locus_tag="pgaptmp_004352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311034.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ead/Ea22-like family protein"
     gene            3097..3447
                     /locus_tag="ACHOSC_RS21765"
                     /old_locus_tag="pgaptmp_004353"
     CDS             3097..3447
                     /locus_tag="ACHOSC_RS21765"
                     /old_locus_tag="pgaptmp_004353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001345188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF551 domain-containing protein"
                     /protein_id="HGU4550154.1"
                     /translation="MINRTKLEHILEYARQQRRFGQLCKILPGDMVEIVEIAMRKTGN
                     SPVSPGGWISCSERMPAQDDWILIYSKHGEYMAGQVQEEYVELSDGTLSWLGNALYWM
                     LLPEPPQGVNDELA"
     gene            3522..3764
                     /locus_tag="ACHOSC_RS21770"
                     /old_locus_tag="pgaptmp_004354"
     CDS             3522..3764
                     /locus_tag="ACHOSC_RS21770"
                     /old_locus_tag="pgaptmp_004354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000457709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4222 domain-containing protein"
                     /protein_id="HGU4550155.1"
                     /translation="MFALIQRGQIYTDSAGYPIKILRCINNTVLYRRMDGRTQSVKIN
                     DFNELFERIDHQEYRQILAETEQENHLKKLRAMQRR"
     gene            3883..4227
                     /locus_tag="ACHOSC_RS21775"
                     /old_locus_tag="pgaptmp_004355"
     CDS             3883..4227
                     /locus_tag="ACHOSC_RS21775"
                     /old_locus_tag="pgaptmp_004355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016248831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4550156.1"
                     /translation="MSDLAMKVLKWQSTGDVGISSATLASIACGLKKNIYGHHFGAPH
                     DAADFRRCVALVEQIPEIRDSFDKVAKRVPAFKGILNEWDSLVALLKSEMKIHGNKAP
                     ETYRRISELRKD"
     gene            4333..>4393
                     /locus_tag="ACHOSC_RS21780"
                     /old_locus_tag="pgaptmp_004356"
     CDS             4333..>4393
                     /locus_tag="ACHOSC_RS21780"
                     /old_locus_tag="pgaptmp_004356"
                     /inference="COORDINATES: protein motif:HMM:NF019442.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excisionase"
                     /protein_id="HGU4550157.1"
                     /translation="MYLTLQEWNARQRRPRSLET"
BASE COUNT         1251 a         1005 c         1144 g          993 t
ORIGIN      
        1 cgcatggcag aacacatccg gtacatggtt gaaaccattg ctcaccacca ggttgatatt
       61 gattcagagg tataaaacgg atgagtacag cactcgcaac gctggctggg aagctggctg
      121 aacgtgtcgg catggattct gtcgacccac aggaactgat caccactctt cgccagacgg
      181 catttaaagg tgatgccagc gatgcgcagt tcatcgcatt gctgatcgtc gccaaccagt
      241 acggccttaa tccgtggacg aaagaaattt acgccttccc tgataagcag aacggcatcg
      301 ttccggtggt gggcgttgat ggctggtccc gcatcatcaa cgaaaaccag cagtttgatg
      361 gcatggactt tgagcaggac aatgaatcct gcacatgccg gatttaccgc aaggaccgta
      421 atcatccgat ctgcgttacc gagtggatgg atgaatgccg ccgcgaacca ttcaaaaccc
      481 gcgaaggcag agaaatcacg gggccgtggc agtcgcatcc caaacggatg ttacgtcata
      541 aagccatgat tcagtgtgcc cgtctcgcct tcggatttgc tggtatctat gacaaggatg
      601 aagccgagcg cattgtcgaa aataccgcat acactgcaga acgtcagccg gaacgcgaca
      661 tcactccggt taacgatgaa accatgcagg aaattaacac tctgctgatc gccctggata
      721 aaacatggga tgacgactta ttgccgctct gttcccagat atttcgccgc gacattcgtg
      781 catcgtcaga actgacacag gccgaagcag taaaagctct tggattcctg aaacaaaaag
      841 ccactgaaca gaaggtggca gcatgacacc ggacattatc ctgcagcgta ctgggatcga
      901 cgtgagagct gtcgaacagg gagatgatgc gtggcacaaa ttacggctca gcgtcatcac
      961 agcttcagaa attcacaacg taatagcaaa accccgatca ggaaagaagt ggcctgacat
     1021 gaaaatgtcc tacttccaca ccctgctggc tgaggtttgc accggtgtgg ctccggaagt
     1081 taacgctaag gctctggcct ggggaaaaca gtacgagaac gacgccagaa ccctctttga
     1141 gttcacttcc ggcgtgaatg ttactgaatc cccgatcatc tatcgcgacg aaagtatgcg
     1201 caccgcctgc tctcccgatg gtttatgcag tgacggcaac ggccttgagc tgaaatgccc
     1261 gtttacctcc cgggatttca tgaagttccg gctcggtggt ttcgaggcca taaaatcggc
     1321 ttacatggcc caggtgcagt acagcatgtg ggtgacacga aaagatgcct ggtactttgc
     1381 caactatgac ccgcgcatga agcgtgaagg cctgcattat gtcgtgattg agcggaatga
     1441 aaagtacatg gcgagttttg acgagatggt gccggagttc atcgaaaaaa tggacgaggc
     1501 actggctgaa attggttttg tatatgggga gcaatggtaa tgaagcatcc tcacgataat
     1561 atccgggtag gcacgatcac tttcgtctac tccgttacaa agcgaggctg ggtatttccc
     1621 ggcctttctg ttatccgaaa tccactgaaa gcacagcggc tggctgaaga gataaataat
     1681 aaacgagggg ctgtatgcac aaagcatctc ctgttgaatt aagaacgagt atcgggatgg
     1741 cacatagcct cgctcaaatt ggagtcaggt ttgtgccaat accagtagaa acagacgaag
     1801 aatttcatac gttagccaca tccctttcac aaaagctgga aatgatggcg gcgaaagcag
     1861 aagcaaacga gagagacccg gcatgacaac aacagaatgc atttttctgg cagcaggctt
     1921 catattctgt gtgcttatgc ttgccgacat gggacttgtt caatgacacc tcagcaggaa
     1981 aacgcccttc gcagcattgc ccgtcaggct aattctgaaa tcaaaaaagc cagacagcag
     2041 tttccggata aaaacgtcga tgacatttgc cgtagcgtac tgaagaagca ccgcgaaacg
     2101 gtaacgctaa tgggattcac accgactcat ttaagtctgg cgatcggcat gttaaacggc
     2161 gtctttaagg agcgatgaac atgaaaagca aaatcatcag ggagctacag gctccttttt
     2221 tattgttcgc attcaccctc aagcgtatta accaacaatt cagggattaa tggaagatgg
     2281 cagacatcat tgattcagca tcagaaattg aagaattaca gcgcaacaca gcaataaaaa
     2341 tgcaccgcct gaaccaccag gctatatctg ccactcattg ttgtgagtgt ggcgatccga
     2401 tagatgaact aagacgtctg gccgttcagg gttgtcggac ttgtgcaagt tgccaagagg
     2461 atctggagct tatcagtaaa cagagaggtt cgaagtgagc gaaattaact ctcaggcact
     2521 gcgtgaagcg gcagagcagg caatgcatga cgactgggga tttgatgcgg accttttcca
     2581 tgagctggta acaccatcga ttgtgctggc actgctggat gaacgggaaa gaaaccagca
     2641 atacatcaaa cgccgcgacc aggagaacga ggaaattgcg ctaacggtag ggaagctgcg
     2701 tgttgagctt gaggaagcaa aatcaaaact caacgagcag cgtgagtatt acgaaggtgt
     2761 tatctcggat gggagtaaac gtattgctga actggagaaa agcgaagagc aactcattaa
     2821 cgagcgtgac catgctgagt ctgctttagc tgatatgtac ttcgcaacaa ccggggatag
     2881 gcctgagtgg agtaactgtt tcagtttttc agatgctgtc gatgccgtag ttgacagaat
     2941 tgctgattta gaagccaaac agccatcgcc agtagtaccg gaagaaaaac caatgcctaa
     3001 ccctcttagc atgtacacag ttgatgctgt tgcagctatt gcagaggtga gaggctggaa
     3061 cgcctgccgt gcagccatgc ttaagggaga taaatcatga ttaatcgaac caaactggag
     3121 cacatcctcg agtatgccag gcagcagagg cgctttggcc agctttgtaa aattctgcca
     3181 ggagatatgg ttgaaatcgt ggagattgcc atgcgtaaga ctggcaactc tccggtaagt
     3241 ccgggtggtt ggataagctg tagtgagcga atgcccgctc aagatgattg gattttaatt
     3301 tattcaaagc acggtgagta tatggcagga caggtacaag aggaatacgt ggagttgagc
     3361 gacggcactt tatcgtggtt agggaacgcc ttgtactgga tgctgctacc agaaccgccg
     3421 cagggagtga atgatgaatt ggcctgaagc attcaccgct gtaggagttg caatcgcggt
     3481 ggcatttatt ctgtattcgc ttttccgctg gggataaagg aatgttcgct ctgattcaac
     3541 gtggtcagat atacaccgat agcgccggct acccgataaa aattcttcgc tgcataaaca
     3601 acactgtgtt gtacagaaga atggatgggc gaacacagtc ggtaaaaata aacgatttta
     3661 atgaactgtt tgaacggatc gatcaccagg aataccgaca aattctggct gaaacagagc
     3721 aggagaacca tctgaaaaaa ttacgcgcca tgcaaaggag ataaaccggt aaaggtgttc
     3781 gcgataaagg tgaatatcgg caatgaataa caatcctcgc actcgcgggg atttctttta
     3841 tctgaactcg ctacggcggg ttttgtttta tggagacaag aaatgtcaga tttggctatg
     3901 aaggttttga aatggcaatc gactggcgat gttggcatca gtagcgcaac tcttgcctca
     3961 atcgcatgtg gactgaaaaa gaatatctat ggtcatcact tcggcgctcc acatgacgca
     4021 gcagacttcc ggcgatgcgt tgcacttgtt gagcagattc cagaaatcag agattcattc
     4081 gacaaggttg caaagcgcgt tccggcattc aaaggcatcc tcaacgaatg ggattccctc
     4141 gtcgctctgt tgaagtctga aatgaagata cacggaaaca aagcaccaga gacttacaga
     4201 agaattagcg agctacgcaa ggactaacca cagcctcaca ctcaatgagg cctgttcatt
     4261 tctcaagata tccagaccta ccattgccgc atcaatgcgg cttttcttgc gtgtaattgc
     4321 ggagactttg cgatgtactt gacacttcag gagtggaatg cacgccagcg acgcccaaga
     4381 agccttgaaa cag
//
LOCUS       DAZKHF020000304         4243 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2201S31241
            SAMN43267800-rid25106413.denovo.306, whole genome shotgun sequence.
ACCESSION   DAZKHF020000304 DAZKHF020000000
VERSION     DAZKHF020000304.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267800
            Sequence Read Archive: SRR30307263
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4243)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4243)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 4243)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350581.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350581.2
            Genome Coverage       :: 151x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:21:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,652
            CDSs (total)                      :: 5,557
            Genes (coding)                    :: 5,320
            CDSs (with protein)               :: 5,320
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 237
            CDSs (without protein)            :: 237
            Pseudo Genes (ambiguous residues) :: 0 of 237
            Pseudo Genes (frameshifted)       :: 102 of 237
            Pseudo Genes (incomplete)         :: 130 of 237
            Pseudo Genes (internal stop)      :: 52 of 237
            Pseudo Genes (multiple problems)  :: 41 of 237
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4243
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267800-rid25106413.denovo.306"
                     /serotype="O 26:H11"
                     /isolate="2201S31241"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-01"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            17..1075
                     /gene="tsuA"
                     /locus_tag="ACHOSC_RS21785"
                     /old_locus_tag="pgaptmp_004357"
     CDS             17..1075
                     /gene="tsuA"
                     /locus_tag="ACHOSC_RS21785"
                     /old_locus_tag="pgaptmp_004357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiosulfate utilization transporter TsuA/YeeE"
                     /protein_id="HGU4550158.1"
                     /translation="MFSMILSGLICGALLGFVMQRGRFCLTGGFRDMYIAKNNRMFYA
                     LLIAISVQSVGVFALIQAGLLTYEAGAFPWLGTVIGGYLFGLGIVLAGGCATGTWYRA
                     GEGLIGSWIALFTYMVMSAVMRSPHASGLNQTLQHYTTEHNSIADTFNLSVWPLVAVL
                     LVITLWVVMKELKKPKLKVATLPPRRTGIAHILFEKRWHPFVTAVLIGLIALLAWPLS
                     EATGRMFGLGITSPTANILQFLVAGDVKYINWGVFLVLGIFVGSFIAAKASREFRVRA
                     ADAQTTLRSGLGGVLMGFGASIAGGCSIGNGLVMTAMMTWQGWIGLVFMILGVWTASW
                     LVYVRPQRKARLATAAAN"
     gene            1089..1316
                     /gene="tsuB"
                     /locus_tag="ACHOSC_RS21790"
                     /old_locus_tag="pgaptmp_004358"
     CDS             1089..1316
                     /gene="tsuB"
                     /locus_tag="ACHOSC_RS21790"
                     /old_locus_tag="pgaptmp_004358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiosulfate utilization sulfurtransferase
                     TsuB/YeeD"
                     /protein_id="HGU4550159.1"
                     /translation="MAIKKLDVVTQVCPFPLIEAKAALAEMASGDELVIEFDCTQATE
                     AIPQWAAEEGHAITDYQQIGDAAWSITVQKA"
     gene            complement(1359..2786)
                     /gene="sbcB"
                     /locus_tag="ACHOSC_RS21795"
                     /old_locus_tag="pgaptmp_004359"
     CDS             complement(1359..2786)
                     /gene="sbcB"
                     /locus_tag="ACHOSC_RS21795"
                     /old_locus_tag="pgaptmp_004359"
                     /EC_number="3.1.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000175 - 3'-5'-RNA exonuclease activity
                     [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0008310 - single-stranded DNA 3'-5' DNA
                     exonuclease activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease I"
                     /protein_id="HGU4550160.1"
                     /translation="MMNDGKQQSTFLFHDYETFGTHPALDRPAQFAAIRTDDEFNVIG
                     EPEVFYCKPADDYLPQPGAVLITGITPQETRAKGENEAAFAARIHSLFTVPKTCILGY
                     NNVRFDDEVTRNVFYRNFYDPYAWSWQHDNSRWDLLDVMRACYALRPEGINWPENDDG
                     LPSFRLEHLTKANGIEHSNAHDAMADVYATIAMAKLVKTRQPRLFDYLFTHRNKHKLM
                     ALIDVPQMKPLVHVSGMFGAWRGNTSWVAPLAWHPENRNAVIMVDLAGDISPLLELDS
                     DTLRERLYTAKADLGDNAAVPVKLVHINKCPVLAQANTLRPEDADRLGINRQHCLDNL
                     KILRENPQVREKVVAIFVEAEPFTPSDNVDAQLYNGFFSDADRAAMKIVLETEPRNLP
                     ALDITFVDKRIEKLLFNYRARNFPGTLDYAEQQRWLEHRRQVFTPEFLQGYAEEIQML
                     AQQYADDKEKVALLKALWQYAEEIV"
     gene            2995..4161
                     /gene="dacD"
                     /locus_tag="ACHOSC_RS21800"
                     /old_locus_tag="pgaptmp_004360"
     CDS             2995..4161
                     /gene="dacD"
                     /locus_tag="ACHOSC_RS21800"
                     /old_locus_tag="pgaptmp_004360"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416514.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009002 - serine-type D-Ala-D-Ala
                     carboxypeptidase activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine-type D-Ala-D-Ala carboxypeptidase DacD"
                     /protein_id="HGU4550161.1"
                     /translation="MKRRLIIAASLFIFNLSSGFAAENIPFSPQPPEIHAGSWVLMDY
                     TTGQILTAGNEHQQRNPASLTKLMTGYVVDRTIDSHRITPDDIVTVGRDAWAKDNPVF
                     VGSSLMFLKEGDRVSVRDLSRGLIVDSGNDACVALADYIAGGQRQFVEMMNNYAEKLH
                     LKDTHFETVHGLDAPGQHSSAYDLAVLSRAIIHDEPEFYHMYSEKSLTWNGITQQNRN
                     GLLWDKTMNVDGLKTGHTSGAGFNLIASAVDGQRRLIAVVMGADSAKGREEEARKLLR
                     WGQQNFTTVQILHRGKKVGTERIWYGDKENIDLGTEQEFWMVLPKAEIPHIKAKYTLD
                     GKELTAPISAHQRVGEIELYDRDKQVAHWPLVTLESVGEGSMFSRLSDYFHHKA"
BASE COUNT         1011 a          972 c         1116 g         1144 t
ORIGIN      
        1 tctggttaat taatttatgt tttcaatgat attaagcggg ctaatttgtg gtgctctgct
       61 gggatttgtc atgcagcgtg ggcgtttttg cctgacaggt ggttttcgcg atatgtatat
      121 cgcgaaaaat aatcgcatgt tttacgcctt gctgattgca atttcggtac aaagcgtggg
      181 ggtttttgcg ttaattcagg cgggcctact gacttacgaa gccggggcgt tcccgtggct
      241 tggtactgtt ataggtgggt atcttttcgg gctgggaatt gttctggcgg gtggatgtgc
      301 caccgggacc tggtatcgcg cgggtgaggg attgatcggc agttggatcg cgcttttcac
      361 ttatatggtg atgagtgcgg tgatgcgttc tccacacgcc agtggtttaa atcaaacctt
      421 gcagcactac actactgaac ataactctat tgctgatact tttaatttgt ctgtgtggcc
      481 gttggttgcc gttttgctgg tgataacgct ctgggtggtg atgaaagagt tgaagaagcc
      541 aaaactcaaa gtcgcgacct taccgccgcg ccgaaccggg atcgctcata ttctgtttga
      601 aaaacgctgg catccctttg tcactgctgt actcatcggt ttgattgcgc ttttagcttg
      661 gcccctgagt gaagcaaccg gacgcatgtt tgggttagga atcacttccc caacggccaa
      721 tattctgcaa tttctggtcg cgggtgacgt gaaatacatt aactggggcg ttttcctggt
      781 gttagggatc ttcgtggggt catttattgc agccaaagcg agccgtgagt tccgcgttcg
      841 cgctgctgat gcacaaacaa cattacgtag cgggttaggt ggtgtactga tgggcttcgg
      901 tgccagcatt gcaggtggtt gctctatcgg taatggactg gtcatgactg caatgatgac
      961 ctggcagggc tggattggcc ttgtatttat gattctcgga gtctggactg cgtcctggct
     1021 tgtgtatgtt cgaccgcagc gtaaggcacg actggcaact gctgcggcaa attaagtaag
     1081 gtgtaaagat ggcgattaaa aagctggatg tggtgacgca ggtctgtccg tttccactca
     1141 tagaagcaaa agcagcactg gcagaaatgg ccagcggcga tgagttggta attgagtttg
     1201 attgcaccca ggcaactgaa gcaatcccac agtgggcggc agaagaagga catgccatca
     1261 ccgattatca gcagattggc gatgcagcgt ggagcatcac cgttcaaaaa gcctgactca
     1321 aaattgtcct ccgccgtacc agcggcggag gcttcacatt agacaatctc ttccgcgtac
     1381 tgccaaagcg cttttaacag cgccactttt tctttatcgt cggcatactg ctgcgccagc
     1441 atctgaatct cttctgcata gccttgtaaa aactctggcg tgaagacctg gcggcggtgc
     1501 tccagccagc gttgctgctc ggcataatcc agcgtccccg ggaagttgcg cgcccgataa
     1561 ttgaacaaca gcttttcaat tcgtttatca acaaaagtga tatccagtgc cggtaaatta
     1621 cgcggctcgg tttccagcac aattttcatt gctgcacgat cggcgtcact gaaaaagccg
     1681 ttataaagct gtgcatccac attatctgaa ggtgtaaacg gttcggcttc cacgaatatc
     1741 gctaccactt tttcacgcac ttgcggattt tcacgcagaa tttttaggtt atcgaggcaa
     1801 tgctgacgat taattcccag tcggtcggca tcttccgggc gtagcgtatt cgcctgggcc
     1861 agtaccggac atttattgat atgtaccagc ttaaccggaa cggcggcgtt atcgccaaga
     1921 tcggcttttg cggtatataa acgctcgcgc aatgtgtcgc tatccagctc cagtaatggc
     1981 gaaatgtctc ctgccaaatc caccataatt acggcattgc gattttcagg atgccacgcc
     2041 agcggtgcca cccagcttgt attgccgcgc catgcaccaa acattccgga aacgtgaacc
     2101 aggggtttca tctgaggaac atcaatcaac gccattagtt tgtgtttatt acgatgggta
     2161 aagagataat caaacaggcg tggctgacgc gtttttacca gttttgccat cgcaatagtg
     2221 gcgtacacat cagccatcgc atcgtgggcg ttgctatgtt caataccatt cgctttggtt
     2281 aaatgctcaa ggcgaaagct cggtagaccg tcatcatttt caggccaatt tattccttcc
     2341 gggcgcaggg cataacaggc acgcataaca tccagtaaat cccagcgcga gttatcatgc
     2401 tgccagctcc aggcgtaagg atcgtagaaa ttacgataaa aaacgttgcg tgtgacttcg
     2461 tcgtcgaaac gcacattgtt gtagcccaga atacaggtct tcggtacggt aaaaagcgag
     2521 tgaatacggg cggcaaacgc ggcttcgttt tctcctttcg ctcgtgtctc ctgcggggta
     2581 ataccggtaa ttaatactgc tccaggctgg ggtaaatagt catccgcggg cttgcagtaa
     2641 aagacttcgg gttcgccgat gacattgaat tcgtcatcgg tgcgaatggc tgcgaactgt
     2701 gcagggcgat ctaacgcggg gtgcgtgcca aaggtttcgt aatcgtgaaa caaaaaggta
     2761 gattgttgct taccgtcatt catcattagg ttaaatccgt tatttctgct gtctgccaga
     2821 gtatcaaata tcaccgtgct aatcagcttt agcgcgacaa tttgacagcg agtgacaaca
     2881 gatcatgtca gataaaaatg agagggtagt cacattttct tgcactttat tccagccagt
     2941 tcataagtat ttccgtaaaa agaacagcta tttgaaactc ctgagggttt gctgttgaaa
     3001 cgccgtctta ttattgctgc ttctttgttc atttttaact tatcgtctgg ttttgcggcg
     3061 gaaaacattc ctttttcacc tcagcctcca gagattcatg ccggctcctg ggtactgatg
     3121 gattacacca ccggtcagat cctcaccgcg ggtaatgagc atcaacagcg caatcccgcc
     3181 agcctgacaa agctgatgac gggctatgtc gtggatcgca ctatcgatag tcatcgcatt
     3241 acgccagacg atattgtcac cgtggggcgc gatgcgtggg cgaaagataa tccagtgttt
     3301 gtcggttctt cactgatgtt tttgaaagag ggcgatcgcg tatcggtacg tgatttaagc
     3361 cgtggtttaa ttgtggattc cggaaatgac gcttgtgttg cactggctga ctatattgcc
     3421 ggtgggcaac ggcagtttgt tgaaatgatg aacaactatg ccgagaagct gcatctcaag
     3481 gatacgcatt ttgaaacagt gcatggtctg gatgcaccag gtcagcatag ctcggcttat
     3541 gatttagctg tgctttctcg cgctatcatc cacgacgagc ccgagtttta tcatatgtac
     3601 agtgagaaaa gtctcacctg gaacggtatc acccagcaaa accgtaacgg gttattgtgg
     3661 gataaaacca tgaatgttga cggcctgaaa acgggtcata cttctggtgc cgggtttaat
     3721 ctcattgctt cggctgtcga tgggcagcgt cgtctcattg cagtggtaat gggggctgac
     3781 agtgcaaaag gtcgtgagga agaggcaaga aaattactgc gttgggggca acaaaacttt
     3841 actacggtgc aaattttgca ccgtgggaaa aaggtcggta cggaacgcat ctggtatggc
     3901 gataaagaaa atatcgacct gggaacggaa caagagttct ggatggtgct accgaaagcc
     3961 gaaattccac atatcaaagc caaatatacc cttgatggta aagagctcac cgcgccaatt
     4021 agcgcccatc agcgggtagg ggaaattgaa ctttacgacc gtgataaaca ggtggcgcac
     4081 tggccgctgg ttaccctgga atctgtcggg gaaggcagca tgttttctcg cctgagtgat
     4141 tatttccacc ataaggcctg acctttcttt tgcagcagac tggcaggagt gcgagtctgc
     4201 tcgcataatc aacactcatt ccttgtggtt ttaatatcgc aat
//
LOCUS       DAZKHF020000305         4209 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2201S31241
            SAMN43267800-rid25106413.denovo.307, whole genome shotgun sequence.
ACCESSION   DAZKHF020000305 DAZKHF020000000
VERSION     DAZKHF020000305.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267800
            Sequence Read Archive: SRR30307263
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4209)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4209)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 4209)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350581.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350581.2
            Genome Coverage       :: 151x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:21:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,652
            CDSs (total)                      :: 5,557
            Genes (coding)                    :: 5,320
            CDSs (with protein)               :: 5,320
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 237
            CDSs (without protein)            :: 237
            Pseudo Genes (ambiguous residues) :: 0 of 237
            Pseudo Genes (frameshifted)       :: 102 of 237
            Pseudo Genes (incomplete)         :: 130 of 237
            Pseudo Genes (internal stop)      :: 52 of 237
            Pseudo Genes (multiple problems)  :: 41 of 237
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4209
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267800-rid25106413.denovo.307"
                     /serotype="O 26:H11"
                     /isolate="2201S31241"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-01"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            35..607
                     /gene="lpfA-O113"
                     /locus_tag="ACHOSC_RS21805"
                     /old_locus_tag="pgaptmp_004361"
     CDS             35..607
                     /gene="lpfA-O113"
                     /locus_tag="ACHOSC_RS21805"
                     /old_locus_tag="pgaptmp_004361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long polar fimbria major subunit LpfA-O113"
                     /protein_id="HGU4550162.1"
                     /translation="MKRNIIGGAFTLASLMLAGHALAEDGVVHFVGEIVDTTCEVTSD
                     TADQIVPLGKVSKNAFSGVGSLASPQKFSIKLENCPATYTQAAVRFDGTEAPGGDGDL
                     KVGTPLTAGNPGDFTGTGQAIAATGVGIRIFNQSDNSQVKLYNDSAYTAIDAEGKAEM
                     KFIARYVATNATVTAGTANADSQFTVEYKK"
     gene            655..1386
                     /gene="lpfB-O113"
                     /locus_tag="ACHOSC_RS21810"
                     /old_locus_tag="pgaptmp_004362"
     CDS             655..1386
                     /gene="lpfB-O113"
                     /locus_tag="ACHOSC_RS21810"
                     /old_locus_tag="pgaptmp_004362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_316045823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long polar fimbrial chaperone LpfB-O113"
                     /protein_id="HGU4550163.1"
                     /translation="MSRKSCLIRLFKTTVSCKLFTAIILSVFLGQPALTYAGVVIGGT
                     RVVYLSNNADKSISVFSKEEKIPYLIQAWVDPFNKEDKSKAPFTVIPPVSRLEPSQEK
                     ILRIIHTKGVSLPDDRESVFWLNIKNIPPSASNKATNSLEIAVKTRIKLFWRPANIRL
                     IPEDAAPKVKWRREGRNLIAENPNPIHISVMDVIVDGHDVPLNMIRPFETLTLPLPAN
                     SAGGQMTWRFINDYGAVSDPIKMTL"
     gene            1411..3933
                     /gene="lpfC-O113"
                     /locus_tag="ACHOSC_RS21815"
                     /old_locus_tag="pgaptmp_004363"
     CDS             1411..3933
                     /gene="lpfC-O113"
                     /locus_tag="ACHOSC_RS21815"
                     /old_locus_tag="pgaptmp_004363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long polar fimbrial usher protein LpfC-O113"
                     /protein_id="HGU4550164.1"
                     /translation="MMTTRIVVGLTAGTCLIFSQNLMAEVSVFNPALLEIDHQSGVDI
                     RQFNRANLMPPGVYSVDIFINGKMFERQDVTFVQDNPDADLHACFIAIKKTLSSFGIK
                     VDALKSFNDVDETVCLDPAPRIEGSSWQFDSDKLQLNISIPQIYMDAMAYDYISPTRW
                     DEGINALTINYDFSGSHTLRSDYGSQETDTSYLNLRNGLNIGPWRLRNYSTLNTSDGR
                     AEYNSISTWIQRDIAALRSQIMIGDTWTASDIFDSTQIRGARLYTDNDMLPASQNGFA
                     PVVRGIAKSNATVIIRQNGYVIYQSAVPQGAFEITDLNTASTGGDLDVTIKEEDGSEQ
                     RFTQPYASLAILKREGQTDVDVSVGELRDEDGFTPDVLQAQILHGFSHGITLYGGMQA
                     AENYGSAALGVGKDLGALGAISFDVTHARANFSHDDTETGQSYRFLYSKRFDDTDTSL
                     RLVGYRYSTEGYYTLNEWASRRNSPEDFWETGNRRSRVEGTLTQSLGRDYGNLYLTLS
                     RQQYWHTDDVERLMQFGYSSSWKRLSWNVSWSYSNTARQGTGNNHASDNTSEQIYMLS
                     LSVPLSGWWGNSYATYSVSQNDNSGSSHQLGLSGTALERNNLSWNLMQSYNSHDDEVG
                     GNMSLTYDGSYGTVNGSYNYSQNSQRLNYGIRGGILAHSEGVTLSQELGETIALVKAP
                     GAAGLEIDNMRGAATDWRGYTVKTQLNPYDENRVAISDNYFSKSNIELDNTVVTMVST
                     RGAVVKAEFVTHVGYRVLFRVLNANGKPVPFGAIAAIQDASLADSGIVGDRGELYLSG
                     LPEKGQVTLSWGENASTKCIFNYSLSTPESESGLIEQGVTCH"
     gene            3944..>4209
                     /locus_tag="ACHOSC_RS21820"
                     /old_locus_tag="pgaptmp_004364"
     CDS             3944..>4209
                     /locus_tag="ACHOSC_RS21820"
                     /old_locus_tag="pgaptmp_004364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001685709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HGU4550165.1"
                     /translation="MNKYIKQWCFAVFMLSLSSVALAAPKGICTPDNGVFHSTLDFSG
                     YLITANENKVGTTFNTTVTNGSSYPGRCHCDTGNVGEFPYIYYTS"
BASE COUNT         1146 a          904 c         1024 g         1135 t
ORIGIN      
        1 atggtaaagg atatattata tcaatggagt ttttatgaag cgtaatatta taggcggtgc
       61 attcactctg gcatctctaa tgctggccgg gcatgcactg gcagaagatg gtgttgttca
      121 cttcgtcggt gaaattgtcg acactacttg tgaagttacc tccgatacag ccgatcaaat
      181 tgtcccactg ggtaaagtca gtaaaaatgc attttcaggt gtaggtagtc tggcgtcgcc
      241 acagaagttc agtattaagc ttgaaaattg cccggcaacg tacactcaag cagccgttcg
      301 ttttgatggt acagaagcgc ctggcggcga cggcgacctg aaagtgggta cgccgcttac
      361 agcaggcaac cctggtgatt ttaccggtac aggacaagcg attgcggcaa ccggcgttgg
      421 tattcgtatt tttaaccagt ccgataattc gcaggttaaa ctttataacg actctgctta
      481 taccgctatc gatgctgaag gcaaggctga aatgaagttt attgcacgct atgtggcaac
      541 caatgcgacc gtaacggctg gtacggcgaa cgctgattca caatttactg tcgaatataa
      601 gaaataattt atcaagggcc tttttattca gttggctttg gccagggagt cgttatgtcc
      661 agaaaaagtt gtttaatcag gctctttaaa acaacagttt catgtaagtt atttaccgcg
      721 attatactca gcgttttttt aggccaacca gcattaactt atgcaggcgt tgtcattggt
      781 ggtacgcggg tggtttactt aagtaataat gctgataaat ctatttctgt atttagcaaa
      841 gaagaaaaaa taccttatct catccaggct tgggtagatc catttaataa agaggataaa
      901 agcaaagcgc cgtttaccgt tattccgcca gtatcacgat tagaaccttc gcaagaaaaa
      961 atattacgca ttattcacac caaaggtgtg tcattgcctg atgatcgcga gtcagtcttc
     1021 tggctgaata ttaaaaatat tccgccatca gcgagcaaca aagcgacgaa ctcgctggaa
     1081 atagcagtaa aaacgcgtat caagctgttc tggcgaccag caaatatccg ccttattccc
     1141 gaagatgctg cgccgaaagt gaaatggcgc cgggaagggc gaaatctgat agcagagaat
     1201 cccaacccaa ttcatatcag tgtgatggat gtcatcgttg atggccatga tgtgcccctc
     1261 aatatgatcc gtccttttga aacgctcacg ttacctttac ctgctaattc tgcgggaggc
     1321 caaatgacct ggcgatttat taatgattac ggcgcagtca gtgacccgat aaaaatgacg
     1381 ctgtaagcaa gggataagga taggcgtatt atgatgacga ccagaatagt ggttggcctc
     1441 acggcaggga cgtgtctgat tttctcgcaa aacctgatgg ccgaggtcag tgtattcaat
     1501 ccggcgcttc tggaaatcga ccatcaatcc ggagtcgata ttcgccagtt taatcgggca
     1561 aacctgatgc ccccaggtgt ttatagcgtt gatattttta tcaacggtaa aatgtttgaa
     1621 cgtcaggatg tgacatttgt tcaggataat ccagatgctg atctgcacgc ttgctttatt
     1681 gccattaaaa aaacactgtc ctcctttggc ataaaagttg atgcgctcaa atcgttcaat
     1741 gatgtggatg agacggtttg cctcgatcct gctccacgta ttgaaggctc atcctggcag
     1801 tttgacagtg ataaattgca gctgaatata tccattcccc aaatctacat ggacgcgatg
     1861 gcttatgatt acatcagccc cacgcgttgg gatgagggga ttaacgcgct caccatcaac
     1921 tacgattttt ctggttcaca tacactacgt tcagattatg gttcacaaga gacagatacc
     1981 agttatctca atctgcgcaa tggactgaat attggaccgt ggcggctacg taattatagt
     2041 actttaaaca ccagcgatgg ccgtgcggaa tacaactcca ttagtacctg gatacagcgc
     2101 gatattgccg cgttaagaag ccagattatg attggtgata cgtggacggc gagcgatatt
     2161 ttcgacagta cgcaaattcg cggcgcgcgt ttgtatactg ataacgatat gctacccgcc
     2221 agccagaatg gctttgctcc tgtggttcgt gggattgcaa agtccaacgc caccgtcatc
     2281 attcggcaga atggctacgt gatttatcag tctgccgttc cacaaggtgc ttttgagatc
     2341 accgatctca acaccgccag tacaggtggc gatttggacg taaccatcaa agaagaagac
     2401 ggtagcgaac aacgattcac ccaaccttat gcttcattgg cgattcttaa acgtgaaggt
     2461 cagacagatg ttgatgtcag cgtgggtgaa ttgcgcgatg aagacggatt tacaccggac
     2521 gtccttcagg cgcaaatact tcatggtttt tcccacggga tcactttata tggaggtatg
     2581 caggctgctg aaaattatgg ttctgcagct ctgggtgtcg gtaaagatct tggcgctttg
     2641 ggcgcaattt ctttcgatgt gacacatgct cgtgcgaatt ttagccatga tgatacagaa
     2701 acgggtcagt catatcgctt tctctattca aaacgatttg acgacacaga cactagcttg
     2761 cgcctggttg gctatcgtta ctccaccgag ggctactata ccctcaatga gtgggcatcg
     2821 cggcgcaaca gccctgaaga cttttgggaa acaggtaacc gacgtagtcg cgtggaggga
     2881 acgctaacgc agtcgttggg gagagattat ggcaatttat acctgacatt aagccggcaa
     2941 caatactggc ataccgatga tgtcgaacga ttaatgcaat ttggctacag cagtagctgg
     3001 aagcgtctct cgtggaacgt ctcctggagt tattccaata ctgccagaca ggggacgggg
     3061 aacaaccatg ccagtgataa caccagtgag cagatctaca tgctctcttt atctgttcct
     3121 ttatcgggct ggtggggtaa tagttacgcc acctattctg tttcgcaaaa cgataattcc
     3181 ggtagctcac atcaactcgg actcagcggt acggcgctgg aacgaaataa cctttcatgg
     3241 aatttaatgc agtcctataa cagtcatgat gatgaggttg gcggtaatat gtccctgacc
     3301 tatgatggct cttatggcac ggtgaacggc agctataact acagccaaaa ttcccagagg
     3361 ctgaattatg gtatcagagg gggaattctg gcacacagcg aaggggtaac gttaagtcag
     3421 gagttaggtg aaactattgc tcttgttaaa gcacctgggg ccgccgggtt agaaatagat
     3481 aatatgcgcg gtgctgcgac ggactggcgc ggctatacgg tcaagacaca gctaaaccct
     3541 tatgatgaaa atcgggtagc aatcagcgat aactatttct cgaagtcgaa tatagaactt
     3601 gataataccg tcgttacgat ggtttccacg cgtggtgcag tggttaaagc ggagtttgtg
     3661 actcatgtgg gttatcgcgt tctcttcaga gtgttaaatg caaatggtaa accggtacct
     3721 tttggagcca ttgctgcgat acaagatgca agtttggcag attcaggaat tgtcggtgac
     3781 cgtggcgaac tttatctttc tggtctacca gaaaaaggac aggttacgtt atcctgggga
     3841 gaaaacgcct caacaaaatg catcttcaat tattcacttt cgacaccaga aagtgagagc
     3901 ggattaattg aacagggtgt gacatgtcat taaaaggatt tttatgaaca agtatatcaa
     3961 acagtggtgc tttgctgtgt ttatgctctc gttaagtagt gtagcacttg cagctcctaa
     4021 aggtatctgt acccctgata atggcgtatt tcacagtacg cttgatttct cgggatatct
     4081 tattaccgcg aacgaaaata aggttggaac gacatttaac accactgtaa ctaatggatc
     4141 ctcatatcct ggacgctgtc attgtgatac tggtaacgta ggagaattcc cctatatcta
     4201 ttatacgtc
//
LOCUS       DAZKHF020000306         4202 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2201S31241
            SAMN43267800-rid25106413.denovo.308, whole genome shotgun sequence.
ACCESSION   DAZKHF020000306 DAZKHF020000000
VERSION     DAZKHF020000306.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267800
            Sequence Read Archive: SRR30307263
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4202)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4202)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 4202)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350581.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350581.2
            Genome Coverage       :: 151x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:21:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,652
            CDSs (total)                      :: 5,557
            Genes (coding)                    :: 5,320
            CDSs (with protein)               :: 5,320
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 237
            CDSs (without protein)            :: 237
            Pseudo Genes (ambiguous residues) :: 0 of 237
            Pseudo Genes (frameshifted)       :: 102 of 237
            Pseudo Genes (incomplete)         :: 130 of 237
            Pseudo Genes (internal stop)      :: 52 of 237
            Pseudo Genes (multiple problems)  :: 41 of 237
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4202
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267800-rid25106413.denovo.308"
                     /serotype="O 26:H11"
                     /isolate="2201S31241"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-01"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            complement(116..2449)
                     /locus_tag="ACHOSC_RS21825"
                     /old_locus_tag="pgaptmp_004365"
     CDS             complement(116..2449)
                     /locus_tag="ACHOSC_RS21825"
                     /old_locus_tag="pgaptmp_004365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003896 - DNA primase activity [Evidence
                     IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="primase-helicase zinc-binding domain-containing
                     protein"
                     /protein_id="HGU4550166.1"
                     /translation="MSGMKVSQAVKAARGHWAQILPALGVNILKNRHQPCPVCGGKDR
                     FRFDDQEGRGTWFCNQCGAGDGLALVTRALNVDISEAADRIHGLTHDLSVVNSEVRTL
                     TADTDSGKDAAAALAARLLQASRESAGNTYLTHKGFPEHICHELTSAHKAGGMMFRPG
                     DLIVPLYNADGELVNIQLISGNGSKCFLKGGQVKEAYHLIEGGGSSVRRIWIAEGYAT
                     ALTIHYLTGEAVMVAFSSVNFLSLSSVAHNKYPGYQLIIAADRDLNGSGQNRAETAAK
                     ASQCDIVLPPVFGDWNDALTQYGEESTRKAILEALKPHNASPFDTMSEAEFTAMSVSE
                     KAQRVREHYRDALAVDPNGQLLSRYESGAWKVISQSDFARDVAALFQRLGAPFSSGKI
                     ASLVETLKLIVPQQQNPSRHLIGFRNGVLDTRTGLFSPHCKENWLRTLCEVDFTPPVK
                     GETLETHAPAFWRWLDRAAGHKPAKRDIILAALFMVLANRYDWQLFLEVTGPGGSGKS
                     ILAEIATMLAGEDNATSATIETLESPRERAALIGFSLIRLPDQEKWSGDGAGLKAITG
                     GDAVSVDPKYQNAYSTHIPAVILAVNNNPMRFTDRSGGVSRRRVILHFPEQIAPEERD
                     PQLKDKIARELAVIVRQLMQRFSDPMSARTLLQSQQNSDEALTIKRDADPAFDFCGYL
                     EALPDTNGMFMGNANIVPRQPRTYLYHAYLVYMEANGYKNTLSLTMFGKGLPVMLKEY
                     GLHYEKRRTNQGMQTNLTLKEESNADWLPKCDEPILK"
     gene            complement(2464..2787)
                     /locus_tag="ACHOSC_RS21830"
                     /old_locus_tag="pgaptmp_004366"
     CDS             complement(2464..2787)
                     /locus_tag="ACHOSC_RS21830"
                     /old_locus_tag="pgaptmp_004366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016809712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5375 family protein"
                     /protein_id="HGU4550167.1"
                     /translation="MKSRTLSLSLRVALYRRAVACAWLSACYQQNRHLQLTLDEIETA
                     IARELEGFYLRQHGQTKGMEIACALLSDLMEYGPLMSCPALSQLGIAVMDELCVRHIK
                     KPVLH"
     gene            complement(2784..3008)
                     /locus_tag="ACHOSC_RS21835"
                     /old_locus_tag="pgaptmp_004367"
     CDS             complement(2784..3008)
                     /locus_tag="ACHOSC_RS21835"
                     /old_locus_tag="pgaptmp_004367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014882693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4550168.1"
                     /translation="MNHAAISYDDILRLKHLRNVGEFVTGMAVLQDCYEKPASAQCEQ
                     LVSLIYLMTEQLDGVVQRCQDDLMNMEVVQ"
     gene            complement(3008..3556)
                     /locus_tag="ACHOSC_RS21840"
                     /old_locus_tag="pgaptmp_004368"
     CDS             complement(3008..3556)
                     /locus_tag="ACHOSC_RS21840"
                     /old_locus_tag="pgaptmp_004368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014882692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="host cell division inhibitor Icd-like protein"
                     /protein_id="HGU4550169.1"
                     /translation="MKLALQSHLLFSGLRPDIFSRYSFPAAAKSAAGRRNPSYLLATP
                     DAPCVFFYVVAQAHPFFGLRCLYLSPFQIMVVRAGQPSGWPVSNKAGIPTPVRVTTHE
                     RRNSGGGIYRYLLEVALMATILAPSHPQFIFVFAAVRRAERKPRISMLRIIASNEHHA
                     RLSLVHEYVLCLAARLPVREVI"
     gene            complement(3553..3813)
                     /locus_tag="ACHOSC_RS21845"
                     /old_locus_tag="pgaptmp_004369"
     CDS             complement(3553..3813)
                     /locus_tag="ACHOSC_RS21845"
                     /old_locus_tag="pgaptmp_004369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HGU4550170.1"
                     /translation="MNSISVSTPAPAAPVLPVPAQQERFIRLPEVMHLCGLSRSTIYD
                     LISRDAFPQQISLGGKNVAWVQSEVSAWMAARIAAREQGGHA"
BASE COUNT         1116 a         1093 c         1002 g          991 t
ORIGIN      
        1 ttttaactta tatatcaatt gtttacgtgt taacagcgag gtgaagagtg aacagctatc
       61 tctacatttg tgcgtttcca ataagaagcc cggcatcagc cgggttagtt aatcgttatt
      121 ttaatattgg ctcatcacac ttgggcagcc agtctgcatt actctcctct ttcaaagtga
      181 ggttagtttg cattccctga ttagttcgcc gtttctcata atgtagcccg tactccttca
      241 gcatgactgg caaccctttg ccaaacatcg taagactcag ggtgttttta tagccattag
      301 cctccatgta tacgaggtag gcatggtaaa ggtatgtacg tggctggcgt gggacaatat
      361 tggcgttccc cataaacata ccgttggtgt caggtaatgc ctcaaggtag ccacaaaaat
      421 caaatgctgg atcagcatca cgcttgatgg tgagagcctc atcagaattc tgctgcgact
      481 gaagcaatgt tctggcgctc atcggatcgc tgaaacgctg catgagctga cgaacaatca
      541 cagccagttc tcgtgcaatt ttatctttca actgcggatc gcgttcttcc ggtgctatct
      601 gctcaggaaa atgcagaata actctcctgc gggacacacc accactgcga tcagtgaagc
      661 gcataggatt attatttacg gccaagataa ccgccggaat atgtgttgaa taagcattct
      721 gatatttggg gtccacagat actgcatcgc caccagttat agccttaagc ccggcgccgt
      781 cgccgctcca cttctcctga tcaggcaggc gaataagaga aaaacctatc agagccgcac
      841 gttctcgcgg ggactccagc gtttcaatgg tcgcagaggt ggcgttatcc tctccagcaa
      901 gcatggtcgc aatttcagcc agaatacttt tgccacttcc accagggcca gtcacttcca
      961 gaaagagctg ccagtcatag cggttcgcca gcaccataaa taacgctgca agaataatgt
     1021 cgcgttttgc tggcttgtgt ccggcggccc ggtccagcca acgccagaat gccggggcat
     1081 gagtttcaag cgtttccccc tttaccggtg gcgtgaaatc gacttcacac agggtacgca
     1141 gccagttctc tttacagtgc ggactaaaaa gccctgttcg cgtatcgaga acgccattac
     1201 gaaaaccaat cagatgacgt gacggatttt gctgctgcgg aacaattaac tttaatgttt
     1261 ccaccagtga ggcaattttt cctgaggaaa acggtgcccc gaggcgctgg aaaagggctg
     1321 cgacatctcg ggcaaaatct gactgagaaa taactttcca cgctccagac tcgtagcggg
     1381 ataaaagctg cccgttcgga tcaaccgcca gtgcatccct gtaatgctcc cgaaccctct
     1441 gagctttttc gctgacactc atcgctgtga attctgcttc actcattgtg tcgaaaggac
     1501 tggcgttatg tggcttcaaa gcctcaagaa ttgccttccg ggtggattcc tctccgtatt
     1561 gggtaagcgc atcattccag tcaccaaaaa ccggtggcag cacaatgtca cactgactcg
     1621 cttttgcagc agtttcagcc ctgttctggc ctgaaccatt cagatcgcgg tccgctgcaa
     1681 taattaactg atatcccgga tacttattat gggcaacgct gctcagagaa agaaagttga
     1741 ccgacgaaaa tgccaccatg acggcttctc ctgtcaaata atgaatggta agcgccgtgg
     1801 cgtaaccttc cgcaatccaa atccttctta cagaactccc gcccccttct atcagatgat
     1861 aggcctcctt aacctgacct cctttaagaa aacacttgct accattgcca ctgatgagct
     1921 gaatattcac cagctccccg tcagcgttat agagtgggac gatcaaatca ccggggcgga
     1981 acatcatacc accggcctta tgagctgaag tcagctcatg gcaaatatgc tccgggaaac
     2041 ctttatgtgt aagataagtg tttccggcag attcacgcga ggcttgcagc agtcgcgcgg
     2101 caagtgctgc cgctgcatct ttcccgctat cggtatcggc ggttaacgtc ctgacctcgg
     2161 aattaactac agacagatca tgtgtcagtc catgtatcct gtcagctgct tcactgatat
     2221 ccacattcag tgccctggtg acaagcgcta aaccatcccc ggctccgcac tgattgcaga
     2281 accatgttcc gcgtccttcc tgatcgtcga agcgaaaacg atctttaccg ccacagaccg
     2341 ggcaaggctg atggcgattt ttcaatatat tcacgcccag cgctggcaga atctgagccc
     2401 agtgaccgcg ggcagccttc acggcctgac tgactttcat tcctgacatg acgcagttct
     2461 ccctcagtgt aaaaccggct ttttgatgtg acggacgcat aactcatcca ttacggctat
     2521 tccgagctgg gagagtgcgg gacaggacat tagagggccg tattccatca ggtctgaaag
     2581 cagggcgcag gcgatttcca tgccttttgt ttgcccgtgc tgtcgcagat aaaatccttc
     2641 aagctcacgg gcaatagcgg tttcaatttc atccagagtg agctgcagat ggcggttttg
     2701 ctgatagcag gcactcaacc aggcgcaggc taccgcgcgg cgatacaacg ctacccgaag
     2761 tgaaagggaa agagtgcgtg atttcattgc accacctcca tgttcatgag gtcatcctgg
     2821 caacgctgta ccacgccatc aagctgctct gtcatcagat aaatcaggga aaccagttgt
     2881 tcacattgag cactggcagg tttttcgtag caatcctgaa gaacagccat tccagtgaca
     2941 aactctccca cgttacgaag atgcttcagg cggagaatat cgtcataaga gattgcggcg
     3001 tgattcatta gatcacctcc cggacaggca ggcgagcagc aagacaaagc acatactcat
     3061 ggaccagtga aagacgtgca tgatgctcat tgctggcgat gatacgcagc atactgatac
     3121 gtggtttacg ttctgcacga cgaacggcgg caaaaacaaa tataaattga ggatgtgacg
     3181 gggcgaggat cgtagccata agggcaacct ccaataagta gcggtaaatg ccaccaccgg
     3241 agttcctacg ctcatgggtg gtgacccgaa cgggggtagg aataccggcc ttattggaaa
     3301 ccggccagcc cgaaggctgc cccgcccgga ccaccattat ctgaaagggg ctaaggtata
     3361 agcaccgcag cccgaaaaat gggtgtgcct gagcaacgac gtaaaaaaag acgcacggcg
     3421 cgtctggtgt cgccaataag taactcgggt tcctacgccc ggcagccgat tttgcggcag
     3481 cgggaaaact atacctggaa aagatatcag gacgcaagcc agaaaaaaga agatgagact
     3541 gcaaagcaag tttcacgcat ggcctccttg ctcgcgggca gcaattcgcg ccgccatcca
     3601 tgcactgacc tccgactgta cccaggccac attttttcca cccagggata tctgctgcgg
     3661 gaaggcatcg cggctgataa ggtcgtagat ggttgaacgg gatagcccgc aaaggtgcat
     3721 cacttcaggt aatcgtatga agcgctcctg ctgagccgga accgggagca ccggagcagc
     3781 tggtgccggg gtggaaacgg aaatactatt catctggcta cctctctaat atgtttacaa
     3841 cagtccgggc aattccatgc ggattcaggt agctccttat tatgtttata taagcggctc
     3901 gcgcatgctt tatttatttt gtgtgaaatg ttgatttctc aaaaaataaa acagctaaaa
     3961 accgtatgaa acgaacaaaa acaaacaatt cctttatgga atataataag gagcatcaat
     4021 aataaaaaag tctattacac taggcaggat cacagctcaa atctgaacca gcccatatca
     4081 ttcagggcgt tacatcataa gcactaagac aacacctgat taataaaaga gccaaaacgg
     4141 ccaaaaataa acagctcacc atatgtatca ataaatccga tgattaaaga aattaaatta
     4201 cc
//
LOCUS       DAZKHF020000307         4199 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2201S31241
            SAMN43267800-rid25106413.denovo.309, whole genome shotgun sequence.
ACCESSION   DAZKHF020000307 DAZKHF020000000
VERSION     DAZKHF020000307.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267800
            Sequence Read Archive: SRR30307263
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4199)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4199)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 4199)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350581.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350581.2
            Genome Coverage       :: 151x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:21:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,652
            CDSs (total)                      :: 5,557
            Genes (coding)                    :: 5,320
            CDSs (with protein)               :: 5,320
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 237
            CDSs (without protein)            :: 237
            Pseudo Genes (ambiguous residues) :: 0 of 237
            Pseudo Genes (frameshifted)       :: 102 of 237
            Pseudo Genes (incomplete)         :: 130 of 237
            Pseudo Genes (internal stop)      :: 52 of 237
            Pseudo Genes (multiple problems)  :: 41 of 237
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4199
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267800-rid25106413.denovo.309"
                     /serotype="O 26:H11"
                     /isolate="2201S31241"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-01"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            complement(136..708)
                     /locus_tag="ACHOSC_RS21850"
                     /old_locus_tag="pgaptmp_004370"
     CDS             complement(136..708)
                     /locus_tag="ACHOSC_RS21850"
                     /old_locus_tag="pgaptmp_004370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001774583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage polarity suppression protein"
                     /protein_id="HGU4550171.1"
                     /translation="MESTALQQAFDTCQNNKAAWLQRKNELAAAEQEYLRLLSGEGRN
                     VSRLDELRNIIEVRKWQVNQAAGRYIRSHEAVQHISIRDRLNDFMQQHGTALAAALAP
                     ELMGYSELTAIARNCAIQRATDALREALLSWLAKGEKINYSAQDSDILTTIGFRPDAA
                     SVDDSREKFTPAQNMIFSRKSAQLASRQSV"
     gene            complement(782..1282)
                     /locus_tag="ACHOSC_RS21855"
                     /old_locus_tag="pgaptmp_004371"
     CDS             complement(782..1282)
                     /locus_tag="ACHOSC_RS21855"
                     /old_locus_tag="pgaptmp_004371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001405508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ogr/Delta-like zinc finger family protein"
                     /protein_id="HGU4550172.1"
                     /translation="MIYCPSCGHVAHTRRAHFMDDGTKIMIAQCRNIYCSATFEASES
                     FFSDCKDSGMEYISGKQRYRDSLTSASGSMKRPKRMLVTGYCCRRCKGLALSRTSRRL
                     SQEVTERFYVCTDPGCGLVFKTLQTINRFIVRPVTPDELAESLHEKQELPPVRLKTQS
                     YSLRLE"
     gene            complement(1279..2013)
                     /locus_tag="ACHOSC_RS21860"
                     /old_locus_tag="pgaptmp_004372"
     CDS             complement(1279..2013)
                     /locus_tag="ACHOSC_RS21860"
                     /old_locus_tag="pgaptmp_004372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001600374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoprotein 3"
                     /protein_id="HGU4550173.1"
                     /translation="MSDNTIPEYLQPALAQLEKARAAHLENARLMDETVTAIERAEQE
                     KNALAQADGNNADDWRTAFRAAGGVLSDELKQRHIERVARRELVQEYDNLAVVLNFER
                     ERLKGACGSTATAYRKAHHHLLSLYAEHELEHALNETCEALVRAMHLSILVQENPLAN
                     TTGHQGYVAPEKAVMQQVKSSLEQKIKQMQISLTGEPVLRLTGLSAATLPHMDYEVAG
                     TPAQRKVWQDKIDQQAAELKARGLLS"
     gene            2565..2831
                     /locus_tag="ACHOSC_RS21865"
                     /old_locus_tag="pgaptmp_004373"
     CDS             2565..2831
                     /locus_tag="ACHOSC_RS21865"
                     /old_locus_tag="pgaptmp_004373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015979398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HGU4550174.1"
                     /translation="MQAVFSSPSPAPVTPLMPLPDITQERFLRLPEVMHLCGLSRSTI
                     YELIRKGEFPPQVSLGGKNVAWLHSEVTAWMAGRIAGRKRGYDA"
     gene            2828..3427
                     /locus_tag="ACHOSC_RS21870"
                     /old_locus_tag="pgaptmp_004374"
     CDS             2828..3427
                     /locus_tag="ACHOSC_RS21870"
                     /old_locus_tag="pgaptmp_004374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000980235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="host cell division inhibitor Icd-like protein"
                     /protein_id="HGU4550175.1"
                     /translation="MMMPALQKLPFSGLPFSGICGYSFSAAAKSAAGRENPSNSQATY
                     DAPRVFFCVAINATERQIMVWCVVSRADGIPCILPASAHYAAESMVAQAGQPPGWPVS
                     CEAGILTPVWAIAIERENSGDSVICYSQEAAIMATTLTPSHPEFVFVFAAVRRADRHP
                     RICMLRTVAGDERSARRSLVRDYVLSLAARLPVVEVSRA"
     gene            3420..3707
                     /locus_tag="ACHOSC_RS21875"
                     /old_locus_tag="pgaptmp_004375"
     CDS             3420..3707
                     /locus_tag="ACHOSC_RS21875"
                     /old_locus_tag="pgaptmp_004375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001244665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Derepression protein"
                     /protein_id="HGU4550176.1"
                     /translation="MRNKKAPQTVSARHDAREHLSIEAYHKLNRASAVSQFVGGDLIH
                     RELSGLHQLYIPHIFSYLNEDIDFVLNELKAKGLCRDFLAQQKDRGDRTHV"
     gene            3700..4155
                     /locus_tag="ACHOSC_RS21880"
                     /old_locus_tag="pgaptmp_004376"
     CDS             3700..4155
                     /locus_tag="ACHOSC_RS21880"
                     /old_locus_tag="pgaptmp_004376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016569899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4550177.1"
                     /translation="MFDFPRPGEIYRSAGFPDVAVVGILEDGIPWEMPYRCPEIVWNP
                     YRRKFSILVRILADGRTTDIPLGRFLREFTCDRPDLFKRSPVNRHAVLKEMAGDPELQ
                     KWREKYLDIYPQEPVPVSRAAPVAREWREIPRTEPDPEITPDNSYRNYL"
BASE COUNT          899 a         1151 c         1082 g         1067 t
ORIGIN      
        1 gtttcactga cacgctcagg ccgcgccggg aatggcgcgg tctgcagaaa atgaggcagg
       61 aaaacgcatg caaagccatg caccttatcg atgcatggct tttttcagta aaaacaggcg
      121 gattttcggg gaattttaca cagactgacg tgatgccagt tgcgcacttt tacgcgaaaa
      181 aatcatgttc tgcgcagggg tgaatttttc acggctgtca tccaccgaag ccgcgtcagg
      241 cctgaatccg atggtcgtta aaatgtcgct atcctgtgcg gaataattaa ttttttcccc
      301 cttcgcaagc caggacagga gggcttcacg cagggcatct gtggcacgct gtatggcaca
      361 gtttcgggca atggccgtca gctcactgta gcccatcagc tccggtgcca gtgccgccgc
      421 cagtgctgtg ccgtgctgct gcataaaatc attcagccgg tcgcggatgc tgatgtgctg
      481 aacggcttca tgcgaacgaa tataacgacc agcggcctga ttcacctgcc attttctgac
      541 ttcgataata ttgcgtaatt cgtccaggcg actgacgttt ctgccttctc ctgacagaag
      601 ccgcagatat tcctgttcgg ccgctgccag ctcatttttg cgttgcagcc atgctgcttt
      661 gttattctga caggtgtcaa aggcctgctg taaggctgtg ctttccatcg ttatctcttt
      721 ctcatcatgc tgaagaataa aaatacggtg tgcggcgacg gccggtgtta accggcagcc
      781 ctcattccag acgcagcgaa tatgattgtg tttttaagcg tactggcggc agttcctgtt
      841 tttcatgcag gctttctgcc agttcgtccg gcgtgaccgg gcggacaatg aaacggttga
      901 tggtctgaag cgttttaaac accagaccac agcccggatc cgtgcacaca taaaaacgct
      961 cggtgacttc ctgagacaga cgccgcgatg ttcttgacag tgcaaggcct ttacatctcc
     1021 gacaacaata tccggtaaca agcattcttt tcggccgttt catactgccg gaggctgacg
     1081 tcagtgaatc gcggtatctc tgtttgcctg aaatgtattc cattcctgaa tctttacagt
     1141 cagagaaaaa gctttcactc gcttcaaatg tcgcagagca ataaatattc cggcactgtg
     1201 caatcattat cttggtgcca tcgtccatga aatgtgcgcg acgggtgtga gcaacatgtc
     1261 cacacgacgg gcagtaaatc atgacagcag ccctctggcc ttaagctctg ctgcctgctg
     1321 gtctattttg tcctgccaca ccttgcgctg tgccggtgtg cctgccacct cataatccat
     1381 gtgcggaagt gttgccgctg acagtccggt cagccggaga accggctcgc cggtgaggct
     1441 gatttgcatc tgtttaattt tctgttccag cgatgatttc acctgctgca tgacagcctt
     1501 ttccggtgcg acgtagccct gatggccggt ggtgttggcg agcggatttt cctgtaccag
     1561 gatactcaga tgcattgccc ggacaagcgc ctcacaggtt tcattcaggg cgtgttccag
     1621 ctcatgctct gcatacagac tcagaaggtg atgatgtgcc ttccggtagg cggtggccgt
     1681 gctgccacac gcccctttca ggcgttcacg ttcgaaattc agcaccacgg ccagattgtc
     1741 atattcctgt accagctccc ggcgtgccac gcgctcaatg tggcgctgtt tcagctcgtc
     1801 gctcaggaca ccaccggctg cacgaaaggc cgtgcgccag tcgtcagcgt tgtttccgtc
     1861 ggcctgcgcc agcgcatttt tttcctgctc tgcccgttca atggccgtga cggtctcatc
     1921 catcaggcgg gcgttctcaa gatgggcggc tctggccttt tccagttgtg ccagcgcggg
     1981 ttgcagatat tcagggatgg tgttgtcaga cattttccgg ctcctcgtca cttcaggtta
     2041 agaaaattgt gacgtacacc ggacaacaac acgacgcatt gcagatgtgc cagccctgac
     2101 acaggagact catcctcaga ccggcaagcc aggaaaaggt cgcaggaaaa accggcttac
     2161 tgtttgtttt tttatatttt actgttcacc tctgttcacc ataagaaaaa agataagtaa
     2221 tacagtaagt taaagggtga acaatcgcag taatgactgt tcaccgtctg ttcaccactg
     2281 ttcacccgtt gatgggcttt ttgtgctgtt tattactttt tatttttatt aattcactga
     2341 aataaataag aaaaaacaat ttgtatttca ctataatttt ttccagctcc ttccagagcg
     2401 ttttaaagct atctggcgct gatgtacaaa aaatacacag ccattgtaag gctgccggaa
     2461 caaattcccc ctgttgcgtc tgccgaaaat attcacaaaa taaagcgcta cccgaagccg
     2521 gacggactta tccggtgctg tatggacatt aacgaggtag cccgatgcaa gctgtttttt
     2581 cttccccgtc tcccgcccca gtgacgccac tgatgccgct gccggacatc acgcaggagc
     2641 gttttttacg tctgccggaa gtgatgcacc tgtgcggcct gtcacgctcg accatctatg
     2701 aacttatccg taagggggaa tttccgccgc aggtgagtct tggcggtaaa aatgtggcct
     2761 ggctgcactc tgaagtcacc gcatggatgg ccgggcgcat tgccggacgc aaacgggggt
     2821 acgacgcatg atgatgcccg ctctgcaaaa actccctttt tctggcttgc ctttttccgg
     2881 catttgcgga tatagttttt ccgctgccgc aaaatcggca gccgggcgtg agaacccgag
     2941 caattcacag gcgacatatg acgcgccacg cgtctttttt tgtgtcgcaa tcaatgccac
     3001 agagcgccag attatggtgt ggtgtgtggt cagtcgtgca gatggcattc cgtgcatcct
     3061 gcccgcgtcc gctcattatg ccgcagagtc aatggtagct caggcggggc agcctccggg
     3121 ctggccggta tcctgtgaag ccggtattct cacccccgtc tgggctatcg ccatcgagcg
     3181 tgagaactcc ggcgatagcg ttatttgcta ttcacaggag gctgccatca tggctacaac
     3241 ccttaccccc tcacaccctg aatttgtctt tgtgtttgcg gctgtccgtc gcgcagaccg
     3301 tcatccccgt atctgtatgc ttcgcaccgt cgccggtgat gaacgcagtg cccgccgttc
     3361 ccttgtccgt gactatgtgc tctcccttgc tgcccgtctg ccggtggtgg aggtgtcccg
     3421 tgcgtaataa aaaagcccct cagaccgtct cagcacgtca tgacgcccgt gaacacctca
     3481 gcattgaggc ttaccataag ctcaaccgcg ccagcgccgt atcccagttt gttgggggtg
     3541 atttgattca ccgtgaactc tccggcctgc atcagctcta cattccgcat attttcagct
     3601 acctgaatga agatattgat tttgtgctga atgagctgaa agccaaaggc ctgtgccgcg
     3661 attttctcgc ccagcagaaa gaccggggag acaggacgca tgtttgattt tccccggccc
     3721 ggtgagattt accgttctgc cggttttccc gatgtggccg tggtcggcat tctggaagac
     3781 ggtattccgt gggaaatgcc gtaccgctgc ccggaaattg tctggaaccc gtaccgccgt
     3841 aaattcagta tccttgtgcg tatcctcgct gacgggcgca ccacagacat cccgctgggg
     3901 cgttttctgc gggaatttac ctgtgaccgt cctgacctgt ttaaacgcag ccccgtaaac
     3961 cggcatgcgg tactgaaaga aatggccgga gacccggaat tacagaaatg gcgggagaaa
     4021 tatctggata tttacccgca ggagcctgtt ccggtcagcc gggcggcacc ggtggcgcgg
     4081 gaatggcggg aaattccccg cacggagcct gacccggaaa tcaccccgga taacagttac
     4141 cgcaattatc tgtaattaaa aaacgacacc cgaaaaaaaa attaaatgtg cgtattcgc
//
LOCUS       DAZKHF020000308         4133 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2201S31241
            SAMN43267800-rid25106413.denovo.310, whole genome shotgun sequence.
ACCESSION   DAZKHF020000308 DAZKHF020000000
VERSION     DAZKHF020000308.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267800
            Sequence Read Archive: SRR30307263
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4133)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4133)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 4133)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350581.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350581.2
            Genome Coverage       :: 151x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:21:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,652
            CDSs (total)                      :: 5,557
            Genes (coding)                    :: 5,320
            CDSs (with protein)               :: 5,320
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 237
            CDSs (without protein)            :: 237
            Pseudo Genes (ambiguous residues) :: 0 of 237
            Pseudo Genes (frameshifted)       :: 102 of 237
            Pseudo Genes (incomplete)         :: 130 of 237
            Pseudo Genes (internal stop)      :: 52 of 237
            Pseudo Genes (multiple problems)  :: 41 of 237
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4133
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267800-rid25106413.denovo.310"
                     /serotype="O 26:H11"
                     /isolate="2201S31241"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-01"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            <1..1538
                     /gene="mltF"
                     /locus_tag="ACHOSC_RS21885"
                     /old_locus_tag="pgaptmp_004377"
     CDS             <1..1538
                     /gene="mltF"
                     /locus_tag="ACHOSC_RS21885"
                     /old_locus_tag="pgaptmp_004377"
                     /EC_number="4.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417053.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008933 - lytic transglycosylase activity
                     [Evidence IEA];
                     GO_process: GO:0000270 - peptidoglycan metabolic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="membrane-bound lytic murein transglycosylase
                     MltF"
                     /protein_id="HGU4550178.1"
                     /translation="YLFIGILALLLAVALWPSIPWFGKADNRIAAIQARGELRVSTIH
                     TPLTYNEINGKPFGLDYELAKQFADYLGVKLKVTVRQNISQLFDDLDNGNADLLAAGL
                     VYNSERVKNYQPGPTYYSVSQQLVYKVGQYRPRTLGNLTAEQLTVAPGHVVVNDLQTL
                     KETKFPELSWKVDDKKGSAELMEDVIEGKLDYTIADSVAISLFQRVHPELAVALDITD
                     EQPVTWFSPLDGDNTLSAALLDFFNEMNEDGTLARIEEKYLGHGDDFDYVDTRTFLRA
                     VDAVLPQLKPLFEKYAEEIDWRLLAAIAYQESHWDAQATSPTGVRGMMMLTKNTAQSL
                     GITDRTDAEQSISGGVRYLQDMMSKVPESVPENERIWFALAAYNMGYAHMLDARALTA
                     KTKGNPDSWADVKQRLPLLSQKPYYSKLTYGYARGHEAYAYVENIRKYQISLVGYLQE
                     KEKQATEAAMQLAQDYPAVSPTELGKEKFPFLSFLSQSSSNYLTHSPSLLFSRKGSEE
                     KQN"
     gene            complement(1535..2038)
                     /gene="tadA"
                     /locus_tag="ACHOSC_RS21890"
                     /old_locus_tag="pgaptmp_004378"
     CDS             complement(1535..2038)
                     /gene="tadA"
                     /locus_tag="ACHOSC_RS21890"
                     /old_locus_tag="pgaptmp_004378"
                     /EC_number="3.5.4.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417054.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008251 - tRNA-specific adenosine
                     deaminase activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0002100 - tRNA wobble adenosine to inosine
                     editing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA adenosine(34) deaminase TadA"
                     /protein_id="HGU4550179.1"
                     /translation="MSEVEFSHEYWMRHALTLAKRAWDEREVPVGAVLVHNNRVIGEG
                     WNRPIGRHDPTAHAEIMALRQGGLVMQNYRLIDATLYVTLEPCVMCAGAMIHSRIGRV
                     VFGARDAKTGAAGSLMDVLHHPGMNHRVEITEGILADECAALLSDFFRMRRQEIKAQK
                     KAQSSTD"
     gene            complement(2096..2731)
                     /gene="yfhb"
                     /locus_tag="ACHOSC_RS21895"
                     /old_locus_tag="pgaptmp_004379"
     CDS             complement(2096..2731)
                     /gene="yfhb"
                     /locus_tag="ACHOSC_RS21895"
                     /old_locus_tag="pgaptmp_004379"
                     /EC_number="3.1.3.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417055.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylglycerophosphatase C"
                     /protein_id="HGU4550180.1"
                     /translation="MATHERRVVFFDLDGTLHQQDMFGSFLRYLLRRQPLNALLVLPL
                     LPIIAIALLIKGRAARWPMSLLLWGCTFGHSEARLQTLQADFVRWFRDNVTAFPLVQE
                     RLTTYLLSSDADIWLITGSPQPLVEAVYFDTPWLPRVNLIASQIQRGYGGWVLTMRCL
                     GHEKVAQLERKIGTPLRLYSGYSDSNQDNPLLYFCQHRWRVTPRGELQQLE"
     gene            2940..3788
                     /locus_tag="ACHOSC_RS21900"
                     /old_locus_tag="pgaptmp_004380"
     CDS             2940..3788
                     /locus_tag="ACHOSC_RS21900"
                     /old_locus_tag="pgaptmp_004380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417056.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HGU4550181.1"
                     /translation="MNGLLRIRQRYQGLAQSDKKLADYLLLQPDTARHLSSQQLANEA
                     GVSQSSVVKFAQKLGYKGFPALKLALSEALASQPESPSVPIHNQIRGDDPLRLVGEKL
                     IKENTAAMYATLNVNSEEKLHECVTMLRSARRIILTGIGASGLVAQNFAWKLMKIGFN
                     AAAVRDMHALLATVQASSPDDLLLAISYTGVRRELNLAADEMLRVGGKVLAITGFTPN
                     ALQQRASHCLYTIAEEQATNSASISACHAQGMLTDLLFIALIQQDLELAPERIRHSEA
                     LVKKLV"
     gene            3844..4104
                     /gene="yfhL"
                     /locus_tag="ACHOSC_RS21905"
                     /old_locus_tag="pgaptmp_004381"
     CDS             3844..4104
                     /gene="yfhL"
                     /locus_tag="ACHOSC_RS21905"
                     /old_locus_tag="pgaptmp_004381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster ferredoxin YfhL"
                     /protein_id="HGU4550182.1"
                     /translation="MALLITKKCINCDMCEPECPNEAISMGDHIYEINSDKCTECVGH
                     YETPTCQKVCPIPNTIVKDPAHVETEEQLWDKFVLMHHADKI"
BASE COUNT         1075 a         1061 c         1034 g          963 t
ORIGIN      
        1 attatctgtt catcggcatt ctggcactgc tgctcgcggt cgctctctgg ccatccattc
       61 cctggtttgg taaagccgac aaccgtatcg ccgccattca agcgcgggga gagttgcgtg
      121 tgagcaccat tcatactccc ctgacttata acgaaatcaa cgggaaacct tttggcctgg
      181 attacgaact ggcgaaacag tttgccgatt acctcggcgt aaaactgaaa gtgaccgtgc
      241 ggcagaatat cagccagctg tttgacgacc ttgataatgg taacgccgac ctgctggcgg
      301 caggactggt ctataacagt gagcgggtaa aaaattatca gcctggccct acctattatt
      361 ccgtgtcaca acaactggtt tataaagtgg gtcagtatcg cccacgtacg ctgggcaacc
      421 tgacggcgga gcaactcacc gttgcaccgg gtcatgtggt ggttaacgat ctccagaccc
      481 tgaaagaaac aaaattcccg gaattaagct ggaaggtaga cgacaaaaaa ggctctgcgg
      541 aattaatgga agatgtcatc gaaggaaaac tcgattacac cattgctgat tctgtcgcca
      601 tcagcctgtt tcagcgcgtt cacccggagc tcgccgtagc gctcgatatc accgatgaac
      661 aaccggtgac ttggtttagc ccgttagatg gcgataatac cctttccgcc gccctgctcg
      721 acttcttcaa cgaaatgaat gaagacggta cgctggcacg cattgaagag aaatacctgg
      781 ggcatggcga tgattttgat tacgtcgata cgcgcacatt tttacgcgcc gtcgatgcgg
      841 tactgccgca gttaaagccc ctgtttgaga aatacgccga agaaattgac tggcgtttgc
      901 tggccgctat tgcttatcag gaatcgcact gggatgcaca ggccacttca ccgacgggtg
      961 tgcgcggcat gatgatgtta accaaaaata ccgcgcaaag cctcggcatt acggatcgta
     1021 ccgatgccga acagagcatc agcggtggcg tgcgttattt gcaggatatg atgagtaaag
     1081 tgccggaaag tgtgccggag aacgagcgga tctggtttgc cctcgctgcg tacaatatgg
     1141 gctatgcgca tatgctggat gcccgcgccc tgacggcaaa aaccaaaggg aatcctgaca
     1201 gttgggctga cgtaaaacag cgtctgcctt tacttagcca gaaaccctat tacagcaagc
     1261 tgacttacgg ctacgctcgt ggacatgaag cctacgctta tgtcgaaaat attcgtaagt
     1321 atcagattag cctggtgggt tatctgcaag agaaagagaa gcaggctaca gaagcggcga
     1381 tgcaactggc gcaggattat ccggcggtat cgcctacgga gttgggcaaa gagaaatttc
     1441 cttttctctc gtttctttcc cagtcgtcat caaactattt gacccattct ccctctctgc
     1501 tgttttccag gaaagggagt gaagagaaac aaaattaatc cgtcgaggat tgcgcttttt
     1561 tctgcgcttt aatttcctgg cggcgcatgc gaaagaagtc actgagcaac gccgcgcact
     1621 catccgccag tattccttcc gtaatttcca ctcggtgatt catacccgga tgatgcagca
     1681 catccattaa agatcccgca gcgccagttt tcgcgtcacg cgcaccaaag accacgcgac
     1741 caatgcgact gtggatcatc gctccggcac acattacaca tggttcaagc gtgacataca
     1801 acgtggcgtc gatcagacga taattttgca tcaccagacc accctgccgc agggccatga
     1861 tttctgcatg tgcggtggga tcatggcgac caatcgggcg gttccagcct tcgccgatta
     1921 cccgattgtt atgcactaat accgcgccga ccggcacttc ccgctcatcc caggcacgtt
     1981 tcgccagcgt cagcgcgtga cgcatccagt attcgtggct aaattcgact tcagacaaaa
     2041 agaaaactcc ggttataaaa gcgcggcgca ttatacacgg acgctatgct ttactctatt
     2101 ccagttgctg gagttcaccg cgcggggtta ctcgccaacg atgctgacag aaataaagca
     2161 gcggattgtc ctgattactg tcgctatagc cactgtacag ccgcagcgga gtgccgattt
     2221 tgcgctccag ttgtgcgacc ttttcatgtc ccagacaacg catcgtcaat acccaaccac
     2281 catagccacg ctgaatttgg ctggcgataa gattaacccg cggcagccag ggcgtatcga
     2341 aataaaccgc ttcaaccagc ggctgcggag agccggtaat caaccagata tcagcatcgg
     2401 aacttaacag gtaggtggtt aatcgctcct gaaccagcgg aaaggcggta acattgtcgc
     2461 gaaaccagcg cacgaaatcg gcctgcaacg tctgtaaacg tgcttcgctg tgaccaaaag
     2521 tgcaccccca cagaagcaga ctcatcggcc agcgtgccgc acgacctttt atcaataacg
     2581 caatggctat aatcggtaac aacggcagga caagtaacgc attcagcggt tggcgacgca
     2641 gtaaatagcg cagaaaactg ccgaacatat cctgctgatg caatgttcca tctaagtcaa
     2701 aaaacaccac acgacgctcg tgagttgcca aaccatactc ctctggactg aaacatcctg
     2761 attaattttc gctactacat agcctaacag atagatcatc acttttccgg caacaatcga
     2821 ccgcatcatg gctaactgga atttttaatt catggcaatt agcggcaatg gaatataaaa
     2881 ttcactcgcg tgtctctcat attgcctgtt gtcgccacta tttcatcgcg aggaaagata
     2941 tgaacggctt acttcgtatc cgtcagcgtt accaggggct tgcccagagt gataaaaaac
     3001 tggcggatta tctgctgcta caacctgata cggcgcgcca tttaagctct cagcaactgg
     3061 ccaacgaagc cggagtcagt cagtccagcg tcgtgaagtt cgcgcaaaaa ctcggctata
     3121 aaggttttcc ggcgcttaag ttggcgttga gtgaagcgct ggcaagccag ccggaatcgc
     3181 cctccgtgcc cattcataac caaatccgcg gtgatgatcc gttacggctg gtcggcgaaa
     3241 aactgattaa agaaaatact gccgctatgt acgcaacgct aaacgttaat agtgaagaga
     3301 aactgcatga atgcgtaaca atgttgcgct ctgcgcggcg gataattctg accggtattg
     3361 gcgcttcggg tctggtggcg caaaactttg cctggaagct gatgaagatt ggcttcaatg
     3421 ctgccgcagt gcgcgatatg catgcgctac tcgcaacagt acaggcgtcg tcccctgacg
     3481 atctgttatt agccatttcc tacaccggtg tacgacgcga gttaaacctg gcggcagatg
     3541 agatgctgcg agtgggcgga aaagtgctgg cgattaccgg ctttactccg aatgccctgc
     3601 aacagcgtgc ttctcattgc ctgtatacca ttgccgaaga acaagcgaca aacagcgctt
     3661 caatctctgc ttgtcacgct cagggaatgt taacggattt gctgttcatt gcgctgattc
     3721 agcaggatct ggaactggca ccagaacgta ttcgtcatag tgaagcgctg gtgaaaaaac
     3781 tggtctgagt aaagaatgcg cgtataatgc ccgcccggtt tgtgttgttt tgagagtttc
     3841 cttatggcgt tgttaatcac taaaaaatgc atcaattgtg atatgtgtga acccgaatgc
     3901 ccgaatgagg cgatttcaat gggagatcat atctacgaga ttaacagcga taagtgtacc
     3961 gaatgcgtag ggcactacga gacaccaacc tgccagaagg tgtgcccgat ccccaatact
     4021 attgtgaaag atccggcgca tgtcgagaca gaagaacagt tgtgggataa atttgtgctg
     4081 atgcaccacg cggataaaat ttaatattct actctggaag tagagtatta att
//
LOCUS       DAZKHF020000309         4126 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2201S31241
            SAMN43267800-rid25106413.denovo.311, whole genome shotgun sequence.
ACCESSION   DAZKHF020000309 DAZKHF020000000
VERSION     DAZKHF020000309.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267800
            Sequence Read Archive: SRR30307263
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4126)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4126)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 4126)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350581.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350581.2
            Genome Coverage       :: 151x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:21:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,652
            CDSs (total)                      :: 5,557
            Genes (coding)                    :: 5,320
            CDSs (with protein)               :: 5,320
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 237
            CDSs (without protein)            :: 237
            Pseudo Genes (ambiguous residues) :: 0 of 237
            Pseudo Genes (frameshifted)       :: 102 of 237
            Pseudo Genes (incomplete)         :: 130 of 237
            Pseudo Genes (internal stop)      :: 52 of 237
            Pseudo Genes (multiple problems)  :: 41 of 237
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4126
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267800-rid25106413.denovo.311"
                     /serotype="O 26:H11"
                     /isolate="2201S31241"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-01"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            56..1309
                     /gene="ynfM"
                     /locus_tag="ACHOSC_RS21910"
                     /old_locus_tag="pgaptmp_004382"
     CDS             56..1309
                     /gene="ynfM"
                     /locus_tag="ACHOSC_RS21910"
                     /old_locus_tag="pgaptmp_004382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HGU4550183.1"
                     /translation="MSRTTTVDGAPASDTDKQSISQPNQFIKRGTPQFMRVTLALFSA
                     GLATFALLYCVQPILPVLSQEFGLTPANSSISLSISTAMLAIGLLFTGPLSDAIGRKP
                     VMVTALLLASICTLLSTMMTSWHGILIMRALIGLSLSGVAAVGMTYLSEEIHPSFVAF
                     SMGLYISGNSIGGMSGRLISGVFTDFFNWRIALAAIGCFALASALMFWKILPESRHFR
                     PTSLRPKTLFINFRLHWRDRGLPLLFAEGFLLMGSFVTLFNYIGYRLMLSPWHVSQAV
                     VGLLSLAYLTGTWSSPKAGTMTTRYGRGPVMLFSTGVMLFGLLMTLFSSLWLIFAGML
                     LFSAGFFAAHSVASSWIGPRAKRAKGQASSLYLFSYYLGSSIAGTLGGVFWHNYGWNG
                     VGAFIALMLVIALLVGTRLHRRLHA"
     gene            complement(1358..1477)
                     /locus_tag="ACHOSC_RS21915"
                     /old_locus_tag="pgaptmp_004383"
     CDS             complement(1358..1477)
                     /locus_tag="ACHOSC_RS21915"
                     /old_locus_tag="pgaptmp_004383"
                     /inference="COORDINATES: protein motif:HMM:NF033695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KPN_01571 family protein"
                     /protein_id="HGU4550184.1"
                     /translation="MTHSKMNPFIWVLIILMTLDALRELAGASSILGWLLTLV"
     gene            1733..2041
                     /gene="asr"
                     /locus_tag="ACHOSC_RS21920"
                     /old_locus_tag="pgaptmp_004384"
     CDS             1733..2041
                     /gene="asr"
                     /locus_tag="ACHOSC_RS21920"
                     /old_locus_tag="pgaptmp_004384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310330.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid resistance repetitive basic protein Asr"
                     /protein_id="HGU4550185.1"
                     /translation="MKKVLALVVAAAMGLSSAAFAAETATTPAPTATTTKAAPAKTTH
                     HKKQHKAAPAQKAQAAKKHHKNTKAEQKAPEQKAQAAKKHAGKHSHQQPAKPAAQPAA
                     "
     gene            2134..2217
                     /locus_tag="ACHOSC_RS21925"
                     /old_locus_tag="pgaptmp_004385"
     CDS             2134..2217
                     /locus_tag="ACHOSC_RS21925"
                     /old_locus_tag="pgaptmp_004385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4550186.1"
                     /translation="MVGRYRFEFILIILILCALIAARFYLS"
     gene            2317..3138
                     /gene="ydgD"
                     /locus_tag="ACHOSC_RS21930"
                     /old_locus_tag="pgaptmp_004386"
     CDS             2317..3138
                     /gene="ydgD"
                     /locus_tag="ACHOSC_RS21930"
                     /old_locus_tag="pgaptmp_004386"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine protease"
                     /protein_id="HGU4550187.1"
                     /translation="MRTTIAVVLGAISLTSAFVFADKPDVAKSANDEVSTLFFGHDDR
                     VPVNDTTQSPWDAVGQLETASGNLCTATLIAPNLALTAGHCLLTPPKGKADKAVALRF
                     VSNKGLWRYDIHDIEGRVDPTLGKRLKADGDGWIVPPAAAPWDFGLIVLRNPPSGITP
                     LPLFEGDKAALTAALKSAGRKVTQAGYPEDHLDTLYSHQNCEVTGWAQTSVMSHQCDT
                     LPGDSGSPLMLHTDDGWQLIGVQSSAPAAKDRWRADNRAISVTGFRDKLDQLSQK"
     gene            complement(3177..3506)
                     /gene="mdtI"
                     /locus_tag="ACHOSC_RS21935"
                     /old_locus_tag="pgaptmp_004387"
     CDS             complement(3177..3506)
                     /gene="mdtI"
                     /locus_tag="ACHOSC_RS21935"
                     /old_locus_tag="pgaptmp_004387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug/spermidine efflux SMR transporter
                     subunit MdtI"
                     /protein_id="HGU4550188.1"
                     /translation="MAQFEWVHAAWLALAIVLEIVANVFLKFSDGFRRKIFGLLSLAA
                     VLAAFSALSQAVKGIDLSVAYALWGGFGIAATLAAGWILFGQRLNRKGWIGLVLLLAG
                     MIMVKLA"
     gene            complement(3493..3858)
                     /gene="mdtJ"
                     /locus_tag="ACHOSC_RS21940"
                     /old_locus_tag="pgaptmp_004388"
     CDS             complement(3493..3858)
                     /gene="mdtJ"
                     /locus_tag="ACHOSC_RS21940"
                     /old_locus_tag="pgaptmp_004388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015606 - spermidine transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015848 - spermidine transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug/spermidine efflux SMR transporter
                     subunit MdtJ"
                     /protein_id="HGU4550189.1"
                     /translation="MYIYWILLGLAIATEITGTLSMKWASVSEGNGGFILMLVMISLS
                     YIFLSFAVKKIALGVAYALWEGIGILFITLFSVLLFDESLSLMKIAGLTTLVAGIVLI
                     KSGTRKARKPELEVNHGAV"
BASE COUNT         1032 a         1036 c          992 g         1066 t
ORIGIN      
        1 ttttcctaac ctatgtataa gcctgatcca caggcatatt tagcaaggat ttcaagtgag
       61 ccgtactaca actgttgatg gcgctccggc aagcgacact gacaagcaaa gcatttctca
      121 gccaaatcaa tttattaaac gcggtacgcc gcaatttatg cgcgtcaccc tggcgctgtt
      181 ctctgccgga ctggcaacat ttgcacttct ctattgtgtg cagcctatcc ttccggtgct
      241 ttcgcaggag tttggcttaa cgcccgcgaa cagtagtatt tcactgtcca tttccacggc
      301 gatgttggct attggtttgc tgtttactgg ccccctatcc gatgccattg gtcgcaaacc
      361 agtgatggtc acggcgctac tgttggcctc catttgtacg ttactttcga caatgatgac
      421 cagctggcac ggcattttga ttatgcgcgc cttgattggg ctttcgttaa gtggcgtggc
      481 agctgttggc atgacttatc ttagcgagga aatccacccc agtttcgtgg ccttttcgat
      541 ggggttgtat atcagcggca actcaattgg cggcatgagc ggacgcttaa ttagtggtgt
      601 cttcacggac tttttcaact ggcgaattgc tctggcggca atcggttgtt tcgcgctagc
      661 ctcggcgttg atgttctgga aaatcctccc tgaatcacgc cattttcgcc cgacttcgct
      721 gcgacctaaa acgttgttta tcaactttcg tctgcactgg cgtgaccggg gattaccgtt
      781 attgttcgca gaaggctttt tgctgatggg gtcgttcgtc acgctgttta attacatcgg
      841 atatcggttg atgctctccc cctggcatgt cagtcaggcc gtggttggct tattatcgct
      901 ggcttatttg accggtacat ggagctcacc caaagccgga accatgacca cccgctacgg
      961 gcgcggtccg gtgatgttgt tttcgacggg ggttatgctg tttggtttac tgatgacctt
     1021 attcagctcg ctgtggctga tctttgccgg aatgttactc ttctcagcag gattcttcgc
     1081 agcccactca gttgccagca gctggattgg cccccgcgca aaacgcgcta aaggccaggc
     1141 ctcctcgctg tatctgttca gttactatct ggggtcgagt attgccggga cgctgggtgg
     1201 tgttttctgg cataactatg gctggaacgg cgtcggcgca tttattgctc tgatgctggt
     1261 cattgctctg ctggtcggga cgcgtttgca tcgtcgtctg cacgcctgaa aaataagccc
     1321 ggactgcggt aactacacgt ccggacttat tgcttgctca aaccaacgtt aatagccatc
     1381 ctaaaataga cgaagcgcca gccaattccc gcagcgcgtc tagcgtcatc aggattataa
     1441 gtacccaaat aaacggattc attttgctgt gtgtcattta ttactgatgc gcagttattc
     1501 taccgctttg taagtagtaa aatagttaac ccgatcaaga ctactattat tggtagctaa
     1561 atttccctta agtcacaata cgttattatc aacgctgtaa tttattcagc gtttgtacat
     1621 atcgttacac gctgaaacca accactcacg gaagtctgcc attcccaggg atatagttat
     1681 ttcaacggcc ccgcagtggg gttaaatgaa aaaacaaatt gagggtatga caatgaaaaa
     1741 agtattagct ctggttgttg ccgctgctat gggtctgtct tctgccgcct ttgctgcaga
     1801 gactgcgacc acacctgctc cgactgcgac gaccaccaaa gcagcgccgg cgaaaacgac
     1861 acatcataaa aaacagcata aagcagcacc tgcccagaaa gcgcaggcgg ctaaaaagca
     1921 tcataaaaat acgaaagctg aacagaaagc ccctgaacaa aaagcgcagg cagcgaagaa
     1981 acacgccggg aaacacagcc atcagcaacc ggcaaaacct gctgcacaac ccgcagcgta
     2041 agttttcaac agtaatgctg gcgcgccccc tcgcgcctga aaattacggt gctaagcagg
     2101 taacgtttag caccgccttt agcggagggt agtatggtgg gccgttatcg ctttgagttc
     2161 attcttatca tccttatttt atgcgcactg attgccgccc gtttttatct ttcctgactg
     2221 tagttatctg attttactcc cactttcatc ccgtcccgtc tatagtattt atgagggttt
     2281 gcttttaata atcataatta cccaccagag tgtgatatgc gtacaaccat tgctgtagtg
     2341 ttgggtgcaa ttagtttgac gtctgctttt gtgtttgcag ataaaccaga cgttgccaaa
     2401 tcggcaaacg atgaggtcag caccctgttt tttggtcatg atgatcgtgt gccagtgaat
     2461 gacacgaccc aatcaccgtg ggatgcggtt gggcaactgg aaacggccag cggcaattta
     2521 tgtacggcga cgctgattgc acccaatctg gcattaacgg caggacactg tttattgacg
     2581 ccgcctaagg gtaaagcgga taaggcggta gcgctgcgtt ttgtgtcaaa taaaggtctt
     2641 tggcgctatg acatccacga catagaaggc cgcgttgatc cgacactggg aaagcggtta
     2701 aaagcagatg gggatggttg gattgttcct cccgcagctg caccatggga cttcggcttg
     2761 attgtgctgc gtaatccccc ttctggcatt acaccgttgc cgttatttga gggagataaa
     2821 gccgcgctta ctgccgcatt aaaatcggct ggtcgtaaag tgactcaggc gggatatcct
     2881 gaagatcatc tcgatacgtt atacagtcat caaaactgtg aagtgactgg ctgggcgcaa
     2941 acgtcggtga tgtcacatca gtgcgatacc ttgccgggtg acagcggttc gcctttgatg
     3001 ttgcataccg atgacggctg gcaattaatt ggggtgcaaa gttcggctcc tgccgcgaaa
     3061 gatcgctggc gcgccgataa ccgggccatt tctgttaccg gttttcgcga caagctggat
     3121 caactgtcgc aaaaataatg ttcaaacgct gcccgacagt gcgggcagcg tgttcatcag
     3181 gcaagtttca ccatgatcat tccagccaac agcaagacca ggccaatcca gcctttacga
     3241 tttaaccgtt gaccaaacaa gatccaacct gcggctaacg tggcggcaat accaaacccg
     3301 ccccacaatg cataagcgac agacaagtcg atccctttta cggcctgaga aagcgcacta
     3361 aaggcagcca gcaccgccgc cagggagagc aagccaaata ttttgcgacg aaagccgtca
     3421 gaaaatttca aaaagacgtt agcaacgatt tccagcacga ttgccaatgc cagccaggcg
     3481 gcgtgaaccc attcaaactg cgccatggtt cacttccagt tcaggtttac gcgctttacg
     3541 ggtacctgat ttgatcaaca caatcccggc gaccagggtg gttaacccgg caattttcat
     3601 cagcgataaa ctttcgtcga ataacaaaac gctaaacaag gtaataaata aaataccgat
     3661 accttcccac agcgcataag ctacgcctaa ggcgatcttt ttaacggcga aagagagaaa
     3721 tatatacgac agagaaatca tcaccagcat taaaataaag ccgccatttc cctcactgac
     3781 gctcgcccat ttcattgaca gcgtaccggt aatttctgta gcaatagcca ggcctaataa
     3841 aatccaataa atatacattg tccttctcct gcaagagaat tattttaatt ttcgcttaat
     3901 tcagcgaaac caaagtaaag tggtcaagcc ccgcgcatag ggcaagctta ggcagaagaa
     3961 aggactaaag cgcgttgcgc cagtgctgct cacctacgag caagatagtg gatgaggtac
     4021 gaagatgggt aaatgttgaa aataacgtcc tgaacaaatt gtccataata ttacaattat
     4081 ccgcagtgtt gcttctcgtc atcgcggatg ataattgtcc tcggta
//
LOCUS       DAZKHF020000310         4060 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2201S31241
            SAMN43267800-rid25106413.denovo.312, whole genome shotgun sequence.
ACCESSION   DAZKHF020000310 DAZKHF020000000
VERSION     DAZKHF020000310.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267800
            Sequence Read Archive: SRR30307263
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4060)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4060)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 4060)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350581.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350581.2
            Genome Coverage       :: 151x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:21:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,652
            CDSs (total)                      :: 5,557
            Genes (coding)                    :: 5,320
            CDSs (with protein)               :: 5,320
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 237
            CDSs (without protein)            :: 237
            Pseudo Genes (ambiguous residues) :: 0 of 237
            Pseudo Genes (frameshifted)       :: 102 of 237
            Pseudo Genes (incomplete)         :: 130 of 237
            Pseudo Genes (internal stop)      :: 52 of 237
            Pseudo Genes (multiple problems)  :: 41 of 237
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4060
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267800-rid25106413.denovo.312"
                     /serotype="O 26:H11"
                     /isolate="2201S31241"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-01"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            <1..1820
                     /locus_tag="ACHOSC_RS21945"
                     /old_locus_tag="pgaptmp_004389"
     CDS             <1..1820
                     /locus_tag="ACHOSC_RS21945"
                     /old_locus_tag="pgaptmp_004389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="phage terminase large subunit family protein"
                     /protein_id="HGU4550190.1"
                     /translation="NYYLPKESAYQEGRWETLPFQRAIMNAMGSDYIREVNVVKSARV
                     GYSKMLLGVYAYFIEHKQRNTLIWLPTDGDAENFMKTHVEPTIRDIPSLLALAPWYGK
                     KHRDNTLTMKRFTNGRGFWCLGGKAAKNYREKSVDVAGYDELAAFDDDIEQEGSPTFL
                     GDKRIEGSVWPKSIRGSTPKVRGTCQIERAASESPHFMRFHVACPHCGEEQYLKFGDK
                     ETPFGLKWTPDDPSSVFYLCEHNACVIRQQELDFTDARYICEKTGIWTRDGILWFSSS
                     GEEIEPPDSVTFHIWTAYSPFTTWVQIVKDWMKTKGDTGKRKTFVNTTLGETWEAKIG
                     ERPDAEVMAERKEHYSAPVPDRVAYLTAGIDSQLDRYEMRVWGWGPGEESWLIDRQII
                     MGRHDDEQTLLRVDEAINKTYTRRNGAEMSVSRICWDIGGIDPTIVYERSKKHGLFRV
                     IPIKGASVYGKPVASMPRKRNKNGVYLTEIGTDTAKEQIYNRFTLTPEGDEPLPGAVH
                     FPNNPDIFDLTEAQQLTAEEQVEKWVDGRKKILWDSKKRRNEALDCFVYALAALRISI
                     SRWQLDLSALLASLQEEDGAATNKKTLADYARALSGEDE"
     gene            1817..2023
                     /locus_tag="ACHOSC_RS21950"
                     /old_locus_tag="pgaptmp_004390"
     CDS             1817..2023
                     /locus_tag="ACHOSC_RS21950"
                     /old_locus_tag="pgaptmp_004390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0019058 - viral life cycle [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage head-tail joining protein"
                     /protein_id="HGU4550191.1"
                     /translation="MTRQEELAAARAALHDLMTGKRVATVQKDGRRVEFTATSVSDLK
                     KYIAELEVQTGMTQRRRGPAGFYV"
     gene            2020..3621
                     /locus_tag="ACHOSC_RS21955"
                     /old_locus_tag="pgaptmp_004391"
     CDS             2020..3621
                     /locus_tag="ACHOSC_RS21955"
                     /old_locus_tag="pgaptmp_004391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HGU4550192.1"
                     /translation="MKTPTIPTLLGPDGMTSLREYAGYHGGGSGFGGQLRAWNPPSES
                     VDAALLPNFTRGNARADDLVRNNGYAANAIQLHQDHIVGSFFRLSHRPSWRYLGIGEE
                     EARAFSREVEAAWKEFAEDDCCCIDVERKRTFTMMIREGVAMHAFNGELFVQATWDTS
                     SSRLFRTQFRMVSPKRISNPNNTGDSRNCRAGVQINDSGAALGYYVSEDGYPGWMPQK
                     WTWIPRELPGGRASFIHVFEPVEDGQTRGANVFYSVMEQMKMLDTLQNTQLQSAIVKA
                     MYAATIESELDTQSAMDFILGANSNEQRDKLAGWIGEIAAYYAAAPVRLGGAKVPHLM
                     PGDSLNLQTAQDTDNGYSVFEQSLLRYIAAGLGVSYEQLSRNYAQMSYSTARASANES
                     WAYFMGRRKFVASRQASQMFLCWLEEAIVRRVVTLPSKARFSFQEARSAWGNCDWIGS
                     GRMAIDGLKEVQEAVMLIEAGLSTYEKECAKRGDDYQEIFAQQVRETMERRAAGLKPP
                     AWAAAAFESGLRQSTEEEKSDSRAA"
     gene            3602..>4060
                     /locus_tag="ACHOSC_RS21960"
                     /old_locus_tag="pgaptmp_004392"
     CDS             3602..>4060
                     /locus_tag="ACHOSC_RS21960"
                     /old_locus_tag="pgaptmp_004392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S49 family peptidase"
                     /protein_id="HGU4550193.1"
                     /translation="MTAELRNLPHIASMAFNEPLMLEPAYARVFFCALAGQLGISRLT
                     DAVSGDSLTAQEALATLALSGDDDGPRQARSYQVMNGIAVLPVSGTLVSRTRALQPYS
                     GMTGYNGIIARLQQAASDPMVDGILLDMDTPGGMVAGAFDCADIIARVRDI"
BASE COUNT          926 a         1053 c         1253 g          828 t
ORIGIN      
        1 ccaattacta tctcccgaaa gaatccgcat accaggaagg gcgctgggaa acactgccct
       61 ttcagcgggc catcatgaat gcgatgggca gcgactacat ccgcgaggtg aatgtggtga
      121 agtctgcccg tgttggttat tccaaaatgc tgttgggtgt ttatgcctac ttcatagagc
      181 ataagcagcg caacaccctt atctggttgc cgacggatgg cgatgccgag aactttatga
      241 aaacccacgt cgagccgacc atccgtgata ttccatcgct gctggcgctg gccccgtggt
      301 atggcaaaaa gcaccgggat aacacgctca ccatgaagcg tttcaccaat gggcgtggtt
      361 tctggtgcct gggcggtaaa gcggcaaaaa actaccgtga aaagtcagtg gatgtggcgg
      421 gttatgatga acttgctgcc tttgatgatg atattgaaca ggaaggctct ccgacgttcc
      481 tgggtgacaa gcgtattgaa ggctcggtct ggccaaagtc catccgtggc tccacgccca
      541 aagtgagagg cacctgtcag attgagcgtg cagccagtga atccccgcat tttatgcgtt
      601 ttcatgttgc ctgcccgcac tgcggggagg agcagtacct taaatttggc gataaagaga
      661 cgccgtttgg cctcaaatgg acgccggatg atccctccag cgtgttttat ctctgcgaac
      721 ataatgcctg cgtcatccgc cagcaggagc tggacttcac tgatgcccgt tatatctgcg
      781 aaaagaccgg gatctggacc cgtgatggca ttctctggtt ttcgtcatcc ggtgaagaga
      841 ttgagccgcc ggacagtgtg acctttcaca tctggacggc gtacagcccg ttcaccacct
      901 gggtgcagat tgtcaaagac tggatgaaaa cgaaagggga tacgggaaaa cgtaaaacct
      961 tcgtgaacac cacgctcggt gagacgtggg aggcgaaaat tggcgaacgt ccggatgctg
     1021 aagtgatggc agagcggaaa gagcattatt cagcgcccgt tcctgaccgt gtggcttacc
     1081 tgaccgccgg tatcgactcc cagctggacc gctacgaaat gcgcgtatgg ggatgggggc
     1141 cgggtgagga aagctggctg attgaccggc agattattat gggccgccac gacgacgagc
     1201 agacgctgct gcgtgtggat gaggccatca ataaaaccta tacccgccgg aatggtgcag
     1261 aaatgtcggt atcccgtatc tgctgggata ttggcgggat tgacccgacc attgtgtatg
     1321 aacgctcgaa aaagcatggg ctgttccggg tgatccccat taaaggggca tccgtctacg
     1381 gaaagccggt ggccagcatg ccacgtaagc gaaacaaaaa cggggtttac cttaccgaaa
     1441 ttggtacgga taccgcgaaa gagcagattt ataaccgctt cacactgacg ccggaagggg
     1501 atgaaccgct tcccggtgcc gttcacttcc cgaataaccc ggatattttt gatctgaccg
     1561 aagcgcagca gctgacagct gaagagcagg tcgaaaaatg ggtggatggc aggaaaaaaa
     1621 tactgtggga cagcaaaaaa cgacgcaatg aggctctcga ctgcttcgtt tatgcgctgg
     1681 cggcgctgcg catcagtatt tcccgctggc agctggatct cagtgcgctg ctggcgagcc
     1741 tgcaggaaga ggatggtgca gcaaccaaca agaaaacact ggcagattac gcccgtgcct
     1801 tatccggaga ggatgaatga cgcgacagga agaacttgcc gctgcccgtg cggcactgca
     1861 tgacctgatg acaggtaaac gggtggcaac agtacagaaa gacggacgaa gggtggagtt
     1921 tacggccact tccgtgtctg acctgaaaaa atatattgca gagctggaag tgcagaccgg
     1981 catgacacag cgacgcaggg gacctgcagg attttatgta tgaaaacgcc caccattccc
     2041 acccttctgg ggccggacgg catgacatcg ctgcgcgaat atgctggtta tcacggcggt
     2101 ggcagcggat ttggtgggca gttgcgggcg tggaacccac cgagtgaaag tgtggatgca
     2161 gccctgctgc ccaactttac ccgtggcaat gcccgcgcag acgatctggt acgcaataac
     2221 ggctatgccg ccaacgccat ccagctgcat caggatcata tcgtcgggtc ttttttccgg
     2281 ctcagtcatc gcccaagctg gcgctatctg ggcatcgggg aggaagaagc ccgtgccttt
     2341 tcccgcgagg ttgaagcggc atggaaagaa tttgccgagg atgactgctg ctgcattgac
     2401 gttgagcgaa aacgcacgtt taccatgatg attcgggaag gtgtggccat gcacgccttt
     2461 aacggagaac tgttcgttca ggccacctgg gataccagtt cgtcgcggct tttccggaca
     2521 cagttccgga tggtcagccc gaagcgcatc agcaatccga acaataccgg cgacagccgg
     2581 aactgccgtg ccggtgtgca gattaatgac agcggtgcgg cgctgggata ttacgtcagc
     2641 gaggacgggt atcctggctg gatgccgcag aaatggacat ggataccccg tgagttaccc
     2701 ggcgggcgcg cctcgttcat tcacgtcttt gaacccgtgg aggacgggca gacccgcggt
     2761 gcaaatgtgt tttacagcgt gatggagcag atgaagatgc tcgacacgct gcagaacacg
     2821 cagctgcaga gcgccattgt gaaggcgatg tatgccgcca ccattgaaag tgagctggat
     2881 acgcagtcag cgatggattt tattcttggc gcgaacagta atgagcagcg ggacaagctg
     2941 gcgggctgga ttggtgaaat tgccgcgtat tacgccgccg cgccggtccg gctgggaggc
     3001 gcaaaagtgc cgcacctgat gccgggtgac tctctgaacc tgcagacggc tcaggacacg
     3061 gataacggct actccgtgtt tgagcagtca ctgctgcggt atatcgctgc cggactgggt
     3121 gtctcgtatg agcagctttc ccggaattac gcccagatga gctactccac ggcacgggcc
     3181 agtgcgaacg agtcgtgggc gtactttatg gggcggcgaa aattcgtcgc atcccgtcag
     3241 gcgagccaga tgtttctgtg ctggctggaa gaggccatcg ttcgccgcgt ggtgacgtta
     3301 ccttcaaaag cgcgtttcag ctttcaggaa gcccgcagcg cctgggggaa ctgtgactgg
     3361 ataggctccg gtcgtatggc catcgatggt ctgaaagaag tacaggaagc ggtgatgctg
     3421 atagaagccg gactgagtac ctacgagaaa gagtgcgcaa aacgcggtga cgactatcag
     3481 gaaatttttg cccagcaggt ccgtgaaacg atggagcgcc gcgcagccgg tcttaaaccg
     3541 cccgcctggg cggctgcggc atttgaatcc ggactgcgac aatcaacaga ggaggagaag
     3601 agtgacagca gagctgcgta atctcccgca tattgccagc atggctttta atgagccgct
     3661 gatgcttgaa cccgcctatg cgcgggtttt cttttgtgcg cttgcaggcc agcttgggat
     3721 cagccgcctg acggatgcgg tgtccggcga cagcctgact gcccaggagg cactcgcgac
     3781 gctggcatta tccggtgatg atgacggacc acgacaggcc cgcagttatc aggtcatgaa
     3841 cggcatcgcc gtgctgccgg tgtccggcac gctggtcagc cggacgcggg cgctgcagcc
     3901 gtactcgggg atgaccggtt acaacggcat tatcgcccgt ctgcaacagg ctgccagcga
     3961 tccgatggtg gacggcattc tgctcgatat ggacacgccc ggcgggatgg tggcgggggc
     4021 atttgactgc gctgacatca tcgcccgtgt gcgtgacata
//
LOCUS       DAZKHF020000311         4016 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2201S31241
            SAMN43267800-rid25106413.denovo.313, whole genome shotgun sequence.
ACCESSION   DAZKHF020000311 DAZKHF020000000
VERSION     DAZKHF020000311.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267800
            Sequence Read Archive: SRR30307263
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4016)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4016)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 4016)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350581.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350581.2
            Genome Coverage       :: 151x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:21:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,652
            CDSs (total)                      :: 5,557
            Genes (coding)                    :: 5,320
            CDSs (with protein)               :: 5,320
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 237
            CDSs (without protein)            :: 237
            Pseudo Genes (ambiguous residues) :: 0 of 237
            Pseudo Genes (frameshifted)       :: 102 of 237
            Pseudo Genes (incomplete)         :: 130 of 237
            Pseudo Genes (internal stop)      :: 52 of 237
            Pseudo Genes (multiple problems)  :: 41 of 237
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4016
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267800-rid25106413.denovo.313"
                     /serotype="O 26:H11"
                     /isolate="2201S31241"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-01"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            complement(99..1778)
                     /gene="bcsG"
                     /locus_tag="ACHOSC_RS21965"
                     /old_locus_tag="pgaptmp_004393"
     CDS             complement(99..1778)
                     /gene="bcsG"
                     /locus_tag="ACHOSC_RS21965"
                     /old_locus_tag="pgaptmp_004393"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0030244 - cellulose biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose biosynthesis protein BcsG"
                     /protein_id="HGU4550194.1"
                     /translation="MTQFTQNTAMPSSLWQYWRGLSGWNFYFLVKFGLLWAGYLNFHP
                     LLNLVFAAFLLMPIPRYSLHRLRHWIALPIGFALFWHDTWLPGPESIMSQGSQVAGFS
                     TDYLIDLVTRFINWQMIGAIFVLLVAWLFLSQWIRITVFVVAILLWLNVLTLAGPSFS
                     LWPAGQPTTTVTTTGGNAAATVAATGGAPVVSDMPAQTAPPTTANLNAWLNNFYNAEA
                     KRKSTFPSSLPADAQPFELLVINICSLSWSDIEAAGLMSHPLWSHFDIEFKNFNSATS
                     YSGPAAIRLLRASCGQTSHTNLYQPANNDCYLFDNLSKLGFTQHLMMGHNGQFGGFLK
                     EVRENGGMQTELMDQTNLPVILLGFDGSPVYDDTAVLNRWLDVTEKDKNSRSATFYNT
                     LPLHDGNHYPGVSKTADYKARAQKFFDELDAFFTELEKSGRKVMVVVVPEHGGALKGD
                     RMQVSGLRDIPSPSITDVPVGVKFFGMKAPHQGAPIVIDQPSSFLAISDLVVRVLDGK
                     IFTEDNVDWKKLTSGLPQTAPVSENSNAVVIQYQDKPYVRLNGGDWVPYPQ"
     gene            complement(1775..1966)
                     /gene="bcsF"
                     /locus_tag="ACHOSC_RS21970"
                     /old_locus_tag="pgaptmp_004394"
     CDS             complement(1775..1966)
                     /gene="bcsF"
                     /locus_tag="ACHOSC_RS21970"
                     /old_locus_tag="pgaptmp_004394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417994.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0052324 - plant-type cell wall cellulose
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose biosynthesis protein BcsF"
                     /protein_id="HGU4550195.1"
                     /translation="MMTISDIIEIIVVCALIFFPLGYLARHSLRRIRDTLRLFFAKPR
                     YVKPAGTLRRTEKARATKK"
     gene            complement(1963..3534)
                     /gene="bcsE"
                     /locus_tag="ACHOSC_RS21975"
                     /old_locus_tag="pgaptmp_004395"
     CDS             complement(1963..3534)
                     /gene="bcsE"
                     /locus_tag="ACHOSC_RS21975"
                     /old_locus_tag="pgaptmp_004395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0035438 - cyclic-di-GMP binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose biosynthesis c-di-GMP-binding protein
                     BcsE"
                     /protein_id="HGU4550196.1"
                     /translation="MRDIVDPVFSIGISSLWDELRHMPAGGVWWFNVDRHEDAISLAN
                     QTIASQAETAHVAVISMDSDPAKIFQLDDSQGPEKIKLFSMLNHEKGLYYLTRDLQCS
                     IDPHNYLFILVCANNAWQNIPAERLRSWLDKMNKWSRLNHCSLLVINPGNNNDKQFSL
                     LLEEYRSLFGLASLRFQGDQHLLDIAFWCNEKGVSARQQLSVQQQNGIWTLVQSEEAE
                     IQPRSDEKRILSNVAVLEGAPPLSEHWQLFNNNEVLFNEARTAQAATVVFSLQQNAQI
                     EPLARSIHTLRRQRGSAMKILVRENTASLRATDERLLLACGANMVIPWNAPLSRCLTM
                     IESVQGQKFSRYVPEDITTLLSMTQPLKLRGFQKWDVFCNAVNNMMNNPLLPAHGKGV
                     LVALRPVPGIRVEQALTLCRPNRTGDIMTIGGNRLVLFLSFCRINDLDTALNHIFPLP
                     TGDIFSNRMVWFEDDQISAELVQMRLLAPEQWGMPLPLTQSSKPVINAEHDGRHWRRI
                     PEPMRLLDDAVERSS"
     gene            3807..3995
                     /gene="bcsR"
                     /locus_tag="ACHOSC_RS21980"
                     /old_locus_tag="pgaptmp_004396"
     CDS             3807..3995
                     /gene="bcsR"
                     /locus_tag="ACHOSC_RS21980"
                     /old_locus_tag="pgaptmp_004396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose biosynthesis protein BcsR"
                     /protein_id="HGU4550197.1"
                     /translation="MNNNEPDTLPDPAIGYIFQNDIVALKQAFSLPDIDYADISQREQ
                     LAAALKRWPLLAEFAQQK"
BASE COUNT         1051 a          984 c         1008 g          973 t
ORIGIN      
        1 aagcggaagt aacgtgtcat gcgggcgtca ggctgccgta atggcaattt gcgcccggat
       61 caggccgcag aggttaaact ctgcggcctt tttcgtgctt actgcgggta aggcacccag
      121 tcgccgccgt tcaggcgaac gtacggttta tcctggtatt gaataactac tgcatttgag
      181 ttctcggaga ccggtgctgt ttgtggcaac ccactggtga gttttttcca gtcaacattg
      241 tcttcggtga aaatcttgcc atcaagaacg cgaaccacca gatcggagat agccaggaag
      301 ctgctcggtt ggtcaatgac aatcggtgcc ccctgatgtg gtgccttcat gccgaagaat
      361 ttcaccccaa cggggacgtc tgtgatagac gggctaggga tatcacgtag gccagatacc
      421 tgcattctgt cgcccttcag cgcgccgccg tgttccggca ccacgaccac catcacttta
      481 cgacccgatt tctccagttc agtaaagaag gcgtccagtt catcaaagaa tttctgcgcc
      541 cgcgctttgt aatccgctgt tttgctgacc cccggataat ggttgccgtc atgcagtgga
      601 agcgtgttgt agaacgtggc actacggcta tttttatctt tttcggtaac gtccagccag
      661 cggttaagca cggcggtatc gtcataaacc ggcgaaccat caaagcccag caaaataacc
      721 ggcagatttg tttgatccat caattcagtc tgcatgccgc cattttcgcg aacttctttc
      781 aaaaaaccgc cgaactggcc gttatgcccc atcatcaggt gctgggtaaa gcccagtttc
      841 gaaaggttat caaacagata gcagtcgtta tttgccggtt gatacagatt agtgtgcgaa
      901 gtctgcccgc agctggcgcg cagtaaacgg atcgccgccg ggccactgta ggaggtggcg
      961 gagttaaagt tcttgaactc aatatcgaaa tgcgaccaca gtggatgcga catcaacccg
     1021 gcggcttcta tatccgacca ggaaagcgaa cagatgttaa tcaccagtag ttcaaatggc
     1081 tgagcatcag cgggcagcga agacgggaag gtcgatttac gttttgcctc cgcgttatag
     1141 aaattattca gccaggcgtt aaggttcgcc gttgttggcg gtgcagtttg tgcgggcata
     1201 tcactcacta ccggtgcgcc acccgtcgcc gcaacggttg ccgctgcgtt accacccgtc
     1261 gttgttacag tggtcgtcgg ttgtccggct ggccacaagg agaaacttgg tcccgccagg
     1321 gtaagtacgt tcagccatag cagtatggca accacaaaaa cggtaatgcg aatccattgt
     1381 gacaggaata accaggccac taataaaaca aaaatggccc caatcatctg ccagttaata
     1441 aagcgtgtga caaggtcgat taaataatcg gtactgaacc ccgccacctg cgaaccctgg
     1501 ctcattatgc tttccgggcc aggcaaccag gtgtcatgcc agaacaaagc aaagccgatc
     1561 ggcagggcaa tccagtggcg caagcgatgc aggctgtagc gcggaatggg catcagcaga
     1621 aacgcggcaa acaccaaatt gaggagcgga tggaagttaa gatatcccgc ccacaacagg
     1681 ccgaacttaa ccagaaaata gaagttccag ccggaaaggc cgcgccagta ttgccagagg
     1741 gaagaaggca tggcggtatt ttgcgtaaat tgagtcattt tttggttgcc ctggcttttt
     1801 ccgtgcggcg taacgtcccg gccggtttaa cataacgagg tttagcaaag aacaaacgta
     1861 aggtgtcgcg aatgcgtcgc aaagagtgcc gcgccagata gcccagcggg aaaaatatca
     1921 gtgcgcaaac gacaataatt tcaatgatat cgctgatggt catcatgatg agcgctccac
     1981 agcatcatct aacagtcgca tgggttctgg tattcgtcgc cagtggcgac catcgtgctc
     2041 ggcattgatg accggtttag aactttgcgt taaaggcagc ggcatgcccc attgttctgg
     2101 ggcaagcaag cgcatctgca ctagctcggc actgatttga tcatcttcaa accagaccat
     2161 acggtttgag aaaatgtcgc cagtaggcaa tgggaaaata tgattcaacg cggtatccag
     2221 atcgttaatc cgacagaatg agagaaacag caccagccga ttaccgccaa tggtcatgat
     2281 atcgccggta cggttagggc gacacagcgt cagggcttgt tcaacgcgga tacccggtac
     2341 cggacgtagg gcaaccagaa cgcctttacc gtgggcaggt aatagagggt tattcatcat
     2401 gttgttgacg gcattacaga acacatccca cttctggaaa ccacgcagtt tgagcggctg
     2461 ggtcattgac agcaaggtag tgatatcttc cggcacatag cgactaaact tctgcccttg
     2521 cacgctttcg atcatcgtca gacaacggga gagtggcgca ttccacggaa taaccatatt
     2581 tgcaccgcag gccaataaca aacgttcatc ggtggcgcgc aggctagcgg tattttcccg
     2641 cacgaggatt ttcatcgcac taccgcgctg gcgacgcagg gtatgaatgc tgcgggccag
     2701 tggctcgatt tgcgcatttt gctgtaaaga aaagaccacc gtcgccgcct gagcggtacg
     2761 ggcttcattg aacaggactt cgttattgtt gaacagttgc cagtgttccg atagcggcgg
     2821 cgcaccttcc agtacagcaa cattactcag aatgcgtttt tcgtcgctgc gtggttggat
     2881 ctccgcctct tcgctttgaa ctaatgtcca gataccattt tgttgctgaa cgctaagctg
     2941 ctgacgggcg ctgacccctt tttcgttgca ccagaaggca atatccagca aatgttggtc
     3001 accctgaaaa cgcaaactgg caagaccaaa aagtgaacgg tattcctcaa gcaacaatga
     3061 aaattgttta tcgttattat ttccgggatt aattaccaaa agcgaacaat ggtttaacct
     3121 gctccattta ttcattttat ccaaccatga gcgaagccgc tcggcaggaa tgttttgcca
     3181 tgcattattt gcgcaaacaa gaataaaaag gtaattatgg ggatcaatag aacactgcaa
     3241 atcacgggtc aaatagtata gacctttttc atgatttagc attgaaaata attttatttt
     3301 ttccggccct tgagaatcat ctaattgaaa gattttcgcc ggatcgctgt ccatgctaat
     3361 gaccgcgacg tgtgcggttt cagcctggga tgcaattgtt tgattcgcca gactgatagc
     3421 atcttcatgg cgatcgacgt taaaccacca gacgccgcct gctggcatat gtcgcagctc
     3481 atcccataat gatgagatac cgatagagaa tacagggtcc acaatgtccc tcatgtcacc
     3541 attttgtctg attatcagtt tactagcgaa agctaagaaa taaacctacc attgaaatta
     3601 aaacttatca gatttagcat gtgaatcaat ttagaaggtc acaagacgtg tgattttagt
     3661 cttttcgttt ttaacttttt ctgttgcgta acggaaagtc aaaaagtgag caaattcccg
     3721 tctcgccgta aacaataaac cggcgaaatg ctcacggtta gaattgtctc atgaacggta
     3781 cggttatttc atagggatca agcaaaatga ataacaatga accagatact ctgcctgatc
     3841 ccgcgatagg ctatatcttc cagaatgata ttgtggcgtt aaagcaggca ttttcactgc
     3901 ctgatattga ttatgccgat atttcccaac gcgaacagtt ggccgcggca ttaaaacgct
     3961 ggccgttgct ggcagagttt gcgcaacaaa agtaggggat tggtgaatgg ccgtac
//
LOCUS       DAZKHF020000312         3992 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2201S31241
            SAMN43267800-rid25106413.denovo.314, whole genome shotgun sequence.
ACCESSION   DAZKHF020000312 DAZKHF020000000
VERSION     DAZKHF020000312.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267800
            Sequence Read Archive: SRR30307263
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3992)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3992)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 3992)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350581.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350581.2
            Genome Coverage       :: 151x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:21:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,652
            CDSs (total)                      :: 5,557
            Genes (coding)                    :: 5,320
            CDSs (with protein)               :: 5,320
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 237
            CDSs (without protein)            :: 237
            Pseudo Genes (ambiguous residues) :: 0 of 237
            Pseudo Genes (frameshifted)       :: 102 of 237
            Pseudo Genes (incomplete)         :: 130 of 237
            Pseudo Genes (internal stop)      :: 52 of 237
            Pseudo Genes (multiple problems)  :: 41 of 237
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3992
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267800-rid25106413.denovo.314"
                     /serotype="O 26:H11"
                     /isolate="2201S31241"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-01"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            complement(<1..459)
                     /locus_tag="ACHOSC_RS21985"
                     /old_locus_tag="pgaptmp_004397"
     CDS             complement(<1..459)
                     /locus_tag="ACHOSC_RS21985"
                     /old_locus_tag="pgaptmp_004397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000104949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HGU4550198.1"
                     /translation="MSTKLTGYVWDGCAASGMKLSSVAIMARLADFSNDEGVCWPSIE
                     TIARQIGAGMSTVRTAIARLEAEGWLTRKARRQGNRNASNVYQLNVAKLQAAAFSQLS
                     DSDPSKSDASKSDPSKFDASKSGKKAGFHPSESGGDPSVKSKHDPSDKKPS"
     gene            complement(449..628)
                     /locus_tag="ACHOSC_RS21990"
                     /old_locus_tag="pgaptmp_004398"
     CDS             complement(449..628)
                     /locus_tag="ACHOSC_RS21990"
                     /old_locus_tag="pgaptmp_004398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001761574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4222 domain-containing protein"
                     /protein_id="HGU4550199.1"
                     /translation="MRQVNRWFKDHYGVPVRVIRWEPETQRVIYLREGYEHECFSPLE
                     QFRRKFREIEVGHEH"
     gene            complement(804..1355)
                     /gene="ymfL"
                     /locus_tag="ACHOSC_RS21995"
                     /old_locus_tag="pgaptmp_004399"
     CDS             complement(804..1355)
                     /gene="ymfL"
                     /locus_tag="ACHOSC_RS21995"
                     /old_locus_tag="pgaptmp_004399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415665.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YmfL"
                     /protein_id="HGU4550200.1"
                     /translation="MGKHHWKVEKQPEWYVKAVRKTIAALPGGYAEAADWLDVTENAL
                     FNRLRADGDQIFPLGWAMILQRAGGTHFIADAVAQSANGVFVSLPDVEDVDNADINQR
                     LLEVIEQIGSYSKQIRSAIEDGVVEPHEKTAINDELYLSISKLQEHAALVYKIFCISE
                     SNDARECAAPGAVACRDCGETNA"
     gene            complement(1348..1608)
                     /locus_tag="ACHOSC_RS22000"
                     /old_locus_tag="pgaptmp_004400"
     CDS             complement(1348..1608)
                     /locus_tag="ACHOSC_RS22000"
                     /old_locus_tag="pgaptmp_004400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001191680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HGU4550201.1"
                     /translation="MQSPLRNVRKAHGFTLQHVAAGVQVNPATLSRIERLEQIPSIEL
                     AERLANFFKGEVSEMQILYPARFQSSQNQNGFKPQEQEVNRG"
     gene            1706..2398
                     /locus_tag="ACHOSC_RS22005"
                     /old_locus_tag="pgaptmp_004401"
     CDS             1706..2398
                     /locus_tag="ACHOSC_RS22005"
                     /old_locus_tag="pgaptmp_004401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001358750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XRE family transcriptional regulator"
                     /protein_id="HGU4550202.1"
                     /translation="MNIGNRVRQLRQAKNMKIADLAEAIGVDAANISRLETGKQKQFT
                     EQTLSNIAKSLGVDIADLFTSAHKSNTVYKNSNNEDVAQVKDVFRIEMLDISASAGNG
                     LIQGGDVIDVIHAIEYRTDNAVSMFGGRPANHIKVINVRGDSMCPTIEPGDLIFVDVS
                     INQFDGDGIYVFGFDDKIYVKRLQMIPDKLLVISDNQIYREWGITSENEHRFMVFGKV
                     LISQSQTLKRHN"
     gene            complement(2718..3233)
                     /locus_tag="ACHOSC_RS22010"
                     /old_locus_tag="pgaptmp_004402"
     CDS             complement(2718..3233)
                     /locus_tag="ACHOSC_RS22010"
                     /old_locus_tag="pgaptmp_004402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309531.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4550203.1"
                     /translation="MIAELTAAMTAIRETAQIAKLMNEAKTQAEVNAAIGELNSKLAS
                     IQRECVSLVELVGSYQEINASLKAKIAEFENFEAQTEGYILNQLESGTFVYSKEVIVN
                     GGSITMHLCPKCFGQKIVSILQPFPVSEDELFHKSRCLHCENKFLMNKNPDYVSPPSI
                     EELSRKLNGNL"
     gene            3378..3689
                     /locus_tag="ACHOSC_RS22015"
                     /old_locus_tag="pgaptmp_004403"
                     /pseudo
     CDS             3378..3689
                     /locus_tag="ACHOSC_RS22015"
                     /old_locus_tag="pgaptmp_004403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518763.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            3704..>3992
                     /gene="yfdP"
                     /locus_tag="ACHOSC_RS22020"
                     /old_locus_tag="pgaptmp_004404"
     CDS             3704..>3992
                     /gene="yfdP"
                     /locus_tag="ACHOSC_RS22020"
                     /old_locus_tag="pgaptmp_004404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416860.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YfdP"
                     /protein_id="HGU4550204.1"
                     /translation="MASERSTDVQAFIGELDGGVFETKIGAVLSEVASGVMNTKTKGK
                     VSLNLEIEPFDENRVKIKHKLSYVRPTNRGKISEEDTTETPMYVNRGGRLTI"
BASE COUNT         1066 a          974 c          898 g         1054 t
ORIGIN      
        1 agaaggtttt ttatctgacg gatcatgttt tgattttact gacggatccc cgccagattc
       61 tgacgggtga aaacccgctt ttttgccaga tttcgacgca tcaaattttg acgggtcaga
      121 ttttgatgcg tcagattttg acgggtcaga atctgacagt tgagaaaatg ccgctgcctg
      181 aagcttcgca acgttaagct gataaacatt cgacgcattg cggttaccct ggcgacgcgc
      241 cttacgcgtt aaccagcctt ctgcttccag ccgtgcgata gccgtcctga cggtactcat
      301 ccccgcgcca atctgacggg caatagtttc aattgatggc cagcacacac cttcgtcatt
      361 actgaaatca gccaggcggg ccataattgc cacgctggat aatttcatgc ctgatgcagc
      421 gcaaccatcc catacatagc cggttaattt agtgctcatg accgacctct atttccctga
      481 atttacgacg aaactgttcg agcgggctga agcactcatg ctcatagcct tcgcggaggt
      541 agataactcg ttgtgtttcc ggctcccaac gaatgactct gacgggtact ccgtagtgat
      601 ctttgaacca gcggttaact tgtcgcaaag gactgtctcc ttctgccggt taaaatcacc
      661 cacagcccac tctgcaaagc tgtgggttac aattaccctg ccacctggta cattaactgc
      721 atagcaatac tccaccttcg cttttccacc cggtacagga agcgcaatca gttgcgagcg
      781 acggtagtgt gttgttaaac tgttcatgcg ttagtttctc cacagtcacg acacgccacg
      841 gcgcccggag ctgcacactc gcgggcgtca ttactttctg aaatgcaaaa aattttgtag
      901 accagtgctg catgctcctg cagcttcgaa attgagaggt acagctcgtc gttaattgct
      961 gtcttctcat gcggttccac tacaccgtct tcaattgctg aacgaatctg ttttgaataa
     1021 ctgccgatct gttcaatgac ctccagcagg cgttggttga tatcggcgtt gtccacatcc
     1081 tcgacatcag gaagagacac aaagacgcca tttgcagact gcgccacagc gtcagcaatg
     1141 aagtgagttc caccagcacg ttgcaaaatc attgcccatc ccagcgggaa aatctgatcg
     1201 ccatcggcac gaaggcggtt aaataatgcg ttctctgtta catccagcca gtcagcagct
     1261 tcagcgtacc cccccggcaa cgctgcgata gtttttctga cagctttcac gtaccactca
     1321 ggctgttttt ctactttcca gtgatgctta cccacggttc acctcctgtt cctgtggttt
     1381 aaacccattc tggttttggc tagattgaaa acgtgccgga taaagaatct gcatttcgct
     1441 gacttcaccc ttaaaaaaat tggctaaacg ttctgcaagc tcgatagatg gaatctgctc
     1501 cagcctctca atacgactca acgtcgctgg attgacttga acacccgcag caacatgctg
     1561 caaagtgaaa ccatgcgcct tacgcacatt tcgtaatggt gattgcatac gtcctccaaa
     1621 tattgcgcgt tatgcatgtt atttcacgca attattttgc gcaagttgat ttgcttatca
     1681 cgcaataaag aaatgtaata aacgcatgaa cataggaaac cgagtcagac aacttcgcca
     1741 agcgaagaac atgaaaatcg ccgatctcgc tgaagcgata ggagtagatg cggcgaacat
     1801 ctcgcgctta gaaacgggta agcaaaaaca atttaccgaa caaacactga gtaatattgc
     1861 caagagctta ggtgttgata ttgctgatct ctttacctct gcccacaaaa gtaatactgt
     1921 atataaaaac agtaataatg aggatgttgc gcaggtgaag gatgtgttcc gtattgaaat
     1981 gctggatatc agtgccagtg cgggaaatgg ccttatccag ggcggtgatg tcattgatgt
     2041 gattcatgcc atcgaataca gaactgataa tgctgtatca atgttcggcg gacgaccagc
     2101 caatcacatc aaagttatca acgttcgtgg ggacagtatg tgtccaacca ttgagccagg
     2161 agatctcatc ttcgttgatg tcagcatcaa tcagtttgat ggtgatggta tatatgtctt
     2221 tggttttgat gacaaaatat acgttaaaag acttcaaatg attcctgaca aactgctggt
     2281 gatttctgat aaccagattt accgtgaatg gggaattact agcgaaaacg aacaccgatt
     2341 catggtcttt ggaaaggtct taatcagtca gtcgcaaacc cttaagagac ataattaacc
     2401 tcaatatccc atccatcggc caccgaaagg tggcttttta ttacctataa atttgcatat
     2461 cacgcaaata tcacttgcat atctcgcaat ttaattttat cttttgttcc agaccaacta
     2521 caggattaca acaaaatctg gttgcaacac ggtgcatgtg tcgtaagcag tcagtaaatg
     2581 tcaaaaacga acaggcagga cgcccacgaa gtagccgcct ggggcatatg aagtccagga
     2641 tgattcgtta gcaacaaaaa agcgccctac aggacgctta gctctttaac aatctggata
     2701 tccacaacag tagtaatcta cagattgccg ttaagttttc tggacaactc ctcaatggat
     2761 ggaggcgata cgtaatccgg atttttattc atcagaaact tattttcaca gtggaggcac
     2821 ctgcttttat gaaaaagctc atcttcgcta accgggaatg gttgaagtat cgatactatc
     2881 ttttgtccaa aacattttgg gcaaagatgc atggttatgc tgccaccgtt cacgattacc
     2941 tccttcgagt atacaaaagt acccgactca agttggttaa ggatatagcc ttccgtctga
     3001 gcctcaaagt tttcgaattc tgcaatttta gctttgagag aagcatttat ttcttgataa
     3061 gagcccacca gttcaacgag agacacgcat tcgcgctgaa tagacgcaag ctttgagttc
     3121 agctcaccaa tagccgcatt tacttcagct tgagtttttg cctcgttcat tagttttgca
     3181 atctgggctg tttcacgaat agccgtcatt gctgccgtta attcagcgat cacattgaat
     3241 actcttattg ttgttgggga tatccagatt aaccgaatcc ttgttgttgg ggaataacca
     3301 ggtccacctc gcctgatgtg gctaaaagca ggcacataac agctaagtat tttcaaccag
     3361 agagaatcct tagcgttgtg gtgaatgcgg ctcagcgcac gcgggttaag gttgaggctg
     3421 acagtcgacc ttctgtggat acccacccgc ctggtgtgca accttcgcca ggcaccggga
     3481 ggcacccggc accacaactt tatgctgtgt gtagtcctgg cggtaccagt ttgtaccctt
     3541 gcttccggct ggtaccgtcc ttttttgcaa aacagagaag agcatcaccg gacgacgggc
     3601 tcataaccca atccatccgg gcggctgcca ccgcaggtgt tcttctctgt tttgtggaga
     3661 aaccaaccga ccttgcaagg tcgatatgat gaggagcagc aaaatggcta gcgaacgcag
     3721 tactgatgtg caggcattta tcggggagct ggacggcggc gtatttgaaa ccaaaatcgg
     3781 cgcagttctc agtgaagtcg cttccggtgt gatgaacacg aaaaccaaag ggaaggtctc
     3841 gctcaacctg gaaatcgaac cgtttgatga gaaccgtgtg aaaatcaaac acaaactctc
     3901 atatgttcgc ccgactaacc gcgggaaaat ttccgaagaa gacaccaccg aaacgccgat
     3961 gtatgtcaat cgcggtggtc gactgactat tc
//
LOCUS       DAZKHF020000313         3985 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2201S31241
            SAMN43267800-rid25106413.denovo.315, whole genome shotgun sequence.
ACCESSION   DAZKHF020000313 DAZKHF020000000
VERSION     DAZKHF020000313.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267800
            Sequence Read Archive: SRR30307263
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3985)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3985)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 3985)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350581.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350581.2
            Genome Coverage       :: 151x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:21:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,652
            CDSs (total)                      :: 5,557
            Genes (coding)                    :: 5,320
            CDSs (with protein)               :: 5,320
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 237
            CDSs (without protein)            :: 237
            Pseudo Genes (ambiguous residues) :: 0 of 237
            Pseudo Genes (frameshifted)       :: 102 of 237
            Pseudo Genes (incomplete)         :: 130 of 237
            Pseudo Genes (internal stop)      :: 52 of 237
            Pseudo Genes (multiple problems)  :: 41 of 237
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3985
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267800-rid25106413.denovo.315"
                     /serotype="O 26:H11"
                     /isolate="2201S31241"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-01"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            52..1710
                     /gene="dpiB"
                     /locus_tag="ACHOSC_RS22025"
                     /old_locus_tag="pgaptmp_004405"
     CDS             52..1710
                     /gene="dpiB"
                     /locus_tag="ACHOSC_RS22025"
                     /old_locus_tag="pgaptmp_004405"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase DpiB"
                     /protein_id="HGU4550205.1"
                     /translation="MLQLNENKQFAFFQRLAFPLRIFLLILVFSIFVIAALAQYFTAS
                     FEDYLTLHVRDMAMNQAKIIASNDSVISAVKTRDYKRLATIANKLQRDTDFDYVVIGD
                     RHSIRLYHPNPEKIGYPMQFTKQGALEKGESYFITGKGSMGMAMRAKTPIFDDDGKVI
                     GVVSIGYLVSKIDSWRAEFLLPMAGVFVVLLGILMLLSWFLAAHIRRQMMGMEPKQIA
                     RVVRQQEALFSSVYEGLIAVDPHGYITAINRNARKMLGLSSPGRQWLGKPIAEVVRPA
                     DFFTEQIDEKRQDVVANFNGLSVIANREAIRSGDDLLGAIISFRSKDEISTLNAQLTQ
                     IKQYVESLRTLRHEHLNWMSTLNGLLQMKEYDRVLAMVQGESQAQQQLIDSLREAFAD
                     RQVAGLLFGKVQRARELGLKMIIVPGSQLSQLPPGLDSTEFAAIVGNLLDNAFEASLR
                     SDEGNKIVELFLSDEGDDVVIEVADQGCGVPESLRDKIFEQGVSTRADEPGEHGIGLY
                     LIASYVTRCGGVITLEDNDPCGTLFSIYIPKVKPNDSSINPIDR"
     gene            1679..2359
                     /gene="dpiA"
                     /locus_tag="ACHOSC_RS22030"
                     /old_locus_tag="pgaptmp_004406"
                     /gene_synonym="citB"
     CDS             1679..2359
                     /gene="dpiA"
                     /locus_tag="ACHOSC_RS22030"
                     /old_locus_tag="pgaptmp_004406"
                     /gene_synonym="citB"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component response regulator DpiA"
                     /protein_id="HGU4550206.1"
                     /translation="MTAPLTLLIVEDETPLAEMHAEYIRHIPGFSQILLAGNLAQARM
                     MIERFKPGLILLDNYLPDGRGINLLHELVQAHYPGDVVFTTAASDMETVSEAVRCGVF
                     DYLIKPIAYERLGQTLTRFRQRKHMLESIDSASQKQIDEMFNAYARGEPKDELPTGID
                     PLTLNAVRKLFKEPGVQHTAETVAQALTISRTTARRYLEYCASRHLIIAEIVHGKVGR
                     PQRIYHSG"
     gene            complement(2400..3785)
                     /gene="dcuC"
                     /locus_tag="ACHOSC_RS22035"
                     /old_locus_tag="pgaptmp_004407"
     CDS             complement(2400..3785)
                     /gene="dcuC"
                     /locus_tag="ACHOSC_RS22035"
                     /old_locus_tag="pgaptmp_004407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015556 - C4-dicarboxylate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015740 - C4-dicarboxylate transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic C4-dicarboxylate transporter DcuC"
                     /protein_id="HGU4550207.1"
                     /translation="MLTFIELLIGVVVIVGVARYIIKGYSATGVLFVGGLLLLIISAI
                     MGHKVLPSSQASTGYSATDIVEYVKILLMSRGGDLGMMIMMLCGFAAYMTHIGANDMV
                     VKLASKPLQYINSPYLLMIAAYFVACLMSLAVSSATGLGVLLMATLFPVMVNVGISRG
                     AAAAICASPAAIILAPTSGDVVLAAQASEMSLIDFAFKTTLPISIAAIIGMAIAHFFW
                     QRYLDKKEHISHEMLDVSEITTTAPAFYAILPFTPIIGVLIFDGKWGPQLHIITILVI
                     CMLIASILEFLRSFNTQKVFSGLEVAYRGMADAFANVVMLLVAAGVFAQGLSTIGFIQ
                     SLISIATSFGSASIILMLVLVILTMLAAVTTGSGNAPFYAFVEMIPKLAHSSGINPAY
                     LTIPMLQASNLGRTLSPVSGVVVAVAGMAKISPFEVVKRTSVPVLVGLVIVIVATELM
                     VPGTAAAVTGK"
BASE COUNT         1093 a          943 c         1027 g          922 t
ORIGIN      
        1 cattttaatt acatccgtca aagaggctcg ggacaacccg caaggaaaac aatgttgcag
       61 cttaacgaga ataaacagtt tgcatttttc caaagactgg catttccgct gcgtatcttt
      121 ttgctgattc tggtgttctc aatatttgtc attgcagccc tggcgcaata ttttacggcc
      181 agttttgagg actatttaac gcttcatgta cgcgacatgg caatgaatca ggcgaaaatt
      241 attgcctcca atgacagtgt catctctgcg gtgaaaacgc gtgactacaa acggctggcg
      301 accatcgcta acaaattaca aagagatacc gattttgatt atgtggtgat tggggaccgg
      361 cactcgatcc gcctttacca tcctaatccg gagaaaattg gttatcctat gcagttcacc
      421 aaacagggcg cgctggagaa aggggagagc tacttcatta ccgggaaagg gtcaatgggg
      481 atggcgatgc gcgccaaaac gccaatcttt gatgacgatg gaaaagtcat cggcgtggtg
      541 tcgattggct acctggtgag taaaatcgat agctggcggg ctgagttttt attaccgatg
      601 gcaggcgtgt ttgtcgtgct gttagggatt ctgatgttgc tgtcgtggtt cctggcagcg
      661 catatccgtc ggcaaatgat gggcatggag ccaaagcaaa tcgcacgcgt ggtccgtcag
      721 caagaggcgc tgtttagttc ggtttatgaa gggctgattg cggtggatcc gcatggttac
      781 attaccgcca tcaatcgtaa cgcaagaaag atgctggggc tgagctctcc cggacggcaa
      841 tggttgggta aacccattgc tgaagtggtc aggcccgccg atttctttac cgaacagatt
      901 gatgaaaaac gtcaggatgt ggtggctaac tttaacggtc tgagcgttat tgccaatcgg
      961 gaagctattc gttcaggtga tgatttgctg ggggccatta tcagctttcg tagtaaagac
     1021 gaaatttcca ccctcaatgc gcaactgacg caaataaaac aatacgtcga gagccttcgt
     1081 acattgcgac acgagcatct caattggatg tcgacgctca atggtctgtt gcagatgaaa
     1141 gagtatgatc gcgtgctggc gatggtgcag ggggagtctc aggcccagca acagcttatt
     1201 gacagcctgc gtgaggcgtt tgccgatcgc caggtggcgg ggctgctttt tggtaaagtg
     1261 cagcgcgccc gggaactggg gctaaaaatg atcattgtcc ccggtagcca gctttcgcaa
     1321 ctgccgccag gactggatag caccgagttt gcagccattg tgggcaattt acttgataac
     1381 gccttcgaag ccagcctgcg tagcgatgaa ggaaacaaga tcgttgaatt attcctcagc
     1441 gatgaaggcg atgatgtggt gattgaagtc gccgatcagg gctgcggcgt tccagagtct
     1501 ctacgagaca aaatatttga gcagggggtc agtacgcgtg ctgacgagcc cggtgaacat
     1561 ggcattgggt tgtacttgat tgccagctac gtaacgcgct gcggtggtgt tatcactctc
     1621 gaagataatg atccctgcgg taccttgttt tcaatctata ttccgaaagt gaaacctaat
     1681 gacagctcca ttaaccctat tgatcgttga ggacgaaacg ccgctggcag agatgcatgc
     1741 ggaatatatt cgtcacattc ccggattcag tcagatatta ctggcgggaa acctggcgca
     1801 ggcccgaatg atgatcgagc gttttaagcc ggggctaatc ttgctcgata actatcttcc
     1861 tgacggcaga gggattaatt tattgcatga actggtgcag gcgcattatc ccggcgacgt
     1921 ggtgtttacc actgcagcca gcgatatgga aacggtgtct gaagcagtac gttgtggtgt
     1981 atttgattat ctcattaagc ccattgccta tgaacggctg gggcaaacgc taacccgttt
     2041 ccgccagcgt aaacatatgc tggaaagtat tgatagcgcc agccagaagc aaattgatga
     2101 gatgtttaat gcctatgctc gcggtgaacc taaagacgaa ctgccgaccg gcattgatcc
     2161 cttgacgcta aacgcggtgc gaaaactgtt taaagagcct ggtgtgcaac atacggcaga
     2221 aacggtggcg caggcactga ccatcagtcg caccactgcc aggcgttatc ttgaatattg
     2281 cgccagccgc catctgatta ttgctgaaat tgttcacggc aaagttggca gaccgcaacg
     2341 catataccac agtgggtgac atgataaagc cggaggaaac ttgcctccgg cattactatt
     2401 tacttgcctg tgaccgctgc tgccgttcct ggcaccatca gctctgtagc aacgataaca
     2461 atcaccaaac caacaagcac cggtaccgag gtgcgtttta cgacttcaaa cggcgagatc
     2521 ttcgccatcc cggcaaccgc aacgactacg ccagaaacgg gcgaaagggt acggcccagg
     2581 tttgacgcct gcagcatcgg gatagtcaaa tacgccgggt taatgccgga agagtgcgcc
     2641 agtttcggga tcatctcaac aaacgcataa aacggcgcat tgcctgaacc ggtcgtgact
     2701 gccgccagca tggtcagaat caccaatacc agcatcagga tgatactcgc cgaaccaaac
     2761 gaggtagcga tagaaatcag actttgaata aagccgatgg tgctaagccc ctgagcgaat
     2821 accccagcgg caaccagcag catcaccacg ttagcaaacg catctgccat tccgcgataa
     2881 gccacttcca gaccagagaa aactttctgg gtattaaagc tgcggaggaa ctccagaatg
     2941 gaggcaatca gcatacaaat caccagaata gtgatgatgt gtaattgcgg accccattta
     3001 ccgtcaaaaa tcagtacacc gatgatcggc gtgaacggca aaatggcata aaacgcagga
     3061 gcagtggtgg tgatttcact gacatctaac atttcatgag agatgtgctc ttttttatcc
     3121 agataacgtt gccagaagaa gtgggcgatc gccatgccga taattgcagc aattgagata
     3181 ggcagcgtcg ttttgaaggc gaagtcaatc agcgacattt cggaagcttg cgccgccagc
     3241 accacatccc ctgaagtcgg tgcgagaata atcgccgccg gggaggcaca aatggcagca
     3301 gctgcgccac gactgatacc aacgtttacc atcaccggaa atagggttgc catcagcaaa
     3361 acacccagac cggttgcgga agagacggcc agagacatca gacaggcgac aaaataggcg
     3421 gcaatcatca gcaggtaagg ggagttaata tactgcaatg gttttgacgc cagcttgacc
     3481 accatatcat tcgcgccgat atgggtcatg taagcggcaa atccacacag catcataatc
     3541 atcatgccga ggtcgccgcc gcggctcatt agtaatattt taacgtattc aacgatatcc
     3601 gtggcgctgt agcctgttga agcctggctg gacggtaaca ctttgtgccc cataatggca
     3661 ctgataatca gcaataacag gccaccgaca aataacacac cagtggcgga atacccttta
     3721 atgatgtagc gagctacacc cacaataacc acaaccccaa taaggagttc aatgaatgtc
     3781 agcataattt ttcctgtctc caggccccaa agtaaataat aaaaaattcc taaagcttaa
     3841 ggaaaaaata tgcccaataa attggcgatg aatgctgatt aaaatcaaga aaaactgcca
     3901 ttaagacatt gaagttgctg tttttataca tagataacaa aacgcatatt tagcggttgg
     3961 caattatcac ataataatgt gtgta
//
LOCUS       DAZKHF020000314         3934 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2201S31241
            SAMN43267800-rid25106413.denovo.316, whole genome shotgun sequence.
ACCESSION   DAZKHF020000314 DAZKHF020000000
VERSION     DAZKHF020000314.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267800
            Sequence Read Archive: SRR30307263
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3934)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3934)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 3934)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350581.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350581.2
            Genome Coverage       :: 151x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:21:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,652
            CDSs (total)                      :: 5,557
            Genes (coding)                    :: 5,320
            CDSs (with protein)               :: 5,320
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 237
            CDSs (without protein)            :: 237
            Pseudo Genes (ambiguous residues) :: 0 of 237
            Pseudo Genes (frameshifted)       :: 102 of 237
            Pseudo Genes (incomplete)         :: 130 of 237
            Pseudo Genes (internal stop)      :: 52 of 237
            Pseudo Genes (multiple problems)  :: 41 of 237
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3934
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267800-rid25106413.denovo.316"
                     /serotype="O 26:H11"
                     /isolate="2201S31241"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-01"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            146..658
                     /gene="secM"
                     /locus_tag="ACHOSC_RS22040"
                     /old_locus_tag="pgaptmp_004408"
     CDS             146..658
                     /gene="secM"
                     /locus_tag="ACHOSC_RS22040"
                     /old_locus_tag="pgaptmp_004408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414639.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0045182 - translation regulator activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="secA translation cis-regulator SecM"
                     /protein_id="HGU4550208.1"
                     /translation="MSGILTRWRQFGKRYFWPHLLLGMVAASLGLPALSNAAEPNAPA
                     KATTRNHEPSAKVNFGQLALLEANTRRPNSNYSVDYWHQHAIRTVIRHLSFAMAPQTL
                     PVAEESLPLQAQHLALLDTLSALLTQEGTPSEKGYRIDYAHFTPQAKFSTPVWISQAQ
                     GIRAGPQRLT"
     gene            720..3425
                     /gene="secA"
                     /locus_tag="ACHOSC_RS22045"
                     /old_locus_tag="pgaptmp_004409"
     CDS             720..3425
                     /gene="secA"
                     /locus_tag="ACHOSC_RS22045"
                     /old_locus_tag="pgaptmp_004409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006605 - protein targeting [Evidence IEA];
                     GO_process: GO:0006886 - intracellular protein transport
                     [Evidence IEA];
                     GO_process: GO:0017038 - protein import [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecA"
                     /protein_id="HGU4550209.1"
                     /translation="MLIKLLTKVFGSRNDRTLRRMRKVVNIINAMEPEMEKLSDEELK
                     GKTAEFRARLEKGEVLENLIPEAFAVVREASKRVFGMRHFDVQLLGGMVLNERCIAEM
                     RTGEGKTLTATLPAYLNALTGKGVHVVTVNDYLAQRDAENNRPLFEFLGLTVGINLPG
                     MPAPAKREAYAADITYGTNNEYGFDYLRDNMAFSPEERVQRKLHYALVDEVDSILIDE
                     ARTPLIISGPAEDSSEMYKRVNKIIPHLIRQEKEDSETFQGEGHFSVDEKSRQVNLTE
                     RGLVLIEELLVKEGIMDEGESLYSPANIMLMHHVTAALRAHALFTRDVDYIVKDGEVI
                     IVDEHTGRTMQGRRWSDGLHQAVEAKEGVQIQNENQTLASITFQNYFRLYEKLAGMTG
                     TADTEAFEFSSIYKLDTVVVPTNRPMIRKDLPDLVYMTEAEKIQAIIEDIKERTAKGQ
                     PVLVGTISIEKSELVSNELTKAGIKHNVLNAKFHANEAAIVAQAGYPAAVTIATNMAG
                     RGTDIVLGGSWQAEVAALENPTVEQIEKIKADWQVRHDAVLEAGGLHIIGTERHESRR
                     IDNQLRGRSGRQGDAGSSRFYLSMEDALMRIFASDRVSGMMRKLGMKPGEAIEHPWVT
                     KAIANAQRKVESRNFDIRKQLLEYDDVANDQRRAIYSQRNELLDVSDVSETINSIRED
                     VFKATIDAYIPPQSLEEMWDIPGLQERLKNDFDLDLPIAEWLDKEPELHEETLRERIL
                     AQSIEVYQRKEEVVGAEMMRHFEKGVMLQTLDSLWKEHLAAMDYLRQGIHLRGYAQKD
                     PKQEYKRESFSMFAAMLESLKYEVISTLSKVQVRMPEEVEELEQQRRMEAERLAQMQQ
                     LSHQDDDSAAAAALAAQTGERKVGRNDPCPCGSGKKYKQCHGRLQ"
     gene            3485..3874
                     /gene="mutT"
                     /locus_tag="ACHOSC_RS22050"
                     /old_locus_tag="pgaptmp_004410"
     CDS             3485..3874
                     /gene="mutT"
                     /locus_tag="ACHOSC_RS22050"
                     /old_locus_tag="pgaptmp_004410"
                     /EC_number="3.6.1.55"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008413 - 8-oxo-7,8-dihydroguanosine
                     triphosphate pyrophosphatase activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="8-oxo-dGTP diphosphatase MutT"
                     /protein_id="HGU4550210.1"
                     /translation="MKKLQIAVGIIRNENNEIFITRRAADAHMANKLEFPGGKIEMGE
                     TPEQAVVRELQEEVGITPQHFSLFEKLEYEFPDRHITLWFWLVESWEGEPWGKEGQPG
                     EWMSLVGLNADDFPPANEPVIAKLKRL"
BASE COUNT         1042 a          969 c         1055 g          868 t
ORIGIN      
        1 tataagagct ataaaaaacg gtgcgaacgc tgttttctta agcacttttc cgcacaactt
       61 atcttcattc gtgctgtgga ctgcaggctt taatgataag atttgtgcgc taaatacgtt
      121 tgaatacgat cgggatggca ataacgtgag tggaatactg acgcgctggc gacagtttgg
      181 taaacgctac ttctggccgc atctcttatt agggatggtt gcggcgagtt taggtttgcc
      241 tgcgctcagc aacgccgccg aaccaaacgc gcccgcaaaa gcgacaaccc gcaaccacga
      301 gccttcagcc aaagttaact ttggtcaatt ggccttgctg gaagcgaaca cacgccgccc
      361 gaattcgaac tattccgttg attactggca tcaacatgcc attcgcacgg taatccgtca
      421 tctttctttc gcaatggcac cgcaaacact gcccgttgct gaagaatctt tgcctcttca
      481 ggcgcaacat cttgcattac tggatacgct cagcgcgctg ctgacccagg aaggcacgcc
      541 gtctgaaaag ggttatcgca ttgattatgc gcattttacc ccacaagcaa aattcagcac
      601 gcccgtctgg ataagccagg cgcaaggcat ccgtgctggc cctcaacgcc tcacctaaca
      661 acaataaacc tttacttcat tttattaact ccgcaacgcg gggcgtttga gattttatta
      721 tgctaatcaa attgttaact aaagttttcg gtagtcgtaa cgatcgcacc ctgcgccgga
      781 tgcgcaaagt ggtcaacatc atcaatgcca tggaaccgga gatggaaaaa ctctccgacg
      841 aagaactgaa agggaaaacc gcagagtttc gtgcacgtct ggaaaaaggc gaagtgctgg
      901 aaaatctgat cccggaagct ttcgccgtgg tacgtgaggc aagtaagcgc gtctttggta
      961 tgcgtcactt cgacgttcag ttactcggcg gtatggttct taacgaacgc tgcatcgccg
     1021 aaatgcgtac cggtgaaggt aaaaccctga ccgcaacgct gcctgcttac ctgaacgcac
     1081 taaccggtaa aggcgtgcac gtagttaccg tcaacgacta cctggcgcaa cgtgacgccg
     1141 aaaacaaccg tccgctgttt gaattccttg gcctgactgt cggtatcaac ctgccgggca
     1201 tgccagcacc ggcaaagcgc gaagcttacg cagctgacat cacttacggt acgaacaacg
     1261 aatacggctt tgactacctg cgcgacaata tggcgttcag ccctgaagaa cgtgtacagc
     1321 gtaaactgca ctatgcgctg gtggacgaag tggactccat cctgatcgat gaagcgcgta
     1381 caccgctgat catttccggc ccggcagaag acagctcgga aatgtataaa cgcgtgaata
     1441 aaattattcc gcacctgatc cgtcaggaaa aagaagactc cgaaaccttc cagggcgaag
     1501 gccacttctc ggtggacgaa aaatctcgcc aggtgaacct gaccgaacgt ggtctggtgc
     1561 tgattgaaga actgctggtg aaagagggca tcatggatga aggggagtct ctgtactctc
     1621 cggccaacat catgctgatg caccacgtaa cggcggcgct gcgcgctcat gcgctgttta
     1681 cccgcgacgt cgactacatc gttaaagatg gtgaagttat catcgttgac gaacacaccg
     1741 gtcgtaccat gcagggccgt cgctggtccg atggtctgca ccaggctgtg gaagcgaaag
     1801 aaggtgtgca gatccagaac gaaaaccaaa cgctggcttc gatcaccttc cagaactact
     1861 tccgtctgta tgaaaaactg gcggggatga ccggtactgc tgataccgaa gctttcgaat
     1921 ttagctccat ctacaagctg gataccgtcg ttgttccgac caaccgtcca atgattcgta
     1981 aagatctgcc ggacctggtc tacatgactg aagcggaaaa aattcaggcg atcattgaag
     2041 atatcaaaga acgtactgcg aaaggccagc cggtgctggt gggtactatc tccatcgaaa
     2101 aatcggagct ggtgtcaaac gaactgacca aagccggtat taagcacaac gtcctgaacg
     2161 ccaaattcca cgccaacgaa gcggcgattg ttgctcaggc aggttatccg gctgcggtga
     2221 ctatcgcgac caatatggcg ggtcgtggta cagatattgt gctcggtggt agctggcagg
     2281 cagaagttgc cgcgctggaa aatccgaccg tagagcaaat tgaaaaaatt aaagccgact
     2341 ggcaggtacg tcacgatgcg gtactggaag caggtggcct gcatatcatc ggtaccgagc
     2401 gtcacgaatc ccgtcgtatc gataaccagt tgcgcggtcg ttctggtcgt cagggggatg
     2461 ctggttcttc ccgtttctac ctgtcgatgg aagatgcgct gatgcgtatt tttgcttccg
     2521 accgagtatc cggcatgatg cgtaaactgg gtatgaagcc aggcgaagcc attgaacacc
     2581 cgtgggtgac taaagcgatt gccaacgccc agcgtaaagt tgaaagtcgt aacttcgaca
     2641 ttcgtaagca actgctggaa tatgatgacg tggctaacga tcagcgtcgc gccatttact
     2701 cccagcgtaa cgaactgctg gatgtcagcg atgtgagcga aaccatcaac agcattcgtg
     2761 aagatgtgtt caaagccacc attgatgcct acattccgcc acagtcgctg gaagaaatgt
     2821 gggatattcc ggggctgcag gaacgtctga agaacgattt cgacctcgat ttaccaattg
     2881 ccgagtggct ggataaagaa ccagaactgc atgaagagac gctgcgtgag cgcattctgg
     2941 cgcagtccat cgaagtgtat cagcgtaaag aagaagtggt tggtgctgag atgatgcgtc
     3001 acttcgagaa aggcgtcatg ctgcaaacgc ttgactccct gtggaaagag cacctggcag
     3061 cgatggacta tctgcgtcag ggtatccacc tgcgtggcta tgcacagaaa gatccgaagc
     3121 aggaatacaa acgtgaatcg ttctccatgt ttgcagcgat gctggagtcg ttaaaatatg
     3181 aagttatcag cacgctgagc aaagtccagg tacgtatgcc tgaagaggtt gaggagctgg
     3241 aacaacagcg tcgtatggaa gccgagcgtt tagcgcaaat gcagcagctt agccatcagg
     3301 atgacgactc tgcagccgca gctgcactgg cggcgcaaac cggtgagcgc aaagtaggac
     3361 gtaacgatcc ttgcccgtgc ggttctggta aaaaatacaa gcagtgccat ggtcgcctgc
     3421 aataaaagct aacagttgaa gtaaaaggcg caggattctg cgcctttttt ataggtttaa
     3481 gacaatgaaa aagctgcaaa ttgcggtagg tattattcgc aacgagaaca atgaaatctt
     3541 tataacgcgt cgcgcagcag atgcgcacat ggcgaataaa ctggagtttc ccggcggtaa
     3601 aattgaaatg ggcgaaacgc cggaacaggc ggtggtgcgt gaacttcagg aagaagtcgg
     3661 gattacccca caacattttt cgctatttga aaaactggaa tatgaattcc cggacaggca
     3721 tataactctg tggttctggc tggtcgaaag ctgggaaggg gagccgtggg gtaaagaagg
     3781 gcaacccggt gagtggatgt cgctagtcgg tcttaatgcc gatgattttc cgccagccaa
     3841 tgaaccggta attgcgaagc ttaagcgtct gtaggtcgga taaggcgttc acgccgcatc
     3901 cgacatttgc acaagatgcc tgatgcgacg ctgt
//
LOCUS       DAZKHF020000315         3929 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2201S31241
            SAMN43267800-rid25106413.denovo.317, whole genome shotgun sequence.
ACCESSION   DAZKHF020000315 DAZKHF020000000
VERSION     DAZKHF020000315.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267800
            Sequence Read Archive: SRR30307263
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3929)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3929)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 3929)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350581.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350581.2
            Genome Coverage       :: 151x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:21:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,652
            CDSs (total)                      :: 5,557
            Genes (coding)                    :: 5,320
            CDSs (with protein)               :: 5,320
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 237
            CDSs (without protein)            :: 237
            Pseudo Genes (ambiguous residues) :: 0 of 237
            Pseudo Genes (frameshifted)       :: 102 of 237
            Pseudo Genes (incomplete)         :: 130 of 237
            Pseudo Genes (internal stop)      :: 52 of 237
            Pseudo Genes (multiple problems)  :: 41 of 237
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3929
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267800-rid25106413.denovo.317"
                     /serotype="O 26:H11"
                     /isolate="2201S31241"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-01"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            55..2316
                     /locus_tag="ACHOSC_RS22055"
                     /old_locus_tag="pgaptmp_004411"
     CDS             55..2316
                     /locus_tag="ACHOSC_RS22055"
                     /old_locus_tag="pgaptmp_004411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415292.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydratase"
                     /protein_id="HGU4550211.1"
                     /translation="MIKLSEKGVFLASNNEIIAEEHFTGEIKKEEAKKGTIAWSILSS
                     HNTSGNMDKLKIKFDSLASHDITFVGIVQTAKASGMERFPLPYVLTNCHNSLCAVGGT
                     INGDDHVFGLSAAQRYGGIFVPPHIAVIHQYMREMMAGGGKMILGSDSHTRYGALGTM
                     AVGEGGGELVKQLLNDTWDIDYPGVVAVHLTGKPAPYVGPQDVALAIIGAVFKNGYVK
                     NKVMEFVGPGVSALSTDFRNSVDVMTTETTCLSSVWQTDEEVHNWLALHGRGQDYCQL
                     NPQPMAYYDGCISVDLSAIKPMIALPFHPSNVYEIDTLNQNLTDILREIEIESERVAH
                     GKAKLSLLDKVENGRLKVQQGIIAGCSGGNYENVIAAANALRGQSCGNDTFSLAVYPS
                     SQPVFMDLAKKGVVADLIGAGAIIRTAFCGPCFGAGDTPINNGLSIRHTTRNFPNREG
                     SKPANGQMSAVALMDARSIAATAANGGYLTSASELDCWDNVPEYAFDVTPYKNRVYQG
                     FVKGATQQPLIYGPNIKDWPELGALTDNIVLKVCSKILDEVTTTDELIPSGETSSYRS
                     NPIGLAEFTLSRRDPGYVGRSKATAELENQRLAGNVSELTEVFARIKQIAGQEHIDPL
                     QTEIGSMVYAVKPGDGSAREQAASCQRVIGGLANIAEEYATKRYRSNVINWGMLPLQM
                     AEVPTFEVGDYIYIPGIKAALDNPGTTFKGYVIHEDAPVTEITLYMESLTAEEREIIK
                     AGSLINFNKNRQM"
     gene            complement(2550..3833)
                     /locus_tag="ACHOSC_RS22060"
                     /old_locus_tag="pgaptmp_004412"
     CDS             complement(2550..3833)
                     /locus_tag="ACHOSC_RS22060"
                     /old_locus_tag="pgaptmp_004412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030599 - pectinesterase activity
                     [Evidence IEA];
                     GO_process: GO:0042545 - cell wall modification [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative acyl-CoA thioester hydrolase"
                     /protein_id="HGU4550212.1"
                     /translation="MNTFSVSRLALALAFGVTLTACSSTPPDQRPSDQTAPGTSSRPI
                     LSAKEAQNFDAQHYFASLTPGAAAWNPSPITLPAQPDFVVGPAGTQGVTHTTIQAAVD
                     AAIIKRTNKRQYIAVMPGEYQGTVYVPAAPGGITLYGTGEKPIDVKIGLSLDGGMSPA
                     DWRHDVNPRGKYMPGKPAWYMYDSCQSKRSDSIGVLCSAVFWSQNNGLQLQNLTIENT
                     LGDSVDAGNHPAVALRTDGDQVQINNVNILGRQNTFFVTNSGVQNRLETNRQPRTLVT
                     NSYIEGDVDIVSGRGAVVFDNTEFRVVNSRTQQEAYVFAPATLSNIYYGFLAVNSRFN
                     AFGDGVAQLGRSLDVDANTNGQVVIRDSAINEGFNTAKPWADAVISNRPFAGNTGSVD
                     DNDEIQRNLNDTNYNRMWEYNNRGVGSKVVAEAKK"
BASE COUNT          955 a          977 c         1050 g          947 t
ORIGIN      
        1 tccatttatt atctttctgc gcacttcacg gtgcgcagat atctggagca tttgatgatc
       61 aagttatctg aaaaaggcgt gtttctcgcc agtaataacg aaataattgc cgaagaacat
      121 ttcaccggcg aaattaaaaa agaagaagcc aaaaaaggca ctattgcctg gtctattctc
      181 tcttcgcata atacgtccgg aaatatggat aaacttaaaa ttaagtttga ttcattagcc
      241 tctcacgata ttacctttgt tggtattgta cagaccgcta aagcgtccgg tatggaacgt
      301 ttcccgctgc cgtatgtgct gaccaactgc cataactcac tctgcgccgt cggcggcact
      361 attaacggtg atgaccatgt ttttggttta tcggcggccc agcgttatgg cggtattttt
      421 gtgcctccgc atattgcggt catccatcaa tatatgcgtg agatgatggc aggcggcggc
      481 aaaatgatcc tcgggtcaga cagccacacc cgttacggtg cattagggac aatggcagtc
      541 ggtgagggcg gcggtgagtt ggtaaaacag ctgcttaatg acacctggga tatcgactat
      601 ccgggcgtgg ttgcggtgca tctgaccgga aaacctgcgc cgtatgtggg gccacaggat
      661 gtggcgctgg ctatcattgg cgcggtgttc aaaaacggtt acgtcaaaaa caaagtcatg
      721 gagttcgttg gaccgggcgt tagcgcgctc tctaccgatt tccgtaacag cgttgacgtg
      781 atgaccacgg aaaccacctg tttaagctcc gtctggcaaa ctgatgaaga agtgcataac
      841 tggctggcgc tgcacggtcg cggccaggat tactgccagc ttaaccctca accgatggcg
      901 tactacgatg gctgcatcag cgttgattta agcgccatca aaccaatgat tgcgctgccg
      961 ttccacccga gcaacgtgta tgaaatcgac acactgaacc agaacctgac cgacattctg
     1021 cgtgagattg aaattgagtc cgaacgcgtg gcgcacggta aagccaaact ctcgctgctg
     1081 gataaagtgg aaaatggtcg cctgaaagtg cagcagggga ttatcgcggg ctgttctggc
     1141 ggtaactacg aaaacgtcat cgcggcggcg aatgcactgc gcggtcaatc ctgtggcaat
     1201 gacaccttct cgctggcagt ttacccgtca tcacagccgg tgtttatgga tctcgccaaa
     1261 aaaggtgtgg tagcagattt gattggcgca ggcgcaatca tcagaaccgc gttctgcggc
     1321 ccatgctttg gcgcgggcga tacgccaatc aacaacggtt tgagtattcg ccacaccacg
     1381 cgtaacttcc cgaaccgcga aggctctaag ccagctaatg ggcagatgtc agcggtggcg
     1441 ttgatggacg ctcgttctat cgctgcgact gcggcaaacg gtggctattt aacctctgcc
     1501 agcgaacttg attgctggga caacgtgccg gagtacgcct tcgatgtaac gccgtataaa
     1561 aaccgtgttt atcagggctt tgtgaaaggg gcaactcagc aaccgctgat ttacggaccg
     1621 aacattaaag actggccgga attgggtgcg ctgactgaca atatcgtcct gaaagtgtgc
     1681 tcgaagatcc tcgacgaagt gaccaccacc gacgaactga ttccttccgg tgaaacctct
     1741 tcttatcgtt caaatccgat tggtctggcg gagtttaccc tgtcacgccg cgatcccggt
     1801 tatgttggca gaagtaaagc gactgctgag ctggaaaatc agcgtctggc ggggaatgtc
     1861 agcgagctga cagaggtgtt tgcgcgcatt aagcagattg ctggtcagga gcatattgat
     1921 ccgctgcaaa ctgaaattgg cagcatggta tatgcggtga aaccaggcga tggttctgcg
     1981 cgtgaacagg cggcgagctg ccagcgtgtg attggcggtc tggcgaatat tgccgaagag
     2041 tacgcgacta aacgctaccg ttctaacgtc atcaactggg ggatgttacc gctgcagatg
     2101 gcggaagtgc caacctttga agtgggggat tacatttaca tccctggcat taaagcggcg
     2161 ctggataatc cgggtacgac gtttaaaggt tatgtgatcc atgaagatgc gccggtaacg
     2221 gaaattacgc tctatatgga aagtctgact gctgaagagc gcgagattat caaggcgggt
     2281 agtttgatta acttcaataa aaaccgtcag atgtaaaaag cgccatgtga atgtaggtcg
     2341 cattcggcac ttattgtcgg atgcgatgct tgcgcatctt atccgaccta cgaatcgcat
     2401 cgaatctgta ggccagataa ggcattttcg cagcatccgg cacttattgt cggatgcgat
     2461 gcttgcgcat cttatccgac ctacaaatcg catcgaaccg taggccggat aaggcgttta
     2521 cgccgcatcc ggcaaatagt taattgctct tacttcttcg cctctgcaac cactttacta
     2581 cccacgccgc ggttattgta ttcccacatg cggttgtagt tagtgtcatt cagattgcgc
     2641 tgtatttcgt cgttatcatc tacgctgccg gtattacccg caaacggacg attagagatc
     2701 accgcatcgg cccacggttt agccgtgtta aaaccttcgt tgatggcgct atcacggatc
     2761 accacctgac cgttggtatt ggcatcaaca tccagcgagc ggcccagttg cgccacacca
     2821 tcaccgaaag cattgaaacg gctgtttacg gcgaggaaac cgtagtaaat gttggacagc
     2881 gtagccggtg caaacacata cgcttcttgc tgagtacgtg agttcaccac gcggaattcg
     2941 gtgttatcga acaccactgc gccgcgacca gaaacgatat ccacatcccc ttcaatgtag
     3001 ctgttggtca ccagcgtacg cggctgacga ttcgtttcca gacggttctg cacaccgctg
     3061 ttggtgacaa agaaggtgtt ctgacgaccg agaatgttaa cgttgttaat ctgtacctgg
     3121 tcaccatcag tacgcagtgc caccgccgga tggttacctg catctacgct atcgcccagc
     3181 gtgttttcga tggtcagatt ttgcagttgc aggccattgt tttgtgacca gaagaccgca
     3241 gagcagagaa caccgatact gtcgctgcgt ttgctctggc agctatcgta catataccac
     3301 gctggtttac ctggcatata tttgccgcgc gggttgacgt cgtgacgcca gtcggcaggg
     3361 ctcatgccac catcaaggga aagcccaatc ttcacatcaa tcggtttttc acctgtaccg
     3421 tacagagtaa ttccacccgg agcggcaggg acatataccg ttccctgata ctcaccaggc
     3481 atcacggcaa tatactggcg cttgttggta cgcttgataa ttgccgcatc taccgccgcc
     3541 tgaatcgtgg tatgcgttac accttgagtg cccgccgggc cgacaacaaa gtcaggttgc
     3601 gcaggcaggg taatcgggga aggattccac gctgctgcac ctggtgtcag ggatgcaaaa
     3661 tagtgttgag catcgaaatt ctgcgcttct tttgccgaca gaatcgggcg agaagaggta
     3721 ccaggcgcgg tttgatcaga aggacgttga tcgggcgggg ttgagctaca ggcggtcagc
     3781 gtcacgccaa aagccaatgc cagcgccaga cgggaaactg aaaatgtgtt cacaggttgc
     3841 tccgggctat gaaatagaaa aatgaatccg ttgaagcctg cttttttata ctaagttggc
     3901 attataaaaa agcattgctt atcaatttg
//
LOCUS       DAZKHF020000316         3924 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2201S31241
            SAMN43267800-rid25106413.denovo.318, whole genome shotgun sequence.
ACCESSION   DAZKHF020000316 DAZKHF020000000
VERSION     DAZKHF020000316.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267800
            Sequence Read Archive: SRR30307263
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3924)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3924)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 3924)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350581.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350581.2
            Genome Coverage       :: 151x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:21:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,652
            CDSs (total)                      :: 5,557
            Genes (coding)                    :: 5,320
            CDSs (with protein)               :: 5,320
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 237
            CDSs (without protein)            :: 237
            Pseudo Genes (ambiguous residues) :: 0 of 237
            Pseudo Genes (frameshifted)       :: 102 of 237
            Pseudo Genes (incomplete)         :: 130 of 237
            Pseudo Genes (internal stop)      :: 52 of 237
            Pseudo Genes (multiple problems)  :: 41 of 237
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3924
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267800-rid25106413.denovo.318"
                     /serotype="O 26:H11"
                     /isolate="2201S31241"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-01"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            <1..551
                     /gene="ykgH"
                     /locus_tag="ACHOSC_RS22065"
                     /old_locus_tag="pgaptmp_004413"
     CDS             <1..551
                     /gene="ykgH"
                     /locus_tag="ACHOSC_RS22065"
                     /old_locus_tag="pgaptmp_004413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="protein YkgH"
                     /protein_id="HGU4550213.1"
                     /translation="LYINKDNIKVSYSLKINQTTPGILVNCDSNNNFACQTTMTEDVI
                     QRITTFFHTSPDVKNREIKLEWSGDKRDLPTAEAEISRVQASIIKWYASEYHNGRQVL
                     DEIQTPSAINSELYTKMIYLTRNWSLYPNGDGCVTISSPEIKNKYPAAICLALGFFLS
                     IVISVMFCLVKKMVDEYQQNSG"
     gene            complement(797..1492)
                     /locus_tag="ACHOSC_RS22070"
                     /old_locus_tag="pgaptmp_004414"
     CDS             complement(797..1492)
                     /locus_tag="ACHOSC_RS22070"
                     /old_locus_tag="pgaptmp_004414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414842.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactate utilization protein C"
                     /protein_id="HGU4550214.1"
                     /translation="MDNRGEFLNNVAQALGRPLRLEPQAEDAPLNNYANERLTQLNQQ
                     QRCDAFIQFASDVMLTRCELTSEAKAAEAAIRLCKELGDQSVVISGDTRLEELGISER
                     LQQECNAVVWDPAKGAENISQAEQAKVGVVYAEYGLTESGGVVLFSAAGRGRSLSLLP
                     ESSLFILRKSTILPRVAQLAEKLHQKAQAGERMPSCINIISGPSSTADIELIKVVGVH
                     GPVKAVYLIIEDC"
     gene            complement(1485..2912)
                     /locus_tag="ACHOSC_RS22075"
                     /old_locus_tag="pgaptmp_004415"
     CDS             complement(1485..2912)
                     /locus_tag="ACHOSC_RS22075"
                     /old_locus_tag="pgaptmp_004415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0019516 - lactate oxidation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LutB/LldF family L-lactate oxidation iron-sulfur
                     protein"
                     /protein_id="HGU4550215.1"
                     /translation="MSIKTSNTDFKTRIRQQIEDPIMRKAVANAQQRIGANRQKMVDE
                     LGHWEEWRDRAAQIRDHVLSNLDAYLYQLSEKVTQNGGHVYFAKTKEDATRYILQVAQ
                     RKNARKVVKSKSMVTEEIGVNHVLQDAGIQVIETDLGEYILQLDQDPPSHVVVPAIHK
                     DRHQIRRVLHERLGYEGPETPEAMTLFIRQKIREDFLSAEIGITGCNFAVAETGSVCL
                     VTNEGNARMCTTLPKTHIAVMGMERIAPTFAEVDVLITMLARSAVGARLTGYNTWLTG
                     PREAGHVDGPEEFHLVIVDNGRSEVLASEFRDVLRCIRCGACMNTCPAYRHIGGHGYG
                     SIYPGPIGAVISPLLGGYKDFKDLPYACSLCTACDSVCPVRIPLSKLILRHRRVMAEK
                     GITAKAEQRAIKMFAYANSHPGLWKVGMMAGAHAASWFINGGKTPLKFGAISDWMEAR
                     DLPEADGESFRSWFKKHQAQEKKNG"
     gene            complement(2923..3642)
                     /locus_tag="ACHOSC_RS22080"
                     /old_locus_tag="pgaptmp_004416"
     CDS             complement(2923..3642)
                     /locus_tag="ACHOSC_RS22080"
                     /old_locus_tag="pgaptmp_004416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(Fe-S)-binding protein"
                     /protein_id="HGU4550216.1"
                     /translation="MNVNFFVTCIGDALKSRMARDSVLLLEKLGCRVNFPEKQGCCGQ
                     PAINSGYIKEAIPGMKNLIAALEDNDDPIISPAGSCTYAVKSYPTYLADEPEWASRAE
                     KVAARMQDLTSFIVNKLGVVDVGASLQGRAVYHPSCSLARKLGVKDEPLTLLKNVRGL
                     ELLTFAEQDTCCGFGGTFSVKMAEISGEMVKEKVVHLMEVRPEYLIGADVSCLLNISG
                     RLQREGQKVKVMHIAEVLMSR"
BASE COUNT         1035 a         1043 c          817 g         1029 t
ORIGIN      
        1 ttctgtatat caataaagat aatataaaag tgagttacag cctaaaaata aaccagacaa
       61 caccaggcat acttgttaac tgtgatagca ataataactt tgcctgtcag accacaatga
      121 ctgaagatgt tatacagcga attactacat tttttcacac cagcccagat gtcaagaaca
      181 gagaaataaa gctggaatgg tcaggagata agagagattt accaactgct gaagcggaaa
      241 tatctcgcgt gcaggcgtct attatcaaat ggtatgcgtc agaataccat aatggcaggc
      301 aagttctcga tgagatacaa acaccttcag caattaacag tgagctttat acaaaaatga
      361 tatacctgac caggaactgg tcattgtatc cgaacggtga tggctgtgta actataagct
      421 caccagaaat aaaaaataaa taccctgctg ccatttgcct ggctctggga ttttttctaa
      481 gcattgtaat ttctgtaatg ttttgccttg tcaaaaaaat ggtagatgaa taccaacaaa
      541 actctgggta gtaataaaca gcattctgat agagagtgcc tgtataaagc tgaagtgaca
      601 atgaccggga agggaacccg ttttcaggaa tggatgcctg tctttttcat tgtaaatatc
      661 ctggcattac taatgccagg atatttacag aagagcagca taaccatgac ggaacggggt
      721 taagtcaggt tgtccttaac gctattttca acctcgtatg ccttcttcag gtttatgttc
      781 agatctcata tctctctcag caatcctcaa taatcagata caccgctttc accggaccat
      841 gaaccccgac gactttgata agctcaatat ccgccgttga actagggccg ctaatgatgt
      901 taatgcagga cggcattcgt tcaccggcct gcgctttctg atgcaatttt tctgcgagtt
      961 gcgctacacg cggcaagata gtgcttttac gcaggataaa aagagaagat tccgggagca
     1021 ggctcaatga acgcccgcgc ccggcggcgg aaaaaagaac cacgcctccc gattcggtta
     1081 aaccatattc agcatacacg acacccactt tagcctgctc ggcctgcgag atattctcgg
     1141 cacctttcgc cggatcccaa acaacggcat tgcattcctg ctgcaaacgt tcactaatcc
     1201 ccaattcctc cagcctcgtg tcaccgctaa tcacgaccga ctgatctccc agctctttac
     1261 acagacgtat tgcagcttct gccgccttcg cctcgctggt cagctcacag cgcgtcaaca
     1321 taacatcgct ggcaaactga ataaacgcgt cacagcgctg ctgttggtta agttgggtaa
     1381 gccgctcgtt agcatagttg ttaagcggcg catcttctgc ttgcggttca agtcgcagcg
     1441 ggcgacccag tgcctgagca acgttattca aaaattcgcc tcgattatcc attctttttc
     1501 tcctgcgcct gatgtttctt aaaccaacta cggaaactct ctccgtcagc ttcaggaaga
     1561 tcgcgtgctt ccatccagtc gctaatcgcg ccaaatttga gtggtgtttt gccgccattg
     1621 ataaaccagc ttgccgcatg agcgccggcc atcatcccga ctttccacaa tcctggatga
     1681 ctattggcat aagcgaacat ttttatcgcc cgttgctctg cttttgcggt gatacctttt
     1741 tcagccatca cccgacgatg acgcaaaatc agttttgaca gcggaatacg caccggacac
     1801 acgctgtcac aagcagtgca taaagagcag gcgtagggta aatctttaaa atctttatag
     1861 ccgccaagta gcggagaaat caccgcacca attggccctg gataaataga gccatatcca
     1921 tgaccgccaa tatggcgata tgccggacaa gtattcatac aagccccgca gcgaatacag
     1981 cgcagcacat cccgaaattc agaggccagc acctcagaac gcccgttatc gacaataacc
     2041 agatgaaact cttcaggacc atcaacgtgc cccgcttcgc gcggtcctgt cagccaggtg
     2101 ttgtatcccg tcaaacgtgc accaacggca ctgcgcgcca gcatggtgat caatacatct
     2161 acctcggcaa acgtgggggc aatacgctcc attcccatca ctgcaatatg cgttttaggc
     2221 agcgtggtac acattcgcgc attaccttca ttggtcacca ggcataccga acctgtctct
     2281 gccaccgcga aattacagcc ggtaatacct atttcagcac tgaggaaatc ttcgcggatt
     2341 ttttgccgga tgaataaggt catcgcttca ggcgtttccg gcccctcata gcccagacgt
     2401 tcgtgtagca ctcgacggat ctgatggcga tctttatgaa ttgccgggac cacaacatga
     2461 gatggcggat cttgatccag ctggagaatg tattcaccca gatcggtttc aatcacctga
     2521 atgccagcat cctgcaacac atgattgaca ccaatctctt cggtcaccat cgatttagat
     2581 ttcaccacct tccgggcatt tttgcgttgg gcaacctgta aaatgtagcg agtggcgtct
     2641 tctttagttt ttgcaaaata gacgtgaccg ccgttttgcg tcactttttc tgagagctgg
     2701 tacagataag cgtcgagatt acttagaaca tgatcacgta tctgggcggc ccgatcgcgc
     2761 cactcctccc agtgccccaa ttcatcgacc attttttgcc gatttgcccc aatacgctgc
     2821 tgcgcgtttg ccaccgcttt gcgcatgatc ggatcttcaa tttgctgacg gatgcgtgtc
     2881 ttaaaatctg tattactggt tttgatcgac atctttatat cctcagcggc tcatcaacac
     2941 ttcagcaata tgcatcactt tgactttctg cccttcccgt tgtaatcgcc cactgatgtt
     3001 cagcaggcaa ctcacgtcag caccaattaa atactcaggg cggacttcca tcaggtgcac
     3061 aaccttttct ttcaccatct cgccggatat ttcggccatt ttgaccgaga acgtgccgcc
     3121 aaatccgcag caggtatcct gttcagcaaa ggtcaacagc tccagtccac gcacattttt
     3181 cagcagcgta agtggctcgt ccttcactcc cagcttacgg gccaggctac aagatgggtg
     3241 atacacagct ctcccttgca aactggcacc tacatcgact acccctaatt tattaacaat
     3301 aaaagaggtg agatcctgca tacgcgcggc aaccttttcg gcacgtgatg cccattcagg
     3361 ttcatccgcc agatacgtcg ggtaactttt tacggcatag gtgcaagagc cagccggtga
     3421 aataatggga tcgtcgttat cctccagtgc ggcgatcaga tttttcatcc ctggaatcgc
     3481 ttctttgata taaccgctat tgatcgcagg ctgaccgcag catccctgtt tctccgggaa
     3541 atttacgcga cagccgagtt tttccagtag cagcacggag tctcgtgcca ttcttgattt
     3601 cagggcgtca ccaatacagg tgacaaagaa attgacattc acaactaata ctccattact
     3661 tcatgcccat ttatgcccga tgtttccccc ccaccggggg atcaatcatc aataaatatg
     3721 gcgttctttt ttaattggct agcaacatac cacccctcat ttgtatgaca aatgataatc
     3781 tttcatccaa acttcaaatg caataactaa ttttatttct ttcggcatcg ctcacatttt
     3841 atggggaaat cgacgttcat ttttggcata agaaatccat ttaaaaaggc tacatccctt
     3901 atacctattc agagctactt attc
//
LOCUS       DAZKHF020000317         3905 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2201S31241
            SAMN43267800-rid25106413.denovo.319, whole genome shotgun sequence.
ACCESSION   DAZKHF020000317 DAZKHF020000000
VERSION     DAZKHF020000317.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267800
            Sequence Read Archive: SRR30307263
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3905)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3905)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 3905)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350581.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350581.2
            Genome Coverage       :: 151x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:21:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,652
            CDSs (total)                      :: 5,557
            Genes (coding)                    :: 5,320
            CDSs (with protein)               :: 5,320
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 237
            CDSs (without protein)            :: 237
            Pseudo Genes (ambiguous residues) :: 0 of 237
            Pseudo Genes (frameshifted)       :: 102 of 237
            Pseudo Genes (incomplete)         :: 130 of 237
            Pseudo Genes (internal stop)      :: 52 of 237
            Pseudo Genes (multiple problems)  :: 41 of 237
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3905
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267800-rid25106413.denovo.319"
                     /serotype="O 26:H11"
                     /isolate="2201S31241"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-01"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            complement(<1..240)
                     /gene="escO"
                     /locus_tag="ACHOSC_RS22085"
                     /old_locus_tag="pgaptmp_004417"
     CDS             complement(<1..240)
                     /gene="escO"
                     /locus_tag="ACHOSC_RS22085"
                     /old_locus_tag="pgaptmp_004417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312594.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system LEE ATPase activity
                     positive regulator EscO"
                     /protein_id="HGU4550217.1"
                     /translation="MAKINSQIKEVDGKLDDCEQAIKESIASKQAYCASLVNLDKVSL
                     YKYQIKNNAFDEQKQRLYEKKSSLSKEKRSLLDSQK"
     gene            complement(300..1640)
                     /gene="escN"
                     /locus_tag="ACHOSC_RS22090"
                     /old_locus_tag="pgaptmp_004418"
     CDS             complement(300..1640)
                     /gene="escN"
                     /locus_tag="ACHOSC_RS22090"
                     /old_locus_tag="pgaptmp_004418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system LEE ATPase EscN"
                     /protein_id="HGU4550218.1"
                     /translation="MILEHDSVLERYPRIQKVLNSTVPTLSLNSSTRYEGKITNIGGT
                     IIKARLPKARIGAFYKIEPSQRLAEVIAIDEDEVFLLPFEHISGMYCGQWLSYQGEEF
                     KIRVGDELLGRLVDGIGRPMGSNITAPYLPFERSLYAEPPDPLLRQVIDQPFTLGVRA
                     IDGLLTCGIGQRIGIFAGSGVGKSTLLGMICNGASADIIVLALIGERGREVNEFLALL
                     PQSTLSKCVLVVTTSDRPALERMKAAFTATTIAEFFRDQGKNVLLMMDSVTRYARAAR
                     DVGLASGEPDVRGGFPPSVFSSLPKLLERAGPAPKGSITAIYTVLLESDNVNDPIGDE
                     VRSILDGHIVLTRELAEENHFPAIDIGLSASRVMHNVVKSEHLRAAAECKKLIATYKN
                     IELLIRIGEYTMGQDPEADKAIKNRKAIQSFIQQSTKDISSYEKTIESLFKVVA"
     gene            complement(1624..3651)
                     /gene="escV"
                     /locus_tag="ACHOSC_RS22095"
                     /old_locus_tag="pgaptmp_004419"
     CDS             complement(1624..3651)
                     /gene="escV"
                     /locus_tag="ACHOSC_RS22095"
                     /old_locus_tag="pgaptmp_004419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system LEE export apparatus
                     protein EscV"
                     /protein_id="HGU4550219.1"
                     /translation="MNKLLNIFKKAESYHDLILALFFFMAVMMMIIPLPTVVVDIIIA
                     INISTALLLLMLSIYIKNPLELTSFPTILLITTLMRLSLSVSTTRLILLHHDAGDIIY
                     SFGNFVVGGNIVVGLVIFTIITIVQFMVITKGAERVAEVSARFSLDGMPGKQMSIDGD
                     MRAGVIDPLEAKVLRSRVQKESQFYGSMDGAMKFVKGDAIAGIIIVLVNLFGGVLIGM
                     WQFDMPFSEALSLFSVLSVGDALVAQIPALIISVTAGVVVTRVPGESEKEENLAGDIV
                     QQVSVNSRPFLISAALMIVMAIIPGFPTLVFLFLAVCLLGIAWKLQKKRTFGTGNNKD
                     AMGADLSNSQNISPGAEPLILNLSSNIYSSDITQQIEVMRWNFFEESGIPLPKIIVNP
                     VKNNDSAIEFLLYQESIYKDTLTDDTVYFEAGHAEISFEFVQEKLSANSIVYKTNEAN
                     QQLAHVSGMDVYAKTNDKITFLLKKLVLSNAKEFIGVQETRYLMDIMERKYNELVKEL
                     QRQLGLSKIVDILQRLVEENISIRDLRTIFETLIFWSTKEKDVVILCEYVRIALRRHI
                     LGRYSVRGTLLNVWLIGSDIENELRESIRQTSSGSYLNISPERSEQIIGFLKNIVNPT
                     GNGVILTALDIRRYVKKMIEGSFPSVPVLSFQEVGNNIELKVLGTVNDFRA"
     gene            complement(3648..>3905)
                     /locus_tag="ACHOSC_RS22100"
                     /old_locus_tag="pgaptmp_004420"
     CDS             complement(3648..>3905)
                     /locus_tag="ACHOSC_RS22100"
                     /old_locus_tag="pgaptmp_004420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system, LEE associated
                     protein"
                     /protein_id="HGU4550220.1"
                     /translation="GFSFFIEIIDSNVFITFALEYNENAFFSFFNALAPERTQGVIEH
                     IFVYDNKLCLSCLLTNIDVFSLMNTFQQHVQIIERVRRMTS"
BASE COUNT         1207 a          906 c          710 g         1082 t
ORIGIN      
        1 tttttgtgaa tcgagcaatg aacgcttttc tttagaaagt gaggattttt tctcatataa
       61 tcgctgcttt tgctcatcaa atgcattgtt tttaatctgg tatttataca acgacacttt
      121 atccagattc accagcgatg cacaatacgc ctgcttagag gcaatcgact ctttaatggc
      181 ttgctcgcag tcatcgagtt taccatccac ctcttttatc tgagagttaa tcttagccat
      241 tgattctctc aatcgattcg ctctgctttt acgaatagat aaaattctgt ccaacatatt
      301 caggcaacca ctttgaatag gctttcaatc gttttttcgt aactactgat atctttggtg
      361 ctttgttgta taaagctctg aattgctttc ctgtttttta ttgccttatc cgcttcagga
      421 tcttgcccca tcgtgtactc accaatacga ataagcagtt caatattctt ataggttgca
      481 ataagctttt tgcattcagc cgctgcacgt aaatgttccg atttaacaac gttatgcata
      541 acacgactgg cgcttaaacc aatatcaatt gcagggaaat ggttttcctc tgcaagttct
      601 cgggtaagca caatatgtcc atcaagaata gagcgaactt catcaccgat gggatcattc
      661 acattatcgc tttctaacag caccgtataa atcgcagtta ttgaaccttt tggcgcaggt
      721 cccgcacgct ctaataattt gggtaacgag gaaaaaacac tcggagggaa tccccctctt
      781 acatcaggct ctcccgatgc aagcccaaca tcacgcgcgg ctcgggcata acgagttacg
      841 gaatccatca ttaataatac atttttgccc tgatcccgga agaactctgc aatggttgtc
      901 gccgtaaatg cggccttcat tctttccagc gcgggacggt ctgacgttgt gacgaccagt
      961 acacatttag aaagcgtgga ttgaggtaaa agcgcgagga attcatttac ttcgcgacca
     1021 cgctcgccga taagggcaag gacaataata tccgcggatg cgccattaca aatcattccc
     1081 aaaagcgtac ttttaccaac gccggacccc gcgaaaatac cgatgcgttg cccaataccg
     1141 caagtaagta aaccatctat tgccctgacg ccaagcgtaa atggctgatc aataacctgc
     1201 cttaataaag gatcgggggg ttcagcatac aaactgcgtt caaacggaag ataaggtgca
     1261 gtaatattgc tccccattgg tctgcctata ccatcaacca gtcgcccaag gagttcgtcc
     1321 ccaacacgaa tcttgaactc ttccccctga taacttagcc actggccaca atacatgccg
     1381 gaaatatgct caaaaggcag caggaaaacc tcatcttcat caatagcgat cacctcagct
     1441 aaacgttgac tgggttctat cttgtaaaag gcaccaatac gcgcttttgg taaacgcgcc
     1501 ttgataatcg tcccgccgat attggtaatc ttgccttcat atctggtaga cgaatttaat
     1561 gataatgtcg gcacagtgct attgagcact ttctggatac gtgggtatct ttccaataca
     1621 gaatcatgct ctaaaatcat ttaccgttcc taatacttta agttcgatat tattcccaac
     1681 ttcctgaaaa gagagcacgg ggactgacgg gaacgaacct tcaatcattt tcttcacata
     1741 gcgcctgata tctaaagcgg tcagaatgac gccattcccc gttggattca caatattttt
     1801 taagaagcca ataatctgct cacttcgctc cggtgagata ttcaaatacg aacctgatga
     1861 cgtttgtctg atcgactctc gtaactcatt ttcaatatca gagccaataa gccagacatt
     1921 cagaagtgta ccgcgaacgc tatagcgacc taaaatatgc cgacggaggg cgatacgaac
     1981 gtattcgcac aagataacca catctttttc ttttgtggac caaaaaataa gcgtttcaaa
     2041 aatagttcgt agatctctaa ttgagatatt ttcctctacg agacgctgaa gaatatccac
     2101 aatcttgctc agaccaagct gacgctgaag ctctttcaca agttcgttat atttcctctc
     2161 catgatgtcc atcagataac gcgtttcttg tacgccgatg aactctttgg cattagacaa
     2221 tacaagtttc tttagcaaaa acgttatctt gtcatttgtt tttgcataaa catccatacc
     2281 tgagacgtga gctagttgtt ggttagcctc attcgtttta tacacgatag agttagccga
     2341 aagcttttcc tggacaaatt caaatgatat ctctgcatgc ccagcctcaa aataaacagt
     2401 atcatctgta agagtatctt tgtatattga ctcttgatag agcaaaaatt ctatagcgct
     2461 atcattattt ttaactggat taacaataat cttaggcaat ggaattccac tttcctcaaa
     2521 gaaattccaa cgcataacct caatctgctg tgtaatatct gagctataaa tattactact
     2581 taagtttaaa atcaacggct cggcacccgg tgagatattt tgactattag acaaatcagc
     2641 tcccatagcg tccttattat ttccggttcc aaacgttctt ttcttttgta atttccaggc
     2701 gatacccaac aggcaaaccg ccagaaacaa gaagaccaat gtaggaaagc caggaataat
     2761 cgccattacg atcatgagtg cggcactgat taaaaaaggg cgactattaa cagaaacctg
     2821 ttgaacaata tcacctgcaa ggttttcttc tttttcgctt tcaccgggaa cacgagtaac
     2881 aacaaccccc gcagtgacgg aaataataag cgcagggatc tgggcaacca aagcatcacc
     2941 gacagacaat acagaaaaca ggctaagtgc ttcactaaat ggcatgtcaa attgccacat
     3001 tccaatgagc acgccaccaa acaggttaac taaaacaatg atgataccgg caatagcgtc
     3061 cccttttaca aacttcatcg ccccgtccat tgaaccataa aactggcttt ccttttgcac
     3121 tcgggagcga agaacttttg cctcaagcgg atcaataacg cctgcgcgca tatcaccatc
     3181 aatactcatt tgttttcctg gcataccatc aagggaaaaa cgagcactca cttcagcaac
     3241 acgctcagcc ccttttgtaa taaccataaa ttgtacaatg gtgataatgg taaaaataac
     3301 caggccaaca acaatattac cgccaacaac aaagttaccg aaggagtaaa taatgtcacc
     3361 cgcatcgtga tgcagcagaa taagtctggt tgtactaaca ctgagcgaca ggcgcatcaa
     3421 cgtcgtaatc aacaggattg taggaaaaga agttagctca agaggatttt ttatatagat
     3481 tgagagcatt aataaaagca acgctgtcga tatattaatc gcgataatga tatccaccac
     3541 aactgttggc aatggaataa tcatcatcat tacagccata aagaagaaga gagccagaat
     3601 aagatcgtga tatgactctg cttttttaaa tatatttaag agtttattca tgatgtcatc
     3661 ctgcgaactc gttcaataat ctgcacatgc tgctgaaaag tattcattag agaaaacaca
     3721 tcaatattgg ttaacaaaca acttaagcac aatttgttat cgtagacaaa aatatgttca
     3781 atcactccct gcgtccgttc aggggcaagg gcattgaaaa aagaaaaaaa cgcattttcg
     3841 ttatactcta aggcaaacgt aataaacaca ttactatcaa ttatttcaat aaaaaaagaa
     3901 aaacc
//
LOCUS       DAZKHF020000318         3886 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2201S31241
            SAMN43267800-rid25106413.denovo.320, whole genome shotgun sequence.
ACCESSION   DAZKHF020000318 DAZKHF020000000
VERSION     DAZKHF020000318.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267800
            Sequence Read Archive: SRR30307263
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3886)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3886)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 3886)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350581.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350581.2
            Genome Coverage       :: 151x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:21:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,652
            CDSs (total)                      :: 5,557
            Genes (coding)                    :: 5,320
            CDSs (with protein)               :: 5,320
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 237
            CDSs (without protein)            :: 237
            Pseudo Genes (ambiguous residues) :: 0 of 237
            Pseudo Genes (frameshifted)       :: 102 of 237
            Pseudo Genes (incomplete)         :: 130 of 237
            Pseudo Genes (internal stop)      :: 52 of 237
            Pseudo Genes (multiple problems)  :: 41 of 237
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3886
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267800-rid25106413.denovo.320"
                     /serotype="O 26:H11"
                     /isolate="2201S31241"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-01"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            135..833
                     /locus_tag="ACHOSC_RS22105"
                     /old_locus_tag="pgaptmp_004421"
     CDS             135..833
                     /locus_tag="ACHOSC_RS22105"
                     /old_locus_tag="pgaptmp_004421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000577178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HGU4550221.1"
                     /translation="MIFQKIARLLKSEINGNSWHVGDLLPSEAELAVRYNVSRNTLRK
                     ALSLLEGEGIIHRKHGSGRYIQKKNFVAHIDHMNSFSEIAHKSGKEAGSQIMKFEVQD
                     ASPTIATELNLVTGEQVYYIKRLRFIEDNAAQLEETWMSVARFPDLTVSHMQKSKFSY
                     IENECGIKIIGTFETFSPTFPTPEIASILRISPRDPILKIQTQAVDSNSIPLDYSLLY
                     SNIFEFQVKYFFPR"
     gene            complement(878..1777)
                     /gene="eamA"
                     /locus_tag="ACHOSC_RS22110"
                     /old_locus_tag="pgaptmp_004422"
     CDS             complement(878..1777)
                     /gene="eamA"
                     /locus_tag="ACHOSC_RS22110"
                     /old_locus_tag="pgaptmp_004422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="O-acetylserine/cysteine exporter"
                     /protein_id="HGU4550222.1"
                     /translation="MSRKDGVLALLVVVVWGLNFVVIKVGLHNMPPLMLAGLRFMLVA
                     FPAIFFVARPKVPLNLLLGYGLTISFAQFAFLFCAINFGMPAGLASLVLQAQAFFTIV
                     LGAFTFGERLHGKQLAGIALAIFGVLVLIEDSLNGQHVAMLGFMLTLAAAFSWACGNI
                     FNKKIMSHSTRPAVMSLVIWSALIPIIPFFVASLILDGSASMIHSLVTIDMTTILSLM
                     YLAFVATIVGYGIWGTLLGRYETWRVAPLSLLVPVVGLASAALLLDERLTGLQFLGAV
                     LIMTGLYINVFGLWWRKAVKVRG"
     gene            1972..3159
                     /gene="ydeE"
                     /locus_tag="ACHOSC_RS22115"
                     /old_locus_tag="pgaptmp_004423"
     CDS             1972..3159
                     /gene="ydeE"
                     /locus_tag="ACHOSC_RS22115"
                     /old_locus_tag="pgaptmp_004423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux MFS transporter YdeE"
                     /protein_id="HGU4550223.1"
                     /translation="MNLSLRRSTSALLASSLLLTIGRGATLPFMTIYLSRQYSLSVDL
                     IGYAMTIALTIGVVFSLGFGILADKFDKKRYMLLAITAFASGFIAIPLVNNVTLVVLF
                     FALINCAYSVFATVLKAWFADNLSSTSKTKIFSINYTMLNIGWTIGPPLGTLLVMQSI
                     NLPFWLAAICSAFPMLFIQIWVKRSEKIIATETGSVWSPKVLLQDKALLWFTCSGFLA
                     SFVSGAFASCISQYVMVIADGDFAEKVVAVVLPVNAAMVVTLQYSVGRRLNPVNIRAL
                     MTAGTLCFVIGLVGFIFSGNSLLLWGMSAAVFTVGEIIYAPGEYMLIDHIAPPGMKAS
                     YFSAQSLGWLGAAINPLVSGVVLTSLPPSSLFIILALVIIAAWVLMLKGIRAKPWGQP
                     ALC"
     gene            3286..3381
                     /gene="mgtS"
                     /locus_tag="ACHOSC_RS22120"
                     /old_locus_tag="pgaptmp_004424"
     CDS             3286..3381
                     /gene="mgtS"
                     /locus_tag="ACHOSC_RS22120"
                     /old_locus_tag="pgaptmp_004424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein MgtS"
                     /protein_id="HGU4550224.1"
                     /translation="MLGNMNVFMAVLGIILFSGFLAAYFSHKWDD"
     gene            3381..3485
                     /gene="mgtT"
                     /locus_tag="ACHOSC_RS22125"
                     /old_locus_tag="pgaptmp_004425"
     CDS             3381..3485
                     /gene="mgtT"
                     /locus_tag="ACHOSC_RS22125"
                     /old_locus_tag="pgaptmp_004425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein MgtT"
                     /protein_id="HGU4550225.1"
                     /translation="MNGDNPSPNRPLVTVVYKGPDFYDGEKKPPVNRR"
     gene            complement(3600..>3886)
                     /gene="dgcZ"
                     /locus_tag="ACHOSC_RS22130"
                     /old_locus_tag="pgaptmp_004426"
     CDS             complement(3600..>3886)
                     /gene="dgcZ"
                     /locus_tag="ACHOSC_RS22130"
                     /old_locus_tag="pgaptmp_004426"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="diguanylate cyclase DgcZ"
                     /protein_id="HGU4550226.1"
                     /translation="YRYGGEEFIIIVKAANDEEACRAGVRICQLVDNHAITHSEGHIN
                     ITVTAGVSRAFPEEPLDVVIGRADRAMYEGKQTGRNRCMFIDEQNVINRV"
BASE COUNT         1042 a          933 c          845 g         1066 t
ORIGIN      
        1 gtcctggtac attgatcaca gtaatacagt gataaagttg ttcaacatca gggtgattat
       61 taaacgactc ggccatacga taaaccaatc aggactcaac tccttgtttt caatggctct
      121 taaaaggtgc aaaagtgatt tttcaaaaga ttgcccgctt acttaaatcc gaaatcaatg
      181 gcaattcatg gcatgttggt gatttgttgc cgtcagaagc ggaactcgct gttcgttata
      241 atgtttcgcg taataccctg cgtaaggcat tatccttgct ggaaggcgaa ggaattattc
      301 acagaaagca tggttcagga agatacattc aaaaaaagaa ttttgttgca cacattgatc
      361 acatgaacag tttcagtgaa attgcgcata aaagcggcaa agaggcaggg agtcagatta
      421 tgaaatttga agtgcaggat gcttcaccca ctattgcgac tgagctgaat ttagtgactg
      481 gtgagcaggt ttattacata aagagactgc gattcattga ggataatgca gcacaattag
      541 aagaaacgtg gatgtcagta gcacgttttc ctgatttaac cgtgtcacat atgcaaaaat
      601 ccaaattttc atatattgag aatgaatgcg ggatcaaaat cattggcacc tttgaaactt
      661 tctcccctac ttttcctacc ccagaaatcg ccagtatttt acggatcagc ccacgggatc
      721 ccatacttaa aattcagacc caggctgtgg acagtaactc tattccgctg gattattcgt
      781 tactttacag caatattttc gagttccagg taaagtactt tttcccgcga taaaagcaga
      841 cgcacagagt gctgacatca cattctgtgc gtatggttta gcctctaacc tttaccgctt
      901 tacgccacca caagccaaat acattgatat acagcccggt cataatgagc accgcaccta
      961 aaaattgcag acccgttaag cgttcatcca acaatagtgc cgcacttgcc agtccgacta
     1021 cgggcaccag taacgataac ggtgcaaccc gccaggtttc atagcgtccc agtaacgtcc
     1081 cccagatccc ataaccaaca attgtcgcca caaacgccag atacatcagc gacaagatgg
     1141 tggtcatatc aatagtaacc agactgtgaa tcatggatgc ggaaccatcg agaatcagcg
     1201 aggcaacaaa gaagggaatg attgggatta aagcgctcca gattaccagc gacatcaccg
     1261 ccggacgcgt tgagtgcgac atgatctttt tattgaagat gttgccacac gcccaactaa
     1321 atgctgcagc cagggtcaac ataaagccga gcatcgccac atgctgaccg ttcagactat
     1381 cttcgattaa caccagtacg ccaaaaatcg ctaaggcgat ccccgccaat tgtttgccat
     1441 gcagtcgctc cccgaaagta aacgcgccaa gcacgatagt aaaaaacgcc tgtgcctgta
     1501 acaccagcga agccagtcca gcaggcatac caaagttaat ggcgcagaac agaaaggcaa
     1561 actgcgcaaa actgatggtt aatccatacc ccaagagcaa attcagtggt actttcggtc
     1621 gtgcaacaaa aaagatggcc ggaaaagcga ccagcataaa gcgcaaaccg gccagcatca
     1681 gcggtggcat gttatgaagc cccactttga tgaccacaaa atttagcccc catacgacca
     1741 ctaccagtag cgccaacacc ccatcttttc gcgacattct accgcctctg aatttcatct
     1801 tttgtaagca atcaacttag ctgaatttac ttttctttaa cagttgattc gttagtcgcc
     1861 ggttacgacg gcattaatgc gcaaataagt cgctatactt cggatttttg ccatgctatt
     1921 tctttacatc tctaaaacaa aacataacga aacgcactgc cggacagaca aatgaactta
     1981 tccctacgac gctctaccag cgcccttctt gcctcgtcgt tgttattaac catcggacgc
     2041 ggcgctacgc tgccatttat gaccatttac ttgagtcgcc agtacagcct gagtgtcgat
     2101 ctaatcggtt atgcgatgac aattgcgctc actattggcg tcgtttttag cctcggtttt
     2161 ggtatcctgg cggataagtt cgacaagaaa cgctatatgt tactggcaat taccgccttc
     2221 gccagcggtt ttattgccat tcctttagtg aataacgtga cgctggtggt gctctttttt
     2281 gcccttatta actgcgccta ttccgttttt gctaccgtgt tgaaagcctg gtttgccgac
     2341 aatctttcgt ccaccagcaa aacgaaaatc ttctcaatca actacaccat gctaaacatt
     2401 ggctggacca tcggtccgcc gctcggcacg ctgttggtga tgcagagcat caatctgccc
     2461 ttttggctgg cagctatctg ttccgcgttt cccatgcttt tcattcaaat ttgggtaaag
     2521 cgcagcgaga aaatcatcgc cacggaaaca ggcagtgtct ggtcgccgaa agttttatta
     2581 caagataaag cactgttgtg gtttacctgc tctggttttc tggcttcctt tgttagcggc
     2641 gcatttgctt catgcatttc acaatatgtg atggtgattg ctgatggaga ttttgccgaa
     2701 aaggtggtcg cggttgttct tccggttaat gctgccatgg tagttacgtt gcaatattcc
     2761 gtgggccgcc gccttaaccc ggttaacatc cgcgcgctga tgacagcagg caccctctgt
     2821 ttcgtcatcg gtctggttgg ttttattttt tccggcaaca gcctgctatt gtggggtatg
     2881 tcagctgcgg tatttactgt cggtgaaatc atttatgcgc cgggcgagta tatgttgatt
     2941 gaccatattg cgccgccagg aatgaaagcc agctattttt ctgcccagtc tttaggctgg
     3001 cttggtgctg caattaaccc attagtgagt ggcgtagtgc taaccagcct gccgccttcc
     3061 tcgctgttta tcatcttagc gttggtgatc attgctgcgt gggtgctgat gttaaaaggg
     3121 attcgtgcaa aaccgtgggg gcagcccgcg ctttgttgat ttaagtcgaa cacaataaaa
     3181 atttaattca gccttcgttt aggttacctc tgctaatatc tttctcattg agatgaaaat
     3241 taaggtaagc gaggaaacac accacaccat aaacggaggc aaataatgct gggtaatatg
     3301 aatgttttta tggccgtact gggaataatt ttattttctg gttttctggc cgcgtatttc
     3361 agccacaaat gggatgacta atgaacggag ataatccctc acctaaccgg ccccttgtta
     3421 cagttgtgta caaggggcct gatttttatg acggcgaaaa aaaaccgcca gtaaaccggc
     3481 ggtgaatgct tgcatggata gatttgtgtt ttgcttttac gctaacaggc attttcctgc
     3541 actgataacg gatcgttgac acagtagcat cagttttctc aatgaatgtt aaacggagct
     3601 taaactcggt taatcacatt ttgttcgtca ataaacatgc agcgatttct tccggtttgc
     3661 ttaccctcat acattgcccg gtctgctctt ccaatgacca catccagagg ctcttcagga
     3721 aatgcgcgac tcacacctgc tgtcacggta atgttgatat gcccttcaga atgtgtgatg
     3781 gcatggttat cgactaactg gcaaattctg acacctgcac gacatgcttc ttcatcatta
     3841 gccgctttga caataatgat aaattcttcg cccccgtagc gataaa
//
LOCUS       DAZKHF020000319         3878 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2201S31241
            SAMN43267800-rid25106413.denovo.321, whole genome shotgun sequence.
ACCESSION   DAZKHF020000319 DAZKHF020000000
VERSION     DAZKHF020000319.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267800
            Sequence Read Archive: SRR30307263
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3878)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3878)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 3878)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350581.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350581.2
            Genome Coverage       :: 151x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:21:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,652
            CDSs (total)                      :: 5,557
            Genes (coding)                    :: 5,320
            CDSs (with protein)               :: 5,320
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 237
            CDSs (without protein)            :: 237
            Pseudo Genes (ambiguous residues) :: 0 of 237
            Pseudo Genes (frameshifted)       :: 102 of 237
            Pseudo Genes (incomplete)         :: 130 of 237
            Pseudo Genes (internal stop)      :: 52 of 237
            Pseudo Genes (multiple problems)  :: 41 of 237
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3878
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267800-rid25106413.denovo.321"
                     /serotype="O 26:H11"
                     /isolate="2201S31241"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-01"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            <1..206
                     /locus_tag="ACHOSC_RS22135"
                     /old_locus_tag="pgaptmp_004427"
     CDS             <1..206
                     /locus_tag="ACHOSC_RS22135"
                     /old_locus_tag="pgaptmp_004427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000440209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="HGU4550227.1"
                     /translation="YLKPYGVLDSKGFGVFLAMICATFIISIISYNLIEKPSVSIGHA
                     IARRMQKKSDVSSIKTESVREAP"
     gene            complement(451..1608)
                     /gene="intS"
                     /locus_tag="ACHOSC_RS22140"
                     /old_locus_tag="pgaptmp_004428"
     CDS             complement(451..1608)
                     /gene="intS"
                     /locus_tag="ACHOSC_RS22140"
                     /old_locus_tag="pgaptmp_004428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prophage integrase IntS"
                     /protein_id="HGU4550228.1"
                     /translation="MLTVKQIEAAKPKEKPYRLLDGNGLYLYVPVSGKKVWQLRYKID
                     GKEKILTVGKYPLMTLQEARDKAWTARKDISVGIDPVKAKKASSNNNSFSAIYKEWYE
                     HKKQVWSVGYATELAKMFDDDILPIIGGLEIQDIEPMQLLEVIRRFEDRGAMERANKA
                     RRRCGEVFRYAIVTGRAKYNPAPDLADAMRGYRKKNFPFLPADQIPAFNKALATFSGS
                     IVSLIATKVLRYTALRTKELRSMQWKNVDFENRIITIDANVMKGRKIHVVPMSDQVVE
                     LLTTLSSITKPVSEFVFAGRNDKKKPICENAVLLVIKQIGYEGLESGHGFRHEFSTIM
                     NEHEWPADAIEVQLAHANGGSVRGIYNHAQYLDKRREMMQWWADWLDEKVE"
     gene            complement(1770..1844)
                     /locus_tag="ACHOSC_RS22145"
                     /old_locus_tag="pgaptmp_004429"
     tRNA            complement(1770..1844)
                     /locus_tag="ACHOSC_RS22145"
                     /old_locus_tag="pgaptmp_004429"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1809..1811),aa:Arg,seq:cct)
     gene            complement(1920..2852)
                     /gene="yfdC"
                     /locus_tag="ACHOSC_RS22150"
                     /old_locus_tag="pgaptmp_004430"
     CDS             complement(1920..2852)
                     /gene="yfdC"
                     /locus_tag="ACHOSC_RS22150"
                     /old_locus_tag="pgaptmp_004430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015499 - formate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015724 - formate transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate/nitrite transporter family protein"
                     /protein_id="HGU4550229.1"
                     /translation="MDNDKIDQHSDEIEVESEEKERGKKIEIDEDRLPSRAMAIHEHI
                     RQDGEKELERDAMALLWSAIAAGLSMGASLLAKGIFHVELEGVPGSFLLENLGYTFGF
                     IIVIMARQQLFTENTVTAVLPVMQKPTMSNVGLLMRLWGVVLLGNILGTGIAAWAFEY
                     MPIFNEETRDAFVKIGMDVMKNTPSEMFANAIISGWLIATMVWMFPAAGAAKIVVIIL
                     MTWLIALGDTTHIVVGSVEILYLVFNGTLHWSDFIWPFALPTLAGNICGGTFIFALMS
                     HAQIRNDMSNKRKAEARQKAERAENIKKNDKNPA"
     gene            3146..>3878
                     /gene="mlaA"
                     /locus_tag="ACHOSC_RS22155"
                     /old_locus_tag="pgaptmp_004431"
     CDS             3146..>3878
                     /gene="mlaA"
                     /locus_tag="ACHOSC_RS22155"
                     /old_locus_tag="pgaptmp_004431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipid-binding lipoprotein MlaA"
                     /protein_id="HGU4550230.1"
                     /translation="MKLRLSALALGTTLLVGCASSGTDQQGRSDPLEGFNRTMYNFNF
                     NVLDPYIVRPVAVAWRDYVPQPARNGLSNFTGNLEEPAVMVNYFLQGDPYQGMVHFTR
                     FFLNTILGMGGFIDVAGMANPKLQRTEPHRFGSTLGHYGVGYGPYVQLPFYGSFTLRD
                     DGGDMADGLYPVLSWLTWPMSVGKWTLEGIETRAQLLDSDGLLRQSSDPYIMVREAYF
                     QRHDFIANGGELKPQENPNAQAIQDD"
BASE COUNT          932 a          935 c          924 g         1087 t
ORIGIN      
        1 tatatctcaa accgtacggg gtattggata gtaaaggatt tggtgttttt ctagctatga
       61 tttgcgctac ttttattata tcaatcatat cttataacct tattgaaaaa ccatcagtat
      121 ccatagggca tgccatcgct cgtagaatgc aaaaaaaatc agatgtatct tcaattaaaa
      181 ctgaatccgt tagagaagca ccttgataaa tagagcaata gccagatagc acctaaccga
      241 tttcgtactc atagggttgt cactttacca aactgagagg aaatacactg ataacagttg
      301 gttatgaagt actgggtgag gtggatgtga atggtatttt gatgcatttc attaacctgt
      361 acgcctaaga aagcaaccct gaaatctaag acacacaagc tttgcactgg attgcaaggc
      421 tttgtgcact tcgatagtgg ttaaggtggt tcactccacc ttttcatcaa gccagtccgc
      481 ccaccattgc atcatttctc tgcgcttatc gagatactga gcatggttgt aaataccgcg
      541 cacagatccg ccgttggcat gtgccagttg cacttcaata gcgtcagcag gccattcgtg
      601 ctcgttcata atcgtgctga attcatgcct gaatccgtga ccgctttcca gaccctcata
      661 gccgatttgt ttgatcacaa gtagcaccgc gttctcgcag attggcttct tcttatcgtt
      721 gcgcccggca aaaacaaatt ctgatactgg tttggtgatg gagcttaacg tagtgagaag
      781 ttcaaccacc tggtccgaca tcgggacaac atgaattttg cgtcccttca tcacattggc
      841 gtcaatggtg ataatcctgt tttcaaaatc gacgttcttc cattgcatgg aacgaagctc
      901 ttttgttctt agggctgtgt agcgtaaaac cttggtggca atgagcgata cgatacttcc
      961 tgaaaatgtt gccagtgctt tattgaatgc cgggatctgg tctgcaggaa gaaacgggaa
     1021 gttcttcttg cggtatcccc tcatggcgtc agcaaggtca ggtgccgggt tatatttagc
     1081 cctgccggtg acaatagcgt aacggaaaac ctcgccgcat cttctgcggg ctttatttgc
     1141 tcgctccatt gcaccgcgat cttcaaatct gcggattact tccagcagtt gcatcggctc
     1201 aatatcctga atttcaaggc cgccgatgat aggcaaaatg tcgtcatcaa acatttttgc
     1261 aagttcagtc gcatacccta ctgaccatac ttgcttcttg tgctcgtacc attccttgta
     1321 aatcgcacta aaggaattgt tgttagacga agcctttttc gcctttaccg gatcgatgcc
     1381 aaccgagatg tctttcctcg cagtccatgc cttatccctt gcctcctgca aagtcattag
     1441 cggatatttt ccgacggtca ggattttctc cttaccgtca atcttgtagc gaagctgcca
     1501 tacctttttc ccggatacag gaacataaag gtacaggcca ttaccatcga gaaggcggta
     1561 tggtttttct ttcggctttg ctgcttcaat ctgcttaacg gtgagcatgg gtaaaaatcc
     1621 ggagggtaaa attattttat ccacttttta cccgtcatgg ggtgcggctg tcaacgatct
     1681 gacgcgaacc atgacgaact gtgaatctac ggaaggcttg atattcaggg gattttgcgg
     1741 actggtacgg atgggagcga actgataaat ggtgtcccct gcaggaatcg aacctgcaat
     1801 tagcccttag gaggggctcg ttatatccat ttaactaaga ggacaatgcg gcatgagtat
     1861 acccgctaat ggactgcggg gtaagtacgc tgccgctcga ttgcttaaac cctcgccatt
     1921 tatgctgggt ttttatcatt tttcttaatg ttttccgcac gttctgcttt ttggcgtgct
     1981 tctgctttac gcttgttgct catgtcgtta cgaatctgtg catgactcat taacgcgaag
     2041 ataaaggtgc cgccgcagat gttccccgct aaagtaggta gtgcgaaggg ccagatgaaa
     2101 tcgctccagt gcagcgtgcc gttaaacacc agatagagga tttcaacaga accgacaacg
     2161 atgtgggtgg tgtcacccag tgcaataagc caggtcatca aaataatcac cacaatcttt
     2221 gccgcacccg ctgcaggaaa catccaaacc atagtggcga tcagccagcc ggaaatgatc
     2281 gcgttggcaa acatctcgct gggggtgttc ttcatcacat ccatgccgat tttgacaaat
     2341 gcatcgcgag tttcttcatt gaagataggc atatattcaa atgcccatgc agcaatacct
     2401 gtcccgagaa tattacccag cagcacgacg ccccataatc gcataagtaa gccgacgttg
     2461 ctcattgtcg gtttttgcat gacgggtagt accgcagtca cggtgttttc ggtaaataat
     2521 tgctggcggg ccataatgac gataataaaa ccaaaggtat aaccgagatt ctccagtaag
     2581 aagctgcctg gcactccttc cagttcgaca tgaaatatcc cttttgccag tagcgaagcg
     2641 cccatcgaca gacccgccgc aatggctgac cacagtagcg ccattgcgtc gcgttccagc
     2701 tctttttcac catcctggcg gatatgctca tgaattgcca tcgcccggga ggggagtcgg
     2761 tcttcatcta tttctatttt tttgccgcgc tctttttctt cgctctcaac ttcaatttcg
     2821 tcgctgtgtt gatcaatttt gtcgttgtcc atggtctctt ctcttgaatt agcacgtata
     2881 gctaaagcgt agcggctttt ttgctcgcaa ctggcgggag ttactctgaa aatgtagaaa
     2941 aggctgcgtt tgcctttttc tgtttctata gaatcaagta gcctacaggg cggcgattac
     3001 caggctatga tcaaatcagc aaatcagggc gtctggacat cagttgacgt gctgttacaa
     3061 tcgcccacac ctaaacaggc ggatacggta tcgttccgtc atggatggca aactgcataa
     3121 gccataaaaa aacagggaga catttatgaa gcttcgcctg tcggcgcttg ctctgggaac
     3181 tacgcttctg gtggggtgtg cgagttccgg tacagatcag caagggcgtt ctgacccgtt
     3241 agaagggttc aaccgcacca tgtacaactt caacttcaac gtattagacc cgtatattgt
     3301 tcgaccggtc gctgtcgcct ggcgtgatta tgttccgcaa ccggcgcgta atggtttgag
     3361 caactttact ggcaaccttg aagaacctgc ggtgatggtt aactacttct tgcagggcga
     3421 cccttatcag gggatggtcc actttacccg ctttttcctg aacaccattt tggggatggg
     3481 cggctttatt gatgttgcag ggatggcgaa cccgaaactg caacggactg aacctcaccg
     3541 cttcggtagt acgcttggtc attatggcgt gggttatggg ccttacgttc agttaccgtt
     3601 ctacggtagc ttcacgctgc gtgatgacgg tggtgatatg gcggatggtc tttacccggt
     3661 tctttcctgg ctgacctggc cgatgtctgt gggtaaatgg acgcttgaag ggatcgaaac
     3721 ccgcgctcag ctgctggatt ccgatggtct gctgcgtcag tcgtccgatc cttatattat
     3781 ggtgcgcgaa gcgtacttcc agcgtcatga tttcatcgct aatggcggcg aactcaaacc
     3841 gcaggaaaat ccgaacgcac aagcgattca ggatgatt
//
LOCUS       DAZKHF020000320         3875 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2201S31241
            SAMN43267800-rid25106413.denovo.322, whole genome shotgun sequence.
ACCESSION   DAZKHF020000320 DAZKHF020000000
VERSION     DAZKHF020000320.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267800
            Sequence Read Archive: SRR30307263
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3875)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3875)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 3875)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350581.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350581.2
            Genome Coverage       :: 151x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:21:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,652
            CDSs (total)                      :: 5,557
            Genes (coding)                    :: 5,320
            CDSs (with protein)               :: 5,320
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 237
            CDSs (without protein)            :: 237
            Pseudo Genes (ambiguous residues) :: 0 of 237
            Pseudo Genes (frameshifted)       :: 102 of 237
            Pseudo Genes (incomplete)         :: 130 of 237
            Pseudo Genes (internal stop)      :: 52 of 237
            Pseudo Genes (multiple problems)  :: 41 of 237
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3875
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267800-rid25106413.denovo.322"
                     /serotype="O 26:H11"
                     /isolate="2201S31241"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-01"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            complement(65..262)
                     /locus_tag="ACHOSC_RS22160"
                     /old_locus_tag="pgaptmp_004432"
     CDS             complement(65..262)
                     /locus_tag="ACHOSC_RS22160"
                     /old_locus_tag="pgaptmp_004432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309806.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TrmB family transcriptional regulator"
                     /protein_id="HGU4550231.1"
                     /translation="MLKQQDMTETARVVFNELSVTEPATVGEIAQNTYLSRERCQLIL
                     TQLVMAGLADYQFGCYRRLPQ"
     gene            complement(494..1036)
                     /locus_tag="ACHOSC_RS22165"
                     /old_locus_tag="pgaptmp_004433"
     CDS             complement(494..1036)
                     /locus_tag="ACHOSC_RS22165"
                     /old_locus_tag="pgaptmp_004433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001502553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1133 family protein"
                     /protein_id="HGU4550232.1"
                     /translation="MIYPEITGKSGEHLRLKTLEAVWIQGKLRMWGRWSYIGGGKPGN
                     MFNQLLASKKLTKTAINEALRRIRESGIDKPELEAFLREMIAGRQKSWLSHCTDAEAL
                     RIDGVISKALARYPGLIDILRQRYEGRGMSKRKMAELLNEVHPEWCFSTCEKRIANWL
                     AVAEYALYIPMRESFAQKMS"
     gene            complement(1045..1410)
                     /locus_tag="ACHOSC_RS22170"
                     /old_locus_tag="pgaptmp_004434"
     CDS             complement(1045..1410)
                     /locus_tag="ACHOSC_RS22170"
                     /old_locus_tag="pgaptmp_004434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016238003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RusA family crossover junction
                     endodeoxyribonuclease"
                     /protein_id="HGU4550233.1"
                     /translation="MTERIEFVLPYPPTVNTYWRRRGSTYFVSKAGERYRRDVALIVR
                     QQRLKLNLSGRLAIKIIAEPPDKRRRDLDNILKAPLDALMHAGLLIDDEQFDEINIVR
                     GQRVPGGRLGVKIYKIESE"
     gene            complement(1411..2466)
                     /locus_tag="ACHOSC_RS22175"
                     /old_locus_tag="pgaptmp_004435"
     CDS             complement(1411..2466)
                     /locus_tag="ACHOSC_RS22175"
                     /old_locus_tag="pgaptmp_004435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001265086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF968 domain-containing protein"
                     /protein_id="HGU4550234.1"
                     /translation="MRVLLRPVPVPELGLVVLKPGRESMQVFHNPRVLVEPEPKSMRG
                     LPSGVVPAVRQPLAEDKSLLPFFSDERVIRAAGGAGALSDWLLRHIKSCQWPHGDYHH
                     SETVIHRYGTGAMVLCWHCDNQLRNQTSESLGQLAHQNLSAWMIDVIRHAMNGTQERE
                     LSLAELSWWATINNVADALPETVLRRSLGLRAEKIRSVYRESDIVPGEQTATSMLKQR
                     TKNIALLPHAHQQNPPQEKTVVSIAVDPESPAQYLQRQKPRREEMPVYTRWVKTQKCM
                     TCGNQADDPHHIIGHGLGGMGTKADDLFVIPLCRKCHSELHAGVKDFEEKHGSQLLLL
                     IRFLMHARNSGVLKWKA"
     gene            complement(2468..2740)
                     /locus_tag="ACHOSC_RS22180"
                     /old_locus_tag="pgaptmp_004436"
     CDS             complement(2468..2740)
                     /locus_tag="ACHOSC_RS22180"
                     /old_locus_tag="pgaptmp_004436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4550235.1"
                     /translation="MVHKTKFHNSDTDLAIRHGRRSHAFKTDWYQHPPCTEEQAEWLI
                     HNYRRRGYEIKKALSLDYRHWIIYVRLPYSERPPRPSRTFQQRIWR"
     gene            2687..2866
                     /locus_tag="ACHOSC_RS22185"
                     /old_locus_tag="pgaptmp_004437"
     CDS             2687..2866
                     /locus_tag="ACHOSC_RS22185"
                     /old_locus_tag="pgaptmp_004437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001112740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4550236.1"
                     /translation="MPDGKVGIRIVKFGFVHHLFSLLAQQVSGVQADVRIVNQNARKK
                     QNPPKRVKCGCVEDA"
     gene            complement(2908..3063)
                     /gene="hokD"
                     /locus_tag="ACHOSC_RS22190"
                     /old_locus_tag="pgaptmp_004438"
     CDS             complement(2908..3063)
                     /gene="hokD"
                     /locus_tag="ACHOSC_RS22190"
                     /old_locus_tag="pgaptmp_004438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin HokD"
                     /protein_id="HGU4550237.1"
                     /translation="MKQQKAMLIALIVICLTVIVTALVTRKDLCEVRIRTGQTEVAVF
                     TAYEPEE"
     gene            3621..>3875
                     /locus_tag="ACHOSC_RS22195"
                     /old_locus_tag="pgaptmp_004439"
     CDS             3621..>3875
                     /locus_tag="ACHOSC_RS22195"
                     /old_locus_tag="pgaptmp_004439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001721311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta family protein"
                     /protein_id="HGU4550238.1"
                     /translation="MTISYIPILKAKRSELSALSQLSIEKKSKILPLLEIEPVPIDPD
                     SGIALKSYNETLIEFGKKVSKSCSDMQGVYIDGLLIEEHFI"
BASE COUNT          958 a          994 c          866 g         1057 t
ORIGIN      
        1 agcagatggc tggcaggtgc cgctaacacc caccagccgc ccattaccac aaattaaaaa
       61 gccttcactg cggaaggcgt ctgtaacaac cgaactgata atctgccaga cccgccataa
      121 caagctgagt cagtattaac tggcagcgtt cgcgtgaaag gtaagtattc tgcgcaattt
      181 ccccgacggt cgccggttcg gtgacgctta attcattaaa caccactctg gcggtttcgg
      241 tcatatcctg ctgttttagc atgctttttt cccttttccg gttaacgtga cataccaata
      301 cctcttgtcg aaaaagccag caagctgaaa gaccagtatt cacaactacc agcgcgttta
      361 atgttctgtg ccgtttttca gacataaaaa aacccgcata aagcgggttc tttcaggtgt
      421 ccatgtctgc tattcgcctc gcggtatagc tttgcgaagc gtagctggat tgaaacagtt
      481 tatgcgtaaa aaatcaagac attttttgag caaacgattc tcgcataggg atgtatagcg
      541 catattcagc aacagccaac caattagcaa ttcgcttttc gcatgtacta aaacaccatt
      601 ccgggtgcac ctcattcaac aattcagcca ttttgcgttt actcatcccc cgcccttcgt
      661 acctttgccg caggatatca atcaatccag gataacgtgc aagcgcttta cttatcaccc
      721 catcaatgcg taacgcctct gcatcagtac agtgagacaa ccagctcttc tgtctgccag
      781 cgatcatctc tcgcaagaat gcttccagct ctggtttatc aatccctgac tccctgattc
      841 tacgcagggc ttcattgatt gcggtttttg tcagtttttt ggatgccagc aactgattga
      901 acatatttcc tggtttgcca ccacctatat acgaccaacg cccccacatc cgtaatttcc
      961 cctggatcca gacggcttcc agcgttttta gacgtaaatg ctcgccgctt ttgcctgtaa
     1021 tttccgggta tatcatattt acgatcactc actctcaatt ttgtaaatct tcacgcccag
     1081 ccgtccccca ggaacgcgct gaccgcgcac aatattgatt tcatcaaact gctcgtcgtc
     1141 tatgagaagt ccggcatgca tcagcgcatc cagtggtgct ttcaggatat tgtccaggtc
     1201 acgacgacgt ttatccggtg gctctgcaat aatctttatc gccagccttc cggacaggtt
     1261 taatttcagc cgctgctggc gaacaataag cgccacatca cggcgataac gctcaccggc
     1321 ttttgataca aaatatgtgc tgccacgacg tcgccagtaa gtgttcaccg ttggcgggta
     1381 aggcaaaaca aattctatgc gttcagtcat tcatgctttc cacttcagga cacccgaatt
     1441 tctcgcgtgc attaaaaaac gaatcagcaa caacagctgg ctgccgtgtt tttcttcaaa
     1501 atcttttacc ccggcgtgta gttcgctatg gcatttacgg cacagcggaa taacaaacaa
     1561 atcatcagcc tttgttccca tccctcccag tccatgacca atgatgtgat gcggatcatc
     1621 tgcctgatta ccacacgtca tgcatttctg cgtttttacc caacgcgtgt atacaggcat
     1681 ctcttcccgt cgtggtttct ggcgctggag atactgagcc ggtgactccg gatcaacggc
     1741 aatgcttacc accgtctttt cctgtggcgg gttttgctgg tgggcgtgag gcagtagcgc
     1801 aatatttttt gtgcgctgct tcagcatgct ggtggcggtc tgctctcccg gcacgatgtc
     1861 gctctcgcgg tatactgagc gaattttttc cgcgcgtaat cccagtgaac gacgtaacac
     1921 cgtctccggt agtgcgtccg ctacgttatt tatggttgcc caccaggata attcagccag
     1981 cgataattcc cgctcctgcg tgccattcat tgcgtggcgg atgacatcaa tcatccatgc
     2041 tgacaggttt tggtgagcaa gctgcccgag tgattcggaa gtctggttac gcagctggtt
     2101 gtcgcagtgc cagcacaaca ccatcgcgcc ggtaccgtaa cgatgtatga cggtttcact
     2161 gtgatggtag tcaccatgag gccactggca ggatttaatg tggcgtaaca gccagtcaga
     2221 caatgcacca gcaccaccag cagcacgaat cacccgctca tcgctgaaaa atggcagtaa
     2281 tgatttatcc tccgccagcg gctggcgaac agcaggaacg actccggacg gcagaccgcg
     2341 catgcttttc ggttccggct ccaccagaac tcgagggtta tgaaatacct gcatggattc
     2401 acggcccggt tttagcacca ccagcccaag ttccggtacc ggaacaggtc gaagtaatac
     2461 ccgcacgtta cctccagatg cgttgctgga atgtgcggga cggacgcggt gggcgttcgg
     2521 aataagggag cctgacatag attatccagt gacgataatc gaggctgagg gctttcttaa
     2581 tctcgtatcc gcgtctgcgg tagttatgaa ttagccattc ggcctgttct tcagtacatg
     2641 gtgggtgttg gtaccagtcg gttttaaatg cgtgtgaacg ccgcccatgc cggatggcaa
     2701 ggtcggtatc agaattgtga aatttggttt tgtgcaccat ctgttttctc tgctggcgca
     2761 gcaggtgtca ggtgttcagg ctgacgtgcg aattgtaaac cagaatgcca ggaaaaaaca
     2821 aaacccgccg aagcgggtta agtgcgggtg cgttgaggat gcctgactca tcagaggtgg
     2881 cgagggattt ctccctcgcc tggtctctta ctcctcaggt tcgtaagctg tgaagacagc
     2941 gacctccgtc tggccggttc ggattcgtac ctcgcagagg tctttcctcg ttaccagtgc
     3001 cgtcactatg acggttaaac agatgacgat cagggcgatt aacatcgcct tttgctgctt
     3061 catagcctgc ttctccttgc ctttcggcac gtaagaggct aacctacatg tgcaaagcat
     3121 gaaattggcc tcagattaat gttaagcgtc ttgccggacg cgtaatgtta actggggctt
     3181 ttctctatct gccttttggt gttcatgcct gaggcagata gcctcaagca cccgcagcaa
     3241 ttctaactat ccaatagatc aatgccaact tcttttcctg caacatctct catcagaagt
     3301 gacccaaatt catccaatca gcacaaaaga aacatttaaa gcgtgaatgc taccgaacgt
     3361 tcatttcaat tacatcgttt tgatttttaa cagttttcac ctcaacaaat caaatggcat
     3421 tagcattcat catggaagaa agactgataa aaatgatcat tttcaatgac ttatgatcac
     3481 accgggtatt gccaagtcat tccttttacg ccaggatacc cacaagtgag tagtcgagga
     3541 gagtttgcca atttttgcga tataaatttc aatgtccgaa gaaccattaa ttgttaattg
     3601 attctgtata aggtattaac atgacaattt catatattcc tattctgaaa gcgaaacgtt
     3661 ctgagttatc agctttatcg cagctatcca ttgaaaagaa atcaaaaatt ttgccattac
     3721 tcgaaattga accagtgcca attgaccctg attcaggtat tgccttaaag agttataacg
     3781 agactcttat agagttcgga aagaaagtct caaaatcttg ctcagatatg caaggtgttt
     3841 atattgatgg attattaatt gaagagcatt ttatt
//
LOCUS       DAZKHF020000321         3853 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2201S31241
            SAMN43267800-rid25106413.denovo.323, whole genome shotgun sequence.
ACCESSION   DAZKHF020000321 DAZKHF020000000
VERSION     DAZKHF020000321.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267800
            Sequence Read Archive: SRR30307263
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3853)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3853)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 3853)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350581.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350581.2
            Genome Coverage       :: 151x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:21:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,652
            CDSs (total)                      :: 5,557
            Genes (coding)                    :: 5,320
            CDSs (with protein)               :: 5,320
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 237
            CDSs (without protein)            :: 237
            Pseudo Genes (ambiguous residues) :: 0 of 237
            Pseudo Genes (frameshifted)       :: 102 of 237
            Pseudo Genes (incomplete)         :: 130 of 237
            Pseudo Genes (internal stop)      :: 52 of 237
            Pseudo Genes (multiple problems)  :: 41 of 237
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3853
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267800-rid25106413.denovo.323"
                     /serotype="O 26:H11"
                     /isolate="2201S31241"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-01"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            complement(202..1851)
                     /gene="pgi"
                     /locus_tag="ACHOSC_RS22200"
                     /old_locus_tag="pgaptmp_004440"
     CDS             complement(202..1851)
                     /gene="pgi"
                     /locus_tag="ACHOSC_RS22200"
                     /old_locus_tag="pgaptmp_004440"
                     /EC_number="5.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004347 - glucose-6-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate isomerase"
                     /protein_id="HGU4550239.1"
                     /translation="MKNINPTQTAAWQALQKHFDEMKDVTIADLFAKDGDRFSKFSAT
                     FGDQMLVDYSKNRITEETLAKLQDLAKECDLAGAIKSMFSGEKINRTENRAVLHVALR
                     NRSNTPILVDGKDVMPEVNAVLEKMKTFSEAIISGEWKGYTGKAITDVVNIGIGGSDL
                     GPYMVTEALRPYKNHLNMHFVSNVDGTHIAEVLKKVNPETTLFLVASKTFTTQETMTN
                     AHSARDWFLKAAGDEKHVAKHFAALSTNAKAVGEFGIDTANMFEFWDWVGGRYSLWSA
                     IGLSIVLSIGFDNFVELLSGAHAMDKHFSTTPAEKNLPVLLALVGIWYNNFFGAETEA
                     ILPYDQYMHRFAAYFQQGNMESNGKYVDRNGNVVDYQTGPIIWGEPGTNGQHAFYQLI
                     HQGTKMVPCDFIAPAITHNPLSDHHQKLLSNFFAQTEALAFGKSREVVEQEYRDQGKD
                     PATLDYVVPFKVFEGNRPTNSILLREITPFSLGALIALYEHKIFTQGVILNIFTFDQW
                     GVELGKQLANRILPELKDGKEISSHDSSTNGLINRYKAWRG"
     regulatory      2088..2284
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00168"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Lysine riboswitch is most abundant in Firmicutes
                     and Gammaproteobacteria where they are found upstream of a
                     number of genes involved in lysine biosynthesis, transport
                     and catabolism; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="lysine"
                     /db_xref="RFAM:RF00168"
     gene            2376..3725
                     /gene="lysC"
                     /locus_tag="ACHOSC_RS22205"
                     /old_locus_tag="pgaptmp_004441"
     CDS             2376..3725
                     /gene="lysC"
                     /locus_tag="ACHOSC_RS22205"
                     /old_locus_tag="pgaptmp_004441"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004072 - aspartate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysine-sensitive aspartokinase 3"
                     /protein_id="HGU4550240.1"
                     /translation="MSEIVVSKFGGTSVADFDAMNRSADIVLSDANVRLVVLSASAGI
                     TNLLVALAEGLEPGERFEKLDAIRNIQFAILERLRYPNVIREEIERLLENITVLAEAA
                     ALATSPALTDELVSHGELMSTLLFVEILRERDVQAQWFDVRKVMRTNDRFGRAEPDIA
                     ALAELAALQLLPRLNEGLVITQGFIGSENKGRTTTLGRGGSDYTAALLAEALHASRVD
                     IWTDVPGIYTTDPRVVSAAKRIDEIAFAEAAEMATFGAKVLHPATLLPAVRSDIPVFV
                     GSSKDPRAGGTLVCNKTENPPLFRALALRRNQTLLTLHSLNMLHSRGFLAEVFGILAR
                     HNISVDLITTSEVSVALTLDTTGSTSTGDTLLTQSLLMELSALCRVEVEEGLALVALI
                     GNDLSKACGVGKEVFGVLEPFNIRMICYGASSHNLCFLVPGEDAEQVVQKLHFNLFE"
BASE COUNT          893 a          986 c         1009 g          965 t
ORIGIN      
        1 atttatctga taaaaaaatg ccgacataac gtcggcattg ttattaaggt tatttaatca
       61 atcgtgctat gtattaattg ccgaatacga tgtacatatc ggcatcgacc tgtaggcctg
      121 ataagacgcg accgcgtcgc atcaggcatc ggttgccgga tgcggcgtga acgccttatc
      181 cggcctacat atcgacgatg attaaccgcg ccacgcttta tagcggttaa tcagaccatt
      241 ggtcgaacta tcgtggctgc tgatttcttt gccatctttc agctctggca gaatacggtt
      301 cgccagctgt ttacccagct ccacgcccca ctggtcgaag gtgaagatgt tcagaatcac
      361 gccctgagta aagattttgt gctcatacag cgcaatcaac gcacccaggc tgaacggagt
      421 gatttcacgc agcaggatgg agttggtcgg gcggttacct tcgaacactt tgaacggcac
      481 cacgtagtca agcgttgccg gatctttacc ctgatcgcga tattcctgct caaccacttc
      541 gcgggattta ccaaacgcca gcgcttcggt ctgggcgaag aagttagaca gcagtttctg
      601 gtgatgatca gagagcgggt tatgggtgat agccggagcg atgaaatcgc acggtaccat
      661 tttggttccc tggtggatca gctggtagaa cgcgtgctga ccgttagtgc ctggttcacc
      721 ccagataatc gggccagtct ggtaatccac aacgttaccg ttacggtcaa catacttacc
      781 gttggactcc atattgccct gctggaagta cgccgcgaaa cggtgcatat actggtcata
      841 cggcagaatc gcctcagttt ccgcaccaaa gaaattgttg taccagatgc caaccagcgc
      901 cagcagtaca ggcaggtttt tctcggcagg cgtggtggag aaatgcttgt ccatcgcgtg
      961 tgcgccagaa agcagttcaa cgaagttatc aaagccgatg gagagaacaa tcgacaggcc
     1021 aatcgctgac cacaaagagt aacggccgcc gacccagtcc cagaactcga acatgttggc
     1081 agtatcaata ccaaactcgc caacggcttt ggcattggtt gagagcgccg caaagtgttt
     1141 cgcaacgtgt ttttcatcac ctgccgcttt caggaaccag tcacgcgcgc tatgggcgtt
     1201 ggtcatggtt tcctgagtgg tgaaggtttt agatgctacc aggaacagcg tggtttccgg
     1261 gtttactttt ttcagcactt cagcgatgtg agtcccatcg acgttagaaa caaagtgcat
     1321 gttcaggtga tttttgtacg gacgcagtgc ttcggtcacc atgtacgggc cgaggtcaga
     1381 accgccgatc ccgatgttca ctacgtcagt gattgctttg ccggtataac ccttccactc
     1441 accggagata atcgcttccg agaaggtttt catcttctcc agcaccgcgt tgacttccgg
     1501 cattacgtct ttgccatcaa ccaaaatcgg ggtattgcta cggttacgca gcgctacgtg
     1561 cagcacagcg cggttttcag tgcggttgat cttctcgcca gagaacatcg acttaatcgc
     1621 gcccgccaga tcgcactctt tcgccagatc ttgtaatttc gccagcgtct cttcagtgat
     1681 gcggtttttg gagtaatcca ccagcatctg atcgccgaag gttgcggaga acttagaaaa
     1741 acgatcgccg tctttagcaa aaagatcggc gatcgtaacg tctttcattt catcgaagtg
     1801 tttctgtagt gcctgccagg cagcggtctg cgttggattg atgtttttca ttagcaatac
     1861 tcttctgatt ttgagaattg tgactttgga agattgtagc gccagtcaca gaaaaatgtg
     1921 atggttttag tgccgttagc gtaatgttga gtgtaaaccc ttagcgcagt gaagcattta
     1981 ttagctgaac tactgaccgc caggagtgga tgaaaaatcc gcatgacccc gtcgttgaca
     2041 accgccccgc tcacccttta tttataaatg tactacctgc gctagcgcag gccagaagag
     2101 gcgcgttgcc caagtaacgg tgttggagga gccagtcctg tgataacacc tgagggggtg
     2161 catcgccgag gtgattgaac ggctggccac gttcatcatc ggctacaggg gctgaatccc
     2221 ctgggttgtc accagaagcg ttcgcagtcg ggcgtttcgc aagtggtgga gcacttctgg
     2281 gtgaaaatag tagcgaagta tcgctctgcg cccacccgtc ttccgctctt cccttgtgcc
     2341 aaggctgaaa atggatcccc tgacacgagg tagttatgtc tgaaattgtt gtctccaaat
     2401 ttggcggtac cagcgtagct gattttgacg ccatgaaccg cagcgctgat attgtgcttt
     2461 ctgatgccaa cgtgcgttta gttgtcctct cggcttctgc tggtatcact aatctgctgg
     2521 tcgctttagc tgaaggactg gaacctggcg agcgattcga aaaactcgac gctatccgca
     2581 acatccagtt tgccattctg gaacgtctgc gttacccgaa cgttatccgt gaagagattg
     2641 aacgtctgct ggagaacatt actgttctgg cagaagcggc ggcgctggca acgtctccgg
     2701 cgctgacaga tgagctggtc agccacggcg agctgatgtc gaccctgctg tttgttgaga
     2761 tcctgcgcga acgcgatgtt caggcacagt ggtttgatgt acgtaaagtg atgcgtacca
     2821 acgaccgatt tggtcgtgca gagccagata tagccgcgct ggcggaactg gccgcgctgc
     2881 agctgctccc acgtctcaat gaaggcttag tgatcaccca gggatttatc ggtagcgaaa
     2941 ataaaggtcg tacaacgacg cttggccgtg gaggcagcga ttatacggca gccttgctgg
     3001 cggaggcttt acacgcatct cgtgttgata tctggaccga cgtcccgggc atctacacca
     3061 ccgatccacg cgtagtttcc gcagcaaaac gcattgatga aatcgcgttt gccgaagcgg
     3121 cagagatggc aacttttggt gcaaaagtac tgcatccggc aacgttgcta cccgcagtac
     3181 gcagcgatat cccggtcttt gtcggctcca gcaaagaccc acgcgcaggt ggtacgctgg
     3241 tgtgcaataa aactgaaaat ccgccgctgt tccgcgcgct ggcgcttcgt cgcaatcaga
     3301 ctctgctcac tttgcacagc ctgaatatgc tgcattctcg cggcttcctc gcggaagttt
     3361 tcggcatcct cgcgcggcat aatatttcgg tagacttaat caccacgtca gaagtgagcg
     3421 tggcattaac ccttgatacc accggttcaa cctccactgg cgatacgttg ctgacgcaat
     3481 ctctgctgat ggagctttcc gcactgtgcc gggtggaagt agaagaaggt ctggcgctgg
     3541 tcgcgttgat tggcaatgac ctgtcaaaag cctgcggcgt tggcaaagag gtattcggcg
     3601 tactggaacc gttcaacatt cgcatgattt gttacggcgc atccagccat aacctgtgct
     3661 tcctggtgcc cggcgaagat gccgagcagg tggtgcagaa gctgcatttt aatttgtttg
     3721 agtaatattt ccctactggc gtataacgct caccgattgt tatacgccat cctcactgaa
     3781 aacatagatt aaataatatc tgaaaagcaa aatcactcct gtagaacagt attataatca
     3841 ccgaaaaata tca
//
LOCUS       DAZKHF020000322         3840 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2201S31241
            SAMN43267800-rid25106413.denovo.324, whole genome shotgun sequence.
ACCESSION   DAZKHF020000322 DAZKHF020000000
VERSION     DAZKHF020000322.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267800
            Sequence Read Archive: SRR30307263
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3840)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3840)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 3840)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350581.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350581.2
            Genome Coverage       :: 151x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:21:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,652
            CDSs (total)                      :: 5,557
            Genes (coding)                    :: 5,320
            CDSs (with protein)               :: 5,320
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 237
            CDSs (without protein)            :: 237
            Pseudo Genes (ambiguous residues) :: 0 of 237
            Pseudo Genes (frameshifted)       :: 102 of 237
            Pseudo Genes (incomplete)         :: 130 of 237
            Pseudo Genes (internal stop)      :: 52 of 237
            Pseudo Genes (multiple problems)  :: 41 of 237
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3840
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267800-rid25106413.denovo.324"
                     /serotype="O 26:H11"
                     /isolate="2201S31241"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-01"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            complement(85..429)
                     /gene="yoaB"
                     /locus_tag="ACHOSC_RS22210"
                     /old_locus_tag="pgaptmp_004442"
     CDS             complement(85..429)
                     /gene="yoaB"
                     /locus_tag="ACHOSC_RS22210"
                     /old_locus_tag="pgaptmp_004442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416323.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HGU4550241.1"
                     /translation="MTIVRIDAEARWSDVVIHNNTLYYTGVPENLDADAFEQTANTLA
                     QIDAVLEKQGSNKSSILDATIFLADKNDFAAMNKAWDAWVVAGHAPVRCTVQAGLMNP
                     KYKVEIKIVAAV"
     gene            561..2471
                     /gene="yoaA"
                     /locus_tag="ACHOSC_RS22215"
                     /old_locus_tag="pgaptmp_004443"
     CDS             561..2471
                     /gene="yoaA"
                     /locus_tag="ACHOSC_RS22215"
                     /old_locus_tag="pgaptmp_004443"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase"
                     /protein_id="HGU4550242.1"
                     /translation="MTDDFAPDGQLAKAIPGFKPREPQRQMAVAVTQAIEKGQPLVVE
                     AGTGTGKTYAYLAPALRAKKKVIISTGSKALQDQLYSRDLPTVSKALKYTGNVALLKG
                     RSNYLCLERLEQQALAGGDLPVQILSDVILLRSWSNQTVDGDISTCVSVAEDSQAWPL
                     VTSTNDNCLGSDCPMYKDCFVVKARKKAMDADVVVVNHHLFLADMVVKESGFGELIPE
                     ADVMIFDEAHQLPDIASQYFGQSLSSRQLLDLAKDITIAYRTELKDTQQLQKCADRLA
                     QSAQDFRLQLGEPGYRGNLRELLANPQIQRAFLLLDDTLELCYDVAKLSLGRSALLDA
                     AFERATLYRTRLKRLKEINQPGYSYWYECTSRHFTLALTPLSVADKFKELMAQKPGSW
                     IFTSATLSVNDDLHHFTSRLGIEQAESLLLPSPFDYSRQALLCVPRNLPQTNQPGSAR
                     QLAAMLRPIIEANNGRCFMLCTSHAMMRDLAEQFRATMTLPVLLQGETSKGQLLQQFV
                     SAGNALLVATSSFWEGVDVRGDTLSLVIIDKLPFTSPDDPLLKARMEDCRLRGGDPFD
                     EVQLPDAVITLKQGVGRLIRDADDRGVLVICDNRLVMRPYGATFLASLPPAPRTRDIA
                     RAVRFLAIPSSR"
     gene            2529..3224
                     /gene="tsaB"
                     /locus_tag="ACHOSC_RS22220"
                     /old_locus_tag="pgaptmp_004444"
     CDS             2529..3224
                     /gene="tsaB"
                     /locus_tag="ACHOSC_RS22220"
                     /old_locus_tag="pgaptmp_004444"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0002949 - tRNA threonylcarbamoyladenosine
                     modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     dimerization subunit type 1 TsaB"
                     /protein_id="HGU4550243.1"
                     /translation="MRILAIDTATEACSVALWNDGTVNAHFELCPREHTQRILPMVQD
                     ILTTSGTSLTDINALVYGRGPGSFTGVRIGIGIAQGLALGAELPMIGVSTLMTMAQGA
                     WRKNGATRVLAAIDARMGEVYWAEYQRDENGIWRGEETEAVLKPELVHERMQQLSGEW
                     VTVGTGWQAWPDLGKESGLVLRDGEVLLPAAEDMLPIACQMFAEGKTVAVEHAEPVYL
                     RNNVAWKKLPGKE"
     gene            3264..>3840
                     /gene="yeaY"
                     /locus_tag="ACHOSC_RS22225"
                     /old_locus_tag="pgaptmp_004445"
     CDS             3264..>3840
                     /gene="yeaY"
                     /locus_tag="ACHOSC_RS22225"
                     /old_locus_tag="pgaptmp_004445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Slp family lipoprotein YeaY"
                     /protein_id="HGU4550244.1"
                     /translation="MAVQKNVIKGILAGTFALMLSGCVTVPDAIKGSSPTPQQDLVRV
                     MSAPQLYVGQEARFGGKVVAVQNQQGKTRLEIATVPLDSGARPTLGEPSRGRIYADVN
                     GFLDPVDFRGQLVTVVGPITGAVDGKIGNTPYKFMVMQVTGYKRWHLTQQVIMPPQPI
                     DPWFYGGRGWPYGYGGWGWYNPGPARVQTVVT"
BASE COUNT          888 a          991 c         1074 g          887 t
ORIGIN      
        1 atatcttaat ctctggtcga tttatgtaaa accgaccagt attatcgata aattatcgtc
       61 tgacttattt tgcttcgata aagcttacac cgcagccaca atcttaattt caactttata
      121 cttcgggttc atcaaacccg cttgtaccgt gcagcgcacc ggcgcatgac ccgcgacaac
      181 ccaggcatcc cacgctttat tcatcgccgc gaagtcgttt ttatcggcca ggaaaatggt
      241 ggcatccaga atgctcgatt tattgctgcc ctgtttttcc agcacggcat caatctgcgc
      301 cagcgtgttg gcggtttgct caaaggcatc ggcgtcgagg ttttccggta caccagtgta
      361 gtagagcgtg ttgttgtgga ttaccacatc agaccagcgg gcttcagcat cgatacgaac
      421 gatagtcatc attttgtcct cattatcctt ccattttcgt gctgcaaaga ctgccacaat
      481 gtgaggtcgc cgtcaccaat tcaactggcg aaagcgccct catcctgaca taatccctgt
      541 tcaaatcaac agggggtagt gtgacggacg attttgcacc agacggtcag ctggcgaaag
      601 cgataccagg ctttaagccg cgagaaccac agcgacagat ggcggtagcc gtcacccagg
      661 cgatagaaaa aggccagccg ctggtggtgg aagcaggaac cggtacgggc aaaacctacg
      721 cttacctagc tcctgcgctg cgggcgaaaa agaaagtcat tatctcgacc ggctcaaaag
      781 cgttgcagga tcagctctac agccgcgatt tgccaacagt ctcaaaggca ttgaaatata
      841 cgggcaacgt ggcgctgctg aaagggcgct caaactacct ctgcctcgag cgtctcgaac
      901 agcaggcgct ggcggggggc gatctgccgg tacaaatctt aagcgatgtg atcctgctgc
      961 gctcctggtc taatcaaaca gtcgatggtg atatcagcac ctgcgtcagc gtggcggaag
     1021 attcacaggc gtggccgctg gtcaccagca ccaacgacaa ctgtcttggc agcgactgcc
     1081 cgatgtataa agattgcttt gtggtcaaag cacgtaaaaa agcgatggac gccgatgtgg
     1141 tggtggtaaa ccatcatctc tttctggcgg atatggtggt taaagagagt ggatttggcg
     1201 aactgatccc ggaagcggac gtcatgatct tcgacgaagc ccaccagcta ccggacattg
     1261 ccagccagta ttttggtcag tcactctcca gtcgacaact gctcgacctg gcaaaagaca
     1321 tcaccatcgc ttaccgcacc gaattaaaag acacccagca gttacaaaag tgcgctgatc
     1381 gtcttgccca gagtgcgcag gattttcgtc tgcaactcgg tgagccaggt tatcgcggta
     1441 acctgcgtga gctgttagct aatccgcaaa ttcagcgggc atttttactg ctcgatgaca
     1501 ccctggaact ttgttatgac gtggcgaaac tgtcactggg gcgttccgcc ttgctggatg
     1561 cggcatttga gcgcgctacg ttgtatcgca cacggctgaa gcggctaaaa gagatcaatc
     1621 agccgggcta cagctactgg tacgaatgca cttcgcgcca ttttactctg gctctcacgc
     1681 cgctcagcgt ggcggataaa ttcaaagagt taatggcgca aaagcctggt agctggatct
     1741 tcacctcagc aacgctgtcg gtgaacgacg atctgcatca tttcacctcg cggcttggga
     1801 tagaacaggc ggagtcgttg ctattgccaa gcccgtttga ttacagccgc caggcgttac
     1861 tctgtgtgcc gcgcaatctg ccgcaaacca accagccagg ttctgctcgc cagttagcgg
     1921 caatgctgcg accgatcatc gaagctaaca acggtcgttg ttttatgctt tgtacctcgc
     1981 acgccatgat gcgtgatctg gccgagcagt tccgcgctac catgacgctt cccgttttgt
     2041 tgcaggggga aaccagcaaa gggcaactgt tgcagcaatt tgtcagcgcc ggtaacgcgc
     2101 ttcttgtggc aaccagtagt ttctgggaag gggtggacgt gcgtggcgat acattgtcat
     2161 tggtgattat cgacaagttg ccgtttacct cgccggatga tccactgtta aaagcgcgca
     2221 tggaagattg tcgtttgcgc ggtggcgacc cgttcgatga agtgcaacta ccagatgccg
     2281 tcattactct caaacagggg gtagggcgac tgattcgcga cgccgacgat cgtggcgtgc
     2341 tggtgatttg tgacaatcgg ctggtgatgc gtccttacgg cgcgacgttt ctcgccagtc
     2401 tgccgcccgc gccacgcact cgtgacattg cccgtgcggt tcgtttcctt gcgataccat
     2461 cctccaggta atttgttacg gagtatggta tgatgcgcgc ctttttattg atctaagcac
     2521 gaagacccat gcgaattctg gctatcgata ccgcgacaga ggcctgctct gtcgccctgt
     2581 ggaacgacgg tactgtcaac gctcattttg agctttgccc tcgtgaacat actcaacgaa
     2641 tcttaccgat ggtgcaggat atcctgacca ccagcggaac ttccctgact gatattaacg
     2701 ctctggttta cgggcgcggc cccggtagct ttaccggcgt gcgcattggt attggcattg
     2761 cgcaagggct ggcacttggc gcagagctac cgatgattgg cgtctccacg ctaatgacga
     2821 tggcgcaagg ggcgtggcgc aaaaacggcg caacccgcgt gctggcagcc attgacgcgc
     2881 gaatgggcga agtttactgg gccgaatatc agcgtgatga aaacggtatc tggcgcggtg
     2941 aagaaaccga agccgtactc aaacccgaac tcgttcatga acgaatgcaa cagctttccg
     3001 gcgaatgggt aacggtaggg acgggctggc aagcctggcc ggatctcggt aaagagagcg
     3061 ggctggtttt gcgcgacgga gaagttttac tgccagcggc ggaagatatg ctgccgattg
     3121 cgtgtcagat gtttgccgag ggtaaaacgg tggcggtgga acatgccgaa ccggtttatt
     3181 tacgtaacaa cgtcgcatgg aagaaacttc cgggcaaaga atgaatctta agagtatgcc
     3241 ctgagaaaaa aaggagtcgc atcatggcgg ttcaaaagaa tgttatcaaa ggcatactgg
     3301 caggtacgtt tgcgctaatg ctgagcggtt gtgtcactgt gccggacgcc attaaaggca
     3361 gcagtcccac gccgcaacaa gatttagttc gggtgatgag tgcgccgcag ctgtacgttg
     3421 gtcaggaggc acgctttggt ggcaaagtgg ttgcggtaca aaaccagcaa gggaaaaccc
     3481 gcctggaaat tgctaccgta ccgctggaca gcggagccag accgacgctg ggagaacctt
     3541 ctcgcggtcg catttatgcc gatgtgaacg gttttctgga cccggtggat ttccgtggac
     3601 aacttgttac ggtagtcggg ccaatcactg gtgcggttga cggcaaaatc ggcaatacgc
     3661 cctataaatt tatggtgatg caagtaacgg gttacaaacg ttggcattta acccagcagg
     3721 tgattatgcc gcctcagccg attgatccat ggttttatgg cggtcgtggc tggccctatg
     3781 gctacggcgg atggggctgg tataatcccg gccccgcgag agtacaaaca gttgtaactg
//
LOCUS       DAZKHF020000323         3805 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2201S31241
            SAMN43267800-rid25106413.denovo.325, whole genome shotgun sequence.
ACCESSION   DAZKHF020000323 DAZKHF020000000
VERSION     DAZKHF020000323.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267800
            Sequence Read Archive: SRR30307263
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3805)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3805)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 3805)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350581.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350581.2
            Genome Coverage       :: 151x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:21:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,652
            CDSs (total)                      :: 5,557
            Genes (coding)                    :: 5,320
            CDSs (with protein)               :: 5,320
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 237
            CDSs (without protein)            :: 237
            Pseudo Genes (ambiguous residues) :: 0 of 237
            Pseudo Genes (frameshifted)       :: 102 of 237
            Pseudo Genes (incomplete)         :: 130 of 237
            Pseudo Genes (internal stop)      :: 52 of 237
            Pseudo Genes (multiple problems)  :: 41 of 237
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3805
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267800-rid25106413.denovo.325"
                     /serotype="O 26:H11"
                     /isolate="2201S31241"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-01"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            226..2142
                     /gene="dnaK"
                     /locus_tag="ACHOSC_RS22230"
                     /old_locus_tag="pgaptmp_004446"
     CDS             226..2142
                     /gene="dnaK"
                     /locus_tag="ACHOSC_RS22230"
                     /old_locus_tag="pgaptmp_004446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001737966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaK"
                     /protein_id="HGU4550245.1"
                     /translation="MGKIIGIDLGTTNSCVAIMDGTTPRVLENAEGDRTTPSIIAYTQ
                     DGETLVGQPAKRQAVTNPQNTLFAIKRLIGRRFQDEEVQRDVSIMPFKIIAADNGDAW
                     VEVKGQKMAPPQISAEVLKKMKKTAEDYLGEPVTEAVITVPAYFNDAQRQATKDAGRI
                     AGLEVKRIINEPTAAALAYGLDKGTGNRTIAVYDLGGGTFDISIIEIDEVDGEKTFEV
                     LATNGDTHLGGEDFDSRLINYLVEEFKKDQGIDLRNDPLAMQRLKEAAEKAKIELSSA
                     QQTDVNLPYITADATGPKHMNIKVTRAKLESLVEDLVNRSIEPLKVALQDAGLSVSDI
                     DDVILVGGQTRMPMVQKKVAEFFGKEPRKDVNPDEAVAIGAAVQGGVLTGDVKDVLLL
                     DVTPLSLGIETMGGVMTTLIAKNTTIPTKHSQVFSTAEDNQSAVTIHVLQGERKRAAD
                     NKSLGQFNLDGINPAPRGMPQIEVTFDIDADGILHVSAKDKNSGKEQKITIKASSGLN
                     EDEIQKMVRDAEANAEADRKFEELVQTRNQGDHLLHSTRKQVEEAGDKLPADDKTAIE
                     SALTALETALKGEDKAAIEAKMQELAQVSQKLMEIAQQQHAQQQTAGADASANNAKDD
                     DVVDAEFEEVKDKK"
     gene            2231..3361
                     /gene="dnaJ"
                     /locus_tag="ACHOSC_RS22235"
                     /old_locus_tag="pgaptmp_004447"
     CDS             2231..3361
                     /gene="dnaJ"
                     /locus_tag="ACHOSC_RS22235"
                     /old_locus_tag="pgaptmp_004447"
                     /EC_number="1.8.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA];
                     GO_process: GO:0009408 - response to heat [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaJ"
                     /protein_id="HGU4550246.1"
                     /translation="MAKQDYYEILGVSKTAEEREIKKAYKRLAMKYHPDRNQGDKEAE
                     AKFKEIKEAYEVLTDSQKRAAYDQYGHAAFEQGGMGGGGFGGGADFSDIFGDVFGDIF
                     GGGRGRQRAARGADLRYNMELTLEEAVRGVTKEIRIPTLEECDVCHGSGAKPGTQPQT
                     CPTCHGSGQVQMRQGFFAVQQTCPHCQGRGTLIKDPCNKCHGHGRVERSKTLSVKIPA
                     GVDTGDRIRLAGEGEAGEHGAPAGDLYVQVQVKQHPIFEREGNNLYCEVPINFAMAAL
                     GGEIEVPTLDGRVKLKVPGETQTGKLFRMRGKGVKSVRGGAQGDLLCRVVVETPVGLN
                     EKQKQLLQELQESFGGPTGEHNSPRSKSFFDGVKKFFDDLTR"
     gene            complement(3465..3617)
                     /gene="mokC"
                     /locus_tag="ACHOSC_RS22240"
                     /old_locus_tag="pgaptmp_004448"
     CDS             complement(3465..3617)
                     /gene="mokC"
                     /locus_tag="ACHOSC_RS22240"
                     /old_locus_tag="pgaptmp_004448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_025292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin MokC"
                     /protein_id="HGU4550247.1"
                     /translation="MKQHKAMIVALIVICITAVVAALVTRKDLCEVHIRTGQTEVAVF
                     TAYESE"
BASE COUNT         1002 a          948 c         1027 g          828 t
ORIGIN      
        1 ctgtcagttt cacgtcttgt cctgctatat cgcgaaattt ctgcgcaaaa gcacaaaaaa
       61 tttttgcatc tcccccttga tgacgtgggt tacgacccca tttagtagtc aaccgcagtg
      121 agtgagtctg caaaaaaatg aaattgggca gttgaaacca gacgtttcgc ccctattaca
      181 gactcacaac cacatgatga ccgaatatat agtggagacg tttagatggg taaaataatt
      241 ggtatcgacc tgggtactac caactcttgt gtagcgatta tggatggcac cactcctcgc
      301 gtgctggaga acgccgaagg cgatcgcacc acgccttcta ttattgccta tacccaggat
      361 ggtgaaactc tggttggtca gccggctaaa cgtcaggcag tgacgaaccc gcaaaacacc
      421 ctgtttgcga ttaaacgcct gattggccgc cgcttccagg acgaagaagt acagcgtgat
      481 gtttccatca tgccgttcaa aattattgct gctgataacg gcgacgcatg ggtcgaagtt
      541 aaaggccaga aaatggcacc gccgcagatt tctgctgaag tgctgaaaaa aatgaagaaa
      601 accgctgaag attacttggg tgaaccggta actgaagctg ttatcaccgt accggcatac
      661 tttaacgatg ctcagcgtca ggcaaccaaa gacgcaggcc gtatcgctgg tctggaagta
      721 aaacgtatca tcaacgaacc gaccgcagct gcgctggctt acggtctgga caaaggtact
      781 ggcaaccgta ctatcgcggt ttatgacctg gggggtggta ctttcgatat ttctattatc
      841 gaaatcgacg aagttgacgg cgaaaaaacc ttcgaagttc tggctaccaa cggtgatacc
      901 cacctgggtg gtgaagactt cgacagccgt ctgatcaact acctggttga agaattcaag
      961 aaagatcagg gcattgacct gcgcaacgat ccgctggcaa tgcagcgcct gaaagaagcg
     1021 gcagaaaaag cgaaaatcga actgtcttcc gctcagcaga ccgacgttaa cctgccgtac
     1081 atcactgcag atgcgaccgg tccgaaacac atgaacatca aagtgactcg tgcgaaactg
     1141 gaaagcctgg ttgaagatct ggtaaaccgt tccattgagc cgctgaaagt tgcactgcag
     1201 gacgctggcc tgtccgtatc tgatatcgac gacgttatcc tcgttggtgg tcagactcgt
     1261 atgccaatgg ttcagaagaa agttgctgag ttctttggta aagagccgcg taaagacgtt
     1321 aacccggacg aagctgtagc aatcggtgct gctgttcagg gtggtgttct gactggtgac
     1381 gtgaaagacg tactgctgct ggacgttacc ccgctgtctc tgggtatcga aaccatgggc
     1441 ggtgtgatga cgacgctgat cgcgaaaaac accactatcc cgaccaagca cagccaggtg
     1501 ttctctaccg ctgaagacaa ccagtctgcg gtaaccatcc atgtgctgca gggtgaacgt
     1561 aaacgtgcgg ctgataacaa atctctgggt cagttcaacc tggatggtat caacccggca
     1621 ccgcgcggca tgccgcagat cgaagttacc ttcgatatcg atgctgacgg tatcctgcac
     1681 gtttccgcga aagataaaaa cagcggtaaa gagcagaaga tcaccatcaa ggcttcttct
     1741 ggtctgaacg aagatgaaat ccagaaaatg gtacgcgacg cagaagctaa cgccgaagct
     1801 gaccgtaagt ttgaagagct ggtacagact cgcaaccagg gcgaccatct gctgcacagc
     1861 acccgtaagc aggttgaaga agcaggcgac aaactgccgg ctgacgacaa aactgctatc
     1921 gagtctgcac tgactgcact ggaaactgct ctgaaaggtg aagacaaagc cgctatcgaa
     1981 gcgaaaatgc aggagctggc acaggtttcc cagaaactga tggaaatcgc ccagcagcaa
     2041 catgcccagc agcagactgc cggtgctgat gcttctgcaa acaacgcgaa agatgacgat
     2101 gttgtcgacg ctgaatttga agaagtcaaa gacaaaaaat aatcgcccta taaacgggta
     2161 attatactga cacgggcgaa ggggaatttc ctctccgccc gtgcattcat ctaggggcaa
     2221 tttaaaaaag atggctaagc aagattatta cgagatttta ggcgtttcca aaacagcgga
     2281 agagcgtgaa atcaaaaagg cctacaaacg cctggccatg aaataccacc cggaccgtaa
     2341 ccagggtgac aaagaggccg aggcgaaatt taaagagatc aaggaagctt atgaagttct
     2401 gaccgactcg caaaaacgtg cggcatacga tcagtatggt catgctgcgt ttgagcaagg
     2461 tggtatgggc ggcggcggtt ttggcggcgg cgcagacttc agcgatattt ttggtgacgt
     2521 tttcggcgat atttttggcg gcggacgtgg tcgtcaacgt gcggcgcgcg gtgctgattt
     2581 acgctataac atggagctca ccctcgaaga agctgtacgt ggcgtgacca aagagatccg
     2641 cattccgact ctggaagagt gtgacgtttg ccacggtagc ggtgcaaaac caggtacaca
     2701 gccgcagacc tgtccgacct gtcatggttc tggtcaggtg cagatgcgtc agggattctt
     2761 cgctgtacag cagacctgtc cacactgtca gggccgcggt acgctgatca aagatccgtg
     2821 caacaaatgt catggtcatg gtcgtgttga gcgcagcaaa acgctgtccg ttaaaatccc
     2881 ggcaggggtg gacactggag accgcatccg tcttgcgggc gaaggtgaag cgggcgagca
     2941 tggcgcaccg gcaggcgatc tgtacgttca ggttcaggtt aaacagcacc cgattttcga
     3001 gcgtgaaggc aacaacctct attgcgaagt cccgatcaac ttcgctatgg cggcgctggg
     3061 tggcgaaatc gaagtaccga cccttgatgg tcgcgtcaaa ctgaaagtgc ctggcgaaac
     3121 ccagaccggt aagcttttcc gtatgcgcgg taaaggcgtc aagtctgtcc gcggtggcgc
     3181 acagggtgat ttgctgtgcc gcgttgtcgt cgaaacaccg gtaggcctga acgagaagca
     3241 gaaacagctg ctgcaagagc tgcaagaaag cttcggtggc ccaaccggtg agcacaacag
     3301 cccgcgctca aagagcttct ttgatggtgt gaagaagttt tttgacgacc tgacccgcta
     3361 acctccccaa aagcctgccc gtgggcaggc ttgggtaaaa ttagggtgcg ttgaagatat
     3421 gcgagcacct gaaaagtggc ggggatcact ccccgccgtt gcacttactc ggattcgtaa
     3481 gccgtgaaaa cagcaacctc cgtctggcca gttcggatgt gaacctcaca gaggtctttt
     3541 ctcgttacca gcgccgccac tacggcggtg atacagatga cgatcagggc gacaatcatc
     3601 gccttatgct gcttcattgc tctcttctcc ttgaccttac ggtcagtaag aggcactcta
     3661 catgtgttca gcatataggg ggcctcgggt tgatagtaaa atatcactcg gggcttttct
     3721 ctatctgccg ttcagctaat gcctgagaca gatagcctca agtacccgcc gctattatat
     3781 cgctctcttt aacccattct gtttt
//
LOCUS       DAZKHF020000324         3801 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2201S31241
            SAMN43267800-rid25106413.denovo.326, whole genome shotgun sequence.
ACCESSION   DAZKHF020000324 DAZKHF020000000
VERSION     DAZKHF020000324.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267800
            Sequence Read Archive: SRR30307263
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3801)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3801)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 3801)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350581.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350581.2
            Genome Coverage       :: 151x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:21:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,652
            CDSs (total)                      :: 5,557
            Genes (coding)                    :: 5,320
            CDSs (with protein)               :: 5,320
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 237
            CDSs (without protein)            :: 237
            Pseudo Genes (ambiguous residues) :: 0 of 237
            Pseudo Genes (frameshifted)       :: 102 of 237
            Pseudo Genes (incomplete)         :: 130 of 237
            Pseudo Genes (internal stop)      :: 52 of 237
            Pseudo Genes (multiple problems)  :: 41 of 237
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3801
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267800-rid25106413.denovo.326"
                     /serotype="O 26:H11"
                     /isolate="2201S31241"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-01"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            <1..641
                     /locus_tag="ACHOSC_RS22245"
                     /old_locus_tag="pgaptmp_004449"
     CDS             <1..641
                     /locus_tag="ACHOSC_RS22245"
                     /old_locus_tag="pgaptmp_004449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF1266 domain-containing protein"
                     /protein_id="HGU4550248.1"
                     /translation="KPAHDSPKMYTGIKEFDLSSSWGINNRDDLIQTIYQMTDDGHAN
                     DLAGLYLTWHRSSPEEWKALIAGGSERGLIYTQFVAQTAMCCGEGGIKAWDYVRMGFL
                     SRVGVLNNWLTEEESLWLQSCVYVRAHHYYHSWMHYFSAYSLGRLYWQSSQCEDNTSL
                     REALTLYKYDSAGSRMFEELAAGSDRFYATLPWQPLTVQPECPVTLKDVSDL"
     gene            638..2089
                     /gene="djlC"
                     /locus_tag="ACHOSC_RS22250"
                     /old_locus_tag="pgaptmp_004450"
     CDS             638..2089
                     /gene="djlC"
                     /locus_tag="ACHOSC_RS22250"
                     /old_locus_tag="pgaptmp_004450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone DjlC"
                     /protein_id="HGU4550249.1"
                     /translation="MKTCWQILEIESTTQIDIIRQAYLARLPLCHPETDPQGFKALRQ
                     AYEEALRLAVNPVEEADDEEKDAAAEHEILRAFRTLLDSESDRFQPSAWQKFIQQLNT
                     WNMEDVDQLRWPLCAIAIEARYLSLNCASLLAERLNWHSFNDSEGMDEEEREAFLEAI
                     QAGDCFDFLSLLEYPVALQNQTVEYYFALERCCRYHPDYVTAFLAMEGPWFIPDDAKL
                     HRKLLRWYSSVQTGMAELIPVAKQWQMEEPESEDARYYLCAQRLYCGEGESLLADLCA
                     YRESYPSTQADNLLLQWSKRHCPDYFALLVMVIEARSMVDAQGQPLKYVPGESARTRL
                     LWAEILHSGKLSPLGQSFIESLFFKRKAWAWWKSRVGSETEEDSPLLDLYRVAEQVVL
                     EAFPKQEMLARLNTRLEGGDAHPLEAIITRMLLTKVKLEPEDEDVDEPTPENHEEKND
                     EGEKPQSITSIIKISLTVLVIGYALGKIAMLFS"
     gene            complement(2149..>3801)
                     /gene="hscC"
                     /locus_tag="ACHOSC_RS22255"
                     /old_locus_tag="pgaptmp_004451"
     CDS             complement(2149..>3801)
                     /gene="hscC"
                     /locus_tag="ACHOSC_RS22255"
                     /old_locus_tag="pgaptmp_004451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000367910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone HscC"
                     /protein_id="HGU4550250.1"
                     /translation="AIGIDLGTTNSLIAVWKDGAAQLIPNKFGEYLTPSIISMDENNH
                     ILVGKPAVSRRTSHPDKTAALFKRAMGSNTNWRLGSDTFNAPELSSLVLRSLKEDAEE
                     FLQRPIKDVVISVPAYFSDEQRKHTRLAAELAGLNAVRLINEPTAAAMAYGLHTQQNT
                     RSLVFDLGGGTFDVTVLEYATPVIEVHASAGDNFLGGEDFTHMLVDEVLKRADVARTT
                     LNESELGALYACVEAAKCSNQSPLHIRWQYQDETRECEFYENELEDLWLPLLNRLRVP
                     IEQALRDARLKPSQIDSLVLVGGASQMPLVQRIAVRLFGKLPYQSYDPSTIVALGAAI
                     QAACRLRSEDIEEVILTDICPYSLGVEVNRQGVSGIFSPIIERNTTVPVSRVETYSTM
                     HPEQNSITVNVYQGENHKVKNNILVESFDVPLKKTGAYQSIDIRFSYDINGLLEVDVL
                     LEDGSVKSRVINHSPVTLSAQQIEESRTRLSALKIYPRDMLINRTFKAKLEELWARAL
                     GDEREEIGRVITDFDAALQSNDMARVDEVRRRASVYLAIETS"
BASE COUNT          968 a          906 c          960 g          967 t
ORIGIN      
        1 gcaaacccgc gcatgactcg ccaaaaatgt ataccgggat aaaagaattc gatctctcat
       61 catcctgggg aataaataat cgtgatgatt taattcagac tatttatcag atgactgatg
      121 atggtcatgc caatgattta gctggcttat atctgacatg gcatcgctct tcccctgaag
      181 agtggaaagc attaattgct ggtggctcgg agagagggtt gatttatacc caattcgttg
      241 cccaaaccgc tatgtgctgt ggagaagggg gaatcaaagc ctgggattat gttcgaatgg
      301 ggtttctgtc acgggtcggt gtgctcaaca actggctgac ggaagaagag agtttatggc
      361 tgcaatcgtg cgtctatgta cgagctcacc attactatca cagctggatg cactattttt
      421 ccgcctattc gttaggacgt ctctactggc aatcttctca gtgcgaagac aacacttcgc
      481 tacgcgaggc gctaaccctg tacaaatacg atagcgccgg gagtcggatg ttcgaagagc
      541 tggcagcggg aagcgatcgt ttctatgcca cgcttccctg gcagccatta actgtccagc
      601 ctgaatgccc ggtaacgctt aaggatgtga gcgacctatg aaaacatgtt ggcaaatcct
      661 cgaaattgaa agcacgacgc aaatagacat tatccgccag gcttatcttg ctcgcttacc
      721 gttatgtcat cccgaaaccg atccgcaggg gtttaaagca ttacgccagg cctatgaaga
      781 ggcgctgcga ctggcggtaa atcctgtcga ggaagcagat gatgaagaaa aagatgccgc
      841 cgctgaacat gaaatactgc gtgcattcag gacattactg gattcagaaa gtgatcgttt
      901 tcagccctcc gcctggcaga aatttattca gcaattaaat acctggaaca tggaggatgt
      961 cgatcaatta cgctggccgc tgtgtgcaat cgctatagaa gcgcgatatc tttcattaaa
     1021 ttgtgcttct ttgctggcag agcgtttgaa ctggcattcg tttaatgaca gcgaaggaat
     1081 ggatgaggaa gaaagggagg cttttcttga ggccattcag gctggtgatt gtttcgattt
     1141 ccttagcctt ctggaatatc ccgttgcgtt gcagaaccag actgttgagt attacttcgc
     1201 gctggaacgt tgctgccgtt accatcctga ctacgtcact gcgtttttgg cgatggaagg
     1261 tccgtggttc attcctgatg atgcaaagtt acatcgcaaa ctgttgcgct ggtacagctc
     1321 ggtgcaaaca ggtatggcgg aactcattcc tgtagccaaa cagtggcaaa tggaagaacc
     1381 agaaagcgaa gatgcccgat attacttgtg tgctcaaagg ttgtactgcg gcgaggggga
     1441 aagcctgctt gccgatctct gcgcgtaccg ggaaagttac ccatctacac aagctgataa
     1501 tttgttgttg cagtggagca agcgtcattg cccggattat ttcgcgttat tagtgatggt
     1561 tatcgaagcg cggagcatgg tagatgcgca aggtcaaccg ctgaaatatg ttcctggtga
     1621 gagcgcccgg acgcggctgt tatgggcgga aattttacat agcggaaaat tatcgccgtt
     1681 aggtcaatcg tttattgagt cgttattctt caaacgcaaa gcatgggcgt ggtggaaatc
     1741 gagagtcggt agcgagacag aagaagattc accgttactg gatttgtatc gggtagcgga
     1801 acaggtagta cttgaagcgt ttccgaaaca agagatgctg gcccgtctta atacaaggct
     1861 ggaaggcgga gatgctcatc cattagaggc cattatcacc cggatgctgt tgacgaaagt
     1921 aaaactcgag ccggaggatg aagatgtcga tgagccaaca cctgaaaacc atgaagaaaa
     1981 aaatgatgag ggtgaaaaac cacagagcat taccagcatt atcaaaatca gtttaacggt
     2041 gctggttata ggttatgctc tcggcaaaat cgcgatgttg tttagttgac gatgtgtttt
     2101 ttgcgccgat gccggatgca gcatttacgc cgcatccggc agccgtgatt atgaggtctc
     2161 aatggctaaa taaacgctcg cccgccgcct gacctcatcg acgcgggcca tatcgtttga
     2221 ctgcaacgcc gcatcaaaat cggtgatcac ccggccaatc tcttctcgct cgtcacccag
     2281 cgcccgcgcc cacaactctt ccagtttggc cttaaaggtg cggttgatga gcatatcgcg
     2341 cggataaatt ttcaatgcgg ataaccgcgt ccgactctct tcaatctgct gcgcgctcaa
     2401 tgttaccggg ctatgattaa tgactctgga cttaacgctg ccatcttcca gaagcacgtc
     2461 aacttcaagc aagccgttga tatcataact aaagcgaata tcaatcgact gataagcccc
     2521 ggttttcttc aacggcacat cgaaggattc aaccagaatg ttatttttaa ctttgtggtt
     2581 ttctccctga tagacgttaa ccgtaattga attctgttcc gggtgcatgg tcgagtaagt
     2641 ttctacacgc gacacgggta ctgtggtgtt tcgttcaata attggcgaga aaatgccgga
     2701 aacgccctgg cggttaactt caacgcccaa cgagtaaggg caaatatcag tgaggatcac
     2761 ctcttcaata tcttcactgc gtaagcggca ggcggcctgg attgctgcgc ccagcgcgac
     2821 aatggtgctc gggtcgtaac tttgatacgg taatttgcca aacagacgca cggcgattcg
     2881 ctgcaccagc ggcatttgtg acgcgccgcc aaccagcacc agactgtcga tttgactcgg
     2941 cttcagacgt gcatcgcgca acgcctgttc aatcggcact cgcaagcgat tgagcagcgg
     3001 caaccacaaa tcttccagtt cgttctcgta aaattcgcac tcccgcgttt catcctggta
     3061 ctgccagcga atgtgcaatg gcgattgatt gctacatttt gccgcttcca cacaggcgta
     3121 caaggctccc agttcactct cgttaagcgt ggtcctggcg acatccgcgc gttttaaaac
     3181 ctcatcgacc agcatatggg taaaatcttc gccaccaaga aagttgtcgc cagcggaggc
     3241 gtgaacttca atcaccggcg tggcgtactc aagcaccgta acgtcaaacg tgccgccacc
     3301 gagatcaaac accagcgaac gggtattttg ttgggtatgc aggccatacg ccatcgcagc
     3361 tgctgtgggt tcattaatta agcgtaccgc atttaacccg gctaactccg ctgctaaacg
     3421 ggtatgcttg cgttgttcat cgctgaaata agccggaacg gagatcacca catctttaat
     3481 cggacgttgc agaaattctt cggcatcttc ttttaatgag cgtaatacca aagaggacag
     3541 ttctggcgcg ttaaatgtgt cgctgcctaa ccgccagttg gtattactgc ccattgcacg
     3601 tttaaataac gctgccgttt tatccggatg cgaagtacgc cgtgatacag ccggttttcc
     3661 gactaaaata tgattatttt catccatgct aattatggat ggtgttaaat attcaccgaa
     3721 cttatttgga attaattgcg cggcaccgtc tttccagacg gcaattaaac tattggtagt
     3781 gccgagatcg ataccaatgg c
//
LOCUS       DAZKHF020000325         3753 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2201S31241
            SAMN43267800-rid25106413.denovo.327, whole genome shotgun sequence.
ACCESSION   DAZKHF020000325 DAZKHF020000000
VERSION     DAZKHF020000325.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267800
            Sequence Read Archive: SRR30307263
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3753)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3753)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 3753)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350581.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350581.2
            Genome Coverage       :: 151x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:21:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,652
            CDSs (total)                      :: 5,557
            Genes (coding)                    :: 5,320
            CDSs (with protein)               :: 5,320
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 237
            CDSs (without protein)            :: 237
            Pseudo Genes (ambiguous residues) :: 0 of 237
            Pseudo Genes (frameshifted)       :: 102 of 237
            Pseudo Genes (incomplete)         :: 130 of 237
            Pseudo Genes (internal stop)      :: 52 of 237
            Pseudo Genes (multiple problems)  :: 41 of 237
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3753
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267800-rid25106413.denovo.327"
                     /serotype="O 26:H11"
                     /isolate="2201S31241"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-01"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            <1..254
                     /gene="ypdI"
                     /locus_tag="ACHOSC_RS22260"
                     /old_locus_tag="pgaptmp_004452"
     CDS             <1..254
                     /gene="ypdI"
                     /locus_tag="ACHOSC_RS22260"
                     /old_locus_tag="pgaptmp_004452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="colanic acid biosynthesis lipoprotein YpdI"
                     /protein_id="HGU4550251.1"
                     /translation="SLFLLVSIMACNVFAFSISGGVSERSYKETEKTSAMTTTHSTKL
                     QPSQAILFKMREDAPPLNLTEEITPTYPTKANYLIHPVR"
     gene            complement(331..573)
                     /gene="yfdY"
                     /locus_tag="ACHOSC_RS22265"
                     /old_locus_tag="pgaptmp_004453"
     CDS             complement(331..573)
                     /gene="yfdY"
                     /locus_tag="ACHOSC_RS22265"
                     /old_locus_tag="pgaptmp_004453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfdY family protein"
                     /protein_id="HGU4550252.1"
                     /translation="MIYLWMFLALCIVCVSGYIGQVLNVVSAVSSFFGMVILAALIYY
                     FTMWLTGGNELVTGIFMFLAPACGLMIRFMVGYGRR"
     gene            926..1846
                     /gene="lpxP"
                     /locus_tag="ACHOSC_RS22270"
                     /old_locus_tag="pgaptmp_004454"
     CDS             926..1846
                     /gene="lpxP"
                     /locus_tag="ACHOSC_RS22270"
                     /old_locus_tag="pgaptmp_004454"
                     /EC_number="2.3.1.242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311285.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008951 - palmitoleoyl
                     [acyl-carrier-protein]-dependent acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009409 - response to cold [Evidence IEA];
                     GO_process: GO:0036104 - Kdo2-lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="kdo(2)-lipid IV(A) palmitoleoyltransferase"
                     /protein_id="HGU4550253.1"
                     /translation="MFPQCKFSREFLHPRYWLTWFGLGVLWLWVQLPYPVLCFLGTRI
                     GAMARPFLKRRESIARKNLELCFPQHSAEEREKMIAENFRSLGMALVETGMAWFWPDS
                     RVRKWFDVEGLDNLKRAQMQNRGVMVVGVHFMSLELGGRVMGLCQPMMATYRPHNNQL
                     MEWVQTRGRMRSNKAMIGRNNLRGIVGALKKGEAVWFAPDQDYGRKGSSFAPFFAVEN
                     VATTNGTYVLSRLSGAAMLTVTMVRKADYSGYRLFITPEMEGYPTDENQAAAYMNKII
                     EKEIMRAPEQYLWIHRRFKTRPVGESSLYI"
     gene            2202..2273
                     /gene="ypdK"
                     /locus_tag="ACHOSC_RS22275"
                     /old_locus_tag="pgaptmp_004455"
     CDS             2202..2273
                     /gene="ypdK"
                     /locus_tag="ACHOSC_RS22275"
                     /old_locus_tag="pgaptmp_004455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_077777854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein YpdK"
                     /protein_id="HGU4550254.1"
                     /translation="MKYFFMGISFMVIVWAGTFALMI"
     gene            complement(2338..3576)
                     /gene="alaC"
                     /locus_tag="ACHOSC_RS22280"
                     /old_locus_tag="pgaptmp_004456"
     CDS             complement(2338..3576)
                     /gene="alaC"
                     /locus_tag="ACHOSC_RS22280"
                     /old_locus_tag="pgaptmp_004456"
                     /EC_number="2.6.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine transaminase"
                     /protein_id="HGU4550255.1"
                     /translation="MADTRPERRFTRIDRLPPYVFNITAELKMAARRRGEDIIDFSMG
                     NPDGATPPHIVEKLCTVAQRPDTHGYSTSRGIPRLRRAISRWYQDRYDVEIDPESEAI
                     VTIGSKEGLAHLMLATLDHGDTVLVPNPSYPIHIYGAVIAGAQVRSVPLVEGVDFFNE
                     LERAIRESYPKPKMMILGFPSNPTAQCVELEFFEKVVALAKRYDVLVVHDLAYADIVY
                     DGWKAPSIMQVPGARDVAVEFFTLSKSYNMAGWRIGFMVGNKTLVSALARIKSYHDYG
                     TFTPLQVAAIAALEGDQQCVRDIAEQYKRRRDVLVKGLHEAGWMVEMPKASMYVWAKI
                     PEPYAAMGSLEFAKKLLNEAKVCVSPGIGFGDYGDTHVRFALIENRDRIRQAIRGIKA
                     MFRADGLLPASSKHIHENAE"
BASE COUNT          974 a          954 c          880 g          945 t
ORIGIN      
        1 tcagcttatt tttattggtc tctattatgg catgcaatgt ttttgcattt tccatttctg
       61 gtggtgtaag tgagaggagc tataaagaga ctgaaaaaac atcagcgatg acaaccacac
      121 actctacaaa acttcagcca tcacaggcga ttttgtttaa gatgagagaa gatgcgccac
      181 cattaaacct cacagaagaa atcacgccta cttatccgac aaaggcgaat tatcttattc
      241 atcctgtgcg atagcgtctg tcacatggca ggtgaacaat catgtctgat gctgatgtga
      301 acacctgccc aaatgagcca tcctgaccca ctatcgcctg ccatacccca ccataaagcg
      361 aatcatcaag ccacaagccg gggcaagaaa cataaatatc cccgtcacta gttcattacc
      421 gccagttaac cacatggtga agtaataaat gagtgcagcg aggatcacca tgccgaagaa
      481 agaagagacc gccgatacca cgttcagtac ctgaccgatg tagccgctta cgcagacaat
      541 acacagggcg agaaacatcc ataaataaat catagctcaa cctcctgccg cgctaaaaaa
      601 cctgtaactt cagtcacttt aatagtaaga caatccttaa cctctggcaa tttttgctaa
      661 gcatcaatga aaacagcatg ttaaatgcaa gactgttgtg tacggaaaaa tatttacttt
      721 gcacgattaa taatcagtag ctgaaagcag tcagcgagtg ataaagtaca gataccgcat
      781 tcatgctcct cgtggttatg tcctgactag tctttacact ctttacagga accattgtcg
      841 tacatgatgg cccaaccaat tgagtgtacc gctgtcgaca tggaatcttc tgcgatacaa
      901 caattcgtat ctacagaagg taactatgtt tccacaatgc aaattttccc gcgagtttct
      961 acatcctcgc tactggctca catggtttgg gcttggtgta ctctggcttt gggtacagct
     1021 tccttatcct gttctctgct ttctcggcac gcgtattggc gcaatggcgc gaccattcct
     1081 gaaacgtcgt gaatctatcg cccgtaaaaa cctggaactt tgtttcccgc agcattctgc
     1141 ggaagaacgc gagaagatga ttgccgaaaa ctttcgttca ctcggcatgg cgctggtaga
     1201 aaccggcatg gcatggttct ggcccgacag tcgcgtacgt aaatggtttg atgttgaagg
     1261 gttggataac cttaaacgcg cacaaatgca aaatcgcggc gtaatggttg tcggcgtcca
     1321 ttttatgtcg ctggaactgg gcggccgcgt gatgggactg tgccaaccaa tgatggctac
     1381 ctatcgtcca cataataatc agctgatgga atgggtgcag acccgtgggc gcatgcgctc
     1441 taacaaagcg atgatcggca gaaataatct gcgcggcatt gtcggtgcac tgaagaaagg
     1501 tgaagcggta tggtttgctc ccgatcagga ttatggtcgt aaaggcagct ccttcgcgcc
     1561 gttctttgcg gtggaaaatg tcgccacaac caatggcacc tatgttctct cccgtctctc
     1621 tggcgcagcc atgttgaccg taacgatggt aagaaaagcg gattacagcg gatatcgttt
     1681 gttcatcacc ccagagatgg aaggctaccc gacagatgaa aatcaagccg ctgcctatat
     1741 gaacaagatt atcgagaaag agatcatgcg cgcaccggag cagtacctct ggatccaccg
     1801 tcgctttaaa acgcgcccgg tgggagaatc gtcgttgtac atttaacaaa ctgctcttac
     1861 tctttccctc ctcaaatcgg ccataataac ctcatggttt ttatggcttt tttcttaagg
     1921 ttttttcctt cgctgaatat ctaattatta ccgtcagaaa ttaaactgtc gccgatatga
     1981 gacacttgta agttactaaa agtaaataag aaaatgtctc ttgtctcgtt tcagttttag
     2041 gcgtacatta gcgccgtctc gagcagttta gctcagaatt ctggggtgat gcccacggta
     2101 aaatacaggg cttttaaggt cgctttgccc ggtgtcaact cactgtatca ggtgtaatga
     2161 agtcattcag gcgtaacagt aattacgcgg agagatgtaa agtgaaatat ttctttatgg
     2221 gcatttcttt tatggtcatc gtttgggccg gtacttttgc cctgatgatc taaaagcaaa
     2281 ctgtgcaatg aaaaacagga gccatcaggc tcctgtttgc atttctgacg acggtgttta
     2341 ttccgcgttt tcgtgaatat gtttgctgct ggcgggtagt aaaccgtcgg cacggaacat
     2401 cgctttaatc ccacgaatcg cctgacgaat acggtcgcgg ttttcaatca gtgcaaagcg
     2461 aacatgcgta tcaccatagt cgccaaagcc aatccccggc gagacacaaa ccttcgcttc
     2521 gttaaggagc ttcttggcaa attccagcga tcccatggcc gcatatggtt ccgggatttt
     2581 cgcccagaca tacatcgaag ccttcggcat ttcaaccatc cagcccgctt catgcagccc
     2641 tttaaccaat acatcacggc ggcgtttgta ctgttcggca atgtcgcgca cgcactgttg
     2701 atcgccctcc agcgccgcaa tcgccgccac ctgcaacggc gtaaaggtgc cgtaatcgtg
     2761 atagctttta atacgagcca gggcgctgac cagcgttttg ttgccaacca taaagccgat
     2821 acgccagccc gccatgttgt agcttttcga cagcgtaaag aactcgactg ccacatcgcg
     2881 tgcacccggt acctgcatga ttgacggcgc tttccagcca tcgtagacga tatcggcata
     2941 ggccaggtcg tggaccacca gcacatcgta gcgtttcgcc agcgccacaa ccttttcgaa
     3001 gaactccagc tccacgcatt gcgcggttgg attcgacggg aagccgagga tcatcatctt
     3061 cggtttcgga taactttcac gaatggcgcg ctccagttcg ttgaagaaat cgacaccttc
     3121 caccagcggc actgagcgta cctgcgcccc ggcaatcacc gcgccataaa tatggatcgg
     3181 gtaacttgga ttcggcacca gcaccgtgtc accatgatcc agcgtcgcca gcatcagatg
     3241 cgccaggccc tctttcgaac caatagtgac gatggcttct gattccgggt cgatttcaac
     3301 gtcgtagcga tcctgatacc agcgggaaat ggcgcgacgt aaccgcggaa tgccgcgtga
     3361 agtggagtaa ccatgcgtgt ccgggcgctg ggccacagta cataattttt cgacgatatg
     3421 cggcggagtc gcaccgtccg ggttacccat gctgaaatcg ataatatctt cgccgcgccg
     3481 acgcgcagcc attttcagtt cagcggtgat gttaaaaacg tagggcggga gacgatcaat
     3541 gcgcgtaaag cgacgttcag ggcgagtgtc agccataatt tcctcagatt aacgttagcg
     3601 cccggaccgt ccgagcgacg ctgccacaat ggtggctgtt ttgaaaatag cctgattaat
     3661 ttctgtctgt ccagcgtttt agaacatttt tatcaatggc tttaaatgag gaactccact
     3721 tcaaaaacag gaaatttttc tacgctaaca aga
//
LOCUS       DAZKHF020000326         3747 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2201S31241
            SAMN43267800-rid25106413.denovo.328, whole genome shotgun sequence.
ACCESSION   DAZKHF020000326 DAZKHF020000000
VERSION     DAZKHF020000326.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267800
            Sequence Read Archive: SRR30307263
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3747)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3747)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 3747)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350581.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350581.2
            Genome Coverage       :: 151x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:21:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,652
            CDSs (total)                      :: 5,557
            Genes (coding)                    :: 5,320
            CDSs (with protein)               :: 5,320
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 237
            CDSs (without protein)            :: 237
            Pseudo Genes (ambiguous residues) :: 0 of 237
            Pseudo Genes (frameshifted)       :: 102 of 237
            Pseudo Genes (incomplete)         :: 130 of 237
            Pseudo Genes (internal stop)      :: 52 of 237
            Pseudo Genes (multiple problems)  :: 41 of 237
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3747
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267800-rid25106413.denovo.328"
                     /serotype="O 26:H11"
                     /isolate="2201S31241"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-01"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            complement(<1..220)
                     /locus_tag="ACHOSC_RS22285"
                     /old_locus_tag="pgaptmp_004457"
     CDS             complement(<1..220)
                     /locus_tag="ACHOSC_RS22285"
                     /old_locus_tag="pgaptmp_004457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replication protein"
                     /protein_id="HGU4550256.1"
                     /translation="MTNTAKILNFGRGNFAEQERNVADLDDGYARLSNMLLEAYSGAD
                     LTKRQFKVLLAILRKTYGWNKPMDRITDS"
     gene            complement(253..549)
                     /locus_tag="ACHOSC_RS22290"
                     /old_locus_tag="pgaptmp_004458"
     CDS             complement(253..549)
                     /locus_tag="ACHOSC_RS22290"
                     /old_locus_tag="pgaptmp_004458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CII family transcriptional regulator"
                     /protein_id="HGU4550257.1"
                     /translation="MEQTSYSKLSQREIDRAETDLLINLSTLTQRGLAKMIGCHESKI
                     SRTDWRFIASVLCAFGMASDISPISRAFKYALDEITKKKSPVAAGDSKQIDMQF"
     gene            complement(688..915)
                     /locus_tag="ACHOSC_RS22295"
                     /old_locus_tag="pgaptmp_004459"
     CDS             complement(688..915)
                     /locus_tag="ACHOSC_RS22295"
                     /old_locus_tag="pgaptmp_004459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001180318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HGU4550258.1"
                     /translation="MQNLDEPIKGVGIPEVAKACGVSERAVYKWLKNGFLPKTEFFGK
                     TKYASKIEEISGGKYQASEMLEISKKNLLAA"
     gene            994..1701
                     /locus_tag="ACHOSC_RS22300"
                     /old_locus_tag="pgaptmp_004460"
     CDS             994..1701
                     /locus_tag="ACHOSC_RS22300"
                     /old_locus_tag="pgaptmp_004460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000250468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S24 family peptidase"
                     /protein_id="HGU4550259.1"
                     /translation="MVQNEKVRKEFAQRLAQACKEAGLDEHGRGMAIARALSLSSKGV
                     SKWFNAESLPRQEKMNALAKFLNVDVVWLQHGTSLNGANDEDTLSFVGKLKKGLVRVV
                     GEAILGVDGAIEMTEERDGWLKIYSDDPDAFGLRVKGDSMWPRIKSGEYVLIEPNTKV
                     FPGDEVFVRTVEGHNMIKVLGYDRDGEYQFTSINQDHRPITLPYHQVAKVEYVAGILK
                     QSRHLDDIEAREWLKSS"
     gene            1871..2773
                     /locus_tag="ACHOSC_RS22305"
                     /old_locus_tag="pgaptmp_004461"
     CDS             1871..2773
                     /locus_tag="ACHOSC_RS22305"
                     /old_locus_tag="pgaptmp_004461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001133195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4550260.1"
                     /translation="MPNLPMGGSQGELPLVPTVEVVADGIPMGVLNDGTPYLTLYGLA
                     KLCGIDEAPLRMFTSNWETEKNKPRGQKVAAYLAEKGFNNVDRLYTRVINSSNVETHA
                     YPDYVCMAVLRYYALDATNFDRSVAIGNFVRLAEYTLKRMIYEKANYNPNAAIDVSFE
                     NYRARIKLNNQIPTTHFAVFREIADIAMTLIGNGFPMDDTTSLDGSVGNHWGKYWTKN
                     NLSERFGDRVQYQHIFPENYRQSAANKYIKAWIYPIESLGLFRKWLHDNYAMEKLPKY
                     LGNKKISNITELLEAVKKPALPNQ"
     gene            3600..3668
                     /locus_tag="ACHOSC_RS22310"
                     /old_locus_tag="pgaptmp_004462"
                     /pseudo
     CDS             3600..3668
                     /locus_tag="ACHOSC_RS22310"
                     /old_locus_tag="pgaptmp_004462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311021.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="antitermination protein"
BASE COUNT         1124 a          786 c          834 g         1003 t
ORIGIN      
        1 gagaatcggt gattctgtcc attggtttat tccacccata ggttttacgc agaatggcaa
       61 gcagcacttt aaactgtcgc ttggtcagat ctgcgcctga ataagcctca agcagcatat
      121 ttgatagtct ggcgtaacca tcatcgagat ctgccacatt acgctcctgt tcggcaaagt
      181 tacctctgcc gaagttgagt atttttgctg tatttgtcat aatgactcct gtggattgat
      241 ccagtaattc cctcagaatt gcatatcaat ttgcttagag tccccggcgg ccaccgggga
      301 ttttttcttt gtgatttcat caagcgcata cttaaaagct ctgctaatcg gactgatgtc
      361 tgatgccatg ccaaaagcac acaagaccga agctataaac ctccagtctg ttctgcttat
      421 cttcgattca tgacagccaa tcatctttgc cagaccgcgc tgggtaagcg ttgacaggtt
      481 gatgagtaaa tcagtttcag cgcgatcaat ttctcgctgt gatagtttgc tgtaacttgt
      541 ttgttccatt tcttaagatt tccaatagtg aatagctagt tgaaaggtat gcgtggaaac
      601 gcatatggcc ttagttggtc agatatattg ggactcgctt tgtcagcgac gtaggacgaa
      661 tgtccattgt gaaaatagcg gtgttactta tgcagccaga aggttctttt tgcttatttc
      721 aagcatttcg cttgcttgat atttgccacc agaaatctct tcgatttttg atgcgtattt
      781 agttttccca aaaaactcag tcttagggag gaagccgttt ttgagccact tatagacagc
      841 cctttcgcta actccacaag ccttcgcaac ttcagggatg ccgacacctt taatcggctc
      901 atcaagattt tgcataggaa tatccttttt cgtactttca gtacgtatta tggttgaact
      961 gaaagttttt gcaagtgctt tagtatcgta ctcatggttc agaatgaaaa agtgcgcaaa
     1021 gaattcgccc agcggctagc gcaagcctgt aaagaagctg gtcttgatga acatggtagg
     1081 ggaatggcta tagcccgtgc cctttctctt tcgtccaaag gcgttagcaa atggtttaat
     1141 gctgagtctt taccgcgtca ggaaaaaatg aatgcgcttg cgaaatttct aaacgttgat
     1201 gttgtttggc ttcagcacgg cacttcgtta aatggagcga atgatgaaga tactctttca
     1261 tttgttggca aattaaaaaa agggttagtg cgcgtggttg gtgaggcaat tcttggtgtt
     1321 gatggtgcca tcgagatgac cgaagagcgc gatgggtggc tcaaaattta tagcgatgat
     1381 ccagatgcct ttggtcttcg tgtgaaagga gacagcatgt ggcccagaat aaaatcagga
     1441 gaatatgtac tcattgagcc taacaccaaa gtattcccgg gggatgaggt gtttgtcaga
     1501 actgttgaag gacacaacat gatcaaagtt cttggctatg acagagacgg agaataccaa
     1561 tttacaagca tcaaccaaga tcataggcca ataacgttgc cttatcatca agtagcaaag
     1621 gtggagtatg tagctggtat tctgaagcaa tctcgccatc tggatgacat cgaggcaagg
     1681 gagtggctga aaagttcgtg acttcatcgt cacatagctg gtaaccagtg gcctgaagag
     1741 acgtttgggt aaccacaata caaccttgtt gtcgcaaaaa gatcgcatat gcgattgttt
     1801 attagaaata gaaatcgcca tataggcgaa tagtcgatcg cggcgcataa cctaaggaaa
     1861 tcaataacat atgccaaatt taccgatggg cggatcgcag ggtgaattac ccttagtacc
     1921 tacagtagaa gttgttgctg atggcattcc tatgggggtg cttaatgatg gaacccctta
     1981 tctaactctt tacggtttag ccaaattatg tggcatagat gaagcacctc ttcgaatgtt
     2041 tacatctaac tgggagactg aaaaaaataa gccaagagga caaaaagtag ctgcatactt
     2101 agctgagaaa ggatttaaca acgttgaccg actgtatacc cgcgtcataa atagctcaaa
     2161 cgttgaaaca catgcttatc ctgactatgt ttgtatggct gttcttaggt actatgccct
     2221 cgatgccaca aattttgata gatccgttgc aataggaaac tttgttcgct tagctgaata
     2281 cacgctcaag cgaatgatct atgaaaaagc aaactataac cccaacgctg ccatagacgt
     2341 ttcattcgaa aactatagag ctcgtataaa gctaaacaac caaataccaa caactcattt
     2401 tgctgtattc agagaaatag cagatattgc aatgacgtta ataggaaatg gctttcctat
     2461 ggatgatacc acgtcactag acggaagcgt tggaaatcac tggggaaagt attggacaaa
     2521 aaacaacctt tccgagagat ttggtgatag agttcaatat caacacattt tccctgaaaa
     2581 ctacaggcaa tctgcagcca acaagtacat aaaagcctgg atttacccta tcgaatcatt
     2641 ggggctattc aggaaatggc ttcacgataa ctatgccatg gaaaaactac ccaaatatct
     2701 tggaaacaaa aaaatcagta acataactga actattggaa gccgtcaaaa aaccagcatt
     2761 accaaaccag tgataaaatt atttacgcta cagcaacccg gactctgcgc cgggttttct
     2821 ttgcctcacg ttcgcccacc taaaaaacat aaccaattgt atttattgat gtaactcgct
     2881 aaaccatgca gttatgatcc ctgccgcata accttcatca gccacatttt caaaaataaa
     2941 tttccttata tatcagaatc atacttcgta gagttaataa atcaccaaaa ttcgtaccaa
     3001 tagttcttga taatgtcgaa ctattggttc attattatcg tcgtcagcag gacgcattac
     3061 tcaccagggc ggtgaatata caacgattcg aatatgaatc tacggcgctg acaaagcgca
     3121 ataaccaaag tgaactttgg ggtgtggtga agggttcatg gacgggaata tgtcgcacgt
     3181 aaagcggcga ggcctgcggg actattgccg aattgaagta ggccgaaaca ggtcgaaatg
     3241 ggtctcccac ctaccacacc accaaagttc atcaggaggt ctatatgaca cgcagaactc
     3301 agttcaaagg caattcacgt tctcgtcgtc gtgagcgttt aaaggcaaag gcattagcta
     3361 acggcgtact ggcccgcgaa gaagcaataa gttcagaagt attacaccgc ccaactctaa
     3421 gtagagcgca gattcaggct aaaggtactc acgaaacgcc tgatcgcata gaagacgcta
     3481 agccaattaa gttcatggca caggacgtga tctggcagca ggaagaatac agacgcaatc
     3541 tggagcgagc ggccattgtg tacgcgaatg agtttggaca taagcaacca gaaactggtg
     3601 tatgtcttcc aaatgtagct ctttacgcgg caggctaccg gaaatccaaa caactgacag
     3661 caagatgact tgtgttggtc gccagaaaat gaaattaggc agcaaaccac ttatttgagg
     3721 tgagatatga caaaatcatg gagcgta
//
LOCUS       DAZKHF020000327         3730 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2201S31241
            SAMN43267800-rid25106413.denovo.329, whole genome shotgun sequence.
ACCESSION   DAZKHF020000327 DAZKHF020000000
VERSION     DAZKHF020000327.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267800
            Sequence Read Archive: SRR30307263
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3730)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3730)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 3730)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350581.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350581.2
            Genome Coverage       :: 151x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:21:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,652
            CDSs (total)                      :: 5,557
            Genes (coding)                    :: 5,320
            CDSs (with protein)               :: 5,320
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 237
            CDSs (without protein)            :: 237
            Pseudo Genes (ambiguous residues) :: 0 of 237
            Pseudo Genes (frameshifted)       :: 102 of 237
            Pseudo Genes (incomplete)         :: 130 of 237
            Pseudo Genes (internal stop)      :: 52 of 237
            Pseudo Genes (multiple problems)  :: 41 of 237
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3730
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267800-rid25106413.denovo.329"
                     /serotype="O 26:H11"
                     /isolate="2201S31241"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-01"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            66..239
                     /gene="ymgI"
                     /locus_tag="ACHOSC_RS22315"
                     /old_locus_tag="pgaptmp_004463"
     CDS             66..239
                     /gene="ymgI"
                     /locus_tag="ACHOSC_RS22315"
                     /old_locus_tag="pgaptmp_004463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YmgI"
                     /protein_id="HGU4550261.1"
                     /translation="MSYSSFKIILIHVKNIIPIITATLILNYLNNSERSLVKQILIED
                     EIIVCATYLIPDI"
     gene            241..585
                     /locus_tag="ACHOSC_RS22320"
                     /old_locus_tag="pgaptmp_004464"
     CDS             241..585
                     /locus_tag="ACHOSC_RS22320"
                     /old_locus_tag="pgaptmp_004464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415690.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YMGG-like glycine zipper-containing protein"
                     /protein_id="HGU4550262.1"
                     /translation="MKKKILAFGLISALFCSTPAMADMNRTTKGALLGAGVGLLTGNG
                     VNGVLKGAAVGAGVGAVTEKGRDGKNARKGAKVGAAVGAVTGVLTGNGLEGAIKGAVI
                     GGTGGAILGKMK"
     gene            589..924
                     /locus_tag="ACHOSC_RS22325"
                     /old_locus_tag="pgaptmp_004465"
     CDS             589..924
                     /locus_tag="ACHOSC_RS22325"
                     /old_locus_tag="pgaptmp_004465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YmgD family protein"
                     /protein_id="HGU4550263.1"
                     /translation="MMMKKFALLAGLFVFAPMTWAQDYNIKNGLPSETYITCAEANEM
                     AKTDSAQVAEIVAVMGNASVASRDLKIEQSPELSAKVVEKLNQVCAKDPQMLLITAID
                     DTMRAIGKK"
     gene            complement(981..3302)
                     /locus_tag="ACHOSC_RS22330"
                     /old_locus_tag="pgaptmp_004466"
     CDS             complement(981..3302)
                     /locus_tag="ACHOSC_RS22330"
                     /old_locus_tag="pgaptmp_004466"
                     /inference="COORDINATES: protein motif:HMM:TIGR01414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter outer membrane beta-barrel
                     domain-containing protein"
                     /protein_id="HGU4550264.1"
                     /translation="MQGKNTIVITGDYSIGLLSQTSGNLNTDTIIRVNSDGSVTPSFS
                     DGDDTFIVTAGNHAVGVLACASPGSARACVSSLDEESTTDTGSNENNAIAKLDMAKGE
                     ITTHGTESYAAYANGTVVKAGDTLDYTNASVTLTDVDITTHGDNAHAIAARQGTVSFN
                     QGEIYTTGPDAATAKIYNGGTVTLKNTSAVAHQGSGIVLESSINGQEATVDILSGSSL
                     GSANEILYHKNETSNVTITDSEVSSAADVFINNIKGHLTVDATNSKITGSANISTDVN
                     THTYLSLSDNSTWDIKADSTVSNLTVDNSTVYISRADGRDVEPTRLTITENYVGNNGV
                     LHLRTELGDDNSATDKVVINGNTSGTTRVKVTNAGGSGAYTLNGIEIISVEGESNGEF
                     IKDSRIFAGAYEYSLTRGNTEATNKNWYLTNFQATSGGETNSGGSSAPTVAPTPVLRL
                     EAGSYVANLAAANTLFVMRLNDRAGEMRYIDPVTEQERSSRLWLRQIGGHNAWRDSNG
                     QLRTTSHRYVSQLGAELLTGGFTDSDSWRLGVMAGYARDYNSTHSSVSDYRSKGSVRG
                     YCAGLYATWFADDISKKGAYIDAWAQYSWFKNSVKGDELAYESYSAKGATVSLEAGYG
                     FALNKSFGLEAAKYTWIFQPQAQAIWMGVDHNAHTEANGSRIENDANNNIQTRLGFRT
                     FIRTQEKNSGPHGDDFEPFVEMNWIHNSKDFAVSMNGVKVEQDGASNLGEIKLGVNGN
                     LNPAASVWGNVGVQLGDNGYNDTAVMVGLKYKF"
     gene            complement(3419..3628)
                     /locus_tag="ACHOSC_RS22335"
                     /old_locus_tag="pgaptmp_004467"
     CDS             complement(3419..3628)
                     /locus_tag="ACHOSC_RS22335"
                     /old_locus_tag="pgaptmp_004467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001297677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4550265.1"
                     /translation="MKLKKLSGFSLGLIALAVGNAYAEQFIQHDEYKHAVAIKDGKVK
                     DIDNALVITTGNGSYGYPLQERVAS"
BASE COUNT          988 a          895 c          804 g         1043 t
ORIGIN      
        1 atcttacctt tacaacattg cgttaacagt caatgacact tcttgaataa aaatattgtc
       61 aatcaatgag ttattcctca tttaagatta ttctcattca cgtaaaaaac ataattccta
      121 tcatcacagc cacgctcatt ttgaattatc tgaataatag cgagcgttct ttagttaagc
      181 agatcttaat tgaagacgaa attattgtgt gtgcaactta cttaataccg gacatctaag
      241 ttgaagaaaa aaatacttgc cttcgggctt atctcagcat tgttctgctc cacgccagcg
      301 atggcggata tgaatcgcac cacgaaaggt gcattgctag gcgcaggcgt tggtttactt
      361 actggcaatg gtgttaatgg cgtactgaaa ggtgcagctg ttggcgctgg tgttggtgca
      421 gtaacagaaa aaggccgcga cggtaaaaat gcgcgtaaag gtgcaaaagt aggtgccgcc
      481 gttggtgctg tcactggcgt tctgaccggc aatggcctcg aaggggcgat taaaggtgcc
      541 gtgattggcg gtacgggtgg tgcaattctg ggtaaaatga aataaggaat gatgatgaaa
      601 aaatttgctt tactggcggg cctgtttgtt ttcgcaccaa tgacctgggc gcaggactac
      661 aatattaaaa acggtttgcc gtcagaaacc tatattactt gcgcagaagc caacgaaatg
      721 gcaaaaactg acagcgcaca ggttgcagaa attgttgcgg taatgggtaa tgccagcgtt
      781 gccagccgtg atttaaaaat tgagcaatct ccggaactga gcgccaaagt ggtagaaaaa
      841 ctgaatcagg tttgcgctaa agacccgcaa atgctgttga tcacggcaat tgatgacacc
      901 atgcgtgcta tcggcaaaaa ataatatttc atatgtaccg cgtcgatgaa tacgacgcgg
      961 ttttttagct aacggcggga tcagaactta tatttcaggc ccaccatcac tgcggtgtca
     1021 ttgtagccat tatcacccag ctgcacgccc acattccccc agacgctggc cgctggattc
     1081 aggttgccat ttacgccaag tttaatttcc cccaaattac tcgccccatc ttgttcgact
     1141 ttcacaccgt tcattgagac agcaaaatct ttactgttat ggatccagtt catttcaaca
     1201 aaaggttcaa agtcgtcacc gtgcggaccg ctgtttttct cctgagtacg aataaaggtg
     1261 cggaaaccaa gtcgggtctg gatgttgtta tttgcgtcat tctcaatacg tgagccattg
     1321 gcttcggtgt gcgcattatg atcgacgccc atccagatag cctgtgcctg tggctggaat
     1381 atccacgtgt atttcgccgc ttccagacca aaggatttat tcagggcaaa gccgtaaccc
     1441 gcttccagcg aaacggttgc gcctttcgca ctataggatt cataggctaa ttcatcccct
     1501 ttcaccgagt ttttaaacca gctatattgc gcccaggcgt caatgtatgc gcctttttta
     1561 ctgatgtcat cggcaaacca agtggcatac agccctgcgc aatagcctct gacactccct
     1621 ttcgaacgat aatccgacac gctggaatga gttgagttgt agtcgcgggc ataaccagcc
     1681 atcactccca aacgccaact gtcactgtcg gtaaaaccac cggttaacag ctcggcccct
     1741 aactgcgaga cgtagcgatg cgaggtcgtt ctcaactgtc cgttgctgtc acgccaggca
     1801 ttatgcccgc caatttgacg tagccaaagt cggcttgaac gctcctgttc agttacagga
     1861 tcgatgtagc gcatttcacc cgcacggtcg ttcagacgca taacaaaaag agtgttagcg
     1921 gctgccaggt tggcgacgta acttccagct tcgaggcgca ggacgggggt aggcgcaaca
     1981 gtaggcgctg aacttcctcc ggagtttgtt tcaccgccgc tcgttgcctg gaagttagtc
     2041 agataccagt ttttattggt cgcttcggta ttacctcggg ttaatgaata ttcgtaggca
     2101 ccggcgaaaa tcctcgaatc cttaataaat tccccatttg attccccctc aacgctgata
     2161 atctctatcc catttaacgt gtaagctccg ctgcctcctg cattggtaac tttaactcgg
     2221 gttgttccag aggtatttcc attaataacg actttatccg tagctgaatt atcatcaccc
     2281 aattcagttc tgagatgcaa tacgccatta ttaccaacgt aattttcagt tattgttaat
     2341 cttgttggtt cgacatcccg tccatctgcc cgggaaatat agaccgtact attatcaacc
     2401 gtgaggttgc tcaccgttga gtcagcttta atatcccagg tactattatc tgaaagcgac
     2461 agataggtat gagtgttaac atctgttgaa atattagctg aacccgttat ttttgaatta
     2521 gttgcatcga cggtcaaatg acctttaata ttattgataa aaacatctgc agccgatgat
     2581 acctcactat ccgtaatggt cacgttactc gtctcatttt tatggtagag gatttcattt
     2641 gctgacccta gtgagctacc agataaaata tctaccgttg cttcctgacc atttatggaa
     2701 gactccagca caattccaga cccctgatgc gcgactgcag atgtattttt cagcgtcacc
     2761 gtgccgccat tataaatttt agcagtcgcg gcgtcaggac ccgttgtata aatttctccc
     2821 tggttaaatg aaactgtccc ctggcgggca gcgatggcat gagcattgtc cccatgcgtg
     2881 gtgatatcaa catcggttaa tgtaacgctg gcattggtat aatcaagcgt gtcaccagcc
     2941 ttgacgacag tgccattagc gtacgcggca taactttctg ttccgtgggt tgtgatctcc
     3001 ccttttgcca tatccagttt tgctatggca ttattttcat tacttcctgt atcggtagta
     3061 ctttcttcat caagagaaga tacacacgca cgcgcgcttc cgggagatgc acacgcaaga
     3121 acgccgactg catgattacc cgcagtaaca ataaatgtat catctccgtc agaaaaagag
     3181 ggggtaaccg aaccgtcaga gttgactctt attatggtgt cagtattcag gtttccactc
     3241 gtttgactta gcaaaccaat tgagtaatcc cccgttatga caatcgtgtt tttaccctgc
     3301 aaaataacag caccgccaaa ttcacttacg attgcgctag ctgttaatgc tgtgcttaga
     3361 ggactattgt cagaaggata taaaccgccc gttgttacaa tgacgccatt gttaatggtt
     3421 aacttgctac cctctcctgc aacggatacc cgtaacttcc attacccgtt gttataacta
     3481 gcgcattatc aatatctttt acttttccat cttttattgc cacagcatgt ttatattcat
     3541 cgtgctgaat aaactgttca gcatatgcat tacccaccgc cagagcaata agtcccaaac
     3601 taaacccgga gagttttttc agtttcatag attaaccatc cattcattgg attaacaaac
     3661 attcctggtc aaataaccta tgcgttcaga ttaccaaaac ggagggtaaa gacaaaaaca
     3721 aaagcacaaa
//
LOCUS       DAZKHF020000328         3720 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2201S31241
            SAMN43267800-rid25106413.denovo.330, whole genome shotgun sequence.
ACCESSION   DAZKHF020000328 DAZKHF020000000
VERSION     DAZKHF020000328.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267800
            Sequence Read Archive: SRR30307263
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3720)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3720)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 3720)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350581.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350581.2
            Genome Coverage       :: 151x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:21:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,652
            CDSs (total)                      :: 5,557
            Genes (coding)                    :: 5,320
            CDSs (with protein)               :: 5,320
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 237
            CDSs (without protein)            :: 237
            Pseudo Genes (ambiguous residues) :: 0 of 237
            Pseudo Genes (frameshifted)       :: 102 of 237
            Pseudo Genes (incomplete)         :: 130 of 237
            Pseudo Genes (internal stop)      :: 52 of 237
            Pseudo Genes (multiple problems)  :: 41 of 237
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3720
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267800-rid25106413.denovo.330"
                     /serotype="O 26:H11"
                     /isolate="2201S31241"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-01"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            complement(60..1508)
                     /gene="cusS"
                     /locus_tag="ACHOSC_RS22340"
                     /old_locus_tag="pgaptmp_004468"
     CDS             complement(60..1508)
                     /gene="cusS"
                     /locus_tag="ACHOSC_RS22340"
                     /old_locus_tag="pgaptmp_004468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001739168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cu(+)/Ag(+) sensor histidine kinase CusS"
                     /protein_id="HGU4550266.1"
                     /translation="MVSKPFQRPFSLATRLTFFISLATIAAFFAFAWIMIHSVKVHFA
                     EQDINDLKEISATLERVLNHPDETQARRLMTLEDIVSGYSNVLISLADSHGKTVYHSP
                     GAPDIREFARDAIPDKDARGGEVYLLSGPTIMMPGHGHGHMEHSNWRMINLSVGPLVD
                     GKPIYTLYIALSIDFHLHYINDLMNKLIMTASVISILIVFIVLLAVHKGHAPIRSVSR
                     QIQNITSKDLDVRLDPQTVPIELEQLVLSFNHMIERIEDVFTRQSNFSADIAHEIRTP
                     ITNLITQTEIALSQSRSQKELEDVLYSNLEELTRMAKMVSDMLFLAQADNNQLIPEKK
                     MLNLADEVGKVFDFFEALAEDRGVELRFVGDECQVAGDPLMLRRALSNLLSNALRYTP
                     PGEAIVVRCQTVDHQVQVTVENPGTPIAPEHLPRLFDRFYRVDPSRQRKGEGSGIGLA
                     IVKSIVVAHKGTVAVTSDARGTRFVIMLPERE"
     gene            complement(1498..2181)
                     /gene="cusR"
                     /locus_tag="ACHOSC_RS22345"
                     /old_locus_tag="pgaptmp_004469"
     CDS             complement(1498..2181)
                     /gene="cusR"
                     /locus_tag="ACHOSC_RS22345"
                     /old_locus_tag="pgaptmp_004469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper response regulator transcription factor
                     CusR"
                     /protein_id="HGU4550267.1"
                     /translation="MKLLIVEDEKKTGEYLTKGLTEAGFVVDLADNGLNGYHLAMTGD
                     YDLIILDIMLPDVNGWDIVRMLRSANKGMPILLLTALGTIEHRVKGLELGADDYLVKP
                     FAFAELLARVRTLLRRGAAVIIESQFQVADLMVDLVSRKVTRSGTRITLTSKEFTLLE
                     FFLRHQGEVLPRSLIASQVWDMNFDSDTNAIDVAVKRLRGKIDNDFEPKLIQTVRGVG
                     YMLEVPDGQ"
     gene            2338..3711
                     /gene="cusC"
                     /locus_tag="ACHOSC_RS22350"
                     /old_locus_tag="pgaptmp_004470"
     CDS             2338..3711
                     /gene="cusC"
                     /locus_tag="ACHOSC_RS22350"
                     /old_locus_tag="pgaptmp_004470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cu(+)/Ag(+) efflux RND transporter outer
                     membrane channel CusC"
                     /protein_id="HGU4550268.1"
                     /translation="MSPCKLLPFCVALALTGCSLAPDYQRPAMPVPQQFSLSQNGLVN
                     AADNYQNVGWRTFFVDNQVKTLISEALVNNRDLRMATLKVQETRAQYRLTDADRYPQL
                     NGEGSGSWSGNLKGNTATTREFSTGLNASFDLDFFGRLKNMSEAERQNYLATEEAQRA
                     VHILLVSNVAQSYFNQQLAYAQLQIAEETLRNYQQSYAFVEKQLLTGSSNVLALEQAR
                     GVIESTRSDIAKRQGELAQANNALQLLLGSYGKLPQAQTVNSDSLQSVKLPAGLSSQI
                     LLQRPDIMEAEHALMAANANIGAARAAFFPSIRLTSGISTASSDLSSLFNASSGMWNF
                     IPKIEIPIFNAGRNQANLDIAEIRQQQSVVNYEQKIQNAFKEVADALALRQSLNDQIS
                     AQQRYLASLQITLQRARALYQHGAVSYLEVLDAERSLFATRQTLLDLNYARQVNEISL
                     YTALGGG"
BASE COUNT          924 a          968 c          960 g          868 t
ORIGIN      
        1 atttgttgat caacaatacc aggaggtgat gcaccacgcc ctgaatttga acttaataat
       61 cactctctct ccggcagcat aatcacaaac cttgtccccc gcgcatctga cgttactgca
      121 accgtgcctt tatgcgcgac aacaatcgat ttcactatcg ccagcccaat gccgctacct
      181 tcgccttttc gctggcggga agggtcaacg cggtagaaac ggtcaaacaa tcgcggtaag
      241 tgctcgggcg caatgggcgt accggggttt tcgacggtaa cttgcacctg gtgatcgacc
      301 gtctggcagc gcactacaat tgcctctccg ggcggcgtat aacgcagggc attagaaagc
      361 aggttgctta acgcccgacg cagcatcagc ggatcgcccg caacctgaca ttcgtcgcca
      421 acaaatcgca actccacgcc gcgatcttcc gctaacgcct cgaaaaaatc gaacactttg
      481 ccgacttcat ccgccaggtt gagcattttc ttttcgggga ttagctggtt gttatcggcc
      541 tgagcgagaa acagcatatc gctgaccatt ttcgccattc gcgtcagctc ttcgagatta
      601 gagtagagca catcttccag ctccttctgg ctgcgagact ggctgagggc gatttccgtt
      661 tgcgtgatga gattggtaat cggcgtgcga atttcgtgag cgatatccgc tgagaaattg
      721 gactggcggg taaagacatc ctcaatacgc tcgatcatat ggttgaacga cagtaccagc
      781 tgttccagct caatgggcac ggtctgcggg tcaaggcgaa cgtcgagatc tttcgaggtg
      841 atattctgga tttgacggct gacgctgcgg atcggcgcgt gacctttatg taccgccaac
      901 agtacgataa agacgatcag gatgctgatt accgatgcgg tcataataag tttattcatc
      961 aaatcattta tgtaatgaag atgaaaatcg atcgaaagcg cgatgtagag cgtataaatc
     1021 ggtttgccgt ccaccaacgg gccaaccgac aagttaatca tccgccagtt gctgtgttcc
     1081 atatgcccgt gaccgtggcc tggcatcata atcgtcgggc cggaaaggag atacacctcg
     1141 ccaccccgcg cgtctttatc gggtatggca tcacgtgcaa actcgcggat atcaggcgca
     1201 ccgggggagt gatacaccgt tttgccgtgg ctatctgcca gggagatcaa cacgttggaa
     1261 taaccactga cgatatcttc cagcgtcatt aagcgtcggg cttgcgtctc gtcaggatga
     1321 ttaagtaccc gttcaagcgt ggcgctaatt tcttttaaat cattaatatc ctgctcggca
     1381 aaatgcactt ttactgagtg gatcatgatc catgcaaagg cgaaaaacgc tgcgatggtg
     1441 gccaggctga taaaaaaggt caggcgggtt gccagcgaaa acgggcgctg aaatggctta
     1501 ctgaccatcc ggcacctcaa gcatgtaacc cacgccgcgc acggtctgaa ttagctttgg
     1561 ctcaaagtcg ttgtcgattt tgccgcgcag ccgcttcacc gctacatcaa tggcgttagt
     1621 gtcgctgtcg aaattcatgt cccacacctg cgaggcgata agcgagcggg gcagcacttc
     1681 gccctgatgg cggaggaaaa actccagcag agtaaactct ttactggtca gagtgatgcg
     1741 cgtgccgctg cgggtgactt tgcggctgac gagatcgacc atcagatcgg caacctgaaa
     1801 ctgactttcg ataatcaccg ccgccccgcg ccgcaggaga gtgcgcaccc gcgccagcag
     1861 ttcagcaaaa gcgaatggtt tcaccaggta gtcatctgcc cccaactcca gccccttgac
     1921 gcgatgttca atggtgccaa gcgcggtaag caacagaatc ggcatccctt tattggcgga
     1981 acgcaacatg cgcacgatat cccagccgtt cacgtccggc agcataatat cgaggattat
     2041 cagatcataa tcaccggtca tcgccagatg gtagccattc agcccgttgt cggccaaatc
     2101 gaccacaaaa ccggcttcgg ttaacccttt ggtcaagtat tctccggttt tcttttcatc
     2161 ttcgacaatc aacagtttca tttttcctcc gcatgttgcc cgggcaattc tagagtagcg
     2221 ggatcagatg gcaatcgctt attggcaaaa tgacaatttt gtcattttcc tgtcaccgga
     2281 aaatcagagc ctggcgagta aagttggcgg tataaaatca ccagaaatta tgagcctatg
     2341 tctccttgta aacttctgcc attttgtgtg gcccttgcgc taaccggttg ttcactggca
     2401 ccggattatc agcgtccggc aatgcccgtg ccgcagcagt tctcactcag ccagaacggc
     2461 ttggttaacg cagcagataa ctaccagaac gtgggctggc gcaccttttt tgttgataat
     2521 caggtaaaga cgctgattag cgaggcgctg gtgaataacc gggatttgcg catggcgacg
     2581 ctgaaagtgc aggaaacgcg ggcgcaatat cgtctgaccg atgccgaccg ctacccacag
     2641 ctcaatggcg agggcagcgg cagctggagc ggtaatctta aagggaatac cgccacgaca
     2701 cgggagtttt cgaccggcct taacgccagc tttgatctcg attttttcgg tcgcttaaag
     2761 aacatgagcg aagccgagcg ccaaaattat ttagccactg aggaagccca gcgcgcggtg
     2821 catattttgc tggtttctaa tgtcgcgcaa agttatttca atcagcagct agcgtatgcg
     2881 caattgcaaa tagccgaaga aacgctgcgt aattatcagc agtcatatgc gtttgtcgaa
     2941 aaacaactgt tgacaggtag cagtaatgtt ctggcgctgg aacaggctcg cggggtgatt
     3001 gaaagtaccc gcagcgacat cgctaaacgt cagggggaac tggcgcaggc gaataatgca
     3061 ttgcaactgt tattgggaag ctacggcaag ctgccgcaag cgcagacagt aaacagcgac
     3121 agcctgcaaa gcgttaaatt accggcggga ttgtcgtcgc aaatcttatt gcagcgcccg
     3181 gatattatgg aagctgaaca cgcgttaatg gcggctaatg ccaatattgg ggccgcacgc
     3241 gcggcatttt tcccatctat aaggctgacc agcggaatat caaccgccag cagcgatctg
     3301 tcgtcattat ttaacgccag cagcgggatg tggaatttta ttcctaaaat tgagatcccc
     3361 atttttaatg ccggacgcaa ccaggccaat ctggatatcg ccgaaattcg ccagcagcag
     3421 tcggtagtga attatgaaca gaaaatccag aacgccttta aagaagtggc agatgcgctg
     3481 gcattacgtc aaagcctgaa cgatcaaatc agcgcccagc agcgttatct ggcgtcgctg
     3541 caaattactt tgcaacgggc gcgggcatta tatcagcacg gcgcagtaag ttatctggaa
     3601 gtgctggatg ccgagcgttc tttatttgca acccgacaaa ctttactcga cctgaattat
     3661 gcccgtcagg ttaacgaaat ttctttgtat accgccttag gtggcggttg acagcaatat
//
LOCUS       DAZKHF020000329         3700 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2201S31241
            SAMN43267800-rid25106413.denovo.331, whole genome shotgun sequence.
ACCESSION   DAZKHF020000329 DAZKHF020000000
VERSION     DAZKHF020000329.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267800
            Sequence Read Archive: SRR30307263
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3700)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3700)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 3700)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350581.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350581.2
            Genome Coverage       :: 151x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:21:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,652
            CDSs (total)                      :: 5,557
            Genes (coding)                    :: 5,320
            CDSs (with protein)               :: 5,320
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 237
            CDSs (without protein)            :: 237
            Pseudo Genes (ambiguous residues) :: 0 of 237
            Pseudo Genes (frameshifted)       :: 102 of 237
            Pseudo Genes (incomplete)         :: 130 of 237
            Pseudo Genes (internal stop)      :: 52 of 237
            Pseudo Genes (multiple problems)  :: 41 of 237
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3700
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267800-rid25106413.denovo.331"
                     /serotype="O 26:H11"
                     /isolate="2201S31241"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-01"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            <1..698
                     /locus_tag="ACHOSC_RS22355"
                     /old_locus_tag="pgaptmp_004471"
     CDS             <1..698
                     /locus_tag="ACHOSC_RS22355"
                     /old_locus_tag="pgaptmp_004471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000039778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="inverse autotransporter beta domain-containing
                     protein"
                     /protein_id="HGU4550269.1"
                     /translation="TITEAVEITTKAIKQKITPSDIYRHALSGNILLLVYFQSPVILK
                     KIQTFNGKIKFRQFVGDLLDKLCMLDRDGFIYGQNLRLCTEARYICPVQQIIDTPLLR
                     KLNQFRTFVRNVRPGDELDVQAQVSEKNLTPPPGNSSGNLEQQIASTSQLIGSLLAED
                     MNSEQAANIARGWASSQTSGVMTDWLSRFGTARITLGVDEDFSLKNSRDGNPRKWRFD
                     ATNSELCAERHEC"
     gene            625..2747
                     /locus_tag="ACHOSC_RS22360"
                     /old_locus_tag="pgaptmp_004472"
                     /pseudo
     CDS             625..2747
                     /locus_tag="ACHOSC_RS22360"
                     /old_locus_tag="pgaptmp_004472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416485.4"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing N-terminus and C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="Ig-like domain-containing protein"
     gene            complement(2917..>3700)
                     /locus_tag="ACHOSC_RS22365"
                     /old_locus_tag="pgaptmp_004473"
     CDS             complement(2917..>3700)
                     /locus_tag="ACHOSC_RS22365"
                     /old_locus_tag="pgaptmp_004473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="glycosyltransferase family 9 protein"
                     /protein_id="HGU4550270.1"
                     /translation="HATLKEIKDLAKNITLQYGTPDLCIEAMRKKNLKTMIFISQLKA
                     KTNFQVVGLTMKCYSPLCKNASRMDQNLRTPVPMTWAFMMREAGFPAVRSIYELPLSD
                     DVLDEVREEMRSLGSYIALNLDGSSQERTFSLSIAENLIAKIQSETDIPIVIVYGPKG
                     EDKARALVDCYNNVYRLSLSPSIKRSAAIIKDAYIAITPDTSILHMASAYNTPVVAIY
                     ADYKTRWPAMADVSESVVVGQKIDNISLDEFAKALKSVLARI"
BASE COUNT         1078 a          856 c          846 g          920 t
ORIGIN      
        1 tcactataac ggaagcagtt gagatcacta ccaaagccat aaaacaaaaa attactccta
       61 gcgatattta tcgtcatgcc ttgagtggca acatcctact attggtctat ttccagtctc
      121 ctgtgatact taagaagata caaactttta atggaaaaat aaaattcagg cagtttgtgg
      181 gagacctact tgataaacta tgcatgcttg acagggacgg ttttatttat ggtcaaaatt
      241 taagactctg tacagaagcc agatatattt gtcctgtgca acaaattata gatacccccc
      301 tgttacgcaa actcaaccag tttcgtacgt ttgttcgtaa tgtccgcccg ggtgatgaac
      361 tggatgtcca ggcacaagtt agtgaaaaaa atttaacccc gccgccgggt aacagcagtg
      421 gcaaccttga gcaacagata gccagtactt cacagctaat tggatctctg ctcgccgaag
      481 atatgaacag cgagcaagcg gcaaacatag cacgtggatg ggcctcttct cagacttcag
      541 gggtaatgac agactggtta agccgcttcg gtaccgcaag aatcacgctg ggcgtggatg
      601 aagatttcag cctgaagaac tcccgtgacg gcaaccctcg aaaatggcga ttcgatgcaa
      661 caaacagtga actatgtgcc gaacgtcacg aatgctgaaa tcacgctggc agccttgaag
      721 gatccggtga ttgccgacaa taacgatctc acgacactaa cagcaccgtc gctgatacag
      781 agggcaatgc gatagccaac actgaggtaa catttactct accggaagat gtgaaggcga
      841 acttcacgct gagcgatggc gacaaagcgg ttactgatgc cgatggcaaa gcgaaagtca
      901 cgctgaaagg tacaaaagca ggcgctcata ccgtgacagc atcgatggtt ggcggtaaga
      961 gtgagcagtt agtggtgaac tttactgcgg atacgctcac tgcgcaggtt aatcttaacg
     1021 ttaccgagga caatttcatc gccaataaca ttggaatgac caggctacaa gcaatagtga
     1081 ctgatggaaa cggcaaccca gtagaaggta taaaagttaa tttccgtgga acctccgtca
     1141 cgctaagcag caccagcgtt gaaacggatg atcaggtttt tgctgaaatt cttgtgacaa
     1201 gcaccgaggt cggactaaaa acagtttcag cctctctggc agataaacct actgaagtca
     1261 tctcgcgatt actgaatgcc aaggtagatg ttaattctgc gacgattacc agtcaggaga
     1321 tacctgaagg tcaggtcatg gtcgcacaag acatagcagt taaagctcac gttaacgacc
     1381 agtttggcaa cccggtgacg catcaagccg cgacattcag tgcagcgcca tcctcgcaaa
     1441 tgatcatcag ccagaacacg gtctctacta atacgcaggg agtagccgag gtcaccatga
     1501 cgtctgaaag aaacggctcg tatactgtaa aagcatccct agcgaatgga gcttcacttg
     1561 agaaacaact ggaggctatt gatgaaaaac tgacactcac gtccagtccg cttatcggtg
     1621 tcaatgctcc taaaggcgct actctgacgg caacgctaac ctctgcaaat ggcactccag
     1681 tggagggtca ggtcatcaac tttagcgtaa cgctagaagg tgcgacgtta agtggcggaa
     1741 aagtgagaac caactcttca ggtcaggctc cagtcgttct gaccagcaat aaagtcggta
     1801 catatacggt gactgcatcg ttccataacg gcgtcacaat acagacacag acaaccgtga
     1861 aggttactgg caacccaagc accgcacatg ttgctagctt tatcgctgat ccgtcgacta
     1921 tcgccgccac caacagtgat ttaagtacct taaaggcaac ggttgaggat ggcagtggta
     1981 acctgatcga aggtctcact gtgtacttcg ccttaaaaag cggctccacc acattaacgt
     2041 cattaacagc ggtgaccgat caaaacggaa tcgcgacaac aaacgtgaga tgagcgataa
     2101 cagggagcgt cactgtaagc gcagtcataa gcgcaggtgg aatgcaaaca gtagatatat
     2161 cgctagtggc aggcccagca gacgcctcgc agtccatcct taagaacaac cagtcatcac
     2221 tgaaagggga ctttaccgat agtgctgagc tacatcttgt tctgcacgat atatcaggca
     2281 atccgatcaa agtttctgaa gggatggaat ttgtgcaatc aggtacaaac gtgccctata
     2341 tgaaaattag cgcaattgat tacagtcaaa atatcaatgg tgattacaaa gctactgtta
     2401 caggagacgg agagggtatc gcaacgctga tccccgtatt gaatggtgtt catcaggcgg
     2461 gcctgagtac cacgatagaa ttcattagcg cggaaaccag accaatgacg ggtacagtgt
     2521 cagtgaatag tgcaaaccta ccgacagctt cattcccttc gcagggattt accggggcgt
     2581 attatcagtt gaataatgac aactttgcac caggaaaaac ggcggctgat tattcgtttt
     2641 caagctcggc ctcctgggta ggcgttgatg ctactggtaa agtgacattc aaaaatgacg
     2701 gtgatagcaa tacagtaata atcacagcgc ccccaagatc cggtggtgct atttaccaaa
     2761 cagttcctcc tgaatcgcgt tcggtgtgac tatgatcatt ttcggtgagt attgctttga
     2821 tccaaaggct ggttttctgt gatgattgac cagcctcaga gtattgatga caccaccgtt
     2881 cataacagat cggcattact atgcataagt actgcctcaa attctcgcca aaacactttt
     2941 taatgccttt gcgaattcat ccagacttat attgtcaatt ttttgcccaa cgacgactga
     3001 ctccgaaaca tcggccattg cgggccatcg cgttttgtaa tcagcataaa ttgcaacaac
     3061 aggggtatta taggcacttg ccatatgtaa tattgaggtg tcaggagtta ttgcaatata
     3121 agcatctttt atgattgctg ctgaacgttt aatcgagggt gataaggata aacggtagac
     3181 attattataa caatccacta atgccctggc tttatcttcc cctttgggtc cataaacgat
     3241 cactattgga atatctgttt cactttgaat ttttgctatt agattttctg caatcgataa
     3301 tgaaaatgta cgttcctgcg agctaccgtc taaattgagc gcaatgtaag atcctaacga
     3361 tcgcatttcc tcgcgtacct catcgagtac atcatcgctt aatggcaatt catatattga
     3421 cctgactgcc ggaaaacccg cctcacgcat cataaatgcc catgtcatgg gtacaggagt
     3481 ccggagattc tgatccatcc gggacgcgtt cttacacaaa ggggaatagc acttcatggt
     3541 taaaccgacg acctggaaat tcgtttttgc tttcaactga ctgataaata tcatcgtttt
     3601 caggttcttt ttacgcatcg cctctataca aagatccggc gtaccgtatt gcagtgttat
     3661 attcttcgct aaatctttta tttcttttaa tgttgcgtga
//
LOCUS       DAZKHF020000330         3697 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2201S31241
            SAMN43267800-rid25106413.denovo.332, whole genome shotgun sequence.
ACCESSION   DAZKHF020000330 DAZKHF020000000
VERSION     DAZKHF020000330.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267800
            Sequence Read Archive: SRR30307263
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3697)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3697)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 3697)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350581.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350581.2
            Genome Coverage       :: 151x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:21:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,652
            CDSs (total)                      :: 5,557
            Genes (coding)                    :: 5,320
            CDSs (with protein)               :: 5,320
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 237
            CDSs (without protein)            :: 237
            Pseudo Genes (ambiguous residues) :: 0 of 237
            Pseudo Genes (frameshifted)       :: 102 of 237
            Pseudo Genes (incomplete)         :: 130 of 237
            Pseudo Genes (internal stop)      :: 52 of 237
            Pseudo Genes (multiple problems)  :: 41 of 237
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3697
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267800-rid25106413.denovo.332"
                     /serotype="O 26:H11"
                     /isolate="2201S31241"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-01"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            complement(78..365)
                     /locus_tag="ACHOSC_RS22370"
                     /old_locus_tag="pgaptmp_004474"
     CDS             complement(78..365)
                     /locus_tag="ACHOSC_RS22370"
                     /old_locus_tag="pgaptmp_004474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008657 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) inhibitor activity
                     [Evidence IEA];
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA];
                     GO_process: GO:0030541 - plasmid partitioning [Evidence
                     IEA];
                     GO_process: GO:0051290 - protein heterotetramerization
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HGU4550271.1"
                     /translation="MLPILWLPSARDDLRQIITYIAKENPPAARRLKIRIETSVLPLS
                     EHPYLYPPSERVSGLREIVTHPNYIILYRVAASSIEIVSVTHSRRQFPFSI"
     gene            complement(365..556)
                     /locus_tag="ACHOSC_RS22375"
                     /old_locus_tag="pgaptmp_004475"
     CDS             complement(365..556)
                     /locus_tag="ACHOSC_RS22375"
                     /old_locus_tag="pgaptmp_004475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antitoxin"
                     /protein_id="HGU4550272.1"
                     /translation="MGTALSPIVSEFETTEQENSYNEWLRTKVTSSLADTRPAIPHDE
                     VMAEMENLIAQIAVTNKSE"
     gene            complement(584..985)
                     /locus_tag="ACHOSC_RS22380"
                     /old_locus_tag="pgaptmp_004476"
     CDS             complement(584..985)
                     /locus_tag="ACHOSC_RS22380"
                     /old_locus_tag="pgaptmp_004476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309096.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HGU4550273.1"
                     /translation="MMSALEVSMYRISKLLQETGWSQAELARRIGVTQQTVQQWVSGK
                     ATPKASSLDKLVEVSGHPLHWFLLPPEECEQIFTPDTMKIGPRQRELLQAFSAFPEED
                     QEKMLQEIKDKKKSMEETIARWLAAQKSRRA"
     gene            1094..1366
                     /locus_tag="ACHOSC_RS22385"
                     /old_locus_tag="pgaptmp_004477"
     CDS             1094..1366
                     /locus_tag="ACHOSC_RS22385"
                     /old_locus_tag="pgaptmp_004477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HGU4550274.1"
                     /translation="MLDSTREKIRQKYTQAEIGRYMGVAQQTVWQWFSFGVPPKQVIP
                     LCQLMKWEVTPHEIRPDIYPNPTDGLPVGCKVNTSNAPELIHENQA"
     gene            1350..1775
                     /locus_tag="ACHOSC_RS22390"
                     /old_locus_tag="pgaptmp_004478"
     CDS             1350..1775
                     /locus_tag="ACHOSC_RS22390"
                     /old_locus_tag="pgaptmp_004478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxin YdaT family protein"
                     /protein_id="HGU4550275.1"
                     /translation="MKIKHEHIRMAMNAWAHPDGEKVPAAKITKAYFELGMTFPELYD
                     DSHPEAMARNTQKIFRWVEKDTPDAVKKIQALLPAIEKAMPPPLVARMRSHSSAYFRE
                     LVETKERLVKDIDDFVASAIVLFDQMNRGGPAGNTLAVH"
     gene            complement(1982..2437)
                     /locus_tag="ACHOSC_RS22395"
                     /old_locus_tag="pgaptmp_004479"
     CDS             complement(1982..2437)
                     /locus_tag="ACHOSC_RS22395"
                     /old_locus_tag="pgaptmp_004479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4550276.1"
                     /translation="MYFLKSLYQAHVLNVAATNRWCNSPEMLPDYRAWLRAETYLRLD
                     ILISELQKETASIHNLQGIDAVRILVSRHSALSIIEVRHLSFSELIFLLQPALESANI
                     PPEVIQYPPHVDEQLQDVPYNQRAGLTPCSEAEWDHSLLKKYQDLYNPQ"
     gene            2516..3631
                     /locus_tag="ACHOSC_RS22400"
                     /old_locus_tag="pgaptmp_004480"
     CDS             2516..3631
                     /locus_tag="ACHOSC_RS22400"
                     /old_locus_tag="pgaptmp_004480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HGU4550277.1"
                     /translation="MRDYAKVSPRFWLGETGRELRKAGAEAQVVAFYLMTSPHANMLG
                     LYYLPVLYLAHETGLGLEGASKGLKRAVEAGFCSYDHDAEMVWVHEMAAWQVGETLKP
                     GDNRCAGVRNEYASLPENAFLSVFYDRYKTDFHLDVRRNNSRNSVRGFEGAFKGLRSQ
                     EQEQEQEKEQEQDKNTMVHGKKNTTNQAGDVQTVNPGQPAGTTPEADSAYALKADSGA
                     VQQVMTAGSEQSHQLQQPEADSAIQREADRVVPESTGQSVGRVDYPDVFEQVWREYPL
                     RAGANPKKSAFSAWKARLREGVPPETMLDGVRRYARYLAATGKAGTEFVQRATTFFGP
                     DRNFENPWLLPVSGTNNQRCVNHISEPDTEIPPGFRG"
BASE COUNT          974 a          786 c          997 g          940 t
ORIGIN      
        1 gttttgtgtc aacagttttt aattgttttt ttgggcaaaa aaatccctcg aaagagggag
       61 tatgaaaatt gttcaactca gatagagaag ggaaattgtc gccgagaatg tgtcacgctt
      121 acaatctcaa tgcttgaagc agctactctg tacaggatta tgtagttagg gtgggtcacg
      181 atctctctca atccagaaac ccgttcgctt ggtggatata agtacggatg ctcagataga
      241 ggtaataccg atgtttcaat gcgtattttt agtctacgtg ctgccggtgg gttctccttg
      301 gcgatgtaag ttatgatctg gcgcaaatca tcgcgagcag acggtagcca taaaatgggt
      361 aacattactc gctcttgtta gttacagcaa tttgagcaat aagattttcc atttcagcca
      421 ttacctcgtc atgtggaatt gcggggcgag tgtctgcaag gcttgacgtt actttagtgc
      481 gcaaccattc gttgtaactg ttttcttgtt cggtagtttc gaattctgaa actatcggag
      541 aaagggctgt acccatggca taactcctct tcttgtactg tggtcacgcc cggcggcttt
      601 tttgtgccgc cagccaccta gcaatggttt cttccattga ttttttcttg tctttgattt
      661 cttgaagcat tttttcttgg tcttcctcag gaaacgcact aaaagcttgg agcagttcgc
      721 gttggcgagg accaattttc atcgtgtcag gggtgaaaat ttgctcacac tcttcaggag
      781 gcaataaaaa ccaatgcaat ggatgccctg aaacctcaac cagtttatcc aaacttgagg
      841 ctttaggtgt agccttaccg ctgacccatt gttgaacagt ttgttgtgtc acaccaattc
      901 tacgggcaag ctcagcctgg ctccatccag tttcctgaag aagcttgctg attctgtaca
      961 tagatacttc tagggcgctc atcattattc aattttacag gtaaatactg ttaaaagcat
     1021 cacaataaaa aactgttgat tgcatacagt tttttattgt aggctttgct tatagttttt
     1081 agaggagggc aaaatgctag atagcactcg cgaaaaaatt aggcagaaat acactcaggc
     1141 tgaaataggt cgttatatgg gggtcgctca acagactgtt tggcaatggt ttagctttgg
     1201 cgttccccca aagcaggtaa ttccgttatg ccaactaatg aagtgggaag ttaccccgca
     1261 tgaaatccgc ccagatattt atcctaaccc aaccgacggt ttacctgttg gatgtaaggt
     1321 taacacatca aatgcgccgg agttgattca tgaaaatcaa gcatgaacac atccgcatgg
     1381 cgatgaatgc ctgggcgcat ccggacggcg aaaaagtacc ggctgcgaaa attaccaaag
     1441 cgtatttcga gctgggaatg acgttcccgg aactgtatga cgacagccat ccggaagcca
     1501 tggctcgcaa tactcagaaa attttccgct gggtggagaa agacactcct gatgcggtta
     1561 aaaaaattca ggcgttgtta ccagcgatcg aaaaagcgat gccgcctccg ctggtggccc
     1621 gaatgcgcag ccacagttcc gcttattttc gggagttggt agagacgaag gaacggctgg
     1681 tgaaagatat tgatgatttc gttgcatcag cgatcgttct gttcgatcag atgaatcgtg
     1741 gtggcccggc aggaaacact ctggctgtgc attaattggg taataaatat gagtaatgac
     1801 aaaaaattga cactgagcgt ttacgaaaac agtccgcaca tctggcgtgg cggtttatct
     1861 gatgtggagc tggcagagtg gttgatacat aaagctaatg cgctgctctg gcgtttgtca
     1921 gccagagaac agcgcaagga aaccagaata aagctggctg atgcagaagc gtgtgccggg
     1981 cttattgagg attatacaaa tcttggtatt tcttcagcag agagtgatcc cattcagcct
     2041 ctgagcaggg agtcaatcca gcacgctggt tgtatggcac atcttgtaac tgctcgtcaa
     2101 catgaggtgg gtattggatc acttccggtg ggatattcgc tgattccaga gctggttgaa
     2161 gcaagaaaat cagttcagaa aaagagagat gacgcacttc aattattgag agagcactat
     2221 ggcgcgatac cagaatgcga acagcatcga taccctgaag gttatgaatg gatgcagtct
     2281 ctttttgaag ttcgctaatc aatatgtcga gacgaaggta tgtttcggcg cgcagccagg
     2341 ctctgtaatc cgggagcatt tcggggctgt tacaccagcg gtttgttgct gcaacattta
     2401 atacatgagc ctgataaagg cttttcaaaa aatacatgtc gaacctcctc tggttctgtc
     2461 gattgggaac cacagattat atccggagga aggttcggca ccagatgagg tagccatgcg
     2521 tgattacgca aaagtttctc cgcgattctg gctgggagaa acggggagag aacttagaaa
     2581 ggcgggtgca gaagcgcaag ttgttgcttt ttacctgatg acatcccctc acgcaaatat
     2641 gctgggtttg tattacctgc cagttttata ccttgctcat gaaaccgggc ttggtctgga
     2701 aggggcttca aaggggctta aaagggctgt tgaagctggt ttttgtagct atgaccatga
     2761 tgcagagatg gtctgggtcc atgaaatggc agcctggcag gttggggaaa cgttgaagcc
     2821 tggcgataac cgttgtgcag gtgtcaggaa tgagtatgca tcattacctg aaaacgcttt
     2881 tctgtcagtg ttttacgaca gatataaaac ggatttccat ctggatgtga ggcggaataa
     2941 tagccgaaat tcggtaaggg gcttcgaagg ggcttttaag gggcttcgaa gccaagaaca
     3001 ggaacaggag caggagaaag aacaggaaca ggacaaaaac actatggttc atggcaaaaa
     3061 aaacaccacg aaccaggcag gggatgttca gaccgtcaat cctggtcagc cagcaggcac
     3121 gacaccggaa gccgattcag cgtatgcgct gaaagccgat tcgggcgctg tgcagcaggt
     3181 gatgaccgca gggtcggagc aatcacacca actgcagcag cctgaagccg attccgccat
     3241 tcagcgggaa gccgatcggg tagtcccgga aagcaccggg cagtctgtgg gacgagtgga
     3301 ttatccggat gtgttcgaac aggtctggcg ggaatacccg ttgcgtgctg gggcaaaccc
     3361 gaagaaatcc gctttcagtg cctggaaggc cagattgcgc gagggggtgc caccagagac
     3421 catgctggat ggtgtgaggc gttacgcgag atacctggcg gcgaccggga aagcgggaac
     3481 ggaatttgtt cagcgagcga cgacgttttt tgggccggac cggaattttg aaaacccctg
     3541 gttgctcccg gtaagcggca cgaacaacca gcgttgtgtg aatcatattt ctgaaccgga
     3601 taccgaaatt ccgccgggat tcagggggtg atgtggcatg aaaaacattg cggcagccgg
     3661 ggttcttgaa cgtattcgca gacttgcacc acaggcg
//
LOCUS       DAZKHF020000331         3662 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2201S31241
            SAMN43267800-rid25106413.denovo.333, whole genome shotgun sequence.
ACCESSION   DAZKHF020000331 DAZKHF020000000
VERSION     DAZKHF020000331.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267800
            Sequence Read Archive: SRR30307263
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3662)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3662)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 3662)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350581.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350581.2
            Genome Coverage       :: 151x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:21:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,652
            CDSs (total)                      :: 5,557
            Genes (coding)                    :: 5,320
            CDSs (with protein)               :: 5,320
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 237
            CDSs (without protein)            :: 237
            Pseudo Genes (ambiguous residues) :: 0 of 237
            Pseudo Genes (frameshifted)       :: 102 of 237
            Pseudo Genes (incomplete)         :: 130 of 237
            Pseudo Genes (internal stop)      :: 52 of 237
            Pseudo Genes (multiple problems)  :: 41 of 237
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3662
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267800-rid25106413.denovo.333"
                     /serotype="O 26:H11"
                     /isolate="2201S31241"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-01"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            complement(<1..167)
                     /locus_tag="ACHOSC_RS22405"
                     /old_locus_tag="pgaptmp_004481"
     CDS             complement(<1..167)
                     /locus_tag="ACHOSC_RS22405"
                     /old_locus_tag="pgaptmp_004481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4550278.1"
                     /translation="MNEIKEIPVVRDEYGCWTHPEYEKFCDGREYISTEEFNAWMEEN
                     NLQYVLCFRDEG"
     gene            complement(160..414)
                     /locus_tag="ACHOSC_RS22410"
                     /old_locus_tag="pgaptmp_004482"
     CDS             complement(160..414)
                     /locus_tag="ACHOSC_RS22410"
                     /old_locus_tag="pgaptmp_004482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001294161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4550279.1"
                     /translation="MSESKCQVNGNKIEPCAALAKSLEHDAEYTMRKGLLIYKIWNES
                     LTRGPDFVMLRSGEFSKLPVRVSFCPFCGESLKTWENRNE"
     gene            complement(411..833)
                     /locus_tag="ACHOSC_RS22415"
                     /old_locus_tag="pgaptmp_004483"
     CDS             complement(411..833)
                     /locus_tag="ACHOSC_RS22415"
                     /old_locus_tag="pgaptmp_004483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001151202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF977 family protein"
                     /protein_id="HGU4550280.1"
                     /translation="MAKVFTQEEREKIKGQVVELVRQSGRETLRQLETKTGATRYLMS
                     VLARELVASGDVYNSGYGLFPSEQARKDWQNARKKLSRAKPKKPSAVDPDLIWSLPDG
                     EIRRYDRRLNIICLECRKSEVMQRVLAFYQGNFEEVVR"
     gene            complement(874..1836)
                     /locus_tag="ACHOSC_RS22420"
                     /old_locus_tag="pgaptmp_004484"
     CDS             complement(874..1836)
                     /locus_tag="ACHOSC_RS22420"
                     /old_locus_tag="pgaptmp_004484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310303.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HGU4550281.1"
                     /translation="MSSKLHGLVWEGCAFTGMILSRVAVMARLADYSNDEGVSWPAIE
                     TIRRQIGARSESTVKSAIAELAKEGWLTKEERKVGGRNVSNIYRLNVEKLEAAAAAAR
                     ESYKPKRKISPAKNDPLTVDPSNIDPSTVDPSNFDGSTVDKKLPIRGPMIDPDPSVLK
                     PDPSDKRSSCPDASQPDPQTAEQDFLTRHPDAVVFSAKKRQWGSQEDLVCAQWIWGRI
                     VSLYEQAASYDGEITRPKEPNWTAWANDVRTMRMLDGRTHRQICEMFGRLQRDSFWVK
                     NIMSPAKLREKWDELVIRLGRSPAQRCVNHISEPDTEIPPGFRG"
     gene            complement(1859..2284)
                     /locus_tag="ACHOSC_RS22425"
                     /old_locus_tag="pgaptmp_004485"
     CDS             complement(1859..2284)
                     /locus_tag="ACHOSC_RS22425"
                     /old_locus_tag="pgaptmp_004485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxin YdaT family protein"
                     /protein_id="HGU4550282.1"
                     /translation="MKIKHEHIRMAMNVWAHPDGEKVPAAKITKAYFELGMTFPELYD
                     DSHPEALARNTQKIFRWLDKDTPDAVEKMQALLPAIEKAMPPLLVARMRSHSSEYYRE
                     IVERRDRLVKDVDDFVASAVVLYDQMNRGGPAGNAVVMH"
     gene            complement(2281..2583)
                     /locus_tag="ACHOSC_RS22430"
                     /old_locus_tag="pgaptmp_004486"
     CDS             complement(2281..2583)
                     /locus_tag="ACHOSC_RS22430"
                     /old_locus_tag="pgaptmp_004486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HGU4550283.1"
                     /translation="MTLKEFIKSLRVGDAKKFAARLGVSPSYLSQMASGRIAISPTRA
                     LMIESATEGQVSRAELRPHDWELIWPEYASGIRLGQTHVVHAEGDCSACLSDGVDS"
     gene            2681..3052
                     /locus_tag="ACHOSC_RS22435"
                     /old_locus_tag="pgaptmp_004487"
     CDS             2681..3052
                     /locus_tag="ACHOSC_RS22435"
                     /old_locus_tag="pgaptmp_004487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310306.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4550284.1"
                     /translation="MQKKEIRRLRLKEWFKDKTLPPKEKSYLSQLMSGRASFGEKAAR
                     RIEQTYGMPEGYLDAEYAEQPGVSPPHAGLTSNQLELLQIFSAFPEDEQRQIISELKQ
                     KKESMEDLIARWIAAQKCRRA"
     gene            3073..3264
                     /locus_tag="ACHOSC_RS22440"
                     /old_locus_tag="pgaptmp_004488"
     CDS             3073..3264
                     /locus_tag="ACHOSC_RS22440"
                     /old_locus_tag="pgaptmp_004488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310307.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stability determinant"
                     /protein_id="HGU4550285.1"
                     /translation="MNRALSPMVSEFETIEQENSYNEWLRAKVATSLADPRPAIPHDE
                     VERRMAERFAKMRKERSKQ"
     gene            3266..3544
                     /locus_tag="ACHOSC_RS22445"
                     /old_locus_tag="pgaptmp_004489"
     CDS             3266..3544
                     /locus_tag="ACHOSC_RS22445"
                     /old_locus_tag="pgaptmp_004489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008657 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) inhibitor activity
                     [Evidence IEA];
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA];
                     GO_process: GO:0030541 - plasmid partitioning [Evidence
                     IEA];
                     GO_process: GO:0051290 - protein heterotetramerization
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HGU4550286.1"
                     /translation="MLPVLWLESADTDLDDITSYIARFDIDAAERLWQRLRGCVLPLS
                     EHPYLYPPSDRVPGLREIVAHPNYIILYRVTTSSVEVVNVIHARRQFP"
BASE COUNT          947 a          963 c          777 g          975 t
ORIGIN      
        1 ccttcatctc tgaagcagag gacgtattga agattatttt cctccatcca ggcgttaaac
       61 tcttccgttg aaatatattc ccgaccgtca cagaattttt catattcagg atgcgtccag
      121 cagccatatt catcacgtac tactggtatt tctttaattt cattcatttc tgttctccca
      181 cgttttcaga ctttcaccac agaacggaca aaatgaaacc cgaactggta atttagaaaa
      241 ttcaccggaa cgcaacatca caaaatcagg accgcgagtt aaactctcat tccagatttt
      301 gtatatcagc agaccttttc gcatcgtgta ttcagcatca tgctcaaggg actttgccag
      361 tgctgcacat ggttctatct tgttgccatt aacctggcat tttgattcac tcaccgcacc
      421 acctcctcaa aattcccctg ataaaacgcc agtacgcgct gcataacttc gctcttccgg
      481 cactcgagac agattatgtt cagacgcctg tcgtagcggc gtatttcgcc atctggtaat
      541 gaccagataa ggtccggatc aaccgcagat ggtttcttcg gctttgccct tgagagcttt
      601 ttacgggcat tttgccagtc cttacgcgcc tgttcagacg ggaataaccc gtaaccagag
      661 ttgtatacat cgccactggc aaccagctct ctggccagaa cgctcatcag atatcttgtt
      721 gccccagttt tagtttccag ttgtcgtaac gtctcgcgcc cactctggcg tacgagttca
      781 acaacctgcc ctttaatttt ttcccgctct tcttgtgtaa aaacttttgc cacaagccct
      841 cctgaaaatt acctcatgac cagaaattaa cacttacccc ctgaagcccg gcggaatttc
      901 agtgtccggt tcagaaatgt gattcacgca acgctgcgca ggcgaacgcc ccaggcggat
      961 aaccagttca tcccattttt cccggagttt tgccggactc atgatgtttt ttacccagaa
     1021 cgaatcccgc tggagacgcc caaacatttc acaaatttgt ctgtgagttc tgccatccag
     1081 catccgcatt gtgcgaacgt cattggccca tgctgtccag ttgggttctt tcggtctagt
     1141 gatctcgcca tcatagctgg ccgcctgctc gtaaagactc acgattcgtc cccagatcca
     1201 ctgtgcgcac accaaatctt cctgacttcc ccactggcgt tttttcgcac tgaacacaac
     1261 cgcgtcaggg tgtcgggtta aaaaatcctg ttcagccgtc tgcgggtccg gttgcgaagc
     1321 gtccggacaa gaagatcttt tatctgacgg atcaggtttt aatactgacg gatcggggtc
     1381 aatcatcggc cccctaatcg gcagtttttt atcaacagtt gatccatcaa aatttgacgg
     1441 gtcaaccgtt gaggggtcaa tatttgacgg gtcaactgtt aacgggtcat tttttgccgg
     1501 gctaattttt cttttcggtt tatatgactc acgcgccgcc gccgcagctg cttcgagttt
     1561 ttccacatta agccgataga tattgcttac attacgccca ccgaccttac gctcttcctt
     1621 cgtcagccag ccctctttcg ccagttctgc aatagccgat ttcactgtgg attcacttct
     1681 tgcaccgatc tgacgccgga tagtttcaat ggcaggccat gacacgccct cgtcattgct
     1741 gtagtctgca agacgggcca taaccgccac cctggataag atcatgccgg tgaaggcgca
     1801 cccttcccag acaagaccat gaagcttgct gctcataaaa cccccgaaca ccgtgctttt
     1861 agtgcatcac cacagcattc cctgccgggc cgccgcgatt catctggtca tacaaaacaa
     1921 ccgctgacgc aacaaaatca tcgacatcct tcaccagccg atccctccgt tcgacgatct
     1981 cacggtaata ttcagaactg tggctgcgca tacgggccac cagcaaaggc ggcatcgcct
     2041 tttcgatcgc cggtaacaga gcctgcattt tttcaacagc atcaggggtg tctttatcca
     2101 gccaacggaa aattttctgg gtattacggg ccagggcttc cggatggctg tcgtcataca
     2161 gttccgggaa cgtcattccc agctcgaaat acgctttggt aattttcgca gccggtactt
     2221 tttcgccgtc cggatgcgcc cagacattca tcgccatgcg gatgtgttca tgcttgattt
     2281 tcatgaatca actccatcag ataagcatgc actacaatca ccttcagcat gaactacatg
     2341 tgtttgcccc aaacgaatgc cgctcgcata ctcaggccaa ataagctccc aatcatgagg
     2401 tcgtagctcc gccctactta cttggccttc cgtcgcagat tcgatcataa gggcgcgggt
     2461 tggagatata gctattcgtc cagacgccat ttgcgataag taagatggcg atacaccaag
     2521 tctggccgcg aatttcttag catcaccaac ccttaatgat ttaataaact cttttaatgt
     2581 cataccttcc tcggtttagt gtttttttgc gagtttagtg tttaataaac cattaagtca
     2641 agtatttgct tgtttagtga ttactaaaga taattaccac atgcaaaaaa aagaaattcg
     2701 ccgtttacgt ctcaaggagt ggtttaaaga taaaactctg ccacccaaag agaagagcta
     2761 cctatctcaa ctaatgagtg ggagagcctc gtttggagaa aaggctgcca gaagaataga
     2821 gcaaacatac gggatgccgg aagggtatct ggatgcggaa tacgcagaac aaccgggggt
     2881 ttctccacca catgcagggt taacgtctaa tcaactggaa ttattgcaga ttttttcagc
     2941 cttccctgag gatgagcaac gccagataat cagcgagtta aagcagaaaa aagaatcaat
     3001 ggaagatctc atagcgagat ggattgcggc gcaaaaatgc cgccgcgcct gagttataaa
     3061 accggaggaa acatgaatag agccctttca ccaatggttt ctgaatttga aaccattgaa
     3121 caagaaaaca gttacaacga atggctgcgt gcgaaagtag caacgagcct tgcagatccg
     3181 cgcccagcaa ttccccatga cgaagttgag cgcagaatgg cagaacgctt tgctaagatg
     3241 cgcaaggaac ggagcaagca gtaaaatgtt acccgtgtta tggcttgaaa gcgcagatac
     3301 cgacctagat gatataacta gttatattgc tcgtttcgac atagatgcgg ctgaacgctt
     3361 atggcagcga ttaaggggtt gtgtgctgcc gttatccgaa catccgtatt tatacccacc
     3421 aagcgacaga gtacctggct tgcgtgagat tgtagcccac cctaactata taattctata
     3481 ccgcgtaaca acatcaagcg ttgaagtagt aaacgtgatc cacgcaagac gccagtttcc
     3541 ctaactttca ctaccaatag aaacataaca accgcaacga ctttatcaaa agcgttgtgt
     3601 ttgttatgtc ccgcggttta gtttttactt gacttaagtt taatgtttat taaactaaaa
     3661 at
//
LOCUS       DAZKHF020000332         3623 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2201S31241
            SAMN43267800-rid25106413.denovo.334, whole genome shotgun sequence.
ACCESSION   DAZKHF020000332 DAZKHF020000000
VERSION     DAZKHF020000332.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267800
            Sequence Read Archive: SRR30307263
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3623)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3623)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 3623)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350581.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350581.2
            Genome Coverage       :: 151x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:21:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,652
            CDSs (total)                      :: 5,557
            Genes (coding)                    :: 5,320
            CDSs (with protein)               :: 5,320
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 237
            CDSs (without protein)            :: 237
            Pseudo Genes (ambiguous residues) :: 0 of 237
            Pseudo Genes (frameshifted)       :: 102 of 237
            Pseudo Genes (incomplete)         :: 130 of 237
            Pseudo Genes (internal stop)      :: 52 of 237
            Pseudo Genes (multiple problems)  :: 41 of 237
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3623
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267800-rid25106413.denovo.334"
                     /serotype="O 26:H11"
                     /isolate="2201S31241"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-01"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            129..1232
                     /gene="proB"
                     /locus_tag="ACHOSC_RS22450"
                     /old_locus_tag="pgaptmp_004490"
     CDS             129..1232
                     /gene="proB"
                     /locus_tag="ACHOSC_RS22450"
                     /old_locus_tag="pgaptmp_004490"
                     /EC_number="2.7.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004349 - glutamate 5-kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate 5-kinase"
                     /protein_id="HGU4550287.1"
                     /translation="MSDSQTLVVKLGTSVLTGGSRRLNRAHIVELVRQCAQLHAAGHR
                     IVIVTSGAIAAGREHLGYPELPATIASKQLLAAVGQSRLIQLWEQLFSIYGIHVGQML
                     LTRADMEDRERFLNARDTLRALLDNNIVPVINENDAVATAEIKVGDNDNLSALAAILA
                     GADKLLLLTDQKGLYTADPRSNPQAELIKDVYGIDDALRAIAGDSVSGLGTGGMSTKL
                     QAADVACRAGIDTIIAAGSKPGVIGDVMEGISVGTLFHAQATPLENRKRWIFGAPPAG
                     EITVDEGATAAILERGSSLLPKGIKSVTGNFSRGEVIRICNLEGRDIAHGVSRYNSDA
                     LRRIAGHHSQEIDAILGYEYGPVAVHRDDMITR"
     gene            1244..2497
                     /gene="proA"
                     /locus_tag="ACHOSC_RS22455"
                     /old_locus_tag="pgaptmp_004491"
     CDS             1244..2497
                     /gene="proA"
                     /locus_tag="ACHOSC_RS22455"
                     /old_locus_tag="pgaptmp_004491"
                     /EC_number="1.2.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004350 - glutamate-5-semialdehyde
                     dehydrogenase activity [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-5-semialdehyde dehydrogenase"
                     /protein_id="HGU4550288.1"
                     /translation="MLEQMGIAAKQASYKLAQLSSREKNRVLEKIADELEAQSEIILN
                     ANAQDVADARANGLGEAMLDRLALTPARLKGIADDVRQVCNLADPVGQVIDGSVLDSG
                     LRLERRRVPLGVIGVIYEARPNVTVDVASLCLKTGNAVILRGGKETCRTNAATVAVIQ
                     DALKSCGLPAGAVQAIDNPDRALVSEMLRMDKYIDMLIPRGGAGLHKLCREQSTIPVI
                     TGGIGVCHIYVDESVEIAEALKVIVNAKTQRPSTCNTVETLLVNKNIADSFLPALSKQ
                     MAESGVTLHADAAALAQLQAGPAKVVAVKAEEYDDEFLSLDLNVKIVSDLDDAIAHIR
                     EHGTQHSDAILTRDMRNAQRFVNEVDSSAVYVNASTRFTDGGQFGLGAEVAVSTQKLH
                     ARGPMGLEALTTYKWIGIGDYTIRA"
     gene            2612..2687
                     /locus_tag="ACHOSC_RS22460"
                     /old_locus_tag="pgaptmp_004492"
     tRNA            2612..2687
                     /locus_tag="ACHOSC_RS22460"
                     /old_locus_tag="pgaptmp_004492"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2645..2647,aa:Thr,seq:cgt)
     gene            3292..3450
                     /locus_tag="ACHOSC_RS22465"
                     /old_locus_tag="pgaptmp_004493"
     CDS             3292..3450
                     /locus_tag="ACHOSC_RS22465"
                     /old_locus_tag="pgaptmp_004493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001570982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4550289.1"
                     /translation="MKNIAAIKRNNRKIHARKFLSTPEGKAWLERKQRENEERKLLSE
                     LKWLREDF"
BASE COUNT          906 a          867 c          979 g          871 t
ORIGIN      
        1 gccgctaggc ttgctgatcc cgcgcaacaa aacgccatgc tttgctcgca gatggttggc
       61 aaccgacgac agtcctgcta aaacgttcgt ttgatatcat ttttcctaaa attgaatggc
      121 agagaatcat gagtgacagc cagacgctgg tggtaaaact cggcaccagt gtgctaacag
      181 gcggatcgcg ccgtctgaac cgtgcccata tcgttgaact tgttcgccag tgcgcgcagt
      241 tacatgccgc cgggcatcgg attgttattg tgacgtcggg cgcgatcgcc gccggacgtg
      301 agcacctggg ttacccggaa ctgccagcga ctatcgcctc gaaacaactg ctggcagcgg
      361 tagggcagag tcgactgatt caactgtggg aacagctgtt ttcgatttat ggcattcacg
      421 tcgggcaaat gctgctgacc cgtgctgata tggaagaccg tgaacgcttc ctgaacgccc
      481 gcgacaccct gcgtgcgttg ctcgataaca atatcgttcc ggtaatcaat gagaacgatg
      541 ctgtcgctac ggcagagatt aaagtcggcg ataacgataa cctttctgcg ctggcggcga
      601 ttctggcggg tgccgataaa ctgttgctgc tgaccgatca aaaaggtttg tataccgctg
      661 acccgcgcag caatccgcag gcagaactga ttaaagatgt ttacggcatt gatgacgcac
      721 tgcgcgcgat tgccggtgac agcgtttcag gcctcggaac tggcggcatg agtaccaaat
      781 tgcaggctgc tgacgtggct tgccgtgcgg gtatcgacac cattattgcc gcgggcagca
      841 agccgggcgt tattggtgat gtgatggaag gcatttccgt cggtacgctg ttccatgccc
      901 aggcgactcc gcttgaaaac cgtaaacgct ggattttcgg tgcgccgccg gcgggtgaaa
      961 tcacggtaga tgaaggggca actgccgcca ttcttgaacg cggcagctcc ctgttgccga
     1021 aaggcattaa aagcgtgact ggcaatttct cgcgtggtga agtcatccgc atttgcaacc
     1081 tcgaaggtcg cgatatcgcc cacggcgtca gtcgttacaa cagcgatgca ttacgccgta
     1141 ttgccgggca ccactcgcaa gaaattgatg caatactggg atatgaatac ggcccggttg
     1201 ccgttcaccg tgatgacatg attacccgtt aaggagcagg ctgatgctgg aacaaatggg
     1261 cattgccgcg aagcaagcct cgtataaatt agcgcaactc tccagccgcg aaaaaaatcg
     1321 cgtgctggaa aaaatcgccg atgaactgga agcacaaagc gaaatcatcc tcaacgctaa
     1381 cgcccaggat gttgctgacg cgcgtgccaa tggccttggc gaagcgatgc ttgaccgtct
     1441 ggcactgacg cccgcacggc tgaaaggcat tgccgatgat gtgcgccagg tgtgtaacct
     1501 cgccgatccg gtggggcagg taatcgatgg cagcgtactg gacagcggcc tgcgtcttga
     1561 gcgtcgtcgc gtaccgctgg gggttattgg cgtgatttat gaagcgcgcc cgaacgtgac
     1621 ggttgatgtc gcttcgctgt gcctgaaaac cggtaatgcg gtgatcctgc gcggtggcaa
     1681 agaaacgtgt cgcactaacg ctgcaacggt ggcggtgatt caggacgccc tgaaatcctg
     1741 cggcttaccg gcgggtgccg tgcaggcgat tgataatcct gaccgtgcgc tggtcagtga
     1801 aatgctgcgt atggataaat acatcgacat gctgatcccg cgtggtggcg ctggtttgca
     1861 taaactgtgc cgtgaacagt cgacaatccc ggtgatcaca ggtggtatag gcgtatgcca
     1921 tatttacgtt gatgaaagtg tagagatcgc tgaagcatta aaagtgatcg tcaacgcgaa
     1981 aactcagcgt ccgagcacat gtaatacggt tgaaacgttg ctggtgaata aaaacatcgc
     2041 cgatagcttc ctgcccgcat taagcaaaca aatggcggaa agcggcgtga cattacacgc
     2101 agatgcagct gcactggcgc agttgcaggc aggccctgcg aaggtggttg ctgttaaagc
     2161 cgaagagtat gacgatgagt ttctgtcatt agatttgaac gtcaaaatcg tcagcgatct
     2221 tgacgatgcc atcgcccata ttcgtgaaca cggcacacaa cactccgatg cgatcctgac
     2281 ccgcgatatg cgcaacgccc agcgttttgt taacgaagtg gattcgtccg ctgtttacgt
     2341 taacgcctct acgcgtttta ccgacggcgg ccagtttggt ctgggtgcgg aagtggcggt
     2401 aagcacacaa aaactccacg cgcgtggccc aatggggctg gaagcactga ccacttacaa
     2461 gtggatcggc attggtgatt acaccattcg tgcgtaaata aaaccgggtg atgcaaaagt
     2521 agccatttga ttcacaaggc cattgacgca tcgcccggtt agttttaacc ttgtccaccg
     2581 tgattcacgt tcgtgaacat gtcctttcag ggccgatata gctcagttgg tagagcagcg
     2641 cattcgtaat gcgaaggtcg taggttcgac tcctattatc ggcaccatta agaaaatcaa
     2701 taacttagct taacttctct aagaaattgt atcttctgtt atcacttaca aacgcctata
     2761 cgttgttatg ggctttggta gtgtaacacg tatgggcgag tagttcccag gctcaacctc
     2821 gtaacaagtt cctttgaagc tggaagacgt gccaccgtct ctaaaccttc taccaaaact
     2881 cttagcaaga tgttccacac ctggcgcggt gtccaatgat gctacacgtc gtgagacgtt
     2941 gcagaggata aacaggctaa atgatgggaa gagttttaag tgcatagtgg attaaagccc
     3001 acctcttcaa aacctaactt gatcgggaaa gaatcaagct ctagcacggt ttcgtccacc
     3061 ggagtacaac gggtgcctga taagagaaga actgctaaga cttcatagct tttgtgaaaa
     3121 agcccttttt agtggatacg cctacggcgc ttattttttg gtataaccat caaaggcgct
     3181 ttatggctcc tattgtcttg aaaatgtatc tttacttgta ttgtgtttta tgcgtagtct
     3241 ccctgtgtag tatgtaactt gttaattttc atatgaatac agaggcacac gatgaaaaac
     3301 attgcagcta tcaaacgtaa taaccgcaag attcacgctc gtaagttcct gtctacgcca
     3361 gagggaaaag cctggctaga gcgaaagcaa cgagagaacg aagaaagaaa actccttagt
     3421 gagttgaaat ggcttagaga ggatttctga aaatctccag aacgcaacag gacgcattct
     3481 aacgctgttt tcattgcttg gcatagggat gtataggtga tgtgtgttta atgcgtatgt
     3541 gagcttgttt tgtgccttat tttgcatttt cataatgcag aagagaacac ggaaaacctt
     3601 aaaattaatt attggggtat tta
//
LOCUS       DAZKHF020000333         3623 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2201S31241
            SAMN43267800-rid25106413.denovo.335, whole genome shotgun sequence.
ACCESSION   DAZKHF020000333 DAZKHF020000000
VERSION     DAZKHF020000333.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267800
            Sequence Read Archive: SRR30307263
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3623)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3623)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 3623)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350581.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350581.2
            Genome Coverage       :: 151x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:21:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,652
            CDSs (total)                      :: 5,557
            Genes (coding)                    :: 5,320
            CDSs (with protein)               :: 5,320
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 237
            CDSs (without protein)            :: 237
            Pseudo Genes (ambiguous residues) :: 0 of 237
            Pseudo Genes (frameshifted)       :: 102 of 237
            Pseudo Genes (incomplete)         :: 130 of 237
            Pseudo Genes (internal stop)      :: 52 of 237
            Pseudo Genes (multiple problems)  :: 41 of 237
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3623
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267800-rid25106413.denovo.335"
                     /serotype="O 26:H11"
                     /isolate="2201S31241"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-01"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            complement(35..754)
                     /gene="dcuR"
                     /locus_tag="ACHOSC_RS22470"
                     /old_locus_tag="pgaptmp_004494"
     CDS             complement(35..754)
                     /gene="dcuR"
                     /locus_tag="ACHOSC_RS22470"
                     /old_locus_tag="pgaptmp_004494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator DcuR"
                     /protein_id="HGU4550290.1"
                     /translation="MINVLIIDDDAMVAELNRRYVAQIPGFQCCGTASTLEKAKEIIF
                     NSDTPIDLILLDIYMQKENGLDLLPVLHNARCKSDVIVISSAADAATIKDSLHYGVVD
                     YLIKPFQASRFEEALTGWRQKKMALEKHQYYDQAELDQLIHGSSSNEQDPRRLPKGLT
                     PQTLRTLCQWIDAHQDYEFSTDELANEVNISRVSCRKYLIWLVNCHILFTSIHYGVTG
                     RPVYRYRIQAEHYSLLKQYCQ"
     gene            complement(751..2382)
                     /locus_tag="ACHOSC_RS22475"
                     /old_locus_tag="pgaptmp_004495"
     CDS             complement(751..2382)
                     /locus_tag="ACHOSC_RS22475"
                     /old_locus_tag="pgaptmp_004495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HGU4550291.1"
                     /translation="MRHSLPYRMLRKRPMKLSTTVILMVSAVLFSVLLVVHLIYFSQI
                     SDMTRDGLANKALAVARTLADSPEIRQGLQKKPQESGIQAIAEAVRKRNDLLFIVVTD
                     MQSLRYSHPEAQRIGQPFKGDDILKALNGKENVAINRGFLAQALRVFTPIYDENHKQI
                     GVVAIGLELSRVTQQINDSRWSIIWSVLFGMLVGLIGTCILVKVLKKILFGLEPYEIS
                     TLFEQRQAMLQSIKEGVVAVDDRGEVTLINDAAQELLNYRKSQDDEKLSTLSHSWSQV
                     VDVSEVLRDGTPRRDEEITIKDRLLLINTVPVRSNGVIIGAISTFRDKTEVRKLMQRL
                     DGLVNYADALRERSHEFMNKLHVILGLLHLKSYKQLEDYILKTANNYQEEIGSLLGKI
                     KSPVIAGFLISKINRATDLGHTLILNSESQLPDSGSEDQVATLITTLGNLIENALEAL
                     GPEPGGEISVTLHYRHGWLHCEVNDDGPGIAPDKIDHIFDKGVSTKGSERGVGLALVK
                     QQVENLGGSIAVESEPGIFTQFFVQIPWDGERSNR"
     gene            2563..2793
                     /gene="yjdI"
                     /locus_tag="ACHOSC_RS22480"
                     /old_locus_tag="pgaptmp_004496"
     CDS             2563..2793
                     /gene="yjdI"
                     /locus_tag="ACHOSC_RS22480"
                     /old_locus_tag="pgaptmp_004496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S mono-cluster protein YjdI"
                     /protein_id="HGU4550292.1"
                     /translation="MDQALLDEGYRCYTGEKIDVYFNTAICQHSGNCVRGNGKLFNLK
                     RKPWIMPDEVDVVTVVKVIDTCPSGALKYRHK"
     gene            2805..3077
                     /locus_tag="ACHOSC_RS22485"
                     /old_locus_tag="pgaptmp_004497"
     CDS             2805..3077
                     /locus_tag="ACHOSC_RS22485"
                     /old_locus_tag="pgaptmp_004497"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HGU4550293.1"
                     /translation="MEIREGHNKFYINDEQGKQIAEIVFVPTGENLAIIEHTDVDESL
                     KGQGIGKQLVAKVVEKMRREKRKIIPLCPFAKHEFDKTREYDDIRS"
     gene            3304..3600
                     /gene="ghoS"
                     /locus_tag="ACHOSC_RS22490"
                     /old_locus_tag="pgaptmp_004498"
     CDS             3304..3600
                     /gene="ghoS"
                     /locus_tag="ACHOSC_RS22490"
                     /old_locus_tag="pgaptmp_004498"
                     /EC_number="3.1.26.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type V toxin-antitoxin system endoribonuclease
                     antitoxin GhoS"
                     /protein_id="HGU4550294.1"
                     /translation="MEGKNKFNTYVVSFDYPSSYSSVFLRLRSLMYDMNFSSIVADEY
                     GIPRQLNENSFAITTSLAASEIEDLIRLKCLDLPDIDFDLNIMTVDDYFRQFYK"
BASE COUNT          947 a          821 c          885 g          970 t
ORIGIN      
        1 taataattat tacttacaga taaccagcga tccgttattg gcaatattgt ttcagtagtg
       61 agtagtgttc tgcctgaata cggtaacggt aaaccggacg ccccgtgacg ccataatgga
      121 tactggtgaa caagatgtgg cagttgacca gccagatgag gtatttacgg caggaaacac
      181 gcgagatgtt aacctcgtta gccagctcgt cggttgaaaa ctcatagtcc tgatgcgcgt
      241 caatccactg gcacagcgtg cgtaaagtct gcggcgttaa gccttttggc aagcgacgag
      301 gatcctgttc gttggagctg ctgccgtgga ttagctgatc aagctcggcc tggtcataat
      361 actgatgttt ttccagcgcc attttctttt gccgccagcc ggtgagcgcc tcttcaaagc
      421 gggaagcctg gaagggtttg atcaggtaat ccacgacacc gtaatgcagc gaatctttaa
      481 tggttgccgc atcggctgcg gaggagatga caatcacatc acttttgcaa cgcgcgttat
      541 gcaggacagg cagtaaatcg agcccgttct ctttttgcat atagatatcg agcaatatca
      601 ggtcgatagg cgtatcgcta ttgaagataa tctctttggc tttctccagc gtcgaggctg
      661 ttccacagca ttgaaagcct gggatttgtg ctacgtatcg gcgattcagc tccgcgacca
      721 ttgcgtcgtc atcgataatt aatacattga tcatctgttc gacctctccc cgtcccaggg
      781 tatctggaca aaaaattgtg tgaaaatccc gggttccgat tccacggcga tgctgccgcc
      841 gagattttct acctgttgtt tgacaagtgc taaaccgacg cctcgctcgc ttccttttgt
      901 cgagacacct ttgtcaaaaa tgtgatcgat tttgtcgggt gcgattcccg gtccatcatc
      961 gttaacttcg cagtgcagcc agccgtgacg gtagtgcaat gttacgctaa tttcgccacc
     1021 gggttccggc cctaatgctt ccagcgcgtt ttctatcaga tttcccaatg tggtaatcag
     1081 cgtcgcgacc tggtcctcac tgccgctgtc tggcagctgg ctttcactgt ttaaaatcag
     1141 cgtatggcct aaatcggtcg cgcggttaat cttgctgatt aaaaaaccag cgataaccgg
     1201 agatttgatc ttacccagca gagagccaat ctcttcctga tagttattgg ctgttttgag
     1261 aatgtaatct tccaactgct tataactctt cagatgcaat aatccgagaa tcacatgcaa
     1321 tttattcata aattcgtggg atcgttcacg aagtgcatca gcatagttga ccagaccgtc
     1381 gagtcgctgc atcagtttac gtacttcagt tttgtccctg aaggttgaaa tggcaccgat
     1441 gataacgcca ttactgcgca ccggaacggt gttgatcagt aatagccggt ctttaatcgt
     1501 aatctcttcg tcgcggcgcg gcgtaccgtc gcgtaacact tccgagacat ctaccacctg
     1561 tgaccatgag tggctgagcg tcgacagttt ctcatcgtcc tgcgacttac ggtaattcag
     1621 caattcttgt gcggcatcgt tgatcagcgt gacctcgccg cgatcgtcca cggcaacgac
     1681 gccttctttg atagactgca acatggcctg gcgctgctca aacagcgtgg agatttcgta
     1741 gggttccagg ccgaaaagga tttttttcag taccttaacc agaatgcagg tgccaatcag
     1801 tccgaccagc atgccaaata ataccgacca gataatgctc cagcgactgt cattgatttg
     1861 ttgggtcacg cggcttaact caaggccgat cgccaccacg ccaatttgtt tatgattttc
     1921 atcgtagatg ggggtaaata cgcgtaaagc ctgcgccaga aaaccgcgat tgatagcgac
     1981 attttctttg ccattcagcg ctttaaggat gtcatcacct ttaaatggct gaccaatacg
     2041 ctgggcttca ggatgcgagt agcgaagact ttgcatatcg gtaacgacaa taaacagcag
     2101 atcgttgcgt ttgcgcacgg cttccgcgat ggcctggatt ccgctctcct gcggtttttt
     2161 ctgcaagccc tgacggattt ccggcgagtc ggcgagggta cgcgccactg ccagtgcctt
     2221 gttggccagc ccgtctcgcg tcatatcact gatttgcgag aagtaaatca gatgcaccac
     2281 caatagcacc gagaacagta ccgcgctgac cattaagatc actgtggtac tcaatttcat
     2341 cggacgtttg cgtaacatac ggtagggcaa tgaatgtctc atcagcttcc ttgtgtgaca
     2401 aatttcttaa gcattatctc tgatgaggcg ggtaattcaa agggagtaag aatgattggc
     2461 tatatagggg aagagactct ggcaacggaa actgccagtg ctgtatgaag attccagggc
     2521 tatgcttata gcgataatca tactgatgag agagggaaag tcatggatca ggcgctactg
     2581 gacgagggtt atcgctgtta taccggcgaa aagatcgatg tctatttcaa cactgcgata
     2641 tgtcagcatt ctggcaattg cgtacgtggc aacggcaagt tatttaatct caaacgaaag
     2701 ccgtggatca tgccggatga agtcgacgtc gtcactgtgg ttaaagtgat tgatacgtgc
     2761 ccgagcggcg cgctgaaata ccgtcataaa taagcgaggg taaaatggaa atacgcgaag
     2821 gccacaataa attttacatt aatgacgaac aaggcaagca aatcgctgaa attgtctttg
     2881 tgccgaccgg agagaattta gcgattatcg aacataccga tgtcgatgaa agcctgaaag
     2941 ggcaggggat tggtaaacag ctggttgcga aagtcgtgga aaaaatgcgt cgggaaaaac
     3001 gaaaaattat cccactatgc ccatttgcga aacacgaatt tgataaaacg cgggagtatg
     3061 atgatattcg cagttgatgg gagagtacag agtcacgata tttttcatgc tctccgcgga
     3121 gtgatgcagg agagcatctg aagggtaggg ggatgcacaa agaatgggca gagagcgcgt
     3181 ttttttgtcc caagtcatcc cctttactga gcaaaaaaaa gaatatctcc tatatgagaa
     3241 tcatcaatcg gggttaataa tttttgcgtc cccagagcat tcaatattga taggagtcat
     3301 attatggaag gtaaaaacaa gttcaatact tatgttgttt cttttgatta tccatcatct
     3361 tattcctcag tgttcttaag gttaagatca ttgatgtatg atatgaattt ctcttctatc
     3421 gtggctgatg aatatgggat accacgacaa ttgaatgaaa attccttcgc aataacgaca
     3481 tcgttagccg caagtgaaat cgaagattta atcaggctta aatgcttaga cttaccggat
     3541 attgattttg acctcaacat tatgacagtt gatgactatt tccgtcagtt ttacaagtag
     3601 ccaggaagga taagaagaac ata
//
LOCUS       DAZKHF020000334         3589 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2201S31241
            SAMN43267800-rid25106413.denovo.336, whole genome shotgun sequence.
ACCESSION   DAZKHF020000334 DAZKHF020000000
VERSION     DAZKHF020000334.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267800
            Sequence Read Archive: SRR30307263
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3589)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3589)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 3589)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350581.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350581.2
            Genome Coverage       :: 151x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:21:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,652
            CDSs (total)                      :: 5,557
            Genes (coding)                    :: 5,320
            CDSs (with protein)               :: 5,320
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 237
            CDSs (without protein)            :: 237
            Pseudo Genes (ambiguous residues) :: 0 of 237
            Pseudo Genes (frameshifted)       :: 102 of 237
            Pseudo Genes (incomplete)         :: 130 of 237
            Pseudo Genes (internal stop)      :: 52 of 237
            Pseudo Genes (multiple problems)  :: 41 of 237
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3589
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267800-rid25106413.denovo.336"
                     /serotype="O 26:H11"
                     /isolate="2201S31241"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-01"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            <1..1053
                     /gene="rfbB"
                     /locus_tag="ACHOSC_RS22495"
                     /old_locus_tag="pgaptmp_004499"
     CDS             <1..1053
                     /gene="rfbB"
                     /locus_tag="ACHOSC_RS22495"
                     /old_locus_tag="pgaptmp_004499"
                     /EC_number="4.2.1.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008460 - dTDP-glucose 4,6-dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009225 - nucleotide-sugar metabolic
                     process [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-glucose 4,6-dehydratase"
                     /protein_id="HGU4550295.1"
                     /translation="IGSAVVRHIINNTQDSVVNVDKLTYAGNLESLADVSDSERYVFE
                     HADICDAAAMARIFAQHQPDAVMHLAAESHVDRSITGPAAFIETNIVGTYVLLEAARN
                     YWSALDSDKKNSFRFHHISTDEVYGDLPHPDEVNNTEELPLFTETTAYAPSSPYSASK
                     ASSDHLVRAWKRTYGLPTIVTNCSNNYGPYHFPEKLIPLVILNALEGKALPIYGKGDQ
                     IRDWLYVEDHARALYTVVTEGKAGETYNIGGHNEKKNIDVVLTICDLLDEIVPKEQSY
                     REQITYVADRPGHDRRYAIDAEKISRELGWKPQETFESGIRKTVEWYLSNTKWVENVK
                     SGAYQSWIAQNYEGRQ"
     gene            1053..1952
                     /gene="rfbD"
                     /locus_tag="ACHOSC_RS22500"
                     /old_locus_tag="pgaptmp_004500"
     CDS             1053..1952
                     /gene="rfbD"
                     /locus_tag="ACHOSC_RS22500"
                     /old_locus_tag="pgaptmp_004500"
                     /EC_number="1.1.1.133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose reductase"
                     /protein_id="HGU4550296.1"
                     /translation="MNILLFGKTGQVGWELQRALAPLGNLIALDVHSTDYCGDFSNPE
                     GVAETVRSIRPDIIVNAAAHTAVDKAESEPEFAQLLNATSVEAIAKAANEVGAWLIHY
                     STDYVFPGNGDMPWRETDATAPLNVYGETKLAGEKALQEYCAKHLIFRTSWVYAGKGN
                     NFAKTMLRLAKEREELAVINDQFGAPTGAELLADCTAHAIRVALNKPEVAGLYHLVAS
                     GTTTWHDYAALVFEEARKAGIPLALNKLNAVPTTAYPTPARRPHNSRLNTEKFQQKFA
                     LVLPDWQVGVKRMLNELFTTTAI"
     gene            2010..2888
                     /gene="rfbA"
                     /locus_tag="ACHOSC_RS22505"
                     /old_locus_tag="pgaptmp_004501"
     CDS             2010..2888
                     /gene="rfbA"
                     /locus_tag="ACHOSC_RS22505"
                     /old_locus_tag="pgaptmp_004501"
                     /EC_number="2.7.7.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008879 - glucose-1-phosphate
                     thymidylyltransferase activity [Evidence IEA];
                     GO_process: GO:0045226 - extracellular polysaccharide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate thymidylyltransferase RfbA"
                     /protein_id="HGU4550297.1"
                     /translation="MKTRKGIILAGGSGTRLYPVTMAVSKQLLPIYDKPMIYYPLSTL
                     MLAGLRDILIISTPQDTPRFQQLLGDGSQWGLNLQYKVQPSPDGLAQAFIIGEEFIGG
                     DDCALVLGDNIFYGHDLPKLMDVAVNKESGATVFAYHVNDPERYGVVEFDKNGTAISL
                     EEKPLQPKSNYAVTGLYFYDNYVVEMAKNLKPSARGELEITDINRIYMEQGRLSVAMM
                     GRGYAWLDTGTHQSLIEASNFIATIEERQGLKVSCPEEIAYRKGFIDAEQVKVLAEPL
                     KKNAYGQYLLKMIKGY"
     gene            2893..3435
                     /gene="rfbC"
                     /locus_tag="ACHOSC_RS22510"
                     /old_locus_tag="pgaptmp_004502"
     CDS             2893..3435
                     /gene="rfbC"
                     /locus_tag="ACHOSC_RS22510"
                     /old_locus_tag="pgaptmp_004502"
                     /EC_number="5.1.3.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001100797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008830 - dTDP-4-dehydrorhamnose
                     3,5-epimerase activity [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose 3,5-epimerase"
                     /protein_id="HGU4550298.1"
                     /translation="MNVIKTEIPDVLIFEPKVFGDERGFFMESFNQKVFEEAVGRKVE
                     FVQDNHSKSSKGVLRGLHYQLEPYAQGKLVRCVVGEVFDVAVDIRKSSPTFGKWVGVN
                     LSAENKRQLWIPEGFAHGFLVLSEIAEFVYKTTNYYHPESEGCIKWDDSFLMIDWPNK
                     PIAISEKDKKGSSILRLNDY"
BASE COUNT         1021 a          672 c          870 g         1026 t
ORIGIN      
        1 attggttctg ctgtagttcg tcacattata aataatacgc aggatagtgt tgttaatgtc
       61 gataaattaa cgtacgccgg aaacctggaa tcacttgctg atgtttctga ttctgaacgc
      121 tatgtttttg aacatgcgga tatttgcgat gcagctgcaa tggcgcggat ttttgctcag
      181 catcagccgg atgcagtgat gcacctggct gctgaaagcc atgttgaccg ttcaattacc
      241 ggccctgcgg catttattga aaccaatatt gttggtactt atgtcctttt ggaagccgct
      301 cgcaattact ggtctgctct tgatagcgac aagaaaaata gcttccgttt tcatcatatt
      361 tctactgacg aagtctatgg tgatttgcct catcctgacg aagtaaataa tacagaagaa
      421 ttacccttat ttactgagac gacagcttac gcgccaagca gtccttattc cgcatccaaa
      481 gcatctagcg atcatttagt ccgcgcgtgg aaacgtacct atggtttacc gaccattgtg
      541 actaactgtt cgaataacta cggcccttat cactttccgg aaaaattgat tccgctggta
      601 attcttaatg ctctggaagg taaggcatta cctatttatg gcaaagggga tcaaattcgc
      661 gattggctat atgttgaaga tcatgcgcgt gcgttatata ccgttgtcac cgaaggtaaa
      721 gcgggtgaaa cttataacat tggcggacac aacgaaaaga aaaacatcga tgttgtgctg
      781 actatttgtg atttgttgga tgagattgta ccgaaagagc aatcttatcg tgagcaaatt
      841 acttatgttg ccgatcgtcc gggacacgat cgccgttatg cgattgatgc tgagaagatt
      901 agccgcgaat tgggctggaa accgcaggaa acgtttgaga gcgggattcg gaagacagtg
      961 gaatggtacc tgtccaatac aaaatgggtc gaaaatgtga aaagtggtgc ctatcagtca
     1021 tggattgcac agaactatga gggccgtcag taatgaatat cctccttttc ggcaaaacag
     1081 ggcaggtagg ttgggaacta cagcgtgctc tggcacctct gggtaatttg attgctcttg
     1141 atgttcactc tactgattat tgcggtgatt ttagtaatcc tgaaggtgta gctgaaaccg
     1201 taagaagcat tcggcctgat attattgtca acgcagccgc tcacaccgca gtagacaaag
     1261 cagaatcaga accggagttt gcacaattac ttaacgcaac aagtgtcgaa gcgattgcga
     1321 aagcagccaa tgaagtcggc gcctggctta ttcattactc gactgattac gtcttccctg
     1381 gaaatggcga tatgccatgg cgggagacgg atgcaaccgc accactaaat gtttacggtg
     1441 aaaccaagtt agccggagaa aaagcgttac aggaatattg cgcaaagcat cttattttcc
     1501 ggaccagctg ggtctatgca ggaaaaggaa ataacttcgc caaaacgatg ttacgtctgg
     1561 caaaagagcg tgaagaatta gcggttatta atgatcagtt tggtgcgcca acaggtgctg
     1621 aactgctggc tgattgtaca gcacatgcca ttcgtgtcgc actgaataaa ccagaagtcg
     1681 caggcttgta ccatctggta gccagtggta ccacaacctg gcacgattat gctgcgctgg
     1741 tttttgaaga ggcacgcaaa gcaggtattc cccttgcact caacaagctc aacgcagtac
     1801 caacaacagc ctatcctaca ccagctcgtc gtccgcataa ctctcgcctt aatacagaaa
     1861 aatttcagca gaaatttgcg cttgttttgc ctgattggca ggttggcgtg aaacgaatgc
     1921 tcaacgaatt atttacgact acagcaattt aatagttttt gcatcttgtt cgtgatggtg
     1981 gagcaagatg aattaaaagg aatgatgaaa tgaaaacgcg taaaggtatt attttagctg
     2041 gtggttcggg tactcgtctt tatcctgtaa ctatggctgt cagtaaacag ttgttaccga
     2101 tttatgataa accgatgatc tattacccgt tgtctacact gatgttagcg ggtcttcgcg
     2161 atattctgat tattagtacg ccacaggata ctcctcgttt tcaacaactg ctgggtgacg
     2221 ggagccagtg ggggctaaat cttcagtaca aagtgcaacc gagtccagat ggtcttgcgc
     2281 aggcatttat catcggtgaa gagtttatcg gtggtgatga ttgtgctttg gttctaggtg
     2341 ataatatctt ttacggtcac gatctgccga agttaatgga tgtcgctgtt aacaaagaaa
     2401 gtggtgcaac ggtatttgcc tatcacgtta atgatcctga acgctacggt gtcgttgagt
     2461 ttgataaaaa cggtacggcg atcagcctgg aagaaaaacc gctacaacca aaaagtaatt
     2521 atgcggtaac cgggctttat ttttatgata actacgttgt cgaaatggcg aaaaatctta
     2581 agccttctgc ccgcggtgaa ctggaaatta ccgatattaa ccgtatttat atggaacagg
     2641 ggcgtttatc cgttgccatg atgggacgtg gttatgcatg gctggacacg gggacacatc
     2701 aaagtcttat tgaggcaagc aattttatcg caacaataga agaacgtcag gggctgaaag
     2761 tttcctgccc ggaagaaatt gcttaccgta aagggtttat cgatgctgag caggtgaaag
     2821 tattagctga accgttgaag aaaaatgctt atggtcagta tctgctgaaa atgattaaag
     2881 gttattaata aaatgaacgt aattaaaaca gaaattccag atgtactgat ttttgaaccg
     2941 aaagtttttg gtgatgagcg tggtttcttt atggaaagct ttaatcagaa agtttttgaa
     3001 gaagctgtag ggcggaaggt tgaatttgtt caggataatc attctaaatc atctaagggt
     3061 gtgttacgcg gactgcacta tcagctggaa ccctatgctc aaggtaaatt agttcgttgt
     3121 gtggtcggtg aagtatttga tgtagcagtt gatattcgta aatcgtcacc tacatttggg
     3181 aaatgggttg gggtgaattt gtctgctgag aataagcgtc agttgtggat ccctgagggg
     3241 tttgcgcatg gttttttggt gttaagcgag atagcagagt ttgtttataa gacgacaaat
     3301 tattatcatc ctgaatcaga aggttgcata aaatgggatg attctttttt gatgattgat
     3361 tggccaaata aaccgatagc aatctcagaa aaagataaaa aaggctcatc aattttgagg
     3421 ctaaatgact attgatgttg aaaaaaaaac ttcaaaaaat aaaggaatat cattcagtat
     3481 tggagttggc aataattcag ggtgcgaatg ccatatttcc tgtgttggta ttcccatttt
     3541 ttcttattac cttaggggaa aacatctttt caagtattgc tgttggtga
//
LOCUS       DAZKHF020000335         3521 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2201S31241
            SAMN43267800-rid25106413.denovo.337, whole genome shotgun sequence.
ACCESSION   DAZKHF020000335 DAZKHF020000000
VERSION     DAZKHF020000335.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267800
            Sequence Read Archive: SRR30307263
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3521)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3521)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 3521)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350581.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350581.2
            Genome Coverage       :: 151x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:21:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,652
            CDSs (total)                      :: 5,557
            Genes (coding)                    :: 5,320
            CDSs (with protein)               :: 5,320
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 237
            CDSs (without protein)            :: 237
            Pseudo Genes (ambiguous residues) :: 0 of 237
            Pseudo Genes (frameshifted)       :: 102 of 237
            Pseudo Genes (incomplete)         :: 130 of 237
            Pseudo Genes (internal stop)      :: 52 of 237
            Pseudo Genes (multiple problems)  :: 41 of 237
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3521
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267800-rid25106413.denovo.337"
                     /serotype="O 26:H11"
                     /isolate="2201S31241"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-01"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            96..950
                     /locus_tag="ACHOSC_RS22515"
                     /old_locus_tag="pgaptmp_004503"
     CDS             96..950
                     /locus_tag="ACHOSC_RS22515"
                     /old_locus_tag="pgaptmp_004503"
                     /EC_number="4.1.2.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000275530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0009025 - tagatose-bisphosphate aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tagatose bisphosphate family class II aldolase"
                     /protein_id="HGU4550299.1"
                     /translation="MYIISSKNMLKKAQSEGYAVPAFNIHNLETLQVVVESAAQLRSP
                     VMLAGTPGTYRYGGVGSLISLVQSLAREYNLPLVLHLDHHEESDDIFNKVRAGIRSVM
                     IDGSHLPFEDNIARVQEVTTFCHRFDVSVEAELGRLGGQEDDLLVDEKDSAYTSPAAA
                     AEFVQRTQIDSLAVAIGTAHGLYAHEPRLDFVRLEQIRQRVEIPLVLHGASGLSEQDV
                     QHCIRRGICKVNVATELKIAFADALKGYFYNNPAANDPRHYMQPAKAAMKEVVTRIIG
                     VCGSEGKI"
     gene            986..1876
                     /locus_tag="ACHOSC_RS22520"
                     /old_locus_tag="pgaptmp_004504"
     CDS             986..1876
                     /locus_tag="ACHOSC_RS22520"
                     /old_locus_tag="pgaptmp_004504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001341904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HGU4550300.1"
                     /translation="MKNIKLLGSAMLVSLALFSQNSLAKEYKIGASLLTQQHPFYIDL
                     ANAMKEEAKKDNVNLSVSIANQDLNKQLSDVEDFITKKVDAIIISPVDSKGVQAAIIK
                     ADKAGIPVITVDVAAEGVPVVSHVATDNYAGGVEAGKLMGKLLNGKGTVAIISYPALQ
                     SVVARVDGFKKGLSDTPDIKIVAEQPGITRAEALTTAQNIMQANANLNGLFGFGDDAA
                     LAAVIAAKSAHNDNIKIIGFDGMKEARDAVDSEKTFAAVIRQYPDQMGAKAIDAAVDH
                     LNGKPVEKMIPVQPGVYTGK"
     gene            1937..3436
                     /locus_tag="ACHOSC_RS22525"
                     /old_locus_tag="pgaptmp_004505"
     CDS             1937..3436
                     /locus_tag="ACHOSC_RS22525"
                     /old_locus_tag="pgaptmp_004505"
                     /EC_number="7.5.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020245594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter ATP-binding protein"
                     /protein_id="HGU4550301.1"
                     /translation="MTDVILDVSHIAKTFGHVQALKDITLSLRKGRVHTLLGENGAGK
                     STLMKILAGVYPPTQGTITLRGETITINNPQHSRQLGIAIIFQELSLSNNMTVAENIY
                     ANNEPRRFGIINDKKMLADCQNLLAELGIPLDPLEMVGNMSMAHRQLVEIAKALSYAA
                     DVVIMDEPTSSLSDNEAEILFNIIEKLKQRGCAVIYISHRMDEIMRISDDISVIRDGE
                     YIATHEKKNSDIQHLIAQMVGREMKNIWPARLGEIPDENVPAKLEVKNLSHPSLFKEI
                     SFAVRPGEVLGFFGLVGAGRSDVMKALFGLVSYQGTVLIDGKEVRIANPKQAIDHGIA
                     FVTENRKEEGLVLMHDVNMNTHHVAFQYNASRMGLINHRQEEAKTLQSIARMNTKVSS
                     VHQAVGALSGGNQQKIVLSKWLEKTPRILLLDEPTRGVDVGAKFEIYNVIRQLAAAGT
                     AIILVSSELPEVMALSDRLVVMRNKTIADIYSCENLTQTQVMTAATGVR"
BASE COUNT          997 a          715 c          872 g          937 t
ORIGIN      
        1 tttgccgctt ttgctgtgct agtttgcttt caaaatgaaa gtgaatattc tctttcttca
       61 ttttgtaagg cgaacgacaa ataacaggag ctgcgatgta catcatttca agcaaaaaca
      121 tgctgaaaaa agcccagtca gagggatatg ccgtccccgc tttcaacatt cataaccttg
      181 aaactttaca ggtagttgtt gagagcgctg cacaacttcg ctctcccgtt atgctggcgg
      241 gaacacccgg tacttatcgt tatggtggcg tgggaagttt aatttcgtta gtacaatcgc
      301 tggctcgcga gtacaacctg ccgttagtcc tgcatctcga ccatcatgaa gagagtgatg
      361 acatttttaa caaggttcgc gcagggatcc gttctgtcat gattgatggc tcccatttgc
      421 catttgaaga caacattgcg cgagtgcaag aagttaccac cttttgccat cgctttgatg
      481 ttagcgttga ggccgagcta gggcgtcttg gcggccagga ggacgatctg ctggtggatg
      541 aaaaagattc cgcttatacc tcaccagccg cagctgcaga gtttgttcaa cgtacccaaa
      601 ttgactccct ggccgtagca atcggcacag cacatggttt atatgcgcat gaaccacgac
      661 tggattttgt gcggctggaa cagattcgtc agcgagtgga gattccactg gtgttgcatg
      721 gtgcttcggg cttatcagaa caggacgttc agcactgtat tcgccgcggt atttgtaaag
      781 tgaatgtcgc gacagaattg aaaattgcgt ttgccgatgc gttaaaagga tatttctaca
      841 acaatcctgc ggcaaatgat ccacgccact atatgcagcc cgcaaaggcc gccatgaaag
      901 aagtcgtaac tcgtattatt ggtgtctgtg gtagtgaagg aaaaatttaa gacgttgtac
      961 cctacaaaaa caatgaggat tgtttatgaa aaatattaaa ttacttggtt cagctatgtt
     1021 ggtgagcctg gcattgttct cgcaaaattc gttggcaaaa gagtataaaa ttggtgcgtc
     1081 attattaaca cagcaacatc ctttctatat tgatcttgcg aatgccatga aagaagaggc
     1141 aaaaaaagac aatgtcaatt taagtgtctc tattgccaat caggatttaa acaaacaact
     1201 ttctgatgtt gaggatttta ttactaaaaa agtggatgcc attattatct ctccggtcga
     1261 ttcgaaaggc gtccaggcgg cgataattaa agccgataag gccggtattc ccgttatcac
     1321 cgtcgacgtc gctgcggaag gcgttccggt cgtatcgcat gttgcgaccg ataactatgc
     1381 gggaggcgtt gaagcaggca agttaatggg caaacttttg aatggtaaag gtactgtagc
     1441 cattatttct tatccggcac tacaatcggt ggttgcgcgc gttgatggtt ttaagaaagg
     1501 gttaagcgat acaccggata ttaaaattgt tgctgaacag cctggtatta cgcgggctga
     1561 agcgttaacg actgcgcaaa acattatgca ggcaaatgcc aacctgaatg gtctgtttgg
     1621 ctttggtgat gatgcggcgc tggcagcggt tatcgcagca aaatctgcgc ataacgacaa
     1681 catcaaaatc attggttttg acggcatgaa agaagcgcgt gatgccgttg acagtgaaaa
     1741 aacgtttgcg gccgtgattc ggcaatatcc cgatcaaatg ggtgctaaag ccattgatgc
     1801 cgctgttgat cacctcaatg gtaaaccggt agaaaaaatg atcccggtac aacccggcgt
     1861 ttatacaggt aaatagtaaa catcttcacc tcacaacaaa acgttattgg cgttttgttg
     1921 tgacaccgag gtcattatga cggatgttat tcttgatgtt tcgcatattg cgaagacatt
     1981 tggtcatgtt caagcattaa aagatattac cctctcttta cgtaaagggc gggtgcatac
     2041 tttattaggc gagaatggtg ccggtaaatc gacattaatg aaaatactgg ccggtgttta
     2101 tccgccaacc cagggaacga taacactacg tggtgaaact atcaccatta ataatccgca
     2161 gcattcacgc cagttaggaa ttgcgattat ttttcaggaa ttgagcctga gtaataatat
     2221 gaccgtggcg gaaaatattt atgccaataa tgaaccgcga cgttttggca ttattaacga
     2281 taaaaaaatg ctcgcagatt gccagaatct cctcgcggaa ctgggcatac cactagaccc
     2341 actagaaatg gtcggcaata tgtcgatggc acataggcaa ctggtagaaa ttgctaaagc
     2401 cttaagctat gcagcggatg tcgtgattat ggatgaaccg acatcttcgc taagtgataa
     2461 tgaagcggag attctgttta acattattga aaaattaaag cagcgaggct gtgcggttat
     2521 ttatatctct catcgcatgg atgaaattat gcggatttca gatgatattt cggtgatccg
     2581 cgacggtgag tatatcgcca cgcatgaaaa gaaaaacagt gatatccagc atttgattgc
     2641 ccaaatggtt ggtcgggaaa tgaaaaatat ttggccagca cgtcttgggg aaataccgga
     2701 tgaaaatgtt cctgcaaaac tggaagtaaa aaatctttcc catccttcgc tttttaaaga
     2761 gatcagtttc gctgtgcgtc cgggggaggt cctcggattt tttggcctgg tgggggccgg
     2821 gcgttccgat gtgatgaagg cgctctttgg cctcgtttcg tatcagggga ctgtgttgat
     2881 tgatggtaag gaagtcagga tcgctaatcc taaacaggct attgatcatg gcattgcttt
     2941 tgtgaccgaa aaccgcaagg aagaagggtt agtgctgatg cacgacgtca atatgaacac
     3001 gcaccatgtg gcgtttcaat ataacgcttc gcggatggga ctcatcaatc accgacaaga
     3061 agaggcgaag acgctgcaat caattgccag aatgaacact aaagtgagtt ctgtacatca
     3121 ggcagtggga gcgctgagcg gcggtaatca acagaaaatc gtgttatcga agtggctgga
     3181 gaaaactccg cgtattttat tgctcgatga gccaacgcgc ggagtggatg tcggcgcaaa
     3241 atttgaaatc tataacgtga tacgtcaact ggctgcggca gggacagcaa ttattctggt
     3301 gtcctctgaa ctgccagaag tcatggcact tagcgatcgt ctggtggtaa tgcgtaataa
     3361 aacgattgcc gatatttatt catgtgaaaa tttaacgcag acacaggtaa tgaccgctgc
     3421 aacaggagta cgttaaatga cagagaaaac tcttgccagt ccgaaacctt cggaaattcc
     3481 cattgcaaat aattttaaaa aagtcttcag gcaatatggc g
//
LOCUS       DAZKHF020000336         3505 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2201S31241
            SAMN43267800-rid25106413.denovo.338, whole genome shotgun sequence.
ACCESSION   DAZKHF020000336 DAZKHF020000000
VERSION     DAZKHF020000336.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267800
            Sequence Read Archive: SRR30307263
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3505)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3505)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 3505)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350581.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350581.2
            Genome Coverage       :: 151x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:21:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,652
            CDSs (total)                      :: 5,557
            Genes (coding)                    :: 5,320
            CDSs (with protein)               :: 5,320
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 237
            CDSs (without protein)            :: 237
            Pseudo Genes (ambiguous residues) :: 0 of 237
            Pseudo Genes (frameshifted)       :: 102 of 237
            Pseudo Genes (incomplete)         :: 130 of 237
            Pseudo Genes (internal stop)      :: 52 of 237
            Pseudo Genes (multiple problems)  :: 41 of 237
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3505
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267800-rid25106413.denovo.338"
                     /serotype="O 26:H11"
                     /isolate="2201S31241"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-01"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            <1..629
                     /gene="fimC"
                     /locus_tag="ACHOSC_RS22530"
                     /old_locus_tag="pgaptmp_004506"
     CDS             <1..629
                     /gene="fimC"
                     /locus_tag="ACHOSC_RS22530"
                     /old_locus_tag="pgaptmp_004506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="type 1 fimbria chaperone FimC"
                     /protein_id="HGU4550302.1"
                     /translation="AAGGIALGATRIIYPADAKQTAVWIRNSHTNERFLVNSWIENSS
                     GVKEKSFIITPPLFVSEPKSENTLRIIYTGPPLAADRESLFWMNVKTIPSVDKNALNG
                     RNVLQLAILSRMKLFLRLIQLQELPAEAPDTLKFSRSGNYINVHNPSPFYVTLVNLQV
                     GSQKLGNAMAAPRVNSQIPLPSGVQGKLKFQTVNDYGSVTPVREVNLN"
     gene            660..3269
                     /locus_tag="ACHOSC_RS22535"
                     /old_locus_tag="pgaptmp_004507"
     CDS             660..3269
                     /locus_tag="ACHOSC_RS22535"
                     /old_locus_tag="pgaptmp_004507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0015473 - fimbrial usher porin activity
                     [Evidence IEA];
                     GO_process: GO:0009297 - pilus assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial biogenesis usher protein"
                     /protein_id="HGU4550303.1"
                     /translation="MKIPTTTDIPQRYTWCLAGICYSSLAILPSFLSYAESYFNPAFL
                     LENGTSVADLSRFERGNHQPAGVYRVDLWRNDEFIGSQDIVFESTTENTGDKSGGLMP
                     CFNQVLLERISLNSSAFPELAQQQNNKCINLLKAVPDATINFDFAAMRLNITIPQIAL
                     LSSAHGYIPPEEWDEGIPALLLNYNFTGNRGNGNDSYFFSELSGINIGPWRLRNNGSW
                     NYFRGNGYHSEQWNNIGTWVQRAIIPLKSELVMGDGNTGSDIFDGVGFRGIRLYSSDN
                     MYPDSQQGFAPTVRGIARTAAQLTIRQNGFIIYQSYVSPGAFEITDLHPTSSNGDLDV
                     TIDERDGNQQNYTIPYSTVPILQREGRFKFDLTAGDFRSGNSQQSSPFFFQGTALGGL
                     PQEFTAYGGTQLSANYTAFLLGLGRNLGNWGTVSLDVTHARSQLADDSRHEGDSIRFL
                     YAKSMNTFGTNFQLMGYRYSTQGFYTLDDVAYRRMEGYEYDYDYDGEHRDEPIIVNYH
                     NLRFSRKDRLQLNISQSLNDFGSLYISGTHQKYWNTSDSDTWYQVGYTSSWVGISYSL
                     SFSWNESVGIPDNERIVGLNVSVPFNVLTKRRYTRENALDRAYASFNANRNSNGQNSW
                     LAGVGGTLLEGHNLSYHVSQGDTSNNGYTGSATANWQAAYGTLGVGYNYDRDQHDVNW
                     QLSGGVVGHENGITLSQPLGDTNVLIKAPGAGGVRIENQTGILTDWRGYAVMPYATVY
                     RYNRIALDTNTMGNSIDVEKNISSVVPTQGALVRANFDTRIGVRALITVTQGRKPVPF
                     GSLVRENSTGITSMVGDDGQVYLSGAPLSGELLVQWGDGANSRCIAHYVLPKQSLQQA
                     VTVISAVCTHPGS"
     gene            3311..>3505
                     /locus_tag="ACHOSC_RS22540"
                     /old_locus_tag="pgaptmp_004508"
     CDS             3311..>3505
                     /locus_tag="ACHOSC_RS22540"
                     /old_locus_tag="pgaptmp_004508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016155910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HGU4550304.1"
                     /translation="MACLCLANISWATVCANSTGVAEDEHYDLSNIFNSTNNQPGQIV
                     VLPEKSGWVGVSAICPPGTLV"
BASE COUNT          925 a          776 c          853 g          951 t
ORIGIN      
        1 atgctgccgg agggatcgca ttaggtgcca cgcgtattat ttatcccgct gatgctaaac
       61 agactgcggt atggattaga aatagccata ccaatgagcg ctttctggtc aattcgtgga
      121 ttgaaaacag cagcggtgta aaagaaaagt cattcatcat tacaccgcca ctgtttgtta
      181 gtgaacccaa aagcgaaaat accttgcgta ttatttacac cggtccaccg ctggcagcag
      241 atcgtgagtc tctgttctgg atgaatgtta agacgatccc ttcggtagat aaaaatgcat
      301 tgaacggcag gaatgttttg caactggcga ttttatcgcg catgaaatta tttctccgtc
      361 taattcaatt acaagaatta cccgcagaag caccggacac actcaagttt tcgcgatccg
      421 gtaactatat caatgttcat aatccctcac ctttttatgt cactctggtt aacttacaag
      481 tgggcagcca aaagttgggg aatgctatgg ctgcacccag agttaattca caaattccct
      541 taccctcagg agtgcaggga aagctgaaat ttcagaccgt taatgattat ggttcagtaa
      601 ccccggtcag agaagtgaac ttaaactaac cgaatcatct gacaatatca gagctaatta
      661 tgaaaatacc cactactacg gatattccgc agaggtatac ctggtgtctg gccggaattt
      721 gttattcatc tcttgccatt ttaccctcct ttttaagcta tgcggaaagt tatttcaacc
      781 cggcattttt attagagaat ggcacatccg ttgctgattt atcgcgcttt gagagaggta
      841 atcatcaacc tgcgggcgtg tatcgggtag atctctggcg taatgatgag ttcattggtt
      901 cacaggatat cgtatttgaa tcgacaacag aaaatacagg tgataaatca ggtgggttaa
      961 tgccctgttt taaccaggta ctccttgaac gaattagcct taatagcagt gcatttcccg
     1021 agttagccca gcagcaaaac aataaatgca tcaatttact gaaagctgta cctgatgcca
     1081 caattaactt tgattttgca gcgatgcgcc tgaacatcac tattcctcag atagcgttgt
     1141 tgagtagcgc tcacggttac attccgcctg aagagtggga tgaaggtatt cctgctttac
     1201 tcctgaatta taatttcacc ggtaacagag gtaatggtaa cgatagctat ttttttagtg
     1261 agctcagcgg gattaatatt ggcccgtggc gtttacgcaa caatggttcc tggaactatt
     1321 ttcgcggaaa tggatatcat tcagaacagt ggaataatat tggcacctgg gtacagcgcg
     1381 ccattattcc gctgaaaagt gaactggtaa tgggagacgg caatacagga agtgatattt
     1441 tcgatggtgt tggatttcgt ggtatacggc tttactcttc cgataatatg tatcctgata
     1501 gccagcaagg gtttgcccca acagtacgtg ggattgcccg tacggcggcc cagctaacga
     1561 ttcggcaaaa tggttttatt atctatcaaa gctatgtttc ccccggcgct tttgaaatta
     1621 cagatttgca cccgacatct tcaaatggcg atctggacgt caccatcgac gagcgcgatg
     1681 gtaatcagca gaattacaca attccgtatt caacagtgcc aattttacaa cgcgaagggc
     1741 gtttcaaatt tgacctgacg gcgggcgatt ttcgtagcgg taatagtcag caatcatcgc
     1801 ctttcttttt tcagggtacg gcactcggcg gtttaccaca ggaatttact gcctacggcg
     1861 ggacgcaatt atctgccaat tacaccgcct ttttattagg gctggggcgc aacctcggga
     1921 actggggcac agtgtcgctg gatgtgaccc atgcgcgcag tcagttagcc gacgacagtc
     1981 gtcatgaggg ggattccatt cgcttcctct atgcgaaatc gatgaacacc ttcggcacca
     2041 attttcagtt aatgggttac cgctattcga cacaaggttt ttataccctt gatgatgttg
     2101 cgtatcgtcg aatggagggg tacgaatatg attacgatta cgacggtgag catcgcgatg
     2161 aaccgataat cgtgaattac cacaatttac gctttagccg taaagaccgt ttgcagttaa
     2221 atatttcaca atcacttaat gactttggct cgctttatat ctctggtacc catcaaaaat
     2281 actggaatac ttcggattca gatacctggt atcaggtggg gtataccagc agctgggttg
     2341 gcatcagtta ttcactctca ttttcgtgga atgaatctgt agggatcccc gataacgaac
     2401 gtattgtcgg acttaatgtt tcagtgcctt tcaatgttct gaccaaacgt cgctacaccc
     2461 gggaaaatgc gctcgaccgc gcttatgcct cctttaacgc caaccgtaac agcaacgggc
     2521 aaaatagctg gctggcaggt gtaggtggga ccttactgga aggccacaac ctgagttatc
     2581 acgtaagcca gggtgatacc tcgaataatg ggtatacggg cagcgccacg gcaaactggc
     2641 aggccgctta cggtacgctg ggggtcgggt ataactacga ccgcgatcaa catgacgtta
     2701 actggcagct gtctggcggt gtggtcgggc atgaaaatgg cataacgctg agccagcctt
     2761 taggggatac caatgttttg attaaagcgc ctggcgcagg cggtgtacgc attgaaaatc
     2821 aaactggcat tttaaccgac tggcgcggct atgcggtgat gccgtatgcc acggtttatc
     2881 ggtataaccg tatcgcgctt gataccaata cgatggggaa ttccatcgat gttgaaaaaa
     2941 atattagcag cgttgtgccg acgcaaggcg cgttggttcg tgccaatttt gatacccgca
     3001 taggcgtgcg ggcgctcatt accgttaccc agggcagaaa accggtgccg tttggatcac
     3061 tggtacggga aaacagtacc ggaataacca gtatggtggg tgatgacggg caagtttatt
     3121 taagtggtgc gccattgtct ggtgaattac tggttcagtg gggagacggc gcgaactcac
     3181 gctgcattgc gcactatgta ttgccgaagc aaagcttaca gcaagccgtc actgttattt
     3241 cggcagtttg cacacatcct ggctcataaa ggaaattatc aataagataa tctgcagatt
     3301 attattggcg atggcatgtt tgtgtctggc aaacatatcc tgggctactg tttgtgcaaa
     3361 tagtactggc gtagcagaag atgaacacta tgatctctca aatatcttta atagcaccaa
     3421 taaccagcca gggcagattg ttgttttacc ggaaaaatcc ggctgggtag gtgtctcagc
     3481 aatttgtcca cccggtacgc tggtg
//
LOCUS       DAZKHF020000337         3490 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2201S31241
            SAMN43267800-rid25106413.denovo.339, whole genome shotgun sequence.
ACCESSION   DAZKHF020000337 DAZKHF020000000
VERSION     DAZKHF020000337.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267800
            Sequence Read Archive: SRR30307263
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3490)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3490)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 3490)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350581.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350581.2
            Genome Coverage       :: 151x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:21:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,652
            CDSs (total)                      :: 5,557
            Genes (coding)                    :: 5,320
            CDSs (with protein)               :: 5,320
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 237
            CDSs (without protein)            :: 237
            Pseudo Genes (ambiguous residues) :: 0 of 237
            Pseudo Genes (frameshifted)       :: 102 of 237
            Pseudo Genes (incomplete)         :: 130 of 237
            Pseudo Genes (internal stop)      :: 52 of 237
            Pseudo Genes (multiple problems)  :: 41 of 237
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3490
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267800-rid25106413.denovo.339"
                     /serotype="O 26:H11"
                     /isolate="2201S31241"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-01"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            complement(246..542)
                     /gene="yciI"
                     /locus_tag="ACHOSC_RS22545"
                     /old_locus_tag="pgaptmp_004509"
     CDS             complement(246..542)
                     /gene="yciI"
                     /locus_tag="ACHOSC_RS22545"
                     /old_locus_tag="pgaptmp_004509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415767.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YciI family protein"
                     /protein_id="HGU4550305.1"
                     /translation="MLYVIYAQDKADSLEKRLSVRPAHLARLQLLHDEGRLLTAGPMP
                     AVDSNDPGAAGFTGSTVIAEFESLEAAQAWADADPYVAAGVYEHVSVKPFKKVF"
     gene            766..1485
                     /gene="tonB"
                     /locus_tag="ACHOSC_RS22550"
                     /old_locus_tag="pgaptmp_004510"
     CDS             766..1485
                     /gene="tonB"
                     /locus_tag="ACHOSC_RS22550"
                     /old_locus_tag="pgaptmp_004510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB system transport protein TonB"
                     /protein_id="HGU4550306.1"
                     /translation="MTLDLPRRFPWPTLLSVCIHGAVVAGLLYTSVHQVIELPAPAQP
                     ISVTMVAPADLEPPQAVQPPPEPVVEPEPEPEPIPEPPKEAPVVIEKPKPKPKPKPKP
                     VKKVQEQPKRDIKPVESRPASPFENTAPARPTSSTATAATSKPVTSVASGPRALSRNQ
                     PQYPARAQALRIEGQVKVKFDVTPDGRVDNVQILSAKPANMFEREVKNAMRRWRYEPG
                     KPGSGIVVNILFKINGTTEIQ"
     gene            complement(1525..1923)
                     /gene="yciA"
                     /locus_tag="ACHOSC_RS22555"
                     /old_locus_tag="pgaptmp_004511"
     CDS             complement(1525..1923)
                     /gene="yciA"
                     /locus_tag="ACHOSC_RS22555"
                     /old_locus_tag="pgaptmp_004511"
                     /EC_number="3.1.2.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016790 - thiolester hydrolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioester hydrolase YciA"
                     /protein_id="HGU4550307.1"
                     /translation="MSTTHNVPQGDLVLRTLAMPADTNANGDIFGGWLMSQMDIGGAI
                     LAKEIAHGRVVTVRVEGMTFLRPVAVGDVVCCYARCVQKGTTSVSINIEVWVKKVASE
                     PIGQRYKATEALFKYVAVDPEGKPRALPVE"
     gene            complement(2028..2567)
                     /gene="yciB"
                     /locus_tag="ACHOSC_RS22560"
                     /old_locus_tag="pgaptmp_004512"
     CDS             complement(2028..2567)
                     /gene="yciB"
                     /locus_tag="ACHOSC_RS22560"
                     /old_locus_tag="pgaptmp_004512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septation protein A"
                     /protein_id="HGU4550308.1"
                     /translation="MKQFLDFLPLVVFFAFYKIYDIYAATAALIVATAIVLIYSWVRF
                     RKVEKMALITFVLVVVFGGLTLFFHNDEFIKWKVTVIYALFAGALLVSQWVMKKPLIQ
                     RMLGKELTLPQPVWSKLNLAWAVFFILCGLANIYIAFWLPQNIWVNFKVFGLTALTLI
                     FTLLSGIYIYRHMPQEDKS"
     gene            complement(2597..3340)
                     /gene="yciC"
                     /locus_tag="ACHOSC_RS22565"
                     /old_locus_tag="pgaptmp_004513"
     CDS             complement(2597..3340)
                     /gene="yciC"
                     /locus_tag="ACHOSC_RS22565"
                     /old_locus_tag="pgaptmp_004513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YciC family protein"
                     /protein_id="HGU4550309.1"
                     /translation="MSITAQSVYRDTGNFFRNQFMTILLVSLLCAFITVVLGHVFSPS
                     DAQLAQLNDGVPVSGSSGLFDLVQNMSPEQQQILLQASAASTFSGLIGNAILAGGVIL
                     IIQLVSAGQRVSALRAIGASAPILPKLFILIFLTTLLVQIGIMLVVVPGIIMAILLAL
                     APVMLVQDKMGIFASMRSSMRLTWANMRLVAPAVLSWLLAKTLLLLFASSFAALTPEI
                     GAVLANTLSNLISAILLIYLFRLYMLIRQ"
     gene            complement(3368..>3490)
                     /locus_tag="ACHOSC_RS22570"
                     /old_locus_tag="pgaptmp_004514"
     CDS             complement(3368..>3490)
                     /locus_tag="ACHOSC_RS22570"
                     /old_locus_tag="pgaptmp_004514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_460692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4550310.1"
                     /translation="CRGLPTPEGQQLPPLYKDMLFHTRPDAATRGLTRVQLPAN"
BASE COUNT         1008 a          861 c          831 g          790 t
ORIGIN      
        1 gaacatacat gcagcgcgtg aatgtgttaa acaatagtag atgactttat cggcagtaat
       61 caaaggtttt gagcgagcaa ctctgcgtca ggaaagggca ttttcctgaa tatgttcaca
      121 tcatataaag aaatacagat aaagttatta tctgcttgtg gtggtgaatg cactgaccgg
      181 ctataaggaa aggccaaaca agaacacggt tgcaaaaacc gtgcccttaa atattgaatc
      241 tctattcaga acactttctt aaatggtttc actgaaacgt gttcatagac tcctgccgct
      301 acgtacgggt cagcatcggc ccaggcctga gctgcttcca gcgattcaaa ttcagcaata
      361 acggttgagc cagtaaatcc cgcagcccct ggatcgttac tgtctaccgc tggcattgga
      421 ccagctgtca acaaacgacc ttcatcatgc agtaactgta aacgtgctaa atgtgccgga
      481 cgaacggaaa ggcgtttttc gaggctatca gctttgtctt gagcgtaaat aacatacaac
      541 acgggcacaa ctccttgttc gggaaagttg taaagtacgt tatttgaaag ggcgaagatc
      601 tgcaacggaa agatgatgtc tttgttaaga ccatgcataa attaaggtca attacgccaa
      661 aaatgacatt ttcactgatc ctgatcgtct tgccttattg aatatgattg ctatttgcat
      721 ttaaaatcga gacctggttt ttctactgaa atgattatga cttcaatgac ccttgattta
      781 cctcgccgct tcccctggcc gacgttactt tcggtctgca ttcatggtgc tgttgtggcg
      841 ggtctgctct atacctcggt acatcaggtt attgaactac ctgcgcctgc gcagccaatt
      901 tctgtcacga tggttgcgcc tgctgatctc gaaccgccac aagccgttca gccgccaccg
      961 gagccggtgg tagagccaga accggaacct gagccgatcc ccgaaccgcc aaaagaagca
     1021 ccggtggtca ttgaaaagcc gaagccgaaa cctaagccaa aaccgaagcc ggtgaaaaag
     1081 gtacaggagc agccaaaacg cgatatcaaa cccgtagagt cgcgtccggc atcaccgttt
     1141 gaaaatacgg cacccgcgcg ccccacatca agtacggcaa cggctgcaac cagcaagccg
     1201 gttaccagtg tggcttcagg accacgcgca ttaagccgta atcagccgca gtatccggca
     1261 cgagcacagg cattgcgcat tgaagggcag gttaaagtta aatttgacgt cacgccggat
     1321 ggtcgcgtgg ataacgtaca aatcctctca gccaagcctg cgaacatgtt tgagcgtgag
     1381 gtgaaaaatg cgatgcgcag atggcgttat gagccgggta agccaggcag tgggattgtg
     1441 gtgaatatcc tgtttaaaat taacggcacc accgaaattc agtaagcaga aagtcaaaag
     1501 cctccgaccg gaggcttttg actattactc aacaggtaag gcgcgaggtt ttccttcagg
     1561 atcaaccgcg acatacttaa ataatgcttc tgtcgcttta tagcgttgcc cgattggttc
     1621 agacgctact tttttcaccc acacttcaat attaatgctg accgatgtcg tccctttctg
     1681 gacacagcgt gcatagcagc acaccacatc gccgaccgca accggccgta agaaagtcat
     1741 tccttcaacc cgcacagtca ctacgcgacc gtgggcaatt tctttcgcca gaatagcgcc
     1801 gccaatatcc atttgtgaca ttaaccaacc accaaagatg tcaccattgg cattggtatc
     1861 ggcgggcatg gctaaagtac gtaaaacaag atcgccctga gggacgttat gtgttgtaga
     1921 catggtaaaa ccgacttaaa aggaaaatca caggcgcgat gctactatga ttttcctgag
     1981 gagaacagag gagatacagc gccagccccg aaggactggc agtctggtta ggatttatct
     2041 tcctgcggca tgtggcggta gatatagata ccgcttaaca atgtgaagat taaggtaagg
     2101 gcggtcaggc caaagacttt aaagttgacc caaatatttt gcggcagcca gaatgcgatg
     2161 tagatgtttg ccagaccgca aaggataaag aaaacagccc aggccagatt tagcttcgac
     2221 cataccggtt gcggcagcgt gagttcttta cccagcatcc gctgaattag cggctttttc
     2281 atcacccatt ggctgactaa cagggctccc gcaaacagag cataaatgac tgtaaccttc
     2341 catttaataa actcatcatt gtggaagaac agcgtcaagc caccgaagac gaccaccaga
     2401 acaaaagtga tcagggccat cttctcaacc ttacgaaagc gaacccagct atagataagc
     2461 acaatcgccg tggcgacgat cagcgccgca gtagccgcat agatgtcata aatcttgtaa
     2521 aacgcgaaaa agacaaccag cggtaaaaaa tcaagaaact gcttcatttt acgattccgt
     2581 aatcaaatgc ttaaggttat tggcgaatca acatatacag gcggaacaga tagatgagca
     2641 atatggcaga aatcaggttg ctcaaggtgt tcgccagtac ggcaccaatt tccggggtta
     2701 atgcggcaaa agaagaggca aaaagcagca acagtgtttt tgccagcaac cagctcagta
     2761 ctgcgggtgc caccagacgc atattcgccc aagtcagccg catactgcta cgcatcgagg
     2821 caaaaatgcc cattttgtcc tgtaccagca tcaccggtgc cagggccagt aaaatggcca
     2881 tgataattcc cggaacgacc accagcatga tgccaatctg tactaaaagg gtagtcagaa
     2941 aaatcagaat aaataacttg ggcaatatcg gcgcactggc accaatagct cgcagcgcac
     3001 tgactctctg acccgcagac accagctgga taattaatat tacgcctccg gcgagaatgg
     3061 cgttaccgat taatcctgaa aaagtggacg ccgctgaagc ctgcagcaaa atttgttgct
     3121 gttccggtga catattctga accaggtcga acaacccact actgccgcta acgggcacgc
     3181 cgtcattgag ctgcgcaagc tgtgcatcac tgggtgagaa aacatgccct aacaccactg
     3241 tgataaacgc acatagcaac gataccaaca gaatggtcat aaattgatta cggaagaaat
     3301 ttccggtgtc acggtatacg gactgcgccg tgatagacat gcactctcct tgagttttat
     3361 gcaggtgtta attagcgggc aattgtaccc tggttaagcc tcgggtggca gcatcaggtc
     3421 ttgtatggaa aagcatatct ttgtaaagcg gaggtaattg ctggccttca ggcgtgggca
     3481 aaccgcggca
//
LOCUS       DAZKHF020000338         3464 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2201S31241
            SAMN43267800-rid25106413.denovo.340, whole genome shotgun sequence.
ACCESSION   DAZKHF020000338 DAZKHF020000000
VERSION     DAZKHF020000338.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267800
            Sequence Read Archive: SRR30307263
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3464)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3464)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 3464)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350581.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350581.2
            Genome Coverage       :: 151x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:21:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,652
            CDSs (total)                      :: 5,557
            Genes (coding)                    :: 5,320
            CDSs (with protein)               :: 5,320
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 237
            CDSs (without protein)            :: 237
            Pseudo Genes (ambiguous residues) :: 0 of 237
            Pseudo Genes (frameshifted)       :: 102 of 237
            Pseudo Genes (incomplete)         :: 130 of 237
            Pseudo Genes (internal stop)      :: 52 of 237
            Pseudo Genes (multiple problems)  :: 41 of 237
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3464
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267800-rid25106413.denovo.340"
                     /serotype="O 26:H11"
                     /isolate="2201S31241"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-01"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            <1..420
                     /locus_tag="ACHOSC_RS22575"
                     /old_locus_tag="pgaptmp_004515"
     CDS             <1..420
                     /locus_tag="ACHOSC_RS22575"
                     /old_locus_tag="pgaptmp_004515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="minor capsid protein E"
                     /protein_id="HGU4550311.1"
                     /translation="VWRLLNGFKLFREKLDTRRGSNSQLETAVKDLGAVVSFKGYYGD
                     LAIVVAKTSYVAEDGTEKRYLPEGTLVLGNTAADGIRCYGAIQDAQALSEGVVASSRY
                     PKHWLTVGDPAREFTMTQSAPLMVLPDPDEFVVVQVK"
     gene            472..855
                     /gene="gpFI"
                     /locus_tag="ACHOSC_RS22580"
                     /old_locus_tag="pgaptmp_004516"
     CDS             472..855
                     /gene="gpFI"
                     /locus_tag="ACHOSC_RS22580"
                     /old_locus_tag="pgaptmp_004516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001355131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-packaging protein FI"
                     /protein_id="HGU4550312.1"
                     /translation="MATKEENQKHLRQLAGLLGREADMSGSAADIAQRVSEWEEELAA
                     SREGIMPGDESGPEQNHTDDGEQLHNTDATDDVKAVRVRKCLHVMGYCPETGRPVELT
                     YRGMRVLVPSPLATAMIQHGTAEHA"
     gene            848..1201
                     /locus_tag="ACHOSC_RS22585"
                     /old_locus_tag="pgaptmp_004517"
     CDS             848..1201
                     /locus_tag="ACHOSC_RS22585"
                     /old_locus_tag="pgaptmp_004517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001204533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="head-tail joining protein"
                     /protein_id="HGU4550313.1"
                     /translation="MRDFQNAFDAALAGVDSTIVEVMGLCAQFTSGAQCGSEVQGVFD
                     DPESLGFAGSGVRIEGSCPSLFVRTDTVRAVRRGDTLTINGETFWVDRVSPDDGGSCY
                     LWLNRGQPPAVNRRR"
     gene            1216..1749
                     /locus_tag="ACHOSC_RS22590"
                     /old_locus_tag="pgaptmp_004518"
     CDS             1216..1749
                     /locus_tag="ACHOSC_RS22590"
                     /old_locus_tag="pgaptmp_004518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HGU4550314.1"
                     /translation="MAIKGLDQAIENLSRVRKNAIPAASAMAINRVATTAINQSSSQV
                     ARETRVSRKLVKERSRLKRATVRNPNARIIVNRGDLPVIKLGIRMPGRRPDSILKAGQ
                     HRYQRAFIQRLKNGRWHVMQRVAGKNRYPIDVVKIPMAAPLKQAFDENVDRIRRERLP
                     GELAYALKQQLRIAIKR"
     gene            1746..2141
                     /locus_tag="ACHOSC_RS22595"
                     /old_locus_tag="pgaptmp_004519"
     CDS             1746..2141
                     /locus_tag="ACHOSC_RS22595"
                     /old_locus_tag="pgaptmp_004519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage minor tail U family protein"
                     /protein_id="HGU4550315.1"
                     /translation="MKHTDIRAAVLDALELHEHGATLFDGRPVVFDEEDFPAVAVYLT
                     DAEYTGEELDADTWRATLHIEVFLPAQVPDSELDSWMESRIYPAMSAIPALAGMITTM
                     VQQGYDYRRDDDMALWSSADLTYSITYEM"
     gene            2149..2901
                     /locus_tag="ACHOSC_RS22600"
                     /old_locus_tag="pgaptmp_004520"
     CDS             2149..2901
                     /locus_tag="ACHOSC_RS22600"
                     /old_locus_tag="pgaptmp_004520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HGU4550316.1"
                     /translation="MPTPNPLAPVKGAGTTLWVYNGQGDAYANPLSDDDWQRLAKVKD
                     LTPGEMTAESYDDNYLDDEDADWSATGQGQKSAGDTSFTLAWKPGEEGQKGLIGWFES
                     GDVRAYKIRFPNGTVDVFRGWISSIGKAVTAKEVITRTVKVTNVGKPSVAEERSEITP
                     ATAIKVTPTSGTVEKGKTTTLTVSFEPESATDKTFRAVSADPSTGTIAVKDMVITVTG
                     VKAGKVSIPVISGNGQFATVAEVTVTEAGAAG"
     gene            2915..3346
                     /locus_tag="ACHOSC_RS22605"
                     /old_locus_tag="pgaptmp_004521"
     CDS             2915..3346
                     /locus_tag="ACHOSC_RS22605"
                     /old_locus_tag="pgaptmp_004521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage minor tail protein G"
                     /protein_id="HGU4550317.1"
                     /translation="MFLKTEQFEYNGVSVTLSELSALQRIEHLALLKRRAEQAESSGN
                     LQVSVEDLVRTGAFLVAMSLWHNHPQKTGSPSMNEAVMQIEQEVLTTWPADAIARAED
                     VVLRLSGMSGPVHVDTDITEVAKNNALTDDDFSAGKSSTAS"
     gene            3373..>3464
                     /locus_tag="ACHOSC_RS22610"
                     /old_locus_tag="pgaptmp_004522"
     CDS             3373..>3464
                     /locus_tag="ACHOSC_RS22610"
                     /old_locus_tag="pgaptmp_004522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail assembly protein T"
                     /protein_id="HGU4550318.1"
                     /translation="MGRPDWRAMLAGMTSTEYADWRHFYRMHYF"
BASE COUNT          804 a          794 c         1098 g          768 t
ORIGIN      
        1 gtctggcgtc tgctgaatgg ttttaagctg ttccgcgaaa aactggatac ccgtcgcggc
       61 tcaaattcac aactcgaaac ggcagtgaaa gacctggggg cggtggtgtc tttcaaaggg
      121 tattacggcg atctggccat tgtggtggcg aaaacgtctt atgtggcaga ggacggtacc
      181 gaaaaacgtt atctgccgga gggcacgctg gtcctgggaa atacggctgc agatgggatc
      241 cgttgttacg gtgccattca ggatgcgcag gcgttgtccg aaggtgtggt ggcttcttcc
      301 cgttacccga aacactggct gaccgtgggc gatccggccc gtgaatttac catgacgcag
      361 tccgcaccgc tgatggtgct gccggatccg gatgagtttg tggtggtgca ggtgaaataa
      421 tccgtgagcg ggggcgaaat gcccccgtgt cttttttcac agggggctga tatggcaaca
      481 aaagaagaaa atcagaaaca tcttcgtcaa ctggctggcc tgctggggcg cgaggcggat
      541 atgtcgggga gtgctgcgga tattgcgcaa cgtgtgtctg agtgggaaga ggagcttgct
      601 gcttcccggg agggcattat gcctggtgat gagagcgggc ctgagcaaaa tcacacagac
      661 gatggtgagc agttgcacaa cactgatgct acggatgatg ttaaagcggt ccgtgtgcgg
      721 aaatgcctgc atgtgatggg gtattgcccg gagacaggcc gtcccgttga actgacgtac
      781 cggggcatgc gtgttctggt gccatcacca ctggcgacag ccatgataca gcacggaacg
      841 gctgagcatg cgtgattttc agaatgcctt tgatgctgcc cttgccgggg tggacagcac
      901 gattgttgaa gtgatggggc tctgtgcgca gttcacctcg ggggcacagt gtggcagcga
      961 agttcagggg gtttttgacg atccggagtc gctggggttt gccggtagcg gggtccgtat
     1021 tgaaggaagc tgcccgtcat tatttgtacg gacggatacg gttcgtgctg tgcggcgtgg
     1081 tgacacgctg accattaatg gtgagacatt ctgggtggat cgtgtttctc cggatgacgg
     1141 gggcagttgt tatctctggc tcaaccgtgg gcaaccaccg gcagttaacc ggcgacgata
     1201 aacgcagggt gaattatggc gataaaaggg cttgatcagg cgattgaaaa tctgagccgg
     1261 gttcgtaaaa acgccattcc ggcggcttca gcaatggcca ttaaccgcgt ggccacaacg
     1321 gcgattaatc agtcttcgtc acaggttgcc cgggagacca gggtgagccg gaaactggta
     1381 aaggaacggt ccagactgaa acgggccacg gtcagaaatc cgaatgccag aattatcgtt
     1441 aaccgcggtg atctcccggt gattaagctg gggatcagga tgccggggcg tcgtccggac
     1501 agcatactca aagccggtca gcatcggtat cagcgggcat ttattcagcg attaaaaaat
     1561 ggtcgctggc atgtcatgca gcgtgtggcc gggaaaaacc gttaccccat tgatgtggtg
     1621 aaaatcccga tggcggcccc actgaaacag gcgtttgatg agaatgttga ccgtatccgg
     1681 cgtgaacgcc tgcccggaga actggcatat gcgctgaaac aacaactgag gattgcgata
     1741 aaacgatgaa acatactgat atccgtgctg cagtgctgga tgcactggag ctgcatgaac
     1801 acggggcgac gctgtttgat ggtcgccccg ttgtttttga cgaagaggat tttcccgcgg
     1861 tcgcggttta tctgacggat gcagagtata ccggtgaaga gctggatgcg gatacctggc
     1921 gggccacact gcatattgaa gttttcctgc ctgctcaggt gccggattca gagctggatt
     1981 cgtggatgga gtcccggatt tatccggcga tgtccgcgat ccctgcactg gcagggatga
     2041 ttaccacgat ggttcagcag ggctatgact atcgtcgtga tgacgatatg gcgttatgga
     2101 gctctgcaga tttgacttat tccattacat acgagatgtg aggacgatat gccaacacca
     2161 aatcccctgg cgccggtaaa aggtgccggt accaccctgt gggtttataa cggtcagggt
     2221 gatgcttatg caaacccgtt gtcagatgat gactggcagc gactggctaa ggtgaaggat
     2281 ctgacgccgg gcgagatgac ggcagaatcc tacgatgata actacctgga tgatgaagac
     2341 gcggactgga gcgcgaccgg gcagggacag aaatctgcag gtgataccag ttttacactg
     2401 gcctggaaac cgggagaaga aggtcagaaa gggcttatag gctggtttga aagcggggat
     2461 gtgcgggcct ataaaatccg ttttccgaat ggcacggtgg atgtgtttcg tggctggatc
     2521 agcagtatcg gtaaggcagt gacggcgaaa gaagtgatca cccgaacagt gaaagtgacc
     2581 aacgtgggca aaccttccgt ggcggaagaa cgcagcgaaa ttacgccggc cactgcaatt
     2641 aaggtgacac cgacatccgg taccgtggaa aaaggaaaaa caaccaccct gactgtttct
     2701 tttgagccgg aaagtgcaac cgacaagacg ttcagagcgg tttccgccga tccgtcaacg
     2761 ggaaccattg ctgtgaaaga tatggtgatc actgtgacgg gggttaaggc tggaaaagtg
     2821 agtatccccg tgatttccgg taatggtcag tttgccacgg tagctgaagt caccgttact
     2881 gaagcgggcg ctgcagggta aacggaggtc atacatgttt ctgaaaacag aacaatttga
     2941 atataacggt gtgtctgtca cgctttccga attgtctgcg ctgcagcgta ttgagcatct
     3001 tgccctcctg aaacggcgtg cagaacaggc agaatccagc ggcaacctgc aggtaagcgt
     3061 ggaagatctc gtcagaaccg gcgcgtttct ggtggcgatg tccctgtggc ataaccatcc
     3121 gcagaaaacg gggtcaccgt caatgaatga ggccgtgatg cagattgagc aggaagtcct
     3181 gaccacctgg cctgctgatg ccattgcccg ggcggaagat gtggtgttgc gtctgtccgg
     3241 gatgagcggg cctgttcatg tggatacgga tattaccgaa gtggcgaaaa ataacgcgct
     3301 gactgatgat gatttttctg cgggaaagtc ttcgacggcg agctgaattt tgccctcaga
     3361 ctggcgcgtg agatggggag gcctgactgg cgcgccatgc ttgccgggat gacatccacc
     3421 gaatatgccg actggcgaca tttttaccgc atgcattatt ttca
//
LOCUS       DAZKHF020000339         3412 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2201S31241
            SAMN43267800-rid25106413.denovo.341, whole genome shotgun sequence.
ACCESSION   DAZKHF020000339 DAZKHF020000000
VERSION     DAZKHF020000339.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267800
            Sequence Read Archive: SRR30307263
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3412)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3412)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 3412)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350581.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350581.2
            Genome Coverage       :: 151x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:21:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,652
            CDSs (total)                      :: 5,557
            Genes (coding)                    :: 5,320
            CDSs (with protein)               :: 5,320
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 237
            CDSs (without protein)            :: 237
            Pseudo Genes (ambiguous residues) :: 0 of 237
            Pseudo Genes (frameshifted)       :: 102 of 237
            Pseudo Genes (incomplete)         :: 130 of 237
            Pseudo Genes (internal stop)      :: 52 of 237
            Pseudo Genes (multiple problems)  :: 41 of 237
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3412
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267800-rid25106413.denovo.341"
                     /serotype="O 26:H11"
                     /isolate="2201S31241"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-01"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            complement(7..141)
                     /locus_tag="ACHOSC_RS22615"
                     /old_locus_tag="pgaptmp_004523"
     CDS             complement(7..141)
                     /locus_tag="ACHOSC_RS22615"
                     /old_locus_tag="pgaptmp_004523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4550319.1"
                     /translation="MIPELIISGEEANCEFLMLVPGKMLFCSFCVMLMKQVRKIASVE
                     "
     gene            217..2778
                     /gene="mutS"
                     /locus_tag="ACHOSC_RS22620"
                     /old_locus_tag="pgaptmp_004524"
     CDS             217..2778
                     /gene="mutS"
                     /locus_tag="ACHOSC_RS22620"
                     /old_locus_tag="pgaptmp_004524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0030983 - mismatched DNA binding [Evidence
                     IEA];
                     GO_process: GO:0006298 - mismatch repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair protein MutS"
                     /protein_id="HGU4550320.1"
                     /translation="MSAIENFDAHTPMMQQYLRLKAQHPEILLFYRMGDFYELFYDDA
                     KRASQLLDISLTKRGASAGEPIPMAGIPYHAVENYLAKLVNQGESVAICEQIGDPATS
                     KGPVERKVVRIVTPGTISDEALLQERQDNLLAAIWQDSKGFGYATLDISSGRFRLSEP
                     ADRETMAAELQRTNPAELLYAEDFAEMSLIEGRRGLRRRPLWEFEIDTARQQLNLQFG
                     TRDLVGFGVENAPRGLCAAGCLLQYAKDTQRTTLPHIRSITMEREQDSIIMDAATRRN
                     LEITQNLAGGAENTLASVLDCTVTPMGSRMLKRWLHMPVRDTRVLLERQQTIGALQDF
                     TAELQPVLRQVGDLERILARLALRTARPRDLARMRHAFQQLPELRAQLETVDSAPVQA
                     LREKMGEFAELRDLLERAIIDTPPVLVRDGGVIASGYNEELDEWRALADGATDYLERL
                     EVRERERTGLDTLKVGFNAVHGYYIQISRGQSHLAPINYMRRQTLKNAERYIIPELKE
                     YEDKVLTSKGKALALEKQLYEELFDLLLPHLEALQQSASALAELDVLVNLAERAYTLN
                     YTCPTFIDKPGIRITEGRHPVVEQVLNEPFIANPLNLSPQRRMLIITGPNMGGKSTYM
                     RQTALIALMAYIGSYVPAQKVEIGPIDRIFTRVGAADDLASGRSTFMVEMTETANILH
                     NATEYSLVLMDEIGRGTSTYDGLSLAWACAENLANKIKALTLFATHYFELTQLPEKME
                     GVANVHLDALEHGDTIAFMHSVQDGAASKSYGLAVAALAGVPKEVIKRARQKLRELES
                     ISPNAAATQVDGTQMSLLSVPEETSPAVEALENLDPDSLTPRQALEWIYRLKSLV"
     gene            2884..>3412
                     /gene="pphB"
                     /locus_tag="ACHOSC_RS22625"
                     /old_locus_tag="pgaptmp_004525"
     CDS             2884..>3412
                     /gene="pphB"
                     /locus_tag="ACHOSC_RS22625"
                     /old_locus_tag="pgaptmp_004525"
                     /EC_number="3.1.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-serine/threonine phosphatase"
                     /protein_id="HGU4550321.1"
                     /translation="MPSTRYQKINTHHYRHIWVVGDIHGEYQLLQSRLHQLSFFPETD
                     LLISVGDNIDRGPESLDVLRLLNQPWFISVKGNHEAMALDAFATGDGNMWLASGGDWF
                     FDLNDSEQKEATDLLLKFHHLPHIIEITNDNIKYVIAHADYPGSEYLFGKEIAESELL
                     WPVDRVQKSLNGELQQ"
BASE COUNT          849 a          891 c          915 g          757 t
ORIGIN      
        1 ggaggtttat tcaaccgacg caattttgcg tacttgcttc ataagcatca cgcaaaagct
       61 gcaaaacagc atctttcccg gaaccagcat caagaactcg cagttcgctt cttcccctga
      121 aatgattaac tccggtatca tgtgcgcctt atgtgattac aacgaaaata aaaaccatca
      181 caccccattt aatatcaggg aaccggacat aaccccatga gtgcaataga aaatttcgac
      241 gcccatacgc ccatgatgca gcagtatctc aggctgaaag cccagcatcc cgagatcctg
      301 ctgttttacc ggatgggtga tttttatgaa ctgttttatg acgacgcaaa acgcgcgtcg
      361 caactgctgg atatttcact gaccaaacgc ggtgcttcgg cgggagagcc gatcccgatg
      421 gcggggattc cctaccatgc ggtggaaaac tatctcgcca aactggtgaa tcagggagag
      481 tccgttgcca tctgcgaaca aattggcgat ccggcgacca gcaaaggtcc ggttgagcgc
      541 aaagttgtgc gtatcgttac gccaggcacc atcagcgatg aagccctgtt acaggagcgt
      601 caggacaacc tgctggcggc tatctggcag gacagcaaag gtttcggcta cgcgacgctg
      661 gatatcagct ccgggcgttt tcgcctgagc gaaccggccg accgcgaaac gatggcggca
      721 gagctgcaac gcactaatcc ggcggaactg ctgtatgcag aagatttcgc cgagatgtcg
      781 ctgattgaag gccgtcgcgg cctgcgccgt cgcccgctgt gggagtttga aatcgacacc
      841 gcgcgccagc agttgaatct gcaatttggc acccgcgatc tggtcggttt tggcgtcgag
      901 aacgcgccgc gcggactttg tgctgccggt tgtctgttgc agtatgcgaa agatacccaa
      961 cgcacgaccc tgccgcatat tcgttctatc accatggaac gtgagcagga cagcatcatt
     1021 atggatgccg cgacacgtcg taacctggaa attactcaga acctggcggg cggtgcggaa
     1081 aatacgctgg cttctgtgct cgactgcacc gtcacgccga tgggtagtcg tatgctgaaa
     1141 cgctggctgc atatgccagt gcgcgatacc cgcgtgttgc ttgagcgcca gcaaactatt
     1201 ggcgcattgc aggatttcac cgccgagttg cagccggtac tgcgtcaggt cggcgacctg
     1261 gaacgtattc tggcacgtct ggctttacga actgctcgcc cacgcgatct ggcccgtatg
     1321 cgccacgctt tccagcaact gccggagctg cgtgcgcagt tagaaactgt cgatagtgca
     1381 ccggtacagg cgctacgtga gaagatgggc gagtttgccg agctgcgcga tctgctggag
     1441 cgagcaatca tcgacacacc gccagtgctg gtacgcgacg gtggtgttat cgcatcgggc
     1501 tataacgaag agctggatga gtggcgcgcg ctggctgacg gcgcgaccga ttatctggag
     1561 cgtctggaag tccgcgagcg tgaacgtacc ggcctggaca cgctgaaagt tggctttaat
     1621 gcggtgcacg gctactacat tcaaatcagc cgtgggcaaa gccatctggc acccatcaac
     1681 tacatgcgtc gccagacgct gaaaaacgcc gagcgctaca tcattccaga gctaaaagag
     1741 tacgaagata aagttctcac ctcaaaaggc aaagcactgg cactggaaaa acagctttat
     1801 gaagagctgt tcgacctgct gttgccgcat ctggaagcgt tgcaacagag cgcgagcgcg
     1861 ctggcggaac tcgacgtgct ggttaacctg gcggaacggg cctataccct gaactacacc
     1921 tgcccgacct tcattgataa accgggcatt cgcattaccg aaggtcgcca tccggtagtt
     1981 gaacaagtac tgaatgagcc atttatcgct aacccgctga atctgtcgcc gcagcgccgt
     2041 atgttgatta ttaccggccc gaacatgggc ggtaaaagta cctatatgcg ccagaccgca
     2101 ctgattgcgc tgatggccta catcggcagc tatgtaccgg cacaaaaagt cgagattgga
     2161 cctatcgatc gcatctttac ccgcgtaggc gcggcagatg acctggcttc cgggcgctca
     2221 acctttatgg tggagatgac tgaaaccgcc aatattttac ataacgccac cgaatacagt
     2281 ctggtgttaa tggatgagat cgggcgtgga acgtccacct acgatggtct gtcgctggcg
     2341 tgggcgtgcg cagaaaatct ggcgaataag attaaggcat tgacgctgtt tgccacccac
     2401 tatttcgagc tgacccagct accggagaaa atggaaggcg tcgccaacgt gcatctcgat
     2461 gcgctggagc acggagacac cattgccttt atgcatagcg tgcaggatgg cgcggcgagc
     2521 aaaagctacg gcctggcggt tgcagctctg gcaggcgtgc caaaagaggt gattaagcgc
     2581 gcacggcaaa aactgcgtga gctggaaagc atttcgccga acgccgccgc tacgcaagtg
     2641 gatggtacgc aaatgtcttt gctgtcagtg ccagaagaaa cttcgcctgc ggtcgaagca
     2701 ctggaaaatc tcgacccgga ttcactgact ccgcgtcagg cgctggagtg gatttatcgc
     2761 ttgaagagtc tggtgtaata acaattcccg atagtctttt gctatcggga atattaacga
     2821 caactgacga atcaaataaa aataccctgt ataataggaa agcttatttt acagggtaaa
     2881 accatgccat ctacacgcta tcaaaaaatc aatacccatc actatcgcca tatatgggtc
     2941 gttggtgata ttcatggtga atatcagtta ttacaatccc gcttacatca actctctttt
     3001 ttccccgaaa ccgacttact tatttctgtc ggcgataata ttgatcgtgg gccggagagt
     3061 cttgacgtcc tgcgcctgct aaaccagccc tggtttatct ccgttaaagg caaccacgaa
     3121 gcaatggcgc tggatgcgtt cgcgaccggc gatggcaata tgtggcttgc cagcggtggt
     3181 gactggtttt tcgatttaaa tgattcagag caaaaagaag ctacagatct gttgctgaaa
     3241 ttccatcacc ttccacatat tattgaaatc actaacgaca acataaaata tgtcatcgca
     3301 catgcagatt atccggggag tgaatatctc tttggtaaag aaatagcgga gagcgaatta
     3361 ctctggcctg ttgatcgtgt gcagaaatcg cttaatggcg agttacaaca aa
//
LOCUS       DAZKHF020000340         3383 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2201S31241
            SAMN43267800-rid25106413.denovo.342, whole genome shotgun sequence.
ACCESSION   DAZKHF020000340 DAZKHF020000000
VERSION     DAZKHF020000340.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267800
            Sequence Read Archive: SRR30307263
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3383)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3383)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 3383)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350581.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350581.2
            Genome Coverage       :: 151x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:21:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,652
            CDSs (total)                      :: 5,557
            Genes (coding)                    :: 5,320
            CDSs (with protein)               :: 5,320
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 237
            CDSs (without protein)            :: 237
            Pseudo Genes (ambiguous residues) :: 0 of 237
            Pseudo Genes (frameshifted)       :: 102 of 237
            Pseudo Genes (incomplete)         :: 130 of 237
            Pseudo Genes (internal stop)      :: 52 of 237
            Pseudo Genes (multiple problems)  :: 41 of 237
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3383
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267800-rid25106413.denovo.342"
                     /serotype="O 26:H11"
                     /isolate="2201S31241"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-01"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            complement(<1..151)
                     /locus_tag="ACHOSC_RS22630"
                     /old_locus_tag="pgaptmp_004526"
     CDS             complement(<1..151)
                     /locus_tag="ACHOSC_RS22630"
                     /old_locus_tag="pgaptmp_004526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide heptosyltransferase family
                     protein"
                     /protein_id="HGU4550322.1"
                     /translation="MFLVSLLRRIAFSYYDYKAYNFNIEKTDFVVIHIPDQIGDAMAI
                     FPVIRA"
     gene            466..1782
                     /gene="shiA"
                     /locus_tag="ACHOSC_RS22635"
                     /old_locus_tag="pgaptmp_004527"
     CDS             466..1782
                     /gene="shiA"
                     /locus_tag="ACHOSC_RS22635"
                     /old_locus_tag="pgaptmp_004527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate transporter"
                     /protein_id="HGU4550323.1"
                     /translation="MDSTLISTRPDEGTLSLSRARRAALGSFAGAVVDWYDFLLYGIT
                     AALVFNREFFPQVSPAMGTLAAFATFGVGFLFRPLGGVIFGHFGDRLGRKRMLMLTVW
                     MMGIATALIGILPSFSTIGWWAPILLVTLRAIQGFAVGGEWGGAALLSVESAPKNKKA
                     FYSSGVQVGYGVGLLLSTGLVSLISMMTTDVQFLSWGWRIPFLFSIVLVLGALWVRNG
                     MEESAEFEQQQHNQAAAKKRIPVIEALLRHPGAFLKIIALRLCELLTMYIVTAFALNY
                     STQNMGLPRELFLNIGLLVGGLSCLTIPCFARLADRFGRRRVYITGALIGMLSAFPFF
                     MALEAQSIFWIVFFSIMLANIAHDMVVCVQQPMFTEMFGASYRYSGAGVGYQVASVVG
                     GGFTPFIAAALITYFAGNWHSVAIYLLAGCLISAITALLMKDNQRA"
     gene            1884..3338
                     /gene="amn"
                     /locus_tag="ACHOSC_RS22640"
                     /old_locus_tag="pgaptmp_004528"
     CDS             1884..3338
                     /gene="amn"
                     /locus_tag="ACHOSC_RS22640"
                     /old_locus_tag="pgaptmp_004528"
                     /EC_number="3.2.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008714 - AMP nucleosidase activity
                     [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA];
                     GO_process: GO:0044209 - AMP salvage [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AMP nucleosidase"
                     /protein_id="HGU4550324.1"
                     /translation="MNNKGSGLTPAQALDKLDALYEQSVVALRNAIGNYITSGELPDE
                     NARKQGLFVYPSLTVTWDGSTTNPPKTRAFGRFTHAGSYTTTITRPTLFRSYLNEQLT
                     LLYQDYGAHISVQPSQHEIPYPYVIDGSELTLDRSMSAGLTRYFPTTELAQIGDETAD
                     GIYHPTEFSPLSHFDARRVDFSLARLRHYTGTPVEHFQPFVLFTNYTRYVDEFVRWGC
                     SQILDPDSPYIALSCAGGNWITAETEAPEEAISDLAWKKHQMPAWHLITADGQGITLV
                     NIGVGPSNAKTICDHLAVLRPDVWLMIGHCGGLRESQAIGDYVLAHAYLRDDHVLDAV
                     LPPDIPIPSIAEVQRALYDATKLVSGRPGEEVKQRLRTGTVVTTDDRNWELRYSASAL
                     RFNLSRAVAIDMESATIAAQGYRFRVPYGTLLCVSDKPLHGEIKLPGQANRFYEGAIS
                     EHLQIGIRAIDLLRAEGDRLHSRKLRTFNEPPFR"
BASE COUNT          794 a          820 c          808 g          961 t
ORIGIN      
        1 gcgcccgaat aacaggaaag atggccatag catcgccaat ctgatcggga atatgtatga
       61 caacaaagtc tgttttttca atattaaaat tataagcttt ataatcgtag taactaaatg
      121 caatacgtct caacaatgat actaaaaaca taactaacct cgcctcccta ctggttataa
      181 tgcaatgcag tctatcagaa tcatcagggt gcaattttgt gcatatgcgg acttttatgt
      241 ttcatatctc gaacctgtgg gccctctgct taatccttaa acaacaccag caactcctgc
      301 gcttttatct tccatcgaat ttttcatgtt gccgctaatc agccataaaa tcatttgcag
      361 atgcgctctg tcgaggtagt ctcataaggt tcgtttatag atcgacggca atgtgagtta
      421 ccttttccat actaattata aaaagacagt acaaacagga tcattatgga ctccacgctc
      481 atctccactc gtcccgatga agggacgctt tcgttaagtc gcgcccgacg agctgcgtta
      541 ggcagtttcg ctggtgccgt cgtcgactgg tatgattttt tactctatgg cattaccgcc
      601 gcactggtgt ttaatcgcga gtttttcccg caagtaagcc cggcgatggg aacgctcgcc
      661 gcatttgcca cctttggtgt cggcttcctt ttccgaccgc tcggcggtgt cattttcggt
      721 cactttggtg accggctggg acgtaagcgc atgttaatgc tgaccgtctg gatgatgggc
      781 atcgcgacag ccttgattgg tattcttcct tcattctcga ccattgggtg gtgggcacct
      841 attttgctgg tgacactgcg tgccattcag ggatttgccg tcggcggcga atggggaggc
      901 gcagcgttgc tttccgttga aagtgcgccg aaaaataaaa aagcctttta cagtagcggt
      961 gtacaagttg gctacggagt aggtttactg ctttcaaccg gactggtttc attgatcagt
     1021 atgatgacga ctgacgtaca gtttttaagc tggggctggc gcattccttt cctgtttagc
     1081 atcgtactgg ttctgggagc attgtgggtg cgcaatggca tggaggagtc cgcggaattt
     1141 gaacaacagc aacataatca agcggccgcg aaaaaacgca tcccggttat cgaagctctg
     1201 ttacgacatc ccggtgcttt cctgaagatt attgcgctac gactgtgcga gttgctgacg
     1261 atgtacatcg ttaccgcctt tgcacttaat tattcaaccc agaatatggg gttaccgcgc
     1321 gaacttttcc ttaatattgg tttgctggta ggtggattaa gctgcctgac aattccctgt
     1381 tttgccaggc ttgccgatcg ttttggtcgc cgcagggttt atatcacagg tgcgttaatc
     1441 ggaatgttga gcgcatttcc tttctttatg gcgcttgaag cacaatctat tttctggata
     1501 gttttcttct ccataatgct ggcaaacatt gcgcatgaca tggtggtgtg tgtgcaacaa
     1561 ccgatgttta ccgaaatgtt tggtgccagt tatcgctata gtggcgctgg agtcggttat
     1621 caggttgcca gtgtggttgg tggtggattt acacctttta ttgccgctgc actcatcact
     1681 tactttgccg ggaactggca tagcgtcgcc atttatttgc tggctggatg cctgatttcc
     1741 gcaataaccg ctttgttgat gaaagacaat caacgcgctt gatagcctgg cgaagatcat
     1801 ccgatcttcg ccttacactt ttgtttcaca ttcctgtgac atactatcgg atgtgcggta
     1861 attgtatgga acaggagaca cacatgaata ataagggctc cggtctgacc ccagctcagg
     1921 cactggataa actcgacgcg ctgtatgagc aatctgtagt tgctttacgc aacgccattg
     1981 gcaactatat tacaagtggc gaattacctg atgaaaacgc ccgcaaacaa ggtctttttg
     2041 tctatccatc actgaccgta acctgggacg gtagcacaac caatccccct aaaacgcgcg
     2101 catttggtcg ttttactcac gcaggcagct acaccaccac gattactcgc cctactctct
     2161 ttcgttcgta tctcaatgaa caacttacgt tgctgtatca ggattatggt gcgcatatct
     2221 cggtgcaacc ctcgcagcat gaaatccctt atccttatgt cattgatggc tctgaattga
     2281 cacttgatcg ctcaatgagc gctgggttaa ctcgctattt cccgacaaca gaactggcgc
     2341 aaattggcga tgaaactgca gacggcattt atcatccaac tgaattctcc ccgctatcgc
     2401 attttgatgc gcgccgcgtc gatttttccc tcgcgcggtt gcgccattat accggtacgc
     2461 cagttgaaca ttttcagccg ttcgtcttgt ttaccaacta cacacgttat gtggatgaat
     2521 tcgttcgttg gggatgcagc cagatcctcg atcctgatag tccctacatt gccctttctt
     2581 gtgctggcgg gaactggatc accgccgaaa ccgaagcgcc agaagaagcc atttccgacc
     2641 ttgcatggaa aaaacaccag atgcccgcgt ggcatttaat taccgccgat ggtcagggta
     2701 ttactctggt gaatattggc gtgggaccgt caaatgctaa aaccatctgc gatcatctgg
     2761 cagtactacg cccggatgtc tggttgatga ttggtcactg tggtggctta cgtgaaagcc
     2821 aggccattgg cgattatgta cttgcacatg cttatttacg cgatgatcac gttcttgatg
     2881 cggttctgcc gcccgatatt cctattccaa gcattgctga agtgcaacgt gcgctttatg
     2941 acgctaccaa gttagtcagc ggcaggcccg gtgaggaagt caaacagcgg ctgcgtacag
     3001 gtactgtggt aaccacagat gacaggaact gggaattacg ttactcagct tctgcacttc
     3061 gttttaactt aagccgggcc gtggcaattg atatggaaag tgcaaccatt gccgcgcaag
     3121 gatatcgttt ccgcgtgcca tacgggacac tactgtgtgt ttcagataaa ccgttgcatg
     3181 gcgagattaa acttcccggc caggctaacc gtttttatga aggcgctatt tccgaacacc
     3241 tgcaaattgg cattcgggca atcgatttgc tgcgcgcaga aggcgaccga ctgcattcac
     3301 gtaaattacg aacctttaat gagccgccgt tccgataata agttctgaac cttcttatca
     3361 aaaatagatg tgaaggatta agt
//
LOCUS       DAZKHF020000341         3370 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2201S31241
            SAMN43267800-rid25106413.denovo.343, whole genome shotgun sequence.
ACCESSION   DAZKHF020000341 DAZKHF020000000
VERSION     DAZKHF020000341.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267800
            Sequence Read Archive: SRR30307263
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3370)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3370)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 3370)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350581.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350581.2
            Genome Coverage       :: 151x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:21:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,652
            CDSs (total)                      :: 5,557
            Genes (coding)                    :: 5,320
            CDSs (with protein)               :: 5,320
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 237
            CDSs (without protein)            :: 237
            Pseudo Genes (ambiguous residues) :: 0 of 237
            Pseudo Genes (frameshifted)       :: 102 of 237
            Pseudo Genes (incomplete)         :: 130 of 237
            Pseudo Genes (internal stop)      :: 52 of 237
            Pseudo Genes (multiple problems)  :: 41 of 237
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3370
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267800-rid25106413.denovo.343"
                     /serotype="O 26:H11"
                     /isolate="2201S31241"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-01"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            complement(<1..1241)
                     /locus_tag="ACHOSC_RS22645"
                     /old_locus_tag="pgaptmp_004529"
     CDS             complement(<1..1241)
                     /locus_tag="ACHOSC_RS22645"
                     /old_locus_tag="pgaptmp_004529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease"
                     /protein_id="HGU4550325.1"
                     /translation="MSKVFICAAIPDEQAIKEEGAVAVATAIEAGDERRARAKFTWQF
                     LEQYPAAQDCAYKFLVCEDKPGMPRPAIDSWDTEYMQENRWDEESASFIPVEPESDPI
                     NVNFDKLSLEVQNAVLVKFGTCENITVDMAIDAQELLQEDVATFDGHIVEALMKTPEI
                     KAMYPERKLFAIGWVKHKCKPGTKWPEIQAELRNWKKRQDAERKETGKYTSVVDLARA
                     KANRQHTENPAEKIPPVTAAIHREYKQTWKTLDRELAYYLWPGDADAGNIDGSILRWA
                     KNEVIARDREDWKRISASMRKQPDALRYSRQTIFGLVRERPIDIHKDPVALNKYITEY
                     LTTKGVFEDEGRNQSATDTLSSPVPETDAVETAIPDNEKTECKVEVEPSVEREGPFYF
                     LFTDKDGEKYGRANKLSGLDKA"
     gene            complement(1335..1526)
                     /locus_tag="ACHOSC_RS22650"
                     /old_locus_tag="pgaptmp_004530"
     CDS             complement(1335..1526)
                     /locus_tag="ACHOSC_RS22650"
                     /old_locus_tag="pgaptmp_004530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1482 family protein"
                     /protein_id="HGU4550326.1"
                     /translation="MNTTFALVLTVYLVSGESLELVTGLYGSMKECMAAAAEQKIPGN
                     CYPVDKTTHTNNNEIPAGL"
     gene            complement(1523..1711)
                     /gene="dicB"
                     /locus_tag="ACHOSC_RS22655"
                     /old_locus_tag="pgaptmp_004531"
     CDS             complement(1523..1711)
                     /gene="dicB"
                     /locus_tag="ACHOSC_RS22655"
                     /old_locus_tag="pgaptmp_004531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division inhibition protein DicB"
                     /protein_id="HGU4550327.1"
                     /translation="MEKLSCNASTSELRFEIGVITGDKTFIEDAIKQRKLEQDLLNEV
                     CIPSMLARLDLLQKGYKQ"
     gene            2279..2488
                     /locus_tag="ACHOSC_RS22660"
                     /old_locus_tag="pgaptmp_004532"
     CDS             2279..2488
                     /locus_tag="ACHOSC_RS22660"
                     /old_locus_tag="pgaptmp_004532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4550328.1"
                     /translation="MPNKKRNPLIEKQIECLVNQLRQSGLLKTHSELRLTESAFDDKL
                     NNVLYNGIIDFNRSVGRRGPAGVSL"
     gene            complement(2489..3127)
                     /locus_tag="ACHOSC_RS22665"
                     /old_locus_tag="pgaptmp_004533"
     CDS             complement(2489..3127)
                     /locus_tag="ACHOSC_RS22665"
                     /old_locus_tag="pgaptmp_004533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4550329.1"
                     /translation="MEFKDLPPSIQEIAAHTLRHRLNELELESVTKKDTDNMARNVRD
                     AFTGLYFCASINKHDSESMANKIAETTAQNINTKPTEEEIDQFAHDAGLKNKKEKSPY
                     AGNMFVYDNLIRIRGEIPAEYLARVHQALLKNLETELFDGNTNGFFMVSGLEKDWDAE
                     KRWNVATWLFSNRAAALEASACICGLFLTDHKYNLDVYSYIYAEHGPLWIDW"
     gene            complement(3139..3291)
                     /locus_tag="ACHOSC_RS22670"
                     /old_locus_tag="pgaptmp_004534"
     CDS             complement(3139..3291)
                     /locus_tag="ACHOSC_RS22670"
                     /old_locus_tag="pgaptmp_004534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000379546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1391 family protein"
                     /protein_id="HGU4550330.1"
                     /translation="MDTIDLGNNESLVCGVFPNQDGTFTAMTYTKSKTFKTEAGARRW
                     LARNTD"
BASE COUNT          832 a          794 c          752 g          992 t
ORIGIN      
        1 gccttgtcca gaccagaaag tttgtttgcg cgaccgtatt tttcgccatc cttgtcggtg
       61 aagaggaagt agaacggccc ctcacgctct acagatggtt cgacttccac tttgcattcg
      121 gttttttcgt tgtccggaat tgccgtttcc actgcatcag tttctggtac tggcgacgag
      181 agagtatcag ttgcgctctg atttcttcct tcatcttcaa acacgccctt tgtagtcagg
      241 tattcagtaa tgtatttgtt cagtgccaca gggtctttgt gaatgtcgat cggacgttca
      301 cggacaaggc caaaaatagt ctggcggctg tagcgaagcg catcaggctg tttgcgcatt
      361 gatgcggaga tgcgcttcca gtcttcgcga tctctggcga taacttcatt tttagcccag
      421 cgaaggatgc tgccgtcaat gtttccggca tccgcatcac caggccagag atagtaggcc
      481 agctccctgt ccagggtttt ccatgtctgc ttgtattcgc gatgaatggc ggcagttaca
      541 ggagggattt tttctgctgg gttttcagtg tgctgtcggt tggctttggc gcgggcaaga
      601 tcaacaacag acgtgtattt tccagtctct ttgcgctctg cgtcctgccg ttttttccag
      661 ttacgtaatt cagcctgaat ttcgggccat ttggtacccg gcttacattt gtgtttaacc
      721 catccgatag cgaacagttt gcgttccgga tacatagctt taatttcagg cgttttcatc
      781 agtgcttcaa cgatatgccc gtcaaaggta gccacgtctt cttgcagtaa ttcctgcgcg
      841 tcaatcgcca tatcaacggt gatgttttca catgtaccga acttaaccag gaccgcgttc
      901 tgtacttcaa gggacagctt gtcaaaattg acgttaatag gatcggattc tggttcgacc
      961 ggaataaagg aagcggattc ctcatcccag cggttttcct gcatatattc ggtatcccag
     1021 gagtcgatag cagggcgggg catgccgggt ttatcttcgc agacaagaaa tttataagcg
     1081 cagtcctgag cagcaggata ttgctccagg aattgccagg taaatttggc acgggcgcgg
     1141 cgttcatcac cggcttcaat ggcagtggct acagcaactg cgccctcttc ttttattgcc
     1201 tgttcgtccg gaatggcggc gcaaataaag actttactca ttttgtttta acctcattac
     1261 agatttaagg gtgaacaaat ccctgccatt gctggcatat aaaaatgaaa ccggatatta
     1321 attacggtgc tgttttaaag tcctgccggt atttcgttat tattagtgtg agtagtttta
     1381 tctaccggat aacagttacc gggaattttt tgttctgctg ctgcagccat gcattctttc
     1441 attgaaccgt ataagccagt caccagctca agagattcgc cggaaacaag ataaactgtc
     1501 agaacgagtg caaatgttgt attcattgtt tatatccttt ttgcagcagg tccagacgag
     1561 ccagcattga aggaatgcat acttcattta acaggtcctg ctcgagtttt ctctgcttaa
     1621 tggcgtcttc aataaatgtt ttgtctccag tgataacgcc aatttcgaaa cgaagttcag
     1681 acgtgctggc attacatgat aacttttcca ttatcgcgtc ctcaacaatg aattttgtga
     1741 tgcggtgcct ggtgcctcca ggtgacgtta accagttaac aattaacgcc ggatacagag
     1801 aatccaccca taacactgtt tttggtttta actgttccgc gtgcgctgag ccgcattcac
     1861 cgcatcacaa aattcacttt taaaaaaggg cggcagagca gtcacggagt aaaactgata
     1921 ccgccaaatg tcaccagaat attgataaca gagggcgttg tagcgggggt gtcacttaag
     1981 cgtatggtca acctgacaac ccggtgtcct caactgggga aggaataacc ccgccatact
     2041 taccgccgcg ccatttcgcg gattgccaca accggaagcg cacgttcgaa gaaatctaac
     2101 gacaagcctt ctaagggaaa gagcttcgcc gtacgctttc gcgttatgcc ctgacttttc
     2161 agggaaatat cctttcagta aactgtcagt accggattct tatccgtgtc cggcgcacga
     2221 ccacacgtga cagcgtgttg gtctccattt ttaacccaga acctcaatgg aggataaaat
     2281 gccaaacaaa aaaagaaatc cgcttattga aaaacagatt gaatgcctgg taaatcaact
     2341 caggcaatca gggttattaa aaactcattc agagttgagg ctcacagaat cagcattcga
     2401 cgataaatta aataatgtcc tttataatgg cattattgat tttaatcgtt ctgttggtcg
     2461 ccgcggccct gctggtgttt ccttataatt accagtcaat ccagagtgga ccgtgttcag
     2521 cgtaaatata actgtacaca tccagattat atttgtggtc tgttaagaac aggccgcaaa
     2581 tacatgccga agcttccagt gcagcggctc tgttactgaa taaccatgta gcaacattcc
     2641 agcgtttttc tgcatcccag tctttctcaa ggcctgatac catgaagaaa ccgttagtgt
     2701 tgccatcaaa taattctgtt tccaaatttt taagcaatgc ctgatggact cttgccaggt
     2761 attccgccgg aatttcgcca cgaattctaa tgagattgtc ataaacaaac atgttccccg
     2821 catatggcga tttttctttc ttgtttttta aaccagcatc atgagcaaac tgatcaattt
     2881 cttcttccgt tggtttcgta ttgatgtttt gcgctgtcgt ttctgcaatt ttatttgcca
     2941 tactctctga gtcgtgttta tttatagacg cacagaaata caatccggta aacgcatcgc
     3001 gcacattacg agccatatta tcagtgtctt ttttcgttac cgattccaat tcaagttcgt
     3061 tcagacgatg acgaagtgtg tgtgctgcaa tctcctggat tgaaggaggt aaatctttaa
     3121 attccatcgt caacctcatc agtcagtgtt tctggctaac cagcgacgcg cgccagcttc
     3181 agttttaaac gttttgcttt tggtatacgt catcgcggtg aacgtaccgt cctggttggg
     3241 aaacacgccg cataccagag attcgttgtt gccaagatcg atagtatcca tgttgacctc
     3301 atttcccctt aacgccgggt agcggaacaa aaacctgctg catagttatt aaagttgaac
     3361 cctgccgtca
//
LOCUS       DAZKHF020000342         3346 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2201S31241
            SAMN43267800-rid25106413.denovo.344, whole genome shotgun sequence.
ACCESSION   DAZKHF020000342 DAZKHF020000000
VERSION     DAZKHF020000342.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267800
            Sequence Read Archive: SRR30307263
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3346)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3346)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 3346)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350581.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350581.2
            Genome Coverage       :: 151x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:21:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,652
            CDSs (total)                      :: 5,557
            Genes (coding)                    :: 5,320
            CDSs (with protein)               :: 5,320
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 237
            CDSs (without protein)            :: 237
            Pseudo Genes (ambiguous residues) :: 0 of 237
            Pseudo Genes (frameshifted)       :: 102 of 237
            Pseudo Genes (incomplete)         :: 130 of 237
            Pseudo Genes (internal stop)      :: 52 of 237
            Pseudo Genes (multiple problems)  :: 41 of 237
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3346
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267800-rid25106413.denovo.344"
                     /serotype="O 26:H11"
                     /isolate="2201S31241"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-01"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            complement(<1..485)
                     /locus_tag="ACHOSC_RS22675"
                     /old_locus_tag="pgaptmp_004535"
     CDS             complement(<1..485)
                     /locus_tag="ACHOSC_RS22675"
                     /old_locus_tag="pgaptmp_004535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000031374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ERF family protein"
                     /protein_id="HGU4550331.1"
                     /translation="MSKEFYARLAAIQENLNAPKNQYNSFGKYKYRSCEDILEGVKPL
                     LNGLFLSISDEVVLIGDRYYVKATATITDGENSHTATALAREEESKKGMDSAQVTGAT
                     SSYARKYCLNGLFGIDDAKDADTDEHKHQQNAAAKQSKPSPTPEQVLKAFTDAAMQKN
                     TV"
     gene            complement(587..745)
                     /locus_tag="ACHOSC_RS22680"
                     /old_locus_tag="pgaptmp_004536"
     CDS             complement(587..745)
                     /locus_tag="ACHOSC_RS22680"
                     /old_locus_tag="pgaptmp_004536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005106716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4550332.1"
                     /translation="MSFTDNWSDEEFIRQMKELIGNEGDIHVTCNHSEGEQVTEAHVH
                     AESSLVSP"
     gene            complement(742..894)
                     /gene="kil"
                     /locus_tag="ACHOSC_RS22685"
                     /old_locus_tag="pgaptmp_004537"
     CDS             complement(742..894)
                     /gene="kil"
                     /locus_tag="ACHOSC_RS22685"
                     /old_locus_tag="pgaptmp_004537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001243351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="host cell division inhibitory peptide Kil"
                     /protein_id="HGU4550333.1"
                     /translation="MRNEIAINHQMLRAAQNKAVIARFIGDSKMWLEANKAMKSAINL
                     PCYRRK"
     gene            complement(879..1010)
                     /locus_tag="ACHOSC_RS22690"
                     /old_locus_tag="pgaptmp_004538"
     CDS             complement(879..1010)
                     /locus_tag="ACHOSC_RS22690"
                     /old_locus_tag="pgaptmp_004538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000638547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protease FtsH-inhibitory lysogeny factor CIII"
                     /protein_id="HGU4550334.1"
                     /translation="MIYAIAGGARMGAFQLNESLLERITRKLRDGWKRVEVLLCAMK"
     gene            complement(1035..2003)
                     /locus_tag="ACHOSC_RS22695"
                     /old_locus_tag="pgaptmp_004539"
                     /pseudo
     CDS             complement(1035..2003)
                     /locus_tag="ACHOSC_RS22695"
                     /old_locus_tag="pgaptmp_004539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001351814.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="cell envelope biogenesis protein TolA"
     gene            2259..2798
                     /locus_tag="ACHOSC_RS22700"
                     /old_locus_tag="pgaptmp_004540"
     CDS             2259..2798
                     /locus_tag="ACHOSC_RS22700"
                     /old_locus_tag="pgaptmp_004540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000073667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="super-infection exclusion protein B"
                     /protein_id="HGU4550335.1"
                     /translation="MSNWWQELLRFFLRGLTLQQLIHMLIILIALIIITPASIKEWVD
                     IRNPEILPDHWMYYAMLLCISYVLNRGMEFIFLASSDRYKKYLSKRDEAKVIVETERL
                     FNSLSIQEKEVLAFAVMANNKIVLKHGDPVALSLMRKGLLHRSGVTYSASGKEKFVIP
                     DVWFHECYMRFAGKADELI"
     gene            complement(2822..3094)
                     /locus_tag="ACHOSC_RS22705"
                     /old_locus_tag="pgaptmp_004541"
     CDS             complement(2822..3094)
                     /locus_tag="ACHOSC_RS22705"
                     /old_locus_tag="pgaptmp_004541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antitermination protein N"
                     /protein_id="HGU4550336.1"
                     /translation="MSRKTEFKGTAASRRRARRANLQSQEAISSDKLHRPTPSRVVLQ
                     CKRKPAMRAEVITLTTLTRKYEGSTCLPNVAIYAAGYRKSKQLTAR"
     gene            complement(3072..3291)
                     /locus_tag="ACHOSC_RS22710"
                     /old_locus_tag="pgaptmp_004542"
                     /pseudo
     CDS             complement(3072..3291)
                     /locus_tag="ACHOSC_RS22710"
                     /old_locus_tag="pgaptmp_004542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502599.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT          736 a          776 c          709 g         1125 t
ORIGIN      
        1 acggtgtttt tctgcattgc tgcgtcagtg aatgctttta gaacctgttc aggtgtaggt
       61 gatggttttg attgctttgc tgctgcgttc tgctgatgtt tatgctcgtc ggtatctgca
      121 tctttcgcat catcaatgcc gaataaacca ttgaggcaat acttgcgtgc ataagagctt
      181 gtagctcccg taacttgtgc agaatccatt cctttcttgc tttcttcctc tcgtgcaaga
      241 gcggttgccg tatgactgtt ttcgccatcg gtaatagttg ccgtggcttt cacataatac
      301 cgatcaccaa tcaacacaac ttcatcgctg attgataaaa acaggccatt cagtaacggc
      361 ttaacgcctt caagaatatc ttcgcagctt ctgtatttat atttgccgaa tgagttgtac
      421 tgattctttg gcgcgttcag attctcctga atagctgcca gtcttgcgta aaattctttg
      481 ctcatatgat tgttctcaga atggacacgg cccaaggaaa taacgctgat ttaatacttc
      541 gactcgggac aaattaaggc atacccgcat tccttcgcgg tcaccattat ggcgatacca
      601 gagagctttc tgcgtgtaca tgcgcctctg taacttgctc tccttcactg tggttgcaag
      661 tgacatgaat atctccttcg ttaccgatta attctttcat ctgacgaatg aattcttcgt
      721 ctgaccagtt atctgtaaaa ctcatttcct gcgataacac ggaaggttga tagctgattt
      781 catcgcttta tttgcttcaa gccacatttt tgaatcacca ataaatcggg ctattactgc
      841 cttgttctgt gcagcacgaa gcatctggtg attgatggct atttcattgc gcataataag
      901 acctcaactc ttttccatcc gtcacgtaat ttacgggtga ttcgttcaag taaagattca
      961 tttaattgga aggcacccat gcgagcgcct cccgcgattg cgtaaatcat gggtggttcc
     1021 ttatgttggt tttattagta ggttattttt gttgcgaata attcgccttt tacgatggct
     1081 gttatgatat ttttagcaac atcttctgat gcgccaacct tgataaggtc agcaagtatt
     1141 ttgttattta cttctttccg gtgagcttta tcctttgctc tacgctcttc ttcgtccttg
     1201 attctttttt cttctgctat tctggcttgc tcttttgctt cagcctcgcg caggattcgt
     1261 tctgcctcct cctgtgcttt acggcgttct gcttcaattg ccgcatgctt ttctctttca
     1321 gctcgttctg ctgcctcttt tgcttcgcgc tgtgctcgtt gctcggcttc aatgcgttca
     1381 cgctctgcac gttcctctgc ggccttagct tctgcttctc gccttgctgc tgcttcaatt
     1441 tcggcttttg cctttgcttc ggcttcagct ctggctttct cttcagcttc tctttttaag
     1501 cgttcttcat gctctcgctt ttcctgctcc gctttgagtc ttgcctcttc tctttggcgg
     1561 tcaaattcgc gatccatcaa aatcgctatt tcatggtcag actcaatttg ctttgcgaga
     1621 gcttcagctg ctgccttagc ttcttcttcg gctttaatcc gcgcctgttc ttcctcataa
     1681 tcagtaagag gctggcgcgc cttggctttc agttcatcaa ggcgatcgcg cactgtcttg
     1741 cggttagcat caattagctt tggaatttcc ttcagttcag caacaaggtc tttgccaaga
     1801 ccatcgagat atgttttcgt ctgcgcaact ttatacgcca gataagcgat ctcctttctg
     1861 ccctttgccg ttgtgatatc aggcacaaag gacataactt cacgttcaac cttttgaaga
     1921 atttcttcaa tctggtcggc agactaaatt acagtcattg catttgcttt ttcaataaca
     1981 actaaatctg ttacttcact catatatcct ccgtcaaaaa aattgccctc acactggagg
     2041 gcaaagaaga tttccaataa tcagaacaag tcggctcctg tttagttacg agcgacattg
     2101 ctccgtgtat tcactcgttg gaatgaatac acagtgcagt gtttattcgt atgcctgtct
     2161 tttaaccaca tcaggctcgg tggttctcgt gtacccctac agcgagaaat cggataaact
     2221 ctattcaccc ctacagagag aatgatggag attcaccgat gagtaactgg tggcaggaac
     2281 tattacgttt cttcctgcgt ggtcttacgc tacaacagtt aattcatatg cttattattt
     2341 taattgcctt gataataatc accccagcat caattaaaga gtgggtagat ataaggaacc
     2401 ccgaaatact tccagatcac tggatgtatt acgcaatgct tttgtgtatc agttatgttc
     2461 tgaacagggg gatggagttt atatttctgg catcttcaga cagatataaa aaatatctca
     2521 gtaagagaga tgaggctaaa gtaattgtgg agactgagcg cctattcaat tctctgagta
     2581 ttcaggaaaa agaggtttta gcatttgctg ttatggcaaa taacaaaatc gtacttaagc
     2641 acggcgatcc ggtcgcttta tctcttatga gaaaaggcct tctccatcgc tcaggtgtga
     2701 cttacagcgc gtcaggtaaa gagaaatttg ttatacctga cgtctggttc catgagtgtt
     2761 atatgcgctt tgctggtaaa gctgatgagc taatttagtt cctctacggc gggggggatc
     2821 gtcacctcgc tgtcagttgt tttgatttcc ggtagcctgc cgcgtaaatg gctacgtttg
     2881 gaagacaagt tgaaccttca tattttctgg tcaacgttgt cagagttata acttctgctc
     2941 tcattgctgg tttgcgcttg cattgcaaga ccactcgtga aggggttggc ctgtgtagct
     3001 tgtcggagct aatcgcctcc tgactttgca ggtttgcgcg acgagctcta cggcgagaag
     3061 ctgcggtgcc tttaaattct gtttttctgg acatagattc ctcccgaata aactttggcg
     3121 atgcaatctc gaagctcctc ctgagacggt tacttcggca ttgcatccca cagcttatgt
     3181 ggttgggtga tctggctttt cagccacgta gtcgagtgtt cgacgttgtt taaagagcct
     3241 gccagtctgc tccgtttggc ttacagcgtc ctgctgatga gttgacttta tcgaaatgat
     3301 aaactgatgg caatagcaaa atgataaatt gtttgggttt tcaaat
//
LOCUS       DAZKHF020000343         3323 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2201S31241
            SAMN43267800-rid25106413.denovo.345, whole genome shotgun sequence.
ACCESSION   DAZKHF020000343 DAZKHF020000000
VERSION     DAZKHF020000343.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267800
            Sequence Read Archive: SRR30307263
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3323)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3323)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 3323)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350581.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350581.2
            Genome Coverage       :: 151x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:21:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,652
            CDSs (total)                      :: 5,557
            Genes (coding)                    :: 5,320
            CDSs (with protein)               :: 5,320
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 237
            CDSs (without protein)            :: 237
            Pseudo Genes (ambiguous residues) :: 0 of 237
            Pseudo Genes (frameshifted)       :: 102 of 237
            Pseudo Genes (incomplete)         :: 130 of 237
            Pseudo Genes (internal stop)      :: 52 of 237
            Pseudo Genes (multiple problems)  :: 41 of 237
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3323
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267800-rid25106413.denovo.345"
                     /serotype="O 26:H11"
                     /isolate="2201S31241"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-01"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            <1..281
                     /locus_tag="ACHOSC_RS22715"
                     /old_locus_tag="pgaptmp_004543"
     CDS             <1..281
                     /locus_tag="ACHOSC_RS22715"
                     /old_locus_tag="pgaptmp_004543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001439589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4550337.1"
                     /translation="DPRKAAVEAAIARAKARKLEQQQANAEPEEQVDPRKAAVEAAIA
                     RAKARKLEQQQTNAEPEEQVDPRKAAVAAAIARAQAKKAAQQKVVNED"
     gene            282..1340
                     /gene="rsxD"
                     /locus_tag="ACHOSC_RS22720"
                     /old_locus_tag="pgaptmp_004544"
     CDS             282..1340
                     /gene="rsxD"
                     /locus_tag="ACHOSC_RS22720"
                     /old_locus_tag="pgaptmp_004544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxD"
                     /protein_id="HGU4550338.1"
                     /translation="MVFRIASSPYTHNQRQTSRIMLLVLLAAVPGIAAQLWFFGWGTL
                     VQILLASVSALLAEALVLKLRKQSVAATLKDNSALLTGLLLAVSIPPLAPWWMVVLGT
                     VFAVIIAKQLYGGLGQNPFNPAMIGYVVLLISFPVQMTSWLPPHEIAVNIPGFIDAIQ
                     VIFSGHTASGGDMNTLRLGIDGISQATPLDTFKTSVRAGHSVEQIMQYPIYSGILAGA
                     GWQWVNLAWLAGGLWLLWQKAIRWHIPLSFLVTLALCATLGWLFSPETLAAPQIHLLS
                     GATMLGAFFILTDPVTASTTNRGRLIFGALAGLLVWLIRSFGGYPDGVAFAVLLANIT
                     VPLIDYYTRPRVYGHRKG"
     gene            1344..1964
                     /gene="rsxG"
                     /locus_tag="ACHOSC_RS22725"
                     /old_locus_tag="pgaptmp_004545"
     CDS             1344..1964
                     /gene="rsxG"
                     /locus_tag="ACHOSC_RS22725"
                     /old_locus_tag="pgaptmp_004545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxG"
                     /protein_id="HGU4550339.1"
                     /translation="MLKTIRKHGITLALFAAGSTGLTAAINQMTKTTIAEQASLQQKA
                     LFDQVLPAERYNNALAQSCYLVTAPELGKGEHRVYIAKQDDKPVAAVLEATAPDGYSG
                     AIQLLVGADFNGTVLGTRVTEHHETPGLGDKIELRLSDWITHFAGKKISGADDAHWAV
                     KKDGGDFDQFTGATITPRAVVNAVKRAGLYAQTLPAQLSQLPACGE"
     gene            1968..2663
                     /gene="rsxE"
                     /locus_tag="ACHOSC_RS22730"
                     /old_locus_tag="pgaptmp_004546"
     CDS             1968..2663
                     /gene="rsxE"
                     /locus_tag="ACHOSC_RS22730"
                     /old_locus_tag="pgaptmp_004546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxE"
                     /protein_id="HGU4550340.1"
                     /translation="MSEIKDVIVQGLWKNNSALVQLLGLCPLLAVTSTATNALGLGLA
                     TTLVLTLTNLTISTLRHWTPAEIRIPIYVMIIASVVSAVQMLINAYAFGLYQSLGIFI
                     PLIVTNCIVVGRAEAFAAKKGPALSALDGFSIGMGATCAMFVLGSLREIIGNGTLFDG
                     ADALLGSWAKVLRVEIFHTDSPFLLAMLPPGAFIGLGLMLAGKYLIDERMKKRRTEAA
                     AERALPNGETGNV"
     gene            2663..3298
                     /gene="nth"
                     /locus_tag="ACHOSC_RS22735"
                     /old_locus_tag="pgaptmp_004547"
     CDS             2663..3298
                     /gene="nth"
                     /locus_tag="ACHOSC_RS22735"
                     /old_locus_tag="pgaptmp_004547"
                     /EC_number="4.2.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003906 - DNA-(apurinic or apyrimidinic
                     site) endonuclease activity [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease III"
                     /protein_id="HGU4550341.1"
                     /translation="MNKAKRLEILTRLRENNPHPTTELNFSSPFELLIAVLLSAQATD
                     VSVNKATAKLYPVANTPAAMLELGVEGVKTYIKTIGLYNSKAENIIKTCRILLEQHNG
                     LVPEDRAALEALPGVGRKTANVVLNTAFGWPTIAVDTHIFRVCNRTQFAPGKNVEQVE
                     EKLLKVVPAEFKVDCHHWLILHGRYTCIARKPRCGSCIIEDLCEYKEKVDI"
BASE COUNT          784 a          856 c          888 g          795 t
ORIGIN      
        1 ttgatccgcg caaagccgcc gtcgaagccg ctattgcccg agccaaagcg cgcaaactgg
       61 aacagcaaca ggctaatgcg gaaccagaag aacaggttga tccgcgcaaa gccgccgtcg
      121 aagccgctat tgcccgtgcc aaagcgcgca agctggaaca gcaacagact aacgcagaac
      181 cagaagaaca ggttgatccg cgcaaagcgg cagttgccgc ggctattgcc cgcgctcagg
      241 ccaaaaaagc cgcccagcag aaggttgtaa acgaggacta aatggtattc agaatagcta
      301 gctcccctta tacccataac cagcgccaga catcgcgcat tatgctgttg gtgttgctcg
      361 cagccgtgcc aggaatcgca gcgcaactgt ggttttttgg ttggggtact ctcgttcaga
      421 tcctgttggc atcggttagt gctctgttag ccgaagctct cgtactcaaa ctacgcaagc
      481 agtcggtagc cgcaacgttg aaagataact cagcattgct gacaggctta ttgctggcgg
      541 taagtattcc ccccctcgcg ccatggtgga tggtcgtgct gggtacggtg tttgcggtga
      601 tcatcgctaa acagttgtat ggcggtctgg ggcaaaaccc gtttaatccg gcaatgattg
      661 gttatgtggt cttactgatc tccttcccgg tgcagatgac cagctggtta ccgccacatg
      721 aaattgcggt caacatccct ggttttatcg acgccataca ggttattttc agcggacata
      781 ccgccagtgg tggtgatatg aacacactac gcttaggtat tgatggcatt agtcaggcga
      841 caccgctgga tacatttaaa acctctgtcc gtgccggtca ttcggttgaa cagattatgc
      901 aatatccgat ctacagcggt attctggcgg gcgctggttg gcaatgggta aatctcgcct
      961 ggctggctgg cggcctgtgg ttgctatggc agaaagcgat tcgctggcat attcccctca
     1021 gcttcttagt aacgctggcg ttatgcgcaa cgttgggctg gttgttctca ccagaaacac
     1081 tggcagcacc gcaaattcat ctgctgtctg gtgcgaccat gctcggcgca ttctttattt
     1141 tgactgaccc ggttaccgct tctacgacca atcgtggtcg tcttattttc ggcgcgctgg
     1201 cgggcttatt agtctggttg atccgcagtt tcggcggcta tcctgacggc gtggcttttg
     1261 ccgtcctgct ggcgaacatc acggttcctc tgatcgatta ctacacgcgt ccgcgcgtct
     1321 acggccatcg caaagggtaa accatgctga aaactatccg aaaacacggc attacgttag
     1381 cgctatttgc agcgggttca acagggttaa ctgcggccat caaccagatg accaaaacga
     1441 cgattgctga acaggccagt ctgcaacaaa aggcattatt tgatcaggtg ctgccagccg
     1501 aacgctataa caatgcgctg gcacagagtt gctatctggt aactgcgcca gagttaggta
     1561 aaggtgagca tcgggtttac atcgccaaac aggatgacaa accggtagcc gccgttctgg
     1621 aagcaaccgc gccagatggc tattccggtg cgattcagct gctggtggga gccgatttta
     1681 acggcacggt acttggcacg cgcgtgacag agcaccacga aacgccaggg cttggcgata
     1741 aaatcgaact gcgcctttct gactggatca cccattttgc gggtaaaaaa atcagtggtg
     1801 cagatgatgc gcactgggcg gtgaagaaag atggtggtga tttcgaccag ttcaccggcg
     1861 cgacgattac tccccgcgcg gtggttaatg cggtaaaacg cgccggattg tacgctcaga
     1921 cgttaccggc acaactttct caacttcctg cctgtggaga ataaagcgtg agcgaaatta
     1981 aagacgttat tgttcagggg ttgtggaaaa acaactctgc gctggtccag ttgctcggcc
     2041 tttgtcctct gttggcggtc acgtccactg ccactaacgc tctgggttta ggacttgcga
     2101 ctacgctggt actgacgctg accaacctga ccatttcgac gctgcgtcac tggacgccag
     2161 ccgagatccg cattcccatt tacgtgatga tcatcgcctc ggtggtcagc gctgtacaga
     2221 tgctgatcaa cgcctacgcc tttggcctgt atcaatcatt agggattttt attccgctga
     2281 ttgtcactaa ctgtatcgtt gtgggccgcg ctgaagcctt cgccgccaaa aaaggtccgg
     2341 cgctttcggc actggacggc ttttcaattg gtatgggcgc aacctgcgcc atgttcgtgc
     2401 tgggttcact acgcgaaatt atcggcaatg gcacattgtt tgacggtgca gatgcgctgt
     2461 taggtagctg ggcaaaagta ttacgcgtgg aaattttcca caccgactcc cctttcctgc
     2521 tggcgatgct gcctccaggt gcatttattg gcctgggact gatgctggca ggaaaatacc
     2581 tgattgatga aagaatgaaa aagcgccgta ctgaagcagc tgccgaacgt gcattgccaa
     2641 acggtgaaac agggaatgtc tgatgaacaa agcaaaacgc ctagagatcc tcactcgcct
     2701 gcgtgagaac aatcctcatc ccaccaccga gcttaatttc agttcgcctt ttgaattgct
     2761 gattgccgta ctgctttccg ctcaggcgac cgatgtcagt gttaataagg cgacggcgaa
     2821 actctacccg gtggcgaata cgcctgcagc gatgcttgaa ctgggcgttg aaggggttaa
     2881 aacctatatc aaaacgattg ggctttataa cagcaaagca gaaaatatca tcaaaacctg
     2941 ccgtatctta ctggagcagc ataatggctt ggttccggaa gatcgtgctg cgcttgaagc
     3001 cctgcccggc gtaggtcgta aaacagccaa cgtcgtatta aacactgcat tcggctggcc
     3061 gactattgct gtcgacacgc acattttccg cgtttgtaat cgtactcaat ttgcgccggg
     3121 gaaaaatgtc gaacaggtag aagaaaagct actaaaagtg gttccggcag agtttaaagt
     3181 cgactgccac cattggttga tcctgcacgg gcgttatacc tgcattgccc gcaagccccg
     3241 ctgtggctct tgtattattg aagatctttg tgaatacaaa gagaaagttg acatctgaag
     3301 aaaaggggta acaccgatta ccc
//
LOCUS       DAZKHF020000344         3305 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2201S31241
            SAMN43267800-rid25106413.denovo.346, whole genome shotgun sequence.
ACCESSION   DAZKHF020000344 DAZKHF020000000
VERSION     DAZKHF020000344.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267800
            Sequence Read Archive: SRR30307263
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3305)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3305)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 3305)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350581.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350581.2
            Genome Coverage       :: 151x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:21:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,652
            CDSs (total)                      :: 5,557
            Genes (coding)                    :: 5,320
            CDSs (with protein)               :: 5,320
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 237
            CDSs (without protein)            :: 237
            Pseudo Genes (ambiguous residues) :: 0 of 237
            Pseudo Genes (frameshifted)       :: 102 of 237
            Pseudo Genes (incomplete)         :: 130 of 237
            Pseudo Genes (internal stop)      :: 52 of 237
            Pseudo Genes (multiple problems)  :: 41 of 237
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3305
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267800-rid25106413.denovo.346"
                     /serotype="O 26:H11"
                     /isolate="2201S31241"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-01"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            187..369
                     /locus_tag="ACHOSC_RS22740"
                     /old_locus_tag="pgaptmp_004548"
     CDS             187..369
                     /locus_tag="ACHOSC_RS22740"
                     /old_locus_tag="pgaptmp_004548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000870791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4550342.1"
                     /translation="MKWYTPEHVVEAFKKGELTRHQVRMNRNTARRCGYPEREKCFDD
                     ALKIIDELRKAEAEKE"
     gene            517..1182
                     /locus_tag="ACHOSC_RS22745"
                     /old_locus_tag="pgaptmp_004549"
     CDS             517..1182
                     /locus_tag="ACHOSC_RS22745"
                     /old_locus_tag="pgaptmp_004549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001706515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HGU4550343.1"
                     /translation="MNQNTKTIAEAKAQCLNKKDSKQTYVVVEQWLKMFKKWFSPSGK
                     EYTATLPAVEIYSYVAGFNEQGQECYANQKTLANIGMVSDRQVRNIINMFIDMGVLSV
                     EARDGQTSLYKALPFTDAHITPPEDVKTSVNVEQPAPETQEPLEATHSIEQETQAEIT
                     EQSDLFTWLISIDSQKPMQEETENFIKRALTSKRIILEDDAFNALMIALRKESEIYDS
                     IPF"
     gene            1195..1452
                     /locus_tag="ACHOSC_RS22750"
                     /old_locus_tag="pgaptmp_004550"
     CDS             1195..1452
                     /locus_tag="ACHOSC_RS22750"
                     /old_locus_tag="pgaptmp_004550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000876742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4550344.1"
                     /translation="MKYNLTLNKRTFEITESGYTLAKQLADSNYELPAIISFQELPHS
                     KEHTQHWLNHLSHFGVIKYEAVNSQTFRLLEISPFELWVAL"
     gene            1777..2157
                     /locus_tag="ACHOSC_RS22755"
                     /old_locus_tag="pgaptmp_004551"
     CDS             1777..2157
                     /locus_tag="ACHOSC_RS22755"
                     /old_locus_tag="pgaptmp_004551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000038144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4550345.1"
                     /translation="MSKKKGKKNVITTIGEACDQYALNNPRKYNYEHDAQPGEELIFI
                     LEEPSPELTDEELSLIQSMDGVYFNPRPRKTTVIDSSLLKRWQCLGFQDMHHFRGYMA
                     LNSLQYAGVRETELLLLDKIKIDD"
     gene            2199..2420
                     /locus_tag="ACHOSC_RS22760"
                     /old_locus_tag="pgaptmp_004552"
     CDS             2199..2420
                     /locus_tag="ACHOSC_RS22760"
                     /old_locus_tag="pgaptmp_004552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000608393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4550346.1"
                     /translation="MINKAILETTTFHDLKLLSECFISLIERAQEENDLGLLDDIIDN
                     IATYKIPLTIAMKKAREAQQQLEQSEEVA"
     gene            2420..2728
                     /locus_tag="ACHOSC_RS22765"
                     /old_locus_tag="pgaptmp_004553"
     CDS             2420..2728
                     /locus_tag="ACHOSC_RS22765"
                     /old_locus_tag="pgaptmp_004553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000005514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4550347.1"
                     /translation="MSEVHNVTIQMMDLVIDLQKAVNAALTFPSELTITQAVINLEST
                     REALQLLIAACEESEGAQIEADDLSNLIIVIHDTTEIMKEAVADLQPLINELIDKANN
                     "
     gene            2728..2937
                     /locus_tag="ACHOSC_RS22770"
                     /old_locus_tag="pgaptmp_004554"
     CDS             2728..2937
                     /locus_tag="ACHOSC_RS22770"
                     /old_locus_tag="pgaptmp_004554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000169008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4550348.1"
                     /translation="MTKTKRVRLLALTEEGKAGNCFTVGKVYTGLLDTYTRLMHVTDD
                     NGNDQTIVLDGNCVFGEWELTTEKS"
BASE COUNT         1159 a          619 c          669 g          858 t
ORIGIN      
        1 tataagaaat tgaaaatgat caagtttttt ataagagagg gttgacaaag ccgccgttat
       61 gtagtattgt aagtagggag gggagaaaca tctctctcca tctaaataat caaatcacag
      121 aacactgcac tttgagccgt caggctgcaa ggtgtgtttt tcttttatct aaataaggag
      181 ttttttatga aatggtatac accggaacac gtcgtagagg catttaaaaa aggtgagcta
      241 acacgacatc aagtaaggat gaacaggaac acagcgcggc gctgtggtta tccagagcgt
      301 gaaaaatgct ttgacgatgc actaaaaatt attgatgaac tgcgtaaggc agaagcagag
      361 aaagaataat aacaggagaa ggagaaaacc aaatgaatat taattttgat tctgcaactt
      421 ataacgcgtt gttaaatcgt gcaaaggcag aagggaaatc aattccaaaa cttgttaatg
      481 aattactaat tacagtcctt acaaaggagg aactaaatga accaaaacac caaaacaatt
      541 gccgaagcaa aagcgcaatg cctcaataag aaagatagta aacaaacata tgtagtagtt
      601 gaacaatggc tgaagatgtt taaaaagtgg ttctcaccgt caggtaagga atacaccgcg
      661 acacttccag ccgttgaaat ctacagttac gtagctgggt tcaatgaaca agggcaagaa
      721 tgttacgcca atcaaaaaac attagctaat attggaatgg ttagtgatcg tcaggtgcgt
      781 aatattataa atatgtttat tgatatgggt gttctttctg tagaggcacg ggacgggcaa
      841 acatcactct ataaagcatt accctttaca gatgcacaca tcacaccacc ggaagacgta
      901 aaaacctctg taaacgtaga acagcctgca cctgaaacac aggaaccgtt agaagcgaca
      961 cactctattg agcaggaaac acaggcagaa atcacggaac aatctgatct ttttacatgg
     1021 cttatttcca tagacagcca aaagcctatg caagaagaaa cagaaaattt tataaaacgt
     1081 gcactgacct cgaagaggat aattcttgaa gatgatgctt ttaatgcact gatgatagcc
     1141 ctgagaaaag aaagtgaaat ctatgactca ataccatttt aaggagaata aaaaatgaaa
     1201 tacaacctga cattaaacaa acgaacattc gaaattacag aaagcggcta cacactggca
     1261 aaacagcttg ctgatagcaa ttatgagcta cccgcgatta tcagctttca ggagttacct
     1321 catagtaaag aacacacaca gcactggtta aatcacctga gccattttgg ggtaattaag
     1381 tatgaggctg tgaatagtca gacgttccgt ttgcttgaga taagcccttt cgaattgtgg
     1441 gtggcgctgt gaccatattc gatagcgaat tttcacaaga aacctttctg caatatttac
     1501 gggaaagaac gggaatgatt ctgtttgatt ctgagttttc cggtgtggta ggtgagcaat
     1561 ttttatatgc tcttctcgat agcctaagta aacaaggatt attgcagtat accaaaactc
     1621 atatgaaagc attcagaatc gactctgtag cttaaaacaa acaactatca aacatcttga
     1681 agggtgaaac aatggaaagc ttaatatttt tcttagtatt tatgatcgct tcctttttta
     1741 acactgcaac aagaaaaaca aaacgaaggg gaagcaatga gcaagaaaaa aggcaagaaa
     1801 aacgtaataa caactattgg cgaagcatgt gatcagtatg cgttaaacaa tcctcgcaaa
     1861 tataattatg agcatgatgc acaaccaggc gaggaattga tctttatact tgaagaacca
     1921 agcccagaat taacagacga agaattatca ctcattcaaa gtatggacgg tgtttacttt
     1981 aatccgcgcc cacgcaaaac aacggttata gattccagtc ttctgaaaag atggcagtgt
     2041 ttgggttttc aggatatgca ccactttagg gggtatatgg ctcttaacag tctgcaatat
     2101 gcaggggtga gagaaaccga attacttctg cttgataaaa taaaaatcga cgattaacag
     2161 cgtttgctga atataaccct taacaaaagg aaaataaaat gattaacaaa gcaattttag
     2221 agacaaccac ttttcacgat cttaaacttc tttctgaatg cttcataagc ctgattgaac
     2281 gtgcacagga agaaaacgat ctcggtttac tcgatgatat tatagacaac attgcaactt
     2341 ataaaatccc gctcactatt gctatgaaga aagctcgtga agcacaacaa caattagaac
     2401 aatcagagga agtggcataa tgagcgaagt acacaacgta actattcaaa tgatggatct
     2461 ggttattgat ttacagaaag cagtaaacgc ggctctgact tttccaagcg agctaaccat
     2521 cacacaggcg gttattaatc tggaatcgac gcgggaagca ctgcaactac ttattgcagc
     2581 gtgcgaagag agcgaaggtg cacagattga agctgatgac ctttctaatc tgatcatcgt
     2641 cattcatgac accactgaaa tcatgaaaga agcggtagca gatctgcaac ccctcattaa
     2701 tgaattaatc gataaggcta acaactaatg acaaaaacta aacgtgtgcg ccttctggcg
     2761 ctcacagaag aagggaaagc gggtaattgt ttcacagtag gtaaagtgta taccggctta
     2821 ctcgacactt acacccgcct gatgcacgta accgatgata acggtaacga tcaaacaatc
     2881 gttttagatg gtaattgcgt atttggtgag tgggaactga caaccgaaaa aagttgaaaa
     2941 taattgcatt tacctattga cttttactaa caaaaatgtt agaataataa atgtggggtt
     3001 ttgaatcgaa aatatgtgtt agctggcgtt aatccttctc ccgattctaa tcctacacga
     3061 aagcggggta gacctcaagg gggcgcgccc tatctaacct atcccgcttt ccctttacag
     3121 tgatttttta gaaattgttt ccctcgttaa tagtttcatt ttattttcct tattgcttta
     3181 tcaaaattgt gtcttaagaa tgcaggggct acggatttgg cccctcatct ttttaaccaa
     3241 ctgcgaagca tgaaacactt tctaaaaaca ctgtttacag tgtctataaa ccccgtataa
     3301 acaaa
//
LOCUS       DAZKHF020000345         3305 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2201S31241
            SAMN43267800-rid25106413.denovo.347, whole genome shotgun sequence.
ACCESSION   DAZKHF020000345 DAZKHF020000000
VERSION     DAZKHF020000345.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267800
            Sequence Read Archive: SRR30307263
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3305)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3305)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 3305)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350581.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350581.2
            Genome Coverage       :: 151x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:21:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,652
            CDSs (total)                      :: 5,557
            Genes (coding)                    :: 5,320
            CDSs (with protein)               :: 5,320
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 237
            CDSs (without protein)            :: 237
            Pseudo Genes (ambiguous residues) :: 0 of 237
            Pseudo Genes (frameshifted)       :: 102 of 237
            Pseudo Genes (incomplete)         :: 130 of 237
            Pseudo Genes (internal stop)      :: 52 of 237
            Pseudo Genes (multiple problems)  :: 41 of 237
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3305
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267800-rid25106413.denovo.347"
                     /serotype="O 26:H11"
                     /isolate="2201S31241"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-01"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            169..1689
                     /locus_tag="ACHOSC_RS22775"
                     /old_locus_tag="pgaptmp_004555"
     CDS             169..1689
                     /locus_tag="ACHOSC_RS22775"
                     /old_locus_tag="pgaptmp_004555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418214.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YifB family Mg chelatase-like AAA ATPase"
                     /protein_id="HGU4550349.1"
                     /translation="MSLSIVHTRAALGVNAPPITVEVHISKGLPGLTMVGLPETTVKE
                     ARDRVRSAIINSGYEYPAKKITINLAPADLPKEGGRYDLPIAIALLAASEQLTANKLD
                     EYELVGELALTGALRGVPGAISSATEAIKSGRKIIVAKDNEDEVGLINGEGCLIADHL
                     QAVCAFLEGKHALERPKPTDAVSRALQHDLSDVVGQEQGKRGLEITAAGGHNLLLIGP
                     PGTGKTMLASRINGLLPDLSNEEALESAAILSLVNAESVQKQWRQRPFRSPHHSASLT
                     AMVGGGAIPGPGEISLAHNGVLFLDELPEFERRTLDALREPIESGQIHLSRTRAKITY
                     PARFQLVAAMNPSPTGHYQGNHNRCTPEQTLRYLNRLSGPFLDRFDLSLEIPLPPPGI
                     LSKTVVPGESSATVKQRVMAARERQFKRQNKLNAWLDSPEIRQFCKLESEDAMWLEGT
                     LIHLGLSIRAWQRLLKVARTIADIDQSDIITRQHLQEAVSYRAIDRLLIHLQKLLT"
     gene            complement(1714..2052)
                     /gene="maoP"
                     /locus_tag="ACHOSC_RS22780"
                     /old_locus_tag="pgaptmp_004556"
     CDS             complement(1714..2052)
                     /gene="maoP"
                     /locus_tag="ACHOSC_RS22780"
                     /old_locus_tag="pgaptmp_004556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="macrodomain Ori organization protein MaoP"
                     /protein_id="HGU4550350.1"
                     /translation="MAESFTTTNRYFDNKHYPRGFSRHGDFTIKEAQLLERHGYAFNE
                     LDLGKREPVTEEEKLFVAVCRGEREPVTEAERVWSKYMTRIKRPKRFHTLSGGKPQVE
                     GAEDYTDSDD"
     gene            2171..3010
                     /gene="hdfR"
                     /locus_tag="ACHOSC_RS22785"
                     /old_locus_tag="pgaptmp_004557"
     CDS             2171..3010
                     /gene="hdfR"
                     /locus_tag="ACHOSC_RS22785"
                     /old_locus_tag="pgaptmp_004557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator HdfR"
                     /protein_id="HGU4550351.1"
                     /translation="MDTELLKTFLEVSRTRHFGRAAESLYLTQSAVSFRIRQLENQLG
                     VNLFTRHRNNIRLTAAGEKLLPYAETLMSTWQAARKEVAHTSRHNEFSIGASASLWEC
                     MLNQWLGRLYQNQDAHTGLQFEARIAQRQSLVKQLHERQLDLLITTEAPKMDEFSSQL
                     LGYFTLALYTSAPSKLKGDLNYLRLEWGPDFQQHEAGLIGADEVPILTTSSAELAQQQ
                     IAMLNGCTWLPVSWARKKGGLHTVVDSTTLSRPLYAIWLQNSDKNTLIRDLLKINVLD
                     EVY"
     gene            complement(3106..3181)
                     /locus_tag="ACHOSC_RS22790"
                     /old_locus_tag="pgaptmp_004558"
     tRNA            complement(3106..3181)
                     /locus_tag="ACHOSC_RS22790"
                     /old_locus_tag="pgaptmp_004558"
                     /product="tRNA-Trp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(3146..3148),aa:Trp,seq:cca)
     gene            complement(3190..3266)
                     /locus_tag="ACHOSC_RS22795"
                     /old_locus_tag="pgaptmp_004559"
     tRNA            complement(3190..3266)
                     /locus_tag="ACHOSC_RS22795"
                     /old_locus_tag="pgaptmp_004559"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(3230..3232),aa:Asp,seq:gtc)
BASE COUNT          883 a          817 c          812 g          793 t
ORIGIN      
        1 tcctcgtagt tcatcctttg tccgctgcat ttttgcgagc atcattccac cgccagaaca
       61 actcaacttc acttgcacaa ataaacagga atcagccact aaaattcacg aattagcggg
      121 ctacagcagc gatatcttat gcacataata gtgactcaag gagggcttat gtcactgtca
      181 attgttcata cccgcgcagc cctgggagta aatgcgcccc caatcactgt tgaggtacat
      241 atcagtaaag gtctacccgg cttaacgatg gtgggcttac cagaaacaac tgtaaaagaa
      301 gctcgcgatc gcgtgcgcag cgccattatc aatagcggat atgaatatcc ggcgaaaaaa
      361 atcaccatca acctggctcc agccgatctg ccaaaagaag ggggacgata tgatttacct
      421 atcgccattg cgttgctggc ggcctcagaa cagcttacag ccaataagtt agatgaatat
      481 gaattagtcg gagaactggc gcttacaggc gctctgcgtg gcgttcccgg cgcaatctcc
      541 agtgcaactg aagctattaa gtcgggcaga aaaattatcg tcgcgaaaga taacgaagat
      601 gaagtggggc taattaacgg tgaaggatgc ctgatagccg atcatctgca ggctgtctgt
      661 gcgtttctgg aaggtaagca cgctctcgaa cgcccgaaac caactgatgc agtatcccgg
      721 gcgctacaac atgatctcag tgatgttgtc ggtcaggagc aaggaaagcg aggactggaa
      781 attaccgccg ctggcgggca caacctttta ctgattgggc cgccgggaac aggtaaaaca
      841 atgctcgcca gccgtattaa tggcctgttg ccagatttaa gcaatgaaga ggcactggag
      901 agcgctgcga tattaagtct ggtaaatgct gaatcagtac aaaaacaatg gcggcagcgc
      961 ccgttccgct caccgcatca cagtgcgtcg ttaactgcga tggtgggcgg tggtgcaatt
     1021 ccagggccag gtgaaatttc gttggcgcat aacggcgtgc tttttcttga tgagctacct
     1081 gaatttgaac ggcgtacact ggatgccttg cgagagccga ttgaatccgg gcagatccat
     1141 ctttcacgca cacgagccaa aataacctat ccagcccgtt tccagcttgt tgcggcgatg
     1201 aatcccagcc ctaccggaca ttatcaggga aaccataacc gctgcacgcc agaacagaca
     1261 ttacgttatc tcaaccggct ctcggggccc tttctcgacc gcttcgatct ctcactggag
     1321 atcccattac caccccccgg cattttgagt aaaacggtag tgccgggaga aagcagcgcc
     1381 accgttaaac aacgcgtaat ggccgccaga gagcgccaat ttaagcggca gaataaactg
     1441 aatgcctggc tggatagtcc ggaaatacgc caattctgca aacttgagag cgaagatgcg
     1501 atgtggttgg aaggaacact gatccatctg gggttatcga ttcgtgcctg gcagcggtta
     1561 ttgaaagttg cacgaaccat tgctgatatt gatcagtctg acattatcac acgtcagcat
     1621 ttgcaggagg cagttagcta tcgtgcgatt gaccgtttgc tcatccatct gcaaaaacta
     1681 ctgacataaa aaaagggcat ttcgcccttt ttattaatca tcagaatcgg tgtagtcttc
     1741 agcaccttca acctgcggtt taccgccgga aagggtgtga aaacgttttg gacgcttaat
     1801 acgcgtcata tacttggacc acacgcgttc tgcttctgtc actggctcac gttcgccacg
     1861 gcatactgct acgaagagtt tctcttcctc ggtaaccggc tcgcgtttgc caagatccaa
     1921 ctcattgaag gcataaccat gacgctcaag cagttgtgcc tctttgatgg tgaaatcacc
     1981 atgacgagag aatccacgtg gataatgttt attgtcgaaa tatcgattag tcgtcgtaaa
     2041 gctttccgcc atcctgcacg ctcctaattc tttgaccgag ctagttatgg cgcggagtat
     2101 tagttacgct tgacagagtg taaaacaaaa catttaaatc ataacgacaa ataattttgc
     2161 ggagagcact gtggatacgg aattgttaaa aactttcctg gaagttagcc gaacgcgtca
     2221 ctttggtcga gcggctgaat cgctctatct gacccagtca gcagtgagct ttcgaatcag
     2281 acaactggaa aatcaactgg gtgtgaacct tttcacccgc cacagaaaca atatccgttt
     2341 aaccgctgcc ggtgaaaaac tactgcctta tgcagaaacg ctcatgagca cctggcaggc
     2401 cgcccgcaag gaggtggcgc atacctcacg acataacgag ttttctatcg gtgccagcgc
     2461 ctcattgtgg gaatgtatgc ttaatcagtg gctgggacgt ttgtatcaaa atcaggatgc
     2521 ccatacaggc ttacagttcg aagcgcgaat tgcccaacgg cagtctctgg taaaacagct
     2581 gcatgaacgc cagcttgatc ttcttattac cactgaagcg cccaaaatgg acgaatttag
     2641 tagtcagttg ctgggatatt tcactttagc gctttatacc agtgcccctt caaaactaaa
     2701 gggagatctt aattatctgc gacttgagtg ggggccagat tttcaacagc atgaggcagg
     2761 tttgatcggt gctgacgaag tgcccattct gacaaccagt tctgctgaac tggcacagca
     2821 acagattgcg atgcttaatg gttgcacctg gctacccgtc agctgggcgc gtaaaaaagg
     2881 cggcctgcat accgttgtcg atagcacaac actttcacgg ccgctttatg ccatatggct
     2941 gcaaaatagc gataaaaata cgttgattcg cgatcttttg aaaattaacg tgctggatga
     3001 agtgtattaa tatgaatggc tggcaaggat gccggtagaa ggatttactt cggagagggt
     3061 tatttcagat aaaaaaaatc cttagctttc gctaaggatg atttctggca ggggcggaga
     3121 gactcgaact cccaacaccc ggttttggag accggtgctc taccaattga actacgcccc
     3181 taattagggt ggcggaacgg acgggactcg aacccgcgac cccctgcgtg acaggcaggt
     3241 attctaaccg actgaactac cgctccaccg aattctttta caaccaccgg ttttatgacc
     3301 ggctt
//
LOCUS       DAZKHF020000346         3303 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2201S31241
            SAMN43267800-rid25106413.denovo.348, whole genome shotgun sequence.
ACCESSION   DAZKHF020000346 DAZKHF020000000
VERSION     DAZKHF020000346.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267800
            Sequence Read Archive: SRR30307263
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3303)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3303)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 3303)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350581.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350581.2
            Genome Coverage       :: 151x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:21:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,652
            CDSs (total)                      :: 5,557
            Genes (coding)                    :: 5,320
            CDSs (with protein)               :: 5,320
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 237
            CDSs (without protein)            :: 237
            Pseudo Genes (ambiguous residues) :: 0 of 237
            Pseudo Genes (frameshifted)       :: 102 of 237
            Pseudo Genes (incomplete)         :: 130 of 237
            Pseudo Genes (internal stop)      :: 52 of 237
            Pseudo Genes (multiple problems)  :: 41 of 237
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3303
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267800-rid25106413.denovo.348"
                     /serotype="O 26:H11"
                     /isolate="2201S31241"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-01"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            23..538
                     /gene="ehxC"
                     /locus_tag="ACHOSC_RS22800"
                     /old_locus_tag="pgaptmp_004560"
                     /gene_synonym="hlyC"
     CDS             23..538
                     /gene="ehxC"
                     /locus_tag="ACHOSC_RS22800"
                     /old_locus_tag="pgaptmp_004560"
                     /gene_synonym="hlyC"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000839950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enterohemolysin-activating
                     lysine-acyltransferase EhxC"
                     /protein_id="HGU4550352.1"
                     /translation="MKSNAFDVMGKVAWLWACSPLHKKWPLSVFAINVIPAIQTNQFA
                     LLIKDELPVAFCSWASLDLECEVKYINDVTSLYAKDWMSGERKWFIDWIAPFGHNMEL
                     YKYMRKKYPYELFRAIRLDESSKTGKIAEFHGGGIDKKLASKIFRQYHHELMSEVKNR
                     QDFNFNIEKEN"
     gene            540..>3303
                     /gene="ehxA"
                     /locus_tag="ACHOSC_RS22805"
                     /old_locus_tag="pgaptmp_004561"
     CDS             540..>3303
                     /gene="ehxA"
                     /locus_tag="ACHOSC_RS22805"
                     /old_locus_tag="pgaptmp_004561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enterohemolysin EhxA"
                     /protein_id="HGU4550353.1"
                     /translation="MTVNKIKNIFNNATLTTKSAFNTASSSVRSAGKKLILLIPDNYE
                     AQGVGINELVKAADELGIEIHRTERDDTAIANQFFGAAEKVVGLTERGVAIFAPQLDK
                     LLQKYQKVGSKIGRTAENVGNNLGKAGTVLSALQNFTGIALSGMALDELLRKQREGED
                     ISQNDIAKSSIELINQLVDTVSSINSTVDSFSEQLNQLGSFLSSKPRLSSVGGKLQNL
                     PDLGSLGDGLDVVSGILSAVSASFILGNSDAHTGTKAAAGIELTTQVLGNVGKAVSQY
                     ILAQRMAQGLSTTAASAGLITSAVMLAISPLSFLAAADKFERAKQLESYSERFKKLNY
                     EGDALLAAFHKETGAIDAALTTINTVLSSVSAGVSAASSASLIGAPISMLVSALTGTI
                     SGILEASKQAMFEHVAEKFAARINEWEKEHGKNYFENGYDARHAAFLEDSLSLLADFS
                     RQHAVERAVAITQQHWDEKIGELAGITRNADRSQSGKAYINYLENGGLLEAQPKEFTQ
                     QVFDPQKGTIDLSTGNVSSVLTFITPTFTPGEEVRERKQSGKYEYMTSLIVNGKDTWS
                     VKGIKNHKGVYDYSKLIQFVEKNNKHYQARMISELGDKDDVVYSGAGSSEVFAGEGHD
                     TVSYNKTDVGKLTIDATGASKPGEYIVSKNMYGDVKVLQEVVKEQEVSVGKRTEKIQY
                     RDFEFRTGGIPYDVIDNLHSVEELIGGKHDDEFKGGKFNDIFHGADGNDYIEGNYGND
                     RLYGDDGDDYISGGQGDDQLFGGSGNDKLSGGDGNNYLTGGSGNDELQAHGAYNILSG
                     GTGDDKLYGGGGIDLLDGGEGNDYLNGGFGNDIYVYRQNYGHHTIADEGGKGDRLHLS
                     DISFDDIAFKRVGNDLIMNKAINGVLSFNESNDVNGITFKNWFAKDASGADNHLVEVI
                     TDKDGRE"
BASE COUNT         1082 a          501 c          787 g          933 t
ORIGIN      
        1 ttttcttatt tatttaaaca ttatgaaatc taatgctttt gacgtcatgg ggaaggttgc
       61 atggctatgg gcctgttctc ctctgcataa aaaatggcca ttatctgttt ttgctattaa
      121 tgtcatacct gctattcaga caaatcaatt tgctctctta ataaaggatg agcttcctgt
      181 agctttttgt agctgggcca gtttagatct ggaatgtgag gtaaaatata taaatgatgt
      241 aacatctttg tatgcaaaag actggatgtc gggagaaaga aagtggttta ttgactggat
      301 agctccattt ggacacaaca tggagttata taaatacatg aggaaaaagt atccttatga
      361 gttattcaga gcaataaggt tagatgagag cagtaaaaca ggaaaaatag ctgaatttca
      421 tggagggggg atagataaaa aactggcatc aaaaatattc aggcaatacc atcatgaact
      481 tatgtctgaa gtaaaaaaca gacaagattt taattttaat attgagaaag aaaactaata
      541 tgacagtaaa taaaataaag aacattttca ataatgcgac attgactaca aaatcagcat
      601 ttaatacagc atcatcaagc gtacgttccg ctggcaaaaa actcatatta ttaatacctg
      661 ataattatga agctcagggc gtgggtatta atgagttggt caaagctgct gatgagcttg
      721 gaatagaaat acaccgtact gaacgagatg atacagcgat tgcaaaccag ttttttggtg
      781 cagcagaaaa agttgtagga ttaactgaac gtggtgttgc aatattcgca ccacaacttg
      841 acaaacttct gcagaagtat cagaaagttg ggagtaaaat aggaagaacc gctgaaaatg
      901 taggtaataa tctgggaaaa gccggaacag ttctctcagc actacagaat tttacgggga
      961 ttgctttatc aggcatggct cttgatgaat tgctgagaaa acaacgggaa ggagaggata
     1021 taagtcagaa tgatattgcc aaaagtagta ttgaacttat taatcagctt gtagatacag
     1081 tatcaagtat aaacagtacc gttgattcat tttctgagca gcttaaccag cttggctcat
     1141 ttttatccag taaacctcgc ttaagttctg ttggtgggaa attacaaaat ttaccagacc
     1201 tgggctccct gggggatggg ctggatgttg tctccggaat tctttctgct gtatcagcaa
     1261 gctttattct gggaaacagt gacgcacata caggaacaaa agctgcagcg ggtatcgaac
     1321 tgacaactca ggttcttgga aatgttggta aagctgtttc gcaatatatt ctggctcaga
     1381 gaatggcaca ggggttatcg acaacagctg caagtgcggg tctgatcaca tcggctgtta
     1441 tgctggctat cagtcctctt tctttcctgg ctgctgcaga taaatttgag cgagctaagc
     1501 agcttgaatc atattctgaa cgatttaaaa aattgaatta tgaaggggat gctttactcg
     1561 cagcctttca taaagaaacc ggagctatag atgcagccct gacaacaata aatactgtcc
     1621 tgagttctgt atctgcggga gttagtgcag cctccagtgc atccctcata ggggccccga
     1681 taagcatgct ggtgagtgca ttaaccggta cgatatctgg cattctggaa gcatcaaaac
     1741 aggctatgtt tgagcacgtt gcagagaaat tcgctgctcg gatcaatgaa tgggaaaagg
     1801 agcatggcaa aaattatttt gagaatggct atgacgcaag acatgctgcg tttttagaag
     1861 actctctgtc tttgcttgct gatttttctc gtcagcatgc agtagaaaga gcagtcgcaa
     1921 taacccagca acattgggat gagaagatcg gtgaacttgc aggcataacc cgtaatgctg
     1981 atcgcagtca gagtggtaag gcatatatta attatctgga aaatggaggg cttttagagg
     2041 ctcaaccgaa agagtttaca caacaagtgt ttgatcctca aaaagggacc atagaccttt
     2101 caacaggtaa tgtatcaagt gttttgacat ttataacacc aacatttacc ccaggagaag
     2161 aagttagaga aagaaaacag agtggtaaat atgaatatat gacatctctt attgtaaatg
     2221 gtaaggatac atggtctgta aaaggcataa aaaatcataa aggtgtatat gattattcaa
     2281 aattgattca gtttgttgaa aagaataaca aacactatca ggcgagaatg atttctgaac
     2341 tcggagataa agacgatgtg gtttattctg gagcaggctc atcagaagta tttgctggtg
     2401 aaggtcatga taccgtatct tataataaga cggatgttgg taaactaaca attgatgcaa
     2461 caggagcatc aaaacctggt gagtatatag tttcaaaaaa tatgtatggt gacgtgaagg
     2521 tattgcagga agtcgttaag gaacaggagg tgtcagtagg gaagcgaaca gagaaaatac
     2581 aatatcgtga ttttgaattc agaaccggtg gaattcctta tgatgtaata gataatcttc
     2641 attctgttga agagctcatt ggcggaaaac atgatgatga attcaaaggc ggtaagttta
     2701 atgatatatt ccatggcgca gatgggaacg attatatcga aggtaattat ggtaatgatc
     2761 gactatacgg cgatgatggg gatgattata tatccggagg acagggagac gaccagttat
     2821 ttggtggtag tggaaacgat aaattgagtg gaggggatgg taataattat ctgacaggag
     2881 gaagcggtaa tgatgagctt caggcacacg gagcttataa tattctgtca ggtggtactg
     2941 gtgatgataa actttatggt ggtggtggta ttgatcttct ggatggaggg gaaggtaatg
     3001 actatctgaa tggtggtttt ggtaatgata tttatgttta taggcaaaac tatggtcatc
     3061 atacaattgc agatgaagga ggtaaaggag atcgtttgca cttatctgat attagctttg
     3121 atgatatcgc atttaagaga gttggaaatg atcttatcat gaataaagcc attaatggtg
     3181 tactttcatt taatgagtca aatgatgtca atgggataac atttaaaaac tggtttgcga
     3241 aagatgcctc aggagcagat aatcatcttg ttgaggttat aacagataaa gatggtcgag
     3301 aga
//
LOCUS       DAZKHF020000347         3294 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2201S31241
            SAMN43267800-rid25106413.denovo.349, whole genome shotgun sequence.
ACCESSION   DAZKHF020000347 DAZKHF020000000
VERSION     DAZKHF020000347.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267800
            Sequence Read Archive: SRR30307263
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3294)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3294)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 3294)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350581.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350581.2
            Genome Coverage       :: 151x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:21:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,652
            CDSs (total)                      :: 5,557
            Genes (coding)                    :: 5,320
            CDSs (with protein)               :: 5,320
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 237
            CDSs (without protein)            :: 237
            Pseudo Genes (ambiguous residues) :: 0 of 237
            Pseudo Genes (frameshifted)       :: 102 of 237
            Pseudo Genes (incomplete)         :: 130 of 237
            Pseudo Genes (internal stop)      :: 52 of 237
            Pseudo Genes (multiple problems)  :: 41 of 237
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3294
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267800-rid25106413.denovo.349"
                     /serotype="O 26:H11"
                     /isolate="2201S31241"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-01"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            226..1158
                     /gene="rfaD"
                     /locus_tag="ACHOSC_RS22810"
                     /old_locus_tag="pgaptmp_004562"
     CDS             226..1158
                     /gene="rfaD"
                     /locus_tag="ACHOSC_RS22810"
                     /old_locus_tag="pgaptmp_004562"
                     /EC_number="5.1.3.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020233050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008712 - ADP-glyceromanno-heptose
                     6-epimerase activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-glyceromanno-heptose 6-epimerase"
                     /protein_id="HGU4550354.1"
                     /translation="MIIVTGGAGFIGSNIVKALNDKGITDILVVDNLKDGTKFVNLVD
                     LNIADYMDKEDFLIQIMAGEEFGDVEAIFHEGACSSTTEWDGKYMMDNNYQYSKELLH
                     YCLEREIPFLYASSAATYGGRTSDFIESREYEKPLNVYGYSKFLFDEYVRQILPEANS
                     QIVGFRYFNVYGPREGHKGSMASVAFHLNTQLNNGESPKLFEGSENFKRDFVYVGDVA
                     DVNLWFLENGVSGIFNLGTGRAESFQAVADATLAYHKKGQIEYIPFPDKLKGRYQAFT
                     QADLTNLRAAGYDKPFKTVAEGVTEYMAWLNRDA"
     gene            1168..2214
                     /gene="rfaF"
                     /locus_tag="ACHOSC_RS22815"
                     /old_locus_tag="pgaptmp_004563"
     CDS             1168..2214
                     /gene="rfaF"
                     /locus_tag="ACHOSC_RS22815"
                     /old_locus_tag="pgaptmp_004563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-heptose--LPS heptosyltransferase RfaF"
                     /protein_id="HGU4550355.1"
                     /translation="MKILVIGPSWVGDMMMSQSLYRTLQARYPQAIIDVMAPAWCRPL
                     LSRMPEVNEAIPMPLGHGALEIGERRKLGHSLREKRYDRAYVLPNSFKSALVPFFAGI
                     PHRTGWRGEMRYGLLNDVRVLDKEAWPLMVERYIALAYDKGIMRTAQDLPQPLLWPQL
                     QVSEGEKSYTCNQFSLSSERPMIGFCPGAEFGPAKRWPHYHYAELAKQLIDEGYQVVL
                     FGSAKDHEAGNEILAALNTEQQAWCRNLAGETQLDQAVILIAACKAIVTNDSGLMHVA
                     AALNRPLVALYGPSSPDFTPPLSHKARVIRLITGYHKVRKGDAAEGYHQSLIDITPQR
                     VLEELNALLLQEEA"
     gene            2218..3210
                     /gene="rfaC"
                     /locus_tag="ACHOSC_RS22820"
                     /old_locus_tag="pgaptmp_004564"
     CDS             2218..3210
                     /gene="rfaC"
                     /locus_tag="ACHOSC_RS22820"
                     /old_locus_tag="pgaptmp_004564"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008920 - lipopolysaccharide
                     heptosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009244 - lipopolysaccharide core region
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide heptosyltransferase RfaC"
                     /protein_id="HGU4550356.1"
                     /translation="MRVLIVKTSSMGDVLHTLPALTDAQQAIPGIKFDWVVEEGFAQI
                     PSWHAAVERVIPVAIRRWRKAWFSAPIKAERKAFREALQAENYDAVIDAQGLVKSAAL
                     VTRLAHGVKHGLDWQTAREPLASLFYNCKHHIAKQQHAVERTRELFAKSLGYSKPQTQ
                     GDYAIAQHFLTNLPTDAGEYAVFLHATTRDDKHWPEEHWRELIGLLADSGIRIKLPWG
                     APHEEERAKRLAEGFAYVEVLPKMSLEGVARVLAGAKFVVSVDTGLSHLTAALDRPNI
                     TVYGPTDPGLIGGYGKNQVECRSTSMSLADLPAQTVFQNLNLEIITNKLTSEIR"
     gene            3207..>3294
                     /locus_tag="ACHOSC_RS22825"
                     /old_locus_tag="pgaptmp_004565"
     CDS             3207..>3294
                     /locus_tag="ACHOSC_RS22825"
                     /old_locus_tag="pgaptmp_004565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4550357.1"
                     /translation="MTSTLFFSLEKKNWIAYWNRALVFLFITT"
BASE COUNT          836 a          806 c          863 g          789 t
ORIGIN      
        1 aatgcttccg ttatttatca tacatcggaa tattgatact aaagcactat tcacatgcaa
       61 aaccaacatc cgccatgaag gactagctaa aacccaaact agtttgttgc aattagcatc
      121 cttgcacctc tatgtaaagg gctgaaggga ttcggatgtg atggtatgat tacagacatt
      181 cgtgtctgag attgtctctg actccataat tcgaaggtta cagttatgat catcgttacc
      241 ggcggcgcgg gctttatcgg cagcaacatc gttaaagccc tgaatgataa aggcatcacc
      301 gatattctgg tggtggacaa cctgaaagac ggcaccaagt ttgtgaacct ggtggatctg
      361 aatatcgcag actatatgga taaggaagac ttcctgatcc agattatggc tggcgaagag
      421 ttcggcgatg tcgaagcgat tttccacgaa ggcgcgtgct cttccaccac cgagtgggac
      481 ggcaagtata tgatggataa caactatcaa tactccaagg agctgctgca ctactgcctg
      541 gagcgtgaaa tcccgttcct gtacgcttct tccgcagcca cctacggcgg acgcacctcc
      601 gactttattg aatcccgcga gtacgaaaaa ccgttgaacg tctacggtta ctcaaaattc
      661 ctgtttgatg aatatgttcg ccaaatcctg ccggaagcga actcgcagat tgttggcttc
      721 cgctatttca acgtttatgg accgcgtgaa ggccataaag gcagcatggc gagcgtcgct
      781 ttccatctca acacccagct taataacggt gaatcaccga agctgttcga aggtagcgag
      841 aactttaaac gcgacttcgt ctatgttggc gatgtggcag atgtaaacct gtggttcctg
      901 gaaaatggcg tttccggcat cttcaacctc ggcaccggtc gtgcggaatc cttccaggct
      961 gtagctgatg ctacgctggc ttatcacaag aaaggccaga tcgaatacat tccgttcccg
     1021 gataagctga aaggccgcta ccaggcgttc actcaggcag atctgacaaa tctgcgcgcg
     1081 gcgggttacg acaaaccgtt caaaaccgtt gctgaaggtg taacggaata catggcctgg
     1141 ctgaatcgcg acgcataaga gctctgcatg aaaatactgg tgatcggccc gtcttgggtt
     1201 ggcgacatga tgatgtcgca aagtctctat cgcacgctcc aggcgcgcta tccccaggcg
     1261 ataatcgatg tgatggcacc ggcatggtgc cgtccattat tatcgcggat gccggaagtt
     1321 aacgaagcta tccctatgcc tctcggtcac ggagcgctgg aaatcggcga acgccgcaaa
     1381 ctgggtcata gcctgcgtga aaagcgctac gaccgcgcct acgtcttacc taactccttc
     1441 aaatccgcat tagtgccttt cttcgcgggt attcctcatc gcaccggctg gcgcggcgag
     1501 atgcgctacg gtttactcaa cgatgtacgc gtgctcgata aagaagcctg gccgctaatg
     1561 gtggaacgct atatagcgct ggcctatgac aaaggcatta tgcgcacagc acaagatctg
     1621 ccgcagccat tgttatggcc gcagttgcag gtgagcgaag gtgaaaaatc atatacctgt
     1681 aatcaatttt cgctttcatc agaacgtccg atgattggtt tttgcccggg tgcggagttt
     1741 ggtccggcaa aacgctggcc acactaccac tatgcggagc tggcaaagca gctgattgat
     1801 gaaggttatc aggtggttct gtttggctcg gcgaaagatc atgaagcagg caatgagatt
     1861 cttgccgctt taaataccga gcagcaggca tggtgtcgga acctggcggg ggaaacacag
     1921 cttgatcaag cggttatcct gattgcagcc tgtaaagcca ttgtcactaa cgattctggc
     1981 ctgatgcacg ttgcggcggc gctcaatcgt ccgctggttg ccctgtatgg tccaagtagc
     2041 ccggacttca caccgccgct atcccataaa gcgcgcgtga tccgtctgat taccggctat
     2101 cacaaagtgc gtaaaggtga cgctgcggag ggttatcacc agagcttgat cgacattact
     2161 ccccagcgcg tactggaaga actcaacgcg ctattgttac aagaggaagc ctgacggatg
     2221 cgggttttga tcgttaaaac atcgtcgatg ggcgatgttc tccatacgtt gcccgcactc
     2281 actgatgccc agcaggcaat cccagggatt aagtttgact gggtggtgga agaagggttc
     2341 gcacagattc cttcctggca cgctgccgtt gagcgagtta ttcctgtggc aatacgtcgc
     2401 tggcgtaaag cctggttctc ggcccccata aaagctgaac gcaaagcgtt tcgtgaagcg
     2461 ctacaagcag agaactatga cgcagttatc gacgctcagg ggctggtaaa aagcgcggca
     2521 ctggtgacac gtctggcgca tggcgtaaag catggattgg actggcaaac cgctcgcgaa
     2581 cctttagcca gcctgtttta caattgtaag catcatattg caaaacagca gcacgccgta
     2641 gaacgcaccc gcgaactgtt tgccaaaagt ttgggctata gcaaaccgca aacccagggc
     2701 gattatgcta tcgcacagca ttttctgacg aacctgccta cagatgctgg cgaatatgcc
     2761 gtatttcttc atgcgacgac ccgtgatgat aaacactggc cggaagaaca ctggcgagaa
     2821 ttgattggtt tactggctga ttcaggaata cggattaaac ttccgtgggg cgcgccgcat
     2881 gaggaagaac gggcgaaacg actggcggaa ggatttgctt atgttgaagt attgccgaag
     2941 atgagtctgg aaggcgttgc ccgcgtgctg gccggggcta aatttgtagt gtcggtggat
     3001 acggggttaa gccatttaac ggcggcactg gatagaccca atatcacggt ttatggacca
     3061 accgatccgg gattaattgg tgggtatggg aagaatcaag tagagtgtcg ttctacaagt
     3121 atgtctcttg cagatttgcc agctcaaacc gtttttcaaa atttaaatct tgaaataata
     3181 accaataagt tgacatcgga gataagatga cctcaacatt atttttctct ctcgagaaaa
     3241 aaaactggat agcgtactgg aacagagctc tcgtattctt attcattacc acct
//
LOCUS       DAZKHF020000348         3285 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2201S31241
            SAMN43267800-rid25106413.denovo.350, whole genome shotgun sequence.
ACCESSION   DAZKHF020000348 DAZKHF020000000
VERSION     DAZKHF020000348.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267800
            Sequence Read Archive: SRR30307263
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3285)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3285)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 3285)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350581.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350581.2
            Genome Coverage       :: 151x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:21:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,652
            CDSs (total)                      :: 5,557
            Genes (coding)                    :: 5,320
            CDSs (with protein)               :: 5,320
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 237
            CDSs (without protein)            :: 237
            Pseudo Genes (ambiguous residues) :: 0 of 237
            Pseudo Genes (frameshifted)       :: 102 of 237
            Pseudo Genes (incomplete)         :: 130 of 237
            Pseudo Genes (internal stop)      :: 52 of 237
            Pseudo Genes (multiple problems)  :: 41 of 237
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3285
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267800-rid25106413.denovo.350"
                     /serotype="O 26:H11"
                     /isolate="2201S31241"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-01"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            174..2312
                     /gene="nrdD"
                     /locus_tag="ACHOSC_RS22830"
                     /old_locus_tag="pgaptmp_004566"
     CDS             174..2312
                     /gene="nrdD"
                     /locus_tag="ACHOSC_RS22830"
                     /old_locus_tag="pgaptmp_004566"
                     /EC_number="1.17.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008998 - ribonucleoside-triphosphate
                     reductase (thioredoxin) activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic ribonucleoside-triphosphate reductase"
                     /protein_id="HGU4550358.1"
                     /translation="MTPHVMKRDGCKVPFKSERIKEAILRAAKAAEVDDADYCATVAA
                     VVSEQMQGRNQVDINEIQTAVENQLMSGPYKQLARAYIEYRHDRDIEREKRGRLNQEI
                     RGLVEQTNASLLNENANKDSKVIPTQRDLLAGIVAKHYARQHLLPRDVVQAHERGDIH
                     YHDLDYSPFFPMFNCMLIDLKGMLTQGFKMGNAEIEPPKSISTATAVTAQIIAQVASH
                     IYGGTTINRIDEVLAPFVTASYNKHRKTAEEWNIPDAEGYANSRTIKECYDAFQSLEY
                     EVNTLHTANGQTPFVTFGFGLGTSWESRLIQESILRNRIAGLGKNRKTAVFPKLVFAI
                     RDGLNHKKGDPNYDIKQLALECASKRMYPDILNYDQVVKVTGSFKTPMGCRSFLGVWE
                     NENGEQIHDGRNNLGVISLNLPRIALEAKGDEATFWKLLDERLVLARKALMTRIARLE
                     GVKARVAPILYMEGACGVRLNADDDVSEIFKNGRASISLGYIGIHETINALFGGEHVY
                     DNEQLRAKGIAIVERLRQAVDQWKEETGYGFSLYSTPSENLCDRFCRLDTAEFGVVPG
                     VTDKGYYTNSFHLDVEKKVNPYDKIDFEAPYPPLANGGFICYGEYPNIQHNLKALEDV
                     WDYSYQHVPYYGTNTPIDECYECGFTGEFECTSKGFTCPKCGNHDASRVSVTRRVCGY
                     LGSPDARPFNAGKQEEVKRRVKHLGNGQIG"
     gene            2471..2935
                     /gene="nrdG"
                     /locus_tag="ACHOSC_RS22835"
                     /old_locus_tag="pgaptmp_004567"
     CDS             2471..2935
                     /gene="nrdG"
                     /locus_tag="ACHOSC_RS22835"
                     /old_locus_tag="pgaptmp_004567"
                     /EC_number="1.97.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043365 -
                     [formate-C-acetyltransferase]-activating enzyme activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic ribonucleoside-triphosphate
                     reductase-activating protein"
                     /protein_id="HGU4550359.1"
                     /translation="MNYHQYYPVDIVNGPGTRCTLFVSGCVHECPGCYNKSTWRVNSG
                     QTFTKAMEDQIINDLNDTRIKRQGISLSGGDPLHPQNVPDILKLVKRIRAECPGKDIW
                     VWTGYKLDELNAAQMQVVDLINVLVDGKFVQDLKDPSLIWRGSSNQVVHHLR"
     gene            complement(2980..>3285)
                     /gene="cybC"
                     /locus_tag="ACHOSC_RS22840"
                     /old_locus_tag="pgaptmp_004568"
     CDS             complement(2980..>3285)
                     /gene="cybC"
                     /locus_tag="ACHOSC_RS22840"
                     /old_locus_tag="pgaptmp_004568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313240.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA];
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome b562"
                     /protein_id="HGU4550360.1"
                     /translation="NMETLNDNLKVIEKADNAAQVKDALTKMRAAALDAQKATPPKLE
                     DKSPDSPEMKDFRHGFDILVGQIDDALKLANEGKVKEAQAAAEQLKTTRNAYHQKYR"
BASE COUNT          816 a          841 c          879 g          749 t
ORIGIN      
        1 aatatgttgt attaatcgac tataattgct actacagctc cccacgaaaa aggtgcggcg
       61 ttgtggataa gcggatggcg attgcggaaa gcaccggaaa acgaaacgaa aaaaccggaa
      121 aacgcctttc ccaatttctg tggataacct gttcttaaaa atatggagcg atcatgacac
      181 cgcatgtgat gaaacgagac ggctgcaaag tgccgtttaa atcagagcgc atcaaagaag
      241 cgattctgcg tgcagctaaa gcagcggaag tcgatgatgc cgattattgc gccactgttg
      301 ccgcggttgt cagcgagcag atgcagggcc gcaaccaggt ggatatcaac gagatccaga
      361 ccgcagttga aaaccagctg atgtcgggtc catacaaaca actggctcgt gcttacatcg
      421 agtaccgtca cgatcgcgac attgaacgtg aaaaacgcgg tcgcctgaac caggagatcc
      481 gtggtctggt cgagcagacc aacgcctcgt tactcaacga aaacgccaac aaagacagca
      541 aggtgattcc aacccagcgc gacctgctgg ccgggatcgt ggctaaacac tatgcacgtc
      601 agcacctgct gccgcgtgac gtggtgcagg cacatgagcg tggcgatatt cactatcacg
      661 atctcgatta ctcgccgttc ttcccgatgt tcaactgcat gttgatcgac ctgaaaggca
      721 tgctgaccca gggctttaag atggggaacg ccgagattga accgccgaag tcgatctcta
      781 cggcaaccgc ggtaactgcg cagattattg ctcaggtcgc cagccatatt tatggcggca
      841 ccaccattaa ccgtatcgat gaagtgctgg cgccgtttgt taccgccagc tacaacaaac
      901 atcgcaagac cgcagaagag tggaacatcc cggacgccga aggctacgca aactctcgta
      961 ccatcaaaga gtgctacgat gccttccagt cgctggagta cgaagtaaac accctgcaca
     1021 ccgccaacgg tcagacgccg tttgtaacct ttggttttgg tctgggcacc agctgggaat
     1081 cgcgcctgat tcaggaatcg atcctgcgta accgtatcgc aggcctcggt aaaaaccgta
     1141 aaactgcggt gttcccgaaa ctggtgtttg cgattcgcga tggtctgaac cataaaaaag
     1201 gcgatccgaa ctacgacatc aaacagctgg cgctggagtg cgcaagcaag cgcatgtatc
     1261 cggatatcct gaactacgat caggtagtga aagtcaccgg ttcgtttaaa accccgatgg
     1321 gctgccgcag cttcctcggc gtgtgggaaa atgaaaacgg cgagcagatc cacgatggtc
     1381 gtaacaacct cggcgtgatc agcctgaacc tgccgcgtat tgctctggaa gcaaaaggcg
     1441 atgaagccac cttctggaag ctgctggatg aacgtctggt gctggcacgt aaggcgctga
     1501 tgacccgtat cgctcgtctc gaaggcgtga aagcgcgcgt ggccccgatc ctctatatgg
     1561 aaggtgcttg tggcgtacgt ctgaatgctg acgatgatgt ttctgaaatc ttcaaaaacg
     1621 gtcgtgcgtc tatttcgctg ggttacatcg gcatccacga aaccattaac gcgctgttcg
     1681 gcggcgagca tgtttacgac aacgagcagc ttcgcgcgaa aggtatcgcc attgttgaac
     1741 gtctgcgcca ggcagtggat cagtggaaag aagaaacggg ttatggtttc agtctctaca
     1801 gcacgccgag tgaaaacctg tgcgaccgct tctgccgtct cgatactgct gagtttggcg
     1861 tggtgccggg cgtgaccgat aaaggttact acaccaacag tttccacctc gatgtggaga
     1921 agaaggtgaa cccgtacgac aagatcgact ttgaagcgcc ttacccgccg ctggcgaacg
     1981 gtggtttcat ttgctacggc gagtatccaa acattcagca caacctgaag gcgctggaag
     2041 atgtctggga ttacagctat cagcatgtac cgtattacgg caccaataca ccgattgatg
     2101 agtgctacga gtgtggcttt accggtgagt tcgagtgcac cagcaaaggc ttcacttgcc
     2161 cgaaatgtgg taaccatgac gcctcccgtg tgtcggtgac tcgccgcgtg tgcggatatt
     2221 taggtagccc ggatgcacgt ccatttaacg ccggtaagca ggaagaagtt aagcgccgcg
     2281 ttaaacattt ggggaatggg cagataggtt aatcttcact ctacgttaag caaatgccgg
     2341 atgcggcgta aacgccttat ccggcctaca tggcaacaac gattgtaggt ctgataagac
     2401 gcgacagcgt cgcatcaggc gttgattacc ggatgcatcc tatacgcgga ctgataagcc
     2461 aggcgaaaca atgaattatc atcagtacta tcctgtcgac atcgtcaacg gccccggcac
     2521 tcgctgcacc ctgtttgtct ccgggtgtgt tcatgaatgc cccggttgct ataacaaaag
     2581 cacatggcgg gtaaattccg gtcagacatt tactaaagca atggaagacc agattattaa
     2641 cgatctgaat gacactcgta tcaaacgcca gggtatttcc ctctccggcg gcgatccgct
     2701 gcatccgcaa aacgtgccgg atattctgaa actggtaaaa cgcatccgcg ccgagtgtcc
     2761 gggtaaagac atctgggtgt ggacaggcta taaactcgac gaactcaacg ctgcgcaaat
     2821 gcaggttgtt gatttgatta acgtactggt cgacggcaaa tttgtgcagg atttaaaaga
     2881 cccttccctg atctggcgcg gcagcagcaa tcaggtggtg catcatttgc gatgataagt
     2941 tccctgttac ctgagtgcag gcaacaggga aatgaggaat taacgatact tctggtgata
     3001 ggcgttgcgg gtcgttttca gttgctctgc agcagcctgc gcttctttta ctttaccttc
     3061 atttgccagc ttcagcgcgt cgtcaatctg accgaccaga atgtcgaaac cgtggcggaa
     3121 atctttcatt tccgggctgt ccggtgattt atcttcgagc ttcggcggcg ttgctttttg
     3181 cgcatccagc gctgcggcgc gcatcttcgt taacgcgtct ttgacttgcg ccgcgttatc
     3241 cgctttttcg atcactttta aattgtcgtt gagggtttcc atatt
//
LOCUS       DAZKHF020000349         3282 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2201S31241
            SAMN43267800-rid25106413.denovo.351, whole genome shotgun sequence.
ACCESSION   DAZKHF020000349 DAZKHF020000000
VERSION     DAZKHF020000349.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267800
            Sequence Read Archive: SRR30307263
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3282)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3282)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 3282)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350581.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350581.2
            Genome Coverage       :: 151x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:21:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,652
            CDSs (total)                      :: 5,557
            Genes (coding)                    :: 5,320
            CDSs (with protein)               :: 5,320
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 237
            CDSs (without protein)            :: 237
            Pseudo Genes (ambiguous residues) :: 0 of 237
            Pseudo Genes (frameshifted)       :: 102 of 237
            Pseudo Genes (incomplete)         :: 130 of 237
            Pseudo Genes (internal stop)      :: 52 of 237
            Pseudo Genes (multiple problems)  :: 41 of 237
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3282
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267800-rid25106413.denovo.351"
                     /serotype="O 26:H11"
                     /isolate="2201S31241"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-01"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            complement(91..618)
                     /gene="mobB"
                     /locus_tag="ACHOSC_RS22845"
                     /old_locus_tag="pgaptmp_004569"
     CDS             complement(91..618)
                     /gene="mobB"
                     /locus_tag="ACHOSC_RS22845"
                     /old_locus_tag="pgaptmp_004569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418293.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-guanine dinucleotide biosynthesis
                     protein MobB"
                     /protein_id="HGU4550361.1"
                     /translation="MAGKTMIPLLAFAAWSGTGKTTLLKQLIPALCARGIRPGLIKHT
                     HHDMDVDKPGKDSYELRKAGAAQTIVASQQRWALMTETPDEEELDLQFLASRMDTSKL
                     DLILVEGFKHEEIAKIVLFRDGAGHRPEELVIDRHVIAVASDVPLNLDVALLDINDVE
                     GLADFVVEWMQKQNG"
     gene            complement(600..1184)
                     /gene="mobA"
                     /locus_tag="ACHOSC_RS22850"
                     /old_locus_tag="pgaptmp_004570"
     CDS             complement(600..1184)
                     /gene="mobA"
                     /locus_tag="ACHOSC_RS22850"
                     /old_locus_tag="pgaptmp_004570"
                     /EC_number="2.7.7.77"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum cofactor guanylyltransferase MobA"
                     /protein_id="HGU4550362.1"
                     /translation="MNLMTTITGVVLAGGKARRMGGVDKGLLELNGKPLWQHVADALM
                     TQLSHVVVNANRHQEIYQASGLKVIEDSLADYPGPLAGMLSVMQQEAGEWFLFCPCDT
                     PYIPEDLAARLNHQRKDAPVVWVHDGERDHPTIALVNRAIEPLLLEYLQAGERRVMAF
                     MRLAGGHAVDFSDNKDAFINVNTPEELARWQEKR"
     gene            1254..1523
                     /locus_tag="ACHOSC_RS22855"
                     /old_locus_tag="pgaptmp_004571"
     CDS             1254..1523
                     /locus_tag="ACHOSC_RS22855"
                     /old_locus_tag="pgaptmp_004571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015966571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YihD family protein"
                     /protein_id="HGU4550363.1"
                     /translation="MKCKRLNEVIELLQPAWQKEPDLNLLQFLQKLAKESGFDGELAD
                     LTDDILIYHLKMRDSAKDAVIPGLQKDYEEDFKTALLRARGVIKE"
     gene            1600..2586
                     /gene="srkA"
                     /locus_tag="ACHOSC_RS22860"
                     /old_locus_tag="pgaptmp_004572"
     CDS             1600..2586
                     /gene="srkA"
                     /locus_tag="ACHOSC_RS22860"
                     /old_locus_tag="pgaptmp_004572"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stress response kinase SrkA"
                     /protein_id="HGU4550364.1"
                     /translation="MNNSAFTFQTLHPDTIMDALFEQGIRVDSGLTPLNSYENRVYQF
                     QDEDRRRFVVKFYRPERWTADQILEEHQFALQLVNDEVPVAAPVAFNGQTLLNHQGFY
                     FAVFPSVGGRQFEADNIDQMEAVGRYLGRMHQTGRKQLFIHRPTIGLNEYLIEPRKLF
                     EDATLIPSGLKAAFLKATDELIAAVTAHWREDFTVLRLHGDCHAGNILWRDGPMFVDL
                     DDARNGPAIQDLWMLLNGDKAEQRMQLETIIEAYEEFSEFDTAEIGLIEPLRAMRLVY
                     YLAWLMRRWADPAFPKNFPWLTGEDYWLRQTATFIEQAKVLQEPPLQLTPMY"
     gene            2603..3229
                     /gene="dsbA"
                     /locus_tag="ACHOSC_RS22865"
                     /old_locus_tag="pgaptmp_004573"
     CDS             2603..3229
                     /gene="dsbA"
                     /locus_tag="ACHOSC_RS22865"
                     /old_locus_tag="pgaptmp_004573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiol:disulfide interchange protein DsbA"
                     /protein_id="HGU4550365.1"
                     /translation="MKKIWLALAGLVLAFSASAAQYEDGKQYTTLEKPVAGAPQVLEF
                     FSFFCPHCYQFEEVLHISDNVKKKLPEGVKMTKYHVNFMGGDLGKDLTQAWAVAMALG
                     VEDKVTVPLFEGVQKTQTIRSASDIRDVFINAGIKGEEYDAAWNSFVVKSLVAQQEKA
                     AADVQLRGVPAMFVNGKYQLNPQGMDTSNMDVFVQQYADTVKYLSEKK"
BASE COUNT          830 a          785 c          797 g          870 t
ORIGIN      
        1 gaaggccatc cgtcaggatg gcctttttgc attggcgcag aaaaaaatgc cggatgcgac
       61 gctggcgcgt cttatccagc ctactcttgc ttatccgttc tgcttttgca tccactccac
      121 cacaaaatca gccagcccct caacatcatt gatatccagt aatgcgacat caagattaag
      181 cggcacatca ctggctacag caataacatg cctgtctatc actaattctt caggtcgatg
      241 tccggctcca tcgcgaaaca gcacaatctt tgcgatctct tcatgcttaa acccttcgac
      301 cagaatcaaa tccagctttg aggtatccat tcgacttgcg agaaactgta gatccagctc
      361 ttcttcgtct ggtgtttccg tcatcaaggc ccatcgttgc tggctggcaa cgatggtttg
      421 tgccgcgcca gccttgcgca gctcatagct atctttgcct ggcttatcaa catccatatc
      481 atggtgcgta tgcttaatca gccctggacg gatccctctg gcgcataatg ccgggatcag
      541 ttgtttcaaa agcgtagttt ttccggtacc actccacgcg gcaaaggcga gtaacggtat
      601 catcgttttt cctgccatcg ggcaagctcc tctggcgtat tcacgttaat aaatgcatct
      661 ttattatcgc tgaaatcaac cgcatgaccg ccagccagac gcataaatgc cattacccgg
      721 cgttctcctg cttgcagata ttccagtaat aaaggctcaa ttgcgcggtt taccagagca
      781 atagtcgggt gatcgcgttc accgtcgtgg acccacacga caggcgcatc tttgcgctga
      841 tgattaagcc gggctgctaa atcctcggga atataaggag tatcgcacgg acaaaacaaa
      901 aaccactcgc ctgcttcctg ctgcattact gaaagcattc ctgccagagg gcctgggtaa
      961 tccgccagtg aatcttcaat cactttcaga ccgcttgcct gatagatttc ctgatggcga
     1021 ttggcattaa ccacgacgtg agagagctgc gtcataagcg cgtcagcgac atgttgccat
     1081 aatggtttgc cgtttaattc aagcaatcct ttatctacgc cgcccattcg tctggcttta
     1141 ccgcctgcca gcacaacgcc tgttatcgtc gtcatcagat tcaccgatat cgcctctttt
     1201 attgtgggat tgaccctgct aacgtgtctg tctcaagagt aaggagcatt actatgaaat
     1261 gtaaacgtct gaatgaagtt attgaactcc tccagccagc ctggcaaaaa gagccagacc
     1321 ttaacctgtt gcaatttttg cagaaactgg cgaaagagtc aggttttgac ggcgaactgg
     1381 cggatttgac ggatgacatt ctgatctatc acctgaaaat gcgtgattcc gcgaaagatg
     1441 cggtgatccc gggtttgcag aaagattatg aagaagactt caaaacggcg ctgttacgcg
     1501 ctcgcggcgt aattaaagag taaaagcttg taagcggtgc caccaaaatc atcgtgaaat
     1561 gatatccttc atcattcgta atgttttccg gatgatggga tgaacaacag cgcttttact
     1621 ttccagacac tacacccgga taccatcatg gatgctctat ttgagcaagg gatccgggtg
     1681 gattccggtc ttaccccgct taacagctat gaaaaccgtg tctatcaatt tcaggacgaa
     1741 gatcgtcgac gttttgtcgt caaattttat cgccctgaac gttggacagc cgatcaaatc
     1801 ctcgaagaac atcaatttgc gttgcagttg gtaaatgatg aagttccggt cgcagcacct
     1861 gtggccttta acggtcagac tttattgaat catcagggat tttatttcgc tgtttttcca
     1921 agcgtcggtg gtcgccagtt cgaagctgat aatatcgatc agatggaagc ggttgggcgt
     1981 tatttagggc gtatgcacca gacggggcgc aaacagcttt ttatccatcg cccgaccatc
     2041 ggtctgaatg aatatctcat tgagccacgc aagctgtttg aggacgctac actgatacct
     2101 tccgggttga aagcggcatt cctgaaagcg acagatgagc tgatcgccgc cgttacagcg
     2161 cactggcggg aagatttcac cgttctgcgg ctacatggag actgccacgc cgggaatatt
     2221 ctctggcgcg atggtccaat gtttgttgat ctggatgatg cacgtaatgg tccagctatt
     2281 caggatttgt ggatgttgct caatggcgat aaagccgagc agcggatgca actggaaact
     2341 attattgaag cttatgaaga atttagcgag ttcgacaccg ctgaaatcgg actgattgaa
     2401 cctttacgcg ccatgcgttt ggtttattat cttgcctggc taatgcggcg ttgggctgat
     2461 cccgcgttcc cgaaaaattt cccgtggtta accggggaag attactggct gcgacaaacg
     2521 gcgactttta tagaacaggc aaaagttcta caagaacccc ctttacaatt aacacctatg
     2581 tattaatcgg agagagtaga tcatgaaaaa gatttggctg gcgctggctg gtttagtttt
     2641 agcgtttagc gcatcggcgg cgcagtatga agatggtaaa cagtacacta ccctggaaaa
     2701 accggtagct ggcgcgccgc aagtgctgga gtttttctct ttcttctgtc cgcactgcta
     2761 tcagtttgaa gaagttctgc atatttctga taatgtgaag aaaaaactgc cggaaggcgt
     2821 gaagatgact aaataccacg tcaacttcat gggtggtgac ctgggcaaag atctgactca
     2881 ggcatgggct gtggcgatgg cgctgggcgt ggaagacaaa gtcactgttc cgctgtttga
     2941 aggcgtacag aaaacccaga ccattcgttc agcatctgat atccgcgatg tatttatcaa
     3001 cgcaggtatt aaaggtgaag agtacgacgc ggcgtggaac agcttcgtgg tcaaatctct
     3061 ggtcgctcag caggaaaaag ctgcagctga cgtgcaattg cgtggcgttc cggcgatgtt
     3121 tgttaacggt aaatatcagc tgaatccgca gggtatggat accagcaata tggatgtttt
     3181 tgttcagcag tatgctgata cagtgaaata tctgtccgag aaaaaataat tcaatgtaaa
     3241 ttacataagg cccgtgaata ttcacgggct ttttttatta tt
//
LOCUS       DAZKHF020000350         3252 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2201S31241
            SAMN43267800-rid25106413.denovo.352, whole genome shotgun sequence.
ACCESSION   DAZKHF020000350 DAZKHF020000000
VERSION     DAZKHF020000350.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267800
            Sequence Read Archive: SRR30307263
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3252)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3252)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 3252)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350581.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350581.2
            Genome Coverage       :: 151x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:21:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,652
            CDSs (total)                      :: 5,557
            Genes (coding)                    :: 5,320
            CDSs (with protein)               :: 5,320
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 237
            CDSs (without protein)            :: 237
            Pseudo Genes (ambiguous residues) :: 0 of 237
            Pseudo Genes (frameshifted)       :: 102 of 237
            Pseudo Genes (incomplete)         :: 130 of 237
            Pseudo Genes (internal stop)      :: 52 of 237
            Pseudo Genes (multiple problems)  :: 41 of 237
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3252
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267800-rid25106413.denovo.352"
                     /serotype="O 26:H11"
                     /isolate="2201S31241"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-01"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            complement(609..806)
                     /locus_tag="ACHOSC_RS22870"
                     /old_locus_tag="pgaptmp_004574"
     CDS             complement(609..806)
                     /locus_tag="ACHOSC_RS22870"
                     /old_locus_tag="pgaptmp_004574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HGU4550366.1"
                     /translation="MLTTTSHDSVFLRADNSLIDMNYITSFTGMTDKWFYKLISEGHF
                     PKPIKLGRSSRWYKSEVEQWK"
     gene            complement(872..1142)
                     /locus_tag="ACHOSC_RS22875"
                     /old_locus_tag="pgaptmp_004575"
                     /pseudo
     CDS             complement(872..1142)
                     /locus_tag="ACHOSC_RS22875"
                     /old_locus_tag="pgaptmp_004575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001593693.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            1447..1635
                     /locus_tag="ACHOSC_RS22880"
                     /old_locus_tag="pgaptmp_004576"
     CDS             1447..1635
                     /locus_tag="ACHOSC_RS22880"
                     /old_locus_tag="pgaptmp_004576"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4550367.1"
                     /translation="MTNEELQAIALSMLERQREKVHQQRESLLNPSPFTRPKRLELEA
                     FLDKYPRRLKSGKTRPPG"
     gene            1963..2157
                     /locus_tag="ACHOSC_RS22885"
                     /old_locus_tag="pgaptmp_004577"
     CDS             1963..2157
                     /locus_tag="ACHOSC_RS22885"
                     /old_locus_tag="pgaptmp_004577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4550368.1"
                     /translation="MNENKIKRLEQLLQARQREFATKWGRAALPHERLTYFPLEDLIK
                     NNGKKIRSDKKHFLECNEDK"
     gene            2500..2628
                     /locus_tag="ACHOSC_RS22890"
                     /old_locus_tag="pgaptmp_004578"
     CDS             2500..2628
                     /locus_tag="ACHOSC_RS22890"
                     /old_locus_tag="pgaptmp_004578"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4550369.1"
                     /translation="MGADVTEFSVQGKSGTCRQFSDLFNREIISYSLSERSVMEHR"
     gene            complement(2938..>3252)
                     /locus_tag="ACHOSC_RS22895"
                     /old_locus_tag="pgaptmp_004579"
     CDS             complement(2938..>3252)
                     /locus_tag="ACHOSC_RS22895"
                     /old_locus_tag="pgaptmp_004579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4550370.1"
                     /translation="YEKRTGSASSASIIIQKELQPDNAIIQGKAISSVGRNVEISTGK
                     RGTDINTRRIPASNCLSARNAELKHNHTGARILWSYAFSIVTTNRNKNLAGANIKRCD
                     TV"
BASE COUNT          957 a          746 c          720 g          829 t
ORIGIN      
        1 tcaggataca gtttgtagga gctttctttt ttgaacaatg atttgcagga cagttatgct
       61 aaactagcgg aatcagtatc acaacaataa atgactgatc atacctgaaa tttgaattaa
      121 caggcatcat gaatgtcaat agtctaagcg gtgactactt atatcgcttg cataaatact
      181 aaacactgac tagaaaagta ttggataacc ccgtcactaa aagtaacgct tcggttattt
      241 gctcggaatg tattaacgcc aagattgtta gccgttacgc cttatatcac tggcgctgac
      301 aaatgtttct tttctaattt actgaaccgg cgcagacacg caggaaagca cgccggcacc
      361 ggtgtaatgc cagttctaat caataagaat ggaatgacga atcagtcagg aagggataac
      421 aacccgctgc tgaacggaag gtatcagcag gaagatgaac gggaatatcg ccagcaccaa
      481 gggcagggcc gggttccaca ataccagcgc tggcattttg cacattgcca gcgtaccggc
      541 attacaacac gtttcatgct gctgcccccc aggattcctc gattcgttgc tacatccact
      601 gctctacttc acttccactg ctccacttca cttttgtacc agcggctgct gcgccccagt
      661 ttgatgggtt tagggaaatg gccttcactg atcaacttgt aaaaccattt gtcggtcata
      721 ccggtgaaac tggtgatata gttcatgtcg atcagggaat tatcggcacg cagaaatacg
      781 ctgtcgtggc ttgttgtagt caacatggtc agaatgtcct gtgagattta tgaagattgt
      841 gcgaatgcgg gaaatctact gagctgtgct ttcagaactg gcctgttacg ggataccagg
      901 gattaccggc ggggtaacgg gcttccggat catacacacc acgattatcg cggacaaaat
      961 cactgaacgc ccatatcacc tctttaagta tgtcttcgca gtccggtaaa tgacgatcca
     1021 gcgccacatc ccgggtggta cagctttgat gcgcccggtg acacaaagcc cggattgttc
     1081 cagacatcct gaatcaaacg ccccagatta ggggcgtcga aatatgcctc tctgaccatt
     1141 atattccggc gtataggtag caggtctgaa gtgacaatgc gtcacctgac gttaaaagtc
     1201 acccaagatg accccagtgg gggcaacaag tataaaaccg ttttttgatt aagtggtcgg
     1261 atatgaaaag gggaaggttt atgtgaaaaa tacctgtgtt ttctttgaaa acacacaaca
     1321 gtatcaggtt atttatgttt atacagtgcg ttccaaactg acctgaataa ttacgcaagt
     1381 tgcaacaata aaacattttt gccgtagtgt gaggtactcc ggtaaattgc ttcccaggtg
     1441 cagacaatga ctaacgaaga actacaggca atcgcattat ccatgcttga gcgccagcgc
     1501 gaaaaagtac accagcaacg agagtctttg cttaacccat ctccattcac caggccaaaa
     1561 cgattggagc ttgaagcatt cttggacaaa tacccacggc ggcttaaatc aggcaaaacc
     1621 agaccaccag gctgatagct cacagtttgt tctccgattc aataaaacgg ataagaacca
     1681 tcagcgcggt agcgtaaggc actacagcgc attgctttac gagtaataac aatggattta
     1741 tcaccagaag acgcaaagtt tttacgagaa ctgcaacacc gtatgctcag agaagccatt
     1801 atcgaacagc aaacgggcat gagcgccata gacccattgc gatacaaacg ggcctcatct
     1861 cctttagttg acgacaattc caatgcggat aaaacacgtc caccacactg attacatttt
     1921 tcattagcac agtggtgtga tgcagcatac gaggaaaacc ggatgaatga gaacaaaata
     1981 aaaaggctgg agcaactgtt acaggcacgc caacgggagt ttgccactaa atggggacgt
     2041 gccgcgctgc cacatgaacg cctaacctat tttccacttg aagacctcat taaaaataat
     2101 ggcaaaaaaa tcaggtctga taagaaacac ttcttagaat gcaatgaaga caaataacaa
     2161 caatctgcat attgccacca accacagatg tggcatcgtt atcagcgcac ttgattttct
     2221 ttatatccac tgacgagttt gccggtcatt ctggtatcat gcagatattt acggcgtaca
     2281 agcacactag catgacattg taataaggcc tgagcctaac gctttaatgt gtgtcataca
     2341 ctcagtggcg taatcgttga caatatggtg catgtaatcg taagcgtcca gcgaaatcca
     2401 tctggtcata atctgaagca tccgacaaag tggtgtccac caaataagtg atctgcgccc
     2461 aatatcctga gccggaactt tgacaaaggc caacgaaaaa tgggagcgga tgttacagag
     2521 ttctcggtac aggggaaaag tggtacctgt cgccagttct ccgatctgtt taaccgggaa
     2581 attatctcct acagcttgtc agaaaggtcg gtgatggagc atcgttaata cgatgctata
     2641 cgatgcattc acagtgcccg gccagaggat gccccgtcgc tgcatatgga tcagggttgg
     2701 caatatcgaa ttgcaggcta taggcaaagt taaagcccca tggagtagca caaaatatgc
     2761 cgcacaaagg aaacggtctg aataacgcag tgatgaagaa cttcttcagc acactgctaa
     2821 aacgcatagt gatcgagcgc tgattctggc gaacaactga actaatacat cagaatctgc
     2881 attatgttaa ataaatataa aaagatggtt taaatacccc gttacttttg acttacacta
     2941 tacggtatcg catcgtttaa tattcgcacc ggccagattt ttatttctat tagttgtcac
     3001 aatactgaat gcgtacgacc acagtattct ggctcctgtg tggttatgct ttaattctgc
     3061 gttccgggca gataagcagt tgcttgcagg aatccttctt gtgttaatgt cagttcccct
     3121 tttaccagtg ctgatttcca cattccgtcc aacagagctt atagcctttc cctggattat
     3181 agcattgtcc ggctgaagtt ctttttgaat aataatagaa gcactgctgg cagatccagt
     3241 ccgtttttca ta
//
LOCUS       DAZKHF020000351         3246 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2201S31241
            SAMN43267800-rid25106413.denovo.353, whole genome shotgun sequence.
ACCESSION   DAZKHF020000351 DAZKHF020000000
VERSION     DAZKHF020000351.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267800
            Sequence Read Archive: SRR30307263
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3246)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3246)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 3246)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350581.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350581.2
            Genome Coverage       :: 151x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:21:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,652
            CDSs (total)                      :: 5,557
            Genes (coding)                    :: 5,320
            CDSs (with protein)               :: 5,320
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 237
            CDSs (without protein)            :: 237
            Pseudo Genes (ambiguous residues) :: 0 of 237
            Pseudo Genes (frameshifted)       :: 102 of 237
            Pseudo Genes (incomplete)         :: 130 of 237
            Pseudo Genes (internal stop)      :: 52 of 237
            Pseudo Genes (multiple problems)  :: 41 of 237
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3246
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267800-rid25106413.denovo.353"
                     /serotype="O 26:H11"
                     /isolate="2201S31241"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-01"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            38..1189
                     /gene="yiaY"
                     /locus_tag="ACHOSC_RS22900"
                     /old_locus_tag="pgaptmp_004580"
     CDS             38..1189
                     /gene="yiaY"
                     /locus_tag="ACHOSC_RS22900"
                     /old_locus_tag="pgaptmp_004580"
                     /EC_number="1.1.1.103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonine dehydrogenase"
                     /protein_id="HGU4550371.1"
                     /translation="MASSTFFIPSVNVIGADSLTDAMNMMADYGFTRTLIVTDSMLTK
                     LGMAGDVQKALEERNIFSVIYDGTQPNPTTENVAAGLKLLKENNCDSVISLGGGSPHD
                     CAKGIALVAANGGDIRDYEGVDRSAKPQLPMIAINTTAGTASEMTRFCIITDEARHIK
                     MAIVDKHVTPLLSVNDSSLMIGMPKSLTAATGMDALTHAIEAYVSIAATPITDACALK
                     AVTMIAENLPLAVEDGSNAKAREAMAYAQFLAGMAFNNASLGYVHAMAHQLGGFYNLP
                     HGVCNAVLLPHVQVFNSKVAAARLRDCAAAMGVNVTGKNDAEGAEACINAIRELAKKV
                     DIPAGLRDLNVKEEDFAVLATNALKDACGFTNPIQATHEEIVAIYRAAM"
     gene            1354..2892
                     /gene="aldB"
                     /locus_tag="ACHOSC_RS22905"
                     /old_locus_tag="pgaptmp_004581"
     CDS             1354..2892
                     /gene="aldB"
                     /locus_tag="ACHOSC_RS22905"
                     /old_locus_tag="pgaptmp_004581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001551836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase AldB"
                     /protein_id="HGU4550372.1"
                     /translation="MTNNPPSAQIKPGEYGFPLKLKARYDNFIGGEWVAPADGEYYQN
                     LTPVTGQLLCEVASSGKRDIDLALDAAHKVKDKWAHTSVQDRAAILFKIADRMEQNLE
                     LLATAETWDNGKPIRETSAADVPLAIDHFRYFASCIRAQEGGISEVDSETVAYHFHEP
                     LGVVGQIIPWNFPLLMASWKMAPALAAGNCVVLKPARLTPLSVLLLMEIVGDLLPPGV
                     VNVVNGAGGEIGEYLATSKRIAKVAFTGSTEVGQQIMQYATQNIIPVTLELGGKSPNI
                     FFADVMDEEDAFFDKALEGFALFAFNQGEVCTCPSRALVQESIYERFMERAIRRVESI
                     RSGNPLDSVTQMGAQVSHGQLETILNYIDIGKKEGADVLTGGRRKLLEGELKDGYYLE
                     PTILFGQNNMRVFQEEIFGPVLAVTTFKTMEEALELANDTQYGLGAGVWSRNGNLAYK
                     MGRGIQAGRVWTNCYHAYPAHAAFGGYKQSGIGRETHKMMLEHYQQTKCLLVSYSDKP
                     LGLF"
BASE COUNT          783 a          813 c          886 g          764 t
ORIGIN      
        1 agctgcccac ccttttttac actttcagga gtgtgttatg gcatcttcaa ctttctttat
       61 tccttctgtg aatgtcatcg gcgctgattc attgactgat gcaatgaata tgatggcaga
      121 ttatggattt acccgtacct taattgtcac tgacagtatg ttaacgaaat taggtatggc
      181 gggtgatgtg caaaaagcac tggaagaacg caatattttt agcgttattt atgatggcac
      241 ccaacctaac cccaccacgg aaaacgtcgc cgcaggtttg aaattactta aagaaaataa
      301 ttgcgatagc gtgatctcct taggcggtgg ttctccacac gactgcgcaa aaggtattgc
      361 gctggtggca gccaatggcg gcgatattcg cgattacgaa ggcgttgacc gctctgcaaa
      421 accgcagctg ccgatgatcg ccatcaatac cacggcgggt acggcctctg aaatgacccg
      481 tttctgcatc atcactgacg aagcgcgtca tatcaaaatg gcgattgttg ataaacatgt
      541 cactccgctg ctttctgtca atgactcctc tctgatgatt ggtatgccga agtcactgac
      601 cgccgcaacg ggtatggatg ccttaacgca cgctatcgaa gcgtatgttt ctattgccgc
      661 cacgccgatc actgacgctt gtgcactgaa agccgtgacc atgattgccg aaaacctgcc
      721 gttagccgtt gaagatggca gtaatgcgaa agcgcgtgaa gcaatggctt atgcccagtt
      781 cctcgccggt atggcgttca ataatgcttc tctgggttat gttcatgcga tggcgcacca
      841 gctgggcggt ttctacaacc tgccacacgg tgtatgtaac gccgttttgc tgccgcatgt
      901 tcaggtattc aacagcaaag tcgccgccgc acgtctgcgt gactgtgccg ctgcaatggg
      961 cgtgaacgtg acaggtaaaa acgatgcgga aggtgctgaa gcctgcatta acgccatccg
     1021 tgaactggcg aagaaagtgg atatcccggc aggcctacgc gacctgaacg tgaaagaaga
     1081 agatttcgcg gttctggcga ctaatgccct gaaagatgcc tgtggtttta ctaacccgat
     1141 ccaggcaact cacgaagaaa ttgtggcgat ttatcgcgca gcgatgtaat catcatttcc
     1201 acaacggctg gcaaattgtc agccgctttt tcaactatct ctgtaaccct tgcccgtaaa
     1261 ttcgtgatag ctgtcgtaaa gctgttaccg actggcgaag atttcgccag tcacgtctac
     1321 ccttgttata cctcacaccg caaggagacg atcatgacca ataatccccc ttcagcacag
     1381 attaagcccg gcgagtatgg tttccccctc aagttaaaag cccgctatga caactttatt
     1441 ggcggcgaat gggtagcccc tgccgacggc gagtattacc agaatctgac gccggtgacc
     1501 gggcagctgc tgtgcgaagt ggcgtcttcg ggcaaacgag acatcgatct ggcgctggat
     1561 gctgcgcaca aagtgaaaga taaatgggcg cacacctcgg tgcaggatcg cgcggcgatt
     1621 ctgtttaaga ttgccgatcg aatggaacaa aaccttgagc tgttagcgac ggctgaaacc
     1681 tgggataacg gcaaacccat tcgcgaaacc agtgctgccg acgtgccgct ggcgattgac
     1741 cattttcgct atttcgcctc gtgtattcgg gcgcaggaag gtgggatcag tgaagttgat
     1801 agcgaaaccg tggcctatca tttccatgaa ccgttaggcg tggtggggca gattatcccg
     1861 tggaacttcc cgctgctgat ggcgagctgg aaaatggctc ccgcgctggc ggcgggcaac
     1921 tgtgtggtgc tgaaacccgc acgtcttacc ccgctttctg tactgctgct aatggaaatc
     1981 gtcggtgatt tactgccgcc gggcgtggtg aacgtggtca acggcgcagg tggggaaatt
     2041 ggcgaatatc tggcgacctc gaaacgcatc gccaaagtgg cgtttaccgg ctcaacggaa
     2101 gtgggccaac aaattatgca atacgccacg caaaacatta ttccggtgac gctggagctg
     2161 ggcggcaaat cgccaaatat cttctttgct gatgtgatgg atgaagaaga tgcctttttc
     2221 gataaagcgc tggaaggctt tgcactgttt gcctttaacc agggtgaagt ttgcacctgt
     2281 ccgagtcgtg ccttagtgca ggagtcaatc tacgaacgct ttatggaacg cgccatccgc
     2341 cgtgtcgaaa gcattcgcag cggtaaccca ctcgacagcg tgacgcaaat gggcgcgcag
     2401 gtttctcacg ggcaactgga aaccatcctc aactacattg atatcggtaa aaaagagggc
     2461 gcagacgtgc tcaccggcgg gcggcgcaag ctgctggaag gtgaactgaa agacggctac
     2521 tacctcgaac cgacgattct gtttggtcag aacaatatgc gggtgttcca ggaggagatt
     2581 tttggcccgg tgctggcggt gaccaccttc aaaacgatgg aagaagcgct ggagctggcg
     2641 aacgatacgc aatatggcct gggcgcgggc gtctggagcc gcaacggtaa tctggcctat
     2701 aagatggggc gcggcataca ggctgggcgc gtgtggacca actgctatca cgcttacccg
     2761 gcacatgctg cgtttggtgg ctacaaacaa tcaggtatcg gtcgcgaaac ccacaagatg
     2821 atgctggagc attaccagca aaccaagtgc ctgctggtga gctactcgga taaaccgttg
     2881 gggctgttct gatataagaa gctggtcgca ttgggtattc attgcctgat gcgacgctta
     2941 cgcgtcttat catgcctacg ggaacctgac cgtaggccgg ataaggcgta cacgccgcat
     3001 ccggcataaa caaagcgcac gttgttacaa tttacgtttt aaacctacgt gcgcaataac
     3061 ccagactacg ccaacagaag tgcgtttctg gggcgtgttt tgccaggagt ttactctcgc
     3121 gtttcgatgg tgagcgggca aaccaggccg taaatcacca ttggcgttcc gaggccaatc
     3181 agcaataaac ggtagataat tgaaggttta atacgcttta tctgtactgt ggatgtttta
     3241 ctggag
//
LOCUS       DAZKHF020000352         3237 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2201S31241
            SAMN43267800-rid25106413.denovo.354, whole genome shotgun sequence.
ACCESSION   DAZKHF020000352 DAZKHF020000000
VERSION     DAZKHF020000352.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267800
            Sequence Read Archive: SRR30307263
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3237)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3237)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 3237)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350581.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350581.2
            Genome Coverage       :: 151x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:21:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,652
            CDSs (total)                      :: 5,557
            Genes (coding)                    :: 5,320
            CDSs (with protein)               :: 5,320
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 237
            CDSs (without protein)            :: 237
            Pseudo Genes (ambiguous residues) :: 0 of 237
            Pseudo Genes (frameshifted)       :: 102 of 237
            Pseudo Genes (incomplete)         :: 130 of 237
            Pseudo Genes (internal stop)      :: 52 of 237
            Pseudo Genes (multiple problems)  :: 41 of 237
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3237
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267800-rid25106413.denovo.354"
                     /serotype="O 26:H11"
                     /isolate="2201S31241"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-01"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            complement(<1..112)
                     /locus_tag="ACHOSC_RS22910"
                     /old_locus_tag="pgaptmp_004582"
     CDS             complement(<1..112)
                     /locus_tag="ACHOSC_RS22910"
                     /old_locus_tag="pgaptmp_004582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001325331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excisionase"
                     /protein_id="HGU4550373.1"
                     /translation="MYLTLQEWNARQRRPRSLETVRRWVRECRIFPPPVKD"
     gene            complement(219..563)
                     /locus_tag="ACHOSC_RS22915"
                     /old_locus_tag="pgaptmp_004583"
     CDS             complement(219..563)
                     /locus_tag="ACHOSC_RS22915"
                     /old_locus_tag="pgaptmp_004583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016248831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4550374.1"
                     /translation="MSDLSMKVLKWQSTGDVGISSATLASIACGLKKNIYGHHFGAPH
                     DAADFRRCVALVEKIPEIRDSFDKVAKRVPAFKGILNEWDSLVALLKSEMKIHGNKAP
                     ETYRRISELRKD"
     gene            complement(592..759)
                     /locus_tag="ACHOSC_RS22920"
                     /old_locus_tag="pgaptmp_004584"
     CDS             complement(592..759)
                     /locus_tag="ACHOSC_RS22920"
                     /old_locus_tag="pgaptmp_004584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4550375.1"
                     /translation="MHFRVTGEWNGEPFNRVIEAENINDCYDHWMIWAQIAHADVTNI
                     RIEELKEHQAA"
     gene            complement(832..1116)
                     /locus_tag="ACHOSC_RS22925"
                     /old_locus_tag="pgaptmp_004585"
     CDS             complement(832..1116)
                     /locus_tag="ACHOSC_RS22925"
                     /old_locus_tag="pgaptmp_004585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309190.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ASCH domain-containing protein"
                     /protein_id="HGU4550376.1"
                     /translation="MANLQLAVKGEYFDAMIRGEKTEEYRLCNDYWNKRIMFREYDRL
                     IITKGYPKRDDSSRRIDVPYDGYEIKTITHPHFGDKPVKVFAIKVNIGNE"
     gene            complement(1109..1252)
                     /locus_tag="ACHOSC_RS22930"
                     /old_locus_tag="pgaptmp_004586"
     CDS             complement(1109..1252)
                     /locus_tag="ACHOSC_RS22930"
                     /old_locus_tag="pgaptmp_004586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000870097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4550377.1"
                     /translation="MKVWVYDPETILNVEERRRTEVYIHNLNEDGFAPVLVKATPQEV
                     NRG"
     gene            complement(1262..1411)
                     /locus_tag="ACHOSC_RS22935"
                     /old_locus_tag="pgaptmp_004587"
                     /pseudo
     CDS             complement(1262..1411)
                     /locus_tag="ACHOSC_RS22935"
                     /old_locus_tag="pgaptmp_004587"
                     /inference="COORDINATES: protein motif:HMM:TIGR03655.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="Lar family restriction alleviation protein"
     gene            complement(1888..2025)
                     /locus_tag="ACHOSC_RS22940"
                     /old_locus_tag="pgaptmp_004588"
                     /pseudo
     CDS             complement(1888..2025)
                     /locus_tag="ACHOSC_RS22940"
                     /old_locus_tag="pgaptmp_004588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309978.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(2027..2584)
                     /locus_tag="ACHOSC_RS22945"
                     /old_locus_tag="pgaptmp_004589"
     CDS             complement(2027..2584)
                     /locus_tag="ACHOSC_RS22945"
                     /old_locus_tag="pgaptmp_004589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000034231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ead/Ea22-like family protein"
                     /protein_id="HGU4550378.1"
                     /translation="MSKIDYQALREAAERAIPAMERLLMLPVDDDLLTEQELKDYGVD
                     IDALNAFKFLTGPETVLALLDERERNQQYIKRRDQKNEDIALTVGKLRVELEAVQKTS
                     AARIEAIDRTHKMFQREKDRADAAEKCIAELSASHSKLRDTMAGIHNTIRMDGGYTPL
                     AAILNAAKRAYEESASAAGIRIKGE"
     gene            complement(2581..3138)
                     /locus_tag="ACHOSC_RS22950"
                     /old_locus_tag="pgaptmp_004590"
     CDS             complement(2581..3138)
                     /locus_tag="ACHOSC_RS22950"
                     /old_locus_tag="pgaptmp_004590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000812206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4550379.1"
                     /translation="MKQMTLIEMDGFLKGKCIPSDLKVDETNDEYLVRKFGELESKLE
                     TALRECRSAGITIDNLEAKCAKMAAENSSLKQSEKEFNDFCREEFSEWEDDITETPAT
                     DAFLAEVKTEARKEGAYFVANRMLAAWEAGFIDDTAKNAADIARMILTSTEFMANAPE
                     GDFDRSFSDGVLEDIAEQLRKGVIQ"
     gene            complement(3135..>3237)
                     /locus_tag="ACHOSC_RS22955"
                     /old_locus_tag="pgaptmp_004591"
     CDS             complement(3135..>3237)
                     /locus_tag="ACHOSC_RS22955"
                     /old_locus_tag="pgaptmp_004591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015980168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF2737 family protein"
                     /protein_id="HGU4550380.1"
                     /translation="KPMPSREELLKRKSFGSVNDNKYLNAMWRSGKK"
BASE COUNT          769 a          828 c          687 g          953 t
ORIGIN      
        1 catccttaac cggaggaggg aatatcctgc attcgcgcac ccatcgacga actgtttcaa
       61 ggcttcttgg gcgtcgctgg cgtgcattcc actcctgaag tgtcaagtac atcgcaaagt
      121 ctccgcaatt acacgcaaga aaagccgcat tgatgcggcg atggtaggtc tggatatcat
      181 tgagccatga acaggcctca tcgagtgtga ggctgtggtt agtcctttcg taactcgctg
      241 attctcctgt aagtctctgg tgctttgttt ccgtgtatct tcatttcaga cttcaacaga
      301 gcaacgaggg aatcccattc gttgaggatg cctttgaatg ccggaacgcg ctttgcaacc
      361 ttgtcgaatg aatctctgat ttctggaatc ttctcaacaa gtgcaacgca tcgtcggaag
      421 tctgctgcgt catggggagc gccgaagtga tgaccataga tattcttttt caggccacat
      481 gcgattgagg caagagttgc gctactgatg ccaacatcgc cagtcgattg ccatttcaaa
      541 accttcatag acaaatctga catttcttgt ctccaataaa aaaaacctcc atcaggcggc
      601 ttggtgttct ttcagttctt caattcgaat attggttacg tctgcatgtg ctatctgcgc
      661 ccatatcatc cagtggtcat agcagtcatt gatgttctct gcttcgataa ctctgttgaa
      721 tggttctcca ttccattcac ctgtgactcg gaagtgcatt tatcatctcc ataaaacaaa
      781 atccgccgta gcgagttcag ataaaagaaa tccccgcgag tgcgaggatt gttattcatt
      841 gccgatattc acctttatcg cgaacacctt taccggttta tcaccgaagt gcggatgtgt
      901 gattgtcttg atttcatatc cgtcatacgg aacatcaatt ctgcggctgg aatcgtcgcg
      961 cttcggatat ccctttgtga taatcaggcg gtcatactcc cggaacataa ttcgcttatt
     1021 ccaatagtca ttacacaggc gatactcttc cgttttctct cctcgaatca tggcatcgaa
     1081 gtattcacct ttgacggcaa gttgtaggtt agccacggtt aacctcctgc ggagtagcct
     1141 ttacaagcac tggtgcaaat ccatcttcat taaggttatg aatatagact tctgttctcc
     1201 tgcgctcttc aacgtttaat attgtttctg gatcataaac ccatactttc attcgactat
     1261 tccatgaaga aatcgcttca gatctagttc gtttttctgg tccttgggca ccacatttgc
     1321 atgacacata gcgcattttc cctctgatac tgaatgagta tccgatgtta agcacagtgg
     1381 aaccacagaa tgggcagcga tatagattca taaatcatcc accttaggcg cagctgagac
     1441 agcaacattc cagaactcag aaaacatatt gtatgcacca gatagattgg aagtagcata
     1501 ccctccaagc tcacttctaa tctgaacggc tctcatcatt tccggggtta actccttcgg
     1561 caccataacc caaccatccg gagttaccgg agagttgcca ccgggaatat tttccggaat
     1621 attttgttgt gcgttttgtg gttgttctga tttaccctga agcatggcgg cgcggcaggc
     1681 gttccagcca gctgttcgcc caagcgcgta aacttcagat ggatcaagat aatcaatgtc
     1741 atgcccgtcc tcatcgtcgt tctcaggtaa tgcagcaggt actaccggta ctggcggagc
     1801 ggcgtagacc tcaataattc cattatcaat aggccattct ccatccttga ggtagtcact
     1861 tgtgccgtca acttgctgtt ctgcaatgtg gaatgcacct attggttttg cttccagcga
     1921 tgccagagca atccgtgcca gttccatttg ttcgccacta gtaagtccgt tatcaagcgg
     1981 ggatttaatg aacaattcga tacgttcttt agtgatagtg ctcatatcac tctcctttga
     2041 tgcgaatgcc agctgcgctt gctgattctt catatgcgcg tttagcagcg ttaaggattg
     2101 ctgccagtgg cgtatagccg ccatccattc ggattgtgtt gtggatgcca gccattgtgt
     2161 cgcgcaattt gctgtggcta gcagacagtt ctgctatgca cttctctgcg gcatcggctc
     2221 gatctttttc acgctggaac attttgtgag tgcggtctat agcttcgatt cgtgccgctg
     2281 atgtcttctg cacggcttca agctcaacac gcagcttccc aaccgtaaga gcaatatcct
     2341 cgttcttctg gtcgcggcgt ttgatgtatt gctggtttct ttcccgttca tccagtagtg
     2401 ccagcacggt ttctggcccg gtcagaaatt tgaaggcgtt gagcgcatca atatccacac
     2461 cgtaatcctt aagttcctgt tcagttaaca aatcatcatc aactggcaac attaacaggc
     2521 gttccattgc cggaattgca cgctctgccg cctcacgcag tgcctgatag tcaatcttgc
     2581 tcattggatg actcctttac gaagctgttc ggcgatatct tcgagaacgc catcagagaa
     2641 tgaacggtca aaatcgcctt ccggcgcatt ggccataaac tcagtagagg taagaatcat
     2701 ccgggcaata tcagcggcgt tcttcgcagt atcatcaata aaaccagctt cccaggcagc
     2761 cagcattctg ttcgccacaa agtaagcgcc ctccttgcgt gcttcagtct tcacttcagc
     2821 cagaaaagca tcggtggctg gggtttcagt aatatcatct tcccattcgc taaactcctc
     2881 acgacaaaag tcattaaatt ccttctcaga ttgcttaagc gaggaatttt cagcagccat
     2941 cttcgcgcat ttagcctcaa ggttatcaat cgtgattcca gcagaacgac actcccgcaa
     3001 cgccgtttct agttttgatt caagttcacc gaacttacgg acaagatatt catcgtttgt
     3061 ttcgtcaacc tttaaatcac ttgggatgca tttacctttc agaaaaccat ccatctcaat
     3121 tagtgtcatt tgtttcattt cttcccactc cgccacatcg cattcagata tttgttgtca
     3181 ttaacagaac cgaaactctt tctcttaagc aattcctctc tcgatggcat tggcttt
//
LOCUS       DAZKHF020000353         3200 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2201S31241
            SAMN43267800-rid25106413.denovo.355, whole genome shotgun sequence.
ACCESSION   DAZKHF020000353 DAZKHF020000000
VERSION     DAZKHF020000353.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267800
            Sequence Read Archive: SRR30307263
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3200)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3200)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 3200)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350581.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350581.2
            Genome Coverage       :: 151x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:21:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,652
            CDSs (total)                      :: 5,557
            Genes (coding)                    :: 5,320
            CDSs (with protein)               :: 5,320
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 237
            CDSs (without protein)            :: 237
            Pseudo Genes (ambiguous residues) :: 0 of 237
            Pseudo Genes (frameshifted)       :: 102 of 237
            Pseudo Genes (incomplete)         :: 130 of 237
            Pseudo Genes (internal stop)      :: 52 of 237
            Pseudo Genes (multiple problems)  :: 41 of 237
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3200
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267800-rid25106413.denovo.355"
                     /serotype="O 26:H11"
                     /isolate="2201S31241"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-01"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            1..851
                     /locus_tag="ACHOSC_RS22960"
                     /old_locus_tag="pgaptmp_004592"
                     /pseudo
     CDS             1..851
                     /locus_tag="ACHOSC_RS22960"
                     /old_locus_tag="pgaptmp_004592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_462451.1"
                     /note="internal stop; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="phosphotriesterase-related protein"
     gene            complement(891..1183)
                     /locus_tag="ACHOSC_RS22965"
                     /old_locus_tag="pgaptmp_004593"
                     /pseudo
     CDS             complement(891..1183)
                     /locus_tag="ACHOSC_RS22965"
                     /old_locus_tag="pgaptmp_004593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709217.2"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing N-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyltransferase"
     gene            complement(1263..2884)
                     /locus_tag="ACHOSC_RS22970"
                     /old_locus_tag="pgaptmp_004594"
                     /pseudo
     CDS             complement(1263..2884)
                     /locus_tag="ACHOSC_RS22970"
                     /old_locus_tag="pgaptmp_004594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001368704.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
BASE COUNT          784 a          841 c          763 g          812 t
ORIGIN      
        1 cttattgctg cgctgataat atggaccgca aacctattga agatgtgatc ttcgaaatta
       61 ataactttat ctcgctcggt ggtcgcacca ttgttgatgc cacagggtct gagtctatcg
      121 gacgagatgc gcaggcatta cgagaagtcg cattaaaaac aggtctgaat attgttgctt
      181 cctccggtcc ctacctggaa aaatttgaaa gccagagaat tcataaaacg gttgatgaac
      241 tggcggcaac gattgataaa gaattgaatc aagggattgg cgatacggat attcgtgccg
      301 gaatgatcgg tgaaataggt gtctcaccga catttaccga agccgagcat aacagcttgc
      361 gggctgcctc gctggcacag attaacaatc ctcatgtggc gatgaatatt cacatgccgg
      421 gctggcttcg tcgcggtgat gaagtactcg acattgtgtt aggtgaaatg ggcgtctcgc
      481 caaataaagt ctctctcgca cactcggatc cgtcaggaaa agacgtggcg tatcagcgga
      541 aaatgcttga taagggtgtc tggctggaat tcgacatgat tggcctagac attaccttcc
      601 cgaaagaggg aatagcgcca ggggtgcagg agactgccga tgccgtcgct catctcattg
      661 agttgggata cgccgatcag cttgttctca gccacgatgt cttccttaaa caaatgtagg
      721 ctaaaaatgg cggtaatggc tggggatttg ttccagatgt ttttctggcc tatctggcgg
      781 agcgcggcgt cgataaaacg atcctcaaaa aactctgtat cgataatccc ggacgattat
      841 tgaccgcata aaaccattat cccccggcac actgcgccgg gggatgtcaa ttaatacccc
      901 gccgttaaat catccaccaa gcgcgagtcg gatgcgccgt acaactcacc atccgaccca
      961 accataatgc tttgcgtgct gcccatcgcc tcattcagcg ccactttctg accttttgct
     1021 tccagcagct tgagcgtatc cggactaaac ccttttcaac acgcagctcg tccggcaacc
     1081 actgatggtg gaaacgaggc gcattggtcg cttcggcaac attcattcca caattgatgc
     1141 tgttcaccac catttgcagc acggtagtga tgattcagct accggtaaca aagtgagtac
     1201 ataaaggcca tactgtcacg aacagtgcaa aagtggctct tccgcaacct aggtgaagat
     1261 tttcatgcca gtggactcat taccctccgc ctaagtagtt caaatccggc tcgaccatac
     1321 atctggcgta tcagcacctt caggcgattt acatgacctt cgacaacacc attactccag
     1381 cgactgctga ttgcctcaca tatagccgca gcatcagctt ccatccccgc agctacgcac
     1441 cgaagttctg ctgagccgct ttcgtggact cgagtgaacc agctgcttag ctgtgattta
     1501 ttttaggttt tcagaatacg gtagaactcg agtaccagtt gctgtgctat tttcaactcc
     1561 ggttcttttt cacacatcag actaataaat cgggatgcat aattttcttc gcccctgatt
     1621 attctccagg gcatcaaccc gcggcttacc cggaacactg aaggaagtct tgcggcagta
     1681 gtaaccggtg ggttccagcc tttacgccat ttggcaacag tatccctgac gatggtttca
     1741 ctgcctgtcc ccccccctgg gccaccattt cccgccatat ccggctggca ttataattac
     1801 cactttctcg ttgctctttt aaccactccc tccatgggtc cagaagccct cgctttggag
     1861 gccgggttga catttcaggg aatgttcctg aacgcaccca gcgactgact gtcactcgcg
     1921 acaggcctgt aatacgggat atttccctga aactacatcc cttgttatgc agggccataa
     1981 cctctgtcca atgctgatgc cgttttttgc gggtttgctg tttaaggcgt tccggacgac
     2041 ggagcgatgc tacaggcaca gatatttctg gctcaggtga tttcttcagt gataactctc
     2101 tgacaacaag acgtatcaga ggcatatgtc tgtacatcat tcgttcaggc tcatcaccaa
     2161 tattttttag caggtgccat cgatcggcca cctgtctggc ctgaggtgca ccttcacgtg
     2221 ctgctgtcgc atagactcca ctgcgatcac gcgagacaac ctgtatttcc ggatattttc
     2281 tgaaccaggt cgccagcgta cgctgatcac ggccgggaag caggacgagg ggacggtgag
     2341 tatcaagatt gactattaac ataccgcagc agtggccccg atgccacgcc cactcatcaa
     2401 taccgacatg aggcgcgcct gactgtttcg tctccggggt attgataacc ctgcgaagaa
     2461 gagtatcagc actgcaacgg agacccgctg ccgttgcagc ccgttttcca gcctcacctc
     2521 cagctattaa tcccagttga cgttgtaaat tttgtaacgc ctgtgaagac tgttggtgtg
     2581 aaccggcgaa gggagcaagc gactcggcaa aaattttacg tgaacaagct gggttacggc
     2641 agtaccagtg gcggacggaa aatacaagcc atagcgtctg tcctgatcag ggtagatgct
     2701 gtatccggcg ccgacaacaa ctatgaacag agtcgctacg cttaaagcat tcaggacatt
     2761 gtgctgtttt gcgttttccg tggagatgga gggttatacc gtcagagcta ataatttgtc
     2821 gactgcatcg ccatccgcaa ggtaactgta gaagtgtctt aagagagtgc attgggttgc
     2881 ccataactta aaacagctta gatctttgtt cgccagaaag tttcatccat tttcttccac
     2941 ccaggttgcg gaagaaccac ttttcatccg tcgttgacag atgtgaagta tgcgggagaa
     3001 aaagtgaata tctaactgta ataactcaga ccggtacgag tactcggggc gtctacaatc
     3061 attcagggca aagtgctaaa atttctctat cccgggatgc ccctaccaac gcagcactcc
     3121 cggagatatc attacagctc tgcaactgat ttccgcgact gaaccgctca gttcctatcc
     3181 ttccagttgc cttcctgcag
//
LOCUS       DAZKHF020000354         3197 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2201S31241
            SAMN43267800-rid25106413.denovo.356, whole genome shotgun sequence.
ACCESSION   DAZKHF020000354 DAZKHF020000000
VERSION     DAZKHF020000354.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267800
            Sequence Read Archive: SRR30307263
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3197)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3197)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 3197)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350581.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350581.2
            Genome Coverage       :: 151x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:21:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,652
            CDSs (total)                      :: 5,557
            Genes (coding)                    :: 5,320
            CDSs (with protein)               :: 5,320
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 237
            CDSs (without protein)            :: 237
            Pseudo Genes (ambiguous residues) :: 0 of 237
            Pseudo Genes (frameshifted)       :: 102 of 237
            Pseudo Genes (incomplete)         :: 130 of 237
            Pseudo Genes (internal stop)      :: 52 of 237
            Pseudo Genes (multiple problems)  :: 41 of 237
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3197
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267800-rid25106413.denovo.356"
                     /serotype="O 26:H11"
                     /isolate="2201S31241"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-01"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            complement(90..872)
                     /locus_tag="ACHOSC_RS22975"
                     /old_locus_tag="pgaptmp_004595"
     CDS             complement(90..872)
                     /locus_tag="ACHOSC_RS22975"
                     /old_locus_tag="pgaptmp_004595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001164193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HGU4550381.1"
                     /translation="MQHLPAPIHHARDAAQLPVAIDYPAALALRQMSMVHDELPKYLL
                     APEVSALLHYVPDLRRKMLLATLWNTGARINEALALTRGDFSLTPPYPFVQLATLKQR
                     TEKAARTAGRTPAGQQTHRLVPLSDSWYVSQLQTMVATLKIPMERRNKRTGRTEKARI
                     WEVTDRTVRTWIGEAVAAAAADGVTFSVPVTPHTFRHSYAMHMLYAGIPLKVLQSLMG
                     HKSISSTEVYTKVFALDVAARHRVQFSMPESDAVTMLKNKHA"
     gene            complement(874..1287)
                     /locus_tag="ACHOSC_RS22980"
                     /old_locus_tag="pgaptmp_004596"
     CDS             complement(874..1287)
                     /locus_tag="ACHOSC_RS22980"
                     /old_locus_tag="pgaptmp_004596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001536595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4550382.1"
                     /translation="MFFIENEGQAVARTDYWQSVQAQAGYVYLSWNAGAARLLVPDAA
                     KHLLREMRGAEYVIISKGTLHGRDALELIFEDGSDAPFVIHMLSEQCDRLLPENNQGG
                     GFVVTVWTRGGNQLRYPGKYRVVENLPDVSPWSEH"
     gene            1847..2077
                     /gene="vapB"
                     /locus_tag="ACHOSC_RS22985"
                     /old_locus_tag="pgaptmp_004597"
     CDS             1847..2077
                     /gene="vapB"
                     /locus_tag="ACHOSC_RS22985"
                     /old_locus_tag="pgaptmp_004597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005229629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system VapB family
                     antitoxin"
                     /protein_id="HGU4550383.1"
                     /translation="MRTVSIFKNGNNRAIRLPRDLDFEGVSELEIVREGDSIILRPVR
                     PTWGSFLEYEKADPDFMAEREDVVSDEGRFNL"
     gene            2074..2490
                     /locus_tag="ACHOSC_RS22990"
                     /old_locus_tag="pgaptmp_004598"
     CDS             2074..2490
                     /locus_tag="ACHOSC_RS22990"
                     /old_locus_tag="pgaptmp_004598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005229630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system VapC family
                     toxin"
                     /protein_id="HGU4550384.1"
                     /translation="MNKTYMLDTCICSFIMREQPEAVLKRLEQAVLRGHRIVVSAITY
                     SEMRFGATGPKASPRHVQLVDKFCARLDAILPWDRAAVDATTKIKVALRLAGTPIGPN
                     DTAIAGHAIAAGAILVTNNTREFERVPDLVLEDWVK"
     gene            complement(2652..>3197)
                     /locus_tag="ACHOSC_RS22995"
                     /old_locus_tag="pgaptmp_004599"
     CDS             complement(2652..>3197)
                     /locus_tag="ACHOSC_RS22995"
                     /old_locus_tag="pgaptmp_004599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000350636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4550385.1"
                     /translation="MQICKSITASADCEEIIKLIYLRRYFEIVDERGDAYQLLSNLFH
                     RRVAPLDYREPAAAGSGYPKMAPEKIQQALRDIREYVDSFDYPRLQALVSSPDEIKNL
                     YRRCRNGYEKLQVFRLLELDQDHPVIRKFVNETYHIENEFICQLDPSRFDLIPEYVIM
                     ECDKLIALPPAANQSSVARIA"
BASE COUNT          766 a          853 c          844 g          734 t
ORIGIN      
        1 gctttaacaa ttatgaaata atacaagaga tcacatattg cagtggtctc ttttttatag
       61 aagaaatcac aattcataat tgtgacttat tatgcatgtt tgtttttcag cattgtgacc
      121 gcatcggact caggcatcga aaactgcacc cggtgccggg cagcgacatc cagcgcaaac
      181 accttcgtgt agacctccgt tgagctgatg gacttatgcc ccatcagact ctgcaggacc
      241 ttcagcggta taccggcata cagcatatgc atcgcatagg aatggcggaa cgtatgtggc
      301 gtgaccggga cagagaacgt cacaccatca gcggcagcgg cggcaaccgc ctccccaatc
      361 caggtcctga ccgttctgtc cgtcacttcc cagatccgcg ctttctctgt cctgcctgtt
      421 cgtttattac gccgttccat ggggattttc aatgtggcca ccatcgtctg cagctggctg
      481 acgtaccagg agtcggagag cggaaccagc cggtgagtct gctgcccggc gggcgttctt
      541 cctgccgtcc tggcggcttt ttcggtccgt tgtttcaggg tcgcaagctg cacaaacgga
      601 tacggaggcg tgagcgaaaa atccccccgc gtcagcgcca gtgcttcatt aatgcgcgca
      661 ccggtgttcc acagtgtggc cagcagcatc ttgcggcgca gatccgggac gtaatggagc
      721 agggcgctca cttcaggggc cagcaggtat ttgggcagtt catcatgaac catcgacatc
      781 tggcggagtg ccagcgctgc cggataatcg atggcaacag gaagctgagc ggcatcccgg
      841 gcatggtgga tcggtgccgg caggtgctgc atatcagtgt tcactccacg gggaaacgtc
      901 gggcaggttt tccacaaccc ggtactttcc cggataacgg agctggttac cgccacgcgt
      961 ccagacggtg acaacaaaac cccctccctg gttgttttcg gggagcaggc gatcgcactg
     1021 ctcactcagc atgtggatca cgaacggcgc atcgctgccg tcttcaaata tcagttccag
     1081 cgcatcgcgg ccatgcagcg ttcccttact gatgatgacg tactcagccc cccgcatctc
     1141 cctgagtaaa tgttttgccg catccgggac aagcagcctg gctgcgccgg cattccagct
     1201 gaggtagaca tatccggcct gcgcctgtac agactgccag taatccgttc tggcgacagc
     1261 ctgaccttca ttctcaataa aaaacatggg tgactccttt tttcttttcc gggcagggac
     1321 ggtcatgcga taacgtcatc catcatcact gctcttaccg tcacgccatg tgataacatc
     1381 atgccgttat catcatcacc atgataacaa gcatccatat aaccacgctg ttaaattacc
     1441 ggaggaccat ttccgacaga tccgattttt ctgttattga cgggttttcg gaagtgtatc
     1501 acgctgagga aataatcata tcgctgtttt ccgcccctgc gcgacgcatc acaggtcagg
     1561 gagatgacac aacatatagt gtcatcagca gatgaatccg gcaccacatg ttgtgatgac
     1621 gggaggtggc gttgtgtggg gggagaggaa gatcctgaac aacctttttt cggatctccg
     1681 ataaaaaaga aagaaataga gatatgctaa acatatgcct ggcatctaca tatgctaccg
     1741 ctatacatat gccacacatc tgcctatact aaacatatgc acacacccgg tgctttgaat
     1801 attccggata tgtttcacat atccatatgc tcgggagaga aagaacatga gaaccgtatc
     1861 gatatttaaa aatggcaaca accgcgccat ccgtctgcct cgtgatctgg attttgaggg
     1921 ggtgagcgag ctcgagatcg tccgggaagg ggacagcatc atcctgcgcc ccgtccggcc
     1981 aacctgggga tcattcctag agtacgaaaa agccgatcca gattttatgg cggagcgcga
     2041 ggatgttgtc agcgacgaag ggcgatttaa cctgtgaaca agacttatat gcttgatacc
     2101 tgtatctgct cgttcatcat gcgtgaacag ccagaagccg tcctgaagcg tctggagcag
     2161 gcggtgctac gcggccaccg catagtggtc tcggccatca cctactctga aatgcgcttc
     2221 ggggctaccg gcccaaaggc ctcaccacgt cacgtccaac tggtcgataa attctgcgcc
     2281 cgcctcgatg ccatcctgcc ctgggatcgc gccgcagtag acgccaccac gaagattaaa
     2341 gtggcactgc ggctggccgg gacgccgatc ggcccgaacg acacggcgat tgccgggcac
     2401 gccatcgccg ccggggcgat actggtaacg aataatacaa gagaatttga gcgggtgcct
     2461 gacctggtgc tggaagactg ggtgaagtaa gacactcttt tcaaagtcag gagataaggg
     2521 caccaagaaa cagcccgtat acgggctgtt ttaacagata tctacagtaa gcggtcctaa
     2581 actgtagggt atcttcgaag atcagactat cagggagcta aagcctatct tccggcggca
     2641 aaaacagtgg ctcaagcgat acgggcaacc gagctctggt ttgctgccgg tggcagggcg
     2701 atgagcttgt cacattccat aatcacgtac tccgggatga gatcgaatct ggacggatct
     2761 agttgacaga tgaactcatt ttcaatgtgg tacgtttcgt taacgaattt tcgtattacc
     2821 ggatggtcct gatccagttc cagtaaacgg aatacctgca gtttttcata gccgttacga
     2881 cagcgacggt acaggttttt tatttcatct gggctgctga cgagagcctg aagtcgcggg
     2941 taatcaaaac tgtccacata ctcccggata tcccgcaagg cctgctgaat tttctcagga
     3001 gccattttgg ggtagcccga gcccgcagcg gcaggctcgc gataatccag cggggcgacg
     3061 cgtcgatgga atagattgga tagaagctga taagcatcac cacgttcgtc aacgatttca
     3121 aagtagcgac gaagatagat gagcttaatg atctcctcac agtctgctga cgcagtgatg
     3181 gatttgcaga tctgcat
//
LOCUS       DAZKHF020000355         3188 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2201S31241
            SAMN43267800-rid25106413.denovo.357, whole genome shotgun sequence.
ACCESSION   DAZKHF020000355 DAZKHF020000000
VERSION     DAZKHF020000355.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267800
            Sequence Read Archive: SRR30307263
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3188)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3188)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 3188)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350581.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350581.2
            Genome Coverage       :: 151x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:21:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,652
            CDSs (total)                      :: 5,557
            Genes (coding)                    :: 5,320
            CDSs (with protein)               :: 5,320
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 237
            CDSs (without protein)            :: 237
            Pseudo Genes (ambiguous residues) :: 0 of 237
            Pseudo Genes (frameshifted)       :: 102 of 237
            Pseudo Genes (incomplete)         :: 130 of 237
            Pseudo Genes (internal stop)      :: 52 of 237
            Pseudo Genes (multiple problems)  :: 41 of 237
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3188
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267800-rid25106413.denovo.357"
                     /serotype="O 26:H11"
                     /isolate="2201S31241"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-01"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            158..373
                     /locus_tag="ACHOSC_RS23000"
                     /old_locus_tag="pgaptmp_004600"
     CDS             158..373
                     /locus_tag="ACHOSC_RS23000"
                     /old_locus_tag="pgaptmp_004600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0020002 - host cell plasma membrane
                     [Evidence IEA];
                     GO_function: GO:0042802 - identical protein binding
                     [Evidence IEA];
                     GO_function: GO:0140911 - pore-forming activity [Evidence
                     IEA];
                     GO_process: GO:0019835 - cytolysis [Evidence IEA];
                     GO_process: GO:0044659 - viral release from host cell by
                     cytolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class II holin family protein"
                     /protein_id="HGU4550386.1"
                     /translation="MYQMEKITTGVSYTTSAVGTGYWLLQLLDKVSPSQWVAIGVLGS
                     LLFGLLTYLTNLYFKIREDRRKTARGD"
     gene            378..722
                     /locus_tag="ACHOSC_RS23005"
                     /old_locus_tag="pgaptmp_004601"
     CDS             378..722
                     /locus_tag="ACHOSC_RS23005"
                     /old_locus_tag="pgaptmp_004601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdfR family protein"
                     /protein_id="HGU4550387.1"
                     /translation="MKKKYELVVKEINNYPDKIAVTVALEIGGHPSLLLPHVAISLDR
                     TEGATLEFYEAEAKKQAKQFFMDVAAGLCEGDGPLPEKRPVILEAQDVLITYRGKLPG
                     IITGSLKTPPLA"
     gene            773..1306
                     /locus_tag="ACHOSC_RS23010"
                     /old_locus_tag="pgaptmp_004602"
     CDS             773..1306
                     /locus_tag="ACHOSC_RS23010"
                     /old_locus_tag="pgaptmp_004602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysozyme"
                     /protein_id="HGU4550388.1"
                     /translation="MNAKIRYGLSAAVLALIAAGAPAPEILDQFLDEKEGNHTTAYRD
                     GAGIWTICRGATRVDGKPVIPGMKLSKEKCDRVNAIERDKALAWVEKNIRVPLTEPQK
                     AGIASFCPYNIGPGKCFPSTFYRRINAGDRKGACEAIRWWIKDGGRDCRIRSNNCYGQ
                     VSRRDQESALACWGIDR"
     gene            1577..2143
                     /locus_tag="ACHOSC_RS23015"
                     /old_locus_tag="pgaptmp_004603"
     CDS             1577..2143
                     /locus_tag="ACHOSC_RS23015"
                     /old_locus_tag="pgaptmp_004603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage antirepressor N-terminal domain-containing
                     protein"
                     /protein_id="HGU4550389.1"
                     /translation="MNMMAVPFHGNSLYVVNHNGEPYVPMKPVVAGMGLAWQSQLAKL
                     RQRFASTITEIVMVAEDGKQRNMVSMPLRKLAGWLQTINPNKVKPEIRDKVIRYQEEC
                     DDVLYEYWTKGFVVNPRKMSVMEELNQACADMKRDKNIASVFATGLNEWKQVKAAHVS
                     KIRTLVNEANMIDFVLADTGKGKITKAD"
     gene            2143..2289
                     /locus_tag="ACHOSC_RS23020"
                     /old_locus_tag="pgaptmp_004604"
     CDS             2143..2289
                     /locus_tag="ACHOSC_RS23020"
                     /old_locus_tag="pgaptmp_004604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4550390.1"
                     /translation="MGWLMISDKLITLVKSLCVLVGISFLVMLVAIFFSTAWRVLTLS
                     GLVG"
     gene            2517..2723
                     /locus_tag="ACHOSC_RS23025"
                     /old_locus_tag="pgaptmp_004605"
     CDS             2517..2723
                     /locus_tag="ACHOSC_RS23025"
                     /old_locus_tag="pgaptmp_004605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rz1 family lipoprotein"
                     /protein_id="HGU4550391.1"
                     /translation="MRELKMKLCVLMLPLVVSACGSTPPAPVPCVKPPAPPAWAMMPP
                     SNSLRLLDETFSVSETESSATRQH"
     gene            2788..3012
                     /locus_tag="ACHOSC_RS23030"
                     /old_locus_tag="pgaptmp_004606"
     CDS             2788..3012
                     /locus_tag="ACHOSC_RS23030"
                     /old_locus_tag="pgaptmp_004606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4550392.1"
                     /translation="MKKLLVTVKPFQGTIPFRILQRGRVLVEGSFSGKCTQLHSRTFQ
                     VNATNEELTVECTMNAAKCRMVSAALQPVC"
BASE COUNT          831 a          698 c          887 g          772 t
ORIGIN      
        1 catcataaaa agtgagccag tcaaatggaa ggtatcgtta aactcaccgg tagtgtcagt
       61 gggtcgtctg agacgcctgc atgagttatc agagccatca gtagttaact ggtggctttt
      121 ttattgttgt cagcttccgg ataacgggag acggggtatg taccagatgg aaaaaatcac
      181 aacaggtgtg tcatacacca cgtcagcggt agggacggga tactggttac tgcaactgct
      241 ggacaaagtc tctccgtccc agtgggtggc aataggtgta ctggggagtc tgctgtttgg
      301 cctgctgacg tatctgacta acctgtattt caaaatcagg gaggaccgcc gtaagacggc
      361 gcggggagac taaagcgatg aagaaaaaat acgaactggt tgttaaagag ataaataatt
      421 acccggataa gattgctgtt actgtggcac ttgaaattgg cgggcatccg tcgttgttgt
      481 tgccacatgt ggcgattagt cttgaccgta ctgaaggtgc cacgctggag ttttacgaag
      541 ctgaggcgaa aaagcaggcg aagcagtttt tcatggatgt tgctgccggg ttatgtgaag
      601 gggatggtcc gttaccggaa aagcgtcccg taattttaga ggcgcaggat gtgttgataa
      661 cctacagagg aaaactaccg ggaataatta cgggttctct gaagactcca ccgctggcct
      721 gaagacttaa catatccagg gatttgaaat cgataaaccc tgataaatat ccatgaacgc
      781 aaaaatcaga tacggcctgt cggctgccgt tctggcgctg attgccgcag gtgcgcctgc
      841 gcctgaaatc ctcgaccagt ttctggatga aaaggaaggt aaccacacca cagcataccg
      901 tgatggtgcg ggtatctgga ccatctgccg tggagccacc cgggtggatg gtaagcctgt
      961 tattcctggc atgaagctgt cgaaggaaaa atgcgaccgg gttaacgcca ttgagcgtga
     1021 taaggcgctg gcatgggtgg agaaaaacat cagagtgcca ctgaccgaac cccagaaagc
     1081 ggggattgcg tcattctgtc cgtacaacat tggccccggt aagtgtttcc cgtcgacgtt
     1141 ttacagacgg attaatgcag gagatcgaaa aggtgcctgc gaagctattc gctggtggat
     1201 taaggacggt ggcagggact gccgtattcg ctcaaataac tgttacggtc aggtatcccg
     1261 tcgtgaccag gagagcgcgc tggcgtgctg gggaatcgac agataagcag aatattttgc
     1321 tgaaaaatga ggtttgctta catggatgga taacacgaaa tcctgcaaat tggcaaaatg
     1381 taagtgaata aagtcaaaac agttgtttaa cactcaggca ccgtaatgat gcctttgtca
     1441 tttctgcgca tctcacgcgc atctcacaac acagaacctt tcaggatgac ccttgaggat
     1501 accggtttgg ctgtcggtgc ctttctgtgg gctggattcc tgtgagacaa ggttcatcac
     1561 taaaaggaaa taaccgatga atatgatggc cgtgccgttt cacggcaact ctctttatgt
     1621 agttaaccat aatggcgaac catacgttcc catgaaacct gtcgttgcgg ggatggggct
     1681 ggcctggcaa tcacagttgg ctaagttaag acagcgtttt gcgtcaacta taacggaaat
     1741 cgttatggtt gctgaggatg ggaaacaacg caatatggtg tccatgccac ttcgaaaact
     1801 tgccggctgg ctacaaacca ttaatcccaa caaagtaaaa cccgaaatcc gcgataaggt
     1861 catccggtat caggaagagt gcgacgatgt tctttacgag tactggacga agggttttgt
     1921 cgttaatccc cgtaaaatga gcgtgatgga agaactcaac caggcttgtg ctgacatgaa
     1981 acgggataaa aacattgcca gtgtgtttgc taccgggctg aatgagtgga aacaggttaa
     2041 agccgcgcat gtatcaaaaa tccgtacgct ggtaaatgaa gcgaatatga ttgattttgt
     2101 cctggctgat acaggcaaag ggaaaataac aaaggcggat tgatggggtg gctaatgata
     2161 tcagataaac tcataacgct ggtgaagagc ctctgtgtac ttgtcggcat ttcattttta
     2221 gtcatgctgg ttgccatttt cttttccacc gcctggcgag tcctgacgtt atcgggactg
     2281 gtggggtgaa agagagatga accgtgttct gtgtgtggtg attattgtca tggcggttgg
     2341 ctgtggtgcg ctgtggctgg caacaaacca ttaccgtgac aacgcgctca cctacaaagc
     2401 gcagcgcgat aaaaaagcca gagagctgga acaggcgaat gccaccatta ctgacatgca
     2461 ggtgcgccag cgtgatgttg ctgcgctcga tgcaaaatac tcgagggaat tagccgatgc
     2521 gagagctgaa aatgaaactc tgcgtgctga tgttgccgct ggtcgtaagc gcctgcggat
     2581 caacgccacc tgccccggta ccgtgcgtga agccaccggc acctccggcg tgggcaatga
     2641 tgccgccgtc gaactctctc cggttgctgg acgaaacgtt ctcggtatca gagacggaat
     2701 catcagcgac caggcagcat tgagaatgct tcaggaatat atccgcactc agtgtattaa
     2761 ctagtatttt tgttattcgg agaatgcatg aagaaattat tggtaaccgt aaagcctttt
     2821 cagggaacaa ttccgttccg tattttgcag cgtggtcgtg ttcttgttga aggttcgttc
     2881 agtggtaaat gtacgcaatt acactccagg acctttcagg tgaatgccac gaatgaagag
     2941 ctaaccgttg agtgtacgat gaatgccgct aaatgccgca tggtatccgc tgcattacag
     3001 ccagtgtgtt gagcgacctt attattcatg cgcggtattg tcgccgtatt cctgcattaa
     3061 cagagaccgc agcccgaccg ggagaatcct ctgcgcgagt gtgcggggat aatcaaaaac
     3121 gatacacacc ggggtttacc gcgttaacgg agcgcggcgt tgtcccctca tggtcgctgg
     3181 tccggtgc
//
LOCUS       DAZKHF020000356         3138 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2201S31241
            SAMN43267800-rid25106413.denovo.358, whole genome shotgun sequence.
ACCESSION   DAZKHF020000356 DAZKHF020000000
VERSION     DAZKHF020000356.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267800
            Sequence Read Archive: SRR30307263
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3138)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3138)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 3138)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350581.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350581.2
            Genome Coverage       :: 151x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:21:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,652
            CDSs (total)                      :: 5,557
            Genes (coding)                    :: 5,320
            CDSs (with protein)               :: 5,320
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 237
            CDSs (without protein)            :: 237
            Pseudo Genes (ambiguous residues) :: 0 of 237
            Pseudo Genes (frameshifted)       :: 102 of 237
            Pseudo Genes (incomplete)         :: 130 of 237
            Pseudo Genes (internal stop)      :: 52 of 237
            Pseudo Genes (multiple problems)  :: 41 of 237
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3138
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267800-rid25106413.denovo.358"
                     /serotype="O 26:H11"
                     /isolate="2201S31241"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-01"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            101..421
                     /locus_tag="ACHOSC_RS23035"
                     /old_locus_tag="pgaptmp_004607"
     CDS             101..421
                     /locus_tag="ACHOSC_RS23035"
                     /old_locus_tag="pgaptmp_004607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005224852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5375 domain-containing protein"
                     /protein_id="HGU4550393.1"
                     /translation="MKTPLPPVLRAALYRRAVACAWLTVCERQHRYPHLTLESLEAAI
                     AAELEGFYLRQHGEEKGRQIACALLEDLMESGPLKAAPSLSFLGLVVMDELCARHIKA
                     PVLH"
     gene            436..2769
                     /locus_tag="ACHOSC_RS23040"
                     /old_locus_tag="pgaptmp_004608"
     CDS             436..2769
                     /locus_tag="ACHOSC_RS23040"
                     /old_locus_tag="pgaptmp_004608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000783678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003896 - DNA primase activity [Evidence
                     IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="primase-helicase zinc-binding domain-containing
                     protein"
                     /protein_id="HGU4550394.1"
                     /translation="MKMNVTATVSHALGHWPRILPALGIQVLKNHHQPCPVCGGSDRF
                     RFDDREGRGTWYCNQCGAGDGLKLVEKVFGVSPSDAAAKVAAVTGSLPPADPAVTAAA
                     VAETDAARKNAAALAQTLMAKTRPGTGNAYLTRKGFPGRECRMLTGTHRAGGVSWRAG
                     DLVVPLYDDSGELVNLQLISADGRKRTLKGGQVRGTCHTLEGQNQAGKRLWIAEGYAT
                     ALTVHHLTGETVMVALSSVNLLSLASLARQKHPACQIVLAADRDLSGDGQKKAAAAAD
                     ACEGVVALPPVFGDWNDAFTQYGGEATRKAIYDAIRPPAESPFDTMSEAEFSAMSTSE
                     KAMRIYEHYGEALAVDANGQLLSRYENGVWKVLPPQDFARDVAGLFQRLRAPFSSGKV
                     ASVVDTLKLIIPQQEAPSRHLIGFRNGVLDTQNGTFHPHSPSHWMRTLCDVDFTPPVD
                     GETLETHAPAFWRWLDRAAGGRAEKRDVILAALFMVLANRYDWQLFLEVTGPGGSGKS
                     IMAEIATLLAGEDNATSATIETLESPRERAALTGFSLIRLPDQEKWSGDGAGLKAITG
                     GDAVSVDPKYRDAYSTHIPAVILAVNNNPMRFTDRSGGVSRRRVIIHFPEQIAPQERD
                     PQLKDKITRELAVIVRHLMQKFSDPMLARSLLQSQQNSDEALNIKRDADPTFDFIGYL
                     ETLPQTSGMYMGNASIIPRNYRKYLYHAYLAYMEANGYRNVLSLKMFGLGLPVMLKEY
                     GLNYEKRHTKQGIQTNLTLKEESYGDWLPKCDDPATT"
BASE COUNT          709 a          924 c          897 g          608 t
ORIGIN      
        1 ttcgcgcagg gatacgcacg tcttcaggag acgcagatat gccttatcag ttaatgcaac
       61 cggcacggaa tgcagtcatc tgtcacaggg aggaaagcaa atgaaaacac ccttaccgcc
      121 cgtcttacgc gctgcccttt accgtcgcgc tgtcgcctgt gcctggctga ccgtgtgcga
      181 acgtcagcac cgctacccgc atctcaccct tgagtcactg gaggcggcca tcgccgctga
      241 gctggaaggc ttttatctgc gccagcacgg tgaggaaaaa gggcgtcaga tagcctgtgc
      301 cctgctggaa gatttaatgg aatccggccc cctgaaggcc gcgccgtcgc tgtcctttct
      361 cgggctggtt gtgatggatg aactctgtgc ccgtcacata aaagcgccgg tactgcactg
      421 aaggagaaca acaccatgaa aatgaacgta accgccaccg tcagccatgc gctcggccac
      481 tggccgcgta ttctcccggc gctggggatt caggtgctga agaaccatca tcagccctgt
      541 ccggtctgtg gcgggagtga ccgcttccgt tttgatgaca gggaggggcg cggcacctgg
      601 tactgcaatc agtgtggtgc cggtgacggc ctgaaactgg ttgaaaaggt gtttggtgtt
      661 tccccgtccg acgcggcagc aaaggtggct gccgtgaccg gcagcctgcc cccggctgac
      721 ccggcagtga cggccgccgc cgttgctgaa acagacgctg cccggaagaa cgccgccgca
      781 ctggcacaaa ccctgatggc gaaaacccgt cccggaaccg gtaacgccta cctgacccgc
      841 aagggctttc ccggccggga atgccggatg ctgaccggca cacacagagc cggtggcgtg
      901 agctggcgcg ccggtgacct tgtggtgcca ctgtatgacg acagcggcga actggttaac
      961 cttcagttaa tcagtgctga cggccgtaag cgcaccctga aaggcggaca ggtcaggggc
     1021 acctgtcaca cccttgaagg acagaatcag gccggaaaac gtctgtggat agcggaggga
     1081 tacgcgaccg cacttaccgt gcatcacctg accggtgaaa cggtaatggt ggcgctttct
     1141 tccgtgaacc tcctttctct ggccagcctt gcccggcaga agcatccggc ctgtcagatt
     1201 gtccttgccg cagaccgtga cctcagcggt gacggccaga aaaaagccgc cgcagccgca
     1261 gatgcgtgtg aaggtgttgt tgccctgccg ccggtcttcg gtgactggaa tgatgccttc
     1321 acgcagtacg gcggggaagc cacccgtaag gccatttatg atgccatccg gccaccggct
     1381 gaaagcccgt tcgacaccat gagcgaagcg gagttctccg ccatgagtac cagcgaaaag
     1441 gccatgcgta tctatgagca ttacggcgag gcgctcgcgg ttgatgccaa cggccagctt
     1501 ctgtcccgtt atgaaaatgg tgtctggaag gtgctgccac cacaggactt tgcccgggat
     1561 gtggccgggc tgtttcagcg gctgcgcgcg ccgttctcct ccgggaaggt ggcctccgtg
     1621 gttgacaccc tgaagctgat tattccgcag caggaagccc catcccgcca cctgattggc
     1681 tttcgtaacg gcgtgctcga cacgcagaac ggcacgttcc acccgcacag tccgtcacac
     1741 tggatgcgca ccctgtgcga tgtggatttc accccgccgg tggacggtga aacgctggaa
     1801 acccacgctc ccgcgttctg gcgctggctt gaccgtgccg ccggtggccg tgcggaaaaa
     1861 cgcgacgtga ttctggctgc actgtttatg gtgctggcaa accgctacga ctggcagctc
     1921 tttctggagg tgaccggtcc cggcggcagc ggcaaaagta tcatggccga aatagccacc
     1981 ctgctggccg gggaggataa cgccacgtcg gccaccatcg agacgctgga atccccgcgt
     2041 gaacgtgccg cgttaactgg cttctcactg atacgcctgc cggatcagga aaaatggagc
     2101 ggcgacggtg ccggactcaa ggccatcacc ggcggcgatg cggtgtccgt ggacccgaaa
     2161 taccgggatg cgtactccac gcatattccg gcggtaattc tggccgtgaa caataacccg
     2221 atgcgcttca ccgaccgcag cggcggcgtg tcacgcaggc gggtgattat tcacttcccg
     2281 gaacagatag ccccgcagga gcgcgacccg cagcttaagg acaaaatcac ccgcgagctg
     2341 gcggtcatcg tgcgtcacct gatgcagaag ttcagcgacc cgatgctcgc ccggtcactg
     2401 cttcagtccc agcagaactc agacgaggca ttgaacatca aacgggatgc cgacccgacg
     2461 tttgatttta tcggctatct ggaaaccctg ccgcagacca gcggcatgta tatggggaac
     2521 gccagtatca tcccgcgtaa ttaccgtaaa tacctctatc acgcctatct ggcctacatg
     2581 gaggcaaacg gctaccggaa tgtactcagt ctgaaaatgt tcgggctggg gctgccggtg
     2641 atgctgaagg aatacggact gaattacgag aagcgccata ccaaacaggg gatacagacc
     2701 aacctgacgc tgaaagagga aagctacggc gactggctgc caaaatgtga cgaccctgca
     2761 acaacctgac cctcatgact catctgaccg gcatctgccg gtcttttttc atccctgaat
     2821 cccccgaagg tgaacaatcc actgttcacc cttcaccgta tattcacccg ttatcacact
     2881 gaaattaaaa gagaaaaacg aaaggtgaac agtgtgaaca atcaaatcaa aaaaaacttt
     2941 tttcttcctg agtgatttca gtgcggagga ttaatcaccg gtatgagtca caccggcaga
     3001 atgccggagg tgaagaatcg aatgttcacc cttcacccat tattcaccac ctatcaaact
     3061 gaaataaaag gagaaaacag aaaggtgaac agtgtgaaca gttcttccga aaaaaaattt
     3121 ttttcctgag ttaacgtg
//
LOCUS       DAZKHF020000357         3136 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2201S31241
            SAMN43267800-rid25106413.denovo.359, whole genome shotgun sequence.
ACCESSION   DAZKHF020000357 DAZKHF020000000
VERSION     DAZKHF020000357.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267800
            Sequence Read Archive: SRR30307263
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3136)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3136)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 3136)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350581.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350581.2
            Genome Coverage       :: 151x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:21:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,652
            CDSs (total)                      :: 5,557
            Genes (coding)                    :: 5,320
            CDSs (with protein)               :: 5,320
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 237
            CDSs (without protein)            :: 237
            Pseudo Genes (ambiguous residues) :: 0 of 237
            Pseudo Genes (frameshifted)       :: 102 of 237
            Pseudo Genes (incomplete)         :: 130 of 237
            Pseudo Genes (internal stop)      :: 52 of 237
            Pseudo Genes (multiple problems)  :: 41 of 237
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3136
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267800-rid25106413.denovo.359"
                     /serotype="O 26:H11"
                     /isolate="2201S31241"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-01"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            <1..403
                     /locus_tag="ACHOSC_RS23045"
                     /old_locus_tag="pgaptmp_004609"
     CDS             <1..403
                     /locus_tag="ACHOSC_RS23045"
                     /old_locus_tag="pgaptmp_004609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HGU4550395.1"
                     /translation="SRAVIGWSMSPRMTAQLACDALQMALWWRKRPRNVIVHTDRGGQ
                     YCSADYQAQLKRHNLRGSMSAKGCCYDNACVESFFHSLKVECIHGEHFISREIMRATV
                     FNYIECDYNRWRRHSLCGGLSPEQFENKNLA"
     gene            433..3130
                     /locus_tag="ACHOSC_RS23050"
                     /old_locus_tag="pgaptmp_004610"
                     /pseudo
     CDS             433..3130
                     /locus_tag="ACHOSC_RS23050"
                     /old_locus_tag="pgaptmp_004610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000556531.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter outer membrane beta-barrel
                     domain-containing protein"
BASE COUNT          826 a          692 c          763 g          855 t
ORIGIN      
        1 gtcacgtgcc gttattggct ggtcaatgtc gccacgcatg acggcgcaac tggcctgcga
       61 tgccctgcag atggcgctgt ggtggcgtaa gaggccccgg aacgttatcg ttcacacgga
      121 ccgtggaggc cagtactgtt cagcagatta tcaggcgcaa ctgaagcggc ataatctgcg
      181 tggaagtatg agcgcaaaag gttgctgcta cgataatgcc tgcgtggaaa gcttctttca
      241 ttcgctgaaa gtggaatgta tccatggaga acactttatc agccgggaaa taatgcgggc
      301 aacggtattt aattatatcg aatgtgatta caatcggtgg cggcggcaca gtttgtgtgg
      361 cggcctcagt ccggaacaat ttgaaaacaa gaacctcgct taggcctgtg tccatattac
      421 gtgggtagga tcaactttac tgatggaaca acaaaccagt tgaacatcag caactccctg
      481 attcatggtt caatcacttc aatgctgcct atgggtgaaa gcagtgatgg tcattatgtc
      541 tatcagttct ctgaatacgg tactgatcgt gtaattgatg ataattggca tgatggtgat
      601 gtattcactt taaatattgc taattctaca attgatgacg attatgaagg tttatacttc
      661 actgattcat atctggacgg tgacgttacc aaatatacta acgaaacctt ccatacccca
      721 gctggtgaag gtgaagaata cgccggtttg tttgcaaacg gtggtgttgg ccttgggctg
      781 gcagtgaatc ttgatgtcga aagtaacatc aatatttcta acaactcccg cgttgctggt
      841 atcagcctga ctcagggtaa taccgtaaac aatacttaca ctactgaatc tcatacttgg
      901 gataacaata tctcagttac cgattccaca gtgacttcag gttctgtaac tactctggaa
      961 gacagtggtt tctatggtaa ctctgcggaa cctagtgatt actctggtaa cggcgaagct
     1021 aatgatgtag ctctgtattt cagtgatagt gcggcttcaa attactccat gaaaaacaat
     1081 gtttatttca gcaattctac tctgctgggt gatgttgttt tcgcaagtac tttcaacgca
     1141 aacttctatc ctcaaggtca tgacagtaat gccgatggcg tacttggaca ccaatggtgg
     1201 ctgggctgat gactcactta atgttgatga actgaatatc actctggata atggttctaa
     1261 gtgggtaggc tcagctacta catcagcaaa tgttgatgat gaatctactg cttcgactga
     1321 ctggtatgat gttacaggta atagcctgta tccgggcgtt gtaacagaag caaatgcttg
     1381 gggtcgtacg attgatgatc aagtattcca gagcggtgtg tttaacgtca ctctgaataa
     1441 tggttctgaa tggaataccg ttaacgcttc aaacattgat actttagctg tgaataatgg
     1501 ctctcaggtt aatgtgtctg aatctagctt gatttctgat agcatcgctc tgaccaatgg
     1561 ttcttctttg aacattggag atgaaggtta tgttggaact gatcatctga cagttgatag
     1621 ctacagtact gtagcgttgt ctagtgatga caattcaagt atctataatg gttattacac
     1681 tccgtcgctg ttagcaaata caataactgt gactaacggt ggtttactgg atattaatgt
     1741 agaccagttc gatgataatc cgttccagac tgatactttg gaactgacca gcggcaacat
     1801 cgctgaccat aacggcaacg tagtgtctgg tgtattcgat atccacagca gcgattatgt
     1861 tctgaacgct gatttggtga acgaccgtac ctgggatact tccaagtcta actacggcta
     1921 cggtattgtt gctatgaact ctgatggtca tctgactatc aacggtaacg gtgacatgaa
     1981 caacggcgac gaactggata acagctctgt tgacaacgtt gttgctgcaa ccggtaacta
     2041 caaagttcgt atcgacaacg caacaggcgc tggcgctatc gctgattaca aagataaaga
     2101 aattatctac gtaaacgacg tcaacaccaa cgcgaccttc tctgctgcta acaaagctga
     2161 cctgggtgca tacacctatc aggctgaaca gcgcggtaac accgttgttc tgcaacagat
     2221 ggagctgacc gactacgcta acatggcgct gagcatcccg tctgcgaaca ccaatatctg
     2281 gaacctggaa caagacaccg ttggtactcg tctgaccaac tctcgtcatg gcctggctga
     2341 taacggcggc gcatgggtaa gctacttcgg tggtaacttc aacggcgaca acggcaccat
     2401 caactatgat caggatgtta acggcatcat ggtcggtgtt gataccaaaa ttgacggtaa
     2461 caacgctaag tggatcgtcg gtgcggctgc aggcttcgct aaaggtgaca tgaatgaccg
     2521 ttctggtcag gtggatcaag acagccagac tgcctacatc tactcttctg ctcacttcgc
     2581 gaacaacgtc tttgttgatg gtagcttgag ctactctcac ttcaacaacg acctgtctgc
     2641 aaccatgagc aacggtactt acgttgacgg tagcaccaac tccgacgctt ggggcttcgg
     2701 tttgaaagcc ggttacgact tcaaactggg tgatgctggt tacgtgactc cttacggcag
     2761 cgtttctggt ctgttccagt ctggtgatga ctaccagctg agcaacgaca tgaaagttga
     2821 cggtcagtct tacgacagca tgcgttatga actgggtgta gatgcaggtt ataccttcac
     2881 ctacagcgaa gaccaggctc tgactccgta cttcaaactg gcttacgtct acgacgactc
     2941 taacaacgat aacgatgtga acggtgattc catcgataac ggtactgaag ggtctgcggt
     3001 acgtgttggt ctgggtactc agttcagctt caccaagaac ttcagcgcct ataccgatgc
     3061 taactacctc ggtggtggtg acgtagatca agactggtcc gcgaacgtgg gtgttaaata
     3121 tacctggtaa tattct
//
LOCUS       DAZKHF020000358         3110 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2201S31241
            SAMN43267800-rid25106413.denovo.360, whole genome shotgun sequence.
ACCESSION   DAZKHF020000358 DAZKHF020000000
VERSION     DAZKHF020000358.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267800
            Sequence Read Archive: SRR30307263
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3110)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3110)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 3110)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350581.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350581.2
            Genome Coverage       :: 151x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:21:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,652
            CDSs (total)                      :: 5,557
            Genes (coding)                    :: 5,320
            CDSs (with protein)               :: 5,320
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 237
            CDSs (without protein)            :: 237
            Pseudo Genes (ambiguous residues) :: 0 of 237
            Pseudo Genes (frameshifted)       :: 102 of 237
            Pseudo Genes (incomplete)         :: 130 of 237
            Pseudo Genes (internal stop)      :: 52 of 237
            Pseudo Genes (multiple problems)  :: 41 of 237
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3110
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267800-rid25106413.denovo.360"
                     /serotype="O 26:H11"
                     /isolate="2201S31241"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-01"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            <1..1164
                     /gene="iha"
                     /locus_tag="ACHOSC_RS23055"
                     /old_locus_tag="pgaptmp_004611"
     CDS             <1..1164
                     /gene="iha"
                     /locus_tag="ACHOSC_RS23055"
                     /old_locus_tag="pgaptmp_004611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional siderophore receptor/adhesin Iha"
                     /protein_id="HGU4550396.1"
                     /translation="ENKGRELVRNVLKRDKWGLAGQLRELKESNLILNSLLLTPLGES
                     HLVTVGGEYQSSSMKDGVVLASTGETFRQKSWSVFAEDEWHLTDALALTAGSRYEHHE
                     QFGGHFSPRAYLVWDVADAWTLKGGVTTGYKAPRMGQLHKGISGVSGQGKTNLLGNPD
                     LKPEESVSYEAGVYYDNPAGLNANITGFMTDFSNKIVSYSINDNTNSYINSGKARLHG
                     VEFAGTLPLWSEDVTLSLNYTWTRSEQRDGDNKGAPLSYTPEHMVNAKLNWQITEEVA
                     SWLGARYRGKTPRFTQNYSSLSAVQKKVYDEKGEYLKAWTVVDAGLSWKVTDALTLNA
                     AVNNLLNKDYSDVSLYSAGKSTLYAGDYFQTGSSTTGYVIPERNYWMSLNYQF"
     gene            complement(1418..1775)
                     /locus_tag="ACHOSC_RS23060"
                     /old_locus_tag="pgaptmp_004612"
                     /pseudo
     CDS             complement(1418..1775)
                     /locus_tag="ACHOSC_RS23060"
                     /old_locus_tag="pgaptmp_004612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001368704.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
     gene            1995..2759
                     /locus_tag="ACHOSC_RS23065"
                     /old_locus_tag="pgaptmp_004613"
                     /pseudo
     CDS             1995..2759
                     /locus_tag="ACHOSC_RS23065"
                     /old_locus_tag="pgaptmp_004613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001297906.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS110 family transposase"
BASE COUNT          791 a          783 c          794 g          742 t
ORIGIN      
        1 gaaaataaag gtcgtgagct tgtacgcaat gtactgaaac gcgacaaatg gggccttgcc
       61 ggtcaactgc gggagcttaa ggaatctaac cttatcctga attcattgct gcttacccct
      121 ctgggagaat ctcatctggt tactgtgggg ggcgagtatc agagttcgtc catgaaagat
      181 ggggttgtcc ttgccagcac aggtgaaact ttccggcaga aaagctggtc ggtatttgct
      241 gaggacgagt ggcatctcac ggatgcactt gcgctgactg cgggcagccg ctatgaacat
      301 catgagcaat tcggggggca cttcagtccg cgtgcatatc tggtctggga tgtggcagat
      361 gcctggacgc tgaaaggcgg tgtgaccacg ggatataagg cacccagaat ggggcagctc
      421 cataaaggga ttagtggtgt gtccgggcag ggaaaaacaa atctacttgg taaccccgac
      481 ctgaagccgg aagagagcgt cagttatgag gctggggtgt attacgataa ccccgccggt
      541 ctgaacgcca atatcacagg ttttatgact gacttctcta acaagattgt ctcttattcc
      601 ataaatgata acaccaatag ctatataaac agcggaaagg cccggttgca cggtgtggaa
      661 tttgccggca cattgccgct atggtcagag gatgtcacgc tgtcgctgaa ttacacctgg
      721 acccgaagtg aacaacgtga tggtgataat aaaggtgcac cgctgagtta taccccagaa
      781 cacatggtga atgcgaaact gaactggcag atcaccgaag aggtggcatc atggctgggt
      841 gcccgttatc gcgggaaaac accacgtttc acccagaatt attcgtcgct gagcgctgta
      901 cagaagaaag tatatgatga gaaaggagaa tacctgaaag cctggacggt ggtggatgca
      961 ggtctgtcgt ggaaggtgac ggatgccctg acgctgaatg cggcagtgaa taacctgctc
     1021 aacaaggatt acagtgacgt gagtctgtac agtgccggta agagtacgct ttatgccggt
     1081 gattacttcc agacgggatc atcaacaacg ggatatgtga tacctgagcg aaattactgg
     1141 atgtcgctga actatcagtt ctgataacaa caaaacgcta tcactgacgg tagcatacgt
     1201 tgccactgca actcctggcg gaacggtggc aatgttttag gttaagtgcg tttccggtcc
     1261 gctaatgaga tttcgttacc aacaactaat atcatcaccg gaaatgcacg gattattttt
     1321 taactcatca tttacatact tgtccagaaa tttatactcg gctagctctg aattaagggc
     1381 cgcgtccgca tctccattac cgtcttatat cactggcgct gacaattgtc tcacatatag
     1441 ctgcagtatc ggctccatcc ccgtggctac gcgctgaaat tctagtgaac cgctttcgtg
     1501 gacttgagtg aaccaactgt ttagctgtga tttatttggg gggcagaata cggtagaact
     1561 cgagtgccag ttgctgcgct attttctgtt ccggttctgt ttcacaaatc gggacgcata
     1621 attttcttcc ccctgattat tttccagggc accaccccgc agcttcccag ggacagtgaa
     1681 ggaagtcggg cggcaatagc aaacggcggg atccaacctt tacgccattt ggcaacagta
     1741 tctctgatga tagtttcagt gcctgtaact ccccgttact ttattcgtac ccttgtaatg
     1801 gggtgttagc cagccccggc atgattaatt cccccagtcg tccatgattc ggggggatgt
     1861 caccgggtct ggtggggcgc tggtaaccgc tgataggggc ctggtcagac atcatgtcgg
     1921 gacagtctgt aacgtggatg ccggtacctg ctccccaagg ttatctggtt aacccgtata
     1981 aacggggaga cacaatgccc gaatccagcg attacgaatc aatcctggtc tttattggcg
     2041 ttgatgtagg taaagacacc cattacgccg ttgccgttaa tcgttcaggt aaacgtttgt
     2101 tcgacagagt attacccaac gacgaaaaca aactcagagc gctgatatct gacctgaaac
     2161 agcatggaca gatactgctg gtcgttgacc agcctgctac catcagggcg ttacccgtcg
     2221 ccgttgcccg ctctgaaggc gtccttgtcg gataccttcc tggactggca atgcgacgca
     2281 tcgccgattt gcactccggt gaagccaaaa ccgatgcccg tgatgcggcc atcattgccg
     2341 aagctgcacg tactctgcct catgctcttc gctctctaaa actggccgac gaacaaatcg
     2401 ccgaactctc catgctttgc ggcttcgatg atgatcttgc cgcacagaca acacaggcca
     2461 gcaaccgcat ccgcggcttg ttgacccaga tacatccggc tctggagcgc gttctcggcc
     2521 cgcgactgga tcatcctgca gtacttgatc ttctccagcg atatccctca cccgaaaaac
     2581 tcgctttact gggtgagaaa aaactggcaa cccagctctg caaactcgcg ccccgtctgg
     2641 gaaaacgcct tgctgcagat atcccccagg cactgacaca gcaaaccgtc atcgtccccg
     2701 gtacgaatgc tgctgtcgtt gttctcccac gcctggcact ccagctcatc acgctgcgtt
     2761 agccaagaga cgaagtgact ctggcttcaa ctataagttt gttaatacca gtgacgctga
     2821 tgtcgttaca ggcgatagca caggacgcaa gacgatggta acggtaatag agaggcagat
     2881 gaaccatcca ctggaagatt gcggacctct cttaaaatta gtacattacg gaccgccacg
     2941 ctggaaagac agcatatgga aaggctccct gtcaaacatt ttgtttaacc cggaccctga
     3001 ccatctaacc ttggcaagtg gaagattagc gcctctttga aaaaggatca aacacaacac
     3061 agcagaaaac gattatagtg aatattgaaa ctatgtgctt atcaaaaaat
//
LOCUS       DAZKHF020000359         3092 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2201S31241
            SAMN43267800-rid25106413.denovo.361, whole genome shotgun sequence.
ACCESSION   DAZKHF020000359 DAZKHF020000000
VERSION     DAZKHF020000359.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267800
            Sequence Read Archive: SRR30307263
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3092)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3092)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 3092)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350581.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350581.2
            Genome Coverage       :: 151x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:21:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,652
            CDSs (total)                      :: 5,557
            Genes (coding)                    :: 5,320
            CDSs (with protein)               :: 5,320
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 237
            CDSs (without protein)            :: 237
            Pseudo Genes (ambiguous residues) :: 0 of 237
            Pseudo Genes (frameshifted)       :: 102 of 237
            Pseudo Genes (incomplete)         :: 130 of 237
            Pseudo Genes (internal stop)      :: 52 of 237
            Pseudo Genes (multiple problems)  :: 41 of 237
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3092
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267800-rid25106413.denovo.361"
                     /serotype="O 26:H11"
                     /isolate="2201S31241"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-01"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            3..1902
                     /gene="espL1"
                     /locus_tag="ACHOSC_RS23070"
                     /old_locus_tag="pgaptmp_004614"
                     /pseudo
     CDS             3..1902
                     /gene="espL1"
                     /locus_tag="ACHOSC_RS23070"
                     /old_locus_tag="pgaptmp_004614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310454.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system effector EspL1"
     gene            2074..2181
                     /gene="yniD"
                     /locus_tag="ACHOSC_RS23075"
                     /old_locus_tag="pgaptmp_004615"
     CDS             2074..2181
                     /gene="yniD"
                     /locus_tag="ACHOSC_RS23075"
                     /old_locus_tag="pgaptmp_004615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588455.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="small membrane protein YniD"
                     /protein_id="HGU4550397.1"
                     /translation="MPTKRFDKKHWKMVVVLLAICGAMLLLRWAAMIWG"
     gene            complement(2234..2992)
                     /locus_tag="ACHOSC_RS23080"
                     /old_locus_tag="pgaptmp_004616"
     CDS             complement(2234..2992)
                     /locus_tag="ACHOSC_RS23080"
                     /old_locus_tag="pgaptmp_004616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdiY family protein"
                     /protein_id="HGU4550398.1"
                     /translation="MKLLKTVPAIVMLAGGMFASLNAAADDSVFTVMDDPASAKKPFE
                     GNLNAGYLAQSGNTKSSSLTADTTMTWYGQTTAWSLWGNASNTSSNDERSSEKYAAGG
                     RSRFNLTDYDYLFGQASWLTDRYNGYRERDVLTAGYGHQFLNGPVHSFRFEFGPGVRY
                     DKYTDNASETQPLGYASGAYAWQLTDNAKFTQGVSVFGAEDTTLNSESALNVAINEHF
                     GLKVAYNVTWNSEPPESAPEHTDRRTTLSLGYSM"
BASE COUNT          934 a          701 c          608 g          849 t
ORIGIN      
        1 ttatgagtca aaacgatatc attatcagaa ctcattataa gtctcctcat agattgcaca
       61 tcgatagcga cataccaaca ccttcatcag agcctattaa taaatttgcg cgccagctca
      121 tcaccctact tgatacctct gacttaagtt cgatgctgtc atactgtgtt actcaggaat
      181 ttaccgcaaa ctgtcgaaaa atatcacaaa attgttattc cactgccctt tttaccatta
      241 accttgccac ttcacccatc catgcagaaa atatactcat tacattacac tataaaaaag
      301 atatcatttc cttattactg gaaaccacgc ctattaaagc taaccatttg cgaagcatac
      361 tggattatat tgaacaggaa cagttaactg ctgaagatcg taaccattgt atgaaactgt
      421 ctaaaaaaat ccatagagaa aaaactatac acccaacagt aaatctcaat ggtagtgcat
      481 gttttttgca atctccttct gacgctattt tttgtcgcca tctgtcattg caatacgccc
      541 ttgattcatt gagaaatgga aaaggcaaag tcaacctgat taaacattac tcctccgttg
      601 aatccataca gcagcatgtc cccttagtcc gggacgcgga gttcagagca ttacttcgcc
      661 atcctcctgc agggagtcgc gttatcgcga gtaaggattt tggcttcgct ttagattttt
      721 tcttttgtcg aatgatggca aacaatatca gtcatatgtc cgcgatttta tatatagaca
      781 atcatacttt gtcagtaagg ctacgaataa agcagtcagt gtatggacaa ttaaactatg
      841 ttgtgtccgt ttacgacccg aacgatacca acgttgccgt cagagacacc cacaggacag
      901 cacggggctt tctttcgctt gataagttca tcagttcagg tcccgatgct cagacctggg
      961 ctgataggta tgttcgcaac tgtgcaattg ctattctgcc cctattacct gtgggagttc
     1021 caggggctat tttcgcgggt attgcatcac gaatgccatt tgcccctata catccatcgg
     1081 caatgttgtt aataatggct acaggccaga ctcaacagct tattacatta ttcaaacagt
     1141 tgcccatact ccctgaaaaa gaaatcattg aaataataac tgcgcagaat agcgttggta
     1201 cacctgcttt atttttggct atgatgaacg gacatactga caacgtgaaa atatttatgc
     1261 aagaaattca gtcactggta gataatcaca tcattcatga agataacctg gttaaattac
     1321 tgcaaactaa aagtgctaac gaaacacctg gactctatat ctccatgttg tatggattcg
     1381 atgaaataat cgatatcttt ctgaatgcat taaccactcc aatagcacaa gaacttttaa
     1441 acaaaaaaat ggtgatggat attttagcca tgaaaacacg tgatggtgaa ccagggttat
     1501 ttgctgcaat ggaaaataat caccctttgt gtgtcacacg gttcctctct aaagtttatg
     1561 ggatcgccgt taaatataaa ctcagcaaaa tcaacatcat ggacttatta aaaggcgcga
     1621 cagcacaggg aacccctgca ttatacatcg ccatgagcaa gggtaatgaa gacgtcgtgt
     1681 tatcttatat atcgacgctg ggtgcttttg caaaaaaaac attcttttag tcaacatcag
     1741 ttatttacac tattggccgc taaaaatcat gacaacatgt cagctgttca tatagccatt
     1801 catcataagc attataaaac tgtagaaaca tattatgctg ctataaatgt catcagccaa
     1861 agcctgagtt ttagtgctga tgaaataaag acgtatttat aaccagctat ttacgatata
     1921 tatcgataca taattttatt tttttcataa aacgcagaaa cagtgaccag gtgtacaacg
     1981 ccacccaacg gctgtaataa caaaaaatcc cttgatgccc gtcccttttg ttacactccg
     2041 ttatcacgca caagagatat gcaggacact ggtatgccga ctaaacgctt tgataaaaaa
     2101 cactggaaga tggtggtggt gctactggca atctgtggcg ctatgttgtt gctacgttgg
     2161 gcagcaatga tttggggctg agcagtgaca aacgaagaca gccagaccgc ataacacggt
     2221 ccggcagata attttacatg ctgtagccca gcgataaggt tgtccgacgg tcggtatgtt
     2281 ccggtgcaga ctctggaggt tcagagttcc atgtgacgtt gtaagccacc ttcagtccaa
     2341 aatgttcatt aatagcaaca tttaatgcgc tttcggagtt cagcgttgtg tcttccgcgc
     2401 caaagaccga aacaccctgc gtaaatttag cgttgtcagt caactgccat gcataagccc
     2461 cggaggcata gcccagcggc tgggtttcac tggcattgtc ggtatatttg tcgtaacgca
     2521 cacctggacc gaattcaaag cggaaactgt gtaccgggcc attcaaaaac tgatgaccat
     2581 aacccgcggt caacacatcg cgctctcgat agccgttata acggtctgtc agccagcttg
     2641 cctgaccaaa taaatagtca taatcagtta aattaaaacg gctacgcccg cccgccgcat
     2701 atttttctga agaacgctca tcattagaag aagtattact ggcgttcccc cacagcgacc
     2761 aggcagtggt ttgtccatac caggtcatgg tggtgtcagc cgtaagtgag gagcttttcg
     2821 tattgcctga ttgtgcaaga tatcctgcgt tcagattacc ttcgaaaggt tttttagcgc
     2881 tggcaggatc gtccatgaca gtaaaaacgg aatcatcggc agctgcattc agtgacgcaa
     2941 acatgccccc cgccaacata acgatggcag gaactgtctt caaaagcttc atttatcaag
     3001 agtccgtaca acaaaaaaag agaccatcgc ggtcccggaa actttcttaa ggatcaaaga
     3061 ttagcgtccc tggaaaggta acgaattata aa
//
LOCUS       DAZKHF020000360         3076 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2201S31241
            SAMN43267800-rid25106413.denovo.362, whole genome shotgun sequence.
ACCESSION   DAZKHF020000360 DAZKHF020000000
VERSION     DAZKHF020000360.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267800
            Sequence Read Archive: SRR30307263
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3076)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3076)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 3076)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350581.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350581.2
            Genome Coverage       :: 151x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:21:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,652
            CDSs (total)                      :: 5,557
            Genes (coding)                    :: 5,320
            CDSs (with protein)               :: 5,320
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 237
            CDSs (without protein)            :: 237
            Pseudo Genes (ambiguous residues) :: 0 of 237
            Pseudo Genes (frameshifted)       :: 102 of 237
            Pseudo Genes (incomplete)         :: 130 of 237
            Pseudo Genes (internal stop)      :: 52 of 237
            Pseudo Genes (multiple problems)  :: 41 of 237
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3076
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267800-rid25106413.denovo.362"
                     /serotype="O 26:H11"
                     /isolate="2201S31241"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-01"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            <1..261
                     /locus_tag="ACHOSC_RS23085"
                     /old_locus_tag="pgaptmp_004617"
     CDS             <1..261
                     /locus_tag="ACHOSC_RS23085"
                     /old_locus_tag="pgaptmp_004617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="host specificity protein J"
                     /protein_id="HGU4550399.1"
                     /translation="GSGNNPGGTRYTGYGFEVRKNGVLIASRETKGAIPGSYSAVIDM
                     PSGGGSVTLEFKIFQKGNQGAGNITDCTVIVTKKAASGISIR"
     gene            332..931
                     /locus_tag="ACHOSC_RS23090"
                     /old_locus_tag="pgaptmp_004618"
     CDS             332..931
                     /locus_tag="ACHOSC_RS23090"
                     /old_locus_tag="pgaptmp_004618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044384 - host outer membrane [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Ail/Lom family outer membrane beta-barrel
                     protein"
                     /protein_id="HGU4550400.1"
                     /translation="MRKVCAAILSAAICLAVSGAPAWASEQQATLSAGYLHARTNAPG
                     SDNLNGINVKYRYEFTDTLGLITSFSYANAEDEQKTHYSDTRWHEDSVRNRWFSVMAG
                     PSVRVNEWFSAYAMAGMAYSRVSTFSGDYIRVTDNKGKTHDVLTGSDDGRHSNTSLAW
                     GAGVQFNPTESVAVDVAYEGSGSGDWRTNSFIVGVGYKF"
     gene            996..>3076
                     /locus_tag="ACHOSC_RS23095"
                     /old_locus_tag="pgaptmp_004619"
     CDS             996..>3076
                     /locus_tag="ACHOSC_RS23095"
                     /old_locus_tag="pgaptmp_004619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prophage tail fiber N-terminal domain-containing
                     protein"
                     /protein_id="HGU4550401.1"
                     /translation="MAVKISGVLKDGTGKPVQNCTIQLKAKRNSTTVVVNTLASENPD
                     EAGRYSMDVEYGQYSVILLVEGFPPSHAGTITVYEDSRPGTLNDFLGAMTEDDARPEA
                     LRRFELMVEEVARNASAVAQNTAAAKKSAGDAGTSAREAATHATDAAGSARAASTSAG
                     QAATSAQSASSSAGTASTKATEASKSAAAAESSKSAAATSAGAAKTSETNAAASQKSA
                     ATSASTATTKASEAATSARDAAASKEAAKSSETNASTSASSAASSAMAAGNSVKAAKT
                     SETNAKSSETAAEQSASAAADSETAAASSAREASIKAEEAAVSATAAGKSAESAASSA
                     STATTKAGEATEQASAAARSASAAKTSETNAKASETSAESSKTAAASSASSAASSASS
                     ASASKDEATRQASAARGSATTASTKATEAAGSAAAAAQSKSTAESAATRAETAAKRAE
                     DIASAVALEDASTTKKGVVQLSSATNSTSESQAATPKAVKAAYDLANGKYTAQDATTG
                     QKGIVQLSSATNSTSESQAATPKAVKAAYDLANGKYTAQDATTAQKGIVQLSSATNST
                     SETLAATPKAVKAANDNANGRVPSARKVNGKALSADITLTPKDIGTLNSTTMSFSGGA
                     GWFKLATVTMPQASSVVSITLIGGAGFNVGSPQQAGISELVLRAGNGNPKGITGALWQ
                     RTLTGFTNFAWV"
BASE COUNT          811 a          790 c          906 g          569 t
ORIGIN      
        1 gggagcggaa ataacccagg aggcacgcga tacacgggtt atggttttga agttcgcaaa
       61 aacggcgtat taatcgcatc cagagaaact aaaggggcca ttcccggtag ttacagtgca
      121 gttattgata tgcctagtgg tggtggtagc gtcactctgg agtttaagat tttccagaaa
      181 ggcaatcagg gggcaggcaa tatcaccgat tgtacggtga ttgtgaccaa aaaagctgct
      241 tccggcatca gtattcgttg aaatatttat aaccccaata aagggcgtca ggaatgacgc
      301 cttttttatt gcagaaaagc gagaggtaat tatgcgtaaa gtttgtgcag ccattttgtc
      361 cgcagccatc tgtctggccg tatccggtgc gcctgcatgg gcgtctgagc agcaggccac
      421 actgagcgca gggtatcttc atgcccgtac gaacgctccc ggcagcgata atctgaacgg
      481 gattaacgtg aaataccgtt atgagtttac ggacacgctg gggctgatta cgtcattcag
      541 ttatgccaac gctgaagatg agcaaaaaac gcattacagc gatacccgct ggcatgaaga
      601 ttccgtgcgt aaccgctggt tcagcgtgat ggcggggccg tctgtgcgcg tgaatgaatg
      661 gttcagcgcg tatgcgatgg cgggtatggc ttacagccgt gtttcgactt tctccggaga
      721 ttatatccgc gtaactgaca acaaggggaa aacgcacgat gtgctgaccg gaagtgatga
      781 cggtcgccac agcaacacgt ctctggcgtg gggagctggc gtgcagttta acccgaccga
      841 atccgtggcc gttgacgtcg cttatgaagg ctccggcagc ggtgactggc gtaccaacag
      901 tttcatcgtg ggtgtcggtt ataaattctg attagccagg taacacagtg ttatgacagc
      961 ccgccggttc aggcgggctt ttttgtgggg tgaatatggc agtaaagatt tcaggtgtac
     1021 tgaaagacgg cacaggaaaa ccggtacaga actgcacaat ccagctgaaa gcaaaacgta
     1081 acagcaccac ggtggtggtg aacacgctgg cctcagaaaa tccggatgaa gccgggcgtt
     1141 acagtatgga cgttgagtac ggtcagtaca gcgttattct gttggtggaa ggattcccgc
     1201 cgtcacatgc cgggaccatc accgtgtatg aagattcccg acccggtacg ctgaatgatt
     1261 ttctcggtgc catgacggag gatgatgccc gtccggaggc actgcgccgt tttgagctga
     1321 tggtggaaga ggtggcgcgt aacgcgtccg cggtggcaca gaacacggca gccgcgaaga
     1381 agtcagccgg cgatgccggc acatcagccc gtgaggcggc aacccatgcg actgatgctg
     1441 caggctcagc acgtgcagcc agcacatcag ccgggcaggc cgcgacgtcg gctcagtcag
     1501 cgtcttccag cgcaggaaca gcatcaacaa aggccacaga agcatcaaaa agtgctgccg
     1561 ctgcagagtc ctcaaaaagc gcggcggcca ccagtgccgg tgccgcgaaa acgtcagaaa
     1621 cgaatgctgc agcgtcacaa aaatcagcag ccacttctgc atccactgcg acgacgaaag
     1681 cgtcagaagc cgccacttca gccagagatg cggcggcctc caaagaggcg gcaaaatcat
     1741 cagaaacgaa cgcctccaca agcgccagca gtgcagcctc ctcggcaatg gcggcaggaa
     1801 attccgtgaa ggcggcaaaa acgtccgaga cgaatgctaa gtcttctgaa acggcagcgg
     1861 aacagagcgc ctcagctgcg gcagactctg agacagcagc tgcatcatcg gcccgtgagg
     1921 cgtccattaa ggcagaggag gcagcagtca gtgccaccgc cgccggaaaa tcggcagaaa
     1981 gcgccgcatc atccgcttca acagccacaa cgaaggctgg cgaagccact gaacaggcca
     2041 gcgcagcagc gaggtctgct tccgcagcga agacatccga aacgaacgcg aaagcgtcgg
     2101 aaaccagcgc agaatcctca aaaacggctg ccgcatcgtc cgccagttcg gcggcgtcat
     2161 cggcatcatc tgcgtctgct tcaaaagatg aggcgaccag acaggcgtca gcagcgaggg
     2221 gcagcgccac gacggcatcc acgaaggcga cagaggctgc tggcagtgcg gcagcggcag
     2281 cacagagcaa aagtacggca gaatccgcag caacgcgcgc tgagacagcg gcaaaacgtg
     2341 cagaggatat tgcatccgcc gtggcgcttg aggatgcgag cacgacgaaa aagggggtag
     2401 tacagctcag cagtgcgacc aacagcactt ccgagtcaca ggcggcaacg ccaaaagccg
     2461 ttaaggccgc gtatgacctg gctaacggga aatacaccgc acaggatgca acgacaggac
     2521 agaaagggat agttcagctc agcagtgcga ctaacagcac ttccgagtca caggcggcaa
     2581 cgccaaaagc cgttaaggcc gcgtatgacc tggctaacgg gaaatacacc gcacaggacg
     2641 ctacgacagc acaaaaagga attgtccagc tcagtagtgc aaccaacagc acatctgaaa
     2701 cgcttgctgc gacaccgaaa gcagtgaaag cagctaatga caatgcgaat ggtcgggtac
     2761 cttctgcccg taaggtgaat ggtaaggcgc tttcagcgga tataacactg acgccgaaag
     2821 atattggtac gcttaactca acaacaatgt cattcagcgg tggtgctggt tggttcaaat
     2881 tagcaacggt aaccatgcca caggcgagtt ctgttgtttc aattacgttg attggtggcg
     2941 ctggatttaa cgtggggtca cctcaacagg caggtatatc tgaacttgtt ttacgtgcag
     3001 gtaatggtaa tccgaagggg attactggtg ctttatggca gcgcacattg acagggttta
     3061 caaattttgc ctgggt
//
LOCUS       DAZKHF020000361         3074 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2201S31241
            SAMN43267800-rid25106413.denovo.363, whole genome shotgun sequence.
ACCESSION   DAZKHF020000361 DAZKHF020000000
VERSION     DAZKHF020000361.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267800
            Sequence Read Archive: SRR30307263
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3074)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3074)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 3074)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350581.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350581.2
            Genome Coverage       :: 151x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:21:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,652
            CDSs (total)                      :: 5,557
            Genes (coding)                    :: 5,320
            CDSs (with protein)               :: 5,320
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 237
            CDSs (without protein)            :: 237
            Pseudo Genes (ambiguous residues) :: 0 of 237
            Pseudo Genes (frameshifted)       :: 102 of 237
            Pseudo Genes (incomplete)         :: 130 of 237
            Pseudo Genes (internal stop)      :: 52 of 237
            Pseudo Genes (multiple problems)  :: 41 of 237
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3074
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267800-rid25106413.denovo.363"
                     /serotype="O 26:H11"
                     /isolate="2201S31241"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-01"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            complement(1..521)
                     /locus_tag="ACHOSC_RS23100"
                     /old_locus_tag="pgaptmp_004620"
                     /pseudo
     CDS             complement(1..521)
                     /locus_tag="ACHOSC_RS23100"
                     /old_locus_tag="pgaptmp_004620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001178393.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing N-terminus and C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS481 family transposase"
     gene            complement(755..1432)
                     /locus_tag="ACHOSC_RS23105"
                     /old_locus_tag="pgaptmp_004621"
     CDS             complement(755..1432)
                     /locus_tag="ACHOSC_RS23105"
                     /old_locus_tag="pgaptmp_004621"
                     /inference="COORDINATES: protein motif:HMM:NF033590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS4 family transposase"
                     /protein_id="HGU4550402.1"
                     /translation="MWHYLHYKVSRGKPHAECDAKRRAGSPPGPDAHQLQRSQHKKSR
                     QQRPGALAHVCLLRGAGEDGACWYPLTSEKVASAADARRIVSHYEQRWLIEEYHKAWK
                     SGGTCVESLRMQTRDNLERMVVIAVRVLGLRQGSISEETQNDSCEKILTPTEWKLLWV
                     KLEGKQLPAQTPTLKWACLKLAKLGRWHDSKRTSSPGWVVMWDGWFRHQDMAEGYLVM
                     KSLDQEI"
     gene            1630..>3074
                     /locus_tag="ACHOSC_RS23110"
                     /old_locus_tag="pgaptmp_004622"
     CDS             1630..>3074
                     /locus_tag="ACHOSC_RS23110"
                     /old_locus_tag="pgaptmp_004622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001368704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3-like element ISEc38 family transposase"
                     /protein_id="HGU4550403.1"
                     /translation="MGNSMHSLKTLLQLPCGWRCSRQIISSDGITLHLHGKRKKAQCP
                     ECFKRSDSVHSCRRRRIQHLPCSGHTLWLVFSVRHWYCRNPACSRKIFAGSLAPFAGS
                     HQQSSQALQNLQRQLGLIAGGEAGKRAATAVGLRCSADTLLRRVINTPETKQSGAPHV
                     GIDEWAWHRGHRYGTLIVNLDTHRPLVLLPGRDQRTLATWFRKYPEIQIVSRDRSGVY
                     ATAAREGAPQARQVADRWHLLKNIGDALERMMYRHIPLIRLVASELSPKKSPDPEPSV
                     PAASLRRPERLKQQTRKKRHQRWTEVMALHNKECSFREISRITGLSRVTVSRWVRSGT
                     FPEMSTRPPKRGLLDPWREWLKEQRESSNYNASRIWREMVARGFTGSETIVRDAVAKW
                     RKGWNPPVTTAVRLPSVSRVSRWLMPWRITRDEENYASRFISLMCEKEPELKIAQQLA
                     LEFYRILKTQNKSQLSSWFNRVHESGSAEFRR"
BASE COUNT          753 a          786 c          726 g          809 t
ORIGIN      
        1 acagtggaaa taatatattc tactgatttt tgtagtttaa acgatttatg aagaatttta
       61 ctgttgtaca gtaccagata ctcagccagt ttcagattga acatattcag ctattcaaag
      121 agcaattctt cattaaactc aataaattgt tccctcagtg ttcggttaaa atgctcactt
      181 atggcattta ttttcggcgt atagagataa gtccatacga acccgacggt ggcttcctgt
      241 agcgttttgt cgaattttcc cgagaactct ttgcccatca tcagtgatga tctggttgat
      301 accaacaagg aacaactggg ctacacagca gaagaaatgc ctgatgatat cacttttaag
      361 cgatagtacg gccagagagt agttgctgtg ttcgtcgagc atggtaatga aataacggta
      421 cagctcgcca ttctgagctc aatagtatcc atgccactga gcttacaggt ctttatcggg
      481 cgatagtgct ttattcttcg gggctttgtg gatgattttt cgaaatgttc atgatagtta
      541 ggtaaagcta ccgcatatca tttgcaaaat gacagataga tatacttcaa tttgaatctt
      601 aacaattagt atgggaatgc tgtcaatcct ctgtatgcgt ccaccacata ttgaaatttt
      661 tgttctctaa tatattccac gggcgtcaag agtgtgaggc ctgtgctgtt atacagcacc
      721 agacgttgtc ccccatcttt gacctgtctc ttgatcagat ctcctgatca agagacttca
      781 tcaccaggta accctcagcc atatcctgat gtctgaacca gccatcccac ataactaccc
      841 aaccggggct gcttgtgcgt ttgctgtcat gccatcgccc cagtttagcc agtttcaaac
      901 aggcccattt cagggtcggt gtttgcgctg gaagctgctt tccttccagc ttaacccaca
      961 gtagtttcca ctctgtcggc gtcagtattt tctcacagct gtcattttgt gtttcttcac
     1021 tgatacttcc ctgccgcagg cccagcaccc gcaccgcgat aaccaccatg cgctcaaggt
     1081 tatcccgggt ctgcattcgc agcgattcca cacatgtacc accacttttc cacgctttgt
     1141 ggtattcctc tatcagccag cgttgctcgt aatggctgac gatacgtcgc gcatcggcgg
     1201 cactcgccac tttttctgat gtcagcggat accagcaggc tccgtcctct cctgctcccc
     1261 gcaacagaca tacgtgagcg agagcgcctg gccgctgttg tcgggatttt ttatgctgac
     1321 ttcgctgtaa ctgatgagca tccgggcctg gcgggctgcc cgcccgcctt tttgcatcac
     1381 attcagcgtg tggcttcccg cggctgactt tataatgcag gtaatgccag atatccgctt
     1441 cccggtcaca gacagtgatt acctgtttct gtatctcccc cagccgttcg gccatgcgct
     1501 ccgaagcctg ctgccagcgg taactttctt tttctgtcac gaacggtgca atagtggttc
     1561 ttccgcaact tgggtggaag aaaatggatg aaactttctg gtgaacaaag atctaagctg
     1621 ttttaagtca tgggcaactc aatgcactct cttaagacac ttctacagtt accttgcgga
     1681 tggcgatgca gtcgacaaat tattagctct gacggtataa ccctccatct ccacggaaaa
     1741 cgcaaaaaag cacaatgtcc tgaatgcttt aagcgtagcg actctgttca tagttgtcgt
     1801 cggcgccgga tacagcatct accctgctca ggacatacgc tatggcttgt attttccgtc
     1861 cgccactggt actgccgtaa cccggcttgt tcacgtaaaa tttttgccgg gtcgcttgct
     1921 cccttcgccg gttcacacca acagtcttca caggcgttac aaaatttaca acgtcaactg
     1981 ggattaatcg ccggaggtga ggccgggaaa cgggcagcaa cggcagtggg gctccgttgc
     2041 agtgctgata ctctccttcg cagggttatc aataccccgg agacgaaaca gtcaggcgcg
     2101 cctcatgtcg gtattgatga gtgggcgtgg catcggggcc accgttacgg tacgttaatc
     2161 gtcaatcttg atactcaccg tcccctcgtc ctgcttcccg gccgtgatca gcgtacgctg
     2221 gcgacctggt tcagaaaata tccggaaata caaattgtct cgcgtgatcg cagtggagtc
     2281 tatgcgacag cagcacgtga aggtgcacct caggccagac aggtggccga tcgatggcat
     2341 ctgctaaaaa atattggcga tgcgcttgaa cgaatgatgt acagacatat acctctgata
     2401 cgtcttgttg ccagtgagtt gtcaccaaag aaatcacctg atccagaacc atctgtgcct
     2461 gcagcatcgc tccgtcgtcc ggaacgccta aaacaacaaa cccgcaaaaa acggcatcag
     2521 cgttggacag aggttatggc cctgcataac aaggaatgta gtttcaggga aatatcccgt
     2581 attacaggcc tgtcgcgtgt gacagtcagt cgctgggtgc gttcaggaac attccctgaa
     2641 atgtcaaccc gacctccaaa gcgagggctt ctggacccat ggagggagtg gttaaaagag
     2701 caacgagaaa gtagtaatta taacgccagc cggatatggc gggaaatggt ggcccgggga
     2761 tttacaggca gtgaaaccat cgtcagggat gcggttgcca aatggcgtaa aggctggaac
     2821 ccaccggtta ctactgccgt cagacttcct tcagtgtccc gggtaagccg ctggttgatg
     2881 ccctggagaa taaccaggga tgaagaaaat tatgcttccc gatttattag tctgatgtgt
     2941 gaaaaagaac ctgagctgaa aatagcgcag caactggcac tcgagttcta ccgtattctg
     3001 aaaacccaaa ataaatcaca gctaagcagt tggttcaatc gagtccacga aagcggttca
     3061 gcagaatttc ggcg
//
LOCUS       DAZKHF020000362         3061 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2201S31241
            SAMN43267800-rid25106413.denovo.364, whole genome shotgun sequence.
ACCESSION   DAZKHF020000362 DAZKHF020000000
VERSION     DAZKHF020000362.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267800
            Sequence Read Archive: SRR30307263
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3061)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3061)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 3061)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350581.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350581.2
            Genome Coverage       :: 151x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:21:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,652
            CDSs (total)                      :: 5,557
            Genes (coding)                    :: 5,320
            CDSs (with protein)               :: 5,320
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 237
            CDSs (without protein)            :: 237
            Pseudo Genes (ambiguous residues) :: 0 of 237
            Pseudo Genes (frameshifted)       :: 102 of 237
            Pseudo Genes (incomplete)         :: 130 of 237
            Pseudo Genes (internal stop)      :: 52 of 237
            Pseudo Genes (multiple problems)  :: 41 of 237
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3061
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267800-rid25106413.denovo.364"
                     /serotype="O 26:H11"
                     /isolate="2201S31241"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-01"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            30..527
                     /gene="ftnA"
                     /locus_tag="ACHOSC_RS23115"
                     /old_locus_tag="pgaptmp_004623"
     CDS             30..527
                     /gene="ftnA"
                     /locus_tag="ACHOSC_RS23115"
                     /old_locus_tag="pgaptmp_004623"
                     /EC_number="1.16.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008199 - ferric iron binding [Evidence
                     IEA];
                     GO_process: GO:0006879 - intracellular iron ion
                     homeostasis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="non-heme ferritin"
                     /protein_id="HGU4550404.1"
                     /translation="MLKPEMIEKLNEQMNLELYSSLLYQQMSAWCSYHTFEGAAAFLR
                     RHAQEEMTHMQRLFDYLTDTGNLPRINTVESPFAEYSSLDELFQETYKHEQLITQKIN
                     ELAHAAMTNQDYPTFNFLQWYVSEQHEEEKLFKSIIDKLSLAGKSGEGLYFIDKELST
                     LDTQN"
     gene            complement(565..804)
                     /locus_tag="ACHOSC_RS23120"
                     /old_locus_tag="pgaptmp_004624"
     CDS             complement(565..804)
                     /locus_tag="ACHOSC_RS23120"
                     /old_locus_tag="pgaptmp_004624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YecH family metal-binding protein"
                     /protein_id="HGU4550405.1"
                     /translation="MDSIHGHEVLNMMIESGEQYTHASLEAAIKARFGEQARFHTCSA
                     EGMTAGELVAFLAAKGKFIPSEEGFSTDQSKICRH"
     gene            995..2206
                     /gene="tyrP"
                     /locus_tag="ACHOSC_RS23125"
                     /old_locus_tag="pgaptmp_004625"
     CDS             995..2206
                     /gene="tyrP"
                     /locus_tag="ACHOSC_RS23125"
                     /old_locus_tag="pgaptmp_004625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015173 - aromatic amino acid
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0015801 - aromatic amino acid transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine transporter TyrP"
                     /protein_id="HGU4550406.1"
                     /translation="MKNRTLGSVFIVAGTTIGAGMLAMPLAAAGVGFSVTLILLIGLW
                     ALMCYTALLLLEVYQHVPADTGLGTLAKRYLGRYGQWLTGFSMMFLMYALTAAYISGA
                     GELLASSISDWTGISMSATAGVLLFTFVAGGVVCVGTSLVDLFNRFLFSAKIIFLVVM
                     LVLLLPHIHKVNLLTLPLQQGLALSAIPVIFTSFGFHGSVPSIVSYMDGNVRKLRWVF
                     ITGSAIPLVAYIFWQVATLGSIDSTTFMGLLANHAGLNGLLQALREMVASPHVELAVH
                     LFADLALATSFLGVALGLFDYLADLFQRSNTVGGRLQTGAITFLPPLAFALFYPRGFV
                     MALGYAGVALAVLALIIPSLLTWQSRKHNPQAGYRVKGGRPALVVVFLCGIAVIGVQF
                     LIAAGLLPEVG"
     gene            complement(2268..2933)
                     /gene="yecA"
                     /locus_tag="ACHOSC_RS23130"
                     /old_locus_tag="pgaptmp_004626"
     CDS             complement(2268..2933)
                     /gene="yecA"
                     /locus_tag="ACHOSC_RS23130"
                     /old_locus_tag="pgaptmp_004626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UPF0149 family protein YecA"
                     /protein_id="HGU4550407.1"
                     /translation="MKTGPLNESELEWLDDILTKYNTDHAILDVAELDGLLTAVLSSP
                     QEIEPEQWLVAVWGGADYVPRWASEKEMTRFMNLAFQHMADTAERLNEFPEQFEPLFG
                     LREVDGSELTIVEEWCFGYMRGVALSDWSTLPDSLKPALEAIALHGTEENFERVEKMS
                     PEAFEESVDAIRLAALDLHAYWMAHPQEKAVQQPIKAEEKPGRNDPCPCGSGKKFKQC
                     CLH"
BASE COUNT          705 a          762 c          738 g          856 t
ORIGIN      
        1 aacaaatata acctttgtgg agcactatca tgctgaaacc agaaatgatt gaaaaactta
       61 atgagcagat gaacctggaa ctgtactctt cactgcttta tcagcaaatg agcgcctggt
      121 gcagctatca taccttcgaa ggtgctgccg cgttcctgcg ccgtcacgcc caggaagaga
      181 tgacgcatat gcagcgtctg tttgattacc tgactgatac cggcaattta ccgcgtatta
      241 ataccgttga atctccgttt gctgaatatt cctcacttga tgaattattc caggaaacct
      301 ataaacacga acaattaatc acgcagaaaa ttaacgaact ggctcatgct gcaatgacca
      361 atcaggacta cccaacattt aatttcctgc aatggtatgt ttctgagcag catgaagaag
      421 agaaactgtt caaatcgatt attgataaat taagcctggc aggcaaaagc ggcgaaggtc
      481 tgtattttat cgacaaagaa ctctctaccc tcgacacaca aaactaatgc tctcaggcgg
      541 caggttttct gccgccgttt caaatcagtg acggcaaatc ttactctgat cggtcgaaaa
      601 accttcttct gaaggtataa atttgccttt tgctgccaga aacgctacca gctctcccgc
      661 agtcatccct tctgccgagc aggtgtgaaa acgtgcctgt tcaccaaaac gcgctttaat
      721 tgctgcttcc agactggcat gcgtatattg ctcgcctgat tcaatcatca tatttaacac
      781 ttcatgaccg tgaatagagt ccatcgtccc tcctcaaaaa aagcctagcg tagcgattgc
      841 cgcttatgaa gactttgcgc cagcgcagga ctgaatgctt tttattgtac atttatattt
      901 acaccatatg taacgtcggt ttgacgaagc agccgttatg ccttaacctg cgccgcagat
      961 atcactcata aagatcgtca ggacagaaga aagcgtgaaa aacagaaccc tgggaagtgt
     1021 ttttatcgtg gcgggaacca caattggcgc aggcatgctg gcaatgccgc tggctgcggc
     1081 cggtgttggt tttagcgtta cgttaatctt gttgattggg ctttgggcgt tgatgtgcta
     1141 cacggcgcta ttactgctgg aggtgtacca gcatgttccg gcagataccg gtcttggcac
     1201 gctggcaaaa cgctatctgg gacgctacgg tcaatggctg acgggcttca gtatgatgtt
     1261 cttaatgtat gctctgactg cggcatacat cagcggtgcc ggtgaattgt tggcctccag
     1321 catcagcgac tggacaggta tttcaatgtc ggcaaccgct ggcgtgctgt tgttcacttt
     1381 tgttgccggt ggcgtggttt gtgttggaac atcgctggtc gatttattta accgttttct
     1441 gttcagcgcc aaaattattt ttctagtggt gatgctggta ttgctgctac cgcatattca
     1501 caaagtgaat cttttaaccc tgccgttgca acaggggctg gctctgtcag caatcccggt
     1561 gatttttacc tcatttggtt ttcacggtag cgtgccgagt attgtcagct atatggatgg
     1621 caacgttcgt aagctacgct gggtgtttat aaccggtagt gcgatccccc tggtggcata
     1681 tattttctgg caggtggcaa cgcttggcag cattgattca acaaccttta tgggattgct
     1741 ggctaatcat gctggattaa acgggctgtt acaggcgtta cgcgaaatgg tggcctctcc
     1801 gcatgttgag ctggcagtgc atttatttgc tgatttagcc ctcgccacgt catttctcgg
     1861 cgttgcgtta ggcttatttg attatctggc tgatttattt cagcgttcaa ataccgttgg
     1921 tggacggttg caaactggtg caattacctt tctgccgccg ttggcgtttg cactgtttta
     1981 tccacgagga tttgtgatgg cgctgggtta cgccggtgtg gcgctggcgg tactggcatt
     2041 gattatccct tcgctgttga cctggcaaag cagaaagcac aatcctcagg cgggttaccg
     2101 ggtgaaaggt ggtcgtccgg cgctggtggt ggtgtttctc tgtggtattg ctgtgattgg
     2161 cgtgcaattt ttgattgcgg cagggttgtt accagaagtg gggtgatcag atagcctcaa
     2221 attccttatt gggtgccaga attaacgctg acacccaatt tgacctctta atgcaggcag
     2281 cactgcttaa atttcttacc actaccgcac gggcaaggat cgttacgccc cggtttctct
     2341 tctgctttga tcggttgctg aacagctttt tcctgcggat gcgccatcca gtacgcatgt
     2401 agatcaagcg ccgccagtcg aatggcatct acgctctctt caaacgcttc tggcgacatt
     2461 ttctctaccc gctcgaagtt ttcctcagta ccgtgcagcg caatcgcctc cagcgctggt
     2521 tttaacgaat cgggcaacgt tgaccagtca gaaagtgcca cgccccgcat atagccaaag
     2581 caccactcct caacaatcgt cagctcgctg ccatcaactt ctcgcaagcc gaataacggc
     2641 tcaaactgct ccgggaattc gttcagacgc tctgcggtat cggccatatg ttgaaaagcc
     2701 agattcataa agcgcgtcat ctctttctct gacgcccagc gcggcacata gtcagcccca
     2761 ccccacacgg caaccagcca ctgttccggt tcaatctctt gcggagaact caacaccgcc
     2821 gtcaataaac cgtccagctc cgccacatca aggatggcgt ggtcagtgtt gtatttggtc
     2881 agaatatcgt ccagccattc caactcactt tcgtttaacg gtcccgtttt catacgcttt
     2941 tccttgtgga tctcaactcg ccagcaccta tcttacatgc cggtccgtat cagagatact
     3001 ttttgagtgg ctttgctggt gattaaaaat tagggagggt gtaacgacaa gttgcaggca
     3061 c
//
LOCUS       DAZKHF020000363         3048 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2201S31241
            SAMN43267800-rid25106413.denovo.365, whole genome shotgun sequence.
ACCESSION   DAZKHF020000363 DAZKHF020000000
VERSION     DAZKHF020000363.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267800
            Sequence Read Archive: SRR30307263
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3048)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3048)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 3048)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350581.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350581.2
            Genome Coverage       :: 151x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:21:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,652
            CDSs (total)                      :: 5,557
            Genes (coding)                    :: 5,320
            CDSs (with protein)               :: 5,320
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 237
            CDSs (without protein)            :: 237
            Pseudo Genes (ambiguous residues) :: 0 of 237
            Pseudo Genes (frameshifted)       :: 102 of 237
            Pseudo Genes (incomplete)         :: 130 of 237
            Pseudo Genes (internal stop)      :: 52 of 237
            Pseudo Genes (multiple problems)  :: 41 of 237
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3048
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267800-rid25106413.denovo.365"
                     /serotype="O 26:H11"
                     /isolate="2201S31241"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-01"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            complement(<1..173)
                     /locus_tag="ACHOSC_RS23135"
                     /old_locus_tag="pgaptmp_004627"
     CDS             complement(<1..173)
                     /locus_tag="ACHOSC_RS23135"
                     /old_locus_tag="pgaptmp_004627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000104949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HGU4550408.1"
                     /translation="MSTKLTGYVWDGCAASGMKLSSVAIMARLADFSNDEGVCWPSIE
                     TIARQIGAGMSTV"
     gene            complement(163..342)
                     /locus_tag="ACHOSC_RS23140"
                     /old_locus_tag="pgaptmp_004628"
     CDS             complement(163..342)
                     /locus_tag="ACHOSC_RS23140"
                     /old_locus_tag="pgaptmp_004628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001761574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4222 domain-containing protein"
                     /protein_id="HGU4550409.1"
                     /translation="MRQVNRWFKDHYGVPVRVIRWEPETQRVIYLREGYEHECFSPLE
                     QFRRKFREIEVGHEH"
     gene            complement(518..1075)
                     /gene="ymfL"
                     /locus_tag="ACHOSC_RS23145"
                     /old_locus_tag="pgaptmp_004629"
     CDS             complement(518..1075)
                     /gene="ymfL"
                     /locus_tag="ACHOSC_RS23145"
                     /old_locus_tag="pgaptmp_004629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415665.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YmfL"
                     /protein_id="HGU4550410.1"
                     /translation="MGKYHWKVEKQPEWYVKAVRKTIAALPGGYAEAAEWLDVTENAL
                     FNRLRADGDQIFPLGWAMILQRAGGTHFIADAVAQSANGVFVSLPDVEDVDNADINQR
                     LLEVIEQIGSYSKQIRSAIEDGVVEPHEKTAINDELYLSISKLQEHAALVYKIFCVSE
                     SNDARECAAPGVVASIASGCGETNA"
     gene            complement(1119..1319)
                     /gene="ymfT"
                     /locus_tag="ACHOSC_RS23150"
                     /old_locus_tag="pgaptmp_004630"
     CDS             complement(1119..1319)
                     /gene="ymfT"
                     /locus_tag="ACHOSC_RS23150"
                     /old_locus_tag="pgaptmp_004630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415664.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdaS family helix-turn-helix protein"
                     /protein_id="HGU4550411.1"
                     /translation="MKAYWDSLTKEQQGELAGKVGSTPGYLRLVFNGYKKASFVLAKK
                     LEQCTSGAITKSDLRPDIYPKD"
     gene            1410..2084
                     /gene="ymfK"
                     /locus_tag="ACHOSC_RS23155"
                     /old_locus_tag="pgaptmp_004631"
     CDS             1410..2084
                     /gene="ymfK"
                     /locus_tag="ACHOSC_RS23155"
                     /old_locus_tag="pgaptmp_004631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LexA family protein"
                     /protein_id="HGU4550412.1"
                     /translation="MKTIHDIRRSNARKLRDGVGGNSSFATMIDREPTQTSRFMGDGA
                     TKNIGDSMARHIEKCFDLPVGWLDQEHQTTNITKKPDVSITNKQITLVPVISWVQAGA
                     WKEVGYSEVDLSTAETYPCPVPCGEMTYILRVIGDSMIDEYRPGDMIFVDPEVPACHG
                     DDVIALMHDTGETTFKRLIEDGTQRYLKALNPNWPEPYIKINGNCSIIGTVIFSGKPR
                     RYTIKA"
     gene            2753..>3048
                     /gene="yfdP"
                     /locus_tag="ACHOSC_RS23160"
                     /old_locus_tag="pgaptmp_004632"
     CDS             2753..>3048
                     /gene="yfdP"
                     /locus_tag="ACHOSC_RS23160"
                     /old_locus_tag="pgaptmp_004632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416860.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YfdP"
                     /protein_id="HGU4550413.1"
                     /translation="MASERSTDVQAFIGELDGGVFETKIGAVLSEVASGVMNTKTKGK
                     VSLNLEIEPFDENRVKIKHKLSYVRPTNRGKISEEDTTETPMYVNRGGRLTILQ"
BASE COUNT          816 a          763 c          712 g          757 t
ORIGIN      
        1 ctgacggtac tcatccccgc gccaatctga cgggcaatgg tttcaattga tggccagcac
       61 acaccttcgt cattactgaa atcagccagg cgggccataa ttgccacgct ggataatttc
      121 atgcctgacg ctgcgcaacc atcccataca tagccggtta atttagtgct catgaccgac
      181 ctctatttcc ctgaatttac gacgaaactg ttcgagcggg ctgaagcact catgctcata
      241 gccttcacgg aggtagataa cccgttgtgt ttccggctcc caacgaatga ctctgacggg
      301 cactccgtag tgatctttga accagcggtt aacttgtcgc aaaggactgt ctccttctgc
      361 cggttgaaat cccccacagc ccactctgca aagctgtggg ttacaatttc cctgtcacct
      421 ggtacattta ctgcatagca atactccacc ttcgcttttc cacccggtac aggaagcgca
      481 atcagttgcg agcgacggta gtgtgttgtt aaactgttca tgcgttagtt tctccacaac
      541 cagaagcaat cgacgccacg acgcccggag ctgcacactc gcgggcgtca ttactttctg
      601 aaacgcaaaa aattttgtag acaagtgctg catgctcctg cagcttcgaa attgagaggt
      661 acagctcgtc gttaattgct gtcttctcat gcggttccac tacaccgtct tcgattgccg
      721 aacgaatctg ttttgaataa ctgccgatct gttcaatgac ttccagtaaa cgctggttaa
      781 tatcggcatt gtccacatcc tcgacgtcag gaagagacac aaagacgcca tttgcagact
      841 gcgccacagc gtcagcaatg aagtgagtgc caccagcacg ttgtaaaatc attgcccatc
      901 ccagcgggaa aatctgatcg ccatcggcac gaaggcggtt aaataatgcg ttctctgtta
      961 catccagcca ctcagcagct tcagcgtaac cccccggcaa cgctgcgata gtttttctga
     1021 cagctttcac gtaccactca ggctgttttt ctactttcca gtgatactta cccacggtta
     1081 gcctcatcgt tctgtggtta aaaattgaag gtgttctgtt aatctttcgg atagatatcc
     1141 ggtcttaagt cagatttcgt aattgcacct gacgtgcatt gctcaagttt tttcgccagc
     1201 acaaaactgg cttttttata accattgaaa accagccgta agtagccagg tgttgagcca
     1261 acttttccgg ccaactcgcc ctgctgttct ttggttaaag agtcccaata cgctttcata
     1321 caacatgtac ctccggtata catattacat gattgaaatg aaccttcaag atacttgtac
     1381 cttatcggta caaaggtttt aatttcgtta tgaaaacaat ccatgacatc cggcggtcta
     1441 acgccagaaa actgagagat ggtgttggcg gaaattcatc ctttgccacc atgattgatc
     1501 gcgagccaac ccaaaccagc aggtttatgg gggatggcgc tactaaaaat atcggtgaca
     1561 gcatggcacg ccacatcgaa aaatgtttcg acctgcctgt cggatggctt gatcaagaac
     1621 accagacaac gaacatcaca aaaaaacctg atgtttcaat cactaacaaa caaataacgt
     1681 tagtccctgt catatcatgg gtacaggccg gagcatggaa agaagttggc tattctgagg
     1741 ttgatttgag cacagcagaa acgtatccct gccctgtacc ctgtggcgaa atgacttata
     1801 tcttgcgggt gattggtgat tcaatgattg atgagtaccg ccctggagac atgatttttg
     1861 ttgatcccga agtccctgcc tgccacggtg acgacgttat tgcattgatg cacgatacag
     1921 gcgaaaccac cttcaagcga ttgatagaag atggaacaca gcgttatctc aaagcattaa
     1981 acccaaactg gcctgagcct tacattaaga ttaacggtaa ttgctctata attggtacag
     2041 tgattttctc gggaaaacca agaagataca caataaaggc ctaatcaata tttatgaacc
     2101 tgcttcggca ggttttttta tacttgacaa tgtacccatg agatacataa tgtatccaaa
     2161 agaaacatga ggcaggcaag attcaaacaa aatttggttg taacacggcg tatggcacat
     2221 gcgtcgttag cggtctgggg acgttaaagg ggacaatcca ctccttgcta gggcaaacaa
     2281 accaggtagc cggaatgtgc aagtcaatga tgatgctgat aagacgccta accagcgtgg
     2341 cgatccggtt tgacgcctgg gaagagacca gggtgcaacg atgagggcat ttatggagcc
     2401 gcgacaaagt gtggtgccgt aactggctaa gtgctctcag cgttgtggtc atccgcgaaa
     2461 tggcgcggcg gtaagtatgg cggggttact ctttccccgt tgaggacacc ggattgtcag
     2521 gttgaccata cgcctgagtg acaaccccac cacaacagcc actgctttgg cggtaccagt
     2581 ttgtacactt gcttccggct ggtaccgctc tttttacaaa acagagaaga gcatcaccgg
     2641 acgacgggct cataacccaa tccacccggg cggctgccac cgcaggtgct cttctctgtt
     2701 ttgtggagaa accaaccgac cttgcagggt cgatatgatg aggagcagca aaatggctag
     2761 cgaacgcagt actgatgtgc aggcatttat cggggagctg gacggcggcg tatttgaaac
     2821 caaaatcggc gctgttctca gtgaagtcgc ttcaggtgtg atgaacacga aaaccaaagg
     2881 gaaggtctcg ctcaacctgg aaatcgaacc gtttgatgag aaccgtgtga aaataaaaca
     2941 caaactctca tatgttcgtc cgactaaccg cgggaaaatt tccgaagaag acaccaccga
     3001 aacgccgatg tatgtcaatc gcggtggtcg cctgactatt ctgcagga
//
LOCUS       DAZKHF020000364         3033 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2201S31241
            SAMN43267800-rid25106413.denovo.366, whole genome shotgun sequence.
ACCESSION   DAZKHF020000364 DAZKHF020000000
VERSION     DAZKHF020000364.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267800
            Sequence Read Archive: SRR30307263
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3033)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3033)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 3033)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350581.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350581.2
            Genome Coverage       :: 151x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:21:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,652
            CDSs (total)                      :: 5,557
            Genes (coding)                    :: 5,320
            CDSs (with protein)               :: 5,320
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 237
            CDSs (without protein)            :: 237
            Pseudo Genes (ambiguous residues) :: 0 of 237
            Pseudo Genes (frameshifted)       :: 102 of 237
            Pseudo Genes (incomplete)         :: 130 of 237
            Pseudo Genes (internal stop)      :: 52 of 237
            Pseudo Genes (multiple problems)  :: 41 of 237
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3033
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267800-rid25106413.denovo.366"
                     /serotype="O 26:H11"
                     /isolate="2201S31241"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-01"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            complement(14..1873)
                     /gene="gss"
                     /locus_tag="ACHOSC_RS23165"
                     /old_locus_tag="pgaptmp_004633"
     CDS             complement(14..1873)
                     /gene="gss"
                     /locus_tag="ACHOSC_RS23165"
                     /old_locus_tag="pgaptmp_004633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional glutathionylspermidine
                     amidase/synthase"
                     /protein_id="HGU4550414.1"
                     /translation="MSKGTTSQDAPFGTLLGYAPGGVAIYSSDYSSLDPQEYEDDAVF
                     RSYIDDEYMGHKWQCVEFARRFLFLNYGVVFTDVGMAWEIFSLRFLREVVNDNILPLQ
                     AFPNGSPRAPVAGALLIWDKGGEFKDTGHVAIITQLHGNKVRIAEQNVIHSPLPQGQQ
                     WTRELEMVVENGCYTLKDTFDDTTILGWMIQTEDTEYSLPQPEIAGELLKISGARLEN
                     KGQFDGKWLDEKDPLQNAYVQANGQVINQDPYHYYTITESAEQELIKATNELHLMYLH
                     ATDKVLKDDNLLALFDIPKILWPRLRLSWQRRRHHMITGRMDFCMDERGLKVYEYNAD
                     SASCHTEAGLILERWAEQGYKGNGFNPAEGLINELAGAWKHSRARPFVHIMQDKDIEE
                     NYHAQFMEQALHQEGFETRILRGLDELGWDAAGQLIDGEGRLVNCVWKTWAWETAFDQ
                     IREVSDREFAAVPIRTGHPQNEVRLIDVLLRPEVLVFEPLWTVIPGNKAILPILWSLF
                     PHHRYLLDTDFTVNDELVKTGYAVKPIAGRCGSNINLVSHHEEVLDKTSGKFAEQKNI
                     YQQLWCLPKVDGKYIQVCTFTVGGNYGGTCLRGDESLVIKKESDIEPLIVVKK"
     gene            2078..2944
                     /gene="yghU"
                     /locus_tag="ACHOSC_RS23170"
                     /old_locus_tag="pgaptmp_004634"
     CDS             2078..2944
                     /gene="yghU"
                     /locus_tag="ACHOSC_RS23170"
                     /old_locus_tag="pgaptmp_004634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311901.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-dependent disulfide-bond
                     oxidoreductase"
                     /protein_id="HGU4550415.1"
                     /translation="MTDNTYQPAKVWTWDKSAGGAFANINRPVSGPTHEKTLPVGKHP
                     LQLYSLGTPNGQKVTIMLEELLALGVTGAEYDAWLIRIGDGDQFSSGFVEVNPNSKIP
                     ALRDHTHNPPIRVFESGSILLYLAEKFGYFLPQDLAKRTETMNWLFWLQGAAPFLGGG
                     FGHFYHYAPMKIEYAINRFTMEAKRLLDVLDKQLAQHKFIAGDEYTIADMAIWPWFGN
                     VVLGGVYDAAEFLDAGSYKHVQRWAKEVGERPAVKRGRIVNRTNGPLNEQLHERHDAS
                     DFETNTEDKRQG"
BASE COUNT          713 a          818 c          768 g          734 t
ORIGIN      
        1 gctacaacat gtattacttt ttcaccacaa ttaacggttc aatatcactc tcttttttga
       61 tgaccagtga ttcatcaccg cgcaaacacg tcccaccgta gttgccgcca acggtgaagg
      121 tacatacctg aatgtattta ccgtccactt tcggcaaaca ccacagttgc tgatagatgt
      181 ttttctgctc ggcaaattta ccgctggttt tgtccagcac ctcttcatga tggctgacca
      241 ggttgatatt gctaccacag cgaccggcaa tcggtttcac tgcgtaacct gttttcacca
      301 attcatcatt aacagtgaaa tcggtatcca gcaggtaacg atggtgcggg aacagcgacc
      361 agaggatcgg cagaatcgct ttgttgccgg ggatcaccgt ccacagcggc tcaaagacta
      421 gcacttccgg gcgcagcaat acgtcgataa gacgcacttc gttttgcgga tgaccggtac
      481 ggattggcac cgcagcaaac tcacggtcgc taacttcacg aatctgatca aacgcggttt
      541 cccacgccca ggttttccac acgcagttaa ccagccgtcc ttccccatca atcagttgcc
      601 cggcagcatc ccagcccagt tcatccagcc cgcgcaagat acgcgtttca aagccctcct
      661 ggtgcagcgc ctgctccata aactgcgcgt gatagttttc ctcgatatct ttgtcctgca
      721 tgatatggac aaacggacgt gcacgactgt gtttccaggc accagccagt tcgttaatca
      781 gcccttccgc cggattgaag ccgttgcctt tatagccctg ctccgcccaa cgttcgagga
      841 tcaagcccgc ttcggtatga catgaggcag agtcggcgtt gtattcgtaa accttcaggc
      901 cacgctcatc catgcagaaa tccatacgac cagtgatcat atggtgacgg cgacgctgcc
      961 aggagagacg caaacgtggc cagaggattt tcgggatgtc gaacagcgcc agcaggttgt
     1021 catctttcag caccttgtcg gttgcgtgaa gatacatcag gtgcagctcg ttggtggctt
     1081 taattagctc ctgctcggca ctctcggtaa tggtgtagta atgataagga tcctgattga
     1141 tcacctgacc gttggcctgc acataggcgt tttgcagcgg atctttttca tccagccatt
     1201 taccgtcaaa ctggcctttg ttttccaggc gcgctccgct gattttcagc agctcgcctg
     1261 caatttccgg ctgcggtaag ctgtattcag tatcttccgt ctggatcatc cagcccagaa
     1321 tggtggtgtc atcaaaagtg tctttcaggg tatagcagcc gttttcgacc accatctcca
     1381 gctcacgcgt ccactgttgc ccttgcggca acggggaatg aatcacgttc tgttccgcaa
     1441 tacggacttt gttgccatgc aattgggtaa tgatggcgac atggccagtg tctttaaatt
     1501 caccgccttt atcccagata agaagcgcac ccgcgaccgg cgcacgcggc gagccgttag
     1561 gaaatgcctg caatggcagg atgttgtcat taaccacttc tcgcaggaag cgcagcgaga
     1621 aaatctccca cgccataccc acgtcagtaa agaccacacc gtaattcaga aagagaaaac
     1681 ggcgagcaaa ttcaacgcat tgccacttgt ggcccatata ttcgtcgtcg atatagctac
     1741 ggaatacggc gtcatcttcg tattcctgcg gatcgagaga actgtaatct gaagagtaga
     1801 ttgctacccc acctggggcg tagcccaata atgtcccgaa cggggcatcc tggctggtcg
     1861 ttcctttgct catcacttta cctttaacaa tacaacctaa gcagttggcg taattgttgt
     1921 gctctgatta cctgctcagc gacattaacc ggacagaggt cagactaatc atacacctca
     1981 tcgcagcggc tggcgcaaag ggttaaaaaa ttcacattct gtacagcaag tgacctgcta
     2041 cactgcttca acactaccac tcagaaggca actcactatg acagacaata cttatcagcc
     2101 cgcgaaagtc tggacgtggg ataaatccgc tggcggcgcg ttcgccaata tcaatcgccc
     2161 ggtttctggt ccgacgcatg aaaaaacgct gcccgttggc aaacacccat tgcaacttta
     2221 ttcgctggga acgccgaacg gtcagaaagt aacgattatg cttgaggagc tgctggcgct
     2281 gggcgttact ggtgcagagt acgacgcctg gctgattcgt attggcgatg gcgatcaatt
     2341 ctccagcggc tttgtcgaag tgaacccaaa ctcgaagatc ccggcgctgc gcgatcatac
     2401 gcataatccg ccgatccgcg tgtttgaatc tggttcgatc ctgctttatc tggcggagaa
     2461 atttggctac ttcctgccgc aggatttggc aaagcgtact gaaacgatga actggctgtt
     2521 ctggttacag ggcgcggcac cgttcctcgg cggtggtttt ggtcactttt accattacgc
     2581 gccgatgaaa attgagtacg ccatcaaccg ctttaccatg gaagccaaac gtctgctcga
     2641 cgtgctggat aagcaactgg cgcagcataa gtttattgcg ggcgatgagt acaccattgc
     2701 ggatatggcg atttggccgt ggtttggcaa tgtggtgtta ggcggcgtgt atgatgccgc
     2761 agagtttctt gatgcgggca gttataagca tgtacaacgc tgggcgaaag aagtaggcga
     2821 acgtccggcg gtgaaacgtg ggcgtattgt taaccgcacc aacggaccgc tgaatgagca
     2881 gttgcatgag cgccatgacg ccagtgattt cgagacgaat acggaagata agcgtcaggg
     2941 gtaagggttg gtgttcgtcg cagcaagcca tccaggccgg ataaggcgtt cacgccgcat
     3001 ccggcaatcg tgcacaatgc ctgatgcgac gct
//
LOCUS       DAZKHF020000365         3019 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2201S31241
            SAMN43267800-rid25106413.denovo.367, whole genome shotgun sequence.
ACCESSION   DAZKHF020000365 DAZKHF020000000
VERSION     DAZKHF020000365.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267800
            Sequence Read Archive: SRR30307263
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3019)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3019)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 3019)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350581.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350581.2
            Genome Coverage       :: 151x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:21:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,652
            CDSs (total)                      :: 5,557
            Genes (coding)                    :: 5,320
            CDSs (with protein)               :: 5,320
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 237
            CDSs (without protein)            :: 237
            Pseudo Genes (ambiguous residues) :: 0 of 237
            Pseudo Genes (frameshifted)       :: 102 of 237
            Pseudo Genes (incomplete)         :: 130 of 237
            Pseudo Genes (internal stop)      :: 52 of 237
            Pseudo Genes (multiple problems)  :: 41 of 237
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3019
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267800-rid25106413.denovo.367"
                     /serotype="O 26:H11"
                     /isolate="2201S31241"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-01"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            complement(<1..290)
                     /locus_tag="ACHOSC_RS23175"
                     /old_locus_tag="pgaptmp_004635"
     CDS             complement(<1..290)
                     /locus_tag="ACHOSC_RS23175"
                     /old_locus_tag="pgaptmp_004635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000448925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4550416.1"
                     /translation="METLFKVFEKFSSRPLFFIFFGLSLCEFFQKQSVLMNPSADNIA
                     KLFAAMILVVFFTWGFEWLIFKFNVNLEPHDQGDIGPTIGTATLAVYLVYAF"
     gene            complement(329..1558)
                     /locus_tag="ACHOSC_RS23180"
                     /old_locus_tag="pgaptmp_004636"
     CDS             complement(329..1558)
                     /locus_tag="ACHOSC_RS23180"
                     /old_locus_tag="pgaptmp_004636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001341819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="integrase domain-containing protein"
                     /protein_id="HGU4550417.1"
                     /translation="MAKIAKKLTDTEIKSTKPADKEINLFDGDGLILRIAPLAKGGKK
                     NWYFRYAVPVSKKRTKMSLGTYPHLTLARARALRDEYLSFLANGVDPQIHNNDKAKAL
                     KSATEHTLQAVARKWLDEKVKTSGISQDHAADIWRSLERNVFPGLGNVPINEIRPKLL
                     KQHLDPIEQRGVLETLRRIISRLNEIFRWAATEELIEFNPADNLGQRFSKPKKQNMPA
                     LPPSELPRFMESLTNASIRLETRMLIEWQLLTWVRPGEAVRARWSDIDTTNSIWNIPA
                     DFMKMKKLHKVPLSKEALRILELMKSISGHREWVFPSIKAPLNHMHEQTANAAIIRMG
                     FGGELVAHGMRSIARTAAEESGKFRAEVLEAALAHSKKDEIIAAYNRAEYLIERQSLM
                     QWWSDYVQAQRSNALVA"
     gene            complement(1857..2765)
                     /locus_tag="ACHOSC_RS23185"
                     /old_locus_tag="pgaptmp_004637"
     CDS             complement(1857..2765)
                     /locus_tag="ACHOSC_RS23185"
                     /old_locus_tag="pgaptmp_004637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001431537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4760 domain-containing protein"
                     /protein_id="HGU4550418.1"
                     /translation="MARTLVNVSATIFALMMIVRALFTYIYPGKLPFSIAIIDWLIVI
                     AGSGAAISSIFCFIKKRYPDTAEFLPMFSTVCYVIILIGYAILRYTPAYQTSLSIMVT
                     GMLVGMGWWIQCITSAANTRRSHTLNMIINTRTSPEYQKQLRNSTKFYRGMRYVPQEL
                     SEWRCNPDKEEYKNMKVPDEYRDAINGLLYILNYFEFLAQGIKFKDLDDELLRECFSS
                     FLRGIERRGFHMILESQKQDPAAFEGIIYLSKKWNGTSFVETHRSNPNTVELGVPYPS
                     NDMVEKMVQGQPLIDSDAGPELQVAT"
BASE COUNT          806 a          647 c          651 g          915 t
ORIGIN      
        1 tgaaaggcat aaactaagta tacagctaaa gtagccgttc caattgttgg tccaatatcg
       61 ccttgatcat gaggttcaag gtttacattg aacttgaaga ttagccattc aaatccccaa
      121 gtaaaaaaaa caactaatat catggctgcg aataatttcg cgatgttatc tgctgatgga
      181 ttcatcagaa cagattgttt ctgaaaaaat tcacaaagtg agagtccgaa aaaaataaaa
      241 aaaagtggtc tggaactaaa tttttcaaaa actttgaata gggtttccat tttctttacc
      301 ttcaggatta tattagctat tctgatactt aggctaccag agcatttgat ctttgagctt
      361 gaacgtaatc actccaccat tgcatcaaac tctgtcgctc tatcagatat tctgcacgat
      421 tgtatgctgc gataatttca tcttttttcg agtgggcaag cgctgcctca agaacttcag
      481 ctctgaattt accagactcc tctgccgctg ttcgtgcaat agaacgcata ccgtgagcta
      541 caagctcgcc tccgaacccc attcggatga tagctgcgtt ggctgtttgt tcatgcatat
      601 gattaagagg cgcttttatg ctggggaaaa cccattctct atgcccactt attgatttca
      661 ttaattcaag gatgcgcaaa gcttctttac tcaaaggaac tttgtgaagc tttttcattt
      721 tcatgaaatc agcaggaatg ttccaaatgc tgttggttgt atcaatatca gaccaccttg
      781 cgcgaacggc ttcacccgga cgaacccatg tcaacaattg ccattcaatt agcatacgtg
      841 tttccaaccg gattgacgca ttcgtcaaag attccataaa ccttggcaat tcgcttgggg
      901 gaagggcagg catattttgc ttttttggtt tactgaatct ttgaccaagg ttgtcagccg
      961 ggttgaactc aataagttct tcagtagctg cccaccggaa gatttcattc agacgtgaaa
     1021 tgatacggcg tagagtttcc aatacgcctc gttgctcaat aggatcaagg tgttgtttta
     1081 agagcttagg tcggatctca ttgataggga cattacccag accgggaaag acatttctct
     1141 ctaagctgcg ccagatgtct gctgcatggt cttgtgagat acctgatgtc tttaccttct
     1201 catctaacca tttccgcgct acggcttgga gagtgtgctc agtagcactc tttaatgcct
     1261 tcgccttatc gttgttatgg atttggggat caacaccatt tgccagaaag gagagatatt
     1321 catcacgtaa ggctctggct cttgcaaggg taaggtgagg atatgtccca aggctcattt
     1381 tggttctttt cttgctcacg ggtactgcat acctgaaata ccaatttttc ttgcctcctt
     1441 tcgccaaagg agcgattcgt agaatcagac catcaccgtc aaacaagttg atttctttat
     1501 cggctggctt ggtgcttttg atttcagtgt cagtgagctt cttagcgatt tttgccattt
     1561 tgggaccctc ggtttttgga cccttcttag tgggtcccat tcagggtgcc ataactcgta
     1621 gttctcagca attctcactg gacgacaata gacgtaaaaa agcccgcaga gcttgtgctg
     1681 tgtgggctta gtagacttca ttgaacttca aacaactaaa aagtggagct ggcgggagtt
     1741 gaacccgtgc ccgagcaatg cttaacccat tgaataacaa tgcattctct gtttaaaaat
     1801 gcctcaagtg cattttgagt gcattttctg gtcctctctt ggtccataca acgagattaa
     1861 gtggccactt gaagttccgg ccctgcgtcg ctatctatta acggctgacc ttgaaccatt
     1921 ttttcaacca tatcgtttga aggatatgga accccgagct caaccgtatt agggttggaa
     1981 cggtgggttt caacaaacga tgttccgttc catttttttg aaaggtagat tattccttca
     2041 aaggctgcag ggtcctgttt ctgagactct aagatcatgt ggaagccacg acgttctatc
     2101 ccgcgtaaga agctggagaa gcactccctc agtaattcgt catccaaatc cttgaacttg
     2161 ataccctgcg caagaaactc gaaataatta agaatatata aaaggccatt gatggcatcc
     2221 ctgtactcat cggggacctt catattcttg tattcttctt tgtctgggtt gcagcgccac
     2281 tctgatagtt cttgtggtac ataacgcatg ccccgataga atttggtgct gttccgaagc
     2341 tgtttttggt actccgggct tgttcgggtg ttaatgatca tgttcagcgt gtgggacctg
     2401 cgcgtgttag cagcggacgt tatgcattgt atccaccatc ccattccaac gagcattcct
     2461 gtaaccatga tcgaaagcga tgtttgatag gccggtgtgt accttaaaat tgcatatcca
     2521 ataaggataa tcacataaca aacagtgctg aacataggaa gaaattctgc agtgtctgga
     2581 tagcgttttt tgatgaagca gaagatagaa gatattgctg caccagaacc agcaatgacg
     2641 atcagccaat caatgatggc gatgctaaat ggtagttttc cagggtagat ataagtaaaa
     2701 agtgctctga caatcatcat tagagcaaaa attgtagcgc tgacgttgac taatgttctc
     2761 gccatcttat tgattcctat aacaaaagac ctccgcatgg gaggccttat tgtgctaatg
     2821 gaggtttgtt ttaaaatttt aggttgtagt taaccttcac cgaaacctac ggtcagtgtc
     2881 tttttcatag tgttcactcc agttgtggtg cttcgccgat acctacgtta agctcttgaa
     2941 aagtaaaaaa acataaaagt tttagaattt gtggagcaca ggataacagt ttgtactggg
     3001 actcatcaag tactatcag
//
LOCUS       DAZKHF020000366         2997 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2201S31241
            SAMN43267800-rid25106413.denovo.368, whole genome shotgun sequence.
ACCESSION   DAZKHF020000366 DAZKHF020000000
VERSION     DAZKHF020000366.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267800
            Sequence Read Archive: SRR30307263
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2997)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2997)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 2997)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350581.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350581.2
            Genome Coverage       :: 151x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:21:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,652
            CDSs (total)                      :: 5,557
            Genes (coding)                    :: 5,320
            CDSs (with protein)               :: 5,320
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 237
            CDSs (without protein)            :: 237
            Pseudo Genes (ambiguous residues) :: 0 of 237
            Pseudo Genes (frameshifted)       :: 102 of 237
            Pseudo Genes (incomplete)         :: 130 of 237
            Pseudo Genes (internal stop)      :: 52 of 237
            Pseudo Genes (multiple problems)  :: 41 of 237
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2997
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267800-rid25106413.denovo.368"
                     /serotype="O 26:H11"
                     /isolate="2201S31241"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-01"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            <1..305
                     /locus_tag="ACHOSC_RS23190"
                     /old_locus_tag="pgaptmp_004638"
     CDS             <1..305
                     /locus_tag="ACHOSC_RS23190"
                     /old_locus_tag="pgaptmp_004638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000242972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="porin"
                     /protein_id="HGU4550419.1"
                     /translation="EPIIKYAFENAGNLYLRTRFGHGKTENADSLQYNANRTYFKTIR
                     KATLGYEQKIGDDWIAKIEYKHAWEKEHKNFYDPRDEEKYNVLNQDNIYVHAFYRF"
     gene            324..1280
                     /locus_tag="ACHOSC_RS23195"
                     /old_locus_tag="pgaptmp_004639"
     CDS             324..1280
                     /locus_tag="ACHOSC_RS23195"
                     /old_locus_tag="pgaptmp_004639"
                     /EC_number="2.3.1.191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000490374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016410 - N-acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-3-O-(3-hydroxymyristoyl)glucosamine
                     N-acyltransferase"
                     /protein_id="HGU4550420.1"
                     /translation="MFSALDIKTLMQGTLYGDPSLRVDTIRPIHSPLVGGLSIVMTPG
                     DLLHIPTTGADIIIGPEEIISSNAKAKIVVDYLNVNNLNKVLRYFKVHKYRLFEQENT
                     STIPDVYIGKHCQIGMNCHFMPGVKIMNCVTIGDNVAIHANTVIKEGTIIGNDVIIDS
                     NNSIGNYSFEYMSDERDSYVRVDSIGRVIIGDDVEIGCNNTIDRGTLGDTIIGQGTRI
                     DNQVQIGHDCIIGNKCLIVSQCGFSGHVVLGDHVITHGQVGIAGHISIGSYSVIKAKS
                     GVSHSCPEKSDLFGYPAKNTREYNKNLAVLNKLTKQHGVYKQ"
     gene            1371..1859
                     /locus_tag="ACHOSC_RS23200"
                     /old_locus_tag="pgaptmp_004640"
     CDS             1371..1859
                     /locus_tag="ACHOSC_RS23200"
                     /old_locus_tag="pgaptmp_004640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001461083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosidase"
                     /protein_id="HGU4550421.1"
                     /translation="MKNKLLIAAVLTGLLSGCVDGNSPSGSQEGVVKADPRYSECNLP
                     DVNERGPIAAGLYISGTLPEGYWLMKPERKMHYKGDGIYQLIYSEKAGAVTMQFATMN
                     WNPQFTVTGLELPLNQAKTLKKGGMDKNTKVVIPADGKYVWTIQIGPDKKAIAAMVAP
                     CK"
     gene            1942..>2997
                     /locus_tag="ACHOSC_RS23205"
                     /old_locus_tag="pgaptmp_004641"
     CDS             1942..>2997
                     /locus_tag="ACHOSC_RS23205"
                     /old_locus_tag="pgaptmp_004641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000741720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HGU4550422.1"
                     /translation="MAAVILRKVEKIYDNGFKAVHGIDLDINDGEFMVFVGPSGCAKS
                     TTLRMIAGLEDVSGGEIYISDRLVNTLPPKDRSIAMVFQNYALYPHKTVFDNMAFGLK
                     MQKRPKEEIKKIVEEVAEKLEISDLLYRKPKEMSGGQRQRVAVGRAIVRKPDVFLFDE
                     PLSNLDAKLRVSMRVKIAQLHQSLKDEGHPATMIYVTHDQIEALTLGDRICVLNQGRI
                     MQVDTPVNLYNHPANRFVAGFIGSPSMNLIDAVVREEDGKLYIDIAKNIRLYIPENKQ
                     SVLKAYIDKPVCFGIRPEHISLSLNCKEMNTVTGALSIIENMGSEKYLYFTVNNKEFI
                     AKVNDQSITTADIGKNLY"
BASE COUNT          970 a          527 c          629 g          871 t
ORIGIN      
        1 acgagccaat tatcaaatat gcttttgaaa acgcaggtaa tctgtatcta cgcacccgct
       61 tcggacacgg gaaaacagaa aacgcagata gtttgcaata caatgcaaac agaacctatt
      121 tcaaaacaat tcgaaaagcc actctaggtt atgaacaaaa aataggtgac gactggatag
      181 ccaagattga atataaacat gcctgggaaa aagagcacaa aaacttttat gatccaagag
      241 atgaagaaaa atataatgtg ctcaatcagg ataatattta tgttcatgct ttctatcgct
      301 tttgattaaa cagagggaaa cctatgttca gcgcactaga tattaaaacg ttgatgcaag
      361 gaactttata tggtgatcca tcactcaggg ttgataccat tcggccaatc catagtcctt
      421 tagttggtgg actctcgatt gttatgacac ctggagattt gttgcatata ccaacgactg
      481 gtgcggatat aattatcgga ccggaagaga tcattagttc taatgctaag gcaaagattg
      541 tagtcgatta cctgaacgtg aataatctta acaaagtttt aagatatttt aaggtacata
      601 agtatcgttt gtttgagcaa gaaaacactt caacgatacc tgatgtatac attggtaaac
      661 actgccagat aggtatgaac tgccatttta tgcctggtgt taaaattatg aactgtgtga
      721 ccataggtga taatgttgcc attcatgcga atacagtgat taaagaaggt actattattg
      781 gcaatgacgt tatcatagat tctaataact ccatcggtaa ttatagtttt gaatatatgt
      841 cggatgaacg tgattcatat gtccgagttg atagcatcgg tagagtaatc ataggtgatg
      901 atgttgaaat cggttgtaac aacaccattg accgagggac acttggagat acgattatag
      961 gccaaggcac tcgtattgat aatcaagtgc agattggaca tgactgcata atcggtaata
     1021 aatgcctgat tgtttcacaa tgcggttttt caggacacgt agttttaggc gaccatgtta
     1081 ttacacatgg tcaagtcgga atcgccggtc acatatcaat tggtagttat tcggtaatta
     1141 aggctaaatc aggtgtaagc cattcttgtc ctgaaaagag tgaccttttt ggttaccctg
     1201 ccaaaaatac gcgagaatac aacaaaaatc tcgctgtcct taataaacta actaaacagc
     1261 atggtgtata taaacagtga gtcgttgaga agtattattt atatacatta acattaacac
     1321 tgacatagaa atagcttaac taagctatac tacttttatt gagagtttaa atgaaaaata
     1381 aattgcttat tgcggctgtg ttgactggtc ttctttcagg atgcgttgat ggcaattcac
     1441 cctctggcag tcaggaaggt gtcgtgaaag cggatcctcg ctattcagaa tgtaacctcc
     1501 ctgatgtaaa tgaaagaggg ccaattgctg cgggtcttta catttccgga accctcccgg
     1561 aaggctattg gttaatgaag ccagagcgta aaatgcatta taagggagat ggtatttatc
     1621 agcttattta ttctgaaaaa gcaggtgcgg taacaatgca atttgcgact atgaactgga
     1681 acccgcagtt cactgtaaca ggtcttgaat tgccattaaa tcaggctaaa acactcaaga
     1741 aaggaggcat ggataaaaat acaaaagtcg ttattcctgc cgatggaaag tatgtttgga
     1801 cgattcaaat tggtcctgat aaaaaggcaa tagccgccat ggtagcacca tgtaaatagt
     1861 tatttttagc actccactat tcaacattcg ttgaatagtg gagtatcagt attccaaaat
     1921 tcttgttaat gagagcagat tatggccgcg gtaattctac gcaaagttga aaaaatttat
     1981 gacaatggat tcaaagctgt tcacggcatt gatctcgata taaacgatgg tgaattcatg
     2041 gtttttgttg gaccatctgg gtgtgccaaa tcaactacat tacgcatgat tgcaggtctt
     2101 gaagacgtat caggtggtga aatttacatt agcgaccgcc ttgttaatac ccttccgcct
     2161 aaagatcgca gcattgcaat ggtgttccag aactatgcgc tgtatcccca taagactgta
     2221 tttgacaata tggcttttgg tttaaaaatg caaaaaaggc caaaagaaga gattaagaaa
     2281 attgtcgaag aagttgcaga aaaacttgaa attagcgatt tactttaccg aaaaccgaaa
     2341 gaaatgtcgg gtggtcagcg tcagcgtgtc gcggtaggcc gtgcaattgt ccgtaagcca
     2401 gatgttttct tatttgatga gccactatct aaccttgatg caaaacttcg tgtttcaatg
     2461 cgagtcaaaa ttgctcagtt gcatcagtct ctcaaggatg agggtcatcc agcaaccatg
     2521 atttatgtta cacacgatca aattgaagca ttgaccctcg gtgatcgtat ttgtgtgctt
     2581 aatcaggggc gaataatgca ggtcgacaca ccagttaatc tgtataacca ccctgctaac
     2641 agattcgttg ctggctttat tggttccccg tcaatgaatt tgatagatgc ggttgtacgt
     2701 gaggaagacg gtaaactcta tattgacatt gccaaaaata ttcggttgta catacctgag
     2761 aataaacaat cagtactcaa agcgtatata gataaacccg tttgttttgg tattcgtcca
     2821 gaacatatct ctttgagttt gaattgcaaa gaaatgaata cagtcactgg tgcattaagt
     2881 attattgaaa atatgggcag tgaaaaatat ctttacttta ccgttaacaa caaagagttt
     2941 attgcaaaag tcaacgatca atcaattact acagccgata ttggtaaaaa tttgtat
//
LOCUS       DAZKHF020000367         2986 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2201S31241
            SAMN43267800-rid25106413.denovo.369, whole genome shotgun sequence.
ACCESSION   DAZKHF020000367 DAZKHF020000000
VERSION     DAZKHF020000367.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267800
            Sequence Read Archive: SRR30307263
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2986)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2986)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 2986)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350581.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350581.2
            Genome Coverage       :: 151x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:21:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,652
            CDSs (total)                      :: 5,557
            Genes (coding)                    :: 5,320
            CDSs (with protein)               :: 5,320
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 237
            CDSs (without protein)            :: 237
            Pseudo Genes (ambiguous residues) :: 0 of 237
            Pseudo Genes (frameshifted)       :: 102 of 237
            Pseudo Genes (incomplete)         :: 130 of 237
            Pseudo Genes (internal stop)      :: 52 of 237
            Pseudo Genes (multiple problems)  :: 41 of 237
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2986
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267800-rid25106413.denovo.369"
                     /serotype="O 26:H11"
                     /isolate="2201S31241"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-01"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            381..947
                     /locus_tag="ACHOSC_RS23210"
                     /old_locus_tag="pgaptmp_004642"
     CDS             381..947
                     /locus_tag="ACHOSC_RS23210"
                     /old_locus_tag="pgaptmp_004642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HGU4550423.1"
                     /translation="MAKPARRRCNRKREDLTVKRIFELLSFDKSTGVFRWKVPTQGRI
                     ALNSVAGTFDSNGYSMIMIDGRRYKTHVLVFYITHNRWPAGQIDHVNGIRTDNRPENL
                     RECLPIENSRNIRIRKNSKSGCRGVTWHKRQKKWNVRLGFHGKSKHFGCFDDLELAVL
                     VAEEARDKYYGDFSGNERSTYANLSKEM"
     gene            922..1524
                     /locus_tag="ACHOSC_RS23215"
                     /old_locus_tag="pgaptmp_004643"
     CDS             922..1524
                     /locus_tag="ACHOSC_RS23215"
                     /old_locus_tag="pgaptmp_004643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination protein NinG"
                     /protein_id="HGU4550424.1"
                     /translation="MRIYRRKCKCCNEWFIPKYQNQYWCNEICGTKIALERRSKEREK
                     AEKAAEKKLRREEQKQKDKLKIRKLALKPRSYWIKQAQQAVNAFIRERDRDLPCISCG
                     TLTSAQWDAGHYRTTAAAPQLRFDERNIHKQCVVCNQHKSGNLVPYRVELISRIGQEA
                     VEEIESNHNRYRWTVEECRAIKAEYQQKLKKLRNSRSEVA"
     gene            1521..2186
                     /locus_tag="ACHOSC_RS23220"
                     /old_locus_tag="pgaptmp_004644"
     CDS             1521..2186
                     /locus_tag="ACHOSC_RS23220"
                     /old_locus_tag="pgaptmp_004644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine protein phosphatase"
                     /protein_id="HGU4550425.1"
                     /translation="MNIYERIDGSKYRNIWVVGDLHGCYTNLMKKLETIGFDTKKDLL
                     ISVGDLVDRGTENVECLELITFPWFRAVRGNHEQMMIDGLSERGNVNHWLLNGGGWFF
                     NLDYDKEILAKALAHKADELPLIIELVSKGKKYVICHADYPCDKYEFGKPVDHQQVIW
                     NRERISNSQDGIVKEIKGADTFIFGHTPAVKPLKFANQMYIDTGAVFCGNLTLIQVQG
                     EGA"
     gene            2183..2806
                     /locus_tag="ACHOSC_RS23225"
                     /old_locus_tag="pgaptmp_004645"
     CDS             2183..2806
                     /locus_tag="ACHOSC_RS23225"
                     /old_locus_tag="pgaptmp_004645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="antitermination protein"
                     /protein_id="HGU4550426.1"
                     /translation="MRLESVAKFHSPKSPMMSDSPRATASDSLSGTDVMAAMGMAQSQ
                     AGFGMAAFCGKHELSQNDKQKAINYLMQFAHKVSGKYRGVAKLEGNTKAKVLQVLATF
                     AYADYCRSAATPGARCRDCHGTGRAVDIAKTEQWGIVAEKECGRCKGVGYSRMPASAA
                     YRAVTMLIPNLTQPTWSRTVKPLYDALVVQCHKEESIADNILNAITR"
BASE COUNT          920 a          593 c          728 g          745 t
ORIGIN      
        1 tcgattttag atttttccag tatttgtaga tattgcactg aacaccgaat acgtagcaga
       61 gggtgtctac acgataacgt tctatgagct accatgttgt cgaaaaattg tttagtgagt
      121 atgacatcag gaatgtggtg gtctgtttta atatttctat ttctatttct atttctattt
      181 ctgtttgttg tggttttttc tttagttcat gtatttcgat ttgtttctga attatcgggg
      241 gtagtgtctt cccttttccc tgaggcttat catgcagtta ttttcgccat cttggtattg
      301 tggaaatacc gacatccata gctttggtga gctctgttca ggtggccaaa ttcagaaaac
      361 cattacggag gaagaaggcg atggctaaac cagcgcgaag acgatgtaac cgtaaaagag
      421 aagatttaac tgttaaaagg atatttgagt tactaagttt cgataaatct accggggtat
      481 ttagatggaa agttcccact cagggaagga tagcattaaa tagtgttgct ggaacttttg
      541 attccaacgg ttattcaatg atcatgatag atgggcgtag atataaaact cacgtcttag
      601 ttttttacat aactcataat cgttggcctg ctggtcaaat tgaccacgtt aatggaatta
      661 ggaccgacaa taggccagaa aatttaagag aatgcctgcc aatagaaaat tcaagaaata
      721 taaggatccg aaagaatagc aaatcaggtt gcagaggggt tacttggcac aaacgacaga
      781 aaaaatggaa tgttaggcta ggattccatg gcaagagtaa acacttcgga tgctttgatg
      841 atctggagtt agcggtacta gttgctgaag aagcccgaga taagtattac ggtgattttt
      901 ccggcaacga aaggagcact tatgcgaatc tatcgaagga aatgtaaatg ttgcaatgaa
      961 tggtttatac caaaatatca aaatcaatat tggtgtaatg agatttgtgg aaccaagata
     1021 gcactcgaac gacgaagtaa agaacgcgaa aaagcggaaa aagcagcaga gaagaaacta
     1081 cgacgagagg agcagaaaca gaaagataaa ctgaagattc gaaaactcgc cttaaagccc
     1141 cgcagttact ggattaaaca agcccaacaa gccgtaaacg ccttcatcag agaaagagac
     1201 cgcgacttac catgtatctc gtgcggaacg ctcacgtctg ctcagtggga tgccggacat
     1261 taccggacaa ctgctgcggc acctcaactc cgatttgatg aacgcaatat tcacaagcaa
     1321 tgcgtggtgt gcaaccagca caaaagcgga aatctcgttc cgtatcgcgt cgaactgatt
     1381 agccgcatcg ggcaggaagc agtagaggaa atcgaatcaa accataaccg ctatcgctgg
     1441 actgtcgaag agtgcagggc catcaaggcg gagtatcaac agaaacttaa aaaactgcga
     1501 aacagcagaa gtgaggttgc atgaatatct acgaaagaat tgatggcagc aaataccgaa
     1561 atatttgggt agttggcgat ctgcacggat gctacacgaa cctgatgaaa aaactggaga
     1621 cgataggatt cgacaccaaa aaagacctgc ttatctcggt gggcgatttg gttgatcgcg
     1681 gtacagagaa cgtagaatgc ctggaattaa tcacattccc ctggttcaga gctgtacgtg
     1741 gaaaccatga gcaaatgatg attgatggct tatcagagcg tggaaacgtc aatcactggc
     1801 tgcttaatgg cggtggctgg ttctttaatc tcgattacga caaagaaatt ctggctaaag
     1861 ctcttgccca taaagcagat gaacttccgt taatcatcga actggtgagt aaaggaaaaa
     1921 aatatgtcat ctgccacgcc gattatcctt gtgataaata cgagtttgga aagccagttg
     1981 atcatcagca ggtaatctgg aaccgcgaac gaatcagcaa ctcacaagac gggatcgtga
     2041 aagaaatcaa aggcgcggac acgttcatct ttggtcatac gccagcagtg aaaccactca
     2101 aatttgccaa ccagatgtat atcgatactg gcgcagtgtt ctgcggaaac ctcacattga
     2161 ttcaggtaca gggagaaggc gcatgagact cgaaagcgta gctaaatttc attcgccaaa
     2221 aagcccgatg atgagcgatt caccacgggc tacggcttct gactctcttt ctggtactga
     2281 tgtgatggct gctatgggga tggcgcaatc acaagccgga ttcggaatgg ctgcattctg
     2341 cggtaagcat gaactcagcc agaacgacaa acaaaaggct atcaactatc tgatgcaatt
     2401 tgcacacaag gtatcgggga aataccgtgg tgtggcaaag ctcgaaggaa atactaaggc
     2461 aaaggtactg caagtgctcg caacattcgc ttatgcggat tattgccgta gtgccgctac
     2521 gccgggcgca agatgcagag attgccacgg tacaggccgt gcggttgata tagccaaaac
     2581 agagcagtgg gggatagttg ctgagaaaga gtgcggaaga tgtaaaggcg tcggttattc
     2641 aagaatgcca gcaagcgccg catatcgcgc tgtgacgatg ctaatcccaa accttaccca
     2701 acccacctgg tcacgcactg ttaagccgct gtatgacgct ctggttgtgc aatgccacaa
     2761 ggaagagtca atcgcagaca acattttgaa tgcgatcaca cgttagcgcc atgattgcca
     2821 cggatggcaa catattaacg gcataatatt gactttttga ataactttgg ggaaacttga
     2881 caccaataat gggcgttttt tacatgtcat tgatgagtct caataacctg ccgccgagta
     2941 gtttttatgc tctgaattgt atttgtgtag taaacatgct gactgc
//
LOCUS       DAZKHF020000368         2978 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2201S31241
            SAMN43267800-rid25106413.denovo.370, whole genome shotgun sequence.
ACCESSION   DAZKHF020000368 DAZKHF020000000
VERSION     DAZKHF020000368.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267800
            Sequence Read Archive: SRR30307263
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2978)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2978)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 2978)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350581.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350581.2
            Genome Coverage       :: 151x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:21:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,652
            CDSs (total)                      :: 5,557
            Genes (coding)                    :: 5,320
            CDSs (with protein)               :: 5,320
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 237
            CDSs (without protein)            :: 237
            Pseudo Genes (ambiguous residues) :: 0 of 237
            Pseudo Genes (frameshifted)       :: 102 of 237
            Pseudo Genes (incomplete)         :: 130 of 237
            Pseudo Genes (internal stop)      :: 52 of 237
            Pseudo Genes (multiple problems)  :: 41 of 237
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2978
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267800-rid25106413.denovo.370"
                     /serotype="O 26:H11"
                     /isolate="2201S31241"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-01"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            complement(<1..857)
                     /locus_tag="ACHOSC_RS23230"
                     /old_locus_tag="pgaptmp_004646"
     CDS             complement(<1..857)
                     /locus_tag="ACHOSC_RS23230"
                     /old_locus_tag="pgaptmp_004646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308364.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HGU4550427.1"
                     /translation="MIRQKILQQLLEWIECNLEHPISIEDIAQKSGYSRRNIQLLFRN
                     FMHVPLGEYIRKRRLCRAAILVRLTAKSMLDIALSLHFDSQQSFSREFKKLFGCSPRV
                     YRHRDYWDLANIFPSFLIRQQQKTECRLVNFPETPIFGNSFKYDIEVSNKSPDEEVKL
                     RRHHLARCMNNFKTDIYFVSTFEPSTKSVDLLTVETFAGTVCKQTDSEMPKEWTINRG
                     LYASFRYEGEWEHYPEWARNLYLMELPARGLARVNGSDIERFYYNENFVEIDSNNIVC
                     EIFIPVRPV"
     gene            1106..1975
                     /locus_tag="ACHOSC_RS23235"
                     /old_locus_tag="pgaptmp_004647"
     CDS             1106..1975
                     /locus_tag="ACHOSC_RS23235"
                     /old_locus_tag="pgaptmp_004647"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308365.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HGU4550428.1"
                     /translation="MEFSVLSNNLKMPMMGFGVFQVTDKNVCKQSVLNAIRTGYRLID
                     TAAVYGNEDAVGEAVREAISEGLCTRDELFITSKLWVQDMLNQDTAAAGIEASLKKSG
                     LEYFDLYLLHQAMRDYFSAWRALEDAYEEGKLKAIGVSNFYPHVLANFCETVRVKPMV
                     NQVELHPYFAQPEALATMKYYNVQPEAWAPLGGGRHKPFENNLLQSIADAHQKSISQV
                     ILRWNIQRGVVVIPKSTHQQRIEENFAIWDFSLTEKEMAQISSLDLGYVGESVKHFNP
                     EFVRGCLAVKIHD"
     gene            complement(2135..2728)
                     /gene="rclC"
                     /locus_tag="ACHOSC_RS23240"
                     /old_locus_tag="pgaptmp_004648"
     CDS             complement(2135..2728)
                     /gene="rclC"
                     /locus_tag="ACHOSC_RS23240"
                     /old_locus_tag="pgaptmp_004648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414835.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="reactive chlorine species resistance protein
                     RclC"
                     /protein_id="HGU4550429.1"
                     /translation="MEKYLHLLSRGDKIGLTLIRLSIAIVFMWIGLLKFVPYEADSIT
                     PFVANSPLMSFFYEHPEDYKQYLTHEGEYKPEARAWQTANNTYGFSNGLGVVEVIIAL
                     LVLANPVNRWLGLLGGLMAFTTPLVTLSFLITTPEAWVPALGDAHHGFPYLSGAGRLV
                     LKDTLMLAGAVMIMADSAREILKQRSNESSSTLKTEY"
     gene            complement(2740..2976)
                     /gene="rclB"
                     /locus_tag="ACHOSC_RS23245"
                     /old_locus_tag="pgaptmp_004649"
     CDS             complement(2740..2976)
                     /gene="rclB"
                     /locus_tag="ACHOSC_RS23245"
                     /old_locus_tag="pgaptmp_004649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414837.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="reactive chlorine resistance periplasmic protein
                     RclB"
                     /protein_id="HGU4550430.1"
                     /translation="MFKKSVLFATLLSGVMAFSTNADDKIILKHISVSSVSASPTVLE
                     DTIADIARKYNASSWKVTSMRIDNNSTATAVLYK"
BASE COUNT          859 a          610 c          659 g          850 t
ORIGIN      
        1 taaaccggac gaacggggat aaaaatttcg caaacaatgt tattgctatc aatttcgacg
       61 aaattttcat tgtaataaaa ccgctcaata tcactgccgt tcactctggc taaccccctg
      121 gcgggtaatt ccatcagata gaggtttcgc gcccattcag ggtagtgctc ccactcacct
      181 tcatagcgga aagaggcata cagtcctcgg ttaattgtcc actctttcgg catctctgaa
      241 tcggtttgtt tgcataccgt accggcaaaa gtttcaaccg tgagcaaatc gaccgatttt
      301 gttgacggtt caaacgtgga gacgaaatag atatccgtct taaaattatt catacatctg
      361 gctaaatgat gacgtcgcag tttgacttct tcatccggtg atttattcga cacttcaata
      421 tcatatttaa atgaattgcc aaaaataggt gtctcaggaa agttgactaa tctacactcg
      481 gtcttttgct gttgacgtat taaaaaagaa gggaagatat ttgcgagatc ccaataatca
      541 cggtggcggt atacacgggg agagcagccg aataactttt taaattcgcg gctgaatgac
      601 tgctgtgaat caaaatgcaa agagagtgca atatcaagca tagatttcgc ggtgagccgg
      661 acaagaatgg cggcacgaca aagccttcgt ttgcgaatgt attctcccaa aggcacatgc
      721 atgaaatttc ggaacagaag ctggatgttg cgtctgctgt agccagattt ctgtgcgata
      781 tcttcgattg aaatagggtg ctcaagattg cactcaatcc actccaggag ctgctgtaga
      841 atcttctgcc tgatcatcac attctccatt gattcttcgt catatcgcac attttcgtta
      901 tgccctgggc cctaagtgta tcaaaaacag caagggcgaa ggcaaaagca ctggtagcgt
      961 ctgcttcgtt gtccccgaaa atagagaggt ttcagtcctg cattattaat gaattttttg
     1021 cataagtgat atcaaaatcc acgtactaat ttgaggttac gttttaacgt agactcattg
     1081 ttcatgccta atggagggac tgacagtgga attttccgta ttaagtaaca atctgaaaat
     1141 gccgatgatg ggatttggtg tttttcaggt taccgataaa aacgtgtgca aacagtcagt
     1201 gttgaacgct atccgcacag gttatcgact catcgacact gcagcggtat acggtaatga
     1261 agatgccgtc ggtgaagccg ttcgtgaagc tatatctgaa ggtttatgta cccgagatga
     1321 gttatttata acgtctaaat tgtgggtgca ggatatgttg aatcaggata cggcagcagc
     1381 aggtattgaa gcatcattaa aaaaatcggg actagagtac ttcgacctct atttattgca
     1441 ccaggctatg cgcgattatt tcagtgcgtg gcgtgcactc gaagatgcct atgaagaggg
     1501 caaattaaaa gcaattgggg tttccaattt ctatcctcat gttctggcga acttttgtga
     1561 aacggtaaga gttaaaccga tggtcaacca ggtcgagttg catccctatt ttgcccaacc
     1621 agaggcgctg gcaaccatga agtattataa cgtgcagcct gaagcatggg ctccgttagg
     1681 tggtggacga cataaaccct ttgaaaataa tctgcttcag agtattgcag atgcccatca
     1741 aaaatcgatt tctcaagtca ttctgcgttg gaatattcaa cggggagtgg tcgttattcc
     1801 gaaatcgaca catcaacagc gtatcgaaga aaattttgct atctgggatt tctcactgac
     1861 agagaaagaa atggcacaaa ttagttcgct tgatttgggt tatgttgggg agtcggtaaa
     1921 acattttaat cctgaatttg ttcgtggttg tcttgctgta aaaatacatg attgatatta
     1981 atcatatatt ttacctgaga cgacaagaat cttttaacag gggagtgata ttgatcttca
     2041 ctctgtcata tctccggtaa tatggcgtca ggcttcatca tcagaaaggg ggcctgacgc
     2101 ctgaaaaagt gaacaacaga cagtgttcgg attatcaata ttcagttttt aacgttgaac
     2161 tggattcatt actgcgttgt ttaagaattt cccgcgccga atctgccatt atcatgacag
     2221 cacctgccag catcagagta tctttcaata ccaggcgacc agcaccggat aaataaggga
     2281 aaccatgatg agcgtcaccc aatgcgggta cccatgcctc cggggtggtg attaaaaatg
     2341 agagtgttac cagcggcgtg gtaaatgcca tcagtcctcc caataaacct aaccagcgat
     2401 tgacaggatt agccaaaacc agcagagcaa taatcacctc cacgacgcca agaccgttgg
     2461 aaaaaccata ggtattattg gccgtttgcc atgcccttgc ttctggtttg tattcgcctt
     2521 cgtgagtcag atactgttta tagtcttccg ggtgttcata aaagaacgac attagtggac
     2581 tgtttgcgac gaatggtgta atgctgtctg cctcgtaagg gacaaacttt aataacccaa
     2641 tccacataaa aacaattgca atactcagac gaatcaatgt caggccaatt ttatctcccc
     2701 gacttaacag gtgcaggtat ttttccattg tgaacatcct tatttataca atactgctgt
     2761 tgcggttgaa ttattatcaa ttcgcatcga tgtgactttc caggatgaag cattatattt
     2821 tctggctata tcagcaatgg tatcctccag aactgtcggt gatgctgata ctgacgagac
     2881 gctgatatgt ttcagaatta ttttatcatc tgcattggtg gaaaatgcca taacgccaga
     2941 taatagtgtt gcaaataaaa cagatttttt aaacatat
//
LOCUS       DAZKHF020000369         2954 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2201S31241
            SAMN43267800-rid25106413.denovo.371, whole genome shotgun sequence.
ACCESSION   DAZKHF020000369 DAZKHF020000000
VERSION     DAZKHF020000369.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267800
            Sequence Read Archive: SRR30307263
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2954)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2954)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 2954)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350581.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350581.2
            Genome Coverage       :: 151x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:21:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,652
            CDSs (total)                      :: 5,557
            Genes (coding)                    :: 5,320
            CDSs (with protein)               :: 5,320
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 237
            CDSs (without protein)            :: 237
            Pseudo Genes (ambiguous residues) :: 0 of 237
            Pseudo Genes (frameshifted)       :: 102 of 237
            Pseudo Genes (incomplete)         :: 130 of 237
            Pseudo Genes (internal stop)      :: 52 of 237
            Pseudo Genes (multiple problems)  :: 41 of 237
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2954
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267800-rid25106413.denovo.371"
                     /serotype="O 26:H11"
                     /isolate="2201S31241"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-01"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            181..1347
                     /gene="nhaA"
                     /locus_tag="ACHOSC_RS23250"
                     /old_locus_tag="pgaptmp_004650"
     CDS             181..1347
                     /gene="nhaA"
                     /locus_tag="ACHOSC_RS23250"
                     /old_locus_tag="pgaptmp_004650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015385 - sodium:proton antiporter
                     activity [Evidence IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA];
                     GO_process: GO:0006885 - regulation of pH [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter NhaA"
                     /protein_id="HGU4550431.1"
                     /translation="MKHLHRFFSSDASGGIILIIAAILAMIMANSGATSGWYHDFLET
                     PVQLRVGSLEINKNMLLWINDALMAVFFLLVGLEVKRELMQGSLASLRQAAFPVIAAI
                     GGMIVPALLYLAFNYADPITREGWAIPAATDIAFALGVLALLGSRVPLALKIFLMALA
                     IIDDLGAIIIIALFYTNDLSMASLGVAAVAIAVLAVLNLCGVRRTGVYILVGVVLWTA
                     VLKSGVHATLAGVIVGFFIPLKEKHGRSPAKRLEHVLHPWVAYLILPLFAFANAGVSL
                     QGVTLDGLTSILPLGIIAGLLIGKPLGISLFCWLALRLKLAHLPEGTTYQQIMAVGIL
                     CGIGFTMSIFIASLAFGSVDPELINWAKLGILVGSISSAVIGYSWLRVRLRPSV"
     gene            1413..2312
                     /gene="nhaR"
                     /locus_tag="ACHOSC_RS23255"
                     /old_locus_tag="pgaptmp_004651"
     CDS             1413..2312
                     /gene="nhaR"
                     /locus_tag="ACHOSC_RS23255"
                     /old_locus_tag="pgaptmp_004651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_705976.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional activator NhaR"
                     /protein_id="HGU4550432.1"
                     /translation="MSHINYNHLYYFWHVYKEGSVVGAAEALYLTPQTITGQIRALEE
                     RLQGKLFKRKGRGLEPSELGELVYRYADKMFTLSQEMLDIVNYRKESNLLFDVGVADA
                     LSKRLVSSVLNAAVVEGEPIHLRCFESTHEMLLEQLSQHKLDMIISDCPIDSTQQEGL
                     FSVRIGECGVSFWCTNPPPEKPFPACLEERRLLIPGRRSMLGRKLLNWFNSQGLNVEI
                     LGEFDDAALMKAFGAMHNAIFVAPTLYAYDFYADKTVVEIGRVENVMEEYHAIFAERM
                     IQHPAVQRICNTDYSALFSPAAR"
     gene            complement(2351..>2954)
                     /locus_tag="ACHOSC_RS23260"
                     /old_locus_tag="pgaptmp_004652"
     CDS             complement(2351..>2954)
                     /locus_tag="ACHOSC_RS23260"
                     /old_locus_tag="pgaptmp_004652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000871680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="fimbrial family protein"
                     /protein_id="HGU4550433.1"
                     /translation="TIPYSIASVSDSSNTADIFPGTPFPLLFEIEVSSCTGNNTNCNS
                     VRFNYNLNTILQVNLMTCGFEDQSIDTGRFVLSRINNENYQFHHIRFDCIQGEQGELV
                     FEPSDVEYYFEPVTIQESGTSILKNELENSEDGAGQIGFQLSNDGTNEIQYGKSNYYS
                     FQHPHEGSNQIPLFIRPRTYGNNVSSGQIMSRVKIVVMYN"
BASE COUNT          719 a          640 c          739 g          856 t
ORIGIN      
        1 gggcttaggg tcactcgtga gcgcttacag ccgtcaaaaa ctcatctcac cgctgatggc
       61 gcaaattctt caatggatcg taaaaaacga gttattccta cactataatc tgattttaac
      121 gatgatttgt gcggggtaaa atagtaaaaa cgatctattc acctgaaaga gaaataaaaa
      181 gtgaaacatc tgcatcgatt ctttagcagt gatgcctcgg gaggcattat tcttatcatt
      241 gccgctatcc tggcgatgat tatggccaat agcggcgcaa ccagtggatg gtatcacgac
      301 tttctggaga cgccggttca gctccgggtt ggttcactcg aaatcaacaa aaacatgctg
      361 ttatggataa atgacgcgct gatggcggta tttttcctgt tagtcggtct ggaagttaaa
      421 cgtgaactga tgcaaggatc gctagccagc ttacgccagg ccgcatttcc agttatcgcc
      481 gctattggtg ggatgattgt tccggcatta ctctatctgg cctttaacta tgccgatccg
      541 attacccgcg aagggtgggc gatcccggcg gctactgaca ttgcctttgc acttggtgtg
      601 ctggcgctgt tgggaagtcg tgttccgtta gcgctgaaga tctttttgat ggctctggct
      661 attatcgacg atcttggggc catcattatc atcgcattgt tctacactaa tgacttatcg
      721 atggcctctc ttggcgtcgc ggctgtagca attgcggtac tcgcggtatt gaatctgtgt
      781 ggtgtacgcc gcacgggcgt ctatattctg gttggcgtag tgttgtggac tgcggtgttg
      841 aaatcggggg ttcacgcaac cctggcgggg gtaattgtcg gcttctttat tcctttgaaa
      901 gagaagcatg ggcgttctcc agcgaagcga ctggagcatg tgttacaccc gtgggtggcg
      961 tatctgattt tgccgctgtt tgcatttgct aatgctggcg tttcactgca aggtgtcacg
     1021 ctggatggct tgacctccat tctgccattg gggatcatcg ctggcttgct gattggcaaa
     1081 ccgctgggga ttagtctgtt ctgctggttg gcgctgcgtt tgaaactggc gcatctgcct
     1141 gagggaacga cttatcagca aattatggcg gtggggatcc tgtgcggtat cggttttact
     1201 atgtctatct ttattgccag cctggccttt ggtagcgtag atccagaact gattaactgg
     1261 gcgaaactcg gtatcctggt cggttctatc tcttcggcgg taattggata cagctggtta
     1321 cgcgttcgtt tgcgtccatc agtttgacag gacggtttac cggggagcca taaacggctc
     1381 ccttttcatt gttatcaggg agagaaatga gcatgtctca tatcaattac aaccacttgt
     1441 attacttctg gcatgtctat aaagaaggtt ccgtggttgg cgcagcggag gcgctttatt
     1501 taactccaca aaccattacc ggacagattc gagcgctgga agagcgcctg caaggcaaat
     1561 tatttaaacg caagggacgt ggtctcgaac ccagcgagct gggagaactg gtctatcgct
     1621 atgccgataa aatgttcacc ttaagccagg aaatgctgga tattgtgaac tatcgcaaag
     1681 aatccaattt attgtttgac gttggtgtgg ctgatgcact ttccaaacgc ctggtcagta
     1741 gcgtacttaa cgccgcagtg gtagaaggcg agcccattca tcttcgctgc ttcgaatcca
     1801 cccacgaaat gctgctggag caattaagtc agcataaact ggatatgatc atttctgact
     1861 gtccgataga ctctacgcag caggaaggcc tgttctccgt gagaattggc gaatgtggcg
     1921 tgagtttctg gtgtacaaat ccaccaccag aaaaaccgtt cccggcttgt ctggaagaac
     1981 ggcgactttt gattcctggg cgacgttcaa tgttagggcg caaattgctt aactggttta
     2041 actcccaggg attaaacgta gaaatcctcg gcgagtttga tgatgccgcg ttgatgaaag
     2101 cttttggtgc gatgcacaat gcaatcttcg ttgccccaac gctttatgca tatgactttt
     2161 atgccgataa aactgtcgta gaaattggtc gcgtcgagaa tgtgatggaa gagtaccatg
     2221 ctatttttgc tgagcggatg attcagcacc cggcggtaca gcgaatctgc aatacggatt
     2281 attctgcgct ttttagtcca gcggcgcgtt aatcggcacc tccccccaaa gttaaggtgg
     2341 gggagataga ttagttgtac ataaccacga ttttgactcg gctcattatt tgcccgcttg
     2401 agacattatt tccatatgta cgcgggcgaa taaatagagg aatctgatta cttccttcat
     2461 ggggatgctg aaaagagtag taattgcttt taccatactg gatttcattc gtaccatcat
     2521 tggagagttg aaagcctatt tgacccgcac cgtcctcact gttttctaac tcatttttta
     2581 atatgctggt tcctgactcc tgtatcgtca ctggttcaaa gtaatattca acatcactgg
     2641 gttcaaaaac taattcacct tgctcgccct ggatgcagtc aaatcttata tgatgaaact
     2701 gataattttc attattgata cgagacagta caaatcgtcc agtgtcgatg gattgatctt
     2761 caaaaccaca ggtcattaaa tttacctgta aaatagtatt taaattataa ttaaagcgga
     2821 cgctattaca attagtatta ttccctgtac atgaactcac ttcaatttcg aacaacaaag
     2881 gaaaaggagt tccaggaaaa atgtctgcgg tgttagaact atctgataca gatgcgatag
     2941 aatatggaat ggtg
//
LOCUS       DAZKHF020000370         2898 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2201S31241
            SAMN43267800-rid25106413.denovo.372, whole genome shotgun sequence.
ACCESSION   DAZKHF020000370 DAZKHF020000000
VERSION     DAZKHF020000370.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267800
            Sequence Read Archive: SRR30307263
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2898)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2898)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 2898)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350581.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350581.2
            Genome Coverage       :: 151x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:21:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,652
            CDSs (total)                      :: 5,557
            Genes (coding)                    :: 5,320
            CDSs (with protein)               :: 5,320
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 237
            CDSs (without protein)            :: 237
            Pseudo Genes (ambiguous residues) :: 0 of 237
            Pseudo Genes (frameshifted)       :: 102 of 237
            Pseudo Genes (incomplete)         :: 130 of 237
            Pseudo Genes (internal stop)      :: 52 of 237
            Pseudo Genes (multiple problems)  :: 41 of 237
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2898
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267800-rid25106413.denovo.372"
                     /serotype="O 26:H11"
                     /isolate="2201S31241"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-01"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            complement(221..1699)
                     /locus_tag="ACHOSC_RS23265"
                     /old_locus_tag="pgaptmp_004653"
     CDS             complement(221..1699)
                     /locus_tag="ACHOSC_RS23265"
                     /old_locus_tag="pgaptmp_004653"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0019200 - carbohydrate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FGGY-family carbohydrate kinase"
                     /protein_id="HGU4550434.1"
                     /translation="MSKKYIIGIDGGSQSTKVVMYDLEGSVVCEGKGLLQPMHTPYAD
                     TAEHPDDDLWASLCFAGHDLMSQFAGNKEDIVGIGLGSIRCCRALLKADGTPAAPLIS
                     WQDARVTRPYEHTNPDVAYVTSFSGYLTHRLTGEFKDNIANYFGQWPVDYKSWAWSED
                     AAVMDKFNIPRHMLFDVQMPGTVLGHITPQAALATHFPAGLPVVCTTSDKPVEALGAG
                     LLDDETAVISLGTYIALMMNGKALPKDPVAYWPIMSSIPQTLLYEGYGIRKGMWTVSW
                     LRDMLGESLIQDARAQDLSPEDLLNKKASSVPPGCNGLMTVLDWLTNPWEPYKRGIMI
                     GFDSSMDYAWIYRSILESVALTLKNNYDNMCNEMNHFAKHVIITGGGSNSDLFMQIFA
                     DVFNLPARRNAINGCASLGAAINTAVGLGLYPDYATAVDKMVRVKDIFIPIESNAKRY
                     DAMNKGIFKDLTKHTDVILKKSYEVMHGELGNVDSIQSWSNA"
     gene            complement(1726..>2898)
                     /locus_tag="ACHOSC_RS23270"
                     /old_locus_tag="pgaptmp_004654"
     CDS             complement(1726..>2898)
                     /locus_tag="ACHOSC_RS23270"
                     /old_locus_tag="pgaptmp_004654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate/nitrite transporter"
                     /protein_id="HGU4550435.1"
                     /translation="MAKFMGFSNTEIGLIMSTFGIAAIILYAPSGVIADKFSHRKMIT
                     SAMIITGLLGLLMATYPPLWVMLCIQVAFAITTILMLWSVSIKAASLLGDHSEQGKIM
                     GWMEGLRGVGVMSLAVFTMWVFSRFAPDDSASLKTVIIIYSVVYILLGILCWFFVSDN
                     NNLRSANNEEKQSFQLSDILAVLRISTTWYCSMVIFGVFTIYAILSYSTNYLTEMYGM
                     SLVAASYMGIVINKIFRALCGPLGGIITTYSKVKSPTRVIQILSVLGLLALTALLVTN
                     SNPQSVAMGIGLILLLGFTCYASRGLYWACPGEVRTPSYIMGTTVGICSVIGFLPDVF
                     VYPIIGHWQDTLPAAEAYRNMWLMGMAALAMVIVFTFLLFQKIRTADSAPAMASSK"
BASE COUNT          762 a          759 c          654 g          723 t
ORIGIN      
        1 tattgtcttt aattctaaag acatcgagtg tagttaatat tccttgtaaa aacagggata
       61 aaccgaacta gttaaagttt ttattatgtt ccctgcatca gcggggataa accggacttg
      121 ttatcatttt ttaagttgtc gaacttatgt tccccgcgtc agcggggata accatttagg
      181 tatgttcagg ccgggcagtt ttccgcccgg cctacctcat tcacgcattc gaccagctct
      241 ggatcgaatc cacattcccc aattccccat gcatcacttc atacgatttt ttcaggatca
      301 catcagtatg tttggttagg tctttgaaaa tgcctttatt catcgcgtcg tagcgtttgg
      361 cattgctctc aatcggtata aagatatctt tcacgcgaac catcttatca acagccgttg
      421 cgtaatccgg gtatagcccc agacctaccg ctgtattaat cgctgctccc agacttgcac
      481 aaccgttaat ggcgttacgg cgtgccggaa ggttgaacac gtcggcaaaa atttgcataa
      541 acagatcgct gttcgaaccg ccgccagtaa tgatcacatg cttcgcaaag tgattcattt
      601 cattacacat attgtcgtaa ttgttcttca gcgttagcgc cacgctttcc aatatcgaac
      661 gatatatcca tgcgtaatcc atgctggaat caaagccgat cataatcccg cgtttgtacg
      721 gttcccacgg attggtcagc cagtccagca ccgtcatcag cccattacag ccaggtggca
      781 cactagaggc tttcttgttg agtaaatctt ccggtgaaag atcctgcgcc ctggcatcct
      841 gaattaacga ctcgcctaac atgtcacgca gccagctcac cgtccacata cctttgcgaa
      901 taccgtaacc ttcatacagc aatgtttgcg gaatagaaga cataatcggc cagtacgcca
      961 ccggatcttt cggcagtgct ttgccgttca tcatcaatgc gatgtaagtg ccaagagaaa
     1021 ttaccgcagt ttcatcatcc agtaatccgg cccccagagc ttctaccggt ttatcactgg
     1081 tggtacaaac aaccggcagt cctgccggga aatgtgtcgc cagtgcggct tgtggtgtga
     1141 tatgtccgag gacggtgcca ggcatttgca catcaaacag catatgacgg gggatattaa
     1201 acttatccat taccgcagca tcttcgctcc atgcccagct cttataatcc accggccact
     1261 gaccaaaata gttggcgata ttgtctttaa actcgccggt taagcgatgc gtcagataac
     1321 ccgaaaaaga ggtgacatac gccacgtcag gattcgtatg ttcgtaaggg cgtgtaacgc
     1381 gtgcatcctg ccagctaatc aacggcgcag caggcgtgcc atcggctttc aataacgcac
     1441 ggcagcaacg gatggagccc aggccgatgc caacaatatc ttctttattc ccggcaaact
     1501 ggctcatcaa atcgtgacct gcaaaacata atgatgccca taaatcgtcg tcaggatgtt
     1561 ctgcagtatc ggcataaggc gtgtgcatcg gctgtaataa gcctttacct tcgcaaacca
     1621 cactaccttc cagatcgtac atcaccactt ttgtgctctg acttccgcca tcaatcccta
     1681 tgatgtattt tttcgacatg atttttctcc tgtaattttc ctggcttact tgctgctagc
     1741 cattgcgggg gcgctatcag cagtacgaat tttttggaac agcaaaaagg taaagacaat
     1801 caccatggca agcgccgcca ttcccatcag ccacatattg cggtaggctt ctgctgcggg
     1861 cagagtgtct tgccagtggc cgataatcgg gtaaacgaag acatccggca ggaatccaat
     1921 caccgaacaa atacctaccg tggtgcccat aatgtaagac ggtgttctca cttcgccagg
     1981 gcaggcccag tacagcccgc gtgaggcgta acaggtgaat cccagcagta aaatcaggcc
     2041 aatccccatc gcgaccgatt gcgggttaga gttcgtgacg agcagggcag ttaacgccag
     2101 caggccgagt acggaaagga tttggatcac gcgggtaggg gatttcactt tactgtaggt
     2161 ggtgataatg ccgccaagtg ggccgcacag cgcgcggaag attttgttga tcacaatccc
     2221 catgtagctc gctgccacca gcgacatgcc gtacatttcg gtcagatagt tggtggagta
     2281 actcagaatg gcgtagattg tgaagacacc aaaaatcacc atgctgcaat accaggtggt
     2341 gctgatacgc aaaacggcaa ggatgtcgct aagctggaat gactgttttt cttcgttatt
     2401 ggcactgcgc aggttgttgt tatcgctaac aaaaaaccag cacagaatcc ccaacaagat
     2461 gtaaaccaca ctgtagatga taatgactgt tttcaggctg gcgctgtcat ccggtgcaaa
     2521 gcgagaaaag acccacatgg taaacaccgc cagcgacatt acaccgacgc cgcgcagccc
     2581 ttccatccag cccataattt tcccttgctc actatgatcg cccagcaacg aggcggcttt
     2641 aatcgacacc gaccacagca ttaaaatcgt cgttatcgca aaggcgacct gaatacagag
     2701 cattacccac agcggtggat acgttgccat taacagaccc agtaatccgg taatgatcat
     2761 cgcggaagta atcattttgc ggtgtgaaaa tttatcggca ataacgccgc tgggggcata
     2821 aagaataata gccgcaatac caaaggtact cattattaaa cctatctcgg tattgctgaa
     2881 tcccataaat ttcgccat
//
LOCUS       DAZKHF020000371         2897 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2201S31241
            SAMN43267800-rid25106413.denovo.373, whole genome shotgun sequence.
ACCESSION   DAZKHF020000371 DAZKHF020000000
VERSION     DAZKHF020000371.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267800
            Sequence Read Archive: SRR30307263
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2897)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2897)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 2897)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350581.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350581.2
            Genome Coverage       :: 151x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:21:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,652
            CDSs (total)                      :: 5,557
            Genes (coding)                    :: 5,320
            CDSs (with protein)               :: 5,320
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 237
            CDSs (without protein)            :: 237
            Pseudo Genes (ambiguous residues) :: 0 of 237
            Pseudo Genes (frameshifted)       :: 102 of 237
            Pseudo Genes (incomplete)         :: 130 of 237
            Pseudo Genes (internal stop)      :: 52 of 237
            Pseudo Genes (multiple problems)  :: 41 of 237
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2897
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267800-rid25106413.denovo.373"
                     /serotype="O 26:H11"
                     /isolate="2201S31241"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-01"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            <1..1383
                     /gene="tssI"
                     /locus_tag="ACHOSC_RS23275"
                     /old_locus_tag="pgaptmp_004655"
     CDS             <1..1383
                     /gene="tssI"
                     /locus_tag="ACHOSC_RS23275"
                     /old_locus_tag="pgaptmp_004655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system tip protein TssI/VgrG"
                     /protein_id="HGU4550436.1"
                     /translation="QEGQHQDYQRTQYEVYDYPGRFKGAHGQNFARWQMDGWRNNAET
                     ARGMSRSPEIWPGRRIVLTGHPQANLNREWQVVASELHGEQPQAVPGRQGAGTALENH
                     FAVIPADRTWRPQPLLKPLVDGPQSAVVTGPAGEEIFCDEHGRVRVKFNWDRYNPADQ
                     DSSCWIRVAQAWAGTGFGHLAIPRVGQEVIVDFLNGDPDQPIIMGRTYHHENRTPGSL
                     PGTKTQMTIRSKTYKGSGFNELKFDDATGKEQVYIHAQKNMNTEVLNNRTTDVINNHA
                     ETIGNNQMIAVTNNQIQTVGVNQIETVGSNQIINVGSVQVETIGLVRALTVGVAYQTT
                     VGGIMNTSVALMQSSQIGLHKSLRVGLGYDVKVGNNVTFTVGKTKKDDTGQTAIYSAG
                     EHLELCCGKARLVLTKDGQIFLNGTKIHLQGKEQVNGDSLLINWNCAASKSPPKPPDE
                     KQDTPDMREY"
     gene            1451..>2897
                     /gene="rhsD"
                     /locus_tag="ACHOSC_RS23280"
                     /old_locus_tag="pgaptmp_004656"
     CDS             1451..>2897
                     /gene="rhsD"
                     /locus_tag="ACHOSC_RS23280"
                     /old_locus_tag="pgaptmp_004656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhs element protein RhsD"
                     /protein_id="HGU4550437.1"
                     /translation="MSGKPAARQGDMTQYGGPIVQGSAGVRIGAPTGVACSVCPGGMT
                     SGNPVNPLLGAKVLPGETDLALPGPLPFILSRTYSSYRTKTPAPVGVFGPGWKAPSDT
                     RLQLRDDGLILNDNGGRSIHFEPLLPGEAVYSRSESMWLVRGGKAAQPDGHTLARLWG
                     ALPPDIRLSPHLYLATNSAQGPWWILGWSERVPGAEDVLPAPLPPYRELTGLADRFGR
                     TLTYRREAAGDLTGEITGVTDGAGREFRLVLTTQAQRAEEARTSSLSSSDSSRPLSAS
                     AFPDTLPGTEYGPDRGIRLSAVWLMHDPAYPESLPAAPLVRYTYTEAGELLAVYDRSN
                     TQVRAFTYDAQHPGRMVAHRYAGRPEMRYRYDDAGRVVEQLNPAGLSYRYQYEQDRIT
                     VTDSLNRREVLHTEGGAGLKRVVKKELADGSVTHSGYDAAGRLTAQTDAAGRRTEYGL
                     NVVSGDITDITTPDGRETKFYYNDGNQLTAVV"
BASE COUNT          648 a          781 c          973 g          495 t
ORIGIN      
        1 caggaaggcc agcaccagga ttaccagcgc acacagtatg aagtgtatga ctaccccgga
       61 cgtttcaagg gggcccacgg gcagaacttt gcccgctggc agatggacgg ctggcgaaac
      121 aatgcagaaa ccgcgcgggg aatgagtcgc tcgccggaga tatggccggg acgacgaatt
      181 gtgctgacgg ggcatccgca ggcgaacctg aaccgggaat ggcaggtggt ggcaagtgaa
      241 ctgcacggcg aacagccaca ggcggtgcca ggacggcagg gagcggggac ggcgctggag
      301 aaccattttg cggtgatccc ggcagacaga acatggcgac cacagccgtt gctgaaaccg
      361 ctggtcgacg gcccgcagag cgctgtcgtg acaggaccgg caggcgagga aatcttctgc
      421 gacgaacatg gtcgcgtgcg ggtgaagttc aactgggacc gttataaccc ggcagaccaa
      481 gacagttcgt gctggatccg tgtggcacag gcgtgggcag gcaccggttt tggccacctg
      541 gcgataccgc gtgtgggtca ggaggtgatt gtggacttcc tcaacggcga cccggaccag
      601 ccgattatta tggggcgtac ctaccaccat gaaaaccgca cacccggcag cctgccgggg
      661 acgaagacgc agatgacgat ccgctcgaaa acctataagg gcagcgggtt taatgaactg
      721 aagtttgacg atgcgacagg gaaagaacag gtctacatcc acgcgcagaa gaacatgaac
      781 accgaggtgc tgaataaccg caccacggat gtgataaaca accatgctga gaccattggc
      841 aacaatcaga tgattgcggt taccaacaat cagatacaga cggtgggcgt taaccagata
      901 gagacggtgg gcagtaacca gatcattaac gtgggttctg ttcaggtgga aacgattgga
      961 cttgttcgtg cgctgaccgt gggcgtggcg taccagacga cggtaggtgg cattatgaac
     1021 acttcggtgg ctctgatgca gtcctcgcag atcggtttgc ataaatcgtt gagggtcggg
     1081 ctgggttatg acgtcaaagt cggaaataac gttaccttca ccgttggtaa aacgaaaaag
     1141 gatgataccg ggcagaccgc gatttactcc gccggtgagc atctggagct ctgctgtggt
     1201 aaggcaaggc tggtgctgac gaaggacgga caaatttttc tcaacggcac aaaaattcat
     1261 ttgcagggta aggagcaggt taatggtgac tcactgttga ttaactggaa ctgtgcagcc
     1321 tcgaaatccc caccgaagcc ccctgatgaa aagcaggata cgccggatat gagagagtac
     1381 tgatcccgta cctgttcgga attcagtaac aaaccagcat taaaaataaa cagaagaagg
     1441 aatttgaatc atgagcggaa aaccagcggc gcgtcaggga gatatgactc agtatggcgg
     1501 tcccattgtc cagggttcgg caggtgtaag aattggcgcg cccaccggcg tggcgtgctc
     1561 ggtgtgtccg ggcgggatga cttcgggcaa cccggtaaat ccgctgctgg gggcgaaggt
     1621 gctgcccggc gagacggacc ttgcgctgcc cggcccgctg ccgttcattc tctcccgcac
     1681 ctacagcagc taccggacga agacgcctgc accggtgggc gttttcggcc ccggctggaa
     1741 agcgccttct gatacccgct tacagctacg tgatgacgga ctgatactca acgacaacgg
     1801 cgggcggagc attcactttg agccgctgct gccgggggag gcggtgtaca gccgcagtga
     1861 gtcaatgtgg ctggtgcgcg gtggtaaggc agcacagccg gacggccata cgctggcgcg
     1921 gctgtggggg gcgctgccgc cggatatccg gttaagcccg catctttacc tggcgaccaa
     1981 cagcgcacag gggccgtggt ggatactcgg ctggtcagag cgggtgccgg gtgctgagga
     2041 cgtgctgcca gcgccgctgc cgccgtaccg ggagctgacg gggctggcgg accgcttcgg
     2101 gcggacgctg acgtaccggc gtgaggccgc cggtgacctg accggggaaa tcaccggcgt
     2161 gacggacggt gccgggcggg agttccgtct ggtgctgacc acgcaggcgc agcgtgcgga
     2221 agaggcccgc acctcttcgc tatcttcttc tgacagttcc cgccctctct cagcctcagc
     2281 gttccccgac acactgcccg gtaccgaata cggccccgac aggggtatcc gcctttcggc
     2341 ggtgtggctg atgcacgacc cggcataccc ggagagcctg cccgctgcgc cactggtgcg
     2401 gtacacatat acggaagccg gtgaactgct ggcggtatat gaccgcagca atacgcaggt
     2461 gcgcgctttc acgtatgacg cgcagcaccc gggccggatg gtggcgcacc gttatgcagg
     2521 caggccggag atgcgctacc gctacgacga tgcggggcgg gtggtggagc aacttaaccc
     2581 ggcaggcctg agttaccgct accagtatga gcaggatcgc atcaccgtca cggacagcct
     2641 gaaccggcgt gaggtgctgc atacagaagg cggggccggg ctgaagcggg tggtgaaaaa
     2701 agaactggcg gacggcagcg tcacgcacag cgggtatgac gcggcaggaa ggctcacggc
     2761 gcagacggac gcggcgggac ggcggacaga gtacggtctg aatgtggtgt caggcgatat
     2821 cacggacatc accacgccgg acgggcggga gacgaaattt tactataacg acgggaacca
     2881 gctgacggcg gtggtgt
//
LOCUS       DAZKHF020000372         2883 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2201S31241
            SAMN43267800-rid25106413.denovo.374, whole genome shotgun sequence.
ACCESSION   DAZKHF020000372 DAZKHF020000000
VERSION     DAZKHF020000372.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267800
            Sequence Read Archive: SRR30307263
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2883)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2883)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 2883)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350581.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350581.2
            Genome Coverage       :: 151x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:21:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,652
            CDSs (total)                      :: 5,557
            Genes (coding)                    :: 5,320
            CDSs (with protein)               :: 5,320
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 237
            CDSs (without protein)            :: 237
            Pseudo Genes (ambiguous residues) :: 0 of 237
            Pseudo Genes (frameshifted)       :: 102 of 237
            Pseudo Genes (incomplete)         :: 130 of 237
            Pseudo Genes (internal stop)      :: 52 of 237
            Pseudo Genes (multiple problems)  :: 41 of 237
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2883
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267800-rid25106413.denovo.374"
                     /serotype="O 26:H11"
                     /isolate="2201S31241"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-01"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            complement(49..651)
                     /locus_tag="ACHOSC_RS23285"
                     /old_locus_tag="pgaptmp_004657"
     CDS             complement(49..651)
                     /locus_tag="ACHOSC_RS23285"
                     /old_locus_tag="pgaptmp_004657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001590672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4550438.1"
                     /translation="MKPQYQTRYELLHESYQKWLTGFTRHAVSWGVCHPNIYYFHNLT
                     PGWVSFNGEKPEIAIVPQSLHRLIYGPDKRTTPPLDDDLIVNLCTSEHLLVHHPMLEG
                     ILLSECERLRQRSLANKLISLFRQFGGTELRLKLVWLCWLDLMTGNCLDDWTENLKRK
                     SEKELEEWIINRQKQSAALTDLMDQYVLLAYRTTVDDKRT"
     gene            complement(738..944)
                     /locus_tag="ACHOSC_RS23290"
                     /old_locus_tag="pgaptmp_004658"
     CDS             complement(738..944)
                     /locus_tag="ACHOSC_RS23290"
                     /old_locus_tag="pgaptmp_004658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001564245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HGU4550439.1"
                     /translation="MITPVSLMDDQMVDMAFITQLTGLTDKWFYKLIKDGDFPAPIKM
                     GRSSRWLKSEVEAWLQARIAQSRP"
     gene            1696..1845
                     /locus_tag="ACHOSC_RS23295"
                     /old_locus_tag="pgaptmp_004659"
     CDS             1696..1845
                     /locus_tag="ACHOSC_RS23295"
                     /old_locus_tag="pgaptmp_004659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015704109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hha/YmoA family nucleoid-associated regulatory
                     protein"
                     /protein_id="HGU4550440.1"
                     /translation="MEKVISHTRYKLTPAEMEAFNSAVDHRLAELTMNKLYDRVPASV
                     WKYVI"
     gene            2657..>2883
                     /locus_tag="ACHOSC_RS23300"
                     /old_locus_tag="pgaptmp_004660"
     CDS             2657..>2883
                     /locus_tag="ACHOSC_RS23300"
                     /old_locus_tag="pgaptmp_004660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000221568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inovirus-type Gp2 protein"
                     /protein_id="HGU4550441.1"
                     /translation="MTYKYNPFWQQRIRETVRHALDVHPRLTALRVDLRLPDVPAATD
                     AAVISRFINALKARIDAYQKRKHREGKRVHPT"
BASE COUNT          749 a          687 c          765 g          682 t
ORIGIN      
        1 cagccctggc accctgcccg gataccagct tccctgaaac aactggcatc aggtgcgctt
       61 atcgtcaacc gttgtgcggt atgccaggag cacgtactga tccatcagat ccgtcagtgc
      121 tgcgctctgc ttctgacggt tgatgatcca ttcctccagc tctttttctg atttccgttt
      181 cagattctcc gtccagtcgt caaggcagtt tccggtcatt aaatcaagcc agcaaagcca
      241 gaccagtttg aggcgcaact ccgtgccacc aaactgacgg aagaggctga tcagtttatt
      301 tgccagcgaa cgctgcctca gacgttcaca ttcagacagg agaatgcctt ccagcatcgg
      361 atgatgaacc agcagatgtt cactggtgca taaattcact atcagatcat catccagggg
      421 cggcgtggtc cgtttgtccg gaccataaat cagccggtgc agactctggg gtacgatggc
      481 aatctccggt ttttcaccat tgaatgacac ccacccgggc gtcagattat ggaagtagta
      541 gatattcgga tgacacacgc cccaggatac ggcgtgccgg gtgaagccgg tcagccattt
      601 ctggtagctt tcgtggagca gctcgtaacg ggtctgatac tgtggtttca ttttcatatt
      661 cctcacgggg gataacaggc agcagaacgc cgccggcagg ccggagactg cgtctgctgc
      721 agatgaacgg ttaagaatta cggacgggac tgtgcaatac gcgcctgcag ccaggcttcc
      781 acttcgcttt tcagccatcg ggagctgcgg cccattttga tgggcgcagg aaagtcacca
      841 tccttgatga gtttgtaaaa ccacttatcg gtcaggccgg tcagttgagt gataaatgcc
      901 atgtcgacca tctggtcatc catcagtgaa actggggtga tcatgagaat gttcctccgg
      961 atgttgttaa accagccggt gtggtcatga aaacaacgca ccaccttcac cggcataaca
     1021 accggggaga aacgtaaaaa ataatcggga atgtgaccgc caggagataa acggggaggt
     1081 tacggtggct gagccgtacc ggtatgccgg gtggttgtta cgaaacgatg cttccgcacg
     1141 cgcgcgcgat aaagaaggga tgattttcgt gaggaagagg ggaaaactgg cgttctgata
     1201 attgcagagt tatttacggg gcggttacga tggggcagac cgtgtccggt gggcaggcga
     1261 caggtatcac cgatgggata agactgtctg attggtatta taaatatata agagagatca
     1321 aacacatgag atcaaataca tgagttcata tatgtgaaat aaacacggtg agtgtgatca
     1381 gtgggcatga tgagaacgca gtagcaacag actgcgctat ccctgatagc tggcgtataa
     1441 cctgcgtata taaaacgtac cagcaacgca cgtgaacaga gtgcattaag catccgtcgt
     1501 tgacaccgac ctggtgagtg acagtctgag agaccggtta agtggtggta cgggagtaat
     1561 gagtttgggg agggtaccag aatgtcagtc tggtacggta tgctgacagt atcacgttaa
     1621 ttgcagcata aagtgatgag aggctaatgg aagttaaaac cagagcagtt ccgtcgttgt
     1681 tcatcccggg agacactgga aaaagtgatt tcccacacgc gttataagct tactccagcg
     1741 gagatggaag ccttcaactc tgcggtcgat caccgactgg cagaactgac aatgaacaag
     1801 ctttacgatc gtgttcccgc ttcagtctgg aaatatgtca tctgagcgtt cggtaatcac
     1861 aactgcatcg cgctaggtgt tatccgcttt gtgattctta ttggctcttc tcaggttatt
     1921 aaaagctgaa tatgttcctg ttgtcgggaa ccaggataat accgggagcg ttaagcggta
     1981 tagcgacaac aaagaattcg aaatcaaaag ggaatggtgc cggaggcgtg ggcggtaata
     2041 ggctgatttt caggtattac cggcttacaa cgtgtggttg atattgaggg gctgtgagtt
     2101 tcgcgtattt gtcagaaacc cagatgagtt ttcctgtatg ggacgaaata aggcagcggc
     2161 aatacctgga aaatttaata tggtatgaag gagaacctgt cggttacgct taatatacac
     2221 ggtacactgt cagctcaggg gatacatgaa ccgttatctc accagctaca cagtatacac
     2281 acactgacgc tgacagtagt cagcaggacc acaataatca gcgccagcag tatggtaacg
     2341 atacggtctg tattccggta gtagccgaag gggtcgacag aaccacaata cgggcaacac
     2401 actgtattat tagtaatcgc gtttccacag tccctgcact tcttttgcgt atcaccataa
     2461 ctccctcctc acagccttac ccgtaaccgg ttttttacat taaaaaacaa cgctgaaaat
     2521 tcaaccgtca gtccggagac gaccgttcgg gttatcacag agtgcctgag acagtgtcct
     2581 gccggaggtc acttcaggga ctctgcgcat tttttacgac gtggctattc cgtatgaacc
     2641 atacggagat ttaaccatga cctacaaata caaccccttc tggcagcaac gtattcgtga
     2701 gacggtgcgg cacgcactgg atgttcatcc ccgtctgacg gcattgcggg ttgatctgcg
     2761 tctcccggat gtaccggcag caacggacgc agctgtgata tcccgcttca tcaatgccct
     2821 gaaagcccga atcgacgctt accagaaacg taagcatcgg gaaggtaaac gcgtgcatcc
     2881 cac
//
LOCUS       DAZKHF020000373         2860 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2201S31241
            SAMN43267800-rid25106413.denovo.375, whole genome shotgun sequence.
ACCESSION   DAZKHF020000373 DAZKHF020000000
VERSION     DAZKHF020000373.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267800
            Sequence Read Archive: SRR30307263
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2860)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2860)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 2860)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350581.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350581.2
            Genome Coverage       :: 151x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:21:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,652
            CDSs (total)                      :: 5,557
            Genes (coding)                    :: 5,320
            CDSs (with protein)               :: 5,320
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 237
            CDSs (without protein)            :: 237
            Pseudo Genes (ambiguous residues) :: 0 of 237
            Pseudo Genes (frameshifted)       :: 102 of 237
            Pseudo Genes (incomplete)         :: 130 of 237
            Pseudo Genes (internal stop)      :: 52 of 237
            Pseudo Genes (multiple problems)  :: 41 of 237
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2860
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267800-rid25106413.denovo.375"
                     /serotype="O 26:H11"
                     /isolate="2201S31241"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-01"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            <1..912
                     /locus_tag="ACHOSC_RS23305"
                     /old_locus_tag="pgaptmp_004661"
     CDS             <1..912
                     /locus_tag="ACHOSC_RS23305"
                     /old_locus_tag="pgaptmp_004661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001666873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="zeta toxin family protein"
                     /protein_id="HGU4550442.1"
                     /translation="EKDALLNGASQCIAFLSNIIDNCSVSSHQDSGDALKRLVSYRDY
                     LYSTPAELVDFTQGKILLQQVRTQYQHEFNNTTHSENKASFDSIWQRLTNHEVTPQQH
                     PIGFVLGGQPGAGKSSLIELAKRETKDNIMIINGDDFRFLHPDFNYIYQNYGDDFVTH
                     TAKFSGETVERAIERAIVSKLNIVVEGTFRNAATPLQTLKKLKDAGYQTEVMIKTTSA
                     ALSWESTNERYSKDKEAGNIARKVDKNHHDIVTGLLAENARKVFASNLSDKFAVYSRE
                     KMIFSSQAATNDDIATLIQNEISGNTQ"
     gene            complement(948..1739)
                     /locus_tag="ACHOSC_RS23310"
                     /old_locus_tag="pgaptmp_004662"
     CDS             complement(948..1739)
                     /locus_tag="ACHOSC_RS23310"
                     /old_locus_tag="pgaptmp_004662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001130549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase domain-containing
                     protein"
                     /protein_id="HGU4550443.1"
                     /translation="MPLNGVSAAMRERVSQQLKEIERRYDVKVLYACESGSRGWGFAS
                     PDSDYDVRFLYVHPLEWYLRVESPRDVIELPIDDELDVSGWEWRKALGLLKGANPTLI
                     EWLDSPVVYQQDEETITAFKAMVPMWFSPLRARWHYYSMAQKNFPGYLQGDEVRLKKY
                     FYVLRPLLAVRWVEAGKGVPPMRFAELLAGSELDAPLRAEIDELLERKQRAGEAEYGP
                     RRPLLHAFIRAELARGEIPPLLPDSREGDVKELDSLMYQTVMRRA"
     gene            complement(1745..>2860)
                     /locus_tag="ACHOSC_RS23315"
                     /old_locus_tag="pgaptmp_004663"
     CDS             complement(1745..>2860)
                     /locus_tag="ACHOSC_RS23315"
                     /old_locus_tag="pgaptmp_004663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000592028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="slipin family protein"
                     /protein_id="HGU4550444.1"
                     /translation="ISVRKDQLALLSRNGDYYKVLHAGEHLLPWLNTPEVLLITLDGS
                     EVPDVLADYLRRFQPDWVEKYCLVADLSEIEAGALYMDGILQEILPPSTRRLYWRVED
                     DLTLVRMNTQQVQVQTEVMNAVLQPRRKGAVKGRDAILTVQVPAWHVGVLKIDGETQA
                     LLPPGLTAYWKINHLVDAEVVDTRLQVLEVSGQEILTKDKVNLRINLAANWRYSDVLL
                     AFSQLTKPIDHLYRELQFALREAVGTRTLDELLEDKQVIDDVVSEQVKSRMLPFGMEI
                     ASLGVKDIVLPGDMKNILAQLVEAEKSAQANVIRRREETAATRSLLNTAKVMENNPVA
                     LRLKELETLERVAERIDNISVFGGLDQVLHGLVNIKG"
BASE COUNT          760 a          767 c          604 g          729 t
ORIGIN      
        1 gaaaaagatg cacttttaaa cggagccagt cagtgcatcg cgtttttatc aaatatcatt
       61 gataattgct cagtttcgtc gcatcaggat tcaggcgatg ctctcaagcg attagtcagc
      121 tatcgcgatt atctttattc tacccccgct gaacttgttg attttacaca aggtaagatt
      181 ctccttcagc aggtaagaac tcaatatcag catgaattca acaatactac gcattcagaa
      241 aataaagcat cttttgattc tatttggcag cgtctgacaa atcatgaagt cacaccgcaa
      301 cagcatccca ttggttttgt tttaggtggg caacccggag caggtaaatc atctctgatt
      361 gaattagcca aaagagagac aaaagataat ataatgatta ttaacggtga tgattttcga
      421 tttctacacc ctgattttaa ttatatctac caaaattatg gggatgattt tgttacccat
      481 acagcgaaat tttccggcga aacggtcgaa cgtgcaattg agagagcgat agtcagtaaa
      541 ctcaacattg ttgtcgaagg aacatttcgt aatgccgcga cgccgttaca aacattaaaa
      601 aaactcaaag atgcaggcta ccaaacagaa gtcatgataa aaacaacctc agcggcatta
      661 agctgggaaa gcaccaatga acggtatagt aaggacaaag aggcgggaaa tattgcccga
      721 aaagtggata aaaaccacca tgatatcgtc accggtttgc tggcagaaaa tgcccgcaag
      781 gtgtttgctt cgaacctgtc agataagttt gcggtgtatt cgcgtgagaa gatgatattt
      841 agttcacaag cggcaacgaa tgacgatatt gcaacgctta tacagaatga aatcagcggg
      901 aatacacaat aataataaga gctgatggcg tgaaataacg ccgggcttta tgcccggcgc
      961 attaccgtct ggtacattaa gctatccagt tccttaacgt ccccttctct gctatccggt
     1021 aacagcggtg gaatttcccc acgcgccagt tccgcacgaa taaacgcatg taacaatgga
     1081 cggcgcggac catattccgc ttctcctgcg cgctgtttac gttccagtaa ttcgtctatt
     1141 tctgcacgta acggcgcatc aagttcgctt cccgccagta actcagcaaa gcgcattggc
     1201 ggtacgcctt tacccgcttc gacccagcgc acggccagca gcggacgcag cacgtaaaag
     1261 tattttttca gtcgaacttc gtcgccctgc aaatatccgg ggaagttttt ctgcgccatc
     1321 gaatagtaat gccagcgcgc acgcagcgga gaaaaccaca ttgggaccat tgccttaaac
     1381 gccgttatcg tttcttcatc ttgttgataa accaccggag aatccagcca ttctatcagc
     1441 gtcgggttcg cgcctttcaa taatcccagc gccttacgcc actcccaacc ggaaacatct
     1501 aactcgtcgt cgatgggcag ctcaataaca tcgcgcggtg actctactcg cagataccat
     1561 tccagcgggt gaacatacaa aaaccgtaca tcgtagtcgc tgtcgggcga ggcaaatccc
     1621 cagccgcggc tccctgattc acaggcgtac agtactttca catcataccg ccgttcaatt
     1681 tcttttaact gctgcgatac cctttctcgc atcgcagcac tgactccatt taatggcatg
     1741 ttactcaccc tttgatatta accaacccat gcagaacctg atccagaccg ccaaatacag
     1801 agatgttatc gatccgttca gccactctct ccagcgtttc cagttctttt aagcgtaacg
     1861 ctaccgggtt gttttccatg actttagcgg tattgagtaa cgaacgcgtc gctgccgttt
     1921 cttcacgtcg gcgaatcaca ttggcctgcg cggatttctc cgcctcaacc agctgcgcaa
     1981 ggatattttt catatcgcca ggcaggacga tatctttcac ccctaacgag gcaatctcca
     2041 taccaaatgg caacatgcgc gatttcacct gttcgctgac aacgtcatca atgacctgtt
     2101 tatcttccag caattcatcc aacgtacgcg tacctaccgc ttcacgcagg gcaaattgta
     2161 attcccgata caaatgatca atcggcttcg ttaactggct aaatgccaat aacacgtcgc
     2221 tgtagcgcca gttagcggcg aggttaatcc gcagattgac cttatctttc gttaagattt
     2281 cttgcccgct gacttccagt acctgcaagc gggtgtcaac cacttcagcg tcaaccagat
     2341 ggttaatttt ccagtatgcg gtcagccccg gcggtaacag cgcctgcgtt tcaccatcga
     2401 ttttcagcac gcctacatgc caggccggaa cctgcaccgt tagtattgca tcgcggcctt
     2461 taactgcccc tttgcgccgt ggttgcaata ccgcattcat cacctccgtt tgtacctgga
     2521 cttgctgcgt attcatccgc accagtgtca aatcatcctc tacacgccag tacaaacggc
     2581 gcgttgatgg cggcaggatc tcctgcaaaa taccgtccat atacaacgcc ccggcttcaa
     2641 tctccgacaa gtctgccacc aggcaatatt tctccaccca atcaggctga aagcggcgca
     2701 aataatccgc cagcacgtcg ggaacttcgc tgccatcgag cgtaatcagc aagacttctg
     2761 gcgtatttaa ccagggtaaa agatgctcgc ctgcatgtag cactttgtag taatcgccgt
     2821 ttctggagag taatgccagc tgatcttttc ttacgcttat
//
LOCUS       DAZKHF020000374         2837 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2201S31241
            SAMN43267800-rid25106413.denovo.376, whole genome shotgun sequence.
ACCESSION   DAZKHF020000374 DAZKHF020000000
VERSION     DAZKHF020000374.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267800
            Sequence Read Archive: SRR30307263
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2837)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2837)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 2837)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350581.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350581.2
            Genome Coverage       :: 151x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:21:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,652
            CDSs (total)                      :: 5,557
            Genes (coding)                    :: 5,320
            CDSs (with protein)               :: 5,320
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 237
            CDSs (without protein)            :: 237
            Pseudo Genes (ambiguous residues) :: 0 of 237
            Pseudo Genes (frameshifted)       :: 102 of 237
            Pseudo Genes (incomplete)         :: 130 of 237
            Pseudo Genes (internal stop)      :: 52 of 237
            Pseudo Genes (multiple problems)  :: 41 of 237
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2837
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267800-rid25106413.denovo.376"
                     /serotype="O 26:H11"
                     /isolate="2201S31241"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-01"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            181..1412
                     /locus_tag="ACHOSC_RS23320"
                     /old_locus_tag="pgaptmp_004664"
                     /pseudo
     CDS             181..1412
                     /locus_tag="ACHOSC_RS23320"
                     /old_locus_tag="pgaptmp_004664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309413.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoadenosine phosphosulfate reductase"
     gene            1397..2035
                     /locus_tag="ACHOSC_RS23325"
                     /old_locus_tag="pgaptmp_004665"
     CDS             1397..2035
                     /locus_tag="ACHOSC_RS23325"
                     /old_locus_tag="pgaptmp_004665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParB N-terminal domain-containing protein"
                     /protein_id="HGU4550445.1"
                     /translation="MGDSVTPEILGNMIRQYFSQERPEEEAIQALNHLRRVLHEVSPF
                     AQEPVDCVLWVKADEVVANDYNPNVMAPGEKKLLKQSLEKDGFTQPVVVSEEKNHYLV
                     VDGFHRQVLGREAGTGKRLKGWLPVVCINPERKGQASRIAATIRHNRARGKHQITSMS
                     DIVRDLSRLGWTNERIGTELGMDQDEVLRLKQISGLTELFQEEDFSPAWTVR"
     gene            2114..2383
                     /locus_tag="ACHOSC_RS23330"
                     /old_locus_tag="pgaptmp_004666"
     CDS             2114..2383
                     /locus_tag="ACHOSC_RS23330"
                     /old_locus_tag="pgaptmp_004666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PerC family transcriptional regulator"
                     /protein_id="HGU4550446.1"
                     /translation="MVAQKLEAAGCWRRASDRWLFVMGNVECTEAQREWLLLRRNYCL
                     AQISSPPLPETLDISEVAKAADATLRRMGIATPSGEVFRKGTPVC"
     gene            2404..>2837
                     /locus_tag="ACHOSC_RS23335"
                     /old_locus_tag="pgaptmp_004667"
     CDS             2404..>2837
                     /locus_tag="ACHOSC_RS23335"
                     /old_locus_tag="pgaptmp_004667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4550447.1"
                     /translation="MMKQGMANSISMTWQLLCDEHGFTREHYNQLKKFSPETLREIIA
                     EIASCHPSTSVLLRNKWLTPPEDILEQITREYERRIQNCPPFRSEKEAESWLNELSFA
                     VIAPLYRVAPEQTEQVESFVLQLIAEQERVWELILTNDGYSW"
BASE COUNT          727 a          604 c          798 g          708 t
ORIGIN      
        1 catggatttt ccagatgtgt taccagtaag aagcaggtgg ataaaggatg tgataacaaa
       61 tccaacaacc agattaatat ctgaagagtt ttattgttat tatttatccc caaagcggtg
      121 agttattaat gaagtttgtc tgaatgcata aacagaatta ttcgacataa agccaggaaa
      181 atgagtgata cgcttttaac cgaaaaaatc cttaccggag aaaatgtttt gcgtgccgca
      241 atcgcccgca ttgagtggat ttttgaaaca tttccgtcgg tatgcctgtc tttttcaggt
      301 ggaaaggatt ccactgtgct tttccatctt gtggcagagg tggcccgcag gagaaaacgt
      361 catttctctg ttctgttcat tgactgggag gcccagtatc ggtgcactat tgaacatatt
      421 cagaagatgc gggaaatgta ccatgatgtg acggaaacct tttactgggt ggcacttccc
      481 tgaccacggt aaacggcgtc tcgcagtttc agccggagtg gatatgctgg gagccaggag
      541 tgacctgggt tcgccagcca ccggaagagg ccattacaga catgacgtat tttccctttt
      601 accggtatgc catgacgttt gaagaatttg ttccggcatt ttcttcctgg tttgccggta
      661 accggtgtgg agtggcaata ctgactggtg tccgtgctga tgaatcgctc aatcgcttta
      721 tgggactggt atctcagcgc aaactgagat atgccgatga taagccctgg accacggcat
      781 cgcctgaagg gttttattac accatgtatc cgttgtatga ctggaaggcc cgcgatatct
      841 ggatatataa cgccagaacc tgtgccattt acaatcctct gtatgacctg atgtaccgtg
      901 ccggcgtgcc gttacgcaac atgcgtgtct gtgaaccttt tggtcctgaa cagcgtaagg
      961 gactgtggct ttaccatgtt ctggagccgg aaacctgggc caggatgtgt gaacgggtgt
     1021 caggggctgc cagcggggcg ctttatgcca atgagagtgg tgcctatttt gccctgtgta
     1081 agcgtatcac gaagccgcct catcatacct ggcgcagcta tgcgatgttc ctgctggatg
     1141 tgatgccgga aagaacagca gaacattacc gtaataaaat tgctgtctac ctgcgttggt
     1201 atcagacgcg gggcttcccg gatgacattc cggatgaaca ggagaatgac ctggggagcc
     1261 gggatattcc gtcctggcga cgtatctgta agactctcat aaagaatgat ttctggtgcc
     1321 ggaccctctc ctttagccct aacaaacccc ggcactatga acgttacctg cagcgtatga
     1381 aggaaaggag gaaggaatgg gggattctgt gacaccggag atacttggca acatgattcg
     1441 tcagtacttc agccaggaac gaccagaaga agaggccata caggccctta atcacctgcg
     1501 cagggtgctt catgaggtca gtccttttgc acaggagcct gtggactgtg tgctgtgggt
     1561 aaaagcagat gaggtggtgg ccaatgatta caatcctaat gtcatggcac cgggtgagaa
     1621 gaaactgctg aagcagtcac tggagaaaga tggtttcaca caaccggtgg tagtgtctga
     1681 ggaaaagaac cattacttgg tggtggatgg ttttcatcgc caggtactgg gcagggaagc
     1741 aggtacggga aaacgtctga agggctggct gccggtggtc tgtattaatc cggagagaaa
     1801 gggacaggcc tcacgtattg ctgcgaccat ccgacataac cgggccaggg gcaaacatca
     1861 gataacctca atgtctgata ttgtcaggga cctgtcgcgc ctgggctgga cgaatgaacg
     1921 cattggtacg gagctgggta tggaccagga tgaagttctc agattgaaac agattagcgg
     1981 gctgacggag ctgtttcagg aagaggattt cagcccggca tggacggttc gctgagaccg
     2041 tcagatatgt atattatttc agtgagttaa tgttttgagc aggaaaaaga gtaccccgga
     2101 agttgtggaa gacatggttg cgcagaaact ggaggctgct ggttgctggc gcagagcttc
     2161 tgaccgctgg ctttttgtta tgggaaatgt tgaatgtaca gaagcacaac gggagtggtt
     2221 gttgttgcgc cggaattatt gtctggcgca gatatcgtcc ccgccgctac ctgaaacgct
     2281 ggatatcagc gaggtggcga aagctgcaga tgccacgctg aggcgaatgg ggattgccac
     2341 tccgtccggg gaggtattca gaaaaggtac accggtctgt tgatattcag gtaacaggta
     2401 ttaatgatga agcaaggaat ggcaaacagt atatcaatga catggcaact tttatgcgat
     2461 gaacatggtt ttacccggga gcactacaat cagctgaaga agttcagccc ggagacatta
     2521 cgtgaaatta ttgctgaaat agccagctgt catccatcaa ccagtgtatt gctcaggaat
     2581 aagtggctga caccgccgga ggatattctg gagcagatta cccgtgaata cgaacgaaga
     2641 atacaaaact gtccaccgtt caggagcgag aaggaagcgg aatcctggtt aaatgaacta
     2701 tcttttgctg tgattgctcc gctataccgt gttgcccccg aacaaacaga acaggttgag
     2761 tcctttgttc tgcagcttat tgccgagcag gagcgggttt gggagcttat tttaaccaat
     2821 gatggttatt catggca
//
LOCUS       DAZKHF020000375         2816 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2201S31241
            SAMN43267800-rid25106413.denovo.377, whole genome shotgun sequence.
ACCESSION   DAZKHF020000375 DAZKHF020000000
VERSION     DAZKHF020000375.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267800
            Sequence Read Archive: SRR30307263
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2816)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2816)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 2816)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350581.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350581.2
            Genome Coverage       :: 151x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:21:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,652
            CDSs (total)                      :: 5,557
            Genes (coding)                    :: 5,320
            CDSs (with protein)               :: 5,320
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 237
            CDSs (without protein)            :: 237
            Pseudo Genes (ambiguous residues) :: 0 of 237
            Pseudo Genes (frameshifted)       :: 102 of 237
            Pseudo Genes (incomplete)         :: 130 of 237
            Pseudo Genes (internal stop)      :: 52 of 237
            Pseudo Genes (multiple problems)  :: 41 of 237
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2816
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267800-rid25106413.denovo.377"
                     /serotype="O 26:H11"
                     /isolate="2201S31241"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-01"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            <1..466
                     /gene="agaB"
                     /locus_tag="ACHOSC_RS23340"
                     /old_locus_tag="pgaptmp_004668"
     CDS             <1..466
                     /gene="agaB"
                     /locus_tag="ACHOSC_RS23340"
                     /old_locus_tag="pgaptmp_004668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="PTS galactosamine transporter subunit IIB"
                     /protein_id="HGU4550448.1"
                     /translation="NILLTRIDNRLVHGQVGVTWTSTIGANLLVVVDDVVANDDIQQK
                     LMGITAETYGFGIRFFTIEKTINVIGKAAPHQKIFLICRTPQTVRKLVEGGIDLKDIN
                     VGNMHFSEGKKQISSKVYVDDQDLADLRFIKQRGVNVFIQDVPGDQKEQIPD"
     gene            505..1308
                     /gene="agaC"
                     /locus_tag="ACHOSC_RS23345"
                     /old_locus_tag="pgaptmp_004669"
     CDS             505..1308
                     /gene="agaC"
                     /locus_tag="ACHOSC_RS23345"
                     /old_locus_tag="pgaptmp_004669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS galactosamine transporter subunit IIC"
                     /protein_id="HGU4550449.1"
                     /translation="MHEITLLQGLSLAALVFVLGIDFWLEALFLFRPIIVCTLTGAIL
                     GDIQTGLITGGLTELAFAGLTPAGGVQPPNPIMAGLMTTVIAWSTGVDAKTAIGLGLP
                     FSLLMQYVILFFYSAFSLFMTKADKCAKEADTAAFSRLNWTTMLIVASAYAVIAFLCT
                     YLAQGAMQALVKAMPAWLTHGFEVAGGILPAVGFGLLLRVMFKAQYIPYLIAGFLFVC
                     YIQVSNLLPVAVLGAGFAVYEFFNAKSRQQAQPQPVASKNEEEDYSNGI"
     gene            1298..2089
                     /gene="agaD"
                     /locus_tag="ACHOSC_RS23350"
                     /old_locus_tag="pgaptmp_004670"
     CDS             1298..2089
                     /gene="agaD"
                     /locus_tag="ACHOSC_RS23350"
                     /old_locus_tag="pgaptmp_004670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708938.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS galactosamine transporter subunit IID"
                     /protein_id="HGU4550450.1"
                     /translation="MGSEISKKDITRLGFRSSLLQASFNYERMQAGGFTWAMLPILKK
                     IYKDDKPGLSAAMKDNLEFINTHPNLVGFLMGLLISMEEKGENRDTIKGLKVALFGPI
                     AGIGDAIFWFTLLPIMAGICSSFASQGNLLGPILFFAVYLLIFFLRVGWTHVGYSVGV
                     KAIDKVRENSQMIARSATILGITVIGGLIASYVHINVVTSFAIDSTHSVALQQDFFDK
                     VFPNILPMAYTLLMYYFLRVKKAHPVLLIGVTFVLSIVCSAFGIL"
     gene            2090..>2816
                     /locus_tag="ACHOSC_RS23355"
                     /old_locus_tag="pgaptmp_004671"
     CDS             2090..>2816
                     /locus_tag="ACHOSC_RS23355"
                     /old_locus_tag="pgaptmp_004671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactosamine-6-phosphate isomerase"
                     /protein_id="HGU4550451.1"
                     /translation="MERGTASGGASLLKEFHPVQTLQQVENYTALSERASEYLLAVIR
                     SKPDAVICLATGATPLLTYHYLVEKIHQQQVDISQLTFVKLDEWVDLPLTMPGTCETF
                     LQQHIVQLLGLREDQLISFRSEEINETECERVTNLIARKGGLDLCVLGLGKNGHLGLN
                     EPGESLQPACHISQLDARTQQHEMLKTTGRPVTRGITLGLKDILNAREVLLLVTGEGK
                     QDATERFLTAKVSTAIPASFLWLH"
BASE COUNT          679 a          668 c          718 g          751 t
ORIGIN      
        1 aaatattctc ttaacccgta ttgataaccg tctggttcac ggtcaggttg gcgtgacctg
       61 gacatccacc atcggtgcga atctgctggt agtcgtggat gatgttgtcg ctaacgatga
      121 tattcaacag aaattaatgg gtattaccgc agaaacctac ggctttggca ttcgtttctt
      181 tactatcgaa aaaaccatta acgtcatcgg caaagctgca ccgcatcaga agatcttcct
      241 gatttgccgt acgccacaaa cggtacgtaa attggtagaa ggtggcattg acctgaaaga
      301 tatcaacgtc ggcaatatgc atttctcgga agggaaaaag caaatcagca gtaaagttta
      361 tgtcgatgac caggatctcg cggacttacg ttttattaaa caacgtggcg tgaatgtttt
      421 cattcaggac gtccctggcg atcaaaaaga acaaatccct gactaaatct aaaatcgcct
      481 taatattggt ttgaggtaat aaaaatgcat gaaataaccc tacttcaggg attatccctg
      541 gcggcgttag ttttcgttct ggggattgat ttttggctgg aagccttatt tttattccgc
      601 ccgataatcg tttgtaccct aactggcgct attctcggtg atattcagac tggcttaatt
      661 accggtggtc tgacagagtt ggctttcgcc ggattaaccc ctgcaggtgg tgttcagccg
      721 cccaacccga ttatggcggg tctgatgacc accgtcattg catggtctac gggcgttgat
      781 gccaaaacag caattggtct tggcctgccg tttagtttgt taatgcagta cgtcattctg
      841 ttcttctatt ccgctttctc attatttatg accaaagccg ataaatgcgc gaaagaggcg
      901 gatacggcag cgttttcccg gcttaactgg acaacgatgc tcatcgtcgc ttcagcgtat
      961 gcggtgattg ctttcctctg tacttacctg gcacaagggg cgatgcaggc gctggtgaaa
     1021 gcgatgcccg cctggttgac ccacggcttt gaagtggctg gcggtattct gcctgccgtt
     1081 ggttttggct tgctgctgcg cgtaatgttc aaagcgcaat atatccctta cctgatcgcc
     1141 ggtttcctgt ttgtttgcta catccaggtc agcaacctgt tgccggttgc cgtactgggc
     1201 gcaggctttg cggtgtatga gtttttcaat gcgaaatccc ggcagcaagc gcaaccgcag
     1261 cccgttgcca gtaaaaatga agaagaggac tacagcaatg ggatctgaaa tcagtaaaaa
     1321 agatatcacc cgtctgggct ttcgttcgtc gctgctgcaa gcgagcttta actacgaaag
     1381 gatgcaggcg ggcggtttta cctgggcgat gttgccgatc ctgaaaaaga tttataagga
     1441 cgacaaaccg ggcttaagcg cggcgatgaa agataacctc gaatttatta atacccaccc
     1501 gaatctggtc ggattcctga tggggttatt aatttcgatg gaagaaaaag gagaaaaccg
     1561 cgacaccatt aaaggcctca aagtggcact gtttggccca atcgccggga ttggcgatgc
     1621 gattttctgg tttactttgt tgccgattat ggcgggaatt tgctcatcat ttgccagcca
     1681 gggaaacctg ctggggccga ttctattttt cgccgtttac ctgcttatct ttttcctgcg
     1741 cgtcggctgg acccacgtcg gttattcagt cggcgtgaag gcgatcgata aagtgcgaga
     1801 gaactcgcag atgattgctc gctcggcaac catcctcggg atcacggtaa tcggcgggct
     1861 gatcgcttcg tatgtgcata ttaacgtggt gacatcgttt gccatcgaca gtacccacag
     1921 cgtcgcgctg caacaggatt tcttcgataa agtcttcccg aacattttac cgatggccta
     1981 caccctgctg atgtattact tcctgcgggt gaaaaaagcg catccggtgc tgttaatcgg
     2041 cgtgactttt gtgctctcta ttgtttgttc cgcattcggc attttgtaaa tggaacgagg
     2101 cactgcttct ggcggtgcct ctttacttaa ggaatttcat cctgtgcaaa cccttcagca
     2161 agttgaaaac tatacggcgt taagtgaacg agccagcgaa tatttattgg ctgtgatccg
     2221 tagcaaaccg gatgccgtga tttgcctggc gaccggagcc acgccattac tgacgtatca
     2281 ttatctggta gaaaaaatcc accagcagca ggttgatatc agccagctca ccttcgtgaa
     2341 gctcgacgaa tgggtggatc tgccattaac gatgcctggc acctgcgaaa ctttcctaca
     2401 acagcatatc gtgcagctgc tggggctgcg tgaagaccag ctcatcagct ttcgctccga
     2461 agagataaat gagacagagt gcgaacgggt aacgaacctg attgcgcgca aaggcggtct
     2521 ggatttatgc gttctaggat tggggaaaaa cggtcatctt gggctgaacg aaccgggaga
     2581 aagcctacaa ccggcctgcc atatcagtca acttgatgcc agaacacagc aacatgagat
     2641 gttaaaaacc acgggtcgcc ccgtgactcg tgggatcacc ttaggcctga aggatattct
     2701 caatgcccgc gaggttttgt tattggtgac tggcgaagga aagcaggatg cgacagaacg
     2761 ttttctcacg gctaaagtct ccaccgctat cccggcttcg tttttatggc tgcaca
//
LOCUS       DAZKHF020000376         2810 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2201S31241
            SAMN43267800-rid25106413.denovo.378, whole genome shotgun sequence.
ACCESSION   DAZKHF020000376 DAZKHF020000000
VERSION     DAZKHF020000376.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267800
            Sequence Read Archive: SRR30307263
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2810)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2810)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 2810)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350581.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350581.2
            Genome Coverage       :: 151x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:21:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,652
            CDSs (total)                      :: 5,557
            Genes (coding)                    :: 5,320
            CDSs (with protein)               :: 5,320
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 237
            CDSs (without protein)            :: 237
            Pseudo Genes (ambiguous residues) :: 0 of 237
            Pseudo Genes (frameshifted)       :: 102 of 237
            Pseudo Genes (incomplete)         :: 130 of 237
            Pseudo Genes (internal stop)      :: 52 of 237
            Pseudo Genes (multiple problems)  :: 41 of 237
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2810
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267800-rid25106413.denovo.378"
                     /serotype="O 26:H11"
                     /isolate="2201S31241"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-01"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            complement(114..190)
                     /locus_tag="ACHOSC_RS23360"
                     /old_locus_tag="pgaptmp_004672"
     tRNA            complement(114..190)
                     /locus_tag="ACHOSC_RS23360"
                     /old_locus_tag="pgaptmp_004672"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(154..156),aa:Asp,seq:gtc)
     gene            complement(323..1054)
                     /gene="dnaQ"
                     /locus_tag="ACHOSC_RS23365"
                     /old_locus_tag="pgaptmp_004673"
     CDS             complement(323..1054)
                     /gene="dnaQ"
                     /locus_tag="ACHOSC_RS23365"
                     /old_locus_tag="pgaptmp_004673"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706156.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit epsilon"
                     /protein_id="HGU4550452.1"
                     /translation="MSTAITRQIVLDTETTGMNQIGAHYEGHKIIEIGAVEVVNRRLT
                     GNNFHVYLKPDRLVDPEAFGVHGIADEFLLDKPTFAEVADEFMDYIRGAELVIHNAAF
                     DIGFMDYEFSLLKRDIPKTNTFCKVTDSLAVARKMFPGKRNSLDALCARYEIDNSKRT
                     LHGALLDAQILAEVYLAMTGGQTSMAFAMEGETQQQQGEATIQRIVRQASKLRVVFAT
                     DEELAAHEARLDLVEKKGGSCLWRA"
     gene            1119..1586
                     /gene="rnhA"
                     /locus_tag="ACHOSC_RS23370"
                     /old_locus_tag="pgaptmp_004674"
     CDS             1119..1586
                     /gene="rnhA"
                     /locus_tag="ACHOSC_RS23370"
                     /old_locus_tag="pgaptmp_004674"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004523 - RNA-DNA hybrid ribonuclease
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HI"
                     /protein_id="HGU4550453.1"
                     /translation="MLKQVEIFTDGSCLGNPGPGGYGAILRYRGREKTFSAGYTRTTN
                     NRMELMAAIVALEALKEHCEVILSTDSQYVRQGITQWIHNWKKRGWKTADKKPVKNVD
                     LWQRLDAALGQHQIKWEWVKGHAGHPENERCDELARAAAMNPTLEDTGYQVEV"
     gene            complement(1583..2305)
                     /locus_tag="ACHOSC_RS23375"
                     /old_locus_tag="pgaptmp_004675"
     CDS             complement(1583..2305)
                     /locus_tag="ACHOSC_RS23375"
                     /old_locus_tag="pgaptmp_004675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308236.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HGU4550454.1"
                     /translation="MKPARVPQTVVAPDRWGDLPWGELYRKALERQLNPWFTKMYGFH
                     LLKIGNLSAEINCEACAVSHQVNVSAQGMPVQVQADPLHLPFADKSVDVCLLAHTLPW
                     CTDPHRLLRETDRVLIDDGWLVISGFNPISLMGLRKLVPVLRKTSPYNSRMFTLMRQL
                     DWLSLLNFEVLHASRFHVLPWNKHGGKLLNAHIPALGCLQLIVARKRTIPLTLNPMKQ
                     SKNKPRIRQAVGATRQYRKPQA"
     gene            2339..>2810
                     /gene="gloB"
                     /locus_tag="ACHOSC_RS23380"
                     /old_locus_tag="pgaptmp_004676"
     CDS             2339..>2810
                     /gene="gloB"
                     /locus_tag="ACHOSC_RS23380"
                     /old_locus_tag="pgaptmp_004676"
                     /EC_number="3.1.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004416 - hydroxyacylglutathione hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0019243 - methylglyoxal catabolic process
                     to D-lactate via S-lactoyl-glutathione [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyacylglutathione hydrolase"
                     /protein_id="HGU4550455.1"
                     /translation="MNLNSIPAFDDNYIWVLNDEAGRCLIVDPGDAEPVLNAIAANNW
                     QPEAIFLTHHHHDHVGGVKELVEKFPQIVVYGPQETQDKGTTQVVKDGETAFVLGHEF
                     SVIATPGHTLGHICYFSKPYLFCGDTLFSGGCGRLFEGTASQMYQSLNKLSALPD"
BASE COUNT          755 a          694 c          664 g          697 t
ORIGIN      
        1 acatttacag cataacaaaa ccatagaccg aagaaatagc gggatttaaa tgcaaaaaaa
       61 accgcaaaat taaaaatctt gcggttctct gaactcattt tcatgagtga atttggcgga
      121 acggacggga ctcgaacccg cgaccccctg cgtgacaggc aggtattcta accgactgaa
      181 ctaccgctcc gcgttgtgtt ccgttgggaa cgaggcgaat attacggatt gcctcaccct
      241 tcgtcaacgg tttttctcat cttttgaatc gtttgctgca aaaatcgccc aagccgctat
      301 ttttagcgcc ttttacaggt atttatgccc gccagaggca gcttccgccc ttcttctcca
      361 ccagatcaag acgggcttca tgagctgcaa gctcttcatc tgtcgcaaaa acaacgcgta
      421 acttacttgc ctgacgtaca atgcgctgaa ttgttgcttc accttgttgc tgctgtgtct
      481 ctccttccat cgcaaaagcc atcgacgttt gaccaccggt catcgccaga taaacttccg
      541 caaggatctg tgcatcgagt aatgccccgt gtagcgttcg tttactgtta tctatttcgt
      601 agcgggcaca taacgcatcg aggctgttgc gcttaccagg aaacattttc ctcgccaccg
      661 caaggctatc ggtgacctta cagaaggtat tggtcttcgg aatatcgcgc ttaagcaacg
      721 aaaactcgta gtccataaag ccgatatcga acgctgcgtt atggatcacc aactccgcgc
      781 cgcggatata gtccatgaac tcatcggcta cttcggcaaa cgtgggctta tcgagcaaaa
      841 attcatcggc aataccatgt acgccaaagg cttccggatc caccagccga tcgggtttga
      901 gataaacatg gaagttattg cccgtcaggc gacggttcac cacttcaacg gcaccaatct
      961 caatgatctt gtggccttca tagtgcgcac caatctggtt cataccggtg gtttcggtat
     1021 cgagaacgat ctggcgtgta attgcagtgc tcatagcggt catttatgtc agacttgtcg
     1081 ttttacagtt cgattcaatt acaggaagtc taccagagat gcttaaacag gtagaaattt
     1141 tcaccgatgg ttcgtgtctg ggtaatcctg gacctggggg ttacggcgct attttacgct
     1201 atcgcggacg cgagaaaacc tttagcgcag gctacacccg caccaccaac aaccgtatgg
     1261 agttgatggc cgctattgtc gcgctggagg cgttaaaaga acattgcgaa gtcattttga
     1321 gtaccgacag ccagtatgtc cgccaaggta tcacccagtg gatccataac tggaaaaaac
     1381 gtggctggaa aacagcagac aaaaaaccag taaaaaatgt cgatctctgg caacgtcttg
     1441 atgctgcatt ggggcagcat caaatcaaat gggaatgggt taaaggccat gccggacacc
     1501 cggaaaacga acgctgtgat gaactggccc gtgctgcggc aatgaatccc acactggaag
     1561 atacaggcta tcaagtcgaa gtttaagcct gtggtttacg atattgccgg gtggctccaa
     1621 ccgcctggcg aattcgtggc ttgtttttac tctgtttcat cggatttagc gttaaaggaa
     1681 tagtccgttt ccgggcaaca ataagttgta agcaaccaag cgcaggaata tgtgcattca
     1741 atagttttcc accgtgtttg ttccacggga gaacgtggaa acggctggcg tgcagcactt
     1801 caaaattcaa caaagagagc cagtccagtt gccgcatcag agtaaacatc cggctgttat
     1861 agggcgaggt ttttcgcaat accggcacaa gtttgcgtaa tcccattaaa ctaatgggat
     1921 tgaagccact aatcaccagc cagccatcat caatcaatac ccgatcggtt tcacgcaata
     1981 aacgatgcgg atcggtgcac cacggcaatg tatgtgccag tagacaaaca tcaacggatt
     2041 tatcggcaaa aggaagatga agtggatccg cctgcacctg gacgggcatt ccttgcgcag
     2101 aaacattcac ttgatgagaa accgcgcacg cttcgcaatt gatttctgcg cttaaattgc
     2161 caatcttaag cagatgaaaa ccatacattt tagtgaacca cgggttgagc tggcgctcca
     2221 gcgccttgcg ataaagctct ccccagggca aatcgcccca gcgatcagga gccacgacag
     2281 tttgagggac tcttgccggt ttcatcacaa ccttccgttt cacactaaga ggtaatctat
     2341 gaatcttaac agtattcccg cctttgatga caattacatc tgggttttga atgatgaagc
     2401 aggtcgctgc ctgattgtcg atcccggaga cgcagagcca gtattaaacg ccattgccgc
     2461 caataactgg caaccggagg ccatatttct cacccaccat catcacgatc acgttggcgg
     2521 cgtaaaagaa ctggtagaaa agtttccgca aattgtggtg tatggtccac aagagacaca
     2581 agataaggga acaacacagg tagtcaaaga tggcgaaact gccttcgttt tggggcatga
     2641 atttagtgta attgccacac cgggtcacac tttaggacat atctgttact tcagtaaacc
     2701 ttatctattt tgcggcgaca cgctgttttc tggtgggtgt ggtaggttgt ttgaagggac
     2761 agcatcacaa atgtatcaat cacttaataa gttaagtgcg ttacctgacg
//
LOCUS       DAZKHF020000377         2801 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2201S31241
            SAMN43267800-rid25106413.denovo.379, whole genome shotgun sequence.
ACCESSION   DAZKHF020000377 DAZKHF020000000
VERSION     DAZKHF020000377.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267800
            Sequence Read Archive: SRR30307263
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2801)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2801)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 2801)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350581.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350581.2
            Genome Coverage       :: 151x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:21:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,652
            CDSs (total)                      :: 5,557
            Genes (coding)                    :: 5,320
            CDSs (with protein)               :: 5,320
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 237
            CDSs (without protein)            :: 237
            Pseudo Genes (ambiguous residues) :: 0 of 237
            Pseudo Genes (frameshifted)       :: 102 of 237
            Pseudo Genes (incomplete)         :: 130 of 237
            Pseudo Genes (internal stop)      :: 52 of 237
            Pseudo Genes (multiple problems)  :: 41 of 237
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2801
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267800-rid25106413.denovo.379"
                     /serotype="O 26:H11"
                     /isolate="2201S31241"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-01"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            <1..135
                     /locus_tag="ACHOSC_RS23385"
                     /old_locus_tag="pgaptmp_004677"
     CDS             <1..135
                     /locus_tag="ACHOSC_RS23385"
                     /old_locus_tag="pgaptmp_004677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PerC family transcriptional regulator"
                     /protein_id="HGU4550456.1"
                     /translation="CRKAQRPPEPPDNYGDLKKAVDRTYAEMGIDGAGDEIWRNYQDS
                     "
     gene            92..220
                     /locus_tag="ACHOSC_RS23390"
                     /old_locus_tag="pgaptmp_004678"
     CDS             92..220
                     /locus_tag="ACHOSC_RS23390"
                     /old_locus_tag="pgaptmp_004678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001301569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trigger factor"
                     /protein_id="HGU4550457.1"
                     /translation="MVLVMKYGAITRTANHTAGEIRLFLHQLKRYGALPGFRHGLA"
     gene            261..485
                     /locus_tag="ACHOSC_RS23395"
                     /old_locus_tag="pgaptmp_004679"
     CDS             261..485
                     /locus_tag="ACHOSC_RS23395"
                     /old_locus_tag="pgaptmp_004679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309616.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4550458.1"
                     /translation="MPMKFDEILKQRDKYHADNMETMSINDYRAFLETGALIEKDQHG
                     FVRCALSGEMLAVNPEQIDALIEFLKGIRD"
     gene            777..1934
                     /locus_tag="ACHOSC_RS23400"
                     /old_locus_tag="pgaptmp_004680"
     CDS             777..1934
                     /locus_tag="ACHOSC_RS23400"
                     /old_locus_tag="pgaptmp_004680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage major capsid protein"
                     /protein_id="HGU4550459.1"
                     /translation="MPRIIELRQQKTAIKNQMRDMLENAEKENRSLNDAEGAKFDELR
                     AKAESLDKDISRLEAIADEERSKPGKSSQTTDPAELRNYILTGETRALSTGVPADGGY
                     TVIPELNTEIMRMLADESTMRRICTVKKISSNEFKQLVSAGGATVNHGEEGKAREQTS
                     PPQINEVSIKLYPVYAYPRTTQEIVDFSDVDILSWLTGEIGDTFTETEESDLVVGDGD
                     KKAKGFLSVPRAEKNDKERDFGTLQVIKPSESLAWTSADPLIDLKFALRKKYRKNAVW
                     VVNSTTAAKLQKVKNANGDYIWRDRLQAGDPDTLLGLPVEYLEFMPDNVIALGDFKRG
                     YYIVDHETGVRTRPDNLTEPGFIKIFTQKYLGGGVVDSNAIKILELPQDDD"
     gene            1974..2546
                     /locus_tag="ACHOSC_RS23405"
                     /old_locus_tag="pgaptmp_004681"
     CDS             1974..2546
                     /locus_tag="ACHOSC_RS23405"
                     /old_locus_tag="pgaptmp_004681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309618.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA];
                     GO_process: GO:0019082 - viral protein processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HK97 family phage prohead protease"
                     /protein_id="HGU4550460.1"
                     /translation="MGEKIMKSMEIRSSEITTSASNTLTGYVVRWDKLSELLWGEFYE
                     KFQRGAFTEWLAAGNDVRGLYEHDHSMLLGRTRSGTLKLEEDETGLRFELTPPDTSTG
                     RDVIELVKRGDISGMSFGFRSRKDVWDTTTDPCVRTVLVAELYEITVTSVPAYPDSGV
                     ELARRSLYEQHPEKMPRADNRRWWADLAGV"
     gene            2548..>2801
                     /locus_tag="ACHOSC_RS23410"
                     /old_locus_tag="pgaptmp_004682"
     CDS             2548..>2801
                     /locus_tag="ACHOSC_RS23410"
                     /old_locus_tag="pgaptmp_004682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HGU4550461.1"
                     /translation="MWPFRRKKEQRSMTLDEFMALAGTSNTGAGEYVSSGTAESLPAV
                     MNAVTVISEAVATMPCYLYLVRNEKGKEAREWLDSHPVDH"
BASE COUNT          749 a          658 c          768 g          626 t
ORIGIN      
        1 tgcaggaagg cacagaggcc accggagcca ccggataatt acggagacct gaaaaaggca
       61 gtcgatcgca cttatgccga aatggggata gatggtgctg gtgatgaaat atggcgcaat
      121 taccaggaca gctaatcaca cagccggaga aatccggctt tttttgcacc agttgaaacg
      181 gtatggcgca ttaccgggtt ttcgtcacgg tctggcatag ttactatctg aaacaaacag
      241 acacaacaga ggaaaaaaca atgccgatga aatttgatga gatattaaaa cagcgtgata
      301 aataccatgc tgacaacatg gagacgatga gcatcaatga ttaccgcgca ttcctggaga
      361 cgggcgcact gattgaaaag gatcagcatg gttttgttag atgtgctcta tccggtgaaa
      421 tgctggcggt aaatcctgaa cagatagatg cattgataga atttctgaaa gggatcaggg
      481 actgagcacg catacagccg gagcaatccg gctttttgtc attttttgta aattatttgt
      541 tcgtggttgt tccacgttgt tcactgacag gatcggcata ttttacccga actgaatcat
      601 gagtattctc gcccgtggtg ccaggacgct ggggccactt tcccgcctgt taatgtgctc
      661 gccaatattc attaccaggc gggaaaacga tcggtgcgat tactgatttc cttatgaaaa
      721 acggttgagt gtttgccgcg tcctggagtt ccttacttaa ccccaggact ttttttatgc
      781 cgagaataat cgaattacgc cagcagaaaa ccgccattaa aaatcagatg cgcgacatgc
      841 tggagaacgc ggaaaaagaa aaccgcagtc ttaacgatgc tgagggcgca aaatttgacg
      901 aattacgcgc taaagctgaa tccctcgata aagacatttc ccgccttgaa gccattgcag
      961 acgaagagcg cagcaagcca ggtaaaagca gtcagaccac tgaccccgca gaactacgca
     1021 actacattct gacaggtgaa acccgcgcat taagtacagg cgttcccgct gatggtggtt
     1081 ataccgttat ccctgaactg aacaccgaaa tcatgcgaat gctggcggat gaatccacca
     1141 tgcgccgcat ctgtaccgtg aagaaaatca gcagcaacga gtttaagcag cttgtttccg
     1201 ctggcggtgc gaccgttaac cacggtgaag agggtaaggc acgcgaacag accagccccc
     1261 cgcagattaa cgaggtgagc attaagctgt atccggtcta tgcgtacccg cgcaccacac
     1321 aggaaatcgt ggatttttcc gatgtggaca tcctttcatg gctgacgggt gagattggcg
     1381 acaccttcac ggaaaccgaa gaaagcgatc tggttgtggg cgacggtgac aaaaaagcaa
     1441 aaggtttttt atccgtaccc cgtgcagaga agaacgacaa agagcgtgat tttggtacgt
     1501 tgcaggtaat taaaccttcc gaatctctgg cgtggacatc tgcggacccg ctgatcgacc
     1561 tgaaatttgc attacgtaaa aaataccgca aaaacgcggt ctgggtggtt aactccacga
     1621 cggcggcaaa acttcagaag gtgaagaacg cgaacggtga ttatatctgg cgcgaccgtt
     1681 tacaggcggg tgatcctgat acgttgctgg gccttccggt cgaatatctg gagtttatgc
     1741 ctgataacgt tattgccctg ggtgacttca aacgcggtta ctacattgtt gatcacgaaa
     1801 caggtgttcg caccagaccg gacaacctca cagagccggg cttcatcaaa attttcacgc
     1861 agaaatattt aggcggtggc gtggtggatt cgaacgcgat caagattctg gaactgccac
     1921 aggacgacga ttaacagcat acagaagggg cttaaaagcc cctttagtgt tttatgggtg
     1981 aaaaaattat gaagagtatg gaaatccggt catcggaaat caccaccagc gccagcaaca
     2041 cgcttacagg ctacgttgtt cgctgggata agctttcaga gctgctatgg ggggagtttt
     2101 acgaaaaatt ccagcgggga gcgtttactg aatggcttgc ggcgggtaat gacgttcgcg
     2161 gcctgtatga gcatgaccac agcatgttgc tggggcgtac ccgttccggc acgctgaaac
     2221 tggaagagga cgaaacaggg ttacgctttg aactgacccc accggatacc agcacagggc
     2281 gggacgttat cgaactggtt aaacgtggtg atatatccgg catgagcttt ggctttcgtt
     2341 cccgtaagga tgtatgggat accacaacag atccatgcgt gcgcaccgtg ctggttgcgg
     2401 aactgtacga aattaccgtc acatccgtac cggcttaccc tgattccggc gtggagctgg
     2461 cccgccgctc cctgtacgag cagcaccccg aaaaaatgcc gcgtgcggat aatcgccgct
     2521 ggtgggcgga tttagcgggg gtgtgatatg tggcctttca gaagaaaaaa agagcagcgc
     2581 agcatgacgc ttgatgagtt tatggcgctg gctggcacat cgaacacggg ggcgggtgag
     2641 tacgtatcat cggggacagc ggaatcactg cccgccgtca tgaacgccgt gacggtcatc
     2701 tctgaggcgg tggctaccat gccgtgttac ctgtacctgg tacgcaatga gaaggggaag
     2761 gaggcccgcg agtggcttga ttctcatccg gtcgatcaca t
//
LOCUS       DAZKHF020000378         2780 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2201S31241
            SAMN43267800-rid25106413.denovo.380, whole genome shotgun sequence.
ACCESSION   DAZKHF020000378 DAZKHF020000000
VERSION     DAZKHF020000378.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267800
            Sequence Read Archive: SRR30307263
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2780)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2780)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 2780)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350581.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350581.2
            Genome Coverage       :: 151x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:21:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,652
            CDSs (total)                      :: 5,557
            Genes (coding)                    :: 5,320
            CDSs (with protein)               :: 5,320
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 237
            CDSs (without protein)            :: 237
            Pseudo Genes (ambiguous residues) :: 0 of 237
            Pseudo Genes (frameshifted)       :: 102 of 237
            Pseudo Genes (incomplete)         :: 130 of 237
            Pseudo Genes (internal stop)      :: 52 of 237
            Pseudo Genes (multiple problems)  :: 41 of 237
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2780
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267800-rid25106413.denovo.380"
                     /serotype="O 26:H11"
                     /isolate="2201S31241"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-01"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            complement(<1..1194)
                     /locus_tag="ACHOSC_RS23415"
                     /old_locus_tag="pgaptmp_004683"
     CDS             complement(<1..1194)
                     /locus_tag="ACHOSC_RS23415"
                     /old_locus_tag="pgaptmp_004683"
                     /EC_number="4.4.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000350897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_function: GO:0047804 - cysteine-S-conjugate beta-lyase
                     activity [Evidence IEA];
                     GO_process: GO:0009086 - methionine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MalY/PatB family protein"
                     /protein_id="HGU4550462.1"
                     /translation="MDIFNTSISRKGTYCTQWDFCEDRFGVKDVLPFSISDMDLPIPE
                     AIIRTLKKRLEHPILGYSRWQHDDYLNSIVNWYHQQYKTEIQPEWITYSPSVMYSISK
                     AIEILTLNGDNILIFTPVYNAFFDVIKHNGRNMLTTSLMKENGRGYVINWQDFELKIK
                     SAKMVLLCNPHNPTGTVWSEDDLHRIAACCARHNIWLCSDEIHSDFVFNHSFTSALTI
                     KDDKVVVFNSISKTFNVPALTGSYMISTDDNFNHKFRTISRYRDFVNSPSVLNVIATI
                     VAYNECEEWLKSLKVHIASNIQFTLQYLNENIPELIVWPAEGCYFSWVDCSALGYPFD
                     KFYRRLINEGRVGIMAGHVYGTEGEGYLRLNVACSREKLYMGLARLASVIKNINQEKE
                     NEQYGS"
     gene            complement(1194..2774)
                     /locus_tag="ACHOSC_RS23420"
                     /old_locus_tag="pgaptmp_004684"
     CDS             complement(1194..2774)
                     /locus_tag="ACHOSC_RS23420"
                     /old_locus_tag="pgaptmp_004684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000810568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HGU4550463.1"
                     /translation="MKQKKAWSFFQSLGKAFMYPIALLSVCGMMLGLGSGLASDDMGK
                     FIPVLAIPLIKTILDFIVSLGLFAFVNLPVLFAIAIPLGLLKEKDDKAYGAFSGLIGF
                     MAMHLGTNFYLKQHDLLVAADQMSTHGQTIILGIQSYNTSVLGGIVAGLLVASMYKKI
                     VNLRIPESLGFYSGPRLVPIITLIVMSGFGLFIPFIWPPFFNLFMLIGHWISTSGPVG
                     YFFYAVAERVTIPFGLNHLVTSVFRFTPIGGSAVIGGEEYYGTLNMFMAYVKENAVIP
                     LDLAGKMEQGKLMIQYGLAGAALAMYRTAHAQNRRAIKALLISGVLTVIIGGVSEPIE
                     FLFLFVSPLLFVFHAFMNGLANMVLPYMGVKMGFTGDLIQFISFGVLRGTRTGWPLAV
                     CVEVAYFFIYYFVFRWTILKFNLMTVGREESSPVTLNAHEETAIANIPVHDKSELQAA
                     EQMVKALGGKENIKSLDNCVTRLRLTIADMGLIDEAAIKRAGGIAVVKLDQNTLQVII
                     GTKVIALRRDMDNYMGIC"
BASE COUNT          868 a          646 c          495 g          771 t
ORIGIN      
        1 agatccgtat tgttcatttt ctttttcctg attaatattt ttaattacac tcgcaaggcg
       61 cgcaagcccc atatataatt tttctcgact gcaggccaca ttaagacgca gatagccctc
      121 tccttcagta ccataaacat gccccgccat aatacctact ctcccttcgt taattaatcg
      181 acggtaaaat ttatcaaagg gataaccaag ggccgagcaa tcaacccacg aaaaatagca
      241 gccctcagct ggccatacga tcaattccgg aatattttca tttaaatact gcaaggtaaa
      301 ttgaatgtta gaggcaatat gtacctttaa cgactttaac cattcttcac attcattata
      361 ggcaacaatg gtcgcaatta cattcagtac tgatggggag tttacgaaat cgcgatacct
      421 cgatatcgtt ctgaacttat gattaaaatt atcgtcagtt gaaatcatat atgatccggt
      481 tagcgcagga acattaaatg ttttcgaaat cgaattaaac accaccactt tgtcatcttt
      541 tattgttagc gctgacgtga aactatgatt aaaaacaaaa tcactatgta tttcatctga
      601 acataaccaa atattatgcc ttgcacagca tgctgcgatt ctgtgaagat cgtcttccga
      661 ccagaccgtt cctgtcgggt tatgaggatt acataataac accatcttcg cgcttttaat
      721 tttaagctca aagtcttgcc agttgataac gtagcctctc ccattttctt tcatcaaaga
      781 ggttgttagc atgtttcgac cattatgctt aatcacatca aaaaatgcgt tataaacagg
      841 cgtgaaaatg agaatattat ctccattcaa tgtcaatatt tctattgcct tagatattga
      901 atacatcacg cttgggctat aggtgatcca ttctggttga atctctgttt tatattgttg
      961 atgataccaa ttgactatcg aattcagata gtcatcatgc tgccaacgac tgtaccctaa
     1021 aatcgggtgt tccagacgtt tttttaatgt tctgatgata gcttccggta tcggaagatc
     1081 catatctgaa atagagaatg gtaatacatc ttttacacca aatctgtctt cacaaaagtc
     1141 ccattgtgtg caatatgttc ctttacgaga aattgaggta ttaaaaatat ccatcaacat
     1201 atccccatgt aattatccat atcccgacgt agggcgatga cttttgtacc gataatgact
     1261 tgtaaggtat tttgatcaag tttcacaacc gcaattcctc cggccctttt tattgcagcc
     1321 tcatctatca atcccatatc cgcgatagtt aaacgcaaac gagttacaca attatccagt
     1381 gatttaatat tttctttacc acccagagcc ttaaccattt gctccgctgc ctgcaactct
     1441 gatttatcat gaactgggat gttagctatt gcagtttctt cgtgagcgtt cagcgtgaca
     1501 ggactggact cttcacggcc tacagtcatc aggttaaatt tgagaatggt ccaacggaac
     1561 acaaagtaat aaatgaagaa ataagccact tcaacacata ccgctaatgg ccatcccgtt
     1621 cttgtgccac gtaagacacc aaagctaata aattgaatca gatcgccagt aaagcccatc
     1681 ttcacgccca tatatggcag aaccatgtta gccaatccat tcataaaggc atggaagaca
     1741 aacaacagcg ggctgacaaa taagaacaga aattcaatcg gctcgctgac gccgccaata
     1801 attaccgtga gcacaccgga aatcagcaat gctttgatag cccttctgtt ttgagcatga
     1861 gcagtgcgat acatcgccag cgcggctcca gccagaccat actgaatcat cagtttgccc
     1921 tgctccattt tccccgccaa atccagcgga atgaccgcat tctctttgac gtatgccata
     1981 aacatgttca gggtgccgta atactcttca ccgccaatca ctgccgatcc gccgattggg
     2041 gtaaagcgga aaactgatgt caccaagtga tttaagccaa aaggtatcgt cacgcgttcg
     2101 gcaacagcat agaagaaata accaacagga ccggaagttg aaatccagtg accaatgagc
     2161 atgaaaagat tgaaaaatgg cggccagata aaaggaatga acagaccaaa tccactcatc
     2221 acaatcagtg taatgatagg cacaaggcgt gggccactat aaaaccctaa tgattcagga
     2281 atacgtaagt taacgatctt tttatacatg ctggcgacta ataaccctgc cacaatccca
     2341 cccaacacgc tggtattgta ggactggatc ccgagtatga tggtttgccc atgtgtcgac
     2401 atttggtcag cagctaccaa taagtcgtgc tgtttaagat aaaagttcgt tccaagatgc
     2461 atcgccataa agccaattaa gccagaaaaa gcgccatagg ctttatcatc tttctctttt
     2521 aataatccca aaggtatagc tatcgcaaac aatacaggta aattaacgaa ggcaaacaaa
     2581 ccaaggctaa caataaaatc aagtatggtt ttaattaacg gaatagccaa cactggaatg
     2641 aattttccca tatcatcgct ggctaaacca cttcccagcc ctaacatcat accacataca
     2701 cttaacagag cgatgggata cataaatgcc ttccccaggc tctgaaaaaa actccaggct
     2761 ttcttttgtt tcatactggt
//
LOCUS       DAZKHF020000379         2759 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2201S31241
            SAMN43267800-rid25106413.denovo.381, whole genome shotgun sequence.
ACCESSION   DAZKHF020000379 DAZKHF020000000
VERSION     DAZKHF020000379.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267800
            Sequence Read Archive: SRR30307263
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2759)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2759)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 2759)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350581.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350581.2
            Genome Coverage       :: 151x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:21:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,652
            CDSs (total)                      :: 5,557
            Genes (coding)                    :: 5,320
            CDSs (with protein)               :: 5,320
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 237
            CDSs (without protein)            :: 237
            Pseudo Genes (ambiguous residues) :: 0 of 237
            Pseudo Genes (frameshifted)       :: 102 of 237
            Pseudo Genes (incomplete)         :: 130 of 237
            Pseudo Genes (internal stop)      :: 52 of 237
            Pseudo Genes (multiple problems)  :: 41 of 237
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2759
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267800-rid25106413.denovo.381"
                     /serotype="O 26:H11"
                     /isolate="2201S31241"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-01"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            complement(625..2085)
                     /gene="phoQ"
                     /locus_tag="ACHOSC_RS23425"
                     /old_locus_tag="pgaptmp_004685"
     CDS             complement(625..2085)
                     /gene="phoQ"
                     /locus_tag="ACHOSC_RS23425"
                     /old_locus_tag="pgaptmp_004685"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0016772 - transferase activity,
                     transferring phosphorus-containing groups [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     PhoQ"
                     /protein_id="HGU4550464.1"
                     /translation="MKKLLRLFFPLSLRVRFLLATAAVVLVLSLAYGMVALIGYSVSF
                     DKTTFRLLRGESNLFYTLAKWENNKLHVELPENIDKQSPTMTLIYDENGQLLWAQRDV
                     PWLMKMIQPDWLKSNGFHEIEADVNDTSLLLSGDHSIQQQLQEVREDDDDAEMTHSVA
                     VNVYPATSRMPKLTIVVVDTIPVELKSSYMVWSWFIYVLSANLLLVIPLLWVAAWWSL
                     RPIEALAKEVRELEEHNRELLNPATTRELTSLVRNLNRLLKSERERYDKYRTTLTDLT
                     HSLKTPLAVLQSTLRSLRSEKMSVSDAEPVMLEQISRISQQIGYYLHRASMRGGTLLS
                     RELHPVAPLLDNLTSALNKVYQRKGVNISLDISPEISFVGEQNDFVEVMGNVLDNACK
                     YCLEFVEISARQTDEHLYIVVEDDGPGIPLSKREVIFDRGQRVDTLRPGQGVGLAVAR
                     EITEQYEGKIVAGESMLGGARMEVIFGRQHSAPKDE"
     gene            complement(2085..2756)
                     /gene="phoP"
                     /locus_tag="ACHOSC_RS23430"
                     /old_locus_tag="pgaptmp_004686"
     CDS             complement(2085..2756)
                     /gene="phoP"
                     /locus_tag="ACHOSC_RS23430"
                     /old_locus_tag="pgaptmp_004686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator PhoP"
                     /protein_id="HGU4550465.1"
                     /translation="MRVLVVEDNALLRHHLKVQIQDAGHQVDDAEDAKEADYYLNEHL
                     PDIAIVDLGLPDEDGLSLIRRWRSNDVSLPILVLTARESWQDKVEVLSAGADDYVTKP
                     FHIEEVMARMQALMRRNSGLASQVISLPPFQVDLSRRELSINDEVIKLTAFEYTIMET
                     LIRNNGKVVSKDSLMLQLYPDAELRESHTIDVLMGRLRKKIQAQYPQEVITTVRGQGY
                     LFELR"
BASE COUNT          691 a          710 c          641 g          717 t
ORIGIN      
        1 cggcggtaac tcactgagat taaagcaaag cgcaaaattg cgcggtcggg cagaatgacc
       61 accacctcaa tcgtgacgga gagtgacaac cagtgacaca ccgtgacaaa aagacggttt
      121 aaccgttcat taaaaatcga cgtgattttg tgctttccgg atgacgtggc caacgtcatt
      181 ttttagcgaa aaattctgat taaggtcact ggctgaatgg tcataatgac cacgcagacc
      241 atcgaggcaa ccatagcgat catcgttacg gttggagctt ttccgccatt ggtggaaaag
      301 gtgcacatct ggatcatcat tacggtgatc aagggtatgt aaaacccacc agcctgatta
      361 aaggttaacc ggaaaaaaaa caggtggccc cgttatgggg atccccatat cgacattaac
      421 gcccctcatg aattggtgcg cttccccctg gaaagattac ccgcctgtat atttcttgtg
      481 tctatttgtt ccacgttgtt tcatacagtg caccgaacgg tgtagttaat ggtgtagtca
      541 atttgctgtt tttagcactt tttgcagtgt gagttgttta attaaatcaa ttggttgcct
      601 tgctgtggtg ataaaaggac atatttattc atctttcggc gcagaatgct ggcgaccaaa
      661 aatcacctcc atccgcgcac cgcccagcat gctctctccg gcgacgattt taccctcata
      721 ttgctcggtg atttcgcggg ctaccgccag ccctacacct tgcccagggc gtaaagtatc
      781 aacccgttga ccacggtcga aaatgacctc tcgcttgctt aatggaatac cggggccatc
      841 atcctcgacc acaatataga gatgctcgtc ggtttgcctt gcagaaattt cgacaaactc
      901 gaggcaatat ttacaggcat tatccagcac gttgcccatc acctcgacaa aatcgttctg
      961 ctcaccgaca aagctgatct ctggcgaaat atcgagagag atattgaccc ctttgcgttg
     1021 atacactttg ttcagcgctg aggtgagatt gtccagcagt ggggcgaccg gatgcagctc
     1081 gcggctgagc aatgtcccgc cgcgcatact ggcacgatgc aggtagtagc caatttgctg
     1141 tgaaatgcgg ctgatttgct ccagcattac cggctcagca tcactgacgc tcatcttttc
     1201 actacgcaga gaacgcagcg tactttgcag caccgccagt ggcgttttca gactatgggt
     1261 caggtcggtg agcgtcgtac ggtatttgtc gtaacgttcg cgttcacttt ttaacaatcg
     1321 gttcaggttt cgtaccagac tggtcagttc tcgcgttgtg gctggattga gcaattcgcg
     1381 gttatgttct tccagttcgc ggacttcttt tgccagggct tcgatggggc gtaaactcca
     1441 ccaggcggcg acccacagca gcgggatcac taacagcaga ttggctgaga gcacatagat
     1501 aaaccagctc cagaccatat aggaactttt tagctccacc ggaatggtat ccaccaccac
     1561 aatggttaat tttggcatcc gcgatgttgc cgggtagacg tttactgcca ccgagtgggt
     1621 catctccgcg tcgtcatcat cttcccgcac ttcctgcaac tgttgctgta tcgaatgatc
     1681 tccactcagc aagaggctgg tatcgttaac atccgcttca atttcatgaa aaccattcga
     1741 tttcagccag tcaggctgga tcatcttcat cagccagggc acgtcacgtt gcgcccataa
     1801 aagctgcccg ttctcatcat aaattagcgt catggtgggg ctttgcttgt cgatattttc
     1861 gggtaactcg acatgcaact tattgttttc ccacttcgca agggtataga acagattgct
     1921 ctcgccacgt aacagccgaa acgtagtttt atcgaaactg acgctataac cgatcagcgc
     1981 gaccattccg taggcaagcg aaagcaccag tactaccgca gccgttgcca acagaaaacg
     2041 tacccgcagt gagagcggga aaaaaagacg cagtaatttt ttcatcagcg caattcgaac
     2101 aaatagccct ggccgcgaac ggtagtaatc acttcttgcg gatattgtgc ctgaattttt
     2161 ttgcgcagac gtcccatcag tacatcaatg gtatggcttt cccgcagctc cgcatccgga
     2221 tagagttgga gcattaacga atctttgctc accactttgc cattattgcg tatcaacgtt
     2281 tccataatgg tgtattcgaa cgcggtcagt ttgatcactt cgtcattaat agataattca
     2341 cggcgagaga gatcaacctg aaacgggggt agcgaaatga cctgtgaagc caggccgcta
     2401 ttacgccgca ttaatgcctg cattcgcgcc atcacctctt caatatgaaa cggtttagtc
     2461 acgtaatcat cagcaccggc acttaatact tcgactttgt cctgccagct ttcacgggcg
     2521 gttaatacca gaatcggcag tgaaacatca ttgctacgcc agcggcgaat cagtgacaga
     2581 ccgtcctcgt caggcaatcc gagatcgaca atcgcaatat ccggtaaatg ttcattgaga
     2641 taataatcgg cttctttggc atcttctgcg tcatcgacct gatgaccagc atcctgaatc
     2701 tgaactttaa ggtggtgacg taacaacgca ttgtcttcaa caaccagtac gcgcatttt
//
LOCUS       DAZKHF020000380         2757 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2201S31241
            SAMN43267800-rid25106413.denovo.382, whole genome shotgun sequence.
ACCESSION   DAZKHF020000380 DAZKHF020000000
VERSION     DAZKHF020000380.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267800
            Sequence Read Archive: SRR30307263
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2757)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2757)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 2757)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350581.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350581.2
            Genome Coverage       :: 151x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:21:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,652
            CDSs (total)                      :: 5,557
            Genes (coding)                    :: 5,320
            CDSs (with protein)               :: 5,320
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 237
            CDSs (without protein)            :: 237
            Pseudo Genes (ambiguous residues) :: 0 of 237
            Pseudo Genes (frameshifted)       :: 102 of 237
            Pseudo Genes (incomplete)         :: 130 of 237
            Pseudo Genes (internal stop)      :: 52 of 237
            Pseudo Genes (multiple problems)  :: 41 of 237
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2757
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267800-rid25106413.denovo.382"
                     /serotype="O 26:H11"
                     /isolate="2201S31241"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-01"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            <1..1758
                     /gene="recE"
                     /locus_tag="ACHOSC_RS23435"
                     /old_locus_tag="pgaptmp_004687"
     CDS             <1..1758
                     /gene="recE"
                     /locus_tag="ACHOSC_RS23435"
                     /old_locus_tag="pgaptmp_004687"
                     /EC_number="3.1.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051908 - double-stranded DNA 5'-3' DNA
                     exonuclease activity [Evidence IEA];
                     GO_process: GO:0090304 - nucleic acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VIII"
                     /protein_id="HGU4550466.1"
                     /translation="YNLHPAHAKRIEEIIAENKPPFSVFRDKFITMPGGLDYSRAIVV
                     ASVKEAPIGIEVIPAHVTEYLNKVLTETDHANPDPEIVDIACGRSSAPMPQRVTEEGK
                     QDDEEKPQPSGAMADEQATAETVEPNATEHHQNTQPLDAQSQVNSVDAKYQELRAELH
                     EARKNIPSKNPVDADKLLAASRGEFVDGISDPNDPKWVKGIQTRDSVYQNQPETEKIS
                     PDAKQPEPVVQQEPEIVCNACGQTGGDNCPDCGAVMGDATYQETFGEENQVEAKEKDP
                     EEMEGAEHPHNENAGSDPHRDCSDETGEVADPVIVEDIEPGIYYGISNENYHAGPGVS
                     KSQLDDIADTPALYLWRKNAPVDTTKTKTLDLGTAFHCRVLEPEEFSNRFIVAPEFNR
                     RTNAGKEEEKAFLMECASTGKTVITAEEGRKIELMYQRVMALPLGQWLVESAGHAESS
                     IYWEDPETGILCRCRPDKIIPEFHWIMDVKTTADIQRFKTAYYDYRYHVQDAFYSDGY
                     EAQFGVLPTFVFLVASTTVECGRYPVEIFMMGEEAKLAGQQEYHRNLRTLADCLNTDE
                     WPAIKTLSLPRWAKEYAND"
     gene            1751..2560
                     /gene="recT"
                     /locus_tag="ACHOSC_RS23440"
                     /old_locus_tag="pgaptmp_004688"
     CDS             1751..2560
                     /gene="recT"
                     /locus_tag="ACHOSC_RS23440"
                     /old_locus_tag="pgaptmp_004688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006259 - DNA metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombination protein RecT"
                     /protein_id="HGU4550467.1"
                     /translation="MTKQPPIAKADLQKTQGNRAPAAVKNNDVISFINQPSMKEQLAA
                     ALPRHMTAERMIRIATTEIRKVPALGNCDTMSFVSAIVQCSQLGLEPGSALGHAYLLP
                     FGNKNEKSGKKNVQLIIGYRGMIDLARRSGQIASLSARVVREGDEFSFEFGLDEKLIH
                     RPGENEDAPVTHVYAVARLKDGGTQFEVMTRKQIELVRSQSKAGNNGPWVTHWEEMAK
                     KTAIRRLFKYLPVSIEIQRAVSMDEKEPLTIDPADSSVLTGEYSVIDNSEE"
     gene            2617..>2757
                     /gene="ralR"
                     /locus_tag="ACHOSC_RS23445"
                     /old_locus_tag="pgaptmp_004689"
     CDS             2617..>2757
                     /gene="ralR"
                     /locus_tag="ACHOSC_RS23445"
                     /old_locus_tag="pgaptmp_004689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system
                     endodeoxyribonuclease toxin RalR"
                     /protein_id="HGU4550468.1"
                     /translation="MRYDDVKPCPFCGCPSVTVKAISGYYRAKCNGCESRTGYGGSEK
                     EAL"
BASE COUNT          829 a          643 c          693 g          592 t
ORIGIN      
        1 tataaccttc atccggcaca cgctaaacgc attgaggaaa ttatcgctga aaataaacca
       61 cccttttctg ttttccgcga caaattcatc accatgcctg gcgggctgga ttattcccgc
      121 gccatcgtgg ttgcgtccgt gaaagaagca ccaattggga tcgaggtcat ccccgcacac
      181 gtcactgaat atctgaacaa agtactgact gaaaccgatc atgccaaccc tgatccggaa
      241 atcgtggata ttgcctgcgg tcgctcctcg gccccgatgc cgcaacgagt aacagaagaa
      301 ggaaaacagg acgatgaaga aaaaccacaa ccatctggcg caatggcaga tgaacaggca
      361 acggctgaaa cagtggaacc gaatgcaact gaacatcatc agaacacgca gccgctggat
      421 gctcagtcac aggtaaattc tgttgatgcg aaatatcagg aactgcgggc agaactccat
      481 gaagcccgga aaaacattcc atcaaaaaat cctgtcgatg ccgataaatt gcttgctgca
      541 tcacgtggtg aatttgttga cggaattagc gacccgaacg atccgaaatg ggttaagggg
      601 atccagactc gcgattctgt gtaccagaac cagccagaaa cggaaaaaat cagcccggat
      661 gcgaaacaac ctgagccagt agtgcaacag gaaccggaaa tagtctgcaa tgcctgcggt
      721 cagactggcg gggataactg ccctgactgt ggtgcggtga tgggcgacgc aacataccag
      781 gaaacattcg gtgaagagaa tcaggttgaa gctaaggaaa aagatccgga ggaaatggaa
      841 ggcgctgaac atccgcacaa tgagaatgct ggcagcgatc cgcatcgcga ttgcagtgat
      901 gaaactggcg aagtcgcaga tcccgtaatc gtagaagaca tagagccagg tatttattac
      961 ggaatttcga atgagaatta ccacgcgggt cccggcgtca gtaagtctca gctcgatgac
     1021 attgctgata ctccggcact gtatttgtgg cgtaaaaatg cccccgtgga cactacaaag
     1081 acaaaaacgc tcgatttagg aaccgctttc cactgccggg tacttgaacc ggaagaattc
     1141 agtaaccgct ttatcgtagc acctgaattt aaccgccgta caaacgccgg aaaagaagaa
     1201 gagaaagcgt ttctgatgga atgcgcaagc acaggaaaaa cggttatcac tgcggaagaa
     1261 ggccggaaaa ttgaactcat gtatcaacgc gttatggctt tgccgctggg gcaatggctt
     1321 gttgaaagcg ccggacacgc tgaatcatca atttactggg aagatccgga aacaggaatt
     1381 ttgtgtcggt gccgtccgga caaaattatt cctgaatttc actggatcat ggacgtgaaa
     1441 accacagcgg atattcaacg attcaaaacg gcttattacg actaccgcta tcacgttcag
     1501 gatgcattct acagtgacgg ttatgaagca cagtttggcg tgctgccaac tttcgttttt
     1561 ctggttgcca gcacaactgt tgaatgcgga cgttacccgg ttgagatttt catgatgggc
     1621 gaagaagcaa aactggcagg ccagcaggaa tatcaccgca atctgaggac cctggctgac
     1681 tgcctgaata ccgatgaatg gccagctatt aaaacgttat cactgccccg ctgggctaag
     1741 gagtatgcaa atgactaagc aaccacctat cgcaaaagcc gatctgcaaa aaacccaggg
     1801 aaaccgtgca ccagcagcag taaaaaataa cgacgtgatc agctttatta atcagccatc
     1861 aatgaaagag caactggcag cagctctccc acgccatatg acggctgaac gaatgatacg
     1921 tatcgccacc acagaaattc gtaaggttcc ggcgctagga aactgtgaca ccatgagttt
     1981 tgtcagtgcg atcgttcagt gttcacagct cggccttgag ccaggtagcg ccctcggcca
     2041 cgcatattta ctgccttttg gtaataaaaa cgaaaagagc ggtaaaaaga acgttcagct
     2101 aatcattggt tatcgcggca tgattgatct ggctcgccgt tctggtcaaa tcgccagcct
     2161 gtcagcccgt gttgtccgtg aaggtgacga gtttagtttt gaatttggcc ttgatgaaaa
     2221 gttaatacac cgcccgggag aaaacgaaga tgcaccagtg acccacgtct atgctgtcgc
     2281 aagactgaaa gacggaggga ctcagtttga agttatgacg cgcaaacaga ttgaactggt
     2341 gcgcagccag agtaaggctg gtaataacgg gccatgggta actcactggg aagaaatggc
     2401 aaaaaaaacg gctattcgtc gcctgtttaa atacctgcct gtctcaattg aaatccagcg
     2461 tgcagtatca atggatgaaa aggaaccact gacaatcgat ccggcagact cctctgtatt
     2521 aaccggggaa tacagtgtaa tcgataattc agaagaataa ttcagcctgg cggtgtaatg
     2581 caccgccaac gtgagacagt ttttatgaca aaaattatga gatatgacga tgttaaacca
     2641 tgtccgtttt gtggttgtcc atctgttaca gtgaaagcaa tttcaggata ttaccgggca
     2701 aaatgcaacg gatgcgaatc ccgaactggc tatggtggaa gtgaaaaaga agcactc
//
LOCUS       DAZKHF020000381         2747 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2201S31241
            SAMN43267800-rid25106413.denovo.383, whole genome shotgun sequence.
ACCESSION   DAZKHF020000381 DAZKHF020000000
VERSION     DAZKHF020000381.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267800
            Sequence Read Archive: SRR30307263
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2747)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2747)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 2747)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350581.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350581.2
            Genome Coverage       :: 151x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:21:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,652
            CDSs (total)                      :: 5,557
            Genes (coding)                    :: 5,320
            CDSs (with protein)               :: 5,320
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 237
            CDSs (without protein)            :: 237
            Pseudo Genes (ambiguous residues) :: 0 of 237
            Pseudo Genes (frameshifted)       :: 102 of 237
            Pseudo Genes (incomplete)         :: 130 of 237
            Pseudo Genes (internal stop)      :: 52 of 237
            Pseudo Genes (multiple problems)  :: 41 of 237
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2747
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267800-rid25106413.denovo.383"
                     /serotype="O 26:H11"
                     /isolate="2201S31241"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-01"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            <1..480
                     /locus_tag="ACHOSC_RS23450"
                     /old_locus_tag="pgaptmp_004690"
     CDS             <1..480
                     /locus_tag="ACHOSC_RS23450"
                     /old_locus_tag="pgaptmp_004690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PerC family transcriptional regulator"
                     /protein_id="HGU4550469.1"
                     /translation="LNEVQKFIEAHPGCTSGDIADAFAGYSRQRVLQSASKLRQSGRV
                     AHRCEGDTRRHFPRLTERAQEPEPQPVRETRPVRNFYVGTNDPRVILCLTRQAEELES
                     RGLYRRAATVWMAAFRESHSQPERNNFLARRERCLRKSSKRAASGEEWYLSGNYVGA"
     gene            480..1133
                     /locus_tag="ACHOSC_RS23455"
                     /old_locus_tag="pgaptmp_004691"
     CDS             480..1133
                     /locus_tag="ACHOSC_RS23455"
                     /old_locus_tag="pgaptmp_004691"
                     /EC_number="2.1.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001378271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0009007 - site-specific
                     DNA-methyltransferase (adenine-specific) activity
                     [Evidence IEA];
                     GO_process: GO:0009307 - DNA restriction-modification
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage N-6-adenine-methyltransferase"
                     /protein_id="HGU4550470.1"
                     /translation="MSNKYCQALVELRNKPAHELKEVGDQWRTPDNIFWGINTLFGPF
                     VLDLFTDGDNAKCAAYYTAEDNALAHDWSERLAELKGAAFGNPPYSRASQHEGQYITG
                     MRYIMKHASAMRDKGGRYVFLIKAATSEVWWPEDADHIAFIRGRIGFELPAWFIPKDE
                     KQVPTGAFFAGAIAVFDKTWKGPAISYIGRDELEACGEAFLAQVRQQAEKLVREMAA"
     gene            1130..1456
                     /locus_tag="ACHOSC_RS23460"
                     /old_locus_tag="pgaptmp_004692"
     CDS             1130..1456
                     /locus_tag="ACHOSC_RS23460"
                     /old_locus_tag="pgaptmp_004692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003878599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LexA family transcriptional regulator"
                     /protein_id="HGU4550471.1"
                     /translation="MTTLTQCQQQVLDMLISYQQERGFPPTNQEVATMLGYRSVNAAV
                     EHLRALEKKGVITIKRGVARGITLHTAVKDDDSEAVGIIRALLAGEENARLRAAHWLH
                     ERGLKV"
     gene            1453..1842
                     /locus_tag="ACHOSC_RS23465"
                     /old_locus_tag="pgaptmp_004693"
     CDS             1453..1842
                     /locus_tag="ACHOSC_RS23465"
                     /old_locus_tag="pgaptmp_004693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000767134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RusA family crossover junction
                     endodeoxyribonuclease"
                     /protein_id="HGU4550472.1"
                     /translation="MKLILPFPPSVNTYWRHPNKGAFAGKSLISAAGRKFQSAACAAI
                     VEQLRRLPKPTSAPASVEIVLFPPDNRIRDLDNYNKALFDALTHAGVWEDDSQVKRML
                     VEWGPVIPKGKVEITISKYEKTAGAAA"
     gene            1862..2671
                     /locus_tag="ACHOSC_RS23470"
                     /old_locus_tag="pgaptmp_004694"
     CDS             1862..2671
                     /locus_tag="ACHOSC_RS23470"
                     /old_locus_tag="pgaptmp_004694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001061418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005634 - nucleus [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="KilA-N domain-containing protein"
                     /protein_id="HGU4550473.1"
                     /translation="MNNLMVIDGIEVRRDAYGRYSLNDLHRAAGGEQKKRPKYWLSNK
                     QTCELIEQLFTEGGIPPLEKNQPVSVINGGNNQGTYVCKELVYAYAMWISPSFHLKVI
                     RTFDMVTSASEKLSGQAADKMQAGVILLDFMRRELNLSNSSVLGACQKLQEAVGLPNL
                     APRYAIDAPADAPDGSSRPTLSLSALLKQYGIRLTANQAYHQMVKLGIVEQRERYSRT
                     AINNIKKFWSLTAKGCMFGKNITSPANPRETQPHFFESRFPELLKLLDTVH"
BASE COUNT          672 a          670 c          812 g          593 t
ORIGIN      
        1 ttgaatgaag ttcagaaatt cattgaagcc catccgggat gtacttccgg agacattgcg
       61 gatgcttttg caggttactc acggcagcgc gttctgcagt cagcaagcaa gttacgtcag
      121 agtgggcgtg tggctcaccg ttgtgaagga gatacacgca gacatttccc gcgcctgact
      181 gagagagcgc aggaaccgga accacaacca gttcgagaaa ccagacctgt gcgcaatttc
      241 tatgtcggca ctaacgatcc acgggtgatt ttgtgcctga cccgccaggc tgaagaactg
      301 gagtccaggg gcttataccg tcgtgctgca acggtgtgga tggcggcatt ccgtgaaagc
      361 cactcccagc cagaacgaaa caattttctg gcgcgtcgtg agcggtgctt acggaaaagc
      421 agcaagcgcg ctgcatcggg tgaagagtgg tatctgtcag ggaattacgt gggggcttaa
      481 tgagtaataa atattgccag gcgctggtgg aactgcggaa caaaccagcc catgaactga
      541 aggaagtggg cgatcagtgg cgcacgccgg acaacatttt ctggggaatt aacaccctgt
      601 ttggcccgtt tgttctggat ctgtttactg acggtgataa cgccaaatgt gccgcgtatt
      661 acacggcgga agataacgcg ctggcgcatg actggtcaga acgtcttgcg gagcttaaag
      721 gtgctgcctt tggtaatccc ccatacagcc gcgccagtca gcatgagggg caatacatca
      781 ccggcatgcg ttacatcatg aaacatgcca gtgccatgcg tgataagggc gggcgctatg
      841 ttttcctgat caaagctgcc accagcgaag tgtggtggcc ggaagatgcg gaccatattg
      901 cttttattcg cgggcgtatt ggttttgaac tgcctgcctg gtttatcccg aaggatgaga
      961 agcaggtgcc gacaggcgct ttcttcgctg gtgctattgc tgttttcgac aagacctgga
     1021 agggaccggc aatcagctac atcgggcgcg atgaacttga ggcatgtggt gaggcctttc
     1081 tggcgcaggt tcgccagcag gcggaaaaac tggtcaggga gatggcggca tgacgacgtt
     1141 aactcaatgc cagcagcagg tgctggatat gctgatttct taccagcaag aacgtggctt
     1201 tccgccaacc aatcaggagg tggcaaccat gctgggatac cgttcggtga atgcagcggt
     1261 ggagcatctt cgcgcactgg agaaaaaagg cgtcatcacg ataaagcgtg gcgtggcccg
     1321 ggggataact cttcataccg cggtgaagga cgacgacagc gaggcggtcg ggattatccg
     1381 cgcactgctt gccggtgagg aaaacgccag gctgcgtgca gcccactggt tacatgagag
     1441 gggcctgaaa gtatgaagct gatcttgcct ttccctccca gcgtgaacac gtactggcga
     1501 caccccaaca aaggggcgtt tgctggtaag agtctgataa gcgcagcggg gcgcaaattc
     1561 cagagcgcgg cgtgcgcagc aatagttgag cagttacgtc gtctgccgaa accaacgtcg
     1621 gcacctgctt cagtggagat cgtgttgttt cctccggata accggatccg cgatctggac
     1681 aactataaca aggcgctgtt tgacgccctg acccatgcgg gtgtgtggga agacgacagt
     1741 caggtgaaaa gaatgctggt ggagtgggga ccggttatcc cgaaagggaa ggtcgagatc
     1801 actatcagta agtacgagaa aacggcgggt gcagccgcct gatcaagagg agaaacgaag
     1861 tatgaataat ctgatggtca ttgatggtat tgaagttcgt cgtgatgctt atgggcgtta
     1921 cagcctgaac gatctgcaca gggcagccgg gggagaacaa aaaaaacgcc cgaaatactg
     1981 gctctccaat aagcaaacct gtgaattgat tgaacaactt ttcaccgagg gtggaattcc
     2041 gcctctggaa aagaatcaac cagttagcgt tattaatggc ggaaataacc aggggacgta
     2101 tgtctgcaaa gaactggtgt atgcctatgc aatgtggatc agcccgtcat tccatctgaa
     2161 ggtgatccgt actttcgata tggtaaccag cgcatcggaa aaattatccg ggcaggctgc
     2221 tgacaagatg caggctggcg tgattctgct ggactttatg cgccgggagt taaacctgtc
     2281 taactcttca gtgcttggtg cctgtcagaa actccaggag gctgttggct taccgaatct
     2341 ggcaccgcgc tatgccattg atgctcctgc tgacgcgcct gatggctcaa gccgtcccac
     2401 gctgtcactg agtgcactgc tgaagcagta tggtatccgc ctgacagcta atcaggcata
     2461 tcaccagatg gtgaagctgg ggatcgtcga gcagcgcgaa cgatacagcc gtaccgcgat
     2521 taacaacatc aaaaaattct ggtcgctgac agcgaaaggc tgcatgttcg gcaagaacat
     2581 caccagtccc gcaaatccgc gcgagacgca gccgcatttc ttcgaatccc gattccctga
     2641 gctgttaaag ctgctcgata ccgttcattg aggtgaccgt gagagcacta ctgacccctg
     2701 aaattgcccc gcgtatgggg atcgtattgt tcaggccagg ttcagag
//
LOCUS       DAZKHF020000382         2737 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2201S31241
            SAMN43267800-rid25106413.denovo.384, whole genome shotgun sequence.
ACCESSION   DAZKHF020000382 DAZKHF020000000
VERSION     DAZKHF020000382.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267800
            Sequence Read Archive: SRR30307263
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2737)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2737)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 2737)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350581.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350581.2
            Genome Coverage       :: 151x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:21:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,652
            CDSs (total)                      :: 5,557
            Genes (coding)                    :: 5,320
            CDSs (with protein)               :: 5,320
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 237
            CDSs (without protein)            :: 237
            Pseudo Genes (ambiguous residues) :: 0 of 237
            Pseudo Genes (frameshifted)       :: 102 of 237
            Pseudo Genes (incomplete)         :: 130 of 237
            Pseudo Genes (internal stop)      :: 52 of 237
            Pseudo Genes (multiple problems)  :: 41 of 237
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2737
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267800-rid25106413.denovo.384"
                     /serotype="O 26:H11"
                     /isolate="2201S31241"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-01"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            complement(65..823)
                     /locus_tag="ACHOSC_RS23475"
                     /old_locus_tag="pgaptmp_004695"
     CDS             complement(65..823)
                     /locus_tag="ACHOSC_RS23475"
                     /old_locus_tag="pgaptmp_004695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HGU4550474.1"
                     /translation="MSQILLQPANAMITLENVNKWYGQFHVLKNINLTVQPGERIVLC
                     GPSGSGKSTTIRCINHLEEHQQGRIVVDGIELNEDIRNIERVRQEVGMVFQHFNLFPH
                     LTVLQNCTLAPIWVRKMPKKEAEALAMHYLERVRIAEHAHKFPGQISGGQQQRVAIAR
                     SLCMKPKIMLFDEPTSALDPEMVKEVLDTMIGLAQSGMTMLCVTHEMGFARTVADRVI
                     FMDRGEIVEQAAPDEFFTHPKSERTRAFLSQVIH"
     gene            complement(831..1934)
                     /gene="yhdY"
                     /locus_tag="ACHOSC_RS23480"
                     /old_locus_tag="pgaptmp_004696"
     CDS             complement(831..1934)
                     /gene="yhdY"
                     /locus_tag="ACHOSC_RS23480"
                     /old_locus_tag="pgaptmp_004696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709065.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HGU4550475.1"
                     /translation="MTKVLLSQPSRPASHNSSRAMVWVRKNLFSSWSNSLLTIGCIWL
                     MWELIPPLLNWAFLQANWVGSTRADCTKAGACWVFIHERFGQFMYGLYPHDQRWRINL
                     ALLIGLVSIAPMFWKILPHRGRYIAVWAVIYPLIVWWLMYGGFFGLERVETRQWGGLT
                     LTLIIASVGIAGALPWGILLALGRRSHMPIVRILSVIFIEFWRGVPLITVLFMSSVML
                     PLFMAEGTSIDKLIRALVGVILFQSAYVAEVVRGGLQALPKGQYEAAESLALGYWKTQ
                     GLVILPQALKLVIPGLVNTIIALFKDTSLVIIIGLFDLFSSVQQATVDPTWLGMSTEG
                     YVFAALIYWIFCFSMSRYSQHLEKRFNTGRTPH"
     gene            complement(1944..>2737)
                     /locus_tag="ACHOSC_RS23485"
                     /old_locus_tag="pgaptmp_004697"
     CDS             complement(1944..>2737)
                     /locus_tag="ACHOSC_RS23485"
                     /old_locus_tag="pgaptmp_004697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HGU4550476.1"
                     /translation="FRNIPPLLQIFFWYFAVLRNLPGPRQAVSAFDLAFLSNRGLYIP
                     SPQLGDGFIAFILAVVMAIILSVGLFRFNKTYQIKTGQLRRTWPIAAVLIIGLPLLAQ
                     WLFGAALHWDVPALRGFNFRGGMVLIPELAALTLALSVYTSAFIAEIIRAGIQAVPYG
                     QHEAARSLGLPNPVTLRQVIIPQALRVIIPPLTSQYLNIVKNSSLAAAIGYPDMVSLF
                     AGTVLNQTGQAIETIAMTMSVYLIISLTISLLMNIYNRRIAIVER"
BASE COUNT          755 a          733 c          649 g          600 t
ORIGIN      
        1 catcaaatca agcaaaaagg ccatccgaaa ggatggcctt tttgcttttc gaactaacat
       61 tcaattaatg gattacctgc gataaaaatg ccctcgtacg ctctgattta ggatgcgtaa
      121 aaaattcatc aggtgcagct tgctccacta tttccccacg atccataaaa atcacccggt
      181 cagcgacggt tcgcgcaaac cccatctcat gcgttacaca caacattgtc atacccgatt
      241 gcgccagccc aatcatcgta tccagcacct ctttcaccat ctccggatcg agcgccgacg
      301 ttggctcatc aaacaacata attttcggct tcatacacag cgaacgcgca atggcaacgc
      361 gttgctgctg accaccagaa atttgtccgg gaaacttatg cgcatgttcg gcaattctca
      421 cccgctcaag gtaatgcatc gccagagctt cagcctcttt ctttggcatc ttgcgtaccc
      481 aaatcggtgc cagggtacag ttctgtagaa cggtcagatg agggaagaga ttgaaatgct
      541 gaaagaccat tcccacttcc tgccggacgc gctcaatatt gcggatatct tcattaagtt
      601 cgatgccatc taccacgatc cgtccctgtt gatgttcttc cagatgatta atacaacgaa
      661 tggttgtcga tttaccggaa cctgaagggc cacacagaac gatccgttct cccggttgca
      721 cggttaaatt tatatttttc aaaacatgga attgtccata ccatttattg acgttttcca
      781 gcgtaatcat cgcgttagca ggttgcagta aaatttggct catagtgtcc tcaatgcggt
      841 gtacgcccgg tgttaaaacg tttttccaga tgctggctat agcgcgacat gctgaaacag
      901 aagatccagt agatcagcgc ggcgaaaaca tacccttccg tcgacatacc caaccaggtg
      961 ggatcaacgg ttgcctgctg aacgctactg aaaagatcga acaacccgat aatgatcacc
     1021 aggctggtat ctttgaagag tgcgatgatg gtatttacca gcccaggaat taccagcttc
     1081 aacgcctgtg gcagaataac cagcccctga gttttccagt aacccaacgc cagcgactct
     1141 gccgcttcat actgcccttt aggcagtgcc tgtaatccgc ctcgcacgac ttccgcaaca
     1201 tatgctgact gaaacaggat cacgccaacc agcgcgcgga tcagtttgtc gatactggtg
     1261 ccttctgcca taaacaacgg cagcatgacc gaagacataa acaatacggt aatcagcggt
     1321 acgccgcgcc agaactcgat aaaaatgacc gataagatac gcacaatcgg catatgggag
     1381 cggcgaccta acgccagtaa gatcccccac ggtagcgccc ccgcaatccc aacggatgca
     1441 ataattaaag ttagcgtcag cccgccccat tgccgggttt caacccgctc cagaccaaaa
     1501 aaaccgccat acatcagcca ccagacaatc agtgggtaaa tcaccgccca gaccgcaata
     1561 tagcgaccgc gatgcgggag tattttccag aacattggtg cgattgacac aaggccaatc
     1621 agtaatgcca ggttaattcg ccagcgttgg tcgtgtgggt aaagtccata cataaactga
     1681 ccaaatcgtt cgtggatgaa gacccaacag gcaccggctt ttgtgcagtc ggcacgcgtt
     1741 gagccaaccc aattagcctg caaaaatgcc cagttcagca acggtgggat cagttcccac
     1801 atcaaccata tgcagccaat agtcagcagg ctattgctcc agctggagaa cagatttttt
     1861 cgcacccaca ccatcgcgcg gcttgagtta tggctcgctg ggcgcgaggg ctgagacagc
     1921 aatacttttg tcatcatgac tccttagcgt tcaacgatcg cgatgcggcg gttatagata
     1981 ttcatcagca gcgagatagt caggctgata atcagataga ccgacatggt catggctatc
     2041 gtctcgatgg cttgccccgt ctggttcagc acggtgccag caaacagcga aaccatatcg
     2101 ggatagccaa tagcggcggc aagagaggag tttttgacga tgttgagata ctggctggtt
     2161 aacggcggaa taatcactcg cagtgcctgg ggaataatga cctggcgtag cgtaaccggg
     2221 ttgggtaatc ccagtgaccg agccgcttca tgttgaccat aaggcactgc ctggatccca
     2281 gcgcggataa tctcggcgat aaatgcagat gtataaaccg aaagtgccag cgttaaggct
     2341 gccagttcag gaattaaaac catcccgccg cggaagttaa agcctcgtag ggctgggaca
     2401 tcccagtgta gtgctgcgcc aaaaagccat tgcgccagta aaggcaaacc aatgatcaac
     2461 actgcggcga tcggccaggt gcggcgcagt tgtccggtct ttatctggta tgttttatta
     2521 aagcggaata gcccaacaga aaggattata gccataacaa cagccaggat aaacgcaata
     2581 aatccgtctc ccagctgcgg tgacggaata taaagcccac gattgctcaa aaaggccaga
     2641 tcaaacgcgc tcacagcctg gcgtggtccg ggcaaattgc gcaacacggc aaagtaccag
     2701 aagaagattt gcagcagcgg gggaatatta cggaaga
//
LOCUS       DAZKHF020000383         2713 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2201S31241
            SAMN43267800-rid25106413.denovo.385, whole genome shotgun sequence.
ACCESSION   DAZKHF020000383 DAZKHF020000000
VERSION     DAZKHF020000383.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267800
            Sequence Read Archive: SRR30307263
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2713)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2713)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 2713)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350581.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350581.2
            Genome Coverage       :: 151x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:21:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,652
            CDSs (total)                      :: 5,557
            Genes (coding)                    :: 5,320
            CDSs (with protein)               :: 5,320
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 237
            CDSs (without protein)            :: 237
            Pseudo Genes (ambiguous residues) :: 0 of 237
            Pseudo Genes (frameshifted)       :: 102 of 237
            Pseudo Genes (incomplete)         :: 130 of 237
            Pseudo Genes (internal stop)      :: 52 of 237
            Pseudo Genes (multiple problems)  :: 41 of 237
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2713
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267800-rid25106413.denovo.385"
                     /serotype="O 26:H11"
                     /isolate="2201S31241"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-01"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            complement(78..1484)
                     /gene="gndA"
                     /locus_tag="ACHOSC_RS23490"
                     /old_locus_tag="pgaptmp_004698"
     CDS             complement(78..1484)
                     /gene="gndA"
                     /locus_tag="ACHOSC_RS23490"
                     /old_locus_tag="pgaptmp_004698"
                     /EC_number="1.1.1.44"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004616 - phosphogluconate dehydrogenase
                     (decarboxylating) activity [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent phosphogluconate dehydrogenase"
                     /protein_id="HGU4550477.1"
                     /translation="MSKQQIGVVGMAVMGRNLALNIESRGYTVSIFNRSREKTEEVIA
                     ENPGKKLVPYYTVKEFVESLETPRRILLMVKAGAGTDAAIDSLKPYLDKGDIIIDGGN
                     TFFQDTIRRNRELSAEGFNFIGTGVSGGEEGALKGPSIMPGGQKEAYELVAPILTKIA
                     AVAEDGEPCVTYIGADGAGHYVKMVHNGIEYGDMQLIAEAYSLLKGGLNLSNEELAQT
                     FTEWNNGELSSYLIDITKDIFTKKDEDGNYLVDVILDEAANKGTGKWTSQSALDLGEP
                     LSLITESVFARYISSLKDQRVAASKVLSGPQAQPAGDKAEFIEKVRRALYLGKIVSYA
                     QGFSQLRAASEEYNWDLNYGEIAKIFRAGCIIRAQFLQKITDAYAENPQIANLLLAPY
                     FKQIADDYQQALRDVVAYAVQNGIPVPTFAAAVAYYDSYRAAVLPANLIQAQRDYFGA
                     HTYKRIDKEGVFHTEWLD"
     gene            complement(1595..2002)
                     /locus_tag="ACHOSC_RS23495"
                     /old_locus_tag="pgaptmp_004699"
     CDS             complement(1595..2002)
                     /locus_tag="ACHOSC_RS23495"
                     /old_locus_tag="pgaptmp_004699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001241508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WbuC family cupin fold metalloprotein"
                     /protein_id="HGU4550478.1"
                     /translation="MRLVDDNIMDELFRTAANSERLRAHYLLHASHQEKVQRLLIAFV
                     RDSYVEPHWHELPHQWEMFVVMQGQLEVCLYEQNGELQKKFVVGDGTGISVVEFSPGD
                     IHSVKCLSPKALMLEIKEGPFDPLKAKAFSKWL"
     gene            complement(1993..>2713)
                     /locus_tag="ACHOSC_RS23500"
                     /old_locus_tag="pgaptmp_004700"
     CDS             complement(1993..>2713)
                     /locus_tag="ACHOSC_RS23500"
                     /old_locus_tag="pgaptmp_004700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001516617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HGU4550479.1"
                     /translation="QADWIGLMSDKNLEIFRQANKGYPCEVLRNWASMTPVSAGDDYH
                     SLRQKYALKDKIIFFYGGNIGHAQDMANLMRLARNMMRHHDAHFLFIGQGDEVDLIKS
                     LSAEWNLTNFTHLPSVNQEEFKLILSEVDVGLFSLSSRHSSHNFPGKLLGYMVQSIPI
                     LGSVNGGNDLMDVINKHRAGFIHVNGEDDKLFESAQLLLSDSVLRKQLGQNANVLLKS
                     QFSVESAAHTIEVRLEAGECV"
BASE COUNT          770 a          654 c          586 g          703 t
ORIGIN      
        1 gtctataaga taattggcaa aaaaaaaagc ccggcgtaat atacgccggg cctcaattta
       61 ttgttggtta aatcagatta atccagccat tcagtatgga acacaccttc tttatcaatg
      121 cgcttataag tatgcgcacc aaaatagtca cgctgagcct ggatcaggtt cgcaggcaga
      181 acagcggcac ggtagctgtc ataataggca accgcagcgg cgaaggtcgg aaccgggata
      241 ccgttctgta ctgcataagc aacgacatca cgcagcgcct gctggtagtc atcggcaatt
      301 tgcttgaagt acggagccaa cagcaggtta gcgatctgag gattttcggc ataagcatca
      361 gttattttct gcaggaactg cgcacggatg atgcagccag cacggaaaat cttcgcgatt
      421 tcgccgtagt tcagatccca gttgtactct tcagacgctg cgcgcagctg agagaagccc
      481 tgagcgtaag aaacgatttt gcccagatac agcgcacggc gaactttttc gatgaactca
      541 gccttgtcgc cagctggctg cgcttgcggg ccagagagaa ctttagatgc ggcaacacgc
      601 tgatctttca gagaagagat ataacgtgca aacacagact cggtaatcag cgacagcggt
      661 tcaccgagat ccagcgcgct ctggctggtc catttaccgg tacctttgtt agccgcttca
      721 tccaggatca catcaaccag gtagttaccg tcttcatctt ttttggtgaa gatgtctttg
      781 gtgatgtcga tcaggtagct actcagttca ccgttattcc actcggtaaa ggtctgtgcc
      841 agttcttcgt tagagagatt caggccgcct ttcaggagag aataggcttc agcaatcagt
      901 tgcatatcac cgtattcaat accgttgtga accatcttca catagtgacc cgcgccatca
      961 gcaccaatat aggtaacgca cggttcgcca tcttcagcaa cggcggcgat tttagtcagg
     1021 atcggtgcaa ccagttcata ggcttctttc tgcccaccag gcataatgga aggacctttc
     1081 agtgcgcctt cttcaccacc ggagacaccg gtaccaatga agttaaaacc ttctgcagaa
     1141 agctcacgat tacgacgaat ggtgtcctgg aagaaggtat taccaccatc aatgatgatg
     1201 tcacctttat cgaggtatgg cttaagggaa tcaatagcag catccgtgcc tgcacctgct
     1261 ttcaccatta acaggatgcg acgaggcgtt tccagagatt caacaaactc tttcaccgta
     1321 tagtaaggaa ccagtttctt gcctggattt tcggcaatca cttcttccgt cttttcacgg
     1381 gaacggttga aaatagagac ggtataacca cggctttcga tgttgagcgc aaggttgcgc
     1441 cccatcactg ccataccgac tacgccgatc tgttgctttg acattgttta ctcctgtcag
     1501 gggtggtcac gcggggttac gcggcttgaa gaagctaaga tggtaaccca gcatgtatag
     1561 aataagatag aagaataaac ggtggtgtat cgccctataa ccacttagaa aaagccttag
     1621 ctttcagtgg gtcaaatggc ccctccttta tctctaacat aagggctttt ggtgacaggc
     1681 atttgacact atgtatatct cctggggaaa attccacgac acttattccc gtaccgtctc
     1741 caacaacaaa ctttttttgg agctcaccat tttgctcata caaacaaact tctaattgcc
     1801 cttgcatgac gacaaacatt tcccactgat gcggtaactc atgccaatgg ggttcaacat
     1861 agctgtcgcg tacaaatgca ataagtaaac gttgaacctt ctcctgatga gatgcgtgca
     1921 ataaataatg agcgcgcaaa cgttcagaat ttgctgcagt gcgaaaaagt tcatccataa
     1981 tattgtcatc aactaaacgc attctcctgc ctccagtcgg acttcgatag tatgtgccgc
     2041 cgattcaacc gaaaattgag actttaacaa cacattggcg ttctgaccta actgttttct
     2101 taaaactgaa tcactaagaa gcaattgtgc agattcaaac agtttatcat cttcaccatt
     2161 aacatgaatg aacccagccc tgtgcttatt aattacatcc attagatcat taccgccatt
     2221 cacactccca aggatcggga ttgattgaac catataccct agtaattttc cggggaaatt
     2281 atgtgaagaa tggcgagacg aaagggagaa caggccaaca tcaacttcag ataaaattaa
     2341 tttaaactct tcctggttca ctgaaggtag atgagtgaaa ttagttaaat tccattctgc
     2401 agaaagagat ttaatcaggt caacttcatc accctgccct ataaacagga aatgagcatc
     2461 atgatgacgc atcatattac gcgcaaggcg cattaagttt gccatatcct gagcatgccc
     2521 aatattaccg ccatagaaaa aaatgatttt atcttttaga gcgtattttt gacgaagtga
     2581 atgataatca tcgccggcag acacaggagt cattgaggcc caattacgta aaacttcaca
     2641 cggataacct ttattggcct gacgaaatat ctcaagattc ttatcagaca ttaacccaat
     2701 ccagtcagcc tgt
//
LOCUS       DAZKHF020000384         2693 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2201S31241
            SAMN43267800-rid25106413.denovo.386, whole genome shotgun sequence.
ACCESSION   DAZKHF020000384 DAZKHF020000000
VERSION     DAZKHF020000384.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267800
            Sequence Read Archive: SRR30307263
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2693)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2693)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 2693)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350581.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350581.2
            Genome Coverage       :: 151x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:21:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,652
            CDSs (total)                      :: 5,557
            Genes (coding)                    :: 5,320
            CDSs (with protein)               :: 5,320
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 237
            CDSs (without protein)            :: 237
            Pseudo Genes (ambiguous residues) :: 0 of 237
            Pseudo Genes (frameshifted)       :: 102 of 237
            Pseudo Genes (incomplete)         :: 130 of 237
            Pseudo Genes (internal stop)      :: 52 of 237
            Pseudo Genes (multiple problems)  :: 41 of 237
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2693
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267800-rid25106413.denovo.386"
                     /serotype="O 26:H11"
                     /isolate="2201S31241"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-01"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            complement(89..1279)
                     /gene="aspC"
                     /locus_tag="ACHOSC_RS23505"
                     /old_locus_tag="pgaptmp_004701"
     CDS             complement(89..1279)
                     /gene="aspC"
                     /locus_tag="ACHOSC_RS23505"
                     /old_locus_tag="pgaptmp_004701"
                     /EC_number="2.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009653304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate transaminase"
                     /protein_id="HGU4550480.1"
                     /translation="MFENITAAPADPILGLADLFRADERPGKINLGIGVYKDETGKTP
                     VLTSVKKAEQYLLENETTKNYLGIDGIPEFGRCTQELLFGKGSALINDKRARTAQTPG
                     GTGALRVAADFLAKNTSVKRVWVSNPSWPNHKSVFNSAGLEVREYAYYDAENHTLDFD
                     ALINSLNEAQAGDVVLFHGCCHNPTGIDPTLEQWQTLAQLSVEKGWLPLFDFAYQGFA
                     RGLEEDAEGLRAFAAMHKELIVASSYSKNFGLYNERVGACTLVAADSETVDRAFSQMK
                     AAIRANYSNPPAHGASVVATILSNDALRAIWEQELTDMRQRIQRMRQLFVNTLQEKGA
                     NRDFSFIIKQNGMFSFSGLTKEQVLRLREEFGVYAVASGRVNVAGMTPDNMALLCEAI
                     VAVL"
     gene            complement(1464..2552)
                     /gene="ompF"
                     /locus_tag="ACHOSC_RS23510"
                     /old_locus_tag="pgaptmp_004702"
     CDS             complement(1464..2552)
                     /gene="ompF"
                     /locus_tag="ACHOSC_RS23510"
                     /old_locus_tag="pgaptmp_004702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porin OmpF"
                     /protein_id="HGU4550481.1"
                     /translation="MMKRNILAVIVPALLVAGTANAAEIYNKDGNKVDLYGKAVGLHY
                     FSKGNGENSYGGNGDMTYARLGFKGETQINSDLTGYGQWEYNFQGNNSEGADAQTGNK
                     TRLAFAGLKYADVGSFDYGRNYGVVYDALGYTDMLPEFGGDTAYSDDFFVGRVGGVAT
                     YRNSNFFGLVDGLNFAVQYLGKNERDTARRSNGDGVGGSISYEYEGFGIVGAYGAADR
                     TNLQEAQPLGNGKKAEQWATGLKYDANNIYLAANYGETRNATPITNKFTNTSGFANKT
                     QDVLLVAQYQFDFGLRPSIAYTKSKAKDVEGIGDVDLVNYFEVGATYYFNKNMSTYVD
                     YIINQIDSDNKLGVGSDDTVAVGIVYQF"
BASE COUNT          670 a          665 c          647 g          711 t
ORIGIN      
        1 aaggcctgat aagcgtagcg catcaggcaa tgttgcgttt gtcatcagtc tcagcccgct
       61 tttcagcggg cttcattgtt tttaatgctt acagcactgc cacaatcgct tcgcacagca
      121 gagccatgtt atctggtgtc atcccggcca catttacgcg accagaagca accgcatata
      181 cgccaaactc ttcgcgcaga cgcagcactt gttcttttgt caggccactg aaggagaaca
      241 tgccgttctg tttgatgata aagctgaagt cgcggtttgc gcctttttcc tgcagcgtat
      301 tgacgaacaa ctgacgcata cgctgaatac gctggcgcat atcagtcagc tcttgttccc
      361 aaatcgcacg taacgcatcg ttgctcagga tggtggcaac aacagaagcg ccgtgtgctg
      421 gtgggttaga gtagttagcg cgaatcgccg ctttcatttg gctgaatgcg cgatcaacgg
      481 tttcactgtc ggcagcaacc agagtacaag cgccaacacg ctcgttgtac aggccaaagt
      541 ttttagagta ggaactggca acaatcagct ctttatgcat agccgcgaaa gcgcgcagtc
      601 cttcagcatc ttcttccaga ccacgggcaa aaccctggta agcgaagtca aacagcggta
      661 accagccttt ctcaactgag agttgtgcca gtgtctgcca ttgttccagc gtagggtcga
      721 taccggttgg gttatggcag cagccatgga acagcactac gtcgccagcc tgagcttcgt
      781 tcaggctgtt aatcagtgca tcgaagtcaa gggtgtggtt ttctgcatca taataagcgt
      841 attcacgaac ttccagacct gcagagttaa agacgctctt atggttcggc cagcttgggt
      901 tgctcaccca cactcgctta acgctggtat tttttgccag gaaatcggca gctacgcgta
      961 gtgcgccagt gccccccgga gtctgtgccg tgcgagcacg tttgtcattg atcagggcgc
     1021 tacctttacc aaacagcagt tcctgagtgc agcgaccaaa ttcagggatg ccgtcaatgc
     1081 cgaggtaatt tttggtggtt tcattttcga gcagatactg ttcagccttt ttcacgctgg
     1141 tcaataccgg ggttttgccc gtctcatctt tatagacacc aatcccgagg ttaattttgc
     1201 cgggacgttc atcggcacga aacagatcgg ccaggcccag aatcgggtcg gcaggagcgg
     1261 cggtaatgtt ctcaaacatg acgaggttcc attatggtta cagaagggaa gtccgctatc
     1321 agggtaacgg gagatttaca aaattccaac tattactgat gaaaacgcag gctgtttttg
     1381 caagacgtga gattgctctg gaaggcataa aaaaaacagg accaaagtcc tgttttttcg
     1441 gcatttaaca aagaggtgtg ctattagaac tggtaaacga tacccacagc aacggtgtcg
     1501 tcagaaccta cgcccagttt gttgtcagaa tcgatctggt tgatgatgta gtcaacatag
     1561 gtggacatgt ttttgttgaa gtagtaggtt gcgcccactt caaagtagtt caccagatca
     1621 acatcaccga taccttctac gtctttcgct ttagatttgg tgtaagcgat ggacggacgc
     1681 agaccgaaat cgaactggta ttgcgcaact aacagaacgt cttgcgtttt gttggcgaag
     1741 ccgctggtgt ttgtaaattt attagtgatc ggcgtagcgt tacgggtttc accgtagttc
     1801 gctgccaggt agatgttgtt cgcgtcgtac ttcagaccag tagcccactg ttcagctttt
     1861 ttaccgttgc caagaggttg agcttcttgc aggttggtac ggtcagctgc accataagca
     1921 ccaacgatac caaagccttc gtattcgtag ctgatagaac cgccaacacc gtcgccgtta
     1981 gaacggcgtg cagtgtcacg ctcgttttta cccaggtact gaacagcgaa gttcaggcca
     2041 tcaaccagac caaagaagtt ggagttacga taggtagcaa cgccgccaac acgaccaacg
     2101 aagaagtcat cgctgtatgc agtatcacca ccaaattctg gcagcatatc ggtgtaaccc
     2161 agtgcatcat aaaccacacc gtagttacgg ccgtaatcga aagaaccaac gtcagcgtat
     2221 ttaagacccg cgaatgccag acgcgttttg ttaccagttt gagcgtcagc gccttcagag
     2281 ttgttaccct ggaagttata ttcccactga ccataaccgg tcagatcgga attgatttga
     2341 gtttcccctt taaaaccaag acgggcatag gtcatgtcgc cattgccacc gtaactgttt
     2401 tcaccgttac ccttggaaaa ataatgcaga ccaacagctt taccgtacag atctactttg
     2461 ttgccatctt tgttatagat ttctgcagcg tttgcagtac ctgctactaa cagagcaggg
     2521 acgatcactg ccagaatatt gcgcttcatc attatttatt accctcatgg ttttttttat
     2581 gacacctgcc actgccgtca ataagttctg tcaataaaaa tttacggaac tattgatgag
     2641 agtttggtgt ctttatgtgt ctgcaggcat ctttccattc aaactaacgt ttc
//
LOCUS       DAZKHF020000385         2665 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2201S31241
            SAMN43267800-rid25106413.denovo.387, whole genome shotgun sequence.
ACCESSION   DAZKHF020000385 DAZKHF020000000
VERSION     DAZKHF020000385.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267800
            Sequence Read Archive: SRR30307263
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2665)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2665)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 2665)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350581.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350581.2
            Genome Coverage       :: 151x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:21:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,652
            CDSs (total)                      :: 5,557
            Genes (coding)                    :: 5,320
            CDSs (with protein)               :: 5,320
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 237
            CDSs (without protein)            :: 237
            Pseudo Genes (ambiguous residues) :: 0 of 237
            Pseudo Genes (frameshifted)       :: 102 of 237
            Pseudo Genes (incomplete)         :: 130 of 237
            Pseudo Genes (internal stop)      :: 52 of 237
            Pseudo Genes (multiple problems)  :: 41 of 237
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2665
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267800-rid25106413.denovo.387"
                     /serotype="O 26:H11"
                     /isolate="2201S31241"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-01"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            <1..517
                     /gene="ybeT"
                     /locus_tag="ACHOSC_RS23515"
                     /old_locus_tag="pgaptmp_004703"
     CDS             <1..517
                     /gene="ybeT"
                     /locus_tag="ACHOSC_RS23515"
                     /old_locus_tag="pgaptmp_004703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HGU4550482.1"
                     /translation="VAAITCISHPKKTTLRDKAMVNYAFDYLSSPGSLPFTTAATELS
                     AIHGHSTSQYRLGEFYLHGSDGKPLDYTQARYWYEQSAEQENPRAQSKLGWIYLKGLG
                     VKPDTRKAILWYKEAAEQGYAHAQYTLGLIYRNGSGINVNHYESQKWLKLAAKQHYKN
                     AERLLAGLPAH"
     gene            complement(527..1954)
                     /gene="djlB"
                     /locus_tag="ACHOSC_RS23520"
                     /old_locus_tag="pgaptmp_004704"
     CDS             complement(527..1954)
                     /gene="djlB"
                     /locus_tag="ACHOSC_RS23520"
                     /old_locus_tag="pgaptmp_004704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="J domain-containing protein"
                     /protein_id="HGU4550483.1"
                     /translation="MKNCWKILEIEETTDIDIIRRAYLALLPSFHPETDPQGFKQLRQ
                     AYEEALRIAQSPAKSVWQPEEYEVAEHEILLAFRALLASDSERFLPSAWQRFIQQLNY
                     CSMEEIDELRWSLCTIAMNTAHLSFECVVLLAERLRWLQEENVGEIDEEELESFLYAI
                     AKGNVFNFQTILHLPVAVQNDTIDFYQMFARIWSSHPEWLTLYLAQHRAVIIPDDAKL
                     HRNLLRWYSAGRLGIPELLDYARSWREAESDNEDARYYEYAQRVYCGEGESLLAELCD
                     YWREYPSTQADALILQWCRQHRVDYYPLVVMMIEARELVNDQGKPLLYIPGDSARTRF
                     HLYEILSDEKLSALGRSLVEMVLHKGRKPRISLTRDTEHPLWPLYLVAKQLVQANQPT
                     EESLMPIVSRLDAEDRCPLEALIIRRLLIQAANFTEKQTVEPEPQPQPMPVDDGGPGC
                     LGIIKIIFYIFIFAGLIGKILHLFG"
     gene            complement(1951..2658)
                     /locus_tag="ACHOSC_RS23525"
                     /old_locus_tag="pgaptmp_004705"
     CDS             complement(1951..2658)
                     /locus_tag="ACHOSC_RS23525"
                     /old_locus_tag="pgaptmp_004705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1266 domain-containing protein"
                     /protein_id="HGU4550484.1"
                     /translation="MDMESQKILFALSTPMEIRNECCLPSHSSPKMYLGTCFFDLSSS
                     WGIDARDDLLRTIHRMIDNGHAARLAGFYHRWFRYSPCEWRDYLAELNEQGQAYAQFV
                     ASTAECCGEGGIKAWDYVRMGFLSRMGVLNNWLSEEESLWIQSRIHLRALRYYRNWRQ
                     YFAGYTFGRQYWQSPEDDHLQLLREFLARKEYDDSGNDMFYQLFASDDAYYPTLSWQP
                     LAYYSACPETLKDMSDL"
BASE COUNT          756 a          642 c          597 g          670 t
ORIGIN      
        1 agttgcagca attacctgta ttagccaccc caagaaaaca acgttgcgtg ataaagccat
       61 ggtgaattat gcctttgatt atttaagctc accgggcagt ctaccgttta ccacggcggc
      121 cacggagctt tccgcgattc atggtcactc aacgtcgcaa tatcgccttg gagaatttta
      181 tcttcatggt agcgacggta aaccactgga ttatacacag gcgagatact ggtatgagca
      241 atcagcagaa caggaaaatc cgcgcgcaca aagcaaactg gggtggatct acctcaaagg
      301 tctgggggtc aaacccgaca cccgtaaagc aattctctgg tataaggaag cagctgaaca
      361 agggtatgct catgctcaat atactttagg tttgatctac agaaatggct caggtattaa
      421 tgttaaccat tatgaatctc aaaaatggtt aaaactggcc gccaaacaac attacaaaaa
      481 tgcggaaaga ttacttgccg ggcttcccgc acattaacgt cgactgtcaa ccgaacagat
      541 ggagtatttt ccctatcaaa ccagcaaaga taaaaatata gaaaataatt ttaatgatgc
      601 ccagacagcc tggcccacca tcgtcaacgg gcattggctg cggttgcggc tccggttcaa
      661 cagtttgctt ctcggtaaaa ttcgccgcct gaattaataa tcgacgaata attaatgctt
      721 ccagtggaca acgatcttct gcatcaaggc ggctcacaat cggcattaat gattcttcgg
      781 ttggttggtt cgcctgcact aattgtttgg caactaaata taatggccat aagggatgtt
      841 ctgtatcacg cgtgagtgag atccgcggct tacgtccttt gtgcaaaacc atctcgacca
      901 gtgaacgccc cagagcagag agtttttcat cgctgagtat ttcgtataaa tgaaaacgcg
      961 tacgggcgct gtcgccgggg atataaagta gcggttttcc ctgatcgtta accagttcac
     1021 gagcttcaat catcatcacc actaacgggt aataatcgac ccgatgctga cggcaccatt
     1081 gcaatattaa agcatccgcc tgggtggagg gatactcgcg ccagtagtca caaagttctg
     1141 ccagcaagct ttcgccttct ccacaataga cgcgttgtgc gtattcataa taacgcgcat
     1201 cttcattatc agattcagct tcccgccacg accgggcgta atccaggagt tcgggaattc
     1261 ccaggcgacc tgcgctatac cagcggagta aatttctgtg cagttttgca tcatcgggga
     1321 taatcactgc gcgatgttgc gctaaataca atgtcagcca ttctggatgc gatgaccaaa
     1381 tccgagcgaa catttggtaa aaatcgatag tgtcattttg cacggcaacg ggcagatgca
     1441 gaatggtctg gaagttaaaa acattcccct tcgcaatggc atataaaaag gattccagtt
     1501 cttcttcgtc tatttccccg acgttttcct cttgcaacca ccgcaatctt tctgctaata
     1561 acaccacgca ctcgaaggat aaatgggcag tgttcatggc gattgtgcac agcgaccagc
     1621 gtaattcatc aatctcttcc atcgagcaat aatttaattg ctgaatgaat cgctgccagg
     1681 cggagggcag aaaacgttcg ctatcagagg caagtaacgc acgaaaggcg agcagaattt
     1741 catgttctgc tacctcatat tcttctggtt gccaaacaga tttagcaggc gactgcgcaa
     1801 tccgtagcgc ctcctcatac gcctgacgaa gttgtttaaa accctgcgga tcggtttctg
     1861 gatgaaagga cggtaacagc gccagataag cgcggcggat aatatcgatg tcagtcgttt
     1921 cctctatttc gagaatcttc cagcaatttt tcataggtcg ctcatatcct taagcgtttc
     1981 cgggcatgca gaatagtaag ccaatggttg ccaggacaag gtagggtaat acgcatcatc
     2041 actggcaaat aattgataaa acatatcatt gccggaatcg tcgtattctt tacgcgctaa
     2101 gaattcgcgt aataactgca gatgatcatc ttcgggagac tgccaatatt gccgaccaaa
     2161 ggtataaccg gcaaagtatt gccgccagtt acgataataa cgtagagccc gtagatgaat
     2221 gcgcgactgg atccatagac tttcttcttc gctcaaccag ttattaagta cgcccattcg
     2281 gcttaaaaaa cccatccgga cataatccca ggctttaatt cctccctcac cacagcattc
     2341 ggcggtgctg gcgacaaatt gcgcataagc ctgaccttgc tcatttagtt cagcaagata
     2401 gtcacgccat tcacatggcg aataacgaaa ccagcggtga taaaaccctg ccaaccgagc
     2461 ggcatgaccg ttatcgatca ttcggtgaat tgtgcgcagc aggtcatcac gggcatcaat
     2521 tccccaggag gaagaaaggt caaaaaagca tgtacctaaa tacattttgg gtgatgaatg
     2581 cgaaggtaaa cagcattcat tgcgtatttc catcggagtg gaaagtgcaa acaatatttt
     2641 ttgcgactcc atgtccatat aattt
//
LOCUS       DAZKHF020000386         2659 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2201S31241
            SAMN43267800-rid25106413.denovo.388, whole genome shotgun sequence.
ACCESSION   DAZKHF020000386 DAZKHF020000000
VERSION     DAZKHF020000386.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267800
            Sequence Read Archive: SRR30307263
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2659)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2659)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 2659)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350581.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350581.2
            Genome Coverage       :: 151x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:21:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,652
            CDSs (total)                      :: 5,557
            Genes (coding)                    :: 5,320
            CDSs (with protein)               :: 5,320
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 237
            CDSs (without protein)            :: 237
            Pseudo Genes (ambiguous residues) :: 0 of 237
            Pseudo Genes (frameshifted)       :: 102 of 237
            Pseudo Genes (incomplete)         :: 130 of 237
            Pseudo Genes (internal stop)      :: 52 of 237
            Pseudo Genes (multiple problems)  :: 41 of 237
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2659
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267800-rid25106413.denovo.388"
                     /serotype="O 26:H11"
                     /isolate="2201S31241"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-01"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            <1..179
                     /locus_tag="ACHOSC_RS23530"
                     /old_locus_tag="pgaptmp_004706"
     CDS             <1..179
                     /locus_tag="ACHOSC_RS23530"
                     /old_locus_tag="pgaptmp_004706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4550485.1"
                     /translation="KVAIRTIDRHAGEGYAKEHPELISAFMTTAAANFATLTEREIAE
                     AEQVTTINVKSAGE"
     gene            310..504
                     /locus_tag="ACHOSC_RS23535"
                     /old_locus_tag="pgaptmp_004707"
     CDS             310..504
                     /locus_tag="ACHOSC_RS23535"
                     /old_locus_tag="pgaptmp_004707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000391150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4550486.1"
                     /translation="MEEIKQSATVMIDHEILNELKKLQFDIVRRMNESGIYGRAATPT
                     IGHLARVLLRERLGIATSEA"
     gene            508..669
                     /locus_tag="ACHOSC_RS23540"
                     /old_locus_tag="pgaptmp_004708"
     CDS             508..669
                     /locus_tag="ACHOSC_RS23540"
                     /old_locus_tag="pgaptmp_004708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001018604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antitermination protein NusG"
                     /protein_id="HGU4550487.1"
                     /translation="MAITEREASIIKAIGEETRDTIAPLMEKIAELETRINKLSDEVE
                     GLISLLGEQ"
     gene            797..1285
                     /locus_tag="ACHOSC_RS23545"
                     /old_locus_tag="pgaptmp_004709"
     CDS             797..1285
                     /locus_tag="ACHOSC_RS23545"
                     /old_locus_tag="pgaptmp_004709"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001229488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase small subunit"
                     /protein_id="HGU4550488.1"
                     /translation="MALTTKKKRFADALSKGTNPTEAAIHAGYSEKTARVKGWQLSND
                     PEVKKYLKAVTSDVTFQVTKPKKVTKKVTEKVTPERASINTIEMMDNGLPDPIKAMGR
                     ILIDNMDTDPKLALDAAFKLAQFTHRKIGSIGKKEVKKLNAANVSSNRFPIPKPPTGL
                     KH"
     gene            1297..1458
                     /locus_tag="ACHOSC_RS23550"
                     /old_locus_tag="pgaptmp_004710"
     CDS             1297..1458
                     /locus_tag="ACHOSC_RS23550"
                     /old_locus_tag="pgaptmp_004710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000006074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4550489.1"
                     /translation="MSEYITDPIDVLTEIMNDKQQPTEIRLQAAAVLMPYFHETLTPC
                     YQEEEDNDF"
     gene            1448..2371
                     /locus_tag="ACHOSC_RS23555"
                     /old_locus_tag="pgaptmp_004711"
     CDS             1448..2371
                     /locus_tag="ACHOSC_RS23555"
                     /old_locus_tag="pgaptmp_004711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000562896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage major capsid protein"
                     /protein_id="HGU4550490.1"
                     /translation="MISKNMKGVSAVRAASVLTSCKFSHADAIEMTKKNDPAVSKFLE
                     KSIVAGMTTDNMPSLVAEPIVRELGEYIFSQSVPGKLISSALNLPFGAKLQSISGKGS
                     KWLKEAEEIPVKEGEVNESALKLCKLGAIVMLNNELIKFSTPGTNSAIRDLITSESVK
                     EIDKKFLSSDQEVAGVSPEGVLNGADEAENFIELFKKHIANGNTLATSSLILPVENAL
                     QLTDAEFWQIELLRIPVIVSEQADRMMLIDAQKLIINVEATVITPTDEAVIKTESGTV
                     SLFQNDATAFRAITYCGWQKLDKAVTVLAKS"
BASE COUNT          857 a          583 c          634 g          585 t
ORIGIN      
        1 taaaggtggc aatcagaacg atagaccgcc acgcggggga aggatacgcg aaggagcatc
       61 ccgaactgat aagcgcattc atgaccacag cggcggcaaa ctttgccacg ctgacagaac
      121 gggagattgc cgaagcggaa caggtaacaa ccatcaacgt taaaagcgct ggagaataac
      181 cgaagaagtg agcacatagc cggagcaatc cggctttttt tcgatctcat atatcttcaa
      241 agatggttaa ttaatgataa tctatcgaaa atactggtta tgcaatgata agtatcagga
      301 gaaaacaaag tggaagagat aaagcaaagc gctaccgtga tgattgatca tgaaatcctt
      361 aacgagctta aaaagttgca gttcgatatt gtgcgccgga tgaatgaatc aggtatctac
      421 ggcagggcgg caacaccaac aatagggcat cttgcgcgcg tactgttacg cgaacgcctg
      481 ggcattgcaa caagtgaggc gtaaacaatg gctatcacag aacgcgaggc aagcatcatt
      541 aaagccattg gcgaggaaac aagagacacc atagcgccat tgatggaaaa gatcgcagag
      601 cttgagacac gaataaacaa gctgagtgat gaagttgaag gcctgatctc gttactcggt
      661 gagcaatgac agggggggag tgttccggcc ggaaagtcga tcgccctgga caccgccccc
      721 agtctcattc atagaaaatt ttgcttttca aaaaagttac acgaaaagtt acaccccctc
      781 aaaacgaggt ttttgcatgg cattaacgac caaaaaaaag cggtttgctg atgcgttatc
      841 aaagggcaca aatccaacgg aagccgcgat acatgcggga tacagcgaaa agactgcaag
      901 ggttaaaggc tggcagctat ccaatgatcc ggaggtgaaa aagtatctta aggctgtaac
      961 ttctgatgta acttttcagg ttacaaaacc gaaaaaagtt acaaagaaag ttaccgaaaa
     1021 agttacaccg gaacgggcat caattaacac cattgagatg atggataacg gcctacccga
     1081 ccccatcaaa gcaatgggca ggatactgat tgacaacatg gacacagatc caaaactggc
     1141 tttagatgcc gcgtttaagc tggcccagtt cacccaccgc aagatcggca gcattgggaa
     1201 aaaagaggtt aaaaaactca atgccgctaa tgtctcctca aatcggttcc ccatccctaa
     1261 gccaccaact ggcttaaaac actgaggtag cgaactatga gcgaatacat cacagaccct
     1321 attgatgtac tcacggaaat catgaacgac aagcagcagc caaccgaaat acggttacag
     1381 gctgccgctg ttctgatgcc atattttcac gaaaccctaa ccccatgtta ccaggaggaa
     1441 gaagataatg atttctaaaa acatgaaagg cgtgtcggca gttcgcgccg ccagcgtgct
     1501 tacatcgtgt aaattcagcc atgcagacgc aattgaaatg acgaaaaaaa atgatccggc
     1561 agtatcgaaa tttcttgaaa aatccattgt tgccggaatg actacggata atatgccgtc
     1621 gctggtggca gagccgatag taagagagct tggcgagtat attttttcgc agtctgtacc
     1681 aggcaagcta atcagcagcg cgctgaatct tccgtttggc gcaaaattgc agtcaatatc
     1741 aggcaagggt agtaaatggc ttaaagaagc cgaagaaatc cctgtcaaag agggagaagt
     1801 aaacgaaagt gcgctaaaac tgtgcaaact tggcgctatt gtgatgctga ataacgagct
     1861 aatcaaattt agcacccctg gtaccaattc agcgatcaga gacttgatca catcggaatc
     1921 ggtgaaagaa atagataaaa aatttctttc cagtgaccag gaggtggcgg gcgtatcccc
     1981 tgagggcgtg cttaatggtg ctgatgaggc cgaaaacttc atagagcttt tcaaaaagca
     2041 catagcgaac ggtaacacgc ttgccacgtc gtcgctaatc ttgcccgtag aaaacgcctt
     2101 acagcttact gatgctgagt tctggcagat agagcttttg agaataccgg taattgtgag
     2161 cgaacaggcg gatcgcatga tgctgataga cgctcaaaag ctgattatta acgttgaagc
     2221 cacggttata accccaactg atgaggcggt aataaaaacc gaatcaggga cggttagcct
     2281 tttccagaat gacgctacag cgttcagggc aatcacctat tgcggatggc aaaaactcga
     2341 taaggccgtc accgttctgg cgaaaagctg atttttccgg catcaatccc gatatggtga
     2401 tccccatatc gggataatta cgaaattttc gcagtttaat taatggtcag attaatcgct
     2461 aacccgctga tattttcgga aacctcaatt tgaggaagtc ggaagcgtca cccttcaaat
     2521 cctgaatggt cataatgacc acgcagacca tcaaggcaac cataacgaac atcgttacgg
     2581 ttggagcttt taccacagga aaactcgcat ttaaattgta cactttttca gcaaatgatt
     2641 ttagttatac gtttagtta
//
LOCUS       DAZKHF020000387         2657 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2201S31241
            SAMN43267800-rid25106413.denovo.389, whole genome shotgun sequence.
ACCESSION   DAZKHF020000387 DAZKHF020000000
VERSION     DAZKHF020000387.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267800
            Sequence Read Archive: SRR30307263
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2657)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2657)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 2657)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350581.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350581.2
            Genome Coverage       :: 151x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:21:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,652
            CDSs (total)                      :: 5,557
            Genes (coding)                    :: 5,320
            CDSs (with protein)               :: 5,320
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 237
            CDSs (without protein)            :: 237
            Pseudo Genes (ambiguous residues) :: 0 of 237
            Pseudo Genes (frameshifted)       :: 102 of 237
            Pseudo Genes (incomplete)         :: 130 of 237
            Pseudo Genes (internal stop)      :: 52 of 237
            Pseudo Genes (multiple problems)  :: 41 of 237
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2657
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267800-rid25106413.denovo.389"
                     /serotype="O 26:H11"
                     /isolate="2201S31241"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-01"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            33..710
                     /gene="tnpA"
                     /locus_tag="ACHOSC_RS23560"
                     /old_locus_tag="pgaptmp_004712"
     CDS             33..710
                     /gene="tnpA"
                     /locus_tag="ACHOSC_RS23560"
                     /old_locus_tag="pgaptmp_004712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001743492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS66-like element accessory protein TnpA"
                     /protein_id="HGU4550491.1"
                     /translation="MSIIFNGHYRMKHRTWITEALRLHFEEHLPRVVAGRRLGVPKST
                     VCSMFVRFRRAGLSWPLPAGMSEQELDACLYGQFSTVPVVRPESTVISEAPVVKKRPR
                     RPNFPYEFKIALVEQSLQPGACVAQIARENGINDNLLFNWRHQYRKGGLLPSGKNMPA
                     LLPVTLTPEPDNKIPAPAQEPEQINTPSDSLCCELVLPAGTLRLKGKLTPALLQTLIR
                     EIKGSSH"
     gene            710..1057
                     /gene="tnpB"
                     /locus_tag="ACHOSC_RS23565"
                     /old_locus_tag="pgaptmp_004713"
     CDS             710..1057
                     /gene="tnpB"
                     /locus_tag="ACHOSC_RS23565"
                     /old_locus_tag="pgaptmp_004713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858152.1"
                     /note="TnpB, as the term is used for proteins encoded by
                     IS66 family insertion elements, is considered an accessory
                     protein, since TnpC, encoded by a neighboring gene, is a
                     DDE family transposase; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family insertion sequence element accessory
                     protein TnpB"
                     /protein_id="HGU4550492.1"
                     /translation="MISLPAGSRIWLVAGITDMRNGFNGLASKVQNVLKDDPFSGHLF
                     IFRGRRGDQIKVLWADSDGLCLFTKRLERGRFVWPVTRDGKVHLTPAQLSMLLEGINW
                     KHPKRTERAGIRI"
     gene            1077..2648
                     /locus_tag="ACHOSC_RS23570"
                     /old_locus_tag="pgaptmp_004714"
     CDS             1077..2648
                     /locus_tag="ACHOSC_RS23570"
                     /old_locus_tag="pgaptmp_004714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012904571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS66-like element ISCro1 family transposase"
                     /protein_id="HGU4550493.1"
                     /translation="MDTSLAHENARLRALLQTQQDTIRQMAEYNRLLSQRVAAYASEI
                     NRLKALVAKLQRMQFGKSSEKLRAKTERQIQEAQERISALQEEMAETLGEQYDPVLPS
                     ALRQSSARKPLPASLPRETRVIRPEEECCPACGGELSSLGCDVSEQLELISSAFKVIE
                     TQRPKQACCRCDHIVQAPVPSKPIARSYAGAGLLAHVVTGKYADHLPLYRQSEIYRRQ
                     GVELSRATLGRWTGAVAELLEPLYDVLRQYVLMPGKVHADDIPVPVQEPGSGKTRTAR
                     LWVYVRDDRNAGSQMPPAVWFAYSPDRKGIHPQNHLAGYSGVLQADAYGGYRALYESG
                     RITEAACMAHARRKIHDVHARAPTYITTEALQRIGELYAIEAEVRGCSAEQRLAARKA
                     RAAPLMQSLYDWIQQQMKTLSRHSDTAKAFAYLLKQWDALNVYCSNGWVEIDNNIAEN
                     ALRGVAVGRKNWMFAGSDSGGEHAAVLYSLIGTCRLNNVEPEKWLRYVIEHIQDWPAN
                     RVRDLLPWKVDLSSQ"
BASE COUNT          639 a          728 c          739 g          551 t
ORIGIN      
        1 tctggtctgt ttcctccggc tctacaaaaa taatgtccat catttttaat ggacactatc
       61 gtatgaaaca ccggacctgg atcactgaag ctttacgtct tcactttgaa gaacatttac
      121 cccgggttgt ggccgggcgt cgcctgggtg taccaaaatc aacagtttgt agtatgttcg
      181 tgcgctttcg gagagctggc ctttcgtggc ctttgcccgc aggcatgtcg gagcaggaac
      241 ttgatgcctg cctttacgga caattttcca cggtaccagt cgtacgtcct gaaagcaccg
      301 ttatatccga agcccccgtg gtaaaaaaac gtccccggcg gcccaacttc ccttatgagt
      361 ttaaaatcgc cttagtggag cagtcactgc agcccggagc ctgtgtggcg cagatcgccc
      421 gggaaaacgg aatcaacgat aacctgctct tcaactggcg ccatcaatac cggaaaggtg
      481 gcctgctgcc ttccggaaaa aatatgccgg cactgcttcc cgtgacgtta acgccggagc
      541 cggataataa aatcccggcc cccgcacagg aaccagagca gataaataca ccgtccgaca
      601 gtctgtgttg tgagctggtt ctgccggccg gaactctcag gcttaaaggt aaactgacgc
      661 cggcgttatt acagacactt atccgcgaaa taaaagggag cagccactga tgatatctct
      721 ccctgcaggt tcgcgtatct ggctggttgc aggtatcacc gatatgcgaa atggctttaa
      781 cggcctggca tcaaaagttc agaacgtcct gaaggatgac ccgttctccg gacacctgtt
      841 catcttccgc ggacgccggg gtgaccagat aaaagtgttg tgggctgaca gtgacggact
      901 gtgcctcttc accaaacgcc tggagcgggg ccgcttcgtc tggccagtca cccgtgacgg
      961 caaggtgcac cttactccgg ctcagttatc catgcttctt gaaggtatca actggaagca
     1021 cccgaaacga acggaacgcg ctggaatccg catataaccc gttgtaaagt gaggatatgg
     1081 acacctcact tgctcatgag aacgcccgcc tgcgggcact gttgcagacg caacaggaca
     1141 ccatccgcca gatggctgaa tacaaccgcc tgctctcaca gcgggtggcg gcttatgctt
     1201 ccgaaatcaa ccggctgaag gcgctggttg cgaaactgca acgtatgcag ttcggtaaaa
     1261 gctcagaaaa acttcgtgca aaaaccgaac ggcagataca ggaagcacag gagcgaatca
     1321 gcgcacttca ggaagaaatg gcggaaacgc tgggtgagca atatgacccg gtactgccat
     1381 ccgccctgcg ccagtcttca gcccgtaaac cgttaccggc ctcacttccc cgtgaaaccc
     1441 gggttatccg gccggaagag gaatgctgtc ctgcctgtgg tggtgaactc agttctctgg
     1501 gatgtgatgt gtcagagcaa ctggagctta tcagcagcgc ctttaaggtt atcgaaacac
     1561 aacgtccgaa acaggcctgt tgccggtgcg accatatcgt gcaggcacca gtaccttcaa
     1621 aacccattgc acgcagttat gccggagcgg ggcttctggc ccatgttgtc accgggaaat
     1681 atgcagacca tctgccgtta taccgccagt cagaaatata ccgtcgtcag ggagtggagc
     1741 tgagccgtgc cacactgggg cgctggacag gtgctgttgc tgaactgctg gagccgctgt
     1801 atgacgtcct gcgccagtat gtgctgatgc ccggtaaagt ccatgctgat gatatccccg
     1861 tcccggtcca ggagccgggc agcggtaaaa cccggacagc ccggctgtgg gtctacgtcc
     1921 gtgatgaccg taacgccggt tcacagatgc ccccggcggt ctggttcgcg tacagtccgg
     1981 accggaaagg tatccatcca caaaatcacc tggccggtta cagcggtgtg cttcaggccg
     2041 atgcttacgg tggttaccgg gcgttatacg aatccggcag aataacggaa gccgcgtgta
     2101 tggctcatgc ccggagaaaa atccacgatg tgcatgcaag agcgcccacc tacatcacca
     2161 cggaagccct gcagcgtatc ggtgaactgt atgccatcga ggcagaggtc cggggctgtt
     2221 cagcagaaca gcgtctggcg gcaagaaaag ccagagccgc gccactgatg cagtcactgt
     2281 atgactggat acagcaacag atgaaaacac tgtcgcgtca ctcagatacg gcaaaagcgt
     2341 tcgcatacct gctgaaacag tgggatgcac tgaacgtgta ctgcagtaat ggctgggtgg
     2401 aaatcgacaa caacatcgca gagaacgcct tacggggagt ggccgtaggc cggaaaaact
     2461 ggatgttcgc gggttccgac agcggtggtg aacatgcggc ggtgttgtac tcgctgatcg
     2521 gcacatgccg tctgaacaat gtggagccag aaaagtggct gcgttacgtc attgaacata
     2581 tccaggactg gccggcaaac cgggtacgcg atctgttgcc ctggaaagtt gatctgagct
     2641 ctcagtaaat atcaata
//
LOCUS       DAZKHF020000388         2655 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2201S31241
            SAMN43267800-rid25106413.denovo.390, whole genome shotgun sequence.
ACCESSION   DAZKHF020000388 DAZKHF020000000
VERSION     DAZKHF020000388.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267800
            Sequence Read Archive: SRR30307263
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2655)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2655)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 2655)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350581.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350581.2
            Genome Coverage       :: 151x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:21:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,652
            CDSs (total)                      :: 5,557
            Genes (coding)                    :: 5,320
            CDSs (with protein)               :: 5,320
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 237
            CDSs (without protein)            :: 237
            Pseudo Genes (ambiguous residues) :: 0 of 237
            Pseudo Genes (frameshifted)       :: 102 of 237
            Pseudo Genes (incomplete)         :: 130 of 237
            Pseudo Genes (internal stop)      :: 52 of 237
            Pseudo Genes (multiple problems)  :: 41 of 237
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2655
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267800-rid25106413.denovo.390"
                     /serotype="O 26:H11"
                     /isolate="2201S31241"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-01"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            164..850
                     /locus_tag="ACHOSC_RS23575"
                     /old_locus_tag="pgaptmp_004715"
     CDS             164..850
                     /locus_tag="ACHOSC_RS23575"
                     /old_locus_tag="pgaptmp_004715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016232851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2726 domain-containing protein"
                     /protein_id="HGU4550494.1"
                     /translation="MSFMAFVTCLTIAFIAGFAVARKLTLRKVASSVIIAKSQPVPSS
                     ATSPAAKASTRSYIPQKTYRDSRWQERDELFQGMVAEGILLERKEWLITRCEKMYFDN
                     LEKWFGHYCHIHCQVSLGRLINFPEQPEFSEEERKRFFAIYNGMAMDYVLVSKKNNKI
                     VCVIELDDPSHERADRIIRDRWLDTIMTLARVPFVRASVTKLEEIPPVWETRDKIRPL
                     VAATKSEPVH"
     gene            complement(941..1077)
                     /locus_tag="ACHOSC_RS23580"
                     /old_locus_tag="pgaptmp_004716"
     ncRNA           complement(941..1077)
                     /ncRNA_class="other"
                     /locus_tag="ACHOSC_RS23580"
                     /old_locus_tag="pgaptmp_004716"
                     /product="RtT sRNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00391"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="rtT sRNA, processed from tyrT transcript; Derived
                     by automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF00391"
     gene            complement(1121..1257)
                     /locus_tag="ACHOSC_RS23585"
                     /old_locus_tag="pgaptmp_004717"
     ncRNA           complement(1121..1257)
                     /ncRNA_class="other"
                     /locus_tag="ACHOSC_RS23585"
                     /old_locus_tag="pgaptmp_004717"
                     /product="RtT sRNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00391"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="rtT sRNA, processed from tyrT transcript; Derived
                     by automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF00391"
     gene            1476..>2655
                     /locus_tag="ACHOSC_RS23590"
                     /old_locus_tag="pgaptmp_004718"
     CDS             1476..>2655
                     /locus_tag="ACHOSC_RS23590"
                     /old_locus_tag="pgaptmp_004718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HGU4550495.1"
                     /translation="MAVLTDTKARHIKPDDKPLPHGGITGLTLHPSSVKGRGKWVFRY
                     VSPVTQKRRNAGLGTYPEVSIAEAARTARIMREQLAAGDDPLEIKKAESEKVAIPTFA
                     DAARRVHAELSPGWENPKHVRQWLSTLENYAFPQLGAKTLDSITAADVAETLRPVWLT
                     LSETASRVKQRIHVVMQWGWAHGFCVANPVDVVDHLLPQQTRGRDEHQPAMPWRQLPL
                     FVATSVYTDEPYNVTRALLLMVILTATRSGEARGMRWAEIDFHKRVWTIPAERMKARI
                     QHRVPLSRQAIYILENIRGLHDELVFPSPRKQQILSDMVLTSFLRKKKAVSDIPGRVA
                     TAHGFRSTFRDWCSEQGYSRDLAERALAHTLKNKVEAAYHRTDLLEQRVPMMQAWADY
                     V"
BASE COUNT          692 a          571 c          693 g          699 t
ORIGIN      
        1 tttttttgtg gaaggcaagc cgcaatggct aaatagggat ggttgtaata atcaaaacat
       61 gatactaact atatattgat cgttgcattg ttggcatgta acacgctgca gcgagtaaca
      121 ttggtaatga aatataatca acatggatag gtgaaaatta attatgagct ttatggcttt
      181 tgtaacttgt ttgacaattg catttatcgc cggttttgct gttgccagaa aattgacact
      241 ccgaaaagtt gcctcatcgg taatcatcgc taaatcgcaa cccgtccctt catctgcaac
      301 ttcccctgcc gctaaggcgt cgacaaggag ctatataccg cagaaaacct atcgtgactc
      361 tcgttggcaa gagcgagatg agttatttca gggaatggtt gccgaaggta ttttattgga
      421 aagaaaagag tggttaatca cgcgttgtga aaaaatgtat ttcgataatc tggaaaaatg
      481 gtttggacat tattgtcata tccattgcca ggtctctctg gggcgcttga tcaactttcc
      541 agaacaacct gagtttagcg aagaagaaag aaagcgtttc tttgcaattt ataatggcat
      601 ggcaatggat tatgttctgg tttcaaagaa gaacaataaa atagtctgtg ttattgagct
      661 ggatgacccg agtcatgaaa gggcggatcg cattattcgt gaccgttggt tggatacaat
      721 tatgactctt gcacgggtgc cttttgtacg agcttctgtc actaaattgg aggaaattcc
      781 tccggtctgg gagactcgtg acaaaattcg cccattagtc gcggcgacaa agagtgaacc
      841 ggtgcattaa ctaagcatcc cggtcggggc ttctcatccc cccggtgtgt gcaatatacg
      901 aaaaaaagcc cgtaccttcg tacgagctct tctttaaaga tggcggtgag ggggggattg
      961 actcgcttcg ctcgccctgc gggcagcccg ctcactgcgt tcacggtctg tccaactggc
     1021 tgtcgccagt tgtcgaaccc cggtcggggc ttctcatccc cccggtgtgt gcaatatacg
     1081 aaaaaaagcc cgtactttcg tacgagctct tctttaaata tggcggtgag ggggggattg
     1141 actcgcttcg ctcgccctgc gggcagcccg ctcactgcgt tcacggtctg tccaactggc
     1201 tgttgccagt tgtcgaaccc cggtcggggc ttctcatccc cccggtgtgt gaaatatacg
     1261 aaaaaaaagc ccgtactttc gtacgagctc ttctttaaat atggcggtga gggggaccgc
     1321 ctgaaataaa tctgacttaa tccaggggtg ttatataatt tcattataaa caatgtatta
     1381 cactttttgt tgtccgatat tatctaatgt attcttagtg aagcgagaga ctaatggcat
     1441 actggatggc ataccagagt gacgggaatt agcgtatggc agtattgacg gatacgaaag
     1501 caagacatat caaacctgat gacaaaccat tgccccatgg gggaattacc ggactgaccc
     1561 ttcatccttc ttcagtaaag gggcggggga aatgggtttt tcgttatgta agtccggtga
     1621 cacaaaaaag acgtaatgct ggattgggaa cttacccaga ggtcagtatt gctgaagctg
     1681 cacgtactgc ccggataatg cgagagcaac ttgctgcagg tgatgatcct ctggagatta
     1741 aaaaggctga atctgagaaa gtcgctatcc caacatttgc cgatgcagcc aggcgtgtac
     1801 atgcagaact gtctcctgga tgggaaaatc caaagcatgt aaggcagtgg ttatcgacgc
     1861 ttgagaatta cgcgtttcct caactgggag caaaaacgct ggattcgatt acggctgcgg
     1921 acgtggcaga aacactgcgt ccagtctggt taaccttgtc agaaacggca agccgggtta
     1981 aacagcgcat tcatgttgtt atgcagtggg gatgggcgca cggtttttgt gtagcaaatc
     2041 ctgttgatgt ggttgaccat ttgcttcctc agcagacaag aggacgtgat gaacaccaac
     2101 ccgcaatgcc ctggaggcag ttaccgcttt ttgtggcgac cagtgtgtat accgatgaac
     2161 cttataatgt tacccgcgca ctgttattaa tggtgatact tacagcaact cgctcgggcg
     2221 aagccagggg aatgcgctgg gctgaaattg attttcataa gcgggtatgg actatacctg
     2281 cagaaagaat gaaagccagg atacagcatc gtgttccttt atcccggcag gctatttaca
     2341 ttctggaaaa tatacgtggc ctgcatgatg aactggtgtt cccttcaccc agaaagcagc
     2401 agatcctttc cgatatggtg ttgacaagtt ttctgcgtaa aaagaaagcc gtcagtgaca
     2461 ttccggggcg agttgccacg gcacatggtt ttcgctcaac attcagggac tggtgtagcg
     2521 aacaggggta ttcgcgggat ctggcggaaa gggcgctcgc tcatacgctg aaaaataagg
     2581 ttgaggcggc atatcatcgt actgatctac tggagcagcg tgtaccgatg atgcaggcat
     2641 gggcggatta tgtga
//
LOCUS       DAZKHF020000389         2653 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2201S31241
            SAMN43267800-rid25106413.denovo.391, whole genome shotgun sequence.
ACCESSION   DAZKHF020000389 DAZKHF020000000
VERSION     DAZKHF020000389.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267800
            Sequence Read Archive: SRR30307263
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2653)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2653)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 2653)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350581.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350581.2
            Genome Coverage       :: 151x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:21:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,652
            CDSs (total)                      :: 5,557
            Genes (coding)                    :: 5,320
            CDSs (with protein)               :: 5,320
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 237
            CDSs (without protein)            :: 237
            Pseudo Genes (ambiguous residues) :: 0 of 237
            Pseudo Genes (frameshifted)       :: 102 of 237
            Pseudo Genes (incomplete)         :: 130 of 237
            Pseudo Genes (internal stop)      :: 52 of 237
            Pseudo Genes (multiple problems)  :: 41 of 237
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2653
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267800-rid25106413.denovo.391"
                     /serotype="O 26:H11"
                     /isolate="2201S31241"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-01"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            <1..517
                     /locus_tag="ACHOSC_RS23595"
                     /old_locus_tag="pgaptmp_004719"
     CDS             <1..517
                     /locus_tag="ACHOSC_RS23595"
                     /old_locus_tag="pgaptmp_004719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="lysozyme"
                     /protein_id="HGU4550496.1"
                     /translation="YGLSAAVLALIAAGAPAPEILDQFLDEKEGNHTTAYRDGAGIWT
                     ICRGATRVDGKPVLPGMKLSKEKCDQVNAIERDKALAWVEKNIKVPLTEPQKAGIASF
                     CPYNIGPGKCFPSTFYRRINAGDRRGACEAIRWWIKDGGRDCRIRSNNCYGQVSRRDQ
                     ESALACWGIDR"
     gene            810..941
                     /locus_tag="ACHOSC_RS23600"
                     /old_locus_tag="pgaptmp_004720"
     CDS             810..941
                     /locus_tag="ACHOSC_RS23600"
                     /old_locus_tag="pgaptmp_004720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001280968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4550497.1"
                     /translation="MSDKLITPAKVLCVIVGISFSLMLVALFLSLAWVMLSSSGLLG"
     gene            905..1060
                     /locus_tag="ACHOSC_RS23605"
                     /old_locus_tag="pgaptmp_004721"
     CDS             905..1060
                     /locus_tag="ACHOSC_RS23605"
                     /old_locus_tag="pgaptmp_004721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000788417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4550498.1"
                     /translation="MGDVVFVGAAGVTVTDDISRALAFAIKWVAVGIAVSPMLYGLAK
                     LVIALKS"
     gene            1072..1158
                     /locus_tag="ACHOSC_RS23610"
                     /old_locus_tag="pgaptmp_004722"
     CDS             1072..1158
                     /locus_tag="ACHOSC_RS23610"
                     /old_locus_tag="pgaptmp_004722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4550499.1"
                     /translation="MSAELIRAMAFAIRIVAIAVLVWAIRWW"
     gene            1380..1565
                     /locus_tag="ACHOSC_RS23615"
                     /old_locus_tag="pgaptmp_004723"
     CDS             1380..1565
                     /locus_tag="ACHOSC_RS23615"
                     /old_locus_tag="pgaptmp_004723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rz1 family lipoprotein"
                     /protein_id="HGU4550500.1"
                     /translation="MRELKMKLCVLMLPLVVSACGSTPPAPVPCVKPPAPPAWIMQPA
                     PDWQTPLNGIISSSENG"
     gene            1966..2292
                     /locus_tag="ACHOSC_RS23620"
                     /old_locus_tag="pgaptmp_004724"
     CDS             1966..2292
                     /locus_tag="ACHOSC_RS23620"
                     /old_locus_tag="pgaptmp_004724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000828066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015291 - secondary active transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB family protein"
                     /protein_id="HGU4550501.1"
                     /translation="MKRNLPLIILLSSLVMGCTQHKTDMPRQLVKALPQYPAYAAANY
                     IKGRVDVRFDIGADGTVTRIEFIRSEPHHLFDEQVVKAMAKWRFEKDRPCKGVKKTFI
                     FSPSAP"
     gene            complement(2424..2624)
                     /locus_tag="ACHOSC_RS23625"
                     /old_locus_tag="pgaptmp_004725"
     CDS             complement(2424..2624)
                     /locus_tag="ACHOSC_RS23625"
                     /old_locus_tag="pgaptmp_004725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000095744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlcI/YnfO family protein"
                     /protein_id="HGU4550502.1"
                     /translation="MSSKNRPRRTTTRNIRFPNHMIEQINIALEHKGSGNFSAWVIEA
                     CRRRLATDATHLRPASMTNNEK"
BASE COUNT          624 a          572 c          751 g          706 t
ORIGIN      
        1 atacggcctg tcggctgccg ttctggcgct gattgccgca ggtgcgcctg cgcctgaaat
       61 cctcgaccag tttctggatg aaaaggaagg taaccacacc acggcatacc gtgatggcgc
      121 gggtatctgg accatctgtc gcggtgccac ccgggtggat ggtaagcctg tcctcccggg
      181 catgaagttg tcgaaggaaa aatgcgacca ggttaacgcc attgaacgtg ataaggcgct
      241 ggcatgggtg gagaaaaaca tcaaagtgcc actgaccgaa ccccagaaag cggggatcgc
      301 gtcattctgt ccgtacaaca ttggtcccgg taagtgtttc ccgtcgacgt tttacagacg
      361 aattaatgca ggtgatcgca ggggagcgtg tgaggcgatt cgctggtgga ttaaggacgg
      421 tggcagagac tgccgtattc gttcaaacaa ctgctacggt caggtatccc gtcgtgacca
      481 ggagagcgcg ctggcgtgct ggggaatcga cagataagca gaatattttg ctgaaaaata
      541 aggcatggcc acgcgggcgg ataacacgaa atcctgcgaa ctggcgaaac gtaagtgaat
      601 aaaagtaaaa atcccgtttg ttggcagcaa gcggggtttt gtttttatgg cagtaagcta
      661 tgggaggctg ccttgattga ttttagcaaa ctgattaggg agttgcgact catgattagt
      721 caattaccaa actggaaatt tttgctggtc tggagcatcc cttttttatg ggtagtatcc
      781 cagttaattg tggcaattaa ggggtagcta tgtcagacaa actcataacg ccggcaaagg
      841 tcctctgtgt gattgtcggt atttcatttt cactaatgct ggttgctctt tttctgtccc
      901 tcgcctgggt gatgttgtct tcgtcggggc tgctggggtg acagtgactg atgacatcag
      961 cagagcgctg gcttttgcta ttaagtgggt ggctgttggt attgctgtgt ctccgatgct
     1021 atatgggctg gcaaaactgg tcattgcgct gaaatcgtga actttaaaaa gatgagtgct
     1081 gaacttattc gggcaatggc atttgccatt cgtattgtgg ccattgctgt tctggtctgg
     1141 gcaatccgtt ggtggtgaaa tgaaccgcgt tctgtgtgtg gtcatcattg ctctgctggt
     1201 ggcctgtggt gcgcttagtc tggggctgaa tcattaccgc gataacgcca tcacttacaa
     1261 agcgcagcgc gataaaaaag ccagtgaact gaagctggcg aacgcaacca ttactgatat
     1321 gcaggtgcgc cagcgcgatg ttgctgcgct cgatgcaaaa tactcgaggg aattagccga
     1381 tgcgagagct gaaaatgaaa ctctgcgtgc tgatgttgcc gctggtcgta agcgcctgcg
     1441 gatcaacgcc acctgctccg gtaccgtgcg tgaagccacc ggcacctccg gcgtggataa
     1501 tgcaaccggc ccccgactgg cagacaccgc tgaacgggat tatttcatcc tcagagaacg
     1561 gttgatgaca atgcagaagc agctggaagg ggcgcaggaa tatatccgta ctcagtgcct
     1621 gaaataagtt ttgttgatgc gccgtatcgt cgctgtattc cctcattaac agagaccgca
     1681 gcccgacagg gagactcctc tgcgcgagtg tgcggggata atcaaaaacg atacacaccg
     1741 gggtttaccg cgttaacgga gcgcggcgtt gtcccctcat agtcgccagt ccggtgcgat
     1801 ggtggaagaa acaggacgat gtgttacctc gcaagccctg ttatgtcatg tgtctgattt
     1861 gtgatttaag tcggataatt gtcgttgcca ttaagcagag gattgatgac cgacagggcg
     1921 gcattgttag aataagactt atttttatct gtgcggggaa tgaaaatgaa aagaaatctt
     1981 ccgttaatta ttttgttgtc ttctctggtt atgggctgta cgcaacataa aacagatatg
     2041 ccccgacagt tggttaaggc attaccacaa tatccggcct atgcagcggc aaattatata
     2101 aagggacggg ttgatgtgag gtttgatatt ggtgctgatg gtactgtcac ccgaattgag
     2161 tttattcgtt cagagccgca ccatctgttt gatgagcagg ttgtaaaggc gatggcaaaa
     2221 tggcgatttg agaaggacag gccgtgtaaa ggcgtgaaga aaacgtttat ctttagtcct
     2281 tctgcaccct gattatttca tcagaaatta attatcactc tgttgttatt ctgtacatcc
     2341 cggcagggta agtcttgttc cgtcggatat gaagatgaaa tattgtggga ggacagtggg
     2401 tacctgctcc tgtaaccgaa cgttcatttc tcgttatttg tcatgctggc cgggcgcaga
     2461 tgcgttgcat ctgttgccag ccttctcctg caggcttcaa taacccacgc tgaaaagtta
     2521 ccggaacctt tatgttcaag ggcgatattg atctgttcaa tcatgtgatt ggggaaacgg
     2581 atgttgcggg ttgttgttct gcggggccgg tttttcgatg acattttctt tcctctggtg
     2641 acaagctata tgg
//
LOCUS       DAZKHF020000390         2639 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2201S31241
            SAMN43267800-rid25106413.denovo.392, whole genome shotgun sequence.
ACCESSION   DAZKHF020000390 DAZKHF020000000
VERSION     DAZKHF020000390.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267800
            Sequence Read Archive: SRR30307263
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2639)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2639)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 2639)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350581.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350581.2
            Genome Coverage       :: 151x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:21:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,652
            CDSs (total)                      :: 5,557
            Genes (coding)                    :: 5,320
            CDSs (with protein)               :: 5,320
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 237
            CDSs (without protein)            :: 237
            Pseudo Genes (ambiguous residues) :: 0 of 237
            Pseudo Genes (frameshifted)       :: 102 of 237
            Pseudo Genes (incomplete)         :: 130 of 237
            Pseudo Genes (internal stop)      :: 52 of 237
            Pseudo Genes (multiple problems)  :: 41 of 237
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2639
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267800-rid25106413.denovo.392"
                     /serotype="O 26:H11"
                     /isolate="2201S31241"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-01"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            complement(<1..713)
                     /locus_tag="ACHOSC_RS23630"
                     /old_locus_tag="pgaptmp_004726"
     CDS             complement(<1..713)
                     /locus_tag="ACHOSC_RS23630"
                     /old_locus_tag="pgaptmp_004726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sialate O-acetylesterase"
                     /protein_id="HGU4550503.1"
                     /translation="MNAIISPDYYYILTIAGQSNAMAYGEGLPLPDREDAPHPRIKQL
                     ARFAHTHPGGPPCHFNDMIPLTHCPHDVQDMQGYHHPLATNHQTQYGTVGQALHIARK
                     LLPFIPDNAGVLIVPCCRGGSAFTAGSEGTYSERHGASHDACRWGTDTPLYQDLVSRT
                     RAALAKNPHNKFLGVCWMQGEFDLMTSDYASHPQHFNHMVEAFRRDLKQYHSQLNNIT
                     DAPWFCGDTTWYWKENFPHA"
     gene            complement(778..1884)
                     /gene="nanM"
                     /locus_tag="ACHOSC_RS23635"
                     /old_locus_tag="pgaptmp_004727"
     CDS             complement(778..1884)
                     /gene="nanM"
                     /locus_tag="ACHOSC_RS23635"
                     /old_locus_tag="pgaptmp_004727"
                     /EC_number="5.1.3.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418730.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylneuraminate epimerase"
                     /protein_id="HGU4550504.1"
                     /translation="MNKTITALAIMMASFAANASVLPETPVPFKSGTGAIDNDTVYIG
                     LGSAGTAWYKLDTQAKDKKWKALAAFPGGPRDQATSAFIDGNLYVFGGIGKNSEGLTQ
                     VFNDVHKYNPKTNSWVKLMSHAPMGMAGHVTFVHNGKAYVTGGVNQNIFNGYFEDLNE
                     AGKDSTAIDKINAHYFDKKAEDYFFNKFLLSFDPSTQQWSYAGESPWYGTAGAAVVNK
                     GDKTWLINGEAKPGLRTDAVFELDFTGNNLKWNKLAPVSSPDGVAGGFAGISNDSLIF
                     AGGAGFKGSRENYQNGKNYAHEGLKKSYSTDIHLWHNGKWDKSGELSQGRAYGVSLPW
                     NNSLLIIGGETAGGKAVTDSVLISVKDNKVTVQN"
     gene            complement(1904..2620)
                     /gene="nanC"
                     /locus_tag="ACHOSC_RS23640"
                     /old_locus_tag="pgaptmp_004728"
     CDS             complement(1904..2620)
                     /gene="nanC"
                     /locus_tag="ACHOSC_RS23640"
                     /old_locus_tag="pgaptmp_004728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313297.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylneuraminic acid outer membrane channel
                     NanC"
                     /protein_id="HGU4550505.1"
                     /translation="MKKAKILSGVLLLCFSSPLISQAATLDVRGGYRSGSHAYETRLK
                     VSEGWQNGWWASMESNTWNTIHDNKKENAALNDVQVDVNYAIKLDDQWTVRPGMLTHF
                     SSNGTRYGPYVKLSWDATKDLNFGIRYRYDWKAYRQQDLSGDMSRDNVHRWDGYVTYH
                     INSDFTFAWQTTLYSKQNDYRYANHKKWATENAFVLQYHMTPDITPYIEYDYLDRQGV
                     YNGRDNLSENSYRIGVSFKL"
BASE COUNT          674 a          621 c          562 g          782 t
ORIGIN      
        1 gcatgaggga aattttcttt ccagtaccag gtggtatcgc cgcaaaacca cggtgcgtca
       61 gtaatattat taagctgaga atggtattgt tttagatccc tacgaaaggc ttcaaccata
      121 tgattaaagt gttgagggtg tgacgcgtag tcactggtca ttaagtcaaa ttcgccttgc
      181 atccagcata cgccgaggaa tttgttatgc ggattttttg ccagtgcggc tcgcgttctg
      241 ctgactaaat cctggtatag cggagtatcc gttccccaac gacaagcatc atggctggct
      301 ccgtgccgtt ctgaatatgt cccttcgctg cccgcggtaa aagccgatcc gccacggcaa
      361 cacggaacga tgagaacccc tgcattatca ggaataaagg gcagtaattt ccgtgctata
      421 tgcagtgcct ggccaacggt gccgtactgt gtttgatgat tcgttgccag aggatggtga
      481 taaccctgca tatcctgaac atcgtgtggg cagtgagtca gtggaatcat gtcgttaaag
      541 tgacatggcg ggcctccggg atgcgtatgc gcaaatctcg ctaattgttt gattctggga
      601 tgaggcgcat cttccctgtc cggtaatggc agtccttcgc catacgccat ggcattagac
      661 tgaccagcaa tggtaagaat atagtaataa tcgggcgata ttattgcgtt catataaatt
      721 tcgcgtaatt gaaatgacaa attgatagca gggccccttt accggggccc tgaagcgtta
      781 gttttgtact gtaactttat tatccttcac agagatcaaa actgaatccg tcaccgcttt
      841 gccgcctgca gtttcaccgc caataatcaa tagactatta ttccaaggca atgatactcc
      901 gtaggcccga ccttgcgata attcacccga tttatcccat ttcccgttat gccaaagatg
      961 aatatcagtg ctatatgatt ttttcaggcc ttcatgcgca tagttcttac cgttctggta
     1021 attttctcgt gaacctttga atccggcccc tccggcaaat ataagagaat cattgcttat
     1081 ccccgcaaaa ccgccagcta cgccatctgg tgatgagacg ggagcaagct tattccattt
     1141 taaattatta ccggtgaaat caagttcaaa tacggcatcc gttcgcaatc ctggtttggc
     1201 ttcgccatta ataagccagg ttttatcacc tttattcaca accgccgcac cagccgttcc
     1261 gtaccagggc gattcgccag cgtaactcca ttgctgtgtt gagggatcaa aagacaacag
     1321 aaacttattg aaaaaataat cttctgcttt tttgtcaaaa tagtgagcgt tgattttatc
     1381 tatagcggtt gaatcttttc cagcctcgtt gagatcttca aaatagccat tgaagatatt
     1441 ctggttaaca ccgccagtaa cataagcctt gccgttgtgt acaaaagtta catgacccgc
     1501 catgcccatc ggtgcgtgcg acatcaattt aacccaacta ttggttttgg ggttgtattt
     1561 gtgtacgtca ttaaatacct gagtcaagcc ctcgctgttt ttgccaatgc cgccaaacac
     1621 atacagattg ccatcaataa atgccgaggt tgcttgatct cttggtccac cagggaatgc
     1681 agctaacgct ttccattttt tatctttggc ctgtgtatcc agcttgtacc atgccgtacc
     1741 tgcgctacct aaaccaatgt agacagtgtc gttatcaatt gctccggtac cacttttaaa
     1801 tggcacagga gtttccggta atacagacgc gtttgcggca aatgaagcca tcatgatagc
     1861 aagcgccgtt attgttttat tcattgtgac tgtctcctgt ctactacagt ttaaatgaca
     1921 caccaatgcg ataactgttt tccgataaat tatctctgcc gttgtaaaca ccctgacggt
     1981 caaggtagtc atattctatg tatggcgtaa tatccggcgt catatggtat tgtagaacaa
     2041 atgcattttc cgtcgcccat ttcttatggt ttgcatagcg ataatcgttc tgtttgctgt
     2101 atagcgtcgt ttgccatgcg aaggtgaaat cactattaat atggtaagtg acatatccat
     2161 cccaacgatg aacgttatca cgagacatat caccggataa gtcttgttgt cggtaagctt
     2221 tccagtcgta acgatagcga atgccaaaat taagatcttt tgtcgcgtcc caggacagtt
     2281 ttacgtaggg tccgtagcgt gtgccgttgc tgctaaaatg cgttaacatt cccgggcgca
     2341 ccgtccattg atcatcaagt ttaatcgcgt aattaacatc aacctgaaca tcattgagtg
     2401 cggcattttc ctttttatta tcatgaatgg tattccaggt attactttcc atacttgccc
     2461 accatccatt ttgccatccc tcactgactt tgagtcgagt ctcataggcg tggcttccac
     2521 tacgatatcc accacgtacg tccagtgtcg cagcttgaga aattaatggt gacgaaaagc
     2581 acagtaataa tacgccagaa agtattttag cctttttcat aaatttcact catttgtag
//
LOCUS       DAZKHF020000391         2620 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2201S31241
            SAMN43267800-rid25106413.denovo.393, whole genome shotgun sequence.
ACCESSION   DAZKHF020000391 DAZKHF020000000
VERSION     DAZKHF020000391.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267800
            Sequence Read Archive: SRR30307263
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2620)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2620)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 2620)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350581.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350581.2
            Genome Coverage       :: 151x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:21:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,652
            CDSs (total)                      :: 5,557
            Genes (coding)                    :: 5,320
            CDSs (with protein)               :: 5,320
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 237
            CDSs (without protein)            :: 237
            Pseudo Genes (ambiguous residues) :: 0 of 237
            Pseudo Genes (frameshifted)       :: 102 of 237
            Pseudo Genes (incomplete)         :: 130 of 237
            Pseudo Genes (internal stop)      :: 52 of 237
            Pseudo Genes (multiple problems)  :: 41 of 237
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2620
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267800-rid25106413.denovo.393"
                     /serotype="O 26:H11"
                     /isolate="2201S31241"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-01"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            62..280
                     /locus_tag="ACHOSC_RS23645"
                     /old_locus_tag="pgaptmp_004729"
     CDS             62..280
                     /locus_tag="ACHOSC_RS23645"
                     /old_locus_tag="pgaptmp_004729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000683609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4014 family protein"
                     /protein_id="HGU4550506.1"
                     /translation="MKHYLEKNYPRKSRTTEFLFFILFIVLMIPISPLLLVWIIGRTF
                     EPVIELYTDVTWESFSALHNKINPYKEN"
     gene            282..839
                     /locus_tag="ACHOSC_RS23650"
                     /old_locus_tag="pgaptmp_004730"
     CDS             282..839
                     /locus_tag="ACHOSC_RS23650"
                     /old_locus_tag="pgaptmp_004730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF551 domain-containing protein"
                     /protein_id="HGU4550507.1"
                     /translation="MSTITRERAEIKSYITGFLSDSAHDNKSSDSLLANVFRIALASL
                     EAEPIAMVVPDEMDLLTCHLDGVTKTYADGWNACRVAMLQAGNFRENKNSSTNNFREI
                     SETSTRSPITLDGWISCTERMPEKSQNVLISMNIDSEAGPLIYSARYLGGTFRRGGIA
                     VSPGNDLRQATHWMSLPEPPQEVNQ"
     gene            1130..1285
                     /locus_tag="ACHOSC_RS23655"
                     /old_locus_tag="pgaptmp_004731"
     CDS             1130..1285
                     /locus_tag="ACHOSC_RS23655"
                     /old_locus_tag="pgaptmp_004731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hok/Gef family protein"
                     /protein_id="HGU4550508.1"
                     /translation="MKQQKAMLIALIVICLTVIVTALVTRKDLCEVRIRTGQTEVAVF
                     VDYESEK"
     gene            1405..1749
                     /locus_tag="ACHOSC_RS23660"
                     /old_locus_tag="pgaptmp_004732"
     CDS             1405..1749
                     /locus_tag="ACHOSC_RS23660"
                     /old_locus_tag="pgaptmp_004732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YgiW/YdeI family stress tolerance OB fold
                     protein"
                     /protein_id="HGU4550509.1"
                     /translation="MKKVLIAALISGVSFGAFAQQGGFQGPEAERSTVAQAKELKDDA
                     WVILEGSIVKKVGDERYEFRDNSGTIVTDIDDSVWAGQNVSPKDKVRIEGEIDKDLSS
                     VEVDVKALKLLK"
     gene            1871..2143
                     /locus_tag="ACHOSC_RS23665"
                     /old_locus_tag="pgaptmp_004733"
     CDS             1871..2143
                     /locus_tag="ACHOSC_RS23665"
                     /old_locus_tag="pgaptmp_004733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4550510.1"
                     /translation="MTQHIKSHNSEADPEIKQGRRFRAPQYGWFHYLFCTIDEADMLQ
                     EAYLRRGVRVERSLNADRLTWTVSVYLPVRAHLPRTHACYRQRVWR"
     gene            2145..>2620
                     /locus_tag="ACHOSC_RS23670"
                     /old_locus_tag="pgaptmp_004734"
     CDS             2145..>2620
                     /locus_tag="ACHOSC_RS23670"
                     /old_locus_tag="pgaptmp_004734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310222.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4550511.1"
                     /translation="MRVLLRPVLVPELGLVVLKPGRESLPVFHRGRVLVEPEPKNMRA
                     LPSGAVPAVRQPLAEDKSLLPFFSDERVIRAAGGAGALSDWLLRHVKSCQWPHGDYHH
                     SETVIHSYGAGAMVLCWHCDNQLRDQTSESLEQLTQQNLTAWMIDVIRHVMNGTQER"
BASE COUNT          689 a          599 c          708 g          624 t
ORIGIN      
        1 taaccggagg gattcctgca ccctcagaac atcaggaggc cgcccgaaag ggcggtaaga
       61 aatgaaacat tatttagaaa aaaattaccc acgaaagagc agaacaacag agtttctgtt
      121 tttcattctg tttatagtgt tgatgatacc gatatccccg ctattactgg tctggataat
      181 tggaaggaca tttgaaccag ttattgagct atataccgat gtgacatggg aatcattcag
      241 cgcactgcac aataaaatta atccgtataa ggaaaactga tatgagcact attaccagag
      301 aacgcgcgga gattaaatca tacatcacag gcttcctgag cgactcggcg cacgataaca
      361 agtcttcaga cagcctgctg gctaatgtgt ttcgtatcgc gctggcatca ctggaagcag
      421 agccgatagc aatggtagtg cctgatgaaa tggatttgct tacctgccat ctcgacggtg
      481 taactaaaac atatgctgat ggctggaacg cctgccgcgt cgccatgctt caggccggaa
      541 actttcggga aaataagaat tcgtcaacca acaattttcg ggaaatctcg gaaacgtcaa
      601 ccagatctcc gataactctg gatggctgga taagctgtac tgagcgaatg cctgaaaaga
      661 gccagaacgt gcttatttcg atgaatatcg atagcgaggc tgggccatta atatattccg
      721 cacgctatct cggaggcacg ttccggcgcg gaggtatagc agttagtccg ggtaatgatc
      781 ttaggcaagc aacccactgg atgtcgctac cagaaccgcc gcaggaggtg aatcaatgac
      841 ctggcctgaa gcattcacaa cggtaggaat tgcgatggcg gtggcgctgg tggtgtattc
      901 gatttgccgc tggggataaa aacggtttgc gggaaaagga tagttaagta gaattgcagc
      961 gggtgcttga ggctatctgc ctcgggcatg aacaccaacg gcagatagag aaaagcccca
     1021 gttaacatta cgcgtcctgc aagacgctta acattaatct gaggctcaat ccatgctgaa
     1081 cacatgtagg ttagcctctt acgtgccgaa aggcaaggag aagcaggcta tgaagcagca
     1141 aaaggcgatg ttaatcgccc tgatcgtcat ctgtttaacc gtcatagtga cggcactggt
     1201 aacgaggaaa gacctctgcg aggtgcgaat ccgaaccggt cagacggagg tcgctgtctt
     1261 cgtagactac gaatctgaga agtaagagac caggcggggg agtaatctcc cgccacctct
     1321 gatgtgtcag gcatcctcaa cgcacccgcg ctttaccata ctgaaaatgc tgtttgaatg
     1381 ttcatctctg aaagaggact atgaatgaaa aaggtattga ttgcagcact tatttccggt
     1441 gtgtcttttg gcgcttttgc acagcagggt ggtttccagg ggccagaagc agagcgttca
     1501 acagtagcgc aggcaaaaga actgaaggat gatgcatggg ttatccttga agggagcatc
     1561 gttaaaaaag tgggtgatga acgttatgag tttcgtgaca atagcgggac aattgtcacg
     1621 gatattgatg acagcgtatg ggccgggcag aatgtttctc cgaaagacaa agtaagaatt
     1681 gagggtgaaa ttgataaaga cctgagcagt gttgaagttg atgtaaaggc actgaaatta
     1741 ttaaagtaac cgcccctgct tgttaagccc gtcttactga cgggttttct gtttgtacat
     1801 tccggcgtat tgccttacaa ttcgcgcagt cagcctgaac aactgacacc tgctgtcacc
     1861 ggagaatccg atgacacaac acataaaatc ccacaattct gaagccgacc cggaaattaa
     1921 gcaggggagg cgttttcgtg cgcctcagta tggctggttt cactatctgt tctgtacgat
     1981 cgatgaggca gatatgcttc aagaggcgta tctgcgtcgc ggtgtccgtg tggagcggag
     2041 tctgaacgct gatcgtctga cctggaccgt ttctgtatat cttcctgttc gtgcacatct
     2101 gccacggaca catgcctgct accgtcagcg cgtctggagg taatgtgcgg gtattacttc
     2161 gacctgttct ggttccggaa ctcgggctgg tggtccttaa gcccggtcgt gaatcattgc
     2221 cagtttttca tcgcggcagg gtgctggtgg agccggaacc gaaaaacatg cgggcgctgc
     2281 catctggagc ggttcctgct gttcgccagc cgctggcgga agataaatca ctgctgccat
     2341 ttttcagcga tgagcgggtg attcgtgcag ctggcggcgc tggtgcactg tctgactggt
     2401 tattacgtca cgtgaaatcc tgccagtggc cacacggcga ttatcatcac agcgaaaccg
     2461 ttattcacag ttacggtgct ggcgcaatgg tgttgtgctg gcactgcgac aaccagctgc
     2521 gcgaccagac ctccgaatca cttgagcaac ttactcaaca aaatctgaca gcctggatga
     2581 ttgacgtcat acgccatgta atgaatggca cgcaggagcg
//
LOCUS       DAZKHF020000392         2618 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2201S31241
            SAMN43267800-rid25106413.denovo.394, whole genome shotgun sequence.
ACCESSION   DAZKHF020000392 DAZKHF020000000
VERSION     DAZKHF020000392.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267800
            Sequence Read Archive: SRR30307263
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2618)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2618)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 2618)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350581.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350581.2
            Genome Coverage       :: 151x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:21:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,652
            CDSs (total)                      :: 5,557
            Genes (coding)                    :: 5,320
            CDSs (with protein)               :: 5,320
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 237
            CDSs (without protein)            :: 237
            Pseudo Genes (ambiguous residues) :: 0 of 237
            Pseudo Genes (frameshifted)       :: 102 of 237
            Pseudo Genes (incomplete)         :: 130 of 237
            Pseudo Genes (internal stop)      :: 52 of 237
            Pseudo Genes (multiple problems)  :: 41 of 237
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2618
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267800-rid25106413.denovo.394"
                     /serotype="O 26:H11"
                     /isolate="2201S31241"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-01"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            complement(<1..165)
                     /locus_tag="ACHOSC_RS23675"
                     /old_locus_tag="pgaptmp_004735"
     CDS             complement(<1..165)
                     /locus_tag="ACHOSC_RS23675"
                     /old_locus_tag="pgaptmp_004735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel protein MscS"
                     /protein_id="HGU4550512.1"
                     /translation="MIAELFTNNALNLVIIFGSCAALILMSFWFRRGNRKRKGFLFHA
                     VQFLIYTIIIS"
     gene            409..666
                     /gene="ynaJ"
                     /locus_tag="ACHOSC_RS23680"
                     /old_locus_tag="pgaptmp_004736"
     CDS             409..666
                     /gene="ynaJ"
                     /locus_tag="ACHOSC_RS23680"
                     /old_locus_tag="pgaptmp_004736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2534 family protein"
                     /protein_id="HGU4550513.1"
                     /translation="MIMAKLKSAKGKKFLFGLLAVFIIAASVVTRATIGGVIEQYNIP
                     LSEWTTSMYVIQSSMIFVYSLVFTVLLAIPLGIYFLGGEEQ"
     gene            complement(716..1666)
                     /gene="uspE"
                     /locus_tag="ACHOSC_RS23685"
                     /old_locus_tag="pgaptmp_004737"
     CDS             complement(716..1666)
                     /gene="uspE"
                     /locus_tag="ACHOSC_RS23685"
                     /old_locus_tag="pgaptmp_004737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein UspE"
                     /protein_id="HGU4550514.1"
                     /translation="MAMYQNMLVVIDPNQDDQPALRRAVYLHQRIGGKIKAFLPIYDF
                     SYEMTTLLSPDERTAMRQGVISQRTAWIHEQAKYYLNAGVPIEIKVVWHNRPFEAIIQ
                     EVISGGHDLVLKMAHQHDRLEAVIFTPTDWHLLRKCPSPVWMVKDQPWPEGGKALVAV
                     NLASEEPYHNALNEKLVKETIELAEQVNHTEVHLVGAYPVTPINIAIELPEFDPSVYN
                     DAIRGQHLLAMKALRQKFGINENMTHVEKGLPEEVIPDLAEHLQAGIVVLGTVGRTGI
                     SAAFLGNTAEQVIDHLRCDLLVIKPDQYQTPVELDDEEDD"
     gene            complement(1818..2570)
                     /gene="fnr"
                     /locus_tag="ACHOSC_RS23690"
                     /old_locus_tag="pgaptmp_004738"
     CDS             complement(1818..2570)
                     /gene="fnr"
                     /locus_tag="ACHOSC_RS23690"
                     /old_locus_tag="pgaptmp_004738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007849744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate/nitrate reduction transcriptional
                     regulator Fnr"
                     /protein_id="HGU4550515.1"
                     /translation="MIPEKRIIRRIQSGGCAIHCQDCSISQLCIPFTLNEHELDQLDN
                     IIERKKPIQKGQTLFKAGDELKSLYAIRSGTIKSYTITEQGDEQITGFHLAGDLVGFD
                     AIGSGHHPSFAQALETSMVCEIPFETLDDLSGKMPNLRQQMMRLMSGEIKGDQDMILL
                     LSKKNAEERLAAFIYNLSRRFAQRGFSPREFRLTMTRGDIGNYLGLTVETISRLLGRF
                     QKSGMLAVKGKYITIENNDALAQLAGHTRNVA"
BASE COUNT          669 a          612 c          624 g          713 t
ORIGIN      
        1 actgataatt atggtgtaga ttaaaaattg caccgcatgg aataaaaaac cttttctttt
       61 acgatttccg cggcgaaacc aaaagctcat cagaatcaat gctgcgcagc taccgaaaat
      121 aatgaccaga ttaagcgcat tatttgtaaa cagttcagcg atcattgttt tatcaggctc
      181 ctccagataa ttgtcgtcat gccggaaacc cctggcgggg ctattttacc gcgacaattc
      241 attcagatca tcaatagtca gggaaggaag tagcaacatt agctaactaa tcaatgcgtt
      301 gattgtaaat ccagctaaga ggtgaggttt tcagagcaga caacggtgaa atgtcatggt
      361 attgttacgt ttaggtaaca agaaatttgt ctgcacaagg attacatcat gattatggcg
      421 aaactgaagt cagcgaaagg gaagaaattt ctctttggtt tgttggcggt tttcattatt
      481 gcggcgtcgg ttgtgactcg cgcgactatc ggcggcgtta tagaacagta caatattccg
      541 ctgtctgagt ggacgacatc aatgtatgtg attcagtcat cgatgatttt tgtttatagc
      601 ctggtcttta ctgtgttgct ggcaatcccg ttgggaattt atttccttgg cggcgaagag
      661 cagtaagtaa aaaataggcc cgataactcg ggccttgtca gttattgaag agtcgttaat
      721 cgtcttcttc gtcatccagt tcaacgggtg tctgatactg gtcaggttta atgaccaaca
      781 gatcgcagcg aagatgatca atcacctgtt ccgccgtgtt gccgaggaat gctgctgaaa
      841 taccggtgcg tcctaccgtg cccagaacca caatccccgc ctgtaagtgc tccgccaaat
      901 caggaatcac ctcttctggc agaccttttt ctacgtgcgt catgttttca ttaatgccga
      961 atttctgccg cagagctttc attgccagca aatgttgccc acgaatggca tcgttataaa
     1021 cgctcggatc aaattccggc agttcaatcg cgatattaat tggcgttacc ggataagcgc
     1081 caaccagatg aacttcggta tggttgactt gttctgccag ttcgatcgtc tctttgacca
     1141 gtttttcatt gagcgcatta tgatacggct cttcactggc gagattcacc gccaccagcg
     1201 ccttgcctcc ttccggccac ggctggtctt tcaccatcca caccgggctt gggcatttgc
     1261 gtaacagatg ccagtccgtt ggcgtaaaaa tcaccgcttc cagacggtca tgttggtgcg
     1321 ccatttttag caccaaatcg tgcccgccgc tgatcacttc ctgaatgatg gcttcgaagg
     1381 gacggttatg ccagaccact ttaatttcaa tgggaacgcc agcattgaga taatattttg
     1441 cctgctcgtg gatccaggct gtacgctggc tgatgacgcc ctgacgcata gcggtacgtt
     1501 cgtccgggga gagcagggtg gtcatttcgt atgagaagtc atagatcggc aaaaaggctt
     1561 taattttgcc accaatccgt tgatgtaaat aaacagctcg ccgcaatgct ggttggtcgt
     1621 cctggttagg atcgataaca acgagcatgt tctgatacat agccatacag ggtctcctta
     1681 caacaactgt caacgcagtt tgtaattaaa agattaaccc atatctggtg aatgaaacag
     1741 tgatgaacct tctgccagat caataaatca gaaaaattta atgatatgac agaaggatag
     1801 tgagttatgc ggaaaaatca ggcaacgtta cgcgtatgac cagcaagctg ggccagcgca
     1861 tcgttatttt cgatggtgat gtatttacct ttgactgcca gcataccgct tttctggaag
     1921 cgacccagca gacggctgat ggtttctacc gtcaggccca gatagttacc gatatcgcca
     1981 cgagtcatcg tcaggcggaa ttcacgaggg gagaagccgc gttgggcaaa acgacgggac
     2041 aggttgtaga tgaatgcagc cagacgttcc tcggcatttt tcttcgacaa cagcaagatc
     2101 atgtcctgat cgcctttgat ttcaccgctc atcagacgca tcatctgctg acgcagattc
     2161 ggcattttac cggacaaatc gtccagcgtt tcgaacggga tttcacatac catcgaggtt
     2221 tccagcgcct gcgcgaagct cgggtgatgg ccgctgccga tggcgtcaaa tcccaccagg
     2281 tcgccagcta aatggaaacc agtgatttgc tcgtcgcctt gctcagtgat ggtataactt
     2341 ttaatcgtac cggagcggat ggcataaagc gatttaagtt catcaccagc cttaaacagc
     2401 gtctggcctt tctgaatagg cttcttccgc tcaatgatat tatcaagctg atcaagctca
     2461 tgttcgttga gtgtgaacgg gatgcaaagc tggctgatgc tgcaatcctg gcaatggata
     2521 gcacaaccgc cagactgaat gcgccgtata attcgctttt ccgggatcat aggtctgctc
     2581 aagccgtaat tgatatttgt caattttaac atcttttt
//
LOCUS       DAZKHF020000393         2600 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2201S31241
            SAMN43267800-rid25106413.denovo.395, whole genome shotgun sequence.
ACCESSION   DAZKHF020000393 DAZKHF020000000
VERSION     DAZKHF020000393.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267800
            Sequence Read Archive: SRR30307263
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2600)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2600)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 2600)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350581.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350581.2
            Genome Coverage       :: 151x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:21:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,652
            CDSs (total)                      :: 5,557
            Genes (coding)                    :: 5,320
            CDSs (with protein)               :: 5,320
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 237
            CDSs (without protein)            :: 237
            Pseudo Genes (ambiguous residues) :: 0 of 237
            Pseudo Genes (frameshifted)       :: 102 of 237
            Pseudo Genes (incomplete)         :: 130 of 237
            Pseudo Genes (internal stop)      :: 52 of 237
            Pseudo Genes (multiple problems)  :: 41 of 237
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2600
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267800-rid25106413.denovo.395"
                     /serotype="O 26:H11"
                     /isolate="2201S31241"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-01"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            <1..337
                     /locus_tag="ACHOSC_RS23695"
                     /old_locus_tag="pgaptmp_004739"
     CDS             <1..337
                     /locus_tag="ACHOSC_RS23695"
                     /old_locus_tag="pgaptmp_004739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF1376 domain-containing protein"
                     /protein_id="HGU4550516.1"
                     /translation="NPPRGNRGVKKFDPLDIALPNWISVSLWREWVEFRQALRKPIRT
                     EQGANGAIRELEKFRQQGFSPEQVIRHSIANEYQGLFAPKGVRPETLLRQVNTVSLPD
                     SAIPPGFRG"
     gene            344..1090
                     /locus_tag="ACHOSC_RS23700"
                     /old_locus_tag="pgaptmp_004740"
     CDS             344..1090
                     /locus_tag="ACHOSC_RS23700"
                     /old_locus_tag="pgaptmp_004740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HGU4550517.1"
                     /translation="MKNIATGDVLERIRRLAPSHVTAPFKTVAEWREWQLSEGQKRCE
                     EINRQNRQLRVEKILNRSGIQPLHRKCSFSNYQVQNEAQRYALSQAKSIADELMTGCT
                     NFAFSGKPGTGKNHLAAAIGNRLLKDGQTVIVVTVADVMSALHASYDDGQSGEKFLRE
                     LCEVDLLVLDEIGIQRETKNEQVVLHQIVDRRTASMRSVGMLTNLNYEAMKTLLGERI
                     MDRMTMNGGRWVNFNWESWRPNVVQPGIEK"
     gene            1113..1874
                     /locus_tag="ACHOSC_RS23705"
                     /old_locus_tag="pgaptmp_004741"
     CDS             1113..1874
                     /locus_tag="ACHOSC_RS23705"
                     /old_locus_tag="pgaptmp_004741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309973.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1627 domain-containing protein"
                     /protein_id="HGU4550518.1"
                     /translation="METVFDALKAMGKATSIELAARLDISREEVLNELWELKKAGFVD
                     KSAYTWRVADNNVQQEQPMQAELPEETTTATVVKISECDLTATIEQRGPQTADELATL
                     FGTTSRKVASTLAMAISKGRLIRVNQNGKFRYCMPGDNLPAEPKAASVAETDGKAFPQ
                     PAGVALPVQEAATQEDIKTETVADIVQSLPSFTETQADDLVLPSLHMANRELRRAKNH
                     VQKWERVCAALRELNKHRDIVRQIVDSSSRIVSEK"
     gene            1890..2312
                     /locus_tag="ACHOSC_RS23710"
                     /old_locus_tag="pgaptmp_004742"
     CDS             1890..2312
                     /locus_tag="ACHOSC_RS23710"
                     /old_locus_tag="pgaptmp_004742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016245379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF977 family protein"
                     /protein_id="HGU4550519.1"
                     /translation="MAKVFTQEEREKIKEQVVELVRQSGRETLRQLEAKTGATRYLMS
                     ILARELVASGDVYNSGYGLFPSEQARKDWKNARKKLSRAKVKKTVVVDPDLIWSLPDG
                     EIRRYDRRQNIICRESRRSEVMQRVLAFYRGNFQEVME"
     gene            2309..>2600
                     /locus_tag="ACHOSC_RS23715"
                     /old_locus_tag="pgaptmp_004743"
     CDS             2309..>2600
                     /locus_tag="ACHOSC_RS23715"
                     /old_locus_tag="pgaptmp_004743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001266128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4406 domain-containing protein"
                     /protein_id="HGU4550520.1"
                     /translation="MRVRVYIAGPMTGYENFNREAFHKVEEELKREGHTVLNPAVLPD
                     GLTQPHYMDICMAMIRCVDAIYMLNGWQRSAGAKAELALAEKLGHAVIYQEVA"
BASE COUNT          698 a          570 c          775 g          557 t
ORIGIN      
        1 aaatcccccc cgggggaatc gaggtgtcaa aaagtttgac cctctggata ttgctttgcc
       61 gaactggatt tctgtctcgc tttggcgtga gtgggttgaa tttcgccagg cattgcgaaa
      121 accgattcga acggagcagg gcgctaacgg ggcgatacgg gagctggaaa aattccgcca
      181 gcagggtttt tcacctgagc aggtgattcg acacagcatc gccaatgaat accagggctt
      241 gttcgcgccg aaaggtgttc gacctgagac gttactccga caggttaaca ccgtctcgtt
      301 accggatagt gcgatcccgc caggcttcag ggggtaactg accatgaaaa atattgcgac
      361 aggcgacgtt cttgaacgta tccgcagact ggccccgtca catgtaaccg cgccattcaa
      421 gacggtagcg gagtggcgcg agtggcaact ttccgaaggc cagaaacgtt gtgaggagat
      481 caaccgtcag aatcgtcagt tgcgggtgga aaaaattctg aatcgctctg gcatccagcc
      541 attgcaccgc aaatgctcgt tttcgaatta ccaggtgcag aacgaagcgc agcgatacgc
      601 gttgagtcag gcgaaatcca tcgctgatga actgatgacc gggtgtacaa attttgcgtt
      661 cagcggaaaa cctggtaccg ggaagaacca cttagcggca gctatcggga atcgcctgct
      721 gaaagacggt cagacagtga ttgtggttac cgtggctgat gttatgagtg ccctgcacgc
      781 cagctatgac gatgggcagt caggcgaaaa atttttgcgg gaactatgcg aagtggatct
      841 gctggttctt gatgaaattg gcattcagcg cgagacgaaa aacgagcagg tggtgctgca
      901 ccagattgtt gatcgccgga cagcgtcgat gcgcagcgtg gggatgctga caaacctgaa
      961 ctatgaggcc atgaaaacat tgctcggcga gcggattatg gatcgcatga ccatgaacgg
     1021 cgggcgatgg gtgaatttta actgggagag ctggcgtccg aatgtcgtcc agccaggaat
     1081 tgagaagtaa tttttaccgg gaggaaattt ttatggagac tgtttttgac gcactgaaag
     1141 caatgggaaa agccacatcc atagaacttg ctgcgcgact tgatatcagt cgtgaagaag
     1201 tgctgaacga actatgggaa ctgaaaaaag ctggtttcgt tgataaaagc gcgtacacct
     1261 ggcgtgtggc tgataacaac gttcagcagg aacagccaat gcaggcagaa ctgccggaag
     1321 aaaccaccac ggcaactgta gtgaaaatct cagagtgcga tttaaccgcg acgattgaac
     1381 aacgcggacc acaaacggcg gatgaactgg ctacgctgtt cggtacaaca tcccgcaaag
     1441 ttgcttcaac gctggcaatg gcaatcagca aaggacgcct gattcgcgta aaccagaacg
     1501 gtaaatttcg ttactgcatg ccgggcgata atttaccagc agagccgaaa gctgcatcgg
     1561 tagcggaaac tgatggtaaa gcctttcctc agccagccgg tgttgcgtta ccagtacagg
     1621 aagcggcaac acaggaagat attaaaacag aaactgtggc ggacattgtg cagtcgctgc
     1681 catcgtttac tgaaacgcaa gcggatgacc tagttttacc atcgctgcat atggcaaacc
     1741 gcgaactgcg tcgggcgaaa aatcatgtcc agaagtggga gcgagtctgc gccgcgctgc
     1801 gggagctgaa caagcaccgg gatattgtcc gacagattgt cgattcctcc agtcgtattg
     1861 tgtcggaaaa gtgattgccg gaggcgctta tggcaaaagt ttttacacag gaagagcgtg
     1921 aaaaaattaa ggagcaggtt gttgagttgg tacgccagag tgggcgcgag acgttaaggc
     1981 aactggaggc caagacaggt gcgacaagat atctgatgag cattcttgcc agagagttgg
     2041 ttgccagtgg cgatgtatac aactctggct atgggttatt cccgtctgaa caggctcgta
     2101 aggattggaa aaacgcccgc aaaaaactat ccagggcaaa ggtgaaaaaa acggttgtgg
     2161 ttgatcctga ccttatctgg tcattaccag acggagaaat acgccgctat gacaggcgtc
     2221 agaacataat ttgtcgtgag agcaggagga gcgaagttat gcagcgtgtg ctggcgttct
     2281 atcggggaaa ctttcaggag gtgatggagt gagggtgaga gtttatattg ccggtccaat
     2341 gacgggatat gaaaatttca accgtgaggc atttcacaag gtggaagagg aactgaaacg
     2401 ggaagggcat accgtcttaa acccggcagt acttccggac gggctgacac agccgcacta
     2461 catggatatt tgcatggcaa tgattcgttg tgtggatgcg atttacatgc tgaatggctg
     2521 gcagcggtca gcgggcgcta aggcagagct ggcactggcg gagaaactgg ggcatgcggt
     2581 gatttatcag gaggtggctc
//
LOCUS       DAZKHF020000394         2597 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2201S31241
            SAMN43267800-rid25106413.denovo.396, whole genome shotgun sequence.
ACCESSION   DAZKHF020000394 DAZKHF020000000
VERSION     DAZKHF020000394.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267800
            Sequence Read Archive: SRR30307263
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2597)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2597)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 2597)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350581.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350581.2
            Genome Coverage       :: 151x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:21:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,652
            CDSs (total)                      :: 5,557
            Genes (coding)                    :: 5,320
            CDSs (with protein)               :: 5,320
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 237
            CDSs (without protein)            :: 237
            Pseudo Genes (ambiguous residues) :: 0 of 237
            Pseudo Genes (frameshifted)       :: 102 of 237
            Pseudo Genes (incomplete)         :: 130 of 237
            Pseudo Genes (internal stop)      :: 52 of 237
            Pseudo Genes (multiple problems)  :: 41 of 237
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2597
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267800-rid25106413.denovo.396"
                     /serotype="O 26:H11"
                     /isolate="2201S31241"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-01"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            47..1477
                     /locus_tag="ACHOSC_RS23720"
                     /old_locus_tag="pgaptmp_004744"
     CDS             47..1477
                     /locus_tag="ACHOSC_RS23720"
                     /old_locus_tag="pgaptmp_004744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdgA family protein"
                     /protein_id="HGU4550521.1"
                     /translation="MIRKSATGVIVALAVIWGGGTWYTGTQIQPGIEKFIKDFNDAKK
                     KGEHAYDMTLSYKNFDKGFFNSRFQMQMTFDNGAPDLNIKPGQKVVFDVDVEHGPLPI
                     TMLMHGNVIPALAAAKVNLVNNELTQPLFIAAKNKSPVEATLRFAFGGSFSTTLDVAP
                     AEYGKFSFGEGQFTFNGDGSSLSNLDIEGKVEDIVLQLSPMNKVTAKSFTIDSLARLE
                     EKKFPVGESESKFNQINIINHGEDVAQIDAFVAKTMLDRVKDKDYINVNLTYELDKLT
                     KGNQQLGSGEWSLIAESIDPSAVRQFIIQYNIAMQKQLAAHPELANDEVALQEVNAAL
                     FKEYLPLLQKSEPTIKQPVKWKNALGELNANLDISIADPAKSSSSTNKDIKSLNFDVK
                     LPLNVVTETAKQLNLSEGMDAEKAQKRADKQISGMMTLGQMFQLITIDNNTASLQLRY
                     TPGKVVFNGQEMSEEEFMSRAGRFVH"
     gene            complement(1518..2450)
                     /locus_tag="ACHOSC_RS23725"
                     /old_locus_tag="pgaptmp_004745"
                     /pseudo
     CDS             complement(1518..2450)
                     /locus_tag="ACHOSC_RS23725"
                     /old_locus_tag="pgaptmp_004745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312812.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase"
BASE COUNT          812 a          533 c          568 g          684 t
ORIGIN      
        1 ataagtggat attcaaagtt ttgctgtttt atcagggaat atttatatga tacgtaagtc
       61 agctacaggt gttattgttg cgttagccgt aatctggggt ggtggcacat ggtacacagg
      121 tacgcaaatt cagcctggta tcgaaaagtt tattaaagat tttaacgatg cgaaaaagaa
      181 aggtgaacat gcctacgata tgacgttaag ttataaaaac tttgacaaag gcttttttaa
      241 ttcccgtttt caaatgcaaa tgacattcga taacggtgca cccgatctca atatcaagcc
      301 aggccagaaa gttgtatttg atgtggatgt tgagcacggt ccgttgccca tcacaatgtt
      361 aatgcatggt aatgtcatcc cagcactggc agcggcaaaa gtgaacttag tgaataatga
      421 actgacacaa ccgctattta tcgccgcgaa aaataaatcg cccgtggaag cgacattgcg
      481 attcgcgttt ggtggctcat tctctacgac attagatgtt gcccctgcag agtatggaaa
      541 gttttctttt ggtgagggcc agtttacttt taatggtgat ggtagttcat tgtctaacct
      601 ggatattgaa ggcaaagtcg aagatattgt tctgcaatta tcaccaatga acaaagtaac
      661 ggcaaaaagt tttaccattg attctctggc gcgattagaa gaaaagaaat ttccggttgg
      721 tgaaagcgag tcgaaattta atcagattaa cattatcaat cacggggaag acgttgccca
      781 aatcgatgct ttcgttgcaa aaaccatgct ggatcgcgtt aaagacaaag attatatcaa
      841 tgtcaatctg acctacgaac ttgataagtt aacaaaaggg aatcagcaac tcggtagtgg
      901 tgagtggtca ttgattgctg aatctattga tccctcagcg gtgcgccaat ttatcatcca
      961 gtataacatt gcaatgcaga agcagcttgc tgcacaccct gagttagcaa acgatgaagt
     1021 tgctctgcaa gaagtgaatg ctgcattgtt caaagagtat ttaccgttat tacaaaaaag
     1081 tgagccgacc attaaacaac cggtaaaatg gaagaacgca ctcggcgaac taaatgccaa
     1141 tctggatatc agtattgccg acccagccaa atcttcatca tccacaaaca aagatattaa
     1201 atcgctcaat tttgatgtga agttaccgct taatgtcgtc acagaaaccg caaaacagct
     1261 taatttatct gaaggaatgg atgcggaaaa agcgcaaaag cgggctgata aacaaatcag
     1321 cgggatgatg accttaggtc agatgtttca gttaatcacg attgacaaca ataccgcctc
     1381 gctgcaactg cgttatacac cgggtaaagt tgtttttaac ggacaggaga tgagcgaaga
     1441 agaatttatg tctcgtgccg gacgttttgt tcattaaaat actcactcgc ctctgctcat
     1501 tcagaggcga gcaaaattta cttgtcctgc cgctgtgatg acatcaacga cgtcagaagt
     1561 tggtcttttt cctgccacag tgagttcagc cattgctgaa aatggcgctt aaagcttttg
     1621 tcattgatgt agtcgccatg taactcatcg gcaataggct gtaaatccac atgaacgaca
     1681 attcgcgtca gtttaccgct taacatatcg aagaacggct gacggttatt gtccggataa
     1741 cacagcgtaa cattaagcag tttatcgaat tgtttaccca gtacatttag cgccatcgca
     1801 atgcctgccg cctttggtgg caacaagttt tgaaaagtgg agtgggtttg ctgatgtttt
     1861 tcttgcgtga aacgggagcc ttcaacgaaa ttgacaatgg tggtgggatg caggcgaaac
     1921 ttttcacaag aacgccgagt ggtttcaaca tctttaccac gtcgctccgg atgacgtaat
     1981 aaataagcgc gggaataacg cttcataaac ggcatatcca gcgcccagca cgccaggcca
     2041 agaaatggca cctaggccag ctgttgcttg aggaaatatt tattcatcgg aatgtgctta
     2101 cgaaacagca cgcacagtac gacaatatct gcccaactac ggtggttaca aataagcaga
     2161 taccagttct tcttacttaa cccttccagc ccgtgaactt cccattgcag gtgtgggttc
     2221 agatgcagta ataccgccag accttcacac cagcaataca tcataaaatc acaaaaacgc
     2281 gaaacctttc gccaaatgac tggcacaggt agcaatagtt ttacaatccc ggcaatgatg
     2341 atcgggacgg aacaaaaaat agtgaccaaa atggtcaata cgatactcag caaaagggtt
     2401 atcgcagcga gtattctcgt cataatgaat ttattcaaaa ggttagccat agacagtgcg
     2461 ctaaagaagc gcaaggcgca gattttagca gaaaacgacc caaataacgg atgatcctta
     2521 aggagaaaaa taattcatat ctatccacat tagaaaaaat cccattatct caattattag
     2581 ggatggcttt attttta
//
LOCUS       DAZKHF020000395         2588 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2201S31241
            SAMN43267800-rid25106413.denovo.397, whole genome shotgun sequence.
ACCESSION   DAZKHF020000395 DAZKHF020000000
VERSION     DAZKHF020000395.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267800
            Sequence Read Archive: SRR30307263
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2588)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2588)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 2588)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350581.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350581.2
            Genome Coverage       :: 151x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:21:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,652
            CDSs (total)                      :: 5,557
            Genes (coding)                    :: 5,320
            CDSs (with protein)               :: 5,320
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 237
            CDSs (without protein)            :: 237
            Pseudo Genes (ambiguous residues) :: 0 of 237
            Pseudo Genes (frameshifted)       :: 102 of 237
            Pseudo Genes (incomplete)         :: 130 of 237
            Pseudo Genes (internal stop)      :: 52 of 237
            Pseudo Genes (multiple problems)  :: 41 of 237
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2588
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267800-rid25106413.denovo.397"
                     /serotype="O 26:H11"
                     /isolate="2201S31241"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-01"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            complement(<1..>2588)
                     /gene="eae"
                     /locus_tag="ACHOSC_RS23730"
                     /old_locus_tag="pgaptmp_004746"
     CDS             complement(<1..>2588)
                     /gene="eae"
                     /locus_tag="ACHOSC_RS23730"
                     /old_locus_tag="pgaptmp_004746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000627895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="intimin type beta"
                     /protein_id="HGU4550522.1"
                     /translation="AQDRLFYTLKTGETVANISKSQGISLSVIWSLNKHLYSSESEMM
                     KAGPGQQIILPLKKLSVEYSALPVLGSAPVVAAGGVAGHTNKMTKMSPDATKSNTTDD
                     KALNYAAQQAASLGSQLQSRSLNGDYAKDTALGMASSQASSQLQAWLQHYGTAEVNLQ
                     SGNNFDGSSLDFLLPFYDSENMLAFGQVGARYIDSRFTANLGAGQRFFLPENMLGYNV
                     FIDQDFSGDNTRLGIGGEYWRDYFKSSVNGYFRMSGWHESYNKKDYDERPANGFDIRF
                     NGYLPSYPALGAKLMYEQYYGDNVALFNSDKLQSNPGAATVGVNYTPIPLVTMGIDYR
                     HGTGNENDLLYSMQFRYQFDKPWSQQIEPQYVNELRTLSGSRYDLVQRNNNIILEYKK
                     QDILSLNIPHDINGTEHSTQKIQLIVKSKYGLDRIVWDDSALRSQGGQIQHGGSQSAQ
                     DYQAILPAYVQGGSNIYKVTARAYDRNGNSSNNVQLTITVLPNGQVVDQVGVTDFTAD
                     KTSAKADGIEAITYTATVKKNGVAQANVPVTFSIVSGTATLGANSARTDGNGKATVTL
                     KSATPGQVVVSAKTAEMTSPLNASAVIFVDQTKASITEIKADKTTAKADGSDAITYTV
                     RVMKEGAPVVDQKVTFSKDFGTLNKTEATTDQNGYATVKLSSNTPGKAIVSAKVSGVG
                     TEVKATTVEFFAPLSIDGDKVTVIGTGITGALPKNWLQYGQVKLQATGGNGKYTWKSS
                     NTKIASVDNSGVITLNEKGSATITVVSGDNQSATYTINAPGSIVIAVDKNTRVTYFDA
                     ENKCKTNSANLAQSKELLANIYSTWGAANKYPYYSGSKSLTAWIKQSSSEQSSGVSST
                     YDLVTK"
BASE COUNT          700 a          628 c          514 g          746 t
ORIGIN      
        1 tcttcgtaac caaatcatat gtgcttgata cacctgatga ctgttcagaa gaggattgtt
       61 taatccaagc agtcaatgat ttagaaccag aatagtaagg atatttattt gcagcacccc
      121 atgttgaata gatattggcc aatagttctt ttgactgtgc taaatttgcg ctatttgtct
      181 tacatttgtt ttcggcatca aaatacgtaa ctcgagtatt tttatccaca gcaattacaa
      241 tactacccgg tgcattaatt gtgtatgtcg cactctgatt atcaccagat actacagtaa
      301 ttgtggcact ccctttttca tttaaggtta tcactcccga gttatcaaca gaagcaattt
      361 tagtattact ggatttccat gtgtattttc cattgccccc tgttgcctgt agcttaacct
      421 gaccatactg taaccagttc tttggcagag cccccgtgat accagtacca attacggtca
      481 ctttatcacc atcaatactc aacggggcaa aaaactcaac ggtagtagcc ttaacttctg
      541 tacctactcc actcactttt gcactaacaa tggccttgcc aggagtattg gatgataatt
      601 ttacagtagc ataaccattc tgatcggttg ttgcttcagt cttattcagg gtcccaaaat
      661 ccttagaaaa ggtcactttc tgatctacta cgggtgcccc ctccttcatc actctgacag
      721 tataggtaat cgcatcagaa ccatctgcct tcgctgttgt tttatcagcc ttaatctcag
      781 taatactggc cttggtttga tcaacaaata taaccgcgct ggcattaagt ggcgaagtca
      841 tctccgcggt tttagcagac acgacgacct gtcctggcgt agccgacttc agcgttacgg
      901 tcgccttacc gttaccatcc gttctggcac tatttgcccc aagagttgca gtcccggata
      961 caatactaaa tgttacaggg acattagcct gagctacacc attcttttta accgtcgcgg
     1021 tataggtaat agcttctatg ccatccgctt tagccgatgt tttatcagcc gtaaagtccg
     1081 ttaccccaac ctggtccaca acctgcccat tcggtaaaac ggtaatagtg agctgtacat
     1141 tattagaact attaccattt cggtcatagg cgcgagcggt cactttataa atattgctgc
     1201 cgccttgcac ataagcaggc aaaatagcct ggtagtcttg tgcgctttgg cttccgccat
     1261 gctgaatctg accgccctga ctgcgtaatg cgctatcatc ccagacgata cgatccagac
     1321 cgtatttgct cttaacgatc aattgaatct tctgcgtact gtgttcagta ccattaatat
     1381 catgcggaat attcagagaa agaatatcct gctttttgta ctccagaata atattgttat
     1441 tacgctgaac cagatcgtaa cggctgcccg ataatgttct taactcgtta acatactgtg
     1501 gctcgatttg ctgagaccac ggtttatcaa actgataacg gaactgcatt gagtaaagga
     1561 gatcattttc attacccgta ccatgacggt aatcgatccc catcgtcacc agaggaatcg
     1621 gagtgtagtt tacaccaacg gtcgccgcgc caggattcga ctgcaactta tcggaattaa
     1681 acaaagcaac attatcacca taatactgtt cgtacatcag tttggcgcct aatgccggat
     1741 atgatggtaa atagccatta aagcggatat caaaaccatt tgccgggcgc tcatcatagt
     1801 ctttcttatt gtatgactca tgccagccgc tcatgcggaa atagccgtta acgctacttt
     1861 tgaaatagtc tcgccagtat tcgccaccaa tacctaaacg ggtattatca ccagaaaaat
     1921 cctgatcaat gaagacgtta tagcccaaca tattttcagg aaggaaaaaa cgctggccag
     1981 cacctaaatt tgccgtaaag cgggagtcaa tgtaacgcgc accgacctga ccaaatgcca
     2041 gcatgttttc ggaatcatag aacggtaata agaagtccag tgaactaccg tcaaagttat
     2101 taccactctg cagattaacc tctgccgttc cataatgttg taaccaggcc tgcaactgtg
     2161 acgaagcctg gctgctggcc ataccaagag cggtatcttt cgcgtaatcg ccgttcagtg
     2221 agcgcgactg gagctggcta ccaaggctcg cggcctgttg tgccgcataa tttagagcct
     2281 tgtcatcggt cgtgttgctt ttagtcgcgt ccggggacat tttagtcatt ttattcgtat
     2341 gaccagcgac accacctgca gcaacaacag gtgccgaacc taagacaggt aaggcactat
     2401 attcaacaga cagttttttg agtggcaaaa tgatctgctg accaggtcca gccttcatca
     2461 tttcgctttc ggaactgtat aaatgtttat tcagtgacca aattaccgat aaactgatac
     2521 cctgtgattt agaaatattg gcaacagttt cacctgtttt taacgtataa aaaaggcgat
     2581 cctgagcg
//
LOCUS       DAZKHF020000396         2538 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2201S31241
            SAMN43267800-rid25106413.denovo.398, whole genome shotgun sequence.
ACCESSION   DAZKHF020000396 DAZKHF020000000
VERSION     DAZKHF020000396.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267800
            Sequence Read Archive: SRR30307263
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2538)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2538)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 2538)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350581.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350581.2
            Genome Coverage       :: 151x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:21:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,652
            CDSs (total)                      :: 5,557
            Genes (coding)                    :: 5,320
            CDSs (with protein)               :: 5,320
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 237
            CDSs (without protein)            :: 237
            Pseudo Genes (ambiguous residues) :: 0 of 237
            Pseudo Genes (frameshifted)       :: 102 of 237
            Pseudo Genes (incomplete)         :: 130 of 237
            Pseudo Genes (internal stop)      :: 52 of 237
            Pseudo Genes (multiple problems)  :: 41 of 237
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2538
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267800-rid25106413.denovo.398"
                     /serotype="O 26:H11"
                     /isolate="2201S31241"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-01"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            79..300
                     /locus_tag="ACHOSC_RS23735"
                     /old_locus_tag="pgaptmp_004747"
     CDS             79..300
                     /locus_tag="ACHOSC_RS23735"
                     /old_locus_tag="pgaptmp_004747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4550523.1"
                     /translation="MKRLIPVALLTALLAGCAHDSPCVPVYDDQGRLVHTNTCMKGTT
                     QDNWETAGAIAGGAAAVAGLTMGIIALSK"
     gene            731..1756
                     /locus_tag="ACHOSC_RS23740"
                     /old_locus_tag="pgaptmp_004748"
     CDS             731..1756
                     /locus_tag="ACHOSC_RS23740"
                     /old_locus_tag="pgaptmp_004748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter substrate-binding
                     protein"
                     /protein_id="HGU4550524.1"
                     /translation="MKKMMIATLAAASVLLAVANQAHAGATLDAVQKKGFVQCGISDG
                     LPGFSYADADGKFSGIDVDVCRGVAAAVFGDDTKVKYTPLTAKERFTALQSGEVDLLS
                     RNTTWTSSRDAGMGMAFTGVTYYDGIGFLTHDKAGLKSAKELDGATVCIQAGTDTELN
                     VADYFKANNMKYTPVTFDRSDESAKALESGRCDTLASDQSQLYALRIKLSNPAEWIVL
                     PEVISKEPLGPVVRRGDDEWFSIVRWTLFAMLNAEEMGINSQNVDEKAANPATPDMAH
                     LLGKEGDYGKDLKLDNKWAYNIIKQVGNYSEIFERNVGSESPLKIKRGQNNLWNNGGI
                     QYAPPVR"
     gene            1824..2213
                     /locus_tag="ACHOSC_RS23745"
                     /old_locus_tag="pgaptmp_004749"
                     /pseudo
     CDS             1824..2213
                     /locus_tag="ACHOSC_RS23745"
                     /old_locus_tag="pgaptmp_004749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312169.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
     gene            2268..>2538
                     /locus_tag="ACHOSC_RS23750"
                     /old_locus_tag="pgaptmp_004750"
     CDS             2268..>2538
                     /locus_tag="ACHOSC_RS23750"
                     /old_locus_tag="pgaptmp_004750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HGU4550525.1"
                     /translation="MTKNTRFSPEVRQRAIRMVLESQDEYDSQWAAICSIAPKIGCTP
                     ETLRVWVRQHERDTGGGDGGLTSAERQRLKELERENRELRRSNDIL"
BASE COUNT          615 a          586 c          652 g          685 t
ORIGIN      
        1 cggtgataaa atggcattca gctcgttaat aagagagtta acttattaag cgttagcgtt
       61 tattactgag gtaacaccat gaaaagatta attcctgtgg cattgctcac cgcattgctg
      121 gcgggctgtg ctcacgattc gccctgcgtt ccggtttatg acgatcaggg tcgtctggtt
      181 cacaccaata cctgtatgaa aggcacgact caggataact gggaaacggc tggggctatt
      241 gccggcgggg cagcggctgt tgctggcctg acgatgggga tcatcgctct gtcgaaataa
      301 ctgaacaggg cgcggagttt cgcgctctgt ttaacgatac agtatcttta cataagataa
      361 aatattgcta tattctgctg gctaattctc tgctgattta gcatgttata tcgcgttatt
      421 tcgctgcttt cgaaataacg catcaattta actttcacac ttcccaccag aaatatactc
      481 acgctaacaa tcattattct gcttagtaaa acacgcgtaa atattttgcg ccaaaatgtg
      541 gcgcatgttt cattttcgca ccattgcggg gcgctgtttt tattatttcc tgtctacact
      601 cagcttattg cgacgcgaaa tcgggcgcaa aagctggcac tacttttgct tataagagga
      661 tggccacaga caggtaaaag acgtttccca aacgtcctat aacgataatt ttcgcctcac
      721 aggaagcatt atgaaaaaga tgatgatagc cacactggct gccgccagcg tgctgcttgc
      781 cgttgcaaat caggcgcatg ctggagcgac gcttgatgcc gttcagaaaa aaggttttgt
      841 gcaatgcggg atcagtgatg gattacctgg gttctcttat gccgatgcag acggtaagtt
      901 ttcaggtatt gatgttgatg tttgtcgtgg tgttgccgct gctgtatttg gtgacgacac
      961 gaaagtgaaa tataccccac tcacagcaaa agaacgcttc acggctttac agtcagggga
     1021 agtggatttg ctctcccgta atacgacctg gacttcatct cgcgacgccg ggatgggaat
     1081 ggcatttact ggcgtcactt attacgacgg cattggcttc ctgacgcacg ataaagcggg
     1141 gctaaaaagc gcgaaagaac tggatggcgc taccgtctgt attcaggcgg gtactgatac
     1201 cgaactcaac gttgccgact atttcaaggc aaacaatatg aagtacacac cggtgacttt
     1261 cgatcgctct gacgaatcag cgaaggcact ggaatctggt cgctgcgata cgctggcctc
     1321 ggatcagtcg caactgtatg ccctgcgcat caaattaagc aacccagctg aatggattgt
     1381 cttaccggag gttatctcta aagaaccgct tgggccggta gttcgtcgtg gcgatgatga
     1441 atggttctcg attgtacgct ggacgctttt cgccatgctg aatgctgaag agatgggcat
     1501 caattcccag aacgtcgatg aaaaagcggc taatccagca acgcctgata tggcacatct
     1561 gctgggtaaa gaaggcgatt acggcaagga tctgaagttg gataataaat gggcctataa
     1621 catcatcaaa caggtgggta actactcgga aattttcgag cgtaatgtag gttcagaaag
     1681 cccgctgaaa attaaacgtg ggcaaaataa tctctggaat aacggcggta ttcagtacgc
     1741 gccgcccgtg cgttaagtcg gtgtgaggta acgagcgtcg ctacggcggc gctctcttca
     1801 gcgttatgct ttccgaggtt tctatgtctc atcgccgctc aaccgttaaa ggctcactct
     1861 cctttgccaa ccctacggtt cgtgcctggt tattccagat ccttgccgtt gttgctgttg
     1921 tcggcattgt tggttggtta tttcacaaca ctgtaacgaa tctcagtaat cgtggcatta
     1981 cttcaggttt tgcctttctg gatcgcggcg ctggcttcgg tattgtccag catttgatcg
     2041 attaccagca gggcgacacc tacggacgcg tttttattgt cggcttactc aatacgctac
     2101 tggtttctgc attgtgtatt gtgttcgctt ctgttttggg cttctttatc ggtctggcga
     2161 gactttcgga taactggctg ctacgaaagc tttccacaat ttatattgag atctgaaccg
     2221 ccccggaaat cctggagact aaactccctg agaaagaggt aaacaggatg actaaaaata
     2281 ctcgtttttc ccccgaagtc cgtcagcggg cgattcgtat ggttctggaa agtcaggatg
     2341 aatatgactc acagtgggcg gcaatttgtt ccattgcccc aaagattggc tgtacgccgg
     2401 agaccctgcg tgtctgggtt cgccagcatg agcgggatac cgggggcggt gatggtgggc
     2461 tcaccagcgc tgaacgtcag cgtctgaaag agctggaacg tgaaaatcgt gaactgcgcc
     2521 gcagtaacga tatccttc
//
LOCUS       DAZKHF020000397         2532 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2201S31241
            SAMN43267800-rid25106413.denovo.399, whole genome shotgun sequence.
ACCESSION   DAZKHF020000397 DAZKHF020000000
VERSION     DAZKHF020000397.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267800
            Sequence Read Archive: SRR30307263
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2532)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2532)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 2532)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350581.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350581.2
            Genome Coverage       :: 151x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:21:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,652
            CDSs (total)                      :: 5,557
            Genes (coding)                    :: 5,320
            CDSs (with protein)               :: 5,320
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 237
            CDSs (without protein)            :: 237
            Pseudo Genes (ambiguous residues) :: 0 of 237
            Pseudo Genes (frameshifted)       :: 102 of 237
            Pseudo Genes (incomplete)         :: 130 of 237
            Pseudo Genes (internal stop)      :: 52 of 237
            Pseudo Genes (multiple problems)  :: 41 of 237
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2532
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267800-rid25106413.denovo.399"
                     /serotype="O 26:H11"
                     /isolate="2201S31241"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-01"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            15..665
                     /gene="tnpA"
                     /locus_tag="ACHOSC_RS23755"
                     /old_locus_tag="pgaptmp_004751"
     CDS             15..665
                     /gene="tnpA"
                     /locus_tag="ACHOSC_RS23755"
                     /old_locus_tag="pgaptmp_004751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS66-like element accessory protein TnpA"
                     /protein_id="HGU4550526.1"
                     /translation="MPCFRSYLPDALRLRFEDKLTIRAIAQRLGLSHSTIHTLFQRFI
                     ASGIAWPLPDSVSLAQLDAILYANRKKELTEPQISEGTWRKERRASYSREFKVRLAKQ
                     ALQPGAVVARIAREHGINDNLLFKWKSQYEDGLLSDDDIQECMPVPVALTDTPEPTRP
                     VTNPFWRNKPDECPESDPGNVPRCELHLKSGVVKLFDPLTPEMLRALIREMKGGTR"
     gene            662..1009
                     /gene="tnpB"
                     /locus_tag="ACHOSC_RS23760"
                     /old_locus_tag="pgaptmp_004752"
     CDS             662..1009
                     /gene="tnpB"
                     /locus_tag="ACHOSC_RS23760"
                     /old_locus_tag="pgaptmp_004752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858152.1"
                     /note="TnpB, as the term is used for proteins encoded by
                     IS66 family insertion elements, is considered an accessory
                     protein, since TnpC, encoded by a neighboring gene, is a
                     DDE family transposase; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family insertion sequence element accessory
                     protein TnpB"
                     /protein_id="HGU4550527.1"
                     /translation="MITLPTGTRIWIIAGITDMRCGFNGLASKVQNTLKDDPFSGHIF
                     VFRGRSGKMVKILWADRDGLCLFAKRLERGRFVWPVTREGKVHLTPAQLSMLLEGIAW
                     QHPKRTERPGIRI"
     gene            1029..2532
                     /locus_tag="ACHOSC_RS23765"
                     /old_locus_tag="pgaptmp_004753"
                     /pseudo
     CDS             1029..2532
                     /locus_tag="ACHOSC_RS23765"
                     /old_locus_tag="pgaptmp_004753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611684.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family transposase"
BASE COUNT          640 a          686 c          707 g          499 t
ORIGIN      
        1 agacaaaaaa agatattccc tgcttccgtt cttatttgcc tgatgctctg cgtttaagat
       61 ttgaagataa actgaccatc cgggccatcg ctcagcgtct aggtctcagt cattccacaa
      121 tacatacgct ttttcagcga tttattgcat ccggtatcgc atggccattg cccgattcag
      181 tttcattagc tcagcttgat gccatccttt atgccaacag aaagaaggaa ttaacagagc
      241 ctcaaatcag cgaaggcaca tggcgaaaag aacggcgagc cagctacagc cgtgaattta
      301 aggtccgtct ggctaagcag gcattacagc ccggtgctgt tgttgcccgg atcgccagag
      361 agcacggtat caatgataac ctgctgttta aatggaaaag ccagtacgag gacggcttac
      421 tgagcgatga tgacatacag gaatgcatgc ctgtcccggt ggctctgact gatacgccag
      481 agccgaccag accagttaca aatcccttct ggcgtaacaa gcctgatgag tgccctgaga
      541 gtgatcccgg aaacgtccca cggtgcgagc tgcatcttaa atcaggtgtg gtaaaactgt
      601 ttgaccctct cactccggaa atgttacggg cgctaatccg cgaaatgaaa ggaggtaccc
      661 gatgataacg ctgccaaccg gtaccagaat ctggatcatc gctggcatca cagatatgcg
      721 ttgtggcttc aacggcctgg cttcgaaggt gcagaacacg ctgaaagatg acccgttctc
      781 cgggcatatc ttcgtcttcc ggggccgcag tggcaaaatg gtgaaaatac tgtgggccga
      841 tcgtgacggg ttatgcctgt tcgccaaacg cctggaacgg ggccgcttcg tctggccggt
      901 aacccgggaa gggaaagtgc acctgacgcc agctcagtta tccatgctac tggaggggat
      961 cgcgtggcaa catcccaaac ggacagaacg gcctggcatc cggatataac ccgtgataaa
     1021 acaggggaat gaacaacaca ctccccgacg acatcgagca actgaaggcc ctgctgatcg
     1081 cacagcaggc tgttatcgtc tgtctggtga aataaccggc tatgcccgcg agatcagctc
     1141 actcagagcg ctggtcgcta aactgcagag aatgttgttc ggtcgcagca gcgagaaaag
     1201 ccgcgagaag atagaaaaga agatcgcacg ggcagaaacg cgtataaccg agctccagaa
     1261 caggcttggt gaggcgcagt tgcaactcac ctcaatggcc ggagagacag cgccgaaaac
     1321 atcagactct cccgtccgca aagcacttcc ggcaacactt ccccgtgaca ggcaggttat
     1381 ctccccggca gaaaccgaat gccccgtctg cagcggcaaa ctgaaaccgc tgggagaaag
     1441 catctctgaa caactggata tcatcaacac cgcgttcagg gtaatcgaaa cggttcgccc
     1501 aaaactggcc tgcagccggt gcgactgtat agttcaggct ccgcagccac caaaacccat
     1561 cgagcgcagt tacgccagtc cggctctgct ggcccgcata atcatggcta agttcgccga
     1621 gcatctgccg ctgtaccgtc agtcggaaat ctatgcccgc cagggcgtgg agctgcaccg
     1681 caatacgatg gggcgctggg ttgacatcat gggagagcag cttcgcccgc tgtatgatga
     1741 actgaagcac tatgtgctga tggcgggtaa agtgcatgcc gatgacacgc cggtaaatgt
     1801 actggagccg ggtcagggta aaacccgtac cggacggctg tgggtctatg ttcgtgacga
     1861 tcgcaacgcc ggttcgacca tgccggcagc ggtgtggttc tcatactctc ccgaccgcaa
     1921 aggcatccac ccacagcaac atctggcgga ctacagaggt atcctgcagg ccgatgcata
     1981 tgcgggttac aatgctcttt acgaaagcgg tcaggtaacc gaagcggctt gtatggcaca
     2041 tgcccgacgc aagatccacg atgtacatgt ccgccatcca acgacagtaa cgggagaagc
     2101 gctccgtcgt atcggggaac tgtacgctat cgaggctgag atccgcggca gtccggcaga
     2161 agagcgactg gcggtcagaa aagccagaac ggtaccgcta atgcagtcgt tgtatgagtg
     2221 gctccagggg cagatgagca cgctgtcgcg ccactcggat acagcgaaag cgttcaccta
     2281 tctgctgaag caatgggacg ctctgaacga atactgccgc aatggctggg tggagatcga
     2341 caataacctg tgtgaaaacg ccctccgggt agttgcactg gggcggcgta actacatgtt
     2401 cttcggctct gatggtggag gcgagagtgc ggcagtgatg tacagcctga tcggtagctg
     2461 caaacttaac ggaatcgagc cggaaacgtg gctacgccac gtgatcagtg taatcaacac
     2521 ctggcctgcc aa
//
LOCUS       DAZKHF020000398         2498 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2201S31241
            SAMN43267800-rid25106413.denovo.400, whole genome shotgun sequence.
ACCESSION   DAZKHF020000398 DAZKHF020000000
VERSION     DAZKHF020000398.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267800
            Sequence Read Archive: SRR30307263
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2498)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2498)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 2498)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350581.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350581.2
            Genome Coverage       :: 151x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:21:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,652
            CDSs (total)                      :: 5,557
            Genes (coding)                    :: 5,320
            CDSs (with protein)               :: 5,320
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 237
            CDSs (without protein)            :: 237
            Pseudo Genes (ambiguous residues) :: 0 of 237
            Pseudo Genes (frameshifted)       :: 102 of 237
            Pseudo Genes (incomplete)         :: 130 of 237
            Pseudo Genes (internal stop)      :: 52 of 237
            Pseudo Genes (multiple problems)  :: 41 of 237
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2498
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267800-rid25106413.denovo.400"
                     /serotype="O 26:H11"
                     /isolate="2201S31241"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-01"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            complement(102..956)
                     /gene="rclR"
                     /locus_tag="ACHOSC_RS23770"
                     /old_locus_tag="pgaptmp_004754"
     CDS             complement(102..956)
                     /gene="rclR"
                     /locus_tag="ACHOSC_RS23770"
                     /old_locus_tag="pgaptmp_004754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="reactive chlorine-specific transcriptional
                     regulator RclR"
                     /protein_id="HGU4550528.1"
                     /translation="MDALSRLLMLNAPQGTIDKNCVLGSDWQLPHGAGELSVIRWHAL
                     TQGAAKLEMPTGEIFTLRPGNVVLLPQNSAHRLSHVDNESTCIVCGTLRLQHSARYFL
                     TSLPETLFLAPVNHSVEYNWLREAIPFLQQESRSAMPGVDALCSQICATFFTLAVREW
                     IAQVNTEKNILSLLLHPRLGAVIQQMLEMPGHAWTVESLASIAHMSRASFAQLFRDVS
                     GTTPLAVLTKLRLQIAAQMFSREMLPVVVIAESVGYASESSFHKAFVREFGCTPGEYR
                     ERVRQLAP"
     gene            1182..>2498
                     /gene="rclA"
                     /locus_tag="ACHOSC_RS23775"
                     /old_locus_tag="pgaptmp_004755"
     CDS             1182..>2498
                     /gene="rclA"
                     /locus_tag="ACHOSC_RS23775"
                     /old_locus_tag="pgaptmp_004755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414838.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="reactive chlorine resistance oxidoreductase
                     RclA"
                     /protein_id="HGU4550529.1"
                     /translation="MNKYQAVIIGFGKAGKTLAVTLAKAGWRVALIEQSNAMYGGTCI
                     NIGCIPTKTLVHDAQQHTDFVRAIQRKNEVVNFLRNKNFHNLADMPNIDVIDGQAEFI
                     NNHSLRVHRPEANLEIHGEKIFINTGAQTVVPPIPGITTTPGVYDSTGLLNLKELPGH
                     LGILGGGYIGVEFASMFANFGSKVTILEAASLFLPREDRDIADNIATILRDQGVDIIL
                     NAHVERISHHENQVQVHSEHAQLAVDALLIASGHQPATASLHPENAGIAVNERGAIVV
                     DKQLHTTADNIWAMGDVTGGLQFTYISLDDYRIVRDELLGEGRRSTDDRKNVPYSVFM
                     TPPLSRVGMTEEQARESGADIQVVTLPVAAIPRARVMNDTRGVLKAIVDNKTQRILGA
                     SLLCVDSHEMINIVKMVMDAGLPYSILRDQIFTHPSMSESLNDLFSL"
BASE COUNT          702 a          561 c          591 g          644 t
ORIGIN      
        1 aaatatataa agcccaaacc accgttggaa atagaaatag ttgaaaggct cacaactccc
       61 ggaatgcgga ttatctttgc cctgatttct ggcggtttta ttcagggtgc aagctgtctg
      121 accctttccc gatattctcc cggagtacaa ccaaactcgc ggacaaacgc cttgtgaaaa
      181 gatgattcac tggcatagcc tactgactca gcgatcacca caacagggag catttcccgg
      241 gaaaacatct gggccgctat ttgtagacgc aactttgtta atacagccag cggcgtggtt
      301 ccggaaacat cacggaaaag ctgggcaaaa cttgcccggg acatgtgagc gatgctggcc
      361 agcgattcga cggtccaggc gtgtcctggc atttccagca tttgctgtat taccgcacca
      421 agacgtggat gcagaagcaa actgagaatg tttttctcag tattaacctg tgcaatccac
      481 tcacgcaccg cgagggtaaa gaatgtagcg cagatctggc tacacagtgc atccactccc
      541 ggcatcgccg atctggattc ctgttgtaaa aacgggatcg cctccctcag ccagttatat
      601 tccacgctgt ggttaaccgg tgctaaaaac agcgtttccg gcaaagacgt taaaaaataa
      661 cgcgccgaat gttgcaaccg aagagtgccg cagacaatac aggtcgattc attatcgaca
      721 tgactcaggc gatgagctga attttgtggt agcaggacca catttcccgg gcgtaatgta
      781 aaaatctctc ccgtcggcat ttccagcttc gccgctcctt gcgttaacgc atgccaacga
      841 ataaccgata attccccggc accatgtgga agctgccagt cacttcctaa cacgcaattc
      901 ttatcgatcg ttccttgtgg agcgttaagc atcaacaaac ggctaagggc atccatatca
      961 gactcctgag tggtttttga ttacaaacac caacaatctt agtaaataac taatctaata
     1021 ccatcaaatg gttaatcatg atttttgttt tgtgattctg attaagaaaa agaaaatacc
     1081 agacgataag acaaaatatc aagcgttgtg aagaaatggt atttgctctt ttgcgtctat
     1141 agtcatgatg tcaaatgaac gcgtttcgac aggaaatcat catgaataaa tatcaggcag
     1201 tgattattgg ttttggcaag gctggaaaaa cattagccgt cacgctggca aaagcaggtt
     1261 ggcgagtggc tctcatcgaa caatcaaatg caatgtatgg cgggacctgt attaatatcg
     1321 gctgcatccc aaccaaaaca ttggttcatg acgcacagca gcacacagat tttgtccgtg
     1381 ccatacagcg taaaaatgaa gtggttaatt ttttacgtaa taagaatttt cataatcttg
     1441 cggatatgcc caatatcgac gtgatcgacg gccaggcgga gtttatcaat aatcatagcc
     1501 tgcgtgttca tcggcctgag gcaaatctgg aaattcatgg cgagaaaatt tttattaata
     1561 ccggtgcaca aaccgtggtt ccgccaattc ctggaattac caccacgcca ggagtatatg
     1621 acagcaccgg attacttaat ctaaaagaat tgcctgggca tttaggtatt ttgggcggcg
     1681 gatatattgg cgttgagttc gcctctatgt tcgctaattt tggcagcaag gtaaccattt
     1741 tagaagcagc ttcgctgttt ttgcctcggg aagatcggga tattgctgat aatatcgcga
     1801 cgattttacg cgatcagggc gtcgatatta tcctcaatgc ccatgtggag cgaatcagtc
     1861 accatgaaaa tcaagtgcaa gtgcatagcg agcacgccca actggcggtg gatgcactgt
     1921 taatagcttc cggtcatcaa ccggctaccg cttcgttaca tccagaaaat gccggtatcg
     1981 cagtaaacga gcgcggggca attgtcgttg acaagcaatt acataccacc gcagacaata
     2041 tttgggcgat gggagatgtt accggcgggc tgcaatttac ttatatttca ctggatgatt
     2101 accgcattgt gcgtgatgag ttgctgggag aaggcagacg tagtactgat gatcggaaaa
     2161 atgtgcctta ttccgtattc atgacaccgc cactgtccag ggttggtatg acagaagaac
     2221 aggccagaga gagtggtgct gatattcagg tggtgacatt gcctgtagct gcaattccgc
     2281 gtgccagagt gatgaatgat actcgtgggg tattaaaagc gattgttgat aataaaaccc
     2341 aacgtatatt aggggcatca ctgctgtgtg ttgactccca cgagatgatc aatatagtga
     2401 aaatggtgat ggatgccggg ctgccttata gcatattacg cgatcagata tttactcatc
     2461 cgtcgatgag cgaatcactc aatgatctat tttcatta
//
LOCUS       DAZKHF020000399         2481 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2201S31241
            SAMN43267800-rid25106413.denovo.401, whole genome shotgun sequence.
ACCESSION   DAZKHF020000399 DAZKHF020000000
VERSION     DAZKHF020000399.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267800
            Sequence Read Archive: SRR30307263
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2481)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2481)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 2481)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350581.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350581.2
            Genome Coverage       :: 151x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:21:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,652
            CDSs (total)                      :: 5,557
            Genes (coding)                    :: 5,320
            CDSs (with protein)               :: 5,320
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 237
            CDSs (without protein)            :: 237
            Pseudo Genes (ambiguous residues) :: 0 of 237
            Pseudo Genes (frameshifted)       :: 102 of 237
            Pseudo Genes (incomplete)         :: 130 of 237
            Pseudo Genes (internal stop)      :: 52 of 237
            Pseudo Genes (multiple problems)  :: 41 of 237
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2481
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267800-rid25106413.denovo.401"
                     /serotype="O 26:H11"
                     /isolate="2201S31241"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-01"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            109..2388
                     /gene="ydeP"
                     /locus_tag="ACHOSC_RS23780"
                     /old_locus_tag="pgaptmp_004756"
     CDS             109..2388
                     /gene="ydeP"
                     /locus_tag="ACHOSC_RS23780"
                     /old_locus_tag="pgaptmp_004756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid resistance putative oxidoreductase YdeP"
                     /protein_id="HGU4550530.1"
                     /translation="MKKKIESYQGAAGGWGAVKSVANAVRKQMDIRQDVIAMFDMNKP
                     EGFDCPGCAWPDPKHSASFDICENGAKAIAWEVTDKQVNASFFAENTVQSLLSWGDHE
                     LEAAGRLTQPLKYDAVSDCYKPLSWQQAFDEIGARLQSYSDPNQVEFYTSGRTSNEAA
                     FLYQLFAREYGSNNFPDCSNMCHEPTSVGLAASIGVGKGTVLLEDFEKCDLVICIGHN
                     PGTNHPRMLTSLRALVKRGAKMIAINPLQERGLERFTAPQNPFEMLTNSETQLASAYY
                     NVRIGGDMALLKWMMRLLIERDDAASAAGRPSLLDDEFIQTHTVGFDELRRDVLNSEW
                     KDIERISGLSQTQIAELADAYAAAERTIICYGMGITQHEHGTQNVQQLVNLLLMKGNI
                     GKPGAGICPLRGHSNVQGDRTVGITEKPSAEFLARLGERYGFTPPHAPGHAAIASMQA
                     ICTGQARALICMGGNFALAMPDREASAVPLTQLDLAVHVATKLNRSHLLTARHSYILP
                     VLGRSEIDMQKSGAQAVTVEDSMSMIHASRGVLKPAGVMLKSECAVVAGIAQATLPQS
                     VVAWEYLVEDYDRIRNDIEAVLPEFADYNQRIRHPGGFHLINAAAERRWMTPSGKANF
                     ITSKGLLEDPSSAFNSKLVMATVRSHDQYNTTIYGMDDRYRGVFGQRDVVFMSAKQAK
                     ICRVKNGERVNLIALTPDGKRSSRRMDRLKVVIYPMADRSLVTYFPESNHMLTLDNHD
                     PLSGIPGYKSIPVELEPSN"
BASE COUNT          637 a          590 c          629 g          625 t
ORIGIN      
        1 agtagtcccc tactcttata tatccatgtt ggcgataatc cctttgtatg tactgtgcat
       61 catcgctatt acaaatccta ataattcatt tccacacagg ataagtagat gaagaaaaaa
      121 attgaatcct accagggtgc tgcaggtggt tggggtgctg ttaaatccgt agcgaatgca
      181 gtacgtaagc agatggatat acgccaggat gttattgcca tgtttgacat gaataagcca
      241 gagggctttg actgtccggg ttgtgcatgg ccagatccta agcacagtgc gtcattcgac
      301 atttgtgaaa acggcgcaaa agcaatcgcc tgggaagtca cggataagca ggtaaatgcc
      361 tctttctttg ctgagaatac ggttcaatca ttactttcct ggggagatca cgagcttgag
      421 gctgcgggac gtctcactca gcctttgaaa tatgatgccg tcagcgactg ttacaagcca
      481 ttaagctggc aacaagcttt cgacgaaatt ggcgcacgcc ttcaaagcta tagtgatccc
      541 aatcaggttg aattctatac ttcgggccgc acttccaatg aagctgcctt tctttatcag
      601 ctttttgccc gtgaatacgg gagcaataac tttcccgact gctccaacat gtgccatgaa
      661 ccgacaagcg tgggtttggc agcgagtatc ggtgtaggta aagggaccgt gttgctggaa
      721 gactttgaga agtgcgattt agtcatttgc attgggcata accctggtac aaaccaccct
      781 cgcatgctga cttcgttgcg cgctttagtg aaacggggag cgaaaatgat cgccatcaat
      841 cctctacagg aacgtggcct ggagcgattt accgcaccgc aaaacccgtt tgaaatgctg
      901 acgaactctg agactcagtt ggccagtgcc tactacaacg tgcgcattgg tggcgatatg
      961 gcgttgctca agtggatgat gcgcctgtta attgagcgcg atgatgctgc aagtgccgca
     1021 ggtcggccct cattgcttga tgacgaattt attcaaacgc ataccgtcgg ctttgacgag
     1081 ctacgccgtg acgttctcaa ttccgagtgg aaagatatcg aacgtatttc tggactaagt
     1141 cagacacaaa tcgccgaact ggctgacgca tatgccgctg ccgaacgcac cattatctgt
     1201 tacggaatgg ggatcactca gcacgaacat ggtacccaga acgtacagca actggtcaat
     1261 ttgctgttga tgaaaggtaa cattggcaag cctggtgcgg gtatctgccc actacgtggt
     1321 cactctaatg tacagggcga ccgaaccgtc ggtatcaccg agaaaccgtc tgcagagttt
     1381 ctggctcgcc tgggtgagcg ctatggcttc accccacctc atgcacctgg acatgctgca
     1441 attgccagca tgcaagcaat atgtacgggg caggctcgag cattgatctg catggggggc
     1501 aactttgcac tggcaatgcc agatcgggaa gcgagcgctg taccgttaac gcaattagat
     1561 ttggcggtac acgtagccac taagcttaac cgctctcatc tgctgaccgc acggcatagc
     1621 tatatcctgc cggttctggg gcgtagcgag attgatatgc aaaaaagcgg tgcgcaggcg
     1681 gtaaccgttg aggattcaat gtcgatgatt catgcctcgc gtggcgtgtt aaaacccgcc
     1741 ggtgtaatgc tgaaatcaga gtgtgctgtg gtcgcgggaa tcgcccaggc aacactaccc
     1801 cagagcgtgg tagcctggga gtatctggtg gaagattatg atcgcattcg caatgacatt
     1861 gaagctgtgc tgccagagtt cgccgactat aaccagcgca tccgtcatcc cggtggtttt
     1921 cacctgataa atgcagctgc tgaaaggcgc tggatgacgc cgtcaggtaa ggctaatttc
     1981 attaccagca aagggctgtt agaagatccc tcttcagcgt ttaacagtaa gctggtcatg
     2041 gcgacagtac gcagccacga tcagtacaac acgacgattt atggtatgga tgatcgctat
     2101 cgaggggtat tcggtcaacg agatgtggtc tttatgagtg ctaaacaagc taaaatttgc
     2161 cgtgtaaaaa acggcgaaag agttaatctt attgcgctta cgccagacgg taagcgcagc
     2221 tcacgccgca tggatagatt aaaagtggtc atttacccta tggctgaccg ctcactggtg
     2281 acctattttc cagaatcgaa tcacatgcta actcttgata accacgatcc attaagtggc
     2341 attcctggtt ataaaagtat tccggttgaa ttagaaccat caaattaatt cgcctcctca
     2401 tttcttctgc tgtcatccgc acagcagaag aatttctcat tgactattat cgcgcaattt
     2461 gctcacattg attaaattaa a
//
LOCUS       DAZKHF020000400         2471 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2201S31241
            SAMN43267800-rid25106413.denovo.402, whole genome shotgun sequence.
ACCESSION   DAZKHF020000400 DAZKHF020000000
VERSION     DAZKHF020000400.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267800
            Sequence Read Archive: SRR30307263
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2471)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2471)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 2471)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350581.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350581.2
            Genome Coverage       :: 151x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:21:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,652
            CDSs (total)                      :: 5,557
            Genes (coding)                    :: 5,320
            CDSs (with protein)               :: 5,320
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 237
            CDSs (without protein)            :: 237
            Pseudo Genes (ambiguous residues) :: 0 of 237
            Pseudo Genes (frameshifted)       :: 102 of 237
            Pseudo Genes (incomplete)         :: 130 of 237
            Pseudo Genes (internal stop)      :: 52 of 237
            Pseudo Genes (multiple problems)  :: 41 of 237
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2471
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267800-rid25106413.denovo.402"
                     /serotype="O 26:H11"
                     /isolate="2201S31241"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-01"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            130..318
                     /locus_tag="ACHOSC_RS23785"
                     /old_locus_tag="pgaptmp_004757"
     CDS             130..318
                     /locus_tag="ACHOSC_RS23785"
                     /old_locus_tag="pgaptmp_004757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001352456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HGU4550531.1"
                     /translation="MLSTDRFIREKECQQLTGLSRSCRYRLEKAGQFPSRRKLGGRSV
                     GWSLSEVLAWKDSCEAVH"
     gene            complement(1078..1257)
                     /locus_tag="ACHOSC_RS23790"
                     /old_locus_tag="pgaptmp_004758"
     CDS             complement(1078..1257)
                     /locus_tag="ACHOSC_RS23790"
                     /old_locus_tag="pgaptmp_004758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000190566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlcI/YnfO family protein"
                     /protein_id="HGU4550532.1"
                     /translation="MATGSTNNKSQQLNARFPHDVVADLEKNLDEGESKAQFIVTAVK
                     GEIKRRQRRKAKEQE"
     gene            1448..1648
                     /locus_tag="ACHOSC_RS23795"
                     /old_locus_tag="pgaptmp_004759"
     CDS             1448..1648
                     /locus_tag="ACHOSC_RS23795"
                     /old_locus_tag="pgaptmp_004759"
                     /inference="COORDINATES: protein motif:HMM:NF033153.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="host cell division inhibitor Icd-like protein"
                     /protein_id="HGU4550533.1"
                     /translation="MRNHTIHPQGRDSHNLNKYIWRFIALSTAQPRVITIEATSEQEA
                     RQQSPAGCVMVFAARIRQGVGL"
     gene            1645..1875
                     /locus_tag="ACHOSC_RS23800"
                     /old_locus_tag="pgaptmp_004760"
     CDS             1645..1875
                     /locus_tag="ACHOSC_RS23800"
                     /old_locus_tag="pgaptmp_004760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000076676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4550534.1"
                     /translation="MSQEITLQQAAERAHQIEVICALAEDYPGMMTDSESGAIIGLLK
                     RLSGEVCVFLSDEQERRTLISNEKKCGGVHVQ"
     gene            1865..2089
                     /locus_tag="ACHOSC_RS23805"
                     /old_locus_tag="pgaptmp_004761"
     CDS             1865..2089
                     /locus_tag="ACHOSC_RS23805"
                     /old_locus_tag="pgaptmp_004761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000336139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4550535.1"
                     /translation="MCNKTTPDAAAAALTTLMHALIDISVIADRAHKHAARESECIFH
                     YLTFVQLKADQALDKAGKIIMADVQEVHHA"
     gene            2082..2447
                     /locus_tag="ACHOSC_RS23810"
                     /old_locus_tag="pgaptmp_004762"
     CDS             2082..2447
                     /locus_tag="ACHOSC_RS23810"
                     /old_locus_tag="pgaptmp_004762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001723121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4550536.1"
                     /translation="MHNLSISDLNSIQFDEKFTGQLLVNVENGRIVRNYHLPDGAIAG
                     SVEALLELAERARLIKPSTSHHDDDLHFTGCMVSHYENGVEVSCERLRDDCCFGTLPE
                     FIELLTSCGYQVIQGGKHA"
BASE COUNT          675 a          612 c          590 g          594 t
ORIGIN      
        1 cattccccct gcttctgtaa aacgaccgcc gccagtgttt gccattccac aaacaccctg
       61 acacaatccg acacaaccca acccaataat ccacccaatg actctttaag aaacaacaaa
      121 gggggaattg tgttaagcac agatcgcttt atacgtgaaa aagaatgtca gcaacttaca
      181 ggcctgagcc gctcatgtcg gtaccgcctg gaaaaagccg gacaattccc atcacggcgt
      241 aagctgggcg gtcgttccgt tggctggtct ttatccgaag ttctggcatg gaaggacagc
      301 tgcgaggcag ttcactaatc aggctggcgg cacacagccg ccacccatcc actaatacag
      361 agatctaacc atgaatactg gatattctcc cgaacaaggg cggggcttcg ttcgtcctga
      421 aaaacagaat ctgcaaaatt ttgccgaaat tattccggtt atttccggcc ttactggcgg
      481 gagtgaaacc aatattgtta acgccagagc gttgcagatg tttgatgata aaaagggagt
      541 aaatttaact tacacccctg acggcaatca gaatatgagc attatctctg agtcaggttt
      601 ctacaaacta ataaaaacaa aaagcgcccc gttgccggag cgcctttgtg aacaattaac
      661 ctactgcgca aaaaatgaat ctgagcagtg ggattatatc aaccatgtgg agaagcgcca
      721 caattgccga ataacgggca aaacaaaggc cacccgctac ggtggcccct cgacacaagc
      781 tacacgttat ccccaacgca tgagcattgc caacaatgcc acatttgcgg ctggtgggca
      841 atgcaatcag tctggttcag ttcgttgcca tacctgcaat gagcgctttt ccctgtactc
      901 tttaaggaat tgctcaaggg caaaagcaca tggcgcgaat ctttctgatt catgctctat
      961 ctttctgcgc cgtcttttcc gtgccggtga taatgttttg gtcaattctt tatcggtcat
     1021 tgtgttgtcc tgcatagcaa tgcgccgtag ttactcacac cacggcgctg gtgatggtta
     1081 ctcctgctct ttggccttgc gacgctggcg gcgtttgatc tcacctttaa cggcagtaac
     1141 tataaattgc gccttgcttt caccttcatc taaatttttt tctaagtctg caacaacatc
     1201 atgcgggaat ctggcattta actgttgcga cttattattt gttgaacctg ttgccattac
     1261 tgaatcctct ctaagttggt gcgattcagt atacacaaaa aaaatttcag agaaagactt
     1321 gaagtgcgat tcacttactg ataggttaaa aacgaatggt gcgatgcacc aaacaacaac
     1381 gccccgcagt ggtggcacac atgcagggcg tctaaccacc aacgatagcg aaagtatcga
     1441 ggtagctatg agaaatcata ccatacaccc gcaagggcgg gactcgcaca acctgaataa
     1501 atacatctgg cgttttatcg ccctgagcac ggcacaaccg cgcgtgatta ccattgaggc
     1561 caccagcgaa caggaagcac gccagcaatc tccggctggc tgcgtgatgg tatttgctgc
     1621 ccgtattcgt cagggggtag gcttatgagc caggaaatca cactacaaca ggcagcagaa
     1681 cgcgcccacc aaatcgaagt tatttgcgca ctggcagagg attaccctgg catgatgacc
     1741 gacagcgaat caggggcaat catcggctta cttaaacgcc ttagcggtga ggtctgcgta
     1801 ttcctgagcg atgaacagga aagaagaacg cttatttcta acgaaaaaaa atgcggaggg
     1861 gtacatgtgc aataaaacca caccggacgc agccgccgcc gcgctcacta cgctgatgca
     1921 cgcgcttatt gatatttctg ttattgctga cagggcgcat aaacacgcag ccagagaatc
     1981 agaatgcatc ttccattatt taacatttgt gcagctaaaa gccgatcagg cactggataa
     2041 ggccggaaaa attatcatgg ctgatgtgca ggaggtgcac catgcataac ctgtcaattt
     2101 ctgaccttaa cagcattcag tttgacgaga aatttaccgg gcagttgctg gtcaatgtgg
     2161 agaacgggcg catagtgcgt aattatcacc tgccggatgg tgcaattgcc ggaagcgttg
     2221 aagcattgct ggaactggcg gaacgtgcgc gactgattaa gccgtcaacg agccatcacg
     2281 atgatgatct gcattttacc ggatgtatgg tgagtcacta cgaaaacggc gtcgaggtat
     2341 cctgcgaacg gctgcgtgat gattgctgtt tcggcacact gccggaattt atcgagttgc
     2401 tgaccagttg cggttatcag gtcattcagg ggggtaaaca tgcgtgatga tcgttttaat
     2461 tccctgaaac a
//
LOCUS       DAZKHF020000401         2411 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2201S31241
            SAMN43267800-rid25106413.denovo.403, whole genome shotgun sequence.
ACCESSION   DAZKHF020000401 DAZKHF020000000
VERSION     DAZKHF020000401.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267800
            Sequence Read Archive: SRR30307263
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2411)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2411)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 2411)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350581.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350581.2
            Genome Coverage       :: 151x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:21:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,652
            CDSs (total)                      :: 5,557
            Genes (coding)                    :: 5,320
            CDSs (with protein)               :: 5,320
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 237
            CDSs (without protein)            :: 237
            Pseudo Genes (ambiguous residues) :: 0 of 237
            Pseudo Genes (frameshifted)       :: 102 of 237
            Pseudo Genes (incomplete)         :: 130 of 237
            Pseudo Genes (internal stop)      :: 52 of 237
            Pseudo Genes (multiple problems)  :: 41 of 237
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2411
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267800-rid25106413.denovo.403"
                     /serotype="O 26:H11"
                     /isolate="2201S31241"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-01"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            268..411
                     /locus_tag="ACHOSC_RS23815"
                     /old_locus_tag="pgaptmp_004763"
     CDS             268..411
                     /locus_tag="ACHOSC_RS23815"
                     /old_locus_tag="pgaptmp_004763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2627 domain-containing protein"
                     /protein_id="HGU4550537.1"
                     /translation="MCGIFSKEVLSKHVDVEYRFSAEPYIGASCSNVSVLSMLCLRAK
                     KTI"
     gene            424..633
                     /gene="cspE"
                     /locus_tag="ACHOSC_RS23820"
                     /old_locus_tag="pgaptmp_004764"
                     /gene_synonym="gicA"
                     /gene_synonym="msmC"
     CDS             424..633
                     /gene="cspE"
                     /locus_tag="ACHOSC_RS23820"
                     /old_locus_tag="pgaptmp_004764"
                     /gene_synonym="gicA"
                     /gene_synonym="msmC"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator/RNA stability
                     regulator CspE"
                     /protein_id="HGU4550538.1"
                     /translation="MAKIKGQVKWFNESKGFGFITPADGSKDVFVHFSAIQGNGFKTL
                     AEGQNVEFEIQDGQKGPAAVNVTAI"
     gene            799..1608
                     /gene="rlmA"
                     /locus_tag="ACHOSC_RS23825"
                     /old_locus_tag="pgaptmp_004765"
     CDS             799..1608
                     /gene="rlmA"
                     /locus_tag="ACHOSC_RS23825"
                     /old_locus_tag="pgaptmp_004765"
                     /EC_number="2.1.1.187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (guanine(745)-N(1))-methyltransferase"
                     /protein_id="HGU4550539.1"
                     /translation="MSFSCPLCHQPLSREKNSYICPQRHQFDMAKEGYVNLLPVQHKR
                     SRDPGDSAEMIQARRAFLDAGHYQPLRDAIVAQLRERLDEKATAVLDIGCGEGYYTHA
                     FADVLPEITTFGLDVSKVAIKAAAKRYPQVTLCVASSHRLPFSDTSMDAIIRIYAPCK
                     AEELARVVKPGGWVITATPGPRHLMELKGLIYNEVHLHAPHAEQLEGFTLQQSDELCY
                     PMRLRGDEAVALLQMTPFAWRAKPEVWQTLAAKEVFDCQTDFNIHLWQRSY"
     gene            complement(1605..2171)
                     /gene="mntP"
                     /locus_tag="ACHOSC_RS23830"
                     /old_locus_tag="pgaptmp_004766"
     CDS             complement(1605..2171)
                     /gene="mntP"
                     /locus_tag="ACHOSC_RS23830"
                     /old_locus_tag="pgaptmp_004766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416335.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="manganese efflux pump MntP"
                     /protein_id="HGU4550540.1"
                     /translation="MNITATVLLAFGMSMDAFAASIGKGATLHKPKFSEALRTGLIFG
                     AVETLTPLIGWGMGMLASRFVLEWNHWIAFVLLIFLGGRMIIEGFRGADDEDEEPRRR
                     HGFWLLVTTAIATSLDAMAVGVGLAFLQVNIIATALAIGCATLIMSTLGMMVGRFIGS
                     IIGKKAEILGGLVLIGIGVQILWTHFHG"
BASE COUNT          632 a          587 c          593 g          599 t
ORIGIN      
        1 gcactatgta ataacttctc ccactggcct ggaacaactg aacttattga actatgttag
       61 aaaatacgcc agtttaagta tctgcctgaa ctggcaaggt taagcacaat gatatatcgg
      121 cgcgtattcc gttgcataag tgtgcaaaaa aagtggaaga cgtatcgaga tttgtgcgtc
      181 tgatcgagac atgtttaaaa atggcttgcc ataattaacg ttgtatgtga taacagattt
      241 cgggttaaac gaggtacagt tctgtttatg tgtggcattt tcagtaaaga agtcctgagt
      301 aaacacgttg acgttgaata ccgcttctct gccgagcctt atattggtgc ctcatgcagt
      361 aatgtgtcag ttttatctat gttatgcctg cgggcgaaga aaacaatcta aggaattttt
      421 caaatggcaa agattaaagg tcaggttaag tggttcaacg agtctaaagg ttttggcttc
      481 attactccgg ctgatggcag caaagatgtg ttcgtacact tctccgctat ccagggtaat
      541 ggcttcaaaa ctctggctga aggtcagaac gttgagttcg aaattcagga cggccagaaa
      601 ggtccggcag ctgttaacgt aacagctatc tgatcgaatc cactgatctg aagtgtgaat
      661 acgcttcaat ctcgctataa agcctcgtcg aatgcgaggc tttttactat gctttatctt
      721 cgctcctggc gttcggatat ttgcccgccg cgtgattcgc gttacacttg cggcctttag
      781 tatcctgccg gagttgtcat gtcgttttcc tgtccacttt gccatcagcc tctttcgcgt
      841 gaaaaaaaca gctatatctg tccccagcga catcagtttg atatggcgaa agaagggtat
      901 gtcaatctgc tgcccgttca gcataaacgg tctcgtgatc cgggcgacag cgcggaaatg
      961 atacaagcac gccgcgcatt cttagatgcc ggacattatc agccgctgcg tgatgcaatt
     1021 gtcgcccaac tgcgggaacg gcttgatgaa aaggccacgg cggtgctgga tattggctgt
     1081 ggtgaagggt attacacaca cgcatttgcc gatgtgttgc ccgaaatcac cacgtttggc
     1141 ctggatgtct cgaaggtagc gataaaagcg gcggcgaaac gctatccgca ggtcactctt
     1201 tgtgtcgctt ccagccaccg tttgccgttt tccgatacca gtatggacgc cataatacgt
     1261 atttacgcgc cgtgtaaagc agaagaatta gcacgagtag tgaagcccgg cggctgggtc
     1321 attactgcca cgccgggacc gcgacatttg atggagctga aggggctgat ttacaatgaa
     1381 gtacatcttc atgcacctca tgcagaacaa ctggaaggtt ttacattaca gcagagtgat
     1441 gagttgtgtt atccgatgcg tcttcgcggt gatgaagccg tcgcattatt gcagatgacg
     1501 ccgtttgcct ggcgtgcgaa gccagaagtc tggcaaacac tggcagcaaa agaagtgttc
     1561 gactgccaga cggactttaa tattcacctc tggcagcgtt cttattaacc gtggaagtgc
     1621 gtccagagga tctggacgcc gatgccgatc agcaccagcc cgccgagaat ttccgctttt
     1681 ttcccaataa ttgagccgat aaagcgacca accatcatcc ctaatgtcga cataatcaag
     1741 gttgcacaac caatggccaa tgcggtcgcg ataatgttga cctgcaggaa agcaagacca
     1801 acacccacag ccatggcatc caggctggtg gcaatcgcgg tggttaccag tagccagaaa
     1861 ccgtgccgac ggcgcggctc ttcatcttca tcatctgcgc cacgaaaacc ctcaataatc
     1921 attcgcccgc cgaggaatat cagcagcaca aacgcaatcc agtggttcca ttcaaggaca
     1981 aaccggctgg ctaacatgcc cattccccag ccgatcagcg gcgtcagggt ttcgacggca
     2041 ccaaaaataa ggccggttcg caatgcttca gaaaatttcg gtttatggag ggtggcacct
     2101 ttaccgattg atgcagcaaa tgcatccatc gacataccaa acgcaagaag aacagtagca
     2161 gtgatattca tgacaatgtc ctgaccgggg tatccataaa acacatcatc gcccccagca
     2221 aacatgcata catgaacaac aagccgcgat ggttttcgcg ttcatgcagg catagcgatt
     2281 gatgtgcata tggtctcgcc tgactgaaag tattcagtcc gtacgtgcca cgttttgcaa
     2341 cgagtatgtt gacacgtaca tttccggaat ctggaaatcg gctactcccc aaatgagggc
     2401 gcaaccttaa c
//
LOCUS       DAZKHF020000402         2389 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2201S31241
            SAMN43267800-rid25106413.denovo.404, whole genome shotgun sequence.
ACCESSION   DAZKHF020000402 DAZKHF020000000
VERSION     DAZKHF020000402.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267800
            Sequence Read Archive: SRR30307263
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2389)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2389)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 2389)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350581.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350581.2
            Genome Coverage       :: 151x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:21:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,652
            CDSs (total)                      :: 5,557
            Genes (coding)                    :: 5,320
            CDSs (with protein)               :: 5,320
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 237
            CDSs (without protein)            :: 237
            Pseudo Genes (ambiguous residues) :: 0 of 237
            Pseudo Genes (frameshifted)       :: 102 of 237
            Pseudo Genes (incomplete)         :: 130 of 237
            Pseudo Genes (internal stop)      :: 52 of 237
            Pseudo Genes (multiple problems)  :: 41 of 237
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2389
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267800-rid25106413.denovo.404"
                     /serotype="O 26:H11"
                     /isolate="2201S31241"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-01"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            48..536
                     /gene="eco"
                     /locus_tag="ACHOSC_RS23835"
                     /old_locus_tag="pgaptmp_004767"
     CDS             48..536
                     /gene="eco"
                     /locus_tag="ACHOSC_RS23835"
                     /old_locus_tag="pgaptmp_004767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004867 - serine-type endopeptidase
                     inhibitor activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine protease inhibitor ecotin"
                     /protein_id="HGU4550541.1"
                     /translation="MKTILPAVLFAAFATTSAWAAESVQPLEKIAPYPQAEKGMKRQV
                     IQLTPQEDESTLKVELLIGQTLEVDCNLHRLGGKLESKTLEGWGYDYYVFDKVSSPVS
                     TMMACPDGKKEKKFVTAYLGDAGMLRYNSKLPIVVYTPDNVDVKYRVWKAEEKIDNAV
                     VR"
     gene            complement(685..2331)
                     /gene="mqo"
                     /locus_tag="ACHOSC_RS23840"
                     /old_locus_tag="pgaptmp_004768"
     CDS             complement(685..2331)
                     /gene="mqo"
                     /locus_tag="ACHOSC_RS23840"
                     /old_locus_tag="pgaptmp_004768"
                     /EC_number="1.1.5.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008924 - L-malate dehydrogenase (quinone)
                     activity [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malate dehydrogenase (quinone)"
                     /protein_id="HGU4550542.1"
                     /translation="MKKVTAMLFSMAVGLNAVSMAAKAKASEEQETDVLLIGGGIMSA
                     TLGTYLRELEPEWSMTMVERLEGVAQESSNGWNNAGTGHSALMELNYTPQNADGSISI
                     EKAVAINEAFQISRQFWAHQVERGVLRTPRSFINTVPHMSFVWGEDNVNFLRARYAAL
                     QQSSLFRGMRYSEDHAQIKEWAPLVMEGRDPQQKVAATRTEIGTDVNYGEITRQLIAS
                     LQKKSNFSLQLSSEVRALKRNDDNTWTVTVADLKNGTAQNIRAKFVFIGAGGAALKLL
                     QESGIPEAKDYAGFPVGGQFLVSENPDVVNHHLAKVYGKASVGAPPMSVPHIDTRVLD
                     GKRVVLFGPFATFSTKFLKNGSLWDLMSSTTTSNVMPMMHVGLDNFDLVKYLVSQVML
                     SEEDRFEALKEYYPQAKKEDWRLWQAGQRVQIIKRDAEKGGVLRLGTEVVSDQQGTIA
                     ALLGASPGASTAAPIMLDLLEKVFGDRVSSPQWQATLKAIVPSYGRKLNGDVAATERE
                     LQYTSEVLGLKYDKPQAADSTPKPQLKPQPVQKEVADIAL"
BASE COUNT          556 a          662 c          602 g          569 t
ORIGIN      
        1 cgacatcatc ggtgaaaacc tataaattga agaaggaaag caaaaaaatg aagaccattc
       61 tacctgcagt attgtttgcc gctttcgcta ccacttccgc ctgggcggca gaaagcgtcc
      121 agccactgga aaaaatcgcg ccttatccac aagctgaaaa agggatgaag cgtcaggtga
      181 ttcagttaac cccgcaagaa gatgaatcta ccctgaaagt agaactgtta atcggtcaga
      241 cgctggaagt cgattgcaat ttgcatcgtc tcggcgggaa gctggaaagc aaaacgctgg
      301 aaggctgggg ctatgattat tatgtctttg ataaagttag ctcgccggtt tccacgatga
      361 tggcctgccc ggatggcaag aaagagaaga aatttgtcac cgcgtatctg ggcgatgctg
      421 gaatgctgcg ttacaacagc aagctgccga tcgtggtgta tacgccagac aatgtagatg
      481 tgaagtaccg cgtctggaag gcggaagaga aaattgacaa cgcggtagtt cgctaaaatg
      541 ctgtgaagtg cggcaccccg taggccgaat aaggcggtca cgccgcatcc gacatctaac
      601 gcccgagccg gtgcctgatg cgacgctggc gcgtcttatc agcatacgcc acatccggca
      661 taccatgccg gatgtggcac atcattacaa cgcaatatcc gccacttctt tttgaacggg
      721 ttgcggtttc aactgcggtt ttggcgtact atctgctgcc tgcggcttgt cgtatttcag
      781 ccccagcact tcgctggtgt actgcaactc gcgttctgtt gccgctacat caccgttcag
      841 cttgcgtcca taagacggaa cgatcgcttt caacgtagcc tgccattgcg ggctggaaac
      901 gcgatcgcca aatacttttt ccagcagatc caacataatc ggcgcggcgg ttgacgcccc
      961 tggcgatgcc cccagcagcg cggcaatggt cccttgctgg tcactgacca cttcagtacc
     1021 cagacgcagt acgccacctt tctcggcatc acgcttgata atctgcacgc gctgccccgc
     1081 ttgccacaaa cgccagtcct ctttttttgc ctgcggatag tactctttca acgcttcaaa
     1141 acgatcctct tcactcaaca tcacctgact caccagatat ttcaccagat cgaaattatc
     1201 cagcccgacg tgcatcatcg gcatcacgtt agaggtggtg gtggaactca tcagatccca
     1261 caatgaaccg tttttgagga atttggttga gaaggtggca aatggcccaa acagcactac
     1321 gcgtttaccg tccagaacgc gggtatcgat atgcggaacc gacatcggtg gtgcgccaac
     1381 ggatgcttta ccgtaaacct tcgccagatg gtgattaacc acgtccgggt tttccgaaac
     1441 aaggaactgt ccgcccaccg ggaaaccggc gtagtctttc gcttccggaa tccccgattc
     1501 ctgtaatagc ttcagcgccg caccgcccgc gccgataaag acaaatttgg cgcggatgtt
     1561 ctgtgcagtg ccgtttttca gatcggcaac ggtaacggtc caggtgttat cgtcattacg
     1621 ctttagggcg cggacttcgc tgctgagttg cagcgagaag ttagatttct tctgtaagga
     1681 agcaattaac tggcgggtga tctcgccata gttcacatca gtgccaattt ctgtgcgtgt
     1741 tgccgctact ttctgttgtg gatcgcgccc ttccatcact aacggtgccc actctttgat
     1801 ctgcgcgtga tcttcagagt aacgcatacc gcgaaacagc gagctttgtt gcaacgcggc
     1861 gtaacgggcg cgcaggaaat taacgttatc ctcgccccag acaaagctca tatgcggaac
     1921 ggtattgata aatgaacgcg gagtacgcag cacgccgcgc tcaacctggt gcgcccagaa
     1981 ctggcgggaa atctgaaatg cttcgttaat ggcgactgct ttttcaatac tgatgctgcc
     2041 atcggcgttt tgcggggtgt agttcagttc catcagtgca gaatgcccgg ttccggcgtt
     2101 attccagccg ttcgaactct cctgcgcgac accctccagg cgctccacca tggtcatcga
     2161 ccattcaggc tccagctcgc gtaaataggt ccccaacgtg gcgctcataa tgccgccgcc
     2221 aatcaacagt acatcagttt cctgctcctc ggacgctttc gcttttgccg ccatcgaaac
     2281 ggcattaagc cccacggcca tcgagaagag catggcagtc acttttttca tcttgttaat
     2341 gccttacttt tagtcgcttt attgcaggtg agatttgcgc ggcatcaac
//
LOCUS       DAZKHF020000403         2369 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2201S31241
            SAMN43267800-rid25106413.denovo.405, whole genome shotgun sequence.
ACCESSION   DAZKHF020000403 DAZKHF020000000
VERSION     DAZKHF020000403.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267800
            Sequence Read Archive: SRR30307263
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2369)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2369)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 2369)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350581.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350581.2
            Genome Coverage       :: 151x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:21:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,652
            CDSs (total)                      :: 5,557
            Genes (coding)                    :: 5,320
            CDSs (with protein)               :: 5,320
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 237
            CDSs (without protein)            :: 237
            Pseudo Genes (ambiguous residues) :: 0 of 237
            Pseudo Genes (frameshifted)       :: 102 of 237
            Pseudo Genes (incomplete)         :: 130 of 237
            Pseudo Genes (internal stop)      :: 52 of 237
            Pseudo Genes (multiple problems)  :: 41 of 237
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2369
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267800-rid25106413.denovo.405"
                     /serotype="O 26:H11"
                     /isolate="2201S31241"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-01"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            104..796
                     /locus_tag="ACHOSC_RS23845"
                     /old_locus_tag="pgaptmp_004769"
     CDS             104..796
                     /locus_tag="ACHOSC_RS23845"
                     /old_locus_tag="pgaptmp_004769"
                     /EC_number="2.7.4.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004798 - thymidylate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006233 - dTDP biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0006235 - dTTP biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTMP kinase"
                     /protein_id="HGU4550543.1"
                     /translation="MQSITPPLIAVIGSDGSGKSTVCEHLITVVEKYGAAERVHLGKQ
                     AGNVGRAVTKLPLMGKSLHKTIERNQVKTAKKLPGPVPALVITAFVARRLLRFRHMLA
                     CRRRGLIVLTDRYPQDQIPGAYDGTVFPPNVEGGRFVSWLASQERKAFHWMASHKPDL
                     VIKLNVDLEVACARKPDHKRESLARKIAITPQLTFGGAQLVDIDANQPLEQVLVDAEK
                     AIMDFMTARGYH"
     gene            complement(845..2344)
                     /gene="pitB"
                     /locus_tag="ACHOSC_RS23850"
                     /old_locus_tag="pgaptmp_004770"
     CDS             complement(845..2344)
                     /gene="pitB"
                     /locus_tag="ACHOSC_RS23850"
                     /old_locus_tag="pgaptmp_004770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic phosphate transporter PitB"
                     /protein_id="HGU4550544.1"
                     /translation="MLNLFVGLDIYTGLLLLLALAFVLFYEAINGFHDTANAVATVIY
                     TRAMQPQLAVVMAAFFNFFGVLLGGLSVAYAIVHMLPTDLLLNMGSTHGLAMVFSMLL
                     AAIIWNLGTWFFGLPASSSHTLIGAIIGIGLTNALLTGSSVMDALNLREVTKIFSSLI
                     VSPIVGLVIAGGLIFLLRRFWSGTKKRDRIHRIPEDRKKKKGKRKPPFWTRIALIVSA
                     AGVAFSHGANDGQKGIGLVMLVLVGIAPAGFVVNMNASGYEITRTRDAVSNFEHYLQQ
                     HPELPQKLIAMEPPLPAASTDGAQVTEFHCHPANTFDAIARVKTMLPGNMESYEPLSV
                     SQRSQLRRIMLCISDTSAKLAKLPGVSKEDQNLLKKLRSDMLSTIEYAPVWIIMAVAL
                     ALGIGTMIGWRRVAMTIGEKIGKRGMTYAQGMAAQMTAAVSIGLASYIGMPVSTTHVL
                     SSAVAGTMVVDGGGLQRKTVTSILMAWVFTLPAAILLSGGLYWIALQLI"
BASE COUNT          645 a          604 c          568 g          552 t
ORIGIN      
        1 tgtgacaatg gttaattgtg acattgacct gaattacgct ttcataaaaa catattaacc
       61 aaataaatat tttggatgga tatttaaatt taaggatata ttcatgcagt caataacacc
      121 tccattaatt gccgttattg gtagcgatgg ttcaggcaag tcaacggtgt gtgaacatct
      181 tattaccgtt gtcgaaaaat atggtgctgc cgaaagagtt catttaggaa aacaagccgg
      241 aaatgtcggt cgtgcagtga caaaattacc gttgatggga aaatccttac ataaaacaat
      301 tgaacgaaat caggtgaaaa cagcaaaaaa attgccagga ccagttccgg cgctggtaat
      361 tacagcgttt gtcgcccgtc gcttactgcg ctttcgtcat atgcttgcct gtcgtcgtcg
      421 cgggttaatt gttctaaccg accgttatcc tcaggaccaa attcctggcg cttacgatgg
      481 tacggtgttc ccacctaacg ttgaaggtgg tcgttttgtc tcatggctgg caagccagga
      541 acgtaaagcg tttcactgga tggcgagcca taagcctgat ctggtcatca aactcaatgt
      601 tgaccttgaa gttgcctgcg cgcgtaagcc tgaccacaaa agggagtcgc tggcaagaaa
      661 gatagccata acgccacagt taacctttgg tggtgcacaa ctggttgata tcgatgccaa
      721 tcagccactg gaacaggtgt tggttgatgc agaaaaagcg attatggatt ttatgaccgc
      781 gcgtggttat cactagccaa aaatggaaat gcccgatcgc caggaccggg cattttcagg
      841 aaggttaaat caactgcaat gctatccagt acagcccacc agaaagcaaa atcgccgccg
      901 gtaaagtaaa tacccacgcc atcaggatgc tggttaccgt tttacgctgt aacccaccgc
      961 cgtccaccac catcgtccct gcaactgcag acgagaggac gtgtgttgtg gagacgggca
     1021 tcccaatata actggcaaga ccgatagaca ctgccgccgt catttgtgcc gccatgcctt
     1081 gcgcatacgt catgccgcgc ttaccaatct tctcaccgat ggtcatcgct acacgacgcc
     1141 agccaatcat ggtgccaatg ccgagcgcca gtgctaccgc catgatgatc cacaccggag
     1201 cgtactcaat ggtgcttaac atatcgctgc gcagtttttt cagcaggttc tggtcttctt
     1261 tactgacgcc tggcagtttc gccagcttcg cggaggtatc tgagatgcac agcataatgc
     1321 ggcgcagctg gctgcgctga ctcacgctta acggctcgta actttccata ttgcctggca
     1381 gcatcgtttt aacgcgcgca atcgcatcaa aggtatttgc cggatgacag tgaaactctg
     1441 ttacttgcgc gccatcagtc gatgccgcag gcaatggagg ttccatcgca atcaacttct
     1501 gcggcagttc aggatgctgt tgcagatagt gttcgaagtt ggagacggca tcgcgagtac
     1561 gggtaatttc atagccggac gcgttcatat tgacgacgaa gccagcaggg gcaatcccca
     1621 ccagtaccag catcaccagg ccgatccctt tctgcccgtc gttcgcgccg tgcgaaaacg
     1681 ccacgcccgc agcggaaaca atcagcgcaa tacgcgtcca gaatggcggt ttacgtttgc
     1741 cttttttctt tttgcgatct tccggaatgc ggtgaatacg gtcacgcttt ttcgtcccgc
     1801 tccagaaacg gcgcagcagg aaaatcagac cacccgcaat gaccaggccg acgatagggg
     1861 aaacaatcag cgaggagaaa attttggtca cttcacgcag gtttaacgca tccatcaccg
     1921 atgagccggt taacagcgcg ttggttaaac cgatgccgat aatcgcgcca atcaaggtgt
     1981 gcgaactgga ggccggtaaa ccgaagaacc acgtccccag gttccatata atcgccgcca
     2041 gcagcatgga aaagaccatc gccagaccgt gggttgaccc catattcagc aacaaatcgg
     2101 ttggtaacat atggacaatg gcataggcaa cgctaagtcc gcccaataac acgccaaaaa
     2161 agttaaaaaa tgccgccatc accacagcaa gttgtggttg catggcacga gtataaataa
     2221 cggttgccac cgcattcgcc gtgtcatgaa aaccattgat tgcttcgtag aacaacacaa
     2281 atgccagagc aagcaataac aaaagccctg tgtatatatc aaggccaaca aataaattta
     2341 gcatatatta gattacgcca ttttgaatt
//
LOCUS       DAZKHF020000404         2359 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2201S31241
            SAMN43267800-rid25106413.denovo.406, whole genome shotgun sequence.
ACCESSION   DAZKHF020000404 DAZKHF020000000
VERSION     DAZKHF020000404.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267800
            Sequence Read Archive: SRR30307263
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2359)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2359)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 2359)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350581.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350581.2
            Genome Coverage       :: 151x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:21:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,652
            CDSs (total)                      :: 5,557
            Genes (coding)                    :: 5,320
            CDSs (with protein)               :: 5,320
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 237
            CDSs (without protein)            :: 237
            Pseudo Genes (ambiguous residues) :: 0 of 237
            Pseudo Genes (frameshifted)       :: 102 of 237
            Pseudo Genes (incomplete)         :: 130 of 237
            Pseudo Genes (internal stop)      :: 52 of 237
            Pseudo Genes (multiple problems)  :: 41 of 237
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2359
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267800-rid25106413.denovo.406"
                     /serotype="O 26:H11"
                     /isolate="2201S31241"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-01"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            complement(<1..587)
                     /locus_tag="ACHOSC_RS23855"
                     /old_locus_tag="pgaptmp_004771"
     CDS             complement(<1..587)
                     /locus_tag="ACHOSC_RS23855"
                     /old_locus_tag="pgaptmp_004771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="major capsid protein"
                     /protein_id="HGU4550545.1"
                     /translation="MGLFTTRQLLGYTEQKVKFRALFLELFFRRTVNFHTEEVMLDKI
                     TGKTPVAAYVSPVVEGKVLRHRGGETRVLRPGYVKPKHEFNYQQAVERLPGEDPAQLN
                     DPAYRRLRIITDNLKQEEHAIVRVEEMQAVNAVLYGKYTMEGDQFEKIEVDFGRSTKN
                     NITQGSGKEWSKQDRDTFDPTHDLDLYCDQASGLV"
     gene            complement(645..992)
                     /locus_tag="ACHOSC_RS23860"
                     /old_locus_tag="pgaptmp_004772"
     CDS             complement(645..992)
                     /locus_tag="ACHOSC_RS23860"
                     /old_locus_tag="pgaptmp_004772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="head decoration protein"
                     /protein_id="HGU4550546.1"
                     /translation="MVTKTITEQRVEVRIFAGNDPAHTATGSSGISSATPALTPLMLD
                     EATGKLVVWDGQKAGSAVGILVLPLEGTETALTYYKSGTFATEAIRWPESVDEHKKAN
                     AFAGSALSHAALP"
     gene            complement(1029..>2359)
                     /locus_tag="ACHOSC_RS23865"
                     /old_locus_tag="pgaptmp_004773"
     CDS             complement(1029..>2359)
                     /locus_tag="ACHOSC_RS23865"
                     /old_locus_tag="pgaptmp_004773"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="S49 family peptidase"
                     /protein_id="HGU4550547.1"
                     /translation="EYMAGGKRPARVYRVVNGIAVLPVTGTLVHRLGGMRPFSGMTGY
                     DGIVACLQQAMADSQVRGVLLDIDSPGGQAAGAFDCADMIYRLRQQKPVWALCNDTAC
                     SAAMLLASACSRRLVTQTSRIGSIGVMMSHVSYAGHLAQAGVDITLIYSGAHKVDGNQ
                     FEALPAEVRQDMQQRIDAARRMFAEKVAMYTGLSVDAVTGTEAAVFEGQSGIEAGLAD
                     ELINASDAISVMATALNSNVRGGTMPQLTATEAAAQENQRVMGILTCQEAKGREQLAT
                     MLAGQQGMSVEQARAILAAAAPQQPVASAQSEADRIMACEEAKGREQLAATLAAMPEM
                     TVEKARPILAASPQADAGPSLRDQIMALDEAKGAEAQAEQLAACPGMTVESARAVLAA
                     GSGKAEPVSASTTAMFERIMANHSPAAVQGGVPQTSADGDADVKMLMAMP"
BASE COUNT          466 a          754 c          602 g          537 t
ORIGIN      
        1 ttcacaagac cgctggcctg gtcgcagtag aggtcaagat catgtgtagg atcgaacgtg
       61 tcacgatcct gttttgacca ctccttacca ctaccctgag tgatgttatt cttcgtcgac
      121 ctgccaaaat cgacctcaat tttctcgaac tggtctcctt ccatcgtgta tttgccatac
      181 agcacggcat tcaccgcctg catttcttcc acccggacaa tggcgtgctc ttcctgtttg
      241 aggttatcag tgatgatacg cagacgacgg taggccgggt cgttcagctg agccggatct
      301 tcaccgggaa gacgctcaac cgcctgctgg taattaaatt cgtgtttcgg cttgacgtag
      361 cccggacgta acacgcgggt ttcaccacca cgatgacgca gcacttttcc ttcaacaacc
      421 ggggaaacat aggccgccac cggcgttttt ccggtaattt tgtccagcat cacctcttcg
      481 gtatggaaat tcaccgtacg gcggaaaaac agctccagaa acagcgcacg aaatttcact
      541 ttttgttcgg tataaccgag taactggcgg gtcgtaaaca atcccataaa tcagttcctt
      601 tcattcagaa atcagtcagg ccaccgcggt ggcctgataa cgtgttacgg cagcgccgcg
      661 tgactcaggg cactgccggc aaaggcgttg gcctttttgt gttcatccac actttcaggc
      721 cagcggattg cctccgtcgc aaaggtcccc gacttgtaat aggtcagcgc cgtctctgtg
      781 ccttcaagcg gcagtaccag tatgccaacc gcactaccgg ctttctgtcc gtcccagacc
      841 accagtttcc cggtggcctc atccagcatc aggggcgtca gtgccggtgt tgccgaggaa
      901 atcccgctgc tgcctgtggc ggtatgagcc ggatcattac cggcaaaaat acgtacttcc
      961 acacgctgtt cagtgatagt tttcgtcacc atattgtaaa aacctcctgt tgatggtcag
     1021 cactggcttc atggcatggc catgagcatt ttcacgtccg catcaccgtc tgctgacgtc
     1081 tgtggcacgc caccctgtac cgctgccggt gaatggttcg ccatgatgcg ttcaaacatg
     1141 gcggttgtgg atgcagagac cggttctgcc ttacctgatc ccgcagccag cacagcccgg
     1201 gcgctctcca cagtcattcc cgggcaggca gccagctgtt cagcctgcgc ctcagcccct
     1261 tttgcctcat ccagtgccat gatctgatca cggagtgagg gtccggcatc cgcctgcggt
     1321 gaagcagcca ggatcgggcg ggctttttcc accgtcatct ccggcatcgc cgccagcgtt
     1381 gccgccagtt gttcacgacc tttcgcttct tcacacgcca taatgcgatc ggcttcactc
     1441 tgcgcggatg ccaccggctg ctgtggtgcc gccgcggcca gaatcgcccg ggcctgttca
     1501 acgctcatgc cctgttgtcc tgccagcatc gtggcaagct gttcacgtcc tttcgcttcc
     1561 tggcatgtca ggatccccat cactcgctgg ttctcctgcg cggcggcttc cgttgcagtt
     1621 aattgcggca tagtgcctcc tctgacatta ctgttcagcg ccgtggccat cacactgatg
     1681 gcatccgacg cattgattaa ttcatccgcc agcccggcct caatgccgga ctgaccttca
     1741 aaaacggcgg cctctgttcc cgtgacagca tcaacagaca gaccggtata catcgctact
     1801 ttttcagcaa acatccggcg cgccgcatca atgcgctgtt gcatgtcctg gcgaacctct
     1861 gccggcaacg cttcaaactg attgccatcc accttgtgcg cccctgagta aatcagcgtg
     1921 atatcaacac cggcctgcgc cagatgaccg gcatagctga catggctcat catcacgcca
     1981 atggagccga tacgggatgt ctgggtaacc agccgtcggg agcaggccga cgccagcagc
     2041 atggctgcag agcaggccgt gtcattgcac agtgcccaga ccggcttctg ctgacggagg
     2101 cggtaaatca tgtcagcgca gtcaaacgcg ccggcggcct gcccgcccgg actgtcaatg
     2161 tccagcagta cgccccgcac ctggctatcc gccattgcct gctgaagaca ggcgacaatg
     2221 ccgtcatagc ctgtcattcc ggaaaatggc cgcatacccc ccagccggtg caccagcgtg
     2281 ccggtcaccg gcagtacagc aataccgttc accacccggt aaacacgggc cggtcgttta
     2341 cctccggcca tgtactcgt
//
LOCUS       DAZKHF020000405         2353 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2201S31241
            SAMN43267800-rid25106413.denovo.407, whole genome shotgun sequence.
ACCESSION   DAZKHF020000405 DAZKHF020000000
VERSION     DAZKHF020000405.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267800
            Sequence Read Archive: SRR30307263
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2353)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2353)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 2353)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350581.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350581.2
            Genome Coverage       :: 151x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:21:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,652
            CDSs (total)                      :: 5,557
            Genes (coding)                    :: 5,320
            CDSs (with protein)               :: 5,320
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 237
            CDSs (without protein)            :: 237
            Pseudo Genes (ambiguous residues) :: 0 of 237
            Pseudo Genes (frameshifted)       :: 102 of 237
            Pseudo Genes (incomplete)         :: 130 of 237
            Pseudo Genes (internal stop)      :: 52 of 237
            Pseudo Genes (multiple problems)  :: 41 of 237
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2353
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267800-rid25106413.denovo.407"
                     /serotype="O 26:H11"
                     /isolate="2201S31241"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-01"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            <1..245
                     /locus_tag="ACHOSC_RS23870"
                     /old_locus_tag="pgaptmp_004774"
     CDS             <1..245
                     /locus_tag="ACHOSC_RS23870"
                     /old_locus_tag="pgaptmp_004774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF3302 domain-containing protein"
                     /protein_id="HGU4550548.1"
                     /translation="IPYLIAKKRNHPHADAIHVAGWVSLFTLHVIWPFLWIWATLYRP
                     ERGWGMQNHDSSVVQLQQRIAGLEKQLADIKSSSAE"
     gene            251..1387
                     /locus_tag="ACHOSC_RS23875"
                     /old_locus_tag="pgaptmp_004775"
     CDS             251..1387
                     /locus_tag="ACHOSC_RS23875"
                     /old_locus_tag="pgaptmp_004775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family secretion protein"
                     /protein_id="HGU4550549.1"
                     /translation="MDLLIILTYVAFAWAMFKIFKIPVNKWTIPTAALGGIFIVSGLI
                     LLMNYNHPYTFKAQKAVISIPVVPQVTGVVIEVTDKKNTLIKKGEVLFRLDPTRYQAR
                     VDRLMADIVTAEHKQRALGAELDEMAANTQQAKATRDKFAKEYKRYARGSQAKVNPFS
                     ERDIDVARQNYLAQEASVKSSAAEQKQIQSQLDSLVLGEHSQIASLKAQLAEAKYNLE
                     QTIVRAPSDGYVTQVLIRPGTYAASLPLRPVMVFIPDQKRQIVAQFRQNSLLRLAPGD
                     DAEVVFNALPGKVFSGKLAAISPAVPGGAYQSTGTLQTLNTAPGSDGVIATIELDEHT
                     DLSALPDGIYAQVAVYSDHFSHVSVMRKVLLRMTSWVHYLYLDH"
     gene            complement(1384..>2353)
                     /locus_tag="ACHOSC_RS23880"
                     /old_locus_tag="pgaptmp_004776"
     CDS             complement(1384..>2353)
                     /locus_tag="ACHOSC_RS23880"
                     /old_locus_tag="pgaptmp_004776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HGU4550550.1"
                     /translation="KLQLKYRELKIISVIAASENISHAATVLGIAQANVSKYLADFES
                     KVGLKVFDRTTRQLMLTPFGTALLPYINDMLDRNEQLNNFIADYKHEKRGRVTIYAPT
                     GIITYLSKHVIDKIKDIGDITLSLKTCNLERNAFYEGVEFPDDCDVLISYAPPKDESL
                     VASFITQYAVTAYASQRYLEKHPISRPDELEHHSCILIDSMMIDDANIWRFNVAGSKE
                     VRDYRVKGNYVCDNTQSALELARNHLGIVFAPDKSVQSDLQDGTLVPCFQQPYEWWLD
                     LVAIFRKREYQPWRVQYVLDEMLREIRHQLAQSQQLRPEQAAESED"
BASE COUNT          578 a          556 c          607 g          612 t
ORIGIN      
        1 atattcccta cctgattgct aaaaaacgta atcatcctca tgccgacgcc attcatgttg
       61 ctggttgggt gagtcttttt acgctgcatg ttatctggcc atttttgtgg atttgggcca
      121 cgctttaccg cccggagcgg ggatggggaa tgcaaaacca tgactcctcc gttgtgcaac
      181 tacaacagcg tattgccggg ctggagaaac agctcgccga catcaaatcc tcttctgccg
      241 agtaatactt atggatttac tgattatttt gacctatgtg gcttttgcat gggcaatgtt
      301 taagatcttc aaaattcctg taaataaatg gaccattccc acagcggccc tgggtggcat
      361 atttattgtc agtggtttaa ttctgttaat gaactataac catccgtata ccttcaaagc
      421 gcaaaaagcg gttatttcta ttcctgttgt cccacaggtg acaggcgtgg tgatcgaagt
      481 gacggataag aaaaatacgc tgattaaaaa aggtgaggtg ctatttcgac tggacccgac
      541 gcgttatcag gcgcgggtgg atcggctgat ggcggatatc gttaccgcag aacataaaca
      601 gcgggcgttg ggcgcagagt tagatgagat ggcggcgaat actcagcagg caaaggccac
      661 gcgggataaa ttcgctaaag agtataagcg ttacgcacgt ggcagccagg cgaaagtaaa
      721 cccgttttca gaacgcgata tcgatgtggc gcggcaaaat tatctggcgc aggaagcctc
      781 cgtgaagtca tcggcggcgg aacaaaaaca gatccagagc cagctggata gcctggtgtt
      841 gggtgaacat tctcaaatcg ccagcctgaa agcacagctc gcggaagcaa aatataacct
      901 tgagcagacg atagtgcgtg cgccgagcga tggttatgtg acccaggtac tgattcgtcc
      961 gggtacctat gccgcgtcgc tgccgctacg tccggtgatg gtgtttatac ccgatcagaa
     1021 acgacaaatc gtggcgcagt tccgtcagaa ctccttgctg cgactggctc ctggcgacga
     1081 tgcggaagtg gtgtttaatg ctctgccagg taaggtattc agcggtaagc tggcagccat
     1141 tagtccagcc gttcccggcg gagcttatca gtcgaccggc accttacaga cgttaaacac
     1201 agcgccgggt tcagatggcg ttatcgcgac cattgaactg gatgagcaca ctgatttgag
     1261 cgctttacca gacggtattt acgcccaggt ggcggtttac tctgatcatt tcagccatgt
     1321 ctcggtgatg cgcaaagtgc tattacgcat gaccagctgg gtgcattacc tttatctcga
     1381 tcattaatct tcgctttccg ccgcttgttc aggccgcagt tgctgcgact gagcaagctg
     1441 gtggcgaatt tcacgcagca tttcatccag cacatactgg acccgccagg gctggtattc
     1501 ccgtttacgg aagatagcca ccagatccag ccaccattca tagggttgct gaaagcaggg
     1561 caccagcgtg ccgtcttgca ggtcgctctg cacacttttg tctggcgcaa acacaatccc
     1621 caggtgattt cgtgccagct ccagcgcaga ttgcgtgttg tcgcaaacgt aatttccctt
     1681 cacgcgataa tcccgcacct ctttactccc ggcgacgttg aagcgccaga tattcgcgtc
     1741 gtcgatcatc atcgagtcga tcaaaataca ggagtgatgt tcgagttcgt caggacggct
     1801 aatgggatgt ttctcaagat agcgctggct ggcataagcg gttacggcat attgggtaat
     1861 aaaactggca accagcgatt catcttttgg tggagcgtag ctgattaaaa catcacaatc
     1921 atcaggaaat tcgacgcctt cataaaaggc attacgctca agattgcagg tttttagcga
     1981 taaggtgata tcaccgatat ctttaatttt atcgattaca tgtttggata aataggtaat
     2041 tatgccggtt ggggcgtaga tggtgacccg accacgtttc tcatgcttat aatctgcaat
     2101 aaaattatta agttgctcgt ttctgtccag catgtcgttg atgtacggta gcagcgcggt
     2161 gccaaaaggt gtgagcatca gctgccgggt ggttcggtca aaaactttta aacccacttt
     2221 tgattcaaaa tcagcaagat atttgctgac gttggcctgt gcgatgccaa gtacagtcgc
     2281 ggcatggctg atattttcac tggcagcgat taccgagata atttttaact cccggtactt
     2341 aagttgtaat ttt
//
LOCUS       DAZKHF020000406         2353 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2201S31241
            SAMN43267800-rid25106413.denovo.408, whole genome shotgun sequence.
ACCESSION   DAZKHF020000406 DAZKHF020000000
VERSION     DAZKHF020000406.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267800
            Sequence Read Archive: SRR30307263
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2353)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2353)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 2353)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350581.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350581.2
            Genome Coverage       :: 151x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:21:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,652
            CDSs (total)                      :: 5,557
            Genes (coding)                    :: 5,320
            CDSs (with protein)               :: 5,320
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 237
            CDSs (without protein)            :: 237
            Pseudo Genes (ambiguous residues) :: 0 of 237
            Pseudo Genes (frameshifted)       :: 102 of 237
            Pseudo Genes (incomplete)         :: 130 of 237
            Pseudo Genes (internal stop)      :: 52 of 237
            Pseudo Genes (multiple problems)  :: 41 of 237
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2353
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267800-rid25106413.denovo.408"
                     /serotype="O 26:H11"
                     /isolate="2201S31241"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-01"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            <1..784
                     /gene="nirC"
                     /locus_tag="ACHOSC_RS23885"
                     /old_locus_tag="pgaptmp_004777"
     CDS             <1..784
                     /gene="nirC"
                     /locus_tag="ACHOSC_RS23885"
                     /old_locus_tag="pgaptmp_004777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026212.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="nitrite transporter NirC"
                     /protein_id="HGU4550551.1"
                     /translation="CAANAARIARLSANNPLGFWVSSAMAGAYVGLGIILIFTLSNLL
                     DPSVRPLVMGATFGIALTLVIIAGSELFTGHTMFLTFGVKAGSISHGQMWAILPQTWL
                     GNLVGSVFVAMLYSWGGGSLLPVDTSIVHSVALAKTTAPAMVLFFKGALCNWLVCLAI
                     WMALRTEGAAKFIAIWWCLLAFIASGYEHSIANMTLFALSWFGNHSEAYTLAGIGHNL
                     LWVTLGNTLSGAVFMGLGYWYATPKANRPVADKFNQTETAAG"
     gene            803..2176
                     /gene="cysG"
                     /locus_tag="ACHOSC_RS23890"
                     /old_locus_tag="pgaptmp_004778"
     CDS             803..2176
                     /gene="cysG"
                     /locus_tag="ACHOSC_RS23890"
                     /old_locus_tag="pgaptmp_004778"
                     /EC_number="1.3.1.76"
                     /EC_number="2.1.1.107"
                     /EC_number="4.99.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004851 - uroporphyrin-III
                     C-methyltransferase activity [Evidence IEA];
                     GO_function: GO:0043115 - precorrin-2 dehydrogenase
                     activity [Evidence IEA];
                     GO_function: GO:0051266 - sirohydrochlorin ferrochelatase
                     activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0019354 - siroheme biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="siroheme synthase CysG"
                     /protein_id="HGU4550552.1"
                     /translation="MDHLPIFCQLRDRDCLIVGGGDVAERKARLLLDAGARLTVNALA
                     FIPQFTAWADAGMLTLVEGPFDESLLDTCWLAIAATDDDTLNQRVSEAAEARRIFCNV
                     VDAPKAASFIMPSIIDRSPLMVAVSSGGTSPVLARLLREKLESLLPLHLGQVAKYAGQ
                     LRGRVKQQFATMSERRRFWEKLFVNDRLAQSLANNDQKAITETTEQLINEPLDHRGEV
                     VLVGAGPGDAGLLTLKGLQQIQQADVVVYDRLVSDDIMNLVRRDADRVFVGKRAGYHC
                     VPQEEINQILLREAQKGKRVVRLKGGDPFIFGRGGEELETLCNAGIPFSVVPGITAAS
                     GCSAYSGIPLTHRDYAQSVRLITGHLKTGGELDWENLAAEKQTLVFYMGLNQAATIQQ
                     KLIEHGMPGEMPVAIVENGTAVTQRVIDGTLTQLGELAQQMNSPSLIIIGRVVGLRDK
                     LNWFSNH"
BASE COUNT          515 a          595 c          658 g          585 t
ORIGIN      
        1 gtgtgcggct aatgctgcac gcattgcacg cctgtcggca aataacccgc tcggcttttg
       61 ggtcagctcc gccatggcgg gcgcatatgt gggtcttggg atcatcctga ttttcacgct
      121 cagtaatttg ctcgacccgt ccgtacgtcc gctggtgatg ggcgcgacct ttggtatcgc
      181 cttaacgctg gtgattatcg ccggttctga actgttcacc ggacacacca tgttcctcac
      241 ctttggggta aaagcgggca gcatcagcca cgggcaaatg tgggcaatcc tgccgcaaac
      301 ctggctgggt aacctggtcg gttccgtctt cgttgccatg ctctatagct ggggcggcgg
      361 tagcctgctg ccggtagata ccagcatcgt tcactccgtc gcgctggcta aaaccactgc
      421 accggcaatg gtactgttct tcaaaggtgc attgtgtaac tggctggttt gcctggcaat
      481 ctggatggcg ctgcgcactg aaggggcggc gaaatttatc gctatctggt ggtgtctgct
      541 ggcatttatc gcgtccggct acgagcactc tatcgctaac atgacgctgt tcgcgctctc
      601 ctggttcggc aaccacagcg aagcctacac gctggcgggt attggtcata acctgctgtg
      661 ggtgacgctg ggtaatactt tatcaggtgc cgtattcatg ggattgggtt attggtatgc
      721 tacgccgaaa gcgaatcgtc cggttgcgga caaatttaat caaactgaaa cggctgccgg
      781 ttaattacta aggggttttt acgtggatca tttgcctata ttttgccaat tacgcgatcg
      841 cgactgtctg attgtcggcg gtggtgatgt cgcggaacgc aaagcaaggt tgctgttaga
      901 cgcaggcgct cgcttaacgg tgaatgcatt agcgtttatt ccacagttca ccgcatgggc
      961 agatgcaggc atgttaaccc tcgtcgaagg gccatttgat gaaagccttc tcgacacctg
     1021 ctggctggcg attgcagcga cggatgatga cacgcttaac cagcgcgtca gcgaagccgc
     1081 tgaagctcgt cgcatcttct gtaacgtagt cgatgcgccg aaagccgcca gctttattat
     1141 gccgtcgatt attgaccgct caccgctaat ggtagcggtc tcctctggcg gcacctctcc
     1201 ggttctggcg cgcctgttgc gcgaaaaact cgaatcactg ctgccgttgc atctgggcca
     1261 ggtggcgaaa tatgccgggc aattacgcgg gcgagtgaaa caacagttcg ccacgatgag
     1321 tgagcgtcgc cgtttctggg agaaattgtt cgttaacgac cgcctggcgc agtcgctggc
     1381 aaacaacgat cagaaagcca ttactgaaac gaccgaacag ttaatcaacg aaccgctcga
     1441 ccatcgcggt gaagtggtgc tggttggtgc aggtccgggc gatgccgggc tgctgacgct
     1501 gaaaggactg caacaaattc agcaggcaga tgtggtggtc tacgaccgtc tggtttctga
     1561 cgatattatg aatctggtac gccgcgatgc tgatcgcgtt ttcgtcggca aacgcgcggg
     1621 ataccactgt gtaccgcagg aagagattaa ccagatcctg ctgcgggaag cgcaaaaagg
     1681 caaacgcgtg gtgcggctga aaggtggcga tccgtttatt tttggccgtg gtggcgaaga
     1741 gctggaaaca ctgtgcaacg cgggtattcc gttctcggtg gttccgggta ttaccgcagc
     1801 ttctggttgc tctgcctatt cgggtattcc gctcacgcat cgcgattatg cccagagcgt
     1861 gcgcttaatt accggacact taaaaaccgg tggcgagctg gactgggaaa acctggcggc
     1921 agaaaaacag acgctggtgt tctatatggg gctgaatcag gctgcgacta ttcagcaaaa
     1981 gctgattgaa cacggtatgc ctggcgaaat gccggtggca attgtcgaaa acggaacagc
     2041 agtcacgcag cgcgtgattg acggtacgct cacgcaactc ggtgaacttg ctcagcaaat
     2101 gaacagtcca tcgctaatta ttattggtcg ggttgttggc ctgcgcgata aactgaactg
     2161 gttctccaac cattaattta acccgggcca gagaattctg gccttcttaa tgactctttt
     2221 tatatattca gcaaataaaa catagcccct taataaatat tatctgctaa tgggttatat
     2281 tcaccgttac agcgacttac aaaattaagt cattcgctca ctgacgccaa ttaaatattc
     2341 ctgtatcgtt ctt
//
LOCUS       DAZKHF020000407         2345 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2201S31241
            SAMN43267800-rid25106413.denovo.409, whole genome shotgun sequence.
ACCESSION   DAZKHF020000407 DAZKHF020000000
VERSION     DAZKHF020000407.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267800
            Sequence Read Archive: SRR30307263
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2345)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2345)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 2345)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350581.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350581.2
            Genome Coverage       :: 151x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:21:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,652
            CDSs (total)                      :: 5,557
            Genes (coding)                    :: 5,320
            CDSs (with protein)               :: 5,320
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 237
            CDSs (without protein)            :: 237
            Pseudo Genes (ambiguous residues) :: 0 of 237
            Pseudo Genes (frameshifted)       :: 102 of 237
            Pseudo Genes (incomplete)         :: 130 of 237
            Pseudo Genes (internal stop)      :: 52 of 237
            Pseudo Genes (multiple problems)  :: 41 of 237
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2345
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267800-rid25106413.denovo.409"
                     /serotype="O 26:H11"
                     /isolate="2201S31241"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-01"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            <1..>2345
                     /gene="agn43"
                     /locus_tag="ACHOSC_RS23895"
                     /old_locus_tag="pgaptmp_004779"
                     /gene_synonym="flu"
     CDS             <1..>2345
                     /gene="agn43"
                     /locus_tag="ACHOSC_RS23895"
                     /old_locus_tag="pgaptmp_004779"
                     /gene_synonym="flu"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter adhesin Ag43"
                     /protein_id="HGU4550553.1"
                     /translation="TGAFVVASELARARGKRGGVAVALSLAAVTSLPVLAADIVVHPG
                     ETVNGGTLANHDNQIVFGTTNGMTISTGLEYGPDNEANTGGQWVQDGGTANKTTVTSG
                     GLQRVNPGGSVSDTVISAGGGQSLQGRAVNTTLNGGEQWMHEGAIATGTVINDKGWQV
                     VKPGTVATDTVVNTGAEGGPDAENGDTGQFVRGDAVRTTINKNGRQIVRTEGTANTTV
                     VYAGGDQTVHGHALDTTLNGGYQYVHNGGTASDTVVNSDGWQIVKNGGVAGNTTVNQK
                     GRLQVDAGGTATNVTLKQGGALVTSTAATVTGINRLGAFSVVEGKADNVVLENGGRLD
                     VLTGHTATNTRVDDGGTLDVRNGGTATTVSMGNGGVLLADSGAAVSGTRSDGKAFSIG
                     GGQADALMLEKGSSFTLNAGDTATDTTVNGGLFTARGGTLAGTTTLNNGAILTLSGKT
                     VNNDTLTIREGDALLQGGSLTGNGSVEKSGSGTLTVSNTTLTQKAVNLNEGTLTLNDS
                     TVTTDVIAQRGTALKLTGSTVLNGAIDPTNVTLASGATWNIPDNATVQSVVDDLSHAG
                     QIHFTSTRTGKFVPATLKVKNLNGQNGTISLRVRPDMAQNNADRLVIDGGRATGKTIL
                     NLVNAGNSASGLATSGKGIQVVEAINGATTEEGAFVQGNRLQAGAFNYSLNRDSDESW
                     YLRSENAYRAEVPLYASMLTQAMDYDRIVAGSRSHQTGVNGENNSVRLSIQGGHLGHD
                     NNGGIARGATPESSGSYGFVRLEGDLMRTEVAGMSVTAGVYG"
BASE COUNT          567 a          628 c          736 g          414 t
ORIGIN      
        1 acgggcgctt tcgtggttgc ctccgaactg gcccgcgcac ggggtaaacg tggcggtgtg
       61 gcggttgcac tgtctcttgc cgcagtcacg tcactcccgg tgctggctgc tgacatcgtt
      121 gtgcacccgg gagaaaccgt gaacggcgga acactggcaa atcatgacaa ccagattgtc
      181 ttcggtacga ccaacggaat gaccatcagt accgggctgg agtatgggcc ggataacgag
      241 gccaataccg gcgggcaatg ggtacaggat ggcggaacag ccaacaaaac gactgtcacc
      301 agtggtggtc ttcagagagt gaaccccggt ggaagtgtct cagacacggt tatcagtgcc
      361 ggaggcggac agagccttca gggacgggca gtgaacacca cgctgaatgg tggcgaacag
      421 tggatgcatg agggggcgat agccacagga accgtcatta atgataaggg ctggcaggtc
      481 gtcaagcccg gtacagtggc aacggatacc gttgttaata ccggggcgga agggggaccg
      541 gatgcagaaa acggtgatac cgggcagttt gttcgcgggg atgccgtacg cacaaccatc
      601 aataaaaacg gtcgccagat tgtgagaact gaaggaacgg caaataccac tgtggtttat
      661 gccggcggcg accagactgt acatggtcac gcactggata ccacgctgaa tgggggatac
      721 cagtatgtgc acaacggcgg tacagcgtct gacactgttg tgaacagtga cggctggcag
      781 attgtcaaaa acgggggtgt ggccgggaat accaccgtta atcagaaggg cagactgcag
      841 gtggacgccg gtggtacagc cacgaatgtc accctgaagc agggcggcgc actggttacc
      901 agtacggctg caaccgttac cggcataaac cgcctgggag cattctctgt tgtggagggt
      961 aaagctgata atgtcgtact ggaaaatggc ggacgcctgg atgtgctgac cggacacaca
     1021 gccactaata cccgcgtgga tgatggcgga acgctggatg tccgcaacgg tggcaccgcc
     1081 accaccgtat ccatgggaaa tggcggtgta ctgctggccg attccggtgc cgctgtcagt
     1141 ggtacccgga gcgacggaaa ggcattcagt atcggaggcg gtcaggcgga tgccctgatg
     1201 ctggaaaaag gcagttcatt cacgctgaac gccggtgata cggccacgga taccacggta
     1261 aatggcggac tgttcaccgc caggggcggc acactggcgg gcaccaccac gctgaataac
     1321 ggcgccatac ttaccctttc cgggaagacg gtgaacaacg ataccctgac catccgtgaa
     1381 ggcgatgcac tcctgcaggg aggctctctc accggtaacg gcagcgtgga aaaatcagga
     1441 agtggcacac tcactgtcag caacaccaca ctcacccaga aagccgtcaa cctgaatgaa
     1501 ggcacgctga cgctgaacga cagtaccgtc accacggatg tcattgctca gcgcggtaca
     1561 gccctgaagc tgaccggcag cactgtgctg aacggtgcca ttgaccccac gaatgtcact
     1621 ctcgcctccg gtgccacctg gaatatcccc gataacgcca cggtgcagtc ggtggtggat
     1681 gacctcagcc atgccggaca gattcatttc acctccaccc gcacagggaa gttcgtaccg
     1741 gcaaccctga aagtgaaaaa cctgaacgga cagaatggca ccatcagcct gcgtgtacgc
     1801 ccggatatgg cacagaacaa tgctgacaga ctggtcattg acggcggcag ggcaaccgga
     1861 aaaaccatcc tgaacctggt gaacgccggc aacagtgcgt cggggctggc gaccagcggt
     1921 aagggtattc aggtggttga agccattaac ggtgccacca cggaggaagg ggcctttgtc
     1981 caggggaaca ggctgcaggc cggtgccttt aactactccc tcaaccggga cagtgatgag
     2041 agctggtatc tgcgcagtga aaatgcttat cgtgcagaag tccccctgta tgcctccatg
     2101 ctgacacagg caatggacta tgaccggatt gtggcaggct cccgcagcca tcagaccggt
     2161 gtaaatggtg aaaacaacag cgtccgtctc agcattcagg gcggtcatct cggtcacgat
     2221 aacaatggcg gtattgcccg tggggccacg ccggaaagca gcggcagcta tggattcgtc
     2281 cgtctggagg gtgacctgat gagaacagag gttgccggta tgtctgtgac cgcgggggta
     2341 tatgg
//
LOCUS       DAZKHF020000408         2310 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2201S31241
            SAMN43267800-rid25106413.denovo.410, whole genome shotgun sequence.
ACCESSION   DAZKHF020000408 DAZKHF020000000
VERSION     DAZKHF020000408.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267800
            Sequence Read Archive: SRR30307263
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2310)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2310)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 2310)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350581.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350581.2
            Genome Coverage       :: 151x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:21:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,652
            CDSs (total)                      :: 5,557
            Genes (coding)                    :: 5,320
            CDSs (with protein)               :: 5,320
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 237
            CDSs (without protein)            :: 237
            Pseudo Genes (ambiguous residues) :: 0 of 237
            Pseudo Genes (frameshifted)       :: 102 of 237
            Pseudo Genes (incomplete)         :: 130 of 237
            Pseudo Genes (internal stop)      :: 52 of 237
            Pseudo Genes (multiple problems)  :: 41 of 237
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2310
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267800-rid25106413.denovo.410"
                     /serotype="O 26:H11"
                     /isolate="2201S31241"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-01"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            37..411
                     /locus_tag="ACHOSC_RS23900"
                     /old_locus_tag="pgaptmp_004780"
     CDS             37..411
                     /locus_tag="ACHOSC_RS23900"
                     /old_locus_tag="pgaptmp_004780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RusA family crossover junction
                     endodeoxyribonuclease"
                     /protein_id="HGU4550554.1"
                     /translation="MRIEFVLLYPPTVNTYWRRRGSTYFVSKAGERYRRAVALIVRQQ
                     RLKLSLSGRLAIKIIAEPPDKRRRDLDNILKAPLDALTHAGVLMDDEQFDEINIVRGQ
                     PVSGGRLGVKIYPIMLEGLVKK"
     gene            408..1229
                     /locus_tag="ACHOSC_RS23905"
                     /old_locus_tag="pgaptmp_004781"
     CDS             408..1229
                     /locus_tag="ACHOSC_RS23905"
                     /old_locus_tag="pgaptmp_004781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="antitermination protein"
                     /protein_id="HGU4550555.1"
                     /translation="MKLEDLPKYYSPKSPGLTDASASTSKDTLSITDVMAAQGMTQNW
                     AEMGFSAFLGKMGISMNDRERATELLTEYALSRCDRVAALRKLPAEIKPAVMRIMASY
                     AFEDYARSAASKKQCPCCHGKKFIESEVFTNKIQYPDGKPPVWAKCTKGVYPSYWEEW
                     KKVREVVKVACPECGGKGEVSTACKDCRGRGVAIHREESVKRGMPVIRDCQRCGGRGY
                     ERLPSTEAFNAICNVTDAISLDTWKKTVKRFYDTLVVQFDIEEAWAEQQLKKVTR"
     gene            1467..1829
                     /locus_tag="ACHOSC_RS23910"
                     /old_locus_tag="pgaptmp_004782"
                     /pseudo
     CDS             1467..1829
                     /locus_tag="ACHOSC_RS23910"
                     /old_locus_tag="pgaptmp_004782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001448682.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tellurite resistance TerB family protein"
     gene            1826..1993
                     /locus_tag="ACHOSC_RS23915"
                     /old_locus_tag="pgaptmp_004783"
     CDS             1826..1993
                     /locus_tag="ACHOSC_RS23915"
                     /old_locus_tag="pgaptmp_004783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001357489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3927 family protein"
                     /protein_id="HGU4550556.1"
                     /translation="MTVKLRLAVAALLLFLVVMVDFTSRIMSVLADGVLVCGIVVLLW
                     PVIKRNSLHNA"
BASE COUNT          581 a          458 c          679 g          592 t
ORIGIN      
        1 tttgcaaccg gcgtgctcgg gtaaaagagg ttactgatgc gtatagagtt tgttttgctt
       61 tacccgccga cggtgaacac ctactggcga cgtcgtggca gcacatattt tgtatcaaaa
      121 gccggtgagc gttatcgccg ggctgtggcg cttattgttc gccagcagcg gctgaaatta
      181 agcctgtccg gaaggctggc gataaagatt attgccgagc caccggataa gcgccgccgt
      241 gacctggaca atattctgaa agcgccgctg gatgcgctga cgcatgcggg agtgttaatg
      301 gacgatgagc agtttgatga aatcaatatc gttcgtggtc agccagtatc tggtggacgt
      361 ctgggggtga agatttaccc cataatgctt gaagggctgg tcaaaaaatg aaactggaag
      421 atttaccgaa atactactcc ccaaaatccc ccggcctgac tgatgcatcg gcctcaacgt
      481 cgaaagatac gctgagtatc actgatgtga tggccgcgca gggcatgaca cagaattggg
      541 ctgagatggg gttttctgcg ttccttggga aaatgggcat tagtatgaat gacagagagc
      601 gggcaacaga attgctgaca gaatatgcac tcagtcggtg tgatcgcgtg gcggcgttaa
      661 gaaaactccc ggcagaaata aaaccggcag tgatgcgtat tatggcttcg tatgcgtttg
      721 aagattatgc ccgtagcgcg gcgagcaaaa aacagtgccc ctgttgtcac ggaaaaaaat
      781 ttattgaaag cgaggttttt acaaacaaga tccagtatcc ggatggtaag ccgccagtgt
      841 gggcaaagtg cacaaaaggc gtgtatccgt cttactggga ggaatggaaa aaagtcaggg
      901 aggtggtaaa agttgcctgt ccggagtgtg gagggaaggg ggaggtttcc accgcctgta
      961 aagattgtcg tgggcgcggt gttgccattc atcgtgaaga gtcggtaaaa cgtggtatgc
     1021 ctgttatcag agactgccag cgttgtggtg gtcgtggcta tgaaagatta ccttcaacgg
     1081 aggcatttaa tgccatatgt aatgtaaccg atgccatatc tcttgataca tggaaaaaaa
     1141 cagttaaacg tttttacgat acgctggtgg tgcagtttga tattgaagaa gcatgggcag
     1201 aacaacaact gaaaaaggtg accagatagc tttgttgatt tttcccgaat ctgtggtaaa
     1261 tttgccctaa cgatgggcgt tttatgcctg acgttagaag attttttaca cccgtcgcca
     1321 ggcgggtttt tttatgactg aaatcacgcc agtacagtaa acgcgctggt ggttgtgaat
     1381 accggtcttt cagcttgctg gctttttcga caagagttat tggtgtgtca cgttaaccgg
     1441 aaaaaggaaa gtttgagaaa cgcgatctgg cacaggcggt tattaatgct gcctacctgg
     1501 tggcctgtgc agatggtgaa tgtgaggctt cctagaaagc gaagatcgaa caggtactgc
     1561 gtaatcagcc tgcgctgtcc gcgtttacgt cagaaattaa tgcgattagc gcaaccatta
     1621 tcggtcagct ggatacgaac tttaaaattg gtcgtcgtgc ggcgttacgt gagatcgagg
     1681 atgtgaaaca cgatacgcgt gaagcggaag atgtgctgga tgtggcggtg gccattgcgg
     1741 aggcagacgg cgaaattgag ccggaagagc gcaaggtgct ggaagagatt gccggtgttc
     1801 tgggtcttcg tctggagaat caccagtgac ggtaaaactg cgcctggctg tggctgcact
     1861 cctgctgttt ctggtggtga tggtggattt caccagcaga atcatgtcgg tgctggcgga
     1921 tggggtgctg gtctgcggca ttgtggtatt gctgtggccg gtgataaaaa gaaacagcct
     1981 gcataatgct tgattttttt gtttactgtt tattaaaaat actactgcat ggtgaatccc
     2041 cctgtgcgga ggggcaatca gcaaccaggt atatgtgata atcgcggatt caggtgctga
     2101 tactgaattc accgggaggc acccggcacc atgcaagaaa aagaatgtgc atgcaaacat
     2161 gcccctctcc ggaggggctt ttttatgggt aaaaaatgcc cgaatgggtt cgggcaatag
     2221 catgagatac tgatattgtt gtgttgttat cgtgtggatt ttaaccaggg tttatcaggc
     2281 tgcgcaactg cgtggccttt tttcatttct
//
LOCUS       DAZKHF020000409         2308 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2201S31241
            SAMN43267800-rid25106413.denovo.411, whole genome shotgun sequence.
ACCESSION   DAZKHF020000409 DAZKHF020000000
VERSION     DAZKHF020000409.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267800
            Sequence Read Archive: SRR30307263
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2308)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2308)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 2308)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350581.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350581.2
            Genome Coverage       :: 151x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:21:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,652
            CDSs (total)                      :: 5,557
            Genes (coding)                    :: 5,320
            CDSs (with protein)               :: 5,320
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 237
            CDSs (without protein)            :: 237
            Pseudo Genes (ambiguous residues) :: 0 of 237
            Pseudo Genes (frameshifted)       :: 102 of 237
            Pseudo Genes (incomplete)         :: 130 of 237
            Pseudo Genes (internal stop)      :: 52 of 237
            Pseudo Genes (multiple problems)  :: 41 of 237
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2308
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267800-rid25106413.denovo.411"
                     /serotype="O 26:H11"
                     /isolate="2201S31241"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-01"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            complement(337..468)
                     /locus_tag="ACHOSC_RS23920"
                     /old_locus_tag="pgaptmp_004784"
                     /pseudo
     CDS             complement(337..468)
                     /locus_tag="ACHOSC_RS23920"
                     /old_locus_tag="pgaptmp_004784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612086.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
     gene            750..2108
                     /locus_tag="ACHOSC_RS23925"
                     /old_locus_tag="pgaptmp_004785"
     CDS             750..2108
                     /locus_tag="ACHOSC_RS23925"
                     /old_locus_tag="pgaptmp_004785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000823236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase-like activity of phytase family
                     protein"
                     /protein_id="HGU4550557.1"
                     /translation="MKRKIIPVLIGCTLSFSALAAQPTAERYVVSFPEGTHVNYAGAF
                     ASAFPNGLPVGIGSGLLFTGKQGDALTFATITDRGPNADSPKEGKNETKIFVTPDFAP
                     LLMTIRVQNGKAEAIDPRPLHDDKGAINGLPLASDVIGSTNEVAFSDTLHRLKGDNRG
                     LDTEGITPDGKGGYWLCDEYGPFLINIDSKGKILAIHGPQAAEGEKAIAGGLPNILKW
                     RQANRGFEGLTRMPDGRIIVAVQSTLDIDAKSKKKALFTRLVSFDPASGKTAMYGYPI
                     DSAAYSKNSDAKIGDIVALDNQHILLIEQGRDKNNRMRNLIYEVDLNKASDLSGFDKP
                     GEYPEFDDEKTLSQRGITLAQKTQVVDLRSLGWQQEKAEGLALIDSKTLAVANDNDFG
                     VKVAMQHPVEGKTFKDYRVNAEGKLTLDDKQVETTLRVKPLEKPESDSELWIVTLPEA
                     LK"
BASE COUNT          603 a          558 c          595 g          552 t
ORIGIN      
        1 catcaggtga tatttgagat tttacataaa agataatctc tcaaggagtc ttatctgaaa
       61 acgaaatata tagtccctct cgttttgtaa tatcgattgg ctttgttgaa tcggttacgt
      121 accccggagc cggtcatatt ttcaggtaat acacacgctt tcagacattt cggctttcgt
      181 cattttggta tcatgggcat agcttctgct acctgaccat cataatcacg cagcttcagt
      241 gaagcagtga ccacctgttt aacccggtgt atcgttatga tcttatccag caattgaacc
      301 gccccgggtt tcctggagag tgttttatct gtgaactcag gctgccagat catcgtttcc
      361 gatggaagca taataagctt tttctgcttc tgccggtgga atatggccct gcctttccag
      421 caatcgtcga ttgttatacc agtccaccca cgtgagtgtg gtcagctcat catactgaca
      481 cgtgcattac ccctaccgcg ataatgcaac cgtacaatat agggcgtttc ctaaacgcag
      541 ccctcatcac tccaatccaa caagacacag catttttgat cacgaagaaa gacaaataat
      601 ttccgtaaat gtttccgttg ttacaaataa caacagtgtt ttatatttct ttggcctcag
      661 gcccgcgcat gttgccattc gatgtcattt tcctgactcg tttcttcttt atggtgtagt
      721 gtgatcttca ctttatggat gctaacaata tgaaaagaaa aattattccc gtgctaatcg
      781 gttgtacgct ctctttctct gccctggcgg cacaacctac ggctgagcgc tatgtcgtca
      841 gttttccaga aggcacgcac gtgaactatg ccggcgcatt tgccagtgca ttcccgaacg
      901 gccttccggt tgggataggt tctggcctgc tgttcaccgg taaacagggc gatgccctga
      961 cattcgcaac cattaccgat cgcggtccga acgcggattc accgaaagag gggaaaaacg
     1021 aaactaaaat tttcgttacg ccagatttcg cgccgctgct gatgacgatt cgtgtacaaa
     1081 acggcaaagc ggaggccatc gatccccgtc ctctgcatga cgataaaggc gcgatcaacg
     1141 gtctgccgct ggcaagcgac gtgattggct ccaccaatga agtggccttc agcgacacgt
     1201 tacatagact gaagggggat aatcgcgggc tggatacgga agggatcact ccggatggta
     1261 aaggaggtta ctggctgtgc gatgagtatg gcccgttcct gattaacatc gacagtaaag
     1321 ggaaaatcct ggcaatccac ggtccgcagg cggcggaagg ggaaaaagcg attgcgggcg
     1381 gtttgccaaa tatcctcaaa tggcgtcagg cgaaccgcgg ctttgaaggc ctcacccgta
     1441 tgccggacgg gcgtattatt gtcgccgtgc aaagtacgct ggatatcgac gctaagagca
     1501 agaaaaaggc gctgtttacg cgtctggtga gcttcgatcc ggcaagcgga aaaaccgcga
     1561 tgtacggtta tcctatcgac agcgcggcct acagcaaaaa cagcgacgct aaaatcggag
     1621 acatcgtggc gctcgataac cagcacatcc tgctgatcga acagggaaga gataaaaaca
     1681 acagaatgcg taacctgatt tacgaggtcg atctcaataa ggcgagcgac ctgtcaggct
     1741 tcgacaagcc gggtgagtat ccggagtttg atgatgagaa aaccctgtca cagcgcggca
     1801 ttacccttgc gcagaaaacg caggttgttg atctgcgctc cttgggctgg cagcaggaga
     1861 aggctgaagg gttggcgctg atcgacagca aaacgctggc ggtggcgaac gataacgact
     1921 ttggcgtgaa ggtggcgatg caacatccgg tcgagggcaa gacattcaag gattatcggg
     1981 tgaacgcgga aggaaagctg acgcttgacg acaagcaggt tgaaacgacc ctcagagtga
     2041 agccactgga gaaaccggag tctgacagtg aactgtggat tgtgacgctg ccggaagcgt
     2101 tgaaataagt ttataaggta cggacagcag tatcgcttag tttttcaaat tcatgtatgt
     2161 gaacaatatt ggggttccgg cgtagacccc caatgaaagc gtcaccacgt gaaaacatgc
     2221 ctgcacggtt tacacgtcat ctcatggtgg cagccggtta actttcctgc tacaccagtg
     2281 gaaaaaggcc ataagcagtc cgaacaaa
//
LOCUS       DAZKHF020000410         2296 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2201S31241
            SAMN43267800-rid25106413.denovo.412, whole genome shotgun sequence.
ACCESSION   DAZKHF020000410 DAZKHF020000000
VERSION     DAZKHF020000410.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267800
            Sequence Read Archive: SRR30307263
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2296)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2296)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 2296)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350581.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350581.2
            Genome Coverage       :: 151x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:21:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,652
            CDSs (total)                      :: 5,557
            Genes (coding)                    :: 5,320
            CDSs (with protein)               :: 5,320
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 237
            CDSs (without protein)            :: 237
            Pseudo Genes (ambiguous residues) :: 0 of 237
            Pseudo Genes (frameshifted)       :: 102 of 237
            Pseudo Genes (incomplete)         :: 130 of 237
            Pseudo Genes (internal stop)      :: 52 of 237
            Pseudo Genes (multiple problems)  :: 41 of 237
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2296
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267800-rid25106413.denovo.412"
                     /serotype="O 26:H11"
                     /isolate="2201S31241"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-01"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            134..637
                     /locus_tag="ACHOSC_RS23930"
                     /old_locus_tag="pgaptmp_004786"
     CDS             134..637
                     /locus_tag="ACHOSC_RS23930"
                     /old_locus_tag="pgaptmp_004786"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HGU4550558.1"
                     /translation="MNNIAPQSPVMRRLTLQDNPAIARVIRQVSAEYGLTADKGYTVA
                     DPNLDELYQVYSQPGHAYWVVEYEGEVVGGGGIAPLAGSESDICELQKMYFLPAIRGK
                     GLAKKLALMAMEQAREMGFKRCYLETTAFLKEAIALYEHLGFEHIDYALGCTGHVDCE
                     VRMLREL"
     gene            complement(683..1099)
                     /gene="rraB"
                     /locus_tag="ACHOSC_RS23935"
                     /old_locus_tag="pgaptmp_004787"
     CDS             complement(683..1099)
                     /gene="rraB"
                     /locus_tag="ACHOSC_RS23935"
                     /old_locus_tag="pgaptmp_004787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease E inhibitor RraB"
                     /protein_id="HGU4550559.1"
                     /translation="MANPEQLEEQREETRLIIEELLEDGSDPDALYTIEHHLSADDLE
                     TLEKAAVEAFKLGYEVTDPEELEVEDGDIVICCDILSECALNADLIDAQVEQLMTLAE
                     KFDVEYDGWGTYFEDPNGEDGDDEDFVDEDDDGVRH"
     gene            1261..2274
                     /gene="argF"
                     /locus_tag="ACHOSC_RS23940"
                     /old_locus_tag="pgaptmp_004788"
     CDS             1261..2274
                     /gene="argF"
                     /locus_tag="ACHOSC_RS23940"
                     /old_locus_tag="pgaptmp_004788"
                     /EC_number="2.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004585 - ornithine carbamoyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0042450 - arginine biosynthetic process via
                     ornithine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine carbamoyltransferase"
                     /protein_id="HGU4550560.1"
                     /translation="MSGFYHKHFLKLLDFTPAELNSLLQLAAKLKADKKSGKEEAKLT
                     GKNIALIFEKDSTRTRCSFEVAAYDQGAHVTYLGPSGSQIGHKESIKDTARVLGRMYD
                     GIQYRGYGQEIVETLAEYAGVPVWNGLTNEFHPTQLLADLLTMQEHLPGKAFNEMTLV
                     YAGDARNNMGNSMLEAAALTGLDLRLVAPQACWPEAALVTECRALAQQNGGNITLTED
                     VAKGVEGADFIYTDVWVSMGEAKEKWAERIALLRDYQVNSKMMQLTGNPEVKFLHCLP
                     AFHDDQTTLGKKMAEEFGLHGGMEVTDEVFESAASIVFDQAENRMHTIKAVMVATLSK
                     LNN"
BASE COUNT          584 a          541 c          576 g          595 t
ORIGIN      
        1 ttctgccacg ttacaatact cttatcagta ataggctggc aaaaacttta ttgctttata
       61 agatgcttgc gcacgtaaga agagagtggt attaaacaaa tgtataatta ctttattttt
      121 attgggttaa actatgaata acattgcgcc gcaatcacct gtaatgcgtc gcctgacgct
      181 gcaggataat cctgctattg cccgcgtcat tcgtcaggta tccgccgaat acggtcttac
      241 cgctgataaa ggctacaccg tcgccgatcc gaatcttgac gagctgtatc aagtgtatag
      301 tcagccgggt catgcatatt gggtggttga gtacgagggt gaagtggtcg gcggcggtgg
      361 gattgcgcca ttagcaggta gtgaatcgga catttgcgaa ctgcaaaaga tgtattttct
      421 tccggctatt cgcggtaaag ggctggcgaa aaaactggcc ttaatggcga tggagcaggc
      481 acgagagatg ggcttcaaac gctgctatct ggaaacgacc gcttttttaa aggaagccat
      541 tgcgctttat gagcatttgg gctttgagca tatcgactat gcgcttggct gcacgggcca
      601 tgtcgattgt gaagtgcgga tgctacgcga actctaaaaa aatgcccgct agtgtgtgct
      661 atcgggcatt ttaatcaatt aattagtggc gaaccccgtc atcgtcttca tcgacaaaat
      721 cttcatcgtc gccgtcttcg ccgttgggat cttcaaagta agtgccccaa ccgtcgtact
      781 caacgtcaaa tttctccgcc agcgtcatca gttgttcaac ctgggcatcg atcagatcgg
      841 cattcaacgc gcactcgctg aggatgtcgc agcaaatcac gatatcacca tcttcaactt
      901 ccagctcttc tggatcggtc acttcgtaac cgagtttaaa cgcttcaact gctgcttttt
      961 ccagggtttc cagatcgtct gcggaaagat ggtgttcgat ggtatacagc gcgtccggat
     1021 cgctgccatc ttccagtaat tcttcaataa tcaaacgtgt ttcttcacgc tgttcttcca
     1081 gttgttccgg gtttgccatg gctcattcct cttaatgtcc tgccgatact cttattgtca
     1141 cacaccactg acattgcctc cacctttgtg acaaagattt atgctttaga cttgcaaatg
     1201 aataatcatc catataaatt gaattttaat tcattgaggt gttagccaca ggagggatct
     1261 atgtccgggt tttatcataa gcatttcctg aaattactcg atttcacgcc agctgaactc
     1321 aacagcctgc tgcagttagc cgcgaagctg aaagccgata agaaaagcgg caaagaagaa
     1381 gccaaactca ctggtaaaaa catcgcgctc atcttcgaaa aagactcgac ccgtacccga
     1441 tgctctttcg aagttgccgc atatgaccag ggtgctcacg ttacttatct cggcccaagc
     1501 ggcagtcaga ttggtcataa agagtcgatt aaagacactg cccgcgtgct gggtcgcatg
     1561 tatgacggta ttcagtatcg cggctatggt caggagattg tcgaaacact ggcggaatac
     1621 gctggcgtgc cggtatggaa cggcctgacc aatgagtttc accccacgca gttgctggcg
     1681 gatctcctca ccatgcagga gcatttaccc ggtaaagcat tcaacgaaat gacgctggtc
     1741 tatgcaggtg acgcacgtaa caacatgggc aattcgatgc tcgaagctgc ggcgcttacc
     1801 ggtctggatt tgcgtctggt cgcgccacag gcatgctggc cggaagctgc gctggttacg
     1861 gaatgccgcg ctctggcaca gcaaaatggc gggaatatta cgctgacgga agatgtagct
     1921 aaaggagttg aaggtgctga ctttatctat accgatgtat gggtgtcgat gggtgaagca
     1981 aaagagaaat gggctgaacg cattgcattg ctgcgtgatt atcaagtgaa cagcaagatg
     2041 atgcagttga ccggtaaccc ggaggtcaaa ttcctccact gcctgcccgc gtttcatgac
     2101 gaccaaacga cgcttggcaa gaaaatggcg gaagagtttg gcctacatgg cggaatggaa
     2161 gtgactgatg aggtcttcga atctgccgcc agcattgtat ttgatcaggc ggaaaaccgc
     2221 atgcatacta tcaaagcggt gatggtcgcg acgctcagta aattaaataa ttaatatctc
     2281 tatcaagatg ccggga
//
LOCUS       DAZKHF020000411         2257 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2201S31241
            SAMN43267800-rid25106413.denovo.413, whole genome shotgun sequence.
ACCESSION   DAZKHF020000411 DAZKHF020000000
VERSION     DAZKHF020000411.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267800
            Sequence Read Archive: SRR30307263
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2257)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2257)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 2257)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350581.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350581.2
            Genome Coverage       :: 151x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:21:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,652
            CDSs (total)                      :: 5,557
            Genes (coding)                    :: 5,320
            CDSs (with protein)               :: 5,320
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 237
            CDSs (without protein)            :: 237
            Pseudo Genes (ambiguous residues) :: 0 of 237
            Pseudo Genes (frameshifted)       :: 102 of 237
            Pseudo Genes (incomplete)         :: 130 of 237
            Pseudo Genes (internal stop)      :: 52 of 237
            Pseudo Genes (multiple problems)  :: 41 of 237
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2257
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267800-rid25106413.denovo.413"
                     /serotype="O 26:H11"
                     /isolate="2201S31241"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-01"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            complement(1..184)
                     /locus_tag="ACHOSC_RS23945"
                     /old_locus_tag="pgaptmp_004789"
                     /pseudo
     CDS             complement(1..184)
                     /locus_tag="ACHOSC_RS23945"
                     /old_locus_tag="pgaptmp_004789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309678.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tail fiber assembly protein"
     gene            334..1002
                     /locus_tag="ACHOSC_RS23950"
                     /old_locus_tag="pgaptmp_004790"
     CDS             334..1002
                     /locus_tag="ACHOSC_RS23950"
                     /old_locus_tag="pgaptmp_004790"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HGU4550561.1"
                     /translation="MVKLMKKNTDDGAKIYTPLTLKLYDWWVLGVSNRLAWGCPTKEH
                     LLPHFLEHLGNNHLDIGVGTGFYLTHVPESSLISLMDLNEASLNAASTRAGESKIKHK
                     ISHDVFEPYPAALHGQFDSISMFYLLHCLPGNISTKSCVIRNAAQALTDDGTLYGATI
                     LGDGVVHNSFGQKLMRIYNQKGIFSNTKDSEEGLTHILSEHFENVKTKVQGTVVMFSA
                     SGKK"
     gene            complement(947..1084)
                     /locus_tag="ACHOSC_RS23955"
                     /old_locus_tag="pgaptmp_004791"
     CDS             complement(947..1084)
                     /locus_tag="ACHOSC_RS23955"
                     /old_locus_tag="pgaptmp_004791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001309429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleocapsid protein"
                     /protein_id="HGU4550562.1"
                     /translation="MAYEPCQGVIIGIMPQHVLSKNVLRLDAIFSLKRKTLLQYLEPW
                     F"
     gene            complement(1057..1641)
                     /locus_tag="ACHOSC_RS23960"
                     /old_locus_tag="pgaptmp_004792"
     CDS             complement(1057..1641)
                     /locus_tag="ACHOSC_RS23960"
                     /old_locus_tag="pgaptmp_004792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000885581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0098004 - virus tail fiber assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tail fiber assembly protein"
                     /protein_id="HGU4550563.1"
                     /translation="MAFRMSEQARTIKIYNLLAGTNEFIGEGDAYIPPHTGLPANSTD
                     IAPPDIPAGFVAVFNSDEASWHLVEDHRGKTVYDVASGDALFISELGPLPENVTWLSP
                     EGEFQKWNGTAWVKDAEAEKLFRIREAEEIKNSLMQVASEHIAPLQDAVDLEIATEEE
                     TSLLEAWKKYRVLLNRVDTSTAPDIEWPTNPVRE"
     gene            complement(1641..>2257)
                     /locus_tag="ACHOSC_RS23965"
                     /old_locus_tag="pgaptmp_004793"
     CDS             complement(1641..>2257)
                     /locus_tag="ACHOSC_RS23965"
                     /old_locus_tag="pgaptmp_004793"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="tail fiber protein"
                     /protein_id="HGU4550564.1"
                     /translation="FDKSAYPKLAAAYPSGVIPDMRGWTIKGKPASGRAVLSQEQDGI
                     KSHTHSASASSTDLGTKTTSSFDYGTKSTNNTGAHTHSLSGSTSSAGAHSHVDGRRFN
                     TSTFKDTYQYGSTAVGSNSYQVQGAVGLGIGTMANTNSAGAHTHSLSGTAASAGAHAH
                     TVGIGAHTHSVAIGSHGHTITVNAAGNAENTVKNIAFNYIVRLA"
BASE COUNT          587 a          549 c          495 g          626 t
ORIGIN      
        1 taatgactgt tactgtaaca tgagttacaa acagcttcat acccagagct actgatgtat
       61 caactcgatt caacaatacc cgatatttat tccatgcctc cagcaacgat ctttcttcct
      121 ccgttgcgat ttccagatct acagcatcct gcagtggcgc aatatactca ctgaattcct
      181 ggatgtagaa ctgtgtggtg acggtcttcc agccattcgg ctcctgttgt atcgaagcat
      241 accaggctat ttcaatatcg ctatgctgcg gcagcattta accccttgta attcatcgcc
      301 ataattgatt taattcacaa ataaaactat aacatggtga aattaatgaa aaaaaacaca
      361 gatgatgggg ctaaaattta cacaccactt accctaaagc tttatgactg gtgggttttg
      421 ggagtatcaa atcggcttgc atggggatgt cctacaaagg aacaccttct tccacacttt
      481 ctggaacatt taggtaacaa ccatctggat attggtgttg gaactgggtt ttaccttact
      541 cacgtacctg agagtagtct gatatcttta atggatttga acgaagctag cctgaacgcg
      601 gcatctacaa gggctgggga atcaaaaatt aaacataaaa ttagccatga tgtttttgaa
      661 ccttatcccg cggcgttaca tggtcaattt gattccattt ccatgtttta ccttcttcac
      721 tgcctgcctg gaaatatatc tacaaaaagc tgtgtaatac gcaatgcggc gcaggcctta
      781 actgacgatg gaactctata cggagccaca attcttggcg atggagttgt gcacaatagc
      841 ttcggtcaaa aactgatgcg catttacaat cagaaaggca tcttttcaaa cacaaaagat
      901 tccgaagaag gcttaacaca tatactctca gagcatttcg agaatgttaa aaccaaggtt
      961 caaggtactg tagtaatgtt ttccgcttca gggaaaaaat agcatccaac cgcagcacgt
     1021 tcttgcttaa gacgtgctgc ggcataatcc caatgattac tccctgacag ggttcgtagg
     1081 ccactcaata tcaggtgcag ttgatgtatc aacacggttc agcaacaccc gatacttttt
     1141 ccaggcttcc agcaatgagg tttcttcctc cgttgcgatt tccagatcta cagcatcctg
     1201 aagtggcgca atatgctcac tggctacctg catcaggctg ttttttattt cttccgcctc
     1261 tcggatccgg aacagttttt ctgcttctgc atctttcacc caggctgtgc cgttccactt
     1321 ctgaaactcc ccttccggcg ataaccaggt aacattttcc ggtaacggac cgagttcaga
     1381 aataaataac gcgtcccctg acgctacgtc ataaaccgtt ttaccccgat ggtcttcaac
     1441 gagatgccac gatgcctcat cactgttgaa aacagccaca aagccagcag gaatatctgg
     1501 tggtgcaata tcggtactgt ttgctggcag acctgtatga ggcggaatat atgcatcacc
     1561 ttcaccaata aattcattag ttccggccag cagattataa atttttatgg tccgtgcttg
     1621 ttcactcatt ctgaatgcca ttatgcaagc ctcacaatat agttaaatgc gatgtttttg
     1681 acggtgtttt ccgcgttacc agcagcgtta acggtgatgg tgtgtccatg tgagccaatc
     1741 gcaacggagt gcgtatgagc accaataccg acagtatgtg cgtgtgcacc tgcgcttgca
     1801 gcagtgccgg acagtgagtg ggtatgcgcg ccagcagagt tcgtattcgc catcgtgcca
     1861 atgcccagac caaccgcccc ttgtacctgg tatgagttac ttccgacagc tgtagaacca
     1921 tactgatagg tgtctttaaa agtgcttgta ttaaacctgc gaccatcaac gtgcgagtgt
     1981 gctccggccg agcttgtaga gccgctcaga ctgtgcgtat gagccccggt gttattcgtg
     2041 gatttagtgc cgtaatcaaa cgacgatgtg gttttcgtcc ccaaatccgt actggatgcg
     2101 ctggcgctgt gggtgtgcga tttaatgccg tcctgttcct gagacaatac ggcacgacca
     2161 ctggcgggtt tgcccttaat cgtccagcca cgcatatcag ggatcacgcc tgacggataa
     2221 gcggctgcaa gtttcgggta tgcagatttg tcaaaag
//
LOCUS       DAZKHF020000412         2240 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2201S31241
            SAMN43267800-rid25106413.denovo.414, whole genome shotgun sequence.
ACCESSION   DAZKHF020000412 DAZKHF020000000
VERSION     DAZKHF020000412.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267800
            Sequence Read Archive: SRR30307263
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2240)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2240)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 2240)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350581.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350581.2
            Genome Coverage       :: 151x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:21:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,652
            CDSs (total)                      :: 5,557
            Genes (coding)                    :: 5,320
            CDSs (with protein)               :: 5,320
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 237
            CDSs (without protein)            :: 237
            Pseudo Genes (ambiguous residues) :: 0 of 237
            Pseudo Genes (frameshifted)       :: 102 of 237
            Pseudo Genes (incomplete)         :: 130 of 237
            Pseudo Genes (internal stop)      :: 52 of 237
            Pseudo Genes (multiple problems)  :: 41 of 237
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2240
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267800-rid25106413.denovo.414"
                     /serotype="O 26:H11"
                     /isolate="2201S31241"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-01"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            complement(19..393)
                     /locus_tag="ACHOSC_RS23970"
                     /old_locus_tag="pgaptmp_004794"
     CDS             complement(19..393)
                     /locus_tag="ACHOSC_RS23970"
                     /old_locus_tag="pgaptmp_004794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001405127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4550565.1"
                     /translation="MEFKDLPMPFQEMAANVVRSQLATLDLSTVEKETIDNISGNVRR
                     AFIGLYEEKQLSDNQDLHEKYFLELMDIINKGFGLLMKKKGIRIAPLENHFTASSINS
                     CDLKHHTSDGKVESNNKISINH"
     gene            complement(416..634)
                     /gene="ydfC"
                     /locus_tag="ACHOSC_RS23975"
                     /old_locus_tag="pgaptmp_004795"
     CDS             complement(416..634)
                     /gene="ydfC"
                     /locus_tag="ACHOSC_RS23975"
                     /old_locus_tag="pgaptmp_004795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310310.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YdfC"
                     /protein_id="HGU4550566.1"
                     /translation="MQKREPVIIAPDYTDDELYEWMHQKIKAAQDLKWANEARAKQAE
                     NLSALEQDITRLEKAAALSIARMITYPR"
     gene            complement(664..792)
                     /gene="ydfB"
                     /locus_tag="ACHOSC_RS23980"
                     /old_locus_tag="pgaptmp_004796"
     CDS             complement(664..792)
                     /gene="ydfB"
                     /locus_tag="ACHOSC_RS23980"
                     /old_locus_tag="pgaptmp_004796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000344964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YdfB"
                     /protein_id="HGU4550567.1"
                     /translation="MDFNTIMEKAYEEYFEGLAEGEEALSFSEFKQALSSSAKSNG"
     gene            complement(794..949)
                     /locus_tag="ACHOSC_RS23985"
                     /old_locus_tag="pgaptmp_004797"
     CDS             complement(794..949)
                     /locus_tag="ACHOSC_RS23985"
                     /old_locus_tag="pgaptmp_004797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1391 family protein"
                     /protein_id="HGU4550568.1"
                     /translation="MDTIDLGNSESLVCGVFPNQDGTFTAMTYTRSKTFKTENGARRW
                     LERNSGE"
     gene            complement(1222..1938)
                     /locus_tag="ACHOSC_RS23990"
                     /old_locus_tag="pgaptmp_004798"
     CDS             complement(1222..1938)
                     /locus_tag="ACHOSC_RS23990"
                     /old_locus_tag="pgaptmp_004798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000103684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S24 family peptidase"
                     /protein_id="HGU4550569.1"
                     /translation="MSTIATSLGSNIGYMKNTISERIRNRRKDVGLTQQQVAKAIGIS
                     RVSVTKWENGSSKPDGENLYLLSKLLSKSPEWILYGKDGHDKTDDLRLNQYPYISDNI
                     ARLPVLTWEQAGYWDMSCPVTKIPGIKNWVDVMTKTAENSFLLHVEGDAMTNSNGLPT
                     IPDGSTVLITPCSSNIRELVGKIILIQLEGTPNVTLKKVAIDGPNIYLLSLNPLYKPI
                     ELNGGYTIKGKVSQIHQYLD"
     gene            1988..2203
                     /locus_tag="ACHOSC_RS23995"
                     /old_locus_tag="pgaptmp_004799"
     CDS             1988..2203
                     /locus_tag="ACHOSC_RS23995"
                     /old_locus_tag="pgaptmp_004799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000471546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cro/CI family transcriptional regulator"
                     /protein_id="HGU4550570.1"
                     /translation="MFKDDVLRYFKKKRLVAEALGISHVAVVRWKAVIPKLRAMELDE
                     ITNGELKYNPELYKKQDSTSNEGKNDS"
BASE COUNT          565 a          483 c          490 g          702 t
ORIGIN      
        1 acctcacctg tgaataaatt aatgattaat tgatattttg ttgtttgatt caactttccc
       61 atcggatgtg tgatgcttta aatcacagga attaatactg cttgctgtaa aatgattttc
      121 aaggggagct attcgaatcc ctttcttttt cattaacaag ccaaatcctt tattaatgat
      181 gtccattaat tccagaaagt atttttcatg taaatcctgg ttatcagaga gctgcttctc
      241 ttcgtacagc ccgataaagg ctcggcgtac gttaccagat atattgtcga tggtttcttt
      301 ttctacggta ctcaggtcaa gagtcgccag ttgagagcga accacattcg ctgccatttc
      361 ctggaatggc attggtaaat ctttaaattc cattattagc ctcgttggtt agctattaac
      421 gcgggtatgt aatcattctg gcaatgctta atgccgctgc tttttccagc ctggtgatat
      481 cctgctccag agcggacaga ttttcagcct gcttagccct ggcttcattg gcccatttca
      541 ggtcctgcgc agccttaatt ttctggtgca tccactcata aagttcatca tcggtatagt
      601 ctggcgcgat gatgacgggt tctcgtttct gcatactgat tcctcgcggt gctgtttcgc
      661 ttatcagccg ttagattttg ccgaactgga aagcgcctgt ttaaactcac tgaagctgag
      721 ggcttcttcg ccttcggcaa ggccttcgaa gtattcttcg taagcctttt ccatgattgt
      781 gttgaaatcc atatcactca cctgagtttc tttccagcca gcgacgggca ccattttcgg
      841 ttttaaacgt tttgcttctg gtatacgtca tcgcggtgaa cgtaccgtcc tggttgggga
      901 acacgccaca taccagagat tcgctgttgc caagatcgat agtatccatg ctgacctcat
      961 ttccccttaa cgccggggta gcggaacaaa aacctgctgc atagttatta aagttgaacc
     1021 ctgccgtcat gttcttacgc ctcgggctgg ctacttaacc cctgatcact gccgggtaac
     1081 tcgaagtatt tccctgcgtt ctgtggggcg gggtgggttg gtatgttgtt aaggtaacaa
     1141 gagttacctt tcgagtcaat acaatgttgc aaaaggtaca tttgagggca taaaaaaccc
     1201 gcaatgaatg cgggttctga ctcagtctaa gtattgatgt atttgtgaaa ctttaccttt
     1261 aatggtgtaa ccaccattca gttcgatggg tttgtaaagc ggattcagtg acaacagata
     1321 gatgtttggt ccgtcaatcg caactttttt tagtgttacg tttggcgttc cttccaattg
     1381 gattaagatt atttttccca ccagttctct aatgttactt gagcatggtg tgatcagcac
     1441 ggtagatccg tcggggatgg ttgggaggcc gttagagttt gtcatcgcat ctccctcaac
     1501 atgcaataaa aaagagtttt cagcggtttt tgtcatgaca tcaacccagt tcttaatacc
     1561 aggaatcttg gttactggac aactcatatc ccaataacca gcctgttccc acgttaaaac
     1621 gggcaaccgg gcgatgttgt cactaatgta agggtactga ttcagacgca gatcatcggt
     1681 tttatcgtga ccgtcctttc cataaagaat ccattcagga gatttggaaa gcaattttga
     1741 cagtagatac aaattctcac cgtcaggttt tgaagagcca ttttcccatt ttgttacgga
     1801 tacacgagat atgccgattg ctttcgcaac ctgctgttgg gttaatccaa cgtcttttcg
     1861 acgattccga atacgttcgc tgatagtgtt tttcatgtaa ccaatgttac taccaagtga
     1921 tgttgctatg gttgacattg ttatgtaact attgttaccc ttctgctcga aataacagga
     1981 gagttttatg ttcaaagatg atgttctgcg ctatttcaaa aaaaagcgac tagtagctga
     2041 ggctcttgga atttcacatg tggctgttgt gcggtggaaa gcagttattc ccaaacttcg
     2101 cgcaatggaa ctggatgaaa ttactaacgg tgaattgaaa tacaacccag aactttacaa
     2161 gaagcaggat agcacctcga acgaaggaaa gaatgattca tgaaaatcaa gcatgaacac
     2221 atccgcatgg cgatgaatgt
//
LOCUS       DAZKHF020000413         2239 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2201S31241
            SAMN43267800-rid25106413.denovo.415, whole genome shotgun sequence.
ACCESSION   DAZKHF020000413 DAZKHF020000000
VERSION     DAZKHF020000413.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267800
            Sequence Read Archive: SRR30307263
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2239)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2239)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 2239)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350581.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350581.2
            Genome Coverage       :: 151x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:21:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,652
            CDSs (total)                      :: 5,557
            Genes (coding)                    :: 5,320
            CDSs (with protein)               :: 5,320
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 237
            CDSs (without protein)            :: 237
            Pseudo Genes (ambiguous residues) :: 0 of 237
            Pseudo Genes (frameshifted)       :: 102 of 237
            Pseudo Genes (incomplete)         :: 130 of 237
            Pseudo Genes (internal stop)      :: 52 of 237
            Pseudo Genes (multiple problems)  :: 41 of 237
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2239
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267800-rid25106413.denovo.415"
                     /serotype="O 26:H11"
                     /isolate="2201S31241"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-01"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            137..469
                     /gene="hdeA"
                     /locus_tag="ACHOSC_RS24000"
                     /old_locus_tag="pgaptmp_004800"
     CDS             137..469
                     /gene="hdeA"
                     /locus_tag="ACHOSC_RS24000"
                     /old_locus_tag="pgaptmp_004800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid-activated periplasmic chaperone HdeA"
                     /protein_id="HGU4550571.1"
                     /translation="MKKVLGVILGGLLLLPVVSNAADAQKAADNKKPVNSWTCEDFLA
                     VDESFQPTAVGFAEALNNKDKPEDAVLDVQGIATVTPAIVQACTQDKQANFKDKVKGE
                     WDKIKKDM"
     gene            585..911
                     /gene="hdeB"
                     /locus_tag="ACHOSC_RS24005"
                     /old_locus_tag="pgaptmp_004801"
     CDS             585..911
                     /gene="hdeB"
                     /locus_tag="ACHOSC_RS24005"
                     /old_locus_tag="pgaptmp_004801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417966.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid-activated periplasmic chaperone HdeB"
                     /protein_id="HGU4550572.1"
                     /translation="MNISSLRKAFIFMGAVAALSLVNAQSALAANESAKDMTCQEFID
                     LNPKAMTPVAWWMLHEETVYKGGDTVTLNETDLTQIPKVIEYCKKNPQKNLYTFKNQA
                     SNDLPN"
     gene            975..1622
                     /locus_tag="ACHOSC_RS24010"
                     /old_locus_tag="pgaptmp_004802"
     CDS             975..1622
                     /locus_tag="ACHOSC_RS24010"
                     /old_locus_tag="pgaptmp_004802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MgtC/SapB family protein"
                     /protein_id="HGU4550573.1"
                     /translation="MTAEFIIRLILAAIACGAIGMERQMRGKGAGLRTHVLIGMGSAL
                     FMIVSKYGFADVLSLDHVGLDPSRIAAQVVTGVGFIGAGNILVRNQNIVGLTTAADIW
                     VTAAIGMVIGSGMYELGIYGSVMTLLVLEVFHQLTFRLMNKNYHLQLTLVNGNTVSML
                     DWFKQQKIKTDLVSLQENEDHEVVAIDIQLHATTSIEDLLRLLKGIAGVKGVSIS"
     gene            complement(1664..2194)
                     /locus_tag="ACHOSC_RS24015"
                     /old_locus_tag="pgaptmp_004803"
     CDS             complement(1664..2194)
                     /locus_tag="ACHOSC_RS24015"
                     /old_locus_tag="pgaptmp_004803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709290.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LuxR C-terminal-related transcriptional
                     regulator"
                     /protein_id="HGU4550574.1"
                     /translation="MFLIITRDTMFFTAMKNILSKGNVVHIQNEEEIDVMLHQNAFVI
                     IDTLMNNVFHSNFLTQIERLKPVHVIVFSPFNIKRCLGKVPVTFVPRTITIIDFVALI
                     NGSYCSVPEANVSLSRKQHQVLSCIANQMTTEDILEKLKISLKTFYCHKHNIMMILNL
                     KRINELVRHQHIDYLV"
BASE COUNT          670 a          432 c          496 g          641 t
ORIGIN      
        1 aactcattgt attgaaataa aaatatctga ttttgatatt ttccatcaac atgacatata
       61 cagaaaacca ggttataacc tcagtgtcga aattgattcg tgacggctct ttcactttat
      121 agttgaggat attacgatga aaaaagtatt aggcgttatt cttggtggtc tgcttcttct
      181 gccagttgtg agcaatgcag cggatgcgca aaaagcagct gataacaaaa aaccggtcaa
      241 ctcctggacc tgtgaagatt tcctggctgt ggacgaatcc ttccagccaa ctgcagttgg
      301 ttttgctgaa gcgctgaaca acaaagataa accagaagat gcggttttag atgttcaggg
      361 tattgcaacc gtaaccccag ctatcgttca ggcttgtact caggataaac aagccaactt
      421 taaagataaa gttaaaggcg aatgggacaa aattaagaaa gatatgtaat tccgggaatg
      481 cgttacatcg tacttccttg catattgaac aggccggaat atcttcttta aaagcagcta
      541 ttcctcctgt tcatatataa tctctatatt gaatgggtta caaaatgaat atttcatctc
      601 tccgtaaagc gtttattttt atgggcgctg tagcggcttt gtcactggtg aacgcacaat
      661 ctgcgctggc agccaatgaa tccgctaaag atatgacctg ccaggaattt attgatctga
      721 atccaaaagc aatgaccccg gttgcatggt ggatgctgca tgaagaaaca gtatataaag
      781 gtggcgatac cgttacttta aatgaaaccg atctcactca aattcctaaa gtgatcgaat
      841 actgtaagaa aaacccgcag aaaaatttgt ataccttcaa aaatcaagca tctaatgact
      901 tgccgaatta atgaggtaca agtaaaaagg agtagcaagt tgagccatct tgctgctcct
      961 ttttgcattt ttatatgaca gcagaattta ttatacgtct tatactcgcg gcaattgcct
     1021 gtggcgctat tggcatggaa aggcaaatgc gcggcaaagg agcagggtta cgcacacatg
     1081 tattaattgg catgggaagc gccctgttta tgattgtttc gaaatatggt tttgctgacg
     1141 tgctgtcttt agatcacgtc ggactcgacc ccagccgtat tgctgctcag gtggtgacgg
     1201 gcgtcgggtt tatcggtgca ggtaacattc tcgttcgtaa ccaaaatatt gtaggtctga
     1261 cgacggcagc ggatatctgg gtgaccgccg ccataggtat ggttattggc agcggtatgt
     1321 acgaactggg tatttatggt tcagtgatga ccttgttggt gctggaagtc ttccatcaat
     1381 taaccttccg cctgatgaac aaaaattacc atttacagct gacattagtg aacggtaata
     1441 cggtatcaat gctcgactgg tttaagcagc aaaaaataaa aacggattta gtttcgttgc
     1501 aggaaaatga agatcatgaa gtggtcgcca ttgatatcca gcttcatgcc acaacgtcga
     1561 tagaagacct gttgcgatta ttaaaaggta tagcaggggt gaaaggcgtt tcaattagct
     1621 aatctgtgtg gcagcgtagc cagactcacc gtaagcctga aattcacacc agataatcaa
     1681 tatgctgatg gcgtaccagc tcattgatcc gcttaagatt gaggatcatc atgatattgt
     1741 gtttatggca gtagaacgtt tttagcgata ttttcagttt ctccagaata tcttcagttg
     1801 tcatttgatt cgcaatgcag ctcagaacct gatgttgctt gcgcgaaagt gacacattcg
     1861 cttcaggcac agagcagtaa ctgccattga tgagtgcgac aaaatcaatg atagtgatag
     1921 tccgcggaac aaaggtcacc ggtactttcc ccagacagcg tttaatatta aagggggaga
     1981 aaacaatgac atggacaggt tttaatcgtt caatttgagt gagaaaatta gaatgaaata
     2041 cattattcat taatgtatca ataatgacga aggcattctg atgcaacatt acgtcgatct
     2101 cttcttcgtt ctgtatatga acgacattac ctttactcag aatgtttttc atcgcggtga
     2161 agaacatcgt atccctggta attataagaa acataaccga ctcctggtgc ggacattaac
     2221 tttataatca gtttgccaa
//
LOCUS       DAZKHF020000414         2231 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2201S31241
            SAMN43267800-rid25106413.denovo.416, whole genome shotgun sequence.
ACCESSION   DAZKHF020000414 DAZKHF020000000
VERSION     DAZKHF020000414.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267800
            Sequence Read Archive: SRR30307263
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2231)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2231)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 2231)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350581.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350581.2
            Genome Coverage       :: 151x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:21:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,652
            CDSs (total)                      :: 5,557
            Genes (coding)                    :: 5,320
            CDSs (with protein)               :: 5,320
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 237
            CDSs (without protein)            :: 237
            Pseudo Genes (ambiguous residues) :: 0 of 237
            Pseudo Genes (frameshifted)       :: 102 of 237
            Pseudo Genes (incomplete)         :: 130 of 237
            Pseudo Genes (internal stop)      :: 52 of 237
            Pseudo Genes (multiple problems)  :: 41 of 237
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2231
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267800-rid25106413.denovo.416"
                     /serotype="O 26:H11"
                     /isolate="2201S31241"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-01"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            327..518
                     /locus_tag="ACHOSC_RS24020"
                     /old_locus_tag="pgaptmp_004804"
     CDS             327..518
                     /locus_tag="ACHOSC_RS24020"
                     /old_locus_tag="pgaptmp_004804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4222 domain-containing protein"
                     /protein_id="HGU4550575.1"
                     /translation="MSEDVPLPKVNQRYKDDHGALVTLTSVEETRVVFMQDGYPHPCM
                     RPMYNFLGKFKPEPREETE"
     gene            complement(571..804)
                     /locus_tag="ACHOSC_RS24025"
                     /old_locus_tag="pgaptmp_004805"
     CDS             complement(571..804)
                     /locus_tag="ACHOSC_RS24025"
                     /old_locus_tag="pgaptmp_004805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4550576.1"
                     /translation="MARSDYDIINLSLEHELNEWLAERGYAGLVDNRNRLAEVVTRKL
                     QDSFYINVSRDALNTAYSEHPEWFSGLVSGDEN"
     gene            complement(900..1523)
                     /locus_tag="ACHOSC_RS24030"
                     /old_locus_tag="pgaptmp_004806"
     CDS             complement(900..1523)
                     /locus_tag="ACHOSC_RS24030"
                     /old_locus_tag="pgaptmp_004806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HGU4550577.1"
                     /translation="MRTRIDYLADKYCFTERNESPRLRRQWQDVLEECRQTEAGPEER
                     LRIALLNVDYVTSFELPFRLLLTRTPQLIAALREEWGISQKNVVFNDKRFGCVYSLKA
                     SLSGVPDTYRYHLSHRIRRVVGNENTSLPYQQVAREVKAPRERLKYALEAGLLVTALD
                     GLFWFGSQRIAADVLRLRKAGMPVVTTTVEVHDNLTGTTRKVPAYHL"
     gene            complement(1612..2121)
                     /locus_tag="ACHOSC_RS24035"
                     /old_locus_tag="pgaptmp_004807"
     CDS             complement(1612..2121)
                     /locus_tag="ACHOSC_RS24035"
                     /old_locus_tag="pgaptmp_004807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4550578.1"
                     /translation="MQITEALISEPGEIRRFVQQAVDHWPNLLAFHFTLYSAEGIYGQ
                     QIQTFCSSFHRRVHERITEHNHTVSPSAPVVLRWLREQHEGAQIRCLLLLSQTSICHP
                     RVGVMADEECAQLVDLLQQTWSVISAGGQCRVERCFRVARPGSSGQYVALKTAVQSFM
                     SQVIATIIR"
BASE COUNT          630 a          581 c          483 g          537 t
ORIGIN      
        1 cagcacatta caaaatttat aaccataatt actggaaaag tgcagaaaat tattccactg
       61 aatgctttta atacagttgg ttagcttaaa aaatagcaat ttttgatttc agtcattgaa
      121 agttgcaatg tttgacgatt caacagcatt taagtgagtt tcaggatatg ggaaggacat
      181 gaaaggagga gcgcaataaa atacacagga agaaggggaa aatgtttcgt aaaaacagaa
      241 aattaacagc gcacatcagg aaacgcagtc tcctgattca gtgctaccac atgaaaattt
      301 agttaactgt taccgtacta aaatttatgt cagaagatgt tccattaccg aaagtcaatc
      361 agcgatacaa agacgatcat ggtgcgcttg tcaccctgac cagtgttgag gaaacccggg
      421 tggtgtttat gcaggacggc tatccgcatc cctgcatgcg accgatgtac aactttctgg
      481 gaaaattcaa acccgaaccc cgggaagaaa cagaataaaa cgaaatccct gccggcaatc
      541 acactacctg atagcagcat aatccactta ttaattctca tccccggaga caagcccaga
      601 aaaccactcc gggtgttcac tgtatgccgt gttcagcgca tccctggaaa cgtttatata
      661 aaagctgtcc tgcaatttgc gggtaacaac ttctgccagg cgattccggt tatccaccag
      721 tccggcataa cctctctctg ccagccactc attcagttca tgttccagag acagattgat
      781 aatatcataa tcggaccttg ccattcccgt ttccttagca catgttttca ggcacgggga
      841 taaagtataa agaatgtcat actgctgctg ccagagcaat attccccctg atggttgtat
      901 cagagatggt atgccggtac tttgcgggtt gttcccgtca ggttatcgtg cacctccacg
      961 gtcgtggtca ccaccggcat tccggccttt ctcagcctca gaacatcagc cgcaatacgc
     1021 tgactgccaa accagaacag gccgtccagt gcagtcacca gtaaacccgc ctccagtgca
     1081 tacttcagcc gttcacgggg cgctttcact tcccgggcaa cctgctgata tggcaatgat
     1141 gtgttttcat tcccaaccac gcgacgaata cggtgagaca gatgataccg gtatgtatcc
     1201 ggcacaccag aaaggctggc cttcaggctg tacacgcagc caaaccgttt atcgttaaac
     1261 accacatttt tctggctgat gccccattct tcacgcagcg cggcaatcaa ttgcggtgta
     1321 cgggtaagca gcaagcgaaa aggcagttca aaactggtga cgtaatccac attcagcagt
     1381 gcaatacgca accgttcttc tggcccggcc tctgtctgcc ggcactcctc cagaacatcc
     1441 tgccactgcc ggcgaagacg gggggattcg ttccgttctg taaaacagta tttatccgcc
     1501 agatagtcaa ttcgtgtacg catactgaaa ggaattccgt ataaagattc agcagacaaa
     1561 actttatcag ccagtaaaaa ttaacggaag cattgatatt cctcccgaca atcaccggat
     1621 gattgttgca ataacctgcg acataaagga ctgaacagcc gtttttaacg ccacgtattg
     1681 ccctgatgaa ccaggccggg ccacccgaaa acatctttcc acccggcatt gtccgccagc
     1741 acttatcacc gaccaggtct gttgcagtaa atccaccagt tgcgcacatt cttcatcagc
     1801 catgacgccg actcgcggat ggcaaatact cgtctggctg agcagcaaca gacatcgtat
     1861 ctgtgctcct tcatgttgtt cccgcaacca gcgtaatacc accggcgctg atgggctgac
     1921 cgtgtgatta tgctccgtaa tacgttcgtg tacccgtcgg tgaaaggaac tacaaaatgt
     1981 ctgaatctgt tgaccataga ttccttctgc cgaatagagc gtgaagtgaa atgccaacag
     2041 atttggccag tggtccaccg cctgctgaac aaaacgccgg atttcccccg gctctgaaat
     2101 taaggcttcg gttatttgca cgaaatgttc cttttcaacc cctgttgaat tttgtcaagc
     2161 cggtgcagac acagtaaagc gcgcatcaac acctgaaagg tacgatacaa acacaagtta
     2221 caaacaataa g
//
LOCUS       DAZKHF020000415         2216 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2201S31241
            SAMN43267800-rid25106413.denovo.417, whole genome shotgun sequence.
ACCESSION   DAZKHF020000415 DAZKHF020000000
VERSION     DAZKHF020000415.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267800
            Sequence Read Archive: SRR30307263
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2216)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2216)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 2216)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350581.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350581.2
            Genome Coverage       :: 151x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:21:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,652
            CDSs (total)                      :: 5,557
            Genes (coding)                    :: 5,320
            CDSs (with protein)               :: 5,320
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 237
            CDSs (without protein)            :: 237
            Pseudo Genes (ambiguous residues) :: 0 of 237
            Pseudo Genes (frameshifted)       :: 102 of 237
            Pseudo Genes (incomplete)         :: 130 of 237
            Pseudo Genes (internal stop)      :: 52 of 237
            Pseudo Genes (multiple problems)  :: 41 of 237
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2216
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267800-rid25106413.denovo.417"
                     /serotype="O 26:H11"
                     /isolate="2201S31241"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-01"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            117..641
                     /gene="aroL"
                     /locus_tag="ACHOSC_RS24040"
                     /old_locus_tag="pgaptmp_004808"
     CDS             117..641
                     /gene="aroL"
                     /locus_tag="ACHOSC_RS24040"
                     /old_locus_tag="pgaptmp_004808"
                     /EC_number="2.7.1.71"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004765 - shikimate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate kinase AroL"
                     /protein_id="HGU4550579.1"
                     /translation="MTQPLFLIGPRGCGKTTVGMALADSLNRRFVDTDQWLQSQLNMT
                     VAEIVEREEWAGFRARETAALEAVTAPSTVIATGGGIILTEFNRHFMQNNGIVVYLCA
                     PVSVLVNRLQAAPEEDLRPTLTGKPLSEEVQEVLEERDALYREVAHIIIDATNEPSQV
                     ISEIRSALAQTINC"
     gene            691..882
                     /gene="yaiA"
                     /locus_tag="ACHOSC_RS24045"
                     /old_locus_tag="pgaptmp_004809"
     CDS             691..882
                     /gene="yaiA"
                     /locus_tag="ACHOSC_RS24045"
                     /old_locus_tag="pgaptmp_004809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YaiA"
                     /protein_id="HGU4550580.1"
                     /translation="MPTKPPYPREAYIVTIEKGKPGQTVTWYQLRADHPKPDSLISEH
                     PTAQEAMDAKKRYEDPDKE"
     gene            1140..1817
                     /locus_tag="ACHOSC_RS24050"
                     /old_locus_tag="pgaptmp_004810"
     CDS             1140..1817
                     /locus_tag="ACHOSC_RS24050"
                     /old_locus_tag="pgaptmp_004810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AroM family protein"
                     /protein_id="HGU4550581.1"
                     /translation="MSASLAILTIGIVPMQEVLPLLTEYIDEDNISHHSLLGKLSREE
                     VMAEYAPEAGEDTILTLLNDNQLAHVSRRKVERDLQGVVEVLDNQGYDVILLMSTANI
                     SSMTARNTIFLEPSRILPPLVSSIVEDHQVGVIVPVEEMLTVQAQKWQILQKSPVFSL
                     GNPIHDSEQKIIDAGKELLAKGADVIMLDCLGFHQRHRDLLQKQLDVPVLLSNVLIAR
                     LAAELLV"
     gene            1889..2173
                     /gene="ppnP"
                     /locus_tag="ACHOSC_RS24055"
                     /old_locus_tag="pgaptmp_004811"
     CDS             1889..2173
                     /gene="ppnP"
                     /locus_tag="ACHOSC_RS24055"
                     /old_locus_tag="pgaptmp_004811"
                     /EC_number="2.4.2.1"
                     /EC_number="2.4.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine/purine nucleoside phosphorylase"
                     /protein_id="HGU4550582.1"
                     /translation="MLQSNEYFSGKVKSIGFSSSSTGRASVGVMVEGEYTFSTAEPEE
                     MTVISGALNVLLPDATDWQVYEAGSVFNVPGHSEFHLQVAEPTSYLCRYL"
BASE COUNT          588 a          497 c          560 g          571 t
ORIGIN      
        1 ttcactttaa gtggaatttt ttctttacaa tcgaaattgt actagtttga tggcatgatc
       61 gctattctca tgacaccggc tttcgccgca ttgcgaccta ttggggaaaa cccacgatga
      121 cacaacctct ttttctgatc gggcctcggg gctgtggtaa aacaacggtc ggaatggccc
      181 ttgccgattc gcttaaccgt cggtttgtcg ataccgatca gtggttgcaa tcacagctca
      241 atatgacggt cgcggagatc gtcgaaaggg aagagtgggc gggatttcgc gccagagaaa
      301 cggcggcgct ggaagcggta actgcgccat ccaccgttat cgctacaggc ggcggcatta
      361 ttctgacgga atttaatcgt cacttcatgc aaaataacgg gatcgtggtt tatttgtgtg
      421 cgccagtatc agtcctggtt aaccgactgc aagctgcacc ggaagaagat ttacggccaa
      481 ccttaacggg aaaaccgctg agcgaagaag ttcaggaagt gctggaagaa cgcgatgcgc
      541 tatatcgcga agttgcgcat attatcatcg acgcaacaaa cgaacccagc caggtgattt
      601 ctgaaattcg tagcgccctg gcacagacga tcaattgttg attttagagc gcctatactt
      661 aacgttcatc ccgtgaaata aggaagaacg atgccaacga aaccgcctta tcctcgtgaa
      721 gcatatatag tgacgattga aaaaggaaag ccaggacaga cggtaacctg gtaccaactc
      781 agagccgatc atcctaaacc agactcgttg atcagtgaac atccgacggc tcaggaagcg
      841 atggatgcga aaaaacgcta tgaggaccct gacaaagagt gaccgcatca gactgctcgg
      901 aagggattct gagtgccact acaagggatc tgcgtcacat ttttcataat tcatgttttt
      961 ctaataatta gaatattaaa caataacaat ccattactgg aatcatttgg aatctttaca
     1021 ttatgccgtg cacgtctgct gctacgcttt ttgtcatttg tagcacaagt aagtgtcagc
     1081 agtggtgctt cacacttgcc cggtaattaa cgacgaaaga aaagtaaggt ggatgaacaa
     1141 tgagtgcgtc gttggcgatc ctgaccatcg gcattgtacc tatgcaggag gttttgccgc
     1201 tcctgacgga atacattgac gaagataata tttcccatca tagcttgctg gggaagttaa
     1261 gtcgtgaaga agtgatggcg gagtacgcgc cagaagcagg cgaagacacc attctcacat
     1321 tattaaatga caaccagctg gcccatgttt cgcgtcgtaa agtggagcga gacctgcaag
     1381 gtgtggttga agtgctcgat aatcagggtt atgacgtcat tttattaatg agtacagcaa
     1441 acattagtag tatgactgcg cgtaatacga tctttcttga gccgtcgcga atattgcctc
     1501 cactggtttc ctctattgtt gaagatcatc aggtgggggt tatcgttccg gttgaggaga
     1561 tgctgaccgt tcaggcgcaa aaatggcaaa ttttgcagaa atcgccggta ttttcattgg
     1621 gcaaccccat tcatgattca gaacaaaaaa tcattgatgc cgggaaagaa ttactggcaa
     1681 aaggggctga tgtcatcatg ctggattgtt taggatttca ccaacgtcat cgcgatttac
     1741 tgcaaaaaca gctcgatgtt cctgtcttgc tgtctaacgt attgattgca cggctggctg
     1801 cggaattact ggtgtaattt tgcgtgacag ccagcgcctc tggcccctat agtgaagtag
     1861 atgttcaact accagacagg gccagtttat gcttcaaagt aatgagtact tttccggcaa
     1921 agtgaaatca atcggctttt ccagcagcag cactggtcgc gccagcgtgg gtgttatggt
     1981 tgaaggcgaa tacaccttca gcaccgctga gccggaagag atgacagtaa tcagtggcgc
     2041 gctgaatgtg ttactgcctg acgcgaccga ctggcaggtg tatgaagccg gttcggtgtt
     2101 taatgttccc ggtcacagtg agtttcatct gcaagttgcc gaacccacct cttatctgtg
     2161 ccgctatctg taattcctcg ccttcccctt gaacgggagg gcatttttct gaaata
//
LOCUS       DAZKHF020000416         2176 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2201S31241
            SAMN43267800-rid25106413.denovo.418, whole genome shotgun sequence.
ACCESSION   DAZKHF020000416 DAZKHF020000000
VERSION     DAZKHF020000416.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267800
            Sequence Read Archive: SRR30307263
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2176)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2176)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 2176)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350581.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350581.2
            Genome Coverage       :: 151x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:21:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,652
            CDSs (total)                      :: 5,557
            Genes (coding)                    :: 5,320
            CDSs (with protein)               :: 5,320
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 237
            CDSs (without protein)            :: 237
            Pseudo Genes (ambiguous residues) :: 0 of 237
            Pseudo Genes (frameshifted)       :: 102 of 237
            Pseudo Genes (incomplete)         :: 130 of 237
            Pseudo Genes (internal stop)      :: 52 of 237
            Pseudo Genes (multiple problems)  :: 41 of 237
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2176
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267800-rid25106413.denovo.418"
                     /serotype="O 26:H11"
                     /isolate="2201S31241"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-01"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            <1..1139
                     /gene="escC"
                     /locus_tag="ACHOSC_RS24060"
                     /old_locus_tag="pgaptmp_004812"
     CDS             <1..1139
                     /gene="escC"
                     /locus_tag="ACHOSC_RS24060"
                     /old_locus_tag="pgaptmp_004812"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="type III secretion system LEE outer membrane
                     ring protein EscC"
                     /protein_id="HGU4550583.1"
                     /translation="SCNVRKITTFNSIEVRGVPECIKYITSLSESLDKEAQSKAKNKD
                     VVRVFKLNYASATDITYKYRDQNVVVPGVVSILKTMASNGSLPSTGKGAVERNGNLFD
                     NSVKISADPRLNAVVVKDREITMDIYRQLISELDIEQRQIEISVSIIDVDANDLQQLG
                     VNWSGTLNAGQGSIAFNSSTAQANLSSSVISNANNFMIRVNALQQNSKAKILSQPSII
                     TLNNMQAILDKNVTFYTKVSGEKVASLESITSGTLLRVTPRILDDNSNSLTGKRRERV
                     RLLLDIQDGNQSTNQSNAQDASSILPEVQNSEMTTEATLSAGESLLLGGFIQDKESSS
                     KDGIPLLSDIPVIGNLFSSTTKQKHSVVRLFLIKATPIKSASSE"
     gene            1153..1608
                     /gene="cesD"
                     /locus_tag="ACHOSC_RS24065"
                     /old_locus_tag="pgaptmp_004813"
     CDS             1153..1608
                     /gene="cesD"
                     /locus_tag="ACHOSC_RS24065"
                     /old_locus_tag="pgaptmp_004813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SycD/LcrH family type III secretion system
                     chaperone CesD"
                     /protein_id="HGU4550584.1"
                     /translation="MSRKFSSLQDIYDFYQDGGTLASLTNLTQQDLNDLHSYAYTAYQ
                     SGDVITARNLFHLLTYLEHWNYDYTLSLGLCHQRLSNHEDAQLCFARCATLVMQDPRA
                     SYYSGISYLLVGNRKMAKKAFKACLMWCNEQEKYATYKENIKKLLGNTD"
     gene            complement(1994..>2176)
                     /locus_tag="ACHOSC_RS24070"
                     /old_locus_tag="pgaptmp_004814"
     CDS             complement(1994..>2176)
                     /locus_tag="ACHOSC_RS24070"
                     /old_locus_tag="pgaptmp_004814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CaiF/GrlA family transcriptional regulator"
                     /protein_id="HGU4550585.1"
                     /translation="YGNLHYKRLEIFIYDVNLEAVPIESPVSTGPKRKNYRVGNGIVG
                     QSNIWNEMIMRRKKEN"
BASE COUNT          708 a          425 c          416 g          627 t
ORIGIN      
        1 actcctgtaa cgtgagaaaa atcacaacat ttaactcgat tgaagttcgt ggcgttcctg
       61 aatgcataaa atacattaca tctttatctg aaagtcttga taaagaagca cagtctaaag
      121 caaaaaataa agatgtcgtt agagtattta aattaaacta tgcatcagcg acagatataa
      181 cgtataaata ccgcgaccaa aatgttgtcg tgcctggcgt cgtctccatc ctgaagacga
      241 tggcaagtaa tggctcactt cccagcacag gcaaaggtgc tgtagaaaga aacggcaacc
      301 tgtttgataa cagtgtaaag atttccgcag atccacgtct taatgcagtt gttgtaaaag
      361 atcgtgaaat aactatggac atatatcggc aattaatttc tgagcttgat attgagcaaa
      421 ggcaaattga gatttctgtt tcaattattg atgttgatgc aaatgatctt cagcaactgg
      481 gagtaaactg gtccggtaca ttaaatgcag ggcaaggttc tattgccttt aacagctcaa
      541 cggcgcaagc taatctcagt tcttctgtga taagcaacgc caataatttc atgatcagag
      601 tgaatgcgtt acaacagaac tcgaaagcaa aaatactctc tcagccatca attataacct
      661 taaataatat gcaggcgatt cttgataaaa acgtaacgtt ttatacaaaa gtttccggag
      721 aaaaggtggc atctctggaa agtattacca gcggaacatt actgagagta acaccacgta
      781 tacttgatga caactcgaat agtcttaccg gaaaacgtcg ggaacgagtt cgtttgttac
      841 ttgatattca ggatggcaat caatcgacta accaaagtaa tgctcaggat gccagcagca
      901 tcttacctga agtacaaaat tcagaaatga caacagaagc gacattaagc gctggagaaa
      961 gtctgctgct tggcggattt atccaggata aagagagttc aagtaaagat ggtataccgt
     1021 tgttaagtga tattcctgtt attggtaact tattctccag tacaacaaaa caaaaacaca
     1081 gcgttgttcg actctttttg attaaagcga ccccgataaa atcagcatct agcgaataat
     1141 aaatgaggct ttatgagcag gaaatttagc tctctacagg atatctatga tttctaccag
     1201 gatggtggta cgttagcatc actgacaaat ttgacgcaac aagatctcaa tgatcttcat
     1261 tcttatgcct atacagcata tcaatctggt gatgttataa ccgcaagaaa tctatttcat
     1321 ctgctaacat atctggagca ctggaattat gactacaccc tatctctggg gttgtgtcat
     1381 cagcgtttat caaatcatga agatgcacaa ctgtgtttcg ctcgctgtgc aactttagtt
     1441 atgcaagatc ccagggcatc ttattattct gggattagct acttattggt cggaaatcgg
     1501 aaaatggcca aaaaagcctt taaggcttgt ttaatgtggt gtaatgaaca agaaaaatac
     1561 gctacatata aagaaaatat taaaaaattg ttaggtaata cagattaaaa actaccatct
     1621 gccataccaa tccccaggct gtctggggat tttctatcgg aaataataaa gctgctttgg
     1681 tgaagtgcgc ccaacgttga gcaccgaccg ctggcagatt tatctacagg agtgggttac
     1741 tccattaacc aggattttat gcgcctgaac catcttctgc tgtactcaat gtcatttccg
     1801 cttatagaat aagcagtata tatcttaact tagcatcaga aatctgactg tttcaatcat
     1861 ctatagccca gagtgtattt gtcaggaaat gactttcaaa ctttggcaga gaaaaaggct
     1921 tatcctggaa aacaaagccc ttgaatagag ctttattttt attcttctat aaaatatact
     1981 caaaaaaata cgtttaattc tcctttttcc gcctcatgat catttcgttc cagatattag
     2041 actgccccac aataccatta ccgactcggt agttttttct ttttggtccg gttgataccg
     2101 gactttctat cggaacagcc tcgaggttaa catcataaat gaaaatctca aggcgcttat
     2161 aatgcaagtt accata
//
LOCUS       DAZKHF020000417         2175 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2201S31241
            SAMN43267800-rid25106413.denovo.419, whole genome shotgun sequence.
ACCESSION   DAZKHF020000417 DAZKHF020000000
VERSION     DAZKHF020000417.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267800
            Sequence Read Archive: SRR30307263
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2175)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2175)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 2175)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350581.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350581.2
            Genome Coverage       :: 151x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:21:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,652
            CDSs (total)                      :: 5,557
            Genes (coding)                    :: 5,320
            CDSs (with protein)               :: 5,320
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 237
            CDSs (without protein)            :: 237
            Pseudo Genes (ambiguous residues) :: 0 of 237
            Pseudo Genes (frameshifted)       :: 102 of 237
            Pseudo Genes (incomplete)         :: 130 of 237
            Pseudo Genes (internal stop)      :: 52 of 237
            Pseudo Genes (multiple problems)  :: 41 of 237
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2175
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267800-rid25106413.denovo.419"
                     /serotype="O 26:H11"
                     /isolate="2201S31241"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-01"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            81..329
                     /locus_tag="ACHOSC_RS24075"
                     /old_locus_tag="pgaptmp_004815"
     CDS             81..329
                     /locus_tag="ACHOSC_RS24075"
                     /old_locus_tag="pgaptmp_004815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excisionase"
                     /protein_id="HGU4550586.1"
                     /translation="MARLILLTEWAKEEFSEPVPTPSTLSKYAKAGMIFPLPKKVGRR
                     WRVDPQARFVGMVNKPEVIATDHPALKRILEDGAPAKI"
     gene            307..1437
                     /locus_tag="ACHOSC_RS24080"
                     /old_locus_tag="pgaptmp_004816"
     CDS             307..1437
                     /locus_tag="ACHOSC_RS24080"
                     /old_locus_tag="pgaptmp_004816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008907 - integrase activity [Evidence
                     IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage integrase Arm DNA-binding
                     domain-containing protein"
                     /protein_id="HGU4550587.1"
                     /translation="MARPRKYKTDVPGLSPYFDKRNNKVYWRYRHPITGKNHGLGSID
                     QKLAETIAAEANSRLARQQMEQMLSLQEKIISDTGGSSTVTIFLNNYRKIQQERYENG
                     EIKLNTLKQKAAPLRVFDERFGTRPLDAITVKDVVSVLEEYKARGHNRMGQIFRKVLI
                     DVFREAQQTGDVPPGFNPAESAKKPQVRISRQRLTFDEWMMIYNAAEKDGYFLQRGML
                     LALMTGQRLSDICKMQFSDIRDGYLHVEQQKTGTRIAIPLALRCDKLNLTLDDVVSSC
                     RDCVLSPWLLHHHHAKGTAKRGGMVKPATLTVAFKKARDSVDYNWRANGTPPSFHEQR
                     SLSERLFREQGVDTKILLGHSNQKMTDIYNDARGKEWKKLVI"
     gene            complement(1483..2121)
                     /gene="ompW"
                     /locus_tag="ACHOSC_RS24085"
                     /old_locus_tag="pgaptmp_004817"
     CDS             complement(1483..2121)
                     /gene="ompW"
                     /locus_tag="ACHOSC_RS24085"
                     /old_locus_tag="pgaptmp_004817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein OmpW"
                     /protein_id="HGU4550588.1"
                     /translation="MKKLTVAALAVTTLLSGSAFAHEAGEFFMRAGSATVRPTEGAGG
                     TLGSLGGFSVTNNTQLGLTFTYMATDNIGVELLAATPFRHKIGTRATGDIATVHHLPP
                     TLMAQWYFGDASSKFRPYVGAGINYTTFFDNGFNDHGKEAGLSDLSLKDSWGAAGQVG
                     VDYLINRDWLVNMSVWYMDIDTTAKYKSGVTTVKDSVRLDPWVFMFSAGYRF"
BASE COUNT          632 a          515 c          522 g          506 t
ORIGIN      
        1 tcaggctgat ttttaatgtt caaccccggt cgttgcccac cagctatagt ggcggcgacc
       61 atgattagcg aacgacgctc atggcaagac ttattctgct cactgagtgg gcaaaagagg
      121 aattcagtga accggtccca actccgagta cgttaagtaa atacgctaaa gccggaatga
      181 tatttcctct ccccaaaaaa gttggaagac gctggcgagt ggatccgcaa gctcgctttg
      241 tcggaatggt aaacaagccg gaggtgatcg ccacagatca ccctgctttg aagaggatac
      301 tggaagatgg cgcgcccgcg aaaatataaa accgatgttc cgggattatc tccgtatttt
      361 gacaaaagaa ataacaaagt ttactggcgt tacaggcatc ccataacagg caaaaatcac
      421 ggtctcggca gtattgacca gaaactggca gaaactattg cagcagaagc gaacagccgt
      481 cttgcccggc agcaaatgga acaaatgctc agtctgcagg agaaaattat tagtgatacc
      541 ggcggttcat caaccgttac catttttctg aataattaca gaaaaattca acaggaaaga
      601 tatgaaaacg gcgagatcaa actcaacacg ctgaaacaga aagcggcccc tctcagggta
      661 tttgatgaac gttttggcac cagaccgtta gatgccataa ccgtaaaaga tgtggtatca
      721 gtactggaag agtacaaggc cagaggacat aacagaatgg gacaaatttt caggaaagta
      781 ctgatcgatg ttttccggga agctcagcaa acgggcgatg tcccgccagg ctttaaccct
      841 gcagaatcgg caaaaaaacc gcaggtgcgg atatcaagac agcgactgac ttttgatgag
      901 tggatgatga tttataacgc agcggaaaag gatggttact ttttacagcg cggtatgctg
      961 ctggcactga tgacaggcca gcgcctttca gatatttgca aaatgcaatt ttcggatatc
     1021 cgggatggtt atcttcatgt cgaacagcaa aaaacaggaa cccggattgc catccctctg
     1081 gctctgcgtt gcgataaatt aaatctcacc ctggatgatg tggtgtcatc ctgccgcgat
     1141 tgcgttctta gtccgtggct attgcaccac catcacgcga aagggacagc taagcgcggc
     1201 gggatggtta agccagcaac attaaccgtt gcatttaaaa aagcccggga ttctgtggat
     1261 tacaactggc gtgctaatgg cacccctccc tctttccatg agcagagatc tttatcagag
     1321 cgattgttca gagagcaggg ggttgatacc aaaattttgc taggccattc gaatcaaaaa
     1381 atgaccgata tttacaacga cgcacgcggt aaggaatgga aaaaactggt catttgatga
     1441 ccagttttgc agaggggttt tgcagaggtt ttgcagagaa aattaaaaac gatatcctgc
     1501 ggagaacata aacacccacg gatccagtcg taccgagtct tttacggtag taacaccaga
     1561 tttgtactta gccgtggtat cgatatccat gtaccacact gacatgttaa ccaaccagtc
     1621 acggttaatc agataatcaa cccccacctg cccggcagct ccccaggaat ctttcagact
     1681 gagatcggaa agccccgcct ctttgccatg atcgttaaat ccattatcaa agaaggtggt
     1741 gtagttaata ccggccccaa cgtaaggacg gaatttgctg ctggcatcac caaaatacca
     1801 ctgcgccatc agtgttggcg gcagatggtg aacggttgca atatcgccgg tcgcccgggt
     1861 gccgatttta tggcggaacg gcgtcgctgc cagtaattcc acaccaatgt tgtcggtcgc
     1921 catataagta aacgtcaagc ccagttgagt gttattggtc acgctgaatc cacccagact
     1981 tcctaacgta ccaccagcac cttctgttgg acgtacggtt gcagaacctg cacgcataaa
     2041 aaattcgcct gcttcatgcg caaaggcact gccagaaaga agagttgtta ctgccaaagc
     2101 cgccactgtt aactttttca tatccgctcc gtcgttatgg ttataaaaac gtgacgaata
     2161 tacctacatt ggagt
//
LOCUS       DAZKHF020000418         2165 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2201S31241
            SAMN43267800-rid25106413.denovo.420, whole genome shotgun sequence.
ACCESSION   DAZKHF020000418 DAZKHF020000000
VERSION     DAZKHF020000418.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267800
            Sequence Read Archive: SRR30307263
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2165)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2165)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 2165)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350581.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350581.2
            Genome Coverage       :: 151x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:21:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,652
            CDSs (total)                      :: 5,557
            Genes (coding)                    :: 5,320
            CDSs (with protein)               :: 5,320
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 237
            CDSs (without protein)            :: 237
            Pseudo Genes (ambiguous residues) :: 0 of 237
            Pseudo Genes (frameshifted)       :: 102 of 237
            Pseudo Genes (incomplete)         :: 130 of 237
            Pseudo Genes (internal stop)      :: 52 of 237
            Pseudo Genes (multiple problems)  :: 41 of 237
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2165
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267800-rid25106413.denovo.420"
                     /serotype="O 26:H11"
                     /isolate="2201S31241"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-01"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            complement(79..1125)
                     /gene="aroH"
                     /locus_tag="ACHOSC_RS24090"
                     /old_locus_tag="pgaptmp_004818"
     CDS             complement(79..1125)
                     /gene="aroH"
                     /locus_tag="ACHOSC_RS24090"
                     /old_locus_tag="pgaptmp_004818"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase AroH"
                     /protein_id="HGU4550589.1"
                     /translation="MNRTDELRTARIESLVTPAELALRYPVTPGVATHVTDSRRRIEK
                     ILNGEDKRLLVIIGPCSIHDLTAAMEYATRLQSLRNQYQSRLEIVMRTYFEKPRTVVG
                     WKGLISDPDLNGSYRVNHGLEMARKLLLQVNELGVPTATEFLDMVTGQFIADLISWGA
                     IGARTTESQIHREMASALSCPVGFKNGTDGNSRIAVDAIRAARASHMFLSPDKNGQMT
                     IYQTSGNPYGHIIMRGGKKPNYHADDIAAACDTLHEFDLPEHLVVDFSHGNCQKQHRR
                     QLEVCEDICQQIRNGSTAIAGIMAESFLREGTQKIVGGQPLTYGQSITDPCLGWEDTE
                     RLVEKLASAVDTRF"
     gene            complement(1282..2115)
                     /gene="ppsR"
                     /locus_tag="ACHOSC_RS24095"
                     /old_locus_tag="pgaptmp_004819"
     CDS             complement(1282..2115)
                     /gene="ppsR"
                     /locus_tag="ACHOSC_RS24095"
                     /old_locus_tag="pgaptmp_004819"
                     /EC_number="2.7.11.33"
                     /EC_number="2.7.4.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="posphoenolpyruvate synthetase regulatory
                     kinase/phosphorylase PpsR"
                     /protein_id="HGU4550590.1"
                     /translation="MDNAVDRHVFYISDGTAITAEVLGHAVMSQFPVTISSITLPFVE
                     NESRARAVKDQIDAIYHQTGVRPLVFYSIVLPEIRAIILQSEGFCQDIVQALVAPLQQ
                     EMKLDPTPIAHRTHGLNPNNLNKYDARIAAIDYTLAHDDGISLRNLDQAQVILLGVSR
                     CGKTPTSLYLAMQFGIRAANYPFIADDMDNLVLPASLKPLQHKLFGLTIDPERLAAIR
                     EERRENSRYASLRQCRMEVAEVEALYRKNQIPWINSTNYSVEEIATKLLDIMGLSRRM
                     Y"
BASE COUNT          536 a          544 c          564 g          521 t
ORIGIN      
        1 gaccagagca agcagtaatg tgctgagaag acaacaagtt gcgcagaaat gcccatccca
       61 tcaggaatgg gcacgcattc agaagcgggt atctaccgca gaggcaagtt tttcgaccag
      121 gcgttcggta tcttcccagc ccagacacgg gtcggtaatg gattgaccgt aagtgagcgg
      181 ctgaccgccg acgatttttt gcgttccttc gcgcaggaaa ctttccgcca taattccagc
      241 aatcgccgta gagccattgc ggatttgctg acaaatatcc tcacaaactt ctaactggcg
      301 acggtgctgc ttctggcagt taccgtggct gaaatccacc accagatgtt caggtaaatc
      361 aaactcgtgc agcgtatcgc aggctgcggc gatatcatcg gcatgataat tcggtttttt
      421 gccgccacgc ataataatgt ggccatacgg gttgccgctg gtctgataga tggtcatctg
      481 accatttttg tctggcgaga gaaacatatg gctggcgcgg gctgcgcgga tagcatccac
      541 ggcaatccgc gagttgccat cggtaccatt tttaaaacct accggacagg agagtgccga
      601 agccatttca cggtggatct gactttcggt agtacgtgcg ccaatcgcgc cccaactgat
      661 taaatcagca ataaactgac cggtcaccat atcgaggaac tcagtcgcgg tagggacacc
      721 cagctcattt acctgtaaaa gtaatttgcg cgccatctcc agaccgtgat ttacccgata
      781 gctgccgttt aaatccggat cggagattag ccctttccag ccgacaacag ttcgtggttt
      841 ttcaaaatag gtgcgcatta cgatttccag ccgtgactgg tactggttgc gcagcgactg
      901 cagacgggtg gcgtactcca ttgcagcggt gagatcgtgg atcgagcagg ggccaataat
      961 gaccaacagt cgcttatctt caccattcag tattttttca attctgcggc gggagtcggt
     1021 gacatgggtg gcgacgccag gcgttacggg ataccgtagc gcgagttcgg cgggcgttac
     1081 caggctctca atacgcgcag tacggagttc gtcagttctg ttcattacaa gtctcagaaa
     1141 tttgttgctt ctacggcagg actaccggga agtgatgcgc atcacgataa accaatcgca
     1201 gctgatttca agtgcgaggt gtgtcacacc tcgccgggtg gttagcatga taacaaaaaa
     1261 ataagctaat gcactagttc tctagtacat tcggcggcta agccccatga tatcgaggag
     1321 cttggtggca atctcttcta ccgaataatt ggtactgtta atccacggga tctgattttt
     1381 acggtacaac gcttctactt ccgcgacttc catcctgcac tgacgaagcg aggcatagcg
     1441 gctgttctcc cgacgttcct cgcgaatcgc cgccagacgt tccgggtcga tagtcaggcc
     1501 gaacaattta tgctgaagcg gtttgagcga cgcgggtagc accagattgt ccatatcgtc
     1561 ggcaataaag gggtagtttg ccgcgcggat accaaactgc attgccagat acagactggt
     1621 gggggtttta ccacagcgag aaacaccgag caggatcacc tgagcctggt ccagattgcg
     1681 caacgaaatg ccgtcatcgt gggcgagggt gtaatcaatc gccgcaatgc gcgcatcata
     1741 tttattgaga ttattagggt taaggccatg ggtacgatga gcaatcggcg ttggatccag
     1801 tttcatctct tgttgtagcg gggcaaccag cgcctgaaca atatcctggc aaaagccttc
     1861 actttgcaag atgatggcgc gaatctccgg caacacgatg gagtagaaga ccagcgggcg
     1921 cacacctgtc tggtgataaa ttgcgtcaat ctgatccttc actgcccgtg cacggctctc
     1981 attttcgaca aacggcagcg taatgctgct gatagtgacg ggaaattgtg acattactgc
     2041 gtgtcctaat acctccgcag ttattgccgt accatcagaa atataaaaaa cgtggcgatc
     2101 aacagcatta tccattttgt tcttcccgtg atgcagacat aattctatga aagcataaat
     2161 taaaa
//
LOCUS       DAZKHF020000419         2153 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2201S31241
            SAMN43267800-rid25106413.denovo.421, whole genome shotgun sequence.
ACCESSION   DAZKHF020000419 DAZKHF020000000
VERSION     DAZKHF020000419.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267800
            Sequence Read Archive: SRR30307263
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2153)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2153)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 2153)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350581.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350581.2
            Genome Coverage       :: 151x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:21:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,652
            CDSs (total)                      :: 5,557
            Genes (coding)                    :: 5,320
            CDSs (with protein)               :: 5,320
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 237
            CDSs (without protein)            :: 237
            Pseudo Genes (ambiguous residues) :: 0 of 237
            Pseudo Genes (frameshifted)       :: 102 of 237
            Pseudo Genes (incomplete)         :: 130 of 237
            Pseudo Genes (internal stop)      :: 52 of 237
            Pseudo Genes (multiple problems)  :: 41 of 237
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2153
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267800-rid25106413.denovo.421"
                     /serotype="O 26:H11"
                     /isolate="2201S31241"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-01"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            154..2139
                     /gene="yjcS"
                     /locus_tag="ACHOSC_RS24100"
                     /old_locus_tag="pgaptmp_004820"
     CDS             154..2139
                     /gene="yjcS"
                     /locus_tag="ACHOSC_RS24100"
                     /old_locus_tag="pgaptmp_004820"
                     /EC_number="3.1.6.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418507.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkyl sulfatase YjcS"
                     /protein_id="HGU4550591.1"
                     /translation="MNNSRLFRLSRIVIALTAASGMMVNTANAKEEAKAATQYTQQVN
                     QNYAKSLPFSDRQDFDDAQRGFIAPLLDEGILRDANGKPYYRGEDYKFDINAPAPETV
                     NPSLWRQSQLNGISGLFKVTDRMYQVRGQDISNITFIEGDTGIIVIDPLVTPPSAKAA
                     LDLYFQNRPQKPIVAVIYTHSHTDHYGGVKGIISEADVKSGKVQVIAPAGFMDEAISE
                     NVLAGNIMSRRALYSYGLLLAHNPQGNIGNGLGVTLASGYPSIIAPNKTITKTGEKMI
                     IDGLEFDFLMTPGSEAPAEMHFYIPALKALCTAENATHTLHNFYTLRGAKTRDTSKWT
                     EYLNETLDMWGNDAEVLFMPHTWPVWGNKHINDYIGKYRDTIKYIHDQTLHLANQGYT
                     MNEIGDMIKLPPALANNWASRGYYGSVSHNARAVYNFYLGYYDGNPANLHPYGQVEMG
                     KRYVQALGGSARVINLAQEANKQGDYRWSAELLKQVIAANPGDQVAKNLQANNFEQLG
                     YQAESATWRGFYLTGAKELREGVHKFSHGTTGSPDTIRGMSVEMLFDFMSVRLDSAKA
                     AGKNISLNFNMSNGDNLNLTLNDSVLNYRKTLQSQADASFYISREDLHAVLTGQAKMA
                     DLVKAKKAKIIGNGAKLEEIIACLDNFDLWVNIVTPN"
BASE COUNT          574 a          570 c          525 g          484 t
ORIGIN      
        1 ccccttcagc acaaatgcct aataaaatgg atcgttccgc tcacacttcc acttattaaa
       61 gtcgcaaatt cccaattatg ataacgccag ctttaaatga cgtattttgc atggattaca
      121 tatttacgac acaaatgata aatggagatt tatatgaata actctcggtt attccgtttg
      181 agcaggattg ttattgcgtt aactgctgcc agcggcatga tggtaaatac cgctaacgcg
      241 aaagaggaag cgaaagccgc cactcaatat acccaacagg ttaatcagaa ttacgccaaa
      301 tcattaccgt ttagcgatcg tcaggatttt gacgacgctc agcgtggatt tatcgctccg
      361 ctgctggatg aaggtattct gcgcgatgct aatggcaaac catattatcg cggggaagat
      421 tataaatttg atatcaatgc gcccgcgccc gaaaccgtta accctagcct gtggcgtcag
      481 tcgcagttaa acggtatttc cggcctgttt aaagtcaccg acagaatgta ccaggttcgc
      541 ggtcaggata tctccaacat cacctttatc gaaggtgata ccgggattat tgtcatcgac
      601 ccgctggtga cgccaccgag cgcaaaagcc gcccttgacc tttacttcca aaatcgcccg
      661 caaaaaccga ttgttgcggt tatttatacc cacagccata ccgaccacta tggcggcgtg
      721 aaaggcatta tctctgaagc tgatgtgaaa tccggtaaag tgcaggttat cgcccctgct
      781 ggctttatgg acgaagccat cagcgaaaac gtactggcgg gtaatattat gagccgccgt
      841 gcactttact cctacggcct gctgctggcg cataaccctc agggtaacat cggcaatggt
      901 cttggcgtaa cgctggcatc gggctacccg agcatcatcg caccgaacaa aaccatcacc
      961 aaaaccggtg agaagatgat tatcgacggc ctggagtttg acttcctgat gaccccaggt
     1021 agcgaagcac cagccgaaat gcacttctat attccggccc tgaaagcgct gtgtaccgcc
     1081 gagaacgcca cgcataccct gcacaacttc tacactctac gcggcgcgaa aacccgcgac
     1141 accagcaagt ggaccgagta tctgaacgaa acgctggata tgtggggtaa cgacgcggaa
     1201 gtcctgttta tgccgcacac ctggccggtc tggggcaata agcatatcaa tgattatatt
     1261 ggtaaatatc gcgatactat caagtacatt cacgaccaga ccctgcacct ggcgaaccag
     1321 ggctacacca tgaatgaaat cggcgacatg attaaactgc cgcctgcact tgccaataac
     1381 tgggccagcc gtggctatta cggttctgtc agccacaacg cccgcgcggt gtataacttc
     1441 tatcttggct attacgacgg taacccggct aacctgcatc cgtatggtca ggtggagatg
     1501 ggtaaacgtt acgtgcaggc gctgggcggt tctgcccgtg tcatcaacct ggcgcaagaa
     1561 gcgaacaagc aaggtgatta ccgctggtcg gcagaactgc tgaaacaggt gattgccgcc
     1621 aacccgggtg accaggtcgc gaagaatctg caagcgaata actttgaaca gctgggctat
     1681 caggccgagt ccgccacctg gcgcggtttc tacctgaccg gcgcgaaaga gctgcgcgaa
     1741 ggggtgcata agttcagcca cggcaccacc ggttccccgg acaccattcg cgggatgtcg
     1801 gtcgaaatgc tgttcgactt tatgtccgtt cgcctcgata gcgcgaaagc cgcgggtaaa
     1861 aatatcagcc tgaacttcaa tatgagcaat ggcgataacc tcaacctgac gctgaacgat
     1921 agcgtgctta actaccgtaa aacactgcaa tcccaagctg acgcctcttt ctacatcagc
     1981 cgtgaagatc tgcacgccgt gctgaccggg caagccaaaa tggcggatct ggtaaaagcg
     2041 aagaaagcca aaattattgg caatggcgcg aaactggaag aaattatcgc ctgtctggat
     2101 aatttcgatt tgtgggtgaa tatcgtaacc ccaaattaat gtcactcgct ttt
//
LOCUS       DAZKHF020000420         2136 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2201S31241
            SAMN43267800-rid25106413.denovo.422, whole genome shotgun sequence.
ACCESSION   DAZKHF020000420 DAZKHF020000000
VERSION     DAZKHF020000420.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267800
            Sequence Read Archive: SRR30307263
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2136)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2136)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 2136)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350581.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350581.2
            Genome Coverage       :: 151x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:21:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,652
            CDSs (total)                      :: 5,557
            Genes (coding)                    :: 5,320
            CDSs (with protein)               :: 5,320
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 237
            CDSs (without protein)            :: 237
            Pseudo Genes (ambiguous residues) :: 0 of 237
            Pseudo Genes (frameshifted)       :: 102 of 237
            Pseudo Genes (incomplete)         :: 130 of 237
            Pseudo Genes (internal stop)      :: 52 of 237
            Pseudo Genes (multiple problems)  :: 41 of 237
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2136
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267800-rid25106413.denovo.422"
                     /serotype="O 26:H11"
                     /isolate="2201S31241"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-01"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            <1..2000
                     /locus_tag="ACHOSC_RS24105"
                     /old_locus_tag="pgaptmp_004821"
     CDS             <1..2000
                     /locus_tag="ACHOSC_RS24105"
                     /old_locus_tag="pgaptmp_004821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HGU4550592.1"
                     /translation="IVVYHQEQVNGPLVDESGRVIFADFNPSKRPWSVASDDSNNSWN
                     PAYNCFDRPGKKCISFTLRINGKDHDLLAVDKIHVDLNWRYLNEYLDQISANDEVLFL
                     KQGHEIIAKNQLAREKLIIYNSEGNYNIIDSVDTEYIAKTSAVPNNALFEIYFYYPGG
                     NLLNASDKLFYLPFAFIIIVLLVVYFMTTRVFRRQFSEMTELVNTLAFLPDSTDQIEA
                     LKIREGDAKEIISIKNSIAEMKDAEIERSNKLLSLISYDQESGFIKNMAIIESNNNQY
                     LAVGIIKLCGLEAVEAVFGVDERNKIVRKLCQRIAEKYAQCCDIVTFNADLYLLLCRE
                     NVQTFTRKIAMVNDFDSSFGYRNLRIHKSAICEPLQGENAWSYAEKLKLAISSIRDHM
                     FSEFIFCDDAKLNEIEENIWIARNIRHAMEIGELFLVYQPIVDINTRAILGAEALCRW
                     VSAERGIISPLKFITIAEDIGFINELGYQIIKTAMGEFRHFSQRASLKDDFLLHINVS
                     PWQLNEPHFHERFTTIMKENGLKANSLCVEITETVIERINEHFYLNIEQLRKQGVRIS
                     IDDFGTGLSNLKRFYEINPDSIKVDSQFTGDIFGTAGKIVRIIFDLARYNRIPVIAEG
                     VESEDVARELIKLGCVQAQGYLYQKPMPFSAWDKSGKLVKE"
     gene            2008..>2136
                     /locus_tag="ACHOSC_RS24110"
                     /old_locus_tag="pgaptmp_004822"
     CDS             2008..>2136
                     /locus_tag="ACHOSC_RS24110"
                     /old_locus_tag="pgaptmp_004822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4550593.1"
                     /translation="MSRINKFVLTVSLLIFIMISAVACGIYTQMVKERVYSLKQSVI"
BASE COUNT          611 a          409 c          500 g          616 t
ORIGIN      
        1 aaatcgttgt ttatcatcag gaacaagtaa atggaccgct tgtcgacgag tcagggcggg
       61 ttatttttgc cgattttaac ccatcgaaac gaccgtggtc ggtggcttca gatgactcta
      121 acaacagctg gaatccggca tacaattgct ttgatcgtcc gggtaaaaaa tgtatctctt
      181 ttacgctacg tatcaacggc aaagatcacg atttgttagc ggtagataaa attcatgtcg
      241 atttaaactg gcggtatctg aacgagtatc ttgatcaaat cagcgctaat gatgaagttc
      301 tatttttgaa acaaggtcat gagatcattg ccaagaatca actcgctcgt gaaaaactga
      361 ttatttataa tagcgaaggt aattataata ttattgattc tgtcgatacg gagtatattg
      421 caaagacatc agcagtgcca aataatgcat tgttcgaaat ctatttttat tatcctggcg
      481 gtaatttatt gaacgcatca gataaacttt tttatctacc gtttgcgttc attattatcg
      541 tgttgctggt ggtatatttc atgaccactc gtgtgttccg tcggcaattt tctgaaatga
      601 ccgagctggt taatacgctg gcgtttttgc ccgactcaac ggatcagatc gaggctctga
      661 aaattcgcga aggcgatgcg aaagagatta tcagcatcaa aaattcgatc gcggaaatga
      721 aagatgccga aattgaacgg tcaaataaat tgctctcact gatctcttac gatcaggaaa
      781 gcggttttat taaaaatatg gcgattattg agtccaacaa taatcagtat ctggctgtgg
      841 ggatcatcaa actgtgtggt ctggaagccg tggaagcggt gtttggtgtt gatgaacgca
      901 ataaaatcgt caggaaattg tgtcagcgaa ttgccgagaa atatgcgcaa tgctgcgata
      961 tcgtgacatt caatgccgat ctctatttac ttctgtgtcg ggaaaatgta cagacattta
     1021 cccgtaaaat agcgatggta aacgattttg acagcagctt tggctaccgc aatctgcgca
     1081 tccataagtc tgccatttgt gaacctttgc agggggaaaa cgcctggagt tacgcagaaa
     1141 aactgaaact ggcgatttcc agtatccgtg accatatgtt ctccgagttt attttctgtg
     1201 atgacgcgaa actcaacgaa atagaagaga atatctggat tgcgcgtaat attcgccatg
     1261 caatggaaat tggcgaacta ttcctcgtct atcaaccgat cgttgatatt aacacccgcg
     1321 ccattctggg cgcggaggcg ttgtgccgtt gggtttctgc ggagcggggg atcatttcac
     1381 cgctgaagtt cattaccatt gctgaagata tcgggtttat caatgagctg ggttatcaga
     1441 ttattaaaac ggcaatgggt gaattcagac attttagtca gcgtgcgtcg ctgaaggatg
     1501 atttcttact gcatattaat gtttcgccct ggcagttaaa cgaaccgcac tttcatgagc
     1561 gttttaccac catcatgaaa gaaaatggcc tgaaggcgaa cagcctctgt gttgagatca
     1621 ctgaaaccgt gatcgagcga attaatgaac atttttatct caatattgaa caactgcgta
     1681 aacaaggggt acggatatcg attgatgact ttggcaccgg tttgtcaaac ctgaaacgtt
     1741 tttatgaaat taatccagac agcataaagg tggactcgca atttaccggc gatattttcg
     1801 gtactgcggg aaaaattgtg cgcattattt tcgatctggc acgctataac cggatcccgg
     1861 tgattgcgga aggcgtagag agcgaagacg ttgcgcgcga attaattaaa ttaggatgtg
     1921 ttcaggctca ggggtatctg taccagaaac ccatgccatt ctccgcctgg gataaaagtg
     1981 gaaaattagt aaaagagtag tttacgtatg tccagaatca ataagttcgt acttacagtc
     2041 agtctgctga tttttatcat gatttcagca gttgcctgcg ggatctacac tcaaatggta
     2101 aaggaacggg tgtatagcct gaaacagtcc gttatt
//
LOCUS       DAZKHF020000421         2115 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2201S31241
            SAMN43267800-rid25106413.denovo.423, whole genome shotgun sequence.
ACCESSION   DAZKHF020000421 DAZKHF020000000
VERSION     DAZKHF020000421.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267800
            Sequence Read Archive: SRR30307263
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2115)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2115)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 2115)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350581.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350581.2
            Genome Coverage       :: 151x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:21:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,652
            CDSs (total)                      :: 5,557
            Genes (coding)                    :: 5,320
            CDSs (with protein)               :: 5,320
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 237
            CDSs (without protein)            :: 237
            Pseudo Genes (ambiguous residues) :: 0 of 237
            Pseudo Genes (frameshifted)       :: 102 of 237
            Pseudo Genes (incomplete)         :: 130 of 237
            Pseudo Genes (internal stop)      :: 52 of 237
            Pseudo Genes (multiple problems)  :: 41 of 237
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2115
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267800-rid25106413.denovo.423"
                     /serotype="O 26:H11"
                     /isolate="2201S31241"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-01"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            778..1755
                     /locus_tag="ACHOSC_RS24115"
                     /old_locus_tag="pgaptmp_004823"
     CDS             778..1755
                     /locus_tag="ACHOSC_RS24115"
                     /old_locus_tag="pgaptmp_004823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052630.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RepB family plasmid replication initiator
                     protein"
                     /protein_id="HGU4550594.1"
                     /translation="MDKSSGELVTLTPNNNNTVQPVALMRLGVFVPTLKSLKNSKKNT
                     LSRTDATEELTRLSLARAEGFDKVEITGPRLDMDNDFKTWVGIIHSFARHNVIGDKVE
                     LPFVEFAKLCGIPSSQSSRRLRERISPSLKRIAGTVISFSRTDEKHTREYITHLVQSA
                     YYDTERDIVQLQADPRLFELYQFDRKVLLQLKAINALKRRESAQALYTFIESLPRDPA
                     PISLARLRARLNLKSPVFSQNQTVRRAMEQLREIGYLDYTEIQRGRTKFFCIHYRRPR
                     LKAPNDESKENPLPPSPAEKVSPEMAEKLALLEKLGITLDDLEKLFKSR"
     gene            1939..>2115
                     /locus_tag="ACHOSC_RS24120"
                     /old_locus_tag="pgaptmp_004824"
     CDS             1939..>2115
                     /locus_tag="ACHOSC_RS24120"
                     /old_locus_tag="pgaptmp_004824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010904653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HGU4550595.1"
                     /translation="MQKNVTPGRRKGCPNYPPEFKQQLVAASCEPGISISKLALENGI
                     NANLLFKWRQQWREG"
BASE COUNT          569 a          520 c          504 g          522 t
ORIGIN      
        1 gtctccagga ttcccggggc ggttcagtgg ataaatgctt attacaacgt ttatcgacct
       61 catacaaaca atggtggttt atcgccttgc cgcatgaaga aaaatggaaa caggttattc
      121 cggtgtcctg attttatgat ccactacaca ttcctgacga acgccactgc ataaatcaca
      181 ttgcacatcc tcagagagta cgcagtatag gaggggtata tatacattat cggtatatca
      241 ggcgacttct gtctgtatgt tgtcggtgct cgccgcagac cggtgaccgg gcgaagcccg
      301 cgaaccggcg aggaagcgca atgtatctgt agttacagtg agcagatacg ccctgaagct
      361 tcctcataaa acagttgtgt aaactgtttt cccggaatga tttatccaca tatccggcgg
      421 cccgatccag taaacagatc cataaatgat caagaaatga tccattaaag atccccatat
      481 cgctgcaaac cttgtcactc atgctccggg accacgatca cataagctgt ggcatgtttc
      541 tgataaactg tagcaggatc atgataagct gtattcagta atgtatatac tgtagtaagt
      601 taatgacata aattatggtc agtacgccag gctcagctgt taaatacaga ctgcaggttt
      661 ttcttcagtc agttgacggg gttctgacac tcaattttct gtttattctt ttactgtcca
      721 caggcaggag gctttctgga aaacgaaaat tcagacatca aaaaactgtt cggcgaggtg
      781 gataagtcgt ccggtgagct ggtgacactg acaccaaaca ataacaacac cgtacaacct
      841 gtggcgctga tgcgtctggg cgtttttgta ccgaccctta aatcactgaa gaacagtaaa
      901 aaaaatacac tgtcacgtac tgatgccacg gaagagctga cacgtctttc cctggcccgt
      961 gctgagggat ttgataaggt tgagatcacc ggcccccgcc tggatatgga taacgatttc
     1021 aagacctggg tgggaatcat tcattccttt gcccgccata acgtaattgg tgacaaagtt
     1081 gaactgcctt ttgtcgagtt tgcaaaactg tgtggtatac cttcaagcca gtcatcccgc
     1141 aggctgcgtg agcgcatcag cccttccctg aaacgcattg ccggtaccgt gatctccttt
     1201 tcccgcaccg atgagaagca cacccgggaa tatatcaccc atctggtaca gtcagcctac
     1261 tacgatactg aacgggatat tgttcagtta caggctgatc cccgcctttt tgaactgtac
     1321 cagtttgacc ggaaagtgct gctgcagctc aaggccatca atgccctgaa gcgacgggag
     1381 tccgcccagg cactttacac ctttatagag agcctgcccc gggatccggc accgatatcg
     1441 ctggcgcggc tgcgtgcccg cctcaatctg aagtctcctg tattttccca gaaccagacg
     1501 gtcagacggg caatggagca gttgcgcgag attggatatc ttgattacac ggagatccag
     1561 cgggggcgga caaaattctt ctgtattcac taccggcgtc cccggttaaa agcgccgaat
     1621 gatgagagta aggaaaatcc gttgccacct tcacctgcgg aaaaagtcag tccggagatg
     1681 gcggagaaac ttgccctgct tgaaaaactg ggcatcacgc tggatgacct ggaaaaactc
     1741 ttcaaatccc gctgaacata aactgtagtc agtgaagagt gctcctttac tgactacagc
     1801 ttatattatc aggtgcagtg agtggtctgc tcactgcagt ttatatgtaa gcgtacagcc
     1861 tgaaccgtct ggtcagaatc tgacgaatta gacaaagtgg tgtccaccaa ataagtagtg
     1921 ggaaccaaag tgtcagatat gcagaaaaat gtgactcccg gcaggcgaaa aggctgccct
     1981 aattatcctc ccgaatttaa acagcagctc gttgctgcct cctgtgaacc cgggatatcc
     2041 atctcaaaac ttgctcttga aaatggcatt aacgccaatc tgttgttcaa atggcgacaa
     2101 caatggcgcg aggga
//
LOCUS       DAZKHF020000422         2095 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2201S31241
            SAMN43267800-rid25106413.denovo.424, whole genome shotgun sequence.
ACCESSION   DAZKHF020000422 DAZKHF020000000
VERSION     DAZKHF020000422.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267800
            Sequence Read Archive: SRR30307263
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2095)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2095)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 2095)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350581.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350581.2
            Genome Coverage       :: 151x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:21:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,652
            CDSs (total)                      :: 5,557
            Genes (coding)                    :: 5,320
            CDSs (with protein)               :: 5,320
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 237
            CDSs (without protein)            :: 237
            Pseudo Genes (ambiguous residues) :: 0 of 237
            Pseudo Genes (frameshifted)       :: 102 of 237
            Pseudo Genes (incomplete)         :: 130 of 237
            Pseudo Genes (internal stop)      :: 52 of 237
            Pseudo Genes (multiple problems)  :: 41 of 237
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2095
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267800-rid25106413.denovo.424"
                     /serotype="O 26:H11"
                     /isolate="2201S31241"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-01"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            16..1671
                     /locus_tag="ACHOSC_RS24125"
                     /old_locus_tag="pgaptmp_004825"
                     /pseudo
     CDS             16..1671
                     /locus_tag="ACHOSC_RS24125"
                     /old_locus_tag="pgaptmp_004825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310188.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1983 domain-containing protein"
     gene            1739..>2095
                     /locus_tag="ACHOSC_RS24130"
                     /old_locus_tag="pgaptmp_004826"
     CDS             1739..>2095
                     /locus_tag="ACHOSC_RS24130"
                     /old_locus_tag="pgaptmp_004826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044384 - host outer membrane [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Ail/Lom family outer membrane beta-barrel
                     protein"
                     /protein_id="HGU4550596.1"
                     /translation="MRKLYAAILSAAICLAVSGAPAWASEHQSTLSAGYLHVSTNVPG
                     SDELNGINVKYRYEFTDTLGMVTSFSYAGDRNRQLTHYSDTRWHEDFVRNRWFSVMAG
                     PSVRVNEWFSAYAMAGV"
BASE COUNT          520 a          522 c          613 g          440 t
ORIGIN      
        1 gccagctttg agccgcagtc aggcaccctg aacagcgtta tccctccggc agtgcagcac
       61 ctgacggtgg aggtgagcgc ggctgacggt cagtatctgg cacaggcgaa atgggacacg
      121 ccgcgggtgg tgaagggcgt gcgcttcagt ctgcgcctga ccagcggaag cggtgagaac
      181 agccgcctgc tgaccaccgc catcactgcc gatacggagc accgtttcag tggcctgcct
      241 ctcggggaat acaccctgac ggtcagggcg attaacagct atggccagca gggcgaaccc
      301 gccaccacca cgttccggat taacgcaccg gcagcgcctg ccggtgttga actgacgccg
      361 gggtattttc agataacggc agtcccgaaa ctgaccattt atgacccgac ggtgcagttt
      421 gagttctggt tttcggaggc aaaaattgca gacgccgcac aggtggaaac ctctgcccgt
      481 tatctgggga ccggcagtca gtggagtgtc tccggtccgc acattaagcc cggaaaggat
      541 ttctggtttt atgtgcgcag cgtcaacctg gtggggaaat ctgcttttgt ggaagccagt
      601 ggccgggcca gcaatgatgc agaagggtat ctggggctgt ttcgggaaaa aataggaaaa
      661 ctgcatctgg ctcaggggct gtgggagctg atagacaaca gccagcttgc ggatgagatg
      721 gcggagatga agaccagcat caccgaaacc cgcaatgaaa tcacacagac ggtcagtaaa
      781 acactggaga accagagcgc caccatacag cagatacagc gcgtgcagaa ggacacaaat
      841 gatgacctgg ctgcgctgta catgctgaag gttcaaaaaa cgaaagacgg cattccctat
      901 gtggccggga ttggtgcagg gattgaggat actgatggcc agccactgag caacatactg
      961 ctgctggctg accgtatcgc gatgataaat ccggagagcg gcaacagcac gccgttattt
     1021 gtggcgcagg ggaatcagtt gttcatgaac gatgtgttcc tgaagcggct gtttgcggcg
     1081 agtatcacgt catccggcaa tcctccggca ttttccctga cgccggacgg gcgactgacg
     1141 gcgaaaaatg cggatatcag tggcagtgtg aatgcgaact cagggacgct caacaacgtc
     1201 acgattaatg agaactgtca gattaagggg aaactgtcag ccaaccagat tgaaggcgat
     1261 attgtcaaaa cggtcagcaa gtctttcccc cgcacgagca cttatgccag tggcaccatc
     1321 acggtaagaa tcagtgatga tcagaagttt gaccggcagg tcatgatacc gccagtgtta
     1381 ttccgcggtg gtaagcatga gaatttcaac agtaataacc aacagtcata ctggtattca
     1441 acctgccggt taagagtgac ccgcaatggt caggagattt ttaatcagtc cacgacggat
     1501 gctcagggcg tattttcctc agttatagat atgcctgccg gacaggggac gctgacactg
     1561 acattcaccg tatcttcatc aggagcgaat aactggacac caacaaccag tatcagcgat
     1621 ctgttggttg ttgtgatgaa gaaagccacc gcaggcatca gtatcagctg aattttataa
     1681 cccatatacg ggcgccagaa atggcgcctt ttttattgca gaaaagcgag aggtaattat
     1741 gcgtaaactt tatgccgcca ttttgtccgc agccatttgt ctggccgtat ccggtgcgcc
     1801 tgcatgggca tctgaacatc agtccacgct gagcgcgggg tatcttcatg tctcgacgaa
     1861 cgttcctggc agcgatgaac tgaacgggat taacgtgaaa taccgttatg agtttacgga
     1921 cacactgggg atggtgacgt cgttcagcta tgcaggagac aggaatcgcc agctgaccca
     1981 ttacagcgat acccgctggc atgaagattt cgttcgtaac cgctggttca gcgtaatggc
     2041 ggggccgtct gtgcgcgtga atgaatggtt cagcgcgtat gcgatggcgg gcgtg
//
LOCUS       DAZKHF020000423         2094 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2201S31241
            SAMN43267800-rid25106413.denovo.425, whole genome shotgun sequence.
ACCESSION   DAZKHF020000423 DAZKHF020000000
VERSION     DAZKHF020000423.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267800
            Sequence Read Archive: SRR30307263
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2094)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2094)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 2094)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350581.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350581.2
            Genome Coverage       :: 151x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:21:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,652
            CDSs (total)                      :: 5,557
            Genes (coding)                    :: 5,320
            CDSs (with protein)               :: 5,320
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 237
            CDSs (without protein)            :: 237
            Pseudo Genes (ambiguous residues) :: 0 of 237
            Pseudo Genes (frameshifted)       :: 102 of 237
            Pseudo Genes (incomplete)         :: 130 of 237
            Pseudo Genes (internal stop)      :: 52 of 237
            Pseudo Genes (multiple problems)  :: 41 of 237
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2094
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267800-rid25106413.denovo.425"
                     /serotype="O 26:H11"
                     /isolate="2201S31241"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-01"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            complement(<1..401)
                     /locus_tag="ACHOSC_RS24135"
                     /old_locus_tag="pgaptmp_004827"
     CDS             complement(<1..401)
                     /locus_tag="ACHOSC_RS24135"
                     /old_locus_tag="pgaptmp_004827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001516617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase WbuB"
                     /protein_id="HGU4550597.1"
                     /translation="MKLALIIDDYLPHSTRVGAKMFHELGLELLSRGHDVTVITPDNT
                     LQAIYSVSMTDGIKVWRFKSGPLKDIGKAKRAINETLLSFRAWHALKHLIQHDTFDGI
                     VYYSPSIFWGGLVKKIKERCQCPSYLVLRDM"
     gene            complement(401..1531)
                     /gene="wecB"
                     /locus_tag="ACHOSC_RS24140"
                     /old_locus_tag="pgaptmp_004828"
     CDS             complement(401..1531)
                     /gene="wecB"
                     /locus_tag="ACHOSC_RS24140"
                     /old_locus_tag="pgaptmp_004828"
                     /EC_number="5.1.3.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000734424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008761 - UDP-N-acetylglucosamine
                     2-epimerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="non-hydrolyzing UDP-N-acetylglucosamine
                     2-epimerase"
                     /protein_id="HGU4550598.1"
                     /translation="MKKLKVMSVVGTRPEIIRLSRVLAKLDEYCDHLIVHTGQNYDYE
                     LNEVFFKDLGVRKPDYFLNAAGKNAAETIGQVIIKVDEVFEQEKPEAMLVLGDTNSCI
                     SAIPAKRRRIPIFHMEAGNRCFDQRVPEETNRKIVDHTADINMTYSDIAREYLLAEGV
                     PADRIIKTGSPMFEVLTHYMSQIDGSDVLSRLNLTPGNFFVVSAHREENVDTPKQLAK
                     LANILNTVAEKYDIPVVVSTHPRTRNRINENGIQFHKNILLLKPLGFHDYNHLQKNAR
                     AVLSDSGTITEESSIMNFPALNIREAHERPEGFEEGAVMMVGLESARVLQALEIIATQ
                     PRGEVRLLRQVSDYSMPNVSDKVVRIIHSYTDYVKRVVWKQY"
     gene            complement(1528..>2094)
                     /locus_tag="ACHOSC_RS24145"
                     /old_locus_tag="pgaptmp_004829"
     CDS             complement(1528..>2094)
                     /locus_tag="ACHOSC_RS24145"
                     /old_locus_tag="pgaptmp_004829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001529290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WxcM-like domain-containing protein"
                     /protein_id="HGU4550599.1"
                     /translation="LKLLEGANETGYRTFGPIYSVTVGEVAQLIYRFKESRQTLIIED
                     VGNGFTRALYSTWLSYLSPKQFAYTVPSYSDDRGVFCEVLKTKNAGQFSFFTAHPGIT
                     RGGHYHHSKNEKFIIIRGSACFKFENIVTGERYELNVSSDDFKIVETVPGWTHDITNN
                     GSDELVVMLWANEIFNRSEPDTIARVLS"
BASE COUNT          634 a          455 c          377 g          628 t
ORIGIN      
        1 aacatatccc ttaggaccag atagcttggg cactggcatc gctctttaat ttttttaacc
       61 aagcctcccc aaaaaataga gggggaataa taaacgatac catcaaatgt atcatgttgg
      121 atgagatgct ttaatgcgtg ccatgcacga aaagataaaa gagtttcatt tatggcacgt
      181 ttagccttac ctatatcctt taaaggtcca cttttgaaac gccaaacctt tataccatca
      241 gtcatgctaa cagaatagat tgcctgtaat gtgttgtcag gcgtaattac agttacatca
      301 tggcctctgc tcagcaattc caggcctaac tcatgaaaca ttttagcccc aactcgtgtg
      361 ctatggggca aataatcatc aatgattaat gcaagtttca ttagtattgc ttccagacaa
      421 cccgtttaac gtagtcagta tatgaatgga taatacgcac aactttatct gaaacatttg
      481 gcatactata gtcactgacc tgacgaagta agcgtacttc tccacgaggc tgtgttgcaa
      541 taatttctaa tgcctgtaac acacgggcag actcaagacc aaccatcatt acagcccctt
      601 cttcgaagcc ttcagggcgt tcgtgcgctt ctcgtatatt gagcgcaggg aagttcataa
      661 tggaggactc ctctgtaata gtcccgctat ccgataaaac agcacgtgca tttttttgca
      721 gatggttgta atcgtgaaat cctaatggct taagtagcaa gatattttta tgaaattgaa
      781 taccgttttc gttgatgcgg ttacgagtgc gcggatgagt agaaacaact accgggatat
      841 catatttttc agccacggta ttaagtatat ttgccagttt cgcaagctgt ttaggggtat
      901 caacattttc ttctctgtgg gcacttacca caaagaaatt cccaggtgtt aaattcaggc
      961 gagaaagtac atcggaacca tcaatctgcg acatataatg agtgagtact tcaaacattg
     1021 ggctaccggt tttaataatc ctatcggctg gtacaccttc agccaaaaga tattcacgcg
     1081 cgatatcact atatgtcata ttgatatcag cggtgtggtc aactattttt ctgttagttt
     1141 cttccggtac gcgttgatca aaacaacgat tcccagcctc catatggaaa atcggaattc
     1201 ttcgacgctt tgctggtatt gctgaaatac aggagttagt atcgccaaga actaacatgg
     1261 cttctggttt ttcctgttca aagacctcat caactttaat gataacttgt ccaatagtct
     1321 ctgctgcatt tttacctgcg gcattaagaa aataatcagg tttgcgaaca cccaaatcct
     1381 tgaaaaaaac ctcattcaat tcataatcgt aattttgccc agtatgaaca ataaggtgat
     1441 cacaatattc atctaatttt gcaaggacac gcgagagtcg aataatttct ggacgagtcc
     1501 caacaaccga catgactttc aattttttca cgataaaact ctcgctatag tatctggttc
     1561 agaacgatta aatatttcat ttgcccaaag cataacaact agctcatccg agccattatt
     1621 agtaatgtca tgcgtccatc ccggaactgt ttcaacaatt ttaaaatcat ctgatgaaac
     1681 attaagttca tatcgttcac ccgtgacaat attttcaaat ttgaaacaag cacttcctcg
     1741 gatgataata aatttctcat ttttggaatg atgataatgc cccccccgag taattcctgg
     1801 atgcgcagta aaaaacgaaa actggcccgc gtttttcgtt ttcaatactt cacagaatac
     1861 ccctctgtca tcactataag aaggaaccgt atacgcaaac tgtttaggag aaaggtaact
     1921 taaccatgtt gagtacaatg ctcgagtgaa tccattacct acatcttcga tgattaatgt
     1981 ttggcgactt tctttaaatc gataaattaa ttgtgccacc tcaccaacag taacagaata
     2041 aattggacca aatgtcctgt aaccagtttc gttcgctcct tctaatagct ttaa
//
LOCUS       DAZKHF020000424         2087 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2201S31241
            SAMN43267800-rid25106413.denovo.426, whole genome shotgun sequence.
ACCESSION   DAZKHF020000424 DAZKHF020000000
VERSION     DAZKHF020000424.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267800
            Sequence Read Archive: SRR30307263
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2087)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2087)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 2087)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350581.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350581.2
            Genome Coverage       :: 151x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:21:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,652
            CDSs (total)                      :: 5,557
            Genes (coding)                    :: 5,320
            CDSs (with protein)               :: 5,320
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 237
            CDSs (without protein)            :: 237
            Pseudo Genes (ambiguous residues) :: 0 of 237
            Pseudo Genes (frameshifted)       :: 102 of 237
            Pseudo Genes (incomplete)         :: 130 of 237
            Pseudo Genes (internal stop)      :: 52 of 237
            Pseudo Genes (multiple problems)  :: 41 of 237
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2087
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267800-rid25106413.denovo.426"
                     /serotype="O 26:H11"
                     /isolate="2201S31241"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-01"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            <1..1737
                     /locus_tag="ACHOSC_RS24150"
                     /old_locus_tag="pgaptmp_004830"
     CDS             <1..1737
                     /locus_tag="ACHOSC_RS24150"
                     /old_locus_tag="pgaptmp_004830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016032 - viral process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tape measure protein"
                     /protein_id="HGU4550600.1"
                     /translation="EQTRRLKENMGTLETWADKTGKAFKSMWDAILDIGRPESSADML
                     ASAQKAFDEADKKWQWYQSRSQRRGKTSSFRANLQGAWNDRENARLGLAAATLQSDME
                     KAGELAARDRAERDASQLKYTGEAQKAYERLLTPLEKYTARQEELNKALKDGKILQAD
                     YNTLMAAAKKDYESTLKKPKSSGVKVSAGERQEDQAHAALLALETELRTLEKHSGANG
                     KISQQRRDLWKAENQYAVLKEAATKRQLSEQEKSLLTHEKETLEYKRQLAELGDKVEH
                     QKRLNELAQQAARFEQQQGAKQAAISAQARGLTDRQVQRESEEQRLRDVYGDNPDALA
                     KATSALKNTWSAEEQLRGSWMAGLKSGWGEWAESATDSFSQVKSVATQTFDGIAQNMA
                     AMLTGSEQSWRGFTRSVLSMLTEIFLKQAMVGIVGSIGSAMGGAFGGGASASTGTAIQ
                     AAAANFHFATGGFTGTGGKYEPAGIVHRGEFVFTKEATSRIGVGNLYRLMRGYAEGGY
                     VGGAGSPAQMRRAEGISFNQNNHVVIQNDGTNGQAGPQLMKAVYDMARKGAQDEIQAQ
                     MRDGGVFSGGRR"
     gene            1734..2063
                     /locus_tag="ACHOSC_RS24155"
                     /old_locus_tag="pgaptmp_004831"
     CDS             1734..2063
                     /locus_tag="ACHOSC_RS24155"
                     /old_locus_tag="pgaptmp_004831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HGU4550601.1"
                     /translation="MKTFRWKVKPDMEVNSQPSVREVRFGDGYSQRMAAGLNADLKTY
                     RVTLSVTREEARHLEAFLAEHGGWKAFLWTPPYAWRQIKVTCAAWSSRVRMLRVEFSA
                     EFKQVVN"
BASE COUNT          498 a          496 c          723 g          370 t
ORIGIN      
        1 gagcagaccc gtcgcctgaa agaaaacatg ggaacactgg agacctgggc ggataaaaca
       61 gggaaggcat tcaaatcgat gtgggatgcc attctggata tcggtcgtcc ggaatcctca
      121 gcggatatgc tcgccagtgc gcagaaggca tttgatgagg cggataaaaa atggcagtgg
      181 taccagagtc ggagccagcg ccgcggtaaa acctcctctt tccgggccaa ccttcagggc
      241 gcatggaatg accgggaaaa tgcccgtctg gggctggcag cggccacgct gcagtcggat
      301 atggaaaaag ccggtgaact ggccgccagg gaccgggccg aacgggacgc atcacagctg
      361 aagtataccg gagaggcgca gaaggcgtat gagcgtctgc tgacgccgct ggagaaatat
      421 accgcccgtc aggaagaact gaataaggcc ctgaaagacg ggaaaatcct gcaggcggat
      481 tacaacacgc tgatggcggc ggcgaaaaag gattatgaat cgacgctgaa aaagccgaag
      541 tcgtcaggag tcaaagtgtc agccggtgag cgtcaggaag accaggcgca tgctgccctg
      601 ctggcgcttg aaaccgagct ccggacgctg gaaaaacaca gcggtgcgaa tgggaaaatc
      661 agccagcagc gtcgcgattt atggaaagcg gaaaatcagt atgcggtcct gaaagaggca
      721 gccacgaaac ggcagttatc tgagcaggaa aaatccctgc tgacccatga gaaagagacg
      781 ctggagtaca aacgccagct ggctgagctg ggagacaaag ttgaacacca gaaacggctg
      841 aatgagctgg cacagcaggc tgcgcggttt gagcagcagc agggcgcgaa gcaggcggca
      901 atcagtgccc aggcgcgggg cctcaccgac cgtcaggtgc agcgggagtc ggaagagcag
      961 cgccttcgtg acgtgtacgg tgataatccg gatgcgctgg cgaaggccac atctgcactg
     1021 aagaacacct ggtctgcgga ggagcagctt cgtggaagct ggatggccgg tctgaagtcc
     1081 ggctggggcg agtgggcaga aagtgcgacg gacagttttt cgcaggttaa aagcgtggcc
     1141 acgcagacct ttgacggtat tgcacagaat atggcagcga tgctgaccgg cagcgaacag
     1201 agctggcgtg gtttcacccg ttctgtgctc tccatgctga cagagatttt tctgaagcag
     1261 gccatggtgg ggattgtcgg gagtattggc agcgccatgg gtggtgcttt cggtggtggg
     1321 gcgtctgcct ccacggggac ggccattcag gctgcggcgg cgaacttcca tttcgcgacc
     1381 ggaggattta cgggaaccgg tggcaaatac gaacctgccg gtattgtcca ccgcggggag
     1441 tttgtcttca cgaaggaggc aaccagccgg attggcgtcg gcaacctgta tcgtctgatg
     1501 cgcgggtatg cggaaggtgg ttatgtgggc ggtgccggaa gtccggcgca gatgcggcgg
     1561 gcggaaggca ttagttttaa tcagaacaat cacgtggtga ttcagaacga cggcaccaac
     1621 ggacaggcgg ggccgcagct gatgaaggcg gtgtatgaca tggcccgcaa gggggcgcag
     1681 gatgagattc aggcgcagat gcgtgatggc ggcgtctttt ccggaggcag gcgatgaaaa
     1741 catttcgctg gaaagtgaag ccggatatgg aggtgaactc gcagccatcg gtgcgtgaag
     1801 tgcgttttgg tgacgggtat tcgcagcgta tggcggcggg gctgaatgct gacctgaaaa
     1861 cataccgtgt gacgctttcc gtgacccggg aggaggcccg acatctggag gcattcctgg
     1921 cagagcacgg tggctggaag gcgtttctgt ggacaccgcc ttatgcctgg cggcagataa
     1981 aggtgacctg tgccgcctgg tcatcacggg ttcgcatgct gcgggttgaa ttcagtgccg
     2041 agtttaagca ggtggtgaac tgatgcagga tattcacgaa gaaagtc
//
LOCUS       DAZKHF020000425         2085 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2201S31241
            SAMN43267800-rid25106413.denovo.427, whole genome shotgun sequence.
ACCESSION   DAZKHF020000425 DAZKHF020000000
VERSION     DAZKHF020000425.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267800
            Sequence Read Archive: SRR30307263
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2085)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2085)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 2085)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350581.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350581.2
            Genome Coverage       :: 151x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:21:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,652
            CDSs (total)                      :: 5,557
            Genes (coding)                    :: 5,320
            CDSs (with protein)               :: 5,320
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 237
            CDSs (without protein)            :: 237
            Pseudo Genes (ambiguous residues) :: 0 of 237
            Pseudo Genes (frameshifted)       :: 102 of 237
            Pseudo Genes (incomplete)         :: 130 of 237
            Pseudo Genes (internal stop)      :: 52 of 237
            Pseudo Genes (multiple problems)  :: 41 of 237
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2085
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267800-rid25106413.denovo.427"
                     /serotype="O 26:H11"
                     /isolate="2201S31241"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-01"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            <1..480
                     /locus_tag="ACHOSC_RS24160"
                     /old_locus_tag="pgaptmp_004832"
     CDS             <1..480
                     /locus_tag="ACHOSC_RS24160"
                     /old_locus_tag="pgaptmp_004832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PerC family transcriptional regulator"
                     /protein_id="HGU4550602.1"
                     /translation="LNDVQKFIEAHPGCTSGDIADAFAGYSRQRVLQSASKLRQSGRV
                     AHRCEGDTRRHFPRLTERAQEPEPQPVRETRPVRNFYVGTNDPRVILCLTRQAEELES
                     RGLYRRAATVWMAAFRESHSQPERNNFLARREQCLRKSSKRAVSGDEWYLSGNYVGA"
     gene            480..806
                     /locus_tag="ACHOSC_RS24165"
                     /old_locus_tag="pgaptmp_004833"
     CDS             480..806
                     /locus_tag="ACHOSC_RS24165"
                     /old_locus_tag="pgaptmp_004833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003878599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LexA family transcriptional regulator"
                     /protein_id="HGU4550603.1"
                     /translation="MTTLTQCQQQVLDMLISYQKERGFPPTNQEVAAMLGYRSVNAAV
                     EHLRALEKKGVITIKRGVARGITLHTTVKDDDSEAAGIIRALLAGEENARLRAAHWLH
                     ERGLKV"
     gene            803..1192
                     /locus_tag="ACHOSC_RS24170"
                     /old_locus_tag="pgaptmp_004834"
     CDS             803..1192
                     /locus_tag="ACHOSC_RS24170"
                     /old_locus_tag="pgaptmp_004834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000767134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RusA family crossover junction
                     endodeoxyribonuclease"
                     /protein_id="HGU4550604.1"
                     /translation="MKLILPFPPSVNTYWRHPNKGAFAGKSLISAAGRKFQSAACAAI
                     VEQLRRLPKPTSAPASVEIVLFPPDNRIRDLDNYNKALFDALTHAGVWEDDRQVKRML
                     VEWGPVIPKGKVEITISKYEKPAGAAA"
     gene            1212..2009
                     /locus_tag="ACHOSC_RS24175"
                     /old_locus_tag="pgaptmp_004835"
     CDS             1212..2009
                     /locus_tag="ACHOSC_RS24175"
                     /old_locus_tag="pgaptmp_004835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001061418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005634 - nucleus [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="KilA-N domain-containing protein"
                     /protein_id="HGU4550605.1"
                     /translation="MNNLMVIDGIEVRRDAYGRYSLNDLHRAAGSLDKHKPAFWLRNE
                     QTERLISELQICNSVNIEPVNVIRGGNNQGTYVCKELVYAYAMWISPSFHLKVIRTFD
                     MVTSAPEKLSGQAADKMQAGVILLDFMRRELNLSNSSVLGACQKLQEAVGLPNLAPRY
                     AIDAPADAPDGSSRPTLSLSALLKQYGIRLTANQAYHQMVKLGIVEQRERYSRTAINN
                     IKKFWSLTAKGCMFGKNITSPANPRETQPHFFESRFPELLKLLDTVH"
BASE COUNT          511 a          513 c          609 g          452 t
ORIGIN      
        1 ttaaatgacg ttcagaaatt cattgaagcc catccggggt gtacttccgg agacattgcg
       61 gatgcttttg caggttactc acggcagcgc gttctgcagt ctgcaagcaa gttacgtcag
      121 agtgggcgtg tggctcaccg ttgtgaagga gatacacgca gacatttccc gcgcctgact
      181 gagagagcgc aggagccgga accacaacca gttcgtgaaa ccagacctgt gcgcaatttc
      241 tatgtcggca ctaacgatcc ccgggtgatt ttgtgcctga cccgccaggc ggaagaactg
      301 gagtccaggg gcttataccg tcgtgctgca acggtgtgga tggcggcatt ccgtgaaagc
      361 cactcccagc cagaacgaaa caattttctg gcgcgtcgtg agcagtgttt acggaaaagc
      421 agcaagcgcg ctgtatcggg tgatgagtgg tatctgtcag ggaattacgt gggggcttaa
      481 tgacgacgtt aactcaatgc cagcagcagg tgctggatat gctgatttct tatcagaaag
      541 aacgtggttt cccgccaacc aatcaggagg tggcagccat gctgggatac cgttcggtga
      601 atgcagcggt ggagcatctt cgcgcactgg agaaaaaagg cgtcatcacg ataaagcgtg
      661 gcgtggcccg gggtatcacg cttcatacca cagtgaagga cgacgacagc gaggcagccg
      721 ggattatccg cgcactgctt gccggtgagg aaaacgccag actgcgtgca gcccactggt
      781 tacatgagag gggcctgaaa gtatgaagct gatcctgcct ttcccgccca gcgtgaacac
      841 gtactggcga caccccaaca aaggggcgtt tgctggtaag agtctgataa gcgcagcggg
      901 gcgcaaattc cagagcgcgg catgcgcagc aatagttgag cagttacgtc gtctgccgaa
      961 accaacgtcg gcacctgctt cagtggagat cgtgttgttt cctccggata accggatccg
     1021 cgatctggac aactataaca aggcgctgtt tgacgccctg acccacgcgg gtgtgtggga
     1081 agacgacaga caggtgaaaa gaatgctggt ggagtgggga ccggttatcc cgaaagggaa
     1141 ggtcgagatc actatcagta agtatgagaa accggcgggt gcagccgcct gattaagagg
     1201 agaaacgaag tatgaataat ctgatggtta ttgatggtat tgaagttcgt cgtgatgctt
     1261 atgggcgtta cagcctgaac gatctgcaca gggctgctgg ttctctggat aagcataagc
     1321 ctgcattctg gctccgcaat gagcaaactg agcgtttaat aagcgagttg cagatttgca
     1381 actcggtcaa tatagagcca gttaacgtta ttcgtggcgg aaataaccag gggacgtatg
     1441 tctgcaaaga actggtgtat gcctatgcaa tgtggatcag cccgtcattc catctgaagg
     1501 tgatccgtac tttcgacatg gtaaccagcg caccggaaaa attatccgga caggctgctg
     1561 acaagatgca ggctggtgtg atcctgctgg actttatgcg ccgggaatta aacctgtcta
     1621 actcatcagt gcttggagcc tgtcagaagc ttcaggaggc tgttggctta ccgaatctgg
     1681 caccgcgcta tgccattgat gctcctgctg acgcgcctga tggctcaagc cgccccacgc
     1741 tgtcactgag tgcactgctg aagcagtatg gtatccgcct gacagctaat caggcatatc
     1801 accagatggt gaagctgggg atcgtcgagc agcgcgaacg atacagccgt accgcgatta
     1861 acaacatcaa aaaattctgg tcgctgacag cgaaaggctg catgttcggc aagaatatca
     1921 ccagtcccgc aaatccgcgc gagacgcagc cgcatttctt cgaatcccga ttccctgagc
     1981 tgttaaagct gctcgatacc gttcattgag gtgaccgtga gagcactact gacccctgaa
     2041 attgccccgc gtatggggat cgtattgttc aggccaggtt cagag
//
LOCUS       DAZKHF020000426         2072 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2201S31241
            SAMN43267800-rid25106413.denovo.428, whole genome shotgun sequence.
ACCESSION   DAZKHF020000426 DAZKHF020000000
VERSION     DAZKHF020000426.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267800
            Sequence Read Archive: SRR30307263
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2072)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2072)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 2072)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350581.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350581.2
            Genome Coverage       :: 151x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:21:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,652
            CDSs (total)                      :: 5,557
            Genes (coding)                    :: 5,320
            CDSs (with protein)               :: 5,320
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 237
            CDSs (without protein)            :: 237
            Pseudo Genes (ambiguous residues) :: 0 of 237
            Pseudo Genes (frameshifted)       :: 102 of 237
            Pseudo Genes (incomplete)         :: 130 of 237
            Pseudo Genes (internal stop)      :: 52 of 237
            Pseudo Genes (multiple problems)  :: 41 of 237
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2072
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267800-rid25106413.denovo.428"
                     /serotype="O 26:H11"
                     /isolate="2201S31241"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-01"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            16..207
                     /gene="hemP"
                     /locus_tag="ACHOSC_RS24180"
                     /old_locus_tag="pgaptmp_004836"
     CDS             16..207
                     /gene="hemP"
                     /locus_tag="ACHOSC_RS24180"
                     /old_locus_tag="pgaptmp_004836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemin uptake protein HemP"
                     /protein_id="HGU4550606.1"
                     /translation="MRYTDSRKLTPETDANHKTASPQPIRRISSQTLLGPDGKLIIDH
                     DGQEYLLRKTQAGKLLLTK"
     gene            complement(211..1647)
                     /gene="selO"
                     /locus_tag="ACHOSC_RS24185"
                     /old_locus_tag="pgaptmp_004837"
     CDS             complement(211..1647)
                     /gene="selO"
                     /locus_tag="ACHOSC_RS24185"
                     /old_locus_tag="pgaptmp_004837"
                     /EC_number="2.7.7.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0070733 - AMPylase activity [Evidence
                     IEA];
                     GO_process: GO:0018117 - protein adenylylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein adenylyltransferase SelO"
                     /protein_id="HGU4550607.1"
                     /translation="MTLSFITRWRDELPATYTALSPTPLNNARLIWHNTELANTLSIP
                     SSLFKNGAGVWGGETLLPGMSPLAQVYSGHQFGVWAGQLGDGRGILLGEQLLADGTTM
                     DWHLKGAGLTPYSRMGDGRAVLRSTIRESLASEAMHYLGIPTTRALSIVTSDSPVYRE
                     TVEPGAMLMRVAPSHLRFGHFEHFYYRREPEKVRQLADFAIRHYWSYLEDDEDKYRLW
                     FSDVVARTASLIAQWQTVGFAHGVMNTDNMSLLGLTLDYGPFGFLDDYEPGFICNHSD
                     HQGRYSFDNQPAVALWNLQRLAQTLSPFVAVDALNEALDSYQQVLLTHYGQRMRQKLG
                     FMTEQKEDNALLNELFSLMARERSDYTRTFRMLSLTEQHSAASPLRDEFIDRAAFDDW
                     FARYRGRLQQDEVSDSERQQLMQSVNPALVLRNWLAQRAIEAAEKGDMTELHRLHEAL
                     RNPFSDRADDYVSRPPDWGKRLEVSCSS"
     gene            complement(1710..>2072)
                     /gene="ydiV"
                     /locus_tag="ACHOSC_RS24190"
                     /old_locus_tag="pgaptmp_004838"
     CDS             complement(1710..>2072)
                     /gene="ydiV"
                     /locus_tag="ACHOSC_RS24190"
                     /old_locus_tag="pgaptmp_004838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anti-FlhDC factor"
                     /protein_id="HGU4550608.1"
                     /translation="YPHLNEGKDNRDLLSLSQMYPLVLGNLGAGNSTMKAVFDGLFTR
                     VMLDKSFIQQQITHRSFEPFIRAIQAQISPCCNCIIAGGIDTTEILAQITPFDFHALQ
                     GCLWPAVPINQITTLVQR"
BASE COUNT          545 a          559 c          488 g          480 t
ORIGIN      
        1 ttattattaa tttttatgcg ttatacggat agcagaaaac tcacgcctga aacggatgcc
       61 aatcacaaga ccgcttcccc gcagcctatt cggcgaattt ccagccagac actgttaggt
      121 ccggatggca aactgattat cgatcatgac gggcaagaat atctgctccg taaaactcag
      181 gctggcaagc tgctgttgac caagtagcct ttaactcgag cagctgactt ccagccgttt
      241 accccagtca ggtggacgac tgacatagtc atcagctctg tcgctgaaag gatttcgcaa
      301 cgcctcatgc aggcggtgca attccgtcat atcacccttt tctgccgctt caatcgcccg
      361 ttgcgccaac cagttgcgca acaccagagc tgggttaacg ctttgcatca gctgctgacg
      421 ctcactatcg ctaacctcgt cttgttgcaa acgcccccga taacgggcaa accagtcatc
      481 aaatgccgca cgatcaataa actcatcacg tagcggtgac gccgcgctgt gctgctcggt
      541 cagactcagc atgcggaatg tgcgggtata atcgctgcgc tctcgcgcca tcagactgaa
      601 taattcattc agtagcgcgt tatcctcttt ttgctccgtc atgaagccca gtttctgccg
      661 catccgttgt ccataatgcg tcaacaaaac ctgctgatag ctgtccaggg cctcattcag
      721 ggcatctacg gcaacaaatg gtgacaatgt ctgcgccaga cgctgtaaat tccacaacgc
      781 gacagcaggt tgattatcaa agctataacg cccttgatga tccgagtgat tacaaataaa
      841 accgggttcg taatcatcca gaaaaccaaa cggcccgtaa tcaagcgtca gccccagcag
      901 cgacatgtta tcggtattca tcaccccatg agcaaagccg accgtctgcc attgggcaat
      961 taacgatgcg gtacgtgcga caacatcgct aaaccagagg cggtatttgt cctcatcatc
     1021 ttcaagatat gaccagtaat gacgaatggc aaagtcagct aactgacgaa ccttttccgg
     1081 ctcgcggcga tagtaaaaat gttcgaaatg accaaagcgc agatgacttg gtgccacacg
     1141 catcagcatc gcgcctggct ccaccgtttc ccgatacact ggcgaatcgc tggtgacgat
     1201 acttaacgcg cgggtcgtcg gaatgcccag ataatgcatc gcctcactgg cgagactttc
     1261 tcgtatcgtc gaacgtaaaa ccgcccgtcc atcacccatt cgcgaataag gcgtcaggcc
     1321 agcacctttc agatgccagt ccattgtagt gccatcagca agcagttgtt cgccgagtaa
     1381 aataccgcgc ccatcaccca gttggcccgc ccagacgccg aactgatgac cactgtaaac
     1441 ctgcgccagt ggtgacatac caggcagtag ggtttcgccg ccccagacac ctgcgccatt
     1501 tttaaacagc gacgatggaa tactcagcgt gttagcgagt tcggtattat gccaaatcag
     1561 ccgggcatta tttaaaggcg taggggaaag tgctgtatag gttgctggca attcatcgcg
     1621 ccagcgggta ataaaagaca gggtcataga tcctcctgtt taatagtgta gacggttact
     1681 ctcgtcttaa acaccagcaa acaggagggt tatcgctgaa ccaacgtcgt tatctgattt
     1741 ataggaacgg caggccacag gcatccctgg agagcatgga aatcgaaggg ggtaatctgt
     1801 gccaatattt ctgtcgtatc aatgcccccc gcgatgatgc aattgcaaca aggggaaatt
     1861 tgcgcctgga tcgcacgaat aaacggttcg aaggagcgat gcgtgatttg ttgctgaata
     1921 aaacttttat ccagcatgac gcgggtgaaa agaccatcaa aaaccgcttt cattgtgcta
     1981 ttacctgctc ccaaattgcc taacacaagg ggatacattt gcgataagga taaaagatcg
     2041 cgattatctt ttccttcatt gagatgcggg ta
//
LOCUS       DAZKHF020000427         2054 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2201S31241
            SAMN43267800-rid25106413.denovo.429, whole genome shotgun sequence.
ACCESSION   DAZKHF020000427 DAZKHF020000000
VERSION     DAZKHF020000427.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267800
            Sequence Read Archive: SRR30307263
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2054)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2054)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 2054)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350581.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350581.2
            Genome Coverage       :: 151x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:21:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,652
            CDSs (total)                      :: 5,557
            Genes (coding)                    :: 5,320
            CDSs (with protein)               :: 5,320
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 237
            CDSs (without protein)            :: 237
            Pseudo Genes (ambiguous residues) :: 0 of 237
            Pseudo Genes (frameshifted)       :: 102 of 237
            Pseudo Genes (incomplete)         :: 130 of 237
            Pseudo Genes (internal stop)      :: 52 of 237
            Pseudo Genes (multiple problems)  :: 41 of 237
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2054
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267800-rid25106413.denovo.429"
                     /serotype="O 26:H11"
                     /isolate="2201S31241"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-01"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            <1..380
                     /locus_tag="ACHOSC_RS24195"
                     /old_locus_tag="pgaptmp_004839"
     CDS             <1..380
                     /locus_tag="ACHOSC_RS24195"
                     /old_locus_tag="pgaptmp_004839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309619.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HGU4550609.1"
                     /translation="MFNVSPIFLQEYSNSTYSNFSEASRAFLTMTMRPWLANFEQQIK
                     NALLVASPVPGIRYQVEFDSADLLRATPGERFATYERGIKSGVMCPNEAREREGLSPR
                     DGGDEFSQAWKQEVKISEGEKPE"
     gene            377..715
                     /locus_tag="ACHOSC_RS24200"
                     /old_locus_tag="pgaptmp_004840"
     CDS             377..715
                     /locus_tag="ACHOSC_RS24200"
                     /old_locus_tag="pgaptmp_004840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage head closure protein"
                     /protein_id="HGU4550610.1"
                     /translation="MNIGRLRDRVTIQTLKQTRAMTGEILETWEDGHTLWASVNMVSS
                     KEAISSGAELAIGTVRIWIRYRKDINATCRIKVSTGPLAGRVLNIIGQPLPDAARTRL
                     EILCREGAEK"
     gene            712..1008
                     /locus_tag="ACHOSC_RS24205"
                     /old_locus_tag="pgaptmp_004841"
     CDS             712..1008
                     /locus_tag="ACHOSC_RS24205"
                     /old_locus_tag="pgaptmp_004841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0016032 - viral process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="head-tail connector protein"
                     /protein_id="HGU4550611.1"
                     /translation="MTEELITLEEVKLHCRIDGDEEDQLISGYIAASLEACQIHIGRR
                     FDDGLEFTPAIKIGCMMFIAHLYENRQIVADNAKTHVPMTIGALWTAYRDVGVY"
     gene            1008..1448
                     /locus_tag="ACHOSC_RS24210"
                     /old_locus_tag="pgaptmp_004842"
     CDS             1008..1448
                     /locus_tag="ACHOSC_RS24210"
                     /old_locus_tag="pgaptmp_004842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease signature motif containing
                     protein"
                     /protein_id="HGU4550612.1"
                     /translation="MPWQPLRRCTEPGCNRRVKSGKCEEHRRSARQQQDSRRGSSRER
                     GYTRQWEKYRAMYLSKNPLCAHCLEKGIYTPAVVVDHIIPIDGGNDVLFWPEWNHQPL
                     CQACHNQKTKWLDPSTKNKRAAGGFREEEERAANRNNWMYGADE"
     gene            1432..1614
                     /locus_tag="ACHOSC_RS24215"
                     /old_locus_tag="pgaptmp_004843"
     CDS             1432..1614
                     /locus_tag="ACHOSC_RS24215"
                     /old_locus_tag="pgaptmp_004843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4550613.1"
                     /translation="MALMNEREQNRLIRGLIRQRDAWKAQETGHKDKASGRAERITAT
                     RLNDRDREVMECFRNR"
     gene            1737..>2054
                     /locus_tag="ACHOSC_RS24220"
                     /old_locus_tag="pgaptmp_004844"
     CDS             1737..>2054
                     /locus_tag="ACHOSC_RS24220"
                     /old_locus_tag="pgaptmp_004844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage terminase small subunit P27 family"
                     /protein_id="HGU4550614.1"
                     /translation="MARPPKAPAYLDDIAVKQWREKSRQLAERGDLTPADWSNLELYC
                     VNYSIYRKAVADLAARGFSIVNSQGGESRNPALSAKSDAERVMIKMASLLGFDPISRR
                     KNPP"
BASE COUNT          560 a          512 c          608 g          374 t
ORIGIN      
        1 gaatgttcaa cgtctcgccg atttttctgc aggaatacag caacagcacc tacagcaatt
       61 tcagcgaggc aagccgcgca tttctcacca tgacaatgcg cccgtggctg gcgaactttg
      121 agcagcagat aaaaaacgcc ctgctggtgg cctcgcctgt acctggtatc cggtatcagg
      181 tggagtttga cagcgcggac ctgttacggg ccacacctgg cgaacgcttt gccacctatg
      241 aacgcggcat caaatccggc gttatgtgcc cgaacgaagc ccgcgaacgt gaagggctgt
      301 ccccgcgtga tggtggtgat gagttcagcc aggcatggaa acaggaagta aaaatcagcg
      361 agggagaaaa accggaatga acatagggcg actgcgtgac agggtaacga ttcagaccct
      421 gaaacagacc agagccatga cgggcgaaat actcgaaacg tgggaggacg gtcacacact
      481 ctgggcaagc gtgaacatgg tcagcagcaa ggaggccatt tcatcgggtg cagagctggc
      541 gattggtacc gtaaggatct ggatacggta ccggaaggac atcaacgcca cctgccggat
      601 aaaggtcagt acggggccgc tggcggggcg tgtactgaat atcatcgggc agccgctgcc
      661 ggatgccgcc aggacacgcc ttgaaattct ttgtcgtgag ggcgcggaaa aatgacagaa
      721 gaacttatca ccctggaaga agtgaaactc cattgccgca tcgatggcga cgaggaagac
      781 cagttaatca gcggatacat tgccgcatcg cttgaggcgt gccagataca cataggcagg
      841 cgctttgatg acgggctgga gttcacgcca gccataaaga ttggctgcat gatgtttatc
      901 gctcacctgt atgagaatcg ccagattgtc gcggataacg caaaaacaca cgtacccatg
      961 acgattggcg cactctggac ggcttaccgt gatgtggggg tgtactgatg ccgtggcaac
     1021 cattaagacg atgtacagaa ccaggctgta acaggcgcgt taaatccggc aagtgtgagg
     1081 agcacagacg cagcgcacgc cagcagcagg acagccggag aggcagcagc agggagcgag
     1141 ggtatacgag gcagtgggag aaataccgcg ccatgtatct gagtaaaaac ccgttatgtg
     1201 cgcattgcct ggaaaaagga atatacacgc ccgccgtggt ggtggatcac attatcccaa
     1261 tcgatggcgg taatgatgtt ctcttctggc ctgagtggaa tcatcagcca ttgtgccagg
     1321 cgtgtcataa ccagaaaacg aaatggcttg atccgtccac aaaaaacaag cgtgctgcgg
     1381 gtggattccg tgaggaggaa gaacgggccg ctaaccgcaa taactggatg tatggcgctg
     1441 atgaatgagc gggaacaaaa ccgccttatc cgtggactta taaggcagcg tgacgcatgg
     1501 aaggcacagg aaacagggca taaagataaa gcatcaggac gcgcagaacg catcacagcg
     1561 acgcgattaa acgaccgtga ccgcgaggtt atggaatgtt tccgcaatcg ctgatgaggc
     1621 tgtatgacgg ggtgggggga gttttcagga cgaacccgac cccgcccaga accgaacgcc
     1681 tcctcaaatt tttatgcacg ggaatttttt gaaaaataat ctgacgaaaa ataagcatgg
     1741 caagaccacc gaaagccccc gcctacctgg atgacatcgc cgtgaagcag tggcgggaaa
     1801 aatcgcggca gcttgcggaa cggggagacc tgacccccgc cgactggagc aatctggaac
     1861 tgtattgtgt caactactcc atttaccgga aagccgtcgc agaccttgcg gcgcgcgggt
     1921 tcagcattgt taacagtcag ggcggcgaga gcagaaaccc cgcattaagc gcgaaatcag
     1981 acgctgaaag ggtgatgata aaaatggcct ccttgctcgg ttttgacccg ataagccgcc
     2041 gtaaaaatcc accg
//
LOCUS       DAZKHF020000428         2053 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2201S31241
            SAMN43267800-rid25106413.denovo.430, whole genome shotgun sequence.
ACCESSION   DAZKHF020000428 DAZKHF020000000
VERSION     DAZKHF020000428.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267800
            Sequence Read Archive: SRR30307263
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2053)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2053)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 2053)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350581.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350581.2
            Genome Coverage       :: 151x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:21:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,652
            CDSs (total)                      :: 5,557
            Genes (coding)                    :: 5,320
            CDSs (with protein)               :: 5,320
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 237
            CDSs (without protein)            :: 237
            Pseudo Genes (ambiguous residues) :: 0 of 237
            Pseudo Genes (frameshifted)       :: 102 of 237
            Pseudo Genes (incomplete)         :: 130 of 237
            Pseudo Genes (internal stop)      :: 52 of 237
            Pseudo Genes (multiple problems)  :: 41 of 237
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2053
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267800-rid25106413.denovo.430"
                     /serotype="O 26:H11"
                     /isolate="2201S31241"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-01"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            <1..170
                     /locus_tag="ACHOSC_RS24225"
                     /old_locus_tag="pgaptmp_004845"
     CDS             <1..170
                     /locus_tag="ACHOSC_RS24225"
                     /old_locus_tag="pgaptmp_004845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0020002 - host cell plasma membrane
                     [Evidence IEA];
                     GO_function: GO:0042802 - identical protein binding
                     [Evidence IEA];
                     GO_function: GO:0140911 - pore-forming activity [Evidence
                     IEA];
                     GO_process: GO:0019835 - cytolysis [Evidence IEA];
                     GO_process: GO:0044659 - viral release from host cell by
                     cytolysis [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="class II holin family protein"
                     /protein_id="HGU4550615.1"
                     /translation="AVGTGYWLLQLLDKVSPSQWVAIGVLGSLLFGLLTYLTNLYFKI
                     REDRRKVARGE"
     gene            complement(426..698)
                     /locus_tag="ACHOSC_RS24230"
                     /old_locus_tag="pgaptmp_004846"
     CDS             complement(426..698)
                     /locus_tag="ACHOSC_RS24230"
                     /old_locus_tag="pgaptmp_004846"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309125.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4550616.1"
                     /translation="MTENVKSELLLLMADNNEATSSILADPYGKISHKTLDIITTTLT
                     PLMLQRLKHNINAWVNEELSPPCLWDSRYACQQKMRIFNLLSPKLR"
     gene            858..1391
                     /locus_tag="ACHOSC_RS24235"
                     /old_locus_tag="pgaptmp_004847"
     CDS             858..1391
                     /locus_tag="ACHOSC_RS24235"
                     /old_locus_tag="pgaptmp_004847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysozyme"
                     /protein_id="HGU4550617.1"
                     /translation="MNEKIKYGLSAAVLALIGAGASAPEILDQFLDEKEGNHTTAYRD
                     GAGIWTICRGAILVDGKPVIPGMKLSKEKCDRVNAIERDKALAWVEKNIRVPLTEPQK
                     AGIASFCPYNIGPGKCFPSTFYKRINAGDRKGACEAIRWWIKDGGRDCRIRSNNCYGQ
                     VSRRDQESALACWDIDR"
     gene            1612..1725
                     /locus_tag="ACHOSC_RS24240"
                     /old_locus_tag="pgaptmp_004848"
     CDS             1612..1725
                     /locus_tag="ACHOSC_RS24240"
                     /old_locus_tag="pgaptmp_004848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4550618.1"
                     /translation="MKGIEVETPASLDLTRAAAFAIRIVAIAVLVWAIRWW"
     gene            1727..1990
                     /locus_tag="ACHOSC_RS24245"
                     /old_locus_tag="pgaptmp_004849"
                     /pseudo
     CDS             1727..1990
                     /locus_tag="ACHOSC_RS24245"
                     /old_locus_tag="pgaptmp_004849"
                     /inference="COORDINATES: protein motif:HMM:NF015218.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_process: GO:0019835 - cytolysis [Evidence IEA];
                     GO_process: GO:0044659 - viral release from host cell by
                     cytolysis [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="lysis system i-spanin subunit Rz"
     gene            1947..>2053
                     /locus_tag="ACHOSC_RS24250"
                     /old_locus_tag="pgaptmp_004850"
     CDS             1947..>2053
                     /locus_tag="ACHOSC_RS24250"
                     /old_locus_tag="pgaptmp_004850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4550619.1"
                     /translation="MRELKMKLCVLMLPLVVSACGSTPPAPVPCVKPPAP"
BASE COUNT          529 a          416 c          568 g          540 t
ORIGIN      
        1 cagcggtggg gacgggatac tggttactgc agctgctgga caaagtctct ccgtcccagt
       61 gggtggcaat aggtgtgctg ggaagtctgc tgtttggcct gctgacgtat ctgactaacc
      121 tgtatttcaa aatcagagag gaccgtcgta aggtggcgcg gggagagtag tcgatgaata
      181 aacaatacga actggttgta aaatgaatat ttctaactga aaaaacgttc catgaggtaa
      241 gaaaaggtca caggcaatca ataacaggac gtgatgaaag acccttgcat ttgtgcgctt
      301 tctctttaga tagcagcaga tactgaaaat ctgagttgtc ggggagtcag ggatacagct
      361 gtgcaagagt tggtcattgt gattccattg aaatcctgta tgccatgaag ggcaggattt
      421 tatggctacc tgagctttgg tgatagtaag ttgaaaattc gcattttttg ctgacatgcg
      481 taacgagaat cccataagca gggaggactt aattcttcat taacccatgc gttgatatta
      541 tgtttcagcc gttgaagcat cagcggtgtt aatgttgtgg taataatatc cagcgtttta
      601 tgtgagatct taccgtaagg gtctgcaaga atgctgcttg ttgcttcgtt attatctgcc
      661 atcagaagaa gtaactctga tttaacgttt tctgtcatta gttgtaaaaa tcttctgcgc
      721 aaactttctt tactgttcat ttatatggct tcatttgttg taatctgctg cgtctcaagg
      781 gatatgttta tgagagcgac catgagtgtt ggattatata cctaacatat caagggatta
      841 gaaatcgata aatccccatg aacgaaaaaa taaaatacgg cctgtcggct gccgttctgg
      901 cgctgattgg tgcaggtgct tctgcgcctg aaatcctcga ccagtttctg gatgaaaagg
      961 aaggtaacca caccacggca taccgtgatg gtgcggggat ctggaccatc tgccgtggtg
     1021 ccattctggt ggatggtaag cctgttattc ctggcatgaa gctgtcaaag gaaaaatgcg
     1081 accgggttaa tgccatcgaa cgtgacaagg cgctggcatg ggtggagaaa aacatccggg
     1141 tgccgctgac cgaaccccag aaagcgggga tcgcgtcatt ctgtccgtac aacattggcc
     1201 ccggtaagtg cttcccgtcg acgttttata aacgaattaa tgcaggcgat cgaaaaggtg
     1261 cctgtgaggc gattcgctgg tggattaagg acggtggcag agactgccgt atccgttcaa
     1321 ataattgcta cggtcaggtc tcacggcgtg accaggagag cgcgctggcg tgctgggaca
     1381 tcgacagata gcagaatatt ttcctgaaaa atgacgttgg ccaacgcggg tggataacac
     1441 gaaatcctga aaactggtaa aacctaagtg aataaaagta aaaaccccgt ttgttggcag
     1501 caagcggggt tttgtgtttt ctgaccttga gcaaggcaag ggagaaatta tgggtaggga
     1561 ggtactttcc ctgtgaggaa gtataaaaga ttctttctga ggttgtccat tatgaaaggc
     1621 attgaagtgg agacgccagc cagtctggat ttaacaagag cggcagcttt tgccattcgt
     1681 attgtggcca ttgctgttct ggtctgggca atccgttggt ggtgatatga accgtgttct
     1741 gtgcgtggtt atcattgtcc tgctggtggc ctgtggtgcg cttagtctgg ggctgaatca
     1801 ttaccgtgat cacgccatca tctacaaaga gcagcgcgat aaaaaagcca gtgagctgga
     1861 gctggcgaac gcgacaatta ctgatatgca gatacgccag cgtgatgtcg ctgcacttga
     1921 tgccagatac tcgagggaat tagccgatgc gagagctgaa aatgaaactc tgcgtgctga
     1981 tgttgccgct ggtcgtaagc gcctgcggat caacgccacc tgctccggta ccgtgcgtga
     2041 agccaccggc acc
//
LOCUS       DAZKHF020000429         2033 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2201S31241
            SAMN43267800-rid25106413.denovo.431, whole genome shotgun sequence.
ACCESSION   DAZKHF020000429 DAZKHF020000000
VERSION     DAZKHF020000429.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267800
            Sequence Read Archive: SRR30307263
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2033)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2033)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 2033)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350581.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350581.2
            Genome Coverage       :: 151x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:21:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,652
            CDSs (total)                      :: 5,557
            Genes (coding)                    :: 5,320
            CDSs (with protein)               :: 5,320
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 237
            CDSs (without protein)            :: 237
            Pseudo Genes (ambiguous residues) :: 0 of 237
            Pseudo Genes (frameshifted)       :: 102 of 237
            Pseudo Genes (incomplete)         :: 130 of 237
            Pseudo Genes (internal stop)      :: 52 of 237
            Pseudo Genes (multiple problems)  :: 41 of 237
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2033
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267800-rid25106413.denovo.431"
                     /serotype="O 26:H11"
                     /isolate="2201S31241"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-01"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            33..109
                     /locus_tag="ACHOSC_RS24255"
                     /old_locus_tag="pgaptmp_004851"
     tRNA            33..109
                     /locus_tag="ACHOSC_RS24255"
                     /old_locus_tag="pgaptmp_004851"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:67..69,aa:Arg,seq:tct)
     gene            200..1159
                     /locus_tag="ACHOSC_RS24260"
                     /old_locus_tag="pgaptmp_004852"
     CDS             200..1159
                     /locus_tag="ACHOSC_RS24260"
                     /old_locus_tag="pgaptmp_004852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Shiga toxin Stx2 subunit A"
                     /protein_id="HGU4550620.1"
                     /translation="MKCILFKWVLCLLLGFSSVSYSREFTIDFSTQQSYVSSLNSIRT
                     EISTPLEHISQGTTSVSVINHTPPGSYFAVDIRGLDVYQARFDHLRLIIEQNNLYVAG
                     FVNTATNTFYRFSDFTHISVPDVTTVSMTTDSSYTTLQRVAALERSGMQISRHSLVSS
                     YLALMEFSGNTMTRDASRAVLRFVTVTAEALRFRQIQREFRQALSETAPVYTMTPGDV
                     DLTLNWGRISNVLPEYRGEDGVRVGRISFNNISAILGTVAVILNCHHQGARSVRAVNE
                     ESQPECQITGDRPVIKINNTLWESNTAAAFLNRKSQFLYTTGK"
     gene            1171..1440
                     /locus_tag="ACHOSC_RS24265"
                     /old_locus_tag="pgaptmp_004853"
     CDS             1171..1440
                     /locus_tag="ACHOSC_RS24265"
                     /old_locus_tag="pgaptmp_004853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Shiga toxin Stx2a subunit B"
                     /protein_id="HGU4550621.1"
                     /translation="MKKMFMAVLFALVSVNAMAADCAKGKIEFSKYNEDDTFTVKVDG
                     KEYWTSRWNLQPLLQSAQLTGMTVTIKSSTCESGSGFAEVQFNND"
     gene            complement(1738..>2033)
                     /gene="iraM"
                     /locus_tag="ACHOSC_RS24270"
                     /old_locus_tag="pgaptmp_004854"
     CDS             complement(1738..>2033)
                     /gene="iraM"
                     /locus_tag="ACHOSC_RS24270"
                     /old_locus_tag="pgaptmp_004854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001295666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="anti-adapter protein IraM"
                     /protein_id="HGU4550622.1"
                     /translation="QPACDISFSAIWCNIKLILWYQSDIFLPPGCIFTPTRTGVILNN
                     KELPVTIYNVTPFNKNVWNLIKSSQECPANTGNITDKCFNHRCILEICPYGRK"
BASE COUNT          535 a          409 c          504 g          585 t
ORIGIN      
        1 agtcctcgat ggcggtccat tatcggtatt cagcgttgtt agctcagccg gacagagcaa
       61 ttgccttcta agcaatcggt cactggttcg aatccagtac aacgcgccat atttatttac
      121 caggctcgct tttgcgggcc ttttttatat ctgcgccggg tctggtgctg attacttcag
      181 ccaaaaggaa cacctgtata tgaagtgtat attatttaaa tgggtactgt gcctgttact
      241 gggtttttct tcggtatcct attcccggga atttacgata gacttttcga ctcaacaaag
      301 ttatgtctct tcgttaaata gtatacggac agagatatcg acccctcttg aacatatatc
      361 tcaggggacc acatcggtgt ctgttattaa ccacaccccg ccgggcagtt attttgctgt
      421 ggatatacga gggcttgatg tctatcaggc gcgttttgac catcttcgtc tgattattga
      481 gcaaaataat ttatatgtgg ccgggttcgt taatacggca acaaatactt tctaccgttt
      541 ttcagatttt acacatatat cagtgcccga tgtgacaacg gtttccatga caacggacag
      601 cagttatacc actctgcaac gtgtcgcagc gctggaacgt tccggaatgc aaatcagtcg
      661 tcactcactg gtttcatcat atctggcgtt aatggagttc agtggtaata caatgaccag
      721 agatgcatcc agagcagttc tgcgttttgt cactgtcacg gcagaagcct tacgcttcag
      781 gcagatacag agagaatttc gtcaggcact gtctgaaact gctcctgtgt atacgatgac
      841 gccgggagac gtggacctca ctctgaactg ggggcgaatc agcaatgtgc ttccggagta
      901 tcggggagag gatggtgtca gagtggggag aatatccttt aataatatat cagcgatact
      961 gggtactgtg gccgttatac tgaattgcca tcatcagggg gcgcgttctg ttcgcgccgt
     1021 gaatgaagag agtcaaccag aatgtcagat aactggcgac aggcccgtta taaaaataaa
     1081 caatacatta tgggaaagta atacagctgc agcgtttctg aacagaaagt cacagttttt
     1141 atatacaacg ggtaaataaa ggagttaagt atgaagaaga tgtttatggc ggttttattt
     1201 gcattagttt ctgttaatgc aatggcggcg gattgtgcta aaggtaaaat tgagttttcc
     1261 aagtataatg aggatgacac atttacagtg aaggttgacg ggaaagaata ctggaccagt
     1321 cgctggaatc tgcaaccgtt actgcaaagt gctcagttga caggaatgac tgtcacaatc
     1381 aaatccagta cctgtgaatc aggctccgga tttgctgaag tgcagtttaa taatgactga
     1441 ggcataacct gattcgtggt atgtgggtaa caagtgtaat ctgtgtcaca attcagtcag
     1501 ttgacagttg cctgtcagac tgagcatttg ttaaaaaaat ttcgcatggt gaatccccct
     1561 gtgcggaggg gcgactggtg tataaggtgt catctctgac gacaacctgg atcgagatac
     1621 gcgggttcag ggacaccgga ctgaactcac cgggaggcac ccggcaccat gttcatggtg
     1681 atacagaaat gcggcttcag cccctctccg gaggggcttt ttatctgaat gattctgtta
     1741 ttttcgcccg tatgggcata tttccagaat gcagcgatga ttaaaacact tatctgtgat
     1801 atttcctgtg tttgcagggc attcctggct gcttttaatt aaattccaga cgtttttatt
     1861 aaatggggtc acgttgtaaa tggtgacggg gagctcttta ttattgagta ttacgcctgt
     1921 ccgggttggt gtaaaaatac agcccggagg gaggaagata tcagactgat accataatat
     1981 taattttatg ttacaccata tggctgaaaa tgatatgtca catgctggct gga
//
LOCUS       DAZKHF020000430         2033 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2201S31241
            SAMN43267800-rid25106413.denovo.432, whole genome shotgun sequence.
ACCESSION   DAZKHF020000430 DAZKHF020000000
VERSION     DAZKHF020000430.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267800
            Sequence Read Archive: SRR30307263
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2033)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2033)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 2033)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350581.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350581.2
            Genome Coverage       :: 151x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:21:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,652
            CDSs (total)                      :: 5,557
            Genes (coding)                    :: 5,320
            CDSs (with protein)               :: 5,320
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 237
            CDSs (without protein)            :: 237
            Pseudo Genes (ambiguous residues) :: 0 of 237
            Pseudo Genes (frameshifted)       :: 102 of 237
            Pseudo Genes (incomplete)         :: 130 of 237
            Pseudo Genes (internal stop)      :: 52 of 237
            Pseudo Genes (multiple problems)  :: 41 of 237
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2033
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267800-rid25106413.denovo.432"
                     /serotype="O 26:H11"
                     /isolate="2201S31241"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-01"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            complement(336..770)
                     /gene="uspF"
                     /locus_tag="ACHOSC_RS24275"
                     /old_locus_tag="pgaptmp_004855"
     CDS             complement(336..770)
                     /gene="uspF"
                     /locus_tag="ACHOSC_RS24275"
                     /old_locus_tag="pgaptmp_004855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310024.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein UspF"
                     /protein_id="HGU4550623.1"
                     /translation="MNRTILVPIDISDSELTQRVISHVEAEAKIDDAEVHFLTVIPSL
                     PYYASLGLAYSAELPAMDDLKAEAKSQLEEIIKKFKLPTDRVHVHVEEGSPKDRILEL
                     AKKIPAHMIIIASHRPDITTYLLGSNAAAVVRHAECSVLVVR"
     gene            complement(911..2033)
                     /gene="ompN"
                     /locus_tag="ACHOSC_RS24280"
                     /old_locus_tag="pgaptmp_004856"
                     /pseudo
     CDS             complement(911..2033)
                     /gene="ompN"
                     /locus_tag="ACHOSC_RS24280"
                     /old_locus_tag="pgaptmp_004856"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415895.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="porin OmpN"
BASE COUNT          553 a          454 c          466 g          560 t
ORIGIN      
        1 ggcaaaaaaa gtgtagatta ttgtgcttta ttgtagttgt taatcctgcc taacatattg
       61 aaaaacataa aagttattgt tcgtcagata gttgcggttt ttactctgac ttgttcaggt
      121 tgtattgttc tttcttacta atttcttgat tttgcgacat ttaaaagcga ctcaattcgt
      181 tatatggcat cagaagagta tgcgtcatgc cggaacgccc agcataagaa atctgatata
      241 aaaactgtgg cgtgtatggt acggattaga ggggaaaatg tcagcacatt tgcgaaatga
      301 atcaaaaagc ccgcagcaat gtgcgggcgt tagtgtcagc gcacaaccag cacggagcac
      361 tctgcgtgac gcactacagc tgcggcgtta gaaccgagca gataagtggt gatatccggt
      421 cgatgggaag caatgatgat catatgagcg gggatcttct tcgccaattc cagaatgcgg
      481 tcttttggcg agccttcctc aacatggaca tgcactctgt cggttggcag tttaaatttt
      541 ttaatgatct cttccagttg cgatttggct tccgctttca ggtcatccat tgccggtaat
      601 tctgcggaat acgctaaacc cagagaggca tagtagggca gtgaaggtat taccgtcagg
      661 aaatgaacct ctgcatcatc aatctttgcc tctgcctcaa cgtggctaat cacgcgttga
      721 gttaattctg aatcggaaat atcgataggg acaagaatcg ttctgttcat aaaacctcct
      781 gttttagtat ccgcataaag tgtaacgcca gatgacactt tttgtgtaat gacggagttc
      841 acatttttaa tttagatcaa aggaggaaga ataagcagaa aaagcccgcc ataacagcgg
      901 gcaggaggat ttagaactga taaaccagac ctaaagcgac aatatcatcg gtagagatgc
      961 cgttggcagc gtagaaactg tcatcttcat ccaacaggtt gattttatag tcaacataag
     1021 tggacatgtt tttattgaaa tagtaagtcg cgccaacatc ggcgtattta accagatctt
     1081 tatcatcaac acctgccggg ttgtctgcac caccggcagc gtgcaggtca cggcctttag
     1141 acatcaggaa agagactgcc ggacgcagac caaaatcaaa ctggtactgt gcagtgactt
     1201 caaagttctg ggttttgttt gcaacagcat aatcgctgtc gccaaacggg gtcatattac
     1261 gcgtttctga atacatggtt gccaggtaaa tattgttagc atcgtatttt agcccagcag
     1321 tccacgcgtc tgctttatca ccacccgccg cagtatggtt aacctggtca ttggtgcggt
     1381 cagaagaggt gtatgccgca ccagcgctaa agcccatgcc taaatcatat gttgtggaaa
     1441 gaccccaccc cagccgtcac cgttttcatg gcgaacatca cgtccgttgt tggtgccttc
     1501 ctgaccatta ctggcacctt cgttgttacc ttgatactgc accgcgaagt tcagaccatt
     1561 taccagaccg aagaaatcag tattacgata agtcgcgacg ccattggctc gaccagtcat
     1621 aaagttgtct gcattggtat aagagtcacc gccaaattca ggcagcatat cggtccagcc
     1681 ttcgatgtcg tacattacgc cataattacg tccgtaatcg aaagaaccgt aatctgcaaa
     1741 tttcagtccg gcaaatgcca gacgggtcca tgactggttt tttgaagatt cagtgttgtt
     1801 tgcctgaata ttgtattccc attgaccgta gccagtaagt tgatcgttaa tttgggtttc
     1861 gcctttaaaa cccagacgcg catagctctg gtcgccatct ttcgctgaat tatcagaaaa
     1921 ataatgcagg ccatcaactt tgccatacag atctaatttg ttgccgtctt tattataaac
     1981 ttcggctgca tgtgcagcac ctgcggcgag cagggcagga attaaaagtg cca
//
LOCUS       DAZKHF020000431         2029 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2201S31241
            SAMN43267800-rid25106413.denovo.433, whole genome shotgun sequence.
ACCESSION   DAZKHF020000431 DAZKHF020000000
VERSION     DAZKHF020000431.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267800
            Sequence Read Archive: SRR30307263
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2029)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2029)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 2029)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350581.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350581.2
            Genome Coverage       :: 151x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:21:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,652
            CDSs (total)                      :: 5,557
            Genes (coding)                    :: 5,320
            CDSs (with protein)               :: 5,320
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 237
            CDSs (without protein)            :: 237
            Pseudo Genes (ambiguous residues) :: 0 of 237
            Pseudo Genes (frameshifted)       :: 102 of 237
            Pseudo Genes (incomplete)         :: 130 of 237
            Pseudo Genes (internal stop)      :: 52 of 237
            Pseudo Genes (multiple problems)  :: 41 of 237
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2029
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267800-rid25106413.denovo.433"
                     /serotype="O 26:H11"
                     /isolate="2201S31241"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-01"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            14..115
                     /locus_tag="ACHOSC_RS24285"
                     /old_locus_tag="pgaptmp_004857"
     CDS             14..115
                     /locus_tag="ACHOSC_RS24285"
                     /old_locus_tag="pgaptmp_004857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4550624.1"
                     /translation="MRTYNLNYLLPSQMQKCTCDFLYPAFDLCGGEA"
     gene            112..567
                     /gene="ybcN"
                     /locus_tag="ACHOSC_RS24290"
                     /old_locus_tag="pgaptmp_004858"
     CDS             112..567
                     /gene="ybcN"
                     /locus_tag="ACHOSC_RS24290"
                     /old_locus_tag="pgaptmp_004858"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA base-flipping protein YbcN"
                     /protein_id="HGU4550625.1"
                     /translation="MNLPQDGIKLHRGNFTAIGQQLQPYLEDGKCFRMVLKPWRERRS
                     LSQNALSHMWYSEISEYLIRRGKSFATAAWVKDALKHTYLGYETKDLVDVVTGEITTI
                     QSLRHTSDLDTGEMYVFLCKVEAWAMNIGCHLTIPQSCEFQLLRDKQEA"
     gene            567..737
                     /gene="ninE"
                     /locus_tag="ACHOSC_RS24295"
                     /old_locus_tag="pgaptmp_004859"
     CDS             567..737
                     /gene="ninE"
                     /locus_tag="ACHOSC_RS24295"
                     /old_locus_tag="pgaptmp_004859"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NinE family protein"
                     /protein_id="HGU4550626.1"
                     /translation="MATPLIRVMNGHIYRVPNRRKRKPELKPSEIPTLLGYTASLVDK
                     KWLRLAARRNHG"
     gene            730..1020
                     /gene="ybcO"
                     /locus_tag="ACHOSC_RS24300"
                     /old_locus_tag="pgaptmp_004860"
     CDS             730..1020
                     /gene="ybcO"
                     /locus_tag="ACHOSC_RS24300"
                     /old_locus_tag="pgaptmp_004860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1364 domain-containing protein"
                     /protein_id="HGU4550627.1"
                     /translation="MADLRKAARGRECQVRIPGVCNGNPETSVLAHIRLAGLCGTGIK
                     PPDLIATIACSACHDEIDRRTHFVDAEYAKECALEGMARTQVIWLKEGVIKA"
     gene            1017..1379
                     /gene="rusA"
                     /locus_tag="ACHOSC_RS24305"
                     /old_locus_tag="pgaptmp_004861"
     CDS             1017..1379
                     /gene="rusA"
                     /locus_tag="ACHOSC_RS24305"
                     /old_locus_tag="pgaptmp_004861"
                     /EC_number="3.1.21.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008821 - crossover junction DNA
                     endonuclease activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="crossover junction endodeoxyribonuclease RusA"
                     /protein_id="HGU4550628.1"
                     /translation="MNTYSITLPWPPSNNRYYRHNRGRTHVSAEGQAYRDNVARIIKN
                     AMLDIGLAMPVKIRIECHMPDRRRRDLDNLQKAAFDALTKAGFWLDDAQVVDYRVVKM
                     PVTKGGRLELTITEMGNE"
     gene            1376..1516
                     /gene="ylcG"
                     /locus_tag="ACHOSC_RS24310"
                     /old_locus_tag="pgaptmp_004862"
     CDS             1376..1516
                     /gene="ylcG"
                     /locus_tag="ACHOSC_RS24310"
                     /old_locus_tag="pgaptmp_004862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlcG family protein"
                     /protein_id="HGU4550629.1"
                     /translation="MMFEFYMAERLRHRWGRLRLYRFPGSVLTDYRILKNYAKTLTGA
                     GV"
     gene            1602..1985
                     /gene="quuD"
                     /locus_tag="ACHOSC_RS24315"
                     /old_locus_tag="pgaptmp_004863"
     CDS             1602..1985
                     /gene="quuD"
                     /locus_tag="ACHOSC_RS24315"
                     /old_locus_tag="pgaptmp_004863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antitermination protein QuuD"
                     /protein_id="HGU4550630.1"
                     /translation="MRDIQMVLERWGAWAANNHEDVTWSSIAAGFKGLITSKVKSRPQ
                     CCDDDAMIICGCMARLKKNNSDLHDLLVDYYVVGMTFMSLAGKHCCSDGYIGKRLQKA
                     EGIIEGMLMALDIRLEMDIVVNNSN"
BASE COUNT          534 a          462 c          528 g          505 t
ORIGIN      
        1 aacggggacg tttatgcgca catacaatct aaactatctt ctcccgtcac agatgcagaa
       61 atgcacctgc gattttcttt atccagcgtt tgacctctgc ggaggtgaag cgtgaacctc
      121 ccacaagacg gcatcaaact gcatcgcggt aacttcaccg ctatcggtca gcagctccag
      181 ccttatttgg aagacggcaa gtgctttcgc atggtgctta aaccgtggcg cgagagacgc
      241 agtctttccc agaatgcact tagccacatg tggtacagcg aaatcagtga ataccttatc
      301 cgcaggggga aatcgttcgc taccgcagca tgggtaaaag atgctctcaa acacacatac
      361 ctcggttatg aaaccaagga cctggttgat gtcgtaaccg gcgaaatcac tactatccag
      421 tcgttacgcc atacctccga tcttgatacc ggagagatgt atgtcttcct gtgtaaggtt
      481 gaagcctggg cgatgaatat tggctgccac ctgactattc cgcagagctg cgagttccag
      541 ctgctgcgcg acaagcagga ggcgtaatgg ctacaccgct tattcgtgtc atgaacggac
      601 acatctacag agtaccaaat cgtcgtaagc gtaagcctga gctaaaacca tccgaaatac
      661 caacactgct cggatatacc gccagcctgg ttgataaaaa atggttgcga ctggcagcaa
      721 ggaggaatca tggctgattt gagaaaagca gcgcgtggtc gcgaatgcca ggtaagaatc
      781 cctggcgtat gcaatggcaa ccctgaaacg tctgtactgg cacatatccg gctggctgga
      841 ctgtgtggca ccggtattaa accgccagac cttattgcca ccattgcctg ttctgcctgt
      901 cacgacgaaa tcgaccgccg cacgcatttt gtcgatgctg agtacgcaaa agaatgcgcg
      961 ctggaaggta tggcgagaac gcaggttatc tggctgaaag agggggtaat caaggcgtga
     1021 atacctacag catcacatta ccctggcctc cgagcaataa tcgctattac cgccataatc
     1081 gcgggcgcac gcacgtcagc gcagaggggc aggcataccg cgataacgtc gcccgaatca
     1141 ttaaaaacgc aatgctggat atcggcttgg ctatgcctgt gaaaatccgc attgagtgcc
     1201 acatgccgga tcgccgtcgc cgtgacctgg ataatctgca aaaagccgct tttgacgcac
     1261 tcactaaagc aggtttctgg ctggatgatg ctcaggtcgt tgattaccgc gttgtgaaga
     1321 tgcctgttac caaaggtggg aggctggaac tgaccatcac cgaaatgggg aatgaatgat
     1381 gtttgagttt tatatggcag aacgtcttcg ccaccgctgg gggcgtctgc gcttatatcg
     1441 tttccccggt tctgttttga ccgattaccg aatactgaag aattacgcca aaaccctgac
     1501 aggagcagga gtatgaagtc agagataaca atcaactaat actgtttcgt tgatttttgc
     1561 ttgtaattgg cgttctggtc tgatttttgt ggagtaagtt gatgcgtgat attcagatgg
     1621 ttcttgagcg ttggggagcg tgggcggcta ataatcatga agatgtgacc tggtcgtcca
     1681 ttgccgccgg ttttaaggga ttaattactt caaaagtaaa atctcgcccg caatgttgtg
     1741 acgatgacgc gatgatcatt tgcgggtgca tggcccgtct gaaaaagaac aacagcgatt
     1801 tgcacgattt attagtagat tattatgtag tcggtatgac attcatgtca ctggcaggta
     1861 agcattgctg ctctgatggt tatatcggga aaaggttaca gaaggctgag ggcataattg
     1921 aagggatgtt aatggcatta gatatccggt tagagatgga tatcgttgtt aataactcta
     1981 attaatatgc caattgttta ctaaaaatta ttaagaatgg ggcgttgaa
//
LOCUS       DAZKHF020000432         2018 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2201S31241
            SAMN43267800-rid25106413.denovo.435, whole genome shotgun sequence.
ACCESSION   DAZKHF020000432 DAZKHF020000000
VERSION     DAZKHF020000432.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267800
            Sequence Read Archive: SRR30307263
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2018)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2018)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 2018)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350581.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350581.2
            Genome Coverage       :: 151x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:21:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,652
            CDSs (total)                      :: 5,557
            Genes (coding)                    :: 5,320
            CDSs (with protein)               :: 5,320
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 237
            CDSs (without protein)            :: 237
            Pseudo Genes (ambiguous residues) :: 0 of 237
            Pseudo Genes (frameshifted)       :: 102 of 237
            Pseudo Genes (incomplete)         :: 130 of 237
            Pseudo Genes (internal stop)      :: 52 of 237
            Pseudo Genes (multiple problems)  :: 41 of 237
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2018
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267800-rid25106413.denovo.435"
                     /serotype="O 26:H11"
                     /isolate="2201S31241"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-01"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            165..917
                     /locus_tag="ACHOSC_RS24320"
                     /old_locus_tag="pgaptmp_004864"
     CDS             165..917
                     /locus_tag="ACHOSC_RS24320"
                     /old_locus_tag="pgaptmp_004864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000214382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septation initiation protein"
                     /protein_id="HGU4550631.1"
                     /translation="MTTVNQIPDANITPSLPPKIREAVDKVKAAKAVWQEERRKQTEA
                     AAMIETIRKRQEDTKTETQALNDEWRNLFRENQGNMTPRMKKLRAEIALGRETLDEFE
                     DLLAAQVAENEFLPWKTADAANHYISEHNRLIETHAVWLWNEFMKEHGQKLIQILGLL
                     KMTLGRSASAVIGVVHTVNDPESVLKQFISEQLTTPALSCNVSSTDDITLPGISIYAD
                     DKAIQDARQSPSPAARSRMLKQRDMVKGGEKK"
     gene            914..1468
                     /locus_tag="ACHOSC_RS24325"
                     /old_locus_tag="pgaptmp_004865"
     CDS             914..1468
                     /locus_tag="ACHOSC_RS24325"
                     /old_locus_tag="pgaptmp_004865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016232023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage polarity suppression protein"
                     /protein_id="HGU4550632.1"
                     /translation="MNAGTITQEALNDYRSAIKSWLTLRNTQSTYQLRLAALLDTEEK
                     PAAYASQLEDLRERLAVLEWQINCAARDGLYAHQIVLESCVTSATENFMSEHGDALTD
                     ALAPFLCAPYGLEAAMKILRTAVARQTEMRTPVISAAYKSIIDETGLTVDASMRADAS
                     ASFTPAQHKVFLARLNRLNEKGVC"
     gene            1470..1742
                     /locus_tag="ACHOSC_RS24330"
                     /old_locus_tag="pgaptmp_004866"
     CDS             1470..1742
                     /locus_tag="ACHOSC_RS24330"
                     /old_locus_tag="pgaptmp_004866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ogr/Delta-like zinc finger family protein"
                     /protein_id="HGU4550633.1"
                     /translation="MALKCPECGAVAHARTSAYEAPSVKRSWYQCQNLECSCTFTALE
                     SVDTIIMKPRCNEQESDKAKIPEKQQQTLNRYGSASKLSNRQQIPV"
BASE COUNT          575 a          492 c          503 g          448 t
ORIGIN      
        1 gtgaatagta tgtattaaag gaagcgtgta gatcagacat gacacctttc tctggctagc
       61 cagaacaagg tcattgttca gtcactgaca caatcctcct cggtagtgcg cttgtatgga
      121 ccccggcaca attgacacaa caacaaaaca ctaccggagc agtcatgact actgttaacc
      181 agatccctga tgcaaacatt acaccctccc tcccaccgaa aattcgcgaa gcggtggata
      241 aagttaaggc agcaaaagct gtctggcagg aagaacgacg aaaacaaact gaagctgctg
      301 caatgattga aactatccgt aaacgtcagg aagatacaaa aacggaaacg caggcgctta
      361 atgatgaatg gcgaaacctg tttcgtgaga atcagggaaa tatgacgcca cgcatgaaaa
      421 aactgcgggc agaaatcgcc ctcggacgcg aaacactgga tgagttcgaa gatttgctgg
      481 cagctcaggt ggcagaaaat gaattcctgc cctggaaaac tgcggatgct gcaaaccact
      541 acatcagcga acataatcgc ctgattgaaa ctcatgcagt gtggctctgg aatgagttta
      601 tgaaggaaca cggtcagaaa cttattcaga tccttggact gctgaaaatg actctgggcc
      661 gaagcgcttc tgctgttatc ggtgtagttc ataccgtaaa cgaccccgaa agtgtgctga
      721 agcaatttat cagcgagcaa ctcaccactc cggcactgtc ctgtaacgta tcttcaaccg
      781 atgatattac cctgccgggg atcagcattt atgcggacga taaagccatc caggatgcca
      841 gacaatcccc cagccctgca gcgcgttcac ggatgcttaa acagcgtgac atggttaaag
      901 ggggcgagaa gaaatgaatg ccggaaccat tactcaggaa gcactcaacg attaccgctc
      961 ggcgataaaa agctggctga cactacgtaa tacgcaaagt acctaccagc ttcgtctggc
     1021 ggctctgctg gatactgaag aaaaaccggc agcatatgcc agccagcttg aggatcttcg
     1081 tgagcgtctt gcagtcctcg aatggcagat taactgtgct gcacgggatg gtctttatgc
     1141 tcatcagatt gtgctggaga gttgtgttac aagcgcaacg gaaaacttca tgagcgagca
     1201 tggtgatgca ctcactgacg ctctggctcc ttttctttgc gctccatacg ggcttgaggc
     1261 ggcaatgaaa atattacgaa ccgctgtagc ccgacaaacg gaaatgcgta ctccggtaat
     1321 ttcagcagca tataagagca ttatcgacga aaccggatta acggtggatg catcaatgcg
     1381 cgctgatgct tcagccagtt tcaccccggc acaacataaa gttttcctgg cccgccttaa
     1441 ccgacttaat gaaaaaggag tgtgctgata tggccctgaa gtgtcctgaa tgtggcgcgg
     1501 tcgctcacgc cagaaccagc gcctatgaag ctccgtcggt taaacgctca tggtatcagt
     1561 gccagaatct ggaatgttcc tgcacattta ccgcactgga aagcgtggat acgataatta
     1621 tgaagcctcg gtgcaatgaa caggaatcag acaaagcaaa aataccggaa aaacaacagc
     1681 aaactctcaa tcgctatggc tccgcgtcaa agctgtcaaa ccgtcagcaa attcctgtct
     1741 gattagcaaa atacgaccac gaagtcccgg tccagtaccg ggatttttta cgcctttttc
     1801 ctggctggcc tgagagcgta tgagtgcata tctatggcgc atgaaaacgc atgagtctca
     1861 tgcgccattt ttgacgcgaa agcccttgtg tggtggcttc tgagacgatt tacggggtgc
     1921 atgaaaacca gtctgttaag cgaagcgggc aggcgggcgg ggcattgcgc gcaggaatgt
     1981 gtataccgtt ttagtaatga tcgctgacat ctcacttt
//
LOCUS       DAZKHF020000433         1966 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2201S31241
            SAMN43267800-rid25106413.denovo.436, whole genome shotgun sequence.
ACCESSION   DAZKHF020000433 DAZKHF020000000
VERSION     DAZKHF020000433.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267800
            Sequence Read Archive: SRR30307263
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1966)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1966)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 1966)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350581.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350581.2
            Genome Coverage       :: 151x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:21:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,652
            CDSs (total)                      :: 5,557
            Genes (coding)                    :: 5,320
            CDSs (with protein)               :: 5,320
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 237
            CDSs (without protein)            :: 237
            Pseudo Genes (ambiguous residues) :: 0 of 237
            Pseudo Genes (frameshifted)       :: 102 of 237
            Pseudo Genes (incomplete)         :: 130 of 237
            Pseudo Genes (internal stop)      :: 52 of 237
            Pseudo Genes (multiple problems)  :: 41 of 237
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1966
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267800-rid25106413.denovo.436"
                     /serotype="O 26:H11"
                     /isolate="2201S31241"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-01"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            complement(<1..1397)
                     /locus_tag="ACHOSC_RS24335"
                     /old_locus_tag="pgaptmp_004867"
     CDS             complement(<1..1397)
                     /locus_tag="ACHOSC_RS24335"
                     /old_locus_tag="pgaptmp_004867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415686.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HGU4550634.1"
                     /translation="MRNTLIPILVAICLFITGVAILNIQLWYSAKAEYLAGARYAANN
                     INHILEEASQATQTAVNIAGKECNLEEQYQLGTEAALKPHLRTIIILKQGIVWCTSLP
                     GNRVLLSRIPVFPDSNLLLAPAIDTVNRLPILLYQNQFADTRILVTISDQHIRGALNV
                     PLKGVRYVLRVADDIIGPTGDVMTLNGHYPYTEKVHSTKYHFTIIFNPPPLFSFYRLI
                     DKGFGILIFILLIACAAAFLLDRYFNKSATPEEILRRAINNGEIVPFYQPVVNGREGT
                     LRGVEVLARWKQPHGGYISPAAFIPLAEKSGLIVPLTQSLMNQVARQMNAIASKLPEG
                     FHIGINFSASHIISPTFVDECLNFRDSFTRRDLNLVLEVTEREPLNVDESLVQRLNIL
                     HENGFVIALDDFGTGYSGLSYLHDLHIDYIKIDHSFVGRVNADPESTRILDCVLDLAR
                     KLSISIVAEGVETNE"
     gene            complement(1729..1965)
                     /gene="ymgC"
                     /locus_tag="ACHOSC_RS24340"
                     /old_locus_tag="pgaptmp_004868"
     CDS             complement(1729..1965)
                     /gene="ymgC"
                     /locus_tag="ACHOSC_RS24340"
                     /old_locus_tag="pgaptmp_004868"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YmgC"
                     /protein_id="HGU4550635.1"
                     /translation="MPERFIFQCALFKNLEREVFMTHGYVDSHIIDQALRLRLKDETS
                     VILSDLYLQILQYIEMHKTTLTDIIINDRESVLS"
BASE COUNT          584 a          436 c          423 g          523 t
ORIGIN      
        1 tgttcattcg tttcgacacc ttcagcgacg atactgatcg aaagtttacg cgccagatcc
       61 aatacacaat ccagaattcg ggttgattct gggtctgcgt ttacgcggcc aacgaaactg
      121 tgatcaattt tgatataatc aatatgcagg tcatgaagat aagaaagccc tgagtagcca
      181 gtaccgaaat catccagcgc gatgacaaaa ccattttcat gcagaatgtt caaccgctga
      241 accagacttt catcaacatt caatggctca cgctcggtga cttccagaac aaggtttaaa
      301 tcgcggcggg taaaactgtc acggaaattt aaacactcgt cgacaaacgt cggcgaaata
      361 atatgcgaag cgctaaaatt aattccaata tgaaaacctt ccggcaattt actcgcgata
      421 gcgttcatct gtctggcaac ctgattcatc aggctttgcg taagcggaac gattaatccg
      481 gatttttcag caagtggaat aaatgccgcg ggtgatatat atccaccgtg aggttgtttc
      541 cagcgggcta acacctcaac tccccgcaat gtcccttccc gaccatttac cacaggttgg
      601 taaaaaggga cgatctcccc attatttatt gcccgtcgca ggatctcttc aggcgttgcg
      661 cttttattga aatatctatc aagcaggaag gcagcggcgc aggcgattaa cagaataaat
      721 attaaaatcc caaagccttt atcgataagt ctgtagaagc taaagagtgg cggcgggtta
      781 aagataatag tgaaatgata ttttgtggag tgaaccttct cggtataggg ataatgtcca
      841 ttaagcgtca tcacatcacc cgttggtcca ataatgtcat ccgccacgcg taatacatac
      901 cttacccctt tcaagggtac attaagtgcc ccacgaatat gctgatcgct tatcgtaacc
      961 aaaatgcgcg tatctgcaaa ttggttctga tagagcagga taggtaatct attaacggtg
     1021 tcgattgctg gagccaacag taaattactg tccgggaaaa caggaatacg agacaacagg
     1081 acccgattcc caggcaggga tgtacaccag actattccct gtttgagaat gatgattgtg
     1141 cgcaggtgag gtttcagagc tgcttcagtg ccaagctgat attgctcctc gaggttgcat
     1201 tccttcccgg caatgttaac cgctgtttga gtcgcttgtg acgcttcttc aagtatatga
     1261 ttgatattgt tggcggcata tctcgctccc gccaggtact ctgcttttgc ggaataccag
     1321 agctggatgt tcaaaatagc gacaccggta ataaacaagc atatcgcaac gaggatgggt
     1381 atgagtgtat tgcgcatttg cttgtcccgg ccaaagggtt taatacatga aaatgccatt
     1441 cattcgctcg cgctggcatc tacagagagc atggtagaga gcataagaaa tatccacgcc
     1501 gaagataaaa ttttctccat cttgatctaa cccattaatt attgagcata atgtaggcag
     1561 tacaaaataa gttaggcggg atatcaggcg tcaagaatgg agagcgagaa ctctccattc
     1621 ttgacacctg atattgcgga cataataaga aagcataacg cctgaaatgc tcactttgca
     1681 tcagcatggt gatacagctg atgtttattc taaaacctta ctcaagttct aagagagcac
     1741 ggattccctg tcattaataa tgatatccgt tagtgtagtc ttatgcattt caatgtactg
     1801 caatatttgc agatagagat cagaaagaat cacactggtt tcatctttta aacgcaagcg
     1861 taaagcctga tcaataatat gactatcaac atacccatgc gtcatgaaca cctccctctc
     1921 gagattttta aacaaagcac attgaaaaat aaatctctct gggatt
//
LOCUS       DAZKHF020000434         1954 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2201S31241
            SAMN43267800-rid25106413.denovo.437, whole genome shotgun sequence.
ACCESSION   DAZKHF020000434 DAZKHF020000000
VERSION     DAZKHF020000434.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267800
            Sequence Read Archive: SRR30307263
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1954)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1954)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 1954)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350581.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350581.2
            Genome Coverage       :: 151x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:21:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,652
            CDSs (total)                      :: 5,557
            Genes (coding)                    :: 5,320
            CDSs (with protein)               :: 5,320
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 237
            CDSs (without protein)            :: 237
            Pseudo Genes (ambiguous residues) :: 0 of 237
            Pseudo Genes (frameshifted)       :: 102 of 237
            Pseudo Genes (incomplete)         :: 130 of 237
            Pseudo Genes (internal stop)      :: 52 of 237
            Pseudo Genes (multiple problems)  :: 41 of 237
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1954
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267800-rid25106413.denovo.437"
                     /serotype="O 26:H11"
                     /isolate="2201S31241"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-01"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            complement(<1..>1954)
                     /locus_tag="ACHOSC_RS24345"
                     /old_locus_tag="pgaptmp_004869"
     CDS             complement(<1..>1954)
                     /locus_tag="ACHOSC_RS24345"
                     /old_locus_tag="pgaptmp_004869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1983 domain-containing protein"
                     /protein_id="HGU4550636.1"
                     /translation="TKMDAFGCTSRGQAHRAGLWLIKTELLETQTVDFSVGAEGLRHV
                     PGDVIEICDDDYAGISTGGRVLAVNSQTRTLTLDREITLPSSGTTLISLVDGSGNPVS
                     VEVQSVTDGVKVKVSRVPEGVAEYSVWGLKLPTLRQRLFRCVSIRENDDGTYAITAVQ
                     HVPEKEAIVDNGAHFDGEQSGTVNGVTPPAVQHLTAEVTADSGEYQVLARWDTPKVVK
                     GVSFLLRLTVTADDGSERLVSTARTTETTYRFTQLALGNYRLTVRAVNAWGQQGDAAS
                     VSFRIAAPAAPSRIELTPGYFQITATPHLAIYDPTVQFEFWFSEKRITDIRQVETTAR
                     YLGTALYWIAASINIKPGHDYYFYICSVNTVGKSAFVEAVGQPSDDASGYLDFFKGEI
                     GKTHLAQELWTQIDNGQLAPDLAEIRTSITDVSNEITQTVNKKLEDQSAAIQQIQKVQ
                     VDTNNNLNSMWAVKLQQMQDGRLYIAGIGAGIENTSDGMQSQVLLAADRIAMVNPANG
                     NTKPMFVGQGDQIFMNDVFLKRLTAPTITSGGNPPAFSLTPDGKLTAKNADISGSVNA
                     NAGTLNNVTINENCRVLGKLSANQIEGDLVKTVGKAFPRDSRAPERWPSGTITVRVYD
                     DQPFDRQIVIPAVAFSGAKHEREHTDI"
BASE COUNT          388 a          610 c          495 g          461 t
ORIGIN      
        1 aaatatcagt atgctctctc tcatgtttag cgccgctgaa tgccaccgcc ggaataacaa
       61 tctgccggtc aaacggctga tcgtcataaa ccctgacggt aatggtccct gatggccacc
      121 gttccggtgc acgggagtcc cgggggaaag ctttgcccac tgttttaacg agatcgcctt
      181 caatctggtt cgcggacagt tttcccagaa cccgacagtt ctcgttaatc gtgacattgt
      241 tgagcgtccc ggcgttcgca ttcacactgc cactgatatc cgcattttta gcggtcagct
      301 ttccgtccgg tgtcagggaa aatgccggtg gatttccacc gctggtaatg gtgggggccg
      361 tcaggcgttt caggaacacg tcgttcatga atatctgatc gccctgacca acaaacatcg
      421 gttttgtgtt gccattcgca ggattaacca tcgcaatcct gtccgccgcc agcagcacct
      481 gactctgcat gccgtcagag gtgttctcaa taccggcacc aatacccgcg atataaaggc
      541 gtccgtcctg catctgctgc agcttcacag cccacatgct gttcaggtta ttatttgtat
      601 caacctgaac cttctgtatc tgctgaattg ccgcactctg gtcttccagt ttcttattga
      661 cggtctgcgt gatttcattg ctgacatccg taatggacgt cctgatttca gccaggtcag
      721 gcgcaagctg accgttatca atctgcgtcc acaactcctg agccagatgg gttttcccta
      781 tctcgccttt gaaaaaatcc agatagccgg atgcatcatc actcggctgg ccgacggcct
      841 ccacaaatgc cgatttgcca acggtgttca cactgcagat gtaaaaataa taatcatggc
      901 ccggcttaat attgatactg gcggctatcc agtacagcgc cgtgccaaga tagcgggctg
      961 tggtttcaac ctgcctgata tcggtaatcc gcttttccga gaaccagaac tcaaactgta
     1021 ccgtcgggtc ataaatggca agatgcggcg tggcggttat ctgaaaatag cccggcgtca
     1081 gctcaatcct cgacggtgct gccggtgcgg caatccggaa cgataccgac gccgcatcgc
     1141 cctgctgccc ccacgcattt accgcccgga ctgtcagcct gtagttcccc agcgccagtt
     1201 gcgtgaagcg gtatgtggtt tccgtcgtcc gggccgtgct gaccagccgc tcactgccgt
     1261 cgtccgctgt tacggtcaga cggagcagga agctcacgcc cttcaccacc ttcggtgtgt
     1321 cccatcgcgc cagcacctga tattccccgc tgtctgcagt gacttctgcg gtcaggtgct
     1381 gcaccgctgg cggcgtgaca ccattcaccg tgccactctg ttcgccgtca aagtgcgccc
     1441 cgttatccac gatggcctct ttttccggca catgctgcac ggcggtgatg gcatacgtgc
     1501 cgtcgtcgtt ctcacggata ctcacgcagc ggaacaggcg ctggcgcagc gtcggcagct
     1561 tcagccccca tacgctgtat tcagcaacac cctcaggaac acggctcact tttaccttca
     1621 cgccgtcggt gacggactga acctccacgc tgaccggatt gccacttccg tcaaccaggc
     1681 ttatcagcgt ggtaccggag gatggcagcg tgatttcacg gtcgagcgtc agcgtccggg
     1741 tctggctgtt caccgccagc acacgaccac cggtgctgat accggcatag tcatcatcgc
     1801 agatttcaat aacatcgccc ggtacatggc gaagcccttc tgcgccgacg ctgaaatcca
     1861 cggtctgcgt ctccagcagc tccgttttaa tcagccacag cccggcgcgg tgtgcctgcc
     1921 cccggctggt acagccaaag gcatccatct tcgt
//
LOCUS       DAZKHF020000435         1947 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2201S31241
            SAMN43267800-rid25106413.denovo.438, whole genome shotgun sequence.
ACCESSION   DAZKHF020000435 DAZKHF020000000
VERSION     DAZKHF020000435.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267800
            Sequence Read Archive: SRR30307263
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1947)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1947)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 1947)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350581.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350581.2
            Genome Coverage       :: 151x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:21:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,652
            CDSs (total)                      :: 5,557
            Genes (coding)                    :: 5,320
            CDSs (with protein)               :: 5,320
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 237
            CDSs (without protein)            :: 237
            Pseudo Genes (ambiguous residues) :: 0 of 237
            Pseudo Genes (frameshifted)       :: 102 of 237
            Pseudo Genes (incomplete)         :: 130 of 237
            Pseudo Genes (internal stop)      :: 52 of 237
            Pseudo Genes (multiple problems)  :: 41 of 237
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1947
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267800-rid25106413.denovo.438"
                     /serotype="O 26:H11"
                     /isolate="2201S31241"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-01"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            43..>1947
                     /locus_tag="ACHOSC_RS24350"
                     /old_locus_tag="pgaptmp_004870"
     CDS             43..>1947
                     /locus_tag="ACHOSC_RS24350"
                     /old_locus_tag="pgaptmp_004870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6645 domain-containing protein"
                     /protein_id="HGU4550637.1"
                     /translation="MTFKHYDVVRAASPSDLAERLTQKLKEGWQPFGSPVAITPYTLM
                     QAIAAEGDVTTPVAVTGNEGKAVAVSATSDPEYYFVVVLAGQSNGMSYGEGLPLPETY
                     DRPDPRIKQLARRSTVTPGGAACKYNDIIPADHCLHDVQDMSRLNHPKADLSKGQYGT
                     VGQGLHIAKKLLPFIPANAGILLVPCCRGGSAFTTGADGTYSDASGASENSTRWGVDK
                     PLYKDLIGRTKAALKKNPKNVLFAVVWMQGEFDFDGTPGNHAAQFGALVDKFRADLTD
                     MAGQCVGGSAGGVPWICGDTTYFWKQKNESSYQTVYGSYKNKTEKNIHFVPFMTDENG
                     VNVPTNKPEEDPDIPGIGYYGSKWRDSSATWTSQDRASHFSSWARRGIISDRLATAIL
                     RHAGRVALNAGASSTVSEVRPSSSSGAEATGVTTLLSYLASESEGSLKVQGWSASGGR
                     AEVVSDAEGTGGKAVKLTKEAGKSSWVLEYAAGNGAALLQKGGQIRCRFKVSGALAAN
                     QYVMAFYWPVSSLPQGVALTGDGGNNLLAAFYIQTDAKDLNVMYHNAKVATNNLKLGT
                     FGAFDNEWHTLAFRFAGNNSLQVTPVIDGQDGTPFTLTQSPVSAFAADKLHVTDITRN
                     ATYPVLIDSIA"
BASE COUNT          463 a          471 c          613 g          400 t
ORIGIN      
        1 tgtgtattcc ggataattac tttgctggcg gcaggagagg ttatgacatt taaacactac
       61 gatgtggtca gggcggcatc gccgtcagac cttgctgaac gactgacaca aaaactgaag
      121 gagggctggc agccatttgg cagtccggtg gccatcacgc cttataccct gatgcaggcc
      181 attgcggcgg aaggtgatgt caccacacca gtggcggtga ccggtaatga gggtaaggcg
      241 gtggctgtca gtgccaccag cgacccggag tattactttg ttgtggttct ggcagggcag
      301 tcaaatggca tgtcgtatgg tgaaggtctt ccgctgccgg agacatatga ccgtccggac
      361 ccgcgtatta agcagctggc gcgccgcagt acggtgacac cgggcggtgc agcatgcaaa
      421 tataacgaca tcattccggc ggaccattgt ctgcatgatg tgcaggacat gagccgtctt
      481 aaccatccga aagcggacct gtcaaaaggg cagtacggta ccgtggggca ggggctgcat
      541 atcgccaaaa aactgctgcc gtttataccg gcgaatgcgg gcattctgct ggttccgtgc
      601 tgtcgtggtg gttcagcgtt caccaccgga gctgatggca catacagtga cgcgagtggt
      661 gcctcggaga attcaacccg ctggggtgtg gacaagccgc tgtataagga ccttatcggt
      721 cgaacaaaag cagcactgaa gaagaacccg aaaaatgtgc tgtttgccgt ggtgtggatg
      781 cagggggaat ttgattttga cggcacgccc ggaaatcacg cagcacagtt tggtgcgctg
      841 gttgataaat tccgtgcaga cctgacggat atggcaggtc agtgcgttgg tggctctgct
      901 ggcggtgttc cctggatctg tggagatacg acgtatttct ggaagcagaa gaacgaatcc
      961 tcgtaccaga cggtgtacgg cagctacaaa aacaaaacgg aaaagaatat ccatttcgta
     1021 ccgttcatga ccgatgagaa cggggtgaat gtgccgacga acaaaccgga agaagacccg
     1081 gacattccgg gtatcgggta ttacggttcg aaatggcgtg acagctcagc cacctggacg
     1141 tcacaggaca gggcgagcca tttcagttca tgggctcgcc gcgggattat ttccgaccgt
     1201 ctggcaacgg cgattttgcg ccatgcggga agagtggcgc taaacgcggg ggcatcatcg
     1261 acagtatcag aggtgcgccc gtcatcgtct tccggtgcag aagccacagg cgtcacaaca
     1321 ctgctctctt accttgccag cgagtcagag ggaagcctga aagtacaggg atggtcagcc
     1381 agtggcggca gggcagaagt ggtcagcgat gcggagggaa ccggaggtaa ggcagtgaag
     1441 ctgaccaagg aagcaggtaa aagcagctgg gtgctggagt acgccgcggg caacggtgcg
     1501 gctctgttac agaaaggggg gcagattcgc tgccgcttta aggtttcggg agcgctggct
     1561 gcgaaccagt atgttatggc gttttactgg ccggtctctt cactgccaca gggcgttgcc
     1621 ctgaccggag acggggggaa taacctgctg gcagcgttct acatccagac agatgcaaaa
     1681 gacctgaatg tgatgtacca caatgcgaaa gtggcgacaa acaacctgaa actgggaacc
     1741 tttggcgcat ttgataacga atggcatacg ctggctttcc gctttgccgg gaataacagc
     1801 cttcaggtga cgccggttat tgatggtcag gatggcacac cgttcacgct gacgcagtca
     1861 ccggtcagtg catttgcggc ggataaactg catgtgacag acattaccag gaatgcgact
     1921 tacccggtac tgatagacag cattgcg
//
LOCUS       DAZKHF020000436         1941 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2201S31241
            SAMN43267800-rid25106413.denovo.439, whole genome shotgun sequence.
ACCESSION   DAZKHF020000436 DAZKHF020000000
VERSION     DAZKHF020000436.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267800
            Sequence Read Archive: SRR30307263
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1941)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1941)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 1941)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350581.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350581.2
            Genome Coverage       :: 151x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:21:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,652
            CDSs (total)                      :: 5,557
            Genes (coding)                    :: 5,320
            CDSs (with protein)               :: 5,320
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 237
            CDSs (without protein)            :: 237
            Pseudo Genes (ambiguous residues) :: 0 of 237
            Pseudo Genes (frameshifted)       :: 102 of 237
            Pseudo Genes (incomplete)         :: 130 of 237
            Pseudo Genes (internal stop)      :: 52 of 237
            Pseudo Genes (multiple problems)  :: 41 of 237
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1941
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267800-rid25106413.denovo.439"
                     /serotype="O 26:H11"
                     /isolate="2201S31241"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-01"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            1..202
                     /locus_tag="ACHOSC_RS24355"
                     /old_locus_tag="pgaptmp_004871"
                     /pseudo
     CDS             1..202
                     /locus_tag="ACHOSC_RS24355"
                     /old_locus_tag="pgaptmp_004871"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000807328.1"
                     /note="internal stop; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
     gene            331..576
                     /locus_tag="ACHOSC_RS24360"
                     /old_locus_tag="pgaptmp_004872"
     CDS             331..576
                     /locus_tag="ACHOSC_RS24360"
                     /old_locus_tag="pgaptmp_004872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000344551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4550638.1"
                     /translation="MDIRHSLNEWWTLWQASRAAIELDIDWPESNDDFWKDGEEGAYA
                     MGYEDGRDKTVIAVMKAIRAAGIKEKNFDEANNIPPN"
     gene            548..958
                     /locus_tag="ACHOSC_RS24365"
                     /old_locus_tag="pgaptmp_004873"
     CDS             548..958
                     /locus_tag="ACHOSC_RS24365"
                     /old_locus_tag="pgaptmp_004873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015966857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination protein NinB"
                     /protein_id="HGU4550639.1"
                     /translation="MKQTIFLRTKQQQQAAINAILATPLDKDKPVTIRITDYKRNLDQ
                     NAKFHAMLADIARQVQWCDKWLKPEQWKVLLISGHAVATKQEADVLPGLEGEYVNIRE
                     SSAQMSVKRMASLIEYTTAWAIGQGVRFTDRRYE"
     gene            955..1131
                     /locus_tag="ACHOSC_RS24370"
                     /old_locus_tag="pgaptmp_004874"
     CDS             955..1131
                     /locus_tag="ACHOSC_RS24370"
                     /old_locus_tag="pgaptmp_004874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NinE family protein"
                     /protein_id="HGU4550640.1"
                     /translation="MRRQRRSITDIICENCKYLPTKRSRNKRKPIPKESDVKTFNYTA
                     HLWDIRWLRHRARK"
     gene            1134..1535
                     /locus_tag="ACHOSC_RS24375"
                     /old_locus_tag="pgaptmp_004875"
     CDS             1134..1535
                     /locus_tag="ACHOSC_RS24375"
                     /old_locus_tag="pgaptmp_004875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4550641.1"
                     /translation="MLLIQPGFGLSIKKGHMFGEKESQRKMVSIRLPFISIYWLNREA
                     TNYWYTCARAAFNDPDWFVKNHHAVRQAKRKANMTYMKAYQKAWKEHRDRYQQDMEKL
                     ESENMELRRKLGEAKRDIDAYKRLFNGESHA"
     gene            1495..1704
                     /locus_tag="ACHOSC_RS24380"
                     /old_locus_tag="pgaptmp_004876"
     CDS             1495..1704
                     /locus_tag="ACHOSC_RS24380"
                     /old_locus_tag="pgaptmp_004876"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001519596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein NinF"
                     /protein_id="HGU4550642.1"
                     /translation="MLTSDFLMVKAMLSPSQSLQYQKESVERALTCANCGQKLHVLEV
                     HVCEYCCAELMSDPNSSMYEEEDDE"
     gene            1697..>1941
                     /locus_tag="ACHOSC_RS24385"
                     /old_locus_tag="pgaptmp_004877"
     CDS             1697..>1941
                     /locus_tag="ACHOSC_RS24385"
                     /old_locus_tag="pgaptmp_004877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001004257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rha family transcriptional regulator"
                     /protein_id="HGU4550643.1"
                     /translation="MNELINGNAIKMTSIEIAELVGKRHDNVKRTIETLAKNGVIRLP
                     QIEVSERINNLGFNVQYEHYVFEGEQGKRDSIVVVAQ"
BASE COUNT          612 a          386 c          490 g          453 t
ORIGIN      
        1 tgagtttatg ctcgtcgcac tcaaatgcgt tggcgtttga tggattcttc tgacgttatt
       61 tattgttctg catagctaca ttcgtcttgt gaatgacggt aaagacccat ggtatacgtt
      121 gtttggcgct gcatttgtct gggtgattat cggtgttgcg cctgtcgctg tagcaaaaat
      181 ggcgtggcgt tttgtgagtt gaactgaggg taagtatcga tggacgaatc aagaaagcag
      241 tttgaggaat acgttgccaa aaaattgaga ttaccattcg agatgataac cgaggcaaga
      301 aatggtgata ggtacttcgc attttcaagc atggatattc gtcactcctt aaatgagtgg
      361 tggactttat ggcaggcatc gcgagcagct attgaactgg atatcgactg gccagaatcg
      421 aatgacgact tttggaaaga tggtgaagaa ggtgcttatg cgatgggtta tgaggatggg
      481 cgtgacaaaa cggtaattgc agtaatgaaa gctatcagag ccgctggaat taaagagaag
      541 aatttcgatg aagcaaacaa tattcctccg aactaagcaa caacagcaag ccgcaatcaa
      601 cgccatcctc gcaacaccac tcgataaaga caagccagtc accatccgca ttactgacta
      661 caagcgcaac cttgaccaga acgcaaaatt tcacgcgatg ctggcggata tcgcacgtca
      721 ggttcaatgg tgcgataagt ggttaaaacc agaacaatgg aaggttttgt tgattagcgg
      781 tcatgcagtg gcaacaaagc aggaagctga tgttttgccc ggccttgaag gcgaatacgt
      841 caacattcgc gaaagtagcg cgcagatgag tgtgaagcgt atggcaagtc tgattgagta
      901 cacgacagca tgggctattg gtcagggcgt cagatttacc gacaggaggt acgaatgaga
      961 cgacagcgac gaagtatcac cgacataatc tgtgaaaact gcaaatacct tccaacgaaa
     1021 cgctccagaa ataaacgcaa gccaatccca aaagaatctg acgtaaaaac cttcaactac
     1081 acggctcacc tgtgggatat ccggtggcta agacatcgtg cgaggaaatg acaatgcttt
     1141 taattcaacc tggatttgga cttagcatca aaaaaggcca catgtttggc gagaaagagt
     1201 cacaacgaaa aatggtgtct atccggttgc catttatcag tatttattgg ctaaacaggg
     1261 aggcaacaaa ttattggtat acatgcgcca gagcagcatt taacgaccct gactggtttg
     1321 tgaaaaacca ccacgcagtt cgtcaggcaa agagaaaggc caatatgaca tacatgaagg
     1381 cgtatcaaaa agcatggaaa gaacaccgcg atcgatacca acaagacatg gaaaagcttg
     1441 aatcagaaaa catggaatta agacgaaagc tcggtgaagc aaaacgagac attgatgctt
     1501 acaagcgact ttttaatggt gaaagccatg cttagcccat cccaatccct tcaataccag
     1561 aaagaaagcg tcgagcgagc tttaacgtgc gctaactgcg gtcagaagct gcatgtgctg
     1621 gaagttcacg tgtgtgagta ctgctgcgca gaactgatga gcgatccgaa tagctcaatg
     1681 tacgaggaag aagacgatga atgagttaat aaatggcaat gccatcaaaa tgacaagcat
     1741 tgaaatagct gagttggtgg gtaagcgtca tgacaatgtg aaacgtacca tcgaaacgct
     1801 ggctaaaaat ggtgttatcc ggcttcctca aattgaggtt tccgaaagaa tcaataactt
     1861 agggttcaat gttcagtacg agcattacgt cttcgagggc gaacaaggta agcgagacag
     1921 tattgtcgtt gttgcccagt t
//
LOCUS       DAZKHF020000437         1928 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2201S31241
            SAMN43267800-rid25106413.denovo.440, whole genome shotgun sequence.
ACCESSION   DAZKHF020000437 DAZKHF020000000
VERSION     DAZKHF020000437.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267800
            Sequence Read Archive: SRR30307263
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1928)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1928)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 1928)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350581.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350581.2
            Genome Coverage       :: 151x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:21:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,652
            CDSs (total)                      :: 5,557
            Genes (coding)                    :: 5,320
            CDSs (with protein)               :: 5,320
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 237
            CDSs (without protein)            :: 237
            Pseudo Genes (ambiguous residues) :: 0 of 237
            Pseudo Genes (frameshifted)       :: 102 of 237
            Pseudo Genes (incomplete)         :: 130 of 237
            Pseudo Genes (internal stop)      :: 52 of 237
            Pseudo Genes (multiple problems)  :: 41 of 237
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1928
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267800-rid25106413.denovo.440"
                     /serotype="O 26:H11"
                     /isolate="2201S31241"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-01"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            <1..552
                     /locus_tag="ACHOSC_RS24390"
                     /old_locus_tag="pgaptmp_004878"
     CDS             <1..552
                     /locus_tag="ACHOSC_RS24390"
                     /old_locus_tag="pgaptmp_004878"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030430 - host cell cytoplasm [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0046718 - symbiont entry into host cell
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage minor tail protein L"
                     /protein_id="HGU4550644.1"
                     /translation="TWQGREYQAYPIEGSGFEMNGKGSSARPSLTVSNLFGLVTGMAE
                     DLQSLVGATVVRRRVYARFLDAVNFVAGNPEADPEQELTDRWVVEQMSSLTAMTASFV
                     LATPTETDGALFPGRIMLANTCMWDYRSDECGYNGPAVADEFDNPTTDIRKDRCSKCM
                     RGCELRRNVGNFGGFLSINKLSQ"
     gene            563..1306
                     /locus_tag="ACHOSC_RS24395"
                     /old_locus_tag="pgaptmp_004879"
     CDS             563..1306
                     /locus_tag="ACHOSC_RS24395"
                     /old_locus_tag="pgaptmp_004879"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310264.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C40 family peptidase"
                     /protein_id="HGU4550645.1"
                     /translation="MTQTESAILAHARRCAPAESCGFVISTPEGEWYIPCVNISAEPE
                     AYFRIAPEDWLQAEMQGEIVALVHSHPGGLPWLSEADRRLQIKSALPWWLVSRGDIHK
                     FRCVPHLTGRRFEHGVTDCYTLFRDAYHLAGTEMPDFHREDDWWRNGQNLYLDNMEGT
                     GFYRVPLSSAQAGDILLCCFGASVPNHAAIYCGNGELLHHLPEQLSKRERYSEKWQRR
                     THSVWRHRHWHASAFTGIYNDLAAASACM"
     gene            1303..1884
                     /locus_tag="ACHOSC_RS24400"
                     /old_locus_tag="pgaptmp_004880"
     CDS             1303..1884
                     /locus_tag="ACHOSC_RS24400"
                     /old_locus_tag="pgaptmp_004880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310014.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tail assembly protein"
                     /protein_id="HGU4550646.1"
                     /translation="MNTAAEAIRALSLQVPGFRRQMNEGWYQIRIAGDDTAPEAVYAR
                     LHEQLGEGTVIHIVPRLAGAGKGGLQIVLGAAAIVGSFFTAGASMAAWGAALSAGGFS
                     ATTMLFSLGASMILGGVAQMLAPKPKTPEYRATDNGKQNTYFSSLDNMIAQGNPMPVP
                     YGEMLVGSRRISQDISTRDEGGGGKVVVIGRQA"
BASE COUNT          388 a          499 c          644 g          397 t
ORIGIN      
        1 acctggcagg ggcgtgaata tcaggcgtac ccgattgagg ggagtggctt tgagatgaac
       61 ggaaagggca gcagtgcccg cccgtcgctg acagtgtcca atcttttcgg ccttgtcacc
      121 gggatggcgg aggatttgca gagcctggtg ggtgccacgg tggtccgtcg ccgggtgtat
      181 gcgcgttttc tggatgcggt gaattttgtg gcaggcaatc ctgaggcaga cccggagcag
      241 gagctgacgg accggtgggt ggtggagcag atgtcatcgc tgacggccat gacggcctcg
      301 tttgtgctgg cgacaccgac ggagacggac ggtgcgctgt ttcccggtcg catcatgctg
      361 gcgaacacct gtatgtggga ttaccgctct gatgaatgcg ggtataacgg tccggcagtg
      421 gcggatgagt tcgacaaccc caccacggat atccggaagg acagatgcag taaatgcatg
      481 cgcgggtgtg aactgcgcag gaatgtcggc aactttggcg gcttcctttc cattaataaa
      541 ctttcgcagt aaatcccggt ttatgacaca gactgaatca gcgattctgg cgcatgcccg
      601 gcggtgtgcg cctgcggagt cgtgcggctt cgtgataagc acgccggagg gggagtggta
      661 tatcccttgt gtgaatattt ccgcagagcc ggaggcgtat tttcgtatcg caccggaaga
      721 ctggctgcag gcagagatgc agggggagat tgtggcgctg gttcacagtc atcccggtgg
      781 tctgccctgg ctgagcgagg ctgaccggcg gctgcagata aaaagcgcac tgccctggtg
      841 gctggtcagc cggggtgaca ttcacaaatt ccgctgcgtt ccgcacctga ccggacggcg
      901 ctttgaacac ggggtgacgg actgttacac cctgttccgg gatgcgtacc atctggcggg
      961 gactgaaatg ccggattttc atcgcgagga tgactggtgg cgcaacggcc agaacctgta
     1021 cctggacaat atggagggaa cgggctttta ccgggtgccc ctgtcctctg cacaggcggg
     1081 cgatattctg ctgtgctgct ttggtgcttc ggtaccgaac catgccgcca tttactgtgg
     1141 caacggtgag ctgcttcacc atctgcctga acaactgagt aaacgggaga ggtattccga
     1201 aaaatggcaa cgacgaacgc attctgtctg gcgtcaccgc cactggcacg catctgcctt
     1261 cacggggatt tacaacgatt tggccgccgc ctcagcctgt atgtgaacac ggcagcggaa
     1321 gccatccggg cgctgtcgtt acaggtgccc ggattccgcc gtcagatgaa cgaaggctgg
     1381 taccagatac gtattgccgg tgatgacacg gcaccggagg cggtgtacgc ccgtcttcac
     1441 gaacagctgg gtgagggaac ggtcatccac attgtgccgc gactggccgg ggccggaaag
     1501 ggtggactgc agattgtgct gggggcggca gccatcgtgg gctctttctt cacggccgga
     1561 gcatcgatgg cagcctgggg tgcagcgctg agtgccggcg gtttttctgc caccacgatg
     1621 ctgttttcac tgggtgccag catgatactg ggtggtgtgg cacagatgct ggccccgaag
     1681 ccaaaaacac cggaatacag ggcaacggat aacggtaaac agaacacgta cttttcgtcg
     1741 ctggacaaca tgattgccca gggtaacccg atgccggtgc cttacgggga aatgctggtt
     1801 ggctcccgcc gtatatccca ggacatcagt acccgtgatg aaggcggggg cggaaaggtc
     1861 gtggttatcg ggcggcaggc ataaaagcga aaaaatcccg cagtgctcac ggacaggaac
     1921 tgcgggag
//
LOCUS       DAZKHF020000438         1922 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2201S31241
            SAMN43267800-rid25106413.denovo.441, whole genome shotgun sequence.
ACCESSION   DAZKHF020000438 DAZKHF020000000
VERSION     DAZKHF020000438.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267800
            Sequence Read Archive: SRR30307263
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1922)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1922)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 1922)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350581.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350581.2
            Genome Coverage       :: 151x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:21:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,652
            CDSs (total)                      :: 5,557
            Genes (coding)                    :: 5,320
            CDSs (with protein)               :: 5,320
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 237
            CDSs (without protein)            :: 237
            Pseudo Genes (ambiguous residues) :: 0 of 237
            Pseudo Genes (frameshifted)       :: 102 of 237
            Pseudo Genes (incomplete)         :: 130 of 237
            Pseudo Genes (internal stop)      :: 52 of 237
            Pseudo Genes (multiple problems)  :: 41 of 237
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1922
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267800-rid25106413.denovo.441"
                     /serotype="O 26:H11"
                     /isolate="2201S31241"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-01"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            complement(<1..752)
                     /locus_tag="ACHOSC_RS24405"
                     /old_locus_tag="pgaptmp_004881"
     CDS             complement(<1..752)
                     /locus_tag="ACHOSC_RS24405"
                     /old_locus_tag="pgaptmp_004881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415949.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-guided endonuclease InsQ/TnpB family
                     protein"
                     /protein_id="HGU4550647.1"
                     /translation="MKRLQAFKFQLRPGGQQECEMRRFAGACRFVFNRALARQNENHE
                     AGNKYIPYGKMASWLVEWKNATETQWLKDSPSQPLQQSLKDLERAYKNFFRKRAAFPR
                     FKKRGQNDAFRYPQGVKLDQENSRIFLPKLGWMRYRNSRQVTGVVKNVTVSQSCGKWY
                     ISIQTESEVSTPAHPSASMVGLDAGVAKLATLSDGTVFEPVNSFQKNQKTLARLQRQL
                     SRKVKFSNNWQKQKRKIQRLHSCIANIRRDYL"
     gene            760..1261
                     /gene="tnpA"
                     /locus_tag="ACHOSC_RS24410"
                     /old_locus_tag="pgaptmp_004882"
                     /pseudo
     CDS             760..1261
                     /gene="tnpA"
                     /locus_tag="ACHOSC_RS24410"
                     /old_locus_tag="pgaptmp_004882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313209.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605 family transposase"
     gene            complement(1279..>1922)
                     /locus_tag="ACHOSC_RS24415"
                     /old_locus_tag="pgaptmp_004883"
     CDS             complement(1279..>1922)
                     /locus_tag="ACHOSC_RS24415"
                     /old_locus_tag="pgaptmp_004883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF3313 domain-containing protein"
                     /protein_id="HGU4550648.1"
                     /translation="AALCGLLALSGCASKITQPDKYSGFLNNYSDLKETTSATGKPVL
                     RWVDPSFDQSKYDSIVWNPITYYPVPKPSTQVGQKVLDKILNYTNTEMKEAIAQRKPL
                     VTTAGPRSLIFRGAITGVDTSKEGLQFYEVVPVALVVAGTQMATGHRTMDTRLYFEGE
                     LIDAATNKPVIKVVRQGEGKDLNNESTPMAFENIKQVIDDMATDATMFDVNKK"
BASE COUNT          476 a          462 c          446 g          538 t
ORIGIN      
        1 tgaaggtagt ccctgcggat atttgcgata caggaatgca gtcgctgtat tttgcgtttc
       61 tgcttctgcc agttgttgct gaatttgacc ttgcggctta actgtcgctg aagtctcgcc
      121 agcgtcttct ggtttttctg aaaactgttt acaggctcaa agactgtgcc atctgacagc
      181 gtggcgagtt tagccacgcc agcatccagc ccgaccattg atgctgaagg gtgagccgga
      241 gtggatactt cactttctgt ctgaatactg atgtaccact taccgcagga ctggctgaca
      301 gtgacatttt tcacaacacc cgtgacctga cggctattcc ggtagcgcat ccagcccagt
      361 ttcggcagaa aaatacggct gttttcctga tcgagcttaa cgccctgcgg gtagcggaat
      421 gcatcattct gtccccgctt tttgaatcgg ggaaaagcag cccgcttccg gaagaagttt
      481 ttgtaagccc gctcaaggtc tttcagtgac tgttgcaatg gctgtgaggg agaatcttta
      541 agccattgcg tttcagtggc atttttccac tcaaccagcc aggaagccat tttcccgtaa
      601 gggatgtatt tattaccggc ctcatgattc tcattctgac gtgccagagc acgattgaaa
      661 acgaaacgac atgcgcctgc gaagcgcctc atttcacact cctgttgacc acctggtctt
      721 aactggaatt taaatgcctg taatcgtttc acaggtcaaa tgatactttg gtctgtgaat
      781 aaagaaaccg atatccggcg tggaaggcat tgtgttttcc tgatgcatgt tcacctggtc
      841 tttgtcacca gataccgacg ccagattttt tgacgcgaca gaaaaactac gcacttactt
      901 ttcaaatgta tgtgcttatt ttgaagctga actggttgaa atggatggcg aaccagatca
      961 cgtccatttg ttaattaact aacctcccaa actggcgata tccagtctgg taaacagcct
     1021 caaaggcgta tcgggtaggt tactgcgacg agatcgacca gatattgcag tcaggtatta
     1081 ctacaaaggc gttttgtgga gtcctggcta ttttgccagt agctgcggag gtgcgccaat
     1141 atccgtcatc cgccaataca ttgaacaaca gcaaacacct ggtcaggtgg aaaaccgcac
     1201 cttatatccc cgccctgaag gatggggttt tacggcacac cggataaaaa gcgccatcta
     1261 acgatggcgc gtctggaact actttttgtt aacgtcaaac atggtggcat cggtcgccat
     1321 gtcatcaata acttgtttaa tattttcgaa agccattggc gtactttcgt tattcaggtc
     1381 tttaccttcg ccctgacgaa cgactttgat aaccggttta ttagtcgctg catcaatcag
     1441 ctcaccttca aaatagaggc gagtatccat ggtacggtgg cctgtagcca tttgcgtccc
     1501 cgcaaccact aatgcaacag gaaccacttc atagaattgc agcccttctt tgctggtatc
     1561 tacaccggta atggccccac ggaaaatcag actacgcggc ccagcggtgg taaccagtgg
     1621 tttacgctgc gctatcgctt ctttcatttc ggtgttggta tagttcaaaa ttttatccag
     1681 aactttctgc cctacctggg tagaaggttt tggcaccgga taataagtga ttgggttcca
     1741 gacgatgctg tcatatttgc tttgatcaaa actcgggtct acccaacgta aaacaggttt
     1801 acctgttgct gaggttgttt cttttaaatc agagtaattg tttaaaaaac cagaatattt
     1861 atctggctgg gtgattttag aggcacaacc agacagagcc aacaagccgc ataaagctgc
     1921 aa
//
LOCUS       DAZKHF020000439         1917 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2201S31241
            SAMN43267800-rid25106413.denovo.442, whole genome shotgun sequence.
ACCESSION   DAZKHF020000439 DAZKHF020000000
VERSION     DAZKHF020000439.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267800
            Sequence Read Archive: SRR30307263
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1917)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1917)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 1917)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350581.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350581.2
            Genome Coverage       :: 151x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:21:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,652
            CDSs (total)                      :: 5,557
            Genes (coding)                    :: 5,320
            CDSs (with protein)               :: 5,320
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 237
            CDSs (without protein)            :: 237
            Pseudo Genes (ambiguous residues) :: 0 of 237
            Pseudo Genes (frameshifted)       :: 102 of 237
            Pseudo Genes (incomplete)         :: 130 of 237
            Pseudo Genes (internal stop)      :: 52 of 237
            Pseudo Genes (multiple problems)  :: 41 of 237
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1917
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267800-rid25106413.denovo.442"
                     /serotype="O 26:H11"
                     /isolate="2201S31241"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-01"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            578..1861
                     /gene="ydfJ"
                     /locus_tag="ACHOSC_RS24420"
                     /old_locus_tag="pgaptmp_004884"
                     /pseudo
     CDS             578..1861
                     /gene="ydfJ"
                     /locus_tag="ACHOSC_RS24420"
                     /old_locus_tag="pgaptmp_004884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000582800.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MHS family MFS transporter YdfJ"
BASE COUNT          515 a          371 c          471 g          560 t
ORIGIN      
        1 tatatgatat agttgacaat ttttatcttg ggtgttctta aagttcgtag ataaacattg
       61 tcgtttcagg tatacagaaa tgctaacagg tggcagcaaa aatcaggcgg tttatggcgc
      121 aagctgaagc ggcaactgca aactatctta tgcagagact ctacacggat tgggtttaaa
      181 agtatacata gataacagtt tttatctgaa aaagaaaaat atcaaggtga tatagcctat
      241 atgcctttga tgtggaggaa tgaatgtgat gggagtgatg tatctgaata gttgaaaaac
      301 cgcagacaca ccttatgcaa gaacgtgctg cggttggctg gtaaattttt cgatagtgcg
      361 agtattgaat gatttccagc cgttattgat tttacgcgta aatccatgaa aaaactactt
      421 atctgttggg gagttttttg gggcatatat gggacagaaa taggccccag atagacactg
      481 agatcgaact taggatgctt ttaaaaaaat gcaactatct gaaaaaacct agaaaacgcc
      541 aaggaaacca caggatggga aaaaacacct gtgaattatg gatttccagt tatattcgct
      601 cggcgcagcg ttagtgtttc atgaaatatt ttttcctgaa tcatcaacgg caatggcgtt
      661 aattttggca atgggaacct acggtgcagg ttatgtggcg cgtattgtcg gagcatttat
      721 tttcggcaaa atgggcgaca gaatagggcg taaaaaagtg ctctttatta ccatcaccat
      781 gatggggatc tgtaccacct taattggtgt gttaccgacc tatgcacaga ttggtgtttt
      841 tgcccccatc ttgctggtga cgctgcgtat tattcagggg ttgggtgcag gtgcggaaat
      901 ttccggtgcc ggtacgatgc tggcggaata tgcgccaaaa ggtaagcgcg gaattatctc
      961 ctcatttgta gctatgggaa ctaactgcgg aaccttgagc gcaacggcaa tctgggcctt
     1021 tatgttcttc attctcagta aagaggaact gcttgcgtgg ggatggcgta taccgttcct
     1081 ggcgagcgtt gtcgtgatgg tctttgctat ctggttgcgt atgaatctga aagaaagccc
     1141 ggtctttgag aaggttaacg acagcaacca acccacagca aaacctgcac ctgctggtag
     1201 catgttccag agcaaatcct tctggctggc aacagggctg cgttttggtc aggcgggtaa
     1261 ctccgggtta attcagactt tccttgcagg ctatttagtg cagacgttat tgtttaacaa
     1321 agcaattcca acagatgcat tgatgattag ttcgattctc ggctttatga ccattccgtt
     1381 ccttggttgg ttatccgata aaattggtcg ccggatcccg tatattatta tgaatacctc
     1441 cgcgattgtg ctggcatggc caatgctttc tatcattgta gataaaagct atgccccgag
     1501 caccattatg gttgcactga ttgtgattca taactgtgcg gtgctgggat tatttgctct
     1561 ggaaaacatt accatggcag aaatgttcgg ctgtaaaaac cgctttaccc ggatggctat
     1621 ttctaaagaa attggtggtc ttatcgcttc cggttttggt cctatcctgg cgggtatttt
     1681 ctgcaccatg acggaatcct ggtatccgat cgccattatg atcatggcat attcagtaat
     1741 tggtttaatc tctgcgctga aaatgccaga ggtgaaagac cgtgatttaa gtgcgctgga
     1801 agacgctgcg gaagatcaac cgcgtgttgt aagagctgcg caaccttcca gaagtctgta
     1861 aatcctcaat cccttctctt accggagagg ggatttttat tcatataaca aaacata
//
LOCUS       DAZKHF020000440         1896 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2201S31241
            SAMN43267800-rid25106413.denovo.443, whole genome shotgun sequence.
ACCESSION   DAZKHF020000440 DAZKHF020000000
VERSION     DAZKHF020000440.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267800
            Sequence Read Archive: SRR30307263
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1896)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1896)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 1896)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350581.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350581.2
            Genome Coverage       :: 151x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:21:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,652
            CDSs (total)                      :: 5,557
            Genes (coding)                    :: 5,320
            CDSs (with protein)               :: 5,320
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 237
            CDSs (without protein)            :: 237
            Pseudo Genes (ambiguous residues) :: 0 of 237
            Pseudo Genes (frameshifted)       :: 102 of 237
            Pseudo Genes (incomplete)         :: 130 of 237
            Pseudo Genes (internal stop)      :: 52 of 237
            Pseudo Genes (multiple problems)  :: 41 of 237
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1896
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267800-rid25106413.denovo.443"
                     /serotype="O 26:H11"
                     /isolate="2201S31241"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-01"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            complement(372..448)
                     /locus_tag="ACHOSC_RS24425"
                     /old_locus_tag="pgaptmp_004885"
     tRNA            complement(372..448)
                     /locus_tag="ACHOSC_RS24425"
                     /old_locus_tag="pgaptmp_004885"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(412..414),aa:Arg,seq:tct)
     gene            complement(462..538)
                     /locus_tag="ACHOSC_RS24430"
                     /old_locus_tag="pgaptmp_004886"
     tRNA            complement(462..538)
                     /locus_tag="ACHOSC_RS24430"
                     /old_locus_tag="pgaptmp_004886"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(502..504),aa:Arg,seq:tcg)
     gene            complement(548..623)
                     /locus_tag="ACHOSC_RS24435"
                     /old_locus_tag="pgaptmp_004887"
     tRNA            complement(548..623)
                     /locus_tag="ACHOSC_RS24435"
                     /old_locus_tag="pgaptmp_004887"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(588..590),aa:Ile,seq:cat)
     gene            complement(664..777)
                     /locus_tag="ACHOSC_RS24440"
                     /old_locus_tag="pgaptmp_004888"
                     /pseudo
     CDS             complement(664..777)
                     /locus_tag="ACHOSC_RS24440"
                     /old_locus_tag="pgaptmp_004888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309807.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA methylase"
     gene            complement(1065..1499)
                     /locus_tag="ACHOSC_RS24445"
                     /old_locus_tag="pgaptmp_004889"
     CDS             complement(1065..1499)
                     /locus_tag="ACHOSC_RS24445"
                     /old_locus_tag="pgaptmp_004889"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0060567 - negative regulation of
                     termination of DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="antiterminator Q family protein"
                     /protein_id="HGU4550649.1"
                     /translation="MRDIRQVLERWGAWAANNHEDVTWSPIAAGFKGLIPEKVKSRPQ
                     CCDDDAMIICGCMARLNRNNSDLHDLLVDYYVLGETFMALARKHGCSDTCIGKRLHKA
                     EGIVEGMLMMLGVRLEMDRYVERELPGGRTSVFYQRKNSLRS"
     gene            complement(1492..1686)
                     /locus_tag="ACHOSC_RS24450"
                     /old_locus_tag="pgaptmp_004890"
     CDS             complement(1492..1686)
                     /locus_tag="ACHOSC_RS24450"
                     /old_locus_tag="pgaptmp_004890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage NinH family protein"
                     /protein_id="HGU4550650.1"
                     /translation="MTFTVKTIPDMLLEAYGNQSEVARILNCNRATVRKYIGDKEGKK
                     HAVVNGVLMVHRGWGKDTDA"
     gene            complement(1683..>1896)
                     /locus_tag="ACHOSC_RS24455"
                     /old_locus_tag="pgaptmp_004891"
     CDS             complement(1683..>1896)
                     /locus_tag="ACHOSC_RS24455"
                     /old_locus_tag="pgaptmp_004891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="recombination protein NinG"
                     /protein_id="HGU4550651.1"
                     /translation="HKQCVVCNQHKSGNLVPYRVELINRIGQEAVDEIESNHNRHRWT
                     IEECKAIKAGYQQKLKDLRNSRSEAA"
BASE COUNT          463 a          505 c          421 g          507 t
ORIGIN      
        1 aatgacaatg caagtatctc atgctgttgc ccgaacctac tcgggctttt tttgcatgta
       61 aaaaggctcc tgcgatgagg ggcctggata tatgcctaat ctctgtatac agcatgatgc
      121 cgggtgcctc ccggtgaatt ctgcaatgac cagacagaat ccgcaacttg cctatacaat
      181 acgcaaccaa acatctgtca ttatgccccg ccgcccaggg ggattcatca tgcaggattt
      241 ttttaacaaa cgctcagcat gtcaggcaac agtcgactac ctgaattgtg aggcatttaa
      301 catttcactg tccggtgtct ttcctgtaat aaaaagcccg caaaagagag tcagggcaga
      361 taagtgtggt gtggcgcgtt gtactggatt cgaaccagtg accgattgct tagaaggcaa
      421 ttgctctgtc cggctgagct aacaacgcat gatgctgata atggaccgcc atcggggact
      481 tgaaccccgc acagccagct tcgaaggctg acgctctatc ccgatgagct aatggcggta
      541 tgtgatatgg tggcccttgc tggatttgaa ccagcgacct ggcgattatg agtcgctcgc
      601 tctcaccact gagctaaagg gccgggcgca ggataataac ggtacgtaac taatcctgca
      661 atatcatccg ttctgactga ctaaatcctg aacttccctg accgtctgct caaaacgttc
      721 agtctccagc tcaacgccaa ttgcacgacg ccccagcgac attgctgctt tgacgctaca
      781 gacataaaaa agccagccac tgggggaggc tggcaaactc gtagagcaaa atgctgttac
      841 gcaaacttcg ttacagggtc atcctgcaat acaaaaaata cacaatattt agaaaactaa
      901 tagtgccatg tgcaattttt aagattttgt tattaattgt ggtcgcacct tcctttctgt
      961 gtactttccg tatagctcac aggattctgg gtacaaaaaa acccgcgcat cggcgggtta
     1021 agcagcgtgg caatgtaacc actcttatca tgatatgcag atttttacga tcgtaaacta
     1081 ttttttcgct gataaaatac agaggttctc cctcccggca attcacgctc aacataccga
     1141 tccatctcaa gcctcactcc cagcatcatc agcatgcctt caacaatccc ctccgctttg
     1201 tgaaggcgtt tacctataca ggtgtcagag cacccatgtt tccgtgccag cgccatgaac
     1261 gtctccccca acacgtaata atcaaccagc aagtcatgca gatcgctgtt gttcctgtta
     1321 aggcgagcca tacacccgca tataatcatc gcgtcatcgt cacaacactg tggacgtgat
     1381 tttacttttt cggggatcag tcccttaaat ccggcagcaa tgggcgacca tgtaacatcc
     1441 tcatggttat ttgccgccca tgccccccag cgctcaagaa cctgccggat atcacgcatc
     1501 agtatcttta ccccatccgc gatgaaccat aaggacgcca ttgacgacgg cgtgcttttt
     1561 cccttcttta tcgccaatgt attttctgac tgtggcacga ttgcagttca gtattcgggc
     1621 tacctcggac tgatttccat atgcctcaag gagcatgtca ggaatggttt ttacggtgaa
     1681 cgtcatgcgg cctcacttct gctgtttcgc aggtctttga gtttctgttg gtaccctgcc
     1741 ttgatcgcct tgcactcttc gatagtccag cgatggcggt tatggtttga ttcgatttcg
     1801 tctactgctt cctgcccgat gcggttaatc agttcgacgc gatacggaac gagatttccg
     1861 cttttatgtt ggttgcacac cacgcattgc ttgtga
//
LOCUS       DAZKHF020000441         1892 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2201S31241
            SAMN43267800-rid25106413.denovo.444, whole genome shotgun sequence.
ACCESSION   DAZKHF020000441 DAZKHF020000000
VERSION     DAZKHF020000441.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267800
            Sequence Read Archive: SRR30307263
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1892)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1892)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 1892)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350581.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350581.2
            Genome Coverage       :: 151x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:21:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,652
            CDSs (total)                      :: 5,557
            Genes (coding)                    :: 5,320
            CDSs (with protein)               :: 5,320
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 237
            CDSs (without protein)            :: 237
            Pseudo Genes (ambiguous residues) :: 0 of 237
            Pseudo Genes (frameshifted)       :: 102 of 237
            Pseudo Genes (incomplete)         :: 130 of 237
            Pseudo Genes (internal stop)      :: 52 of 237
            Pseudo Genes (multiple problems)  :: 41 of 237
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1892
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267800-rid25106413.denovo.444"
                     /serotype="O 26:H11"
                     /isolate="2201S31241"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-01"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            <1..414
                     /locus_tag="ACHOSC_RS24460"
                     /old_locus_tag="pgaptmp_004892"
     CDS             <1..414
                     /locus_tag="ACHOSC_RS24460"
                     /old_locus_tag="pgaptmp_004892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="secretion protein EspJ"
                     /protein_id="HGU4550652.1"
                     /translation="QSNQFTDLKNKTIQGHKDTVAKVIDWYNPQKNAFGIMMGTPRRS
                     ADIAKEESRNALNFMIMEKDTFNEKILNSNANLQKKYGTTENSSWVSASVGSLLDKGA
                     KVYPDISCSLRLGEPFIITLPETVRLDVNIHPLKK"
     gene            complement(427..648)
                     /locus_tag="ACHOSC_RS24465"
                     /old_locus_tag="pgaptmp_004893"
     CDS             complement(427..648)
                     /locus_tag="ACHOSC_RS24465"
                     /old_locus_tag="pgaptmp_004893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001346876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4550653.1"
                     /translation="MDIQCTLANTFPNEHPEAFILVTSAEKFIVDGNCIQLIGIGNEQ
                     GEFDFYRQIYPVTGGEHKVEALMWTHLPA"
     gene            complement(732..1112)
                     /locus_tag="ACHOSC_RS24470"
                     /old_locus_tag="pgaptmp_004894"
     CDS             complement(732..1112)
                     /locus_tag="ACHOSC_RS24470"
                     /old_locus_tag="pgaptmp_004894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001002868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4550654.1"
                     /translation="MNEIRSNPVKFLEEHLILNTQRYAHHDETSLITVNITREDGLLR
                     LKETEYDMAGSDYILFTAMRDTDSPERFDAPAVVSLSNQCISLRRNDISQTIQQSSPL
                     WLTNQQSGCSVLIVRHGLSESGEQ"
     gene            complement(1313..1888)
                     /locus_tag="ACHOSC_RS24475"
                     /old_locus_tag="pgaptmp_004895"
     CDS             complement(1313..1888)
                     /locus_tag="ACHOSC_RS24475"
                     /old_locus_tag="pgaptmp_004895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1076 domain-containing protein"
                     /protein_id="HGU4550655.1"
                     /translation="MPLTSDIGSRSFNLGLEVFRARIAANGRGDITVGGETVSIVYDA
                     TNGRFSSSGGNDGLLSELLILGFNNGPRALSERMLSMLSDSGEAQSQESIQDKISQCK
                     FPVSSGNFQCPPESIQCPITLERPEEGVFVKNSDSSAVCTLFDVDALSRVVNDGSVHP
                     LTRAPITPSMIVKPEECKYDPARGSFIIKDS"
BASE COUNT          607 a          417 c          330 g          538 t
ORIGIN      
        1 caaagtaatc agtttactga tttaaaaaac aagacaattc agggtcataa agacactgtc
       61 gcaaaagtca tagactggta taacccacaa aaaaacgcat ttggtatcat gatgggcaca
      121 ccaagacgtt ctgctgatat agcaaaggaa gaatccagaa acgctctaaa tttcatgata
      181 atggaaaagg atacatttaa tgaaaagata cttaacagca atgctaattt acaaaaaaaa
      241 tacggcacaa cagaaaatag tagttgggta tcggcttctg ttggttctct tctggacaaa
      301 ggagccaaag tatatccaga tatatcatgc tctctgagac tgggagaacc attcattatt
      361 acacttccag aaacagtcag gctggatgtt aatatccacc cactcaaaaa atgatcctga
      421 acgtttttat gcaggcaaat gcgtccacat caaggcttca accttgtgct ctccccctgt
      481 cacaggataa atttgcctgt aaaaatcaaa ctctccctgc tcattaccaa taccaatcag
      541 ttgaatgcag ttgccatcta caataaattt ttccgctgac gtaaccaaaa taaaggcttc
      601 cggatgttca ttaggaaagg tgttagcaag agtacactga atatccatct ccaacaaaat
      661 ctcctgatta ttagcgtaaa ctcatcgacg aaatcgttac tatcccctgg gcacatatgt
      721 accattgaat actactgttc tccagattct gataatccat ggcgaacaat aagaacacta
      781 cagccgcttt gctgattagt tagccataac ggtgaacttt gctggatagt ttgagaaata
      841 tcatttcgcc ggaggcttat acactgatta ctcaagctta caactgcagg tgcatcaaag
      901 cgctccggac tatcagtatc acgcatagct gtaaacagaa tataatcact accagccata
      961 tcatattccg tttcttttag tcttaacaag ccatcctctc ttgtaatatt aactgtaatt
     1021 aaagatgtct catcatgatg ggcatagcgc tgtgtattaa gaattaaatg ctcttccaga
     1081 aattttacag ggttactacg tatttcattc attatacacc gaagctctga acgatgcggc
     1141 accagaaagt gcccggtatt ttgtgaaata atactactta ccttgattgt tttctctcca
     1201 gctcatgaaa aataactcgc gtattatatt gttttaccaa ccacaggccc cgaatgcctg
     1261 tggtgttttt atataaatta tcggaagtta atattgtttc atttgaaaca tcttaactat
     1321 cttttataat aaagcttccc cttgcagggt catatttaca ctcttctggt ttaacaatca
     1381 ttgatggggt tattggagct cgtgtcagag ggtgaactga accgtcatta accacgcgag
     1441 aaagtgcatc aacatcaaat aaagtgcata ctgctgaact atctgaattt ttgacaaaca
     1501 ctccttcttc gggtctctct agtgtaattg gacactgaat agactctggc gggcactgga
     1561 aatttcctga actaacagga aacttacatt gagatatttt gtcctgaata ctctcttgcg
     1621 attgtgcttc acctgagtcc gaaagcatac ttagcattct ctcactaagg gctcgaggac
     1681 cattattaaa tcccaaaatc aataactcag aaagcaatcc gtcattaccg ccactggatg
     1741 aaaagcgccc attagtagca tcatacacaa tactgacagt ttcaccacct actgtaatat
     1801 ctccgcgccc attggctgca attcgggcac gaaaaacctc cagcccaaga ttaaatgaac
     1861 gtgatccaat atctgaggtt aatggcataa ca
//
LOCUS       DAZKHF020000442         1883 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2201S31241
            SAMN43267800-rid25106413.denovo.445, whole genome shotgun sequence.
ACCESSION   DAZKHF020000442 DAZKHF020000000
VERSION     DAZKHF020000442.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267800
            Sequence Read Archive: SRR30307263
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1883)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1883)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 1883)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350581.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350581.2
            Genome Coverage       :: 151x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:21:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,652
            CDSs (total)                      :: 5,557
            Genes (coding)                    :: 5,320
            CDSs (with protein)               :: 5,320
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 237
            CDSs (without protein)            :: 237
            Pseudo Genes (ambiguous residues) :: 0 of 237
            Pseudo Genes (frameshifted)       :: 102 of 237
            Pseudo Genes (incomplete)         :: 130 of 237
            Pseudo Genes (internal stop)      :: 52 of 237
            Pseudo Genes (multiple problems)  :: 41 of 237
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1883
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267800-rid25106413.denovo.445"
                     /serotype="O 26:H11"
                     /isolate="2201S31241"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-01"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            <1..>1883
                     /locus_tag="ACHOSC_RS24480"
                     /old_locus_tag="pgaptmp_004896"
     CDS             <1..>1883
                     /locus_tag="ACHOSC_RS24480"
                     /old_locus_tag="pgaptmp_004896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0019062 - virion attachment to host cell
                     [Evidence IEA];
                     GO_process: GO:0046718 - symbiont entry into host cell
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tail fiber protein"
                     /protein_id="HGU4550656.1"
                     /translation="FPPSHAGTITVYEDSRPGTLNDFLGAMTEDDARPEALRRFELMV
                     EEVARNASAVAQNTAAAKKSASDASTSAREAATHATDAAGSARAASTSAGQAASSAQS
                     ASSSAGTASTKATEASKSAAAAESSKSAAATSAAAAKTSETNAAASQQSAATSASTAT
                     TKASEAATSARDASASKEAAKSSETNASSSASSAASSATAAGNSAKAAKTSETNAKSS
                     ETAAGQSASAAAGSKTAAASSASAASTSAGQASASATAAGKSAESAASSASTATTKAG
                     EATEQASAAASSASAAKTSETNAKASETSAESSKTAAASSASSAASSASSASASKDEA
                     TRQASAAKSSATTASTKATEAAGSATAAAQSKSTAESAATRAETAAKRAEDIASAVAL
                     EDASTTKKGIVQLSSATNSTSESLAATPKAVKAAYDLANGKYTAQDATTAQKGIVQLS
                     SATNSTSEMLAATPKSVKAAYDLANGKYTAQDATTAQKGIVQLSSATNSASETLAETP
                     KAVKAANDNANGRVPSARKVNGKALSADITLTPKDIGTLNSTTMSFSGGAGWFKLATV
                     TMPQASSVVSITLIGGAGFNVGSPQQAGISELVLRAGNGNPKGITGALWQRTSTGFTN
                     FAWV"
BASE COUNT          502 a          534 c          551 g          296 t
ORIGIN      
        1 ttcccgccgt cacatgccgg gaccatcacc gtgtatgaag attcccgacc cggtacgctg
       61 aatgattttc tcggtgccat gacggaggat gatgcccgtc ctgaggcact gcgccgtttt
      121 gaactgatgg tggaagaggt ggcgcgtaac gcgtccgcgg tggcacagaa cacggcagcc
      181 gcgaagaagt cagccagcga tgccagcaca tcagcccgtg aggcggcaac ccatgcgact
      241 gatgctgcag gctcagcacg cgcagccagc acgtcagccg gacaggccgc gtcgtcggct
      301 cagtcagcgt cttccagcgc aggaacggca tcaacaaagg ccactgaagc atcaaaaagt
      361 gctgccgctg cagagtcctc aaaaagcgcg gcggccacca gtgccgctgc ggcgaaaacg
      421 tcagaaacga atgcggcagc gtcacaacaa tcagcagcca cttctgcatc caccgcgacc
      481 acgaaagcgt cagaagctgc cacctcagcc cgggatgcgt cggcttcaaa agaagcggca
      541 aaatcatcag aaacgaacgc atcatcaagc gccagtagtg ccgcttcctc ggcaacggcg
      601 gcaggaaatt ccgcgaaggc ggcaaaaacg tccgagacga acgctaagtc ttctgagacg
      661 gcagcgggac agagcgcctc agctgcggca ggctcaaaaa cagcggctgc gtcgtctgcc
      721 agtgccgcgt caacaagtgc cgggcaggcc tcagccagtg ccaccgccgc cggaaaatcg
      781 gcagaaagcg ccgcatcatc cgcttcaaca gccacaacga aggctggcga agccactgaa
      841 caggccagcg cagcagcgag ttctgcttcc gcagcgaaga catccgaaac gaacgcgaaa
      901 gcgtcggaaa ccagcgcaga atcctcaaaa acggctgccg catcgtcagc cagttcggcg
      961 gcgtcatcgg catcatcggc gtctgcttca aaagatgagg cgaccagaca ggcgtcagca
     1021 gcgaagagca gcgccacgac ggcatccacg aaggcgacag aggcagctgg cagtgcgacg
     1081 gcggcagctc agagcaaaag tacggcggaa tccgcggcaa cgcgcgccga gacagcagct
     1141 aaacgggcag aggatattgc atccgccgtg gcgcttgagg atgcgagcac gacgaaaaag
     1201 gggatagtac agctcagcag tgcgactaac agcacttccg agtcactggc ggcaacgcca
     1261 aaagccgtta aggccgcgta tgacctggct aacgggaaat acaccgcaca ggatgcaacg
     1321 acagcacaga aagggatcgt tcagctcagc agcgcgacca acagcacatc tgaaatgctg
     1381 gcggcaacgc caaagtcggt aaaggcagcc tatgaccttg ctaacgggaa atataccgct
     1441 caggacgcta cgacagcaca aaaaggaatt gtccagctca gtagtgcaac caacagcgca
     1501 tctgaaacgc ttgccgagac accgaaagca gtgaaagcag ctaatgataa tgcgaatggt
     1561 cgggtacctt ctgcccgtaa ggtgaatggt aaggcgcttt cagcggatat aacactgacg
     1621 ccgaaagata ttggtacgct taactcaaca acaatgtcat tcagcggtgg tgctggttgg
     1681 ttcaaattag caacggtaac catgccacag gcgagttctg ttgtttcaat tacgttgatt
     1741 ggtggcgcgg gatttaacgt ggggtcacct caacaggcag gtatatctga acttgttttg
     1801 cgtgcaggta atggtaatcc gaaggggatt actggtgctt tatggcagcg cacatcgaca
     1861 gggtttacaa attttgcctg ggt
//
LOCUS       DAZKHF020000443         1875 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2201S31241
            SAMN43267800-rid25106413.denovo.446, whole genome shotgun sequence.
ACCESSION   DAZKHF020000443 DAZKHF020000000
VERSION     DAZKHF020000443.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267800
            Sequence Read Archive: SRR30307263
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1875)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1875)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 1875)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350581.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350581.2
            Genome Coverage       :: 151x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:21:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,652
            CDSs (total)                      :: 5,557
            Genes (coding)                    :: 5,320
            CDSs (with protein)               :: 5,320
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 237
            CDSs (without protein)            :: 237
            Pseudo Genes (ambiguous residues) :: 0 of 237
            Pseudo Genes (frameshifted)       :: 102 of 237
            Pseudo Genes (incomplete)         :: 130 of 237
            Pseudo Genes (internal stop)      :: 52 of 237
            Pseudo Genes (multiple problems)  :: 41 of 237
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1875
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267800-rid25106413.denovo.446"
                     /serotype="O 26:H11"
                     /isolate="2201S31241"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-01"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            131..268
                     /locus_tag="ACHOSC_RS24485"
                     /old_locus_tag="pgaptmp_004897"
                     /pseudo
     CDS             131..268
                     /locus_tag="ACHOSC_RS24485"
                     /old_locus_tag="pgaptmp_004897"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312758.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="arylsulfatase"
     gene            complement(376..1611)
                     /gene="aslB"
                     /locus_tag="ACHOSC_RS24490"
                     /old_locus_tag="pgaptmp_004898"
     CDS             complement(376..1611)
                     /gene="aslB"
                     /locus_tag="ACHOSC_RS24490"
                     /old_locus_tag="pgaptmp_004898"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0018209 - peptidyl-serine modification
                     [Evidence IEA];
                     GO_process: GO:0046500 - S-adenosylmethionine metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic sulfatase maturase"
                     /protein_id="HGU4550657.1"
                     /translation="MLQQVPTRAFHVMAKPSGSDCNLNCDYCFYLEKQSLYREKPVTH
                     MDDDTLEAYVRHYIAASEPQNEVAFTWQGGEPTLLGLDFYRRAVKLQAKYGAGRKISN
                     SFQTNGVLLDDKWCAFLAENHFLVGLSLDGPAEIHNQYRVTKGGRPTHKLVMRALTLL
                     QKHHVDYNVLVCVNRTSAQQPLQVYDFLCDAGVEFIQFIPVVERLADETAASDGLKLH
                     APGDIQGELTEWSVRPDEFGEFLVAIFDHWIKRDVGKIFVMNIEWAFANFVGAPGAVC
                     HHQPTCGRSVIVEHNGDVYACDHYVYPQYRLGNMHQQTIAEMIDSPQQQVFGEDKFKQ
                     LPAQCRSCNVLKACWGGCPKHRFMLDASGKPGLNYLCAGYQRYFRHLPPYLKAMADLL
                     AHGRPASDIMHAHLLVVSK"
     gene            complement(1758..1834)
                     /locus_tag="ACHOSC_RS24495"
                     /old_locus_tag="pgaptmp_004899"
     tRNA            complement(1758..1834)
                     /locus_tag="ACHOSC_RS24495"
                     /old_locus_tag="pgaptmp_004899"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1798..1800),aa:Pro,seq:tgg)
BASE COUNT          477 a          504 c          429 g          465 t
ORIGIN      
        1 ttttctggat aaataaataa tttatttttg tcactattat ttatgtaatc atcctgtcag
       61 ggagagggat ctcaattatc aatgcttaat tacgtcatca ttttgatgac atgaaaccgg
      121 agcattttgc atggaatttt cgttttcacc caaacttctt gttgttgctg tcgccgccgc
      181 tcttcctctc atggccagcg cagcagatac cccgtcaact gccaccgcac gcaaaggctt
      241 tgccggatac gatcacccaa accagtatcc acagaacgct gcaatttatt gaatttgcac
      301 gatcatgtag gccggataag gcgtttacgc cgcatccggc aatcaaccgc aggcggccac
      361 aaaatggccg cttttttact tactcaccac cagcaaatgc gcatgcataa tgtcactggc
      421 cgggcgaccg tgcgccagca aatcagccat tgctttaaga tatggcggta gatggcggaa
      481 ataacgctga tacccggcac acaaataatt cagccccggt ttgccgctgg catcgagcat
      541 gaagcggtgt ttcgggcagc ctccccagca cgcttttaac acgttacaac tgcgacactg
      601 cgccggtaat tgcttaaatt tatcttcacc aaacacctgc tgttgcgggg aatcgatcat
      661 ttctgcaatt gtttgctgat gcatattccc cagccgatat tgcggataaa catagtgatc
      721 gcaggcgtaa acgtcgccgt tgtgctcaac aatcaccgag cgcccacagg ttggctgatg
      781 atggcaaacc gcacccggcg caccgacaaa attggcaaac gcccattcga tattcatcac
      841 gaaaatcttg ccgacgtcgc gtttgatcca gtggtcgaat atcgccacca gaaattcacc
      901 aaattcatcg gggcgcaccg accattccgt cagttccccc tgaatatcac caggcgcatg
      961 tagtttcagt ccatcgctgg cagctgtttc atcagccagg cgctcgacca ccggaataaa
     1021 ctggatgaat tcgactcccg catcgcacaa aaaatcataa acctgcaacg gttgctgcgc
     1081 gctggtgcgg ttgacgcaga ccagcacgtt atagtcgaca tgatgttttt gcagcagcgt
     1141 cagggcacgc atcaccagct tatgcgttgg tctgccacct ttggtcacgc gatattgatt
     1201 gtggatctca gccggaccgt ccagcgataa cccaacaaga aaatgatttt ctgccagaaa
     1261 tgcacaccat ttatcatcga gcagcacgcc gttagtctgg aagctgttac ttatcttcct
     1321 gccagcaccg tatttcgcct gtaactttac ggcacggcgg taaaaatcca gcccgagtaa
     1381 cgttggttcg ccgccctgcc aggtaaaagc cacttcgttt tgcggttcgc tggcagcgat
     1441 atagtgacgg acatacgctt ccagcgtgtc atcgtccata tgcgtgactg gcttttcgcg
     1501 gtaaagggat tgtttttcga gataaaaaca gtagtcacag ttcagattac aatcggaacc
     1561 actcggtttc gccatcacat gaaaagcacg cgttggaacc tgttgcagca tggtcactcc
     1621 ttgcctgaag tcatccttca atacgttaag ggcgaaatca taccgtgggg caatgagtta
     1681 ttacgggcgt ttattgcgaa gggagggtga cgaaatgcac agaaaacaaa aaaccccgcc
     1741 gaagcggggt tcaaaattgg tcggcgagag aggattcgaa cctccgaccc actggtccca
     1801 aaccagttgc gctaccaagc tgcgctactc gccgaaatac tactttttga atttttagtt
     1861 caattcttta aagtc
//
LOCUS       DAZKHF020000444         1871 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2201S31241
            SAMN43267800-rid25106413.denovo.447, whole genome shotgun sequence.
ACCESSION   DAZKHF020000444 DAZKHF020000000
VERSION     DAZKHF020000444.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267800
            Sequence Read Archive: SRR30307263
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1871)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1871)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 1871)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350581.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350581.2
            Genome Coverage       :: 151x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:21:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,652
            CDSs (total)                      :: 5,557
            Genes (coding)                    :: 5,320
            CDSs (with protein)               :: 5,320
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 237
            CDSs (without protein)            :: 237
            Pseudo Genes (ambiguous residues) :: 0 of 237
            Pseudo Genes (frameshifted)       :: 102 of 237
            Pseudo Genes (incomplete)         :: 130 of 237
            Pseudo Genes (internal stop)      :: 52 of 237
            Pseudo Genes (multiple problems)  :: 41 of 237
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1871
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267800-rid25106413.denovo.447"
                     /serotype="O 26:H11"
                     /isolate="2201S31241"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-01"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            61..633
                     /locus_tag="ACHOSC_RS24500"
                     /old_locus_tag="pgaptmp_004900"
     CDS             61..633
                     /locus_tag="ACHOSC_RS24500"
                     /old_locus_tag="pgaptmp_004900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309674.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tail assembly protein"
                     /protein_id="HGU4550658.1"
                     /translation="MKTGAEAIRALATQLPAFRQKLSDGWYQVRIAGRDVSASGLTAQ
                     LHETLPDGAVIHIVPRVAGAKSGGVFQIVLGAAAIAGSFFTAGATLAAWGAAIGAGGM
                     TGILFSLGASMVLGGVAQMLAPKARTPRTQTTDNGKQNTYFSSLDNMVAQGNVLPVLY
                     GEMRVGSRVVSQEISTADEGDGGQVVVIGR"
     gene            694..>1871
                     /locus_tag="ACHOSC_RS24505"
                     /old_locus_tag="pgaptmp_004901"
     CDS             694..>1871
                     /locus_tag="ACHOSC_RS24505"
                     /old_locus_tag="pgaptmp_004901"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HGU4550659.1"
                     /translation="MGKGSSKGHTPREAKDNLKSTQLLSVIDAISEGPIEGPVDGLKS
                     VLLNSTPVLDSEGNTNISGVTVVFRAGEQEQTPPEGFESSGSETVLGTEVKYDTPITR
                     TITSANIDRLRFTFGVQALVETTSKGDRNPSEVRLLVQIQRNGGWVTEKDITIKGKTT
                     SQYLASVVVDNLPPRPFNIRMRRMTPDSTTDQLQNKTLWSSYTEIIDVKQCYPNTALV
                     GVQVDSEQFGSQQVSRNYHLRGRILQVPSNYNPQTRQYSGIWDGTLKPAYSNNMAWCL
                     WDMLTHPRYGMGKRLGAADVDKWALYVIGQYCDQSVPDGFGGTELRITCNAYLTTQRK
                     AWDVLSDFCSAMRCMPVWNGQTLTFVQDRPSDKVWTYNRSNVVMPDDGAPFRYSFSAL
                     "
BASE COUNT          408 a          489 c          596 g          378 t
ORIGIN      
        1 atggcacgca tctgccttta cggggatttg caacgatttg gccgccgcat cgaccttcgt
       61 gtgaaaacgg gggctgaagc catccgcgca ctggccacac agctcccggc gtttcgtcag
      121 aaactgagcg acggctggta tcaggtacgg attgccgggc gggacgtcag cgcgtccggg
      181 ttaacggcgc agttacatga gactctgcct gatggcgctg tgattcatat tgttcccaga
      241 gtcgccgggg ccaagtcagg tggcgtattc cagattgtcc tgggggctgc agccattgcc
      301 ggatcattct ttaccgccgg agccaccctt gcagcatggg gggcagccat tggggccggt
      361 ggtatgaccg gcatcctgtt ttctctcggt gccagtatgg tgctcggtgg tgtggcgcag
      421 atgctggcac cgaaagccag aactccccgt acacagacaa cggataacgg taagcagaac
      481 acctatttct cctcactgga taacatggtt gcccagggca atgttctgcc tgttctgtac
      541 ggggaaatgc gcgtggggtc tcgcgtggtt tctcaggaga tcagcacggc agacgaaggg
      601 gacggtggtc aggttgtggt gattggtcgc tgatgcaaaa tgttttatgt gaaaccgcct
      661 gcgggcggtt ttgtcgttta tggagcgtga ggaatgggta aaggcagcag taaggggcat
      721 accccgcgcg aagcgaaaga taacctgaaa tccacgcagc tgctgagtgt gattgatgcc
      781 atcagcgaag ggccgattga aggtccggtg gatggattaa aaagcgtgct gctgaacagt
      841 acgccggtgc tggacagtga ggggaatacc aacatctccg gcgtcacggt ggtgttccgg
      901 gcaggtgagc aggagcagac accgccggaa ggttttgaat cctccggctc cgagacggtg
      961 ctgggtacgg aagtgaaata tgacacgccg atcacccgca ccatcacgtc ggcaaacatc
     1021 gaccgtctgc gctttacctt cggtgtgcag gcactggtgg aaaccacctc aaagggggac
     1081 cggaatccgt cggaagtccg cctgctggtt cagatacagc gtaacggtgg ctgggtgacg
     1141 gaaaaagaca tcaccattaa gggcaaaacc acctcgcagt atctggcctc ggtggtggtg
     1201 gataacctgc ctccgcgccc gtttaatatc cggatgcgca ggatgacgcc ggacagcacc
     1261 acagaccagc tgcagaacaa aacgctctgg tcgtcataca ccgaaatcat cgatgtgaaa
     1321 cagtgctacc cgaacacggc actggtcggc gtacaggtgg actcggagca gttcggcagc
     1381 cagcaggtga gtcgtaatta tcatcttcgc gggcgcattc tgcaggtgcc gtcgaactat
     1441 aacccgcaga cgcgacaata cagcggtatc tgggacggaa cgttaaaacc agcatacagc
     1501 aacaacatgg cctggtgtct gtgggatatg ctgacccacc cgcgctacgg catggggaaa
     1561 cgtcttggtg cggcggatgt ggacaaatgg gcgctgtatg tcatcggcca gtactgcgac
     1621 cagtcagtgc cggacggctt tggcggcacg gagctgcgca tcacctgtaa tgcgtacctg
     1681 accacacagc gcaaggcgtg ggatgtgctc agtgatttct gctcggcgat gcgctgtatg
     1741 ccggtatgga acgggcagac gctgacgttc gtgcaggacc gaccgtcgga taaggtgtgg
     1801 acctataacc gcagtaatgt ggtgatgccg gatgatggcg cgccgttccg ctacagcttc
     1861 agcgccctga a
//
LOCUS       DAZKHF020000445         1870 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2201S31241
            SAMN43267800-rid25106413.denovo.448, whole genome shotgun sequence.
ACCESSION   DAZKHF020000445 DAZKHF020000000
VERSION     DAZKHF020000445.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267800
            Sequence Read Archive: SRR30307263
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1870)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1870)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 1870)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350581.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350581.2
            Genome Coverage       :: 151x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:21:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,652
            CDSs (total)                      :: 5,557
            Genes (coding)                    :: 5,320
            CDSs (with protein)               :: 5,320
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 237
            CDSs (without protein)            :: 237
            Pseudo Genes (ambiguous residues) :: 0 of 237
            Pseudo Genes (frameshifted)       :: 102 of 237
            Pseudo Genes (incomplete)         :: 130 of 237
            Pseudo Genes (internal stop)      :: 52 of 237
            Pseudo Genes (multiple problems)  :: 41 of 237
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1870
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267800-rid25106413.denovo.448"
                     /serotype="O 26:H11"
                     /isolate="2201S31241"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-01"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            121..1638
                     /gene="lysS"
                     /locus_tag="ACHOSC_RS24510"
                     /old_locus_tag="pgaptmp_004902"
     CDS             121..1638
                     /gene="lysS"
                     /locus_tag="ACHOSC_RS24510"
                     /old_locus_tag="pgaptmp_004902"
                     /EC_number="6.1.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001740376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004824 - lysine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006430 - lysyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysine--tRNA ligase"
                     /protein_id="HGU4550660.1"
                     /translation="MSEQETRGANEAIDFNDELRNRREKLAALRQQGVAFPNDFRRDH
                     TSDQLHEEFDAKDNQELESLNIEVSVAGRMMTRRIMGKASFVTLQDVGGRIQLYVARD
                     SLPEGVYNDQFKKWDLGDIIGARGTLFKTQTGELSIHCTELRLLTKALRPLPDKFHGL
                     QDQEVRYRQRYLDLIANDKSRQTFVVRSKILAAIRQFMVARGFMEVETPMMQVIPGGA
                     SARPFITHHNALDLDMYLRIAPELYLKRLVVGGFERVFEINRNFRNEGISVRHNPEFT
                     MMELYMAYADYHDLIELTESLFRTLAQEVLGTTKVTYGEHVFDFGKPFEKLTMREAIK
                     KYRPETDMADLDNFDAAKALAESIGITVEKSWGLGRIVTEIFDEVAEAHLIQPTFITE
                     YPAEVSPLARRNDVNPEITDRFEFFIGGREIGNGFSELNDAEDQAERFQEQVNAKAAG
                     DDEAMFYDEDYVTALEYGLPPTAGLGIGIDRMIMLFTNSHTIRDVILFPAMRPQK"
     gene            complement(1757..>1870)
                     /gene="ghoT"
                     /locus_tag="ACHOSC_RS24515"
                     /old_locus_tag="pgaptmp_004903"
     CDS             complement(1757..>1870)
                     /gene="ghoT"
                     /locus_tag="ACHOSC_RS24515"
                     /old_locus_tag="pgaptmp_004903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type V toxin-antitoxin system toxin GhoT"
                     /protein_id="HGU4550661.1"
                     /translation="IIWFISHEKTHIRLLSAFLVGITWPMSLPVALLFSLF"
BASE COUNT          516 a          414 c          457 g          483 t
ORIGIN      
        1 gaggtaagcg ttagtttcga taagataaac tgagttatta atagtcgagg cagataatac
       61 agtgtaccga tctgtctctt tatctacgct aaattgaaag ctggatttag aggaaccaaa
      121 atgtctgaac aagaaacacg gggagccaat gaggctattg attttaacga tgaactgaga
      181 aatcgccgcg aaaaactggc ggcactacgt cagcaaggtg tggcgtttcc caatgatttt
      241 cgccgcgacc atacctctga ccagctgcac gaagagtttg atgcgaagga taaccaggaa
      301 ctggaatcct taaacattga agtctcggtt gctggccgaa tgatgacccg tcgtatcatg
      361 gggaaagcct cctttgtaac gttgcaggat gtcggtggcc gtattcaact gtacgttgca
      421 agagatagcc tgccagaagg tgtttataac gatcagttta aaaaatggga tctgggtgac
      481 attatcggtg cccgcggtac gctgtttaag acgcaaaccg gtgagctttc cattcactgt
      541 actgagttgc gcctgctgac aaaagcacta cgtcctttac cagataaatt ccatggtctg
      601 caggatcagg aagtccgtta tcgtcaacgt tatctggacc tcatcgctaa cgataaatcc
      661 cgtcaaacgt ttgttgtccg ttcaaaaatt ctggccgcta tccgccagtt catggtcgcg
      721 cgcggcttta tggaagtaga aaccccgatg atgcaggtaa ttccaggtgg ggcatctgct
      781 cgcccgttta ttacccatca taacgctctg gatttagata tgtacctgcg tatcgcgccg
      841 gagctgtatc tgaaacgtct ggttgtaggc ggttttgaac gggtattcga aatcaaccgt
      901 aacttccgta atgaaggtat ttctgttcgc cataatcctg agttcacaat gatggaactc
      961 tacatggcgt atgcggatta ccacgatttg attgaactga cagagtccct gttccgcacc
     1021 ctggcacaag aggttctggg taccactaaa gtcacttatg gcgagcatgt gtttgatttc
     1081 ggcaaaccgt ttgaaaaact caccatgcgc gaagcaatca aaaaatatcg tccagaaacc
     1141 gatatggccg acctggataa ttttgatgct gctaaagcat tagctgaatc tatcggtatt
     1201 acggtagaga aaagctgggg gctgggacgt attgtcacgg agatctttga tgaagtggca
     1261 gaagcacatc tgattcagcc aacctttatt acggaatatc cggcagaagt gtcaccgctg
     1321 gcacgccgta atgatgttaa cccagaaatc accgaccgtt ttgaattctt catcggtggt
     1381 cgtgaaatcg gtaatggttt tagcgaatta aacgacgcag aagatcaggc tgaacgtttc
     1441 caggaacagg ttaacgctaa agctgcaggt gacgacgaag ccatgttcta tgacgaagat
     1501 tacgtgactg cgctggaata tggtctgccg ccaaccgctg gtctgggtat tggtatcgac
     1561 cgaatgatta tgctgtttac aaacagtcat actattcgcg acgttattct cttcccggcg
     1621 atgcgcccac agaaataaat ttcactttaa tgaacgaagc agtcaggcga ctgcttcgtt
     1681 cgtcgttccg caaatccagt tttgaggaga gacgcttcgt ttcacgataa ccagcgggtc
     1741 cactcaacta cagctcctaa aagagagaaa aaagtaatgc cacaggcaga ctcattggcc
     1801 aggttattcc gaccaggaat gcactaagta aacgaatatg tgttttctca tgtgagataa
     1861 accagataat
//
LOCUS       DAZKHF020000446         1862 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2201S31241
            SAMN43267800-rid25106413.denovo.449, whole genome shotgun sequence.
ACCESSION   DAZKHF020000446 DAZKHF020000000
VERSION     DAZKHF020000446.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267800
            Sequence Read Archive: SRR30307263
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1862)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1862)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 1862)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350581.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350581.2
            Genome Coverage       :: 151x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:21:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,652
            CDSs (total)                      :: 5,557
            Genes (coding)                    :: 5,320
            CDSs (with protein)               :: 5,320
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 237
            CDSs (without protein)            :: 237
            Pseudo Genes (ambiguous residues) :: 0 of 237
            Pseudo Genes (frameshifted)       :: 102 of 237
            Pseudo Genes (incomplete)         :: 130 of 237
            Pseudo Genes (internal stop)      :: 52 of 237
            Pseudo Genes (multiple problems)  :: 41 of 237
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1862
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267800-rid25106413.denovo.449"
                     /serotype="O 26:H11"
                     /isolate="2201S31241"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-01"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            16..717
                     /locus_tag="ACHOSC_RS24520"
                     /old_locus_tag="pgaptmp_004904"
     CDS             16..717
                     /locus_tag="ACHOSC_RS24520"
                     /old_locus_tag="pgaptmp_004904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000387836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HGU4550662.1"
                     /translation="MEALQTTPKTCSLNTYNKVLCDDLDLDSFALTIANLLSAVRTFN
                     LLDDTRLKEVGFDVLEFTHEYALAIASTKQQHSIRSGNKIACIRTKREACGLTTTELA
                     RLLDLDEEIILQWESGEFEPTISMLIPLANVLGCDPLSLLSEKNSESAIRVNTTEVHA
                     ESIGARIKSARTKLGLTESDLARMIHTYSDPINDWECGICEVPADHIVPLASALNCDL
                     MWLLTGKSEAKEQQQ"
     gene            714..914
                     /locus_tag="ACHOSC_RS24525"
                     /old_locus_tag="pgaptmp_004905"
     CDS             714..914
                     /locus_tag="ACHOSC_RS24525"
                     /old_locus_tag="pgaptmp_004905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000147365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4550663.1"
                     /translation="MTGNIHDKYEGLCLAPDSFANNIHNLLCAVIVLQMSDNDAIKRT
                     GDEVLEFARCYAEAAAEKELSS"
     gene            1113..1294
                     /locus_tag="ACHOSC_RS24530"
                     /old_locus_tag="pgaptmp_004906"
                     /pseudo
     CDS             1113..1294
                     /locus_tag="ACHOSC_RS24530"
                     /old_locus_tag="pgaptmp_004906"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000553980.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            1300..>1862
                     /locus_tag="ACHOSC_RS24535"
                     /old_locus_tag="pgaptmp_004907"
     CDS             1300..>1862
                     /locus_tag="ACHOSC_RS24535"
                     /old_locus_tag="pgaptmp_004907"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001373392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4550664.1"
                     /translation="MQYAEFQAEATANGIQTGSMTIDYHDAIRRLDAGEFDTPNVRGL
                     RILQCLAQADEAGLLGKLPVEMKVAQWRWLYVTTFINEEEDKNGTIDVPNEHGTTDRA
                     VIYNGKHGVMTIYPGPIRFALQQYIEWNLIQKYGEAEGMGRALFLYQKMLITYPDKGF
                     IVSDMGREGLELLLDEMINDLNTHGMP"
BASE COUNT          597 a          392 c          410 g          463 t
ORIGIN      
        1 ttgcatggtg atgatatgga agccttacaa acaacaccaa aaacatgtag cctcaatact
       61 tacaacaagg ttttatgtga tgatttagat ctcgattctt ttgcattaac catcgcaaac
      121 ctgctcagtg ctgttcgcac cttcaacctc ctagatgata cgcgattaaa agaggttggg
      181 tttgatgtgc tagaatttac tcatgaatat gctttagcga tcgcatcgac aaaacaacaa
      241 cattctatcc gatcaggcaa caagatagcc tgcatacgca ccaaacgtga agcctgcggc
      301 ttgacgacta ccgaactagc cagactactc gatcttgacg aagagattat tctgcaatgg
      361 gagagtggag agttcgagcc aaccatcagc atgcttatcc ccctggcaaa cgtcctggga
      421 tgcgatccgc tttctctact aagtgaaaaa aacagcgagt cagctattcg tgtaaatacg
      481 actgaagtcc atgcggaaag tattggcgca cgcatcaaaa gcgcccgtac aaagctggga
      541 ttaaccgaat ctgatcttgc ccgcatgatt catacctaca gtgaccccat aaacgactgg
      601 gaatgcggca tctgtgaagt tcctgctgat catatagtac cactggccag tgcgcttaat
      661 tgtgacctga tgtggttgtt aacgggaaaa tcagaagcaa aggagcagca acaatgactg
      721 gcaatatcca tgataagtat gaaggcttat gcctggcacc ggattccttt gcaaacaata
      781 tccataattt attatgcgca gttatcgtat tacaaatgtc agacaacgac gcaataaaaa
      841 gaacaggtga tgaagttctt gaatttgcac gttgctatgc tgaagcagca gctgaaaaag
      901 aactatccag ttaaatagaa caagtcatct ccagatacta tattacggct taatcgccgg
      961 ggattatcac acacttaatc cacaggaggt tttattatga cttttataaa acataaggca
     1021 tcacacaaaa cagcctgcct tattgcacaa cacgggaaaa atcacatgca tattgcctgc
     1081 ttgtttctgc gtaaagcata cgggagataa taatgcatca gaaaacagca gaacacgaac
     1141 aaaccagagt attgctgacc atcaaaaacg ggaaagtaat attaattcgt catgttcatg
     1201 acgatgaatt tgtagaaatc tttcaacatt cctgtttatt gcagaaaagg caggatatga
     1261 cgttattgca ccagcagatg aagatgagga ataaatttca tgcaatacgc tgaattccag
     1321 gctgaagcaa cagccaatgg tatacaaaca ggcagtatga ctattgatta tcacgacgcc
     1381 atacgccgtc tggatgctgg agaattcgat actcctaatg tgcgaggttt acgtatcctt
     1441 cagtgtctcg cgcaagctga cgaagcagga ttactgggaa aacttccggt tgagatgaag
     1501 gttgcccagt ggcgatggtt gtatgtgacg acattcatca acgaagaaga agacaagaac
     1561 ggcacaattg atgtcccgaa tgaacacgga acaacagatc gcgccgtaat atataacggg
     1621 aagcatgggg ttatgacgat atatcccggc cccattcggt ttgccttaca gcagtatatt
     1681 gaatggaatt taattcaaaa atacggcgag gcagaaggaa tgggtagagc gctgtttctt
     1741 tatcagaaaa tgctcattac atacccagat aaaggtttca ttgtttcaga catggggcga
     1801 gaagggcttg aactccttct ggatgaaatg attaacgacc tgaatactca tggtatgcca
     1861 ga
//
LOCUS       DAZKHF020000447         1861 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2201S31241
            SAMN43267800-rid25106413.denovo.450, whole genome shotgun sequence.
ACCESSION   DAZKHF020000447 DAZKHF020000000
VERSION     DAZKHF020000447.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267800
            Sequence Read Archive: SRR30307263
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1861)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1861)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 1861)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350581.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350581.2
            Genome Coverage       :: 151x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:21:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,652
            CDSs (total)                      :: 5,557
            Genes (coding)                    :: 5,320
            CDSs (with protein)               :: 5,320
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 237
            CDSs (without protein)            :: 237
            Pseudo Genes (ambiguous residues) :: 0 of 237
            Pseudo Genes (frameshifted)       :: 102 of 237
            Pseudo Genes (incomplete)         :: 130 of 237
            Pseudo Genes (internal stop)      :: 52 of 237
            Pseudo Genes (multiple problems)  :: 41 of 237
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1861
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267800-rid25106413.denovo.450"
                     /serotype="O 26:H11"
                     /isolate="2201S31241"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-01"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            complement(78..314)
                     /gene="ycgZ"
                     /locus_tag="ACHOSC_RS24540"
                     /old_locus_tag="pgaptmp_004908"
     CDS             complement(78..314)
                     /gene="ycgZ"
                     /locus_tag="ACHOSC_RS24540"
                     /old_locus_tag="pgaptmp_004908"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="regulatory protein YcgZ"
                     /protein_id="HGU4550665.1"
                     /translation="MHQNSVTLDSAGAITRYFAKANLPTQQETLGEIVTEILKDGRNL
                     SRKSLCAKLLCRLEHATGEEEQKHYNALIGLLFE"
     gene            628..1839
                     /gene="bluF"
                     /locus_tag="ACHOSC_RS24545"
                     /old_locus_tag="pgaptmp_004909"
     CDS             628..1839
                     /gene="bluF"
                     /locus_tag="ACHOSC_RS24545"
                     /old_locus_tag="pgaptmp_004909"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="blue light-responsive regulator BluF"
                     /protein_id="HGU4550666.1"
                     /translation="MLTTLIYRSHIRDDEPVKKIEEMVSIANRRNMQSDVTGILLFNG
                     SHFFQLLEGPEEQVKMIYRAICQDPRHYNIVELLCDYAPARRFGKAGMELFDLRLHER
                     DDVLQAVFDKGTSKFQLTYDDRALQFFRTFVLATEQSTYFEIPAEDSWLFIADGSDKE
                     LDSCALSPTINDHFAFHPIVDPLSRRIIAFEAIVQKNEDSPSAIAVGQRKDGEIYTAD
                     LKSKALAFTMAHALELGDKMISINLLPMTLVNEPDAVSFLLNEIKANALVPEQIIVEF
                     TESEVISRFDEFAEAIKSLKAAGISVAIDHFGAGFAGLLLLSRFQPDRIKISQELITN
                     VHKSGPRQAIIQAIIKCCTSLEIQVSAMGVATPEEWMWLESAGIEMFQGDLFAKAKLN
                     GIPSIAWPEKK"
BASE COUNT          546 a          385 c          372 g          558 t
ORIGIN      
        1 tcatatttta tacgttcatt atcagatgtc ttcataacga cttcatagct aacctgttaa
       61 ataatcaggc tgttgcatta ttcaaaaagc aacccaatta gtgcattata atgtttctgt
      121 tcctcttccc cagtcgcgtg ttccagtcgg caaagaagtt tggcacaaag cgactttcga
      181 ctcagattac gtccatcttt caaaatctca gtcacaattt ccccgagagt ttcctgctga
      241 gtaggcaagt tagcttttgc aaagtaacga gtgattgctc ccgcagaatc taaagtcact
      301 gaattttgat gcatgctacg cctctgttaa aaatgttaat caatgttaag tgaggtaaca
      361 atagcaaact tgtacgaaat atgtgtacag aatgataaat taattttttg caattagtgc
      421 taatgcatat aaaacaatgt gttatgataa aacatattgc atcatttaac tgacaatcta
      481 tacaagattt ttcgccaact agtgttcaac tttttccatt ttttatcaat gtaaagaaac
      541 ggttatgttt tggtattaaa agaaagacac aagaagatat gtctgttacc gtcttactct
      601 cgcctcaccc attaccctgg attgttaatg cttaccaccc ttatttatcg tagccatata
      661 cgtgacgacg aacctgtcaa aaaaatcgaa gaaatggttt cgatagcaaa tcgcaggaac
      721 atgcagtctg acgtaacagg gatcttactg tttaatggtt ctcatttttt ccagcttctg
      781 gaaggtccgg aagaacaggt taaaatgata tatcgggcta tatgccagga tccacggcac
      841 tataatattg ttgagctgct gtgcgattac gcgcctgctc gccgttttgg caaagcggga
      901 atggaattat ttgatttgcg cctgcacgag cgagatgacg ttttacaggc cgtattcgac
      961 aaaggcacat caaaatttca gctaacttat gatgacagag cgctacaatt ttttcgtact
     1021 tttgtccttg caaccgaaca atcaacctat ttcgagatcc ctgccgaaga ctcctggctt
     1081 tttatcgctg acggatctga taaagaactt gattcctgtg ccctttcacc aactataaac
     1141 gaccactttg cctttcatcc tattgtcgat cccttatcgc ggcggataat cgcttttgaa
     1201 gccattgtgc aaaaaaatga agatagccca tcagccatag cggttgggca gcgtaaagac
     1261 ggggaaatct acacagcgga tctcaaaagt aaggcgcttg cattcacgat ggcacacgca
     1321 cttgagctcg gtgataaaat gatttcaatc aatctattac ctatgaccct ggttaacgaa
     1381 cctgacgcag tctctttttt acttaatgaa ataaaggcca atgctctggt gcctgaacaa
     1441 atcatcgttg aatttactga aagtgaagtc atatctcggt ttgatgagtt tgccgaagcg
     1501 attaaatcgc taaaggctgc tggtatcagt gtagcaattg atcattttgg cgcaggtttt
     1561 gctggtttgt tactcctgtc acgcttccag cctgacagaa ttaaaatcag tcaggaattg
     1621 attaccaatg ttcataaaag tgggccacgg caggcaatta ttcaggcgat cataaaatgc
     1681 tgtacatcac ttgaaattca agtcagtgct atgggcgtgg caacaccaga agagtggatg
     1741 tggcttgaat ccgcaggaat tgagatgttt cagggagatc tgtttgctaa agctaaattg
     1801 aatggtatcc cttccatagc gtggccagag aaaaaataat tttcagcaca ttctttcaca
     1861 t
//
LOCUS       DAZKHF020000448         1840 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2201S31241
            SAMN43267800-rid25106413.denovo.451, whole genome shotgun sequence.
ACCESSION   DAZKHF020000448 DAZKHF020000000
VERSION     DAZKHF020000448.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267800
            Sequence Read Archive: SRR30307263
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1840)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1840)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 1840)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350581.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350581.2
            Genome Coverage       :: 151x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:21:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,652
            CDSs (total)                      :: 5,557
            Genes (coding)                    :: 5,320
            CDSs (with protein)               :: 5,320
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 237
            CDSs (without protein)            :: 237
            Pseudo Genes (ambiguous residues) :: 0 of 237
            Pseudo Genes (frameshifted)       :: 102 of 237
            Pseudo Genes (incomplete)         :: 130 of 237
            Pseudo Genes (internal stop)      :: 52 of 237
            Pseudo Genes (multiple problems)  :: 41 of 237
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1840
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267800-rid25106413.denovo.451"
                     /serotype="O 26:H11"
                     /isolate="2201S31241"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-01"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            <1..1723
                     /locus_tag="ACHOSC_RS24550"
                     /old_locus_tag="pgaptmp_004910"
     CDS             <1..1723
                     /locus_tag="ACHOSC_RS24550"
                     /old_locus_tag="pgaptmp_004910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="von Willebrand factor type A domain-containing
                     protein"
                     /protein_id="HGU4550667.1"
                     /translation="SSLILSGCGPESENKESQQQQPSTPTDQQVLAAQQAAIKEAEQR
                     SVADKATADAKAKALAQQEAQQYSDKQALQGRLQAAPKYQHAAREKAASQIANPGTAR
                     YKQFDDNPVKQVAQNPLATFSLDVDTGSYANVRRFLNHGLLPPPDAVRVEEIVNYFPY
                     DWDIKDKQSIPATKPIPFAMRYELAPAPWNEQLTLLKIDILAKDHKSEELPASNLVFL
                     IDTSGSMISDERLPLIQSSLKLLVKELREQDNIAIVTYAGDSRIALPSISGSHKAEIN
                     AAIDSLDAEGSTNGGAGLELAYQQAAKGFIKGGINRILLATDGDFNVGIDDPKSIESM
                     VKKQRESGVTLSTFGVGNSNYNEAMMVRIADVGNGNYSYIDTLSEAQKVLNSEMRQTL
                     ITVAKDVKAQIEFNPAWVTEYRQIGYEKRQLRAEDFNNDNVDAGDIGAGKHITLLFEL
                     TLNGQKASIDKLRYAPDNKSVKSDKTKELAWLKIRWKYPQGKESQLVEFPLGPTINAP
                     SEDMRFCAAVAAYGQKLRGSEYLNNTSWQQIKQWAQQAKGEDPQGYRAEFIRLIELAD
                     GVTDISQ"
BASE COUNT          534 a          408 c          468 g          430 t
ORIGIN      
        1 gagtagtttg attttgtcag gatgcgggcc ggaatctgag aataaggaaa gtcagcaaca
       61 acagcccagt acccccacag accagcaagt gcttgccgcg caacaagctg caataaaaga
      121 ggccgagcaa agatccgtcg ccgataaagc cactgcagac gcgaaagcaa aagccttagc
      181 ccagcaagaa gcgcaacaat attcagacaa acaggctttg caggggcgct tacaggcggc
      241 tccaaaatat caacatgcag ccagagaaaa agcagcgtcc caaatcgcaa atccaggaac
      301 cgctcgctac aagcagttcg atgataatcc ggttaagcag gtagcgcaaa acccgttggc
      361 gacgtttagc cttgacgtcg acaccggcag ttatgcgaat gtacgccgtt ttctcaatca
      421 tgggctgtta cctccgccag acgctgtgcg ggtggaggag atagtcaatt atttcccgta
      481 tgactgggat atcaaagaca aacaatctat tccggccact aagccaatac ctttcgctat
      541 gcgctacgaa ctggcacctg cgccgtggaa tgaacagcta acattgctga aaattgatat
      601 cctggcgaaa gatcataaaa gtgaagagtt accagcttct aatctggtct ttcttatcga
      661 cacttctggt tcaatgattt ctgatgaacg tttgccactt atccagtctt cgttgaaatt
      721 attggtcaaa gaacttcgtg agcaggataa cattgccatc gtgacctacg ctggcgactc
      781 ccgtattgcg ttgccttcta tctccggtag tcacaaggcg gaaattaatg ccgcaattga
      841 ttcgctggat gccgaaggca gtaccaatgg cggtgccggg ctggaactgg cttatcagca
      901 ggcggcgaag gggtttatta agggcggcat caatcgcatt ttattagcca ctgacggtga
      961 ctttaacgtt ggcattgacg atccaaaatc gattgaatca atggtcaaaa aacagcggga
     1021 gtctggtgtt actctgtcga cgtttggcgt ggggaatagc aattacaacg aggcaatgat
     1081 ggtgcgaatt gccgatgttg gtaacggcaa ctacagctac attgataccc tctctgaagc
     1141 gcagaaagta ttgaatagtg aaatgcggca gacgttgatt accgtagcaa aagatgtcaa
     1201 agcgcaaatt gagtttaacc ccgcgtgggt aacggaatac cgtcagattg gttatgaaaa
     1261 gcgccaactt cgggcggaag attttaataa cgacaacgtt gatgcggggg atattggcgc
     1321 aggcaaacat ataacgttgt tattcgaatt aacgctgaac gggcaaaaag catcaattga
     1381 taagttacgc tatgccccgg ataacaaatc agtgaaatcg gacaaaacga aagaactggc
     1441 ctggttaaag attcgctgga aatacccgca gggaaaagaa agtcagttag ttgaattccc
     1501 gctggggcca acaataaacg ccccctctga agatatgcgt ttttgcgcag cagtagctgc
     1561 atatgggcaa aagttacgcg gttctgaata cctgaacaat acctcctggc agcagatcaa
     1621 acagtgggct cagcaggcaa aaggggaaga tccacagggt tacagggcgg aatttattcg
     1681 cctgattgaa ctggcggatg gtgtgactga catcagtcag tgatgactgt ttagcaaact
     1741 atgttcgacc agtcagcata tttgctgact ggtcgaattg attaacaatg atgttaactc
     1801 actcttttgc cggatgctct atttttgggt tgtgagtgtt
//
LOCUS       DAZKHF020000449         1830 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2201S31241
            SAMN43267800-rid25106413.denovo.452, whole genome shotgun sequence.
ACCESSION   DAZKHF020000449 DAZKHF020000000
VERSION     DAZKHF020000449.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267800
            Sequence Read Archive: SRR30307263
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1830)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1830)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 1830)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350581.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350581.2
            Genome Coverage       :: 151x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:21:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,652
            CDSs (total)                      :: 5,557
            Genes (coding)                    :: 5,320
            CDSs (with protein)               :: 5,320
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 237
            CDSs (without protein)            :: 237
            Pseudo Genes (ambiguous residues) :: 0 of 237
            Pseudo Genes (frameshifted)       :: 102 of 237
            Pseudo Genes (incomplete)         :: 130 of 237
            Pseudo Genes (internal stop)      :: 52 of 237
            Pseudo Genes (multiple problems)  :: 41 of 237
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1830
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267800-rid25106413.denovo.452"
                     /serotype="O 26:H11"
                     /isolate="2201S31241"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-01"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            complement(<1..>1830)
                     /locus_tag="ACHOSC_RS24555"
                     /old_locus_tag="pgaptmp_004911"
     CDS             complement(<1..>1830)
                     /locus_tag="ACHOSC_RS24555"
                     /old_locus_tag="pgaptmp_004911"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="host specificity protein J"
                     /protein_id="HGU4550668.1"
                     /translation="FGSQQVSRNYHLRGRILQVPSNYNPQTRQYSGIWDGTFKPAYSN
                     NMAWCLWDMLTHPRYGMGKRLGAADVDKWALYVIGQYCDQSVPDGFGGTEPRITCNAY
                     LTTQRKAWDVLSDFCSAMRCMPVWNGQTLTFVQDRPSDKVWTYNRSNVVMPDDGAPFR
                     YSFSALKDRHNAVEVNWIDPNNGWETATELVEDTQAIARYGRNVTKMDAFGCTSRGQA
                     HRAGLWLIKTELLETQTVDFSVGAEGLRHVPGDVIEIFDDDYAGISTGGRVLAVNSQT
                     RTLTLDREITLPSSGTTLISLVDGSGNPVSVEVQSVTDGLKVKVNRVPDGVAEYSVWG
                     LKLPTLRQRLFRCVSIRDNDDGTYAITAVQHVPEKEAIVDNGAHFDGDQSGTVNGVTP
                     PAVQHLTAEVTADSGEYQVLARWDTPKVVKGVSFLLRLTVTADDGSERLVSTARTTET
                     TYRFTQLALGNYRLTVRAVNAWGQQGDPASVSFRIAAPAAPSRIELTPGYFQITATPH
                     LAVYDPTVQFEFWFSETRITDIRQVETTARYLGTALYWIAASINIKPGHDYYFYIRSV
                     NTVGKSAFVEGVGRASDDAEGYLDFFKGKITESHLGKELLEKVD"
BASE COUNT          365 a          587 c          477 g          401 t
ORIGIN      
        1 atcaactttt tccagcagct ctttaccgag atgggattcg gttatcttgc ctttgaaaaa
       61 atccaggtaa ccttccgcat catcgctcgc ccgaccgacg ccctccacga atgccgattt
      121 gccaacggtg ttcacactgc ggatataaaa gtaataatca tggcccggtt tgatattgat
      181 actggcggct atccagtaca gcgccgtacc aagatagcgg gctgtggttt caacctgcct
      241 gatatcggta atccgcgttt ccgagaacca gaactcaaac tgtaccgtcg ggtcataaac
      301 ggcaagatgc ggcgtggcgg ttatctgaaa atagcccggc gtcagctcaa tcctcgacgg
      361 tgctgccggt gcggcaatcc ggaacgatac cgacgccgga tcgccctgct gcccccacgc
      421 atttaccgcc cggactgtca gcctgtagtt ccccagcgcc agttgcgtga agcggtatgt
      481 ggtttccgtc gtccgggccg tgctgaccag ccgctcactg ccgtcatccg ctgttacggt
      541 cagacggagc aggaagctca cgcccttcac caccttcggc gtgtcccagc gcgccagcac
      601 ctgatattcc ccgctgtctg cggtgacttc ggcggtcagg tgctgcaccg ctggcggcgt
      661 gacaccattc accgtgccgc tctggtcgcc gtcaaagtgc gccccgttat ccacgatggc
      721 ttctttttcc ggtacatgct gcacggcagt gatggcatac gtgccgtcat cgttatcacg
      781 gatactcaca cagcggaaca ggcgctgacg caacgtcggc agcttcagcc cccacacact
      841 gtattctgca acgccgtcag gaacccggtt cactttcacc ttaagtccgt cggtgacgga
      901 ctgaacctcc acgctgaccg gattaccact tccgtcaacc aggcttatca gcgtggtacc
      961 ggaggatggc agcgtgattt cacggtcgag cgtcagcgtc cgggtctggc tgttcaccgc
     1021 cagcacgcga ccaccggtgc tgataccggc atagtcatca tcgaagattt caataacatc
     1081 gcccggtaca tggcgaagcc cttcagcacc cacgctgaaa tccacggtct gcgtctccag
     1141 cagttccgtt ttaatcagcc acagcccggc gcggtgtgcc tgcccccggc tggtacagcc
     1201 aaaggcatcc atcttcgtga cattacgacc gtaacgggca atggcctgcg tatcttcaac
     1261 aagctctgtc gccgtctccc agccgttgtt cgggtcaatc cagttcacct caacggcatt
     1321 atggcggtcc ttcagggcgc tgaagctgta gcggaacggc gcgccatcat ccggcatcac
     1381 cacattactg cggttatagg tccacacctt atctgatggt cggtcctgca cgaacgtcag
     1441 cgtctgcccg ttccataccg gcatacagcg catcgccgag cagaaatcac tgagcacatc
     1501 ccacgccttg cgctgtgtgg tcaggtatgc attacaggtg atgcgcggct ccgtgccgcc
     1561 aaagccgtcc ggtactgact ggtcgcagta ctggccgatg acatacagcg cccatttgtc
     1621 cacatccgcc gcaccaagac gtttccccat gccgtagcgt ggatgggtca gcatatccca
     1681 cagacaccag gccatgttgt tgctgtatgc cggtttaaac gttccgtccc agataccgct
     1741 gtattgtcgc gtctgcgggt tatagttcga cggcacctgc agaatgcgcc cgcgaagatg
     1801 ataattacga ctcacctgct ggctgccgaa
//
LOCUS       DAZKHF020000450         1830 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2201S31241
            SAMN43267800-rid25106413.denovo.453, whole genome shotgun sequence.
ACCESSION   DAZKHF020000450 DAZKHF020000000
VERSION     DAZKHF020000450.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267800
            Sequence Read Archive: SRR30307263
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1830)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1830)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 1830)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350581.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350581.2
            Genome Coverage       :: 151x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:21:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,652
            CDSs (total)                      :: 5,557
            Genes (coding)                    :: 5,320
            CDSs (with protein)               :: 5,320
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 237
            CDSs (without protein)            :: 237
            Pseudo Genes (ambiguous residues) :: 0 of 237
            Pseudo Genes (frameshifted)       :: 102 of 237
            Pseudo Genes (incomplete)         :: 130 of 237
            Pseudo Genes (internal stop)      :: 52 of 237
            Pseudo Genes (multiple problems)  :: 41 of 237
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1830
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267800-rid25106413.denovo.453"
                     /serotype="O 26:H11"
                     /isolate="2201S31241"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-01"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            216..410
                     /locus_tag="ACHOSC_RS24560"
                     /old_locus_tag="pgaptmp_004912"
     CDS             216..410
                     /locus_tag="ACHOSC_RS24560"
                     /old_locus_tag="pgaptmp_004912"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010283910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4550669.1"
                     /translation="MDCRTQRLKNRHSRWAETQAHIPLHAFSMSPILRARHHHFRNTG
                     FAFRQCAQKASFSHPSQLKD"
     gene            418..1830
                     /gene="pykF"
                     /locus_tag="ACHOSC_RS24565"
                     /old_locus_tag="pgaptmp_004913"
     CDS             418..1830
                     /gene="pykF"
                     /locus_tag="ACHOSC_RS24565"
                     /old_locus_tag="pgaptmp_004913"
                     /EC_number="2.7.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010346010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004743 - pyruvate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate kinase PykF"
                     /protein_id="HGU4550670.1"
                     /translation="MKKTKIVCTIGPKTESEEMLAKMLDAGMNVMRLNFSHGDYAEHG
                     QRIQNLRNVMSKTGKTAAILLDTKGPEIRTMKLEGGNDVSLKAGQTFTFTTDKSVIGN
                     SEMVAVTYEGFTTDLSVGNTVLVDDGLIGMEVTAIEGNKVICKVLNNGDLGENKGVNL
                     PGVSIALPALAEKDKQDLIFGCEQGVDFVAASFIRKRSDVIEIREHLKAHGGENIHII
                     SKIENQEGLNNFDEILEASDGIMVARGDLGVEIPVEEVIFAQKMMIEKCIRARKVVIT
                     ATQMLDSMIKNPRPTRAEAGDVANAILDGTDAVMLSGESAKGKYPLEAVSIMATICER
                     TDRVMNSRLEFNNDNRKLRITEAVCRGAVETAEKLDAPLIVVATQGGKSARAVRKYFP
                     DATILALTTNEKTAHQLVLSKGVVPQLVKEITSTDDFYRLGKELALQSGLAHKGDVVV
                     MVSGALVPSGTTNTASVHVL"
BASE COUNT          494 a          454 c          429 g          453 t
ORIGIN      
        1 tgaactttca cttatcctca cactgacaac tttggcacca gacgttgcgc aaacagcgaa
       61 gtttttgcgt aaccttttcc ctggaacgtt aaatctttga taacaattta ttgtctaaca
      121 agttgtatat tttttgaaac gctgtttttg ttttcctttt ggattaattt cagcgtataa
      181 tgcgcgccaa ttgactcttg aatggtttca gcactttgga ctgtagaact caacgactca
      241 aaaacaggca ctcacgttgg gctgagacac aagcacacat tcctctgcac gctttttcga
      301 tgtcacctat ccttagagcg aggcaccacc actttcgtaa taccggattc gctttccggc
      361 agtgcgccca gaaagcaagt ttctcccatc cttctcaact taaagactaa gactgtcatg
      421 aaaaagacca aaattgtttg caccatcgga ccgaaaaccg aatctgaaga gatgttagct
      481 aaaatgctgg acgctggcat gaacgttatg cgtctgaact tctctcatgg tgactatgca
      541 gaacacggtc agcgcattca gaatctgcgc aacgtgatga gcaaaactgg taaaaccgcc
      601 gctatcctgc ttgataccaa aggtccggaa atccgcacca tgaaactgga aggcggtaac
      661 gacgtttctc tgaaagctgg tcagaccttt actttcacca ctgataaatc tgttatcggc
      721 aacagcgaaa tggttgcggt aacgtatgaa ggtttcacta ctgacctgtc agttggcaac
      781 accgtactgg ttgacgatgg tctgatcggt atggaagtta ccgccattga aggtaacaaa
      841 gttatctgta aagtgctgaa caacggtgac ctgggcgaaa acaaaggtgt gaacctgcct
      901 ggcgtttcca ttgctctgcc agcactggct gaaaaagaca aacaggacct gatctttggt
      961 tgcgaacaag gcgtagactt tgttgctgct tcctttattc gtaagcgttc tgacgttatc
     1021 gaaatccgtg agcacctgaa agcgcacggc ggcgaaaaca tccacatcat ctccaaaatc
     1081 gaaaaccagg aaggcctcaa caacttcgac gaaatcctcg aagcctctga cggcatcatg
     1141 gttgcgcgtg gcgacctggg tgtagaaatc ccggtagaag aagttatctt cgcccagaag
     1201 atgatgatcg aaaaatgtat ccgtgcacgt aaagtcgtta tcactgcgac ccagatgctg
     1261 gattccatga tcaaaaaccc acgcccgact cgcgcagaag ccggtgacgt tgcaaacgcc
     1321 atcctcgacg gtactgatgc agtgatgctg tctggtgaat ccgcaaaagg taaatacccg
     1381 ctggaagcgg tttctatcat ggcgaccatt tgcgaacgta ccgaccgcgt gatgaacagc
     1441 cgtctcgagt tcaacaatga caaccgtaaa ctgcgcatta ccgaagcggt atgccgtggt
     1501 gccgttgaaa ctgctgaaaa actggatgct ccgctgatcg tggttgctac tcagggcggt
     1561 aaatctgctc gcgcagtacg taaatacttc ccggatgcca ccatcctggc actgaccacc
     1621 aacgaaaaaa cggctcatca gttggtactg agcaaaggcg ttgtgccgca gctggttaaa
     1681 gagatcactt ctactgatga tttctaccgt ctgggtaaag aactggctct gcagagcggt
     1741 ctggcacaca aaggtgacgt tgtagttatg gtttctggtg cactggtacc gagcggcact
     1801 actaacaccg catctgttca cgtcctgtaa
//
LOCUS       DAZKHF020000451         1828 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2201S31241
            SAMN43267800-rid25106413.denovo.454, whole genome shotgun sequence.
ACCESSION   DAZKHF020000451 DAZKHF020000000
VERSION     DAZKHF020000451.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267800
            Sequence Read Archive: SRR30307263
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1828)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1828)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 1828)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350581.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350581.2
            Genome Coverage       :: 151x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:21:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,652
            CDSs (total)                      :: 5,557
            Genes (coding)                    :: 5,320
            CDSs (with protein)               :: 5,320
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 237
            CDSs (without protein)            :: 237
            Pseudo Genes (ambiguous residues) :: 0 of 237
            Pseudo Genes (frameshifted)       :: 102 of 237
            Pseudo Genes (incomplete)         :: 130 of 237
            Pseudo Genes (internal stop)      :: 52 of 237
            Pseudo Genes (multiple problems)  :: 41 of 237
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1828
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267800-rid25106413.denovo.454"
                     /serotype="O 26:H11"
                     /isolate="2201S31241"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-01"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            139..642
                     /locus_tag="ACHOSC_RS24570"
                     /old_locus_tag="pgaptmp_004914"
     CDS             139..642
                     /locus_tag="ACHOSC_RS24570"
                     /old_locus_tag="pgaptmp_004914"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="non-heme ferritin-like protein"
                     /protein_id="HGU4550671.1"
                     /translation="MATAGMLLKLNSQMNREFYASNLYLHLSNWCSEQSLNGTATFLR
                     AQAQSNVTQMMRMFNFMKSVGATPIVKAIDVPGEKLNSLEELFQKTMEEYEQRSSTLA
                     QLADEAKELNDDSTVNFLRDLEKEQQHDGLLLQTILDEVRSAKLAGMCPVQTDQHVLN
                     VVSHQLH"
     gene            complement(721..972)
                     /gene="yecJ"
                     /locus_tag="ACHOSC_RS24575"
                     /old_locus_tag="pgaptmp_004915"
     CDS             complement(721..972)
                     /gene="yecJ"
                     /locus_tag="ACHOSC_RS24575"
                     /old_locus_tag="pgaptmp_004915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416416.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2766 family protein YecJ"
                     /protein_id="HGU4550672.1"
                     /translation="MSQPLNADQELVSDVVACQLVIKQILDVLDVIAPVEVREKMSSQ
                     LKNIDFTNHPAAADPVTMQAIQKAIALIELKFTPQGESH"
     gene            complement(1087..1173)
                     /gene="azuC"
                     /locus_tag="ACHOSC_RS24580"
                     /old_locus_tag="pgaptmp_004916"
     CDS             complement(1087..1173)
                     /gene="azuC"
                     /locus_tag="ACHOSC_RS24580"
                     /old_locus_tag="pgaptmp_004916"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stress response protein AzuC"
                     /protein_id="HGU4550673.1"
                     /translation="MKLRKILKSMFNNYCKTFKDVPPGNMFR"
     gene            1436..1759
                     /gene="yecR"
                     /locus_tag="ACHOSC_RS24585"
                     /old_locus_tag="pgaptmp_004917"
     CDS             1436..1759
                     /gene="yecR"
                     /locus_tag="ACHOSC_RS24585"
                     /old_locus_tag="pgaptmp_004917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YecR-like lipofamily protein"
                     /protein_id="HGU4550674.1"
                     /translation="MRLLILTLSLITLAGCTVTRQAHVSEVDAATGIVRLVYDQAFLQ
                     HAHTDRYVSRGIADRACQQEGYTHAVPFGQPVGNCSLFAGSLCLNTEFTLSYQCHHSA
                     FPVFL"
BASE COUNT          473 a          432 c          406 g          517 t
ORIGIN      
        1 ttcttatttc agatcatcgt cagaattgac tccacgatca catttcggac cggcagaaag
       61 gaattattct gcaaacagta attatggtgt tttgatttat cttgcacctc tccacttctg
      121 gatataagga tattaggtat ggcaaccgct ggaatgcttc tcaaactcaa ctctcaaatg
      181 aaccgcgagt tttacgcatc caatctctac cttcacctga gtaactggtg ttctgaacag
      241 agtctgaacg gcaccgccac tttccttcgc gcccaggcac agagtaatgt gacccaaatg
      301 atgcgcatgt ttaactttat gaagagtgtc ggcgctaccc ccatcgttaa agccattgat
      361 gttcccggtg aaaaactgaa ctctctggaa gaactgttcc aaaaaacgat ggaagaatac
      421 gagcaacgtt ctagtacgtt ggcacagtta gccgatgaag cgaaagaact gaatgatgat
      481 tcaaccgtca atttcctgcg cgatctggaa aaagaacagc agcatgatgg tctgttgctg
      541 caaaccattc ttgatgaagt gcgcagtgcg aaacttgcgg gtatgtgccc tgtgcagacc
      601 gaccaacatg ttctgaatgt cgtgtcacac cagctgcatt gatcatcatc ggcgctaatg
      661 cattgcgccg atgaaggttt tgagaaaccg cagcctcatc tgtttgaagc agcggttttt
      721 ttaatgggat tcaccctgtg gggtaaactt gagttcaata agcgcgatgg ctttttggat
      781 tgcctgcatg gtgaccgggt ctgcggcggc gggatggtta gtaaagtcga tattcttcag
      841 ctgactggac attttttcac gaacttcaac gggcgcgatt acatcgagaa catccagaat
      901 ttgtttgata accaactggc aagcaaccac atcagaaacc agttcctgat cggcattcag
      961 cggctgggac atcgtaaact cctgatagca ttttgaaagc cgttatagta gcggcttcac
     1021 atcttcagcg atagtcacat ccaccgtcat caggacacaa aaaaacctgc cggagcaggt
     1081 tttttattat cggaacatat tgcctggcgg tacgtctttg aacgtcttgc aatagttatt
     1141 gaacatactt ttcaggattt tgcgcagttt catcgcggca ctccgaccat ttgttataca
     1201 ggtgttattc tctttgcgct tataatatga caaccatcac aaaaatcaat ctttatgtga
     1261 tacaaatcac ataaataccc ctttaatgtt ataaaaatga taatcaaaaa acagcccccc
     1321 tatttctgac acctacagat ggcaagaaat agcgcctgcc aggcgtcttt tccggccatt
     1381 gtcgcagcgc tgtaacgcgt aaaatagtgc tttctcttac tcttctggct ggaccatgag
     1441 acttctgatt ctgactcttt cattaataac gctggcgggg tgtacggtta ctcgccaggc
     1501 ccatgtgagc gaagttgacg cggcaacagg tattgtacgg ttggtttatg accaggcttt
     1561 tttgcagcat gcccataccg atcgttatgt cagtcgcggg atcgccgatc gcgcatgcca
     1621 gcaggaaggc tatacccacg cggtcccctt tggtcagcca gtaggcaact gtagcctttt
     1681 tgcgggttcc ttatgtttga atacggaatt cactttgtca tatcagtgcc atcattccgc
     1741 cttccctgtc tttctctaaa tattttaatc agcgagggga tcttcgctga ttaaagaaat
     1801 agcggtaatg cgttttattc ccattcgt
//
LOCUS       DAZKHF020000452         1813 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2201S31241
            SAMN43267800-rid25106413.denovo.455, whole genome shotgun sequence.
ACCESSION   DAZKHF020000452 DAZKHF020000000
VERSION     DAZKHF020000452.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267800
            Sequence Read Archive: SRR30307263
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1813)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1813)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 1813)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350581.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350581.2
            Genome Coverage       :: 151x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:21:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,652
            CDSs (total)                      :: 5,557
            Genes (coding)                    :: 5,320
            CDSs (with protein)               :: 5,320
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 237
            CDSs (without protein)            :: 237
            Pseudo Genes (ambiguous residues) :: 0 of 237
            Pseudo Genes (frameshifted)       :: 102 of 237
            Pseudo Genes (incomplete)         :: 130 of 237
            Pseudo Genes (internal stop)      :: 52 of 237
            Pseudo Genes (multiple problems)  :: 41 of 237
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1813
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267800-rid25106413.denovo.455"
                     /serotype="O 26:H11"
                     /isolate="2201S31241"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-01"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            71..325
                     /gene="bssS"
                     /locus_tag="ACHOSC_RS24590"
                     /old_locus_tag="pgaptmp_004918"
     CDS             71..325
                     /gene="bssS"
                     /locus_tag="ACHOSC_RS24590"
                     /old_locus_tag="pgaptmp_004918"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309465.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biofilm formation regulator BssS"
                     /protein_id="HGU4550675.1"
                     /translation="MEKNNEVIQTHPLVGWDISTVDSYDALMLRLHYQTPNKSELEGT
                     EVGQTLWLTTDVARQFISILEAGIAKIESGDFPVNEYRRH"
     gene            440..1558
                     /gene="solA"
                     /locus_tag="ACHOSC_RS24595"
                     /old_locus_tag="pgaptmp_004919"
     CDS             440..1558
                     /gene="solA"
                     /locus_tag="ACHOSC_RS24595"
                     /old_locus_tag="pgaptmp_004919"
                     /EC_number="1.5.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050131 - N-methyl-L-amino-acid oxidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-methyl-L-tryptophan oxidase"
                     /protein_id="HGU4550676.1"
                     /translation="MKYDLIIIGSGSVGAAAGYYATRAGLNVLMTDAHMPPHQHGSHH
                     GDTRLIRHAYGEGEKYVPLVLRAQTLWDELSRHNEDDPIFVRSGVINLGPADSAFLAN
                     VAHSAEQWQLNVEKLDAQGIMARWPEIRVPDNYIGLFETDSGFLRSELAIKTWIQLAK
                     EAGCAQLFNCPVTVIRHDDDGVTIETVDGEYQAKKAIVCAGTWVKDLLPELPVQPVRK
                     VFAWYQADGRYSVKNKFPAFTGELPNGDQYYGFPAENDALKIGKHNGGQVIHSADERV
                     PFAEVVSDGSEAFPFLRNVLPGIGCCLYGAACTYDNSPDEDFIIDTLPGHDNTLLITG
                     LSGHGFKFASVLGEIAADFAQDKKSDFDLTPFRLSRFQ"
     gene            1606..1719
                     /locus_tag="ACHOSC_RS24600"
                     /old_locus_tag="pgaptmp_004920"
     CDS             1606..1719
                     /locus_tag="ACHOSC_RS24600"
                     /old_locus_tag="pgaptmp_004920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706980.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YceO family protein"
                     /protein_id="HGU4550677.1"
                     /translation="MRRLLHYLINNIREHLMLYLFLWGLLAIMDLIYVFYF"
BASE COUNT          464 a          438 c          438 g          473 t
ORIGIN      
        1 aagttgcttc agataaaaac gcattgaacc tcgaataacg ttgtctagta acacgaatta
       61 gggggccatg atggaaaaaa ataatgaagt cattcagact catccgctcg tagggtggga
      121 catcagcacc gttgatagct atgatgcgct gatgttgcgt ttgcactacc agaccccaaa
      181 taagtccgag ctggaaggga ctgaagttgg tcagacgctc tggttaacca ctgatgttgc
      241 cagacaattt atttcgatat tagaagcagg aatcgccaaa attgaatccg gtgatttccc
      301 ggtaaacgag tatcggcgtc attaacatga catgaccatc cacccaaaag gcacctcacc
      361 ggtgccttta ccatttctgg gttgtatatc tgcattcagt taattactct gcttaaacca
      421 tgagagcaga gagaaaaaaa tgaaatacga tctcatcatt attggcagcg gttccgtagg
      481 cgctgccgcc gggtattatg caacccgcgc cggtttaaac gtgctaatga ccgacgccca
      541 tatgccaccg catcaacacg gcagccacca tggcgatacg cgattaattc gccatgctta
      601 tggtgaaggc gaaaagtatg tcccgctggt cctccgcgcg caaacgctgt gggatgaact
      661 ttcccgccac aacgaagatg atcccatttt tgtacgctct ggtgtcatta acctcggccc
      721 ggctgactcc gcatttctcg ccaacgtcgc ccacagcgcc gaacagtggc aactcaacgt
      781 tgaaaagctc gatgcgcaag ggattatggc ccgctggcca gaaatacgcg tcccggacaa
      841 ctacatcggc ttatttgaga ctgattccgg ttttttgcgc agcgaactgg cgattaaaac
      901 ctggatccaa ctggcgaagg aagcgggctg tgcgcaactg ttcaactgcc cggtcaccgt
      961 aattcgtcat gacgatgatg gcgtaactat tgaaacggtt gacggtgagt atcaggcgaa
     1021 aaaagcgatt gtctgcgcgg gaacatgggt aaaagacctg ctcccggagc tgcctgtcca
     1081 gcccgtacgt aaagtatttg cctggtatca ggccgatggc cgctatagcg tgaagaataa
     1141 attcccggcg tttaccggtg aactgcccaa tggcgatcaa tattatggtt ttccggcaga
     1201 aaacgacgcg ttgaagattg gcaaacataa cggaggccag gttatccatt cagcggatga
     1261 acgtgttccg tttgcggaag tggtcagcga tggttcggaa gccttcccgt tcttgcgcaa
     1321 tgtattgccg ggtatcggtt gctgcctgta cggcgctgcc tgcacctatg ataattcgcc
     1381 tgacgaagat tttattatcg ataccctacc cggccacgat aatacactgc tcattaccgg
     1441 cctgagtggg cacggtttta aatttgcgtc agttttaggg gaaatagctg ccgattttgc
     1501 gcaagacaaa aaaagcgatt ttgatttgac gccattcagg ctttcccgct tccaataatc
     1561 atcattacgg cctccagaat gaggccgttt ttacaggagt atttaatgcg tcggctgttg
     1621 cactatctca tcaataatat tcgcgagcat ctgatgcttt atctttttct ctggggattg
     1681 ttggcaatta tggatctgat ctatgtgttt tatttttagt tttccgcact gacataaact
     1741 gacaaatatt taatctccct ttcatttggc gtgaaaaatt gaatgagatt attcctgctt
     1801 ctacagcact cac
//
LOCUS       DAZKHF020000453         1811 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2201S31241
            SAMN43267800-rid25106413.denovo.456, whole genome shotgun sequence.
ACCESSION   DAZKHF020000453 DAZKHF020000000
VERSION     DAZKHF020000453.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267800
            Sequence Read Archive: SRR30307263
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1811)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1811)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 1811)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350581.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350581.2
            Genome Coverage       :: 151x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:21:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,652
            CDSs (total)                      :: 5,557
            Genes (coding)                    :: 5,320
            CDSs (with protein)               :: 5,320
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 237
            CDSs (without protein)            :: 237
            Pseudo Genes (ambiguous residues) :: 0 of 237
            Pseudo Genes (frameshifted)       :: 102 of 237
            Pseudo Genes (incomplete)         :: 130 of 237
            Pseudo Genes (internal stop)      :: 52 of 237
            Pseudo Genes (multiple problems)  :: 41 of 237
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1811
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267800-rid25106413.denovo.456"
                     /serotype="O 26:H11"
                     /isolate="2201S31241"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-01"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            57..206
                     /locus_tag="ACHOSC_RS24605"
                     /old_locus_tag="pgaptmp_004921"
     CDS             57..206
                     /locus_tag="ACHOSC_RS24605"
                     /old_locus_tag="pgaptmp_004921"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000590537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4550678.1"
                     /translation="MIKIKSILAGLLLSILVPVDAGKYRKGGSVPSQNMVGGIVRRLV
                     IVGMT"
     gene            310..1356
                     /locus_tag="ACHOSC_RS24610"
                     /old_locus_tag="pgaptmp_004922"
     CDS             310..1356
                     /locus_tag="ACHOSC_RS24610"
                     /old_locus_tag="pgaptmp_004922"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309409.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ShlB/FhaC/HecB family hemolysin
                     secretion/activation protein"
                     /protein_id="HGU4550679.1"
                     /translation="MSALLSPLSLQAADVRRSGDEAFIIQQQRQEALEQQLTPSAPDV
                     RLSAPGSFAHKINFPVETPCFQIKQTELKGADALPHWLPLQKIANGAVGHCLGAKGIN
                     LLMSTLQNRLVDHGYVTTRVLAPSQDLKSGILRLVIIPGVVRHVRLTPDSDDYIQLYS
                     SFPAHEGSLLDLRDIEQGLDLGNSRIQGQHTELNATSGNLSTQNAQLSADTLSARTAG
                     QFSSNGGTINADTLQISAQSLSNRKGSLIQTGTGDFSLSLPGSVDNREGLLAANGAVR
                     LDALSLDNRKGKVQAEQSPSLQKSPPTFLKPFVAGVCAALLAVSVAIPGWQFLTQPSP
                     EEQHFTWGNGCKKQ"
     gene            1597..>1811
                     /locus_tag="ACHOSC_RS24615"
                     /old_locus_tag="pgaptmp_004923"
     CDS             1597..>1811
                     /locus_tag="ACHOSC_RS24615"
                     /old_locus_tag="pgaptmp_004923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001536548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4550680.1"
                     /translation="MVTICVDGENKTHKITDWTLWAGNDDREVMLTCHFRSGRKYTRP
                     LSVCQITPTVNLRNVFLERKGNAVTSRA"
BASE COUNT          461 a          389 c          487 g          474 t
ORIGIN      
        1 tgttatatcg atcgataaat ctgtcatgtt gctgtagttc agatcattat tctataatga
       61 tcaaaattaa gtcaattcta gctggtttgt tactcagcat tctggtaccg gttgatgcgg
      121 gtaaataccg gaagggcggt agcgtgccat cgcaaaacat ggtgggaggt atcgtcagga
      181 ggctggtgat tgttggtatg acatgaataa aaggtttttc gtttgactgg aaaattatgt
      241 tgcaggaacg agaggactga ggaacaaagc ttattccggc gctgtccgtc cttgctgtca
      301 ctgacactga tgtctgcttt attatcgcct ttatctcttc aggcagcgga tgtccggcgt
      361 agcggagatg aagcatttat cattcagcag cagcgtcagg aagcccttga gcaacaactg
      421 acgccttcag cccctgatgt tcgcctttct gcacctggct cttttgccca taagattaat
      481 tttcctgttg aaacgccctg ttttcagatt aaacagacgg aactgaaggg ggctgatgcg
      541 ttaccacact ggctgccttt acaaaaaatc gccaacgggg cggtcgggca ttgcctgggg
      601 gcgaaaggaa ttaatctgct gatgagtaca ttgcagaacc gtctggtcga tcatggttat
      661 gtcaccaccc gtgttctggc accttcgcag gatttaaaaa gcggtatcct ccggctggtt
      721 attattcccg gtgttgtgcg acatgtgcgt ctgacaccgg acagtgatga ctatattcag
      781 ttgtattcct cattcccggc acacgaaggt tctctgctgg atttacggga cattgagcag
      841 gggctggatt taggtaacag ccggatacag ggacaacata ctgagctgaa tgcaaccagt
      901 ggaaatctgt ctacacagaa tgcgcaactg agtgccgata cgctttccgc ccggactgcc
      961 gggcagttca gcagtaatgg cggtacgata aatgccgaca cactgcagat atcggcacaa
     1021 agcctgtcaa atcgtaaagg cagtctgatt cagacgggaa caggggattt ttcgctgagt
     1081 ctgccgggaa gcgtggataa ccgggaaggg ctgcttgcgg caaatggcgc ggtgcgtctg
     1141 gatgcactga gccttgataa tcgcaagggg aaagtgcagg cggagcagtc accctccctt
     1201 cagaaatccc cgcccacgtt tctgaaaccg tttgtggctg gtgtctgtgc ggcattgctg
     1261 gcggtcagcg tggctattcc gggatggcag tttctgacac agccatcacc ggaggagcag
     1321 cattttacct gggggaatgg ttgtaaaaag cagtgaaaac agtaaataca gagtatcgtg
     1381 atgaattagt gctcaggggc taccagtatt caataaccag cgtatcggca tcgcattcct
     1441 gtaattctgg tattgactgt tatgactaaa ttcaatgtat atatggcagg cagaaatatg
     1501 ttaacagtcc cggagttgtg gaaaatcctc cggagctaat atcgggagtg ccttaacaat
     1561 tttccgtaag ttaaatgata atttctgagt aagaatatgg tcacgatttg tgttgacggt
     1621 gaaaataaga ctcataaaat taccgactgg acgctgtggg ctggaaatga cgacagagag
     1681 gttatgctga cctgtcattt tcgcagcggg agaaaatata ctcgtccgct ttctgtatgt
     1741 cagataacgc caactgtaaa tttgcgaaat gttttcctcg aaaggaaagg gaacgctgta
     1801 acatcccggg c
//
LOCUS       DAZKHF020000454         1807 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2201S31241
            SAMN43267800-rid25106413.denovo.457, whole genome shotgun sequence.
ACCESSION   DAZKHF020000454 DAZKHF020000000
VERSION     DAZKHF020000454.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267800
            Sequence Read Archive: SRR30307263
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1807)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1807)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 1807)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350581.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350581.2
            Genome Coverage       :: 151x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:21:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,652
            CDSs (total)                      :: 5,557
            Genes (coding)                    :: 5,320
            CDSs (with protein)               :: 5,320
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 237
            CDSs (without protein)            :: 237
            Pseudo Genes (ambiguous residues) :: 0 of 237
            Pseudo Genes (frameshifted)       :: 102 of 237
            Pseudo Genes (incomplete)         :: 130 of 237
            Pseudo Genes (internal stop)      :: 52 of 237
            Pseudo Genes (multiple problems)  :: 41 of 237
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1807
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267800-rid25106413.denovo.457"
                     /serotype="O 26:H11"
                     /isolate="2201S31241"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-01"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            28..1260
                     /locus_tag="ACHOSC_RS24620"
                     /old_locus_tag="pgaptmp_004924"
     CDS             28..1260
                     /locus_tag="ACHOSC_RS24620"
                     /old_locus_tag="pgaptmp_004924"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000777725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maltose ABC transporter substrate-binding
                     protein"
                     /protein_id="HGU4550681.1"
                     /translation="MRLSNIVTVIILAISSTLTPQAMAEKLIPETDAELLIWSDATSA
                     EYMKYAATEFNKDFGYKVKFTFRNIAPMDAASRIMQDGGTTRVADVAEIEHDTLGRLV
                     VAGGVMENMVSAERIKKTFIPGAVSAATYNNISYGFPVSFATLALFYNKDLLNTAPKT
                     FEEINTFSEKFNNSSEHKYALLWDVQNYYVSRMFITLYGANEFGKTGNDPKALGIASS
                     EAKKGLETMKRLKKANPSNPLDMGNPQVLRGLFNEGKVAAVIDGPWSIQGYIDSGINF
                     GVTRIPTLDGHQPRTFSTVRLAVVSSFTEYPHAAELFADYLTTDKMLMKRYEMMNLIP
                     PIDSLMNKISQTGSEAIKAIIAQANYSDAMPSIPEMSYLWSPMTNAILATWVENKTPD
                     EVLNHAQTIIEEQLSLQE"
     gene            1263..>1807
                     /locus_tag="ACHOSC_RS24625"
                     /old_locus_tag="pgaptmp_004925"
     CDS             1263..>1807
                     /locus_tag="ACHOSC_RS24625"
                     /old_locus_tag="pgaptmp_004925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016243614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HGU4550682.1"
                     /translation="MNTQQLKMKSAPVLPISCLIMGGTQLSRHYYVKGGIFFAIQVCF
                     LLYLSDIVHTLIGLFTLGDVAQIRKGLTVIQGDNSIFMLVEGVIATIIVGLFATIYIL
                     NIKDARNSSYCHLTFKQQLYKLYEDKFAFIVLTPAFLASIAFIVLPIVITVLVSFTNY
                     AAPNHIPPKNLVDWVGIKNFI"
BASE COUNT          559 a          341 c          363 g          544 t
ORIGIN      
        1 aaagatatta ttcacgaggt aaatataatg agactttcaa atatcgtcac agttataatt
       61 cttgcaattt ccagtacgct aacaccccag gcgatggcag aaaaattgat tcctgaaact
      121 gatgctgagt tattaatctg gtctgatgcc acaagcgcgg aatatatgaa atatgcagca
      181 acggaattca ataaagattt tggctacaag gtcaagttta catttcgcaa tatagcgcca
      241 atggatgcag catcaagaat tatgcaggat gggggtacga ctcgtgtagc tgatgtagct
      301 gaaattgaac atgataccct ggggcggtta gtcgttgctg gcggggttat ggaaaacatg
      361 gtctcagctg agcggattaa aaaaacattt attccaggcg cagtatcggc agctacatat
      421 aataacatca gctatggttt tcctgtaagt ttcgcaacgc tggcgctttt ctataataag
      481 gatttgttaa acaccgcacc aaaaacattc gaagaaatca atactttcag tgaaaagttt
      541 aataattcat ccgagcataa atatgctctg ctatgggatg tacaaaatta ttatgtttca
      601 cgtatgttta ttaccttgta tggtgccaac gaattcggta aaaccggtaa cgatcctaaa
      661 gctctaggca tcgcttcatc tgaagcgaag aaagggctag agacaatgaa acgcttaaag
      721 aaagcgaatc cttctaatcc tcttgatatg ggtaatccac aagttctaag aggtctgttt
      781 aatgaaggaa aagttgctgc tgtaatcgac ggaccttggt ccatacaagg ttacattgac
      841 agcggaatca attttggcgt gacacgcatc ccaacattag atggtcatca gcctcgcaca
      901 ttttcaacag tacgactggc cgttgtaagc tcatttaccg aatatcctca tgccgctgaa
      961 ttattcgctg actacctgac aacggataag atgttgatga agcgttatga aatgatgaat
     1021 ctcatcccac caatcgattc acttatgaac aaaattagcc agacaggtag tgaagctata
     1081 aaagcaatta ttgctcaagc taattattct gacgcaatgc catcaatacc ggaaatgtct
     1141 tatctatggt ctccaatgac taatgctatt ttggctacct gggttgagaa taaaacaccg
     1201 gatgaagttt taaatcatgc acaaacaatt attgaagaac aactttcgct tcaggagtaa
     1261 ttgtgaatac acaacaactg aaaatgaaga gtgcacctgt gctacctatt tcctgtttga
     1321 ttatgggcgg gactcagtta tcacgtcatt attatgtgaa aggcgggata ttctttgcaa
     1381 tacaagtatg ctttttgctt tatctttccg atattgtgca tacattaata ggtttgttca
     1441 cattaggtga tgtcgcgcaa attcgaaaag ggttgactgt tattcagggt gacaattcta
     1501 tatttatgct tgtagagggc gtgatagcga ccattattgt tggcttattc gccactattt
     1561 acatccttaa tattaaagat gcgcggaact catcttattg ccatctgact tttaaacaac
     1621 agttatataa gctctatgag gataaatttg catttatagt cctaacgccc gcatttttag
     1681 cgagtatagc attcattgtt ttacctattg taattacagt tcttgtatcg tttacaaact
     1741 atgctgcgcc aaatcacatc ccaccaaaga acctggttga ttgggttggg attaaaaatt
     1801 tcattat
//
LOCUS       DAZKHF020000455         1800 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2201S31241
            SAMN43267800-rid25106413.denovo.458, whole genome shotgun sequence.
ACCESSION   DAZKHF020000455 DAZKHF020000000
VERSION     DAZKHF020000455.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267800
            Sequence Read Archive: SRR30307263
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1800)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1800)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 1800)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350581.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350581.2
            Genome Coverage       :: 151x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:21:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,652
            CDSs (total)                      :: 5,557
            Genes (coding)                    :: 5,320
            CDSs (with protein)               :: 5,320
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 237
            CDSs (without protein)            :: 237
            Pseudo Genes (ambiguous residues) :: 0 of 237
            Pseudo Genes (frameshifted)       :: 102 of 237
            Pseudo Genes (incomplete)         :: 130 of 237
            Pseudo Genes (internal stop)      :: 52 of 237
            Pseudo Genes (multiple problems)  :: 41 of 237
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1800
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267800-rid25106413.denovo.458"
                     /serotype="O 26:H11"
                     /isolate="2201S31241"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-01"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            complement(<1..1124)
                     /locus_tag="ACHOSC_RS24630"
                     /old_locus_tag="pgaptmp_004926"
     CDS             complement(<1..1124)
                     /locus_tag="ACHOSC_RS24630"
                     /old_locus_tag="pgaptmp_004926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012907106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="secretion protein EspD"
                     /protein_id="HGU4550683.1"
                     /translation="MLNVNSDIQSMRSGASAATATSGINQPEVTSALDLQLVKSTAPS
                     ASWTESTALPTPPAGHSLVTPSVAEDVLSKLFGGISGEVRSRTEGTEPQRSTQNASSG
                     YPYLSQVNNVDPQAMMMMVTLLSLDASAQKVASMKNSNEIYTDGQNKALDNKTLEFKK
                     QLEEQQKAEEKAQKSKILGQVFGWLGVAVTAIAAIFNPVLWAVVAVSATAMALQTAVD
                     VMGDKAPQALKTAAQVFGGVSIAASILTAGIGGVSSLMSKVGDVANKVGSNIVKTVTK
                     LADVFVENVVSKVAATANGFTTSARSIGTTVLNNDSAQYSILAQLSAYAVQNLTRQSE
                     NLGESAKVELDKAAAELRNQASYLQNASQLISDSARVNSR"
     gene            complement(1137..1715)
                     /gene="espA"
                     /locus_tag="ACHOSC_RS24635"
                     /old_locus_tag="pgaptmp_004927"
     CDS             complement(1137..1715)
                     /gene="espA"
                     /locus_tag="ACHOSC_RS24635"
                     /old_locus_tag="pgaptmp_004927"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system LEE translocon
                     filament protein EspA"
                     /protein_id="HGU4550684.1"
                     /translation="MDTSTATSVASANASTSTSTVYDLGSMSKDEVVQLFNKVGVFQA
                     ALLMFAYMYQAQSDLSIAKFADMNEASKESTTAQKMANLVDAKIADVQSSSDKNKKAK
                     LPQEVIDYINDPRNDITVSGISDLNAELGAGDLQTVKAAISAKSNNLTTVVNNSQLEI
                     QQMSNTLNLLTSARSDIQSLQYRTISAISLGK"
BASE COUNT          475 a          433 c          359 g          533 t
ORIGIN      
        1 atacggctat ttactcgtgc tgagtcggat attaactgag aagcattttg taaatagctt
       61 gcctggtttc gcaactcggc tgcagcttta tccagctcaa ccttcgcact ctcacccagg
      121 ttttcactct gtcgagttaa gttttgtacc gcgtaagcag ataactgagc caatatgctg
      181 tattgcgcag aatcattatt cagcacagtt gtcccaatag aacgtgcaga ggtcgtaaat
      241 ccatttgccg ttgccgcaac ttttgaaaca acgttctcaa caaatacatc ggccagttta
      301 gtaactgttt tcacgatgtt tgaaccaact ttgttagcga catcgccaac tttagacata
      361 agtgaagaca ccccgccaat gccggctgtc agaatacttg cagcaataga tactccgcca
      421 aagacttgcg ctgctgtctt taacgcctgc ggggccttat cgcccattac atcaactgca
      481 gtttgcagtg ccattgctgt tgcgctaacg gcaacaacag cccagagtac cgggttaaaa
      541 atagctgcaa tagcagttac cgcgacgccc aaccaaccaa agacctgacc aagaatttta
      601 cttttttgtg ctttctcttc ggctttctgt tgttcttcga gctgcttttt aaactccagc
      661 gttttattat ccagcgcctt attttgccca tccgtgtaga tctcgttgga gtttttcata
      721 ctcgcaactt tctgcgctga agcatccagg gataacaggg tgaccatcat catcatggcc
      781 tgagggtcaa cgttattcac ctgagaaaga taaggatagc cagagcttgc attctgagtg
      841 ctgcgctgtg gttcggttcc ctctgttcga ctcctaactt caccactaat accaccaaac
      901 agtttactca ggacatcctc agcaacagag ggcgtcacta atgagtggcc tgccggtggc
      961 gttggtaaag cggtactttc tgtccaactt gctgatgggg ccgtggattt aaccagttgt
     1021 aaatccagcg cggaagtcac ttcaggctga ttaatacctg aagtagccgt agcagcgctg
     1081 gccccagacc tcatagactg gatatcgcta tttacattaa gcatagttat ctccggttat
     1141 ttaccaaggg atattgctga aatagttctg tattgcagtg actgaatatc agaacgtgca
     1201 ctcgttaata ggtttaacgt atttgacatt tgctgtattt caagctggct attattcact
     1261 accgtggtca agttattcga tttggccgaa atagcggcct tcaccgtttg caaatcacca
     1321 gcgcctaatt cagcatttag atcgctaata ccacttactg taatgtcatt gcgaggatca
     1381 tttatatagt caatcacttc ttgaggaagt ttggctttct tattcttgtc agaactactc
     1441 tgaacatcag caattttagc atccacaaga ttagccattt tttgggctgt ggttgactcc
     1501 ttagatgcct cattcatatc agcaaacttt gcaatcgaca gatcgctttg tgcctgatac
     1561 atataggcaa acatgagaag cgcagcctga aaaacaccga ctttattaaa tagctgaact
     1621 acttcgtctt tcgacatact gcctaagtca tagactgtcg atgtcgaagt actcgcgttc
     1681 gcactagcaa ctgatgttgc agttgatgta tccatgtata tacctcttaa taatctttct
     1741 tagcaagacc aaaaacgatt aaaaaaccaa tatcaaataa catcctcctt ataatcaatt
//
LOCUS       DAZKHF020000456         1791 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2201S31241
            SAMN43267800-rid25106413.denovo.459, whole genome shotgun sequence.
ACCESSION   DAZKHF020000456 DAZKHF020000000
VERSION     DAZKHF020000456.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267800
            Sequence Read Archive: SRR30307263
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1791)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1791)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 1791)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350581.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350581.2
            Genome Coverage       :: 151x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:21:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,652
            CDSs (total)                      :: 5,557
            Genes (coding)                    :: 5,320
            CDSs (with protein)               :: 5,320
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 237
            CDSs (without protein)            :: 237
            Pseudo Genes (ambiguous residues) :: 0 of 237
            Pseudo Genes (frameshifted)       :: 102 of 237
            Pseudo Genes (incomplete)         :: 130 of 237
            Pseudo Genes (internal stop)      :: 52 of 237
            Pseudo Genes (multiple problems)  :: 41 of 237
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1791
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267800-rid25106413.denovo.459"
                     /serotype="O 26:H11"
                     /isolate="2201S31241"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-01"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            155..1588
                     /locus_tag="ACHOSC_RS24640"
                     /old_locus_tag="pgaptmp_004928"
     CDS             155..1588
                     /locus_tag="ACHOSC_RS24640"
                     /old_locus_tag="pgaptmp_004928"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfjI family protein"
                     /protein_id="HGU4550685.1"
                     /translation="MCLLAPENPYPIYALPPLVRNAIIETQKNTQAPLAMVATSALTA
                     ISIACQNQIDVCRPGNLHGPVNLYSLILADSGERKTTVDKVFMKAFYLRDEALADEYA
                     KLVENYSTEKEIWEQKQKALESKFHKEIRAGKDYKATESELETHLNKPPVPPQIRRTI
                     FNETTIEGMLKYYSDSNRSFALVSSEGGVIFDSRAMSKLGIINTLWDGGSLFIDRKSS
                     PGINLKEPRLTISVMIQPDVYHKGFCTRKKEIVKTSGHHARFLMCQPTSTQGTRIITG
                     DNYSSQYQDLFEERINELIDESLAMSGERRCLHFSPQAARIWTDYYNDVESKLGGLGP
                     LRHCREYAAKNAEYMARLAGLIYHSSGEEGEISPYTAEMARELAIWYGNEYVRLSNPL
                     TFDNSALTVPVRLIPEELELFNWIKSYCIEKGILCMKKNDILQRGPNRFRKKDKINWL
                     LDLLYEQNRVVPVIEGKTLCVAPNFDL"
BASE COUNT          543 a          326 c          422 g          500 t
ORIGIN      
        1 gtcttcactg aattaacctt atcggtttat taaacatcag atctacaaat gctctgtatt
       61 tctcagagca tcaggtctga atagcagcta ttttcgctat tgtagcggga tttgctgcta
      121 ttttactaac ccattaatga aaagagaggc ttttatgtgc ttacttgcac cagaaaatcc
      181 ttatccgata tatgcactac caccgctggt gagaaatgca ataattgaaa ctcaaaaaaa
      241 tacacaggca cctttggcta tggtggcaac atccgcatta acagcgatct caattgcctg
      301 tcagaatcag attgacgtgt gcagacctgg aaatttacat gggcctgtta atctttactc
      361 tctgattctg gctgattccg gtgaaaggaa aacgaccgtg gataaagtgt ttatgaaagc
      421 attttatctc agggatgaag ccctggcgga tgaatacgcg aaactggttg agaattacag
      481 tacagaaaag gaaatatggg agcaaaaaca aaaagcgctg gaatcaaaat ttcataaaga
      541 gattcgtgcc ggtaaagatt acaaggcaac agaatcagag cttgaaacgc atctgaataa
      601 acctcctgtt ccgccgcaga tacgtcgaac aatttttaat gagacaacga tagagggaat
      661 gttaaaatat tactccgata gcaatcgctc ttttgctctt gtatccagtg aagggggggt
      721 aatttttgac agccgggcca tgagtaaact gggaattatt aacactttgt gggatggagg
      781 ttctcttttc atcgacagga aatcatctcc cggaattaat ttgaaggagc caagactgac
      841 gatatcggtg atgattcagc ctgatgttta ccacaaaggt ttttgtacgc gaaaaaaaga
      901 aattgtgaaa acgtcaggac atcatgcaag gtttttgatg tgtcaaccaa catcaacgca
      961 ggggacaagg ataataaccg gagataatta ttcatcacag tatcaggatt tgtttgaaga
     1021 gaggatcaat gagcttattg atgaaagtct ggcgatgagt ggtgaacgac gttgccttca
     1081 cttttctcct caggctgcca gaatctggac ggattattac aatgatgtgg aatcgaagct
     1141 ggggggattg gggcctttaa gacattgccg ggaatatgct gctaaaaatg cagagtatat
     1201 ggcaagactg gcaggactta tttaccattc gagcggtgaa gagggggaga tttcccctta
     1261 cactgcagaa atggcgagag aattagcaat atggtacggt aatgagtatg tgcggttgtc
     1321 taatccgtta acttttgaca actctgccct gaccgtacct gtgcgactca ttccagagga
     1381 gcttgaactt ttcaactgga taaaaagcta ttgtattgag aaggggatcc tctgtatgaa
     1441 aaaaaatgac attttacagc gtggtccgaa ccgtttccgg aaaaaggata aaatcaactg
     1501 gttactggat ttactgtatg aacaaaacag agttgtaccg gttattgagg gaaaaacgtt
     1561 gtgtgtcgca cctaactttg acctctgaac gacttaatcg ttgagtacat atagtctcct
     1621 ctgctacttg cggaggagac taatgtcttt accatgtcag cgttgtatta gaaaactctg
     1681 gtacaggtca gtcagtaaat ataactggtg taaccactat ttactattgg atagagtgtt
     1741 actcaggata ttgtcggctt tagtctgagg tgaggtgggg ccttcattgt t
//
LOCUS       DAZKHF020000457         1756 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2201S31241
            SAMN43267800-rid25106413.denovo.460, whole genome shotgun sequence.
ACCESSION   DAZKHF020000457 DAZKHF020000000
VERSION     DAZKHF020000457.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267800
            Sequence Read Archive: SRR30307263
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1756)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1756)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 1756)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350581.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350581.2
            Genome Coverage       :: 151x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:21:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,652
            CDSs (total)                      :: 5,557
            Genes (coding)                    :: 5,320
            CDSs (with protein)               :: 5,320
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 237
            CDSs (without protein)            :: 237
            Pseudo Genes (ambiguous residues) :: 0 of 237
            Pseudo Genes (frameshifted)       :: 102 of 237
            Pseudo Genes (incomplete)         :: 130 of 237
            Pseudo Genes (internal stop)      :: 52 of 237
            Pseudo Genes (multiple problems)  :: 41 of 237
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1756
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267800-rid25106413.denovo.460"
                     /serotype="O 26:H11"
                     /isolate="2201S31241"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-01"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            <1..375
                     /locus_tag="ACHOSC_RS24645"
                     /old_locus_tag="pgaptmp_004929"
     CDS             <1..375
                     /locus_tag="ACHOSC_RS24645"
                     /old_locus_tag="pgaptmp_004929"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysozyme"
                     /protein_id="HGU4550686.1"
                     /translation="GAIMVDGKPVIPGMKLSKEKCDRVNAIERDKALAWVERNIKVPL
                     TEPQKAGIASFCPYNIGPGKCFPSTFYKRLNAGDRRGACEAIRWWIKDGGRDCRIRSN
                     NCYGQVSRRDQESALACWGIDR"
     gene            650..1219
                     /locus_tag="ACHOSC_RS24650"
                     /old_locus_tag="pgaptmp_004930"
     CDS             650..1219
                     /locus_tag="ACHOSC_RS24650"
                     /old_locus_tag="pgaptmp_004930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage antirepressor N-terminal domain-containing
                     protein"
                     /protein_id="HGU4550687.1"
                     /translation="MNMMTVPFHGDSLYVVNHNGEPYVPMKPVVAGMGLAWQSQLAKL
                     RQRFASTITEIVMVAEDGKRRNMVSLPLRKLAGWLQTINPNKVKPEIRGKVIQYQEEC
                     DDVLYEYWTKGFVVNPRRMSVMEELNQACADMKRDKNIASVFATGLNEWKQVKSAHVS
                     KIRTLINEANLLIDFVLADTDKGKITKAD"
     gene            1219..1368
                     /locus_tag="ACHOSC_RS24655"
                     /old_locus_tag="pgaptmp_004931"
     CDS             1219..1368
                     /locus_tag="ACHOSC_RS24655"
                     /old_locus_tag="pgaptmp_004931"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000455400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4550688.1"
                     /translation="MEWWLMISDKLITLAKILCVIVGISFLVILVAIFFSTAWRVLTL
                     SGLVG"
     gene            1376..1696
                     /locus_tag="ACHOSC_RS24660"
                     /old_locus_tag="pgaptmp_004932"
                     /pseudo
     CDS             1376..1696
                     /locus_tag="ACHOSC_RS24660"
                     /old_locus_tag="pgaptmp_004932"
                     /inference="COORDINATES: protein motif:HMM:NF015218.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="lysis protein"
     gene            1698..1756
                     /locus_tag="ACHOSC_RS24665"
                     /old_locus_tag="pgaptmp_004933"
                     /pseudo
     CDS             1698..1756
                     /locus_tag="ACHOSC_RS24665"
                     /old_locus_tag="pgaptmp_004933"
                     /inference="COORDINATES: protein motif:HMM:NF017860.4"
                     /note="In lambda-like phage, genes encoding the i-spanin
                     subunit Rz, and the o-spanin subunit Rz1 (this protein)
                     overlap.; incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_process: GO:0019835 - cytolysis [Evidence IEA];
                     GO_process: GO:0044659 - viral release from host cell by
                     cytolysis [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="lysis system o-spanin lipoprotein Rz1"
BASE COUNT          467 a          381 c          487 g          421 t
ORIGIN      
        1 ggcgccatca tggtggatgg taagcctgtg attcctggca tgaagctgtc gaaggaaaaa
       61 tgcgaccggg ttaacgctat cgaacgggat aaggcgctgg catgggtgga gcgtaatata
      121 aaagttccac tgaccgagcc acaaaaagca ggtatcgcgt cattttgccc ctataacatt
      181 ggccccggta agtgtttccc gtcgacgttt tataagcggc ttaatgcagg cgatcgcagg
      241 ggagcgtgtg aggcgattcg ctggtggatt aaggacggtg gcagagactg ccgtatccgt
      301 tcaaacaact gttacggtca ggtatcaaga cgtgaccagg agagtgcgct ggcatgctgg
      361 ggtatcgaca ggtaagcaga atattttgct gaaaaatgac gttggccaac gcgggtagac
      421 aacacgaaat cctgcgaact ggcaaaatgt aagtgaataa agtcaacaag attgtttcat
      481 gaagaggcac cgtaatggtg cctttgtcat ttctgcgctt cgcacaagcg taaataaacc
      541 aaagaacctt tcaggatgag ccctggtgga taaccggcag tggtctggtt aaccctcttt
      601 gggctggtta ttcctgtgcg cagggttcat cactaaaagg aaataaccga tgaatatgat
      661 gaccgtgccg tttcacggcg attctcttta tgtggttaac cataacggcg aaccatacgt
      721 tcccatgaaa cctgtcgttg cggggatggg gctggcctgg caatcacagt tggctaagtt
      781 aagacagcgt tttgcgtcaa ctataacgga aatcgttatg gttgctgagg atgggaaacg
      841 acgcaatatg gtgtccctgc cgcttcgaaa acttgcaggc tggttacaaa ccatcaatcc
      901 caacaaagta aaacccgaaa tccgcggcaa ggtaatccag tatcaggaag agtgtgacga
      961 tgttctctat gaatactgga cgaagggttt tgtcgttaat ccccgtcgaa tgagtgtgat
     1021 ggaagaactc aatcaggctt gcgctgacat gaaacgggat aaaaacattg ccagtgtgtt
     1081 tgctaccggg ctgaatgagt ggaaacaggt taaatccgcg catgtatcaa aaatccgcac
     1141 attgataaac gaagcgaatc tgctgattga ttttgtcctg gctgatacag acaaagggaa
     1201 aataacaaag gcggattgat ggagtggtgg ctaatgatat cggataaact cataacgctg
     1261 gcgaagatcc tctgtgtaat cgtcggcatt tcatttttag tcattctggt tgccattttc
     1321 ttttccaccg cttggcgagt cctgacgtta tcgggactgg tggggtgaaa gagagatgaa
     1381 ccgtgttctg tgtgtggtga ttattgtcct gctggtagcc tgtggtgcac ttagtctggg
     1441 gctgaatcat taccgcgata acgccataac ctacaaagag cagcgcgata aaaaagtcag
     1501 tgagctggag ctggcaaatg caaccattac tgatatgcag cagcgccagc gtgatgttgc
     1561 tgcacttgat gccagatact cgagggaatt agccgatgcg agagctgaaa atgaaactct
     1621 gcgtgctgat gttgccgctg gtcgtaagcg cctgcgcatc aacgccaact gtccaggctc
     1681 cttgcgtaaa gcccccatca cctccggcgt ggataatgca accggtcccc gactggcaga
     1741 agccgctgaa cgggat
//
LOCUS       DAZKHF020000458         1749 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2201S31241
            SAMN43267800-rid25106413.denovo.461, whole genome shotgun sequence.
ACCESSION   DAZKHF020000458 DAZKHF020000000
VERSION     DAZKHF020000458.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267800
            Sequence Read Archive: SRR30307263
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1749)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1749)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 1749)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350581.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350581.2
            Genome Coverage       :: 151x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:21:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,652
            CDSs (total)                      :: 5,557
            Genes (coding)                    :: 5,320
            CDSs (with protein)               :: 5,320
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 237
            CDSs (without protein)            :: 237
            Pseudo Genes (ambiguous residues) :: 0 of 237
            Pseudo Genes (frameshifted)       :: 102 of 237
            Pseudo Genes (incomplete)         :: 130 of 237
            Pseudo Genes (internal stop)      :: 52 of 237
            Pseudo Genes (multiple problems)  :: 41 of 237
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1749
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267800-rid25106413.denovo.461"
                     /serotype="O 26:H11"
                     /isolate="2201S31241"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-01"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            <1..998
                     /locus_tag="ACHOSC_RS24670"
                     /old_locus_tag="pgaptmp_004934"
     CDS             <1..998
                     /locus_tag="ACHOSC_RS24670"
                     /old_locus_tag="pgaptmp_004934"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001480735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015473 - fimbrial usher porin activity
                     [Evidence IEA];
                     GO_process: GO:0009297 - pilus assembly [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="fimbria/pilus outer membrane usher protein"
                     /protein_id="HGU4550689.1"
                     /translation="SLRLNTYVSLARNTYWDASSNVNYSLSLSRDFDIGPLKNVSTSL
                     TFSRINWEEDNQDQLYLNISIPWGTSRTLSYGMQRNQDNKISHTASWYDSSDRNNSWS
                     VSASGDNDEFKDMKASLRASYQHNTENGRLYLSGTSQRDSYYSLNASWNGSFTATRHG
                     AAFHDYSGSADSRFMIDADGAEDIPLNNKRAVTNRYGIGVIPSVSSYITTSLSVDTRN
                     LPENVDIENSVITTTLTEGAIGYAKLDTRKGYQIMGVIRLADGSHPPLGISVKDKTSH
                     KELGLVADGGFVYLNGIQDDSKLTLRWGDKSCFIQPPNSSNLTTGTVILPCISQN"
     gene            1014..>1749
                     /locus_tag="ACHOSC_RS24675"
                     /old_locus_tag="pgaptmp_004935"
     CDS             1014..>1749
                     /locus_tag="ACHOSC_RS24675"
                     /old_locus_tag="pgaptmp_004935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417519.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0061077 - chaperone-mediated protein
                     folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone"
                     /protein_id="HGU4550690.1"
                     /translation="MRYLNTKNLIAAGVLLACMSSIAWGAIIPDRTRIIMNESDKGEA
                     LKLTNQSKNLPYLAQTWIEDTKGNKSRDFIVTVPPMVRLNPSEQIQIRMISQEKIAQL
                     PNDRETLFYFNVREIPPKTDKKNVMQVTMQHALKLFWRPKAIELEDDGVMSYEKVEII
                     RRNDGSIRFNNKMPYHVTLGYIGTNGITMLPQTQSLMVTPYSHADTQFKNVPSAFQVG
                     YINDFGGLSFYEINCPTVNNSCNVSVA"
BASE COUNT          559 a          369 c          362 g          459 t
ORIGIN      
        1 aatcattaag gttaaataca tacgtaagtt tggctcgtaa tacttactgg gacgccagca
       61 gtaatgtgaa ttattcatta tcacttagcc gcgattttga tatcgggcca ttaaaaaacg
      121 tctccacttc tctaacattt agccgaataa actgggaaga agacaaccag gatcaactgt
      181 acctaaatat ttcgattccc tggggaacca gtagaacatt gagctatggt atgcaacgaa
      241 atcaggataa caagatttcg catactgctt cgtggtatga ctcttccgat cgaaataatt
      301 cctggagcgt ttctgcttca ggcgacaatg atgaattcaa agatatgaaa gcgtcactac
      361 gcgccagtta tcagcataat accgagaacg gtcgtctcta tctctccggg acatcacagc
      421 gagacagtta ttattctctg aatgccagtt ggaatggttc attcacggcg actcgtcacg
      481 gagccgcttt ccatgactat agtggtagtg ctgactcgcg ttttatgatc gacgcagatg
      541 gtgcagaaga tattccgttg aacaataaac gcgcggtaac taatcggtat ggcatcggag
      601 ttattccatc agtcagcagt tacataacaa catcattaag cgttgatacc cgaaatcttc
      661 cagaaaatgt ggatatcgaa aactcggtta tcaccaccac cttaaccgaa ggtgctattg
      721 gctacgccaa acttgatacc cgcaagggct atcaaatcat gggggttatt cgcctggcag
      781 atggtagtca cccaccactg gggattagcg taaaagataa aaccagccat aaagaattag
      841 gactggttgc tgatggcggc tttgtatacc tcaacggcat tcaggatgac agcaaactta
      901 ctttacgctg gggtgataaa tcctgtttta ttcaaccacc caatagcagc aacttaacca
      961 ccggaacggt tattttaccg tgtattagcc aaaattaatt caggatgaaa ctaatgcgct
     1021 acttaaacac aaagaatctc attgcagcag gagtattact cgcctgcatg agcagcattg
     1081 cctggggtgc catcatcccg gaccgtaccc gtatcataat gaatgagtca gataaagggg
     1141 aagcattaaa acttactaat caaagcaaaa atctacccta tctggcgcaa acatggatag
     1201 aagatacgaa aggcaataaa tcacgtgact ttattgtcac ggttccccct atggtacgtt
     1261 taaatcccag tgagcaaatt caaataagaa tgataagtca ggaaaaaatt gctcaactgc
     1321 ctaatgacag agaaacgctc ttctatttta acgtgcgaga aataccgcca aaaacagata
     1381 aaaaaaatgt gatgcaagtc actatgcagc acgcattgaa actattctgg cgaccaaaag
     1441 ctattgaatt agaagatgat ggggtaatgt cttacgaaaa agttgaaatc atccgtagaa
     1501 atgatggttc gatccgcttt aacaataaga tgccttatca cgttacgctg ggttatatcg
     1561 gaacaaacgg aatcacaatg ttgccacaaa cgcaaagttt aatggtaacc ccttatagcc
     1621 atgcagatac gcagtttaaa aatgttccgt cagcttttca ggttggttat atcaatgatt
     1681 ttggtggatt aagtttttat gaaataaact gcccgacggt taataactct tgtaatgttt
     1741 ccgtagcca
//
LOCUS       DAZKHF020000459         1743 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2201S31241
            SAMN43267800-rid25106413.denovo.462, whole genome shotgun sequence.
ACCESSION   DAZKHF020000459 DAZKHF020000000
VERSION     DAZKHF020000459.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267800
            Sequence Read Archive: SRR30307263
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1743)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1743)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 1743)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350581.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350581.2
            Genome Coverage       :: 151x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:21:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,652
            CDSs (total)                      :: 5,557
            Genes (coding)                    :: 5,320
            CDSs (with protein)               :: 5,320
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 237
            CDSs (without protein)            :: 237
            Pseudo Genes (ambiguous residues) :: 0 of 237
            Pseudo Genes (frameshifted)       :: 102 of 237
            Pseudo Genes (incomplete)         :: 130 of 237
            Pseudo Genes (internal stop)      :: 52 of 237
            Pseudo Genes (multiple problems)  :: 41 of 237
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1743
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267800-rid25106413.denovo.462"
                     /serotype="O 26:H11"
                     /isolate="2201S31241"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-01"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            <1..663
                     /locus_tag="ACHOSC_RS24680"
                     /old_locus_tag="pgaptmp_004936"
     CDS             <1..663
                     /locus_tag="ACHOSC_RS24680"
                     /old_locus_tag="pgaptmp_004936"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="portal protein"
                     /protein_id="HGU4550691.1"
                     /translation="DGRDYDPDVLQKAVNDAVANIPQPADGKSLTPDDVRPMLEQMVK
                     EAVSHIHVPRDGRDYDPDVLQKAVLDAVSALPAPQDGRDATALEILPAIDDQKSFPRG
                     TYATHQGGLWRAYEKTHGMRGWECLVDGVADIDVSMTGERLFSVVVRQSSGQRTEKTF
                     SLPVMLYRGVFRAGETYHPGDTVTWGGSLWHCNSMTEDKPGEAHSSAWTLAAKRGRDA
                     GG"
     gene            666..992
                     /locus_tag="ACHOSC_RS24685"
                     /old_locus_tag="pgaptmp_004937"
     CDS             666..992
                     /locus_tag="ACHOSC_RS24685"
                     /old_locus_tag="pgaptmp_004937"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0016032 - viral process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="head-tail connector protein"
                     /protein_id="HGU4550692.1"
                     /translation="MTALLTLEEIKAHLRVDHDADDDMLMDKVRQATAVLLAYIQGSR
                     DKVIREDGELIPGEALTRMKGAAMRLTGMLYRNPDLAEREELLQGELPFSVSVLIYDL
                     RCPTVL"
     gene            1002..1352
                     /locus_tag="ACHOSC_RS24690"
                     /old_locus_tag="pgaptmp_004938"
     CDS             1002..1352
                     /locus_tag="ACHOSC_RS24690"
                     /old_locus_tag="pgaptmp_004938"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage head closure protein"
                     /protein_id="HGU4550693.1"
                     /translation="MAISAGRLTQMISVLNPVLTRNAAGEMTEEWVSCGKIHADIRGR
                     SSRERMQSGAEMAQAEIRIWVRGQSGREITAASRLHVLSGPWRDRILNVVGLPVPDAT
                     GGRLEILCRLGGEK"
     gene            1349..>1743
                     /locus_tag="ACHOSC_RS24695"
                     /old_locus_tag="pgaptmp_004939"
     CDS             1349..>1743
                     /locus_tag="ACHOSC_RS24695"
                     /old_locus_tag="pgaptmp_004939"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HK97-gp10 family putative phage morphogenesis
                     protein"
                     /protein_id="HGU4550694.1"
                     /translation="MIETLLDFSGLEDISRDLQLLSGAENNRVLREATRAGANVLKEE
                     VVSRAPVRRGKLRRNVVVLSRRSRDGGMESGVHIRGVNPDTGNSDNTMKADNPRNAFY
                     WRFVEMGTVNMPPHPFVRPAFDVRSEQAA"
BASE COUNT          373 a          420 c          597 g          353 t
ORIGIN      
        1 gacggtcgtg actatgatcc cgatgttctg cagaaggcgg tgaatgatgc ggtcgcaaat
       61 attccgcagc cggcagacgg taaaagtctc accccggatg atgtgcgtcc gatgcttgaa
      121 cagatggtga aggaggcggt aagccatatt catgttccgc gtgatggtcg tgactacgat
      181 ccggatgttc tgcagaaggc ggttctggat gcggtgagtg ccctgccggc tccgcaggac
      241 gggcgtgatg ccacggctct ggaaatactc cccgccattg acgatcaaaa atcctttccc
      301 cggggcacgt atgccacaca ccagggcgga ctctggcggg cgtatgaaaa aacgcacggg
      361 atgcggggat gggaatgcct ggttgacggg gtggcggata ttgacgtcag catgacgggt
      421 gagcggttgt tctctgtggt ggtccggcag agcagtggcc agcgtacgga aaaaacattt
      481 tccctgccgg tgatgctcta ccgcggtgtg ttcagagccg gtgaaaccta ccaccccggc
      541 gatacggtga cgtggggggg ctcgctgtgg cactgcaaca gtatgaccga agataaaccc
      601 ggagaagctc attcatcagc ctggaccctg gctgcaaaac gtgggcggga tgcaggaggc
      661 tgaaaatgac ggcattactg acactggaag agatcaaggc acatctgcgt gtcgaccatg
      721 acgcggatga tgacatgctg atggacaagg ttcgtcaggc taccgccgtg ctgctggcct
      781 acattcaggg cagccgggat aaagtgatcc gtgaggacgg tgaactgatc ccgggcgagg
      841 cattaacccg gatgaagggg gctgccatgc gactgaccgg gatgctgtac cggaatccgg
      901 atcttgcgga gcgggaagaa ctgcttcagg gggagctgcc gttttctgtt tccgtgctga
      961 tttacgattt gcgttgtccg acggtgttat gaggaggggg aatggcaata tctgcaggtc
     1021 gtctgacaca gatgataagt gttctgaacc cggtgttaac ccgtaacgct gccggagaaa
     1081 tgacggaaga atgggtgtca tgcgggaaaa ttcatgcgga tatccgtggc aggagcagcc
     1141 gggagcggat gcagtctggt gcggaaatgg cgcaggcgga aatccgcatc tgggtgcgcg
     1201 gtcagtccgg tcgggaaatc acggcagcgt cacgacttca tgtgctgagt ggtccctggc
     1261 gtgaccggat cctgaacgtt gtcgggctgc ccgtgccgga tgcgaccggc ggacgtctgg
     1321 aaattctctg tcggctggga ggtgaaaaat gatcgaaacc ctgctggatt tttcggggct
     1381 ggaggacatc agccgcgatt tgcagcttct gagtggtgca gaaaataacc gggtgctgcg
     1441 tgaggcaacc cgtgcgggtg cgaatgtgct gaaagaagaa gtggtgtcac gggcaccggt
     1501 acgcagggga aaactgcgcc gcaatgtggt ggtcctttcc cggcgctccc gcgatggcgg
     1561 gatggaatcc ggtgtgcata tccggggtgt taatccggac accggtaaca gcgataacac
     1621 catgaaggcg gataacccgc gcaacgcttt ctactggcgg tttgtggaaa tggggaccgt
     1681 gaatatgcca ccgcacccgt ttgtgcgtcc ggcgtttgat gtgcgcagtg aacaggcagc
     1741 gca
//
LOCUS       DAZKHF020000460         1742 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2201S31241
            SAMN43267800-rid25106413.denovo.463, whole genome shotgun sequence.
ACCESSION   DAZKHF020000460 DAZKHF020000000
VERSION     DAZKHF020000460.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267800
            Sequence Read Archive: SRR30307263
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1742)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1742)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 1742)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350581.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350581.2
            Genome Coverage       :: 151x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:21:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,652
            CDSs (total)                      :: 5,557
            Genes (coding)                    :: 5,320
            CDSs (with protein)               :: 5,320
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 237
            CDSs (without protein)            :: 237
            Pseudo Genes (ambiguous residues) :: 0 of 237
            Pseudo Genes (frameshifted)       :: 102 of 237
            Pseudo Genes (incomplete)         :: 130 of 237
            Pseudo Genes (internal stop)      :: 52 of 237
            Pseudo Genes (multiple problems)  :: 41 of 237
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1742
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267800-rid25106413.denovo.463"
                     /serotype="O 26:H11"
                     /isolate="2201S31241"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-01"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            6..275
                     /locus_tag="ACHOSC_RS24700"
                     /old_locus_tag="pgaptmp_004940"
     CDS             6..275
                     /locus_tag="ACHOSC_RS24700"
                     /old_locus_tag="pgaptmp_004940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310967.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail fiber C-terminal domain-containing
                     protein"
                     /protein_id="HGU4550695.1"
                     /translation="MNILKKLMQRMCGCGKHDGREHVQSLTAQLRLGPADILESDENG
                     IIPEQDRVITQVVILDADKKQIQCVVRPLQILRGDGTWENIGGMK"
     gene            complement(392..667)
                     /locus_tag="ACHOSC_RS24705"
                     /old_locus_tag="pgaptmp_004941"
     CDS             complement(392..667)
                     /locus_tag="ACHOSC_RS24705"
                     /old_locus_tag="pgaptmp_004941"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001443842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="secretion protein EspO"
                     /protein_id="HGU4550696.1"
                     /translation="MPFSIKNIFSNSKGSYPEISGPVQDKPVSKNCTLTSTTCSMNDY
                     TVFSRKSCTFDMRPPGAGDRTPQLKLSASELIWLSKTIDTERNNIKE"
     gene            complement(730..>1742)
                     /locus_tag="ACHOSC_RS24710"
                     /old_locus_tag="pgaptmp_004942"
     CDS             complement(730..>1742)
                     /locus_tag="ACHOSC_RS24710"
                     /old_locus_tag="pgaptmp_004942"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="type III secretion protein GogB"
                     /protein_id="HGU4550697.1"
                     /translation="EGIECLTVCRCHISGVPESVRYLEIKGSATDSIKNVPNGLSSLS
                     INSYNPENQARIDNLISPSLQTLSLTGCSNIILPEILPESVTSVTIHAEQKTTWNIGV
                     EGMPDGLDLDLQNVLLSPDVVKAKNITFQGNALDVALHFREGDIVYGLSSPREKLVNS
                     IKLVNDFSKKDIITQNTLTNAVWDPRTPRKYKQDPLIKRALNEHERGIKFKQHLKSHN
                     NYNVTMADLSVYNRDKLWAKTSKAGLEFQTLTRNKTVIFCADEIVNSLKLIANKSDGY
                     GQSITASELRWIYRNKDNDQIMKNIKFYLRGKEIPAEKILGAPEWKDYKPKYSGATYK
                     YS"
BASE COUNT          402 a          345 c          415 g          580 t
ORIGIN      
        1 atgacatgaa catactaaaa aaacttatgc agcgtatgtg tggttgcgga aagcatgatg
       61 gccgtgaaca cgtgcagtcg cttacagcac agctgcgact gggaccggca gacattctgg
      121 agtccgatga gaatggcatt atcccggagc aggacagggt aatcacgcag gtggtgatac
      181 tggatgcgga taaaaagcag atacagtgcg tggtaagacc gctgcaaatt ctgcgtggtg
      241 acgggacgtg ggaaaatatt ggcgggatga agtaactcga cagcttcaca aaaccggagt
      301 ccggctccat tttaccacct ccacgggagg tggttgcttg caatcagatg tttcgggcct
      361 gcggcttttt gtgttcccta gtaagtggtt actattcttt tatgttattc ctctctgtat
      421 ctattgtctt agatagccat atgagttcag atgctgagag ttttaattgt ggtgttctgt
      481 ctcctgctcc gggtggacgc atgtcaaaag tgcaggattt tctgctgaat actgtgtagt
      541 cattcataga gcatgttgtt gaggttagtg tacaattctt cgatactggc ttgtcctgca
      601 caggtccgga tatttctggg tagcttcctt ttgaatttga aaatatgttt ttgattgaaa
      661 atggcatcgt gctttgtcct ttgaactacg atatattgtt ttgtgttctt tcgttgtgag
      721 taggtatcat taagaatact tatatgtggc tccagagtat ttgggtttgt agtctttcca
      781 ctctggtgcg cctaatattt tttctgctgg tatctctttc ccacgcaagt aaaactttat
      841 gtttttcatt atctggtcgt tgtctttgtt gcgataaatc catcgtaatt cgctggcagt
      901 aatactctga ccataaccat ctgacttatt agcaatgagt ttgagtgagt tgacaatctc
      961 gtcagcacaa aaaataaccg ttttgttgcg tgttaatgtc tgaaactcca ggccggcctt
     1021 gcttgttttt gcccataatt tgtcgcgatt gtatacggaa aggtcggcca tggtgacatt
     1081 ataattattg tggctcttta ggtgttgttt aaattttatc cctctttcgt gttcatttaa
     1141 tgctcttttg atcagtggat cttgcttata tttgcgaggt gttctggggt cccatactgc
     1201 gtttgttaac gtattctgag ttataatatc ttttttggaa aagtcgttaa ctagtttaat
     1261 gctgttgaca agtttttctc tgggtgaaga tagtccatag acaatgtctc cctcgcgaaa
     1321 gtgtaaggcc acatccagag cgttgccctg aaaggtgatg ttttttgctt taactacatc
     1381 tggagagagt agtacatttt gtaaatcaag atccagccca tcaggcatcc cttcaacacc
     1441 gatgttccac gtggttttct gctccgcatg aatggttacc gatgtcacac tctccggaag
     1501 tatctccggc agtataatat tgctacatcc agtcagcgat agcgtctgca gtgacggtga
     1561 tatcaggttg tcaattctgg cctgattctc cgggttatag ctattgatgc tgagagatga
     1621 taacccgttt ggaacatttt ttatgctgtc tgtggcgcta ccttttatct caaggtagcg
     1681 gacgctctct ggcactccag atatatggca gcgacaaacc gtcaggcatt cgatgccttc
     1741 ag
//
LOCUS       DAZKHF020000461         1727 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2201S31241
            SAMN43267800-rid25106413.denovo.464, whole genome shotgun sequence.
ACCESSION   DAZKHF020000461 DAZKHF020000000
VERSION     DAZKHF020000461.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267800
            Sequence Read Archive: SRR30307263
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1727)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1727)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 1727)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350581.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350581.2
            Genome Coverage       :: 151x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:21:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,652
            CDSs (total)                      :: 5,557
            Genes (coding)                    :: 5,320
            CDSs (with protein)               :: 5,320
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 237
            CDSs (without protein)            :: 237
            Pseudo Genes (ambiguous residues) :: 0 of 237
            Pseudo Genes (frameshifted)       :: 102 of 237
            Pseudo Genes (incomplete)         :: 130 of 237
            Pseudo Genes (internal stop)      :: 52 of 237
            Pseudo Genes (multiple problems)  :: 41 of 237
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1727
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267800-rid25106413.denovo.464"
                     /serotype="O 26:H11"
                     /isolate="2201S31241"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-01"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            <1..395
                     /gene="cmtB"
                     /locus_tag="ACHOSC_RS24715"
                     /old_locus_tag="pgaptmp_004943"
     CDS             <1..395
                     /gene="cmtB"
                     /locus_tag="ACHOSC_RS24715"
                     /old_locus_tag="pgaptmp_004943"
                     /EC_number="2.7.1.197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="PTS mannitol transporter subunit IIA"
                     /protein_id="HGU4550698.1"
                     /translation="AKDWQEAIDFSMVSLLDKNYISENYIQAIKDSTINNGPYYILAP
                     GVAMPHARPECGALKTGMSLTLLEQGVYFPGNDEPIKLLIGLSAADADSHIGAIQALS
                     ELLCEEEILEQLLTASSEKQLADIISRG"
     gene            423..>1727
                     /gene="cmtA"
                     /locus_tag="ACHOSC_RS24720"
                     /old_locus_tag="pgaptmp_004944"
     CDS             423..>1727
                     /gene="cmtA"
                     /locus_tag="ACHOSC_RS24720"
                     /old_locus_tag="pgaptmp_004944"
                     /EC_number="2.7.1.197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannitol transporter subunit IICB"
                     /protein_id="HGU4550699.1"
                     /translation="MENKSARAKVQAFGGFLTAMVIPNIGAFIAWGFITALFIPTGWL
                     PNEHFAKIVGPMITYLLPVMIGSTGGHLVGGKRGAVMGGIGTIGVIVGAEIPMFLGSM
                     IMGPLGGLVIKYVDKALEKRIPAGFEMVINNFSLGIAGMLLCLLGFEVIGPAVLIANT
                     FVKECIEALVHAGYLPLLSVINEPAKVLFLNNAIDQGVYYPLGMQQASVNGKSIFFMV
                     ASNPGPGLGLLLAFTLFGKGMSKRSAPGAMIIHFLGGIHELYFPYVLMKPLTIIAMIA
                     GGMSGTWMFNLLDGGLVAGPSPGSIFAYLALTPKGSFLATIAGVTVGTLVSFAITSLI
                     LKMEKTVETESEDEFAQSANAVKAMKQEGAFSLSRVKRIAFVCDAGMGSSAMGATTFR
                     KRLEKAGLAIEVKHYAIENVPADADIVVTHASLEGRVKRVTDK"
BASE COUNT          407 a          377 c          470 g          473 t
ORIGIN      
        1 cagcaaaaga ttggcaggaa gctatcgatt tctcgatggt atcattgctg gataaaaact
       61 atatcagcga gaattacatt caggcaatta aagattccac cattaacaat ggcccttatt
      121 atattctcgc accaggcgtg gcaatgcctc atgcgcgacc ggaatgtggg gcgcttaaaa
      181 ccgggatgtc tttgacatta cttgaacaag gtgtttattt tccggggaat gacgaaccaa
      241 ttaaattact catcggactc tctgctgccg atgccgattc gcacattggc gctattcagg
      301 cgttaagtga attactgtgc gaagaagaaa tactcgaaca actcttaaca gcatcatcag
      361 aaaaacaatt agcggacatt atcagccgcg gataattact ctctcctcta caggataata
      421 ttatggaaaa caagtctgct cgtgcaaagg tccaggcttt tgggggcttt ttgactgcaa
      481 tggtcatccc caatattggt gcttttattg cctggggttt tattactgcg ttatttattc
      541 ccaccggttg gctgcctaac gaacatttcg ccaaaattgt cggcccgatg attacctatt
      601 tattgcccgt gatgattggt tctacaggtg gtcatctggt cggcggtaaa cgcggggcgg
      661 tcatgggcgg aataggtact attggtgtga tcgttggcgc agagatcccg atgttccttg
      721 gctcaatgat catggggccg ctcggtgggt tggtcataaa atatgtcgat aaggcactgg
      781 aaaaacgcat acctgccggt tttgagatgg ttatcaataa cttctcatta ggtatcgcgg
      841 ggatgctcct ttgtctgctg ggttttgaag tgatcggccc ggcggtgtta attgccaata
      901 ctttcgtcaa agagtgtatc gaggcgctgg tacatgcggg ttatctgcct ctgctgtcag
      961 tcatcaatga accggcgaaa gtgcttttcc ttaataatgc gatcgatcag ggcgtctatt
     1021 atccgctggg aatgcaacag gcttcggtta acggtaaatc catcttcttt atggtggcct
     1081 ctaacccagg tccgggcctg gggctgctgc tggcgtttac cttgtttggt aaagggatga
     1141 gtaaacgttc tgcgcccggg gcgatgatta ttcacttcct cggtgggatc cacgaactgt
     1201 atttcccata tgtgctgatg aagccgctga ctattattgc catgattgcg ggcggtatgt
     1261 ctggtacctg gatgtttaac ttactggacg gtggtctggt ggctggccca agtccggggt
     1321 ctatctttgc ttacctggca ctgacgccga aaggctcgtt cctggcgaca attgccggtg
     1381 ttacggtagg taccctggtg tcctttgcta ttacttcgct gatactgaag atggaaaaaa
     1441 cggtggaaac ggagagcgaa gatgagtttg ctcagtcagc caatgcggtt aaggcgatga
     1501 aacaagaggg tgcattctcg ttaagcaggg ttaagcgtat cgcctttgtt tgcgatgcgg
     1561 ggatgggctc cagtgcgatg ggcgcgacca ccttccgtaa acgcctggaa aaagcggggc
     1621 tggcaataga agtaaaacat tacgcgatag aaaacgtgcc tgcggatgcc gatatcgtcg
     1681 tcactcacgc cagtctggaa gggcgcgtga aacgtgtgac ggataaa
//
LOCUS       DAZKHF020000462         1726 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2201S31241
            SAMN43267800-rid25106413.denovo.465, whole genome shotgun sequence.
ACCESSION   DAZKHF020000462 DAZKHF020000000
VERSION     DAZKHF020000462.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267800
            Sequence Read Archive: SRR30307263
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1726)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1726)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 1726)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350581.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350581.2
            Genome Coverage       :: 151x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:21:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,652
            CDSs (total)                      :: 5,557
            Genes (coding)                    :: 5,320
            CDSs (with protein)               :: 5,320
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 237
            CDSs (without protein)            :: 237
            Pseudo Genes (ambiguous residues) :: 0 of 237
            Pseudo Genes (frameshifted)       :: 102 of 237
            Pseudo Genes (incomplete)         :: 130 of 237
            Pseudo Genes (internal stop)      :: 52 of 237
            Pseudo Genes (multiple problems)  :: 41 of 237
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1726
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267800-rid25106413.denovo.465"
                     /serotype="O 26:H11"
                     /isolate="2201S31241"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-01"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            complement(384..1322)
                     /gene="lrhA"
                     /locus_tag="ACHOSC_RS24725"
                     /old_locus_tag="pgaptmp_004945"
     CDS             complement(384..1322)
                     /gene="lrhA"
                     /locus_tag="ACHOSC_RS24725"
                     /old_locus_tag="pgaptmp_004945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator LrhA"
                     /protein_id="HGU4550700.1"
                     /translation="MISANRPIINLDLDLLRTFVAVADLNTFAAAAAAVCRTQSAVSQ
                     QMQRLEQLVGKELFARHGRNKLLTEHGIQLLGYARKILRFNDEACSSLMFSNLQGVLT
                     IGASDESADTILPFLLNRVSSVYPKLALDVRVKRNAYMAEMLESQEVDLMVTTHRPSA
                     FKALNLRTSPTHWYCAAEYVLQKGEPIPLVLLDDPSPFRDMVLATLNKADIPWRLAYV
                     ASTLPAVRAAVKAGLGVTARPVEMMSPDLRVLSGVDGLPPLPDTEYLLCYDPSSNNEL
                     AQVIYQAMESYHNPWQYSPMSAPEGDDSLLIERDIE"
BASE COUNT          469 a          406 c          384 g          467 t
ORIGIN      
        1 ctccaggata attagatgtt gttgaatcgt gtccgttgtg aagcaatgga aaaaatacgg
       61 gtctattttg acaggaattt gtgtcgattc ctccccccaa aagagagtat tttcttgatc
      121 tgtgacacgc ttttgtcatt ccataacaaa aacgcagcaa caaatttacg tattttttaa
      181 catcattgta gcaggtgatt tttttcaggc gattatttgt gcgttcggga cgtgaatctc
      241 tggtgattga aaaatgaaca gttttgtacg ttctgcacta tgcgaggaag gatttttact
      301 aaaaaaaagc cgctggggtt taaaacaccc ccagcggctc gttttttaca ctattgtctc
      361 aggaattatc tatcgtccgt cgattactcg atatcccttt caatcaacaa tgaatcatcc
      421 ccttccggag cagacattgg gctgtattgc cacggattat ggtagctctc catcgcctga
      481 tagatcacct gggccagctc attattactg gacgggtcat agcacagcaa atattcggta
      541 tcaggtagcg gcggtaagcc atctactcca ctcagcacac gcaggtccgg gctcatcatc
      601 tcaaccggcc ttgccgtcac gccaagaccg gctttcactg ccgcacgaac ggccggaagc
      661 gtcgaggcga cataagccag tcgccatgga atatctgctt tattaagcgt cgccagcacc
      721 atatcgcgaa acggacttgg atcatccagc aatacaagag ggatcggctc gcctttttgc
      781 aatacgtatt ccgctgcgca gtaccagtgt gttggcgagg ttcgcaggtt caatgcctta
      841 aacgctgaag gacgatgggt ggttaccatc aaatccactt cctgggattc cagcatttct
      901 gccatatagg cattacgttt aacgcggaca tccagcgcta atttcggata aaccgaactc
      961 acgcgattta acaggaaagg taagatcgta tcggcagatt catctgaagc accgatagtt
     1021 aacacgccct gaagattact gaacattaat gatgagcagg cctcatcatt aaaacgcagg
     1081 attttcctgg cgtaaccaag aagttgaatg ccatgttcag ttaacagttt gttgcgaccg
     1141 tgacgagcga acagttcttt cccaacgagt tgttccagac gctgcatttg ctgacttacg
     1201 gcggactgag tacgacacac agcggcagct gcggcagcaa aagtgttcag atcggcaaca
     1261 gcaacaaatg ttctcagcag atcgaggtcg aggttaatta tcggacgatt tgcacttatc
     1321 atatattatc acttactggc ggctcatact gagctggtta atgctgtgca cacacaaaca
     1381 agcaattcca tttgtaatgt gcctccctgg cagtttcatc cggaacccgg acgaaagtaa
     1441 aaatgcatat gagttgcact aaaaaagcga ctcacattgt tccgttataa tgcctgaagt
     1501 agatcacaga atatatcttc agggatcgca tatctattaa gttactcact cttttctatt
     1561 tatgacatgc gcgtgtttgt ataaatgtaa atgtgagtcc ttgttccact ctcgtgcagc
     1621 atcgctggtc atacgcgaac acgtacaaac agcaatggtg aggcggcatc aagagcaggg
     1681 atccgtcatt tatgcgagca ttttacccca aaaacctttt atttat
//
LOCUS       DAZKHF020000463         1720 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2201S31241
            SAMN43267800-rid25106413.denovo.466, whole genome shotgun sequence.
ACCESSION   DAZKHF020000463 DAZKHF020000000
VERSION     DAZKHF020000463.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267800
            Sequence Read Archive: SRR30307263
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1720)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1720)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 1720)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350581.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350581.2
            Genome Coverage       :: 151x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:21:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,652
            CDSs (total)                      :: 5,557
            Genes (coding)                    :: 5,320
            CDSs (with protein)               :: 5,320
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 237
            CDSs (without protein)            :: 237
            Pseudo Genes (ambiguous residues) :: 0 of 237
            Pseudo Genes (frameshifted)       :: 102 of 237
            Pseudo Genes (incomplete)         :: 130 of 237
            Pseudo Genes (internal stop)      :: 52 of 237
            Pseudo Genes (multiple problems)  :: 41 of 237
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1720
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267800-rid25106413.denovo.466"
                     /serotype="O 26:H11"
                     /isolate="2201S31241"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-01"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            complement(102..545)
                     /locus_tag="ACHOSC_RS24730"
                     /old_locus_tag="pgaptmp_004946"
     CDS             complement(102..545)
                     /locus_tag="ACHOSC_RS24730"
                     /old_locus_tag="pgaptmp_004946"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyltransferase"
                     /protein_id="HGU4550701.1"
                     /translation="MVISIRRSQHEEGEELVAIWCRSVDATHDFLSAEYRAELEELVR
                     SFLPEAPLWVAVNERDQPVGFMLLSGQHMDALFIDPDVRGCGVGRMLVEHALSMAPEL
                     TTNVNEQNEQAVGFYKKVGFKVTGRSEVDDLGKPYPLLNLAYVGE"
     gene            702..1631
                     /gene="metA"
                     /locus_tag="ACHOSC_RS24735"
                     /old_locus_tag="pgaptmp_004947"
     CDS             702..1631
                     /gene="metA"
                     /locus_tag="ACHOSC_RS24735"
                     /old_locus_tag="pgaptmp_004947"
                     /EC_number="2.3.1.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008899 - homoserine O-succinyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0019281 - L-methionine biosynthetic process
                     from homoserine via O-succinyl-L-homoserine and
                     cystathionine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine O-succinyltransferase"
                     /protein_id="HGU4550702.1"
                     /translation="MPIRVPDELPAVNFLREENVFVMTTSRASGQEIRPLKVLILNLM
                     PKKIETENQFLRLLSNSPLQVDIQLLRIDSRESRNTPAEHLNNFYCNFEDIQEQNFDG
                     LIVTGAPLGLVEFNDVAYWPQIKQVLEWSKDHVTSTLFVCWAVQAALNILYGIPKQTR
                     TDKLSGVYEHHILHPHALLTRGFDDSFLAPHSRYADFPAALIRDYTDLEILAETEEGD
                     AYLFASKDKRIAFVTGHPEYDAQTLAQEYFRDVEAGLGPEVPYNYFPHNDPQNTPRAS
                     WRSHGNLLFTNWLNYYVYQITPYDLRHMNPTLD"
BASE COUNT          414 a          481 c          388 g          437 t
ORIGIN      
        1 aaaggccatc agtcacgatg gcctttttgc gttggtgaaa aaaatgccgg gtgcgacgct
       61 ggcgcgtctt ctccagcctg ggcaggcacg gcaggggcgg cttattcccc cacatacgcc
      121 agattcagca acggatacgg tttccccaaa tcgtccacct cagagcgtcc cgtaacctta
      181 aaacccacct tcttatagaa cccaaccgcc tgctcatttt gctcattaac gttggttgtc
      241 agttccggtg ccatcgagag cgcatgctcc accagcatcc gacccacgcc gcagccgcgc
      301 acatcaggat cgataaacag cgcatccata tgctgcccac ttaacaacat aaacccaacc
      361 ggctgatccc gttcattaac cgcgacccac aacggtgctt ccggcagaaa ggaacgaacc
      421 agctcttcca gctcggcccg atactctgct gataaaaaat cgtgggtggc atcgacagat
      481 cgacaccaaa tcgcaacgag ttcctcccct tcctcatgct gtgagcggcg aatactaata
      541 accattttct ctccttttcg tcattcttat attctaacgt agccttttcc ttgaaacttt
      601 ctcaccttca acatgcaggc tcgacattgg caaattttct ggttatcttc agctatctgg
      661 atgtctaaac gtataagcgt atgtagtgag gtaatcaggt tatgcctatt cgtgtgccgg
      721 acgagctacc cgccgtcaat ttcttgcgtg aagaaaacgt ctttgtgatg acaacttctc
      781 gtgcgtctgg tcaggaaatt cgtccactta aggttctaat ccttaacctg atgccgaaga
      841 agattgaaac tgaaaatcag tttctgcgcc tgctttcaaa ctcacctttg caggtcgata
      901 ttcagctgtt gcgcatcgat tcccgtgaat cgcgcaacac gcccgcagag catctgaaca
      961 acttctactg taactttgaa gatattcagg aacagaactt tgacggtctg attgtaaccg
     1021 gtgcgccgct gggcctggtg gagtttaatg atgtcgctta ctggccgcag atcaaacagg
     1081 tgctggagtg gtcgaaagat cacgtcacct cgacgctgtt tgtctgctgg gcggtacagg
     1141 ccgcgctcaa tatcctctac ggcattccta agcaaactcg caccgacaaa ctctctggcg
     1201 tttacgagca tcatattctc catcctcatg cgcttctgac gcgtggcttt gatgattcat
     1261 tcctggcacc acattcgcgc tatgctgact ttccggcagc gttgattcgt gattacaccg
     1321 atctggaaat tctggcagag acggaagaag gggatgcata tctgtttgcc agcaaagata
     1381 agcgcattgc ctttgtgacg ggccatcccg aatatgatgc gcaaacgctg gcgcaggaat
     1441 atttccgcga tgtggaagcc ggactaggcc cggaagtacc gtataactat ttcccgcaca
     1501 atgatccgca aaatacaccg cgagcgagct ggcgtagtca cggtaatttg ctgtttacca
     1561 actggctcaa ctattacgtc tatcagatca cgccatacga tctacggcac atgaatccaa
     1621 cgctggatta atcttctgtg atagtcgatc gttaagcgat tcagcacctt acctcaggca
     1681 ccttcgggtg ccttttttat ttccgaaacg cacctcagga
//
LOCUS       DAZKHF020000464         1719 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2201S31241
            SAMN43267800-rid25106413.denovo.467, whole genome shotgun sequence.
ACCESSION   DAZKHF020000464 DAZKHF020000000
VERSION     DAZKHF020000464.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267800
            Sequence Read Archive: SRR30307263
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1719)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1719)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 1719)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350581.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350581.2
            Genome Coverage       :: 151x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:21:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,652
            CDSs (total)                      :: 5,557
            Genes (coding)                    :: 5,320
            CDSs (with protein)               :: 5,320
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 237
            CDSs (without protein)            :: 237
            Pseudo Genes (ambiguous residues) :: 0 of 237
            Pseudo Genes (frameshifted)       :: 102 of 237
            Pseudo Genes (incomplete)         :: 130 of 237
            Pseudo Genes (internal stop)      :: 52 of 237
            Pseudo Genes (multiple problems)  :: 41 of 237
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1719
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267800-rid25106413.denovo.467"
                     /serotype="O 26:H11"
                     /isolate="2201S31241"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-01"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            complement(7..204)
                     /locus_tag="ACHOSC_RS24740"
                     /old_locus_tag="pgaptmp_004948"
     CDS             complement(7..204)
                     /locus_tag="ACHOSC_RS24740"
                     /old_locus_tag="pgaptmp_004948"
                     /inference="COORDINATES: protein motif:HMM:NF024547.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3987 domain-containing protein"
                     /protein_id="HGU4550703.1"
                     /translation="MIGKAIIKAQYNKQVPLTLVAMSVLTAMSIACQNQVDVCSPGNL
                     RGAVNIYTMVLEKGKRPGERY"
     gene            complement(431..727)
                     /locus_tag="ACHOSC_RS24745"
                     /old_locus_tag="pgaptmp_004949"
     CDS             complement(431..727)
                     /locus_tag="ACHOSC_RS24745"
                     /old_locus_tag="pgaptmp_004949"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HGU4550704.1"
                     /translation="MPDQSGHCRTPSRILRMPELSQLLGISRSTIYEKMNPLSKYYDA
                     TFPRPVRLGSGSVGWRSSAIDEWLTLHTVPARSAVKGVNDEVRHTLSSDMTMSR"
     gene            complement(906..1642)
                     /locus_tag="ACHOSC_RS24750"
                     /old_locus_tag="pgaptmp_004950"
                     /pseudo
     CDS             complement(906..1642)
                     /locus_tag="ACHOSC_RS24750"
                     /old_locus_tag="pgaptmp_004950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477181.1"
                     /note="programmed frameshift; internal stop; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS1 family transposase"
BASE COUNT          465 a          366 c          366 g          522 t
ORIGIN      
        1 atttcactaa tacctttccc ccggtctttt ccctttctcc agaaccatag tgtaaatatt
       61 aacggcccca cgtaaatttc cgggactgca tacgtcaacc tgattctgac aggcaattga
      121 catcgctgtt aatactgaca ttgcaaccag agtcagaggt acttgtttat tgtattgagc
      181 tttaattatt gccttcccta tcattgatgg tcatgcatat actgaatacg acatatccgg
      241 tttgatgtaa ggcataaatg cttccttttt cgtaatacgt ttcggttata gcagcaatta
      301 ccgctacaat agcgacaata gctgctcttt caacaatgaa tgctggaaat tctgtttatc
      361 aattaaactt ctggctaaat tgttcttatg cttctttttc ctcctgaggt gatcacgtga
      421 tgacaaaaac ttatctgctc atggtcatat ctgaagaaag cgtatgtctc acttcgtcat
      481 taacaccttt gactgctgat ctggcaggta cagtatgtaa agtaagccat tcgtcaatag
      541 ctgatgaacg ccagcccaca gaaccactac caagacgtac cggcctgggg aaggttgcat
      601 cgtagtattt cgataacgga ttcatttttt cgtaaattgt cgaacgagat atacctaaca
      661 attggctcag ttcaggcatc cgtaagatgc gtgaaggagt cctgcagtgt ccggattgat
      721 ctggcatttt atcccccata gtgtggtgaa ctgtcgttac agtttgtatt ccccgggtaa
      781 atttgtctgc gtaaaaaaat gggttgagaa aaaagacaat aaataattaa aaatcaatat
      841 gatagaaaac gacagcatta gtgttatttt acttttccct tatcgctgtc gattctcatt
      901 atcattcatg gcaaaaaaac agcaaaagag gccctggtaa tgactcctgt cagtagtaca
      961 tgtgttttcg ataaatgaac caatggcttt tcgtgaattt ccagggagcg taaaaagcaa
     1021 atcgttttcc gggcaggtcg tttgatgtga gtacgaagtg tcagattgtt gcgctcaatg
     1081 cgccgggtaa atatcttgcc gacaagatgc ttatcctgtg gcatttctct ggtataactg
     1141 ctccggttgt ctctggttat catgcctgcg gagaatggct tcaggaattc cggcaactca
     1201 cggcatgttt catcagtgcg aggaccaaaa gtgtaagcca gcacaccgtc agctttggtc
     1261 ttatacgcgt accagtgcca ctgctgacga gctttgtttt cgacaaaact ccattgttca
     1321 tcaagttcac agataagtgc cacatcgaca ctggcggctg gagatatggt tactttacgt
     1381 ggtgagcgtt ttttaaagtg agaatgacgg tgttgatatc caccttcagc gttctggagc
     1441 tatcgcgaac cccggctccg ttatgaacca tttcaacaat ttgctcttta atgcctggtt
     1501 ttcgggtttc gtagatgtaa ttcagttgaa atacacgttt gcaggattag cactggaaac
     1561 gctcatgatc tgaggtgctg tgatcataac ggtacacttt ataggaatta cagcggggag
     1621 aatatactgt aactgttgcc atatggtctc cagataccaa tagaatacaa cattaatcta
     1681 tcgtcagaag gcatcaccgg ggccttctga cataatctg
//
LOCUS       DAZKHF020000465         1717 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2201S31241
            SAMN43267800-rid25106413.denovo.468, whole genome shotgun sequence.
ACCESSION   DAZKHF020000465 DAZKHF020000000
VERSION     DAZKHF020000465.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267800
            Sequence Read Archive: SRR30307263
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1717)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1717)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 1717)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350581.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350581.2
            Genome Coverage       :: 151x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:21:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,652
            CDSs (total)                      :: 5,557
            Genes (coding)                    :: 5,320
            CDSs (with protein)               :: 5,320
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 237
            CDSs (without protein)            :: 237
            Pseudo Genes (ambiguous residues) :: 0 of 237
            Pseudo Genes (frameshifted)       :: 102 of 237
            Pseudo Genes (incomplete)         :: 130 of 237
            Pseudo Genes (internal stop)      :: 52 of 237
            Pseudo Genes (multiple problems)  :: 41 of 237
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1717
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267800-rid25106413.denovo.468"
                     /serotype="O 26:H11"
                     /isolate="2201S31241"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-01"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            complement(61..1599)
                     /gene="cadC"
                     /locus_tag="ACHOSC_RS24755"
                     /old_locus_tag="pgaptmp_004951"
     CDS             complement(61..1599)
                     /gene="cadC"
                     /locus_tag="ACHOSC_RS24755"
                     /old_locus_tag="pgaptmp_004951"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine decarboxylation/transport transcriptional
                     activator CadC"
                     /protein_id="HGU4550705.1"
                     /translation="MQQPVVRVGEWLVTPSINQISRNGRQLTLEPRLIDLLVFFAQHS
                     GEVLSRDELIDNVWKRSIVTNHVVTQSISELRKSLKDNDEDSPVYIATVPKRGYKLMV
                     PVIWYSEEEGEEIMLSSPPPIPEAVPATDSPSHSLNIQNTATPPEQSPVKSKRFTTFW
                     VWFFFLLSLGICVALVAFSTLDTRLPMSKSRILLNPRDIDINMVNKSCNSWSSPYQLS
                     YAIGVGDLVATSLNTFSTFMVHDKINYNIDEPSSSGKTLSIAFVNQRQYRAQQCFMSI
                     KLVDNADGSTMLDKRYVITNGNQLAIQNDLLESLSKALNQPWPQRMQETLQQILPHRG
                     ALLTNFYQAHDYLLHGDDKSLNRASELLGEIVQSSPEFTYARAEKALVDIVRHSQHPL
                     DEKQLAALNTEIDNIVTLPELNNLSIIYQIKAVSALVKGKTDESYQAINTGIDLEMSW
                     LNYVLLGKVYEMKGMNREAADAYLTAFNLRPGANTLYWIENGIFQTSVPYVVPYLDKF
                     LASE"
BASE COUNT          505 a          349 c          357 g          506 t
ORIGIN      
        1 cagaaacata caaaaactca acaacaaata ttcccgagca taaatcaacc cgggagttac
       61 ttattctgaa gcgagaaatt tgtcgagata aggtacaaca taaggaacag aagtctggaa
      121 tataccattt tcaatccagt aaagggtgtt tgcccctggg cgtaaattaa aggcggtgag
      181 atatgcatca gccgcttccc ggttcatccc cttcatttca taaaccttgc caagcaacac
      241 ataatttagc caggacattt caagatcaat gccagtattt atcgcctggt aagactcatc
      301 tgttttacct tttaccagag cactgaccgc ttttatttga tatataatgg acaggttatt
      361 caattccggc agtgtaacaa tgttgtctat ttctgtgttc agtgctgcta attgtttttc
      421 atctaaagga tgttgagaat ggcgcacgat atcaactaat gctttttctg ctctcgcgta
      481 ggtaaattct ggggatgatt gaacaatctc acctaataat tcactggcac gattcaatga
      541 tttatcatcg ccatgcagta aataatcatg tgcctgataa aaattagtta ataacgcacc
      601 acgatgaggc aatatttgct ggagcgtctc ctgcattcgt tgtggccacg gttggtttaa
      661 cgcttttgat aaactctcca gtaaatcatt ttgaatcgcc agctgattac cgttagtgat
      721 gacataacgt ttatccagca tggttgaacc atctgcattg tctaccaatt ttatcgacat
      781 aaagcattgt tgagcacggt attggcgctg attaacaaac gcaatagata atgttttacc
      841 ggaactgctc ggttcatcaa tgttgtagtt gattttgtca tgcaccataa aggtggagaa
      901 ggtgttaagt gatgtcgcca ccaaatcacc cacgcctatc gcgtaagaga gctgatacgg
      961 ggaactccag ctgttacaac ttttatttac catattaatg tcaatatcgc gtggattgag
     1021 caaaatacgc gatttgctca taggaagacg tgtatcaaga gttgaaaacg ctaccagtgc
     1081 tacacagata cctaacgaca acaggaaaaa aaaccatacc caaaaggtag tgaatcgttt
     1141 gcttttaact ggggattgtt caggtggcgt tgcggtgttt tgaatgttaa gactgtggga
     1201 gggagaatct gtggcaggaa ccgcctctgg tataggggga ggcgaagata gcattatttc
     1261 ctctccctct tcttcgctgt accagataac cggcaccatt aatttatagc cgcgctttgg
     1321 tacagtagcg atatagacag gactatcttc atcattatct tttaatgact tacgtagttc
     1381 tgagatactc tgcgtcacaa catgattggt gacaatactt ctcttccaga cattatcgat
     1441 aagttcatcc ctgctaagta cttcgccact gtgttgagca aagaaaacca gaagatcgat
     1501 taatctcggc tcaagggtaa gttgacgccc attgcggcta atttggttta tggacggagt
     1561 aacaagccat tcgccaacgc gaactacagg ttgttgcata atagaaactc attcgaaaag
     1621 ggaatgatgc aatgataatt gccacaacct atttttacca tctatagatg ggtttattta
     1681 catattattg gtgaatgcaa gacgttattt ttaccag
//
LOCUS       DAZKHF020000466         1716 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2201S31241
            SAMN43267800-rid25106413.denovo.469, whole genome shotgun sequence.
ACCESSION   DAZKHF020000466 DAZKHF020000000
VERSION     DAZKHF020000466.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267800
            Sequence Read Archive: SRR30307263
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1716)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1716)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 1716)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350581.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350581.2
            Genome Coverage       :: 151x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:21:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,652
            CDSs (total)                      :: 5,557
            Genes (coding)                    :: 5,320
            CDSs (with protein)               :: 5,320
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 237
            CDSs (without protein)            :: 237
            Pseudo Genes (ambiguous residues) :: 0 of 237
            Pseudo Genes (frameshifted)       :: 102 of 237
            Pseudo Genes (incomplete)         :: 130 of 237
            Pseudo Genes (internal stop)      :: 52 of 237
            Pseudo Genes (multiple problems)  :: 41 of 237
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1716
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267800-rid25106413.denovo.469"
                     /serotype="O 26:H11"
                     /isolate="2201S31241"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-01"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            complement(450..1427)
                     /locus_tag="ACHOSC_RS24760"
                     /old_locus_tag="pgaptmp_004952"
                     /pseudo
     CDS             complement(450..1427)
                     /locus_tag="ACHOSC_RS24760"
                     /old_locus_tag="pgaptmp_004952"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_041206064.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS630 family transposase"
BASE COUNT          424 a          399 c          390 g          503 t
ORIGIN      
        1 aggtgctaat gtgataaaaa aatatttatc tcgatgctac ccaacatacg actcatttat
       61 ttttctcggt ttctccttgt atatatatgt tgctttataa ttttactacg cacgtcaggc
      121 gtatctgtaa catagctccg cattatgcaa tgcgactttg catacaaata ggaataaatc
      181 taatagctgt agatttgctc ctgtatttcc tgaaccaagc gagccagtac atgatgcata
      241 tgtctggaaa ataattcaga gtaaggataa gtccagtcca gctttgttga ataaatcagg
      301 tttgggataa gccccctcag ttggtagcta ctgcaggtaa tacgcacgta ttatgacatt
      361 cgtgccctcg tcattttgtt tagtacacgc actgtagtgg cacagaactt ctgcgacctg
      421 accatcctaa atagctgcgc cgaataccgc tacactttta acagcccata tttaccaccg
      481 gggaatgggc tcacggtttc cataaaataa tgaacctttt taagcagtta ccacattgaa
      541 cggcactgat gattgcgcgt tatcgtatcg tgaagcgcct gctataaccg ctcaacatga
      601 ttcacccacg gcgagtaaac tggctgataa atcactctga acttcggatt ttctttcagc
      661 cagcgctgtg tttcccgact tttatggatg atgtagttgt ccactatgag cgtgataggt
      721 ttatcccgtc ggtatgtcaa tttcagatgt ttcaacagac tgataaataa ggctgagctt
      781 ttgctgttgc cacccacata gctgactttg cccgttccac tatgtaaagc accagccaga
      841 tagtattttt cattctgccc cggtgttacc acatgttttt gctgtctgcg aagttgccag
      901 tccgcaccgg ttttgggatt aagatagata tacacttcat cttcataaaa gaccggatgc
      961 tccgcactac attcgtccag tgctttgtga attgctgctt tatgcgggtt acgggtacgc
     1021 agggttggcg cagccctgcg ccacacaagc ccgactaatg gcaaccagcg gcgaacggtt
     1081 ccggcatgta actggcactc ggttatctcg ttgattttta ttgccagcag ttctgtactc
     1141 cagcgtgaac gctgatagcc aaaatatccg ggagagtgtt ttatcagttc acgaaacaat
     1201 gtgcagatat gctcaaacgg ccagcggcgg gaacgtcctg cgggtaatga tttcagacct
     1261 tcaacactcg acagcgtgaa ccagttaatc cagcgcctga cggaggaacg ggcgcagcag
     1321 agcgttctgg caacgtcact gacgcgagcg ctccggtgca gcatcagcat ggcggtcagc
     1381 ctgcgggcat aatttttatc gtgagtttta tagattgctt tttgcatcag gcgtcattcg
     1441 tcgcggggaa ttggtgctat gatcggcatc gctcagtccg gttagggggg gatttttggc
     1501 gattgatcag atcgcacaat ccgggctgag ttccctttca ttgatctact attcggcgca
     1561 gctatttagt tggtgatttt gaacttctgc tttgctacta agcagcctgc gttttcagga
     1621 aaatacgtga tgtgatcctg caactcagca acagttcggt tgattcaaca aagcgttttt
     1681 taacaataat tcttcattgt aaaaagcatc ttcagt
//
LOCUS       DAZKHF020000467         1712 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2201S31241
            SAMN43267800-rid25106413.denovo.470, whole genome shotgun sequence.
ACCESSION   DAZKHF020000467 DAZKHF020000000
VERSION     DAZKHF020000467.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267800
            Sequence Read Archive: SRR30307263
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1712)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1712)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 1712)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350581.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350581.2
            Genome Coverage       :: 151x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:21:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,652
            CDSs (total)                      :: 5,557
            Genes (coding)                    :: 5,320
            CDSs (with protein)               :: 5,320
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 237
            CDSs (without protein)            :: 237
            Pseudo Genes (ambiguous residues) :: 0 of 237
            Pseudo Genes (frameshifted)       :: 102 of 237
            Pseudo Genes (incomplete)         :: 130 of 237
            Pseudo Genes (internal stop)      :: 52 of 237
            Pseudo Genes (multiple problems)  :: 41 of 237
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1712
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267800-rid25106413.denovo.470"
                     /serotype="O 26:H11"
                     /isolate="2201S31241"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-01"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            complement(<1..404)
                     /locus_tag="ACHOSC_RS24765"
                     /old_locus_tag="pgaptmp_004953"
     CDS             complement(<1..404)
                     /locus_tag="ACHOSC_RS24765"
                     /old_locus_tag="pgaptmp_004953"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdaU family protein"
                     /protein_id="HGU4550706.1"
                     /translation="MLFVLILSHRAASYGAIMAALPYMQLYIADYLADTMHLSAEEHG
                     AYLLLMFNYWQTGKPIPKNRLAKIARLTNERWADVEPSLQEFFCDNGEEWVHLRIEED
                     LASVREKLTKKSAAGKASVQARRSRKEADVQT"
     gene            complement(417..839)
                     /locus_tag="ACHOSC_RS24770"
                     /old_locus_tag="pgaptmp_004954"
     CDS             complement(417..839)
                     /locus_tag="ACHOSC_RS24770"
                     /old_locus_tag="pgaptmp_004954"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxin YdaT family protein"
                     /protein_id="HGU4550707.1"
                     /translation="MKIKHEHIESVLLALAAEKGQAWVANAITEEYLRQGGGELPLVP
                     GKDWNNQQNIYHRWLKGETKTQREKIQKLIPAILAILPRELRHRLCIFDTLERRALLA
                     AQEALSTAIDAHDDAVQAVYRKAHFSGGGSSDDSVIVH"
     gene            complement(836..1090)
                     /locus_tag="ACHOSC_RS24775"
                     /old_locus_tag="pgaptmp_004955"
     CDS             complement(836..1090)
                     /locus_tag="ACHOSC_RS24775"
                     /old_locus_tag="pgaptmp_004955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HGU4550708.1"
                     /translation="MNQKTLEDVIKTVRVSVVADVCGVSQRAIYKWMDNGKLPRTEYT
                     GETNYAEKIAHASNGLFSADVILTIGRNKTTTKKLMGVDS"
     gene            1170..1589
                     /locus_tag="ACHOSC_RS24780"
                     /old_locus_tag="pgaptmp_004956"
     CDS             1170..1589
                     /locus_tag="ACHOSC_RS24780"
                     /old_locus_tag="pgaptmp_004956"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309968.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HGU4550709.1"
                     /translation="MVHEDKARKEFASRLALACENAGYEQHGRQAEIARRMKLTPKAV
                     SKWFNGETIPRREKLRELATLIGTTPTYLLGEDTEESGQVRFYQELNPRQKIIIDLLD
                     ELPDSETDELLKTLEEKKQKYNAIYEELARKKKQKAS"
BASE COUNT          477 a          401 c          354 g          480 t
ORIGIN      
        1 tttgtttgaa catctgcttc ctttctgctt cttctggcct gaacagatgc ttttcctgcg
       61 gctgattttt tggttaattt ttccctgact gatgccagat cttcctcaat ccgaagatgc
      121 acccattcct cgccgttatc gcaaaaaaac tcctgcaagg atggttcaac atcagcccat
      181 cgctcgttag tcagacgggc aatttttgcc agcctgtttt taggtattgg ctttcctgtt
      241 tgccagtaat tgaacatcag caacaaatac gcaccatgct cctctgctga caaatgcatg
      301 gtgtcagcca ggtaatcagc tatgtacagt tgcatgtatg gtaatgcggc cataattgcc
      361 ccgtatgatg ctgcccggtg gcttagaata agcacaaaca gcatggaaac ttttgcttaa
      421 tgaacaatga cagaatcgtc ggaagacccg ccgccgctga aatgcgcttt ccggtaaacg
      481 gcttggactg catcatcatg cgcatcaatt gccgtactta acgcttcctg cgccgccagt
      541 aatgcacggc gttccagggt atcgaagatg cagagtcggt gacgcagctc gcgcggaagg
      601 attgccagaa ttgctgggat cagcttctga attttttctc tttgcgtttt cgtttcacct
      661 ttcaaccaac ggtgatagat attctgctga ttattccagt ccttgcctgg taccaggggc
      721 aattcgccgc ccccctggcg cagatattct tcagtaatgg cattggctac ccatgcctgc
      781 cctttttcgg ctgccagggc taacaacact gactcgatgt gctcatgctt gattttcatg
      841 aatcaactcc catcagcttt ttcgtagtag ttttatttct gccaatagtt aaaattacat
      901 cagcagaaaa taatccgttt gatgcatgag cgattttttc agcgtaattt gtttcgccgg
      961 tatattctgt gcgaggcaat tttccgttat ccatccattt gtagattgct ctttggctga
     1021 caccacaaac gtcggccaca acagaaacgc gaacagtttt gattacatct tcaagtgttt
     1081 tctggttcat atcaccctca caatgtgaac tttgagtaca tgctataaca gaactgacag
     1141 tacattcaag agcgaatatc attgaactta tggttcatga agataaagcg cgtaaagagt
     1201 tcgccagtag gcttgcgcta gcctgtgaaa acgctggtta tgaacaacat ggaaggcagg
     1261 cagaaattgc ccgtcgaatg aaattaacac caaaagcggt tagcaaatgg tttaatggcg
     1321 aaacaattcc tcgccgagag aaattaaggg aattagcaac actcattgga acaacaccaa
     1381 cctatctttt gggagaggat acagaagaaa gtggacaggt acgtttctat caggagttaa
     1441 atccaagaca aaaaatcatc attgaccttc tggacgagct ccctgacagt gagacagatg
     1501 aacttttaaa aactcttgag gagaaaaaac agaagtacaa tgcaatttac gaagagctag
     1561 cacgaaagaa aaaacaaaaa gcctcttaaa ccagcataaa tccggtagcg tccccctccg
     1621 ggtttgtgct tcactttatc ccatctcatt tttttacaca caaaatgtac ttaaagtact
     1681 ttacatcaat gaacacaaag tacattatat ac
//
LOCUS       DAZKHF020000468         1710 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2201S31241
            SAMN43267800-rid25106413.denovo.471, whole genome shotgun sequence.
ACCESSION   DAZKHF020000468 DAZKHF020000000
VERSION     DAZKHF020000468.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267800
            Sequence Read Archive: SRR30307263
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1710)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1710)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 1710)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350581.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350581.2
            Genome Coverage       :: 151x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:21:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,652
            CDSs (total)                      :: 5,557
            Genes (coding)                    :: 5,320
            CDSs (with protein)               :: 5,320
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 237
            CDSs (without protein)            :: 237
            Pseudo Genes (ambiguous residues) :: 0 of 237
            Pseudo Genes (frameshifted)       :: 102 of 237
            Pseudo Genes (incomplete)         :: 130 of 237
            Pseudo Genes (internal stop)      :: 52 of 237
            Pseudo Genes (multiple problems)  :: 41 of 237
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1710
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267800-rid25106413.denovo.471"
                     /serotype="O 26:H11"
                     /isolate="2201S31241"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-01"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            complement(35..355)
                     /locus_tag="ACHOSC_RS24785"
                     /old_locus_tag="pgaptmp_004957"
     CDS             complement(35..355)
                     /locus_tag="ACHOSC_RS24785"
                     /old_locus_tag="pgaptmp_004957"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000818612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Arc family DNA-binding protein"
                     /protein_id="HGU4550710.1"
                     /translation="MAEKQVKDYDKFNLRFPDGMRDAIAERAKRNGRSMNSEIVQILE
                     DALNAENTLGEIADKINSVSVPLNVDALVQLQAQVIAMQKEIQEKFREQNEKLRELLN
                     KKPT"
     gene            463..636
                     /locus_tag="ACHOSC_RS24790"
                     /old_locus_tag="pgaptmp_004958"
     CDS             463..636
                     /locus_tag="ACHOSC_RS24790"
                     /old_locus_tag="pgaptmp_004958"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004177050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Arc family DNA-binding protein"
                     /protein_id="HGU4550711.1"
                     /translation="MQDTLFTERKNIKLNLRLPSRLNEDLRRLAEMDCISLNSAIVRL
                     LAKGVREEVANGR"
     gene            707..1630
                     /locus_tag="ACHOSC_RS24795"
                     /old_locus_tag="pgaptmp_004959"
     CDS             707..1630
                     /locus_tag="ACHOSC_RS24795"
                     /old_locus_tag="pgaptmp_004959"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001428824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage antirepressor N-terminal domain-containing
                     protein"
                     /protein_id="HGU4550712.1"
                     /translation="MTSLAIADRTINVPFHGTNLFLVGINNEPYVPMKPVVEGMGMVW
                     AAQFVKLKQRFVKGISEIEIPSAGGKQLMTCLAFRKFAAWLSSIQPNKVRPEIRDKVI
                     QYQEECDDVLYEYWTKGHVINPRKAKKALPGKITTEQQEAIKQLVMSRGQSLPKEKQA
                     KAMITMWSSLKSHFGCSYKEISEEQFTEALSLAARVPLEGELIGKQEKKTNELSAKEA
                     NSLVWLWDYANRSQALFRELYPALKQIQSNYSCRCYDYGHEFSYVIGMARDVLINHTR
                     DIDINEPDGPTNLSAWLRLKNKELPPSLHHY"
BASE COUNT          486 a          371 c          374 g          479 t
ORIGIN      
        1 aataaacttt cgcagtaatg gattatgccc accgtcaggt gggtttttta tttagtagtt
       61 ctctcaactt ttcgttctgc tctctgaact tttcctgtat ttctttttgc atggcgataa
      121 cctgggcttg aagttgaact agcgcatcaa catttagcgg aaccgagacg ctgttaattt
      181 tatctgctat ttccccgagt gtattttctg cattcaaggc atcttccagt atctgaacaa
      241 tctctgagtt catagagcgc ccgtttcgtt tggctcgttc agctatagca tctcgcattc
      301 cgtcaggaaa acggaggttg aacttgtcgt agtcttttac ttgtttttcg gccattgcaa
      361 atctctcaaa aaaaatcatg gtgccatatt gccatacgat ttcaatagtg gcattatggc
      421 cttcatggtg tcactttggc accaaataaa ggagattaga taatgcaaga tacacttttc
      481 actgagcgca aaaatatcaa actcaacctt cgccttccat cacggctgaa tgaagacctt
      541 cgccgcctgg cggaaatgga ctgtatatct ctgaactctg caattgttcg tttgctggca
      601 aaaggtgtta gggaagaggt ggcgaatggt cgctaaaaac agcgaagcct caatggctgc
      661 aaccattgag gcttctaaat taccagttaa ccacgagaaa actgatatga ctagtttagc
      721 aattgcagat cgcacaatca atgttccatt ccacggaaca aatctctttt tggttggaat
      781 taacaatgag ccttatgttc ctatgaagcc tgttgttgaa ggtatgggga tggtttgggc
      841 tgctcaattt gttaagttaa aacagaggtt tgtcaaaggt atttcggaaa tcgaaatacc
      901 ttctgctggc ggtaaacagt taatgacatg tcttgccttt cgtaaatttg cggcttggct
      961 ttcaagcatt caaccaaaca aagtccgccc tgaaatccgc gacaaggtaa tccagtatca
     1021 ggaagagtgt gacgatgtgc tctacgagta ctggactaaa ggccatgtga ttaacccgcg
     1081 caaagctaaa aaggcattgc cggggaaaat caccactgaa cagcaggaag ccattaaaca
     1141 actcgtcatg agtcgcggtc agtctctgcc aaaagaaaaa caggcgaagg cgatgatcac
     1201 catgtggtcg tcactgaaat ctcattttgg gtgttcatac aaagaaatca gcgaggagca
     1261 gtttaccgaa gctctgtcac ttgctgctcg cgttccgctt gaaggagagt taatcggcaa
     1321 acaagagaag aaaaccaacg agctttctgc aaaagaagca aacagccttg tatggttatg
     1381 ggattatgct aaccgctcac aggcattatt ccgcgaactg tatccggcgc taaagcaaat
     1441 tcaatcgaac tattcctgca gatgctacga ctacggtcat gagttctcgt atgttattgg
     1501 aatggcgaga gacgttttaa tcaatcacac gcgagatatt gatattaatg agccagacgg
     1561 accaacgaat ctttctgcat ggctaagact taagaacaaa gaattacctc cttcactgca
     1621 tcactactaa cagattgcca acgaaatgac ccagcttcgg ctgggttttt tatcaggagt
     1681 tctcatgctc tatagcaata tattggcgca
//
LOCUS       DAZKHF020000469         1701 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2201S31241
            SAMN43267800-rid25106413.denovo.472, whole genome shotgun sequence.
ACCESSION   DAZKHF020000469 DAZKHF020000000
VERSION     DAZKHF020000469.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267800
            Sequence Read Archive: SRR30307263
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1701)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1701)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 1701)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350581.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350581.2
            Genome Coverage       :: 151x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:21:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,652
            CDSs (total)                      :: 5,557
            Genes (coding)                    :: 5,320
            CDSs (with protein)               :: 5,320
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 237
            CDSs (without protein)            :: 237
            Pseudo Genes (ambiguous residues) :: 0 of 237
            Pseudo Genes (frameshifted)       :: 102 of 237
            Pseudo Genes (incomplete)         :: 130 of 237
            Pseudo Genes (internal stop)      :: 52 of 237
            Pseudo Genes (multiple problems)  :: 41 of 237
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1701
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267800-rid25106413.denovo.472"
                     /serotype="O 26:H11"
                     /isolate="2201S31241"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-01"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            complement(<1..67)
                     /locus_tag="ACHOSC_RS24800"
                     /old_locus_tag="pgaptmp_004960"
     CDS             complement(<1..67)
                     /locus_tag="ACHOSC_RS24800"
                     /old_locus_tag="pgaptmp_004960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HGU4550713.1"
                     /translation="MKHTDIRAAVLDALEQHEHGAT"
     gene            complement(64..639)
                     /locus_tag="ACHOSC_RS24805"
                     /old_locus_tag="pgaptmp_004961"
     CDS             complement(64..639)
                     /locus_tag="ACHOSC_RS24805"
                     /old_locus_tag="pgaptmp_004961"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HGU4550714.1"
                     /translation="MAIKGLDQAIENLSRVRKNAIPSASAMAINRVATTAINQSSSQV
                     ARETRVSRKLVKERSRLKRATVRNPNARIIVNRGDLPAIKLGIRMLGHRPNSILKAGQ
                     HRYQRAFIQRLNNGRWHVMQRLPEARYAKGNDDKGRKKRNRLPIQVVKIPMAAPLKQA
                     FDENVNRIRRERLPKELGYALKQQLRIVIKR"
     gene            complement(654..1007)
                     /locus_tag="ACHOSC_RS24810"
                     /old_locus_tag="pgaptmp_004962"
     CDS             complement(654..1007)
                     /locus_tag="ACHOSC_RS24810"
                     /old_locus_tag="pgaptmp_004962"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001204533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="head-tail joining protein"
                     /protein_id="HGU4550715.1"
                     /translation="MRDFQNAFDAALAGVDSTIVEVMGISAQFTSGAQRGGEVQGVFD
                     DPESLGFASSGIRIEGSSPSLFVRTDTVRAVRRGDTLTINGETFWVDRVSPDDGGSCY
                     LWLNRGQPPAVNRRR"
     gene            complement(1000..1374)
                     /gene="gpFI"
                     /locus_tag="ACHOSC_RS24815"
                     /old_locus_tag="pgaptmp_004963"
     CDS             complement(1000..1374)
                     /gene="gpFI"
                     /locus_tag="ACHOSC_RS24815"
                     /old_locus_tag="pgaptmp_004963"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000201530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-packaging protein FI"
                     /protein_id="HGU4550716.1"
                     /translation="MATKEQNLKRLDELALILGREPDISGSAAEIAQRVAEWEEEMQS
                     SGDDVQVMNMDIRERENAAHDVREETSGALTRIRVLTCLHLCGVDGETGESVELADVG
                     RVILIMSSDAKTHVDGGMAVYA"
     gene            complement(1426..>1701)
                     /locus_tag="ACHOSC_RS24820"
                     /old_locus_tag="pgaptmp_004964"
     CDS             complement(1426..>1701)
                     /locus_tag="ACHOSC_RS24820"
                     /old_locus_tag="pgaptmp_004964"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="major capsid protein"
                     /protein_id="HGU4550717.1"
                     /translation="VWRLLNGFKLFREKLDTRRGSNSQLETAVKDLGAVVSFKGYYGD
                     LAIVVASSRYPKHWLTVGDPAREFTMTQSAPLMVLPDPDEFVVVQVK"
BASE COUNT          390 a          531 c          375 g          405 t
ORIGIN      
        1 gcgtcgcccc gtgttcatgc tgctcgagtg catccagcac tgcagcacgg atatcagtat
       61 gtttcatcgc tttatcacaa tccttagttg ttgtttcaac gcatagccca gttcttttgg
      121 cagacgttct cgccggatac ggttaacgtt ctcatcaaaa gcctgcttca gtggggccgc
      181 catcggaatt ttaaccacct gaatgggaag acgattacgc tttttccttc ctttatcgtc
      241 attgcccttc gcataccggg cttctggcaa acgttgcata acatgccagc gcccattatt
      301 taatcgctgg atgaatgccc gctgataacg atgctgaccg gctttaagta tgctgttcgg
      361 acgatgacca agcatcctga tccccagctt aatagcaggg agatcaccgc ggttaacgat
      421 aattctggca ttcggatttc tgaccgtcgc ccgtttcagt ctggaccgtt cctttaccag
      481 tttccggctc accctggtct cccgggcaac ctgtgatgaa gactgattaa tcgccgttgt
      541 agccacgcgg ttaatagcca ttgctgaagc cgacggaatg gcgtttttac gaacccggct
      601 cagattttca atcgcctgat caagcccttt tatcgccata attcaccctg cgtttatcgt
      661 cgccggttaa ctgcgggtgg ttgcccacgg ttcagccaga gataacaact gcccccgtca
      721 tccggagaaa cacgatccac ccagaacgtc tcaccattaa tggtcaacgt gtcaccacgc
      781 cgcacggcac gaaccgtatc cgtccgcaca aataatgacg ggctgcttcc ttcaatacgg
      841 atcccactac tggcaaaccc cagcgactcc ggatcgtcaa aaaccccctg aacttcgccg
      901 ccacgctgtg caccggaggt gaactgcgca ctgatgccca tcacttcaac aatcgtactg
      961 tccaccccgg caagggcggc atcaaaggca ttctgaaaat cacgcataaa cagccattcc
     1021 accatcaacg tgtgtttttg catctgagga cataatcaga atcacccgac caacatccgc
     1081 aagctcaacg gattcccccg tttcaccatc aacgccacag agatggaggc aggtcagaac
     1141 tctgatgcgc gttaacgcgc cggatgtttc ctcacgaaca tcatgagccg cgttttccct
     1201 ctcccggata tccatattca taacctgtac atcatcgccg gatgactgca tttcctcttc
     1261 ccattctgcc acccgctgcg ctatctctgc ggcactcccg gatatatccg gctcacgccc
     1321 cagaatcagg gccagttcat caagccgttt cagattttgc tctttcgttg ccatatcatc
     1381 cccctgtgaa aaaagacacg ggggcatttc gcccccgctc acggattatt tcacctgtac
     1441 caccacaaac tcatccgggt ccggcaacac catcagcggc gcggactgcg tcatggtaaa
     1501 ttcacgggcg ggatccccta ccgtcagcca gtgtttcgga taacgggaag aggccaccac
     1561 aatggccaga tcgccgtaat accctttgaa ggacaccacc gcgcccaggt ctttcaccgc
     1621 tgtttcgagt tgcgaattag agccgcgacg ggtatccagt ttttcgcgga acaatttaaa
     1681 gccattcagc agacgccaga c
//
LOCUS       DAZKHF020000470         1697 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2201S31241
            SAMN43267800-rid25106413.denovo.473, whole genome shotgun sequence.
ACCESSION   DAZKHF020000470 DAZKHF020000000
VERSION     DAZKHF020000470.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267800
            Sequence Read Archive: SRR30307263
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1697)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1697)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 1697)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350581.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350581.2
            Genome Coverage       :: 151x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:21:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,652
            CDSs (total)                      :: 5,557
            Genes (coding)                    :: 5,320
            CDSs (with protein)               :: 5,320
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 237
            CDSs (without protein)            :: 237
            Pseudo Genes (ambiguous residues) :: 0 of 237
            Pseudo Genes (frameshifted)       :: 102 of 237
            Pseudo Genes (incomplete)         :: 130 of 237
            Pseudo Genes (internal stop)      :: 52 of 237
            Pseudo Genes (multiple problems)  :: 41 of 237
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1697
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267800-rid25106413.denovo.473"
                     /serotype="O 26:H11"
                     /isolate="2201S31241"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-01"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            complement(895..1035)
                     /locus_tag="ACHOSC_RS24825"
                     /old_locus_tag="pgaptmp_004965"
     CDS             complement(895..1035)
                     /locus_tag="ACHOSC_RS24825"
                     /old_locus_tag="pgaptmp_004965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001135715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hok/Gef family protein"
                     /protein_id="HGU4550718.1"
                     /translation="MPQKTIIVGMLCLTMLLTVWVLHASPCEFRVSFMWSEIAAFLQC
                     KP"
     gene            complement(1337..1600)
                     /locus_tag="ACHOSC_RS24830"
                     /old_locus_tag="pgaptmp_004966"
     CDS             complement(1337..1600)
                     /locus_tag="ACHOSC_RS24830"
                     /old_locus_tag="pgaptmp_004966"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type B 50S ribosomal protein L31"
                     /protein_id="HGU4550719.1"
                     /translation="MKPNIHPEYRTVVFHDTSIDEYFRIGSTIKTDRVIELDGVTYPY
                     VTIDVSSKSHPFYTGKLRTVASEGNVARFTQRFGRFVDAKKGG"
BASE COUNT          494 a          405 c          333 g          465 t
ORIGIN      
        1 aacaacgaaa atgttcagga ataaaaaaaa gagacaattc ccgagataaa acaaaagagc
       61 ataaacatat gaaccggaaa ttctatttca aaaatatcct gtggggaatg tgccccatta
      121 taatatccat attgcatctg atatatgaca caccaacaga agcatcgata aaagttgact
      181 tatttctgat actcagtggc ctggcccttc cttgttcaat atacctgaca aaaacaatca
      241 cactaaaata cacatccata tcattctgga aaaaacctct tttctggtct tatggtatat
      301 atcattttca tcttgtttat ataatgacat gtatagtttt agcaatacct tctgcaatta
      361 tcgttttatt ctttcacttt tatcaaaaca tcaccagaaa ctggaagtga caaccgcaat
      421 aatgaacata aaaaggcaaa tcagaatggc agtaaatcaa taaagtacca tttcatatga
      481 atcatcagaa aaacggtgga cgcgacatcc agcacgaggc aaaacacagc aacaaaataa
      541 gccggatatc gcgccctgta cttttctaac tcaactcccg gcattgtgca agttttaatc
      601 gcaggtggat tcagagccac gcccccattg attcagcctg aagacacgta tatccttgga
      661 gaaagcagga atctcattgc tgtgaaattc cttacagaat cttcagcccc attttatggg
      721 gctttttttg gtgcccccac gctgttacca cactttcggg atgcgaataa aaaagcccac
      781 agtttttaca ctgcggactt ttgttttttc tttgtgattt gtgtgcaagg gtgcgacttt
      841 cgaccgaacc tttggcaacc ggatagcggg gactgttgtc cccgctgcgg tttcttacgg
      901 tttacactgc aagaacgccg caatttccga ccacatgaag cttaccctga attcacacgg
      961 tgatgcgtgt aacacccaga cggtcagcaa catggtcagg cataacatgc ccacaatgat
     1021 cgttttttgc ggcatagcac ttgctccttt tacagagagg cgctaacctt ccacttgctg
     1081 aaggtagatg gtcagggcct cgggttaacg tttaagttga ctcggggcct ttccacatcc
     1141 ggccttcagg taatccttcc agccgtcagc cgaaaggcac ccgcgcgtaa tgtactggtt
     1201 ttcagagagg tccgcaatct tccggtgacg agatcatctg cacacgcccc ggtgggacgg
     1261 gctaacggta tattcaggtg caggatctct ctgtctggtg cgtttttaaa ttgtcaaacg
     1321 caccctggcg gcaaaatcag ccaccttttt tcgcatcaac aaaacgccca aaacgctggg
     1381 taaagcgtgc aacattacct tctgaagcga cagttctcag tttacctgta taaaacggat
     1441 gcgacttaga agaaacgtca attgtaacat atgggtaagt tacaccatcc agttcaatta
     1501 cccggtcagt tttgatcgtc gaaccgatcc tgaagtactc atcaatactg gtatcgtgaa
     1561 atactactgt acggtattcc ggatggatat tcggtttcac aacagttttc cccctttaag
     1621 tgaaaattcc gcataaaggt acatattcag acggtcagat tcaactgtct gttcttataa
     1681 cctcactctc tttcagt
//
LOCUS       DAZKHF020000471         1688 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2201S31241
            SAMN43267800-rid25106413.denovo.474, whole genome shotgun sequence.
ACCESSION   DAZKHF020000471 DAZKHF020000000
VERSION     DAZKHF020000471.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267800
            Sequence Read Archive: SRR30307263
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1688)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1688)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 1688)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350581.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350581.2
            Genome Coverage       :: 151x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:21:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,652
            CDSs (total)                      :: 5,557
            Genes (coding)                    :: 5,320
            CDSs (with protein)               :: 5,320
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 237
            CDSs (without protein)            :: 237
            Pseudo Genes (ambiguous residues) :: 0 of 237
            Pseudo Genes (frameshifted)       :: 102 of 237
            Pseudo Genes (incomplete)         :: 130 of 237
            Pseudo Genes (internal stop)      :: 52 of 237
            Pseudo Genes (multiple problems)  :: 41 of 237
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1688
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267800-rid25106413.denovo.474"
                     /serotype="O 26:H11"
                     /isolate="2201S31241"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-01"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            52..159
                     /locus_tag="ACHOSC_RS24835"
                     /old_locus_tag="pgaptmp_004967"
     CDS             52..159
                     /locus_tag="ACHOSC_RS24835"
                     /old_locus_tag="pgaptmp_004967"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4550720.1"
                     /translation="MRKSYEVGISPKINLCNSVEVLTNSFGTVISGRQV"
     gene            365..1648
                     /gene="gltA"
                     /locus_tag="ACHOSC_RS24840"
                     /old_locus_tag="pgaptmp_004968"
     CDS             365..1648
                     /gene="gltA"
                     /locus_tag="ACHOSC_RS24840"
                     /old_locus_tag="pgaptmp_004968"
                     /EC_number="2.3.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004108 - citrate (Si)-synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="citrate synthase"
                     /protein_id="HGU4550721.1"
                     /translation="MADTKAKLTLNGDTAVELDVLKGTLGQDVIDIRTLGSKGVFTFD
                     PGFTSTASCESKITFIDGDEGILLHRGFPIDQLATDSNYLEVCYILLNGEKPTQEQYD
                     EFKTTVTRHTMIHEQITRLFHAFRRDSHPMAVMCGITGALAAFYHDSLDVNNPRHREI
                     AAFRLLSKMPTMAAMCYKYSIGQPFVYPRNDLSYAGNFLNMMFSTPCEPYEVNPILER
                     AMDRILILHADHEQNASTSTVRTAGSSGANPFACIAAGIASLWGPAHGGANEAALKML
                     EEISSVKHIPEFVRRAKDKNDSFRLMGFGHRVYKNYDPRATVMRETCHEVLKELGTKD
                     DLLEVAMELENIALNDPYFIEKKLYPNVDFYSGIILKAMGIPSSMFTVIFAMARTVGW
                     IAHWSEMHSDGMKIARPRQLYTGYEKRDFKSDIKR"
BASE COUNT          439 a          410 c          396 g          443 t
ORIGIN      
        1 ttgacaatca ttcaacaaag ttgttacaaa cattaccagg aaaagcatat aatgcgtaaa
       61 agttatgaag tcggtatttc acctaagatt aacttatgta acagtgtgga agtattgacc
      121 aattcattcg ggacagttat tagtggtaga caagtttaat aattcggatt gctaagtact
      181 tgattcgcca tttattcgtc atcaatggat cctttacctg caagcgccca gagctctgta
      241 cccaggtttt cccctctttc acagagcggc gagccaaata acaaacgggt aaagccaggt
      301 tgatgtgcga aggcaaattt aagttccggc agtcttacgt aataaggcgc taaggagacc
      361 ttaaatggct gatacaaaag caaaactcac cctcaacggg gacacagctg ttgaactgga
      421 tgtgctgaaa ggcacgctgg gtcaagatgt tattgatatc cgtactctcg gttcaaaagg
      481 tgtgttcacc tttgacccag gcttcacttc aaccgcatcc tgcgaatcta aaattacttt
      541 tattgatggt gatgaaggta ttttgctgca ccgcggtttc ccgatcgatc agctggcgac
      601 cgattctaac tacctggaag tttgttacat cctgctgaat ggtgaaaaac cgactcagga
      661 acagtatgac gaatttaaaa ctacggtgac ccgtcatacc atgatccacg agcagattac
      721 ccgtctgttc cacgctttcc gtcgcgactc acatccaatg gcagtcatgt gtggtattac
      781 cggcgcgctg gcggcgttct atcacgactc gctggatgtt aacaatcctc gtcatcgtga
      841 aattgccgcg ttccgcctgc tgtcgaaaat gccgaccatg gccgcgatgt gttacaagta
      901 ttccattggt cagccatttg tttatccgcg caacgatctc tcctatgccg gtaacttcct
      961 gaatatgatg ttctccacgc cgtgcgaacc gtatgaagtt aatccgattc tggaacgtgc
     1021 tatggaccgt attctgatcc tgcacgctga ccatgaacag aacgcctcta cctccaccgt
     1081 gcgtaccgct ggctcttcgg gtgcgaaccc gtttgcctgt atcgcagcag gtattgcttc
     1141 actgtgggga cctgcgcacg gtggtgctaa cgaagcggcg ctgaaaatgc tggaagaaat
     1201 tagctccgtt aaacacattc cggaatttgt tcgtcgtgcg aaagataaaa atgattcttt
     1261 ccgcctgatg ggcttcggtc accgcgtgta caaaaattac gacccgcgcg ccaccgtaat
     1321 gcgtgaaacc tgccatgaag ttctgaaaga gctgggcacc aaagatgacc tgctggaagt
     1381 ggctatggag ctggaaaaca tcgcgctgaa cgacccgtac tttatcgaga agaaactgta
     1441 cccgaacgtc gatttctact ctggtatcat cctgaaagcg atgggtattc cgtcttccat
     1501 gttcaccgtc attttcgcaa tggcacgtac cgttggctgg atcgcccact ggagcgaaat
     1561 gcacagtgac ggtatgaaga ttgcccgtcc gcgtcagctg tatacaggat atgaaaaacg
     1621 cgactttaaa agcgatatca agcgttaatg gttgattgct aagttgtaaa tattttaacc
     1681 cgccgttc
//
LOCUS       DAZKHF020000472         1679 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2201S31241
            SAMN43267800-rid25106413.denovo.475, whole genome shotgun sequence.
ACCESSION   DAZKHF020000472 DAZKHF020000000
VERSION     DAZKHF020000472.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267800
            Sequence Read Archive: SRR30307263
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1679)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1679)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 1679)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350581.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350581.2
            Genome Coverage       :: 151x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:21:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,652
            CDSs (total)                      :: 5,557
            Genes (coding)                    :: 5,320
            CDSs (with protein)               :: 5,320
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 237
            CDSs (without protein)            :: 237
            Pseudo Genes (ambiguous residues) :: 0 of 237
            Pseudo Genes (frameshifted)       :: 102 of 237
            Pseudo Genes (incomplete)         :: 130 of 237
            Pseudo Genes (internal stop)      :: 52 of 237
            Pseudo Genes (multiple problems)  :: 41 of 237
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1679
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267800-rid25106413.denovo.475"
                     /serotype="O 26:H11"
                     /isolate="2201S31241"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-01"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            complement(<1..302)
                     /locus_tag="ACHOSC_RS24845"
                     /old_locus_tag="pgaptmp_004969"
     CDS             complement(<1..302)
                     /locus_tag="ACHOSC_RS24845"
                     /old_locus_tag="pgaptmp_004969"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000426255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4550722.1"
                     /translation="MEMVVSDGEERFTELDFYYGSKALEGTSEIVTVLTNTILNREVV
                     TKIPSIEGITASFRTSYEGSFRQRFMLKITGEEQRRNYGAIGEGAFLDVMSYYLTRP"
     gene            1101..1574
                     /locus_tag="ACHOSC_RS24850"
                     /old_locus_tag="pgaptmp_004970"
     CDS             1101..1574
                     /locus_tag="ACHOSC_RS24850"
                     /old_locus_tag="pgaptmp_004970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000350517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4550723.1"
                     /translation="MDIDTLGGQELRDKIFAGLKIYEGKPFIERFGLFMGKAQLLEFG
                     LKKILVSFPGYNLDEEKLERLTLGQTRVELKKLGLRTDYNAYLKSFKDQRNTMAHEFL
                     ANFAVTQQLLDGAALIRTFERELDHACYAVEQLIILFDFINGAGDVTAWLEPTAP"
BASE COUNT          450 a          381 c          354 g          494 t
ORIGIN      
        1 ggtcttgtaa gataataaga cataacatct agaaatgccc cctctccgat tgcgccatag
       61 tttctacgtt gttcctcacc tgtgatcttg agcatgaaac gttgtctaaa cgacccttca
      121 taactggtac gaaaagatgc ggttatgcct tcgattgatg ggattttagt aactacttcg
      181 cgatttagaa tcgtattagt gaggactgta actatctctg acgtgccttc aagtgctttt
      241 gacccatagt aaaaatcaag ctctgtgaat ctttcttctc catcacttac taccatttcc
      301 atgggaacat gaatttccat tctctaattc caaataaaaa ccatgggttt aatatgtcct
      361 aattgatttg gttttggcaa ctatttttga ttgtttttca agaataattc gaaagctatt
      421 aattaatggc tggaactaat aggttccagt tttcaactaa tccagtgacc tgctccccgc
      481 tgatcaactc agactgttgt tagcaactta cgctcctggt acggagcggg cattgattac
      541 ccgctccgtt cacgttccgc ctggaagttc tgcaatttat gtctatcggc tattgaaaac
      601 cttccggtcc ttcccaatat cgaaactggc acctttgcca gataacaaag agatacggga
      661 acatgatgta cgtctacaga agacctccaa atcagctcct ttccatttct cgtttgaagc
      721 agtatttgtt gcatgaggtg gaatcaaatc aacacatcat gagacagtgg gaatctgcca
      781 gaacggcact tcgaaacatc cgtaacaaag ggcatgatcc agacaccgcc tcctgggttt
      841 cacttaaaaa tgatccccga gtcttcacga atggcgcaga tgaaaagcat gggttcattt
      901 ttgttgacga cgacgatcaa tcagtccttg ttgtggcctc ctgggtgata gatgagtacg
      961 gttcccccct cactacatat taatccctgt acctggacag aagagttttc tgtccacaac
     1021 taccaatagc tttacataat gtgtgcatct aacttcaata agttacttca ttaccaataa
     1081 tgtctaaaaa ggaaacgcgc atggatattg atacgctggg tggccaggaa cttcgggata
     1141 agatttttgc tgggctcaaa atatacgagg gtaaaccctt catcgaacgg ttcggtctgt
     1201 ttatgggcaa ggcacagttg ctagagtttg gcctaaaaaa aattcttgtt tcttttccgg
     1261 gatacaacct tgatgaagaa aaattagaga ggctgacatt aggccagacc cgcgttgagc
     1321 ttaaaaagct ggggctgcgc acggactata atgcttacct gaagtcattc aaagatcagc
     1381 gcaatacgat ggctcacgag ttccttgcta attttgcggt cacacagcag cttttagacg
     1441 gagctgcact tattcgcaca tttgagcgtg agttagacca tgcctgttac gcggtggagc
     1501 agttgatcat ccttttcgac ttcatcaacg gtgccggtga cgtaaccgcc tggcttgagc
     1561 ctactgcccc ctgatacttc gtatgctttt atctctaggc tggacagaat agtaattctg
     1621 tccaggtatg cagaatggct gtctatatat cttcattagt tcacagtagg ttaccggcg
//
LOCUS       DAZKHF020000473         1666 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2201S31241
            SAMN43267800-rid25106413.denovo.476, whole genome shotgun sequence.
ACCESSION   DAZKHF020000473 DAZKHF020000000
VERSION     DAZKHF020000473.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267800
            Sequence Read Archive: SRR30307263
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1666)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1666)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 1666)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350581.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350581.2
            Genome Coverage       :: 151x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:21:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,652
            CDSs (total)                      :: 5,557
            Genes (coding)                    :: 5,320
            CDSs (with protein)               :: 5,320
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 237
            CDSs (without protein)            :: 237
            Pseudo Genes (ambiguous residues) :: 0 of 237
            Pseudo Genes (frameshifted)       :: 102 of 237
            Pseudo Genes (incomplete)         :: 130 of 237
            Pseudo Genes (internal stop)      :: 52 of 237
            Pseudo Genes (multiple problems)  :: 41 of 237
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1666
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267800-rid25106413.denovo.476"
                     /serotype="O 26:H11"
                     /isolate="2201S31241"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-01"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            complement(56..238)
                     /locus_tag="ACHOSC_RS24855"
                     /old_locus_tag="pgaptmp_004971"
     CDS             complement(56..238)
                     /locus_tag="ACHOSC_RS24855"
                     /old_locus_tag="pgaptmp_004971"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4550724.1"
                     /translation="MTLMNEREQNRLIRGLIRQRDAWKTQETGHKDKASGRAERITAK
                     RLTDRDREVMECFRNR"
     gene            complement(222..662)
                     /locus_tag="ACHOSC_RS24860"
                     /old_locus_tag="pgaptmp_004972"
     CDS             complement(222..662)
                     /locus_tag="ACHOSC_RS24860"
                     /old_locus_tag="pgaptmp_004972"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease signature motif containing
                     protein"
                     /protein_id="HGU4550725.1"
                     /translation="MPWQPLRRCTEPGCNRRVKSGKCEEHRRAAWRAEDARRGHRRAR
                     GYSRQWDKYRAMYLSKNPLCVRCLEKGIYTPAVVVDHIIPIDGGDDVLFWPEWNHQPL
                     CQACHNQKTKWLDPATKSKRAAGGFREDEERAANRNNWMYDADE"
     gene            complement(662..955)
                     /locus_tag="ACHOSC_RS24865"
                     /old_locus_tag="pgaptmp_004973"
     CDS             complement(662..955)
                     /locus_tag="ACHOSC_RS24865"
                     /old_locus_tag="pgaptmp_004973"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0016032 - viral process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="head-tail connector protein"
                     /protein_id="HGU4550726.1"
                     /translation="MTELITLEEVKLHCRIDGDDEDQLISGYIAASLEACQIHIGRRF
                     DDGLEFTPAIKIGCMMFIAHLYENRQIVADNAKTRVPMTIGALWTAYRDVGVY"
     gene            complement(952..1290)
                     /locus_tag="ACHOSC_RS24870"
                     /old_locus_tag="pgaptmp_004974"
     CDS             complement(952..1290)
                     /locus_tag="ACHOSC_RS24870"
                     /old_locus_tag="pgaptmp_004974"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage head closure protein"
                     /protein_id="HGU4550727.1"
                     /translation="MNIGRLRDRVTIQTLKQTRAMTGEILETWLDGHTLWASVNMISS
                     KEAISSGAELATGTVRIWIRYRKDINATSRIKVNTGPLAGRVLNIIGQPLPDAARTRL
                     EILCREGAEK"
     gene            complement(1287..>1666)
                     /locus_tag="ACHOSC_RS24875"
                     /old_locus_tag="pgaptmp_004975"
     CDS             complement(1287..>1666)
                     /locus_tag="ACHOSC_RS24875"
                     /old_locus_tag="pgaptmp_004975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309619.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HGU4550728.1"
                     /translation="MFNVSPIFLQEYSNSTYSNFSEASRAFLTMTMRPWLANFEQQIK
                     NALLVASPVPGIRYQVEFDSADLLRATPGERFATYERGIKSGVMCPNEAREREGLSPR
                     DGGDEFSQAWKQEVKISEGEKPE"
BASE COUNT          309 a          503 c          408 g          446 t
ORIGIN      
        1 cccggcgggg tcgggtttgt cctgaaaacg ccccccaccc cgccatacag cctcatcagc
       61 gattgcggaa acattccata acctcgcggt cacggtcggt taatcgcttc gctgtgatgc
      121 gttctgcgcg tcctgacgct ttatctttat gccctgtttc ctgtgtcttc catgcgtcac
      181 gctgccttat aagtccacgg ataaggcggt tttgttcccg ctcattcatc agcgtcatac
      241 atccagttat tgcggttagc ggcccgttct tcatcttcac gaaatccacc tgcggcacgc
      301 ttgctttttg ttgccggatc aagccatttc gttttctggt tatgacacgc ctgacacaat
      361 ggctgatggt tccattcggg ccagaagaga acatcatcgc cgccatcgat ggggataatg
      421 tgatccacca ccacggcggg cgtatatatc cccttctcaa ggcatcgcac gcataacggg
      481 tttttactca gatacatggc gcggtatttg tcccactgtc tggagtaccc acgcgcgcgg
      541 cggtgtcctc gtctggcatc ctctgcacgc catgccgccc gcctgtgctc ctcacactta
      601 ccggacttaa cgcgcctgtt acagcctggt tctgtacatc gtcttaatgg ttgccacggc
      661 atcagtacac ccccacatca cggtaagccg tccagagtgc gcctatcgtc atgggtacgc
      721 gcgtttttgc gttatccgcg acaatctggc gattttcata caggtgagcg ataaacatca
      781 tgcagccaat ctttatggct ggcgtgaact ccagcccgtc atcaaagcgc ctgcctatgt
      841 gtatctggca cgcctcaagc gatgcggcaa tgtatccgct gattaactgg tcttcatcgt
      901 cgccatcgat gcggcaatgg agtttcactt cttccagggt gataagttct gtcatttttc
      961 cgcgccctca cgacaaagaa tttcaaggcg tgtcctggcg gcatctggca gcggttgccc
     1021 gatgatattc agtacacgcc ccgccagcgg ccccgtattg acctttatcc gactggtggc
     1081 gttgatgtcc ttccggtagc gtatccatat ccttacagtt ccagtcgcca gctctgcacc
     1141 tgatgaaatg gcctccttgc tgctgatcat gttcacgctt gcccagagtg tgtgaccgtc
     1201 cagccacgtt tcgagtattt cacccgtcat ggctctggtc tgtttcaggg tctgaatcgt
     1261 taccctgtca cgcagtcgcc ctatgttcat tccggttttt ctccctcgct gatttttact
     1321 tcctgtttcc atgcctggct gaactcatca ccaccatcac gcggtgacag tccttcacgt
     1381 tcgcgggctt cgttcggaca cataacgccg gatttgatgc cgcgctcata ggtggcaaag
     1441 cgttcgccag gtgtggcccg taacaggtcc gcgctgtcaa actccacctg ataccggata
     1501 ccaggtacag gcgaggccac cagcagggcg ttttttatct gctgctcaaa gttcgccagc
     1561 cacgggcgca tcgtcatggt gagaaatgcg cggcttgcct cgctgaaatt gctgtaggtg
     1621 ctgttgctgt attcctgcaa aaaaatcggc gagacgttga acattc
//
LOCUS       DAZKHF020000474         1656 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2201S31241
            SAMN43267800-rid25106413.denovo.477, whole genome shotgun sequence.
ACCESSION   DAZKHF020000474 DAZKHF020000000
VERSION     DAZKHF020000474.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267800
            Sequence Read Archive: SRR30307263
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1656)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1656)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 1656)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350581.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350581.2
            Genome Coverage       :: 151x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:21:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,652
            CDSs (total)                      :: 5,557
            Genes (coding)                    :: 5,320
            CDSs (with protein)               :: 5,320
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 237
            CDSs (without protein)            :: 237
            Pseudo Genes (ambiguous residues) :: 0 of 237
            Pseudo Genes (frameshifted)       :: 102 of 237
            Pseudo Genes (incomplete)         :: 130 of 237
            Pseudo Genes (internal stop)      :: 52 of 237
            Pseudo Genes (multiple problems)  :: 41 of 237
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1656
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267800-rid25106413.denovo.477"
                     /serotype="O 26:H11"
                     /isolate="2201S31241"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-01"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            29..1645
                     /gene="tir"
                     /locus_tag="ACHOSC_RS24880"
                     /old_locus_tag="pgaptmp_004976"
     CDS             29..1645
                     /gene="tir"
                     /locus_tag="ACHOSC_RS24880"
                     /old_locus_tag="pgaptmp_004976"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001121326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system LEE translocated
                     intimin receptor Tir"
                     /protein_id="HGU4550729.1"
                     /translation="MPIGNLGHNPNVRALIPPAPPLPSQTDGAGGARNQLINSNGPMG
                     SRLLFTPIRNSVADAADSRASDIPGLPTNPLRFAASEVSLHGALEVLHDKGGLDTLNS
                     AIGSSLFRVETRDDGSHVAIGQKNGLETTVVLSEQEFSSLQSLDPEGKNKFVFTGGRG
                     GAGHAMVTVASDIAEARQRIIDKLEPKDTKETKEPGDPNSGEGKIIEIHTSTSTSSLR
                     ADPKLWLSLGTIAAGLIGMAATGIAQAVALTPEPDDPITTDPDAAANTAEAAAKDQLT
                     KEAFQNPDNQKVNIDENGNAIPSGELKDDVVAQIAEQAKAAGEQARQEAIESNSQAQQ
                     KYDEQHAKREQEMSLSSGVGYGISGALILGGGIGAGVTAALHRKNQPAEQTITTRTVV
                     DNQPTNNASAQGNTDTSGPEESPASRRNSNASLASNGSDTSSTGTVENPYADVGMPRN
                     DSLARISEEPIYDEVAADPNYSVIQHFSGNSPVTGRLVGTPGQGIQSTYALLASSGGL
                     RLGMGGLTGGGESAVSTANAAPTPGPARFV"
BASE COUNT          459 a          374 c          434 g          389 t
ORIGIN      
        1 tttacataaa ataaaaagga gatattttat gcctattggt aatcttggcc acaatcccaa
       61 tgtgagagct ttaattccac ctgcaccgcc attaccttca caaaccgacg gtgcaggagg
      121 tgcccgtaat cagctcatta actcaaatgg cccgatgggg tctcgtttgc tatttacgcc
      181 tataaggaat tctgttgctg atgctgctga ttctcgtgcc agtgatattc ccggacttcc
      241 tacaaatcca ctgcgctttg ctgcgtccga ggtatctttg catggtgcgc ttgaagttct
      301 tcatgataaa ggggggcttg atactcttaa ctctgctatt ggatcttcgt tattccgtgt
      361 tgaaactcgg gatgatggca gccatgttgc tatcgggcaa aaaaatggcc tcgagaccac
      421 tgttgtttta agtgagcaag agttttctag cttacagtcc cttgatcctg aaggtaaaaa
      481 caaatttgta tttactggag gccgcggtgg cgcagggcat gctatggtca cggttgcttc
      541 agatatcgcc gaagcccgtc agaggataat agataaatta gaaccaaagg atacaaagga
      601 gacgaaggag ccaggggatc caaatagtgg cgagggaaaa atcattgaaa ttcatacctc
      661 aacctcaact tctagcctcc gtgcagatcc taaactttgg ttgtcattgg ggactattgc
      721 tgcaggtctg atagggatgg ctgcgacggg gattgcacag gctgttgcgt tgactccaga
      781 gccggatgac ccaatcacta ccgaccctga tgctgcagca aacacagctg aagcagcggc
      841 aaaagatcag ttaacgaaag aagcattcca gaacccagat aaccagaaag ttaatatcga
      901 tgagaacgga aatgcaattc cgtccgggga actaaaagat gatgttgttg cgcaaatagc
      961 agaacaagct aaagcggcgg gtgaacaggc cagacaggaa gctattgaaa gtaattctca
     1021 ggcgcagcaa aaatatgatg aacagcatgc taaacgcgaa caggaaatgt ctctttcatc
     1081 gggggttggc tacggtatta gtggtgcgct gattcttggc gggggaattg gtgccggtgt
     1141 tactgctgct cttcatcgga aaaaccaacc ggcagaacaa acaatcacta cacgtacggt
     1201 agtcgataat cagcctacga ataacgcatc tgcgcagggc aatactgaca caagtgggcc
     1261 agaagagtcc ccggcgagca gacgtaattc gaatgccagc ctcgcatcga acgggtctga
     1321 cacctccagc acgggcacgg tagagaatcc gtatgctgac gttggaatgc ccagaaatga
     1381 ttcactggct cgcatttcag aggaacctat ttatgatgag gtcgctgcag atcctaatta
     1441 tagcgtcatt caacattttt cagggaacag cccagttacc ggaaggttag tgggaacccc
     1501 agggcaaggt atccaaagta cttatgcgct tctggcaagc agcggcggat tgcgtttagg
     1561 tatgggagga ttaacggggg ggggcgagag cgcagtaagt actgccaatg ccgcaccaac
     1621 gccgggaccc gcacgtttcg tttaaatata tccgtg
//
LOCUS       DAZKHF020000475         1645 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2201S31241
            SAMN43267800-rid25106413.denovo.478, whole genome shotgun sequence.
ACCESSION   DAZKHF020000475 DAZKHF020000000
VERSION     DAZKHF020000475.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267800
            Sequence Read Archive: SRR30307263
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1645)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1645)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 1645)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350581.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350581.2
            Genome Coverage       :: 151x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:21:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,652
            CDSs (total)                      :: 5,557
            Genes (coding)                    :: 5,320
            CDSs (with protein)               :: 5,320
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 237
            CDSs (without protein)            :: 237
            Pseudo Genes (ambiguous residues) :: 0 of 237
            Pseudo Genes (frameshifted)       :: 102 of 237
            Pseudo Genes (incomplete)         :: 130 of 237
            Pseudo Genes (internal stop)      :: 52 of 237
            Pseudo Genes (multiple problems)  :: 41 of 237
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1645
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267800-rid25106413.denovo.478"
                     /serotype="O 26:H11"
                     /isolate="2201S31241"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-01"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            complement(1..1645)
                     /gene="cptA"
                     /locus_tag="ACHOSC_RS24885"
                     /old_locus_tag="pgaptmp_004977"
                     /pseudo
     CDS             complement(1..1645)
                     /gene="cptA"
                     /locus_tag="ACHOSC_RS24885"
                     /old_locus_tag="pgaptmp_004977"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418390.1"
                     /note="frameshifted; incomplete; missing N-terminus and
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="phosphoethanolamine transferase CptA"
BASE COUNT          484 a          305 c          362 g          494 t
ORIGIN      
        1 ggtatcatag tcagttaacg catttttctt ataggggttt cctatccagc gagtgatttc
       61 tttgaaatgc tggtttaata tggaacgctc tggctcatag ttatcataac gtaaaccagc
      121 caaatccgac caggtatgga taaaatcagc cagactgtat ttacggttaa tgtactcaga
      181 aaatgtatga tgtcgattaa gtagccattt ttcagaggcc cagagaataa atggaatatt
      241 gtacataggt cgggttgggg tgtcttcgtt acgtcccagc attttatgtg gtggcgtatc
      301 gaatacttcc tctccatggt cagaaaaata gaaaagaaaa ccatcagggg ctgttgcttt
      361 gaaagttttg atcaacgttg caacaacatg gtcattaaat atattagcat tatcgtaatc
      421 gttatataat tccagttcat tatcagtaag tccttctggt gcatgttccc tgtccccatc
      481 gaatactccc tgaccttcag gataacggta tttatatttg atgtgtgttc ccaacaagtg
      541 aataataaca aattttttgg gagctggatc tgccagtact tcactaaacg gagccagtac
      601 gttagtatcg tactcacggg cactttgcgt tctctgctga ttcatgtagt actgtttatc
      661 tgtttgccga gagaagactg tcagcattgt gttgcgtgca gtcatcgttt gttgattggt
      721 gatccagaat gttttgtaac cggcctgttt cattatattc atcagggaag gttgtgtcag
      781 gtagagttcg ggctgctgtt catcagcgaa agtcaatacc tgctgtagta cttcgatggt
      841 atatggtctt gaggagacaa cattattaaa cacaaacata ttttgttccg ttttgtataa
      901 ttcatctagt ccgggggttg tttttctggg atatccatac agactcattc tcccacgctg
      961 ggttgattct cctatcacca gaaccagtgt tcgtggggta tctccggaaa tatcgttaaa
     1021 gtttttcaga gcggggagcg cattattttc ctccagcaaa cggttcagag aattaagctg
     1081 gagacggtac tgataatatc cggtaataaa ttgccagggg gcagcgggct ccatacggga
     1141 tgccaggcgt cccagagtat tgtcaatagt cctgttttta acaacagtat ttattaatac
     1201 agggtgcaga attaaaccgt agataataaa taaaggtata atgtagcgcc agtatgatgg
     1261 tatatatatc gggctaagac gagtccatag cagaatagca atagctgtat atattgtcga
     1321 tataaatagt atttttaaac taaaatactg actaaaatat tcgctggctt cacttgtatt
     1381 actttcaaac atgacgaaca gtacactctg agagaattcc tgcccgtaga ttagataata
     1441 gcaaagggca gcaagagatg tgccccataa aaccacgccg attaatgctg ctgtaacctt
     1501 tattctctct gggaaaagaa aaacagggat taaccagaga gagctgaaaa gtagcgagtc
     1561 acgaagtcca ttggtgccac tatatccagt cagacaaatg ataatctgaa gtaaagtgga
     1621 aaaaatacca gaaataaatg agagc
//
LOCUS       DAZKHF020000476         1643 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2201S31241
            SAMN43267800-rid25106413.denovo.479, whole genome shotgun sequence.
ACCESSION   DAZKHF020000476 DAZKHF020000000
VERSION     DAZKHF020000476.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267800
            Sequence Read Archive: SRR30307263
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1643)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1643)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 1643)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350581.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350581.2
            Genome Coverage       :: 151x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:21:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,652
            CDSs (total)                      :: 5,557
            Genes (coding)                    :: 5,320
            CDSs (with protein)               :: 5,320
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 237
            CDSs (without protein)            :: 237
            Pseudo Genes (ambiguous residues) :: 0 of 237
            Pseudo Genes (frameshifted)       :: 102 of 237
            Pseudo Genes (incomplete)         :: 130 of 237
            Pseudo Genes (internal stop)      :: 52 of 237
            Pseudo Genes (multiple problems)  :: 41 of 237
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1643
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267800-rid25106413.denovo.479"
                     /serotype="O 26:H11"
                     /isolate="2201S31241"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-01"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            complement(<1..337)
                     /locus_tag="ACHOSC_RS24890"
                     /old_locus_tag="pgaptmp_004978"
     CDS             complement(<1..337)
                     /locus_tag="ACHOSC_RS24890"
                     /old_locus_tag="pgaptmp_004978"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C40 family peptidase"
                     /protein_id="HGU4550730.1"
                     /translation="MTQTESAILAHARRCVPAESCGFVVRTPEGERYIPCVNISAEPE
                     AYFRIAPEDWLRAEMQGEIVALVHSHPGGLPWLSEADRRLQIKSALSWWLVCRGDIHK
                     FRCVPHLTGR"
     gene            complement(348..1046)
                     /locus_tag="ACHOSC_RS24895"
                     /old_locus_tag="pgaptmp_004979"
     CDS             complement(348..1046)
                     /locus_tag="ACHOSC_RS24895"
                     /old_locus_tag="pgaptmp_004979"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030430 - host cell cytoplasm [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0046718 - symbiont entry into host cell
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage minor tail protein L"
                     /protein_id="HGU4550731.1"
                     /translation="MQHIHGESLNESVKSEQSPRVVLWEIDLKAQGGERYFFCNELNE
                     KGEPVTWQGRKYQAYPIDGSGFEMNGKGSSARPSLTVSNLFGLVTGMAEDLQSLVGAT
                     VVRRRVYARFLDAVNFVAGNPEADPEQELSDRWVVEQMSELTAMTASFVLATPTETDG
                     ALFPGRIMLANTCMWDYRGDECGYNGPAVADEFDNPTTDIRKDRCSKCMRGCEMRGMV
                     ANFGGFLSINKLSQ"
     gene            complement(1046..1387)
                     /locus_tag="ACHOSC_RS24900"
                     /old_locus_tag="pgaptmp_004980"
     CDS             complement(1046..1387)
                     /locus_tag="ACHOSC_RS24900"
                     /old_locus_tag="pgaptmp_004980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HGU4550732.1"
                     /translation="MAEIKTLHLVPREGMQVSEKPSVARVRFGDGYEQRRPTGLNPQL
                     KTFQAVFRVTDESTRRWLDEFLSWHGGYRAFLWRPPKHNRTVRVVCREWSVTDNVRYS
                     DFSCTIEQVVN"
     gene            complement(1380..>1643)
                     /locus_tag="ACHOSC_RS24905"
                     /old_locus_tag="pgaptmp_004981"
     CDS             complement(1380..>1643)
                     /locus_tag="ACHOSC_RS24905"
                     /old_locus_tag="pgaptmp_004981"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tape measure protein"
                     /protein_id="HGU4550733.1"
                     /translation="GLMGEAGPEAIMPLTRAADGSLGVRAVGSMNGSAGLVYSPVYHI
                     AIQNDGTNGQIGPEAAGSLVQLIDQRVQAVMLSMRRDGGMLSG"
BASE COUNT          349 a          595 c          375 g          324 t
ORIGIN      
        1 gccgtcctgt cagatgtggc acacagcgga atttatgaat gtccccccgg cagaccagcc
       61 accaggacag tgcacttttt atctgcagcc gccggtcggc ctcgctcagc cagggcagac
      121 caccgggatg actgtggacc agtgccacaa tctccccctg catctctgcc cgcagccagt
      181 cttccggtgc aatacgaaaa tacgcctccg gctctgcaga gatattcaca caagggatat
      241 accgctcccc ctccggcgtt ctcaccacga agccacacga ctccgcaggc acacaccgcc
      301 gggcatgcgc cagaatcgct gattcagtct gtgtcataaa ccgggattta ctgcgaaagt
      361 ttattaatgg aaaggaaacc gccaaaatta gccaccatgc cgcgcatctc acacccgcgc
      421 atgcacttgc tgcatctgtc cttacggata tccgtagtgg ggttgtcgaa ctcatccgcc
      481 accgcaggac cgttataccc gcattcatct ccccggtaat cccacataca ggtgttcgcc
      541 agcatgatgc gaccaggaaa cagcgccccg tccgtctcgg tcggtgttgc cagcacaaac
      601 gaggctgtca tggccgtcag ctctgacatc tgctccacca cccagcggtc gctcagctcc
      661 tgctccgggt ccgcttccgg attgcccgcc acaaaattca ccgcatccag aaaacgggca
      721 tacacccggc ggcggaccac cgtggccccc accaggctct gcaggtcctc cgccatcccg
      781 gtgaccagac caaacagatt cgacaccgtc agcgacgggc gggcactgct gcccttcccg
      841 ttcatctcaa agccactgcc gtcaatcggg tatgcctggt acttccgccc ctgccaggtc
      901 accggctccc ctttttcatt cagctcatta cagaaaaaat accgctcacc accctgggcc
      961 ttcaggtcaa tttcccagag caccacccgc ggtgactgct ctgatttaac cgactcgttc
     1021 aggctttccc cgtgaatatg ctgcatcagt tcaccacctg ctcaatcgta cagctgaaat
     1081 cactgtaccg gacgttatcc gtgacgctcc actcccggca caccaccctc accgtccggt
     1141 tatgtttcgg cggtcgccac aaaaaggcac ggtaaccacc atgccaggat aaaaattcat
     1201 ccagccagcg ccgggttgac tcatccgtca cccggaacac cgcctgaaac gtcttcagtt
     1261 gaggattcag tcctgtgggg cgacgctgtt cataaccgtc accaaaccgt accctcgcca
     1321 ccgacggttt ctcactcacc tgcatccctt cacgcgggac cagatgcagc gtttttatct
     1381 cagccactca gcattcctcc gtcacgtcgc atggacaaca tcaccgcctg cacccgctgg
     1441 tcaatcagct gcacaagact gcctgccgcc tccggcccta tctggccatt agtcccgtca
     1501 ttctgaatgg cgatatggta gaccggggaa tacaccaggc ccgcactacc gttcatactg
     1561 cccaccgctc gcacacccag cgagccatcc gccgcccggg tcaggggcat aatggcttca
     1621 ggaccggcct cccccatcag tcc
//
LOCUS       DAZKHF020000477         1643 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2201S31241
            SAMN43267800-rid25106413.denovo.480, whole genome shotgun sequence.
ACCESSION   DAZKHF020000477 DAZKHF020000000
VERSION     DAZKHF020000477.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267800
            Sequence Read Archive: SRR30307263
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1643)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1643)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 1643)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350581.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350581.2
            Genome Coverage       :: 151x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:21:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,652
            CDSs (total)                      :: 5,557
            Genes (coding)                    :: 5,320
            CDSs (with protein)               :: 5,320
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 237
            CDSs (without protein)            :: 237
            Pseudo Genes (ambiguous residues) :: 0 of 237
            Pseudo Genes (frameshifted)       :: 102 of 237
            Pseudo Genes (incomplete)         :: 130 of 237
            Pseudo Genes (internal stop)      :: 52 of 237
            Pseudo Genes (multiple problems)  :: 41 of 237
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1643
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267800-rid25106413.denovo.480"
                     /serotype="O 26:H11"
                     /isolate="2201S31241"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-01"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            99..1637
                     /locus_tag="ACHOSC_RS24910"
                     /old_locus_tag="pgaptmp_004982"
     CDS             99..1637
                     /locus_tag="ACHOSC_RS24910"
                     /old_locus_tag="pgaptmp_004982"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000099160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS66-like element ISEc22 family transposase"
                     /protein_id="HGU4550734.1"
                     /translation="MSSSLPDDINALKRLLAEQEALNRALLEKLNEREREIDHLQAQL
                     DKLRRMNFGSRSEKVSRRIAQMEADLKALQKESDTLTGRVDDPAVQRPLRQTRTRKPF
                     PESLPRDEKRLLPAASCCPECGGSLSYLGEDAAEQLELMRSAFRVIRTVREKHACTQC
                     DAIVQAPAPSRPIERGIAGPGLLARVLISKYAEHTPLYRQSEMYGRQGVELSRSLLSG
                     WVDACCRLLSPLEEALQDYVLTDGKLHADDTPVPVLLPGNKKTKTGRLWTYVRDDRNA
                     GSTLAPAVLFAYSPDRKGIHPQTHLAGFSGVLQADAYAGFNELYRDGRITEAACWAHA
                     RRKIHDVHVRTPSALTEEALKRIGELYAIEAEIRGMTAEQRLAERQLKTKPLLKSLES
                     WLREKMKTLSRHSELAKAFAYALNQWPALTYYADDGWAEADNNIAENALRMVSLGRKN
                     YLFFGSDHGGERGALLYSLIGTCKLNGVEPESYLRYVLDVIADWPINRVGELLPWRVA
                     LPTE"
BASE COUNT          371 a          472 c          497 g          303 t
ORIGIN      
        1 tcgactggcg gcagcctaaa agactgctta cgtccctgac tatgttgtaa gcctctttat
       61 cctggtcgac gctgaatgag cctggtaata tacccggtat gagcagctca cttcctgacg
      121 atatcaatgc actgaaacgt ctccttgccg aacaggaggc gctgaaccgt gccctgctgg
      181 aaaagctgaa cgagcgtgaa cgcgaaatag accatctgca ggcacagctg gataagctgc
      241 gccggatgaa cttcggcagc cgctccgaaa aagtctcccg tcgtatcgca cagatggaag
      301 ctgacctgaa ggcacttcag aaagaaagtg atacccttac cggtcgggtt gacgacccgg
      361 ccgtgcagcg cccgctgcgt caaacccgca cccgcaaacc gttccccgaa tcactccccc
      421 gcgatgaaaa acggctgctg ccggcagcat catgctgccc ggaatgtgga ggctcactga
      481 gctatctggg tgaggatgcc gccgaacagc tggagttgat gcgcagcgcc ttccgggtta
      541 tccggactgt acgtgaaaag catgcctgta ctcagtgcga tgccatcgtg caggcccccg
      601 cgccttcacg gcccatcgag cggggtatcg caggaccggg gctgctggcc cgcgtgctga
      661 tctcaaagta tgcagagcac accccgctgt accgccagtc tgaaatgtac ggccgccagg
      721 gcgtggagct gagtcgttca ctgctgtcgg gctgggtgga tgcatgctgc cggctactgt
      781 caccgctgga agaagcgctt caggactatg tgctgactga cggtaagctc catgctgatg
      841 acacgcctgt cccggtgctg ttgccaggca ataagaaaac gaagaccggg cggttatgga
      901 cctacgttcg tgacgaccgt aacgccgggt caacgctggc gccggcggtg ttgttcgctt
      961 acagcccgga cagaaaaggc atccatccgc agacccatct tgcggggttc agtggtgtac
     1021 tgcaggcgga tgcatacgcc gggttcaacg agctgtaccg ggatggccgg ataacggaag
     1081 ccgcctgttg ggctcacgcc cgccgtaaaa tccacgatgt gcacgttcgc accccgtcag
     1141 ccctgacgga ggaagcgctg aaacggatcg gcgaactgta cgccatcgag gcagagataa
     1201 ggggaatgac ggcggagcag cgccttgccg aacgtcagtt gaaaacgaaa ccgctgctga
     1261 aatccctgga aagctggctg cgtgaaaaga tgaaaaccct gtcgcgacac tcagaactgg
     1321 cgaaagcgtt cgcatacgcc ctgaaccagt ggccggcgct gacgtactat gcagatgatg
     1381 gctgggctga ggcggacaat aacatcgctg aaaatgcgtt gcggatggtc agtctgggcc
     1441 gcaaaaacta cctgttcttc ggttcggatc atggaggaga gcggggagcg ctgctgtaca
     1501 gcctgatcgg gacgtgcaaa ctgaacggag tggagccaga aagctacctc cgctatgtcc
     1561 ttgacgtcat agccgactgg ccgataaacc gggtcggcga actgctcccc tggcgcgtag
     1621 cactgccgac tgaataacac atc
//
LOCUS       DAZKHF020000478         1638 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2201S31241
            SAMN43267800-rid25106413.denovo.481, whole genome shotgun sequence.
ACCESSION   DAZKHF020000478 DAZKHF020000000
VERSION     DAZKHF020000478.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267800
            Sequence Read Archive: SRR30307263
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1638)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1638)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 1638)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350581.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350581.2
            Genome Coverage       :: 151x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:21:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,652
            CDSs (total)                      :: 5,557
            Genes (coding)                    :: 5,320
            CDSs (with protein)               :: 5,320
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 237
            CDSs (without protein)            :: 237
            Pseudo Genes (ambiguous residues) :: 0 of 237
            Pseudo Genes (frameshifted)       :: 102 of 237
            Pseudo Genes (incomplete)         :: 130 of 237
            Pseudo Genes (internal stop)      :: 52 of 237
            Pseudo Genes (multiple problems)  :: 41 of 237
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1638
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267800-rid25106413.denovo.481"
                     /serotype="O 26:H11"
                     /isolate="2201S31241"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-01"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            115..294
                     /locus_tag="ACHOSC_RS24915"
                     /old_locus_tag="pgaptmp_004983"
     CDS             115..294
                     /locus_tag="ACHOSC_RS24915"
                     /old_locus_tag="pgaptmp_004983"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309858.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006950 - response to stress [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="general stress protein"
                     /protein_id="HGU4550735.1"
                     /translation="MAEHRGGSGNFAEDREKASDAGRKGGQHSGGNFKNDPQRASEAG
                     KKGGQQSGGNKSGKS"
     gene            380..880
                     /locus_tag="ACHOSC_RS24920"
                     /old_locus_tag="pgaptmp_004984"
     CDS             380..880
                     /locus_tag="ACHOSC_RS24920"
                     /old_locus_tag="pgaptmp_004984"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferritin-like domain-containing protein"
                     /protein_id="HGU4550736.1"
                     /translation="MNMKTIEDVFIHLLSDTYSAEKQLTRALAKLARATSNEKLSQAF
                     HAHLEETHGQIERIDQVVESESNLKIKRMKCVAMEGLIEEANEVIESTEKNEVRDAAL
                     IAAAQKVEHYEIASYGTLATLAEQLGYRKAAKLLKETLEEEKATDIKLTDLALNNVNK
                     KAENKA"
     gene            926..1432
                     /locus_tag="ACHOSC_RS24925"
                     /old_locus_tag="pgaptmp_004985"
     CDS             926..1432
                     /locus_tag="ACHOSC_RS24925"
                     /old_locus_tag="pgaptmp_004985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferritin-like domain-containing protein"
                     /protein_id="HGU4550737.1"
                     /translation="MNRIEHYHDWLRDAHAMEKQAESMLESMASRIDNYPELRARIEQ
                     HLSETKNQIVQLETILDRNDISRSVIKDSMSKMAALGQSIGGIFPSDEIVKGSISGYV
                     FEQFEIACYTSLLAAAKNAGDTVSIPIIEAILNEEKQMADWLIQHIPQTTEKFLIRSE
                     TDGVEAKK"
     gene            complement(1492..1623)
                     /locus_tag="ACHOSC_RS24930"
                     /old_locus_tag="pgaptmp_004986"
                     /pseudo
     CDS             complement(1492..1623)
                     /locus_tag="ACHOSC_RS24930"
                     /old_locus_tag="pgaptmp_004986"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415772.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="OmpW family outer membrane protein"
BASE COUNT          539 a          331 c          356 g          412 t
ORIGIN      
        1 tctatcttct cgttgcgctt aatttgacta attctcatta gcgactaatt ttaatgagtg
       61 tcgacacaca acactcatat taatgaaaca atgcaacgca acgggagaaa taacatggcc
      121 gaacatcgtg gtggttcagg aaatttcgcc gaagaccgtg agaaggcatc cgacgcaggc
      181 cgtaaaggcg gtcagcatag cggcggtaat tttaaaaatg atccgcaacg cgcatctgaa
      241 gcgggtaaaa aaggcggtca acaaagcggt ggtaataaat caggcaaatc ctgattcacc
      301 gttaattatt aatatcagct gataaagata attgcatctg cgggccgtat ggctcgcagt
      361 gatttcactg gagaaaaata tgaatatgaa gaccattgaa gatgtattta ttcacctgct
      421 ttcagatacc tacagcgcag aaaaacaatt aacccgggca ctggcaaaac tcgcaagagc
      481 aacatcaaat gaaaaattaa gtcaggcttt tcatgcgcac ctcgaggaaa ctcatggaca
      541 gattgaacgt attgatcaag ttgtggagtc cgaatcgaac ctgaaaatta agcgcatgaa
      601 atgtgtggca atggaaggtc ttattgaaga agctaatgag gtcatcgaaa gtaccgagaa
      661 aaacgaagtg cgtgatgccg cactgattgc cgcagcgcag aaagtcgagc attatgagat
      721 tgccagttac gggacattag cgacgctggc tgaacaatta ggttaccgta aagcagcgaa
      781 gcttctgaaa gaaaccctgg aagaagaaaa ggccaccgac atcaaactga ctgatctggc
      841 ccttaataac gtaaataaga aagccgaaaa taaagcctga aatatgaatt ttaactttta
      901 gtcattttat aaagaggaca ttttcatgaa tcgtattgaa cattatcatg actggttacg
      961 tgacgcccac gcaatggaaa agcaagccga atctatgctt gaatccatgg ccagccgtat
     1021 agataattat cctgaactac gcgctcgtat tgaacaacat cttagtgaaa ccaaaaacca
     1081 gattgttcaa ctggaaacta ttcttgatcg taatgacatt tcacgttcag tcattaaaga
     1141 ttccatgagt aaaatggctg cgcttgggca gtcaatcggt ggtatattcc cttctgatga
     1201 aatagttaaa ggctctatta gcggatatgt cttcgagcaa tttgaaatcg cctgttacac
     1261 ctcactatta gcagcagcaa aaaatgccgg tgatacagtc tcaattccga tcatcgaagc
     1321 gattttaaat gaggaaaagc aaatggccga ctggctgatt cagcatattc cgcaaacaac
     1381 tgagaaattt ttaattcgtt ctgaaactga tggcgtagaa gcgaagaaat aatacccttt
     1441 ataccatgtc cttattgacc ccgtatatta cggggtcgtt tttgtgcgga attaaaaacg
     1501 atatcctgct gagaacataa acacccacgg atcgaggcgt acgctatcgt gttgctgtgc
     1561 accgcctagc ttataattgg cggtggtatc gatatccatg taccacactg gtagatttta
     1621 caaaactcaa taacatat
//
LOCUS       DAZKHF020000479         1636 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2201S31241
            SAMN43267800-rid25106413.denovo.482, whole genome shotgun sequence.
ACCESSION   DAZKHF020000479 DAZKHF020000000
VERSION     DAZKHF020000479.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267800
            Sequence Read Archive: SRR30307263
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1636)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1636)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 1636)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350581.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350581.2
            Genome Coverage       :: 151x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:21:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,652
            CDSs (total)                      :: 5,557
            Genes (coding)                    :: 5,320
            CDSs (with protein)               :: 5,320
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 237
            CDSs (without protein)            :: 237
            Pseudo Genes (ambiguous residues) :: 0 of 237
            Pseudo Genes (frameshifted)       :: 102 of 237
            Pseudo Genes (incomplete)         :: 130 of 237
            Pseudo Genes (internal stop)      :: 52 of 237
            Pseudo Genes (multiple problems)  :: 41 of 237
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1636
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267800-rid25106413.denovo.482"
                     /serotype="O 26:H11"
                     /isolate="2201S31241"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-01"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            <1..202
                     /locus_tag="ACHOSC_RS24935"
                     /old_locus_tag="pgaptmp_004987"
     CDS             <1..202
                     /locus_tag="ACHOSC_RS24935"
                     /old_locus_tag="pgaptmp_004987"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016239865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4550738.1"
                     /translation="ELIFSALRILGAMWMVATFIVVASSFVRLVGEGKDLVGVLFGSI
                     FLWVIIGVAPVAVAKMAWRFVS"
     gene            210..656
                     /locus_tag="ACHOSC_RS24940"
                     /old_locus_tag="pgaptmp_004988"
     CDS             210..656
                     /locus_tag="ACHOSC_RS24940"
                     /old_locus_tag="pgaptmp_004988"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination protein NinB"
                     /protein_id="HGU4550739.1"
                     /translation="MKQIYMLRNEAIRNNAIDAILSLPIDDKSPHEVHIKEPKRSNPQ
                     NRLMWALLQDVSRQVLWHGQRLAPEDWKDLFTALWLKTKKLEQRSVPGIDGGVVMLGV
                     RTSKMRKASMTELIEIMFWFGSERNVRWSDDSRREYEWSQRKGRAA"
     gene            653..1180
                     /locus_tag="ACHOSC_RS24945"
                     /old_locus_tag="pgaptmp_004989"
     CDS             653..1180
                     /locus_tag="ACHOSC_RS24945"
                     /old_locus_tag="pgaptmp_004989"
                     /EC_number="2.1.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0009007 - site-specific
                     DNA-methyltransferase (adenine-specific) activity
                     [Evidence IEA];
                     GO_process: GO:0009307 - DNA restriction-modification
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage N-6-adenine-methyltransferase"
                     /protein_id="HGU4550740.1"
                     /translation="MTIKSNTPAHDKDCWQTPLWLFDALDIEFGFWLDSAASDKNALC
                     AHWLTEADDALNSEWISHGAIWNNPPYSNIRPWVEKAAEQCIQQRQTVVMLVPEDMSV
                     GWFSKALESVDEVRIITDGRINFIEPSTGLEKKGNSKGSMLLIWRPFISPRRMFTTVS
                     KAALMAIGQGVRRAA"
     gene            1177..1359
                     /locus_tag="ACHOSC_RS24950"
                     /old_locus_tag="pgaptmp_004990"
     CDS             1177..1359
                     /locus_tag="ACHOSC_RS24950"
                     /old_locus_tag="pgaptmp_004990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016063117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NinE family protein"
                     /protein_id="HGU4550741.1"
                     /translation="MRRQRRSFTDIICENCKYLPTKRYRNKRKPIPKESDVKTFNYTA
                     HLWDIRWLRHRARKTR"
BASE COUNT          440 a          344 c          451 g          401 t
ORIGIN      
        1 tgagttgatt ttttctgcat tgaggattct cggtgctatg tggatggtgg cgacgttcat
       61 tgtggttgcc agcagttttg tccggctggt aggcgaaggt aaagacctgg ttggtgtgct
      121 tttcggtagc attttcctgt gggtgattat cggtgttgcg cctgtcgctg tagcaaaaat
      181 ggcgtggcgt tttgtgagtt gaggtgacaa tgaagcaaat atacatgctt cgcaacgaag
      241 caatcagaaa caacgccata gacgcaatac tctcacttcc gatcgacgac aagtcacctc
      301 acgaagtcca cattaaagaa ccaaagcgga gcaatcctca aaaccgcctt atgtgggcgt
      361 tattgcagga cgtatcacgt caggtgcttt ggcatggaca gagacttgcg ccggaggact
      421 ggaaagacct gttcactgcc ctgtggctta agaccaaaaa actggagcaa cgaagtgtgc
      481 ctggtatcga tggtggcgtt gtcatgcttg gcgtgcgtac cagcaaaatg cgaaaggcca
      541 gcatgactga gcttatcgaa atcatgtttt ggttcggctc agagcgcaac gtgcggtgga
      601 gtgatgactc ccggcgagag tatgaatggt cacaacgaaa aggtagggct gcatgactat
      661 caaatcaaat acaccagcac acgacaagga ctgctggcaa acgccgcttt ggctttttga
      721 tgcgctggat attgagtttg gattctggct ggattcagct gcgagcgaca aaaatgctct
      781 gtgcgctcac tggctaactg aggccgacga cgcactcaat tctgagtgga taagccacgg
      841 tgcaatctgg aataacccac cgtacagcaa tatcaggccg tgggtggaaa aagccgctga
      901 gcagtgcata caacagcgac agacggtagt gatgcttgtg ccagaggata tgtctgtcgg
      961 atggttcagc aaggctctgg agagtgttga cgaagttcgc attatcactg atggacggat
     1021 taattttatc gaaccatcga cagggctgga gaagaaggga aacagtaaag gctccatgct
     1081 gctgatttgg cgaccgttca tcagtcctcg acggatgttt actaccgtat ccaaagcggc
     1141 attgatggcg atcgggcagg gcgtcaggag ggcggcatga ggcgacagcg acgaagtttc
     1201 accgacatca tctgcgaaaa ctgcaaatac cttccaacga aacgctacag aaataaacgc
     1261 aagccaatcc caaaagaatc tgacgtaaaa accttcaatt acacggctca cctgtgggat
     1321 atccggtggc taagacatcg tgcgaggaaa acaaggtaat tgactaaaat cgaagttacg
     1381 aacaagaaag cgtcgagcgg gcttcagtgt acactgagtg gattctatct aggcttagtg
     1441 catacagaag attgctggta aaggacatgc caggcaaaac gatgaggact gatatttatg
     1501 aaaacatctg attttttact gttcttgcat gcggtacagg aggggctttg accgggcatt
     1561 ttatcgtgaa tattttcact tggtatttct ttggttttag agattacttc actcgatggg
     1621 ttttaaatag ttttcg
//
LOCUS       DAZKHF020000480         1632 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2201S31241
            SAMN43267800-rid25106413.denovo.483, whole genome shotgun sequence.
ACCESSION   DAZKHF020000480 DAZKHF020000000
VERSION     DAZKHF020000480.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267800
            Sequence Read Archive: SRR30307263
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1632)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1632)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 1632)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350581.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350581.2
            Genome Coverage       :: 151x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:21:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,652
            CDSs (total)                      :: 5,557
            Genes (coding)                    :: 5,320
            CDSs (with protein)               :: 5,320
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 237
            CDSs (without protein)            :: 237
            Pseudo Genes (ambiguous residues) :: 0 of 237
            Pseudo Genes (frameshifted)       :: 102 of 237
            Pseudo Genes (incomplete)         :: 130 of 237
            Pseudo Genes (internal stop)      :: 52 of 237
            Pseudo Genes (multiple problems)  :: 41 of 237
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1632
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267800-rid25106413.denovo.483"
                     /serotype="O 26:H11"
                     /isolate="2201S31241"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-01"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            <1..1230
                     /locus_tag="ACHOSC_RS24955"
                     /old_locus_tag="pgaptmp_004991"
     CDS             <1..1230
                     /locus_tag="ACHOSC_RS24955"
                     /old_locus_tag="pgaptmp_004991"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RHS repeat-associated core domain-containing
                     protein"
                     /protein_id="HGU4550742.1"
                     /translation="RYLYDPLGRRVAKRVWRRERDLTGWMSLSRKPQVTWYGWDGDRL
                     TTIQNDRTRIQTIYQPGSFTPLIRVETATGEQAKTQRRSLADTLQQSGGEDGGSVVFP
                     PVLVQMLDRLESEILADRVSEESRRWLASCGLTVEQMQNQMDPVYTPARKIHLYHCDH
                     RGLPLALVSTEGATEWCAEYDEWGNLLNEENPHQLQQLIRLPGQQYDEESGLYYNRHR
                     YYDPLQGRYITQDPIGLKGGWNFYQYPLNPVQYIDSMGLASKYGHLNNGGYGARPNKP
                     PTPDPSKLPDIAKQLRLPYPIDQASSAPNVFKTFFRALSPYDYTLYCRKWVKPNLTCT
                     PQDDSQYPGMDTKTASDYLPQTNWPTTQLPPGYTCAEPYLFPDINKPDGPATAGIDDL
                     GEILAKMKQRTSRGIRK"
     gene            1227..>1632
                     /gene="ybfC"
                     /locus_tag="ACHOSC_RS24960"
                     /old_locus_tag="pgaptmp_004992"
     CDS             1227..>1632
                     /gene="ybfC"
                     /locus_tag="ACHOSC_RS24960"
                     /old_locus_tag="pgaptmp_004992"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YbfC"
                     /protein_id="HGU4550743.1"
                     /translation="MKRVLFFLLMIFVSFGVIADCEIQAKDHDCFTIFAKGTIFSAFP
                     VLNNKAMWRWYQNEDIGEYYWQTELGTCKNNKFTPSGARLLIRVGSLRLNENHAIKGT
                     LQELINTAEKTAFLGDRFRSYIRAGIYQKKSSD"
BASE COUNT          467 a          375 c          439 g          351 t
ORIGIN      
        1 cgctatcttt acgacccgct gggccgcagg gtggcaaaac gggtgtggcg acgtgaacgg
       61 gacctgacgg gctggatgtc gctgtcacgg aaaccgcaag tgacctggta cggctgggac
      121 ggcgaccgcc tgaccacaat acagaacgac agaacccgca tccagacgat ttatcagccg
      181 gggagcttca cgccactcat cagggttgaa accgccaccg gtgagcaggc gaaaacgcag
      241 cgccgcagcc tggcggatac ccttcagcag tccggcggcg aagacggtgg cagtgtggtg
      301 ttcccgccgg tgctggtgca gatgctcgac cggctggaaa gtgaaatcct ggctgaccgg
      361 gtgagtgagg aaagccgccg ctggctggca tcgtgcggcc tgacggtgga gcagatgcaa
      421 aaccagatgg acccggtgta cacgccggcg cgaaaaatcc acctgtacca ctgcgaccat
      481 cgcggcctgc cgctggcgct tgtcagcacg gaaggggcaa cagaatggtg cgcagaatac
      541 gatgaatggg gcaacctgct gaatgaagag aacccgcatc agctgcagca gcttatccgc
      601 ctgccggggc agcagtatga tgaggagtcc ggcctgtatt acaaccgcca ccgctattat
      661 gacccgctgc aggggaggta tatcactcag gatccgattg ggctgaaggg gggatggaat
      721 ttttatcagt atccgctgaa tccggttcag tatatagatt caatgggact ggcatcaaaa
      781 tatggacact taaataatgg cggatatgga gcgagaccca acaaaccgcc tacgcccgat
      841 ccaagtaaat tgccggacat agcgaaacaa ttaagactgc catatcctat tgaccaggcc
      901 agtagtgcgc ctaatgtttt caaaacattc ttcagagcat taagccctta cgactacaca
      961 ctgtattgca ggaagtgggt aaaaccaaat ctgacttgta cgccacagga tgattcccag
     1021 tatccaggga tggatacaaa gacagcaagt gattacctgc cacagacaaa ttggccaaca
     1081 actcaattac caccaggata tacttgtgca gaaccctatt tattcccaga cattaataaa
     1141 cccgatgggc cagcaacagc agggatagat gatttgggtg aaattttagc taagatgaaa
     1201 cagagaacat cgagaggaat aagaaaatga aaagagtttt gttctttttg ctgatgatat
     1261 ttgttagttt tggtgttata gctgattgcg aaatacaagc taaagatcat gattgtttta
     1321 ctattttcgc taagggtact attttttccg cgtttcctgt cttaaataat aaagccatgt
     1381 ggagatggta tcagaatgag gacatcggtg agtattattg gcaaacagaa ctgggtacat
     1441 gtaaaaataa taaattcaca ccaagcgggg cgagactctt aattcgcgtc gggtcactac
     1501 gccttaatga aaatcatgcc atcaaaggaa cgttgcagga gttgataaat acagcagaaa
     1561 aaacggcgtt tctcggcgat cggtttagaa gttatataag agcgggtatc tatcaaaaaa
     1621 agagcagtga tc
//
LOCUS       DAZKHF020000481         1624 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2201S31241
            SAMN43267800-rid25106413.denovo.484, whole genome shotgun sequence.
ACCESSION   DAZKHF020000481 DAZKHF020000000
VERSION     DAZKHF020000481.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267800
            Sequence Read Archive: SRR30307263
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1624)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1624)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 1624)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350581.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350581.2
            Genome Coverage       :: 151x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:21:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,652
            CDSs (total)                      :: 5,557
            Genes (coding)                    :: 5,320
            CDSs (with protein)               :: 5,320
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 237
            CDSs (without protein)            :: 237
            Pseudo Genes (ambiguous residues) :: 0 of 237
            Pseudo Genes (frameshifted)       :: 102 of 237
            Pseudo Genes (incomplete)         :: 130 of 237
            Pseudo Genes (internal stop)      :: 52 of 237
            Pseudo Genes (multiple problems)  :: 41 of 237
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1624
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267800-rid25106413.denovo.484"
                     /serotype="O 26:H11"
                     /isolate="2201S31241"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-01"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            complement(<1..677)
                     /locus_tag="ACHOSC_RS24965"
                     /old_locus_tag="pgaptmp_004993"
     CDS             complement(<1..677)
                     /locus_tag="ACHOSC_RS24965"
                     /old_locus_tag="pgaptmp_004993"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415949.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-guided endonuclease InsQ/TnpB family
                     protein"
                     /protein_id="HGU4550744.1"
                     /translation="MERLQAFKFQLRPGGQQEREMRRFAGACRFVFNRALARQNENHE
                     AGNKYIPYGKMASWLVEWKNATETQWLKDSPSQPLQQSLKDLERAYKNFFQNRAAFPR
                     FKKRGQNDAFRYPQGVKLDQENSRIFLPKLGWMRYRNSRQVTGVVKNVTVSQSCGKWY
                     ISIQTESEVSTPVHPSASMVGLDAGVAKLATLSDGTVFEPVNSFQKNQKTLARLQRQL
                     SRRVKFS"
     gene            685..1110
                     /gene="tnpA"
                     /locus_tag="ACHOSC_RS24970"
                     /old_locus_tag="pgaptmp_004994"
                     /pseudo
     CDS             685..1110
                     /gene="tnpA"
                     /locus_tag="ACHOSC_RS24970"
                     /old_locus_tag="pgaptmp_004994"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313209.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605 family transposase"
     gene            complement(1174..1467)
                     /locus_tag="ACHOSC_RS24975"
                     /old_locus_tag="pgaptmp_004995"
                     /pseudo
     CDS             complement(1174..1467)
                     /locus_tag="ACHOSC_RS24975"
                     /old_locus_tag="pgaptmp_004995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709992.3"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
     gene            complement(1398..>1624)
                     /locus_tag="ACHOSC_RS24980"
                     /old_locus_tag="pgaptmp_004996"
     CDS             complement(1398..>1624)
                     /locus_tag="ACHOSC_RS24980"
                     /old_locus_tag="pgaptmp_004996"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4550745.1"
                     /translation="KILISKIAHYGYNDAPGEKMTSTPSTIHSCCAVCNYSTLLVSVR
                     LLIIWLTSHKKPLRKLLILHGVIILLTLGF"
BASE COUNT          428 a          384 c          360 g          452 t
ORIGIN      
        1 ttgctgaatt tgaccctgcg gcttaactgt cgctgaagtc tcgccagcgt cttctggttt
       61 ttctgaaaac tgtttacagg ctcaaagact gtgccatctg acagcgtggc gagtttagcc
      121 acgccagcat ccagcccgac cattgatgct gaagggtgaa ccggagtgga tacttcactt
      181 tctgtctgaa tactgatgta ccacttaccg caggactggc tgacagtgac atttttcaca
      241 actcccgtga cctgacggct attccggtag cgcatccagc caagtttcgg cagaaaaata
      301 cggctgtttt cctgatcgag cttaacgccc tgcgggtagc ggaatgcatc attctgtccc
      361 cgctttttga atcggggaaa agcagcccga ttctggaaga agtttttgta agcccgctca
      421 aggtctttca gtgactgttg caatggctgt gagggcgaat ctttaagcca ttgcgtttca
      481 gtggcgtttt tccactcaac cagccaggaa gccattttcc cgtaagggat gtatttatta
      541 ccggcctcat gattctcatt ctgacgtgcc agagcacgat tgaaaacgaa acgacatgcg
      601 cctgcgaagc gcctcatttc acgctcctgt tgaccaccgg gtcttaactg gaatttaaat
      661 gcctgtagtc gttccacagg tcaaattata ttttggtctg tgaaaaaaga aaccgatatc
      721 cggcgtggaa ggcattgtgt tttcctgatg catgttcacc tggtctttgt caccaaatac
      781 cgacgccaga tttttgacca tgacgcgaca gaaaaactac gcacttactt ttcaaatgta
      841 tgtgctgatt ttgaagctga actggttgaa atggatggcg aaccagatca cgtccatttg
      901 ttaattagct atcctcccaa actggcgata tccagtctgg taaacagcct caaaggcgta
      961 tcgggtaggt tactgcgacg agatcgacca gatattgcag tcaggtatta ctacaaaggc
     1021 gttttgtgga gtcctggcta ttttgccaat agctgcggag gtgcgccaat atccgtcatc
     1081 cgccaataca ttgaacaaca gcaaacacct ggtcaggtgg aaaaccgcgc cttatatccc
     1141 cgccctgaag gacggggttt tacggcgcac cagataaacc aggccaagcg tatgctggtt
     1201 gatcaaataa tatcccccaa gcgcagcaat attaatattc acctgcaatg ggtcgatacc
     1261 tggcttaaaa atattgtatt tcttcccttc atccaataag gattccatta actgcaagtg
     1321 cgcatggtta atttctaaga gacgctgtga ttttgcataa tgcacacctt tactttgatt
     1381 ttcactatgc acaattttta aaaaccaagg gttagcaata taataacccc atgtaaaatc
     1441 aataattttc tcaatggctt tttgtggctg gttaaccaga tgattaacaa acgcaccgat
     1501 accaacagcg tagaataatt acataccgca caacaagaat gtatggtgga aggcgtggaa
     1561 gtcattttct ccccgggggc atcgttatat ccataatgag cgatttttga tattaatatc
     1621 tttc
//
LOCUS       DAZKHF020000482         1623 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2201S31241
            SAMN43267800-rid25106413.denovo.485, whole genome shotgun sequence.
ACCESSION   DAZKHF020000482 DAZKHF020000000
VERSION     DAZKHF020000482.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267800
            Sequence Read Archive: SRR30307263
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1623)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1623)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 1623)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350581.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350581.2
            Genome Coverage       :: 151x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:21:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,652
            CDSs (total)                      :: 5,557
            Genes (coding)                    :: 5,320
            CDSs (with protein)               :: 5,320
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 237
            CDSs (without protein)            :: 237
            Pseudo Genes (ambiguous residues) :: 0 of 237
            Pseudo Genes (frameshifted)       :: 102 of 237
            Pseudo Genes (incomplete)         :: 130 of 237
            Pseudo Genes (internal stop)      :: 52 of 237
            Pseudo Genes (multiple problems)  :: 41 of 237
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1623
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267800-rid25106413.denovo.485"
                     /serotype="O 26:H11"
                     /isolate="2201S31241"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-01"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            100..315
                     /gene="essD"
                     /locus_tag="ACHOSC_RS24985"
                     /old_locus_tag="pgaptmp_004997"
     CDS             100..315
                     /gene="essD"
                     /locus_tag="ACHOSC_RS24985"
                     /old_locus_tag="pgaptmp_004997"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416074.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage lysis protein EssD"
                     /protein_id="HGU4550746.1"
                     /translation="MKSMDKLTTGVAYGTSAGNAGFWALQLLDKVTPSQWAAIGVLGS
                     LVFGLLTYLTNLYFKIKEDRRKAARGE"
     gene            320..631
                     /gene="ydfR"
                     /locus_tag="ACHOSC_RS24990"
                     /old_locus_tag="pgaptmp_004998"
     CDS             320..631
                     /gene="ydfR"
                     /locus_tag="ACHOSC_RS24990"
                     /old_locus_tag="pgaptmp_004998"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdfR family protein"
                     /protein_id="HGU4550747.1"
                     /translation="MTQDYELVVKGVRNFENKVTVTVALQDKERFDGEIFDLDVAMDR
                     VEGAALEFYEAAARRSVRQVFLEVAEKLSEKVESYLQHQYSFKIENPANKHERPHHKY
                     I"
     gene            628..1161
                     /gene="rrrQ"
                     /locus_tag="ACHOSC_RS24995"
                     /old_locus_tag="pgaptmp_004999"
     CDS             628..1161
                     /gene="rrrQ"
                     /locus_tag="ACHOSC_RS24995"
                     /old_locus_tag="pgaptmp_004999"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysozyme"
                     /protein_id="HGU4550748.1"
                     /translation="MNTKIRYGLSAAVLALIGAGASAPQILDQFLDEKEGNHTMAYRD
                     GSGIWTICRGATVVDGKTVFPNMKLSKEKCDQVNAIERDKALAWVERNIKVPLTEPQK
                     AGIASFCPYNIGPGKCFPSTFYKRLNAGDRKGACEAIRWWIKDGGRDCRIRSNNCYGQ
                     VIRRDQESALTCWGIEQ"
     gene            1158..>1623
                     /gene="rzpQ"
                     /locus_tag="ACHOSC_RS25000"
                     /old_locus_tag="pgaptmp_005000"
     CDS             1158..>1623
                     /gene="rzpQ"
                     /locus_tag="ACHOSC_RS25000"
                     /old_locus_tag="pgaptmp_005000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2514 domain-containing protein"
                     /protein_id="HGU4550749.1"
                     /translation="MNQIFMVIFLVLSGFIVGNVWSDRGWQKKWAERDAAALSQEVNA
                     QFAARIIEQGRTIARDEAVKDAQQKSAEISARAAYLSDSVNQLRAEAKKYAIRLDAAK
                     HTADLAAAVRGKTTKTAEGMLTNMLGDIAAEAQLYAEIADERYIAGVTCQQIY"
BASE COUNT          456 a          340 c          429 g          398 t
ORIGIN      
        1 tttagcgccg ttgagaacaa cgtgtgctgt caaaactacc ccgtagactc cgatcttttt
       61 caaacatatt gcaccatccg tgtacatcgg ggtgaggata tgaaatcaat ggataagtta
      121 acaacaggtg ttgcctatgg cacatcggcg ggtaatgctg gtttctgggc attacagtta
      181 ctcgataaag taactccgtc acagtgggct gcaatcggtg tgctgggtag cctggttttt
      241 ggcctgctga cgtatctgac aaatctttat ttcaagatta aagaagacag gcgtaaggct
      301 gcgagaggag agtaatccaa tgactcaaga ctatgaactg gttgtgaaag gagtccgtaa
      361 ttttgagaat aaagttacgg taactgtagc cttacaggac aaggaacgct ttgacggtga
      421 aatttttgac ctggatgtcg ccatggaccg tgttgaagga gctgcgctgg agttttatga
      481 ggcagcagcc agaaggagcg tccggcaagt cttcctggaa gtagcagaaa aattgtcaga
      541 aaaagttgag tcttatctgc agcatcagta ctcctttaag attgaaaatc ctgccaataa
      601 gcacgagcgt cctcatcata aatatatatg aacacaaaaa tcagatacgg cctgtcggct
      661 gccgttctgg cgctgattgg tgctggcgca tctgctcctc agatacttga ccagtttctg
      721 gacgaaaaag aaggtaacca cacaatggca taccgcgatg gttctggcat atggaccatc
      781 tgtcggggtg ccacagtggt ggatggaaaa accgtttttc ccaatatgaa actgtcgaag
      841 gaaaaatgcg accaggtcaa cgccattgag cgtgataagg cgctggcatg ggtggagcgc
      901 aatattaaag taccactgac cgaaccacaa aaagcgggta tcgcgtcatt ttgtccctat
      961 aacattggcc ccggtaagtg tttcccgtcg acgttttata agcggctgaa tgctggtgat
     1021 cgtaaaggtg catgcgaagc gattcgctgg tggattaagg atggcggacg cgattgccgc
     1081 attcgttcaa ataactgtta cggtcaggtt attcgtcgtg accaggagag cgcattaacc
     1141 tgctggggga tagaacagtg aatcagatat tcatggtgat ttttctcgtg ttgtcaggat
     1201 ttatcgtcgg aaatgtctgg agcgacagag gatggcaaaa aaaatgggcg gaacgtgatg
     1261 ctgccgcatt atcacaagag gtaaatgctc aatttgctgc tcgaataatt gaacaggggc
     1321 gaactatagc ccgtgatgag gctgttaaag atgcacaaca gaaatctgct gaaatttctg
     1381 ccagggctgc ttatctgtct gatagtgtta accagttgcg tgccgaagca aaaaaatatg
     1441 ccatacgcct tgacgcagcg aagcataccg cagatcttgc cgctgccgtc agaggcaaaa
     1501 caaccaaaac cgccgaagga atgctcacca acatgctcgg agatattgca gcagaagctc
     1561 agctttatgc tgaaattgct gacgaacgct acatcgcagg agtgacttgt caacagatct
     1621 atg
//
LOCUS       DAZKHF020000483         1612 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2201S31241
            SAMN43267800-rid25106413.denovo.486, whole genome shotgun sequence.
ACCESSION   DAZKHF020000483 DAZKHF020000000
VERSION     DAZKHF020000483.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267800
            Sequence Read Archive: SRR30307263
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1612)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1612)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 1612)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350581.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350581.2
            Genome Coverage       :: 151x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:21:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,652
            CDSs (total)                      :: 5,557
            Genes (coding)                    :: 5,320
            CDSs (with protein)               :: 5,320
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 237
            CDSs (without protein)            :: 237
            Pseudo Genes (ambiguous residues) :: 0 of 237
            Pseudo Genes (frameshifted)       :: 102 of 237
            Pseudo Genes (incomplete)         :: 130 of 237
            Pseudo Genes (internal stop)      :: 52 of 237
            Pseudo Genes (multiple problems)  :: 41 of 237
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1612
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267800-rid25106413.denovo.486"
                     /serotype="O 26:H11"
                     /isolate="2201S31241"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-01"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            complement(<1..116)
                     /locus_tag="ACHOSC_RS25005"
                     /old_locus_tag="pgaptmp_005001"
     CDS             complement(<1..116)
                     /locus_tag="ACHOSC_RS25005"
                     /old_locus_tag="pgaptmp_005001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication protein"
                     /protein_id="HGU4550750.1"
                     /translation="MTNTAKILNFGRGNFAEQERNVADLDDGYARLSNMLLEA"
     gene            complement(149..448)
                     /locus_tag="ACHOSC_RS25010"
                     /old_locus_tag="pgaptmp_005002"
     CDS             complement(149..448)
                     /locus_tag="ACHOSC_RS25010"
                     /old_locus_tag="pgaptmp_005002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CII family transcriptional regulator"
                     /protein_id="HGU4550751.1"
                     /translation="MEQTSYSKLSQRDVDRAETDLLINLSTLTQRGLAKMIGCHESKI
                     SRTDWRFIASVLCAFGMASDISPISRAFKYALDGLTNKKRPVCKTERSEQIQMEF"
     gene            complement(587..820)
                     /locus_tag="ACHOSC_RS25015"
                     /old_locus_tag="pgaptmp_005003"
     CDS             complement(587..820)
                     /locus_tag="ACHOSC_RS25015"
                     /old_locus_tag="pgaptmp_005003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000064150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HGU4550752.1"
                     /translation="MSNELLRWRKEASSEEWKRLAALANTSVGYLDQIVYGFRRASPD
                     KANAIEEATRNFTGYKPVKKENLVFVSRRASAA"
     gene            934..>1612
                     /locus_tag="ACHOSC_RS25020"
                     /old_locus_tag="pgaptmp_005004"
     CDS             934..>1612
                     /locus_tag="ACHOSC_RS25020"
                     /old_locus_tag="pgaptmp_005004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000428099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HGU4550753.1"
                     /translation="MENKDIRKSNLAFLLDEHKKIAGNTNASFADKLGVSPSQLTQVS
                     GEKSTRNIGDKLARKFEAALGLPNGWLDLVHDVTPIASCSDSLTFVGQVRKGLVRVVG
                     EAILGVDGAIEMTEERDGWLNIYSDDPDAFGLRVKGDSMWPRIKSGEYVLIEPNTKVF
                     PGDEVFVRTVEGHNMIKVLGYDRDGEYQFTSINQDHRPITLPYHQVAKVEYVAGILKQ
                     SRHLDDIE"
BASE COUNT          446 a          337 c          371 g          458 t
ORIGIN      
        1 gcctcaagca gcatatttga tagtctggcg taaccatcat cgagatctgc cacattacgc
       61 tcctgttcgg caaagttacc tctgccgaag ttgagtattt ttgctgtatt tgtcataatg
      121 actcctgtgg attgatccag taattccctc agaattccat ctggatttgt tcagaacgct
      181 cggtcttgca caccgggcgt tttttattgg tgagtccatc aagcgcatac ttaaaagctc
      241 tgctaatcgg actgatgtct gatgccattc cgaaagcaca caagaccgaa gcaataaatc
      301 tccagtccgt tctgcttatc ttcgattcat gacagccaat catctttgcc agaccgcgct
      361 gggtaagcgt tgacaggttg ataagtaaat ctgtttctgc gcgatcaaca tcacgctgtg
      421 atagtttgct gtaacttgtt tgttccattt cttaagattt ccaatagtga atagctagtt
      481 gaaaggtatg cgtggaaacg catatggcct tagttggtca gatatcttgg aactcgcttt
      541 tcagcgacgt aggacgaatg tccgttgtta caaagagcgg ggttacttat gctgctgatg
      601 ctctacgcga tacgaacacc agattttcct ttttcacagg tttataaccc gtgaaattac
      661 gagtggcttc ttcgattgca ttcgctttat ctggggaagc tcttcgaaac ccatatacaa
      721 tctgatcaag atagccaact gaagtattcg ctaatgcggc gagtcgcttc cattcctcac
      781 tagaagcctc ttttcgccag cgtagtagtt cattactcat tagtgcctcc gtttatcaca
      841 cagagcgact ttaccatttt gataaataaa acgcaatgaa gatttatcaa aatgcatatt
      901 tatccatttg ctaaatagag ggagaatcgt gggatggaaa acaaagatat tcgcaaatcg
      961 aatctggcgt ttttgctaga tgagcataaa aaaatcgcgg gtaacactaa tgcaagcttt
     1021 gccgataagc ttggagttag cccttctcaa ctcacgcaag tctccggtga aaaaagcact
     1081 cgaaacatag gggataaact agcaagaaaa ttcgaagccg cacttgggtt acctaatggg
     1141 tggcttgatt tggtacatga tgtaacacca attgcatcat gctcagattc tttaactttt
     1201 gtcggtcagg taagaaaagg gttagtgcgc gtggttggtg aggcaattct tggtgttgat
     1261 ggtgccatcg agatgaccga agagcgcgat gggtggctca atatttatag cgatgatcca
     1321 gatgcctttg gtcttcgtgt gaaaggagac agcatgtggc ccagaataaa atcaggagaa
     1381 tatgtactca ttgagcctaa caccaaagta ttcccggggg atgaggtgtt tgtcagaact
     1441 gttgaaggac acaacatgat caaagttctt ggctatgaca gagatggaga ataccaattt
     1501 acaagcatca accaggacca caggccaata acgttgcctt atcatcaagt agcaaaggtg
     1561 gagtatgtgg ctggtattct gaagcaatct cgccatctgg atgacatcga gg
//
LOCUS       DAZKHF020000484         1610 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2201S31241
            SAMN43267800-rid25106413.denovo.487, whole genome shotgun sequence.
ACCESSION   DAZKHF020000484 DAZKHF020000000
VERSION     DAZKHF020000484.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267800
            Sequence Read Archive: SRR30307263
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1610)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1610)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 1610)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350581.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350581.2
            Genome Coverage       :: 151x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:21:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,652
            CDSs (total)                      :: 5,557
            Genes (coding)                    :: 5,320
            CDSs (with protein)               :: 5,320
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 237
            CDSs (without protein)            :: 237
            Pseudo Genes (ambiguous residues) :: 0 of 237
            Pseudo Genes (frameshifted)       :: 102 of 237
            Pseudo Genes (incomplete)         :: 130 of 237
            Pseudo Genes (internal stop)      :: 52 of 237
            Pseudo Genes (multiple problems)  :: 41 of 237
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1610
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267800-rid25106413.denovo.487"
                     /serotype="O 26:H11"
                     /isolate="2201S31241"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-01"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            <1..>1610
                     /locus_tag="ACHOSC_RS25025"
                     /old_locus_tag="pgaptmp_005005"
     CDS             <1..>1610
                     /locus_tag="ACHOSC_RS25025"
                     /old_locus_tag="pgaptmp_005005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000212852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tape measure protein"
                     /protein_id="HGU4550754.1"
                     /translation="TAFSDNAALFARSAASMREYGYSAGDVLKVTEAISTGLKISGAS
                     TAEAGSVITQFSQALAQGVLRGEEFNSVNESGDRIIRALAAGMGVARKDLKAMADDGK
                     LTADKVVPALISQLGVLRDEYAAMPETVSDGITKVENAFMAWVGGANEASGATKTLSG
                     VLNGVAGNIDNVATAAGALVAVGVARYFGNMAAGVGKATTELIAAKSGTVALAAAQAD
                     AAVQVQRKAAADALAAERAWQLAQAELALARNSSAEALATQNAVAKRRAMIVANAALV
                     QSNQAVAASRKALENATSVTGMLKSGAASLLSLVGGIPGLLMLGAGAWYTVYQNQEQA
                     RESARQYASTIDEIRAKVPGMTLPELADNEGKTREALEEQNRLIAEQESKVRGLKTQI
                     ADYQRWLQENPQGGSGVEIITHGLAEATGKLAVEQSRLTQMQEKAKSVQEALASLEYN
                     RVARIREEAAEQNRAYQALLQMNVQHTEFNRLLGLGNELLQQRQGLVNVPLRLPQATL
                     DDKQQSALTKTERELALSRLKGEEKERVR"
BASE COUNT          384 a          383 c          551 g          292 t
ORIGIN      
        1 acggcatttt cggataatgc ggccctgttt gcccgttcgg ctgcctcgat gcgtgaatat
       61 ggttacagtg ccggtgatgt actgaaggtg acggaggcca tttcgacagg gctgaaaata
      121 tccggtgcca gtacggcaga ggcgggctcg gtgatcaccc agttcagcca ggcgctggca
      181 cagggtgtgt tacggggtga ggaatttaat tcggtcaatg aaagcggaga ccggatcata
      241 cgtgcactgg ctgcgggtat gggcgtggcc cgtaaagacc ttaaggcgat ggcggacgac
      301 ggcaaactga cggcggataa agtcgttcct gcgttaatca gccagctggg ggtattgcgt
      361 gatgaatatg ccgccatgcc ggaaacggtc tctgacggga tcacaaaggt ggaaaacgcc
      421 tttatggcct gggtgggtgg cgcgaatgaa gccagcggag cgacgaaaac gctctccggc
      481 gtgctgaacg gtgttgccgg taatattgat aatgtggcaa cagccgcggg ggcgctggtt
      541 gccgtcgggg tggcccggta ctttggcaat atggccgcag gcgtgggaaa ggccaccaca
      601 gagctgatcg cggcgaagtc cgggacggtt gcccttgcag ccgcacaggc agatgcggct
      661 gttcaggtgc agcgtaaggc ggcagctgac gcgctggcgg cagaacgtgc ctggcaactg
      721 gcacaggctg aacttgcact ggccagaaat agcagtgcgg aggcgctggc cacacagaat
      781 gccgtcgcaa aacgccgggc catgattgtg gccaatgccg cactggtaca gtcgaatcag
      841 gcggtggcgg cctcgaggaa ggcgctggag aatgccacgt cggttacggg aatgctgaaa
      901 agtggcgcgg catccctgct ttcgcttgtc ggcgggatac ccggtttact gatgctggga
      961 gccggtgcct ggtacacggt gtaccagaat caggaacagg ccagggagtc cgcccgtcag
     1021 tatgcatcga cgattgatga aatccgggca aaggtaccgg gaatgaccct tccggaactg
     1081 gctgataatg agggtaaaac tcgcgaggca ctggaagagc agaatcgtct gattgctgag
     1141 caggaaagta aagtccgggg actgaaaaca cagattgctg actatcagcg ctggctgcag
     1201 gaaaatccac agggtggttc aggtgtggag attataaccc acggccttgc ggaggcaact
     1261 ggcaaactgg cggttgaaca gtcacgactg acacagatgc aggaaaaggc gaagtctgta
     1321 caggaagctc tggcgtcact ggaatacaac cgtgtggcga ggatacgcga ggaggcagca
     1381 gagcagaaca gagcatacca ggcattgctg cagatgaatg ttcagcatac ggaatttaac
     1441 cgtctgctgg ggctggggaa tgaactgctt cagcagcggc agggactggt gaatgtgccg
     1501 ttacggctgc cacaggccac cctggatgat aaacagcaga gtgccctgac aaaaacagag
     1561 cgtgagctgg ccctgtccag actgaaaggg gaagaaaaag agcgtgtccg
//
LOCUS       DAZKHF020000485         1585 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2201S31241
            SAMN43267800-rid25106413.denovo.488, whole genome shotgun sequence.
ACCESSION   DAZKHF020000485 DAZKHF020000000
VERSION     DAZKHF020000485.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267800
            Sequence Read Archive: SRR30307263
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1585)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1585)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 1585)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350581.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350581.2
            Genome Coverage       :: 151x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:21:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,652
            CDSs (total)                      :: 5,557
            Genes (coding)                    :: 5,320
            CDSs (with protein)               :: 5,320
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 237
            CDSs (without protein)            :: 237
            Pseudo Genes (ambiguous residues) :: 0 of 237
            Pseudo Genes (frameshifted)       :: 102 of 237
            Pseudo Genes (incomplete)         :: 130 of 237
            Pseudo Genes (internal stop)      :: 52 of 237
            Pseudo Genes (multiple problems)  :: 41 of 237
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1585
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267800-rid25106413.denovo.488"
                     /serotype="O 26:H11"
                     /isolate="2201S31241"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-01"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            complement(87..884)
                     /gene="mtfA"
                     /locus_tag="ACHOSC_RS25030"
                     /old_locus_tag="pgaptmp_005006"
     CDS             complement(87..884)
                     /gene="mtfA"
                     /locus_tag="ACHOSC_RS25030"
                     /old_locus_tag="pgaptmp_005006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310801.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DgsA anti-repressor MtfA"
                     /protein_id="HGU4550755.1"
                     /translation="MIKWPWKVQESAHQTALPWQEALSIPLLTGLTEQEQSKLVTLAE
                     RFLQQKRLVPLQGFELDSLRSCRIALLFCLPVLELGLEWLDGFHEVLIYPAPFVVDDE
                     WEDDIGLVHNQRIVQSGQSWQQGPIVLNWLDIQDSFDASGFNLIIHEVAHKLDTRNGD
                     RASGVPFISLREVAGWEHDLHAAMNNIQEEIELVGENAASIDAYAASDPAECFAVLSE
                     YFFSAPELFAPRFPSLWQRFCQFYQQDPLQRLHHANDTDSFSATNVH"
     gene            978..1067
                     /locus_tag="ACHOSC_RS25035"
                     /old_locus_tag="pgaptmp_005007"
     tRNA            978..1067
                     /locus_tag="ACHOSC_RS25035"
                     /old_locus_tag="pgaptmp_005007"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1012..1014,aa:Ser,seq:cga)
     gene            complement(1372..>1585)
                     /locus_tag="ACHOSC_RS25040"
                     /old_locus_tag="pgaptmp_005008"
     CDS             complement(1372..>1585)
                     /locus_tag="ACHOSC_RS25040"
                     /old_locus_tag="pgaptmp_005008"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4550756.1"
                     /translation="RTINTQLSHTDWQRKCFQEQVICHGKANQKYRLILVFKPFLLVS
                     MIQRLSKKLGNSTTLGNYGLCPAALI"
BASE COUNT          457 a          358 c          341 g          429 t
ORIGIN      
        1 gcgcatatta cttagcggta ccttgtctgt caacactaaa tttcataagc catttcaatt
       61 ggttaattaa tctgcaaagt tgttaattaa tgaacattcg tcgccgaaaa cgagtctgta
      121 tcattagcgt gatgcagtct ctgcaaagga tcttgttgat aaaattgaca gaaacgttgc
      181 cacaatgaag ggaaacgagg agcaaaaagt tctggggcgc taaagaaata ttcagaaagt
      241 acggcaaaac attcagcagg atcactggca gcataagcat caatgctcgc tgcattctca
      301 ccaactaatt cgatttcttc ctgaatgttg ttcattgcag catgaagatc gtgttcccag
      361 ccagcaacct cacgcaacga aataaaggga actccgctgg cgcgatcgcc gttacgggtg
      421 tccagcttat gagcgacttc atgaataatc aggttaaaac cagatgcatc aaaagaatct
      481 tgtatatcca accagttcaa aacgataggc ccttgctgcc agctctgacc tgactgaaca
      541 atacgttggt tatgcaccag accgatatcg tcttcccatt catcatcgac cacaaatggc
      601 gcaggataaa ttaagacttc atgaaaacca tccagccatt ccagtcctaa ctccagaacg
      661 ggtaggcaaa atagaagtgc tatccggcag cttcttaatg aatccagttc aaagccctgt
      721 aaaggaacaa gccgcttttg ctgtaaaaaa cgttcggcaa gagtgactaa tttgctttgt
      781 tcctgttccg tcagacccgt taaaaggggg atcgatagtg cttcctgcca gggaagggca
      841 gtttgatgtg ctgattcttg tactttccag ggccacttaa tcatcgtttt gctcgcaaac
      901 tcgtcacttg aacaaaattg cacggacagg gactgttaaa atgccaaatt tcctggcatc
      961 atggcaacca tctgaacgga gagatgccgg agcggctgaa cggaccggtc tcgaaaaccg
     1021 gagtaggggc aactctaccg ggggttcaaa tccccctctc tccgccactt tatcaatgac
     1081 ttatctcctg acttcccgcc ttgcttttcc taaacagaac aatcgtagaa tattcttgaa
     1141 gggttagatc gtcactgttt tctgttcgat actgtgacat tcagcacttg attcgctatg
     1201 gagctgacag gaaggtttcg agcgaaaatc tacagttatt cagtcgtttt cttatcggtc
     1261 accattattc ttttagacat tgatcctatg aaactgccgc aaagttgctg gcgggaactg
     1321 aagttgcgta gagaaggggt caataattga gtcaaaaaag gaacgcggcc gtcagattaa
     1381 agccgcggga caaagtccat aatttccaag agttgttgaa tttcccagct tttttgaaag
     1441 cctttgtatc atactaacaa gtaaaaaagg tttaaacacc aaaattaacc tatatttctg
     1501 gttagcttta ccatgacaga tgacttgctc ctggaaacat ttgcgctgcc agtctgtatg
     1561 gctgagttgt gtatttattg ttcgc
//
LOCUS       DAZKHF020000486         1568 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2201S31241
            SAMN43267800-rid25106413.denovo.489, whole genome shotgun sequence.
ACCESSION   DAZKHF020000486 DAZKHF020000000
VERSION     DAZKHF020000486.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267800
            Sequence Read Archive: SRR30307263
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1568)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1568)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 1568)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350581.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350581.2
            Genome Coverage       :: 151x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:21:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,652
            CDSs (total)                      :: 5,557
            Genes (coding)                    :: 5,320
            CDSs (with protein)               :: 5,320
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 237
            CDSs (without protein)            :: 237
            Pseudo Genes (ambiguous residues) :: 0 of 237
            Pseudo Genes (frameshifted)       :: 102 of 237
            Pseudo Genes (incomplete)         :: 130 of 237
            Pseudo Genes (internal stop)      :: 52 of 237
            Pseudo Genes (multiple problems)  :: 41 of 237
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1568
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267800-rid25106413.denovo.489"
                     /serotype="O 26:H11"
                     /isolate="2201S31241"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-01"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            <1..147
                     /locus_tag="ACHOSC_RS25045"
                     /old_locus_tag="pgaptmp_005009"
     CDS             <1..147
                     /locus_tag="ACHOSC_RS25045"
                     /old_locus_tag="pgaptmp_005009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4550757.1"
                     /translation="IIAHRLSTVKNANRIIVMDNGFISEDGTHKELISKKDSLYAYLY
                     QLQA"
     gene            151..>1568
                     /gene="ehxD"
                     /locus_tag="ACHOSC_RS25050"
                     /old_locus_tag="pgaptmp_005010"
     CDS             151..>1568
                     /gene="ehxD"
                     /locus_tag="ACHOSC_RS25050"
                     /old_locus_tag="pgaptmp_005010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enterohemolysin T1SS ABC transporter subunit
                     EhxD"
                     /protein_id="HGU4550758.1"
                     /translation="MRFYMKGLWDLVCRYKTVFSDVWKIRHTLDAPVREKDEYAFLPA
                     HLELIETPVSRRSHFVVWSILLFVIISLLLSVLGKVEVVSVANGKFTHSGRSKEIKPI
                     ENAIVEKIMVKDGSFVKKNDPLVELTVPGVESDILKSEASLLYEKTEQYRYAILSESI
                     QRNELPEIRITDFPGGEDNAGGEHFQRVSSLIKEQFMTWQNRKNQKQLTLNKKIVERD
                     AALARVSLYEHQVSQEGRKLNDFKYLLNKKAVSQHSVMEQENSYIQAKNEHAVWLAQV
                     SQLEKEIELVREELALETNIFRSEIIEKHRKSTDNIVLLEHELEKNRQRKASSFIKAP
                     VSGTVQELNIHTEGGVVTTAETLMIIVPDNDILEVTASVLNKDIGFIQPGQEVVIKVD
                     AYPYTRHGYLTGKVKNITADSVSVPDTGLVFNVIISVDRNDIQGERKKIPVTAGMTVM
                     AEIKTGVRSVISYLLSPLKETI"
BASE COUNT          545 a          236 c          332 g          455 t
ORIGIN      
        1 attattgcac acaggttgtc aactgtaaaa aatgcaaaca gaataattgt tatggataat
       61 ggctttattt ctgaagatgg cacacataaa gagcttatct ccaaaaaaga cagtttatat
      121 gcatatttat atcagttgca ggcataacgt atgcgctttt acatgaaagg tttatgggat
      181 ttggtttgcc gttataagac ggttttttct gatgtctgga aaattcgtca cacacttgat
      241 gctcctgtga gagaaaaaga tgagtatgca tttcttcctg ctcaccttga actcattgaa
      301 acaccagtat ccagacgttc tcattttgtt gtatggagta ttttattatt tgtaattata
      361 tctcttctct tatctgttct tgggaaagtt gaagtggttt cagtagcaaa tggtaagttt
      421 actcatagtg gaagaagcaa agaaataaaa ccgatagaga atgcgattgt agaaaaaata
      481 atggtaaaag atggctcttt tgtaaaaaaa aatgatccat tagttgaatt gacggtgcct
      541 ggtgttgaat ctgatatttt aaaatcagaa gcatctttgt tgtatgaaaa aacagaacaa
      601 tatcgctacg caattctttc tgaatcaata caacggaatg agcttcctga aataaggata
      661 acagattttc ctggcggaga agataacgcg ggaggtgaac attttcagag ggttagctca
      721 ttaataaaag agcaattcat gacatggcag aacagaaaaa atcaaaagca gctcacatta
      781 aataaaaaaa tagttgaacg ggatgcagcg cttgctcgtg ttagcctgta tgagcatcag
      841 gtatcacagg aaggaagaaa actcaatgat tttaagtatt tgttgaataa aaaagctgtt
      901 tctcaacatt cagttatgga gcaggaaaat agctatattc aggcaaaaaa tgaacatgca
      961 gtctggcttg cacaggtttc tcaacttgaa aaagaaatag aacttgtgcg ggaagaactg
     1021 gcactggaga cgaatatctt cagaagtgaa attatcgaga agcacagaaa atcaacagat
     1081 aacattgtgt tgctggagca tgagcttgaa aaaaacaggc agagaaaagc atcatctttt
     1141 attaaagctc ctgtgagtgg tactgttcag gagttaaata tacatacaga aggtggtgtt
     1201 gtcacaacag cagaaacgct gatgattatt gtccctgata atgatattct cgaagtaaca
     1261 gcatctgtac ttaacaagga tatcggtttt atacaacctg gacaggaggt cgttattaaa
     1321 gtagatgcat atccatatac acgtcatggt taccttacag ggaaagtaaa aaatataact
     1381 gcagattctg tttctgttcc ggatacaggg cttgtattta acgtgattat atctgttgat
     1441 cggaatgata tacagggaga aagaaaaaaa atccctgtta cagctggaat gactgttatg
     1501 gcagaaataa aaaccggggt gcgtagtgtt atcagctatc ttcttagtcc attaaaggaa
     1561 actattaa
//
LOCUS       DAZKHF020000487         1551 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2201S31241
            SAMN43267800-rid25106413.denovo.490, whole genome shotgun sequence.
ACCESSION   DAZKHF020000487 DAZKHF020000000
VERSION     DAZKHF020000487.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267800
            Sequence Read Archive: SRR30307263
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1551)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1551)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 1551)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350581.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350581.2
            Genome Coverage       :: 151x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:21:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,652
            CDSs (total)                      :: 5,557
            Genes (coding)                    :: 5,320
            CDSs (with protein)               :: 5,320
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 237
            CDSs (without protein)            :: 237
            Pseudo Genes (ambiguous residues) :: 0 of 237
            Pseudo Genes (frameshifted)       :: 102 of 237
            Pseudo Genes (incomplete)         :: 130 of 237
            Pseudo Genes (internal stop)      :: 52 of 237
            Pseudo Genes (multiple problems)  :: 41 of 237
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1551
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267800-rid25106413.denovo.490"
                     /serotype="O 26:H11"
                     /isolate="2201S31241"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-01"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            98..1495
                     /gene="ccp"
                     /locus_tag="ACHOSC_RS25055"
                     /old_locus_tag="pgaptmp_005011"
     CDS             98..1495
                     /gene="ccp"
                     /locus_tag="ACHOSC_RS25055"
                     /old_locus_tag="pgaptmp_005011"
                     /EC_number="1.11.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c peroxidase"
                     /protein_id="HGU4550759.1"
                     /translation="MKMVSRITAIGLAGVAICYLGLSGYVWYHDNKRSKQADVQASAV
                     SENNKVLGFLREKGCDYCHTPSAELPAYYYIPGAKQLMDYDIKLGYKSFNLEAVRAAL
                     LADKPVSQSDLNKIEWVMQYETMPPTRYTALHWAGKVSDEERAEILAWIAKQRAEYYA
                     SNDTAPEHRNEPVQPIPQKLPTDAQKVALGFALYHDPRLSADSTISCAHCHALNAGGV
                     DGRKTSIGVGGAVGPINAPTVFNSVFNVEQFWDGRAATLQDQAGGPPLNPIEMASKSW
                     DEIIAKLEKDPQLKAQFLEVYPQGFSGENITDAIAEFEKTLITPDSPFDKWLRGDENA
                     LTAQQKKGYQLFKDNKCATCHGGIILGGRSFEPLGLKKDFNFGEITAADIGRMNVTKE
                     ERDKLRQKVPGLRNVALTAPYFHRGDVPTLDGAVKLMLRYQVGKELPQEDVDDIVAFL
                     HSLNGVYTPYMQDKQ"
BASE COUNT          414 a          321 c          420 g          396 t
ORIGIN      
        1 tccctgtttt ttatgatggc tttgtctcgc agagacgttt gcagcattct ggaaaatttt
       61 ctgggtcaca ataactcacc accgtgaggg aagaataatg aaaatggtct cacgtattac
      121 cgcgatcggc ctggctggcg tcgcgatttg ctatttaggg ttatctggtt atgtgtggta
      181 ccacgataat aaacgcagta aacaggccga tgttcaggca tctgctgtca gtgaaaataa
      241 taaggtttta ggctttctcc gcgaaaaagg atgcgactat tgccacacgc cttcggcaga
      301 attacccgcc tattattata ttcctggcgc gaaacagttg atggattacg acattaagct
      361 tggatataaa tcttttaacc tcgaggccgt gcgtgcggca ctgctggctg ataaacccgt
      421 ttcgcaaagc gatctgaata agattgaatg ggtgatgcag tatgaaacta tgccaccaac
      481 gcgttatacc gcgctacact gggcgggtaa ggtgagtgat gaagagcggg cggaaatact
      541 ggcctggatt gcaaaacagc gcgcggaata ttacgccagc aatgatactg ctccggagca
      601 tcgcaatgaa ccggtgcagc ccatcccgca aaaactgcct accgatgcgc aaaaagtggc
      661 gttgggcttt gcgctgtatc acgatccccg tttatcggct gatagcacca tttcatgcgc
      721 tcattgccat gcgttgaatg cggggggcgt cgatggcaga aaaacatcga ttggtgttgg
      781 tggcgcagtt gggccgatta acgcgccgac ggtatttaac tcagtattta acgttgagca
      841 gttctgggat ggtcgtgcgg caacattgca ggatcaggca ggtggaccgc cgttgaaccc
      901 gattgaaatg gcgtcgaaat cctgggacga aattattgct aagctggaaa aagatccgca
      961 gcttaaagcg cagttcctcg aagtttatcc gcaaggtttc agtggcgaaa atattactga
     1021 tgccattgct gaatttgaga aaacattaat tacgccggat tccccatttg ataaatggtt
     1081 gcgtggagat gaaaatgcgc tgacggcgca acagaaaaaa ggctatcaat tatttaaaga
     1141 taataaatgt gcaacttgtc atggtggtat tattctcggc ggacgttcct ttgaaccgtt
     1201 ggggctgaaa aaagacttta actttgggga aattacggcg gcggatattg gtcgtatgaa
     1261 tgtgactaaa gaagagcgtg ataaattgcg tcagaaagta cccggtttac gtaacgttgc
     1321 tttaacggca ccgtacttcc atcgcggtga cgtgccgacg ctggacgggg cggtgaaact
     1381 gatgctgcgc tatcaggtag gcaaagagct gccgcaggag gatgtggatg atatcgtagc
     1441 tttcctgcac agtctgaacg gggtgtacac gccgtatatg caggataaac aataattaat
     1501 ttgatcgccc gaacaggaat gtttgggcga tttttattac gataataaag t
//
LOCUS       DAZKHF020000488         1546 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2201S31241
            SAMN43267800-rid25106413.denovo.491, whole genome shotgun sequence.
ACCESSION   DAZKHF020000488 DAZKHF020000000
VERSION     DAZKHF020000488.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267800
            Sequence Read Archive: SRR30307263
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1546)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1546)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 1546)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350581.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350581.2
            Genome Coverage       :: 151x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:21:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,652
            CDSs (total)                      :: 5,557
            Genes (coding)                    :: 5,320
            CDSs (with protein)               :: 5,320
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 237
            CDSs (without protein)            :: 237
            Pseudo Genes (ambiguous residues) :: 0 of 237
            Pseudo Genes (frameshifted)       :: 102 of 237
            Pseudo Genes (incomplete)         :: 130 of 237
            Pseudo Genes (internal stop)      :: 52 of 237
            Pseudo Genes (multiple problems)  :: 41 of 237
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1546
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267800-rid25106413.denovo.491"
                     /serotype="O 26:H11"
                     /isolate="2201S31241"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-01"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            complement(<1..356)
                     /locus_tag="ACHOSC_RS25060"
                     /old_locus_tag="pgaptmp_005012"
     CDS             complement(<1..356)
                     /locus_tag="ACHOSC_RS25060"
                     /old_locus_tag="pgaptmp_005012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HGU4550760.1"
                     /translation="MNNVIPLQNSPERVSLLPIAPGVDFATALSLRRMATSTGATPAY
                     LLAPEVSALLFYMPDQRHHMLFATLWNTGMRIGEARMLTPESFDLDGVRPFVRILSEK
                     VRARRGRPPKDEVRLVP"
     gene            484..1344
                     /locus_tag="ACHOSC_RS25065"
                     /old_locus_tag="pgaptmp_005013"
     CDS             484..1344
                     /locus_tag="ACHOSC_RS25065"
                     /old_locus_tag="pgaptmp_005013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HGU4550761.1"
                     /translation="MKITDHKLSEGIALTFRVPEGNMKHPLIILCHGFCGIRNVLLPC
                     FANAFTEAGFATITFDYRGFGESEGERGRLVPAMQIEDIISVINWAEKQACIDNQRIG
                     LWGTSLGGCHVFSAAAQDQRVKCIVSQLAFADGDALITGEMNELERASFLSTLNKMAE
                     KKKNTGKEMFVGVTRVLNDDESKVFFEKVKAQHPEMDIKIPFLTVMETLQYKPAESAA
                     RVQCPVLVVIAGQDSVNPPEQGRALYDAVASGTKELYEEADACHYDIYEGTFFERVAA
                     VQTQWFKKYI"
BASE COUNT          420 a          303 c          406 g          417 t
ORIGIN      
        1 ggaaccagac ggacttcatc ttttggcggg cgtccgcgtc gcgcgcgcac tttttcggac
       61 aggatccgca caaacggtct tacaccatcc agatcaaatg attccggtgt cagcatccgg
      121 gcttcgccaa tacgcattcc ggtattccag agggtggcga acagcatatg gtgacgctga
      181 tccggcatat agaaaagaag ggcactcact tccggggcca gcaggtaggc cggtgtggcc
      241 cctgtggaag tggccattct tctcagggag agcgctgttg caaaatccac ccccggcgca
      301 atgggtaaca gggagacgcg ttctggtgaa ttctgcaggg gaatgacatt gttcatgagt
      361 tatttttcac gtagcacacg cactgtttat ggatactgat tgcgctgggc attctggtat
      421 tgtgactggt atgatgctgg cgctgattct gatgattaat aaacaacata acacaggata
      481 tttatgaaaa ttacggatca caaactttct gaaggtattg ctctgacatt tcgtgttcct
      541 gaaggaaata tgaaacatcc tttgattatc ttatgccatg gattttgcgg tatccggaat
      601 gttctgctcc cttgctttgc gaatgctttc acagaagcag ggttcgcaac gattactttt
      661 gattatcgtg gattcggtga gagtgaagga gaacggggcc gtctggttcc agcaatgcag
      721 atcgaagata ttatttcagt tatcaattgg gcagaaaagc aggcatgtat tgataatcag
      781 cgtatcgggt tatggggaac atcgctgggt ggttgtcatg tgttcagtgc agctgcgcaa
      841 gatcaaagag tgaaatgtat tgtcagccag ctggcttttg ctgatggtga tgctctgatt
      901 accggagaga tgaatgaatt ggagagagct tcttttctgt ccacactgaa taaaatggca
      961 gagaaaaaga aaaacacagg gaaggagatg tttgtggggg ttacacgggt actaaatgat
     1021 gatgagtcga aggtattctt cgagaaagtc aaagctcagc atcccgaaat ggatataaaa
     1081 atacctttcc ttactgtcat ggaaacgttg caatataaac cggccgagag cgcagccaga
     1141 gtccagtgtc cagtattggt tgttattgct ggccaggata gtgttaatcc accggaacag
     1201 ggaagagcat tatatgatgc tgttgcttct ggtactaaag aattatatga ggaagctgac
     1261 gcatgccatt atgatattta tgagggcaca ttttttgagc gggtagcagc tgtgcaaaca
     1321 caatggttta aaaaatatat ttaatagtga gcctgtgttt accattctgt gtaaatactt
     1381 tttcttaaaa gtgaccgtca aggcggtcac taaactttat aataaagagg catcgttcat
     1441 ttccgggagg cagactggat tgccagcccc ccacttttta ccaagctgca ggtatggggg
     1501 acacggacac tggtgttcac agaaaaaaat aaccatctct gaaatg
//
LOCUS       DAZKHF020000489         1538 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2201S31241
            SAMN43267800-rid25106413.denovo.492, whole genome shotgun sequence.
ACCESSION   DAZKHF020000489 DAZKHF020000000
VERSION     DAZKHF020000489.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267800
            Sequence Read Archive: SRR30307263
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1538)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1538)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 1538)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350581.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350581.2
            Genome Coverage       :: 151x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:21:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,652
            CDSs (total)                      :: 5,557
            Genes (coding)                    :: 5,320
            CDSs (with protein)               :: 5,320
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 237
            CDSs (without protein)            :: 237
            Pseudo Genes (ambiguous residues) :: 0 of 237
            Pseudo Genes (frameshifted)       :: 102 of 237
            Pseudo Genes (incomplete)         :: 130 of 237
            Pseudo Genes (internal stop)      :: 52 of 237
            Pseudo Genes (multiple problems)  :: 41 of 237
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1538
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267800-rid25106413.denovo.492"
                     /serotype="O 26:H11"
                     /isolate="2201S31241"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-01"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            <1..345
                     /locus_tag="ACHOSC_RS25070"
                     /old_locus_tag="pgaptmp_005014"
     CDS             <1..345
                     /locus_tag="ACHOSC_RS25070"
                     /old_locus_tag="pgaptmp_005014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter small permease subunit"
                     /protein_id="HGU4550762.1"
                     /translation="LFVWLTFIGAIVAFMDNAHVQVTFLVEKLSPAWQRRVALVTHSL
                     ILFICGALAWGATLKTIQDWSDYSPILGLPIGLMYAACLPTSLVIAFFELRHLYQLIT
                     RSNSLTSPPQGA"
     gene            348..>1538
                     /gene="yiaN"
                     /locus_tag="ACHOSC_RS25075"
                     /old_locus_tag="pgaptmp_005015"
     CDS             348..>1538
                     /gene="yiaN"
                     /locus_tag="ACHOSC_RS25075"
                     /old_locus_tag="pgaptmp_005015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031317 - tripartite ATP-independent
                     periplasmic transporter complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-diketo-L-gulonate TRAP transporter large
                     permease YiaN"
                     /protein_id="HGU4550763.1"
                     /translation="MAVLIFLGCLLGGIAIGLPIAWALLLCGAALMFWLDMFDVQIMA
                     QTLVNGADSFSLLAIPFFVLAGEIMNAGGLSKRIVDLPMKLVGHKPGGLGYVGVLAAM
                     IMASLSGSAVADTAAVAALLVPMMRSANYPVNRAAGLIASGGIIAPIIPPSIPFIIFG
                     VSSGLSISKLFMAGIAPGMMMGATLMLTWWWQASRLNLPRQQKATMQEIWHSFVSGIW
                     ALFLPVIIIGGFRSGLFTPTEAGAVAAFYALFVATVIYREMTFATLWHVLIGAAKTTS
                     VVMFLVASAQVSAWLITIAELPMMVSDLLQPLVDSPRLLFIVIMVAILIVGMVMDLTP
                     TVLILTPVLMPLVKEAGIDPIYFGVMFIINCSIGLITPPIGNVLNVISGVAKLKFDDA
                     VRGVF"
BASE COUNT          285 a          392 c          400 g          461 t
ORIGIN      
        1 ttatttgtct ggttaacatt tattggcgcg attgtggctt ttatggataa cgcccacgtt
       61 caggtcacct ttctggtgga gaaactctcc cccgcatggc aacgacgagt cgctctggtt
      121 acccattcct taatcttatt tatttgtggc gcactggcct ggggcgcaac gctaaaaaca
      181 attcaggact ggagcgatta ttcaccgatc ctcggtttac ccatcggcct gatgtatgcc
      241 gcttgtctgc ccaccagcct cgttatcgca ttctttgaac tgcgtcattt atatcaactc
      301 atcacgcgta gcaattctct aacgtcgcca ccgcaaggag cttaatcatg gctgtgctga
      361 tttttctggg ctgtctgttg ggtggtattg ctatcggttt gcctattgcc tgggcactgt
      421 tgttgtgcgg ggcggcgtta atgttctggc tggacatgtt tgatgtccag atcatggcac
      481 aaacgctggt gaacggagcc gatagcttct ccctgctggc gattccgttc tttgtgctgg
      541 cgggtgaaat catgaatgcg ggcggcttgt caaaacgcat tgttgacctg ccgatgaaac
      601 tggtggggca taaaccaggc gggctgggct acgtcggcgt gctggcagct atgattatgg
      661 cgagcctttc tggttctgcc gttgcggata ccgccgccgt tgccgctcta ctggtgccga
      721 tgatgcgcag cgccaactac ccggtcaacc gggcggcggg gctgattgcc tctggcggca
      781 ttatcgcgcc aattattccc ccttccattc cgtttattat cttcggcgtt tccagcggat
      841 tatccatcag taagctgttt atggcgggca ttgcccctgg catgatgatg ggcgcaacgc
      901 tgatgcttac ctggtggtgg caggccagtc gccttaattt accccgccag caaaaagcaa
      961 cgatgcagga aatctggcac tcttttgtct ccggtatctg ggcgctgttt cttccagtca
     1021 tcattattgg cgggtttcgc tccggtctgt ttacccctac cgaagccggg gccgttgctg
     1081 ctttctatgc gctgtttgtc gccacagtta tttaccgtga aatgaccttc gccacacttt
     1141 ggcatgtact cattggcgcg gcgaaaacca cctcagtggt tatgtttctg gtggcctcgg
     1201 cacaagtttc cgcctggctg attaccattg ctgaactgcc gatgatggtt tctgatttac
     1261 tgcaaccgtt ggtcgattca ccgcgtctgc tgtttatcgt cattatggtg gctatcctga
     1321 ttgtcggcat ggtcatggat ttaacgccga cggtattaat tcttaccccg gtattaatgc
     1381 ctttagttaa agaggcagga atcgatccga tttatttcgg tgtcatgttt atcattaact
     1441 gctcaatcgg cttaattaca ccgcctatcg gtaatgtact taacgttatt tcaggggtgg
     1501 caaaactcaa attcgatgat gcagtcagag gcgttttc
//
LOCUS       DAZKHF020000490         1537 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2201S31241
            SAMN43267800-rid25106413.denovo.493, whole genome shotgun sequence.
ACCESSION   DAZKHF020000490 DAZKHF020000000
VERSION     DAZKHF020000490.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267800
            Sequence Read Archive: SRR30307263
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1537)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1537)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 1537)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350581.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350581.2
            Genome Coverage       :: 151x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:21:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,652
            CDSs (total)                      :: 5,557
            Genes (coding)                    :: 5,320
            CDSs (with protein)               :: 5,320
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 237
            CDSs (without protein)            :: 237
            Pseudo Genes (ambiguous residues) :: 0 of 237
            Pseudo Genes (frameshifted)       :: 102 of 237
            Pseudo Genes (incomplete)         :: 130 of 237
            Pseudo Genes (internal stop)      :: 52 of 237
            Pseudo Genes (multiple problems)  :: 41 of 237
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1537
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267800-rid25106413.denovo.493"
                     /serotype="O 26:H11"
                     /isolate="2201S31241"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-01"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            <1..442
                     /locus_tag="ACHOSC_RS25080"
                     /old_locus_tag="pgaptmp_005016"
     CDS             <1..442
                     /locus_tag="ACHOSC_RS25080"
                     /old_locus_tag="pgaptmp_005016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="terminase small subunit"
                     /protein_id="HGU4550764.1"
                     /translation="LPGAPVSNKRGQLDLDAWRDFYISYLRRSKNDVPDGDSEEDYEE
                     KLLIARWELTAEQAVTQQLKNEVSKGKLIDTGFCIFALSKLAMALSSTLDSIPLSMQR
                     QFPDLTPRHLDHLKTLIAKGANQCARAGDKLPDLLDEYIRATTE"
     gene            414..>1537
                     /locus_tag="ACHOSC_RS25085"
                     /old_locus_tag="pgaptmp_005017"
     CDS             414..>1537
                     /locus_tag="ACHOSC_RS25085"
                     /old_locus_tag="pgaptmp_005017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="terminase gpA endonuclease subunit"
                     /protein_id="HGU4550765.1"
                     /translation="MNISEQQLNNMMSAVTTALQPLIRALPVTPVEWADQNYYLPKES
                     SYGEGEWKTLPFQIAIMNSMGNDQIRTVNLIKSARVGYTKMLLGVVGYFIEHKSRNSL
                     LFQPTDSAAEDFMKSHVEATIRNVPCLKDLSPWLGRKHRDNTLTLKRFSSGVGFWCLG
                     GAAAKNYREKSVDVVCYDELSSFEPDVEKEGSPTLLGDKRIEGSVWPKSIRGSTPKIK
                     GTCQIEKAANESAHFMRFYVPCPHCGEEQYLKFGDESTPFGLKWEKDSPESVFYLCEH
                     HGCVIHQSELDQSNGRWICENTGMWTRDGLTFFSARGDEIPPPRSITFHIWTAYSPFT
                     TWVQIVYDWLDALKDPNGLKTFVNTTLGETWEEAVGEKLD"
BASE COUNT          394 a          361 c          415 g          367 t
ORIGIN      
        1 attaccgggt gccccggtct cgaataagcg agggcaactg gatctggatg cctggcgcga
       61 tttttacata tcgtatctga gaagaagtaa aaacgatgtg cctgatggcg atagcgaaga
      121 agactatgaa gagaaattgc ttattgccag atgggaactg acagcagaac aggctgttac
      181 acagcagtta aaaaatgagg tgtcaaaagg aaaactgatt gacaccgggt tctgtatttt
      241 tgccctcagt aagctggcaa tggcgttatc cagtacgctt gattccatcc ctttatccat
      301 gcagcgacag tttcctgatt taacaccgcg ccatcttgac catctgaaaa cccttattgc
      361 taagggggca aatcagtgtg cgcgggcagg ggataaatta ccggatttac ttgatgaata
      421 tatcagagca acaactgaat aatatgatga gcgctgtcac aactgcatta cagcccctga
      481 taagggcatt gccggtgacg ccagttgaat gggctgatca aaattattat ctgcctaaag
      541 aatcttcata tggtgagggc gaatggaaaa cgctgccatt ccagatcgcc atcatgaaca
      601 gcatggggaa tgatcagatc cgcactgtta atctgattaa atctgcccgt gttggctata
      661 caaagatgtt gctgggggtg gtcgggtatt ttattgagca taaatcccga aacagtctgc
      721 tttttcagcc cacggattct gccgctgaag attttatgaa gtctcacgtg gaggcgacga
      781 ttcggaacgt gccatgcctg aaagaccttt ccccatggct gggtcgtaaa catcgtgaca
      841 atactctcac gctgaaacgc ttttcatcgg gcgtcggttt ctggtgcctg ggcggcgctg
      901 ccgccaaaaa ctaccgtgaa aaatccgtgg acgtggtctg ctatgacgaa ctttcctcgt
      961 tcgagccgga tgtcgaaaaa gagggctcgc caaccctgct gggggataag cgtattgagg
     1021 ggtcggtgtg gccaaaatcc attcgcggct cgacgcctaa aatcaaaggc acctgccaga
     1081 tcgaaaaagc cgctaacgag tcggcgcatt ttatgcgttt ttatgtgccc tgcccgcact
     1141 gtggggagga gcagtatctg aaatttggcg atgagtccac gccttttggg cttaaatggg
     1201 agaaggacag ccctgaaagt gttttctacc tctgtgaaca tcatggctgc gtgatccatc
     1261 agtctgaact ggaccagagc aacgggcggt ggatctgtga aaacacgggc atgtggaccc
     1321 gtgacggtct gacgtttttc agcgcccggg gtgatgaaat tccgccgccg cgctccatca
     1381 cgttccatat ctggacggcg tacagtccgt tcaccacctg ggtacagatt gtctatgact
     1441 ggctggatgc actgaaagat cccaacggcc tgaaaacctt tgtgaacacc acgctgggcg
     1501 agacctggga agaggccgtg ggcgaaaaac tcgatca
//
LOCUS       DAZKHF020000491         1531 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2201S31241
            SAMN43267800-rid25106413.denovo.494, whole genome shotgun sequence.
ACCESSION   DAZKHF020000491 DAZKHF020000000
VERSION     DAZKHF020000491.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267800
            Sequence Read Archive: SRR30307263
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1531)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1531)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 1531)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350581.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350581.2
            Genome Coverage       :: 151x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:21:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,652
            CDSs (total)                      :: 5,557
            Genes (coding)                    :: 5,320
            CDSs (with protein)               :: 5,320
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 237
            CDSs (without protein)            :: 237
            Pseudo Genes (ambiguous residues) :: 0 of 237
            Pseudo Genes (frameshifted)       :: 102 of 237
            Pseudo Genes (incomplete)         :: 130 of 237
            Pseudo Genes (internal stop)      :: 52 of 237
            Pseudo Genes (multiple problems)  :: 41 of 237
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1531
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267800-rid25106413.denovo.494"
                     /serotype="O 26:H11"
                     /isolate="2201S31241"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-01"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            <1..660
                     /locus_tag="ACHOSC_RS25090"
                     /old_locus_tag="pgaptmp_005018"
     CDS             <1..660
                     /locus_tag="ACHOSC_RS25090"
                     /old_locus_tag="pgaptmp_005018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001764249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF968 domain-containing protein"
                     /protein_id="HGU4550766.1"
                     /translation="PGAIRLCWHCDNQLRDQFTERLESMATDNCARWVLSVVRRDLGF
                     DDSHVVTMPELCWWLIRNDLADALPESAARKALRLPKPVVPSVTRESDLVPSVPATSI
                     IQDKAKKVLVLKVDPESPESFMLRPKRRRWVNEKYTRWVKTQPCACCGKPADDPHHLI
                     GHGQCGMGTKAHDLFVLPLCRKHHDELHADTVAFEEKYGSQLELIFRFIDRALAIGVL
                     A"
     gene            674..1426
                     /locus_tag="ACHOSC_RS25095"
                     /old_locus_tag="pgaptmp_005019"
     CDS             674..1426
                     /locus_tag="ACHOSC_RS25095"
                     /old_locus_tag="pgaptmp_005019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416077.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="antitermination protein"
                     /protein_id="HGU4550767.1"
                     /translation="MNLEALPKYYSPKSPKLSDDAPATGSGGLTITDVMAAQGMVQSK
                     ATLGFALFLAKVGVQDPQFAIEGLLNYAMALDNPTLNKLSEETRLQIIPYLVNFAFAD
                     YSRSAASKARCEHCAGTGFHNVLREVVKHSRSGESVIKEEWVKELCQHCHGKGEVSTA
                     CRGCKGKGIVLDEKRTRLHGTPVYKVCGRCNGNRFSRLPTTLARLHVQKLVPDLTDYQ
                     WYKGYADVIDKLVTKCWQEEAYAEIQLRKVTR"
BASE COUNT          389 a          319 c          437 g          386 t
ORIGIN      
        1 ccgggcgcaa tccgtctgtg ctggcactgc gataaccagc tgcgcgatca gttcacggaa
       61 cggctggaat caatggcaac ggataactgt gcccgctggg tgttgtctgt tgtgcgtcgg
      121 gatctcggtt ttgatgacag tcacgttgtg acaatgccgg aactgtgctg gtggctgatt
      181 cgtaatgatc tggcggatgc cttaccggaa agtgcagccc gtaaggcact gagattaccg
      241 aagcctgttg tgccgtctgt cacccgggaa agtgaccttg tgccttcggt tcctgccacc
      301 agcatcatcc aggataaagc gaaaaaggtg ctggtgctga aagtggatcc ggagtcgccg
      361 gagtctttta tgttacgccc aaaacgtcgc cgctgggtta atgaaaagta cacgcgctgg
      421 gttaagacac agccgtgtgc atgttgtgga aagcccgctg atgatcccca ccacctgata
      481 ggccacggtc agtgtggaat gggtacaaaa gcgcatgacc tttttgtgtt gcctttgtgc
      541 agaaagcatc acgacgagct gcatgcggat accgtggcat ttgaagagaa gtatggctct
      601 cagctggagc tgatatttcg ttttatcgat cgtgcgctgg caattggcgt actggcgtaa
      661 gtggagaacg agcatgaacc ttgaagcctt accgaaatat tactccccga aatctccaaa
      721 actgagcgat gacgcaccgg cgacaggctc tggtggttta acaattacgg atgtgatggc
      781 tgcgcagggg atggtgcagt caaaagcaac gcttgggttt gctttattcc tggcaaaagt
      841 tggtgttcag gatcctcagt ttgcgattga aggtctgctc aattacgcga tggcactgga
      901 taacccgaca ttgaataaat tgagtgaaga aacccggcta cagatcattc cttaccttgt
      961 gaattttgcc tttgctgatt attccaggtc tgcggcaagt aaggctcgct gtgagcattg
     1021 tgctggtact ggatttcata atgtattgcg cgaggtggtg aaacactcca gaagcgggga
     1081 atctgttatt aaggaagagt gggtgaagga actatgtcag cattgtcatg gtaagggaga
     1141 agtcagcaca gcatgcagag ggtgcaaggg taaaggtatt gtcctggatg aaaaaagaac
     1201 ccggcttcat ggcacgcctg tttataaggt ttgtgggcgt tgcaatggaa accggtttag
     1261 ccgtttacca accacactgg cgcggcttca tgtccagaag ctggtaccag acctgacgga
     1321 ttatcagtgg tacaaaggat atgcagatgt cattgataaa ctggttacaa agtgctggca
     1381 ggaagaagca tatgcagaga tacaattgag aaaggtgaca agataaatgg ttttcgccga
     1441 agatgacgac atgatgcttg catttttcaa aaaatatgga taagattttc ccaacgatgg
     1501 gctttgtatg tctaccgttg ataagattta a
//
LOCUS       DAZKHF020000492         1528 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2201S31241
            SAMN43267800-rid25106413.denovo.495, whole genome shotgun sequence.
ACCESSION   DAZKHF020000492 DAZKHF020000000
VERSION     DAZKHF020000492.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267800
            Sequence Read Archive: SRR30307263
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1528)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1528)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 1528)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350581.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350581.2
            Genome Coverage       :: 151x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:21:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,652
            CDSs (total)                      :: 5,557
            Genes (coding)                    :: 5,320
            CDSs (with protein)               :: 5,320
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 237
            CDSs (without protein)            :: 237
            Pseudo Genes (ambiguous residues) :: 0 of 237
            Pseudo Genes (frameshifted)       :: 102 of 237
            Pseudo Genes (incomplete)         :: 130 of 237
            Pseudo Genes (internal stop)      :: 52 of 237
            Pseudo Genes (multiple problems)  :: 41 of 237
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1528
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267800-rid25106413.denovo.495"
                     /serotype="O 26:H11"
                     /isolate="2201S31241"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-01"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            <1..80
                     /locus_tag="ACHOSC_RS25100"
                     /old_locus_tag="pgaptmp_005020"
     CDS             <1..80
                     /locus_tag="ACHOSC_RS25100"
                     /old_locus_tag="pgaptmp_005020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4550768.1"
                     /translation="GWFIGSIHDTEDGPVCVWLRNKAEA"
     gene            386..964
                     /locus_tag="ACHOSC_RS25105"
                     /old_locus_tag="pgaptmp_005021"
     CDS             386..964
                     /locus_tag="ACHOSC_RS25105"
                     /old_locus_tag="pgaptmp_005021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001204666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sce7726 family protein"
                     /protein_id="HGU4550769.1"
                     /translation="MRDIDVRKAVHAKILRDHHKDPDTLIIDEFTMNLGASRADIAVI
                     NGLIHGYELKSKSDNLLRLPAQVQHYSSVMDKVTLVVSDCHLYDALSIVPSWWGIKQV
                     TQGARQGIHLKTIRTSKLNPQVDKLSLTMLLWKDELLSLLSDVGELQNLKNKPKRVLW
                     SKLANSMDVGELREAVRVKLKARKEWRVAQQP"
     gene            complement(924..>1528)
                     /locus_tag="ACHOSC_RS25110"
                     /old_locus_tag="pgaptmp_005022"
     CDS             complement(924..>1528)
                     /locus_tag="ACHOSC_RS25110"
                     /old_locus_tag="pgaptmp_005022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001721311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="beta family protein"
                     /protein_id="HGU4550770.1"
                     /translation="LRDELTEDKINSGELYTLAMGLINNLAHLNEYRKVILSGGSFPT
                     DLSDISVGLYSQPRIEWILWQSLMNRKELARNVIYSDYGVQHPDFNRLSTRFPSVSAS
                     VRYSGDNDFWVFRGRVANRFGYEQYGKHSEDILAHREYSGPTFCAGDRDIEYYANEYQ
                     AYKANPSGNYKFGSPEVWRRIGQNHHITKVVEQLATLYGL"
BASE COUNT          472 a          302 c          317 g          437 t
ORIGIN      
        1 atggatggtt tattggttca atacatgaca ccgaagatgg cccggtttgt gtatggctga
       61 gaaataaggc cgaagcataa aggctataaa ccgactaaca actaaatact gaagatttaa
      121 atcagaaacg atttttatta aacccttaac cggagggatt ctgcaccctc agaacatcag
      181 gaggccgtct gaaagggcgg aacagataat gcttacgttg aaacatttta tcgacatacc
      241 aacatggtta gccgtcattg cttttgttaa aatacacatc cacttttctg tgcaatgttt
      301 aaccactggt catatcaaat ggcattcatg cgaaccatga tatagaatca tggcttgaga
      361 gagtcgatga aagcgcaact atggtatgag agacattgat gtaagaaagg ctgtgcatgc
      421 caagattctg agagatcatc ataaagatcc tgacacccta atcattgatg agtttacgat
      481 gaatctaggg gctagcagag ctgatatagc agtgatcaat gggcttatac atggttatga
      541 gttgaagagc aagagtgata acttgctcag attaccagcg caggtgcaac attactcatc
      601 agtgatggat aaagtaactt tggttgtctc tgattgccat ctttatgatg ctttaagcat
      661 agttccatca tggtggggga taaagcaagt tacgcaaggt gcacggcaag gtatccattt
      721 aaaaacaatt cgaactagca agttgaatcc acaagtggac aaactttcct taacaatgct
      781 tctttggaaa gatgaattgc tttccctatt aagtgatgta ggggagctac agaatttgaa
      841 aaataaacct aaacgcgtct tatggtcaaa actcgccaat agtatggatg ttggcgagct
      901 tcgtgaagct gttcgagtta aacttaaagc ccgtaaagag tggcgagttg ctcaacaacc
      961 ttagttatgt gatggttttg cccaatccta cgccatacct ctgggctacc aaatttatag
     1021 ttaccagagg gattggcttt gtaggcttga tactcgtttg cataatattc tatgtctcta
     1081 tctcccgcac agaatgtagg ccctgaatat tctcgatgag caagaatatc ctcactatgt
     1141 ttaccatatt gttcataacc aaagcgatta gctactcttc ctcgaaatac ccaaaagtca
     1201 ttatctccag agtatctgac gctggcagat acgctaggga atcgcgtcga aagcctatta
     1261 aagtcggggt gctgtactcc ataatcacta taaatcacat ttctggcaag ttcttttcta
     1321 ttcattaaac tctgccataa aatccactcg attcgaggtt gagaatatag accaacagaa
     1381 atatcactga gatctgtagg aaatgaaccc ccagaaagaa tcacttttct gtattcattg
     1441 aggtgcgcca gattgtttat caatcccatt gccaaagtat atagttcgcc ggaattaatt
     1501 ttatcctcgg ttaactcatc tcttaagt
//
LOCUS       DAZKHF020000493         1526 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2201S31241
            SAMN43267800-rid25106413.denovo.496, whole genome shotgun sequence.
ACCESSION   DAZKHF020000493 DAZKHF020000000
VERSION     DAZKHF020000493.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267800
            Sequence Read Archive: SRR30307263
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1526)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1526)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 1526)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350581.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350581.2
            Genome Coverage       :: 151x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:21:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,652
            CDSs (total)                      :: 5,557
            Genes (coding)                    :: 5,320
            CDSs (with protein)               :: 5,320
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 237
            CDSs (without protein)            :: 237
            Pseudo Genes (ambiguous residues) :: 0 of 237
            Pseudo Genes (frameshifted)       :: 102 of 237
            Pseudo Genes (incomplete)         :: 130 of 237
            Pseudo Genes (internal stop)      :: 52 of 237
            Pseudo Genes (multiple problems)  :: 41 of 237
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1526
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267800-rid25106413.denovo.496"
                     /serotype="O 26:H11"
                     /isolate="2201S31241"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-01"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            complement(<1..665)
                     /locus_tag="ACHOSC_RS25115"
                     /old_locus_tag="pgaptmp_005023"
     CDS             complement(<1..665)
                     /locus_tag="ACHOSC_RS25115"
                     /old_locus_tag="pgaptmp_005023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aconitase family protein"
                     /protein_id="HGU4550771.1"
                     /translation="MSSTLREASKDTLQAKDKTYHYYSLPLAAKSLGDITRLPKSLKV
                     LLENLLRWQDGNSVTEEDIHALAGWLKNAHADREIAYRPARVLMQDFTGVPAVVDLAA
                     MREAVKRLGGDTAKVNPLSPVDLVIDHSVTVDRFGDDEAFEENVRLEMERNHERYVFL
                     KWGKQAFSRFSVVPPGTGICHQVNLEYLGKAVWSELQDGEWIAYPDTLVGTDSHTTMI
                     NGLG"
     gene            complement(829..924)
                     /gene="ymiC"
                     /locus_tag="ACHOSC_RS25120"
                     /old_locus_tag="pgaptmp_005024"
     CDS             complement(829..924)
                     /gene="ymiC"
                     /locus_tag="ACHOSC_RS25120"
                     /old_locus_tag="pgaptmp_005024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="small membrane protein YmiC"
                     /protein_id="HGU4550772.1"
                     /translation="MINTNMKYWSWMGAFSLSMLFWAELLWIITH"
     gene            complement(1038..1205)
                     /gene="yciX"
                     /locus_tag="ACHOSC_RS25125"
                     /old_locus_tag="pgaptmp_005025"
     CDS             complement(1038..1205)
                     /gene="yciX"
                     /locus_tag="ACHOSC_RS25125"
                     /old_locus_tag="pgaptmp_005025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588450.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YciX"
                     /protein_id="HGU4550773.1"
                     /translation="MVGQEQLESSPLCQHSDNETETKRECSVVIPDDWQLTSQQQAFI
                     ELFAEDDQPKQ"
     gene            complement(1208..1348)
                     /locus_tag="ACHOSC_RS25130"
                     /old_locus_tag="pgaptmp_005026"
     CDS             complement(1208..1348)
                     /locus_tag="ACHOSC_RS25130"
                     /old_locus_tag="pgaptmp_005026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588449.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YmiA family putative membrane protein"
                     /protein_id="HGU4550774.1"
                     /translation="MRLAMPSGNQEPRRDPELKRKAWLAVFLGSALFWVVVALLIWKV
                     WG"
BASE COUNT          411 a          367 c          355 g          393 t
ORIGIN      
        1 ccaaggccgt tgatcatagt ggtgtgcgag tcagtaccaa cgagtgtatc cggataagca
       61 atccattcac cgtcctgcaa ttcactccac actgctttgc cgagatattc gaggttaacc
      121 tgatggcaaa tgcctgtgcc tggcggcacg acgctaaacc gactgaacgc ttgctttccc
      181 catttcagga acacataacg ttcgtggttg cgctccattt ccaggcgtac gttttcttca
      241 aatgcctcat catcaccaaa acgatcgacg gtcaccgagt ggtcaatgac caggtcgacc
      301 ggtgagagcg ggttaacctt tgcagtatcg ccgccgaggc gtttaaccgc ttcgcgcatt
      361 gccgccagat caacaacggc aggtacgccg gtaaagtcct gcatcagcac ccttgccggg
      421 cggtaggcaa tttcacggtc agcatgggca tttttcagcc atcctgccag cgcgtggata
      481 tcctcttcgg taaccgagtt accatcctgc cagcgcagca ggttttcgag caaaactttg
      541 agtgacttgg gtagacgggt gatatcgccc agtgatttag cagcaagcgg caggctgtag
      601 tagtggtaag ttttatcttt ggcctgcaac gtgtccttac tggcttctcg tagggttgac
      661 gacatagctc ctccttaatg acagggttgc gaagccctga ttctcttcag ggtgaatatt
      721 aaagataaca caaacatcgc gtaacgattt gataacaacc caaattgata aaagaggaga
      781 aaccttgtgg caaagcaaat gacaaccccg ccgcagcggg gtcaaggatc agtgagtaat
      841 gatccagagg agttcggccc agaagagcat cgacagagaa aacgcgccca tccaggacca
      901 atatttcata tttgtgttga tcataggcta cacccttaac ggttattatt ttgtgttgag
      961 ataactcaag ctgttattgc tgcggtgtat taaaagccct ttaattcatt aaaaatgggt
     1021 aataagaatg atgataatta ttgtttcggc tgatcatctt cagcaaacag ttctataaag
     1081 gcttgctgct gcgatgttag ttgccagtcg tctggaataa cgacggaaca ttcccgtttc
     1141 gtttctgttt cattgtcact atgctggcat aatggtgacg actccagttg ctcctgaccg
     1201 accatagtta tccccacact ttccaaatca gcagtgcgac aaccacccag aaaagtgcag
     1261 aaccaagaaa aaccgccagc caggctttac gttttaattc aggatcgcgg cggggttcct
     1321 gatttccaga aggcattgct aacctcatat aatcgccatc acttatcagc aagacgttga
     1381 acgattggtg taattaatca tacgatgaga caaatcctaa agaaacttta gtcaaaaatc
     1441 cagcgaattt acgaatcgtc tccagtgaat aattcaatct tttgacctga aatagatatt
     1501 tgaaaactgg agtttgcgag gcgggt
//
LOCUS       DAZKHF020000494         1507 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2201S31241
            SAMN43267800-rid25106413.denovo.497, whole genome shotgun sequence.
ACCESSION   DAZKHF020000494 DAZKHF020000000
VERSION     DAZKHF020000494.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267800
            Sequence Read Archive: SRR30307263
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1507)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1507)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 1507)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350581.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350581.2
            Genome Coverage       :: 151x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:21:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,652
            CDSs (total)                      :: 5,557
            Genes (coding)                    :: 5,320
            CDSs (with protein)               :: 5,320
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 237
            CDSs (without protein)            :: 237
            Pseudo Genes (ambiguous residues) :: 0 of 237
            Pseudo Genes (frameshifted)       :: 102 of 237
            Pseudo Genes (incomplete)         :: 130 of 237
            Pseudo Genes (internal stop)      :: 52 of 237
            Pseudo Genes (multiple problems)  :: 41 of 237
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1507
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267800-rid25106413.denovo.497"
                     /serotype="O 26:H11"
                     /isolate="2201S31241"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-01"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            complement(<1..578)
                     /locus_tag="ACHOSC_RS25135"
                     /old_locus_tag="pgaptmp_005027"
     CDS             complement(<1..578)
                     /locus_tag="ACHOSC_RS25135"
                     /old_locus_tag="pgaptmp_005027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000633896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha amylase N-terminal ig-like
                     domain-containing protein"
                     /protein_id="HGU4550775.1"
                     /translation="MITVSSLKHSAKSEDSYAYDNETLHVRLRTLRGEVDKVILWIGD
                     PYNWAEGGLDGGNMAGTEAFGWIGGNEIEMEQEAVTEFHDHWFAVFKPQKRRCRYGFI
                     LFGKEGEKFLFGEKRCVDISSPDCEERELSRLNNFFCFPYLNKIDVLNTPSWVKNTVW
                     YQIFPDRFCNGRPEISPEGVEPWGSAPTSFNF"
     gene            complement(598..1431)
                     /locus_tag="ACHOSC_RS25140"
                     /old_locus_tag="pgaptmp_005028"
     CDS             complement(598..1431)
                     /locus_tag="ACHOSC_RS25140"
                     /old_locus_tag="pgaptmp_005028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001583591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HGU4550776.1"
                     /translation="MDKFLRKISTLLVYLFLICNSILVLGPVIWTIMASFKKGNNLFS
                     STFSGIEFTFDHYITLFTDTPYMQWYLNTFILATANMLISLFVVTMTAYVFSRYRFRG
                     KRNTLMSILVLQMFPAFLSMTAIYILLSKANLIDTYTGLLLVYVTGSLPFMTWLVKGY
                     FDAIPTSLDEAAKIDGAGHLTIFIQIILPLAKPILVFVGLVLFTGPWMDFILPTLVLR
                     SEDKMTLAIGIFSWIASNSAENYTLFAAGALLVAVPITLLFVFTQKHITTGLVSGAVK
                     E"
BASE COUNT          499 a          333 c          263 g          412 t
ORIGIN      
        1 ataaagttga atgaagtcgg tgcactgccc cacggttcta ccccttctgg ggatatttca
       61 ggtctgccgt tacagaaacg gtctggaaaa atttggtacc ataccgtatt ttttacccag
      121 gagggggtat ttagaacgtc aattttatta agataaggga agcaaaaaaa gttatttaag
      181 cggcttaact ctcgttcttc gcagtccggt gaactgatat caacacacct tttctcacca
      241 aaaagaaact tctctccctc tttcccaaaa agtataaaac cgtaacggca acggcgtttt
      301 tgaggtttga acactgcaaa ccagtgatca tgaaattcgg tgacagcttc ttgttccatc
      361 tcgatctcgt tgccgccaat ccagccgaac gcttctgtcc cggccatgtt accgccgtca
      421 agaccacctt cagcccaatt atatggatcg cctatccaca gaataacttt atcaacttct
      481 cctcttaatg tgcgtaaccg tacatgcaaa gtttcgttat cataggcata gctatcttca
      541 ctttttgctg agtgttttaa cgaactaact gtaatcataa atactaatcc ctaaaaatta
      601 ttccttaacg gcaccgctaa caagtccagt agttatatgc ttctgtgtga atacaaataa
      661 caatgtgata ggtactgcaa ctaaaagcgc cccagcagca aaaagcgtat aattctctgc
      721 tgaattagaa gcaatccaac taaaaattcc aatcgccagg gtcattttat cctcactacg
      781 aagcaccaac gttggcaaga tgaagtccat ccatggtcca gtgaacaaaa ctaatccaac
      841 gaaaacgaga atcggcttag ccaatggcaa tattatctga ataaaaatag tcagatgccc
      901 tgctccatct attttagcgg cctcatccaa agaagttgga atagcatcaa aataaccttt
      961 taccaaccac gtcatgaagg gcaatgaacc ggttacataa accagaagca acccagtata
     1021 tgtgtctatc aaattggcct ttgatagcag gatataaatt gcagtcattg ataaaaatgc
     1081 aggaaacatt tgcaatacaa gaatactcat taaagtatta cgctttccac gaaatcgata
     1141 ccttgaaaaa acgtaggcgg tcatcgtcac aacgaacagt gaaattaaca tgttagcggt
     1201 agcaagaatg aaagtgttca aataccactg catatatggc gtatccgtga acaacgttat
     1261 gtagtggtca aatgtaaact cgatgccact aaatgttgaa ctaaatagat tattcccttt
     1321 tttaaaagat gccattatcg tccatatcac tggcccaagc acaagaatgg agttgcagat
     1381 taaaaaaaga taaactaaga gcgttgatat tttacgtaaa aatttatcca ttgtcacatc
     1441 cctacatcat ctttaaatga tttcatccgt ctgaattgcc atattgctat aaccgataag
     1501 aagataa
//
LOCUS       DAZKHF020000495         1506 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2201S31241
            SAMN43267800-rid25106413.denovo.498, whole genome shotgun sequence.
ACCESSION   DAZKHF020000495 DAZKHF020000000
VERSION     DAZKHF020000495.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267800
            Sequence Read Archive: SRR30307263
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1506)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1506)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 1506)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350581.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350581.2
            Genome Coverage       :: 151x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:21:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,652
            CDSs (total)                      :: 5,557
            Genes (coding)                    :: 5,320
            CDSs (with protein)               :: 5,320
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 237
            CDSs (without protein)            :: 237
            Pseudo Genes (ambiguous residues) :: 0 of 237
            Pseudo Genes (frameshifted)       :: 102 of 237
            Pseudo Genes (incomplete)         :: 130 of 237
            Pseudo Genes (internal stop)      :: 52 of 237
            Pseudo Genes (multiple problems)  :: 41 of 237
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1506
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267800-rid25106413.denovo.498"
                     /serotype="O 26:H11"
                     /isolate="2201S31241"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-01"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            complement(36..686)
                     /locus_tag="ACHOSC_RS25145"
                     /old_locus_tag="pgaptmp_005029"
     CDS             complement(36..686)
                     /locus_tag="ACHOSC_RS25145"
                     /old_locus_tag="pgaptmp_005029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017441421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LexA family protein"
                     /protein_id="HGU4550777.1"
                     /translation="MNTQLMGERIRARRKELKIRQAALGKMVGVSNVAISQWERSETE
                     PNGENLLALAKALQCSPDYLLKGEDSLSNIAYHSRHDPRGSYPLISWVSAGCWMEAVE
                     PYHRRAIDNWYDTTVDCSEDSFWLDVKGDSMTAPAGLSIPEGMIILVDPEVEPRNGKL
                     VVAKLEGENEATFKKLVIDAGRKFLKPLNPQYPMIEINGNCKIIGVVVDAKLANLP"
     gene            767..952
                     /locus_tag="ACHOSC_RS25150"
                     /old_locus_tag="pgaptmp_005030"
     CDS             767..952
                     /locus_tag="ACHOSC_RS25150"
                     /old_locus_tag="pgaptmp_005030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000276885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cro/CI family transcriptional regulator"
                     /protein_id="HGU4550778.1"
                     /translation="MYKKDVIDHFGTQRAVAKALGISDAAVSQWKEVIPEKDAYRLEI
                     VTAGALKYQESAYRQAA"
     gene            1062..1358
                     /locus_tag="ACHOSC_RS25155"
                     /old_locus_tag="pgaptmp_005031"
     CDS             1062..1358
                     /locus_tag="ACHOSC_RS25155"
                     /old_locus_tag="pgaptmp_005031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CII family transcriptional regulator"
                     /protein_id="HGU4550779.1"
                     /translation="MAQASYSKLTQREIDRAETDLLINLSTLTQRGLAKMIGCHESKI
                     SRTDWRFIASVLCAFGMASDISPISRAFKYALDEITKKKSPVAAGDSKQIDMQF"
     gene            1391..>1506
                     /locus_tag="ACHOSC_RS25160"
                     /old_locus_tag="pgaptmp_005032"
     CDS             1391..>1506
                     /locus_tag="ACHOSC_RS25160"
                     /old_locus_tag="pgaptmp_005032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication protein"
                     /protein_id="HGU4550780.1"
                     /translation="MTNTAKILNFGRGNFAEQERNVADLDDGYARLSNMLIEA"
BASE COUNT          433 a          338 c          334 g          401 t
ORIGIN      
        1 tttttaacgg gaaataaaaa aggggcgaaa gccccttaag gaaggtttgc tagcttggca
       61 tcaacgacaa cgccaatgat tttacagttc ccattgattt caatcattgg gtattgtgga
      121 ttgagtggtt tcaggaattt tctaccggca tcaataacta actttttgaa tgtcgcctcg
      181 ttttctcctt caagtttggc gactaccagc tttccattac gtggttcgac ttctgggtcg
      241 acgagaataa tcatcccttc aggaatactc agtcctgccg gggcagtcat tgaatcacct
      301 ttaacgtcga gccaaaaaga gtcttcagaa caatctaccg ttgtgtcgta ccagttatct
      361 attgcacgcc tatgatatgg ctctacagct tccatccaac atcctgcgct tacccaacta
      421 attagaggat acgaacctct tggatcatgc ctgctgtgat aggcaatgtt tgaaagacta
      481 tcctctcctt tcaacaggta atcaggggag cactgcaaag ccttggctaa ggccaatagg
      541 ttttcgccat tgggctcagt ttcagatcgc tcccattggg aaatagcaac attagacacg
      601 ccaaccatct tgccaagggc agcctgccta atcttgagtt cttttctgcg agcgcgaata
      661 cgctcaccca tcagttgtgt attcatagtt aagacatctt aaataaactt gacttaagat
      721 tcctttggtg gataatttaa gtgttcttta atttcggagc gagtctatgt acaaaaaaga
      781 tgttattgac cacttcggaa cccagcgtgc tgttgctaaa gcactaggca ttagcgatgc
      841 agcagtctct cagtggaaag aagttatccc agagaaagac gcctatcgat tggaaatcgt
      901 tacagctggc gccctgaagt atcaagaaag tgcttaccgc caagcggcat aagtaaattg
      961 ctctttaaca gttctggcct ttcacctcta accgggtgag caaacatcag cggcaaatcc
     1021 actgggtgtg ccgctatata tcaatatagg aaaattaaca aatggcacaa gcaagttaca
     1081 gcaagctaac acagcgagaa attgatcgcg cagaaacaga tttactcatc aacctgtcaa
     1141 cgcttaccca gcgcggtctg gcaaagatga ttggctgtca tgaatcgaag ataagcagaa
     1201 cggactggag atttattgct tcggtcttgt gtgctttcgg aatggcatca gacatcagtc
     1261 cgattagcag ggcttttaag tatgcgcttg atgaaatcac aaagaaaaaa tccccggtgg
     1321 ccgccgggga ctctaagcaa attgatatgc aattctgagg gaattactgg atcaatccac
     1381 aggagtcatt atgacaaata cagcaaaaat actcaacttc ggcagaggta actttgccga
     1441 acaggagcgt aatgtggcag atctcgatga tggttacgcc agactatcaa atatgctgat
     1501 tgaggc
//
LOCUS       DAZKHF020000496         1501 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2201S31241
            SAMN43267800-rid25106413.denovo.499, whole genome shotgun sequence.
ACCESSION   DAZKHF020000496 DAZKHF020000000
VERSION     DAZKHF020000496.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267800
            Sequence Read Archive: SRR30307263
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1501)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1501)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 1501)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350581.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350581.2
            Genome Coverage       :: 151x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:21:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,652
            CDSs (total)                      :: 5,557
            Genes (coding)                    :: 5,320
            CDSs (with protein)               :: 5,320
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 237
            CDSs (without protein)            :: 237
            Pseudo Genes (ambiguous residues) :: 0 of 237
            Pseudo Genes (frameshifted)       :: 102 of 237
            Pseudo Genes (incomplete)         :: 130 of 237
            Pseudo Genes (internal stop)      :: 52 of 237
            Pseudo Genes (multiple problems)  :: 41 of 237
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1501
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267800-rid25106413.denovo.499"
                     /serotype="O 26:H11"
                     /isolate="2201S31241"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-01"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            <1..1315
                     /locus_tag="ACHOSC_RS25165"
                     /old_locus_tag="pgaptmp_005033"
     CDS             <1..1315
                     /locus_tag="ACHOSC_RS25165"
                     /old_locus_tag="pgaptmp_005033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001536548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="AAA domain-containing protein"
                     /protein_id="HGU4550781.1"
                     /translation="RFIKRFPVIGSSTHSIINSIGKGALLDYVIIDEASQQDIVPGIL
                     GLGCARNVIVVGDRKQLPHVPVLLPNSPSPPAEYYNCEKYSLLDSVCMLFRNMVPVTL
                     LKEHYRCHPKIIQFCNKQFYDNALIPLTLDSGEASLSLIITAKGNHTRNFSNLRELES
                     LEGHYWDEESSRGYIAPYNAQVNLAETVLPADFVKSTVHKFQGRECDEIVFSTVLDKK
                     RSSQHSRNIAFVDNPELVNVAVSRARNKFTLVTGNDVFERHAGHIAALIRYIKYYADD
                     GEIFESPVISAFDLLYSEYDKSLERLNSRLNSNDSHFKSEQIVACLLRDILSQDSYRS
                     MMFHSQIALNQLVLLERGDFTHREQLFMRNRASCDFVVYYKVGKTPLGVIEVDGGYHL
                     TSVQAERDELKNSILKKCGLPLLRLRTIDSDIEGKLGAFLSGLTG"
BASE COUNT          415 a          280 c          365 g          441 t
ORIGIN      
        1 ccgttttata aagcgttttc cggttattgg tagcagcacg cattctatta ttaactctat
       61 cggtaagggg gctcttcttg attatgtgat tattgatgaa gcctcacagc aggatattgt
      121 tccgggcatt ctgggactgg gatgtgcccg taatgtaatc gttgtagggg atcgaaagca
      181 acttccacat gttcctgtat tactcccgaa cagcccatct ccaccggcag aatactataa
      241 ttgtgagaaa tatagtctac ttgattcggt ttgtatgttg ttcaggaata tggtgccagt
      301 aactcttctt aaagagcatt atcgctgtca tcctaaaatc attcaatttt gtaataagca
      361 gttttatgat aatgctctga taccgctaac gcttgattca ggtgaagcct cattatctct
      421 gatcatcacg gcaaaaggaa accatacccg taatttttca aatctcaggg agcttgaatc
      481 tctggaagga cattactggg atgaggaaag tagcagggga tatatcgccc catataatgc
      541 acaggttaat ctggctgaaa cagtgctgcc tgctgatttt gtgaaatcga cggtgcataa
      601 atttcaggga cgggagtgcg atgaaattgt gttttccact gtgctggata agaagcgcag
      661 cagccagcac agtcggaaca tcgcgtttgt tgataatcct gaactggtga atgtcgctgt
      721 ttcaagggcc cgaaataaat ttacgctggt gactggtaat gatgtatttg aacgccacgc
      781 tggacatatt gcggcgctga ttcgttacat caaatactac gctgatgacg gagagatttt
      841 cgagagcccg gtcatatcag catttgattt gctgtacagt gagtatgaca aatcactgga
      901 acgactgaat agtagattga attcaaatga ttcacacttt aagtcagaac agattgtggc
      961 ctgcctgttg cgggatatcc tgtcgcagga tagttatcgg agtatgatgt ttcattcaca
     1021 aatagcgctg aatcagttgg tgttgctgga gcggggggat tttacacacc gtgaacagtt
     1081 attcatgcgt aaccgggcca gttgtgactt tgttgtttac tataaggtcg ggaagactcc
     1141 attaggggtt attgaagtgg atggaggtta tcatctgacc agtgttcagg ctgaacgtga
     1201 tgaacttaaa aacagtatac tgaagaaatg cggcctgcca ttgctaagat tacgtactat
     1261 agacagtgat atagaaggga agttgggagc gtttctgtca ggcctgacag gttaaagtct
     1321 gaataaaaaa tattgcagcg ggaggtctca aacctgattt ggtcatgtgc cacacacaat
     1381 tgtacgaacc ccatggacac aggcttcttt gttttggtta catcaacgta cgtattcctg
     1441 atacatataa aaataagtgc tattgctcaa agctaacagg gaatggttct gttgaataat
     1501 t
//
LOCUS       DAZKHF020000497         1492 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2201S31241
            SAMN43267800-rid25106413.denovo.501, whole genome shotgun sequence.
ACCESSION   DAZKHF020000497 DAZKHF020000000
VERSION     DAZKHF020000497.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267800
            Sequence Read Archive: SRR30307263
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1492)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1492)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 1492)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350581.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350581.2
            Genome Coverage       :: 151x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:21:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,652
            CDSs (total)                      :: 5,557
            Genes (coding)                    :: 5,320
            CDSs (with protein)               :: 5,320
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 237
            CDSs (without protein)            :: 237
            Pseudo Genes (ambiguous residues) :: 0 of 237
            Pseudo Genes (frameshifted)       :: 102 of 237
            Pseudo Genes (incomplete)         :: 130 of 237
            Pseudo Genes (internal stop)      :: 52 of 237
            Pseudo Genes (multiple problems)  :: 41 of 237
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1492
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267800-rid25106413.denovo.501"
                     /serotype="O 26:H11"
                     /isolate="2201S31241"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-01"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            <1..382
                     /locus_tag="ACHOSC_RS25170"
                     /old_locus_tag="pgaptmp_005034"
     CDS             <1..382
                     /locus_tag="ACHOSC_RS25170"
                     /old_locus_tag="pgaptmp_005034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009453323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="lysis protein"
                     /protein_id="HGU4550782.1"
                     /translation="SWAVNHYRDNAIAYKEQRDKATSIIADMQKRQRDVAELDARYTK
                     ELADANATIESLRADVSAGRKRLQVSATCAKSTTGASSMGDGESPGLTSDAELNYYRL
                     RGGIDKITAQVNYLQEYIRTQCLK"
     gene            532..1149
                     /locus_tag="ACHOSC_RS25175"
                     /old_locus_tag="pgaptmp_005035"
     CDS             532..1149
                     /locus_tag="ACHOSC_RS25175"
                     /old_locus_tag="pgaptmp_005035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000881338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rha family transcriptional regulator"
                     /protein_id="HGU4550783.1"
                     /translation="MLASLGGLVSCAAKVHFKEAGNAMNELIANHDFDFRQLVTAAEG
                     QPVTDTFQIARAFGKRHQHVIRAIKCLRCSEEFSTTHFWAVEKINDLGIFDKKQIYYR
                     MDFSGFVMLVMGFNGAKADAVKEAYINAFNWMSAELRKYSESYEAERNAVMLEYMKEK
                     DVASMSGRLLNRWGRTKKPQLLAKLERLERQGQFLLPGFDKGIQA"
     gene            complement(1337..>1492)
                     /locus_tag="ACHOSC_RS25180"
                     /old_locus_tag="pgaptmp_005036"
     CDS             complement(1337..>1492)
                     /locus_tag="ACHOSC_RS25180"
                     /old_locus_tag="pgaptmp_005036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001307652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlcI/YnfO family protein"
                     /protein_id="HGU4550784.1"
                     /translation="NIRFPNQMIEQINIALEQKGSGNFSAWVIEACRRRLTSEKRAYT
                     SIQSDDG"
BASE COUNT          408 a          336 c          376 g          372 t
ORIGIN      
        1 gtcatgggct gttaatcatt accgtgataa cgccatcgcc tacaaagagc agcgcgataa
       61 agccacatcc atcatcgctg atatgcagaa gcggcaacgt gatgtagcag aacttgacgc
      121 cagatacaca aaggagcttg ctgatgctaa tgcgactatc gaaagtctcc gtgctgatgt
      181 ttctgctggg cgtaagcgcc tgcaagtctc cgccacctgt gcaaagtcaa cgaccggagc
      241 cagcagcatg ggcgatggag aaagcccagg acttacatca gatgctgaac tcaattatta
      301 ccgtctccga ggtggaatcg acaagataac cgcgcaggtt aactacctgc aggaatacat
      361 caggacgcag tgcttaaaat aattttaatt tcactgaaat ttaacaagtg actttcagga
      421 aaatgcctcg cagatgcggg gcatttttgt accggtattt caccgcgcac cgcagcgcac
      481 aataaacacc gaacctgacc ctttggaatg ggcctttgag gataccagtt agtgctggcg
      541 agcctcggtg ggctggtttc ctgtgcggca aaggttcatt tcaaagaagc aggcaacgcc
      601 atgaatgaat taattgcgaa tcatgacttc gactttcgcc agttagttac cgcagcagaa
      661 ggtcaaccgg taactgacac cttccagatt gccagggcat ttggtaaacg ccatcagcat
      721 gtgattaggg ctattaaatg tttgagatgt tctgaggaat tctcgacaac ccatttttgg
      781 gccgtcgaga aaatcaatga cttaggtatt tttgacaaga aacagattta ctaccgcatg
      841 gactttagtg gcttcgttat gctggttatg ggatttaacg gggcaaaagc cgatgctgtt
      901 aaagaagcct atatcaatgc gtttaactgg atgtcagcag aactccgtaa gtacagcgaa
      961 agttatgaag cagaacgtaa cgccgtaatg ctggagtaca tgaaagagaa ggatgtcgcc
     1021 agcatgtcag gccgtctgct caatcgctgg gggagaacga aaaaacctca attgcttgca
     1081 aagctggaac gtctggagag acagggacag tttttattac cgggattcga taaaggtatt
     1141 caagcctgac acattatgcg ctgtatcgtc gccgtattcc cgcattaacc atgaccgtag
     1201 cccgacgggg aattccttct gcgtgagtgt gcgggaataa tcaaaaacga tgcacaccgg
     1261 gttttactgt gctgacagac gcagggttac cctcatagtc gcttttccgg tgcgatggtg
     1321 gaagaaaccg ggatgttcat ccatcatcac tttggattga tgtatatgct ctcttttctg
     1381 acgttagtct ccgacggcag gcttcaatga cccaggctga gaaattccca gacccttttt
     1441 gctcaagagc gatgttaatt tgttcaatca tttggttagg aaagcggatg tt
//
LOCUS       DAZKHF020000498         1491 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2201S31241
            SAMN43267800-rid25106413.denovo.502, whole genome shotgun sequence.
ACCESSION   DAZKHF020000498 DAZKHF020000000
VERSION     DAZKHF020000498.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267800
            Sequence Read Archive: SRR30307263
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1491)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1491)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 1491)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350581.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350581.2
            Genome Coverage       :: 151x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:21:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,652
            CDSs (total)                      :: 5,557
            Genes (coding)                    :: 5,320
            CDSs (with protein)               :: 5,320
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 237
            CDSs (without protein)            :: 237
            Pseudo Genes (ambiguous residues) :: 0 of 237
            Pseudo Genes (frameshifted)       :: 102 of 237
            Pseudo Genes (incomplete)         :: 130 of 237
            Pseudo Genes (internal stop)      :: 52 of 237
            Pseudo Genes (multiple problems)  :: 41 of 237
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1491
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267800-rid25106413.denovo.502"
                     /serotype="O 26:H11"
                     /isolate="2201S31241"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-01"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            complement(<1..>1491)
                     /locus_tag="ACHOSC_RS25185"
                     /old_locus_tag="pgaptmp_005037"
     CDS             complement(<1..>1491)
                     /locus_tag="ACHOSC_RS25185"
                     /old_locus_tag="pgaptmp_005037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310088.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RHS repeat-associated core domain-containing
                     protein"
                     /protein_id="HGU4550785.1"
                     /translation="GERQTVENPETGELLWQHETTHAYNEQGLANRVTPDSLPPVEWL
                     TYGSGYLAGMKLGDTPLLEYTRDRMHRETVRSFGSMAGSNAAYKLTSTYTPAGQLQSQ
                     HLNSLVYDRDYGWNDNGDLVRISGPRQTREYGYSATGRLESVRTLAPDLDIRIPYATD
                     PAGNRLPDPELHPDSTLTAWPDNRIAEDAHYVYHYDEYGRLTEKTDLIPAGVIRTDDE
                     RTHHYHYDSQHRLVFYTRIQHGEPLVESRYLYDPLGRRMAKRVWRRERDLTGWMSLSR
                     KPEMTWYGWDGDRLTTVQTDTTRIQTVYQPGSFAPLIRIETDNGEREKAQRRSLAEKL
                     QQEGSEDGHGVVFPAELVRLLDRLEEEIRADRVSSESRAWLAQCGLTVEQLARQVEPE
                     YTPARKVHFYHCDHRGLPLALISEDGNTAWRGEYDEWGNQLNEENPYYLHQPYRLPGQ
                     QHDEESGLYYNRNRYYDPLQGRYITQDPIGLAGGWNLYNYPLNPIIR"
BASE COUNT          209 a          505 c          429 g          348 t
ORIGIN      
        1 ccttattatc ggattcagtg ggtaattata caggttccat ccccccgcca gcccaatggg
       61 gtcttgtgta atatacctcc cctgtagcgg gtcatagtac cggttccggt tatagtacag
      121 ccctgattcc tcatcatgct gctgccccgg cagacggtat ggctggtgca ggtaatacgg
      181 gttctcttca ttaagctggt tgccccattc atcatactcc ccgcgccacg ccgtattgcc
      241 gtcttcgctg atgagcgcca gcggcaggcc ccggtggtcg cagtggtaaa aatgaacttt
      301 tcgcgccggt gtgtattccg gctccacctg tctggccagt tgctccaccg tcagcccgca
      361 ctgcgcaagc cacgcccggc tttcactgct cacgcggtct gcccggattt cttcctccag
      421 cctgtccagc agccgcacca gttcagccgg aaataccacg ccgtgcccgt cctcgctccc
      481 ttcctgctgc agcttctccg ccaggctgcg gcgctgcgct ttctcccgct cgccgttgtc
      541 tgtttcgatg cggatgagcg gcgcgaagct ccccggctga tataccgtct ggatacgcgt
      601 ggtgtcggtc tgtaccgtcg tcagccggtc gccgtcccac ccgtaccacg tcatctccgg
      661 tttacgcgac agcgacatcc accccgtcag gtcacgctcc cgccgccata cccgtttcgc
      721 cattcgccgt cccagcgggt cgtagaggta gcggctctcg accagtggct cgccatgctg
      781 tatccgcgtg tagaacacca gacggtgctg gctgtcgtag tggtagtggt gggtccgctc
      841 gtcgtccgtc cggataacac ccgccgggat aaggtccgtc ttctccgtca gtctgccgta
      901 ttcatcatag tggtaaacat agtgcgcatc ctctgcgatg cggttatccg gccacgccgt
      961 gagggtactg tccgggtgca gctccgggtc cggcaggcgg tttcccgccg ggtcggtggc
     1021 atacgggatg cggatgtcca ggtccggtgc gagggtacgc acgctctcca gcctgcccgt
     1081 ggcgctgtag ccgtattccc gcgtctgtcg cgggccgctg atgcgcacca ggtcgccgtt
     1141 gtcattccac ccgtagtcac ggtcatacac caggctgttc aggtgctggc tctgtaactg
     1201 gcctgcgggg gtgtatgtgc tggtcagttt atatgcggca ttactgcctg ccatgctgcc
     1261 aaagctgcgc accgtctccc ggtgcatgcg gtcgcgcgtg tactccagca gcggcgtgtc
     1321 gccaagcttc atgcccgcca gataaccgct gccgtaggtc agccactcca ccggcggcag
     1381 gctgtccggc gtgacgcggt ttgccagccc ctgttcgttg taggcgtgtg ttgtctcatg
     1441 ctgccacagc agttcccccg tctccgggtt ctccaccgtc tgccgttcgc c
//
LOCUS       DAZKHF020000499         1474 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2201S31241
            SAMN43267800-rid25106413.denovo.503, whole genome shotgun sequence.
ACCESSION   DAZKHF020000499 DAZKHF020000000
VERSION     DAZKHF020000499.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267800
            Sequence Read Archive: SRR30307263
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1474)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1474)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 1474)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350581.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350581.2
            Genome Coverage       :: 151x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:21:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,652
            CDSs (total)                      :: 5,557
            Genes (coding)                    :: 5,320
            CDSs (with protein)               :: 5,320
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 237
            CDSs (without protein)            :: 237
            Pseudo Genes (ambiguous residues) :: 0 of 237
            Pseudo Genes (frameshifted)       :: 102 of 237
            Pseudo Genes (incomplete)         :: 130 of 237
            Pseudo Genes (internal stop)      :: 52 of 237
            Pseudo Genes (multiple problems)  :: 41 of 237
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1474
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267800-rid25106413.denovo.503"
                     /serotype="O 26:H11"
                     /isolate="2201S31241"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-01"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            complement(<1..247)
                     /locus_tag="ACHOSC_RS25190"
                     /old_locus_tag="pgaptmp_005038"
     CDS             complement(<1..247)
                     /locus_tag="ACHOSC_RS25190"
                     /old_locus_tag="pgaptmp_005038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001556209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative zinc ribbon protein"
                     /protein_id="HGU4550786.1"
                     /translation="MRILNCYMANDSKGHFVTAKEAAKHNRQDVLCCVSCGCPLTLKR
                     GNDGQPPWFEHDQMTVAAKILLRCTWLDPAEKEARRLH"
     gene            433..1401
                     /locus_tag="ACHOSC_RS25195"
                     /old_locus_tag="pgaptmp_005039"
     CDS             433..1401
                     /locus_tag="ACHOSC_RS25195"
                     /old_locus_tag="pgaptmp_005039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003031967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS110 family transposase"
                     /protein_id="HGU4550787.1"
                     /translation="MSQPGSLCVDIDVSKAILDIAASSAIEQFSVGNDSDGFDAIIAE
                     LRMYAIALVLMKATGGLETAIVCALQAEGFELAVVNPRQARDFARAMGYLAKTDSIDA
                     RVLSQMAEVLNRHPERERFIRALPDTECQVLTAMVVRRRQLITMLVAERNRLHPAHPL
                     NRKSINIIIKALEDELARLDADMNSHIHSHFKELAKRLSSIKGVGTMTAAALLAEVPE
                     QGNLSRREISVLVGMVPVNRDSGTMRGRRTIFGGRAGVRTALYMAALVATRFNPVIKV
                     FYMRLLAAGKPKKVALVTCIRKLLTILNAMLRKNEEWNESYHHVAP"
BASE COUNT          368 a          363 c          386 g          357 t
ORIGIN      
        1 gatgcaaacg acgggcctct ttctctgccg ggtcaagcca ggtgcatcgc agcaggattt
       61 tcgcagcaac agtcatctgg tcatgttcaa accacggcgg ttgtccgtca tttccccgct
      121 taagtgttaa cggacaacca caggacacac agcacaaaac gtcctgtcgg ttgtgcttcg
      181 cggcttcttt cgccgtaaca aaatggcctt tgctgtcatt cgccatatag cagttcagaa
      241 ttcgcatatc tcctccttgt atcttgacaa ttggttaaaa taactgtgat tagatttggc
      301 aaggtgtatg cagcccgatc cctcaagggg ttcatagccc gtagctcagc gaatggcgca
      361 tacactgagc cactcatctg aaacccgaac agttgtcagt acgccataac gcatttgcaa
      421 ggatgggtaa tcatgagtca gccaggttca ctttgtgtgg atatcgatgt ttcgaaagcg
      481 atactggata ttgccgccag cagcgctatt gaacaatttt ctgtcggcaa tgacagtgat
      541 ggctttgacg ccatcattgc tgagttgaga atgtacgcca ttgcgctggt tttaatgaaa
      601 gccactggag gacttgaaac cgccatagtc tgtgcgcttc aggcagaagg ttttgagctt
      661 gccgtggtta accccagaca agctcgtgac tttgcccgcg ccatgggata cctcgcgaaa
      721 actgatagta ttgatgccag agtgctttcc cagatggctg aggttctcaa ccggcatccg
      781 gagcgggaac gctttatcag agcactgccg gatactgaat gtcaggttct cactgcaatg
      841 gtagttcgta ggcggcagtt gattacgatg ctggttgccg agagaaaccg gttgcaccct
      901 gctcatccat tgaacaggaa aagcattaat atcatcatca aagcactgga agacgagctt
      961 gcgcgactag atgctgacat gaacagccat atccatagcc acttcaaaga gcttgctaag
     1021 cgactgagca gtattaaagg tgttggcacc atgacggctg ctgcgctgct ggcggaagta
     1081 cccgaacagg gtaacctctc caggcgtgaa atcagtgtgc ttgtcggtat ggttccagta
     1141 aaccgggact cgggcacgat gagagggcga cgaaccatct ttggcggcag ggctggtgtg
     1201 cgaacagccc tgtacatggc agcgcttgtc gcaacccgct ttaacccggt aataaaggtg
     1261 ttttacatgc gactgcttgc cgcaggaaaa cccaaaaaag tggcgctggt tacctgtata
     1321 cgcaagctgc tgactatcct gaacgcaatg ctcagaaaga acgaagaatg gaatgaatcg
     1381 taccatcacg ttgctccata atttttacgt tcaagacagt tgctgagctg tccggggaag
     1441 actgaaacct ccggtctggc agtgtgtgca gtac
//
LOCUS       DAZKHF020000500         1472 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2201S31241
            SAMN43267800-rid25106413.denovo.504, whole genome shotgun sequence.
ACCESSION   DAZKHF020000500 DAZKHF020000000
VERSION     DAZKHF020000500.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267800
            Sequence Read Archive: SRR30307263
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1472)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1472)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 1472)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350581.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350581.2
            Genome Coverage       :: 151x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:21:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,652
            CDSs (total)                      :: 5,557
            Genes (coding)                    :: 5,320
            CDSs (with protein)               :: 5,320
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 237
            CDSs (without protein)            :: 237
            Pseudo Genes (ambiguous residues) :: 0 of 237
            Pseudo Genes (frameshifted)       :: 102 of 237
            Pseudo Genes (incomplete)         :: 130 of 237
            Pseudo Genes (internal stop)      :: 52 of 237
            Pseudo Genes (multiple problems)  :: 41 of 237
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1472
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267800-rid25106413.denovo.504"
                     /serotype="O 26:H11"
                     /isolate="2201S31241"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-01"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            <1..132
                     /locus_tag="ACHOSC_RS25200"
                     /old_locus_tag="pgaptmp_005040"
     CDS             <1..132
                     /locus_tag="ACHOSC_RS25200"
                     /old_locus_tag="pgaptmp_005040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000245530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdaE family protein"
                     /protein_id="HGU4550788.1"
                     /translation="KGIEESKSIKRPLHFMRGVIPTTEMKKYCSENCAEKDQMAHEL"
     gene            206..481
                     /locus_tag="ACHOSC_RS25205"
                     /old_locus_tag="pgaptmp_005041"
     CDS             206..481
                     /locus_tag="ACHOSC_RS25205"
                     /old_locus_tag="pgaptmp_005041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4550789.1"
                     /translation="MITNYEVTVVTTDDIVHEVNLEGKRIGYVIKTENKETPFTVVDI
                     DGPSGNVKTLDEGVTKMSLVHIGKNLPAEKKAGFLATLIAMKLKGEI"
     gene            580..>1472
                     /locus_tag="ACHOSC_RS25210"
                     /old_locus_tag="pgaptmp_005042"
     CDS             580..>1472
                     /locus_tag="ACHOSC_RS25210"
                     /old_locus_tag="pgaptmp_005042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000105095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051908 - double-stranded DNA 5'-3' DNA
                     exonuclease activity [Evidence IEA];
                     GO_process: GO:0090304 - nucleic acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RecE family exodeoxyribonuclease"
                     /protein_id="HGU4550790.1"
                     /translation="MSTKPLFLLRKAKKSSGEPDVVLWASDDFESTCATLDYLIVKSG
                     KKLSNYFKAVATNFPVVNDLPPEGEIDFTWSERYQLSKDSMTWELKPGAAPDDVHHQE
                     DAPETEEPTGGQEENAQADAHEDCQDCEVSVATLRFTQRLLHIFTYAAGDRKYLHHAT
                     REQRKHITALEMDQENSYVQNLLLAIRSMAEPTTLDNAALLRLTDAIKAVFSITKKHQ
                     PYEFKNFISAWLDTEHIDRGLLTKEWRKGNRVSRITRTASGANAGGGNLTDRGEGFVH
                     DLTSLARDVATGVLARSMDVD"
BASE COUNT          466 a          337 c          340 g          329 t
ORIGIN      
        1 aaaggaattg aagaaagcaa aagcattaaa agaccacttc atttcatgcg tggagttatc
       61 ccaacgacgg aaatgaaaaa atattgtagt gaaaattgtg ccgaaaaaga ccagatggca
      121 cacgaacttt aattaactga ctatgcgaaa ctgaatttat gccagcaatg gcagggattc
      181 gctcaacctt aattaaggag aaaaaatgat taccaattat gaagtcactg ttgtaactac
      241 cgatgacatt gttcacgagg ttaatctgga aggaaagcgt attggctacg tgattaaaac
      301 agaaaataaa gaaaccccat tcactgtggt tgatattgac ggtccatcag gcaacgtaaa
      361 aacacttgat gaaggtgtca caaaaatgag tctggttcac atcggaaaga atctgcccgc
      421 agaaaaaaaa gccggatttc tggcaactct gattgcaatg aaattaaaag gtgaaatctg
      481 aaaaaagaaa gcctgcacaa cgtgcaggcc tgagtgaaga acctgggaca tttattcatc
      541 actcgcagta attttaatct gagttgaggt taaaaaacaa tgagcacaaa accactcttc
      601 cttttacgga aagcgaaaaa atcatccggt gaacctgacg tcgtcctgtg ggcaagcgac
      661 gattttgaat caacctgtgc cactctggac tacctgatcg ttaagtcagg taaaaaactg
      721 agcaactatt ttaaagctgt tgccacaaat tttcctgtcg ttaatgacct tccccctgaa
      781 ggtgagatcg attttacctg gagtgaacgc tatcaactca gcaaagactc catgacctgg
      841 gaactaaaac cgggagcagc gccagacgac gttcaccatc aggaggatgc accggaaacc
      901 gaagaaccga cgggaggcca ggaagaaaac gcgcaggcag acgcccacga ggattgccag
      961 gattgcgaag tctctgtagc cactttgcgg ttcacacagc gtcttctgca catttttacg
     1021 tatgcagccg gggatcgaaa atacctgcat catgccaccc gtgaacaacg caaacacatt
     1081 actgctcttg agatggatca ggaaaacagc tatgtccaga atctgctgtt ggccatacgc
     1141 agcatggcag aaccgacaac tctggataat gccgccctgc tccgcctgac tgatgcaatt
     1201 aaggcagtgt tctctatcac gaaaaaacat cagccctatg aatttaagaa tttcatttca
     1261 gcctggctgg ataccgaaca cattgatcgc ggtcttctga caaaagaatg gcgaaaaggg
     1321 aatcgtgttt cacgcatcac tcgcacggct tccggtgcta atgctggcgg cgggaacctc
     1381 accgatcgcg gcgaaggttt cgtccacgat ctgacatcac tggcgcgcga tgtagccact
     1441 ggcgtactgg cccgttcaat ggacgtggac at
//
LOCUS       DAZKHF020000501         1471 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2201S31241
            SAMN43267800-rid25106413.denovo.505, whole genome shotgun sequence.
ACCESSION   DAZKHF020000501 DAZKHF020000000
VERSION     DAZKHF020000501.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267800
            Sequence Read Archive: SRR30307263
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1471)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1471)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 1471)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350581.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350581.2
            Genome Coverage       :: 151x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:21:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,652
            CDSs (total)                      :: 5,557
            Genes (coding)                    :: 5,320
            CDSs (with protein)               :: 5,320
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 237
            CDSs (without protein)            :: 237
            Pseudo Genes (ambiguous residues) :: 0 of 237
            Pseudo Genes (frameshifted)       :: 102 of 237
            Pseudo Genes (incomplete)         :: 130 of 237
            Pseudo Genes (internal stop)      :: 52 of 237
            Pseudo Genes (multiple problems)  :: 41 of 237
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1471
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267800-rid25106413.denovo.505"
                     /serotype="O 26:H11"
                     /isolate="2201S31241"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-01"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            1..171
                     /locus_tag="ACHOSC_RS25215"
                     /old_locus_tag="pgaptmp_005043"
                     /pseudo
     CDS             1..171
                     /locus_tag="ACHOSC_RS25215"
                     /old_locus_tag="pgaptmp_005043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000491542.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="secretion protein EspS"
     gene            263..753
                     /locus_tag="ACHOSC_RS25220"
                     /old_locus_tag="pgaptmp_005044"
                     /pseudo
     CDS             263..753
                     /locus_tag="ACHOSC_RS25220"
                     /old_locus_tag="pgaptmp_005044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612175.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3-like element IS2 family transposase"
     gene            753..902
                     /locus_tag="ACHOSC_RS25225"
                     /old_locus_tag="pgaptmp_005045"
     CDS             753..902
                     /locus_tag="ACHOSC_RS25225"
                     /old_locus_tag="pgaptmp_005045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF957 domain-containing protein"
                     /protein_id="HGU4550791.1"
                     /translation="MLIAWLQDNIDCESGIIFDNDEDKTDSAALLPCIEQAREARYFP
                     SVLSA"
     gene            1277..1391
                     /locus_tag="ACHOSC_RS25230"
                     /old_locus_tag="pgaptmp_005046"
                     /pseudo
     CDS             1277..1391
                     /locus_tag="ACHOSC_RS25230"
                     /old_locus_tag="pgaptmp_005046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001218818.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="integrase"
BASE COUNT          416 a          343 c          339 g          373 t
ORIGIN      
        1 tacaataaaa tttatctgcc aggggatggt tctgctggcc tacctcttct taagtgtgga
       61 gatgaatttt tatcaccaac tgatatcgtc aatcgtctgg tagaacataa tcttcatgaa
      121 gtagaagaca tcagattaac atcctgtcac tcagccaaca taacaaaaaa ctagactggc
      181 cccctgaatc tccagacaac cagtatcact taaataagtg atagtcttaa tactagtttt
      241 tagactagtc attggagaac agatgattga tgtcttaggg cctgagaaac gcagacggcg
      301 taccacacag gaaaagatcg caattgttca gcagagcttt gaaccgggga tgacggtctc
      361 cctcgttgcc cggcaacatg gtgtagcagc cagccagtta tttctctggc gtaagcaata
      421 ccaggaagga agtcttactg ctgtcgccgc cggagaacag gttgttcctg cctctgaact
      481 tgctgccgcc atgaagcaga ttaaagaact ccagcgcctg ctcggcaaga aaacgatgga
      541 aaatgaactc ctcaaagaag ccgttgaata tggacgggca aaaaagtgga tagcgcacgc
      601 gcccttattg cccggggatg gggagtaagc ttagtcagcc gttgtctccg ggtgtcgcgt
      661 gcgcagttgt acgtcattct cagacgaacc gatgactgga tggatggccg ccgcagtcgt
      721 cacactgatg atacggatgt gcttctccgt atatcctcat tgcgtggctg caggacaata
      781 tcgactgcga atcgggaatt atctttgaca acgatgagga taaaacggat tcggcagcac
      841 tgctgccctg tattgaacag gccagggaag ccaggtactt tccttctgtg ctctcagcct
      901 gatcccgagt gtgattccct ttcgatatca aaagggactg cgggtataat tgagggtgca
      961 tttttaatca aaccaaacaa attgcattaa tatcaatatg ataaaggaaa ttttcgattc
     1021 cgagtccggc accaaattca tatcaacgga cctccacgga ggtccgtttt tcgtttctgg
     1081 acgccacaat ttaagcgttc tgcctccaaa tcaattctcc cgaactcaac cagattctcc
     1141 ccacatcacc agcaatttgc gggcatatcc caattcggga aaatttgttc ctgagcgata
     1201 gcactaactg acgtgaaatg tcgtgcggcc ccgtgatgtt gttgaacgtc aaatgacgtc
     1261 atcaggagcg taacgcatcc ataaagcaca acaccggtct gaacgccgac tgatgagatt
     1321 ttctgaatga gaacaaagag aaatgtatca gtccgcttgc tcatgcaaag actaacaatc
     1381 cattaaaata gtaagcgctc cggacaattt tccatggatt attttctgaa catttttctt
     1441 tggcaaagat gatgaatttt gatggtaagg a
//
LOCUS       DAZKHF020000502         1466 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2201S31241
            SAMN43267800-rid25106413.denovo.506, whole genome shotgun sequence.
ACCESSION   DAZKHF020000502 DAZKHF020000000
VERSION     DAZKHF020000502.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267800
            Sequence Read Archive: SRR30307263
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1466)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1466)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 1466)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350581.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350581.2
            Genome Coverage       :: 151x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:21:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,652
            CDSs (total)                      :: 5,557
            Genes (coding)                    :: 5,320
            CDSs (with protein)               :: 5,320
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 237
            CDSs (without protein)            :: 237
            Pseudo Genes (ambiguous residues) :: 0 of 237
            Pseudo Genes (frameshifted)       :: 102 of 237
            Pseudo Genes (incomplete)         :: 130 of 237
            Pseudo Genes (internal stop)      :: 52 of 237
            Pseudo Genes (multiple problems)  :: 41 of 237
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1466
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267800-rid25106413.denovo.506"
                     /serotype="O 26:H11"
                     /isolate="2201S31241"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-01"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            complement(<1..270)
                     /locus_tag="ACHOSC_RS25235"
                     /old_locus_tag="pgaptmp_005047"
     CDS             complement(<1..270)
                     /locus_tag="ACHOSC_RS25235"
                     /old_locus_tag="pgaptmp_005047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001760115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4550792.1"
                     /translation="MKQQYQTRYEWLHESYQKWLTGFTRHAVSWGVCHPNIYYFHNLT
                     PGWVSFNGEKPEIAIVPQSLHRLIYGPDKRATPPLDDDLIVNLCTS"
     gene            complement(365..571)
                     /locus_tag="ACHOSC_RS25240"
                     /old_locus_tag="pgaptmp_005048"
     CDS             complement(365..571)
                     /locus_tag="ACHOSC_RS25240"
                     /old_locus_tag="pgaptmp_005048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020234041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HGU4550793.1"
                     /translation="MATPVSLMDDQMVDMAFITQLTGLTDKWFYKLIKDGGFPAPIKM
                     ERSSRWLKSEVEAWLQARIAQSRP"
     gene            1328..1453
                     /locus_tag="ACHOSC_RS25245"
                     /old_locus_tag="pgaptmp_005049"
                     /pseudo
     CDS             1328..1453
                     /locus_tag="ACHOSC_RS25245"
                     /old_locus_tag="pgaptmp_005049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000453335.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="Hha/YmoA family nucleoid-associated regulatory
                     protein"
BASE COUNT          378 a          341 c          415 g          332 t
ORIGIN      
        1 actggtgcat aaattcacta tcagatcatc atccaggggc ggtgtggccc gtttgtcagg
       61 gccataaatc agccggtgca ggctctgggg tacgatggca atctccggtt tttcaccatt
      121 gaatgacacc caaccgggcg tcagattatg gaagtagtag atattcggat gacacacgcc
      181 ccaggatacg gcgtgccggg tgaagccggt cagccatttc tggtagcttt cgtggagcca
      241 ttcgtagcgg gtctgatact gttgtttcat tttcatattc ctcacgggga tataacaggc
      301 agcagaatgc cgccggcagg ccggagatta cgtctgctgc gggtgaacgg ataacggtca
      361 gaaattacgg acgggactgt gcaatacgcg cctgcagcca ggcttccact tcacttttca
      421 gccagcggga gctgcgctcc attttgatgg gggcaggaaa gcccccatct ttgatgagtt
      481 tgtaaaacca cttatcggtt aagccggtaa gttgagtgat aaacgccatg tcgaccatct
      541 ggtcatccat cagcgaaaca ggggtagcca tgagaatgtt cctccggatg ttgttaaacc
      601 agccggtgtg gtcatgaaaa cagcgcgcca ccttcactgg cataacaacc ggggagaaac
      661 gtaaaaaata atcgggaatg tggctgtccg gagataaacg gggggattgc gatggcagag
      721 ccgtagcgga atcccgggtg gttgttgcga aaagatgctt ccgcacgcac gcgcgataaa
      781 ggagggagga ctttcgtgag acaaggggaa aactagcgtt ctgataattg cagagttgtt
      841 ttcggaggtg gttacagtga gtcagcccgt tcctggcgag gaggagactg atatcaccga
      901 tgggataagg ctgtctgatt ggtattataa atataagaag tcatcagaga gtaacgtaaa
      961 ggatttgcca gagtaagcta agagagtaaa tctctccggt aacagagcgc tgtgaatcgt
     1021 tcatgtatca gagtgcagat acatgaacgc tgatgcgtaa aatcagcagt gacacgccag
     1081 ctccccctgt gcccattcct gtataagaca tgaactgagc gtattaatgc tcctgccctg
     1141 agactgagcc ggtgagtgag gggctgagaa atccctcttg acactgctct aaaccagaaa
     1201 tacttccccg gttgttgcgg tgcgtccgga tccccgataa cggaacacta aggctgtatt
     1261 gcgggacacc tggtaagtgg cagcccgtaa tacgtcagga gacgcgggaa aagtgaattc
     1321 ccacacgcgt tataaactta cccttgcgga gctggaagcc tttaactctg ccgttgacaa
     1381 ccggctggca gaactgacaa tgaacaaact ttacgatcgc gcgccggctt ccgtctggaa
     1441 atatgtcacc tgaatattga agcagc
//
LOCUS       DAZKHF020000503         1464 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2201S31241
            SAMN43267800-rid25106413.denovo.507, whole genome shotgun sequence.
ACCESSION   DAZKHF020000503 DAZKHF020000000
VERSION     DAZKHF020000503.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267800
            Sequence Read Archive: SRR30307263
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1464)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1464)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 1464)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350581.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350581.2
            Genome Coverage       :: 151x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:21:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,652
            CDSs (total)                      :: 5,557
            Genes (coding)                    :: 5,320
            CDSs (with protein)               :: 5,320
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 237
            CDSs (without protein)            :: 237
            Pseudo Genes (ambiguous residues) :: 0 of 237
            Pseudo Genes (frameshifted)       :: 102 of 237
            Pseudo Genes (incomplete)         :: 130 of 237
            Pseudo Genes (internal stop)      :: 52 of 237
            Pseudo Genes (multiple problems)  :: 41 of 237
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1464
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267800-rid25106413.denovo.507"
                     /serotype="O 26:H11"
                     /isolate="2201S31241"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-01"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            complement(<1..1442)
                     /locus_tag="ACHOSC_RS25250"
                     /old_locus_tag="pgaptmp_005050"
     CDS             complement(<1..1442)
                     /locus_tag="ACHOSC_RS25250"
                     /old_locus_tag="pgaptmp_005050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415044.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative allantoin permease"
                     /protein_id="HGU4550794.1"
                     /translation="MEHQRKLFQQRGYSEDLLPKTQSQRTWKTFNYFTLWMGSVHNVP
                     NYVMVGGFFILGLSTFSIMLAIILSAFFIAAVMVLNGAAGSKYGVPFAMILRASYGVR
                     GALFPGLLRGGIAAIMWFGLQCYAGSLACLILIGKIWPGFLTLGGDFTLLGLSLPGLI
                     TFLIFWLVNVGIGFGGGKVLNKFTAILNPCIYIVFGGMAIWAISLVGIGPIFDYIPSG
                     IQKAENGGFLFLVVINAVVAVWAAPAVSASDFTQNAHSFREQALGQTLGLVVAYILFA
                     VAGVCIIAGASIHYGADTWNVLDIVQRWDSLFASFFAVLVILMTTISTNATGNIIPAG
                     YQIAAIAPTKLTYKNGVLIASIISLLICPWKLMENQDSIYLFLDIIGGMLGPVIGVMM
                     AHYFVVMRGQINLDELYTAPGDYKYYDNGFNLTAFSVTLVAVILSLGGKFIHFMEPLS
                     RVSWFVGVIVAFAAYALLKKRTTAEKTGEQ"
BASE COUNT          454 a          371 c          316 g          323 t
ORIGIN      
        1 ttttgctctc ctgttttttc tgctgttgta cgtttcttta ataaggcgta ggccgcaaag
       61 gcgacgatga cgccgacaaa ccatgaaaca cgcgataacg gttccataaa gtgaataaac
      121 ttaccgccaa gagataaaat aacggccacc agagttactg aaaacgcagt gaggttaaaa
      181 ccgttatcgt aatatttata atcgccaggt gcggtataca gttcatcaag attaatttgt
      241 ccgcgcatca ccacaaaata atgcgccatc atgacaccaa ttaccggacc aagcattccg
      301 ccgataatat cgaggaaaag ataaatgctg tcctgatttt ccattaattt ccacgggcag
      361 atcagcaagc tgataatact ggcaatcagt acgccgtttt tataggtcag ttttgtcggt
      421 gcaatggcgg caatctgata accggctgga ataatattac cggtcgcgtt agtggagata
      481 gttgtcatca gaataaccag taccgcaaag aacgaggcga acaggctgtc ccaacgctga
      541 acaatatcca gcacgttcca ggtatcagcg ccgtagtgaa tactggctcc ggcaataata
      601 cataccccgg cgaccgcaaa cagaatatag gccacaacta aacccagcgt ttgccccagc
      661 gcctgctcac gaaacgagtg ggcgttttgc gtaaagtcgg atgcgctcac cgccggtgcc
      721 gcccagaccg caactaccgc gttaatcacc accaggaaca ggaagccacc gttttctgct
      781 ttctgaatac cgctcggaat gtagtcaaag attggaccga tcccgaccag tgaaatcgcc
      841 caaatcgcca taccgccgaa aacgatatag atgcacgggt taagaatggc agtgaattta
      901 tttaaaactt tgccaccgcc aaaacctata ccaacgttga ccagccagaa gattaagaaa
      961 gtaattaagc ccggtagaga aaggcctaac agagtgaaat caccaccgag agttaaaaat
     1021 cccggccaga ttttgccaat cagaatcaag caggccagtg accccgcgta acattgcaaa
     1081 ccaaaccaca tgatggcggc aattccgccc cttaataatc cgggaaacag tgcaccacgt
     1141 acaccgtaag aagcacgcag gatcatggca aaaggcacac cgtatttact gcccgcagca
     1201 ccgtttaata ccattaccgc ggcaatgaaa aaggcgctga ggataattgc cagcataata
     1261 ctaaaggtag acaagccgag aataaaaaag ccgccgacca tcacataatt gggaacgtta
     1321 tgaaccgaac ccatccataa ggtaaaatag ttaaatgttt tccaggtccg ctggctttgc
     1381 gttttcggca atagatcttc gctatagccg cgttgctgga atagttttct ctgatgttcc
     1441 ataaatccct cttaatttca cgta
//
LOCUS       DAZKHF020000504         1461 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2201S31241
            SAMN43267800-rid25106413.denovo.508, whole genome shotgun sequence.
ACCESSION   DAZKHF020000504 DAZKHF020000000
VERSION     DAZKHF020000504.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267800
            Sequence Read Archive: SRR30307263
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1461)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1461)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 1461)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350581.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350581.2
            Genome Coverage       :: 151x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:21:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,652
            CDSs (total)                      :: 5,557
            Genes (coding)                    :: 5,320
            CDSs (with protein)               :: 5,320
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 237
            CDSs (without protein)            :: 237
            Pseudo Genes (ambiguous residues) :: 0 of 237
            Pseudo Genes (frameshifted)       :: 102 of 237
            Pseudo Genes (incomplete)         :: 130 of 237
            Pseudo Genes (internal stop)      :: 52 of 237
            Pseudo Genes (multiple problems)  :: 41 of 237
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1461
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267800-rid25106413.denovo.508"
                     /serotype="O 26:H11"
                     /isolate="2201S31241"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-01"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            <1..423
                     /locus_tag="ACHOSC_RS25255"
                     /old_locus_tag="pgaptmp_005051"
     CDS             <1..423
                     /locus_tag="ACHOSC_RS25255"
                     /old_locus_tag="pgaptmp_005051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6645 domain-containing protein"
                     /protein_id="HGU4550795.1"
                     /translation="AANQYVMAFYWPVSSLPQGVALTGDGGNNLLAAFYIQTDAKDLN
                     VMYHNAKVATNNLKLGTFGAFDNEWHTLAFRFAGNNSLQVTPVIDGQVGTPFTLTQSP
                     VSAFAADKLHVTDITRGATYPVLIDSIAVEVNSTDTAA"
     gene            559..738
                     /locus_tag="ACHOSC_RS25260"
                     /old_locus_tag="pgaptmp_005052"
     CDS             559..738
                     /locus_tag="ACHOSC_RS25260"
                     /old_locus_tag="pgaptmp_005052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1378 family protein"
                     /protein_id="HGU4550796.1"
                     /translation="MTFLNQLMLYFCTVVCVLYLLSGGYRAMRDVWRRQIDKRAAEKI
                     SASQSAGSKPEEPLI"
     gene            779..1051
                     /locus_tag="ACHOSC_RS25265"
                     /old_locus_tag="pgaptmp_005053"
     CDS             779..1051
                     /locus_tag="ACHOSC_RS25265"
                     /old_locus_tag="pgaptmp_005053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309238.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF826 domain-containing protein"
                     /protein_id="HGU4550797.1"
                     /translation="MSEITSLVTAEAVKEVLRSEEVRSALKQKLRHNLEARLDAEVDA
                     ILDELLGVQAEPPTEAGDTTAESGEVQPESPVADATEPQPESVMML"
     gene            1128..1343
                     /locus_tag="ACHOSC_RS25270"
                     /old_locus_tag="pgaptmp_005054"
     CDS             1128..1343
                     /locus_tag="ACHOSC_RS25270"
                     /old_locus_tag="pgaptmp_005054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0020002 - host cell plasma membrane
                     [Evidence IEA];
                     GO_function: GO:0042802 - identical protein binding
                     [Evidence IEA];
                     GO_function: GO:0140911 - pore-forming activity [Evidence
                     IEA];
                     GO_process: GO:0019835 - cytolysis [Evidence IEA];
                     GO_process: GO:0044659 - viral release from host cell by
                     cytolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class II holin family protein"
                     /protein_id="HGU4550798.1"
                     /translation="MYQMEKITTGVSYTTSAVGTGYWLLQLLDKVSPSQWVAIGVLGS
                     LLFGLLTYLTNLYFKIREDRRKAARGK"
     gene            1348..>1461
                     /locus_tag="ACHOSC_RS25275"
                     /old_locus_tag="pgaptmp_005055"
     CDS             1348..>1461
                     /locus_tag="ACHOSC_RS25275"
                     /old_locus_tag="pgaptmp_005055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1327 domain-containing protein"
                     /protein_id="HGU4550799.1"
                     /translation="MKKKYELVVKGINNYPDKITVTVALEIGGYPSLLLPDV"
BASE COUNT          389 a          327 c          412 g          333 t
ORIGIN      
        1 gctgcgaacc agtatgttat ggcgttttac tggccggtat cttcactgcc acagggcgtt
       61 gccctgaccg gagacggggg gaataacctg ctggcagcgt tctacatcca gacagatgca
      121 aaagacctga atgtgatgta ccacaatgcg aaagtggcga caaacaacct gaaactggga
      181 acctttggcg catttgataa cgaatggcat acgctggctt tccgctttgc cgggaataac
      241 agcctgcagg tgacgccggt tattgatggt caggttggca caccgttcac gctgacgcag
      301 tcaccggtca gtgcctttgc ggcggataaa ctgcatgtga cagacattac cagaggtgcg
      361 acttacccgg tactgataga cagcattgcg gtggaagtga acagcacaga cactgcggca
      421 tgataaaaaa accgccagcg acaggaatgg acgctggcgg tggtgatacc tatggagaaa
      481 aaataaagga acgatacttt cgtactctgg ttttttaatg aaaacagttc ttattgtcaa
      541 caataacgga aagaaattat gacatttctg aaccagttaa tgctgtactt ctgtacggtg
      601 gtctgtgtgc tgtatctcct ttcgggtgga taccgggcca tgcgtgacgt ctggcgcaga
      661 cagattgaca aaagggccgc tgagaaaatc agcgccagtc agtcagccgg aagcaaaccc
      721 gaagagccgc tcatttagcg gcaactttct taatcacatc tttcgacgag aaaatcccat
      781 gtcagaaatt acatccctgg tcactgctga agcagtgaag gaagtcctgc gctctgaaga
      841 agtccggagc gcactgaaac aaaaacttcg ccataacctg gaagcgcgtc ttgatgcaga
      901 ggttgatgcc attctggatg agctgcttgg tgtacaggca gagccaccga ctgaagcggg
      961 agataccacc gcagagagcg gtgaagttca gcctgaatca ccggtcgccg atgcgactga
     1021 acctcaaccc gaatcggtca tgatgctgta acggggagtc agggccatca gtagttaact
     1081 ggtggctttt ttattgttgt cagcttccgg ataacgggag acggggtatg taccagatgg
     1141 aaaaaatcac aacgggtgtg tcatacacca cgtcagcggt ggggacggga tactggttac
     1201 tgcagctgct ggacaaagtc tctccgtccc agtgggtggc aatcggtgtg ctggggagtc
     1261 tgctgtttgg cctgctgacg tatcttacta acctgtattt caaaatcaga gaggaccgtc
     1321 gtaaggcggc gcggggaaag taaagcgatg aagaaaaaat acgaactggt tgttaaaggg
     1381 ataaataatt acccggataa gattactgtt actgtggcac tggaaattgg tgggtatccg
     1441 tcactgttgt tgccagatgt g
//
LOCUS       DAZKHF020000505         1459 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2201S31241
            SAMN43267800-rid25106413.denovo.509, whole genome shotgun sequence.
ACCESSION   DAZKHF020000505 DAZKHF020000000
VERSION     DAZKHF020000505.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267800
            Sequence Read Archive: SRR30307263
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1459)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1459)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 1459)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350581.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350581.2
            Genome Coverage       :: 151x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:21:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,652
            CDSs (total)                      :: 5,557
            Genes (coding)                    :: 5,320
            CDSs (with protein)               :: 5,320
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 237
            CDSs (without protein)            :: 237
            Pseudo Genes (ambiguous residues) :: 0 of 237
            Pseudo Genes (frameshifted)       :: 102 of 237
            Pseudo Genes (incomplete)         :: 130 of 237
            Pseudo Genes (internal stop)      :: 52 of 237
            Pseudo Genes (multiple problems)  :: 41 of 237
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1459
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267800-rid25106413.denovo.509"
                     /serotype="O 26:H11"
                     /isolate="2201S31241"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-01"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            490..717
                     /gene="ykgL"
                     /locus_tag="ACHOSC_RS25280"
                     /old_locus_tag="pgaptmp_005056"
     CDS             490..717
                     /gene="ykgL"
                     /locus_tag="ACHOSC_RS25280"
                     /old_locus_tag="pgaptmp_005056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YkgL"
                     /protein_id="HGU4550800.1"
                     /translation="MQAAPQREETEWRVQSKRGLMPAYRGEAGQQVNIKIMEYSERNV
                     RQLASNEQEEYIPRKINVGVINTPTLIRSDY"
     gene            complement(752..892)
                     /gene="ykgO"
                     /locus_tag="ACHOSC_RS25285"
                     /old_locus_tag="pgaptmp_005057"
     CDS             complement(752..892)
                     /gene="ykgO"
                     /locus_tag="ACHOSC_RS25285"
                     /old_locus_tag="pgaptmp_005057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type B 50S ribosomal protein L36"
                     /protein_id="HGU4550801.1"
                     /translation="MKVLNSLRTAKERHPDCQIVKRKGRLYVICKSNPRFKAVQGRKK
                     KR"
     gene            complement(892..1155)
                     /gene="ykgM"
                     /locus_tag="ACHOSC_RS25290"
                     /old_locus_tag="pgaptmp_005058"
     CDS             complement(892..1155)
                     /gene="ykgM"
                     /locus_tag="ACHOSC_RS25290"
                     /old_locus_tag="pgaptmp_005058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type B 50S ribosomal protein L31"
                     /protein_id="HGU4550802.1"
                     /translation="MKPNIHPEYRTVVFHDTSVDEYFKIGSTIKTDREIELDGVTYPY
                     VTIDVSSKSHPFYTGKLRTVTSEGNVARFTQRFGRFVSTKKGA"
BASE COUNT          417 a          291 c          330 g          421 t
ORIGIN      
        1 tacttgcatt ttatattggt caggaacttt gccctgaagt gttaggaata gtcttaatgc
       61 gctgtgtgat ggactagtaa aaaaacaagt tttaagggta agggatagaa tataaaacta
      121 taatgagctg tcattgaaga tgatttgcca atcagtaaga aattttaatg gattaacatg
      181 ccgcacaaaa tggttatagg tgttcattac aataagtgat ggaaaattga acgggcgtat
      241 gtacgtcatc tgttcggtaa gcccccaaga gccagattaa gtgaagttaa acgaaggctg
      301 ccagctgtca caggaacgat ggcctctttg tcagggcact gagtgcagtt atctgtgcgc
      361 gttactctaa ctggcactcg aacgtcggct acgggttacc ggtggatcag actgttcctg
      421 tggcgtaaac gtttttatgt tagtaccaac agatttacca catttatcat cttaacagtt
      481 tgagtgaaaa tgcaggctgc gccgcaaagg gaggagactg agtggcgtgt tcagtctaaa
      541 agagggttga tgccagcata caggggcgaa gctggtcaac aagtcaacat taagataatg
      601 gaatattcgg agagaaacgt caggcagttg gcctcaaatg aacaggaaga atatatcccc
      661 aggaaaatta atgttggtgt tataaacaca ccaacattaa tccgatctga ttattaatca
      721 gacaaatatc gttaattcgt cgaattttga atcaacgttt tttcttacga ccctgaacgg
      781 ccttaaaacg tggattagat ttacaaatca catacaaccg tccttttcgc ttcacaatct
      841 gacagtctgg atggcgttct tttgcggtac gcagagagtt aagaactttc atcacgcccc
      901 ctttttcgtg ctaacaaaac gaccaaaacg ttgggtgaat cgtgcaacat ttccttctga
      961 tgtcactgtt ctcagcttcc ctgtatagaa cgggtgcgat ttagaagaga catcaattgt
     1021 cacgtacgga tacgttacgc catccagctc aatctcacgg tctgttttga tagtcgagcc
     1081 gattttaaag tactcatcaa cactggtgtc gtggaacacc acagtacgat actcaggatg
     1141 gatattgggc ttcatcattt ttacctgtta tgttataaca taaccgtaaa gatacatgct
     1201 catccggatc atttcaacac ccaatccctg tgcccatttg tggtatgtgg aatttcccca
     1261 acggggaagt ctggcattgt tagcagccgt tggcgacgct cccataaacg cctggatgag
     1321 tgattgatga tggttttgct gagagtatgg atttttcgag aatggaggtt atctccactt
     1381 tactggccga tgatttatcg cagtatgatt gattacctga cagcgtgtat tcctctccca
     1441 cggatataat aattactta
//
LOCUS       DAZKHF020000506         1455 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2201S31241
            SAMN43267800-rid25106413.denovo.510, whole genome shotgun sequence.
ACCESSION   DAZKHF020000506 DAZKHF020000000
VERSION     DAZKHF020000506.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267800
            Sequence Read Archive: SRR30307263
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1455)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1455)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 1455)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350581.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350581.2
            Genome Coverage       :: 151x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:21:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,652
            CDSs (total)                      :: 5,557
            Genes (coding)                    :: 5,320
            CDSs (with protein)               :: 5,320
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 237
            CDSs (without protein)            :: 237
            Pseudo Genes (ambiguous residues) :: 0 of 237
            Pseudo Genes (frameshifted)       :: 102 of 237
            Pseudo Genes (incomplete)         :: 130 of 237
            Pseudo Genes (internal stop)      :: 52 of 237
            Pseudo Genes (multiple problems)  :: 41 of 237
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1455
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267800-rid25106413.denovo.510"
                     /serotype="O 26:H11"
                     /isolate="2201S31241"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-01"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            complement(<1..1178)
                     /locus_tag="ACHOSC_RS25295"
                     /old_locus_tag="pgaptmp_005059"
     CDS             complement(<1..1178)
                     /locus_tag="ACHOSC_RS25295"
                     /old_locus_tag="pgaptmp_005059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HGU4550803.1"
                     /translation="MWNLLRRTRKNQKSGRDVREAGWTSLFQAVAEPFSGAWQQGVKA
                     DPEAVLSFHAVFACISLISQDIAKMRLRLMQTDAHGIRRETRRGDIARLCRRPNAQQN
                     RIQFFELWLNAKLRHGNTVVLKIRNARGQIKELRILDWSRVEPLVADDGEVFYRITPD
                     RNCGITEAVTVPAREVIHDRFNCFFHPLIGLPPVYVAGLAATQGHHIQENSTSFFRNG
                     GRPSGVIEIPGSITEENAKKLKSNWDSGYTGENAGKTAILSNGAKYNPTTFSPVDAQT
                     VEQLKMTAEIVCSVFRVPAYKIGVGQPPSSDNVEALEQQYYSQCLQTLIESIELLLDE
                     ALETGENESTEFDVTTLLRMDSERRMKTLGDAVKNTLLTPNEARKRENLPPLAGGDAL
                     "
     gene            complement(1124..1345)
                     /locus_tag="ACHOSC_RS25300"
                     /old_locus_tag="pgaptmp_005060"
     CDS             complement(1124..1345)
                     /locus_tag="ACHOSC_RS25300"
                     /old_locus_tag="pgaptmp_005060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4550804.1"
                     /translation="MAKIRYLQGTHDARAGDIRDVAQPCAEVLVRLGKAEYITVRRPA
                     GQKKKRDAEHGECGTFYGEPEKTRNQDVT"
BASE COUNT          283 a          479 c          358 g          335 t
ORIGIN      
        1 tacagtgcat caccgccggc caggggcggc aggttctccc gtttacgggc ctcattgggc
       61 gtgagaagcg tatttttcac cgcatccccc agcgttttca tgcgccgctc actgtccatt
      121 ctcagcagcg tggtgacatc aaattctgta ctctcgtttt cccccgtttc cagcgcctca
      181 tccagtaaca gttcaatgga ctcaatcagc gtctgcaggc actgggaata atactgctgc
      241 tccagcgcct ccacgttgtc actggaaggc ggttgtccca cgccaatctt gtaggccggg
      301 acacggaaca ccgaacagac aatttcagcg gtcatcttca gttgttccac cgtctgcgca
      361 tccacaggtg aaaacgtcgt ggggttgtat tttgccccgt tgctcagaat ggccgttttc
      421 cccgcatttt cgcctgtata cccgctgtcc cagttgctct tcagtttttt cgcattttct
      481 tccgtaatac tgccggggat ctcaatcacc ccggacggcc tgccgccatt tctgaaaaaa
      541 gacgtcgaat tttcctgaat atgatgcccc tgcgtggccg ccagcccggc gacatacacc
      601 ggcggcaatc ctataagcgg atgaaaaaaa cagttaaacc ggtcgtggat cacttcccgg
      661 gcaggcaccg tcaccgcctc cgtgatcccg cagttccggt ccggcgtgat gcggtagaac
      721 acctcgccgt catccgccac cagaggttca acccggctcc agtccagaat acgcagttct
      781 ttgatctgcc cccgggcatt acggattttc agcaccaccg tattgccatg acgcagtttg
      841 gcgttcagcc acagttcaaa aaactggatg cggttctgct gggcgttggg acgacgacag
      901 aggcgggcaa tatccccccg gcgcgtttcc ctgcgtatcc catgcgcatc cgtctgcata
      961 agacgcagcc gcattttggc gatatcctgg gatatcagcg aaatacatgc aaacaccgca
     1021 tgaaaggaga ggacggcttc aggatcggct ttcacgccct gctgccaggc gccggaaaag
     1081 ggctcagcca ccgcctgaaa caggctggtc cagcccgcct ctcttacgtc acgtcctgat
     1141 ttctggtttt ttcgggttcg ccgtaaaagg ttccacattc gccatgctcc gcatcacgtt
     1201 tctttttctg acctgccgga cgtcgcaccg tgatgtactc cgcctttccc aggcgaacca
     1261 gcacctccgc acacggctgt gccacatcac ggatatcccc ggcccgggca tcatgcgtgc
     1321 cctgcagata tcggatcttt gccataacct gttacgggag gcgcacgcct cccgtcctcc
     1381 ttatcagact cagccgccgg acgcactgcc gtagttcact ccggtgatca ccgccaccgc
     1441 cacaccgccg tcatc
//
LOCUS       DAZKHF020000507         1435 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2201S31241
            SAMN43267800-rid25106413.denovo.511, whole genome shotgun sequence.
ACCESSION   DAZKHF020000507 DAZKHF020000000
VERSION     DAZKHF020000507.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267800
            Sequence Read Archive: SRR30307263
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1435)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1435)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 1435)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350581.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350581.2
            Genome Coverage       :: 151x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:21:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,652
            CDSs (total)                      :: 5,557
            Genes (coding)                    :: 5,320
            CDSs (with protein)               :: 5,320
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 237
            CDSs (without protein)            :: 237
            Pseudo Genes (ambiguous residues) :: 0 of 237
            Pseudo Genes (frameshifted)       :: 102 of 237
            Pseudo Genes (incomplete)         :: 130 of 237
            Pseudo Genes (internal stop)      :: 52 of 237
            Pseudo Genes (multiple problems)  :: 41 of 237
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1435
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267800-rid25106413.denovo.511"
                     /serotype="O 26:H11"
                     /isolate="2201S31241"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-01"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            complement(18..329)
                     /locus_tag="ACHOSC_RS25305"
                     /old_locus_tag="pgaptmp_005061"
     CDS             complement(18..329)
                     /locus_tag="ACHOSC_RS25305"
                     /old_locus_tag="pgaptmp_005061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001314746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose/cellobiose transporter subunit IIA"
                     /protein_id="HGU4550805.1"
                     /translation="MFADEELVMELLVNAGQARSDAMEAIRCAGQKDWQGATQLMASS
                     ESACLQAHKIQTALISQDEGCGKIKVNLILIHAQDHLMNAILCQDLAREIISLRKELH
                     A"
     gene            complement(357..>1435)
                     /locus_tag="ACHOSC_RS25310"
                     /old_locus_tag="pgaptmp_005062"
     CDS             complement(357..>1435)
                     /locus_tag="ACHOSC_RS25310"
                     /old_locus_tag="pgaptmp_005062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001736301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016773 - phosphotransferase activity,
                     alcohol group as acceptor [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HGU4550806.1"
                     /translation="TPVMNGSVNIMSIMIAFLVARNMAISYEQDDLLCGLTAIGAFFI
                     VYTPYQMIDGQAFLTTKYLGAQGLFVAVIVALITSEIFCRLARNPKITITMPAAVPPA
                     VARSFKVLLPIFFVMVFFSALNYCLTLISPAGLNDLIYTLIQTPLKHMGTNIFAVIIL
                     GAVGNFLWVLGIHGPNTTSAIRETVFSEANLENLSWAAQHGTTWGAPYPITWTSINDA
                     FANCGGSGMTLGLLLAIFIASKRAEYRDLAKMSFIPGIFNINEPIMFDLPIVLNPIMM
                     VPFIMVPIVNCAIGYFFVSMEIIPPVAYAVPWTTPGPLIAFLGTGGNWLALLVGFLCL
                     GVATMIYLPFVIAANKVNNMTTNG"
BASE COUNT          447 a          330 c          302 g          356 t
ORIGIN      
        1 cgcactggag gaaaaaatca ggcatggagt tcttttctta atgaaataat ttccctggct
       61 aaatcctggc ataatattgc attcatcaaa tgatcctgtg catgaatcag aattaaatta
      121 acttttattt tgccacagcc ttcatcctga ctaattaatg cggtctgaat cttatgtgcc
      181 tgaagacagg cagattcaga actggccatt agctgagttg cgccctgcca gtctttttgc
      241 ccggcacaac gtatggcttc catcgcgtca gaacgtgcct gccccgcgtt gaccagtaac
      301 tccataacaa gttcttcatc tgcaaacatc acaatctcct tcctgggtaa atggaattat
      361 ccgttagttg tcatgttatt gactttgttg gcggcaataa caaaaggtaa atagatcatt
      421 gtcgccacac ctaaacataa aaaaccaacc agtaaagcca gccagttccc cccggttccg
      481 aggaaagcaa ttaaaggtcc gggcgtagtc cagggcacgg cataagcaac cggtggaata
      541 atttccatcg aaacaaagaa gtaaccaatg gcacagttaa caatgggaac cataataaac
      601 ggcaccatca tgatggggtt aagtacaata ggaaggtcaa acattatcgg ttcattgata
      661 ttgaaaatac cggggataaa tgacattttt gccagatcac ggtattccgc acgcttagaa
      721 gcgataaaaa tagccaacaa taaccccaac gtcatacctg aaccgccgca gttggcgaat
      781 gcatcattaa tagaagtcca ggtaatcgga tatggcgcgc cccaggtagt gccatgttga
      841 gcggcccagg agagattctc cagattagcc tcagaaaaaa cggtttctcg aattgccgat
      901 gtggtattag gtccgtggat ccccagcacc cacaggaaat tacccacagc ccccaggata
      961 attaccgcaa agatattcgt tcccatatgt ttgagcggcg tctggattaa tgtgtaaata
     1021 agatcgttta gtcctgccgg ggatatcagt gtcaggcaat aattaagtgc ggaaaagaac
     1081 accatgacaa aaaatattgg caataaaact ttaaatgaac gcgctaccgc aggcggtaca
     1141 gctgccggca tcgttatggt gatttttggg tttcgagcta agcgacaaaa tatttcactg
     1201 gtgatcaatg caacgataac agcaacaaac aacccctgcg cgccgagata tttggtcgtc
     1261 aggaatgctt gcccatctat catctgatat ggcgtataaa caataaaaaa tgctcctatt
     1321 gccgttaatc cgcataaaag atcatcttgc tcatagctaa tcgccatatt cctggcgacc
     1381 aggaaagcaa tcataattga catgatattt acagaaccat tcattaccgg agtaa
//
LOCUS       DAZKHF020000508         1414 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2201S31241
            SAMN43267800-rid25106413.denovo.512, whole genome shotgun sequence.
ACCESSION   DAZKHF020000508 DAZKHF020000000
VERSION     DAZKHF020000508.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267800
            Sequence Read Archive: SRR30307263
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1414)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1414)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 1414)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350581.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350581.2
            Genome Coverage       :: 151x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:21:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,652
            CDSs (total)                      :: 5,557
            Genes (coding)                    :: 5,320
            CDSs (with protein)               :: 5,320
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 237
            CDSs (without protein)            :: 237
            Pseudo Genes (ambiguous residues) :: 0 of 237
            Pseudo Genes (frameshifted)       :: 102 of 237
            Pseudo Genes (incomplete)         :: 130 of 237
            Pseudo Genes (internal stop)      :: 52 of 237
            Pseudo Genes (multiple problems)  :: 41 of 237
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1414
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267800-rid25106413.denovo.512"
                     /serotype="O 26:H11"
                     /isolate="2201S31241"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-01"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            <1..241
                     /locus_tag="ACHOSC_RS25315"
                     /old_locus_tag="pgaptmp_005063"
     CDS             <1..241
                     /locus_tag="ACHOSC_RS25315"
                     /old_locus_tag="pgaptmp_005063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="lyase"
                     /protein_id="HGU4550807.1"
                     /translation="QDKNEEVRIEAIIGLSYRKDKRVLSVLCDELKKNTVYDDIIEAA
                     GELGDKTLLPVLDTMLYKFDDNEIITSAIDKLKRS"
     gene            269..1227
                     /locus_tag="ACHOSC_RS25320"
                     /old_locus_tag="pgaptmp_005064"
                     /pseudo
     CDS             269..1227
                     /locus_tag="ACHOSC_RS25320"
                     /old_locus_tag="pgaptmp_005064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312497.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="RHS repeat-associated core domain-containing
                     protein"
     gene            1239..>1414
                     /locus_tag="ACHOSC_RS25325"
                     /old_locus_tag="pgaptmp_005065"
     CDS             1239..>1414
                     /locus_tag="ACHOSC_RS25325"
                     /old_locus_tag="pgaptmp_005065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sel1 repeat family protein"
                     /protein_id="HGU4550808.1"
                     /translation="MKACLLLFFYFSLIGQLHGADVKIKENESVMGSTAMTYDLSEEK
                     LMKLKYKSQHGDSEA"
BASE COUNT          430 a          269 c          353 g          362 t
ORIGIN      
        1 tcaggataaa aatgaagaag tccgtattga agcaataatc ggactttcct acagaaaaga
       61 taaaagggtt ttatctgttt tatgcgatga gttaaaaaaa aatactgttt atgatgatat
      121 cattgaagct gcgggtgaat taggtgataa aacgctactt cctgttttag atactatgtt
      181 gtacaagttt gatgacaatg aaattataac ttccgctatt gataagctga agcgttcata
      241 gcgtgagttg cctatgcaca gtgggggatt cccgccggca cggtgcagat gctcgaccgg
      301 ctggaaagtg aaatcctggc tgaccgggtg agtgaggaaa gccgccgctg gctggcatcg
      361 tgcggcctga ccgtggagca gatgaaaaac cagatggacc cggtgtacac gccggcgcga
      421 aaaatccacc tgtaccactg cgaccatcgc ggcctgccgc tggcgctcat cagcacggaa
      481 ggggcaacag cgtggtgcgc agaatatgat gaatggggca acctgctgag tgatgagaac
      541 ccgcatcatc tgcagcagct cattcgcctg ccgggtcagc agtatgatga ggagtccggc
      601 ctgtattaca accgccaccg ctattatgac ccgctgcagg ggcggtatat cactcaggat
      661 ccgattgggc tgaagggggg atggaatttt tatcagtatc cgttgaatcc ggtcataaat
      721 gtagatccgc aaggtttggt tgatataaat ttataccccg aaagtgatct tatccattct
      781 gtagctgatg agattaatat cccaggcgtt ttcacaatcg gggggcatgg tacccccaca
      841 tctattgaat ccgcaacgcg cagtatcatg acagctaaag atctagcata tctaattaaa
      901 tttgatggga attataaaga tgggatgaca gtttggttat tttcttgtaa tacaggtaaa
      961 ggacaaaatt catttgctag ccaattagct aaagagttac atacaaatgt aataggacct
     1021 gacacgctat ggacgtggtg ggggcgagga actaatggta agttaaaaat ggatacagtg
     1081 ctaacagcac caacgaacct taattcaaat aaggatctaa tggctataac aacaaaagac
     1141 cttggtaatt ggataacata tgggccatct gggcacccca tttctaatat gcaaggtacg
     1201 ccagaaaaac ccagtgatat aagataggtt gtagatgtat gaaagcatgc ttgttactat
     1261 ttttttattt ttctcttatt ggtcaattgc atggtgctga tgtgaaaata aaagaaaacg
     1321 aaagtgtgat gggatctaca gcaatgacct atgatttaag tgaagaaaag ctgatgaaac
     1381 tgaagtataa atcacaacat ggtgattcag aggc
//
LOCUS       DAZKHF020000509         1411 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2201S31241
            SAMN43267800-rid25106413.denovo.513, whole genome shotgun sequence.
ACCESSION   DAZKHF020000509 DAZKHF020000000
VERSION     DAZKHF020000509.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267800
            Sequence Read Archive: SRR30307263
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1411)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1411)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 1411)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350581.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350581.2
            Genome Coverage       :: 151x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:21:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,652
            CDSs (total)                      :: 5,557
            Genes (coding)                    :: 5,320
            CDSs (with protein)               :: 5,320
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 237
            CDSs (without protein)            :: 237
            Pseudo Genes (ambiguous residues) :: 0 of 237
            Pseudo Genes (frameshifted)       :: 102 of 237
            Pseudo Genes (incomplete)         :: 130 of 237
            Pseudo Genes (internal stop)      :: 52 of 237
            Pseudo Genes (multiple problems)  :: 41 of 237
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1411
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267800-rid25106413.denovo.513"
                     /serotype="O 26:H11"
                     /isolate="2201S31241"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-01"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            <1..889
                     /locus_tag="ACHOSC_RS25330"
                     /old_locus_tag="pgaptmp_005066"
     CDS             <1..889
                     /locus_tag="ACHOSC_RS25330"
                     /old_locus_tag="pgaptmp_005066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF1983 domain-containing protein"
                     /protein_id="HGU4550809.1"
                     /translation="SRLDEFSKEWKDANDKWNAMWGVKIEQTKDGKHYVAGIGLSMED
                     TEEGKLSQFLVAANRIAFIDPANGNETPMFVAQGNQIFMNDVFLKRLTAPTITSGGSP
                     PVFSLTSDGKLTAKNADISGRVNANAGTLNNVTVNENCTIKGMLEATQVRGDFVKAVS
                     KSFPKQAGTWGNTETPNGTVTVTISDDHNFDRQIIIPPIIFNGIAYSDPGSGNNPGGT
                     RYTGYGFEVRKNGVLIASRETKGAIPGSYSAVIDMPSGGGSVTLEFKIFQKGNQGAGN
                     ITDCTVIVTKKAASGISIR"
     gene            960..>1411
                     /locus_tag="ACHOSC_RS25335"
                     /old_locus_tag="pgaptmp_005067"
     CDS             960..>1411
                     /locus_tag="ACHOSC_RS25335"
                     /old_locus_tag="pgaptmp_005067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044384 - host outer membrane [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Ail/Lom family outer membrane beta-barrel
                     protein"
                     /protein_id="HGU4550810.1"
                     /translation="MRKLYAAILSAAICLAVSGASAWASEHQSTLSAGYLHASTNVPG
                     SDDLNGINVKYRYEFTDTLGLVTSFSYAGDKNRQLTRYSDIRWHEDSVRNRWFSVMAG
                     PSVRVNEWFSAYAMAGVSYSRVSTFSGDYLRVTDNKGKTHDVLTGSDDG"
BASE COUNT          375 a          311 c          400 g          325 t
ORIGIN      
        1 cagcagactg gatgagtttt cgaaagagtg gaaggacgct aacgataaat ggaatgccat
       61 gtggggcgtc aaaattgagc agaccaaaga cggcaaacat tatgtcgcgg gtattggcct
      121 cagcatggag gacacggagg aaggcaagct gagccagttt ctggttgccg ctaaccgtat
      181 cgcgtttatt gacccggcaa acgggaatga aacgccgatg tttgtggcgc agggtaacca
      241 gatattcatg aacgacgtgt tcctgaagcg cctgacagcc cccaccatta ccagcggtgg
      301 cagtcctccg gtattttctc tgacatcaga cggaaagctg accgctaaaa atgcggatat
      361 cagtggcaga gtgaatgcga acgccgggac gctcaacaat gtcacggtaa atgaaaactg
      421 tacgattaag ggcatgctgg aggcgactca ggtcagaggt gacttcgtta aagctgtatc
      481 caaatcattc ccgaaacagg ctggtacgtg gggtaatacg gaaacaccaa acgggacggt
      541 tacagtcacc atcagcgatg atcataactt tgaccgtcaa atcattattc cgcccattat
      601 ctttaacgga atagcgtata gcgatccggg aagtggtaat aacccgggag gtacaagata
      661 cacgggttat ggttttgaag ttcgcaaaaa cggtgtatta atcgcatcca gagaaactaa
      721 aggggccatt cccggtagtt acagtgcagt tattgatatg cctagtggtg gtggtagcgt
      781 cactctggag tttaagattt tccagaaagg caatcagggg gcagggaata tcaccgactg
      841 tacggtgatt gtgaccaaaa aagcggcttc cggtatcagt attcgttgaa atatttataa
      901 ccccaataaa gggcgtcagg aatgacgcct tttttattgc agaaaagcga gaggtaatta
      961 tgcgtaaact ttatgcagcc attttgtccg cagctatttg tctggccgta tccggtgcgt
     1021 ctgcatgggc atctgaacat cagtccacgc tgagcgcggg gtatcttcat gcctcgacga
     1081 acgttcccgg cagcgatgat ctgaacggga ttaacgtgaa ataccgttat gagtttacgg
     1141 acacactggg gctggtgacg tcattcagct atgcaggaga caagaatcgc cagcttaccc
     1201 gttacagcga tatccgctgg catgaagatt ccgtgcgtaa ccgctggttc agcgtgatgg
     1261 cggggccgtc tgtgcgcgtg aatgaatggt tcagcgcgta tgcgatggcg ggtgtgtctt
     1321 acagccgtgt gtcgactttc tccggggatt atctccgcgt aactgacaac aaggggaaaa
     1381 cgcacgacgt gctgaccgga agtgatgacg g
//
LOCUS       DAZKHF020000510         1401 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2201S31241
            SAMN43267800-rid25106413.denovo.514, whole genome shotgun sequence.
ACCESSION   DAZKHF020000510 DAZKHF020000000
VERSION     DAZKHF020000510.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267800
            Sequence Read Archive: SRR30307263
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1401)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1401)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 1401)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350581.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350581.2
            Genome Coverage       :: 151x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:21:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,652
            CDSs (total)                      :: 5,557
            Genes (coding)                    :: 5,320
            CDSs (with protein)               :: 5,320
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 237
            CDSs (without protein)            :: 237
            Pseudo Genes (ambiguous residues) :: 0 of 237
            Pseudo Genes (frameshifted)       :: 102 of 237
            Pseudo Genes (incomplete)         :: 130 of 237
            Pseudo Genes (internal stop)      :: 52 of 237
            Pseudo Genes (multiple problems)  :: 41 of 237
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1401
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267800-rid25106413.denovo.514"
                     /serotype="O 26:H11"
                     /isolate="2201S31241"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-01"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            complement(<1..>1401)
                     /locus_tag="ACHOSC_RS25340"
                     /old_locus_tag="pgaptmp_005068"
     CDS             complement(<1..>1401)
                     /locus_tag="ACHOSC_RS25340"
                     /old_locus_tag="pgaptmp_005068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1983 domain-containing protein"
                     /protein_id="HGU4550811.1"
                     /translation="VAITAHPVPDRIQVSTLPDGVETYGVWGLSLPSLRRRLFRCVSI
                     RENTDGTFAITAVQHVPEKEAIVDNGAHFDGDQSGTVNGVTPPAVQHLTAEVTADSGE
                     YQVLARWDTPKVVKGVSFLLRLTVAADDGSERLVSTARTTETTYRFTQLAPGNYRLTV
                     RAVNAWGQQGDPASVSFRIAAPAAPSRIELTPGYFQITATPHLAVYDPTVQFEFWFSE
                     KRIADIRQVETTARYLGTALYWIAASINIKPGHDYYFYIRSVNTVGKSAFVEAVGQPS
                     DDASGYLDFFKGEIGKTHLAQELWTQIDNGQLAPDLTEIRTSITDVSNEITQTVNKKL
                     EDQSAAIQQIQKVQVDTNNNLNSMWAVKLQQMQDGRLYIAGIGAGIENTPDGMQSQVL
                     LAADRIAMINPANGNTKPMFVGQGDQIFMNEVFLKYLTAPTITSGGNPPAFSLTPDGR
                     LTAKNADISGSVNANSG"
BASE COUNT          278 a          423 c          362 g          338 t
ORIGIN      
        1 tcctgagttc gcattcacac tgccactgat atccgcattt ttcgccgtca gtcgcccgtc
       61 tggtgtcagg gaaaatgccg gaggattacc gccgctggta atggtgggag ccgtcagata
      121 tttcaggaac acttcattca tgaatatctg atcgccctga ccaacaaaca tcggctttgt
      181 gttgccattc gcaggattaa tcatcgcaat cctgtctgct gccagcagca cctgactctg
      241 catgccgtcg ggggtgttct caataccggc accgataccc gcaatataaa ggcgtccgtc
      301 ctgcatctgc tgcagcttca ctgcccacat gctgttcagg ttattatttg tatcaacctg
      361 aaccttctgt atctgctgga tcgctgcact ctggtcttcc agtttcttat tgacggtctg
      421 tgttatttca ttgctgacat ccgttatgga cgtcctgatt tcagtcaggt caggcgcaag
      481 ctgaccgtta tcaatctgcg tccacagctc ctgagccaga tgggttttcc ctatctcgcc
      541 tttgaaaaaa tccagatagc ctgatgcatc atcactcggc tgaccgacag cctctacaaa
      601 tgccgatttg ccaacggtgt tcacactgcg gatataaaag taataatcat ggcccggttt
      661 gatattgata ctggcagcta tccagtacag cgccgtgcca agatagcggg ctgtggtttc
      721 aacctgcctg atatcagcaa tccgcttttc cgagaaccag aactcaaact gtaccgtcgg
      781 atcataaacc gcaagatgcg gcgtggcggt tatctgaaaa tagcccggtg tcagctcaat
      841 ccgagacggc gctgccggtg cggcaatccg gaacgatacc gatgccggat cgccctgctg
      901 tccccacgca tttaccgccc ggactgtcaa cctgtagttc cccggcgcca gttgcgtgaa
      961 gcggtatgtg gtttctgtcg tcctggccgt gctgaccagc cgctcactgc cgtcatccgc
     1021 tgccacggtc aggcgaagca ggaagctcac gcccttcacc accttcggcg tgtcccagcg
     1081 cgccagcacc tgatattccc cgctgtctgc ggtgacttcg gcggtcaggt gctgcaccgc
     1141 tggcggcgtg acaccattca ccgtgccgct ctggtcgccg tcaaagtgcg ccccgttatc
     1201 cacgatggct tctttttccg gcacgtgctg caccgccgtg atggcaaagg tgccgtccgt
     1261 gttttcccgg atggagacac agcggaacag gcgacgacgc agtgacggca gggagagtcc
     1321 ccatacaccg tatgtctcca caccatcagg cagggtgctg acctgtatcc ggtccggcac
     1381 ggggtgtgca gtgatggcca c
//
LOCUS       DAZKHF020000511         1391 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2201S31241
            SAMN43267800-rid25106413.denovo.515, whole genome shotgun sequence.
ACCESSION   DAZKHF020000511 DAZKHF020000000
VERSION     DAZKHF020000511.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267800
            Sequence Read Archive: SRR30307263
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1391)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1391)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 1391)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350581.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350581.2
            Genome Coverage       :: 151x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:21:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,652
            CDSs (total)                      :: 5,557
            Genes (coding)                    :: 5,320
            CDSs (with protein)               :: 5,320
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 237
            CDSs (without protein)            :: 237
            Pseudo Genes (ambiguous residues) :: 0 of 237
            Pseudo Genes (frameshifted)       :: 102 of 237
            Pseudo Genes (incomplete)         :: 130 of 237
            Pseudo Genes (internal stop)      :: 52 of 237
            Pseudo Genes (multiple problems)  :: 41 of 237
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1391
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267800-rid25106413.denovo.515"
                     /serotype="O 26:H11"
                     /isolate="2201S31241"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-01"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            complement(<1..184)
                     /locus_tag="ACHOSC_RS25345"
                     /old_locus_tag="pgaptmp_005069"
     CDS             complement(<1..184)
                     /locus_tag="ACHOSC_RS25345"
                     /old_locus_tag="pgaptmp_005069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysozyme"
                     /protein_id="HGU4550812.1"
                     /translation="MSPSLRKAVAAAIGGGAVAIASVLITGPSGDDGLEGVSYIPYED
                     IVGVWTVCHGHTGKDII"
     gene            complement(184..399)
                     /locus_tag="ACHOSC_RS25350"
                     /old_locus_tag="pgaptmp_005070"
     CDS             complement(184..399)
                     /locus_tag="ACHOSC_RS25350"
                     /old_locus_tag="pgaptmp_005070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0020002 - host cell plasma membrane
                     [Evidence IEA];
                     GO_function: GO:0042802 - identical protein binding
                     [Evidence IEA];
                     GO_function: GO:0140911 - pore-forming activity [Evidence
                     IEA];
                     GO_process: GO:0019835 - cytolysis [Evidence IEA];
                     GO_process: GO:0044659 - viral release from host cell by
                     cytolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class II holin family protein"
                     /protein_id="HGU4550813.1"
                     /translation="MYQMEKITTGVSYTTSAVGTGYWLLQLLDKVSPSQWVAIGVLGS
                     LVFGLLTYLTNLYFKIKEDKRKAARGE"
     gene            542..940
                     /locus_tag="ACHOSC_RS25355"
                     /old_locus_tag="pgaptmp_005071"
     CDS             542..940
                     /locus_tag="ACHOSC_RS25355"
                     /old_locus_tag="pgaptmp_005071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_303550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4550814.1"
                     /translation="MLHIISPRGISPRQRRLFYPFSSALALFYYAAAFTSGTMLSIST
                     QYVVLLVTNIITINRHRISPVYRNRVKKPEPESNHRMTICQWQVIKKRPRHAQPELTR
                     KMIEGNNISDVRMAPPAKFCNDQTELATCP"
     gene            complement(1021..1179)
                     /locus_tag="ACHOSC_RS25360"
                     /old_locus_tag="pgaptmp_005072"
     CDS             complement(1021..1179)
                     /locus_tag="ACHOSC_RS25360"
                     /old_locus_tag="pgaptmp_005072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000499449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3927 family protein"
                     /protein_id="HGU4550815.1"
                     /translation="MFYKLCLLAVVILLLVMVMMDFTSRIMLVLTDGALVCGIVVLLW
                     PMMKEQNE"
     gene            complement(1265..>1391)
                     /locus_tag="ACHOSC_RS25365"
                     /old_locus_tag="pgaptmp_005073"
     CDS             complement(1265..>1391)
                     /locus_tag="ACHOSC_RS25365"
                     /old_locus_tag="pgaptmp_005073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308843.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4550816.1"
                     /translation="LARYDNCLVLSTSFNAGSKRFVIYQSAQDKNLQVDIVRTRI"
BASE COUNT          425 a          357 c          251 g          358 t
ORIGIN      
        1 gaatgatgtc ttttccggtg tgtccgtgac atacagtcca tacgccaacg atatcttcgt
       61 atggtatgta gctgacacct tccaggccat cgtcaccact cggaccagtg atgagcacag
      121 acgctatggc aacagcccca ccaccaatag cagcagcaac agccttgcgt aatgatggcg
      181 acattattca cctctcgcag ccttacgctt atcttcttta atcttgaaat aaaggtttgt
      241 cagatacgtc agcaagccaa acaccaggct acccaatacg cctattgcca cccactggga
      301 tggggagact ttgtccagca actgcagtag ccagtatccc gtccccaccg ctgacgtggt
      361 gtatgacaca cctgttgtta ttttttccat ctggtacata ccccgtctcc cgttatccgg
      421 aagctgacaa caataaaaag ccaccagtta attcctgatg gccctgatgc ataaacgtca
      481 taatacctga ctgttatgat tgacaataat gataatgttt atatagaaag gttcccgatg
      541 tgtgttacat atcatttctc cacggggaat atccccacgc cagcgcagac tcttttaccc
      601 gttctcttct gcgctggctc ttttttatta tgctgctgca tttacctctg gcaccatgct
      661 ttctatctca acacaatacg tggtacttct tgtaaccaat atcataacga ttaatcgaca
      721 tagaatttct cccgtgtaca ggaacagagt taaaaagccg gaaccggaat caaatcacag
      781 gatgaccatc tgccagtggc aggtcataaa aaaaaggccg cgccatgcgc agccagaact
      841 cacaaggaaa atgatagaag gaaataacat tagtgatgta cgcatggcgc ctcccgctaa
      901 gttctgcaat gatcaaacag aactcgctac gtgcccttaa aactcgatca tttagcccct
      961 ccaaggagga ttcaccatgc ggttgatttt ttaataaaca gtaaacaaaa aagtcaagaa
     1021 ttattcattc tgttctttca tcatcggcca cagcaatacc acaatgccgc agaccagagc
     1081 gccatcagtc agtaccaaca ttatcctgct ggtgaaatcc atcatcacca tcactaaaag
     1141 caggatcaca acagcaagca gacacagttt ataaaacaat gttcagaaaa cgcattcagc
     1201 atgcctaagg ttctattcct acgaatagcc aacttgcaac ttaaaatatt atttatgcag
     1261 ccaattaaat tctggtcctt acaatatcaa cctgaagatt cttatcttgt gctgattgat
     1321 aaatgacaaa ccttttacta cctgcattga aagaagtaga caaaaccaga caattatcat
     1381 aacgagcaag a
//
LOCUS       DAZKHF020000512         1388 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2201S31241
            SAMN43267800-rid25106413.denovo.516, whole genome shotgun sequence.
ACCESSION   DAZKHF020000512 DAZKHF020000000
VERSION     DAZKHF020000512.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267800
            Sequence Read Archive: SRR30307263
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1388)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1388)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 1388)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350581.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350581.2
            Genome Coverage       :: 151x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:21:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,652
            CDSs (total)                      :: 5,557
            Genes (coding)                    :: 5,320
            CDSs (with protein)               :: 5,320
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 237
            CDSs (without protein)            :: 237
            Pseudo Genes (ambiguous residues) :: 0 of 237
            Pseudo Genes (frameshifted)       :: 102 of 237
            Pseudo Genes (incomplete)         :: 130 of 237
            Pseudo Genes (internal stop)      :: 52 of 237
            Pseudo Genes (multiple problems)  :: 41 of 237
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1388
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267800-rid25106413.denovo.516"
                     /serotype="O 26:H11"
                     /isolate="2201S31241"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-01"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            107..721
                     /gene="evgA"
                     /locus_tag="ACHOSC_RS25370"
                     /old_locus_tag="pgaptmp_005074"
     CDS             107..721
                     /gene="evgA"
                     /locus_tag="ACHOSC_RS25370"
                     /old_locus_tag="pgaptmp_005074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid-sensing system DNA-binding response
                     regulator EvgA"
                     /protein_id="HGU4550817.1"
                     /translation="MNAIIIDDHPLAIAAIRNLLIKNDIEILAELTEGGSAVQRVETL
                     KPDIVIIDVDIPGVNGIQVLETLRKRQYSGIIIIVSAKNDHFYGKHCADAGANGFVSK
                     KEGMNNIIAAIEAAKNGYCYFPFSLNRFVGSLTSDQQKLDSLSKQEISVMRYILDGKD
                     NNDIAEKMFISNKTVSTYKSRLMEKLECKSLMDLYTFAQRNKIG"
     gene            726..>1388
                     /locus_tag="ACHOSC_RS25375"
                     /old_locus_tag="pgaptmp_005075"
     CDS             726..>1388
                     /locus_tag="ACHOSC_RS25375"
                     /old_locus_tag="pgaptmp_005075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HGU4550818.1"
                     /translation="MKFLPYFFLLCCGLWSTISFADEDYIEYRGISSNNRVTLDPVRL
                     SNKELRWLASKKNLVIAVHKSQTATLLHTDSQQQVRGINADYLNLLKRALNIKLTLRE
                     YADHQKAMDALAEGEVDIVLSHLVTSPPLNSDIAATKPLIITFPALVTTLHDSMRPLT
                     SPKPVNIARVANYPPDEVIHQSFPKATIISFTNLYQALASVSAGQNDYFIGSNIITSS
                     MIS"
BASE COUNT          437 a          283 c          275 g          393 t
ORIGIN      
        1 aaaggaagct cagattttct tatttttatt gagaaaatga gatgacgcct tatgtctgta
       61 ttactacagg gagaagggag atgcttcatt gcaaagggaa taatctatga acgcaataat
      121 tattgatgac catcctcttg ctatcgcagc aattcgtaat ttattgatca aaaacgatat
      181 tgaaatctta gcggagttga ctgaaggggg aagcgccgtt cagcgggtgg aaacacttaa
      241 gcctgatatc gtcatcattg atgtcgatat ccccggagtt aacggtatcc aggtgttaga
      301 aacgctgagg aagcgccaat atagcggaat tattattatt gtctccgcta aaaatgacca
      361 tttttacggg aaacattgtg ctgatgctgg cgctaatgga ttcgtgagta aaaaagaagg
      421 catgaacaat atcattgcgg ctattgaagc tgcaaaaaat ggctactgct atttcccctt
      481 ctctctcaac cggtttgttg gaagtttaac gtccgaccag caaaaactcg actccttatc
      541 gaaacaagaa attagtgtca tgcggtatat tcttgatggc aaggataata atgacattgc
      601 tgaaaaaatg ttcatcagca acaaaactgt cagcacttat aaaagtcgcc tgatggaaaa
      661 attagaatgt aaatcactga tggatcttta cacattcgcg caacgtaaca aaatcggcta
      721 atcacatgaa gtttttaccc tatttttttc ttctctgttg tggtctttgg tcgaccataa
      781 gtttcgcaga cgaagattac atcgaatatc gtggcatcag tagtaacaac cgtgtcacac
      841 ttgatccagt acgtctgagc aacaaggaat tacgttggtt agcgagcaaa aaaaatcttg
      901 tgattgcagt acacaagtcc caaacggcta ccttgttgca taccgattcg cagcaacagg
      961 ttcgtggtat taatgctgat tatttaaatc ttttaaaaag agcgttaaat atcaaattaa
     1021 cactccggga atacgccgat catcaaaaag caatggacgc gcttgcagaa ggtgaagtcg
     1081 atatagtgtt atcacattta gttacttcgc cgcctcttaa tagtgacatt gctgcaacca
     1141 aaccattgat aattaccttt ccggcgctgg taaccaccct tcacgactca atgcgaccgc
     1201 ttacctcacc aaaaccagta aatattgctc gggtagcaaa ttacccccca gacgaggtaa
     1261 ttcatcaatc gtttccaaaa gcaacaatta tctcttttac aaatttatat caggcattag
     1321 catccgtatc agccggacag aatgattact ttattggtag taacatcatt accagcagta
     1381 tgatttcc
//
LOCUS       DAZKHF020000513         1375 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2201S31241
            SAMN43267800-rid25106413.denovo.517, whole genome shotgun sequence.
ACCESSION   DAZKHF020000513 DAZKHF020000000
VERSION     DAZKHF020000513.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267800
            Sequence Read Archive: SRR30307263
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1375)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1375)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 1375)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350581.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350581.2
            Genome Coverage       :: 151x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:21:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,652
            CDSs (total)                      :: 5,557
            Genes (coding)                    :: 5,320
            CDSs (with protein)               :: 5,320
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 237
            CDSs (without protein)            :: 237
            Pseudo Genes (ambiguous residues) :: 0 of 237
            Pseudo Genes (frameshifted)       :: 102 of 237
            Pseudo Genes (incomplete)         :: 130 of 237
            Pseudo Genes (internal stop)      :: 52 of 237
            Pseudo Genes (multiple problems)  :: 41 of 237
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1375
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267800-rid25106413.denovo.517"
                     /serotype="O 26:H11"
                     /isolate="2201S31241"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-01"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            81..1283
                     /locus_tag="ACHOSC_RS25380"
                     /old_locus_tag="pgaptmp_005076"
     CDS             81..1283
                     /locus_tag="ACHOSC_RS25380"
                     /old_locus_tag="pgaptmp_005076"
                     /EC_number="1.14.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HGU4550819.1"
                     /translation="MERFDAIIIGAGAAGMFCSALAGQAGRRVLLIDNGKKPGRKILM
                     SGGGRCNFTNLYVEPGAYLSQNPHFCKSALARFTQWDFIDLVNKHGIAWHEKTLGQLF
                     CDDSAQQIVDMLVDECEKGNVTFRLRSEVLSVAKDETGFTLELNGMTVGCEKLVIATG
                     GLSMPGLGASPFGYKIAEQFGLNVLPTRAGLVPFTLHKPLLEELQVLAGVAVPSVITA
                     ENGIVFRENLLFTHRGLSGPAVLQISSYWQPGEFVSINLLPDVDLETFLNEQRNAHPN
                     QSLKNTLAVHLPKRLVERLQQLGQIPDVSLKQLNVRDQQALISTLTDWRVQPNGTEGY
                     RTAEVTLGGVDTNELSSRTMEARKVPGLYFIGEVMDVTGWLGGYNFQWAWSSAWACAQ
                     DLIAAKSS"
BASE COUNT          313 a          332 c          394 g          336 t
ORIGIN      
        1 ccttttagta atattgatgc tggtgcgacc actgaggaat ctttacaatt cacgcccgtt
       61 ttttctaaga ggagcgcaac gtggaaaggt ttgatgccat tattataggc gctggtgcgg
      121 cgggtatgtt ctgttctgcg ctggcaggtc aggcaggacg ccgggttctg ctgatcgata
      181 atggtaaaaa accagggcgc aaaatcctta tgtctggcgg tgggcgctgc aactttacca
      241 acctttatgt cgaaccaggc gcttatctga gccagaatcc gcatttttgt aagtctgcac
      301 tcgcgcgttt tacccagtgg gatttcattg atctggtcaa taaacacggc atcgcctggc
      361 acgagaaaac gttaggacaa ctcttctgcg atgactccgc gcagcagatt gtcgacatgc
      421 tggtggatga gtgcgagaag ggcaatgtaa ccttcagatt gcgtagcgaa gtgctgagtg
      481 tggcgaagga tgaaacaggc ttcacgcttg aactgaacgg catgactgtc ggttgcgaaa
      541 agctggtcat cgcgaccggt gggctgtcaa tgccggggct gggcgcgtcg ccgtttggtt
      601 ataagattgc cgaacaattt ggcctcaacg tgctgccgac ccgcgcgggt ctggtgccat
      661 tcactctgca taaaccgttg ctcgaagagt tacaggtgct ggcgggcgtg gcggtgcctt
      721 ccgtgattac cgctgaaaac ggcatcgttt tccgtgagaa cttactcttc acccatcgcg
      781 gcttgtctgg accggcggtg ttgcagattt ccagctactg gcaaccgggt gaatttgtca
      841 gtatcaatct gctaccggat gtggatctcg aaaccttcct gaatgagcag cgtaacgcac
      901 atccgaatca gagcctgaaa aacacactgg cggttcatct accgaagcgg ttggttgaac
      961 gcttacagca actcgggcaa atcccggatg tttcgctaaa acagctcaac gtgcgtgacc
     1021 aacaggcact gattagcaca ttgaccgact ggcgcgtaca acccaacggc actgaaggct
     1081 atcgcactgc cgaagtgacg ctcggcggcg tggacaccaa cgaactctct tcacggacga
     1141 tggaagcgcg caaagtgcct gggctgtact tcatcggcga agtgatggac gttaccggct
     1201 ggctgggggg ctataacttc cagtgggcgt ggtcgagtgc gtgggcttgt gcgcaggatt
     1261 tgattgcagc gaagtcgtcc tgacctgtta cctaaagatg aaaattatta gagccgcatt
     1321 aaaaatgagc taattttgat agtggctatc ttgtgattat tttctaatga gcccg
//
LOCUS       DAZKHF020000514         1362 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2201S31241
            SAMN43267800-rid25106413.denovo.518, whole genome shotgun sequence.
ACCESSION   DAZKHF020000514 DAZKHF020000000
VERSION     DAZKHF020000514.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267800
            Sequence Read Archive: SRR30307263
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1362)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1362)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 1362)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350581.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350581.2
            Genome Coverage       :: 151x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:21:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,652
            CDSs (total)                      :: 5,557
            Genes (coding)                    :: 5,320
            CDSs (with protein)               :: 5,320
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 237
            CDSs (without protein)            :: 237
            Pseudo Genes (ambiguous residues) :: 0 of 237
            Pseudo Genes (frameshifted)       :: 102 of 237
            Pseudo Genes (incomplete)         :: 130 of 237
            Pseudo Genes (internal stop)      :: 52 of 237
            Pseudo Genes (multiple problems)  :: 41 of 237
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1362
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267800-rid25106413.denovo.518"
                     /serotype="O 26:H11"
                     /isolate="2201S31241"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-01"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            complement(<1..417)
                     /locus_tag="ACHOSC_RS25385"
                     /old_locus_tag="pgaptmp_005077"
     CDS             complement(<1..417)
                     /locus_tag="ACHOSC_RS25385"
                     /old_locus_tag="pgaptmp_005077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000031374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ERF family protein"
                     /protein_id="HGU4550820.1"
                     /translation="MSKDFYARLAAIQENLNAPKNQYNSFGKYKYRSCEDILEGVKPL
                     LNGLFLSISDEVVLIGDRYYVKATATITDGENSHTATALAREEESKKGMDSAQVTGAT
                     SSYARKYCLNGLFGIDDAKDADTDEHKHQQNAAAKQS"
     gene            complement(428..598)
                     /locus_tag="ACHOSC_RS25390"
                     /old_locus_tag="pgaptmp_005078"
     CDS             complement(428..598)
                     /locus_tag="ACHOSC_RS25390"
                     /old_locus_tag="pgaptmp_005078"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4550821.1"
                     /translation="MSLATTVKESKLQRRMYTQQALMYRQKGDREGVRVFLNAAKTEV
                     LNQRYFLGPCPF"
     gene            complement(674..826)
                     /gene="kil"
                     /locus_tag="ACHOSC_RS25395"
                     /old_locus_tag="pgaptmp_005079"
     CDS             complement(674..826)
                     /gene="kil"
                     /locus_tag="ACHOSC_RS25395"
                     /old_locus_tag="pgaptmp_005079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001243351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="host cell division inhibitory peptide Kil"
                     /protein_id="HGU4550822.1"
                     /translation="MRNEIAINHQMLRAAQNKAVIARFIGDSKMWLEANKAMKSAINL
                     PWYRRK"
     gene            complement(811..945)
                     /locus_tag="ACHOSC_RS25400"
                     /old_locus_tag="pgaptmp_005080"
     CDS             complement(811..945)
                     /locus_tag="ACHOSC_RS25400"
                     /old_locus_tag="pgaptmp_005080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016248931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4550823.1"
                     /translation="MMHFQLAGSGVMSAFYPHESELSRRVKQLIRAAKKQLEALCAMK
                     "
     gene            complement(1021..1332)
                     /locus_tag="ACHOSC_RS25405"
                     /old_locus_tag="pgaptmp_005081"
     CDS             complement(1021..1332)
                     /locus_tag="ACHOSC_RS25405"
                     /old_locus_tag="pgaptmp_005081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000776959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="superinfection exclusion protein"
                     /protein_id="HGU4550824.1"
                     /translation="MKLRVWHIPQVPMKPFIAEVASVEEGVRLMGALADYDAFQYDNN
                     IKPDYCNANGLEMWDESLTDEDLSEVGLTDRWVDWYSECQCYDDPRKYLESLKEETSA
                     A"
BASE COUNT          322 a          336 c          276 g          428 t
ORIGIN      
        1 tgactgcttt gctgctgcgt tctgctgatg tttatgctcg tcggtatctg catctttcgc
       61 atcatcaatg ccgaacaaac cattgaggca atacttgcgt gcataagagc ttgtagctcc
      121 cgtaacttgt gcagaatcca ttcctttctt gctttcttcc tctcgtgcaa gagcggttgc
      181 cgtatgactg ttttcgccat cggtaatagt tgccgtggct ttcacataat accgatcacc
      241 aatcaacaca acttcatcgc tgattgataa aaacaggcca ttcagtaacg gcttaacgcc
      301 ttcaagaatg tcttcgcagc ttctgtattt atatttaccg aatgagttgt actgattctt
      361 tggcgcgttc aagttctcct gaatagctgc cagtcttgcg taaaagtctt tgctcatatg
      421 tttgttctca gaatgggcat ggcccaagga aataacgctg atttaatact tcagtctttg
      481 ccgcatttaa aaatacgcga acaccttcac gatctccctt ctggcgatac attaacgcct
      541 gctgcgtgta catgcgtctc tgtaacttgc tctccttcac tgtggttgca agtgacatga
      601 atatctcctt cgttaccgat taattctttc atctgacgaa tgaattcttc gtctgaccag
      661 ttatctgtga aactcatttc ctgcgatacc acggaaggtt gatagctgat ttcatcgctt
      721 tatttgcttc aagccacatt tttgaatcac caataaatct ggctattact gctttgtttt
      781 gtgccgcacg aagcatctgg tgattaatgg ctatttcatt gcgcataacg cctccagttg
      841 tttctttgct gctctaatta attgtttaac tcggcgtgat aattcagatt cgtgcgggta
      901 gaaagcggac atgacgccgc tacccgcgag ctgaaagtgc atcatgggta actccttata
      961 tttgattgca taacgaaaac gcctcgagtg aagcgttatt ggtatgcggt aaagccgcgc
     1021 ttaggcggct gatgtttctt ctttcaggct ttcgagatat ttacgtgggt cgtcgtaaca
     1081 ttggcattcg ctgtaccaat ccacccagcg atcagtaagc cccacctctg ataaatcttc
     1141 atcggtaagg ctctcatccc acatctcaag gccgttagca ttgcagtaat caggcttgat
     1201 gttgttgtca tactgaaagg cgtcataatc agccagtgcg cccatcaggc gaacaccctc
     1261 ttcaacactt gccacttctg caatgaacgg tttcataggt acttgcggga tatgccagac
     1321 acgtaatttc atatttcctc cgtcaaaaaa atcgccctca ca
//
LOCUS       DAZKHF020000515         1358 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2201S31241
            SAMN43267800-rid25106413.denovo.519, whole genome shotgun sequence.
ACCESSION   DAZKHF020000515 DAZKHF020000000
VERSION     DAZKHF020000515.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267800
            Sequence Read Archive: SRR30307263
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1358)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1358)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 1358)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350581.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350581.2
            Genome Coverage       :: 151x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:21:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,652
            CDSs (total)                      :: 5,557
            Genes (coding)                    :: 5,320
            CDSs (with protein)               :: 5,320
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 237
            CDSs (without protein)            :: 237
            Pseudo Genes (ambiguous residues) :: 0 of 237
            Pseudo Genes (frameshifted)       :: 102 of 237
            Pseudo Genes (incomplete)         :: 130 of 237
            Pseudo Genes (internal stop)      :: 52 of 237
            Pseudo Genes (multiple problems)  :: 41 of 237
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1358
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267800-rid25106413.denovo.519"
                     /serotype="O 26:H11"
                     /isolate="2201S31241"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-01"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            <1..>1358
                     /locus_tag="ACHOSC_RS25410"
                     /old_locus_tag="pgaptmp_005082"
     CDS             <1..>1358
                     /locus_tag="ACHOSC_RS25410"
                     /old_locus_tag="pgaptmp_005082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001480735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015473 - fimbrial usher porin activity
                     [Evidence IEA];
                     GO_process: GO:0009297 - pilus assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbria/pilus outer membrane usher protein"
                     /protein_id="HGU4550825.1"
                     /translation="GCASAYAVEFNKDLIEAEDRENVNLSQFETDGQLPVGKYSLNAL
                     INNKRTPIHLDLQWVLIDNQTAVCLTPEQLTLLGFTDEIIEEAQQNLIDGCYPIEKEK
                     QITTYLDKGKMQLSISAPQAWLKYKDANWTPPELWDHGIAGAFLDYNLYASHYAPHQG
                     DNSQNISSYGQAGVNLGAWRLRTDYQYDQSFNNGKSQANNLDFPRIYLFRPIPAINAK
                     LTIGQYDTESSIFDSFHFSGVSLKSDENMLPPDLRGYAPQITGVAQTNAKVTVSQNNR
                     IIYQENVPPGPFAITNLFNTLQGQLDVKVEEEDGQVTQWQVASNSIPYLTRKGQIRYT
                     TAMGKPTSVGGDSLQQPFFWTGEFSWGWLNNVSLYGGSVLTNRDYQSLAAGVGFNLNS
                     LGSLSFDVTRSDAQLHNQDKETGYSYRANYSKRFESTGSQLTFAGYRFSDKNFVTMNE
                     YI"
BASE COUNT          399 a          291 c          275 g          393 t
ORIGIN      
        1 ggttgtgcgt cagcttatgc cgttgaattc aacaaagatt taatcgaagc cgaagatcgt
       61 gaaaacgtta acctttccca atttgaaact gatggccaat tacccgtagg taaatactca
      121 ttaaacgctc ttattaataa taaaaggacg ccaatccatc tggacctgca atgggtatta
      181 attgataacc aaactgcagt ttgtctgact ccagagcaat taacattatt aggctttact
      241 gatgagatta ttgaagaggc tcagcaaaac ctgatcgatg gttgttaccc tatcgaaaaa
      301 gaaaaacaaa ttacaactta tctcgataaa gggaaaatgc aattatccat atctgcacct
      361 caggcatggt taaaatacaa agatgcaaac tggacgcctc ctgaactttg ggatcatggt
      421 attgctgggg catttcttga ctacaattta tatgcctctc attatgcacc acatcagggc
      481 gataattcac aaaatataag ttcctatggg caggctgggg ttaatcttgg ggcctggcgc
      541 ctgcgtactg attaccagta cgatcagtca tttaacaatg gcaaaagcca ggcgaacaac
      601 ctggattttc cgcgtattta tttgtttcgc ccaatcccag caattaatgc aaaactaact
      661 ataggtcaat acgatactga atcctctatt ttcgactctt tccatttttc tggcgtttcg
      721 ttgaaaagcg atgagaatat gttgccgcct gatctacgtg gttacgcacc gcaaatcacg
      781 ggtgttgcac aaacgaatgc aaaggtcact gtctcacaga acaaccgtat tatttatcaa
      841 gaaaatgttc ctcctggccc atttgctatt actaatttat tcaatacatt acaggggcaa
      901 ctggacgtca aggttgaaga agaggacggg caagtcacgc aatggcaagt tgcatctaat
      961 agtattcctt atctgacgcg taaagggcag attcgctaca ccactgctat gggtaaaccg
     1021 accagcgttg gtggtgattc cttacaacaa cccttcttct ggactggtga attctcatgg
     1081 ggttggctga acaatgtatc cctgtatggt ggttcagttt taacaaaccg tgattatcaa
     1141 tctctggctg ccggcgttgg ctttaacctt aactcattgg gttcattatc ttttgatgtc
     1201 acacgatctg atgctcagtt gcataatcag gataaagaaa cgggttatag ctaccgcgct
     1261 aactattcaa aacgttttga atctaccggt agccagctca ctttcgctgg ttaccgtttc
     1321 tctgataaaa actttgtgac aatgaatgaa tatatcaa
//
LOCUS       DAZKHF020000516         1355 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2201S31241
            SAMN43267800-rid25106413.denovo.520, whole genome shotgun sequence.
ACCESSION   DAZKHF020000516 DAZKHF020000000
VERSION     DAZKHF020000516.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267800
            Sequence Read Archive: SRR30307263
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1355)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1355)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 1355)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350581.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350581.2
            Genome Coverage       :: 151x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:21:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,652
            CDSs (total)                      :: 5,557
            Genes (coding)                    :: 5,320
            CDSs (with protein)               :: 5,320
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 237
            CDSs (without protein)            :: 237
            Pseudo Genes (ambiguous residues) :: 0 of 237
            Pseudo Genes (frameshifted)       :: 102 of 237
            Pseudo Genes (incomplete)         :: 130 of 237
            Pseudo Genes (internal stop)      :: 52 of 237
            Pseudo Genes (multiple problems)  :: 41 of 237
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1355
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267800-rid25106413.denovo.520"
                     /serotype="O 26:H11"
                     /isolate="2201S31241"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-01"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            complement(89..1318)
                     /locus_tag="ACHOSC_RS25415"
                     /old_locus_tag="pgaptmp_005083"
     CDS             complement(89..1318)
                     /locus_tag="ACHOSC_RS25415"
                     /old_locus_tag="pgaptmp_005083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000085269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HGU4550826.1"
                     /translation="MSRALNKLSDTQLRKINGTPAQKTAFLNDGGNLSVRHSTSGLLT
                     WYFTYRAGTGRGARPERIKLGNYPDLSLKAAREKAAQCRAWLAEGKNPRHEMNYTVQK
                     ALKPVTVGDALTYWLESYVKENRVDYAALKKRLNNHVIQHIGAMPLDKCELRHWLACF
                     DQVAKRTPVTAGFLLQTCKQALKFCRRRRYAISNVLDDMSVADVGKKPDISERVLSTK
                     ELGELLQALDKKIFSPYYVALIRLLIVFGCRTVELRLSEISEWDFTEMLWTVPKEHSK
                     TKVAIFRPIPEAILPFVTQLVEQNRHTGLLLGEVKQETSVSQYGRLAHRRLKHPHWSL
                     HDIRRTFTTMLNDLGVDPHVVEQLTGHQMPGMQRVYNHSRYLDAKRDALDMWTERLGI
                     LAGTHENVTTLPIAREI"
BASE COUNT          281 a          367 c          309 g          398 t
ORIGIN      
        1 taacccgttg ttccattttg ttccttgttg ttcgtcgttg tgtctcgttg tttcacgttg
       61 tccgcatcct gaaaaatcgc aaaaaaaatt atatttctct ggctattggc aatgtggtta
      121 cgttttcatg tgttcccgcc agtatcccta accgctccgt ccacatatcc agcgcgtcac
      181 gtttcgcatc cagataacgg gaatggttat agactcgttg catccctggc atctgatgac
      241 ctgtaagctg ctccacgacg tgaggatcca cgcctaaatc gttcagcatc gttgtaaagg
      301 tgcgccggat gtcatgcagt gaccagtgag ggtgttttag cctcctgtgc gctaatctgc
      361 cgtactgcga cacgcttgtt tcctgtttca cttcccccag caataagccc gtgtgcctgt
      421 tctgctccac cagctgcgtg acgaacggca gtattgcttc cggtatgggc cggaatattg
      481 ccaccttcgt tttgctgtgt tctttcggca cggtccagag catttcggta aaatcccact
      541 cgctgatctc cgataacctc agttctaccg tccggcatcc aaaaacaatc aggaggcgga
      601 ttagtgcgac gtagtaaggg gaaaatattt ttttgtccag tgcctgcaat aattcgccaa
      661 gttctttggt gcttaagaca cgctcgctta tatccggttt tttcccaacg tccgccacac
      721 tcatatcatc aagaacgttg ctgattgcat agcgccgcct ccggcagaac ttaagcgcct
      781 gtttgcacgt ctgtagcaag aatccggcag taacaggcgt tcgctttgcc acctggtcaa
      841 aacaggccag ccagtgccgt agctcgcatt tatccagcgg catagcacca atgtgctgta
      901 ttacgtgatt attaaggcgc tttttcaggg cggcataatc cacgcggttt tcctttacgt
      961 aagactcaag ccagtaggta agcgcatcgc caaccgttac gggctttaac gctttctgta
     1021 cggtgtaatt catctcatga cgtggatttt tcccctcagc aagccaagcg cgacactgtg
     1081 ccgctttttc cctggcagct ttcagactca gatcaggata atttcccagc ttaatccgtt
     1141 ccggtcgtgc tccccttccc gttccggccc tgtaggtgaa ataccaggtt aaaaggccgc
     1201 tggttgaatg cctgacgctc aggtttccac cgtcattaag aaaggctgtt ttttgggcgg
     1261 gtgtgccgtt gattttcctc agctgtgtat cgctcagttt gttaagtgct ctgctcataa
     1321 ttttgattcc ggtcatcaca attaactaca ccatt
//
LOCUS       DAZKHF020000517         1351 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2201S31241
            SAMN43267800-rid25106413.denovo.521, whole genome shotgun sequence.
ACCESSION   DAZKHF020000517 DAZKHF020000000
VERSION     DAZKHF020000517.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267800
            Sequence Read Archive: SRR30307263
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1351)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1351)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 1351)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350581.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350581.2
            Genome Coverage       :: 151x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:21:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,652
            CDSs (total)                      :: 5,557
            Genes (coding)                    :: 5,320
            CDSs (with protein)               :: 5,320
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 237
            CDSs (without protein)            :: 237
            Pseudo Genes (ambiguous residues) :: 0 of 237
            Pseudo Genes (frameshifted)       :: 102 of 237
            Pseudo Genes (incomplete)         :: 130 of 237
            Pseudo Genes (internal stop)      :: 52 of 237
            Pseudo Genes (multiple problems)  :: 41 of 237
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1351
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267800-rid25106413.denovo.521"
                     /serotype="O 26:H11"
                     /isolate="2201S31241"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-01"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            complement(<1..152)
                     /locus_tag="ACHOSC_RS25420"
                     /old_locus_tag="pgaptmp_005084"
     CDS             complement(<1..152)
                     /locus_tag="ACHOSC_RS25420"
                     /old_locus_tag="pgaptmp_005084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TrmB family transcriptional regulator"
                     /protein_id="HGU4550827.1"
                     /translation="MLKQQDMTETARVVFDELSVTEPATVGEIAQNTYLSRERCQLIL
                     TQLVMAG"
     gene            complement(439..1257)
                     /locus_tag="ACHOSC_RS25425"
                     /old_locus_tag="pgaptmp_005085"
     CDS             complement(439..1257)
                     /locus_tag="ACHOSC_RS25425"
                     /old_locus_tag="pgaptmp_005085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysostaphin resistance A-like protein"
                     /protein_id="HGU4550828.1"
                     /translation="MWIVLVLSLSTLSWHKVVAFSLLTVSVVLAVLNDIIDWSVLFFV
                     ATIVFFIILKFNWKYNAWAKSIYEVGIVLSAIALSFHLWPGFHNPVVLNSVTVGPQST
                     PYTMYFNFDKALVPFLLVLCTSSLFKKEVKSEVSLWKWGALSLSVPLILFLAVFFGGL
                     KPEIHFPEWLPEFILANLFFVSLAEESLFRGYIQSRLSEVTSPLVALIVAALLFGFYH
                     YSGGALLVLFATLSGVVYGLSWMWSGRLWVATLFHFGLNLCHLLFFTYPFLKHN"
BASE COUNT          494 a          316 c          219 g          322 t
ORIGIN      
        1 cccgccataa caagctgggt cagtattaac tggcagcgtt cgcgtgaaag gtaagtattc
       61 tgcgcaatct ccccgactgt cgccggttcg gtgacgctta attcatcaaa cacaactctg
      121 gcggtttctg tcatatcctg ctgtttcagc atgtcttttt accctttccg gttaacgtga
      181 cacaccaata actcttgccg aaaaagccag caagctgaaa gacctgtatt aataactacc
      241 agcacattta acgcactgcg ctactttgcg ggcacaaaaa acccgctcag aggcggggtc
      301 aagctatgcg gcgaaataac cactcttaac agcatacctg attttttacg tacgtaaatg
      361 ctttgccgtg catatttttc atgcaaatgt cacgtcctgc tatttttcag tcttataaat
      421 ttaaaaccat agaaaaaatc aattatgttt taaaaatgga taggtaaaga ataacaagtg
      481 acacagattc aaaccaaaat ggaaaagggt ggcaacccac aaacgcccac tccacatcca
      541 tgacaatcca tacacaacac cagataacgt ggcaaataat acaagtaaag caccacctga
      601 atagtgataa aaaccaaaca acaaagccgc cacaattaat gcaaccaatg gagacgttac
      661 ttctgatagc cgtgattgaa tataccctct aaataatgat tcctctgcca gagacacaaa
      721 aaacaaatta gccaatataa actctggcaa ccactcagga aaatgaatct ctggctttaa
      781 tccaccaaaa aaaacagcca aaaacaggat aagaggaaca gagagcgaca gagcccccca
      841 cttccacaaa gacacttctg attttacttc ttttttaaac aaagaagatg tacacaggac
      901 taacaaaaat ggcaccagcg ctttatcaaa attaaaatac attgtatagg gagtactctg
      961 agggccaaca gtaacagaat ttagcactac aggattgtga aaccctggcc atagatggaa
     1021 agataatgct atggctgata aaactatgcc aacttcatat atagatttag cccaggcgtt
     1081 atatttccag ttgaacttca aaataataaa aaaaacgatt gtagcaacaa aaaataacac
     1141 cgaccaatca ataatatcat taagcacagc caggacaaca gacaccgtca acaatgaaaa
     1201 agccactacc ttatgccaac tgagagttga cagtgacagt actaacacaa tccacataag
     1261 cactcctttt atttaatgat gaagattgat tatcaatatt ttcaattcac caggcaacat
     1321 tttatctacc ttccacaata catgaccatc a
//
LOCUS       DAZKHF020000518         1351 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2201S31241
            SAMN43267800-rid25106413.denovo.522, whole genome shotgun sequence.
ACCESSION   DAZKHF020000518 DAZKHF020000000
VERSION     DAZKHF020000518.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267800
            Sequence Read Archive: SRR30307263
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1351)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1351)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 1351)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350581.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350581.2
            Genome Coverage       :: 151x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:21:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,652
            CDSs (total)                      :: 5,557
            Genes (coding)                    :: 5,320
            CDSs (with protein)               :: 5,320
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 237
            CDSs (without protein)            :: 237
            Pseudo Genes (ambiguous residues) :: 0 of 237
            Pseudo Genes (frameshifted)       :: 102 of 237
            Pseudo Genes (incomplete)         :: 130 of 237
            Pseudo Genes (internal stop)      :: 52 of 237
            Pseudo Genes (multiple problems)  :: 41 of 237
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1351
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267800-rid25106413.denovo.522"
                     /serotype="O 26:H11"
                     /isolate="2201S31241"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-01"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            complement(240..848)
                     /locus_tag="ACHOSC_RS25430"
                     /old_locus_tag="pgaptmp_005086"
     CDS             complement(240..848)
                     /locus_tag="ACHOSC_RS25430"
                     /old_locus_tag="pgaptmp_005086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001415151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P22AR C-terminal domain-containing protein"
                     /protein_id="HGU4550829.1"
                     /translation="MVTQLAFRDVKFTPINHNNQIWFTSKELAAALKYASTKAVTDIY
                     NKNIDEFTDGMSQVVESTTSGNYRKKTRIFSLRGAHLIAMFARTPVAKEFRRWVLDIL
                     DREIQQSPITKQFTDNELCTLAWLWRASDTMLTACQNVTPLLQVAEHREAGRFTSIEQ
                     EYPRILNKAQAILARETAHVKFRPWQDDKWSRVLTHLRSERL"
     gene            complement(1274..1342)
                     /locus_tag="ACHOSC_RS25435"
                     /old_locus_tag="pgaptmp_005087"
                     /pseudo
     CDS             complement(1274..1342)
                     /locus_tag="ACHOSC_RS25435"
                     /old_locus_tag="pgaptmp_005087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165308.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT          347 a          314 c          315 g          375 t
ORIGIN      
        1 gaactgaaaa caatattgga tatcattttc ggtatttctc tgcaaattcc agcccacagg
       61 atctcggtgt acaatctgtc gctgatcgcg caaatttagg gtcaatcact tcattttttg
      121 catcaaaccc tcactaacat cgaacgatta tagtacaagt agtgttcttg acttctcatt
      181 aaaccgtcag cattcaatta attacaaaat actagcggca tttctctccc gcaactttat
      241 tacagccgtt cagaacgtaa atgcgtcaat actcgactcc acttatcatc ctgccacggc
      301 cggaatttta catgtgccgt ttctctggca aggattgcct gtgccttatt gagtatccgg
      361 ggatattctt gttcgattga agtgaagcga ccggcttcgc ggtgttctgc tacctgaaga
      421 agcggagtga cgttttggca agcggttaac attgtgtcac ttgcccgcca taaccaagca
      481 agtgtgcaaa gttcgttatc agtgaattgt tttgtgattg gggattgttg aatttctcga
      541 tcgagaatat ccagcaccca gcggcggaat tctttggcca caggagtgcg ggcgaacatg
      601 gcgatcagat gggcgccacg gagggagaaa atgcgagtct tcttacgata atttcctgag
      661 gtcgttgatt cgacgacctg agacattccg tcagtaaact catcaatatt tttgttgtag
      721 atgtcggtta ctgctttggt gctggcatat tttaatgctg ccgccaattc tttgctggta
      781 aaccagatct ggttgttgtg atttatagga gtgaatttta catcgcggaa tgcgagctga
      841 gtgaccatag cgataatctc gttttgaatt tgagctatca ccaccgacga cgccaatcga
      901 ctggtggtgg actgtgcaag gttggcgtaa ccggtcaaaa cgaaatccgg cgcttccgaa
      961 gaagccctca cacagcccac cataattttt gtgtagacgt gcactgcgca taaaaaaacc
     1021 gcgaacgcgg ttatgcgtcg ttttgatgtc cgggacgcca atcccgtgtg ccgattttgc
     1081 ggcaacgcac agaatatagc gccggataaa taatgtcgtc aacccttgca tatggggggc
     1141 gcgatgatca ctactaccac cataatcgct ccaactgttg caactacttt aggctaagac
     1201 atcacgattt ccatatcacc tttagccgcc agacaaacag agactccctg ttcttggctg
     1261 ccaaaaacac cgaatgcaaa aaatcgcagg tgtatttctg catctgtgga gggagaagag
     1321 agtttggatt gtatgtgcgc ataaacgtcc c
//
LOCUS       DAZKHF020000519         1344 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2201S31241
            SAMN43267800-rid25106413.denovo.523, whole genome shotgun sequence.
ACCESSION   DAZKHF020000519 DAZKHF020000000
VERSION     DAZKHF020000519.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267800
            Sequence Read Archive: SRR30307263
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1344)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1344)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 1344)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350581.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350581.2
            Genome Coverage       :: 151x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:21:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,652
            CDSs (total)                      :: 5,557
            Genes (coding)                    :: 5,320
            CDSs (with protein)               :: 5,320
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 237
            CDSs (without protein)            :: 237
            Pseudo Genes (ambiguous residues) :: 0 of 237
            Pseudo Genes (frameshifted)       :: 102 of 237
            Pseudo Genes (incomplete)         :: 130 of 237
            Pseudo Genes (internal stop)      :: 52 of 237
            Pseudo Genes (multiple problems)  :: 41 of 237
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1344
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267800-rid25106413.denovo.523"
                     /serotype="O 26:H11"
                     /isolate="2201S31241"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-01"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            1..1015
                     /locus_tag="ACHOSC_RS25440"
                     /old_locus_tag="pgaptmp_005088"
                     /pseudo
     CDS             1..1015
                     /locus_tag="ACHOSC_RS25440"
                     /old_locus_tag="pgaptmp_005088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001744394.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
     gene            complement(1058..1258)
                     /gene="glgS"
                     /locus_tag="ACHOSC_RS25445"
                     /old_locus_tag="pgaptmp_005089"
     CDS             complement(1058..1258)
                     /gene="glgS"
                     /locus_tag="ACHOSC_RS25445"
                     /old_locus_tag="pgaptmp_005089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell surface composition regulator GlgS"
                     /protein_id="HGU4550830.1"
                     /translation="MDHSLNSLNNFDFLARSFARMHAEGRPVDILAVTGNMDEEHRTW
                     FCARYAWYCQQMMQARELELEH"
BASE COUNT          404 a          280 c          272 g          388 t
ORIGIN      
        1 cctgtatggg caatggactg ctatgctgaa catgaagggg gaaataccgt agtcattggt
       61 tacgtaccaa gaatttctat ccccagtaat ggtaaaaaag gtgataagat ctggcaaagc
      121 agtgagtatt ttatgaatgt cttctgtaat aatgcattac ctgccccatc acctggagaa
      181 gaatacccat cagcctggac taatataatg atgtttttac caggcggtca ggacttttat
      241 aatcagaact cctatatttt tggcgtaaca tacaatggag ttgattatga ttcgactgcc
      301 cctaatgcta tcgcagcacc agaatgcatt gatataaaag gagcgggtac ctttaataat
      361 cactataaaa aaccccgctg tctgtagtgg aggtcctgag cctcaattat cagtaacctt
      421 tccagctcgc gtacaactct atattaaatt agcaaaaaat gccaatagag ttaataaaaa
      481 cctggtgtta cctgatgaat atatcgctct agagtttaaa ggtatgagtg gcgcaggagc
      541 tatagaagta gacaaaaatt tgaccttcag gattcgtgga ttaaataata ttcatgtcct
      601 tgactgcttt gttaatgtcg cactggaacc agctgatggc gttgttgact ttggtaaaat
      661 aaattcccga acaattaaaa ataccagcgt tagtgagacg tttagtgtag tcatgacaaa
      721 agatcctggt gcggcctgta ctgagcaatt caatatttta gggagctttt tcactacgga
      781 tattttgagt gattatagcc atctggatat gggtaatgga ctgctattga agatatttca
      841 taaagatgga acagcaacgg aatttaaccg cttctcacaa tttgcttctt tttcatcgtc
      901 tagtgcacct tcggtcaccg caccattcag ggcagaactt agtgcgaatc cggcagagac
      961 tgttgttgag ggaccgttta gtaaagacgt catcctcaaa atcacctata actagcatct
     1021 aatacaaaca ctaaaacggg ccatcaggcc cgttatatca gtgctctaac tccagctctc
     1081 ttgcctgcat catctgttga caataccagg cataacgtgc gcaaaaccag gttctatgtt
     1141 cttcatccat gttaccagta acggcaagaa tatcgaccgg gcgaccttct gcgtgcattc
     1201 tggcaaaact acgcgccagg aaatcgaaat tatttaaaga attaagacta tgatccataa
     1261 gcacatcctc cggttatcgc atacctttta aggccatatg ctttttctga tttcagtatt
     1321 ccccaatcag tgtagtgcct ttaa
//
LOCUS       DAZKHF020000520         1344 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2201S31241
            SAMN43267800-rid25106413.denovo.524, whole genome shotgun sequence.
ACCESSION   DAZKHF020000520 DAZKHF020000000
VERSION     DAZKHF020000520.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267800
            Sequence Read Archive: SRR30307263
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1344)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1344)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 1344)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350581.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350581.2
            Genome Coverage       :: 151x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:21:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,652
            CDSs (total)                      :: 5,557
            Genes (coding)                    :: 5,320
            CDSs (with protein)               :: 5,320
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 237
            CDSs (without protein)            :: 237
            Pseudo Genes (ambiguous residues) :: 0 of 237
            Pseudo Genes (frameshifted)       :: 102 of 237
            Pseudo Genes (incomplete)         :: 130 of 237
            Pseudo Genes (internal stop)      :: 52 of 237
            Pseudo Genes (multiple problems)  :: 41 of 237
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1344
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267800-rid25106413.denovo.524"
                     /serotype="O 26:H11"
                     /isolate="2201S31241"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-01"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            complement(<1..348)
                     /locus_tag="ACHOSC_RS25450"
                     /old_locus_tag="pgaptmp_005090"
     CDS             complement(<1..348)
                     /locus_tag="ACHOSC_RS25450"
                     /old_locus_tag="pgaptmp_005090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1398 family protein"
                     /protein_id="HGU4550831.1"
                     /translation="MDQVVVFQKMFEQVRKEQNFSWFYSELKHHRIAHYIYYLATDNI
                     RIITHDDTVLLLRGTRNLLKVSTTKNPAKIKEAALLHICGKSTFREYCSTLAGAGVFR
                     WVTDVNHNKRSYYA"
     gene            complement(569..1300)
                     /gene="bluR"
                     /locus_tag="ACHOSC_RS25455"
                     /old_locus_tag="pgaptmp_005091"
     CDS             complement(569..1300)
                     /gene="bluR"
                     /locus_tag="ACHOSC_RS25455"
                     /old_locus_tag="pgaptmp_005091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional repressor BluR"
                     /protein_id="HGU4550832.1"
                     /translation="MAYYSIGDVAERCGINPVTLRAWQRRYGLLKPQRSEGGHRLFDE
                     EDIQRIEEIKRWISNGVPVGKVKALLETTSQDTEDDWSRLQEEMMSILRMANPAKLRA
                     RIISLGREYPVDQLINHVYLPVRQRLVLDHNTSRIMSSMFDGALIEYAATSLFEMRRK
                     PGKEAILMAWNVEERARLWLEAWRLSLSGWHISVLADPIESPRPELFPTQTLIVWTGM
                     APTRRQNELLQHWGEQGYKVIFHAP"
BASE COUNT          342 a          312 c          305 g          385 t
ORIGIN      
        1 ggcgtagtaa ctgcgtttgt tatgattaac atcagtaacc caccggaaaa cgcccgcgcc
       61 tgctagtgtt gaacagtatt cccgaaatgt agattttccg caaatatgaa gcaatgcggc
      121 ctcttttatt ttagcagggt tcttggtcgt actaactttt aacaggttcc tggttcctct
      181 taataacaaa accgtgtcat cgtgagtaat aattctgatg ttatccgtag ccagataata
      241 aatgtaatgt gcaatacggt gatgttttaa ttctgaataa aaccaggaga agttttgctc
      301 ttttctcact tgctcaaaca tcttttgaaa aacaacgacc tgatccatca ggataataac
      361 ctcttgttag ttgtgagact gcgtagtgtg cacgatcggt tttaccactt caatctggtc
      421 tgtcctttgg ctgtgatatg tacagagtgt gatagaggga atatctgaat tctcccggtg
      481 agcattttgc aacggaccag ctccggtaca aacgctgttg tgggttcaga ttataacatt
      541 ctgtctaagg ggcgggataa aggtgaaatt agggggcatg aaagatgact ttataacctt
      601 gctcacccca atgttgtaaa agttcgtttt gccttctcgt tggtgccatg cctgtccaga
      661 caatcaatgt ttgcgtcggg aacagttccg ggcgcggcga ttcaatggga tcagcaagaa
      721 cagaaatgtg ccatcctgac aatgataaac gccatgcttc cagccacaat cgtgctctct
      781 cttcaacatt ccacgccatc agaatggctt ctttaccggg cttacggcgc atttcgaaaa
      841 gcgaggttgc tgcgtattca attaatgcgc cgtcaaacat actgctcata atgcgggagg
      901 tgttgtgatc aagcacgaga cgctggcgaa caggaaggta aacatgatta atcaattgat
      961 ctactgggta ctctcgaccc agtgaaataa ttctcgcgcg tagtttggca ggattagcca
     1021 tgcgaagaat tgacatcatc tcttcttgca ggcggctcca gtcatcttcc gtatcctggc
     1081 tggtggtttc cagtaatgct ttaactttgc ctacagggac gccattactt atccaacgct
     1141 tgatctcttc gatgcgttgt atgtcttctt catcaaagag tcggtgtccg ccttcactgc
     1201 gctgtggttt taacaaaccg tagcggcgtt gccaggcccg gagagtgaca ggattaatcc
     1261 cgcaacgttc agcaacatca ccaatgctgt aataagccac aattcctcct tgcggtcaca
     1321 aatctccgtc gcctgtacac gacc
//
LOCUS       DAZKHF020000521         1340 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2201S31241
            SAMN43267800-rid25106413.denovo.525, whole genome shotgun sequence.
ACCESSION   DAZKHF020000521 DAZKHF020000000
VERSION     DAZKHF020000521.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267800
            Sequence Read Archive: SRR30307263
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1340)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1340)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 1340)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350581.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350581.2
            Genome Coverage       :: 151x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:21:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,652
            CDSs (total)                      :: 5,557
            Genes (coding)                    :: 5,320
            CDSs (with protein)               :: 5,320
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 237
            CDSs (without protein)            :: 237
            Pseudo Genes (ambiguous residues) :: 0 of 237
            Pseudo Genes (frameshifted)       :: 102 of 237
            Pseudo Genes (incomplete)         :: 130 of 237
            Pseudo Genes (internal stop)      :: 52 of 237
            Pseudo Genes (multiple problems)  :: 41 of 237
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1340
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267800-rid25106413.denovo.525"
                     /serotype="O 26:H11"
                     /isolate="2201S31241"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-01"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            <1..500
                     /locus_tag="ACHOSC_RS25460"
                     /old_locus_tag="pgaptmp_005092"
     CDS             <1..500
                     /locus_tag="ACHOSC_RS25460"
                     /old_locus_tag="pgaptmp_005092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000450870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF1627 domain-containing protein"
                     /protein_id="HGU4550833.1"
                     /translation="EFIGQDGAKTCEELAGKFGVSTRKVASTLAVVTATGRLARVNQN
                     GKFRYCMSGGNLPADPKAAPVTKNDGKAFPQPAGAALPVREAATQEEIKTESVAVTVQ
                     SQPSFTRKHPDGLILPSLHVANRELRRAKGQVQKWERVCAALRELNKCRDILRDITAT
                     REQQR"
     gene            533..814
                     /locus_tag="ACHOSC_RS25465"
                     /old_locus_tag="pgaptmp_005093"
     CDS             533..814
                     /locus_tag="ACHOSC_RS25465"
                     /old_locus_tag="pgaptmp_005093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HGU4550834.1"
                     /translation="MILRQCAGTMKVKSVGALIGRTEAAVRTKARELGISMMLRGDFH
                     PSAKYSQRDIELARQLHQRGMQRREIARKLGMPLRIVNNYVYFDRRVSA"
     gene            811..1038
                     /locus_tag="ACHOSC_RS25470"
                     /old_locus_tag="pgaptmp_005094"
     CDS             811..1038
                     /locus_tag="ACHOSC_RS25470"
                     /old_locus_tag="pgaptmp_005094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4550835.1"
                     /translation="MKILYQDYGPVGQVVISSTVMEFRKHNRVVDAVLLTCPGISASR
                     AGVFIMKTKLYGSKAWIKKAYRVALQEVNSE"
     gene            1031..>1340
                     /locus_tag="ACHOSC_RS25475"
                     /old_locus_tag="pgaptmp_005095"
     CDS             1031..>1340
                     /locus_tag="ACHOSC_RS25475"
                     /old_locus_tag="pgaptmp_005095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4550836.1"
                     /translation="MSEIKEMPVVRDGYGYWTHPEYEKFCDGREYISTEEFNAWMEEN
                     NLQYVLCFRDEGCADLDACDADISAWEPERPEGDGWFIGSIHDTEDGPVCVWLRNKAE
                     A"
BASE COUNT          346 a          263 c          428 g          303 t
ORIGIN      
        1 ttgagtttat cggtcaggat ggtgctaaaa cgtgtgagga actggcgggt aagttcggcg
       61 tcagtactcg caaggttgct tccacgctgg cggtggtaac cgcaacgggg cggctggcac
      121 gcgttaatca gaacggtaaa tttcgttact gcatgtcggg gggtaattta ccagcagatc
      181 cgaaagccgc gccggtaacg aaaaatgatg gtaaggcctt tcctcagcca gcaggtgctg
      241 cgttaccagt ccgggaagcc gcaacacagg aagaaataaa aacggaaagt gtggcggtca
      301 cagtgcagtc acagccgtcg ttcaccagaa agcatccgga tggtctgatt ttaccatcgc
      361 tgcatgtggc taaccgcgag ctgcgccggg caaaaggtca ggttcagaag tgggagcgag
      421 tctgcgccgc gctgcgggag ctgaacaagt gccgggatat tctccgggat attaccgcca
      481 ccagagaaca gcagcggtga gtgggtggaa gacgtggtgc cgggcggaaa tcatgatact
      541 ccggcagtgt gcgggaacga tgaaggtaaa aagcgttggc gcacttatcg gacgaactga
      601 agcggcagtg agaacgaagg cacgggagct gggcatcagc atgatgttac gtggtgattt
      661 tcacccgtcg gcaaaatatt ctcagcgtga tattgagctg gcgcggcaac tgcatcagag
      721 aggcatgcaa agaagggaaa ttgccagaaa attaggcatg ccgctgcgca tagtgaataa
      781 ctacgtttat ttcgacagga gggtgtctgc gtgaaaatcc tgtatcagga ttacggcccg
      841 gtggggcagg tggttatcag cagtactgta atggagtttc ggaagcataa ccgtgtggtg
      901 gatgctgtgc tgttaacctg tccggggata tcggcgagtc gtgcaggtgt gtttattatg
      961 aagacgaaat tatatggcag taaggcgtgg ataaagaagg cgtatcgtgt agcgttgcag
     1021 gaggttaaca gtgagtgaaa ttaaagaaat gccggtagtt cgtgacggat atggctactg
     1081 gacacatcct gaatatgaaa aattctgtga tggtcgggaa tatatttcaa cggaagagtt
     1141 taacgcctgg atggaggaaa ataatcttca atacgtcctc tgcttcagag atgaaggatg
     1201 tgctgacctt gatgcgtgtg atgctgatat ttctgcatgg gaaccggaac gaccagaggg
     1261 cgatggctgg tttattggtt ccattcatga tacggaagat ggcccggttt gtgtctggtt
     1321 gcgaaataag gctgaagcat
//
LOCUS       DAZKHF020000522         1338 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2201S31241
            SAMN43267800-rid25106413.denovo.526, whole genome shotgun sequence.
ACCESSION   DAZKHF020000522 DAZKHF020000000
VERSION     DAZKHF020000522.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267800
            Sequence Read Archive: SRR30307263
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1338)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1338)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 1338)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350581.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350581.2
            Genome Coverage       :: 151x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:21:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,652
            CDSs (total)                      :: 5,557
            Genes (coding)                    :: 5,320
            CDSs (with protein)               :: 5,320
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 237
            CDSs (without protein)            :: 237
            Pseudo Genes (ambiguous residues) :: 0 of 237
            Pseudo Genes (frameshifted)       :: 102 of 237
            Pseudo Genes (incomplete)         :: 130 of 237
            Pseudo Genes (internal stop)      :: 52 of 237
            Pseudo Genes (multiple problems)  :: 41 of 237
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1338
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267800-rid25106413.denovo.526"
                     /serotype="O 26:H11"
                     /isolate="2201S31241"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-01"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            <1..845
                     /locus_tag="ACHOSC_RS25480"
                     /old_locus_tag="pgaptmp_005096"
     CDS             <1..845
                     /locus_tag="ACHOSC_RS25480"
                     /old_locus_tag="pgaptmp_005096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001735401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="polysaccharide biosynthesis protein"
                     /protein_id="HGU4550837.1"
                     /translation="NSVLNATRGADFLYHAAALKQVPSCEFHPMEAVKTNVLGTENVL
                     EAAIANGIKRVVCLSTDKAVYPINAMGISKAMMEKVIVAKSRNLDSSKTVICGTRYGN
                     VMASRGSVIPLFVDLIKAGKPLTITDPNMTRFMMTLEDAVDLVLYAFEHGNNGDIFVQ
                     KAPAATIQTLAIALKELLNAHEHPINIIGTRHGEKLYEALLSREEMIAAEDMGDYYRV
                     PPDLRDLNYGKYVEHGDRRISEVEDYNSHNTERLDVEGMKELLLKLPFIRALRSGEDY
                     ELDS"
     gene            847..>1338
                     /locus_tag="ACHOSC_RS25485"
                     /old_locus_tag="pgaptmp_005097"
     CDS             847..>1338
                     /locus_tag="ACHOSC_RS25485"
                     /old_locus_tag="pgaptmp_005097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001529290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HGU4550838.1"
                     /translation="MKILVTGAAGFIGRNLVFRLKEAGYNELIAIDRNSSLTDLEQGL
                     KQADFIFHLAGVNRPVKESEFEEGNCDLTQQIVDILKKNNKDTPIMLSSSIQAECDNA
                     YGKSKAVAEKIIQQYGETTNAKYYIYRLPNVFGKWCQPNYNSFIATFCHRIANDEAIT
                     INDP"
BASE COUNT          407 a          234 c          298 g          399 t
ORIGIN      
        1 ataattccgt tctaaatgca acgcgtggtg ccgattttct gtatcatgca gcagccctta
       61 aacaagttcc ttcatgtgaa tttcacccta tggaggcggt taagacaaat gttctgggta
      121 cggaaaatgt tctggaggct gctattgcga atgggattaa acgcgtggtg tgcttgagta
      181 ccgataaagc cgtttatcct atcaatgcaa tgggcatatc taaggcaatg atggaaaaag
      241 ttattgttgc aaaatcacgt aatcttgaca gttcaaaaac agttatctgt ggaactcgtt
      301 atggaaatgt aatggcttca cgtggatcgg tcatcccatt atttgttgat ctaatcaaag
      361 ctggtaaacc attgaccata accgatccca atatgactcg tttcatgatg acgcttgagg
      421 atgctgtcga tctggtcctt tatgctttcg aacatggaaa taatggtgac attttcgttc
      481 agaaagcacc tgcggcaaca attcaaacat tagccattgc acttaaggaa ttgctaaatg
      541 cccatgagca tccaatcaat attattggaa ctcgacacgg ggaaaaactt tacgaagcgt
      601 tattgagccg agaggaaatg attgcagcgg aagatatggg tgattattat cgtgttccac
      661 cagatctccg cgatttgaac tatggaaaat atgtggaaca tggtgaccgt cgtatctcgg
      721 aagtggaaga ttataattct cataatactg agagattaga tgttgaggga atgaaagaat
      781 tactgctaaa acttcctttt atccgggcac ttcgttctgg tgaagattat gagttggatt
      841 cataatatga aaattttagt tactggcgct gcagggttta tcggtcgaaa tttggttttc
      901 cgccttaagg aagctggata taacgaactt attgcgatag atcgtaactc ttctttgacg
      961 gatttagagc agggacttaa gcaggcagat ttcatttttc atcttgctgg agtaaatcgt
     1021 cctgtgaagg agagtgagtt tgaagagggg aattgcgatc taactcaaca gattgttgat
     1081 attctgaaaa aaaataataa agatactcct atcatgctta gttcatccat ccaagctgaa
     1141 tgtgataacg cttatggaaa gagtaaagca gttgcggaaa aaatcattca gcagtatggg
     1201 gaaacgacaa acgctaaata ttatatttat cgcttgccga atgtattcgg taagtggtgt
     1261 caaccaaatt ataactcctt tatagcaact ttctgccatc gcattgcaaa tgatgaagct
     1321 attacaatta atgatcct
//
LOCUS       DAZKHF020000523         1331 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2201S31241
            SAMN43267800-rid25106413.denovo.527, whole genome shotgun sequence.
ACCESSION   DAZKHF020000523 DAZKHF020000000
VERSION     DAZKHF020000523.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267800
            Sequence Read Archive: SRR30307263
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1331)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1331)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 1331)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350581.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350581.2
            Genome Coverage       :: 151x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:21:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,652
            CDSs (total)                      :: 5,557
            Genes (coding)                    :: 5,320
            CDSs (with protein)               :: 5,320
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 237
            CDSs (without protein)            :: 237
            Pseudo Genes (ambiguous residues) :: 0 of 237
            Pseudo Genes (frameshifted)       :: 102 of 237
            Pseudo Genes (incomplete)         :: 130 of 237
            Pseudo Genes (internal stop)      :: 52 of 237
            Pseudo Genes (multiple problems)  :: 41 of 237
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1331
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267800-rid25106413.denovo.527"
                     /serotype="O 26:H11"
                     /isolate="2201S31241"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-01"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            <1..908
                     /gene="espB"
                     /locus_tag="ACHOSC_RS25490"
                     /old_locus_tag="pgaptmp_005098"
     CDS             <1..908
                     /gene="espB"
                     /locus_tag="ACHOSC_RS25490"
                     /old_locus_tag="pgaptmp_005098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001092012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="type III secretion system LEE translocon
                     pore-forming subunit EspB"
                     /protein_id="HGU4550839.1"
                     /translation="NSVSENTTGSNAITASAINSSLLTDGKVDVSKLMLEIQKLLGKM
                     VRILQDYQQQQLSQSYQIQLAVFESQNKAIDEKKAAATAALVGGAISSVLGILGSFAA
                     INSATKGASDIAQKTASTSSKAIDAASDTATKTLTKATESVADAVEDASSVMQQAMTT
                     ATRAASRTSDVADDIADSAQRASQLAENAADAAQKASRASRFMAAVDKITGSTPFIAV
                     TSLAEGTKTLPTTVSESVKSNHEISEQRYKSVENFQQGNLDLYKQEVRRAQDDIASRL
                     RDMTTAARDLTDLQNRMGQSVRLAG"
     gene            915..1322
                     /gene="cesD2"
                     /locus_tag="ACHOSC_RS25495"
                     /old_locus_tag="pgaptmp_005099"
     CDS             915..1322
                     /gene="cesD2"
                     /locus_tag="ACHOSC_RS25495"
                     /old_locus_tag="pgaptmp_005099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LcrR family type III secretion system chaperone
                     CesD2"
                     /protein_id="HGU4550840.1"
                     /translation="MVDTFNDEVLNHYLEQKGYTIQKEFLCGSAFFIGWRIETSFFSL
                     AYRLDEQELILCSFEARNQTGLNGPVLSLTRLLEELYHHFSGIKKISAMKSKIGSDSE
                     RQKREELFNYFIRKGAVQQETEDGIWFVMNVNS"
BASE COUNT          376 a          274 c          321 g          360 t
ORIGIN      
        1 ttaattctgt ttcggagaat actaccggct ctaatgcaat taccgcatct gctattaatt
       61 catctttgct taccgatggt aaggtcgatg tttctaaact gatgctggaa attcaaaaac
      121 tcctgggcaa gatggtgcgt atattgcagg attaccaaca gcaacagttg tcgcagagct
      181 atcagatcca actggccgtt tttgagagcc agaataaagc cattgatgaa aaaaaggccg
      241 ctgcaacagc cgctctggtt ggtggggcta tttcatcagt attggggatc ttaggctctt
      301 ttgcagcaat taacagtgct acgaaaggcg cgagtgatat tgctcaaaaa accgcctcta
      361 catcttctaa ggctattgat gcggcttctg atactgcgac taaaacgttg actaaggcaa
      421 cggaaagcgt tgctgatgct gttgaagatg catccagcgt gatgcagcaa gcgatgacta
      481 cagcaacgag agcggccagc cgtacatccg acgttgctga tgacattgcc gattctgctc
      541 agagagcttc tcagctggct gaaaacgctg cagatgccgc tcagaaggca agtcgggcaa
      601 gccgctttat ggctgcagta gataagatta ctggctctac accatttatt gccgttacca
      661 gtcttgccga aggcacgaag acattgccaa caacggtatc tgaatcagtc aaatctaacc
      721 atgagattag cgaacagcgt tataagtctg tggagaactt ccagcagggt aatttggatc
      781 tgtataagca agaagttcgc agagcgcagg atgatatcgc tagccgtctg cgtgatatga
      841 caacagccgc tcgcgatctc actgatcttc agaatcgtat gggtcaatcg gttcgcttag
      901 ctgggtaatt gatcatggtc gatacgttta atgatgaagt gcttaatcac tatcttgaac
      961 aaaaagggta cacaatacag aaggagtttc tttgtggcag tgcctttttt atcggatggc
     1021 ggattgagac ctcttttttc tcattagcgt acagacttga tgaacaagaa ctgattttgt
     1081 gctcttttga agcacgtaac caaacagggc ttaacggccc tgttttatca ctgactcgct
     1141 tgctcgaaga gttgtaccac catttttcgg gtattaagaa aatcagtgcg atgaaatcta
     1201 agattggttc agattcagaa cgtcaaaagc gcgaagagtt gtttaattac ttcatcagaa
     1261 agggcgctgt gcagcaagaa acagaagacg gaatttggtt cgtaatgaat gtaaatagtt
     1321 agaaattttt a
//
LOCUS       DAZKHF020000524         1330 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2201S31241
            SAMN43267800-rid25106413.denovo.528, whole genome shotgun sequence.
ACCESSION   DAZKHF020000524 DAZKHF020000000
VERSION     DAZKHF020000524.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267800
            Sequence Read Archive: SRR30307263
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1330)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1330)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 1330)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350581.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350581.2
            Genome Coverage       :: 151x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:21:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,652
            CDSs (total)                      :: 5,557
            Genes (coding)                    :: 5,320
            CDSs (with protein)               :: 5,320
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 237
            CDSs (without protein)            :: 237
            Pseudo Genes (ambiguous residues) :: 0 of 237
            Pseudo Genes (frameshifted)       :: 102 of 237
            Pseudo Genes (incomplete)         :: 130 of 237
            Pseudo Genes (internal stop)      :: 52 of 237
            Pseudo Genes (multiple problems)  :: 41 of 237
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1330
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267800-rid25106413.denovo.528"
                     /serotype="O 26:H11"
                     /isolate="2201S31241"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-01"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            <1..63
                     /locus_tag="ACHOSC_RS25500"
                     /old_locus_tag="pgaptmp_005100"
     CDS             <1..63
                     /locus_tag="ACHOSC_RS25500"
                     /old_locus_tag="pgaptmp_005100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4550841.1"
                     /translation="KLGLHSIVELIDYAKLYELI"
     gene            complement(66..581)
                     /gene="sfmF"
                     /locus_tag="ACHOSC_RS25505"
                     /old_locus_tag="pgaptmp_005101"
     CDS             complement(66..581)
                     /gene="sfmF"
                     /locus_tag="ACHOSC_RS25505"
                     /old_locus_tag="pgaptmp_005101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbria assembly protein"
                     /protein_id="HGU4550842.1"
                     /translation="MRRVLFSCFCGLLWSSIGWAADPLGTININLHGNVVDFSCTVNT
                     ADIDKTVDLGRWPTTQLLNAGDTTALVPFSLRLEGCPPGSVAILFTGTPASDTNLLAL
                     DDPAMAQTVAIELRNSDRSRLALGEASPTEEVDANGNVTLNFFANYRALASGVRPGVA
                     KADAIFMINYN"
     gene            complement(592..>1330)
                     /gene="fimH"
                     /locus_tag="ACHOSC_RS25510"
                     /old_locus_tag="pgaptmp_005102"
     CDS             complement(592..>1330)
                     /gene="fimH"
                     /locus_tag="ACHOSC_RS25510"
                     /old_locus_tag="pgaptmp_005102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308622.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="type 1 fimbria D-mannose specific adhesin FimH"
                     /protein_id="HGU4550843.1"
                     /translation="TIDNYKYMQLHDYLLGAMSLVDSVMDIQFPPQNYIRMGTDPNVS
                     QNLPFGVMDSRLIFRLKVIRPFINMVEIPRQVMFTVYVTSTPYDPLVTPVYTISFGGR
                     VEVPQNCELNAGQIVEFDFGDIGASLFSAAGPGNRPAGVMPQTKSIAVKCTNVAAQAY
                     LTMRLEASAVSGQAMVSDNQDLGFIVADQNDTPITPNDLNSVIPFRLDAAAAANVTLR
                     AWPISITGQKPTEGPFSALGYLRVDYQ"
BASE COUNT          377 a          340 c          286 g          327 t
ORIGIN      
        1 aagctaggtt tgcattcaat tgtagagctt atcgactacg ccaaattata cgaattaata
       61 taatattaat tataattgat cataaatatc gcatccgctt tcgccacacc aggccgaaca
      121 ccgctggcta acgctcgata attggcaaaa aagtttagtg tgacattgcc atttgcatct
      181 acttcctcag tcgggctcgc ctcccccagt gcgagccggg agcgatcgct attacgtaat
      241 tcgatggcga cggtttgtgc cattgcggga tcatccagag ccagcaggtt ggtatcggat
      301 gccggcgttc ccgtaaataa aatcgcaact gaacccggag gacatccctc cagccgcagg
      361 ctaaaaggga cgagtgccgt ggtatcgcca gcgttcagta attgtgtcgt aggccatctg
      421 cctaaatcta ccgtcttatc aatatccgct gtgtttacgg tacaggagaa atcaacaacg
      481 ttaccgtgca aattgatatt aatcgttcct aaagggtcag ctgcccatcc aatggaactc
      541 cacagtaacc cgcagaaaca gctaaagagt actcttctca ttctccgtac ctcattgata
      601 atcgacgcgt aaatacccca gcgcgctaaa cggcccctcg gtcggttttt gaccggtaat
      661 actgataggc caggcgcgaa gtgtgacatt ggctgccgca gctgcatcca gacggaaagg
      721 aataacgcta ttgagatcgt taggcgtaat cggcgtatcg ttctgatcgg cgacaataaa
      781 acctaaatcc tgattgtccg acaccatcgc ctgaccagaa acggcactgg cttccagacg
      841 cattgttaaa taagcctgcg cagcaacatt cgtacatttg accgcaatgc tcttggtttg
      901 cggcatgacg ccagctggtc gattccccgg ccctgccgca ctaaataacg atgcgccgat
      961 atcaccaaaa tcaaattcaa caatctgccc ggcatttaat tcgcagtttt gcggcacttc
     1021 aacccggcca ccaaaactaa tggtataaac aggtgtaacc aacggatcgt aaggcgttga
     1081 tgtcacatac acggtaaaca tcacctgtct ggggatctcc accatgttaa taaagggacg
     1141 aataaccttt aaacgaaata ttaaacgaga atccatcacc ccgaatggaa ggttttgcga
     1201 aacgttagga tctgttccca tccgaatata attttgcggg gggaactgaa tatccatcac
     1261 actatcaacc agactcatcg cacctaatag ataatcatgt aattgcatat atttataatt
     1321 atcgatagtt
//
LOCUS       DAZKHF020000525         1328 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2201S31241
            SAMN43267800-rid25106413.denovo.529, whole genome shotgun sequence.
ACCESSION   DAZKHF020000525 DAZKHF020000000
VERSION     DAZKHF020000525.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267800
            Sequence Read Archive: SRR30307263
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1328)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1328)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 1328)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350581.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350581.2
            Genome Coverage       :: 151x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:21:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,652
            CDSs (total)                      :: 5,557
            Genes (coding)                    :: 5,320
            CDSs (with protein)               :: 5,320
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 237
            CDSs (without protein)            :: 237
            Pseudo Genes (ambiguous residues) :: 0 of 237
            Pseudo Genes (frameshifted)       :: 102 of 237
            Pseudo Genes (incomplete)         :: 130 of 237
            Pseudo Genes (internal stop)      :: 52 of 237
            Pseudo Genes (multiple problems)  :: 41 of 237
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1328
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267800-rid25106413.denovo.529"
                     /serotype="O 26:H11"
                     /isolate="2201S31241"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-01"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            28..432
                     /gene="stpA"
                     /locus_tag="ACHOSC_RS25515"
                     /old_locus_tag="pgaptmp_005103"
     CDS             28..432
                     /gene="stpA"
                     /locus_tag="ACHOSC_RS25515"
                     /old_locus_tag="pgaptmp_005103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein StpA"
                     /protein_id="HGU4550844.1"
                     /translation="MSVMLQSLNNIRTLRAMAREFSIDVLEEMLEKFRVVTKERREEE
                     EQQQRELAERQEKISTWLELMKADGINPEELLGNSSAAAPRAGKKRQPRPAKYKFTDV
                     NGETKTWTGQGRTPKPIAQALAEGKSLDDFLI"
     gene            complement(479..1003)
                     /gene="ygaP"
                     /locus_tag="ACHOSC_RS25520"
                     /old_locus_tag="pgaptmp_005104"
     CDS             complement(479..1003)
                     /gene="ygaP"
                     /locus_tag="ACHOSC_RS25520"
                     /old_locus_tag="pgaptmp_005104"
                     /EC_number="2.8.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001461399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiosulfate sulfurtransferase YgaP"
                     /protein_id="HGU4550845.1"
                     /translation="MALTTISPHDAQELIARGAKLIDIRDADEYLREHIPEADLAPLS
                     VLEQSGLPPKLRREQIIFHCQAGKRTSNNADKLAAIAAPAEIFLLEDGIDGWKRAGLP
                     VAVNKSQPLPLMRQVQIAAGGLILIGVVLGYTVNSGFFLLSGFVGAGLLFAGISGFCG
                     MARLLDKMPWNQRT"
     gene            complement(1013..1312)
                     /locus_tag="ACHOSC_RS25525"
                     /old_locus_tag="pgaptmp_005105"
     CDS             complement(1013..1312)
                     /locus_tag="ACHOSC_RS25525"
                     /old_locus_tag="pgaptmp_005105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HGU4550846.1"
                     /translation="MTELAQLQASAEQAAALLKAMSHPKRLLILCMLSGSPGTSAGEL
                     TRITGLSASATSQHLARMRDEGLIDSQRDAQRILYSIKNEAVNAIIATLKNVYCP"
BASE COUNT          349 a          317 c          320 g          342 t
ORIGIN      
        1 ttttggcgtt aaaaggtttt ctttattatg tccgtaatgt tacaaagttt aaataacatt
       61 cgcactctcc gtgcgatggc tcgcgaattc tccattgacg ttcttgaaga aatgctcgaa
      121 aaattcaggg ttgtcactaa agaaagacgt gaagaagaag aacagcagca gcgtgaactg
      181 gctgagcgtc aggaaaaaat tagcacctgg ctggagctga tgaaggctga cggaattaac
      241 ccggaagagt tattaggtaa tagctctgct gctgcaccac gcgctggtaa aaaacgccag
      301 ccgcgtccgg cgaaatataa attcaccgat gttaacggtg aaactaaaac ctggaccggt
      361 cagggccgta caccgaagcc aattgctcag gcgctggcag aaggtaaatc tctcgacgat
      421 ttcctgatct aattactgag gccggatgtc gctgctaggg cgcgtccggc gtcgcttctc
      481 aagttcgttg gttccacggc atcttatcta acaaccttgc cattccacaa aaaccgctga
      541 ttcctgcaaa cagtaacccg gcaccaacaa agccacttaa taagaagaaa ccgctattta
      601 cggtataacc cagtacaacg ccgattaata ttaaaccacc cgcagcgatc tgcacctggc
      661 gcattaacgg caagggttga gatttattta ccgctactgg caatccagct cttttccagc
      721 catcaatccc atcttcgagt aaaaatattt ctgcgggggc ggcaatcgct gctaatttat
      781 cggcgttatt actggtgcgt ttacctgcct ggcagtggaa tataatttgc tcgcgacgta
      841 atttaggtgg aagacctgac tgttccagca cggataatgg agccagatct gcttcaggaa
      901 tatgttcacg aagatattca tcagcgtcac gaatatcgat taactttgcg ccgcgtgcga
      961 ttaattcttg tgcatcatgc ggcgaaatgg ttgtcaaagc catagtgact ccttacggac
     1021 aatagacatt tttcagggtg gcgataatgg catttaccgc ctcattttta atggaatata
     1081 gaatgcgttg ggcatcccgt tggctgtcga taagcccttc gtcccgcata cgagcgagat
     1141 gctgtgatgt cgcagaggca ctcagtccgg taatgcgcgt cagctctccc gcgctggtgc
     1201 cgggggaacc gctaagcatg cacagaatca gcaatcgttt aggatggctc attgctttca
     1261 ataaggccgc tgcctgttcg gcactggcct gtaattgcgc gagttcagtc atatttattt
     1321 aagtattt
//
LOCUS       DAZKHF020000526         1317 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2201S31241
            SAMN43267800-rid25106413.denovo.530, whole genome shotgun sequence.
ACCESSION   DAZKHF020000526 DAZKHF020000000
VERSION     DAZKHF020000526.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267800
            Sequence Read Archive: SRR30307263
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1317)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1317)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 1317)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350581.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350581.2
            Genome Coverage       :: 151x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:21:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,652
            CDSs (total)                      :: 5,557
            Genes (coding)                    :: 5,320
            CDSs (with protein)               :: 5,320
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 237
            CDSs (without protein)            :: 237
            Pseudo Genes (ambiguous residues) :: 0 of 237
            Pseudo Genes (frameshifted)       :: 102 of 237
            Pseudo Genes (incomplete)         :: 130 of 237
            Pseudo Genes (internal stop)      :: 52 of 237
            Pseudo Genes (multiple problems)  :: 41 of 237
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1317
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267800-rid25106413.denovo.530"
                     /serotype="O 26:H11"
                     /isolate="2201S31241"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-01"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            <1..1246
                     /gene="frc"
                     /locus_tag="ACHOSC_RS25530"
                     /old_locus_tag="pgaptmp_005106"
     CDS             <1..1246
                     /gene="frc"
                     /locus_tag="ACHOSC_RS25530"
                     /old_locus_tag="pgaptmp_005106"
                     /EC_number="2.8.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033608 - formyl-CoA transferase activity
                     [Evidence IEA];
                     GO_process: GO:0033611 - oxalate catabolic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="formyl-CoA transferase"
                     /protein_id="HGU4550847.1"
                     /translation="TPLQGIKVLDFTGVQSGPSCTQMLAWFGADVIKIERPGVGDVTR
                     HQLRDIPDIDALYFTMLNSNKRSIELNTKTAEGKEVMEKLIREADILVENFHPGAIDH
                     MGFTWEHIQEINPRLIFGSIKGFDECSPYVNVKAYENVAQAAGGAASTTGFWDGPPLV
                     SAAALGDSNTGMHLLIGLLAALLHREKTGRGQRVTMSMQDAVLNLCRVKLRDQQRLDK
                     LGYLEEYPQYPNGTFGDAVPRGGNAGGGGQPGWILKCKGWETDPNAYIYFTIQEQNWE
                     NTCKAIGKPEWITDPAYSTAHARQPHIFDIFAEIEKYTVTIDKHEAVAYLTQFDIPCA
                     PVLSMKEISLDPSLRQSGSVVEVEQPLRGKYLTVGCPMKFSAFTPDIKAAPLLGEHTA
                     AVLQELGYSDDEIAAMKQNHAI"
BASE COUNT          353 a          288 c          324 g          352 t
ORIGIN      
        1 aactccactt caaggaatta aagttctcga tttcaccggt gtgcaatctg gcccatcttg
       61 tactcaaatg ctggcctggt ttggcgctga cgttattaaa attgaacgtc ccggcgttgg
      121 tgacgtaacg cgtcaccagc tgcgagatat tcctgatatc gatgcgcttt acttcaccat
      181 gcttaacagt aacaaacgtt ctattgagtt aaataccaaa acagcggaag gcaaagaggt
      241 aatggaaaag ctgatccgcg aagctgatat cttagtcgag aactttcatc caggggccat
      301 tgatcacatg ggcttcacct gggagcatat tcaagaaatc aatccacgtc tgatttttgg
      361 ttcgatcaaa gggtttgatg agtgttcgcc ttatgtgaat gtaaaagcct atgaaaacgt
      421 tgctcaggca gcgggtggcg cggcatccac tacgggtttt tgggatggtc cgccgctggt
      481 aagcgctgca gcgttgggtg acagcaacac cggaatgcat ttgctgatcg gtttacttgc
      541 tgctttgctg catcgcgaaa aaacggggcg tgggcaacga gtcaccatgt caatgcagga
      601 tgccgtattg aacctttgcc gcgtgaaatt acgtgaccag cagcgtctcg ataaattggg
      661 ttatctggaa gaatacccgc agtatccgaa tggtacattt ggtgatgcag ttccccgcgg
      721 tggtaatgca ggtggtggcg gtcagcctgg ctggatcctg aaatgtaaag gctgggaaac
      781 cgatcctaac gcctatattt atttcactat tcaggagcaa aactgggaaa acacctgtaa
      841 agccatcggc aaaccagaat ggattaccga tccggcatac agtacagccc atgcacgaca
      901 gccacatatt ttcgatattt ttgctgaaat cgaaaaatac actgtcacta ttgataaaca
      961 tgaagcggtg gcctatttga ctcagtttga tattccttgt gcaccggttt taagtatgaa
     1021 agaaatttca cttgatccct ctttgcgcca aagtggcagt gttgttgaag tggaacaacc
     1081 gttgcgtgga aaatatctga ccgttggttg tccaatgaaa ttctctgcct ttacgccgga
     1141 tattaaagct gcgccgctat taggtgaaca taccgctgct gtattgcagg agctgggtta
     1201 tagcgacgat gaaattgctg caatgaagca aaaccacgcc atctgatatt aacgggggct
     1261 tctggctccc aatttataaa aatttcgagg ttattaatca tgtcagatca acttcaa
//
LOCUS       DAZKHF020000527         1316 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2201S31241
            SAMN43267800-rid25106413.denovo.531, whole genome shotgun sequence.
ACCESSION   DAZKHF020000527 DAZKHF020000000
VERSION     DAZKHF020000527.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267800
            Sequence Read Archive: SRR30307263
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1316)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1316)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 1316)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350581.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350581.2
            Genome Coverage       :: 151x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:21:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,652
            CDSs (total)                      :: 5,557
            Genes (coding)                    :: 5,320
            CDSs (with protein)               :: 5,320
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 237
            CDSs (without protein)            :: 237
            Pseudo Genes (ambiguous residues) :: 0 of 237
            Pseudo Genes (frameshifted)       :: 102 of 237
            Pseudo Genes (incomplete)         :: 130 of 237
            Pseudo Genes (internal stop)      :: 52 of 237
            Pseudo Genes (multiple problems)  :: 41 of 237
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1316
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267800-rid25106413.denovo.531"
                     /serotype="O 26:H11"
                     /isolate="2201S31241"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-01"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            complement(<1..1181)
                     /locus_tag="ACHOSC_RS25535"
                     /old_locus_tag="pgaptmp_005107"
     CDS             complement(<1..1181)
                     /locus_tag="ACHOSC_RS25535"
                     /old_locus_tag="pgaptmp_005107"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0046797 - viral procapsid maturation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HK97 family phage prohead protease"
                     /protein_id="HGU4550848.1"
                     /translation="MTLKRACSLLTVKSFSEDERVITGIASTPSPDRDGDILEPEGAE
                     FGSTIPFLWQHDHSRPVGQCTVRRVREGLEITAMLVKPEPGMPSQMAARLDEAWAAIK
                     TGLVRGLSVGFRPHEYTYLDGGGLHFLRWELMEVSAVTVPANAECTIRTIKYFDRPFS
                     AASGNRKPVVKIASSAGASAQSITSFHKEKSAMNTGEQIKSFENKRAALAASLEEIMN
                     KAAEEGRTLDVEEEEHYDNTAAEIRQVDAHLKRLRELETSKAATAQPVKQAGNGNVAT
                     VASAPVIRVEQKLEKGIGFARFAKSLAAAKGVRSEALEVARRQYPDDSRLHHVLKSAV
                     GAGTTTDPQWAGSLSEYQEYAQDFIDYLRPQTIIGRFGQGGIPALRQVPFNIRVHAQV
                     SG"
BASE COUNT          276 a          409 c          347 g          284 t
ORIGIN      
        1 ccggacacct gggcatgcac gcggatattg aacggcacct gacgaagtgc agggatcccg
       61 ccctgaccaa atcgcccgat aatggtctgc ggacgcaggt aatcaataaa gtcctgcgca
      121 tattcctggt attcagacag gctgcctgcc cactgcgggt ccgtggtggt ccctgccccc
      181 accgccgatt tcaggacatg atgcagacgg ctgtcatccg gatactgacg acgggccact
      241 tccagggctt cagagcggac acctttagcc gcggccagtg atttggcaaa gcgggcgaaa
      301 ccaatcccct tctccagttt ctgctcaaca cggatcaccg gcgctgaagc caccgtggcc
      361 acattcccgt taccggcctg tttcaccggc tgtgccgtgg cggccttact ggtttccagt
      421 tcacgcagac gcttcaggtg cgcatccacc tgacggattt ccgctgcggt gttgtcgtag
      481 tgctcttcct cttccacatc cagtgtgcgg ccttcctctg cggctttgtt catgatctcc
      541 tcaagggagg ctgccagcgc cgcacgcttg ttttcaaaac ttttaatctg ttcaccagta
      601 ttcattgctg acttttcctt atgaaaagag gttattgact gtgccgaagc gccggcagaa
      661 gatgcgattt tcaccaccgg tttccggttg ccggacgcgg cagaaaacgg gcggtcgaaa
      721 tatttaatgg tccggatggt gcattccgca ttcgcgggca cggtgacggc agacacctcc
      781 atcagctccc agcgcagaaa atgcagtccg cctccgtcca gataagtgta ttcatggggc
      841 cggaagccca cagaaagccc cctgaccagc ccggtcttaa tggcagccca ggcctcatcc
      901 agccgggctg ccatctggga gggcatcccc ggctccggct tcaccagcat tgccgtgatt
      961 tccagccctt ccctgacccg acgcaccgta cactggccta cagggcggga atggtcatgc
     1021 tgccagagaa acgggatcgt actgccaaac tccgccccct ccggctccag gatgtcacca
     1081 tcccgatccg gagaaggcgt tgacgcaatc ccggtgatca cccgttcatc ctcactgaag
     1141 gatttcaccg tcagcaggga acaggcccgt ttaagagtca catcagcctc ctgaaaataa
     1201 aaaaaccgcc gcagcggttc atgatggtta cagggtgagc agggttatat gaaaaaaacc
     1261 tcatacgctt tctttttcgg ttccggattc agggacatca gggacaccgc attgaa
//
LOCUS       DAZKHF020000528         1315 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2201S31241
            SAMN43267800-rid25106413.denovo.532, whole genome shotgun sequence.
ACCESSION   DAZKHF020000528 DAZKHF020000000
VERSION     DAZKHF020000528.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267800
            Sequence Read Archive: SRR30307263
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1315)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1315)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 1315)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350581.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350581.2
            Genome Coverage       :: 151x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:21:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,652
            CDSs (total)                      :: 5,557
            Genes (coding)                    :: 5,320
            CDSs (with protein)               :: 5,320
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 237
            CDSs (without protein)            :: 237
            Pseudo Genes (ambiguous residues) :: 0 of 237
            Pseudo Genes (frameshifted)       :: 102 of 237
            Pseudo Genes (incomplete)         :: 130 of 237
            Pseudo Genes (internal stop)      :: 52 of 237
            Pseudo Genes (multiple problems)  :: 41 of 237
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1315
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267800-rid25106413.denovo.532"
                     /serotype="O 26:H11"
                     /isolate="2201S31241"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-01"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            <1..279
                     /locus_tag="ACHOSC_RS25540"
                     /old_locus_tag="pgaptmp_005108"
     CDS             <1..279
                     /locus_tag="ACHOSC_RS25540"
                     /old_locus_tag="pgaptmp_005108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2511 domain-containing protein"
                     /protein_id="HGU4550849.1"
                     /translation="PFKTIERESFNGVWPFNTDEVQLQCLDGNPYVMNFDDNKLYALT
                     GLARIKGKTFGALPLDNNNPFWLDNDAAPGLKKSLGDVTKAAFDLCDK"
     gene            285..1286
                     /locus_tag="ACHOSC_RS25545"
                     /old_locus_tag="pgaptmp_005109"
     CDS             285..1286
                     /locus_tag="ACHOSC_RS25545"
                     /old_locus_tag="pgaptmp_005109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HGU4550850.1"
                     /translation="MSVRKIPSGKWLCECYPYGASGKRIRKQFATKSEALSYERRLMN
                     SRVGDEFQDGSGPRLSELIARWFEMYGKTLSSGAERKVKLEAICSRLGDPFASQFDKN
                     MFATYRERRLSGEWNPKGKKKLSEATVNREQSYLHAVFAELKRLGEWSGENPLTGIRK
                     FREEEKELAFLYVDEIERLLIACDESRNKDLGVVVRIGLATGARWSEAEGLKQSQVLP
                     GRITFVKTKGKKNRTVPISPQLQAMLPQKRGALFSPCYEAFDAAIKRAKIELPDGQLT
                     HVLRHTFASHFMMRGGNILVLQKILGHSDIKMTMRYAHFAPGHLEAAVELNPFDNRG"
BASE COUNT          371 a          225 c          339 g          380 t
ORIGIN      
        1 ccttttaaaa ctattgaacg tgagagtttc aatggggtat ggccatttaa tactgatgag
       61 gttcaattac agtgtcttga tggtaatcct tatgtgatga attttgacga taataagtta
      121 tatgcgctta caggtttagc tcgaataaaa ggtaaaacat ttggtgcgtt accgttagat
      181 aacaataatc cattttggct agataatgat gccgccccag ggttaaaaaa gagtctgggg
      241 gatgtcacta aggctgcatt tgatttatgt gataagtaac taaaatgtcg gttcgtaaga
      301 ttccatcagg taaatggctt tgcgaatgtt atccctacgg ggcatcggga aaacgcattc
      361 gtaaacagtt tgcgacaaaa agtgaggcgc tctcttatga gcgccgttta atgaatagta
      421 gagttggaga cgagtttcaa gatggttctg gtcctcgtct ttctgagttg attgctcgtt
      481 ggtttgagat gtacggtaaa accttgtcct ctggtgcaga gcgcaaagtc aaacttgagg
      541 cgatttgttc caggctgggg gatccatttg cttctcagtt tgacaaaaat atgtttgcta
      601 cttatcggga aagaaggcta tcaggagaat ggaatcccaa ggggaagaaa aaacttagtg
      661 aagcaaccgt taatcgcgag cagtcatatc tacatgctgt ttttgccgaa ctgaagcgcc
      721 ttggggagtg gtctggtgaa aaccccctga ctggtattcg caagtttcgt gaggaagaaa
      781 aggaactggc gtttctgtat gtagatgaga ttgaacgcct tctgattgcg tgtgatgagt
      841 cacggaataa agatttgggg gttgttgtcc gtattgggct tgcgactggt gctcggtgga
      901 gtgaagctga aggattaaag caatctcaag tactgcccgg tcgaatcaca tttgttaaaa
      961 ctaaaggaaa gaagaaccgc actgtaccga tttcacctca attgcaggct atgcttcctc
     1021 aaaaacgagg agcgctattt tcaccatgtt atgaggcttt tgacgctgca attaagagag
     1081 cgaagatcga gcttcctgat gggcaattaa ctcatgtgct acgtcacacg tttgccagtc
     1141 attttatgat gcggggcgga aatattcttg tgttgcaaaa aatactgggg catagcgata
     1201 taaaaatgac tatgcgttat gcgcattttg ctccaggtca tttagaggct gctgttgaat
     1261 tgaacccttt tgacaataga gggtaaaaag tggcgataaa aagtgacaat agatt
//
LOCUS       DAZKHF020000529         1312 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2201S31241
            SAMN43267800-rid25106413.denovo.533, whole genome shotgun sequence.
ACCESSION   DAZKHF020000529 DAZKHF020000000
VERSION     DAZKHF020000529.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267800
            Sequence Read Archive: SRR30307263
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1312)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1312)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 1312)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350581.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350581.2
            Genome Coverage       :: 151x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:21:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,652
            CDSs (total)                      :: 5,557
            Genes (coding)                    :: 5,320
            CDSs (with protein)               :: 5,320
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 237
            CDSs (without protein)            :: 237
            Pseudo Genes (ambiguous residues) :: 0 of 237
            Pseudo Genes (frameshifted)       :: 102 of 237
            Pseudo Genes (incomplete)         :: 130 of 237
            Pseudo Genes (internal stop)      :: 52 of 237
            Pseudo Genes (multiple problems)  :: 41 of 237
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1312
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267800-rid25106413.denovo.533"
                     /serotype="O 26:H11"
                     /isolate="2201S31241"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-01"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            complement(<1..>1312)
                     /gene="ehxB"
                     /locus_tag="ACHOSC_RS25550"
                     /old_locus_tag="pgaptmp_005110"
                     /gene_synonym="hlyB"
     CDS             complement(<1..>1312)
                     /gene="ehxB"
                     /locus_tag="ACHOSC_RS25550"
                     /old_locus_tag="pgaptmp_005110"
                     /gene_synonym="hlyB"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="enterohemolysin T1SS ABC transporter
                     permease/ATPase EhxB"
                     /protein_id="HGU4550851.1"
                     /translation="LTGARTYIFSHTTSRIDVELSAKLFRHLLALPVSYFENRRVGET
                     VARVRELEQIRNFLTGQALTSVLDLFFSVIFFCVMWYYSPQLTLVILLSLPCYVIWSL
                     FISPLLRRRLDDKFLRNAENQAFLVETVTAINTIKSMAVSPQMIATWDKQLAGYVASS
                     FRVNLVAMTGQQGIQLIQKSVMVISLWMGAHLVISGEISIGQLIAFNMLAGQVIAPVI
                     RLAHLWQDFQQVGISVERLGDVLNTPVEKKSGRNILPEIQGDIEFKNVRFRYSSDGNV
                     ILNNINLYISKGDVIGIVGRSGSGKSTLTKLLQRFYIPETGQILIDGHDLSLADPEWL
                     RRQIGVVLQENILLNRSIIDNITLASPAVSMEQAIEAARLAGAHDFITELKEGYNTIV
                     GEQGVGLSGGQRQRIAIARALVTNPRILIFDEATSALDYESENI"
BASE COUNT          415 a          296 c          224 g          377 t
ORIGIN      
        1 attatatttt ccgactcata atcaagagca ctggttgctt catcaaaaat aagaattcga
       61 ggatttgtaa caagagcccg ggctattgcg atccgttggc gctgccctcc tgaaagacca
      121 acgccctgtt ctccaacaat agtattgtac ccttctttta gttctgtaat aaaatcatgg
      181 gcacctgcaa gtctggctgc ctcaatagcc tgttccatag atacagccgg agaagctaat
      241 gtaatattat cgataatact acgatttaat agtatatttt cctgcaatac aacaccaatc
      301 tggcgtcgta accattctgg atctgcaagt gataaatcat gcccatcaat taaaatctgt
      361 ccggtctctg gtatataaaa gcgctgaagc agttttgtta atgtactttt tcctgaacca
      421 gaacggccaa ctataccgat aacatccccc tttgatatgt ataaattaat attattcaaa
      481 ataacattac cgtcagaaga ataccgaaat ctgacatttt taaattcgat atccccctga
      541 atttccggca gtatatttct gcctgacttt ttctctaccg gtgtatttaa tacatcacca
      601 aggcgctcaa cagaaatacc aacctgctga aaatcctgcc aaagatgagc cagtctgata
      661 acaggagcaa tgacctgacc tgcaagcata ttaaacgcga ttaactgccc gatacttatc
      721 tctcccgata tgacaagatg tgctcccatc catagactaa ttaccattac acttttctgt
      781 atcagctgta tcccctgctg ccctgtcatt gcaaccagat ttaccctgaa actggaagca
      841 acatacccgg ccagttgttt atcccatgta gcaatcatct gtggtgatac tgccatggat
      901 ttaattgtat ttattgctgt taccgtttcg acaagaaaag cttgattttc tgcattcctg
      961 agaaacttat catcaagacg tcgacgtaat aagggtgata taaacaatga ccatataaca
     1021 taacaaggta gtgacaataa tataaccagt gttaattgag ggctgtaata ccacatgaca
     1081 caaaaaaata ttacagaaaa aaatagatca agaactgatg tcaacgcttg tccggttaaa
     1141 aaattacgaa tttgctccag ttcccttact ctggcaacgg tctctccgac cctgcgattt
     1201 tcaaaatatg aaacaggcaa tgcaagcaaa tgtctgaata acttagcgct cagttcgacg
     1261 tcaatacgac ttgtagtatg agagaaaata taagttctgg ctccggttaa ta
//
LOCUS       DAZKHF020000530         1311 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2201S31241
            SAMN43267800-rid25106413.denovo.534, whole genome shotgun sequence.
ACCESSION   DAZKHF020000530 DAZKHF020000000
VERSION     DAZKHF020000530.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267800
            Sequence Read Archive: SRR30307263
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1311)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1311)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 1311)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350581.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350581.2
            Genome Coverage       :: 151x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:21:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,652
            CDSs (total)                      :: 5,557
            Genes (coding)                    :: 5,320
            CDSs (with protein)               :: 5,320
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 237
            CDSs (without protein)            :: 237
            Pseudo Genes (ambiguous residues) :: 0 of 237
            Pseudo Genes (frameshifted)       :: 102 of 237
            Pseudo Genes (incomplete)         :: 130 of 237
            Pseudo Genes (internal stop)      :: 52 of 237
            Pseudo Genes (multiple problems)  :: 41 of 237
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1311
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267800-rid25106413.denovo.534"
                     /serotype="O 26:H11"
                     /isolate="2201S31241"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-01"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            84..1250
                     /gene="ugd"
                     /locus_tag="ACHOSC_RS25555"
                     /old_locus_tag="pgaptmp_005111"
     CDS             84..1250
                     /gene="ugd"
                     /locus_tag="ACHOSC_RS25555"
                     /old_locus_tag="pgaptmp_005111"
                     /EC_number="1.1.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003979 - UDP-glucose 6-dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0000271 - polysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose 6-dehydrogenase"
                     /protein_id="HGU4550852.1"
                     /translation="MKITISGTGYVGLSNGLLIAQNHEVVALDILPSRVAMLNDRISP
                     IVDKEIQQFLQSDKIHFNATLDKNEAYRDADYVIIATPTDYDPNTNYFNTSSVESVIK
                     DVVEINPYAVMVIKSTVPVGFTAAMHKKYRTKNIIFSPEFLREGKALYDNLHPSRIVI
                     GERSERAERFAALLQEGAIKQNIPTLFTDSTEAEAIKLFANTYLAMRVAYFNELDSYA
                     ESLGLNTRQIIEGVCLDPRIGNHYNNPSFGYGGYCLPKDTKQLLANYQSVPNNLISAI
                     VDANRTRKDFIADAILSRKPHVVGIYRLIMKSGSDNFRASSIQGIMKRIKAKGVEVII
                     YEPVMKEDSFFNSRLERDLATFKQQADVIISNRMAEELKDVADKVYTRDLFGSD"
BASE COUNT          374 a          286 c          275 g          376 t
ORIGIN      
        1 caacgtagcg ttaaaacttt tagctcttat caggatgcta aaaacatcat gattcacagt
       61 taagttaatt ctgagagcat gaaatgaaaa tcaccatttc cggtaccggc tatgtaggct
      121 tgtcaaacgg gcttctaatc gcacaaaatc atgaggttgt ggcattagat attttaccgt
      181 cacgcgttgc tatgctgaat gatcggatat ctcctattgt tgataaggaa attcagcagt
      241 ttttgcaatc agataaaata cactttaatg ccacattaga taaaaatgaa gcctaccggg
      301 atgctgatta tgtcatcatc gccactccaa ccgactatga tcctaacact aattatttca
      361 atacatcgag tgtagaatca gtaattaagg acgtagtaga gataaatcct tatgcggtta
      421 tggtcatcaa atcaacggta cccgttggct ttaccgcagc gatgcataag aaatatcgta
      481 ctaaaaatat tattttctct ccggaatttc tccgtgaggg taaagccctt tacgataatc
      541 tccatccttc acgtattgtc atcggtgagc gttcagaacg cgcagaacgt tttgctgctc
      601 tgttacagga aggcgcgatt aagcaaaata tcccgaccct gtttaccgac tcaaccgaag
      661 cagaagcgat taaacttttc gctaatacct atctggcgat gcgcgtagcg tactttaatg
      721 aactggatag ctacgcagaa agtttaggtc tgaatactcg ccagattatc gaaggcgttt
      781 gtctcgatcc gcgtattggc aaccattaca acaatccgtc gtttggttat ggtggttatt
      841 gtctgccgaa agataccaag cagttactgg cgaactatca gtctgtgccg aataacctga
      901 tctcggcaat tgtcgatgca aaccgcacgc gtaaagattt tattgccgat gccattttgt
      961 cacgcaaacc gcatgtggtg ggtatttatc gcctgattat gaagagcggt tcagataact
     1021 tccgtgcgtc ttctattcag gggattatga agcgtatcaa ggcgaaaggt gttgaagtaa
     1081 tcatctacga gccagtgatg aaagaagact cattcttcaa ctctcggctg gaacgtgatc
     1141 tcgccacttt caaacaacaa gccgacgtaa ttatctccaa ccgtatggca gaagagctta
     1201 aggatgtggc agacaaagtc tacacccgcg atctctttgg cagtgactaa catcctgtta
     1261 tcagggcgat tttcgccctg attctcttat gttccctttg taataattca t
//
LOCUS       DAZKHF020000531         1298 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2201S31241
            SAMN43267800-rid25106413.denovo.535, whole genome shotgun sequence.
ACCESSION   DAZKHF020000531 DAZKHF020000000
VERSION     DAZKHF020000531.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267800
            Sequence Read Archive: SRR30307263
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1298)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1298)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 1298)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350581.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350581.2
            Genome Coverage       :: 151x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:21:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,652
            CDSs (total)                      :: 5,557
            Genes (coding)                    :: 5,320
            CDSs (with protein)               :: 5,320
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 237
            CDSs (without protein)            :: 237
            Pseudo Genes (ambiguous residues) :: 0 of 237
            Pseudo Genes (frameshifted)       :: 102 of 237
            Pseudo Genes (incomplete)         :: 130 of 237
            Pseudo Genes (internal stop)      :: 52 of 237
            Pseudo Genes (multiple problems)  :: 41 of 237
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1298
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267800-rid25106413.denovo.535"
                     /serotype="O 26:H11"
                     /isolate="2201S31241"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-01"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            <1..456
                     /locus_tag="ACHOSC_RS25560"
                     /old_locus_tag="pgaptmp_005112"
     CDS             <1..456
                     /locus_tag="ACHOSC_RS25560"
                     /old_locus_tag="pgaptmp_005112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage antirepressor KilAC domain-containing
                     protein"
                     /protein_id="HGU4550853.1"
                     /translation="LVDRWRELEETAVNIPKTLPEALRLAADLAEQKMQLENQLAIAA
                     PKVEFADRVGEASGILIGNFAKVVGIGPNKLFAWMRDHKILIASGSRRNVPMQEYMDR
                     GYFTVKETAVNTNHGIQISFTTKITGRGQQWLTRKLLDNGMLKVTGEAA"
     gene            456..746
                     /locus_tag="ACHOSC_RS25565"
                     /old_locus_tag="pgaptmp_005113"
     CDS             456..746
                     /locus_tag="ACHOSC_RS25565"
                     /old_locus_tag="pgaptmp_005113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000002235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1364 domain-containing protein"
                     /protein_id="HGU4550854.1"
                     /translation="MANLRKESRGRECQVRIYGICNGNPETTVLAHYRMAGICGTGMK
                     PDDLIGAWACSACHDEIDRRTHNLDNKDARLYHLEGVIRTQAILLKEGKIKS"
     gene            743..1105
                     /locus_tag="ACHOSC_RS25570"
                     /old_locus_tag="pgaptmp_005114"
     CDS             743..1105
                     /locus_tag="ACHOSC_RS25570"
                     /old_locus_tag="pgaptmp_005114"
                     /EC_number="3.1.21.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001008185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RusA family crossover junction
                     endodeoxyribonuclease"
                     /protein_id="HGU4550855.1"
                     /translation="MNEYQFVLPYPPSVNTYWRRRGSQYYISDKGQKYRKDVQQIIHQ
                     LKLDIFTKSRLRIKVIADVPDSRRRDLDNILKGLLDSLIHAGFAEDDEQFDDIRVIRG
                     VKVPGGRLGIKITELENA"
     gene            1102..1290
                     /locus_tag="ACHOSC_RS25575"
                     /old_locus_tag="pgaptmp_005115"
     CDS             1102..1290
                     /locus_tag="ACHOSC_RS25575"
                     /old_locus_tag="pgaptmp_005115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000994505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein ninH"
                     /protein_id="HGU4550856.1"
                     /translation="MNATIQTIPELLIQTRGNQTEVARMLSCARGTVLKYNRDSKGER
                     HVIVNGVLMVKQGKRGRR"
BASE COUNT          389 a          312 c          335 g          262 t
ORIGIN      
        1 cttgttgacc gttggcgaga gcttgaagaa actgcggtta atatccccaa aacgttaccg
       61 gaagcgttgc gccttgctgc tgatcttgct gagcagaaaa tgcaactgga aaaccagctc
      121 gcaattgccg cacctaaagt tgagtttgcc gatcgcgttg gcgaggccag cggaattttg
      181 attggaaact ttgcaaaggt tgttggtatt ggtccaaaca aactgtttgc gtggatgcgc
      241 gatcacaaaa tccttattgc ttcaggttcc cggcgcaatg tgccaatgca ggaatatatg
      301 gatcgcggct atttcacagt gaaagaaaca gcggtcaaca caaatcacgg aatacagata
      361 tcgttcacca caaaaatcac cgggcgtggt caacagtggc tgaccagaaa gctgctcgat
      421 aacggaatgc tgaaagtaac aggggaggct gcttaatggc taacctacgc aaagaatctc
      481 gcggcagaga atgccaggta cgtatttacg gcatatgcaa tggcaatcct gaaactacag
      541 ttctggcaca ttaccggatg gctggaattt gcggaacggg aatgaagcct gacgacctga
      601 tcggcgcatg ggcttgtagc gcgtgtcacg atgaaatcga ccgacgcacc cataacctcg
      661 acaacaaaga cgccagactt taccacctcg aaggcgtgat caggacgcag gcgatactgc
      721 tgaaggaggg gaagattaag tcatgaacga atatcagttt gtgcttccat acccgccgtc
      781 ggtgaatacc tactggcgaa gacggggaag ccaatactac atcagcgata aaggccagaa
      841 ataccgaaaa gacgttcagc aaatcatcca ccaactcaag ttagatattt tcaccaaatc
      901 acgactccgc atcaaagtaa tcgcagacgt tccagactcc cgccgccgcg acctagataa
      961 catcctgaag ggtttactcg actcccttat ccatgccgga tttgcggaag acgacgagca
     1021 attcgatgac attcgcgtaa ttcgtggtgt gaaagtacca ggcggacggc ttggaataaa
     1081 aatcaccgaa ctggagaacg catgaacgcc acaattcaaa cgataccaga gcttcttatc
     1141 cagacacgag gcaatcagac cgaagtggcg aggatgcttt cctgcgcaag aggaacagtg
     1201 ctcaagtaca accgagacag caaaggcgag cgtcatgtaa tagttaacgg cgtcctgatg
     1261 gtcaaacagg gcaagagggg aagacgatga gactcgaa
//
LOCUS       DAZKHF020000532         1294 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2201S31241
            SAMN43267800-rid25106413.denovo.536, whole genome shotgun sequence.
ACCESSION   DAZKHF020000532 DAZKHF020000000
VERSION     DAZKHF020000532.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267800
            Sequence Read Archive: SRR30307263
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1294)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1294)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 1294)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350581.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350581.2
            Genome Coverage       :: 151x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:21:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,652
            CDSs (total)                      :: 5,557
            Genes (coding)                    :: 5,320
            CDSs (with protein)               :: 5,320
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 237
            CDSs (without protein)            :: 237
            Pseudo Genes (ambiguous residues) :: 0 of 237
            Pseudo Genes (frameshifted)       :: 102 of 237
            Pseudo Genes (incomplete)         :: 130 of 237
            Pseudo Genes (internal stop)      :: 52 of 237
            Pseudo Genes (multiple problems)  :: 41 of 237
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1294
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267800-rid25106413.denovo.536"
                     /serotype="O 26:H11"
                     /isolate="2201S31241"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-01"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            38..430
                     /gene="ydeI"
                     /locus_tag="ACHOSC_RS25580"
                     /old_locus_tag="pgaptmp_005116"
     CDS             38..430
                     /gene="ydeI"
                     /locus_tag="ACHOSC_RS25580"
                     /old_locus_tag="pgaptmp_005116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdeI family stress tolerance OB fold protein"
                     /protein_id="HGU4550857.1"
                     /translation="MKFQAIVLASFLVMPYALADDQGGLKQDAAPPPPHAIEDGYRGT
                     DDAKKMTVDFAKNMHDGASVSLRGNLISHKGEDRYVFRDKSGEINVVIPAAVFDGREV
                     QPDQMINISGSLDKKSAPAVVRVTHLQK"
     gene            685..>1294
                     /gene="dgcZ"
                     /locus_tag="ACHOSC_RS25585"
                     /old_locus_tag="pgaptmp_005117"
     CDS             685..>1294
                     /gene="dgcZ"
                     /locus_tag="ACHOSC_RS25585"
                     /old_locus_tag="pgaptmp_005117"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase DgcZ"
                     /protein_id="HGU4550858.1"
                     /translation="MIKKTTEIDAILLNLNKAIDAHYQWLVSMFHSVVARDASKPEIT
                     DNHSYGLCQFGRWIDHLGPLDNDELPYVRLMDSAHQHMHNCGRELMLAIVENHWQDAH
                     FDSFQEGLLSFTAALTDYKIYLLTIRSNMDVLTGLPGRRVLDESFDHQLRNTEPLNLY
                     LMLLDIDRFKLVNDTYGHLIGDVVLRTLATYLASWTRDYETVY"
BASE COUNT          356 a          253 c          305 g          380 t
ORIGIN      
        1 cttaataagt caaaccaaac gcaattaagg agttagtatg aaatttcagg ccattgtatt
       61 agcaagtttt cttgtcatgc cttatgcgtt ggcagatgac cagggcggtt taaaacagga
      121 tgcagcgcca ccgccgcctc atgcaataga agatggctat cgcggaaccg atgatgcaaa
      181 aaaaatgacc gttgatttcg caaaaaacat gcacgatggc gcttcggttt cactacgcgg
      241 taatttgatt tctcacaaag gagaggaccg ttacgttttt cgcgataaga gcggtgaaat
      301 taatgtcgtt attcctgcgg ccgtctttga tggacgagaa gttcagccgg accagatgat
      361 caacattagc ggcagtctgg ataagaaaag cgcgccggcc gtcgttcggg tcactcattt
      421 gcagaaataa aaataaatta gcctgatggc ctgaactact gggccattag ggtattgggt
      481 tatgctattt gtcatccttt tatttacgat aatgcagagg tgatttacag aattaaacac
      541 ttttttatcc gctgattttg ttttaaatca caatgttatc tcagagatga ctcataactt
      601 cggatttcta tgaaaatatt gacgtaattt tgcttttctc tcgttagaat agcgcgcaca
      661 aggaactgtg aaaaaggagt ggcaatgatc aagaaaacaa cggaaattga tgccatcttg
      721 ttaaatctca ataaggctat cgatgcccac taccagtggc tggtgagtat gtttcacagc
      781 gtggtcgcga gagatgccag taagccagaa ataacggata accattctta tggactgtgc
      841 cagtttggtc ggtggattga tcatctgggg ccactcgata acgatgaatt accttacgtt
      901 cggctaatgg attctgccca tcaacatatg cataactgtg gtcgggaatt aatgctggct
      961 attgttgaaa atcactggca ggacgcgcat ttcgactcct ttcaggaggg gttgctttct
     1021 tttactgcgg cattaaccga ttacaaaatt tatttgctga cgatccgtag caatatggat
     1081 gttttgacgg gattgccggg gcgtcgggtt cttgatgaat cctttgatca tcagttacgc
     1141 aacactgagc ctctgaatct ttatttaatg ttgttggata ttgaccgatt taaattggtt
     1201 aatgatacct acgggcattt aatcggcgat gtagtattac gcaccctggc aacttactta
     1261 gccagttgga cgcgtgatta cgaaacggtt tatc
//
LOCUS       DAZKHF020000533         1282 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2201S31241
            SAMN43267800-rid25106413.denovo.537, whole genome shotgun sequence.
ACCESSION   DAZKHF020000533 DAZKHF020000000
VERSION     DAZKHF020000533.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267800
            Sequence Read Archive: SRR30307263
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1282)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1282)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 1282)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350581.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350581.2
            Genome Coverage       :: 151x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:21:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,652
            CDSs (total)                      :: 5,557
            Genes (coding)                    :: 5,320
            CDSs (with protein)               :: 5,320
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 237
            CDSs (without protein)            :: 237
            Pseudo Genes (ambiguous residues) :: 0 of 237
            Pseudo Genes (frameshifted)       :: 102 of 237
            Pseudo Genes (incomplete)         :: 130 of 237
            Pseudo Genes (internal stop)      :: 52 of 237
            Pseudo Genes (multiple problems)  :: 41 of 237
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1282
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267800-rid25106413.denovo.537"
                     /serotype="O 26:H11"
                     /isolate="2201S31241"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-01"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            222..1169
                     /gene="stxA1a"
                     /locus_tag="ACHOSC_RS25590"
                     /old_locus_tag="pgaptmp_005118"
     CDS             222..1169
                     /gene="stxA1a"
                     /locus_tag="ACHOSC_RS25590"
                     /old_locus_tag="pgaptmp_005118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Shiga toxin Stx1a subunit A"
                     /protein_id="HGU4550859.1"
                     /translation="MKIIIFRVLTFFFVIFSVNVVAKEFTLDFSTAKTYVDSLNVIRS
                     AIGTPLQTISSGGTSLLMIDSGSGDNLFAVDVRGIDPEEGRFNNLRLIVERNNLYVTG
                     FVNRTNNVFYRFADFSHVTFPGTTAVTLSGDSSYTTLQRVAGISRTGMQINRHSLTTS
                     YLDLMSHSGTSLTQSVARAMLRFVTVTAEALRFRQIQRGFRTTLDDLSGRSYVMTAED
                     VDLTLNWGRLSSVLPDYHGQDSVRVGRISFGSINAILGSVALILNCHHHASRVARMAS
                     DEFPSMCPADGRVRGITHNKILWDSSTLGAILMRRTISS"
     gene            1179..>1282
                     /locus_tag="ACHOSC_RS25595"
                     /old_locus_tag="pgaptmp_005119"
     CDS             1179..>1282
                     /locus_tag="ACHOSC_RS25595"
                     /old_locus_tag="pgaptmp_005119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005576 - extracellular region [Evidence
                     IEA];
                     GO_process: GO:0019836 - hemolysis by symbiont of host
                     erythrocytes [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Shiga-like toxin beta subunit"
                     /protein_id="HGU4550860.1"
                     /translation="MKKTLLIAASLSFFSASALATPDCVTGKVEYTKY"
BASE COUNT          341 a          215 c          312 g          414 t
ORIGIN      
        1 gtaacatcct gtgtatcaat aaatgttgtt atctacgtac gtcaagtagt cgcatgagat
       61 ctgaccagat atgttaaggt tgcagctctc tttgaatatg attatcattt tcattacgtt
      121 attgttacgt ttatccggtg cgccgtaaaa cgccgtcctt cagggcgtgg aggatgtcaa
      181 gaatatagtt atcgtatggt gctcaaggag tattgtgtaa tatgaaaata attattttta
      241 gagtgctaac ttttttcttt gttatctttt cagttaatgt ggtggcgaag gaatttacct
      301 tagacttctc gactgcaaag acgtatgtag attcgctgaa tgtcattcgc tctgcaatag
      361 gtactccatt acagactatt tcatcaggag gtacgtcttt actgatgatt gatagtggct
      421 caggggataa tttgtttgca gttgatgtca gagggataga tccagaggaa gggcggttta
      481 ataatctacg gcttattgtt gaacgaaata atttatatgt gacaggattt gttaacagga
      541 caaataatgt tttttatcgc tttgctgatt tttcacatgt tacctttcca ggtacaacag
      601 cggttacatt gtctggtgac agtagctata ccacgttaca gcgtgttgca gggatcagtc
      661 gtacggggat gcagataaat cgccattcgt tgactacttc ttatctggat ttaatgtcgc
      721 atagtggaac ctcactgacg cagtctgtgg caagagcgat gttacggttt gttactgtga
      781 cagctgaagc tttacgtttt cggcaaatac agaggggatt tcgtacaaca ctggatgatc
      841 tcagtgggcg ttcttatgta atgactgctg aagatgttga tcttacattg aactggggaa
      901 ggttgagtag cgtcctgcct gactatcatg gacaagactc tgttcgtgta ggaagaattt
      961 cttttggaag cattaatgca attctgggaa gcgtggcatt aatactgaat tgtcatcatc
     1021 atgcatcgcg agttgccaga atggcatctg atgagtttcc ttctatgtgt ccggcagatg
     1081 gaagagtccg tgggattacg cacaataaaa tattgtggga ttcatccact ctgggggcaa
     1141 ttctgatgcg cagaactatt agcagttgag ggggtaaaat gaaaaaaaca ttattaatag
     1201 ctgcatcgct ttcatttttt tcagcaagtg cgctggcgac gcctgattgt gtaactggaa
     1261 aggtggagta tacaaaatat aa
//
LOCUS       DAZKHF020000534         1275 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2201S31241
            SAMN43267800-rid25106413.denovo.538, whole genome shotgun sequence.
ACCESSION   DAZKHF020000534 DAZKHF020000000
VERSION     DAZKHF020000534.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267800
            Sequence Read Archive: SRR30307263
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1275)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1275)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 1275)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350581.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350581.2
            Genome Coverage       :: 151x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:21:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,652
            CDSs (total)                      :: 5,557
            Genes (coding)                    :: 5,320
            CDSs (with protein)               :: 5,320
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 237
            CDSs (without protein)            :: 237
            Pseudo Genes (ambiguous residues) :: 0 of 237
            Pseudo Genes (frameshifted)       :: 102 of 237
            Pseudo Genes (incomplete)         :: 130 of 237
            Pseudo Genes (internal stop)      :: 52 of 237
            Pseudo Genes (multiple problems)  :: 41 of 237
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1275
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267800-rid25106413.denovo.538"
                     /serotype="O 26:H11"
                     /isolate="2201S31241"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-01"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            197..481
                     /locus_tag="ACHOSC_RS25600"
                     /old_locus_tag="pgaptmp_005120"
                     /pseudo
     CDS             197..481
                     /locus_tag="ACHOSC_RS25600"
                     /old_locus_tag="pgaptmp_005120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_459368.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            548..1275
                     /locus_tag="ACHOSC_RS25605"
                     /old_locus_tag="pgaptmp_005121"
                     /pseudo
     CDS             548..1275
                     /locus_tag="ACHOSC_RS25605"
                     /old_locus_tag="pgaptmp_005121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477168.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3-like element IS3 family transposase"
BASE COUNT          355 a          316 c          304 g          300 t
ORIGIN      
        1 tgaaatatcg cccttttatc aatttgatgc gtgtttttct ccataggtta tatatgtcca
       61 cattcggact taggggaaag aataattgaa ccattcgtct gtaacgcagc ataatcatta
      121 gcgcgaaaca taatatgttt tctatgcatt gataattgat ggatcaactt attacgtccc
      181 tgaggaggga tgacaaatgc actcctggaa aaagaaactt gtagtatcac aattagcatt
      241 ggcttgcact ctggctatca cctctcaggc taatgcaact acttatgaca cctggactta
      301 ttacgacaat ccaactaccg ctcttgactg gagcaatatg gatgcaccgg gtactgtaga
      361 tggtaactat gtgaattata gtggctttgt ctactataac aacacaaacg gtgatttcga
      421 taccaccttt aatggtgaca ctgttaacgg gactatctct acatattacc tgaatcatga
      481 cttgatctta cccagcaata gtggacacgc ggctaagtga gtaaactctc agtcagaggt
      541 gactcacatg acaaaaacag tatcaaccag taaaaaaccc cgtaaacagc attcgcctga
      601 atttcgcagt gaagccctga agcttgctga acgcatcggt gttactgccg cagcccgtga
      661 actcagcctg tatgaatcac agctctacaa ctggcgcagt aaacagcaaa atcagcagac
      721 gtcttctgaa cgtgaactgg agatgtctac cgagattgca cgtctcaaac gccagctggc
      781 agaacgggat gaagagctgg ctatcctcca aaaggccgcg acatacttcg cgaagcgcct
      841 gaaatgaagt atgtctttat tgaaaaacat caggctgagt tcagcatcaa agcaatgtgc
      901 cgcgtgctcc gggtggcccg cagcggctgg tatacgtggt gtcagcggcg gacaaggata
      961 agcacgcgtc agcagttccg ccaacactgc gacagcgttg tcctcgcggc ttttacccgg
     1021 tcaaaacagc gttacggtgc cccacgcctg acggatgaac tgcgtgctca gggttacccc
     1081 tttaacgtaa aaaccgtggc ggcaagcctg cgccgtcagg gactgagggc aaaggcctcc
     1141 cggaagttca gcccggtcag ctaccgcgca cacggcctgc ctgtgtcaga aaatctgttg
     1201 gagcaggatt tttacgccag tggcccgaac cagaagtggg caggagacat cacgtactta
     1261 cgtacagatg aaggc
//
LOCUS       DAZKHF020000535         1272 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2201S31241
            SAMN43267800-rid25106413.denovo.539, whole genome shotgun sequence.
ACCESSION   DAZKHF020000535 DAZKHF020000000
VERSION     DAZKHF020000535.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267800
            Sequence Read Archive: SRR30307263
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1272)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1272)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 1272)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350581.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350581.2
            Genome Coverage       :: 151x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:21:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,652
            CDSs (total)                      :: 5,557
            Genes (coding)                    :: 5,320
            CDSs (with protein)               :: 5,320
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 237
            CDSs (without protein)            :: 237
            Pseudo Genes (ambiguous residues) :: 0 of 237
            Pseudo Genes (frameshifted)       :: 102 of 237
            Pseudo Genes (incomplete)         :: 130 of 237
            Pseudo Genes (internal stop)      :: 52 of 237
            Pseudo Genes (multiple problems)  :: 41 of 237
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1272
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267800-rid25106413.denovo.539"
                     /serotype="O 26:H11"
                     /isolate="2201S31241"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-01"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            10..>1272
                     /gene="espK"
                     /locus_tag="ACHOSC_RS25610"
                     /old_locus_tag="pgaptmp_005122"
     CDS             10..>1272
                     /gene="espK"
                     /locus_tag="ACHOSC_RS25610"
                     /old_locus_tag="pgaptmp_005122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system effector EspK"
                     /protein_id="HGU4550861.1"
                     /translation="MLPTSQLRPSGTFYSNSAETSAGIKSEITPTQIEEARAIGRLYI
                     KDCDIEYLPQLPNEITSVTIENCNNLTTLTGLPVNTQNLSVINCEKLQITDIPSTVKN
                     LHIELTDSPFIHIISEGIECLTVCHCHISGVPESVRHLEIRGYATDGIKNVPNGLLSL
                     SINRYTPENQARIDSLISPSLQTLSLTGCSNIILPEKLPESLRSVTIHVEQKTTWNIV
                     AEGVPDGLDLDLQNVLLSPNVIKEKNITFQGNALDAALHFRPGDIVYGLSSPREKLVN
                     SIKLVNDFSKKDIVIQNTLTNAVWDARTPRKYKQDSLIKRALNEHERGTKFKQCLKNH
                     NNYNVIMADLSIYNRDKLWAKTSKAGLEFQTLIRNRTVMFCADGLVNSLKLIANKSEG
                     YGQSITASELRWIYRNKDNSQIMKNIKFY"
BASE COUNT          448 a          281 c          232 g          311 t
ORIGIN      
        1 aatttatcaa tgcttcctac atcgcaatta cgaccgtccg ggacattcta ctccaattcc
       61 gcagaaacat cagcaggcat caaaagcgaa atcacaccaa ctcagataga agaagcgcgg
      121 gccattggtc gtttatatat caaagattgt gatattgagt atctgccaca gttaccaaac
      181 gaaataacat cagttacaat cgaaaattgc aataacctga ccacccttac aggattaccg
      241 gttaatacac aaaacctgtc cgtcattaac tgtgaaaaat tacaaatcac agacatacca
      301 tcgacagtaa aaaatctaca tattgaatta actgattcac catttataca catcatatct
      361 gaaggcatcg aatgcctgac ggtttgccac tgccatatat ctggagtgcc agagagtgtc
      421 cgtcatcttg agatcagagg ttacgccaca gacggcataa aaaatgttcc aaacgggtta
      481 ttatctctca gcatcaatcg ctacacccca gaaaatcagg ccagaattga cagcctgata
      541 tcaccgtcac tacagacgct atctctgact ggatgtagca atattatact gccggagaaa
      601 ctcccggaga gtctgagatc tgtaaccatt catgtggagc agaaaactac atggaacatc
      661 gtagctgaag gggttcctga tggactggat cttgatttac aaaatgtact actctctccg
      721 aatgtaatta aagaaaaaaa catcactttt cagggcaacg cactggatgc tgccttgcat
      781 tttcgtccgg gagacattgt ctatgggcta tcctctccca gagaaaaact tgtcaacagc
      841 atcaaactgg ttaacgactt ttccaaaaaa gatatcgtaa ttcagaatac attaacaaac
      901 gcagtatggg acgccagaac cccccgtaaa tataagcaag actcgcttat caaaagagca
      961 ttaaatgaac atgaaagagg aacaaaattt aaacagtgct taaaaaatca caataattat
     1021 aatgtcatca tggccgacct ttccatatac aatcgagaca agttatgggc aaaaacaagc
     1081 aaggcaggcc tagagtttca aacattaatt cgcaacagaa cggttatgtt ttgtgcggat
     1141 gggcttgtca attcactcaa actcatagct aacaagtcag agggctatgg tcagagtatt
     1201 accgccagcg aattacgatg gatttatcgt aataaagaca acagccaaat aatgaaaaac
     1261 ataaaatttt at
//
LOCUS       DAZKHF020000536         1272 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2201S31241
            SAMN43267800-rid25106413.denovo.540, whole genome shotgun sequence.
ACCESSION   DAZKHF020000536 DAZKHF020000000
VERSION     DAZKHF020000536.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267800
            Sequence Read Archive: SRR30307263
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1272)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1272)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 1272)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350581.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350581.2
            Genome Coverage       :: 151x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:21:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,652
            CDSs (total)                      :: 5,557
            Genes (coding)                    :: 5,320
            CDSs (with protein)               :: 5,320
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 237
            CDSs (without protein)            :: 237
            Pseudo Genes (ambiguous residues) :: 0 of 237
            Pseudo Genes (frameshifted)       :: 102 of 237
            Pseudo Genes (incomplete)         :: 130 of 237
            Pseudo Genes (internal stop)      :: 52 of 237
            Pseudo Genes (multiple problems)  :: 41 of 237
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1272
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267800-rid25106413.denovo.540"
                     /serotype="O 26:H11"
                     /isolate="2201S31241"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-01"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            38..403
                     /locus_tag="ACHOSC_RS25615"
                     /old_locus_tag="pgaptmp_005123"
     CDS             38..403
                     /locus_tag="ACHOSC_RS25615"
                     /old_locus_tag="pgaptmp_005123"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4550862.1"
                     /translation="MSRSAKPRKRKPAPQRSKLPRYVVKLHDDDFFDEEDAEALRFDN
                     FDDAVECCADLNIPFFVDAGNKKLVFWFVRVDDEGYPEIARCTEREFATILAGISAGG
                     MYCPECGTVHWPDGVPPPF"
     gene            403..>1272
                     /locus_tag="ACHOSC_RS25620"
                     /old_locus_tag="pgaptmp_005124"
     CDS             403..>1272
                     /locus_tag="ACHOSC_RS25620"
                     /old_locus_tag="pgaptmp_005124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013747467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS91 family transposase"
                     /protein_id="HGU4550863.1"
                     /translation="MLPRFADIFQQGNRWLNWLEKQPEGSVRPVVIESVTKIMACGTT
                     LMGYTQWCCSSPDCSHIKKVCFRCKSRSCPHCGVKAGAQWIQYLLSLVPDCPWQHIVF
                     TLPCQYWSLVFHNRRLLAEMSRIAADVIQEICRQADVVPGIFTVIHTWGRDQEWHPHI
                     HLSTTTGGVTSDHTWKNLHFYARKVMSMWRYRITRLLSRKYPDLVIPDALAAEGSSKR
                     DWNRFLDSHYRRGWNVNVSRVMDNATHVAVYFGSYLKKPPVPMSRLEHYAGQDEIGLR
                     YNSHRTKREEYLVM"
BASE COUNT          298 a          325 c          360 g          289 t
ORIGIN      
        1 taaaaacaga caatatcatt gtctttcagg tagttatatg tcccgttcag ctaaaccccg
       61 taaacgaaaa cctgcccctc aaagaagcaa acttccccgc tatgtcgtga agcttcacga
      121 cgatgacttc tttgacgaag aagacgcaga agctctgcgc tttgataatt ttgacgatgc
      181 cgttgagtgc tgcgcagacc tgaatattcc cttctttgtg gatgccggaa acaaaaagct
      241 ggtcttctgg tttgtacgtg ttgatgacga agggtatcct gaaatagccc gctgcacgga
      301 gcgggagttt gcgaccattc ttgccggtat cagcgccggc ggcatgtact gcccggagtg
      361 tggcacggtt cactggccgg acggagtccc cccgcccttc tgatgcttcc ccgttttgcc
      421 gacatttttc agcagggaaa ccgctggctt aactggctgg agaaacaacc ggaaggttca
      481 gtgcgtccgg tagtcattga gtctgtgaca aaaatcatgg cctgcgggac cacgctgatg
      541 gggtacacac agtggtgctg ttcatctccg gactgcagcc acataaaaaa ggtctgcttc
      601 cggtgtaaaa gtcgctcctg cccgcactgc ggagtgaagg ctggcgcaca gtggatacag
      661 tatctgctga gtctggttcc cgactgtccg tggcagcata ttgtgttcac acttccctgc
      721 cagtactggt ccctggtgtt ccacaaccgg aggttactgg cagagatgag ccgcattgct
      781 gcggatgtga tacaggaaat ctgccgccag gcagatgtgg tgccggggat attcacggtg
      841 atccacacat ggggacgtga ccaggagtgg catccgcaca ttcacctgtc gacaacgacc
      901 ggcggcgtga catcagacca cacctggaaa aaccttcatt tttacgcccg taaggtgatg
      961 agtatgtggc gttaccggat aacgcggtta ctgtcacgga aatatccgga cctggtgata
     1021 ccggatgcgc tggcagcaga aggaagcagt aaacgggact ggaatcgctt cctggacagt
     1081 cattaccggc ggggctggaa tgtcaacgta tcccgggtga tggataacgc cacacatgtg
     1141 gcggtgtact tcggctctta cctgaaaaaa ccgccggtgc cgatgagccg tctggagcac
     1201 tatgctggtc aggatgaaat tggtctgcgt tacaacagtc accggacaaa acgggaagaa
     1261 tacctggtga tg
//
LOCUS       DAZKHF020000537         1270 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2201S31241
            SAMN43267800-rid25106413.denovo.541, whole genome shotgun sequence.
ACCESSION   DAZKHF020000537 DAZKHF020000000
VERSION     DAZKHF020000537.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267800
            Sequence Read Archive: SRR30307263
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1270)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1270)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 1270)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350581.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350581.2
            Genome Coverage       :: 151x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:21:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,652
            CDSs (total)                      :: 5,557
            Genes (coding)                    :: 5,320
            CDSs (with protein)               :: 5,320
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 237
            CDSs (without protein)            :: 237
            Pseudo Genes (ambiguous residues) :: 0 of 237
            Pseudo Genes (frameshifted)       :: 102 of 237
            Pseudo Genes (incomplete)         :: 130 of 237
            Pseudo Genes (internal stop)      :: 52 of 237
            Pseudo Genes (multiple problems)  :: 41 of 237
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1270
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267800-rid25106413.denovo.541"
                     /serotype="O 26:H11"
                     /isolate="2201S31241"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-01"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            <1..633
                     /locus_tag="ACHOSC_RS25625"
                     /old_locus_tag="pgaptmp_005125"
     CDS             <1..633
                     /locus_tag="ACHOSC_RS25625"
                     /old_locus_tag="pgaptmp_005125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="terminase gpA endonuclease subunit"
                     /protein_id="HGU4550864.1"
                     /translation="HDDEQTLLRVDEAINKTYTRRNGAEMSVSRICWDTGGIDPTIVY
                     ERSKKHGLFRVIPIKGASVYGKPVASMPRKRNKNGVYLTEIGTDTAKEQIYNRFTLTP
                     EGDEPLPGAVHFPNNPDIFDLTEAQQLTAEEQVEKWVDGRKKILWDSKKRRNEALDCF
                     VYALAALRISISRWQLDLSALLASLQEEDGAATNKKTLAEYARALSGEDE"
     gene            630..836
                     /locus_tag="ACHOSC_RS25630"
                     /old_locus_tag="pgaptmp_005126"
     CDS             630..836
                     /locus_tag="ACHOSC_RS25630"
                     /old_locus_tag="pgaptmp_005126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0019058 - viral life cycle [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage head-tail joining protein"
                     /protein_id="HGU4550865.1"
                     /translation="MTRQEELAAARAALHDLMTGKRVATVQKDGRRVEFTTTSVSDLK
                     KYIAELEVQTGMTQRRRGPAGFYV"
     gene            833..>1270
                     /locus_tag="ACHOSC_RS25635"
                     /old_locus_tag="pgaptmp_005127"
     CDS             833..>1270
                     /locus_tag="ACHOSC_RS25635"
                     /old_locus_tag="pgaptmp_005127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HGU4550866.1"
                     /translation="MKMSTIPTLLGPDGMTSLREYAGYHGGGSGFGGQLRAWNPPGES
                     VDAALLPNFTRGNARADDLVRNNGYAANAIQLHQDHIVGSFFRLSHRPSWRYLGIGEE
                     EARAFSREVEAAWKEFAEDDCCCIDVERKRTFTMMIREGVAMHA"
BASE COUNT          319 a          323 c          379 g          249 t
ORIGIN      
        1 cacgacgatg aacagacgct gctgcgtgtg gatgaggcca tcaataaaac ctatacccgc
       61 cggaatggtg cagaaatgtc ggtatcccgt atctgctggg atactggcgg gattgacccg
      121 accattgtgt atgaacgctc gaaaaaacat gggctgttcc gggtgatccc cattaaaggg
      181 gcatccgtct acggaaagcc ggtggccagc atgccacgta agcgaaacaa aaacggggtt
      241 taccttaccg aaatcggtac ggataccgcg aaagagcaga tttataaccg cttcacactg
      301 acgccggaag gggatgaacc gcttcccggt gccgttcact tcccgaataa cccggatatt
      361 tttgatctta ccgaagcgca gcaactgact gctgaagagc aggtcgaaaa atgggtggat
      421 ggcaggaaaa aaatactgtg ggacagcaaa aagcgacgca atgaggcgct cgactgcttc
      481 gtttatgcgc tggcggcgct gcgcatcagt atttcccgct ggcagctgga tctcagtgca
      541 ctgctggcga gcctgcagga agaggatggt gcagcaacca acaagaaaac actggcagaa
      601 tacgcccgtg ccttatccgg agaggatgaa tgacgcgaca ggaagaactt gccgctgccc
      661 gtgcggcact gcatgacctg atgacaggaa aacgggtggc aacggtacag aaagacggac
      721 ggcgagtgga gtttacgacc acttccgtgt ctgacctgaa aaaatacatt gctgagctgg
      781 aagtgcagac cggcatgaca cagcgacgca ggggaccagc aggattttat gtatgaaaat
      841 gtccaccatt cccacccttc tggggccgga cggcatgaca tcgctgcgtg aatatgccgg
      901 ttatcacggc ggtggcagcg gatttggtgg gcagttgcgg gcgtggaacc caccgggtga
      961 aagtgtggat gcagccctgc tgcccaactt tacccgtggc aatgcccgcg cagacgatct
     1021 ggtacgcaat aacggctatg ccgccaacgc catccagttg catcaggatc atatcgtcgg
     1081 gtcttttttc cgactcagtc atcgcccaag ctggcgctat ctgggcatcg gggaggaaga
     1141 agcccgtgcc ttttcccgcg aggttgaagc ggcatggaaa gagtttgccg aagatgactg
     1201 ttgctgcatt gacgttgagc gaaaacgcac gtttaccatg atgattcggg aaggtgtggc
     1261 catgcacgcc
//
LOCUS       DAZKHF020000538         1265 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2201S31241
            SAMN43267800-rid25106413.denovo.542, whole genome shotgun sequence.
ACCESSION   DAZKHF020000538 DAZKHF020000000
VERSION     DAZKHF020000538.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267800
            Sequence Read Archive: SRR30307263
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1265)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1265)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 1265)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350581.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350581.2
            Genome Coverage       :: 151x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:21:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,652
            CDSs (total)                      :: 5,557
            Genes (coding)                    :: 5,320
            CDSs (with protein)               :: 5,320
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 237
            CDSs (without protein)            :: 237
            Pseudo Genes (ambiguous residues) :: 0 of 237
            Pseudo Genes (frameshifted)       :: 102 of 237
            Pseudo Genes (incomplete)         :: 130 of 237
            Pseudo Genes (internal stop)      :: 52 of 237
            Pseudo Genes (multiple problems)  :: 41 of 237
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1265
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267800-rid25106413.denovo.542"
                     /serotype="O 26:H11"
                     /isolate="2201S31241"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-01"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            complement(<1..643)
                     /locus_tag="ACHOSC_RS25640"
                     /old_locus_tag="pgaptmp_005128"
     CDS             complement(<1..643)
                     /locus_tag="ACHOSC_RS25640"
                     /old_locus_tag="pgaptmp_005128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030430 - host cell cytoplasm [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0046718 - symbiont entry into host cell
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage minor tail protein L"
                     /protein_id="HGU4550867.1"
                     /translation="MQDIRQETLNECTRAEQSASVVLWEIDLTEVGGERYFFCNEQNE
                     KGEPVTWQGRQYQAYPIQGTGFELNGKGSAARPTLTVSNLHGMVTGMVEDMQSLVGGT
                     VVRRKVYARFLDAVNFVNGNSYADPEQEVISRWRIEQCSELSAVSASFVLSTPTETDG
                     AVFPGRIMLANTCTWTYRGDECGYSGPAVADEYDQPTSDITKDKCSKCLSGCKF"
     gene            complement(643..972)
                     /locus_tag="ACHOSC_RS25645"
                     /old_locus_tag="pgaptmp_005129"
     CDS             complement(643..972)
                     /locus_tag="ACHOSC_RS25645"
                     /old_locus_tag="pgaptmp_005129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HGU4550868.1"
                     /translation="MKTFRWKVKPGMDVASAPSVRKVRFGDGYSQRAPAGLNANLKTY
                     SVTLSVPREEATVLESFLEEHGGWKSFLWTPPYEWRQIKVTCAKWSSRVSMLRVEFSA
                     EFEQVVN"
     gene            complement(969..>1265)
                     /locus_tag="ACHOSC_RS25650"
                     /old_locus_tag="pgaptmp_005130"
     CDS             complement(969..>1265)
                     /locus_tag="ACHOSC_RS25650"
                     /old_locus_tag="pgaptmp_005130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tape measure protein"
                     /protein_id="HGU4550869.1"
                     /translation="FVFTKEATSRIGVGNLYRLMRGYATGGYVGTPGSLADSRSQASG
                     TFEQNNHVVINNDGTNGQIGPAALKAVYDMARKGARDEIQTQMRDGGLFSGGGR"
BASE COUNT          266 a          420 c          303 g          276 t
ORIGIN      
        1 ggaacttaca accgctcagg catttgctgc atttatcctt cgtgatatcg gacgttggct
       61 ggtcatattc atccgcgaca gccggaccgc tataaccgca ctcgtcaccg cgataggtcc
      121 aggtgcaggt gttggccagc atgatacgtc ccggaaaaac agcgccatcc gtttccgtcg
      181 gcgtggacag tacaaaggag gcactcaccg cgctcagttc gctgcactgc tcaatgcgcc
      241 agcggctgat cacctcctgc tccggatcgg cgtaactgtt tccgttgacg aagttcaccg
      301 catccagaaa acgggcgtaa accttacgcc tgaccaccgt tccgccgacc agactctgca
      361 tatcttccac cataccggtg accatgccgt gcaggttaga aaccgtcagt gtcggacggg
      421 cagcactgcc cttgccgttc agttcaaatc ccgtcccctg aatgggatac gcctgatact
      481 gtcgcccctg ccaggtgacc ggctcacctt tttcgttctg ctcattacag aaaaaataac
      541 gttctccacc gacctctgtc aggtcgattt cccagagcac cacgctggcc gactgctccg
      601 cacgggtgca ttcattcagt gtttcctgcc ggatatcctg catcagttca ccacctgttc
      661 aaactctgcg ctgaactcaa cacgcagcat actgacccgc gacgaccatt ttgcgcaggt
      721 cacctttatc tgccgccact cataaggcgg cgtccacaga aaggatttcc agcccccgtg
      781 ctcttccaga aacgactcca gtaccgtggc ctcctcacgg gggacagaaa gcgtcacgct
      841 gtacgttttc aggttggcat tcagcccggc aggcgctcgc tgggaatagc catcaccaaa
      901 gcgcaccttt cttacagaag gggccgaagc cacatccata ccgggtttca ctttccagcg
      961 gaaggttttc atcgtccacc tccggagaac aggccaccat cacgcatctg tgtctgaatt
     1021 tcatcacggg cacccttgcg ggccatgtca tacaccgcct tcagagcagc cggacctatc
     1081 tgcccgttcg tgccgtcgtt gttaatcacc acatggttat tctgctcaaa cgtcccggac
     1141 gcctgcgacc ggctgtcagc cagactgccc ggtgtaccga cataaccacc ggtggcatag
     1201 ccgcgcatca gccggtagag attccccacg ccaatccggc tggttgcctc cttcgtgaag
     1261 acaaa
//
LOCUS       DAZKHF020000539         1265 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2201S31241
            SAMN43267800-rid25106413.denovo.543, whole genome shotgun sequence.
ACCESSION   DAZKHF020000539 DAZKHF020000000
VERSION     DAZKHF020000539.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267800
            Sequence Read Archive: SRR30307263
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1265)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1265)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 1265)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350581.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350581.2
            Genome Coverage       :: 151x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:21:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,652
            CDSs (total)                      :: 5,557
            Genes (coding)                    :: 5,320
            CDSs (with protein)               :: 5,320
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 237
            CDSs (without protein)            :: 237
            Pseudo Genes (ambiguous residues) :: 0 of 237
            Pseudo Genes (frameshifted)       :: 102 of 237
            Pseudo Genes (incomplete)         :: 130 of 237
            Pseudo Genes (internal stop)      :: 52 of 237
            Pseudo Genes (multiple problems)  :: 41 of 237
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1265
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267800-rid25106413.denovo.543"
                     /serotype="O 26:H11"
                     /isolate="2201S31241"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-01"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            complement(<1..643)
                     /locus_tag="ACHOSC_RS25655"
                     /old_locus_tag="pgaptmp_005131"
     CDS             complement(<1..643)
                     /locus_tag="ACHOSC_RS25655"
                     /old_locus_tag="pgaptmp_005131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030430 - host cell cytoplasm [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0046718 - symbiont entry into host cell
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage minor tail protein L"
                     /protein_id="HGU4550870.1"
                     /translation="MQDIRQETLNECTRAEQSASVVLWEIDLTEVGGERYFFCNEQNE
                     KGEPVTWQGRQYQPYPIQGSGFELNGKGTSTRPTLTVSNLYGMVTGMAEDLQSLVGGT
                     VVRRKVYARFLDAVNFVNGNSDADPEQEVISRWRIEQCSELSAVSASFVLSTPTETDG
                     AVFPGRIMLANTCTWTYRGDECGYSGPAVADEYDQPTSDITKDKCSKCLSGCKF"
     gene            complement(643..972)
                     /locus_tag="ACHOSC_RS25660"
                     /old_locus_tag="pgaptmp_005132"
     CDS             complement(643..972)
                     /locus_tag="ACHOSC_RS25660"
                     /old_locus_tag="pgaptmp_005132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HGU4550871.1"
                     /translation="MKTFRWKVKPGMDVTSAPSVREVRFGDGYSQRVPAGLNADLKTY
                     SVTLSVSREEATALESFLAEHGGWKAFLWTPPYGYRQIKVTCAKWSSQVSMLRVEFSA
                     EFKQVVN"
     gene            complement(969..>1265)
                     /locus_tag="ACHOSC_RS25665"
                     /old_locus_tag="pgaptmp_005133"
     CDS             complement(969..>1265)
                     /locus_tag="ACHOSC_RS25665"
                     /old_locus_tag="pgaptmp_005133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tape measure protein"
                     /protein_id="HGU4550872.1"
                     /translation="FVFTKEATSRIGVGNLYRLMRGYATGGYVGTPGSMADSRSQASG
                     TFEQNNHVVINNDGTNGQIGPAALKAVYDMARKGARDEIQTQMRDGGLFSGGGR"
BASE COUNT          265 a          421 c          307 g          272 t
ORIGIN      
        1 ggaacttaca accgctcagg catttgctgc atttatcctt cgtgatatcg gacgttggct
       61 ggtcatattc atccgcgaca gccggaccgc tataaccgca ctcgtcaccg cgataggtcc
      121 aggtgcaggt gttggccagc atgatacgtc ccggaaaaac agcgccatcc gtttccgtcg
      181 gcgtggacag tacaaaggag gcactcaccg cgctcagttc gctgcactgc tcgatgcgcc
      241 agcggctgat cacctcctgc tccggatcgg cgtcactgtt tccgttgacg aagttcaccg
      301 catccagaaa acgggcgtaa accttacgcc ggaccaccgt tccgccgacc agactctgca
      361 gatcttccgc catcccggtg accataccgt acaggttaga aaccgtcagc gtggggcgcg
      421 tactggtgcc tttgccattc agttcaaaac cgctcccctg aatgggatac ggctgatact
      481 gtcgcccctg ccaggtgacc ggctcacctt tttcgttctg ctcattacag aaaaaataac
      541 gttcaccacc gacctctgtc agatcgattt cccagagcac cacgctggcc gactgctccg
      601 cacgggtgca ttcattcagt gtttcctgcc ggatatcctg catcagttca ccacctgttt
      661 aaactctgcg ctgaactcaa cacgcaacat actgacctgc gacgaccatt ttgcgcaggt
      721 cacctttatc tgcctgtaac cataaggcgg cgtccacaga aaggccttcc agcccccgtg
      781 ctcagccaga aacgactcca gcgccgtggc ctcctcacgg gagacagaca gcgtcacgct
      841 gtacgttttc aggtcagcgt tcagcccggc aggcacacgc tgggaatagc catcaccaaa
      901 gcgcacctcc ctgacggaag gggccgatgt cacatccata ccgggtttca ctttccagcg
      961 gaaggttttc atcgtccacc tccggagaac aggccaccat cacgcatctg tgtctgaatt
     1021 tcatcacggg cacccttgcg ggccatgtca tacaccgcct tcagagcagc cggacctatc
     1081 tgcccgttcg tgccgtcgtt gttaatcacc acatggttat tctgctcaaa cgtcccggac
     1141 gcctgcgacc ggctgtctgc catgctgccc ggtgtaccga cataaccgcc ggtggcatag
     1201 ccgcgcatca gccggtaaag attccccacg ccaatccggc tggttgcctc cttcgtgaag
     1261 acaaa
//
LOCUS       DAZKHF020000540         1239 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2201S31241
            SAMN43267800-rid25106413.denovo.544, whole genome shotgun sequence.
ACCESSION   DAZKHF020000540 DAZKHF020000000
VERSION     DAZKHF020000540.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267800
            Sequence Read Archive: SRR30307263
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1239)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1239)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 1239)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350581.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350581.2
            Genome Coverage       :: 151x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:21:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,652
            CDSs (total)                      :: 5,557
            Genes (coding)                    :: 5,320
            CDSs (with protein)               :: 5,320
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 237
            CDSs (without protein)            :: 237
            Pseudo Genes (ambiguous residues) :: 0 of 237
            Pseudo Genes (frameshifted)       :: 102 of 237
            Pseudo Genes (incomplete)         :: 130 of 237
            Pseudo Genes (internal stop)      :: 52 of 237
            Pseudo Genes (multiple problems)  :: 41 of 237
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1239
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267800-rid25106413.denovo.544"
                     /serotype="O 26:H11"
                     /isolate="2201S31241"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-01"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            <1..494
                     /locus_tag="ACHOSC_RS25670"
                     /old_locus_tag="pgaptmp_005134"
     CDS             <1..494
                     /locus_tag="ACHOSC_RS25670"
                     /old_locus_tag="pgaptmp_005134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="terminase small subunit"
                     /protein_id="HGU4550873.1"
                     /translation="AAGYSAKTARVKGSQLAKDPEVLAFIARKQCETVEVDEVPVYRQ
                     KKSEPEDKPRRREAAAIPQPDETNPEMPPPVVISPGIEYMEDGLPDPVKAMGRLLVEN
                     INTDPRLALDAAYKLAQFTHHKKGDAGKKSAKGDAAKKAANRFAVPPPPRLVVNNDNE
                     GNG"
     gene            491..>1239
                     /locus_tag="ACHOSC_RS25675"
                     /old_locus_tag="pgaptmp_005135"
     CDS             491..>1239
                     /locus_tag="ACHOSC_RS25675"
                     /old_locus_tag="pgaptmp_005135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase large subunit"
                     /protein_id="HGU4550874.1"
                     /translation="MIPVWSTACPDWAERLKKGLSIIPAPIYPEQAAHALAIFKQLRI
                     VDAPGSPTFGESCAQWVFDLVAALFGSYDAQTGVRHIKEVFILIPKKNSKSTLAAGIM
                     MTALLLNWRQAAGYTILAPTVEVAANAFNPARDMVRRDDDLDDLCQVQTHIRTITHRV
                     TDTTLKVVAADPNTVSGIKSVGTLIDELWLFGKQYKAEDMLREAIGGLASRPEGFVVY
                     TTTQSNEPPAGVFRQKLQYARDVRDGKIHDP"
BASE COUNT          289 a          323 c          387 g          240 t
ORIGIN      
        1 ttgccgccgg ttattcggcg aagacagcca gggttaaagg ctcgcagctg gcaaaagatc
       61 cggaggtgct tgcgtttata gcccgtaaac agtgcgagac ggtggaggtg gatgaggttc
      121 ctgtttaccg gcagaaaaaa tcagagccgg aggataaacc ccgtcgccgt gaggcggctg
      181 caataccaca gccggacgaa acaaatccgg agatgccacc gcccgtggtg atatctcctg
      241 gtattgagta tatggaggac ggtcttcccg atccggtgaa agcgatgggg cgtcttctgg
      301 tggagaacat taataccgac cccaggctgg cgctggatgc ggcttataag ctggcgcaat
      361 tcacgcacca caaaaaaggg gatgccggta aaaaatcggc aaaaggtgac gcggcgaaaa
      421 aagcggctaa ccgttttgcg gtgccaccac caccccgcct ggtggtgaat aatgataatg
      481 agggcaacgg atgatacctg tgtggagcac ggcctgcccg gactgggcag agcgcctgaa
      541 aaaggggctg tcgattattc cggctccgat ttatccggag caggccgcac atgccctggc
      601 gatttttaaa caactgcgga ttgtggatgc accgggcagc ccgacgttcg gggagtcctg
      661 cgcacagtgg gtgtttgacc tggtggcggc cctgtttggc tcctacgatg cgcagaccgg
      721 tgtacgccat atcaaggaag tttttatcct tatccccaag aaaaacagca agtccacgct
      781 ggctgccggg atcatgatga cggcgctgtt actgaactgg cggcaggcgg cgggctacac
      841 cattctggcc ccgaccgtgg aggtggcggc taacgccttc aaccctgcca gggatatggt
      901 acgacgggac gatgatctgg atgacctctg tcaggtgcag acacatatcc ggaccatcac
      961 ccacagggtg acggacacca cccttaaggt ggtggctgcc gatccgaata cggtatccgg
     1021 tatcaagtcc gtggggacgc tgattgatga actgtggtta tttggcaagc agtacaaagc
     1081 ggaggacatg ctacgtgaag ccataggcgg ccttgcctcc cgcccggaag ggtttgtggt
     1141 gtatacgacc acccagtcga atgagccgcc agccggggtg ttcagacaga aactgcagta
     1201 cgcccgggat gtccgtgacg gcaaaattca tgatccgca
//
LOCUS       DAZKHF020000541         1235 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2201S31241
            SAMN43267800-rid25106413.denovo.545, whole genome shotgun sequence.
ACCESSION   DAZKHF020000541 DAZKHF020000000
VERSION     DAZKHF020000541.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267800
            Sequence Read Archive: SRR30307263
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1235)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1235)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 1235)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350581.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350581.2
            Genome Coverage       :: 151x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:21:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,652
            CDSs (total)                      :: 5,557
            Genes (coding)                    :: 5,320
            CDSs (with protein)               :: 5,320
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 237
            CDSs (without protein)            :: 237
            Pseudo Genes (ambiguous residues) :: 0 of 237
            Pseudo Genes (frameshifted)       :: 102 of 237
            Pseudo Genes (incomplete)         :: 130 of 237
            Pseudo Genes (internal stop)      :: 52 of 237
            Pseudo Genes (multiple problems)  :: 41 of 237
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1235
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267800-rid25106413.denovo.545"
                     /serotype="O 26:H11"
                     /isolate="2201S31241"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-01"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            41..763
                     /gene="sdiA"
                     /locus_tag="ACHOSC_RS25680"
                     /old_locus_tag="pgaptmp_005136"
     CDS             41..763
                     /gene="sdiA"
                     /locus_tag="ACHOSC_RS25680"
                     /old_locus_tag="pgaptmp_005136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator SdiA"
                     /protein_id="HGU4550875.1"
                     /translation="MQDTDFFSWRRTMLLRFQRMEAAEEVYHEIELQAQQLEYDYYSL
                     CVRHPVPFTRPKVAFYTNYPEAWVSYYQAKNFLAIDPVLNPENFSQGHLMWNDDLFNE
                     AQPLWEAARAHGLRRGVTQYLMLPNRALGFLSFSRCSAREIPILSDELQLKMQLLVRE
                     SLMALMRLNDEIVMTPEMNFSKREKEILRWTAEGKTSAEIAMILSISENTVNFHQKNM
                     QKKINAPNKTQVACYAAATGLI"
     gene            complement(831..1055)
                     /gene="yecF"
                     /locus_tag="ACHOSC_RS25685"
                     /old_locus_tag="pgaptmp_005137"
     CDS             complement(831..1055)
                     /gene="yecF"
                     /locus_tag="ACHOSC_RS25685"
                     /old_locus_tag="pgaptmp_005137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2594 family protein YecF"
                     /protein_id="HGU4550876.1"
                     /translation="MSTPDFSTAENNQELANEVSCLKAMLTLMLQAMGQADAGRVMLK
                     MEKQLALIEDETQAAVFSKTVKQIKQAYRQ"
     gene            complement(1042..1218)
                     /gene="yecU"
                     /locus_tag="ACHOSC_RS25690"
                     /old_locus_tag="pgaptmp_005138"
     CDS             complement(1042..1218)
                     /gene="yecU"
                     /locus_tag="ACHOSC_RS25690"
                     /old_locus_tag="pgaptmp_005138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YecU"
                     /protein_id="HGU4550877.1"
                     /translation="MIKIFIGHYINAFNSTVDDTLKKQPLLFLAKLMVYSAALTLFTA
                     NFHCNMTRKINEYA"
BASE COUNT          320 a          267 c          296 g          352 t
ORIGIN      
        1 ttataaatga tactcactct caggggcgtt gcggtttact atgcaggata cggatttttt
       61 cagctggcgt cgcacgatgc tgttgcgttt tcagaggatg gaggccgcag aagaggtcta
      121 ccatgaaata gagcttcagg ctcagcagct ggagtacgat tactattcgt tatgtgttcg
      181 ccacccggta ccattcacgc gacctaaagt ggctttctac accaattacc ctgaggcgtg
      241 ggttagttat tatcaggcaa aaaactttct cgcaattgat ccggtgctga accctgaaaa
      301 ctttagtcag ggccatctaa tgtggaatga tgacttattc aacgaagcac agccgttatg
      361 ggaagccgcg cgcgcacatg gtttacgccg cggtgtcact cagtatttaa tgctgccaaa
      421 ccgggcgctg ggctttttgt ccttttcccg ttgcagcgcg cgcgaaatac ccattcttag
      481 tgatgaactg caattaaaaa tgcagttact ggtgcgcgaa agtctgatgg ctctgatgcg
      541 tttaaatgat gaaatagtga tgacgccaga gatgaatttc agcaagcgcg aaaaagaaat
      601 tctgaggtgg acggcggaag ggaagacatc agcagagata gcgatgattt tgtcaatctc
      661 tgagaatacg gtcaatttcc atcagaaaaa catgcagaaa aaaatcaatg caccgaataa
      721 gacccaggtt gcctgttacg cggccgctac tggcttaatt tgatctcttt tctgtcctgc
      781 gtgccagatg caaaaaccgg ctgaaaggca cgctatcagc cggttttata ttactgacgg
      841 taggcttgtt taatttgctt aaccgttttg gaaaatactg cagcctgggt ttcgtcttcg
      901 atcagcgcaa gctgtttttc catctttaac atcacgcggc ccgcgtcagc ttgtcccatc
      961 gcctgcagca tcagcgtcag catcgctttc aggcaggaga cttcatttgc cagttcttga
     1021 ttattctcgg cagtagaaaa atcaggcgta ctcatttatt ttcctcgtca tgttgcaatg
     1081 aaaatttgcg gtgaataatg ttaaggcggc ggagtatacc ataagctttg ctaaaaatag
     1141 cagtggttgt tttttgagcg tatcgtcaac ggtgctatta aatgcgttaa tataatgacc
     1201 aataaatatt tttatcatga atgttttttg cccgt
//
LOCUS       DAZKHF020000542         1223 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2201S31241
            SAMN43267800-rid25106413.denovo.546, whole genome shotgun sequence.
ACCESSION   DAZKHF020000542 DAZKHF020000000
VERSION     DAZKHF020000542.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267800
            Sequence Read Archive: SRR30307263
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1223)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1223)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 1223)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350581.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350581.2
            Genome Coverage       :: 151x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:21:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,652
            CDSs (total)                      :: 5,557
            Genes (coding)                    :: 5,320
            CDSs (with protein)               :: 5,320
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 237
            CDSs (without protein)            :: 237
            Pseudo Genes (ambiguous residues) :: 0 of 237
            Pseudo Genes (frameshifted)       :: 102 of 237
            Pseudo Genes (incomplete)         :: 130 of 237
            Pseudo Genes (internal stop)      :: 52 of 237
            Pseudo Genes (multiple problems)  :: 41 of 237
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1223
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267800-rid25106413.denovo.546"
                     /serotype="O 26:H11"
                     /isolate="2201S31241"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-01"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            complement(402..1034)
                     /locus_tag="ACHOSC_RS25695"
                     /old_locus_tag="pgaptmp_005139"
     CDS             complement(402..1034)
                     /locus_tag="ACHOSC_RS25695"
                     /old_locus_tag="pgaptmp_005139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfdX family protein"
                     /protein_id="HGU4550878.1"
                     /translation="MKRLIMATMVTAILASSTVWAADNAPVAAQQQTQQVQQTQKTAA
                     AERISEQGLYAMRDVQVARLALFHGDPEKAKELTNEASALLSDDSTEWAKFAKPGKKT
                     NLNDDQYIVINASVGISESYVATPEKEAAIKIANEKMAKGDKKGAMEELRLAGVGVME
                     NQYLMPLKQTRNALADAQKLLDKKQYYEANLALKGAEDGIIVDSEALFVN"
BASE COUNT          324 a          256 c          255 g          388 t
ORIGIN      
        1 aaatcctgta tcaagtgctg aaaatcattt catcgccact tccaattcct ttctgcatgg
       61 taagtagtga ggacgaaaag aagaatatga taaatattga ttcgtggtaa tcagagagag
      121 tcgcgacatt tgtgatgtta ttcttacaac tgtaagaagc gccggtgatt tacaagtaag
      181 ggtatgatta cgctttttaa agcgtaccat aaaacataac ataataaatg atttgtgatg
      241 acacgggatt attgggattt actttgagga agataaaatg cgtgtttata gaaccgcgag
      301 cgccaaataa ttttatgacc acaacctgtc gtattgtggt cataaaaaat tgtcggctgg
      361 agggagttaa atcctttacc tcaatatact gcctgtcagc attagttcac aaacagtgct
      421 tcgctgtcga cgatgattcc atcttccgca cctttaagcg ccaggtttgc ctcgtagtat
      481 tgctttttat caagcagttt ctgcgcatca gcaagtgcat tacgagtctg tttaagcggc
      541 atcagatatt ggttttccat tacgccaaca ccggccaggc gaagttcttc cattgcgccc
      601 tttttatccc ctttcgccat tttttcattg gcaattttta tcgcggcttc tttttcggga
      661 gttgctacat aactctcaga aattccgaca gatgcattaa tgacaatata ttggtcatca
      721 ttgagattgg tctttttacc tggcttagcg aactttgccc attcggtact gtcatctgac
      781 agcaaagcgg aagcttcatt ggtcagttct tttgcctttt ctggatcgcc atgaaacaga
      841 gccaggcgcg ctacctgcac gtcacgcatc gcatataaac cttgttcaga gatacgctct
      901 gctgctgccg ttttctgggt ttgctgaact tgctgagttt gctgttgtgc cgctaccggt
      961 gcattatcag cagcccatac agttgaagat gccagaattg ctgtgaccat cgtagccata
     1021 attaaacgtt tcatacttac ctcattcttt aacagtttta gaaaacgccg ccatcatatt
     1081 gggtgagcat gaaatgagaa atcagagagc aacggtcatt ttgaaagggt tatcttacag
     1141 ttgtagttat tatcgcaaga agtattttag tcttacaatt gtaggaatct gcttcctgtc
     1201 gcagagaact attcttatgt acc
//
LOCUS       DAZKHF020000543         1203 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2201S31241
            SAMN43267800-rid25106413.denovo.547, whole genome shotgun sequence.
ACCESSION   DAZKHF020000543 DAZKHF020000000
VERSION     DAZKHF020000543.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267800
            Sequence Read Archive: SRR30307263
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1203)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1203)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 1203)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350581.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350581.2
            Genome Coverage       :: 151x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:21:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,652
            CDSs (total)                      :: 5,557
            Genes (coding)                    :: 5,320
            CDSs (with protein)               :: 5,320
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 237
            CDSs (without protein)            :: 237
            Pseudo Genes (ambiguous residues) :: 0 of 237
            Pseudo Genes (frameshifted)       :: 102 of 237
            Pseudo Genes (incomplete)         :: 130 of 237
            Pseudo Genes (internal stop)      :: 52 of 237
            Pseudo Genes (multiple problems)  :: 41 of 237
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1203
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267800-rid25106413.denovo.547"
                     /serotype="O 26:H11"
                     /isolate="2201S31241"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-01"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            15..1067
                     /gene="yhiM"
                     /locus_tag="ACHOSC_RS25700"
                     /old_locus_tag="pgaptmp_005140"
     CDS             15..1067
                     /gene="yhiM"
                     /locus_tag="ACHOSC_RS25700"
                     /old_locus_tag="pgaptmp_005140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417948.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2776 domain-containing protein"
                     /protein_id="HGU4550879.1"
                     /translation="MNIYIGWLFKLIPLIMGLICIALGGFVLESSGQSEYFVAGHVLI
                     SLAAICLALFTTAFIIISQLTRGVNTFYNTLFPIIGYAGSIITMIWGWALLAGNDVMA
                     DEFVAGHVIFGVGMIAACVSTVAASSGHFLLIPKNAAGSKSDGTPVQAYSSLIGNCLI
                     AVPVLLTLLGFIWSITLLRSADITPHYVAGHVLLGLTAICACLIGLVATIVHQTRNTF
                     STKEHWLWCYWVIFLGSITVLQGIYVLVSSDASARLAPGIILICLGMICYSIFSKVWL
                     LALVWRRTCSLANRIPMIPVFTCLFCLFLASFLAEMAQTDMGYFIPSRVLVGLGAVCF
                     TLFSIVSILEAGSAKK"
BASE COUNT          269 a          261 c          284 g          389 t
ORIGIN      
        1 ctgaggagtt taaagtgaac atatatatcg ggtggctttt caaattaatc cctttaatta
       61 tgggcttaat ttgtatcgcg ttaggtggct ttgtgctgga aagttctggg caaagtgagt
      121 atttcgttgc gggtcatgtg ctgatttctc tggcagccat atgcctggca ttattcacta
      181 ccgcatttat tatcatttcg cagctcacgc gcggcgttaa tacgttttac aatacattgt
      241 tccccattat tggctatgcg gggtcaatta tcaccatgat atggggttgg gcactgttag
      301 caggcaatga tgtgatggca gacgagtttg tcgccggcca tgttattttc ggcgttggta
      361 tgattgccgc ctgtgtatcg acggtggcag cgtcatccgg tcactttctg ctcattccca
      421 aaaatgcagc ggggagcaag agcgatggaa cgccggtaca ggcttattct tcattaatcg
      481 gtaattgcct cattgccgtt cccgttttac tcaccctgct cggtttcatt tggtctatta
      541 cgctgttacg tagtgctgac ataactccgc attatgtcgc gggtcacgta ttgcttgggt
      601 taaccgcaat ctgtgcctgt ctaattggcc ttgttgccac aattgtccat caaacacgta
      661 atacgttttc aactaaagaa cactggctgt ggtgttattg ggttattttt ctcggctcaa
      721 tcacggtact gcaggggata tacgtcttag tcagttccga tgcaagcgcc cgactggctc
      781 ccggcattat tcttatttgc ctcggaatga tctgttacag catattctca aaagtctggc
      841 tactggcact ggtatggaga cgtacctgtt cgttagccaa cagaataccg atgattcccg
      901 tcttcacctg cctgttttgc cttttcctgg catcgtttct tgcggaaatg gcgcagaccg
      961 acatgggata ttttattcct tcgcgggttt tggtcggttt aggagcggta tgctttacgt
     1021 tgttctcaat cgtttcaata ttagaagcag gttctgctaa aaaataattg caacgtaccg
     1081 gataaaacca gcgttgacca tttgcgtaac gctggttttt cttaggcatc atgaaataac
     1141 gcagcattaa tgcatagtgg ttaaggataa aaaaacaaca aagttactgt ttttttcaac
     1201 ctg
//
LOCUS       DAZKHF020000544         1202 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2201S31241
            SAMN43267800-rid25106413.denovo.548, whole genome shotgun sequence.
ACCESSION   DAZKHF020000544 DAZKHF020000000
VERSION     DAZKHF020000544.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267800
            Sequence Read Archive: SRR30307263
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1202)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1202)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 1202)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350581.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350581.2
            Genome Coverage       :: 151x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:21:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,652
            CDSs (total)                      :: 5,557
            Genes (coding)                    :: 5,320
            CDSs (with protein)               :: 5,320
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 237
            CDSs (without protein)            :: 237
            Pseudo Genes (ambiguous residues) :: 0 of 237
            Pseudo Genes (frameshifted)       :: 102 of 237
            Pseudo Genes (incomplete)         :: 130 of 237
            Pseudo Genes (internal stop)      :: 52 of 237
            Pseudo Genes (multiple problems)  :: 41 of 237
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1202
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267800-rid25106413.denovo.548"
                     /serotype="O 26:H11"
                     /isolate="2201S31241"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-01"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            complement(<1..>1202)
                     /locus_tag="ACHOSC_RS25705"
                     /old_locus_tag="pgaptmp_005141"
     CDS             complement(<1..>1202)
                     /locus_tag="ACHOSC_RS25705"
                     /old_locus_tag="pgaptmp_005141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1983 domain-containing protein"
                     /protein_id="HGU4550880.1"
                     /translation="QAKWDTPKVVKGVSFMLRLTVVADDGSERLVSTARTTETTYRFT
                     QLAPGNYRLTVRAVNAWGQQGDPASVSFRIAAPAAPSQIELTPGYFQITAVPRLAVYD
                     PTVQFEFWFSETRITDIRQVETTARYLGTGLYWIAASINIKPGHDYYFYIRSVNTVGK
                     SAFVEAVGQPSDDASGYLDFFKGEIGKTHLAQELWTQIDNGQLAPDLAEIRTSITDVS
                     NEITQTVNKKLEDQSAAIQQIQKVQVDTNNNLNSMWAVKLQQMKDGRLYIAGIGAGIE
                     NTPAGMQSQVLLAADRIAMINPANGNTKPMFVGQGDQIFMNDVFLKRLTAPTITSGGN
                     PPAFSLTPDGKLTAKNADISGSVNANAGTLNNVTINENCQIKGKLSANQIEGDIVKTV
                     GKAFPRDSR"
BASE COUNT          248 a          358 c          287 g          309 t
ORIGIN      
        1 cgggagtccc gcggaaaagc cttacccact gttttgacta tatcgccttc aatctggttg
       61 gctgacagtt tccccttaat ctgacagttc tcattaattg tgacattgtt gagcgtcccg
      121 gcgttcgcat tcacactgcc actgatatct gcatttttag cggtcagctt tccgtccggt
      181 gtcagggaaa atgccggagg attaccgccg ctggtaatgg tgggagccgt caggcgtttc
      241 aggaacacgt cgttcatgaa tatctgatcg ccctgaccaa caaacatcgg ctttgtgttg
      301 ccattcgcag gattaatcat cgcaatcctg tctgccgcca gcagcacctg actctgcatt
      361 cctgctggcg tattctcaat accggcaccg atacccgcaa tataaaggcg tccgtccttc
      421 atctgttgca gcttcacagc ccacatgctg ttcagattat tatttgtatc aacctgaacc
      481 ttctgtatct gctggatcgc tgcactctgg tcttccagtt tcttattgac ggtctgcgtg
      541 atttcattac tgacatccgt gatggacgtt ctgatttccg ccaggtcagg cgcaagctga
      601 ccgttatcaa tctgagtcca caactcctga gccagatggg ttttccctat ctctcctttg
      661 aaaaaatcca gatagccgga tgcatcatca ctcggctggc caacagcctc cacaaatgcc
      721 gatttgccaa cggtgttcac actgcggata taaaagtaat aatcatggcc cggtttgata
      781 ttgatactgg cggctatcca gtacagcccc gtgccaaggt agcgggctgt ggtttcaacc
      841 tgcctgatat cggtaatccg cgtttccgaa aaccagaact caaactgtac cgtcgggtca
      901 tacaccgcaa gacgcgggac cgctgttatc tgaaaatagc ccggtgtcag ctcaatctgt
      961 gacggcgctg ccggtgcggc aatccggaac gataccgacg ccggatcgcc ctgctgcccc
     1021 cacgcattta ccgcccggac tgtcagcctg tagttccccg gcgccagttg cgtgaagcgg
     1081 tatgtggttt ccgtcgtccg ggccgtgctg accagccgct cactgccgtc atccgcgacc
     1141 acggtcaggc gaagcataaa gctcacgccc ttcaccacct tcggcgtgtc ccatttcgcc
     1201 tg
//
LOCUS       DAZKHF020000545         1193 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2201S31241
            SAMN43267800-rid25106413.denovo.549, whole genome shotgun sequence.
ACCESSION   DAZKHF020000545 DAZKHF020000000
VERSION     DAZKHF020000545.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267800
            Sequence Read Archive: SRR30307263
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1193)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1193)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 1193)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350581.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350581.2
            Genome Coverage       :: 151x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:21:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,652
            CDSs (total)                      :: 5,557
            Genes (coding)                    :: 5,320
            CDSs (with protein)               :: 5,320
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 237
            CDSs (without protein)            :: 237
            Pseudo Genes (ambiguous residues) :: 0 of 237
            Pseudo Genes (frameshifted)       :: 102 of 237
            Pseudo Genes (incomplete)         :: 130 of 237
            Pseudo Genes (internal stop)      :: 52 of 237
            Pseudo Genes (multiple problems)  :: 41 of 237
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1193
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267800-rid25106413.denovo.549"
                     /serotype="O 26:H11"
                     /isolate="2201S31241"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-01"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            179..472
                     /gene="iss"
                     /locus_tag="ACHOSC_RS25710"
                     /old_locus_tag="pgaptmp_005142"
     CDS             179..472
                     /gene="iss"
                     /locus_tag="ACHOSC_RS25710"
                     /old_locus_tag="pgaptmp_005142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="increased serum survival lipoprotein Iss"
                     /protein_id="HGU4550881.1"
                     /translation="MKKMLFSAALAMLITGCAQQTFTVGNKPTAVTPKETITHHFFVS
                     GIGQEKTVDAAKICGGAENVVKTETQQTFVNGLLGFITLGIYTPLEARVYCSQ"
     gene            complement(563..745)
                     /gene="rzoD"
                     /locus_tag="ACHOSC_RS25715"
                     /old_locus_tag="pgaptmp_005143"
     CDS             complement(563..745)
                     /gene="rzoD"
                     /locus_tag="ACHOSC_RS25715"
                     /old_locus_tag="pgaptmp_005143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prophage lysis lipoprotein RzoD"
                     /protein_id="HGU4550882.1"
                     /translation="MRKLKMMLCVMMLPLVVVGCTSKQSVSQCVKPPPPPAWIMQPPP
                     DWQTPLNGIISPSERG"
     gene            complement(962..>1193)
                     /gene="rrrD"
                     /locus_tag="ACHOSC_RS25720"
                     /old_locus_tag="pgaptmp_005144"
     CDS             complement(962..>1193)
                     /gene="rrrD"
                     /locus_tag="ACHOSC_RS25720"
                     /old_locus_tag="pgaptmp_005144"
                     /EC_number="3.2.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="lysozyme RrrD"
                     /protein_id="HGU4550883.1"
                     /translation="IEVDIPETTRGALYSFVYNVGAGNFRTSTLLRKINQGDIKGACD
                     QLRRWTYAGGKQWKGLMTRREIEREICLWGQQ"
BASE COUNT          303 a          285 c          272 g          333 t
ORIGIN      
        1 ctaaaagggt aatggacaga tagagcggtt tatttcattc cacaggattc tgagtgcccc
       61 cctcctccaa taggctgagc atccacctat atagttttaa ttttcatcaa tccatttaac
      121 tatcgtttaa ttgttgtcac ataggattct gccgttttta acaatgcagg ataataagat
      181 gaaaaaaatg ttgttttctg ccgctctggc aatgcttatt acaggatgtg ctcaacagac
      241 gtttactgtt ggaaacaaac cgacagcagt aacaccaaag gaaaccatca cccatcactt
      301 cttcgtttcg ggaattggac aggagaaaac tgttgatgca gccaaaattt gtggcggcgc
      361 agaaaatgtt gttaaaacag aaacccagca aacattcgta aatggattgc tcggttttat
      421 tactttaggc atttatactc cgctggaagc gcgtgtgtat tgctcacaat aattgcatga
      481 gttgcccatc gatatgggca gctctatctg cactgctcat taatatacgt ctgggttcct
      541 tccagttgtt tttgcatagt gatcagcctc tctctgaggg tgaaataatc ccgttcagcg
      601 gtgtctgcca gtcgggggga ggctgcatta tccacgccgg aggcggtggt ggcttcacgc
      661 actgactgac agactgcttt gatgtgcaac cgacgacgac cagcggcaac atcatcacgc
      721 agagcatcat tttcagcttt cgcatcagct aactccttcg tgtattttgc atcgagcgca
      781 gcaacatcac gctgacgcat ctgcatgtca gtaattgccg cgttcgccag cttcagttct
      841 ctggcatttt tgtcgcgctg ggctttgtag gtaatggcgt tatcacggta atgattaaca
      901 gcccatgaca ggcagacgat gatgcagata accagagcgg agataatcgc ggttactctg
      961 ttcattgctg accccacaaa cagatttcac gctcaatctc acgacgagtc atgagacctt
     1021 tccattgctt accgccagca tatgtccagc gacgtagctg atcacatgcg cctttgatat
     1081 cgccctggtt tattttgcga agaagcgtcg atgttctgaa attgccagca cccacgttgt
     1141 aaacgaatga gtaaagagcg ccgcgcgttg tttccggtat atcgacttcg atg
//
LOCUS       DAZKHF020000546         1192 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2201S31241
            SAMN43267800-rid25106413.denovo.550, whole genome shotgun sequence.
ACCESSION   DAZKHF020000546 DAZKHF020000000
VERSION     DAZKHF020000546.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267800
            Sequence Read Archive: SRR30307263
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1192)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1192)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 1192)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350581.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350581.2
            Genome Coverage       :: 151x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:21:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,652
            CDSs (total)                      :: 5,557
            Genes (coding)                    :: 5,320
            CDSs (with protein)               :: 5,320
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 237
            CDSs (without protein)            :: 237
            Pseudo Genes (ambiguous residues) :: 0 of 237
            Pseudo Genes (frameshifted)       :: 102 of 237
            Pseudo Genes (incomplete)         :: 130 of 237
            Pseudo Genes (internal stop)      :: 52 of 237
            Pseudo Genes (multiple problems)  :: 41 of 237
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1192
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267800-rid25106413.denovo.550"
                     /serotype="O 26:H11"
                     /isolate="2201S31241"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-01"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            1..654
                     /locus_tag="ACHOSC_RS25725"
                     /old_locus_tag="pgaptmp_005145"
                     /pseudo
     CDS             1..654
                     /locus_tag="ACHOSC_RS25725"
                     /old_locus_tag="pgaptmp_005145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_086558153.1"
                     /note="programmed frameshift; internal stop; incomplete;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="IS1-like element IS1F family transposase"
     gene            complement(665..736)
                     /locus_tag="ACHOSC_RS25730"
                     /old_locus_tag="pgaptmp_005146"
                     /pseudo
     CDS             complement(665..736)
                     /locus_tag="ACHOSC_RS25730"
                     /old_locus_tag="pgaptmp_005146"
                     /inference="COORDINATES: protein motif:HMM:NF013446.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            799..945
                     /locus_tag="ACHOSC_RS25735"
                     /old_locus_tag="pgaptmp_005147"
                     /pseudo
     CDS             799..945
                     /locus_tag="ACHOSC_RS25735"
                     /old_locus_tag="pgaptmp_005147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612150.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
     gene            972..1076
                     /locus_tag="ACHOSC_RS25740"
                     /old_locus_tag="pgaptmp_005148"
                     /pseudo
     CDS             972..1076
                     /locus_tag="ACHOSC_RS25740"
                     /old_locus_tag="pgaptmp_005148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612087.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
BASE COUNT          335 a          274 c          290 g          293 t
ORIGIN      
        1 aggcgtggtg cgtaacggca aaagcactgc cggacatcag cgctatctct gctctccttg
       61 ccgtaaaaca tggcaactac agttcactta caccgcctct cagcccggta agcaccagaa
      121 aatcattgat atggccatga atggcgtcgg atgtcgcgcc agtgcacgca ttatgggcgt
      181 tggcctcaac acggttttac gtcacttaaa aaaatcaggc cgcagtcggt aacctcgcgc
      241 atacaaccgg gcagtgatgt gattgtctgc gctgaaatgg acgaacagtg gggctacgtc
      301 ggtgctaaat cacgtcagcg ctggctgttt tacgcgtatg acaggatacg gaggacggtt
      361 gtggcgcacg tcttcggtga acgcactctg gccacactgg agcgtcttct gagcctgctg
      421 tcggcctttg aggtcgtggt atagatgacg gatggctggc cgctgtatga atcacgcctg
      481 aagggaaagc tgcacgttat cagcaagcgt tacactcagc gcattgagcg acataatctg
      541 aatctgagac aacatctggc aaggctggga cggaagttac tgtcgttctc aaaatcggtg
      601 gagttgcatg acaaggtcat cgggcattat ctgaacataa aacactatca gtaagttgga
      661 gtcattacct cctcccgaac tcaaagctat catacagata attactggtt aaccctatat
      721 agggcatttt aaagtgttgt gatcttaccc agcaatagtg gacacgcggc taagtgagta
      781 aactctcagt cagaggtgat tcacaagaca aaacagtttc aaccagtaaa aaaacgtaaa
      841 caccattcgc atgaatttcg tagttaagat ctgaagcttg cagaatgcat cggtgttact
      901 gccggagttc gtgaacttag tctgcatgaa tcaccacagc tttacatctg tcgcagtaaa
      961 cagcaaaatc agcaaactta cttaattata tcgagtgcga ttacaatcgc tggcgatggt
     1021 acagttggtg tggcggtctc agtccggaac aatttgaaaa tcagaatctc acttaaaacg
     1081 gcatccattt tacaaaagat ggccgtctgt gtatttgtaa tgaatacctt ctacatgttt
     1141 agagtgcata atccacgaca aggcgatatc ctaaaaatta gctagcaggc aa
//
LOCUS       DAZKHF020000547         1184 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2201S31241
            SAMN43267800-rid25106413.denovo.551, whole genome shotgun sequence.
ACCESSION   DAZKHF020000547 DAZKHF020000000
VERSION     DAZKHF020000547.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267800
            Sequence Read Archive: SRR30307263
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1184)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1184)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 1184)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350581.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350581.2
            Genome Coverage       :: 151x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:21:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,652
            CDSs (total)                      :: 5,557
            Genes (coding)                    :: 5,320
            CDSs (with protein)               :: 5,320
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 237
            CDSs (without protein)            :: 237
            Pseudo Genes (ambiguous residues) :: 0 of 237
            Pseudo Genes (frameshifted)       :: 102 of 237
            Pseudo Genes (incomplete)         :: 130 of 237
            Pseudo Genes (internal stop)      :: 52 of 237
            Pseudo Genes (multiple problems)  :: 41 of 237
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1184
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267800-rid25106413.denovo.551"
                     /serotype="O 26:H11"
                     /isolate="2201S31241"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-01"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            complement(<1..>1184)
                     /locus_tag="ACHOSC_RS25745"
                     /old_locus_tag="pgaptmp_005149"
     CDS             complement(<1..>1184)
                     /locus_tag="ACHOSC_RS25745"
                     /old_locus_tag="pgaptmp_005149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001548650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="beta-L-arabinofuranosidase domain-containing
                     protein"
                     /protein_id="HGU4550884.1"
                     /translation="NGHIGELDKLVPHLITEHKIYGRDRITPRTVSAEDGVIKKQNNP
                     DDNMMQYYWWDSETQSNWKDGYCRSAYLLDNKEWQEKASDLCLELIDTQDDDGYIGIY
                     DSSLKFNCKAENGEFWAQATALRFVLGCYESTRNESLLTSLTQAAQCIMAGYPKETSH
                     PFVVEQGFAGHSHGLTITDVFYRLYEITNNIEYLSYCLWLYEDYSAGCVSEQDLQYKN
                     VIDPGYLLKGHGVHTYEHIRALAIAASQRPQYQTALDIFLSQLKYYLTPSGAPIGDEW
                     ITGRTANATYTGYEYCSVHELFSTYVMLIKLTTNIGLADDAEWLFYNASFGMQHPILH
                     AIMYCKTDNCYEANERKHPDSNQFNTRYKYSPTHEEAAVCCVPNTARTIPAFVENMFQ
                     EN"
BASE COUNT          346 a          248 c          221 g          369 t
ORIGIN      
        1 attttcttga aacatatttt cgacaaaagc cggtatggtt cttgctgtat tcggtacgca
       61 gcagacagca gcctcctcat gagtagggga gtatttatat ctggtattaa actgattact
      121 gtccggatgt ttcctttcat ttgcttcata acaattatca gtcttacagt acattatcgc
      181 gtgaagtatg ggatgctgca tgccaaaact agcattatag aatagccact cagcatcatc
      241 ggccagacca atattagtgg tcagttttat taacataaca taagtgctaa atagttcatg
      301 aacagaacaa tattcatagc ctgtataagt ggcattagct gtgcgtcctg taatccactc
      361 atcaccaatg ggagcgccag atggtgtaag ataatatttc aactgggata aaaaaatatc
      421 taatgcagtt tgatactgcg gccgttggct cgctgcgatg gctaatgccc taatatgctc
      481 atacgtatgt acgccatgac ctttaagaag gtaaccaggg tctatgacat ttttatattg
      541 cagatcttgc tccgacacgc atcctgcgga ataatcttca tacagccaca agcaatatga
      601 gagatattca atgttattgg ttatttcata aaggcggtag aaaacatctg ttattgttaa
      661 tccgtggcta tgtccagcaa aaccctgttc cacaacgaaa ggatgtgacg tttctttagg
      721 gtagccagcc ataatgcatt gtgcggcttg ggtaagagaa gttaataaac tctcattacg
      781 ggtggactca taacatccaa gcacaaaacg caaggcagta gcttgtgccc agaactctcc
      841 attttctgct ttacagttaa attttaatga tgaatcataa attccaatat agccatcatc
      901 atcttgcgta tcgataagtt ccaaacataa atcgctcgct ttttcctgcc attctttatt
      961 atctaacaag taggcactgc ggcaatatcc atccttccaa ttcgattgag tttcactatc
     1021 ccaccaataa tattgcatca tgttatcatc gggattattc tgttttttaa taacgccatc
     1081 ttcagcagat acagtgcgtg gagttatacg atctcttcca tatattttat gctccgtaat
     1141 aagatgggga actaatttat ccagttctcc tatatgaccg ttca
//
LOCUS       DAZKHF020000548         1181 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2201S31241
            SAMN43267800-rid25106413.denovo.552, whole genome shotgun sequence.
ACCESSION   DAZKHF020000548 DAZKHF020000000
VERSION     DAZKHF020000548.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267800
            Sequence Read Archive: SRR30307263
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1181)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1181)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 1181)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350581.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350581.2
            Genome Coverage       :: 151x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:21:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,652
            CDSs (total)                      :: 5,557
            Genes (coding)                    :: 5,320
            CDSs (with protein)               :: 5,320
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 237
            CDSs (without protein)            :: 237
            Pseudo Genes (ambiguous residues) :: 0 of 237
            Pseudo Genes (frameshifted)       :: 102 of 237
            Pseudo Genes (incomplete)         :: 130 of 237
            Pseudo Genes (internal stop)      :: 52 of 237
            Pseudo Genes (multiple problems)  :: 41 of 237
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1181
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267800-rid25106413.denovo.552"
                     /serotype="O 26:H11"
                     /isolate="2201S31241"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-01"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            complement(42..929)
                     /gene="rhtA"
                     /locus_tag="ACHOSC_RS25750"
                     /old_locus_tag="pgaptmp_005150"
     CDS             complement(42..929)
                     /gene="rhtA"
                     /locus_tag="ACHOSC_RS25750"
                     /old_locus_tag="pgaptmp_005150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/homoserine exporter RhtA"
                     /protein_id="HGU4550885.1"
                     /translation="MPGSLRKMPVWLPIIILLVAMASIQGGASLAKSLFPLVGAPGVT
                     ALRLALGTLILIAFFKPWRLRFAKEQRLPLLFYGVSLGGMNYLFYLSIQTVPLGIAVA
                     LEFTGPLAVALFSSRRPVDFVWVVLAVLGLWFLLPLGQDVSHVDLTGCALALGAGACW
                     AIYILSGQRAGAEHGPATVAIGSLIAALIFVPIGALQAGEALWHWSVIPLGLAVAILS
                     TALPYSLEMIALTRLPTRTFGTLMSMEPALAAVSGMIFLGETLTPIQLLALGAIIAAS
                     MGSTLTVRKESKIKELDIN"
BASE COUNT          350 a          300 c          284 g          247 t
ORIGIN      
        1 aaattctgca tggttatgca taaccatgca gaaatgtaaa tttaattaat gtctaattct
       61 tttattttgc tctctttgcg tactgtcagc gtagacccca ttgaagcggc gatgatagcg
      121 ccgagcgcca gcagctgtat gggtgtcagt gtttctccga ggaaaatcat cccggaaacg
      181 gcagccagcg ccggttccat gctcatcagc gtaccaaatg tccgtgttgg caaacgggtg
      241 agggcaatca tttccagcga ataaggcaga gcggtcgaga gaatagcgac agccagaccc
      301 aatggaataa ccgaccagtg ccagagtgct tcaccagcct gaagcgctcc aattggcacg
      361 aaaattaacg ctgcaatcaa cgaaccaatt gccaccgtcg cagggccatg ttccgctcct
      421 gcgcgttgcc cacttaaaat gtaaatagcc caacaagccc cggcccccag tgccagcgca
      481 cagccggtta aatcgacatg ggaaacgtct tgccccagcg gtagcaggaa ccacagacca
      541 agaaccgcca gcacaaccca gacgaaatct accgggcgac gagaagagaa cagcgccacc
      601 gccagtggtc cggtgaactc cagcgccacc gcaataccca gcggtactgt ctgaatagaa
      661 agataaaaaa gataattcat cccacccagc gaaacgccgt aaaacaacag cggtaaccgt
      721 tgctctttgg caaagcgcag tcgccatggc ttaaagaacg cgatgaggat cagcgttcct
      781 aatgccagac gcagcgcagt gacacccggt gcgcccacca gaggaaaaag tgacttagct
      841 aacgaggctc caccctgaat agacgccatg gcaacgagca atatgattat tggtaaccag
      901 accggcattt tacgtaatga accaggcatc ctttctccca caaatatcta gacttaagta
      961 aagcgtggag tgtactggat atacccaatg atggttgagc atttgttgaa aaaattttcc
     1021 cccgttttga ctaaaatgcg ccaggattga tggaatcatt agtctggtga ttaggaataa
     1081 tctggatgaa tgacagggaa aacatgcgta atacttacgc agttctctga aaaagtgatt
     1141 taaatttaga tggatagcgg tgtatggaaa cgttctgtta c
//
LOCUS       DAZKHF020000549         1179 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2201S31241
            SAMN43267800-rid25106413.denovo.553, whole genome shotgun sequence.
ACCESSION   DAZKHF020000549 DAZKHF020000000
VERSION     DAZKHF020000549.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267800
            Sequence Read Archive: SRR30307263
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1179)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1179)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 1179)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350581.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350581.2
            Genome Coverage       :: 151x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:21:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,652
            CDSs (total)                      :: 5,557
            Genes (coding)                    :: 5,320
            CDSs (with protein)               :: 5,320
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 237
            CDSs (without protein)            :: 237
            Pseudo Genes (ambiguous residues) :: 0 of 237
            Pseudo Genes (frameshifted)       :: 102 of 237
            Pseudo Genes (incomplete)         :: 130 of 237
            Pseudo Genes (internal stop)      :: 52 of 237
            Pseudo Genes (multiple problems)  :: 41 of 237
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1179
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267800-rid25106413.denovo.553"
                     /serotype="O 26:H11"
                     /isolate="2201S31241"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-01"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            complement(<1..1115)
                     /locus_tag="ACHOSC_RS25755"
                     /old_locus_tag="pgaptmp_005151"
     CDS             complement(<1..1115)
                     /locus_tag="ACHOSC_RS25755"
                     /old_locus_tag="pgaptmp_005151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_304285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tape measure protein"
                     /protein_id="HGU4550886.1"
                     /translation="MATLRELIIKISANSQSFQSEIQRASRMGSEYYRTLQNGGRQAA
                     AAAREQRRALAELNSQLTEIRGSAVGMAGAFAGAFATGHLISLADEWSSVNARLKQAS
                     QSSDEFSSSQKVLMDISQRTGTAFSDNAALFARSAASMREYGYSADDVLKVTEAISTG
                     LKISGASTAEAGSVITQFSQALAQGVLRGEEFNSVNESGDRIVRALAAGMGVARKDLK
                     AMADDGKLTADKVVPALISQLGVLRDEYAAMPETVSSSITKVENAFMAWVGGANEASG
                     VTKTLSGVLNGVAGQIDNVATAVGALVAVGGARYFGNMASGAMSATAGLVTAARNEVA
                     LAEAQFRGTQIATARARAAVYRAQQAVAAARGTEMQIA"
BASE COUNT          240 a          400 c          286 g          253 t
ORIGIN      
        1 gcaatctgca tctccgtccc gcgggctgcc gccacggcct gctgagcacg gtacacggct
       61 gctcttgccc gcgccgtggc aatctgcgtt cccctgaact gtgcttccgc cagtgcaact
      121 tcattacgtg cagccgtcac aagtcctgcc gtggcagaca tcgctccgga ggccatattg
      181 ccaaagtacc gggcaccccc gacggcaacc agtgccccca cggctgttgc cacattatca
      241 atctgtccgg caacaccgtt cagcacgccg gagagcgttt tcgtcacccc gctggcctca
      301 ttcgcaccgc ccacccaggc cataaaggcg ttttccacct tcgtgatact actggaaacc
      361 gtttccggca tggcggcata ttcatcacgc aataccccca gctggctgat taacgcagga
      421 acgactttat ccgccgtcag tttgccgtcg tccgccatcg ccttaaggtc tttacgggcc
      481 acgcccatac ccgcagccag tgcacgtacg atccggtctc cgctttcatt gaccgaatta
      541 aattcctcac cgcgtaacac accctgtgcc agcgcctggc tgaactgggt gatcaccgag
      601 cccgcctctg ccgtactggc accggagatt ttcagccccg tggaaatggc ctccgtcacc
      661 ttcagcacat catcagcact gtaaccatat tcacgcattg aggctgccga gcgggcaaac
      721 agggccgcat tatctgaaaa tgccgtgccc gtccgctggc tgatatccat cagcactttc
      781 tgtgatgacg aaaattcatc agatgactgc gacgcctgtt tcagtcgggc attcacggaa
      841 ctccactcat ccgccagtga aatcaggtgt ccggtggcaa aggcaccggc aaatgcgcca
      901 gccattccga cagcagaacc gcgaatttcc gtcaactggc tgttcagttc tgccagggca
      961 cgtcgctgct cccgggctgc cgcagcggcc tgacgcccgc cattctgcag ggtccggtaa
     1021 tattcactgc ccatacggga agcccgctgg atctccgact ggaatgactg cgaatttgcc
     1081 gaaattttga taatcagttc acgtaacgtc gccattcacc tttctccggg cgtaaaaaaa
     1141 ccgcctcagc ggttctcatc attcatgact gtgctgcaa
//
LOCUS       DAZKHF020000550         1177 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2201S31241
            SAMN43267800-rid25106413.denovo.554, whole genome shotgun sequence.
ACCESSION   DAZKHF020000550 DAZKHF020000000
VERSION     DAZKHF020000550.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267800
            Sequence Read Archive: SRR30307263
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1177)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1177)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 1177)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350581.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350581.2
            Genome Coverage       :: 151x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:21:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,652
            CDSs (total)                      :: 5,557
            Genes (coding)                    :: 5,320
            CDSs (with protein)               :: 5,320
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 237
            CDSs (without protein)            :: 237
            Pseudo Genes (ambiguous residues) :: 0 of 237
            Pseudo Genes (frameshifted)       :: 102 of 237
            Pseudo Genes (incomplete)         :: 130 of 237
            Pseudo Genes (internal stop)      :: 52 of 237
            Pseudo Genes (multiple problems)  :: 41 of 237
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1177
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267800-rid25106413.denovo.554"
                     /serotype="O 26:H11"
                     /isolate="2201S31241"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-01"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            388..495
                     /locus_tag="ACHOSC_RS25760"
                     /old_locus_tag="pgaptmp_005152"
     CDS             388..495
                     /locus_tag="ACHOSC_RS25760"
                     /old_locus_tag="pgaptmp_005152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin Ldr family
                     protein"
                     /protein_id="HGU4550887.1"
                     /translation="MTLAELGMAFWHDLAAPVIAGILASMIVNWLNKRK"
     gene            871..978
                     /locus_tag="ACHOSC_RS25765"
                     /old_locus_tag="pgaptmp_005153"
     CDS             871..978
                     /locus_tag="ACHOSC_RS25765"
                     /old_locus_tag="pgaptmp_005153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin Ldr family
                     protein"
                     /protein_id="HGU4550888.1"
                     /translation="MTLAELGMAFWHDLAAPVIAGILASMIVNWLNKRK"
     gene            complement(983..>1177)
                     /locus_tag="ACHOSC_RS25770"
                     /old_locus_tag="pgaptmp_005154"
     CDS             complement(983..>1177)
                     /locus_tag="ACHOSC_RS25770"
                     /old_locus_tag="pgaptmp_005154"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4550889.1"
                     /translation="FRLAADSTTVFLFRNPSLTHSVLRIPQNIIRLQPLHFSYEKGRR
                     VSPLRPGPGANCHYGSLTPA"
BASE COUNT          308 a          271 c          358 g          240 t
ORIGIN      
        1 aagcggaagt agcgtgtcat gtgggcgtca ggctgccgta atggcaattt gcgcccggac
       61 caggccgcag gagtgaaact ctgcggcctt tttcgtaaga aaaatgcaac ggttgcaagc
      121 ggataatatt ctgcggaatg cgcagcacag agtgaagatg agacggattc cggaacagaa
      181 aaacagtcgt agaatcagcg tcaagccgga aggattccgg tgaggcgcaa tgttgcgggg
      241 gcttaatccc tggtggcatt ggttgctgga gagagaaaac ccccgcacgt tgcaggtatg
      301 cacctgacaa caccacgggg gccattcttg catccaaaca atacaagaat agccgcgaaa
      361 cgttgtcatt acaatacagg cggctatatg acgctcgcag aactgggcat ggccttctgg
      421 catgatttag cggctccggt cattgctggt attcttgcca gtatgatcgt gaactggctg
      481 aacaagcgga agtaacgtgc catgcgggcg tcaggctgcc gtaatggcaa tttgcgcccg
      541 gatcaggccg caggggtgaa actctgcggc ctttttcgta agaaaaatgc aacggttgca
      601 ggcggataat attctgcgga atgcgcagca cagagtgaag acgtgacgga ttccggaaca
      661 gaaaaacagt cgtagaatca gcgtcaagcc ggaagggttc cggtgaggcg caatgttgcg
      721 ggggctttat ccctggtggc attggttgct ggagagagaa aacccccgca cgttgcaggt
      781 atgcacctga caacatcacg ggggccattc ttgaacctga acaactcaag aatagccgcg
      841 aaacgttgtc attacaacac aggcggctat atgacgctcg cagaactggg catggccttc
      901 tggcatgatt tagcggctcc ggtcattgct ggcattcttg ccagtatgat cgtgaactgg
      961 ctgaacaagc ggaagtaacg tgtcatgcgg gcgtcaggct gccgtaatgg caatttgcgc
     1021 ccggaccagg ccgcaggggt gaaactctgc ggcctttttc gtaagaaaaa tgcaacggtt
     1081 gcaagcggat aatattctgc ggaatgcgca gcacagagtg agtaagtgat ggatttcgga
     1141 acagaaaaac agtcgtagaa tcagcggcaa gccggaa
//
LOCUS       DAZKHF020000551         1167 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2201S31241
            SAMN43267800-rid25106413.denovo.555, whole genome shotgun sequence.
ACCESSION   DAZKHF020000551 DAZKHF020000000
VERSION     DAZKHF020000551.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267800
            Sequence Read Archive: SRR30307263
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1167)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1167)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 1167)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350581.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350581.2
            Genome Coverage       :: 151x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:21:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,652
            CDSs (total)                      :: 5,557
            Genes (coding)                    :: 5,320
            CDSs (with protein)               :: 5,320
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 237
            CDSs (without protein)            :: 237
            Pseudo Genes (ambiguous residues) :: 0 of 237
            Pseudo Genes (frameshifted)       :: 102 of 237
            Pseudo Genes (incomplete)         :: 130 of 237
            Pseudo Genes (internal stop)      :: 52 of 237
            Pseudo Genes (multiple problems)  :: 41 of 237
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1167
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267800-rid25106413.denovo.555"
                     /serotype="O 26:H11"
                     /isolate="2201S31241"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-01"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            complement(149..490)
                     /locus_tag="ACHOSC_RS25775"
                     /old_locus_tag="pgaptmp_005155"
     CDS             complement(149..490)
                     /locus_tag="ACHOSC_RS25775"
                     /old_locus_tag="pgaptmp_005155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001205460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0060567 - negative regulation of
                     termination of DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="antiterminator Q family protein"
                     /protein_id="HGU4550890.1"
                     /translation="MRDMYEVLDRWGAWAAADNSGVDWQPVAAGFKGLLPHGKKTRQQ
                     CDDDEGIMIDSCVARLRKYKPEEYELVIAHFVIGISLRTIAKKQKCSDGTIRKELQTA
                     LGFLDGVLSML"
     gene            complement(508..>1167)
                     /locus_tag="ACHOSC_RS25780"
                     /old_locus_tag="pgaptmp_005156"
     CDS             complement(508..>1167)
                     /locus_tag="ACHOSC_RS25780"
                     /old_locus_tag="pgaptmp_005156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001764249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF968 domain-containing protein"
                     /protein_id="HGU4550891.1"
                     /translation="PGAIRLCWHCDNQLRDQFTERLESMATDNCARWVLSVVRRDLGF
                     DDSHVVTMPELCWWLIRNDLADALPESAARKALRLPKPVVPSVTRESDLVPSVPATSI
                     IQDKAKKVLALKVDPESPESFMLRPKRRRWVNEKYTRWVKTQPCACCGKPADDPHHLI
                     GHGQGGMATKAHDLFVLPLCRKHHDELHADTVAFEEKYGSQLELIFRFIDRALAIGVL
                     A"
BASE COUNT          312 a          326 c          234 g          295 t
ORIGIN      
        1 ttcaactaat ttatatgcat gcttattttg cgtatggctt gcaatactaa cggtatttct
       61 tttattacat tgcctcatca taatcagaca aaatattaaa attttattaa agcaacagag
      121 aaaaaaaggg gggtaattcc ccctaatatt acaacattga aagcacacca tccagaaaac
      181 caagagctgt ttgcaactct tttcttattg tcccatcaga acacttctgc ttctttgcaa
      241 tagttcttag tgagatgccg ataacaaaat gagcaataac caactcatac tcttcgggct
      301 tatatttccg caatcgcgca acacagctgt caatcatgat tccttcatca tcatcgcatt
      361 gttggcgtgt tttctttcca tgaggtagta aacctttaaa ccctgcagca acaggctgcc
      421 agtccacacc actgttatct gctgcagccc atgcacccca gcggtccaat acttcataca
      481 tatcacgcat caactttctc cacaaaatca ggccagcacg ccaattgcca gtgcacgatc
      541 gataaaacga aatatcagct ccagctggga gccatacttc tcttcaaatg ccacggtatc
      601 cgcatgcagc tcgtcgtgat gctttctgca caaaggcaac acaaagaggt catgcgcttt
      661 tgtagccatt ccaccctgac cgtgacctat caggtggtgg ggatcatcag caggttttcc
      721 acaacatgcg cacggctgcg tcttaaccca tcgcgtgtac ttttcattaa cccagcggcg
      781 acgttttggg cgtaacataa aagactccgg cgactccgga tccactttca gcgccagcac
      841 ctttttcgct ttatcctgga tgatgctggt ggcaggaacc gaaggcacaa ggtcactttc
      901 ccgggtgaca gacggcacaa caggcttcgg taatctcagt gccttacggg ctgcactttc
      961 cggtaaggca tccgccagat cattacgaat cagccaccag cacagttccg gcattgtcac
     1021 aacgtgactg tcatcaaaac cgagatcccg acgcacgaca gacaacaccc agcgggcaca
     1081 gttatccgtt gccattgatt ccagccgttc cgtgaactga tcgcgcagct ggttatcgca
     1141 gtgccagcac agacggattg cgcccgg
//
LOCUS       DAZKHF020000552         1162 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2201S31241
            SAMN43267800-rid25106413.denovo.556, whole genome shotgun sequence.
ACCESSION   DAZKHF020000552 DAZKHF020000000
VERSION     DAZKHF020000552.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267800
            Sequence Read Archive: SRR30307263
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1162)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1162)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 1162)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350581.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350581.2
            Genome Coverage       :: 151x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:21:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,652
            CDSs (total)                      :: 5,557
            Genes (coding)                    :: 5,320
            CDSs (with protein)               :: 5,320
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 237
            CDSs (without protein)            :: 237
            Pseudo Genes (ambiguous residues) :: 0 of 237
            Pseudo Genes (frameshifted)       :: 102 of 237
            Pseudo Genes (incomplete)         :: 130 of 237
            Pseudo Genes (internal stop)      :: 52 of 237
            Pseudo Genes (multiple problems)  :: 41 of 237
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1162
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267800-rid25106413.denovo.556"
                     /serotype="O 26:H11"
                     /isolate="2201S31241"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-01"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            142..1116
                     /gene="cysB"
                     /locus_tag="ACHOSC_RS25785"
                     /old_locus_tag="pgaptmp_005157"
     CDS             142..1116
                     /gene="cysB"
                     /locus_tag="ACHOSC_RS25785"
                     /old_locus_tag="pgaptmp_005157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012017585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator CysB"
                     /protein_id="HGU4550892.1"
                     /translation="MKLQQLRYIVEVVNHNLNVSSTAEGLYTSQPGISKQVRMLEDEL
                     GIQIFSRSGKHLTQVTPAGQEIIRIAREVLSKVDAIKSVAGEHTWPDKGSLYIATTHT
                     QARYALPNVIKGFIERYPRVSLHMHQGSPTQIADAVSKGNADFAIATEALHLYEDLVM
                     LPCYHWNRAIVVTPDHPLAGKKAITIEELAQYPLVTYTFGFTGRSELDTAFNRAGLTP
                     RIVFTATDADVIKTYVRLGLGVGVIASMAVDPVADPDLVRVDAHDIFSHSTTKIGFRR
                     STFLRSYMYDFIQRFAPHLTRDVVDAAVALRSNEEIEVMFKDIKLPEK"
BASE COUNT          303 a          273 c          274 g          312 t
ORIGIN      
        1 ttttcttcac acctatacac taaggctata aatgatatag tggttatagt tagcaccttt
       61 tttattatta aatcgtatta gtcacccgcc aggtgtgacg aaaaaacgat gttctgatgg
      121 cgtctaagtg gatggtttaa catgaaatta caacaacttc gctatattgt tgaggtggtc
      181 aatcataacc tgaatgtctc atcaacagcg gaaggacttt acacatcaca acccgggatc
      241 agtaaacaag tcagaatgct ggaagacgag ctaggcattc aaattttttc ccgaagcggc
      301 aagcacctga cgcaggtaac gccagcagga caagaaataa ttcgtatcgc tcgcgaagtc
      361 ctgtcgaaag tcgatgccat aaaatcggtt gccggtgagc acacctggcc ggataaaggc
      421 tcgctgtata tcgccaccac gcatacccag gcacgctacg cgttaccaaa cgttatcaaa
      481 ggctttattg agcgttatcc ccgcgtttct ttgcatatgc accagggctc gccgacacaa
      541 attgctgatg ccgtctctaa aggcaatgct gatttcgcta tcgccacaga agcgctgcat
      601 ctgtatgaag atttagtgat gttaccgtgc taccactgga atcgggctat tgtagtcact
      661 ccggatcacc cgctggcagg caaaaaagcc attaccattg aagaactggc gcaatatccg
      721 ttggtgacat ataccttcgg ctttaccgga cgttcagaac tggatactgc ctttaaccgc
      781 gcagggttaa cgccgcgtat cgtcttcacg gcaacggatg ctgacgtcat taaaacttac
      841 gtccggttag ggctgggggt aggggtcatt gccagcatgg cggtggatcc ggtcgccgat
      901 cccgaccttg tgcgtgttga tgctcacgat atcttcagcc acagtacaac caaaattggt
      961 tttcgccgta gtactttctt gcgcagttat atgtatgatt tcattcagcg ttttgcaccg
     1021 catttaacgc gtgatgtcgt tgatgcggct gtcgcattgc gctctaatga agaaattgag
     1081 gtcatgttta aagatataaa actgccggaa aaataatttc ttgcgttatt ttcggcacct
     1141 tttatgtagc gaaggtgccg ga
//
LOCUS       DAZKHF020000553         1155 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2201S31241
            SAMN43267800-rid25106413.denovo.557, whole genome shotgun sequence.
ACCESSION   DAZKHF020000553 DAZKHF020000000
VERSION     DAZKHF020000553.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267800
            Sequence Read Archive: SRR30307263
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1155)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1155)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 1155)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350581.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350581.2
            Genome Coverage       :: 151x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:21:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,652
            CDSs (total)                      :: 5,557
            Genes (coding)                    :: 5,320
            CDSs (with protein)               :: 5,320
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 237
            CDSs (without protein)            :: 237
            Pseudo Genes (ambiguous residues) :: 0 of 237
            Pseudo Genes (frameshifted)       :: 102 of 237
            Pseudo Genes (incomplete)         :: 130 of 237
            Pseudo Genes (internal stop)      :: 52 of 237
            Pseudo Genes (multiple problems)  :: 41 of 237
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1155
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267800-rid25106413.denovo.557"
                     /serotype="O 26:H11"
                     /isolate="2201S31241"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-01"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            <1..865
                     /locus_tag="ACHOSC_RS25790"
                     /old_locus_tag="pgaptmp_005158"
     CDS             <1..865
                     /locus_tag="ACHOSC_RS25790"
                     /old_locus_tag="pgaptmp_005158"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="S49 family peptidase"
                     /protein_id="HGU4550893.1"
                     /translation="IKPVWALANDMNCSAGQLLASAASRRLVTQTARTGSIGVMMAHS
                     NYGAALEKQGVEITLIYSGSHKVDGNPYSHLPDDVRETLQYRMDATRQMFAQKVSAYT
                     GLSVQAVLDTEAAVYSGQEAIDAGLADELVNSTDAITVMRDALDARKSRLSGGRMTKE
                     TQSTTVSATASQADVTDVVPATEGENASAAQPDVNAQITAAVAAENSRIMGILNCEEA
                     HGREEQARVLAETPGMTVETARRILAAAPQSAQARSDTALDRLMQGAPAPLAAGNPAS
                     DAVNDLLNTPV"
     gene            875..>1155
                     /locus_tag="ACHOSC_RS25795"
                     /old_locus_tag="pgaptmp_005159"
     CDS             875..>1155
                     /locus_tag="ACHOSC_RS25795"
                     /old_locus_tag="pgaptmp_005159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="head decoration protein"
                     /protein_id="HGU4550894.1"
                     /translation="MTSKETFTHYQPLGNSDPAHTATAPGGLSAKAPAMTPLMLDTAT
                     RKLVAWDGTTDGAAVGILAVAADQTSTTLTFYKSGTFRYEDVLWPEAAS"
BASE COUNT          256 a          339 c          356 g          204 t
ORIGIN      
        1 cataaaaccg gtatgggcgc ttgccaacga catgaactgc agtgcaggtc agttgcttgc
       61 cagtgccgcc tcccggcgtc tggtcacgca gaccgcccgg acaggctcca tcggcgtcat
      121 gatggctcac agtaattacg gtgctgcgct ggagaaacag ggtgtggaaa tcacgctgat
      181 ttacagcggc agccataagg tggatggcaa cccctacagc catcttccgg atgacgtccg
      241 ggagacactg cagtaccgga tggacgcaac ccgccagatg tttgcgcaga aggtgtcggc
      301 atataccggc ctgtccgtgc aggctgtgct ggataccgag gctgcagtgt acagcggtca
      361 ggaggccatt gatgccggac tggctgatga acttgttaac agcaccgatg cgatcaccgt
      421 catgcgtgat gcactggatg cacgtaaatc ccgtctctca ggagggcgaa tgaccaaaga
      481 gactcaatca acaactgttt cagccactgc ttcgcaggct gacgttactg acgtggtgcc
      541 agcgacggag ggcgaaaacg ccagcgcggc gcagccggac gtgaacgcgc agatcaccgc
      601 agcggttgcg gcagaaaaca gccgcattat ggggatcctc aactgtgagg aggctcacgg
      661 acgcgaagaa caggcacgcg tgctggcaga aacccccggt atgaccgtgg aaacggcccg
      721 ccgcattctg gccgcagcac cacagagtgc acaggcgcgc agtgacactg cgctggatcg
      781 tctgatgcag ggtgccccgg caccgctggc tgcaggtaac ccggcatctg atgccgttaa
      841 cgatttgctg aacacaccag tgtaagggat gtttatgacg agcaaagaaa cctttaccca
      901 ttaccagccg ctgggcaaca gtgacccggc tcataccgca accgcgcccg gcggattgag
      961 tgcgaaagcg cctgcaatga ccccgctgat gctggacacc gccacccgta agctggttgc
     1021 gtgggatggc accaccgacg gtgctgccgt tggcattctg gcggttgctg ctgaccagac
     1081 cagcaccacg ctgacgttct acaagtccgg cacgttccgt tatgaggatg tgctctggcc
     1141 ggaggctgcc agcga
//
LOCUS       DAZKHF020000554         1155 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2201S31241
            SAMN43267800-rid25106413.denovo.558, whole genome shotgun sequence.
ACCESSION   DAZKHF020000554 DAZKHF020000000
VERSION     DAZKHF020000554.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267800
            Sequence Read Archive: SRR30307263
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1155)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1155)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 1155)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350581.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350581.2
            Genome Coverage       :: 151x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:21:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,652
            CDSs (total)                      :: 5,557
            Genes (coding)                    :: 5,320
            CDSs (with protein)               :: 5,320
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 237
            CDSs (without protein)            :: 237
            Pseudo Genes (ambiguous residues) :: 0 of 237
            Pseudo Genes (frameshifted)       :: 102 of 237
            Pseudo Genes (incomplete)         :: 130 of 237
            Pseudo Genes (internal stop)      :: 52 of 237
            Pseudo Genes (multiple problems)  :: 41 of 237
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1155
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267800-rid25106413.denovo.558"
                     /serotype="O 26:H11"
                     /isolate="2201S31241"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-01"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            complement(27..1151)
                     /locus_tag="ACHOSC_RS25800"
                     /old_locus_tag="pgaptmp_005160"
     CDS             complement(27..1151)
                     /locus_tag="ACHOSC_RS25800"
                     /old_locus_tag="pgaptmp_005160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide disphospho-sugar-binding
                     domain-containing protein"
                     /protein_id="HGU4550895.1"
                     /translation="MRKRILFIGPPLYGLLYPLISLAQAFRVIGHDVVISSAGKFANK
                     AAEAGLVVFDAVPGLDSEAGYRHQEELRKKSNIIGHFSFFSDEMADNLIDFAGKWRPD
                     LIVYPPLDPAGPLVAAKYRIPSVMLAVGFAHTSAHIQMLNRSLSNAYRRHGVSGPLCD
                     LAWIDVAPPSMSILKNAGEPVISMRYIPYNGGAVKETWWDRDSDRKRLLISLGTVKPM
                     VDGLELISWVMDSANEVDADIILQLAINARTGLRKLPSNVRLVDWIPMGVFLNGADGF
                     IHHGGAGNTLTALYSGIPQIVFGEGADRSVNAEIVAKRGCGIIPDKHGLTSDLVNRLL
                     YDDSLRFCSDQVAAEMAEQPSPAEIAEVLMRKLKNNGKQL"
BASE COUNT          349 a          284 c          209 g          313 t
ORIGIN      
        1 ttctcgttga aaatttcagc aatacgctac aattgtttcc cgttgttttt taattttctc
       61 atcaaaacct ctgcaatctc tgcaggactg ggttgttcag ccatttcagc ggctacctga
      121 tctgaacaga agcgtagtga atcatcataa agaaggcgat ttaccaaatc actggtcagt
      181 ccatgcttgt ccggaataat cccacaccca cgcttcgcaa caatttctgc attaacagag
      241 cgatctgcac cttcgccaaa cacaatctgt ggtattccac tatacagcgc tgtcagggta
      301 tttcctgcgc caccgtgatg aataaatcca tcagctccat taaggaatac acccataggt
      361 atccagtcaa ccagacgtac atttgatggt agttttcgta aaccagtacg agcatttatt
      421 gcaagttgca aaatgatatc agcatcaact tcatttgcag aatccataac ccatgaaatc
      481 agctccagac catcaaccat tggttttaca gtgccaaggc tgataagtaa acgtttccga
      541 tcagaatccc tgtcccacca tgtttccttt acagcacctc cgttataagg aatatatctc
      601 attgagataa ccggttctcc agcattttta agaatgctca tacttggggg agcaacatca
      661 atccatgcta aatcacatag tggaccgctg actccatgcc gcctgtaagc attgcttaaa
      721 gaacggttta acatctgaat atgggcagat gtatgcgcga atccaacagc cagcatcact
      781 gaaggaattc tatatttagc agcaaccaat gggcctgccg gatcaagagg gggatagact
      841 attaaatctg gcctccattt tcctgcaaaa tcgatgaggt tatctgccat ttcatcgcta
      901 aaaaaagaga aatgaccaat aatattactt tttttcctca actcttcctg atggcgatat
      961 ccagcctctg aatctaaacc tggaactgca tcaaaaacaa ccagtccagc ttctgctgct
     1021 ttattcgcga atttgccagc gctactaatt actacatcat gtccaattac acgaaaggcc
     1081 tgagccagag aaatcaatgg gtataacaaa ccgtacagcg gtgggccaat aaaaagaata
     1141 cgttttcgca ttaaa
//
LOCUS       DAZKHF020000555         1138 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2201S31241
            SAMN43267800-rid25106413.denovo.559, whole genome shotgun sequence.
ACCESSION   DAZKHF020000555 DAZKHF020000000
VERSION     DAZKHF020000555.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267800
            Sequence Read Archive: SRR30307263
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1138)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1138)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 1138)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350581.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350581.2
            Genome Coverage       :: 151x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:21:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,652
            CDSs (total)                      :: 5,557
            Genes (coding)                    :: 5,320
            CDSs (with protein)               :: 5,320
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 237
            CDSs (without protein)            :: 237
            Pseudo Genes (ambiguous residues) :: 0 of 237
            Pseudo Genes (frameshifted)       :: 102 of 237
            Pseudo Genes (incomplete)         :: 130 of 237
            Pseudo Genes (internal stop)      :: 52 of 237
            Pseudo Genes (multiple problems)  :: 41 of 237
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1138
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267800-rid25106413.denovo.559"
                     /serotype="O 26:H11"
                     /isolate="2201S31241"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-01"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            complement(<1..>1138)
                     /locus_tag="ACHOSC_RS25805"
                     /old_locus_tag="pgaptmp_005161"
     CDS             complement(<1..>1138)
                     /locus_tag="ACHOSC_RS25805"
                     /old_locus_tag="pgaptmp_005161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF6531 domain-containing protein"
                     /protein_id="HGU4550896.1"
                     /translation="QYGGPIVQGSAGVRIGAPTGVACSVCPGGMTSGNPVNPLLGAKV
                     QPGETDLALPGPLPFILSRTYSSYRTKTPAPVGVFGPGWKAPSDIRLQIRDDALILND
                     NGGRSIHFEPLLPGEAVYSRSESMWLVRGGKAAQPDGHTLARLWASLPPDIRLSPHLY
                     LATNSAQGPWWILGWSERVPGAEDLLPAPLPPYRVLTGMADRFGRTLAYRREAAGDLA
                     GEITGVTDGAGREFRLVLTTQAQRAEEARKPHTASLSSPDSPRPLSAPSFPDTLPGTE
                     YGADSGIRLSAVWLMHDPEYPENLPAAPLVCYDWTPRGELAAVYDRSGTQMRHFTYDD
                     KYRGRMVGHRYAGRPEMRYRYDDAGRVVEQLNPAGLSYRYQYEQ"
BASE COUNT          201 a          391 c          349 g          197 t
ORIGIN      
        1 tcctgctcat actggtagcg gtaactcagg cctgccgggt taagttgctc caccacccgc
       61 cccgcatcgt cgtagcggta gcgcatctcc ggcctgcctg cataacggtg ccccaccatc
      121 cggccccggt atttatcatc gtaagtaaag tggcgcatct gcgtaccgct gcggtcatac
      181 accgccgcca gctcaccgcg tggcgtccag tcgtagcaca ccagtggcgc ggcaggtaaa
      241 ttttccgggt attccgggtc gtgcatcagc cacaccgccg aaagacggat accgctgtca
      301 gcgccgtatt ccgtaccggg cagtgtgtcg gggaaggaag gagctgatag agggcgggga
      361 ctgtcagggg aagataacga agcggtgtgc ggtttacggg cctcttccgc ccgctgtgcc
      421 tgtgtggtca gtaccagacg gaactcccgc ccggcaccgt ccgtcacgcc ggtgatttcc
      481 ccggccaggt caccggcggc ctcacgccgg tacgccagcg tccgcccgaa gcggtccgcc
      541 atcccggtaa gcacccggta cggcggcagc ggcgctggca gtaagtcctc agcacccggc
      601 acccgctcag accaccccag tatccaccac ggcccctgtg cgctgttggt cgccaggtaa
      661 agatgcgggc ttaaccggat atccggtggc agcgacgccc acagccgtgc cagcgtgtgg
      721 ccgtccggct gcgctgcctt accgccacgc accagccaca ttgactcact gcggctgtac
      781 accgcctccc ccggcagcag cggctcaaag tgaatgctcc gcccgccgtt gtcgttgagt
      841 atcagtgcat catcgcgtat ctgtaagcgg atatcagaag gcgctttcca gccggggccg
      901 aaaacgccca ccggtgcagg cgtcttcgtc cggtagctgc tgtaggtgcg ggagagaatg
      961 aacggcagcg ggccgggcag cgcaaggtcc gtctcgccgg gctgtacttt tgcaccgagc
     1021 agcgggttca ccgggttgcc cgaggtcatc ccgcccggac acaccgagca cgccacgcca
     1081 gttggcgcgc caattcttac acctgccgaa ccctggacaa tgggaccgcc atactgag
//
LOCUS       DAZKHF020000556         1131 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2201S31241
            SAMN43267800-rid25106413.denovo.560, whole genome shotgun sequence.
ACCESSION   DAZKHF020000556 DAZKHF020000000
VERSION     DAZKHF020000556.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267800
            Sequence Read Archive: SRR30307263
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1131)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1131)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 1131)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350581.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350581.2
            Genome Coverage       :: 151x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:21:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,652
            CDSs (total)                      :: 5,557
            Genes (coding)                    :: 5,320
            CDSs (with protein)               :: 5,320
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 237
            CDSs (without protein)            :: 237
            Pseudo Genes (ambiguous residues) :: 0 of 237
            Pseudo Genes (frameshifted)       :: 102 of 237
            Pseudo Genes (incomplete)         :: 130 of 237
            Pseudo Genes (internal stop)      :: 52 of 237
            Pseudo Genes (multiple problems)  :: 41 of 237
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1131
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267800-rid25106413.denovo.560"
                     /serotype="O 26:H11"
                     /isolate="2201S31241"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-01"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            complement(250..>1131)
                     /locus_tag="ACHOSC_RS25810"
                     /old_locus_tag="pgaptmp_005162"
     CDS             complement(250..>1131)
                     /locus_tag="ACHOSC_RS25810"
                     /old_locus_tag="pgaptmp_005162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HGU4550897.1"
                     /translation="FPFIGNRDITTLNTPDLLIPVRAAEAKQIYEIASRLQQRISAVM
                     RYAVQSGIIRYNPALDMAGALTTVKRQHRPALNLSRLPELLSRIDGYKGQPVTRLAVM
                     LNLLVFIRSSELRYARWSEIDIDNAMWTIPAEREPLPGVKFSHRGSKMRTPHLVPLSK
                     QAVAILTELQTWAGENGLIFTGAHDPRKPISENTVNKALRVMGYDTTQDVCGHGFRAM
                     ACSALIESGLWSRDAVERQMSHQERNGVRAAYIHKAEHLEERRLMLQWWADFLDANRE
                     KGISPFEYANVNNPLRR"
BASE COUNT          282 a          277 c          272 g          300 t
ORIGIN      
        1 actttttccg ttcaggaagc tatatcgatt agtcacatgc cccaaatgga ggatcaatca
       61 aattctgcct aaaaccacac tttaagctcc tgctgagata ctccttctct gtcaacttat
      121 acgccgaatg ctaagtggct atttagaggt aacgggtgat tgcggtgcta tcagccggaa
      181 taaaaaagaa atttattgga gcaggttgct cttccccatg ggaagagcat gtgtgtgaat
      241 agccttctgt caccttctca gcggattgtt aacatttgcg tattcaaacg gactgatccc
      301 cttctcccgg ttcgcatcaa gaaaatcggc ccaccactgt agcatcagtc gacgttcttc
      361 cagatgttct gctttatgga tatacgcagc acgtacacca ttacgctcct gatggctcat
      421 ctgacgttcc acagcatcgc gggaccacaa acctgattca atcaatgcac tgcacgccat
      481 cgcccggaat ccatggccac agacatcctg ggttgtgtca taccccatca ccctcagggc
      541 tttatttaca gtattttcac tgattggttt acgcgggtca tgtgctcccg taaagatcag
      601 accattttca ccagcccatg tctgtagttc tgtcagtatg gctacagcct gtttgctgag
      661 tggcacaaga tgtggtgttc gcatcttgga gccccggtgt gagaatttta cgcctggcag
      721 aggttcgcgt tcggctggaa tagtccacat ggcattgtca atatcaattt cagaccagcg
      781 ggcatatctg agttcactgg aacgaataaa aaccagtaaa ttcagcataa cggcaagccg
      841 ggtgacaggc tggcctttat aaccgtcaat acgcgacaga agttcaggca ggcgtgaaag
      901 attcagagcg gggcgatgct ggcgttttac cgtggtcaat gcaccagcca tatccagagc
      961 cgggttatat ctgatgatgc cagactgtac ggcgtaacgc attacagcag atattctttg
     1021 ctgcagacga ctggcgattt cataaatttg ttttgcttct gcagcacgaa cagggataag
     1081 caggtccggg gtattgagtg ttgtgatatc ccggttgccg ataaagggga a
//
LOCUS       DAZKHF020000557         1127 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2201S31241
            SAMN43267800-rid25106413.denovo.561, whole genome shotgun sequence.
ACCESSION   DAZKHF020000557 DAZKHF020000000
VERSION     DAZKHF020000557.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267800
            Sequence Read Archive: SRR30307263
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1127)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1127)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 1127)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350581.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350581.2
            Genome Coverage       :: 151x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:21:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,652
            CDSs (total)                      :: 5,557
            Genes (coding)                    :: 5,320
            CDSs (with protein)               :: 5,320
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 237
            CDSs (without protein)            :: 237
            Pseudo Genes (ambiguous residues) :: 0 of 237
            Pseudo Genes (frameshifted)       :: 102 of 237
            Pseudo Genes (incomplete)         :: 130 of 237
            Pseudo Genes (internal stop)      :: 52 of 237
            Pseudo Genes (multiple problems)  :: 41 of 237
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1127
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267800-rid25106413.denovo.561"
                     /serotype="O 26:H11"
                     /isolate="2201S31241"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-01"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            <1..779
                     /locus_tag="ACHOSC_RS25815"
                     /old_locus_tag="pgaptmp_005163"
     CDS             <1..779
                     /locus_tag="ACHOSC_RS25815"
                     /old_locus_tag="pgaptmp_005163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001069650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="GTPase family protein"
                     /protein_id="HGU4550898.1"
                     /translation="TRHEPVIGIMGKSGAGKSSLCNALFQGEVTPVSDVHAGTREVRR
                     FRLSGHGHSMVITDLPGVGESRDRDAEYEALYRDILPELDLVLWLIKADDRALSVDEY
                     FWRHILQCGHQQVLFVVTQADKTEPCHEWDMAGIQPSPAQAQNIREKTEAVFRLFRPV
                     HPVVAVSARTGWELDTLVSALMTALPDHAASPLMTRLQDELRTESVRAQAREQFTGAV
                     DRIFDTAESVCVASVVRTALRAVRDTVVSVARAVWNWIFF"
BASE COUNT          247 a          309 c          302 g          269 t
ORIGIN      
        1 tcacccgtca cgaacccgtg attggcatta tgggtaaaag cggggccggt aaatcctcac
       61 tctgtaatgc actgtttcag ggggaggtca ccccggtcag tgatgttcac gccggcaccc
      121 gggaagtacg gcgcttccgt ctgagtggcc acggtcacag catggttatc actgacctgc
      181 ccggtgtggg cgagagccgg gacagggatg cagagtatga ggccctgtac cgtgacattc
      241 tgcctgaact tgacctggta ctgtggctga ttaaagccga tgaccgtgcc ctgtctgtgg
      301 atgagtattt ctggcgacac atcctgcagt gcggacatca gcaggtgctg tttgtggtga
      361 cgcaggccga caaaacggag ccctgccatg aatgggatat ggccggtatt cagccttctc
      421 ctgcacaggc acagaatatt cgcgaaaaaa cggaggcggt attccgtctg ttccggcccg
      481 tacatccggt tgtggccgta tcggcccgca ccggctggga actggatacg ctggtcagtg
      541 cgctcatgac agcgcttccc gaccatgccg ccagtcccct gatgacccga ctgcaggacg
      601 agctgcgcac ggaatctgtc cgcgctcagg cccgtgaaca gtttaccggt gcggtggacc
      661 ggatatttga cacggcggag agcgtctgtg ttgcctctgt tgtccgtacg gccctgcgcg
      721 ctgttcgtga cacggtggtc tctgttgccc gtgcggtatg gaactggatt ttcttctgaa
      781 cctgtcgtga ctgatgccct ccctgactct gagtctgctc acaaaagcac tgttttcgtt
      841 actgtctctc ttgtccgtgc aatagctcaa taatagaata aaacgatcaa catctatttt
      901 atcgatcgtt tatatcgatc gataagctaa taataacctt tgtcagtaac atgcacagat
      961 acgtacagaa agacattcag ggaacaacag aaccacaatt cagaaactcc cacagccgga
     1021 cctccggcac tgtaaccctt tacctgccgg tatccacgtt tgtgggtacc ggctttttta
     1081 ttcaccctca atctaaggaa aagctgatga aacgacatct gaatacc
//
LOCUS       DAZKHF020000558         1122 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2201S31241
            SAMN43267800-rid25106413.denovo.562, whole genome shotgun sequence.
ACCESSION   DAZKHF020000558 DAZKHF020000000
VERSION     DAZKHF020000558.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267800
            Sequence Read Archive: SRR30307263
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1122)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1122)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 1122)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350581.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350581.2
            Genome Coverage       :: 151x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:21:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,652
            CDSs (total)                      :: 5,557
            Genes (coding)                    :: 5,320
            CDSs (with protein)               :: 5,320
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 237
            CDSs (without protein)            :: 237
            Pseudo Genes (ambiguous residues) :: 0 of 237
            Pseudo Genes (frameshifted)       :: 102 of 237
            Pseudo Genes (incomplete)         :: 130 of 237
            Pseudo Genes (internal stop)      :: 52 of 237
            Pseudo Genes (multiple problems)  :: 41 of 237
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1122
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267800-rid25106413.denovo.562"
                     /serotype="O 26:H11"
                     /isolate="2201S31241"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-01"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            66..668
                     /gene="ais"
                     /locus_tag="ACHOSC_RS25820"
                     /old_locus_tag="pgaptmp_005164"
     CDS             66..668
                     /gene="ais"
                     /locus_tag="ACHOSC_RS25820"
                     /old_locus_tag="pgaptmp_005164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide core heptose(II)-phosphate
                     phosphatase Ais"
                     /protein_id="HGU4550899.1"
                     /translation="MLAFCRSSLKSKKYFIILLALAAIAGLGTHAAWSSNGLPRIDNK
                     TLARLAQQHPVVVLFRHAERCDRSTNQCLSDKTGITVKGTQDARELGNAFSADIPDFD
                     LYSSNTVRTIQSATWFSAGKKLTVDKRLLQCGNEIYSAIKDLQSKAPDKNIVIFTHNH
                     CLTYIAKNKRDATFKPDYLDGLVMHVEKGKVYLDGEFVNH"
     gene            complement(707..>1122)
                     /gene="nudI"
                     /locus_tag="ACHOSC_RS25825"
                     /old_locus_tag="pgaptmp_005165"
     CDS             complement(707..>1122)
                     /gene="nudI"
                     /locus_tag="ACHOSC_RS25825"
                     /old_locus_tag="pgaptmp_005165"
                     /EC_number="3.6.1.12"
                     /EC_number="3.6.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016818 - hydrolase activity, acting on
                     acid anhydrides, in phosphorus-containing anhydrides
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="nucleoside triphosphatase NudI"
                     /protein_id="HGU4550900.1"
                     /translation="TIVCPLIQNDGAYLLCKMADDRGVFPGQWALSGGGVESGERIEE
                     ALRREIREELGEQLLLTEITPWTFSDDIRTKTYADGRKEEIYMIYLIFDCVSANREVK
                     INEEFQDYAWVKPEDLVHYDLNVATRKTLRLKGLL"
BASE COUNT          300 a          261 c          241 g          320 t
ORIGIN      
        1 cgccagaaat gtcgctcaat tttacggtaa ttgtctggtt gcgcttgtct ataggtggag
       61 tttacgtgtt agctttttgc cgctcatcat tgaagtcaaa aaaatatttc atcattttac
      121 tggcgctcgc tgcaattgcc ggactgggta ctcatgccgc ctggagtagc aatggtttgc
      181 cacgtatcga caataaaaca ttggccagac tggcacagca gcacccggtt gtcgttttgt
      241 ttcgccatgc tgaacgttgc gatcgttcaa ccaatcaatg cttgtcagat aaaacaggta
      301 ttacggttaa aggtacccag gatgcccgtg aactgggcaa cgcttttagc gctgacatcc
      361 ctgatttcga tctttattcc agtaataccg tccggaccat tcagtcggcg acctggtttt
      421 ccgcgggtaa aaagctgacg gtagataaac gacttcttca gtgcggtaat gagatttata
      481 gtgcaattaa ggacttacaa agcaaagcgc ctgataaaaa tatcgttatt ttcacccata
      541 atcattgcct gacatatatt gctaaaaata agcgtgatgc gacatttaaa cctgattatc
      601 tggatggttt agtcatgcat gtggaaaaag gcaaagttta tctggatggg gaattcgtta
      661 accactaaat tttcaatctg acagccagtg atggctgtca tcattgttaa agaagacctt
      721 tcaaacgtaa cgtttttcgg gtggcaacat ttaaatcata atgcaccaga tcttcaggtt
      781 ttacccacgc gtagtcctga aactcttcgt ttattttcac ttctcggttg gcagaaacgc
      841 agtcaaaaat caggtaaatc atataaatct cttccttgcg accatctgca tacgtcttgg
      901 tgcgaatatc atcgctgaag gtccacggcg tgatttctgt caaaagcagc tgttctccca
      961 gttcttcgcg aatttcgcgg cgtagtgcct cttcaattcg ttcgccagac tccacgccgc
     1021 cacccgaaag cgcccattga ccggggaaaa cgccgcgatc gtcggccatt ttacacagca
     1081 aataagcacc atcattttga atcaaagggc atacaatagt cc
//
LOCUS       DAZKHF020000559         1116 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2201S31241
            SAMN43267800-rid25106413.denovo.563, whole genome shotgun sequence.
ACCESSION   DAZKHF020000559 DAZKHF020000000
VERSION     DAZKHF020000559.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267800
            Sequence Read Archive: SRR30307263
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1116)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1116)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 1116)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350581.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350581.2
            Genome Coverage       :: 151x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:21:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,652
            CDSs (total)                      :: 5,557
            Genes (coding)                    :: 5,320
            CDSs (with protein)               :: 5,320
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 237
            CDSs (without protein)            :: 237
            Pseudo Genes (ambiguous residues) :: 0 of 237
            Pseudo Genes (frameshifted)       :: 102 of 237
            Pseudo Genes (incomplete)         :: 130 of 237
            Pseudo Genes (internal stop)      :: 52 of 237
            Pseudo Genes (multiple problems)  :: 41 of 237
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1116
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267800-rid25106413.denovo.563"
                     /serotype="O 26:H11"
                     /isolate="2201S31241"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-01"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            <1..198
                     /locus_tag="ACHOSC_RS25830"
                     /old_locus_tag="pgaptmp_005166"
     CDS             <1..198
                     /locus_tag="ACHOSC_RS25830"
                     /old_locus_tag="pgaptmp_005166"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4550901.1"
                     /translation="FNNHDGNVIIVNTSSRFELRKKYVGTLKINSGVSTVDPHWPPVS
                     KRIKLIFFSPTLHSFSCIGVS"
     gene            234..>1116
                     /locus_tag="ACHOSC_RS25835"
                     /old_locus_tag="pgaptmp_005167"
     CDS             234..>1116
                     /locus_tag="ACHOSC_RS25835"
                     /old_locus_tag="pgaptmp_005167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000091133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4550902.1"
                     /translation="MSRRKVGNMGRGDFDRLCNASGLIYNSSSEDDSGGWDAIVEFPL
                     LGGIALLNSLNEQPIQCLVQIKSSDGEKKGVQVKLSNMKRFCDTPLPCFFFFARYNKN
                     ANIESAYLVHINSDIMFQVLKRIRQNDIGKKSPLHKLSFTVNYSDEDEIDLTNEYALK
                     NAIEKHIPHGMKKYSEDKLRDLGNVGYNESRYAFNFKVDNNEDYKSLISATLGYPTRI
                     NVKDVRGWDTRFDINFQMEELTAEQAIIEIRSVEAFSTGSIIFEDEKEDVSFKCEFFV
                     SPIALNAPRDLASFRICS"
BASE COUNT          356 a          184 c          234 g          342 t
ORIGIN      
        1 tttaataatc atgatggtaa tgtgattatt gtgaatacga gctcaaggtt tgagctccgg
       61 aaaaaatacg ttgggacgtt aaaaatcaac tcgggagtat ccacagttga tcctcattgg
      121 ccccctgtaa gcaagcgcat caagctaata tttttcagcc cgacacttca tagtttttcc
      181 tgcattgggg taagctaaac ctagtatcgt acatatgaaa gagggatgag tttatgagtc
      241 gaagaaaagt tggtaatatg ggacgtggtg attttgacag gctttgcaat gcatctggct
      301 tgatttacaa cagctcctct gaggatgatt ccggagggtg ggatgccatt gtcgaatttc
      361 ctctgttagg tggtatagcc ttactgaata gtttgaatga acagcctata caatgtttag
      421 tgcagataaa gtcttctgac ggagaaaaga aaggtgtaca ggtcaaactt tctaacatga
      481 aacgtttctg tgacacacct ctaccctgtt ttttcttttt tgcgcgttat aataagaatg
      541 ctaatatcga atcagcttac cttgtccaca ttaatagtga tatcatgttt caggttctga
      601 agcggattcg tcaaaatgat ataggaaaaa aatcaccact tcacaaactt tctttcacgg
      661 taaattactc tgatgaggat gagatagacc tgactaatga atatgcactc aaaaatgcta
      721 ttgaaaaaca tattcctcat ggaatgaaaa aatatagtga agataaattg agagatttag
      781 gtaatgtagg atataacgaa agccgctatg cattcaattt caaagttgat aataatgagg
      841 attataagtc tcttattagt gccacgcttg gatatccgac cagaataaat gtaaaagatg
      901 ttcgcggttg ggatactcga tttgatatta attttcagat ggaagaactg acagctgaac
      961 aagcaataat cgaaataaga tctgttgaag cattctcaac aggctctata atattcgaag
     1021 atgaaaaaga agatgtctct tttaagtgtg aatttttcgt ttctcctatt gcacttaatg
     1081 ctccaaggga tctggcaagc tttagaattt gctcgg
//
LOCUS       DAZKHF020000560         1108 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2201S31241
            SAMN43267800-rid25106413.denovo.564, whole genome shotgun sequence.
ACCESSION   DAZKHF020000560 DAZKHF020000000
VERSION     DAZKHF020000560.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267800
            Sequence Read Archive: SRR30307263
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1108)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1108)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 1108)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350581.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350581.2
            Genome Coverage       :: 151x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:21:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,652
            CDSs (total)                      :: 5,557
            Genes (coding)                    :: 5,320
            CDSs (with protein)               :: 5,320
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 237
            CDSs (without protein)            :: 237
            Pseudo Genes (ambiguous residues) :: 0 of 237
            Pseudo Genes (frameshifted)       :: 102 of 237
            Pseudo Genes (incomplete)         :: 130 of 237
            Pseudo Genes (internal stop)      :: 52 of 237
            Pseudo Genes (multiple problems)  :: 41 of 237
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1108
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267800-rid25106413.denovo.564"
                     /serotype="O 26:H11"
                     /isolate="2201S31241"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-01"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            complement(<1..1040)
                     /locus_tag="ACHOSC_RS25840"
                     /old_locus_tag="pgaptmp_005168"
     CDS             complement(<1..1040)
                     /locus_tag="ACHOSC_RS25840"
                     /old_locus_tag="pgaptmp_005168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tape measure protein"
                     /protein_id="HGU4550903.1"
                     /translation="MATLRELIIKISANSQSFQSEIQRASRMGSEYYRTLQNGGRQAA
                     AAAREQRRALAELHSQLTEIRASAVGMTGAFAGAFATGHLISLADEWSSVNARLKQAS
                     QSSDEFASSQKVLMDISQRTGTAFSDNAALFARSAASMREYGYSADDVLKVTEAISTG
                     LKISGASTAEAGSVITQFSQALAQGVLRGEEFNSVNESGDRIVRALAAGMGVARKDLK
                     AMADDGKLTADKVVPALISQLGILRDEYAAMPETVSSSITKVENAFMAWVGGANEASG
                     VTKTLSGMLNGVAGQIDNVATAVGALVAVGVVRYFGNMASGAMSATAGLVTAARNEVA
                     LAEAQFRGAQIAT"
BASE COUNT          241 a          367 c          257 g          243 t
ORIGIN      
        1 gtggcaatct gcgctcccct gaactgtgct tccgccagtg caacttcatt acgtgcagcc
       61 gtcacaagtc ctgccgtggc agacatcgct ccggaggcca tattgccaaa gtaccggaca
      121 accccgacgg caaccagcgc gcccacggct gttgccacat tatcaatctg tccggcaaca
      181 ccgttcagca tgccggagag cgtttttgtc accccgctgg cctcattcgc accgcccacc
      241 caggccataa aggcgttttc cacctttgtg atactactgg aaaccgtttc cggcatggcc
      301 gcatattcat cacgtaatat ccccagctgg ctgattaacg cggggaccac tttatccgct
      361 gtcagttttc cgtcatccgc cattgccttc agatctttac gggccacgcc catacccgca
      421 gccagtgcac gtacgatccg gtcaccactt tcattgaccg aattaaactc ctcaccacgc
      481 aatacaccct gcgccagcgc ctgactgaac tgggtgatca ccgagcccgc ctctgccgta
      541 ctggcaccgg agattttcag ccctgtcgaa atggcctccg tcaccttcag cacatcatca
      601 gcactgtaac catattcacg catcgaggca gccgaacggg caaacagggc cgcattatcc
      661 gaaaatgcgg tgcctgtccg ctggctgata tccatcagca ctttctgtga tgacgcaaat
      721 tcatccgatg actgcgacgc ctgtttcaga cgggcattca cggagctcca ttcatccgcc
      781 agcgaaatca ggtgtccggt ggcaaaggca ccggcaaacg caccggtcat tccgacagcc
      841 gaagcgcgga tttccgtcaa ctggctgtgc agctcagcca gagcccggcg ctgctccctg
      901 gctgccgcag cagcctgacg tccgccattc tgcagggtcc ggtaatattc actgcccata
      961 cgggacgccc gctggatctc cgactggaat gactgtgaat ttgccgaaat tttgataatc
     1021 agttcacgta acgtcgccat tcacctttct ccgggcaaaa aaaacctgcc acagcaggtt
     1081 ttcatcatta tttatgacat tgctgcaa
//
LOCUS       DAZKHF020000561         1104 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2201S31241
            SAMN43267800-rid25106413.denovo.565, whole genome shotgun sequence.
ACCESSION   DAZKHF020000561 DAZKHF020000000
VERSION     DAZKHF020000561.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267800
            Sequence Read Archive: SRR30307263
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1104)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1104)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 1104)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350581.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350581.2
            Genome Coverage       :: 151x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:21:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,652
            CDSs (total)                      :: 5,557
            Genes (coding)                    :: 5,320
            CDSs (with protein)               :: 5,320
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 237
            CDSs (without protein)            :: 237
            Pseudo Genes (ambiguous residues) :: 0 of 237
            Pseudo Genes (frameshifted)       :: 102 of 237
            Pseudo Genes (incomplete)         :: 130 of 237
            Pseudo Genes (internal stop)      :: 52 of 237
            Pseudo Genes (multiple problems)  :: 41 of 237
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1104
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267800-rid25106413.denovo.565"
                     /serotype="O 26:H11"
                     /isolate="2201S31241"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-01"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            58..>1104
                     /locus_tag="ACHOSC_RS25845"
                     /old_locus_tag="pgaptmp_005169"
     CDS             58..>1104
                     /locus_tag="ACHOSC_RS25845"
                     /old_locus_tag="pgaptmp_005169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416505.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YeeR"
                     /protein_id="HGU4550904.1"
                     /translation="MLQIVGALILLIAGFAILRLLFRALISTASALAGLILLCLFGPA
                     LLAGYITERITRLFHIRWLAGVFLTIAGMIISFMWGLDGKHIALEAHTFDSVKFILTT
                     ALAGGLLAVPLQIKNIQQNGITPEDISKEINGYYCCFYTAFFLMACSACAPLIALQYD
                     ISPSLMWWGGLLYWLAALVTLLWAASQIQALKKLTCAISQTLEEQPVLNSKSWLTSLQ
                     NDYSLPDSLTERIWLTLISQRISRGELREFELADGNWLLNNAWYERNMAGFNEQLKEN
                     LSFTPDELKTLFRNRLNLSPEANDDFLDRCLDGGDWYPFSEGRRFVSFHHVDELRICA
                     SCGLTEVHHAPENHK"
BASE COUNT          260 a          275 c          279 g          290 t
ORIGIN      
        1 cttcaacgcc acattcgctg gcattaacaa taacatgata ttcatcacgg agtgactatg
       61 ttacagatag tcggcgcgct gatcctgctg atcgcaggat ttgccattct tcgccttttg
      121 ttcagagcat taatcagcac ggcttctgcg ctggcagggc tcatattgct gtgtctgttc
      181 ggcccggcct tactggctgg ctatatcacc gaacgcataa cccggttgtt ccatattcgc
      241 tggctggcag gcgtatttct gacgattgcc ggaatgatca tcagcttcat gtggggactt
      301 gatggtaaac atatcgcgct ggaggctcac acctttgact ctgtgaaatt tattctgacc
      361 accgctctcg ccggtggtct gctggctgtt cccctgcaga tcaaaaacat tcagcagaac
      421 gggatcacac cagaagatat cagcaaggaa attaacgggt attactgctg tttttatact
      481 gcctttttcc ttatggcgtg ttctgcatgc gcaccattga tcgcgttaca gtacgatatt
      541 tcaccgtcac tgatgtggtg gggcgggttg ttgtactggc tggctgcatt agtgacgctg
      601 ctatgggcgg ccagccagat ccaggcgctg aaaaaactga cctgtgccat cagccagaca
      661 ctggaagaac aaccggtgct caacagtaaa tcgtggctga ccagtttgca aaacgattac
      721 agccttcctg actcactgac ggagcgcatc tggctgacgc tcatttctca acggatttcc
      781 cggggagagc tgagggaatt tgaactggca gacggaaact ggttactgaa caatgcctgg
      841 tatgaaagaa acatggcagg gtttaacgaa cagttgaaag agaacctgtc attcacacct
      901 gatgaactga aaacgctctt ccggaaccgc ctgaatttat caccggaagc gaatgacgat
      961 tttctcgatc gttgcctgga cggcggtgac tggtatccct tttcagaagg tcgccgtttt
     1021 gtatcattcc atcacgtgga tgagcttcgt atctgtgcct cctgcgggct gacagaagta
     1081 catcatgccc cggaaaatca taag
//
LOCUS       DAZKHF020000562         1088 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2201S31241
            SAMN43267800-rid25106413.denovo.566, whole genome shotgun sequence.
ACCESSION   DAZKHF020000562 DAZKHF020000000
VERSION     DAZKHF020000562.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267800
            Sequence Read Archive: SRR30307263
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1088)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1088)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 1088)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350581.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350581.2
            Genome Coverage       :: 151x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:21:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,652
            CDSs (total)                      :: 5,557
            Genes (coding)                    :: 5,320
            CDSs (with protein)               :: 5,320
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 237
            CDSs (without protein)            :: 237
            Pseudo Genes (ambiguous residues) :: 0 of 237
            Pseudo Genes (frameshifted)       :: 102 of 237
            Pseudo Genes (incomplete)         :: 130 of 237
            Pseudo Genes (internal stop)      :: 52 of 237
            Pseudo Genes (multiple problems)  :: 41 of 237
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1088
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267800-rid25106413.denovo.566"
                     /serotype="O 26:H11"
                     /isolate="2201S31241"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-01"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            2..1088
                     /locus_tag="ACHOSC_RS25850"
                     /old_locus_tag="pgaptmp_005170"
                     /pseudo
     CDS             2..1088
                     /locus_tag="ACHOSC_RS25850"
                     /old_locus_tag="pgaptmp_005170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000027427.1"
                     /note="frameshifted; internal stop; incomplete; missing
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ISAs1 family transposase"
BASE COUNT          387 a          151 c          263 g          287 t
ORIGIN      
        1 tttgcttgat tatatttcag tgatccctga tatacgacaa caaggaaagg ttaaacataa
       61 attatctgct attttgtttc tcaccgtatg tgcagtaatt gcaggtgccg atgagtggca
      121 ggaaattgaa gattttggac atgaaagact tgaatggcta aagaaatatg gtgattttga
      181 taatggcatt ccggtcgatg acaccattgc acgcgttgtg agtaacattg acagtttggc
      241 ctttgaaaag atgtttattg aatggatgca ggagtgccat gaaatcactg atggtgaaat
      301 tatagcaata gatggaaaga ccataagagg ctcctttgat aagggaaaaa gaaaaggagc
      361 aatccatatg gtgagtgcat tctcgaacga aaatggtgtt gtactggggc aggtgaaaac
      421 ggaagccaaa agtaatgaga ttacagccat tccagagttg cttaacctac tgtatttaaa
      481 gaaaaatttg ataaccattg atgctatggg ctgtcagaaa gatatcgctt cgaagatcaa
      541 agataaaaaa gcagattatc ttctggcagt aaaaggcaat caggggaaat tacatcatgc
      601 attcgaggaa aaatttcctg taaatgtgtt ttctaattat aaaggcgatt cgtttagtac
      661 gcaggagata agtcatggaa gaaaagaaac acgtttgcat attgtcagta acgtaacgcc
      721 tgaacttttg tgattttgaa ttcgaatgga agggattaaa aaagctttgt gtagcattgt
      781 cattcaggca gaagaaagaa gataaatcag cagaaggtgt aagcatccga tattatattt
      841 catcaaagga tatggatgct aaagaatttg cacatgctat cagagcgcac tggctgatcg
      901 agcacagtct tcattgggtg ttagatgtaa aaatgaatga agatgccagc cggataagaa
      961 gaggaaacgc agcctaaata atatctggaa taaagaagat ggcactgaat ttattaaggg
     1021 attgcaaaga cattaagggt gaagaggaaa agaaagaagg ctgcgttaaa cacagggaaa
     1081 gatcatca
//
LOCUS       DAZKHF020000563         1087 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2201S31241
            SAMN43267800-rid25106413.denovo.567, whole genome shotgun sequence.
ACCESSION   DAZKHF020000563 DAZKHF020000000
VERSION     DAZKHF020000563.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267800
            Sequence Read Archive: SRR30307263
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1087)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1087)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 1087)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350581.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350581.2
            Genome Coverage       :: 151x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:21:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,652
            CDSs (total)                      :: 5,557
            Genes (coding)                    :: 5,320
            CDSs (with protein)               :: 5,320
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 237
            CDSs (without protein)            :: 237
            Pseudo Genes (ambiguous residues) :: 0 of 237
            Pseudo Genes (frameshifted)       :: 102 of 237
            Pseudo Genes (incomplete)         :: 130 of 237
            Pseudo Genes (internal stop)      :: 52 of 237
            Pseudo Genes (multiple problems)  :: 41 of 237
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1087
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267800-rid25106413.denovo.567"
                     /serotype="O 26:H11"
                     /isolate="2201S31241"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-01"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            21..224
                     /locus_tag="ACHOSC_RS25855"
                     /old_locus_tag="pgaptmp_005171"
     CDS             21..224
                     /locus_tag="ACHOSC_RS25855"
                     /old_locus_tag="pgaptmp_005171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail fiber C-terminal domain-containing
                     protein"
                     /protein_id="HGU4550905.1"
                     /translation="MQSLTAQLRLGPADILESDENGIIPEQDRVITQVVILDTDKKLI
                     QCVVRPLQILRADGTWENIGGMK"
     gene            330..>1087
                     /gene="nleH"
                     /locus_tag="ACHOSC_RS25860"
                     /old_locus_tag="pgaptmp_005172"
     CDS             330..>1087
                     /gene="nleH"
                     /locus_tag="ACHOSC_RS25860"
                     /old_locus_tag="pgaptmp_005172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system effector kinase NleH"
                     /protein_id="HGU4550906.1"
                     /translation="MLSPSSVNLGCSWNSLTRNLTSPDSRILSSVRDAAASSDNGAQV
                     KVGNRTYRVVVTDNKFCVTRESHSGCFTNLLHRLGWPKGEISRKIEVMLNSSPVNRAM
                     ERGAVHSNRPDLPPVDYAPPELPSVDYNSLPVPGNVIGKGGNAVVYEDAEDATKVLKM
                     FTTSQSNEEVTNEVRCFNQYYGAGSAEKIYGDNGDIIGIRMDKINGESLLNISSLPAQ
                     AEHAIYDMFDRLEQKGILFIDTTETNVLYDRTRN"
BASE COUNT          320 a          186 c          287 g          294 t
ORIGIN      
        1 agcatgatgg ccgtgaacac gtgcagtcgc ttacagcaca actgcgactg gggccggcag
       61 acatcctgga gtcagatgag aatggcatta ttccggagca ggacagggta atcacacagg
      121 tggtgatact ggatacagat aaaaagctga tacagtgtgt ggtaagaccg ctgcaaatcc
      181 tgcgtgctga cgggacgtgg gaaaatattg gcgggatgaa gtaacccgac agcttcacaa
      241 aaccggagtc cggctccggt ttttgttgtc atgtcatggt gatgtttgtt aagaaagtaa
      301 agattgattc attttgaagg ttgaaatgta tgctatcacc atcttctgta aatttggggt
      361 gttcatggaa ttctttaacc agaaacctga cttctcctga cagtcgtatt ttatcctctg
      421 taagggatgc agctgctagc tcggataatg gggcgcaagt aaaggtgggc aacagaacat
      481 atcgtgttgt tgtcactgat aataagtttt gcgttacaag agaaagtcat agtggttgct
      541 ttactaatct gttgcacagg ctgggatggc ctaagggaga gattagcagg aaaattgagg
      601 tgatgctgaa ttcatcgcca gtgaataggg ctatggaaag aggtgctgtt cattcgaata
      661 gacctgattt acctcctgtt gattatgcac cgccagagtt accgagtgtg gactataaca
      721 gcttgcctgt gcctggtaat gttattggca aagggggtaa cgctgtagta tatgaagatg
      781 ctgaggatgc aacaaaagtc ctgaagatgt ttactacatc tcaaagcaat gaagaggtaa
      841 caaacgaagt tcgttgcttc aaccaatatt atggtgccgg gagtgcagaa aaaatatatg
      901 gcgataatgg cgatattatt ggtattagaa tggataaaat aaatggagaa tcgcttttaa
      961 atatttcgtc cttgccggca caggctgagc atgccattta tgatatgttt gacaggcttg
     1021 agcaaaaagg aattcttttt atcgatacaa cagagacaaa tgtcttatat gatcgcacga
     1081 gaaatga
//
LOCUS       DAZKHF020000564         1083 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2201S31241
            SAMN43267800-rid25106413.denovo.568, whole genome shotgun sequence.
ACCESSION   DAZKHF020000564 DAZKHF020000000
VERSION     DAZKHF020000564.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267800
            Sequence Read Archive: SRR30307263
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1083)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1083)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 1083)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350581.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350581.2
            Genome Coverage       :: 151x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:21:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,652
            CDSs (total)                      :: 5,557
            Genes (coding)                    :: 5,320
            CDSs (with protein)               :: 5,320
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 237
            CDSs (without protein)            :: 237
            Pseudo Genes (ambiguous residues) :: 0 of 237
            Pseudo Genes (frameshifted)       :: 102 of 237
            Pseudo Genes (incomplete)         :: 130 of 237
            Pseudo Genes (internal stop)      :: 52 of 237
            Pseudo Genes (multiple problems)  :: 41 of 237
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1083
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267800-rid25106413.denovo.568"
                     /serotype="O 26:H11"
                     /isolate="2201S31241"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-01"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            complement(<1..473)
                     /locus_tag="ACHOSC_RS25865"
                     /old_locus_tag="pgaptmp_005173"
     CDS             complement(<1..473)
                     /locus_tag="ACHOSC_RS25865"
                     /old_locus_tag="pgaptmp_005173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1737 domain-containing protein"
                     /protein_id="HGU4550907.1"
                     /translation="MAFKHYDVVRAASPSDLAEKLTHKLKEGWQPYGGPVAITPYTLM
                     QAVAIEGDPQVGPSSEPDWFYVVVLAGQSNGMAYGEGLPLPDSYDAPDPRIKQLARRS
                     TVTPGGESCTYNDIIPADHCLHDVQDMSTLNHPKADLSKGQYGCVGQGLHIAKKLL"
     gene            742..858
                     /locus_tag="ACHOSC_RS25870"
                     /old_locus_tag="pgaptmp_005174"
                     /pseudo
     CDS             742..858
                     /locus_tag="ACHOSC_RS25870"
                     /old_locus_tag="pgaptmp_005174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013009199.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(963..1039)
                     /locus_tag="ACHOSC_RS25875"
                     /old_locus_tag="pgaptmp_005175"
     tRNA            complement(963..1039)
                     /locus_tag="ACHOSC_RS25875"
                     /old_locus_tag="pgaptmp_005175"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1003..1005),aa:Arg,seq:tct)
BASE COUNT          255 a          301 c          282 g          245 t
ORIGIN      
        1 agcagttttt tggcaatatg taagccctgg ccgacacagc cgtactgccc tttgctcagg
       61 tctgccttcg gatgattcag cgtactcata tcctgcacat catgcaggca gtggtcagcc
      121 ggaatgatgt cgttatacgt acaactctct ccacccggag ttaccgtgct gcggcgcgcc
      181 agctgtttaa tgcgcggatc cggagcatcg taagaatccg gtaacggaag cccttcaccg
      241 taggccatgc cgttggactg tccggcaagc acaaccacgt agaaccagtc cggctcagat
      301 gaagggccga cctgtggatc tccttcaata gccaccgcct gcatcagtgt gtacggcgta
      361 atggcaaccg gtccgccgta tggctgccag ccctctttca gtttgtgtgt cagcttttcc
      421 gcaaggtctg acggcgacgc cgccctgaca acatcatagt gtttaaatgc catgaatcct
      481 cccggccggg ataatattgt gagtaaaatg aggagcgggc tgaagtccgg aagttacagg
      541 acaatggcag aagagagaca acagcccgca atacgaaaaa ggccgcgcta ttgcgcagag
      601 tgattactgt cggatattat tcgccagctg aaatattact tcacgttttg ttgtttattc
      661 cttgccgccc gcgtctccct gcgcgggctt tttttgtcca taagaaagcc cctccggaga
      721 ggggctggag agtggcgcta tgtgccattg catggtgccg ggtgcctccc ggtgaattca
      781 gtaccagcac ctgaatccgc gattatccca tatacctact cgctgattgc ccctccgcac
      841 agggggattc accatgccag tttcttttaa caaactcccc gcaaaccaga caacagtcaa
      901 ccgcctgaat tgtgagacat ttaaaaaaaa ggcccgcaaa agcgagccag ggaaaataag
      961 tgtggcgcgt tgtactggat tcgaaccagt gaccgattgc ttagaaggca attgctctgt
     1021 ccggctgagc taacaacgca ggatacagat aatggaccgc cttcggggac ccgaactccg
     1081 cgc
//
LOCUS       DAZKHF020000565         1083 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2201S31241
            SAMN43267800-rid25106413.denovo.569, whole genome shotgun sequence.
ACCESSION   DAZKHF020000565 DAZKHF020000000
VERSION     DAZKHF020000565.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267800
            Sequence Read Archive: SRR30307263
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1083)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1083)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 1083)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350581.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350581.2
            Genome Coverage       :: 151x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:21:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,652
            CDSs (total)                      :: 5,557
            Genes (coding)                    :: 5,320
            CDSs (with protein)               :: 5,320
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 237
            CDSs (without protein)            :: 237
            Pseudo Genes (ambiguous residues) :: 0 of 237
            Pseudo Genes (frameshifted)       :: 102 of 237
            Pseudo Genes (incomplete)         :: 130 of 237
            Pseudo Genes (internal stop)      :: 52 of 237
            Pseudo Genes (multiple problems)  :: 41 of 237
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1083
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267800-rid25106413.denovo.569"
                     /serotype="O 26:H11"
                     /isolate="2201S31241"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-01"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            <1..621
                     /gene="traT"
                     /locus_tag="ACHOSC_RS25880"
                     /old_locus_tag="pgaptmp_005176"
     CDS             <1..621
                     /gene="traT"
                     /locus_tag="ACHOSC_RS25880"
                     /old_locus_tag="pgaptmp_005176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_490587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="complement resistance protein TraT"
                     /protein_id="HGU4550908.1"
                     /translation="QYTSTIWLEPASERTVFLQIKNTSDKDMSGLQGKIADAVKARGY
                     QIMTSPDKAYYWIQANVLKADKMDLRESQGWLSRGYEGAAVGAALGGGITAYNTGSAG
                     TTLGVGLATGLIGMAADAMVEDINYTMITDVQIAERTRTSVRTDNVAALRQGTSGSKI
                     QTSTETGNQHKYQTRVVSSANQVNLKFEEAKPHLEDQLAKSIANIL"
     gene            complement(669..920)
                     /locus_tag="ACHOSC_RS25885"
                     /old_locus_tag="pgaptmp_005177"
     CDS             complement(669..920)
                     /locus_tag="ACHOSC_RS25885"
                     /old_locus_tag="pgaptmp_005177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease"
                     /protein_id="HGU4550909.1"
                     /translation="MESFVQDSPFYSGRDLYWLRPKVELTLEEKLYYCSCIRRNRHKY
                     SYGRQANRTLKNLLVPSLDSVPAWVYGVTGKIISELSER"
     gene            complement(944..>1083)
                     /locus_tag="ACHOSC_RS25890"
                     /old_locus_tag="pgaptmp_005178"
     CDS             complement(944..>1083)
                     /locus_tag="ACHOSC_RS25890"
                     /old_locus_tag="pgaptmp_005178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="restriction endonuclease"
                     /protein_id="HGU4550910.1"
                     /translation="GLNLELNKLEKDSSGINFVSRTSKNNGVSARVKLKDGISPCQQL
                     C"
BASE COUNT          315 a          262 c          234 g          272 t
ORIGIN      
        1 cagtacacct caaccatctg gcttgaacct gcgagcgaac gcaccgtttt cctgcagatc
       61 aaaaacacgt cggataaaga catgagtgga ctacagggaa aaattgccga tgcagtcaaa
      121 gccagaggtt atcagatcat gacttccccg gataaagctt actactggat ccaggctaac
      181 gttctcaaag cagacaaaat ggatctgcgt gaatctcagg gatggctgag tcgtggttat
      241 gaaggtgcag cagtcggagc tgctcttgga ggtggaatca cagcgtacaa taccgggtcg
      301 gcaggtacca ctctcggtgt tggactggct accggtctga ttggcatggc cgcggatgca
      361 atggtggaag atataaatta cacaatgatt acagatgttc agattgccga gcgaaccaga
      421 accagcgtca gaacggataa tgttgcagct ttgcgtcagg gaacatcagg ctccaaaatt
      481 cagacaagca cagaaaccgg caaccagcat aaataccaga cccgggttgt atccagtgcc
      541 aaccaggtta atctgaagtt tgaagaagct aaacctcatc tggaagacca gctggcaaaa
      601 tcaatcgcca atatactctg atataaatat cccggtggcc attcacctgg cctccggccc
      661 tcttagtctc aacgttcaga caattcagat attattttcc ctgtcacacc atatacccat
      721 gccggaacag aatctaatga cggtaccagt aaattcttca gcgttcggtt tgcctgacgc
      781 ccgtagctgt atttgtgtct gtttcttctt atacaagagc agtagtacaa tttctcttcc
      841 agagtcagtt caactttagg acgtaaccaa taaagatctc ttccactata aaatggtgaa
      901 tcctgtacaa acgattccat aacagaacct cctgctgcta cagttaacac agctgctggc
      961 atggactaat accatctttt aatttaaccc tggcggagac gccattattt ttggacgttc
     1021 ttgaaacaaa attaattcct gaagaatctt tctccagttt attcagctca agattcagtc
     1081 cat
//
LOCUS       DAZKHF020000566         1074 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2201S31241
            SAMN43267800-rid25106413.denovo.570, whole genome shotgun sequence.
ACCESSION   DAZKHF020000566 DAZKHF020000000
VERSION     DAZKHF020000566.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267800
            Sequence Read Archive: SRR30307263
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1074)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1074)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 1074)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350581.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350581.2
            Genome Coverage       :: 151x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:21:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,652
            CDSs (total)                      :: 5,557
            Genes (coding)                    :: 5,320
            CDSs (with protein)               :: 5,320
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 237
            CDSs (without protein)            :: 237
            Pseudo Genes (ambiguous residues) :: 0 of 237
            Pseudo Genes (frameshifted)       :: 102 of 237
            Pseudo Genes (incomplete)         :: 130 of 237
            Pseudo Genes (internal stop)      :: 52 of 237
            Pseudo Genes (multiple problems)  :: 41 of 237
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1074
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267800-rid25106413.denovo.570"
                     /serotype="O 26:H11"
                     /isolate="2201S31241"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-01"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            <1..375
                     /locus_tag="ACHOSC_RS25895"
                     /old_locus_tag="pgaptmp_005179"
     CDS             <1..375
                     /locus_tag="ACHOSC_RS25895"
                     /old_locus_tag="pgaptmp_005179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003796 - lysozyme activity [Evidence
                     IEA];
                     GO_process: GO:0009253 - peptidoglycan catabolic process
                     [Evidence IEA];
                     GO_process: GO:0016998 - cell wall macromolecule catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family protein"
                     /protein_id="HGU4550911.1"
                     /translation="GATRVDGKPVIPGMKLSKGKCDQVNAIERDKALAWVEKNIKVPL
                     TEPQKAGIASFCPYNIGPGKCFPSTFYKRINAGDRKGACEAIRWWIKDGGRDCRIRSN
                     NCYGQVSRRDQESALACWGIDR"
     gene            535..672
                     /locus_tag="ACHOSC_RS25900"
                     /old_locus_tag="pgaptmp_005180"
     CDS             535..672
                     /locus_tag="ACHOSC_RS25900"
                     /old_locus_tag="pgaptmp_005180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000459350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4550912.1"
                     /translation="MFDFSKLIREIRVMAEKLSTWKYILIWLVFVIMASGYFIGQIRW
                     W"
     gene            674..1012
                     /locus_tag="ACHOSC_RS25905"
                     /old_locus_tag="pgaptmp_005181"
                     /pseudo
     CDS             674..1012
                     /locus_tag="ACHOSC_RS25905"
                     /old_locus_tag="pgaptmp_005181"
                     /inference="COORDINATES: protein motif:HMM:NF015218.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="lysis protein"
     gene            984..>1074
                     /locus_tag="ACHOSC_RS25910"
                     /old_locus_tag="pgaptmp_005182"
     CDS             984..>1074
                     /locus_tag="ACHOSC_RS25910"
                     /old_locus_tag="pgaptmp_005182"
                     /inference="COORDINATES: protein motif:HMM:NF017860.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rz1 family lipoprotein"
                     /protein_id="HGU4550913.1"
                     /translation="MKPPAPPAWIMQPAPDWQTPLNGIISSSEN"
BASE COUNT          279 a          244 c          308 g          243 t
ORIGIN      
        1 ggagccaccc gggtggatgg taagcctgtt attcctggca tgaagctgtc gaaggggaaa
       61 tgcgaccagg ttaacgccat tgagcgtgat aaggcgctgg catgggtgga gaaaaacatc
      121 aaagtgccac tgactgaacc ccagaaagcg ggtattgcgt cattctgtcc ttacaacatt
      181 ggcccaggta agtgtttccc gtcgacgttt tataaacgaa ttaatgcagg agatcgaaaa
      241 ggtgcctgcg aagcgattcg ctggtggatt aaggacggtg gcagagactg ccgtattcgc
      301 tcaaataact gttatggtca ggtctcacgg cgtgaccagg agagcgcgct ggcgtgctgg
      361 ggaattgaca gataagcaga atattttgct gaaaaatgcg gtttgctcac acggacggat
      421 aacacaaaat cctgcgaact gacaaaaact aagtgaataa aagtaaaaac cccatttgtt
      481 ggctgcaagc ggggttttgt gtttcctgac tccggaaaag tcaaaggaga aagtgtgttt
      541 gattttagca aactgattcg ggagattcga gtgatggctg aaaaattatc aacctggaag
      601 tacatcctta tctggctggt gtttgtgatt atggcctccg gttatttcat cggtcagata
      661 cgctggtggt gaaatgaacc gcgtactgtg cgtggtcatc attgccctgc tggtggcctg
      721 tggtgcgctt agtctggggc tgaatcatta ccgcgataac gccatcacct acaaagcgca
      781 gcgcgataaa aaagccagag agctggagct ggcaaacgca actattactg atatgcaggt
      841 gcgccagcgc gatgttgctg cgctcgatgc aaaatactcg agggaattag ccgatgcgag
      901 agctgaaaat gaaactctgc gcgctgatgt tgccgctggt cgtaagcgcc tgcggatcaa
      961 cgccacctgc cccggtaccg tgcgtgaagc caccggcacc tccggcgtgg ataatgcaac
     1021 cggcccccga ctggcagaca ccgctgaacg ggattatttc atcctcagag aacg
//
LOCUS       DAZKHF020000567         1074 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2201S31241
            SAMN43267800-rid25106413.denovo.571, whole genome shotgun sequence.
ACCESSION   DAZKHF020000567 DAZKHF020000000
VERSION     DAZKHF020000567.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267800
            Sequence Read Archive: SRR30307263
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1074)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1074)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 1074)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350581.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350581.2
            Genome Coverage       :: 151x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:21:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,652
            CDSs (total)                      :: 5,557
            Genes (coding)                    :: 5,320
            CDSs (with protein)               :: 5,320
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 237
            CDSs (without protein)            :: 237
            Pseudo Genes (ambiguous residues) :: 0 of 237
            Pseudo Genes (frameshifted)       :: 102 of 237
            Pseudo Genes (incomplete)         :: 130 of 237
            Pseudo Genes (internal stop)      :: 52 of 237
            Pseudo Genes (multiple problems)  :: 41 of 237
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1074
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267800-rid25106413.denovo.571"
                     /serotype="O 26:H11"
                     /isolate="2201S31241"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-01"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            25..>1074
                     /locus_tag="ACHOSC_RS25915"
                     /old_locus_tag="pgaptmp_005183"
     CDS             25..>1074
                     /locus_tag="ACHOSC_RS25915"
                     /old_locus_tag="pgaptmp_005183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000533644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HGU4550914.1"
                     /translation="MGRRRSHERRDLPPNLYIRNNGYYCYRDPRTGKEFGLGRDRRIA
                     ITEAIQANIELFSGHKHKPLTARINSDNSVTLHSWLDRYEKILASRGIKQKTLINYMS
                     KIKAIRRGLPDAPLEDITTKEIAAMLSGYIDEGKAASAKLIRSTLSDAFREAIAEGHI
                     TTNPVAATRAAKSEVRRSRLTADEYLKIYQAAESSPCWLRLAMELAVVTGQRVGDLCE
                     MKWSDIVDGYLYVEQSKTGVKIAIPTTLHVDALGISMKETLDKCKEILGGETIIASTH
                     REPLSSGTVSRYFMRARKASGLSFEGDPPTFHELRSLSARLYEKQISDKFAQHLLGHK
                     SDTMASQYRDDRGREW"
BASE COUNT          346 a          226 c          265 g          237 t
ORIGIN      
        1 gtagcctttt gaagaggatc agaaatggga agaaggcgaa gtcatgagcg ccgggattta
       61 ccccctaatc tttatataag aaacaatgga tattactgct acagggaccc aaggacgggt
      121 aaagagtttg gattaggccg agacaggagg atagcaatca ctgaagcaat acaggccaac
      181 attgagttat tttcaggaca caaacacaag cctctgacag cgagaatcaa cagtgataat
      241 tctgttacgt tacattcatg gcttgatcgc tacgaaaaaa tcctcgccag cagaggaatc
      301 aagcagaaga ctctcataaa ttacatgagc aaaattaaag caataaggag ggggctgcct
      361 gatgctccac ttgaagacat caccacaaaa gaaattgcgg caatgctcag tggatacata
      421 gacgagggca aggcggcgtc agccaagtta atcagatcaa cactgagcga tgcattccga
      481 gaggcaatag ctgaaggcca tataacaaca aacccggtcg ctgccactcg cgcagcaaaa
      541 tcagaggtaa ggagatcaag acttacggct gacgaatacc tgaaaattta tcaagcagca
      601 gaatcatcac catgttggct aagacttgca atggagctgg ctgttgttac cgggcagcga
      661 gttggtgatt tatgcgaaat gaagtggtct gatatcgtag atggatatct ttatgtcgag
      721 caaagcaaaa caggcgtaaa aattgccatc cctacaacat tgcatgttga tgctctcggg
      781 atatcaatga aggaaacact tgataaatgc aaagagattc ttggcggaga aaccataatt
      841 gcatctactc atcgtgaacc gctttcatcc ggcacagtat caaggtattt tatgcgcgca
      901 cgaaaagcat caggtctttc cttcgaaggg gatccgccga cctttcacga gttgcgcagt
      961 ttgtctgcaa gactctatga gaagcagata agcgataagt ttgctcaaca tcttctcggg
     1021 cataagtcgg acaccatggc atcacagtat cgtgatgaca gaggcaggga gtgg
//
LOCUS       DAZKHF020000568         1073 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2201S31241
            SAMN43267800-rid25106413.denovo.572, whole genome shotgun sequence.
ACCESSION   DAZKHF020000568 DAZKHF020000000
VERSION     DAZKHF020000568.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267800
            Sequence Read Archive: SRR30307263
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1073)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1073)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 1073)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350581.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350581.2
            Genome Coverage       :: 151x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:21:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,652
            CDSs (total)                      :: 5,557
            Genes (coding)                    :: 5,320
            CDSs (with protein)               :: 5,320
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 237
            CDSs (without protein)            :: 237
            Pseudo Genes (ambiguous residues) :: 0 of 237
            Pseudo Genes (frameshifted)       :: 102 of 237
            Pseudo Genes (incomplete)         :: 130 of 237
            Pseudo Genes (internal stop)      :: 52 of 237
            Pseudo Genes (multiple problems)  :: 41 of 237
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1073
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267800-rid25106413.denovo.572"
                     /serotype="O 26:H11"
                     /isolate="2201S31241"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-01"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            <1..945
                     /locus_tag="ACHOSC_RS25920"
                     /old_locus_tag="pgaptmp_005184"
     CDS             <1..945
                     /locus_tag="ACHOSC_RS25920"
                     /old_locus_tag="pgaptmp_005184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001405821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3987 domain-containing protein"
                     /protein_id="HGU4550915.1"
                     /translation="GIMSDEAGIIFDGYTLSELPFINKMWDGSVLSVDRKNELEQMIE
                     NARMTLSLMVQPGLFDRYMERKGSVARDSGFLARCLISKPATTQGKRFINGAVIPGGS
                     LTAFHERLMELARGSIEKSSEDERYCLHFSPEAQKIFIEHYNVLEQDLSPSGPLSPFR
                     GHVSKKTENIARIAALFQYFSYGEGKISADIMTSAVVISSWYTDEYKKLFALPDESEL
                     QQKDAEELFDWLIEECRGECPPRVRKNYILQCGPGRFRNRKKLNALLNILESQFRLSV
                     VPEGKTMYVLLPQIASLKLSDVSGIFTSGYHYNKLRAK"
BASE COUNT          298 a          203 c          264 g          308 t
ORIGIN      
        1 ggtattatgt cagatgaagc cggtattata tttgatggat ataccctgtc tgagttgcct
       61 tttattaaca aaatgtggga tggctctgta ttgtctgtgg acagaaagaa tgaactcgaa
      121 cagatgattg aaaatgccag aatgacgtta tccctcatgg tgcaacccgg tctttttgac
      181 cgatatatgg agcgaaaagg ttctgttgcc agggacagtg gttttcttgc ccgttgtctt
      241 atcagtaaac ctgcaaccac gcaggggaaa cgctttatta acggagctgt gattccggga
      301 ggatcattaa cggcatttca tgaacgatta atggaactgg ccagaggcag tattgaaaaa
      361 tccagtgagg atgaacggta ttgtctgcat ttttcgcctg aagcgcaaaa aatatttatt
      421 gagcactata atgttctgga gcaggattta tccccttccg gacccttaag cccgtttcgg
      481 ggacatgttt caaaaaaaac agaaaacatt gcaagaattg cagcgttatt tcagtatttc
      541 agctatggtg aagggaaaat ctctgccgat atcatgacgt ctgctgtcgt aataagttcg
      601 tggtacacag atgaatataa aaaattattt gcgttacctg atgaatctga actgcagcag
      661 aaggacgccg aggagctgtt cgactggctc attgaggagt gccgcggaga atgcccgccc
      721 cgtgtcagga aaaattatat tctccagtgt ggcccgggcc ggttcagaaa caggaaaaaa
      781 ctgaacgcat tgctgaacat tcttgaatct cagttcaggc tttctgtcgt gccggaagga
      841 aaaaccatgt atgttctgct tccccagata gccagtttaa agttgtctga tgtctctggt
      901 atttttacct cgggttatca ctacaataag ctgagagcga agtaaccggt gagcagtttt
      961 tctccattat gcttcagact tagatgattg attattatgg ggatttcatc accgatattc
     1021 agatgtatga atatcggtga tgaactaaag ctcaggagtt gcgtggtggt gat
//
LOCUS       DAZKHF020000569         1071 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2201S31241
            SAMN43267800-rid25106413.denovo.573, whole genome shotgun sequence.
ACCESSION   DAZKHF020000569 DAZKHF020000000
VERSION     DAZKHF020000569.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267800
            Sequence Read Archive: SRR30307263
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1071)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1071)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 1071)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350581.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350581.2
            Genome Coverage       :: 151x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:21:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,652
            CDSs (total)                      :: 5,557
            Genes (coding)                    :: 5,320
            CDSs (with protein)               :: 5,320
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 237
            CDSs (without protein)            :: 237
            Pseudo Genes (ambiguous residues) :: 0 of 237
            Pseudo Genes (frameshifted)       :: 102 of 237
            Pseudo Genes (incomplete)         :: 130 of 237
            Pseudo Genes (internal stop)      :: 52 of 237
            Pseudo Genes (multiple problems)  :: 41 of 237
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1071
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267800-rid25106413.denovo.573"
                     /serotype="O 26:H11"
                     /isolate="2201S31241"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-01"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            <1..1042
                     /locus_tag="ACHOSC_RS25925"
                     /old_locus_tag="pgaptmp_005185"
     CDS             <1..1042
                     /locus_tag="ACHOSC_RS25925"
                     /old_locus_tag="pgaptmp_005185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000606968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004197 - cysteine-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="EspG domain-containing protein"
                     /protein_id="HGU4550916.1"
                     /translation="KLLKVLSFGLWNPTYTRSERQTFQELLTVLEPVSPAPNELGRIY
                     ANFADGSSLRISVTNSELVEAEIRTPDNEKILMLLESNEQNRLLQSLPINLHMPYIQV
                     HRALSKMDLTDHKSMHNLLSFTSKLSATLIPHNTQTDPLSGPTPFSSMFMDTFRGLGN
                     AKLSLNGVDIPVDAQKLLRDALGLKDTHSSLARNVINNGISRHHAKQIARESSGSDKQ
                     KAEVVEFLCHPEAATAICSAFYQSFNVPALMLTHTRISQAREYNVERSLDVPNACINI
                     SISQSPDGSIHVASHTGILIMAPEDRPNELGMLTNRTSYEVPQGVKCEIDEMVRTLQP
                     RYGASETYLKNI"
BASE COUNT          350 a          237 c          204 g          280 t
ORIGIN      
        1 aaaattacta aaagttctta gttttggttt atggaatcca acgtatactc gtagtgaaag
       61 gcaaacattt caagagttgt taaccgtttt agaacctgta tctcccgcgc ctaatgaatt
      121 gggcagaata tatgctaatt ttgcagatgg ctcatcctta agaatttccg tcactaacag
      181 cgaacttgtt gaagccgaga ttcgcacacc agataatgaa aagattctta tgctcctgga
      241 gtcaaacgaa caaaataggt tattacaatc tttacccatc aatctccaca tgccatacat
      301 tcaggttcat cgtgccttat ctaagatgga cctgactgat cataagtcaa tgcacaatct
      361 acttagtttt acgtcaaaac tatcagcaac cttgattcct cataatactc aaacagatcc
      421 gctttccggg cctacaccat tcagctctat gtttatggat acatttcgag gactcggcaa
      481 tgcaaagctt tcactcaatg gtgttgatat acctgtagat gcacaaaaat tgcttcgcga
      541 tgcactagga cttaaagaca cacattcatc acttgcccgg aatgttataa ataatggtat
      601 ttctcgtcat catgcaaagc aaatagcaag ggaaagcagc ggcagcgata aacagaaagc
      661 tgaagttgtg gagtttttat gccaccccga agcggcaaca gccatatgct cggctttcta
      721 tcaatctttc aatgtgccag ccttaatgtt gacacataca aggatctctc aagccagaga
      781 atacaatgtg gaaagatcgt tagatgtacc taacgcgtgc attaacataa gtatctctca
      841 atcaccagat ggaagcattc acgttgccag ccataccggg attctgataa tggcgccaga
      901 agaccgcccc aacgagttag gaatgttgac gaacaggacc tcctatgaag tgccacaagg
      961 tgtgaaatgt gaaatcgatg agatggtaag aacgctacaa ccaaggtatg gcgcatctga
     1021 aacatacctg aaaaacattt aaaaataaaa aaatggacac cacacacttt a
//
LOCUS       DAZKHF020000570         1069 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2201S31241
            SAMN43267800-rid25106413.denovo.574, whole genome shotgun sequence.
ACCESSION   DAZKHF020000570 DAZKHF020000000
VERSION     DAZKHF020000570.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267800
            Sequence Read Archive: SRR30307263
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1069)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1069)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 1069)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350581.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350581.2
            Genome Coverage       :: 151x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:21:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,652
            CDSs (total)                      :: 5,557
            Genes (coding)                    :: 5,320
            CDSs (with protein)               :: 5,320
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 237
            CDSs (without protein)            :: 237
            Pseudo Genes (ambiguous residues) :: 0 of 237
            Pseudo Genes (frameshifted)       :: 102 of 237
            Pseudo Genes (incomplete)         :: 130 of 237
            Pseudo Genes (internal stop)      :: 52 of 237
            Pseudo Genes (multiple problems)  :: 41 of 237
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1069
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267800-rid25106413.denovo.574"
                     /serotype="O 26:H11"
                     /isolate="2201S31241"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-01"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            complement(1..1069)
                     /locus_tag="ACHOSC_RS25930"
                     /old_locus_tag="pgaptmp_005186"
                     /pseudo
     CDS             complement(1..1069)
                     /locus_tag="ACHOSC_RS25930"
                     /old_locus_tag="pgaptmp_005186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309822.1"
                     /note="frameshifted; incomplete; missing N-terminus and
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
BASE COUNT          215 a          343 c          252 g          259 t
ORIGIN      
        1 accatcgcct gcagaaccgg acgcacctca tccaccgtca catgcttctg taataccgcc
       61 gacagggaag ccagtttctc ttcaaacgct tgtgcctgcg cggccatctt cccctcaaat
      121 gtgcactgta aatccgccag caccgtggag aattcttcgc ccagtgcacg aataatggac
      181 agttcccgtt ccgtcatttt ctcagtatcc ccctgaacat cgctttcact gcatcatgct
      241 ctgtttcact gattgcctta ttaccgtcag atgcgccgtc aggcagctgt gatgaaactg
      301 ttttcccggc tgacgcgaac ggatcctcac gggcatcacg acgggacagc gcgtccagac
      361 tgtagttctg ctgctgaaga tacagtgcat caccgccggc aaggggcggc aggttctcac
      421 gtttacgggc ctcattgggc gtgagaagcg tatttttcac cgattcaccc agcgttttca
      481 tgcgccgttc gctgtccatt ctcagcagcg tggtgacgtc aaactccgtg ctctcgtttt
      541 cccctgtttc cagcgcctca tccagtaaca gctcaatgga ctcaatcagc gtctgcagac
      601 actgggaata atactgctgc tccagcgcct ccacgttgtc actggaaggc ggctggccaa
      661 cgccaatctt gtaggccggg acacggaaca ccgaacagac aatttcagcc gtcatcttca
      721 gttgttccac cgtctgcgca tccaccggtg aaaacgtcgt ggggttatat ttcgccccgt
      781 tgctcaggat cgccgttttc cccgcatttt cgccggtata cccgctgtcc cagttgctct
      841 tcagtttttt cgcattttct tccgttatac tgccggggat ctcaatcacc ccggacggcc
      901 tgccgccatt tctgaaaaaa gacgttgaat ttgcctgaat atgatgcccc tgcgtggccg
      961 ccagcccggc agcatacacc ggcggcagcc ccacaagcgg atgaaaaaaa cagttaaacc
     1021 ggtcgtggat cacttccctg gcaggcaccg tcaccgcctc cgtgatccc
//
LOCUS       DAZKHF020000571         1061 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2201S31241
            SAMN43267800-rid25106413.denovo.575, whole genome shotgun sequence.
ACCESSION   DAZKHF020000571 DAZKHF020000000
VERSION     DAZKHF020000571.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267800
            Sequence Read Archive: SRR30307263
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1061)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1061)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 1061)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350581.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350581.2
            Genome Coverage       :: 151x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:21:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,652
            CDSs (total)                      :: 5,557
            Genes (coding)                    :: 5,320
            CDSs (with protein)               :: 5,320
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 237
            CDSs (without protein)            :: 237
            Pseudo Genes (ambiguous residues) :: 0 of 237
            Pseudo Genes (frameshifted)       :: 102 of 237
            Pseudo Genes (incomplete)         :: 130 of 237
            Pseudo Genes (internal stop)      :: 52 of 237
            Pseudo Genes (multiple problems)  :: 41 of 237
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1061
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267800-rid25106413.denovo.575"
                     /serotype="O 26:H11"
                     /isolate="2201S31241"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-01"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            753..>1061
                     /locus_tag="ACHOSC_RS25935"
                     /old_locus_tag="pgaptmp_005187"
     CDS             753..>1061
                     /locus_tag="ACHOSC_RS25935"
                     /old_locus_tag="pgaptmp_005187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000594912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbiJ-NTD4 domain-containing protein"
                     /protein_id="HGU4550917.1"
                     /translation="MIKPFSVRYGHVDVREHVQLNDLNSDTRMALWNCLYLFLWTNNR
                     QTATATKCAQSVWIYYLNQPADNIPRYESGYKSDKTLLTAIRDYIYGEAWYLVYDLIE
                     F"
BASE COUNT          307 a          206 c          230 g          318 t
ORIGIN      
        1 aagtattttt tgtagagtca agtcccggtt tggatccgtt taacggaacc taactagatt
       61 gactatttct ctgatgattt ttggataaat aatcaacaaa aatcaaaatg tgaacagaga
      121 atggtggggg cacaaaaagg ggcataattg aattaattat aattaaattt caatttttac
      181 aataggttaa atgtgtgtgc gagtccggcc ttcgcaccaa gttactaaat tccttacaat
      241 acagttagtt agacgattaa atctgtctct tttgcctgta agaaatgctc tgttatgtcc
      301 cttgaaaaag ccgctgtggt tgatagtggt aattgggatg ttaactcata gcggcgtgtc
      361 gttccaagcg tcaaacacgt actgactcaa cttgaaagct atgacgtagc caccgtctct
      421 aaacaatccc aaaactttta gtgcagcgca ttttagcccg atgcccccat tctgtcgccg
      481 tcgcgagacg ccgcagggga taaacaggtt tatggtggtc acggtacttg cagaccatat
      541 gcaagctctg ccagcggttc ggcctcgctc cccttgggta catggtcatc ctgataagcc
      601 tcactaactg acataaccct gatgaagtcg caagaggctc cgcttgttgt cttaccacca
      661 acggggcttt atggattcta ttgcttaata tatgattgta tcaaacttag actgcataat
      721 ttaaacgaac ataacaatca ggcaggggaa gaatgataaa gccattttct gtgaggtatg
      781 gtcatgttga cgttagagag catgtccagc tgaatgatct taattctgat acacgcatgg
      841 cattgtggaa ttgcttatat cttttccttt ggactaacaa tcgtcaaact gctacagcaa
      901 caaaatgtgc gcagtctgtg tggatatatt atctaaatca acctgcggat aatattcctc
      961 gttatgaaag tggatacaaa agtgataaga cgttgctgac tgctatccga gattatatat
     1021 atggggaagc atggtatttg gtatatgatt taattgagtt c
//
LOCUS       DAZKHF020000572         1060 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2201S31241
            SAMN43267800-rid25106413.denovo.576, whole genome shotgun sequence.
ACCESSION   DAZKHF020000572 DAZKHF020000000
VERSION     DAZKHF020000572.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267800
            Sequence Read Archive: SRR30307263
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1060)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1060)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 1060)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350581.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350581.2
            Genome Coverage       :: 151x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:21:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,652
            CDSs (total)                      :: 5,557
            Genes (coding)                    :: 5,320
            CDSs (with protein)               :: 5,320
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 237
            CDSs (without protein)            :: 237
            Pseudo Genes (ambiguous residues) :: 0 of 237
            Pseudo Genes (frameshifted)       :: 102 of 237
            Pseudo Genes (incomplete)         :: 130 of 237
            Pseudo Genes (internal stop)      :: 52 of 237
            Pseudo Genes (multiple problems)  :: 41 of 237
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1060
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267800-rid25106413.denovo.576"
                     /serotype="O 26:H11"
                     /isolate="2201S31241"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-01"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            complement(331..>1060)
                     /locus_tag="ACHOSC_RS25940"
                     /old_locus_tag="pgaptmp_005188"
     CDS             complement(331..>1060)
                     /locus_tag="ACHOSC_RS25940"
                     /old_locus_tag="pgaptmp_005188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF4765 family protein"
                     /protein_id="HGU4550918.1"
                     /translation="KIVYKGESVDLTKEPNSELYSGRYSINNKQVNVYFFRDADGTFY
                     KSEGLHGGGVIRYIDKPYSQLREGDIGYDEDLLDIYDDSPVLEDTLPALSSEIVPTPE
                     HSIKQIYSKIKEGHIELSDSDIILCRGTTGIQAENIVEYKTAGGLPDSNPNVKAPDEY
                     MAQQQVRIGRILPEYTSDLSVADRFSREHYLIVVKVKAKYITRGSVTESGWVIDKTAP
                     VEPLAIIDRTFGMKENISMVNASK"
BASE COUNT          309 a          233 c          186 g          332 t
ORIGIN      
        1 gagtatttca tcagggcagg taaaaacaga gtaaatcagc aaaagaagct gatcttcagc
       61 gatactgaca ctaactgacg gtttaagcgg tcgtatgaag cagcagcttt ccgacggact
      121 gccatgcgga tcgtttacct tttgggctat tccgcccgtc atcaagcggc tcacgagtac
      181 tgagtttatc aggatgatat tgctgacaat ggtaattcgt tgaccgatgt gtacttcact
      241 atatatcggt caacaccgta ctgccgtgca acatctcaaa gatgtagcat cgtttttacc
      301 agaggaggca ggacatagat tgtaaaaaaa ctatttcgat gcatttacca ttgagatatt
      361 ttccttcata ccaaaagttc tatcaattat cgcaagtggt tcaacaggtg cggtcttatc
      421 tataacccaa ccactctctg taacacttcc tcgtgtgata tattttgcct ttactttaac
      481 aactattaga taatgctcac gactaaaccg atcagcaacg ctaagatccg atgtgtattc
      541 aggcaatatt cttccaatac gtacctgctg ttgtgccata tattcatctg gtgcttttac
      601 atttggattt gaatcaggaa gccctccagc agttttatat tcaacgatat tttcagcttg
      661 aataccggtt gtgccgcgac acaatatgat gtctgaatcg gacagttcta tgtgcccctc
      721 cttaattttc gaataaattt gtttaatact atgttctgga gttggtacta tttcagaaga
      781 taaagcaggc aacgtgtctt caagcaccgg agaatcatcg tatatatcca acaaatcctc
      841 atcataccca atatctcctt ctcttaactg agaatacggt ttatctatgt atctaataac
      901 tcccccacca tgaagacctt ctgatttata aaatgtacca tcagcgtcac gaaagaaata
      961 aacattaacc tgtttgttat ttatagaata cctacctgaa tataattccg aatttggctc
     1021 cttagttaaa tctactgatt cccccttgta gactatttta
//
LOCUS       DAZKHF020000573         1059 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2201S31241
            SAMN43267800-rid25106413.denovo.577, whole genome shotgun sequence.
ACCESSION   DAZKHF020000573 DAZKHF020000000
VERSION     DAZKHF020000573.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267800
            Sequence Read Archive: SRR30307263
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1059)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1059)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 1059)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350581.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350581.2
            Genome Coverage       :: 151x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:21:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,652
            CDSs (total)                      :: 5,557
            Genes (coding)                    :: 5,320
            CDSs (with protein)               :: 5,320
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 237
            CDSs (without protein)            :: 237
            Pseudo Genes (ambiguous residues) :: 0 of 237
            Pseudo Genes (frameshifted)       :: 102 of 237
            Pseudo Genes (incomplete)         :: 130 of 237
            Pseudo Genes (internal stop)      :: 52 of 237
            Pseudo Genes (multiple problems)  :: 41 of 237
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1059
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267800-rid25106413.denovo.577"
                     /serotype="O 26:H11"
                     /isolate="2201S31241"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-01"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            complement(510..1058)
                     /gene="hdeD"
                     /locus_tag="ACHOSC_RS25945"
                     /old_locus_tag="pgaptmp_005189"
     CDS             complement(510..1058)
                     /gene="hdeD"
                     /locus_tag="ACHOSC_RS25945"
                     /old_locus_tag="pgaptmp_005189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013368005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid-resistance protein HdeD"
                     /protein_id="HGU4550919.1"
                     /translation="MLKFDLEMLKKHRRAIQFIAVLLFIVGLLCISFPFVSGDILSTV
                     VGALLICSGIALIVGLFSNRSHNFWPVLSGFLVAVAYLLIGYFFIRAPELGIFAIAAF
                     IAGLFCVAGVIRLMSWYRQRSMKGSWLQLVIGVLDIVIAWIFLGATPMVSVTLVSTLV
                     GIELIFSAASLFSFASLFVKQQ"
BASE COUNT          356 a          238 c          212 g          253 t
ORIGIN      
        1 agaatattac taaagttaaa atgtaatccg atttaaatat cgagtctcct tgtttcgact
       61 taagctggca attggattgc cagctttctt ttaacgcatt gaaattacct gagtaaataa
      121 cgcattcatg tcttcaggtt tttatatagc gtctggagac acggatattt atgcaatgta
      181 atcaacgcca gttgcaaaaa aatgaacccg gattacttat ttaaccgccc atagcatcaa
      241 cgcttacgga actctttgtt cactttccca cagcactttt aaaacattaa acctgactac
      301 ggaaaatatc agccatgata atgtttgaaa tggctaattt gccatggagt gaaaaaaatt
      361 agatgaaatt cagtaggttg aaatgatcac tagcaggtaa ttatttcaat gatagtgcgc
      421 aattgatcta caacactgcg tagcggagag agtattaatc ggatcatagt cacatcaagt
      481 gactatgatc ctggtgacaa ccgggataat tattgctgct taacgaacaa actggcgaag
      541 ctgaacaggc tggcggcgct aaatatcagt tcaattccca ccagtgtgga aaccagcgtt
      601 acagacacca tcggcgttgc accaaggaat atccaggcaa tgacgatatc cagcacacca
      661 ataacgagct gtagccagct gcctttcatt gaacgctgac gataccaact catcaggcga
      721 ataacccctg caacacagaa caaaccggca ataaatgccg caatggcaaa aatgcccagc
      781 tccggtgcgc ggatgaagaa atagccgatc aataaatagg cgactgcgac gaggaaaccg
      841 gataataccg gccagaaatt atgactgcgg ttgctgaata acccgacaat aagcgcaata
      901 cccgagcaga ttaataatgc acccactact gtgcttaaaa tatcgccaga gacgaacggg
      961 aaactgatac acagcaaccc gacgataaac agcagcacgg caataaactg gattgctctg
     1021 cgatgctttt tcagcatctc cagatcaaac ttcaaaatt
//
LOCUS       DAZKHF020000574         1058 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2201S31241
            SAMN43267800-rid25106413.denovo.578, whole genome shotgun sequence.
ACCESSION   DAZKHF020000574 DAZKHF020000000
VERSION     DAZKHF020000574.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267800
            Sequence Read Archive: SRR30307263
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1058)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1058)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 1058)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350581.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350581.2
            Genome Coverage       :: 151x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:21:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,652
            CDSs (total)                      :: 5,557
            Genes (coding)                    :: 5,320
            CDSs (with protein)               :: 5,320
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 237
            CDSs (without protein)            :: 237
            Pseudo Genes (ambiguous residues) :: 0 of 237
            Pseudo Genes (frameshifted)       :: 102 of 237
            Pseudo Genes (incomplete)         :: 130 of 237
            Pseudo Genes (internal stop)      :: 52 of 237
            Pseudo Genes (multiple problems)  :: 41 of 237
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1058
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267800-rid25106413.denovo.578"
                     /serotype="O 26:H11"
                     /isolate="2201S31241"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-01"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            complement(<1..126)
                     /locus_tag="ACHOSC_RS25950"
                     /old_locus_tag="pgaptmp_005190"
     CDS             complement(<1..126)
                     /locus_tag="ACHOSC_RS25950"
                     /old_locus_tag="pgaptmp_005190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415090.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1398 family protein"
                     /protein_id="HGU4550920.1"
                     /translation="MAQAAIFKEIFDQVRKDLNCELFYSELKRHNVSLYIYYLATD"
     gene            427..633
                     /locus_tag="ACHOSC_RS25955"
                     /old_locus_tag="pgaptmp_005191"
     CDS             427..633
                     /locus_tag="ACHOSC_RS25955"
                     /old_locus_tag="pgaptmp_005191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4550921.1"
                     /translation="MNKEQSADEVSLDLIRVKNMLNSTISMSYPDVVIACIEHQVSLE
                     AFRAIEAALVKHDKNSKDYSLVVD"
BASE COUNT          284 a          203 c          243 g          328 t
ORIGIN      
        1 atctgtggct aaatagtaaa tataaagtga gacattgtga cgttttagct cagaataaaa
       61 taattcacag tttaaatctt tacgcacttg atcgaatatt tctttaaaaa tggcagcctg
      121 agccattggt aaaccttcca tgtgatacga tggcgcgtag ttagcattat cgtgtttatt
      181 gtttcaatct ggtctgacct ctttgtgttt tgttgatgat ttatgtcaaa tattaagcct
      241 gtttttaatg aatagtgttg attgcgtaac aaaattgagc cttgctggca tccaggaggg
      301 atatgcaacc gacagatgta tgtaaggtcg atgtactcaa actttcatac ttttcctctt
      361 ttatgcagta agatttgaag taatatttta accgctagat gacgagcaaa cgcatggagc
      421 gacaaaatga ataaagaaca atctgctgat gaagtctcgt tggatctgat tcgtgtaaaa
      481 aatatgctta atagcaccat ttctatgagt tacccggatg ttgtaattgc atgtatagaa
      541 catcaggtgt ctctggaagc attcagggca attgaggcag cgttggtgaa gcacgataag
      601 aattcgaagg attattccct ggtggttgac tgagcaccat aactgctaat cattcaaact
      661 atttcacctg tgacagagtc aatatcgcat tctgtcactg tcaggctaat acagagctgc
      721 aattcaacta ctgcaatgtc ctcgtaatta ggtgaattta caatatcgtc ctgttcggat
      781 gccggttgca ttgctgaaga tgaggcattt atggttcgca tattttcccc tcatgctcgt
      841 cagtcctgtg cgtaggaaga aacaggacac tcacactaat ttgtgtgggc atgctgtgat
      901 gtccttctga attattccta tgccattatg taaagcgctg tatcagatgc tcatcacggc
      961 tgtcaggctg tcaggctgtc gggtcctccc ggtgggggcc cctgccacgg ggcgggagcg
     1021 tcgcggaaaa aggctagttt ttgcatttcc atggcggc
//
LOCUS       DAZKHF020000575         1057 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2201S31241
            SAMN43267800-rid25106413.denovo.579, whole genome shotgun sequence.
ACCESSION   DAZKHF020000575 DAZKHF020000000
VERSION     DAZKHF020000575.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267800
            Sequence Read Archive: SRR30307263
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1057)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1057)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 1057)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350581.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350581.2
            Genome Coverage       :: 151x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:21:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,652
            CDSs (total)                      :: 5,557
            Genes (coding)                    :: 5,320
            CDSs (with protein)               :: 5,320
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 237
            CDSs (without protein)            :: 237
            Pseudo Genes (ambiguous residues) :: 0 of 237
            Pseudo Genes (frameshifted)       :: 102 of 237
            Pseudo Genes (incomplete)         :: 130 of 237
            Pseudo Genes (internal stop)      :: 52 of 237
            Pseudo Genes (multiple problems)  :: 41 of 237
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1057
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267800-rid25106413.denovo.579"
                     /serotype="O 26:H11"
                     /isolate="2201S31241"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-01"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            complement(<1..223)
                     /locus_tag="ACHOSC_RS25960"
                     /old_locus_tag="pgaptmp_005192"
     CDS             complement(<1..223)
                     /locus_tag="ACHOSC_RS25960"
                     /old_locus_tag="pgaptmp_005192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016243610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4550922.1"
                     /translation="MHHIKTAADLNCFLNSMVAVSQPTVDKIIFGAEATLINKIGTCW
                     IASIDVLRKAVFEGANIIITHEPTFYSYAD"
     gene            complement(246..>1057)
                     /locus_tag="ACHOSC_RS25965"
                     /old_locus_tag="pgaptmp_005193"
     CDS             complement(246..>1057)
                     /locus_tag="ACHOSC_RS25965"
                     /old_locus_tag="pgaptmp_005193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001342239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HGU4550923.1"
                     /translation="YHVLQSFPMPQLASSREIRILLPASYYESDVSYPVLYMHDGQNL
                     FDKSVSFGPHVWDIPEAVDAFFPNSQYDGVIIVGIDNASSHGKFSRMDEYSPWPLNTS
                     FPLPGWDPDVDYSGGKGALYVDFIVNTLKNYIDSNFRTLPDRNNTAIAGSSMGAYISL
                     FAAILRLDVFSKVGVFSPALWFNDSAMLNFIQENNIFADLTVYLDVGTLETSGMREDF
                     PEVYISGAEKLCVSLRKQRNVTIDYHLWGGDTHSESAWAKRFPEMLKLFYC"
BASE COUNT          352 a          227 c          192 g          286 t
ORIGIN      
        1 gatcagcata agaataaaat gtaggctcat gagtaataat gatattggca ccttcaaaaa
       61 cagcctttct taaaacatca attgaggcaa tccagcaggt cccaatttta ttaataagtg
      121 tcgcctcagc accaaaaata attttatcaa cagttggttg cgaaacggca accattgaat
      181 ttaaaaaaca attaagatct gcggctgttt taatatgatg cattcaacat ccttcaccgg
      241 tttcatcagc aataaaataa tttcaacatt tctggaaaac gttttgccca tgcagactca
      301 gagtgggtat cacctcccca tagatgataa tctatagtta cattgcgttg tttacggaga
      361 ctcacgcata atttttccgc gcctgaaata tagacttcag gaaagtcttc gcgcatacca
      421 ctggtttcca gcgtaccgac atcgagatat acagtaagat ctgcaaagat attgttttcc
      481 tgaataaaat tcagcattgc gctgtcattg aaccatagtg ccggcgaaaa aacgccaact
      541 tttgaaaaaa cgtctagacg aagaattgcg gcaaacaaac tgatgtacgc acccatagaa
      601 ctacctgcta tcgctgtatt attacgatct gggagagttc gaaaattgct gtcaatatag
      661 ttcttgagcg tattcacaat aaaatcaaca tacaacgcac cttttcctcc actataatcc
      721 acatcagggt cccagccagg taagggaaaa gatgtgttta gcggccatgg agaatactca
      781 tccatacggc tgaatttacc atgagacgat gcattatcaa tccccacaat gataacaccg
      841 tcgtattggg aatttgggaa aaaagcatct accgcctcag gtatatccca tacatgtgga
      901 ccaaaagata cagatttatc aaataaattt tgcccatcgt gcatatacaa tacagggtaa
      961 ctgacatcag attcataata ggacgcaggt agtaatattc ttatttcgcg actacttgcc
     1021 aattgtggca tcggaaagga ttgcaacaca tgatatc
//
LOCUS       DAZKHF020000576         1055 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2201S31241
            SAMN43267800-rid25106413.denovo.580, whole genome shotgun sequence.
ACCESSION   DAZKHF020000576 DAZKHF020000000
VERSION     DAZKHF020000576.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267800
            Sequence Read Archive: SRR30307263
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1055)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1055)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 1055)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350581.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350581.2
            Genome Coverage       :: 151x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:21:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,652
            CDSs (total)                      :: 5,557
            Genes (coding)                    :: 5,320
            CDSs (with protein)               :: 5,320
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 237
            CDSs (without protein)            :: 237
            Pseudo Genes (ambiguous residues) :: 0 of 237
            Pseudo Genes (frameshifted)       :: 102 of 237
            Pseudo Genes (incomplete)         :: 130 of 237
            Pseudo Genes (internal stop)      :: 52 of 237
            Pseudo Genes (multiple problems)  :: 41 of 237
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1055
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267800-rid25106413.denovo.580"
                     /serotype="O 26:H11"
                     /isolate="2201S31241"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-01"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            <1..>1055
                     /locus_tag="ACHOSC_RS25970"
                     /old_locus_tag="pgaptmp_005194"
     CDS             <1..>1055
                     /locus_tag="ACHOSC_RS25970"
                     /old_locus_tag="pgaptmp_005194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HGU4550924.1"
                     /translation="KGGGRAHTPREAKDNLKSTQMMSVIDAIGEGPIEGPVKGLQSIL
                     VNKTPLTDTDGNPVIHGVTAVWRAGEQEQTPPEGFESSGSETALGVEVTKAKPVTRTI
                     TSANIDRLRVTFGVQSLVETTSKGDRNPASVRLLIQLQRNGNWVTEKDVTINGKTTSQ
                     FLASVILDNLPPRPFNIRMVRETADSTTDQLQNKTLWSSYTEIIDVKQCYPNTAIVGL
                     QVDAEQFGGQQMTVNYHIRGRIIQVPSNYDPEKRTYSGIWDGSLKPAYSNNPAWCLWD
                     MLTHPRYGMGKRLGAADVDKWALYAIAQYCDQTVPDGFGGTEPRMTFNAYLSQQRKAW
                     DVLSDFCSAMRCMPVWNG"
BASE COUNT          247 a          282 c          339 g          187 t
ORIGIN      
        1 aaaggtggcg gcagggcgca cacgcctcga gaggcgaagg ataatctcaa atccacgcag
       61 atgatgagtg tgattgatgc gattggtgag ggaccgatag aaggcccggt gaaggggctg
      121 cagagtatcc tggtgaacaa aaccccgctg acggacacgg acggcaatcc cgtgatacac
      181 ggtgtgaccg cggtctggcg cgccggggag caggagcaga caccgccgga aggttttgag
      241 tcatccggct ctgaaaccgc actgggcgtg gaagtgacga aggcaaagcc ggtgacgcgc
      301 accattacgt ccgcgaacat tgaccgcctg cgggttacct tcggggtgca gtcactggtg
      361 gagaccacct caaagggtga ccgtaacccg gcatccgtcc gcctgctgat tcagttacag
      421 cgtaacggta actgggtgac agaaaaggac gtcaccatta acggcaagac cacctcacag
      481 ttcctggcct cggtgattct ggataatctg cctccccggc cctttaacat ccggatggtc
      541 agggagacgg cggacagcac cacggaccag ctgcagaata agacgctgtg gtcgtcatac
      601 accgaaatca tcgatgtgaa acagtgctac ccgaacacgg ccattgtggg gctgcaggtg
      661 gatgcggagc agttcggcgg tcagcagatg acggtgaact accatatccg cggtcgcatc
      721 atccaggtgc cgtcaaacta tgacccggaa aaacgcacgt acagtggtat ctgggacggc
      781 agtctgaaac cggcatacag caacaacccg gcctggtgtc tgtgggacat gctgactcac
      841 ccgcgctacg gcatgggaaa acgtctgggg gcggcggatg tggacaaatg ggcgctgtat
      901 gccattgcgc agtactgcga ccagacggtg ccggatggtt tcgggggcac agagccgcgg
      961 atgaccttta atgcatacct gtcacaacag cgtaaggcgt gggacgtcct cagtgatttc
     1021 tgctcggcga tgcgctgtat gccggtatgg aacgg
//
LOCUS       DAZKHF020000577         1055 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2201S31241
            SAMN43267800-rid25106413.denovo.581, whole genome shotgun sequence.
ACCESSION   DAZKHF020000577 DAZKHF020000000
VERSION     DAZKHF020000577.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267800
            Sequence Read Archive: SRR30307263
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1055)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1055)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 1055)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350581.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350581.2
            Genome Coverage       :: 151x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:21:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,652
            CDSs (total)                      :: 5,557
            Genes (coding)                    :: 5,320
            CDSs (with protein)               :: 5,320
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 237
            CDSs (without protein)            :: 237
            Pseudo Genes (ambiguous residues) :: 0 of 237
            Pseudo Genes (frameshifted)       :: 102 of 237
            Pseudo Genes (incomplete)         :: 130 of 237
            Pseudo Genes (internal stop)      :: 52 of 237
            Pseudo Genes (multiple problems)  :: 41 of 237
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1055
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267800-rid25106413.denovo.581"
                     /serotype="O 26:H11"
                     /isolate="2201S31241"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-01"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            <1..>1055
                     /locus_tag="ACHOSC_RS25975"
                     /old_locus_tag="pgaptmp_005195"
     CDS             <1..>1055
                     /locus_tag="ACHOSC_RS25975"
                     /old_locus_tag="pgaptmp_005195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1983 domain-containing protein"
                     /protein_id="HGU4550925.1"
                     /translation="AHFDGDQSGTLNSVIPPAVQHLTVEVSAADGQYLAQAKWDTPRV
                     VKGVRFSLRLTSGSGEGSRLVTTAITADTEHRSSGLPLGEYTLTVRAINSYGQQGEPA
                     TTTFRINAPAKPATIELTPGYFQITAVPRLAVYDPTVQFEFWFSEAKIADTSQVETSA
                     RYLGTGSQWSVSGPHIKPGKDFWFYVRSVNLVGKSAFVEASGQASNDAEGYLDFFRGE
                     IGKTHLAQGLWELIDNSQLADEMAEMKTSITETRNEITQTVNKTLEDQSATIQQIQRV
                     QKDTNDDLAALYMLKVQKTKDGIPYVAGIGAGIEDTDGQPLSNILLLADRIAMINPEN
                     GNSTPLFVAQGNQLFMN"
BASE COUNT          267 a          272 c          328 g          188 t
ORIGIN      
        1 gcgcactttg acggcgacca gagcggcacc ctgaacagcg tcatccctcc ggcagtgcag
       61 cacctgacgg tggaggtgag cgcagctgac ggccagtatc tggcgcaggc gaaatgggac
      121 acgccgcggg tggtgaaggg ggtgcgcttc agtctgcgac tgaccagcgg aagcggagaa
      181 ggcagccgtc tggtgaccac cgccatcacc gcggatacag agcatcgttc cagtggtctg
      241 ccgctcgggg aatacaccct gacagtcagg gcaattaaca gttatggcca gcagggcgaa
      301 ccggccacca ccaccttccg gattaacgcg ccagcaaaac ccgccaccat tgaactgacg
      361 ccggggtatt ttcagataac ggcggtcccg cgtcttgcgg tgtatgaccc gacggtacag
      421 tttgagttct ggttttcgga ggcaaaaatc gcagacacat ctcaggtgga aacctctgcc
      481 cgttatctgg ggaccggcag tcagtggagt gtatccggcc cgcacattaa gcccgggaag
      541 gatttctggt tttacgtgcg cagcgtcaac ctggtgggga aatctgcgtt tgtggaagcc
      601 agcgggcagg ccagcaatga tgcagaaggg tatctggact ttttcagagg agaaatagga
      661 aaaacgcatc tggctcaggg gttgtgggaa ctgatagata acagccagct tgcagatgag
      721 atggcggaga tgaagaccag catcacggaa acccgcaatg aaatcacaca gacggtcaac
      781 aaaacgctgg aggaccagag cgccaccata cagcagatac agcgcgtgca gaaggacaca
      841 aatgatgacc tggctgcgct gtacatgctg aaggttcaaa aaacgaaaga cggcattccc
      901 tatgtggccg ggattggtgc agggattgag gatactgatg gccagccact gagcaacata
      961 ctgctgctgg ctgaccgtat cgcgatgata aatccggaga acggcaacag cacgccgtta
     1021 tttgtggcgc aggggaatca gctgttcatg aacga
//
LOCUS       DAZKHF020000578         1054 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2201S31241
            SAMN43267800-rid25106413.denovo.582, whole genome shotgun sequence.
ACCESSION   DAZKHF020000578 DAZKHF020000000
VERSION     DAZKHF020000578.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267800
            Sequence Read Archive: SRR30307263
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1054)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1054)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 1054)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350581.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350581.2
            Genome Coverage       :: 151x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:21:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,652
            CDSs (total)                      :: 5,557
            Genes (coding)                    :: 5,320
            CDSs (with protein)               :: 5,320
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 237
            CDSs (without protein)            :: 237
            Pseudo Genes (ambiguous residues) :: 0 of 237
            Pseudo Genes (frameshifted)       :: 102 of 237
            Pseudo Genes (incomplete)         :: 130 of 237
            Pseudo Genes (internal stop)      :: 52 of 237
            Pseudo Genes (multiple problems)  :: 41 of 237
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1054
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267800-rid25106413.denovo.582"
                     /serotype="O 26:H11"
                     /isolate="2201S31241"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-01"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            <1..197
                     /locus_tag="ACHOSC_RS25980"
                     /old_locus_tag="pgaptmp_005196"
     CDS             <1..197
                     /locus_tag="ACHOSC_RS25980"
                     /old_locus_tag="pgaptmp_005196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000898166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4550926.1"
                     /translation="KEQIIKYKDEMLKINPQITEMVAKYAGCSVEEVERCVEKYFSPS
                     SPSTPSLNELIKLKAKEITK"
     gene            194..556
                     /locus_tag="ACHOSC_RS25985"
                     /old_locus_tag="pgaptmp_005197"
     CDS             194..556
                     /locus_tag="ACHOSC_RS25985"
                     /old_locus_tag="pgaptmp_005197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000582632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4550927.1"
                     /translation="MIIDLDTLFPVRKTFTDIVSYCTDPFASVESKVFASLPADVECG
                     DLITSTGAKYVSGDDIYVVMSEFVTAGENKPVDVLRSNAGLVCIKADALNAVSEAAKT
                     ALIKKGFQLEGFHSVFTS"
     gene            575..>1054
                     /locus_tag="ACHOSC_RS25990"
                     /old_locus_tag="pgaptmp_005198"
     CDS             575..>1054
                     /locus_tag="ACHOSC_RS25990"
                     /old_locus_tag="pgaptmp_005198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001713133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="major capsid protein"
                     /protein_id="HGU4550928.1"
                     /translation="MILGNDYVDLIPLFQTHSTRNFLLSTLDFTDSVGVTSHKVAVSQ
                     LVESNESLFNKETSRFSSEHNVTKREQGKEWLIEIPYFLREDVIRPSDVQGKRKPGTD
                     FQETLTDIYAEYIAKHHVAYQRTKESVLAASLFSGKTYTPKTDDVLIEWGKLFNVSAM
                     "
BASE COUNT          326 a          199 c          212 g          317 t
ORIGIN      
        1 ataaagagca aatcatcaag tataaagatg aaatgctgaa aataaatcca caaattaccg
       61 aaatggtagc taaatacgct gggtgttctg tagaagaagt agaaagatgt gttgaaaaat
      121 atttttctcc ttcttctccg tctacacctt cccttaatga attaatcaaa ttaaaagcta
      181 aggagattac taaatgatta ttgatttaga cactctgttt ccagttcgaa agactttcac
      241 agatattgta agttattgta ctgatccgtt tgcctctgta gaaagcaagg tttttgcttc
      301 tcttcctgct gatgttgaat gcggcgactt gatcaccagc actggcgcta aatatgtatc
      361 aggagatgat atctatgttg ttatgagtga atttgtaacg gctggagaaa acaaacctgt
      421 agatgtttta cgtagcaatg ctgggcttgt atgcatcaaa gctgatgcgc tgaatgctgt
      481 aagtgaagca gcaaaaacag cattaattaa aaaaggcttc cagcttgaag ggttccattc
      541 tgttttcact tcttaataaa aggaataata ataaatgatt ctaggcaatg attacgttga
      601 tttgatccct ctgttccaga cacacagcac gcgtaacttc ctgctctcta ctcttgattt
      661 tacggattct gtaggggtta catctcataa agtagctgtt tcccagcttg tcgaaagcaa
      721 cgaatctctc tttaacaaag agacaagccg ttttagttct gaacacaatg ttactaagcg
      781 agaacaaggt aaagaatggc tgatcgagat cccttacttc ctgcgtgaag atgtgatccg
      841 tccttctgat gttcaaggca aacgtaaacc tggcactgat ttccaggaaa cactcactga
      901 tatttatgcg gaatatatcg ctaaacacca cgtagcgtat cagcgtacaa aagagagcgt
      961 attggctgct tctttgttct cgggcaaaac ttatacaccg aaaactgatg atgttcttat
     1021 cgaatggggc aagctgttta acgtatcagc tatg
//
LOCUS       DAZKHF020000579         1053 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2201S31241
            SAMN43267800-rid25106413.denovo.583, whole genome shotgun sequence.
ACCESSION   DAZKHF020000579 DAZKHF020000000
VERSION     DAZKHF020000579.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267800
            Sequence Read Archive: SRR30307263
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1053)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1053)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 1053)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350581.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350581.2
            Genome Coverage       :: 151x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:21:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,652
            CDSs (total)                      :: 5,557
            Genes (coding)                    :: 5,320
            CDSs (with protein)               :: 5,320
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 237
            CDSs (without protein)            :: 237
            Pseudo Genes (ambiguous residues) :: 0 of 237
            Pseudo Genes (frameshifted)       :: 102 of 237
            Pseudo Genes (incomplete)         :: 130 of 237
            Pseudo Genes (internal stop)      :: 52 of 237
            Pseudo Genes (multiple problems)  :: 41 of 237
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1053
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267800-rid25106413.denovo.583"
                     /serotype="O 26:H11"
                     /isolate="2201S31241"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-01"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            complement(<1..>1053)
                     /locus_tag="ACHOSC_RS25995"
                     /old_locus_tag="pgaptmp_005199"
     CDS             complement(<1..>1053)
                     /locus_tag="ACHOSC_RS25995"
                     /old_locus_tag="pgaptmp_005199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001536548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="AAA domain-containing protein"
                     /protein_id="HGU4550929.1"
                     /translation="PYIMKTTGLDFQQCSAFTEHAVFNYLCRVANERIFYARGNNRNI
                     DENILRQIKKIVPHPDTALHAYCSGQSKKRDSPWGLIFPFGLNESQLLAVERAFSSQV
                     SVIEGPPGTGKTQTILNIVANILIQNKTVAILSNNNSAVSNVYEKMDKQQLGYVVARL
                     GSTENRQQFFSTSISRSEEVLPDSPSANAIDDVLQQVKKHLNAINQVASLKAEINELN
                     IEYKYLQQWQSQNLRPEELFSHKYRLSSRKTTDLMAYIHYLSDRRIGFRNRIDLLLNF
                     RILKVKPLVIPERRLALFTSLQLSYYEKFIREKQVSLNEYEEVLKESDFKILLERLTS
                     WSVLYLKQHLRRNIST"
BASE COUNT          307 a          217 c          196 g          333 t
ORIGIN      
        1 ttgttgaaat atttcttcga agatgttgtt ttaaataaag cacagaccat gatgtcaggc
       61 gctccagtaa aattttaaaa tcagattcct ttaatacttc ctcatattca ttcagagaaa
      121 cttgtttttc ccggatgaat ttttcataat aagaaagctg aagagaagta aatagtgcca
      181 gccttctctc cggaatcacc aatggtttga ctttcagtat cctgaagttt aacaacaaat
      241 caattctgtt tctgaatcca atccgcctgt cagaaaggta atgaatataa gccatcaggt
      301 cagttgtttt tcgtgaggaa agtcggtatt tgtgagaaaa aagctcctcc ggtcgaagat
      361 tctgactctg ccattgctgc aggtatttat actcaatatt cagctcatta atttctgctt
      421 ttaacgaagc gacctggtta atggcattca ggtgtttttt cacttgttga agtacatcat
      481 caatcgcatt tgcagacggt gagtcaggta agacttcctc agaacgcgaa atcgacgttg
      541 aaaaaaactg ttgcctgttc tccgtactcc ccagtcgtgc caccacatat cccagttgct
      601 gtttgtccat cttttcataa acattactga cagctgagtt attattggac aaaattgcca
      661 ctgttttatt ctgaattaat atattagcaa caatattgag tatagtctgt gttttacctg
      721 ttccaggtgg tccttcaata actgaaacct gtgaggaaaa tgcccgttcg acagccagca
      781 actgactttc attaagacca aacggaaaaa tcaaacccca tggcgaatca cgttttttac
      841 tctgtcctga acagtatgca tgcaaggcgg tatccggatg aggaactatt ttctttatct
      901 gcctaaggat attctcatcg atatttctgt tattaccgcg ggcataaaaa atacgttcat
      961 tagccaccct acacaagtaa ttaaacacgg catgttctgt aaatgctgaa cactgttgaa
     1021 agtcaagacc ggtcgttttc atgatgtagg gtc
//
LOCUS       DAZKHF020000580         1050 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2201S31241
            SAMN43267800-rid25106413.denovo.584, whole genome shotgun sequence.
ACCESSION   DAZKHF020000580 DAZKHF020000000
VERSION     DAZKHF020000580.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267800
            Sequence Read Archive: SRR30307263
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1050)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1050)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 1050)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350581.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350581.2
            Genome Coverage       :: 151x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:21:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,652
            CDSs (total)                      :: 5,557
            Genes (coding)                    :: 5,320
            CDSs (with protein)               :: 5,320
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 237
            CDSs (without protein)            :: 237
            Pseudo Genes (ambiguous residues) :: 0 of 237
            Pseudo Genes (frameshifted)       :: 102 of 237
            Pseudo Genes (incomplete)         :: 130 of 237
            Pseudo Genes (internal stop)      :: 52 of 237
            Pseudo Genes (multiple problems)  :: 41 of 237
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1050
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267800-rid25106413.denovo.584"
                     /serotype="O 26:H11"
                     /isolate="2201S31241"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-01"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            complement(<1..951)
                     /locus_tag="ACHOSC_RS26000"
                     /old_locus_tag="pgaptmp_005200"
     CDS             complement(<1..951)
                     /locus_tag="ACHOSC_RS26000"
                     /old_locus_tag="pgaptmp_005200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000263438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HGU4550930.1"
                     /translation="MRNNGYYCYRDPRTGKEFGLGRDRRIAITEAIQANIELLSGNRR
                     ESLIDRIKGADAITLHAWLDRYETILSERGIRPKTLLDYASKIRAIRRKLPDKPLADI
                     STKEVAAMLNTYVAEGKAASAKLIRSTLVDVFREAIAEGHVATNPVTATRTAKSEVRR
                     SRLTANEYVAIYHAAEPLPIWLRLAMDLAVVTGQRVGDLCRMKWSDINDNHLHIEQGK
                     TGAKLAIPLTLTIDALNISLADTLQKCREASGSETIIASTHHEPLSPKTVSKYFTKAR
                     NASGLSFDGDPPTFHELRSLSARLYRNQIGDKFAQRLLGHK"
BASE COUNT          214 a          263 c          243 g          330 t
ORIGIN      
        1 tttatgcccg agaagacgtt gagcaaactt atcgccaatc tgattccggt atagcctcgc
       61 cgacaggctg cgcagttcat ggaatgttgg cgggtctcca tcaaatgaga gtccagatgc
      121 atttctcgcc tttgtaaaat acttagatac tgttttcggg gaaagcggtt cgtgatgcgt
      181 tgatgcaatt attgtttcac tgccgctggc ctccctgcat ttctgtagtg tatcagccaa
      241 tgagatattg agcgcgtcaa tcgttagcgt cagcggaatg gcgagcttag cccctgtttt
      301 accctgctca atgtgaagat ggttgtcgtt tatgtctgac catttcattc tgcataaatc
      361 gcctactctc tgccctgtaa cgacagccaa atccattgcc agcctcagcc agattgggag
      421 cggttcggct gcatggtaaa tcgcgacata ttcattagct gtcagtcttg aacgccttac
      481 ttctgatttt gctgtgcggg ttgctgttac cggattcgta gccacatgcc cctcggctat
      541 tgcctcacga aaaacgtcaa caagggttga cctgattaat tttgcggaag ccgctttacc
      601 ttctgctacg taggtgttta gcattgctgc cacctctttc gttgatatgt cagcgagcgg
      661 tttgtccggc aattttcttc ggattgccct gattttgctg gcgtagtcga gtagagtttt
      721 cggcctgata cccctctcgc tgaggattgt ttcatatcgg tcaagccacg catgaagagt
      781 gattgcgtca gcgcctttaa ttctgtctat cagtgactca cgcctgttcc cggatagcaa
      841 ctcaatattg gcctgtattg cttcagtgat tgctatcctc ctgtctcggc ctaatccgaa
      901 ctctttaccc gtccttgggt ccctgtagca gtaatatcca ttgtttctta tataaaggtt
      961 agggggtaaa tcccggcgct catgacttcg ccttcttccc atttctgatc ctcttcaaaa
     1021 ggctacttgt tactggtcga tttaagtcaa
//
LOCUS       DAZKHF020000581         1047 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2201S31241
            SAMN43267800-rid25106413.denovo.585, whole genome shotgun sequence.
ACCESSION   DAZKHF020000581 DAZKHF020000000
VERSION     DAZKHF020000581.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267800
            Sequence Read Archive: SRR30307263
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1047)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1047)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 1047)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350581.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350581.2
            Genome Coverage       :: 151x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:21:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,652
            CDSs (total)                      :: 5,557
            Genes (coding)                    :: 5,320
            CDSs (with protein)               :: 5,320
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 237
            CDSs (without protein)            :: 237
            Pseudo Genes (ambiguous residues) :: 0 of 237
            Pseudo Genes (frameshifted)       :: 102 of 237
            Pseudo Genes (incomplete)         :: 130 of 237
            Pseudo Genes (internal stop)      :: 52 of 237
            Pseudo Genes (multiple problems)  :: 41 of 237
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1047
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267800-rid25106413.denovo.585"
                     /serotype="O 26:H11"
                     /isolate="2201S31241"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-01"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            1..1047
                     /locus_tag="ACHOSC_RS26005"
                     /old_locus_tag="pgaptmp_005201"
                     /pseudo
     CDS             1..1047
                     /locus_tag="ACHOSC_RS26005"
                     /old_locus_tag="pgaptmp_005201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309822.1"
                     /note="frameshifted; incomplete; missing N-terminus and
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
BASE COUNT          251 a          246 c          333 g          217 t
ORIGIN      
        1 accacgctgc tgagaatgga cagcgaacgg cgcatgaaaa cactgggtga atcggtgaaa
       61 aatacgcttc tcacgcccaa tgaggcccgt aaacgtgaga acctgccgcc ccttgccggc
      121 ggtgatgcac tgtatcttca gcagcagaac tacagtctgg aggcgctgtc ccgtcgtgat
      181 gcccgtgagg acccgttcgc gtcggccggg aaaacagttt cagcacaact gcctgacggc
      241 gcatctgacg gtaataaggc aatcagtgaa acagagcatg atgcagtgaa agcgatgttc
      301 agggggatac tgagaaaatg acggaacggg aactgtccat tattcgtgca ctgggcgaag
      361 aattctccac ggtgctggcg gatttacagc gcacatttga ggggaagatg gccgcgcagg
      421 cacaagcgtt tgaagagaaa ctggcttccc tgtcggcggt attacagaag catgtgacgg
      481 tggatgaggt acatccggtt ctgcaggcga tggtggatga cgctgtgggg accattccgg
      541 taccgcgtga tggtcgtgat tatgatccgg atgtactgca gcaggcggtg aatgatgcgg
      601 ttgcgaatat tccggtaccg gcggacggca aaagtatcac ccccgatgat gtgcgtccga
      661 tgcttgagca gatggtgaaa gaggctgtaa gccatatccc tgctccgcgt gacggtcgtg
      721 attatgatcc ggaagtactg aagcaggcgg tgaatgatgc ggtcgcaaat attccggtac
      781 cggcggacgg caaaagtatc acccccgatg atgtgcgtcc gatgcttgag cagatggtga
      841 aagaggctgt aagccatatc cctgctccgc gtgacggttg tgattatgat ccggaagtac
      901 tgaagcaggc ggtgaatgat gcggttgcga atattccggt accggcggac ggcaaaagta
      961 tcacccccga tgatgtgcgt ccgatgcttg agcagatggt gaaagaggcc gtaagccata
     1021 tccctgctcc gcgtgatggt cgtgatt
//
LOCUS       DAZKHF020000582         1035 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2201S31241
            SAMN43267800-rid25106413.denovo.586, whole genome shotgun sequence.
ACCESSION   DAZKHF020000582 DAZKHF020000000
VERSION     DAZKHF020000582.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267800
            Sequence Read Archive: SRR30307263
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1035)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1035)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 1035)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350581.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350581.2
            Genome Coverage       :: 151x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:21:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,652
            CDSs (total)                      :: 5,557
            Genes (coding)                    :: 5,320
            CDSs (with protein)               :: 5,320
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 237
            CDSs (without protein)            :: 237
            Pseudo Genes (ambiguous residues) :: 0 of 237
            Pseudo Genes (frameshifted)       :: 102 of 237
            Pseudo Genes (incomplete)         :: 130 of 237
            Pseudo Genes (internal stop)      :: 52 of 237
            Pseudo Genes (multiple problems)  :: 41 of 237
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1035
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267800-rid25106413.denovo.586"
                     /serotype="O 26:H11"
                     /isolate="2201S31241"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-01"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            <1..185
                     /locus_tag="ACHOSC_RS26010"
                     /old_locus_tag="pgaptmp_005202"
     CDS             <1..185
                     /locus_tag="ACHOSC_RS26010"
                     /old_locus_tag="pgaptmp_005202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4550931.1"
                     /translation="KVAIRTIDRHVGEGYAKEHPDLISAFMTTAAANFATLTEREIAE
                     AEQVTTINVKTGEVES"
     gene            182..487
                     /locus_tag="ACHOSC_RS26015"
                     /old_locus_tag="pgaptmp_005203"
     CDS             182..487
                     /locus_tag="ACHOSC_RS26015"
                     /old_locus_tag="pgaptmp_005203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001294167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4550932.1"
                     /translation="MSEPKFGEKLYKHNGRITIQQISAATPGWWVETDEGSSPVASWA
                     LCVLSYPDRDVYQDILPVISTDKGMKPVDIKNMGFQCVMLTEKMMEEMKKNNAGSLH"
     gene            497..907
                     /locus_tag="ACHOSC_RS26020"
                     /old_locus_tag="pgaptmp_005204"
     CDS             497..907
                     /locus_tag="ACHOSC_RS26020"
                     /old_locus_tag="pgaptmp_005204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HGU4550933.1"
                     /translation="MTAQIAAYGRLVDDPLVKQSSKGTPMTLARMAVSLPCSQAHDGQ
                     ATLWLSVIAFGKQADFLAKHQKGDVASVSGTMQVSQWTGQNGETRQGYQVIADSVISA
                     RAARPGGNRRKTTGTQGNQPPAGGDDPYGDDIPF"
     gene            920..>1035
                     /locus_tag="ACHOSC_RS26025"
                     /old_locus_tag="pgaptmp_005205"
     CDS             920..>1035
                     /locus_tag="ACHOSC_RS26025"
                     /old_locus_tag="pgaptmp_005205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016241305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PerC family transcriptional regulator"
                     /protein_id="HGU4550934.1"
                     /translation="MVHDRIAEELEAKGFYRRASARWGEVMQLVETDKERHH"
BASE COUNT          306 a          239 c          309 g          181 t
ORIGIN      
        1 taaaggtggc aatcagaacg atagaccgac acgtagggga aggatacgcg aaggaacatc
       61 ccgacctgat aagcgcattc atgaccacag cagcggcaaa ctttgccacg ctgacagaac
      121 gggagattgc cgaagcggaa caggtaacaa ccatcaacgt taaaaccgga gaggtggaat
      181 catgagcgaa ccaaaatttg gtgaaaaact ttacaagcac aacggacgga tcacgattca
      241 gcaaataagc gcagctacac caggctggtg ggttgaaaca gacgaaggat catcacccgt
      301 ggcatcatgg gcgctttgtg tgcttagtta ccctgaccgc gatgtgtatc aggatattct
      361 gcctgtgata tccactgaca aaggcatgaa acccgtagac atcaaaaaca tgggttttca
      421 gtgcgtaatg ctcactgaaa aaatgatgga agagatgaaa aagaacaacg ccggatcgct
      481 tcactgaggt ggcaatatga cagcacaaat tgcagcttac gggcgcctgg tggacgaccc
      541 gctggtaaaa cagagcagca agggcacacc gatgactctg gcgcgtatgg cggtatcgtt
      601 gccatgcagc caggcacatg acggacaggc gacgttatgg ttatcggtta tcgcatttgg
      661 caaacaggcc gatttcctgg ctaaacatca aaaaggcgat gttgccagcg tatccggcac
      721 gatgcaggta agccagtgga ccggacagaa cggggaaaca cggcaggggt atcaggttat
      781 tgcagacagc gtaatcagtg cccgtgcggc acgtcctggc gggaacagac gcaaaaccac
      841 aggcacacag ggtaatcagc caccagcggg aggcgatgac ccttacggtg atgatattcc
      901 gttctgaggg gtggggacga tggtacatga ccgcatagcg gaagagctgg aggcgaaagg
      961 cttttaccgg agagcgtcgg cgcgatgggg tgaagtcatg cagctggtgg agacagacaa
     1021 ggaacggcat cacat
//
LOCUS       DAZKHF020000583         1026 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2201S31241
            SAMN43267800-rid25106413.denovo.587, whole genome shotgun sequence.
ACCESSION   DAZKHF020000583 DAZKHF020000000
VERSION     DAZKHF020000583.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267800
            Sequence Read Archive: SRR30307263
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1026)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1026)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 1026)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350581.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350581.2
            Genome Coverage       :: 151x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:21:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,652
            CDSs (total)                      :: 5,557
            Genes (coding)                    :: 5,320
            CDSs (with protein)               :: 5,320
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 237
            CDSs (without protein)            :: 237
            Pseudo Genes (ambiguous residues) :: 0 of 237
            Pseudo Genes (frameshifted)       :: 102 of 237
            Pseudo Genes (incomplete)         :: 130 of 237
            Pseudo Genes (internal stop)      :: 52 of 237
            Pseudo Genes (multiple problems)  :: 41 of 237
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1026
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267800-rid25106413.denovo.587"
                     /serotype="O 26:H11"
                     /isolate="2201S31241"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-01"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            complement(<1..1024)
                     /gene="ompC"
                     /locus_tag="ACHOSC_RS26030"
                     /old_locus_tag="pgaptmp_005206"
     CDS             complement(<1..1024)
                     /gene="ompC"
                     /locus_tag="ACHOSC_RS26030"
                     /old_locus_tag="pgaptmp_005206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_process: GO:0034220 - monoatomic ion transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="porin OmpC"
                     /protein_id="HGU4550935.1"
                     /translation="MMAMSAQAAEIYNKDSNKLDLYGKVNAKHYFSSNDADDGDTTYA
                     RLGFKGETQINDQLTGFGQWEYEFKGNRAESQGSSKDKTRLAFAGLKFGDYGSIDYGR
                     NYGVAYDIGAWTDVLPEFGGDTWTQTDVFMTGRTTGVATYRNNDFFGLVDGLNFAAQY
                     QGKNDRTDVTQANGDGFGFSTTYEYEGFGVGATYAKSDRTNNQVIYGNNSLNASGQNA
                     EVWAAGLKYDANNIYLATTYSETQNMTVFGKNHIANKAQNFEVVAQYQFDFGLRPSVA
                     YLQSKGKDLGAWGDQDLVEYIDVGATYYFNKNMSTFVDYKINLIDKSDFTKASGVATD
                     DIVAVGM"
BASE COUNT          287 a          234 c          216 g          289 t
ORIGIN      
        1 ccatacctac agcaacgata tcatcggtag caacgccaga tgctttcgtg aaatcgctct
       61 tatcaatcag gttgattttg tagtcaacaa aagtggacat atttttgttg aagtaatagg
      121 ttgcacctac atcaatatat tcaaccaggt cctgatcacc ccaagcaccc aagtctttcc
      181 ctttagattg cagataagca acggacggac gcagaccgaa gtcgaactga tattgtgcaa
      241 ctacttcgaa gttttgtgct ttgttggcaa tatggttttt accaaaaaca gtcatgtttt
      301 gggtttcaga ataggtagta gccagataga tgttgttcgc atcatatttc agaccagctg
      361 cccatacttc agcattttga ccagatgcat tcaggctgtt gttaccgtag ataacctgat
      421 tattagtgcg atcagattta gcataggttg cacctacgcc gaaaccttca tactcataag
      481 tagtggagaa accgaaacca tcaccattag cttgagttac gtcagtacgg tcatttttac
      541 cctgatactg agcagcaaag ttcagaccat caaccagacc aaagaagtca ttgttacgat
      601 aagttgcaac acctgtggtg cgaccagtca tgaatacatc tgtttgggtc caggtatcgc
      661 caccgaattc tggcagaacg tcggtccatg caccaatatc gtatgctaca ccgtagttac
      721 ggccataatc gatggagccg tagtcaccga atttcaggcc agcgaaggca agacgggttt
      781 tatctttgga ggaaccttga gattcagcgc ggttgccttt gaattcatat tcccactgac
      841 cgaaaccagt cagttgatcg ttgatttggg tttcaccttt gaagccaaga cgggcataag
      901 tagtatcacc atcatctgca tcattagagg agaagtagtg cttagcatta actttcccgt
      961 acagatccag cttgttactg tctttattat aaatttcagc tgcctgagca gacatcgcca
     1021 tcagta
//
LOCUS       DAZKHF020000584         1009 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2201S31241
            SAMN43267800-rid25106413.denovo.588, whole genome shotgun sequence.
ACCESSION   DAZKHF020000584 DAZKHF020000000
VERSION     DAZKHF020000584.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267800
            Sequence Read Archive: SRR30307263
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1009)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1009)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 1009)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350581.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350581.2
            Genome Coverage       :: 151x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:21:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,652
            CDSs (total)                      :: 5,557
            Genes (coding)                    :: 5,320
            CDSs (with protein)               :: 5,320
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 237
            CDSs (without protein)            :: 237
            Pseudo Genes (ambiguous residues) :: 0 of 237
            Pseudo Genes (frameshifted)       :: 102 of 237
            Pseudo Genes (incomplete)         :: 130 of 237
            Pseudo Genes (internal stop)      :: 52 of 237
            Pseudo Genes (multiple problems)  :: 41 of 237
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1009
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267800-rid25106413.denovo.588"
                     /serotype="O 26:H11"
                     /isolate="2201S31241"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-01"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            <1..>1009
                     /locus_tag="ACHOSC_RS26035"
                     /old_locus_tag="pgaptmp_005207"
     rRNA            <1..>1009
                     /locus_tag="ACHOSC_RS26035"
                     /old_locus_tag="pgaptmp_005207"
                     /product="23S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF02541"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF02541"
BASE COUNT          239 a          229 c          319 g          222 t
ORIGIN      
        1 cgaagccccg gtaaacggcg gccgtaacta taacggtcct aaggtagcga aattccttgt
       61 cgggtaagtt ccgacctgca cgaatggcgt aatgatggcc aggctgtctc cacccgagac
      121 tcagtgaaat tgaactcgct gtgaagatgc agtgtacccg cggcaagacg gaaagacccc
      181 gtgaaccttt actatagctt gacactgaac attgagcctt gatgtgtagg ataggtggga
      241 ggctttgaag tgtggacgcc agtctgcatg gagccgacct tgaaatacca ccctttaatg
      301 tttgatgttc taacgttgac ccgtaatccg ggttgcggac agtgtctggt gggtagtttg
      361 actggggcgg tctcctccta aagagtaacg gaggagcacg aaggttggct aatcctggtc
      421 ggacatcagg aggttagtgc aatggcataa gccagcttga ctgcgagcgt gacggcgcga
      481 gcaggtgcga aagcaggtca tagtgatccg gtggttctga atggaagggc catcgctcaa
      541 cggataaaag gtactccggg gataacaggc tgataccgcc caagagttca tatcgacggc
      601 ggtgtttggc acctcgatgt cggctcatca catcctgggg ctgaagtagg tcccaagggt
      661 atggctgttc gccatttaaa gtggtacgcg agctgggttt agaacgtcgt gagacagttc
      721 ggtccctatc tgccgtgggc gctggagaac tgaggggggc tgctcctagt acgagaggac
      781 cggagtggac gcatcactgg tgttcgggtt gtcatgccaa tggcactgcc cggtagctaa
      841 atgcggaaga gataagtgct gaaagcatct aagcacgaaa cttgccccga gatgagttct
      901 ccctgactcc ttgagagtcc tgaaggaacg ttgaagacga cgacgttgat aggccgggtg
      961 tgtaagcgca gcgatgcgtt gagctaaccg gtactaatga accgtgagg
//
LOCUS       DAZKHF020000585         1007 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2201S31241
            SAMN43267800-rid25106413.denovo.589, whole genome shotgun sequence.
ACCESSION   DAZKHF020000585 DAZKHF020000000
VERSION     DAZKHF020000585.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267800
            Sequence Read Archive: SRR30307263
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1007)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1007)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 1007)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350581.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350581.2
            Genome Coverage       :: 151x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:21:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,652
            CDSs (total)                      :: 5,557
            Genes (coding)                    :: 5,320
            CDSs (with protein)               :: 5,320
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 237
            CDSs (without protein)            :: 237
            Pseudo Genes (ambiguous residues) :: 0 of 237
            Pseudo Genes (frameshifted)       :: 102 of 237
            Pseudo Genes (incomplete)         :: 130 of 237
            Pseudo Genes (internal stop)      :: 52 of 237
            Pseudo Genes (multiple problems)  :: 41 of 237
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1007
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267800-rid25106413.denovo.589"
                     /serotype="O 26:H11"
                     /isolate="2201S31241"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-01"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            <1..>1007
                     /locus_tag="ACHOSC_RS26040"
                     /old_locus_tag="pgaptmp_005208"
     CDS             <1..>1007
                     /locus_tag="ACHOSC_RS26040"
                     /old_locus_tag="pgaptmp_005208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="RHS repeat-associated core domain-containing
                     protein"
                     /protein_id="HGU4550936.1"
                     /translation="GRLTEKTDLIPEGVIRTDDERTHQYHYDSQHRLVHYTRTQYAEP
                     LVESRYLYDPLGRRVAKRVWRRERDLTGWMSLSRKPEVTWYGWDGDRLTTIQNDRTRI
                     QTIYQPGSFTPLIRVETATGELAKTQRRSLADTLQQSGGEDGGSVVFPPVLVQMLDRL
                     ESEILADRVSEESRRWLASCGLTVEQMQNQMDPVYTPARKIHLYHCDHRGLPLALISK
                     EGATEWCAEYDEWGNLLNEENPHQLQQLIRLPGQQYDEESGLYYNRHRYYDPLQGRYI
                     TQDPIGLKGGWNLYGYQLNPISDIDPLGLSMWEDAKSGACTNGLCGTLSAMIGPDKFD
                     S"
BASE COUNT          243 a          273 c          313 g          178 t
ORIGIN      
        1 acggcaggct gacagagaaa accgacctca tcccggaagg ggttatccgc acggatgatg
       61 agcgcaccca ccagtaccat tacgacagtc agcaccggct ggtgcactac acgcggacac
      121 aatatgcaga gccgctggtc gaaagtcgct atctttacga cccgctgggc cgcagggtgg
      181 caaaacgggt atggcgacgt gaacgggacc tgacgggctg gatgtcgctg tcacggaaac
      241 cggaagtgac ctggtacggc tgggacggcg accggctgac cacgatacag aacgacagaa
      301 cccgcatcca gacgatttat cagccgggga gcttcacgcc actcatcaga gttgaaaccg
      361 ccaccggtga gctggcgaaa acgcagcgcc gcagcctggc ggataccctt cagcagtccg
      421 gcggcgaaga cggtggcagt gtggtgttcc cgccggtgct ggtgcagatg ctcgaccggc
      481 tggaaagtga aatcctggct gaccgggtga gtgaggaaag ccgccgctgg ctggcatcgt
      541 gcggcctgac tgtggagcag atgcaaaacc agatggaccc ggtgtacacg ccggcgcgaa
      601 aaatccacct gtaccactgc gaccatcgcg gcctgccgct ggcccttatc agtaaggaag
      661 gggcaacaga atggtgcgca gaatacgatg agtggggcaa cctgctgaat gaagagaacc
      721 cgcatcagct gcagcagctt atccgcctgc cggggcagca gtatgatgag gagtccggcc
      781 tgtattacaa ccgccaccgc tattatgacc cgctgcaggg gcgatatatc actcaggatc
      841 cgattggact gaagggggga tggaacctgt atggatatca attgaatccg atatcagaca
      901 tcgaccccct gggtttatct atgtgggagg atgcaaaatc gggggcatgt actaatggtc
      961 tttgcggcac actatccgct atgataggtc cagataaatt tgattct
//
LOCUS       DAZKHF020000586         1005 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2201S31241
            SAMN43267800-rid25106413.denovo.590, whole genome shotgun sequence.
ACCESSION   DAZKHF020000586 DAZKHF020000000
VERSION     DAZKHF020000586.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267800
            Sequence Read Archive: SRR30307263
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1005)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1005)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 1005)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350581.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350581.2
            Genome Coverage       :: 151x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:21:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,652
            CDSs (total)                      :: 5,557
            Genes (coding)                    :: 5,320
            CDSs (with protein)               :: 5,320
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 237
            CDSs (without protein)            :: 237
            Pseudo Genes (ambiguous residues) :: 0 of 237
            Pseudo Genes (frameshifted)       :: 102 of 237
            Pseudo Genes (incomplete)         :: 130 of 237
            Pseudo Genes (internal stop)      :: 52 of 237
            Pseudo Genes (multiple problems)  :: 41 of 237
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1005
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267800-rid25106413.denovo.590"
                     /serotype="O 26:H11"
                     /isolate="2201S31241"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-01"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            <1..>1005
                     /locus_tag="ACHOSC_RS26045"
                     /old_locus_tag="pgaptmp_005209"
     CDS             <1..>1005
                     /locus_tag="ACHOSC_RS26045"
                     /old_locus_tag="pgaptmp_005209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="3'-5' exoribonuclease"
                     /protein_id="HGU4550937.1"
                     /translation="LSSPVPETDAVETAIPGNEKTECKVEVEPSVEREGPFYFLFTDK
                     DGEKYGRANKLSGLEKALALGATEITKEEYFARKNGTYSGSQQNTGASDTTAQPEPVK
                     VTADEVNKIMQAANISQPDADKLLAASRGEFVAGISDPNDPKWVKGIETRDSVNQNQQ
                     ESEQNDQKAEQNSPNTQQNEPETKQPEPVAQQEPEKVCTACGQTGGGNCPDCGAVMGD
                     ATYQETFDEEYQVEVQEDDPEEMEGAEHPHKENTDGNQHHDSDNETGETADHSIKVNG
                     HQEITSTSRTCDHLMIDLETMGKNPDAPIISIGAIFFDPQTGDMGPEFSKTIDLETAG
                     G"
BASE COUNT          335 a          243 c          257 g          170 t
ORIGIN      
        1 tctctcgtcg ccagtaccag aaactgatgc agtggaaacg gcaattccgg gcaacgaaaa
       61 aaccgaatgc aaagtggaag tcgaaccatc tgtagagcgt gaggggccgt tctacttcct
      121 cttcaccgac aaggatggcg aaaaatacgg tcgcgcaaac aaactttctg gtctggaaaa
      181 agcactagcc ttgggagcta cggaaatcac aaaagaggaa tacttcgcac gtaaaaacgg
      241 cacgtactca ggttcacaac aaaatactgg tgcatctgac acgaccgcac aaccagagcc
      301 ggtaaaagtt accgctgacg aagtaaacaa aattatgcag gcagccaata tcagccagcc
      361 tgacgccgat aagttgcttg ctgcctctcg cggagaattt gttgcaggga ttagcgaccc
      421 gaatgatccg aaatgggtaa aggggattga aacccgcgat tctgtaaacc agaaccagca
      481 agaatcggaa cagaacgacc agaaagcgga acaaaacagc ccaaatacgc aacaaaacga
      541 gccagaaacg aaacaacctg agccagtagc gcaacaggaa ccggaaaaag tctgcaccgc
      601 ctgcggtcag accggcggcg gcaactgccc tgattgtggc gcggtgatgg gcgacgcaac
      661 ataccaggaa acattcgatg aagagtatca ggttgaagtt caggaagatg atccggagga
      721 aatggaaggc gctgaacatc cacacaagga gaacactgac ggcaatcagc atcacgatag
      781 cgataatgaa actggcgaga cggcagatca ctcaattaag gtgaacggtc atcaagaaat
      841 cacatccacc agcaggacgt gtgaccatct aatgatcgac cttgaaacca tgggaaaaaa
      901 tcctgatgcc ccgatcatct caataggtgc aatatttttc gatccgcaaa ccggagatat
      961 ggggccggaa tttagtaaga ctatcgatct ggaaactgct ggcgg
//
LOCUS       DAZKHF020000587         1004 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2201S31241
            SAMN43267800-rid25106413.denovo.591, whole genome shotgun sequence.
ACCESSION   DAZKHF020000587 DAZKHF020000000
VERSION     DAZKHF020000587.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267800
            Sequence Read Archive: SRR30307263
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1004)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1004)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 1004)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350581.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350581.2
            Genome Coverage       :: 151x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:21:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,652
            CDSs (total)                      :: 5,557
            Genes (coding)                    :: 5,320
            CDSs (with protein)               :: 5,320
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 237
            CDSs (without protein)            :: 237
            Pseudo Genes (ambiguous residues) :: 0 of 237
            Pseudo Genes (frameshifted)       :: 102 of 237
            Pseudo Genes (incomplete)         :: 130 of 237
            Pseudo Genes (internal stop)      :: 52 of 237
            Pseudo Genes (multiple problems)  :: 41 of 237
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1004
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267800-rid25106413.denovo.591"
                     /serotype="O 26:H11"
                     /isolate="2201S31241"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-01"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            3..866
                     /locus_tag="ACHOSC_RS26050"
                     /old_locus_tag="pgaptmp_005210"
     CDS             3..866
                     /locus_tag="ACHOSC_RS26050"
                     /old_locus_tag="pgaptmp_005210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710010.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjeJ family protein"
                     /protein_id="HGU4550938.1"
                     /translation="MSIKGVNTGVIRKSNNFIALALKIKEPRNKESLFFMSVMELRDL
                     LIALESRMHQKHKLDAAARLQYEQARDKVIKKMAENIPEILVDELKNADINRRVNTLE
                     LTDNQGENLTFVLTLHDGSKCELVVNELQIEMLARAIIHAINNAEMRELALRITSLLD
                     FLPLYDVDCQENGNLEYDTYSQPEWKHNLFDHYLAVLYRFKDESGKEQFSGAVVKTRE
                     ATPGKEIEAITRRMLDFSQRLKKLAGVPCQVYVRTVAANNAQPLTQDQCLRALHHLRV
                     QSTSKTAPQAK"
BASE COUNT          300 a          228 c          231 g          245 t
ORIGIN      
        1 ccataagcat caagggtgtt aataccggtg ttattcgcaa gagtaataat ttcattgcac
       61 tggcgctgaa aattaaagaa cctcgcaata aagaatcgtt gttctttatg tctgtcatgg
      121 aactgcgcga cctgctgatt gcgctggaaa gtcgtatgca tcaaaagcat aaactggatg
      181 ctgctgcacg ccttcagtat gagcaagctc gcgataaagt cattaagaaa atggcagaaa
      241 acatcccaga aattctggtt gatgagctta aaaatgccga tattaatcgt cgggttaaca
      301 cgctggaact taccgataat caaggcgaaa atcttacctt cgtattaacg ctacatgacg
      361 gtagcaaatg tgagttagtc gtcaatgagt tgcagattga aatgctggcg cgagcaatca
      421 ttcatgcaat taataatgcg gaaatgcgtg aactggcatt acgtattacc tcactgttag
      481 atttcctgcc gttatatgac gttgactgcc aggaaaatgg caatctggaa tatgacacct
      541 attcccaacc ggaatggaaa cataacctgt tcgaccatta tctggcggtg ctttaccgct
      601 ttaaagatga aagtggcaaa gaacaattta gcggcgcagt ggtgaaaacg cgtgaagcaa
      661 cgccgggtaa agaaatagag gcgatcactc gccgaatgct tgatttcagc caacgactga
      721 aaaaactcgc gggtgtgcca tgtcaggtct atgttcgcac cgtagcggcg aataacgccc
      781 aaccgctaac ccaggatcaa tgcttacgcg cgctgcatca cttacgtgtt caatccacca
      841 gcaaaactgc accacaggcg aaataatctc atctttgcta atgcgaggct ggagccatcc
      901 agcctgaaat gactgataac gtgatcgttg gtcatgccgg atgcggcgta aacgccttat
      961 ccggcctaca aattctcaag cattcaatca gttgcaatgc ctgc
//
LOCUS       DAZKHF020000588          996 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2201S31241
            SAMN43267800-rid25106413.denovo.592, whole genome shotgun sequence.
ACCESSION   DAZKHF020000588 DAZKHF020000000
VERSION     DAZKHF020000588.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267800
            Sequence Read Archive: SRR30307263
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 996)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 996)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 996)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350581.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350581.2
            Genome Coverage       :: 151x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:21:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,652
            CDSs (total)                      :: 5,557
            Genes (coding)                    :: 5,320
            CDSs (with protein)               :: 5,320
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 237
            CDSs (without protein)            :: 237
            Pseudo Genes (ambiguous residues) :: 0 of 237
            Pseudo Genes (frameshifted)       :: 102 of 237
            Pseudo Genes (incomplete)         :: 130 of 237
            Pseudo Genes (internal stop)      :: 52 of 237
            Pseudo Genes (multiple problems)  :: 41 of 237
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..996
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267800-rid25106413.denovo.592"
                     /serotype="O 26:H11"
                     /isolate="2201S31241"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-01"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            <1..732
                     /locus_tag="ACHOSC_RS26055"
                     /old_locus_tag="pgaptmp_005211"
     CDS             <1..732
                     /locus_tag="ACHOSC_RS26055"
                     /old_locus_tag="pgaptmp_005211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="terminase TerL endonuclease subunit"
                     /protein_id="HGU4550939.1"
                     /translation="RFNIWCQGATPWMGNGAWAECAGTFTEEDLHGQECYAGLDLSST
                     SDISSVCYAFPVGKNIMLVSRHYLPEFQLQNPANKNRAVYRQWAKAGWIRTTPGDCID
                     YDRIRDDIMQDAEKFNIRLVGFDTWNATHLRTQLQGAGFEVEPFPQTYLRFSPAAKSF
                     EVFVNRRVIVHRGDPVLSWSMSNVVMQSDANANIKPNKKKSPNKIDPSVAALMAFGTF
                     QAEHEDFAFDISDSHRQKLEEFSGV"
     gene            746..>996
                     /locus_tag="ACHOSC_RS26060"
                     /old_locus_tag="pgaptmp_005212"
     CDS             746..>996
                     /locus_tag="ACHOSC_RS26060"
                     /old_locus_tag="pgaptmp_005212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4550940.1"
                     /translation="MTEAEILRLIRRVSGISQQADEQTTQPDSVTAENYARVVAEVMR
                     RDGIQLNDADMRDIRIRVLEMLAYNRRVELYREKEKITY"
BASE COUNT          252 a          234 c          294 g          216 t
ORIGIN      
        1 cgatttaata tctggtgcca gggggctacc ccgtggatgg gtaacggcgc atgggctgag
       61 tgtgccggaa cgttcacgga ggaagattta cacgggcagg agtgttacgc ggggctggac
      121 ttatcatcaa ccagcgatat ttccagcgtg tgctatgcct ttccggtcgg taaaaatatc
      181 atgctggttt cccgtcatta tctgccggag ttccagctac agaaccccgc caataaaaac
      241 cgcgctgtct atcgccagtg ggctaaagcg ggctggatac gcacaacgcc aggtgactgc
      301 attgattacg accggatcag agatgacatc atgcaggatg cggagaaatt taatatcagg
      361 ctggtgggct ttgatacgtg gaacgccacg catctcagga cgcaattaca gggggctggt
      421 tttgaggtgg agccgttccc gcaaacctac ctcagattca gtccggcggc gaaatcgttc
      481 gaagtttttg ttaaccgcag ggtgattgtg catcgtggcg atccggtgtt gtcctggtcg
      541 atgagtaacg tcgtgatgca gagtgacgcg aacgccaata tcaagccgaa caagaaaaaa
      601 tcaccgaaca agatagaccc gagcgtagcg gcgctgatgg cgtttggtac attccaggca
      661 gagcatgagg attttgcttt tgatataagc gacagccacc gccagaaact ggaagaattt
      721 agcggggtgt aatgaggtca gcagcatgac agaagccgaa atactgcgat taatccgccg
      781 cgtctctgga atcagccagc aggctgacga acagaccacg cagccggaca gcgtgacagc
      841 cgaaaattat gcgcgtgttg ttgctgaggt gatgcgccgt gatggtatcc agcttaatga
      901 tgcggatatg cgcgacatac ggatccgcgt tcttgaaatg ctggcctaca atcgccgcgt
      961 tgaactgtat cgggagaagg agaaaatcac gtacca
//
LOCUS       DAZKHF020000589          996 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2201S31241
            SAMN43267800-rid25106413.denovo.593, whole genome shotgun sequence.
ACCESSION   DAZKHF020000589 DAZKHF020000000
VERSION     DAZKHF020000589.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267800
            Sequence Read Archive: SRR30307263
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 996)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 996)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 996)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350581.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350581.2
            Genome Coverage       :: 151x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:21:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,652
            CDSs (total)                      :: 5,557
            Genes (coding)                    :: 5,320
            CDSs (with protein)               :: 5,320
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 237
            CDSs (without protein)            :: 237
            Pseudo Genes (ambiguous residues) :: 0 of 237
            Pseudo Genes (frameshifted)       :: 102 of 237
            Pseudo Genes (incomplete)         :: 130 of 237
            Pseudo Genes (internal stop)      :: 52 of 237
            Pseudo Genes (multiple problems)  :: 41 of 237
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..996
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267800-rid25106413.denovo.593"
                     /serotype="O 26:H11"
                     /isolate="2201S31241"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-01"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            <1..459
                     /locus_tag="ACHOSC_RS26065"
                     /old_locus_tag="pgaptmp_005213"
     CDS             <1..459
                     /locus_tag="ACHOSC_RS26065"
                     /old_locus_tag="pgaptmp_005213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4550941.1"
                     /translation="GMLEATQVRGDFVKAVSKAFPKKVGTWGNTETPNGTVTVTISDD
                     HNFDRQIIIPPIIFNGIAYDDPGSGNNPGGTRYTGYGFEVRKNGVLIASRETKGAIPG
                     SYSAVIDMPSGGGSVTLEFKIFQKGNQGAGNITDCTVIVTKKAASGISIR"
     gene            530..>996
                     /locus_tag="ACHOSC_RS26070"
                     /old_locus_tag="pgaptmp_005214"
     CDS             530..>996
                     /locus_tag="ACHOSC_RS26070"
                     /old_locus_tag="pgaptmp_005214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044384 - host outer membrane [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Ail/Lom family outer membrane beta-barrel
                     protein"
                     /protein_id="HGU4550942.1"
                     /translation="MRKLYAAILSAAICLVVSGAPAWASEHQSTLSAGYLHVSTNVPG
                     SDELNGINVKYRYEFTDTLGMVTSFSYAGDKNRQLTHYSDTRWHEDSVRNRWFSVMAG
                     PSVRVNEWFSAYAMAGVAYSRVSTFSGDYLQVTDNKGKTHDVLTGSDDGRHSNT"
BASE COUNT          259 a          226 c          280 g          231 t
ORIGIN      
        1 ggcatgctgg aggcgaccca ggtcagaggg gatttcgtta aagctgtatc aaaagccttc
       61 ccgaaaaaag tcggtacgtg gggtaacacg gaaacaccaa acggtacggt tacagtcacc
      121 atcagcgatg atcataactt tgaccgccag attattattc cgcccattat ttttaacggt
      181 atagcgtatg acgatccagg gagcggaaat aacccaggag gcacgcgata cacggggtat
      241 ggttttgaag ttcgcaaaaa cggcgtatta atcgcatcca gagaaactaa aggggccatt
      301 cccggtagtt acagtgcagt tattgatatg cctagtggtg gtggtagcgt cactctggag
      361 tttaagattt tccagaaagg caatcagggg gcaggcaata tcaccgactg tacggtgatt
      421 gtgaccaaaa aagctgcttc cggcatcagt attcgttgaa atatttataa ccccaataaa
      481 gggcgtcagg aatgacgcct tttttattgc agaaaagcga gaggtaatta tgcgtaaact
      541 ttatgccgcc attttgtccg cagccatctg tctggtcgta tccggtgcgc ctgcatgggc
      601 gtctgaacat cagtccacgc tgagcgcggg gtatcttcat gtctcgacga acgttcctgg
      661 cagcgatgaa ctgaacggga ttaacgtgaa ataccgttat gagtttacgg acacactggg
      721 gatggtgacg tcgttcagct atgcaggaga caagaatcgc cagctgaccc attacagcga
      781 tacccgctgg catgaagatt ccgttcgtaa ccgctggttc agcgtaatgg cggggccgtc
      841 tgtgcgcgtg aatgaatggt tcagcgcgta tgcgatggcg ggcgtggctt acagccgtgt
      901 gtcgactttc tccggagatt atctgcaggt gaccgacaac aaggggaaaa cgcatgatgt
      961 gctgaccgga agtgatgacg gccgccacag caacac
//
LOCUS       DAZKHF020000590          994 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2201S31241
            SAMN43267800-rid25106413.denovo.594, whole genome shotgun sequence.
ACCESSION   DAZKHF020000590 DAZKHF020000000
VERSION     DAZKHF020000590.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267800
            Sequence Read Archive: SRR30307263
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 994)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 994)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 994)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350581.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350581.2
            Genome Coverage       :: 151x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:21:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,652
            CDSs (total)                      :: 5,557
            Genes (coding)                    :: 5,320
            CDSs (with protein)               :: 5,320
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 237
            CDSs (without protein)            :: 237
            Pseudo Genes (ambiguous residues) :: 0 of 237
            Pseudo Genes (frameshifted)       :: 102 of 237
            Pseudo Genes (incomplete)         :: 130 of 237
            Pseudo Genes (internal stop)      :: 52 of 237
            Pseudo Genes (multiple problems)  :: 41 of 237
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..994
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267800-rid25106413.denovo.594"
                     /serotype="O 26:H11"
                     /isolate="2201S31241"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-01"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            complement(113..802)
                     /locus_tag="ACHOSC_RS26075"
                     /old_locus_tag="pgaptmp_005215"
     CDS             complement(113..802)
                     /locus_tag="ACHOSC_RS26075"
                     /old_locus_tag="pgaptmp_005215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriophage antitermination protein Q"
                     /protein_id="HGU4550943.1"
                     /translation="MNTQYLQYVREQLMAATADLNGATKGQLEAWQEHAQFDTGTYKR
                     KKPRILDVVTGKMITLDNTPTSGKQSYAKGSSIALVSPVEFSTSSWRRAVLSLDEHQK
                     AWLLWCYSESVRWGHQVTITQWAWSEFKDLLSNRKIAGKTLDRLKTLIWLAAQDVKSE
                     LAGREAYEYQTLASLVGVTTKNWSETFTERWVAMKHIFLQLDSDALLLVTRTRSKQKA
                     AFLQQNIAKLD"
     gene            complement(817..939)
                     /locus_tag="ACHOSC_RS26080"
                     /old_locus_tag="pgaptmp_005216"
     CDS             complement(817..939)
                     /locus_tag="ACHOSC_RS26080"
                     /old_locus_tag="pgaptmp_005216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000971054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlcG family protein"
                     /protein_id="HGU4550944.1"
                     /translation="MMSESYISELLRCRWGLLCLCRFPDSVLNDYRMLKNYAKI"
BASE COUNT          265 a          252 c          191 g          286 t
ORIGIN      
        1 gcagccagca tgtttaccgt acaagtacaa ctcagggcat aaaaaaaacc cactcggcag
       61 cgggtttata cattttttac aacataccaa atttgcatga agtatatggc ttttaatcca
      121 gttttgcaat attttgctgt aaaaatgctg ccttttgttt tgaacgtgtt ctcgtcacaa
      181 gcaataaagc atcactatca agctgtagaa aaatgtgctt cattgcaacc cagcgttcag
      241 taaatgtctc ggaccagttt tttgttgtca ctcccaccaa tgatgccagt gtctggtatt
      301 cataggcctc acgccctgca agttcgctct tcacatcctg tgcagccagc cagattaacg
      361 tcttcaggcg atccagtgtc ttacctgcaa tttttctgtt acttaacaaa tctttaaact
      421 cgctccatgc ccattgcgtt atggtgacct gatgccccca tcgaacgctt tcgctgtaac
      481 accaaagcaa ccatgctttc tgatgttcat cgagagacaa aaccgcgcgg cgccatgaag
      541 aggttgagaa ttcaaccggg ctgaccaaag caatggatga accttttgcg tacgactgct
      601 taccggaagt cggcgtatta tccagcgtaa tcatcttgcc agttaccaca tccagaatgc
      661 gcggcttctt tcgtttgtat gtaccagtat caaattgtgc atgctcctgc caggcttcga
      721 gctggccttt cgttgctccg ttcaagtcag cagtagctgc cataagttgc tcacgaacat
      781 actgtaaata ttgggtattc atgcagtaaa tcctttctat attttggcat aattcttcaa
      841 cattcggtaa tcgttcaaaa ccgaatcggg gaaacgacat aagcacagga gcccccagcg
      901 acagcgaagg agttctgata tataagactc agacatcatt cattccccgg ttctccaata
      961 tctgtttcac tcatcatcca taactacctg taat
//
LOCUS       DAZKHF020000591          994 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2201S31241
            SAMN43267800-rid25106413.denovo.595, whole genome shotgun sequence.
ACCESSION   DAZKHF020000591 DAZKHF020000000
VERSION     DAZKHF020000591.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267800
            Sequence Read Archive: SRR30307263
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 994)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 994)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 994)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350581.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350581.2
            Genome Coverage       :: 151x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:21:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,652
            CDSs (total)                      :: 5,557
            Genes (coding)                    :: 5,320
            CDSs (with protein)               :: 5,320
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 237
            CDSs (without protein)            :: 237
            Pseudo Genes (ambiguous residues) :: 0 of 237
            Pseudo Genes (frameshifted)       :: 102 of 237
            Pseudo Genes (incomplete)         :: 130 of 237
            Pseudo Genes (internal stop)      :: 52 of 237
            Pseudo Genes (multiple problems)  :: 41 of 237
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..994
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267800-rid25106413.denovo.595"
                     /serotype="O 26:H11"
                     /isolate="2201S31241"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-01"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            <1..509
                     /locus_tag="ACHOSC_RS26085"
                     /old_locus_tag="pgaptmp_005217"
     CDS             <1..509
                     /locus_tag="ACHOSC_RS26085"
                     /old_locus_tag="pgaptmp_005217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF1627 domain-containing protein"
                     /protein_id="HGU4550945.1"
                     /translation="EFIGQDGAKTCEELAGKFGVSTRKVASTLAVVTATGRLARVNQN
                     GKFRYCMPGDNLPAEPKAALVTESDGKVFPQPAGAALPVREAATQEEIKTESVAVTVQ
                     SQPSFTRKHPDGLILPSLHVANRELRRAKGQVQKWERVCAALRELNKHRDIVRQITDS
                     SRRVVSEK"
     gene            525..947
                     /locus_tag="ACHOSC_RS26090"
                     /old_locus_tag="pgaptmp_005218"
     CDS             525..947
                     /locus_tag="ACHOSC_RS26090"
                     /old_locus_tag="pgaptmp_005218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF977 family protein"
                     /protein_id="HGU4550946.1"
                     /translation="MAKVFTQEEREKIKGQVVELVRRSGRETLRQLEAKTGATRYLMS
                     VLARELVASGDVYNSGYGLFPSEQARKDWQNARKKLSRAKLKKPVVVDPDLIWSLPDG
                     EIRRYDRRLNIICSECRKSEAMQRVLAFYQGNFQKVLL"
BASE COUNT          266 a          216 c          301 g          211 t
ORIGIN      
        1 ttgagtttat cggtcaggat ggggctaaaa cgtgtgagga actggcgggt aagttcggtg
       61 tcagtactcg caaggttgct tccacgctgg cggtggtaac cgcaacgggg cggctggcac
      121 gcgttaatca gaacggtaaa tttcgttact gcatgccggg cgataattta ccagcagagc
      181 cgaaagccgc gctggtaacg gaaagtgatg gtaaggtctt tcctcagcca gcaggtgctg
      241 cgttaccagt ccgggaagcc gcaacacagg aagaaataaa aacggaaagt gtggcggtca
      301 cagtgcagtc acagccgtcg ttcaccagaa agcatccgga tggtctgatt ttaccatcgc
      361 tgcatgtggc taaccgcgag ctgcgccggg caaaaggtca ggttcagaag tgggagcgag
      421 tctgcgccgc gctgcgggag ctgaacaagc accgggatat tgttcgacag attactgatt
      481 cttcccgccg tgttgtatcg gaaaagtgat tgccggaggc gcttatggca aaagtattta
      541 cacaggaaga gcgggaaaaa attaaagggc aggttgttga gctagtacgc cggagtgggc
      601 gcgagacgtt acggcaactg gaagccaaga caggtgcgac aagatatctg atgagcgttc
      661 ttgccagaga gctggttgcc agtggcgatg tatacaactc tggttacggg ttattcccat
      721 ctgaacaggc tcgtaaggac tggcaaaacg cccgcaaaaa actatcgagg gcaaaactga
      781 agaaaccggt tgtggttgat cctgacctta tctggtcatt accagacgga gaaatacgcc
      841 gctacgacag gcgtctgaac ataatctgta gcgagtgccg gaagagcgaa gctatgcagc
      901 gtgtactggc tttctatcag ggtaattttc agaaggtgct gttgtgagcc aaattaacaa
      961 tcggaacttc gtgaagagaa agcataatcc aaat
//
LOCUS       DAZKHF020000592          992 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2201S31241
            SAMN43267800-rid25106413.denovo.596, whole genome shotgun sequence.
ACCESSION   DAZKHF020000592 DAZKHF020000000
VERSION     DAZKHF020000592.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267800
            Sequence Read Archive: SRR30307263
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 992)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 992)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 992)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350581.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350581.2
            Genome Coverage       :: 151x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:21:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,652
            CDSs (total)                      :: 5,557
            Genes (coding)                    :: 5,320
            CDSs (with protein)               :: 5,320
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 237
            CDSs (without protein)            :: 237
            Pseudo Genes (ambiguous residues) :: 0 of 237
            Pseudo Genes (frameshifted)       :: 102 of 237
            Pseudo Genes (incomplete)         :: 130 of 237
            Pseudo Genes (internal stop)      :: 52 of 237
            Pseudo Genes (multiple problems)  :: 41 of 237
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..992
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267800-rid25106413.denovo.596"
                     /serotype="O 26:H11"
                     /isolate="2201S31241"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-01"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            complement(<1..286)
                     /locus_tag="ACHOSC_RS26095"
                     /old_locus_tag="pgaptmp_005219"
     CDS             complement(<1..286)
                     /locus_tag="ACHOSC_RS26095"
                     /old_locus_tag="pgaptmp_005219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T3SS effector protein NleG8"
                     /protein_id="HGU4550947.1"
                     /translation="MPVTTLSIPSISQLSPAGVQSLQDAARLESGIRISIGSGQYSVH
                     YVQLLDGFSVEPVRGGLLDRLLGREHRMERRAVALERQLNGGVDFLSSVNN"
     gene            complement(354..935)
                     /locus_tag="ACHOSC_RS26100"
                     /old_locus_tag="pgaptmp_005220"
     CDS             complement(354..935)
                     /locus_tag="ACHOSC_RS26100"
                     /old_locus_tag="pgaptmp_005220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001118092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004842 - ubiquitin-protein transferase
                     activity [Evidence IEA];
                     GO_process: GO:0044403 - biological process involved in
                     symbiotic interaction [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="T3SS effector NleG family protein"
                     /protein_id="HGU4550948.1"
                     /translation="MPGLVSYISSTSFANEMAEMRQQVMEGQIGGFLLGGERVRVSYM
                     PDTGRFLAESEGLGLVYAELLNIGFNDGVDALRNRVLSVLPGMVAQRQENSLQAKISE
                     CTFTVDIEKLHCPGEVLQCPITLEQPEKGIFVKNSDGSDVCTLFDAAAFSRLTGEGLP
                     HPLTREPITASIIVKHEECIYDDTRGNFVIKGN"
BASE COUNT          292 a          254 c          158 g          288 t
ORIGIN      
        1 agttattaac actacttaaa aaatcgacac ctccatttaa ttgcctttcc agagccacag
       61 cccttctctc cattcgatgc tcacgcccca atagcctatc cagtaagcct cctctcaccg
      121 gttcaactga aaatccatcc agtagttgga catagtgaac agaatattgg ccactaccaa
      181 tggatattct tattccactt tcaagtctgg ctgcatcctg caaagactgc actcctgcag
      241 gagataattg agatatactt gggatactta aggtggtaac aggcataaaa acctcattca
      301 gtaattcata tcagtttgca ggatgtttca ttaaataaag ggtaatgttc atttcaatta
      361 ccctttataa cgaagtttcc tctggtatcg tcataaatgc attcttcatg ttttacaatt
      421 attgatgccg ttattggttc ccgggtcagt gggtggggta agccttcacc agtcaaacga
      481 gaaaatgcag cggcatcaaa taaagtacat acatctgaac catctgaatt cttcacaaaa
      541 ataccttttt caggctgctc cagtgtaatt ggacattgaa gcacctcacc aggacagtga
      601 agtttttcaa tatcaacagt aaaggtacat tccgatattt tggcctgcaa agagttctct
      661 tgtcgctgcg ccaccattcc aggaagcacg cttaacaccc tgtttctgag cgcatcaact
      721 ccatcattaa aaccaatatt caataattct gcataaacca gtcccagccc ttcactttct
      781 gctaaaaaac ggcctgtatc tggcatataa gaaactctaa ctctctcccc tcccaggaga
      841 aatcctccaa tctgcccttc cattacctgc tgacgcattt ccgccatctc attcgcgaat
      901 gaagtcgatg atatatatga tactaatcca ggcattacca tattccttca tacagataaa
      961 tatttcctct gggcttcata acaaacatct cc
//
LOCUS       DAZKHF020000593          988 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2201S31241
            SAMN43267800-rid25106413.denovo.597, whole genome shotgun sequence.
ACCESSION   DAZKHF020000593 DAZKHF020000000
VERSION     DAZKHF020000593.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267800
            Sequence Read Archive: SRR30307263
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 988)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 988)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 988)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350581.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350581.2
            Genome Coverage       :: 151x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:21:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,652
            CDSs (total)                      :: 5,557
            Genes (coding)                    :: 5,320
            CDSs (with protein)               :: 5,320
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 237
            CDSs (without protein)            :: 237
            Pseudo Genes (ambiguous residues) :: 0 of 237
            Pseudo Genes (frameshifted)       :: 102 of 237
            Pseudo Genes (incomplete)         :: 130 of 237
            Pseudo Genes (internal stop)      :: 52 of 237
            Pseudo Genes (multiple problems)  :: 41 of 237
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..988
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267800-rid25106413.denovo.597"
                     /serotype="O 26:H11"
                     /isolate="2201S31241"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-01"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            262..852
                     /locus_tag="ACHOSC_RS26105"
                     /old_locus_tag="pgaptmp_005221"
     CDS             262..852
                     /locus_tag="ACHOSC_RS26105"
                     /old_locus_tag="pgaptmp_005221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415892.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000150 - DNA strand exchange activity
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HGU4550949.1"
                     /translation="MSRIFAYCRISTLDQTIENQRREIESAGFKIKPQQIIEEHISGS
                     AATSERPGFNRLLARLKCGDQLIVTKLDRLGCNAMDIRKTVEQLTETGIRVHCLALGG
                     IDLTSPTGKMMMHVISAVAEFERDLLLERTHSGIVRARGAGKRFGRPPVLNEEQKQVV
                     FERIKSGVSISAIAREFKTSRQTILRAKAKLQTPDI"
BASE COUNT          315 a          211 c          203 g          259 t
ORIGIN      
        1 tttccacgac caaacaactt gaaatctggt taaaataaca cgcaacacta ttcttcttcc
       61 ttgagtccgc ccggaactcg aaaaacaaac cgagttaaag ccatttttca caaaatcgat
      121 tttgggtctc accaaaatta cggggttgca tacgcattcg tttattttcg aacgtgtaca
      181 tacaaatatg cacaaaaata atcaaaatta ttttctgaga tgcattatga tatgaacacc
      241 aatttcgtat agagtctcac tatgtctcga atttttgctt actgtcggat atcaacgctg
      301 gatcagacca tcgaaaatca acgccgggaa atcgaaagtg caggttttaa aatcaaacct
      361 cagcaaataa tcgaagaaca cattagcggc tcagcagcaa ccagtgagcg tcctggtttt
      421 aaccggttgc ttgctcgcct gaaatgtggt gatcaattga ttgtgacaaa actggatcgc
      481 cttggttgta atgcaatgga tatcaggaaa acagtggaac aactgaccga aacaggtatc
      541 agagtgcatt gcttagcatt ggggggcatt gacctgacca gtccaacagg aaaaatgatg
      601 atgcacgtaa tttcagcagt cgctgaattt gaacgagacc ttttacttga acgcactcat
      661 tccgggatag taagagcccg cggcgcaggg aaacgttttg gtcgaccacc tgtgttaaat
      721 gaagaacaga aacaggtggt attcgaacga attaagtcag gtgtaagtat aagtgccatt
      781 gcccgggaat tcaaaacctc gcggcaaacc attttaagag ccaaagcaaa acttcagaca
      841 cctgacatat aaaaaataat ctcggtgtga gatgctttac gtcttccaag cccccttcct
      901 tgccgtaaat ggaaagatac atctaattat agaatttata tgttttaccc tacggcagtg
      961 ctggccattc aatatcctgt gcagttga
//
LOCUS       DAZKHF020000594          984 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2201S31241
            SAMN43267800-rid25106413.denovo.598, whole genome shotgun sequence.
ACCESSION   DAZKHF020000594 DAZKHF020000000
VERSION     DAZKHF020000594.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267800
            Sequence Read Archive: SRR30307263
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 984)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 984)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 984)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350581.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350581.2
            Genome Coverage       :: 151x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:21:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,652
            CDSs (total)                      :: 5,557
            Genes (coding)                    :: 5,320
            CDSs (with protein)               :: 5,320
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 237
            CDSs (without protein)            :: 237
            Pseudo Genes (ambiguous residues) :: 0 of 237
            Pseudo Genes (frameshifted)       :: 102 of 237
            Pseudo Genes (incomplete)         :: 130 of 237
            Pseudo Genes (internal stop)      :: 52 of 237
            Pseudo Genes (multiple problems)  :: 41 of 237
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..984
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267800-rid25106413.denovo.598"
                     /serotype="O 26:H11"
                     /isolate="2201S31241"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-01"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            92..>984
                     /gene="tdcA"
                     /locus_tag="ACHOSC_RS26110"
                     /old_locus_tag="pgaptmp_005222"
     CDS             92..>984
                     /gene="tdcA"
                     /locus_tag="ACHOSC_RS26110"
                     /old_locus_tag="pgaptmp_005222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator TdcA"
                     /protein_id="HGU4550950.1"
                     /translation="MSTILLPKTQHLVVFQEVIRSGSIGSAAKELGLTQPAVSKIIND
                     IEDYFGVELVVRKNTGVTLTPAGQLLLSRSESITREMKNMVNEISGMSSEAVVEVSFG
                     FPSLIGFTFMSGMINKFKEVFPKAQVSMYEAQLSSFLPAIRDGRLDFAIGTLSAEMKL
                     QDLHVEPLFESEFVLVASKSRTCTGTTTLESLKNEQWVLPQTNMGYYSELLTTLQRNG
                     ISIENIVKTDSVVTIYNLVLNADFLTVIPCDMTSPFGSNQFITIPVEETLPVAQYAVV
                     WSKNYRIKKAASVLVELAKEYS"
BASE COUNT          270 a          189 c          226 g          299 t
ORIGIN      
        1 tgaaagttaa tttgtgagtg gtcgcacata tcctgttcat ttcattttga tacacttcat
       61 gccgtcaatg aggtaattaa cgtaggtcgt tatgagcact attcttcttc cgaaaacgca
      121 gcacctggta gtctttcagg aagtcattag aagtggctct atcggctcgg ctgcaaaaga
      181 attagggtta actcaaccgg ccgtcagtaa aatcattaac gatattgaag attattttgg
      241 tgtggaatta gtggtgcgga aaaataccgg tgtaacatta acacctgccg gtcaattgtt
      301 actctcccgt tccgaatcca ttacccgtga aatgaaaaat atggttaatg agataagcgg
      361 tatgtcttct gaggcggtgg tggaagtctc atttggtttt ccttcattga ttggttttac
      421 ttttatgtca gggatgatca acaagttcaa agaggtgttc ccgaaagcgc aggtttctat
      481 gtatgaagcg caactgtctt cgttcttacc ggcaatccgc gacggtcgac tggattttgc
      541 gattggtacg ttaagtgcag aaatgaagct tcaggattta catgttgagc cgctgttcga
      601 gtccgagttt gtgctggtag ccagtaagtc ccgaacatgc accggcacca ccacgctgga
      661 gtcgttgaag aacgaacagt gggtgttgcc acaaaccaat atggggtact acagcgagct
      721 tcttactacg ttacaaagaa atggcatcag tattgaaaac atcgttaaaa ccgactcagt
      781 cgtgacaatt tataatcttg ttctcaatgc tgatttctta actgtaattc cttgtgatat
      841 gacgtcacct tttggttcaa atcaatttat tactattccg gttgaagaaa cattacctgt
      901 ggcacaatat gccgtggtat ggtcgaaaaa ttatcgtatt aaaaaagcag catcggtttt
      961 ggtggaatta gccaaagagt attc
//
LOCUS       DAZKHF020000595          982 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2201S31241
            SAMN43267800-rid25106413.denovo.599, whole genome shotgun sequence.
ACCESSION   DAZKHF020000595 DAZKHF020000000
VERSION     DAZKHF020000595.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267800
            Sequence Read Archive: SRR30307263
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 982)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 982)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 982)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350581.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350581.2
            Genome Coverage       :: 151x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:21:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,652
            CDSs (total)                      :: 5,557
            Genes (coding)                    :: 5,320
            CDSs (with protein)               :: 5,320
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 237
            CDSs (without protein)            :: 237
            Pseudo Genes (ambiguous residues) :: 0 of 237
            Pseudo Genes (frameshifted)       :: 102 of 237
            Pseudo Genes (incomplete)         :: 130 of 237
            Pseudo Genes (internal stop)      :: 52 of 237
            Pseudo Genes (multiple problems)  :: 41 of 237
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..982
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267800-rid25106413.denovo.599"
                     /serotype="O 26:H11"
                     /isolate="2201S31241"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-01"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            complement(240..911)
                     /gene="queE"
                     /locus_tag="ACHOSC_RS26115"
                     /old_locus_tag="pgaptmp_005223"
     CDS             complement(240..911)
                     /gene="queE"
                     /locus_tag="ACHOSC_RS26115"
                     /old_locus_tag="pgaptmp_005223"
                     /EC_number="4.3.99.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001199960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="7-carboxy-7-deazaguanine synthase QueE"
                     /protein_id="HGU4550951.1"
                     /translation="MQYPINEMFQTLQGEGYFTGVPAIFIRLQGCPVGCAWCDTKHTW
                     EKLEDREVSLFSILAKTKESDKWGAASSEDLLAVIGRQGYTARHVVITGGEPCIHDLL
                     PLTDLLEKNGFSCQIETSGTHEVRCTPNTWVTVSPKLNMRGGYDVLSQALERANEIKH
                     PVGRVRDIEALDELLATLTDDKPRVIALQPISQKDDATRLCIETCIARNWRLSMQTHK
                     YLNIA"
BASE COUNT          271 a          242 c          205 g          264 t
ORIGIN      
        1 acttaatcca ttgattaaaa aggtaaatat ttaaaataac tctatacaaa ctaaaatcta
       61 ccaaacttta ccgcaataat tttcactcca gcgaaaaatt aatgccacag aatttgtaga
      121 gaatgctaat gattagccct ggatgggtaa aatggaggag ttttcagagg cgacaaacaa
      181 tataaatgag tagaagtctt tacaggttcc acccatttat aacgcttata aatgtttaat
      241 caggcaatat ttagatattt atgtgtttgc atcgacaaac gccaattacg cgcaatgcag
      301 gtttcaatgc acaaacgtgt ggcatcatcc ttttggctaa tcggctgcag tgcaatgact
      361 cgcggtttat catcggtcag cgttgccagt agttcatcca gtgcttcaat atcacgtacg
      421 cgccccactg gatgcttgat ttcgttggct cgctccagtg cctgtgacaa cacgtcatag
      481 ccgccgcgca tgttcagctt tggcgatacg gtaacccagg tattcggtgt gcagcgtact
      541 tcatgagttc cactggtttc gatctggcag ctaaaaccgt tcttttcgag caggtcagtc
      601 agtggcagca aatcatgaat gcaaggctca ccacccgtaa tcaccacatg ccgcgcggta
      661 tatccctgcc gaccaatgac agccagcaaa tcttcactgc tcgccgcccc ccacttatca
      721 ctctctttgg tcttcgccag aatgctgaaa agggagactt cccgatcctc aagcttttcc
      781 caggtgtgtt tggtgtcgca ccaggcacag ccaaccgggc atccctgtaa acgaataaaa
      841 atggcgggga cgccggtaaa gtaaccctca ccttgcaggg tctggaacat ctcgttaatc
      901 gggtactgca tagcattctc tgtgaagtgg ataattgtta attattgcag atcctgccac
      961 aacaatcatg tcttattaac at
//
LOCUS       DAZKHF020000596          976 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2201S31241
            SAMN43267800-rid25106413.denovo.600, whole genome shotgun sequence.
ACCESSION   DAZKHF020000596 DAZKHF020000000
VERSION     DAZKHF020000596.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267800
            Sequence Read Archive: SRR30307263
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 976)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 976)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 976)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350581.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350581.2
            Genome Coverage       :: 151x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:21:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,652
            CDSs (total)                      :: 5,557
            Genes (coding)                    :: 5,320
            CDSs (with protein)               :: 5,320
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 237
            CDSs (without protein)            :: 237
            Pseudo Genes (ambiguous residues) :: 0 of 237
            Pseudo Genes (frameshifted)       :: 102 of 237
            Pseudo Genes (incomplete)         :: 130 of 237
            Pseudo Genes (internal stop)      :: 52 of 237
            Pseudo Genes (multiple problems)  :: 41 of 237
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..976
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267800-rid25106413.denovo.600"
                     /serotype="O 26:H11"
                     /isolate="2201S31241"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-01"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            complement(61..348)
                     /locus_tag="ACHOSC_RS26120"
                     /old_locus_tag="pgaptmp_005224"
     CDS             complement(61..348)
                     /locus_tag="ACHOSC_RS26120"
                     /old_locus_tag="pgaptmp_005224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebO family protein"
                     /protein_id="HGU4550952.1"
                     /translation="MNEVVNSGVMNIASLVVSVVVLLIGLILWFFINRASSRTNEQIE
                     LLEALLDQQKRQNALLRRLCEANEPEKADKKTVESQKSVEDEDIIRLVAER"
     gene            complement(423..566)
                     /gene="mgrB"
                     /locus_tag="ACHOSC_RS26125"
                     /old_locus_tag="pgaptmp_005225"
     CDS             complement(423..566)
                     /gene="mgrB"
                     /locus_tag="ACHOSC_RS26125"
                     /old_locus_tag="pgaptmp_005225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PhoP/PhoQ regulator MgrB"
                     /protein_id="HGU4550953.1"
                     /translation="MKKFRWVVLVVVVLACLLLWAQVFNMMCDQDVQFFSGICAINQF
                     IPW"
     gene            725..964
                     /locus_tag="ACHOSC_RS26130"
                     /old_locus_tag="pgaptmp_005226"
     CDS             725..964
                     /locus_tag="ACHOSC_RS26130"
                     /old_locus_tag="pgaptmp_005226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YobH family protein"
                     /protein_id="HGU4550954.1"
                     /translation="MRFIIRTVMLIALVWIGLLLSGYGVLIGSKENAAGLGLQCTYLT
                     ARGTSTVQYLHTKSGFLGITDCPLLRKSNIVVDNG"
BASE COUNT          298 a          220 c          202 g          256 t
ORIGIN      
        1 tataatcagg atgaattgag taacccttca acgacggggt tactcaagat tgaggaagga
       61 ttatcgttcg gcgaccagac gaataatatc ttcgtcttca accgattttt gactctcaac
      121 ggtcttttta tctgctttct caggttcgtt tgcttcgcac aaacgacgta acagtgcatt
      181 ttgacgtttt tgctgatcca gcaacgcctc aagcagttca atctgttcgt tagtccgaga
      241 actggcacga ttgataaaaa accacaagat gagcccgata agaagaacca ccaccgatac
      301 aaccaaagac gcaatgttca tcacgcctga atttacaact tcgttcattt caccacctct
      361 gagtagaggc gctattctac cactgctgga gaggaagaaa atctagtgct gaaaaaatga
      421 tatcaccacg ggataaactg gttaatggca caaattccgc tgaaaaattg tacatcctga
      481 tcgcacatca tgttgaatac ctgcgcccaa agcagcaagc aagccaacac cacgacaacc
      541 agaacgaccc atcgaaactt tttcactcca atctccgctt cacctacctt atgtcattaa
      601 actagcatgc gttagttaaa cagcgcctaa ctatgtcgat attcgtgctt tttcggaatg
      661 agtagcttgc ttcagcgcat attgccgcta cgattaagcg aacataaaaa gagaagaggt
      721 tgtaatgcga ttcatcattc gcacagttat gctgatagca ctggtatgga taggattatt
      781 actcagcgga tatggcgttt tgattggaag caaagagaat gccgcaggat taggtttgca
      841 atgtacctat ctgactgcca gaggcaccag cacggtacaa tacttgcaca ctaaaagtgg
      901 tttcttgggg ataacagatt gcccactgtt acgtaaaagc aatatcgtgg tcgataatgg
      961 ttaagggtaa taaata
//
LOCUS       DAZKHF020000597          970 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2201S31241
            SAMN43267800-rid25106413.denovo.601, whole genome shotgun sequence.
ACCESSION   DAZKHF020000597 DAZKHF020000000
VERSION     DAZKHF020000597.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267800
            Sequence Read Archive: SRR30307263
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 970)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 970)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 970)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350581.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350581.2
            Genome Coverage       :: 151x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:21:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,652
            CDSs (total)                      :: 5,557
            Genes (coding)                    :: 5,320
            CDSs (with protein)               :: 5,320
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 237
            CDSs (without protein)            :: 237
            Pseudo Genes (ambiguous residues) :: 0 of 237
            Pseudo Genes (frameshifted)       :: 102 of 237
            Pseudo Genes (incomplete)         :: 130 of 237
            Pseudo Genes (internal stop)      :: 52 of 237
            Pseudo Genes (multiple problems)  :: 41 of 237
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..970
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267800-rid25106413.denovo.601"
                     /serotype="O 26:H11"
                     /isolate="2201S31241"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-01"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            complement(1..848)
                     /locus_tag="ACHOSC_RS26135"
                     /old_locus_tag="pgaptmp_005227"
                     /pseudo
     CDS             complement(1..848)
                     /locus_tag="ACHOSC_RS26135"
                     /old_locus_tag="pgaptmp_005227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310773.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1737 domain-containing protein"
     gene            825..970
                     /locus_tag="ACHOSC_RS26140"
                     /old_locus_tag="pgaptmp_005228"
                     /pseudo
     CDS             825..970
                     /locus_tag="ACHOSC_RS26140"
                     /old_locus_tag="pgaptmp_005228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_303550.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT          222 a          290 c          254 g          204 t
ORIGIN      
        1 ccacggcaca tctgcagcac tgccaccatg gcactgcgcg ttaaacacgg agaggtcagc
       61 acgaaactgt gtcagcatgg ctgtaaacag cgcaggttgc tgtacgtggg tggcggcgct
      121 catgtcaaac tcaccctgca tccagcagac ggccagcaga acgtttttgg gatttttctg
      181 caatgccgct tttgtgcggg aaatcagatc ctgatataac ggcttgccca ccccccagcg
      241 tgccgaatcc tgactggccc ccgtggactc gctgaatgtc ccctccgcgc cctgggtaaa
      301 tgccgaacca ccacgacagc atggtaccag caggatcccc gcgttattcg ggatatacgg
      361 gagcagtttt ttggcaatat gtaaaccctg gccgacacag ccgtactgcc ctttgctcag
      421 gtcagccctc ggatgattca gcgtactcat atcctgcaca tcatgcagac agtggtcagc
      481 cggaataata tcgttatatc tgcaggcagc cccgcccggc gtcactgtac tgcggcgcgc
      541 cagctgttta atgcgcggat ccggagcatc gtatgaatcc ggcagcggaa gcccttcacc
      601 gtaagccctt caccgtaagc catggcattg gattgcccgg ccagtacgat gacgtagtac
      661 caatccggct cagatgaagg gccgacctgt ggctctcctt caatagccac cgcctgcatc
      721 agtgtgtacg gcgtaatggc aaccggtccg ccgtatggct gccagccctc tttcagtttg
      781 tgtgtcagct tttccgcaag gtctgacggc gacgccgccc tgacaacatc gtaatgttta
      841 atcgacatcg aatttctccc gtgtagagga acagagttaa aaagccggaa gcggaatcaa
      901 atcacaggat gaccatctgc cagtggcagg tcataaaaaa aaggctgcgc aatgcgcagc
      961 cagaactcac
//
LOCUS       DAZKHF020000598          961 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2201S31241
            SAMN43267800-rid25106413.denovo.602, whole genome shotgun sequence.
ACCESSION   DAZKHF020000598 DAZKHF020000000
VERSION     DAZKHF020000598.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267800
            Sequence Read Archive: SRR30307263
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 961)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 961)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 961)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350581.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350581.2
            Genome Coverage       :: 151x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:21:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,652
            CDSs (total)                      :: 5,557
            Genes (coding)                    :: 5,320
            CDSs (with protein)               :: 5,320
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 237
            CDSs (without protein)            :: 237
            Pseudo Genes (ambiguous residues) :: 0 of 237
            Pseudo Genes (frameshifted)       :: 102 of 237
            Pseudo Genes (incomplete)         :: 130 of 237
            Pseudo Genes (internal stop)      :: 52 of 237
            Pseudo Genes (multiple problems)  :: 41 of 237
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..961
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267800-rid25106413.denovo.602"
                     /serotype="O 26:H11"
                     /isolate="2201S31241"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-01"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            <1..225
                     /locus_tag="ACHOSC_RS26145"
                     /old_locus_tag="pgaptmp_005229"
     CDS             <1..225
                     /locus_tag="ACHOSC_RS26145"
                     /old_locus_tag="pgaptmp_005229"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4550955.1"
                     /translation="FRGLPQPEAHGRRKFNDRLSMNQLPRRALSRYGLTFQGNILSVN
                     CQCRMLTRVRRTHSTSPVENSLITNLSFVG"
     gene            complement(229..447)
                     /gene="ydfC"
                     /locus_tag="ACHOSC_RS26150"
                     /old_locus_tag="pgaptmp_005230"
     CDS             complement(229..447)
                     /gene="ydfC"
                     /locus_tag="ACHOSC_RS26150"
                     /old_locus_tag="pgaptmp_005230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310310.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YdfC"
                     /protein_id="HGU4550956.1"
                     /translation="MQKREPVIIAPDYTDDELYEWMHQKIKAAQDLKWANEARAKQAE
                     NLSALEQDITNLEKAAALSIARMITYPR"
     gene            complement(519..818)
                     /locus_tag="ACHOSC_RS26155"
                     /old_locus_tag="pgaptmp_005231"
     CDS             complement(519..818)
                     /locus_tag="ACHOSC_RS26155"
                     /old_locus_tag="pgaptmp_005231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4550957.1"
                     /translation="MALYIDISAIAGQVRIIRAVTKRYAPLLQKVSGECTEDIVNDFV
                     IKLRGLIFSYKVTTIFADGSRETVRAMRFKGCVKDFATTFWARKLDCIHNQFPLE"
BASE COUNT          209 a          250 c          222 g          280 t
ORIGIN      
        1 tttcgcggat tgccacaacc ggaagcgcac ggtcgacgaa aatttaacga caggctatct
       61 atgaaccagc tacctcgccg tgcgctttcg cgttatggtc tgacttttca gggaaatatc
      121 ctttcagtaa actgtcagtg ccggatgctc acccgtgtcc ggcgcacgca ctccacctca
      181 cccgtggaga actccttaat taccaacctt agcttcgttg gttagctatt aacgcgggta
      241 tgtaatcatt ctggcaatgc ttaatgccgc tgctttttcc agattggtga tatcctgctc
      301 cagagcggac agattttcag cctgcttagc cctggcttca ttggcccatt tcaggtcctg
      361 cgctgcctta attttctggt gcatccactc ataaagttca tcatcggtat agtctggcgc
      421 gatgatgacg ggttctcgtt tctgcatgtc ggctccttgt ggttagcgct gcctgctttt
      481 aaccacgtca ggcgaggtgg tatcctctga ggggtctgtt actcgagagg aaattggtta
      541 tgaatacaat caagttttct tgcccagaat gtggtggcga agtctttgac acatccttta
      601 aaccgcatgg ctctgacagt ttcgcgggag ccatctgcaa aaattgtggt caccttgtaa
      661 ctgaagatga gtcctcgcag tttgatgacg aaatcgttga caatatcttc ggtgcactca
      721 ccagagactt tctgaagtaa aggcgcatac cgcttagtta ccgctctgat aattcttacc
      781 tgtccggcaa tggcgctgat atcaatataa agcgccatcg ctgtttcttt gctgatccct
      841 ggatgccttc cattctgatg tttgacttcg cccactgaga aatcctctgt ttccccttaa
      901 cgccggggta gcggaacaaa aaacctgctg catagttaaa cgtaatcccc gccgtcatgt
      961 t
//
LOCUS       DAZKHF020000599          959 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2201S31241
            SAMN43267800-rid25106413.denovo.603, whole genome shotgun sequence.
ACCESSION   DAZKHF020000599 DAZKHF020000000
VERSION     DAZKHF020000599.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267800
            Sequence Read Archive: SRR30307263
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 959)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 959)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 959)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350581.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350581.2
            Genome Coverage       :: 151x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:21:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,652
            CDSs (total)                      :: 5,557
            Genes (coding)                    :: 5,320
            CDSs (with protein)               :: 5,320
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 237
            CDSs (without protein)            :: 237
            Pseudo Genes (ambiguous residues) :: 0 of 237
            Pseudo Genes (frameshifted)       :: 102 of 237
            Pseudo Genes (incomplete)         :: 130 of 237
            Pseudo Genes (internal stop)      :: 52 of 237
            Pseudo Genes (multiple problems)  :: 41 of 237
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..959
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267800-rid25106413.denovo.603"
                     /serotype="O 26:H11"
                     /isolate="2201S31241"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-01"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            <1..882
                     /locus_tag="ACHOSC_RS26160"
                     /old_locus_tag="pgaptmp_005232"
     CDS             <1..882
                     /locus_tag="ACHOSC_RS26160"
                     /old_locus_tag="pgaptmp_005232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HGU4550958.1"
                     /translation="FPFIGNRDITTLNTPDLLIPVRAAEAKQIYEIASRLQQRISAVM
                     RYAVQSGIIRYNPALDMAGALTTVKRQHRPALDLSRLPELLSRIGSYKGQPVTQLAVM
                     LNLLVFIRSSELRYARWSEIDIDNAMWTIPAEREPLPGVKFSHRGSKMRTPHLVPLSK
                     QVVAILAELQTWAGENGLIFTGAHDPRKPISENTVNKALRVMGYDTTQDVCGHGFRAM
                     ACSALIESGLWSRDAVERQMSHQERNGVRAAYIHKAEHLEERRLMLQWWADFLDANRE
                     RFISPFEYAKINNPLKQ"
BASE COUNT          267 a          229 c          219 g          244 t
ORIGIN      
        1 ttccccttta tcggcaatcg ggatatcaca acactcaata ccccggacct gcttatccct
       61 gttcgtgctg cagaagctaa acaaatttat gaaatcgcca gtcgtctgca gcaaagaata
      121 tctgccgtaa tgcgttatgc cgtacagtct ggcatcatca gatataatcc tgctctggat
      181 atggctggcg cattgactac ggtaaaacgc cagcatcgcc ccgctcttga tctttctcgc
      241 ctgcctgaac ttttgtcgcg tattggcagt tacaaggggc aacctgtcac ccagcttgcc
      301 gttatgctga atttactggt ttttattcgt tccagtgaac tcagatacgc ccgctggtct
      361 gaaattgata ttgacaatgc catgtggact attccagccg aacgcgaacc tctgccaggc
      421 gtaaaattct cacaccgggg atccaagatg cgaacaccac atcttgtgcc actcagcaaa
      481 caggttgtag ccatactggc agaacttcag acatgggctg gtgaaaatgg tctgatcttt
      541 acgggagcac atgacccacg taaaccaatc agtgaaaaca ccgtaaataa agccctgagg
      601 gttatggggt atgacacaac ccaggatgtc tgtggtcatg gattccgggc gatggcatgt
      661 agtgcgttaa tagaatcagg tttgtggtcc cgcgatgcag ttgaacgtca gatgagccat
      721 caggaacgta acggtgtacg tgctgcttac attcataaag cagaacatct ggaagaacgt
      781 cgactgatgc tacagtggtg ggccgatttt ctggatgcaa acagagaaag atttatcagt
      841 ccatttgaat atgcaaagat taataatcca ttaaaacagt aatcatcccg ggcaaatgcc
      901 cgggaaccta ctccaggatt attttctttg ttaaaaaaac agacaaaacg gtattaact
//
LOCUS       DAZKHF020000600          957 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2201S31241
            SAMN43267800-rid25106413.denovo.604, whole genome shotgun sequence.
ACCESSION   DAZKHF020000600 DAZKHF020000000
VERSION     DAZKHF020000600.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267800
            Sequence Read Archive: SRR30307263
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 957)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 957)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 957)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350581.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350581.2
            Genome Coverage       :: 151x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:21:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,652
            CDSs (total)                      :: 5,557
            Genes (coding)                    :: 5,320
            CDSs (with protein)               :: 5,320
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 237
            CDSs (without protein)            :: 237
            Pseudo Genes (ambiguous residues) :: 0 of 237
            Pseudo Genes (frameshifted)       :: 102 of 237
            Pseudo Genes (incomplete)         :: 130 of 237
            Pseudo Genes (internal stop)      :: 52 of 237
            Pseudo Genes (multiple problems)  :: 41 of 237
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..957
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267800-rid25106413.denovo.604"
                     /serotype="O 26:H11"
                     /isolate="2201S31241"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-01"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            complement(72..956)
                     /gene="xapR"
                     /locus_tag="ACHOSC_RS26165"
                     /old_locus_tag="pgaptmp_005233"
     CDS             complement(72..956)
                     /gene="xapR"
                     /locus_tag="ACHOSC_RS26165"
                     /old_locus_tag="pgaptmp_005233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator XapR"
                     /protein_id="HGU4550959.1"
                     /translation="MERVYRTDLKLLRYFLAVAEELHFGRAAARLNMSQPPLSIHIKE
                     LENQLGTQLFIRHSRSVVLTHAGKILMEESRRLLVNANNVLARVEQIGRGEAGRIELG
                     VVGTAMWGRMRPVMRRFLRENPNVEVLFREKMPAMQMALLERRELDAGIWRMATEPPT
                     GFTSLRLHESAFLVAMPEEHHLSSFSTVPLEALRDEYFVTMPPVYTDWDFLQRVCQQV
                     GFSPVVIREVNEPQTVLAMVSMGIGITLIADSYAQMNWPGVIFRPLKQRIPADLYIVY
                     ETQQVTPAMVKLLAALTQ"
BASE COUNT          242 a          265 c          217 g          233 t
ORIGIN      
        1 tgcgatttgc gaaattgcct gatgcgcttc acttagcaga cgactatttc cggcaattcc
       61 tgtctcctca cctactgtgt caatgcagcc aacagcttaa ccatcgcggg cgtcacctgc
      121 tgtgtttcat aaacaatata taaatctgca gggatgcgct gtttgagcgg acggaaaatg
      181 acacctggcc agttcatttg tgcgtagctg tccgctatca atgtgatacc aatacccata
      241 ctgaccatgg cgagtaccgt ttgcggttca ttaacttcgc gaataacaac cggtgaaaat
      301 cccacctgct ggcaaactcg ctgcaaaaaa tcccagtcag tgtacacggg cggcattgta
      361 acaaaatact cgtcacgtag cgcttccagc ggaacggtgg aaaatgatga gagatgatgc
      421 tcttcaggca tcgccaccag aaacgccgat tcatgcaacc gtaagctggt aaaacctgtc
      481 ggtggttctg tcgccattcg ccagatcccg gcatcgagtt cgcgtcgttc cagcagggcc
      541 atttgcatcg cgggcatctt ttcgcgaaaa agaacttcaa cgttaggatt ttccctgagg
      601 aatcgccgca taaccgggcg catccgtccc cacatagccg ttcccactac gccgagttca
      661 atccgccctg cttctccccg acctatttgt tcaacccgag ccaatacatt attagcattc
      721 accagcaatc gacgcgattc ttccatcaag attttgcccg cgtgtgtcag tacgacgctg
      781 cgcgaatggc gaataaaaag ctgcgtgccg agttgatttt ccagctcttt aatatgaata
      841 ctgagcggag gctgagacat atttaaacgc gctgctgcgc ggccaaaatg caactcttcc
      901 gctacggcaa gaaaataacg gagcaactta agatctgttc tgtatacgcg ttccata
//
LOCUS       DAZKHF020000601          953 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2201S31241
            SAMN43267800-rid25106413.denovo.605, whole genome shotgun sequence.
ACCESSION   DAZKHF020000601 DAZKHF020000000
VERSION     DAZKHF020000601.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267800
            Sequence Read Archive: SRR30307263
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 953)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 953)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 953)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350581.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350581.2
            Genome Coverage       :: 151x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:21:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,652
            CDSs (total)                      :: 5,557
            Genes (coding)                    :: 5,320
            CDSs (with protein)               :: 5,320
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 237
            CDSs (without protein)            :: 237
            Pseudo Genes (ambiguous residues) :: 0 of 237
            Pseudo Genes (frameshifted)       :: 102 of 237
            Pseudo Genes (incomplete)         :: 130 of 237
            Pseudo Genes (internal stop)      :: 52 of 237
            Pseudo Genes (multiple problems)  :: 41 of 237
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..953
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267800-rid25106413.denovo.605"
                     /serotype="O 26:H11"
                     /isolate="2201S31241"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-01"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            37..>953
                     /locus_tag="ACHOSC_RS26170"
                     /old_locus_tag="pgaptmp_005234"
     CDS             37..>953
                     /locus_tag="ACHOSC_RS26170"
                     /old_locus_tag="pgaptmp_005234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid A deacylase LpxR family protein"
                     /protein_id="HGU4550960.1"
                     /translation="MKKSVIAGVFIALSFTTCSAIANSLALSLANDDAGKFQPILNDI
                     YGNKHENRDDYSQGLFLGYSHDISDSSQLSLHIAQDIYSPSGSNKRHNTAVTGDRAFS
                     AYTHTGIEWNSLANDWIRYRLGTDIGVVGPDAGGQKVQNKAHEIIGAEKYHAWDDQIE
                     NRYGYTVKGMLSMTPSMDILGANVGLYPEVSAVTGNLFQYVAYGATIAIGNDKTFNSD
                     NGFGLLAPRGLMHMSDTSGFKYKIFAGMERRDVNRNYTLEGKTIQTKQTTVSLNKTVD
                     EYQVGATIGYAPVAFTLAFNKVTSEFKTG"
BASE COUNT          320 a          184 c          196 g          253 t
ORIGIN      
        1 acgcgcacca ttcaaaaaaa aggaagacaa taacatatga aaaaaagtgt catcgctggc
       61 gtctttattg ctctgtcatt taccacgtgt tcagctatcg cgaacagcct tgcattatca
      121 ttagcaaatg atgatgcagg gaagtttcaa ccaatactta atgatattta tggcaataaa
      181 catgaaaaca gagatgatta ctcacaaggc ttatttctgg gatatagcca cgatatctca
      241 gactcgagcc aattatctct ccatattgcg caagatattt actctccatc aggcagtaat
      301 aaaagacaca acacagctgt aactggagac agagctttta gtgcatacac tcacactggt
      361 attgaatgga actcccttgc gaatgactgg attcgctatc gattaggtac tgacataggt
      421 gttgttggcc ccgacgcagg cggtcagaaa gtacaaaata aagctcatga gattattggg
      481 gcagaaaaat atcatgcatg ggatgatcaa atagagaatc gctacggtta tactgtaaaa
      541 gggatgctat ccatgacacc aagtatggat attttaggtg ctaatgttgg attataccct
      601 gaagtttctg ctgttactgg aaacttattt caatatgtag catatggcgc aaccattgcc
      661 attggtaatg ataaaacctt caattcggat aatggctttg gtctgctggc tccccgtggt
      721 ttaatgcata tgtccgatac aagcggattc aaatacaaga tttttgcagg tatggaaaga
      781 cgagatgtca atcgcaacta tactctcgaa ggaaaaacaa tacagacgaa acaaacaaca
      841 gtatcgctaa acaaaactgt tgatgaatat caagttggcg caacaattgg gtatgcacct
      901 gtagccttca cactagcatt taataaagta acatcagaat tcaagacagg gga
//
LOCUS       DAZKHF020000602          952 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2201S31241
            SAMN43267800-rid25106413.denovo.606, whole genome shotgun sequence.
ACCESSION   DAZKHF020000602 DAZKHF020000000
VERSION     DAZKHF020000602.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267800
            Sequence Read Archive: SRR30307263
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 952)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 952)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 952)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350581.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350581.2
            Genome Coverage       :: 151x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:21:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,652
            CDSs (total)                      :: 5,557
            Genes (coding)                    :: 5,320
            CDSs (with protein)               :: 5,320
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 237
            CDSs (without protein)            :: 237
            Pseudo Genes (ambiguous residues) :: 0 of 237
            Pseudo Genes (frameshifted)       :: 102 of 237
            Pseudo Genes (incomplete)         :: 130 of 237
            Pseudo Genes (internal stop)      :: 52 of 237
            Pseudo Genes (multiple problems)  :: 41 of 237
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..952
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267800-rid25106413.denovo.606"
                     /serotype="O 26:H11"
                     /isolate="2201S31241"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-01"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            11..>952
                     /locus_tag="ACHOSC_RS26175"
                     /old_locus_tag="pgaptmp_005235"
     CDS             11..>952
                     /locus_tag="ACHOSC_RS26175"
                     /old_locus_tag="pgaptmp_005235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416774.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M28 family metallopeptidase"
                     /protein_id="HGU4550961.1"
                     /translation="MKKINFAFIILFLFSLPLIIFYQPWVNALPPMPRHANPEQLEKT
                     VRYLTQTVHPRSADNIDNLNRSAEYIKEVFVSSGARITSQDVPITGGPYKNIVADYGP
                     ADGPLIIIGAHYDSASSYENDELTYTPGADDNASGVAGLLELARLLHQQVPKTGVQLV
                     AYASEEPPFFRSDEMGSAVHAASLERPVKLMIALEMIGYYDSAPGSQDYPYPAMSWLY
                     PDRGDFIAVVGRMQDINAVRQVKATLLSSRYLSVYSMNAPGFIPGIDFSDHLNYWQHD
                     IPAVMITDTAFYRNKQYHLPGDTADRLNYQKMAQVVDG"
BASE COUNT          250 a          221 c          214 g          267 t
ORIGIN      
        1 taatttgcat atgaaaaaaa taaattttgc ttttattata ttatttctgt tttcactccc
       61 tcttattatt ttttaccaac cctgggtcaa tgcattaccg ccaatgcccc gacacgcaaa
      121 tcctgagcaa ttagaaaaaa cagtgcgtta tcttacacaa actgtgcatc cacgtagtgc
      181 cgacaatatt gataatctga ataggtccgc agagtatata aaagaggtct tcgtcagtag
      241 cggtgccaga attacctcgc aggacgtccc cattacgggt ggcccctaca aaaacattgt
      301 tgctgattat ggtcctgccg atggaccgct gattattatt ggtgcgcact atgactctgc
      361 cagcagttat gaaaacgatg aattgaccta tacacctggc gcggatgata acgccagcgg
      421 cgtggcagga ttactcgaac tggcacgttt gttacatcag caagtaccga aaacaggcgt
      481 gcagctggtc gcctatgcgt cggaagaacc gcccttcttt cgtagcgatg aaatggggag
      541 cgcggtacat gcagcttcgc ttgagcgtcc agtaaaatta atgatagcac tggagatgat
      601 tggctattac gactctgcgc ctggtagcca ggattaccct tacccggcaa tgtcctggct
      661 ttatcccgat cggggagact ttattgccgt ggtcggcaga atgcaggata tcaacgccgt
      721 tcgtcaggta aaagcgacat tgttatcatc ccggtattta tctgtttatt ctatgaatgc
      781 cccagggttt attcccggta ttgatttctc tgatcacctt aattattggc aacacgatat
      841 tcccgccgta atgattactg acaccgcttt ttatcgtaat aaacaatacc acttgcccgg
      901 tgataccgca gacagattga attatcagaa aatggctcag gtggtggatg gt
//
LOCUS       DAZKHF020000603          951 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2201S31241
            SAMN43267800-rid25106413.denovo.607, whole genome shotgun sequence.
ACCESSION   DAZKHF020000603 DAZKHF020000000
VERSION     DAZKHF020000603.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267800
            Sequence Read Archive: SRR30307263
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 951)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 951)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 951)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350581.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350581.2
            Genome Coverage       :: 151x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:21:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,652
            CDSs (total)                      :: 5,557
            Genes (coding)                    :: 5,320
            CDSs (with protein)               :: 5,320
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 237
            CDSs (without protein)            :: 237
            Pseudo Genes (ambiguous residues) :: 0 of 237
            Pseudo Genes (frameshifted)       :: 102 of 237
            Pseudo Genes (incomplete)         :: 130 of 237
            Pseudo Genes (internal stop)      :: 52 of 237
            Pseudo Genes (multiple problems)  :: 41 of 237
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..951
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267800-rid25106413.denovo.607"
                     /serotype="O 26:H11"
                     /isolate="2201S31241"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-01"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            complement(<1..211)
                     /locus_tag="ACHOSC_RS26180"
                     /old_locus_tag="pgaptmp_005236"
     CDS             complement(<1..211)
                     /locus_tag="ACHOSC_RS26180"
                     /old_locus_tag="pgaptmp_005236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4550962.1"
                     /translation="MSTGLRFTLEVDGLPPDAFAVVSFHLNQSLSSLFSLDLSLVSQQ
                     FLSLEFAQVLDKMAYLTIWQGDEVQR"
BASE COUNT          309 a          197 c          192 g          253 t
ORIGIN      
        1 ggcgctgtac ttcatcgccc tgccatatcg tcaggtaggc cattttgtcc agcacctgcg
       61 caaattcaag ggagagaaac tgctggctga ccagggagag atcgagggaa aagagcgaag
      121 agagtgactg gttcagatga aaggaaacca ccgcaaaagc atccggcggc aggccgtcca
      181 cttccagtgt gaaacgtaat ccggttgaca tagcaacctc cgtgaaaatg aataataatc
      241 cgcacatatt gcatgtacaa catgtcgggc ggatgagata agcgagtcga catctggcgt
      301 taccggggaa tatcagaaaa atgtcatggt tatgtgatga tttagtctgg gattttactt
      361 cagagaaacg ggtgtctgtt tagtcaacaa aataatcaca aacgcaacgg atgggttctc
      421 gacgttattc acgacaggct cgtacattac agcgagtaat aaaaaacctg atatgcaaag
      481 tcaaaccaat attcataatg tctttttatt cacaacaata aataattcaa taacaatcat
      541 tgaaatgaat gatggtttgt ataaggaata tacgctaccg ccccaccctg ataacatgat
      601 ctgcacattg ccatcttaac gtcgttaaac actagcttaa tagtggatat ttcaaattag
      661 gaatttatta aattcacctg atgaatataa taagcaaata gcatagaaaa taatgacaac
      721 tcccccgagg ggaccatttc caaagcactg caccaccacc tggtgccgga tgatcttgct
      781 tcagtggtaa gagtacctga tgcattgttc ccaaaatggt gttttcccct gcccctccaa
      841 taatgtgtag ctctcacttg ataatctatt gattaggtta tattaagaat caacatttaa
      901 aatacatcca ttttcttgaa taaatcaaca tgtttattaa tcaattgctg t
//
LOCUS       DAZKHF020000604          949 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2201S31241
            SAMN43267800-rid25106413.denovo.608, whole genome shotgun sequence.
ACCESSION   DAZKHF020000604 DAZKHF020000000
VERSION     DAZKHF020000604.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267800
            Sequence Read Archive: SRR30307263
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 949)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 949)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 949)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350581.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350581.2
            Genome Coverage       :: 151x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:21:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,652
            CDSs (total)                      :: 5,557
            Genes (coding)                    :: 5,320
            CDSs (with protein)               :: 5,320
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 237
            CDSs (without protein)            :: 237
            Pseudo Genes (ambiguous residues) :: 0 of 237
            Pseudo Genes (frameshifted)       :: 102 of 237
            Pseudo Genes (incomplete)         :: 130 of 237
            Pseudo Genes (internal stop)      :: 52 of 237
            Pseudo Genes (multiple problems)  :: 41 of 237
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..949
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267800-rid25106413.denovo.608"
                     /serotype="O 26:H11"
                     /isolate="2201S31241"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-01"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            <1..789
                     /gene="yeeO"
                     /locus_tag="ACHOSC_RS26185"
                     /old_locus_tag="pgaptmp_005237"
     CDS             <1..789
                     /gene="yeeO"
                     /locus_tag="ACHOSC_RS26185"
                     /old_locus_tag="pgaptmp_005237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001323172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxic metabolite efflux MATE transporter YeeO"
                     /protein_id="HGU4550963.1"
                     /translation="RYIGAVAILWVLAIGFNPALRISLKSYFKPLNFSIIWEVMGIGI
                     PASVESVLFTSGRLLTQMFVAGMGTSVIAGNFIAFSIAALINLPGSALGSASTIITGR
                     RLGVGQIAQAEIQLRHVFWLSTLGLTAIAWLTAPFAGVMASFYTQDPQVKHVVVILIW
                     LNALFMPIWSASWVLPAGFKGARDARYAMWVSMLSMWGCRVVVGYVLGIMLGWGVVGV
                     WMGMFADWAVRAVLFYWRMVTGRWLWKYPRPEPQKCEKKPVVSE"
BASE COUNT          196 a          192 c          269 g          292 t
ORIGIN      
        1 cgttatattg gcgcagttgc aattttgtgg gtgctggcga ttggttttaa tcctgcgcta
       61 aggatttcgt taaagagcta ttttaaaccg ctgaatttta gcattatctg ggaagtcatg
      121 gggattggta ttccagcgag tgtcgaatca gtgttattta ccagtggtcg gttattaacc
      181 caaatgttcg ttgccgggat ggggaccagt gttattgccg gaaattttat cgcgttttca
      241 attgcggctc ttatcaactt acccggaagt gcgctcggct ctgcttctac gatcattaca
      301 ggccgaaggt taggggtagg gcagatagcg caagcagaga ttcagttgcg gcatgtcttc
      361 tggctgtcca ctcttggatt aacggccatc gcctggctaa cggctccctt tgccggggtt
      421 atggcatcgt tttacaccca ggatccacag gttaaacatg tcgttgtgat tctgatttgg
      481 ctaaatgctt tatttatgcc tatttggtcc gcctcatggg tgctacccgc tggatttaag
      541 ggggctcgtg acgcccgtta cgctatgtgg gtttctatgt tgagcatgtg gggttgccgg
      601 gttgtcgtcg gttatgtgct gggcatcatg cttggctggg gtgtggttgg tgtctggatg
      661 ggaatgtttg ccgactgggc tgtgcgggcc gtgctgtttt actggcgaat ggttactgga
      721 cgttggctat ggaaataccc tcgacccgaa ccgcaaaagt gtgaaaaaaa gccagttgtg
      781 tcggaataaa caacaaaatg cagattattt cagcaaacga tttcaaattt aaaaaacagg
      841 ctttgacatt gtgggtgggc atcgctaata ttcgcctcgt tctcacgatt cctctgtagt
      901 tcagtcggta gaacggcgga ctgttaatcc gtatgtcact ggttcgagt
//
LOCUS       DAZKHF020000605          934 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2201S31241
            SAMN43267800-rid25106413.denovo.609, whole genome shotgun sequence.
ACCESSION   DAZKHF020000605 DAZKHF020000000
VERSION     DAZKHF020000605.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267800
            Sequence Read Archive: SRR30307263
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 934)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 934)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 934)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350581.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350581.2
            Genome Coverage       :: 151x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:21:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,652
            CDSs (total)                      :: 5,557
            Genes (coding)                    :: 5,320
            CDSs (with protein)               :: 5,320
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 237
            CDSs (without protein)            :: 237
            Pseudo Genes (ambiguous residues) :: 0 of 237
            Pseudo Genes (frameshifted)       :: 102 of 237
            Pseudo Genes (incomplete)         :: 130 of 237
            Pseudo Genes (internal stop)      :: 52 of 237
            Pseudo Genes (multiple problems)  :: 41 of 237
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..934
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267800-rid25106413.denovo.609"
                     /serotype="O 26:H11"
                     /isolate="2201S31241"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-01"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            <1..223
                     /locus_tag="ACHOSC_RS26190"
                     /old_locus_tag="pgaptmp_005238"
     CDS             <1..223
                     /locus_tag="ACHOSC_RS26190"
                     /old_locus_tag="pgaptmp_005238"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4550964.1"
                     /translation="QRYDDSGVICTGGPGTLSGIATTKNSKSKGNGAGGVGGNRLIFR
                     YYRPVSAEGGSYLSGLRIPLDSITDWRNT"
     gene            complement(336..532)
                     /locus_tag="ACHOSC_RS26195"
                     /old_locus_tag="pgaptmp_005239"
                     /pseudo
     CDS             complement(336..532)
                     /locus_tag="ACHOSC_RS26195"
                     /old_locus_tag="pgaptmp_005239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001547002.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            708..>934
                     /locus_tag="ACHOSC_RS26200"
                     /old_locus_tag="pgaptmp_005240"
     CDS             708..>934
                     /locus_tag="ACHOSC_RS26200"
                     /old_locus_tag="pgaptmp_005240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000221568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inovirus-type Gp2 protein"
                     /protein_id="HGU4550965.1"
                     /translation="MTYKYNPFWQQRIRETVRHALNVHPRLTALRVDLRFPDVPAATD
                     AAVISRFINALKARIDAYQKRKHREGKRVHPT"
BASE COUNT          246 a          249 c          234 g          205 t
ORIGIN      
        1 gcagcgctac gatgactccg gtgttatctg caccggagga ccgggaacat taagcggtat
       61 agcgacaaca aagaattcga aatcaaaagg gaatggtgcc ggaggcgtgg gcggtaatag
      121 gctgattttc aggtattacc ggcctgtatc tgctgaaggg ggctcgtatt tgtcggggct
      181 gcgtattccg ctcgattcga tcacggattg gcggaatacc taactgtgat tgattcaggt
      241 accattttgc agggatccgg tgcgttactg tctgacacaa caaacgcagc gggtcgctct
      301 gctccgccga ttcctgataa tccctcccca gtaacctact gcaacagtac aaatacactg
      361 acgcctgaaa cggtaaccag aatcagcacc agcagtaact cagtaaacag agcagacgat
      421 cggtcttccg gtaataaccg gaaggaacga cagcgccaca atacaggcaa cacactgtat
      481 tatgagtaat cgaattaccg caatccccgc atttcttttt gcgtatcacg atagctccct
      541 cgccacgcct tatccgtaac cggtttttta cattaaaaaa aacaacgccg ggaattcaat
      601 cgtcagtccg gagacgaccg ttcgggttat cacagagagc ctgagcaggg gggctgcgca
      661 ttttttacga tgtggctatt ccgtatgaac catacggaga tataaccatg acctacaaat
      721 acaacccctt ctggcagcaa cgtattcgtg agacggtgcg gcacgcactg aatgttcatc
      781 cccgcctgac ggcattgcgg gttgacctgc gtttcccgga tgtaccggca gcaacggacg
      841 cagctgtgat atcccgcttc atcaatgccc tgaaagcccg aatcgacgct taccagaaac
      901 gtaagcatcg ggaaggtaaa cgcgtgcatc ccac
//
LOCUS       DAZKHF020000606          931 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2201S31241
            SAMN43267800-rid25106413.denovo.610, whole genome shotgun sequence.
ACCESSION   DAZKHF020000606 DAZKHF020000000
VERSION     DAZKHF020000606.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267800
            Sequence Read Archive: SRR30307263
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 931)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 931)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 931)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350581.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350581.2
            Genome Coverage       :: 151x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:21:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,652
            CDSs (total)                      :: 5,557
            Genes (coding)                    :: 5,320
            CDSs (with protein)               :: 5,320
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 237
            CDSs (without protein)            :: 237
            Pseudo Genes (ambiguous residues) :: 0 of 237
            Pseudo Genes (frameshifted)       :: 102 of 237
            Pseudo Genes (incomplete)         :: 130 of 237
            Pseudo Genes (internal stop)      :: 52 of 237
            Pseudo Genes (multiple problems)  :: 41 of 237
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..931
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267800-rid25106413.denovo.610"
                     /serotype="O 26:H11"
                     /isolate="2201S31241"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-01"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            <1..117
                     /locus_tag="ACHOSC_RS26205"
                     /old_locus_tag="pgaptmp_005241"
     CDS             <1..117
                     /locus_tag="ACHOSC_RS26205"
                     /old_locus_tag="pgaptmp_005241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gpW family protein"
                     /protein_id="HGU4550966.1"
                     /translation="RRIEYTAASLDDLNRAINDAESVLGTTSRRRRPLGVRL"
     gene            114..>931
                     /locus_tag="ACHOSC_RS26210"
                     /old_locus_tag="pgaptmp_005242"
     CDS             114..>931
                     /locus_tag="ACHOSC_RS26210"
                     /old_locus_tag="pgaptmp_005242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HGU4550967.1"
                     /translation="MKRTPVLIDVNGVPLRESLSYNGGGAGFGGQMAEWLPPAQSADA
                     ALLPALRLGNARADDLVRNNGIAANAVALHKDHIVGHMFLISYRPNWRWLGMRETAAK
                     SFVDEVEAAWSEYAEGMSGEIDVEGKRTFTEFIREGVGVHAFNGEIFVQPVWDTESTQ
                     LFRTRFKAVSPKRVDTPGHGMGNRFLRAGVEVDRYGRAVAYHICEDDFPFSGSGRWER
                     IPRELPTGRPAMLHIFEPVEDGQTRGANQFYSVMERLKMLDSLQATQLQSAIVKA"
BASE COUNT          191 a          216 c          322 g          202 t
ORIGIN      
        1 cgaagaattg aatatacggc ggcctctctg gatgatctta accgggcgat caatgatgcg
       61 gagtcagtgc tggggacaac aagccgccgc cgtcgtccgc tgggagtgag gttatgaaac
      121 gaacgcctgt cctgattgat gtgaacggtg ttccgcttcg ggagagcctc agctacaacg
      181 ggggcggtgc aggatttggc gggcaaatgg cggagtggtt gccaccggcg cagagtgccg
      241 atgcggccct gttgcccgcg ttgcgtctgg gcaatgcccg tgcagatgat ctggtgcgca
      301 ataacgggat agcggccaat gcggtggcac tgcataagga tcatattgtc gggcatatgt
      361 ttctgatcag ctaccgtccg aactggcgct ggctggggat gcgggagact gcagcaaaaa
      421 gttttgtcga tgaggtggag gcggcctggt cggaatacgc cgaagggatg tctggcgaga
      481 tcgacgtgga agggaaacgc acgtttacgg aatttatccg tgaaggtgtg ggcgttcatg
      541 cgtttaacgg cgaaatcttt gtgcagccgg tctgggatac ggagagcacg caactgtttc
      601 gtacgcgttt taaagccgtg agtccgaaac gggtggacac gccaggacac ggtatgggga
      661 accgttttct gcgggccggg gtggaggtcg atcgatatgg ccgtgccgtt gcgtaccata
      721 tctgtgagga tgattttccg ttctctggta gtggacgatg ggaacggatc ccgcgtgaac
      781 tacccaccgg gcgtccggcc atgctgcata ttttcgagcc ggtggaggac gggcagaccc
      841 gtggggctaa tcagttttac agcgtcatgg aacggctgaa gatgctggat tccctgcagg
      901 caacacagct tcagtcggcc attgtgaaag c
//
LOCUS       DAZKHF020000607          931 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2201S31241
            SAMN43267800-rid25106413.denovo.611, whole genome shotgun sequence.
ACCESSION   DAZKHF020000607 DAZKHF020000000
VERSION     DAZKHF020000607.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267800
            Sequence Read Archive: SRR30307263
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 931)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 931)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 931)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350581.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350581.2
            Genome Coverage       :: 151x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:21:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,652
            CDSs (total)                      :: 5,557
            Genes (coding)                    :: 5,320
            CDSs (with protein)               :: 5,320
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 237
            CDSs (without protein)            :: 237
            Pseudo Genes (ambiguous residues) :: 0 of 237
            Pseudo Genes (frameshifted)       :: 102 of 237
            Pseudo Genes (incomplete)         :: 130 of 237
            Pseudo Genes (internal stop)      :: 52 of 237
            Pseudo Genes (multiple problems)  :: 41 of 237
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..931
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267800-rid25106413.denovo.611"
                     /serotype="O 26:H11"
                     /isolate="2201S31241"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-01"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            <1..117
                     /gene="gpW"
                     /locus_tag="ACHOSC_RS26215"
                     /old_locus_tag="pgaptmp_005243"
     CDS             <1..117
                     /gene="gpW"
                     /locus_tag="ACHOSC_RS26215"
                     /old_locus_tag="pgaptmp_005243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0019058 - viral life cycle [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gpW family head-tail joining protein"
                     /protein_id="HGU4550968.1"
                     /translation="RRIEYTAASLDELNRAINDAESVLGTTRRRRRPLGVRL"
     gene            114..>931
                     /locus_tag="ACHOSC_RS26220"
                     /old_locus_tag="pgaptmp_005244"
     CDS             114..>931
                     /locus_tag="ACHOSC_RS26220"
                     /old_locus_tag="pgaptmp_005244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HGU4550969.1"
                     /translation="MKRTPVLIDVNGVPLRESLSYNGGGAGFGGQMAEWLPPAQSADA
                     ALLPALRLGNARADDLVRNNGIAANAVALHKDHIVGHMFLISYRPNWRWLGMRETAAK
                     SFVDEVEAAWSEYAEGMSGEIDVEGKRTFTEFIREGVGVHAFNGEIFVQPVWDTETTQ
                     LFRTRFKAVSPKRVDTPGHGMGNRFLRAGVEVDRYGRAVAYHICEDDFPFSGSGRWER
                     IPRELPTGRPAMLHIFEPVEDGQTRGANQFYSVMERLKMLDSLQATQLQSAIVKA"
BASE COUNT          189 a          224 c          321 g          197 t
ORIGIN      
        1 cgcagaattg aatatacggc ggcttctctg gatgagctta accgggcgat caatgatgcg
       61 gagtcggtac tggggacaac ccggcgtcgc cgtcgtccgc tgggagtgag gttatgaaac
      121 gaacgcctgt cctgattgat gtgaacggcg ttccgcttcg tgagagtctc agctacaacg
      181 ggggcggtgc aggatttggc gggcaaatgg ctgagtggtt gccaccggcg cagagtgccg
      241 atgcggccct gctgcccgcg ttgcgtctgg ggaatgcccg ggcagatgat ctggtgcgca
      301 ataacggaat agcggctaat gcggtggctc tgcataagga tcacattgtc gggcatatgt
      361 ttctgatcag ctaccgtccg aactggcgct ggctggggat gcgggagacc gcagcaaaaa
      421 gctttgtcga tgaggtggag gcggcctggt cggaatacgc cgaagggatg tctggcgaga
      481 tcgacgtgga aggaaaacgc acgttcacgg aatttatccg tgaaggtgtg ggcgttcatg
      541 cgtttaacgg cgaaatcttt gtgcagccgg tctgggatac ggaaaccacg cagttattcc
      601 gtacgcgttt taaagccgtg agtccgaaac gggtggacac gccaggacac ggtatgggga
      661 accgttttct gcgggccggg gtggaggtcg atcgatatgg ccgtgccgtt gcgtaccata
      721 tctgtgagga tgattttccg ttctccggga gtggacgatg ggaacggatc ccgcgtgaac
      781 ttcccaccgg gcgtccggcc atgctgcata ttttcgagcc ggtggaggac gggcagaccc
      841 gtggggccaa ccagttttac agcgtcatgg aacggctgaa gatgctcgat tccctgcagg
      901 caacacagct tcagtcggcc atagtgaagg c
//
LOCUS       DAZKHF020000608          931 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2201S31241
            SAMN43267800-rid25106413.denovo.612, whole genome shotgun sequence.
ACCESSION   DAZKHF020000608 DAZKHF020000000
VERSION     DAZKHF020000608.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267800
            Sequence Read Archive: SRR30307263
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 931)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 931)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 931)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350581.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350581.2
            Genome Coverage       :: 151x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:21:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,652
            CDSs (total)                      :: 5,557
            Genes (coding)                    :: 5,320
            CDSs (with protein)               :: 5,320
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 237
            CDSs (without protein)            :: 237
            Pseudo Genes (ambiguous residues) :: 0 of 237
            Pseudo Genes (frameshifted)       :: 102 of 237
            Pseudo Genes (incomplete)         :: 130 of 237
            Pseudo Genes (internal stop)      :: 52 of 237
            Pseudo Genes (multiple problems)  :: 41 of 237
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..931
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267800-rid25106413.denovo.612"
                     /serotype="O 26:H11"
                     /isolate="2201S31241"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-01"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            143..376
                     /locus_tag="ACHOSC_RS26225"
                     /old_locus_tag="pgaptmp_005245"
     CDS             143..376
                     /locus_tag="ACHOSC_RS26225"
                     /old_locus_tag="pgaptmp_005245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4550970.1"
                     /translation="MRDIYHETIDRAFSALAYAEGMYEILRIWLETLGDNERDKQKSR
                     IVTALITLLEPVINELQEIETLHDRYNEQHTGE"
     gene            382..681
                     /locus_tag="ACHOSC_RS26230"
                     /old_locus_tag="pgaptmp_005246"
     CDS             382..681
                     /locus_tag="ACHOSC_RS26230"
                     /old_locus_tag="pgaptmp_005246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4222 domain-containing protein"
                     /protein_id="HGU4550971.1"
                     /translation="MKLKYSGLTASGNTHPKFTRGDIYRDQYGGTVMIKGVAGRCVTY
                     RREGYEYDCVMPVYQFRRDFSLVQTAPHNVPTSNARARANIQKLKTMINGFRGKK"
     gene            678..>931
                     /locus_tag="ACHOSC_RS26235"
                     /old_locus_tag="pgaptmp_005247"
     CDS             678..>931
                     /locus_tag="ACHOSC_RS26235"
                     /old_locus_tag="pgaptmp_005247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000833619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA primase"
                     /protein_id="HGU4550972.1"
                     /translation="MKSAPNLKKQPYDKMTEVIIFAGSDAWAHAKQWQEQDGRLAGDN
                     VPPVVLADDQLDELADLRIIDEGRYCVRLYKAGHIRPSNI"
BASE COUNT          281 a          200 c          235 g          215 t
ORIGIN      
        1 tctgaaatta tgcgggtggc ttttttcttt ctttgcactg atgagcacag agatacaggg
       61 ctaaatattc ttgatattgc cgctaactat gctgatttcg tgacagaagc tgttttaaga
      121 aaaacaacgg acggggatta atatgcgtga tatttaccac gaaacaatag accgcgcatt
      181 tagtgccctt gcttacgctg aaggtatgta cgaaatattg cgcatatggc ttgaaacact
      241 tggcgacaat gaacgcgaca aacaaaaatc aagaattgtc acggcattaa taacgcttct
      301 tgagcctgta ataaatgagt tgcaggaaat agagacattg cacgacagat ataacgaaca
      361 gcacaccgga gaataaaaat aatgaaactt aaatattctg gcttaactgc cagtggcaac
      421 actcacccta aatttacgcg cggtgatatt taccgcgacc agtacggcgg cacggtaatg
      481 attaagggcg tggcgggacg gtgcgtaact taccgccgtg aaggttacga atatgattgc
      541 gtgatgcctg tttatcagtt ccggcgtgat ttttctctgg tacagaccgc gccgcataac
      601 gtgcccacca gcaacgccag ggcacgggca aacatccaga agctgaaaac catgattaac
      661 ggattcaggg gcaagaaatg aaaagcgcac cgaacttaaa aaaacagcct tacgacaaga
      721 tgaccgaagt cattattttt gcgggtagtg atgcctgggc acatgcgaaa cagtggcagg
      781 aacaggacgg gcgactggct ggcgataatg tgcctcccgt tgtgctggct gatgatcaac
      841 tggatgaact ggcagacctg agaatcatcg acgaggggcg ctattgtgtc cggctgtaca
      901 aggcaggcca catcaggcca tcaaatatta a
//
LOCUS       DAZKHF020000609          929 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2201S31241
            SAMN43267800-rid25106413.denovo.613, whole genome shotgun sequence.
ACCESSION   DAZKHF020000609 DAZKHF020000000
VERSION     DAZKHF020000609.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267800
            Sequence Read Archive: SRR30307263
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 929)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 929)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 929)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350581.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350581.2
            Genome Coverage       :: 151x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:21:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,652
            CDSs (total)                      :: 5,557
            Genes (coding)                    :: 5,320
            CDSs (with protein)               :: 5,320
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 237
            CDSs (without protein)            :: 237
            Pseudo Genes (ambiguous residues) :: 0 of 237
            Pseudo Genes (frameshifted)       :: 102 of 237
            Pseudo Genes (incomplete)         :: 130 of 237
            Pseudo Genes (internal stop)      :: 52 of 237
            Pseudo Genes (multiple problems)  :: 41 of 237
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..929
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267800-rid25106413.denovo.613"
                     /serotype="O 26:H11"
                     /isolate="2201S31241"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-01"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            complement(<1..835)
                     /locus_tag="ACHOSC_RS26240"
                     /old_locus_tag="pgaptmp_005248"
     CDS             complement(<1..835)
                     /locus_tag="ACHOSC_RS26240"
                     /old_locus_tag="pgaptmp_005248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309150.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prophage tail fiber N-terminal domain-containing
                     protein"
                     /protein_id="HGU4550973.1"
                     /translation="MAVKISGVLKDGTGKPVENCTIQLKARRTSSTVVVNTVASENPD
                     EAGRYSMDVEYGQYSVILLVEGFPPSHAGTITVYEDSQPGTLNDFLGAMSEDDVRPEA
                     LRRFELMVEEVARHAEEAKKNAGEAETSARNAGISASQAEEGAANADTSAGEASESAR
                     QAAESAAAAKQSEEASSSSASAAAQKASESSQSAAEAELSRKTAESAAGNAARDATTA
                     TEKARESAESAQSAEQSRIAAEEAVNRIPTVVGPPGPKGEPGPAGPQGPKGDKGERGD
                     TG"
BASE COUNT          148 a          314 c          226 g          241 t
ORIGIN      
        1 ggccggtgtc gccacgctct cctttatctc ccttcggccc ctgaggaccc gcgggacccg
       61 gttccccctt tggcccggga ggccccacca cggtggggat tcggtttacg gcctcttccg
      121 ccgctatcct gctttgttcc gctgactgtg cgctttctgc tgactcccgg gctttttctg
      181 ttgcggtcgt tgcatccctg gctgcattac cggctgcact ttctgccgtc tttcttgaca
      241 attcagcttc tgctgcactt tgtgatgact cactggcttt ttgagcggcc gcagaggccg
      301 aggacgagga cgcttcctct gactgctttg cagcggctgc actttctgcc gcctgccggg
      361 ctgactccga tgcctcccct gctgaagtgt cagcatttgc agcgccctct tctgcctgac
      421 tggctgatat gccggcattc ctcgctgacg tctccgcctc tccggcattc ttcttcgcct
      481 cctctgcgtg acgcgccacc tcttccacca tcagttcaaa acggcgcagt gcctccggcc
      541 ggacgtcatc ctccgacatg gcaccgagaa aatcattcag cgtccccggt tgagaatctt
      601 catacacggt gatggtcccg gcatgtgacg gcgggaagcc ctccaccaac agaatgacgc
      661 tgtactgacc gtactcaacg tccatgctgt aacgaccggc ttcatccgga ttttcagagg
      721 ccaccgtatt caccaccacc gtgctgctgg tccgtctggc tttcagttga atggtgcagt
      781 tctctaccgg ttttcctgtg ccgtctttca gtacacctga aatctttact gccatattca
      841 ccccacaaaa aagcccgcct gaaccggcgg gctgtcataa cactgtgtta cctggctaat
      901 cagaatttat agccgacacc cacgatgaa
//
LOCUS       DAZKHF020000610          929 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2201S31241
            SAMN43267800-rid25106413.denovo.614, whole genome shotgun sequence.
ACCESSION   DAZKHF020000610 DAZKHF020000000
VERSION     DAZKHF020000610.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267800
            Sequence Read Archive: SRR30307263
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 929)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 929)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 929)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350581.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350581.2
            Genome Coverage       :: 151x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:21:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,652
            CDSs (total)                      :: 5,557
            Genes (coding)                    :: 5,320
            CDSs (with protein)               :: 5,320
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 237
            CDSs (without protein)            :: 237
            Pseudo Genes (ambiguous residues) :: 0 of 237
            Pseudo Genes (frameshifted)       :: 102 of 237
            Pseudo Genes (incomplete)         :: 130 of 237
            Pseudo Genes (internal stop)      :: 52 of 237
            Pseudo Genes (multiple problems)  :: 41 of 237
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..929
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267800-rid25106413.denovo.614"
                     /serotype="O 26:H11"
                     /isolate="2201S31241"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-01"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            complement(1..480)
                     /locus_tag="ACHOSC_RS26245"
                     /old_locus_tag="pgaptmp_005249"
                     /pseudo
     CDS             complement(1..480)
                     /locus_tag="ACHOSC_RS26245"
                     /old_locus_tag="pgaptmp_005249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311753.1"
                     /note="frameshifted; internal stop; incomplete; missing
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="YscQ/HrcQ family type III secretion apparatus
                     protein"
     gene            complement(440..559)
                     /locus_tag="ACHOSC_RS26250"
                     /old_locus_tag="pgaptmp_005250"
                     /pseudo
     CDS             complement(440..559)
                     /locus_tag="ACHOSC_RS26250"
                     /old_locus_tag="pgaptmp_005250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000344669.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion protein"
     gene            complement(572..>929)
                     /locus_tag="ACHOSC_RS26255"
                     /old_locus_tag="pgaptmp_005251"
     CDS             complement(572..>929)
                     /locus_tag="ACHOSC_RS26255"
                     /old_locus_tag="pgaptmp_005251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001443751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4550974.1"
                     /translation="HAKREKSFIPSTEAEWFFDSWYAKSAAIVYKESRKEEYLNDSVQ
                     FMNRSLAQITGENMIGANGRSVPEMALPESYNYIHKSGTLHEAPSPIIPLNWSNGSMT
                     LMLKEMSNLINDEGIK"
BASE COUNT          237 a          211 c          155 g          326 t
ORIGIN      
        1 tttttgtcta acactggagc gtttccgtat tggcagactg tgactttatc taaccaaaga
       61 ttcaattccc ctgttcatat tcttattagt ggttgagcag ggctcaatac tccatcaaat
      121 actttaccta cgtgcaaacg attatatttt agttcaggat gctttagatt taacggggaa
      181 ctggataaac tgagccattc ccctattaac ctttccgttt ctggcattaa agccatccca
      241 gcgagctcag atgaaattaa gggaaatatt tctccaagca atacccatcc agaccagctg
      301 ccactctctt ctgctatcgg taaccattta gcactaaatt cgggcattaa taatgcaaca
      361 tcttcgccat tttctttcca attttgaaaa gtggtttcaa acgtatgagt gtttcgattt
      421 actttcctca gaccgaacat taactttcat ccttctgatc gttttcgaat ttgctttcat
      481 tttcttgctc ctcataaggc tgatgtcgtt gtccttgtcg ttcatcttgc tcagtcaata
      541 cccaacgttg tgctgtaact cattatgaaa actatttaat tccctcatca ttgataaggt
      601 tggacatctc tttcaacatg agagtcattg aaccattcga ccaatttagc ggaatgatag
      661 ggcttggcgc ttcatgcaaa gttcccgact tgtggatata gttatagctt tcgggaagtg
      721 ccatttcagg tacactcctg ccatttgcac caatcatatt ctcgcctgtt atttgggcca
      781 atgatctatt cataaattgc actgagtcat tcaaatactc ttcctttctt gattctttat
      841 aaacaattgc tgctgatttt gcataccatg agtcaaaaaa ccattctgct tctgttgatg
      901 gaatgaagct cttttctctt ttcgcatgt
//
LOCUS       DAZKHF020000611          924 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2201S31241
            SAMN43267800-rid25106413.denovo.615, whole genome shotgun sequence.
ACCESSION   DAZKHF020000611 DAZKHF020000000
VERSION     DAZKHF020000611.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267800
            Sequence Read Archive: SRR30307263
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 924)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 924)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 924)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350581.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350581.2
            Genome Coverage       :: 151x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:21:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,652
            CDSs (total)                      :: 5,557
            Genes (coding)                    :: 5,320
            CDSs (with protein)               :: 5,320
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 237
            CDSs (without protein)            :: 237
            Pseudo Genes (ambiguous residues) :: 0 of 237
            Pseudo Genes (frameshifted)       :: 102 of 237
            Pseudo Genes (incomplete)         :: 130 of 237
            Pseudo Genes (internal stop)      :: 52 of 237
            Pseudo Genes (multiple problems)  :: 41 of 237
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..924
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267800-rid25106413.denovo.615"
                     /serotype="O 26:H11"
                     /isolate="2201S31241"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-01"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            <1..582
                     /locus_tag="ACHOSC_RS26260"
                     /old_locus_tag="pgaptmp_005252"
     CDS             <1..582
                     /locus_tag="ACHOSC_RS26260"
                     /old_locus_tag="pgaptmp_005252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030430 - host cell cytoplasm [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0046718 - symbiont entry into host cell
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage minor tail protein L"
                     /protein_id="HGU4550975.1"
                     /translation="NELNEKGEAVTWQGRQYQAYPIDGSGFEMNGKGSSARPSLTVSN
                     LFGLVTGMAEDLQSLVGATVVRRRVYARFLDAVNFVAGNPEADPEQELSDRWVVEQMS
                     ELTAMTASFVLATPTETDGALFPGRIMLANTCMWDYRGDECGYNGPAVADEFDKPTTD
                     IRKDRCSKCMRGCEMRGMVANFGGFLSINKLSQ"
     gene            588..>924
                     /locus_tag="ACHOSC_RS26265"
                     /old_locus_tag="pgaptmp_005253"
     CDS             588..>924
                     /locus_tag="ACHOSC_RS26265"
                     /old_locus_tag="pgaptmp_005253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C40 family peptidase"
                     /protein_id="HGU4550976.1"
                     /translation="MTQTESAILAHARRCVPAESCGFVVRTPEGERYIPCVNISAEPE
                     AYFRIAPEDWLRAEMQGEIVALVHSHPGGLPWLSEADRRLQIKSALPWWLVCRGDIHK
                     FRCVPHLTGR"
BASE COUNT          186 a          221 c          330 g          187 t
ORIGIN      
        1 aatgagctga atgaaaaagg ggaggcggtt acctggcagg ggcggcaata tcaggcatac
       61 ccgattgacg gcagtggctt tgagatgaac gggaagggca gcagtgccag accgtcgctg
      121 acggtgtcga atctgttcgg tctggtcacc ggaatggcgg aggacctgca gagcctggtg
      181 ggggccacgg tggtccgccg ccgggtgtat gcccgttttc tggatgcggt gaattttgtg
      241 gcgggcaatc cggaagcgga cccggagcag gagctgagcg accgctgggt ggtggagcag
      301 atgtcagagc tgacggccat gacagcctcg tttgtgctgg caacaccgac cgagacggat
      361 ggggcgctgt ttcccggtcg catcatgctg gcgaacacct gtatgtggga ttaccgggga
      421 gatgaatgcg ggtataacgg tcctgcggtg gcggatgagt tcgataaacc caccaccgat
      481 atccgtaagg acagatgcag caagtgcatg cgcgggtgtg agatgcgcgg catggtggct
      541 aattttggcg gtttcctttc cattaacaaa ctttcgcagt aaatccaatg acacagacag
      601 aatcagcgat tctggcgcat gcccggcggt gtgtgcctgc ggagtcgtgc ggcttcgtgg
      661 tgagaacgcc ggagggggag cggtatatcc cttgtgtgaa tatctctgca gagccggagg
      721 cgtattttcg tattgcaccg gaagactggc tgcgggcaga gatgcagggg gagattgtgg
      781 cactggtcca cagtcatccc ggtgggctgc cctggctgag cgaggctgac cggcggctgc
      841 agataaaaag cgcactgccc tggtggctgg tctgccgggg tgacattcac aaattccgct
      901 gtgtgccaca tctgacagga cggc
//
LOCUS       DAZKHF020000612          924 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2201S31241
            SAMN43267800-rid25106413.denovo.616, whole genome shotgun sequence.
ACCESSION   DAZKHF020000612 DAZKHF020000000
VERSION     DAZKHF020000612.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267800
            Sequence Read Archive: SRR30307263
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 924)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 924)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 924)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350581.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350581.2
            Genome Coverage       :: 151x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:21:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,652
            CDSs (total)                      :: 5,557
            Genes (coding)                    :: 5,320
            CDSs (with protein)               :: 5,320
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 237
            CDSs (without protein)            :: 237
            Pseudo Genes (ambiguous residues) :: 0 of 237
            Pseudo Genes (frameshifted)       :: 102 of 237
            Pseudo Genes (incomplete)         :: 130 of 237
            Pseudo Genes (internal stop)      :: 52 of 237
            Pseudo Genes (multiple problems)  :: 41 of 237
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..924
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267800-rid25106413.denovo.616"
                     /serotype="O 26:H11"
                     /isolate="2201S31241"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-01"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            complement(<1..449)
                     /locus_tag="ACHOSC_RS26270"
                     /old_locus_tag="pgaptmp_005254"
     CDS             complement(<1..449)
                     /locus_tag="ACHOSC_RS26270"
                     /old_locus_tag="pgaptmp_005254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001704620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4550977.1"
                     /translation="MIFRDSLRVLPYGRVDNDFFQIEERRSWNAGRYYWSNRRIFGYI
                     GITQSSNKELKDKSGREGFIRNQAARELKTIISNLLTELADRFFGSRSDDRKELLEQV
                     KREKELRKSAQQQARKSTQKSFSEALKNQTPVLDASLEAVKRLKTKL"
     gene            complement(601..>924)
                     /locus_tag="ACHOSC_RS26275"
                     /old_locus_tag="pgaptmp_005255"
     CDS             complement(601..>924)
                     /locus_tag="ACHOSC_RS26275"
                     /old_locus_tag="pgaptmp_005255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HGU4550978.1"
                     /translation="WRRKRPRNVIVHTDRGGQYCSADYQAQLKRHNLRGSMSAKGCCY
                     DNACVESFFHSLKVECIHGEHFISREIMRATVFNYIECDYNRWRRHSWCGGLSPEQFE
                     NKNLA"
BASE COUNT          243 a          229 c          163 g          289 t
ORIGIN      
        1 tcaagcttag ttttcagcct tttaacagcc tccaaggaag catcaagaac tggtgtctga
       61 ttcttcaaag cttctgaaaa acttttttgt gttgattttc gagcttgttg ttgagcagat
      121 tttcttaact ctttttcacg cttaacttgt tctaaaagct ctttacggtc atcagaacgt
      181 gaaccaaaaa atctatcagc aagttcagtt aacaaattag atataatagt tttcagttct
      241 cttgcagcct ggtttcttat gaatccctct ctccccgact tatctttcag ctctttatta
      301 ctggattgag taattccaat ataaccaaaa atccttctat tagaccaata atatcgccct
      361 gcattccatg aacgtctttc ttctatctgg aagaaatcat tatctactcg accataaggt
      421 aatactctca agctatccct aaaaatcatt agtcctgcat actttttggc cttaagatca
      481 aagtgggaat gttcacgttc agtatgtgat gtattttgtg aaaggaattc aaacgttcct
      541 atctgaagct caaatgggcc gccccctgcg tgatcctacc cacgtaatat ggacacaggc
      601 ctaagcgagg ttcttgtttt caaattgttc cggactgagg ccgccacacc aactgtgccg
      661 ccgccaccga ttgtaatcac attcgatata attaaacacc gttgcccgca ttatttcccg
      721 gctgataaag tgttctccat ggatacattc cactttcagc gaatgaaaga agctttccac
      781 gcaggcatta tcgtagcagc aaccttttgc gctcatactt ccacgcagat tatgccgctt
      841 cagttgcgcc tgataatctg ctgaacagta ctggcctcca cggtccgtgt gaacgataac
      901 gttccggggc ctcttacgcc gcca
//
LOCUS       DAZKHF020000613          924 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2201S31241
            SAMN43267800-rid25106413.denovo.617, whole genome shotgun sequence.
ACCESSION   DAZKHF020000613 DAZKHF020000000
VERSION     DAZKHF020000613.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267800
            Sequence Read Archive: SRR30307263
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 924)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 924)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 924)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350581.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350581.2
            Genome Coverage       :: 151x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:21:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,652
            CDSs (total)                      :: 5,557
            Genes (coding)                    :: 5,320
            CDSs (with protein)               :: 5,320
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 237
            CDSs (without protein)            :: 237
            Pseudo Genes (ambiguous residues) :: 0 of 237
            Pseudo Genes (frameshifted)       :: 102 of 237
            Pseudo Genes (incomplete)         :: 130 of 237
            Pseudo Genes (internal stop)      :: 52 of 237
            Pseudo Genes (multiple problems)  :: 41 of 237
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..924
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267800-rid25106413.denovo.617"
                     /serotype="O 26:H11"
                     /isolate="2201S31241"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-01"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            <1..659
                     /locus_tag="ACHOSC_RS26280"
                     /old_locus_tag="pgaptmp_005256"
     CDS             <1..659
                     /locus_tag="ACHOSC_RS26280"
                     /old_locus_tag="pgaptmp_005256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000788752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HGU4550979.1"
                     /translation="EEWREWQLAEGRKRSEEINRLNHQVRVEKILNRAGIQPLHRKCS
                     FGNYRVQNDGQRHALSQAKSIADELMTGCTNFVFSGKPGTGKNHLAAAIGNRLMAKGR
                     SVIIVTVSDVMSVLHDGYDNGQSGEKFLQELCGVDLLVLDEIGMQRDTRNEQVTLNQI
                     VDRRTASMRSVGMLTNLNHAAMSTLLGDRVMDRMTMNGGRWVNFNWESWRSNVGRQGM
                     "
     gene            685..>924
                     /locus_tag="ACHOSC_RS26285"
                     /old_locus_tag="pgaptmp_005257"
     CDS             685..>924
                     /locus_tag="ACHOSC_RS26285"
                     /old_locus_tag="pgaptmp_005257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000450870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4550980.1"
                     /translation="METVLHALKAMGKANSVELAARLDISREEVLNELWELKKNGVVD
                     KTGHTWFLAVEGEAGVTEGQALQPEAPDVVTEEVAP"
BASE COUNT          243 a          197 c          290 g          194 t
ORIGIN      
        1 tggaggagtg gcgggaatgg caacttgctg aaggacgaaa acgcagcgag gagattaacc
       61 gcctgaatca tcaggtgcgg gttgaaaaaa tcctgaaccg tgcgggcatc cagccgcttc
      121 acaggaagtg ctcattcggg aactaccggg tgcagaacga cggtcagcgc catgctctga
      181 gccaggcgaa atccattgcc gatgaattga tgaccggatg tacaaacttc gtgttcagcg
      241 gtaaacctgg taccggtaaa aatcacctgg cagcagcgat tggcaatcgg ctgatggcga
      301 aggggagaag cgtgattatc gtcaccgtgt ccgatgtcat gagcgtgttg catgacggct
      361 acgacaacgg ccagtccggg gaaaaatttt tacaggagct ttgtggagtt gaccttctgg
      421 tccttgatga aattggcatg cagcgggata cgcgcaacga gcaggtcacg ctgaaccaga
      481 tagtcgaccg cagaacggct tcgatgcgta gtgtcggaat gctgacgaac ctgaatcacg
      541 cagcgatgag cacactcctc ggagatcggg tgatggaccg tatgaccatg aatggtggtc
      601 gttgggtgaa ttttaactgg gagagctggc ggtcaaacgt tggacgtcag ggtatgtgag
      661 aatttttgac gaggtaaatt ttcgatggaa actgtattgc atgcactgaa agcgatggga
      721 aaagccaatt ctgttgaact ggcggcgcgg cttgatatca gccgtgaaga agttcttaac
      781 gaactgtggg aactcaaaaa aaatggcgtt gttgataaaa cgggtcacac ctggtttctg
      841 gctgtcgaag gtgaagccgg ggtaaccgaa gggcaggcac tacaacctga agcgccggat
      901 gtggtaaccg aagaggtcgc tcca
//
LOCUS       DAZKHF020000614          916 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2201S31241
            SAMN43267800-rid25106413.denovo.618, whole genome shotgun sequence.
ACCESSION   DAZKHF020000614 DAZKHF020000000
VERSION     DAZKHF020000614.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267800
            Sequence Read Archive: SRR30307263
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 916)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 916)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 916)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350581.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350581.2
            Genome Coverage       :: 151x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:21:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,652
            CDSs (total)                      :: 5,557
            Genes (coding)                    :: 5,320
            CDSs (with protein)               :: 5,320
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 237
            CDSs (without protein)            :: 237
            Pseudo Genes (ambiguous residues) :: 0 of 237
            Pseudo Genes (frameshifted)       :: 102 of 237
            Pseudo Genes (incomplete)         :: 130 of 237
            Pseudo Genes (internal stop)      :: 52 of 237
            Pseudo Genes (multiple problems)  :: 41 of 237
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..916
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267800-rid25106413.denovo.618"
                     /serotype="O 26:H11"
                     /isolate="2201S31241"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-01"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            61..495
                     /locus_tag="ACHOSC_RS26290"
                     /old_locus_tag="pgaptmp_005258"
     CDS             61..495
                     /locus_tag="ACHOSC_RS26290"
                     /old_locus_tag="pgaptmp_005258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001556209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-ribbon domain-containing protein"
                     /protein_id="HGU4550981.1"
                     /translation="MRILNCYMANDSKGHFVTAKEAAKHNRQDVLCCVSCGCPLTLQR
                     GNDGQPPWFEHDQMTVAEKILLRCTWLDPAEKEARRLHLQGMTVPDYTVKVRKWFCVM
                     CDEDYEGEKCCPRCGTGVYSRAWGRQEVPSEDARADNPLQRL"
BASE COUNT          221 a          204 c          275 g          216 t
ORIGIN      
        1 gtactgcaca cactgccaga ccggaggatt cagtattccc cggacagctc aggaggaaat
       61 atgcgaattc tgaactgtta tatggcgaat gacagcaaag gccattttgt tacggcgaaa
      121 gaagctgcga agcacaaccg acaggacgtt ttgtgctgtg tgtcctgtgg atgcccgtta
      181 acacttcagc ggggcaatga cggacaacca ccgtggtttg aacatgacca gatgactgtc
      241 gctgaaaaaa tcctgctgcg atgtacctgg cttgacccgg cggagaaaga ggcccgtcgt
      301 ttgcatctgc agggcatgac ggttccggat tatacggtga aggtgagaaa gtggttttgt
      361 gtgatgtgtg acgaagatta tgagggggaa aagtgctgcc cacgctgcgg taccggggta
      421 tacagcaggg cgtgggggcg gcaggaggtg ccgtcggaag atgccagggc tgataatccg
      481 ttacagaggc tgtaatggtt gcctccggag cagtttgcgg tgatgccttt cccgggaatg
      541 gattgccagt gcggggactg tggagcttaa ttccggtgtt ggtggccttt ccgttgattt
      601 tatgccaaca gccccctgtg actgacaggc tgcctcgtca ttccattcgt atctccagta
      661 acagggggtt gtattgtatt ttattgtgcc ggttcaggtg tgcgtttccc ggcgcgtctg
      721 caccggctta accaaattca acagggataa aatagtggta agcgtacagc ctgaaccgtc
      781 tggtcagaat ctgacgaatt agacaaagtg gtgtccacca aataagtagt gggaaccaaa
      841 gtgtcagata tgcagaaaaa tgtgactccc ggcaggcgaa aaggctgccc taattatcct
      901 cccgaattta aacagc
//
LOCUS       DAZKHF020000615          913 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2201S31241
            SAMN43267800-rid25106413.denovo.619, whole genome shotgun sequence.
ACCESSION   DAZKHF020000615 DAZKHF020000000
VERSION     DAZKHF020000615.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267800
            Sequence Read Archive: SRR30307263
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 913)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 913)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 913)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350581.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350581.2
            Genome Coverage       :: 151x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:21:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,652
            CDSs (total)                      :: 5,557
            Genes (coding)                    :: 5,320
            CDSs (with protein)               :: 5,320
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 237
            CDSs (without protein)            :: 237
            Pseudo Genes (ambiguous residues) :: 0 of 237
            Pseudo Genes (frameshifted)       :: 102 of 237
            Pseudo Genes (incomplete)         :: 130 of 237
            Pseudo Genes (internal stop)      :: 52 of 237
            Pseudo Genes (multiple problems)  :: 41 of 237
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..913
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267800-rid25106413.denovo.619"
                     /serotype="O 26:H11"
                     /isolate="2201S31241"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-01"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            complement(<1..297)
                     /locus_tag="ACHOSC_RS26295"
                     /old_locus_tag="pgaptmp_005259"
     CDS             complement(<1..297)
                     /locus_tag="ACHOSC_RS26295"
                     /old_locus_tag="pgaptmp_005259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tail fiber assembly protein"
                     /protein_id="HGU4550982.1"
                     /translation="MAFRMSEQPRTIKIYNLLAGTNEFIGEGDAYIPPHTGLPANSTD
                     IAPPDIPAGFVAVFNSDKASWHLVEDHRGKTVYDVASGDALFISELGPLPENVTW"
     gene            complement(297..>913)
                     /locus_tag="ACHOSC_RS26300"
                     /old_locus_tag="pgaptmp_005260"
     CDS             complement(297..>913)
                     /locus_tag="ACHOSC_RS26300"
                     /old_locus_tag="pgaptmp_005260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4550983.1"
                     /translation="FDKSAYPKLAAAYPSGVIPDMRGWTIKGKPASGRAVLSQEQDGI
                     KSHTHSASASSTDLGTKTTSSFDYGTKSTNNTGAHTHSLSGSTNAAGNHSHRDGRRFN
                     PSVFKDTYQYGYTSSGQNTWGVQGSVGMSTGWLANTSTDGNHSHSLSGTAASAGAHAH
                     TVGIGAHTHSVAIGSHGHTITVNAAGNAENTVKNIAFNYIVRLA"
BASE COUNT          227 a          222 c          215 g          249 t
ORIGIN      
        1 ccaggtaaca ttttccggta acggaccgag ttcagaaata aataacgcgt cccctgacgc
       61 tacgtcataa accgttttac cccgatggtc ttcaacgaga tgccacgatg ccttatcact
      121 gttgaaaaca gccacaaagc cagcaggaat atctggtggt gcaatatcgg tactgtttgc
      181 tggcagacct gtatgaggcg gaatatatgc gtcaccttca ccaataaatt cattagttcc
      241 ggccagcaga ttataaattt ttatggtccg tggttgttca ctcattctga atgccattat
      301 gcaagcctca caatatagtt aaatgcgatg tttttgacgg tgttttccgc gttaccagca
      361 gcgttaacgg tgatggtgtg tccatgtgaa ccaatcgcaa cggagtgcgt atgagcacca
      421 ataccgacag tatgcgcgtg tgcacctgca gatgctgctg tgccggacag tgaatggcta
      481 tgattaccat ctgtactggt attcgctaac caccccgtag acatacctac tgagccttgt
      541 acaccccagg tattttgacc tgagcttgta taaccatatt gataagtatc tttaaaaaca
      601 ctggggttaa atcgacggcc atctctatgg ctgtgattac cagctgcatt cgtgctgcca
      661 cttaaactat gggtatgcgc accagtgtta ttcgtggatt tagtgccgta atcaaacgac
      721 gatgtggttt tcgtccccaa atccgtactg gatgcgctgg cgctgtgggt atgcgattta
      781 atgccgtcct gttcctgaga caatacggcc cgaccactgg caggtttgcc cttaatcgtc
      841 cagccacgca tatcagggat cacgcctgac ggataagccg ctgcaagttt cgggtatgca
      901 gatttgtcaa aag
//
LOCUS       DAZKHF020000616          897 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2201S31241
            SAMN43267800-rid25106413.denovo.620, whole genome shotgun sequence.
ACCESSION   DAZKHF020000616 DAZKHF020000000
VERSION     DAZKHF020000616.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267800
            Sequence Read Archive: SRR30307263
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 897)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 897)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 897)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350581.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350581.2
            Genome Coverage       :: 151x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:21:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,652
            CDSs (total)                      :: 5,557
            Genes (coding)                    :: 5,320
            CDSs (with protein)               :: 5,320
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 237
            CDSs (without protein)            :: 237
            Pseudo Genes (ambiguous residues) :: 0 of 237
            Pseudo Genes (frameshifted)       :: 102 of 237
            Pseudo Genes (incomplete)         :: 130 of 237
            Pseudo Genes (internal stop)      :: 52 of 237
            Pseudo Genes (multiple problems)  :: 41 of 237
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..897
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267800-rid25106413.denovo.620"
                     /serotype="O 26:H11"
                     /isolate="2201S31241"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-01"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            133..321
                     /gene="dicB"
                     /locus_tag="ACHOSC_RS26305"
                     /old_locus_tag="pgaptmp_005261"
     CDS             133..321
                     /gene="dicB"
                     /locus_tag="ACHOSC_RS26305"
                     /old_locus_tag="pgaptmp_005261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division inhibition protein DicB"
                     /protein_id="HGU4550984.1"
                     /translation="METLLPNVNTSEGCFDIGVLLSNREFTEDAINMRKYEPYLLNDN
                     SILSRIALLELGIFGERQ"
     gene            318..506
                     /locus_tag="ACHOSC_RS26310"
                     /old_locus_tag="pgaptmp_005262"
     CDS             318..506
                     /locus_tag="ACHOSC_RS26310"
                     /old_locus_tag="pgaptmp_005262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1482 family protein"
                     /protein_id="HGU4550985.1"
                     /translation="MTSAFALMMTVFLITGESQNVITGIYASKESCLQARDEQKISGE
                     CLPLKKVSLYLNNETPAG"
     gene            602..>897
                     /locus_tag="ACHOSC_RS26315"
                     /old_locus_tag="pgaptmp_005263"
     CDS             602..>897
                     /locus_tag="ACHOSC_RS26315"
                     /old_locus_tag="pgaptmp_005263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease"
                     /protein_id="HGU4550986.1"
                     /translation="MSKVFICAAIPDEQAIKEDSAVAVATAIEAGDERRARAKFHWQF
                     LEQFPAAQDCAYKFIVCEDKPGIPRPALDSWDAEYMQENRWDEASASFVPVETES"
BASE COUNT          256 a          193 c          204 g          244 t
ORIGIN      
        1 cacgcggaac agttaaaacc aaaaacagtg ttatgggtgg attctcttta tccggcgtta
       61 attgttaact ggttaacgtc acctggaggc accaggcact gcatcacaaa attcattgtt
      121 gaggacgcga taatggaaac gttattacca aacgttaata cgtctgaagg ttgttttgat
      181 attggtgttc tgctcagtaa ccgggagttt actgaagatg ccattaatat gaggaaatat
      241 gagccttatc tgctcaatga taattccata ctttcccgaa ttgctcttct tgaacttggt
      301 attttcggag aacgtcaatg acttcagcat ttgcactgat gatgacggtt tttcttataa
      361 cgggtgaatc acagaatgtg attaccggaa tttatgcaag taaagaatcc tgcctccagg
      421 caagagacga gcaaaaaatt tctggtgaat gcctcccgct aaaaaaagta tcgctgtacc
      481 tgaataacga aacaccggct ggataatcct ccagccatat taacaccata ccaacggatt
      541 aaaaatgcca gcaatggcag ggattcgttc accctgaaat ctgtaatgag gttaaaacaa
      601 aatgagtaag gtctttattt gcgctgctat tcctgatgaa caggccataa aagaagatag
      661 cgctgttgcg gtggccactg ccattgaagc tggtgatgag cgtcgcgcac gcgcaaaatt
      721 tcactggcaa tttctggagc aattccctgc agctcaggac tgcgcttata aatttattgt
      781 ctgtgaggat aaacccggca taccccgccc tgccctcgat tcctgggatg ctgaatatat
      841 gcaggaaaac cgctgggatg aggcgtctgc ttcctttgtc ccggttgaga ctgaatc
//
LOCUS       DAZKHF020000617          896 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2201S31241
            SAMN43267800-rid25106413.denovo.621, whole genome shotgun sequence.
ACCESSION   DAZKHF020000617 DAZKHF020000000
VERSION     DAZKHF020000617.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267800
            Sequence Read Archive: SRR30307263
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 896)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 896)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 896)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350581.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350581.2
            Genome Coverage       :: 151x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:21:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,652
            CDSs (total)                      :: 5,557
            Genes (coding)                    :: 5,320
            CDSs (with protein)               :: 5,320
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 237
            CDSs (without protein)            :: 237
            Pseudo Genes (ambiguous residues) :: 0 of 237
            Pseudo Genes (frameshifted)       :: 102 of 237
            Pseudo Genes (incomplete)         :: 130 of 237
            Pseudo Genes (internal stop)      :: 52 of 237
            Pseudo Genes (multiple problems)  :: 41 of 237
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..896
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267800-rid25106413.denovo.621"
                     /serotype="O 26:H11"
                     /isolate="2201S31241"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-01"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            <1..642
                     /locus_tag="ACHOSC_RS26320"
                     /old_locus_tag="pgaptmp_005264"
     CDS             <1..642
                     /locus_tag="ACHOSC_RS26320"
                     /old_locus_tag="pgaptmp_005264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015473 - fimbrial usher porin activity
                     [Evidence IEA];
                     GO_process: GO:0009297 - pilus assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbria/pilus outer membrane usher protein"
                     /protein_id="HGU4550987.1"
                     /translation="QNNDTSLGTNLSWRTPIAIIDGSYSHSKNAWQSGGSISSGLVVW
                     PGGINITNQLSDTFAILDAPGLEGAHINGQKYNRTNSKGQVVYDLIIPHRENHLVLDI
                     ANSESETELQGNRQIIAPYRGAVSYVQFTTDQRKPWYIQALRPDGSPLTFGYDVLDLQ
                     ENNIGVVGQGSRLFIRVDEIPTGIKVALNDEQNLFCTITFQHVIDENKTYICQ"
     gene            655..>896
                     /locus_tag="ACHOSC_RS26325"
                     /old_locus_tag="pgaptmp_005265"
     CDS             655..>896
                     /locus_tag="ACHOSC_RS26325"
                     /old_locus_tag="pgaptmp_005265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000871680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial family protein"
                     /protein_id="HGU4550988.1"
                     /translation="MKWLLLITLSLYSFIVQSAPCALTNVGEQRGTYILKPLSMKGNL
                     TAEKIENTGNIIECTDLQETTNAIKVHFLTQNALRF"
BASE COUNT          288 a          176 c          182 g          250 t
ORIGIN      
        1 caaaataatg atacctcgct tggtacgaat ttaagctgga gaactcccat cgccataata
       61 gatggcagct atagtcattc taaaaacgcc tggcaaagtg gtggaagtat tagtagtgga
      121 ttagttgtct ggcccggtgg tattaatatc actaaccagt tatccgatac atttgcaatt
      181 ctggatgcgc ctgggttaga aggtgcacat attaatggac aaaaatacaa tcgaacaaac
      241 agcaaaggcc aggttgttta cgacctgata atacctcatc gtgaaaacca tctggtactt
      301 gatatagcaa acagtgaaag tgaaacagaa ttgcagggca atcgtcaaat tattgcacct
      361 taccgtggag cagtttctta tgtgcagttt acaactgacc aacgtaagcc ctggtatata
      421 caggcactgc gtcccgatgg ttcgccatta acctttggct atgacgtact ggatctccag
      481 gaaaataata ttggagtcgt tggccagggt agtcgccttt ttattcgcgt agatgaaatt
      541 cccactggca taaaagttgc tctcaatgat gaacagaact tattctgtac tattactttt
      601 caacacgtta tcgatgaaaa caaaacatat atatgccagt aaaaggagtt tactatgaag
      661 tggcttttat taatcacctt gagtctatat tcttttattg tgcaaagtgc tccctgtgca
      721 ttgaccaatg tcggtgaaca acgtggaaca tatatactca agccactgag tatgaaaggt
      781 aatctaaccg ccgaaaaaat tgaaaacacc ggaaatatca ttgagtgtac agacttgcaa
      841 gagacaacca acgccattaa agttcatttt ttaacacaga acgctttaag gtttaa
//
LOCUS       DAZKHF020000618          896 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2201S31241
            SAMN43267800-rid25106413.denovo.622, whole genome shotgun sequence.
ACCESSION   DAZKHF020000618 DAZKHF020000000
VERSION     DAZKHF020000618.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267800
            Sequence Read Archive: SRR30307263
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 896)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 896)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 896)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350581.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350581.2
            Genome Coverage       :: 151x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:21:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,652
            CDSs (total)                      :: 5,557
            Genes (coding)                    :: 5,320
            CDSs (with protein)               :: 5,320
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 237
            CDSs (without protein)            :: 237
            Pseudo Genes (ambiguous residues) :: 0 of 237
            Pseudo Genes (frameshifted)       :: 102 of 237
            Pseudo Genes (incomplete)         :: 130 of 237
            Pseudo Genes (internal stop)      :: 52 of 237
            Pseudo Genes (multiple problems)  :: 41 of 237
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..896
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267800-rid25106413.denovo.622"
                     /serotype="O 26:H11"
                     /isolate="2201S31241"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-01"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            <1..344
                     /locus_tag="ACHOSC_RS26330"
                     /old_locus_tag="pgaptmp_005266"
     CDS             <1..344
                     /locus_tag="ACHOSC_RS26330"
                     /old_locus_tag="pgaptmp_005266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309145.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="tail assembly protein"
                     /protein_id="HGU4550989.1"
                     /translation="SFFTAGASMALWGSALAAGGFSATTMLFSLGASMILGGVAQMLA
                     PKPKTPEYRATDNGRQNTYFSSLDNMIAQGNPMPVPYGEMLVGSRRISQDISTRDEGG
                     DGKMVVIGRQA"
     gene            583..>896
                     /locus_tag="ACHOSC_RS26335"
                     /old_locus_tag="pgaptmp_005267"
     CDS             583..>896
                     /locus_tag="ACHOSC_RS26335"
                     /old_locus_tag="pgaptmp_005267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4550990.1"
                     /translation="MGKGGGKAHTPVEAKDNLKSTQMMSVIDAIGEGPIEGPVKGLQS
                     ILVNKTPLTDTDGNPVIHGVTAVWRAGEQEQTPPEGFESSGAETGLGVEVTKAKPVTR
                     TIT"
BASE COUNT          231 a          208 c          279 g          178 t
ORIGIN      
        1 ggtcgttctt cacggccggg gcatcaatgg cgttatgggg ttcagccctg gcagccggtg
       61 gtttttctgc caccacgatg ctgttttcac tgggggccag catgatactg ggcggtgtgg
      121 cccagatgct tgccccgaag ccaaaaacac cggaatacag ggcaacggat aacggcagac
      181 agaacacgta cttttcctcg ctggataaca tgattgccca ggggaacccg atgccggtgc
      241 cttacggtga aatgctggtt ggctcccggc gaatctccca ggacatcagt acccgtgatg
      301 aaggcggtga cgggaagatg gtggttatcg ggcggcaggc ataaaagcga aaaaatcccg
      361 cagtgctcac ggacaggaac tgcgggagcg ttacgaagat tgagtgtaag gaattattct
      421 tatgtcacga caaaaaacat taacgcagag aaattattag cgccacagtc agtttgtgaa
      481 aatgtgaaga tattcagaat ttttattcag tcatgataca ggcatcctcc gggatgcctg
      541 ttgtttttgt gcgtaacagt tatcacagta aagggtgaga caatgggcaa aggtggcggc
      601 aaggcgcaca cgccggtaga ggcaaaggac aatcttaagt ccacgcagat gatgagcgtg
      661 attgatgcca ttggtgaagg gccgattgaa ggcccggtga aggggctgca gagtatcctg
      721 gtgaacaaaa ccccgctgac ggacacggac ggcaatcctg tgatacacgg tgtgaccgcg
      781 gtctggcgcg ccggggagca ggagcagaca ccaccggaag gctttgagtc ctccggagct
      841 gaaaccggac tgggcgtgga agtgacgaag gcaaagccgg taacgcgcac cattac
//
LOCUS       DAZKHF020000619          895 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2201S31241
            SAMN43267800-rid25106413.denovo.623, whole genome shotgun sequence.
ACCESSION   DAZKHF020000619 DAZKHF020000000
VERSION     DAZKHF020000619.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267800
            Sequence Read Archive: SRR30307263
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 895)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 895)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 895)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350581.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350581.2
            Genome Coverage       :: 151x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:21:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,652
            CDSs (total)                      :: 5,557
            Genes (coding)                    :: 5,320
            CDSs (with protein)               :: 5,320
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 237
            CDSs (without protein)            :: 237
            Pseudo Genes (ambiguous residues) :: 0 of 237
            Pseudo Genes (frameshifted)       :: 102 of 237
            Pseudo Genes (incomplete)         :: 130 of 237
            Pseudo Genes (internal stop)      :: 52 of 237
            Pseudo Genes (multiple problems)  :: 41 of 237
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..895
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267800-rid25106413.denovo.623"
                     /serotype="O 26:H11"
                     /isolate="2201S31241"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-01"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            complement(<1..153)
                     /locus_tag="ACHOSC_RS26340"
                     /old_locus_tag="pgaptmp_005268"
     CDS             complement(<1..153)
                     /locus_tag="ACHOSC_RS26340"
                     /old_locus_tag="pgaptmp_005268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S49 family peptidase"
                     /protein_id="HGU4550991.1"
                     /translation="MRRNLSHIIAAAFNEPLLLEPAYARVFFCALGREMGASSLSVPQ
                     QQVQFDA"
     gene            complement(143..>895)
                     /locus_tag="ACHOSC_RS26345"
                     /old_locus_tag="pgaptmp_005269"
     CDS             complement(143..>895)
                     /locus_tag="ACHOSC_RS26345"
                     /old_locus_tag="pgaptmp_005269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HGU4550992.1"
                     /translation="SELDTEKAFEYIAGAPQGQKDNPLINILDKFSTWYDTNNVTLGG
                     VKIPHLFPGDDLKLQTAQDSDNGFSALEQALLRYIAAGLGVSYEQLSRDYSKVSYSSA
                     RASANESWRYFMGRRKFIASRLATQMFSCWLEEALLRGIIRPPRARFDFYQARSAWSR
                     AEWIGAGRMAIDGLKEVQESVMRIEAGLSTYEKELALMGEDYQDIFRQQVRESAERQK
                     AGLSRPVWIAQAYEQQIAESRRPEEETTPRET"
BASE COUNT          207 a          270 c          217 g          201 t
ORIGIN      
        1 agcatcaaac tgtacctgct gttgtggtac cgaaagactt gatgccccca tctcgcgccc
       61 gagcgcgcaa aagaaaaccc gcgcataggc gggctccaga agcagcggtt cattgaatgc
      121 tgcggcaata atgtgtgaaa gattacgtct cacgtggtgt tgtctcctct tccggcctgc
      181 gactctccgc tatctgctgc tcatacgcct gcgctatcca caccggacgt gagagtccgg
      241 ctttttgtcg ctcagcagat tccctgacct gctggcggaa aatgtcctga taatcctcgc
      301 ccatcagcgc cagctctttc tcatacgtgc tcagtccggc ctcaatgcgc atcactgatt
      361 cctggacttc cttgagcccg tcaatggcca ttcttccggc tccaatccac tcagcccgtg
      421 accaggctga tcgcgcctga taaaaatcaa aacgcgcccg tggcggacga ataatccccc
      481 gaagaagtgc ctcttccagc cagcaggaaa acatctgcgt ggccagccgg gacgcaataa
      541 attttcgtcg tcccataaaa tagcgccacg actcattggc ggaggcgcgg gcacttgaat
      601 aactgacctt cgagtaatcc cgggacaact gttcgtagga aacgccaaga ccggcggcga
      661 tataccgcag cagcgcctgt tcaagcgccg aaaatccatt gtctgaatcc tgcgcagtct
      721 gcagtttcag atcatcaccg gggaaaaggt gcggaatttt gacaccgccc agcgtcacgt
      781 tattcgtgtc ataccaggtg gagaacttat ccagaatatt aataagcgga ttatccttct
      841 gcccctgcgg cgcaccggcg atatattcaa aggccttttc ggtatcaagt tcact
//
LOCUS       DAZKHF020000620          894 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2201S31241
            SAMN43267800-rid25106413.denovo.624, whole genome shotgun sequence.
ACCESSION   DAZKHF020000620 DAZKHF020000000
VERSION     DAZKHF020000620.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267800
            Sequence Read Archive: SRR30307263
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 894)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 894)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 894)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350581.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350581.2
            Genome Coverage       :: 151x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:21:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,652
            CDSs (total)                      :: 5,557
            Genes (coding)                    :: 5,320
            CDSs (with protein)               :: 5,320
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 237
            CDSs (without protein)            :: 237
            Pseudo Genes (ambiguous residues) :: 0 of 237
            Pseudo Genes (frameshifted)       :: 102 of 237
            Pseudo Genes (incomplete)         :: 130 of 237
            Pseudo Genes (internal stop)      :: 52 of 237
            Pseudo Genes (multiple problems)  :: 41 of 237
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..894
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267800-rid25106413.denovo.624"
                     /serotype="O 26:H11"
                     /isolate="2201S31241"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-01"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            430..894
                     /locus_tag="ACHOSC_RS26350"
                     /old_locus_tag="pgaptmp_005270"
                     /pseudo
     CDS             430..894
                     /locus_tag="ACHOSC_RS26350"
                     /old_locus_tag="pgaptmp_005270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001545887.1"
                     /note="frameshifted; internal stop; incomplete; missing
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
BASE COUNT          253 a          179 c          186 g          276 t
ORIGIN      
        1 acagcgtcat caccttattc agtcgacctt caaaataaac gtttaacaga gacaatcacc
       61 atttggattg tccgctgagg aggcgaacaa tttcctagac agctttatca tgtcagccca
      121 cattgttggc gctggcaata ctaaggacaa acgatgggga agtagctttt tagcgttatt
      181 ttcgggagta aaaaacgtgg tatctttccg tatgtcagtt gtatcttgct ggtattcgta
      241 gtcgtgataa ggcatacaac gatatttatc ttgacgttag tgaagaataa gttgttttgt
      301 gatctatgtt aatctgggat tacaatccgg gcttgataca tcgcatcacg acttttattc
      361 tcttcagaat agaatatgtt actccccgct ttacaggctc aggatttgaa ttacaaatta
      421 tacttttaag tgaacaataa ccaagggtat ttcttatttc ctgaaaccag gcttggccca
      481 tttcgggatg agtttgaaaa aactcttgac gcatacgata gtgatgaaat aaacgaaaca
      541 aattacgtca acaaactcag gcgtcttgcc tgacatgctc ctgacatcat tgatattcac
      601 gctcatctgg catatgcatt tgcgctcttt atgctgcaat tctggcgaat gttcatttac
      661 agcgtcatca ggatgccgtt atactcattg ataaaatact aacatataac cctgagaata
      721 atcagggaac acgctggtta ctgggggcgg aattgttacg tgctggcaag catggcaggg
      781 ctctgtacgt attgacagga catgtcaatg aatttccccc ctactggtac gagcttgctc
      841 ttttacattt tatgaatgag gcatatatta atgctgcaac ggcatttcga tatg
//
LOCUS       DAZKHF020000621          892 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2201S31241
            SAMN43267800-rid25106413.denovo.625, whole genome shotgun sequence.
ACCESSION   DAZKHF020000621 DAZKHF020000000
VERSION     DAZKHF020000621.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267800
            Sequence Read Archive: SRR30307263
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 892)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 892)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 892)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350581.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350581.2
            Genome Coverage       :: 151x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:21:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,652
            CDSs (total)                      :: 5,557
            Genes (coding)                    :: 5,320
            CDSs (with protein)               :: 5,320
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 237
            CDSs (without protein)            :: 237
            Pseudo Genes (ambiguous residues) :: 0 of 237
            Pseudo Genes (frameshifted)       :: 102 of 237
            Pseudo Genes (incomplete)         :: 130 of 237
            Pseudo Genes (internal stop)      :: 52 of 237
            Pseudo Genes (multiple problems)  :: 41 of 237
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..892
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267800-rid25106413.denovo.625"
                     /serotype="O 26:H11"
                     /isolate="2201S31241"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-01"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            complement(33..>892)
                     /locus_tag="ACHOSC_RS26355"
                     /old_locus_tag="pgaptmp_005271"
     CDS             complement(33..>892)
                     /locus_tag="ACHOSC_RS26355"
                     /old_locus_tag="pgaptmp_005271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="terminase TerL endonuclease subunit"
                     /protein_id="HGU4550993.1"
                     /translation="LLMENLAMVNPNLGYSVDEAFLYREYRKAREAGEETFRGFMSKH
                     ANVEIGLALRSDRWAGADFWEEQGRCISLDDILRRADVVTVGIDGGGLDDLLGMYVTG
                     RDRETREWLGWGHAWAHETAVVRRKSEASRFQDLVACGDMTIVRRVGDDTAEVAEYVR
                     RIHEAELLDHIGIDPSGVGQILDSLAEAGIPDGIVVGISQGWKLGGAIKTTERKLAEG
                     VLVHGDQPLMAWCVGNARVEPKGNAILITKQASGRGKIDPLMALFNAVSLMSLNPEPK
                     KKEYAVFFI"
BASE COUNT          190 a          303 c          211 g          188 t
ORIGIN      
        1 agcggttcgt gatggttaca gtgtgaacag ggttatatga aaaaaaccgc atattctttc
       61 tttttcggtt ccgggttaag ggacatcagg gagaccgcat tgaacagcgc catcagcggg
      121 tcaatttttc cccgtccact ggcctgtttg gtgataagaa tggcgttacc tttaggctcc
      181 acccgggcat tgccgacaca ccaggccatc aggggctggt caccatgcac cagcacccct
      241 tcagccagtt tgcgctcggt ggttttaatg gccccgccca gtttccagcc ctggcttatc
      301 cccaccacaa ttccgtcggg gatcccggct tccgccagtg aatccagaat ctgccccacc
      361 cctgacgggt caataccgat atggtccagt aactcagcct catgaatgcg acgcacatat
      421 tccgccactt ccgccgtgtc atccccgaca cgccggacaa tggtcatatc tccacaggca
      481 acaagatcct gaaaccggga cgcctcgctc ttccgtcgga ccaccgcggt ttcatgcgcc
      541 caggcatggc cccagcccag ccattcgcgg gtctcccggt cacgcccagt cacatacatc
      601 cccagcagat catccagccc tccgccgtca atccccaccg tcaccacatc agcacgacgc
      661 aggatatcgt ccaggctgat acaacggccc tgctcttccc agaaatcagc ccccgcccag
      721 cggtcagagc gcagggcaag accaatttcc acattggcgt gttttgacat gaacccccgg
      781 aatgtctctt caccggcttc ccgggcttta cggtactccc ggtacagaaa ggcctcatcc
      841 actgaatagc cgagattcgg attgaccatg gcgaggtttt ccatcagcag gt
//
LOCUS       DAZKHF020000622          889 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2201S31241
            SAMN43267800-rid25106413.denovo.626, whole genome shotgun sequence.
ACCESSION   DAZKHF020000622 DAZKHF020000000
VERSION     DAZKHF020000622.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267800
            Sequence Read Archive: SRR30307263
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 889)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 889)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 889)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350581.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350581.2
            Genome Coverage       :: 151x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:21:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,652
            CDSs (total)                      :: 5,557
            Genes (coding)                    :: 5,320
            CDSs (with protein)               :: 5,320
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 237
            CDSs (without protein)            :: 237
            Pseudo Genes (ambiguous residues) :: 0 of 237
            Pseudo Genes (frameshifted)       :: 102 of 237
            Pseudo Genes (incomplete)         :: 130 of 237
            Pseudo Genes (internal stop)      :: 52 of 237
            Pseudo Genes (multiple problems)  :: 41 of 237
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..889
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267800-rid25106413.denovo.626"
                     /serotype="O 26:H11"
                     /isolate="2201S31241"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-01"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            46..855
                     /locus_tag="ACHOSC_RS26360"
                     /old_locus_tag="pgaptmp_005272"
     CDS             46..855
                     /locus_tag="ACHOSC_RS26360"
                     /old_locus_tag="pgaptmp_005272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HGU4550994.1"
                     /translation="MYWIINDNIEFWPEHRKLISVHNADLNVVLTTPASRCLSLLLEA
                     FPDVVAQQDFFTRVWEEEGMRVPTNTLYQNISIIRRGFRAVGATTHSLIATVPRRGFK
                     IHNDINIQNHVINSSTDAHTHNAPPAIKVNAGYKESIGGAKNFNNKILKHIKSHLIML
                     SAFVIGAYSAYWLWNNNQPKPFFKDYKTVAEINGCHFNVTEDTIDGLKEFDKYKTRIL
                     DSGINCKKHPWLYIPLAKSSPGMIVMACNKNYNQHEVADCLTLSYREVNRD"
BASE COUNT          292 a          170 c          169 g          258 t
ORIGIN      
        1 aaactatgtg atctccattt cgattgattt agtgtttatt gacgtatgta ctggattatt
       61 aacgataata tcgagttctg gcctgagcac cgaaaattaa tatcggtaca taacgccgat
      121 cttaacgtcg ttctgacaac gccagccagt cgatgtttat cacttctact tgaagctttt
      181 cctgatgtgg ttgcacaaca agattttttc accagagtct gggaagaaga aggtatgcgt
      241 gtgcctacta acacgttata tcagaacata tccattatca gacgcggatt tcgcgctgtt
      301 ggtgctacta cccactcgct aattgcaacc gtgccgagaa gaggattcaa gatccataat
      361 gacattaaca tacaaaatca tgtaataaac tcgtcaacag acgcacatac acacaatgcc
      421 ccacctgcca taaaagttaa tgcgggatac aaagagagca ttggtggcgc aaagaatttc
      481 aataacaaaa tcctcaaaca tataaaaagt catctaatta tgttgagcgc atttgtcata
      541 ggcgcatatt ctgcatattg gctatggaat aataatcaac cgaagccatt tttcaaggat
      601 tacaagactg ttgcggaaat taatggctgc cattttaatg taacagaaga tacaattgac
      661 gggttgaaag agttcgataa atataaaaca cggatactgg attcggggat taactgtaaa
      721 aaacatccgt ggttatacat ccctcttgca aaatcctcac cggggatgat tgttatggca
      781 tgcaataaaa attataacca acatgaagtg gcagactgtt taaccctgtc ttaccgtgag
      841 gttaatcgtg attgattata aaaaaaactt attatttatt ttagttttt
//
LOCUS       DAZKHF020000623          889 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2201S31241
            SAMN43267800-rid25106413.denovo.627, whole genome shotgun sequence.
ACCESSION   DAZKHF020000623 DAZKHF020000000
VERSION     DAZKHF020000623.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267800
            Sequence Read Archive: SRR30307263
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 889)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 889)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 889)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350581.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350581.2
            Genome Coverage       :: 151x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:21:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,652
            CDSs (total)                      :: 5,557
            Genes (coding)                    :: 5,320
            CDSs (with protein)               :: 5,320
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 237
            CDSs (without protein)            :: 237
            Pseudo Genes (ambiguous residues) :: 0 of 237
            Pseudo Genes (frameshifted)       :: 102 of 237
            Pseudo Genes (incomplete)         :: 130 of 237
            Pseudo Genes (internal stop)      :: 52 of 237
            Pseudo Genes (multiple problems)  :: 41 of 237
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..889
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267800-rid25106413.denovo.627"
                     /serotype="O 26:H11"
                     /isolate="2201S31241"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-01"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            <1..>889
                     /locus_tag="ACHOSC_RS26365"
                     /old_locus_tag="pgaptmp_005273"
     CDS             <1..>889
                     /locus_tag="ACHOSC_RS26365"
                     /old_locus_tag="pgaptmp_005273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="RHS element protein"
                     /protein_id="HGU4550995.1"
                     /translation="VYDRSGKQVRCFTYDDKYRGRMVAHRHTGRPEICYRYDSDGRVT
                     EQLNPAGLSYTYQYEKDRITITDSLNRREVLHTQGEAGLKRVVKKEHADGSVTQSQFD
                     AVGRLKAQTDAAGRTTEYSPDVVTGLTTPDGRASAFYYNHHNQLTSATGPDGLELRRE
                     YDESGRLIQETAPDGDITRYRYDNPHSDLPCATEDATGSRKTMTWSRYGQLLSFTDCS
                     GYVTRYDHDRFGQVTAVHREEGLSQYHAYDSRGQLTAVKDTQGHETRYEYNIAGDLTA
                     VIAPDGSRNGTQYDAWGKAI"
BASE COUNT          225 a          248 c          272 g          144 t
ORIGIN      
        1 ggtgtatgac cgtagtggca aacaggtgcg ctgctttact tacgatgata aataccgggg
       61 ccggatggtg gcgcaccgtc acacgggccg accggaaatc tgttaccgtt acgacagcga
      121 cgggcgggtg acagaacagc taaacccggc aggcttaagt tacacgtatc agtatgaaaa
      181 agaccgcatc accatcaccg acagcctgaa ccgccgtgaa gtgctgcaca cgcagggcga
      241 agccgggctg aagcgggtgg tgaaaaagga acacgcggac ggcagcgtca cgcagagtca
      301 gtttgacgcg gtgggcaggc ttaaggcaca gacggatgcc gcaggccgga caacagaata
      361 cagcccggat gtggtgacgg gcctcaccac gccggatggc agggcatcgg cgttttacta
      421 taaccaccac aaccagttaa cgtcagccac cgggcctgac gggctggaat tgcgccggga
      481 atatgatgaa tccggccgtc tgattcagga aactgcccct gacggcgata tcacccgcta
      541 ccgttatgat aatccacaca gtgacttacc ctgcgcaacg gaagatgcca ccggcagccg
      601 gaaaaccatg acgtggagcc gttacggtca gttgctgagc ttcaccgact gttccggtta
      661 tgtaacccgc tatgaccatg accgttttgg tcaggtgacg gcggtgcacc gcgaggaagg
      721 cctgagccag taccacgcat acgacagccg tggacagtta actgcggtga aagacacgca
      781 gggccatgaa acgcggtatg aatacaacat cgccggtgac ctgaccgccg tcattgcccc
      841 ggacggcagc agaaacggga cacagtacga tgcgtgggga aaggccatc
//
LOCUS       DAZKHF020000624          888 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2201S31241
            SAMN43267800-rid25106413.denovo.628, whole genome shotgun sequence.
ACCESSION   DAZKHF020000624 DAZKHF020000000
VERSION     DAZKHF020000624.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267800
            Sequence Read Archive: SRR30307263
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 888)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 888)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 888)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350581.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350581.2
            Genome Coverage       :: 151x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:21:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,652
            CDSs (total)                      :: 5,557
            Genes (coding)                    :: 5,320
            CDSs (with protein)               :: 5,320
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 237
            CDSs (without protein)            :: 237
            Pseudo Genes (ambiguous residues) :: 0 of 237
            Pseudo Genes (frameshifted)       :: 102 of 237
            Pseudo Genes (incomplete)         :: 130 of 237
            Pseudo Genes (internal stop)      :: 52 of 237
            Pseudo Genes (multiple problems)  :: 41 of 237
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..888
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267800-rid25106413.denovo.628"
                     /serotype="O 26:H11"
                     /isolate="2201S31241"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-01"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            <1..>888
                     /locus_tag="ACHOSC_RS26370"
                     /old_locus_tag="pgaptmp_005274"
     CDS             <1..>888
                     /locus_tag="ACHOSC_RS26370"
                     /old_locus_tag="pgaptmp_005274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sialate O-acetylesterase"
                     /protein_id="HGU4550996.1"
                     /translation="LHIAKKLLPYIPNNAGILLAPCCRGGSAFTQGAEGTFSADTGAS
                     QDSARWGVGKPLYQDLIARTKAALQKNPKNVLLAVCWMQGEFDMSAATYAQQPALFTA
                     MLKQFRADLTVFNAQCHGGSAVNVPWICGDTTYYWKNTYATQYDTVYGGYKNRESEGV
                     YFVPFMTDGNGVNTATNAPAEDPDIPASGYYGAASRTNGNQVSSNRPTHFSSWARRSI
                     IPDRMATAILNAAGRTSAFISGKAPEIKPSPGGDTPSGPSADTSVRTISLLPTAGEAA
                     AQGWTIKDGGIQLSGGVFKI"
BASE COUNT          210 a          230 c          260 g          188 t
ORIGIN      
        1 ttacatattg ccaaaaaact gcttccgtat atcccgaata acgcggggat cctgctggca
       61 ccatgctgtc gtggtggttc ggcattcacc cagggcgcgg aggggacatt cagtgcggac
      121 acgggggcca gccaggattc ggcacgctgg ggtgtgggta aaccgttata tcaggacctg
      181 attgcgcgca ctaaagctgc attacagaag aacccgaaaa atgtgttgct ggcggtgtgc
      241 tggatgcagg gagagtttga catgagcgcc gccacctacg cacagcaacc tgcgctgttt
      301 acagccatgc tgaagcagtt tcgtgctgac ctcactgtgt ttaacgcgca gtgtcatggt
      361 ggcagtgctg taaatgtgcc gtggatttgt ggtgacacga cgtattactg gaaaaataca
      421 tacgctaccc agtacgacac cgtgtacggc gggtataaaa acagggagag tgagggcgtt
      481 tattttgtgc ccttcatgac agacggtaac ggcgtcaata ccgccactaa cgcaccggca
      541 gaagatccgg atattccggc atcaggatat tacggtgcgg catcgagaac gaatggaaac
      601 caggtatcat caaaccgccc gacacatttc agttcatggg cgcgcaggag cattattccg
      661 gatcgtatgg caaccgctat tctgaacgca gccgggcgca cctcagcttt catcagtggt
      721 aaggcaccgg aaatcaaacc ctcgcccggc ggcgacacgc catcgggtcc gtctgcagat
      781 acgtccgttc gcacaatctc cctgctgccg acagccggag aggctgctgc gcagggctgg
      841 accattaagg acggcggaat tcagttgtcg ggtggtgtat ttaagatc
//
LOCUS       DAZKHF020000625          884 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2201S31241
            SAMN43267800-rid25106413.denovo.629, whole genome shotgun sequence.
ACCESSION   DAZKHF020000625 DAZKHF020000000
VERSION     DAZKHF020000625.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267800
            Sequence Read Archive: SRR30307263
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 884)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 884)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 884)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350581.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350581.2
            Genome Coverage       :: 151x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:21:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,652
            CDSs (total)                      :: 5,557
            Genes (coding)                    :: 5,320
            CDSs (with protein)               :: 5,320
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 237
            CDSs (without protein)            :: 237
            Pseudo Genes (ambiguous residues) :: 0 of 237
            Pseudo Genes (frameshifted)       :: 102 of 237
            Pseudo Genes (incomplete)         :: 130 of 237
            Pseudo Genes (internal stop)      :: 52 of 237
            Pseudo Genes (multiple problems)  :: 41 of 237
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..884
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267800-rid25106413.denovo.629"
                     /serotype="O 26:H11"
                     /isolate="2201S31241"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-01"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            <1..>884
                     /locus_tag="ACHOSC_RS26375"
                     /old_locus_tag="pgaptmp_005275"
     CDS             <1..>884
                     /locus_tag="ACHOSC_RS26375"
                     /old_locus_tag="pgaptmp_005275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4550997.1"
                     /translation="PLHDEIGINFKDLPGPLKNALSNSNIPTTVSTAASTIASATTST
                     VTTASKDPIPWFGLTAQVVRNHGVELPIVKTENGWKLVGETPLTPDGPKANYTEEWVI
                     RPGEADFKYGASPLQATLGLEFGAHFKWDLDNPNTKYAVLTNAAANALGAVGGFAVSR
                     FTGTDPMLSPHIGAMVGQAAGHAIQYNTPGLKPDTILWWAGTTLGLADLNKAETGEGR
                     FTDYPRIWWHAREGAIFPNKEDIARATGADIRAMEEGIPVGQRHPNPEDVVIDIESNG
                     SPHHNPSNYVDTFDIIQE"
BASE COUNT          269 a          193 c          201 g          221 t
ORIGIN      
        1 tccctttgca tgatgaaata ggtattaatt ttaaagacct tcctggtcca ttaaaaaatg
       61 cattaagcaa cagcaatata ccaaccactg tatcgactgc tgcatccact attgcatcag
      121 ccactacttc gacggtaacc accgcgtcaa aagacccaat accatggttt ggattaacag
      181 ctcaagtagt tcgtaatcat ggtgtggaac ttcctatagt caaaactgaa aatggatgga
      241 agcttgttgg agaaactcct cttactcctg atggccccaa agcaaattat actgaagagt
      301 gggtgatcag accgggagaa gcagatttta aatatggtgc atctccacta caggcaactc
      361 tagggctgga gtttggcgca catttcaagt gggatttaga taaccctaat actaaatatg
      421 ccgttcttac caatgctgcc gcaaatgcgc ttggtgctgt agggggattt gcagtatcca
      481 gatttactgg tacagatcca atgttaagtc ctcatatcgg tgcaatggtt gggcaagcag
      541 cggggcatgc catacagtat aatacccccg gattaaagcc agacactatt ttatggtggg
      601 caggtacgac tctggggctg gcagatttaa acaaagccga gacaggtgaa ggcagattca
      661 ctgactatcc tcgtatatgg tggcacgcaa gagaaggtgc catcttccct aataaagaag
      721 atattgcccg tgcaacaggg gctgatatac gagctatgga agaaggtata cccgttggac
      781 agcgacatcc aaatccagag gatgtggtca tcgatatcga aagcaatggc tcaccacatc
      841 ataacccatc aaattatgtt gatacctttg atataatcca agaa
//
LOCUS       DAZKHF020000626          883 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2201S31241
            SAMN43267800-rid25106413.denovo.630, whole genome shotgun sequence.
ACCESSION   DAZKHF020000626 DAZKHF020000000
VERSION     DAZKHF020000626.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267800
            Sequence Read Archive: SRR30307263
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 883)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 883)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 883)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350581.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350581.2
            Genome Coverage       :: 151x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:21:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,652
            CDSs (total)                      :: 5,557
            Genes (coding)                    :: 5,320
            CDSs (with protein)               :: 5,320
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 237
            CDSs (without protein)            :: 237
            Pseudo Genes (ambiguous residues) :: 0 of 237
            Pseudo Genes (frameshifted)       :: 102 of 237
            Pseudo Genes (incomplete)         :: 130 of 237
            Pseudo Genes (internal stop)      :: 52 of 237
            Pseudo Genes (multiple problems)  :: 41 of 237
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..883
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267800-rid25106413.denovo.630"
                     /serotype="O 26:H11"
                     /isolate="2201S31241"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-01"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            191..>883
                     /gene="cif"
                     /locus_tag="ACHOSC_RS26380"
                     /old_locus_tag="pgaptmp_005276"
     CDS             191..>883
                     /gene="cif"
                     /locus_tag="ACHOSC_RS26380"
                     /old_locus_tag="pgaptmp_005276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000652081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system effector Cif"
                     /protein_id="HGU4550998.1"
                     /translation="MKDITLPPPTSASCLTGAISVNTEAVLSPMQHTSALHVRDFASL
                     CSQNLKANVLLNSDDHEVPIHQKNPAAIMQNIDSNIKQMATDWGMSIEEVEVIIGREK
                     GIVEPSCGVTANAIMKLFLDKDGFSYCFENEQTLSLEQLQERLSCMPECKSFVLRVND
                     GALGHAYIVDIPKGENSCRPAFLYQSDLGEGVTRKLRFEDWMTHKALTPILLDDICNY
                     FSCMSQNKTDLEQ"
BASE COUNT          259 a          182 c          207 g          235 t
ORIGIN      
        1 gatggaggtg gtaaaaagtg gtgtggctgg caatccaggc cgcgtcacag aaatggacaa
       61 tgccgctgag agactggcga atggcataca ggcaccagaa aaatttcaga catgacataa
      121 tgcccatgcc agaagttctt tttgcacctc gcctgtattt atagataaat atagagtgaa
      181 tgaatgagat atgaaagaca ttacccttcc ccccccgacg tccgcgtcct gtctgacagg
      241 ggccatatct gtaaatactg aagctgtatt atctcccatg caacacactt cagccttaca
      301 tgtaagagat tttgcttccc tgtgctcaca gaacctcaaa gctaatgtat tgctaaatag
      361 tgatgaccac gaagtaccta tacatcagaa aaatcctgct gcaataatgc aaaatatcga
      421 ctctaacatc aaacagatgg caacagactg ggggatgtcg attgaggagg ttgaggttat
      481 tatagggcga gagaaaggca ttgtggaacc ctcctgcgga gttaccgcta atgctattat
      541 gaaactattt ctggacaagg atggcttcag ttactgcttt gaaaatgaac agacactatc
      601 gctcgagcag cttcaggagc gcctgtcctg tatgcctgaa tgtaagagct ttgtattacg
      661 tgttaatgat ggtgcgcttg gtcatgctta cattgtcgat attcccaaag gagaaaactc
      721 ttgtcgtcct gcattcttgt atcagtcaga tttaggagag ggcgtcacca gaaagttaag
      781 atttgaggac tggatgacgc ataaagcatt gactccgatt ttgctggatg atatttgtaa
      841 ttacttctcc tgcatgtctc aaaataagac agatttggag cag
//
LOCUS       DAZKHF020000627          883 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2201S31241
            SAMN43267800-rid25106413.denovo.631, whole genome shotgun sequence.
ACCESSION   DAZKHF020000627 DAZKHF020000000
VERSION     DAZKHF020000627.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267800
            Sequence Read Archive: SRR30307263
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 883)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 883)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 883)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350581.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350581.2
            Genome Coverage       :: 151x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:21:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,652
            CDSs (total)                      :: 5,557
            Genes (coding)                    :: 5,320
            CDSs (with protein)               :: 5,320
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 237
            CDSs (without protein)            :: 237
            Pseudo Genes (ambiguous residues) :: 0 of 237
            Pseudo Genes (frameshifted)       :: 102 of 237
            Pseudo Genes (incomplete)         :: 130 of 237
            Pseudo Genes (internal stop)      :: 52 of 237
            Pseudo Genes (multiple problems)  :: 41 of 237
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..883
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267800-rid25106413.denovo.631"
                     /serotype="O 26:H11"
                     /isolate="2201S31241"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-01"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            <1..>883
                     /locus_tag="ACHOSC_RS26385"
                     /old_locus_tag="pgaptmp_005277"
     rRNA            <1..>883
                     /locus_tag="ACHOSC_RS26385"
                     /old_locus_tag="pgaptmp_005277"
                     /product="23S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF02541"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF02541"
BASE COUNT          245 a          195 c          276 g          167 t
ORIGIN      
        1 ggaaaggcgc gcgatacagg gtgacagccc cgtacacaaa aatgcacatg ctgtgagctc
       61 gatgagtagg gcgggacacg tggtatcctg tctgaatatg gggggaccat cctccaaggc
      121 taaatactcc tgactgaccg atagtgaacc agtaccgtga gggaaaggcg aaaagaaccc
      181 cggcgagggg agtgaaaaag aacctgaaac cgtgtacgta caagcagtgg gagcacgctt
      241 aggcgtgtga ctgcgtacct tttgtataat gggtcagcga cttatattct gtagcaaggt
      301 taaccgaata ggggagccga agggaaaccg agtcttaact gggcgttaag ttgcagggta
      361 tagacccgaa acccggtgat ctagccatgg gcaggttgaa ggttgggtaa cactaactgg
      421 aggaccgaac cgactaatgt tgaaaaatta gcggatgact tgtggctggg ggtgaaaggc
      481 caatcaaacc gggagatagc tggttctccc cgaaagctat ttaggtagcg cctcgtgaat
      541 tcatctccgg gggtagagca ctgtttcggc aagggggtca tcccgactta ccaacccgat
      601 gcaaactgcg aataccggag aatgttatca cgggagacac acggcgggtg ctaacgtccg
      661 tcgtgaagag ggaaacaacc cagaccgcca gctaaggtcc caaagtcatg gttaagtggg
      721 aaacgatgtg ggaaggccca gacagccagg atgttggctt agaagcagcc atcatttaaa
      781 gaaagcgtaa tagctcactg gtcgagtcgg cctgcgcgga agatgtaacg gggctaaacc
      841 atgcaccgaa gctgcggcag cgacgctgat gcgttgttgg gta
//
LOCUS       DAZKHF020000628          879 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2201S31241
            SAMN43267800-rid25106413.denovo.632, whole genome shotgun sequence.
ACCESSION   DAZKHF020000628 DAZKHF020000000
VERSION     DAZKHF020000628.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267800
            Sequence Read Archive: SRR30307263
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 879)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 879)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 879)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350581.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350581.2
            Genome Coverage       :: 151x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:21:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,652
            CDSs (total)                      :: 5,557
            Genes (coding)                    :: 5,320
            CDSs (with protein)               :: 5,320
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 237
            CDSs (without protein)            :: 237
            Pseudo Genes (ambiguous residues) :: 0 of 237
            Pseudo Genes (frameshifted)       :: 102 of 237
            Pseudo Genes (incomplete)         :: 130 of 237
            Pseudo Genes (internal stop)      :: 52 of 237
            Pseudo Genes (multiple problems)  :: 41 of 237
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..879
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267800-rid25106413.denovo.632"
                     /serotype="O 26:H11"
                     /isolate="2201S31241"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-01"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            42..575
                     /locus_tag="ACHOSC_RS26390"
                     /old_locus_tag="pgaptmp_005278"
     CDS             42..575
                     /locus_tag="ACHOSC_RS26390"
                     /old_locus_tag="pgaptmp_005278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysozyme"
                     /protein_id="HGU4550999.1"
                     /translation="MNAKIRYGLSAAVLALIGAGASAPEILDQFLDEKEGNHTTAYRD
                     GAGIWTICRGAILVDGKPVVPGMKLSKEKCDQVNAIERDKALAWVEKNIRVPLTEPQK
                     AGIASFCPYNIGPGKCFPSTFYRRINAGDRRGACEAIRWWIKDGGRDCRIRSNNCYGQ
                     VSRRDQESALACWGIDR"
     gene            735..872
                     /locus_tag="ACHOSC_RS26395"
                     /old_locus_tag="pgaptmp_005279"
     CDS             735..872
                     /locus_tag="ACHOSC_RS26395"
                     /old_locus_tag="pgaptmp_005279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000459350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4551000.1"
                     /translation="MFDFSKLIREIRMMAEKLSTWKFILIWLVFVIMASGYFIGQIRW
                     W"
BASE COUNT          232 a          189 c          252 g          206 t
ORIGIN      
        1 atatccaggg atttgaaatc gataaaccct gataaatatc catgaacgca aaaatcagat
       61 acggcctgtc ggctgccgtt ctggcgctga ttggtgcagg ggcgtctgcg cctgaaatcc
      121 tcgaccagtt tcttgacgaa aaagaaggta accacaccac ggcataccgt gatggtgcgg
      181 ggatctggac catctgccgc ggtgccatcc tggtggatgg taaacctgtc gtcccgggca
      241 tgaagttgtc gaaggaaaaa tgcgaccagg ttaacgccat tgaacgtgat aaggcgctgg
      301 catgggtgga gaaaaacatc agagtgccac tgaccgaacc ccagaaagcg gggattgcgt
      361 cattctgtcc gtacaacatt ggccccggta agtgtttccc gtcgacgttt tacagacgga
      421 ttaatgccgg tgaccgcagg ggagcatgcg aggcgattcg ctggtggatt aaggacggtg
      481 gcagagactg ccgtattcgc tcaaacaact gctacggtca ggtctcacgg cgtgaccagg
      541 agagcgcgct ggcgtgctgg ggaattgaca gataagcaga atattttgct gaaaaatgcg
      601 atttgctcac gcggacggat aacacgaaat cctgcgaact gacaaaaact aagtgaataa
      661 aagtaaaaac cccgtttgtt ggctgcaagc ggggttttgt gtttcctgac tccggaaaag
      721 tcaaaggaga aagtgtgttt gattttagca aactgattcg ggagattcga atgatggctg
      781 aaaaattatc cacctggaag ttcatcctta tctggctggt gtttgtgatt atggcttccg
      841 gttatttcat tggtcagata cgctggtggt gaaatgaac
//
LOCUS       DAZKHF020000629          869 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2201S31241
            SAMN43267800-rid25106413.denovo.633, whole genome shotgun sequence.
ACCESSION   DAZKHF020000629 DAZKHF020000000
VERSION     DAZKHF020000629.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267800
            Sequence Read Archive: SRR30307263
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 869)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 869)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 869)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350581.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350581.2
            Genome Coverage       :: 151x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:21:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,652
            CDSs (total)                      :: 5,557
            Genes (coding)                    :: 5,320
            CDSs (with protein)               :: 5,320
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 237
            CDSs (without protein)            :: 237
            Pseudo Genes (ambiguous residues) :: 0 of 237
            Pseudo Genes (frameshifted)       :: 102 of 237
            Pseudo Genes (incomplete)         :: 130 of 237
            Pseudo Genes (internal stop)      :: 52 of 237
            Pseudo Genes (multiple problems)  :: 41 of 237
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..869
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267800-rid25106413.denovo.633"
                     /serotype="O 26:H11"
                     /isolate="2201S31241"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-01"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            complement(<1..181)
                     /gene="hha"
                     /locus_tag="ACHOSC_RS26400"
                     /old_locus_tag="pgaptmp_005280"
     CDS             complement(<1..181)
                     /gene="hha"
                     /locus_tag="ACHOSC_RS26400"
                     /old_locus_tag="pgaptmp_005280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin expression modulator Hha"
                     /protein_id="HGU4551001.1"
                     /translation="MSEKPLTKTDYLMRLRRCQTIDTLERVIEKNKYELSDNELAVFY
                     SAADHRLAELTMNKLY"
     gene            complement(207..581)
                     /gene="tomB"
                     /locus_tag="ACHOSC_RS26405"
                     /old_locus_tag="pgaptmp_005281"
     CDS             complement(207..581)
                     /gene="tomB"
                     /locus_tag="ACHOSC_RS26405"
                     /old_locus_tag="pgaptmp_005281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001741190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hha toxicity modulator TomB"
                     /protein_id="HGU4551002.1"
                     /translation="MDEYSPKRHDIAQLKFLCETLYHDCLANLEESNHGWVNDPTSAI
                     NLQLNELIEHIATFALNYKIKYNEDNKLIEQIDEYLDDTFMLFSSYGINMQDLQKWRK
                     SGNRLFRCFVNATKENPASLSC"
BASE COUNT          243 a          182 c          173 g          271 t
ORIGIN      
        1 cgtatagttt attcatggtc aattcggcga ggcggtgatc tgcggctgag taaaataccg
       61 ccagttcatt atctgataat tcgtatttat ttttctcgat aacacgctcc agcgtgtcaa
      121 ttgtctggca acgacgtaaa cgcattaaat aatcggtttt cgttaaaggt ttttcggaca
      181 tacttctacc catggttgta ataattctaa caagataaac tcgcaggatt ctctttcgtc
      241 gcattgacaa aacaacggaa tagtcgatta cctgacttcc gccatttctg aagatcctgc
      301 atattaatac cataactgct gaacaacata aaggtgtcat ccagatattc gtcgatctgc
      361 tcaatgagct tattgtcttc attatactta attttgtaat taagtgcgaa ggtcgcaata
      421 tgctcaatca gttcattcaa ctggaggttg atcgccgagg ttgggtcgtt aacccagcca
      481 tgattgcttt cttcaaggtt tgcaagacag tcatgataca gggtttcaca gagaaactta
      541 agctgtgcga tatcatgtct tttgggtgag tattcatcca taacgcgtcc ccttcttagt
      601 ggttgaacta acggacacct ttcgggatgg aaaaaactta ctgacctgga cttgccctct
      661 cgtttgttag cttaactata agccactctt tgcaggtttt catcgcatta ttacgaaaaa
      721 ttacaattag gaaggaatcg ggacaggact cactttttca tattagcact aacccgcagc
      781 aggtaaaagc agtttgctcg aactgttcat aacatttcag gattatcgcc agatgaaatt
      841 ggtgacacta ataccaggat tgctctgaa
//
LOCUS       DAZKHF020000630          865 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2201S31241
            SAMN43267800-rid25106413.denovo.634, whole genome shotgun sequence.
ACCESSION   DAZKHF020000630 DAZKHF020000000
VERSION     DAZKHF020000630.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267800
            Sequence Read Archive: SRR30307263
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 865)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 865)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 865)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350581.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350581.2
            Genome Coverage       :: 151x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:21:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,652
            CDSs (total)                      :: 5,557
            Genes (coding)                    :: 5,320
            CDSs (with protein)               :: 5,320
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 237
            CDSs (without protein)            :: 237
            Pseudo Genes (ambiguous residues) :: 0 of 237
            Pseudo Genes (frameshifted)       :: 102 of 237
            Pseudo Genes (incomplete)         :: 130 of 237
            Pseudo Genes (internal stop)      :: 52 of 237
            Pseudo Genes (multiple problems)  :: 41 of 237
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..865
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267800-rid25106413.denovo.634"
                     /serotype="O 26:H11"
                     /isolate="2201S31241"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-01"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            <1..748
                     /gene="gadX"
                     /locus_tag="ACHOSC_RS26410"
                     /old_locus_tag="pgaptmp_005282"
     CDS             <1..748
                     /gene="gadX"
                     /locus_tag="ACHOSC_RS26410"
                     /old_locus_tag="pgaptmp_005282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="acid resistance transcriptional activator GadX"
                     /protein_id="HGU4551003.1"
                     /translation="RYFNGGDLVFADASQIRVDKCVENFVLVSRDTLSLFLPMLKEEA
                     LNLHAHKKISSLLVHHCSRDIPVFQEVAQLSQNKNLRYAEMLRKRALIFALLSVFLED
                     EHFIPLLLNVLQPNMRTRVCTVINNNIAHEWTLARIASELLMSPSLLKKKLREEETSY
                     SQLLTECRMQRALQLIVIHGFSIKRVAVSCGYHSVSYFIYVFRNYYGMTPTEYQERSA
                     QGLPNRDSAASIVAQGNFYGTDRSAEGIRL"
BASE COUNT          245 a          169 c          199 g          252 t
ORIGIN      
        1 tcgctatttt aatggcggtg acctggtttt tgcggatgca agccaaattc gagtagataa
       61 gtgtgttgaa aattttgtat tagtatcaag ggatacgctt tcattatttc tgccgatgct
      121 caaggaggag gcattaaatc ttcatgcaca taaaaaaatt tcttcattac tcgttcatca
      181 ctgtagcaga gatattcctg tttttcagga agttgcgcaa ctatcgcaga ataagaatct
      241 tcgctatgca gaaatgctac gtaaaagagc attaatcttt gcgttgttat ctgtttttct
      301 tgaggatgag cactttatac cgctgctttt gaacgttttg caaccgaaca tgcgaacacg
      361 agtttgtacg gttatcaata ataatatcgc ccatgagtgg acactagccc gaatcgccag
      421 cgagctgttg atgagtccaa gcctgttaaa gaaaaaattg cgcgaagaag agacatcata
      481 ttcacagttg cttactgagt gtagaatgca acgtgctttg caacttattg ttatacatgg
      541 cttttcaatt aagcgagtcg cagtgtcctg tggatatcac agcgtgtcgt atttcattta
      601 cgtctttcga aattattatg ggatgacgcc cacagagtat caggagcgat cggcgcaggg
      661 attgccgaac cgtgactcgg cggcaagtat tgttgcgcaa ggaaattttt acggcactga
      721 ccgttctgcg gaaggaataa gattatagaa ttttactcag acataaaaaa aacccggcat
      781 aggggaccgg gaagaggata gtctgccgtc tccagactaa taaaccgtta taacactccc
      841 tgttggcacg ggaaactttg tgctc
//
LOCUS       DAZKHF020000631          862 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2201S31241
            SAMN43267800-rid25106413.denovo.635, whole genome shotgun sequence.
ACCESSION   DAZKHF020000631 DAZKHF020000000
VERSION     DAZKHF020000631.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267800
            Sequence Read Archive: SRR30307263
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 862)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 862)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 862)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350581.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350581.2
            Genome Coverage       :: 151x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:21:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,652
            CDSs (total)                      :: 5,557
            Genes (coding)                    :: 5,320
            CDSs (with protein)               :: 5,320
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 237
            CDSs (without protein)            :: 237
            Pseudo Genes (ambiguous residues) :: 0 of 237
            Pseudo Genes (frameshifted)       :: 102 of 237
            Pseudo Genes (incomplete)         :: 130 of 237
            Pseudo Genes (internal stop)      :: 52 of 237
            Pseudo Genes (multiple problems)  :: 41 of 237
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..862
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267800-rid25106413.denovo.635"
                     /serotype="O 26:H11"
                     /isolate="2201S31241"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-01"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            29..205
                     /locus_tag="ACHOSC_RS26415"
                     /old_locus_tag="pgaptmp_005283"
     CDS             29..205
                     /locus_tag="ACHOSC_RS26415"
                     /old_locus_tag="pgaptmp_005283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000753063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4551004.1"
                     /translation="MADFGSTKYSVSFEEWHELLMEYAELRGGSAADAEAWRDDYEAG
                     KNPVEAYCDEWGDE"
     gene            202..699
                     /locus_tag="ACHOSC_RS26420"
                     /old_locus_tag="pgaptmp_005284"
                     /pseudo
     CDS             202..699
                     /locus_tag="ACHOSC_RS26420"
                     /old_locus_tag="pgaptmp_005284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000185346.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ead/Ea22-like family protein"
BASE COUNT          266 a          146 c          244 g          206 t
ORIGIN      
        1 gaaataaaag acgaagagga agaacaaaat ggctgatttt ggttcaacta aatacagcgt
       61 cagttttgaa gaatggcatg aactgttaat ggaatatgca gagttacgtg gtggcagtgc
      121 tgctgatgct gaagcatggc gtgatgatta tgaagcagga aaaaatccgg tcgaagcata
      181 ttgtgatgag tggggcgatg aatgagcgag attgactatc aggcgctgcg tgaagcagca
      241 gagaaagcaa ctaaaggatg ctacatcgta gggcatacat cgggcaatca gcatgggaat
      301 ataacaggag tttttgtttg tcaaaaatgg aaaggagagc ccggtggcgt aattgcagaa
      361 tgtcatgtta actgcctggt tgaaacagat gttcaggctt atgcaaacgc tgaatttatt
      421 gctgctttta atccaaatgt tgcgctggca ctactggatg aacgggaagc ccaaaacaaa
      481 cgaattgcag agctgaaagc taatcttgtg gcgctggcgg cggagaatgc ggtaataaag
      541 tctgcaattc cagaaccacg ggatattgag gacgacaatg acaatatgga tgacgtatct
      601 cttgctgaag atttcgggtt caatcatgca atagaacgga tgaggagacg gattcctgaa
      661 actccggcca ccgacgcttt cctggctgaa gtacgggcgg ggttgtttaa cgagctttgc
      721 gcggcgtttg tcaggtacga aaaagttgca ggactggatg atagcgatac agtaacgctt
      781 aaggaagcga cagaagcatt gctggattgt gcggaacagc ttatcgcgcc tgaataatta
      841 aatttagtta aaaatttaat cc
//
LOCUS       DAZKHF020000632          857 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2201S31241
            SAMN43267800-rid25106413.denovo.636, whole genome shotgun sequence.
ACCESSION   DAZKHF020000632 DAZKHF020000000
VERSION     DAZKHF020000632.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267800
            Sequence Read Archive: SRR30307263
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 857)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 857)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 857)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350581.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350581.2
            Genome Coverage       :: 151x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:21:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,652
            CDSs (total)                      :: 5,557
            Genes (coding)                    :: 5,320
            CDSs (with protein)               :: 5,320
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 237
            CDSs (without protein)            :: 237
            Pseudo Genes (ambiguous residues) :: 0 of 237
            Pseudo Genes (frameshifted)       :: 102 of 237
            Pseudo Genes (incomplete)         :: 130 of 237
            Pseudo Genes (internal stop)      :: 52 of 237
            Pseudo Genes (multiple problems)  :: 41 of 237
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..857
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267800-rid25106413.denovo.636"
                     /serotype="O 26:H11"
                     /isolate="2201S31241"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-01"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            <1..458
                     /locus_tag="ACHOSC_RS26425"
                     /old_locus_tag="pgaptmp_005285"
     CDS             <1..458
                     /locus_tag="ACHOSC_RS26425"
                     /old_locus_tag="pgaptmp_005285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HGU4551005.1"
                     /translation="FESGDVRAYKIRFPNGTVDVFRGWVSSIGKAVTAKEVITRTVKV
                     TNVGKPSVAEERSEITPVTAIKVTPTSGTVAKGKTTTLTVSFEPESATDKTFRAVSAD
                     PSTGTIAVKDMAITVTGVKAGKVSIPVISGNGQFATVAEVTVTEAGAAG"
     gene            472..>857
                     /locus_tag="ACHOSC_RS26430"
                     /old_locus_tag="pgaptmp_005286"
     CDS             472..>857
                     /locus_tag="ACHOSC_RS26430"
                     /old_locus_tag="pgaptmp_005286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000479108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage minor tail protein G"
                     /protein_id="HGU4551006.1"
                     /translation="MFLKTEQFEYNGVSVTLSELSALQRIEHLALLKRRAEQAEASGN
                     LQVSVEDLVRTGAFLVAMSLWHNHPQKTESPSMNEAVMQIEQEVLTTWPADAIARAED
                     VVLRLSGMSGAVHVDTDITEVAKNNAL"
BASE COUNT          212 a          198 c          269 g          178 t
ORIGIN      
        1 ggtttgaaag cggggatgtg cgggcctata aaatccgttt cccgaacggc acggtggatg
       61 tgttccgtgg ctgggtcagc agtatcggta aggccgtaac ggcgaaggaa gtgatcaccc
      121 gtacggtgaa agtgaccaac gtgggtaaac cttctgtggc ggaagaacgc agcgaaatta
      181 cgccggtcac tgcgattaag gtgacgccga catctggtac ggtggcaaaa gggaaaacaa
      241 caaccctgac ggtttctttt gagccggaaa gtgcaaccga caaaacgttc agagcggttt
      301 ccgccgatcc gtcaacggga accattgctg tgaaagatat ggcgatcact gtgacggggg
      361 ttaaggctgg aaaagtgagt atccccgtga tttccggtaa tggtcagttt gccacggtag
      421 ctgaagtcac cgttactgaa gcgggcgctg cagggtaaac ggaggtaata catgtttctg
      481 aaaaccgaac aatttgaata taacggtgtg tccgtcacgc tttccgagct gtctgcgctg
      541 cagcgtatcg agcatcttgc cctgctgaaa cgacgggcag aacaggctga agccagcggt
      601 aacctgcagg tgagcgtgga agaccttgtc agaaccggcg cgtttctggt ggcgatgtcc
      661 ctgtggcata accatccaca gaaaacggag tcaccatcaa tgaatgaggc tgtgatgcag
      721 atcgaacagg aggtgctcac cacctggcct gctgatgcca ttgcccgggc ggaagacgtg
      781 gtgttgcgtc tgtccgggat gagcggggct gttcatgtgg atacggatat caccgaagtg
      841 gcgaaaaata acgcgct
//
LOCUS       DAZKHF020000633          856 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2201S31241
            SAMN43267800-rid25106413.denovo.637, whole genome shotgun sequence.
ACCESSION   DAZKHF020000633 DAZKHF020000000
VERSION     DAZKHF020000633.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267800
            Sequence Read Archive: SRR30307263
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 856)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 856)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 856)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350581.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350581.2
            Genome Coverage       :: 151x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:21:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,652
            CDSs (total)                      :: 5,557
            Genes (coding)                    :: 5,320
            CDSs (with protein)               :: 5,320
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 237
            CDSs (without protein)            :: 237
            Pseudo Genes (ambiguous residues) :: 0 of 237
            Pseudo Genes (frameshifted)       :: 102 of 237
            Pseudo Genes (incomplete)         :: 130 of 237
            Pseudo Genes (internal stop)      :: 52 of 237
            Pseudo Genes (multiple problems)  :: 41 of 237
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..856
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267800-rid25106413.denovo.637"
                     /serotype="O 26:H11"
                     /isolate="2201S31241"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-01"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            3..737
                     /gene="ydeO"
                     /locus_tag="ACHOSC_RS26435"
                     /old_locus_tag="pgaptmp_005287"
     CDS             3..737
                     /gene="ydeO"
                     /locus_tag="ACHOSC_RS26435"
                     /old_locus_tag="pgaptmp_005287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000060463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid stress response transcriptional regulator
                     YdeO"
                     /protein_id="HGU4551007.1"
                     /translation="MHHAFNANILDKDYAFSDGEILMVDNAVRTHFEPYERHFKEIGF
                     TENTIKKYLQCTNIQTVTVPVPAKFLRASNVPTGLLNEMIAYLNSEERNHHNFSELLL
                     FSCLSIFAACKGFITLLTNGVLSVSGKVRNIVNMKLAHPWKLKDICDCLYISESLLKK
                     KLKQEQTTFSQILLDARMQHAKNLIRVEGSVNKIAEQCGYASTSYFIYAFRKHFGNSP
                     KRVSKEYRCQSHTGMNTGNTMSALAI"
BASE COUNT          261 a          172 c          170 g          253 t
ORIGIN      
        1 ttattcatca tgccttcaac gctaacattt tagataaaga ttacgccttc tctgacggcg
       61 agatcctgat ggtagataac gctgttcgta cgcattttga accttatgag cggcatttta
      121 aagagatcgg atttactgaa aataccatta aaaaatatct acaatgcact aacatccaga
      181 cagtgacggt gcctgttcct gcgaagtttt tacgtgcttc aaatgtaccg actggattgc
      241 ttaatgaaat gattgcttat ctcaactcgg aagaacgcaa tcatcataat ttttcagaac
      301 ttttgctttt ttcttgcctg tctatttttg ccgcatgcaa aggtttcatt acactattaa
      361 ctaacggtgt gctatccgtt tccggaaaag tgagaaatat tgtcaacatg aagctggcgc
      421 acccatggaa gctgaaagat atttgtgact gcctgtacat cagtgaaagc ctgttgaaga
      481 aaaaactaaa gcaagagcaa acgacattct cacagattct attagatgcc agaatgcagc
      541 acgcaaaaaa tttgatacgc gtagaaggtt cagtcaataa aattgccgaa caatgtggtt
      601 atgccagtac atcttatttt atttatgcgt tccgcaaaca tttcggcaac agtccgaaga
      661 gagtttctaa ggagtaccgt tgtcaaagtc acacgggtat gaatacgggc aacacgatga
      721 gtgctttagc tatttgatta tttgctaacg agtagtcaac cacgcacgct acgtaagaat
      781 tatatggggc agccattccc tgccccgcgt tgtttttagg cgatatattt attgaaataa
      841 ataagtgaca tccatc
//
LOCUS       DAZKHF020000634          856 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2201S31241
            SAMN43267800-rid25106413.denovo.638, whole genome shotgun sequence.
ACCESSION   DAZKHF020000634 DAZKHF020000000
VERSION     DAZKHF020000634.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267800
            Sequence Read Archive: SRR30307263
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 856)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 856)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 856)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350581.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350581.2
            Genome Coverage       :: 151x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:21:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,652
            CDSs (total)                      :: 5,557
            Genes (coding)                    :: 5,320
            CDSs (with protein)               :: 5,320
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 237
            CDSs (without protein)            :: 237
            Pseudo Genes (ambiguous residues) :: 0 of 237
            Pseudo Genes (frameshifted)       :: 102 of 237
            Pseudo Genes (incomplete)         :: 130 of 237
            Pseudo Genes (internal stop)      :: 52 of 237
            Pseudo Genes (multiple problems)  :: 41 of 237
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..856
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267800-rid25106413.denovo.638"
                     /serotype="O 26:H11"
                     /isolate="2201S31241"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-01"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            complement(<1..444)
                     /gene="sctT"
                     /locus_tag="ACHOSC_RS26440"
                     /old_locus_tag="pgaptmp_005288"
     CDS             complement(<1..444)
                     /gene="sctT"
                     /locus_tag="ACHOSC_RS26440"
                     /old_locus_tag="pgaptmp_005288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000503299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0030254 - protein secretion by the type III
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system export apparatus
                     subunit SctT"
                     /protein_id="HGU4551008.1"
                     /translation="MGEAILYQLHSLLAATALGFCRLAPTFYLLPFFASGNIPTVVRH
                     PIIIVVSCALVQHYHYELLNLNEIDIALFAAREIIIGLFIACLLASPFWIFLAIGSFI
                     DNQRGATLSSTLDPATGVDTSELARLFNLFSAAVYLTKGGMNFILE"
     gene            complement(446..706)
                     /locus_tag="ACHOSC_RS26445"
                     /old_locus_tag="pgaptmp_005289"
     CDS             complement(446..706)
                     /locus_tag="ACHOSC_RS26445"
                     /old_locus_tag="pgaptmp_005289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EscS/YscS/HrcS family type III secretion system
                     export apparatus protein"
                     /protein_id="HGU4551009.1"
                     /translation="MDDIVFAGNRALYLILVMSAGPIAVATFVGLLVGLFQTVTQLQE
                     QTLPFGVKLLCVSICFFLMSGWYGEKLYSFGIEMLNLAFARG"
     gene            complement(716..>856)
                     /locus_tag="ACHOSC_RS26450"
                     /old_locus_tag="pgaptmp_005290"
     CDS             complement(716..>856)
                     /locus_tag="ACHOSC_RS26450"
                     /old_locus_tag="pgaptmp_005290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EscR/YscR/HrcR family type III secretion system
                     export apparatus protein"
                     /protein_id="HGU4551010.1"
                     /translation="LTLGMMMMSPVTISTPIKLILFVAMDGWTMLSKGLILQYFDLSI
                     NP"
BASE COUNT          295 a          193 c          144 g          224 t
ORIGIN      
        1 ttccagaata aagttcattc cacctttagt aagataaacc gcagcagaga aaaggttaaa
       61 taatcttgct aattccgagg tatcgacacc tgtagccgga tccaaagtac tacttaatgt
      121 tgcaccgcgt tggttatcga taaagctacc tatagcaagg aaaatccaaa acggggatgc
      181 taataagcat gcaataaaca gccctatgat aatttctctg gcagcgaaca aagcaatatc
      241 gatttcattt aagttcaata attcatagtg gtaatgctga actaatgcac atgataccac
      301 tattattatt gggtgacgaa ctactgttgg tatattccca ctggcgaaaa atggtaacaa
      361 atagaaagtt ggtgccaatc ggcaaaaacc gagcgctgta gcagccagca gactatgcaa
      421 ctgatagagt atcgcctccc ccatgctatc ctctcgcgaa tgctaaattc agcatttcga
      481 taccaaatga atataatttt tcaccatacc acccgctcat taaaaaaaag caaatactta
      541 cgcacaataa tttcacgcca aatggcaacg tctgttcttg caactgcgtt accgtttgaa
      601 aaagccctac caataaacca acaaatgtcg caactgcaat aggtcctgca gacataacta
      661 aaatcagata cagcgcccta ttccccgcaa aaacaatatc gtccatattt ttttctcagg
      721 gattaatgga taggtcaaaa tattgtaaaa tgagcccttt tgataacatt gtccaaccgt
      781 ccatcgcgac aaacaaaatt aattttatcg gtgtagatat ggttaccgga ctcatcatca
      841 tcatacctaa cgttaa
//
LOCUS       DAZKHF020000635          853 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2201S31241
            SAMN43267800-rid25106413.denovo.639, whole genome shotgun sequence.
ACCESSION   DAZKHF020000635 DAZKHF020000000
VERSION     DAZKHF020000635.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267800
            Sequence Read Archive: SRR30307263
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 853)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 853)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 853)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350581.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350581.2
            Genome Coverage       :: 151x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:21:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,652
            CDSs (total)                      :: 5,557
            Genes (coding)                    :: 5,320
            CDSs (with protein)               :: 5,320
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 237
            CDSs (without protein)            :: 237
            Pseudo Genes (ambiguous residues) :: 0 of 237
            Pseudo Genes (frameshifted)       :: 102 of 237
            Pseudo Genes (incomplete)         :: 130 of 237
            Pseudo Genes (internal stop)      :: 52 of 237
            Pseudo Genes (multiple problems)  :: 41 of 237
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..853
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267800-rid25106413.denovo.639"
                     /serotype="O 26:H11"
                     /isolate="2201S31241"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-01"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            <1..341
                     /locus_tag="ACHOSC_RS26455"
                     /old_locus_tag="pgaptmp_005291"
     CDS             <1..341
                     /locus_tag="ACHOSC_RS26455"
                     /old_locus_tag="pgaptmp_005291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HGU4551011.1"
                     /translation="TLYKHAKMVKAFDYILNHWNALNEFCRDGWVEIDNNIGENALRS
                     VAVGRKNYLFFGSDKGGESAAIIYSLLVTCKLNEVEPEDWLREVIVKLNDWPSNRVHE
                     LLPWNCSTVK"
     gene            complement(602..784)
                     /locus_tag="ACHOSC_RS26460"
                     /old_locus_tag="pgaptmp_005292"
     CDS             complement(602..784)
                     /locus_tag="ACHOSC_RS26460"
                     /old_locus_tag="pgaptmp_005292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000922600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4551012.1"
                     /translation="MLLAENPRQGMTTEARVVIRILLISDHVNRQDNTQIQTIMRIIY
                     ICFLQKVMIIDKTKYK"
     gene            723..>853
                     /locus_tag="ACHOSC_RS26465"
                     /old_locus_tag="pgaptmp_005293"
     CDS             723..>853
                     /locus_tag="ACHOSC_RS26465"
                     /old_locus_tag="pgaptmp_005293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HGU4551013.1"
                     /translation="MRITTLASVVIPCLGFSASSIAAAEDVMIVSASGYEKKLTNAAA
                     "
BASE COUNT          231 a          188 c          197 g          237 t
ORIGIN      
        1 aaactctgta taaacatgca aaaatggtaa aggcgttcga ctatatcctg aatcactgga
       61 atgcgctgaa cgagttctgt cgtgacggct gggtggaaat agacaacaac atcggtgaaa
      121 acgcgttacg atcggtggcg gttggaagaa aaaattatct cttcttcggc tcagacaagg
      181 ggggagaaag cgcggcgatc atctacagtc tgctggtcac ctgcaaactg aacgaagtgg
      241 agccggagga ctggttgcgc gaagtgatcg tgaagctcaa tgactggccg tcgaaccgtg
      301 tgcatgaact gctgccctgg aactgctcaa ctgtaaaata atccttacgc tacgtacttc
      361 tcggggcgct tacgaatttt tcacgtgcca cagaattttg aacgctttct cttacaatat
      421 ttcaatgttt ctatcagtat tcgccggaag aagtcatcga ccaaatcatc ccagtcgtct
      481 cgcaccactg accattcatg gttgacatgt ggtggaaatc cgcttctaca gtaaccattt
      541 tttattcgca aaaccgaaca cgccatacgg atagctgtta actggcatgc cgaggcagtc
      601 gttatttata tttagttttg tcaataatca ttactttttg caaaaagcaa atataaatta
      661 ttctcattat tgtttgtatt tgtgtattgt cttgccggtt aacatgatcg gagattagta
      721 atatgcgaat aaccactctg gcttccgtag tcattccctg tctcggattt tcagccagca
      781 gcatagctgc tgcagaggat gtgatgattg tctcggcatc cggctatgag aaaaagctga
      841 ctaacgcagc cgc
//
LOCUS       DAZKHF020000636          853 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2201S31241
            SAMN43267800-rid25106413.denovo.640, whole genome shotgun sequence.
ACCESSION   DAZKHF020000636 DAZKHF020000000
VERSION     DAZKHF020000636.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267800
            Sequence Read Archive: SRR30307263
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 853)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 853)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 853)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350581.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350581.2
            Genome Coverage       :: 151x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:21:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,652
            CDSs (total)                      :: 5,557
            Genes (coding)                    :: 5,320
            CDSs (with protein)               :: 5,320
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 237
            CDSs (without protein)            :: 237
            Pseudo Genes (ambiguous residues) :: 0 of 237
            Pseudo Genes (frameshifted)       :: 102 of 237
            Pseudo Genes (incomplete)         :: 130 of 237
            Pseudo Genes (internal stop)      :: 52 of 237
            Pseudo Genes (multiple problems)  :: 41 of 237
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..853
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267800-rid25106413.denovo.640"
                     /serotype="O 26:H11"
                     /isolate="2201S31241"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-01"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            <1..670
                     /locus_tag="ACHOSC_RS26470"
                     /old_locus_tag="pgaptmp_005294"
     CDS             <1..670
                     /locus_tag="ACHOSC_RS26470"
                     /old_locus_tag="pgaptmp_005294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016032 - viral process [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="phage tail tape measure protein"
                     /protein_id="HGU4551014.1"
                     /translation="QRADWQKGLQEGLSNWVDNASDYASQAAQLATEGISGMVNNITE
                     MLNGNKVEWRNWASSVLQEISKVLMNAAIVNGIKTAANGMSGAGGFIGSIGDWLGGAV
                     ANAKGGVYTSANLSAYSNSIVDTPTYFAFAKGAGLMGEAGPEAIMPLTRAADGSLGVR
                     AVGSMNGSAGLVYSPVYHIAIQNDGANGQIGAEAAGSLVQLIDQRVQAVMLSMRRDGG
                     MLSG"
     gene            663..>853
                     /locus_tag="ACHOSC_RS26475"
                     /old_locus_tag="pgaptmp_005295"
     CDS             663..>853
                     /locus_tag="ACHOSC_RS26475"
                     /old_locus_tag="pgaptmp_005295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HGU4551015.1"
                     /translation="MAEIKTLHLVPREGMQVSEKPSVVRVRFGDGYEQRRPTGLNARL
                     KTFQAVFRVTDEPTRRWLD"
BASE COUNT          193 a          194 c          304 g          162 t
ORIGIN      
        1 gcagcgtgcg gactggcaga aaggcttgca ggaggggctc agtaactggg tggacaatgc
       61 atccgattac gcttcacagg cagcacagct tgcgacggag ggtatctcag ggatggtgaa
      121 taacatcacg gagatgctga acggaaacaa agtggaatgg cgcaactggg cctcatccgt
      181 actgcaggaa atctcaaaag ttcttatgaa tgccgcgatt gtcaacggaa ttaagacggc
      241 ggcaaacggt atgtccggtg cgggaggatt tattggcagc attggtgact ggctgggcgg
      301 tgcggtggcc aatgcaaaag gcggcgtgta tacctcggca aacctgagtg cgtacagcaa
      361 cagcattgtg gacacgccca cgtactttgc gtttgcaaaa ggggccgggc tgatggggga
      421 agccggacct gaagccatta tgcccctgac ccgggcggcg gatggctcgc tgggcgtacg
      481 cgcggtgggc agtatgaacg gcagtgccgg tctggtgtat tccccggtct accacatcgc
      541 cattcagaat gacggggcta acggacagat aggggcggag gcggcaggca gtcttgtgca
      601 gctgattgac cagcgggtgc aggcggtgat gctgtccatg cgacgtgacg gaggaatgct
      661 gagtggctga gataaaaacg ctgcatctgg tcccgcgtga agggatgcag gtgagtgaga
      721 aaccgtcggt ggtgagggtt cggtttggtg acggttatga acagcgccga ccgacgggac
      781 ttaatgccag actgaagacg tttcaggcgg tgttccgggt gacggatgaa ccaacccggc
      841 gctggctgga tga
//
LOCUS       DAZKHF020000637          853 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2201S31241
            SAMN43267800-rid25106413.denovo.641, whole genome shotgun sequence.
ACCESSION   DAZKHF020000637 DAZKHF020000000
VERSION     DAZKHF020000637.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267800
            Sequence Read Archive: SRR30307263
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 853)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 853)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 853)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350581.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350581.2
            Genome Coverage       :: 151x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:21:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,652
            CDSs (total)                      :: 5,557
            Genes (coding)                    :: 5,320
            CDSs (with protein)               :: 5,320
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 237
            CDSs (without protein)            :: 237
            Pseudo Genes (ambiguous residues) :: 0 of 237
            Pseudo Genes (frameshifted)       :: 102 of 237
            Pseudo Genes (incomplete)         :: 130 of 237
            Pseudo Genes (internal stop)      :: 52 of 237
            Pseudo Genes (multiple problems)  :: 41 of 237
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..853
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267800-rid25106413.denovo.641"
                     /serotype="O 26:H11"
                     /isolate="2201S31241"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-01"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            complement(393..>853)
                     /locus_tag="ACHOSC_RS26480"
                     /old_locus_tag="pgaptmp_005296"
     CDS             complement(393..>853)
                     /locus_tag="ACHOSC_RS26480"
                     /old_locus_tag="pgaptmp_005296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="GTPase family protein"
                     /protein_id="HGU4551016.1"
                     /translation="ILHRGHQQVLFVVTQADKTEPCHEWDMAGIQPSPAQAQNIREKT
                     EAVFRLFRPVHPVVAVSARTGWELDTLVSALMTALPDHAASPLMTRLQDELRTESVRA
                     QAREQFTGAVDRIFDTAESVCVASVARTVLRAVRDTVVSVARAVWNWIFF"
BASE COUNT          206 a          226 c          228 g          193 t
ORIGIN      
        1 ggtgttcaga tgtcgtttca tcagcttttc cttacagagg gtgaataaaa aagccggtac
       61 ccacaaatgt ggataccggc aggtacaaaa cagcatgctc agataaagtg gtttttctcg
      121 gacataaatg cttcccctct ctcatccgcc agagaagtat cagtccgttg tgtgcaattc
      181 cgttctgcat gatgaagaga ttcagctgcc ttttcgcaga ccattaacca ccataaacga
      241 tcgatagcaa cgatcgatac ctgttttatc gatcgtttta ttctattaac gacaaggcgg
      301 tcacgcaaga gaaacaggag gagtatgagg ggtttgtgtc atgttttcag ttggttaacg
      361 taatgttatc aggcatttcc ctggcagact gttcagaaga aaatccagtt ccatacagcg
      421 cgggcaacag agaccaccgt gtcacggacg gcacgcagaa ccgtacgggc aacagaggca
      481 acacagacgc tctccgccgt gtcaaatatc cggtccaccg caccggtaaa ctgttcacgg
      541 gcctgagcgc ggacagattc cgtgcgcagc tcgtcctgca ggcgggtcat caggggactg
      601 gcggcatggt cgggaagtgc tgtcattagc gcactgacca gcgtatccag ttcccagccg
      661 gtgcgggccg atacggcaac aaccggatgt acgggccgga acagacggaa taccgcctcc
      721 gttttctccc gaatgttctg tgcctgtgcg ggagaaggct gaatgccggc catatcccat
      781 tcatggcagg gctccgtttt gtcggcctgc gtcaccacaa acagcacctg ctgatgtccc
      841 cggtgcagga tgt
//
LOCUS       DAZKHF020000638          843 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2201S31241
            SAMN43267800-rid25106413.denovo.642, whole genome shotgun sequence.
ACCESSION   DAZKHF020000638 DAZKHF020000000
VERSION     DAZKHF020000638.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267800
            Sequence Read Archive: SRR30307263
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 843)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 843)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 843)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350581.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350581.2
            Genome Coverage       :: 151x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:21:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,652
            CDSs (total)                      :: 5,557
            Genes (coding)                    :: 5,320
            CDSs (with protein)               :: 5,320
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 237
            CDSs (without protein)            :: 237
            Pseudo Genes (ambiguous residues) :: 0 of 237
            Pseudo Genes (frameshifted)       :: 102 of 237
            Pseudo Genes (incomplete)         :: 130 of 237
            Pseudo Genes (internal stop)      :: 52 of 237
            Pseudo Genes (multiple problems)  :: 41 of 237
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..843
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267800-rid25106413.denovo.642"
                     /serotype="O 26:H11"
                     /isolate="2201S31241"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-01"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            complement(<1..425)
                     /locus_tag="ACHOSC_RS26485"
                     /old_locus_tag="pgaptmp_005297"
     CDS             complement(<1..425)
                     /locus_tag="ACHOSC_RS26485"
                     /old_locus_tag="pgaptmp_005297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail length tape measure family protein"
                     /protein_id="HGU4551017.1"
                     /translation="MSQPVGDLIIDLSLDAVRFDEQMSRVRRHFSGLDTDARKTATAV
                     EQGLSRQALAAQKAGISVGQYKAAMRTLPAQFTDIATQLAGGQNPWLILLQQGGQVKD
                     SFGGMIPMLRGLAGAITLPMVGVTSLAVATGALAYAWYQG"
     gene            complement(406..>843)
                     /locus_tag="ACHOSC_RS26490"
                     /old_locus_tag="pgaptmp_005298"
     CDS             complement(406..>843)
                     /locus_tag="ACHOSC_RS26490"
                     /old_locus_tag="pgaptmp_005298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002213520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail assembly protein T"
                     /protein_id="HGU4551018.1"
                     /translation="FALRLAREMGRPDWRAMLAGMTSTEYADWRHFYRTHYFQDTQLD
                     MHFSGLMYAVLSLFFCDPDMHPSDFSLLVPRHEEEQVERLDEDKMLMQKAAGLAGGVR
                     FGGDGGGDILSSADVADVMVDDAALMMASAGIPGGVRYVPAGW"
BASE COUNT          192 a          279 c          212 g          160 t
ORIGIN      
        1 ccctggtacc aggcgtacgc cagcgcacct gtcgccaccg ccagcgaggt gaccccgacc
       61 atcggcaggg tgatcgcacc ggcaagcccc ctgagcatgg ggatcatccc gccgaaggag
      121 tccttcacct gaccgccctg ttgcagcagg atcagccagg gattctgacc accggcaagc
      181 tgcgtggcga tatccgtaaa ctgtgcgggc agggtgcgca tggccgcttt atactgtccg
      241 acggaaatcc cggctttttg cgcagccagc gcctgacggc tcaggccctg ttcaacagca
      301 gtggcggttt ttctggcgtc agtatccagt cctgaaaaat gacgccttac ccggctcatc
      361 tgctcatcga aacggaccgc atccagactc aggtcaataa taagatcacc aaccggctgg
      421 gacatatctc acacctcccg gaatccccgc tgaagccatc attaatgcgg catcatccac
      481 catgacatcc gccacatccg cagacgataa aatatcgccc cctccgtccc caccgaaccg
      541 gacgcctccg gcaagtcctg ccgctttctg catcagcatt ttgtcctcat ccagcctctc
      601 cacctgctct tcctcatgcc gggggacaag cagactgaaa tcagagggat gcatatccgg
      661 atcgcaaaaa aacaggctga gtacagcgta catcagcccg gaaaaatgca tatccagttg
      721 ggtatcctga aaataatgcg tgcggtaaaa atgtcgccag tcggcatatt cggtggatgt
      781 catcccggca agcatggcgc gccagtcagg cctccccatc tctcgcgcca gtctgagggc
      841 aaa
//
LOCUS       DAZKHF020000639          843 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2201S31241
            SAMN43267800-rid25106413.denovo.643, whole genome shotgun sequence.
ACCESSION   DAZKHF020000639 DAZKHF020000000
VERSION     DAZKHF020000639.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267800
            Sequence Read Archive: SRR30307263
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 843)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 843)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 843)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350581.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350581.2
            Genome Coverage       :: 151x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:21:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,652
            CDSs (total)                      :: 5,557
            Genes (coding)                    :: 5,320
            CDSs (with protein)               :: 5,320
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 237
            CDSs (without protein)            :: 237
            Pseudo Genes (ambiguous residues) :: 0 of 237
            Pseudo Genes (frameshifted)       :: 102 of 237
            Pseudo Genes (incomplete)         :: 130 of 237
            Pseudo Genes (internal stop)      :: 52 of 237
            Pseudo Genes (multiple problems)  :: 41 of 237
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..843
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267800-rid25106413.denovo.643"
                     /serotype="O 26:H11"
                     /isolate="2201S31241"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-01"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            <1..456
                     /locus_tag="ACHOSC_RS26495"
                     /old_locus_tag="pgaptmp_005299"
     CDS             <1..456
                     /locus_tag="ACHOSC_RS26495"
                     /old_locus_tag="pgaptmp_005299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage antirepressor KilAC domain-containing
                     protein"
                     /protein_id="HGU4551019.1"
                     /translation="LVDRWRELEGATAKIPQTFSEALRLAADLEDQKAELEKQLALAA
                     PKVEFADRVGEASGILIGNFAKVVGIGPNKLFAWMRDHKILIASGARRNVPMQEYMDR
                     GYFTVKETAVNTNHGIQISFTTKITGRGQQWLTRKLLDNGMLKVTGEAA"
     gene            456..>843
                     /locus_tag="ACHOSC_RS26500"
                     /old_locus_tag="pgaptmp_005300"
     CDS             456..>843
                     /locus_tag="ACHOSC_RS26500"
                     /old_locus_tag="pgaptmp_005300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination protein NinG"
                     /protein_id="HGU4551020.1"
                     /translation="MAKPARRKCKICKEWFHPAFSNQWWCSPEHGTKLALERRNKERE
                     KAEKTAEKKRRREEQKQKDKIKIRKLALKPRSYWIKQAQQAVNAFIRERDRDLPCISC
                     GTLTSAQWDAGHYRTTAAAPQLRFDER"
BASE COUNT          269 a          195 c          217 g          162 t
ORIGIN      
        1 cttgttgacc gctggcgaga actcgaaggg gcaaccgcga aaataccaca aaccttttct
       61 gaggcattgc gccttgcggc cgaccttgaa gaccagaagg ctgaactgga gaaacagctt
      121 gctctcgcag cacctaaagt tgagtttgcc gatcgagttg gcgaggccag cggaattttg
      181 attggaaact ttgcaaaggt tgttggtatt ggtccaaaca aactgtttgc gtggatgcgc
      241 gatcacaaaa tccttattgc ttcaggtgcc cggcgcaatg tgccaatgca ggaatatatg
      301 gatcgcggct atttcacagt gaaagaaaca gcggtcaata caaatcacgg aatacagata
      361 tcgttcacca caaaaatcac cgggcgtggt caacagtggc tgacaagaaa gctgctagat
      421 aacggaatgc ttaaagtaac aggggaggct gcttaatggc taaaccagcg cgaaggaaat
      481 gcaaaatctg taaggaatgg tttcacccgg cattctcaaa tcagtggtgg tgcagcccgg
      541 aacacggaac taaattagcg ctcgaacgac gaaataaaga acgcgaaaag gcggaaaaaa
      601 cagcagagaa gaaacgacga cgagaggagc agaaacagaa agataaaatt aagattcgaa
      661 aactcgcctt aaagccccgc agttactgga ttaaacaagc ccaacaagcc gtaaacgcct
      721 tcatcagaga aagagaccgc gacttaccat gtatctcgtg cggaacgctc acgtctgctc
      781 agtgggatgc cggacattac cggacaactg ctgcggcacc tcaactccga tttgatgaac
      841 gca
//
LOCUS       DAZKHF020000640          842 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2201S31241
            SAMN43267800-rid25106413.denovo.644, whole genome shotgun sequence.
ACCESSION   DAZKHF020000640 DAZKHF020000000
VERSION     DAZKHF020000640.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267800
            Sequence Read Archive: SRR30307263
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 842)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 842)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 842)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350581.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350581.2
            Genome Coverage       :: 151x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:21:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,652
            CDSs (total)                      :: 5,557
            Genes (coding)                    :: 5,320
            CDSs (with protein)               :: 5,320
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 237
            CDSs (without protein)            :: 237
            Pseudo Genes (ambiguous residues) :: 0 of 237
            Pseudo Genes (frameshifted)       :: 102 of 237
            Pseudo Genes (incomplete)         :: 130 of 237
            Pseudo Genes (internal stop)      :: 52 of 237
            Pseudo Genes (multiple problems)  :: 41 of 237
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..842
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267800-rid25106413.denovo.644"
                     /serotype="O 26:H11"
                     /isolate="2201S31241"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-01"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            6..284
                     /locus_tag="ACHOSC_RS26505"
                     /old_locus_tag="pgaptmp_005301"
     CDS             6..284
                     /locus_tag="ACHOSC_RS26505"
                     /old_locus_tag="pgaptmp_005301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4551021.1"
                     /translation="MAHDTKLYNSDDSAVFASRRGRCFHAFKSDWYQHPPCTEEQAEW
                     LIQCYRRRGCEVKKALSLDYRHWIISVRLPYSERPPRPSRTFQQRIWR"
     gene            286..>842
                     /locus_tag="ACHOSC_RS26510"
                     /old_locus_tag="pgaptmp_005302"
     CDS             286..>842
                     /locus_tag="ACHOSC_RS26510"
                     /old_locus_tag="pgaptmp_005302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF968 domain-containing protein"
                     /protein_id="HGU4551022.1"
                     /translation="MRVLLRPVLVPELGLVIVKPGRESMRVFHGGRVLVESEPKSMRN
                     LPSGVVPAVRQPLAEDKSLLPFFSNERVIRAAGGAGALSDWLLRHVKSCQWPHGDYHH
                     SETVIHRYGTGAMVLCWHCDNQLRDQTSESLEQLAQQNLSAWMIDVIRHAMNGIQERE
                     LSLAELSWWAVCNQVVDALPEAVSRR"
BASE COUNT          187 a          225 c          228 g          202 t
ORIGIN      
        1 aaccgatggc gcacgatacc aaattataca attctgatga ttctgccgtc tttgccagca
       61 ggcgcggacg gtgttttcac gcattcaaat ctgactggta ccagcatccc ccatgcactg
      121 aagaacaggc cgaatggctc attcagtgtt accgcaggcg cggatgcgag gttaaaaaag
      181 cccttagcct cgactaccgt cactggataa tctccgtcag gctcccttac tccgaacggc
      241 caccgcgtcc gtcccgcaca ttccagcaac ggatctggag gtaatgtgcg ggtattactt
      301 cgacctgttc tggtaccgga actcggtctg gttatcgtta agccaggccg tgaatccatg
      361 cgggtatttc atggcggcag ggtgctggtg gagtctgaac cgaaaagcat gcgtaatctg
      421 ccgtccgggg tcgttcctgc cgttcgccag ccgctggcgg aagataaatc attactgcca
      481 tttttcagca atgagcgtgt gattcgtgct gctggtggcg ctggtgcact gtctgactgg
      541 ttattacgtc acgttaaatc ctgccagtgg cctcatggtg attatcatca cagcgaaact
      601 gtcatacatc gttacggtac cggcgcgatg gtgttgtgct ggcactgcga caaccagctg
      661 cgcgaccaga catccgaatc acttgagcaa cttgctcaac aaaacctgtc agcatggatg
      721 attgacgtca tccgccacgc aatgaatggc atacaggaac gggaattatc gctggctgaa
      781 ttatcctggt gggcagtctg caatcaggtg gtggacgcat tacctgaggc agtatcgcgt
      841 cg
//
LOCUS       DAZKHF020000641          841 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2201S31241
            SAMN43267800-rid25106413.denovo.645, whole genome shotgun sequence.
ACCESSION   DAZKHF020000641 DAZKHF020000000
VERSION     DAZKHF020000641.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267800
            Sequence Read Archive: SRR30307263
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 841)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 841)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 841)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350581.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350581.2
            Genome Coverage       :: 151x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:21:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,652
            CDSs (total)                      :: 5,557
            Genes (coding)                    :: 5,320
            CDSs (with protein)               :: 5,320
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 237
            CDSs (without protein)            :: 237
            Pseudo Genes (ambiguous residues) :: 0 of 237
            Pseudo Genes (frameshifted)       :: 102 of 237
            Pseudo Genes (incomplete)         :: 130 of 237
            Pseudo Genes (internal stop)      :: 52 of 237
            Pseudo Genes (multiple problems)  :: 41 of 237
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..841
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267800-rid25106413.denovo.645"
                     /serotype="O 26:H11"
                     /isolate="2201S31241"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-01"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            <1..>841
                     /locus_tag="ACHOSC_RS26515"
                     /old_locus_tag="pgaptmp_005303"
     CDS             <1..>841
                     /locus_tag="ACHOSC_RS26515"
                     /old_locus_tag="pgaptmp_005303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000281525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004559 - alpha-mannosidase activity
                     [Evidence IEA];
                     GO_process: GO:0006013 - mannose metabolic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="glycoside hydrolase family 38 C-terminal
                     domain-containing protein"
                     /protein_id="HGU4551023.1"
                     /translation="KRYGKEYITENMHSEIRAYHEDAGFFAAWDFASNYRDGESYVLL
                     AEKMTTVISGPKTTMTLIYHYNSSYLRFAFTLTQDSPRVDVQTFIDWHEPNVSLKVKF
                     PVSVQTSLAQCQIQFGVIDRPTHSDDSFAFAKDEIPAHHWVDLSDQETGIALIAKNKY
                     GYRVKDQTLELTLLRSQHKPGEVVKTDNYDNFLINNFADIGKQKFTFSLFPHHGDYRV
                     GGVVNQAYIMNHPLQIVPVKPHPGELPPSLSFFAIDTNSVIIETIKLAEDGDGIIVRL
                     YES"
BASE COUNT          261 a          177 c          158 g          245 t
ORIGIN      
        1 ataaaagata tggtaaagaa tacattactg aaaatatgca cagtgagatc cgtgcatacc
       61 acgaagatgc aggttttttc gcggcctggg attttgcttc aaattatcgt gatggggaat
      121 catatgtttt actcgctgaa aaaatgacaa ctgtgatcag cgggcctaaa actacgatga
      181 cgctgattta tcactacaat tcctcttatc ttcgcttcgc atttaccctg acacaggaca
      241 gtccacgcgt tgatgtccaa acttttattg attggcacga acctaatgtt tctctcaaag
      301 taaaattccc ggtatcagta caaacatctc ttgcacaatg tcaaattcag ttcggtgtaa
      361 ttgacagacc aacgcatagc gatgattcgt tcgcttttgc caaagatgaa ataccagcac
      421 atcactgggt cgacctttct gatcaagaaa ctggcattgc tttaatagca aaaaacaaat
      481 atggctatcg tgtcaaagac cagacgctag agttaacttt gttgcgttcg cagcacaaac
      541 ctggggaagt tgttaagaca gataattatg ataatttcct gattaataat tttgcagata
      601 ttggtaaaca aaaattcaca ttctccttat ttcctcatca tggagattac cgtgtgggtg
      661 gtgtcgttaa ccaggcctat ataatgaatc atcccttaca gatcgtccct gtcaaaccac
      721 acccgggtga actaccccct tcactatcat tttttgctat cgatactaac agcgttatca
      781 tcgaaacaat aaaattagct gaagacggcg acggtataat cgtaagatta tatgagagtt
      841 a
//
LOCUS       DAZKHF020000642          838 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2201S31241
            SAMN43267800-rid25106413.denovo.646, whole genome shotgun sequence.
ACCESSION   DAZKHF020000642 DAZKHF020000000
VERSION     DAZKHF020000642.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267800
            Sequence Read Archive: SRR30307263
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 838)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 838)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 838)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350581.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350581.2
            Genome Coverage       :: 151x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:21:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,652
            CDSs (total)                      :: 5,557
            Genes (coding)                    :: 5,320
            CDSs (with protein)               :: 5,320
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 237
            CDSs (without protein)            :: 237
            Pseudo Genes (ambiguous residues) :: 0 of 237
            Pseudo Genes (frameshifted)       :: 102 of 237
            Pseudo Genes (incomplete)         :: 130 of 237
            Pseudo Genes (internal stop)      :: 52 of 237
            Pseudo Genes (multiple problems)  :: 41 of 237
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..838
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267800-rid25106413.denovo.646"
                     /serotype="O 26:H11"
                     /isolate="2201S31241"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-01"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            complement(36..122)
                     /locus_tag="ACHOSC_RS26520"
                     /old_locus_tag="pgaptmp_005304"
     CDS             complement(36..122)
                     /locus_tag="ACHOSC_RS26520"
                     /old_locus_tag="pgaptmp_005304"
                     /inference="COORDINATES: protein motif:HMM:NF024464.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3927 family protein"
                     /protein_id="HGU4551024.1"
                     /translation="MSVLADCVLVAGVVVVAFPLLKKKTPDC"
     gene            complement(667..743)
                     /locus_tag="ACHOSC_RS26525"
                     /old_locus_tag="pgaptmp_005305"
     tRNA            complement(667..743)
                     /locus_tag="ACHOSC_RS26525"
                     /old_locus_tag="pgaptmp_005305"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(707..709),aa:Arg,seq:tct)
BASE COUNT          268 a          212 c          152 g          206 t
ORIGIN      
        1 gtatttaaaa atttctcccg ctaactgata cccggctaac agtctggcgt tttctttttc
       61 agcaacggga aagcaacaac caccacaccc gccaccagta cacagtcagc cagcactgac
      121 attatccggc tgctgcaatg ccattcacaa aaacagtaag caatcacttt ttaccgtaac
      181 aggtgataat ccagatatgt atctacccca gatgagtaat ccgaagttca tccataccac
      241 aggtcctggc tattctgttg tactcctgaa caagagcaaa taattctgaa ttagcaacca
      301 tgaactcatc gcaaaccctc tgtatagcat cactattcag aagaataacg tctcttcccg
      361 aaagacgatc aggagtacag aacaaaactg tcaaacggct gaaggccttt gctcgtgctg
      421 cattgactat atcaatacgc tgcctaagga tgaaacaccc cgacgcctca tcaatattca
      481 ctctacccac accatatgaa tgataaatat ttaatactga aaaaaccatt agaccgtata
      541 acaaacactc aatcaatact taacagaact tttatttttg acaaacatat aatattttca
      601 acaatatcct gagccaggta tattccagta taaggctctg ccggaagaaa tctggaagaa
      661 tgaatatggc gcgttgtact ggattcgaac cagtgaccga ttgcttagaa ggcaattgct
      721 ctgtccggct gagctaacaa cgcataatgc agataatgga ttgccatcgg ggacccgggc
      781 cccacacagc cagtttcgaa agctgacgct ctctgccgat gagctaatgg cggtatgt
//
LOCUS       DAZKHF020000643          837 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2201S31241
            SAMN43267800-rid25106413.denovo.647, whole genome shotgun sequence.
ACCESSION   DAZKHF020000643 DAZKHF020000000
VERSION     DAZKHF020000643.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267800
            Sequence Read Archive: SRR30307263
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 837)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 837)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 837)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350581.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350581.2
            Genome Coverage       :: 151x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:21:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,652
            CDSs (total)                      :: 5,557
            Genes (coding)                    :: 5,320
            CDSs (with protein)               :: 5,320
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 237
            CDSs (without protein)            :: 237
            Pseudo Genes (ambiguous residues) :: 0 of 237
            Pseudo Genes (frameshifted)       :: 102 of 237
            Pseudo Genes (incomplete)         :: 130 of 237
            Pseudo Genes (internal stop)      :: 52 of 237
            Pseudo Genes (multiple problems)  :: 41 of 237
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..837
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267800-rid25106413.denovo.647"
                     /serotype="O 26:H11"
                     /isolate="2201S31241"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-01"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            69..479
                     /locus_tag="ACHOSC_RS26530"
                     /old_locus_tag="pgaptmp_005306"
     CDS             69..479
                     /locus_tag="ACHOSC_RS26530"
                     /old_locus_tag="pgaptmp_005306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001129164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4551025.1"
                     /translation="MAKRKNNVVKKIGDSASLLSKPKSILRRKDFKELVTLTKQNSAP
                     GEWKTEIIEHSPSVPCGDEFNALQEILSSTPGVFWKPRKRKEYIVDSSDLRKYQILGF
                     EDYNHYVGYLATNGLNNLVPDFQILDNADHYGDF"
     gene            481..816
                     /locus_tag="ACHOSC_RS26535"
                     /old_locus_tag="pgaptmp_005307"
     CDS             481..816
                     /locus_tag="ACHOSC_RS26535"
                     /old_locus_tag="pgaptmp_005307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001039288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4551026.1"
                     /translation="MNKNTYDTIYSLINYYEDDYLLPLNRAELEAYKENTPASLNEAF
                     KHWDLAVNAFEHLSKRVEMLCKRENAYLTADQIWELSNWIEDIESDVRYVGDGLVELA
                     QRLGTITEE"
BASE COUNT          294 a          165 c          159 g          219 t
ORIGIN      
        1 attgttcttt ctcacgttag aaacatactt cggggtgttt tcctttaaaa gaaaaggaaa
       61 agtaaataat ggcaaaaaga aaaaacaacg ttgttaagaa aatcggggat tcagcttctc
      121 ttctaagtaa acctaaatct attctaagac gcaaagactt taaagagcta gttacgctca
      181 ccaagcaaaa tagcgcccct ggcgaatgga aaacagagat catagaacat tctccctctg
      241 tgccttgcgg agatgaattt aacgccttac aagaaatctt atcttcaaca cctggcgtat
      301 tctggaaacc tagaaaaaga aaagagtata tcgttgatag ctctgattta cgcaaatacc
      361 agattttagg atttgaagac tataaccact acgttggtta tctcgccaca aatggcctaa
      421 ataacttagt tccagacttc cagattctag ataatgctga tcactatgga gacttttaat
      481 atgaacaaaa acacgtatga cacaatttac tcactaatta attattatga ggatgattac
      541 cttctgcctt taaaccgcgc tgaacttgaa gcatacaaag aaaacacacc agcgtcacta
      601 aatgaggcgt ttaaacactg ggatttagct gtgaatgcct ttgagcacct ctctaagcgt
      661 gtagagatgc tctgtaagcg cgaaaacgca tacctgacag ccgatcagat atgggagcta
      721 tcaaactgga tagaagatat cgaatctgat gttcgctatg tgggagatgg acttgtcgaa
      781 ctcgctcaac gcttaggcac tatcacagaa gaataaaaaa agcaacaaaa aagttgt
//
LOCUS       DAZKHF020000644          834 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2201S31241
            SAMN43267800-rid25106413.denovo.648, whole genome shotgun sequence.
ACCESSION   DAZKHF020000644 DAZKHF020000000
VERSION     DAZKHF020000644.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267800
            Sequence Read Archive: SRR30307263
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 834)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 834)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 834)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350581.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350581.2
            Genome Coverage       :: 151x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:21:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,652
            CDSs (total)                      :: 5,557
            Genes (coding)                    :: 5,320
            CDSs (with protein)               :: 5,320
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 237
            CDSs (without protein)            :: 237
            Pseudo Genes (ambiguous residues) :: 0 of 237
            Pseudo Genes (frameshifted)       :: 102 of 237
            Pseudo Genes (incomplete)         :: 130 of 237
            Pseudo Genes (internal stop)      :: 52 of 237
            Pseudo Genes (multiple problems)  :: 41 of 237
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..834
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267800-rid25106413.denovo.648"
                     /serotype="O 26:H11"
                     /isolate="2201S31241"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-01"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            complement(<1..624)
                     /locus_tag="ACHOSC_RS26540"
                     /old_locus_tag="pgaptmp_005308"
     CDS             complement(<1..624)
                     /locus_tag="ACHOSC_RS26540"
                     /old_locus_tag="pgaptmp_005308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF932 domain-containing protein"
                     /protein_id="HGU4551027.1"
                     /translation="MRLASRFGYANQIRRDRPLTHEELMHYVPGIFGEDKHTSRSQNY
                     TYIPTITVLESLQREGFQPFFACQTRVRDPGRRGYTKHMLRLRRAGEINGEHVPEIIL
                     LNSHDGTSSYQMLPGYFRFVCQNGCVCGQSLGEVRVPHRGNVVDRVIEGAYEVVGVFD
                     RIEEKRDAMQSLILPPPARQALAQAALTYRYGDEHQPVTTADILTPRR"
     gene            complement(724..789)
                     /locus_tag="ACHOSC_RS26545"
                     /old_locus_tag="pgaptmp_005309"
                     /pseudo
     CDS             complement(724..789)
                     /locus_tag="ACHOSC_RS26545"
                     /old_locus_tag="pgaptmp_005309"
                     /inference="COORDINATES: protein motif:HMM:NF017787.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            760..>834
                     /locus_tag="ACHOSC_RS26550"
                     /old_locus_tag="pgaptmp_005310"
     CDS             760..>834
                     /locus_tag="ACHOSC_RS26550"
                     /old_locus_tag="pgaptmp_005310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4551028.1"
                     /translation="MKHLSGIGFRIPAAPASLTFRVADN"
BASE COUNT          173 a          234 c          236 g          191 t
ORIGIN      
        1 gcgtcgtggc gtcagaatgt cggcggtggt gacgggctga tgttcgtcac cataacggta
       61 agtcagtgcc gcctgtgcca gcgcctggcg tgccggtggc ggcagaatca gcgactgcat
      121 ggcatcacgc ttttcctcaa tccggtcaaa cacgcccacc acctcgtaag ccccttcaat
      181 gactctgtcc accacatttc cccggtgcgg aacacgcact tcccccagag actgaccaca
      241 gacgcatccg ttctggcaga cgaacctgaa gtaacccggc agcatctggt agctggaggt
      301 accgtcatga gagttgagca gaataatttc agggacatgt tctccgttta tctctccggc
      361 ccgccgcaga cgcagcatgt gttttgtgta tccccggcgg cccgggtcgc gcacacgggt
      421 ctggcaggcg aagaatggct gaaagccttc ccgctgcagg ctttccagta cggtgatggt
      481 ggggatgtac gtatagttct ggcttcggga cgtgtgttta tcttccccga aaatccccgg
      541 cacatagtgc atcagttctt cgtgtgtcag cggacggtca cggcgtatct ggttcgcata
      601 accaaaacga ctggctagtc gcataatttg ctccttatcg gtggttaaga tttactggtg
      661 taataaatga aaaagccacg tctcccggag aagacgcggc ctgatgggtg aaatgaatga
      721 cgtttattgt ctgagaagac cgtcactggc gagccgcgta ttaagcacct ttccggcatc
      781 gggttcagaa ttccggcagc gccagcgtcg ctgaccttcc gcgttgcgga taac
//
LOCUS       DAZKHF020000645          834 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2201S31241
            SAMN43267800-rid25106413.denovo.649, whole genome shotgun sequence.
ACCESSION   DAZKHF020000645 DAZKHF020000000
VERSION     DAZKHF020000645.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267800
            Sequence Read Archive: SRR30307263
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 834)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 834)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 834)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350581.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350581.2
            Genome Coverage       :: 151x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:21:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,652
            CDSs (total)                      :: 5,557
            Genes (coding)                    :: 5,320
            CDSs (with protein)               :: 5,320
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 237
            CDSs (without protein)            :: 237
            Pseudo Genes (ambiguous residues) :: 0 of 237
            Pseudo Genes (frameshifted)       :: 102 of 237
            Pseudo Genes (incomplete)         :: 130 of 237
            Pseudo Genes (internal stop)      :: 52 of 237
            Pseudo Genes (multiple problems)  :: 41 of 237
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..834
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267800-rid25106413.denovo.649"
                     /serotype="O 26:H11"
                     /isolate="2201S31241"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-01"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            <1..746
                     /locus_tag="ACHOSC_RS26555"
                     /old_locus_tag="pgaptmp_005311"
     CDS             <1..746
                     /locus_tag="ACHOSC_RS26555"
                     /old_locus_tag="pgaptmp_005311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012904434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="IS110-like element IS621 family transposase"
                     /protein_id="HGU4551029.1"
                     /translation="VSVINPALGKAFAQSEGLRNKTDTVDARMLAEFCRQKRPAAWEA
                     PHPLERALRALVVRHQALTDMHTQELNRTETAREVQRPSIDAHLLWLEAELKRLEKQI
                     KDLTDDDPDMKHRRKLLESIPGIGEKTSAVLLAYIGLKDRFAHARQFAAFAGLTPRRY
                     ESGSSVRGASRMSKAGHVSLRRALYMPAMVATSKTEWGRAFRDRLAANGKKGKVILGA
                     MMRKLAQVAYGVLKSGVPFDASRHNPVAA"
BASE COUNT          204 a          205 c          267 g          158 t
ORIGIN      
        1 tagtgtcagt cattaatcct gcgctgggta aagctttcgc tcagagtgaa ggactgcgta
       61 acaagactga taccgtggat gcgcgcatgc tggcagagtt ctgtcgtcag aagcgccctg
      121 cagcctggga agcgcctcac ccgcttgaac gcgcgttgcg tgccctggta gtccgccacc
      181 aggcgctgac agatatgcac acgcaggaac tgaatcgcac tgaaacggcg cgggaagtcc
      241 agagaccgag cattgatgct caccttctgt ggcttgaagc agagctgaag cgtcttgaga
      301 agcagataaa agacctgaca gacgatgatc cggatatgaa acaccgcagg aaactgctgg
      361 aaagcatccc gggtatcgga gagaaaacat ctgcggtatt gctggcttat atcggtctga
      421 aggaccgctt cgcccatgcc agacagttcg ccgcttttgc gggtctgaca ccacggcgtt
      481 atgaatcagg tagcagtgtg agaggggcga gccggatgag taaggccgga catgtgtcgc
      541 ttcgcagggc gttgtatatg cccgcaatgg tagccaccag taagactgag tggggacggg
      601 cgttccgcga ccgtctggcg gctaatggca agaaaggaaa ggtgattctc ggcgcgatga
      661 tgcgcaagct ggcacaggtg gcgtatggag tgctgaagtc aggcgtgccg ttcgatgcgt
      721 cacggcataa tccggtagcg gcgtaaaaat cgcggaaggg atgaaaaaaa cagcgcctga
      781 cggcgctgtg tctggcatgc ctgcaatccg ggaaaccgga ccaggaaaaa actt
//
LOCUS       DAZKHF020000646          830 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2201S31241
            SAMN43267800-rid25106413.denovo.650, whole genome shotgun sequence.
ACCESSION   DAZKHF020000646 DAZKHF020000000
VERSION     DAZKHF020000646.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267800
            Sequence Read Archive: SRR30307263
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 830)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 830)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 830)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350581.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350581.2
            Genome Coverage       :: 151x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:21:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,652
            CDSs (total)                      :: 5,557
            Genes (coding)                    :: 5,320
            CDSs (with protein)               :: 5,320
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 237
            CDSs (without protein)            :: 237
            Pseudo Genes (ambiguous residues) :: 0 of 237
            Pseudo Genes (frameshifted)       :: 102 of 237
            Pseudo Genes (incomplete)         :: 130 of 237
            Pseudo Genes (internal stop)      :: 52 of 237
            Pseudo Genes (multiple problems)  :: 41 of 237
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..830
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267800-rid25106413.denovo.650"
                     /serotype="O 26:H11"
                     /isolate="2201S31241"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-01"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            <1..284
                     /locus_tag="ACHOSC_RS26560"
                     /old_locus_tag="pgaptmp_005312"
     CDS             <1..284
                     /locus_tag="ACHOSC_RS26560"
                     /old_locus_tag="pgaptmp_005312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF4049 domain-containing protein"
                     /protein_id="HGU4551030.1"
                     /translation="RSNIMKPTAVYADYINNTTDFRPKPERIFICGETLKCEDPSIYI
                     RQKYGHHGVGVDQNQQFDNGVMGLNSTKKVLDKNNKIIYSSGLSCFQSH"
     gene            546..>830
                     /locus_tag="ACHOSC_RS26565"
                     /old_locus_tag="pgaptmp_005313"
     CDS             546..>830
                     /locus_tag="ACHOSC_RS26565"
                     /old_locus_tag="pgaptmp_005313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4551031.1"
                     /translation="MKIGTVAGTNDSTTTIATNDMVQEHVTNFTKELFGYIANGIGDD
                     ISSIARTMLGEVVEKIDDWQIERFQQSIRDDKISFTIQTNHSEKYSMLSGM"
BASE COUNT          287 a          132 c          160 g          251 t
ORIGIN      
        1 tccgaagcaa tattatgaaa cccacagctg tatatgctga ttatattaat aacactactg
       61 atttcagacc aaaacctgaa cgtatcttca tatgtggaga gacactcaaa tgtgaagatc
      121 ccagcatata cattagacaa aaatatggcc atcatggggt cggggtagat cagaatcaac
      181 aatttgataa tggtgttatg gggctaaatt ccacgaagaa agtacttgat aaaaataaca
      241 aaataattta ttcctcaggg cttagttgtt ttcaatctca ttaaaactcc atgaatacgg
      301 tggtatgcct ttttaatcct gccagtattt cataggtttt cttaatcgat tgtagaggta
      361 tgtactgatg acgcctctac aatctttagg gactttctct gctatgagat aagaaatttc
      421 atattatgct ttaaggctga atgatttgtg caaaatataa ttatttttcc atcgaacatg
      481 aggtgtatgt cataagatta aatgttaact atgcattcct cgcggtgaat tatatgaggt
      541 aaaagatgaa aattggaact gtggcaggca ctaacgacag tacaacgaca atcgcaacaa
      601 atgatatggt gcaagagcat gttactaact tcactaaaga actgtttggg tatattgcaa
      661 atggtatagg tgatgatata tcaagtattg ccaggacaat gctcggtgaa gttgtggaaa
      721 aaattgatga ttggcaaata gaacgtttcc aacaatctat tcgagatgac aaaatttcat
      781 ttaccataca aacgaatcat tcagaaaaat atagtatgct atcgggcatg
//
LOCUS       DAZKHF020000647          827 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2201S31241
            SAMN43267800-rid25106413.denovo.651, whole genome shotgun sequence.
ACCESSION   DAZKHF020000647 DAZKHF020000000
VERSION     DAZKHF020000647.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267800
            Sequence Read Archive: SRR30307263
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 827)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 827)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 827)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350581.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350581.2
            Genome Coverage       :: 151x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:21:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,652
            CDSs (total)                      :: 5,557
            Genes (coding)                    :: 5,320
            CDSs (with protein)               :: 5,320
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 237
            CDSs (without protein)            :: 237
            Pseudo Genes (ambiguous residues) :: 0 of 237
            Pseudo Genes (frameshifted)       :: 102 of 237
            Pseudo Genes (incomplete)         :: 130 of 237
            Pseudo Genes (internal stop)      :: 52 of 237
            Pseudo Genes (multiple problems)  :: 41 of 237
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..827
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267800-rid25106413.denovo.651"
                     /serotype="O 26:H11"
                     /isolate="2201S31241"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-01"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            complement(26..546)
                     /locus_tag="ACHOSC_RS26570"
                     /old_locus_tag="pgaptmp_005314"
                     /pseudo
     CDS             complement(26..546)
                     /locus_tag="ACHOSC_RS26570"
                     /old_locus_tag="pgaptmp_005314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000332260.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            590..670
                     /locus_tag="ACHOSC_RS26575"
                     /old_locus_tag="pgaptmp_005315"
                     /pseudo
     CDS             590..670
                     /locus_tag="ACHOSC_RS26575"
                     /old_locus_tag="pgaptmp_005315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309678.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tail fiber assembly protein"
BASE COUNT          226 a          161 c          186 g          254 t
ORIGIN      
        1 ggttcagcat agcctaggct gaacttttga ggatatcaac gaacggaatt tttgaacatt
       61 tatgagtaga tcgttgttga agggcttcaa tgagcatgtc aagttcatca actgctggtc
      121 tacgattacg acggtttgat ttaccaatca aagcaagttt aagtagatat ggacgagcac
      181 ttttcgccgg gtttgatgtg taattaattc cgtatatagg tttggctgca tccagaacac
      241 tgccaagata actaacatcg tgactgactg ttgctggacc tgcaccagcg ttgtttctca
      301 gcctgcaatg ttcaattacg tcattttctg tcagttcaga tagtttgatc gcggagatgt
      361 cactatccat aagcagttct agcacatatc ttttagtacg gtctgcttta cctccggcat
      421 ttggtcattt aaatatttgt gtagtaagtc acggactgta agtccgtcaa cagcatttga
      481 tgatgaaatg ccatatagat ctaataccat cactttctgt gtgccccatg ttttagcatg
      541 agcatgaacg cggcggctat ctcacgggtg atcatccctt tggcatactg aaaagtatcg
      601 ggtgttactg aaccgtgttg atacatcaac tgcactggat attgagtggc cgataatacc
      661 gacgttataa cctataaatg ccaatcaggc tgaatgttga aaggatagaa tgaacagccc
      721 gacacacaaa aaacggagtc cggctccggt ttttgtgttg ccatataagg cagatgtttg
      781 ttacagctat ttaagtctgg agttcaaatt aaaataggga gttttgt
//
LOCUS       DAZKHF020000648          826 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2201S31241
            SAMN43267800-rid25106413.denovo.652, whole genome shotgun sequence.
ACCESSION   DAZKHF020000648 DAZKHF020000000
VERSION     DAZKHF020000648.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267800
            Sequence Read Archive: SRR30307263
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 826)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 826)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 826)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350581.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350581.2
            Genome Coverage       :: 151x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:21:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,652
            CDSs (total)                      :: 5,557
            Genes (coding)                    :: 5,320
            CDSs (with protein)               :: 5,320
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 237
            CDSs (without protein)            :: 237
            Pseudo Genes (ambiguous residues) :: 0 of 237
            Pseudo Genes (frameshifted)       :: 102 of 237
            Pseudo Genes (incomplete)         :: 130 of 237
            Pseudo Genes (internal stop)      :: 52 of 237
            Pseudo Genes (multiple problems)  :: 41 of 237
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..826
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267800-rid25106413.denovo.652"
                     /serotype="O 26:H11"
                     /isolate="2201S31241"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-01"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            complement(<1..198)
                     /locus_tag="ACHOSC_RS26580"
                     /old_locus_tag="pgaptmp_005316"
     CDS             complement(<1..198)
                     /locus_tag="ACHOSC_RS26580"
                     /old_locus_tag="pgaptmp_005316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308874.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M85 family metallopeptidase"
                     /protein_id="HGU4551032.1"
                     /translation="MYKTILSINMKIPVLQPGFNFFAPAGYSAAVAPNRAENAYADYV
                     LDIGKRIPLSAADLSNVYESVI"
     gene            complement(601..>826)
                     /locus_tag="ACHOSC_RS26585"
                     /old_locus_tag="pgaptmp_005317"
     CDS             complement(601..>826)
                     /locus_tag="ACHOSC_RS26585"
                     /old_locus_tag="pgaptmp_005317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310967.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="phage tail fiber C-terminal domain-containing
                     protein"
                     /protein_id="HGU4551033.1"
                     /translation="KHDGREHVQSPTAQLRLGPADILESDENGIIPEQDRVITQVVIL
                     DADKKQIQCVVRPLQILRADGTWENIGGMK"
BASE COUNT          226 a          206 c          157 g          237 t
ORIGIN      
        1 aattacactt tcgtatacgt tgcttaaatc tgctgcggaa agcggtattc gcttgcctat
       61 atccaaaacg taatccgcat aggcattttc agcacgatta ggagcaacgg cagcagagta
      121 tccagcaggg gcaaaaaagt tgaagccagg ttggagaacg ggaattttca tattgatact
      181 taatatggtt ttatatattt cagtgccacc aattcactag cttacaacca gaaactaaaa
      241 aaacgtcagg gcgttgataa taatgaatac caactttctg cccatttata gtaatatctt
      301 tcgttatcag gttttgtctg gcagcttcac gtaatatttc aatactctgc aaagcaacat
      361 ttgatgaaat gctggatgtt atcaatggca tatgtcattt accccaacaa gactaaattt
      421 tagaaaaaac aactgccggg acaactttcg ccccctgtaa ccccaaataa gatgtcagat
      481 ttatcggcat taatttatct ccctgttgaa ttagctcctc tatcatctac ttaacaaata
      541 tcccccggac attgcaacac aaaaaccgga gccggactcc ggttttgtga agctgtcggg
      601 ttacttcatc ccgccaatat tttcccacgt cccgtcagca cgcaggattt gcagcggtct
      661 taccacgcac tgtatctgct ttttatccgc atccagtatc accacctgcg tgattaccct
      721 gtcctgctcc gggataatgc cattctcatc tgactccagg atgtctgccg gtcccagtcg
      781 cagctgtgct gtaggcgact gcacgtgttc acggccatca tgcttt
//
LOCUS       DAZKHF020000649          823 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2201S31241
            SAMN43267800-rid25106413.denovo.653, whole genome shotgun sequence.
ACCESSION   DAZKHF020000649 DAZKHF020000000
VERSION     DAZKHF020000649.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267800
            Sequence Read Archive: SRR30307263
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 823)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 823)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 823)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350581.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350581.2
            Genome Coverage       :: 151x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:21:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,652
            CDSs (total)                      :: 5,557
            Genes (coding)                    :: 5,320
            CDSs (with protein)               :: 5,320
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 237
            CDSs (without protein)            :: 237
            Pseudo Genes (ambiguous residues) :: 0 of 237
            Pseudo Genes (frameshifted)       :: 102 of 237
            Pseudo Genes (incomplete)         :: 130 of 237
            Pseudo Genes (internal stop)      :: 52 of 237
            Pseudo Genes (multiple problems)  :: 41 of 237
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..823
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267800-rid25106413.denovo.653"
                     /serotype="O 26:H11"
                     /isolate="2201S31241"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-01"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            <1..>823
                     /locus_tag="ACHOSC_RS26590"
                     /old_locus_tag="pgaptmp_005318"
     CDS             <1..>823
                     /locus_tag="ACHOSC_RS26590"
                     /old_locus_tag="pgaptmp_005318"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000119815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HGU4551034.1"
                     /translation="EDLLSWYKAKGMTSVIVHSEMDQNDLSDALMKIEHHQCDVVISV
                     NMLMEGYDHKYLTVLAIFRPYRSLNAFAQVVGRILRAIPESEITHFEIDNNGIVIFHE
                     EIGLNVMWETFQKEVERARRASVREYTFTDRDYHERSNTLAGIESDSAYVSDTDSYLD
                     DVDFNAMFEQKRNEINNSVEQQIEKIKSSGVDLPEDALEALRQTLAEKAVQKAAEIID
                     PELIEKRPHQARKLMRNILKTRTQDGVSNLLIDNGIGEKSSELYSIFNRHVPYLKT"
BASE COUNT          302 a          129 c          166 g          226 t
ORIGIN      
        1 gaagatttac tttcatggta taaagccaaa ggaatgacat cagtaattgt tcatagtgaa
       61 atggatcaaa acgacctttc tgatgctcta atgaaaatag aacatcatca atgtgacgtc
      121 gttatatctg tcaacatgct catggaaggg tatgatcata aatatctaac agtacttgct
      181 atatttagac cataccgtag cctaaacgct tttgcacaag ttgttggacg cattttacga
      241 gcgattcctg aatcagaaat tacccacttt gaaatagata acaatggaat agtcatattc
      301 catgaggaaa taggtttaaa tgtcatgtgg gaaacatttc agaaagaagt ggagagagca
      361 cgcagagcaa gtgtaagaga atatacattt acagacaggg attatcatga aagatctaat
      421 acattagctg ggatagaaag tgatagtgct tacgtaagtg atacagactc ttacctagat
      481 gatgttgact ttaatgctat gtttgaacag aagcgtaatg agatcaacaa ttctgttgag
      541 caacaaatag aaaaaataaa atcgtctgga gtagatcttc ctgaagatgc tcttgaagca
      601 ttacgccaaa cattagcaga aaaagctgtg caaaaagccg cagagataat tgatcctgag
      661 ctaattgaaa aaaggcctca tcaagcgaga aagttaatga gaaacatctt gaaaacccgc
      721 actcaggatg gtgtttctaa tttattgatt gataatggaa ttggtgaaaa aagctccgaa
      781 ctgtatagta tttttaaccg acatgtacct tatttaaaga caa
//
LOCUS       DAZKHF020000650          821 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2201S31241
            SAMN43267800-rid25106413.denovo.654, whole genome shotgun sequence.
ACCESSION   DAZKHF020000650 DAZKHF020000000
VERSION     DAZKHF020000650.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267800
            Sequence Read Archive: SRR30307263
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 821)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 821)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 821)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350581.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350581.2
            Genome Coverage       :: 151x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:21:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,652
            CDSs (total)                      :: 5,557
            Genes (coding)                    :: 5,320
            CDSs (with protein)               :: 5,320
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 237
            CDSs (without protein)            :: 237
            Pseudo Genes (ambiguous residues) :: 0 of 237
            Pseudo Genes (frameshifted)       :: 102 of 237
            Pseudo Genes (incomplete)         :: 130 of 237
            Pseudo Genes (internal stop)      :: 52 of 237
            Pseudo Genes (multiple problems)  :: 41 of 237
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..821
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267800-rid25106413.denovo.654"
                     /serotype="O 26:H11"
                     /isolate="2201S31241"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-01"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            complement(<1..553)
                     /locus_tag="ACHOSC_RS26595"
                     /old_locus_tag="pgaptmp_005319"
     CDS             complement(<1..553)
                     /locus_tag="ACHOSC_RS26595"
                     /old_locus_tag="pgaptmp_005319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000367246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4551035.1"
                     /translation="MDMIPSYESMYLTLRGIPTDSIGGEDIYFIHDPQGGWYPNRGNH
                     LYAIDALCVNIHDALANSIFDGLENYHKFLYMAPEFLSTAGLNSESVVSKETFVLFID
                     KFKGHTDVNKGLYLFDCCKIVSSIQECSKEVLQLQGEFYYTLNFEPLFFPNIEEEDGI
                     RYVTSPVVTKLFALLGFIYIRMYS"
BASE COUNT          266 a          163 c          134 g          258 t
ORIGIN      
        1 aactgtacat gcggatataa ataaatccaa gtaatgcaaa gagcttagtc acgactggag
       61 atgtaacata tcttataccg tcctcctctt caatgttagg gaaaaacaag ggttcaaaat
      121 tcaacgtgta ataaaactcc ccttgtagtt gcaacacttc cttgctgcat tcctgtatgc
      181 tacttacaat tttacagcaa tcaaaaaggt aaaggccttt atttacgtca gtatgccctt
      241 tgaatttatc aataaataaa acaaatgttt cttttgaaac tacggattca gagttcaaac
      301 ctgctgttga taaaaactca ggggccatat aaaggaactt gtggtagttt tctaatccat
      361 caaaaatgct attagctaga gcatcatgaa tattaacgca taatgcatcg atcgcataaa
      421 gatgattccc tctgttcggg taccatccac cttgaggatc atgtataaag taaatgtcct
      481 cacctccaat tgagtcagtt ggtatccctc ttaaagtcaa atacatgctt tcatatgaag
      541 gtatcatatc catgtttttc tttaattcct cttatgaatc gaaaaagtga ggtttcgtat
      601 ataggttaat ttaagcaaga taatcatact ctgaaagtaa taatttttca tatccgggtt
      661 tatcaaacgc agggaatttt atgggctttt ctttactcaa cctaacgccc gcttctggca
      721 cagagcggac tgtcagatta gattcagacc tgtactcatt agctgataca aatcagccac
      781 atatacctgc attccaaata atttcagaca gatattatcg t
//
LOCUS       DAZKHF020000651          821 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2201S31241
            SAMN43267800-rid25106413.denovo.655, whole genome shotgun sequence.
ACCESSION   DAZKHF020000651 DAZKHF020000000
VERSION     DAZKHF020000651.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267800
            Sequence Read Archive: SRR30307263
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 821)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 821)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 821)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350581.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350581.2
            Genome Coverage       :: 151x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:21:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,652
            CDSs (total)                      :: 5,557
            Genes (coding)                    :: 5,320
            CDSs (with protein)               :: 5,320
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 237
            CDSs (without protein)            :: 237
            Pseudo Genes (ambiguous residues) :: 0 of 237
            Pseudo Genes (frameshifted)       :: 102 of 237
            Pseudo Genes (incomplete)         :: 130 of 237
            Pseudo Genes (internal stop)      :: 52 of 237
            Pseudo Genes (multiple problems)  :: 41 of 237
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..821
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267800-rid25106413.denovo.655"
                     /serotype="O 26:H11"
                     /isolate="2201S31241"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-01"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            complement(352..>821)
                     /locus_tag="ACHOSC_RS26600"
                     /old_locus_tag="pgaptmp_005320"
     CDS             complement(352..>821)
                     /locus_tag="ACHOSC_RS26600"
                     /old_locus_tag="pgaptmp_005320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309587.1"
                     /note="secreted by salmonella pathogenicity island 1;
                     involved in survival of Salmonella in host cells;
                     interferes with host cell ubiquination pathway; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="E3 ubiquitin--protein ligase"
                     /protein_id="HGU4551036.1"
                     /translation="ALNSNKSDYKMIADDQQRKLACVWNPFLDGWELNAQHVDMIMGS
                     HVLKDMPLRKQAEILFCLGGVFCKYSSSDMFGTEYDSPEILRRYANGLIEQAYKTDPQ
                     VFGSVYYYNDILDRLQGRNNVFTCTAVLTDMLTEHAKESFPEIFSLYYPVAWR"
BASE COUNT          295 a          183 c          106 g          237 t
ORIGIN      
        1 acattcaaaa aaaatactga caacaaagat actcttcaga caaagttgtc atatgtaaat
       61 tttcccttaa aacgttgctc aaaaaaaata taaaaatcat atgccctaat caacatcctc
      121 aagcactacg atatatgcag tatcctattc atatattacc accatatcag acaaatcgaa
      181 tcacaacgag ttctatcgta ctctttcatc ttcgacactg taaattttaa gaacattcgg
      241 ctcatgccac taaaccaaga cataataaca acggagcaat aaatgttccc actgaacatg
      301 tgcaaaccta aaaagacagt gttacaataa tattcatcca tggtctctaa atcaacgcca
      361 cgcaacagga taatacaatg aaaatatttc aggaaaagat tcttttgcat gctccgttag
      421 catatcagtc agcacagcgg tacaagtaaa aacattattt cttccttgta gcctgtctaa
      481 aatatcattg taataataaa ctgagccaaa tacctgagga tctgttttat aagcttgttc
      541 aatcaatcca tttgcatatc tccgtagaat ctcaggagaa tcatactctg taccaaacat
      601 atcactcgat gagtatttac agaaaacccc ccctaaacaa aataatattt cagcctgttt
      661 tcttagtggc atatctttca atacatggct ccccataatc atatctacat gctgagcgtt
      721 cagttcccaa ccatcaagaa agggattcca gacacaggca agtttccttt gctgatcatc
      781 agcaatcatt ttataatctg atttattgga attaagtgcc t
//
LOCUS       DAZKHF020000652          819 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2201S31241
            SAMN43267800-rid25106413.denovo.656, whole genome shotgun sequence.
ACCESSION   DAZKHF020000652 DAZKHF020000000
VERSION     DAZKHF020000652.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267800
            Sequence Read Archive: SRR30307263
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 819)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 819)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 819)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350581.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350581.2
            Genome Coverage       :: 151x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:21:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,652
            CDSs (total)                      :: 5,557
            Genes (coding)                    :: 5,320
            CDSs (with protein)               :: 5,320
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 237
            CDSs (without protein)            :: 237
            Pseudo Genes (ambiguous residues) :: 0 of 237
            Pseudo Genes (frameshifted)       :: 102 of 237
            Pseudo Genes (incomplete)         :: 130 of 237
            Pseudo Genes (internal stop)      :: 52 of 237
            Pseudo Genes (multiple problems)  :: 41 of 237
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..819
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267800-rid25106413.denovo.656"
                     /serotype="O 26:H11"
                     /isolate="2201S31241"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-01"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            <1..>819
                     /gene="rfaL"
                     /locus_tag="ACHOSC_RS26605"
                     /old_locus_tag="pgaptmp_005321"
     CDS             <1..>819
                     /gene="rfaL"
                     /locus_tag="ACHOSC_RS26605"
                     /old_locus_tag="pgaptmp_005321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen ligase RfaL"
                     /protein_id="HGU4551037.1"
                     /translation="SLLQSPDAGASMKEIFKAIIENTLLCTIAIPIILRDEKREDVEK
                     IVFFSFISALGLRCFSELITYYKDYQQGIMPFADYRHRSISDSMVFLFPALLNLWLIK
                     SAKYRISFVVLSVIFIFLILGTLSRGAWLSVLVIGLIWILMFKQWKLLLVGVMVAIIA
                     LSVIFTHKEMTAKLTYKLQQTNSSYRYANGTQGSALDLILENPVIGYGYGNVAYKDVY
                     NKRVIDYPEWTFRQSIGPHNFALFIWFGTGLLGLVSLMMLYCAILKECIKNGVKN"
BASE COUNT          245 a          137 c          160 g          277 t
ORIGIN      
        1 tcacttcttc aatcccctga tgcaggtgct agcatgaagg aaattttcaa agctattatt
       61 gagaatactt tactatgcac aatagcaata ccgatcatat tgagagacga gaaaagagaa
      121 gatgtcgaaa aaatcgtttt cttctcattt attagtgcgt tgggcttacg ctgtttttct
      181 gaattgatta cctattataa ggactatcaa caagggataa tgccattcgc agattataga
      241 caccgtagca tttctgactc gatggtcttt ttattccctg cattgttaaa tctctggctt
      301 atcaaatcag caaaataccg catttctttt gtggttctaa gcgttatttt tatttttctg
      361 atattaggaa ctttatccag aggggcctgg ctttccgtgt tagtcattgg attaatatgg
      421 attctgatgt ttaaacaatg gaagttacta ttagtaggag taatggttgc catcattgca
      481 ttgtcggtta ttttcacaca taaggagatg accgcaaagc taacgtataa acttcaacaa
      541 actaatagtt cttatcgcta tgcaaatggt actcaaggca gcgcactcga tctaatatta
      601 gaaaatcctg ttattggtta tggttacggt aacgttgcat ataaagatgt ctataataaa
      661 cgtgtcattg attatccaga atggaccttt agacaatcaa tagggccaca taattttgcg
      721 ctattcatct ggtttggcac tggtttatta gggctggtaa gtcttatgat gctatactgt
      781 gcaatattga aagagtgtat aaaaaatggc gtcaagaat
//
LOCUS       DAZKHF020000653          818 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2201S31241
            SAMN43267800-rid25106413.denovo.657, whole genome shotgun sequence.
ACCESSION   DAZKHF020000653 DAZKHF020000000
VERSION     DAZKHF020000653.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267800
            Sequence Read Archive: SRR30307263
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 818)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 818)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 818)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350581.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350581.2
            Genome Coverage       :: 151x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:21:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,652
            CDSs (total)                      :: 5,557
            Genes (coding)                    :: 5,320
            CDSs (with protein)               :: 5,320
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 237
            CDSs (without protein)            :: 237
            Pseudo Genes (ambiguous residues) :: 0 of 237
            Pseudo Genes (frameshifted)       :: 102 of 237
            Pseudo Genes (incomplete)         :: 130 of 237
            Pseudo Genes (internal stop)      :: 52 of 237
            Pseudo Genes (multiple problems)  :: 41 of 237
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..818
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267800-rid25106413.denovo.657"
                     /serotype="O 26:H11"
                     /isolate="2201S31241"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-01"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            20..>818
                     /locus_tag="ACHOSC_RS26610"
                     /old_locus_tag="pgaptmp_005322"
     CDS             20..>818
                     /locus_tag="ACHOSC_RS26610"
                     /old_locus_tag="pgaptmp_005322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HGU4551038.1"
                     /translation="MANLYDLKKFDLNLLVIFECIYQHLSISKAAESLYITPSAVSQS
                     LQRLRAQFNDPLFIRSGKGIAPTTTGLNLHHHLEKNLKGLEQTINIVNKSELKKNFII
                     YGPQLISCSNNSMLIRCLRQDASVEIECHDILMSAENAEELLVHRKADLVITQMPVIS
                     RSVICMPLHTIRNTLICSNKHPRITDNSTYEQIMAEEFTQLISKSAGVDDIQMEIDEK
                     FMNRKISFRGSSLLTIINSIAVTDLLGIVPYELYNSYRDFLNLKEIKL"
BASE COUNT          259 a          177 c          148 g          234 t
ORIGIN      
        1 aatataaccg gagtagtgta tggccaatct ctacgacttg aaaaagttcg acttaaacct
       61 tcttgtcata tttgagtgta tttaccagca tttaagcatt agcaaagctg ctgaatcact
      121 atatatcacc ccatcagccg tgagccagtc gttacaacgc ttacgtgcgc aatttaatga
      181 ccctttattc attcgctcag gaaaaggtat cgcgccgacg acaacagggt tgaatctgca
      241 tcaccatctt gagaaaaacc tcaaaggcct tgaacaaaca attaatatag tcaataagtc
      301 cgaactaaag aaaaacttta ttatttacgg ccctcagctc atttcttgct ctaataatag
      361 tatgctgatc cgctgcctac ggcaagatgc ctctgtcgag attgagtgcc atgatatcct
      421 catgtcagca gagaatgctg aagaactgtt ggttcaccgc aaagccgatc tggtgatcac
      481 ccaaatgcca gtgatcagcc gttccgtcat ttgcatgcca ttgcatacaa ttcgcaatac
      541 tctaatttgt agtaataagc atccgcgaat tactgacaat agcacttatg aacaaattat
      601 ggcagaagag ttcacccaat taatatccaa atcagcagga gttgatgata ttcaaatgga
      661 aatagacgag aagtttatga atcgcaaaat ttcatttcgt ggttcctctt tgctgacaat
      721 cattaatagc attgcagtta cagatttact gggaatagtc ccctatgagc tttataattc
      781 ctaccgtgac tttcttaatt taaaagagat aaaactag
//
LOCUS       DAZKHF020000654          818 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2201S31241
            SAMN43267800-rid25106413.denovo.658, whole genome shotgun sequence.
ACCESSION   DAZKHF020000654 DAZKHF020000000
VERSION     DAZKHF020000654.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267800
            Sequence Read Archive: SRR30307263
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 818)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 818)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 818)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350581.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350581.2
            Genome Coverage       :: 151x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:21:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,652
            CDSs (total)                      :: 5,557
            Genes (coding)                    :: 5,320
            CDSs (with protein)               :: 5,320
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 237
            CDSs (without protein)            :: 237
            Pseudo Genes (ambiguous residues) :: 0 of 237
            Pseudo Genes (frameshifted)       :: 102 of 237
            Pseudo Genes (incomplete)         :: 130 of 237
            Pseudo Genes (internal stop)      :: 52 of 237
            Pseudo Genes (multiple problems)  :: 41 of 237
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..818
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267800-rid25106413.denovo.658"
                     /serotype="O 26:H11"
                     /isolate="2201S31241"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-01"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            43..374
                     /gene="sctI"
                     /locus_tag="ACHOSC_RS26615"
                     /old_locus_tag="pgaptmp_005323"
                     /pseudo
     CDS             43..374
                     /gene="sctI"
                     /locus_tag="ACHOSC_RS26615"
                     /old_locus_tag="pgaptmp_005323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311744.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system inner rod subunit
                     SctI"
     gene            371..>818
                     /gene="sctJ"
                     /locus_tag="ACHOSC_RS26620"
                     /old_locus_tag="pgaptmp_005324"
     CDS             371..>818
                     /gene="sctJ"
                     /locus_tag="ACHOSC_RS26620"
                     /old_locus_tag="pgaptmp_005324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009306 - protein secretion [Evidence IEA];
                     GO_process: GO:0030254 - protein secretion by the type III
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system inner membrane ring
                     lipoprotein SctJ"
                     /protein_id="HGU4551039.1"
                     /translation="MKYISLLLFILLLCGCKQQELLNHLDQQQANDVLAVLQRHNINA
                     EKKDQGKTGFSIFVEPTDFASAVDWLKIYNLPGKPDIQISQMFPADALVSSPRAEKAR
                     LYSAIEQRLEQSLKIMDGIISSRVHVSYDVDNGDSGKTALPIHISVL"
BASE COUNT          259 a          147 c          167 g          245 t
ORIGIN      
        1 caatcataca aaacttccgc taaactagat aaggatttta ttatgttagt aagcaatatg
       61 ccacctattg atagagctga gcaatcaact gctcatgaaa tacagcaagc gaaagtgatt
      121 gatttaaatg accgggttct caatcttgac aacccggatg ataaaatgat ctccgctttt
      181 gccaactatg ctgtacaaac tgaaaattgg cagcaaaatg cgttacaagc attaaggtct
      241 gataaagagg ggttaactcc tgaaaaatta ttagtcttgc aggatcatgt gcttaattat
      301 aatgtcgagg tttcattggt ggaacattag caagaaagat tgtcgctgca gttgagacgt
      361 tgacccgctc atgaaatata tatcattatt attgtttatc ctgctgttgt gcggctgcaa
      421 gcagcaggaa ttattgaacc atcttgatca acagcaagct aatgatgtcc ttgctgtttt
      481 acaaagacat aatattaatg ctgaaaagaa ggatcaaggc aaaacaggat tttcaatatt
      541 tgtcgaacca acggattttg cttctgcggt ggattggcta aaaatctaca atctaccggg
      601 taagcctgat attcaaatat cgcagatgtt ccctgcagat gcactcgttt catctccacg
      661 agcggaaaaa gcaaggctat attctgctat tgagcagcgg ttagagcaat ctctcaaaat
      721 tatggatggc attatttctt cgagagtaca tgttagttac gatgttgata atggcgatag
      781 tggtaaaact gctttaccaa ttcatatttc tgtacttg
//
LOCUS       DAZKHF020000655          813 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2201S31241
            SAMN43267800-rid25106413.denovo.659, whole genome shotgun sequence.
ACCESSION   DAZKHF020000655 DAZKHF020000000
VERSION     DAZKHF020000655.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267800
            Sequence Read Archive: SRR30307263
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 813)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 813)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 813)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350581.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350581.2
            Genome Coverage       :: 151x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:21:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,652
            CDSs (total)                      :: 5,557
            Genes (coding)                    :: 5,320
            CDSs (with protein)               :: 5,320
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 237
            CDSs (without protein)            :: 237
            Pseudo Genes (ambiguous residues) :: 0 of 237
            Pseudo Genes (frameshifted)       :: 102 of 237
            Pseudo Genes (incomplete)         :: 130 of 237
            Pseudo Genes (internal stop)      :: 52 of 237
            Pseudo Genes (multiple problems)  :: 41 of 237
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..813
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267800-rid25106413.denovo.659"
                     /serotype="O 26:H11"
                     /isolate="2201S31241"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-01"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            192..794
                     /gene="fimB"
                     /locus_tag="ACHOSC_RS26625"
                     /old_locus_tag="pgaptmp_005325"
     CDS             192..794
                     /gene="fimB"
                     /locus_tag="ACHOSC_RS26625"
                     /old_locus_tag="pgaptmp_005325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbria switch DNA invertase FimB"
                     /protein_id="HGU4551040.1"
                     /translation="MKNKADNKKRNFLTHSEIESLLKAANTGPHAARNYCLTLLCFIH
                     GFRASEICRLRISDIDLKAKCIYIHRLKKGFSTTHPLLNKEVQALKNWLSIRTSYPHA
                     ESEWVFLSRKGNPLSRQQFYHIISTSGGNAGLSLEIHPHMLRHSCGFALANMGIDTRL
                     IQDYLGHRNIRHTVWYTASNAGRFYGIWDRARGRQRHAVL"
BASE COUNT          234 a          158 c          182 g          239 t
ORIGIN      
        1 catagcgttt gtatggcaac gttattataa gtaactgttg ctactccatt taagttcact
       61 cagaagaact ggtccactta cgttagttat taagcaaacg ttcgctttta taaacataat
      121 caggataaaa atgttggatt attgctaacc cagcacagct agtgcgcgtc tgtaattata
      181 agggaaaaac gatgaagaat aaggctgata acaaaaaaag gaacttcctg acccatagtg
      241 aaatcgaatc actccttaaa gcagcaaata ccgggcctca tgcagcacgt aattattgtc
      301 tgactttgct ttgttttatt catggtttcc gggcgagtga aatttgtcga ttgaggattt
      361 cggatattga tcttaaggca aagtgtatat atatccatcg attaaaaaaa ggcttttcaa
      421 caacgcaccc gctattgaac aaagaagttc aggctttaaa aaactggttg agtatccgta
      481 cttcgtaccc gcatgctgag agcgaatggg tatttttatc gcgtaaggga aatccgcttt
      541 ctcggcaaca gttttaccat attatctcga cttccggtgg taatgccggg ttgtcactgg
      601 agattcatcc gcacatgtta cgccattcgt gtggttttgc tttggcgaat atgggaatag
      661 atacgcgact tatccaggat tatcttgggc atcgcaatat tcgtcatact gtctggtata
      721 ccgccagcaa tgcagggcgt ttttacggca tctgggatag agccagagga cgacagcgtc
      781 acgctgtttt atagagatag ttgagatacc agg
//
LOCUS       DAZKHF020000656          811 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2201S31241
            SAMN43267800-rid25106413.denovo.660, whole genome shotgun sequence.
ACCESSION   DAZKHF020000656 DAZKHF020000000
VERSION     DAZKHF020000656.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267800
            Sequence Read Archive: SRR30307263
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 811)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 811)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 811)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350581.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350581.2
            Genome Coverage       :: 151x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:21:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,652
            CDSs (total)                      :: 5,557
            Genes (coding)                    :: 5,320
            CDSs (with protein)               :: 5,320
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 237
            CDSs (without protein)            :: 237
            Pseudo Genes (ambiguous residues) :: 0 of 237
            Pseudo Genes (frameshifted)       :: 102 of 237
            Pseudo Genes (incomplete)         :: 130 of 237
            Pseudo Genes (internal stop)      :: 52 of 237
            Pseudo Genes (multiple problems)  :: 41 of 237
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..811
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267800-rid25106413.denovo.660"
                     /serotype="O 26:H11"
                     /isolate="2201S31241"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-01"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            <1..642
                     /locus_tag="ACHOSC_RS26630"
                     /old_locus_tag="pgaptmp_005326"
     CDS             <1..642
                     /locus_tag="ACHOSC_RS26630"
                     /old_locus_tag="pgaptmp_005326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016244183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4551041.1"
                     /translation="RLALQKGTGAGNISALNKVMKGTLVTSLALVAVTTGPDMIKMLR
                     GRISGTQFIRNLAVASSGVAGGAVGSVAGGILFSPLGPFGALTGRVVGGVLGGMIASA
                     VSGKIAGALVEEDRVKILAMIQEQVTWLAGSFLLTGHEIENLNENLARVIDQNALEII
                     FAAGIQQRAATNMLIKPLVVSIIRQRPVMEYDASHLGNMVNRLEEALPPELPA"
     gene            718..>811
                     /locus_tag="ACHOSC_RS26635"
                     /old_locus_tag="pgaptmp_005327"
     CDS             718..>811
                     /locus_tag="ACHOSC_RS26635"
                     /old_locus_tag="pgaptmp_005327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4551042.1"
                     /translation="MIHLFKTCMITAFILGLTWSAPLRAQDQRYI"
BASE COUNT          190 a          197 c          227 g          197 t
ORIGIN      
        1 cgactggctc ttcagaaggg gaccggtgca ggaaatatca gtgccctgaa caaagtgatg
       61 aagggtacgc tggtgacatc tctggcactg gtagctgtca caaccggccc tgacatgatc
      121 aaaatgttgc ggggacggat ctccggtacg cagttcatca ggaatcttgc cgtggcatct
      181 tccggtgtgg caggtggtgc tgtcgggtca gtggcgggcg gaatactgtt cagtccactg
      241 ggaccatttg gtgcactgac agggcgtgtg gttggcggtg ttctgggggg aatgattgcc
      301 tccgctgtat caggaaaaat tgccggagcg ctggttgaag aagatcgcgt caaaattctg
      361 gcaatgattc aggagcaggt gacatggctt gccggcagtt tcctgctgac cggacatgag
      421 attgaaaatc tgaacgagaa tctggcccgt gttatcgatc agaatgctct ggagatcatt
      481 ttcgccgccg gtatacaaca acgggccgcg accaatatgt taatcaaacc actggtggtc
      541 agtatcatca ggcaacgccc cgtcatggaa tatgatgcat cccatctcgg caatatggtt
      601 aaccgactgg aagaagcatt acccccggag ttaccggcat aacggctatt ctgcgccttt
      661 tacgctttat cacctgattt gtcagtgtca tcgtcatgac tcagagaggt aaataccatg
      721 attcacctgt tcaaaacctg catgattacc gccttcattc tggggttaac gtggtctgcc
      781 ccactccggg cacaggatca acgttacatc a
//
LOCUS       DAZKHF020000657          808 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2201S31241
            SAMN43267800-rid25106413.denovo.661, whole genome shotgun sequence.
ACCESSION   DAZKHF020000657 DAZKHF020000000
VERSION     DAZKHF020000657.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267800
            Sequence Read Archive: SRR30307263
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 808)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 808)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 808)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350581.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350581.2
            Genome Coverage       :: 151x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:21:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,652
            CDSs (total)                      :: 5,557
            Genes (coding)                    :: 5,320
            CDSs (with protein)               :: 5,320
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 237
            CDSs (without protein)            :: 237
            Pseudo Genes (ambiguous residues) :: 0 of 237
            Pseudo Genes (frameshifted)       :: 102 of 237
            Pseudo Genes (incomplete)         :: 130 of 237
            Pseudo Genes (internal stop)      :: 52 of 237
            Pseudo Genes (multiple problems)  :: 41 of 237
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..808
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267800-rid25106413.denovo.661"
                     /serotype="O 26:H11"
                     /isolate="2201S31241"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-01"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            <1..>808
                     /locus_tag="ACHOSC_RS26640"
                     /old_locus_tag="pgaptmp_005328"
     CDS             <1..>808
                     /locus_tag="ACHOSC_RS26640"
                     /old_locus_tag="pgaptmp_005328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="terminase large subunit"
                     /protein_id="HGU4551043.1"
                     /translation="SPLYTFDREVVERFIAFSRVCPHVKGPMRGRPIELEPWQQFAFA
                     CILGFKVKATGRRKYTSAFIEVPRKNAKSTTAAILANWFLIMENGQQDIYTAAVSRDQ
                     ARIVFDDARQMCLLSRPLRRRVNIQAHKVMHPKSNSLLKPLAAKAATIEGTNPSLSIV
                     DEYHLHSDNGVYSALELGMGARPEGLLFAITTSGSNVVSACKQHYDYCCQILDGEEVN
                     DSIFVLIYELDDENEVDDPAMWIKANPNIDVSVDREKLASTIQKARGIPS"
BASE COUNT          193 a          211 c          226 g          178 t
ORIGIN      
        1 aaagccccct ttacacgttc gatcgtgagg ttgtggagcg gtttattgcc ttttccaggg
       61 tgtgcccgca cgtaaaaggg ccgatgcgtg gcagacccat tgagctggag ccgtggcagc
      121 agttcgcctt tgcgtgcatc ctcggcttta aggttaaggc caccggacgg cgcaaataca
      181 cgagcgcctt tatcgaagtg ccgcgcaaaa atgcgaaatc cacgaccgcc gcgattctgg
      241 ctaactggtt tctgattatg gagaacgggc agcaggatat ttacaccgca gcggtgagcc
      301 gtgaccaggc gcggatcgtt tttgatgatg cgcgtcagat gtgcctttta tcccgaccgt
      361 tacgcaggcg ggtgaatatt caggcccaca aggtgatgca cccgaaaagc aacagcctgt
      421 taaagccgct ggcagcaaaa gcggcaacca tcgagggcac aaacccaagc ctttccatcg
      481 tggacgaata ccacctacac tccgataacg gcgtttattc cgcgcttgag ctggggatgg
      541 gcgcacgtcc cgaagggctg ttatttgcca ttaccacatc gggcagtaac gtcgtttcag
      601 cctgtaaaca gcactatgat tattgctgcc agatcctgga cggcgaagag gtcaacgatt
      661 caatttttgt gctgatttac gagctggacg acgaaaacga ggttgatgat ccggcgatgt
      721 ggataaaggc taaccctaac atcgatgttt ccgtggatcg tgaaaaactg gcctcaacca
      781 tccagaaagc gcggggtatt ccgtcgca
//
LOCUS       DAZKHF020000658          808 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2201S31241
            SAMN43267800-rid25106413.denovo.662, whole genome shotgun sequence.
ACCESSION   DAZKHF020000658 DAZKHF020000000
VERSION     DAZKHF020000658.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267800
            Sequence Read Archive: SRR30307263
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 808)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 808)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 808)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350581.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350581.2
            Genome Coverage       :: 151x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:21:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,652
            CDSs (total)                      :: 5,557
            Genes (coding)                    :: 5,320
            CDSs (with protein)               :: 5,320
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 237
            CDSs (without protein)            :: 237
            Pseudo Genes (ambiguous residues) :: 0 of 237
            Pseudo Genes (frameshifted)       :: 102 of 237
            Pseudo Genes (incomplete)         :: 130 of 237
            Pseudo Genes (internal stop)      :: 52 of 237
            Pseudo Genes (multiple problems)  :: 41 of 237
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..808
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267800-rid25106413.denovo.662"
                     /serotype="O 26:H11"
                     /isolate="2201S31241"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-01"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            complement(<1..461)
                     /locus_tag="ACHOSC_RS26645"
                     /old_locus_tag="pgaptmp_005329"
     CDS             complement(<1..461)
                     /locus_tag="ACHOSC_RS26645"
                     /old_locus_tag="pgaptmp_005329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001448330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004842 - ubiquitin-protein transferase
                     activity [Evidence IEA];
                     GO_process: GO:0044403 - biological process involved in
                     symbiotic interaction [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="T3SS effector NleG family protein"
                     /protein_id="HGU4551044.1"
                     /translation="MPINLTPYLTASGGLGEIPQDTLSGIRILAFNGGAQVQMGSTIV
                     TIRSVFLGFFMGSVSPEGLSERALRTALNNVNRLERDLNGGLSGRQILASIPSPYASP
                     PRPSVMQTELVLEQIEKCSFNVNSSSLRATEEALTCPITLCIPEHGVIMRNA"
     gene            complement(671..>808)
                     /locus_tag="ACHOSC_RS26650"
                     /old_locus_tag="pgaptmp_005330"
     CDS             complement(671..>808)
                     /locus_tag="ACHOSC_RS26650"
                     /old_locus_tag="pgaptmp_005330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311516.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail fiber C-terminal domain-containing
                     protein"
                     /protein_id="HGU4551045.1"
                     /translation="IIPEQARVITQVVILDADKKQIQCVVRPLQILRADGTWENIGWM
                     K"
BASE COUNT          232 a          206 c          141 g          229 t
ORIGIN      
        1 gcatttctca taatcacacc atgctcaggg atgcataatg taatcgggca tgttaatgcc
       61 tcttcagtcg ccctcagaga tgacgagtta acattaaatg agcatttttc tatttgctct
      121 aacaccaact ctgtttgcat tacagaagga cgaggtggag aagcatacgg gctgggtata
      181 cttgctaaaa tctggcggcc ggatagccct ccattaaggt cgcgttcaag acgattaaca
      241 ttattcaatg cagttcgtaa agctctctct gataatcctt cagggcttac acttcccata
      301 aagaagccta aaaagacaga acgtattgta acaatagtgc tccccatctg aacctgagct
      361 cctccgttaa aagccagtat tcgtatgcca gaaagagtat cctgcggtat ttcccccagt
      421 cccccactcg ctgttagata tggtgttaaa tttattggca taataaaacc cccattaact
      481 tcattaacat tttacttgat ttcctgttga agcatcttac ttatcacaat gctttcacac
      541 accattatca gtcttgcaaa agataagact ccattatcaa gccaaaataa atttaacttc
      601 attaacaaac atcaccatga catgacaaca aaaaaccgga gccagactcc ggttttttga
      661 agctgtcggg ttacttcatc cagccaatat tttcccacgt cccgtcagca cgcagaattt
      721 gcagcggtct taccacgcac tgtatctgct ttttatccgc atccagtatc accacctgcg
      781 tgattaccct ggcctgctcc gggataat
//
LOCUS       DAZKHF020000659          806 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2201S31241
            SAMN43267800-rid25106413.denovo.663, whole genome shotgun sequence.
ACCESSION   DAZKHF020000659 DAZKHF020000000
VERSION     DAZKHF020000659.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267800
            Sequence Read Archive: SRR30307263
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 806)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 806)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 806)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350581.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350581.2
            Genome Coverage       :: 151x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:21:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,652
            CDSs (total)                      :: 5,557
            Genes (coding)                    :: 5,320
            CDSs (with protein)               :: 5,320
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 237
            CDSs (without protein)            :: 237
            Pseudo Genes (ambiguous residues) :: 0 of 237
            Pseudo Genes (frameshifted)       :: 102 of 237
            Pseudo Genes (incomplete)         :: 130 of 237
            Pseudo Genes (internal stop)      :: 52 of 237
            Pseudo Genes (multiple problems)  :: 41 of 237
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..806
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267800-rid25106413.denovo.663"
                     /serotype="O 26:H11"
                     /isolate="2201S31241"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-01"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            <1..>806
                     /locus_tag="ACHOSC_RS26655"
                     /old_locus_tag="pgaptmp_005331"
     CDS             <1..>806
                     /locus_tag="ACHOSC_RS26655"
                     /old_locus_tag="pgaptmp_005331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000833619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helicase RepA family protein"
                     /protein_id="HGU4551046.1"
                     /translation="AAGVTDANYYPEGMHSQKRENWREYLERERGKKPGEEKHHQRKT
                     TLPMSVGSAGYDTQLDYVVKGIIPAVSLCSIYGASGSYKSFLAGSWACHVSTGRQWGG
                     RRVAHGAVLYVVGEGGIGVPRRVKAWEVVHGEQVKNLYLVNRPIFPAAPLDVEEMVIA
                     ARQVERETGKPVRMIILDTLARCFGGNDENDSRDMGAFIRGCDELKRRTGATVLVVHH
                     SGKDETKGARGSSAFRASLDAEYRIRREDAGSEALVISCTKMKDAEELKEA"
BASE COUNT          191 a          179 c          273 g          163 t
ORIGIN      
        1 gcggcgggtg taactgatgc gaattattac cccgaaggga tgcacagcca gaagcgggag
       61 aactggcgcg aatatctgga acgggagcgg gggaaaaagc caggggaaga aaaacaccac
      121 cagcgaaaaa ccacgctacc aatgagcgtg ggatctgccg gatacgacac acaacttgat
      181 tacgtggtta aggggattat tccggcggta tcgctgtgca gcatatacgg ggcgagcggt
      241 tcatataaat cgttccttgc gggttcgtgg gcgtgtcacg tttccacggg ccgccagtgg
      301 ggaggccgca gggtggcgca tggcgctgtt ctctatgtgg ttggtgaagg cggtatcggc
      361 gttccgcgtc gtgtaaaagc ctgggaggtt gtgcacggtg agcaggtgaa aaatctgtat
      421 ctggtaaacc gtcccatctt tccggctgcc ccgcttgatg ttgaggaaat ggttatcgct
      481 gcccgtcagg tggaacggga aacgggtaaa ccggttcgaa tgattattct ggatacgctg
      541 gcgcgctgct ttggcggcaa tgatgaaaat gattcccgtg atatgggggc gtttatccgt
      601 gggtgtgacg aactgaaacg gcgcacaggg gcaacggtgc tggtggttca ccattccggc
      661 aaggatgaaa caaagggggc gcgtggttcc agtgcatttc gtgcatcact ggacgccgaa
      721 taccggatac gcagggagga cgcaggcagc gaagcgctgg ttatctcatg taccaaaatg
      781 aaggacgcgg aggaactcaa agaagc
//
LOCUS       DAZKHF020000660          805 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2201S31241
            SAMN43267800-rid25106413.denovo.664, whole genome shotgun sequence.
ACCESSION   DAZKHF020000660 DAZKHF020000000
VERSION     DAZKHF020000660.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267800
            Sequence Read Archive: SRR30307263
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 805)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 805)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 805)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350581.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350581.2
            Genome Coverage       :: 151x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:21:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,652
            CDSs (total)                      :: 5,557
            Genes (coding)                    :: 5,320
            CDSs (with protein)               :: 5,320
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 237
            CDSs (without protein)            :: 237
            Pseudo Genes (ambiguous residues) :: 0 of 237
            Pseudo Genes (frameshifted)       :: 102 of 237
            Pseudo Genes (incomplete)         :: 130 of 237
            Pseudo Genes (internal stop)      :: 52 of 237
            Pseudo Genes (multiple problems)  :: 41 of 237
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..805
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267800-rid25106413.denovo.664"
                     /serotype="O 26:H11"
                     /isolate="2201S31241"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-01"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            <1..571
                     /locus_tag="ACHOSC_RS26660"
                     /old_locus_tag="pgaptmp_005332"
     CDS             <1..571
                     /locus_tag="ACHOSC_RS26660"
                     /old_locus_tag="pgaptmp_005332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000491542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="secretion protein EspS"
                     /protein_id="HGU4551047.1"
                     /translation="LQSSNSDVVAAAAISITATEMKTILPDDLTPGKYNKIYLSGHGS
                     AGLPLLKCGDEFLSPSDIVDRIVQHNLHEIDDIRLTSCNSANIIKNKDFSPDEIEKSA
                     NMNNGWLARALFGQKRSLAEHVYAEFERRGINVSISGYHGTGVFYVPEHGKPTTHLRS
                     TTVPATPEHTVRRSDYRATLGRTQPIDIE"
BASE COUNT          255 a          166 c          164 g          220 t
ORIGIN      
        1 tctacaatca agtaatagcg atgttgttgc tgctgcagct ataagtatta cagcgacaga
       61 gatgaaaacc atcctgccag atgatttaac accaggaaaa tacaacaaaa tttatctgtc
      121 tgggcatggt tctgctggtc tacctcttct taagtgcgga gatgaatttt tatcaccgtc
      181 agatattgtc gaccgcattg ttcaacataa tcttcatgaa atagatgata tcagattaac
      241 atcctgtaac tcagccaaca taataaaaaa caaagacttc tctcctgatg aaatagaaaa
      301 atccgcaaat atgaataacg gctggttggc cagggcatta tttggtcaaa agaggtcttt
      361 agcagaacac gtctatgccg agtttgaacg tcgcggaatt aacgtttcta tatcaggtta
      421 ccatggcact ggcgtttttt atgtaccaga gcatggtaaa ccaacaacgc atctacgctc
      481 cacaactgtg cctgcaacac ctgaacatac tgtaagaaga agcgactaca gagccacttt
      541 gggtagaact caacccatag atattgagta attgagccag catatacact tgatggttag
      601 gaaacagaat cattgtttta aacatttcct ggccattaat attgtcggcg ggagcatatc
      661 caggactggc cggcaaaccg ggtacgcgat ctgttgccat ggaaagttga tctgagcgct
      721 cagtaaatat caatacggtt ctggcgagcc gcttacgatg catgagaatc actaatagta
      781 tgtaattatt acattttatt tacaa
//
LOCUS       DAZKHF020000661          803 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2201S31241
            SAMN43267800-rid25106413.denovo.665, whole genome shotgun sequence.
ACCESSION   DAZKHF020000661 DAZKHF020000000
VERSION     DAZKHF020000661.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267800
            Sequence Read Archive: SRR30307263
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 803)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 803)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 803)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350581.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350581.2
            Genome Coverage       :: 151x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:21:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,652
            CDSs (total)                      :: 5,557
            Genes (coding)                    :: 5,320
            CDSs (with protein)               :: 5,320
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 237
            CDSs (without protein)            :: 237
            Pseudo Genes (ambiguous residues) :: 0 of 237
            Pseudo Genes (frameshifted)       :: 102 of 237
            Pseudo Genes (incomplete)         :: 130 of 237
            Pseudo Genes (internal stop)      :: 52 of 237
            Pseudo Genes (multiple problems)  :: 41 of 237
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..803
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267800-rid25106413.denovo.665"
                     /serotype="O 26:H11"
                     /isolate="2201S31241"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-01"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            <1..>803
                     /locus_tag="ACHOSC_RS26665"
                     /old_locus_tag="pgaptmp_005333"
     CDS             <1..>803
                     /locus_tag="ACHOSC_RS26665"
                     /old_locus_tag="pgaptmp_005333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000833619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helicase RepA family protein"
                     /protein_id="HGU4551048.1"
                     /translation="AAGVQDANYYPDGMHSQKRENWREYLERERGEKQEEKGHQRKTK
                     LPMSVGSAGYDTQLDYVVKGIIPAVSLCSIYGASGSYKSFLAGSWACHVSTGRQWGGR
                     RVAHGAVLYVVGEGGIGVPRRVKAWEVVHGEQVKNLYLVNRPIFPAAPLDVDEMVIAA
                     RQVERETGKPVRMIILDTLARCFGGNDENDSRDMGTFIRGCDELKRRTGATVLVVHHS
                     GKDETKGARGSSAFRASLDAEYRIRREDAGSEALVISCTKMKDAEELKEA"
BASE COUNT          184 a          181 c          270 g          168 t
ORIGIN      
        1 gcggcaggtg tacaggatgc gaattactac cccgatggga tgcacagcca gaagcgggag
       61 aactggcgcg aatatctgga acgggagcgc ggggaaaagc aggaagaaaa aggccaccag
      121 cgcaaaacaa agctaccgat gagcgttggt tctgccggat atgacacgca actggattac
      181 gtggttaagg ggattattcc ggcggtatcg ctttgcagca tatacggggc gagcggttcc
      241 tataaatcgt tccttgcggg ttcgtgggcg tgtcacgttt ccacgggccg ccagtgggga
      301 ggccgcaggg tggcgcatgg cgctgttctc tatgtggttg gtgaaggcgg tatcggcgtt
      361 ccgcgtcgtg taaaagcctg ggaggttgtg cacggtgagc aggtgaaaaa tctttacctt
      421 gtaaaccgtc ccatctttcc ggctgccccg cttgatgttg atgaaatggt tatcgctgcc
      481 cgtcaggtgg agcgggaaac gggtaaacct gttcgaatga ttattctgga tacgctggcg
      541 cgctgctttg gcggcaatga tgaaaatgac tcccgtgata tggggacgtt tatccgtggg
      601 tgtgacgaac tgaaacgacg cacaggggcc acggtgctgg tggttcacca ttccggcaag
      661 gatgagacga aaggcgcgcg cggttccagt gcatttcgtg cttcgctgga tgctgaatac
      721 cggatacgca gggaggacgc aggaagcgaa gcgctggtta tctcatgcac caaaatgaag
      781 gacgcggagg aactcaaaga agc
//
LOCUS       DAZKHF020000662          801 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2201S31241
            SAMN43267800-rid25106413.denovo.666, whole genome shotgun sequence.
ACCESSION   DAZKHF020000662 DAZKHF020000000
VERSION     DAZKHF020000662.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267800
            Sequence Read Archive: SRR30307263
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 801)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 801)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 801)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350581.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350581.2
            Genome Coverage       :: 151x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:21:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,652
            CDSs (total)                      :: 5,557
            Genes (coding)                    :: 5,320
            CDSs (with protein)               :: 5,320
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 237
            CDSs (without protein)            :: 237
            Pseudo Genes (ambiguous residues) :: 0 of 237
            Pseudo Genes (frameshifted)       :: 102 of 237
            Pseudo Genes (incomplete)         :: 130 of 237
            Pseudo Genes (internal stop)      :: 52 of 237
            Pseudo Genes (multiple problems)  :: 41 of 237
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..801
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267800-rid25106413.denovo.666"
                     /serotype="O 26:H11"
                     /isolate="2201S31241"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-01"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            39..407
                     /locus_tag="ACHOSC_RS26670"
                     /old_locus_tag="pgaptmp_005334"
     CDS             39..407
                     /locus_tag="ACHOSC_RS26670"
                     /old_locus_tag="pgaptmp_005334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2528 family protein"
                     /protein_id="HGU4551049.1"
                     /translation="MSNIKKYIIDYDWKTSIEIEIDHDLMTEEKLHQINNFWSDSEYR
                     LNKHGSVLNAVLIMLAQHALLIAISSDLNAYGVVCEFDWNDGNGQEGWPPMDGSEGIR
                     ITDIDTSGIFDPDDMTIKAA"
     gene            480..644
                     /locus_tag="ACHOSC_RS26675"
                     /old_locus_tag="pgaptmp_005335"
     CDS             480..644
                     /locus_tag="ACHOSC_RS26675"
                     /old_locus_tag="pgaptmp_005335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protease FtsH-inhibitory lysogeny factor CIII"
                     /protein_id="HGU4551050.1"
                     /translation="MQYAIAGWPVAGCPSESLLERITRKLRDGWKRLIDILNQPGVPK
                     NGSNNYGYPD"
     gene            613..777
                     /gene="kil"
                     /locus_tag="ACHOSC_RS26680"
                     /old_locus_tag="pgaptmp_005336"
     CDS             613..777
                     /gene="kil"
                     /locus_tag="ACHOSC_RS26680"
                     /old_locus_tag="pgaptmp_005336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000372922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="host cell division inhibitory peptide Kil"
                     /protein_id="HGU4551051.1"
                     /translation="MDQTIMAIQTKFTIATFIGDEKMFREAVDAYKKWILIQKLRSSK
                     SIHYPPFLFS"
BASE COUNT          253 a          163 c          169 g          216 t
ORIGIN      
        1 aatgtgaggg cgattttttt ctgtgaggat atgaacagat gtcaaacatc aaaaaataca
       61 tcattgatta cgactggaaa acatcaatag aaattgaaat cgaccatgac ttaatgacag
      121 aggaaaaact tcaccagatt aataatttct ggtcagactc tgaataccga ctcaataaac
      181 acggctctgt attaaatgct gtattaatca tgctggcgca acatgctctg cttatagcaa
      241 tttcaagcga cttaaatgca tatggtgttg tgtgtgagtt cgactggaat gatggaaatg
      301 gtcaggaagg atggcctcca atggatggta gcgaaggaat aagaattacc gatatcgata
      361 catcaggaat atttgatcca gatgatatga ctatcaaagc cgcctgagcg cggcgttacc
      421 gcataccaat tacgcttcac tcgaggcgtt tttcgttatg tataaataag gagcacacca
      481 tgcaatatgc cattgcaggg tggcctgttg ctggctgccc ttccgaatct ttacttgaac
      541 gaattacccg taaattacgt gacggatgga aacgccttat cgacatactt aatcagccag
      601 gagtcccgaa aaatggatca aacaattatg gctatccaga ctaaattcac tatcgccact
      661 tttattggcg atgaaaagat gtttcgtgag gccgtcgacg cttataaaaa atggatatta
      721 atacagaaac tgagatcaag caaaagcatt cactaccccc ctttcctgtt ttcctaatca
      781 gcctggcatt tcgcgggcga t
//
LOCUS       DAZKHF020000663          796 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2201S31241
            SAMN43267800-rid25106413.denovo.667, whole genome shotgun sequence.
ACCESSION   DAZKHF020000663 DAZKHF020000000
VERSION     DAZKHF020000663.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267800
            Sequence Read Archive: SRR30307263
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 796)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 796)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 796)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350581.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350581.2
            Genome Coverage       :: 151x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:21:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,652
            CDSs (total)                      :: 5,557
            Genes (coding)                    :: 5,320
            CDSs (with protein)               :: 5,320
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 237
            CDSs (without protein)            :: 237
            Pseudo Genes (ambiguous residues) :: 0 of 237
            Pseudo Genes (frameshifted)       :: 102 of 237
            Pseudo Genes (incomplete)         :: 130 of 237
            Pseudo Genes (internal stop)      :: 52 of 237
            Pseudo Genes (multiple problems)  :: 41 of 237
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..796
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267800-rid25106413.denovo.667"
                     /serotype="O 26:H11"
                     /isolate="2201S31241"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-01"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            complement(224..766)
                     /gene="fimA"
                     /locus_tag="ACHOSC_RS26685"
                     /old_locus_tag="pgaptmp_005337"
     CDS             complement(224..766)
                     /gene="fimA"
                     /locus_tag="ACHOSC_RS26685"
                     /old_locus_tag="pgaptmp_005337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308619.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbrial major subunit FimA"
                     /protein_id="HGU4551052.1"
                     /translation="MKLRFISSALAAALFAATGSYAAVVDGGTIHFEGELVNAACSVN
                     TDSADQVVTLGQYRTDIFNAVGNTSALIPFTIQLNDCDPVVAANAAVAFSGQADAIND
                     NLLAIASSTNTTTATGVGIEILDNTSAILKPDGNSFSTNQNLIPGTNVLHFSARYKGT
                     GTSASAGQANADATFIMRYE"
BASE COUNT          241 a          166 c          161 g          228 t
ORIGIN      
        1 tcatagttaa tacgccttgt gtattcctga cggcaaaaat aagaaatata aagaaatgat
       61 tgctttggtt tcttacaaca catcattccc ttttcattta ataaagcggc atgatacaaa
      121 gtgaacatga tttcgattta cattgccacc ataccgcttc agacaaccgg tacattactt
      181 tgttataaga cgtgatatat aattcaaaac aacgtggttt tgattattcg tatctcataa
      241 taaaagtcgc gtcagcattt gcttgccctg ctgatgcact tgtaccggtg cctttataac
      301 gtgcagaaaa atgaagaacg ttggtcccgg ggatcaagtt ctggttggtt gagaagctat
      361 tcccatcagg tttgagaatt gcggatgtat tatcaagtat ttcaataccg acacccgttg
      421 ctgttgttgt attggtactg gatgcaatgg ccaataaatt atcattgatt gcatcagcct
      481 gaccagaaaa tgcaacggca gcattagcgg caacaacagg atcgcagtcg ttcaactgaa
      541 tggtgaatgg aattaatgca gaggtattac caacagcatt gaaaatatcg gtacgatatt
      601 gaccgagtgt gacaacctgg tctgccgagt cagtattcac tgaacaggca gcattcacca
      661 gttcgccttc aaagtgaatt gtaccgccat ctacaacggc agcataacta cccgtagcgg
      721 cgaatagtgc ggcagccagc gcagacgaaa taaatcttaa tttcatatat attccttcaa
      781 tctcatttat cgactc
//
LOCUS       DAZKHF020000664          795 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2201S31241
            SAMN43267800-rid25106413.denovo.668, whole genome shotgun sequence.
ACCESSION   DAZKHF020000664 DAZKHF020000000
VERSION     DAZKHF020000664.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267800
            Sequence Read Archive: SRR30307263
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 795)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 795)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 795)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350581.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350581.2
            Genome Coverage       :: 151x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:21:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,652
            CDSs (total)                      :: 5,557
            Genes (coding)                    :: 5,320
            CDSs (with protein)               :: 5,320
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 237
            CDSs (without protein)            :: 237
            Pseudo Genes (ambiguous residues) :: 0 of 237
            Pseudo Genes (frameshifted)       :: 102 of 237
            Pseudo Genes (incomplete)         :: 130 of 237
            Pseudo Genes (internal stop)      :: 52 of 237
            Pseudo Genes (multiple problems)  :: 41 of 237
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..795
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267800-rid25106413.denovo.668"
                     /serotype="O 26:H11"
                     /isolate="2201S31241"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-01"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            complement(60..599)
                     /locus_tag="ACHOSC_RS26690"
                     /old_locus_tag="pgaptmp_005338"
                     /pseudo
     CDS             complement(60..599)
                     /locus_tag="ACHOSC_RS26690"
                     /old_locus_tag="pgaptmp_005338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001405821.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3987 domain-containing protein"
BASE COUNT          219 a          189 c          163 g          224 t
ORIGIN      
        1 tggacaccac tttgtctaat tcgtcagatt ctgaccagac ggttcaggct gtacgcttac
       61 actgaacgcc atttaccaca aagctgattc ttaattgccg cagtcgtggc atcattaaaa
      121 atgaatttat acctcacagg ttcttccgca ctgtttttct gaagtacttc cagttgtctg
      181 agggtttctg actcatccgc gcctttgcgg agttctttat tcagttttga caacaatgct
      241 tttttctgag cctttaaaag ctcttccttg tttttccaga cagccagttc acttttgtat
      301 ctggaatata actgcatctc ctgctggtac aggggcttca ttaataattt gtccactgcg
      361 gtttttcgtt ctccggactc ggcaagcgtc aggaaaaaaa gagagaccgg cccccgcagt
      421 gtattcagtc tgcagacatc aatcagattc tggcagacaa gtgaaacagc gcccagggca
      481 gacgctgcca caagcccaag gggagcctgc gtaatttgct gagcttcaat aacggcatca
      541 cgaatgagtg gaggcagcgc actcagggga aactcatcac aaggctgacc gaatgacata
      601 gtaacctcac tacacacaga tataaaagca ctgcaattct ggctattaat agcaaaaata
      661 gcagtcaaat caatatgagg taaaaatttt tgttgattta atggttaaat aataaccaca
      721 gtgtgttttg ctgctgaaga aagacaaaat gaatctgtgt gtcccctcct tcccttcaga
      781 gcacatccac tattc
//
LOCUS       DAZKHF020000665          793 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2201S31241
            SAMN43267800-rid25106413.denovo.669, whole genome shotgun sequence.
ACCESSION   DAZKHF020000665 DAZKHF020000000
VERSION     DAZKHF020000665.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267800
            Sequence Read Archive: SRR30307263
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 793)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 793)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 793)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350581.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350581.2
            Genome Coverage       :: 151x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:21:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,652
            CDSs (total)                      :: 5,557
            Genes (coding)                    :: 5,320
            CDSs (with protein)               :: 5,320
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 237
            CDSs (without protein)            :: 237
            Pseudo Genes (ambiguous residues) :: 0 of 237
            Pseudo Genes (frameshifted)       :: 102 of 237
            Pseudo Genes (incomplete)         :: 130 of 237
            Pseudo Genes (internal stop)      :: 52 of 237
            Pseudo Genes (multiple problems)  :: 41 of 237
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..793
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267800-rid25106413.denovo.669"
                     /serotype="O 26:H11"
                     /isolate="2201S31241"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-01"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            <1..321
                     /locus_tag="ACHOSC_RS26695"
                     /old_locus_tag="pgaptmp_005339"
     CDS             <1..321
                     /locus_tag="ACHOSC_RS26695"
                     /old_locus_tag="pgaptmp_005339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020238748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inovirus-type Gp2 protein"
                     /protein_id="HGU4551053.1"
                     /translation="REFGECKGKKHYHLMLLVNRDTWCRAGDYRAPGSLAGMIKQAWC
                     SALGVDVGCHATLVHFPAWPAVWLARNDDTGFQQVLERADYLAKEHTKVHCTGERNFG
                     CSRG"
     gene            487..>793
                     /locus_tag="ACHOSC_RS26700"
                     /old_locus_tag="pgaptmp_005340"
     CDS             487..>793
                     /locus_tag="ACHOSC_RS26700"
                     /old_locus_tag="pgaptmp_005340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004020115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative zinc ribbon protein"
                     /protein_id="HGU4551054.1"
                     /translation="MYAKSFLALDGNGRLTGARTAQAAPYAHYTCHLCGSALRYHPQY
                     DTELPWFEHTDDRLTEHGQQCPYVRPERREIQLIKRLQQFVPDALPVVRKASWHCRQC
                     "
BASE COUNT          177 a          217 c          227 g          172 t
ORIGIN      
        1 cgggagtttg gggagtgcaa aggtaaaaaa cactatcacc tgatgctgct ggtcaaccgg
       61 gatacctggt gtcgtgccgg tgattaccgc gctccgggat cactggccgg gatgattaaa
      121 caggcgtggt gcagtgccct gggagtggat gtcgggtgcc atgccacgct ggtgcatttt
      181 ccggcctggc cggcggtgtg gctggcgcgt aatgatgaca ccggctttca gcaagtgctg
      241 gaacgtgctg actatctggc gaaggagcat accaaagttc actgcaccgg tgagcgcaac
      301 tttggctgca gtcgtggctg agccagacag acgagttatc tgacttcagt gtgatgacgt
      361 gttcacggcc accacactga ttttccctta tgagactgaa ctgaaaccgc cattcacacc
      421 ggaccacgct gcccgtaccg ggacggctgt tgcctgcgtg tcgtctgacg gtaaggaaca
      481 tatactatgt acgcaaaatc ctttctcgct cttgatggca acggacgtct gacgggcgcc
      541 cgtacagcac aggccgcacc ttatgctcac tacacctgcc acttgtgtgg cagtgcactc
      601 agataccatc cgcaatacga cactgaactt ccctggtttg aacacactga cgacaggctg
      661 acagagcacg gtcaacagtg cccttatgtc aggccggagc gcagagaaat acagttgatt
      721 aaacgtctgc agcaattcgt accggatgcc ttacccgtgg tgcgtaaagc cagctggcac
      781 tgcagacaat gtc
//
LOCUS       DAZKHF020000666          791 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2201S31241
            SAMN43267800-rid25106413.denovo.670, whole genome shotgun sequence.
ACCESSION   DAZKHF020000666 DAZKHF020000000
VERSION     DAZKHF020000666.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267800
            Sequence Read Archive: SRR30307263
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 791)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 791)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 791)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350581.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350581.2
            Genome Coverage       :: 151x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:21:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,652
            CDSs (total)                      :: 5,557
            Genes (coding)                    :: 5,320
            CDSs (with protein)               :: 5,320
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 237
            CDSs (without protein)            :: 237
            Pseudo Genes (ambiguous residues) :: 0 of 237
            Pseudo Genes (frameshifted)       :: 102 of 237
            Pseudo Genes (incomplete)         :: 130 of 237
            Pseudo Genes (internal stop)      :: 52 of 237
            Pseudo Genes (multiple problems)  :: 41 of 237
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..791
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267800-rid25106413.denovo.670"
                     /serotype="O 26:H11"
                     /isolate="2201S31241"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-01"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            <1..517
                     /locus_tag="ACHOSC_RS26705"
                     /old_locus_tag="pgaptmp_005341"
     CDS             <1..517
                     /locus_tag="ACHOSC_RS26705"
                     /old_locus_tag="pgaptmp_005341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="lysozyme"
                     /protein_id="HGU4551055.1"
                     /translation="YGLSAAVLALIGAGASAPEILDQFLDEKEGNHTTAYRDGTGIWT
                     ICRGAILVDGKPVVPGMKLSKEKCDQVNAIERDKALAWVEKNIKVPLTDPQKAGIASF
                     CPYNIGPGKCFPSTFYRRINAGDRRGACEAIRWWIKDGGRDCRIRSNNCYGQVSRRDQ
                     ESALACWGIDR"
BASE COUNT          195 a          175 c          235 g          186 t
ORIGIN      
        1 atacggcctg tcggctgccg ttctggcgct gattggtgca ggggcgtctg cgcctgaaat
       61 cctcgaccag tttctggatg aaaaggaagg taaccacacc acagcatacc gtgatggtac
      121 gggtatctgg accatctgcc gcggtgccat cctggtggat ggtaaacctg tcgtcccggg
      181 catgaagttg tcgaaggaaa aatgcgacca ggttaacgcc attgagcgtg ataaggcgct
      241 ggcatgggtg gagaaaaaca tcaaagtgcc gctgaccgac ccccagaaag cggggatcgc
      301 gtcattctgt ccgtacaaca ttggtcccgg taagtgtttc ccgtcgacgt tttacagacg
      361 aattaatgct ggtgaccgca ggggagcatg cgaggcgatt cgctggtgga ttaaggacgg
      421 tggcagagac tgccgtattc gctcaaacaa ctgctacggt caggtctcac ggcgtgacca
      481 ggagagcgcg ctggcgtgct ggggaattga cagataagca gaatattttg ctgaaaaatg
      541 cggtttgctc acacgggcgg ataacacgaa atcctgcgaa ctggcaaaaa ctaagtgaat
      601 aaaagtaaaa ccccgtttgt tggccgcaag cggggttttg tgtttcctga ctccggaaaa
      661 gtcaaaggag aaagtgtgtt tgattttagc aaactgattc gggagattcg agtgatggct
      721 gaaaaattat ccacctggaa gttcatcctt atctggctgg tgtttgtgat tatggcctcc
      781 ggttatttca t
//
LOCUS       DAZKHF020000667          787 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2201S31241
            SAMN43267800-rid25106413.denovo.671, whole genome shotgun sequence.
ACCESSION   DAZKHF020000667 DAZKHF020000000
VERSION     DAZKHF020000667.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267800
            Sequence Read Archive: SRR30307263
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 787)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 787)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 787)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350581.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350581.2
            Genome Coverage       :: 151x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:21:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,652
            CDSs (total)                      :: 5,557
            Genes (coding)                    :: 5,320
            CDSs (with protein)               :: 5,320
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 237
            CDSs (without protein)            :: 237
            Pseudo Genes (ambiguous residues) :: 0 of 237
            Pseudo Genes (frameshifted)       :: 102 of 237
            Pseudo Genes (incomplete)         :: 130 of 237
            Pseudo Genes (internal stop)      :: 52 of 237
            Pseudo Genes (multiple problems)  :: 41 of 237
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..787
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267800-rid25106413.denovo.671"
                     /serotype="O 26:H11"
                     /isolate="2201S31241"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-01"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            <1..664
                     /locus_tag="ACHOSC_RS26710"
                     /old_locus_tag="pgaptmp_005342"
     CDS             <1..664
                     /locus_tag="ACHOSC_RS26710"
                     /old_locus_tag="pgaptmp_005342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4551056.1"
                     /translation="TPLFVAQGNQLFMNDVFLKRLFAASITSSGNPPTFSLTPEGKLT
                     ARNADISGAITANTGTLNNVTINENCVIRGKLSANQIEGDLVKTVGKAFPRNNSYASG
                     TVTVTVYDDQGFDRQIIIPPVLFRGTKHQNFNSPNQQSYWYSTCKLQVLKNGVEIFHE
                     PATDVSRVFSSVIDMPAGRGHVTLTFNVSSAGANNWTPTTYISDLLVVVMKKSTAGIS
                     IS"
BASE COUNT          198 a          190 c          221 g          178 t
ORIGIN      
        1 cacgccgtta tttgtggcgc aggggaatca gttgttcatg aacgatgtgt tcctgaagcg
       61 gctgtttgcg gcgagtatca cgtcatccgg caaccccccg acgttttccc tgacgccgga
      121 agggaagctg acagccagga acgcggatat cagcggagca attaccgcga ataccggcac
      181 gctcaataat gtcaccatta acgagaactg tgtcatcaga gggaaactgt ctgcaaacca
      241 gattgaaggc gacctggtga agacggtggg gaaagccttt ccccggaata acagttatgc
      301 cagcgggacg gtaaccgtca cagtttacga tgaccaggga ttcgaccggc agattatcat
      361 tcccccggtg ctgtttcgcg ggacgaaaca ccagaatttc aacagcccga atcagcagtc
      421 gtactggtat tccacctgta agctgcaggt gctgaagaac ggggttgaga ttttccatga
      481 accggcaacg gatgtcagcc gggtgttctc atcggtgata gatatgccgg cagggcgggg
      541 tcatgtcacc ctgacgttta atgtgtcgtc ggccggtgcg aacaactgga cgccgacaac
      601 gtacatcagt gatttactgg ttgtggtcat gaaaaaatcc acggcaggga tcagtatcag
      661 ctgacggttt attaacccgg acgggcacct caggaggtgc cttttttatt gactgaaaac
      721 aaagaggtaa tcatgcggca tttatacgca acgatattat tgtttactac cctgctggca
      781 ggaattg
//
LOCUS       DAZKHF020000668          780 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2201S31241
            SAMN43267800-rid25106413.denovo.672, whole genome shotgun sequence.
ACCESSION   DAZKHF020000668 DAZKHF020000000
VERSION     DAZKHF020000668.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267800
            Sequence Read Archive: SRR30307263
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 780)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 780)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 780)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350581.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350581.2
            Genome Coverage       :: 151x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:21:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,652
            CDSs (total)                      :: 5,557
            Genes (coding)                    :: 5,320
            CDSs (with protein)               :: 5,320
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 237
            CDSs (without protein)            :: 237
            Pseudo Genes (ambiguous residues) :: 0 of 237
            Pseudo Genes (frameshifted)       :: 102 of 237
            Pseudo Genes (incomplete)         :: 130 of 237
            Pseudo Genes (internal stop)      :: 52 of 237
            Pseudo Genes (multiple problems)  :: 41 of 237
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..780
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267800-rid25106413.denovo.672"
                     /serotype="O 26:H11"
                     /isolate="2201S31241"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-01"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            <1..>780
                     /locus_tag="ACHOSC_RS26715"
                     /old_locus_tag="pgaptmp_005343"
     CDS             <1..>780
                     /locus_tag="ACHOSC_RS26715"
                     /old_locus_tag="pgaptmp_005343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="terminase gpA endonuclease subunit"
                     /protein_id="HGU4551057.1"
                     /translation="LAPWYGKKHRDNTLTMKRFSNGRGFWCLGGKAAKNYREKSVDVA
                     GYDELAAFDEDIEQEGSPTFLGDKRIEGSVWPKSIRGSTPKVRGTCQIERAASESPHF
                     MRFHVACPHCGEEQYLKFGDKETPFGLKWTPDDPSSVFYLCEHNACVIRQQELDFTDA
                     RYICEKTGIWTRDGILWFSSSGEEIEPPDSVTFHIWTAYSPFTTWVQIVKDWMKTKGD
                     TGKRKTFVNTTLGETWEAKIGERPDAEVMAERKEYYSAPVP"
BASE COUNT          185 a          190 c          231 g          174 t
ORIGIN      
        1 gctggctccg tggtatggca aaaagcaccg ggataacacg ctcactatga agcgtttttc
       61 caatggtcgt ggcttctggt gcctgggcgg taaagcggca aaaaactacc gtgaaaagtc
      121 ggtggatgtg gcgggttatg atgaactggc tgcctttgat gaggatattg aacaggaagg
      181 ctctccgacg ttcctgggcg ataagcgtat tgaaggctcg gtctggccaa agtccatccg
      241 tggctccacg cccaaagtga gaggcacctg ccagattgag cgtgcagcca gtgaatcccc
      301 gcattttatg cgttttcatg ttgcctgccc gcactgcggg gaggagcagt atcttaaatt
      361 tggcgacaaa gagacgccgt ttggcctcaa atggacgccg gatgacccct ccagcgtgtt
      421 ttatctctgc gagcataatg cctgcgtcat ccgccagcag gagctggact ttactgatgc
      481 ccgttatatc tgcgaaaaga ccgggatctg gacccgtgat ggcattctct ggttttcgtc
      541 atccggtgaa gagattgaac cgcctgacag tgtgaccttt cacatctgga cggcgtacag
      601 cccgttcacc acctgggtgc agattgtcaa agactggatg aagacgaaag gggatacggg
      661 aaaacgtaaa accttcgtga acaccacgct cggtgagacg tgggaagcga aaatcggcga
      721 acgtccggat gctgaagtga tggcagagcg gaaagagtat tattcagcgc ccgttcctga
//
LOCUS       DAZKHF020000669          779 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2201S31241
            SAMN43267800-rid25106413.denovo.673, whole genome shotgun sequence.
ACCESSION   DAZKHF020000669 DAZKHF020000000
VERSION     DAZKHF020000669.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267800
            Sequence Read Archive: SRR30307263
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 779)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 779)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 779)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350581.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350581.2
            Genome Coverage       :: 151x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:21:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,652
            CDSs (total)                      :: 5,557
            Genes (coding)                    :: 5,320
            CDSs (with protein)               :: 5,320
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 237
            CDSs (without protein)            :: 237
            Pseudo Genes (ambiguous residues) :: 0 of 237
            Pseudo Genes (frameshifted)       :: 102 of 237
            Pseudo Genes (incomplete)         :: 130 of 237
            Pseudo Genes (internal stop)      :: 52 of 237
            Pseudo Genes (multiple problems)  :: 41 of 237
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..779
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267800-rid25106413.denovo.673"
                     /serotype="O 26:H11"
                     /isolate="2201S31241"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-01"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            <1..478
                     /locus_tag="ACHOSC_RS26720"
                     /old_locus_tag="pgaptmp_005344"
     CDS             <1..478
                     /locus_tag="ACHOSC_RS26720"
                     /old_locus_tag="pgaptmp_005344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310263.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="tail assembly protein"
                     /protein_id="HGU4551058.1"
                     /translation="MAPEAVYARLHEQLGEGTVIHIVPRLAGAGKGGLQIVLGAAAIV
                     GSFFTAGATMALWGSALAAGGFSATTMLFSLGASMILGGVAQMLAPKAKTPEYKSTDN
                     GKQNTYFSSLDNMIAQGNPMPVPYGEMLVGSRRISQDISTRDEGGGGKVVVIGRQG"
BASE COUNT          190 a          180 c          256 g          153 t
ORIGIN      
        1 catggcaccg gaggcggtgt atgcccgcct tcacgaacaa ctgggtgagg gaacggtcat
       61 ccatattgtg ccgcgactgg ccggtgcagg gaaaggcgga ctgcagattg tgctgggggc
      121 ggcagccatc gtgggctctt tcttcaccgc cggcgcaacg atggcgttat ggggttcagc
      181 cctggcagcc ggcggttttt ctgccaccac gatgctgttt tcactggggg ccagcatgat
      241 actgggcggt gtggcccaga tgctggcccc gaaggcaaag acgccggagt acaaaagtac
      301 ggataacggt aaacagaaca cgtatttttc gtcactggac aacatgattg cccaggggaa
      361 cccgatgccg gtgccttacg gtgaaatgct ggttggctcc cggcgaatct cccaggacat
      421 cagcacccgt gatgaaggtg gtggcgggaa ggtggtggtt atcgggcggc aggggtaaag
      481 cataaaaaaa tcccgcagtg tatggaggct gcgggaacag aaaatgaaga ttaaccacag
      541 ggggttttgt ttttattggc ccgaaaaaac tgtaacgccc gggaatgata tctgccacgg
      601 ggcgtacaga aaatgtgaag aaattcagaa attttattcc gtcatgacac aggcaccctc
      661 cggggtgcct gtcgtttttg gggcataaac agattcagac atcagacagg agagggggac
      721 agagtgggta aaggtggcgg cagggcgcac acgccggtag aggcaaagga caatcttaa
//
LOCUS       DAZKHF020000670          779 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2201S31241
            SAMN43267800-rid25106413.denovo.674, whole genome shotgun sequence.
ACCESSION   DAZKHF020000670 DAZKHF020000000
VERSION     DAZKHF020000670.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267800
            Sequence Read Archive: SRR30307263
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 779)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 779)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 779)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350581.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350581.2
            Genome Coverage       :: 151x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:21:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,652
            CDSs (total)                      :: 5,557
            Genes (coding)                    :: 5,320
            CDSs (with protein)               :: 5,320
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 237
            CDSs (without protein)            :: 237
            Pseudo Genes (ambiguous residues) :: 0 of 237
            Pseudo Genes (frameshifted)       :: 102 of 237
            Pseudo Genes (incomplete)         :: 130 of 237
            Pseudo Genes (internal stop)      :: 52 of 237
            Pseudo Genes (multiple problems)  :: 41 of 237
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..779
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267800-rid25106413.denovo.674"
                     /serotype="O 26:H11"
                     /isolate="2201S31241"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-01"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            37..408
                     /locus_tag="ACHOSC_RS26725"
                     /old_locus_tag="pgaptmp_005345"
     CDS             37..408
                     /locus_tag="ACHOSC_RS26725"
                     /old_locus_tag="pgaptmp_005345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RusA family crossover junction
                     endodeoxyribonuclease"
                     /protein_id="HGU4551059.1"
                     /translation="MRIEFVLPYPPTVNTYWRRRGSTYFVSKAGERYRRAVALIVRQQ
                     RLKLSLSGRLAIKIIAEPPDKRRRDLDNILKAPLDALTHAEVLIDDEQFDEINIVRGQ
                     PVPGGRLGVKIYEIRGGNDGA"
     gene            398..769
                     /locus_tag="ACHOSC_RS26730"
                     /old_locus_tag="pgaptmp_005346"
     CDS             398..769
                     /locus_tag="ACHOSC_RS26730"
                     /old_locus_tag="pgaptmp_005346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000090266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0060567 - negative regulation of
                     termination of DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="antiterminator Q family protein"
                     /protein_id="HGU4551060.1"
                     /translation="MARDIQMVLERWGAWAANNHEDVTWPSIAAGFKGLIPTKVKSRP
                     QCSDDDAMIICGCMARLNKNNQYLHDLLVDYYVGGMTFMALARKHRCSDGLIGKRLYK
                     AEGIIEGMLMALNVRLDMDMR"
BASE COUNT          201 a          144 c          226 g          208 t
ORIGIN      
        1 tttgcaaccg gcgtgctcgg gtaaaagagg ttactgatgc gtatagagtt tgttttgcct
       61 tacccgccga cggtgaacac ctactggcga cgtcgtggca gcacatattt tgtatcaaaa
      121 gccggtgagc gttatcgccg ggctgtggcg cttattgttc gccagcagcg gctgaaatta
      181 agcctgtccg gacggctggc aataaaaatt attgcagaac caccggataa gcgccgccgt
      241 gacctggaca acattctgaa agcaccgctg gatgcgctga cgcatgcgga agtgctcatt
      301 gatgacgagc agtttgatga aatcaatatt gtgcgcggtc agcctgtgcc aggtggacgg
      361 ctgggcgtga agatttacga aatcagaggt ggtaacgatg gcgcgtgata tccagatggt
      421 tcttgagcga tggggggcat gggcagcaaa taatcatgaa gatgtaacat ggccctcgat
      481 agctgctggt tttaaaggat taatcccgac taaagtgaaa tcacgtcctc agtgttctga
      541 tgatgacgcc atgataattt gtggttgtat ggcacgatta aacaagaata atcagtattt
      601 gcacgatttg ttggtggatt attacgtagg tggaatgaca tttatggctc ttgcacgtaa
      661 gcatagatgt tctgatgggc ttattggtaa aaggctttat aaagcggaag gtattattga
      721 aggaatgctt atggctctga atgtccggtt agatatggat atgcggtagg gatatatag
//
LOCUS       DAZKHF020000671          777 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2201S31241
            SAMN43267800-rid25106413.denovo.675, whole genome shotgun sequence.
ACCESSION   DAZKHF020000671 DAZKHF020000000
VERSION     DAZKHF020000671.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267800
            Sequence Read Archive: SRR30307263
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 777)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 777)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 777)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350581.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350581.2
            Genome Coverage       :: 151x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:21:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,652
            CDSs (total)                      :: 5,557
            Genes (coding)                    :: 5,320
            CDSs (with protein)               :: 5,320
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 237
            CDSs (without protein)            :: 237
            Pseudo Genes (ambiguous residues) :: 0 of 237
            Pseudo Genes (frameshifted)       :: 102 of 237
            Pseudo Genes (incomplete)         :: 130 of 237
            Pseudo Genes (internal stop)      :: 52 of 237
            Pseudo Genes (multiple problems)  :: 41 of 237
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..777
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267800-rid25106413.denovo.675"
                     /serotype="O 26:H11"
                     /isolate="2201S31241"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-01"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            219..581
                     /locus_tag="ACHOSC_RS26735"
                     /old_locus_tag="pgaptmp_005347"
     CDS             219..581
                     /locus_tag="ACHOSC_RS26735"
                     /old_locus_tag="pgaptmp_005347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000193240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4551061.1"
                     /translation="MTQNVLPINKSLHDRAVDEFNRLHGTMIGEISAMLKTAKVAPLV
                     DLRKKDPTFSNVVAELRTFRDVCNALLPYFRVDKTSEIAVIDKLLILANDLAQAIDAD
                     DPDALCAAIAALDVEPYI"
     gene            597..725
                     /locus_tag="ACHOSC_RS26740"
                     /old_locus_tag="pgaptmp_005348"
     CDS             597..725
                     /locus_tag="ACHOSC_RS26740"
                     /old_locus_tag="pgaptmp_005348"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4551062.1"
                     /translation="MTKAFDYTAVSKLLAEMRGCVERVQNLRRDFEAHINHSQKAA"
BASE COUNT          238 a          151 c          165 g          223 t
ORIGIN      
        1 atgtgataga gatcattatt caattcgatt gagaaaaatc atcataaggt ttatatccat
       61 ttattagatt ttatgtgtat aaatagttaa aaatgcacga tgtagagata tttgtgctta
      121 acatcaatta atcattgcta aatcgtgctt attgttcaga aatcgcccaa gtcatattga
      181 aaatctgttg gcgaacacct atataaggag tatagttagt gacccaaaac gtattgccaa
      241 tcaacaagag cttgcatgac cgagctgttg atgaatttaa tcgtctgcat ggaacaatga
      301 ttggcgagat tagtgcaatg ctgaaaacag ctaaagtagc gccattggtg gatcttcgta
      361 agaaagatcc taccttttcg aacgttgtcg cagaattacg gacattcaga gacgtatgta
      421 atgctctgct cccatacttt cgtgtagata agaccagcga aatcgctgtc atcgataagt
      481 tacttatact ggctaatgac ctggctcaag ccatcgatgc tgacgatcct gacgcattgt
      541 gcgcagcaat agcagccctt gatgttgagc cttacattta aacgagggga aaaaaaatga
      601 ctaaagcgtt tgactatact gccgttagta agcttttggc tgaaatgcgc ggatgtgtag
      661 agcgcgttca aaatctacgc cgcgactttg aagctcacat caatcattca caaaaagcgg
      721 cttgatactt aagttagatg acagaacccg gcctcagcgc cgggttttct ttgcctc
//
LOCUS       DAZKHF020000672          776 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2201S31241
            SAMN43267800-rid25106413.denovo.676, whole genome shotgun sequence.
ACCESSION   DAZKHF020000672 DAZKHF020000000
VERSION     DAZKHF020000672.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267800
            Sequence Read Archive: SRR30307263
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 776)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 776)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 776)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350581.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350581.2
            Genome Coverage       :: 151x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:21:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,652
            CDSs (total)                      :: 5,557
            Genes (coding)                    :: 5,320
            CDSs (with protein)               :: 5,320
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 237
            CDSs (without protein)            :: 237
            Pseudo Genes (ambiguous residues) :: 0 of 237
            Pseudo Genes (frameshifted)       :: 102 of 237
            Pseudo Genes (incomplete)         :: 130 of 237
            Pseudo Genes (internal stop)      :: 52 of 237
            Pseudo Genes (multiple problems)  :: 41 of 237
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..776
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267800-rid25106413.denovo.676"
                     /serotype="O 26:H11"
                     /isolate="2201S31241"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-01"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            <1..>776
                     /locus_tag="ACHOSC_RS26745"
                     /old_locus_tag="pgaptmp_005349"
     CDS             <1..>776
                     /locus_tag="ACHOSC_RS26745"
                     /old_locus_tag="pgaptmp_005349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313311.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF2686 family protein"
                     /protein_id="HGU4551063.1"
                     /translation="SNTLQSQIITDNHFLHHPKVDSELTRKYERARLDTENIYLLPLA
                     RGNNHNYDGKSVVEIRKLDISKESWPFNYVTEACRESDGITTTGRMLYRNLKITSALD
                     EIYGGICKKAHAATELAEGLRLNLFMKSPFDPVENYTVHEITLGPGCNVPGYAGTTIG
                     YISTLPVSQAKRWTNEQPRIDIYIDQIITVSGVANSSGFALAALLNANIEMGNDPIIG
                     IEAYPGTAEIHAKMGYKVIPGDEDAPLKRMTLQPSSLPEL"
BASE COUNT          253 a          163 c          155 g          205 t
ORIGIN      
        1 aagcaatacc cttcagagcc aaattattac agataatcac ttccttcatc atccaaaagt
       61 cgacagtgag ttaacacgta aatatgaacg tgcacgcctg gatacggaaa atatctatct
      121 tctgcctctt gctcgtggca ataaccataa ttacgatgga aaaagcgttg tcgaaattag
      181 aaaacttgat attagtaaag agtcctggcc atttaattat gtgacagaag catgcagaga
      241 atccgatggt attacaacaa caggacgtat gctctatcga aacttgaaaa tcacctcagc
      301 tttagacgaa atatatggtg gcatatgtaa aaaggcgcat gctgctacag aattagcaga
      361 aggtttacgg ctaaatttat ttatgaagag ccctttcgat cctgttgaga actatacagt
      421 tcatgaaata acattaggcc ctggttgcaa tgttccaggt tacgccggaa ccacgatagg
      481 atatatttca acccttccag tttctcaggc aaaaagatgg accaatgagc agccaagaat
      541 tgatatctat attgatcaga taataactgt ctcaggggta gcaaattctt ccggattcgc
      601 ccttgccgca ttacttaatg ctaatattga gatgggaaat gacccaatca ttggtataga
      661 agcctatcct ggtaccgcgg agatccatgc aaaaatggga tataaagtta tccccggtga
      721 tgaagatgcg ccactcaaac gcatgactct gcaaccgtca tctcttccag aactgt
//
LOCUS       DAZKHF020000673          774 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2201S31241
            SAMN43267800-rid25106413.denovo.677, whole genome shotgun sequence.
ACCESSION   DAZKHF020000673 DAZKHF020000000
VERSION     DAZKHF020000673.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267800
            Sequence Read Archive: SRR30307263
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 774)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 774)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 774)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350581.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350581.2
            Genome Coverage       :: 151x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:21:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,652
            CDSs (total)                      :: 5,557
            Genes (coding)                    :: 5,320
            CDSs (with protein)               :: 5,320
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 237
            CDSs (without protein)            :: 237
            Pseudo Genes (ambiguous residues) :: 0 of 237
            Pseudo Genes (frameshifted)       :: 102 of 237
            Pseudo Genes (incomplete)         :: 130 of 237
            Pseudo Genes (internal stop)      :: 52 of 237
            Pseudo Genes (multiple problems)  :: 41 of 237
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..774
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267800-rid25106413.denovo.677"
                     /serotype="O 26:H11"
                     /isolate="2201S31241"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-01"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            19..>774
                     /locus_tag="ACHOSC_RS26750"
                     /old_locus_tag="pgaptmp_005350"
     CDS             19..>774
                     /locus_tag="ACHOSC_RS26750"
                     /old_locus_tag="pgaptmp_005350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4551064.1"
                     /translation="MNSKKLCCICVLFSLLAGCASESSIDEKKKKAQVTQSNINKNTP
                     QQLTDKDLFGNETTLAVSEEDIQAALDGDEFRVPLNSPVILVQSGNRAPETIMQEEMR
                     KYYTVSTFSGIPDRQKPLTCNKNKDKNENEDVASAENMNWMQALRFVAAKGHQKAIIV
                     FQDTLQTGKYDSALKSTVWSDYKNEKLTDAISLRYLVRFTLVDVATGEWATWSPVNYE
                     YKVLPPLPDKNEASTTDMTEQQIMQLKQKTYKAM"
BASE COUNT          247 a          163 c          176 g          188 t
ORIGIN      
        1 gcgagggagt gtccaattat gaattcaaaa aagctttgtt gcatatgtgt gttattctcg
       61 ctgcttgcag gatgtgcctc tgaatcttct attgatgaaa agaagaaaaa agctcaagtc
      121 acacaaagta atattaataa aaacactccc cagcaactga cagacaaaga tttattcggt
      181 aatgaaacca ctctggccgt atccgaagag gatattcaag ctgcgcttga tggagatgag
      241 ttccgcgttc cccttaattc tcctgtaata cttgttcaat ccggcaaccg cgcaccggaa
      301 accattatgc aggaggagat gcgaaaatat tatactgttt ccacattctc tggtatcccg
      361 gacaggcaaa agcctctgac ttgtaacaaa aacaaagata aaaatgagaa cgaggatgtt
      421 gccagtgctg agaatatgaa ctggatgcag gcactgcgtt ttgtggctgc aaaagggcat
      481 cagaaagcga ttattgtttt ccaggatacg ttgcagacag gaaaatatga ctctgcgctg
      541 aaatcaacag tatggtccga ctataaaaat gaaaaactca ccgacgctat ctccctgcgc
      601 tacctggtac gtttcacgct ggtggatgtg gcaacaggtg agtgggctac ctggtcgccg
      661 gtgaattatg aatataaagt gcttccacca ttgcccgaca agaatgaagc cagtacgact
      721 gatatgacag agcagcaaat catgcaactt aagcaaaaga cctataaagc gatg
//
LOCUS       DAZKHF020000674          773 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2201S31241
            SAMN43267800-rid25106413.denovo.678, whole genome shotgun sequence.
ACCESSION   DAZKHF020000674 DAZKHF020000000
VERSION     DAZKHF020000674.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267800
            Sequence Read Archive: SRR30307263
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 773)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 773)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 773)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350581.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350581.2
            Genome Coverage       :: 151x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:21:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,652
            CDSs (total)                      :: 5,557
            Genes (coding)                    :: 5,320
            CDSs (with protein)               :: 5,320
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 237
            CDSs (without protein)            :: 237
            Pseudo Genes (ambiguous residues) :: 0 of 237
            Pseudo Genes (frameshifted)       :: 102 of 237
            Pseudo Genes (incomplete)         :: 130 of 237
            Pseudo Genes (internal stop)      :: 52 of 237
            Pseudo Genes (multiple problems)  :: 41 of 237
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..773
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267800-rid25106413.denovo.678"
                     /serotype="O 26:H11"
                     /isolate="2201S31241"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-01"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            complement(<1..563)
                     /gene="ehxB"
                     /locus_tag="ACHOSC_RS26755"
                     /old_locus_tag="pgaptmp_005351"
                     /gene_synonym="hlyB"
     CDS             complement(<1..563)
                     /gene="ehxB"
                     /locus_tag="ACHOSC_RS26755"
                     /old_locus_tag="pgaptmp_005351"
                     /gene_synonym="hlyB"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enterohemolysin T1SS ABC transporter
                     permease/ATPase EhxB"
                     /protein_id="HGU4551065.1"
                     /translation="MMSKCSSHNSLYALILLAQYHNITVNAETIRHQYNTHTQDFGVT
                     EWLLAAKSIGLKAKYVEKHFSRLSIISLPALIWRDDGKHYILSRVTKDSSRYLVYDPE
                     QHQSLTFSRDEFEKLYQGKVILVTSRATVVGELAKFDFSWFIPSVVKYRRILLEVLTV
                     SAFIQFLALITPLFFQVVMDKVLVHRG"
     gene            complement(613..>773)
                     /locus_tag="ACHOSC_RS26760"
                     /old_locus_tag="pgaptmp_005352"
     CDS             complement(613..>773)
                     /locus_tag="ACHOSC_RS26760"
                     /old_locus_tag="pgaptmp_005352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4551066.1"
                     /translation="KNMVNITSVANDINKIISSVSGFDSGDERLASLYNLSLHQNNTH
                     STTLTTTV"
BASE COUNT          258 a          151 c          126 g          238 t
ORIGIN      
        1 aacccccggt gaactaaaac cttatccatt acaacctgaa aaaaaagagg tgttattaac
       61 gcaagaaact gaataaaagc agaaacagtt aacacctcaa gtaaaatcct cctgtatttc
      121 acaacagagg ggataaacca agaaaaatca aatttagcta actctccgac tactgttgct
      181 cttgacgtaa ccagaatgac ttttccctga tacagttttt caaactcatc ccgactaaaa
      241 gttagtgact gatgttgttc tggatcataa acaagatagc gtgatgaatc tttagtaaca
      301 cgagacaata tataatgctt accgtcatcc cgccatatca acgcaggtaa agaaattatt
      361 gacaatctgg aaaaatgttt ttctacatat tttgctttta agccaatgga tttcgctgcc
      421 agtaaccatt cagtcacccc aaaatcttgt gtgtgggtat tatactgatg ccttatagtt
      481 tcagcattga cagttatatt atgatattgt gcaagcaata tcagtgcata cagactatta
      541 tgagaactac atttactcat catataacga tgaccattcc tcctggaatg gccatcacct
      601 cctcttttag tctcagacag ttgtcgttaa agttgttgag tgtgtgttgt tttgatgtaa
      661 ggataaatta tataaagatg ctaatcgttc atcacctgaa tcgaaccctg aaactgaaga
      721 aataatctta ttaatatcat tggcaacact ggtgatatta accatgtttt ttt
//
LOCUS       DAZKHF020000675          766 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2201S31241
            SAMN43267800-rid25106413.denovo.679, whole genome shotgun sequence.
ACCESSION   DAZKHF020000675 DAZKHF020000000
VERSION     DAZKHF020000675.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267800
            Sequence Read Archive: SRR30307263
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 766)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 766)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 766)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350581.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350581.2
            Genome Coverage       :: 151x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:21:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,652
            CDSs (total)                      :: 5,557
            Genes (coding)                    :: 5,320
            CDSs (with protein)               :: 5,320
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 237
            CDSs (without protein)            :: 237
            Pseudo Genes (ambiguous residues) :: 0 of 237
            Pseudo Genes (frameshifted)       :: 102 of 237
            Pseudo Genes (incomplete)         :: 130 of 237
            Pseudo Genes (internal stop)      :: 52 of 237
            Pseudo Genes (multiple problems)  :: 41 of 237
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..766
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267800-rid25106413.denovo.679"
                     /serotype="O 26:H11"
                     /isolate="2201S31241"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-01"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            complement(<1..438)
                     /gene="yhaB"
                     /locus_tag="ACHOSC_RS26765"
                     /old_locus_tag="pgaptmp_005353"
     CDS             complement(<1..438)
                     /gene="yhaB"
                     /locus_tag="ACHOSC_RS26765"
                     /old_locus_tag="pgaptmp_005353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417590.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YhaB"
                     /protein_id="HGU4551067.1"
                     /translation="MKGFPIAHIFHPSIPPMHAVVNNHNRNIDYWTVKRKFAEIVSTN
                     DVNKIYSISNELRRVLSAITALNFYQGDVPSVMIRIQPENMSPFIIDISTGEHDDYII
                     QTLDVGTFAPFGEQCTCSAVNKKELECIKETISKYCAKFTRKEA"
     gene            complement(694..>766)
                     /locus_tag="ACHOSC_RS26770"
                     /old_locus_tag="pgaptmp_005354"
     CDS             complement(694..>766)
                     /locus_tag="ACHOSC_RS26770"
                     /old_locus_tag="pgaptmp_005354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417589.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DNA-binding transcriptional activator TdcR"
                     /protein_id="HGU4551068.1"
                     /translation="EQLINVNFFSSDRTSFCECNRFP"
BASE COUNT          220 a          151 c          146 g          249 t
ORIGIN      
        1 ggcttctttt cgggtgaatt ttgcacaata cttagaaatc gtctccttaa tacattccag
       61 ctctttttta ttgacggctg agcaagtaca ttgttcgcca aaaggtgcaa aagtgcctac
      121 atctaatgtt tgtatgatat aatcatcatg ttcccctgta gaaatatcta tgatgaatgg
      181 actcatattt tccggttgga ttcggatcat tacagaagga acatcgcctt gatagaaatt
      241 caatgcagta attgcagata atactctccg cagttcatta cttatactgt aaattttatt
      301 aacgtcattg gtggagacaa tttctgcaaa ctttcttttt accgtccaat aatcaatatt
      361 tctattgtga ttgttaacca ctgcgtgcat tggcgggatt gaagggtgaa aaatatgtgc
      421 aattgggaag cctttcattg atgcctgcct tgtcgtcatg acaaacaata atagatgctt
      481 attttcctcg cagggttctt tcgcactatc aaaaaacgct catttacccg gcgttacagt
      541 aaacgaagca acattctgtg gcctacgacg atcaaaagct ctgtgaaaat ttcccttgtt
      601 gtctttgtct gaccttatcg cggcgaccag caattattcg ttttgtgatc ctcaacacaa
      661 aatgctattc gtcgcctatg agtggagaag aatttatgga aaacgattgc attcacaaaa
      721 tgacgtcctg tcagaagaga aaaagttgac gttaatcaat tgttcg
//
LOCUS       DAZKHF020000676          761 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2201S31241
            SAMN43267800-rid25106413.denovo.680, whole genome shotgun sequence.
ACCESSION   DAZKHF020000676 DAZKHF020000000
VERSION     DAZKHF020000676.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267800
            Sequence Read Archive: SRR30307263
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 761)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 761)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 761)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350581.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350581.2
            Genome Coverage       :: 151x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:21:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,652
            CDSs (total)                      :: 5,557
            Genes (coding)                    :: 5,320
            CDSs (with protein)               :: 5,320
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 237
            CDSs (without protein)            :: 237
            Pseudo Genes (ambiguous residues) :: 0 of 237
            Pseudo Genes (frameshifted)       :: 102 of 237
            Pseudo Genes (incomplete)         :: 130 of 237
            Pseudo Genes (internal stop)      :: 52 of 237
            Pseudo Genes (multiple problems)  :: 41 of 237
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..761
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267800-rid25106413.denovo.680"
                     /serotype="O 26:H11"
                     /isolate="2201S31241"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-01"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            complement(<1..476)
                     /locus_tag="ACHOSC_RS26775"
                     /old_locus_tag="pgaptmp_005355"
     CDS             complement(<1..476)
                     /locus_tag="ACHOSC_RS26775"
                     /old_locus_tag="pgaptmp_005355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4551069.1"
                     /translation="MRVLLRPVLVPELGLVIVKPGRESMPVFHNTRVLVEPEPKSMRN
                     LPSGVVPAVRQPLAEDKSLLPFFSDERVIRAAGGAGALSDWLLRHVKSCQWPHGDYHH
                     SETVIHRYGTGAMVLCWHCDNQLRDQTSESLEHLAQQNLAAWMIDVIRHAMNGSQER"
     gene            complement(478..756)
                     /locus_tag="ACHOSC_RS26780"
                     /old_locus_tag="pgaptmp_005356"
     CDS             complement(478..756)
                     /locus_tag="ACHOSC_RS26780"
                     /old_locus_tag="pgaptmp_005356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4551070.1"
                     /translation="MAHDTKFYNSDNSAAPASRHGRRSRTFKSDWFQHPPCTEEQAEW
                     LIQCYRRHGYEIKKALSLDYRHWIISVRLPYSERPPRPSRTFQQRIWR"
BASE COUNT          164 a          200 c          225 g          172 t
ORIGIN      
        1 cgctcctgcg agccattcat tgcatggcgt atgacgtcaa tcatccaggc tgccaggttt
       61 tgttgagcca gatgttccag tgattcggag gtctggtcgc gcagctggtt gtcgcagtgc
      121 cagcacaaca ccattgcgcc ggtaccataa cggtgaatga cggtttcact gtggtgataa
      181 tcaccgtgtg gccactggca ggatttaaca tggcgcagta accagtcaga caatgcgcca
      241 gcgccaccag cagcacgaat cactcgttcg tcgctgaaaa atggcagtaa tgatttatcc
      301 tccgccagcg gctggcgaac ggcaggaacg accccggacg gcagattacg catgcttttc
      361 ggttccggct ccaccagtac ccgggtattg tggaataccg gcatggattc acggcccggc
      421 ttaacgatca ccagcccgag ttccggtacc agaacaggtc gaagtaatac ccgcacgtta
      481 cctccagatg cgttgctgga atgtgcggga cggacgcggt ggtcgttcgg agtaaggaag
      541 cctgacggag attatccagt gacgataatc gaggctgagg gctttcttaa tctcgtatcc
      601 gtgtctgcgg tagcactgaa ttagccactc ggcctgttct tcagtgcatg gggggtgctg
      661 gaaccagtca gatttgaaag tgcgggaacg ccgcccgtgc ctgctggcag gggcggcaga
      721 gttatccgaa ttgtaaaatt tggtatcgtg cgccatctgt t
//
LOCUS       DAZKHF020000677          757 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2201S31241
            SAMN43267800-rid25106413.denovo.681, whole genome shotgun sequence.
ACCESSION   DAZKHF020000677 DAZKHF020000000
VERSION     DAZKHF020000677.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267800
            Sequence Read Archive: SRR30307263
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 757)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 757)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 757)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350581.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350581.2
            Genome Coverage       :: 151x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:21:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,652
            CDSs (total)                      :: 5,557
            Genes (coding)                    :: 5,320
            CDSs (with protein)               :: 5,320
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 237
            CDSs (without protein)            :: 237
            Pseudo Genes (ambiguous residues) :: 0 of 237
            Pseudo Genes (frameshifted)       :: 102 of 237
            Pseudo Genes (incomplete)         :: 130 of 237
            Pseudo Genes (internal stop)      :: 52 of 237
            Pseudo Genes (multiple problems)  :: 41 of 237
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..757
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267800-rid25106413.denovo.681"
                     /serotype="O 26:H11"
                     /isolate="2201S31241"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-01"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            <1..>757
                     /locus_tag="ACHOSC_RS26785"
                     /old_locus_tag="pgaptmp_005357"
     CDS             <1..>757
                     /locus_tag="ACHOSC_RS26785"
                     /old_locus_tag="pgaptmp_005357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="major capsid protein"
                     /protein_id="HGU4551071.1"
                     /translation="SRGGSTSEFTPGYVKPKHEVNPQMTLRRLPDEDPQNLADPAYRR
                     RRIIMQNMRDEELAIAQVEEMQAVSAVLKGKYTMTGEAFDPVEVDMGRSAANNITQSG
                     GTEWSKRDKSTYDPTDDIEAYALNASGVVNIIVFDPKGWALFRSFKAVRDKLDTRRGS
                     HSELETAVKDLGKAVSYKGMYGDVAIVVYSGQYVENGVKKNFLPDNTMVLGNTQARGL
                     RTYGCIQDADAQREGINASARYPKNWVTTGDPAR"
BASE COUNT          178 a          204 c          232 g          143 t
ORIGIN      
        1 tcccgtggcg gctccacctc tgaatttacg ccgggatatg tcaagccgaa gcatgaggtg
       61 aatccgcaga tgaccctgcg tcgcctgccg gatgaagatc cgcagaatct ggcggacccg
      121 gcttaccgcc gccgtcgcat catcatgcag aacatgcgtg acgaagagct ggccattgcc
      181 caggtcgaag agatgcaggc agtttctgcc gtgcttaagg gcaaatacac catgaccggt
      241 gaagcctttg atccggttga ggtggatatg ggccgcagtg cggcaaacaa catcacacag
      301 tccggtggca cggagtggag caagcgtgac aagtccacgt atgacccgac cgacgatatc
      361 gaagcctacg cgctgaacgc cagcggcgtg gtgaatatca tcgtgtttga tccgaaagga
      421 tgggcgctgt tccgttcctt caaagccgtc agggataagc tggatacccg tcgcggctct
      481 cattccgagc tggagacagc ggtaaaagac ctgggcaaag cggtgtctta taagggaatg
      541 tatggcgatg tggccatcgt cgtgtattcc ggacagtacg tggaaaacgg cgtcaaaaag
      601 aacttcctgc cggacaacac gatggtgctg gggaacactc aggcacgcgg tctgcgtacc
      661 tatggctgta ttcaggatgc ggacgcacag cgcgaaggca ttaacgcctc tgcccgttac
      721 ccgaaaaact gggtgactac cggcgatccg gcgcgag
//
LOCUS       DAZKHF020000678          756 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2201S31241
            SAMN43267800-rid25106413.denovo.682, whole genome shotgun sequence.
ACCESSION   DAZKHF020000678 DAZKHF020000000
VERSION     DAZKHF020000678.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267800
            Sequence Read Archive: SRR30307263
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 756)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 756)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 756)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350581.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350581.2
            Genome Coverage       :: 151x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:21:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,652
            CDSs (total)                      :: 5,557
            Genes (coding)                    :: 5,320
            CDSs (with protein)               :: 5,320
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 237
            CDSs (without protein)            :: 237
            Pseudo Genes (ambiguous residues) :: 0 of 237
            Pseudo Genes (frameshifted)       :: 102 of 237
            Pseudo Genes (incomplete)         :: 130 of 237
            Pseudo Genes (internal stop)      :: 52 of 237
            Pseudo Genes (multiple problems)  :: 41 of 237
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..756
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267800-rid25106413.denovo.682"
                     /serotype="O 26:H11"
                     /isolate="2201S31241"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-01"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            157..723
                     /gene="slp"
                     /locus_tag="ACHOSC_RS26790"
                     /old_locus_tag="pgaptmp_005358"
     CDS             157..723
                     /gene="slp"
                     /locus_tag="ACHOSC_RS26790"
                     /old_locus_tag="pgaptmp_005358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312404.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipoprotein Slp"
                     /protein_id="HGU4551072.1"
                     /translation="MNMTKGALILSLSFLLAACSSIPQNIKGNNQPDIQKSFVAVHNQ
                     PGLYVGQQARFGGKVINVINGKTDTLLEIAVLPLDSYAKPDIEANYQGRLLARQSGFL
                     DPVNYRNHFVTILGTIQGEQPGFINKVPYNFLEVNMQGIQVWHLREVVNTTYNLWDYG
                     YGAFWPEPGWGAPYYTNAVSQVTPELVK"
BASE COUNT          231 a          162 c          165 g          198 t
ORIGIN      
        1 atgccacgcc ccaaaatcaa tggaattgaa ctaaacaaaa gggatataaa catcagacag
       61 gtttacgtta ctatcaggca tatcacctca gaatcagatg aaaactataa agaaatatct
      121 attatggctt taatatttgt tgataaggat agtaacatga acatgacaaa aggtgcactc
      181 atcctcagcc tttcattttt gcttgccgca tgtagttcaa ttccgcaaaa tatcaaaggc
      241 aataaccaac ctgatattca aaaaagtttt gttgctgttc ataaccagcc ggggttatat
      301 gttggtcaac aagcgcgctt tggtgggaag gttatcaacg ttatcaatgg caaaacggat
      361 acgttgttag aaatcgctgt attaccgttg gatagctatg cgaagcctga tattgaagcc
      421 aactatcagg gccgactgct cgccagacaa agcggcttcc ttgatccagt gaactatcgt
      481 aatcactttg ttaccatcct cggtaccatt cagggtgaac aaccaggctt tatcaataaa
      541 gtcccgtata acttcctgga agtgaatatg cagggcatcc aggtgtggca tttgagagaa
      601 gtggttaata ccacctataa cctgtgggat tacggctatg gtgcattctg gccggaaccg
      661 ggctggggtg cgccttacta caccaatgcg gtgagtcagg taacacctga gctggtcaaa
      721 taacaccacc gaaagatgca gatgctttct cagcat
//
LOCUS       DAZKHF020000679          755 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2201S31241
            SAMN43267800-rid25106413.denovo.683, whole genome shotgun sequence.
ACCESSION   DAZKHF020000679 DAZKHF020000000
VERSION     DAZKHF020000679.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267800
            Sequence Read Archive: SRR30307263
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 755)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 755)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 755)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350581.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350581.2
            Genome Coverage       :: 151x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:21:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,652
            CDSs (total)                      :: 5,557
            Genes (coding)                    :: 5,320
            CDSs (with protein)               :: 5,320
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 237
            CDSs (without protein)            :: 237
            Pseudo Genes (ambiguous residues) :: 0 of 237
            Pseudo Genes (frameshifted)       :: 102 of 237
            Pseudo Genes (incomplete)         :: 130 of 237
            Pseudo Genes (internal stop)      :: 52 of 237
            Pseudo Genes (multiple problems)  :: 41 of 237
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..755
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267800-rid25106413.denovo.683"
                     /serotype="O 26:H11"
                     /isolate="2201S31241"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-01"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            1..267
                     /gene="yddL"
                     /locus_tag="ACHOSC_RS26795"
                     /old_locus_tag="pgaptmp_005359"
                     /pseudo
     CDS             1..267
                     /gene="yddL"
                     /locus_tag="ACHOSC_RS26795"
                     /old_locus_tag="pgaptmp_005359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310103.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_process: GO:0034220 - monoatomic ion transmembrane
                     transport [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="porin"
     gene            306..>755
                     /locus_tag="ACHOSC_RS26800"
                     /old_locus_tag="pgaptmp_005360"
     CDS             306..>755
                     /locus_tag="ACHOSC_RS26800"
                     /old_locus_tag="pgaptmp_005360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucine-rich repeat domain-containing protein"
                     /protein_id="HGU4551073.1"
                     /translation="MITDLILHNHPRMKTITLNDNHIAHLNAKNTTKLEYLNLSNNNL
                     LPTNDIDQLISSKHLWHVLVNVINNDPLAQMQYWTAVRNIIDDTNEVTIDLSGLNLTT
                     QPPGLQNFTSINLDNNQLTHFDATNYDRLVKLSLNSNALESINFPQGR"
BASE COUNT          265 a          156 c          130 g          204 t
ORIGIN      
        1 aaaatagttg cggtggttgt aactggtttg ttagctgcga acgtagcaca cgctgccgaa
       61 gtctataaca aggatggtaa taaactcgac ctttatggca aggttaccgc tctacgttat
      121 tttactgatg ataagcgtga cgatggtgat aaaacttatg cccgtctcgg ctttaaagga
      181 gaaacgcaaa tcaatgatca aatgattggt tttggtcact gggaatatga ttttaaaggc
      241 tataacgatg aagccaacgg ctcgcgcggc aagaatctct gattgagtta agtgcccatc
      301 acaatatgat cactgacctt atattacaca atcatcccag gatgaaaaca atcactttaa
      361 acgacaacca tattgcacat ttaaacgcca aaaacactac aaaactggaa tatttaaact
      421 taagcaataa caatttactg ccaaccaatg acattgatca actaatatca tcaaaacatc
      481 tttggcatgt attagttaac gtcatcaaca atgatccact tgcacaaatg cagtactgga
      541 ctgcagtaag aaatataatt gatgacacta atgaagtgac cattgattta tcaggactta
      601 atttaaccac tcaaccacca gggctgcaaa acttcacctc tatcaatctt gataataacc
      661 aactcacaca ttttgatgca accaactacg atagactcgt aaagctaagt ctgaatagta
      721 atgctcttga gtcaataaat tttcctcaag gcaga
//
LOCUS       DAZKHF020000680          755 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2201S31241
            SAMN43267800-rid25106413.denovo.684, whole genome shotgun sequence.
ACCESSION   DAZKHF020000680 DAZKHF020000000
VERSION     DAZKHF020000680.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267800
            Sequence Read Archive: SRR30307263
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 755)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 755)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 755)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350581.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350581.2
            Genome Coverage       :: 151x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:21:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,652
            CDSs (total)                      :: 5,557
            Genes (coding)                    :: 5,320
            CDSs (with protein)               :: 5,320
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 237
            CDSs (without protein)            :: 237
            Pseudo Genes (ambiguous residues) :: 0 of 237
            Pseudo Genes (frameshifted)       :: 102 of 237
            Pseudo Genes (incomplete)         :: 130 of 237
            Pseudo Genes (internal stop)      :: 52 of 237
            Pseudo Genes (multiple problems)  :: 41 of 237
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..755
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267800-rid25106413.denovo.684"
                     /serotype="O 26:H11"
                     /isolate="2201S31241"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-01"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            complement(<1..162)
                     /gene="kilR"
                     /locus_tag="ACHOSC_RS26805"
                     /old_locus_tag="pgaptmp_005361"
     CDS             complement(<1..162)
                     /gene="kilR"
                     /locus_tag="ACHOSC_RS26805"
                     /old_locus_tag="pgaptmp_005361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309963.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="killing protein KilR"
                     /protein_id="HGU4551074.1"
                     /translation="MIAHHFGTDEIPRQCVTPGDYVLHEGRTYIASANNIKKRKLYIR
                     NLTTKTCITD"
     gene            complement(583..735)
                     /locus_tag="ACHOSC_RS26810"
                     /old_locus_tag="pgaptmp_005362"
     CDS             complement(583..735)
                     /locus_tag="ACHOSC_RS26810"
                     /old_locus_tag="pgaptmp_005362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000379550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1391 family protein"
                     /protein_id="HGU4551075.1"
                     /translation="MDTIDLGNNASLVYGVFPNQDGTFTAMTYTKSKTFKTENGARRW
                     LAKNTS"
BASE COUNT          197 a          181 c          158 g          219 t
ORIGIN      
        1 gtcagtaatg catgtttttg tggtcaggtt acgaatatat agttttcgct ttttaatatt
       61 gtttgccgag gcaatatatg tccggccttc atgaagaaca taatcgccag gagtcacaca
      121 ctgacgtggt atttcatcag ttccgaagtg atgtgcaatc ataattatct ccatttttac
      181 aaatgaactt tgttgatgcg gtgcctggtg cctccaggtg actgcaacca gttaacaatt
      241 acagtcggct ttcccaccca aaccaataag gactaacatg acttttaact gtgccgcgtg
      301 cgcttagccg cattcaccgc atcacaaaat tcactttaaa aagggcggac atcagtcgaa
      361 cttcaagaaa aaactgatgc cgccaagact acacacagca gtgttgttat tcacaaccgg
      421 aggcgcactc ccaccattta aatttaacag acaagaccga ctctttatgg ataccggaaa
      481 tgcgccttcg tgttgtgccc ggttttattt caccacctcc gggctttggt ggcctcggct
      541 atacccctac agcaagaata ttgaattaat ccaataaatg gtttagctgg tatttttggc
      601 aagccagcga cgggcaccat tttcggtttt aaacgttttg cttttggtat acgtcattgc
      661 ggtgaacgtg ccgtcctggt ttggaaacac gccgtacacc agagatgcgt tgttgccaag
      721 atcgatagta tccatgctga cctcatttcc cctta
//
LOCUS       DAZKHF020000681          753 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2201S31241
            SAMN43267800-rid25106413.denovo.685, whole genome shotgun sequence.
ACCESSION   DAZKHF020000681 DAZKHF020000000
VERSION     DAZKHF020000681.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267800
            Sequence Read Archive: SRR30307263
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 753)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 753)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 753)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350581.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350581.2
            Genome Coverage       :: 151x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:21:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,652
            CDSs (total)                      :: 5,557
            Genes (coding)                    :: 5,320
            CDSs (with protein)               :: 5,320
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 237
            CDSs (without protein)            :: 237
            Pseudo Genes (ambiguous residues) :: 0 of 237
            Pseudo Genes (frameshifted)       :: 102 of 237
            Pseudo Genes (incomplete)         :: 130 of 237
            Pseudo Genes (internal stop)      :: 52 of 237
            Pseudo Genes (multiple problems)  :: 41 of 237
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..753
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267800-rid25106413.denovo.685"
                     /serotype="O 26:H11"
                     /isolate="2201S31241"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-01"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            47..271
                     /locus_tag="ACHOSC_RS26815"
                     /old_locus_tag="pgaptmp_005363"
     CDS             47..271
                     /locus_tag="ACHOSC_RS26815"
                     /old_locus_tag="pgaptmp_005363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4551076.1"
                     /translation="MKKLQVTVKPFQGTIPFRVLQHGRVLLEEVFRGKCTECYSRTYE
                     VNATHEEFTVECVMNTDKCRMVSAELQPVC"
     gene            complement(640..>753)
                     /locus_tag="ACHOSC_RS26820"
                     /old_locus_tag="pgaptmp_005364"
     CDS             complement(640..>753)
                     /locus_tag="ACHOSC_RS26820"
                     /old_locus_tag="pgaptmp_005364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3950 domain-containing protein"
                     /protein_id="HGU4551077.1"
                     /translation="AWVIESCRRELAADIKYARQLTIKKNDTQYALRWLFI"
BASE COUNT          207 a          150 c          178 g          218 t
ORIGIN      
        1 tccgcactca gtgtactaag caggcttttt attatccgga ggatgtatga agaaattaca
       61 ggtaacggta aaaccttttc agggaacaat tccgttccgt gttttgcaac atggccgtgt
      121 tctgcttgaa gaggtgttca gaggtaaatg cactgaatgt tattcacgaa catatgaagt
      181 gaatgccacg catgaagaat tcaccgttga gtgtgtgatg aatactgata aatgccgaat
      241 ggtatccgct gaattacagc cagtgtgttg agcgacctta ttatccatgc gcggtattgt
      301 cgccgtattc ctgcattaac agagaccgca gcccgacagg gagactcctc tgcgagagtg
      361 tgcggggata atcaaaaacg atacacaccg gggtttaccg cgtaaacgga gcgcggcgtt
      421 ctcccctcat ggtcgcccgt ccggtgcgat ggtggaagaa actggaatct gttcaataaa
      481 aaaactgccg tgttggagtc acagcagtaa tgtactgatt gggtagaaga ttattattgt
      541 tatgctttat ttttattcta tatggctgat tatttcaatt cggaattaat acagctaatg
      601 tctgtgattt tttataaatt cagcaatata aagaaatagt tatatgaaca gccatcgcag
      661 agcatactgt gtatcattct tttttatagt caactgacgg gcatatttta tgtctgctgc
      721 cagctcccgg cggcaagatt caatgaccca cgc
//
LOCUS       DAZKHF020000682          742 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2201S31241
            SAMN43267800-rid25106413.denovo.686, whole genome shotgun sequence.
ACCESSION   DAZKHF020000682 DAZKHF020000000
VERSION     DAZKHF020000682.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267800
            Sequence Read Archive: SRR30307263
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 742)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 742)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 742)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350581.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350581.2
            Genome Coverage       :: 151x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:21:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,652
            CDSs (total)                      :: 5,557
            Genes (coding)                    :: 5,320
            CDSs (with protein)               :: 5,320
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 237
            CDSs (without protein)            :: 237
            Pseudo Genes (ambiguous residues) :: 0 of 237
            Pseudo Genes (frameshifted)       :: 102 of 237
            Pseudo Genes (incomplete)         :: 130 of 237
            Pseudo Genes (internal stop)      :: 52 of 237
            Pseudo Genes (multiple problems)  :: 41 of 237
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..742
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267800-rid25106413.denovo.686"
                     /serotype="O 26:H11"
                     /isolate="2201S31241"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-01"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            118..645
                     /gene="sodC"
                     /locus_tag="ACHOSC_RS26825"
                     /old_locus_tag="pgaptmp_005365"
     CDS             118..645
                     /gene="sodC"
                     /locus_tag="ACHOSC_RS26825"
                     /old_locus_tag="pgaptmp_005365"
                     /EC_number="1.15.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004784 - superoxide dismutase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase [Cu-Zn] SodC"
                     /protein_id="HGU4551078.1"
                     /translation="MKCKIIAAIAMLTAASCGYAAEQEVPMNLVSADGKEVSIGKITI
                     QETPYGLLFTPALHSLSEGIHGFHVHEKGNCAPALKDGKPVAALSAGGHFDPKNTGKH
                     LGPWSPDGHLGDLPALFVTHDGKANYPVLAPRLNSLKEIKGRSLMLHAGGDNHHDHPE
                     PLGGGGARMACGIIQ"
BASE COUNT          195 a          194 c          181 g          172 t
ORIGIN      
        1 ggtattatta atcgcagtga taggatattg cacagctatt gcgcgatatc atcagaacgc
       61 tgtttgttac cctgtaacca gcaagctcag tctgttaacg gaattaatga gggttttatg
      121 aaatgtaaaa tcattgctgc cattgccatg ctgacagcag catcatgcgg atacgcagca
      181 gaacaggaag tcccaatgaa ccttgtcagt gctgacggaa aagaagtcag cattggaaaa
      241 ataaccattc aggagacccc ctacggtctg ctgttcacac cagcccttca ctctctgtct
      301 gaaggcattc atggttttca tgtgcacgaa aaaggaaatt gcgccccggc actgaaagac
      361 ggaaaaccgg tcgcagcatt atcggctggc ggtcactttg acccgaaaaa caccggcaaa
      421 catcttggcc cctggtctcc ggatggacac ctgggcgacc tccctgcgct gttcgtgacg
      481 catgacggaa aagcgaacta cccggtcctg gccccgagac tgaactcatt aaaagagatt
      541 aaagggcgtt ctctcatgct tcatgctggc ggtgataacc atcatgacca tccggagccc
      601 ctgggcggtg gtggtgcgag aatggcctgc ggcatcattc aataatcagt caggtaaggg
      661 gcgggcccct tacctttatt cctcaggacg ataaatcctt tctccctgaa aagaacggca
      721 catcctccct ctctgagtta at
//
LOCUS       DAZKHF020000683          741 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2201S31241
            SAMN43267800-rid25106413.denovo.687, whole genome shotgun sequence.
ACCESSION   DAZKHF020000683 DAZKHF020000000
VERSION     DAZKHF020000683.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267800
            Sequence Read Archive: SRR30307263
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 741)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 741)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 741)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350581.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350581.2
            Genome Coverage       :: 151x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:21:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,652
            CDSs (total)                      :: 5,557
            Genes (coding)                    :: 5,320
            CDSs (with protein)               :: 5,320
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 237
            CDSs (without protein)            :: 237
            Pseudo Genes (ambiguous residues) :: 0 of 237
            Pseudo Genes (frameshifted)       :: 102 of 237
            Pseudo Genes (incomplete)         :: 130 of 237
            Pseudo Genes (internal stop)      :: 52 of 237
            Pseudo Genes (multiple problems)  :: 41 of 237
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..741
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267800-rid25106413.denovo.687"
                     /serotype="O 26:H11"
                     /isolate="2201S31241"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-01"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            <1..>741
                     /locus_tag="ACHOSC_RS26830"
                     /old_locus_tag="pgaptmp_005366"
     CDS             <1..>741
                     /locus_tag="ACHOSC_RS26830"
                     /old_locus_tag="pgaptmp_005366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="terminase large subunit"
                     /protein_id="HGU4551079.1"
                     /translation="HVKGPMRGRPIELEPWQQFAFACILGFKVKATGRRKYTSAFIEV
                     PRKNAKSTTAAILANWFLIMENGQQDIYTAAVSRDQARIVFDDARQMCLLSRPLRRRV
                     NIQAHKVIHPKSNSLLKPLAAKAATIEGTNPSLSIVDEYHLHPDNGVYSALELGMGAR
                     PEGLLFAITTSGSNVVSACKQHYDYCCQILDGEEVNDSIFVLIYELDDENEVDDPAMW
                     IKANPNIDVSVDREKLASTIQKARGIPS"
BASE COUNT          187 a          192 c          204 g          158 t
ORIGIN      
        1 gcacgtaaaa ggaccgatgc gtggcagacc cattgagctg gagccgtggc agcagttcgc
       61 ctttgcgtgc atcctcggct ttaaggttaa ggccaccgga cggcgcaaat acacgagcgc
      121 ctttatcgaa gtgccgcgca aaaatgcgaa atccacgacc gccgcgattc tggctaactg
      181 gtttctgatt atggagaacg gtcagcagga tatctacacc gcagcggtga gccgtgacca
      241 ggcgcggatc gtctttgatg atgcgcgtca gatgtgcctt ttatcccgac cgttacgcag
      301 gcgggtgaat attcaggcgc ataaggtgat acacccgaaa agcaacagcc tgttaaagcc
      361 gctggcagca aaagcggcaa ccatcgaggg aacaaaccca agcctttcca tcgtggacga
      421 ataccacttg caccccgata acggcgttta ttccgcgctt gagctgggga tgggcgcacg
      481 tcccgaaggg ctgttatttg ccattaccac atcaggcagc aacgtcgttt cagcctgtaa
      541 acagcactat gattattgct gccagatcct ggacggcgaa gaggttaacg attcaatttt
      601 tgtactgatt tacgagctgg acgacgaaaa cgaggttgat gatccggcga tgtggataaa
      661 ggcgaaccct aatattgatg tttccgtcga tcgtgaaaaa ctggcctcaa ccatccagaa
      721 agcgcggggt attccgtcgc a
//
LOCUS       DAZKHF020000684          739 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2201S31241
            SAMN43267800-rid25106413.denovo.688, whole genome shotgun sequence.
ACCESSION   DAZKHF020000684 DAZKHF020000000
VERSION     DAZKHF020000684.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267800
            Sequence Read Archive: SRR30307263
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 739)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 739)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 739)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350581.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350581.2
            Genome Coverage       :: 151x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:21:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,652
            CDSs (total)                      :: 5,557
            Genes (coding)                    :: 5,320
            CDSs (with protein)               :: 5,320
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 237
            CDSs (without protein)            :: 237
            Pseudo Genes (ambiguous residues) :: 0 of 237
            Pseudo Genes (frameshifted)       :: 102 of 237
            Pseudo Genes (incomplete)         :: 130 of 237
            Pseudo Genes (internal stop)      :: 52 of 237
            Pseudo Genes (multiple problems)  :: 41 of 237
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..739
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267800-rid25106413.denovo.688"
                     /serotype="O 26:H11"
                     /isolate="2201S31241"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-01"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            12..230
                     /gene="yiiE"
                     /locus_tag="ACHOSC_RS26835"
                     /old_locus_tag="pgaptmp_005367"
     CDS             12..230
                     /gene="yiiE"
                     /locus_tag="ACHOSC_RS26835"
                     /old_locus_tag="pgaptmp_005367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312842.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0032993 - protein-DNA complex [Evidence
                     IEA];
                     GO_process: GO:0006276 - plasmid maintenance [Evidence
                     IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CopG family transcriptional regulator"
                     /protein_id="HGU4551080.1"
                     /translation="MAMNTVFLHLSEEAIKRLNKLRGWRKVSRSAILREAVEQYLERQ
                     QFPVRKAKGGRQKGEVVGVDDQCKEHKE"
     gene            449..691
                     /gene="yiiF"
                     /locus_tag="ACHOSC_RS26840"
                     /old_locus_tag="pgaptmp_005368"
     CDS             449..691
                     /gene="yiiF"
                     /locus_tag="ACHOSC_RS26840"
                     /old_locus_tag="pgaptmp_005368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709694.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YiiF"
                     /protein_id="HGU4551081.1"
                     /translation="MNSLAGIDMGRILLNLSNEVIKQLDDLEVQRNLPRADLLREAVD
                     QYLINQSQTARTSVPGIWQGCEEDGVEYQRKLREEW"
BASE COUNT          232 a          118 c          189 g          200 t
ORIGIN      
        1 aggtggtggt aatggcgatg aatacggttt ttcttcattt atcagaagag gcaattaaac
       61 ggctgaacaa acttcggggc tggcgtaagg tttcacgatc tgcaatttta cgcgaagcgg
      121 ttgagcaata tctggagcgg cagcaatttc cggtgcgtaa agcaaaggga ggcaggcaaa
      181 agggggaggt agttggtgtt gatgatcagt gtaaggagca taaggaatga aaaaaatttt
      241 tcattcttga atataaaaaa acagatgccc ttattctggt attaatacaa ggctgtttta
      301 cttgaactta taataactgc aactgttaca tcgtatctgg aaaacgcctc gcaaaaataa
      361 aaaatgatgc gtaaatgagc tttctttaaa gattcggcaa gggtactttt ctctccatag
      421 attcaaaaat gatattggcg agatatttat gaactctctg gcaggtatag atatgggcag
      481 aattttactc aatttatcga atgaggtgat taagcaactt gatgatctcg aggtgcagcg
      541 taatcttcct cgtgcggacc tattaaggga agcggtagat caatacctga taaatcaatc
      601 gcaaacagca agaaccagtg ttcctggcat ctggcaaggg tgtgaggaag atggtgtcga
      661 atatcagcgt aagctgcgcg aggaatggta atgggaaaaa atgatgtcaa tcagattgct
      721 gacaacgtgc gcgttgttc
//
LOCUS       DAZKHF020000685          736 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2201S31241
            SAMN43267800-rid25106413.denovo.689, whole genome shotgun sequence.
ACCESSION   DAZKHF020000685 DAZKHF020000000
VERSION     DAZKHF020000685.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267800
            Sequence Read Archive: SRR30307263
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 736)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 736)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 736)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350581.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350581.2
            Genome Coverage       :: 151x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:21:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,652
            CDSs (total)                      :: 5,557
            Genes (coding)                    :: 5,320
            CDSs (with protein)               :: 5,320
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 237
            CDSs (without protein)            :: 237
            Pseudo Genes (ambiguous residues) :: 0 of 237
            Pseudo Genes (frameshifted)       :: 102 of 237
            Pseudo Genes (incomplete)         :: 130 of 237
            Pseudo Genes (internal stop)      :: 52 of 237
            Pseudo Genes (multiple problems)  :: 41 of 237
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..736
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267800-rid25106413.denovo.689"
                     /serotype="O 26:H11"
                     /isolate="2201S31241"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-01"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            complement(<1..198)
                     /locus_tag="ACHOSC_RS26845"
                     /old_locus_tag="pgaptmp_005369"
     CDS             complement(<1..198)
                     /locus_tag="ACHOSC_RS26845"
                     /old_locus_tag="pgaptmp_005369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308874.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M85 family metallopeptidase"
                     /protein_id="HGU4551082.1"
                     /translation="MYKTILSINMKIPVLQPGFNFFSPAGYSAAVAPNRAENAYADYV
                     LDIGKRIPLSAADLSNVYESVI"
     gene            complement(425..>736)
                     /locus_tag="ACHOSC_RS26850"
                     /old_locus_tag="pgaptmp_005370"
     CDS             complement(425..>736)
                     /locus_tag="ACHOSC_RS26850"
                     /old_locus_tag="pgaptmp_005370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001429641.1"
                     /note="secreted by salmonella pathogenicity island 1;
                     involved in survival of Salmonella in host cells;
                     interferes with host cell ubiquination pathway; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="E3 ubiquitin--protein ligase"
                     /protein_id="HGU4551083.1"
                     /translation="TEKAEILFCLGAVFCKYSSSDIFGTEHESPEILRRYANGLLEEA
                     YKLASEIFNKREFYDDIRDRLQGRNNAFSCTAVLADILTSHAKGNFTDIFEQYYPLAW
                     R"
BASE COUNT          235 a          167 c          131 g          203 t
ORIGIN      
        1 aattacactt tcgtatacgt tgcttaaatc tgctgcggaa agcggtattc gcttgcctat
       61 atccaaaacg taatccgcat aggcattttc agcacgatta ggagcaacgg cagcagagta
      121 tccagcaggg gaaaaaaagt tgaagccagg ttggagaacg ggaattttca tattgatact
      181 taatatggtt ttatatattt cagtgccacc aattcactag cccagacgat aacggacagc
      241 atagctttta tccctgcaag gcaggtgttc caacgagtaa caggaacgtt taaagctccc
      301 atacacatgt gagctaatga caccacaatg gtaaaccttc cttatcgtag caaagcttac
      361 caagtttaat gagctgtctg ccctgtgatt tgcaatatat attggcatca cgtagcaaca
      421 gacatcaacg ccatgctaat ggataatact gctcaaaaat atcagtaaaa tttccctttg
      481 catggcttgt taatatatca gctaaaaccg cagtgcatga aaatgcatta ttccgtcctt
      541 gcaacctgtc cctgatatca tcataaaact ctcttttgtt aaatatctca gatgccagtt
      601 tatatgcctc ttccaaaaga ccatttgcat aacgacgcag gatctcagga gactcatgct
      661 ccgttccaaa tatatcagat gacgagtact tacaaaaaac agcacctaaa caaaataata
      721 tctcagcctt ttccgt
//
LOCUS       DAZKHF020000686          734 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2201S31241
            SAMN43267800-rid25106413.denovo.690, whole genome shotgun sequence.
ACCESSION   DAZKHF020000686 DAZKHF020000000
VERSION     DAZKHF020000686.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267800
            Sequence Read Archive: SRR30307263
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 734)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 734)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 734)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350581.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350581.2
            Genome Coverage       :: 151x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:21:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,652
            CDSs (total)                      :: 5,557
            Genes (coding)                    :: 5,320
            CDSs (with protein)               :: 5,320
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 237
            CDSs (without protein)            :: 237
            Pseudo Genes (ambiguous residues) :: 0 of 237
            Pseudo Genes (frameshifted)       :: 102 of 237
            Pseudo Genes (incomplete)         :: 130 of 237
            Pseudo Genes (internal stop)      :: 52 of 237
            Pseudo Genes (multiple problems)  :: 41 of 237
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..734
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267800-rid25106413.denovo.690"
                     /serotype="O 26:H11"
                     /isolate="2201S31241"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-01"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            complement(<1..591)
                     /gene="pphA"
                     /locus_tag="ACHOSC_RS26855"
                     /old_locus_tag="pgaptmp_005371"
     CDS             complement(<1..591)
                     /gene="pphA"
                     /locus_tag="ACHOSC_RS26855"
                     /old_locus_tag="pgaptmp_005371"
                     /EC_number="3.1.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416352.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein-serine/threonine phosphatase"
                     /protein_id="HGU4551084.1"
                     /translation="MKQPAPVYQRIAGHQWRHIWLSGDIHGCLEQLRRKLWHCRFDPW
                     RDLLISVGDVIDRGPQSLRCLQLLEQHWVCAVRGNHEQMAMDALASQQMSLWLMNGGD
                     WFIALADNQQKQAKTALEKCQHLPFILEVHSRTGKHVIAHADYPDDVYEWQKDVDLHQ
                     VLWSRSRLGERQKGQGITGADHFWFGHTPLRHRVDIG"
BASE COUNT          215 a          189 c          146 g          184 t
ORIGIN      
        1 gccaatatcc acgcgatgtc gcaacggtgt atgaccaaac cagaaatgat cagcacctgt
       61 aattccctgc cctttttggc gttcacctaa tcgcgagcgg ctccacaaga cctgatgcaa
      121 atcaacgtcc ttttgccatt cataaacatc atctggataa tcggcatgag caataacatg
      181 tttgccggta cgactgtgta cttcaagaat aaagggcaaa tgctgacatt tttccagcgc
      241 cgttttcgct tgtttctgtt gattatctgc cagcgcaata aaccagtcgc cgccattcat
      301 caaccacaaa gacatctgct gggatgccag cgcatccatc gccatctgtt catgattgcc
      361 tcttaccgca caaacccaat gttgttccag taactgcaga caacgtaaac tttgcggccc
      421 acgatcgata acgtctccta ctgagataag taaatctcgc cacggatcaa aacgacaatg
      481 ccataatttg cggcgcaact gctcaagaca accgtgtata tcgccagaaa gccagatatg
      541 tcgccattga tgacccgcaa ttctctgata aacgggcgca ggctgtttca tcaatatttt
      601 cctcccgcgc taaagatcac ataatcttaa caagaatgtt aaaaaacgct ggactcagac
      661 agtagagtgt gtgttatggt tgactataaa gtcagcgaag gaaatgcttc tggcttttaa
      721 cagataaaaa gaga
//
LOCUS       DAZKHF020000687          733 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2201S31241
            SAMN43267800-rid25106413.denovo.691, whole genome shotgun sequence.
ACCESSION   DAZKHF020000687 DAZKHF020000000
VERSION     DAZKHF020000687.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267800
            Sequence Read Archive: SRR30307263
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 733)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 733)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 733)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350581.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350581.2
            Genome Coverage       :: 151x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:21:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,652
            CDSs (total)                      :: 5,557
            Genes (coding)                    :: 5,320
            CDSs (with protein)               :: 5,320
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 237
            CDSs (without protein)            :: 237
            Pseudo Genes (ambiguous residues) :: 0 of 237
            Pseudo Genes (frameshifted)       :: 102 of 237
            Pseudo Genes (incomplete)         :: 130 of 237
            Pseudo Genes (internal stop)      :: 52 of 237
            Pseudo Genes (multiple problems)  :: 41 of 237
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..733
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267800-rid25106413.denovo.691"
                     /serotype="O 26:H11"
                     /isolate="2201S31241"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-01"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            <1..>733
                     /locus_tag="ACHOSC_RS26860"
                     /old_locus_tag="pgaptmp_005372"
     CDS             <1..>733
                     /locus_tag="ACHOSC_RS26860"
                     /old_locus_tag="pgaptmp_005372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="RHS element protein"
                     /protein_id="HGU4551085.1"
                     /translation="VYDRSNTQVRSFTYDDKYRGRMVAHRHTSRPEIRYRYDSDGRVT
                     EQLNPAGLSYTYQYEKDHITITDSLDRREVLHTQGEAGLKRVVKKEHADGSVTQSQFD
                     AVGRLRTQTDAAGRTTEYSPDVVTGLITRITTPDGRASAFYYNHHSQLTSATGPDGLE
                     IRREYDELGRLIQETAPDGDITRYRYDNPHSDLPCATEDATGSRKTMTWSRYAQLLSF
                     TDCSGYVTRYDHDRFGQMTAVHREEG"
BASE COUNT          186 a          211 c          219 g          117 t
ORIGIN      
        1 agtgtatgac cgcagcaata cacaggtgcg cagctttact tacgatgata aataccgggg
       61 ccggatggtg gcgcaccgtc acacgagccg accggaaatc cgttaccgtt acgacagcga
      121 cgggcgggtg acagaacagc taaacccggc aggcttaagc tacacgtatc agtatgagaa
      181 agaccacatc accatcaccg acagcctgga ccgccgtgaa gtgctgcaca cgcagggcga
      241 agccgggctg aagcgggtgg tgaaaaagga acacgcggac ggcagcgtca cgcagagtca
      301 gtttgacgcc gtgggcaggc tcaggacaca gacggatgcc gcaggccgga caacagaata
      361 cagcccggat gtggtgacgg gcctcatcac gcgcatcacc acgccggatg gcagggcatc
      421 ggcgttttac tataaccacc acagccagtt aacgtcagcc accgggcctg acgggctgga
      481 aatacgccgg gaatatgatg aattgggccg tctgattcag gaaactgccc ctgacggcga
      541 tatcacccgc taccgttatg ataatccaca cagtgactta ccctgcgcaa cggaagatgc
      601 caccggcagc cggaaaacca tgacgtggag ccgttacgct cagttgctga gcttcaccga
      661 ctgttccggt tatgtaaccc gttatgacca tgaccgcttc gggcagatga cggcggtgca
      721 ccgcgaggaa ggc
//
LOCUS       DAZKHF020000688          733 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2201S31241
            SAMN43267800-rid25106413.denovo.692, whole genome shotgun sequence.
ACCESSION   DAZKHF020000688 DAZKHF020000000
VERSION     DAZKHF020000688.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267800
            Sequence Read Archive: SRR30307263
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 733)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 733)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 733)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350581.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350581.2
            Genome Coverage       :: 151x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:21:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,652
            CDSs (total)                      :: 5,557
            Genes (coding)                    :: 5,320
            CDSs (with protein)               :: 5,320
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 237
            CDSs (without protein)            :: 237
            Pseudo Genes (ambiguous residues) :: 0 of 237
            Pseudo Genes (frameshifted)       :: 102 of 237
            Pseudo Genes (incomplete)         :: 130 of 237
            Pseudo Genes (internal stop)      :: 52 of 237
            Pseudo Genes (multiple problems)  :: 41 of 237
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..733
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267800-rid25106413.denovo.692"
                     /serotype="O 26:H11"
                     /isolate="2201S31241"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-01"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            <1..>733
                     /locus_tag="ACHOSC_RS26865"
                     /old_locus_tag="pgaptmp_005373"
     CDS             <1..>733
                     /locus_tag="ACHOSC_RS26865"
                     /old_locus_tag="pgaptmp_005373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004197 - cysteine-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YopT-type cysteine protease domain-containing
                     protein"
                     /protein_id="HGU4551086.1"
                     /translation="PSITTIIKKTEYHGHQLTNAMTVASVVKPYDFSNLGAINSIDKS
                     VSDIPALHTIVEQAKYNLLSWNDFYNTHASIWDTIARQHKSTNIEFHPQSLLFDRDSK
                     GKCLGLSLLYLDTGGYGSRYQKLRHNIETASALYQTKYNDNIKLSNSDDFFLRKTQRI
                     ITMSNELGNNRLKNSQLEILELKDPVLTEGILYQRRISSLLITTEYHSLALQQVSSFW
                     RVTDPNFGHCDFHSLAQALTFIKNIT"
BASE COUNT          270 a          126 c          117 g          220 t
ORIGIN      
        1 cccagtataa caacaataat aaaaaaaaca gaatatcatg gacatcagtt aacaaatgct
       61 atgacagttg cttcagtggt taagccatat gactttagta atcttggtgc cataaatagt
      121 atagataagt ccgtttctga tatacctgca ttgcatacaa ttgttgaaca agcgaagtat
      181 aatttacttt catggaatga tttttataat actcatgcgt caatatggga tacaatagca
      241 aggcagcata aaagtacaaa tatcgaattt cacccacaaa gtcttctttt tgatagagat
      301 tcgaaaggaa agtgccttgg tttgtctctc ctgtatcttg atacaggagg ttatggaagc
      361 agatatcaaa aactgagaca caatattgaa actgccagcg cattgtatca aacaaaatat
      421 aatgacaata ttaaattatc taatagcgat gatttctttt taagaaaaac acaacgtatc
      481 attacaatgt ccaatgagtt aggcaataat agattaaaaa actcacagtt agaaatccta
      541 gaattgaagg accctgtact aactgaaggt atattatacc agcgtcgaat atcatctctt
      601 ttaataacaa cagaatatca ctcactcgcg ttacaacaag tatcttcttt ttggagagta
      661 accgatccta attttgggca ttgtgacttc cattccttag cacaggcttt aacgtttata
      721 aaaaatataa cga
//
LOCUS       DAZKHF020000689          727 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2201S31241
            SAMN43267800-rid25106413.denovo.693, whole genome shotgun sequence.
ACCESSION   DAZKHF020000689 DAZKHF020000000
VERSION     DAZKHF020000689.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267800
            Sequence Read Archive: SRR30307263
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 727)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 727)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 727)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350581.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350581.2
            Genome Coverage       :: 151x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:21:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,652
            CDSs (total)                      :: 5,557
            Genes (coding)                    :: 5,320
            CDSs (with protein)               :: 5,320
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 237
            CDSs (without protein)            :: 237
            Pseudo Genes (ambiguous residues) :: 0 of 237
            Pseudo Genes (frameshifted)       :: 102 of 237
            Pseudo Genes (incomplete)         :: 130 of 237
            Pseudo Genes (internal stop)      :: 52 of 237
            Pseudo Genes (multiple problems)  :: 41 of 237
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..727
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267800-rid25106413.denovo.693"
                     /serotype="O 26:H11"
                     /isolate="2201S31241"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-01"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            <1..>727
                     /locus_tag="ACHOSC_RS26870"
                     /old_locus_tag="pgaptmp_005374"
     CDS             <1..>727
                     /locus_tag="ACHOSC_RS26870"
                     /old_locus_tag="pgaptmp_005374"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="S49 family peptidase"
                     /protein_id="HGU4551087.1"
                     /translation="RLTDAVSGDSLTAGEAPAALALSGDDDGPRQARSYQVMNGIAVL
                     PVSGTLVSRTRALQPYSGMTGYNGIIARLQQAASDPMVDGILLDMDTPGGMVAGAFDC
                     ADIIARVRDIKPVWALANDMNCSAGQLLASAASRRLVTQTARTGSIGVMMAHSNYGAA
                     LEKQGVEITLIYSGSHKVDGNPYSHLPGDVRETLQSRMDATRRMFAQKVSAYTGLSVQ
                     AVLDTEAAVYSGQEAIDAGLADEL"
BASE COUNT          143 a          210 c          241 g          133 t
ORIGIN      
        1 ccgcctgacg gatgcagtat ccggcgacag cctgactgcc ggagaggcac ccgcggcgct
       61 ggcgttatcc ggtgatgatg acggaccacg acaggcccgg agttatcagg tcatgaacgg
      121 catcgccgtg ctgccggtgt ccggtacgct ggtcagccgg acgcgggcgc tgcagccgta
      181 ttcggggatg accggttaca acggcattat cgcccgtctg caacaggctg ccagcgaccc
      241 gatggtggac ggcattctgc tcgatatgga cacaccgggc ggaatggtgg cgggagcatt
      301 tgactgtgct gacatcatcg cccgtgtgcg tgacataaaa ccggtatggg cgctggccaa
      361 cgacatgaac tgcagtgcag gtcagctgct tgccagcgcc gcctcccggc gtctggtcac
      421 gcagaccgcc cggacaggct ccatcggcgt catgatggct cacagtaatt acggtgctgc
      481 cctggagaaa cagggcgtgg aaatcacgct gatttacagc ggcagccata aggtggatgg
      541 caacccctac agccatctac cgggtgatgt ccgggaaaca ctgcagtccc ggatggatgc
      601 aacccgccgg atgtttgcgc agaaggtgtc ggcatatacc ggcctgtccg tgcaggctgt
      661 gctggatacc gaggctgcag tgtacagcgg tcaggaggcc attgatgccg gactggctga
      721 tgaactt
//
LOCUS       DAZKHF020000690          720 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2201S31241
            SAMN43267800-rid25106413.denovo.694, whole genome shotgun sequence.
ACCESSION   DAZKHF020000690 DAZKHF020000000
VERSION     DAZKHF020000690.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267800
            Sequence Read Archive: SRR30307263
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 720)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 720)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 720)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350581.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350581.2
            Genome Coverage       :: 151x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:21:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,652
            CDSs (total)                      :: 5,557
            Genes (coding)                    :: 5,320
            CDSs (with protein)               :: 5,320
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 237
            CDSs (without protein)            :: 237
            Pseudo Genes (ambiguous residues) :: 0 of 237
            Pseudo Genes (frameshifted)       :: 102 of 237
            Pseudo Genes (incomplete)         :: 130 of 237
            Pseudo Genes (internal stop)      :: 52 of 237
            Pseudo Genes (multiple problems)  :: 41 of 237
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..720
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267800-rid25106413.denovo.694"
                     /serotype="O 26:H11"
                     /isolate="2201S31241"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-01"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            complement(43..273)
                     /gene="ynfN"
                     /locus_tag="ACHOSC_RS26875"
                     /old_locus_tag="pgaptmp_005375"
     CDS             complement(43..273)
                     /gene="ynfN"
                     /locus_tag="ACHOSC_RS26875"
                     /old_locus_tag="pgaptmp_005375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000720962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YnfN"
                     /protein_id="HGU4551088.1"
                     /translation="MKKIIVFFNSEPAVVVPAMTGVNTIMREYPNGEKTHLTVMAAGF
                     PSLTGDHKVIYVAADRHVTSEEILEAAIRLLS"
     gene            complement(278..343)
                     /gene="ynfR"
                     /locus_tag="ACHOSC_RS26880"
                     /old_locus_tag="pgaptmp_005376"
     CDS             complement(278..343)
                     /gene="ynfR"
                     /locus_tag="ACHOSC_RS26880"
                     /old_locus_tag="pgaptmp_005376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="small protein YnfR"
                     /protein_id="HGU4551089.1"
                     /translation="MKAPSGAFLLGIYSMDTHILR"
     gene            complement(346..534)
                     /gene="ynfQ"
                     /locus_tag="ACHOSC_RS26885"
                     /old_locus_tag="pgaptmp_005377"
     CDS             complement(346..534)
                     /gene="ynfQ"
                     /locus_tag="ACHOSC_RS26885"
                     /old_locus_tag="pgaptmp_005377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HGU4551090.1"
                     /translation="MTNHIHFRCPCCHGSQYRTSSFDVSDMNPFGAKCIFCKSMMITF
                     DNISQYLNASRLSLDLKK"
     gene            complement(545..>720)
                     /gene="cspI"
                     /locus_tag="ACHOSC_RS26890"
                     /old_locus_tag="pgaptmp_005378"
     CDS             complement(545..>720)
                     /gene="cspI"
                     /locus_tag="ACHOSC_RS26890"
                     /old_locus_tag="pgaptmp_005378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="cold shock protein CspI"
                     /protein_id="HGU4551091.1"
                     /translation="PEKGFGFITPKDGSKDVFVHFSAIQSNDFKTLTENQEVEFGIEN
                     GPKGPAAVHVVAL"
BASE COUNT          223 a          149 c          132 g          216 t
ORIGIN      
        1 acaaagagaa ttttcctgaa ttatcaatac aatagcatca aatcaactca agagccttat
       61 tgctgcttcc agaatttctt ctgaagtaac atgtcgatcc gcggctacat aaatgacttt
      121 atgatctccg gtcagagatg gaaaccctgc ggccattaca gtaaggtgtg ttttttcgcc
      181 atttggatat tcacgcatga tggtgttaac tccagtcatc gctggcacta ccactgctgg
      241 ttcagagtta aaaaaaacta tgattttttt catgatgtta ccgtagtatg tgagtatcca
      301 tcgaatagat accaagcaaa aaagctcccg aaggagcctt cattttcact tttttaaatc
      361 caacgacaga cggctggcat ttaagtattg tgaaatatta tcaaatgtaa tcatcattga
      421 tttacaaaag atacattttg ccccgaaagg attcatgtca gaaacatcaa aagatgatgt
      481 tctatactgg gaaccatgac aacacgggca tctaaagtga atatggtttg taatattgtc
      541 tacctcaaag cgccactaca tgaacagcgg caggaccttt aggtccgttc tcaataccaa
      601 attcaacttc ctgattctca gttaatgttt tgaaatcgtt gctctgaatt gctgagaaat
      661 ggacaaacac atctttgctg ccatctttcg gcgtgatgaa accaaaacct ttttcagggt
//
LOCUS       DAZKHF020000691          719 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2201S31241
            SAMN43267800-rid25106413.denovo.695, whole genome shotgun sequence.
ACCESSION   DAZKHF020000691 DAZKHF020000000
VERSION     DAZKHF020000691.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267800
            Sequence Read Archive: SRR30307263
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 719)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 719)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 719)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350581.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350581.2
            Genome Coverage       :: 151x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:21:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,652
            CDSs (total)                      :: 5,557
            Genes (coding)                    :: 5,320
            CDSs (with protein)               :: 5,320
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 237
            CDSs (without protein)            :: 237
            Pseudo Genes (ambiguous residues) :: 0 of 237
            Pseudo Genes (frameshifted)       :: 102 of 237
            Pseudo Genes (incomplete)         :: 130 of 237
            Pseudo Genes (internal stop)      :: 52 of 237
            Pseudo Genes (multiple problems)  :: 41 of 237
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..719
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267800-rid25106413.denovo.695"
                     /serotype="O 26:H11"
                     /isolate="2201S31241"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-01"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            complement(<1..78)
                     /gene="hokD"
                     /locus_tag="ACHOSC_RS26895"
                     /old_locus_tag="pgaptmp_005379"
     CDS             complement(<1..78)
                     /gene="hokD"
                     /locus_tag="ACHOSC_RS26895"
                     /old_locus_tag="pgaptmp_005379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin HokD"
                     /protein_id="HGU4551092.1"
                     /translation="MKQQKAMLIALIVICLTVIVTALVTR"
     gene            complement(325..429)
                     /locus_tag="ACHOSC_RS26900"
                     /old_locus_tag="pgaptmp_005380"
     CDS             complement(325..429)
                     /locus_tag="ACHOSC_RS26900"
                     /old_locus_tag="pgaptmp_005380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4551093.1"
                     /translation="MSDDISLAMEGALAVVAVVGVYCLVVFLMDRLGN"
     gene            complement(545..>719)
                     /locus_tag="ACHOSC_RS26905"
                     /old_locus_tag="pgaptmp_005381"
     CDS             complement(545..>719)
                     /locus_tag="ACHOSC_RS26905"
                     /old_locus_tag="pgaptmp_005381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000207992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF551 domain-containing protein"
                     /protein_id="HGU4551094.1"
                     /translation="TAVLVAVEFDRKGDWRMKWATYIPGHPDANDGWIIPGASWKPSH
                     WMPLPEPPQEVRQ"
BASE COUNT          182 a          207 c          143 g          187 t
ORIGIN      
        1 cctcgttacc agtgccgtca ctatgacggt taaacagatg acgatcaggg cgattaacat
       61 cgccttttgc tgcttcatag cctgcttctc cttgcctttc ggcacgtaag aggctaacct
      121 acatttgtga gacatagatt gggcctcaga ttaatgttaa gcgtcttgca ggacgcgtaa
      181 tgttaactgg ggcttttctc tatctgcctt ttggtgttca tgcctgagac agatagcctc
      241 aagcacccgc agcaattcta cttaactctc cttttcccgc aaaccgtttt tacccgatat
      301 gggaattccc atatcgtaat gaattcagtt ccctagtcga tccatcaaaa acacaaccag
      361 gcagtaaacg cccacaacag caacaacagc cagcgcacct tccattgcca gtgatatatc
      421 atccgacata ttccctcctt tggtgttaat cccggcgaac gtttttaccc ccaccgacaa
      481 ataacatata ctaaaaaagc gatagccata gcaacgcctg taattgcaaa tgcttcaggc
      541 cagttcattg gcgcacctcc tgcggcggtt ctggtagcgg catccagtgt gacggtttcc
      601 acgacgcacc aggaattatc cacccatcat tagcgtcagg atgacccggg atgtaagtcg
      661 cccatttcat tcgccagtca cctttcctgt caaactccac ggcaacaaga acggctgtt
//
LOCUS       DAZKHF020000692          719 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2201S31241
            SAMN43267800-rid25106413.denovo.696, whole genome shotgun sequence.
ACCESSION   DAZKHF020000692 DAZKHF020000000
VERSION     DAZKHF020000692.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267800
            Sequence Read Archive: SRR30307263
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 719)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 719)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 719)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350581.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350581.2
            Genome Coverage       :: 151x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:21:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,652
            CDSs (total)                      :: 5,557
            Genes (coding)                    :: 5,320
            CDSs (with protein)               :: 5,320
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 237
            CDSs (without protein)            :: 237
            Pseudo Genes (ambiguous residues) :: 0 of 237
            Pseudo Genes (frameshifted)       :: 102 of 237
            Pseudo Genes (incomplete)         :: 130 of 237
            Pseudo Genes (internal stop)      :: 52 of 237
            Pseudo Genes (multiple problems)  :: 41 of 237
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..719
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267800-rid25106413.denovo.696"
                     /serotype="O 26:H11"
                     /isolate="2201S31241"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-01"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     repeat_region   129..702
                     /inference="COORDINATES: alignment:crt:1.2"
                     /inference="COORDINATES: alignment:pilercr:v1.02"
                     /rpt_family="CRISPR"
                     /rpt_type=direct
                     /rpt_unit_range=190..214
                     /rpt_unit_seq="cggtttatccccgctggcgcgggga"
BASE COUNT          151 a          194 c          217 g          157 t
ORIGIN      
        1 ttgtatcgat aattgtgaac ctctctggca tggagaacta ttttgaacat gaggtgttac
       61 gtggatatgt tgcttattac aagtactgct aatataaaaa cttgagaaag agataacggg
      121 ttatatggtg gtttatcccc gctggcgcgg ggagctcaac atcggaaacg gcttcgcggc
      181 ggcggcgtcc ggtttatccc cgctggcgcg gggaactcga cagaacggcc tcagtagtct
      241 cgtcaggctc cggtttatcc ccgctggcgc ggggagctct acgtgaagaa tatttgcaac
      301 acccgcaaga acggtttatc cccgctggcg cggggagctc ggcatagcca ggctgatccg
      361 gcgacggcct tacggtttat ccccgctggc gcggggaaca caatcgtgtg taaattcgcg
      421 gcggctccac tggcggttta tccccgctgg cgcggggaac actggtcgaa atatagacag
      481 catgttccgt accacggttt atccccgctg gcgcggggaa cacacactat ccgggcggta
      541 ttacgcccaa atatccggtt tatccccgct ggcgcgggga acacctgccg ggtgaaacca
      601 ctcgcggcag atcttgcggt ttatccccgc tggcgcgggg aacacactac tgtcggtagc
      661 tgggaggatg aggagatcgg tttatccccg ctggcgcggg gaacactcta aacataacc
//
LOCUS       DAZKHF020000693          718 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2201S31241
            SAMN43267800-rid25106413.denovo.697, whole genome shotgun sequence.
ACCESSION   DAZKHF020000693 DAZKHF020000000
VERSION     DAZKHF020000693.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267800
            Sequence Read Archive: SRR30307263
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 718)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 718)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 718)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350581.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350581.2
            Genome Coverage       :: 151x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:21:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,652
            CDSs (total)                      :: 5,557
            Genes (coding)                    :: 5,320
            CDSs (with protein)               :: 5,320
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 237
            CDSs (without protein)            :: 237
            Pseudo Genes (ambiguous residues) :: 0 of 237
            Pseudo Genes (frameshifted)       :: 102 of 237
            Pseudo Genes (incomplete)         :: 130 of 237
            Pseudo Genes (internal stop)      :: 52 of 237
            Pseudo Genes (multiple problems)  :: 41 of 237
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..718
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267800-rid25106413.denovo.697"
                     /serotype="O 26:H11"
                     /isolate="2201S31241"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-01"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            105..701
                     /gene="fimE"
                     /locus_tag="ACHOSC_RS26910"
                     /old_locus_tag="pgaptmp_005382"
     CDS             105..701
                     /gene="fimE"
                     /locus_tag="ACHOSC_RS26910"
                     /old_locus_tag="pgaptmp_005382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418733.1"
                     /note="upgraded from regulatory protein to switch DNA
                     invertase; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbria switch DNA invertase FimE"
                     /protein_id="HGU4551095.1"
                     /translation="MSKRRYLTGKEVQAMMQAVCYGATGARDYCLILLAYRHGMRISE
                     LLDLHYQDLDLNEGRINIRRLKNGFSTVHPLRFDEREAVERWTQERANWKGADRTDAI
                     FISRRGSRLSRQQAYRIIRDAGIEAGTVTQTHPHMLRHACGYELAERGADTRLIQDYL
                     GHRNIRHTVRYTASNAARFAGLWERNNLINEKLKREEV"
BASE COUNT          184 a          141 c          178 g          215 t
ORIGIN      
        1 cacttcgtgt gtggttatct ttttatctat tgggctaatt ttgaccgatt gaggtttcct
       61 ataggtattc attcaaatat atctcagtca ggagtactac tattgtgagt aaacgtcgtt
      121 atcttaccgg taaagaagtt caggccatga tgcaggcggt ttgttacggg gcaacgggag
      181 ccagagatta ttgtcttatt ctgttggcat atcggcatgg gatgcgtatt agtgaactgc
      241 ttgatctgca ttatcaggat cttgacctta atgaaggtcg aataaatatt cgccgactga
      301 agaacggatt ttctaccgtt cacccgttac gttttgatga gcgtgaagcc gtggaacgct
      361 ggacccagga acgtgctaac tggaaaggcg ctgaccggac cgacgctata tttatttctc
      421 gccgtgggag tcggctttct cgccagcagg cctatcgcat tattcgcgat gccggtattg
      481 aagctggaac cgtaacgcag actcatcctc atatgttaag gcatgcttgc ggttatgaac
      541 tggcggagcg tggtgcagat actcgtttaa ttcaggatta tctcgggcat cgaaatattc
      601 gccatactgt acgttatacc gccagtaatg ctgctcgttt tgccggatta tgggaaagaa
      661 ataatctcat aaacgaaaaa ttaaaaagag aagaggtttg atttaactca ttgataat
//
LOCUS       DAZKHF020000694          718 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2201S31241
            SAMN43267800-rid25106413.denovo.698, whole genome shotgun sequence.
ACCESSION   DAZKHF020000694 DAZKHF020000000
VERSION     DAZKHF020000694.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267800
            Sequence Read Archive: SRR30307263
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 718)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 718)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 718)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350581.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350581.2
            Genome Coverage       :: 151x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:21:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,652
            CDSs (total)                      :: 5,557
            Genes (coding)                    :: 5,320
            CDSs (with protein)               :: 5,320
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 237
            CDSs (without protein)            :: 237
            Pseudo Genes (ambiguous residues) :: 0 of 237
            Pseudo Genes (frameshifted)       :: 102 of 237
            Pseudo Genes (incomplete)         :: 130 of 237
            Pseudo Genes (internal stop)      :: 52 of 237
            Pseudo Genes (multiple problems)  :: 41 of 237
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..718
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267800-rid25106413.denovo.698"
                     /serotype="O 26:H11"
                     /isolate="2201S31241"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-01"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            114..698
                     /gene="yadN"
                     /locus_tag="ACHOSC_RS26915"
                     /old_locus_tag="pgaptmp_005383"
     CDS             114..698
                     /gene="yadN"
                     /locus_tag="ACHOSC_RS26915"
                     /old_locus_tag="pgaptmp_005383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HGU4551096.1"
                     /translation="MSKKLGFALSGLMLAMVAGTASADMDGGQLNISGLVVDNTCETR
                     VDGGNKDGLILLQTATVGEIDAGVLNDTVGAKAKPFSITVDCSKANPNPGSTAKMTFG
                     SVFFGNSKGTLNNDMSINNPSDGVNIALHNIDGSTIKQVQINNPGDVYTKALDATTKS
                     AVYDFKASYVRAVADQTATAGYVKTNTAYTITYQ"
BASE COUNT          209 a          149 c          147 g          213 t
ORIGIN      
        1 gtaacaaata tcattgctca ttaatatcta gcatctgctt ataacatttg tgtcatgaat
       61 agtcattctt caaaccagta gtctgactaa tggaatttta ttcaaggatt tttatgtcta
      121 aaaaattagg ttttgccctg tctggtctta tgctggcaat ggttgcaggt actgcttctg
      181 ctgatatgga tggtggtcag ttaaatatca gtggtctggt tgttgataac acctgtgaaa
      241 cccgcgttga cggcggtaat aaagatggtc tgatcctgct gcaaactgca actgttggtg
      301 aaatcgatgc tggcgtgctg aatgacaccg ttggcgctaa agccaaacct ttcagcatca
      361 ccgttgactg cagcaaagca aatccgaatc caggtagtac agctaaaatg accttcggtt
      421 ctgttttctt tggtaatagc aaaggaacgc tgaacaacga catgtctatt aacaacccgt
      481 ctgacggcgt gaatatcgct ctgcacaata ttgatggttc cactatcaaa caggttcaaa
      541 ttaacaaccc tggcgatgtg tatactaaag ccctggatgc aacgacaaaa tctgctgttt
      601 atgattttaa agcgtcttac gttcgtgccg tcgcagacca aacagcaact gctggttatg
      661 taaaaactaa cactgcatac accattactt atcagtaagt attcaccaat taaattga
//
LOCUS       DAZKHF020000695          713 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2201S31241
            SAMN43267800-rid25106413.denovo.699, whole genome shotgun sequence.
ACCESSION   DAZKHF020000695 DAZKHF020000000
VERSION     DAZKHF020000695.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267800
            Sequence Read Archive: SRR30307263
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 713)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 713)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 713)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350581.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350581.2
            Genome Coverage       :: 151x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:21:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,652
            CDSs (total)                      :: 5,557
            Genes (coding)                    :: 5,320
            CDSs (with protein)               :: 5,320
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 237
            CDSs (without protein)            :: 237
            Pseudo Genes (ambiguous residues) :: 0 of 237
            Pseudo Genes (frameshifted)       :: 102 of 237
            Pseudo Genes (incomplete)         :: 130 of 237
            Pseudo Genes (internal stop)      :: 52 of 237
            Pseudo Genes (multiple problems)  :: 41 of 237
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..713
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267800-rid25106413.denovo.699"
                     /serotype="O 26:H11"
                     /isolate="2201S31241"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-01"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            <1..>713
                     /locus_tag="ACHOSC_RS26920"
                     /old_locus_tag="pgaptmp_005384"
     CDS             <1..>713
                     /locus_tag="ACHOSC_RS26920"
                     /old_locus_tag="pgaptmp_005384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="RHS repeat-associated core domain-containing
                     protein"
                     /protein_id="HGU4551097.1"
                     /translation="VSEESRRWLASCGLTVEQMQNQMDPVYTPARKIHLYHCDHRGLP
                     LALISTEGATAWCAEYDEWGNLLNEENPHQLQQLIRLPGQQYDEESGLYYNRHRYYDP
                     LQGRYITQDPIGLKGGWNFYQYPLNPISNIDPLGLETLKCIKPLHSMGGTGERSGPDI
                     WGNPFYHQYLCVPDGKGDYTCGGQDQRGESKGDGLWGPGKASNDTKEAAGRCDLVETD
                     NSCVENCLKGKFKEVRPRY"
BASE COUNT          196 a          155 c          215 g          147 t
ORIGIN      
        1 gggtgagtga ggaaagccgc cgctggctgg catcgtgcgg cctgaccgtg gagcagatgc
       61 aaaaccagat ggacccggtg tacacgccgg cgcgaaaaat ccacctgtac cactgcgacc
      121 atcgcggcct gccgctggcg ctcatcagca cggaaggggc aacagcgtgg tgcgcagaat
      181 atgatgaatg gggcaacctg ctgaatgaag agaacccgca tcagctgcag cagcttatcc
      241 ggctgccggg gcagcagtat gatgaggagt ccggcctgta ttacaaccgc caccgctatt
      301 atgacccgct gcaggggcgg tatatcactc aggatccgat tgggctgaag gggggatgga
      361 atttttatca gtatccgttg aatccgatct caaatataga tccattagga ttagaaacac
      421 taaaatgcat taagccactg cattcaatgg gcggaactgg tgaaagaagc ggtccagata
      481 tatgggggaa tccgttctat catcaatatc tttgtgtccc agatggtaaa ggggactata
      541 cttgtggtgg ccaagaccaa cggggagaat caaaaggaga tggtctatgg gggccaggta
      601 aagcaagtaa tgatacaaaa gaagctgctg gccgttgtga cctcgttgaa accgataata
      661 gttgtgtgga gaactgttta aaagggaagt ttaaagaggt aaggccgcgt tat
//
LOCUS       DAZKHF020000696          711 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2201S31241
            SAMN43267800-rid25106413.denovo.700, whole genome shotgun sequence.
ACCESSION   DAZKHF020000696 DAZKHF020000000
VERSION     DAZKHF020000696.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267800
            Sequence Read Archive: SRR30307263
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 711)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 711)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 711)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350581.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350581.2
            Genome Coverage       :: 151x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:21:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,652
            CDSs (total)                      :: 5,557
            Genes (coding)                    :: 5,320
            CDSs (with protein)               :: 5,320
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 237
            CDSs (without protein)            :: 237
            Pseudo Genes (ambiguous residues) :: 0 of 237
            Pseudo Genes (frameshifted)       :: 102 of 237
            Pseudo Genes (incomplete)         :: 130 of 237
            Pseudo Genes (internal stop)      :: 52 of 237
            Pseudo Genes (multiple problems)  :: 41 of 237
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..711
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267800-rid25106413.denovo.700"
                     /serotype="O 26:H11"
                     /isolate="2201S31241"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-01"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            <1..>711
                     /locus_tag="ACHOSC_RS26925"
                     /old_locus_tag="pgaptmp_005385"
     CDS             <1..>711
                     /locus_tag="ACHOSC_RS26925"
                     /old_locus_tag="pgaptmp_005385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001443751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4551098.1"
                     /translation="VISNPKRLDGVPGQMNVVHIRFDSNSPVMADVQEEGKPQLWNHK
                     QNDALGLYLDLLIQAIDTDTINAEDWQKGDRLKSVALLIAYLDKANFYVMEDSGAWEE
                     DARLNTSSVALVTSGLERLSNLLSKKDSVFVSDLLREAKANELDEPLSTTRLNHLIDK
                     GYERITLQLDLGGESPGYLEKDKHYREADAALLNVIYPANLAKINTRRKEQVLKIVKK
                     LAGPYGIKRYEKDNYQSA"
BASE COUNT          237 a          138 c          158 g          178 t
ORIGIN      
        1 acgtcattag caaccccaaa cgactggatg gagttcccgg gcaaatgaat gttgtgcata
       61 tccgatttga cagcaactcc cccgtgatgg cagatgtgca ggaagaaggc aagccgcagc
      121 tgtggaacca taaacaaaat gatgcgttag gtctttatct tgatctcctt atacaagcaa
      181 tcgacactga tacaattaac gctgaagatt ggcaaaaagg tgaccgatta aaatcggttg
      241 cgctgctgat tgcttatctg gataaagcca atttctatgt tatggaagat tccggagcat
      301 gggaagaaga tgcccgatta aacacatctt ccgttgcact ggtgacctct ggactggaac
      361 gtttgtcgaa cttactctcg aagaaagact ctgtttttgt ctctgattta ctacgcgaag
      421 ctaaagccaa tgaactggat gaacctcttt caacaacgag gttgaaccac ctcatagata
      481 aaggttatga aagaataacg ttacagcttg atttaggggg agaatcccca ggttatctgg
      541 aaaaagataa acattacaga gaagccgatg cggctttact taatgttatt tatcctgcaa
      601 atcttgcaaa gataaacacc agaagaaaag agcaggttct aaaaatagtt aaaaaactag
      661 caggtccgta tggtataaaa agatacgaga aagataatta tcagtcggca a
//
LOCUS       DAZKHF020000697          710 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2201S31241
            SAMN43267800-rid25106413.denovo.701, whole genome shotgun sequence.
ACCESSION   DAZKHF020000697 DAZKHF020000000
VERSION     DAZKHF020000697.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267800
            Sequence Read Archive: SRR30307263
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 710)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 710)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 710)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350581.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350581.2
            Genome Coverage       :: 151x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:21:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,652
            CDSs (total)                      :: 5,557
            Genes (coding)                    :: 5,320
            CDSs (with protein)               :: 5,320
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 237
            CDSs (without protein)            :: 237
            Pseudo Genes (ambiguous residues) :: 0 of 237
            Pseudo Genes (frameshifted)       :: 102 of 237
            Pseudo Genes (incomplete)         :: 130 of 237
            Pseudo Genes (internal stop)      :: 52 of 237
            Pseudo Genes (multiple problems)  :: 41 of 237
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..710
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267800-rid25106413.denovo.701"
                     /serotype="O 26:H11"
                     /isolate="2201S31241"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-01"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            1..710
                     /locus_tag="ACHOSC_RS26930"
                     /old_locus_tag="pgaptmp_005386"
                     /pseudo
     CDS             1..710
                     /locus_tag="ACHOSC_RS26930"
                     /old_locus_tag="pgaptmp_005386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312403.1"
                     /note="internal stop; incomplete; missing N-terminus and
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT          270 a          143 c          110 g          187 t
ORIGIN      
        1 ttgactttac cccaggtata ataaatacat atcacggcga tatttataac tgcacaacaa
       61 ataccgataa tgctaaaacg ccagacacac cgaagtggcc ttgcgataat tgggaagaac
      121 aataacccat taattccaca ttctctggag aaggatatat ctctgatcaa tatgatttag
      181 cgcaacacca acttcaacaa attaatgcgt gccacacgaa cactacatat acaaatgcag
      241 actactcaaa agttgtggct caacttgtaa gtcttattac taacattgaa acgataagtt
      301 cgacacaact tacgcaacag acacaatcaa tacttaatca gattaataac attagatatg
      361 aaaaaaacaa aagtgctgaa tgtcgaatca ttgttgtcgc taatcctaaa cccgacaagg
      421 caatcataac taaaattagc gtagaagagg gaatacccat aacattcagt gtccaaacca
      481 tgttttcaga tactaatttt attgctgaac aacgagctga cttaccaact aatataaaag
      541 atattcaaag cctgtatcaa aaaatgacga agttatatat tgagcacagt gaaaataaaa
      601 acaggatgaa agtctttgcc ggaaccaact ttattgattt caatatgaca ggacagaacc
      661 tgtccgggtt cgttctaacc ttgtcaaggt tttatttcga agatctactt
//
LOCUS       DAZKHF020000698          709 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2201S31241
            SAMN43267800-rid25106413.denovo.702, whole genome shotgun sequence.
ACCESSION   DAZKHF020000698 DAZKHF020000000
VERSION     DAZKHF020000698.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267800
            Sequence Read Archive: SRR30307263
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 709)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 709)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 709)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350581.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350581.2
            Genome Coverage       :: 151x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:21:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,652
            CDSs (total)                      :: 5,557
            Genes (coding)                    :: 5,320
            CDSs (with protein)               :: 5,320
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 237
            CDSs (without protein)            :: 237
            Pseudo Genes (ambiguous residues) :: 0 of 237
            Pseudo Genes (frameshifted)       :: 102 of 237
            Pseudo Genes (incomplete)         :: 130 of 237
            Pseudo Genes (internal stop)      :: 52 of 237
            Pseudo Genes (multiple problems)  :: 41 of 237
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..709
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267800-rid25106413.denovo.702"
                     /serotype="O 26:H11"
                     /isolate="2201S31241"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-01"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            complement(<1..>709)
                     /locus_tag="ACHOSC_RS26935"
                     /old_locus_tag="pgaptmp_005387"
     CDS             complement(<1..>709)
                     /locus_tag="ACHOSC_RS26935"
                     /old_locus_tag="pgaptmp_005387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="RHS repeat protein"
                     /protein_id="HGU4551099.1"
                     /translation="YNEQGLANRVTPDSLPPVEWLTYGSGYLAGMKLGGTPLVEYTRD
                     RLHRETVRSFGSRAGSNAAYELTSTYTPAGQLQSQHLNSLVYDRDYGWNDNGDLVRIS
                     GPRQTREYGYSATGRLESVRTLAPGLDIRIPYATDPAGNRLPDPELHPDSTLTAWPDN
                     RIAEDAHYVYHYDEYGRLTEKTDRIPTGVIRTDDERTHHYHYDSQHRLVFHTRIQHGE
                     PLVESRYLYDPLGRRMA"
BASE COUNT           89 a          231 c          230 g          159 t
ORIGIN      
        1 ttcgccattc gccgtcccag cgggtcgtag aggtagcggc tctcgaccag tggctcgcca
       61 tgctgtatcc gcgtgtggaa caccaggcgg tgctggctgt cgtagtggta gtggtgggtc
      121 cgctcgtcgt ccgtccgtat cacacccgtc gggatgcggt ccgtcttctc cgtcagtctg
      181 ccgtattcat catagtggta aacatagtgc gcatcctctg cgatgcggtt atccggccac
      241 gccgtgagcg tgctgtccgg gtgtagctcc gggtccggca ggcggtttcc cgccgggtcg
      301 gtggcatacg ggatgcggat gtccaggccc ggtgcgaggg tgcgcacgct ctccagcctg
      361 cccgtggcac tgtagccgta ttcccgcgtc tgtcgcgggc cgctgatgcg caccaggtcg
      421 ccgttgtcat tccacccgta gtcacggtca tacaccaggc tgttcaggtg ctggctctgt
      481 aactggcctg cgggggtgta tgtgctggtc agttcatatg cggcattact gcctgccctg
      541 ctgccgaagc tgcgcaccgt ctcacggtgc agcctgtccc gcgtatactc gaccagcggc
      601 gtcccgccca gcttcatgcc cgccaggtaa ccgctgccgt aggtcagcca ctccaccggc
      661 ggcaggctgt ccggcgtgac gcggtttgcc agcccctgtt cgttgtagg
//
LOCUS       DAZKHF020000699          703 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2201S31241
            SAMN43267800-rid25106413.denovo.703, whole genome shotgun sequence.
ACCESSION   DAZKHF020000699 DAZKHF020000000
VERSION     DAZKHF020000699.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267800
            Sequence Read Archive: SRR30307263
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 703)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 703)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 703)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350581.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350581.2
            Genome Coverage       :: 151x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:21:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,652
            CDSs (total)                      :: 5,557
            Genes (coding)                    :: 5,320
            CDSs (with protein)               :: 5,320
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 237
            CDSs (without protein)            :: 237
            Pseudo Genes (ambiguous residues) :: 0 of 237
            Pseudo Genes (frameshifted)       :: 102 of 237
            Pseudo Genes (incomplete)         :: 130 of 237
            Pseudo Genes (internal stop)      :: 52 of 237
            Pseudo Genes (multiple problems)  :: 41 of 237
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..703
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267800-rid25106413.denovo.703"
                     /serotype="O 26:H11"
                     /isolate="2201S31241"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-01"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            <1..494
                     /locus_tag="ACHOSC_RS26940"
                     /old_locus_tag="pgaptmp_005388"
     CDS             <1..494
                     /locus_tag="ACHOSC_RS26940"
                     /old_locus_tag="pgaptmp_005388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="terminase small subunit"
                     /protein_id="HGU4551100.1"
                     /translation="AAGYSAKTARVKGSQLAKDPEVLAFIARKQCETVEVDEVPVYRQ
                     KKSEQEDKPRRREAAAIPQPDENNPEMPPSAVMSPGIEYMEDGLPDPVKAMGRLLVEN
                     INTDPRLALDAAYKLAQFTHHKKGDAGKKSAKGDAAKKAANRFAVPPPPRLVVNNDNE
                     GNG"
     gene            491..>703
                     /locus_tag="ACHOSC_RS26945"
                     /old_locus_tag="pgaptmp_005389"
     CDS             491..>703
                     /locus_tag="ACHOSC_RS26945"
                     /old_locus_tag="pgaptmp_005389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4551101.1"
                     /translation="MIPVWSTACPDWAERLKKGLSIIPAPIYPDQAAHALAIFKQLRI
                     VDAPGSPTFGESCAPWVFDLVAALFGS"
BASE COUNT          166 a          172 c          223 g          142 t
ORIGIN      
        1 ttgccgctgg ttattcggcg aagaccgcca gggttaaagg ctcgcagctg gcaaaagatc
       61 cggaggtgct tgcgtttata gcccgtaaac aatgcgagac ggtggaggtg gatgaggttc
      121 ctgtttaccg gcagaaaaaa tcagagcagg aggataaacc ccgtcgccgt gaggcggctg
      181 caataccaca gccggacgaa aacaatccgg agatgccacc gtccgcggtg atgtctcctg
      241 gtattgaata tatggaggat ggtcttcccg atccggtgaa agcgatgggg cgtcttctgg
      301 tggagaacat taataccgac cccaggctgg cgctggatgc ggcttataag ctggcgcagt
      361 tcacgcacca taaaaaaggg gatgccggta aaaaatcggc aaaaggtgac gcggcgaaaa
      421 aagcggctaa ccgttttgcg gtgccaccac caccccgcct ggtggtgaat aatgataatg
      481 agggcaacgg atgatacctg tgtggagcac ggcctgcccg gactgggcag agcgcctgaa
      541 aaaggggctg tcgattattc cggctccgat ttatccggac caggctgcac atgcactggc
      601 gatttttaaa caactgcgaa ttgtggatgc accgggtagc ccgacattcg gggagtcctg
      661 tgcaccgtgg gtgtttgacc tggtggcggc cctgtttggc tcc
//
LOCUS       DAZKHF020000700          699 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2201S31241
            SAMN43267800-rid25106413.denovo.704, whole genome shotgun sequence.
ACCESSION   DAZKHF020000700 DAZKHF020000000
VERSION     DAZKHF020000700.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267800
            Sequence Read Archive: SRR30307263
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 699)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 699)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 699)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350581.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350581.2
            Genome Coverage       :: 151x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:21:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,652
            CDSs (total)                      :: 5,557
            Genes (coding)                    :: 5,320
            CDSs (with protein)               :: 5,320
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 237
            CDSs (without protein)            :: 237
            Pseudo Genes (ambiguous residues) :: 0 of 237
            Pseudo Genes (frameshifted)       :: 102 of 237
            Pseudo Genes (incomplete)         :: 130 of 237
            Pseudo Genes (internal stop)      :: 52 of 237
            Pseudo Genes (multiple problems)  :: 41 of 237
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..699
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267800-rid25106413.denovo.704"
                     /serotype="O 26:H11"
                     /isolate="2201S31241"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-01"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            complement(312..>699)
                     /locus_tag="ACHOSC_RS26950"
                     /old_locus_tag="pgaptmp_005390"
     CDS             complement(312..>699)
                     /locus_tag="ACHOSC_RS26950"
                     /old_locus_tag="pgaptmp_005390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HGU4551102.1"
                     /translation="PLTDISYVRQMESWMITTRPRRREPLWAVTDETMRNWLKQAVRR
                     AEADGVHFSIPVTPHTFRHSYIMHMLYHRQPRKVIQALAGHRDPRSMEVYTRVFALDM
                     AATLVVPFTGDGRDAAEILRTLPPLK"
BASE COUNT          144 a          199 c          182 g          174 t
ORIGIN      
        1 cgtcatagca gactggccgg tcaaccgggt cagcgaactg cttccgtggc gcatagcact
       61 gccagctgaa taacacatcc ccgtcaatac ggccctcgct gtacgcttac gtttatattc
      121 agtttcctgc agtgctgcct gtggctgagc tgccatctgc ctgtccctta cgtgagtcac
      181 cccgtaacct gatgctgagg cattgctccc ttcataaaac atggtttact cactacagct
      241 tatctacctg ctccagctta tgttatgtct gttctgctga ccactgctta tataaggaag
      301 agcgtatgct gtcacttcag gggaggcagt gtacgcagga tctctgcagc atcgcgcccg
      361 tcacctgtga aaggcactac cagcgtggca gccatatcca gcgcaaaaac tctggtataa
      421 acctccatcg aacgtggatc cctgtgacca gccagtgcct ggatgacttt ccggggctgg
      481 cggtgataga gcatgtgcat gatatagctg tgccggaagg tgtgtggtgt gaccggaatc
      541 gaaaagtgta ccccgtcagc ttcggcccgt ctgacagcct gcttcagcca gttgcgcatg
      601 gtttcgtcag tcacggccca taatggttca cgacgacggg gccgggtggt gatcatccag
      661 ctttccatct gcctgacata gcttatatct gtcagcgga
//
LOCUS       DAZKHF020000701          697 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2201S31241
            SAMN43267800-rid25106413.denovo.705, whole genome shotgun sequence.
ACCESSION   DAZKHF020000701 DAZKHF020000000
VERSION     DAZKHF020000701.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267800
            Sequence Read Archive: SRR30307263
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 697)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 697)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 697)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350581.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350581.2
            Genome Coverage       :: 151x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:21:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,652
            CDSs (total)                      :: 5,557
            Genes (coding)                    :: 5,320
            CDSs (with protein)               :: 5,320
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 237
            CDSs (without protein)            :: 237
            Pseudo Genes (ambiguous residues) :: 0 of 237
            Pseudo Genes (frameshifted)       :: 102 of 237
            Pseudo Genes (incomplete)         :: 130 of 237
            Pseudo Genes (internal stop)      :: 52 of 237
            Pseudo Genes (multiple problems)  :: 41 of 237
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..697
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267800-rid25106413.denovo.705"
                     /serotype="O 26:H11"
                     /isolate="2201S31241"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-01"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            complement(402..491)
                     /locus_tag="ACHOSC_RS26955"
                     /old_locus_tag="pgaptmp_005391"
                     /pseudo
     CDS             complement(402..491)
                     /locus_tag="ACHOSC_RS26955"
                     /old_locus_tag="pgaptmp_005391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308358.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT          203 a          120 c          155 g          219 t
ORIGIN      
        1 attttttgac atgtatcaca aatttgaata gatgtattac atcagctatc ttttattgca
       61 ccaacgtcat tgatatatgt cgactgaagt cagttccggg aatgagtctg atctcaagac
      121 tggcccagtc cgggcgttga ttggcgctga ggagcatacc tcatttcatc ataatgtcgt
      181 atctcctggg gtgttataca agatatcgtt gttggtaacc tgggaaagga attgagttct
      241 attaaaccgt caactatgcc ggatacatac tggattacac tgcaggcacg ccttatgaga
      301 gaacgtgccg cagtgacggg ttaattatct gaaagaattt gtgaggctgt atcggttact
      361 cattgatttg atagttttac tctcgggaga ataatagata tttaatccat taacggaaac
      421 cagccagttc ctttcgatgc ctgaatttga tcccatagtt tatccggaat ggttaattca
      481 tgaacaagtt gtgttatcgt tcatgagaag cataacgtaa agggaaaagc tcgattagac
      541 ggcagaattt gtcaggggtt atgaacgaaa ttcataaatc tgtttgagtg ttgcgatggg
      601 tagtgcaagt tcgatatctc cgcaatttac agtccgatga aggaaaatga atatccataa
      661 aaaatatatt ggtttatcct ggcatatata cctattt
//
LOCUS       DAZKHF020000702          689 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2201S31241
            SAMN43267800-rid25106413.denovo.706, whole genome shotgun sequence.
ACCESSION   DAZKHF020000702 DAZKHF020000000
VERSION     DAZKHF020000702.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267800
            Sequence Read Archive: SRR30307263
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 689)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 689)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 689)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350581.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350581.2
            Genome Coverage       :: 151x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:21:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,652
            CDSs (total)                      :: 5,557
            Genes (coding)                    :: 5,320
            CDSs (with protein)               :: 5,320
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 237
            CDSs (without protein)            :: 237
            Pseudo Genes (ambiguous residues) :: 0 of 237
            Pseudo Genes (frameshifted)       :: 102 of 237
            Pseudo Genes (incomplete)         :: 130 of 237
            Pseudo Genes (internal stop)      :: 52 of 237
            Pseudo Genes (multiple problems)  :: 41 of 237
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..689
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267800-rid25106413.denovo.706"
                     /serotype="O 26:H11"
                     /isolate="2201S31241"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-01"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            <1..612
                     /locus_tag="ACHOSC_RS26960"
                     /old_locus_tag="pgaptmp_005392"
     CDS             <1..612
                     /locus_tag="ACHOSC_RS26960"
                     /old_locus_tag="pgaptmp_005392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000015210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polymorphic toxin type 34 domain-containing
                     protein"
                     /protein_id="HGU4551103.1"
                     /translation="NQLNEENPHQLQQLIRLPGQQYDEESGLYYNRHRYYDPLRGRYI
                     TQDPIGLKGGWNFYQYPLNPVTNTDPLGLEVFPRPFPLPIPWPKSPAQQQADDNAAKA
                     LTKWWNDTASQRIFDSLILNNPGLALDITMIASRGNVADTGITDRVNDIINDRFWSDG
                     KKTDRCDVLQELIDCGDISAKDAKSTQKAWNCRHSRQSNDKKR"
BASE COUNT          229 a          140 c          162 g          158 t
ORIGIN      
        1 aaccagctga atgaagagaa cccgcaccag ctgcagcagc tcatccgcct gccggggcag
       61 cagtatgatg aggagtccgg cctgtattat aaccgccacc gctattatga cccgctgcgg
      121 gggaggtata tcactcagga tccgattggg ctgaaggggg gatggaattt ttatcagtat
      181 ccgttgaatc cagttacgaa tacagatcct ctggggttag aagtttttcc tagaccattc
      241 cccttgccaa ttccatggcc caaaagccct gcacagcagc aagcagatga taatgctgca
      301 aaagcattga caaaatggtg gaacgataca gcatcacaaa gaatatttga ctctctaata
      361 ttgaataatc cgggactagc attagatata acaatgatag cttctcgtgg aaatgttgca
      421 gacacaggga taactgatcg tgtcaatgac ataataaatg acagattctg gagtgatggg
      481 aaaaaaaccg acagatgtga cgtacttcag gaactaattg attgtggtga tattagtgct
      541 aaagatgcaa aaagcacaca gaaagcctgg aattgtcgtc actccagaca gtcaaacgat
      601 aaaaaaagat agcccttgtg gaggttcctg caatgtcaaa tacataccag aaaagaaagg
      661 caagtaaaga gtatggttta tataatcaa
//
LOCUS       DAZKHF020000703          689 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2201S31241
            SAMN43267800-rid25106413.denovo.707, whole genome shotgun sequence.
ACCESSION   DAZKHF020000703 DAZKHF020000000
VERSION     DAZKHF020000703.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267800
            Sequence Read Archive: SRR30307263
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 689)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 689)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 689)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350581.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350581.2
            Genome Coverage       :: 151x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:21:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,652
            CDSs (total)                      :: 5,557
            Genes (coding)                    :: 5,320
            CDSs (with protein)               :: 5,320
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 237
            CDSs (without protein)            :: 237
            Pseudo Genes (ambiguous residues) :: 0 of 237
            Pseudo Genes (frameshifted)       :: 102 of 237
            Pseudo Genes (incomplete)         :: 130 of 237
            Pseudo Genes (internal stop)      :: 52 of 237
            Pseudo Genes (multiple problems)  :: 41 of 237
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..689
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267800-rid25106413.denovo.707"
                     /serotype="O 26:H11"
                     /isolate="2201S31241"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-01"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            19..>689
                     /locus_tag="ACHOSC_RS26965"
                     /old_locus_tag="pgaptmp_005393"
     CDS             19..>689
                     /locus_tag="ACHOSC_RS26965"
                     /old_locus_tag="pgaptmp_005393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HGU4551104.1"
                     /translation="MGRKWANIVAKKTAKDGATSKIYAKFGVEIYAAAKQGEPDPELN
                     TSLKFVIERAKQAQVPKHVIDKAIDKAKGGGDETFVQGRYEGFGPNGSMIIAETLTSN
                     VNRTIANVRTIFNKKGGNIGAAGSVSYMFDNTGVIVFKGTDPDHIFEILLEAEVDVRD
                     VTEEEGNIVIYTEPTDLHKGIAALKAAGITEFSTTELEMIAQSEVELSPEDLEIFEGL
                     VDALE"
BASE COUNT          225 a          119 c          156 g          189 t
ORIGIN      
        1 ttcgaggaga tcattccagt gggacgtaaa tgggccaata ttgttgctaa aaaaacggct
       61 aaagacggtg caacgtctaa aatttatgca aaattcggtg tagaaatcta tgctgctgcc
      121 aaacaaggtg aacccgatcc agaattaaac acatctttaa aattcgttat tgaacgtgca
      181 aagcaggcac aagttccaaa gcacgttatt gataaagcaa ttgataaagc caaaggcggc
      241 ggagatgaaa cgttcgtaca gggacgttat gaaggctttg gtcctaatgg ctcaatgatt
      301 attgctgaga cattgacttc aaatgttaac cgtacgattg ctaacgttcg cacaattttc
      361 aataaaaaag gcggcaatat cggagcggca ggttctgtca gctatatgtt tgacaatacg
      421 ggtgtgattg tatttaaagg aacagaccct gaccatattt ttgaaatttt gcttgaagct
      481 gaagttgatg ttcgtgatgt aactgaagaa gaaggtaata ttgttattta tactgaacct
      541 actgaccttc ataaaggaat cgcggcgcta aaagcagctg gaatcactga gttctcaaca
      601 acagaattag aaatgattgc tcaatctgaa gttgagcttt ccccagaaga tttagaaatc
      661 tttgaagggc ttgttgatgc ccttgaaga
//
LOCUS       DAZKHF020000704          686 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2201S31241
            SAMN43267800-rid25106413.denovo.708, whole genome shotgun sequence.
ACCESSION   DAZKHF020000704 DAZKHF020000000
VERSION     DAZKHF020000704.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267800
            Sequence Read Archive: SRR30307263
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 686)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 686)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 686)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350581.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350581.2
            Genome Coverage       :: 151x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:21:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,652
            CDSs (total)                      :: 5,557
            Genes (coding)                    :: 5,320
            CDSs (with protein)               :: 5,320
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 237
            CDSs (without protein)            :: 237
            Pseudo Genes (ambiguous residues) :: 0 of 237
            Pseudo Genes (frameshifted)       :: 102 of 237
            Pseudo Genes (incomplete)         :: 130 of 237
            Pseudo Genes (internal stop)      :: 52 of 237
            Pseudo Genes (multiple problems)  :: 41 of 237
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..686
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267800-rid25106413.denovo.708"
                     /serotype="O 26:H11"
                     /isolate="2201S31241"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-01"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            61..465
                     /locus_tag="ACHOSC_RS26970"
                     /old_locus_tag="pgaptmp_005394"
     CDS             61..465
                     /locus_tag="ACHOSC_RS26970"
                     /old_locus_tag="pgaptmp_005394"
                     /inference="COORDINATES: protein motif:HMM:NF013677.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HGU4551105.1"
                     /translation="MKSLTAVRKKSPNYPVEFKIKMVELSHRPEISVAQLAREHGIND
                     NLLFKWRQYWREGKLRPPSTTENNVPELLPITLDAEDVVPTTSPRSQPVAAATPESLN
                     ISCEVTFRHGSLRLNGAISENILNLLIRELKR"
     gene            462..>686
                     /gene="tnpB"
                     /locus_tag="ACHOSC_RS26975"
                     /old_locus_tag="pgaptmp_005395"
     CDS             462..>686
                     /gene="tnpB"
                     /locus_tag="ACHOSC_RS26975"
                     /old_locus_tag="pgaptmp_005395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001536669.1"
                     /note="TnpB, as the term is used for proteins encoded by
                     IS66 family insertion elements, is considered an accessory
                     protein, since TnpC, encoded by a neighboring gene, is a
                     DDE family transposase; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family insertion sequence element accessory
                     protein TnpB"
                     /protein_id="HGU4551106.1"
                     /translation="MIPLPSGTKIWLVAGITDMRNGFNGLAAKVQTTLKDDPMSGHVF
                     IFRGRNGSQVKLLWSTGDGLCLLTKRLERGR"
BASE COUNT          183 a          184 c          170 g          149 t
ORIGIN      
        1 tctggcagat cctgaaattc ctgagagaat agtggacacc aaatatggtg gacgctatcc
       61 atgaaatcat taaccgcagt gcgtaaaaaa agccctaatt atcccgttga gttcaaaatc
      121 aaaatggttg aactctcgca tcgaccagag atctccgtag cgcaactcgc tcgtgagcat
      181 gggatcaacg ataatttgct gttcaagtgg cgccagtact ggcgcgaagg aaaactacgt
      241 cctccttcaa caacagaaaa caacgtgcct gagctgctcc cgataacact tgatgccgaa
      301 gatgttgtcc ctacaacctc cccccggtca caacctgtag ctgctgcgac acctgaatca
      361 ctcaatatca gctgtgaagt gacgttccgg cacggatcac tccgtctgaa tggtgccatc
      421 agcgaaaata tcctgaacct gctgatacgg gagctcaaac gttgatccca ttaccatcag
      481 ggacaaagat ctggctggtc gctggcatca ccgatatgag aaacggcttc aacggcctgg
      541 cggcaaaggt gcagacgacg ctgaaagacg atccgatgtc aggtcacgtt tttatcttcc
      601 gtgggcgtaa tggcagtcag gtaaagctcc tctggtctac cggcgatgga ctgtgtctgc
      661 tgaccaaacg gctggagcgc ggccgc
//
LOCUS       DAZKHF020000705          680 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2201S31241
            SAMN43267800-rid25106413.denovo.709, whole genome shotgun sequence.
ACCESSION   DAZKHF020000705 DAZKHF020000000
VERSION     DAZKHF020000705.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267800
            Sequence Read Archive: SRR30307263
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 680)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 680)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 680)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350581.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350581.2
            Genome Coverage       :: 151x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:21:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,652
            CDSs (total)                      :: 5,557
            Genes (coding)                    :: 5,320
            CDSs (with protein)               :: 5,320
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 237
            CDSs (without protein)            :: 237
            Pseudo Genes (ambiguous residues) :: 0 of 237
            Pseudo Genes (frameshifted)       :: 102 of 237
            Pseudo Genes (incomplete)         :: 130 of 237
            Pseudo Genes (internal stop)      :: 52 of 237
            Pseudo Genes (multiple problems)  :: 41 of 237
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..680
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267800-rid25106413.denovo.709"
                     /serotype="O 26:H11"
                     /isolate="2201S31241"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-01"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
BASE COUNT          254 a          160 c           98 g          168 t
ORIGIN      
        1 aaatacttct gtataatcgc cagccaatga tgagaatgga ttaaaataac acttcttcga
       61 caaacacaaa aacagccttc attttacatc atcatccagt ttcagttaac cccggacgag
      121 cgacagtgtt tcagtacagg aattaaaagc cagcttttgc tcagtaaaac acactcaact
      181 aataatatct cagcagatat tatcctgacc aggacacctc acattactga attctcttcc
      241 gacagtatca ctgcacaaat aaaaaagaac gggataaata ttcagaccgg aagaatggtg
      301 tcacaaaagc atttaatcat tacgcatgac caggaaggga aacacttaac cacatggtat
      361 caacagatat cattacgtgt gcactcttat aaacaacaca cagaagaagt aagataatta
      421 taaacgtaaa tttaatatct cccactgaca tctcccccac aatacactga aaaatactca
      481 cagcagtaaa cacagacaca cctgataatc atgcagccga taaatatttt cccctttacg
      541 gagaaatcag cctgctgttt aacaataacg gcgtattttc atggaaggag atcacccagt
      601 aacggataag tcctgatctc cttcatgtta atacgcaaca atcccatcga ttcaaaacaa
      661 tttcagacag atattatctc
//
LOCUS       DAZKHF020000706          679 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2201S31241
            SAMN43267800-rid25106413.denovo.710, whole genome shotgun sequence.
ACCESSION   DAZKHF020000706 DAZKHF020000000
VERSION     DAZKHF020000706.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267800
            Sequence Read Archive: SRR30307263
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 679)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 679)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 679)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350581.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350581.2
            Genome Coverage       :: 151x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:21:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,652
            CDSs (total)                      :: 5,557
            Genes (coding)                    :: 5,320
            CDSs (with protein)               :: 5,320
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 237
            CDSs (without protein)            :: 237
            Pseudo Genes (ambiguous residues) :: 0 of 237
            Pseudo Genes (frameshifted)       :: 102 of 237
            Pseudo Genes (incomplete)         :: 130 of 237
            Pseudo Genes (internal stop)      :: 52 of 237
            Pseudo Genes (multiple problems)  :: 41 of 237
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..679
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267800-rid25106413.denovo.710"
                     /serotype="O 26:H11"
                     /isolate="2201S31241"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-01"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            complement(180..656)
                     /locus_tag="ACHOSC_RS26980"
                     /old_locus_tag="pgaptmp_005396"
     CDS             complement(180..656)
                     /locus_tag="ACHOSC_RS26980"
                     /old_locus_tag="pgaptmp_005396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="secretion protein EspH"
                     /protein_id="HGU4551107.1"
                     /translation="MTSHHMSWGKFSRTLHVLNEDIVDEIQLKARTQQRHHRVYSEMA
                     DYSVVSFRGKEYSVRFIKDYKTDNYVYKVQKMTQESGCLSRLFSFLSGGVTKALESKL
                     NERHITPLSPTWFPRTPLEGVLTERGLSSLLRRVQSTENLDYSNAMISNSFSNSVV"
BASE COUNT          217 a          147 c          117 g          198 t
ORIGIN      
        1 atccattcag ctcaatcccc tcctcatcac agaatctctg acttctgcca gccaaactca
       61 ctctgaatat tcatcccata ttttcctaaa aacaatgtat tatcgacata aaacaaacag
      121 aaaaactcca gaatcacaat tattctggag ttaaaccagg aggaaagctc aaatgataat
      181 cacactacgg agttagagaa agagtttgat atcatagcat tactataatc aaggttttca
      241 gtagactgta cgcgacgtaa caaagaagat aatcctcttt ccgttaagac cccctccagt
      301 ggagttcgag gaaaccaggt tggagaaagt ggagtgatat gcctctcatt tagtttactt
      361 tccaaagcct tagtcacacc gccagaaaga aagctaaata atcttgagag acacccactt
      421 tcctgagtca tcttttgtac tttgtacaca taattatctg ttttgtaatc tttaataaaa
      481 cgaacagaat attctttgcc ccgaaaggag acaacggagt aatccgccat ttctgaatat
      541 actcgatggt ggcgttgttg tgttcgggct ttaagctgaa tttcatctac gatatcttca
      601 ttcaatacat gaagagttct gctaaacttg ccccatgaca tatggtgact tgtcagcgaa
      661 gtattgagta ttattcctg
//
LOCUS       DAZKHF020000707          678 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2201S31241
            SAMN43267800-rid25106413.denovo.711, whole genome shotgun sequence.
ACCESSION   DAZKHF020000707 DAZKHF020000000
VERSION     DAZKHF020000707.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267800
            Sequence Read Archive: SRR30307263
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 678)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 678)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 678)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350581.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350581.2
            Genome Coverage       :: 151x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:21:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,652
            CDSs (total)                      :: 5,557
            Genes (coding)                    :: 5,320
            CDSs (with protein)               :: 5,320
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 237
            CDSs (without protein)            :: 237
            Pseudo Genes (ambiguous residues) :: 0 of 237
            Pseudo Genes (frameshifted)       :: 102 of 237
            Pseudo Genes (incomplete)         :: 130 of 237
            Pseudo Genes (internal stop)      :: 52 of 237
            Pseudo Genes (multiple problems)  :: 41 of 237
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..678
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267800-rid25106413.denovo.711"
                     /serotype="O 26:H11"
                     /isolate="2201S31241"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-01"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            <1..>678
                     /locus_tag="ACHOSC_RS26985"
                     /old_locus_tag="pgaptmp_005397"
     CDS             <1..>678
                     /locus_tag="ACHOSC_RS26985"
                     /old_locus_tag="pgaptmp_005397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3491 domain-containing protein"
                     /protein_id="HGU4551108.1"
                     /translation="DMRENPPILHGEKSYNSGKNIGSHPDMTDREIMESGAYNFACTK
                     LSDAGEPDIFGWGDKDVCNSMELSESQLANGYSNRQWPSQSPVIPEGIYNTVYLGYGE
                     TLRANTEVTLSSTGYFYEIARAYTDDELSEPLLTVCNQRSSVIGGKEPLTIIIPALEH
                     GMLGSNLHMIERFKNYNFSISGGKGGIKLMVGGIGDYNIECTPGVRNIISFEQLSRDF
                     NLDLDLSD"
BASE COUNT          226 a          102 c          146 g          204 t
ORIGIN      
        1 gatatgagag aaaaccctcc gatattacat ggtgaaaaat catataatag cgggaagaat
       61 attggaagtc atccagatat gactgacaga gaaattatgg agtctggagc atacaatttt
      121 gcatgtacaa aactctcaga tgcaggtgaa cccgatattt tcggttgggg agataaggat
      181 gtatgtaatt caatggaact gtctgaatct caattagcta atgggtattc taaccgccaa
      241 tggccttcac aaagtccagt tattcctgaa gggatttata atacagtata tcttgggtat
      301 ggagaaacat tgcgagctaa tacagaagtt acattgagta gcactggtta cttttacgag
      361 attgcaaggg catatactga tgatgaacta tctgagccat tattaacagt atgtaaccaa
      421 cgtagtagtg taattggtgg aaaagaacca ttaacaataa tcattccagc tcttgaacat
      481 ggtatgcttg gctctaatct acatatgatt gaacgtttta aaaactacaa tttttcaata
      541 tcaggaggga agggcgggat aaaattaatg gttggtggta ttggtgatta caacatagaa
      601 tgtacccccg gtgttagaaa tattatttct tttgaacaat tatcacgtga cttcaatctg
      661 gatttggatt tatcagat
//
LOCUS       DAZKHF020000708          678 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2201S31241
            SAMN43267800-rid25106413.denovo.712, whole genome shotgun sequence.
ACCESSION   DAZKHF020000708 DAZKHF020000000
VERSION     DAZKHF020000708.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267800
            Sequence Read Archive: SRR30307263
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 678)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 678)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 678)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350581.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350581.2
            Genome Coverage       :: 151x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:21:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,652
            CDSs (total)                      :: 5,557
            Genes (coding)                    :: 5,320
            CDSs (with protein)               :: 5,320
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 237
            CDSs (without protein)            :: 237
            Pseudo Genes (ambiguous residues) :: 0 of 237
            Pseudo Genes (frameshifted)       :: 102 of 237
            Pseudo Genes (incomplete)         :: 130 of 237
            Pseudo Genes (internal stop)      :: 52 of 237
            Pseudo Genes (multiple problems)  :: 41 of 237
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..678
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267800-rid25106413.denovo.712"
                     /serotype="O 26:H11"
                     /isolate="2201S31241"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-01"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            <1..>678
                     /locus_tag="ACHOSC_RS26990"
                     /old_locus_tag="pgaptmp_005398"
     CDS             <1..>678
                     /locus_tag="ACHOSC_RS26990"
                     /old_locus_tag="pgaptmp_005398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="RHS element protein"
                     /protein_id="HGU4551109.1"
                     /translation="WGKAVRTTQGGLTRSMEYDAAGRVIRLTSENGSHTTFRYDVLDR
                     LIQETGFDGRTQRYHHDLTGKLIRSEDEGLVTHWHYDEADRLTHRTVKGETAERWRYD
                     ERGWLTDISHISEGHRVTVHYGYDEKGRLTGERQTVHHPQTEALLWQHETRHAYNAQG
                     LANRCIPDSLPAVEWLTYGSGWLSGMKLGDTPLVEYTRDRLHRETLRSFGRYELTTAY
                     TPAGQLQ"
BASE COUNT          156 a          211 c          214 g           97 t
ORIGIN      
        1 gtggggaaag gccgtccgta ccacgcaggg cgggctgacg cgcagtatgg aatacgatgc
       61 tgccggacgg gtcatccgcc tgaccagtga aaacggcagc cacaccacct tccgttacga
      121 tgtactcgac cggctgatac aggaaaccgg ctttgacggc cgcacacagc gttatcacca
      181 cgacctgacc ggcaaactta tccgcagcga ggatgagggg ctggtcaccc actggcacta
      241 tgacgaagca gaccgcctca cgcaccgcac cgtgaagggt gaaaccgcag agcggtggcg
      301 gtacgacgaa cgcggctggc tgacagacat cagccatatc agcgaagggc accgggtgac
      361 ggtgcattac gggtatgatg agaaaggccg cctgaccggt gagcgtcaga cggtgcatca
      421 cccgcagacg gaagcactgc tctggcagca tgagaccaga catgcgtaca acgcgcaggg
      481 gctggcgaac cgctgtatac cggacagcct gcccgccgtg gaatggctga cctacggcag
      541 cggctggctg tcaggcatga aactcggcga cacaccgctg gtggagtaca cccgcgaccg
      601 cctgcaccgg gaaacgctgc gcagcttcgg tcgttatgaa ctcaccaccg cttacacccc
      661 tgccgggcag ttacagag
//
LOCUS       DAZKHF020000709          677 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2201S31241
            SAMN43267800-rid25106413.denovo.713, whole genome shotgun sequence.
ACCESSION   DAZKHF020000709 DAZKHF020000000
VERSION     DAZKHF020000709.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267800
            Sequence Read Archive: SRR30307263
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 677)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 677)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 677)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350581.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350581.2
            Genome Coverage       :: 151x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:21:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,652
            CDSs (total)                      :: 5,557
            Genes (coding)                    :: 5,320
            CDSs (with protein)               :: 5,320
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 237
            CDSs (without protein)            :: 237
            Pseudo Genes (ambiguous residues) :: 0 of 237
            Pseudo Genes (frameshifted)       :: 102 of 237
            Pseudo Genes (incomplete)         :: 130 of 237
            Pseudo Genes (internal stop)      :: 52 of 237
            Pseudo Genes (multiple problems)  :: 41 of 237
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..677
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267800-rid25106413.denovo.713"
                     /serotype="O 26:H11"
                     /isolate="2201S31241"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-01"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            <1..>677
                     /locus_tag="ACHOSC_RS26995"
                     /old_locus_tag="pgaptmp_005399"
     CDS             <1..>677
                     /locus_tag="ACHOSC_RS26995"
                     /old_locus_tag="pgaptmp_005399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="YfdQ family protein"
                     /protein_id="HGU4551110.1"
                     /translation="SQNLDATAINQIHALISAQGVNEIISNIGADAVALPENFRIHDL
                     EKFNLNRFRFRGALSTASIDDFTRYSKDLADEGTRCFIDADNMRAVSVLNLGTIDEPG
                     HADNTATLKLKKTAPFSALLSVNGERNSQKSLAEWIEDWADYLVGFDANGDAIQATKA
                     AAAIRKITIEANQTADFEDNDFSGKRSLMESVEAKTKDIMPVAFEFKCVPFEGLKERP
                     FKLRLSI"
BASE COUNT          182 a          161 c          153 g          181 t
ORIGIN      
        1 tgtctcagaa cttagacgca accgcaatta atcaaatcca tgcccttatt tctgctcagg
       61 gtgttaatga aattatcagt aatattggtg ccgatgctgt ggcattgcct gagaatttcc
      121 gcattcatga tctggaaaaa tttaatttaa atcgcttccg tttccgtggt gcgctttcca
      181 ctgccagcat cgatgacttt acccgttatt ctaaagatct tgcagatgaa ggcacccgct
      241 gctttatcga tgccgataat atgcgtgccg tcagtgtgct taacctgggt actattgatg
      301 aaccaggtca cgcagataac accgccactc tcaaactgaa aaagacagca ccgttctctg
      361 ctctgttgtc tgttaatggc gagcgtaact cccaaaagtc actggcagaa tggattgaag
      421 actgggccga ctaccttgtg ggctttgatg ctaatggtga cgccattcag gcaaccaaag
      481 cggctgcggc gatccgtaaa atcacaattg aagcgaacca gaccgctgat tttgaagaca
      541 atgacttcag cggcaaacgc tccctgatgg agtctgtcga agcgaagacc aaagacatta
      601 tgccagtggc atttgaattt aaatgcgttc cgtttgaagg cctgaaagaa cgtccgttta
      661 aattacgcct cagcatt
//
LOCUS       DAZKHF020000710          671 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2201S31241
            SAMN43267800-rid25106413.denovo.714, whole genome shotgun sequence.
ACCESSION   DAZKHF020000710 DAZKHF020000000
VERSION     DAZKHF020000710.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267800
            Sequence Read Archive: SRR30307263
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 671)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 671)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 671)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350581.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350581.2
            Genome Coverage       :: 151x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:21:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,652
            CDSs (total)                      :: 5,557
            Genes (coding)                    :: 5,320
            CDSs (with protein)               :: 5,320
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 237
            CDSs (without protein)            :: 237
            Pseudo Genes (ambiguous residues) :: 0 of 237
            Pseudo Genes (frameshifted)       :: 102 of 237
            Pseudo Genes (incomplete)         :: 130 of 237
            Pseudo Genes (internal stop)      :: 52 of 237
            Pseudo Genes (multiple problems)  :: 41 of 237
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..671
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267800-rid25106413.denovo.714"
                     /serotype="O 26:H11"
                     /isolate="2201S31241"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-01"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            <1..>671
                     /locus_tag="ACHOSC_RS27000"
                     /old_locus_tag="pgaptmp_005400"
     CDS             <1..>671
                     /locus_tag="ACHOSC_RS27000"
                     /old_locus_tag="pgaptmp_005400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tape measure protein"
                     /protein_id="HGU4551111.1"
                     /translation="TAFSDNAALFARSAASMREYGYSADDVLKVTEAISTGLKISGAS
                     TAEAGSVITQFSQALAQGVLRGEEFNSVNESGDRIIRALAAGMGVARKDLKAMADDGQ
                     LTADKVVPALISQLGVLRDEYAAMPETVSGSITKVENAFMAWVGGVNEASGVTKTLSG
                     VLNGVAGQIDNVATAVGALVAVGVARYFGNMASGAMSATAGLVTAARNEVALAEAQFR
                     GTQIAT"
BASE COUNT          136 a          152 c          233 g          150 t
ORIGIN      
        1 accgcttttt cggataatgc ggccctgttt gcccgttcgg ctgcctcgat gcgtgaatat
       61 ggttacagtg ctgatgatgt gctgaaggtg acggaggcca tttccacggg gctgaaaatc
      121 tccggtgcca gtacggctga ggcgggttcg gtgatcaccc agttcagcca ggcgctggca
      181 cagggtgtgt tgcgcggtga ggaatttaat tcggtcaatg aaagtggtga ccggatcatt
      241 cgtgcactgg ctgcaggcat gggcgtggcc cgtaaagatc tgaaggcgat ggcggacgat
      301 ggtcaactga cggcggataa agtcgttcct gcgttaatca gccagctggg ggtattgcgt
      361 gatgaatatg ccgccatgcc agaaacggtt tccggtagta tcacgaaggt ggaaaacgcc
      421 tttatggcct gggtgggcgg agtgaatgag gccagcgggg tgacaaaaac gctctccggc
      481 gtgctgaacg gtgttgccgg acagattgat aatgtggcaa cagctgtggg tgcgctggtt
      541 gccgtcgggg ttgcccggta ctttggcaat atggcctccg gagcgatgtc tgccacggca
      601 ggacttgtga cggctgcacg taatgaagtt gcactggcgg aagcacagtt caggggaacg
      661 cagattgcca c
//
LOCUS       DAZKHF020000711          671 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2201S31241
            SAMN43267800-rid25106413.denovo.715, whole genome shotgun sequence.
ACCESSION   DAZKHF020000711 DAZKHF020000000
VERSION     DAZKHF020000711.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267800
            Sequence Read Archive: SRR30307263
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 671)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 671)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 671)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350581.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350581.2
            Genome Coverage       :: 151x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:21:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,652
            CDSs (total)                      :: 5,557
            Genes (coding)                    :: 5,320
            CDSs (with protein)               :: 5,320
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 237
            CDSs (without protein)            :: 237
            Pseudo Genes (ambiguous residues) :: 0 of 237
            Pseudo Genes (frameshifted)       :: 102 of 237
            Pseudo Genes (incomplete)         :: 130 of 237
            Pseudo Genes (internal stop)      :: 52 of 237
            Pseudo Genes (multiple problems)  :: 41 of 237
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..671
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267800-rid25106413.denovo.715"
                     /serotype="O 26:H11"
                     /isolate="2201S31241"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-01"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            <1..612
                     /locus_tag="ACHOSC_RS27005"
                     /old_locus_tag="pgaptmp_005401"
     CDS             <1..612
                     /locus_tag="ACHOSC_RS27005"
                     /old_locus_tag="pgaptmp_005401"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0046677 - response to antibiotic [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CatB-related O-acetyltransferase"
                     /protein_id="HGU4551112.1"
                     /translation="EYSTMPVKNFSSVGAFSFPTCHFSGNIRIGRFCSIASNVKIMGG
                     NHPLNRFTTHMMTYNGEFDKFAMSEFERSWTLKPFITKPENPIIGNDVWIGNDVVLKG
                     GIAIGDGAVIAANSVVTKDVPPYAIVAGVPAKIIRFRFDSNVIDELLRIKWWNYNYSD
                     LPDNNKCDDINYFVEEMNRLISDGNIQERDYKKFNLSEVFRGL"
BASE COUNT          214 a           96 c          144 g          217 t
ORIGIN      
        1 gaatattcaa caatgcctgt gaaaaatttc tccagtgtcg gcgctttttc atttccgacc
       61 tgccattttt cagggaatat acgtataggt cgattttgta gtattgcatc aaatgtaaaa
      121 ataatgggtg gaaatcatcc tttgaatcgt tttactactc acatgatgac ttataacggt
      181 gaatttgata agtttgctat gagtgaattt gaacgtagct ggacattgaa accattcatt
      241 accaaacctg aaaatccgat tataggaaat gatgtctgga taggaaatga tgttgtattg
      301 aaaggtggca tcgcaatagg tgatggtgct gttattgctg ccaattccgt tgttaccaaa
      361 gatgtgcccc catatgccat agttgcagga gttcctgcaa aaataatcag atttaggttt
      421 gattcaaatg ttattgatga actgttgaga ataaaatggt ggaattataa ctactcagat
      481 cttccggata ataacaagtg tgatgatata aattattttg ttgaagagat gaatcgattg
      541 atatcagatg gaaatattca agagagggac tataaaaaat tcaatctttc tgaggtattc
      601 agaggcctat aattatctgt tttgcatgat aaatgggcaa gataaccagg gcggggtttg
      661 caatttcaat g
//
LOCUS       DAZKHF020000712          665 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2201S31241
            SAMN43267800-rid25106413.denovo.716, whole genome shotgun sequence.
ACCESSION   DAZKHF020000712 DAZKHF020000000
VERSION     DAZKHF020000712.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267800
            Sequence Read Archive: SRR30307263
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 665)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 665)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 665)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350581.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350581.2
            Genome Coverage       :: 151x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:21:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,652
            CDSs (total)                      :: 5,557
            Genes (coding)                    :: 5,320
            CDSs (with protein)               :: 5,320
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 237
            CDSs (without protein)            :: 237
            Pseudo Genes (ambiguous residues) :: 0 of 237
            Pseudo Genes (frameshifted)       :: 102 of 237
            Pseudo Genes (incomplete)         :: 130 of 237
            Pseudo Genes (internal stop)      :: 52 of 237
            Pseudo Genes (multiple problems)  :: 41 of 237
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..665
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267800-rid25106413.denovo.716"
                     /serotype="O 26:H11"
                     /isolate="2201S31241"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-01"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            122..197
                     /locus_tag="ACHOSC_RS27010"
                     /old_locus_tag="pgaptmp_005402"
     tRNA            122..197
                     /locus_tag="ACHOSC_RS27010"
                     /old_locus_tag="pgaptmp_005402"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:156..158,aa:Glu,seq:ttc)
     gene            382..>665
                     /locus_tag="ACHOSC_RS27015"
                     /old_locus_tag="pgaptmp_005403"
     rRNA            382..>665
                     /locus_tag="ACHOSC_RS27015"
                     /old_locus_tag="pgaptmp_005403"
                     /product="23S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF02541"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF02541"
BASE COUNT          192 a          149 c          172 g          152 t
ORIGIN      
        1 taaatagcaa ggcgtttacg cgttgggagt gaggctgaag agaataaggc cgttcgcttt
       61 ctattaatga aagctcaccc tacacgaaaa tatcacgcaa cgcgtgataa gcaattttcg
      121 tgtccccttc gtctagaggc ccaggacacc gccctttcac ggcggtaaca ggggttcgaa
      181 tcccctaggg gacgccactt gctggtttgt gagtgaaagt cgccgacccc aatatctcaa
      241 aacagactgt taagtcttgt ttgagatatt tgctctttaa aaatctggat caagctgaaa
      301 attgaaacac tgaacaacga aagttgttcg tgagtctctc aaattttcgc aactctgaag
      361 tgaaacatct tcgggttgtg aggttaagcg actaagcgta cacggtggat gccctggcag
      421 tcagaggcga tgaaggacgt gctaatctgc gataagcgtc ggtaaggtga tatgaaccgt
      481 tataaccggc gatttccgaa tggggaaacc cagtgtgatt cgtcacacta tcattaactg
      541 aatccatagg ttaatgaggc gaaccggggg aactgaaaca tctaagtacc ccgaggaaaa
      601 gaaatcaacc gagattcccc cagtagcggc gagcgaacgg ggagcagccc agagcctgaa
      661 tcagt
//
LOCUS       DAZKHF020000713          662 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2201S31241
            SAMN43267800-rid25106413.denovo.717, whole genome shotgun sequence.
ACCESSION   DAZKHF020000713 DAZKHF020000000
VERSION     DAZKHF020000713.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267800
            Sequence Read Archive: SRR30307263
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 662)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 662)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 662)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350581.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350581.2
            Genome Coverage       :: 151x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:21:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,652
            CDSs (total)                      :: 5,557
            Genes (coding)                    :: 5,320
            CDSs (with protein)               :: 5,320
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 237
            CDSs (without protein)            :: 237
            Pseudo Genes (ambiguous residues) :: 0 of 237
            Pseudo Genes (frameshifted)       :: 102 of 237
            Pseudo Genes (incomplete)         :: 130 of 237
            Pseudo Genes (internal stop)      :: 52 of 237
            Pseudo Genes (multiple problems)  :: 41 of 237
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..662
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267800-rid25106413.denovo.717"
                     /serotype="O 26:H11"
                     /isolate="2201S31241"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-01"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            133..321
                     /gene="dicB"
                     /locus_tag="ACHOSC_RS27020"
                     /old_locus_tag="pgaptmp_005404"
     CDS             133..321
                     /gene="dicB"
                     /locus_tag="ACHOSC_RS27020"
                     /old_locus_tag="pgaptmp_005404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division inhibition protein DicB"
                     /protein_id="HGU4551113.1"
                     /translation="METLLPNVNTSEGCFDIGVLLSNREFTEDAINMRKYEPYLLNDN
                     SILSRIALLELGIFGERQ"
     gene            318..506
                     /locus_tag="ACHOSC_RS27025"
                     /old_locus_tag="pgaptmp_005405"
     CDS             318..506
                     /locus_tag="ACHOSC_RS27025"
                     /old_locus_tag="pgaptmp_005405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1482 family protein"
                     /protein_id="HGU4551114.1"
                     /translation="MTSAFALMMTVFLITGESQNVITGIYASKESCLQARDEQKISGE
                     CLPVKKVSLYLNNETPAG"
     gene            601..>662
                     /locus_tag="ACHOSC_RS27030"
                     /old_locus_tag="pgaptmp_005406"
     CDS             601..>662
                     /locus_tag="ACHOSC_RS27030"
                     /old_locus_tag="pgaptmp_005406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001757322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease"
                     /protein_id="HGU4551115.1"
                     /translation="MSKVFICAAIPDEQAIKEEGA"
BASE COUNT          208 a          135 c          144 g          175 t
ORIGIN      
        1 cacgcggaac agttaaaacc aaaaacagtg ttatgggtgg attctctgta tccggcgtta
       61 attgttaact ggttaacgtc acctggaggc accaggcacc gcatcacaaa attcattgtt
      121 gaggacgcga taatggaaac gttattacca aacgttaata cgtctgaagg ttgttttgat
      181 attggtgttc tgctcagtaa ccgggagttt actgaagatg ccattaatat gaggaaatat
      241 gagccttatc tgctcaatga taattccata ctttcccgaa ttgctcttct tgaacttggt
      301 attttcggag aacgtcaata acttcagcat ttgcactgat gatgacggtt tttcttataa
      361 cgggtgaatc acagaatgtg attaccggaa tttatgccag taaagaatcc tgcctccagg
      421 caagagacga gcaaaaaatt tctggtgaat gcctcccggt aaaaaaagta tcgctgtacc
      481 tgaataacga aacaccggct ggataaccct ccagccatat taacaccata ccaacggatt
      541 aaaaatgcca gcaatggcag ggattcgttc accctgaaat ctgtaatgag gtaaaacaaa
      601 atgagtaaag tctttatttg cgccgccatt ccggacgaac aggcaataaa ggaagaaggt
      661 gc
//
LOCUS       DAZKHF020000714          660 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2201S31241
            SAMN43267800-rid25106413.denovo.718, whole genome shotgun sequence.
ACCESSION   DAZKHF020000714 DAZKHF020000000
VERSION     DAZKHF020000714.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267800
            Sequence Read Archive: SRR30307263
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 660)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 660)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 660)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350581.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350581.2
            Genome Coverage       :: 151x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:21:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,652
            CDSs (total)                      :: 5,557
            Genes (coding)                    :: 5,320
            CDSs (with protein)               :: 5,320
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 237
            CDSs (without protein)            :: 237
            Pseudo Genes (ambiguous residues) :: 0 of 237
            Pseudo Genes (frameshifted)       :: 102 of 237
            Pseudo Genes (incomplete)         :: 130 of 237
            Pseudo Genes (internal stop)      :: 52 of 237
            Pseudo Genes (multiple problems)  :: 41 of 237
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..660
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267800-rid25106413.denovo.718"
                     /serotype="O 26:H11"
                     /isolate="2201S31241"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-01"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
BASE COUNT          179 a          149 c          155 g          177 t
ORIGIN      
        1 cggcggtaac tggttgatat tcccgacggc ccaaaaatgg gttggctaca cccctcacaa
       61 ctacgacatt ttcgcagttt aattaatggt cagcttaatc gctaacccgc tgatattttc
      121 ggaaacctca atttgaggaa gtcggcgcgg taactcgctg aactttaagc aaagcgcaaa
      181 attgcgcttg ctgaataatc attatgatta cgcagatgat taaggaatga ccgaaggcgg
      241 aaattcgcct gtggttaatg ggggagttgc agatctgcaa ctcgaccatg aaactacgga
      301 aactacccgt agtttgggta gtaagagtaa cacccagatt ttggggctta ctcgtgatac
      361 ccaaatttgg ggtatcagtg gcaaccataa cgactttcgt tacggttgat gcttttaccc
      421 cattggggga aaggtattac gataatcata acacctaccg aacaggtaaa gcccaccagc
      481 ctgattaacg tttaaccgga aaaaaattcc ggtaggtggg gatccccata tcgacattaa
      541 cgcccctcat gaattggtgt gcttccccct ggaaagatga tccgcctgta tatttcttgt
      601 gtctatttgt tccacgttgt ttcatacagt gcaccgaacg gtgtagtcac tggtgtagtc
//
LOCUS       DAZKHF020000715          659 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2201S31241
            SAMN43267800-rid25106413.denovo.719, whole genome shotgun sequence.
ACCESSION   DAZKHF020000715 DAZKHF020000000
VERSION     DAZKHF020000715.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267800
            Sequence Read Archive: SRR30307263
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 659)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 659)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 659)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350581.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350581.2
            Genome Coverage       :: 151x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:21:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,652
            CDSs (total)                      :: 5,557
            Genes (coding)                    :: 5,320
            CDSs (with protein)               :: 5,320
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 237
            CDSs (without protein)            :: 237
            Pseudo Genes (ambiguous residues) :: 0 of 237
            Pseudo Genes (frameshifted)       :: 102 of 237
            Pseudo Genes (incomplete)         :: 130 of 237
            Pseudo Genes (internal stop)      :: 52 of 237
            Pseudo Genes (multiple problems)  :: 41 of 237
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..659
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267800-rid25106413.denovo.719"
                     /serotype="O 26:H11"
                     /isolate="2201S31241"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-01"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            complement(27..>659)
                     /locus_tag="ACHOSC_RS27035"
                     /old_locus_tag="pgaptmp_005407"
     CDS             complement(27..>659)
                     /locus_tag="ACHOSC_RS27035"
                     /old_locus_tag="pgaptmp_005407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000301789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HGU4551116.1"
                     /translation="GSVVLATKCIRELFLMHYGKVKIVDISESVVSQYLESQHKLTRT
                     RLTDIPLYLLLEPNNPALAAALITSQGFSGEATDMFLMMACLSLFETDERMSLFLSGC
                     LSSISAKVRAIIQTDISASWTLGAIALQLHMSESLLKTKLKNEGGMFSRLLLEERMRV
                     AVNMLCSRHGYGQAIAEKCGYSSRSYFISVFHRYYGFPPDRYVSRQGLDY"
BASE COUNT          200 a          163 c          127 g          169 t
ORIGIN      
        1 gccaaaaaat aataatcaga tgaaaatcaa taatcaagcc cttgcctgga tacatatctg
       61 tctggcggga agccataata gcggtgaaat acagaaataa agtaggacct gcttgaataa
      121 ccgcattttt ctgctatagc ctgtccatat ccatgccggg aacataacat atttacagca
      181 acacgcatcc gctcttccag caacaagcga ctgaacatgc ccccttcatt tttcagtttt
      241 gtctttaaca aactctcact catatgcaac tgtagagcaa tcgcaccaag cgtccagctt
      301 gctgatatat ctgtctgaat tatcgccctg actttggcac ttatgctgga taaacatcca
      361 cttaaaaaca atgacatccg ttcatctgtt tcaaacagag acaggcaggc catcataaga
      421 aacatatccg tggcctctcc ggaaaatccc tggctggtaa ttaaagccgc agccaacgca
      481 ggattgttgg gttccagcaa caggtaaagc ggaatgtcag tcagacgagt cctcgtcagc
      541 ttatgctgac tttccagata ttgacttacg acggattcgc ttatatcgac aattttaact
      601 ttgccataat gcataaggaa aagctccctg atgcatttgg tggccagaac gactgagcc
//
LOCUS       DAZKHF020000716          658 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2201S31241
            SAMN43267800-rid25106413.denovo.720, whole genome shotgun sequence.
ACCESSION   DAZKHF020000716 DAZKHF020000000
VERSION     DAZKHF020000716.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267800
            Sequence Read Archive: SRR30307263
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 658)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 658)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 658)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350581.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350581.2
            Genome Coverage       :: 151x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:21:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,652
            CDSs (total)                      :: 5,557
            Genes (coding)                    :: 5,320
            CDSs (with protein)               :: 5,320
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 237
            CDSs (without protein)            :: 237
            Pseudo Genes (ambiguous residues) :: 0 of 237
            Pseudo Genes (frameshifted)       :: 102 of 237
            Pseudo Genes (incomplete)         :: 130 of 237
            Pseudo Genes (internal stop)      :: 52 of 237
            Pseudo Genes (multiple problems)  :: 41 of 237
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..658
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267800-rid25106413.denovo.720"
                     /serotype="O 26:H11"
                     /isolate="2201S31241"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-01"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            1..321
                     /locus_tag="ACHOSC_RS27040"
                     /old_locus_tag="pgaptmp_005408"
                     /pseudo
     CDS             1..321
                     /locus_tag="ACHOSC_RS27040"
                     /old_locus_tag="pgaptmp_005408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612086.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
     misc_feature    2..66
                     /locus_tag="ACHOSC_RS27040"
                     /old_locus_tag="pgaptmp_005408"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01497"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="AL1L pseudoknot; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /pseudo
                     /db_xref="RFAM:RF01497"
BASE COUNT          173 a          130 c          172 g          183 t
ORIGIN      
        1 ctgctggata agctgcgtga gcagtacggg gtcggaccgg tatgcagtga actgcatatt
       61 gccccgtcaa cgtattacca ctgtcagcaa cagcgacatc atcctgataa acgcagtgcc
      121 cgtgcgcagc gcgatgactg gctgaagaga gagatacagc gcgtatacga tgaaaatcat
      181 caggtatacg gtgtgcgtaa agtctggcgc cagttgttac gcgaaggtat cagggtggcc
      241 agatgtacag tggcgcgcct catggcggtt atgggacttg ccggtgttct ccggggtaaa
      301 aaggtccgta ctaccgtcag ccgatttacg ggccgtaagt aacgaagttg gatgcaaatg
      361 tcagatcgtg tgcgcctgtt atggctcggc tggtaagaga gccttatagg cgcatttgaa
      421 aaaactccag ctataccgga agatatttat tattaacagg agaggtttgc ttttctgtgt
      481 aggtttagat tttgtgtttg cagggtaaac aaaactgatt aactcgtcat ttcggtaact
      541 gaaatagttt gctgtattct gtttacaatc tctttaataa ggcatttgtc acgtggctat
      601 tcatatgaaa atcatacgtt atcttacaaa tcaatcatct gagtgttata atataact
//
LOCUS       DAZKHF020000717          658 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2201S31241
            SAMN43267800-rid25106413.denovo.721, whole genome shotgun sequence.
ACCESSION   DAZKHF020000717 DAZKHF020000000
VERSION     DAZKHF020000717.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267800
            Sequence Read Archive: SRR30307263
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 658)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 658)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 658)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350581.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350581.2
            Genome Coverage       :: 151x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:21:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,652
            CDSs (total)                      :: 5,557
            Genes (coding)                    :: 5,320
            CDSs (with protein)               :: 5,320
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 237
            CDSs (without protein)            :: 237
            Pseudo Genes (ambiguous residues) :: 0 of 237
            Pseudo Genes (frameshifted)       :: 102 of 237
            Pseudo Genes (incomplete)         :: 130 of 237
            Pseudo Genes (internal stop)      :: 52 of 237
            Pseudo Genes (multiple problems)  :: 41 of 237
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..658
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267800-rid25106413.denovo.721"
                     /serotype="O 26:H11"
                     /isolate="2201S31241"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-01"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            <1..>658
                     /locus_tag="ACHOSC_RS27045"
                     /old_locus_tag="pgaptmp_005409"
     CDS             <1..>658
                     /locus_tag="ACHOSC_RS27045"
                     /old_locus_tag="pgaptmp_005409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000281525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="alpha-mannosidase"
                     /protein_id="HGU4551117.1"
                     /translation="KKQIAAGRFELQGCFWVECDLNLVSGESLIRQIMHGTRFTLQNF
                     SKKINYVWQPDVFGFPATLPQILKKSGINYIASQKLSQNKINKFNNYLFRWQGLDGSE
                     ILMHNFPEDTYDSRARARSLEYIEQNYNEKEICPYAFMVYGVGDGGAGPGEEHIERLT
                     RIRNIDGLPHVDFSRVDKFFTHADAFRESLPIISGELYFEAHQGCFTSESATKAHNRI
                     "
BASE COUNT          195 a          143 c          137 g          183 t
ORIGIN      
        1 taaaaaaaca aatcgccgca ggccgctttg agttacaagg ttgcttttgg gtcgaatgcg
       61 atttaaatct ggtaagtggt gaatcgctga taaggcaaat catgcatggg acacgattca
      121 cactgcagaa tttttcaaag aagataaatt atgtctggca accggatgtc tttggattcc
      181 ccgccacgct tccacaaatt ctcaaaaaat caggcattaa ctatattgca tcgcaaaagc
      241 tctcacaaaa caagattaat aaatttaata attacctttt ccgctggcaa ggcctggatg
      301 gctcagaaat tctgatgcat aactttcctg aagatacgta tgatagccga gcccgcgctc
      361 gctctttaga atatattgaa caaaactata atgaaaaaga aatctgtccc tatgcgttta
      421 tggtgtatgg tgtcggtgat ggcggcgctg gccccggtga ggaacatata gaacgattaa
      481 cacgcatacg caatattgat ggcttacctc atgttgattt cagccgtgtt gataagttct
      541 tcactcacgc cgacgccttt cgcgaatcct tgcccattat ttcgggtgaa ctttacttcg
      601 aggctcatca agggtgcttt acatcagagt cagcaactaa agcccataat cgaattat
//
LOCUS       DAZKHF020000718          657 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2201S31241
            SAMN43267800-rid25106413.denovo.722, whole genome shotgun sequence.
ACCESSION   DAZKHF020000718 DAZKHF020000000
VERSION     DAZKHF020000718.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267800
            Sequence Read Archive: SRR30307263
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 657)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 657)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 657)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350581.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350581.2
            Genome Coverage       :: 151x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:21:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,652
            CDSs (total)                      :: 5,557
            Genes (coding)                    :: 5,320
            CDSs (with protein)               :: 5,320
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 237
            CDSs (without protein)            :: 237
            Pseudo Genes (ambiguous residues) :: 0 of 237
            Pseudo Genes (frameshifted)       :: 102 of 237
            Pseudo Genes (incomplete)         :: 130 of 237
            Pseudo Genes (internal stop)      :: 52 of 237
            Pseudo Genes (multiple problems)  :: 41 of 237
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..657
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267800-rid25106413.denovo.722"
                     /serotype="O 26:H11"
                     /isolate="2201S31241"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-01"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            <1..>657
                     /locus_tag="ACHOSC_RS27050"
                     /old_locus_tag="pgaptmp_005410"
     CDS             <1..>657
                     /locus_tag="ACHOSC_RS27050"
                     /old_locus_tag="pgaptmp_005410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000633896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="alpha-amylase family glycosyl hydrolase"
                     /protein_id="HGU4551118.1"
                     /translation="LEDLGINGIYFCPIFTSASNHKYDTIDHFSVDPHLGGNIAFHTL
                     IEEAHKRGIKIMLDAVFNHLGADSPIWLDVVRNGANSRYADWFWIHKFPVYPDTPKSE
                     WDFKNFNYETFGNVIEMPKLNTENEECREYLLSIVRYWTQNFDIDGWRLDVANEVDHH
                     FWRDFRKVIKDIKPECYILGEIWHEGTPWLRGDQFDSLMNYPLTYGIIDYFALQDTTK
                     "
BASE COUNT          192 a          106 c          140 g          219 t
ORIGIN      
        1 tctggaagat ctgggtatta atgggattta tttttgtcct atttttacct cagcttccaa
       61 ccataaatat gacaccatcg atcattttag tgttgatcca catttaggtg ggaatattgc
      121 tttccatact ttgattgaag aagcgcataa gcgtggaata aaaatcatgt tggatgcagt
      181 attcaatcat ttaggcgctg actctccaat atggcttgac gttgtacgca atggtgccaa
      241 ttcacgttac gccgattggt tctggattca taaatttcct gtttatcccg atactccaaa
      301 aagtgaatgg gattttaaaa actttaacta tgaaaccttt ggcaatgtaa ttgaaatgcc
      361 caagctaaat actgagaatg aagagtgccg tgaatactta cttagtatag ttcgctattg
      421 gacgcaaaat tttgatattg atggttggcg tctggatgtt gcgaatgaag tggatcatca
      481 tttctggcga gatttcagaa aggtgattaa agatatcaaa cctgaatgtt atattttggg
      541 tgagatatgg catgagggca ccccctggtt gcgtggtgat cagtttgatt cattaatgaa
      601 ttatccctta acatatggga ttattgatta ttttgcgtta caggatacaa caaaaca
//
LOCUS       DAZKHF020000719          653 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2201S31241
            SAMN43267800-rid25106413.denovo.723, whole genome shotgun sequence.
ACCESSION   DAZKHF020000719 DAZKHF020000000
VERSION     DAZKHF020000719.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267800
            Sequence Read Archive: SRR30307263
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 653)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 653)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 653)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350581.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350581.2
            Genome Coverage       :: 151x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:21:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,652
            CDSs (total)                      :: 5,557
            Genes (coding)                    :: 5,320
            CDSs (with protein)               :: 5,320
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 237
            CDSs (without protein)            :: 237
            Pseudo Genes (ambiguous residues) :: 0 of 237
            Pseudo Genes (frameshifted)       :: 102 of 237
            Pseudo Genes (incomplete)         :: 130 of 237
            Pseudo Genes (internal stop)      :: 52 of 237
            Pseudo Genes (multiple problems)  :: 41 of 237
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..653
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267800-rid25106413.denovo.723"
                     /serotype="O 26:H11"
                     /isolate="2201S31241"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-01"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            1..62
                     /locus_tag="ACHOSC_RS27055"
                     /old_locus_tag="pgaptmp_005411"
                     /pseudo
     CDS             1..62
                     /locus_tag="ACHOSC_RS27055"
                     /old_locus_tag="pgaptmp_005411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_958720.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
     gene            66..257
                     /locus_tag="ACHOSC_RS27060"
                     /old_locus_tag="pgaptmp_005412"
                     /pseudo
     CDS             66..257
                     /locus_tag="ACHOSC_RS27060"
                     /old_locus_tag="pgaptmp_005412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000449473.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_process: GO:0042742 - defense response to bacterium
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="lipid II-degrading bacteriocin"
     gene            complement(265..618)
                     /gene="cmi"
                     /locus_tag="ACHOSC_RS27065"
                     /old_locus_tag="pgaptmp_005413"
     CDS             complement(265..618)
                     /gene="cmi"
                     /locus_tag="ACHOSC_RS27065"
                     /old_locus_tag="pgaptmp_005413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_104216667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin M immunity protein"
                     /protein_id="HGU4551119.1"
                     /translation="MKVISMKFIFILTIIALAAVFFWSEDKGPACYQVSDEQARTFVK
                     NDYLQRMKRWDNDVQLLGTEIPKITWEKIERSLTDVEDEKTLLVPFKAEGPEGKRMYY
                     GIYHCEEGYVEYAND"
BASE COUNT          194 a          144 c          110 g          205 t
ORIGIN      
        1 ttattatcta tcaacaacag aatgagcaat tatatccagc acaggaacat cttcttttac
       61 aatgttcata tgatgataaa tacgatttta acgccagcac tcaccgtggc atcatcggag
      121 agtcgctcac aaggctcggg gcgatgtttt ctggtaaaga gtaccagata ctgcttcctg
      181 gtgaaattca cattaaagaa agtggtaagc gagaatataa gggccgggat tccggccttt
      241 ttatgcagcc tttataacag tgtattagtc attcgcatat tcaacatatc cctcctcaca
      301 atggtatatg ccataataca ttctcttacc ctccgggcct tcagctttaa atgggacaag
      361 aagtgttttt tcatcttcaa catctgttaa acttctctca atcttttccc atgtaatttt
      421 cgggatttct gtaccaagaa gttgaacatc gttgtcccag cgtttcattc tttgcaggta
      481 atcatttttt acaaacgttc tggcctgttc atcgctgacc tgatagcagg ccggaccttt
      541 atcttcagac cagaaaaaaa cagcagcaag agcaataatc gttaaaataa aaataaattt
      601 catgctaatt actttcataa aatctccaca acttctgtcg ttcattcgat ata
//
LOCUS       DAZKHF020000720          652 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2201S31241
            SAMN43267800-rid25106413.denovo.724, whole genome shotgun sequence.
ACCESSION   DAZKHF020000720 DAZKHF020000000
VERSION     DAZKHF020000720.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267800
            Sequence Read Archive: SRR30307263
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 652)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 652)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 652)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350581.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350581.2
            Genome Coverage       :: 151x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:21:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,652
            CDSs (total)                      :: 5,557
            Genes (coding)                    :: 5,320
            CDSs (with protein)               :: 5,320
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 237
            CDSs (without protein)            :: 237
            Pseudo Genes (ambiguous residues) :: 0 of 237
            Pseudo Genes (frameshifted)       :: 102 of 237
            Pseudo Genes (incomplete)         :: 130 of 237
            Pseudo Genes (internal stop)      :: 52 of 237
            Pseudo Genes (multiple problems)  :: 41 of 237
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..652
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267800-rid25106413.denovo.724"
                     /serotype="O 26:H11"
                     /isolate="2201S31241"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-01"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            85..510
                     /locus_tag="ACHOSC_RS27070"
                     /old_locus_tag="pgaptmp_005414"
     CDS             85..510
                     /locus_tag="ACHOSC_RS27070"
                     /old_locus_tag="pgaptmp_005414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001373091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HGU4551120.1"
                     /translation="MELQDWRKEPRKNYSNEFKLRMVELASQPGASVARIAREHDIND
                     NLLFKWLRLWQNEGRISRRLQVTTSSDTGVELLPVEITPDEQKEPVAAIAPSLSTSTQ
                     TSVSAGSCKVEFRHGNMTLENPSPELLTLLIRELTGRGR"
     gene            507..581
                     /locus_tag="ACHOSC_RS27075"
                     /old_locus_tag="pgaptmp_005415"
                     /pseudo
     CDS             507..581
                     /locus_tag="ACHOSC_RS27075"
                     /old_locus_tag="pgaptmp_005415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309365.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family insertion sequence element accessory
                     protein TnpB"
BASE COUNT          185 a          160 c          161 g          146 t
ORIGIN      
        1 taagcgtaaa ctgaccgccg tatgtagcca tcagacgaaa attggtaact tagacgccca
       61 tctgatatag acggacatct aagtatggaa ttacaggact ggcgaaaaga acctcgtaaa
      121 aactattcga atgaattcaa acttcgtatg gtggaactgg catcacaacc aggagcctct
      181 gttgcccgta ttgcccggga acacgatatc aatgataacc tcctgttcaa atggctcagg
      241 ctctggcaga acgaagggcg tatatcacgg cgtcttcagg taacgacctc ttctgacact
      301 ggcgttgaat tattacctgt agaaataacg ccggatgagc agaaagaacc ggtggcggcc
      361 attgcgccgt ctttatccac ttccactcag accagtgtca gtgccggctc ctgcaaggtg
      421 gagttccgtc acggtaacat gacgctggaa aatccatcgc cagagctgct cacgctgttg
      481 atccgtgaac tgaccgggag gggacgatga tctcactccc gtcaggtacc cgcatctggc
      541 tcgttgccga cgttaccgat atgcgtaaat ccttcaacgg atacgacata tgctgaagga
      601 ggagcagtaa tggattaagt gataacaaat acccagaaat agaggttcaa at
//
LOCUS       DAZKHF020000721          648 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2201S31241
            SAMN43267800-rid25106413.denovo.725, whole genome shotgun sequence.
ACCESSION   DAZKHF020000721 DAZKHF020000000
VERSION     DAZKHF020000721.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267800
            Sequence Read Archive: SRR30307263
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 648)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 648)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 648)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350581.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350581.2
            Genome Coverage       :: 151x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:21:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,652
            CDSs (total)                      :: 5,557
            Genes (coding)                    :: 5,320
            CDSs (with protein)               :: 5,320
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 237
            CDSs (without protein)            :: 237
            Pseudo Genes (ambiguous residues) :: 0 of 237
            Pseudo Genes (frameshifted)       :: 102 of 237
            Pseudo Genes (incomplete)         :: 130 of 237
            Pseudo Genes (internal stop)      :: 52 of 237
            Pseudo Genes (multiple problems)  :: 41 of 237
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..648
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267800-rid25106413.denovo.725"
                     /serotype="O 26:H11"
                     /isolate="2201S31241"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-01"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            87..305
                     /locus_tag="ACHOSC_RS27080"
                     /old_locus_tag="pgaptmp_005416"
     CDS             87..305
                     /locus_tag="ACHOSC_RS27080"
                     /old_locus_tag="pgaptmp_005416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4014 family protein"
                     /protein_id="HGU4551121.1"
                     /translation="MTTLFRKEYPQKSRATEFLFLILFIVLMIPISPLIFVWAIGKII
                     EPVTELYTDVVWASFNTLHNKINPYKEN"
     gene            307..570
                     /locus_tag="ACHOSC_RS27085"
                     /old_locus_tag="pgaptmp_005417"
     CDS             307..570
                     /locus_tag="ACHOSC_RS27085"
                     /old_locus_tag="pgaptmp_005417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4551122.1"
                     /translation="MATLTKKERAWLNELQEVLDRCPSPKKIGFYTIGDKSIYLYDLR
                     RMDEIMEALDNRSSMDWCVAVHDMNAGFDEKILFPSSVESTAG"
     gene            581..>648
                     /locus_tag="ACHOSC_RS27090"
                     /old_locus_tag="pgaptmp_005418"
     CDS             581..>648
                     /locus_tag="ACHOSC_RS27090"
                     /old_locus_tag="pgaptmp_005418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4551123.1"
                     /translation="MTTITKERIELFIKNPVENGLTR"
BASE COUNT          203 a          119 c          147 g          179 t
ORIGIN      
        1 gaggttgatc cggttgatgc tgcaaatgaa gccatgagtt accacaacga ttaatgtcgg
       61 gaggccgccc gaaagggcgg taagaaatga ctacattatt cagaaaagaa tatccgcaaa
      121 aaagtagggc gacagaattt ttgtttctca ttctgtttat cgtattgatg ataccgatat
      181 cccctctaat ttttgtctgg gcaatcggga aaataattga gccagttact gaattgtata
      241 ccgacgttgt atgggcgtcg ttcaacacac tgcacaataa aattaatccg tataaggaaa
      301 actgatatgg caactttgac aaaaaaagaa cgggcatggt taaacgaatt acaggaagtt
      361 cttgatcgct gtccatcacc gaaaaaaatt ggcttttaca ccattggcga taaaagcatt
      421 tacctgtatg acctacgccg catggatgaa atcatggagg ctcttgataa tcgttcgtcg
      481 atggattggt gtgttgctgt tcatgatatg aatgcagggt ttgatgaaaa gattttgttc
      541 ccctcatcag ttgaaagcac tgcgggttaa ggagtaacac atgaccacta ttaccaaaga
      601 acgtattgaa ttgttcatta aaaatccggt tgaaaacggg cttacccg
//
LOCUS       DAZKHF020000722          644 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2201S31241
            SAMN43267800-rid25106413.denovo.726, whole genome shotgun sequence.
ACCESSION   DAZKHF020000722 DAZKHF020000000
VERSION     DAZKHF020000722.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267800
            Sequence Read Archive: SRR30307263
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 644)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 644)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 644)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350581.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350581.2
            Genome Coverage       :: 151x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:21:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,652
            CDSs (total)                      :: 5,557
            Genes (coding)                    :: 5,320
            CDSs (with protein)               :: 5,320
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 237
            CDSs (without protein)            :: 237
            Pseudo Genes (ambiguous residues) :: 0 of 237
            Pseudo Genes (frameshifted)       :: 102 of 237
            Pseudo Genes (incomplete)         :: 130 of 237
            Pseudo Genes (internal stop)      :: 52 of 237
            Pseudo Genes (multiple problems)  :: 41 of 237
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..644
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267800-rid25106413.denovo.726"
                     /serotype="O 26:H11"
                     /isolate="2201S31241"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-01"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            124..486
                     /locus_tag="ACHOSC_RS27095"
                     /old_locus_tag="pgaptmp_005419"
     CDS             124..486
                     /locus_tag="ACHOSC_RS27095"
                     /old_locus_tag="pgaptmp_005419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YibL family ribosome-associated protein"
                     /protein_id="HGU4551124.1"
                     /translation="MKEVEKNEIKRLSDRLDAIRHQQADLSLVEAADKYAELEKEKAT
                     LEAEIARLREVHSQKLSKEAQKLMKMPFQRAITKKEQADMGKLKKSVRGLVVVHPMTA
                     LGREMGLQEMTGFSKTAF"
BASE COUNT          184 a          151 c          158 g          151 t
ORIGIN      
        1 tgcgtagttt ttgagcatgt taatgattac gttgcagaac tattacaatg tcatcagttg
       61 tgaacaaagc acctggtcgc gcatactagg ggctataaat ttatctttat cagaagccat
      121 cacatgaaag aagtcgaaaa aaacgaaatc aaacgtctca gcgatcgcct ggacgccatc
      181 cgccaccagc aggccgatct gtcgctggtt gaagccgcag acaaatatgc cgagctggaa
      241 aaagagaaag ccacgctgga agcagaaatt gctcgcctgc gtgaggttca tagccaaaaa
      301 ctgagtaaag aagcacagaa gctgatgaag atgccgtttc agcgcgcaat taccaaaaaa
      361 gagcaggctg atatgggcaa gctgaagaaa agtgttcgcg gactggtggt tgtgcaccca
      421 atgaccgcgc tgggccgtga aatgggcctg caggagatga ctggattctc taagactgcg
      481 ttttaagcgc ctcagacgac tggttcgatc atatcaaccc gcatctggct tgccttagct
      541 ggtcaatgcg gggactctcc tgtctactcg cctaattacc aaacaattca ctccctttac
      601 gtcgatttac cgctggttga tattctgaaa atgcattttt accg
//
LOCUS       DAZKHF020000723          643 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2201S31241
            SAMN43267800-rid25106413.denovo.727, whole genome shotgun sequence.
ACCESSION   DAZKHF020000723 DAZKHF020000000
VERSION     DAZKHF020000723.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267800
            Sequence Read Archive: SRR30307263
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 643)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 643)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 643)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350581.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350581.2
            Genome Coverage       :: 151x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:21:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,652
            CDSs (total)                      :: 5,557
            Genes (coding)                    :: 5,320
            CDSs (with protein)               :: 5,320
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 237
            CDSs (without protein)            :: 237
            Pseudo Genes (ambiguous residues) :: 0 of 237
            Pseudo Genes (frameshifted)       :: 102 of 237
            Pseudo Genes (incomplete)         :: 130 of 237
            Pseudo Genes (internal stop)      :: 52 of 237
            Pseudo Genes (multiple problems)  :: 41 of 237
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..643
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267800-rid25106413.denovo.727"
                     /serotype="O 26:H11"
                     /isolate="2201S31241"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-01"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            17..580
                     /gene="fimA"
                     /locus_tag="ACHOSC_RS27100"
                     /old_locus_tag="pgaptmp_005420"
     CDS             17..580
                     /gene="fimA"
                     /locus_tag="ACHOSC_RS27100"
                     /old_locus_tag="pgaptmp_005420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbrial major subunit FimA"
                     /protein_id="HGU4551125.1"
                     /translation="MKLKHVGMIVVSVLAMSSAAVSAAEGDESVTTTVNGGVIHFKGE
                     VVNAACAIDSESMNQTVELGQVRSSRLAKAGDLSSAVGFNIKLNDCDTNVSSNAAVAF
                     LGTTVTSNDDTLALQSSAAGSAQNVGIQILDRTGEVLILDGATFSAKTDLIDGTNILP
                     FQARYIALGQSVAGTANADATFKVQYL"
BASE COUNT          159 a          127 c          166 g          191 t
ORIGIN      
        1 taaattttgg agaattatga aactcaaaca tgttggtatg attgtcgttt ctgtgttggc
       61 gatgtcgtct gctgcggtaa gcgcagccga gggtgatgaa tcagtaacga ccactgttaa
      121 tggcggtgtt attcatttta aaggtgaagt ggtaaatgcc gcttgtgcaa ttgattcaga
      181 atcaatgaac caaacggttg agctgggtca ggttcgttct tctcgcttgg ctaaagcggg
      241 tgacctcagc tccgccgttg gcttcaatat caagctgaat gattgtgata ccaatgtttc
      301 cagtaatgca gctgttgcat tcctgggtac tactgtcacc agtaatgacg atacgttagc
      361 gctgcagagt tcagcggcag gctctgccca aaatgtcggt attcaaattt tggaccgtac
      421 gggtgaggta ttaatacttg atggggccac ttttagtgct aaaaccgact tgattgatgg
      481 cacgaatata ctgccattcc aggctcgtta tattgctctc gggcagtccg tagctggtac
      541 tgcaaacgct gatgcgacct tcaaagttca atatctataa ttcaatctta aggtggctat
      601 acgtcatctg aaattgaaac agggtgtgtg tggcatggct acg
//
LOCUS       DAZKHF020000724          643 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2201S31241
            SAMN43267800-rid25106413.denovo.728, whole genome shotgun sequence.
ACCESSION   DAZKHF020000724 DAZKHF020000000
VERSION     DAZKHF020000724.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267800
            Sequence Read Archive: SRR30307263
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 643)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 643)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 643)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350581.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350581.2
            Genome Coverage       :: 151x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:21:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,652
            CDSs (total)                      :: 5,557
            Genes (coding)                    :: 5,320
            CDSs (with protein)               :: 5,320
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 237
            CDSs (without protein)            :: 237
            Pseudo Genes (ambiguous residues) :: 0 of 237
            Pseudo Genes (frameshifted)       :: 102 of 237
            Pseudo Genes (incomplete)         :: 130 of 237
            Pseudo Genes (internal stop)      :: 52 of 237
            Pseudo Genes (multiple problems)  :: 41 of 237
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..643
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267800-rid25106413.denovo.728"
                     /serotype="O 26:H11"
                     /isolate="2201S31241"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-01"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            complement(<1..104)
                     /locus_tag="ACHOSC_RS27105"
                     /old_locus_tag="pgaptmp_005421"
     CDS             complement(<1..104)
                     /locus_tag="ACHOSC_RS27105"
                     /old_locus_tag="pgaptmp_005421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease"
                     /protein_id="HGU4551126.1"
                     /translation="MNLSASMNDAGDGFELLMSGNMQDFNICCRRDNH"
BASE COUNT          202 a          156 c          127 g          158 t
ORIGIN      
        1 taatgattat cccgacgaca acaaatatta aaatcctgca tattacccga catcaggagt
       61 tcaaaaccgt ccccggcatc attcatactg gcagataaat tcattaccca gtttagtgct
      121 aatcaacagg tacggcttaa cttacaggaa ttccccggga ggggcggact catcctcagt
      181 tagcggcaaa atcgcatcac agccaatcgc ctgtctcgat tccccatcgg cgaacaatgt
      241 atgtggccac aaaatcctgg ctggctcaga acgaagagca tctgagcaaa gaggtcatca
      301 atctggtatg aatatctggc agaaaatatc ccctgaatcc acaactgctg tgcatatctg
      361 ctcaaatcag catcaatcat aaaccagtaa tcacagtgcc tgtgattggg ttacaggaat
      421 aagtccccct tcataaggca tatcatcaat accggtgaag gaaaagaaag catcagatgg
      481 cctgatagtc tgcgtcagtc aatgacatca gtgctttatc cggaacaatg tatccatgtt
      541 ggcgacagag aaagccattg ccatgaacag ttttgttcat ccccggaaag tcatttctgt
      601 tacaaattta tattgacaaa cttgctttcg atgagaaaca tac
//
LOCUS       DAZKHF020000725          642 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2201S31241
            SAMN43267800-rid25106413.denovo.729, whole genome shotgun sequence.
ACCESSION   DAZKHF020000725 DAZKHF020000000
VERSION     DAZKHF020000725.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267800
            Sequence Read Archive: SRR30307263
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 642)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 642)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 642)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350581.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350581.2
            Genome Coverage       :: 151x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:21:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,652
            CDSs (total)                      :: 5,557
            Genes (coding)                    :: 5,320
            CDSs (with protein)               :: 5,320
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 237
            CDSs (without protein)            :: 237
            Pseudo Genes (ambiguous residues) :: 0 of 237
            Pseudo Genes (frameshifted)       :: 102 of 237
            Pseudo Genes (incomplete)         :: 130 of 237
            Pseudo Genes (internal stop)      :: 52 of 237
            Pseudo Genes (multiple problems)  :: 41 of 237
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..642
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267800-rid25106413.denovo.729"
                     /serotype="O 26:H11"
                     /isolate="2201S31241"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-01"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            complement(<1..62)
                     /locus_tag="ACHOSC_RS27110"
                     /old_locus_tag="pgaptmp_005422"
     CDS             complement(<1..62)
                     /locus_tag="ACHOSC_RS27110"
                     /old_locus_tag="pgaptmp_005422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001059701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="condensation protein"
                     /protein_id="HGU4551127.1"
                     /translation="MNNILLPPINIPCTLFETIS"
     gene            243..395
                     /locus_tag="ACHOSC_RS27115"
                     /old_locus_tag="pgaptmp_005423"
                     /pseudo
     CDS             243..395
                     /locus_tag="ACHOSC_RS27115"
                     /old_locus_tag="pgaptmp_005423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000947142.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="CorA family divalent cation transporter"
     gene            complement(420..>642)
                     /locus_tag="ACHOSC_RS27120"
                     /old_locus_tag="pgaptmp_005424"
     CDS             complement(420..>642)
                     /locus_tag="ACHOSC_RS27120"
                     /old_locus_tag="pgaptmp_005424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001349388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4551128.1"
                     /translation="NINIGAKRIRDTGCKAIYFVLISLGLTLFSIISAAIEGVRCESL
                     SVQLNVAWILFTTIMLSAIPSAKMSLSAH"
BASE COUNT          197 a          117 c          124 g          204 t
ORIGIN      
        1 aaactaatag tttcgaaaag tgtacaggga atattaattg gcggaagtaa tatgttattc
       61 atttgcattt gccttggtta tatttaagct caatatcaat tcgagaacaa atgctagggt
      121 aaaaaaatat ttaaaaaaga agtaaacttc acattttttc attacatctc tgaagagaac
      181 ctcgttaatg attggtcttg ctcattgtta ataacaaaat gttgaattta ccgattagga
      241 aactttttcc tacgcggatt gcctccagct atggcatgaa ctttgagttt atgccagaac
      301 tgaagtgggg cttcggctac cctggcgcga ttatctttat gatcctcgcg ggcctgacac
      361 cgtatctgta ctttaagcgt aagaactggc tgtaactata cgatgtcgct gtgagtaagt
      421 tagtgtgctg acagcgacat tttagccgag ggaatcgcag ataacataat tgttgtgaat
      481 aatatccatg ccacgttgag ttggacgctt aatgactcac atctcacccc ttcaattgct
      541 gcagagataa tagaaaaaag tgtcaagccc aatgaaatca atacaaagta gattgcctta
      601 catccagtat ctcttattct ttttgctcca atattgatat tt
//
LOCUS       DAZKHF020000726          642 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2201S31241
            SAMN43267800-rid25106413.denovo.730, whole genome shotgun sequence.
ACCESSION   DAZKHF020000726 DAZKHF020000000
VERSION     DAZKHF020000726.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267800
            Sequence Read Archive: SRR30307263
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 642)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 642)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 642)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350581.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350581.2
            Genome Coverage       :: 151x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:21:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,652
            CDSs (total)                      :: 5,557
            Genes (coding)                    :: 5,320
            CDSs (with protein)               :: 5,320
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 237
            CDSs (without protein)            :: 237
            Pseudo Genes (ambiguous residues) :: 0 of 237
            Pseudo Genes (frameshifted)       :: 102 of 237
            Pseudo Genes (incomplete)         :: 130 of 237
            Pseudo Genes (internal stop)      :: 52 of 237
            Pseudo Genes (multiple problems)  :: 41 of 237
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..642
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267800-rid25106413.denovo.730"
                     /serotype="O 26:H11"
                     /isolate="2201S31241"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-01"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            244..459
                     /gene="essD"
                     /locus_tag="ACHOSC_RS27125"
                     /old_locus_tag="pgaptmp_005425"
     CDS             244..459
                     /gene="essD"
                     /locus_tag="ACHOSC_RS27125"
                     /old_locus_tag="pgaptmp_005425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage lysis protein EssD"
                     /protein_id="HGU4551129.1"
                     /translation="MKSMDKLTTGVAYGTSAGSAGYWFLQLLDKVTPSQWAAIGVLGS
                     LVFGLLTYLTNLYFKIKEDKRKAARGE"
     gene            459..>642
                     /gene="rrrD"
                     /locus_tag="ACHOSC_RS27130"
                     /old_locus_tag="pgaptmp_005426"
     CDS             459..>642
                     /gene="rrrD"
                     /locus_tag="ACHOSC_RS27130"
                     /old_locus_tag="pgaptmp_005426"
                     /EC_number="3.2.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysozyme RrrD"
                     /protein_id="HGU4551130.1"
                     /translation="MPPSLRKAVAAAIGGGAIAIASVLITGPSGNDGLEGVSYIPYKD
                     IVGVWTVCHGHTGKDIM"
BASE COUNT          161 a          134 c          157 g          190 t
ORIGIN      
        1 attctcgcaa atatcattta gtggtttacg tacgtaaaaa attggttatg ctgttaagag
       61 tggttacttc gtcacacagc ttaaacccgc cgtcgagcgg gtttttccat tttttgagtc
      121 tcgatattag ctgataaccc aatacctgag ttattcactg actccgagtc tgttacgttt
      181 ctgctttttt gcgatacgtt gtattccctc aatttacacc cgctttgtct gcgaggtggg
      241 gttatgaaat ccatggataa gttaacaacg ggtgtcgcct atggcacctc agcaggtagt
      301 gccggttact ggtttttaca gctgctcgat aaagtcacgc cctcacagtg ggcagcaata
      361 ggtgtgctgg gtagcctggt atttggcctg ctgacgtacc tgacaaacct ttatttcaag
      421 attaaagaag ataagcgcaa ggctgcgaga ggtgaataat gcctccatca ttacgaaaag
      481 ccgttgctgc tgctattggt ggcggagcaa ttgctatagc atcagtgtta atcactggcc
      541 caagtggtaa cgatggtctg gaaggtgtca gctacatacc atacaaagat attgttggtg
      601 tatggactgt atgtcacggg catacaggaa aagacatcat gc
//
LOCUS       DAZKHF020000727          640 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2201S31241
            SAMN43267800-rid25106413.denovo.731, whole genome shotgun sequence.
ACCESSION   DAZKHF020000727 DAZKHF020000000
VERSION     DAZKHF020000727.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267800
            Sequence Read Archive: SRR30307263
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 640)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 640)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 640)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350581.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350581.2
            Genome Coverage       :: 151x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:21:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,652
            CDSs (total)                      :: 5,557
            Genes (coding)                    :: 5,320
            CDSs (with protein)               :: 5,320
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 237
            CDSs (without protein)            :: 237
            Pseudo Genes (ambiguous residues) :: 0 of 237
            Pseudo Genes (frameshifted)       :: 102 of 237
            Pseudo Genes (incomplete)         :: 130 of 237
            Pseudo Genes (internal stop)      :: 52 of 237
            Pseudo Genes (multiple problems)  :: 41 of 237
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..640
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267800-rid25106413.denovo.731"
                     /serotype="O 26:H11"
                     /isolate="2201S31241"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-01"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            <1..>640
                     /locus_tag="ACHOSC_RS27135"
                     /old_locus_tag="pgaptmp_005427"
     CDS             <1..>640
                     /locus_tag="ACHOSC_RS27135"
                     /old_locus_tag="pgaptmp_005427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001239084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4551131.1"
                     /translation="VLFPPVTSGLSGQEKQKKPKSITGFQENYQRNIRPIKTASEARL
                     RFFDKMVSKENSLEDVVSLGEMIQKEIYGHEQRTFSPVHHTGNWKSSLLHNALLGLAN
                     VYNGLRETEYPNTFNRDGIKSTNSFRDNLLTKTRTPRDNFEEGIKHPEHATIPYDNDN
                     ESNKLLKAGKIAGNNNELLMEIKKESKSDHQIPLLKRKKRSPVAEDKVQNSLT"
BASE COUNT          250 a          121 c          118 g          151 t
ORIGIN      
        1 agttcttttt cctcctgtca ctagtggcct gtcagggcag gaaaaacaaa aaaaaccgaa
       61 gagcattacc ggatttcagg aaaattatca acgcaatatc aggccaatca aaacagcatc
      121 agaagcccga ctacgcttct ttgataaaat ggtttcgaaa gaaaactctc tggaagatgt
      181 tgtttcttta ggtgaaatga ttcagaagga aatttatggg catgaacaaa gaacattttc
      241 accagttcat catacaggta actggaaatc atcattgtta cacaacgcgc tccttggtct
      301 ggcaaatgtt tataatggct tacgggaaac agaataccct aacactttca acagagatgg
      361 tataaaaagt actaactctt ttagagataa cttattgaca aaaacaagaa ctcccagaga
      421 taattttgag gaaggaataa aacatcctga acatgcaaca ataccatatg acaacgacaa
      481 tgaaagtaat aaattgctaa aagcaggaaa gatagctggt aacaataacg agctgttgat
      541 ggaaataaaa aaggaatcca aaagcgacca tcaaatcccc ctcctgaaaa ggaaaaaacg
      601 gtctcctgta gctgaagata aagttcaaaa ctcgttaaca
//
LOCUS       DAZKHF020000728          638 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2201S31241
            SAMN43267800-rid25106413.denovo.732, whole genome shotgun sequence.
ACCESSION   DAZKHF020000728 DAZKHF020000000
VERSION     DAZKHF020000728.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267800
            Sequence Read Archive: SRR30307263
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 638)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 638)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 638)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350581.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350581.2
            Genome Coverage       :: 151x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:21:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,652
            CDSs (total)                      :: 5,557
            Genes (coding)                    :: 5,320
            CDSs (with protein)               :: 5,320
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 237
            CDSs (without protein)            :: 237
            Pseudo Genes (ambiguous residues) :: 0 of 237
            Pseudo Genes (frameshifted)       :: 102 of 237
            Pseudo Genes (incomplete)         :: 130 of 237
            Pseudo Genes (internal stop)      :: 52 of 237
            Pseudo Genes (multiple problems)  :: 41 of 237
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..638
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267800-rid25106413.denovo.732"
                     /serotype="O 26:H11"
                     /isolate="2201S31241"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-01"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            complement(<1..>638)
                     /gene="iha"
                     /locus_tag="ACHOSC_RS27140"
                     /old_locus_tag="pgaptmp_005428"
     CDS             complement(<1..>638)
                     /gene="iha"
                     /locus_tag="ACHOSC_RS27140"
                     /old_locus_tag="pgaptmp_005428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional siderophore receptor/adhesin Iha"
                     /protein_id="HGU4551132.1"
                     /translation="GMPASYTLILIDGVRQGGSSDVTPNGFSAMNTGFMPPLAAIERI
                     EVIRGPMSTLYGSDAMGGVVNIITRKNADKWLSSVNAGLNLQESNKWGNSSQFNFWSS
                     GPLVDDSVSLQVRGSTQQRQGSSVTSLSDTAATRIPYPTESQNYNLGARLDWKASEQD
                     VLWFDMDTTRQRYDNRDGQLGSLTGGYDRTLRYERNKISAGYDHTFTFGTWKS"
BASE COUNT          151 a          183 c          150 g          154 t
ORIGIN      
        1 gatttccatg ttccgaaggt gaaagtatga tcatagccag ctgaaatttt gtttcgctca
       61 tagcgcaggg tccggtcata tccccccgtc agactcccca gttgcccatc ccggttatca
      121 taacgctgcc gggtggtatc catatcaaac cagagcacat cctgctccga cgccttccag
      181 tcaagacgtg caccaagatt ataattctgt gactccgtgg gataaggaat acgcgtggct
      241 gctgtatcgc tcagtgatgt gaccgatgaa ccctgacgct gttgtgtgct accgcgtacc
      301 tgcaggctga cagaatcatc cacaagggga ccactgctcc agaaattaaa ctggctgctg
      361 ttaccccatt tgttgctttc ctgcagattc agccctgcat tgacggaaga gagccatttg
      421 tctgcattct ttctggtaat gatattcacc acaccgccca tcgcatcaga gccatacagt
      481 gtggacatcg gccccctgat aacctcaata cgctcaatgg cggccagagg gggcatgaac
      541 ccggtattca tggcagaaaa accgttggga gtcacgtcac tgcttccgcc ctgacgaaca
      601 ccatcaatca gtatcagcgt gtaactggct ggcattcc
//
LOCUS       DAZKHF020000729          637 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2201S31241
            SAMN43267800-rid25106413.denovo.733, whole genome shotgun sequence.
ACCESSION   DAZKHF020000729 DAZKHF020000000
VERSION     DAZKHF020000729.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267800
            Sequence Read Archive: SRR30307263
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 637)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 637)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 637)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350581.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350581.2
            Genome Coverage       :: 151x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:21:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,652
            CDSs (total)                      :: 5,557
            Genes (coding)                    :: 5,320
            CDSs (with protein)               :: 5,320
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 237
            CDSs (without protein)            :: 237
            Pseudo Genes (ambiguous residues) :: 0 of 237
            Pseudo Genes (frameshifted)       :: 102 of 237
            Pseudo Genes (incomplete)         :: 130 of 237
            Pseudo Genes (internal stop)      :: 52 of 237
            Pseudo Genes (multiple problems)  :: 41 of 237
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..637
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267800-rid25106413.denovo.733"
                     /serotype="O 26:H11"
                     /isolate="2201S31241"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-01"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            <1..>637
                     /locus_tag="ACHOSC_RS27145"
                     /old_locus_tag="pgaptmp_005429"
     CDS             <1..>637
                     /locus_tag="ACHOSC_RS27145"
                     /old_locus_tag="pgaptmp_005429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS3-like element IS1203 family transposase"
                     /protein_id="HGU4551133.1"
                     /translation="DWLKREIQRVYDENHQVYGVRKVWRQLLREGIRVARCTVARLMA
                     VMGLAGVLRGKKVRTTISRKAVAAGDRVNRQFVAERPDQLWVADFTYVSTWQGFVYVA
                     FIIDVFAGYIVGWRVSSSMETTFVLDALEQALWARRPSGTIHHSDKGSQYVSLAYTER
                     LKEAGLLASTGSTGDSYDNAMAESINGLYKAEVIHRKSWKNRAEVELATLTW"
BASE COUNT          153 a          143 c          202 g          139 t
ORIGIN      
        1 gactggctga agagagagat acagcgcgta tacgatgaaa atcatcaggt gtacggtgtg
       61 cgtaaagtct ggcgtcagtt gttacgggaa ggaatcaggg tggccagatg tacagtggca
      121 cgtctcatgg cggttatggg acttgccggt gttctccggg gtaaaaaggt ccgtacgacc
      181 atcagccgga aagccgttgc cgcaggcgac cgcgtaaacc gtcagttcgt ggcagaacga
      241 cctgaccagc tgtgggtggc tgattttact tacgtcagca catggcaggg cttcgtctat
      301 gtggcgttta tcattgatgt gtttgccgga tacatcgtgg ggtggcgggt ctcatcgtct
      361 atggaaacga cattcgtgct ggatgcgctg gagcaggcgt tgtgggcccg tcgtccgtct
      421 ggcaccatcc atcacagcga taaaggctct cagtatgtgt cactggccta tacggagcga
      481 ctaaaagaag ccggattact ggcatcaaca gggagtacag gcgactcgta tgacaacgcg
      541 atggctgaga gcatcaatgg tctttacaaa gcggaggtaa tacaccgtaa gagctggaaa
      601 aaccgtgcag aagtggaact ggccacacta acgtggg
//
LOCUS       DAZKHF020000730          635 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2201S31241
            SAMN43267800-rid25106413.denovo.734, whole genome shotgun sequence.
ACCESSION   DAZKHF020000730 DAZKHF020000000
VERSION     DAZKHF020000730.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267800
            Sequence Read Archive: SRR30307263
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 635)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 635)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 635)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350581.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350581.2
            Genome Coverage       :: 151x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:21:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,652
            CDSs (total)                      :: 5,557
            Genes (coding)                    :: 5,320
            CDSs (with protein)               :: 5,320
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 237
            CDSs (without protein)            :: 237
            Pseudo Genes (ambiguous residues) :: 0 of 237
            Pseudo Genes (frameshifted)       :: 102 of 237
            Pseudo Genes (incomplete)         :: 130 of 237
            Pseudo Genes (internal stop)      :: 52 of 237
            Pseudo Genes (multiple problems)  :: 41 of 237
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..635
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267800-rid25106413.denovo.734"
                     /serotype="O 26:H11"
                     /isolate="2201S31241"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-01"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            complement(<1..>635)
                     /locus_tag="ACHOSC_RS27150"
                     /old_locus_tag="pgaptmp_005430"
     CDS             complement(<1..>635)
                     /locus_tag="ACHOSC_RS27150"
                     /old_locus_tag="pgaptmp_005430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000242972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="porin"
                     /protein_id="HGU4551134.1"
                     /translation="STGAFAANDRGFTSDTDWSEEQAGFNGHVGTTFEYETKSTDNYL
                     GKTVKEYTKTHEIFQAYFSQPNWQFAAIYGLKSIDRRQEEKGYHENETSLQNYISLNK
                     TFTIGNGFDFSLVYDLMYTDGNIDSPYVTGLHTVTVDNSFRPTLTYFNSDWNAGFYSN
                     VEYLYSRNDKSSWGVREEEGQSILFQPYKRFGAWEFGIEFFYQKKHNDEFN"
BASE COUNT          171 a          129 c          127 g          208 t
ORIGIN      
        1 gattaaattc atcgttatgc tttttctggt agaaaaattc aataccaaac tcccatgcac
       61 caaaacgttt atagggctga aatagtatac tttgaccttc ttcttcgcgt acgccccagg
      121 agctcttatc atttcgactg tataagtatt caacgtttga ataaaaacct gcgttccagt
      181 cagagttaaa gtacgtcaac gtagggcgaa aactgttgtc aacagttaca gtatgcaagc
      241 ctgtaacata agggctatct atatttccgt cggtatacat caggtcgtat accagactga
      301 agtcgaaacc gttaccaata gtgaacgttt tgtttagtga tatatagttt tgcagacttg
      361 tttcattttc gtgatagcct ttttcttctt gccggcgatc gatagactta aggccataga
      421 ttgctgcaaa ttgccaatta ggttggctga aataggcctg gaaaatctcg tgtgttttag
      481 tatattcctt tactgtttta cctaaatagt tatcagtgct tttggtctca tattcaaatg
      541 tagtaccaac atgtccatta aagcccgctt gttcttctga ccagtcagtg tcggaggtaa
      601 agccgcgatc gtttgccgca aaggctcctg ttgag
//
LOCUS       DAZKHF020000731          630 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2201S31241
            SAMN43267800-rid25106413.denovo.735, whole genome shotgun sequence.
ACCESSION   DAZKHF020000731 DAZKHF020000000
VERSION     DAZKHF020000731.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267800
            Sequence Read Archive: SRR30307263
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 630)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 630)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 630)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350581.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350581.2
            Genome Coverage       :: 151x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:21:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,652
            CDSs (total)                      :: 5,557
            Genes (coding)                    :: 5,320
            CDSs (with protein)               :: 5,320
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 237
            CDSs (without protein)            :: 237
            Pseudo Genes (ambiguous residues) :: 0 of 237
            Pseudo Genes (frameshifted)       :: 102 of 237
            Pseudo Genes (incomplete)         :: 130 of 237
            Pseudo Genes (internal stop)      :: 52 of 237
            Pseudo Genes (multiple problems)  :: 41 of 237
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..630
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267800-rid25106413.denovo.735"
                     /serotype="O 26:H11"
                     /isolate="2201S31241"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-01"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            <1..>630
                     /locus_tag="ACHOSC_RS27155"
                     /old_locus_tag="pgaptmp_005431"
     CDS             <1..>630
                     /locus_tag="ACHOSC_RS27155"
                     /old_locus_tag="pgaptmp_005431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001239084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TcdA/TcdB catalytic glycosyltransferase
                     domain-containing protein"
                     /protein_id="HGU4551135.1"
                     /translation="DSIFTKINTRIPRDTMPILRRYHLWPDGWNIRGLNGFMLSHKGS
                     EVIDAVIAGQNQAYRELRRIRDNIHSEIYFKQTDELSSLPDTDKIGGILVKKYLSGSL
                     FSKFRQDTIIPEALSTLQISGPDLIQRKMLQFFRSRGVLGEEFINERKLSDKAYIGVY
                     KTTGTGKYDWLNPESIGVNDVTPADESTWCIGKGRCVDDFLFKDVSKLKT"
BASE COUNT          212 a          109 c          139 g          170 t
ORIGIN      
        1 gatagcattt ttactaagat caatacaagg attcctcgag acacaatgcc catccttcgt
       61 cgttatcacc tatggcctga tggatggaat attcgtgggc tcaatggatt catgctatca
      121 cataaaggta gtgaagtgat tgatgctgtt atcgcaggcc agaatcaggc ttacagggaa
      181 ctaagaagaa taagagataa tattcatagt gaaatatact tcaaacaaac tgatgaattg
      241 tcctcacttc cagatacaga caaaattgga gggattctgg taaaaaaata cctttcagga
      301 agtctttttt caaaattcag acaagatact attatcccgg aggcattgag caccctccag
      361 atatcaggtc ctgacttgat tcaaagaaag atgttgcaat ttttcaggag tagaggggtg
      421 ttaggtgaag agttcattaa tgaaagaaaa ctgagtgata aagcttatat tggtgtctac
      481 aaaacaactg gcacagggaa atatgactgg ttaaaccctg aatcgatcgg cgttaatgat
      541 gtcacgcccg cagatgaaag tacctggtgt ataggaaaag gccggtgtgt tgatgacttc
      601 ctgttcaaag atgtttcaaa actaaaaaca
//
LOCUS       DAZKHF020000732          624 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2201S31241
            SAMN43267800-rid25106413.denovo.736, whole genome shotgun sequence.
ACCESSION   DAZKHF020000732 DAZKHF020000000
VERSION     DAZKHF020000732.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267800
            Sequence Read Archive: SRR30307263
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 624)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 624)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 624)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350581.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350581.2
            Genome Coverage       :: 151x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:21:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,652
            CDSs (total)                      :: 5,557
            Genes (coding)                    :: 5,320
            CDSs (with protein)               :: 5,320
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 237
            CDSs (without protein)            :: 237
            Pseudo Genes (ambiguous residues) :: 0 of 237
            Pseudo Genes (frameshifted)       :: 102 of 237
            Pseudo Genes (incomplete)         :: 130 of 237
            Pseudo Genes (internal stop)      :: 52 of 237
            Pseudo Genes (multiple problems)  :: 41 of 237
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..624
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267800-rid25106413.denovo.736"
                     /serotype="O 26:H11"
                     /isolate="2201S31241"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-01"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            complement(149..418)
                     /locus_tag="ACHOSC_RS27160"
                     /old_locus_tag="pgaptmp_005432"
     CDS             complement(149..418)
                     /locus_tag="ACHOSC_RS27160"
                     /old_locus_tag="pgaptmp_005432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310967.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail fiber C-terminal domain-containing
                     protein"
                     /protein_id="HGU4551136.1"
                     /translation="MNILKKLMQRLCGCGKHDGREHVQSLTAQLRLGPADILESDENG
                     IIPEQARVITQVVILDADKKQIQCVVRPLQILRADGTWENIGGMK"
     gene            complement(420..>624)
                     /locus_tag="ACHOSC_RS27165"
                     /old_locus_tag="pgaptmp_005433"
     CDS             complement(420..>624)
                     /locus_tag="ACHOSC_RS27165"
                     /old_locus_tag="pgaptmp_005433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="phage tail fiber C-terminal domain-containing
                     protein"
                     /protein_id="HGU4551137.1"
                     /translation="NIIETNSHGWFPDTDGALITGLTFLDPKDATRVQGFFQHLQVRF
                     GDGPWQDVKGLDEVGSDTGRTGE"
BASE COUNT          146 a          179 c          123 g          176 t
ORIGIN      
        1 attaatacat ctaagcgctc gtgattataa tataattacc tgtattagct ctgacctgac
       61 agttaccggt tatttataca ggtatctgtc agattacatc taaccaacaa aaaccggagc
      121 cggactccgg tttttgtgaa gctgtcggct atttcattcc gccaatattt tcccacgtcc
      181 cgtcagcacg caggatttgc agcggtctta ccacacactg tatctgcttt ttatccgcat
      241 ccagtatcac cacctgcgtg attaccctgg cctgctccgg gataatgcca ttctcatcgg
      301 actccagaat gtctgccggt cccagtcgca gctgtgctgt aagcgactgc acgtgttcac
      361 ggccatcatg ctttccgcaa ccacacagac gctgcataag tttttttagt atattcatgt
      421 cattctcctg ttctgcctgt atcactgccc acttcatcaa gccccttaac atcctgccac
      481 ggcccgtcac caaacctgac ctgcaaatgc tgaaaaaaac cctgaacccg tgtggcatct
      541 ttggggtcaa gaaaggtcag tccggtgatg agcgcaccat ctgtatccgg gaaccagcca
      601 tggctgtttg tctcaataat gttt
//
LOCUS       DAZKHF020000733          622 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2201S31241
            SAMN43267800-rid25106413.denovo.737, whole genome shotgun sequence.
ACCESSION   DAZKHF020000733 DAZKHF020000000
VERSION     DAZKHF020000733.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267800
            Sequence Read Archive: SRR30307263
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 622)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 622)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 622)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350581.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350581.2
            Genome Coverage       :: 151x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:21:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,652
            CDSs (total)                      :: 5,557
            Genes (coding)                    :: 5,320
            CDSs (with protein)               :: 5,320
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 237
            CDSs (without protein)            :: 237
            Pseudo Genes (ambiguous residues) :: 0 of 237
            Pseudo Genes (frameshifted)       :: 102 of 237
            Pseudo Genes (incomplete)         :: 130 of 237
            Pseudo Genes (internal stop)      :: 52 of 237
            Pseudo Genes (multiple problems)  :: 41 of 237
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..622
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267800-rid25106413.denovo.737"
                     /serotype="O 26:H11"
                     /isolate="2201S31241"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-01"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            19..564
                     /locus_tag="ACHOSC_RS27170"
                     /old_locus_tag="pgaptmp_005434"
     CDS             19..564
                     /locus_tag="ACHOSC_RS27170"
                     /old_locus_tag="pgaptmp_005434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-packaging protein"
                     /protein_id="HGU4551138.1"
                     /translation="MEVNKKQLADIFGASIRTIQNWQEQGMPVLRGGGKGNEVLYDSA
                     AVIKWYAERDAEIENEKLRREVEELRQASETDLQPGTIEYERHRLTRAQADAQELKNA
                     RDSAEVVETAFCTFVLSRIAGEIASILDGIPLSVQRRFPELENRHVDFLKRDIIKAMN
                     KAAALDELIPGLLSEYIEQSG"
BASE COUNT          160 a          148 c          187 g          127 t
ORIGIN      
        1 ttgtccgtgg aatgaacaat ggaagtcaac aaaaagcagc tggctgacat tttcggtgcg
       61 agtatccgta ccattcagaa ctggcaggaa cagggaatgc ccgttctgcg aggcggtggc
      121 aagggtaatg aggtgcttta tgactctgcc gccgtcataa aatggtatgc cgaaagggat
      181 gctgaaattg agaacgaaaa gctgcgccgg gaagttgaag aactgcggca ggccagcgag
      241 acagatctcc agccagggac tattgagtac gaacgccatc gacttacgcg tgcgcaggcc
      301 gacgcacagg agctgaaaaa tgccagagac tccgctgaag tggtggaaac cgcattctgt
      361 actttcgtgc tgtcgcggat cgcaggtgaa attgccagta ttctcgacgg gatccccctg
      421 tcggtgcagc ggcgttttcc ggaactggaa aaccgacatg ttgatttcct gaaacgggat
      481 atcatcaaag ccatgaacaa agcagccgcg ctggatgaac tgataccggg gttgctgagt
      541 gaatatatcg aacagtcagg ttaacaggct gcggcatttt gtccgcgccg ggcttcgctc
      601 actgttcagg ccggagccac ag
//
LOCUS       DAZKHF020000734          620 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2201S31241
            SAMN43267800-rid25106413.denovo.738, whole genome shotgun sequence.
ACCESSION   DAZKHF020000734 DAZKHF020000000
VERSION     DAZKHF020000734.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267800
            Sequence Read Archive: SRR30307263
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 620)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 620)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 620)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350581.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350581.2
            Genome Coverage       :: 151x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:21:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,652
            CDSs (total)                      :: 5,557
            Genes (coding)                    :: 5,320
            CDSs (with protein)               :: 5,320
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 237
            CDSs (without protein)            :: 237
            Pseudo Genes (ambiguous residues) :: 0 of 237
            Pseudo Genes (frameshifted)       :: 102 of 237
            Pseudo Genes (incomplete)         :: 130 of 237
            Pseudo Genes (internal stop)      :: 52 of 237
            Pseudo Genes (multiple problems)  :: 41 of 237
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..620
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267800-rid25106413.denovo.738"
                     /serotype="O 26:H11"
                     /isolate="2201S31241"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-01"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            <1..539
                     /locus_tag="ACHOSC_RS27175"
                     /old_locus_tag="pgaptmp_005435"
     CDS             <1..539
                     /locus_tag="ACHOSC_RS27175"
                     /old_locus_tag="pgaptmp_005435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="fimbrial-like protein"
                     /protein_id="HGU4551139.1"
                     /translation="KSLLVAGVAMILSNNVFADEGHGIVKFKGEVISAPCSIKPGDED
                     LTVNLGEVADTVLKSDQKSLAEPFTIHLQDCMLSQGGTTYSKAKVTFTTANTMTGQTD
                     LLKNTKETEIGGATGVGVRILDSQSGEVTLGTPVVITFNNTNSYQELNFKARMESPSK
                     DATPGNVYAQADYKIAYE"
BASE COUNT          195 a          131 c          138 g          156 t
ORIGIN      
        1 aaaagtcact tcttgttgct ggcgtagcaa tgattctttc caataacgta tttgctgacg
       61 aaggtcacgg cattgttaaa tttaaaggtg aagttatctc tgctccatgt tcaattaaac
      121 caggtgacga agacttaact gtaaatctgg gtgaagtagc agataccgtt ctgaaaagcg
      181 accagaaatc tttggctgag cctttcacta ttcacctgca agactgcatg ttgagccagg
      241 gtggaaccac ttattcaaaa gcgaaggtta ctttcaccac agcgaatact atgacgggtc
      301 agactgacct gctgaaaaac accaaagaaa ctgaaatcgg tggtgccaca ggcgttggcg
      361 tacgtattct ggatagccag agcggcgaag tgactttagg tactccggta gtaattacat
      421 tcaacaatac taacagctat caggaactga acttcaaagc tcgtatggaa tccccaagca
      481 aagatgctac cccgggcaac gtttacgctc aggctgacta taagattgct tacgagtaat
      541 cacattaggc cgggttcgcc cggccacatc attaacggat taatgataag tggaacagat
      601 gtataaaaaa ttaaaattaa
//
LOCUS       DAZKHF020000735          616 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2201S31241
            SAMN43267800-rid25106413.denovo.739, whole genome shotgun sequence.
ACCESSION   DAZKHF020000735 DAZKHF020000000
VERSION     DAZKHF020000735.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267800
            Sequence Read Archive: SRR30307263
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 616)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 616)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 616)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350581.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350581.2
            Genome Coverage       :: 151x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:21:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,652
            CDSs (total)                      :: 5,557
            Genes (coding)                    :: 5,320
            CDSs (with protein)               :: 5,320
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 237
            CDSs (without protein)            :: 237
            Pseudo Genes (ambiguous residues) :: 0 of 237
            Pseudo Genes (frameshifted)       :: 102 of 237
            Pseudo Genes (incomplete)         :: 130 of 237
            Pseudo Genes (internal stop)      :: 52 of 237
            Pseudo Genes (multiple problems)  :: 41 of 237
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..616
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267800-rid25106413.denovo.739"
                     /serotype="O 26:H11"
                     /isolate="2201S31241"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-01"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            complement(<1..>616)
                     /locus_tag="ACHOSC_RS27180"
                     /old_locus_tag="pgaptmp_005436"
     CDS             complement(<1..>616)
                     /locus_tag="ACHOSC_RS27180"
                     /old_locus_tag="pgaptmp_005436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000582547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HGU4551140.1"
                     /translation="FVKIRMPEDVFLASLDKYNIDFALCSCGSAVEVDHDQNPIPDED
                     QVTQHDNNERMLRLVRQHSKRIGAFMWIKPRLESCDQDFEDMIASNRDIIYGIKVHPY
                     HSKMAFNSDKVQEYIRLAQKYDLPVVSHTSNDYESSPQLVYEMALKYPNVNFVMCHMG
                     LATDNQEAIELIAKLPNLYGDTAWVRADMVYQSIKICGSDKILFG"
BASE COUNT          193 a          133 c          105 g          185 t
ORIGIN      
        1 ccaaataaaa tcttatcact accacagatt tttatcgact ggtaaaccat atccgcccta
       61 acccacgcag tatcgccata gagattagga agttttgcaa tcaattcgat cgcttcctga
      121 ttatctgtcg cgagccccat atgacacata acaaaattca catttggata tttaagtgcc
      181 atctcataaa ccaactgtgg gctggattca taatcattag aagtatggct aactaccggg
      241 agatcatatt tttgagcaag gcgtatatat tcctgaactt tatctgaatt gaaagccatt
      301 tttgaatgat atgggtgaac tttgatgcca taaatgatat ctcgattgct ggcaatcata
      361 tcttcaaaat cttgatcaca actttccaga cgaggcttaa tccacataaa cgcgccaatt
      421 cgctttgaat gttgacgaac aagtctgagc atacgttcat tattatcatg ctgtgtaact
      481 tgatcttcat ccggaatcgg gttctggtca tggtcaactt ctactgcaga tccacaagaa
      541 cataatgcaa aatcaatatt atacttgtcc agagatgcca gaaaaacatc ttctggcata
      601 cggattttca caaagt
//
LOCUS       DAZKHF020000736          615 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2201S31241
            SAMN43267800-rid25106413.denovo.740, whole genome shotgun sequence.
ACCESSION   DAZKHF020000736 DAZKHF020000000
VERSION     DAZKHF020000736.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267800
            Sequence Read Archive: SRR30307263
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 615)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 615)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 615)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350581.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350581.2
            Genome Coverage       :: 151x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:21:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,652
            CDSs (total)                      :: 5,557
            Genes (coding)                    :: 5,320
            CDSs (with protein)               :: 5,320
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 237
            CDSs (without protein)            :: 237
            Pseudo Genes (ambiguous residues) :: 0 of 237
            Pseudo Genes (frameshifted)       :: 102 of 237
            Pseudo Genes (incomplete)         :: 130 of 237
            Pseudo Genes (internal stop)      :: 52 of 237
            Pseudo Genes (multiple problems)  :: 41 of 237
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..615
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267800-rid25106413.denovo.740"
                     /serotype="O 26:H11"
                     /isolate="2201S31241"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-01"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
BASE COUNT          189 a          126 c          115 g          185 t
ORIGIN      
        1 aatacctgta aagctcctca gaagctctat aacgagctaa tctattaccg ctaagtgttt
       61 ccctacctta tatacgttta gctcgcctac ggcttgattt aagcggtaga aacttattta
      121 accttcaact actaaaacca ttctttcttt tttgtttgtt gttggttgtt gtctgtgtct
      181 atgtgctaac atcgctaacg cgtagcacac aaggttattg cactcgtcag agtattgcac
      241 aatagatttt ctcttaaata gattgatgat cgttagattg gcgtaagcct catcaatgga
      301 actattgaag cgcacagcgc atagacacta acaaccattc gcttacgctc aggttattgc
      361 acaagtaaat ttcaatcgtt agatggtagt tagttaatta tcgttctgcg cacagcgcaa
      421 gactattagc catacgcaag ccgtaggcta tttgcacata gtagattaca tattcgttgt
      481 gagcggaaag cgaacataga tcagctaact actaacaaaa aacaaaaaac aaaaactatc
      541 tgttatgaca agcatcagtc taacttgtgc aaggttctgg cgaatgccaa tagattaagg
      601 gatagtcgaa gactc
//
LOCUS       DAZKHF020000737          615 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2201S31241
            SAMN43267800-rid25106413.denovo.741, whole genome shotgun sequence.
ACCESSION   DAZKHF020000737 DAZKHF020000000
VERSION     DAZKHF020000737.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267800
            Sequence Read Archive: SRR30307263
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 615)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 615)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 615)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350581.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350581.2
            Genome Coverage       :: 151x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:21:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,652
            CDSs (total)                      :: 5,557
            Genes (coding)                    :: 5,320
            CDSs (with protein)               :: 5,320
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 237
            CDSs (without protein)            :: 237
            Pseudo Genes (ambiguous residues) :: 0 of 237
            Pseudo Genes (frameshifted)       :: 102 of 237
            Pseudo Genes (incomplete)         :: 130 of 237
            Pseudo Genes (internal stop)      :: 52 of 237
            Pseudo Genes (multiple problems)  :: 41 of 237
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..615
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267800-rid25106413.denovo.741"
                     /serotype="O 26:H11"
                     /isolate="2201S31241"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-01"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            <1..>615
                     /locus_tag="ACHOSC_RS27185"
                     /old_locus_tag="pgaptmp_005437"
     CDS             <1..>615
                     /locus_tag="ACHOSC_RS27185"
                     /old_locus_tag="pgaptmp_005437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="phage tail fiber C-terminal domain-containing
                     protein"
                     /protein_id="HGU4551141.1"
                     /translation="QSAAEAELSRKTAESAAGNAARDATTATEKARESAESAQSAEQS
                     RIAAEEAVNRIPTVVGPPGPKGEPGPAGPQGPKGDKGERGDTGPVGATGERGPAGDAG
                     PAGPQGPKGDRGERGETGLTGDAGPQGPKGDTGAAGPAGPQGPKGETGAAGPVGATGP
                     QGPKGDPGEAQIRFRLGPASIIETNSNGWFPDTDGALITGLTFL"
BASE COUNT          156 a          161 c          232 g           66 t
ORIGIN      
        1 cacaaagtgc agcagaagct gaattgtcaa gaaagacggc agaaagtgca gccggtaatg
       61 cagccaggga tgcaacgacc gcaacagaaa aagcccggga gtcagcagaa agcgcacagt
      121 cagcggaaca aagcaggata gcggcggaag aagccgtaaa ccgaatcccg accgtggtgg
      181 ggcctcccgg gccaaagggg gaaccgggtc ccgcgggtcc tcaggggccg aagggtgata
      241 agggagagcg cggtgacacc ggccctgtcg gggcaaccgg cgaacgggga ccggcaggtg
      301 atgctggtcc ggcaggcccg caggggccga aaggtgacag gggagagcgg ggagagaccg
      361 gtctgacggg agatgcaggt ccacagggtc caaagggaga taccggtgcg gcaggcccgg
      421 caggcccaca gggaccgaaa ggagaaacag gtgcggctgg cccggtgggg gcaaccggac
      481 ctcagggacc gaagggcgac ccgggggagg cacaaatccg ttttcgtctg gggccggcga
      541 gcattattga gacaaacagc aatggctggt tcccggatac agatggcgca ctcatcaccg
      601 gactgacctt tcttg
//
LOCUS       DAZKHF020000738          607 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2201S31241
            SAMN43267800-rid25106413.denovo.742, whole genome shotgun sequence.
ACCESSION   DAZKHF020000738 DAZKHF020000000
VERSION     DAZKHF020000738.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267800
            Sequence Read Archive: SRR30307263
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 607)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 607)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 607)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350581.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350581.2
            Genome Coverage       :: 151x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:21:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,652
            CDSs (total)                      :: 5,557
            Genes (coding)                    :: 5,320
            CDSs (with protein)               :: 5,320
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 237
            CDSs (without protein)            :: 237
            Pseudo Genes (ambiguous residues) :: 0 of 237
            Pseudo Genes (frameshifted)       :: 102 of 237
            Pseudo Genes (incomplete)         :: 130 of 237
            Pseudo Genes (internal stop)      :: 52 of 237
            Pseudo Genes (multiple problems)  :: 41 of 237
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..607
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267800-rid25106413.denovo.742"
                     /serotype="O 26:H11"
                     /isolate="2201S31241"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-01"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            32..>607
                     /gene="espF"
                     /locus_tag="ACHOSC_RS27190"
                     /old_locus_tag="pgaptmp_005438"
     CDS             32..>607
                     /gene="espF"
                     /locus_tag="ACHOSC_RS27190"
                     /old_locus_tag="pgaptmp_005438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system LEE effector EspF"
                     /protein_id="HGU4551142.1"
                     /translation="MLNGISQAVSTLGRHITSAASRVSSAGLSGFSVSPQAVRLNPIR
                     AQSPFSPGTSNINARTTFNVSSPATSFTPSRPAPPPPTSGQASGASRPLPPIAQALKD
                     HLAAYEKSKSLDTSSLKPSRPAPPPPTSGLVSGSSRSLPPIAQALKDHLAAYEKSKSL
                     DTSGLKPSRPAPPPPTSGQASEASRPLPPIAQ"
BASE COUNT          139 a          176 c          143 g          149 t
ORIGIN      
        1 cctgttgatt agatataaaa aggcatgaat tatgcttaat ggaattagtc aagctgtttc
       61 tacactagga cggcatatta ctagtgcggc aagccgggta agctctgcgg ggttatctgg
      121 cttttcagtg tcccctcagg ctgttcgtct gaatccgatt agggctcaat ctcctttttc
      181 tcccggtacg tcgaatatca atgcgagaac gacttttaat gtgagtagcc cggcgacttc
      241 atttactccc tctcgtccgg ccccaccgcc gccgacaagt ggacaggctt ccggggcatc
      301 ccgcccttta ccgcccattg ctcaggcatt aaaagatcat ttagctgcct atgaaaagtc
      361 gaaaagtctt gatacttcaa gccttaagcc aagccgcccg gcaccgccgc cgccgacaag
      421 cgggttggta tctgggtcat cccggtcttt accgcccatt gctcaggcgc taaaagatca
      481 tttggctgcc tatgaaaaat cgaaaagtct tgatacttca ggtcttaagc caagccgccc
      541 ggcaccgccg ccaccgacaa gtggacaggc atccgaggca tcccggccat taccgcctat
      601 tgctcag
//
LOCUS       DAZKHF020000739          605 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2201S31241
            SAMN43267800-rid25106413.denovo.743, whole genome shotgun sequence.
ACCESSION   DAZKHF020000739 DAZKHF020000000
VERSION     DAZKHF020000739.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267800
            Sequence Read Archive: SRR30307263
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 605)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 605)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 605)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350581.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350581.2
            Genome Coverage       :: 151x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:21:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,652
            CDSs (total)                      :: 5,557
            Genes (coding)                    :: 5,320
            CDSs (with protein)               :: 5,320
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 237
            CDSs (without protein)            :: 237
            Pseudo Genes (ambiguous residues) :: 0 of 237
            Pseudo Genes (frameshifted)       :: 102 of 237
            Pseudo Genes (incomplete)         :: 130 of 237
            Pseudo Genes (internal stop)      :: 52 of 237
            Pseudo Genes (multiple problems)  :: 41 of 237
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..605
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267800-rid25106413.denovo.743"
                     /serotype="O 26:H11"
                     /isolate="2201S31241"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-01"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            24..>605
                     /gene="acrS"
                     /locus_tag="ACHOSC_RS27195"
                     /old_locus_tag="pgaptmp_005439"
     CDS             24..>605
                     /gene="acrS"
                     /locus_tag="ACHOSC_RS27195"
                     /old_locus_tag="pgaptmp_005439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux transporter transcriptional
                     repressor AcrS"
                     /protein_id="HGU4551143.1"
                     /translation="MAKRTKAEALKTRQELIETAIAQFAQHGVSKTTLNDIADAANVT
                     RGAIYWHFENKTQLFNEMWLQQPSLRELIQEHLTAGLEHDPFQQLREKLIVGLQYIAK
                     IPRQQALLKILYHKCEFNDEMLAEGVIREKMGFNPQTLREVLQACQQQGCVANNLDLD
                     VVMIIINGAFSGIVQNWLMNMAGYDLYKQAPALV"
BASE COUNT          178 a          129 c          143 g          155 t
ORIGIN      
        1 atttatttcc tgaataatta atcatggcaa aaagaaccaa agccgaagct ctgaagaccc
       61 ggcaagaact gattgaaact gccatcgccc agtttgcgca gcatggcgta agcaagacga
      121 cactcaacga cattgccgac gctgctaacg ttacgcgtgg cgctatctac tggcacttcg
      181 aaaacaagac tcaactgttt aatgagatgt ggttgcaaca gccttcattg cgggagttaa
      241 tccaggaaca cttgacggct ggattagagc atgacccgtt tcaacaattg cgtgaaaaat
      301 tgattgtcgg cttgcaatat attgccaaaa ttccccgcca gcaggcgttg ctgaaaatct
      361 tatatcacaa atgtgaattt aatgatgaga tgctggccga gggagtgata cgcgaaaaga
      421 tgggcttcaa tccgcagact ctccgcgaag tattgcaggc gtgtcagcaa caaggttgtg
      481 tagcaaataa cctcgattta gatgttgtga tgattattat taatggtgcc ttcagcggaa
      541 ttgttcaaaa ctggttaatg aatatggcgg gttatgatct ttataaacaa gcccccgctc
      601 tggtc
//
LOCUS       DAZKHF020000740          605 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2201S31241
            SAMN43267800-rid25106413.denovo.744, whole genome shotgun sequence.
ACCESSION   DAZKHF020000740 DAZKHF020000000
VERSION     DAZKHF020000740.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267800
            Sequence Read Archive: SRR30307263
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 605)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 605)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 605)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350581.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350581.2
            Genome Coverage       :: 151x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:21:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,652
            CDSs (total)                      :: 5,557
            Genes (coding)                    :: 5,320
            CDSs (with protein)               :: 5,320
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 237
            CDSs (without protein)            :: 237
            Pseudo Genes (ambiguous residues) :: 0 of 237
            Pseudo Genes (frameshifted)       :: 102 of 237
            Pseudo Genes (incomplete)         :: 130 of 237
            Pseudo Genes (internal stop)      :: 52 of 237
            Pseudo Genes (multiple problems)  :: 41 of 237
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..605
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267800-rid25106413.denovo.744"
                     /serotype="O 26:H11"
                     /isolate="2201S31241"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-01"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            complement(<1..550)
                     /gene="sctD"
                     /locus_tag="ACHOSC_RS27200"
                     /old_locus_tag="pgaptmp_005440"
     CDS             complement(<1..550)
                     /gene="sctD"
                     /locus_tag="ACHOSC_RS27200"
                     /old_locus_tag="pgaptmp_005440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0030254 - protein secretion by the type III
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system inner membrane ring
                     subunit SctD"
                     /protein_id="HGU4551144.1"
                     /translation="MLSSYKIKLLNGAMRNRELQLPMGNLTIGTEDNDIVYFPLEQGL
                     NQFLLDIREEGVFLLSPVEFWIDGQPTPYEADQSLPVGKVIDIAGCCFIIGDIDHTLP
                     LSDVPERFSAKNRRKKRLILASVIGAAIALSGAIGSYVLLSPKAEPPAFTRADVYQQL
                     KENKLHAITLVWHGKNVALYGRC"
BASE COUNT          178 a          129 c          130 g          168 t
ORIGIN      
        1 cgcaacgccc ataaagcgcg acatttttcc catgccatac cagagtgatt gcatgaagtt
       61 tgttttcttt tagctgctgg tatacatcag ctctggtaaa cgctgggggt tcagcttttg
      121 gggaaagcaa aacgtaactg ccgatggcgc cagacaacgc aatcgcagca ccaatcacgc
      181 tagccaggat aagacgcttt ttacgtcgat ttttggcaga gaacctttct ggcacatcag
      241 acaatggcaa ggtgtggtca atatcaccaa tgatgaagca acatcccgct atatcgatga
      301 cctttccgac aggtaatgac tgatctgctt catagggggt gggctgtcca tcaatccaaa
      361 attcaaccgg agataaaagg aaaacacctt cctcacgtat atcaagtaaa aactgattga
      421 ggccttgttc aaggggaaaa taaacaatat cgttatcttc tgtgcctatc gttaggtttc
      481 ccatcggaag ttgtaattcc cgattacgca tcgcaccatt gagaagtttt attttatatg
      541 aggataacat ttttaaattt tattcatcct ggtggttgaa caatgagaaa aaatatggtg
      601 aactt
//
LOCUS       DAZKHF020000741          600 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2201S31241
            SAMN43267800-rid25106413.denovo.745, whole genome shotgun sequence.
ACCESSION   DAZKHF020000741 DAZKHF020000000
VERSION     DAZKHF020000741.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267800
            Sequence Read Archive: SRR30307263
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 600)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 600)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 600)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350581.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350581.2
            Genome Coverage       :: 151x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:21:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,652
            CDSs (total)                      :: 5,557
            Genes (coding)                    :: 5,320
            CDSs (with protein)               :: 5,320
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 237
            CDSs (without protein)            :: 237
            Pseudo Genes (ambiguous residues) :: 0 of 237
            Pseudo Genes (frameshifted)       :: 102 of 237
            Pseudo Genes (incomplete)         :: 130 of 237
            Pseudo Genes (internal stop)      :: 52 of 237
            Pseudo Genes (multiple problems)  :: 41 of 237
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..600
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267800-rid25106413.denovo.745"
                     /serotype="O 26:H11"
                     /isolate="2201S31241"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-01"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            complement(<1..>600)
                     /locus_tag="ACHOSC_RS27205"
                     /old_locus_tag="pgaptmp_005441"
     CDS             complement(<1..>600)
                     /locus_tag="ACHOSC_RS27205"
                     /old_locus_tag="pgaptmp_005441"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HGU4551145.1"
                     /translation="LETYQSNFQPITRSELNISPEEKIIFYAGRIVPDKGILLLMQSF
                     EKLAAAHKNLKLVVIGDYTEMSKSDKGAYQRNVREIAKRLKDRCIMLGSIPPEKMHCY
                     YPLADLVVIPSQFQEPFCMVAIEAMGAGKPVLVSTRGGMTEFVKENTTGFHLKEPMTA
                     DSISSDILKTLANPELTAVAKQGQDFVFEHYSWDGVTQR"
BASE COUNT          170 a          134 c          105 g          191 t
ORIGIN      
        1 atctttgagt tacgccatcc caactgtagt gttcaaacac aaagtcctga ccttgctttg
       61 caactgctgt taattcagga ttcgctaacg tttttaaaat atcgctgctt atggagtctg
      121 cagtcatagg ttcttttaaa tggaatcccg tagtattttc tttgacaaat tccgtcatcc
      181 ccccacgcgt actcactaaa acaggcttac cagcacccat agcttcaatt gccaccatgc
      241 aaaatggttc ctggaactgg gaaggaatga cgactaaatc tgccagtggg taataacaat
      301 gcatcttttc aggcggtatg cttcctaaca taatacaacg atccttcaaa cgttttgcaa
      361 tttccctgac attgcgttga tatgcacctt tgtcactttt actcatttct gtataatcac
      421 caatcacaac taattttaaa tttttgtgag cagcggccaa tttttcgaat gattgcatca
      481 acagcaatat gcctttgtca gggacgatac gcccagcata aaaaataatt ttctcttcag
      541 gggagatatt aagttcgctt ctagtgatcg gctgaaaatt agattgatag gtttctaaat
//
LOCUS       DAZKHF020000742          579 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2201S31241
            SAMN43267800-rid25106413.denovo.746, whole genome shotgun sequence.
ACCESSION   DAZKHF020000742 DAZKHF020000000
VERSION     DAZKHF020000742.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267800
            Sequence Read Archive: SRR30307263
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 579)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 579)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 579)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350581.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350581.2
            Genome Coverage       :: 151x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:21:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,652
            CDSs (total)                      :: 5,557
            Genes (coding)                    :: 5,320
            CDSs (with protein)               :: 5,320
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 237
            CDSs (without protein)            :: 237
            Pseudo Genes (ambiguous residues) :: 0 of 237
            Pseudo Genes (frameshifted)       :: 102 of 237
            Pseudo Genes (incomplete)         :: 130 of 237
            Pseudo Genes (internal stop)      :: 52 of 237
            Pseudo Genes (multiple problems)  :: 41 of 237
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..579
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267800-rid25106413.denovo.746"
                     /serotype="O 26:H11"
                     /isolate="2201S31241"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-01"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            <1..461
                     /locus_tag="ACHOSC_RS27210"
                     /old_locus_tag="pgaptmp_005442"
     CDS             <1..461
                     /locus_tag="ACHOSC_RS27210"
                     /old_locus_tag="pgaptmp_005442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="GTPase family protein"
                     /protein_id="HGU4551146.1"
                     /translation="ILHRGHQQVLFVVTQADKTEPCHEWDMAGIQPSPAQAQNIREKT
                     EAVFRLFRPVHPVVAVSARTGWELDTLVSALMTALPDHAASPLMTRLQDELRTESVRG
                     QAREQFTGAVDRIFDTAESVCIASVARTVLRAVRDSVVSVARAVWNWIFF"
BASE COUNT          113 a          173 c          163 g          130 t
ORIGIN      
        1 acatcctgca ccggggacat cagcaggtgc tgtttgtggt gacgcaggcc gacaaaacgg
       61 agccctgcca tgaatgggat atggccggta ttcagccttc tcccgcacag gcacagaata
      121 ttcgcgaaaa aacggaggcg gtattccgtc tgttccggcc cgtacatccg gttgtggccg
      181 tatcggcccg caccggctgg gaactggata cgctggtcag tgcgctcatg acagcgcttc
      241 ccgaccatgc cgccagtccc ctgatgaccc gcctgcagga cgagctgcgc acggaatctg
      301 tccggggtca ggcccgtgaa cagtttaccg gtgcggtgga ccggatattt gacacggcgg
      361 agagcgtctg tattgcctct gttgcacgca cggttctgcg ggccgtccgt gactcggtgg
      421 tctctgttgc ccgcgcggta tggaactgga ttttcttctg aacactctgc caggggaaat
      481 gcctgattac attgcgttaa ccaactgaaa acatgccccc ccctcatacc cacctctttc
      541 tcttgcgtga cttccctgct gttaatagaa taaaacgat
//
LOCUS       DAZKHF020000743          579 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2201S31241
            SAMN43267800-rid25106413.denovo.747, whole genome shotgun sequence.
ACCESSION   DAZKHF020000743 DAZKHF020000000
VERSION     DAZKHF020000743.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267800
            Sequence Read Archive: SRR30307263
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 579)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 579)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 579)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350581.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350581.2
            Genome Coverage       :: 151x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:21:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,652
            CDSs (total)                      :: 5,557
            Genes (coding)                    :: 5,320
            CDSs (with protein)               :: 5,320
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 237
            CDSs (without protein)            :: 237
            Pseudo Genes (ambiguous residues) :: 0 of 237
            Pseudo Genes (frameshifted)       :: 102 of 237
            Pseudo Genes (incomplete)         :: 130 of 237
            Pseudo Genes (internal stop)      :: 52 of 237
            Pseudo Genes (multiple problems)  :: 41 of 237
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..579
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267800-rid25106413.denovo.747"
                     /serotype="O 26:H11"
                     /isolate="2201S31241"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-01"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            complement(200..>579)
                     /locus_tag="ACHOSC_RS27215"
                     /old_locus_tag="pgaptmp_005443"
     CDS             complement(200..>579)
                     /locus_tag="ACHOSC_RS27215"
                     /old_locus_tag="pgaptmp_005443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001330961.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4551147.1"
                     /translation="RLREVLNAIFSAPRSGKSRIPKYQQGIFLRNQHRFYLLNHYTES
                     LSVMSLTKLSLNTRMIGFMSDQMLETAPRLTRAVSDETSVYAGAGQNIGQNPFNIIIV
                     ICTKEHIERLELMYQGKSDGFFT"
BASE COUNT          177 a          120 c          101 g          181 t
ORIGIN      
        1 cttgtgttgt caagacattt ggcaacgttc aaacatctgt ttagaaatgc caaattgttg
       61 atctaacaaa taaacctgta taaacagaac accatttaat ctattcccct gtgtaatcca
      121 ctacatcaca cgataaaaac aaaaaacttt taattatatt tcatagcaca caaaagtcaa
      181 cagaagccct ctcgtgctcc tacgtaaaaa atccatcact tttcccctga tacatcaact
      241 caaggcgttc tatgtgttcc ttagtgcata ttacgattat tatgttaaat ggattttggc
      301 ctatattttg acctgcaccc gcataaacac tggtttcatc gcttacagcc cttgtgagac
      361 gtggtgctgt ttctagcatc tgatccgaca taaaaccgat cattctcgtg tttagtgaaa
      421 gtttagtgag agacattaca gaaaggcttt ctgtgtaatg atttaatagg taaaaacgat
      481 gttgattacg caagaaaatt ccttgctgat atttggggat tctggatttt ccagatcggg
      541 gggcgctgaa aatcgcgtta agcacttctc gcaatctag
//
LOCUS       DAZKHF020000744          574 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2201S31241
            SAMN43267800-rid25106413.denovo.748, whole genome shotgun sequence.
ACCESSION   DAZKHF020000744 DAZKHF020000000
VERSION     DAZKHF020000744.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267800
            Sequence Read Archive: SRR30307263
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 574)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 574)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 574)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350581.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350581.2
            Genome Coverage       :: 151x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:21:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,652
            CDSs (total)                      :: 5,557
            Genes (coding)                    :: 5,320
            CDSs (with protein)               :: 5,320
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 237
            CDSs (without protein)            :: 237
            Pseudo Genes (ambiguous residues) :: 0 of 237
            Pseudo Genes (frameshifted)       :: 102 of 237
            Pseudo Genes (incomplete)         :: 130 of 237
            Pseudo Genes (internal stop)      :: 52 of 237
            Pseudo Genes (multiple problems)  :: 41 of 237
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..574
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267800-rid25106413.denovo.748"
                     /serotype="O 26:H11"
                     /isolate="2201S31241"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-01"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            2..>574
                     /locus_tag="ACHOSC_RS27220"
                     /old_locus_tag="pgaptmp_005444"
     CDS             2..>574
                     /locus_tag="ACHOSC_RS27220"
                     /old_locus_tag="pgaptmp_005444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000366210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-mannosidase"
                     /protein_id="HGU4551148.1"
                     /translation="MGQRVYTVVSEMSIDAWITKEPQPFVYRRSGAYQKLTIGSDWGQ
                     LFDCAWMRVYGKRPADKFRHKLVALVDIGGEGLIVDKNGSPICGITNKASSYGVPPDK
                     PGKWVVDLSLVSENDEVEFWIDAACNDLFGYVTNGGIITDVHIATCNQLLKSLYYDVE
                     VLFDWINDGQKFESIHPKGIIPEKIITKRSE"
BASE COUNT          161 a          109 c          134 g          170 t
ORIGIN      
        1 aataggtcag cgtgtttata ctgttgtctc tgaaatgtcg attgatgcat ggataacaaa
       61 agaaccgcag ccttttgttt atcgccgtag cggcgcttac cagaaactca ccattggctc
      121 agactggggt caactgtttg actgtgcctg gatgcgagtt tatggtaaac gtccagcaga
      181 taaatttaga cacaaactcg tcgctctcgt tgatatcggt ggcgaaggtc ttattgtgga
      241 taagaatggt tcacccatct gcggcatcac gaataaagca tcatcttatg gcgtaccgcc
      301 agataaacca ggtaaatggg tggttgacct ttctctcgtt agtgaaaatg acgaggtcga
      361 gttttggatt gatgctgcgt gcaacgattt atttggctat gtcactaatg gagggattat
      421 tactgatgtt catatcgcga catgtaatca actactgaaa tccctttatt atgatgttga
      481 agtgcttttc gactggatta atgatggtca gaagtttgaa tccatacatc ctaaaggtat
      541 tatcccagaa aaaattatca ccaaaagaag cgag
//
LOCUS       DAZKHF020000745          571 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2201S31241
            SAMN43267800-rid25106413.denovo.749, whole genome shotgun sequence.
ACCESSION   DAZKHF020000745 DAZKHF020000000
VERSION     DAZKHF020000745.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267800
            Sequence Read Archive: SRR30307263
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 571)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 571)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 571)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350581.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350581.2
            Genome Coverage       :: 151x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:21:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,652
            CDSs (total)                      :: 5,557
            Genes (coding)                    :: 5,320
            CDSs (with protein)               :: 5,320
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 237
            CDSs (without protein)            :: 237
            Pseudo Genes (ambiguous residues) :: 0 of 237
            Pseudo Genes (frameshifted)       :: 102 of 237
            Pseudo Genes (incomplete)         :: 130 of 237
            Pseudo Genes (internal stop)      :: 52 of 237
            Pseudo Genes (multiple problems)  :: 41 of 237
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..571
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267800-rid25106413.denovo.749"
                     /serotype="O 26:H11"
                     /isolate="2201S31241"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-01"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            <1..226
                     /locus_tag="ACHOSC_RS27225"
                     /old_locus_tag="pgaptmp_005445"
     CDS             <1..226
                     /locus_tag="ACHOSC_RS27225"
                     /old_locus_tag="pgaptmp_005445"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4551149.1"
                     /translation="NRFVFFKISPIRQIAKWNNYSTKTFRYIAITCSPDIRSRYTSTP
                     YSPVLTGSGGGIKRNKEQMAEQATMFVPPA"
BASE COUNT          174 a          140 c          115 g          142 t
ORIGIN      
        1 taatcgtttt gttttcttta agattagtcc tattagacaa atagcaaaat ggaataacta
       61 cagcactaag acgttccgct atatagctat tacctgttcc cctgacataa ggagcagata
      121 tacctcaacc ccctattccc cagtgctgac cgggagcggc ggcggtataa aaaggaacaa
      181 ggaacaaatg gcagaacaag caaccatgtt tgtccccccc gcataaccgt accattcgca
      241 ttaagaaata ttcccatatc tatcccgcaa aagaggaccc gtcatacatc aagtccgttt
      301 tactgggcat cagattatcg ccgtaattag ctcagtgaaa gccgggtaaa cggttagcaa
      361 gctcagtggc tcaaaagtgc atagcgctaa gatgggcctg gttacacctg ccctttgctg
      421 cagatcgtgt tgatcggtgt gatgccgcca cacgtttaag tcaatctgga aaactcacaa
      481 tgcgctcttc tataaagagg actctttgat aaagtaaccc gaaaagggaa agctaactga
      541 tatcaatgta ccaccttagt aatcatccct t
//
LOCUS       DAZKHF020000746          570 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2201S31241
            SAMN43267800-rid25106413.denovo.750, whole genome shotgun sequence.
ACCESSION   DAZKHF020000746 DAZKHF020000000
VERSION     DAZKHF020000746.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267800
            Sequence Read Archive: SRR30307263
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 570)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 570)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 570)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350581.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350581.2
            Genome Coverage       :: 151x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:21:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,652
            CDSs (total)                      :: 5,557
            Genes (coding)                    :: 5,320
            CDSs (with protein)               :: 5,320
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 237
            CDSs (without protein)            :: 237
            Pseudo Genes (ambiguous residues) :: 0 of 237
            Pseudo Genes (frameshifted)       :: 102 of 237
            Pseudo Genes (incomplete)         :: 130 of 237
            Pseudo Genes (internal stop)      :: 52 of 237
            Pseudo Genes (multiple problems)  :: 41 of 237
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..570
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267800-rid25106413.denovo.750"
                     /serotype="O 26:H11"
                     /isolate="2201S31241"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-01"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            <1..>570
                     /locus_tag="ACHOSC_RS27230"
                     /old_locus_tag="pgaptmp_005446"
     CDS             <1..>570
                     /locus_tag="ACHOSC_RS27230"
                     /old_locus_tag="pgaptmp_005446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sialate O-acetylesterase"
                     /protein_id="HGU4551150.1"
                     /translation="PKNVLFAVVWMQGEFDFGGTPANHAAQFGALVDKFRADLADMAG
                     QCVGGSAGGVPWICGDTTYFWKQKNESTYQTVYGSYKNKTEKNIHFVPFMTDENGVNV
                     PTNKPEEDPDIPGIGYYGSKWRDSSATWTSQDRASHFSAWARRGIISDRLATAILRHA
                     GRVALNAGASSTVSEVRPSSPSGAEATGVT"
BASE COUNT          138 a          136 c          179 g          117 t
ORIGIN      
        1 ccgaaaaatg tgctgtttgc cgtggtgtgg atgcaggggg aatttgattt tggcggtacg
       61 ccggcaaatc acgcagcaca gtttggtgcg ctggttgata aattccgtgc agacctggcg
      121 gatatggcag gtcagtgcgt cggtggctct gctggcggtg ttccctggat atgtggagat
      181 acgacgtatt tctggaagca gaagaacgaa tccacgtacc agacggtgta cggcagctat
      241 aaaaacaaaa cggaaaagaa tatccatttc gtaccgttca tgaccgatga gaacggggtg
      301 aatgtgccga cgaacaaacc ggaagaagac ccggacattc cgggtatcgg atattacggt
      361 tcgaaatggc gtgacagctc agccacctgg acgtcacagg acagggcgag ccatttcagc
      421 gcctgggcac gccgtgggat tatttccgac cgtctggcaa cggcgatttt gcgccatgcg
      481 ggaagagtgg cgctaaacgc gggggcatca tcgacagtat cagaggtgcg cccttcatcg
      541 ccttccggtg cagaagccac aggcgtcaca
//
LOCUS       DAZKHF020000747          569 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2201S31241
            SAMN43267800-rid25106413.denovo.751, whole genome shotgun sequence.
ACCESSION   DAZKHF020000747 DAZKHF020000000
VERSION     DAZKHF020000747.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267800
            Sequence Read Archive: SRR30307263
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 569)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 569)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 569)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350581.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350581.2
            Genome Coverage       :: 151x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:21:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,652
            CDSs (total)                      :: 5,557
            Genes (coding)                    :: 5,320
            CDSs (with protein)               :: 5,320
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 237
            CDSs (without protein)            :: 237
            Pseudo Genes (ambiguous residues) :: 0 of 237
            Pseudo Genes (frameshifted)       :: 102 of 237
            Pseudo Genes (incomplete)         :: 130 of 237
            Pseudo Genes (internal stop)      :: 52 of 237
            Pseudo Genes (multiple problems)  :: 41 of 237
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..569
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267800-rid25106413.denovo.751"
                     /serotype="O 26:H11"
                     /isolate="2201S31241"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-01"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            <1..>569
                     /locus_tag="ACHOSC_RS27235"
                     /old_locus_tag="pgaptmp_005447"
     CDS             <1..>569
                     /locus_tag="ACHOSC_RS27235"
                     /old_locus_tag="pgaptmp_005447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HGU4551151.1"
                     /translation="EMLAGKKELYDFTPESIYRAMTIFDILQNKSDIQTLKTECYCLL
                     AECHMSLALHGKSELELAAQKALELLDYVSDITTVDGKILAIMGLITGLSGQAKVSHI
                     LFEQAKIHPTDIASLYYYRALVHFHNEKIEEARICIDKSLQLEPRRRKAVVIKECVDM
                     YVPNPLKNNIKLYYKETESESHRVIIDNI"
BASE COUNT          212 a          103 c          102 g          152 t
ORIGIN      
        1 atgaaatgtt agccggaaag aaagaacttt acgacttcac ccctgaaagc atttacaggg
       61 ccatgactat atttgatata ttacaaaata aaagtgatat acagacgcta aaaacagaat
      121 gttattgcct tctagcggaa tgccatatgt ctttggcact tcatggaaaa agtgaacttg
      181 aacttgctgc tcaaaaagca ttagagcttt tagattatgt atcagacata accactgtcg
      241 atggaaaaat tttagctatt atgggactga taactggtct gtctggacaa gcaaaagtat
      301 ctcatatctt atttgaacag gctaagatac acccaactga tatagcctct ctctactact
      361 atagggcact agtccacttt cataatgaaa aaattgaaga ggcaaggatt tgtatagaca
      421 aatcactaca actcgaaccc agaagacgaa aagcagttgt gataaaagaa tgtgtagata
      481 tgtatgtgcc taacccgctc aaaaacaaca ttaaactcta ctataaagaa actgagagtg
      541 aaagccatcg agttataatt gacaacatt
//
LOCUS       DAZKHF020000748          566 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2201S31241
            SAMN43267800-rid25106413.denovo.752, whole genome shotgun sequence.
ACCESSION   DAZKHF020000748 DAZKHF020000000
VERSION     DAZKHF020000748.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267800
            Sequence Read Archive: SRR30307263
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 566)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 566)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 566)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350581.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350581.2
            Genome Coverage       :: 151x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:21:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,652
            CDSs (total)                      :: 5,557
            Genes (coding)                    :: 5,320
            CDSs (with protein)               :: 5,320
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 237
            CDSs (without protein)            :: 237
            Pseudo Genes (ambiguous residues) :: 0 of 237
            Pseudo Genes (frameshifted)       :: 102 of 237
            Pseudo Genes (incomplete)         :: 130 of 237
            Pseudo Genes (internal stop)      :: 52 of 237
            Pseudo Genes (multiple problems)  :: 41 of 237
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..566
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267800-rid25106413.denovo.752"
                     /serotype="O 26:H11"
                     /isolate="2201S31241"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-01"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            <1..>566
                     /locus_tag="ACHOSC_RS27240"
                     /old_locus_tag="pgaptmp_005448"
     CDS             <1..>566
                     /locus_tag="ACHOSC_RS27240"
                     /old_locus_tag="pgaptmp_005448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="autotransporter domain-containing protein"
                     /protein_id="HGU4551152.1"
                     /translation="NLIHNASGLWADIVALGTRHSMKASTDNNDFRARGWGWLGSLET
                     GLPFSITDNLMLEPQLQYTWQGLSLDDGKDNAGYVKFGHGSAQHVRAGFRLGSHNDMT
                     FGEGTSSRAPLRDSAKHSVSELPVNWWVQPSVIRTFSSRGDMRVGTSTAGSGMTFSPS
                     QNGTSLDLQAGLEARVRENITLGVQAGY"
BASE COUNT          121 a          161 c          168 g          116 t
ORIGIN      
        1 gaatctgata cacaacgcct ccggactgtg ggctgacatt gtggccctgg gaacccgcca
       61 cagcatgaaa gcgtcaacgg acaataacga cttccgcgcc cggggttggg gctggctggg
      121 ttcactggaa accggtctgc ccttcagtat cactgacaac ctgatgctgg agccacaact
      181 gcagtatacc tggcagggac tttccctgga tgacggtaag gacaacgccg gttatgtgaa
      241 gttcgggcat ggcagtgcac aacatgtgcg tgccggtttc cgtctgggca gccacaacga
      301 tatgaccttt ggcgaaggca cctcatcccg tgcccccctg cgtgacagtg caaaacacag
      361 tgtgagtgaa ttaccggtga actggtgggt acagccttct gttatccgca ccttcagctc
      421 ccggggagat atgcgtgtgg ggacttccac tgcaggcagc gggatgacgt tctctccctc
      481 acagaatggc acatcactgg acctgcaggc cggactggaa gcccgtgtcc gggaaaatat
      541 caccctgggc gttcaggccg gttatg
//
LOCUS       DAZKHF020000749          565 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2201S31241
            SAMN43267800-rid25106413.denovo.753, whole genome shotgun sequence.
ACCESSION   DAZKHF020000749 DAZKHF020000000
VERSION     DAZKHF020000749.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267800
            Sequence Read Archive: SRR30307263
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 565)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 565)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 565)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350581.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350581.2
            Genome Coverage       :: 151x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:21:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,652
            CDSs (total)                      :: 5,557
            Genes (coding)                    :: 5,320
            CDSs (with protein)               :: 5,320
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 237
            CDSs (without protein)            :: 237
            Pseudo Genes (ambiguous residues) :: 0 of 237
            Pseudo Genes (frameshifted)       :: 102 of 237
            Pseudo Genes (incomplete)         :: 130 of 237
            Pseudo Genes (internal stop)      :: 52 of 237
            Pseudo Genes (multiple problems)  :: 41 of 237
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..565
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267800-rid25106413.denovo.753"
                     /serotype="O 26:H11"
                     /isolate="2201S31241"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-01"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            <1..>565
                     /locus_tag="ACHOSC_RS27245"
                     /old_locus_tag="pgaptmp_005449"
     CDS             <1..>565
                     /locus_tag="ACHOSC_RS27245"
                     /old_locus_tag="pgaptmp_005449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016243614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HGU4551153.1"
                     /translation="TFIWAICATIFTFSFGFILALALAKKNIRFAKVWRLIFILPYAI
                     PTFVTLLIFRLLLNGIGPVNSFLNSIGMNSVDFLSVPLNAKITVIAVCVWVGAPYFML
                     LIAGALTNISRDLYEASEVDGATKWQQFKEITLPMVLHQVAPTLVMTFAYNFNNFGAI
                     YLLTEGGPTNPEYRFAGHTDIFITWIYK"
BASE COUNT          152 a           99 c          117 g          197 t
ORIGIN      
        1 gacatttatt tgggcaattt gcgcgacgat ttttaccttc agttttggct ttattctggc
       61 tttagcactg gctaaaaaaa atatacgttt cgcaaaagtc tggcgattga tatttatact
      121 gccatatgcc atacccacat ttgtaacgtt acttattttc cgtcttcttt taaatggaat
      181 tggtccggtt aactcgtttc tgaacagtat cggaatgaat tcagttgatt ttctgtcggt
      241 tccattaaat gcaaaaataa cagtaattgc cgtttgtgtt tgggtagggg caccgtattt
      301 tatgctattg atcgcgggtg cattaacaaa catatcccga gatttgtatg aggctagtga
      361 ggtcgatggc gcaactaaat ggcaacagtt taaggaaatt acattgccaa tggtgttaca
      421 tcaggttgcg ccaactctgg taatgacatt tgcatataat ttcaataatt ttggtgcaat
      481 ttatcttctg actgaaggtg gtccgactaa tccggaatac agattcgctg ggcataccga
      541 tatttttatt acctggattt ataaa
//
LOCUS       DAZKHF020000750          564 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2201S31241
            SAMN43267800-rid25106413.denovo.754, whole genome shotgun sequence.
ACCESSION   DAZKHF020000750 DAZKHF020000000
VERSION     DAZKHF020000750.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267800
            Sequence Read Archive: SRR30307263
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 564)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 564)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 564)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350581.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350581.2
            Genome Coverage       :: 151x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:21:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,652
            CDSs (total)                      :: 5,557
            Genes (coding)                    :: 5,320
            CDSs (with protein)               :: 5,320
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 237
            CDSs (without protein)            :: 237
            Pseudo Genes (ambiguous residues) :: 0 of 237
            Pseudo Genes (frameshifted)       :: 102 of 237
            Pseudo Genes (incomplete)         :: 130 of 237
            Pseudo Genes (internal stop)      :: 52 of 237
            Pseudo Genes (multiple problems)  :: 41 of 237
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..564
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267800-rid25106413.denovo.754"
                     /serotype="O 26:H11"
                     /isolate="2201S31241"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-01"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            <1..>564
                     /locus_tag="ACHOSC_RS27250"
                     /old_locus_tag="pgaptmp_005450"
     CDS             <1..>564
                     /locus_tag="ACHOSC_RS27250"
                     /old_locus_tag="pgaptmp_005450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001505174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4551154.1"
                     /translation="LTMSPLLMLNGAVKTGSIVRTIGRGVSYVDIPLALAILGDSWYK
                     AYKHGSSDNPNSAQRFISQEAAFKTTQRVLTQGLSLMSSLSGAIMRSLEKGTPPQMMS
                     LFIVNILNLLFHQNPYEGASASANALKRSTYSHNPDILNTQAIALCVDLQHTKNITMP
                     LFKTKDRISYAFNGQRTSPEDQKQILKE"
BASE COUNT          200 a          118 c           98 g          148 t
ORIGIN      
        1 ctaaccatgt caccactgtt aatgcttaat ggagcagtta aaacaggtag catagtaaga
       61 actataggta ggggagtgag ttatgttgat ataccattgg ccttagctat attaggtgac
      121 tcgtggtata aagcttataa acatggttct tctgataacc caaattctgc tcagagattt
      181 atatcgcaag aagcggcctt taaaaccacc cagcgggtat taacacaagg attaagtctg
      241 atgtcctctt tatcgggagc aatcatgcgc tctcttgaga aaggcacacc accacaaatg
      301 atgtctttat tcatcgtaaa catactaaat ctattatttc atcaaaatcc atatgaaggg
      361 gcatcagcaa gtgctaatgc tttgaaaaga tcaacttact cccataatcc ggacatacta
      421 aatactcagg caatagctct ttgtgttgac ctccagcata caaaaaatat aaccatgcca
      481 cttttcaaaa caaaagacag gatatcttac gcattcaatg gacaaagaac atccccagaa
      541 gaccaaaaac aaatactgaa agaa
//
LOCUS       DAZKHF020000751          562 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2201S31241
            SAMN43267800-rid25106413.denovo.755, whole genome shotgun sequence.
ACCESSION   DAZKHF020000751 DAZKHF020000000
VERSION     DAZKHF020000751.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267800
            Sequence Read Archive: SRR30307263
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 562)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 562)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 562)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350581.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350581.2
            Genome Coverage       :: 151x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:21:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,652
            CDSs (total)                      :: 5,557
            Genes (coding)                    :: 5,320
            CDSs (with protein)               :: 5,320
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 237
            CDSs (without protein)            :: 237
            Pseudo Genes (ambiguous residues) :: 0 of 237
            Pseudo Genes (frameshifted)       :: 102 of 237
            Pseudo Genes (incomplete)         :: 130 of 237
            Pseudo Genes (internal stop)      :: 52 of 237
            Pseudo Genes (multiple problems)  :: 41 of 237
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..562
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267800-rid25106413.denovo.755"
                     /serotype="O 26:H11"
                     /isolate="2201S31241"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-01"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            complement(82..>562)
                     /locus_tag="ACHOSC_RS27255"
                     /old_locus_tag="pgaptmp_005451"
     CDS             complement(82..>562)
                     /locus_tag="ACHOSC_RS27255"
                     /old_locus_tag="pgaptmp_005451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000214982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4551155.1"
                     /translation="IFFDTESNKWYLCVSAACDLVPTQGNDPHHVRLSPHRLIKVLEL
                     FNASQSKALPFAEHSKYIYVMHKNQRKYLSIFEGDKTLPVVDYMVVLNHGTTVDGEEK
                     NIISAVFLSNMDGNVQNVPVRLKLKSQLRTGYAERYQAIASQYSSRIGVDYVSMMLP"
BASE COUNT          195 a          112 c           88 g          167 t
ORIGIN      
        1 acaaaaataa acaacatcat tgtgatgata aatataaaat aggcatggcg aaaaaaaatc
       61 accacgccta aaatataata attatggtag catcattgat acataatcca caccaatcct
      121 tgagctatac tgagacgcta tagcctgata tctttctgca taaccagttc tcagttgaga
      181 tttaagtttg agtcggacag gaacattttg cacgttgcca tccatattac ttaaaaacac
      241 ggcagaaata atattttttt cttcgccatc aactgttgtt ccatgattca acaccaccat
      301 ataatcaaca acaggaagcg ttttatcccc ttcgaaaata gagagatatt ttctttgatt
      361 tttatgcatt acatatatat atttcgaatg ttcagcaaat ggcaatgctt tactctgact
      421 ggcgttaaaa agctccagaa ctttaatgag cctgtgcgga cttaatctta catggtgagg
      481 gtcgttaccc tgagtaggaa ccaaatcaca tgccgcagat acacataaat accatttgtt
      541 cgactctgta tcaaagaaaa ta
//
LOCUS       DAZKHF020000752          560 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2201S31241
            SAMN43267800-rid25106413.denovo.756, whole genome shotgun sequence.
ACCESSION   DAZKHF020000752 DAZKHF020000000
VERSION     DAZKHF020000752.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267800
            Sequence Read Archive: SRR30307263
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 560)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 560)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 560)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350581.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350581.2
            Genome Coverage       :: 151x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:21:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,652
            CDSs (total)                      :: 5,557
            Genes (coding)                    :: 5,320
            CDSs (with protein)               :: 5,320
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 237
            CDSs (without protein)            :: 237
            Pseudo Genes (ambiguous residues) :: 0 of 237
            Pseudo Genes (frameshifted)       :: 102 of 237
            Pseudo Genes (incomplete)         :: 130 of 237
            Pseudo Genes (internal stop)      :: 52 of 237
            Pseudo Genes (multiple problems)  :: 41 of 237
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..560
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267800-rid25106413.denovo.756"
                     /serotype="O 26:H11"
                     /isolate="2201S31241"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-01"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            complement(1..560)
                     /locus_tag="ACHOSC_RS27260"
                     /old_locus_tag="pgaptmp_005452"
                     /pseudo
     CDS             complement(1..560)
                     /locus_tag="ACHOSC_RS27260"
                     /old_locus_tag="pgaptmp_005452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311748.1"
                     /note="frameshifted; incomplete; missing N-terminus and
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="EscU/YscU/HrcU family type III secretion system
                     export apparatus switch protein"
BASE COUNT          167 a          114 c           87 g          192 t
ORIGIN      
        1 ccctcaatga atttatcttc tgaagagagc tgttcatctt gaacaggttg cccagcattc
       61 tccacatctt caagccacag cagaagacgt aatatttcat ctatattttc gaagctaaca
      121 taatcgtagc gacgatgggt agcataattt tttcgtgcta attttttatc tgtgataatt
      181 ggtattccga tttcctttgc atatttcctt acgcctaagg ccacctcatt agttactctt
      241 acagaaatca atggaatagg tgaaagatgt ggcttaaagt atatccctat tgcaatgtga
      301 gtagggttgg aaatcatcaa acgactatta ctgacatcag acttcaattg ctcagaaaga
      361 atttcctgat gcgctctctg cgtttagact taatttctgg atttccttct tgctccttgt
      421 attctctttt aacttcttgt ttatccattt tcatatcttt cataaataaa aaatattcag
      481 cgataaaatc aaaaattaag acaattatca tactgccgag acaatacagt atgagaagga
      541 atagcatctc tccccaatct
//
LOCUS       DAZKHF020000753          555 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2201S31241
            SAMN43267800-rid25106413.denovo.757, whole genome shotgun sequence.
ACCESSION   DAZKHF020000753 DAZKHF020000000
VERSION     DAZKHF020000753.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267800
            Sequence Read Archive: SRR30307263
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 555)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 555)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 555)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350581.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350581.2
            Genome Coverage       :: 151x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:21:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,652
            CDSs (total)                      :: 5,557
            Genes (coding)                    :: 5,320
            CDSs (with protein)               :: 5,320
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 237
            CDSs (without protein)            :: 237
            Pseudo Genes (ambiguous residues) :: 0 of 237
            Pseudo Genes (frameshifted)       :: 102 of 237
            Pseudo Genes (incomplete)         :: 130 of 237
            Pseudo Genes (internal stop)      :: 52 of 237
            Pseudo Genes (multiple problems)  :: 41 of 237
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..555
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267800-rid25106413.denovo.757"
                     /serotype="O 26:H11"
                     /isolate="2201S31241"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-01"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            <1..>555
                     /locus_tag="ACHOSC_RS27265"
                     /old_locus_tag="pgaptmp_005453"
     CDS             <1..>555
                     /locus_tag="ACHOSC_RS27265"
                     /old_locus_tag="pgaptmp_005453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001530200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="RHS repeat-associated core domain-containing
                     protein"
                     /protein_id="HGU4551156.1"
                     /translation="KESGLYYNRHRYYDPLQGRYITPDPIGLRGGWNMYQYPLNPIQV
                     IDPMGLDAIENMTSGGLIYAVSGVPGLIAANSITNSAYQFGYDMDAIVGGAHNGAADA
                     MRHCYLMCRMTKTFGSTIADVIGKNHEAAGDRQGQPAKERIMDLKNNTVGIACGDFSA
                     KCSDACIEKYNTGQLFGLDGIKAD"
BASE COUNT          163 a           96 c          149 g          147 t
ORIGIN      
        1 ataaggagtc ggggctgtac tataaccgtc accggtacta cgatccgttg caggggcggt
       61 atatcacccc ggacccgatt gggttgagag gtggatggaa tatgtatcag tatccgttga
      121 atcccataca agtgatagac ccaatggggt tagatgcgat tgagaatatg acatcaggtg
      181 gactaattta tgccgtatct ggtgtacctg gattgattgc tgcaaacagc attactaaca
      241 gtgcttacca gttcggttat gatatggatg ctattgttgg cggagctcat aatggggccg
      301 ccgatgcaat gagacattgt tacttgatgt gtcgaatgac taagacattt ggatcaacaa
      361 tagctgacgt gataggtaaa aatcatgagg cggcagggga tagacaaggt cagccagcta
      421 aagaaagaat catggatctt aaaaataaca ctgtcggtat tgcttgtggc gatttttctg
      481 ccaaatgtag cgatgcatgt attgaaaaat ataacactgg gcaactcttc gggttagatg
      541 gtataaaagc agata
//
LOCUS       DAZKHF020000754          555 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2201S31241
            SAMN43267800-rid25106413.denovo.758, whole genome shotgun sequence.
ACCESSION   DAZKHF020000754 DAZKHF020000000
VERSION     DAZKHF020000754.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267800
            Sequence Read Archive: SRR30307263
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 555)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 555)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 555)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350581.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350581.2
            Genome Coverage       :: 151x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:21:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,652
            CDSs (total)                      :: 5,557
            Genes (coding)                    :: 5,320
            CDSs (with protein)               :: 5,320
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 237
            CDSs (without protein)            :: 237
            Pseudo Genes (ambiguous residues) :: 0 of 237
            Pseudo Genes (frameshifted)       :: 102 of 237
            Pseudo Genes (incomplete)         :: 130 of 237
            Pseudo Genes (internal stop)      :: 52 of 237
            Pseudo Genes (multiple problems)  :: 41 of 237
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..555
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267800-rid25106413.denovo.758"
                     /serotype="O 26:H11"
                     /isolate="2201S31241"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-01"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            complement(<1..174)
                     /locus_tag="ACHOSC_RS27270"
                     /old_locus_tag="pgaptmp_005454"
     CDS             complement(<1..174)
                     /locus_tag="ACHOSC_RS27270"
                     /old_locus_tag="pgaptmp_005454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1737 domain-containing protein"
                     /protein_id="HGU4551157.1"
                     /translation="MSIKHYDVIRAASPSDLAEKLTHKLKEGWQPYGGPVAITPYTLM
                     QAVAIEGEPQVGPS"
     gene            151..294
                     /locus_tag="ACHOSC_RS27275"
                     /old_locus_tag="pgaptmp_005455"
                     /pseudo
     CDS             151..294
                     /locus_tag="ACHOSC_RS27275"
                     /old_locus_tag="pgaptmp_005455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_303550.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            340..>555
                     /locus_tag="ACHOSC_RS27280"
                     /old_locus_tag="pgaptmp_005456"
     CDS             340..>555
                     /locus_tag="ACHOSC_RS27280"
                     /old_locus_tag="pgaptmp_005456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013009199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4551158.1"
                     /translation="MAKHGAGCLPVNSVSAPESAITPYSFLLIAPPHRGIHHAEVFLI
                     NSKQKNQALCRLFLFITGHSNTTMPQTS"
BASE COUNT          154 a          145 c          131 g          125 t
ORIGIN      
        1 tgaagggccg acctgtggct ctccttcaat agccaccgcc tgcatcagtg tgtacggcgt
       61 aatggcaacc ggtccgccgt atggctgcca gccctctttc agtttgtgtg tcagcttttc
      121 cgcaaggtct gacggcgacg ccgccctgat aacatcgtaa tgtttaatcg acatcgaatt
      181 tctcccgtgt agaggaacag agttaaaaag ccggaagcgg aatcaaatca caggatgacc
      241 atctgccagt ggctggtcgt aaaaaaaagg ccgcgccatg cgcagccgaa aataaaggga
      301 taacgatgat agtttgagaa aaacagaaat aacacttttg tggcaaagca tggtgccggg
      361 tgcctcccgg tgaattcagt atcagcacct gaatccgcga ttaccccata ttccttcttg
      421 ctgattgccc caccgcacag ggggattcac catgcagaag tgtttttaat aaacagcaaa
      481 caaaaaaatc aagcattatg caggctgttt ctttttatca ccggccacag caataccaca
      541 atgccgcaga ccagc
//
LOCUS       DAZKHF020000755          550 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2201S31241
            SAMN43267800-rid25106413.denovo.759, whole genome shotgun sequence.
ACCESSION   DAZKHF020000755 DAZKHF020000000
VERSION     DAZKHF020000755.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267800
            Sequence Read Archive: SRR30307263
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 550)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 550)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 550)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350581.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350581.2
            Genome Coverage       :: 151x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:21:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,652
            CDSs (total)                      :: 5,557
            Genes (coding)                    :: 5,320
            CDSs (with protein)               :: 5,320
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 237
            CDSs (without protein)            :: 237
            Pseudo Genes (ambiguous residues) :: 0 of 237
            Pseudo Genes (frameshifted)       :: 102 of 237
            Pseudo Genes (incomplete)         :: 130 of 237
            Pseudo Genes (internal stop)      :: 52 of 237
            Pseudo Genes (multiple problems)  :: 41 of 237
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..550
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267800-rid25106413.denovo.759"
                     /serotype="O 26:H11"
                     /isolate="2201S31241"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-01"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            complement(<1..271)
                     /locus_tag="ACHOSC_RS27285"
                     /old_locus_tag="pgaptmp_005457"
     CDS             complement(<1..271)
                     /locus_tag="ACHOSC_RS27285"
                     /old_locus_tag="pgaptmp_005457"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4551159.1"
                     /translation="MSSSGQSLALFYHDVGVFISSTRLSISTPYAAFFVTSVSISTML
                     THDVEPVIDTLKSVRRLFSNQVTASCLRLSAGVIEELPVNDGDIMT"
BASE COUNT          162 a          118 c          131 g          139 t
ORIGIN      
        1 atgtcatgat atcgccgtcg ttaacaggta attcttctat gactccagca gacaggcgca
       61 ggcaggaggc ggtcacctga ttactgaaca accttctgac tgacttcagc gtgtcaatca
      121 caggctccac gtcatgcgtc agcatagtgc tgatgctgac ggatgttaca aaaaatgcag
      181 cgtatggcgt tgagatagaa agtctggtgc tggagataaa tacacctaca tcatgataaa
      241 aaagggccag cgactgacct gaagaagaca ctggctaaaa ggctttgcat gtttgtagat
      301 acttattttt cacagacagc aatgatgcct gtcaatatat tattaatatg ctgattgttt
      361 cagttacaga tgctttatta agggaaaatg tcacgatcgg tgcaaaagtg gtttgaaccg
      421 ccccggtttt cctgaaggct gggtcatatc cctccggcag aagcagaaaa agcttattat
      481 gcttccatca gaaacgatga tctggcagcc tgagttcaca gataaaacac tctccaggaa
      541 aaccggggcg
//
LOCUS       DAZKHF020000756          548 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2201S31241
            SAMN43267800-rid25106413.denovo.760, whole genome shotgun sequence.
ACCESSION   DAZKHF020000756 DAZKHF020000000
VERSION     DAZKHF020000756.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267800
            Sequence Read Archive: SRR30307263
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 548)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 548)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 548)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350581.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350581.2
            Genome Coverage       :: 151x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:21:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,652
            CDSs (total)                      :: 5,557
            Genes (coding)                    :: 5,320
            CDSs (with protein)               :: 5,320
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 237
            CDSs (without protein)            :: 237
            Pseudo Genes (ambiguous residues) :: 0 of 237
            Pseudo Genes (frameshifted)       :: 102 of 237
            Pseudo Genes (incomplete)         :: 130 of 237
            Pseudo Genes (internal stop)      :: 52 of 237
            Pseudo Genes (multiple problems)  :: 41 of 237
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..548
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267800-rid25106413.denovo.760"
                     /serotype="O 26:H11"
                     /isolate="2201S31241"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-01"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            complement(<1..518)
                     /locus_tag="ACHOSC_RS27290"
                     /old_locus_tag="pgaptmp_005458"
     CDS             complement(<1..518)
                     /locus_tag="ACHOSC_RS27290"
                     /old_locus_tag="pgaptmp_005458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AIDA-I family autotransporter YfaL"
                     /protein_id="HGU4551160.1"
                     /translation="MRIIFLRKEYLSLLPSMIASLFSANGFAAAIDLCQGYDIKASCH
                     ASRQSLSGITQDWSVADGQWLVFSDMTNNASGGAVFLQQGAEFSLLPENETGMTLFAN
                     NTVTGEYNNGGAIFAKENSTLNLTDVIFSGNVAGGYGGAIYSSGTNDTGVVDLRVTNA
                     MFRNNIANDGKGG"
BASE COUNT          160 a          131 c          111 g          146 t
ORIGIN      
        1 ccacctttgc catcattagc gatgttattg cgaaacatgg cgttagtgac acgtaaatcg
       61 acgacaccag tatcattagt accagaagaa tagattgcgc caccatagcc gcctgcgacg
      121 ttaccagaaa aaataacatc agtaagattc agcgttgagt tttctttagc aaatatggcc
      181 ccgccgttat tatattctcc tgtaacggtg ttattggcaa acagagtcat tccagtttca
      241 ttttctggta atagtgaaaa ttccgctcct tgttgcaaaa atacggctcc tccgctggcg
      301 ttattggtca tatccgaaaa aaccagccat tgcccatcgg cgacactcca gtcctgcgta
      361 atgcctgaaa ggctttgcct gctggcgtga caactcgctt tgatatcata tccctggcat
      421 aaatcaatgg ccgccgcgaa accgttagca gagaaaagag atgcaatcat tgacgggagt
      481 agagataaat actccttgcg tagaaagata atccgcatat tcatctaacc atcattttat
      541 ataagacg
//
LOCUS       DAZKHF020000757          547 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2201S31241
            SAMN43267800-rid25106413.denovo.761, whole genome shotgun sequence.
ACCESSION   DAZKHF020000757 DAZKHF020000000
VERSION     DAZKHF020000757.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267800
            Sequence Read Archive: SRR30307263
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 547)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 547)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 547)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350581.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350581.2
            Genome Coverage       :: 151x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:21:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,652
            CDSs (total)                      :: 5,557
            Genes (coding)                    :: 5,320
            CDSs (with protein)               :: 5,320
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 237
            CDSs (without protein)            :: 237
            Pseudo Genes (ambiguous residues) :: 0 of 237
            Pseudo Genes (frameshifted)       :: 102 of 237
            Pseudo Genes (incomplete)         :: 130 of 237
            Pseudo Genes (internal stop)      :: 52 of 237
            Pseudo Genes (multiple problems)  :: 41 of 237
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..547
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267800-rid25106413.denovo.761"
                     /serotype="O 26:H11"
                     /isolate="2201S31241"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-01"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            complement(<1..504)
                     /locus_tag="ACHOSC_RS27295"
                     /old_locus_tag="pgaptmp_005459"
     CDS             complement(<1..504)
                     /locus_tag="ACHOSC_RS27295"
                     /old_locus_tag="pgaptmp_005459"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HGU4551161.1"
                     /translation="MHYGKVKIFDINHSIVSQYLEIQHKLTRTHLTDVPLYLSLEPNN
                     PALAEALITSQRFSGDTTDMFLMMACLSLFESDERILLFLSGCLSSISAKVRAIIQTD
                     ISASWTLGAIALRLHMSESLLKIKLKNEGHMFSRLLLEERMRVAVNMLCSRHGYGQAV
                     AEKCGYSS"
BASE COUNT          160 a          130 c          104 g          153 t
ORIGIN      
        1 gcttgaataa ccgcattttt ctgctacagc ctgtccatat ccatgccggg aacataacat
       61 attcacagca acacgcatcc gctcttccag caacaagcga ctgaacatgt gcccttcatt
      121 tttcagtttt atctttaaca aactctcact catatgcagg cgtaacgcaa tcgcaccaag
      181 cgtccagctt gctgatatat ctgtctgaat tatcgccctg actttggcac ttatactgga
      241 taaacatcca cttaaaaata ataatatccg ttcatctgat tcaaacagcg acaggcatgc
      301 catcataaga aacatatccg tggtatctcc ggaaaatctc tggctggtaa ttaaagcctc
      361 agccaacgca gggttgttgg gttccagtga cagataaagc ggaacgtcag tcagatgagt
      421 tcttgtcagc ttatgctgaa tttccagata ttgacttact atggaatggt ttatatcgaa
      481 aattttaact ttgccataat gcataaggaa aagcgccctg atgcatttgg tggccagaac
      541 gactgag
//
LOCUS       DAZKHF020000758          545 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2201S31241
            SAMN43267800-rid25106413.denovo.762, whole genome shotgun sequence.
ACCESSION   DAZKHF020000758 DAZKHF020000000
VERSION     DAZKHF020000758.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267800
            Sequence Read Archive: SRR30307263
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 545)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 545)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 545)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350581.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350581.2
            Genome Coverage       :: 151x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:21:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,652
            CDSs (total)                      :: 5,557
            Genes (coding)                    :: 5,320
            CDSs (with protein)               :: 5,320
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 237
            CDSs (without protein)            :: 237
            Pseudo Genes (ambiguous residues) :: 0 of 237
            Pseudo Genes (frameshifted)       :: 102 of 237
            Pseudo Genes (incomplete)         :: 130 of 237
            Pseudo Genes (internal stop)      :: 52 of 237
            Pseudo Genes (multiple problems)  :: 41 of 237
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..545
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267800-rid25106413.denovo.762"
                     /serotype="O 26:H11"
                     /isolate="2201S31241"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-01"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            <1..>545
                     /locus_tag="ACHOSC_RS27300"
                     /old_locus_tag="pgaptmp_005460"
     CDS             <1..>545
                     /locus_tag="ACHOSC_RS27300"
                     /old_locus_tag="pgaptmp_005460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase large subunit"
                     /protein_id="HGU4551162.1"
                     /translation="FEHPPEMVESGAHLLMENLAMVNPNLGYSVDEAFLYREYRKARE
                     AGEEVFRGFMSKHANVEIGLALRSDRWAGADFWEQQGRRVSLDDILQRADVVTVGIDG
                     GGLDDLLGMYVTGRDRETREWLGWGHAWVHETAVVRRKSEASRFQDFVACGDMTIVRR
                     VGDDTAEVAEYVRRIHEAELL"
BASE COUNT          104 a          120 c          201 g          120 t
ORIGIN      
        1 tttgagcatc ctcctgaaat ggtggaaagc ggggctcacc tgctgatgga aaacctcgcc
       61 atggttaacc cgaatctcgg ttattcggtg gatgaggcct ttctgtaccg ggagtaccgt
      121 aaagcccggg aggctggtga ggaagtattt cgtggcttca tgtcaaaaca cgccaatgtg
      181 gaaatcggtc ttgccctgcg ttctgaccgc tgggcgggcg cggatttctg ggagcagcag
      241 ggcaggcgcg tcagcctgga cgatatcctg cagcgcgctg atgtggtgac ggtggggatt
      301 gacggcgggg gcctggatga tctgctggga atgtacgtga ctggccgtga cagggaaacc
      361 cgcgaatggc tgggctgggg ccatgcctgg gtgcatgaaa ccgcggtggt cagacggaag
      421 agtgaggcat cccggtttca ggattttgtg gcctgtggag acatgacgat tgtccgtcgg
      481 gtcggggatg acacggcgga agtggcggag tatgtgcgtc gtattcatga ggctgagtta
      541 ctgga
//
LOCUS       DAZKHF020000759          542 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2201S31241
            SAMN43267800-rid25106413.denovo.763, whole genome shotgun sequence.
ACCESSION   DAZKHF020000759 DAZKHF020000000
VERSION     DAZKHF020000759.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267800
            Sequence Read Archive: SRR30307263
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 542)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 542)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 542)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350581.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350581.2
            Genome Coverage       :: 151x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:21:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,652
            CDSs (total)                      :: 5,557
            Genes (coding)                    :: 5,320
            CDSs (with protein)               :: 5,320
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 237
            CDSs (without protein)            :: 237
            Pseudo Genes (ambiguous residues) :: 0 of 237
            Pseudo Genes (frameshifted)       :: 102 of 237
            Pseudo Genes (incomplete)         :: 130 of 237
            Pseudo Genes (internal stop)      :: 52 of 237
            Pseudo Genes (multiple problems)  :: 41 of 237
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..542
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267800-rid25106413.denovo.763"
                     /serotype="O 26:H11"
                     /isolate="2201S31241"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-01"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            56..271
                     /gene="xisR"
                     /locus_tag="ACHOSC_RS27305"
                     /old_locus_tag="pgaptmp_005461"
     CDS             56..271
                     /gene="xisR"
                     /locus_tag="ACHOSC_RS27305"
                     /old_locus_tag="pgaptmp_005461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309957.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excisionase XisR"
                     /protein_id="HGU4551163.1"
                     /translation="MAQVIFNEEWMVEYGLMLRTGLGARQIEAYRQNCWVEGFHFKRV
                     SPLGKPDSKRGIIWYNYPKINQFIKDS"
     gene            273..>542
                     /locus_tag="ACHOSC_RS27310"
                     /old_locus_tag="pgaptmp_005462"
     CDS             273..>542
                     /locus_tag="ACHOSC_RS27310"
                     /old_locus_tag="pgaptmp_005462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3596 domain-containing protein"
                     /protein_id="HGU4551164.1"
                     /translation="MSKLPTGVEIRGRYIRIWFMFRGKRCRETLKGWEITNSNIKKAG
                     NLRALIVHEINSGEFEYLRRFPQSSTGAKMVTTRVIKTFGELCDIW"
BASE COUNT          172 a           99 c          136 g          135 t
ORIGIN      
        1 atcaacatcg ggcgtcaatt tcagtctaac attggcgcct gccagaggtg atgcgatggc
       61 acaagtaatc tttaatgaag agtggatggt tgaatacggc ctgatgcttc gcactggtct
      121 gggggccaga caaattgaag cataccgcca gaactgttgg gtggagggct tccacttcaa
      181 acgagtatct cctttaggta agccagacag caaacgaggg attatctggt acaactatcc
      241 aaagataaat cagtttatca aagactcatg atatgtctaa attaccaaca ggtgtcgaga
      301 ttagaggtag atacattcgc atctggttca tgtttcgagg aaaacgatgt cgggaaacat
      361 taaaaggctg ggagattaca aacagtaata ttaaaaaggc cggaaattta agagcgctga
      421 tagttcatga aataaactcc ggtgaatttg agtatttaag acgttttccc cagtccagca
      481 ctggggcaaa aatggtgaca acgagagtca taaaaacgtt cggagagctt tgtgatatct
      541 gg
//
LOCUS       DAZKHF020000760          542 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2201S31241
            SAMN43267800-rid25106413.denovo.764, whole genome shotgun sequence.
ACCESSION   DAZKHF020000760 DAZKHF020000000
VERSION     DAZKHF020000760.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267800
            Sequence Read Archive: SRR30307263
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 542)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 542)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 542)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350581.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350581.2
            Genome Coverage       :: 151x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:21:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,652
            CDSs (total)                      :: 5,557
            Genes (coding)                    :: 5,320
            CDSs (with protein)               :: 5,320
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 237
            CDSs (without protein)            :: 237
            Pseudo Genes (ambiguous residues) :: 0 of 237
            Pseudo Genes (frameshifted)       :: 102 of 237
            Pseudo Genes (incomplete)         :: 130 of 237
            Pseudo Genes (internal stop)      :: 52 of 237
            Pseudo Genes (multiple problems)  :: 41 of 237
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..542
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267800-rid25106413.denovo.764"
                     /serotype="O 26:H11"
                     /isolate="2201S31241"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-01"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            <1..>542
                     /locus_tag="ACHOSC_RS27315"
                     /old_locus_tag="pgaptmp_005463"
     CDS             <1..>542
                     /locus_tag="ACHOSC_RS27315"
                     /old_locus_tag="pgaptmp_005463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HGU4551165.1"
                     /translation="ELVEDPEAILRYGRNLLKMDAFGCTSRGQAHRAGLWVIKTGLLE
                     TQTVDFTLGSQGLRHTPGDIIEICDNDYAGTLTGGRVLSIDAASRTLTLDREVTLPET
                     GTSTVNLINGSGKPVSVDITAHPAPDRIQVSTLPDGVATYGVWGLSLPSLRRRLFRCV
                     SIRENTDGTFAITAVQHVPE"
BASE COUNT          112 a          164 c          172 g           94 t
ORIGIN      
        1 gaactggtgg aagacccgga agccatactg cgctacggac gcaacctgct gaagatggac
       61 gcgttcggct gtaccagccg cggtcaggcc caccgtgccg gactgtgggt gataaagacc
      121 ggactgctgg aaacgcagac ggtggatttc acgctcgggt ctcaggggct gcggcacaca
      181 cccggtgaca tcattgaaat ctgtgataac gactatgccg ggaccctgac cggcggacgt
      241 gtcctgtcca ttgatgccgc cagccgcacc ctgacgctgg accgtgaggt gacactgccg
      301 gagacaggta catcgacggt gaacctgatt aacggcagcg gtaagccggt gagtgtggac
      361 atcaccgcac accccgcgcc ggaccggata caggtcagta ccctgccgga tggcgtggcg
      421 acatacggtg tatggggact ctccctgccg tcactgcgtc gtcgcctgtt ccgctgtgtt
      481 tccatccggg aaaacacgga cggcaccttt gccatcacgg cggtgcagca cgtaccggaa
      541 aa
//
LOCUS       DAZKHF020000761          541 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2201S31241
            SAMN43267800-rid25106413.denovo.765, whole genome shotgun sequence.
ACCESSION   DAZKHF020000761 DAZKHF020000000
VERSION     DAZKHF020000761.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267800
            Sequence Read Archive: SRR30307263
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 541)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 541)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 541)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350581.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350581.2
            Genome Coverage       :: 151x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:21:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,652
            CDSs (total)                      :: 5,557
            Genes (coding)                    :: 5,320
            CDSs (with protein)               :: 5,320
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 237
            CDSs (without protein)            :: 237
            Pseudo Genes (ambiguous residues) :: 0 of 237
            Pseudo Genes (frameshifted)       :: 102 of 237
            Pseudo Genes (incomplete)         :: 130 of 237
            Pseudo Genes (internal stop)      :: 52 of 237
            Pseudo Genes (multiple problems)  :: 41 of 237
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..541
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267800-rid25106413.denovo.765"
                     /serotype="O 26:H11"
                     /isolate="2201S31241"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-01"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            <1..>541
                     /locus_tag="ACHOSC_RS27320"
                     /old_locus_tag="pgaptmp_005464"
     CDS             <1..>541
                     /locus_tag="ACHOSC_RS27320"
                     /old_locus_tag="pgaptmp_005464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="exonuclease"
                     /protein_id="HGU4551166.1"
                     /translation="DSWDAEYMQENRWDEESASFVPVETESDPINVTFDKLAPEVQNA
                     VMVKFDTCENITVDMVISAQELLQEDMATFDGHIVEALMKMPEVNAMYPELKLHAIGW
                     VKHKCKPGAKWPEIQAEMRIWKKRREGERKETGKYTSVVDLARARANQQHTENSTGKI
                     SPVIAAIHREYKQTWKTLD"
BASE COUNT          166 a          121 c          140 g          114 t
ORIGIN      
        1 tcgattcctg ggatgctgaa tatatgcagg aaaaccgctg ggatgaggag tctgcttcct
       61 ttgtcccggt tgagactgaa tcagatccga taaacgtcac ttttgacaag ctggcccctg
      121 aagtacagaa cgctgtcatg gttaagttcg acacatgtga aaacatcacc gttgatatgg
      181 tgattagcgc gcaggaactg ttgcaggaag acatggcaac attcgacgga catatcgttg
      241 aagcgttgat gaaaatgcca gaagttaacg ccatgtatcc ggagcttaag ctgcatgcca
      301 tcgggtgggt taagcataaa tgtaagccag gtgccaaatg gcccgaaatt caggcagaga
      361 tgcgcatctg gaaaaaacgt cgcgaaggtg aacgcaagga aaccggaaaa tacacgtctg
      421 ttgttgatct cgcccgcgcc agagccaatc aacagcacac tgaaaattca acaggaaaaa
      481 tcagcccggt cattgctgcc attcatcgcg aatacaagca gacatggaaa acactggatg
      541 a
//
LOCUS       DAZKHF020000762          539 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2201S31241
            SAMN43267800-rid25106413.denovo.766, whole genome shotgun sequence.
ACCESSION   DAZKHF020000762 DAZKHF020000000
VERSION     DAZKHF020000762.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267800
            Sequence Read Archive: SRR30307263
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 539)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 539)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 539)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350581.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350581.2
            Genome Coverage       :: 151x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:21:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,652
            CDSs (total)                      :: 5,557
            Genes (coding)                    :: 5,320
            CDSs (with protein)               :: 5,320
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 237
            CDSs (without protein)            :: 237
            Pseudo Genes (ambiguous residues) :: 0 of 237
            Pseudo Genes (frameshifted)       :: 102 of 237
            Pseudo Genes (incomplete)         :: 130 of 237
            Pseudo Genes (internal stop)      :: 52 of 237
            Pseudo Genes (multiple problems)  :: 41 of 237
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..539
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267800-rid25106413.denovo.766"
                     /serotype="O 26:H11"
                     /isolate="2201S31241"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-01"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            complement(125..334)
                     /locus_tag="ACHOSC_RS27325"
                     /old_locus_tag="pgaptmp_005465"
     CDS             complement(125..334)
                     /locus_tag="ACHOSC_RS27325"
                     /old_locus_tag="pgaptmp_005465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309578.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4035 domain-containing protein"
                     /protein_id="HGU4551167.1"
                     /translation="MWMEFDRVSPLGDERGDIRNAQIVKAVFGAQGMNVSLEDAMLCW
                     GEDEDKVESDPFAALEDALSFAAMS"
     gene            complement(430..>539)
                     /locus_tag="ACHOSC_RS27330"
                     /old_locus_tag="pgaptmp_005466"
     CDS             complement(430..>539)
                     /locus_tag="ACHOSC_RS27330"
                     /old_locus_tag="pgaptmp_005466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="phage tail assembly chaperone"
                     /protein_id="HGU4551168.1"
                     /translation="PDDREQVLAVYGPVHARLLRQALELIADAESARKK"
BASE COUNT          120 a          178 c          125 g          116 t
ORIGIN      
        1 gcacgctgga gctccgactg gaatgactgt gaatttgccg aaattttgat aatcagttca
       61 cgtaacgtcg ccattcacct ttctccgggc aaaaaaacct gccacagcag gttttcatca
      121 ttatttatga cattgctgca aagctcagcg cgtcttccag cgccgcaaac ggatctgact
      181 ccactttatc ctcatcctcg ccccagcaga gcatggcgtc ctccagtgaa acattcatcc
      241 cctgcgcccc gaaaaccgct ttcacgatct gcgcattacg gatatccccg cgctcatcgc
      301 ccagcgggga caccctgtcg aactccatcc acatcatcgc ctcgctcgca ctcagactgt
      361 ggcccagttc ggataaggtg cgccccagac ggagcgcaag tcgcatcaga aagagaattt
      421 ccgggcgggc tacttttttc tggccgactc tgcatcagcg atcagttcca gtgcctgacg
      481 cagcaaccgg gcatgtaccg gaccatagac ggccagcacc tgctcacggt cgtccggag
//
LOCUS       DAZKHF020000763          539 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2201S31241
            SAMN43267800-rid25106413.denovo.767, whole genome shotgun sequence.
ACCESSION   DAZKHF020000763 DAZKHF020000000
VERSION     DAZKHF020000763.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267800
            Sequence Read Archive: SRR30307263
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 539)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 539)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 539)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350581.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350581.2
            Genome Coverage       :: 151x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:21:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,652
            CDSs (total)                      :: 5,557
            Genes (coding)                    :: 5,320
            CDSs (with protein)               :: 5,320
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 237
            CDSs (without protein)            :: 237
            Pseudo Genes (ambiguous residues) :: 0 of 237
            Pseudo Genes (frameshifted)       :: 102 of 237
            Pseudo Genes (incomplete)         :: 130 of 237
            Pseudo Genes (internal stop)      :: 52 of 237
            Pseudo Genes (multiple problems)  :: 41 of 237
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..539
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267800-rid25106413.denovo.767"
                     /serotype="O 26:H11"
                     /isolate="2201S31241"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-01"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            <1..85
                     /locus_tag="ACHOSC_RS27335"
                     /old_locus_tag="pgaptmp_005467"
     CDS             <1..85
                     /locus_tag="ACHOSC_RS27335"
                     /old_locus_tag="pgaptmp_005467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707066.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="cyclic diguanylate phosphodiesterase (EAL)
                     domain protein"
                     /protein_id="HGU4551169.1"
                     /translation="YYFYKPVTYIDLVKIILSKPKVKVVVE"
     gene            217..435
                     /gene="ymgF"
                     /locus_tag="ACHOSC_RS27340"
                     /old_locus_tag="pgaptmp_005468"
     CDS             217..435
                     /gene="ymgF"
                     /locus_tag="ACHOSC_RS27340"
                     /old_locus_tag="pgaptmp_005468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division-associated protein YmgF"
                     /protein_id="HGU4551170.1"
                     /translation="MNNSNNLDYFTLYIIFSIAFMLITLLVILIAKPSTGLGEVLVTI
                     NLLNALVWLAINLVNRLRERLVSHRDQQ"
BASE COUNT          144 a           98 c          114 g          183 t
ORIGIN      
        1 ttattatttc tataaacctg ttacatacat cgacctggtc aaaattatcc tttctaaacc
       61 gaaggtgaag gttgtggttg agtgaaaatt gatcagtaag gccatagtgc ggtgtaatta
      121 tagacagcta attagctcgt tgcctcttgt tactattgtt cattattttg tttgctataa
      181 ttgtttgaaa gttttgacag gattgccatt agtagcatga acaatagtaa taatctggat
      241 tatttcactc tctatatcat cttttccatt gcatttatgc tgatcaccct cctggtcatc
      301 cttattgcaa aacccagtac cgggctggga gaagtgcttg tgacgataaa tttgcttaat
      361 gcccttgttt ggctggcgat caatctggtt aatcgattaa gggaaagact cgtcagccac
      421 agggatcagc aataatcttt cagtttctca ctgtcagtat gcggctgaat gggttgctgg
      481 cagtgaatgc ctggatcatt gaagtaaagg aattattgcg caaatagttg tcaaccctg
//
LOCUS       DAZKHF020000764          537 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2201S31241
            SAMN43267800-rid25106413.denovo.768, whole genome shotgun sequence.
ACCESSION   DAZKHF020000764 DAZKHF020000000
VERSION     DAZKHF020000764.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267800
            Sequence Read Archive: SRR30307263
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 537)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 537)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 537)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350581.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350581.2
            Genome Coverage       :: 151x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:21:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,652
            CDSs (total)                      :: 5,557
            Genes (coding)                    :: 5,320
            CDSs (with protein)               :: 5,320
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 237
            CDSs (without protein)            :: 237
            Pseudo Genes (ambiguous residues) :: 0 of 237
            Pseudo Genes (frameshifted)       :: 102 of 237
            Pseudo Genes (incomplete)         :: 130 of 237
            Pseudo Genes (internal stop)      :: 52 of 237
            Pseudo Genes (multiple problems)  :: 41 of 237
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..537
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267800-rid25106413.denovo.768"
                     /serotype="O 26:H11"
                     /isolate="2201S31241"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-01"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            <1..99
                     /locus_tag="ACHOSC_RS27345"
                     /old_locus_tag="pgaptmp_005469"
     CDS             <1..99
                     /locus_tag="ACHOSC_RS27345"
                     /old_locus_tag="pgaptmp_005469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000031374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase"
                     /protein_id="HGU4551171.1"
                     /translation="AKAWKMLEGTPEQHKAQDVYNIRRDELEGAAA"
     gene            99..482
                     /locus_tag="ACHOSC_RS27350"
                     /old_locus_tag="pgaptmp_005470"
     CDS             99..482
                     /locus_tag="ACHOSC_RS27350"
                     /old_locus_tag="pgaptmp_005470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000951334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4551172.1"
                     /translation="MAHSITVRLNKPAREFQTGENIGFNIRAGVQYYDRQTKKKEWTN
                     YSAVVFAKPGAQADYYRSVLVEGGIVEITGENIRVDVYQGQNGQSITLELLNAKIGFA
                     ASGNGPQQQSSNQQNTPVYDDSIPF"
BASE COUNT          171 a          113 c          136 g          117 t
ORIGIN      
        1 gccaaagcgt ggaagatgct cgaaggcaca ccggagcagc acaaagcgca ggacgtttac
       61 aacatcagac gagacgaatt agaaggagct gctgcttaat ggcacattcg attactgtaa
      121 gactaaacaa gcccgcaaga gagtttcaga ccggggaaaa tatcggattc aacatccgtg
      181 ctggcgttca gtattacgat cgccagacaa aaaagaaaga atggacaaac tacagcgccg
      241 ttgtatttgc caagccggga gcgcaagcgg attactaccg tagtgttctt gttgaaggtg
      301 gcattgtgga aattaccgga gaaaacatca gggttgatgt ttatcagggg caaaatggtc
      361 aatcaatcac tcttgaatta ctgaatgcaa agattggatt tgcagcttca ggaaatggcc
      421 cgcagcagca aagtagtaac cagcagaaca ctcctgtata cgacgattcc atcccattct
      481 gatttagaaa aataaggatt taattatgcc agcgcctctg tatggtgcgg atgacgc
//
LOCUS       DAZKHF020000765          533 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2201S31241
            SAMN43267800-rid25106413.denovo.769, whole genome shotgun sequence.
ACCESSION   DAZKHF020000765 DAZKHF020000000
VERSION     DAZKHF020000765.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267800
            Sequence Read Archive: SRR30307263
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 533)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 533)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 533)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350581.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350581.2
            Genome Coverage       :: 151x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:21:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,652
            CDSs (total)                      :: 5,557
            Genes (coding)                    :: 5,320
            CDSs (with protein)               :: 5,320
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 237
            CDSs (without protein)            :: 237
            Pseudo Genes (ambiguous residues) :: 0 of 237
            Pseudo Genes (frameshifted)       :: 102 of 237
            Pseudo Genes (incomplete)         :: 130 of 237
            Pseudo Genes (internal stop)      :: 52 of 237
            Pseudo Genes (multiple problems)  :: 41 of 237
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..533
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267800-rid25106413.denovo.769"
                     /serotype="O 26:H11"
                     /isolate="2201S31241"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-01"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            complement(63..155)
                     /locus_tag="ACHOSC_RS27355"
                     /old_locus_tag="pgaptmp_005471"
                     /pseudo
     CDS             complement(63..155)
                     /locus_tag="ACHOSC_RS27355"
                     /old_locus_tag="pgaptmp_005471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000219338.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="regulator"
     gene            complement(188..>533)
                     /locus_tag="ACHOSC_RS27360"
                     /old_locus_tag="pgaptmp_005472"
     CDS             complement(188..>533)
                     /locus_tag="ACHOSC_RS27360"
                     /old_locus_tag="pgaptmp_005472"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4551173.1"
                     /translation="LSMLMVKKTIYVNPDRGQNRKVSDRGLTSRDRRRIARWEKRIAY
                     ALKNGVTPGFNAIDDGPEYKINEDPMDKVDKALATPFPRDVEKIEDEKYEDVMHRVVN
                     HAHQRNPNKKWS"
BASE COUNT          132 a          120 c           87 g          194 t
ORIGIN      
        1 tccatcatgt tcagtttctg attcaggaaa aggtacgctc catgattttg tcatatctca
       61 cctcaaataa gtggtttgct gcctaatttc attttctggc gaccaacaca agtcacaccc
      121 atttcactgc gtggcttgct gtaataaatt aggttagttc agccaataaa aaacccaccg
      181 aagtgggcta tgaccatttt ttatttggat ttcgttggtg agcgtgatta acaactctgt
      241 gcattacatc ctcatatttt tcatcttcaa ttttttcgac atcgcgagga aatggtgttg
      301 ctaatgcttt gtcaactttg tccattgggt cttcattaat cttatattca ggaccgtcat
      361 ctatagcatt aaatccaggt gttacaccgt tttttaatgc atatgctatc ctcttttccc
      421 atctcgctat tctcctcctg tctcgagatg taagacctct atcagatact tttctgtttt
      481 gtccgcggtc aggattaaca taaatagtct ttttcaccat aagcatactc aat
//
LOCUS       DAZKHF020000766          530 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2201S31241
            SAMN43267800-rid25106413.denovo.770, whole genome shotgun sequence.
ACCESSION   DAZKHF020000766 DAZKHF020000000
VERSION     DAZKHF020000766.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267800
            Sequence Read Archive: SRR30307263
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 530)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 530)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 530)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350581.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350581.2
            Genome Coverage       :: 151x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:21:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,652
            CDSs (total)                      :: 5,557
            Genes (coding)                    :: 5,320
            CDSs (with protein)               :: 5,320
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 237
            CDSs (without protein)            :: 237
            Pseudo Genes (ambiguous residues) :: 0 of 237
            Pseudo Genes (frameshifted)       :: 102 of 237
            Pseudo Genes (incomplete)         :: 130 of 237
            Pseudo Genes (internal stop)      :: 52 of 237
            Pseudo Genes (multiple problems)  :: 41 of 237
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..530
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267800-rid25106413.denovo.770"
                     /serotype="O 26:H11"
                     /isolate="2201S31241"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-01"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            16..>530
                     /locus_tag="ACHOSC_RS27365"
                     /old_locus_tag="pgaptmp_005473"
     CDS             16..>530
                     /locus_tag="ACHOSC_RS27365"
                     /old_locus_tag="pgaptmp_005473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415458.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HGU4551174.1"
                     /translation="MKKSVLTAFITVVCATSSVMAADDNAITDGKVTFNGKVIAPACT
                     LVAAKKDSVVTLPNVSATKLQTNGAVSGVKTDVPIALEGCDVTVTKNATFTFSGTADG
                     VQPTAFANQATTDAATNVALQMYLPDGSTSVTPGTETSNIQLADSAEQTVTFKVDYIA
                     TGKATSGNVNAV"
BASE COUNT          152 a          108 c          130 g          140 t
ORIGIN      
        1 atcaggatga tgacgatgaa aaaaagtgta ttgacggcgt ttataactgt ggtatgtgca
       61 acgtccagcg ttatggcagc tgatgataat gctatcacgg atggcaaagt aacatttaat
      121 ggtaaagtta ttgctccagc ttgtaccctg gtagctgcga agaaagattc agtagtcaca
      181 ttgccaaatg tcagcgccac caagttgcaa actaatggag cagtctctgg cgttaaaact
      241 gatgtaccaa ttgccttaga aggctgtgat gttactgtaa ctaaaaatgc gacgtttacc
      301 tttagtggta cggctgacgg cgtgcaacct acagcatttg ccaaccaggc taccaccgat
      361 gcagcgacga atgttgctct acaaatgtat ctgccagatg gttctacatc ggtaactccc
      421 ggtaccgaaa cgtcgaatat tcagttagcg gattctgctg agcaaacagt gacctttaaa
      481 gttgattata tcgctacggg gaaagcgact tctggtaatg tgaatgcggt
//
LOCUS       DAZKHF020000767          528 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2201S31241
            SAMN43267800-rid25106413.denovo.771, whole genome shotgun sequence.
ACCESSION   DAZKHF020000767 DAZKHF020000000
VERSION     DAZKHF020000767.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267800
            Sequence Read Archive: SRR30307263
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 528)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 528)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 528)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350581.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350581.2
            Genome Coverage       :: 151x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:21:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,652
            CDSs (total)                      :: 5,557
            Genes (coding)                    :: 5,320
            CDSs (with protein)               :: 5,320
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 237
            CDSs (without protein)            :: 237
            Pseudo Genes (ambiguous residues) :: 0 of 237
            Pseudo Genes (frameshifted)       :: 102 of 237
            Pseudo Genes (incomplete)         :: 130 of 237
            Pseudo Genes (internal stop)      :: 52 of 237
            Pseudo Genes (multiple problems)  :: 41 of 237
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..528
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267800-rid25106413.denovo.771"
                     /serotype="O 26:H11"
                     /isolate="2201S31241"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-01"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            <1..118
                     /locus_tag="ACHOSC_RS27370"
                     /old_locus_tag="pgaptmp_005474"
     CDS             <1..118
                     /locus_tag="ACHOSC_RS27370"
                     /old_locus_tag="pgaptmp_005474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005576 - extracellular region [Evidence
                     IEA];
                     GO_process: GO:0019836 - hemolysis by symbiont of host
                     erythrocytes [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="Shiga-like toxin beta subunit"
                     /protein_id="HGU4551175.1"
                     /translation="NRWNLQSLLLSAQITGMTVTIKTNACHNGGGFSEVIFR"
BASE COUNT          140 a          108 c          134 g          146 t
ORIGIN      
        1 caacagatgg aatcttcagt ctcttcttct cagtgcgcaa attacgggga tgactgtaac
       61 cattaaaact aatgcctgtc ataatggagg gggattcagc gaagttattt ttcgttgact
      121 cagaatagct cagtgaaaat agcaggcgga gattcataaa tgttaaatac atctcaattc
      181 agtcagttgt tgccggtctg ataatagatg tgttagaaaa tttctgcatg gtgaatcccc
      241 ctgtgcggag gggcgactgg tgaacggtat gatctctttg atgatcgtaa gcgagaatac
      301 gcgggtttgg tggcaccagg ccgaactcac cgggaggcac ccggcatcat gctgtataca
      361 gagattaggc atatatccag gctcctcatc gcaggagcct ttttacatgc aaaaaaaagc
      421 ccgagtgggt tcgggcaaca gcatgagata cttgcattgt catttttatc gtgcggattt
      481 taaccaggat tcataaggct gcgcaactgc gcggcctttt tcgtattt
//
LOCUS       DAZKHF020000768          526 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2201S31241
            SAMN43267800-rid25106413.denovo.772, whole genome shotgun sequence.
ACCESSION   DAZKHF020000768 DAZKHF020000000
VERSION     DAZKHF020000768.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267800
            Sequence Read Archive: SRR30307263
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 526)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 526)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 526)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350581.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350581.2
            Genome Coverage       :: 151x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:21:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,652
            CDSs (total)                      :: 5,557
            Genes (coding)                    :: 5,320
            CDSs (with protein)               :: 5,320
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 237
            CDSs (without protein)            :: 237
            Pseudo Genes (ambiguous residues) :: 0 of 237
            Pseudo Genes (frameshifted)       :: 102 of 237
            Pseudo Genes (incomplete)         :: 130 of 237
            Pseudo Genes (internal stop)      :: 52 of 237
            Pseudo Genes (multiple problems)  :: 41 of 237
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..526
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267800-rid25106413.denovo.772"
                     /serotype="O 26:H11"
                     /isolate="2201S31241"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-01"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            <1..>526
                     /locus_tag="ACHOSC_RS27375"
                     /old_locus_tag="pgaptmp_005475"
     CDS             <1..>526
                     /locus_tag="ACHOSC_RS27375"
                     /old_locus_tag="pgaptmp_005475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016830 - carbon-carbon lyase activity
                     [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0019752 - carboxylic acid metabolic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="pyridoxal-dependent decarboxylase"
                     /protein_id="HGU4551176.1"
                     /translation="PLHEMRDDVAFQIINDELYLDGNARQNLATFCQTWDDENVHKLM
                     DLSINKNWIDKEEYPQSAAIDLRCVNMVADLWHAPAPKNGQAVGTNTIGSSEACMLGG
                     MAMKWRWRKRMEAAGKPTDKPNLVCGPVQICWHKFARYWDVELREIPMRPGQLFMDPK
                     RMIEACDENTIGVVP"
BASE COUNT          135 a          135 c          141 g          115 t
ORIGIN      
        1 ttccgctgca cgaaatgcgc gacgatgtcg cattccagat tatcaatgac gaattatatc
       61 ttgatggcaa cgctcgtcag aacctggcca ctttctgcca gacctgggac gacgaaaatg
      121 tccacaaatt gatggattta tccattaaca aaaactggat cgacaaagaa gaatatccgc
      181 aatccgcagc catcgacctg cgttgcgtaa atatggttgc cgatctgtgg catgcgcctg
      241 cgccgaaaaa tggtcaggcc gttggcacca acaccattgg ttcttccgag gcctgtatgc
      301 tcggcgggat ggcgatgaaa tggcgttggc gcaagcgtat ggaagctgca ggcaaaccaa
      361 cggataaacc aaacctggtg tgcggtccgg tacaaatctg ctggcataaa ttcgcccgct
      421 actgggatgt ggagctgcgt gagatcccta tgcgccccgg tcagttgttt atggacccga
      481 aacgcatgat tgaagcctgt gacgaaaaca ccatcggcgt ggtgcc
//
LOCUS       DAZKHF020000769          525 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2201S31241
            SAMN43267800-rid25106413.denovo.773, whole genome shotgun sequence.
ACCESSION   DAZKHF020000769 DAZKHF020000000
VERSION     DAZKHF020000769.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267800
            Sequence Read Archive: SRR30307263
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 525)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 525)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 525)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350581.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350581.2
            Genome Coverage       :: 151x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:21:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,652
            CDSs (total)                      :: 5,557
            Genes (coding)                    :: 5,320
            CDSs (with protein)               :: 5,320
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 237
            CDSs (without protein)            :: 237
            Pseudo Genes (ambiguous residues) :: 0 of 237
            Pseudo Genes (frameshifted)       :: 102 of 237
            Pseudo Genes (incomplete)         :: 130 of 237
            Pseudo Genes (internal stop)      :: 52 of 237
            Pseudo Genes (multiple problems)  :: 41 of 237
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..525
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267800-rid25106413.denovo.773"
                     /serotype="O 26:H11"
                     /isolate="2201S31241"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-01"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            <1..441
                     /locus_tag="ACHOSC_RS27380"
                     /old_locus_tag="pgaptmp_005476"
     CDS             <1..441
                     /locus_tag="ACHOSC_RS27380"
                     /old_locus_tag="pgaptmp_005476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001209482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-E factor regulatory protein RseB
                     domain-containing protein"
                     /protein_id="HGU4551177.1"
                     /translation="ALREAAQNYQSTLAWYQATPDSPNAERDCDAALAAFKRHIRHRE
                     ADIIAGLLDGLEEAKSQLNEQREYYEGVISDGSKRIAELDAREVQLPTRYDLRYGHPI
                     NADERHVMIPKENGSWLYLIDLEHALRVAGIRIKGEEHGNKTRG"
BASE COUNT          149 a          113 c          140 g          123 t
ORIGIN      
        1 gcgcttcgtg aggcagcaca aaactatcag tcgacgctgg cgtggtatca ggctaccccg
       61 gacagcccaa atgctgaacg ggattgtgat gcggctcttg ctgcgtttaa gcgtcatatc
      121 cgtcatcggg aagcggatat tatcgctggt ttgctggatg gactggaaga agcaaaatca
      181 caactcaacg agcagcgtga gtattacgaa ggcgttatct ctgatgggag caagcgtatt
      241 gctgaactgg atgcgcggga agttcaatta ccgactcgct acgaccttcg atatggacac
      301 ccgataaatg cagatgagcg acatgtcatg atacctaaag aaaatggcag ttggctttac
      361 ctgattgacc tagaacacgc attacgcgtc gctggcattc gcatcaaagg agaggagcat
      421 ggaaataaaa ccagaggatg agttaagcaa tatcgtttta tttccggtaa aagaggatga
      481 ccctcgtaat caggttaatt ttctttatga gccatcggaa agacc
//
LOCUS       DAZKHF020000770          524 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2201S31241
            SAMN43267800-rid25106413.denovo.774, whole genome shotgun sequence.
ACCESSION   DAZKHF020000770 DAZKHF020000000
VERSION     DAZKHF020000770.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267800
            Sequence Read Archive: SRR30307263
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 524)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 524)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 524)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350581.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350581.2
            Genome Coverage       :: 151x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:21:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,652
            CDSs (total)                      :: 5,557
            Genes (coding)                    :: 5,320
            CDSs (with protein)               :: 5,320
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 237
            CDSs (without protein)            :: 237
            Pseudo Genes (ambiguous residues) :: 0 of 237
            Pseudo Genes (frameshifted)       :: 102 of 237
            Pseudo Genes (incomplete)         :: 130 of 237
            Pseudo Genes (internal stop)      :: 52 of 237
            Pseudo Genes (multiple problems)  :: 41 of 237
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..524
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267800-rid25106413.denovo.774"
                     /serotype="O 26:H11"
                     /isolate="2201S31241"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-01"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            <1..>524
                     /locus_tag="ACHOSC_RS27385"
                     /old_locus_tag="pgaptmp_005477"
     CDS             <1..>524
                     /locus_tag="ACHOSC_RS27385"
                     /old_locus_tag="pgaptmp_005477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Replication protein O"
                     /protein_id="HGU4551178.1"
                     /translation="VKRCNEAKLELVRMNIIKQQGGMFGPNKNISEWCIPQNEGGSPK
                     MRDIPQNEGKSPKTRDKTSLKLGDCYPSKQGDTKDTITKEKRKDYSSENSGESSDQPE
                     NDLSVVKPDAAIQSGSKWGTAEDLTAAEWMFDMVKTIAPSARKPNFAGWANDIRLMRE
                     RDGRNHRDMCVLFR"
BASE COUNT          175 a          119 c          129 g          101 t
ORIGIN      
        1 gtcaaacggt gcaatgaagc caagttagaa ctcgtcagaa tgaatattat caagcagcaa
       61 ggcggcatgt ttggaccaaa taaaaacatc tcagaatggt gcatccctca aaacgaggga
      121 ggttccccta aaatgaggga catccctcaa aacgagggaa aatcccctaa aacgagggat
      181 aaaacatccc tcaaattagg ggattgctat ccctcaaaac agggggacac aaaagacact
      241 attacaaaag aaaaaagaaa agattattcg tccgagaatt ctggcgaatc ctctgaccag
      301 ccagaaaacg atctttctgt ggttaaaccg gatgctgcaa ttcagagcgg cagcaagtgg
      361 ggaacagcag aagacctgac cgccgcagag tggatgtttg acatggtgaa gaccatcgca
      421 ccatcagcca gaaaaccgaa ttttgcaggg tgggctaacg atatccgcct gatgcgtgaa
      481 cgtgacggac gtaaccaccg cgatatgtgt gtgcttttcc gctg
//
LOCUS       DAZKHF020000771          519 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2201S31241
            SAMN43267800-rid25106413.denovo.775, whole genome shotgun sequence.
ACCESSION   DAZKHF020000771 DAZKHF020000000
VERSION     DAZKHF020000771.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267800
            Sequence Read Archive: SRR30307263
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 519)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 519)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 519)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350581.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350581.2
            Genome Coverage       :: 151x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:21:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,652
            CDSs (total)                      :: 5,557
            Genes (coding)                    :: 5,320
            CDSs (with protein)               :: 5,320
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 237
            CDSs (without protein)            :: 237
            Pseudo Genes (ambiguous residues) :: 0 of 237
            Pseudo Genes (frameshifted)       :: 102 of 237
            Pseudo Genes (incomplete)         :: 130 of 237
            Pseudo Genes (internal stop)      :: 52 of 237
            Pseudo Genes (multiple problems)  :: 41 of 237
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..519
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267800-rid25106413.denovo.775"
                     /serotype="O 26:H11"
                     /isolate="2201S31241"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-01"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            complement(<1..71)
                     /locus_tag="ACHOSC_RS27390"
                     /old_locus_tag="pgaptmp_005478"
     CDS             complement(<1..71)
                     /locus_tag="ACHOSC_RS27390"
                     /old_locus_tag="pgaptmp_005478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4551179.1"
                     /translation="MIETLLDFSGLEDISRDLQLLSGA"
     gene            complement(68..418)
                     /locus_tag="ACHOSC_RS27395"
                     /old_locus_tag="pgaptmp_005479"
     CDS             complement(68..418)
                     /locus_tag="ACHOSC_RS27395"
                     /old_locus_tag="pgaptmp_005479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage head closure protein"
                     /protein_id="HGU4551180.1"
                     /translation="MAISAGRLTQMISVLNPVLTRNAAGEMTEEWVSCGKIHADIRGR
                     SSRERMQSGAEMAQAEIRIWVRGQSGREITAASRLHVLSGPWRDRILNVVGLPVPDAT
                     GGRLEILCRLGGEK"
     gene            complement(429..>519)
                     /locus_tag="ACHOSC_RS27400"
                     /old_locus_tag="pgaptmp_005480"
     CDS             complement(429..>519)
                     /locus_tag="ACHOSC_RS27400"
                     /old_locus_tag="pgaptmp_005480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4551181.1"
                     /translation="LAEREDLVQGELPFSVSVLIYDLRCPTVL"
BASE COUNT          115 a          185 c          116 g          103 t
ORIGIN      
        1 gcaccactca gaagctgcaa atcgcggctg atgtcctcca gccccgaaaa atccagcagg
       61 gtttcgatca tttttcccct cccagccgac agagaatttc cagacgcccg ccggtcgcat
      121 ccggcacggg cagcccgaca acgttcagga tccggtcacg ccatggacca ctcagcacat
      181 gaagtcgtga cgctgccgtg atttcccggc cggactgacc gcgcacccag atgcggattt
      241 ccgcctgcgc catttccgca ccggactgca tccgctcccg gctgctcctg ccacggatat
      301 ccgcatgaat tttcccgcat gacacccatt cttccgtcat ttctccggca gcattacggg
      361 ttaacaccgg gttcagaaca cttatcatct gtgtcagacg acctgcagat attgccattc
      421 ctccctcctc ataacaccgt cggacaacgc aaatcgtaaa tcagcacgga cacagaaaac
      481 ggcagttccc cctgcacgag gtcttcccgc tcagcaaga
//
LOCUS       DAZKHF020000772          515 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2201S31241
            SAMN43267800-rid25106413.denovo.776, whole genome shotgun sequence.
ACCESSION   DAZKHF020000772 DAZKHF020000000
VERSION     DAZKHF020000772.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267800
            Sequence Read Archive: SRR30307263
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 515)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 515)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 515)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350581.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350581.2
            Genome Coverage       :: 151x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:21:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,652
            CDSs (total)                      :: 5,557
            Genes (coding)                    :: 5,320
            CDSs (with protein)               :: 5,320
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 237
            CDSs (without protein)            :: 237
            Pseudo Genes (ambiguous residues) :: 0 of 237
            Pseudo Genes (frameshifted)       :: 102 of 237
            Pseudo Genes (incomplete)         :: 130 of 237
            Pseudo Genes (internal stop)      :: 52 of 237
            Pseudo Genes (multiple problems)  :: 41 of 237
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..515
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267800-rid25106413.denovo.776"
                     /serotype="O 26:H11"
                     /isolate="2201S31241"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-01"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
BASE COUNT          164 a           97 c          102 g          152 t
ORIGIN      
        1 aactaaaaaa acaatggata aaaccaataa cattgcataa taatacacct cccttataaa
       61 taatggagaa gaaaatgaaa aggcggtata tcacctatgc tgaacctgtt taggatgctg
      121 tgtaaatgcc ttttctccga agtgaccgtc caagcggtca ccgaattcga taataaagcg
      181 gctcattgcc atacaccagt ccttcaacgg catcgtccat ttctgaggcg cagactggat
      241 agctaaagtg tagcggtatt aggagctgtt atttagttcg aatatcgaaa atttttgtgt
      301 acgttggtcg tatcaatctc tttttatttg aattagtttg ataacagatt tttgcaatct
      361 gataactcat ttatttattg agggataaat gcaatattgt acaatatgtt tccgccatta
      421 gcaaaagaat ccacaaggcg cttgcactaa tgcatggtaa tgcctccaat tagttgaatt
      481 aggggtatat gaaccgcccc gggaatcctg gagac
//
LOCUS       DAZKHF020000773          514 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2201S31241
            SAMN43267800-rid25106413.denovo.777, whole genome shotgun sequence.
ACCESSION   DAZKHF020000773 DAZKHF020000000
VERSION     DAZKHF020000773.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267800
            Sequence Read Archive: SRR30307263
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 514)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 514)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 514)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350581.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350581.2
            Genome Coverage       :: 151x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:21:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,652
            CDSs (total)                      :: 5,557
            Genes (coding)                    :: 5,320
            CDSs (with protein)               :: 5,320
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 237
            CDSs (without protein)            :: 237
            Pseudo Genes (ambiguous residues) :: 0 of 237
            Pseudo Genes (frameshifted)       :: 102 of 237
            Pseudo Genes (incomplete)         :: 130 of 237
            Pseudo Genes (internal stop)      :: 52 of 237
            Pseudo Genes (multiple problems)  :: 41 of 237
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..514
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267800-rid25106413.denovo.777"
                     /serotype="O 26:H11"
                     /isolate="2201S31241"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-01"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            complement(<1..489)
                     /locus_tag="ACHOSC_RS27405"
                     /old_locus_tag="pgaptmp_005481"
     CDS             complement(<1..489)
                     /locus_tag="ACHOSC_RS27405"
                     /old_locus_tag="pgaptmp_005481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4551182.1"
                     /translation="MILFADYNTPYLFAISFVLLIGLLEILALICGHMLSGALDAHLD
                     HYDSITTGHISQALHYLNIGRLPALVVLCLLAGFFGLIGILLQHACIMVWQSPLSNLF
                     VVPVSLLFTIIAVHYTGKIVAPWIPRDHSSAITEEEYIGSMALITGHQATSGNPCEGK
                     LTD"
BASE COUNT          170 a          103 c          123 g          118 t
ORIGIN      
        1 atcggtgagt tttccttcgc agggattacc tgaagtggct tgatgaccgg taattaatgc
       61 catgctacca atatattctt cttctgtaat agctgaacta tggtcgcgtg gaatccaggg
      121 cgcaactatt ttaccggtat aatgcaccgc aataatcgta aacagtaaac tgacaggaac
      181 cacgaacagg ttcgacagtg gtgattgcca gaccatgatg caggcatgct gcaacaggat
      241 gccaataaga ccaaaaaaac cagccagcaa gcagagaacg acgagagctg gcaatcttcc
      301 gatattgaga taatgaagtg cctggctaat atgacctgtg gttatggaat cataatggtc
      361 aagatgtgca tcaagagcac cggagagcat gtgaccgcag ataagcgcta aaatttctaa
      421 aaggccgatc aacagtacaa aggaaatagc gaaaagataa ggggtattat agtcggcgaa
      481 taaaatcata tgtcactcca atgtccattt agtg
//
LOCUS       DAZKHF020000774          509 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2201S31241
            SAMN43267800-rid25106413.denovo.778, whole genome shotgun sequence.
ACCESSION   DAZKHF020000774 DAZKHF020000000
VERSION     DAZKHF020000774.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267800
            Sequence Read Archive: SRR30307263
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 509)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 509)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 509)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350581.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350581.2
            Genome Coverage       :: 151x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:21:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,652
            CDSs (total)                      :: 5,557
            Genes (coding)                    :: 5,320
            CDSs (with protein)               :: 5,320
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 237
            CDSs (without protein)            :: 237
            Pseudo Genes (ambiguous residues) :: 0 of 237
            Pseudo Genes (frameshifted)       :: 102 of 237
            Pseudo Genes (incomplete)         :: 130 of 237
            Pseudo Genes (internal stop)      :: 52 of 237
            Pseudo Genes (multiple problems)  :: 41 of 237
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..509
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267800-rid25106413.denovo.778"
                     /serotype="O 26:H11"
                     /isolate="2201S31241"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-01"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            complement(<1..>509)
                     /locus_tag="ACHOSC_RS27410"
                     /old_locus_tag="pgaptmp_005482"
     CDS             complement(<1..>509)
                     /locus_tag="ACHOSC_RS27410"
                     /old_locus_tag="pgaptmp_005482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030430 - host cell cytoplasm [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0046718 - symbiont entry into host cell
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage minor tail protein L"
                     /protein_id="HGU4551183.1"
                     /translation="TWQGRKYEAYPIDGSGFEMNGRGSSARPSLTVSNLFGLVTGMAE
                     DLQSLVGATVVRRRVYARFLDAVNFVAGNPEADPEQELSDRWVVEQMSQLTAMTASFV
                     LATPTETDGALFPGRIMLANTCMWDYRGDECGYNGPAVADEFDNPTTDIRKDRCSKCM
                     RGCELRRNVG"
BASE COUNT           99 a          190 c          128 g           92 t
ORIGIN      
        1 ccgacattcc tgcgcagttc acacccgcgc atgcacttgc tgcatctgtc cttacggata
       61 tccgtggtgg ggttgtcgaa ctcatccgcc accgcaggac cgttataccc gcattcatct
      121 ccccggtaat cccacataca ggtgttcgcc agcatgatgc gaccgggaaa cagcgcccca
      181 tccgtctcgg tcggtgtagc cagcacaaac gaggccgtca tggctgtcag ctgcgacatc
      241 tgctccacca cccagcggtc actcagctcc tgctccgggt ccgcctccgg attgcccgca
      301 acgaaattca ccgcatccag aaaacgggca tacacccggc ggcggaccac cgtggccccc
      361 accagactct gcaggtcctc cgccatcccg gtgacaaggc caaacagatt ggacaccgtc
      421 agcgacgggc gggcactgct gccccggccg ttcatctcaa agccgctgcc gtcaatcggg
      481 tatgcctcat acttacgccc ctgccaggt
//
LOCUS       DAZKHF020000775          508 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2201S31241
            SAMN43267800-rid25106413.denovo.779, whole genome shotgun sequence.
ACCESSION   DAZKHF020000775 DAZKHF020000000
VERSION     DAZKHF020000775.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267800
            Sequence Read Archive: SRR30307263
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 508)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 508)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 508)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350581.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350581.2
            Genome Coverage       :: 151x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:21:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,652
            CDSs (total)                      :: 5,557
            Genes (coding)                    :: 5,320
            CDSs (with protein)               :: 5,320
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 237
            CDSs (without protein)            :: 237
            Pseudo Genes (ambiguous residues) :: 0 of 237
            Pseudo Genes (frameshifted)       :: 102 of 237
            Pseudo Genes (incomplete)         :: 130 of 237
            Pseudo Genes (internal stop)      :: 52 of 237
            Pseudo Genes (multiple problems)  :: 41 of 237
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..508
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267800-rid25106413.denovo.779"
                     /serotype="O 26:H11"
                     /isolate="2201S31241"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-01"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            complement(<1..458)
                     /locus_tag="ACHOSC_RS27415"
                     /old_locus_tag="pgaptmp_005483"
     CDS             complement(<1..458)
                     /locus_tag="ACHOSC_RS27415"
                     /old_locus_tag="pgaptmp_005483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308843.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4551184.1"
                     /translation="MLSSCIGVVMKDGALLRSSSLFIAYMGCLGWGSAYFYGWGTSFY
                     YGFPWWIVGAGVDDVARSLFFAVIVIAIFLIGWGIGVVFFFAVKRKHSMQELNVFRLY
                     FAVELLFVPAIIEFSILRQKIQVPLLLLSAAIALAVTISIRSYGRFLSVS"
BASE COUNT          199 a          129 c           76 g          104 t
ORIGIN      
        1 catgataccg ataaaaatcg cccataagat cttatcgaaa ttgtaaccgc cagcgcaatc
       61 gctgctgaca gtagcaaaag aggtacctga atcttctgtc tcaatataga aaactcaata
      121 attgccggca caaacaataa ttccacagca aaataaaggc gaaatacatt tagctcttgc
      181 atagaatgtt ttcttttcac tgcgaaaaag aatacaacac caatacccca accgataaga
      241 aatatagcaa tgacgataac tgcaaaaaat aaacttctgg caacatcatc aacacctgca
      301 cctacaatcc accatgggaa gccgtagtaa aaagaagtac cccatccata gaaataagca
      361 ctcccccatc caaggcatcc catgtaggca ataaaaagtg aagaactcct gagcagcgca
      421 ccatccttca taaccacccc aatacaagat gataacattg gcttacaact cataacaaaa
      481 gcaattcaat gccgtcaaga ggttacag
//
LOCUS       DAZKHF020000776          508 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2201S31241
            SAMN43267800-rid25106413.denovo.780, whole genome shotgun sequence.
ACCESSION   DAZKHF020000776 DAZKHF020000000
VERSION     DAZKHF020000776.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267800
            Sequence Read Archive: SRR30307263
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 508)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 508)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 508)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350581.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350581.2
            Genome Coverage       :: 151x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:21:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,652
            CDSs (total)                      :: 5,557
            Genes (coding)                    :: 5,320
            CDSs (with protein)               :: 5,320
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 237
            CDSs (without protein)            :: 237
            Pseudo Genes (ambiguous residues) :: 0 of 237
            Pseudo Genes (frameshifted)       :: 102 of 237
            Pseudo Genes (incomplete)         :: 130 of 237
            Pseudo Genes (internal stop)      :: 52 of 237
            Pseudo Genes (multiple problems)  :: 41 of 237
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..508
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267800-rid25106413.denovo.780"
                     /serotype="O 26:H11"
                     /isolate="2201S31241"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-01"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            <1..>508
                     /locus_tag="ACHOSC_RS27420"
                     /old_locus_tag="pgaptmp_005484"
     CDS             <1..>508
                     /locus_tag="ACHOSC_RS27420"
                     /old_locus_tag="pgaptmp_005484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HGU4551185.1"
                     /translation="RHAEEAKKNAGEAETSARNAGISASKAEASAANADTSAEDASES
                     ARQAAESAASAKKSEEASSSSASEAAQKASESLQSATDAELSKKTAESAAGNASRDAT
                     TATEKARESAESAQSAEQSRIAAEEAVNRIPTVVGPPGPKGDPGPAGPQGPKGDKGER
                     GDTGPAGAT"
BASE COUNT          152 a          133 c          171 g           52 t
ORIGIN      
        1 gcgtcacgca gaggaggcga agaagaatgc cggagaagca gagacgtccg cgaggaatgc
       61 cggcatatca gccagtaagg cggaagcgag cgccgcaaat gctgatactt cagcagagga
      121 tgcatcggag tcagcccggc aggcggcaga aagtgcagcc tctgcaaaga agtcagagga
      181 agcgtcctcg tcctcagcct ctgaggccgc tcaaaaagcc agtgagtcat tacaaagtgc
      241 aacagatgcc gagttgtcaa aaaagacggc agaaagtgca gccggtaatg catccagaga
      301 cgcaacgaca gcaacagaaa aagcccggga gtcagcagaa agcgcacagt cagcggaaca
      361 aagcaggata gcggcggaag aagccgtaaa ccgaatcccc accgtggtgg ggccacccgg
      421 gccaaaggga gatccggggc ccgcgggtcc tcaggggccg aagggagata aaggagagcg
      481 tggcgacacc ggcccggcag gggcaact
//
LOCUS       DAZKHF020000777          505 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2201S31241
            SAMN43267800-rid25106413.denovo.781, whole genome shotgun sequence.
ACCESSION   DAZKHF020000777 DAZKHF020000000
VERSION     DAZKHF020000777.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267800
            Sequence Read Archive: SRR30307263
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 505)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 505)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 505)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350581.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350581.2
            Genome Coverage       :: 151x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:21:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,652
            CDSs (total)                      :: 5,557
            Genes (coding)                    :: 5,320
            CDSs (with protein)               :: 5,320
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 237
            CDSs (without protein)            :: 237
            Pseudo Genes (ambiguous residues) :: 0 of 237
            Pseudo Genes (frameshifted)       :: 102 of 237
            Pseudo Genes (incomplete)         :: 130 of 237
            Pseudo Genes (internal stop)      :: 52 of 237
            Pseudo Genes (multiple problems)  :: 41 of 237
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..505
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267800-rid25106413.denovo.781"
                     /serotype="O 26:H11"
                     /isolate="2201S31241"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-01"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            complement(22..>505)
                     /locus_tag="ACHOSC_RS27425"
                     /old_locus_tag="pgaptmp_005485"
     CDS             complement(22..>505)
                     /locus_tag="ACHOSC_RS27425"
                     /old_locus_tag="pgaptmp_005485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000908053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4551186.1"
                     /translation="KLGKYIFSSRAPQVLELAIGWRGALSESIKGVKFCIWFSVAWRT
                     IEFIMSSERDLVNFLGDFSMDVAKAVIAGGVATAIGSLASFACVSFGFPVILVGGAIL
                     LTGIVCTVVLNEIDAQCHLSEKLKYAIRDGLKRQQELDKWKRENMTPFMYVLNTPPVI
                     "
BASE COUNT          160 a          115 c           85 g          145 t
ORIGIN      
        1 atgtaaacgc atggttaatc ctcatatcac gggtggagtg ttaagaacat acataaatgg
       61 agtcatgttt tcccttttcc atttatcaag ttcctgttgc cgttttagtc catctctaat
      121 tgcatatttt aatttttctg ataaatggca ttgagcatcg atttcattta aaacaactgt
      181 acacactatc cctgtcagta aaattgctcc tccgacaaga attactggaa agccaaagct
      241 aacacaggca aaagaagcca ggcttccaat agcggttgca acaccaccgg ctataactgc
      301 tttggccaca tccattgaaa aatcgccaag aaaattaacc agatctcgct ctgaactcat
      361 aataaattct atggttctcc aggctactga aaaccagata cagaacttaa ctccttttat
      421 tgattcagat aaagctcctc gccagccaat agcgagttcc agaacttgag gcgctcgtga
      481 agagaaaata tatttaccga gcttt
//
LOCUS       DAZKHF020000778          504 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2201S31241
            SAMN43267800-rid25106413.denovo.782, whole genome shotgun sequence.
ACCESSION   DAZKHF020000778 DAZKHF020000000
VERSION     DAZKHF020000778.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267800
            Sequence Read Archive: SRR30307263
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 504)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 504)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 504)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350581.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350581.2
            Genome Coverage       :: 151x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:21:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,652
            CDSs (total)                      :: 5,557
            Genes (coding)                    :: 5,320
            CDSs (with protein)               :: 5,320
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 237
            CDSs (without protein)            :: 237
            Pseudo Genes (ambiguous residues) :: 0 of 237
            Pseudo Genes (frameshifted)       :: 102 of 237
            Pseudo Genes (incomplete)         :: 130 of 237
            Pseudo Genes (internal stop)      :: 52 of 237
            Pseudo Genes (multiple problems)  :: 41 of 237
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..504
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267800-rid25106413.denovo.782"
                     /serotype="O 26:H11"
                     /isolate="2201S31241"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-01"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            <1..456
                     /locus_tag="ACHOSC_RS27430"
                     /old_locus_tag="pgaptmp_005486"
     CDS             <1..456
                     /locus_tag="ACHOSC_RS27430"
                     /old_locus_tag="pgaptmp_005486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001427568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4551187.1"
                     /translation="IVKELESDYSSLMKRLYGPLLKLHKPIEEQNYTVELKRRRTNLA
                     RYDHTTLENLIVERADDERIIIEASITRFNRLTGTGRLILEEDSESVSFEPSYLWRDF
                     PADQKRWLSRNLDVNTDNGDFTRVRLEVTSIRNFLGVIKKYSLHRVITD"
BASE COUNT          162 a           80 c          107 g          155 t
ORIGIN      
        1 attgttaaag aacttgagtc agattatagt tcattaatga agaggcttta tggcccacta
       61 ttgaagcttc ataagcccat agaagaacaa aattatactg ttgagctgaa acggagaagg
      121 acaaatcttg ctcggtatga tcacacgacc cttgaaaatc taattgttga acgtgccgat
      181 gatgaaagga tcattattga agcttctatt acaagattta atcggctaac ggggacggga
      241 aggctcattc ttgaggaaga ttctgagtct gtaagttttg aaccttcata tctttggaga
      301 gattttccag cagatcaaaa acgctggcta tctagaaact tagacgtcaa cacagataat
      361 ggtgatttta ctcgtgtacg gctagaggtg acatcaatca gaaactttct tggtgtcatt
      421 aaaaaatata gtcttcaccg agttataacg gattgatatg ataaagtatg ttgccttttt
      481 agttatatca gttacatggt tgat
//
LOCUS       DAZKHF020000779          504 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2201S31241
            SAMN43267800-rid25106413.denovo.783, whole genome shotgun sequence.
ACCESSION   DAZKHF020000779 DAZKHF020000000
VERSION     DAZKHF020000779.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267800
            Sequence Read Archive: SRR30307263
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 504)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 504)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 504)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350581.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350581.2
            Genome Coverage       :: 151x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:21:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,652
            CDSs (total)                      :: 5,557
            Genes (coding)                    :: 5,320
            CDSs (with protein)               :: 5,320
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 237
            CDSs (without protein)            :: 237
            Pseudo Genes (ambiguous residues) :: 0 of 237
            Pseudo Genes (frameshifted)       :: 102 of 237
            Pseudo Genes (incomplete)         :: 130 of 237
            Pseudo Genes (internal stop)      :: 52 of 237
            Pseudo Genes (multiple problems)  :: 41 of 237
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..504
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267800-rid25106413.denovo.783"
                     /serotype="O 26:H11"
                     /isolate="2201S31241"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-01"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            <1..>504
                     /locus_tag="ACHOSC_RS27435"
                     /old_locus_tag="pgaptmp_005487"
     CDS             <1..>504
                     /locus_tag="ACHOSC_RS27435"
                     /old_locus_tag="pgaptmp_005487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HGU4551188.1"
                     /translation="QMVKEAVSHIPVPRDGRDYDPEVLQKAVNDAVANIPVPADGKSI
                     TPDDVRPMLEQMVKEAVSHIPVPRDGRDYDPDVLQKAVLEAVNALPAPQDGRDATALE
                     VLPAIDDQKSFPRGTYATHLGGFWRAYEKTHGMRGWECLVDGVADIDVSMTGERLFSV
                     VIRHSSGQ"
BASE COUNT          107 a          116 c          172 g          109 t
ORIGIN      
        1 cagatggtga aagaggcggt aagccatatt cctgttccgc gtgatggtcg tgattacgat
       61 ccggaagtac tgcagaaggc ggtgaatgat gcggtcgcga atattccggt accggcggac
      121 ggcaaaagta ttacccccga tgatgtgcgt ccgatgcttg aacagatggt gaaagaggcg
      181 gtaagccata ttcctgttcc gcgtgatggt cgtgactatg atcccgatgt tctgcagaag
      241 gctgttctgg aggcggtgaa tgccctgccg gctccgcagg acgggcgtga tgccacggct
      301 ctggaagtgc tccccgctat tgacgatcaa aaatcctttc cccggggcac gtatgccaca
      361 cacctgggag gattctggcg ggcgtatgaa aaaacgcacg gaatgcgggg atgggaatgc
      421 ctggttgacg gggtggcgga tattgacgtc agtatgacgg gggagcggtt gttctctgtg
      481 gtgatccggc acagcagtgg ccag
//
LOCUS       DAZKHF020000780          504 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2201S31241
            SAMN43267800-rid25106413.denovo.784, whole genome shotgun sequence.
ACCESSION   DAZKHF020000780 DAZKHF020000000
VERSION     DAZKHF020000780.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267800
            Sequence Read Archive: SRR30307263
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 504)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 504)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 504)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350581.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350581.2
            Genome Coverage       :: 151x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:21:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,652
            CDSs (total)                      :: 5,557
            Genes (coding)                    :: 5,320
            CDSs (with protein)               :: 5,320
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 237
            CDSs (without protein)            :: 237
            Pseudo Genes (ambiguous residues) :: 0 of 237
            Pseudo Genes (frameshifted)       :: 102 of 237
            Pseudo Genes (incomplete)         :: 130 of 237
            Pseudo Genes (internal stop)      :: 52 of 237
            Pseudo Genes (multiple problems)  :: 41 of 237
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..504
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267800-rid25106413.denovo.784"
                     /serotype="O 26:H11"
                     /isolate="2201S31241"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-01"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            <1..>504
                     /locus_tag="ACHOSC_RS27440"
                     /old_locus_tag="pgaptmp_005488"
     CDS             <1..>504
                     /locus_tag="ACHOSC_RS27440"
                     /old_locus_tag="pgaptmp_005488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="phage tail length tape measure family protein"
                     /protein_id="HGU4551189.1"
                     /translation="YAWYQGDSTLSAFNKTLVLSGNQSGLTADRMLTLSRAGQAAGLT
                     FNQASESLAALVNAGVRGGEQFDAINQSVARFASASGVEVDKVAEAFGKLTTDPTSGL
                     IAMARQFRNVTAEQIAYVAQLQRSGDEAGALQAANDIATKGFDEQTRRLKENMGTLET
                     WADRTARA"
BASE COUNT          103 a          132 c          167 g          102 t
ORIGIN      
        1 gtacgcctgg taccaggggg attccacgct ttcagcgttt aataaaaccc tggttctttc
       61 cggtaatcag tccggactga ctgccgatcg catgttgacg ctctccagag ccggacaggc
      121 cgcagggctg acgtttaacc aggcgagtga gtcactggca gccctggtga atgccggtgt
      181 gcgtggtggt gaacagtttg atgccatcaa ccagagtgtc gcgcgttttg cttctgcatc
      241 cggtgtggag gtggacaagg ttgcagaggc tttcggaaaa ctgaccaccg accctacgtc
      301 ggggctgatt gcgatggcgc gccagttccg taacgtgacg gcagagcaga ttgcgtatgt
      361 tgcgcagctg cagcgttccg gtgatgaggc cggggcctta caggcggcga acgatatcgc
      421 cacgaaaggc tttgatgagc agacccgccg cctgaaagag aacatgggca cgctggagac
      481 ctgggcagac aggactgcac gggc
//
LOCUS       DAZKHF020000781          503 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2201S31241
            SAMN43267800-rid25106413.denovo.785, whole genome shotgun sequence.
ACCESSION   DAZKHF020000781 DAZKHF020000000
VERSION     DAZKHF020000781.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267800
            Sequence Read Archive: SRR30307263
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 503)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 503)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 503)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350581.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350581.2
            Genome Coverage       :: 151x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:21:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,652
            CDSs (total)                      :: 5,557
            Genes (coding)                    :: 5,320
            CDSs (with protein)               :: 5,320
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 237
            CDSs (without protein)            :: 237
            Pseudo Genes (ambiguous residues) :: 0 of 237
            Pseudo Genes (frameshifted)       :: 102 of 237
            Pseudo Genes (incomplete)         :: 130 of 237
            Pseudo Genes (internal stop)      :: 52 of 237
            Pseudo Genes (multiple problems)  :: 41 of 237
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..503
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267800-rid25106413.denovo.785"
                     /serotype="O 26:H11"
                     /isolate="2201S31241"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-01"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            complement(<1..>503)
                     /locus_tag="ACHOSC_RS27445"
                     /old_locus_tag="pgaptmp_005489"
     CDS             complement(<1..>503)
                     /locus_tag="ACHOSC_RS27445"
                     /old_locus_tag="pgaptmp_005489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000609979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HGU4551190.1"
                     /translation="FKSSASTIKRKINDLKEMGLLETSPHPDPYISSLIYNALPLTDA
                     HITPPGESSLSDLSEAEEAQEQPKGHKQPSFDVLDDDWDTPLPWETEETPVSSSEKVA
                     NDNEEDVLKNFASLIIKQKNTRTGGASFIEFATSLAYKHRLKLPNGIEAYFAKKHPKV
                     YEDFDIP"
BASE COUNT          142 a           94 c           99 g          168 t
ORIGIN      
        1 tggaatatca aagtcttcgt atactttggg atgtttctta gcaaaatatg cctcaatacc
       61 atttggtagc tttaacctgt gcttataggc taacgatgtt gcaaattcta taaatgatgc
      121 tcctcctgtt ctagtattct tctgcttgat aataagagag gcaaaattct ttaacacatc
      181 ctcctcatta tcattagcta ccttttcact tgatgaaaca ggtgtttctt ccgtctccca
      241 cggtaaaggc gtatcccaat catcatcaag aacatcaaaa gaaggctgtt tatggccttt
      301 aggttgttcc tgtgcttctt ctgcctcaga aagatcagag agtgatgatt ctcctggggg
      361 agtaatatgt gcatctgtga gagggagagc attgtaaatc aaagatgaaa tatacggatc
      421 aggatgtggg gatgtttcta ataatcccat ctctttaaga tcgtttatct tacgtttaat
      481 tgttgacgct gaagacttaa aaa
//
LOCUS       DAZKHF020000782          502 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2201S31241
            SAMN43267800-rid25106413.denovo.786, whole genome shotgun sequence.
ACCESSION   DAZKHF020000782 DAZKHF020000000
VERSION     DAZKHF020000782.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267800
            Sequence Read Archive: SRR30307263
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 502)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 502)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 502)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350581.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350581.2
            Genome Coverage       :: 151x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:21:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,652
            CDSs (total)                      :: 5,557
            Genes (coding)                    :: 5,320
            CDSs (with protein)               :: 5,320
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 237
            CDSs (without protein)            :: 237
            Pseudo Genes (ambiguous residues) :: 0 of 237
            Pseudo Genes (frameshifted)       :: 102 of 237
            Pseudo Genes (incomplete)         :: 130 of 237
            Pseudo Genes (internal stop)      :: 52 of 237
            Pseudo Genes (multiple problems)  :: 41 of 237
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..502
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267800-rid25106413.denovo.786"
                     /serotype="O 26:H11"
                     /isolate="2201S31241"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-01"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
BASE COUNT          139 a          105 c          126 g          132 t
ORIGIN      
        1 aaagtagatc gcagaaaata aaaaaacctg ctggtgaggc aggttctttt atttactaca
       61 gtgacgctaa cttgctgatt gtgcaacatt tcgtaccaac gaataaatta taaccatatc
      121 cacactccat tgcaattcag tgagtgagat ttataatcct gcccgttggg aggttgtgcc
      181 ggatcggacc aattttaagt cctggctgcc ggacgggtgg tgccgcaggc ggtgccctga
      241 tccggagagc aacgtttcgt accaacatac gtaaaccagt atcaacaggc agtgtaaggg
      301 taaggtgctg gtgttgcgct ccttaataag gagagcggat ggacacgctg acacaaaagt
      361 taaccgtgct cattgccgta ctggagttat tagtggctct gttacggttg attgatttgt
      421 tgaagtaacg ggcaaattga aacgcatcta agggggaata ccgtttcccc ctttaagaaa
      481 gctatactcg tcatacttca ag
//
LOCUS       DAZKHF020000783          502 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2201S31241
            SAMN43267800-rid25106413.denovo.787, whole genome shotgun sequence.
ACCESSION   DAZKHF020000783 DAZKHF020000000
VERSION     DAZKHF020000783.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267800
            Sequence Read Archive: SRR30307263
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 502)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 502)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 502)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350581.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350581.2
            Genome Coverage       :: 151x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:21:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,652
            CDSs (total)                      :: 5,557
            Genes (coding)                    :: 5,320
            CDSs (with protein)               :: 5,320
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 237
            CDSs (without protein)            :: 237
            Pseudo Genes (ambiguous residues) :: 0 of 237
            Pseudo Genes (frameshifted)       :: 102 of 237
            Pseudo Genes (incomplete)         :: 130 of 237
            Pseudo Genes (internal stop)      :: 52 of 237
            Pseudo Genes (multiple problems)  :: 41 of 237
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..502
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267800-rid25106413.denovo.787"
                     /serotype="O 26:H11"
                     /isolate="2201S31241"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-01"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            complement(<1..387)
                     /locus_tag="ACHOSC_RS27450"
                     /old_locus_tag="pgaptmp_005490"
     CDS             complement(<1..387)
                     /locus_tag="ACHOSC_RS27450"
                     /old_locus_tag="pgaptmp_005490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage minor tail U family protein"
                     /protein_id="HGU4551191.1"
                     /translation="MKHTELRAAVLDALEKHDTGATLFDGRPAVFDEADFPAVAVYLT
                     GAEYTGEELDSDTWQAELHIEVFLPAQVPDSELDAWMESRIYPVMSDIPALAGLITTM
                     VTQGYEYRRDDDMALWSSADLTYSITY"
     gene            complement(384..>502)
                     /locus_tag="ACHOSC_RS27455"
                     /old_locus_tag="pgaptmp_005491"
     CDS             complement(384..>502)
                     /locus_tag="ACHOSC_RS27455"
                     /old_locus_tag="pgaptmp_005491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HGU4551192.1"
                     /translation="AVPLTTAFKQNIERIRRERLPKELGYALQHQLRMVIKR"
BASE COUNT          122 a          151 c          115 g          114 t
ORIGIN      
        1 gtatgtaatg gaataagtca gatctgcaga actccataac gccatatcgt catcacgacg
       61 atactcatag ccctgcgtaa ccatcgtggt aatcagtcct gccagtgccg ggatatcgct
      121 catcaccgga taaatccggg actccatcca cgcatccagc tctgaatccg gcacctgagc
      181 aggcaggaaa acttcaatat gcagctccgc ctgccaggta tcgctgtcca gctcttcgcc
      241 cgtgtattca gcgccggtga gataaacggc aactgccgga aaatccgcct catcaaaaac
      301 agcggggcga ccatcaaaaa gcgtcgcccc ggtgtcatgc ttctccagtg catccagtac
      361 ggctgcacgg agttcagtat gtttcatcgc tttattacca ttctcagttg atgctgcagc
      421 gcatagccca gctctttcgg aagacgttca cgccgtatcc gttcaatatt ctgtttaaac
      481 gccgtggtaa gcggcaccgc ca
//
LOCUS       DAZKHF020000784          501 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2201S31241
            SAMN43267800-rid25106413.denovo.788, whole genome shotgun sequence.
ACCESSION   DAZKHF020000784 DAZKHF020000000
VERSION     DAZKHF020000784.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267800
            Sequence Read Archive: SRR30307263
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 501)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 501)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 501)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350581.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350581.2
            Genome Coverage       :: 151x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:21:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,652
            CDSs (total)                      :: 5,557
            Genes (coding)                    :: 5,320
            CDSs (with protein)               :: 5,320
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 237
            CDSs (without protein)            :: 237
            Pseudo Genes (ambiguous residues) :: 0 of 237
            Pseudo Genes (frameshifted)       :: 102 of 237
            Pseudo Genes (incomplete)         :: 130 of 237
            Pseudo Genes (internal stop)      :: 52 of 237
            Pseudo Genes (multiple problems)  :: 41 of 237
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..501
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267800-rid25106413.denovo.788"
                     /serotype="O 26:H11"
                     /isolate="2201S31241"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-01"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            1..501
                     /locus_tag="ACHOSC_RS27460"
                     /old_locus_tag="pgaptmp_005492"
                     /pseudo
     CDS             1..501
                     /locus_tag="ACHOSC_RS27460"
                     /old_locus_tag="pgaptmp_005492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309822.1"
                     /note="frameshifted; incomplete; missing N-terminus and
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
BASE COUNT          113 a          106 c          173 g          109 t
ORIGIN      
        1 tcgtcaggca cacgccagga gggcgcgtct gacggtaata aggccataag cgaaacagag
       61 cgtgatgcgg tgaaggcgat gttcaggggg atactgcgaa aatgacagag cgtgaactgt
      121 ccattatccg tgcgctgggt gaagaattct ccacggtgct ggcggattta cagcgcacat
      181 ttgaggggaa gatggcctcg caggcacaag cgtttgaaga gaaactgact tccctgtcgg
      241 cggtattaca gaagcatgtg acggtggatg aggtgcgtcc ggttctgcag gtgatggtgg
      301 atgacgctgt gggggccatt ccggtaccgc gtgatggtcg tgattatgat ccggatgtac
      361 tgcagcaggc ggtgaatgat gcggttgcga atattccggt accggcggac ggcaaaagta
      421 ttacccccga tgatgtgcgt ccgatgcttg aacagatggt gaaggaggcg gtaagccata
      481 ttcctgttcc gcgtgatggt c
//
LOCUS       DAZKHF020000785          499 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2201S31241
            SAMN43267800-rid25106413.denovo.789, whole genome shotgun sequence.
ACCESSION   DAZKHF020000785 DAZKHF020000000
VERSION     DAZKHF020000785.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267800
            Sequence Read Archive: SRR30307263
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 499)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 499)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 499)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350581.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350581.2
            Genome Coverage       :: 151x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:21:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,652
            CDSs (total)                      :: 5,557
            Genes (coding)                    :: 5,320
            CDSs (with protein)               :: 5,320
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 237
            CDSs (without protein)            :: 237
            Pseudo Genes (ambiguous residues) :: 0 of 237
            Pseudo Genes (frameshifted)       :: 102 of 237
            Pseudo Genes (incomplete)         :: 130 of 237
            Pseudo Genes (internal stop)      :: 52 of 237
            Pseudo Genes (multiple problems)  :: 41 of 237
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..499
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267800-rid25106413.denovo.789"
                     /serotype="O 26:H11"
                     /isolate="2201S31241"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-01"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            <1..>499
                     /gene="escU"
                     /locus_tag="ACHOSC_RS27465"
                     /old_locus_tag="pgaptmp_005493"
     CDS             <1..>499
                     /gene="escU"
                     /locus_tag="ACHOSC_RS27465"
                     /old_locus_tag="pgaptmp_005493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="type III secretion system LEE export apparatus
                     switch protein EscU"
                     /protein_id="HGU4551193.1"
                     /translation="ANFTRLNAYQTLVVVAFFVFLLWKGVLFGYLLFSVFDFWFQKHE
                     GLKKMKMSKDEVKREAKDTDGNPEIKGERRRLHSEIQSGSLANNIKKSTVIVKNPTHI
                     AICLYYKLGETPLPLVIETGKDAKALQIIKLAELYDIPVIEDITLARSLYKNIHKGQY
                     ITEDFF"
BASE COUNT          167 a           88 c          100 g          144 t
ORIGIN      
        1 tgcaaatttc acaagactga acgcatatca aactcttgtc gttgttgcct tttttgtttt
       61 tcttttatgg aaaggcgtgt tattcggata tctactcttt tcagtatttg atttctggtt
      121 ccagaagcat gagggactga agaaaatgaa aatgagtaaa gatgaggtga aacgagaagc
      181 caaggatact gatggtaacc ctgaaattaa aggggagcgc cgtcgccttc attctgagat
      241 acaaagtgga agtctggcta ataacatcaa aaaatcaacc gtgatcgtca aaaacccgac
      301 tcacattgcg atttgcctat actataaact cggggagact ccattgcctt tagttattga
      361 aacaggaaaa gatgccaaag ctctacagat cattaaactg gctgaactct atgatattcc
      421 agtgattgaa gatattactt tggcaagaag tctttataag aacatacata aaggacaata
      481 cataacagaa gactttttt
//
LOCUS       DAZKHF020000786          493 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2201S31241
            SAMN43267800-rid25106413.denovo.790, whole genome shotgun sequence.
ACCESSION   DAZKHF020000786 DAZKHF020000000
VERSION     DAZKHF020000786.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267800
            Sequence Read Archive: SRR30307263
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 493)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 493)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 493)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350581.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350581.2
            Genome Coverage       :: 151x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:21:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,652
            CDSs (total)                      :: 5,557
            Genes (coding)                    :: 5,320
            CDSs (with protein)               :: 5,320
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 237
            CDSs (without protein)            :: 237
            Pseudo Genes (ambiguous residues) :: 0 of 237
            Pseudo Genes (frameshifted)       :: 102 of 237
            Pseudo Genes (incomplete)         :: 130 of 237
            Pseudo Genes (internal stop)      :: 52 of 237
            Pseudo Genes (multiple problems)  :: 41 of 237
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..493
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267800-rid25106413.denovo.790"
                     /serotype="O 26:H11"
                     /isolate="2201S31241"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-01"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            <1..471
                     /locus_tag="ACHOSC_RS27470"
                     /old_locus_tag="pgaptmp_005494"
     CDS             <1..471
                     /locus_tag="ACHOSC_RS27470"
                     /old_locus_tag="pgaptmp_005494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS3-like element IS600 family transposase"
                     /protein_id="HGU4551194.1"
                     /translation="LTYVATQEGWLYLAGIKDVYTCEIVGYAMGERMTKELTGKALFM
                     ALRSQRPPAGLIHHSDRGSQYCAYDYRVIQEQFGLKTSMSRKGNCYDNAPMESFWGTL
                     KNESLSHYRFNNRDEAISVIREYIEIFYNRQRRHSRLGNISPAAFREKYHQMAA"
BASE COUNT          139 a          114 c          127 g          113 t
ORIGIN      
        1 ctgacgtatg ttgccacaca ggagggatgg ttgtacctcg ctggcatcaa agatgtttat
       61 acgtgcgaaa ttgtcggcta cgccatggga gagcgcatga caaaagagct gacaggtaaa
      121 gctctgttta tggcgctcag gagccagcgc ccacctgccg ggctaatcca ccactctgat
      181 cgaggttcac agtactgcgc atacgattac cgggtcatac aggagcagtt tggtctgaaa
      241 acatcaatgt cgcgtaaagg taactgttac gacaacgctc cgatggaaag cttctgggga
      301 acgctgaaaa atgagagcct gagccactat cgttttaata accgggatga agccatctca
      361 gtaatacggg aatacattga gattttctac aatcgtcagc gtcgtcactc tcgtctgggg
      421 aatatctccc cggcagcctt cagggaaaaa tatcatcaga tggctgctta aaaaaagaac
      481 aaatggtagt gtc
//
LOCUS       DAZKHF020000787          489 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2201S31241
            SAMN43267800-rid25106413.denovo.791, whole genome shotgun sequence.
ACCESSION   DAZKHF020000787 DAZKHF020000000
VERSION     DAZKHF020000787.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267800
            Sequence Read Archive: SRR30307263
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 489)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 489)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 489)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350581.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350581.2
            Genome Coverage       :: 151x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:21:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,652
            CDSs (total)                      :: 5,557
            Genes (coding)                    :: 5,320
            CDSs (with protein)               :: 5,320
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 237
            CDSs (without protein)            :: 237
            Pseudo Genes (ambiguous residues) :: 0 of 237
            Pseudo Genes (frameshifted)       :: 102 of 237
            Pseudo Genes (incomplete)         :: 130 of 237
            Pseudo Genes (internal stop)      :: 52 of 237
            Pseudo Genes (multiple problems)  :: 41 of 237
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..489
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267800-rid25106413.denovo.791"
                     /serotype="O 26:H11"
                     /isolate="2201S31241"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-01"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
BASE COUNT          144 a          107 c          105 g          133 t
ORIGIN      
        1 acttcagcta ccgtcgtttc atctctccca cgaaccggag ccgcaacttg ttgcatcaca
       61 gggctgacaa gtgaactgaa atccgcacct taatattctg actggctgcg tacattcatt
      121 gcatgaagcg gccggagcgg ctgagctgaa ggtccgttgt gagcgagaag cggaagtccg
      181 aaatggtgct tattactata ggggagtgat cgtctatcgg ggtggggtca gtctaattaa
      241 cgaccctgga gacgtccaga ttatccttag cgattcaaac ctttttacga ggcccaaaga
      301 tttgatactc aaaatctcag acataaacca tctaaacata cggcttacta tcatgatgat
      361 aggggaacgg ctagcaaagg gtcaaattat cttgataact aataaaagtt ctttacatcc
      421 ataaaatcca tgatgtattt atctaatcct ttgcgattga aaaaaccgaa actttcaaag
      481 tcttttatt
//
LOCUS       DAZKHF020000788          488 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2201S31241
            SAMN43267800-rid25106413.denovo.792, whole genome shotgun sequence.
ACCESSION   DAZKHF020000788 DAZKHF020000000
VERSION     DAZKHF020000788.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267800
            Sequence Read Archive: SRR30307263
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 488)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 488)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 488)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350581.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350581.2
            Genome Coverage       :: 151x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:21:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,652
            CDSs (total)                      :: 5,557
            Genes (coding)                    :: 5,320
            CDSs (with protein)               :: 5,320
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 237
            CDSs (without protein)            :: 237
            Pseudo Genes (ambiguous residues) :: 0 of 237
            Pseudo Genes (frameshifted)       :: 102 of 237
            Pseudo Genes (incomplete)         :: 130 of 237
            Pseudo Genes (internal stop)      :: 52 of 237
            Pseudo Genes (multiple problems)  :: 41 of 237
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..488
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267800-rid25106413.denovo.792"
                     /serotype="O 26:H11"
                     /isolate="2201S31241"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-01"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            <1..>488
                     /gene="escD"
                     /locus_tag="ACHOSC_RS27475"
                     /old_locus_tag="pgaptmp_005495"
     CDS             <1..>488
                     /gene="escD"
                     /locus_tag="ACHOSC_RS27475"
                     /old_locus_tag="pgaptmp_005495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="type III secretion system LEE inner membrane
                     ring protein EscD"
                     /protein_id="HGU4551195.1"
                     /translation="KGNKPGFFLLSGYIPPSPKWSEVENLLLNTPGVAGWEIHNNSNN
                     KINELASEFKKNKLINYVNIFKKNDVIIVAGEVSQQNESKILAIINAMNKNSNVKILF
                     QNIQPYISADIFPGKILRISGTMKNPTIALDNGTSLGIGSILKGGYVIDAIDPKDGIN
                     IS"
BASE COUNT          176 a           91 c           98 g          123 t
ORIGIN      
        1 caaaaggtaa taagcctgga ttcttcctgt tatcgggata cattccccct tcaccaaaat
       61 ggagcgaagt tgaaaatctg ttactgaata cgccaggggt tgctggatgg gaaatacata
      121 acaacagtaa caataaaatc aatgaactgg catcagagtt caaaaagaat aaattaataa
      181 actatgtcaa tattttcaaa aagaatgatg tcatcattgt tgcgggggag gtttctcaac
      241 agaacgagag taagatcctt gccattataa atgcgatgaa caaaaatagt aacgtcaaga
      301 tactatttca gaacattcag ccgtacattt cagcagatat tttccccgga aaaatactca
      361 gaatcagcgg cacgatgaag aatccaacaa ttgctcttga taacggtaca agcctgggaa
      421 ttggtagcat tctgaaagga ggctatgtca tagacgcgat tgatcctaaa gatggtatca
      481 acatctcc
//
LOCUS       DAZKHF020000789          479 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2201S31241
            SAMN43267800-rid25106413.denovo.793, whole genome shotgun sequence.
ACCESSION   DAZKHF020000789 DAZKHF020000000
VERSION     DAZKHF020000789.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267800
            Sequence Read Archive: SRR30307263
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 479)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 479)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 479)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350581.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350581.2
            Genome Coverage       :: 151x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:21:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,652
            CDSs (total)                      :: 5,557
            Genes (coding)                    :: 5,320
            CDSs (with protein)               :: 5,320
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 237
            CDSs (without protein)            :: 237
            Pseudo Genes (ambiguous residues) :: 0 of 237
            Pseudo Genes (frameshifted)       :: 102 of 237
            Pseudo Genes (incomplete)         :: 130 of 237
            Pseudo Genes (internal stop)      :: 52 of 237
            Pseudo Genes (multiple problems)  :: 41 of 237
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..479
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267800-rid25106413.denovo.793"
                     /serotype="O 26:H11"
                     /isolate="2201S31241"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-01"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            complement(<1..176)
                     /locus_tag="ACHOSC_RS27480"
                     /old_locus_tag="pgaptmp_005496"
     CDS             complement(<1..176)
                     /locus_tag="ACHOSC_RS27480"
                     /old_locus_tag="pgaptmp_005496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044384 - host outer membrane [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Ail/Lom family outer membrane beta-barrel
                     protein"
                     /protein_id="HGU4551196.1"
                     /translation="MRKLCAVILSAVVWLVAAGTPASAAEHQSTLSAGYLQTHTDMPG
                     SDDLKGINVKYRYE"
     gene            complement(244..>479)
                     /locus_tag="ACHOSC_RS27485"
                     /old_locus_tag="pgaptmp_005497"
     CDS             complement(244..>479)
                     /locus_tag="ACHOSC_RS27485"
                     /old_locus_tag="pgaptmp_005497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF3672 domain-containing protein"
                     /protein_id="HGU4551197.1"
                     /translation="SSCRLIVRKNGAEIYNRTALDNTLIYSGVIDMPAGHGHMTLEFS
                     VSAWLVNDWYPTASISDLLVVVMKKATAGISIS"
BASE COUNT          123 a          127 c          107 g          122 t
ORIGIN      
        1 aattcataac ggtatttcac gttaatgccc ttcaggtcat cgctgcctgg catatcagta
       61 tgggtctgaa gatacccggc gcttagtgtg gactgatgct ctgctgcgct cgctggcgta
      121 ccagcggcaa ccagccagac tactgcggac agaataacag cacataattt acgcataatt
      181 acctctcgct tttctgcaat aaaaaaggcg ccatttctgg cgcccgtata tgggttataa
      241 aattcagctg atactgatgc ctgcggtggc tttcttcatc accacaacca gcaaatcgct
      301 gatacttgct gtgggatacc agtcatttac cagccatgct gacaccgaaa actccagcgt
      361 catgtgaccg tgaccggcag gcatatcaat aacgccactg taaatcagcg tattatccag
      421 cgcggtacgg ttataaattt cagcaccgtt tttccgcact atcagacggc atgaggagt
//
LOCUS       DAZKHF020000790          478 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2201S31241
            SAMN43267800-rid25106413.denovo.794, whole genome shotgun sequence.
ACCESSION   DAZKHF020000790 DAZKHF020000000
VERSION     DAZKHF020000790.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267800
            Sequence Read Archive: SRR30307263
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 478)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 478)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 478)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350581.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350581.2
            Genome Coverage       :: 151x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:21:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,652
            CDSs (total)                      :: 5,557
            Genes (coding)                    :: 5,320
            CDSs (with protein)               :: 5,320
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 237
            CDSs (without protein)            :: 237
            Pseudo Genes (ambiguous residues) :: 0 of 237
            Pseudo Genes (frameshifted)       :: 102 of 237
            Pseudo Genes (incomplete)         :: 130 of 237
            Pseudo Genes (internal stop)      :: 52 of 237
            Pseudo Genes (multiple problems)  :: 41 of 237
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..478
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267800-rid25106413.denovo.794"
                     /serotype="O 26:H11"
                     /isolate="2201S31241"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-01"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            <1..>478
                     /locus_tag="ACHOSC_RS27490"
                     /old_locus_tag="pgaptmp_005498"
     CDS             <1..>478
                     /locus_tag="ACHOSC_RS27490"
                     /old_locus_tag="pgaptmp_005498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="terminase large subunit"
                     /protein_id="HGU4551198.1"
                     /translation="PNLGYSVDEAFLYREYRKAREAGEEAFRGFMSKHANVEIGLALR
                     SDRWAGADFWEQQGRRVSLDDILQRADVVTVGIDGGGLDDLLGMYVIGRDRETREWLG
                     WGHAWAHETAVVRRKSEASRFQDFVACGDMTIVRRVGDDTAEVAEYVRRIHEAELL"
BASE COUNT           88 a          100 c          181 g          109 t
ORIGIN      
        1 atccgaatct cggttattcg gtggatgagg cttttctgta ccgggagtac cgtaaagccc
       61 gggaggctgg tgaggaagca tttcgtggct tcatgtcaaa acatgccaat gtggaaattg
      121 gtcttgccct gcgttctgac cgctgggcgg gtgcggattt ctgggagcag cagggcaggc
      181 gcgtcagcct ggacgatatc ctgcagcgcg ctgatgtggt gacggtgggg attgacggcg
      241 ggggcctgga tgatctgctg ggaatgtacg tgattggccg tgacagggaa acccgcgaat
      301 ggctgggctg gggccatgcc tgggcgcatg aaaccgcggt ggtcagacgg aagagtgagg
      361 catcccgttt tcaggatttt gtggcctgtg gagacatgac gattgtccgt cgggtcgggg
      421 atgacacggc ggaagtggcg gagtatgtgc gtcgtattca tgaggctgag ttactgga
//
LOCUS       DAZKHF020000791          476 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2201S31241
            SAMN43267800-rid25106413.denovo.795, whole genome shotgun sequence.
ACCESSION   DAZKHF020000791 DAZKHF020000000
VERSION     DAZKHF020000791.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267800
            Sequence Read Archive: SRR30307263
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 476)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 476)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 476)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350581.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350581.2
            Genome Coverage       :: 151x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:21:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,652
            CDSs (total)                      :: 5,557
            Genes (coding)                    :: 5,320
            CDSs (with protein)               :: 5,320
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 237
            CDSs (without protein)            :: 237
            Pseudo Genes (ambiguous residues) :: 0 of 237
            Pseudo Genes (frameshifted)       :: 102 of 237
            Pseudo Genes (incomplete)         :: 130 of 237
            Pseudo Genes (internal stop)      :: 52 of 237
            Pseudo Genes (multiple problems)  :: 41 of 237
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..476
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267800-rid25106413.denovo.795"
                     /serotype="O 26:H11"
                     /isolate="2201S31241"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-01"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            complement(<1..>476)
                     /locus_tag="ACHOSC_RS27495"
                     /old_locus_tag="pgaptmp_005499"
     CDS             complement(<1..>476)
                     /locus_tag="ACHOSC_RS27495"
                     /old_locus_tag="pgaptmp_005499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309344.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="sulfatase-like hydrolase/transferase"
                     /protein_id="HGU4551199.1"
                     /translation="IIPTNYIYSGFKYVRDFFVSPGEFRQTGTDASRTINEKQKPVIM
                     FLVVGETARSQNYALNGYSRGTNDFTKKYNELISFHNVQSCGTSTAISVPCMFSDMKR
                     KEFNSRKAVNSENVLDILYRTGVNLLWIENDGGCKGVCKRIPTINIEPSNSDNTLC"
BASE COUNT          141 a           99 c           78 g          158 t
ORIGIN      
        1 gcataatgta ttatctgaat ttgatggctc aatattaatt gtagggattc gcttacatac
       61 acctttacat cccccgtcat tttcaatcca gagtaaattt acccccgttc tgtacagaat
      121 atccagtaca ttttccgaat ttaccgcttt gcgggaattg aactcttttc gcttcatatc
      181 tgaaaacatg catggaacag atatggctgt ggatgtgcca cacgactgca cattatgaaa
      241 ggatataagt tcattatatt tctttgtaaa atcattggta ccacgagaat atccattcag
      301 agcatagttt tgacttcttg ctgtctcccc gacaactaaa aacataatta ccggcttttg
      361 tttttcattt attgtgcggg atgcatccgt tccagtctga cgaaactcgc caggagaaac
      421 aaaaaaatcc cttacatatt taaacccact gtagatataa ttagtaggta ttattt
//
LOCUS       DAZKHF020000792          476 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2201S31241
            SAMN43267800-rid25106413.denovo.796, whole genome shotgun sequence.
ACCESSION   DAZKHF020000792 DAZKHF020000000
VERSION     DAZKHF020000792.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267800
            Sequence Read Archive: SRR30307263
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 476)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 476)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 476)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350581.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350581.2
            Genome Coverage       :: 151x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:21:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,652
            CDSs (total)                      :: 5,557
            Genes (coding)                    :: 5,320
            CDSs (with protein)               :: 5,320
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 237
            CDSs (without protein)            :: 237
            Pseudo Genes (ambiguous residues) :: 0 of 237
            Pseudo Genes (frameshifted)       :: 102 of 237
            Pseudo Genes (incomplete)         :: 130 of 237
            Pseudo Genes (internal stop)      :: 52 of 237
            Pseudo Genes (multiple problems)  :: 41 of 237
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..476
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267800-rid25106413.denovo.796"
                     /serotype="O 26:H11"
                     /isolate="2201S31241"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-01"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            <1..>476
                     /locus_tag="ACHOSC_RS27500"
                     /old_locus_tag="pgaptmp_005500"
     rRNA            <1..>476
                     /locus_tag="ACHOSC_RS27500"
                     /old_locus_tag="pgaptmp_005500"
                     /product="16S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00177"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00177"
BASE COUNT          119 a          115 c          142 g          100 t
ORIGIN      
        1 tgtcgtcagc tcgtgttgtg aaatgttggg ttaagtcccg caacgagcgc aacccttatc
       61 ctttgttgcc agcggtccgg ccgggaactc aaaggagact gccagtgata aactggagga
      121 aggtggggat gacgtcaagt catcatggcc cttacgacca gggctacaca cgtgctacaa
      181 tggcgcatac aaagagaagc gacctcgcga gagcaagcgg acctcataaa gtgcgtcgta
      241 gtccggattg gagtctgcaa ctcgactcca tgaagtcgga atcgctagta atcgtggatc
      301 agaatgccac ggtgaatacg ttcccgggcc ttgtacacac cgcccgtcac accatgggag
      361 tgggttgcaa aagaagtagg tagcttaacc ttcgggaggg cgcttaccac tttgtgattc
      421 atgactgggg tgaagtcgta acaaggtaac cgtaggggaa cctgcggttg gatcac
//
LOCUS       DAZKHF020000793          474 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2201S31241
            SAMN43267800-rid25106413.denovo.797, whole genome shotgun sequence.
ACCESSION   DAZKHF020000793 DAZKHF020000000
VERSION     DAZKHF020000793.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267800
            Sequence Read Archive: SRR30307263
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 474)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 474)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 474)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350581.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350581.2
            Genome Coverage       :: 151x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:21:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,652
            CDSs (total)                      :: 5,557
            Genes (coding)                    :: 5,320
            CDSs (with protein)               :: 5,320
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 237
            CDSs (without protein)            :: 237
            Pseudo Genes (ambiguous residues) :: 0 of 237
            Pseudo Genes (frameshifted)       :: 102 of 237
            Pseudo Genes (incomplete)         :: 130 of 237
            Pseudo Genes (internal stop)      :: 52 of 237
            Pseudo Genes (multiple problems)  :: 41 of 237
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..474
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267800-rid25106413.denovo.797"
                     /serotype="O 26:H11"
                     /isolate="2201S31241"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-01"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            <1..276
                     /locus_tag="ACHOSC_RS27505"
                     /old_locus_tag="pgaptmp_005501"
     CDS             <1..276
                     /locus_tag="ACHOSC_RS27505"
                     /old_locus_tag="pgaptmp_005501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001365813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PrgH/EprH family type III secretion apparatus
                     protein"
                     /protein_id="HGU4551200.1"
                     /translation="ESQAIETLSAIGLSYEKYKTANNVYFNIIGTLSDSELNKINNYV
                     DEYYKQWGKQYVRFNVNLKNQDTNNSSFSYGDNRFEKSQGSNWTFQE"
     gene            290..474
                     /gene="sctF"
                     /locus_tag="ACHOSC_RS27510"
                     /old_locus_tag="pgaptmp_005502"
                     /pseudo
     CDS             290..474
                     /gene="sctF"
                     /locus_tag="ACHOSC_RS27510"
                     /old_locus_tag="pgaptmp_005502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311745.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_component: GO:0030257 - type III protein secretion
                     system complex [Evidence IEA];
                     GO_process: GO:0030254 - protein secretion by the type III
                     secretion system [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system needle filament
                     subunit SctF"
BASE COUNT          175 a           67 c           97 g          135 t
ORIGIN      
        1 gaaagtcagg ctattgaaac gttgtcagcg ataggtcttt cttatgaaaa atataaaaca
       61 gctaacaatg tgtatttcaa tatcattggt acgttaagtg acagtgaatt aaataaaatt
      121 aataactatg ttgacgaata ttataaacaa tggggtaaac aatatgtaag atttaatgtg
      181 aatttgaaaa atcaggacac aaataatagt tcatttagct acggagataa ccgattcgag
      241 aagtctcaag gtagcaactg gacatttcag gaataattga ggaggactta tggcagattg
      301 gaatggttat attatggata tcagcaatca atttgatcag ggcgttgatg atctgaacca
      361 gcaagttgaa aaagcgttgg aggatttagc caaccaatcc ctccgacacg aaatttcttg
      421 ccgaatatca aagtgcatta gctgagtata cattatatcg aaatgcgcaa tcta
//
LOCUS       DAZKHF020000794          473 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2201S31241
            SAMN43267800-rid25106413.denovo.798, whole genome shotgun sequence.
ACCESSION   DAZKHF020000794 DAZKHF020000000
VERSION     DAZKHF020000794.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267800
            Sequence Read Archive: SRR30307263
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 473)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 473)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 473)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350581.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350581.2
            Genome Coverage       :: 151x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:21:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,652
            CDSs (total)                      :: 5,557
            Genes (coding)                    :: 5,320
            CDSs (with protein)               :: 5,320
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 237
            CDSs (without protein)            :: 237
            Pseudo Genes (ambiguous residues) :: 0 of 237
            Pseudo Genes (frameshifted)       :: 102 of 237
            Pseudo Genes (incomplete)         :: 130 of 237
            Pseudo Genes (internal stop)      :: 52 of 237
            Pseudo Genes (multiple problems)  :: 41 of 237
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..473
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267800-rid25106413.denovo.798"
                     /serotype="O 26:H11"
                     /isolate="2201S31241"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-01"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            <1..432
                     /locus_tag="ACHOSC_RS27515"
                     /old_locus_tag="pgaptmp_005503"
     CDS             <1..432
                     /locus_tag="ACHOSC_RS27515"
                     /old_locus_tag="pgaptmp_005503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4551201.1"
                     /translation="IPELSALPPSLRKKNFTDAYNAASSHIEYWIGAGVSFISMMILS
                     RVYDFLLPAQDTFPGDIIRSLCVVCPSILIWFQFSVYVMRKYYRHILVRGKETETISE
                     RLIREADTREYELWRPVRRFFSIVFLIVLLGCIHSLITTIK"
BASE COUNT          136 a           91 c           87 g          159 t
ORIGIN      
        1 attcctgaac tttcagcact tccaccttca ttaagaaaga aaaattttac tgacgcatat
       61 aatgcagcat cttctcatat cgaatactgg ataggtgctg gcgtctcatt tatctcgatg
      121 atgattttat ccagagtata tgactttctt ttacccgccc aagatacttt cccgggtgat
      181 atcatcagaa gtttatgtgt tgtgtgtcca tccatattaa tctggtttca gttttcggta
      241 tatgtcatga ggaaatatta cagacatatt ctggtgcgag gaaaagaaac cgaaaccatt
      301 tcagaaaggt tgatacgtga ggctgataca cgagaatatg aactatggcg tccggtccgt
      361 cgctttttct ccattgtttt cctcattgtt ctattaggtt gcattcattc actgataaca
      421 actataaaat aaaagttatg gctgttctga tacaggaaaa taaacgttca ttc
//
LOCUS       DAZKHF020000795          473 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2201S31241
            SAMN43267800-rid25106413.denovo.799, whole genome shotgun sequence.
ACCESSION   DAZKHF020000795 DAZKHF020000000
VERSION     DAZKHF020000795.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267800
            Sequence Read Archive: SRR30307263
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 473)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 473)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 473)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350581.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350581.2
            Genome Coverage       :: 151x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:21:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,652
            CDSs (total)                      :: 5,557
            Genes (coding)                    :: 5,320
            CDSs (with protein)               :: 5,320
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 237
            CDSs (without protein)            :: 237
            Pseudo Genes (ambiguous residues) :: 0 of 237
            Pseudo Genes (frameshifted)       :: 102 of 237
            Pseudo Genes (incomplete)         :: 130 of 237
            Pseudo Genes (internal stop)      :: 52 of 237
            Pseudo Genes (multiple problems)  :: 41 of 237
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..473
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267800-rid25106413.denovo.799"
                     /serotype="O 26:H11"
                     /isolate="2201S31241"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-01"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            <1..>473
                     /locus_tag="ACHOSC_RS27520"
                     /old_locus_tag="pgaptmp_005504"
     CDS             <1..>473
                     /locus_tag="ACHOSC_RS27520"
                     /old_locus_tag="pgaptmp_005504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4551202.1"
                     /translation="NNRVYNPGGISGASLEGFISSGLEVCASRIGGTAGRYLSNVAKV
                     IKLPLLDIGINIWSLYDSSLNHARATTQIEYISTAIDVSFSSINTALSIGAIAYPPLA
                     IAIVPITIFSHEVKNYAVYVNQINERHKLWLEAEKYLDNGSAKVLSINKDTGIID"
BASE COUNT          156 a           82 c           89 g          146 t
ORIGIN      
        1 taataatcgc gtatataacc ctggtggtat atctggtgcc tcgcttgaag gatttatatc
       61 ttctggttta gaagtgtgcg cgtcaagaat aggtggcacc gctgggcgtt atcttagcaa
      121 tgtggcaaaa gtaataaaat tacctttgct agatataggc ataaatattt ggtcattata
      181 tgactcttca ttaaatcatg ccagagccac cacacaaatt gaatatatat caacagcgat
      241 agatgtatct ttttcatcta taaatactgc tttatcaata ggcgcaatcg catatccacc
      301 tttggcaata gctatagttc cgataactat cttttctcat gaagtaaaaa actatgctgt
      361 ttatgtgaat caaattaatg agcgacataa gttatggctt gaagctgaaa aatatctgga
      421 caatggaagc gcaaaggttc taagcattaa caaagataca ggcataatcg att
//
LOCUS       DAZKHF020000796          472 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2201S31241
            SAMN43267800-rid25106413.denovo.800, whole genome shotgun sequence.
ACCESSION   DAZKHF020000796 DAZKHF020000000
VERSION     DAZKHF020000796.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267800
            Sequence Read Archive: SRR30307263
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 472)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 472)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 472)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350581.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350581.2
            Genome Coverage       :: 151x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:21:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,652
            CDSs (total)                      :: 5,557
            Genes (coding)                    :: 5,320
            CDSs (with protein)               :: 5,320
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 237
            CDSs (without protein)            :: 237
            Pseudo Genes (ambiguous residues) :: 0 of 237
            Pseudo Genes (frameshifted)       :: 102 of 237
            Pseudo Genes (incomplete)         :: 130 of 237
            Pseudo Genes (internal stop)      :: 52 of 237
            Pseudo Genes (multiple problems)  :: 41 of 237
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..472
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267800-rid25106413.denovo.800"
                     /serotype="O 26:H11"
                     /isolate="2201S31241"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-01"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            complement(<1..183)
                     /locus_tag="ACHOSC_RS27525"
                     /old_locus_tag="pgaptmp_005505"
     CDS             complement(<1..183)
                     /locus_tag="ACHOSC_RS27525"
                     /old_locus_tag="pgaptmp_005505"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4551203.1"
                     /translation="MKHHPENTNINKPLQRDFRSDQLQATNQETYFMMRIMLSLMAGK
                     HLFSPRPLSYLANLSLF"
     gene            complement(259..>472)
                     /locus_tag="ACHOSC_RS27530"
                     /old_locus_tag="pgaptmp_005506"
     CDS             complement(259..>472)
                     /locus_tag="ACHOSC_RS27530"
                     /old_locus_tag="pgaptmp_005506"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4551204.1"
                     /translation="YDIIRQLHLPQNGYFMERSNFLVAIIRLILRSLAIIPGWFVSLV
                     HEIVTFLFILEIWGKAGDDFFICIRW"
BASE COUNT          142 a           90 c          107 g          133 t
ORIGIN      
        1 aaacaaggaa agatttgcaa gataactcag tgggcgtggt gaaaataagt gtttacccgc
       61 catcaggctg agcataattc tcatcatgaa atatgtttcc tggtttgtgg cttgtagctg
      121 gtcactcctg aagtctctct ggagaggctt gttgatgttg gtgttttcag gatgatgttt
      181 cacttagctt gtttgccgaa tagcccggcg aacagctgtg attgaggaag gtttaagtcg
      241 cagtgaccaa agccatattt accaacggat gcagatgaaa aaatcatctc ctgctttccc
      301 ccatatctcc aggataaaaa ggaatgtaac aatctcatga actaaactga cgaaccagcc
      361 aggaataatc gctagggacc taagaattag cctgataata gctactaaga aattactgcg
      421 ttccatgaaa tagccatttt gtggcaaatg gagttgtcta ataatgtcat at
//
LOCUS       DAZKHF020000797          472 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2201S31241
            SAMN43267800-rid25106413.denovo.801, whole genome shotgun sequence.
ACCESSION   DAZKHF020000797 DAZKHF020000000
VERSION     DAZKHF020000797.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267800
            Sequence Read Archive: SRR30307263
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 472)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 472)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 472)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350581.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350581.2
            Genome Coverage       :: 151x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:21:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,652
            CDSs (total)                      :: 5,557
            Genes (coding)                    :: 5,320
            CDSs (with protein)               :: 5,320
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 237
            CDSs (without protein)            :: 237
            Pseudo Genes (ambiguous residues) :: 0 of 237
            Pseudo Genes (frameshifted)       :: 102 of 237
            Pseudo Genes (incomplete)         :: 130 of 237
            Pseudo Genes (internal stop)      :: 52 of 237
            Pseudo Genes (multiple problems)  :: 41 of 237
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..472
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267800-rid25106413.denovo.801"
                     /serotype="O 26:H11"
                     /isolate="2201S31241"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-01"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            complement(<1..>472)
                     /gene="nleC"
                     /locus_tag="ACHOSC_RS27535"
                     /old_locus_tag="pgaptmp_005507"
     CDS             complement(<1..>472)
                     /gene="nleC"
                     /locus_tag="ACHOSC_RS27535"
                     /old_locus_tag="pgaptmp_005507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308874.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="type III secretion system effector zinc
                     metalloprotease NleC"
                     /protein_id="HGU4551205.1"
                     /translation="RLIDQHTVDMIGNTVLDALSRSQTFRDAVSYGIHNEEVHIGCIK
                     YRNEYELNGESAIKIDDIQSLTCNELYGYDVGQEPILPICEAGENENEEPYVSFSVAP
                     DTDSYEMPSWQEGLIHEIIHHVTGASDPSGDSNIELGPTEILARRVAQELGWSVP"
BASE COUNT          120 a          117 c           96 g          139 t
ORIGIN      
        1 ggaacactcc atcccagctc ttgagcgaca cgacgtgcga gaatctcggt gggtcctagc
       61 tctatattac tatctccaga tggatcgctg gccccagtaa catgatgaat aatctcgtga
      121 atcagtcctt cctgccacga tggcatctca taagaatcag tatctggcgc aacactaaaa
      181 ctgacataag gctcttcgtt ctcgttttct cctgcctcgc aaatggggag aattggttct
      241 tgcccgacat cgtatccata taattcgtta caggttagtg attgaatatc atcaattttg
      301 atagcagatt ctccgttaag ctcgtattcg tttctgtatt taatgcaacc aatgtgtacc
      361 tcctcattat gaatgccata gcttacggca tcacgaaatg tttgtgatcg gctcaacgca
      421 tcaagtacag tgttgccaat catatcgacc gtatgctgat cgatgagcct gc
//
LOCUS       DAZKHF020000798          468 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2201S31241
            SAMN43267800-rid25106413.denovo.802, whole genome shotgun sequence.
ACCESSION   DAZKHF020000798 DAZKHF020000000
VERSION     DAZKHF020000798.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267800
            Sequence Read Archive: SRR30307263
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 468)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 468)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 468)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350581.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350581.2
            Genome Coverage       :: 151x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:21:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,652
            CDSs (total)                      :: 5,557
            Genes (coding)                    :: 5,320
            CDSs (with protein)               :: 5,320
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 237
            CDSs (without protein)            :: 237
            Pseudo Genes (ambiguous residues) :: 0 of 237
            Pseudo Genes (frameshifted)       :: 102 of 237
            Pseudo Genes (incomplete)         :: 130 of 237
            Pseudo Genes (internal stop)      :: 52 of 237
            Pseudo Genes (multiple problems)  :: 41 of 237
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..468
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267800-rid25106413.denovo.802"
                     /serotype="O 26:H11"
                     /isolate="2201S31241"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-01"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            1..468
                     /locus_tag="ACHOSC_RS27540"
                     /old_locus_tag="pgaptmp_005508"
                     /pseudo
     CDS             1..468
                     /locus_tag="ACHOSC_RS27540"
                     /old_locus_tag="pgaptmp_005508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308587.2"
                     /note="frameshifted; incomplete; missing N-terminus and
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="RHS repeat-associated core domain-containing
                     protein"
BASE COUNT          147 a           74 c          125 g          122 t
ORIGIN      
        1 aaccggaacc ggtactacga tccgttgcag gggcggtata tcactcagga cccgataggg
       61 ctggaggggg ggatggagtc tgtatgcgta cccgctgaat ccggtgaatg gtattgatcc
      121 attagggtta agtcccgcag atgtagcgct aataagaaga aaagatcaac taaaccatca
      181 aagagcatgg gatatattat ctgatactta tgaagatatg aagagattaa atttaggtgg
      241 gactgatcaa tttttccatt gtatggcatt ttgtcgagtg tctaaattaa atgacgctgg
      301 tgttagccga tcggcgaaag ggctgggtta tgaaaaagag attagagatt acgggttaaa
      361 tctgttcggt atgtacggca gaaaagtaaa gctatcccat tctgaaatga ttgaagataa
      421 taaaaaagac ttggctgtaa atgaccatgg gttgacatgt ccatcaac
//
LOCUS       DAZKHF020000799          467 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2201S31241
            SAMN43267800-rid25106413.denovo.803, whole genome shotgun sequence.
ACCESSION   DAZKHF020000799 DAZKHF020000000
VERSION     DAZKHF020000799.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267800
            Sequence Read Archive: SRR30307263
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 467)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 467)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 467)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350581.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350581.2
            Genome Coverage       :: 151x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:21:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,652
            CDSs (total)                      :: 5,557
            Genes (coding)                    :: 5,320
            CDSs (with protein)               :: 5,320
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 237
            CDSs (without protein)            :: 237
            Pseudo Genes (ambiguous residues) :: 0 of 237
            Pseudo Genes (frameshifted)       :: 102 of 237
            Pseudo Genes (incomplete)         :: 130 of 237
            Pseudo Genes (internal stop)      :: 52 of 237
            Pseudo Genes (multiple problems)  :: 41 of 237
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..467
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267800-rid25106413.denovo.803"
                     /serotype="O 26:H11"
                     /isolate="2201S31241"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-01"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            106..>467
                     /locus_tag="ACHOSC_RS27545"
                     /old_locus_tag="pgaptmp_005509"
     CDS             106..>467
                     /locus_tag="ACHOSC_RS27545"
                     /old_locus_tag="pgaptmp_005509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Arm DNA-binding domain-containing protein"
                     /protein_id="HGU4551206.1"
                     /translation="MALTDAKIRAAKPTDKAYKLTDGAGMFLLVHPNGSRYWRLRYRI
                     LGKEKTLALGVYPEVSLSEARTKRDEARKLISEGVDPCEQKRAKKVVPDLQLSFEHIA
                     RRWHASNKQWAQSHSDKVL"
BASE COUNT          133 a          117 c          105 g          112 t
ORIGIN      
        1 caaccaaatt ctccccacat caacctcatt gtgtgggtat aattgcgggc ataccccagt
       61 tcgacagaat ttgtaccctc tttaacgcat tcagaaggct gaactatggc actgactgac
      121 gcaaaaatcc gggctgcaaa gcccactgac aaggcttata aactcactga cggggctggc
      181 atgttcctgc tggtacatcc taatggttcc cgttactggc gtctccgtta tcgtattctg
      241 ggtaaagaga agactctggc acttggtgtg tatccagaag tatctctctc cgaagctcgt
      301 acaaaacggg atgaggcccg aaaactgatt tcggaggggg ttgacccttg cgaacagaaa
      361 agagctaaaa aagtagtccc tgatttacaa ctctctttcg aacatattgc acgacgctgg
      421 catgccagta ataaacaatg ggcacaatca cacagcgata aagtact
//
LOCUS       DAZKHF020000800          465 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2201S31241
            SAMN43267800-rid25106413.denovo.804, whole genome shotgun sequence.
ACCESSION   DAZKHF020000800 DAZKHF020000000
VERSION     DAZKHF020000800.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267800
            Sequence Read Archive: SRR30307263
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 465)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 465)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 465)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350581.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350581.2
            Genome Coverage       :: 151x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:21:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,652
            CDSs (total)                      :: 5,557
            Genes (coding)                    :: 5,320
            CDSs (with protein)               :: 5,320
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 237
            CDSs (without protein)            :: 237
            Pseudo Genes (ambiguous residues) :: 0 of 237
            Pseudo Genes (frameshifted)       :: 102 of 237
            Pseudo Genes (incomplete)         :: 130 of 237
            Pseudo Genes (internal stop)      :: 52 of 237
            Pseudo Genes (multiple problems)  :: 41 of 237
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..465
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267800-rid25106413.denovo.804"
                     /serotype="O 26:H11"
                     /isolate="2201S31241"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-01"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            complement(81..326)
                     /locus_tag="ACHOSC_RS27550"
                     /old_locus_tag="pgaptmp_005510"
     CDS             complement(81..326)
                     /locus_tag="ACHOSC_RS27550"
                     /old_locus_tag="pgaptmp_005510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF826 domain-containing protein"
                     /protein_id="HGU4551207.1"
                     /translation="MSEITSLVTAEAVKEVLRSEEVRSALKQKLRHNLEARLDAEVDA
                     ILDELLGAPAAPEPEGIAGEGSASDSGDPTPDSDMMM"
     gene            complement(367..>465)
                     /locus_tag="ACHOSC_RS27555"
                     /old_locus_tag="pgaptmp_005511"
     CDS             complement(367..>465)
                     /locus_tag="ACHOSC_RS27555"
                     /old_locus_tag="pgaptmp_005511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1378 family protein"
                     /protein_id="HGU4551208.1"
                     /translation="VRDCWRRQIDKRAAEKISAGQSAGSKPEEPLI"
BASE COUNT           93 a          146 c          114 g          112 t
ORIGIN      
        1 cataccccgc ctcccgcaat ccggaagctc acaacaataa aaaagaccac cggcacacac
       61 cgatggtccc tgacgcatgc ttacatcatc atgtcgctgt ccggtgtggg gtcaccgcta
      121 tctgaagcac tcccctcacc cgcgatgcct tccggctccg gagctgccgg tgcgcccagc
      181 agttcatcca gaatggcatc cacttctgca tcaagacgcg cttccaggtt atggcgaagt
      241 ttctgtttca gtgcgctccg gacttcttca gagcgcagga cttccttcac tgcttcagca
      301 gtgaccaggg atgtaatttc tgacatggga ttttctcgtc gaaaggtgtg attaagaaag
      361 ttgccgctaa atgagcggct cttcgggttt gcttccggct gactgaccgg cgctgatttt
      421 ctcagcggcc cttttgtcaa tctgcctgcg ccagcaatcg cgcac
//
LOCUS       DAZKHF020000801          458 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2201S31241
            SAMN43267800-rid25106413.denovo.806, whole genome shotgun sequence.
ACCESSION   DAZKHF020000801 DAZKHF020000000
VERSION     DAZKHF020000801.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267800
            Sequence Read Archive: SRR30307263
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 458)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 458)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 458)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350581.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350581.2
            Genome Coverage       :: 151x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:21:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,652
            CDSs (total)                      :: 5,557
            Genes (coding)                    :: 5,320
            CDSs (with protein)               :: 5,320
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 237
            CDSs (without protein)            :: 237
            Pseudo Genes (ambiguous residues) :: 0 of 237
            Pseudo Genes (frameshifted)       :: 102 of 237
            Pseudo Genes (incomplete)         :: 130 of 237
            Pseudo Genes (internal stop)      :: 52 of 237
            Pseudo Genes (multiple problems)  :: 41 of 237
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..458
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267800-rid25106413.denovo.806"
                     /serotype="O 26:H11"
                     /isolate="2201S31241"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-01"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            complement(168..387)
                     /locus_tag="ACHOSC_RS27560"
                     /old_locus_tag="pgaptmp_005512"
                     /pseudo
     CDS             complement(168..387)
                     /locus_tag="ACHOSC_RS27560"
                     /old_locus_tag="pgaptmp_005512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015869530.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="transposase"
BASE COUNT          124 a           87 c          114 g          133 t
ORIGIN      
        1 taaaaggaga gggtattgtc tggacatttc tgttagcata attggaaatg tgcgtgaaaa
       61 aaagcataac agacatcatg tgttatgtat gtattcagag tgcccataat tcaccagtgc
      121 agggataaat tgtaaaaaaa tcgcagtacg tcgtatgcag aacggtgcgg cgtacgcagt
      181 tccagtggac cgtcgcctgt gataacggtc tttgtggaat aaccgttgcg ggagttagct
      241 cctgatctgg actgattttt ctcattccca agatggtgtg tcatctctgc attgagagcg
      301 gcttcaacgc tgagcttttt cagcaaccga tcaaactgac tgaggtcttc tgagggggct
      361 gaggtttttg gccagttcgt tagcaataca ccagaaaaat tctaaacgag cctgtacata
      421 gatttgtgta attgcctgat tttgatatgt tcaaccca
//
LOCUS       DAZKHF020000802          457 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2201S31241
            SAMN43267800-rid25106413.denovo.807, whole genome shotgun sequence.
ACCESSION   DAZKHF020000802 DAZKHF020000000
VERSION     DAZKHF020000802.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267800
            Sequence Read Archive: SRR30307263
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 457)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 457)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 457)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350581.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350581.2
            Genome Coverage       :: 151x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:21:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,652
            CDSs (total)                      :: 5,557
            Genes (coding)                    :: 5,320
            CDSs (with protein)               :: 5,320
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 237
            CDSs (without protein)            :: 237
            Pseudo Genes (ambiguous residues) :: 0 of 237
            Pseudo Genes (frameshifted)       :: 102 of 237
            Pseudo Genes (incomplete)         :: 130 of 237
            Pseudo Genes (internal stop)      :: 52 of 237
            Pseudo Genes (multiple problems)  :: 41 of 237
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..457
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267800-rid25106413.denovo.807"
                     /serotype="O 26:H11"
                     /isolate="2201S31241"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-01"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            complement(<1..203)
                     /locus_tag="ACHOSC_RS27565"
                     /old_locus_tag="pgaptmp_005513"
     CDS             complement(<1..203)
                     /locus_tag="ACHOSC_RS27565"
                     /old_locus_tag="pgaptmp_005513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tube protein"
                     /protein_id="HGU4551209.1"
                     /translation="MPTPNPLAPVKGAGTTLWVYNGQGDAYANPLSDDNWQRLAQVKD
                     LTPGEMTAEPYDDNYLDDEDADWT"
     gene            complement(211..>457)
                     /locus_tag="ACHOSC_RS27570"
                     /old_locus_tag="pgaptmp_005514"
     CDS             complement(211..>457)
                     /locus_tag="ACHOSC_RS27570"
                     /old_locus_tag="pgaptmp_005514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="phage minor tail U family protein"
                     /protein_id="HGU4551210.1"
                     /translation="EALDSDTWQAELHIEVFLPAQVPDSELDSWMESRIYPAMSAIPA
                     LSGLITTMVTQGYEYRRDDDMALWSSADLTYSITYEM"
BASE COUNT          102 a          138 c          106 g          111 t
ORIGIN      
        1 gtccagtccg cgtcttcatc atccaggtag ttatcatcgt agggttctgc cgtcatctcg
       61 cccggcgtca gatcctttac ctgtgccagt cgctgccagt tatcgtctga caacgggttt
      121 gcataggcat caccctgacc gttataaacc cacagggtgg taccggcacc ttttaccggt
      181 gccagaggat ttggtgttgg catatcgtcc tcacatctcg tatgtaatgg aataagtcaa
      241 atcagcagaa ctccataacg ccatatcgtc atcacgacga tactcatagc cctgcgtaac
      301 catcgtggta atcaggcctg acagtgccgg gatcgcactc atcgccggat aaatccggct
      361 ttccatccac gaatccagct ctgaatccgg cacctgagca ggcaggaaaa cttcaatatg
      421 cagctccgcc tgccaggtat cgctgtccag cgcttcg
//
LOCUS       DAZKHF020000803          457 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2201S31241
            SAMN43267800-rid25106413.denovo.808, whole genome shotgun sequence.
ACCESSION   DAZKHF020000803 DAZKHF020000000
VERSION     DAZKHF020000803.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267800
            Sequence Read Archive: SRR30307263
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 457)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 457)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 457)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350581.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350581.2
            Genome Coverage       :: 151x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:21:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,652
            CDSs (total)                      :: 5,557
            Genes (coding)                    :: 5,320
            CDSs (with protein)               :: 5,320
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 237
            CDSs (without protein)            :: 237
            Pseudo Genes (ambiguous residues) :: 0 of 237
            Pseudo Genes (frameshifted)       :: 102 of 237
            Pseudo Genes (incomplete)         :: 130 of 237
            Pseudo Genes (internal stop)      :: 52 of 237
            Pseudo Genes (multiple problems)  :: 41 of 237
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..457
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267800-rid25106413.denovo.808"
                     /serotype="O 26:H11"
                     /isolate="2201S31241"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-01"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
BASE COUNT          146 a           72 c          106 g          133 t
ORIGIN      
        1 tttagaggaa ataattttgt ctggttcata aattcatctt ctttgttgag aagattaact
       61 gcctaggtga aattatcctc tatgaccggg acgctgcagg ccacctggca gaagtcatca
      121 cgcaggaaga caagtcgctg tatccctttg aaaaatccgg caggctaatg ggcatcttca
      181 gcccaggcgg cacatcacag cgaatttgaa ggcaaatgcg aaaattagtg atcttgaagt
      241 tcatacagta ttggtaaaaa caggcgaagc ttttccaatg tgtaagaatt ttcaaatttc
      301 aatatttggc gctgttgtta caagcgggtg attatatgtg tgaattaatt aatgaagcat
      361 caaaatatgg gaaagtcaat gaaagaaaga tggacattga tttgtgctgt aaaaaaatag
      421 aggaagaagg atattctgtt ttcgttgcgt tctatga
//
LOCUS       DAZKHF020000804          454 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2201S31241
            SAMN43267800-rid25106413.denovo.809, whole genome shotgun sequence.
ACCESSION   DAZKHF020000804 DAZKHF020000000
VERSION     DAZKHF020000804.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267800
            Sequence Read Archive: SRR30307263
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 454)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 454)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 454)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350581.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350581.2
            Genome Coverage       :: 151x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:21:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,652
            CDSs (total)                      :: 5,557
            Genes (coding)                    :: 5,320
            CDSs (with protein)               :: 5,320
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 237
            CDSs (without protein)            :: 237
            Pseudo Genes (ambiguous residues) :: 0 of 237
            Pseudo Genes (frameshifted)       :: 102 of 237
            Pseudo Genes (incomplete)         :: 130 of 237
            Pseudo Genes (internal stop)      :: 52 of 237
            Pseudo Genes (multiple problems)  :: 41 of 237
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..454
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267800-rid25106413.denovo.809"
                     /serotype="O 26:H11"
                     /isolate="2201S31241"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-01"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            1..454
                     /locus_tag="ACHOSC_RS27575"
                     /old_locus_tag="pgaptmp_005515"
                     /pseudo
     CDS             1..454
                     /locus_tag="ACHOSC_RS27575"
                     /old_locus_tag="pgaptmp_005515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611997.1"
                     /note="frameshifted; incomplete; missing N-terminus and
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3-like element ISSfl10 family transposase"
BASE COUNT          140 a          107 c          116 g           91 t
ORIGIN      
        1 cgtaaggcgt taaatgaagc tcgccttgag cgagatatat taaaaaaagc aacagcgtat
       61 tttgcacagg agtcgctgaa aaatacgcgt taatcgaaca atggcgacaa caatttccca
      121 ttgaagcgat gtgtcaggta tttggtgtat ccaggagcgg ttattacaac tgggtacagc
      181 atgaaccctc agacagaaaa caaagtgatg agcggctaaa actggagatt aaggtggcac
      241 atatccgcac tcgcgaaaca tatggaaccc ggcggctcca gacggagctg gcagagaatg
      301 gcatcatcgt tggtcgtgac cgactggcac gtcttcgtaa ggagctaagg ctacgctgta
      361 agcagaaacg caagttcaga gcgactacga actcgaacca caatctgcca gttgcgccaa
      421 atctgctgaa ccagacgttc gctcctacag cacc
//
LOCUS       DAZKHF020000805          454 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2201S31241
            SAMN43267800-rid25106413.denovo.810, whole genome shotgun sequence.
ACCESSION   DAZKHF020000805 DAZKHF020000000
VERSION     DAZKHF020000805.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267800
            Sequence Read Archive: SRR30307263
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 454)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 454)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 454)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350581.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350581.2
            Genome Coverage       :: 151x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:21:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,652
            CDSs (total)                      :: 5,557
            Genes (coding)                    :: 5,320
            CDSs (with protein)               :: 5,320
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 237
            CDSs (without protein)            :: 237
            Pseudo Genes (ambiguous residues) :: 0 of 237
            Pseudo Genes (frameshifted)       :: 102 of 237
            Pseudo Genes (incomplete)         :: 130 of 237
            Pseudo Genes (internal stop)      :: 52 of 237
            Pseudo Genes (multiple problems)  :: 41 of 237
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..454
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267800-rid25106413.denovo.810"
                     /serotype="O 26:H11"
                     /isolate="2201S31241"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-01"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            complement(<1..86)
                     /locus_tag="ACHOSC_RS27580"
                     /old_locus_tag="pgaptmp_005516"
     CDS             complement(<1..86)
                     /locus_tag="ACHOSC_RS27580"
                     /old_locus_tag="pgaptmp_005516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EscT/YscT/HrcT family type III secretion system
                     export apparatus protein"
                     /protein_id="HGU4551211.1"
                     /translation="MNEIMTVIVSSFFCILRPLGMFIILPIFS"
     gene            complement(86..355)
                     /gene="escS"
                     /locus_tag="ACHOSC_RS27585"
                     /old_locus_tag="pgaptmp_005517"
     CDS             complement(86..355)
                     /gene="escS"
                     /locus_tag="ACHOSC_RS27585"
                     /old_locus_tag="pgaptmp_005517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system LEE export apparatus
                     protein EscS"
                     /protein_id="HGU4551212.1"
                     /translation="METGYFVQLCVQTLWIIFILSLPTVIAASVVGIIISLVQAITQL
                     QDQTLPFLLKIIAVFATLALTYHWMGTTIINFSSIIFEMIPKVNG"
     gene            complement(355..>454)
                     /locus_tag="ACHOSC_RS27590"
                     /old_locus_tag="pgaptmp_005518"
     CDS             complement(355..>454)
                     /locus_tag="ACHOSC_RS27590"
                     /old_locus_tag="pgaptmp_005518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312610.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="EscR/YscR/HrcR family type III secretion system
                     export apparatus protein"
                     /protein_id="HGU4551213.1"
                     /translation="VSPVTISIPFKILLFILVGGWQKLFEFLLVVN"
BASE COUNT          163 a           90 c           80 g          121 t
ORIGIN      
        1 gaaaatattg gtagaataat aaacatcccc agcggcctga gtatacaaaa aaatgatgat
       61 actatgaccg tcattatttc attcattagc cattcacctt cggaatcatt tcaaatatta
      121 tcgacgagaa gttgatgatt gttgttccca tccagtgata agtcagggca agcgtagcaa
      181 acactgctat tatttttagc aaaaaaggca atgtttgatc ctgtaactgc gttatagcct
      241 gaaccaggct aataataatg ccgacaacag aggccgctat gactgtaggc agtgagagga
      301 taaatattat ccaaagcgtt tgcacacata attgaacaaa atatccagtt tccatcaatt
      361 caccaccaac agaaattcga ataatttttg ccagcctcca acaagaatga ataacaagat
      421 cttaaaagga atcgaaattg ttactggcga tacc
//
LOCUS       DAZKHF020000806          453 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2201S31241
            SAMN43267800-rid25106413.denovo.811, whole genome shotgun sequence.
ACCESSION   DAZKHF020000806 DAZKHF020000000
VERSION     DAZKHF020000806.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267800
            Sequence Read Archive: SRR30307263
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 453)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 453)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 453)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350581.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350581.2
            Genome Coverage       :: 151x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:21:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,652
            CDSs (total)                      :: 5,557
            Genes (coding)                    :: 5,320
            CDSs (with protein)               :: 5,320
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 237
            CDSs (without protein)            :: 237
            Pseudo Genes (ambiguous residues) :: 0 of 237
            Pseudo Genes (frameshifted)       :: 102 of 237
            Pseudo Genes (incomplete)         :: 130 of 237
            Pseudo Genes (internal stop)      :: 52 of 237
            Pseudo Genes (multiple problems)  :: 41 of 237
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..453
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267800-rid25106413.denovo.811"
                     /serotype="O 26:H11"
                     /isolate="2201S31241"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-01"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            <1..>453
                     /locus_tag="ACHOSC_RS27595"
                     /old_locus_tag="pgaptmp_005519"
     CDS             <1..>453
                     /locus_tag="ACHOSC_RS27595"
                     /old_locus_tag="pgaptmp_005519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="terminase gpA endonuclease subunit"
                     /protein_id="HGU4551214.1"
                     /translation="VRYTAAVPARVVYLTAGIDSQRNRFEMYVWGWAPGEEAFLVDKI
                     IIMGRPDEEETLLRVDAAINKKYRHADGTEMTISRVCWDTGGIDGEIVYQRSKKHGVF
                     RVLPVKGASVYGKPVITMPKTRNQRGVYLCEVGTDTAKEILYARMKADP"
BASE COUNT          110 a          101 c          142 g          100 t
ORIGIN      
        1 gtgcgttaca cggcggcggt gcctgcccgg gtggtttatc tgacggcggg cattgactcg
       61 cagcgaaacc gttttgagat gtatgtctgg ggatgggctc cgggagagga agcctttctg
      121 gtggataaaa tcatcattat ggggcgtcct gatgaggaag agacgctgtt acgtgtggat
      181 gcggcgatca acaaaaaata ccgccatgcg gatggcaccg aaatgactat ttcccgtgtc
      241 tgctgggaca ccggggggat cgatggtgaa attgtttatc agagatcaaa aaaacacggt
      301 gttttccggg tgctgccggt aaaaggcgca tctgtctatg gcaagccggt gatcaccatg
      361 ccaaaaaccc gcaatcagcg gggcgtgtat ctgtgtgaag tgggaacgga caccgcaaaa
      421 gaaattctct atgcccgtat gaaagccgat ccc
//
LOCUS       DAZKHF020000807          453 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2201S31241
            SAMN43267800-rid25106413.denovo.812, whole genome shotgun sequence.
ACCESSION   DAZKHF020000807 DAZKHF020000000
VERSION     DAZKHF020000807.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267800
            Sequence Read Archive: SRR30307263
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 453)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 453)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 453)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350581.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350581.2
            Genome Coverage       :: 151x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:21:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,652
            CDSs (total)                      :: 5,557
            Genes (coding)                    :: 5,320
            CDSs (with protein)               :: 5,320
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 237
            CDSs (without protein)            :: 237
            Pseudo Genes (ambiguous residues) :: 0 of 237
            Pseudo Genes (frameshifted)       :: 102 of 237
            Pseudo Genes (incomplete)         :: 130 of 237
            Pseudo Genes (internal stop)      :: 52 of 237
            Pseudo Genes (multiple problems)  :: 41 of 237
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..453
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267800-rid25106413.denovo.812"
                     /serotype="O 26:H11"
                     /isolate="2201S31241"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-01"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            <1..>453
                     /locus_tag="ACHOSC_RS27600"
                     /old_locus_tag="pgaptmp_005520"
     CDS             <1..>453
                     /locus_tag="ACHOSC_RS27600"
                     /old_locus_tag="pgaptmp_005520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="terminase gpA endonuclease subunit"
                     /protein_id="HGU4551215.1"
                     /translation="VRYTAAVPSRVVYLTAGIDSQRNRFEMYVWGWAPGEEAFLVDKI
                     IIMGRPDEEGTLLRVDAAINKKYRHADGTEMTISRVCWDTGGIDGEIVYQRSKKHGVF
                     RVLPVKGASVYGKPVITMPKTRNQRGVYLCEVGTDTAKEILYARMKADP"
BASE COUNT          108 a          103 c          142 g          100 t
ORIGIN      
        1 gtgcgttaca cggctgcggt gccttcccgg gtggtttatc tgacggcggg cattgactcg
       61 cagcgaaacc gttttgagat gtatgtctgg ggatgggcac cgggagagga agcctttctg
      121 gtggataaaa tcatcattat ggggcgtccc gatgaggaag ggacgctgtt acgtgtggat
      181 gctgcgatca acaaaaaata ccgccatgcg gatggcaccg aaatgaccat ttcccgtgtc
      241 tgctgggaca ccggggggat cgatggtgaa attgtttatc agagatcaaa aaaacacggt
      301 gttttccggg tgctgccggt aaaaggcgcg tctgtctatg gcaagccggt gatcaccatg
      361 ccgaaaaccc gcaatcagcg gggcgtgtat ctgtgtgaag tgggaacgga caccgcaaaa
      421 gaaattctct atgcccgtat gaaagccgat ccc
//
LOCUS       DAZKHF020000808          446 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2201S31241
            SAMN43267800-rid25106413.denovo.813, whole genome shotgun sequence.
ACCESSION   DAZKHF020000808 DAZKHF020000000
VERSION     DAZKHF020000808.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267800
            Sequence Read Archive: SRR30307263
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 446)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 446)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 446)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350581.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350581.2
            Genome Coverage       :: 151x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:21:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,652
            CDSs (total)                      :: 5,557
            Genes (coding)                    :: 5,320
            CDSs (with protein)               :: 5,320
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 237
            CDSs (without protein)            :: 237
            Pseudo Genes (ambiguous residues) :: 0 of 237
            Pseudo Genes (frameshifted)       :: 102 of 237
            Pseudo Genes (incomplete)         :: 130 of 237
            Pseudo Genes (internal stop)      :: 52 of 237
            Pseudo Genes (multiple problems)  :: 41 of 237
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..446
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267800-rid25106413.denovo.813"
                     /serotype="O 26:H11"
                     /isolate="2201S31241"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-01"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            complement(245..>446)
                     /locus_tag="ACHOSC_RS27605"
                     /old_locus_tag="pgaptmp_005521"
     CDS             complement(245..>446)
                     /locus_tag="ACHOSC_RS27605"
                     /old_locus_tag="pgaptmp_005521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000879781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4551216.1"
                     /translation="TLTVRAREQVAKIVSKNDPDTKKVWCKYGKIPGQGDGVNLFFVG
                     EINVTHYFITNIGAGLPDACAE"
BASE COUNT          130 a          104 c           64 g          148 t
ORIGIN      
        1 acacaatgtc agatatcgta aaagaccagt gcaattctat gtaaactctc tgactgttca
       61 ttttatttgt tgtttcaggg tcggaactca ccctcaataa acaactgaat acgagtagat
      121 attcatcgtc tgagctatat ggctttacac aatagccgac atgtcttttc tcagtcagga
      181 attttacttt tcctcactac gaagttgacg acttcgatat ggaatagact cttaattcaa
      241 gcaattactc tgcacaagca tcaggcaatc cagctccaat atttgttata aaataatgcg
      301 taacattaat ttcaccaaca aaaaaaaggt ttacaccatc cccttgccct ggtatcttac
      361 catatttaca ccacactttt tttgtatctg gatcattctt tgatacgatt ttcgccactt
      421 gttccctggc cctcactgtc aatgtt
//
LOCUS       DAZKHF020000809          445 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2201S31241
            SAMN43267800-rid25106413.denovo.814, whole genome shotgun sequence.
ACCESSION   DAZKHF020000809 DAZKHF020000000
VERSION     DAZKHF020000809.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267800
            Sequence Read Archive: SRR30307263
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 445)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 445)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 445)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350581.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350581.2
            Genome Coverage       :: 151x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:21:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,652
            CDSs (total)                      :: 5,557
            Genes (coding)                    :: 5,320
            CDSs (with protein)               :: 5,320
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 237
            CDSs (without protein)            :: 237
            Pseudo Genes (ambiguous residues) :: 0 of 237
            Pseudo Genes (frameshifted)       :: 102 of 237
            Pseudo Genes (incomplete)         :: 130 of 237
            Pseudo Genes (internal stop)      :: 52 of 237
            Pseudo Genes (multiple problems)  :: 41 of 237
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..445
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267800-rid25106413.denovo.814"
                     /serotype="O 26:H11"
                     /isolate="2201S31241"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-01"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
BASE COUNT          143 a           80 c          112 g          110 t
ORIGIN      
        1 aagataacta agatagataa gattaataag atcagaattc tctttcggtg tcgcctctgg
       61 cttttccacc gaaaacaatt acacgactac agaatgtagg aagtgaaatt gtattgaggc
      121 ttagtgaaat tacacgagga aagcagagtg taattgagct ttagccgctc tcttcgagta
      181 attacacaac tacagagaat aacgagagtt attaaatgta ggaagtggaa ttaagaggta
      241 gagagttaca cataagcaat cagtatgatt ggttaggtga ttactcttct gcgcacagcg
      301 caatagtatt tcttcgtggg cgttaccgcc acgatagtta cacgaagaaa gtaacgcttt
      361 gctttcggtg tgagcggaac gggccagcca cgaagcccga aaggtagcgg agtaaggctg
      421 cttgccagag agtgaagcta acgcc
//
LOCUS       DAZKHF020000810          443 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2201S31241
            SAMN43267800-rid25106413.denovo.815, whole genome shotgun sequence.
ACCESSION   DAZKHF020000810 DAZKHF020000000
VERSION     DAZKHF020000810.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267800
            Sequence Read Archive: SRR30307263
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 443)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 443)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 443)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350581.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350581.2
            Genome Coverage       :: 151x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:21:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,652
            CDSs (total)                      :: 5,557
            Genes (coding)                    :: 5,320
            CDSs (with protein)               :: 5,320
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 237
            CDSs (without protein)            :: 237
            Pseudo Genes (ambiguous residues) :: 0 of 237
            Pseudo Genes (frameshifted)       :: 102 of 237
            Pseudo Genes (incomplete)         :: 130 of 237
            Pseudo Genes (internal stop)      :: 52 of 237
            Pseudo Genes (multiple problems)  :: 41 of 237
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..443
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267800-rid25106413.denovo.815"
                     /serotype="O 26:H11"
                     /isolate="2201S31241"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-01"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            <1..>443
                     /locus_tag="ACHOSC_RS27610"
                     /old_locus_tag="pgaptmp_005522"
     CDS             <1..>443
                     /locus_tag="ACHOSC_RS27610"
                     /old_locus_tag="pgaptmp_005522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase small subunit"
                     /protein_id="HGU4551217.1"
                     /translation="LTDRQLRRLQKLPGAPISNKRGQLDLDAWRDFYISYLRRSKNDV
                     PDGDSEDDYEEKLLIARWELTAEQAVTQQLKNEVSKGKLIDTGFCIFALSKLAMALSS
                     TLDSIPLSMQRQFPDLTPRHLDHLKTLIAKGANQCARAGDKLPDLL"
BASE COUNT          125 a           97 c          113 g          108 t
ORIGIN      
        1 ctgaccgatc gccagcttcg cagattgcag aaattgccgg gtgccccgat atcaaataag
       61 cgagggcaac tggatctgga tgcctggcgc gatttttaca tatcgtatct gaggagaagt
      121 aaaaacgatg tgcctgatgg cgatagcgaa gacgactatg aggaaaaatt gcttattgcc
      181 agatgggaac tgacagcaga acaggctgtt acacagcagt taaaaaatga ggtgtcaaaa
      241 ggaaaactta ttgacaccgg gttctgtatt tttgccctca gtaagctggc aatggcgtta
      301 tccagtacgc ttgattccat ccctttatcc atgcaacgac agtttcctga tttaacaccg
      361 cgccatcttg accatctgaa aacccttatt gcgaaggggg caaatcagtg tgcgcgggcg
      421 ggggataaat taccggattt act
//
LOCUS       DAZKHF020000811          442 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2201S31241
            SAMN43267800-rid25106413.denovo.816, whole genome shotgun sequence.
ACCESSION   DAZKHF020000811 DAZKHF020000000
VERSION     DAZKHF020000811.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267800
            Sequence Read Archive: SRR30307263
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 442)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 442)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 442)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350581.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350581.2
            Genome Coverage       :: 151x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:21:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,652
            CDSs (total)                      :: 5,557
            Genes (coding)                    :: 5,320
            CDSs (with protein)               :: 5,320
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 237
            CDSs (without protein)            :: 237
            Pseudo Genes (ambiguous residues) :: 0 of 237
            Pseudo Genes (frameshifted)       :: 102 of 237
            Pseudo Genes (incomplete)         :: 130 of 237
            Pseudo Genes (internal stop)      :: 52 of 237
            Pseudo Genes (multiple problems)  :: 41 of 237
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..442
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267800-rid25106413.denovo.816"
                     /serotype="O 26:H11"
                     /isolate="2201S31241"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-01"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            <1..>442
                     /locus_tag="ACHOSC_RS27615"
                     /old_locus_tag="pgaptmp_005523"
     CDS             <1..>442
                     /locus_tag="ACHOSC_RS27615"
                     /old_locus_tag="pgaptmp_005523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="RHS repeat protein"
                     /protein_id="HGU4551218.1"
                     /translation="YDEADRLTHRTVKGETAERWQYDERGWLTDISHISEGHRVAVHY
                     GYDSKGRLASEHLTVHHPQTEALLWQHETRHAYNAQGLAKRCIPDSLPAVEWLTYGSG
                     YLAGMKLGDTPLVEYTRDRLHRETLRRFGRYELTTAYTPAGQLQR"
BASE COUNT          104 a          139 c          134 g           65 t
ORIGIN      
        1 actatgacga agcagaccgc ctcactcacc gcaccgtgaa gggtgaaacc gcagagcggt
       61 ggcagtatga cgaacgcggc tggctgacag acatcagcca tatcagcgaa gggcaccggg
      121 tggcggtgca ctatggttat gacagtaaag gccgcctcgc cagtgaacac ctgacggtgc
      181 atcatccgca gacggaagca ctgctctggc agcatgagac cagacatgcg tacaacgcgc
      241 agggactggc gaaacgctgt ataccggaca gcctgcccgc cgtggaatgg ctgacctacg
      301 gcagcggtta cctggcaggc atgaaactcg gcgacacacc gctggtggag tacacccgcg
      361 accgcctgca ccgggaaacg ctgcgccgct tcggccgtta tgaactcacc accgcttata
      421 cccctgccgg gcagttacag cg
//
LOCUS       DAZKHF020000812          439 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2201S31241
            SAMN43267800-rid25106413.denovo.817, whole genome shotgun sequence.
ACCESSION   DAZKHF020000812 DAZKHF020000000
VERSION     DAZKHF020000812.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267800
            Sequence Read Archive: SRR30307263
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 439)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 439)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 439)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350581.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350581.2
            Genome Coverage       :: 151x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:21:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,652
            CDSs (total)                      :: 5,557
            Genes (coding)                    :: 5,320
            CDSs (with protein)               :: 5,320
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 237
            CDSs (without protein)            :: 237
            Pseudo Genes (ambiguous residues) :: 0 of 237
            Pseudo Genes (frameshifted)       :: 102 of 237
            Pseudo Genes (incomplete)         :: 130 of 237
            Pseudo Genes (internal stop)      :: 52 of 237
            Pseudo Genes (multiple problems)  :: 41 of 237
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..439
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267800-rid25106413.denovo.817"
                     /serotype="O 26:H11"
                     /isolate="2201S31241"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-01"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            complement(84..380)
                     /locus_tag="ACHOSC_RS27620"
                     /old_locus_tag="pgaptmp_005524"
                     /pseudo
     CDS             complement(84..380)
                     /locus_tag="ACHOSC_RS27620"
                     /old_locus_tag="pgaptmp_005524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_047234906.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
BASE COUNT          101 a          116 c           89 g          133 t
ORIGIN      
        1 actttggttc ccactactta tttgttggac accactttgt ctaattcgtc agattctgac
       61 cagacggttc aggctgtacg cttacgatta cgctgaactt cacgtgagat cgtcgaagga
      121 ctgcgattca gcgcagtagc tatcgcacga atgctcattt tggctgacaa accagctcgt
      181 atctcctcgc gctcagacag tgtcaggtga gctacagccc gcttacgctc atggggtttt
      241 atgccgccag tatcccttaa catagtgaag atcgttccgg gttttgaacc caggatattc
      301 gctatttcac tgaagcctgt tccgttcttc catagttcaa aaacagaggc tttttcctct
      361 gctgtaaatg ttcgtctcat tcaaaaaacc tccgcaaccc catgttttca cataactgtt
      421 gcgttgacca attgaatct
//
LOCUS       DAZKHF020000813          438 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2201S31241
            SAMN43267800-rid25106413.denovo.818, whole genome shotgun sequence.
ACCESSION   DAZKHF020000813 DAZKHF020000000
VERSION     DAZKHF020000813.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267800
            Sequence Read Archive: SRR30307263
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 438)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 438)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 438)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350581.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350581.2
            Genome Coverage       :: 151x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:21:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,652
            CDSs (total)                      :: 5,557
            Genes (coding)                    :: 5,320
            CDSs (with protein)               :: 5,320
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 237
            CDSs (without protein)            :: 237
            Pseudo Genes (ambiguous residues) :: 0 of 237
            Pseudo Genes (frameshifted)       :: 102 of 237
            Pseudo Genes (incomplete)         :: 130 of 237
            Pseudo Genes (internal stop)      :: 52 of 237
            Pseudo Genes (multiple problems)  :: 41 of 237
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..438
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267800-rid25106413.denovo.818"
                     /serotype="O 26:H11"
                     /isolate="2201S31241"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-01"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            <1..>438
                     /locus_tag="ACHOSC_RS27625"
                     /old_locus_tag="pgaptmp_005525"
     CDS             <1..>438
                     /locus_tag="ACHOSC_RS27625"
                     /old_locus_tag="pgaptmp_005525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="exonuclease"
                     /protein_id="HGU4551219.1"
                     /translation="DKLSLEVQNAVLVKFGTCENITVDMAIDAQELLQEDVATFDGHI
                     VEALMKTPEINAMYPERKLFAIGWVKHKCNPGAKWPEIQAELRNWKKRQDAERKETGK
                     YTSVVDLASARVNQQNTENSAGKINPVTAAICREYKQTWKTLD"
BASE COUNT          141 a           99 c          109 g           89 t
ORIGIN      
        1 tgacaagctg tcccttgaag tacagaacgc ggtcctggtt aagttcggta catgtgaaaa
       61 catcaccgtt gatatggcga ttgacgcgca ggaattactg caggaagacg ttgctacctt
      121 tgacgggcat atcgttgaag cactgatgaa aacgcctgaa attaacgcta tgtatccgga
      181 acgcaaactg ttcgctatcg gatgggttaa acacaaatgt aatccgggtg ccaaatggcc
      241 cgaaattcag gctgaattac gtaactggaa aaaacggcag gacgcagagc gcaaagagac
      301 tggaaaatac acgtctgttg ttgatctcgc cagcgccaga gtcaatcaac agaacactga
      361 aaactcagca ggaaaaatca acccagtcac tgccgccatt tgtcgcgaat acaagcagac
      421 atggaaaacg ctggatga
//
LOCUS       DAZKHF020000814          436 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2201S31241
            SAMN43267800-rid25106413.denovo.819, whole genome shotgun sequence.
ACCESSION   DAZKHF020000814 DAZKHF020000000
VERSION     DAZKHF020000814.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267800
            Sequence Read Archive: SRR30307263
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 436)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 436)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 436)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350581.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350581.2
            Genome Coverage       :: 151x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:21:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,652
            CDSs (total)                      :: 5,557
            Genes (coding)                    :: 5,320
            CDSs (with protein)               :: 5,320
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 237
            CDSs (without protein)            :: 237
            Pseudo Genes (ambiguous residues) :: 0 of 237
            Pseudo Genes (frameshifted)       :: 102 of 237
            Pseudo Genes (incomplete)         :: 130 of 237
            Pseudo Genes (internal stop)      :: 52 of 237
            Pseudo Genes (multiple problems)  :: 41 of 237
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..436
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267800-rid25106413.denovo.819"
                     /serotype="O 26:H11"
                     /isolate="2201S31241"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-01"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            <1..>436
                     /gene="nleB"
                     /locus_tag="ACHOSC_RS27630"
                     /old_locus_tag="pgaptmp_005526"
     CDS             <1..>436
                     /gene="nleB"
                     /locus_tag="ACHOSC_RS27630"
                     /old_locus_tag="pgaptmp_005526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="type III secretion system effector arginine
                     glycosyltransferase NleB"
                     /protein_id="HGU4551220.1"
                     /translation="DNYLLGLLREELKNIPEGKDSLIESYAEKREHTWFDFFRNLAML
                     KAGTLFTETGKTGCHNISPCSGCIYLDADMIITDKLGVLYAPDGIAVHVDCNDEIKSL
                     ENGAIVVNRSNHPALLAGLDIMKNKVDAHPYYDGLGKGIKRHF"
BASE COUNT          138 a           63 c          102 g          133 t
ORIGIN      
        1 ggataattat ctgttaggct tattaagaga agagttaaaa aatatcccag aaggtaagga
       61 ctctttgatt gagtcatatg cagaaaaaag agaacatact tggtttgatt ttttcaggaa
      121 tttggccatg ttgaaggctg gaactttgtt tacagagacg ggaaaaactg gatgccataa
      181 catatcgccc tgtagcggat gtatatatct tgatgccgac atgattatta ccgataaatt
      241 aggagtcctg tatgctcctg atggtatcgc tgtgcatgta gattgtaatg atgagataaa
      301 aagtcttgaa aatggtgcga tagttgtcaa tcgtagtaat catccagcat tacttgcagg
      361 cctcgatatt atgaagaata aagttgacgc tcatccatat tatgatggtc taggaaaggg
      421 tatcaagcgg catttt
//
LOCUS       DAZKHF020000815          435 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2201S31241
            SAMN43267800-rid25106413.denovo.820, whole genome shotgun sequence.
ACCESSION   DAZKHF020000815 DAZKHF020000000
VERSION     DAZKHF020000815.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267800
            Sequence Read Archive: SRR30307263
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 435)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 435)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 435)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350581.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350581.2
            Genome Coverage       :: 151x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:21:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,652
            CDSs (total)                      :: 5,557
            Genes (coding)                    :: 5,320
            CDSs (with protein)               :: 5,320
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 237
            CDSs (without protein)            :: 237
            Pseudo Genes (ambiguous residues) :: 0 of 237
            Pseudo Genes (frameshifted)       :: 102 of 237
            Pseudo Genes (incomplete)         :: 130 of 237
            Pseudo Genes (internal stop)      :: 52 of 237
            Pseudo Genes (multiple problems)  :: 41 of 237
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..435
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267800-rid25106413.denovo.820"
                     /serotype="O 26:H11"
                     /isolate="2201S31241"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-01"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            <1..>435
                     /locus_tag="ACHOSC_RS27635"
                     /old_locus_tag="pgaptmp_005527"
     CDS             <1..>435
                     /locus_tag="ACHOSC_RS27635"
                     /old_locus_tag="pgaptmp_005527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HGU4551221.1"
                     /translation="RVARCTVARLMAVMGLAGVLRGKKVRTTISRKAVAAGDRVNRQF
                     VAERPDQLWVADFTYVSTWQGFVYVAFIIDVFAGYIVGWRVSSSMETTFVLDALEQAL
                     WARRPSGTVHHSDKGSQYVSLAYTQRLKEAGLLASTGSTGDS"
BASE COUNT           90 a          109 c          136 g          100 t
ORIGIN      
        1 tcagagtggc cagatgcact gtggcacgtc tcatggcagt tatgggactt gccggtgttc
       61 tccggggtaa aaaggtccgt acgaccatca gccggaaagc cgttgccgca ggcgaccgcg
      121 taaaccgtca gttcgtggca gaacgacctg accagctgtg ggtggctgat tttacttacg
      181 tcagcacatg gcagggcttc gtctatgtgg cgttcatcat tgatgtgttt gccggataca
      241 ttgtggggtg gcgggtctca tcgtccatgg agacgacatt cgtgctggat gcactggagc
      301 aggcgttatg ggcccgtcga ccgtccggca cggtccatca cagtgataaa ggttctcagt
      361 atgtatcgct ggcctacaca cagcggctta aggaagccgg attactggca tcaacaggaa
      421 gtacaggcga ctcgt
//
LOCUS       DAZKHF020000816          434 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2201S31241
            SAMN43267800-rid25106413.denovo.821, whole genome shotgun sequence.
ACCESSION   DAZKHF020000816 DAZKHF020000000
VERSION     DAZKHF020000816.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267800
            Sequence Read Archive: SRR30307263
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 434)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 434)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 434)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350581.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350581.2
            Genome Coverage       :: 151x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:21:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,652
            CDSs (total)                      :: 5,557
            Genes (coding)                    :: 5,320
            CDSs (with protein)               :: 5,320
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 237
            CDSs (without protein)            :: 237
            Pseudo Genes (ambiguous residues) :: 0 of 237
            Pseudo Genes (frameshifted)       :: 102 of 237
            Pseudo Genes (incomplete)         :: 130 of 237
            Pseudo Genes (internal stop)      :: 52 of 237
            Pseudo Genes (multiple problems)  :: 41 of 237
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..434
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267800-rid25106413.denovo.821"
                     /serotype="O 26:H11"
                     /isolate="2201S31241"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-01"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            complement(<1..407)
                     /locus_tag="ACHOSC_RS27640"
                     /old_locus_tag="pgaptmp_005528"
     CDS             complement(<1..407)
                     /locus_tag="ACHOSC_RS27640"
                     /old_locus_tag="pgaptmp_005528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ZinT/AdcA family metal-binding protein"
                     /protein_id="HGU4551222.1"
                     /translation="MAIRLHKLAVALGVFIVSAPAFSHGHHSHGKPLTEVEQKAANGV
                     FDDANVQNRTLSDWDGVWQSVYPLLQSGKLDPIFQKKADADKTKTFAEIKDYYRKGYV
                     TDIEMIGIEDGIVEFHRNNETTSCKYDYDGYKIL"
BASE COUNT          123 a           91 c           84 g          136 t
ORIGIN      
        1 agtattttgt atccatcgta atcatattta caggatgttg tttcattatt tctatggaat
       61 tcgacaatgc cgtcctcaat gccaatcatc tcgatgtctg tcacataacc tttgcgataa
      121 taatctttaa tttcagcaaa tgttttggtt ttatctgcat ccgctttctt ctgaaagatg
      181 gggtcaagtt tgccactttg cagtaaagga taaacggatt gccagactcc atcccagtca
      241 ctgagcgttc ggttttgtac attggcatca tcaaaaacac cattagcagc tttttgttcg
      301 acctctgtta agggtttgcc gtgtgagtga tgaccatgcg aaaaggcagg agcgctaaca
      361 ataaagacac ctaaagcaac agccagttta tgaagacgaa tcgccaaaac agtttcctcc
      421 agagttaatg tata
//
LOCUS       DAZKHF020000817          432 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2201S31241
            SAMN43267800-rid25106413.denovo.822, whole genome shotgun sequence.
ACCESSION   DAZKHF020000817 DAZKHF020000000
VERSION     DAZKHF020000817.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267800
            Sequence Read Archive: SRR30307263
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 432)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 432)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 432)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350581.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350581.2
            Genome Coverage       :: 151x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:21:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,652
            CDSs (total)                      :: 5,557
            Genes (coding)                    :: 5,320
            CDSs (with protein)               :: 5,320
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 237
            CDSs (without protein)            :: 237
            Pseudo Genes (ambiguous residues) :: 0 of 237
            Pseudo Genes (frameshifted)       :: 102 of 237
            Pseudo Genes (incomplete)         :: 130 of 237
            Pseudo Genes (internal stop)      :: 52 of 237
            Pseudo Genes (multiple problems)  :: 41 of 237
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..432
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267800-rid25106413.denovo.822"
                     /serotype="O 26:H11"
                     /isolate="2201S31241"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-01"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            <1..>432
                     /locus_tag="ACHOSC_RS27645"
                     /old_locus_tag="pgaptmp_005529"
     CDS             <1..>432
                     /locus_tag="ACHOSC_RS27645"
                     /old_locus_tag="pgaptmp_005529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SdiA-regulated domain-containing protein"
                     /protein_id="HGU4551223.1"
                     /translation="KQLPNTVNLSSLTYNKEDDFLYATQNSPAQLLKIARSGDIIGRA
                     PLPFISDAETIEHIQGNIFAAVDEKTSELFFFTVTKDMHISLRNKIQLEKFNKKNRGF
                     EGLAWKAEGRMLFVAKERRPTGMFAYQLSPDLLSAKQVTIPE"
BASE COUNT          143 a           93 c           87 g          109 t
ORIGIN      
        1 aaacaattac caaatacggt gaacttatca tctcttacgt ataataaaga ggatgatttc
       61 ctgtatgcca cccagaatag ccctgcgcag ctacttaaaa tagccagaag tggcgacatc
      121 atcggccgag ccccgctgcc ttttatctct gatgccgaaa ccattgagca catacaaggg
      181 aacatttttg ctgcagtaga cgaaaagact tctgaactat tcttttttac agttacaaaa
      241 gatatgcata tctccttgcg aaataaaatt cagttagaaa aattcaataa aaaaaataga
      301 gggtttgaag gactggcatg gaaagcagaa ggccggatgc tgtttgtcgc taaagaacga
      361 cgccctacag gcatgtttgc ctatcagctt tctccagatc tgctgagtgc aaaacaagta
      421 acgattccag ag
//
LOCUS       DAZKHF020000818          431 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2201S31241
            SAMN43267800-rid25106413.denovo.823, whole genome shotgun sequence.
ACCESSION   DAZKHF020000818 DAZKHF020000000
VERSION     DAZKHF020000818.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267800
            Sequence Read Archive: SRR30307263
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 431)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 431)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 431)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350581.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350581.2
            Genome Coverage       :: 151x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:21:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,652
            CDSs (total)                      :: 5,557
            Genes (coding)                    :: 5,320
            CDSs (with protein)               :: 5,320
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 237
            CDSs (without protein)            :: 237
            Pseudo Genes (ambiguous residues) :: 0 of 237
            Pseudo Genes (frameshifted)       :: 102 of 237
            Pseudo Genes (incomplete)         :: 130 of 237
            Pseudo Genes (internal stop)      :: 52 of 237
            Pseudo Genes (multiple problems)  :: 41 of 237
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..431
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267800-rid25106413.denovo.823"
                     /serotype="O 26:H11"
                     /isolate="2201S31241"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-01"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            complement(<1..>431)
                     /locus_tag="ACHOSC_RS27650"
                     /old_locus_tag="pgaptmp_005530"
     CDS             complement(<1..>431)
                     /locus_tag="ACHOSC_RS27650"
                     /old_locus_tag="pgaptmp_005530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0019082 - viral protein processing
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="HK97 family phage prohead protease"
                     /protein_id="HGU4551224.1"
                     /translation="TLVKPVPDMPSQLAARLDEVWAAIKTGLVRGLSVGFRPHEYTFL
                     DGGGLHFLRWELMEVSAVTVPANAECTIRTIKSYDRPFSAASGNRKPVVKIASSAGAA
                     AQSTTVFHKEKTIMNIGEQIKSFENKRAALAASLEEVMTKA"
BASE COUNT           89 a          125 c          120 g           97 t
ORIGIN      
        1 ggctttggtc atgacctcct caagggaggc tgccagcgct gcacgcttgt tttcaaaact
       61 tttaatctgt tcgccaatat tcattatggt cttttcctta tgaaaaacgg ttgttgactg
      121 tgccgcagcg ccggcagaag atgcgatttt caccaccggt ttccggttgc cggacgcggc
      181 agaaaacggg cggtcgtaag atttaatggt ccggatggtg cattccgcat tcgcgggcac
      241 ggtgacggca gacacctcca tcagttccca gcgcagaaaa tgcagtccgc ctccgtccag
      301 aaaggtgtat tcatggggac ggaagcccac ggacagcccc ctgaccagcc cggtcttaat
      361 ggccgcccag acctcatcca gccgggcagc cagttgcgac ggcatatccg gtacgggctt
      421 caccagtgtt g
//
LOCUS       DAZKHF020000819          430 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2201S31241
            SAMN43267800-rid25106413.denovo.824, whole genome shotgun sequence.
ACCESSION   DAZKHF020000819 DAZKHF020000000
VERSION     DAZKHF020000819.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267800
            Sequence Read Archive: SRR30307263
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 430)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 430)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 430)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350581.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350581.2
            Genome Coverage       :: 151x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:21:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,652
            CDSs (total)                      :: 5,557
            Genes (coding)                    :: 5,320
            CDSs (with protein)               :: 5,320
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 237
            CDSs (without protein)            :: 237
            Pseudo Genes (ambiguous residues) :: 0 of 237
            Pseudo Genes (frameshifted)       :: 102 of 237
            Pseudo Genes (incomplete)         :: 130 of 237
            Pseudo Genes (internal stop)      :: 52 of 237
            Pseudo Genes (multiple problems)  :: 41 of 237
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..430
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267800-rid25106413.denovo.824"
                     /serotype="O 26:H11"
                     /isolate="2201S31241"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-01"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            <1..>430
                     /locus_tag="ACHOSC_RS27655"
                     /old_locus_tag="pgaptmp_005531"
     CDS             <1..>430
                     /locus_tag="ACHOSC_RS27655"
                     /old_locus_tag="pgaptmp_005531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001429641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pentapeptide repeat-containing protein"
                     /protein_id="HGU4551225.1"
                     /translation="GRACLSKAELTADLMWLSANRTGEESAEQLNYSGCDLSGLSLAG
                     LNFASVNFSDSRFDDADLSGCNLSGASLKNTSFKNSVLEECDLSFSDLTNSTISASFD
                     RANFSGANLNSASFIGSSFKETPPDLKDAQLAGAIIVPGME"
BASE COUNT          109 a           83 c          104 g          134 t
ORIGIN      
        1 ggccgtgcct gcctgagcaa agctgagctt actgctgact taatgtggtt gtcagctaac
       61 cgaacggggg aagagtctgc tgaacaatta aattactctg gatgtgactt gtcaggtcta
      121 agtcttgcag ggctgaattt tgcatcagta aatttttctg attccagatt tgatgatgct
      181 gatcttagtg gctgtaacct gtcaggtgca tccctgaaaa acacatcttt caagaattct
      241 gttctggagg agtgtgattt atctttctct gatttgacaa atagcacaat cagcgcatcg
      301 tttgatagag ctaattttag tggtgctaat cttaacagcg catcgtttat tggttcttca
      361 tttaaagaaa cacctccaga cctgaaagat gcacagttgg cgggggctat tatagttccc
      421 gggatggagc
//
LOCUS       DAZKHF020000820          428 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2201S31241
            SAMN43267800-rid25106413.denovo.825, whole genome shotgun sequence.
ACCESSION   DAZKHF020000820 DAZKHF020000000
VERSION     DAZKHF020000820.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267800
            Sequence Read Archive: SRR30307263
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 428)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 428)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 428)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350581.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350581.2
            Genome Coverage       :: 151x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:21:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,652
            CDSs (total)                      :: 5,557
            Genes (coding)                    :: 5,320
            CDSs (with protein)               :: 5,320
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 237
            CDSs (without protein)            :: 237
            Pseudo Genes (ambiguous residues) :: 0 of 237
            Pseudo Genes (frameshifted)       :: 102 of 237
            Pseudo Genes (incomplete)         :: 130 of 237
            Pseudo Genes (internal stop)      :: 52 of 237
            Pseudo Genes (multiple problems)  :: 41 of 237
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..428
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267800-rid25106413.denovo.825"
                     /serotype="O 26:H11"
                     /isolate="2201S31241"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-01"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            complement(47..112)
                     /locus_tag="ACHOSC_RS27660"
                     /old_locus_tag="pgaptmp_005532"
                     /pseudo
     CDS             complement(47..112)
                     /locus_tag="ACHOSC_RS27660"
                     /old_locus_tag="pgaptmp_005532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310292.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tellurite resistance TerB family protein"
BASE COUNT          127 a          104 c           81 g          116 t
ORIGIN      
        1 acagcgcagg ctgattacgc agtacctgtt cgatcttcgc tttctaggaa gcctcacatt
       61 caccatctgc acaggccacc aggtaggcag cattaataac cgcctgtgcc agatcgcgtt
      121 tctcaaactt tcctttttcc ggttaacatg acataccaat aactcttgtc taaaaagcca
      181 gcaagataaa aagccagtat tcacgaccac cagcgtgttt actgtactgc acaaggttta
      241 caggtacaaa aaaaccgctc agcggcgggt ttaagttgtg tggcgaagta accactctta
      301 acacgatata atactttttg cgtacgcgtt aggattttta taaactatgc gtccccattc
      361 tcgctatctt tagtcggtcc tggaatacac atgaaagtta gaagcatagg atttacaata
      421 aataacaa
//
LOCUS       DAZKHF020000821          424 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2201S31241
            SAMN43267800-rid25106413.denovo.826, whole genome shotgun sequence.
ACCESSION   DAZKHF020000821 DAZKHF020000000
VERSION     DAZKHF020000821.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267800
            Sequence Read Archive: SRR30307263
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 424)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 424)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 424)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350581.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350581.2
            Genome Coverage       :: 151x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:21:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,652
            CDSs (total)                      :: 5,557
            Genes (coding)                    :: 5,320
            CDSs (with protein)               :: 5,320
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 237
            CDSs (without protein)            :: 237
            Pseudo Genes (ambiguous residues) :: 0 of 237
            Pseudo Genes (frameshifted)       :: 102 of 237
            Pseudo Genes (incomplete)         :: 130 of 237
            Pseudo Genes (internal stop)      :: 52 of 237
            Pseudo Genes (multiple problems)  :: 41 of 237
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..424
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267800-rid25106413.denovo.826"
                     /serotype="O 26:H11"
                     /isolate="2201S31241"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-01"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            <1..330
                     /locus_tag="ACHOSC_RS27665"
                     /old_locus_tag="pgaptmp_005533"
     CDS             <1..330
                     /locus_tag="ACHOSC_RS27665"
                     /old_locus_tag="pgaptmp_005533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="major tail subunit"
                     /protein_id="HGU4551226.1"
                     /translation="ATFSLRMKGKPVSFVVPLAFVKNLDKTLTVNTGALLTMSVSANG
                     GTPPYKYAWKKDGQPVDGQTTDTFSKPGAQSADAGKYTCVVTDSAEKAQSVTSVECTV
                     TVSAAAG"
     gene            297..>424
                     /locus_tag="ACHOSC_RS27670"
                     /old_locus_tag="pgaptmp_005534"
     CDS             297..>424
                     /locus_tag="ACHOSC_RS27670"
                     /old_locus_tag="pgaptmp_005534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013009258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail assembly chaperone"
                     /protein_id="HGU4551227.1"
                     /translation="MHRDSERSRRIRGWVIMKKDLKTLALARLSGFRHKTVKVPEWG"
BASE COUNT          111 a           93 c          137 g           83 t
ORIGIN      
        1 gcaacgtttt cactgcgtat gaaaggcaaa ccggtgtcct ttgtggtacc gctggcgttt
       61 gtgaaaaatc tggataaaac acttaccgtg aatacaggtg cgctgctgac aatgtcagtc
      121 agtgccaacg ggggaacgcc gccgtataaa tacgcctgga agaaggatgg tcagccggtt
      181 gacgggcaga cgacagacac cttcagtaag ccaggtgcgc agtccgctga tgcggggaaa
      241 tatacctgcg tggtgaccga ttcggcagag aaagcacaga gtgtgacgtc tgttgaatgc
      301 accgtgacag tgagcgcagc cgccggataa ggggatgggt catcatgaaa aaggatctga
      361 aaacgctggc gctggccaga ctgtcagggt ttcgtcataa aacggtgaag gtgccggaat
      421 gggg
//
LOCUS       DAZKHF020000822          420 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2201S31241
            SAMN43267800-rid25106413.denovo.827, whole genome shotgun sequence.
ACCESSION   DAZKHF020000822 DAZKHF020000000
VERSION     DAZKHF020000822.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267800
            Sequence Read Archive: SRR30307263
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 420)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 420)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 420)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350581.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350581.2
            Genome Coverage       :: 151x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:21:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,652
            CDSs (total)                      :: 5,557
            Genes (coding)                    :: 5,320
            CDSs (with protein)               :: 5,320
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 237
            CDSs (without protein)            :: 237
            Pseudo Genes (ambiguous residues) :: 0 of 237
            Pseudo Genes (frameshifted)       :: 102 of 237
            Pseudo Genes (incomplete)         :: 130 of 237
            Pseudo Genes (internal stop)      :: 52 of 237
            Pseudo Genes (multiple problems)  :: 41 of 237
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..420
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267800-rid25106413.denovo.827"
                     /serotype="O 26:H11"
                     /isolate="2201S31241"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-01"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            complement(<1..>420)
                     /locus_tag="ACHOSC_RS27675"
                     /old_locus_tag="pgaptmp_005535"
     CDS             complement(<1..>420)
                     /locus_tag="ACHOSC_RS27675"
                     /old_locus_tag="pgaptmp_005535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HGU4551228.1"
                     /translation="SELDTQSAMDFILGANSQEQRERLTGWIGEIAAYYSAAPVRLGG
                     AKVPHLMPGDSLNLQTAQDTDNGYSVFEQSLLRYIAAGLGVSYEQLSRNYAQMSYSTA
                     RASANESWAYFMGRRKFVASRQASQMFLCWLEEAIVRR"
BASE COUNT           86 a          140 c          111 g           83 t
ORIGIN      
        1 gcggcgaacg atggcctctt ccagccagca cagaaacatc tggctcgcct gacgggatgc
       61 gacgaatttt cgccgcccca taaagtacgc ccacgactcg ttcgcactgg cccgtgccgt
      121 ggagtagctc atctgggcgt aattccggga aagctgctca tacgagacac ccagcccggc
      181 agcgatatac cgcagcagtg actgctcaaa cacggagtag ccgttatccg tgtcctgagc
      241 cgtctgcagg ttcagtgagt cccccggcat caggtgcggc acttttgcgc ctcccagacg
      301 gaccggtgct gcggagtaat acgcggcaat ttcaccaatc cagccggtca gcctttcccg
      361 ctgctcctga ctgttcgcgc ccagaataaa atccatcgct gactgcgtat ccagctcact
//
LOCUS       DAZKHF020000823          416 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2201S31241
            SAMN43267800-rid25106413.denovo.828, whole genome shotgun sequence.
ACCESSION   DAZKHF020000823 DAZKHF020000000
VERSION     DAZKHF020000823.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267800
            Sequence Read Archive: SRR30307263
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 416)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 416)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 416)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350581.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350581.2
            Genome Coverage       :: 151x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:21:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,652
            CDSs (total)                      :: 5,557
            Genes (coding)                    :: 5,320
            CDSs (with protein)               :: 5,320
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 237
            CDSs (without protein)            :: 237
            Pseudo Genes (ambiguous residues) :: 0 of 237
            Pseudo Genes (frameshifted)       :: 102 of 237
            Pseudo Genes (incomplete)         :: 130 of 237
            Pseudo Genes (internal stop)      :: 52 of 237
            Pseudo Genes (multiple problems)  :: 41 of 237
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..416
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267800-rid25106413.denovo.828"
                     /serotype="O 26:H11"
                     /isolate="2201S31241"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-01"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            <1..>416
                     /locus_tag="ACHOSC_RS27680"
                     /old_locus_tag="pgaptmp_005536"
     CDS             <1..>416
                     /locus_tag="ACHOSC_RS27680"
                     /old_locus_tag="pgaptmp_005536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000104949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HGU4551229.1"
                     /translation="HPDAVVFSPKKRQWGTQDDLTCAQWLWKKIIALYEQAAECDGEV
                     VRPKEPNWTAWANEIRLMCVQDGRTHKQICEMYSRVSRDPFWCRNVLSPSKLREKWDE
                     LSLRLSPSVSTYTEKREDPYFKSSYDNVDYSQIPAG"
BASE COUNT          108 a          115 c          114 g           79 t
ORIGIN      
        1 ccatcctgat gcggttgtat tcagccctaa aaagcgccag tggggaacgc aggatgattt
       61 gacctgcgca cagtggctct ggaaaaaaat catcgccctg tacgagcaag ccgccgaatg
      121 tgacggcgag gtggttcgtc ccaaagaacc gaactggaca gcctgggcaa acgaaattcg
      181 cctgatgtgt gtgcaggatg gtcgtactca caaacaaatc tgcgagatgt acagccgcgt
      241 cagccgcgat ccgttctggt gccgtaacgt gctcagcccg tcgaagttgc gggaaaaatg
      301 ggatgagctt tccctgcgct tatcaccgtc cgtcagcacg tacaccgaaa aacgcgaaga
      361 cccgtacttc aaatccagtt acgacaacgt ggactacagc cagatcccgg caggat
//
LOCUS       DAZKHF020000824          413 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2201S31241
            SAMN43267800-rid25106413.denovo.829, whole genome shotgun sequence.
ACCESSION   DAZKHF020000824 DAZKHF020000000
VERSION     DAZKHF020000824.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267800
            Sequence Read Archive: SRR30307263
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 413)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 413)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 413)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350581.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350581.2
            Genome Coverage       :: 151x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:21:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,652
            CDSs (total)                      :: 5,557
            Genes (coding)                    :: 5,320
            CDSs (with protein)               :: 5,320
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 237
            CDSs (without protein)            :: 237
            Pseudo Genes (ambiguous residues) :: 0 of 237
            Pseudo Genes (frameshifted)       :: 102 of 237
            Pseudo Genes (incomplete)         :: 130 of 237
            Pseudo Genes (internal stop)      :: 52 of 237
            Pseudo Genes (multiple problems)  :: 41 of 237
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..413
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267800-rid25106413.denovo.829"
                     /serotype="O 26:H11"
                     /isolate="2201S31241"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-01"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            <1..>413
                     /locus_tag="ACHOSC_RS27685"
                     /old_locus_tag="pgaptmp_005537"
     CDS             <1..>413
                     /locus_tag="ACHOSC_RS27685"
                     /old_locus_tag="pgaptmp_005537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="RHS repeat protein"
                     /protein_id="HGU4551230.1"
                     /translation="REYDEPGRLVSETSRSGETVRYRYDDAHSELPATTTDATGSTRQ
                     MTWSRYGQLLAFTDCSGYQTRYEYDRFGQMTAVHREEGISLYRHYDNRGRLTSVKDAQ
                     GRETQYEYNAAGDLTAVITPDGNRSETQYDAWGKA"
BASE COUNT           98 a          119 c          137 g           59 t
ORIGIN      
        1 ccgggaatat gatgaaccgg gcaggctggt atcggagaca tcgcgcagcg gggagacagt
       61 acgctaccgc tacgatgacg cgcacagtga gttaccggcg acgacaacgg atgcgacggg
      121 cagcacccgg cagatgacgt ggagccgcta cgggcagttg ctggcgttca ctgactgctc
      181 gggctaccag acccgctatg aatacgaccg cttcggccag atgacggcgg ttcaccgcga
      241 ggaaggtatc agcctttacc gccactatga caaccgtggc cggttaacct cggtgaaaga
      301 cgcacagggc cgtgaaacgc agtatgagta caacgccgca ggcgacctga ctgccgttat
      361 caccccggac ggcaaccgga gcgagacaca gtacgatgcg tggggaaagg cgg
//
LOCUS       DAZKHF020000825          411 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2201S31241
            SAMN43267800-rid25106413.denovo.830, whole genome shotgun sequence.
ACCESSION   DAZKHF020000825 DAZKHF020000000
VERSION     DAZKHF020000825.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267800
            Sequence Read Archive: SRR30307263
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 411)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 411)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 411)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350581.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350581.2
            Genome Coverage       :: 151x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:21:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,652
            CDSs (total)                      :: 5,557
            Genes (coding)                    :: 5,320
            CDSs (with protein)               :: 5,320
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 237
            CDSs (without protein)            :: 237
            Pseudo Genes (ambiguous residues) :: 0 of 237
            Pseudo Genes (frameshifted)       :: 102 of 237
            Pseudo Genes (incomplete)         :: 130 of 237
            Pseudo Genes (internal stop)      :: 52 of 237
            Pseudo Genes (multiple problems)  :: 41 of 237
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..411
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267800-rid25106413.denovo.830"
                     /serotype="O 26:H11"
                     /isolate="2201S31241"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-01"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            204..>411
                     /locus_tag="ACHOSC_RS27690"
                     /old_locus_tag="pgaptmp_005538"
     CDS             204..>411
                     /locus_tag="ACHOSC_RS27690"
                     /old_locus_tag="pgaptmp_005538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000256572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="regulator"
                     /protein_id="HGU4551231.1"
                     /translation="MVTIVWKESKGTAKSRYKARRAELIAERRSNEALARKIALKLSG
                     CVRADKAASLGSLCCKKKEEVVRKN"
BASE COUNT          136 a           92 c          107 g           76 t
ORIGIN      
        1 ttaattttaa gccgtcagca ggaggctgga agccaaacgg aacagattgg caggctcttt
       61 aacatcgacg aactctcaac ctaaccgttg agaccagaac ttgagtggtt ttggggatgg
      121 cgcgaattgc agctgcaaga cagcgatcga gaagataagc acctcgacgc gtcatgcgcc
      181 aaagccactt aaaggagacc atcatggtaa ccattgtctg gaaagaatcc aaaggtacgg
      241 caaaaagccg ctacaaagct cgcagagcag aacttattgc cgagcgacgc agtaatgaag
      301 cactggcgcg aaaaattgcg ctaaagctct ctggttgcgt cagagcagac aaagcagcat
      361 cactaggaag cctttgctgc aagaagaaag aagaagttgt tcgaaaaaat a
//
LOCUS       DAZKHF020000826          409 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2201S31241
            SAMN43267800-rid25106413.denovo.831, whole genome shotgun sequence.
ACCESSION   DAZKHF020000826 DAZKHF020000000
VERSION     DAZKHF020000826.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267800
            Sequence Read Archive: SRR30307263
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 409)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 409)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 409)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350581.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350581.2
            Genome Coverage       :: 151x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:21:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,652
            CDSs (total)                      :: 5,557
            Genes (coding)                    :: 5,320
            CDSs (with protein)               :: 5,320
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 237
            CDSs (without protein)            :: 237
            Pseudo Genes (ambiguous residues) :: 0 of 237
            Pseudo Genes (frameshifted)       :: 102 of 237
            Pseudo Genes (incomplete)         :: 130 of 237
            Pseudo Genes (internal stop)      :: 52 of 237
            Pseudo Genes (multiple problems)  :: 41 of 237
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..409
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267800-rid25106413.denovo.831"
                     /serotype="O 26:H11"
                     /isolate="2201S31241"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-01"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            complement(<1..79)
                     /locus_tag="ACHOSC_RS27695"
                     /old_locus_tag="pgaptmp_005539"
     CDS             complement(<1..79)
                     /locus_tag="ACHOSC_RS27695"
                     /old_locus_tag="pgaptmp_005539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4551232.1"
                     /translation="MSHAVDGTYIKAQQDKMVNSSLCKKA"
     gene            97..288
                     /gene="yfgG"
                     /locus_tag="ACHOSC_RS27700"
                     /old_locus_tag="pgaptmp_005540"
     CDS             97..288
                     /gene="yfgG"
                     /locus_tag="ACHOSC_RS27700"
                     /old_locus_tag="pgaptmp_005540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfgG family protein"
                     /protein_id="HGU4551233.1"
                     /translation="MSQATSMRKRHRFNSRMTRIVLLISFIFFFGRFIYSSVGAWQHH
                     QSKKEAQQSTLSVESPVQR"
BASE COUNT          100 a          108 c           77 g          124 t
ORIGIN      
        1 acgctttttt acataacgaa gaattgacca ttttgtcctg ttgtgcctta atgtaagtac
       61 cgtccacagc gtgggacata cttcaaggaa ccttttgtga gtcaggcaac cagtatgcga
      121 aaacgacacc gatttaacag tcgcatgacc cgtatcgtac tgctcatcag ctttatcttc
      181 ttctttggcc gttttatcta ctcgtccgtc ggtgcctggc agcaccatca gagcaaaaaa
      241 gaagctcagc aatccacact ctccgtcgaa tcaccggtac aacgttagcg gttaccttct
      301 ccactttcac agaacctaac ggcacttcgc tgtcggatgc ttttgctctt tggaattatc
      361 aaagcggcag atattctttc atcttaaatt ttacgtcttt gtcctgact
//
LOCUS       DAZKHF020000827          409 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2201S31241
            SAMN43267800-rid25106413.denovo.832, whole genome shotgun sequence.
ACCESSION   DAZKHF020000827 DAZKHF020000000
VERSION     DAZKHF020000827.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267800
            Sequence Read Archive: SRR30307263
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 409)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 409)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 409)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350581.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350581.2
            Genome Coverage       :: 151x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:21:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,652
            CDSs (total)                      :: 5,557
            Genes (coding)                    :: 5,320
            CDSs (with protein)               :: 5,320
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 237
            CDSs (without protein)            :: 237
            Pseudo Genes (ambiguous residues) :: 0 of 237
            Pseudo Genes (frameshifted)       :: 102 of 237
            Pseudo Genes (incomplete)         :: 130 of 237
            Pseudo Genes (internal stop)      :: 52 of 237
            Pseudo Genes (multiple problems)  :: 41 of 237
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..409
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267800-rid25106413.denovo.832"
                     /serotype="O 26:H11"
                     /isolate="2201S31241"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-01"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            12..>409
                     /locus_tag="ACHOSC_RS27705"
                     /old_locus_tag="pgaptmp_005541"
     CDS             12..>409
                     /locus_tag="ACHOSC_RS27705"
                     /old_locus_tag="pgaptmp_005541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001038620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HEPN domain-containing protein"
                     /protein_id="HGU4551234.1"
                     /translation="MNKNDLEALSDTRLNEAKCLLDHGFFHGAYYLCGYAVECALKAC
                     IAKSFLQHEFPNKKVVNDSYTHDLSQLLKIANLHQILIADAKNDVSLEINWSVVKDWS
                     EQFRYDNNISKAMAEQLFDAVGDQNSGVLK"
BASE COUNT          129 a           64 c           84 g          132 t
ORIGIN      
        1 gttataattg tatgaataaa aacgaccttg aagcattatc tgacactagg cttaatgagg
       61 ccaaatgctt gcttgatcat ggcttttttc atggtgcata ttatctttgt gggtatgcag
      121 ttgaatgtgc attgaaggcc tgtattgcca agtcattttt acaacatgag tttccaaaca
      181 aaaaagtcgt aaatgattca tatactcatg atttgtcgca acttctcaaa attgccaact
      241 tacatcaaat tttgattgct gacgcaaaaa atgatgtttc gttggagatt aactggtcgg
      301 tcgttaaaga ctggagcgag caatttagat acgacaataa cataagtaaa gctatggccg
      361 aacaattgtt tgatgctgta ggtgaccaaa attctggagt tttgaaatg
//
LOCUS       DAZKHF020000828          407 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2201S31241
            SAMN43267800-rid25106413.denovo.833, whole genome shotgun sequence.
ACCESSION   DAZKHF020000828 DAZKHF020000000
VERSION     DAZKHF020000828.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267800
            Sequence Read Archive: SRR30307263
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 407)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 407)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 407)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350581.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350581.2
            Genome Coverage       :: 151x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:21:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,652
            CDSs (total)                      :: 5,557
            Genes (coding)                    :: 5,320
            CDSs (with protein)               :: 5,320
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 237
            CDSs (without protein)            :: 237
            Pseudo Genes (ambiguous residues) :: 0 of 237
            Pseudo Genes (frameshifted)       :: 102 of 237
            Pseudo Genes (incomplete)         :: 130 of 237
            Pseudo Genes (internal stop)      :: 52 of 237
            Pseudo Genes (multiple problems)  :: 41 of 237
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..407
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267800-rid25106413.denovo.833"
                     /serotype="O 26:H11"
                     /isolate="2201S31241"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-01"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            complement(1..339)
                     /locus_tag="ACHOSC_RS27710"
                     /old_locus_tag="pgaptmp_005542"
                     /pseudo
     CDS             complement(1..339)
                     /locus_tag="ACHOSC_RS27710"
                     /old_locus_tag="pgaptmp_005542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001347735.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
BASE COUNT           96 a          104 c           99 g          108 t
ORIGIN      
        1 acctgattct ggaaaaggac tttgtggatt gttgctattg ccagtgagat tgaatgaagt
       61 cgttttaact cgctttgaat acgtcttgcc cccagatttc gctgagaacg taactccaga
      121 atcagagcga cttcgcaagt gccggttttc gttgatggtg aacgcttggg gcgacggctg
      181 tggctttcca gaccagcgat gccctgagcc agatatctgc gccaccattt tacgcaatgt
      241 gggccgagaa atgccacagc gacggcatac aaatcccgca tcaccggacg tttcatagag
      301 ttttacccac tgtagccgct gctgaatttc cctgttcata atttggcatt agttcagctc
      361 aatcccgact taaaaagtcc ctacaggatg ttccggacct gaggcac
//
LOCUS       DAZKHF020000829          407 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2201S31241
            SAMN43267800-rid25106413.denovo.834, whole genome shotgun sequence.
ACCESSION   DAZKHF020000829 DAZKHF020000000
VERSION     DAZKHF020000829.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267800
            Sequence Read Archive: SRR30307263
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 407)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 407)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 407)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350581.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350581.2
            Genome Coverage       :: 151x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:21:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,652
            CDSs (total)                      :: 5,557
            Genes (coding)                    :: 5,320
            CDSs (with protein)               :: 5,320
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 237
            CDSs (without protein)            :: 237
            Pseudo Genes (ambiguous residues) :: 0 of 237
            Pseudo Genes (frameshifted)       :: 102 of 237
            Pseudo Genes (incomplete)         :: 130 of 237
            Pseudo Genes (internal stop)      :: 52 of 237
            Pseudo Genes (multiple problems)  :: 41 of 237
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..407
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267800-rid25106413.denovo.834"
                     /serotype="O 26:H11"
                     /isolate="2201S31241"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-01"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            complement(<1..211)
                     /locus_tag="ACHOSC_RS27715"
                     /old_locus_tag="pgaptmp_005543"
     CDS             complement(<1..211)
                     /locus_tag="ACHOSC_RS27715"
                     /old_locus_tag="pgaptmp_005543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system tip protein VgrG"
                     /protein_id="HGU4551235.1"
                     /translation="MSTGLRFTLEVDGLPPDAFAVVSFHLNQSLSSLFSLDLSLVSQQ
                     FLSLEFAQVLDKMAYLTIWQGDELQR"
BASE COUNT          126 a           84 c          104 g           93 t
ORIGIN      
        1 ggcgctgtaa ttcatcgccc tgccatatcg tcaggtaggc cattttgtcc agcacctgcg
       61 caaattcaag ggagagaaac tgctggctga ccagggagag atcgagggaa aagagcgaag
      121 agagtgactg gttcagatga aaggaaacca ccgcaaaagc gtccggcggc aggccgtcca
      181 cttccagtgt gaaacgtaat ccggttgaca tagcaacctc cggtaaaatg aataatgatc
      241 cgtaccgtac aactgcggat aatgtgataa gcgggacgac ttcagacgtt attagggaag
      301 gaacagaaaa tgctatggtt atgtgagaat tttgtttaaa tattacttta cagaaacggg
      361 tgtctgttca gtcaaaaaaa taatcacaag ctcaacctct tgtttct
//
LOCUS       DAZKHF020000830          407 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2201S31241
            SAMN43267800-rid25106413.denovo.835, whole genome shotgun sequence.
ACCESSION   DAZKHF020000830 DAZKHF020000000
VERSION     DAZKHF020000830.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267800
            Sequence Read Archive: SRR30307263
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 407)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 407)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 407)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350581.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350581.2
            Genome Coverage       :: 151x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:21:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,652
            CDSs (total)                      :: 5,557
            Genes (coding)                    :: 5,320
            CDSs (with protein)               :: 5,320
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 237
            CDSs (without protein)            :: 237
            Pseudo Genes (ambiguous residues) :: 0 of 237
            Pseudo Genes (frameshifted)       :: 102 of 237
            Pseudo Genes (incomplete)         :: 130 of 237
            Pseudo Genes (internal stop)      :: 52 of 237
            Pseudo Genes (multiple problems)  :: 41 of 237
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..407
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267800-rid25106413.denovo.835"
                     /serotype="O 26:H11"
                     /isolate="2201S31241"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-01"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            complement(315..>407)
                     /locus_tag="ACHOSC_RS27720"
                     /old_locus_tag="pgaptmp_005544"
     CDS             complement(315..>407)
                     /locus_tag="ACHOSC_RS27720"
                     /old_locus_tag="pgaptmp_005544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sel1 repeat family protein"
                     /protein_id="HGU4551236.1"
                     /translation="VNNGNIDAKSKLQELKKLKRMDRRKNKENP"
BASE COUNT          116 a           94 c           67 g          130 t
ORIGIN      
        1 ttttacattg catattatta ccagaagcga ctggaatact aagcacatca taaaaattat
       61 catcacatgg tatatctgaa acacacgaaa gcatacctaa agttatactg tataaacagg
      121 tggctaatcc caaggggatc gattccagaa atcgggttca acggatactg ataaaaattc
      181 cacctacctt ccagcccaat cggagcctga gtgttatacc gcccctgctg cggttaatgt
      241 taccgataat agttatagta cagccctgat tcatcatcat actactgctg cggcagtctg
      301 atgaactatt gcttttacgg attctcctta ttcttccttc tatccattcg ctttagcttt
      361 ttgagctctt gaagtttgct ctttgcatca atattaccgt tatttac
//
LOCUS       DAZKHF020000831          405 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2201S31241
            SAMN43267800-rid25106413.denovo.836, whole genome shotgun sequence.
ACCESSION   DAZKHF020000831 DAZKHF020000000
VERSION     DAZKHF020000831.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267800
            Sequence Read Archive: SRR30307263
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 405)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 405)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 405)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350581.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350581.2
            Genome Coverage       :: 151x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:21:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,652
            CDSs (total)                      :: 5,557
            Genes (coding)                    :: 5,320
            CDSs (with protein)               :: 5,320
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 237
            CDSs (without protein)            :: 237
            Pseudo Genes (ambiguous residues) :: 0 of 237
            Pseudo Genes (frameshifted)       :: 102 of 237
            Pseudo Genes (incomplete)         :: 130 of 237
            Pseudo Genes (internal stop)      :: 52 of 237
            Pseudo Genes (multiple problems)  :: 41 of 237
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..405
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267800-rid25106413.denovo.836"
                     /serotype="O 26:H11"
                     /isolate="2201S31241"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-01"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            8..>405
                     /locus_tag="ACHOSC_RS27725"
                     /old_locus_tag="pgaptmp_005545"
     CDS             8..>405
                     /locus_tag="ACHOSC_RS27725"
                     /old_locus_tag="pgaptmp_005545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417323.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HGU4551237.1"
                     /translation="MGLCSRYKSLTCNSCSMHCQIMPEESPRLQYCANSCFCMWPEES
                     SYFNRGVVEGILTKNHNARLSGYIFVDFSVSFLRLFLEKDWIDYLASTDMGIVLVSDR
                     NMQSLANYWRKHNSAISAVIYNDDGLDVAN"
BASE COUNT          119 a           69 c           86 g          131 t
ORIGIN      
        1 tcttaacatg gggctttgta gtcgttataa aagtcttaca tgcaatagtt gctcaatgca
       61 ttgccaaata atgccagaag agtcaccgcg tttgcagtac tgcgcaaact cgtgtttttg
      121 tatgtggccc gaagaaagtt catattttaa tcgtggtgtg gtagaaggta tcttaacaaa
      181 aaaccacaat gcgagattaa gcggatatat ttttgtcgat ttttcagtaa gttttttacg
      241 tctattcctt gaaaaagact ggattgacta tcttgcaagt actgacatgg ggattgtttt
      301 agtcagcgac cgcaatatgc aatcgttagc taattactgg cgaaaacaca actcagcaat
      361 atctgctgtc atatataatg atgatggtct tgatgttgcc aatga
//
LOCUS       DAZKHF020000832          403 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2201S31241
            SAMN43267800-rid25106413.denovo.837, whole genome shotgun sequence.
ACCESSION   DAZKHF020000832 DAZKHF020000000
VERSION     DAZKHF020000832.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267800
            Sequence Read Archive: SRR30307263
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 403)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 403)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 403)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350581.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350581.2
            Genome Coverage       :: 151x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:21:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,652
            CDSs (total)                      :: 5,557
            Genes (coding)                    :: 5,320
            CDSs (with protein)               :: 5,320
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 237
            CDSs (without protein)            :: 237
            Pseudo Genes (ambiguous residues) :: 0 of 237
            Pseudo Genes (frameshifted)       :: 102 of 237
            Pseudo Genes (incomplete)         :: 130 of 237
            Pseudo Genes (internal stop)      :: 52 of 237
            Pseudo Genes (multiple problems)  :: 41 of 237
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..403
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267800-rid25106413.denovo.837"
                     /serotype="O 26:H11"
                     /isolate="2201S31241"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-01"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            91..>403
                     /locus_tag="ACHOSC_RS27730"
                     /old_locus_tag="pgaptmp_005546"
     CDS             91..>403
                     /locus_tag="ACHOSC_RS27730"
                     /old_locus_tag="pgaptmp_005546"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4551238.1"
                     /translation="MTDREQPDYRNSIKESISAIESLCRKITGNDKGTLGACLKAIEE
                     KGYIHSAMKGAFSQLYGYTSDQGGIRHALTEEDVNPTLAEAKFMLVTCSAFSNYLLSK
                     IS"
BASE COUNT          137 a           67 c           92 g          107 t
ORIGIN      
        1 tcaaatgata acgaaattga aagtgttcag aacgcagttg ataatggcac tgattcatcg
       61 cgtagccatt tcgaaagggc gttacaacta atgacggata gagagcagcc agactacaga
      121 aactcgatta aagagtctat ctctgcaatt gaatctttat gcaggaaaat cacgggcaat
      181 gataaaggaa ccttgggggc ttgtcttaaa gctatagagg aaaaaggata tatacactct
      241 gcaatgaaag gcgcttttag tcagctctat ggttatacaa gtgatcaggg tggaatacgt
      301 catgcgctaa cagaagaaga tgtaaatcct accttagctg aagctaaatt tatgttagtc
      361 acttgcagtg cgttcagtaa ttatctgttg tcaaaaataa gtg
//
LOCUS       DAZKHF020000833          401 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2201S31241
            SAMN43267800-rid25106413.denovo.838, whole genome shotgun sequence.
ACCESSION   DAZKHF020000833 DAZKHF020000000
VERSION     DAZKHF020000833.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267800
            Sequence Read Archive: SRR30307263
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 401)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 401)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 401)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350581.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350581.2
            Genome Coverage       :: 151x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:21:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,652
            CDSs (total)                      :: 5,557
            Genes (coding)                    :: 5,320
            CDSs (with protein)               :: 5,320
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 237
            CDSs (without protein)            :: 237
            Pseudo Genes (ambiguous residues) :: 0 of 237
            Pseudo Genes (frameshifted)       :: 102 of 237
            Pseudo Genes (incomplete)         :: 130 of 237
            Pseudo Genes (internal stop)      :: 52 of 237
            Pseudo Genes (multiple problems)  :: 41 of 237
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..401
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267800-rid25106413.denovo.838"
                     /serotype="O 26:H11"
                     /isolate="2201S31241"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-01"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            <1..357
                     /locus_tag="ACHOSC_RS27735"
                     /old_locus_tag="pgaptmp_005547"
     CDS             <1..357
                     /locus_tag="ACHOSC_RS27735"
                     /old_locus_tag="pgaptmp_005547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309145.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tail assembly protein"
                     /protein_id="HGU4551239.1"
                     /translation="AVIVGSFFTAGASMALWGSALAAGGFSATTMLFSLGASMILGGV
                     AQMLAPKAKVPEYKSTDNGRQNTYFSSLDNMIAQGNPMPVPYGEMLVGSRRISQDIST
                     RDEGGDGKVVVIGRQA"
BASE COUNT           86 a           99 c          132 g           84 t
ORIGIN      
        1 gcagtcatcg tggggtcgtt cttcacggcc ggggcatcaa tggcgttatg gggttcagcc
       61 ctggcagccg gtggtttttc tgccaccacg atgctgtttt ctctgggggc cagcatgatt
      121 ctgggtggtg tggctcagat gctggccccg aaggctaaag taccggagta caaaagcacg
      181 gataacggca gacagaacac gtacttttcc tcgctggata acatgattgc ccaggggaac
      241 ccgatgccgg tgccttacgg tgaaatgctg gttggctccc gccgtatatc ccaggacatc
      301 agtacccgtg atgaaggcgg tgacgggaag gtggtggtta tcgggcggca ggcataaaag
      361 cgaaaaaatc ccgcagtgct cacggacagg aactgcggga g
//
LOCUS       DAZKHF020000834          400 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2201S31241
            SAMN43267800-rid25106413.denovo.839, whole genome shotgun sequence.
ACCESSION   DAZKHF020000834 DAZKHF020000000
VERSION     DAZKHF020000834.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267800
            Sequence Read Archive: SRR30307263
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 400)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 400)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 400)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350581.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350581.2
            Genome Coverage       :: 151x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:21:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,652
            CDSs (total)                      :: 5,557
            Genes (coding)                    :: 5,320
            CDSs (with protein)               :: 5,320
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 237
            CDSs (without protein)            :: 237
            Pseudo Genes (ambiguous residues) :: 0 of 237
            Pseudo Genes (frameshifted)       :: 102 of 237
            Pseudo Genes (incomplete)         :: 130 of 237
            Pseudo Genes (internal stop)      :: 52 of 237
            Pseudo Genes (multiple problems)  :: 41 of 237
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..400
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267800-rid25106413.denovo.839"
                     /serotype="O 26:H11"
                     /isolate="2201S31241"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-01"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            complement(56..346)
                     /locus_tag="ACHOSC_RS27740"
                     /old_locus_tag="pgaptmp_005548"
     CDS             complement(56..346)
                     /locus_tag="ACHOSC_RS27740"
                     /old_locus_tag="pgaptmp_005548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein ren"
                     /protein_id="HGU4551240.1"
                     /translation="MTGKEAIIHYLGTHNSFCAPDVAALTGATVTSINQAAAKMARAG
                     LLVIEGKVWRTVYYRFATKEEREGKMSTNLIFKECRQSAAMKRVLAVYGVKR"
BASE COUNT           86 a          116 c           82 g          116 t
ORIGIN      
        1 ataaaaaggc ctgcgattac cagcaggcct gttattagct cagtgatgta gatggtcatc
       61 ttttaactcc atataccgcc aatacccgtt tcatcgcggc actctggcga cactccttaa
      121 aaattaggtt cgtgctcatc tttccttccc gttcttcctt ggtagcaaac cggtaataca
      181 ccgttcgcca gaccttacct tcgataacca gaagacctgc ccgtgccatt ttagccgcgg
      241 cctgatttat gctggttact gttgcgcctg ttagcgcggc aacgtccggc gcacagaagc
      301 tattatgcgt ccccaggtaa tgaataattg cctctttgcc cgtcatacac ttgctccttt
      361 cagtccgaac ttagctttga gttctgcgat cttcgccaga
//
LOCUS       DAZKHF020000835          398 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2201S31241
            SAMN43267800-rid25106413.denovo.840, whole genome shotgun sequence.
ACCESSION   DAZKHF020000835 DAZKHF020000000
VERSION     DAZKHF020000835.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267800
            Sequence Read Archive: SRR30307263
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 398)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 398)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 398)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350581.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350581.2
            Genome Coverage       :: 151x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:21:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,652
            CDSs (total)                      :: 5,557
            Genes (coding)                    :: 5,320
            CDSs (with protein)               :: 5,320
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 237
            CDSs (without protein)            :: 237
            Pseudo Genes (ambiguous residues) :: 0 of 237
            Pseudo Genes (frameshifted)       :: 102 of 237
            Pseudo Genes (incomplete)         :: 130 of 237
            Pseudo Genes (internal stop)      :: 52 of 237
            Pseudo Genes (multiple problems)  :: 41 of 237
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..398
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267800-rid25106413.denovo.840"
                     /serotype="O 26:H11"
                     /isolate="2201S31241"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-01"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            complement(<1..397)
                     /locus_tag="ACHOSC_RS27745"
                     /old_locus_tag="pgaptmp_005549"
     CDS             complement(<1..397)
                     /locus_tag="ACHOSC_RS27745"
                     /old_locus_tag="pgaptmp_005549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T3SS effector protein Map"
                     /protein_id="HGU4551241.1"
                     /translation="MTMVGRSLAQAATQTLRPAVTRAATQAGVGASGMKFVPMQSNFM
                     INHGKLTNQLLQAVAKQTRSGDTQQWFKQEQITYISRTVNRAIDDYCMSNNSVIGKET
                     KSHIFKAVESAIKQPLDMNCAQTSIGHFLQ"
BASE COUNT           92 a           95 c           86 g          125 t
ORIGIN      
        1 actgtaagaa atggccaatc gacgtttgtg cacaattcat atcgagaggc tgctttatcg
       61 cgctttcaac agccttgaaa atgtggcttt tggtttcctt accaattacc gaattattac
      121 tcatgcaata gtcatcaata gctctgttta ctgttctgga tatataagta atttgctcct
      181 gtttgaacca ttgctgagta tcaccgctac gggtttgttt ggccacggcc tgaagtagct
      241 ggttagtcag tttgccatga ttaatcataa agttcgactg catgggcacg aacttcatgc
      301 cactggctcc cacccccgct tgtgtcgcag ctctggtgac cgcaggtcta agagtttgtg
      361 tagccgcctg agctaacgat ctacctacca ttgtcatt
//
LOCUS       DAZKHF020000836          395 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2201S31241
            SAMN43267800-rid25106413.denovo.841, whole genome shotgun sequence.
ACCESSION   DAZKHF020000836 DAZKHF020000000
VERSION     DAZKHF020000836.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267800
            Sequence Read Archive: SRR30307263
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 395)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 395)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 395)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350581.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350581.2
            Genome Coverage       :: 151x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:21:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,652
            CDSs (total)                      :: 5,557
            Genes (coding)                    :: 5,320
            CDSs (with protein)               :: 5,320
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 237
            CDSs (without protein)            :: 237
            Pseudo Genes (ambiguous residues) :: 0 of 237
            Pseudo Genes (frameshifted)       :: 102 of 237
            Pseudo Genes (incomplete)         :: 130 of 237
            Pseudo Genes (internal stop)      :: 52 of 237
            Pseudo Genes (multiple problems)  :: 41 of 237
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..395
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267800-rid25106413.denovo.841"
                     /serotype="O 26:H11"
                     /isolate="2201S31241"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-01"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            20..322
                     /locus_tag="ACHOSC_RS27750"
                     /old_locus_tag="pgaptmp_005550"
     CDS             20..322
                     /locus_tag="ACHOSC_RS27750"
                     /old_locus_tag="pgaptmp_005550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000338347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SepZ/EspZ family type III secretion system
                     protein"
                     /protein_id="HGU4551242.1"
                     /translation="MDAANLSPSGGVLPLAATINGNVSVDENTGVMTPENGVSRNIRI
                     AAGIALATTALAALGTGIAMACTTDASSQEFLGLGIATGVLGGVTALGGGLAMKYA"
BASE COUNT          122 a           74 c           98 g          101 t
ORIGIN      
        1 agacaatgga gaaaagttaa tggatgcagc aaatttaagt ccttctggcg gagtattacc
       61 actggcagcc acaatcaatg gaaatgtttc ggtggatgaa aatacaggag taatgacacc
      121 agaaaatggg gtaagtcgta atatcagaat tgcagccgga attgcactcg ctactacagc
      181 tctggctgct ttaggtactg gtatcgcaat ggcatgcaca acagatgcaa gctcccaaga
      241 attcttaggt ttaggtattg ctaccggtgt attaggtggt gttactgcgc ttggcggcgg
      301 attagcgatg aaatatgcct aatcatcgca tcgcataaat aaaatagaga ggtcatggat
      361 gcattatgcc attacctctc tcatgaaaag agact
//
LOCUS       DAZKHF020000837          393 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2201S31241
            SAMN43267800-rid25106413.denovo.842, whole genome shotgun sequence.
ACCESSION   DAZKHF020000837 DAZKHF020000000
VERSION     DAZKHF020000837.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267800
            Sequence Read Archive: SRR30307263
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 393)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 393)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 393)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350581.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350581.2
            Genome Coverage       :: 151x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:21:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,652
            CDSs (total)                      :: 5,557
            Genes (coding)                    :: 5,320
            CDSs (with protein)               :: 5,320
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 237
            CDSs (without protein)            :: 237
            Pseudo Genes (ambiguous residues) :: 0 of 237
            Pseudo Genes (frameshifted)       :: 102 of 237
            Pseudo Genes (incomplete)         :: 130 of 237
            Pseudo Genes (internal stop)      :: 52 of 237
            Pseudo Genes (multiple problems)  :: 41 of 237
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..393
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267800-rid25106413.denovo.842"
                     /serotype="O 26:H11"
                     /isolate="2201S31241"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-01"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            complement(<1..123)
                     /locus_tag="ACHOSC_RS27755"
                     /old_locus_tag="pgaptmp_005551"
     CDS             complement(<1..123)
                     /locus_tag="ACHOSC_RS27755"
                     /old_locus_tag="pgaptmp_005551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000581608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HGU4551243.1"
                     /translation="MIHLVNKLINFGFTRTDAQVYISLLKNGRSSGYKIAKEISL"
BASE COUNT          123 a           72 c           69 g          129 t
ORIGIN      
        1 aagtgaaatt tcttttgcta tcttgtaacc actgctacga ccgtttttca acagactgat
       61 gtaaacctgt gcatcggtgc gagtgaaacc aaaatttatc aatttattta ccaaatgtat
      121 catagacgcg ctcctgaaat gaaaatgaaa tcttaccaat tttaacatta ttaattaaca
      181 ttttcgaaaa aattgctgag ctaaaatgtg gatttatcgt aattcatcct caagctggtt
      241 acaggtctac agtacgtaag cattgtgaac tgcgaatctg aatgccctcc aactatggtc
      301 agaagacatg ctctgaccta aatagtaggc tcactgagta agcggtgtta tcaacggttt
      361 attcatatat gctctttttt atggatggaa ttt
//
LOCUS       DAZKHF020000838          393 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2201S31241
            SAMN43267800-rid25106413.denovo.843, whole genome shotgun sequence.
ACCESSION   DAZKHF020000838 DAZKHF020000000
VERSION     DAZKHF020000838.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267800
            Sequence Read Archive: SRR30307263
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 393)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 393)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 393)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350581.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350581.2
            Genome Coverage       :: 151x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:21:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,652
            CDSs (total)                      :: 5,557
            Genes (coding)                    :: 5,320
            CDSs (with protein)               :: 5,320
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 237
            CDSs (without protein)            :: 237
            Pseudo Genes (ambiguous residues) :: 0 of 237
            Pseudo Genes (frameshifted)       :: 102 of 237
            Pseudo Genes (incomplete)         :: 130 of 237
            Pseudo Genes (internal stop)      :: 52 of 237
            Pseudo Genes (multiple problems)  :: 41 of 237
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..393
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267800-rid25106413.denovo.843"
                     /serotype="O 26:H11"
                     /isolate="2201S31241"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-01"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            complement(<1..94)
                     /locus_tag="ACHOSC_RS27760"
                     /old_locus_tag="pgaptmp_005552"
     CDS             complement(<1..94)
                     /locus_tag="ACHOSC_RS27760"
                     /old_locus_tag="pgaptmp_005552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000491542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="secretion protein EspS"
                     /protein_id="HGU4551244.1"
                     /translation="MFSIRTLLPISASVSVPTKQSQSIPITLAGR"
     gene            complement(235..>393)
                     /locus_tag="ACHOSC_RS27765"
                     /old_locus_tag="pgaptmp_005553"
     CDS             complement(235..>393)
                     /locus_tag="ACHOSC_RS27765"
                     /old_locus_tag="pgaptmp_005553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311516.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail fiber C-terminal domain-containing
                     protein"
                     /protein_id="HGU4551245.1"
                     /translation="LESDENGIIPEQARVITQVVILDADKKQIQCVVRPLQILRADGT
                     WENIGGMK"
BASE COUNT           96 a          102 c           77 g          118 t
ORIGIN      
        1 ttctccctgc taaagttatt gggatggatt gagattgttt tgtcggaact gatacgctgg
       61 cgctaatagg tagtagggtt cttatgctaa acatacaacc tctttcttaa ttttcgaacc
      121 tgtttaggat tctgtgtaaa ttcaaaataa acctgtctga acattcctaa caaacatcca
      181 ccggacatga caacaaaaac cggagccgga ctccggtttt tgtgaagctg tcggctattt
      241 cattccgcca atattttccc acgtcccgtc agcacgcaga atttgcagcg gtcttaccac
      301 gcactgtatc tgctttttat ccgcatccag tatcaccacc tgcgtgatta ccctggcctg
      361 ctccgggata atgccattct catcggactc cag
//
LOCUS       DAZKHF020000839          388 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2201S31241
            SAMN43267800-rid25106413.denovo.844, whole genome shotgun sequence.
ACCESSION   DAZKHF020000839 DAZKHF020000000
VERSION     DAZKHF020000839.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267800
            Sequence Read Archive: SRR30307263
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 388)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 388)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 388)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350581.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350581.2
            Genome Coverage       :: 151x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:21:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,652
            CDSs (total)                      :: 5,557
            Genes (coding)                    :: 5,320
            CDSs (with protein)               :: 5,320
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 237
            CDSs (without protein)            :: 237
            Pseudo Genes (ambiguous residues) :: 0 of 237
            Pseudo Genes (frameshifted)       :: 102 of 237
            Pseudo Genes (incomplete)         :: 130 of 237
            Pseudo Genes (internal stop)      :: 52 of 237
            Pseudo Genes (multiple problems)  :: 41 of 237
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..388
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267800-rid25106413.denovo.844"
                     /serotype="O 26:H11"
                     /isolate="2201S31241"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-01"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            7..>388
                     /locus_tag="ACHOSC_RS27770"
                     /old_locus_tag="pgaptmp_005554"
     CDS             7..>388
                     /locus_tag="ACHOSC_RS27770"
                     /old_locus_tag="pgaptmp_005554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4551246.1"
                     /translation="MNIQPIVTSGITTQNNRHHHAEQTSPTQIPQSELPNGCETGFVV
                     HIPEDMQRHAPECGETTALLSLIKDEGLLSGLDKYLAPHLEEGSAGKKALDMFGLFNV
                     SQMALEIPSTVPGISGKYGVQLNIV"
BASE COUNT          125 a           89 c           78 g           96 t
ORIGIN      
        1 tttatcatga acattcaacc gatcgtaaca tccggaatca ccacacaaaa caatcgacat
       61 catcacgcag aacaaacgtc ccctacacaa ataccgcaat ccgaattacc taatggatgc
      121 gaaacgggat ttgttgttca tatcccagag gatatgcagc gacatgcacc ggaatgcggt
      181 gaaacaacag ctctactgag cttgataaaa gatgaaggtc tgctctctgg gctggataaa
      241 tatcttgcac ctcatcttga agaaggctct gcaggaaaaa aagcattgga tatgtttggt
      301 ttattcaatg tctctcagat ggcattagaa atacccagca ccgttccggg tatctctggt
      361 aaatatggtg tccagctaaa cattgtaa
//
LOCUS       DAZKHF020000840          385 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2201S31241
            SAMN43267800-rid25106413.denovo.845, whole genome shotgun sequence.
ACCESSION   DAZKHF020000840 DAZKHF020000000
VERSION     DAZKHF020000840.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267800
            Sequence Read Archive: SRR30307263
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 385)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 385)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 385)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350581.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350581.2
            Genome Coverage       :: 151x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:21:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,652
            CDSs (total)                      :: 5,557
            Genes (coding)                    :: 5,320
            CDSs (with protein)               :: 5,320
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 237
            CDSs (without protein)            :: 237
            Pseudo Genes (ambiguous residues) :: 0 of 237
            Pseudo Genes (frameshifted)       :: 102 of 237
            Pseudo Genes (incomplete)         :: 130 of 237
            Pseudo Genes (internal stop)      :: 52 of 237
            Pseudo Genes (multiple problems)  :: 41 of 237
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..385
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267800-rid25106413.denovo.845"
                     /serotype="O 26:H11"
                     /isolate="2201S31241"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-01"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            complement(<1..293)
                     /locus_tag="ACHOSC_RS27775"
                     /old_locus_tag="pgaptmp_005555"
     CDS             complement(<1..293)
                     /locus_tag="ACHOSC_RS27775"
                     /old_locus_tag="pgaptmp_005555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001519435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-packaging protein"
                     /protein_id="HGU4551247.1"
                     /translation="MAFLLNKSDMASSIGISVQAFDKWGVPPVERRGREVFYDVKTVL
                     EIDRERRQHNQRIPDDEGDLEERLLRARAELTEEQAVAQKLKNQVTEGKLIDTG"
BASE COUNT          100 a          104 c           67 g          114 t
ORIGIN      
        1 ccggtgtcaa taagcttacc ttcggttacc tgatttttaa gtttttgagc tacggcctgt
       61 tcttctgtca gttcagctct ggcccgaagc agcctttcct ccagatcgcc ctcgtcatca
      121 ggtattctct gattgtgttg tcgccgctcg cgatctatct ccagtacagt tttaacgtca
      181 taaaaaactt ccctcccccg acgctcaaca ggaggaacgc cccatttatc aaatgcctga
      241 acagatatac cgatggagga ggccatatca cttttattca ataaaaaggc catctcctct
      301 ccataagcca tcgataaaaa gcgatacaac aaccatgtgt ttttacaaaa ccatttgata
      361 tcattgacat ttttcgcatt gacga
//
LOCUS       DAZKHF020000841          378 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2201S31241
            SAMN43267800-rid25106413.denovo.846, whole genome shotgun sequence.
ACCESSION   DAZKHF020000841 DAZKHF020000000
VERSION     DAZKHF020000841.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267800
            Sequence Read Archive: SRR30307263
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 378)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 378)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 378)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350581.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350581.2
            Genome Coverage       :: 151x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:21:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,652
            CDSs (total)                      :: 5,557
            Genes (coding)                    :: 5,320
            CDSs (with protein)               :: 5,320
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 237
            CDSs (without protein)            :: 237
            Pseudo Genes (ambiguous residues) :: 0 of 237
            Pseudo Genes (frameshifted)       :: 102 of 237
            Pseudo Genes (incomplete)         :: 130 of 237
            Pseudo Genes (internal stop)      :: 52 of 237
            Pseudo Genes (multiple problems)  :: 41 of 237
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..378
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267800-rid25106413.denovo.846"
                     /serotype="O 26:H11"
                     /isolate="2201S31241"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-01"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            complement(80..289)
                     /gene="fumD"
                     /locus_tag="ACHOSC_RS27780"
                     /old_locus_tag="pgaptmp_005556"
     CDS             complement(80..289)
                     /gene="fumD"
                     /locus_tag="ACHOSC_RS27780"
                     /old_locus_tag="pgaptmp_005556"
                     /EC_number="4.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate hydratase FumD"
                     /protein_id="HGU4551248.1"
                     /translation="MGNRTKEDELYREMCRVVGKVVLEMRDLGQEPKHIVIAGVLRTA
                     LANKRIQRSELEKQAMETVINALVK"
BASE COUNT          108 a           96 c           60 g          114 t
ORIGIN      
        1 gttgttattt agaatgatta actggaccgc aactgaagca agaatatcag gatgaagctt
       61 accgcctcat cctgaatggt cattttacca gtgcgttaat gacggtttcc atcgcctgct
      121 tttccagctc actgcgctga atgcgtttat tggctaatgc tgtacgtaat acgcctgcga
      181 taacaatatg ctttggttcc tgccccagat cgcgcatttc cagcacaact ttaccgacaa
      241 ctctgcacat ttctcggtac agttcatcct cttttgtccg atttcccatt ctttatacct
      301 atttatcatg ccaactatca gcatatatca atctaaccaa ttaaacaaaa atcaaacaaa
      361 atcagacaaa taacgcga
//
LOCUS       DAZKHF020000842          377 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2201S31241
            SAMN43267800-rid25106413.denovo.847, whole genome shotgun sequence.
ACCESSION   DAZKHF020000842 DAZKHF020000000
VERSION     DAZKHF020000842.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267800
            Sequence Read Archive: SRR30307263
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 377)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 377)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 377)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350581.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350581.2
            Genome Coverage       :: 151x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:21:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,652
            CDSs (total)                      :: 5,557
            Genes (coding)                    :: 5,320
            CDSs (with protein)               :: 5,320
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 237
            CDSs (without protein)            :: 237
            Pseudo Genes (ambiguous residues) :: 0 of 237
            Pseudo Genes (frameshifted)       :: 102 of 237
            Pseudo Genes (incomplete)         :: 130 of 237
            Pseudo Genes (internal stop)      :: 52 of 237
            Pseudo Genes (multiple problems)  :: 41 of 237
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..377
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267800-rid25106413.denovo.847"
                     /serotype="O 26:H11"
                     /isolate="2201S31241"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-01"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            <1..>377
                     /locus_tag="ACHOSC_RS27785"
                     /old_locus_tag="pgaptmp_005557"
     CDS             <1..>377
                     /locus_tag="ACHOSC_RS27785"
                     /old_locus_tag="pgaptmp_005557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage major capsid protein"
                     /protein_id="HGU4551249.1"
                     /translation="TDFVDPKKAAVADVSPASITHDVKGTASTGNPDADAEAAFGQFV
                     TANLQPTGAVWLMSSTNALALSMRKNALGQKEYPDMTLLGGTFQGLPVIVSQYVGDQL
                     VLVNAPDIYLADDGGVAVDMSREA"
BASE COUNT           73 a          100 c          127 g           77 t
ORIGIN      
        1 acagactttg tggacccgaa aaaagcggcg gtggcggatg tctccccggc gtccatcacc
       61 catgatgtga agggcacggc atcaaccggt aacccggatg cggatgccga ggccgcgttt
      121 ggccagtttg tgacggcaaa tctgcagccc accggtgcgg tctggctgat gtccagcacc
      181 aatgccctgg cgctgtccat gcgtaaaaat gcgctggggc agaaggaata tccggacatg
      241 accctgctgg gcgggacctt ccaggggctg ccggtgattg tctcccagta tgtgggtgac
      301 cagctggtac tggtgaatgc accggatatt tatctggctg atgacggcgg tgtggcggtg
      361 gatatgtccc gtgaagc
//
LOCUS       DAZKHF020000843          376 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2201S31241
            SAMN43267800-rid25106413.denovo.848, whole genome shotgun sequence.
ACCESSION   DAZKHF020000843 DAZKHF020000000
VERSION     DAZKHF020000843.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267800
            Sequence Read Archive: SRR30307263
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 376)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 376)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 376)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350581.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350581.2
            Genome Coverage       :: 151x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:21:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,652
            CDSs (total)                      :: 5,557
            Genes (coding)                    :: 5,320
            CDSs (with protein)               :: 5,320
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 237
            CDSs (without protein)            :: 237
            Pseudo Genes (ambiguous residues) :: 0 of 237
            Pseudo Genes (frameshifted)       :: 102 of 237
            Pseudo Genes (incomplete)         :: 130 of 237
            Pseudo Genes (internal stop)      :: 52 of 237
            Pseudo Genes (multiple problems)  :: 41 of 237
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..376
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267800-rid25106413.denovo.848"
                     /serotype="O 26:H11"
                     /isolate="2201S31241"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-01"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            276..376
                     /locus_tag="ACHOSC_RS27790"
                     /old_locus_tag="pgaptmp_005558"
                     /pseudo
     CDS             276..376
                     /locus_tag="ACHOSC_RS27790"
                     /old_locus_tag="pgaptmp_005558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477181.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS1 family transposase"
BASE COUNT           90 a          104 c           69 g          113 t
ORIGIN      
        1 tcaattttta aacataaaat gcgaattatt tagtacaaaa agtgaacttt tgatgatctt
       61 tccctaatct cacataggat cagatctcgt ctgatcgttc cccctgcgct cactaccatg
      121 gctctttacc acagcagccg gggttgtttg ggaccgactt ccgacagcag atcccggtgg
      181 gcctgccacc atctcttcac aagcttatgc ttattacatg caaaacgtcc cgttttgcat
      241 ctttgtgcct acggcatccc tgtgatcttt ccctgaatct gcccctgaga acctgaatca
      301 aacgtctgac tcgcagaacc atctgtttct cgcgctctgt agaacttcat gaaaaagtca
      361 tcggtgcttt tatcga
//
LOCUS       DAZKHF020000844          369 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2201S31241
            SAMN43267800-rid25106413.denovo.849, whole genome shotgun sequence.
ACCESSION   DAZKHF020000844 DAZKHF020000000
VERSION     DAZKHF020000844.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267800
            Sequence Read Archive: SRR30307263
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 369)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 369)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 369)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350581.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350581.2
            Genome Coverage       :: 151x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:21:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,652
            CDSs (total)                      :: 5,557
            Genes (coding)                    :: 5,320
            CDSs (with protein)               :: 5,320
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 237
            CDSs (without protein)            :: 237
            Pseudo Genes (ambiguous residues) :: 0 of 237
            Pseudo Genes (frameshifted)       :: 102 of 237
            Pseudo Genes (incomplete)         :: 130 of 237
            Pseudo Genes (internal stop)      :: 52 of 237
            Pseudo Genes (multiple problems)  :: 41 of 237
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..369
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267800-rid25106413.denovo.849"
                     /serotype="O 26:H11"
                     /isolate="2201S31241"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-01"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            <1..>369
                     /locus_tag="ACHOSC_RS27795"
                     /old_locus_tag="pgaptmp_005559"
     CDS             <1..>369
                     /locus_tag="ACHOSC_RS27795"
                     /old_locus_tag="pgaptmp_005559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ShET2/EspL2 family type III secretion system
                     effector toxin"
                     /protein_id="HGU4551250.1"
                     /translation="AAQYINDIYQNVDYKPHQDDYSSAEKFLTHFNKKCKNQTLALIS
                     SRPEGRCVAACGDFGLVMKAYFDKMESNGISVMAAILLVDNHALTVRLRIKNTTEGCT
                     HYVVSVYDPNVTNDKIRIMS"
BASE COUNT          114 a           69 c           78 g          108 t
ORIGIN      
        1 ttgctgcaca atatattaat gatatttatc agaatgttga ttacaaaccc catcaagatg
       61 attactcttc agctgaaaaa tttctcactc acttcaacaa aaaatgcaaa aaccagactt
      121 tggcgttgat ttccagccgt cctgaggggc gttgtgttgc tgcctgtggt gatttcgggc
      181 tagttatgaa ggcatatttc gacaagatgg aatcaaatgg catcagtgtt atggcagcca
      241 tattactggt ggataaccat gctttgacgg tccggctaag aataaagaac acaactgaag
      301 gatgtaccca ttacgtggtt tcggtttatg atcctaatgt cactaacgat aaaataagaa
      361 ttatgagcg
//
LOCUS       DAZKHF020000845          365 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2201S31241
            SAMN43267800-rid25106413.denovo.850, whole genome shotgun sequence.
ACCESSION   DAZKHF020000845 DAZKHF020000000
VERSION     DAZKHF020000845.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267800
            Sequence Read Archive: SRR30307263
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 365)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 365)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 365)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350581.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350581.2
            Genome Coverage       :: 151x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:21:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,652
            CDSs (total)                      :: 5,557
            Genes (coding)                    :: 5,320
            CDSs (with protein)               :: 5,320
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 237
            CDSs (without protein)            :: 237
            Pseudo Genes (ambiguous residues) :: 0 of 237
            Pseudo Genes (frameshifted)       :: 102 of 237
            Pseudo Genes (incomplete)         :: 130 of 237
            Pseudo Genes (internal stop)      :: 52 of 237
            Pseudo Genes (multiple problems)  :: 41 of 237
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..365
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267800-rid25106413.denovo.850"
                     /serotype="O 26:H11"
                     /isolate="2201S31241"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-01"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            <1..>365
                     /locus_tag="ACHOSC_RS27800"
                     /old_locus_tag="pgaptmp_005560"
     CDS             <1..>365
                     /locus_tag="ACHOSC_RS27800"
                     /old_locus_tag="pgaptmp_005560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_304285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="phage tail tape measure protein"
                     /protein_id="HGU4551251.1"
                     /translation="NALEAWRNNEANKPKSRGGKSETEKAEDSFSRLLKQQKEQLALV
                     GQNTELAKLKYQTAQGELKTLTEMQKQELLRNAALIDQQKIREQLRSREETLKNENAA
                     ARASNDAELLGYGQGERAR"
BASE COUNT          112 a           81 c          122 g           50 t
ORIGIN      
        1 taatgccctg gaagcctggc gcaataacga ggcgaataaa cccaaatccc ggggtggaaa
       61 atcagagacg gaaaaagcgg aagacagttt ttcccggctg ctgaagcagc agaaagagca
      121 actggcactg gtggggcaga atacagagct ggcgaagctg aaataccaga ctgcgcaggg
      181 cgaactgaaa accctgacgg agatgcagaa gcaggagctg ctgcgtaatg cggccctgat
      241 tgaccagcaa aaaatccggg aacagttgcg atcccgggaa gagacactga agaatgagaa
      301 tgcggctgcg cgtgcgtcga atgatgctga actgctgggg tacgggcagg gggaacgagc
      361 cagag
//
LOCUS       DAZKHF020000846          364 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2201S31241
            SAMN43267800-rid25106413.denovo.851, whole genome shotgun sequence.
ACCESSION   DAZKHF020000846 DAZKHF020000000
VERSION     DAZKHF020000846.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267800
            Sequence Read Archive: SRR30307263
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 364)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 364)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 364)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350581.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350581.2
            Genome Coverage       :: 151x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:21:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,652
            CDSs (total)                      :: 5,557
            Genes (coding)                    :: 5,320
            CDSs (with protein)               :: 5,320
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 237
            CDSs (without protein)            :: 237
            Pseudo Genes (ambiguous residues) :: 0 of 237
            Pseudo Genes (frameshifted)       :: 102 of 237
            Pseudo Genes (incomplete)         :: 130 of 237
            Pseudo Genes (internal stop)      :: 52 of 237
            Pseudo Genes (multiple problems)  :: 41 of 237
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..364
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267800-rid25106413.denovo.851"
                     /serotype="O 26:H11"
                     /isolate="2201S31241"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-01"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            173..>364
                     /locus_tag="ACHOSC_RS27805"
                     /old_locus_tag="pgaptmp_005561"
     CDS             173..>364
                     /locus_tag="ACHOSC_RS27805"
                     /old_locus_tag="pgaptmp_005561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012904434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HGU4551252.1"
                     /translation="MEHELHYIGIDTAKEKLDVDVLRPDGRHRTKKFANTTKGHDELV
                     SWLKGHKIDHAHICIEATGT"
BASE COUNT           92 a           89 c           91 g           92 t
ORIGIN      
        1 agagaaaatc ggccagtttt ctctgcctgc agtccgcatg ccgtatcggg ccttgggttc
       61 taacctgttg cgtagattta tgcagcggac tgcctttctc ccaaagtgat aaaccggaca
      121 gtatcatgga ccggttttcc cggtaatccg tatttgcaag gttggtttca ctatggaaca
      181 tgaacttcat tatatcggta tcgacaccgc taaagagaaa ctggatgtcg atgtgttgcg
      241 tcctgatggt cgtcatcgca ccaaaaaatt cgctaacacc actaaagggc acgatgagct
      301 ggtgagctgg ctgaaaggtc acaagattga ccatgcgcat atctgcatcg aagcgaccgg
      361 cacc
//
LOCUS       DAZKHF020000847          363 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2201S31241
            SAMN43267800-rid25106413.denovo.852, whole genome shotgun sequence.
ACCESSION   DAZKHF020000847 DAZKHF020000000
VERSION     DAZKHF020000847.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267800
            Sequence Read Archive: SRR30307263
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 363)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 363)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 363)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350581.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350581.2
            Genome Coverage       :: 151x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:21:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,652
            CDSs (total)                      :: 5,557
            Genes (coding)                    :: 5,320
            CDSs (with protein)               :: 5,320
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 237
            CDSs (without protein)            :: 237
            Pseudo Genes (ambiguous residues) :: 0 of 237
            Pseudo Genes (frameshifted)       :: 102 of 237
            Pseudo Genes (incomplete)         :: 130 of 237
            Pseudo Genes (internal stop)      :: 52 of 237
            Pseudo Genes (multiple problems)  :: 41 of 237
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..363
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267800-rid25106413.denovo.852"
                     /serotype="O 26:H11"
                     /isolate="2201S31241"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-01"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            19..95
                     /locus_tag="ACHOSC_RS27810"
                     /old_locus_tag="pgaptmp_005562"
     tRNA            19..95
                     /locus_tag="ACHOSC_RS27810"
                     /old_locus_tag="pgaptmp_005562"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:53..55,aa:Ile,seq:gat)
     gene            138..213
                     /locus_tag="ACHOSC_RS27815"
                     /old_locus_tag="pgaptmp_005563"
     tRNA            138..213
                     /locus_tag="ACHOSC_RS27815"
                     /old_locus_tag="pgaptmp_005563"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:171..173,aa:Ala,seq:tgc)
BASE COUNT          102 a           79 c           88 g           94 t
ORIGIN      
        1 gagcagtaaa acctctacag gcttgtagct caggtggtta gagcgcaccc ctgataaggg
       61 tgaggtcggt ggttcaagtc cactcaggcc taccaaattt gcacggcaaa tttgaagagg
      121 ttttaactac atgttatggg gctatagctc agctgggaga gcgcctgctt tgcacgcagg
      181 aggtctgcgg ttcgatcccg catagctcca ccatctctgt agtgattaaa taaaaaatac
      241 ttcagagtgt acctgcaaag gttcactgcg aagttttgct ctttaaaaat ctggatcaag
      301 ctgaaaattg aaacactgaa caacgaaagt tgttcgtgag tctctcaaat tttcgcaaca
      361 cga
//
LOCUS       DAZKHF020000848          359 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2201S31241
            SAMN43267800-rid25106413.denovo.853, whole genome shotgun sequence.
ACCESSION   DAZKHF020000848 DAZKHF020000000
VERSION     DAZKHF020000848.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267800
            Sequence Read Archive: SRR30307263
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 359)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 359)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 359)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350581.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350581.2
            Genome Coverage       :: 151x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:21:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,652
            CDSs (total)                      :: 5,557
            Genes (coding)                    :: 5,320
            CDSs (with protein)               :: 5,320
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 237
            CDSs (without protein)            :: 237
            Pseudo Genes (ambiguous residues) :: 0 of 237
            Pseudo Genes (frameshifted)       :: 102 of 237
            Pseudo Genes (incomplete)         :: 130 of 237
            Pseudo Genes (internal stop)      :: 52 of 237
            Pseudo Genes (multiple problems)  :: 41 of 237
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..359
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267800-rid25106413.denovo.853"
                     /serotype="O 26:H11"
                     /isolate="2201S31241"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-01"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            complement(<1..68)
                     /locus_tag="ACHOSC_RS27820"
                     /old_locus_tag="pgaptmp_005564"
     CDS             complement(<1..68)
                     /locus_tag="ACHOSC_RS27820"
                     /old_locus_tag="pgaptmp_005564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HGU4551253.1"
                     /translation="MVTVAELQALRQARLDLLTGKRV"
     gene            complement(52..>359)
                     /locus_tag="ACHOSC_RS27825"
                     /old_locus_tag="pgaptmp_005565"
     CDS             complement(52..>359)
                     /locus_tag="ACHOSC_RS27825"
                     /old_locus_tag="pgaptmp_005565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="terminase gpA endonuclease subunit"
                     /protein_id="HGU4551254.1"
                     /translation="YAIRFPDDPEIFSQTEAQQLVAEELVEKWEKGKMRLLWDNKKRR
                     NEALDCLVYAYAALRVSVQRWQLDLAVLAKSREEETTRPTLKELAAKLSGGVNGYSR"
BASE COUNT           72 a          118 c           81 g           88 t
ORIGIN      
        1 acccgtttac cggttaataa atcaaggcgc gcctgacgca gcgcctgcag ttcagcgact
       61 gtaaccattc actcctccgg acagcttcgc tgccagttct ttcagggttg gccgggtcgt
      121 ctcttcttcc cgggattttg ccagtacagc cagatcaagc tgccagcgtt gcacggacac
      181 acgtaatgcc gcgtaggcat acaccaggca gtccagcgct tcgttacgcc gctttttgtt
      241 atcccacagc agacgcatct ttcctttttc ccacttctcc accagctctt ccgccaccag
      301 ttgctgcgcc tctgtctgcg aaaaaatctc cggatcatca ggaaaacgga tggcatacg
//
LOCUS       DAZKHF020000849          359 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2201S31241
            SAMN43267800-rid25106413.denovo.854, whole genome shotgun sequence.
ACCESSION   DAZKHF020000849 DAZKHF020000000
VERSION     DAZKHF020000849.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267800
            Sequence Read Archive: SRR30307263
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 359)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 359)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 359)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350581.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350581.2
            Genome Coverage       :: 151x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:21:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,652
            CDSs (total)                      :: 5,557
            Genes (coding)                    :: 5,320
            CDSs (with protein)               :: 5,320
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 237
            CDSs (without protein)            :: 237
            Pseudo Genes (ambiguous residues) :: 0 of 237
            Pseudo Genes (frameshifted)       :: 102 of 237
            Pseudo Genes (incomplete)         :: 130 of 237
            Pseudo Genes (internal stop)      :: 52 of 237
            Pseudo Genes (multiple problems)  :: 41 of 237
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..359
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267800-rid25106413.denovo.854"
                     /serotype="O 26:H11"
                     /isolate="2201S31241"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-01"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            complement(133..261)
                     /gene="rhuM"
                     /locus_tag="ACHOSC_RS27830"
                     /old_locus_tag="pgaptmp_005566"
                     /pseudo
     CDS             complement(133..261)
                     /gene="rhuM"
                     /locus_tag="ACHOSC_RS27830"
                     /old_locus_tag="pgaptmp_005566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_462654.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="RhuM family protein"
BASE COUNT          112 a           71 c           96 g           80 t
ORIGIN      
        1 ttatagtcag tttttttcgc cagcggttaa agaaatagaa tagtcgtata aaaaagggtg
       61 ttctggggcg agtaaaacaa catcttcacc ttaatggcga tacagttggc tgagagccag
      121 cagggggcaa atcacctgca aataaacacg gcacatgctg gcgcgcatat cgcggatgcg
      181 ttcgaggaca gaagaaccga caggcggatt cttcagccgc tcatcgtcca tcacaaaacc
      241 ttagaccagg tattcctgaa gccgattgaa tattgagcag tagaaaatat ctggattgac
      301 agtattacaa ggggttggag aacgtcagaa gaggcatttt ggcggaagat cacaggagt
//
LOCUS       DAZKHF020000850          343 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2201S31241
            SAMN43267800-rid25106413.denovo.857, whole genome shotgun sequence.
ACCESSION   DAZKHF020000850 DAZKHF020000000
VERSION     DAZKHF020000850.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267800
            Sequence Read Archive: SRR30307263
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 343)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 343)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 343)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350581.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350581.2
            Genome Coverage       :: 151x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:21:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,652
            CDSs (total)                      :: 5,557
            Genes (coding)                    :: 5,320
            CDSs (with protein)               :: 5,320
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 237
            CDSs (without protein)            :: 237
            Pseudo Genes (ambiguous residues) :: 0 of 237
            Pseudo Genes (frameshifted)       :: 102 of 237
            Pseudo Genes (incomplete)         :: 130 of 237
            Pseudo Genes (internal stop)      :: 52 of 237
            Pseudo Genes (multiple problems)  :: 41 of 237
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..343
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267800-rid25106413.denovo.857"
                     /serotype="O 26:H11"
                     /isolate="2201S31241"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-01"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            complement(<1..>343)
                     /locus_tag="ACHOSC_RS27835"
                     /old_locus_tag="pgaptmp_005567"
     CDS             complement(<1..>343)
                     /locus_tag="ACHOSC_RS27835"
                     /old_locus_tag="pgaptmp_005567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001530666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4551255.1"
                     /translation="KNKNGNDYLSLGYYDQDDYHIQRGIRINGDSLTQYCSEKARNAS
                     AWFESSKAIMAESFATGSDHQVVNELNGERLREPNEVFKRLGRAIRYNFQVDDAKFRR
                     DNVKEIISNLFA"
BASE COUNT           91 a           71 c           56 g          125 t
ORIGIN      
        1 gcgaataaat tagaaattat ttcttttaca ttgtcgcgtc taaattttgc atcgtccact
       61 tgaaaattat atcttattgc tcgacctaaa cgtttaaaaa cttcgttagg ttctctcagt
      121 ctttccccgt tgagctcgtt tacaacctga tgatcggaac ctgttgcaaa tgattctgcc
      181 atgatagctt tactgctttc aaaccacgct gaagcattcc tggctttttc actacaatat
      241 tgggtgagac tatcaccatt aatacgaatt cctctttgaa tgtggtagtc gtcctgatcg
      301 tagtaaccga gagataagta atcatttccg tttttatttt taa
//
LOCUS       DAZKHF020000851          342 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2201S31241
            SAMN43267800-rid25106413.denovo.858, whole genome shotgun sequence.
ACCESSION   DAZKHF020000851 DAZKHF020000000
VERSION     DAZKHF020000851.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267800
            Sequence Read Archive: SRR30307263
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 342)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 342)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 342)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350581.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350581.2
            Genome Coverage       :: 151x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:21:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,652
            CDSs (total)                      :: 5,557
            Genes (coding)                    :: 5,320
            CDSs (with protein)               :: 5,320
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 237
            CDSs (without protein)            :: 237
            Pseudo Genes (ambiguous residues) :: 0 of 237
            Pseudo Genes (frameshifted)       :: 102 of 237
            Pseudo Genes (incomplete)         :: 130 of 237
            Pseudo Genes (internal stop)      :: 52 of 237
            Pseudo Genes (multiple problems)  :: 41 of 237
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..342
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267800-rid25106413.denovo.858"
                     /serotype="O 26:H11"
                     /isolate="2201S31241"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-01"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            <1..257
                     /locus_tag="ACHOSC_RS27840"
                     /old_locus_tag="pgaptmp_005568"
     CDS             <1..257
                     /locus_tag="ACHOSC_RS27840"
                     /old_locus_tag="pgaptmp_005568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309827.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="major tail subunit"
                     /protein_id="HGU4551256.1"
                     /translation="KTLTVNTGALLTMSVSVNGGTPPYKHAWKKDGQPVEGQTTDTFS
                     KANTQSGDKGAYTCEVTDSAEQPQSITSDACTVTVNGAGG"
     gene            263..>342
                     /locus_tag="ACHOSC_RS27845"
                     /old_locus_tag="pgaptmp_005569"
     CDS             263..>342
                     /locus_tag="ACHOSC_RS27845"
                     /old_locus_tag="pgaptmp_005569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail assembly chaperone"
                     /protein_id="HGU4551257.1"
                     /translation="MAKDLKTLALARLSGFRHKTVKVPEW"
BASE COUNT           96 a           74 c          106 g           66 t
ORIGIN      
        1 agaagacact taccgtgaat accggtgcgc tgctgacaat gtcagtcagt gtcaacgggg
       61 gaacgccgcc ttataaacac gcctggaaga aggatggtca gccggtagag ggacagacta
      121 ctgacacttt cagtaaagcc aatacgcagt caggtgataa gggggcttat acctgcgagg
      181 taacggattc tgcagaacag ccgcagagca ttacctctga tgcgtgtaca gtaacggtta
      241 atggtgcggg cggataaggc ttatggcaaa agatctgaaa acactggcgc tggccagact
      301 gtcggggttc cgtcataaaa cggtgaaggt gccggaatgg ag
//
LOCUS       DAZKHF020000852          341 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2201S31241
            SAMN43267800-rid25106413.denovo.859, whole genome shotgun sequence.
ACCESSION   DAZKHF020000852 DAZKHF020000000
VERSION     DAZKHF020000852.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267800
            Sequence Read Archive: SRR30307263
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 341)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 341)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 341)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350581.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350581.2
            Genome Coverage       :: 151x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:21:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,652
            CDSs (total)                      :: 5,557
            Genes (coding)                    :: 5,320
            CDSs (with protein)               :: 5,320
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 237
            CDSs (without protein)            :: 237
            Pseudo Genes (ambiguous residues) :: 0 of 237
            Pseudo Genes (frameshifted)       :: 102 of 237
            Pseudo Genes (incomplete)         :: 130 of 237
            Pseudo Genes (internal stop)      :: 52 of 237
            Pseudo Genes (multiple problems)  :: 41 of 237
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..341
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267800-rid25106413.denovo.859"
                     /serotype="O 26:H11"
                     /isolate="2201S31241"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-01"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            <1..>341
                     /locus_tag="ACHOSC_RS27850"
                     /old_locus_tag="pgaptmp_005570"
     CDS             <1..>341
                     /locus_tag="ACHOSC_RS27850"
                     /old_locus_tag="pgaptmp_005570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310088.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="type IV secretion protein Rhs"
                     /protein_id="HGU4551258.1"
                     /translation="ITGSGDLISLGGHAGLGVAFAKKKGEMLSDICIYATACGHAGIG
                     GGINAAITYSETKSLPASGVSNSVGVTVGGGVGGHFAYTYVVDVDNPESSTESVGIGA
                     GVDASVMTLAC"
BASE COUNT           84 a           52 c           99 g          106 t
ORIGIN      
        1 atattactgg cagtggagat ctaatatcat taggtggtca tgcaggactt ggcgttgcgt
       61 ttgctaaaaa gaaaggtgaa atgttatctg atatttgtat ttatgctaca gcatgcggac
      121 atgcaggaat tggtggtggg ataaatgcgg ctatcacata ttctgagacc aagtctttac
      181 ctgcatcggg agtcagcaat tcagtaggtg taacggttgg cggcggagtt ggggggcatt
      241 ttgcgtatac ttatgtagtg gatgttgata atccagaatc atcgacagaa tctgttggta
      301 tcggtgcagg tgttgacgct tcagttatga ctctggcttg t
//
LOCUS       DAZKHF020000853          339 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2201S31241
            SAMN43267800-rid25106413.denovo.860, whole genome shotgun sequence.
ACCESSION   DAZKHF020000853 DAZKHF020000000
VERSION     DAZKHF020000853.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267800
            Sequence Read Archive: SRR30307263
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 339)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 339)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 339)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350581.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350581.2
            Genome Coverage       :: 151x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:21:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,652
            CDSs (total)                      :: 5,557
            Genes (coding)                    :: 5,320
            CDSs (with protein)               :: 5,320
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 237
            CDSs (without protein)            :: 237
            Pseudo Genes (ambiguous residues) :: 0 of 237
            Pseudo Genes (frameshifted)       :: 102 of 237
            Pseudo Genes (incomplete)         :: 130 of 237
            Pseudo Genes (internal stop)      :: 52 of 237
            Pseudo Genes (multiple problems)  :: 41 of 237
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..339
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267800-rid25106413.denovo.860"
                     /serotype="O 26:H11"
                     /isolate="2201S31241"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-01"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            <1..>339
                     /locus_tag="ACHOSC_RS27855"
                     /old_locus_tag="pgaptmp_005571"
     rRNA            <1..>339
                     /locus_tag="ACHOSC_RS27855"
                     /old_locus_tag="pgaptmp_005571"
                     /product="16S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00177"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00177"
BASE COUNT           86 a           75 c          115 g           63 t
ORIGIN      
        1 agaagcaccg gctaactccg tgccagcagc cgcggtaata cggagggtgc aagcgttaat
       61 cggaattact gggcgtaaag cgcacgcagg cggtttgtta agtcagatgt gaaatccccg
      121 ggctcaacct gggaactgca tctgatactg gcaagcttga gtctcgtaga ggggggtaga
      181 attccaggtg tagcggtgaa atgcgtagag atctggagga ataccggtgg cgaaggcggc
      241 cccctggacg aagactgacg ctcaggtgcg aaagcgtggg gagcaaacag gattagatac
      301 cctggtagtc cacgccgtaa acgatgtcga cttggaggt
//
LOCUS       DAZKHF020000854          335 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2201S31241
            SAMN43267800-rid25106413.denovo.861, whole genome shotgun sequence.
ACCESSION   DAZKHF020000854 DAZKHF020000000
VERSION     DAZKHF020000854.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267800
            Sequence Read Archive: SRR30307263
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 335)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 335)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 335)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350581.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350581.2
            Genome Coverage       :: 151x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:21:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,652
            CDSs (total)                      :: 5,557
            Genes (coding)                    :: 5,320
            CDSs (with protein)               :: 5,320
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 237
            CDSs (without protein)            :: 237
            Pseudo Genes (ambiguous residues) :: 0 of 237
            Pseudo Genes (frameshifted)       :: 102 of 237
            Pseudo Genes (incomplete)         :: 130 of 237
            Pseudo Genes (internal stop)      :: 52 of 237
            Pseudo Genes (multiple problems)  :: 41 of 237
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..335
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267800-rid25106413.denovo.861"
                     /serotype="O 26:H11"
                     /isolate="2201S31241"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-01"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            <1..213
                     /locus_tag="ACHOSC_RS27860"
                     /old_locus_tag="pgaptmp_005572"
     CDS             <1..213
                     /locus_tag="ACHOSC_RS27860"
                     /old_locus_tag="pgaptmp_005572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000246335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tail assembly protein"
                     /protein_id="HGU4551259.1"
                     /translation="APKAKTPDYRAMDNGRQNTYFSSLDNMIAQGNPMPVPYGEMLVG
                     SRRISQDISTRDEGGGGKVVVIGRQR"
BASE COUNT          100 a           74 c           98 g           63 t
ORIGIN      
        1 gcaccgaagg caaaaacacc ggattaccgc gcaatggata acggcagaca gaacacgtac
       61 ttttcctcac tggacaacat gattgctcag gggaacccga tgccggtgcc ttacggtgaa
      121 atgctggttg gttcacgacg gatatcccag gacatcagca cccgtgatga gggcgggggc
      181 ggaaaggtcg tggttatcgg gcggcagagg taaaaagaat aaaaaaatcc cgcagtgtat
      241 ggaggctgcg ggaacagaaa atgaagatta accacaggga gttttgtttt tattggcccg
      301 aaaaaactgt aacgcccggg aatgatatct gccac
//
LOCUS       DAZKHF020000855          334 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2201S31241
            SAMN43267800-rid25106413.denovo.862, whole genome shotgun sequence.
ACCESSION   DAZKHF020000855 DAZKHF020000000
VERSION     DAZKHF020000855.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267800
            Sequence Read Archive: SRR30307263
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 334)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 334)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 334)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350581.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350581.2
            Genome Coverage       :: 151x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:21:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,652
            CDSs (total)                      :: 5,557
            Genes (coding)                    :: 5,320
            CDSs (with protein)               :: 5,320
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 237
            CDSs (without protein)            :: 237
            Pseudo Genes (ambiguous residues) :: 0 of 237
            Pseudo Genes (frameshifted)       :: 102 of 237
            Pseudo Genes (incomplete)         :: 130 of 237
            Pseudo Genes (internal stop)      :: 52 of 237
            Pseudo Genes (multiple problems)  :: 41 of 237
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..334
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267800-rid25106413.denovo.862"
                     /serotype="O 26:H11"
                     /isolate="2201S31241"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-01"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            <1..>334
                     /gene="cesT"
                     /locus_tag="ACHOSC_RS27865"
                     /old_locus_tag="pgaptmp_005573"
     CDS             <1..>334
                     /gene="cesT"
                     /locus_tag="ACHOSC_RS27865"
                     /old_locus_tag="pgaptmp_005573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="type III secretion system LEE chaperone CesT"
                     /protein_id="HGU4551260.1"
                     /translation="LSDANDEYMMIYGVCGKFPTDNPNFALEILNANLWFAENGGPYL
                     CYESGAQSLLLALRFPLDDATPEKLENEIEVVVKSMENLYLVLHNQGITLENEHMKIE
                     EISSSDNKH"
BASE COUNT          113 a           52 c           72 g           97 t
ORIGIN      
        1 gttatctgat gctaatgacg aatatatgat gatctatggc gtctgtggga aattcccgac
       61 agataatcct aacttcgctc ttgagatttt gaatgcaaac ttatggtttg cagagaatgg
      121 tggaccatac ctgtgttatg aatcaggagc acaatcgctg ttgttagcgt tacgttttcc
      181 gctcgatgat gctacacctg aaaaacttga gaatgaaata gaagtcgtcg ttaagtcaat
      241 ggaaaacctg tatttggtat tacataatca gggaataaca ttagaaaacg aacatatgaa
      301 aatagaggaa attagttcaa gcgataataa acat
//
LOCUS       DAZKHF020000856          330 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2201S31241
            SAMN43267800-rid25106413.denovo.863, whole genome shotgun sequence.
ACCESSION   DAZKHF020000856 DAZKHF020000000
VERSION     DAZKHF020000856.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267800
            Sequence Read Archive: SRR30307263
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 330)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 330)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 330)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350581.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350581.2
            Genome Coverage       :: 151x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:21:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,652
            CDSs (total)                      :: 5,557
            Genes (coding)                    :: 5,320
            CDSs (with protein)               :: 5,320
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 237
            CDSs (without protein)            :: 237
            Pseudo Genes (ambiguous residues) :: 0 of 237
            Pseudo Genes (frameshifted)       :: 102 of 237
            Pseudo Genes (incomplete)         :: 130 of 237
            Pseudo Genes (internal stop)      :: 52 of 237
            Pseudo Genes (multiple problems)  :: 41 of 237
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..330
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267800-rid25106413.denovo.863"
                     /serotype="O 26:H11"
                     /isolate="2201S31241"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-01"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            <1..>330
                     /locus_tag="ACHOSC_RS27870"
                     /old_locus_tag="pgaptmp_005574"
     CDS             <1..>330
                     /locus_tag="ACHOSC_RS27870"
                     /old_locus_tag="pgaptmp_005574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309619.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HGU4551261.1"
                     /translation="YLPWEVLHLRYSTDDGFMGRSPVTICRESLGLGLAQQRHGASVM
                     RDGMMAAGVITSGEWLDGVKGKQALAALERYKGARNAGKTPILEGGMSYQQLGMSNQD
                     AEWLASRR"
BASE COUNT           72 a           86 c          109 g           63 t
ORIGIN      
        1 tatttaccgt gggaggttct ccaccttcgt tactccacgg atgacggttt tatggggcgc
       61 tcacctgtaa ccatctgccg cgaatcgctg gggcttgggc tggcccaaca acgccacggc
      121 gcgagcgtga tgcgtgatgg catgatggcg gcaggggtta tcacgtcagg cgaatggctg
      181 gacggcgtga agggcaaaca ggcattagcc gcactggaac gctacaaagg ggccagaaac
      241 gccggaaaaa cgcccatcct tgaagggggt atgagctatc agcagctggg catgagtaat
      301 caggatgctg aatggctggc ctcccgtcgc
//
LOCUS       DAZKHF020000857          325 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2201S31241
            SAMN43267800-rid25106413.denovo.864, whole genome shotgun sequence.
ACCESSION   DAZKHF020000857 DAZKHF020000000
VERSION     DAZKHF020000857.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267800
            Sequence Read Archive: SRR30307263
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 325)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 325)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 325)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350581.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350581.2
            Genome Coverage       :: 151x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:21:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,652
            CDSs (total)                      :: 5,557
            Genes (coding)                    :: 5,320
            CDSs (with protein)               :: 5,320
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 237
            CDSs (without protein)            :: 237
            Pseudo Genes (ambiguous residues) :: 0 of 237
            Pseudo Genes (frameshifted)       :: 102 of 237
            Pseudo Genes (incomplete)         :: 130 of 237
            Pseudo Genes (internal stop)      :: 52 of 237
            Pseudo Genes (multiple problems)  :: 41 of 237
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..325
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267800-rid25106413.denovo.864"
                     /serotype="O 26:H11"
                     /isolate="2201S31241"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-01"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            <1..>325
                     /locus_tag="ACHOSC_RS27875"
                     /old_locus_tag="pgaptmp_005575"
     CDS             <1..>325
                     /locus_tag="ACHOSC_RS27875"
                     /old_locus_tag="pgaptmp_005575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF968 domain-containing protein"
                     /protein_id="HGU4551262.1"
                     /translation="IIPGEQTATSILKQRTKNIALPPHTHQQQNPPQEKTVVSIAVDP
                     ESPESFMKRPKRRRWVNEKYTRWVKTQPCACCGKPADDPHHLIGHGQGGMGTKSHDIF
                     TLPLCR"
BASE COUNT           94 a           90 c           82 g           59 t
ORIGIN      
        1 atcataccgg gagaacagac cgccaccagc atactgaagc agcgcacaaa aaatattgcg
       61 ctaccgcctc acacccacca gcaacagaac ccaccacagg aaaagacggt ggtcagcatt
      121 gccgttgatc cggagtctcc ggaatccttc atgaaacgac ctaaacgtcg ccgctgggta
      181 aatgagaaat acacacgctg ggtaaagaca cagccgtgtg cgtgttgtgg taagccagcg
      241 gacgatcctc atcatctgat tggtcatggt cagggtggaa tgggaacaaa atcccacgat
      301 attttcacgc taccgctgtg tcggg
//
LOCUS       DAZKHF020000858          320 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2201S31241
            SAMN43267800-rid25106413.denovo.865, whole genome shotgun sequence.
ACCESSION   DAZKHF020000858 DAZKHF020000000
VERSION     DAZKHF020000858.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267800
            Sequence Read Archive: SRR30307263
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 320)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 320)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 320)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350581.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350581.2
            Genome Coverage       :: 151x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:21:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,652
            CDSs (total)                      :: 5,557
            Genes (coding)                    :: 5,320
            CDSs (with protein)               :: 5,320
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 237
            CDSs (without protein)            :: 237
            Pseudo Genes (ambiguous residues) :: 0 of 237
            Pseudo Genes (frameshifted)       :: 102 of 237
            Pseudo Genes (incomplete)         :: 130 of 237
            Pseudo Genes (internal stop)      :: 52 of 237
            Pseudo Genes (multiple problems)  :: 41 of 237
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..320
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267800-rid25106413.denovo.865"
                     /serotype="O 26:H11"
                     /isolate="2201S31241"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-01"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            85..>320
                     /gene="grlR"
                     /locus_tag="ACHOSC_RS27880"
                     /old_locus_tag="pgaptmp_005576"
     CDS             85..>320
                     /gene="grlR"
                     /locus_tag="ACHOSC_RS27880"
                     /old_locus_tag="pgaptmp_005576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system LEE GrlA-binding
                     negative regulator GrlR"
                     /protein_id="HGU4551263.1"
                     /translation="MIMKDGIYSIIFISNEDSCGEGILIKNGNLITGGDIASVYQGVL
                     SEEEDIILHVHRYNHEIPSVLNIEQDYQLVIPKKV"
BASE COUNT          104 a           45 c           73 g           98 t
ORIGIN      
        1 aaagaaaggt ctccgttatt cttgatatcg cttatggata gaacaaattg aaaggagtga
       61 ggttagtatg aaactgagtg agttatgatt atgaaggatg gcatctatag cattatcttt
      121 attagcaatg aagattcctg tggggaaggt atactgatta aaaatggaaa tttgatcact
      181 ggcggagata ttgcttctgt gtatcagggg gtcctgtctg aagaggagga catcatactt
      241 catgtccatc gctataatca tgaaattccc tcggtgctaa acattgaaca agattatcaa
      301 ttagttatcc ctaaaaaagt
//
LOCUS       DAZKHF020000859          320 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2201S31241
            SAMN43267800-rid25106413.denovo.866, whole genome shotgun sequence.
ACCESSION   DAZKHF020000859 DAZKHF020000000
VERSION     DAZKHF020000859.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267800
            Sequence Read Archive: SRR30307263
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 320)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 320)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 320)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350581.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350581.2
            Genome Coverage       :: 151x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:21:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,652
            CDSs (total)                      :: 5,557
            Genes (coding)                    :: 5,320
            CDSs (with protein)               :: 5,320
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 237
            CDSs (without protein)            :: 237
            Pseudo Genes (ambiguous residues) :: 0 of 237
            Pseudo Genes (frameshifted)       :: 102 of 237
            Pseudo Genes (incomplete)         :: 130 of 237
            Pseudo Genes (internal stop)      :: 52 of 237
            Pseudo Genes (multiple problems)  :: 41 of 237
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..320
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267800-rid25106413.denovo.866"
                     /serotype="O 26:H11"
                     /isolate="2201S31241"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-01"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            <1..>320
                     /locus_tag="ACHOSC_RS27885"
                     /old_locus_tag="pgaptmp_005577"
     CDS             <1..>320
                     /locus_tag="ACHOSC_RS27885"
                     /old_locus_tag="pgaptmp_005577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4551264.1"
                     /translation="QQKEQLALAGQNTELAKLKYQTAQGELKTLTEMQKQELLRNAAL
                     IDQQKIREQLRSREETLKNDNVAARASNEAELLGYGQGERARERMRELQQIRDSFRQK
                     DADL"
BASE COUNT           95 a           77 c          106 g           42 t
ORIGIN      
        1 agcagcagaa agagcaactg gcactggcgg gtcagaatac agagctggcg aagctgaagt
       61 accagactgc gcagggcgaa ctgaaaaccc tgacggagat gcagaagcag gaactgctgc
      121 gtaacgcggc cctgattgac cagcaaaaaa tccgggaaca gttgcgatcc cgggaagaga
      181 ccctgaagaa tgataatgtg gctgcgcgtg catcaaatga agccgaactg ctgggatacg
      241 ggcagggaga acgagccagg gaacgcatgc gggagttgca gcagatccgc gacagcttcc
      301 gccagaagga tgcggacctt
//
LOCUS       DAZKHF020000860          319 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2201S31241
            SAMN43267800-rid25106413.denovo.867, whole genome shotgun sequence.
ACCESSION   DAZKHF020000860 DAZKHF020000000
VERSION     DAZKHF020000860.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267800
            Sequence Read Archive: SRR30307263
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 319)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 319)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 319)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350581.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350581.2
            Genome Coverage       :: 151x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:21:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,652
            CDSs (total)                      :: 5,557
            Genes (coding)                    :: 5,320
            CDSs (with protein)               :: 5,320
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 237
            CDSs (without protein)            :: 237
            Pseudo Genes (ambiguous residues) :: 0 of 237
            Pseudo Genes (frameshifted)       :: 102 of 237
            Pseudo Genes (incomplete)         :: 130 of 237
            Pseudo Genes (internal stop)      :: 52 of 237
            Pseudo Genes (multiple problems)  :: 41 of 237
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..319
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267800-rid25106413.denovo.867"
                     /serotype="O 26:H11"
                     /isolate="2201S31241"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-01"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            <1..308
                     /locus_tag="ACHOSC_RS27890"
                     /old_locus_tag="pgaptmp_005578"
     CDS             <1..308
                     /locus_tag="ACHOSC_RS27890"
                     /old_locus_tag="pgaptmp_005578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012816780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004842 - ubiquitin-protein transferase
                     activity [Evidence IEA];
                     GO_process: GO:0044403 - biological process involved in
                     symbiotic interaction [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="T3SS effector NleG family protein"
                     /protein_id="HGU4551265.1"
                     /translation="NKILMEKINSCVFRPDSNHFSCPESFLTCPITLDTPENGVFMRN
                     SRGAEICSLYDKDALVQLVETGGAHPLSREPITESMIMRKDECHFDSKKEAFVASDA"
BASE COUNT          101 a           54 c           67 g           97 t
ORIGIN      
        1 gtaataaaat attaatggaa aaaataaatt cttgtgtatt tagaccggat tctaatcact
       61 tttcttgccc ggagtcattt ttgacatgcc cgataacgct ggacacacct gagaatggag
      121 tgttcatgag aaactcacga ggtgctgaga tatgctctct atatgataag gacgcgttag
      181 tgcaacttgt tgaaactggt ggagctcatc ctctgagtcg agaacctata acagaatcaa
      241 tgatcatgag aaaagacgaa tgtcactttg attcaaaaaa agaagccttt gttgcaagtg
      301 atgcttaatt ttttctgtt
//
LOCUS       DAZKHF020000861          316 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2201S31241
            SAMN43267800-rid25106413.denovo.868, whole genome shotgun sequence.
ACCESSION   DAZKHF020000861 DAZKHF020000000
VERSION     DAZKHF020000861.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267800
            Sequence Read Archive: SRR30307263
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 316)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 316)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 316)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350581.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350581.2
            Genome Coverage       :: 151x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:21:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,652
            CDSs (total)                      :: 5,557
            Genes (coding)                    :: 5,320
            CDSs (with protein)               :: 5,320
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 237
            CDSs (without protein)            :: 237
            Pseudo Genes (ambiguous residues) :: 0 of 237
            Pseudo Genes (frameshifted)       :: 102 of 237
            Pseudo Genes (incomplete)         :: 130 of 237
            Pseudo Genes (internal stop)      :: 52 of 237
            Pseudo Genes (multiple problems)  :: 41 of 237
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..316
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267800-rid25106413.denovo.868"
                     /serotype="O 26:H11"
                     /isolate="2201S31241"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-01"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            <1..266
                     /locus_tag="ACHOSC_RS27895"
                     /old_locus_tag="pgaptmp_005579"
     CDS             <1..266
                     /locus_tag="ACHOSC_RS27895"
                     /old_locus_tag="pgaptmp_005579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016245103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="OB-fold-containig protein"
                     /protein_id="HGU4551266.1"
                     /translation="APWLPRDETTALTEEAFIGCMATITGYHAVAGSPCEGRFTDRFG
                     QVHYLLLEPEAGKEFRRGDKVIIVCRLSGSRWLGELNPWPDVL"
BASE COUNT           62 a           84 c           90 g           80 t
ORIGIN      
        1 ttgccccctg gttaccccgt gatgaaacaa cggcgctcac cgaggaggca ttcatcgggt
       61 gtatggccac catcaccggt tatcacgcag tggcgggttc cccctgtgaa ggtcgcttca
      121 cagaccgctt tgggcaggtt cattacctgt tgctggagcc ggaggccggg aaagaattca
      181 gacgaggaga caaagtgatt attgtctgcc gtctttccgg ttcccgctgg ctgggcgaac
      241 tgaatccctg gccggatgtt ctttaatccc gacatataca gaggttctca ctatgtttaa
      301 tcttccggga tggttg
//
LOCUS       DAZKHF020000862          314 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2201S31241
            SAMN43267800-rid25106413.denovo.869, whole genome shotgun sequence.
ACCESSION   DAZKHF020000862 DAZKHF020000000
VERSION     DAZKHF020000862.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267800
            Sequence Read Archive: SRR30307263
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 314)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 314)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 314)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350581.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350581.2
            Genome Coverage       :: 151x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:21:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,652
            CDSs (total)                      :: 5,557
            Genes (coding)                    :: 5,320
            CDSs (with protein)               :: 5,320
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 237
            CDSs (without protein)            :: 237
            Pseudo Genes (ambiguous residues) :: 0 of 237
            Pseudo Genes (frameshifted)       :: 102 of 237
            Pseudo Genes (incomplete)         :: 130 of 237
            Pseudo Genes (internal stop)      :: 52 of 237
            Pseudo Genes (multiple problems)  :: 41 of 237
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..314
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267800-rid25106413.denovo.869"
                     /serotype="O 26:H11"
                     /isolate="2201S31241"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-01"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            <1..>314
                     /locus_tag="ACHOSC_RS27900"
                     /old_locus_tag="pgaptmp_005580"
     CDS             <1..>314
                     /locus_tag="ACHOSC_RS27900"
                     /old_locus_tag="pgaptmp_005580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EF-Tu/IF-2/RF-3 family GTPase"
                     /protein_id="HGU4551267.1"
                     /translation="IDKPFLLPIEDVFSISGRGTVVTGRVERGIIKVGEEVEIVGIKE
                     TQKSTCTGVEMFRKLLDEGRAGENVGVLLRGIKREEIERGQVLAKPGTIKPHTKFESE
                     VY"
BASE COUNT           80 a           70 c           88 g           76 t
ORIGIN      
        1 attgacaagc cgttcctgct gccgatcgaa gacgtattct ccatctccgg tcgtggtacc
       61 gttgttaccg gtcgtgtaga acgcggtatc atcaaagttg gtgaagaagt tgaaatcgtt
      121 ggtatcaaag agactcagaa gtctacctgt actggcgttg aaatgttccg caaactgctg
      181 gacgaaggcc gtgctggtga gaacgtaggt gttctgctgc gtggtatcaa acgtgaagaa
      241 atcgaacgtg gtcaggtact ggctaagccg ggcaccatca agccgcacac caagttcgaa
      301 tctgaagtgt acat
//
LOCUS       DAZKHF020000863          305 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2201S31241
            SAMN43267800-rid25106413.denovo.870, whole genome shotgun sequence.
ACCESSION   DAZKHF020000863 DAZKHF020000000
VERSION     DAZKHF020000863.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267800
            Sequence Read Archive: SRR30307263
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 305)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 305)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 305)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350581.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350581.2
            Genome Coverage       :: 151x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:21:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,652
            CDSs (total)                      :: 5,557
            Genes (coding)                    :: 5,320
            CDSs (with protein)               :: 5,320
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 237
            CDSs (without protein)            :: 237
            Pseudo Genes (ambiguous residues) :: 0 of 237
            Pseudo Genes (frameshifted)       :: 102 of 237
            Pseudo Genes (incomplete)         :: 130 of 237
            Pseudo Genes (internal stop)      :: 52 of 237
            Pseudo Genes (multiple problems)  :: 41 of 237
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..305
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267800-rid25106413.denovo.870"
                     /serotype="O 26:H11"
                     /isolate="2201S31241"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-01"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            <1..235
                     /gene="ybiJ"
                     /locus_tag="ACHOSC_RS27905"
                     /old_locus_tag="pgaptmp_005581"
     CDS             <1..235
                     /gene="ybiJ"
                     /locus_tag="ACHOSC_RS27905"
                     /old_locus_tag="pgaptmp_005581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF1471 family protein YbiJ"
                     /protein_id="HGU4551268.1"
                     /translation="AMALSTLSFGVFAAEPVTASQAQNMNKIGVVSADGASTLDALEA
                     KLAEKAAAAGASGYSITSATNNNKLSGTAVIYK"
BASE COUNT           76 a           83 c           80 g           66 t
ORIGIN      
        1 tgctatggct ctttcaactc tgtcatttgg cgtgtttgcc gcggaaccgg taacggcatc
       61 ccaggcacag aacatgaata aaatcggcgt ggtttctgcc gatggcgcat ccaccctcga
      121 tgccctggaa gcgaaactgg ctgagaaagc cgcggcagcc ggtgccagtg gatacagcat
      181 cacttccgcc accaacaaca ataaattaag cggtactgcg gtaatttata agtaattcgt
      241 aggacggata aggcgtttac gccgcatccg gcaacagatg cctgatgcga cgctaccgcg
      301 tctta
//
LOCUS       DAZKHF020000864          303 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2201S31241
            SAMN43267800-rid25106413.denovo.871, whole genome shotgun sequence.
ACCESSION   DAZKHF020000864 DAZKHF020000000
VERSION     DAZKHF020000864.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267800
            Sequence Read Archive: SRR30307263
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 303)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 303)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 303)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350581.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350581.2
            Genome Coverage       :: 151x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:21:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,652
            CDSs (total)                      :: 5,557
            Genes (coding)                    :: 5,320
            CDSs (with protein)               :: 5,320
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 237
            CDSs (without protein)            :: 237
            Pseudo Genes (ambiguous residues) :: 0 of 237
            Pseudo Genes (frameshifted)       :: 102 of 237
            Pseudo Genes (incomplete)         :: 130 of 237
            Pseudo Genes (internal stop)      :: 52 of 237
            Pseudo Genes (multiple problems)  :: 41 of 237
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..303
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267800-rid25106413.denovo.871"
                     /serotype="O 26:H11"
                     /isolate="2201S31241"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-01"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            54..>303
                     /locus_tag="ACHOSC_RS27910"
                     /old_locus_tag="pgaptmp_005582"
     CDS             54..>303
                     /locus_tag="ACHOSC_RS27910"
                     /old_locus_tag="pgaptmp_005582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003796 - lysozyme activity [Evidence
                     IEA];
                     GO_process: GO:0009253 - peptidoglycan catabolic process
                     [Evidence IEA];
                     GO_process: GO:0016998 - cell wall macromolecule catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family protein"
                     /protein_id="HGU4551269.1"
                     /translation="MSPSLRKAVAAAIGGGAVAIASVLITGPGGNDGLEGVSYIPYKD
                     IVGVWTVCHGHTGKDIMPGKTYTEAECKALLNKDLATVA"
BASE COUNT           85 a           66 c           81 g           71 t
ORIGIN      
        1 aatctttatt tcaagattaa agaagataag cgtaaggctg cgagaggtga ataatgtcgc
       61 cgtcattacg caaggctgtt gcagctgcta ttggtggtgg ggctgttgcc atagcgtctg
      121 tgctcatcac tggtccaggt ggtaacgatg gtctggaagg tgtcagctac ataccataca
      181 aagatatcgt tggcgtatgg actgtatgtc acggacacac cggaaaagac atcatgcccg
      241 gtaaaacgta taccgaagca gaatgcaaag ccctcctgaa taaagacctt gccacggtcg
      301 cca
//
LOCUS       DAZKHF020000865          302 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2201S31241
            SAMN43267800-rid25106413.denovo.872, whole genome shotgun sequence.
ACCESSION   DAZKHF020000865 DAZKHF020000000
VERSION     DAZKHF020000865.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267800
            Sequence Read Archive: SRR30307263
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 302)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 302)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 302)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350581.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350581.2
            Genome Coverage       :: 151x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:21:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,652
            CDSs (total)                      :: 5,557
            Genes (coding)                    :: 5,320
            CDSs (with protein)               :: 5,320
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 237
            CDSs (without protein)            :: 237
            Pseudo Genes (ambiguous residues) :: 0 of 237
            Pseudo Genes (frameshifted)       :: 102 of 237
            Pseudo Genes (incomplete)         :: 130 of 237
            Pseudo Genes (internal stop)      :: 52 of 237
            Pseudo Genes (multiple problems)  :: 41 of 237
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..302
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267800-rid25106413.denovo.872"
                     /serotype="O 26:H11"
                     /isolate="2201S31241"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-01"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            2..>302
                     /locus_tag="ACHOSC_RS27915"
                     /old_locus_tag="pgaptmp_005583"
     CDS             2..>302
                     /locus_tag="ACHOSC_RS27915"
                     /old_locus_tag="pgaptmp_005583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001365813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PrgH/EprH family type III secretion apparatus
                     protein"
                     /protein_id="HGU4551270.1"
                     /translation="MLSGPLNGCEYEILNGRLLVIIGNDVSLGRSDAFSELPENTIVV
                     PYGELTGSFEIIITTDPDLVVTFRELTAQKPEDRTLTLNQQIEVLGLKFAVKEKNE"
BASE COUNT          104 a           43 c           65 g           90 t
ORIGIN      
        1 tttgttgagc ggaccattga atggatgcga gtatgaaata cttaatgggc gtctacttgt
       61 tatcattggt aatgatgttt cgttaggaag aagtgatgct ttttctgagt taccagaaaa
      121 cacaatagtc gttccttatg gcgagcttac aggtagtttt gagataataa ttactaccga
      181 ccctgatctg gtagtaacat tcagagaatt aacagcacaa aaacctgaag atagaacgtt
      241 aacattgaat cagcaaatag aagttttagg tctcaaattt gctgtaaaag aaaaaaatga
      301 ag
//
LOCUS       DAZKHF020000866          301 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2201S31241
            SAMN43267800-rid25106413.denovo.873, whole genome shotgun sequence.
ACCESSION   DAZKHF020000866 DAZKHF020000000
VERSION     DAZKHF020000866.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267800
            Sequence Read Archive: SRR30307263
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 301)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 301)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 301)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350581.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350581.2
            Genome Coverage       :: 151x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:21:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,652
            CDSs (total)                      :: 5,557
            Genes (coding)                    :: 5,320
            CDSs (with protein)               :: 5,320
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 237
            CDSs (without protein)            :: 237
            Pseudo Genes (ambiguous residues) :: 0 of 237
            Pseudo Genes (frameshifted)       :: 102 of 237
            Pseudo Genes (incomplete)         :: 130 of 237
            Pseudo Genes (internal stop)      :: 52 of 237
            Pseudo Genes (multiple problems)  :: 41 of 237
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..301
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267800-rid25106413.denovo.873"
                     /serotype="O 26:H11"
                     /isolate="2201S31241"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-01"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
BASE COUNT           90 a           52 c           66 g           93 t
ORIGIN      
        1 aggcctccgc aagtgttcgt ttggcttcac atggcatctt cttcttagaa aaatatcgac
       61 atattttgtg acacgaattg caaatctggt tttgttgtat ggattgcgtg atttttgatc
      121 tggtataaca ggtataaagg tgcaccaaga tagtcaatga gacagggcat ctcgcaatct
      181 atggcaaaca tcacttcagt tctttcacat cgggtgatga aaatgcactt cagtctgaaa
      241 ggaatatgaa aatgagatca acagacactc tattttatga ctctgggtaa aatggattga
      301 g
//
LOCUS       DAZKHF020000867          298 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2201S31241
            SAMN43267800-rid25106413.denovo.874, whole genome shotgun sequence.
ACCESSION   DAZKHF020000867 DAZKHF020000000
VERSION     DAZKHF020000867.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267800
            Sequence Read Archive: SRR30307263
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 298)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 298)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 298)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350581.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350581.2
            Genome Coverage       :: 151x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:21:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,652
            CDSs (total)                      :: 5,557
            Genes (coding)                    :: 5,320
            CDSs (with protein)               :: 5,320
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 237
            CDSs (without protein)            :: 237
            Pseudo Genes (ambiguous residues) :: 0 of 237
            Pseudo Genes (frameshifted)       :: 102 of 237
            Pseudo Genes (incomplete)         :: 130 of 237
            Pseudo Genes (internal stop)      :: 52 of 237
            Pseudo Genes (multiple problems)  :: 41 of 237
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..298
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267800-rid25106413.denovo.874"
                     /serotype="O 26:H11"
                     /isolate="2201S31241"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-01"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            <1..297
                     /locus_tag="ACHOSC_RS27920"
                     /old_locus_tag="pgaptmp_005584"
     CDS             <1..297
                     /locus_tag="ACHOSC_RS27920"
                     /old_locus_tag="pgaptmp_005584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000833619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4551271.1"
                     /translation="ELFTDADGELITSLVVVDKPRPPVELERIEEAGNKTENHAALWQ
                     CIRTRTAHGEACTIALLRDDMKKLGYEMKHFRRWLYKLENDGVIVIDGDDVRPL"
BASE COUNT           73 a           72 c           97 g           56 t
ORIGIN      
        1 gagcttttta ccgacgctga cggggagtta atcacgtcgc tggtggtggt ggataagccg
       61 cgccctcccg ttgaactgga gcgcatcgag gaggcaggga acaagacgga gaatcacgcc
      121 gcgctatggc agtgcatcag aacacgaaca gcacacgggg aggcgtgcac tatcgccctt
      181 ctccgcgacg atatgaaaaa gctggggtat gagatgaagc acttccggcg ctggctgtac
      241 aagctggaaa atgatggcgt aatcgttatt gacggtgatg acgtgcgccc gctgtaaa
//
LOCUS       DAZKHF020000868          295 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2201S31241
            SAMN43267800-rid25106413.denovo.875, whole genome shotgun sequence.
ACCESSION   DAZKHF020000868 DAZKHF020000000
VERSION     DAZKHF020000868.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267800
            Sequence Read Archive: SRR30307263
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 295)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 295)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 295)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350581.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350581.2
            Genome Coverage       :: 151x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:21:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,652
            CDSs (total)                      :: 5,557
            Genes (coding)                    :: 5,320
            CDSs (with protein)               :: 5,320
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 237
            CDSs (without protein)            :: 237
            Pseudo Genes (ambiguous residues) :: 0 of 237
            Pseudo Genes (frameshifted)       :: 102 of 237
            Pseudo Genes (incomplete)         :: 130 of 237
            Pseudo Genes (internal stop)      :: 52 of 237
            Pseudo Genes (multiple problems)  :: 41 of 237
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..295
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267800-rid25106413.denovo.875"
                     /serotype="O 26:H11"
                     /isolate="2201S31241"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-01"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            complement(<1..54)
                     /locus_tag="ACHOSC_RS27925"
                     /old_locus_tag="pgaptmp_005585"
     CDS             complement(<1..54)
                     /locus_tag="ACHOSC_RS27925"
                     /old_locus_tag="pgaptmp_005585"
                     /inference="COORDINATES: protein motif:HMM:NF017845.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PerC family transcriptional regulator"
                     /protein_id="HGU4551272.1"
                     /translation="MLHDHVAECLEKKGLYRR"
BASE COUNT          120 a           69 c           32 g           74 t
ORIGIN      
        1 tctccggtac agtccttttt tctccagaca ttctgccacg tgatcatgta gcataagtga
       61 cctccgatta tctacagact gccatcctga atttaccttc ccttaatgaa ataacaataa
      121 aaaacaaacc acgcaaaaac aataaaacaa cacacaaaaa aaactaaata ataaacaaaa
      181 ataatcacct tattttatta ttttttgagg gagcaattac tgaacaaaaa acgctgacta
      241 tatactcaaa accaaacaac tattctgcca atcaggtatc atggcaacac acgga
//
LOCUS       DAZKHF020000869          293 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2201S31241
            SAMN43267800-rid25106413.denovo.876, whole genome shotgun sequence.
ACCESSION   DAZKHF020000869 DAZKHF020000000
VERSION     DAZKHF020000869.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267800
            Sequence Read Archive: SRR30307263
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 293)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 293)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 293)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350581.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350581.2
            Genome Coverage       :: 151x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:21:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,652
            CDSs (total)                      :: 5,557
            Genes (coding)                    :: 5,320
            CDSs (with protein)               :: 5,320
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 237
            CDSs (without protein)            :: 237
            Pseudo Genes (ambiguous residues) :: 0 of 237
            Pseudo Genes (frameshifted)       :: 102 of 237
            Pseudo Genes (incomplete)         :: 130 of 237
            Pseudo Genes (internal stop)      :: 52 of 237
            Pseudo Genes (multiple problems)  :: 41 of 237
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..293
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267800-rid25106413.denovo.876"
                     /serotype="O 26:H11"
                     /isolate="2201S31241"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-01"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            <1..>293
                     /locus_tag="ACHOSC_RS27930"
                     /old_locus_tag="pgaptmp_005586"
     CDS             <1..>293
                     /locus_tag="ACHOSC_RS27930"
                     /old_locus_tag="pgaptmp_005586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6645 domain-containing protein"
                     /protein_id="HGU4551273.1"
                     /translation="LGIYLYTDAPVPDGVAMTGTGNPFLMSYFTQTTDGRVNLMHHRK
                     AGNTKLGEFGDYGNDWQTLELVFTAGSATVTPKLNGVAGPAFQVIKDSLTLGL"
BASE COUNT           68 a           70 c           91 g           64 t
ORIGIN      
        1 ctggggattt atctgtatac ggatgctccc gttcctgatg gtgtggcgat gacgggtacc
       61 ggtaatccgt tcctgatgtc gtacttcact cagaccactg acggcagagt gaatctgatg
      121 catcacagga aagccggaaa cacgaagctg ggggagttcg gcgattacgg taacgactgg
      181 cagacgctgg agctggtgtt caccgccggc agtgccacgg ttactccgaa actgaatgga
      241 gtggctggcc cggcattcca ggttataaaa gacagtctga cactgggact gaa
//
LOCUS       DAZKHF020000870          291 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2201S31241
            SAMN43267800-rid25106413.denovo.877, whole genome shotgun sequence.
ACCESSION   DAZKHF020000870 DAZKHF020000000
VERSION     DAZKHF020000870.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267800
            Sequence Read Archive: SRR30307263
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 291)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 291)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 291)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350581.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350581.2
            Genome Coverage       :: 151x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:21:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,652
            CDSs (total)                      :: 5,557
            Genes (coding)                    :: 5,320
            CDSs (with protein)               :: 5,320
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 237
            CDSs (without protein)            :: 237
            Pseudo Genes (ambiguous residues) :: 0 of 237
            Pseudo Genes (frameshifted)       :: 102 of 237
            Pseudo Genes (incomplete)         :: 130 of 237
            Pseudo Genes (internal stop)      :: 52 of 237
            Pseudo Genes (multiple problems)  :: 41 of 237
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..291
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267800-rid25106413.denovo.877"
                     /serotype="O 26:H11"
                     /isolate="2201S31241"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-01"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            complement(<1..>291)
                     /locus_tag="ACHOSC_RS27935"
                     /old_locus_tag="pgaptmp_005587"
     CDS             complement(<1..>291)
                     /locus_tag="ACHOSC_RS27935"
                     /old_locus_tag="pgaptmp_005587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HGU4551274.1"
                     /translation="WLVCRGEIHKFRCVPHLTGRRFEHGVTDCYTLFRDAYHLAEIEM
                     PDFVREDDWWRNGQNLYLDNLEATGLYQVPLSAAQPGDVLLCCFGSSVPNHAA"
BASE COUNT           77 a          100 c           68 g           46 t
ORIGIN      
        1 agcggcgtga ttcggcaccg atgaaccaaa acagcacagc agcacatcgc ccggctgcgc
       61 cgctgacaac ggcacctgat acagccctgt ggcctccaga ttatccagat agagattctg
      121 accgttacgc caccagtcat cctcacgcac aaaatccggc atctcaatct ccgccagatg
      181 ataagcatcc cggaacagtg tgtaacagtc cgtcacaccg tgctcaaagc gccgcccggt
      241 gagatgcggc acacagcgga acttatgaat ctcaccccgg cagaccagcc a
//
LOCUS       DAZKHF020000871          290 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2201S31241
            SAMN43267800-rid25106413.denovo.878, whole genome shotgun sequence.
ACCESSION   DAZKHF020000871 DAZKHF020000000
VERSION     DAZKHF020000871.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267800
            Sequence Read Archive: SRR30307263
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 290)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 290)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 290)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350581.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350581.2
            Genome Coverage       :: 151x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:21:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,652
            CDSs (total)                      :: 5,557
            Genes (coding)                    :: 5,320
            CDSs (with protein)               :: 5,320
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 237
            CDSs (without protein)            :: 237
            Pseudo Genes (ambiguous residues) :: 0 of 237
            Pseudo Genes (frameshifted)       :: 102 of 237
            Pseudo Genes (incomplete)         :: 130 of 237
            Pseudo Genes (internal stop)      :: 52 of 237
            Pseudo Genes (multiple problems)  :: 41 of 237
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..290
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267800-rid25106413.denovo.878"
                     /serotype="O 26:H11"
                     /isolate="2201S31241"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-01"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            <1..240
                     /locus_tag="ACHOSC_RS27940"
                     /old_locus_tag="pgaptmp_005588"
     CDS             <1..240
                     /locus_tag="ACHOSC_RS27940"
                     /old_locus_tag="pgaptmp_005588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001111277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage anti-RecBCD protein"
                     /protein_id="HGU4551275.1"
                     /translation="VSEVLDKFRKNYDRIMSLPQETKEEKEFRHCIWLAEKEERERIY
                     QTSIRPFRKATYTHFPEIDPRLRNYRSRYGAISND"
BASE COUNT           92 a           71 c           66 g           61 t
ORIGIN      
        1 gtatcggagg tgctggataa attcagaaaa aactacgatc ggataatgtc gctaccgcag
       61 gaaacgaaag aggaaaagga atttcgccat tgtatatggc ttgcagagaa agaagaacgc
      121 gagcgaattt accagacatc aatccgacca ttccgcaaag ccacatatac ccacttccct
      181 gaaattgacc cgcgcctgcg taattaccgc tcacgctatg gcgctatcag taatgactga
      241 ggaatttacc atgagaggac ttgcatacaa tcccggcatt cttccggcag
//
LOCUS       DAZKHF020000872          289 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2201S31241
            SAMN43267800-rid25106413.denovo.879, whole genome shotgun sequence.
ACCESSION   DAZKHF020000872 DAZKHF020000000
VERSION     DAZKHF020000872.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267800
            Sequence Read Archive: SRR30307263
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 289)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 289)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 289)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350581.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350581.2
            Genome Coverage       :: 151x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:21:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,652
            CDSs (total)                      :: 5,557
            Genes (coding)                    :: 5,320
            CDSs (with protein)               :: 5,320
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 237
            CDSs (without protein)            :: 237
            Pseudo Genes (ambiguous residues) :: 0 of 237
            Pseudo Genes (frameshifted)       :: 102 of 237
            Pseudo Genes (incomplete)         :: 130 of 237
            Pseudo Genes (internal stop)      :: 52 of 237
            Pseudo Genes (multiple problems)  :: 41 of 237
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..289
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267800-rid25106413.denovo.879"
                     /serotype="O 26:H11"
                     /isolate="2201S31241"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-01"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            6..>289
                     /gene="gpFI"
                     /locus_tag="ACHOSC_RS27945"
                     /old_locus_tag="pgaptmp_005589"
     CDS             6..>289
                     /gene="gpFI"
                     /locus_tag="ACHOSC_RS27945"
                     /old_locus_tag="pgaptmp_005589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-packaging protein FI"
                     /protein_id="HGU4551276.1"
                     /translation="MTKDELIARLRSLGEQLNRDVSLTGTKEELALRVAELEEELDDT
                     DDAAGQDTSVSPENALTGHENEVVSAQTDTVTDTAALVTVVALVTLHTDAL"
BASE COUNT           72 a           71 c           93 g           53 t
ORIGIN      
        1 agtccatgac gaaagatgaa ctgattgccc gtctccgctc gctgggtgaa caactgaacc
       61 gtgatgtcag cctgacgggg acgaaagaag aactggcgct ccgtgtggca gagctggaag
      121 aggagcttga tgacacggat gacgcagccg gtcaggacac gtctgtcagc ccggaaaatg
      181 cgctgaccgg acatgaaaat gaggtggtat cagcacagac ggataccgtg actgatacgg
      241 ctgctctggt cacggttgtg gcactggtga cgctgcatac cgatgcact
//
LOCUS       DAZKHF020000873          289 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2201S31241
            SAMN43267800-rid25106413.denovo.880, whole genome shotgun sequence.
ACCESSION   DAZKHF020000873 DAZKHF020000000
VERSION     DAZKHF020000873.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267800
            Sequence Read Archive: SRR30307263
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 289)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 289)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 289)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350581.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350581.2
            Genome Coverage       :: 151x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:21:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,652
            CDSs (total)                      :: 5,557
            Genes (coding)                    :: 5,320
            CDSs (with protein)               :: 5,320
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 237
            CDSs (without protein)            :: 237
            Pseudo Genes (ambiguous residues) :: 0 of 237
            Pseudo Genes (frameshifted)       :: 102 of 237
            Pseudo Genes (incomplete)         :: 130 of 237
            Pseudo Genes (internal stop)      :: 52 of 237
            Pseudo Genes (multiple problems)  :: 41 of 237
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..289
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267800-rid25106413.denovo.880"
                     /serotype="O 26:H11"
                     /isolate="2201S31241"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-01"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            <1..>289
                     /locus_tag="ACHOSC_RS27950"
                     /old_locus_tag="pgaptmp_005590"
     CDS             <1..>289
                     /locus_tag="ACHOSC_RS27950"
                     /old_locus_tag="pgaptmp_005590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416772.1"
                     /note="ElaD; specific cysteine protease which targets
                     ubiquitin and ubiquitin-like proteins covalently bound to
                     target proteins; SseL in Salmonella is required for
                     macrophage killing and virulence, and it is secreted by
                     the salmonella pathogenicity island 2 type III secretion
                     system; Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deubiquitinase"
                     /protein_id="HGU4551277.1"
                     /translation="FWDKNRQLTTDEIACYLQKIAANAKNTQVNYPTGLYVPYSTRTH
                     LEDALNENIKSDPSWPNEVQLFHINTGGHWILVSLQKIVNKKNNKLQIKCVI"
BASE COUNT          110 a           63 c           44 g           72 t
ORIGIN      
        1 ttctgggata aaaatcgtca attaaccaca gatgaaatag cttgctatct tcagaagatc
       61 gccgctaatg caaaaaatac tcaagtcaat tatcctactg gtctctacgt cccctactcc
      121 accagaactc acctggaaga cgctctcaat gaaaatatta agagcgatcc atcatggccg
      181 aatgaagtcc agttattcca cataaatact ggcggacact ggatattagt ttcgctacag
      241 aaaatagtaa ataaaaaaaa taataaacta caaataaaat gcgtcatat
//
LOCUS       DAZKHF020000874          288 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2201S31241
            SAMN43267800-rid25106413.denovo.881, whole genome shotgun sequence.
ACCESSION   DAZKHF020000874 DAZKHF020000000
VERSION     DAZKHF020000874.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267800
            Sequence Read Archive: SRR30307263
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 288)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 288)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 288)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350581.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350581.2
            Genome Coverage       :: 151x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:21:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,652
            CDSs (total)                      :: 5,557
            Genes (coding)                    :: 5,320
            CDSs (with protein)               :: 5,320
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 237
            CDSs (without protein)            :: 237
            Pseudo Genes (ambiguous residues) :: 0 of 237
            Pseudo Genes (frameshifted)       :: 102 of 237
            Pseudo Genes (incomplete)         :: 130 of 237
            Pseudo Genes (internal stop)      :: 52 of 237
            Pseudo Genes (multiple problems)  :: 41 of 237
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..288
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267800-rid25106413.denovo.881"
                     /serotype="O 26:H11"
                     /isolate="2201S31241"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-01"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            complement(<1..286)
                     /gene="ler"
                     /locus_tag="ACHOSC_RS27955"
                     /old_locus_tag="pgaptmp_005591"
     CDS             complement(<1..286)
                     /gene="ler"
                     /locus_tag="ACHOSC_RS27955"
                     /old_locus_tag="pgaptmp_005591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312615.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system LEE master regulator
                     Ler"
                     /protein_id="HGU4551278.1"
                     /translation="MEQIEVLQQEAKRLREQEIQSVIESIQKQITYYNITLQELGYTN
                     VPDDGLARRNSSKGVYYRNEEGQTWSGVGRQPRWLKEALLNGMKKEDFLVK"
BASE COUNT           68 a           76 c           48 g           96 t
ORIGIN      
        1 ccttcacaag aaaatcttct ttcttcattc cattcaacag tgcttcttta agccagcgtg
       61 gctgtcggcc aactcccgac caggtctgcc cttcttcatt gcgataataa actcctttcg
      121 atgagttccg gcgagcaagg ccatcatcag gcacattagt atatcccagc tcttgtaggg
      181 ttatattgta ataagtaatc tgcttttgaa tcgactcaat tacactttga atttcctgct
      241 ctcgcagtcg ctttgcttcc tgttgtaaca cttcaatttg ctcaataa
//
LOCUS       DAZKHF020000875          287 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2201S31241
            SAMN43267800-rid25106413.denovo.882, whole genome shotgun sequence.
ACCESSION   DAZKHF020000875 DAZKHF020000000
VERSION     DAZKHF020000875.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267800
            Sequence Read Archive: SRR30307263
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 287)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 287)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 287)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350581.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350581.2
            Genome Coverage       :: 151x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:21:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,652
            CDSs (total)                      :: 5,557
            Genes (coding)                    :: 5,320
            CDSs (with protein)               :: 5,320
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 237
            CDSs (without protein)            :: 237
            Pseudo Genes (ambiguous residues) :: 0 of 237
            Pseudo Genes (frameshifted)       :: 102 of 237
            Pseudo Genes (incomplete)         :: 130 of 237
            Pseudo Genes (internal stop)      :: 52 of 237
            Pseudo Genes (multiple problems)  :: 41 of 237
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..287
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267800-rid25106413.denovo.882"
                     /serotype="O 26:H11"
                     /isolate="2201S31241"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-01"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            81..>287
                     /gene="dinJ"
                     /locus_tag="ACHOSC_RS27960"
                     /old_locus_tag="pgaptmp_005592"
     CDS             81..>287
                     /gene="dinJ"
                     /locus_tag="ACHOSC_RS27960"
                     /old_locus_tag="pgaptmp_005592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system antitoxin DinJ"
                     /protein_id="HGU4551279.1"
                     /translation="MAANAFVRARIDEDLKNQAADVLAGMGLTISDLVRITLTKVARE
                     KALPFDLREPNQLTIQSIKNSEAGV"
BASE COUNT           82 a           69 c           68 g           68 t
ORIGIN      
        1 aaattactca tggattgtcg ctgttgctca tttgagctac aattcaagct gaataaatat
       61 acagcacagg agatacccca atggctgcta acgcatttgt tcgcgcccga atcgatgaag
      121 atctgaagaa tcaggcggcg gacgtactgg ccgggatggg gctgaccatc tctgacctgg
      181 ttcgcataac cctcacaaag gtcgcgcgtg aaaaggcatt gccgtttgat ttacgcgagc
      241 ctaatcaatt aaccattcaa tcaatcaaaa acagcgaagc tggcgtt
//
LOCUS       DAZKHF020000876          287 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2201S31241
            SAMN43267800-rid25106413.denovo.883, whole genome shotgun sequence.
ACCESSION   DAZKHF020000876 DAZKHF020000000
VERSION     DAZKHF020000876.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267800
            Sequence Read Archive: SRR30307263
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 287)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 287)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 287)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350581.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350581.2
            Genome Coverage       :: 151x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:21:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,652
            CDSs (total)                      :: 5,557
            Genes (coding)                    :: 5,320
            CDSs (with protein)               :: 5,320
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 237
            CDSs (without protein)            :: 237
            Pseudo Genes (ambiguous residues) :: 0 of 237
            Pseudo Genes (frameshifted)       :: 102 of 237
            Pseudo Genes (incomplete)         :: 130 of 237
            Pseudo Genes (internal stop)      :: 52 of 237
            Pseudo Genes (multiple problems)  :: 41 of 237
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..287
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267800-rid25106413.denovo.883"
                     /serotype="O 26:H11"
                     /isolate="2201S31241"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-01"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            <1..>287
                     /locus_tag="ACHOSC_RS27965"
                     /old_locus_tag="pgaptmp_005593"
     rRNA            <1..>287
                     /locus_tag="ACHOSC_RS27965"
                     /old_locus_tag="pgaptmp_005593"
                     /product="23S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF02541"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF02541"
BASE COUNT           87 a           67 c           80 g           53 t
ORIGIN      
        1 cctgcttcca ggaaaagcct ctaagcatca ggtaacatca aatcgtaccc caaaccgaca
       61 caggtggtca ggtagagaat accaaggcgc ttgagagaac tcgggtgaag gaactaggca
      121 aaatggtgcc gtaacttcgg gagaaggcac gctgatatgt aggtgaagtc cctcgcggat
      181 ggagctgaaa tcagtcgaag ataccagctg gctgcaactg tttattaaaa acacagcact
      241 gtgcaaacac gaaagtggac gtatacggtg tgacgcctgc ccggtgc
//
LOCUS       DAZKHF020000877          286 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2201S31241
            SAMN43267800-rid25106413.denovo.884, whole genome shotgun sequence.
ACCESSION   DAZKHF020000877 DAZKHF020000000
VERSION     DAZKHF020000877.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267800
            Sequence Read Archive: SRR30307263
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 286)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 286)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 286)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350581.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350581.2
            Genome Coverage       :: 151x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:21:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,652
            CDSs (total)                      :: 5,557
            Genes (coding)                    :: 5,320
            CDSs (with protein)               :: 5,320
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 237
            CDSs (without protein)            :: 237
            Pseudo Genes (ambiguous residues) :: 0 of 237
            Pseudo Genes (frameshifted)       :: 102 of 237
            Pseudo Genes (incomplete)         :: 130 of 237
            Pseudo Genes (internal stop)      :: 52 of 237
            Pseudo Genes (multiple problems)  :: 41 of 237
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..286
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267800-rid25106413.denovo.884"
                     /serotype="O 26:H11"
                     /isolate="2201S31241"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-01"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            <1..>286
                     /locus_tag="ACHOSC_RS27970"
                     /old_locus_tag="pgaptmp_005594"
     CDS             <1..>286
                     /locus_tag="ACHOSC_RS27970"
                     /old_locus_tag="pgaptmp_005594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4551280.1"
                     /translation="NISKDEWENLHALKEKRLNGMPKTTPKSDQVIMLQNQYVREMRK
                     YGVRGLRYDAAKHSKHEQIERSITPPLKNYNERLHNTNLFNPKYHKKAVMN"
BASE COUNT          121 a           52 c           46 g           67 t
ORIGIN      
        1 taacatctct aaggatgaat gggaaaacct ccatgcatta aaagaaaagc gtttaaatgg
       61 aatgcctaaa acaacaccca aaagtgacca ggttattatg ttgcaaaatc aatacgttcg
      121 tgaaatgcga aaatatggcg tacgaggttt acgttatgat gcggcaaaac actcaaaaca
      181 tgaacaaata gaaagatcaa taaccccacc gcttaaaaat tataatgagc ggttacacaa
      241 tactaactta tttaacccaa aatatcataa aaaagccgtt atgaat
//
LOCUS       DAZKHF020000878          285 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2201S31241
            SAMN43267800-rid25106413.denovo.885, whole genome shotgun sequence.
ACCESSION   DAZKHF020000878 DAZKHF020000000
VERSION     DAZKHF020000878.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267800
            Sequence Read Archive: SRR30307263
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 285)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 285)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 285)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350581.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350581.2
            Genome Coverage       :: 151x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:21:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,652
            CDSs (total)                      :: 5,557
            Genes (coding)                    :: 5,320
            CDSs (with protein)               :: 5,320
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 237
            CDSs (without protein)            :: 237
            Pseudo Genes (ambiguous residues) :: 0 of 237
            Pseudo Genes (frameshifted)       :: 102 of 237
            Pseudo Genes (incomplete)         :: 130 of 237
            Pseudo Genes (internal stop)      :: 52 of 237
            Pseudo Genes (multiple problems)  :: 41 of 237
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..285
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267800-rid25106413.denovo.885"
                     /serotype="O 26:H11"
                     /isolate="2201S31241"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-01"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
BASE COUNT           90 a           35 c           66 g           94 t
ORIGIN      
        1 atattatctc cttctacaca acaattcgtt ccgttaagga aaaagattgg tgtcacaagt
       61 atttatttat gattaatcag agggtttatg acagagaaaa ggatgtatat ttctgagtaa
      121 acatgtacag gcctatgggg tgactattta atgagtaaat gccgaatgat tttcggggtg
      181 atgattataa cttgaggaaa aatcccagcg tggtttgagt agtgtacctg agtgatattt
      241 gtgttatgta tgcattgatt gcagcagaat aaatatagtt caggc
//
LOCUS       DAZKHF020000879          285 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2201S31241
            SAMN43267800-rid25106413.denovo.886, whole genome shotgun sequence.
ACCESSION   DAZKHF020000879 DAZKHF020000000
VERSION     DAZKHF020000879.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267800
            Sequence Read Archive: SRR30307263
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 285)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 285)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 285)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350581.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350581.2
            Genome Coverage       :: 151x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:21:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,652
            CDSs (total)                      :: 5,557
            Genes (coding)                    :: 5,320
            CDSs (with protein)               :: 5,320
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 237
            CDSs (without protein)            :: 237
            Pseudo Genes (ambiguous residues) :: 0 of 237
            Pseudo Genes (frameshifted)       :: 102 of 237
            Pseudo Genes (incomplete)         :: 130 of 237
            Pseudo Genes (internal stop)      :: 52 of 237
            Pseudo Genes (multiple problems)  :: 41 of 237
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..285
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267800-rid25106413.denovo.886"
                     /serotype="O 26:H11"
                     /isolate="2201S31241"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-01"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            64..>285
                     /locus_tag="ACHOSC_RS27975"
                     /old_locus_tag="pgaptmp_005595"
     CDS             64..>285
                     /locus_tag="ACHOSC_RS27975"
                     /old_locus_tag="pgaptmp_005595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707761.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NapC/NirT family cytochrome c"
                     /protein_id="HGU4551281.1"
                     /translation="MRGKKRIGLLFLLIAVVVGGGGLLLAQKALHKTSDTAFCLSCHS
                     MSKPFEEYQGTVHFSNQKGIRAECADCHIP"
BASE COUNT           74 a           47 c           73 g           91 t
ORIGIN      
        1 taattcgttg tgtatttaat tatacaatga tttcggtgtt ctggaattta tttagaggaa
       61 tctatgcgag ggaaaaaacg cattgggtta ttgtttttgc tgatagcggt tgtggttggt
      121 ggcggcgggt tattgctggc gcaaaaagcc ttacataaaa cgtcggatac agcattttgc
      181 ctttcctgcc actcgatgag taaacctttt gaggaatatc agggaactgt ccacttttcg
      241 aaccagaaag ggatacgtgc ggaatgtgca gattgccata ttcca
//
LOCUS       DAZKHF020000880          284 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2201S31241
            SAMN43267800-rid25106413.denovo.887, whole genome shotgun sequence.
ACCESSION   DAZKHF020000880 DAZKHF020000000
VERSION     DAZKHF020000880.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267800
            Sequence Read Archive: SRR30307263
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 284)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 284)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 284)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350581.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350581.2
            Genome Coverage       :: 151x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:21:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,652
            CDSs (total)                      :: 5,557
            Genes (coding)                    :: 5,320
            CDSs (with protein)               :: 5,320
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 237
            CDSs (without protein)            :: 237
            Pseudo Genes (ambiguous residues) :: 0 of 237
            Pseudo Genes (frameshifted)       :: 102 of 237
            Pseudo Genes (incomplete)         :: 130 of 237
            Pseudo Genes (internal stop)      :: 52 of 237
            Pseudo Genes (multiple problems)  :: 41 of 237
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..284
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267800-rid25106413.denovo.887"
                     /serotype="O 26:H11"
                     /isolate="2201S31241"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-01"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            <1..>284
                     /locus_tag="ACHOSC_RS27980"
                     /old_locus_tag="pgaptmp_005596"
     CDS             <1..>284
                     /locus_tag="ACHOSC_RS27980"
                     /old_locus_tag="pgaptmp_005596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="phage tail tape measure C-terminal
                     domain-containing protein"
                     /protein_id="HGU4551282.1"
                     /translation="ADKFAQQQRAKRAAIDAKSRGLTDRQAEREATEQRLKEQYGDNP
                     LALNNVMSEQKKTWAAEDQLRGSWMAGLKSGWSEWEESATDSMSQVKSAA"
BASE COUNT           81 a           67 c          101 g           35 t
ORIGIN      
        1 aggcggataa attcgcacag cagcaacggg caaaacgggc cgccattgat gcgaaaagcc
       61 gggggctgac tgaccggcag gcagaacggg aagccacgga acagcgcctg aaggaacagt
      121 atggcgataa tccgctggcg ctgaataacg tcatgtcaga gcagaaaaag acctgggcgg
      181 ctgaagacca gcttcgcggg agctggatgg caggcctgaa gtccggctgg agtgagtggg
      241 aagagagcgc cacggacagt atgtcgcagg taaaaagtgc agcc
//
LOCUS       DAZKHF020000881          284 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2201S31241
            SAMN43267800-rid25106413.denovo.888, whole genome shotgun sequence.
ACCESSION   DAZKHF020000881 DAZKHF020000000
VERSION     DAZKHF020000881.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267800
            Sequence Read Archive: SRR30307263
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 284)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 284)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 284)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350581.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350581.2
            Genome Coverage       :: 151x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:21:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,652
            CDSs (total)                      :: 5,557
            Genes (coding)                    :: 5,320
            CDSs (with protein)               :: 5,320
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 237
            CDSs (without protein)            :: 237
            Pseudo Genes (ambiguous residues) :: 0 of 237
            Pseudo Genes (frameshifted)       :: 102 of 237
            Pseudo Genes (incomplete)         :: 130 of 237
            Pseudo Genes (internal stop)      :: 52 of 237
            Pseudo Genes (multiple problems)  :: 41 of 237
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..284
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267800-rid25106413.denovo.888"
                     /serotype="O 26:H11"
                     /isolate="2201S31241"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-01"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            <1..>284
                     /locus_tag="ACHOSC_RS27985"
                     /old_locus_tag="pgaptmp_005597"
     CDS             <1..>284
                     /locus_tag="ACHOSC_RS27985"
                     /old_locus_tag="pgaptmp_005597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708766.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4551283.1"
                     /translation="HLTYTQARNITRFGTIESVTYDIAEGSVVSLAAGGISFALTASV
                     FWLSTGDRDAALQTAAVQAGKTFTRTLAVYVTTQQLHRLSVVQGMLKHID"
BASE COUNT           57 a           84 c           75 g           68 t
ORIGIN      
        1 catctgactt atactcaggc ccgtaatatc acccggttcg ggaccatcga atcggtcact
       61 tatgatattg ccgagggttc ggttgtcagt ctggcggccg gagggatcag ttttgccctg
      121 acggcatcgg tcttctggct cagcaccggc gatcgcgatg ctgccctgca gacagctgct
      181 gtccaggcag gaaaaacctt cacccgcaca ctggctgtct acgtcacaac ccagcaactc
      241 caccggctca gtgttgttca gggtatgctg aagcacattg attt
//
LOCUS       DAZKHF020000882          282 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2201S31241
            SAMN43267800-rid25106413.denovo.889, whole genome shotgun sequence.
ACCESSION   DAZKHF020000882 DAZKHF020000000
VERSION     DAZKHF020000882.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267800
            Sequence Read Archive: SRR30307263
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 282)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 282)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 282)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350581.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350581.2
            Genome Coverage       :: 151x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:21:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,652
            CDSs (total)                      :: 5,557
            Genes (coding)                    :: 5,320
            CDSs (with protein)               :: 5,320
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 237
            CDSs (without protein)            :: 237
            Pseudo Genes (ambiguous residues) :: 0 of 237
            Pseudo Genes (frameshifted)       :: 102 of 237
            Pseudo Genes (incomplete)         :: 130 of 237
            Pseudo Genes (internal stop)      :: 52 of 237
            Pseudo Genes (multiple problems)  :: 41 of 237
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..282
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267800-rid25106413.denovo.889"
                     /serotype="O 26:H11"
                     /isolate="2201S31241"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-01"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            <1..>282
                     /locus_tag="ACHOSC_RS27990"
                     /old_locus_tag="pgaptmp_005598"
     CDS             <1..>282
                     /locus_tag="ACHOSC_RS27990"
                     /old_locus_tag="pgaptmp_005598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001239084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4551284.1"
                     /translation="SSLINILKEHYNEDEISAKLPLAYDYILNKKESSSIPVEILNKL
                     SELPPHELLTPVLGQSVNPLGMGYSSDNGKITEQVIVSGADGFDNPISGL"
BASE COUNT          100 a           51 c           48 g           83 t
ORIGIN      
        1 atcgtcctta attaatatat tgaaagaaca ttataatgaa gatgaaatta gtgcgaaatt
       61 accactagca tatgattaca ttttaaataa aaaagaatca agctctattc ctgttgaaat
      121 tttgaacaaa ctttcagagt taccaccaca tgaactactc acacctgttc ttggccagag
      181 tgttaatcct ctgggcatgg gctactcatc cgataatgga aaaatcacag agcaagtaat
      241 agtcagtgga gctgatggat ttgataatcc catatctgga ct
//
LOCUS       DAZKHF020000883          281 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2201S31241
            SAMN43267800-rid25106413.denovo.890, whole genome shotgun sequence.
ACCESSION   DAZKHF020000883 DAZKHF020000000
VERSION     DAZKHF020000883.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267800
            Sequence Read Archive: SRR30307263
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 281)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 281)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 281)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350581.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350581.2
            Genome Coverage       :: 151x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:21:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,652
            CDSs (total)                      :: 5,557
            Genes (coding)                    :: 5,320
            CDSs (with protein)               :: 5,320
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 237
            CDSs (without protein)            :: 237
            Pseudo Genes (ambiguous residues) :: 0 of 237
            Pseudo Genes (frameshifted)       :: 102 of 237
            Pseudo Genes (incomplete)         :: 130 of 237
            Pseudo Genes (internal stop)      :: 52 of 237
            Pseudo Genes (multiple problems)  :: 41 of 237
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..281
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267800-rid25106413.denovo.890"
                     /serotype="O 26:H11"
                     /isolate="2201S31241"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-01"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            11..>281
                     /locus_tag="ACHOSC_RS27995"
                     /old_locus_tag="pgaptmp_005599"
     CDS             11..>281
                     /locus_tag="ACHOSC_RS27995"
                     /old_locus_tag="pgaptmp_005599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LuxR family transcriptional regulator"
                     /protein_id="HGU4551285.1"
                     /translation="MQVFSSDVYFTVGTNALLASQKEYYSDLVALVDLGHSFVVIDEH
                     QHRNLKPNTEPVNILLSNNFIRINKNITLSDLTHFLVSNLHTQNVY"
BASE COUNT           89 a           53 c           46 g           93 t
ORIGIN      
        1 aggtgggcat atgcaagtct tttccagtga tgtctacttc actgtaggca ctaatgcatt
       61 attagcatcc caaaaagaat attacagcga tttagttgcg ttggttgatc ttggacattc
      121 gttcgttgtt attgatgagc atcaacatcg aaatctcaaa ccaaatacag aacctgtaaa
      181 catccttttg agcaacaatt tcatcaggat caataagaat attacattat ctgatttaac
      241 gcatttcctg gtttcaaatc ttcatacaca aaatgtgtat t
//
LOCUS       DAZKHF020000884          279 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2201S31241
            SAMN43267800-rid25106413.denovo.891, whole genome shotgun sequence.
ACCESSION   DAZKHF020000884 DAZKHF020000000
VERSION     DAZKHF020000884.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267800
            Sequence Read Archive: SRR30307263
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 279)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 279)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 279)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350581.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350581.2
            Genome Coverage       :: 151x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:21:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,652
            CDSs (total)                      :: 5,557
            Genes (coding)                    :: 5,320
            CDSs (with protein)               :: 5,320
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 237
            CDSs (without protein)            :: 237
            Pseudo Genes (ambiguous residues) :: 0 of 237
            Pseudo Genes (frameshifted)       :: 102 of 237
            Pseudo Genes (incomplete)         :: 130 of 237
            Pseudo Genes (internal stop)      :: 52 of 237
            Pseudo Genes (multiple problems)  :: 41 of 237
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..279
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267800-rid25106413.denovo.891"
                     /serotype="O 26:H11"
                     /isolate="2201S31241"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-01"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            156..>279
                     /locus_tag="ACHOSC_RS28000"
                     /old_locus_tag="pgaptmp_005600"
     CDS             156..>279
                     /locus_tag="ACHOSC_RS28000"
                     /old_locus_tag="pgaptmp_005600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_490587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="complement resistance protein TraT"
                     /protein_id="HGU4551286.1"
                     /translation="MKTKKVMMIALVSSALALSGCGAMGTAIKKRNLEVKTQMSE"
BASE COUNT           92 a           59 c           57 g           71 t
ORIGIN      
        1 cctaaaaact tcttatgaaa taaacgttta cccgacgtat ctgatcccgc tgtttttacc
       61 tgtatccttt aacatacttg agaagcccgc tgaaaaatat accgtaaagg cggttctctg
      121 tcttccataa acctgtcgga taagaagggc attaaatgaa aaccaaaaaa gttatgatga
      181 ttgcactggt cagctctgcg ctggctcttt caggatgtgg agccatgggc acagcgatca
      241 aaaaacgaaa tctggaagta aaaacccaga tgagtgaaa
//
LOCUS       DAZKHF020000885          279 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2201S31241
            SAMN43267800-rid25106413.denovo.892, whole genome shotgun sequence.
ACCESSION   DAZKHF020000885 DAZKHF020000000
VERSION     DAZKHF020000885.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267800
            Sequence Read Archive: SRR30307263
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 279)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 279)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 279)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350581.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350581.2
            Genome Coverage       :: 151x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:21:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,652
            CDSs (total)                      :: 5,557
            Genes (coding)                    :: 5,320
            CDSs (with protein)               :: 5,320
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 237
            CDSs (without protein)            :: 237
            Pseudo Genes (ambiguous residues) :: 0 of 237
            Pseudo Genes (frameshifted)       :: 102 of 237
            Pseudo Genes (incomplete)         :: 130 of 237
            Pseudo Genes (internal stop)      :: 52 of 237
            Pseudo Genes (multiple problems)  :: 41 of 237
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..279
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267800-rid25106413.denovo.892"
                     /serotype="O 26:H11"
                     /isolate="2201S31241"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-01"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            <1..>279
                     /locus_tag="ACHOSC_RS28005"
                     /old_locus_tag="pgaptmp_005601"
     CDS             <1..>279
                     /locus_tag="ACHOSC_RS28005"
                     /old_locus_tag="pgaptmp_005601"
                     /EC_number="2.7.1.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004143 - ATP-dependent diacylglycerol
                     kinase activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006654 - phosphatidic acid biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="diacylglycerol kinase"
                     /protein_id="HGU4551287.1"
                     /translation="TRIIKAAGYSWKGLCAAWRYEAAFRQECILVLSALIISFWLDVD
                     SVTRVLLIGSAMMVIIVEIINSAIEAVVDRIGSEHHELSGRAKDMGSAA"
BASE COUNT           77 a           47 c           70 g           85 t
ORIGIN      
        1 taccagaatt attaaagcag cgggttattc atggaaaggc ttatgtgcgg catggcgcta
       61 tgaagcagcc tttcgtcagg agtgcattct ggttctgtca gcactgataa tatccttttg
      121 gctggatgtt gactctgtta ctcgagtatt actcattggt tcggcaatga tggtaattat
      181 tgttgaaata attaacagtg caattgaagc agttgttgac agaattggtt cagaacatca
      241 cgaattatca ggcagagcaa aagatatggg ttcagccgc
//
LOCUS       DAZKHF020000886          278 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2201S31241
            SAMN43267800-rid25106413.denovo.893, whole genome shotgun sequence.
ACCESSION   DAZKHF020000886 DAZKHF020000000
VERSION     DAZKHF020000886.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267800
            Sequence Read Archive: SRR30307263
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 278)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 278)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 278)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350581.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350581.2
            Genome Coverage       :: 151x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:21:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,652
            CDSs (total)                      :: 5,557
            Genes (coding)                    :: 5,320
            CDSs (with protein)               :: 5,320
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 237
            CDSs (without protein)            :: 237
            Pseudo Genes (ambiguous residues) :: 0 of 237
            Pseudo Genes (frameshifted)       :: 102 of 237
            Pseudo Genes (incomplete)         :: 130 of 237
            Pseudo Genes (internal stop)      :: 52 of 237
            Pseudo Genes (multiple problems)  :: 41 of 237
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..278
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267800-rid25106413.denovo.893"
                     /serotype="O 26:H11"
                     /isolate="2201S31241"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-01"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            complement(<1..>278)
                     /gene="escQ"
                     /locus_tag="ACHOSC_RS28010"
                     /old_locus_tag="pgaptmp_005602"
     CDS             complement(<1..>278)
                     /gene="escQ"
                     /locus_tag="ACHOSC_RS28010"
                     /old_locus_tag="pgaptmp_005602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="type III secretion system LEE ring protein EscQ"
                     /protein_id="HGU4551288.1"
                     /translation="RLYITPEGHACHAYFREVSGNGIRFTLTCSGYEGRFWISEEQFI
                     QWCQELFPYSESRLIPEDMIKLMILWVMQTALPEGDVSVDDVQFTMLN"
BASE COUNT           82 a           70 c           53 g           73 t
ORIGIN      
        1 tatttagcat cgtaaattga acatcatcaa cagacacatc accttctggt aaagcagtct
       61 gcattaccca taagatcatg agttttatca tatcttccgg tatcagccgg ctttcgctat
      121 acgggaataa ctcctggcac cactgaatga actgttcttc tgatatccaa aaacgcccct
      181 catagcctga gcatgttaac gtgaagcgga taccattacc gctgacttcg cgaaaatatg
      241 catggcaagc atgcccttca ggcgtaatat agaggcga
//
LOCUS       DAZKHF020000887          276 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2201S31241
            SAMN43267800-rid25106413.denovo.894, whole genome shotgun sequence.
ACCESSION   DAZKHF020000887 DAZKHF020000000
VERSION     DAZKHF020000887.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267800
            Sequence Read Archive: SRR30307263
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 276)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 276)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 276)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350581.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350581.2
            Genome Coverage       :: 151x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:21:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,652
            CDSs (total)                      :: 5,557
            Genes (coding)                    :: 5,320
            CDSs (with protein)               :: 5,320
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 237
            CDSs (without protein)            :: 237
            Pseudo Genes (ambiguous residues) :: 0 of 237
            Pseudo Genes (frameshifted)       :: 102 of 237
            Pseudo Genes (incomplete)         :: 130 of 237
            Pseudo Genes (internal stop)      :: 52 of 237
            Pseudo Genes (multiple problems)  :: 41 of 237
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..276
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267800-rid25106413.denovo.894"
                     /serotype="O 26:H11"
                     /isolate="2201S31241"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-01"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            complement(<1..>276)
                     /locus_tag="ACHOSC_RS28015"
                     /old_locus_tag="pgaptmp_005603"
     CDS             complement(<1..>276)
                     /locus_tag="ACHOSC_RS28015"
                     /old_locus_tag="pgaptmp_005603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF3168 domain-containing protein"
                     /protein_id="HGU4551289.1"
                     /translation="QVYPYVVPLLDGRPSVALPWVVFSLISSVSADVMGGQAESSVSV
                     QIDVYAGTVTQARQIRQDAREAIMLLAPGSVSEMQDYIPENRCYRAT"
BASE COUNT           60 a           96 c           73 g           47 t
ORIGIN      
        1 gggttgcacg gtaacagcgg ttttccggaa tatagtcctg catttcactg acggatcccg
       61 gggccagcag cattatggct tcacgggcgt cctgacgtat ctgacgcgcc tgcgtcacag
      121 tcccggcata aacgtctatc tgcaccgaca ctgaggactc cgcctgcccg cccatcacgt
      181 ccgccgacac cgatgaaatc aggctgaaaa ccacccacgg aagcgccacc gacggcctgc
      241 catccagcag ggggaccaca tacgggtaca cctgcc
//
LOCUS       DAZKHF020000888          271 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2201S31241
            SAMN43267800-rid25106413.denovo.895, whole genome shotgun sequence.
ACCESSION   DAZKHF020000888 DAZKHF020000000
VERSION     DAZKHF020000888.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267800
            Sequence Read Archive: SRR30307263
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 271)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 271)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 271)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350581.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350581.2
            Genome Coverage       :: 151x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:21:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,652
            CDSs (total)                      :: 5,557
            Genes (coding)                    :: 5,320
            CDSs (with protein)               :: 5,320
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 237
            CDSs (without protein)            :: 237
            Pseudo Genes (ambiguous residues) :: 0 of 237
            Pseudo Genes (frameshifted)       :: 102 of 237
            Pseudo Genes (incomplete)         :: 130 of 237
            Pseudo Genes (internal stop)      :: 52 of 237
            Pseudo Genes (multiple problems)  :: 41 of 237
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..271
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267800-rid25106413.denovo.895"
                     /serotype="O 26:H11"
                     /isolate="2201S31241"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-01"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            <1..>271
                     /gene="sepL"
                     /locus_tag="ACHOSC_RS28020"
                     /old_locus_tag="pgaptmp_005604"
     CDS             <1..>271
                     /gene="sepL"
                     /locus_tag="ACHOSC_RS28020"
                     /old_locus_tag="pgaptmp_005604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system LEE gatekeeper SepL"
                     /protein_id="HGU4551290.1"
                     /translation="WELALLSWLGVGELNQEGIQKIKKLYEKAKDEDSEKGASLLDWF
                     MEIKDLPEREKHLKVIIRALSFDLSYMSSFEDKVKTSSIISDLCRV"
BASE COUNT           76 a           38 c           73 g           84 t
ORIGIN      
        1 tgggagttgg ctctgctttc ctggttaggg gtgggtgagt taaatcagga aggtatccag
       61 aagatcaaaa agctttatga aaaggccaag gatgaggatt ctgaaaaggg agcctcttta
      121 cttgactggt ttatggagat taaggatctt ccagagcgtg agaagcactt aaaggttatt
      181 attagggcgc tatcgttcga tctgtcttat atgtcttctt ttgaagataa ggtgaaaaca
      241 tcttcaatta ttagcgattt atgccgggta a
//
LOCUS       DAZKHF020000889          268 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2201S31241
            SAMN43267800-rid25106413.denovo.896, whole genome shotgun sequence.
ACCESSION   DAZKHF020000889 DAZKHF020000000
VERSION     DAZKHF020000889.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267800
            Sequence Read Archive: SRR30307263
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 268)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 268)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 268)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350581.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350581.2
            Genome Coverage       :: 151x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:21:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,652
            CDSs (total)                      :: 5,557
            Genes (coding)                    :: 5,320
            CDSs (with protein)               :: 5,320
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 237
            CDSs (without protein)            :: 237
            Pseudo Genes (ambiguous residues) :: 0 of 237
            Pseudo Genes (frameshifted)       :: 102 of 237
            Pseudo Genes (incomplete)         :: 130 of 237
            Pseudo Genes (internal stop)      :: 52 of 237
            Pseudo Genes (multiple problems)  :: 41 of 237
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..268
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267800-rid25106413.denovo.896"
                     /serotype="O 26:H11"
                     /isolate="2201S31241"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-01"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            14..226
                     /locus_tag="ACHOSC_RS28025"
                     /old_locus_tag="pgaptmp_005605"
     CDS             14..226
                     /locus_tag="ACHOSC_RS28025"
                     /old_locus_tag="pgaptmp_005605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4551291.1"
                     /translation="MLNTQKAINAEKYNEWARKFSEQIFKITGDENAAKNELEPWTPE
                     GADPNYCWREVDPVDAANEAMSYHND"
BASE COUNT           93 a           42 c           71 g           62 t
ORIGIN      
        1 tggaggtata tttatgttaa atacacagaa agccattaat gcggaaaaat ataacgagtg
       61 ggcaagaaaa ttctctgagc agatttttaa aattactggc gatgagaatg cggcaaaaaa
      121 tgaattagaa ccgtggacgc ctgaaggagc cgacccaaat tattgctgga gggaggttga
      181 tccagttgat gctgcaaatg aagctatgag ttatcacaac gattaatgtc aggaggccgc
      241 ccgaaagggc ggtaatgaat ggtcacat
//
LOCUS       DAZKHF020000890          262 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2201S31241
            SAMN43267800-rid25106413.denovo.898, whole genome shotgun sequence.
ACCESSION   DAZKHF020000890 DAZKHF020000000
VERSION     DAZKHF020000890.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267800
            Sequence Read Archive: SRR30307263
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 262)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 262)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 262)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350581.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350581.2
            Genome Coverage       :: 151x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:21:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,652
            CDSs (total)                      :: 5,557
            Genes (coding)                    :: 5,320
            CDSs (with protein)               :: 5,320
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 237
            CDSs (without protein)            :: 237
            Pseudo Genes (ambiguous residues) :: 0 of 237
            Pseudo Genes (frameshifted)       :: 102 of 237
            Pseudo Genes (incomplete)         :: 130 of 237
            Pseudo Genes (internal stop)      :: 52 of 237
            Pseudo Genes (multiple problems)  :: 41 of 237
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..262
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267800-rid25106413.denovo.898"
                     /serotype="O 26:H11"
                     /isolate="2201S31241"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-01"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            172..>262
                     /locus_tag="ACHOSC_RS28030"
                     /old_locus_tag="pgaptmp_005606"
     CDS             172..>262
                     /locus_tag="ACHOSC_RS28030"
                     /old_locus_tag="pgaptmp_005606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4551292.1"
                     /translation="MSSKNRTRRTTTRNIRFPNQIIEQINIALD"
BASE COUNT          103 a           59 c           38 g           62 t
ORIGIN      
        1 atgataataa ttatcatcta catgaggttt atcacgacat gtgtgtacgc catcaaacca
       61 cgagaaataa tcaattatta cgcaggtatc gtattaattg atctgcatca aattagcgta
      121 aaagcaactt cagataatac aaatcagcaa cactgaatac ggggcaacat tatgtcatca
      181 aagaacagaa cccgcagaac aacaacccgc aacatccgat ttccaaacca gataattgaa
      241 caaattaaca tcgctcttga cc
//
LOCUS       DAZKHF020000891          261 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2201S31241
            SAMN43267800-rid25106413.denovo.899, whole genome shotgun sequence.
ACCESSION   DAZKHF020000891 DAZKHF020000000
VERSION     DAZKHF020000891.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267800
            Sequence Read Archive: SRR30307263
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 261)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 261)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 261)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350581.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350581.2
            Genome Coverage       :: 151x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:21:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,652
            CDSs (total)                      :: 5,557
            Genes (coding)                    :: 5,320
            CDSs (with protein)               :: 5,320
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 237
            CDSs (without protein)            :: 237
            Pseudo Genes (ambiguous residues) :: 0 of 237
            Pseudo Genes (frameshifted)       :: 102 of 237
            Pseudo Genes (incomplete)         :: 130 of 237
            Pseudo Genes (internal stop)      :: 52 of 237
            Pseudo Genes (multiple problems)  :: 41 of 237
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..261
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267800-rid25106413.denovo.899"
                     /serotype="O 26:H11"
                     /isolate="2201S31241"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-01"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            <1..>261
                     /locus_tag="ACHOSC_RS28035"
                     /old_locus_tag="pgaptmp_005607"
     CDS             <1..>261
                     /locus_tag="ACHOSC_RS28035"
                     /old_locus_tag="pgaptmp_005607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tape measure protein"
                     /protein_id="HGU4551293.1"
                     /translation="VLDIGRPDTAQEMLIKAEAAFKKADDIWNLRKDDYFVNDEARAR
                     YWDDREKARLALEAARKKAEQQTQQDKNAQQQSDTEASRLKYT"
BASE COUNT           74 a           57 c           80 g           50 t
ORIGIN      
        1 gtgctggata ttggtcgtcc tgataccgcg caggagatgc tgattaaggc agaggctgcg
       61 tttaagaaag cagacgacat ctggaatctg cgcaaggatg attattttgt taacgatgaa
      121 gcgcgggcgc gttactggga tgatcgtgaa aaggcccgtc ttgcgcttga agccgcccga
      181 aagaaggctg agcagcagac tcaacaggac aaaaatgcgc agcagcagag cgataccgaa
      241 gcgtcacggc tgaaatatac c
//
LOCUS       DAZKHF020000892          260 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2201S31241
            SAMN43267800-rid25106413.denovo.900, whole genome shotgun sequence.
ACCESSION   DAZKHF020000892 DAZKHF020000000
VERSION     DAZKHF020000892.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267800
            Sequence Read Archive: SRR30307263
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 260)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 260)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 260)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350581.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350581.2
            Genome Coverage       :: 151x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:21:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,652
            CDSs (total)                      :: 5,557
            Genes (coding)                    :: 5,320
            CDSs (with protein)               :: 5,320
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 237
            CDSs (without protein)            :: 237
            Pseudo Genes (ambiguous residues) :: 0 of 237
            Pseudo Genes (frameshifted)       :: 102 of 237
            Pseudo Genes (incomplete)         :: 130 of 237
            Pseudo Genes (internal stop)      :: 52 of 237
            Pseudo Genes (multiple problems)  :: 41 of 237
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..260
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267800-rid25106413.denovo.900"
                     /serotype="O 26:H11"
                     /isolate="2201S31241"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-01"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            <1..159
                     /locus_tag="ACHOSC_RS28040"
                     /old_locus_tag="pgaptmp_005608"
     CDS             <1..159
                     /locus_tag="ACHOSC_RS28040"
                     /old_locus_tag="pgaptmp_005608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0020002 - host cell plasma membrane
                     [Evidence IEA];
                     GO_function: GO:0042802 - identical protein binding
                     [Evidence IEA];
                     GO_function: GO:0140911 - pore-forming activity [Evidence
                     IEA];
                     GO_process: GO:0019835 - cytolysis [Evidence IEA];
                     GO_process: GO:0044659 - viral release from host cell by
                     cytolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class II holin family protein"
                     /protein_id="HGU4551294.1"
                     /translation="TGYWFLQLLDRVSPSQWAAIGVLGSLLFGLLTYLTNLYFKIRED
                     RRKAARGE"
     gene            164..>260
                     /locus_tag="ACHOSC_RS28045"
                     /old_locus_tag="pgaptmp_005609"
     CDS             164..>260
                     /locus_tag="ACHOSC_RS28045"
                     /old_locus_tag="pgaptmp_005609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysozyme"
                     /protein_id="HGU4551295.1"
                     /translation="MSRKLRYGLSAAVLALIAAGASAPEILDQFLD"
BASE COUNT           48 a           62 c           86 g           64 t
ORIGIN      
        1 acgggctact ggttcctgca gttgctggac agggtttccc cgtctcagtg ggcggcaata
       61 ggcgtgctgg ggagtctgct gtttggtctg ctgacgtacc tgacgaacct gtatttcaaa
      121 atcagagagg acaggcgtaa ggcggcacgg ggagagtaag ctgatgagca ggaaactccg
      181 ctatggttta tcggctgccg ttctggcgct gattgccgca ggtgcttctg cgcctgaaat
      241 cctcgaccag tttctggatg
//
LOCUS       DAZKHF020000893          259 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2201S31241
            SAMN43267800-rid25106413.denovo.901, whole genome shotgun sequence.
ACCESSION   DAZKHF020000893 DAZKHF020000000
VERSION     DAZKHF020000893.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267800
            Sequence Read Archive: SRR30307263
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 259)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 259)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 259)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350581.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350581.2
            Genome Coverage       :: 151x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:21:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,652
            CDSs (total)                      :: 5,557
            Genes (coding)                    :: 5,320
            CDSs (with protein)               :: 5,320
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 237
            CDSs (without protein)            :: 237
            Pseudo Genes (ambiguous residues) :: 0 of 237
            Pseudo Genes (frameshifted)       :: 102 of 237
            Pseudo Genes (incomplete)         :: 130 of 237
            Pseudo Genes (internal stop)      :: 52 of 237
            Pseudo Genes (multiple problems)  :: 41 of 237
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..259
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267800-rid25106413.denovo.901"
                     /serotype="O 26:H11"
                     /isolate="2201S31241"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-01"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            <1..>259
                     /locus_tag="ACHOSC_RS28050"
                     /old_locus_tag="pgaptmp_005610"
     CDS             <1..>259
                     /locus_tag="ACHOSC_RS28050"
                     /old_locus_tag="pgaptmp_005610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001560258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF2713 family protein"
                     /protein_id="HGU4551296.1"
                     /translation="AQFEKLSQRDMKKYLGEMKRLADGGEMNFDGYLDKMSHLVNEGT
                     LDPDILSKMRDAAPQLISFAKSFDPTSKEEIKILTDTSKLI"
BASE COUNT           89 a           42 c           59 g           69 t
ORIGIN      
        1 aagctcagtt tgaaaaatta tctcaacgtg atatgaagaa gtacctggga gagatgaagc
       61 gtctggctga tgggggagaa atgaattttg atggctatct ggataagatg tcacatctgg
      121 tgaatgaagg aacgctcgat cctgatattt taagcaaaat gcgagatgct gcaccacaat
      181 taattagctt cgcgaagtcg tttgacccaa cctcaaagga agagattaaa atacttacag
      241 acacttctaa attaattta
//
LOCUS       DAZKHF020000894          259 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2201S31241
            SAMN43267800-rid25106413.denovo.902, whole genome shotgun sequence.
ACCESSION   DAZKHF020000894 DAZKHF020000000
VERSION     DAZKHF020000894.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267800
            Sequence Read Archive: SRR30307263
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 259)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 259)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 259)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350581.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350581.2
            Genome Coverage       :: 151x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:21:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,652
            CDSs (total)                      :: 5,557
            Genes (coding)                    :: 5,320
            CDSs (with protein)               :: 5,320
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 237
            CDSs (without protein)            :: 237
            Pseudo Genes (ambiguous residues) :: 0 of 237
            Pseudo Genes (frameshifted)       :: 102 of 237
            Pseudo Genes (incomplete)         :: 130 of 237
            Pseudo Genes (internal stop)      :: 52 of 237
            Pseudo Genes (multiple problems)  :: 41 of 237
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..259
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267800-rid25106413.denovo.902"
                     /serotype="O 26:H11"
                     /isolate="2201S31241"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-01"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            <1..192
                     /locus_tag="ACHOSC_RS28055"
                     /old_locus_tag="pgaptmp_005611"
     CDS             <1..192
                     /locus_tag="ACHOSC_RS28055"
                     /old_locus_tag="pgaptmp_005611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001751687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family insertion sequence element accessory
                     protein TnpB"
                     /protein_id="HGU4551297.1"
                     /translation="PSSESPQLLPVTLDAAAEQPESLAEDPETLSISCEVTFRHGTLR
                     FNGNVSEKLLTLLIQELKR"
BASE COUNT           63 a           76 c           65 g           55 t
ORIGIN      
        1 ccttcttcag agagccccca gctacttcct gtgactctcg atgcagctgc cgaacagcca
       61 gaatcgctcg cagaggaccc ggaaaccctc agtatcagct gtgaggtaac gttccggcac
      121 gggacgctcc gcttcaatgg caatgtcagc gaaaagctcc tgactctgct gatacaggaa
      181 ctgaagcgat gatcccgtta ccttccggga ccaaaatttg gctggttgcc ggtatcaccg
      241 atatgagaaa tggcttcaa
//
LOCUS       DAZKHF020000895          255 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2201S31241
            SAMN43267800-rid25106413.denovo.904, whole genome shotgun sequence.
ACCESSION   DAZKHF020000895 DAZKHF020000000
VERSION     DAZKHF020000895.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267800
            Sequence Read Archive: SRR30307263
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 255)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 255)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 255)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350581.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350581.2
            Genome Coverage       :: 151x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:21:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,652
            CDSs (total)                      :: 5,557
            Genes (coding)                    :: 5,320
            CDSs (with protein)               :: 5,320
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 237
            CDSs (without protein)            :: 237
            Pseudo Genes (ambiguous residues) :: 0 of 237
            Pseudo Genes (frameshifted)       :: 102 of 237
            Pseudo Genes (incomplete)         :: 130 of 237
            Pseudo Genes (internal stop)      :: 52 of 237
            Pseudo Genes (multiple problems)  :: 41 of 237
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..255
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267800-rid25106413.denovo.904"
                     /serotype="O 26:H11"
                     /isolate="2201S31241"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-01"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            13..>255
                     /locus_tag="ACHOSC_RS28060"
                     /old_locus_tag="pgaptmp_005612"
     CDS             13..>255
                     /locus_tag="ACHOSC_RS28060"
                     /old_locus_tag="pgaptmp_005612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000060689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="invasion protein"
                     /protein_id="HGU4551298.1"
                     /translation="MSLVGYNSNIIITIALISLLSACTRENSLKENQNKRSGESQAIV
                     DARQFFSEHPEYISSAELEQKLYREFAQVTTVPAYKE"
BASE COUNT           96 a           46 c           52 g           61 t
ORIGIN      
        1 gagaaagttg atatgtcatt agtaggatat aatagtaaca tcataataac catcgcgctg
       61 atatcgttgc tctctgcatg caccagagaa aattcattaa aggagaatca aaataaaaga
      121 agtggcgaat cccaagcgat agtagatgca agacagttct ttagcgaaca cccggaatat
      181 atatcatcag cagaactgga gcaaaagcta tatcgcgaat ttgcacaggt tactacagtt
      241 ccagcttata aagag
//
LOCUS       DAZKHF020000896          254 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2201S31241
            SAMN43267800-rid25106413.denovo.905, whole genome shotgun sequence.
ACCESSION   DAZKHF020000896 DAZKHF020000000
VERSION     DAZKHF020000896.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267800
            Sequence Read Archive: SRR30307263
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 254)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 254)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 254)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350581.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350581.2
            Genome Coverage       :: 151x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:21:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,652
            CDSs (total)                      :: 5,557
            Genes (coding)                    :: 5,320
            CDSs (with protein)               :: 5,320
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 237
            CDSs (without protein)            :: 237
            Pseudo Genes (ambiguous residues) :: 0 of 237
            Pseudo Genes (frameshifted)       :: 102 of 237
            Pseudo Genes (incomplete)         :: 130 of 237
            Pseudo Genes (internal stop)      :: 52 of 237
            Pseudo Genes (multiple problems)  :: 41 of 237
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..254
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267800-rid25106413.denovo.905"
                     /serotype="O 26:H11"
                     /isolate="2201S31241"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-01"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            <1..>254
                     /locus_tag="ACHOSC_RS28065"
                     /old_locus_tag="pgaptmp_005613"
     CDS             <1..>254
                     /locus_tag="ACHOSC_RS28065"
                     /old_locus_tag="pgaptmp_005613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001626253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="Imm57 family immunity protein"
                     /protein_id="HGU4551299.1"
                     /translation="SISASAVTARQFQRELDLADKAILWALISASTKEGRKACSLSYF
                     ACKAAEAELGLAYMAANDNKEFLTSLSNIMRYKIDAGLSE"
BASE COUNT           82 a           47 c           58 g           67 t
ORIGIN      
        1 tctctatttc tgcgagtgca gttactgcaa gacaatttca gagagagtta gatttggcag
       61 acaaagcaat attatgggca ctaatatcag ccagtactaa agagggacga aaagcgtgtt
      121 cactaagcta ttttgcctgt aaggccgcag aggctgaact cggactggca tatatggctg
      181 cgaatgataa caaggaattt ctcacatcct tatctaatat aatgaggtac aaaatagatg
      241 ctggactttc cgaa
//
LOCUS       DAZKHF020000897          252 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2201S31241
            SAMN43267800-rid25106413.denovo.906, whole genome shotgun sequence.
ACCESSION   DAZKHF020000897 DAZKHF020000000
VERSION     DAZKHF020000897.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267800
            Sequence Read Archive: SRR30307263
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 252)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 252)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 252)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350581.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350581.2
            Genome Coverage       :: 151x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:21:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,652
            CDSs (total)                      :: 5,557
            Genes (coding)                    :: 5,320
            CDSs (with protein)               :: 5,320
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 237
            CDSs (without protein)            :: 237
            Pseudo Genes (ambiguous residues) :: 0 of 237
            Pseudo Genes (frameshifted)       :: 102 of 237
            Pseudo Genes (incomplete)         :: 130 of 237
            Pseudo Genes (internal stop)      :: 52 of 237
            Pseudo Genes (multiple problems)  :: 41 of 237
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..252
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267800-rid25106413.denovo.906"
                     /serotype="O 26:H11"
                     /isolate="2201S31241"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-01"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            <1..>252
                     /locus_tag="ACHOSC_RS28070"
                     /old_locus_tag="pgaptmp_005614"
     CDS             <1..>252
                     /locus_tag="ACHOSC_RS28070"
                     /old_locus_tag="pgaptmp_005614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4551300.1"
                     /translation="SANIDRLRVTFGVQSLVQTTSKGDRNPTSVRLLIQLQRNGNWVT
                     EKDVTINGKTTSQFLASVILDNLPERPFNIRMVRETADST"
BASE COUNT           58 a           72 c           73 g           49 t
ORIGIN      
        1 tccgcgaaca ttgaccgcct gcgggtcacc ttcggggtgc agtcactggt gcagaccacg
       61 tcaaagggtg accgaaaccc gacatccgtc cgactgctga ttcagttaca gcgtaacggt
      121 aactgggtga cggaaaagga tgtcaccatt aacggcaaga ccacctcgca gtttctggcg
      181 tcggtgattc tggataatct gcctgagcgg ccctttaaca tccggatggt ccgggagaca
      241 gcggacagca cc
//
LOCUS       DAZKHF020000898          248 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2201S31241
            SAMN43267800-rid25106413.denovo.907, whole genome shotgun sequence.
ACCESSION   DAZKHF020000898 DAZKHF020000000
VERSION     DAZKHF020000898.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267800
            Sequence Read Archive: SRR30307263
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 248)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 248)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 248)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350581.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350581.2
            Genome Coverage       :: 151x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:21:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,652
            CDSs (total)                      :: 5,557
            Genes (coding)                    :: 5,320
            CDSs (with protein)               :: 5,320
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 237
            CDSs (without protein)            :: 237
            Pseudo Genes (ambiguous residues) :: 0 of 237
            Pseudo Genes (frameshifted)       :: 102 of 237
            Pseudo Genes (incomplete)         :: 130 of 237
            Pseudo Genes (internal stop)      :: 52 of 237
            Pseudo Genes (multiple problems)  :: 41 of 237
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..248
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267800-rid25106413.denovo.907"
                     /serotype="O 26:H11"
                     /isolate="2201S31241"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-01"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            <1..90
                     /locus_tag="ACHOSC_RS28075"
                     /old_locus_tag="pgaptmp_005615"
     CDS             <1..90
                     /locus_tag="ACHOSC_RS28075"
                     /old_locus_tag="pgaptmp_005615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4551301.1"
                     /translation="GHRVSLPLSVFNPQDYHPLLITVSGKNVN"
BASE COUNT           57 a           64 c           58 g           69 t
ORIGIN      
        1 ggccatcgtg tcagtctgcc gttatcggtt ttcaatcccc aggactacca tccactgctg
       61 attaccgttt ccggaaaaaa cgttaactga tccctgaagt cagtttcatg cttcagcctc
      121 tgtcacaacg cgtggcagag gctttttatt tatcaggaga aaacagatat gccgggttac
      181 acagagtatg tcctggctga aggatctttc tcatacgggc aggcggttgc tgtcataacc
      241 gcttaccg
//
LOCUS       DAZKHF020000899          247 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2201S31241
            SAMN43267800-rid25106413.denovo.908, whole genome shotgun sequence.
ACCESSION   DAZKHF020000899 DAZKHF020000000
VERSION     DAZKHF020000899.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267800
            Sequence Read Archive: SRR30307263
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 247)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 247)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 247)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350581.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350581.2
            Genome Coverage       :: 151x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:21:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,652
            CDSs (total)                      :: 5,557
            Genes (coding)                    :: 5,320
            CDSs (with protein)               :: 5,320
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 237
            CDSs (without protein)            :: 237
            Pseudo Genes (ambiguous residues) :: 0 of 237
            Pseudo Genes (frameshifted)       :: 102 of 237
            Pseudo Genes (incomplete)         :: 130 of 237
            Pseudo Genes (internal stop)      :: 52 of 237
            Pseudo Genes (multiple problems)  :: 41 of 237
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..247
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267800-rid25106413.denovo.908"
                     /serotype="O 26:H11"
                     /isolate="2201S31241"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-01"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            complement(<1..>247)
                     /locus_tag="ACHOSC_RS28080"
                     /old_locus_tag="pgaptmp_005616"
     CDS             complement(<1..>247)
                     /locus_tag="ACHOSC_RS28080"
                     /old_locus_tag="pgaptmp_005616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000612800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4551302.1"
                     /translation="SSTSEPTASTSSNRSIKLAASTNVYPITRNDSELTLNDFLDNSS
                     STSSLDYINELGSQLTLNDFLDNIKTNEVDRTCTDVVI"
BASE COUNT           71 a           43 c           54 g           79 t
ORIGIN      
        1 aattaccaca tccgtacatg ttctatccac ctcatttgtc tttatgttgt caagaaaatc
       61 atttaacgtc agttgagaac ccaattcatt aatgtagtcc aatgatgacg tagaagagct
      121 attatcaaga aaatcgttca gagtgagctc ggagtcgttt cttgttatcg gatatacatt
      181 tgtggaagcg gctaatttaa tactccggtt gctggaggta gaagctgttg gttcagaggt
      241 tgacgaa
//
LOCUS       DAZKHF020000900          244 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2201S31241
            SAMN43267800-rid25106413.denovo.909, whole genome shotgun sequence.
ACCESSION   DAZKHF020000900 DAZKHF020000000
VERSION     DAZKHF020000900.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267800
            Sequence Read Archive: SRR30307263
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 244)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 244)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 244)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350581.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350581.2
            Genome Coverage       :: 151x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:21:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,652
            CDSs (total)                      :: 5,557
            Genes (coding)                    :: 5,320
            CDSs (with protein)               :: 5,320
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 237
            CDSs (without protein)            :: 237
            Pseudo Genes (ambiguous residues) :: 0 of 237
            Pseudo Genes (frameshifted)       :: 102 of 237
            Pseudo Genes (incomplete)         :: 130 of 237
            Pseudo Genes (internal stop)      :: 52 of 237
            Pseudo Genes (multiple problems)  :: 41 of 237
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..244
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267800-rid25106413.denovo.909"
                     /serotype="O 26:H11"
                     /isolate="2201S31241"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-01"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            <1..>244
                     /locus_tag="ACHOSC_RS28085"
                     /old_locus_tag="pgaptmp_005617"
     CDS             <1..>244
                     /locus_tag="ACHOSC_RS28085"
                     /old_locus_tag="pgaptmp_005617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RHS repeat protein"
                     /protein_id="HGU4551303.1"
                     /translation="ALDRLVQQGGFDGRTQRYHYDLTGKLTQSEDEGLVTLWYYDESD
                     RITHRTVNGEPAEQWQYDDHGWLTDISHLSEGHRVAV"
BASE COUNT           57 a           68 c           77 g           42 t
ORIGIN      
        1 gcgctggacc ggttggtaca gcagggcggc tttgacgggc ggacgcaacg ttatcattat
       61 gacctgaccg gaaaactcac gcagagtgaa gatgaggggc ttgtcaccct ctggtactac
      121 gatgaatcgg accgtatcac tcaccgcacg gtgaacggcg aaccggcaga gcagtggcag
      181 tatgatgacc acggctggct gacagacatc agccacctga gcgaaggcca ccgtgtcgct
      241 gtcc
//
LOCUS       DAZKHF020000901          244 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2201S31241
            SAMN43267800-rid25106413.denovo.910, whole genome shotgun sequence.
ACCESSION   DAZKHF020000901 DAZKHF020000000
VERSION     DAZKHF020000901.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267800
            Sequence Read Archive: SRR30307263
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 244)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 244)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 244)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350581.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350581.2
            Genome Coverage       :: 151x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:21:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,652
            CDSs (total)                      :: 5,557
            Genes (coding)                    :: 5,320
            CDSs (with protein)               :: 5,320
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 237
            CDSs (without protein)            :: 237
            Pseudo Genes (ambiguous residues) :: 0 of 237
            Pseudo Genes (frameshifted)       :: 102 of 237
            Pseudo Genes (incomplete)         :: 130 of 237
            Pseudo Genes (internal stop)      :: 52 of 237
            Pseudo Genes (multiple problems)  :: 41 of 237
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..244
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267800-rid25106413.denovo.910"
                     /serotype="O 26:H11"
                     /isolate="2201S31241"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-01"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            complement(87..>244)
                     /locus_tag="ACHOSC_RS28090"
                     /old_locus_tag="pgaptmp_005618"
     CDS             complement(87..>244)
                     /locus_tag="ACHOSC_RS28090"
                     /old_locus_tag="pgaptmp_005618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="T3SS effector protein Map"
                     /protein_id="HGU4551304.1"
                     /translation="VDPITRFNTQTRMIEQVSQEIFEQKFGGLKVSDIKALTQSTIAQ
                     HVQDTRL"
BASE COUNT           68 a           59 c           41 g           76 t
ORIGIN      
        1 tatagccttt tcttacatca gcaaagacta cgctatacac caggaagctt aacttatgcc
       61 caacacgcat atataacagg ttattcctac aaccgagtat cctgtacatg ctgtgcaatc
      121 gtgctctgag tcagagcttt gatatcacta actttcaaac caccaaattt ctgttcaaag
      181 atttcttgtg agacttgttc tatcatcctg gtctgggtgt taaagcgtgt aattggatcc
      241 accc
//
LOCUS       DAZKHF020000902          239 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2201S31241
            SAMN43267800-rid25106413.denovo.911, whole genome shotgun sequence.
ACCESSION   DAZKHF020000902 DAZKHF020000000
VERSION     DAZKHF020000902.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267800
            Sequence Read Archive: SRR30307263
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 239)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 239)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 239)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350581.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350581.2
            Genome Coverage       :: 151x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:21:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,652
            CDSs (total)                      :: 5,557
            Genes (coding)                    :: 5,320
            CDSs (with protein)               :: 5,320
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 237
            CDSs (without protein)            :: 237
            Pseudo Genes (ambiguous residues) :: 0 of 237
            Pseudo Genes (frameshifted)       :: 102 of 237
            Pseudo Genes (incomplete)         :: 130 of 237
            Pseudo Genes (internal stop)      :: 52 of 237
            Pseudo Genes (multiple problems)  :: 41 of 237
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..239
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267800-rid25106413.denovo.911"
                     /serotype="O 26:H11"
                     /isolate="2201S31241"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-01"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            complement(<1..219)
                     /locus_tag="ACHOSC_RS28095"
                     /old_locus_tag="pgaptmp_005619"
     CDS             complement(<1..219)
                     /locus_tag="ACHOSC_RS28095"
                     /old_locus_tag="pgaptmp_005619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000491542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="secretion protein EspS"
                     /protein_id="HGU4551305.1"
                     /translation="MFSIRSQLPISASVSIPAEQSQSISTIPGGRKIETVPEKEGLLV
                     FLGTKSVGEYALNILGQNVSRVTTGKKPY"
BASE COUNT           61 a           51 c           42 g           85 t
ORIGIN      
        1 ataaggtttt ttccctgttg tgactcttga aacattttga ccaagaatat taagagcata
       61 ttcacctacg gattttgttc ctaaaaacac aagcaatcct tctttctctg gcactgtctc
      121 tatttttctc cctccaggaa ttgtggagat ggattgagat tgttctgccg gaattgatac
      181 gctggcgctt ataggtagct gagatcttat actaaacata caacctcctt cttagtttc
//
LOCUS       DAZKHF020000903        16341 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2201S31241
            SAMN43267800-rid25106413.guided.084, whole genome shotgun sequence.
ACCESSION   DAZKHF020000903 DAZKHF020000000
VERSION     DAZKHF020000903.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267800
            Sequence Read Archive: SRR30307263
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 16341)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16341)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 16341)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350581.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350581.2
            Genome Coverage       :: 151x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:21:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,652
            CDSs (total)                      :: 5,557
            Genes (coding)                    :: 5,320
            CDSs (with protein)               :: 5,320
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 237
            CDSs (without protein)            :: 237
            Pseudo Genes (ambiguous residues) :: 0 of 237
            Pseudo Genes (frameshifted)       :: 102 of 237
            Pseudo Genes (incomplete)         :: 130 of 237
            Pseudo Genes (internal stop)      :: 52 of 237
            Pseudo Genes (multiple problems)  :: 41 of 237
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16341
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267800-rid25106413.guided.084"
                     /serotype="O 26:H11"
                     /isolate="2201S31241"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-01"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            402..1316
                     /gene="lafA"
                     /locus_tag="ACHOSC_RS28100"
                     /old_locus_tag="pgaptmp_005620"
     CDS             402..1316
                     /gene="lafA"
                     /locus_tag="ACHOSC_RS28100"
                     /old_locus_tag="pgaptmp_005620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001570969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lateral flagellin LafA"
                     /protein_id="HGU4551306.1"
                     /translation="MLSINTNNASMAAVNAISKSSSSLSTSMERLATGNRINSSADDA
                     AGKQIANRLTAQSSGMGVALSNINDATAMLQTADSMFDEMSDVLGRMKDLSTQAANGT
                     YSDDDLQAMQDEYDELGQQMSDMLQNTTYGGTNLFGVSGTSNTGTDGLFQSAVTFQVG
                     AESSDTMTVNISSQLNTLVTDLSAISNSFSADQADTTGTAGVSGGTELTASGSANQMI
                     TSISTAMDDVSQIQSKLGASINRLNDTANNLTSMQDNTEVAIGNIMDTDYATEASNMT
                     KQQVLMQTGITMLKQSNSMSSMVSSLLQ"
     gene            1523..2839
                     /gene="fliD"
                     /locus_tag="ACHOSC_RS28105"
                     /old_locus_tag="pgaptmp_005621"
     CDS             1523..2839
                     /gene="fliD"
                     /locus_tag="ACHOSC_RS28105"
                     /old_locus_tag="pgaptmp_005621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000609688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009288 - bacterial-type flagellum
                     [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar filament capping protein FliD"
                     /protein_id="HGU4551307.1"
                     /translation="MINPRTIAQEIAYADVATQAANLQEKQTELDAESSGLDSLSSAL
                     SDFQSAVDALNSDTDGPVTFAATSNNDSASVSANSQAQAGSYSFFVEQLAQGQQTTFS
                     MGDDAFSATGTFELTMGDSTMDIDLSAADQNGDGDGFIDASELVNAINDSDDNPGVSA
                     ALVKTDGTTTIMLTSDSTGAQSAFSVSVTGHDASNDSTSAPVATDVSSAQDAIIHLGS
                     ATGPEITNSSNTFDDVIPGVTMTFTEVSDSDSDLTTFNISEDSSASQEKVQTFVDAYN
                     TLIDTVDSLTTHGDDSTSAGVFAGDAGLSSLANQLDDIAHASYNGVSIVDYGITLDSH
                     GHLQIDSDQFNDEMAKNPDGLTSIFVGDNSMVAQMDDLINTYTDSSNGIITLRQQNID
                     DQMSKIQDEGDQLTDTYNANYDRYLEEYTNTLVEVYTMKASMAAFA"
     gene            2862..3254
                     /gene="fliS"
                     /locus_tag="ACHOSC_RS28110"
                     /old_locus_tag="pgaptmp_005622"
     CDS             2862..3254
                     /gene="fliS"
                     /locus_tag="ACHOSC_RS28110"
                     /old_locus_tag="pgaptmp_005622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000285320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0044780 - bacterial-type flagellum assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar export chaperone FliS"
                     /protein_id="HGU4551308.1"
                     /translation="MYQVQDGYSQYKEIDLAARTAAASPLELVLVLFSGLMDELERAK
                     SHIEGRRFEKKAQSINKCIDILNALTSSLEFETGGELVVNLSRLYDHCVYRLYEASGE
                     LSAEKIDEVMLILSNLREGWEGLSGKLG"
     gene            3259..3570
                     /locus_tag="ACHOSC_RS28115"
                     /old_locus_tag="pgaptmp_005623"
     CDS             3259..3570
                     /locus_tag="ACHOSC_RS28115"
                     /old_locus_tag="pgaptmp_005623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000414995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar protein FliT"
                     /protein_id="HGU4551309.1"
                     /translation="MEKQRRKLFALVEAMNEALDKQRWRRLPALHQQLIRQFHDYAAA
                     ETDAAQLDAVKARLYDAFNALIKRREQRAEVLKARMEQHQRHQEGVLAYSIVNLLSEK
                     S"
     gene            3567..4628
                     /locus_tag="ACHOSC_RS28120"
                     /old_locus_tag="pgaptmp_005624"
     CDS             3567..4628
                     /locus_tag="ACHOSC_RS28120"
                     /old_locus_tag="pgaptmp_005624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001067462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-length control protein FliK"
                     /protein_id="HGU4551310.1"
                     /translation="MNPTLLATLGTLAETASLKADILPPVSGENAPAFTLPKMAVAAV
                     AERVHSAKTSQQQATRPQENDPVAMQALMALLLPQPAAPHQDTPQPRNVATSPVIQQL
                     TKAVVQNAPQRQTQQQELTPLPPQLQELISQLPQAKPEQQARLATYASEDLHAITPTQ
                     PRVSTQPARPKSELTRVTARPQVERKTEKVPDSEPVIARAVLQVKTPELLSDHQEIVA
                     KPATLSMDELGEKLTTLLKDQIHFQLNKQQQISTIRLDPPSLGKLEIAVQLDNGKLMV
                     HIGANQSEVCRALQQFSDDLRQHLTAQNFMEVNVQVSSEGQSQQQQQSGHQQEEVSAA
                     LQLDDAPQFQQNESVLIKV"
     gene            4636..5103
                     /locus_tag="ACHOSC_RS28125"
                     /old_locus_tag="pgaptmp_005625"
     CDS             4636..5103
                     /locus_tag="ACHOSC_RS28125"
                     /old_locus_tag="pgaptmp_005625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001521900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009425 - bacterial-type flagellum basal
                     body [Evidence IEA];
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body-associated FliL family
                     protein"
                     /protein_id="HGU4551311.1"
                     /translation="MKKIVVAGVVSAVLALVVGAGAGWGVWHHYAGKGKPTAAAQTET
                     VETLDESKSVFVTLPETIVTLHDNNGADHYLSAELVMVVASDKEAEKIKHQEPLYQSI
                     AVECLTEMKFEDLRGMKISAIRKLISDALKKDLQRRKMSAPYKDLLVKKVVFQ"
     gene            5123..5839
                     /locus_tag="ACHOSC_RS28130"
                     /old_locus_tag="pgaptmp_005626"
     CDS             5123..5839
                     /locus_tag="ACHOSC_RS28130"
                     /old_locus_tag="pgaptmp_005626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000938732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FliA/WhiG family RNA polymerase sigma factor"
                     /protein_id="HGU4551312.1"
                     /translation="MLQDVFESEEFAAAPVLTPQQESHYLQAYLPLVRKVVRQLAPQC
                     NCIIDRQDMEQTALMGLLNAIRRYGLPDEGFAGYAVHRIRGAILDELRALDWRPRQLR
                     QKYHQMKDLIRETRKKLGHEPEWSELAVEGISHEDYLEYQQLEGAETLASLDELLNGE
                     GPGIPLVGRELEDQFVTQQMLQQALSQLSEKEQLILSMYYQHEMNLKEIALVLGLTEA
                     RICQLNKQIAQKIRDFVYPN"
     gene            5852..6715
                     /gene="motA"
                     /locus_tag="ACHOSC_RS28135"
                     /old_locus_tag="pgaptmp_005627"
     CDS             5852..6715
                     /gene="motA"
                     /locus_tag="ACHOSC_RS28135"
                     /old_locus_tag="pgaptmp_005627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001169515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor stator protein MotA"
                     /protein_id="HGU4551313.1"
                     /translation="MQKILGLLVVLGCVFGGYFEAGGQLVAIWQPAEMIIILGAGFGA
                     MIIGNPKHVLKEIAHQIKGVISKKQLGPEFQRQLLMCLYELLEMVQNGGLRMLDQHIE
                     QPEESTIFQKYPLVLTQKRLVTFIADNFRLMAMGKIDAHELEGILDQELDTAEESLLT
                     PSRSLQRTAEAMPGFGICAAVLGIIITMQSIDGSIALIGLKVAAALVGTFLGVFICYC
                     LMDPLANAMEQQARAEHSLLECVRTVLVAQAGGKPTLLAVDAGRKLLHLASKPTFANL
                     DAWVNAMLEQE"
     gene            6718..7641
                     /gene="lafU"
                     /locus_tag="ACHOSC_RS28140"
                     /old_locus_tag="pgaptmp_005628"
     CDS             6718..7641
                     /gene="lafU"
                     /locus_tag="ACHOSC_RS28140"
                     /old_locus_tag="pgaptmp_005628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001521902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative lateral flagellar export/assembly
                     protein LafU"
                     /protein_id="HGU4551314.1"
                     /translation="MRKTGNRRERGAKTTIVRRQIKKNHAGHHGGAWKVAFADFTLAM
                     MALFMTLWIVNSVSKSERESIIAALHGQSIFNGGGLSPLNKISPSHPPKPATVAVPEE
                     TEKKARDVNEKTALLKKKSATELGELATSINTIARDAHMEANLEMEIVPQGLRVLIKD
                     DQNRNMFERGSAQIMPFFKTLLVELAPVFDSLDNKIIITGHTDAMAYKNNIYNNWNLS
                     GDRALSARRVLEEAGMPEDKVMQVSAMADQMLLDSKNPQSAGNRRIEIMVLTKSASDT
                     LYQYFGQHGDKVVQPQVQKLDKQQVLSQRTR"
     gene            7712..8767
                     /gene="dinB"
                     /locus_tag="ACHOSC_RS28145"
                     /old_locus_tag="pgaptmp_005629"
     CDS             7712..8767
                     /gene="dinB"
                     /locus_tag="ACHOSC_RS28145"
                     /old_locus_tag="pgaptmp_005629"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003684 - damaged DNA binding [Evidence
                     IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase IV"
                     /protein_id="HGU4551315.1"
                     /translation="MRKIIHVDMDCFFAAVEMRDNPALRDIPIAIGGSRERRGVISTA
                     NYPARKFGVRSAMPTGMALKLCPHLTLLPGRFDAYKEASNHIREIFSRYTSRIEPLSL
                     DEAYLDVTDSVHCHGSATLIAQEIRQTIFSELQLTASAGVAPVKFLAKIASDMNKPNG
                     QFVITPAEVPAFLQTLPLAKIPGVGKVSAAKLEAMGLRTCGDVQKCDLVMLLKRFGKF
                     GRILWERSQGIDERDVNSERLRKSVGVERTMAEDIHHWSECEAIIERLYPELERRLAK
                     VKPDLLIARQGVKLKFDDFQQTTQEHVWPRLNKADLIATARKTWDERRGGRGVRLVGL
                     HVTLLDPQMERQLVLGL"
     gene            8764..9216
                     /gene="yafP"
                     /locus_tag="ACHOSC_RS28150"
                     /old_locus_tag="pgaptmp_005630"
     CDS             8764..9216
                     /gene="yafP"
                     /locus_tag="ACHOSC_RS28150"
                     /old_locus_tag="pgaptmp_005630"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HGU4551316.1"
                     /translation="MNNIQIRDYQPGDFQQLCAIFIRAVTMTASQHYSPQQIAAWAQI
                     DESRWKEKLAKSQVRVAVINAKLVGFITCVEHYIDMLFVDPEYTRRGVASALLKPLIK
                     SESELTVDASITAKPFFERYGFQTVKQQRVECRGEWFINFYMRYKQQH"
     gene            9462..10601
                     /locus_tag="ACHOSC_RS28155"
                     /old_locus_tag="pgaptmp_005631"
     CDS             9462..10601
                     /locus_tag="ACHOSC_RS28155"
                     /old_locus_tag="pgaptmp_005631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000521550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008452 - RNA ligase activity [Evidence
                     IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA ligase RtcB family protein"
                     /protein_id="HGU4551317.1"
                     /translation="MGNYIRPLSDAVFTIASDDLWIESLAIQQLHTTANLPNMQRVVG
                     MPDLHPGRGYPIGAAFFSVGRFYPALVGNDIGCGMALWQTDILARKYNADKFEKRLSD
                     LDDVAEESWLEENLPSAFAQHPWRSSLGSIGGGNHFAELQQVDQIINAELFALAGLDA
                     QHLQLLVHSGSRGVGQSILQRHIASFSHHGLPQGSDDALRYIAEHDDALAFACINRQM
                     IALRIMQQVKATGSPVLDVAHNFVSACQIGDQQGWLHRKGATPDDNGLVIIPGSRGDY
                     SWIVQPVANEKTLHSLAHGAGRKWGRTECKGRLAAKYTATQLSRTELGSRVICRDKQL
                     IFEEAPQAYKSAESVVQCLVQAGLIIPVARLRPVLTLKNSGGKKG"
     gene            10598..11212
                     /gene="prfH"
                     /locus_tag="ACHOSC_RS28160"
                     /old_locus_tag="pgaptmp_005632"
     CDS             10598..11212
                     /gene="prfH"
                     /locus_tag="ACHOSC_RS28160"
                     /old_locus_tag="pgaptmp_005632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000602105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003747 - translation release factor
                     activity [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor H"
                     /protein_id="HGU4551318.1"
                     /translation="MILLQLSSAQGPEECCLAVKKALDRLIKEAARQDVAVTVLETET
                     GHYSDTLRSALISLDGDNAWALSESWCGTIQWICPSPYRPHHGRKNWFLGIGRFTADE
                     QEQSDAIRYETLRSSGPGGQHVNKTDSAVRATHLASGISVKVQSERSQHANKRLARLL
                     IAWKLEQQQQENSAVLKSLRRMFHHQIERGNPRRTFTGMAFIEG"
     gene            complement(11269..12726)
                     /gene="pepD"
                     /locus_tag="ACHOSC_RS28165"
                     /old_locus_tag="pgaptmp_005633"
     CDS             complement(11269..12726)
                     /gene="pepD"
                     /locus_tag="ACHOSC_RS28165"
                     /old_locus_tag="pgaptmp_005633"
                     /EC_number="3.4.13.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytosol nonspecific dipeptidase"
                     /protein_id="HGU4551319.1"
                     /translation="MSELSQLSPQPLWDIFAKICSIPHPSYHEEQLAEYIVGWAKEKG
                     FHVERDQVGNILIRKPATAGMENRKPVVLQAHLDMVPQKNNDTVHDFTKDPIQPYIDG
                     EWVKARGTTLGADNGIGMASALAVLADENVVHGPLEVLLTMTEEAGMDGAFGLQSNWL
                     QADILINTDSEEEGEIYMGCAGGIDFTSNLHLDREAVPAGFETFKLTLKGLKGGHSGG
                     EIHVGLGNANKLLVRFLAGHAEELDLRLIDFNGGTLRNAIPREAFATIAVAADKVDVL
                     KSLVNTYQEILKNELAEKEKNLALLLDSVANDKAALIAKSRDTFIRLLNATPNGVIRN
                     SDVAKGVVETSLNVGVVTMTDNNVEIHCLIRSLIDSGKDYVVSMLDSLGKLAGAKTEA
                     KGAYPGWQPDANSPVMHLVRETYQRLFNKTPNIQIIHAGLECGLFKKPYPEMDMVSIG
                     PTITGPHSPDEQVHIESVGHYWTLLTELLKEIPAK"
     gene            12987..13445
                     /gene="gpt"
                     /locus_tag="ACHOSC_RS28170"
                     /old_locus_tag="pgaptmp_005634"
     CDS             12987..13445
                     /gene="gpt"
                     /locus_tag="ACHOSC_RS28170"
                     /old_locus_tag="pgaptmp_005634"
                     /EC_number="2.4.2.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000310 - xanthine
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine phosphoribosyltransferase"
                     /protein_id="HGU4551320.1"
                     /translation="MSEKYIVTWDMLQIHARKLASRLMPSEQWKGIIAVSRGGLVPGA
                     LLARELGIRHVDTVCISSYDHDNQRELKVLKRAEGDGEGFIVIDDLVDTGGTAVAIRE
                     MYPKAHFVTIFAKPAGRPLVDDYVVDIPQDTWIEQPWDMGVVFVPPISGR"
     gene            13537..14781
                     /gene="frsA"
                     /locus_tag="ACHOSC_RS28175"
                     /old_locus_tag="pgaptmp_005635"
     CDS             13537..14781
                     /gene="frsA"
                     /locus_tag="ACHOSC_RS28175"
                     /old_locus_tag="pgaptmp_005635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="esterase FrsA"
                     /protein_id="HGU4551321.1"
                     /translation="MTQANLSETLFKPRFKHPETSTLVRRFNHGAQPPVQSALDGKTI
                     PHWYRMINRLMWIWRGIDPREILDVQARIVMSDAERTDDDLYDTVIGYRGGNWIYEWA
                     TQAMVWQQKACAEEDPQLSGRHWLHAATLYNIAAYPHLKGDDLAEQAQALSNRAYEEA
                     AQRLPGTMRQMEFTVPGGAPITGFLHMPKGDGPFPTVLMCGGLDAMQTDYYSLYERYF
                     APRGIAMLTIDMPSVGFSSKWKLTQDSSLLHQHVLKALPNVPWVDHTRVAAFGFRFGA
                     NVAVRLAYLESPRLKAVACLGPVVHTLLSDFKCQQQVPEMYLDVLASRLGMHDASDDA
                     LRVELNRYSLKVQGLLGRRCPTPMLSGYWKNDPFSPEEDSRLITSSSADGKLLEIPFN
                     PVYRNFDKGLQEITGWIEKRLC"
     gene            14839..15240
                     /gene="crl"
                     /locus_tag="ACHOSC_RS28180"
                     /old_locus_tag="pgaptmp_005636"
     CDS             14839..15240
                     /gene="crl"
                     /locus_tag="ACHOSC_RS28180"
                     /old_locus_tag="pgaptmp_005636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0045893 - positive regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma factor-binding protein Crl"
                     /protein_id="HGU4551322.1"
                     /translation="MTLPSGHPKSRLIKKFTALGPYIREGKCEDNRFFFDCLAVCVNV
                     KPAPEVREFWGWWMELEAQESRFTYSYQFGLFDKAGDWKSVPVKDTEVVERLEHTLRE
                     FHEKLRELLTTLNLKLEPADDFRDEPVKLTA"
     gene            complement(15279..16334)
                     /gene="phoE"
                     /locus_tag="ACHOSC_RS28185"
                     /old_locus_tag="pgaptmp_005637"
     CDS             complement(15279..16334)
                     /gene="phoE"
                     /locus_tag="ACHOSC_RS28185"
                     /old_locus_tag="pgaptmp_005637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_process: GO:0034220 - monoatomic ion transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoporin PhoE"
                     /protein_id="HGU4551323.1"
                     /translation="MKKSTLALVVMGIVASASVQAAEIYNKDGNKLDVYGKVKAMHYM
                     SDNASKDGDQSYIRFGFKGETQINDQLTGYGRWEAEFAGNKAESDTAQQKTRLAFAGL
                     KYKDLGSFDYGRNLGALYDVEAWTDMFPEFGGDSSAQTDNFMTKRASGLATYRNTDFF
                     GVIDGLNLTLQYQGKNENRDVKKQNGDGFGTSLTYDFGGSDFAISGAYTNSDRTNEQN
                     LQSRGTGKRAEAWATGLKYDANNFYLATFYSETRKMTPITGGFANKTQNFEAVAQYQF
                     DFGLRPSLGYVLSKGKDIEGIGDEDLVNYIDVGATYYFNKNMSAFVDYKINQLDSDNK
                     LNINNDDIVAVGMTYQF"
BASE COUNT         4111 a         4057 c         4341 g         3832 t
ORIGIN      
        1 tttttctgcg tgataactaa tattttatgg taataggatt attcttaatc actatatggc
       61 atggaaatgt aacgaaatga caggactggt acaatagcca aatgtgtact atttgcaacg
      121 caatgtaaat tttggcatca gtacattgag aagaaaacgc gttaattgat ggtaatctta
      181 actatatcaa gcgtttgata aagacatatt ttgatacctg gccatgtggc agatattgct
      241 aataatcttt ttatctgaat ttaaactctg ctttttttag ctaaaagtgg catcagataa
      301 atcgcagtga aattttttta acggcggttt taagtttgct ccttaggtgt cgtttttaca
      361 gggtatcgaa acgaaaacga ttaatcccaa ggagcagtat catgttatct attaacacca
      421 ataacgcctc aatggccgcc gttaatgcaa tcagcaaaag cagctcttct ttgtctacat
      481 ctatggaacg cctggcgaca ggtaaccgga ttaactcttc tgccgatgac gccgcaggca
      541 aacaaattgc taaccgtctg acagcacagt ccagcggtat gggcgtggcg ctgagcaaca
      601 tcaatgacgc aaccgcgatg ctgcaaaccg ccgatagtat gttcgatgaa atgtctgatg
      661 tgctgggtcg tatgaaagac ctgtcaactc aggcagcgaa cggcacctac agcgacgacg
      721 atctgcaggc gatgcaggac gaatacgacg aactgggtca gcagatgtct gacatgctgc
      781 aaaacaccac ttacggcggc accaacctgt tcggtgtttc cggtaccagc aacaccggta
      841 ctgacggtct gttccagagc gcggtaactt tccaggtggg cgcggaatct tccgacacca
      901 tgaccgtgaa catctccagc caactgaaca cgctggtgac cgatctttct gctatcagca
      961 actcctttag cgcggatcag gcagacacca cgggtactgc aggtgtttct ggcggtactg
     1021 agctgaccgc gtctggctcg gctaaccaga tgatcaccag catctctacc gcgatggatg
     1081 acgtttcgca gatccagtcc aagctgggtg cgtctatcaa ccgtctgaac gacaccgcca
     1141 acaacctgac cagtatgcag gacaacaccg aagtcgctat cggtaacatt atggataccg
     1201 actacgcgac ggaagcgtcc aatatgacca aacagcaggt gctgatgcag actggcatca
     1261 ccatgctgaa acagtccaac agcatgtcca gcatggtttc cagcctgctg cagtaatggc
     1321 ggacttgccg gatgcggcgt aaacgcctta ttcggactac gaacaacacc gcctgcgggc
     1381 ggtgttgtgc tttctgcggc aggctttttt ccgccagacc ggaaacgcca tttccgcctg
     1441 ttttttaatc tattgattat tcacataaaa aatattggca cgccttttgc caataacctg
     1501 acatcgttat ccggagtcat caatgatcaa cccaagaacc attgcgcaag aaatcgcgta
     1561 tgcggatgtc gccactcagg cggcaaactt gcaggagaag caaacagagc tggatgctga
     1621 aagcagcggc ctggattcgc tcagttcagc gttgagcgat tttcagagcg ccgttgacgc
     1681 gctgaacagc gataccgacg gcccggtgac gtttgccgcc accagcaata atgactcggc
     1741 gagcgtttcc gccaattccc aggcgcaggc gggaagctac tcatttttcg ttgagcaact
     1801 ggcacagggg cagcaaacca cgttcagtat gggcgacgac gccttttccg ccaccggcac
     1861 ttttgaactg acgatgggcg acagcaccat ggatatcgat ctgtcggcgg cagatcagaa
     1921 tggtgacggc gacggtttta tcgacgccag tgaactggtg aatgcgatta acgactccga
     1981 tgataatccg ggcgtgtcgg cggcgctggt aaaaaccgac ggcaccacaa cgattatgct
     2041 cacctcggat agcaccgggg cgcagagcgc gttttcggtg agcgtaacgg ggcatgatgc
     2101 cagcaacgat agcaccagcg cgcccgttgc cacggatgtt tcctccgctc aggacgcgat
     2161 tattcatctt ggcagcgcca cggggcctga gatcaccaac agcagcaata cgtttgatga
     2221 tgtgattccc ggtgtcacca tgactttcac cgaagtcagc gattctgaca gcgatctcac
     2281 tacctttaac atcagcgaag attccagcgc cagccaggag aaagtacaga cctttgtcga
     2341 tgcctataac accttgattg atacggttga ttcgctaacc acccacggtg atgacagtac
     2401 cagcgccggg gtatttgcag gcgacgccgg actcagttca ctggcgaacc agctcgatga
     2461 catcgcccat gccagctaca acggcgtgtc gattgttgac tatggcatca ccctcgattc
     2521 tcacggccac ttacagattg actccgacca gttcaacgac gaaatggcga aaaatcctga
     2581 cggtctgacc tctattttcg ttggcgacaa cagcatggtg gcgcagatgg acgaccttat
     2641 taacacctac acggactcca gcaacggcat catcaccctg cgccagcaga acattgacga
     2701 tcagatgagc aaaattcagg acgaaggcga tcagcttact gatacctata acgccaacta
     2761 cgaccgttat ctcgaggagt acaccaacac gctggtcgag gtgtacacca tgaaagccag
     2821 catggcggca ttcgcgtaac tcagaaggaa tatccctaaa gatgtaccaa gtacaggacg
     2881 gctattcgca atataaagag atcgatctcg cggcgcgtac tgcggcggca tcgcctctgg
     2941 aactggtgct ggtgctgttt tctggcctga tggacgaact ggaacgcgcc aaaagccata
     3001 ttgaagggcg gcgtttcgag aagaaagccc agagcatcaa taaatgcatc gacattctca
     3061 atgcgctcac cagttcgctg gagtttgaaa ctggcggcga gctggtggtc aacctttcgc
     3121 gactctatga ccactgcgtt taccgattgt atgaggccag cggcgaactg tcggcagaga
     3181 aaatcgatga agtgatgctg atcttaagca acctgcgcga aggctgggaa gggctttcgg
     3241 gcaagctggg ttaagacgat ggaaaaacaa cgcaggaaac ttttcgcact ggtcgaggcg
     3301 atgaatgaag ccctcgacaa gcaacgctgg cgacgccttc cggcactgca tcagcagttg
     3361 atacgccagt ttcacgatta cgcggcggcg gaaaccgatg cggcgcagtt ggatgcagtg
     3421 aaagcgcgac tgtatgacgc gtttaacgcc ctgattaaac ggcgcgagca gcgcgcagaa
     3481 gtgttgaaag cgcgtatgga acagcatcag cggcatcagg aaggcgtact ggcctactca
     3541 attgttaatt tactttcgga gaagtcatga atccgacatt gcttgccacg ctcggaacgc
     3601 ttgcggaaac tgcttcgttg aaggcggata ttttaccgcc ggtgagcggt gaaaacgcgc
     3661 ccgcgtttac cctgccgaaa atggcggtcg cggcggtggc ggagcgcgtt catagcgcaa
     3721 aaaccagtca gcaacaggcg acgcgcccgc aggagaacga tccggtggcg atgcaggcgc
     3781 taatggcgct gttacttccg caacctgccg cgccgcatca ggacacgccg cagccgcgaa
     3841 acgtcgcaac atcgccggtt atccagcaat tgacgaaagc ggtggtgcaa aacgcgccgc
     3901 aacgccagac gcaacagcag gaactcacgc cgttgccgcc gcagttgcag gaactgatca
     3961 gtcagttgcc gcaggcgaag cccgaacagc aggccagact ggcgacttac gctagtgaag
     4021 atttacatgc cattacgccg acgcagccgc gcgtctcaac acagccagct cgcccgaaat
     4081 ctgaactaac ccgtgtgacc gcgcgcccgc aggtcgagcg taaaacggaa aaagtgccgg
     4141 acagtgaacc ggttattgcg cgtgcggtgt tgcaggttaa gacgccggag ctgctcagcg
     4201 atcatcagga gattgtcgcc aaaccagcca cgctttcgat ggacgaactg ggcgaaaaac
     4261 tgacgacgct tttgaaagat cagatccact ttcagctcaa caaacaacag cagatctcca
     4321 ccatccgtct cgatccaccg tcgcttggca agctcgagat cgccgtacaa ctcgacaacg
     4381 gcaaactgat ggtgcacatc ggcgcgaacc aaagtgaagt ttgccgcgcg ttacagcagt
     4441 ttagcgacga tctccgccag catctgacgg cgcaaaattt tatggaggtg aacgtacagg
     4501 tttcctccga agggcagtcg cagcaacaac aacagtcggg ccatcagcag gaagaggtga
     4561 gtgccgcctt acagcttgat gatgcgcctc aatttcaaca gaacgaatcc gttttgatca
     4621 aagtgtaaag gcaatatgaa gaaaatcgtg gtggcgggcg tggtcagcgc cgttctggcg
     4681 ttggtggtgg gggccggagc tggatggggt gtctggcatc attatgccgg aaaaggaaaa
     4741 ccgactgccg ccgcgcagac ggaaacggtg gaaaccctgg atgaaagcaa aagcgtgttt
     4801 gtgacgttgc cggaaaccat tgtcacgctg catgacaaca acggcgcgga ccattatttg
     4861 tcggcggaat tagtgatggt ggtcgccagt gacaaagaag cggaaaaaat aaaacatcag
     4921 gagccgctgt atcaaagtat tgcggttgaa tgcctgacgg aaatgaaatt tgaagatttg
     4981 cgcggcatga aaatatctgc catccgcaaa ctcatttctg acgcgttaaa gaaagatctt
     5041 cagcgtcgca aaatgagcgc accgtataag gatttgctgg tgaaaaaagt ggtcttccag
     5101 taatcaccag gcggatataa acatgctaca ggatgtattc gagtcagaag aatttgctgc
     5161 cgcgccggta ttaaccccgc agcaggagag ccactacttg caggcctatt taccgttggt
     5221 gcgtaaagtg gtgcggcaac ttgccccgca gtgtaattgc attattgatc gtcaggatat
     5281 ggaacaaacg gcgttaatgg ggctgcttaa tgccatccgc cgctacgggc tgccggacga
     5341 aggttttgcg ggctatgccg tgcaccgtat tcgcggcgcg atcctggatg agctgcgcgc
     5401 tctcgactgg cgaccgcgcc agctgcgaca gaaatatcat cagatgaaag atctgattcg
     5461 cgaaacgcgc aaaaaactgg ggcacgaacc ggagtggtca gaactggccg tggaagggat
     5521 ctcacatgaa gattatctcg aatatcagca actggaaggg gcggaaacgc tcgccagcct
     5581 ggacgaactg ctgaacggcg aagggccagg cattccgctt gttggccggg agctggaaga
     5641 tcagtttgtc actcagcaaa tgctgcaaca ggcgctttca caattaagcg aaaaagagca
     5701 actgatcctc tccatgtatt accagcatga gatgaatctc aaagagattg cgctggtatt
     5761 agggctaacc gaggcccgaa tttgtcaact gaataaacag atcgcgcaga aaattcgtga
     5821 ttttgtctat ccgaattaaa gtgaagaaat catgcaaaaa atattaggtt tactggtggt
     5881 gctcggctgc gtatttggcg gttattttga agcgggcggg caattagtgg ctatctggca
     5941 accggcggag atgatcatta ttttgggtgc cggtttcggg gcgatgatta ttggtaaccc
     6001 gaaacatgtg ctgaaagaga ttgcccatca aattaaaggt gtgatcagta aaaaacagct
     6061 cgggccggag tttcagcgtc agctattaat gtgcctgtat gagttgctcg aaatggtgca
     6121 aaacggtggt ctgcgcatgc tggatcaaca tattgagcaa ccggaagaga gcaccatttt
     6181 ccagaaatac ccgctggtgt taacccagaa gcggctggtg acctttattg ccgataactt
     6241 tcgcctgatg gcgatgggta aaatcgacgc ccatgaactg gaaggcattc tcgatcagga
     6301 actggacact gcggaagagt cgctgctcac gccgtcgcgc tcgctgcaac gtaccgccga
     6361 agcgatgccg ggatttggta tttgtgcagc agtgctgggg atcatcatca ccatgcagtc
     6421 gattgacggc tctattgccc tgatcggcct gaaagtggcg gcagcgctgg tggggacgtt
     6481 tttaggcgtc tttatctgct actgcctgat ggacccgctc gccaacgcca tggaacaaca
     6541 ggcgcgggca gaacattctt tgctggaatg cgtgcgcact gtgctggtgg cgcaggcagg
     6601 cggcaaaccg acgctgctgg cggtggatgc cgggcgtaag ctgctgcatc tggcgtccaa
     6661 accgaccttc gccaacctcg acgcgtgggt gaatgcgatg ctggagcaag aataagcatg
     6721 agaaagacgg gcaaccgccg cgagcgtggg gcaaaaacca ccatcgtccg gcggcagatc
     6781 aaaaagaatc atgccgggca tcatggcggg gcgtggaagg tggcgttcgc cgactttacc
     6841 ctggcgatga tggcgctgtt tatgacgctg tggattgtca acagcgtcag taaatccgag
     6901 cgtgaaagca ttatcgccgc gctgcacggg cagtcgattt ttaacggtgg cgggctgtcg
     6961 ccgttaaata aaatcagccc gtctcatccg ccaaaacctg ccaccgtggc ggtgccggaa
     7021 gagacggaga aaaaagcgcg tgatgtgaat gagaaaacgg cgctgctgaa gaagaaatcc
     7081 gccaccgagc ttggcgagct ggcaaccagt atcaacacca ttgcccgtga tgcgcatatg
     7141 gaagcgaatc tggagatgga gattgttccc cagggattac gcgtactgat taaagacgac
     7201 cagaatcgca atatgttcga acgcggcagc gcgcagatta tgccgttctt taaaacgctg
     7261 ctggtggagc tcgcgccagt tttcgactcg ctcgataaca aaattattat taccgggcat
     7321 accgatgcga tggcttacaa aaacaatatc tacaacaact ggaacctttc gggtgaccgc
     7381 gcgctttcgg ctcgtcgggt gctggaagag gccggaatgc cggaagataa agtgatgcag
     7441 gtaagcgcaa tggcggacca gatgctgctg gattccaaaa atccgcaaag cgcgggcaac
     7501 cggcgcattg agattatggt gctgaccaaa agtgcgtccg atacgctgta tcaatacttt
     7561 ggtcagcatg gggataaagt ggtgcagccg caggtgcaaa agctggataa gcagcaggtg
     7621 ctttcgcagc gaacgcgtta aatgctgaat ctttacgcat ttctcaaacc ctgaaatcac
     7681 tgtatacttt accagtgttg agaggtgagt aatgcgtaaa atcattcatg tggatatgga
     7741 ctgctttttc gcagcggtgg agatgcgcga caatcccgcc ctgcgcgata tccctattgc
     7801 tattggcggc agccgcgaac gtcggggggt gatcagcacc gccaattatc ccgcgcgtaa
     7861 atttggcgta cgtagcgcta tgccgacagg gatggcgctc aaattatgcc cgcatctcac
     7921 cttgcttccg gggcgctttg acgcctacaa agaagcctca aatcatatcc gtgaaatctt
     7981 ctcgcgctac acctcgcgta ttgaaccgtt gtcactggat gaggcttatc tcgacgtcac
     8041 cgatagcgtc cattgccacg gttctgcgac cctcatcgcc caggaaatcc gccagacgat
     8101 tttcagcgag ctgcaactga cagcgtctgc gggcgtggca ccagtaaagt ttctcgccaa
     8161 aatcgcctcc gacatgaata aacccaacgg tcagtttgtg attacgccgg cagaagttcc
     8221 ggcattttta caaaccttac cgctggcaaa aatccccggc gtcggcaaag tctcggcggc
     8281 aaaactggaa gcgatggggc tgcggacctg cggtgatgta caaaagtgtg atctggtgat
     8341 gctgcttaaa cgctttggca aatttggccg cattttgtgg gagcgtagtc aggggattga
     8401 cgagcgcgac gttaacagcg aacggttacg aaaatccgtc ggcgtggaac gcacgatggc
     8461 ggaagatatt catcactggt ctgaatgtga agcgattatc gagcggctgt atccggaact
     8521 tgaacgccgt ctggcaaagg taaaacctga tttactgatc gcccgtcagg gagtgaaatt
     8581 aaagtttgat gattttcagc aaaccactca ggagcacgtc tggccgcggc tgaataaagc
     8641 tgacttaatc gccaccgcgc gtaaaacctg ggatgaacgc cgcggcgggc gcggtgtgcg
     8701 actggtgggg ctgcatgtga cgttgcttga cccgcaaatg gaaagacaac tggtgctggg
     8761 attatgaata acatacaaat aagagactat cagcctggcg attttcagca actatgcgct
     8821 attttcatta gagcggttac gatgactgcc agtcagcatt attcaccaca acaaattgcc
     8881 gcctgggcgc agattgacga atctcgctgg aaggagaaac tcgcgaaatc acaagtgcgg
     8941 gttgcagtca ttaatgcaaa actggtcggc tttattacct gcgttgaaca ctatatcgat
     9001 atgttatttg ttgaccctga atacacccgc cgtggggttg ccagcgcttt gttaaaacct
     9061 ttgattaagt ctgaatccga acttacggtg gacgcaagca taaccgcaaa accctttttt
     9121 gaacgttatg gttttcagac agttaagcag caacgcgttg aatgtcgggg agagtggttt
     9181 attaattttt atatgcgtta taaacagcaa cattaaatcc agcttgcaat gaaaataacg
     9241 caagcctagt atgtgcgcgt tttcttattc cacaaactgc aaggaggtaa atcatgacaa
     9301 atcctttatc aatgactctt tgcagacctt tccaggatta attctttttt tcttgccctg
     9361 gattcgtctg ccatttcctg attttttaat tgttactctc aataattcaa gtcgcaacac
     9421 atgcaacttg aagtatgacg agtctattta cggaatggta tatgggcaat tatattcgtc
     9481 ccttatccga tgcggtattt accatcgcat ctgatgacct gtggatcgag agtttagcga
     9541 tccaacaatt acacaccacg gcaaatttac ccaacatgca gcgcgtagtg gggatgccag
     9601 atttacatcc cggacgcggc tatccgattg gcgcagcgtt cttctctgtt ggtcgttttt
     9661 atccggcact ggtcggcaat gatatcggct gcggtatggc gctatggcaa acagatattc
     9721 tcgctcgcaa atacaacgcc gataagtttg aaaagcgatt atcagatctg gatgacgttg
     9781 ctgaagaaag ctggctggag gaaaacctgc cgtcagcatt tgcacagcat ccgtggcgca
     9841 gctcgcttgg ctctatcggt ggcggcaatc atttcgctga actgcaacaa gttgatcaaa
     9901 tcatcaacgc tgaactgttt gcactggcag gtctggatgc acaacatctg caactgttgg
     9961 ttcatagcgg ctcgcggggg gtggggcagt ctattttaca gcggcatatt gcctcgttct
    10021 cgcatcatgg tttgcctcaa ggcagtgacg acgcgctacg gtatattgcg gaacatgacg
    10081 atgcgctggc gtttgcgtgt attaatcgcc agatgatcgc tttgcgcata atgcaacaag
    10141 ttaaggccac gggaagtccg gttctggatg tggcgcataa ctttgttagc gcgtgtcaaa
    10201 tcggcgatca acagggctgg ttgcatcgta aaggtgccac gccggatgac aacggtctgg
    10261 tgattattcc cggttcacgt ggtgattact cctggattgt acagcctgtt gcgaacgaaa
    10321 aaacgctcca ctcgctggcg catggggcag ggcgtaaatg ggggcgcacc gagtgtaaag
    10381 ggcgtctggc agcgaaatac acggcgacgc aactctcgcg tactgaactt ggcagtcggg
    10441 taatttgtcg cgataaacaa ctcatctttg aagaagcgcc acaagcctat aaatcggctg
    10501 aaagcgtggt gcaatgtctg gtgcaggccg gtttaattat tcctgtcgcg cggctgcgtc
    10561 cggtgctaac gctcaaaaac agtggaggga aaaaaggatg atcttgctac aactctcctc
    10621 agcgcagggg ccggaagaat gttgtctcgc agtgaaaaag gcgctggaca ggctaattaa
    10681 agaagctgcc cgacaagacg tcgcggtaac ggtgctggaa acagaaacgg gccactactc
    10741 tgacacgctg cgttcggcgc tgatttctct ggatggcgat aacgcatggg cattaagcga
    10801 aagctggtgt ggcactattc agtggatttg tccgagtccg tatcggcctc atcatgggcg
    10861 caaaaactgg tttctgggca ttgggcgttt taccgctgat gagcaggaac aatcggatgc
    10921 aatccgttat gagacgttgc gttcgtcggg gccgggcggt caacatgtca ataaaaccga
    10981 ctcggcggta cgcgccacgc atttggcatc cggtattagc gtgaaggttc agtcagagcg
    11041 tagtcagcat gctaacaagc ggctggcacg attgctgatt gcctggaagc tggagcagca
    11101 gcaacaggaa aatagcgcgg tgctgaaatc gctgcggcga atgttccatc accagattga
    11161 acgtggcaac ccgagacgga catttacagg gatggctttt atcgaaggat aatgaaggat
    11221 gaaactgccg gaaggcgatt aaacgccatc cggcagcaaa tcaaataatt acttcgccgg
    11281 aatttctttc agcagttcag tcagcagcgt ccagtaatga cctacgcttt cgatgtgaac
    11341 ttgctcatcc ggagagtgtg gaccggtgat agttggcccg atagaaacca tgtccatttc
    11401 cggatacggt tttttgaaca gaccacattc caggcccgcg tggataatct ggatgttcgg
    11461 tgttttgttg aacagacgct gataggtttc acgtaccaga tgcatcaccg gagaattagc
    11521 gtccggctgc cagccaggat atgcgccttt cgcttcggtt ttcgcgccag ctagtttacc
    11581 cagcgaatcc agcatgctca ccacgtagtc tttaccgctg tcgatcagtg aacggatcag
    11641 gcagtgaatt tctacgttat tgtcagtcat ggtcaccaca ccgacgttca gggaggtttc
    11701 gaccacacct tttgccacgt cggaattgcg gatcacaccg ttcggggtgg cgttcagcag
    11761 acgaataaag gtatcgcgag atttcgcaat cagggcagct ttatcgttcg ctacagagtc
    11821 cagcaacaag gccagatttt tctctttctc tgccagctcg tttttcagga tctcctgata
    11881 ggtattcacc agagatttca ggacgtcgac tttatcagct gcgacagcaa tggtcgcaaa
    11941 ggcttcacgc gggatggcgt tacgcagtgt gccgccgttg aaatcgataa ggcgcagatc
    12001 cagttcttcc gcatgacccg ccaggaagcg caccagtagt ttgttggcat tacccaggcc
    12061 aacgtggatt tcaccgccgg agtgaccgcc tttcagacct tttaaggtta acttgaaggt
    12121 ttcaaaacca gctggaaccg cttcacgatc taaatgcagg ttggaggtga agtcgatacc
    12181 ccccgcacaa cccatgtaga tttcaccttc ttcttcggag tcggtgttaa tcagaatatc
    12241 agcctgcaac cagttgctct gtaagccgaa cgcaccgtcc ataccggctt cttcggtcat
    12301 ggtcagcagc acttccagcg ggccgtgaac cacgttttcg tcagccagaa ccgccagcgc
    12361 agacgccata ccaatgccgt tatctgcacc cagcgtggtg ccgcgcgctt taacccattc
    12421 gccatcaata taaggctgga taggatcttt cgtgaagtca tgcacggtgt cgttattttt
    12481 ctgcggcacc atatcgaggt gggcctgtaa gacgaccggt ttacgatttt ccatacctgc
    12541 ggtagcaggt ttacgaatca ggatattacc tacctgatcg cgttcgacat ggaaaccttt
    12601 ctcttttgcc caaccaacaa tgtattcagc gagttgctct tcatgatagg acgggtgagg
    12661 aatagaacag attttggcaa aaatatccca cagcggctgt ggagataatt gagacagttc
    12721 agacacgtta agtctccttg tcgatcaccc gcaaaacagt attgcaggtc acagggttag
    12781 cagaaaatgt tgtcaacaca agacaggctt gcgagatatg tttgagaata ccactttatc
    12841 ccgcgtcagg gagaggcagt gcgtaaaaag acgcggactc atgtgaaata ctggttttta
    12901 gtgcgccaga tctctataat ctcgcgcaac ctattttccc ctcgaacact ttttaagccg
    12961 tagataaaca ggctgggaca cttcacatga gcgaaaaata catcgtcacc tgggacatgt
    13021 tgcagatcca tgcacgtaaa ctcgcaagcc gactgatgcc ttctgaacaa tggaaaggca
    13081 ttattgccgt aagccgtggc ggtctggtac cgggtgcgtt actggcgcgt gaactgggta
    13141 ttcgtcatgt cgataccgtt tgtatttcca gctacgatca cgacaaccag cgcgagctta
    13201 aagtgctgaa acgcgcagaa ggcgatggcg aaggcttcat cgttattgat gacctggtgg
    13261 ataccggtgg tactgctgtt gcgattcgtg aaatgtatcc aaaagcgcac tttgtcacca
    13321 tcttcgcaaa accggctggt cgtccgctgg ttgatgacta tgttgttgat atcccgcaag
    13381 atacctggat cgaacagccg tgggatatgg gcgtcgtatt cgtcccgcca atctccggtc
    13441 gctaatcttt tcaacgcctg gcactgccgg gcgttgttct ttttaacttc aggcaggtta
    13501 caatagtttc cagtaagtat tctggaggct gcatccatga cacaggcaaa cctgagcgaa
    13561 accctgttca aaccccgctt taaacatcct gaaacctcga cgctagtccg ccgctttaat
    13621 cacggcgcac aaccgcctgt gcagtcggct cttgatggta aaaccatccc tcactggtat
    13681 cgcatgatta accgtctgat gtggatctgg cgcggtattg acccacgtga aatcctcgac
    13741 gtccaggcac gtattgtgat gagcgatgct gaacgtaccg acgatgattt atacgatact
    13801 gtgattggct accgtggtgg caactggatt tatgagtggg ccacccaggc gatggtgtgg
    13861 caacaaaaag cctgtgcgga agaagatccg caactcagtg gtcgacactg gctgcatgcg
    13921 gctacgttgt acaacattgc cgcctatcct catctgaaag gagatgacct ggccgagcaa
    13981 gcgcaggctt tatcaaaccg cgcctatgaa gaggccgccc agcgtctgcc gggcacgatg
    14041 cggcagatgg agtttaccgt acccggcggt gcgcccatca ccggcttttt gcatatgccg
    14101 aaaggcgatg gcccgttccc gacagtatta atgtgtggtg gtctggatgc gatgcagacg
    14161 gactattaca gcctgtatga acgttatttt gcgccgcgcg gcattgcgat gctgactatt
    14221 gatatgccat cggtgggctt ttcttcaaaa tggaagctca cccaggactc cagcctgttg
    14281 catcagcacg tcttaaaggc gctgcctaac gtaccgtggg tggatcacac tcgcgtcgcg
    14341 gcctttggtt tccgtttcgg cgctaacgtt gccgtgcgtc tggcatatct tgaatcgccg
    14401 cgtctgaaag cggttgcctg tcttggtccg gtagttcata ccctgttgag tgattttaag
    14461 tgccagcaac aggtgccgga aatgtatctt gacgttctgg cgagtcgttt ggggatgcat
    14521 gatgcttccg atgacgcgtt acgcgtggag ctgaatcgct attcattaaa agtgcaagga
    14581 ttgctggggc gtcgctgccc aacgccaatg ttatcaggct actggaagaa cgatccgttc
    14641 agcccggaag aggactcacg cttaatcacc tcatcatctg ctgacggtaa attattagag
    14701 atcccattta acccggtgta tcggaatttt gacaaaggtc ttcaggaaat caccggctgg
    14761 atcgaaaaac gcttgtgtta aaaatttgct aaattttgcc aatttggtaa aacagttgca
    14821 tcacaacagg agatagcaat gacgttaccg agtggacacc cgaagagcag attgatcaaa
    14881 aaatttaccg cactaggccc gtatattcgt gaaggtaagt gcgaagataa tcgattcttt
    14941 ttcgattgtc tggctgtatg cgtcaacgtg aaaccggcac cggaagtgcg tgagttctgg
    15001 ggctggtgga tggagcttga agcgcaggaa tcccgtttta cctacagtta ccagtttggt
    15061 ctgttcgata aagcaggcga ctggaagagt gttccggtaa aagacactga agtggttgaa
    15121 cgactggagc acaccctgcg tgagtttcac gagaagctgc gtgaactgct gacgacgctg
    15181 aatctgaagc tggaaccggc ggatgatttt cgtgacgagc cggtgaagtt aacggcgtga
    15241 gtgaaatgtg ccggatgcat cacatccggc aataatcatt aaaactgata ggtcatgcca
    15301 accgcgacaa tatcatcatt attaatattc aatttgttat cgctatccag ttggttgatt
    15361 ttataatcaa caaacgctga catatttttg ttgaaataat atgtagcccc gacatcgata
    15421 taattgacca gatcttcatc accgatacct tcaatatctt tccctttcga taagacataa
    15481 cccagcgatg gacgcagacc aaagtcaaac tggtattgag cgaccgcttc aaagttctgt
    15541 gtcttattgg caaagccgcc agttattggc gtcattttgc gtgtttcaga ataaaaagtt
    15601 gccagataaa aattattggc atcgtatttc agacctgtag cccatgcttc tgcacgcttg
    15661 cctgtgccac ggctttgcag gttctgctcg ttggtgcgat ctgagttggt ataggcccca
    15721 ctaatggcga aatcgctgcc gccaaagtca tatgtcaatg acgtgccgaa gccatcgccg
    15781 ttttgctttt taacgtcgcg gttttcgttt ttcccttgat attgcagggt taagttcagg
    15841 ccatcgataa cgccgaagaa gtcggtgttc cgatacgtcg ccagaccgct ggcgcgtttg
    15901 gtcataaagt tgtcggtctg cgccgaggaa tcgccaccaa attccgggaa catatcggtc
    15961 caggcttcca cgtcatacaa cgcgcccagg ttacgaccat aatcgaaaga acccaaatct
    16021 ttatatttca acccggcaaa agcgagacgc gttttttgct gtgcggtatc actctccgct
    16081 ttatttccgg caaactccgc ttcccaccga ccataaccag tcagttgatc gttaatttgt
    16141 gtttcacctt taaaaccaaa acggatataa ctctggtcgc catctttact ggcgttatca
    16201 ctcatataat gcatggcttt aactttgcca tagacatcca gtttattacc gtctttatta
    16261 tatatttctg cagcctgtac agatgcagat gccacaatgc ccatcaccac taatgccaga
    16321 gtgctctttt tcattttcat t
//
LOCUS       DAZKHF020000904         8085 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2201S31241
            SAMN43267800-rid25106413.guided.197, whole genome shotgun sequence.
ACCESSION   DAZKHF020000904 DAZKHF020000000
VERSION     DAZKHF020000904.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267800
            Sequence Read Archive: SRR30307263
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 8085)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8085)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 8085)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350581.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350581.2
            Genome Coverage       :: 151x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:21:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,652
            CDSs (total)                      :: 5,557
            Genes (coding)                    :: 5,320
            CDSs (with protein)               :: 5,320
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 237
            CDSs (without protein)            :: 237
            Pseudo Genes (ambiguous residues) :: 0 of 237
            Pseudo Genes (frameshifted)       :: 102 of 237
            Pseudo Genes (incomplete)         :: 130 of 237
            Pseudo Genes (internal stop)      :: 52 of 237
            Pseudo Genes (multiple problems)  :: 41 of 237
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8085
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267800-rid25106413.guided.197"
                     /serotype="O 26:H11"
                     /isolate="2201S31241"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-01"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            complement(<1..703)
                     /gene="yjeM"
                     /locus_tag="ACHOSC_RS28190"
                     /old_locus_tag="pgaptmp_005638"
     CDS             complement(<1..703)
                     /gene="yjeM"
                     /locus_tag="ACHOSC_RS28190"
                     /old_locus_tag="pgaptmp_005638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418580.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate/gamma-aminobutyrate family transporter
                     YjeM"
                     /protein_id="HGU4551324.1"
                     /translation="MPHTIKKMSLIGLILMIFTSVFGFANSPSAYYLMGYSAIPFYIF
                     SALLFFIPFALMMAEMGAAYRKEEGGIYSWMNNSVGPRFAFIGTFMWFSSYIIWMVST
                     SAKVWVPFSTFLYGSDMTQHWRIAGLEPTQVVGLLAVAWMILVTVVASKGINKIARIT
                     AVGGIAVMCLNLVLLLVSITILLLNGGHFAQDINFLASPNPGYQSGLAMLSFVVFAIF
                     AYGGIEAVGGLVDKTE"
     gene            complement(922..1899)
                     /gene="epmA"
                     /locus_tag="ACHOSC_RS28195"
                     /old_locus_tag="pgaptmp_005639"
     CDS             complement(922..1899)
                     /gene="epmA"
                     /locus_tag="ACHOSC_RS28195"
                     /old_locus_tag="pgaptmp_005639"
                     /EC_number="6.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000004771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P--(R)-beta-lysine ligase"
                     /protein_id="HGU4551325.1"
                     /translation="MSETASWQPSASIPNLLKRAAIMAEIRRFFADRGVLEVETPCMS
                     QATVTDIHLVPFETRFVGPGHSQGMNLWLMTSPEYHMKRLLVAGCGPVFQLCRSFRNE
                     EMGRYHNPEFTMLEWYRPHYDMYRLMNEVDDLLQQVLDCPAAESLSYQQAFLRYLEID
                     PLSADKTQLREVAAKLDLSNVADTEEDRDTLLQLLFTFGVEPNIGKEKPTFVYHFPAS
                     QASLAQISTEDHRVAERFEVYYKGIELANGFHELTDAREQQQRFEQDNRKRAARGLPQ
                     HPIDQNLIEALKVGMPDCSGVALGVDRLVMLALGAETLAEVIAFSVDRA"
     gene            2224..4032
                     /gene="frdA"
                     /locus_tag="ACHOSC_RS28200"
                     /old_locus_tag="pgaptmp_005640"
     CDS             2224..4032
                     /gene="frdA"
                     /locus_tag="ACHOSC_RS28200"
                     /old_locus_tag="pgaptmp_005640"
                     /EC_number="1.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0009061 - anaerobic respiration [Evidence
                     IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate reductase (quinol) flavoprotein
                     subunit"
                     /protein_id="HGU4551326.1"
                     /translation="MQTFQADLAIVGAGGAGLRAAIAAAQANPNAKIALISKVYPMRS
                     HTVAAEGGSAAVAQDHDSFEYHFHDTVAGGDWLCEQDVVDYFVHHCPTEMTQLELWGC
                     PWSRRPDGSVNVRRFGGMKIERTWFAADKTGFHMLHTLFQTSLQFPQIQRFDEHFVLD
                     ILVDDGHVRGVVAMNMMEGTLVQIRANAVVMATGGAGRVYRYNTNGGIVTGDGMGMAL
                     SHGVPLRDMEFVQYHPTGLPGSGILMTEGCRGEGGILVNKNGYRYLQDYGMGPETPLG
                     EPKNKYMELGPRDKVSQAFWHEWRKGNTISTPRGDVVYLDLRHLGEKKLHERLPFICE
                     LAKAYVGVDPVKEPIPVRPTAHYTMGGIETDQNCETRIKGLFAVGECSSVGLHGANRL
                     GSNSLAELVVFGRLAGEQATERAATAGNGNEAAIEAQAAGVEQRLKDLVNQDGGENWA
                     KIRDEMGLAMEEGCGIYRTPELMQKTIDKLAELQERFKRVRITDTSSVFNTDLLYTIE
                     LGHGLNVAECMAHSAMARKESRGAHQRLDEGCTERDDVNFLKHTLAFRDADGTTRLEY
                     SDVKITTLPPAKRVYGGEADAADKAEAANKKEKANG"
     gene            4025..4759
                     /gene="frdB"
                     /locus_tag="ACHOSC_RS28205"
                     /old_locus_tag="pgaptmp_005641"
     CDS             4025..4759
                     /gene="frdB"
                     /locus_tag="ACHOSC_RS28205"
                     /old_locus_tag="pgaptmp_005641"
                     /EC_number="1.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020882957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate reductase iron-sulfur protein"
                     /protein_id="HGU4551327.1"
                     /translation="MAEMKNLKIEVVRYNPEVDTAPHSAFYEVPYDATTSLLDALGYI
                     KDNLAPDLSYRWSCRMAICGSCGMMVNNVPKLACKTFLRDYTDGMKVEALANFPIERD
                     LVVDMTHFIESLEAIKPYIIGNSRTADQGTNIQTPAQMAKYHQFSGCINCGLCYAACP
                     QFGLNPEFIGPAAITLAHRYNEDSRDHGKKERMAQLNSQNGVWSCTFVGYCSEVCPKH
                     VDPAAAIQQGKVESSKDFLIATLKPR"
     gene            4770..5165
                     /gene="frdC"
                     /locus_tag="ACHOSC_RS28210"
                     /old_locus_tag="pgaptmp_005642"
     CDS             4770..5165
                     /gene="frdC"
                     /locus_tag="ACHOSC_RS28210"
                     /old_locus_tag="pgaptmp_005642"
                     /EC_number="1.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate reductase subunit FrdC"
                     /protein_id="HGU4551328.1"
                     /translation="MTTKRKPYVRPMTSTWWKKLPFYRFYMLREGTAVPAVWFSIELI
                     FGLFALKNGPEAWAGFVDFLQNPVIVIINLITLAAALLHTKTWFELAPKAANIIVKDE
                     KMGPEPIIKSLWAVTVVATIVILFVALYW"
     gene            5176..5535
                     /gene="frdD"
                     /locus_tag="ACHOSC_RS28215"
                     /old_locus_tag="pgaptmp_005643"
     CDS             5176..5535
                     /gene="frdD"
                     /locus_tag="ACHOSC_RS28215"
                     /old_locus_tag="pgaptmp_005643"
                     /EC_number="1.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate reductase subunit FrdD"
                     /protein_id="HGU4551329.1"
                     /translation="MINPNPKRSDEPVFWGLFGAGGMWSAIIAPVMILLVGILLPLGL
                     FPGDALSYERVLAFAQSFIGRVFLFLMIVLPLWCGLHRMHHAMHDLKIHVPAGKWVFY
                     GLAAILTVVTLIGIVTI"
     gene            5598..6731
                     /locus_tag="ACHOSC_RS28220"
                     /old_locus_tag="pgaptmp_005644"
     CDS             5598..6731
                     /locus_tag="ACHOSC_RS28220"
                     /old_locus_tag="pgaptmp_005644"
                     /EC_number="3.5.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001336292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008800 - beta-lactamase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BlaEC family class C beta-lactamase"
                     /protein_id="HGU4551330.1"
                     /translation="MFKTTLCALLITASCSTFAAPQQINDIVHRTITPLIEQQKIPGM
                     AVAVIYQGKPYYFTWGYADIAKKQPVTQQTLFELGSVSKTFTGVLGGDAIARGEIKLS
                     DPTTKYWPELTAKQWNGITLLHLATYTAGGLPLQVPDEVKSSSDLLRFYQNWQPAWAP
                     GTQRLYANSSIGLFGALAVKPSGLSFEQAMQTRVFQPLKLNHTWINVPPAEEKNYAWG
                     YREGKAVHVSPGALDAETYGVKSTIEDMACWVRSNMNPRDINDKTLQQGIQLAQSRYW
                     QTGDMYQGLGWEMLDWPVNPDSIINGSGNKIALAAHPVKAITPPTPAVRASWVHKTGA
                     TGGFGSYVAFIPEKELGIVMLANKNYPNPARVAAAWQILNALQ"
     gene            6820..7353
                     /gene="blc"
                     /locus_tag="ACHOSC_RS28225"
                     /old_locus_tag="pgaptmp_005645"
     CDS             6820..7353
                     /gene="blc"
                     /locus_tag="ACHOSC_RS28225"
                     /old_locus_tag="pgaptmp_005645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipocalin Blc"
                     /protein_id="HGU4551331.1"
                     /translation="MRLLPLVAAATAAFLVVACSSPTPPRGVTVVNNFDAKRYLGTWY
                     EIARFDHRFERGLEKVTATYSLRDDGGLNVINKGYNPDRGMWQQSEGKAYFTGAPTRA
                     ALKVSFFGPFYGGYNVIALDREYRHALVCGPDRDYLWILSRTPTISDEVKQEMLAVAT
                     REGFDVSKFIWVQQPGS"
     gene            complement(7350..7667)
                     /gene="sugE"
                     /locus_tag="ACHOSC_RS28230"
                     /old_locus_tag="pgaptmp_005646"
     CDS             complement(7350..7667)
                     /gene="sugE"
                     /locus_tag="ACHOSC_RS28230"
                     /old_locus_tag="pgaptmp_005646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418572.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="quaternary ammonium compound efflux SMR
                     transporter SugE"
                     /protein_id="HGU4551332.1"
                     /translation="MSWIILVIAGLLEVVWAVGLKYTHGFSRLTPSVITVTAMIVSMA
                     LLAWAMKSLPVGTAYAVWTGIGAVGAAITGIVLLGESANPMRLASLALIVLGIIGLKL
                     STH"
     gene            complement(7849..7995)
                     /gene="ecnB"
                     /locus_tag="ACHOSC_RS28235"
                     /old_locus_tag="pgaptmp_005647"
     CDS             complement(7849..7995)
                     /gene="ecnB"
                     /locus_tag="ACHOSC_RS28235"
                     /old_locus_tag="pgaptmp_005647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein toxin entericidin B"
                     /protein_id="HGU4551333.1"
                     /translation="MVKKTIAAIFSVLVLSTVLTACNTTRGVGEDISDGGNAISGAAT
                     KAQQ"
BASE COUNT         2092 a         2098 c         2041 g         1854 t
ORIGIN      
        1 tttccgtttt atcgaccaga ccaccaaccg cttcaattcc gccataggca aaaatagcaa
       61 ataccacaaa cgatagcatt gccagaccgg actgataacc tgggttcggt gatgcaagga
      121 aattaatatc ctgcgcgaaa tgcccaccat ttaataacaa aatagtaatg cttactaaca
      181 gcaatactaa attcagacac attactgcaa taccgcccac cgcagtaatg cgggcaattt
      241 tattaatccc ctttgaagca acgacggtga ccagaatcat ccatgccact gccagcagac
      301 caaccacctg cgtaggctcc agtccagcaa tacgccagtg ctgggtcatg tcgctaccat
      361 agaggaatgt tgagaacggt acccaaactt tcgcggaggt actcaccatc cagatgatat
      421 aagaggaaaa ccacataaac gtaccaatga aggcaaaacg tggtccgaca ctattattca
      481 tccaggaata gataccgcct tcttctttgc gataagcagc tcccatttca gccatcatta
      541 aggcgaatgg aataaagaat aacaatgcag aaaatatata aaagggaatc gcactataac
      601 ccattaagta ataagccgat gggctattgg caaatccaaa tacggaagta aagatcatca
      661 atatgagtcc tatcagactc atctttttta tcgtgtgagg catgaaacca tccttcattt
      721 cagcgcgtgt ataacacgtg cccactgaac tgcactgcgc aacattgagc aggcagcgta
      781 aaaaccaata cagaattgat acctggatga tatcaaatat ccagtcttaa attgtggcct
      841 ttaacggaaa aatgaaaaca tatgctacag aatggcgctt atcacgccat tcttcgctgt
      901 taattcagta atttttcaga attatgcccg gtcaacgcta aaggcgatga cttcagccag
      961 tgtctccgcg cccagcgcca acatcaccag acgatcaaca cctaatgcca cgccggaaca
     1021 gtcaggcata ccgactttca aggcttcaat cagattctgg tcaatggggt gctgcggcaa
     1081 accgcgcgcc gcgcgcttac ggttatcttg ttcaaagcgt tgttgctgct cacgggcatc
     1141 cgtcaattca tggaaaccat tcgccagctc aataccttta taataaacct caaagcgttc
     1201 agcgacccga tgatcttcgg tactgatttg cgccagtgat gcctggctgg ctggaaagtg
     1261 gtacacaaag gtcggttttt ctttgccaat atttggctct acgccaaagg taaacagcaa
     1321 ttgtagcagc gtgtcgcggt cttcttcggt atcagcaaca ttgctcaaat ccagtttcgc
     1381 tgcgacttcc cgcagttgcg ttttgtcggc agagagcggg tcaatttcca gataacgcaa
     1441 gaaagcttgt tgataagaaa ggctttctgc tgccgggcag tccagcacct gttgtaagag
     1501 atcgtccacc tcgttcatca accggtacat atcatagtgc ggtcgatacc actccagcat
     1561 agtgaactca gggttgtgat aacgccccat ctcttcatta cggaagctgc ggcacagctg
     1621 gaataccggc ccacaaccgg caaccagcag gcgtttcata tggtattccg ggctggtcat
     1681 taaccagaga ttcatcccct gcgaatgccc ggggccaacg aaacgtgtct caaacgggac
     1741 caaatgaata tcggttaccg tcgcctggct catacaaggc gtctccacct ccagcactcc
     1801 acgatcggca aagaaacgac ggatctccgc cataatcgcc gcgcgtttta ataagttagg
     1861 aatggatgcg ctcggctgcc aggatgccgt ttcgctcata gttaaatctc cagtttttga
     1921 caagggcacg aagtctactc gcaacgcgac ggcgagacaa attttacgca ggaatcaaac
     1981 agcggtgggc agtgactaaa aaaagcacga tctgatggtt tagtaattaa attaatcatc
     2041 ttcagtgata atttagccct cttgcgcact aaaaaaatcg atctcgtcaa atttcagact
     2101 tatccatcag actatactgt tgtacctata aaggagcagt ggaatagcgt tcgcagaccg
     2161 taactttcag gcacttaccc tgaagtacgg ggctgtggga taaaaacaat ctggaggaat
     2221 gtcgtgcaaa cctttcaagc cgatcttgcc attgtaggcg ccggtggcgc gggattacgt
     2281 gctgcaattg ctgccgcgca ggcaaatcca aatgcaaaaa tcgcactaat ctcaaaagta
     2341 tacccgatgc gtagccatac cgttgctgca gaagggggct ccgccgctgt cgcgcaggat
     2401 catgacagct tcgaatatca ctttcacgat acagtagcgg gtggcgactg gttgtgtgag
     2461 caggatgtcg tggattattt cgtccaccac tgcccaaccg aaatgaccca actggaactg
     2521 tgggggtgcc catggagccg tcgcccggat ggtagcgtca acgtacgtcg cttcggcggc
     2581 atgaaaatcg agcgcacctg gttcgccgcc gataagaccg gcttccatat gctgcacacg
     2641 ctgttccaga cctctctgca attcccgcag atccagcgtt ttgacgaaca tttcgtgctg
     2701 gatattctgg ttgatgatgg tcatgttcgc ggcgtggtag caatgaacat gatggaaggc
     2761 acgctggtgc agatccgtgc taacgcggtc gttatggcta ctggcggtgc gggtcgcgtt
     2821 tatcgttaca acaccaacgg cggcatcgtt accggtgacg gtatgggtat ggcgctaagc
     2881 cacggcgttc cgctgcgtga catggaattc gttcagtatc acccaaccgg tctgccaggt
     2941 tccggtatcc tgatgaccga aggctgccgc ggtgaaggcg gtattctggt caacaaaaat
     3001 ggctaccgtt atctgcaaga ttacggcatg ggcccggaaa ctccgctggg cgagccgaaa
     3061 aacaaatata tggaactggg tccacgcgac aaagtttctc aggccttctg gcacgaatgg
     3121 cgtaaaggca acaccatctc cacgccgcgt ggcgatgtgg tttatctcga cctgcgtcac
     3181 ctcggcgaga aaaaactgca tgaacgtctg ccgttcatct gcgaactggc gaaagcgtac
     3241 gttggcgtcg atccggttaa agaaccgatt ccggtacgtc cgaccgcaca ctacaccatg
     3301 ggcggtatcg aaaccgatca gaactgtgaa acccgcatta aaggtctgtt cgccgtgggt
     3361 gaatgttcct ctgttggtct gcacggtgca aaccgtctgg gttctaactc cctggcggaa
     3421 ctggtggtct tcggccgtct ggccggtgaa caagcgacag agcgtgcagc aactgccggt
     3481 aatggcaacg aagcggcaat tgaagcgcag gcagctggcg ttgaacaacg tctgaaagat
     3541 ctggttaacc aggatggcgg cgaaaactgg gcgaagatcc gcgacgaaat gggcctggca
     3601 atggaagaag gttgcggtat ctaccgtacg ccggaactga tgcagaaaac catcgacaag
     3661 ctggcagagc tgcaggaacg cttcaagcgc gtgcgcatca ccgacacctc cagcgtgttc
     3721 aacaccgacc tgctctacac cattgaactg ggccacggtc tgaacgttgc tgaatgtatg
     3781 gcgcactccg caatggcacg taaagagtcc cgcggcgcac accagcgtct ggacgaaggt
     3841 tgcaccgagc gtgacgacgt caacttcctc aaacacaccc tcgccttccg cgatgctgat
     3901 ggcacgactc gcctggagta cagcgacgtg aagattacta cgctgccgcc agctaaacgc
     3961 gtttacggtg gcgaagcgga tgcagccgat aaggcggaag cagccaataa gaaggagaag
     4021 gcgaatggct gagatgaaaa acctgaaaat tgaggtggtg cgctataacc cggaagtcga
     4081 taccgcaccg catagcgcat tctatgaagt gccttatgac gcaactacct cattactgga
     4141 tgcgctgggc tacatcaaag acaacctggc accggacctg agttaccgct ggtcctgccg
     4201 tatggcgatt tgtggttcct gcggcatgat ggttaacaac gtgccaaaac tggcatgtaa
     4261 aaccttcctg cgtgattaca ccgacggtat gaaggttgaa gcgttagcta acttcccgat
     4321 tgaacgcgat ctggtggtcg atatgaccca cttcatcgaa agtctggaag cgatcaaacc
     4381 gtacatcatc ggcaactccc gcaccgcgga tcagggtact aacatccaga ccccggcgca
     4441 gatggcaaag tatcaccagt tctccggttg catcaactgt ggtctgtgct acgccgcgtg
     4501 cccgcagttt ggcctgaacc cagagttcat cggtccggct gccattacgc tggcgcatcg
     4561 ttataacgaa gatagccgcg accacggtaa gaaggagcgt atggcgcagt tgaacagcca
     4621 gaacggcgta tggagctgta ctttcgtggg ctattgctcc gaagtctgcc cgaaacacgt
     4681 cgatccggct gcggccattc agcagggcaa agtagaaagt tcgaaagact ttcttatcgc
     4741 gaccctgaaa ccacgctaag gagtgcaaca tgacgactaa acgtaaaccg tatgtacggc
     4801 caatgacgtc cacctggtgg aaaaaattgc cgttttatcg cttttacatg ctgcgcgaag
     4861 gcacggcggt tccggctgtg tggttcagca ttgaactgat tttcgggctg tttgccctga
     4921 aaaatggccc ggaagcctgg gcgggattcg tcgacttttt acaaaacccg gttatcgtga
     4981 tcattaacct gatcactctg gcggcagctc tgctgcacac caaaacctgg tttgaactgg
     5041 caccgaaagc ggccaatatc attgtaaaag acgaaaaaat gggaccagag ccaattatca
     5101 aaagtctctg ggcggtaact gtggttgcca ccatcgtaat cctgtttgtt gccctgtact
     5161 ggtaaggagc ctgagatgat taatccaaat ccaaagcgtt ctgacgaacc ggtattctgg
     5221 ggcctcttcg gggccggtgg tatgtggagc gcaattattg cgccggtgat gatcctgctg
     5281 gtgggtattc tgctgccact ggggctgttt ccgggtgatg cgctgagcta cgagcgcgtt
     5341 ctggcgttcg cgcagagctt cattggtcgc gtattcctgt tcctgatgat cgttctgccg
     5401 ctgtggtgtg gtttacaccg tatgcaccac gcgatgcacg atctgaaaat tcacgtgcct
     5461 gcgggcaaat gggttttcta cggtctggct gctatcctga cagttgtcac gctgattggt
     5521 atcgttacaa tctaacgtat cgccaatgta aatccggccc gcctatggcg ggccgttttg
     5581 tatggaaacc agaccttatg ttcaaaacga cgctctgcgc cttattaatt accgcctctt
     5641 gctccacatt tgctgcccct caacaaatca acgatattgt gcatcgcaca attaccccgc
     5701 ttatagagca acaaaagatc cccggtatgg cggtggcggt aatttatcag ggtaaacctt
     5761 attactttac ctggggctat gcggacatcg ccaaaaagca gcccgtcaca cagcaaacgt
     5821 tgtttgagtt aggttcggtc agcaaaacat ttacgggcgt gcttggtggc gacgctattg
     5881 ctcgagggga aatcaagtta agcgatccca caacaaaata ctggcctgaa cttaccgcta
     5941 aacagtggaa tgggatcaca ctattacatc tcgcaaccta cactgctggc ggcctgccat
     6001 tgcaggtgcc ggatgaggtg aaatcctcaa gcgacttgct gcgcttctat caaaactggc
     6061 agcctgcatg ggcgccagga acacaacgtc tgtatgccaa ctccagtatc ggtttgttcg
     6121 gcgcactggc tgtgaagccg tctggtttga gttttgagca ggcgatgcaa acacgtgtct
     6181 tccagccact caaactcaac catacgtgga ttaatgtacc gcccgcagaa gaaaagaatt
     6241 acgcctgggg atatcgcgaa ggcaaggcag ttcatgtttc gccaggggcg ttagatgctg
     6301 aaacttatgg tgtgaagtcg accattgaag atatggcctg ctgggtacga agcaatatga
     6361 atccccgtga tatcaacgac aaaacacttc agcaagggat acaactggca caatctcgct
     6421 actggcaaac cggcgatatg tatcagggcc tgggctggga aatgctggac tggccggtaa
     6481 atcctgacag catcattaac ggcagtggca ataaaattgc actggcagca caccccgtaa
     6541 aagcgattac gcccccaact cctgcagtac gcgcatcatg ggtacataaa acaggggcaa
     6601 ccggcggatt tggtagctat gtcgcgttta ttccagaaaa agagctgggt atagtgatgc
     6661 tggctaacaa aaactatccc aatccagcga gagtcgccgc cgcctggcag attctcaacg
     6721 ctctacagta aaatttcatc gggtccgaat tttcggacct tttctgcgct tttccttgct
     6781 gtcatctaca cttagaaaaa aaccagtaag gaaacattta tgcgcctgct ccctctcgtt
     6841 gccgcagcga cagctgcatt tctggtcgtt gcctgcagtt ctcctacgcc gccgcgtggc
     6901 gtaacagtag tgaataattt cgacgccaaa cgttatctcg gtacctggta tgagattgcc
     6961 cgttttgatc accgttttga acgtggactg gaaaaagtca ccgcaacata cagcctgcgt
     7021 gatgacggcg gcctgaatgt cattaataaa ggctataacc ctgacagagg aatgtggcag
     7081 cagagtgaag ggaaagcgta ctttaccggc gcaccaactc gcgctgcgct gaaagtgtca
     7141 ttctttggtc ctttctatgg cggttataac gttattgcac tcgatcggga ataccgccat
     7201 gcgctggttt gcggcccgga ccgcgactac ctgtggatac tctcccgcac gccaaccatt
     7261 tctgacgaag tgaaacagga gatgctggca gtcgcgaccc gggaagggtt tgatgtcagt
     7321 aagtttattt gggtacagca gcccggtagt tagtgagtgc tgagtttcag accaataatc
     7381 cccaatacga ttaacgccag actcgccagg cgcatcgggt tagcggactc accgagcagc
     7441 acaatgccgg tgatggccgc gccgactgcg ccaatacccg tccacacggc ataagccgtc
     7501 cctactggta acgatttcat cgcccaggca agtagcgcca tactgacaat catcgccgtc
     7561 acggtaataa cactcggcgt caaacgacta aagccgtggg tatatttcag gccaacggcc
     7621 cataccactt ccagcagacc agcaataact aagataatcc aggacatatc aggctccgga
     7681 gaattggggc cgtccccggt gaaaagaggc gtttgcaggt cgtcctgcaa agctgatggt
     7741 tgaacggata ttttgccagg tggcagggga atttcaacta ttaccttagt gactatcgca
     7801 gaaaaaacaa acggcacgac atacctgtcg taccgttatt gcttattgct attgctgcgc
     7861 tttcgttgct gcgccagaaa tcgcgttacc gccatcagaa atgtcttcac caacgccacg
     7921 cgtggtgttg caggcagtta atactgttga aagcaccaga acagaaaaga tcgctgcaat
     7981 tgtcttcttc accataacgt cttcctttta ggtaatgtta tttatgtttg cctatagcaa
     8041 attaataata gacaacatcg ccaaaaatga cggaaatctg atgat
//
LOCUS       DAZKHF020000905         2019 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2201S31241
            SAMN43267800-rid25106413.guided.434, whole genome shotgun sequence.
ACCESSION   DAZKHF020000905 DAZKHF020000000
VERSION     DAZKHF020000905.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267800
            Sequence Read Archive: SRR30307263
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2019)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2019)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 2019)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350581.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350581.2
            Genome Coverage       :: 151x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:21:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,652
            CDSs (total)                      :: 5,557
            Genes (coding)                    :: 5,320
            CDSs (with protein)               :: 5,320
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 237
            CDSs (without protein)            :: 237
            Pseudo Genes (ambiguous residues) :: 0 of 237
            Pseudo Genes (frameshifted)       :: 102 of 237
            Pseudo Genes (incomplete)         :: 130 of 237
            Pseudo Genes (internal stop)      :: 52 of 237
            Pseudo Genes (multiple problems)  :: 41 of 237
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2019
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267800-rid25106413.guided.434"
                     /serotype="O 26:H11"
                     /isolate="2201S31241"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-01"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            127..1086
                     /locus_tag="ACHOSC_RS28240"
                     /old_locus_tag="pgaptmp_005648"
     CDS             127..1086
                     /locus_tag="ACHOSC_RS28240"
                     /old_locus_tag="pgaptmp_005648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Shiga toxin Stx2 subunit A"
                     /protein_id="HGU4551334.1"
                     /translation="MKCILFKWVLCLLLGFSSVSYSREFTIDFSTQQSYVSSLNSIRT
                     EISTPLEHISQGTTSVSVINHTPPGSYFAVDIRGLDVYQARFDHLRLIIEQNNLYVAG
                     FVNTATNTFYRFSDFTHISVPDVTTVSMTTDSSYTTLQRVAALERSGMQISRHSLVSS
                     YLALMEFSGNTMTRDASRAVLRFVTVTAEALRFRQIQREFRQALSETAPVYTMTPGDV
                     DLTLNWGRISNVLPEYRGEDGVRVGRISFNNISAILGTVAVILNCHHQGARSVRAVNE
                     ESQPECQITGDRPVIKINNTLWESNTAAAFLNRKSQFLYTTGK"
     gene            1098..1367
                     /locus_tag="ACHOSC_RS28245"
                     /old_locus_tag="pgaptmp_005649"
     CDS             1098..1367
                     /locus_tag="ACHOSC_RS28245"
                     /old_locus_tag="pgaptmp_005649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Shiga toxin Stx2a subunit B"
                     /protein_id="HGU4551335.1"
                     /translation="MKKMFMAVLFALVSVNAMAADCAKGKIEFSKYNEDDTFTVKVDG
                     KEYWTSRWNLQPLLQSAQLTGMTVTIKSSTCESGSGFAEVQFNND"
     gene            complement(1665..1988)
                     /locus_tag="ACHOSC_RS28250"
                     /old_locus_tag="pgaptmp_005650"
     CDS             complement(1665..1988)
                     /locus_tag="ACHOSC_RS28250"
                     /old_locus_tag="pgaptmp_005650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001295666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0043856 - anti-sigma factor antagonist
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anti-adapter protein IraM"
                     /protein_id="HGU4551336.1"
                     /translation="MNWIVIDTVIQPACDISFSAIWCNIKLILWYQSDIFLPPGCIFT
                     PTRTGVILNNKELPVTIYNVTPFNKNVWNLIKSSQECPANTGNITDKCFNHRCILEIC
                     PYGRK"
BASE COUNT          534 a          402 c          490 g          593 t
ORIGIN      
        1 aatcggtcac tggttcgaat ccagtacaac gcgccatatt tatttaccag gctcgctttt
       61 gcgggccttt tttatatctg cgccgggtct ggtgctgatt acttcagcca aaaggaacac
      121 ctgtatatga agtgtatatt atttaaatgg gtactgtgcc tgttactggg tttttcttcg
      181 gtatcctatt cccgggaatt tacgatagac ttttcgactc aacaaagtta tgtctcttcg
      241 ttaaatagta tacggacaga gatatcgacc cctcttgaac atatatctca ggggaccaca
      301 tcggtgtctg ttattaacca caccccgccg ggcagttatt ttgctgtgga tatacgaggg
      361 cttgatgtct atcaggcgcg ttttgaccat cttcgtctga ttattgagca aaataattta
      421 tatgtggccg ggttcgttaa tacggcaaca aatactttct accgtttttc agattttaca
      481 catatatcag tgcccgatgt gacaacggtt tccatgacaa cggacagcag ttataccact
      541 ctgcaacgtg tcgcagcgct ggaacgttcc ggaatgcaaa tcagtcgtca ctcactggtt
      601 tcatcatatc tggcgttaat ggagttcagt ggtaatacaa tgaccagaga tgcatccaga
      661 gcagttctgc gttttgtcac tgtcacggca gaagccttac gcttcaggca gatacagaga
      721 gaatttcgtc aggcactgtc tgaaactgct cctgtgtata cgatgacgcc gggagacgtg
      781 gacctcactc tgaactgggg gcgaatcagc aatgtgcttc cggagtatcg gggagaggat
      841 ggtgtcagag tggggagaat atcctttaat aatatatcag cgatactggg tactgtggcc
      901 gttatactga attgccatca tcagggggcg cgttctgttc gcgccgtgaa tgaagagagt
      961 caaccagaat gtcagataac tggcgacagg cccgttataa aaataaacaa tacattatgg
     1021 gaaagtaata cagctgcagc gtttctgaac agaaagtcac agtttttata tacaacgggt
     1081 aaataaagga gttaagtatg aagaagatgt ttatggcggt tttatttgca ttagtttctg
     1141 ttaatgcaat ggcggcggat tgtgctaaag gtaaaattga gttttccaag tataatgagg
     1201 atgacacatt tacagtgaag gttgacggga aagaatactg gaccagtcgc tggaatctgc
     1261 aaccgttact gcaaagtgct cagttgacag gaatgactgt cacaatcaaa tccagtacct
     1321 gtgaatcagg ctccggattt gctgaagtgc agtttaataa tgactgaggc ataacctgat
     1381 tcgtggtatg tgggtaacaa gtgtaatctg tgtcacaatt cagtcagttg acagttgcct
     1441 gtcagactga gcatttgtta aaaaaatttc gcatggtgaa tccccctgtg cggaggggcg
     1501 actggtgtat aaggtgtcat ctctgacgac aacctggatc gagatacgcg ggttcaggga
     1561 caccggactg aactcaccgg gaggcacccg gcaccatgtt catggtgata cagaaatgcg
     1621 gcttcagccc ctctccggag gggcttttta tctgaatgat tctgttattt tcgcccgtat
     1681 gggcatattt ccagaatgca gcgatgatta aaacacttat ctgtgatatt tcctgtgttt
     1741 gcagggcatt cctggctgct tttaattaaa ttccagacgt ttttattaaa tggggtcacg
     1801 ttgtaaatgg tgacggggag ctctttatta ttgagtatta cgcctgtccg ggttggtgta
     1861 aaaatacagc ccggagggag gaagatatca gactgatacc ataatattaa ttttatgtta
     1921 caccatatgg ctgaaaatga tatgtcacat gctggctgga ttaccgtatc aatcactatc
     1981 cagttcattt gctttcctta ttttcttaag gttttaaat
//
LOCUS       DAZKHF020000906         1501 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2201S31241
            SAMN43267800-rid25106413.guided.500, whole genome shotgun sequence.
ACCESSION   DAZKHF020000906 DAZKHF020000000
VERSION     DAZKHF020000906.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267800
            Sequence Read Archive: SRR30307263
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1501)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1501)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 1501)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350581.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350581.2
            Genome Coverage       :: 151x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:21:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,652
            CDSs (total)                      :: 5,557
            Genes (coding)                    :: 5,320
            CDSs (with protein)               :: 5,320
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 237
            CDSs (without protein)            :: 237
            Pseudo Genes (ambiguous residues) :: 0 of 237
            Pseudo Genes (frameshifted)       :: 102 of 237
            Pseudo Genes (incomplete)         :: 130 of 237
            Pseudo Genes (internal stop)      :: 52 of 237
            Pseudo Genes (multiple problems)  :: 41 of 237
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1501
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267800-rid25106413.guided.500"
                     /serotype="O 26:H11"
                     /isolate="2201S31241"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-01"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            263..1210
                     /gene="stxA1a"
                     /locus_tag="ACHOSC_RS28255"
                     /old_locus_tag="pgaptmp_005651"
     CDS             263..1210
                     /gene="stxA1a"
                     /locus_tag="ACHOSC_RS28255"
                     /old_locus_tag="pgaptmp_005651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Shiga toxin Stx1a subunit A"
                     /protein_id="HGU4551337.1"
                     /translation="MKIIIFRVLTFFFVIFSVNVVAKEFTLDFSTAKTYVDSLNVIRS
                     AIGTPLQTISSGGTSLLMIDSGSGDNLFAVDVRGIDPEEGRFNNLRLIVERNNLYVTG
                     FVNRTNNVFYRFADFSHVTFPGTTAVTLSGDSSYTTLQRVAGISRTGMQINRHSLTTS
                     YLDLMSHSGTSLTQSVARAMLRFVTVTAEALRFRQIQRGFRTTLDDLSGRSYVMTAED
                     VDLTLNWGRLSSVLPDYHGQDSVRVGRISFGSINAILGSVALILNCHHHASRVARMAS
                     DEFPSMCPADGRVRGITHNKILWDSSTLGAILMRRTISS"
     gene            1220..1489
                     /gene="stxB1a"
                     /locus_tag="ACHOSC_RS28260"
                     /old_locus_tag="pgaptmp_005652"
     CDS             1220..1489
                     /gene="stxB1a"
                     /locus_tag="ACHOSC_RS28260"
                     /old_locus_tag="pgaptmp_005652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Shiga toxin Stx1a subunit B"
                     /protein_id="HGU4551338.1"
                     /translation="MKKTLLIAASLSFFSASALATPDCVTGKVEYTKYNDDDTFTVKV
                     GDKELFTNRWNLQSLLLSAQITGMTVTIKTNACHNGGGFSEVIFR"
BASE COUNT          399 a          258 c          361 g          483 t
ORIGIN      
        1 gagtttgcca gcctccccca gtggctggct tttttatgtc cgtaacatcc tgtgtatcaa
       61 taaatgttgt tatctacgta cgtcaagtag tcgcatgaga tctgaccaga tatgttaagg
      121 ttgcagctct ctttgaatat gattatcatt ttcattacgt tattgttacg tttatccggt
      181 gcgccgtaaa acgccgtcct tcagggcgtg gaggatgtca agaatatagt tatcgtatgg
      241 tgctcaagga gtattgtgta atatgaaaat aattattttt agagtgctaa cttttttctt
      301 tgttatcttt tcagttaatg tggtggcgaa ggaatttacc ttagacttct cgactgcaaa
      361 gacgtatgta gattcgctga atgtcattcg ctctgcaata ggtactccat tacagactat
      421 ttcatcagga ggtacgtctt tactgatgat tgatagtggc tcaggggata atttgtttgc
      481 agttgatgtc agagggatag atccagagga agggcggttt aataatctac ggcttattgt
      541 tgaacgaaat aatttatatg tgacaggatt tgttaacagg acaaataatg ttttttatcg
      601 ctttgctgat ttttcacatg ttacctttcc aggtacaaca gcggttacat tgtctggtga
      661 cagtagctat accacgttac agcgtgttgc agggatcagt cgtacgggga tgcagataaa
      721 tcgccattcg ttgactactt cttatctgga tttaatgtcg catagtggaa cctcactgac
      781 gcagtctgtg gcaagagcga tgttacggtt tgttactgtg acagctgaag ctttacgttt
      841 tcggcaaata cagaggggat ttcgtacaac actggatgat ctcagtgggc gttcttatgt
      901 aatgactgct gaagatgttg atcttacatt gaactgggga aggttgagta gcgtcctgcc
      961 tgactatcat ggacaagact ctgttcgtgt aggaagaatt tcttttggaa gcattaatgc
     1021 aattctggga agcgtggcat taatactgaa ttgtcatcat catgcatcgc gagttgccag
     1081 aatggcatct gatgagtttc cttctatgtg tccggcagat ggaagagtcc gtgggattac
     1141 gcacaataaa atattgtggg attcatccac tctgggggca attctgatgc gcagaactat
     1201 tagcagttga gggggtaaaa tgaaaaaaac attattaata gctgcatcgc tttcattttt
     1261 ttcagcaagt gcgctggcga cgcctgattg tgtaactgga aaggtggagt atacaaaata
     1321 taatgatgac gataccttta cagttaaagt gggtgataaa gaattattta ccaacagatg
     1381 gaatcttcag tctcttcttc tcagtgcgca aattacgggg atgactgtaa ccattaaaac
     1441 taatgcctgt cataatggag ggggattcag cgaagttatt tttcgttgac tcagaatagc
     1501 t
//
